LOCUS       DASTMK010000001        10250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_288034, whole genome shotgun sequence.
ACCESSION   DASTMK010000001 DASTMK010000000
VERSION     DASTMK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288034"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1912
                     /locus_tag="VFQ79_00005"
     CDS             104..1912
                     /locus_tag="VFQ79_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0138054.1"
                     /translation="MQVAHEHSAADSQTGTVQSGKLIVLTAPLTETVDHAGYFIQMAL
                     ASLPKRMEAVINQKYPKWRELERNEDGSARYVPAGLRLVEASLLREFSAEDIVCCYPE
                     DLPRFIGPSTRAVGVTTHNPLGVTFAAGVYASIFGSSREPINSHYAKELFRTLQASPY
                     RSSFKVIVGGSGGWQIAETGSFEELGVDCVVEGRSESKDVLELFRKAVRSEDLPRFAR
                     VSHPTNRESLLTPHKRTTFGVVEMTTGCGRRCHFCLPDLNPQIDFSKQSIMEAVRANV
                     REGNSQISLATEDMFIWGQVHTDTPFYFPNREALIDLFRDVVSTPGVKHHLLSHCTMA
                     PSVVDPKLIEQMSEVLLDKSPVKLPALSTHPEGRILSPLIGMETASVRMAKQIMPGKA
                     VPFPIDEWPSVLIQGLTILNRNNWFPVMTLMIGNPGETDADCMATLDLLYEVERRGLF
                     AFFVPSIFTPLHDTRFAAKKGVVESREMSALQWQLMMKCWKMSMKPALHSWWGPMAWR
                     IGALGIWAWKLRKLNGPNFTWPLFLFAGALPENLMARMGKIYRGRPLEIKSRKELLAT
                     IKPQHWKYLRKDNGDLPDGWEQQPASEPLRVLRASS"
     gene            1925..3304
                     /locus_tag="VFQ79_00010"
     CDS             1925..3304
                     /locus_tag="VFQ79_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138055.1"
                     /translation="MKRAAFLLLFAALVSAAGELRVGRASLRITPETGTPMGSSYGLT
                     ISTGVHDDLYIKTLALEKDGARAALVACDFISLRPDVVVEARKLIQQSAGLSPGQVIL
                     SATHSHCGPQMHRPFLELLDEESLRLGVLFREQLPQRISDCVRQAFADLRPARVSVGI
                     GKESSISFNRRFHMKDGSVRMNPGRRNPDAIRPAAGIDPDVNVVYFTSTGGEPLATHV
                     NFAMHPAIAGGSEFSADYPGVLASLLRKVKGGGMLSLFTMGTAGDINHLDVSKEKQLS
                     NHDETSRVGMVLTGEVLKTYTKLQPVDGTLGVARTTLHLPAREPREGEAAKAQGIFSR
                     YRAGRDGPPFQDVVWAWRTIETAALEDKHLSADVQVISLGDQLAWVALPGEIFVDLGL
                     AIKKASPFPYTIISTMSASGTISYVPTRKAFAEGSYEVTSARIAAGGGEMLVDAAVRL
                     LKKLKAEAR"
     gene            complement(3317..4687)
                     /locus_tag="VFQ79_00015"
     CDS             complement(3317..4687)
                     /locus_tag="VFQ79_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138056.1"
                     /translation="MSKLAAIFLLTSAAFAAEFRAGRAAVNINPPTGWPGGPAGIHDD
                     LHAKALVFEKDGMKTAIVVCDLAVIQKDVVEAARLLIERENGIPPRNVMISATHVHSA
                     FELPIWWEWETAPPVMMEYRASLPAKIAAAVRQAHQDLQPVRVSAGIGHEDSLSFNRR
                     FLMKDGSARMNPGKRNPDIVRPLGPIDPDVMVACIESVDGKPLATFVNFAMHLDTVGG
                     REVSADFPYTLSKLLAEAKGPEMLTIFGIGAAGNINHIDVSSAKPQKGHGEAARIGTV
                     LAAEVLKTFERMTPVEPATLRAAYEKVEFELPAIGPGDIERAEALRSRLGSRDKPSFL
                     QQMWMQRILETAKRGGKPIETEVQAIAVGDQLAWVSLPSEVFVEIGLAIKKASPFPFT
                     IVDTLASDWLRYTPNRKGYAEGAYEAINTRGAPGSGERLADAATRLLLELRTPYPGAF
                     GALPAK"
     gene            4796..5614
                     /locus_tag="VFQ79_00020"
     CDS             4796..5614
                     /locus_tag="VFQ79_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0138057.1"
                     /translation="MPTTRKPNAAAVGSRYMVPVVRSTFRILEELSGAGAAGLNELTH
                     RTGVAKSTVFRILSTLHSLGYVLRDERRNYHVSPRVADLVSDTARGETLSNTAMPLML
                     KLRNEFGETVNLGELNFDKVVYLAVVPSDYALRFSERPGISVPAHASALGKAILAFSP
                     PEVVDGLISGRELEILTRNTITKVDEFRRELKRVRNRGFALDREETSLLATCIGAPIF
                     DAHGHPLAALSISGPSSRFNPRRNSPVVEALLAATAEISRQFRWRSEAELKPTG"
     gene            complement(5658..8825)
                     /locus_tag="VFQ79_00025"
     CDS             complement(5658..8825)
                     /locus_tag="VFQ79_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0138058.1"
                     /translation="MPAILLAAPLLAQSDLATIRGTITDPTGAVVPNVSVELVHTATN
                     TTRQTLTNQSGDFEVPYLSPGTYRLTATASGFKNFVATNIILTARETRRVDAALEVGA
                     VGAEVTVSAGVAMIATEGAQIADGFGREAWVDSPLSQSFFPQAFMTTLPNIQTQQGGW
                     SLRFAGQPPAQVAENMDGVTNDGTVNLVQNMQDFEDLQVVAVNNSAEFSRVAQFSMAS
                     KGGTNEFHGRVYYDLINSALGARSFFTPTKIPYKEHRGGANLGGPIVPNKLFFYGGYS
                     LVRIPSASFYNRNVATEAMRAGDFSSLLNQANPVRIRDPLTGQPFPGNIIPQNRINST
                     SLGIQNNYIPHPNQGSPDSIFQNYGFRHPYPVDLYKWDSVTARLDYYISSKNQLFGRF
                     INRLTPYVLNGPFEQLGTWTRSRDHHSIVITDTHTFSPSLVNSFHWGWIKDYFIDGDE
                     TDGFLPTRGDEAIQQIGLQGVNRGGHSEMGFPTITLPGFTTLSQQPGGVNLNRRDFEF
                     ADSLTWSVGRHVMKFGGELRTFRDFRQGITANNWGSFNFDGSITGYSYADFLLGLPFS
                     SARLDPLVDRTQRAYELGFFVADTFKVSRKLTLDFGLRWDYFRHTMYEDELQYNWDRD
                     TGNVIVPADARDKVSPLYSPNINIVTGDVFPSPKKTNFRPRIGLAYRFQENFVLRGGY
                     GQYTEALGNFHRGDGGGPFQIAETYFNSIQNGVPLLSFPNPFPATLVGAQVPNQSVTG
                     YPMETENGVIHQYNVSLEKEVLGFGLRFSYIGSRSKGLNYNLQLNKPQPSLIPFTQAR
                     RPRPEFVGTTFALSDGESKYDAGQIEVQRRSGPLVFSAHYTMAHGMNNFSNLENPYDH
                     YAWSRDPFNSRHRAVINAQYTLPVGRGKRFLTSAPGAVDAVLGGWQIGWISYFQGGQY
                     FSPSFSGSDPSNTNTSGGIPDRIGDGNLSTDQRQPDRWFDASAFAPPAPGRFGNAGVA
                     ILEGPGLNLHHMSAIKEFRITERVKFVTQGMITNIFNHSHFDFPSANISVPNTVARVT
                     QLREGAGGREMSGPRNVQLRFRIEF"
     gene            complement(9015..>10250)
                     /locus_tag="VFQ79_00030"
     CDS             complement(9015..>10250)
                     /locus_tag="VFQ79_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138059.1"
                     /translation="VNRRYFIGQSVAASMTASAWQNTPGANGRIRVANIGCGRRGLLR
                     ELIQVKDATNIEIAAVCDTWKQKLEKAAADVKEHTGKDPAQVVDYRDVLARKDIDAVI
                     IGTPDHQHCTMLIAAVEAGKDVYIEKPMAMNMKELVRAYDAVKRTGRIVQVGTQIRSY
                     RTTRGAREFVTSGGIGKILKAEQVRNSYMPYWMNYGGAAYMDQKPTEADVDWKAFLMQ
                     RPARSFNASQYQNWYGYREFSSGPHTNLMVHFIDTMHYVTGASIPQRVTALGGTYRWK
                     NEFTAPDSVEVALEYPEGFLVRYCTMLGNGANNYAKFFGTRGTLDAKSLSPKGPWTAS
                     GQGAGDPDKLQQDVNVPEAETVHHMQNFLESVRSRTQPIAPIEAGYAHSVAVIMADQA
                     LTTGRRMIYDPDKREVRAG"
BASE COUNT         2038 a         2989 c         3080 g         2143 t
ORIGIN      
        1 cgagcagggt aagtctgata cgactgaagg cagccgggtt tgtatagtac catcgcagga
       61 gtatccaata actggtttgc ggtagctact tatagaggga taaatgcagg ttgctcacga
      121 acatagtgct gccgattctc aaacagggac ggtgcagtcc ggcaagttga ttgttctcac
      181 ggccccgctt acagagacgg tagatcatgc cggctacttt attcagatgg cgctggcatc
      241 gctgccgaaa cgcatggaag cggttatcaa ccagaagtat cccaagtggc gcgagctcga
      301 acggaacgag gatggctcgg cgcgctacgt gcccgctgga ctacggctgg tggaagcttc
      361 cctcctgcgc gagttttccg cggaggatat tgtctgctgt tatccggagg acttgccgcg
      421 attcatcggc ccgtctacgc gcgccgtcgg cgttacaact cacaacccgt tgggcgtgac
      481 gttcgcggcc ggcgtctacg cgtcgatttt tggctcctcc cgcgagccca tcaactcgca
      541 ctacgcaaag gaactgttcc ggacactcca ggccagcccg tatcgatcga gcttcaaagt
      601 gatcgtcggc ggttcgggcg gctggcagat cgcggagacc ggaagtttcg aggagttggg
      661 cgtcgactgt gtagtggaag gccgcagcga atcgaaggac gtactggagt tgtttcgtaa
      721 agcggtgcgc agcgaagatc tgccacgctt cgcgcgggtg agccatccca caaaccggga
      781 aagcctgtta accccccaca aacggaccac gtttggggtg gtcgagatga cgaccggatg
      841 cggccggcgc tgtcacttct gtttgcccga tctgaatccg cagatcgact tctcaaaaca
      901 gagcatcatg gaggctgttc gcgcgaacgt gcgggagggc aactcgcaga tctcgctggc
      961 gaccgaggac atgtttatct ggggacaggt ccacacggac acgccgttct actttcccaa
     1021 ccgggaagcg ctgatcgatc tttttcgcga cgtggtaagc actcccggcg tcaaacacca
     1081 cctgttgagc cactgcacca tggctccatc ggttgtggat ccaaagctca tcgagcagat
     1141 gtccgaagtt ctgctcgaca aaagtccggt caagctgccg gcgttgagca cgcatccgga
     1201 aggccgcatt ctatcgccct tgatcggcat ggaaaccgca tcggtgcgga tggcgaaaca
     1261 gatcatgccg gggaaagcgg tcccatttcc gatcgacgag tggcccagcg tgctgatcca
     1321 aggcctgacg atcctgaacc gcaacaactg gtttccggta atgacgttga tgatcggcaa
     1381 tcccggcgaa acggatgccg attgcatggc cacgctcgat ctgctctacg aagtggagcg
     1441 gcgcggactg ttcgcgtttt tcgttccatc gattttcaca ccgctgcacg acacgcgttt
     1501 cgcggccaag aagggggttg tcgagagccg cgaaatgtcg gcgctgcaat ggcaactgat
     1561 gatgaagtgc tggaagatga gcatgaagcc cgcgctgcac agttggtggg gcccgatggc
     1621 gtggcggatt ggcgcattgg gaatctgggc ctggaaattg cgcaaactga acggtccgaa
     1681 cttcacctgg ccgttatttc tgttcgccgg cgcccttcct gaaaacctga tggccaggat
     1741 gggtaagatc tatcgggggc gtccgcttga aatcaagagc cgcaaagaac tgcttgccac
     1801 catcaagccg cagcattgga aatacctgcg gaaagataat ggcgaccttc cggatggttg
     1861 ggagcagcag ccggcctcgg agccgctgcg cgttctgcgt gcgtcgagtt gagtcttcag
     1921 gcgtatgaag agagccgcat ttctactgtt gttcgcggcc ttggtatcgg ctgccgggga
     1981 acttcgcgtg gggcgggcgt cgctgcgtat caccccggaa acgggtacac ccatgggaag
     2041 ctcttacggc ctcacgatct caaccggtgt gcacgatgac ctttacataa agacgctggc
     2101 gcttgaaaag gacggtgctc gggcggcact ggtagcatgt gatttcatca gcctgcggcc
     2161 tgacgtagtc gtcgaggcgc gcaaattgat tcaacagagt gcgggtttga gtcccgggca
     2221 ggtgattctc agcgccacgc acagccattg cggtccgcag atgcatcgcc cgtttttgga
     2281 actgctggat gaggaatcgc tgcggctggg cgtcctgttc cgcgagcagt tgccgcaacg
     2341 aatctcggat tgcgtacgcc aggcattcgc tgatctgcgc ccggcgcgcg tctccgttgg
     2401 cattggaaag gaatcgtcga ttagcttcaa ccggcgattc cacatgaaag acggatctgt
     2461 ccgcatgaat ccaggacgcc gcaatcccga tgcgatccgg ccggcggcgg gcatcgatcc
     2521 cgatgtgaat gtcgtctact tcacgtccac gggaggcgaa ccgctcgcca cgcatgtaaa
     2581 cttcgccatg cacccggcca ttgccggcgg cagcgagttt tccgccgatt accccggtgt
     2641 cctcgcgagt ctgctgcgca aggtaaaggg cgggggaatg ctgagcctgt tcacgatggg
     2701 caccgccggc gacatcaacc accttgatgt aagtaaagag aaacaactgt ccaatcatga
     2761 cgagacatcg cgcgtcggaa tggtgctgac gggtgaagtg ctgaaaacat acacaaaact
     2821 acagcccgtg gatggaaccc ttggtgtcgc gcggaccacg ttgcaccttc cggcccggga
     2881 gccgcgggaa ggagaggccg ccaaggcgca gggcattttt tcccgatacc gcgccggccg
     2941 tgatggtcca ccttttcagg atgtcgtctg ggcctggcgc accatcgaaa ccgcagcgct
     3001 ggaagacaag catctgagcg ccgatgttca ggtgatctct ttgggagatc agcttgcctg
     3061 ggtggcgctg ccgggcgaga tctttgtcga cctgggactt gccatcaaga aagcgtcacc
     3121 gttcccttac acgattatca gcacgatgtc ggcgagcgga acgatcagtt acgtgcctac
     3181 tcgcaaggca ttcgccgagg gctcctacga agtgacgagc gcccggattg ccgcgggtgg
     3241 cggcgaaatg ctggttgatg cagcggtgag actgctgaag aagttaaagg ccgaagcacg
     3301 gtgacctgtc ctctgcctac tttgccggca acgctccaaa ggctcccgga tagggcgttc
     3361 gcagctccag aagaaggcgc gtggccgcgt ccgcgagacg ctcaccggaa ccgggggcgc
     3421 cgcgggtgtt gatggcttcg taggcccctt cggcgtagcc tttgcggttg ggcgtgtagc
     3481 gaagccaatc gctcgccagc gtatcgacaa tcgtaaaagg aaacggtgac gctttcttga
     3541 tggcaaggcc gatctcgacg aacacctcgc tcggaagcga tacccaggcg agttggtcgc
     3601 cgaccgcgat cgcctgaacc tccgtctcta tgggcttgcc gccgcgcttt gcggtttcca
     3661 gtatccgctg catccacatc tgctgaagga aggagggctt gtccctgctc ccgagccgcg
     3721 agcgcaacgc ttccgcccgt tcgatgtcgc ccgggccaat agcaggcagc tcgaactcca
     3781 ccttttcgta tgcggcacgc agggtggccg gctctacagg cgtcatgcgt tcgaacgttt
     3841 tcagtacctc ggcagcgagc acggtgccga tccgcgccgc ctcaccatgt cccttctgcg
     3901 gtttggcgct gctcacgtca atatggttga tattgcccgc cgcgccgatc ccgaagatgg
     3961 tcagcatctc cggccctttc gcttccgcga gaagcttcga caaggtgtac gggaaatccg
     4021 ccgatacttc cctgccgccg acggtatcga ggtgcatggc gaaattaaca aacgtagcca
     4081 gcggctttcc gtccacgctt tcgatgcagg ctaccatcac gtccggatca atgggaccaa
     4141 ggggccgcac aatatccgga ttgcgcttgc ccggattcat gcgcgcgctg ccatccttca
     4201 tcagaaaccg gcgattaaag ctcagactgt cttcgtgccc gatgccggcc gacacgcgta
     4261 ccggttgcag atcctggtgc gcctggcgca ccgcggcggc aattttcgcc ggaagcgatg
     4321 cgcggtactc catcatgacg ggcggtgcgg tctcccattc ccaccagatg ggcaactcaa
     4381 aagcggagtg cacgtgggtg gcgctgatca tcacgttcct tggcgggata ccgttctccc
     4441 tttcaatgag gagacgggca gcctcgacga catctttctg tatgacggct agatcgcaca
     4501 ccacgatggc agttttcatc ccgtctttct cgaacacgag ggccttcgcg tgcagatcgt
     4561 cgtgaatgcc ggccggaccg ccgggccatc ccgtgggagg attgatgttc acagcggctc
     4621 tgcccgcgcg aaactccgcg gcaaaggctg cggatgtcaa caaaaagatc gcggccagct
     4681 tactcattgg gaaatgtcgc ccctccggtt cgccccagaa tagatttcac ccgccgggct
     4741 tgtcaatgat ggaatcttcg tcatttttct attgaaatgt gacatactga gtccgatgcc
     4801 gacaacccgc aaaccgaacg ctgccgccgt tggatcccgc tacatggtgc ctgtggtgcg
     4861 gagcaccttt cgcattctcg aggaactgtc cggcgcgggt gccgcgggtt tgaatgagct
     4921 tacgcatcga accggcgtag caaaatccac tgtatttcga atcctcagca cacttcatag
     4981 cctgggctat gtgctgcgcg acgagcgccg caattaccat gtcagcccca gggtggcaga
     5041 cctggtcagc gacacggccc gcggcgagac gctatcgaat acggcgatgc cgctcatgct
     5101 gaaattgcgc aatgaattcg gcgagaccgt gaacctggga gaattgaatt tcgacaaagt
     5161 ggtctacctg gccgttgtgc ccagcgatta cgcgctacgg ttctccgagc ggccggggat
     5221 ttccgttccg gcgcacgcct ccgcgctggg gaaagccatt ctcgcgttct cgcccccgga
     5281 ggtcgtagac gggctcatca gcggtaggga actggagata ctcacccgga ataccatcac
     5341 caaggtcgac gagttccggc gggaactcaa gcgggtacgc aatcgagggt tcgcattgga
     5401 ccgggaggaa acgtcactgc tcgcaacctg tatcggcgct cccatcttcg atgcgcacgg
     5461 gcatccgctc gccgcgctca gcatttccgg acccagttcg cgtttcaatc cgcggcgcaa
     5521 ttcacctgtt gtggaggcgc tgctcgcggc cacggcggaa atctcacgcc agttccgctg
     5581 gcggtcggaa gcggaactga agccaaccgg ataagagtgg gcgcgcctgg tagcagggcg
     5641 cccactgtat tagcggctca gaactcgatg cggaaccgca gttgcacgtt tcgcggaccc
     5701 gacatctccc ggccgcccgc gccttcgcgg agttgggtga cgcgcgcgac ggtgttcgga
     5761 accgagatgt tcgcactcgg aaaatcgaag tgtgagtgat tgaagatatt cgtgatcatg
     5821 ccctgcgtca cgaatttcac ccgctccgta attctgaact ccttgatagc gctcatatga
     5881 tgcaggttca gacccgggcc ctccaagata gccacaccgg cgttgccaaa acggccgggg
     5941 gccgggggcg cgaatgcact tgcatcaaac cagcgatccg gttggcgctg gtccgtagat
     6001 agattcccat cgccgatccg gtcgggaatt ccgccggagg tattggtgtt cgaaggatcg
     6061 gaaccgctga agctgggtga gaagtactgc ccaccctgga aatacgagat ccatccgatc
     6121 tgccagccgc ccagcactgc atccaccgca ccgggtgcgg aggtaaggaa tctcttaccg
     6181 cggccgaccg gcagcgtgta ctgcgcgttg atcacggccc gatgccgcga attgaacggg
     6241 tcccggctcc aggcataatg atcgtacggg ttttccaggt tcgagaagtt gttcatcccg
     6301 tgtgccatcg tgtaatgcgc actaaaaacc aggggacccg aacgccgctg cacttcgatc
     6361 tggccggcgt cgtacttcga ttctccatcg ctcagggcga aggtcgtccc gacaaattcg
     6421 gggcggggcc gcctggcctg cgtgaaagga atcaggctcg gttgcggctt attgagttgc
     6481 aggttgtagt tcaagccttt gctgcgcgag ccgatgtagg aaaaacgcag cccgaagcca
     6541 agcacttcct tctccaagct cacgttgtac tggtgaatga cgccgttctc cgtttccatc
     6601 gggtagccgg tcacgctttg gttggggacc tgggcaccaa cgagggtcgc cggaaagggg
     6661 ttggggaatg acagcagcgg aacgccattc tggatgctgt tgaagtaggt ttcggcgatc
     6721 tggaacgggc cgcctccatc tccgcggtgg aaattgccca acgcttccgt gtactgcccg
     6781 tatccgccac gcaaaacgaa attctcctga aaacgatatg ccaggcctat acgcggccgg
     6841 aagtttgttt tcttgggcga cgggaacacg tctccggtca caatgttgat attgggcgaa
     6901 tacagcgggc ttactttatc gcgcgcatcc gcaggcacaa tgacgtttcc agtgtcccgg
     6961 tcccagttgt actgaagctc atcctcgtac atggtgtgcc ggaagtaatc ccagcgtagt
     7021 ccaaagtcca gcgtcagttt acggctcacc ttgaacgtgt cggcaacgaa gaaacccaat
     7081 tcgtacgccc gctgggtccg gtccactagg gggtcgagcc gcgcgctcga aaagggtagt
     7141 cctagcagaa agtccgcata gctgtagccg gtgatggagc cgtcaaagtt gaagctgccc
     7201 cagttattcg ctgtgatacc ctgtctgaag tcgcgaaacg tccgcaattc gcctccgaac
     7261 ttcattacat gccgtcccac gctccaggtg agcgagtcgg cgaactcgaa gtcgcggcga
     7321 ttcaggttca cgccgcccgg ctgttgcgac aacgtcgtga atccgggaag tgtgatcgtc
     7381 ggaaagccca tttcgctgtg gccgccgcgg ttcacgcctt ggaggccgat ctgctggatc
     7441 gcctcgtcac cgcgcgtggg caggaaaccg tcggtctcgt cgccatcgat gaagtagtct
     7501 ttgatccatc cccaatggaa cgaattcacc agcgaaggag aaaaggtgtg cgtgtcggtg
     7561 atgacaatgg agtgatggtc gcggcttctg gtccaggtac cgagttgctc gaaaggacca
     7621 ttcagcacat acggcgtcag ccggttgata aagcgtccga atagttggtt tttgctggag
     7681 atatagtagt caaggcgcgc cgtcacgctg tcccatttat aaaggtcgac gggatagggg
     7741 tgccggaaac catagttctg gaaaatggag tccgggctgc cctggttcgg gtgcggaatg
     7801 tagttgttct gtattcccag cgaagtcgag ttgatgcggt tctgcgggat gatgttaccc
     7861 gggaaaggct gacccgtgag cgggtctcgg attcgcaccg gattcgcctg gttcagcagt
     7921 gacgagaaat ctcccgcacg catcgcttcg gtggccacgt tcctgttgta aaaactcgcg
     7981 ctgggtatcc ggaccaggct gtagccgcca tagaagaaga gcttgttggg gacaatcggt
     8041 ccgcctaaat tagctccccc acggtgttcc ttgtacggta ttttggtcgg cgtgaaaaag
     8101 cttcgcgcgc caagcgccga gttgatcagg tcgtaataca ctcgtccgtg aaactcgttc
     8161 gtccctccct tgctcgccat ggaaaattgc gcgacgcggg agaattccgc cgaattattg
     8221 actgccacca cttgcaggtc ttcgaaatcc tgcatattct gcaccaggtt caccgtaccg
     8281 tcgttggtga cgccatccat gttctccgca acctgcgcgg gaggctggcc ggcgaaccgc
     8341 aggctccatc cgccctgctg cgtctggata ttcggcagcg tggtcatgaa agcctgcggg
     8401 aagaaactct ggcttaacgg gctgtccacc catgcttcac ggccgaaacc atcggcgatc
     8461 tgggcgcctt cagtggctat catggccacg ccggccgaca ccgtaacctc cgcacccacc
     8521 gcacccacct ccagggcggc gtccacacgg cgggtttccc gcgcggtcag aataatgttt
     8581 gtggcgacga aattcttgaa accggaagct gtcgccgtaa gccggtaagt acccggcgag
     8641 agatagggta cttcgaaatc accactttgg tttgtcagcg tctgcctggt ggtattggtc
     8701 gcggtatgta ccagttccac cgaaacgttc ggtacaacgg ctccagtcgg atccgtgatg
     8761 gtgccacgga tggtagcgag gtccgactgc gccaggagcg gcgcagccag taaaatcgcc
     8821 ggcaacgtga gaaggataca acttctccac attcgcgtgg gtgtgtgcat tggtttcccc
     8881 ttcagaaggc ttaggaaatg tataacccac gcgccgccgc atgtcaacac agaaacgtga
     8941 aaaccgtatc agaaatgacg gacagtacgt aaaatctgcc gcgcaagcgc cactgcacgg
     9001 ctatgagacc ccatttagcc ggcacgcact tcgcgcttgt ccggatcgta gatcatccgg
     9061 cggcccgttg tgagcgcctg atcggccatg atgaccgcaa ccgaatgcgc atagccggcc
     9121 tcgatgggcg caatgggctg ggttcgagat cgtacggatt ccaggaaatt ttgcatgtgg
     9181 tgcaccgtct cggcctccgg cacgtttaca tcctgctgta gtttgtcggg atcgccagca
     9241 ccctggccgc tggcggtcca tggccccttg ggagaaaggc tcttggcgtc gagagtgcca
     9301 cgcgtgccaa agaacttcgc gtaattattg gcgccgtttc ccagcatagt gcagtagcgg
     9361 actagaaagc cctcgggata ctcgagcgcc acctcgaccg agtccggtgc cgtaaattca
     9421 ttcttccagc ggtaagttcc acctagcgca gttacgcgtt gcgggatgct ggcgccggtg
     9481 acgtaatgca tcgtatcgat gaagtgcacc atcaggttgg tgtgcggccc actcgagaat
     9541 tcgcggtagc cgtaccagtt ctgatactgg ctggcattga atgagcgggc aggccgctgc
     9601 atcaggaaag ccttccagtc cacatcggct tcggtgggct tctggtccat ataagcggcg
     9661 ccgccgtaat tcatccaata cggcatgtag ctgttgcgca cctgttctgc cttgaggatc
     9721 ttgccgattc cgccggacgt aacgaactcg cgggcccccc tggtagtgcg gtagctgcgg
     9781 atctgtgtgc cgacctgcac gatgcgtccg gtgcgcttga cggcgtcgta agcgcgcacc
     9841 agttccttca tgttcatcgc catcggtttc tcgatgtaaa cgtctttgcc cgcttcgacg
     9901 gcggcgatca acatggtgca atgctggtgg tccggcgtgc caatgataac cgcgtcgatg
     9961 tctttccgtg ccagcacgtc gcgataatcc accacctgcg cgggatcttt cccggtgtgc
    10021 tccttcacgt ccgccgcggc tttctcgagc ttctgtttcc acgtgtcgca gaccgccgcg
    10081 atttcaatgt tcgtggcatc cttgacctgg atcagttcgc gcaacaggcc gcgccgcccg
    10141 catccgatgt tggcgacccg gatgcgcccg ttcgcgccgg gagtgttctg ccaggcggaa
    10201 gccgtcatgg acgctgccac agattgtcca atgaagtatc ttcggttcac
//
LOCUS       DASTMK010000002         5947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_512049, whole genome shotgun sequence.
ACCESSION   DASTMK010000002 DASTMK010000000
VERSION     DASTMK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512049"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFQ79_00035"
     CDS             <1..227
                     /locus_tag="VFQ79_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEU0138060.1"
                     /translation="DGDYVLIERASEIHDGEIVVAVVSKSEATLKRFYREPNGMARLQ
                     PANAAMEPIVVPLEDLEIQGRLVAVLRKFR"
     gene            283..2298
                     /locus_tag="VFQ79_00040"
     CDS             283..2298
                     /locus_tag="VFQ79_00040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEU0138061.1"
                     /translation="MTGSSRNGWITSLYRKVGIAEKVFVVLFVLWLVFLLASPASTIR
                     LFLQFAMFAAGMVVLIRYLRRWVQSAIWRLRNRLYVVYLFIAVVPVCLILILGGLAAY
                     LIASEVSVYLVTSELNRRADSLGNLVEWLVRAPVAQRSAFLQNAGNYGRNRFPGLELI
                     IRGSGGGSFPASNKLEPPPGDWPDQNGLVLKDEILYAWAHIRRNDVDVTALVPLTQDY
                     LSELVPNLGEVNYTLAPAETGNPPVPNSGVLFQVDGTNIRAPAPQAGRKSQLGVAVNR
                     FDKEVLWFSLMPAAVWDAPGTTRKVIIAVRTRFSAVRQAISTEATSTERAGTDQNLLF
                     ILFTVVTGLFLGVELIALVIGVSLTRTITGAVHNLYEGTQKVMKGEFSHRIEVKGNDQ
                     LADLGISFNRMTENLEQLVVVAKEKERLQAELELAREVQAQLYPKVMPTLKTLRIVAT
                     CNPARLVSGDYFDYQCLEDTQVALALGDVAGKGISAALLMATVQSSLRTQLRHCLDSA
                     ANNGSAPVVVATAEFVAQLNQQLYADTAPEKYATFYFGVYDDTSSLLTYTNAGHLPPI
                     VIRREEVLRLDVNGIVVGAFPFAKYDESQIQLLSGDLLVCYTDGVTEPENEYGEMFGD
                     ERLIQVLQKNAGRDEEEIVSRVIDSVLEWTGAGELQDDMTLLVARRQ"
     gene            2301..3854
                     /locus_tag="VFQ79_00045"
     CDS             2301..3854
                     /locus_tag="VFQ79_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU0138062.1"
                     /translation="MKVLTAAQMREVDRRMIELGIPGIVLMENAGHRVVEFLAEKFSP
                     LSGHRIIVFCGKGNNGGDGFVVARQLFTRWKPAALHVVLTASPEELKGDAAENYRMLR
                     ACGCCPVSADLAPEMHSATLVIDALLGTGIKGSVSGAYLELIRAINSGFPAAKVVAVD
                     IPSGLASDSGECEGEAVKADYTVTFTALKVSQALPPACDRMGEVIVRQIGTPPDLYER
                     DDSIFLSQIHPAQFRHLLRARVPGGHKGTYGHVMVVGGSRGKSGAAAMTGMAAVRAGA
                     GLVTVASASSAIDVIAAHAPELMTEPLAETASGGISIQSYDDGRLSGLVRNKDVIAIG
                     PGLGGDSETVELVRQVFANCPHPMVVDADGLNALVGRDLQTKGRLRILTPHPGEMSRL
                     TGLATAAILKDRVDIARAFAQEHGVILVLKGQRTLLAFPDGRVWINPTGTPALATGGT
                     GDILTGIAAGFLAQFPDEPENAVAAAVYLHGLAGQIGERTLGDKSLAATDLLEYLPEA
                     MRECTRLPD"
     gene            3829..4281
                     /gene="tsaE"
                     /locus_tag="VFQ79_00050"
     CDS             3829..4281
                     /gene="tsaE"
                     /locus_tag="VFQ79_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU0138063.1"
                     /translation="MSAHVYRTESEEETIALGREIARSLPQSCVVLLIGNLGAGKTTL
                     AKGIVEGLGAASVEDVTSPTFTLIHEYSGNVYHVDLYRLDSAGQVAALGLEELLEGEA
                     VTLVEWGERFPEMMPDKRVEIRIARTGDDTREFRIDWLGLTPCPRTTP"
     gene            complement(4253..>5947)
                     /locus_tag="VFQ79_00055"
     CDS             complement(4253..>5947)
                     /locus_tag="VFQ79_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU0138064.1"
                     /translation="GYWTCTWREYLTAAEEIACGLRSLGIGKGDVVALDCETRAEFYL
                     ADVGVMSNGSIAAALYTTYPSADHVWYIRVSEARLIFLEDVAMLRRLTEETGGPLPAP
                     VVLLTGEAEGALTLAGLRARGRSAMTENPELMAAIERDVRASDTAILYLTSGATGEPK
                     MGMVSHQALVANIDMGPSVLPLSPEDSTIAFLPSAHIAQRVVVELLPIRCGAAVWFSA
                     GLSKLAGEFRKIRPTFFLAPPRVWERVHASISTEVRKRSAATRKLFHGALGLGLEAAR
                     LRNESKKVPQWLKVALKAADRLVFRKIRERFGGRLRVAASGAAPLGKDLADFYSAIGM
                     PLIEGYGLTEGGVAALNPLDRPKSGSIGKPLPGVEIRLTPDGELLIKSPCLFSGYFND
                     PATTAAVLKDGWLHTGDMAEIDAEGYIYITGRKKDLIVSSNGKKIYPARIEGVLKTEP
                     LINQVLLVGDRLPFVAALITVNVAAAADLKGMDQVRDKRPSHIATAPPVLEEIRKAVA
                     RANQKLAPFEQIRRYRILHRDFSIENGEVTPTMKVRRARVLENFRDEVKELYAGKE"
BASE COUNT         1137 a         1721 c         1865 g         1224 t
ORIGIN      
        1 gtgacggcga ttacgtgctg attgaaagag cttccgaaat tcacgacgga gagattgtcg
       61 tggccgtggt atcgaaatcc gaggccacgc tgaagcggtt ctatcgcgag cccaacggga
      121 tggcccggct gcaaccggcg aacgccgcta tggagcccat tgtcgttcct ttggaagatc
      181 tcgaaattca aggccggctg gtggcggttc tccggaaatt ccgctgacgc accggtgcgc
      241 cagccctaac gtacctccgt agcacaaggt attctcgaaa agatgacagg tagcagccga
      301 aacggctgga ttacgtcttt ataccggaag gtgggcatcg cggagaaagt ctttgttgtc
      361 ctcttcgtcc tttggcttgt ctttctcctc gcctcgcccg ccagcactat ccggcttttc
      421 ctccagttcg cgatgttcgc ggcggggatg gtggttctga tccggtatct gcgccgctgg
      481 gttcaaagcg cgatctggcg gttgcgcaac cggctctatg tggtctacct gtttatcgcg
      541 gttgtaccgg tttgtctgat cctgattctc ggcggcctcg cggcgtacct gattgccagc
      601 gaggtttcgg tttatctcgt cacctcggag ctgaaccggc gcgcggattc gctcggcaac
      661 ctggtcgagt ggcttgtccg tgcgcccgtg gcgcaacgct ctgcgtttct tcagaacgcc
      721 ggcaattacg ggcgtaaccg ctttcctggc ctcgaactta ttattcgcgg tagcggagga
      781 gggagctttc ccgcctccaa taaattagaa ccgcccccgg gtgattggcc ggaccagaac
      841 gggctggtgc tcaaggatga aatcctgtat gcctgggctc acatacggcg gaatgacgtg
      901 gacgtaacgg ccctggtccc gcttacgcag gattatctat ccgagttggt gcccaacctc
      961 ggggaagtga attacacctt ggcgccagcc gagaccggca atcctccggt cccaaactcc
     1021 ggtgtcctgt tccaggtgga tggcaccaac attcgcgctc cggcgcccca agcaggacga
     1081 aaaagccagt tgggtgttgc agtgaaccgg ttcgataaag aagtgctctg gttctctctg
     1141 atgcccgcgg cggtgtggga tgcgccgggc actacgcgga aggtcatcat cgccgtgcga
     1201 accaggttct ccgccgtccg gcaggcgatt tcgaccgaag caacttcgac cgagcgcgcc
     1261 ggtaccgatc agaacctgct gttcattttg ttcaccgtcg tcaccggcct gtttctcgga
     1321 gtcgagctga tcgcactggt tatcggcgta tctctgacgc gcaccatcac gggcgccgtt
     1381 cacaacctgt acgagggaac gcagaaagtg atgaagggtg agttctcaca ccggatcgag
     1441 gtgaagggga atgaccagct tgccgacctt ggaatttcgt tcaatcgcat gacggaaaac
     1501 ctggagcagc tcgtagtggt ggcgaaggag aaagagcgcc ttcaggctga gctggagctg
     1561 gcgcgcgaag tacaggccca gttatatccg aaagtaatgc ccacgctgaa gacactccgg
     1621 atcgtagcca cgtgcaatcc tgcgcgactg gtgtccgggg attatttcga ttaccagtgt
     1681 ctggaagata cgcaagtagc ccttgccctt ggcgatgtgg ctgggaaggg aatctccgcg
     1741 gccttgctga tggcgacggt gcagtcgtct ttgcgcacgc aactgcgtca ttgcctggat
     1801 agtgccgcca ataacggatc ggcccccgtt gtggtggcga ccgcggagtt tgtcgcgcag
     1861 ctcaaccagc agttgtatgc cgacaccgcg ccggagaaat acgcaacatt ctatttcggt
     1921 gtatacgacg ataccagcag tctgctgacc tacaccaatg cagggcacct gccccccatc
     1981 gtcatccgcc gggaagaagt cctccggctg gacgtgaacg gaattgtcgt aggtgcgttt
     2041 ccgtttgcca aatatgacga aagccagatc caattgctta gcggagacct actggtctgc
     2101 tataccgatg gagtcacgga accggagaac gaatatggcg agatgttcgg cgacgagagg
     2161 ctgatacagg tgcttcaaaa gaatgcggga cgggacgagg aggaaattgt ctccagggtg
     2221 atcgattccg ttctggagtg gaccggcgcg ggagagttac aggacgatat gacgctgctg
     2281 gtcgcgcgga ggcaataggg atgaaggttc ttaccgccgc acagatgcgc gaggtggatc
     2341 gccgcatgat cgaattgggt attcccggca ttgtgctgat ggagaacgcc gggcatcgcg
     2401 tagtcgaatt tctggcggag aagttttctc cattgtccgg tcaccggatt attgtgttct
     2461 gcggcaaagg aaacaacgga ggcgacgggt ttgttgtggc ccggcagctt ttcactcgat
     2521 ggaagccggc cgcgctccac gtggtgctta ccgcctcgcc cgaagagctg aaaggggacg
     2581 cggcggaaaa ctacaggatg ctgcgggcgt gcggctgctg ccccgtgtcc gccgatcttg
     2641 cgccggagat gcattcggct accctcgtca tcgacgcttt attaggcacc ggcatcaagg
     2701 ggtctgtttc aggcgcttac ctggagttga ttcgagcgat caacagcggc tttcccgctg
     2761 cgaaagtggt tgcggtggat atcccttccg gcctggcaag cgattccggt gaatgcgaag
     2821 gcgaagcggt gaaggcagac tataccgtga cgttcaccgc gctcaaggtg agccaggctc
     2881 tgccgccggc ctgcgaccgg atgggtgaag tcattgtccg gcagatcggc accccaccgg
     2941 acttgtatga gcgagacgac tcgattttcc tgtctcagat ccatccggcg caatttcgcc
     3001 atctgcttcg agcgcgcgtg ccgggtgggc acaaggggac ctacggacac gtgatggtgg
     3061 taggcggctc gcgaggaaag agcggggccg ccgcgatgac cggcatggcc gcggtgcgcg
     3121 ccggcgcggg actggtgacc gtggcgtcgg caagctccgc catcgatgtc atcgccgctc
     3181 acgcgccgga gttgatgacg gagccgctgg cggaaaccgc ttcgggcggg atctcgatcc
     3241 aaagctacga cgatgggcgc ctttccgggc tcgtccggaa caaggacgtc attgccatcg
     3301 gaccgggttt gggcggggac tctgaaaccg ttgaattggt ccggcaggtc ttcgccaatt
     3361 gcccgcatcc catggttgtc gatgccgacg ggttgaacgc gctggttggc cgggatcttc
     3421 agacgaaagg caggctgcgc atattgactc cgcatcccgg agaaatgtcc cgcctgaccg
     3481 gcctcgccac ggccgcaatt ctcaaagacc gtgtggatat tgcacgggcc ttcgcgcagg
     3541 agcatggcgt gatcctcgtg ttgaaaggac agcgtacgct actggcattc ccggatggcc
     3601 gggtgtggat caacccgacc ggaactcccg ctctggccac cggcggtacg ggcgacattc
     3661 tgaccggtat cgctgccggt ttcctggcgc agtttccgga tgagccggaa aacgcggttg
     3721 cggcggcggt ttaccttcac gggctggctg ggcagatcgg ggaacggact ctcggagaca
     3781 aatcgctggc cgcgacggac ctgctcgaat atctgccgga ggcgatgcgt gagtgcacac
     3841 gtctaccgga ctgaatccga ggaggaaaca atagcgctcg gccgtgagat tgcccggagc
     3901 ctgccgcaat cctgcgtggt attgcttatc ggtaatctcg gggcggggaa gacgacgctc
     3961 gccaaaggca tcgtcgaggg cttaggtgcc gcatccgtcg aggatgttac gagccctaca
     4021 ttcacgctga ttcacgagta ttccgggaac gtctatcacg tggacctcta ccggctggat
     4081 agcgcggggc aggtggcggc tctcggtctg gaggagttac tcgagggcga ggccgtcacg
     4141 ctggtggagt ggggtgagcg gtttccggag atgatgccgg ataagcgcgt cgaaatccga
     4201 atcgcccgca caggggacga cacgcgcgaa ttccgcatag attggctggg actcactcct
     4261 tgcccgcgta caactcctta acctcgtcgc ggaaattctc gagcacgcgc gcgcggcgga
     4321 ccttcatcgt aggtgtcacc tcgccatttt cgatggagaa gtcgcgatgg agtatccggt
     4381 accggcgaat ctgttcgaaa ggggcgagct tttggttcgc gcgcgccacg gctttgcgga
     4441 tctcttccag cacgggggga gcggtggcaa tgtgcgatgg ccgcttgtcc cgcacctggt
     4501 ccattccctt caagtccgcg gcggccgcga cattcaccgt gatgagcgcg gcgacaaacg
     4561 gcaggcgatc gcccaccaga agaacctggt tgatcagcgg ttccgtttta aggacgcctt
     4621 cgatgcgggc cgggtagatt ttcttaccgt ttgaggaaac gatgaggtct ttctttcgtc
     4681 ccgtgatgta aatatagcct tcggcgtcga tttcagccat gtcgccggtg tgcagccagc
     4741 catctttcag tacggctgcc gttgtggccg gatcgttgaa gtagcccgaa aaaagacacg
     4801 ggcttttgat cagcagttca ccatccggag taaggcggat ctcgacgccg ggcagaggct
     4861 ttccgatgct tccggattta ggacgatcga gagggtttaa ggcggcaacg ccgccttccg
     4921 tcagtccgta tccttcaatc agcggcatcc cgatggcgct gtagaaatcg gccagatctt
     4981 ttcctagagg cgccgcaccc gatgccgcca ctcgaagtct gccaccaaat cgctcccgta
     5041 ttttgcgaaa tacaagccgg tctgccgctt tgagggcgac ctttagccat tgcggcactt
     5101 tcttgctctc gttccgtagc cgcgccgctt cgagccccag gcccaaagcg ccgtgaaaca
     5161 acttgcgcgt agccgcagat ctcttgcgaa cctccgtgga gatgctcgcg tgcacccgct
     5221 cccagacacg cggtggagcc aggaagaagg tggggcggat cttccggaac tcgcccgcga
     5281 gtttcgatag tccagccgaa aaccataccg ccgcgccaca gcggatcggg agcagttcga
     5341 caaccacgcg ctgcgcgatg tgagcggaag gcagaaacgc aatcgtgctg tcctccggac
     5401 tgagcgggag caccgacggg cccatatcaa tgttcgccac cagcgcctgg tgcgacacca
     5461 tgcccatttt tggctcaccc gtggcaccgg atgtgaggta cagaatggcg gtgtcggagg
     5521 cccgcacgtc ccgctcgatg gcggccatca gttcggggtt ctccgtcatt gcactccgcc
     5581 cccgggcgcg taatcctgcg agcgtgagcg ctccctcggc ctctcctgtc agcagcacga
     5641 ccggcgcggg tagcggccct cctgtttcct ccgtaagcct ccggagcatt gccacgtctt
     5701 cgaggaagat cagcctggct tcggatacgc ggatatacca cacgtgatcg gcacttgggt
     5761 aggtggtgta cagcgccgcg gcaatggatc cgttcgacat cacacctacg tcggcaagat
     5821 agaactccgc gcgggtctcg caatcgagcg caacgacgtc gcccttaccg atgccgagcg
     5881 agcggagccc gcaagcaatt tcttccgccg ctgtcagata ttcccgccac gtacaagtcc
     5941 aatagcc
//
LOCUS       DASTMK010000003        11327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_480066, whole genome shotgun sequence.
ACCESSION   DASTMK010000003 DASTMK010000000
VERSION     DASTMK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11327
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480066"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..354
                     /locus_tag="VFQ79_00060"
     CDS             55..354
                     /locus_tag="VFQ79_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU0138065.1"
                     /translation="MAERMTQTQLVRELAESTELSNKTARALLDSLAEIAVRETKKNG
                     VFVIPGIGRLVRQERKARIGRNPATGESIKIPAKKVVKFRVAKSVKDAIVPPKKK"
     gene            complement(471..638)
                     /locus_tag="VFQ79_00065"
     CDS             complement(471..638)
                     /locus_tag="VFQ79_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU0138066.1"
                     /translation="MRSIGLPELLIILGIAVLLFGGRKIPEVAKGLGEGIKNFKNALK
                     TEDKTEEKKQA"
     gene            complement(719..1432)
                     /locus_tag="VFQ79_00070"
     CDS             complement(719..1432)
                     /locus_tag="VFQ79_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU0138067.1"
                     /translation="MQSIVDLFLEFYRSLTNPDKLISLLSTVLSGWWSYAALFLVVFA
                     ETGLLVGFFLPGDSLLFTIGVVAGAGGLDIVLINVVLMAAAIIGDGVGYSLGRRAGPH
                     IFNRPDSRFFRREHLQRTHEFYEKHGGKTIIYARFVPIVRTFAPFVAGVAGMSYWRFL
                     SFNVFGGIGWVALLTTAGYYLGSVPLVRRHFEKAIILVILLSIMPIVIEYLRARRRGK
                     TPARKVSPGLRPDTPSELG"
     gene            complement(1433..3361)
                     /locus_tag="VFQ79_00075"
     CDS             complement(1433..3361)
                     /locus_tag="VFQ79_00075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0138068.1"
                     /translation="MFDLFRRRDKAVRYLLTALLLVVALSMVTYLIPGSGMMGGPDDP
                     VLAEIGDEALTVHEVQRTLQTAMRSQQIPPQLVDVYVPEMINQMINERAVAYQARRMG
                     FRVTEEELAKAIQSTMPQLFENGQFVGRDVYAAVLAQQNLSISQFEDNFRKQLLMLKL
                     QNLVLEGVIVTPEEVEKTFRQRNEKVKVSYIALNPEKLRSQVTVTPAEVADYYEKNKP
                     AFQIPEKRSFDLIVIDEAQVAQSLGITDEELRKLYEQNQERYRVPERVRARHILLKTT
                     EKPKDEIPAIRAKAESLLKQIQSGGDFAKLAQENSEDPGSATKGGELGWITRGQTVKN
                     FEQSAFSLKPGQISGIIETEYGFHIIQVEEKEEPHVRPFAEVKNELAAESKRQQTYDK
                     MQTIADEVHAALVRSPEEGRQLAQKYGVPITHVSDADLSKPIEGIGNNPDFQEVIRSL
                     QKGQVTPIAQPAANKLAMAVLTDSKPSRQAELREVESEIRETVLKQKATELAKKRAAE
                     AEEKIQAAGGDLKKVAQSMGLEVKTTPDFTRDAAAEGIGPASYLAEAFEKEVGAVLPP
                     VTVGDQVFIVKVAGKTPADLTQLASQREDLVSSIRTAKARQRDELFKDGLLTQLIQDG
                     KVKIHDAAVKRLIASYRS"
     gene            complement(3479..4891)
                     /locus_tag="VFQ79_00080"
     CDS             complement(3479..4891)
                     /locus_tag="VFQ79_00080"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU0138069.1"
                     /translation="MKFGGTSMGSADRIRVAARILMEQQRQRPVAVVVSAMSKVTDLL
                     LESLLRAEAGDRAGLDANVQKLQDRHVECCHELLPKAKQEPVLAGIHFLISEFQRIAN
                     GMMMLADRPPRSADEAVAIGERLAALLMAACLEAENIRSVAINASDVIVTDAVFGNAT
                     PLMTPTREKARRNLVPWLEGGALPVITGFNGATADGRPTTLGRGGSDFSAAILAAVLD
                     ASELWIWTDVDGIMTADPRIVPDAAVLDTVSYAEAAELAYNGAKVLHPRTLAPLADRQ
                     IPVWSKNSYALDKPGTRIGMPVNEPGGARAVTSMSNVALVSLEAAGPELSGTRLMARA
                     LDALTRANAEVLAVSSSSYRQNFCFLVRRDELSGVMKSLEETLSIELAHGYIRPIQVD
                     ENVGLLAVVGEGMRGVAGLAGRIFTAISREQVNIIAIAQGSSELTICIVVRRDGLEHA
                     VRAVHTECGLGHRVRETAVP"
     gene            complement(5314..6003)
                     /gene="cmk"
                     /locus_tag="VFQ79_00085"
     CDS             complement(5314..6003)
                     /gene="cmk"
                     /locus_tag="VFQ79_00085"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU0138070.1"
                     /translation="MSTRIVVAIDGPAGAGKSTIARRVAERLGFIYIDTGAMYRAVAL
                     WALRTGVDLADMHRLEQLAIAASIELPPRSAAVLLNGEDVTAAIREPAISPAASKVSA
                     VLGVRRALVDKQRQMAAENSVVMEGRDIGTVVFPEAKVKIFLDADPETRTERRLKDLQ
                     QRGTTLAPEAVAQEVQERDQRDRTRAESPLVQAPDAVYIDSTGLTIEQVEELILKIVR
                     DRTSNGKGLRP"
     gene            6067..6414
                     /locus_tag="VFQ79_00090"
     CDS             6067..6414
                     /locus_tag="VFQ79_00090"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEU0138071.1"
                     /translation="MSPAEFDTLVERALKKIPARFRKRLRNIVFVVEPEPPRPNLLGL
                     YHGRPLTNRSVYDSFHLPDQITIYQGPHERIAKSRAELEKLVEETVWHEVAHYFGMDE
                     QRVRRAERRRRAR"
     gene            complement(6487..6663)
                     /locus_tag="VFQ79_00095"
     CDS             complement(6487..6663)
                     /locus_tag="VFQ79_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138072.1"
                     /translation="MRQLLFLIISIAMLGTSTLMLGQSVAADKSGRVASEAASGVSHF
                     SRGMGSDPIIIVIP"
     gene            complement(6783..9395)
                     /locus_tag="VFQ79_00100"
     CDS             complement(6783..9395)
                     /locus_tag="VFQ79_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138073.1"
                     /translation="MWGKRTPRDFRAEIQAHLELEADQLRADGIPPDEALAAAHRAFG
                     NRTIAEERFYETARVMWLEHLIQDLRFGARLMWKNPVFAATIILVLALGTGANTAVFS
                     VVNAVLIRAVPYDRPEELFRLFQDQGSGRMPVAPANYLDWRAQSRSFRHMAAFQAEVF
                     RLDRGGEPEKFAGARVSSGLFATLGVGAALGRVFAPEEDKPGGPKTVVLSHSLWTTAF
                     AADRQVINRQIRLDGQSVTVIGVMPRGFAFPSEHSVLWTPLALGDTGRSLSRTENYLG
                     VIGRLKDGITAREAQAELDTIARRLAAAYPNSNERLGVALRSLREYTVGRVSTALLVL
                     LAAVAFVLLIAGANVSNLLLAKTAARRREIAVRVSLGASRSRVIRQLLAESLLLGLAG
                     GAAGWLLAQVSFGLLLQLIPDSVPGASRIRLDGQVFFFTLAISLIAGVLFGLAPALDA
                     TRSKFTESLQQGGRTSAGRRAARLRDVLVAGEVALAFVLLAGCGLTVRTMMKLYDVDP
                     GFNPDHLITVPVTFSLPPEKEEALEQPEARAAIAFRELIARAAALPGVREAGAVSAVP
                     LTQEYTGTRFMLEGRSVPTTQRVPEADYSVVTPGYFKAMEIPLRRGRMFTDSDGKSTP
                     PVAVINEAFARRFFPGEDPVGRRLRRGGRDSDRPWITIVGVAGNIRHLSLDTPPVPEL
                     FFPHAQAAWPQLHLVIRTNVDPVSLIEPLRRLVREMGSNHDVGTPRTAEQLLASSVGT
                     RRFAMQLLSLFALLAFVLAGTGIYGVLSWGVAQRTREIGIRMALGASASRIVRLVLAQ
                     GTAPVAVGILAGLAGCFAVTHVMANLLYEVSPYDAATFVVALALLILVAVAALFGPAR
                     RASRTDPLAALRHD"
     gene            complement(9401..9757)
                     /locus_tag="VFQ79_00105"
     CDS             complement(9401..9757)
                     /locus_tag="VFQ79_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0138074.1"
                     /translation="MATREPDEDRIALLQGTLDLLILRTLVFGAEHGQGIARAIQQQS
                     QNVLLVDHGTLYPALQRLEAKGWIEAKWGVSANNRKARFYSLTKAGRKQLVQETSQWR
                     KLAAAIGRILGPEAAE"
     gene            complement(9815..>11327)
                     /locus_tag="VFQ79_00110"
     CDS             complement(9815..>11327)
                     /locus_tag="VFQ79_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138075.1"
                     /translation="TVCAGLNLEDTVCEGNVDLSGIYVCERGAAQKQIYEAPFAVHNG
                     KVFLPIAFDSKPGEVIAIAGKAESPLEWEPQERDGIRREMALDVADLRGVDSVEIRFA
                     NSKQAMPEFPDVAARSFNVAGMLTMWCRYDGIGVRSACVEGRLDLSNASASEAKFCGD
                     SFDREGWGRRVQGCGNKNELHRFRPWLQFERAKFGRFSLCEPFPFSVDFGQMEVGQWD
                     FFSADGSKTNEDYLTLLSFSDPFRRSTYLDLIRTLRDQGRDDEVVKLWDAMHDREFKR
                     GWGLVDGEYQACRQLAAPGSHGRSLLARAWHKFKKEQTHFYANTWPLFRIWLVLLAAS
                     VVVFWQAGNIVPSPEQLGENVPDPSTAFPLRYTPYVQGSGEFAESHPADWGLVQAASL
                     ALRYHVPPIQLFLDRRWEPASNGPMMIPNPLRLLAAAPALERGIGCEAGFQQTERYAK
                     TNGSPTHCSTHFAFGWPWPSPLTWATVMTLLNWLMVPALLYGFFARRFRSPWS"
BASE COUNT         2365 a         3502 c         3293 g         2167 t
ORIGIN      
        1 gttaggatac ctgtcacatt cattgggaga atcgacaaac agtaagggag atttatggcc
       61 gagagaatga cgcaaacgca actcgtgaga gaactcgccg aaagcacgga gctaagtaac
      121 aaaaccgccc gtgctcttct cgacagtctc gctgaaatcg ctgtacgcga aacgaaaaag
      181 aatggtgttt ttgtgatccc cggcatcggg cggctggtgc ggcaggagcg gaaagcccgc
      241 attggcagaa acccggcgac gggcgaaagc atcaagattc ccgctaagaa agtggtgaag
      301 ttccgggtgg cgaaatccgt gaaggatgcg atcgtaccac cgaagaagaa gtaagaccgg
      361 gcctcaaaaa aagttcagac acgaaaaccc ccgcgtaatg gcgggggttt gttccatcca
      421 taaagccgag ttgagtctcc gctcaaaaac gtgcgagcgg gccaggaagc ctacgcctgc
      481 ttcttctctt cggtcttatc ctcagttttc agggcattct tgaaattttt gatgccctct
      541 cctaaaccct tcgccacttc ggggattttc cgtccgccga agagcaggac ggcgattccc
      601 agaatgatga gaagttcagg caagccaata gacctcatgc ggcctccttc gtgttgctaa
      661 catctccatt gtaacggtcc gggcaaaccc gaggcaagaa cggccttttc tgttcgtgtt
      721 agccgagctc cgaaggcgta tccggccgga ggccgggtga aactttccgg gccggcgtct
      781 ttccgcgacg gcgggcacgc aaatactcga tcacgatggg catgatggaa agcagaatga
      841 cgaggataat ggctttctcg aaatgccgtc tgaccagcgg cacgctgccc agatagtatc
      901 cggccgtcgt caggagagcc acccacccga tgccgccgaa gacgttgaag gacagaaagc
      961 gccagtagct catgcccgcc acgcccgcca cgaacggcgc gaaagtcctc acaattggaa
     1021 cgaaacgggc gtagatgatc gtctttccgc cgtgcttctc gtagaactcg tgcgtgcgtt
     1081 gaaggtgttc ccgccgaaag aagcgcgaat cgggccggtt gaaaatgtgc ggccccgcgc
     1141 ggcggcccag cgaatagccg acaccatcac cgatgatggc ggcggccatc agtacgacat
     1201 tgataagaac aatgtccagc ccgcccgcac cggccaccac gccgatcgta aacagcaggg
     1261 agtctccggg cagaaagaag ccaaccagca gccccgtttc ggcaaagacc acaaggaaga
     1321 gcgccgcata gctccaccac ccgctcaaca cggtggaaag cagcgaaatc agcttgtccg
     1381 gattggtgag gctgcggtaa aactcgagaa aaagatcgac tatactttgc atctaactcc
     1441 gatagctcgc aatcaggcgt ttcaccgcag cgtcatggat tttcactttt ccgtcctgga
     1501 ttaattgcgt gagcaagcca tctttgaaca actcgtcccg ctgccgcgct ttcgctgtcc
     1561 tgatggacga aacaagatct tcccgttgcg acgcgagttg tgtcagatct gccggcgtct
     1621 tcccggcgac ttttacgatg aatacctggt ccccgaccgt aaccggaggc agcacggcgc
     1681 caacctcttt ttcgaacgct tcggcgagat aacttgccgg gccgatcccc tcggccgccg
     1741 catcccgcgt aaagtcgggt gtggttttaa cctccagacc catcgattgc gctacttttt
     1801 tgagatcgcc gccggcggct tggatcttct cctccgcctc ggcggctctc tttttcgcca
     1861 actctgttgc cttttgcttg aggaccgttt cgcgaatctc gctctccacc tcgcgaagct
     1921 cggcctgccg ggaaggcttg ctatcggtaa gcacagccat ggccaacttg ttcgcggcgg
     1981 gctgtgcaat cggtgtaacc tgccccttct gaagcgagcg gattacttcc tgaaaatcgg
     2041 gattatttcc gatcccttcg atgggcttgg acagatccgc atcggagaca tgagtaatgg
     2101 gcacgccgta tttctgcgcg agctgccgcc cttcctcggg cgagcgaacg agagcggcat
     2161 gcacctcatc cgcgatagtc tgcattttgt cgtaagtctg ctgccgcttc gattccgccg
     2221 caagctcatt cttgacttcc gcgaacgggc ggacgtgggg ttcttccttc tcctccacct
     2281 ggatgatatg aaacccatac tcggtttcga taatgccgct gatctgtccc ggcttaagag
     2341 agaatgcgct ctgttcgaaa ttcttcacgg tctgcccgcg ggtaatccag ccgagttctc
     2401 ctcccttggt ggccgagccc gggtcttccg agttctcctg cgcgagcttc gcgaaatcgc
     2461 cgccggattg gatctgcttc aacagggatt cggctttcgc ccggatcgcc ggtatttcgt
     2521 ctttcggctt ctcggtggtc ttcaacagaa tgtgccgggc cctcacccgt tccggcacgc
     2581 ggtagcgctc ctggttctgc tcatataact tccgcaactc ttcgtcggtg atcccgagcg
     2641 actgcgcaac ctgggcttcg tcgataacaa tcagatcgaa gctgcgtttt tcgggaatct
     2701 ggaacgccgg cttgttcttt tcgtaataat ccgcgacctc cgcgggtgtt accgttacct
     2761 gggaccggag cttctccggg ttcagcgcaa tatagctgac cttcaccttc tcgttgcgct
     2821 gccggaaggt cttctccacc tcctccggcg tcacgatcac accttcgaga accaggttct
     2881 gaagcttcaa cattaagagc tgcttgcgga aattatcctc gaattgcgag atggagagat
     2941 tctgctgcgc gaggaccgcg gcgtaaacgt cgcgtccgac gaactgcccg ttttcgaaga
     3001 gttggggcat cgtcgattga atcgccttgg ccagttcttc ctccgtgacg cggaagccca
     3061 tccgcctggc ctggtaagcc accgcccgct cgttgatcat ctgattgatc atctccggaa
     3121 cgtagacatc cacgagttgg ggcgggatct gctggctccg catagccgtc tgcaacgtcc
     3181 tttggacttc atggaccgtc aacgcctcat cgccgatttc ggccagcacg ggatcatctg
     3241 gtcctcccat catcccggaa ccgggtatga ggtaggtgac catggaaagc gcgacaacta
     3301 acagcagcgc ggtaagaagg taccgcacgg ccttgtccct tctgcggaac agatcgaaca
     3361 tgaaacaact ctcctaatcg taacggcttc gtcggaatcg tacgaaccgc ttcttcggga
     3421 agcggtcagg ggaatagtgc gcttgtatag acgaagatct agatcatagt ataacgggtt
     3481 acggaacggc agtttcacgc acacggtgtc ccaacccgca ctcggtatgg accgcccgga
     3541 cggcgtgttc cagaccgtcg cggcgaacca cgatgcaaat cgtcaattcg ctggagccct
     3601 gggcgatcgc gataatgttt acctgctccc gcgagatggc cgtaaagatg cggcccgcca
     3661 gtccggcaac tcctcgcatt ccttctccga cgaccgccag gaggccgaca ttttcatcca
     3721 cttgaatagg ccggatatag ccatgcgcca actcgatgga gagcgtctct tcgagcgatt
     3781 tcatcacccc gctcagctca tcgcgccgga ccagaaaaca aaagttctgg cgatagcttg
     3841 agctggaaac agcaagaacc tccgcgttcg ctctggtcaa agcatcgagg gcacgcgcca
     3901 tcagccgtgt gccgctcaat tccgggccgg cggcttcaag tgaaaccagg gcgacgttcg
     3961 acatggaggt taccgcgcgg gctccaccgg gctcatttac cggcattcca atgcgcgtac
     4021 ccggcttgtc cagcgcgtaa ctgttcttgc tccaaaccgg gatttgccgg tcggctaaag
     4081 gagccagagt tcgagggtga agcaccttgg cgccattgta agcaagttcg gcggcttcgg
     4141 cgtaagaaac cgtgtccagc acggctgcat ccgggacgat gcgggggtcc gccgtcatga
     4201 tgccgtccac gtccgtccag atccacaact cagaagcgtc aaggaccgcc gccagaatag
     4261 cggcggaaaa atcgctgccg ccccttccga gggtggtcgg acgcccgtcg gccgtggccc
     4321 cgttaaagcc ggtgattaca ggcagggcgc cgccttcgag ccacggcaca aggttccggc
     4381 gtgccttctc gcgcgtcgga gtcatcagtg gagtggcatt tccgaacacc gcatcggtga
     4441 caatgacgtc ggacgcgtta atggccaccg agcggatgtt ctccgcttcc aggcaagccg
     4501 ccatcaaaag cgcggcgaga cgctcaccga tggcgaccgc ttcgtccgcg gaccgcggcg
     4561 ggcggtccgc aagcatcatc atgccgttgg cgatgcgctg aaactccgaa atcaggaaat
     4621 gaatgccggc cagcaccggc tcctgcttcg ccttgggcag caactcatgg cagcattcca
     4681 cgtgccggtc ctggagcttc tggacgttgg cgtcgagccc ggcccggtct ccggcttccg
     4741 cgcggaggag cgattccagc agcagatcgg ttaccttcga catggccgac accacaaccg
     4801 ccaccggcct ttgccgttgc tgctccatca agatccgggc cgcaacgcgg atccggtcgg
     4861 cggaacccat actggttccg ccgaatttca tgatcaggag atccttcaaa ctcacactgc
     4921 ctcgtctgtt cgtaatctct ccggtcccta cgaaaaccgc tcagtatcga gcgaatgttt
     4981 ccgaaccgcg accgcaagga agcggacatc agaaaacttc tgttcggaat tacggtgacc
     5041 aaccggatac cgcttgctgg gaagctgtga agaaattgaa tttagccacg gatgaacacg
     5101 gatgaacaca gataagtttc ctgttttcaa tctgtgttta tctgtgtcca tctgtggctc
     5161 tgaatccttc acaccttctg aggcgcgcgg atacgtttcc gagccgtgac cgtgagggag
     5221 cggccccttt cgaaatacgt accgaatttc tgaagccatc tacttagaat aatcggtcgc
     5281 gaaactcgcg gggggcgcag cggggcaccg ttttcaaggc cgaagccctt tcccgttcga
     5341 ggtccggtcg cggacgatct tcagaatgag ctcctccacc tgctcgatcg tgaggccggt
     5401 ggaatcgata taaacggcat cgggcgcctg aacgagggga gactccgccc tggtccggtc
     5461 gcgctggtcg cgctcctgca cctcttgtgc cacggcttcc ggcgccagcg tggtaccgcg
     5521 ctgctggagg tctttcaacc ggcgctcggt gcgggtttcc gggtcggcgt cgagaaagat
     5581 tttcactttc gcttccggga atacgaccgt gccgatatcg cggccctcca tcaccacgga
     5641 gttttcagcc gccatttgcc gctgcttgtc gaccaaagcg cgccggactc ccagaactgc
     5701 ggagaccttc gaagcggcgg gcgagatggc gggttcgcgg atggccgcgg tcacgtcctc
     5761 gccgttcagc aggacagcgg ccgagcgggg cggcagttcg atgctggcgg caatcgcaag
     5821 ctgctccagc cggtgcatgt cggccagatc cacgcccgtg cgcagtgccc acagcgcgac
     5881 cgcacggtac atggcgcccg tgtcgatata aataaacccg agtctttcgg cgaccctgcg
     5941 ggcaatcgtg ctcttgccgg cgcccgccgg tccgtcaatg gcaaccacaa ttctggtaga
     6001 catgtcgatg ctcatccggc gttccgggac tgtgccctca cccggtcctc tacaatagag
     6061 cgacggatgt ctccagccga gtttgacacc cttgtggaac gggctctcaa aaagataccg
     6121 gcgcgctttc gcaagcggct ccgcaatatc gtttttgtgg tcgaacccga accgccgcgg
     6181 ccgaaccttc ttggtttgta ccacggccgc cccttaacca accggagcgt gtacgattcc
     6241 ttccacctgc ccgaccagat caccatctac cagggaccgc acgagcgcat cgcgaaatcc
     6301 cgggccgagc tggagaaact cgtcgaagaa acagtatggc atgaggtggc acactacttc
     6361 ggaatggacg agcagcgtgt acggcgcgcc gaacggcggc ggcgtgcgcg atgaccgccg
     6421 gcggggtccc gcgcggttca gaatgcggcc ggatcgcggc ggcactcgaa caaaggcgat
     6481 gagttgctac ggaataacga tgataatggg gtcggaaccc attccccgcg agaagtgcga
     6541 gacgccgctc gccgcttcag aagcgacccg cccgctcttg tcagccgcaa cggactgccc
     6601 gagcatgagc gtggaagtac ccaacatggc gatggagatg atcaggaaga gtagttgacg
     6661 catacctgca cttccatcgg caggcatagg tgagaactgt aggagggaat acggtgtttc
     6721 tgtcaggcga catcaggcgc cggagaaatg tttctccggt aattcgagga ttccaggacg
     6781 ggctaatcgt gccgtaaagc ggccagcgga tcggtccgcg aagcacggcg tgccggccca
     6841 aacaaggctg cgaccgccac gagaatcaac aacgccaggg ccacaacgaa ggtggcggca
     6901 tcgtatggag aaacctcgta aagaagattc gccatcacgt gcgtgacagc gaagcaaccc
     6961 gccagacccg ccagtatccc gacggccacc ggagcagtac cttgagcaag gaccaggcga
     7021 acgattcgag aagcgctcgc gccgagagcc atgcgtatgc cgatctcccg ggtacgctgg
     7081 gcaacgcccc aggagagcac accgtaaatc ccggtaccgg ccagcacgaa cgccagcaga
     7141 gcaaacaatg aaaggagctg catggcgaaa cgtcgcgtac ccaccgatga agccagcagt
     7201 tgctccgcgg tccgtggggt accgacatcg tgattcgatc ccatttcgcg caccaaccgc
     7261 cgcaaaggct cgataaggct cacgggatcg acgttcgtgc gaatcaccag gtggagttgg
     7321 ggccaggcgg cctgggcatg tggaaagaac aactcgggga cgggcggtgt gtccagactc
     7381 agatggcgga tgttaccggc aacccccacg atggtgatcc aggggcgatc ggaatcgcgg
     7441 cccccacgtc tcagacgacg gccgacgggg tcctcgcccg ggaagaatcg ccgagcaaaa
     7501 gcttcgttga tgacagccac aggcggcgtg ctttttccgt cggaatccgt aaacatacga
     7561 ccacgacgca gcggaatctc catcgccttg aaataaccgg gcgtgaccac gctgtaatcg
     7621 gcttccggaa cgcgctgtgt ggtgggaacg gagcggccct ccagcatgaa ccgcgtgccg
     7681 gtatattcct gggtcagcgg cacggcacta acggcgccgg cttcgcgtac tccgggaagc
     7741 gcagccgcac gggctatcag ctcccggaag gcaatagccg cacgtgcctc cggctgctcc
     7801 agcgcttcct ccttttccgg aggcaacgag aaagtgaccg gcacggtaat caaatgatcg
     7861 ggattgaaac cagggtcgac atcgtacagc ttcatcatgg ttcgcacggt caggccgcat
     7921 ccggcaagca gaacgaaggc gagcgccacc tccccggcaa ccaatacgtc acggaggcgc
     7981 gccgcccgcc tgccggctga tgtgcgccca ccctgctgca agctctcagt gaacttgcta
     8041 cgcgttgcat cgagtgccgg cgcaagtccg aagaggactc cggcgattag cgagatagcc
     8101 agggtaaaga aaaatacctg gccgtcgagt cgaattcggc tggctcccgg gacgctgtcg
     8161 gggatcaatt ggagcaggag tccaaagctc acctgcgcga gcaaccagcc cgcggctccc
     8221 cccgccaggc cgagtagcag actttccgcc aacaattgcc tgatgacacg cgagcggctg
     8281 gcgcccagtg agactcgaac ggcgatctcc cgccggcggg cggccgtctt tgccagcagc
     8341 agattcgaca cattcgctcc cgcaatgagc aacacgaaag ccacagccgc cagcagaacg
     8401 agcaatgccg tgctcacccg cccaaccgtg tattcgcgaa gcgaacgaag agccacgccc
     8461 agccgttcat tcgaattcgg ataggccgca gccagccgcc tggcaatggt gtccaactcc
     8521 gcctgggctt cgcgcgctgt aatcccgtct ttcaatcgcc cgatcacgcc aaggtaattc
     8581 tctgtccgcg agagggagcg gccggtatcg cccaaagcaa gcggggtcca aagcacagag
     8641 tgctcggacg gaaaagcaaa accgcgaggc atcacgccga tgacggttac gctctgtccg
     8701 tcgaggcgta tctgccggtt gatcacttgc cggtcggccg caaatgcggt tgtccaaagc
     8761 gaatggctga gcaccacagt cttcggcccg cccggcttgt cttcctccgg agcgaatacc
     8821 cggcccagcg cggcaccgac accaagcgtt gcgaagagac cggaggaaac gcgtgcgcca
     8881 gcaaacttct cgggctcgcc accacgatcc aatcgaaaca cctccgcctg aaacgccgcc
     8941 atgtggcgga acgaacggga ttgtgcgcgc cagtcgagat agttcgcggg tgcgaccggc
     9001 attcgcccgc tgccctggtc ctggaacagc cgaaacaact cctccggccg atcgtacgga
     9061 acggcgcgta tcagcacggc gttcaccacg ctgaagacgg ccgtgttggc gccggttccc
     9121 agagccagca ccagaatgat cgtcgcggcg aataccggat tcttccacat caggcgagcg
     9181 ccgaaccgaa gatcctgaat caggtgctcc agccacatca ctctggctgt ttcatagaac
     9241 cgttcttctg cgatggtgcg atttccgaaa gcccggtgtg cggctgctaa tgcctcatca
     9301 ggaggtattc cgtccgcacg gagctggtcg gcttccaatt cgagatgagc ctggatctcc
     9361 gcccgaaaat cccggggtgt gcgcttcccc cacatggcag ctattccgcg gcttcaggtc
     9421 ccagaatccg cccaatcgca gccgcgagtt tcctccattg gctggtttcc tgtaccaact
     9481 gcttccggcc ggcttttgtc aggctgtaga accgcgcttt gcgattgttc gccgagacgc
     9541 cccacttcgc ttcaatccag cccttcgctt cgaggcgctg caaagcggga tacagcgttc
     9601 cgtgatcgac gaggagaaca ttctgcgact gttgttgtat ggcgcgggcg atgccctgtc
     9661 cgtgctccgc cccgaaaacg agcgtgcgca gaattaacag gtccagcgtg ccctgaagca
     9721 gcgctatgcg atcctcatcg ggttctcggg tggccattcg acccaagtat gcgccgattc
     9781 gggtggcatg tcaacccctc attggttgag ccggtcaact ccagggcgaa cggaaccggc
     9841 gggcgaagaa gccgtacaga agcgccggga ccattaacca gttcagcagt gtcatgacgg
     9901 tggcccaggt cagcggcgat ggccagggcc atccgaaggc aaaatgcgtg gagcagtgcg
     9961 ttgggctgcc gttcgtcttc gcataccgct ccgtttgctg aaagcccgcc tcgcatccga
    10021 tgccccgctc cagggccggg gcagcggcga ggagccgcaa gggattcgga atcatcatgg
    10081 ggccattgga ggcgggttcc cagcggcggt cgaggaataa ctggatgggc gggacgtggt
    10141 agcgcagcgc cagcgaggcc gcttgaacga ggccccaatc cgccggatgg ctttcggcaa
    10201 actctcccga tccctgcacg tagggcgtgt accgcagcgg aaaggcagtg ctcgggtcgg
    10261 gaacattttc gccgagctgc tcgggcgacg gcacgatgtt tccagcctgc cagaaaacga
    10321 cgaccgaagc cgccagcaag acaagccaaa tgcgaaacag cggccaggta ttcgcgtaaa
    10381 aatgagtctg ctcttttttg aatttatgcc acgcgcgggc cagcaggctg cggccgtgcg
    10441 aacccggggc ggcgagttgc cggcacgcct ggtactcgcc gtccaccaat ccccagccgc
    10501 gtttgaactc acggtcgtgc atggcatccc aaagcttcac cacctcgtcg tcgcggccct
    10561 gatcgcggag cgttcgaatc aaatcgaggt aagtgctgcg gcgaaacggg tcggagaagg
    10621 acagaagagt cagatagtct tcgttcgtct ttgaaccgtc cgcggaaaag aagtcccact
    10681 ggccaacctc catctgcccg aaatcgacgg aaaacgggaa aggctcacag aggctgaacc
    10741 ggccgaattt cgcccgctcg aactggagcc agggtctgaa acgatgaagt tcgtttttgt
    10801 tgccgcaacc ctggacgcgg cgaccccagc cctcgcgatc gaagctgtcg ccgcagaatt
    10861 tcgcctcgct ggctgaggcg ttcgacaggt ccagccgtcc ctcgacgcag gcggatcgca
    10921 ccccaattcc gtcgtatcgg caccacatgg taagcatgcc ggcgacattg aaactgcgcg
    10981 cggccacatc cggaaattcc ggcatggcct gcttggagtt tgcgaatcga atttcgacgc
    11041 tatccacacc ccgaaggtcc gcgacatcga gcgccatttc tcttcgaatg ccgtctcttt
    11101 cctgcggttc ccactccaaa ggggattccg ctttcccggc gattgcgatc acttcacctg
    11161 gtttcgagtc gaatgcgatt ggaaggaaga ccttgccatt atggacggcg aacggcgcct
    11221 cgtaaatctg tttttgggcg gcgccccgct cgcagacgta aatgcccgaa aggtccacgt
    11281 tgccctcgca cacggtatct tccagattga gacccgcgca gaccgtg
//
LOCUS       DASTMK010000004        10406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_576070, whole genome shotgun sequence.
ACCESSION   DASTMK010000004 DASTMK010000000
VERSION     DASTMK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576070"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="VFQ79_00115"
     CDS             <1..1857
                     /locus_tag="VFQ79_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0138076.1"
                     /translation="ETGKQIWHFEADKVALRGLAWWPGDRNTHPRVFAGVQGGMIAVD
                     AKTGRPATGFANEGLLDLKQGVLGDLPDARISLQSPPTVYKNIVITGSTNGEGAPTKG
                     AYGDIRGWDAYTGKLVWSFHTVPRPGEPGNETWPPGGWKNRSGANAWGFLALDVKRGI
                     VYVPLGAPTSDFYGADRHGDGLYGNSLVALDAATGKLKWYQQLVHHDLWDFDLAAAPT
                     LFDASLNGRVVPAVAQITKMGILFVFDRVTGKPLYGMEERPVPQSTVPGEKTAATQPF
                     PLKPPPLSRIDFTKEEIYNLTPEHAAFCQDLFESNQMRIGSLYTPLGLEGNVLMFPST
                     LGGGGWGGVSIDPSLGYLFTNVNNLGQWGHMEKRIDRSTGAETYVRTSAHGTYARFWN
                     RETRIPCSNPPFGELVAVDTRTGDIAWRTPLGTVPALEEKGVKNTGTLNLGGSIATAG
                     GLVFIGATNDSRFRAFESKTGRLLWEQPIDANGHTVPITYLGKDGKQYVTIMAGGGGG
                     YFGGTPSDSVVAFALGEGSAPPVVTKTSPAPQRESPVRAAGPTTLPDGAGKAAVLRSC
                     AGACHDMEVVVGVRRDRAAWAAMVDNMIARGAKAKGDEVKLIIDYLTTHFGK"
     gene            complement(2341..5895)
                     /locus_tag="VFQ79_00120"
     CDS             complement(2341..5895)
                     /locus_tag="VFQ79_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138077.1"
                     /translation="MNALDQLNQYLRRLESRLKFAAVSRGAAIAAIAALAATVLLVLL
                     ANRFAFSETSVTGARVLLFLALAFAIAFGLVLPLLKLNRRAAARRAEQQFPEFEQRLL
                     TLAEKNREEPDPFLELLAANTVEVARTAEPERVAPRTWLVGFLSTAAAAVIALVWLAV
                     SGPGFLGHGTALLWGGTPKAGSQPFYDIRVSPGDAKIRRGSDQLVSANLMGFQSPRVR
                     LFARFHSSTKWEETPMQVEAGGSGYEFLLAGISENIEYYVEAGGIRSKHFTLGVVDLP
                     AIQSMRITYNYPAWLNLKSVVDESTGDLRAVEGTEAEVAIQFDRPLAQGALIVDNENR
                     LALNGTGQNWYSARLKMSKDAVYHVAAIEHDAPVRLSEDFFISIENETPPLVRISRPG
                     RDARVSPIEEVPVAVTATDDFGLHAMELRYAVNGGPEKVVPLLKNKGAKEADGSTLIA
                     LEDFKLVPGDLVSLYAVARDARSNSQTDIFFIEAQPFEREYSQSQQMGGGEGEGEQQD
                     NNISQRQKEIIAATWNELRAEKKSNTHAAENAKFLSEVQGKLRDQARSLAKRMRSRQL
                     SGTNQEFQSFSKDMEAAAEAMNSAVDQLKSQAWRGALAPEQKALQHLLRAESTFRQIQ
                     VAFGSRGGGGGGQGGRDLENLFDLELDTEKNQYETGQRSASTEQRSREIDEALQKLEQ
                     LAKRQQELAQQQRNQSQSFQQRWQQEMLRREAEELKRRMQELAQNSGSQQSGQQSSQQ
                     SQQSGQQGQSSSSGQQGRMQRGQSGGDPRLERALERLEQATRDMRNAGSQQQNSADSR
                     RAAERLQEARDALGGMRRQQASGQLDDLSQRAGELASQQRNFADKLRQMYGREQGQGL
                     RPGTSRQDVERLADEKRNMLSELQRLERDMKDSVRDLAGTERTASSKLREALGDMQQN
                     ELGLRMKFQSEYIRRGLGAYAWMREQTITQGLDRLSDQVKEAQRALGESKSGDQENPG
                     LKRSLAQVEELRNRLQRQMSQLSRGQQGQQDQQGQQGQQGQQGQGGQQGQPGQQGAQG
                     QRGADGQGRQGNPGGLERGDGYTAMNRGDLPGPIGRAADPSAVRGLERAYSEGLRDLS
                     ELRRDLQAGAPEMDRDVQQLIREMQQLDPKRFPGNPALLEKIHGQVLPALEQLELQLR
                     RQVDGGQSDQVLTGASEKVPTGYADAVAEYFRRLSKTR"
     gene            complement(5892..7919)
                     /locus_tag="VFQ79_00125"
     CDS             complement(5892..7919)
                     /locus_tag="VFQ79_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEU0138078.1"
                     /translation="MGFLSPWFLAGVAAIGLPVWLHLLKQHKTTPFRFSSLMFFERRT
                     QSSVKHRRLKYLTLFAMRVALLLLLILAFAHPYIMRAVPAGGGGRKLLVVAIDNSFSM
                     RESNRLARAKQEAKAALAELRSGDEAQVLAFGSGVRLMTQPTNDKGQLVSAIDEIEPG
                     DTRSSYAELARALRSIGASSRAPVEAHVFTDIQKSSLPPSFADLQVADGTKLSLHSLA
                     PARVPNFAVENVTAPARVYNTRQVRIQATIAGYGAAAARRSVSLVLNGKEVERKEASV
                     PAEGRATVEFHGLEAPHGFNRGEVRIDSADTLAADDTFFFAIERAEPSRALFVHEARQ
                     PRGLLYFREALDSASNSAFILDALTAAQINNADFSKYRFVVLSDAASVSSASEEALKA
                     YLQQGGSVLIAAGPALGTRARIPVTGDQVIASRYSSRAAERFQSVASIDTSHPSLQSA
                     GQLDGVKFYQTVEIAPGDSRVLIRLADQRPLLLEKTIGTGRVLTFASTFDNISNDFPL
                     HAAFVPFIEQTARYLAGHAGRSAGYLVDAHLELRGNRDDGVAFEVLDPRGQRALTLAE
                     AAKVNSLQLSEAGFYQINRANGQREMVAVNADRNESDLDIITGESLALWENTGRPAEA
                     AAAATGASEEKRWDLWWFVMLAVLLTAIAESMFGSRYLAGEAPEKVQGGIA"
     gene            complement(7920..8807)
                     /locus_tag="VFQ79_00130"
     CDS             complement(7920..8807)
                     /locus_tag="VFQ79_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0138079.1"
                     /translation="MLQRFLNPSVLAGISGLELVAKTVVDGFVSGLHRSPDFGFSQEF
                     AEYRAYSEGDDLRHIDWNVFARTERAYLKRYRGETNSQLTLLLDASNSMGFGSQPVKK
                     MDYARFLAGSLFYLASQQRDAAGLIVFDDEVRNYIAPSTRQGQLHRLLHGLERAEPNA
                     RTDFAKPFVHFQQFLRRRGIVVVISDFYESPEAVVKTVAPLRFHGNELILFHVLDPQE
                     IRPALDAPALLIDMETGDTIEVSPEYARTEYREKIDSHLERLRDTARSAGMDYFLIDT
                     SRPLDEALREYLTIRQGRM"
     gene            complement(8809..9834)
                     /locus_tag="VFQ79_00135"
     CDS             complement(8809..9834)
                     /locus_tag="VFQ79_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0138080.1"
                     /translation="MSTPTLTFADSAELKSRINRFQEIQNEIVTQVRRVIVGQEEVLE
                     QVMIALFVGGHCLITGLPGTAKTLLVRTIAQTLGLQFKRIQFTPDLMPSDITGTDIIE
                     EEQGTGRRVWTFVPGPIFTNILLADEINRTPPKTQSALLEAMQELACTVRGHNYRLPS
                     PFFVLATQNPIELEGTYPLPEAQLDRFLFNTVLDYLSADQEIEVINLTTTTHVAKADP
                     VTNSEEILEFQQLVRMVPIADSVARYAVELVRATRYSDASAPDFIKKYVNYGASVRAV
                     QFLVLAAKARALMHHRYHVSYEDIRALAVPVLRHRILLNFHAESDRLKPDDLLRQLLD
                     WKPAPRT"
     gene            complement(9843..>10406)
                     /locus_tag="VFQ79_00140"
     CDS             complement(9843..>10406)
                     /locus_tag="VFQ79_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0138081.1"
                     /translation="RKQMKEIAANAKKNDHDEVIRQGRAIRDIYPDYVEAGSVYEFLA
                     DAYLAKNDKTSAIGELETYARTGGRDPALLKKLATLQEEAGKKKEAAETLNRLNYIYP
                     VNDEELHRRLGGLYLETGNLDGAIREYTAVVAMTPQDPAGSHYNLARAYRAANKMTEA
                     REHVFLALEAAPGYKPAQKMLLELSTN"
BASE COUNT         1993 a         3211 c         3090 g         2112 t
ORIGIN      
        1 gaaaccggaa agcagatctg gcacttcgaa gcggacaaag ttgccctgcg tggcctggca
       61 tggtggccgg gcgaccgtaa cacgcatcct cgtgtttttg ccggagtgca gggcgggatg
      121 attgcggtcg acgccaaaac aggcaggccg gcgacgggat tcgcaaacga aggactcctc
      181 gatctgaagc aaggcgtgct tggggatctt cccgatgcgc gcatctcgct ccagtcgccg
      241 ccaaccgtct acaaaaatat tgtcatcacc ggaagcacca atggagaagg ggcgcccaca
      301 aagggtgcct acggtgacat tcggggctgg gacgcttaca cgggcaagct ggtctggagt
      361 ttccacaccg taccgcggcc gggtgaacct ggcaacgaaa cgtggccgcc cggcggctgg
      421 aagaatcgtt ccggcgcgaa tgcctggggg tttctcgcgc tggatgtgaa gcgcggcatc
      481 gtctacgttc cgcttggcgc gcctacctcg gacttctacg gagccgatcg tcacggcgac
      541 gggctgtacg gcaacagcct ggttgcgctg gatgctgcaa cgggcaagct caagtggtat
      601 cagcaacttg tccaccacga cctttgggac ttcgatttgg cagcggcacc gactttgttc
      661 gacgcttccc tcaacggccg cgtcgttcca gccgttgcgc aaatcaccaa aatgggaatc
      721 ctgttcgtct tcgatcgcgt gacaggcaaa ccactgtacg ggatggaaga gcgtccagta
      781 ccgcagagca cagtacctgg agagaagaca gccgccacgc agcccttccc gctcaagccg
      841 cctcccctgt cccgcatcga tttcaccaag gaagagatct acaatttgac gccggagcac
      901 gcagcgttct gccaagacct tttcgagagc aaccaaatgc gaatcggaag cctctacaca
      961 cctcttggcc tggaaggcaa cgtattgatg ttcccgagca cactgggcgg cggcggatgg
     1021 ggcggggtat cgatcgaccc atctcttggg tacttgttta ccaacgtcaa caacctgggg
     1081 cagtggggcc acatggagaa aaggatcgat cggtctaccg gcgccgaaac ctatgtacgg
     1141 acatcggcgc atggaacgta tgcccgcttc tggaaccggg aaacacggat tccctgttct
     1201 aatccgccgt tcggcgaact ggtagcggtg gatacgcgta ccggcgatat cgcgtggcga
     1261 acgccgctgg ggaccgttcc cgcactggaa gaaaaaggtg ttaaaaacac gggcactctc
     1321 aacctcggcg gcagcatcgc taccgctgga gggctcgtgt tcattggcgc aaccaacgac
     1381 agccgctttc gagcctttga atcgaagacg ggacgtttat tgtgggagca gcccatcgac
     1441 gccaatggcc acaccgttcc gatcacgtac ctgggcaagg acggcaaaca atacgtcacc
     1501 atcatggcgg gcgggggcgg cggatatttc ggcggcacgc caagcgatag cgtcgtcgct
     1561 ttcgcgctgg gtgaaggttc cgccccgccg gtcgttacaa agacatcgcc cgctccccag
     1621 agagagtcac ccgtccgggc cgcgggtcca accaccttac cggacggcgc cggaaaagcg
     1681 gccgtcctgc gatcctgcgc cggagcctgt cacgacatgg aagtggtcgt cggcgtccgc
     1741 cgcgaccgcg ccgcctgggc ggccatggta gacaacatga tcgcccgcgg cgcaaaagca
     1801 aaaggcgacg aagtgaagct gatcatcgac tacctgacta cgcatttcgg gaaataagtg
     1861 gtaccggact taaacgctaa gacgttagaa tatctccatg aaattcactg gggatccatc
     1921 actaccgtcc ggtccggcag agttgcaggc gatgctttcg aagcggcttg ccgggccatt
     1981 acctgaatcg tatgaagagc ggctaagctt actcggagac catctgcgcg acgtcacagc
     2041 ttatctggga agtttgactc caaacccctt cgtagctgga ccggaccaga ttgctcaaag
     2101 tatcagcctc gcatctcagt tatcggccga aatccgcagg gcacagcaag cgagcgccct
     2161 tgagcgaatg tccgactgcg atgccgccgc ggcgtggggg gttggattaa taacagtagg
     2221 ggcactgggt ggcggccctg tcggagctgt cgccgggttt gtctatggcg ttgggctcct
     2281 cgccacgagt tgctagtttc gctaagcgag ggcggccact aaattgtgtg gactccctgc
     2341 ctatcgggtc ttgctcaagc gccggaagta ttctgccacc gcgtccgcgt agcccgttgg
     2401 aaccttttcc gaagcgccgg tcagcacttg gtcggattgg ccaccgtcca cctgccggcg
     2461 gagttgcagt tccaattgct cgagtgcggg caggacctgg ccgtggatct tttcaagcag
     2521 cgccgggttg cccggaaatc gcttcggatc gagctgctgc atttcgcgaa tcagttgctg
     2581 gacatcgcga tccatctccg gcgcacccgc ttgcaaatcg cggcgtaact cggaaagatc
     2641 ccgaagccct tcgctgtagg cgcgttcgag tcctcgtacc gctgaaggat ccgccgcacg
     2701 tccaatcggc cccggcaggt cgccacgatt cattgcggtg tatccatcac cgcgttcgag
     2761 tcctccgggg ttgccctggc gtccctgacc atccgcgccg cgctgtccct gcgcgccttg
     2821 ttggcctggc tgcccttgtt gtcctccctg gccttgctgt ccctgttgac cttgctggcc
     2881 ttgctggtct tgttggcctt gctggccacg cgataactgc gacatctggc gctgcaaacg
     2941 gttccgcagt tcttcgacct gcgccagact gcgcttgagg ccgggattct cctggtcccc
     3001 cgacttcgac tctccgagag cgcgctgcgc ctccttcacc tgatcgctca atctatcgag
     3061 gccctgcgta atggtctgct cccgcatcca ggcatacgcg cccaagccgc gccggatata
     3121 ctccgattga aatttcatgc ggaggccgag ttcgttctgc tgcatgtcgc cgagcgcttc
     3181 gcgcagcttc gaggatgccg tgcgctcggt gcccgcaaga tcgcggacac tatccttcat
     3241 gtcgcgctcc aaccgctgga gctcggaaag catgttccgt ttctcatcag cgagccgttc
     3301 cacatcctgg cggctggtgc caggtcgaag cccctggccc tgctcgcggc catacatttg
     3361 gcgcagctta tcggcgaagt ttctctgctg cgatgccagt tccccggcgc gctgcgataa
     3421 gtcgtcgagc tgccccgatg cctgttgccg gcgcatgccg cccaatgcat cgcgagcttc
     3481 ctgaaggcgc tccgccgcac ggcgcgagtc tgcggaattt tgttgttgag agcctgcgtt
     3541 acgcatgtcg cgcgtggcct gctccagccg ttcgagagca cgttccaggc gcgggtcgcc
     3601 acccgattgc ccgcgctgca tgcgtccctg ttgccccgat gaggaagact gaccctgctg
     3661 gccggattgc tgcgattgct gcgacgattg ttgtccggat tgctgcgagc cgctgttttg
     3721 tgccagctcc tgcatgcggc gcttcaactc ttccgcttca cggcgaagca tctcctgctg
     3781 ccagcgttgt tggaacgatt gggactgatt gcgctgctgt tgcgccagct cctgttgtct
     3841 cttcgcgagc tgttccagct tctggagcgc ttcgtcgatt tcgcggctcc gctgttcggt
     3901 tgacgcggag cgctgtcccg tctcgtactg gttcttctcg gtgtcgagtt cgagatcgaa
     3961 aagattctcc agatcacggc ctccctgccc gccgcctccg ccaccgcgcg agccaaacgc
     4021 cacctgtatc tggcggaatg tggattcggc acgcaacaga tgttgaagcg ctttctgctc
     4081 gggtgcaagc gctcctctcc aggcctggga tttcagttga tcgacggcac tgttcattgc
     4141 ctccgccgcg gcttccatat ctttcgagaa gctctggaac tcctggtttg ttccggagag
     4201 ttgccggctg cgcatgcgtt ttgccagcga cctggcctga tcccgcagct tgccctgcac
     4261 ctcggaaagg aactttgcgt tctcggcggc gtgcgtgttt gatttcttct ccgccctgag
     4321 ctcgttccac gtggcggcaa tgatctcctt ctgacgttgc gagatgttgt tgtcctgctg
     4381 ctcgccctcg ccttcgccgc cacccatctg ctgcgactgc gaatactccc gctcgaacgg
     4441 ctgcgcttca atgaaaaaga tatcggtctg gctgttggaa cgcgcgtcgc gcgccacggc
     4501 gtagagactg acaaggtcgc ccggaacgag tttgaaatcc tcgagagcga tcagcgtgga
     4561 cccgtccgct tcttttgcac ctttgttctt gagcagcgga accaccttct cgggaccgcc
     4621 gttcacggca tagcgcaact ccatggcatg cagcccgaaa tcgtccgtcg ctgtaacggc
     4681 taccggcacc tcttcaatcg ggctgaccct ggcatcgcgt cccgggcggc tgatgcggac
     4741 aaggggtggg gtttcgtttt cgatcgaaat aaagaagtcc tcgctcaggc gcaccggcgc
     4801 gtcatgctcg atggcggcca catgatagac ggcatccttc gacatcttca ggcgagccga
     4861 ataccaattc tggcctgttc catttaacgc gagacgattc tcgttatcca caatcagcgc
     4921 accctgcgca agaggccggt cgaactggat agcgacttcc gcctccgttc cctccacggc
     4981 gcgcagatca cccgtcgact catcgaccac gctcttcagg ttcagccacg ccgggtagtt
     5041 gtacgtgatg cgcatactct gaatagcggg aaggtccacc acacccagcg tgaagtgttt
     5101 ggagcggatg ccgccggcct cgacgtaata ttcgatgttc tcggagattc cggccagcaa
     5161 aaactcatag ccggagccgc ccgcttccac ctgcatggga gtctcttccc atttcgtcga
     5221 gctatgaaac cgcgcgaaga gccggacgcg cggcgactgg aatcccatca gattcgcgga
     5281 aacaagctgg tctgaaccgc gccgtatttt ggcgtctccg ggcgaaaccc gaatatcgta
     5341 gaagggctgg ctgccggcct tgggtgtacc accccacaga agcgccgtac catggccgag
     5401 aaatccggga ccggagacgg caagccagac cagcgcgatg accgcggcgg cagcggtgga
     5461 cagaaagccc accaaccagg tgcgcggcgc tacgcgctcc ggttcggccg tgcgggccac
     5521 ttcaacggta ttggcggcga gcagctccag aaacggatcg ggctcctcgc gattcttctc
     5581 ggcgagagtg agcaggcgct gctcgaattc ggggaattgc tgttccgcgc gccgggccgc
     5641 cgcgcgccgg ttcagtttta acagcggcag taccaggccg aatgcgatag cgaaggcaag
     5701 cgcgagaaaa agcagaacgc gcgcaccggt tacgctggtt tccgagaacg caaaacggtt
     5761 agcgagcagt acgagcagca ctgtcgcggc cagagcggca atcgcggcaa tggccgcgcc
     5821 gcgcgagaca gccgcgaact tcaacctcga ctccagtctt cgaaggtatt gatttaactg
     5881 atccagtgcg ttcatgcgat tcccccctgc actttttcag gcgcctctcc ggcgagataa
     5941 cgcgacccaa acatcgattc cgcgatggct gtcaacagga cagcgagcat gacaaaccac
     6001 cacaggtccc agcgcttctc ttcactcgcg ccggtagcgg cagcagcagc ctcggccgga
     6061 cggccggtgt tttcccagag agcaaggctc tcgccggtga ttatatccag gtcggactcg
     6121 ttgcggtcgg cgttcacggc caccatttcg cgctgtccgt tcgctcgatt gatctgataa
     6181 aaacccgctt cggacaactg caagctgttc actttggccg cttcggccag cgtgagggcg
     6241 cgctgcccac gcgggtcgag gacctcgaag gccacgccgt catcccgatt gccgcgcaac
     6301 tccaggtgag cgtccaccag gtagccggcg cttcggcccg catgacccgc aagataacgg
     6361 gcggtctgct cgatgaacgg tacaaatgcg gcatgtagag gaaagtcgtt cgaaatgttg
     6421 tcgaaggtcg aagcaaacgt caggacgcgc ccggtaccta ttgtcttttc caaaagaagg
     6481 ggccgctggt ccgccagccg gatcagcacg cgcgaatctc cgggcgcgat ctccaccgtc
     6541 tgataaaact tgacgccatc cagttgcccc gcgctttgaa gcgagggatg gctcgtgtca
     6601 atggatgcga cggattggaa tcgctcggcg gcgcgggagc tgtatcgtga ggcgatgacc
     6661 tggtctcccg tcaccggaat ccgtgcacgc gtaccgagcg ccggtccggc tgcgatcaac
     6721 accgaaccac cctgctgcaa atacgccttc agtgcctctt cggacgcgct ggaaacagag
     6781 gctgcgtccg aaagcacaac aaaccggtat ttcgagaaat cggcgttgtt tatttgtgcc
     6841 gccgtgaggg cgtccaggat gaaagcggaa ttggacgccg agtcgagcgc ttcacggaag
     6901 tagagaaggc ctcgcggctg gcgagcttcg tggacgaaca acgcgcggct gggttcggcg
     6961 cgttcaatgg cgaagaagaa cgtgtcgtca gcggccagcg tatcggcgct gtcgatgcgc
     7021 acttctccac gattgaatcc atgcggcgct tccaatccat ggaactcgac agtcgctctt
     7081 ccttccgctg gaacgctggc ttctttccgc tctacttctt taccgttcag gaccagcgac
     7141 acggaccgtc gcgctgcggc ggcgccgtat ccggcaatgg ttgcctgaat gcgaacctgc
     7201 ctggtgttat acacgcgggc cggcgcggtg acattctcca ccgcgaagtt cgggacccgc
     7261 gcgggtgcca ggctgtgcag cgacagcttt gtgccatccg ccacttgcag atcggcgaag
     7321 gaaggcggca gcgaactctt ctgtatatcc gtaaaaacgt gcgcttccac aggagcccgg
     7381 gacgatgcgc cgatggagcg caacgcgcgc gcaagctccg cgtaggaact ccgggtatcg
     7441 cctggctcta tctcatcgat ggccgaaacg agctgtccct tgtcattggt gggctgcgtc
     7501 atcagccgca caccggagcc aaatgccagc acctgcgctt catcgccgga ccgcagttcc
     7561 gccagcgccg ccttggcttc ctgcttggcc ctggccaaac ggttcgattc gcgcatgctg
     7621 aaggagttgt ctatggcaac taccagcagc ttgcgcccgc ctccaccggc cggtacggct
     7681 cgcatgatgt agggatgcgc gaacgccaga atcagcagca acagcagtgc gacccgcatc
     7741 gcaaacagcg tgaggtattt caaacggcga tgtttcaccg agctttgtgt ccgccgttcg
     7801 aaaaacatca gcgaactgaa gcgaaagggc gtggtcttgt gctgtttgag taagtgcagc
     7861 cacacgggca atccgatggc ggcaacaccg gcgagaaacc agggagacag aaatcccatc
     7921 tacatcctcc cctgccgaat cgtcagatac tctcgaagcg cttcgtcgag aggacggctg
     7981 gtgtcgatga gaaaataatc catgccggca ctccgggcgg tatcccgaag tctctccagg
     8041 tgcgagtcga tcttttcgcg atactcggtg cgcgcgtact cgggcgagac ctcaatggtg
     8101 tcgcccgtct ccatgtcgat gagcagagcc ggcgcatcca gcgcagggcg gatctcctgc
     8161 ggatccagca cgtgaaacag aatcagctcg ttgccgtgaa atcgcagcgg agcgacggtc
     8221 ttcacgacag cttccggcga ctcatagaaa tccgaaatga cgacgacgat accgcggcgc
     8281 cgcaggaact gctggaagtg tacgaaaggc ttggcaaagt ccgtgcgggc gttcggctct
     8341 gcgcgctcca gtccgtgcaa caggcgatga agctggccct gacgcgtgga aggagcgatg
     8401 taattccgga cctcgtcgtc gaacacaatc aatcccgccg cgtcccgctg ctggctcgcc
     8461 agatagaata gcgacccggc cagaaatcgc gcgtagtcca tcttcttcac gggctgggaa
     8521 ccgaagccca tcgagttgct ggcatccagc agcagcgtca actgcgaatt ggtttcgccg
     8581 cggtagcgct tcagataagc gcgctcggtg cgggcgaata cgttccagtc gatgtgacgc
     8641 agatcatcgc cctctgaata ggcgcggtac tcggcaaact cctggctgaa cccgaaatcc
     8701 ggcgagcggt gtaatcccga aacgaagccg tcaaccacgg ttttggccac aagctccagc
     8761 ccggaaatgc cagccagcac cgaagggttc aggaaccgct gcaacatgtc aggtccttgg
     8821 cgccggcttc caatccagca actggcgcag caggtcgtcg ggtttcaagc gatccgactc
     8881 agcgtgaaag ttcagcagga ttcgatgccg cagcaccgga accgccagcg cgcgaatgtc
     8941 ttcatacgag acgtgatacc ggtgatgcat cagcgcgcga gctttggctg cgagaaccag
     9001 aaactgaacg gcccggacgc tggccccgta gttgacgtac ttcttgataa agtccggggc
     9061 gctggcatcc gagtagcggg tagcgcgcac gagttccacg gcataacgcg caacggaatc
     9121 cgcaatgggg accattcgca ccagttgctg gaactccaga atctcttcgg aattggttac
     9181 cgggtcggct ttggcaacgt gcgtcgtagt cgtgagattg atcacctcaa tctcctggtc
     9241 ggcggacaga taatcgagca ccgtgttaaa gaggaaacgg tccagctgcg cttccggaag
     9301 cggataagtg ccttccagct cgatagggtt ctgtgtcgcc agcacgaaga acgggctcgg
     9361 cagccggtag ttgtggcccc ggacggtgca tgccagctcc tgcatcgctt cgagcagcgc
     9421 ggactgtgtc ttcggcggag tgcggttaat ctcgtcggcg agcaggatgt tggtgaagat
     9481 ggggcccggc acaaaggtcc agacacggcg tcccgttccc tgctcctcct cgataatgtc
     9541 cgtcccggtg atgtcggaag gcatcaggtc cggggtaaac tgaatgcgct tgaactgcaa
     9601 cccgagcgtc tgcgcgatgg tgcgaaccag aagtgttttc gcggtgcccg gcaacccggt
     9661 gatgagacaa tgcccgccga cgaacagcgc gatcatcacc tgctcgagaa cctcttcctg
     9721 gccgacaatt acacggcgca cctgtgtgac gatttcgttt tggatttcct gaaaccggtt
     9781 gatgcggctt ttcagctccg ccgaatccgc aaatgtcaat gttggagtgg acatgattcc
     9841 gttcagttcg tactcaattc caaaagcatc ttctgtgccg gcttgtagcc cggcgccgct
     9901 tcgagtgcca ggaacacgtg ttccctggcc tccgtcattt tgttagcggc gcgatacgcg
     9961 cgcgccaggt tataatgcga gcccgccgga tcctgcggcg tcatcgccac tacggccgta
    10021 tactcgcgaa tcgcgccatc caggttgcca gtttccagat acagcccgcc gagccgccgg
    10081 tgcagttcct catcgttgac cggatagatg taattcagcc ggttcagcgt ttccgcggct
    10141 tctttcttct tgccggcttc ctcctggagt gtggccagct tcttcaacag ggcagggtcg
    10201 cgacctccgg tgcgtgcata ggtttcgagt tccccgatgg cgctggtttt gtcgttcttc
    10261 gccaggtaag catcggctaa aaattcgtag acgctacccg cctccacgta gtccggataa
    10321 atatcgcgaa tggcgcgccc ctggcgaatc acttcgtcgt gatcgttctt tttcgcattc
    10381 gccgcgatct ctttcatctg cttgcg
//
LOCUS       DASTMK010000005         5285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_384421, whole genome shotgun sequence.
ACCESSION   DASTMK010000005 DASTMK010000000
VERSION     DASTMK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384421"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFQ79_00145"
     CDS             <1..294
                     /locus_tag="VFQ79_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138082.1"
                     /translation="VQYVAQEGLRTSDAVSTKRTSGEVLGSMAMFGAIYSLLFWIWLF
                     VLNDKIQKGPKPVHAPERTTARGLREAAAGRTLHEESLSEAKEPLNGEEEQGR"
     gene            298..1317
                     /gene="cydB"
                     /locus_tag="VFQ79_00150"
     CDS             298..1317
                     /gene="cydB"
                     /locus_tag="VFQ79_00150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU0138083.1"
                     /translation="MDLNATWFLLLGVLLAGYAVLDGFDFGVGILHPLARTEREKRLF
                     VNSIGPIWDGNEVWLITFGGALFAAFPMAYASVFSGFYIAFMALLFALIFRAVSIEFR
                     SKRPAAAWQRTWDAGFFLSSLIASLLFGVAVGNSMIGIPLDEMGEFTGGFFDLLGPYP
                     LVAGLVATAMFAMHGSLYLYLKLPSGEARERVRGWMWHTWGIFLVLYILGTMYTLVAI
                     PRATANFEHFPWAAGIVVLNVLAIANIPRSIHAGKPGQAFLSSSTAIICLVVLFGVAL
                     WPNLVTASNNPAHSLTIYNAASSAGTLWTMFLIACIGMPFVLSYTAAVYWTFRGEVEL
                     GEHSY"
     gene            complement(1321..2193)
                     /locus_tag="VFQ79_00155"
     CDS             complement(1321..2193)
                     /locus_tag="VFQ79_00155"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0138084.1"
                     /translation="MHTLYWAGALLLAGTVLRASGAPRDMLVPTGWLAGELNKPDMIV
                     FHVGSQKDYDEGHIPGARLLTLADISITGPGGLRLQLPDVKALEDVLGGAGVSDRSRI
                     VIYAGTDSVQSATRAWFTFDYLGLADRTSLLDGGLSLWRAEGRPVTTEKPVVKPARFT
                     SKPRPELVVDADWVHSHSGQPGVRLLDARTPEFYSGDNAGNMPRAGHVPGARNIPYTT
                     ALNEDRTLKPPETLRELMNAQTGETIVTYCHIGQQATVLYFVARYLGLEPRLYDGSFQ
                     DWSSRPELPVERKP"
     gene            complement(2233..2592)
                     /locus_tag="VFQ79_00160"
     CDS             complement(2233..2592)
                     /locus_tag="VFQ79_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138085.1"
                     /translation="MKRTLLLTSMLSAAVTFFGLWALGMAELPSTVKLENARVKVTGI
                     VYQPGVPRERHIRPTDQVIVFLDDCRYQRIDSATGEKTVRERKSGDVIWHSKGEDAPV
                     LVNLDPKPYRTLLIELK"
     gene            2679..3314
                     /locus_tag="VFQ79_00165"
     CDS             2679..3314
                     /locus_tag="VFQ79_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEU0138086.1"
                     /translation="MADSAKLYDAIVNGNAKDAVAITKEALAENADPLELVTKYMIPA
                     MDEVGRRFECEDYFVPELLLAARAMKGAMELIRPLLVERGTEPSGRVVIGTVKGDLHD
                     IGKNLVASMLEGGGFEVVDLGADVAPEKFVEAVKQGKANVVCLSALLTVTMPSMKTTI
                     AALNEAGLRGQVKILVGGAPVTQQYADEIGADGYAENAGSAVKLARTVVKV"
     gene            3325..4185
                     /locus_tag="VFQ79_00170"
     CDS             3325..4185
                     /locus_tag="VFQ79_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEU0138087.1"
                     /translation="MFADLVKDGPVITDGAWGTELQALGLEYGQCPDAWNLTSPAQVE
                     RVARSYVEAGSRIILTNTFRSNRIALDGYGLSGQCVPLNREGAALSCRAAGEGVLVFG
                     SIGPSGKMLMSGETTEDELHAAFAEQAEALASGGVHGLVVETMSDVGEAILAVKAARG
                     TGLPVIACMVFDSGRNKDRTMMGATPEQCGPALLEAGADFVGANCGVGIEAYVPVCAR
                     LAAACGRPIWIKANAGLPELQNGVAVYRGGPDHFSSHLTALVDAGAKFIGGCCGTTPA
                     FITALRSRLG"
     gene            4186..4941
                     /locus_tag="VFQ79_00175"
     CDS             4186..4941
                     /locus_tag="VFQ79_00175"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEU0138088.1"
                     /translation="MRYKLLSCEILYREICAVIARSKHIIDVEFLPKGLHDLGGARMK
                     DRLQQAVDAVDASRYDAVLLGYALCGNGIAGLQARSIPLVVARAHDCITLFLGSKERY
                     IQYFNGNSGVYFRTTGWIERAQNLEQVPVHMIQGLGASYQDLVAKYGEENARYLHEEM
                     GNFRRHYRRLTYIETGVEPDGSFEERAREEAANQSWEFEKVTGDLTLMQSLVNGEWPE
                     SDFLVVPPGWRIAARYDDSILAAEPPAEGTQTP"
     gene            4938..>5285
                     /locus_tag="VFQ79_00180"
     CDS             4938..>5285
                     /locus_tag="VFQ79_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0138089.1"
                     /translation="MNGRERILSLLNGEQPDRLPFMPITMMFAADQLGVKYRDYVSDF
                     RILGEAQLLTAERFDFDYVSVISDPAREAADLGAAIEWFDDQPPALQEDRALFADKAA
                     LARTRVPDPLGGGR"
BASE COUNT         1018 a         1503 c         1701 g         1063 t
ORIGIN      
        1 gtgcagtatg ttgcgcagga gggtcttcgc accagcgacg ccgtctccac aaagcggacc
       61 agcggcgagg ttctgggctc catggcgatg ttcggagcca tctatagcct cctgttctgg
      121 atatggttgt tcgtcctgaa cgacaagatt cagaaaggtc caaagccggt gcatgctccg
      181 gagcggacga ccgcgcgggg attgcgggaa gcggcggccg gccgcacctt gcacgaagaa
      241 tcgctatccg aagccaagga accactcaac ggtgaagagg agcaagggag ataagctatg
      301 gacctgaacg caacctggtt tcttctgctc ggcgtccttc tcgccggtta tgccgtgttg
      361 gacggcttcg atttcggtgt cggcatcctg catccgctcg ccaggacgga acgcgagaag
      421 cggctgttcg tcaattccat cgggccgatc tgggacggga acgaagtctg gctgattacg
      481 ttcggcgggg cgctgttcgc cgcgttcccg atggcatacg cgtcagtctt ttccgggttt
      541 tatatcgcct tcatggccct gctgttcgca ctgatctttc gcgcggtatc cattgaattc
      601 cggagcaagc ggccggccgc ggcctggcag cggacatggg acgcgggttt cttcctctcc
      661 agcctgatag ccagcctgct gttcggtgtg gcggtaggca acagcatgat aggcattcca
      721 ctggacgaga tgggcgagtt caccggcggc ttcttcgacc tgcttggacc gtatcccctg
      781 gtggccggac tggttgccac cgccatgttc gcgatgcatg gatcgctata cctgtatctg
      841 aagcttcctt ccggggaagc gcgcgaacgc gtacgcggct ggatgtggca cacgtggggc
      901 atctttctgg ttttgtacat cctgggcacc atgtacacgc tggttgcgat tccacgggca
      961 accgcgaatt tcgagcattt cccctgggcc gcgggcattg ttgtgttgaa cgtactggcg
     1021 attgccaaca ttccccgcag catccatgcc ggaaagcccg ggcaggcgtt cctgtcgtcg
     1081 tcaacggcca ttatctgcct ggtggtgttg ttcggcgtgg cactgtggcc gaatctcgtc
     1141 acggcgagca acaacccggc ccacagcctg accatctata atgcggcatc gagcgccgga
     1201 acgctgtgga ccatgttcct cattgcctgc atcggcatgc cgttcgtgtt gtcctatacg
     1261 gcggccgtct attggacatt ccgcggcgag gtggaactgg gcgagcatag ctactgaagg
     1321 tcaaggtttc cgttccaccg gaagttccgg gcggctgctc caatcctgga aggagccgtc
     1381 gtacaggcgc ggttcgagcc cgagatatcg cgccacgaag tagagcacgg tcgcctgctg
     1441 gccgatgtgg cagtacgtca caatggtttc gcctgtttgg gcgttcatca actcgcgcaa
     1501 cgtctccggc ggcttgagcg tccggtcctc gttgagcgct gtcgtgtagg ggatgttccg
     1561 ggctccggga acatgtccgg cgcggggcat gtttccggcg ttatcgcccg agtagaactc
     1621 gggtgttctg gcatccagaa ggcgcacacc gggctgcccg gaatgcgagt gaacccaatc
     1681 ggcgtccaca accagttcag gccggggctt cgacgtgaaa cgcgccggct tcacaaccgg
     1741 cttttccgtt gtaacgggcc gtccttccgc gcgccataaa ctgagcccgc catcgagaag
     1801 agacgtccgg tccgccaggc ccagataatc gaacgtgaac caggcgcgcg tggccgattg
     1861 gaccgaatcc gtacccgcat agatgacaat tcgcgaacga tcggagacgc ccgccccacc
     1921 gagcacatcc tccagcgctt tcacgtccgg caattggagc cgcaggccgc cgggtccggt
     1981 gatcgagata tcggcaagcg tcagaagccg ggctccagga atgtgcccct cgtcatagtc
     2041 tttctgcgag ccgacgtgaa agacgatcat atccggttta ttgagttctc cggcaagcca
     2101 cccggtcggg accagcatat cccgcggtgc gccggacgcc cgcagaacag tcccggccaa
     2161 aagcagcgcc cctgcccagt agagtgtgtg catgtcaccg ttgtaatgga agccgggggc
     2221 tggcgacaag ccctacttca actcgatcag cagcgtgcgg tagggcttcg ggtccaggtt
     2281 aaccagcacc ggggcatcct cgcccttgga atgccagatc acatcgcccg acttacgctc
     2341 gcggaccgtc ttttcgccgg tggcggaatc gatgcgctgg tagcggcaat cgtcgagaaa
     2401 gacgatgacc tggtcggtgg gccgaatatg ccgctcacgt ggaaccccgg gctgatagac
     2461 aattccggtg actttgaccc tcgcattttc gagctttacg gtggacggca gctcagccat
     2521 tcccagtgcc catagaccaa agaacgtcac cgccgcggaa agcatgctgg tgagaagcag
     2581 agttcgtttc atggttattc cttgcgcgtg aggtccatct tattagattc cctgtgcggc
     2641 agcacaaaat agtataggat ctgaaatgga gagtagaaat ggccgattcc gctaagttgt
     2701 atgacgcgat cgtcaatgga aatgcgaaag acgcggtggc gatcacgaag gaggcgcttg
     2761 ccgaaaacgc agacccgctg gaactggtga cgaagtatat gatcccagcc atggacgagg
     2821 tgggccggcg attcgaatgc gaagattatt tcgttcccga gctgttgctc gccgctcgtg
     2881 cgatgaaggg ggcgatggaa ttgatccggc ccttgctggt ggagcgtggc acggaaccgt
     2941 ccggccgggt ggtgatcgga acggtaaagg gcgacctcca tgacatcggc aagaacctgg
     3001 tggcatcgat gctcgaaggc ggcggattcg aagtagtgga tctcggcgcc gatgtcgcgc
     3061 cggagaaatt cgtcgaagcc gtgaagcagg gaaaggcgaa tgtcgtgtgc ctctcggccc
     3121 tgctgaccgt cacgatgccc tctatgaaaa cgacgatcgc cgcactcaat gaggccggtc
     3181 tgcgcggaca agtgaagata ctggtaggcg gcgcgcctgt cacgcagcag tacgccgatg
     3241 agatcggcgc ggatggctac gcggagaatg caggatccgc ggttaagctg gcgcgcacgg
     3301 tcgtaaaggt gtagcttgag cctggtgttc gcagatttgg tcaaagacgg tccggtgatc
     3361 acggacggcg cctggggaac cgagcttcag gcgctgggcc ttgaatacgg acagtgtccg
     3421 gacgcctgga atctcacctc tccggcgcaa gtggagagag tggcgcggtc ctatgtggag
     3481 gcgggcagcc ggattattct caccaacacc ttccgctcga accggattgc gctggacgga
     3541 tacgggctct ccgggcagtg tgttccactg aaccgggagg gtgcggcgct gtcgtgccgg
     3601 gcggctggag agggtgtact ggtcttcggc tccatcggtc ccagcgggaa aatgctgatg
     3661 tccggtgaaa cgacggagga tgaactgcat gccgcgtttg ccgaacaggc ggaagcgctg
     3721 gcctccggcg gcgttcatgg ccttgtggtg gaaacgatgt ccgacgttgg ggaagcgatc
     3781 ctggcggtga aggccgcgcg tggaaccgga ctccccgtaa tcgcctgcat ggtgttcgat
     3841 tccggcagga ataaggatcg caccatgatg ggcgctacgc ccgagcagtg cgggccggcg
     3901 ctcctggaag ccggggccga ttttgtgggc gccaactgcg gcgttggcat tgaggcctac
     3961 gttcctgtct gtgcgcgtct tgctgccgcc tgcgggcgcc cgatctggat caaagcgaac
     4021 gcgggattgc cggagcttca aaacggcgtc gccgtctatc gcggcgggcc ggaccatttt
     4081 tcttctcact tgaccgcgct ggtggatgcg ggagcgaagt tcattggcgg ctgctgcgga
     4141 acaactccgg cgttcatcac cgctttgaga agcaggcttg ggtaagtgcg ctacaaactg
     4201 ctcagttgcg agatcctgta tcgcgaaatc tgcgctgtga ttgcacgatc aaagcacatc
     4261 atcgacgtgg agtttctgcc caagggcttg catgacctgg gtggggcccg gatgaaagat
     4321 cgccttcagc aggccgtgga tgccgtcgat gccagccgtt acgacgccgt gctgctgggc
     4381 tacgcgctct gcgggaacgg catcgcggga ttgcaggccc gctcaatccc tttggtagtt
     4441 gccagggcgc acgactgcat caccctgttt ctcggcagca aagagcggta tatccaatat
     4501 ttcaacggca actccggcgt gtacttccga accacaggct ggatcgagcg cgcacagaac
     4561 ctggaacagg ttcccgtgca tatgattcaa ggcctgggcg cttcctacca ggatctggta
     4621 gcaaagtacg gtgaggaaaa cgcacgctat ttgcacgaag agatgggtaa tttccggcgc
     4681 cattaccgcc gtctgacata catcgagacc ggagtagagc cggacggcag ctttgaggag
     4741 cgcgcgcggg aagaagctgc caaccagagt tgggaatttg aaaaggttac cggggacctg
     4801 acgttgatgc agtctctggt gaacggagaa tggccggagt cggattttct ggtcgtgccg
     4861 ccgggctggc ggatcgcggc acgatacgac gattcgattc tcgccgccga accgcccgcc
     4921 gaaggaacac aaacaccatg aacggccgcg aaagaatact gtctttactc aacggtgaac
     4981 agccggaccg cctgcctttc atgccgatca cgatgatgtt tgcggcggac caactcggcg
     5041 tgaagtatcg cgattatgta tccgatttcc ggatactcgg agaagcgcag cttctgacgg
     5101 cggagcgctt cgatttcgac tacgtttccg tgatttccga tcccgcccgc gaggcggccg
     5161 acctgggcgc ggccatcgag tggttcgacg accagccgcc ggcgttacag gaagatcgcg
     5221 cactatttgc ggataaggcg gcgcttgcgc gaacgagggt tccggatccg ctcggcggcg
     5281 ggcgc
//
LOCUS       DASTMK010000006         5564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_224403, whole genome shotgun sequence.
ACCESSION   DASTMK010000006 DASTMK010000000
VERSION     DASTMK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224403"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1555)
                     /locus_tag="VFQ79_00185"
     CDS             complement(134..1555)
                     /locus_tag="VFQ79_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0138090.1"
                     /translation="MPDEATEESKAQELAAAVREIQERVRSHYATATGSETPVPQPDL
                     MAVLHARDAAEAKVASIGIVNPRPPGFFNSLIQGVKKTVARALDWHVREQVEFNRASV
                     ECVNATLDALNDLNRTMMRLNRGLKRAEEEARELADIRVHWVQWRQEWERKLASTEMQ
                     FLRSVAELEVSFQQRTAQMESAFRELAASQHRDFSVRLDKVASELNAGLLREFDRLRT
                     EHLVRMHADYERTIHSELRLIRQRMATGRSEETASPPVPGNAAPPIDWLKFADRFRGT
                     EEYVKDTQRFYLPHFSRCHYVLDIGCGRGEFLELMQESGVAARGVDLNEECVAICRQK
                     GLEAQLADVFTYLGELPAGGFDGIFCGQVVEHIAPERLPDLIRLMARALQPGGVLVVE
                     TPNPESLAIFATHFYIDPTHVRPVPAALLVFYMEEYGLGGIRVERRFPAVESLPALAQ
                     LPEEVRDSFFGGLDYAAIATKLT"
     gene            complement(1545..1949)
                     /gene="queF"
                     /locus_tag="VFQ79_00190"
     CDS             complement(1545..1949)
                     /gene="queF"
                     /locus_tag="VFQ79_00190"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEU0138091.1"
                     /translation="MKKRQPEYTDEHARAGVAASLPEIETWPNQYSGYEIEIKIPEFT
                     SVCPRTGLPDHGTITITYIPDKVCLELKSLKMYMLAYRNLGIFQENVVNGFVRDIVKA
                     ARPISVTVVGEFTPRGGVYSKITASWSRKRAR"
     gene            complement(1946..3181)
                     /locus_tag="VFQ79_00195"
     CDS             complement(1946..3181)
                     /locus_tag="VFQ79_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEU0138092.1"
                     /translation="MNPSTTVLSLQEVVEAEGHLIDSHIMERIFDTIVEFGGRFEVER
                     FHIGRTNSDPSHLRLRVEATDQRHMEKMLAQLLGLGCSLVDSGDAELKTVDRDFCAPE
                     DFYSTSNHRTLVRHGQQWVEVENQRMDAIIVIENERASCRKLRDIRAGDRVVVGMRGI
                     RVVPESKERDRLAFAFMTNGISSERQVETAVRQAANLITQAHAEGKKVIVVAGPVVVH
                     TGGVQPLSRLIREGYVDAVLSGNALGVHDIEAALFGTSLGIRQSDGRQEEHGHRNHMR
                     AINAIYHAGGIAEAVRSGRLSSGIMYECVKAGIPFVLAGSLRDDGPLPDTITDMNQAQ
                     DAYAEQLKGAGVVLCLGSMLHSIAVGNMSPSWVKLVCVDINPAVATKVSDRGTGQAVG
                     VVTDVGLFLDLLARTLAKA"
     gene            3299..4102
                     /locus_tag="VFQ79_00200"
     CDS             3299..4102
                     /locus_tag="VFQ79_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138093.1"
                     /translation="MAQASAKAQEIRAESRPSDKPLRIEFSPGVLEAIRTEAVDGLFC
                     ELRGGVEIGSILLGLVRDDAAYVTEHIAISHGQDGACAFCLSEGGGDAIADILNVAKR
                     TSPGAPTPVGWYRSGSRTGPDLTAQDLEFHRRFFPDGWQIAVVVQPSILQASEFLCFV
                     PTRDTAGPVRRLRMDCGFPSGPAPSGRLEPQTGVSSRPETHAWNEPVEKSRSRPLWIS
                     AAIGILGIMAICAGIMVLTLAPPEDAVDPLGSTVSTPGQQAPAVGVGGP"
     gene            4167..5066
                     /locus_tag="VFQ79_00205"
     CDS             4167..5066
                     /locus_tag="VFQ79_00205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0138094.1"
                     /translation="MKLSFILVSLPAVLMCQTQPPQTKPEPQPTAASDPVVLSVADEK
                     ITKSEYEQLIRSMPERVQAQAEGAGKRKIAEELARLKAMSQEARKRGLDKTPKVRQQL
                     DFQRDNVLASALFEELSSGIRLDEAALRGYYDQHKSEFEKVKARHILVRFTGSRVPVG
                     EGKKDLSEAEALAKAQNLRERIGAGEDFAAVAKAESDDPGSAPTGGDLGAFGRGQMTG
                     PFEQTAFGLPVGTVSEPVKTEFGYHLLQVQQRAALPFEEVQTQIQNRLKPELARKAVE
                     KIQGQTPVTIDETYFGKPASQTP"
     gene            complement(5069..>5564)
                     /locus_tag="VFQ79_00210"
     CDS             complement(5069..>5564)
                     /locus_tag="VFQ79_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0138095.1"
                     /translation="IVEQFGDLQGKRVLDVGCGKGRFARVLKDRNPGAVVVAFDLAEA
                     MLRFVPQGIHRTAGSMTALPFETGCFDAAYATESLEHAVDIELAVSEMCRVVRPSGPL
                     IIIDKNARHWGRLQTPEWEKWFKRRELKRLLRRHCREVSSRYISYWEDVAPDGLFIAW
                     LARK"
BASE COUNT         1102 a         1702 c         1651 g         1109 t
ORIGIN      
        1 tttgcccagc gccaacgcca tggcctttcc cagccccttg ctcgcacccg tgatcagcgc
       61 attgcgcccc gccagttttc cagccgccat caatcggaat ttctcctcaa tctggctatt
      121 gtaccggagc gtctcaagtg agcttggtgg caatagccgc gtaatccagg ccgccgaaga
      181 agctatcgcg aacctcttcg ggcaattggg ccaatgcggg caacgactcc accgcgggga
      241 atcgccgttc tacccggatg ccgcccagcc catattcctc catgtagaaa accagcaatg
      301 cagccggcac cggccgtacg tgggtcggat cgatgtagaa gtgcgtggcg aaaatagcca
      361 ggctttccgg gttcggagtt tccaccacca gcactccgcc aggttgcaga gccctggcca
      421 tcaaccggat gagatccggc agacgctcgg gcgcaatgtg ttccactacc tggccgcaga
      481 agattccatc gaaaccgccg gcgggcaatt ccccgagata agtaaagaca tccgcgagct
      541 gcgcctccag gcccttttgg cggcagatcg caacgcactc ttcattgagg tcgacgccgc
      601 gggcggccac cccgctctct tgcatcaatt cgagaaactc accccgcccg catcctatat
      661 cgagcacgta gtggcaccgg gaaaaatgag gcagatagaa acgctgtgtg tctttgacat
      721 actcctcggt tccacgaaag cggtccgcga atttgagcca gtcgatgggc ggtgccgcat
      781 taccaggaac aggcggggaa gccgtttctt cgctgcggcc cgtcgccatc ctctgccgga
      841 tcaggcgcag ttcgctatgg atggtccgtt cataatcggc gtgcatccgt acgagatgct
      901 cagttcgcag ccggtcgaat tcgcggagca atccggcgtt gagttccgat gcgactttgt
      961 ccagccggac gctgaagtcc cggtgctgcg atgcggccag ttcccggaag gccgattcca
     1021 tttgcgcggt acgctgttga aaagatactt ccagttcggc aacgctgcga agaaactgca
     1081 tttcggtcga tgccagtttg cgttcccact cctggcgcca ttgcacccaa tgcacccgga
     1141 tgtccgccag ttcgcgcgct tcctcttccg cgcgcttcaa cccccggttc agacgcatca
     1201 tcgtgcggtt caaatcgttg agcgcatcga gtgttgcatt tacgcattcc accgaggccc
     1261 ggttgaactc cacctgttca cgcacgtgcc agtcgagcgc gcgggcgact gtcttcttga
     1321 cgccttgtat cagggaattg aaaaaccccg ggggtctggg gtttacaatg ccgatggagg
     1381 ccaccttagc ttccgccgcg tcccgcgcgt gcaggaccgc catcaagtcg ggctgtggta
     1441 cgggtgtttc ggaaccggtg gccgtggcgt agtgcgaccg gacgcgttcc tgtatttcgc
     1501 gaaccgcggc ggctagttcc tgtgccttcg actcctcggt cgcttcatcg ggcacgttta
     1561 cggctccagc ttgcggtgat tttgctgtag acgcctccgc gcggcgtaaa ttcccctaca
     1621 accgtgaccg atatgggccg ggccgctttc acgatatcgc gcacgaagcc gttgaccacg
     1681 ttctcctgga agatgccgag gttccggtat gccagcatgt acattttcag cgatttcagc
     1741 tccaggcaga ccttgtccgg aatgtaagtg atcgttatgg tgccatggtc cggaaggccc
     1801 gttcgggggc agaccgaggt gaattccggt atcttgatct cgatctcgta gccggagtac
     1861 tgattcggcc aggtctcgat ttcgggcagc gacgcggcca ctccggcgcg cgcgtgctcg
     1921 tctgtatact ccggttgtct ctttttcatg ccttcgccag agtgcgagcc agaagatcca
     1981 ggaagaggcc gacatcggtt acaacgccta cagcttgtcc ggtgccgcgg tcgctgactt
     2041 tcgtggcaac cgccggattg atgtcaacac acacgagttt gacccaactg ggggacatgt
     2101 tgcccacggc aatggaatgc agcatggaac cgagacagag caccacgccg gcacctttca
     2161 actgctcagc gtaggcgtcc tgcgcctggt tcatgtcggt gatcgtatcc gggagagggc
     2221 cgtcgtcgcg caagctgccg gcgaggacga acgggatacc tgccttcacg cactcataca
     2281 tgatgccgct ggaaagcctg ccgctccgga cagcctcggc gattccaccg gcatgataga
     2341 tggcattgat ggcccgcatg tggttgcgat gtccgtgctc ttcctgccgg ccgtcggact
     2401 ggcgaatgcc gagcgaagtc ccgaagaggg cggcttcgat atcgtgcact cccagcgcgt
     2461 ttccgctaag aacggcgtcg acgtagcctt cccgaatgag ccggctcaag ggctgcaccc
     2521 cgccggtatg aactacaaca gggccggcta cgacaatcac tttctttccc tcggcgtggg
     2581 cctgcgtgat caggttcgcg gcctgccgga cagcggtctc cacctgccgt tccgacgaaa
     2641 tgccattggt cataaacgcg aaggcgagcc ggtcgcgctc tttcgattcg ggcaccaccc
     2701 ggataccgcg cataccgacg acgacccggt cgccggcacg aatatcgcgc aactttctgc
     2761 aggaggcgcg ctcattctcg atgacgatga tggcatccat ccgctggttc tccacctcca
     2821 cccattgctg gccgtggcgc accagcgtgc ggtggttgct ggtggaatag aaatcctcgg
     2881 gagcacagaa atcgcgatcc acggtcttca gttccgcgtc gccggagtct accagggaac
     2941 agccaagtcc cagcagttgc gccagcatct tctccatgtg gcgctgatcc gtcgcttcca
     3001 cgcgcagcct caggtgcgat ggatcgctgt tggtgcgccc gatgtggaag cgctccactt
     3061 cgaaacgtcc gccgaactcg acgatggtgt cgaagatccg ctccatgata tgggaatcga
     3121 tcagatgtcc ttccgcttcg accacttcct gaagcgaaag cacggttgta ctggggttca
     3181 tgatggtgta cctacaggat aaccgcttcg gcgcggctgc cgctgtcgcg gccgctttac
     3241 caccggctca cgaaactctt gtgacacact ttcctccgaa acagcatcgt ataaaaaaat
     3301 ggctcaagcg agtgcaaagg cgcaggagat ccgggccgag tcgcgcccgt ccgataaacc
     3361 gctgagaatc gagttttcac ccggcgtgtt ggaggcgatc cggaccgaag ctgtcgacgg
     3421 actgttttgc gaactgcgtg gaggcgtgga aatcggaagc attctgctgg gattggtccg
     3481 ggacgatgcc gcgtatgtca cggaacacat tgctatttcg cacggccagg acggtgcgtg
     3541 cgcattctgt ctatccgaag ggggcggcga cgcgattgcg gacattctca acgtcgcgaa
     3601 gcgaacctca cccggtgctc ccacaccagt cggttggtat cggtccggtt cacgcaccgg
     3661 accggatctg actgctcagg acctggagtt tcaccggcga ttctttcctg acgggtggca
     3721 aatagccgtc gtggtgcagc cttccattct gcaggcgagc gaatttcttt gctttgttcc
     3781 aactcgcgac accgccggcc cggttcgccg gttacggatg gattgcggat ttccgtccgg
     3841 acccgcccct tccggacgcc tcgaaccgca aacaggggtg tcgtcgcgtc cggagacgca
     3901 tgcatggaac gagcctgtcg aaaaatcacg ttcccggccg ctttggataa gtgcggccat
     3961 cggcatcctt gggatcatgg cgatctgcgc ggggatcatg gtgttgacgc tcgctccgcc
     4021 agaagatgct gtagacccat taggctcgac cgtttccaca ccggggcagc aggcgccggc
     4081 ggtgggagtg ggcggtccgt aaacagggta gagtattgct atcctgtgtt atagcctcga
     4141 aaactccaca gtagtgaaga atccccatga aattgagttt catccttgtt tctttaccgg
     4201 cggtcctaat gtgccagacc cagccgccgc aaacgaaacc ggaaccacaa ccaacagcgg
     4261 cttccgatcc cgtcgtcttg tccgtcgcgg atgagaaaat caccaagtcc gagtacgagc
     4321 aactcatccg ctccatgcca gagcgggtgc aggctcaagc cgagggagca ggaaagcgga
     4381 aaatcgcaga agagctggca cgcctcaagg cgatgtcaca ggaggcgcgg aaacggggtc
     4441 tcgacaaaac gccgaaggtc cgccagcagt tggatttcca gcgcgataac gttcttgcca
     4501 gcgcgctgtt cgaggaactc agctccggca tccgtctcga cgaagcggcg ctgcgcggat
     4561 actacgacca gcacaagtca gaatttgaaa aagtgaaagc gcggcacatc ctggttcgtt
     4621 tcacgggctc gcgggtgcct gtcggagagg gaaagaaaga cttatccgaa gcggaagcgc
     4681 tcgcgaaagc acagaacctg cgggagcgca tcggagcggg ggaggacttc gcagcggtgg
     4741 cgaaagcgga atcggacgat ccgggttcgg ctcccaccgg cggcgacctg ggcgctttcg
     4801 ggcgtggaca gatgacggga cccttcgagc agactgcttt tgggcttccc gtgggcacgg
     4861 tcagcgaacc ggtgaagacg gaattcggct atcacctgtt acaggtacag cagcgcgcgg
     4921 cgctcccgtt cgaagaggtc cagacgcaaa tccagaaccg gttgaagccg gagctggcgc
     4981 gcaaagcggt ggaaaagatc cagggacaaa cgccggtaac cattgacgaa acgtattttg
     5041 gaaaacccgc ttcgcagacg ccgtagattc atttgcgggc cagccacgcg ataaagaggc
     5101 cgtccggcgc cacatcttcc cagtaggaga tgtagcggct ggagacctcg cggcaatggc
     5161 ggcgtagaag ccgtttgagc tcacggcgct taaaccactt ttcccattcc ggcgtctgga
     5221 gccgccccca gtgtctggcg tttttgtcga tgatgatcag cggtccggaa ggccggacca
     5281 cacggcacat ctcggacacc gccagttcga tatcgacggc gtgctcgagc gactccgttg
     5341 cgtaggctgc gtcgaagcag cctgtttcga aaggcagcgc cgtcatcgac cccgccgtgc
     5401 gatggatgcc ctgcggcaca aaccggagca tggcttccgc cagatcgaac gctaccacta
     5461 cggccccggg attccggtcc ttcagcacac gggcaaatct tcccttgccg cagccgacgt
     5521 cgaggacacg tttcccctga agatcgccga actgctcgac aatc
//
LOCUS       DASTMK010000007         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_160735, whole genome shotgun sequence.
ACCESSION   DASTMK010000007 DASTMK010000000
VERSION     DASTMK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160735"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFQ79_00215"
     CDS             <1..801
                     /locus_tag="VFQ79_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU0138096.1"
                     /translation="RRMMKSPLSGRSPLAYKNEAFLESADARPIRILSEYLEPLSHLK
                     REKVRDTIVFFGSARITERGPMARYYEDARTLARMLTEWSDGLENSTCRFVICTGGGP
                     GIMEAGNRGAQEAGGKTIGLNIGLPFEQLPNPYITPELSFEFHYFFMRKFWFAYLAKA
                     LVVFPGGFGTLDELAETLTLVQTQKLQKKIVILLYGASFWKEVLNFDALIKYGTISEA
                     DLNLFTYADDPQTAFNVLRDGLTDLYLKPREPLPEQEQKTPAIAKSRF"
     gene            809..1573
                     /locus_tag="VFQ79_00220"
     CDS             809..1573
                     /locus_tag="VFQ79_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEU0138097.1"
                     /translation="MRKNSIPRWVVLLLLIAGPAQPWGEKGHRVVAMIAGERLSAEAR
                     RGVRDLLGSESLVEVSTWADEIRRYRPETGPWHYINVPMEARNPDILSFCPSDGCVVS
                     KMDDFRSMLTNPREKPANRRDALKFLVHLVGDLHQPLHVGGRNDKGGNRTQLVYFGTS
                     TNLHRVWDTEILRRMEGEASLASGLSRTARAGWSRGTPVEWAAESHAVAREVAYKHLS
                     RKAGIPYQQRAETAVRVQLAKAGVRLAAMLNRIWEQ"
     gene            1807..2409
                     /locus_tag="VFQ79_00225"
     CDS             1807..2409
                     /locus_tag="VFQ79_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU0138098.1"
                     /translation="MDVTGGHGGGRYHESMFTVERLIRNRGGLEVANSTVDQPLEHLV
                     ACHGRIEERLAILERAGAVLDTHTAEALDAVQGCFRYFDTSGIMHTADEEESTFPRLR
                     PRLTPEQIGEIERLERDHRIADEMYERLKAVTTRLRAGVTPESVEEYRGIVRQFTAHY
                     RAHIEFENTRLIAVCRENLAPEELQAISTEMKRRRGLIRD"
     gene            2465..3529
                     /locus_tag="VFQ79_00230"
     CDS             2465..3529
                     /locus_tag="VFQ79_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU0138099.1"
                     /translation="MISGMELQAADTVPVKKKKKLVRRNAPDRSQTLRRTVQFLFLAL
                     NIWIGIEFYLWVRFFETGGTSLRVERPPGVEGWLPIASLMNLKTLLVTGYVPGVHVAG
                     MFLLIAFLAISFLFRKSFCSWLCPVGTISEYLWRLGRDTFKKNFSLPRWLDIPLRSLK
                     YILLGLFLYAVGSMSVEASRAFLEGPYGLISDVKMLNFFRFVGMTSAITLAVLVILSV
                     FVQNFWCRYLCPYGALMGIAAFFSPARIHRNPETCIDCAKCAKACPSLLPVDRLITIQ
                     SVECTGCLECVAVCPAEDALHMSLPGKRVLPAWAMAAAVCAIFLGVVAYAQLAGYWHT
                     PIADAIYFELIPRAHEFGHP"
BASE COUNT          726 a         1070 c         1121 g          759 t
ORIGIN      
        1 cgacgaatga tgaaatcacc tctctccggc cgaagtcctc tggcctataa gaacgaagcg
       61 tttctcgaga gcgcggatgc gcgcccgatc cggattctgt ccgagtacct cgagccttta
      121 agtcatctga agcgggaaaa ggtccgcgat acgatcgtgt tcttcggttc cgcgcgcatc
      181 accgagcggg gaccaatggc gcgctactac gaggacgcgc gaaccctggc gcgcatgttg
      241 acggaatggt ccgacgggtt ggagaactcc acgtgccggt ttgtgatctg caccggcggc
      301 ggtccgggaa ttatggaggc cggcaatcgc ggggcccagg aggccggcgg caaaacgatc
      361 ggcctcaaca tcggcttacc cttcgagcag ttgcccaatc cgtacatcac cccggaactc
      421 tccttcgagt tccactattt tttcatgcgc aaattctggt tcgcctacct ggccaaggct
      481 ctcgtcgtgt ttccgggagg attcggaacg ctggatgaac tggcggaaac gctcaccctg
      541 gtacagacac agaaacttca gaagaaaatc gtaatcctgc tgtatggcgc cagcttctgg
      601 aaagaggttc tgaatttcga tgcgctgata aaatacggca ccatcagtga ggcggacctc
      661 aatttgttca cttacgccga cgatccccaa accgcgttca atgttctgcg cgacggcctg
      721 acggatctct atctgaaacc cagggaaccg ttgcccgagc aggaacagaa aacgccggct
      781 attgcgaaat cgagattctg acgcgcctgt gaggaagaat tcaattccgc ggtgggtggt
      841 cctccttctt ctgattgcgg gaccggcgca gccctgggga gaaaaagggc atcgcgtggt
      901 tgcgatgatt gccggagagc gcttatcggc cgaggcgcgg cgaggcgtcc gggatttgtt
      961 gggaagcgaa tccctcgtgg aagtgtctac gtgggcggat gaaatcaggc gttaccggcc
     1021 ggaaaccggt ccatggcatt acatcaacgt tcccatggag gcccgtaatc cggacatcct
     1081 gagtttctgc ccatcggacg gctgtgtcgt atcgaagatg gacgattttc gatccatgct
     1141 caccaatcct cgcgagaagc cggcaaaccg gcgggacgcg ctgaagttcc tcgtacacct
     1201 ggtcggggac cttcaccagc ctttacacgt gggcggccgg aacgacaagg gcggcaaccg
     1261 gacacagctc gtctactttg gcacttcgac caacttgcac cgcgtgtggg acacggagat
     1321 attgcggcgc atggaagggg aagcttcgct ggcatccggg ctgtcccgga ccgcccgagc
     1381 cggatggtcg cgcggaacgc ccgtcgagtg ggccgcggag tcgcatgccg tggcgcgcga
     1441 agttgcgtat aagcacctct cgcggaaggc gggcataccc taccagcagc gggcggagac
     1501 ggccgtccgt gtgcaacttg ccaaagcagg cgttcgtctc gccgctatgc taaaccggat
     1561 ttgggagcaa tagcccgatt gcgtcttccg gacacacttc ggcgcactgc ggctcgtcgg
     1621 aatagccgac acactccgtg cagaaccagt gattgatctt gtaaaccgga gccccttccg
     1681 tgatggcgta gttcgggcac gcagacaggc agatgccgca tcccgtacag tgttcatgaa
     1741 tttcatacgc catacaaagc cacctctctc ctacgatgtt gcggcattcg gcgtgtcgaa
     1801 tctgtgatgg acgtcacagg cggccatggc ggagggcgat accatgaaag catgtttacg
     1861 gtagaacgtt taatccgtaa ccggggggga ctggaggtcg ccaactctac ggtagaccaa
     1921 cctctggagc acctggttgc ctgtcacggg cgcattgaag aaaggctggc gattctggag
     1981 cgggccggcg ccgtgcttga tacccacacc gcggaggccc tggatgcggt tcaaggctgc
     2041 ttccgctact tcgataccag cggaatcatg catacggccg acgaggagga gagcacgttc
     2101 ccgcggttgc ggccgcggct gactccggag cagatcggcg aaatcgaacg attggaacgg
     2161 gaccaccgga tcgccgacga aatgtacgag aggctgaaag cggtcaccac gcggcttcgg
     2221 gcgggcgtta cgccggagtc tgtcgaggag taccgcggca tcgtgcggca gtttacggcc
     2281 cactaccgcg cgcacatcga attcgagaac acacggctga tcgccgtatg ccgcgagaac
     2341 ctcgcaccgg aagagcttca ggcaatttcg acggaaatga agcggcggcg cggcctgatt
     2401 cgtgactgag gtcactgtcc ggagctgagt gccaacgtac gctgaatgta cagggagcga
     2461 tccgatgatt tccgggatgg aactccaggc cgcggacact gtaccggtga aaaaaaagaa
     2521 gaaactggta cggcggaacg cgcctgaccg ctcgcagacg ctgcgccgca cggtgcaatt
     2581 tctgtttctg gcgctcaaca tctggatcgg catcgagttt tatctctggg tacgcttctt
     2641 tgagaccggt gggacctcgc tccgcgtgga gcggcctccg ggagtggagg gctggctgcc
     2701 catcgcctcc ctgatgaatc tcaaaacctt gctggtgacg ggatatgttc cgggcgtgca
     2761 tgtggcaggc atgttcctgc tcatcgcatt cttagcgatt tcgttcctgt ttcgaaaatc
     2821 cttttgcagt tggctatgtc ccgtcggcac gatatcggag tatctgtgga ggctgggccg
     2881 ggacaccttc aaaaagaact tcagccttcc ccgctggctc gacatccctc tgcgcagcct
     2941 gaaatacatc ctgctcggcc tgttcctcta cgccgtcggc agcatgtccg tcgaggcgag
     3001 ccgcgcgttt ctcgaggggc catacgggct catctccgat gtgaagatgc tgaatttttt
     3061 ccgcttcgtt ggaatgactt cggccatcac gctggcggtt ctggtgattc tctcggtatt
     3121 cgtacagaac ttctggtgcc gttatctctg cccgtacgga gccctgatgg gcatcgcggc
     3181 gtttttcagt cccgcgcgca tccaccgcaa tccggaaacc tgtatcgact gcgccaaatg
     3241 cgccaaggcc tgcccatcgt tgttgccggt ggaccggctc attactatcc agtcggtgga
     3301 atgcaccggc tgtctggaat gcgtagcggt gtgtccggcc gaggacgctc tccatatgag
     3361 tctgcccggc aaacgcgttc tgcccgcgtg ggcgatggcg gctgccgtct gcgccatctt
     3421 cctcggcgtt gtggcgtatg cccagcttgc cggctactgg catacgccga tagcggatgc
     3481 gatctatttt gagctgattc cccgggccca cgaattcggc catccttgaa agatccaggg
     3541 cgtgggcgcc ccggttcccg gcactcgaac gctgtgctaa cctgaagcgt ttggcactgc
     3601 ccgagttatg acgcccactc gcgagaagac tcaactcatg agcgcttccg agatcgatcg
     3661 gacgctggtt cggctg
//
LOCUS       DASTMK010000008         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_320328, whole genome shotgun sequence.
ACCESSION   DASTMK010000008 DASTMK010000000
VERSION     DASTMK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320328"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFQ79_00235"
     CDS             complement(<1..200)
                     /locus_tag="VFQ79_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138100.1"
                     /translation="MKRLKKLWFRLIRKDPEAVVVSFLTGEEPAARAMLEEIRALVPD
                     RHHYAAGFRRPEFLSAEVEFLQL"
     gene            complement(197..1375)
                     /locus_tag="VFQ79_00240"
     CDS             complement(197..1375)
                     /locus_tag="VFQ79_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0138101.1"
                     /translation="MPIRWVVLGVFVLSSALNYLDRQILAQLAPVLKAEFHLSNQDFG
                     FVLSAFSILYAACAPFAGLFVDRAGLNRAASIAVATWSLAGIGTGLVSGFAGLLGARA
                     LLGLAESAGVPASGKAVRLYLKPEERALGGAVSQLGLSVGSFLAPPIATFLALWYGWR
                     SAFILTGLLGFLWIPLWLATAKRFPPRAEAQSGTTRGASGILRDSRLWAFATANILGM
                     AVYTLWSNWITIFLMQTYGLQLVETPWLAGIPPLVAPLGGFFGGWISLRLVRRGMEAP
                     KSRRMACFASAIALLGTAAVPWMPSAGLATAAVAISFFWASSYSVNLYALPLDVYGAE
                     HAAFSTSILTCAYGVLQALFSPVIGGMVDRYGFEPVCLLVAILPLVGTGVLKLAEKRQ
                     "
     gene            complement(1387..>2032)
                     /locus_tag="VFQ79_00245"
     CDS             complement(1387..>2032)
                     /locus_tag="VFQ79_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138102.1"
                     /translation="DTPEPAQKIDHLTKATAIDPRNSKLWKELAELLEAENRFADAAK
                     AWAGAERAAASAEEREKLSQIRRENDQKRIEAAYAEKMRIAAEKQRELDQLRDEALTR
                     IREAEEKANAGATPHEGKVEQWWTGPTASGRITGVLQQVECGKSQTRLIVQANGKTTR
                     LLIRDPSKIVVQGTRQETLSCGPQRPPRPVAIDYFEKPDAKTATAGEAAVIEFR"
BASE COUNT          404 a          660 c          592 g          376 t
ORIGIN      
        1 agttggagga actctacctc ggcggagagg aattccggtc ttcggaagcc cgccgcgtaa
       61 tgatgccggt ccggcaccag cgcccggatc tcctcgagca tggccctcgc ggccggttct
      121 tctcccgtga gaaaactcac caccaccgct tccggatctt tgcgaatcaa ccggaaccac
      181 agcttcttca gtctcttcac tgtcgttttt ccgccagctt cagtacaccg gtacccacga
      241 gcggcagtat cgcaaccagc aggcagacgg gttcaaaacc gtatcgatcg accatgccgc
      301 cgataacggg cgaaaagagc gcctgcagaa ccccgtaggc gcaagtcaga atcgacgtgc
      361 tgaatgcggc gtgttcggct ccgtacacat cgagcggcag cgcatagagg ttcacgctgt
      421 acgatgacgc ccagaaaaag ctgatggcga cggccgccgt ggcgagcccg gcactgggca
      481 tccagggaac agcggcggta ccgagcagcg cgatcgcact ggcgaaacaa gccatccggc
      541 gggacttcgg agcttccatt ccacgcctca ccaggcgtaa ggagatccaa cccccgaaaa
      601 agccgcccag cggcgctacc aggggcggaa tgccggcaag ccagggtgtc tcgaccagtt
      661 gcaggccgta ggtctgcatc aggaaaatcg tgatccagtt actccagagc gtatacaccg
      721 ccatgccgag tatattggcc gtggcgaagg cccacaaccg cgaatcacgc agaataccgg
      781 acgcgccgcg ggttgtcccc gactgtgcct ccgctcgcgg tggaaagcgc ttcgcagtcg
      841 ccagccatag tgggatccag aggaagccca gcaatcccgt caggatgaaa gcagaacgcc
      901 atccgtacca cagagccaga aatgtggcga tcgggggcgc gagaaaggag cccacgctca
      961 acccgagttg gctcaccgct cctccgagcg cacgctcttc cggcttcaag tacaggcgga
     1021 ccgccttgcc ggacgcaggc acaccggcgg attccgccaa ccccagcaag gcgcgcgctc
     1081 ccagcaagcc cgcgaaaccc gagaccaggc ccgttccgat tccggccagc gaccatgtgg
     1141 cgacggcaat acttgccgcg cggttcaaac cagcccggtc gacgaagagg cccgcaaacg
     1201 gtgcgcaggc cgcgtagagg atagagaacg cggacagaac aaacccaaag tcctggttgc
     1261 tgagatggaa ttcggctttc agcaccggag caagctgcgc gaggatctga cggtccagat
     1321 aattcagggc ggacgatagt acgaaaactc caagcactac ccatcggatg ggcattcaga
     1381 agatcatcac cggaactcga tgaccgccgc ctctcccgcg gttgcggtct ttgcgtcggg
     1441 cttctcgaaa tagtcgatag caaccggccg cggaggacgc tgcggcccgc agctcagtgt
     1501 ctcctgacgc gtcccctgca cgacgatttt cgatggatcg cgaatcagca ggcgtgtcgt
     1561 cttcccattc gcctgcacaa tcagacgtgt ctgcgacttg ccgcattcca cctgttgaag
     1621 cacgccggtg attcgtccgg atgcggtggg gccggtccac cattgctcca ccttcccttc
     1681 gtgcggcgtg gcgccggcat tggctttctc ttccgcctcc cgaattcttg tgagcgcttc
     1741 gtcgcgtagt tgatcaagct cgcgctgctt ttccgctgcg attcgcatct tctcggcgta
     1801 tgctgcctcg atgcgcttct ggtcgttctc ccggcgaatt tggctgagct tctcgcgttc
     1861 ttcggccgac gccgcagccc gctccgcgcc cgcccaggct tttgccgcgt ccgcaaaacg
     1921 gttttcggct tcgagcaatt cggcaagttc cttccacagt ttcgaattgc gcggatcaat
     1981 cgccgtggct tttgtcagat ggtcgatttt ttgggctggt tccggtgtgt cg
//
LOCUS       DASTMK010000009        17428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_608834, whole genome shotgun sequence.
ACCESSION   DASTMK010000009 DASTMK010000000
VERSION     DASTMK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608834"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFQ79_00250"
     CDS             <1..728
                     /locus_tag="VFQ79_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138103.1"
                     /translation="IDRLSDAGVATFNLAVDSVEDREELPKALNPIRREFEYLITKQY
                     VYDYSVFFNINITRINLEDVKALTEIAHDVGIATDYHINEAPMLDQPHFKHTDANETF
                     ITPEDWPQVDSVIDWLIEKSRSGFKMVNSIRRLEEMKQFMRGKLQDWNCRAGQNSLII
                     RTDGTLAPCFPMYSASYDWGVASQPSFNIHQLQDMKRECQPHCFSTLNHNLAYCYDAG
                     RAFKWVLKQALRGFRGTTGGFDE"
     gene            725..1165
                     /locus_tag="VFQ79_00255"
     CDS             725..1165
                     /locus_tag="VFQ79_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU0138104.1"
                     /translation="MRLEIDCEPGGDEVEALLTEDQRLHIRNALEARRRELIDGIRSQ
                     AVELAVSDGIHDPIDKVQMMAYRDEAVQQVGRLSETLSSVEKSLAALSGGYYGHCAEC
                     GKPIAAKRLEVIPWASHCLNCQARRESNHHAGAGPSGDEREAAA"
     gene            complement(1190..1828)
                     /locus_tag="VFQ79_00260"
     CDS             complement(1190..1828)
                     /locus_tag="VFQ79_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0138105.1"
                     /translation="MADCGRESKRLASADRRSQIFEIAAAQFARTGLHGTTTQALAKA
                     AGVSEPVLYVHFNSKERLFRDAVENNIETRLRALDDRLASIGRKSPLECIESMAEATV
                     SVCVSGASNAVLTNWALLEAPEYAADLHRNEVGSVGIKWQCELALHFPDSRSRVILSI
                     HLVPYAVNACLAYGFWLATLRHTSESATALAQQFAAGVAQAASALLSGQSKE"
     gene            complement(1821..2102)
                     /locus_tag="VFQ79_00265"
     CDS             complement(1821..2102)
                     /locus_tag="VFQ79_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138106.1"
                     /translation="MFHVGRTEEQSGTVITIDGKLSHDCIEFVETYCSQEISTGKPVA
                     LFLRDLTIIDEAGRALLRRLTAKGVRLLASGVYTSYIVQGLIPTKDADG"
     gene            2225..4327
                     /locus_tag="VFQ79_00270"
     CDS             2225..4327
                     /locus_tag="VFQ79_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0138107.1"
                     /translation="MPTIDICQRQLPKSAPPGSILDMGIPDSKIYKALFDLSQSIAGH
                     TDLETLCNSLADSLRHVVSFDFLALVLHDPVRDQLRLHAISTSRPYKNHREVVLPADG
                     DHFGALVWRDQKPLVLSPLYKETQYRDVVDEALQAGIQAIILVPLSNGDRRLGILGFG
                     FTAPFQPDEGAVAFLQRVASEVAVSVEGYLTRHALLHERDRIRVLFEITNALVSKLPL
                     EELFSAISEQISRVVEHDFAVVGLLDKDTGRIHLSGLHSPGGIQVEIEETSGDPEGLP
                     PGEALATGKPVVITEPDFERFRSPRYRKYFEMGFRSGCAIPFTGVNGISGVLSLARKS
                     GEPFTNDEVDLLVQVARQIAIATENSLAYRELLQIKDKLATEKLYLEDEIRTDQNLGN
                     MIGESPAFQAVLSAIQVVAPTDATVLIEGETGTGKELVARALHDLSGRSQRSFIKVNC
                     AAIPATLLESELFGHEKGSFTGAFAQKIGRFELAHQGTLFLDEVGEIPLELQSKLLRA
                     IQEQELERLGGNRTIRVDVRFIAATNRSLKQMVDEGKFRSDLYYRLHVFPLMVPPLRE
                     RKEDIPLLIRYFTQKYAKRMNRPIESVPSAAIEALTNYEWPGNIRELQNVIERSVILS
                     PGRVLQIAIPAIAEPSSLAARTPRLEESAERERILRALHESHGKVAGPNGAAARLGFR
                     RTTLQSRMKKLGIERQYR"
     gene            4453..5688
                     /locus_tag="VFQ79_00275"
     CDS             4453..5688
                     /locus_tag="VFQ79_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0138108.1"
                     /translation="MRNEHLVVTGLVLSVLVSSSCKPKASAVPSQAGPAEVATVTIRP
                     ERTVLTTELPGRTSAYLVAEIRPQVNGLIQNRLFREGSNVKAGDLLYQIDPAPYQAAY
                     DQAKAALVTAEADLVTAEANLPAIRSRAERLKGLVAIHAVSQQDYDDASAALRQAEAS
                     LAARKASVEINRAAVESARINLSFTPIKAPISGRIGISSITAGALATAYQPTPLAVIQ
                     QLDPIYVDVVQANAELLRLRQRLESGRLKRNGTVQNKVKLFLEDKTPYPLAGTLQFQD
                     VTVDPATGSVTLRLVFPNPKQVLLPGMFVRAVVQEGVSEQALLVPQQGVTRNTKGIPV
                     AWVVGQDEKVEERILELDRAIGDKWLVTSGLVAGDRLIVEGFQKVRPGAQVRAVPFDA
                     NGSPAASGGQDKANGGSGV"
     gene            5681..8830
                     /locus_tag="VFQ79_00280"
     CDS             5681..8830
                     /locus_tag="VFQ79_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0138109.1"
                     /translation="MSKFFLHRPVFAWVIAIGMMVGGALAIYNLPISQYPPIAPPSIS
                     IRAVYPGATAKTVEDSVVQIIEQKMTGLDRMLYISATSDSSGSGVLELTFAPGTDPDL
                     AWSKVQNKLQLAMPMLPEVVQRQGVTVAKSTRNYLIMVGIASTTDNMDLYDLNDYAVS
                     QIQPSLARVPGVGEVEAFGSQYSMRVWMDPNKLTQYNMTVSDIIAAIRTYNVQVSAGQ
                     FGGMPAVQGQRLNASILVQSLLKTPEEFGAIPLRNNPDGSIVRVRDIARTELGTEFYD
                     VQASYNGRPAAVLPVRQEPGANALDTAAAVKAKMEELSQYFPPGVQVIYPYDTTPFVR
                     VAIGEVVRTLFETIFLVFLIMYLFLGNMRATLVPTIAVPVVILGTFGVLALFGFSINM
                     LTMFAMVLAIGLLVDDAIVVVENVERVMSEEGLSPWEATMKSMDEITGALVGIGCVLS
                     AVFAPMIFFPGSTGVIYRQFSVTVIASMLLSVVVALVLTPVLCASLLKPVPKGHEAAE
                     TGFRLLRPFFLAFDRAFNRLRDGYHATVGQILRFKIPSLAAFVLIVAAMVYFFQKMPT
                     AYLPDEDQGMLMAMAQLPVGSTREQTDEVLSEVRRHFLEDEKEAVESIMTVSGFSFAG
                     RGQNQAMAFVKLKDWDLRDREDLRAPAVAGRALGAFSKLRNAVAFAFPPPAVVELGTA
                     TGFDFMLQDRGGLGHEKLMEARRQVMTLAMKDPRLTRVRPNGLDDVPEYHGDVDWEKA
                     GTWGVPIGNIHQTISAAFGSAYVNDFIQGGRVKRVYVQADAPYRMLPGDLERLYVRNN
                     KGAMVPVSALASGKWVYDSPRLERFNSFPALNFQGEPAPGRSSGEAMQAMEEIVAKLP
                     QGIGHDWTGVSYQERAGQAQTGLLYSFSVLVIFLVLAALYESWTVPISIILALPLGVI
                     GGVLASSLRGLPNDVYFQIGLLTVLGLTTKNAILIVQFAKIRVEQGAGLIEATLEAAK
                     LRLRPIVMTSLAFGFGVLPLALARGAGAGAQQAIGTSVLGGMITATFLAIFFIPLFFV
                     MVVQLFERRKRRRIEAEEL"
     gene            8832..10241
                     /locus_tag="VFQ79_00285"
     CDS             8832..10241
                     /locus_tag="VFQ79_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0138110.1"
                     /translation="MYRKLLLLGMAFFLCGCLRPGAYHRPAPPVPNSWPEGAAAQAGA
                     PDVPEAASVKWQEFFTDRGLQSVIELALANNRDLRIATLNIERVQALYRIQRAEQFPT
                     VSVSAAGDLYRLPKSAGFAGLIVPEAVTVQQYNINLGAASWELDLFGRVRSLKSAALE
                     RFLATEQARSATQIALVAAVANSYLALAADRENLRLAQDTLATQQASYELILRTREAG
                     MASDLDVRQAQSQVEAARVDIARYTGQVELDENALNLLAGTTVPANLLPGDLDSAGSL
                     KDIGAGLPSEVLLRRPDILMAEHQLKAAYANIGAARAAFFPRIALTAGAGLMSADLSD
                     LFKFGAKTWNFAPQVALPIFDAGARKANLKVTEVDREAATAEYEKAIQSAFREVSDSL
                     SLRTTLMEQQSAQQELVKTVEAAYRLSEARYKAGIDSYLSVLVAQRLMYSAQQGLIGL
                     RMARLGNLVTLYKVLGGGA"
     gene            10265..11023
                     /locus_tag="VFQ79_00290"
     CDS             10265..11023
                     /locus_tag="VFQ79_00290"
                     /inference="COORDINATES: protein motif:HMM:NF040426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6454 family protein"
                     /protein_id="HEU0138111.1"
                     /translation="MRFLLAVLPLSVMAADHVGDLRPLQTVELHAETHHVQGIDVDRD
                     TLWVSAVDTRGRRGLLFRFDRKTGKLLHSAEVHSGKRYHPGGISIEGDSVWVPVAEYR
                     RSSTTSMQKRHRETFALQAEFDINDHIGAVAVVPEGLVGANWDARELYVWTKSGKRLR
                     RVVNPAGIAIQDMKFVNGRLIAGGLERDGRGAVIWLEWPGLKELRRIHVGRTDRGVAF
                     THEGLAVHGRTLYLLPEDGPSRLFRFEIPDTVDQ"
     gene            complement(11049..11696)
                     /gene="alkB"
                     /locus_tag="VFQ79_00295"
     CDS             complement(11049..11696)
                     /gene="alkB"
                     /locus_tag="VFQ79_00295"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: protein motif:HMM:NF011930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEU0138112.1"
                     /translation="MTVLFDSNSLPPSLEPLEEGAVLLRGFAASEAPMLIDEVERIAQ
                     AAPFRHLVTPGGYTMSVAMTNCGRVGWVSDRTGYRYDPVDPATGAPWPAMPVAFLDLA
                     VRAAAQGGFAGYDPDACLINRYVAGTKLGLHQDRDEKDAWAPIVSVSLGLPAVFLWGG
                     KRRSDPVRRLRVENGDVAVWGGPARFVYHGVAPLKDGQHPLTGASRINLTFRKVF"
     gene            complement(11716..12117)
                     /locus_tag="VFQ79_00300"
     CDS             complement(11716..12117)
                     /locus_tag="VFQ79_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU0138113.1"
                     /translation="MTGRNGPSDRVRIDKWLWAARFFKTRSLAARACDLGRIEANGQQ
                     CKPSREVRPGDLLRVKNDAGDFQVEILALSQMRGPAAVAQTLYKETDASRESRRQLAE
                     ERKAMPYFDAFEEGRPSKRDRRQIDRLRGRV"
     gene            complement(12092..13333)
                     /locus_tag="VFQ79_00305"
     CDS             complement(12092..13333)
                     /locus_tag="VFQ79_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0138114.1"
                     /translation="MKKTLLPLFVLASALHAQTYDLLLKGGHVIDPKNKVNAPMDVAV
                     AGGKIARIATGIPASQARRTVDVSGLTVTPGLIDIHVHVYPRPENKDVARDSNVQADA
                     HTFRSGVTTVVDAGTSGANNFADFRARVVDRAQTRVLALLNIAAAGMGTGKEDEVSGL
                     DSQTAARVAKANRDVVVGFKSAHFAGPGWESIEAAVKAGTEADMPVMVDFGYLNQIRN
                     LPTLLLDKLRPGDIYTHCYSGHREELLPDGKINPAMFTARKRGIIFDVGFGAGSFYWY
                     VAVPGFEQGFPPDSISTDLHTGSMNSGMKTMTNVMSAILNQGSTLEEVIRMSTWNPAR
                     QIKRQELGNLDVGSEADITVLRTLTGDFGFIDSAGAVNSGGQRLIAELTLRKGRVVWD
                     LNGRAAESWKTFKYDRKKWTK"
     gene            complement(13358..16564)
                     /locus_tag="VFQ79_00310"
     CDS             complement(13358..16564)
                     /locus_tag="VFQ79_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138115.1"
                     /translation="MHQTHLGFVLLLGLALPPAGFAQSGESTQILGIVEDQSGAVVPA
                     VAISATHVATGQVRRVVTGESGTYVFPFMAPGEYIVRAERDGFKADVRTGLILQLNQK
                     ARVDFQLQVGAVSEAVEVSARGVILNTDDATMGNVVEQRRVVDLPLNGRNFANLAGLM
                     PGVIKGISSNTNQYGRRDTAIAISANGMRENQGQVLYDGVSTAWNINNATFFRASIEA
                     IQEFKVHAGTYSAEYGHNAGAQVEILTRPGTNQVHGALFEFLRNDNFDARNYFRPRPL
                     SKDVLQRNQFGFVLSGPVWIPKLYNGRDRTFWMVNYEGQREKQEVASVASVVPTAFRS
                     SDFTSLATPLRDPLGGTFAGNVLPESRISPISRRYLEIHPQPNLPGTGANLAGVDQQL
                     NNVNQVFARGDHNIGSNDKLFARVAVFNYNFPTIPINYFSTIDSRITARNAVLSHTHI
                     FSPTVINEARLGFNRNWILRKNPRTNTNFDPESVGLQGIRAGAGAGQSRPLSPLETGY
                     VPMAISGYLTMGDGDLLPDFNVSETWQIVDSITWTRGVHTFKAGIDFRRLRMDRAGAN
                     VPRGQFQFNGQVTGNAVADFLIGFPSQSQSPEGILPVEFRQHTYAWYVQDEWKATRKL
                     TLNLGLRYDYVGTVDEINGIQRALRLDRPGGYLYPESSATEPREPARFYNAEKNRFWP
                     RLGLAYRPADGWVIRMGGGVFNNANQMNNLTVFSDPERRASITFVADPQNPTALTFDN
                     PFPIALEAAAPPINVVYIPPDRVNAYNVQWSASVQRQLSESTALELAYVGSQASHLDN
                     SRNLNDALPGTGAFQPRRPFPRWGAIRYLASDGKSYYQSMQVRAERRFSRGLSFLAGY
                     TWAHNIDQAYGTNESLPFTPGGVQNQNCFACERANSGFDYRHRFTTSFLWSVPTPASW
                     RGALRFALANWSFNGILTYQTGFPFTVTQQGNRQGTGSATQRPDYVSGQSPKLSNPDP
                     SRWFNTDAFQFAELKYGAVGRNTLRQPGMKVWDIGLFKEFPITEGHRFQFRWEAFNLW
                     NTPQFRAPNAQLGGPSFGQITSTWLDNRQMQFALKYLF"
     gene            16774..>17428
                     /locus_tag="VFQ79_00315"
     CDS             16774..>17428
                     /locus_tag="VFQ79_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0138116.1"
                     /translation="MTRLIRRRWISFPFIAVLFCCAGAFAQEYRGRVQGTVTDPSQAS
                     VAGASVTLRNVNTGVETSRQTEANGAYLFDFVQPGSYSITVQAAGFQRFVQENINVLT
                     RGDVTVNATLSVGGVTETVTVTEEVAQVQFNTSTMTTTVQGSMLKDIPILARNPFTLA
                     MLNPAVINKYWDVSHRNPFYMWSSNGLDIGGRTGGKNDMLLDGVSLGLSARGSYMPSM
                     "
BASE COUNT         3494 a         5071 c         5347 g         3516 t
ORIGIN      
        1 tgatcgacag gctgtccgac gccggcgtcg cgacattcaa cctggccgtc gattccgttg
       61 aagaccgcga agaactgccc aaggccctca acccgatccg gcgcgagttc gagtatctca
      121 tcaccaagca atacgtctac gattacagcg tcttcttcaa catcaacatc acacgcatca
      181 atctggagga cgtcaaagcg ctgaccgaga tagctcacga tgtggggata gcaaccgact
      241 atcacatcaa cgaggcgccg atgctcgatc aacctcactt caagcatacg gatgcgaacg
      301 aaaccttcat tactccggag gactggcccc aggtcgactc ggtgatcgat tggcttatcg
      361 aaaagtcccg ttcggggttc aagatggtta attccatccg gcgcctggag gagatgaagc
      421 agttcatgcg gggtaagttg caggactgga attgccgcgc cggccaaaac tcgttgatca
      481 tccggacgga tggcacgctg gcgccgtgtt tccccatgta ttccgcatcg tacgactggg
      541 gagtggcaag ccaacccagc ttcaacatcc atcaactgca agacatgaag agagagtgcc
      601 agccgcactg cttctcaacg ctgaatcaca acctcgcgta ctgttacgat gccgggcgtg
      661 ctttcaagtg ggtattgaaa caggcgctgc gcggttttag aggaactaca ggcggctttg
      721 acgagtgagg ctggagatcg attgcgagcc cggcggcgat gaggtggagg ctttgttaac
      781 ggaagatcag cgtctgcaca tcagaaatgc gctcgaagcc aggcgtcgcg aactgatcga
      841 tggcattcgc agccaagccg tggaacttgc ggtcagcgat ggcattcacg accccatcga
      901 taaagtccag atgatggcct atcgcgatga ggcggtccaa caggtgggac ggctctccga
      961 aactttatcg agcgtggaga agtcactcgc agcgctgtcc ggcggctact atggccattg
     1021 cgccgagtgt ggtaagccaa tcgcggcaaa gcggttggag gtcattccgt gggcaagcca
     1081 ctgcctcaat tgccaggcgc gaagggagtc caaccatcat gccggtgctg gaccttcggg
     1141 cgacgagcgt gaggcggcgg cataaccggc cttggccttt ccggccgatt tactccttgc
     1201 tttgtcccga cagaagcgct gaggccgcct gcgcgacgcc ggcggcgaat tgctgcgcca
     1261 gcgcggtggc gctttccgag gtgtgtcgaa gcgtggcaag ccagaaacca taggccagac
     1321 atgcattgac agcgtaaggg acgagatgaa ttgagaggat aacccgcgaa cgcgaatctg
     1381 gaaagtgtag agcgagttcg cattgccact tgattccgac agagccgacc tcattgcgat
     1441 gaaggtcggc ggcatattcg ggagcctcca gaagagccca gttcgtcagc actgcattag
     1501 acgcgccgga aacgcaaacg gacacagtcg cctccgccat gctctctatg cactctaacg
     1561 ggcttttccg gccgatcgaa gccaaacgat cgtcgagcgc ccgcaggcgt gtctcgatgt
     1621 tgttttcaac ggcatccctg aacagacgct ccttgctgtt gaagtgaacg tatagcacag
     1681 gctccgagac tcccgccgct ttggcgagcg cttgcgtcgt ggtcccgtgc agacctgtcc
     1741 gggcaaactg cgctgccgca atctcgaaaa tctgtgagcg ccggtctgcg ctcgcgaggc
     1801 gcttggattc tctcccgcaa tcagccatcg gcgtccttgg taggaatcaa cccctgcaca
     1861 atgtaggaag tataaacgcc actggcgagc agtcgaacgc ccttggccgt gagacgcctc
     1921 agcaaggcgc ggccggcctc gtcgattatc gtgaggtcgc gcaggaagag agcaaccggc
     1981 ttgccggtcg atatctcctg gctgcaatac gtctcaacga actcgatgca atcgtgggag
     2041 agtttcccgt cgatggtgat gaccgtgccc gactgctctt ctgtcctgcc gacatggaac
     2101 atggtgatct atctctcgtc aggttccgat gcacgctgct atccaattta cttacgacct
     2161 tggaagctga attgcgatca tccagttgat cctaacgcgg aatgggcggg tttgaaggag
     2221 catcatgccg acgatcgaca tctgccaacg tcaattgccg aaatcggcgc cccctggtag
     2281 cattctggat atgggaatcc cagattcgaa gatctacaaa gcgcttttcg atctttctca
     2341 atcgatcgcc gggcacaccg atctggagac tctctgcaac tcgctggcag actcgctacg
     2401 gcacgtcgtt tcgttcgatt ttctggcgct ggttcttcac gatcctgtcc gcgatcagct
     2461 ccgtttgcat gcgatcagca caagccggcc ctacaaaaac catagggaag tcgtccttcc
     2521 cgcggatggc gaccatttcg gcgcgctggt ctggcgcgat cagaaaccgc tggtgttatc
     2581 gccgctctac aaagagactc aataccgcga tgtggtggac gaagcgctgc aagcggggat
     2641 tcaggcgatc attctggtcc ctctttcaaa cggcgaccgc cggctcggga tcctagggtt
     2701 cggatttacc gcacccttcc agcctgacga aggcgcagtg gcattcctac agcgggttgc
     2761 atcggaagtc gcggtttccg tcgagggcta tctgacccgg cacgcgcttt tacacgagcg
     2821 cgaccggata cgggtgttgt tcgagatcac caatgccctg gtttccaagc tcccgctgga
     2881 agagctcttc tcggcgatct ccgaacagat cagccgcgtc gttgagcacg attttgcagt
     2941 cgtgggacta ctggacaaag acacaggaag aatccacctt agcgggctac attcacccgg
     3001 tggcattcag gtcgagatcg aggagacatc gggcgatccg gagggacttc ctccgggcga
     3061 agcattggcc accgggaaac cagtcgtgat aaccgaaccc gacttcgaac gttttcggtc
     3121 tccgcgttac cgcaagtact tcgagatggg cttccgttcg ggctgtgcga tcccgtttac
     3181 cggcgtaaac gggatctccg gcgtgctcag cctggcccgc aaaagtggag agccgtttac
     3241 gaacgacgaa gtggacctat tggttcaggt ggcacgccag attgccattg cgacagagaa
     3301 ttcactcgcc taccgcgagc ttttgcagat caaggataaa ctggccaccg aaaaactgta
     3361 tctcgaagat gagatccgca ccgatcagaa cctgggcaac atgatcgggg aaagcccggc
     3421 ctttcaagcc gtgctgagtg ccattcaggt cgtggccccg acggacgcca cggtgctcat
     3481 cgagggcgaa acggggaccg gaaaggaact ggtcgctcgc gctttgcacg acttgagcgg
     3541 acggagccag cgctccttca tcaaagtgaa ttgcgcggcc attcccgcaa cgttgctcga
     3601 aagcgaattg ttcggccacg agaagggctc gttcaccggc gcgttcgcgc agaagatcgg
     3661 ccggtttgag ctggcgcatc aggggacgct gttcctggac gaggtcggag agatcccgct
     3721 ggaactgcaa tccaagctgc tgcgcgccat ccaggaacag gaactggagc gtctgggcgg
     3781 caaccggacc atccgggtgg atgtccgttt tattgccgcg acaaaccgca gtcttaagca
     3841 gatggtggac gaaggcaagt tccgcagcga cctttactac cggctgcatg tctttccttt
     3901 gatggttcct cccctgcggg agcgaaagga agatattcct ctcctgattc gttactttac
     3961 acagaagtat gcgaagcgga tgaaccgtcc gattgaatcg gttccttcgg ctgccatcga
     4021 agccttgacc aactacgaat ggccgggcaa catccgcgaa ttgcagaacg tgattgagag
     4081 atccgtcatc cttagtcctg gccgcgtgct tcagatcgcg attccggcga ttgcggaacc
     4141 atcctctctt gccgccagaa caccccggct tgaagaatcc gccgagcgcg aacggatcct
     4201 ccgggctttg cacgaatccc atgggaaagt ggcggggcca aacggggccg cggcccgcct
     4261 tggcttccgg cgtacgactc tgcaatcccg aatgaagaaa ctcggcattg agcgccagta
     4321 ccgctgactt cggcaattga ctggcaaatg ccgaagttga cgaagcgatc ttcggcagta
     4381 cacccgtaag tctttttatc taaattcacc ctcttggcgg ctggcatgcg tttcctatat
     4441 ttcgagacaa aaatgcgaaa tgagcatctt gttgtaaccg gcctcgttct ctcggtcctg
     4501 gtctcctcga gttgtaagcc caaggcaagc gccgtaccct cccaggcagg ccctgccgaa
     4561 gtcgcaaccg tcacgatccg gcccgaacga acggtgttga ccactgaatt acccgggcgg
     4621 acctccgcct atcttgttgc tgaaatccgc ccccaggtaa atggccttat tcagaaccgc
     4681 cttttccggg aaggttccaa tgtcaaggcc ggcgatctgc tctatcagat cgatcccgcg
     4741 ccctatcaag ctgcctacga tcaagccaag gccgctctcg tcacggctga agcggatctc
     4801 gtcacggccg aggcaaatct cccggccatc cgttcccgcg cagagcgttt aaaagggctg
     4861 gtggccattc acgcggtctc acagcaggat tacgacgacg ccagcgcggc tttgcgacag
     4921 gccgaggcca gtctggcggc acgcaaggcc tctgttgaga tcaatcgggc agccgtggag
     4981 agcgcccgta tcaatctctc ctttactccc atcaaggcgc ccatctcagg acgcatcggc
     5041 atatcgagta tcacggccgg cgcgctggct accgcctatc agccaacccc actggcggtt
     5101 atacagcagt tggaccccat ctacgtggat gtggtgcaag ccaatgcgga actgctgcgc
     5161 ttgcggcagc gattggaaag cggacggctc aaacgcaatg gaacggttca gaacaaggtg
     5221 aagctgttcc tggaagacaa aacgccctat cctctggcgg gcacgctgca atttcaggat
     5281 gtcacggtag acccggccac cggctcggtc acgctgcggc tggtttttcc caacccaaaa
     5341 caggtgcttc taccgggcat gtttgtgcgt gcggtcgtac aggagggggt gagcgaacag
     5401 gcccttctcg tccctcagca gggcgtcacc cgcaacacca agggcattcc cgttgcctgg
     5461 gttgtgggac aggacgaaaa ggttgaggag cggattctcg aactggaccg ggccatcggt
     5521 gacaaatggc tggtcaccag cgggctggtc gccggtgacc ggctgattgt cgaggggttc
     5581 cagaaggtgc gtccgggtgc ccaggttcgg gcggttccct ttgacgcaaa cggcagtccc
     5641 gcggcatccg gaggccagga caaggcgaac ggaggaagcg gtgtctaaat ttttcctcca
     5701 ccggcccgtc tttgcgtggg tcatcgccat cgggatgatg gtcggcggcg ccctcgccat
     5761 ctacaacctg cccatctcgc aatatccgcc catcgcaccg ccgtcgatct cgattcgtgc
     5821 cgtctatccc ggcgcgacgg caaagaccgt ggaagacagc gtggtccaga tcatcgagca
     5881 gaaaatgacg ggcctcgaca ggatgctcta catctccgcc accagcgact cgtcgggcag
     5941 cggcgtgctg gagttgacct tcgctcccgg caccgacccc gatctggcat ggtccaaggt
     6001 acagaacaag ctgcaactcg caatgccgat gctgcccgag gtggtgcagc ggcagggcgt
     6061 cacggtggcg aaatcgaccc gcaactacct gattatggtt ggcatcgcgt cgacgaccga
     6121 caacatggat ctgtacgatc tgaacgacta cgcagtgtcg cagatccagc cgtctctggc
     6181 gcgcgtgccc ggcgtcggcg aagtggaggc gttcggctcg caatactcca tgcgcgtttg
     6241 gatggatcca aacaagctca cgcagtacaa catgacggtc agcgatatta tcgcggcgat
     6301 tcgtacgtac aacgtgcagg tgtcggccgg acaattcggc gggatgccgg ccgtccaggg
     6361 acagcggctg aacgcctcga ttctcgtcca gtccttgctg aagacacccg aggaattcgg
     6421 cgcgattccg ctgcgcaaca accccgacgg atcgatcgtt cgcgttcgcg acatcgcccg
     6481 tacggagttg ggaacggagt tctacgacgt acaggcctct tataacggaa ggccggcggc
     6541 tgtgctgccc gttcgccagg agccgggcgc caacgcgctc gataccgcgg cggcggtaaa
     6601 agccaagatg gaagagttgt cgcagtactt cccccccggg gttcaggtga tctacccgta
     6661 cgacacgacg cctttcgtgc gtgtcgctat cggtgaagtg gtgagaacgc tattcgagac
     6721 gatcttcctc gtgttcctca tcatgtacct gtttctgggc aacatgcgcg cgacgctggt
     6781 gcccaccatc gccgtgccgg tggttatcct cggaacgttt ggcgtattgg cgctgttcgg
     6841 cttctccatc aacatgctga cgatgttcgc catggtgctt gccataggct tgctcgtgga
     6901 cgacgccatc gttgtggtgg agaacgtgga gcgcgtgatg agcgaagagg ggctctcacc
     6961 atgggaggcc acgatgaagt ccatggacga gatcaccggc gcgctggtcg gtatcggatg
     7021 cgtcctgtcg gccgttttcg cgccgatgat cttcttcccg ggttccacgg gcgttatcta
     7081 tcgacagttc tcggtgaccg taattgcgtc catgctccta tccgtcgttg tcgctcttgt
     7141 cctgactccg gttctgtgcg catctctgct gaagcctgta ccgaaggggc acgaagccgc
     7201 ggagaccggc tttcgcctgt tgcggccgtt cttcctcgcc ttcgatcgcg cgttcaaccg
     7261 tctgcgcgac ggctatcatg ccacggtggg acaaatcctc cggttcaaga tcccatcgct
     7321 cgcggccttc gtcttgattg tagcggcgat ggtttatttc ttccagaaga tgccaacggc
     7381 gtacctgccc gatgaagacc agggaatgct gatggcgatg gctcaactgc cggtcggctc
     7441 cactcgcgag cagaccgacg aggtgctgtc cgaagtccgc cgacactttc tcgaagacga
     7501 gaaggaggcc gtggagtcta tcatgaccgt gtccggcttc agctttgccg gccgcggcca
     7561 gaaccaggcc atggccttcg tcaagctgaa ggattgggat ctgcgcgatc gtgaagatct
     7621 gcgcgcgccc gccgtcgccg gaagagcgct gggcgctttc tccaaactgc gcaacgccgt
     7681 cgcctttgca ttcccgcccc cggccgtcgt ggagttgggc accgccaccg gattcgactt
     7741 catgctgcag gatcgcggcg gcctcggtca cgagaagctg atggaggcgc gcaggcaggt
     7801 gatgacgctc gccatgaagg atccgcgctt gacgcgcgtc cgtccgaacg gcctggatga
     7861 tgtgcccgag tatcacggcg acgtcgattg ggaaaaagcc ggcacgtggg gcgttcccat
     7921 cggtaacatt caccagacga tatcggctgc ctttggcagc gcctatgtca acgacttcat
     7981 tcagggcgga cgagtgaaac gcgtgtatgt acaggccgac gcgccgtacc gtatgcttcc
     8041 tggcgacctg gagcggctgt acgtccgcaa caacaagggc gcgatggtgc ctgttagcgc
     8101 tcttgcatcc ggcaaatggg tgtacgactc tccgcgcctg gaaaggttca acagcttccc
     8161 cgcgctcaat ttccagggcg agcccgcgcc gggtagaagc tccggcgagg cgatgcaggc
     8221 gatggaagag atcgtggcca agctgccgca gggcatcggt cacgactgga ccggcgtctc
     8281 ttaccaggaa cgtgccggcc aggcgcagac cgggctcttg tactcctttt cggttctggt
     8341 catcttcctc gtattggcgg cgctttacga gagttggacg gtcccgattt cgatcatatt
     8401 ggccctgcct ctcggagtga tcggaggcgt gctggcttct tccctccggg gattgcccaa
     8461 tgacgtctat tttcagatcg gattgcttac ggtcctgggg ttgacgacta aaaacgccat
     8521 tctgatcgtt cagttcgcca agattcgcgt ggagcaggga gcgggcttga ttgaggcgac
     8581 gctcgaggcg gccaagctcc ggctccgccc gatcgtgatg acgtcgctcg cgttcggttt
     8641 cggcgttctg cctttggcgc ttgccagagg ggcgggagcg ggggctcaac aggccatcgg
     8701 caccagcgtt ttgggtggga tgattacagc caccttcctg gcaatctttt ttatccccct
     8761 gttttttgtg atggtggtgc aactgttcga aagaaggaag cgccggcgca tcgaggcgga
     8821 ggagctatag tatgtatcga aaacttcttc ttctggggat ggctttcttc ctgtgcgggt
     8881 gtctacgccc cggagcatat caccggcctg cgccgccggt gccgaattcc tggccggagg
     8941 gtgccgccgc ccaagccggc gcgccggatg tgcccgaagc cgccagcgtg aagtggcagg
     9001 aatttttcac cgatcgcggg cttcaatctg tgattgagct ggctctggct aataatcgcg
     9061 acctgcgaat agccacgctc aacatcgaga gagttcaggc cttgtaccgg atccaacgtg
     9121 ccgaacagtt ccccacggtt agcgtatccg ccgccggaga tctctaccgg cttcccaaaa
     9181 gcgcggggtt cgccgggttg atcgttccgg aggccgtgac ggtccaacaa tacaacataa
     9241 atctgggcgc tgcttcctgg gaactcgacc tcttcggacg tgttcgcagc ttgaagagcg
     9301 cggcgctcga acgatttctg gcgacagagc aggcccgttc cgcgacgcag atcgcgctgg
     9361 tggccgcggt agcgaacagc tatcttgccc tggccgccga tcgcgagaac ctgcgtctgg
     9421 cccaggacac tctcgctaca cagcaggctt cctacgaact gatcctgcgc acacgagagg
     9481 cgggcatggc ctctgacctg gacgtccgcc aggcgcagag ccaggtggag gcggcacgag
     9541 tggatatcgc ccgctacacc ggccaggtgg aattggacga gaatgcgctc aaccttttgg
     9601 caggcacaac ggttccggcc aatctgctgc ccggcgacct ggattctgct ggctcgctaa
     9661 aggatatcgg tgccggatta ccttcggaag tgctgctccg gcgcccggac attctcatgg
     9721 cggagcacca gttgaaagcg gcgtacgcca acatcggggc agcccgcgcg gcattcttcc
     9781 cgcgaatcgc cctgacggcc ggcgccggtc tcatgagcgc cgatctatcc gacttgttca
     9841 aattcggagc caaaacatgg aactttgcgc cgcaagttgc attgcccatc ttcgatgcag
     9901 gcgctcgcaa ggcgaatctc aaggtgactg aggtagaccg ggaagcagct accgcggaat
     9961 atgagaaggc gattcaatcg gccttccgcg aagtgagcga ctccctgagc ctgaggacca
    10021 cgctgatgga acagcagagc gcacagcagg agttggtgaa gacagtggag gcggcctacc
    10081 ggctttcaga ggctcgttac aaagcgggga tcgacagcta cctgagcgtc ctcgttgctc
    10141 aacgattgat gtatagcgcg caacagggat tgatcggcct ccgcatggct cgtctgggga
    10201 atctggttac cctttacaaa gtgctgggcg gaggagcgta ggatggtcgt atcaaagaga
    10261 gcaaatgaga ttcctcctcg ccgtacttcc gctttctgtg atggccgcgg accacgtggg
    10321 cgatctgcgg cccctgcaaa ctgtcgagtt gcatgctgaa acgcaccatg tgcaaggcat
    10381 cgatgtagat cgcgacaccc tgtgggtatc tgccgtggat acgcgaggcc ggcgtggact
    10441 gcttttccgg tttgatcgta agaccggcaa gctgcttcac tccgccgaag tgcacagcgg
    10501 caagcgctat catccgggag gcatcagcat agagggcgat agcgtgtggg tgccggtcgc
    10561 ggagtatcgc cgttccagca cgaccagtat gcagaaacgc caccgcgaga cgttcgcttt
    10621 gcaagccgaa tttgacatca acgaccatat aggagccgtc gctgtcgtgc cggaaggtct
    10681 ggtgggcgcc aattgggacg cacgcgagct ttacgtctgg acgaagtccg gcaaacggtt
    10741 gcggcgcgtg gtgaatccgg cgggcatcgc gatccaggat atgaagttcg tcaatgggcg
    10801 cctgatcgca ggcggcctgg aacgcgacgg ccgcggagcc gtgatctggc tcgaatggcc
    10861 tggactaaaa gagttgcgga gaatacatgt gggacgcacg gaccgcggcg tagcgtttac
    10921 tcacgaaggt ctcgctgtcc acgggcgcac gctgtacctg ctaccggaag acggaccaag
    10981 ccggctcttc cgtttcgaga ttccggacac cgtcgatcag tagaatcagg acggcctggc
    11041 cggtacgatc agaatacttt gcggaatgtg agattgatgc gggaagctcc ggtgagcgga
    11101 tgttgtccat ccttcaacgg ggcgacaccg tgatagacga aacgggcggg tccgccccac
    11161 accgcaacat cgccattctc cacgcgcagc cggcgcaccg gatcggagcg gcgcttgccg
    11221 ccccacagga atacggcagg caggcctaac gatacggaga cgataggtgc ccaggcgtcc
    11281 ttctcgtcac gatcctgatg caggccgagc ttggtgcctg cgacatagcg gttgatgaga
    11341 cacgcatcgg gatcgtagcc cgcgaacccg ccctgcgcgg cggcgcgcac tgccaggtcc
    11401 agaaatgcca ccggcatcgc cggccatggt gctccggtag cgggatcaac cgggtcataa
    11461 cggtagccgg tgcggtcgga tacccacccg acgcggccgc aattggtcat ggcgacggac
    11521 atggtataac cgccgggcgt gaccagatgg cggaacggcg ctgcctgcgc gatccgctcc
    11581 acttcgtcga tcagcatcgg cgcttcggat gcagcgaagc cgcgcagaag cacggcgcct
    11641 tcttccagag gctccaggga aggcggcagt gaattgctgt cgaacagaac ggtcatacag
    11701 cgtcccgttc cggcctcaca ctctaccccg caatcgatcg atctggcggc ggtcgcgttt
    11761 cgacggccgt ccttcctcga aggcgtcgaa gtacggcatc gctttgcgct cttccgccaa
    11821 ctgcctccgc gactcccggc tcgcatcggt ctctttgtaa agagtctgtg caaccgcagc
    11881 cgggccgcgc atctggctga gggccaggat ctccacttga aaatcgcccg cgtcgttttt
    11941 tactcgcagc aggtcgccgg gccggacttc ccgtgaaggc ttgcattgct gtccattcgc
    12001 ctcgatcctg ccgagatcac acgcccgcgc agcgagtgat cgcgtcttga agaaccgggc
    12061 ggcccagagc cacttgtcaa ttcggacccg gtcacttggt ccatttcttc cggtcatact
    12121 tgaaggtctt ccagctttca gcggcgcggc cattaaggtc ccacaccacg cggcccttgc
    12181 gcagagtcaa ctccgcgata agccgctgtc cgccggaatt aacggcgccg gcggagtcga
    12241 tgaagccgaa atcgccggtt aacgtccgga gaacggtaat gtccgcctcg gagccgacat
    12301 cgaggttgcc gagttcctga cgcttgatct ggcgcgccgg attccatgtg gacatgcgga
    12361 tcacttcttc gagtgtcgaa ccctggttga ggatcgccga catgacattg gtcatggtct
    12421 tcatccccga attcatacta ccagtatgca ggtcggtcga aatggaatcc ggcgggaatc
    12481 cctgctcgaa accgggcacg gcgacgtacc agtagaaact gccggcgccg aagccgacat
    12541 cgaagatgat gccgcgtttg cgggcggtga acatagccgg gttgatcttt ccgtcgggca
    12601 gcagttcctc gcgatgcccc gagtagcagt gagtgtagat atcgccgggc ctgagcttgt
    12661 cgagcagcag cgttggcaga ttgcgaatct gattcaagta tccgaagtcc accataaccg
    12721 gcatgtccgc ctcagtgccc gctttcaccg cggcttcgat ggattcccaa cccggaccgg
    12781 cgaagtgagc ggacttgaat ccgacaacga cgtcgcggtt cgctttggca acgcgggccg
    12841 cggtctgcga atcaagaccg gacacctcgt cttcttttcc agtacccatg ccggcggcgg
    12901 cgatattgag cagcgcgagg acgcgcgtct gcgccctatc gacgacgcgc gcgcggaaat
    12961 cggcgaagtt gttagcgccc gacgtgccgg catcgaccac ggtggtaacg ccgctgcgga
    13021 acgtgtgggc atcggcctgg acgttgctgt cccgcgcgac atctttattc tccggacgcg
    13081 gatagacgtg gacgtggatg tcgatgagac ccggcgtgac ggtaaggccc gacacgtcca
    13141 cggtccttct agcctgcgat gccgggatgc cggtcgcgat acgcgcaatc ttgcctccgg
    13201 cgacggcgac gtccatcggc gcgttgacct tgtttttggg atcgatgacg tggccgcctt
    13261 tgagcagcag atcgtacgtc tgcgcatgga gtgcggaagc caaaacaaac agtggcaata
    13321 atgtcttctt cataaagcga tccattcctc gaagttttca gaataagtac ttcaacgcaa
    13381 attgcatctg gcgattgtca agccaggtgg acgtgatctg accaaacgaa ggaccgccga
    13441 gttgtgcgtt gggagcgcgg aactgcggcg tattccagag gttgaaagct tcccaacgga
    13501 actgaaaccg gtgaccttcg gttatgggaa actccttgaa caacccgatg tcccaaactt
    13561 tcataccggg ctggcgcagc gtgttgcggc cgacggctcc gtacttcagc tcggcgaact
    13621 gaaatgcgtc cgtgttgaac cagcgggagg gatcgggatt gctcagcttc ggactctggc
    13681 cggaaacata gtcgggccgc tgtgtagcgg agccggtgcc ttgccggttg ccttgctgtg
    13741 tgacggtgaa cggaaatccg gtctggtaag tgagaatgcc attgaacgac cagttcgcca
    13801 gtgcaaaccg cagagcgccg cgccagcttg ccggagtggg aacgctccac agaaagctgg
    13861 tggtgaagcg atgacggtag tcgaatcccg agttcgcacg ctcgcaagca aagcagttct
    13921 ggttctgaac gcctccgggg gtgaacggca gcgactcgtt tgtaccgtaa gcctggtcga
    13981 tattgtgtgc ccaggtgtaa ccggccagga acgagagacc acggctgaaa cgccgctccg
    14041 ctcgcacctg catggattgg taatacgact tgccgtcgct ggcaagatag cggatggctc
    14101 cccagcgcgg gaaaggccgc cgcggctgga aggcgccggt cccgggtagc gcgtcattca
    14161 gatttcggct gttatccaga tgcgaagcct ggctgccgac ataggccagc tcgagcgcgg
    14221 tggattctga aagctgccgc tgcacggagg cgctccactg gacgttataa gcgttcacgc
    14281 gatccggcgg aatgtaaacg acgttgatag gcggggcagc cgcttcgagg gcgatgggga
    14341 agggattgtc aaaagtcagc gcggtggggt tctgcgggtc ggcgacaaac gtgatggaag
    14401 ctcgccgctc ggggtccgaa aacacggtga gattgttcat ctggttcgca ttattgaata
    14461 cgccgccgcc catgcgtatg acccatccgt cagcgggccg gtaggcaagc cccagccgcg
    14521 gccagaagcg atttttctcc gcgttataaa agcgagccgg ctcgcgtggt tcggtggcgg
    14581 acgactcggg ataaagatat ccgccaggac ggtcgagacg caacgcgcgt tgaattccgt
    14641 tgatctcgtc cacggtgccg acatagtcgt atcgcaaacc cagattgagt gtgagcttgc
    14701 gagtggcctt ccattcgtcc tggacatacc aggcgtaagt gtgctggcgg aattcgacgg
    14761 gaagaatccc ttccggcgac tgcgattgcg acgggaagcc gatcagaaag tctgcaacgg
    14821 cgttgcctgt gacctgcccg ttgaattgaa attgcccgcg gggcacgttg gctccggcgc
    14881 ggtccatgcg aagacggcgg aaatcgattc cggctttgaa ggtgtgaact ccgcgagtcc
    14941 aagtaatgct gtctacgatc tgccaggttt cactgacatt gaaatccggg agcaggtcgc
    15001 cgtcgcccat cgtgagatag ccggaaatgg ccatcggcac gtaaccggtc tccagcggag
    15061 aaagcggccg cgactgcccc gctccggctc cggcacggat gccctggagt cctaccgatt
    15121 cggggtcgaa gttcgtgttc gtgcgcgggt ttttgcgaag tatccagttc cggttgaacc
    15181 cgagccgggc ctcattgatc acggtggggc tgaagatgtg cgtatggctc agcacggcgt
    15241 tgcgggcggt gatgcggctg tcgatggtgg agaagtaatt gatggggatc gttgggaaat
    15301 tgtagttgaa cacggcgact cgggcgaaca gtttgtcgtt ggagccgata ttgtgatcgc
    15361 ctcgtgcaaa cacctggttc acgttattga gctgctgatc gacgccggcc aggttggcgc
    15421 cggtaccggg gagattcggc tgcgggtgga tttcgagata gcggcgtgag atggggctga
    15481 tgcggctctc cggaagaacg ttgccggcga acgtcccgcc aagcgggtcg cgcaacggag
    15541 tggcaagcga ggtaaagtcg ctgcttcgga aagcggtggg aaccaccgac gccacggagg
    15601 ccacttcctg tttttcgcgc tgtccctcgt agttcaccat ccagaaggtg cggtcgcggc
    15661 cgttgtagag cttcggaatc cagaccgggc cgctcagcac aaacccaaac tggttacgtt
    15721 gcaggacgtc tttgctgaga ggccggggac ggaagtaatt gcgggcatcg aaattgtcgt
    15781 tgcgcagaaa ctcgaacagg gcgccgtgaa cctggtttgt gccgggcctg gtgaggattt
    15841 ccacctgcgc cccggcattg tggccgtact cggcggagta ggtgccggcg tgtactttga
    15901 attcctgtat ggcctcgatg gacgctctaa aaaacgtggc gttgttaatg ttccaggccg
    15961 tactgacgcc atcgtagagc acctggccct ggttctcccg catgccgttc gcactgatcg
    16021 cgatggcggt atcccggcgc ccgtactgat tggtgttgga cgaaatgcct ttgataacgc
    16081 cgggcatgag gcccgcgagg ttggcgaagt tgcgcccgtt gagcgggaga tcgaccacgc
    16141 gcctctgttc gaccacgttg cccatggtcg catcgtcggt gttcaggatg acgccgcggg
    16201 cgctcacctc taccgcctca gagaccgcgc cgacctggag ctgaaaatca acgcgggctt
    16261 tctgattgag ttgaagaatc agccccgtgc gcacatcggc cttgaaacca tcccgctccg
    16321 cacggacaat gtactcgccc ggcgccatga aagggaagac gtacgtaccg gattcgccgg
    16381 tgaccacgcg tcgaacctgt ccagtggcga cgtgggtggc gctaatggca acggcgggca
    16441 cgacggcacc gctttgatct tcaacgatgc ccaaaatctg agtggattct ccgctctgtg
    16501 cgaagccggc gggcggcaac gcaaggccga gcaacaaaac gaaacccagg tgtgtctggt
    16561 gcatttcagc ctctctgtgc ggagcattat atacccgata tacccggagc atcgccaaag
    16621 ttagcacccg taaagaacgc ttttgaaggg gagaaggtaa aaagcgggta ctaccttgac
    16681 tttgactctt tactgtgata ggttccctcc gtccggatca cgatacttgt gatcgcagta
    16741 caacgtacgg gaaagagcta gggggatttt tatatgactc gattaattcg aagaaggtgg
    16801 atttcgttcc ccttcatcgc ggtacttttc tgttgcgcgg gggcgtttgc gcaagagtac
    16861 cgcggaagag tacaaggcac ggtaaccgac ccgagccagg catccgtggc cggggcgagc
    16921 gtgacgttga ggaacgtcaa tacaggggtg gaaacttcgc gccagactga agcgaatggc
    16981 gcttacctgt tcgatttcgt gcagccgggc agttactcca ttacagtgca agccgccggc
    17041 ttccagcgtt tcgttcagga gaacattaat gtcttgacgc gcggcgacgt gacggtgaac
    17101 gccacgttaa gcgtcggcgg cgtcaccgag acggttacgg taaccgagga ggtggcccag
    17161 gtgcagttca atacctccac gatgaccacc acggtgcaag gcagcatgct gaaggacatt
    17221 cccattctgg cgcggaatcc cttcacactg gccatgctga acccggcggt aatcaacaag
    17281 tactgggacg tgtcgcaccg gaacccgttc tatatgtggt cctcgaacgg tctggacatc
    17341 ggcggacgca cgggcggcaa gaacgacatg ctgctggacg gcgtttccct gggtctgtcg
    17401 gcgcgcggtt cctacatgcc ctcgatgg
//
LOCUS       DASTMK010000010         9721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_224691, whole genome shotgun sequence.
ACCESSION   DASTMK010000010 DASTMK010000000
VERSION     DASTMK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224691"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..738
                     /locus_tag="VFQ79_00320"
     CDS             196..738
                     /locus_tag="VFQ79_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138117.1"
                     /translation="MRQHSRLAAACTAVLTALLLPQSAWANNNCHTIRGVYQSQYDAT
                     VGGWLGELSVLLDDKELLIGKIVAATPGVTIRAGAAGYDKGGYLTWAFGSGADTFRTG
                     DQHGMFIISVEGTGVGQYSGSARIVAGSGRFVNAAGSYTWSGRYLLWRSDPNDPNSAL
                     QGRWNADFNGTVCNVLPASQ"
     gene            807..2240
                     /locus_tag="VFQ79_00325"
     CDS             807..2240
                     /locus_tag="VFQ79_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0138118.1"
                     /translation="MEDERYLFRPDCVRMEGYSYAHSAPEPVRIGVLDASSEDRAGPG
                     VAGADYCRFRRGRGWCIVFLETPPTVIFFAVLACLLIPSLAAQSPKQTPAQDVPIATT
                     TTRLVEVNVIARDNKGPVAGLMVDDFILFDQGKRREIKSFSMVRSRAKSGSGAAAPEA
                     SSNALERGEDRPGNVTVILLDALNTPQRFQTFARRQVIGWLKQVEPGEEIAIYVLGRS
                     LGILHDFTSDAKSLAELLSVFRGEHVALLEASQPEPSNTGVPAVDSIIDQLNAVLAND
                     AITGRAEKTLAAVGIIAERLSPIPGRKSLVWISSGFPFSIGLHEPSLFSNPSNDNWAA
                     RRNFEKQVERVARAVNHANIAIYPVDASGLGVGASFQSADRGIGQGRPRQGSREEDAV
                     LNARATMEELAARTGGRAFYNSNDLKRAIETAIRDSELTYTLGFYPDAADMDGKFHEL
                     KVQTKRKGVRLRHRKGYLALGEGTSSN"
     gene            complement(2250..2804)
                     /locus_tag="VFQ79_00330"
     CDS             complement(2250..2804)
                     /locus_tag="VFQ79_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0138119.1"
                     /translation="MSNYKRQLFAGIRGTVLEIGAGTGANLRYMTPEQVRWTGVELNP
                     FMTSYLRREANRVNLPVSMLIAPADALPVRDSSVDAVIGTLVLCCVPSPQRCLQDVLR
                     VLKPGGKFLFIEHVAAPQGSRLRRIQNLVTPFWKRLGDGCYPNRQTSADIEAAGFESV
                     SYREITAPTPIVSPQIAGVATKAR"
     gene            complement(2896..3363)
                     /locus_tag="VFQ79_00335"
     CDS             complement(2896..3363)
                     /locus_tag="VFQ79_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138120.1"
                     /translation="MLKTCRFTSLYLTAITLSMTLCHLLEMPPKMQYGEALYMAVQHS
                     LYLHFAWVGALAELGAVGSLIALCVLVRKRGHIFHLTLAATICVAAGLAVWFVFVSPA
                     NTQMAAWTGIPLPANWTDVRRQWEFGHAASAILDLIGFGALALSVTMELRKAG"
     gene            complement(3457..4413)
                     /gene="fliM"
                     /locus_tag="VFQ79_00340"
     CDS             complement(3457..4413)
                     /gene="fliM"
                     /locus_tag="VFQ79_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEU0138121.1"
                     /translation="MDRVLSQQEIDNVFRNLRDGGKSENDALKAQSYDFRRPDRIAKD
                     QLRAIHMLHDNFARSLASSLSAYLRAYVMVNLVSVEQLSFTEFSQCLSSPTCLVSLGM
                     KPFDGKAVMEMNPSLVFPILEMLLGGSAKAESTINREITEIERSILDGLLRIILQDLK
                     QAWHAVSQIEFHVDALETEPQMLQILAPNEAVVAVSMEVRIGERSGMMNIGVPSMIVK
                     MLRQRFDQQWSVRKTESTEAEQARVLRLVKAANVRMDVRLEGPTLTVKDLMALKPGDV
                     LAFDYPVHRELDLSLNGKLKYRGRIGCSGRKRAFIVQESFSQ"
     gene            4498..5799
                     /gene="mtaB"
                     /locus_tag="VFQ79_00345"
     CDS             4498..5799
                     /gene="mtaB"
                     /locus_tag="VFQ79_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEU0138122.1"
                     /translation="MHSFHVQNFGCRATQADGAALETMLANRGMQAADGAAGADLVIL
                     NTCTVTSAADEDVRHAVRRVHRENPSARILVTGCYAQRAPEELAAIPGVWQVVGNSHK
                     TQIPDLLFEPRDDTPYHGQIHVGDIFAQQEFLAAPVEDAAGDRTRPNLKIQDGCNNRC
                     SFCIIPFVRGRSRSAPVEQVVSQVQSLAGRYREIVLSGINLGRWGREPGGRMRLAGLI
                     RLLLAETDVERLRLSSVEPMDLSDDLLDLMASSPRIAKHVHAPLQSGSDRVLRRMHRK
                     YRPHHYADRIRKARLLMPDAGIGADVMVGFPGETEEEFEENRRFIEGLPFTYLHVFTY
                     SERPGTPAALDAAQVPLPVRKQRNRILRELAAAKNLEFRRSMIGRSLPVVTLEKGNTA
                     ISGNYMKVELAYPREANRIVEVEVGGLTTGGLREKQLLPVL"
     gene            complement(5802..6401)
                     /gene="recR"
                     /locus_tag="VFQ79_00350"
     CDS             complement(5802..6401)
                     /gene="recR"
                     /locus_tag="VFQ79_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU0138123.1"
                     /translation="MPDFAEPLARLIQEFKRLPGIGQKSAQRLAFHMLRARREDADRL
                     IQAIVDVKEKLGLCAECNNISDSELCPFCRDEGRDRSTICVVEEPHNILPVETTRQYE
                     GVYYVLHGAISPLRGVGPEQLKIKGLLNKLSSGDVAEIILATNPTVEGEATASYLSRL
                     LKPLGVKVTRIAMGIPVGSDLEFADEVTMSKSLENRREM"
     gene            complement(6410..6700)
                     /locus_tag="VFQ79_00355"
     CDS             complement(6410..6700)
                     /locus_tag="VFQ79_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU0138124.1"
                     /translation="MQQMMKQAQQMQQKLQEEIAQIRVEASAGGGMVTVKMDGQKKVL
                     GVTIDPEVAGDVEMLQDMMMAAFNEAHKKVEDETQQKMGGLLGGMGLPPGLF"
     gene            complement(6750..8435)
                     /gene="dnaX"
                     /locus_tag="VFQ79_00360"
     CDS             complement(6750..8435)
                     /gene="dnaX"
                     /locus_tag="VFQ79_00360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU0138125.1"
                     /translation="MYQVIARKYRPQTFSELVGQGHVRTTLENAISQGRIAHGYIFSG
                     QRGTGKTTVARILARCLNCVNGPTAQPCGVCSSCTEIAAGNAVDVIEIDAASNRGINE
                     MRELRENVRYRPARDRYKVFIVDEAHQITHEAFNALLKTLEEPPEWVVFILCTTEAHK
                     IPTTIASRCQQFSFRSVDFEELIARMAWICAQEGVEADPETLAVLAQAGEGSVRDSLS
                     ALDQAIACCGSTLNADEVRTLLGMFSLDSLHQVTQALSGGTSQQMLEIVDDLERNGRH
                     LQHFCRELARYFRSLLVVRVAGKGTRLVAATGGEQEKLADIAQSFSEEDLTRYLQLTL
                     DLFRDLQFSLQPRLHLEIGLLKLLHAGKLAPIEEALAMLKEPGSSQSRPSTPTGPPRV
                     QGSAAPAPPSVGPSPFAMDTRRKTQTAPPTEAAAAVAPPSPAAGGTLKSRLHAWLVER
                     EMLAIADAVEHSQVAESAAEIVFRAPREFWLALRSGDMQQAVRAVLGRVLKVKVENGE
                     SAAQPLAMEQNPAPQTTGEAGDASERALSHPDVQRFREAFPESQIRTVRNLKE"
     gene            complement(8485..8582)
                     /gene="ffs"
                     /locus_tag="VFQ79_00365"
     ncRNA           complement(8485..8582)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFQ79_00365"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8792..9211
                     /locus_tag="VFQ79_00370"
     CDS             8792..9211
                     /locus_tag="VFQ79_00370"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0138126.1"
                     /translation="MENSEHQLLTPSDGIPAAAPVPPSFRLFNERRTCVRYPIALDLR
                     YRASTPEVRGLGQQSTTVEISSQAISFSTPEPLPAGAHVETSIRWPFLLDNRTPLQLV
                     ADGKVVRSTEGKTVIRIHKYEFRTCRKQAFDATSGLT"
     gene            9275..9679
                     /locus_tag="VFQ79_00375"
     CDS             9275..9679
                     /locus_tag="VFQ79_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0138127.1"
                     /translation="MSSSHNDTPTTILVIDPDLGFILVLSRALLSAGYQVIPASTMEE
                     AARLTSELELGIDAVVAEAGALASGGSDPMGGLGRWAARSAMIVATSTPEALAGKLPA
                     RPAAVLRKPQANEPDLTEEWLRIIRQVLALSR"
BASE COUNT         1970 a         2942 c         2916 g         1893 t
ORIGIN      
        1 acagaacggt gctcggcggc aagggttcgc tacgccgcgg caaaccccgc cgcgcccttg
       61 cctcctgcgc gccgttctgc aagagcgagc gattgcgacg ggcggctccg acggaaacca
      121 gaagcagcgc agtttcttcg ccttacgaaa gacgaaaacc aggttgtagt tgacaaaaca
      181 tcataggaga aatccatgcg acaacactca cggcttgcag ccgcctgcac cgcggttttg
      241 acagctttac tgctccccca gtccgcgtgg gcgaacaata attgccacac gatccgggga
      301 gtctatcaat cccagtacga cgccaccgtc ggcggctggt tgggcgaact ctccgtcctt
      361 ctggacgaca aggaactgct gattggcaag atcgttgccg ccactcccgg cgtcacaatc
      421 cgcgccggcg ccgccgggta cgacaaagga gggtacctga cgtgggcgtt tggatctggg
      481 gcagacacgt tcaggacagg cgaccaacat ggaatgttca tcatctccgt tgaaggtacc
      541 ggcgtgggtc aatactccgg ttccgcaaga atcgtggctg gcagcggccg gttcgtgaac
      601 gcagccggca gctatacatg gtcggggcgc tatcttctct ggaggtctga tccgaatgac
      661 ccgaattcgg cgttacaagg gcgatggaat gccgatttta acgggacggt ctgcaatgtg
      721 cttccagcaa gccagtgaga acgctactca aaacttgagt aggactgccc aaaagtgagg
      781 cacccgaaat gtcacacttc cattgaatgg aggacgaacg ctatctgttc cgtccggact
      841 gtgtacggat ggagggatat agctacgctc attccgcgcc agagcctgtg cgcattggag
      901 tattggacgc gtcgtccgaa gaccgcgcgg gccctggcgt tgcgggcgcg gattattgcc
      961 gtttccgccg cggccggggc tggtgtatcg tattccttga gacgccgcca actgtgattt
     1021 tctttgccgt tctcgcgtgc cttctgattc cttctctagc cgctcagtcc ccaaaacaaa
     1081 ctccggcaca ggatgtgccg atcgcaacca ctaccacccg tctggtggaa gtgaatgtga
     1141 tcgcgcgcga caacaagggc ccggttgccg gcctcatggt agacgatttc atcctgttcg
     1201 accagggaaa acggcgtgag atcaaatcct tttcgatggt gcggtccaga gcgaaatcag
     1261 ggtctggcgc tgcggcgccg gaggcttcct cgaacgcctt ggagcgcggg gaagaccgcc
     1321 ccggaaacgt cacggtgatt ctcctggatg cgctgaatac gccgcagcgg tttcaaacgt
     1381 tcgcacgccg gcaggtcatc ggctggctga agcaggtgga acccggagag gagattgcca
     1441 tctacgttct gggcagatcc ctcggcatcc tgcacgattt caccagcgac gcgaaaagcc
     1501 tggcggaact gctctctgtt tttcgggggg agcatgtggc cctactcgaa gcatcgcaac
     1561 cggagccttc caacacgggc gtgccggcgg tcgacagcat catcgatcaa ttgaatgcag
     1621 tgctggcgaa cgacgcaata acgggcaggg cggaaaagac tctggctgcc gtcgggatca
     1681 ttgccgagcg gctgagcccg attccaggcc gcaagagcct ggtttggatc tcgagcggtt
     1741 ttcctttctc catcgggctg cacgaaccca gtctgttcag caatccttcg aacgataatt
     1801 gggccgcgcg gcgcaacttc gaaaagcaag tcgagcgtgt ggcgcgcgct gtgaatcacg
     1861 ccaatatcgc catttacccg gtggacgcga gcggcctggg agtcggcgcg agctttcagt
     1921 ccgccgacag aggcatcgga caaggaaggc cgcgccaggg atcacgggag gaagacgccg
     1981 tgctgaatgc acgcgccacg atggaagagc tcgcggcgcg gaccggcggg cgcgcgtttt
     2041 acaattcaaa tgatctgaag cgcgcgattg aaacggccat ccgggattcc gagctcactt
     2101 acacgttggg cttctatccc gatgcggccg atatggatgg aaagttccac gaactgaaag
     2161 tacagaccaa acgcaaaggc gtccggctgc gccaccgcaa aggatatctg gcgctcggag
     2221 agggaacgtc ttcgaattaa ggaaggtggt cagcgggctt ttgtcgccac gccggcaatc
     2281 tgcgggctca cgatcggggt gggagccgtg atctcgcgat aagaaacact ctcgaagcca
     2341 gcggcttcaa tatcggctga ggtttggcga ttggggtagc atccatcgcc tagtcgcttc
     2401 caaaatggag tcaccaggtt ctggatgcgc cgcaggcggc ttccctgtgg tgccgctacg
     2461 tgctcgatga acagaaattt tcctccgggt ttcaggacgc gcagcacatc ctgaaggcag
     2521 cgttgcggac tgggaacgca gcagaggaca agcgtgccga tcacggcgtc gacactgctg
     2581 tcgcgcacag gcagcgcgtc cgccggagcg atcagcatcg agaccggcag attgacgcgg
     2641 ttcgcctccc gccgaagata ggacgtcatg aacggattga gctcgacgcc ggtccagcgc
     2701 acctgttccg gcgtcatgta acgaagattc gcccctgtcc ccgctccgat ttcgagcacg
     2761 gttccgcgga tcccggcaaa gagttgccgc ttgtagttcg acacaaactg ctcgtagcgc
     2821 ttgttgaagc gggccagtgc ccacgtgaag atccgctgtc agaccagcct taaaagcatc
     2881 catacactac ccggctcaac ccgccttccg gagctccatc gttaccgaca gcgcgagagc
     2941 gccaaacccg atcaggtcga ggatggcgga cgcagcgtgc ccgaattccc attgcctgcg
     3001 tacgtccgtc caattcgcgg gcaggggaat accggtccac gccgccatct gcgtattggc
     3061 gggggacacg aacacgaacc agacagcaag cccggcggcc acgcaaatgg ttgcggccag
     3121 agtgagatga aaaatgtgcc cgcgcttccg gaccagcacg caaagtgcga tcagggagcc
     3181 caccgcgccc aactcggcca gagcccctac ccaggcgaag tgcaggtaga gcgagtgctg
     3241 caccgccata tataacgctt cgccatattg catcttggga ggcatctcca acaagtggca
     3301 aagagtcatc gaaagggtga tcgcagtgag gtagagactg gtgaaccggc aggttttcag
     3361 caagatcgca aactccttac ggtcatgcct ggcgcctgtt ccgccgagcc cgccattact
     3421 actgatttca gtctacactg cgcgatgcgc gcggattcac tgactgaagc tttcctgcac
     3481 aatgaaagcc cgcttgcggc cggaacaccc tatgcgtcca cgatatttga gcttgccgtt
     3541 caggcttaga tccaactcgc ggtgcaccgg gtagtcgaac gcaagaacat ccccgggttt
     3601 cagagccatc aggtctttaa cggttagcgt ggggccttcg agccggacat ccatgcgaac
     3661 gttcgccgct ttcacaagac gcaggacacg agcctgttcg gcttccgtcg attccgtttt
     3721 ccggaccgac cattgctgat cgaagcgttg ccggagcatc ttgacgatca tcgacggcac
     3781 gccgatgttc atcattcccg atcgttcgcc aatgcgaact tccatgctta cggcgaccac
     3841 ggcttcattc ggcgccagaa tttgcagcat ctgcggctcg gtctcgaggg cgtcgacatg
     3901 gaactctatt tgggaaaccg catgccaggc ctgtttcagg tcttgcagga tgatcctgag
     3961 cagcccatcg agaatgcttc gctcgatttc cgtgatctca cggtttatgg tgctttccgc
     4021 ttttgcgctg ccgccgagaa gcatttcaag aatgggaaat accagcgacg gattcatttc
     4081 catgactgct ttgccgtcga agggcttcat cccaagagag acaaggcagg ttggagacga
     4141 caggcactgc gagaattccg tgaacgacaa ctgctctacc gagaccaggt tgaccatcac
     4201 atacgcgcgc agataggccg aaaggctgga ggccagagaa cgcgcaaagt tgtcgtgcag
     4261 catgtggatg gcccgcagtt ggtctttcgc gatccggtcg ggccggcgga agtcgtaaga
     4321 ctgggccttc agggcgtcgt tctccgattt tccaccgtcc cgcaggttgc ggaacacatt
     4381 gtcgatttcc tgctgcgaaa gaacccggtc caaggtgcgg cctccgttca acaagcctgt
     4441 acacttatcg gccggcaggg gggaaacttt agcgtgcaag gtagaatagg cagagaagtg
     4501 cacagctttc acgtacaaaa tttcggctgc cgcgccacgc aagccgatgg agcggcgctc
     4561 gaaaccatgc tggcgaatag gggtatgcag gccgcggatg gcgcggccgg cgccgacctg
     4621 gtgatcctca acacctgtac ggtcacctcc gccgccgacg aggatgttcg gcacgcggtg
     4681 cggcgcgttc atcgggaaaa cccttccgcc cgtattctgg ttacgggctg ttatgcgcag
     4741 agggcgcccg aagaactggc ggcgattcct ggtgtgtggc aggtggtggg aaacagccat
     4801 aagacgcaaa ttccagacct gcttttcgaa ccgcgagacg acaccccgta ccacggccaa
     4861 atccatgtgg gagacatttt tgcccaacag gaattcctcg ccgccccggt ggaggatgcc
     4921 gccggcgacc gcacacgtcc gaatttgaag atccaggacg gctgcaataa ccggtgttcg
     4981 ttctgcatca ttccgttcgt gcgcgggcgc agccgcagcg cgcccgttga acaggtggta
     5041 tcgcaggtgc aatcgctggc cggccgttac cgcgagatcg tattgagcgg catcaacctg
     5101 ggacgttggg gacgcgagcc gggcggccgc atgcggctgg ccggccttat ccggctactg
     5161 ctcgcggaga cggacgtcga gcggctgcgg ctgagctcgg tggaaccgat ggatctctcc
     5221 gacgatctcc tcgacctgat ggcgtcgtcg cctcgcatcg caaaacacgt gcatgccccg
     5281 ctgcaatccg ggtcggatcg cgtgctccgg cgcatgcatc gcaagtaccg gccgcaccac
     5341 tacgcggatc gcattcgaaa agcccggctg ctgatgccgg acgccggtat cggcgcggac
     5401 gtaatggttg gctttccggg cgagacagag gaagagtttg aagaaaaccg ccggtttatc
     5461 gaaggtcttc cgttcaccta tctccacgta ttcacctatt ccgagcggcc cggcactccg
     5521 gcggcgctcg atgcggccca ggtgccccta ccggttcgca agcagcgcaa ccgtattctg
     5581 cgcgaactgg ccgcggccaa aaacctggag ttccgtcgaa gtatgatcgg ccgttcgctc
     5641 ccggtcgtga cgctcgaaaa gggaaatacg gcgatcagcg gcaactatat gaaagtggaa
     5701 cttgcctacc cgcgcgaggc gaaccgcatc gttgaagtgg aagtaggcgg cctgaccacc
     5761 ggcggtttgc gcgaaaagca gctcctgccg gtgctgtagt cctacatctc ccgccggttc
     5821 tcgagagatt tcgacatcgt tacttcgtcg gcgaactcca gatcgctgcc aaccggaatc
     5881 cccatcgcga ttcgcgtgac cttcacaccg agaggcttca atagccgcga gagataagag
     5941 gcggttgcct cgccttccac ggtagggttg gtggcaagaa tgatttccgc cacgtcgccg
     6001 gaggaaagct tgttcaggag ccccttgatt ttcaactgtt ccgggcccac accgcggagc
     6061 ggcgaaatcg caccgtggag aacatagtag accccttcat actggcgcgt ggtctcaacc
     6121 ggcaggatgt tgtgcggctc ttcgacgacg caaatcgtgc tgcggtcgcg gccttcatcg
     6181 cggcagaagg gacacagttc gctgtcgctg atgttattgc actccgcgca gaggccaagc
     6241 ttttccttta cgtcgacaat cgcctgaatc agccggtctg cgtcttcgcg ccgggcgcgg
     6301 agcatatgaa acgcaagacg ttgcgccgac ttctggccga ttcccggcag gcgcttgaac
     6361 tcctgaatca atcgggccaa cggttccgca aaatcgggca tattggcggc tagaacagac
     6421 ccggaggcag acccatgcct cccaggagtc cacccatctt ctgctgcgtt tcgtcttcca
     6481 cttttttatg agcttcgttg aaagccgcca tcatcatgtc ctgcagcatc tcgacgtcgc
     6541 cggccacttc ggggtcgatg gtcacgccca gcactttctt ctgcccgtcc atcttcacgg
     6601 ttaccatgcc gcctccggcg gaggcctcga cgcggatctg tgcaatttcc tcttgcagct
     6661 tctgttgcat ctgctgggcc tgcttcatca tctgctgcat gttaccgggt agtttcacgt
     6721 tgtcgctcct tatttgatac cggccaaaac tattctttca gatttctgac ggtgcgaatc
     6781 tggctctccg gaaacgcctc gcgaaaacgt tgaacatcgg gatgagacaa tgcccgctcg
     6841 gatgcgtccc cagcctcgcc cgtcgtctgg ggcgccggat tctgctccat cgccaacggc
     6901 tgcgcggccg attcaccgtt ctccactttc acctttaaga cacgtccgag aaccgcacga
     6961 accgcctgct gcatgtcccc ggaccggagt gcgagccaga actcccgcgg cgcccgaaac
     7021 acaatctccg cggcggattc ggcgacctgg gagtgctcca cggcatccgc gatagccagc
     7081 atctcacgct caacaagcca ggcatgcagg cgggatttca acgttccccc agcggcgggt
     7141 gaaggagggg cgacagccgc cgcggcttcc gtgggcggcg ccgtttgcgt tttccgccgg
     7201 gtatccatag cgaaagggga cgggccaacg ctgggcggcg cgggggcggc tgatccttgc
     7261 acgcgcggcg gtccggtggg tgtggacggg cgggactggg acgaaccagg ctccttcagc
     7321 atcgccagcg cttcttcaat gggcgccagt tttcccgcgt gtaacagctt gagaagaccg
     7381 atctctagat gcaggcgcgg ctggagagag aactgcagat cccggaacag gtccagagtg
     7441 agctggagat accgggtgag gtcttcctcg gaaaacgact gggcaatgtc cgccagcttc
     7501 tcctgttcgc cgccggtcgc cgccacaagc cgtgtgcctt ttcccgccac tcgtaccacc
     7561 agcaggctcc ggaaatatcg cgcgagctcg cggcagaagt gttgaagatg ccgcccgttg
     7621 cgctccagat cgtccacgat ctcgagcatc tgctgcgaag tgccgccgga gagagcctgc
     7681 gttacctggt gcagcgagtc gagagagaac atccccagca gggtcctcac ttcgtccgcg
     7741 ttcagcgtgg agccgcagca ggcgatcgcc tggtccagag cggagaggga atcccggaca
     7801 ctgccttcgc ccgcctgggc cagtacggcc agcgtttccg gatctgcctc tacgccttcc
     7861 tgcgcgcaaa tccatgccat gcgcgcgatc agttcttcaa aatcgaccga ccggaagctg
     7921 aactgctggc agcgcgaggc gatggtagtg ggaatcttgt gcgcttcggt ggtgcaaagg
     7981 ataaatacca cccattccgg gggctcttcg agcgtcttca acagcgcgtt gaaagcctcg
     8041 tgcgtgatct ggtgcgcctc gtcgacgatg aaaaccttgt agcggtcgcg agccggacgg
     8101 tagcgcacgt tctcgcgcaa ttcccgcatc tcattgatcc cgcggttgga ggcggcgtca
     8161 atttcaatga cgtccacggc gtttcccgcg gcgatctccg tgcaactgga gcagacgccg
     8221 cacggctgcg cggtggggcc gttcacgcag ttcaggcatc gcgccaggat tcgcgcgacc
     8281 gtggtcttcc ccgtgccgcg ctgccccgag aagatatagc cgtgtgcgat gcggccttgc
     8341 gaaatagcgt tctccaaagt ggtgcggaca tggccctgtc ctaccagttc ggaaaacgtc
     8401 tggggcctgt attttctggc aataacctga tacatgagag tctacagcga gggcgcgccg
     8461 gccggccgcg agtgcgaaac tgccagaccc cgggtgatcc gcggcacacg agctaaaccg
     8521 ctaccgttgc ttccttccgg acctggcggg gtttgcagcc gcacgttgca cggagcccga
     8581 ggcctgacaa actagaattc tagcatgtgc ccgcgctttc gaacacatca gggagcggga
     8641 accgaaaaaa ggcggaagtc aatgaccaag gttctgtgag tactgttatt tgagaagcct
     8701 cccgccagga cggatatggt attgacacta cccgttcgaa ctgcgcataa tcgttcaccg
     8761 gtaaactccg aagaaggggc ataagtcacc tatggaaaat agtgaacacc agttgttgac
     8821 cccgtctgac ggcattcccg ctgccgcgcc ggttccccca tcgttccggc tgttcaacga
     8881 gcgccgcact tgtgttcgtt atcccatcgc gctcgatctt cgataccgcg cttccacgcc
     8941 ggaagtaagg ggcctcggac aacaatccac tacggtagag atcagcagcc aggcgatctc
     9001 gttctccaca cctgagccgc tcccggcggg cgcgcacgtg gagacgtcga tccgctggcc
     9061 gtttctgctg gacaaccgga cgcctctgca actggtggcg gatggcaagg tggtgcggag
     9121 cacggaggga aagacggtga tccgaattca caagtatgaa ttccgcactt gcagaaagca
     9181 ggcattcgac gcaacttccg gcctgacctg agtactgccg aaaaaccgat gttgccgccg
     9241 gtaaaggcag aaaagggctg agatccaatc tcccatgtct tcgtcgcaca acgacacgcc
     9301 gacgacaatt ctcgtcatcg atcccgatct tggattcatt ctcgttttaa gcagggcgtt
     9361 gctctccgcg ggctatcagg ttatccccgc gagcacgatg gaggaagcgg cgaggctcac
     9421 aagtgagttg gagctcggaa tcgatgccgt tgtggcggaa gccggcgccc tcgcctctgg
     9481 cggatcggac ccgatgggag ggttagggcg gtgggcggca cggtccgcga tgatcgttgc
     9541 cacgtcaact cccgaagcgc tggccggcaa actccccgcc cggccggcag cggtacttcg
     9601 caaacctcag gccaacgagc cagacctgac agaggagtgg ctcagaatca tacggcaggt
     9661 cctcgctctc tcccgttgaa aatccggcct tgacagccgg cagtaacccg gctacacctt
     9721 c
//
LOCUS       DASTMK010000011         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_128889, whole genome shotgun sequence.
ACCESSION   DASTMK010000011 DASTMK010000000
VERSION     DASTMK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128889"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1139
                     /locus_tag="VFQ79_00380"
     CDS             207..1139
                     /locus_tag="VFQ79_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU0138128.1"
                     /translation="MLLPFRLVYHEGYDLNLGDHVFPSQKFKLVRSRLLREGFAERDD
                     FMEPEPASDEDILLVHTPEWIHKLKTGTLTYRDIVTLEIPYSQQMVRAFWLAAGGGIL
                     AARLALEHGIGFNIGGGFHHAFPGHGEGFCAINDIAIAIRRLLADGLIHKAMVVDCDV
                     HQGNGTAAIFAGDESVFTISIHQFNNYPSVKPPSTVDIHLPDGVSDQEYLNRLRDPYS
                     TALTGFLPELLIYVAGADPYFGDQLGGLSLTLEGLKERDRMVMETALEYRVPVVVVFA
                     GGYALQLADTIAIHTNTTLAAAEVLKDTGWHGRR"
     gene            1413..>3094
                     /locus_tag="VFQ79_00385"
     CDS             1413..>3094
                     /locus_tag="VFQ79_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138129.1"
                     /translation="MPSTSQVIFVTSDDLVSEQSRTREDPALTIAHSVTLTELRPGQS
                     YTIYAASRGADNAAASSKASPTTFVTQNIDPNANLDFRLRLSGPKRVYAGSDLYLVLS
                     STQLAGPAKHLYLLGASGLPASISYRPVCRMQNPTTDEKNDCLVDSATSTPFVWYGSD
                     NAKERTLVRLRTRADTAPGPYTVEVTARAGSVTRTAGYSFTVEPAPVRPQSVRPSAVP
                     AIPGLAKWESSMKTLGKKWCIPGQVMSFGYEGEVWYYDGGRVYLQIADYTGDASWLSC
                     AQNIIKQYRDQAIARNGNVQQWRVFPHGLAMNYWRTNDEASRNAVILLSKNAPYATKS
                     GSPFVSAIRETSYVIETYVKAEQLTGQRDPHLSRAVDFLIGVFDQLFTPDANVLHQLF
                     FDGLGAEALIQYYELTEDPRIPPTIKRMLDWIWTHGRDSSGNKLLYNPLAVPLSSSTT
                     YTNLVVPAFAWYWSVTGDEVYRQRGDHLFLHALDVDISYSGKIFSQNYRWSFDFVRWR
                     SGGKYSATHPLANRPGTTFVLRPEITSSNSKVAWTTLVPSSSQVEFGPVGAEP"
BASE COUNT          659 a          878 c          871 g          686 t
ORIGIN      
        1 aggaaggatg ccgccagcag caggaccgta gacaccgcga caacccggcg gtatggccgc
       61 atgtaggcga gcagccgccg catgagacgg ccatcatatg ctttttctaa gacctcttct
      121 tccacgcgct cttcttatta catcatggcc gcgcgccgtt ctccggacgg atccgatgtt
      181 atgctgcgga cgtaggcccg aacacgatgc tcctgccatt ccggcttgtt tatcacgaag
      241 gatacgacct caatctcggc gatcacgttt ttccctccca gaagttcaaa ttggtgcgct
      301 ccagattact gcgcgagggc ttcgcggaac gggacgactt catggagccc gagccggcgt
      361 cggacgaaga tattctactg gtgcacacgc cggaatggat tcacaaattg aaaacgggca
      421 cgctgactta tcgcgacatc gtcacgctgg agatccctta ttcgcagcag atggtgcgcg
      481 cgttctggct ggcggccggt ggaggaatcc tcgcagcgcg gctggccttg gagcatggaa
      541 tcggtttcaa catcggcggc ggtttccacc acgcgtttcc cggacatggc gagggcttct
      601 gtgcgatcaa cgacattgcc attgcaatcc ggcggcttct ggcggacgga ctgatccaca
      661 aagcaatggt tgtcgattgc gatgttcacc agggcaacgg caccgcggcc atcttcgccg
      721 gcgacgagtc cgtgttcacc atttctatcc accaattcaa taactatccg agcgtgaagc
      781 cgccttcaac cgtcgatatt catctccccg acggcgtctc ggaccaggaa tatctgaacc
      841 ggctgcggga tccctattca accgcgctga ccggatttct tccggagtta ttgatctatg
      901 tcgccggtgc cgatccttat tttggcgacc aactcggcgg actaagtctc acgctggaag
      961 ggctgaagga gcgggatcgt atggtgatgg aaacagcgct cgagtaccgt gtgcctgttg
     1021 ttgtggtctt cgcgggaggg tacgcgctcc agttggccga tacaatcgcg attcacacga
     1081 ataccacgct ggccgccgcg gaggtcctga aagacaccgg ctggcatggc cgccgttagc
     1141 ggcgttcgtt tgcccccgca gcttcccgag cccgccggca ggcatttcct tcacagaact
     1201 cagtaaatcg acccacgtag ccgcctaagg gtttcgccct gccgagccga tagaagagca
     1261 tgggtggaaa ttcatactcg tccgctacgt gcatttggct gttgagcctc gcactaatag
     1321 cctggcctgc gtcggcaggt ccaccagtaa tctctgcggt tcaggtgaca gggatcacca
     1381 tagactcggc aacagtcacg tggataacca gcgtgccttc aacatctcag gtgatctttg
     1441 taacttcgga tgacctcgtt tcggagcaat cccggacaag ggaagatccg gcattgacca
     1501 tcgcgcattc tgtaacactc acagagctgc ggccaggcca gtcgtacaca atctacgcgg
     1561 catcccgcgg cgcagacaac gcggcggcaa gcagcaaagc tagcccaacg acattcgtca
     1621 ctcagaacat tgaccccaat gcgaatctcg atttccgctt gcggctgagc ggccccaaaa
     1681 gagtctatgc cggttcggat ctttacctgg tcctgtcgag tacacaactc gcgggccctg
     1741 ctaagcactt gtacctgctc ggggcaagcg gcctgccggc ctctatttct tatcggccgg
     1801 tttgccgtat gcagaacccg actaccgacg aaaaaaacga ctgcctggta gattccgcta
     1861 cctctacgcc gtttgtctgg tatggttccg acaacgccaa ggagagaacg ctggtccggc
     1921 tccggacgag agcggataca gctccgggac cgtacaccgt ggaggtgacg gctcgtgccg
     1981 gcagtgtcac gcgaacggcc ggctacagct tcaccgtgga acctgcgcct gtcaggccgc
     2041 aaagtgtccg gccaagcgcc gtgccggcga ttcccggcct ggcgaagtgg gaaagcagca
     2101 tgaagacgct gggaaagaag tggtgcattc ccggacaagt gatgtcgttc gggtatgagg
     2161 gtgaggtttg gtactacgat ggaggccgcg tctacttgca gatcgccgat tacaccggtg
     2221 atgcatcctg gctgagctgc gcgcagaaca tcataaagca atatcgcgac caggccattg
     2281 cgagaaacgg aaatgttcag caatggcgcg tgtttccgca cggcttggcg atgaattact
     2341 ggcgtacgaa cgacgaggcc agccgaaatg ccgttatttt gctctcgaag aacgccccgt
     2401 atgccaccaa gagtggatct ccttttgtaa gtgccattcg agaaacatcc tatgttatcg
     2461 aaacgtatgt caaggcggaa caattgaccg ggcagcggga tccgcatctg tccagggcgg
     2521 tggactttct tattggtgtg ttcgaccaat tatttactcc ggacgcgaac gttcttcatc
     2581 agcttttttt cgatggctta ggcgcggagg ctcttatcca gtattatgaa cttactgaag
     2641 atcctcgtat tccgccgacc atcaagagaa tgctcgattg gatctggacg catggccgcg
     2701 actccagcgg gaacaagttg ctttataatc ctctcgcggt gccgttaagc agttcaacga
     2761 cgtataccaa tctggttgtg cccgccttcg cctggtactg gtccgtgaca ggcgatgaag
     2821 tttaccggca gcggggtgac catttatttc tgcacgcttt agatgtagat ataagttaca
     2881 gcggtaagat cttcagtcag aattaccgat ggagcttcga ttttgtaaga tggcgctcgg
     2941 gagggaagta ttcggctacg catccccttg ccaaccgccc cggaacgact tttgttctaa
     3001 gaccggaaat cacatcttct aattcgaaag tcgcctggac aaccttggtt ccttcgtcgt
     3061 cgcaggtgga atttggacct gtgggggcgg aacc
//
LOCUS       DASTMK010000012        14093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_192851, whole genome shotgun sequence.
ACCESSION   DASTMK010000012 DASTMK010000000
VERSION     DASTMK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14093
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192851"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFQ79_00390"
     CDS             complement(<1..649)
                     /locus_tag="VFQ79_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138130.1"
                     /translation="MAPVPIQQQQALVERLYTACGGSYSPEIRRKYFISGPQWAAAYQ
                     GQALFHAPTRQEVGFEQIIRKWGEIGIGHWTTHDTDVIPTSAIGTDKQWEIVASINKA
                     LQENDVKCSMVTTETFHHAVWAASPAAEAPEVREYAKFRVINTVKIGHELGAQFAVYW
                     PGSLGYYVQGAIEETQTLRWYADALNAACDADVEIAKKRGRPVLKHCLEAKPFEPQ"
     gene            755..2686
                     /gene="acs"
                     /locus_tag="VFQ79_00395"
     CDS             755..2686
                     /gene="acs"
                     /locus_tag="VFQ79_00395"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU0138131.1"
                     /translation="MTETSTYAPNPEFVAQANVSGMEAYRELYRRAEETPEEFWAEVA
                     ERELDWFEKWSHAFEWNPPFAKWFVGAKTNVAYNCLDRHLKNGRAQKIAILWEGEPGD
                     ERKITYQELHSMVCRFANVLKNRGYKSGDRAIIYMPMIPELPVAMLACARLGITHSVV
                     FGGFSAEALKARIKDLGATVVITADGGWRRAKEVKLKAAVDEALLECPDVRDVIVWKR
                     TGSDIPMKDGRDHWWHDLEEGVSSECPAEPLDAEHPLYVLYTSGTTGQPKGILHTTGG
                     YMVQTTATMRWIFDLKENDVYWCSADIGWVTGHSYIVYGPLAAGATTVIYEGAPDTPA
                     FDRWWSIVERHRITILYTSPTAIRALIRQGDDWPNKHDLSSLRLLGSVGEPINPAAWE
                     WYHRVIGKQRCPIVDTWWQTETGSIMIAPMPGAIPAKPGSGTLPLPGVLPQVVDMSGK
                     PVGVDQEGFLVIRRPWPAMIRTIWGDPKRYESQYWGRVPGVYFTGDAARCDADGYYWI
                     LGRVDDVMNVSGHRLSTMEVESALVRHPAVAEAAVVGKPHEITGQAVCCFVTLKQGNH
                     NHNELGAELRKWVAHEIGAFARPEEIRFTEALPKTRSGKIMRRLLREIVTTRTVTGDV
                     TTLEDLTVVTRLAAQRDEE"
     gene            2727..3587
                     /locus_tag="VFQ79_00400"
     CDS             2727..3587
                     /locus_tag="VFQ79_00400"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEU0138132.1"
                     /translation="MSQVAALHSDYVGLALGSLKRLPPFSPVLNRLLASLANEEVSFG
                     RMAELIEKDTVLTGHVLSMVNSAAYARAAAIVSVRHAVSLLGVLKLRNLVLGLSISRL
                     WANVQTPPGWSHARFNLHSITAATLADLLVQKVPVAFPEGAFIAGLLHDFGKLLIATA
                     LPREYGQIQQLRRSGAAEIECEMEVLGITHAELSQLALRAWNVPPAIQDAVRRHHDLR
                     PIGSSLDLAAVIGCADAYLNATGMGMSSSTPSEPETAVALLEPLEINDSLPAVLDLFT
                     TEVEAIRSFF"
     gene            complement(3584..4726)
                     /locus_tag="VFQ79_00405"
     CDS             complement(3584..4726)
                     /locus_tag="VFQ79_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEU0138133.1"
                     /translation="MLPKSEREPVSYGKGSLHPSEHSSYRDRVTGALVHQLTSHPSIN
                     HPTYFLQSSFTPDGRHILFTSYRTGSAQLFEAAFPDGEIRQLTAGLPIHAFSPAIHPD
                     GATVFFVRGGSVWSIDRKTLEERCIVTFEEAQLGECSLSRDGGWLTAAIKQLGQNGVV
                     VGRTDGTGWNLIPFPRTVIHPQFHPLDSEWLEFAGDPAPRMHRVRRDGSSLECLYQHG
                     NDEFVVHETFLGNTGNIVFTVWPYALKRLNWDTREVTVIAEFNAWHITPDRAGSRVLC
                     DTNHPDDGLFLVDVATGERNRVCLSEASSQGSQWKTSRYALAEDFAAARSVLGSGTLS
                     WMELSTDTVYGPQWTHPHPSFSHDEKLVAFASDKTGHTQVYAAELN"
     gene            complement(4710..5516)
                     /locus_tag="VFQ79_00410"
     CDS             complement(4710..5516)
                     /locus_tag="VFQ79_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138134.1"
                     /translation="MMLRFVLAGACVFTGLCFGQAAEDLFSKAPPDVDAALRGRIAKF
                     YQAHVDGKFRVADEVVAEDSKEVFFAAQKTRYKGFEIVKIAYSDQFTKARVVVAVDTD
                     MAFPGMGKVAVKAPLTTLWKLENREWFWYVDPNLGKETPFGRMKPGEGESSGGLFLNK
                     GPDPQALIKMVSVDKTKVQLSGESPTAEIHVSNGMSGAIALSLEYSPAAGFEASLDTK
                     EVARGGSAKILIRSVPGEKAPGSSITIRLHVQPLGHTIPIEVQFANAAKK"
     gene            5711..7900
                     /locus_tag="VFQ79_00415"
     CDS             5711..7900
                     /locus_tag="VFQ79_00415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0138135.1"
                     /translation="MTTSAVTPKKAALRTLPGDDVRQIMWRFAGRYELQMLVQASRSA
                     ARGPVARLVAEGARNTHEWTREKAQLLTAFDEAGITSVFMDPEQGGFIEGPKNLALAL
                     VAFELAWVDAGAATGSLAGCLALAPIHERGTPAQRDEYMKRCTPPAPGEDRKPWRGAF
                     ALTEPIPYVGVETGLLSGKVRVVEWPDGGEPILQVDKRGRFITNMGFANFVTAAVDSG
                     DERIKGSSMIILEETDPGIFDRGIPTRKLVHQLSSTRDPVLSLRVPASRIIGGYTIKD
                     GVIVPRYSHGEIIEAVFRRTRVTVGLMTSAKLLSAVEPVLRYQRQRFRGADAVAPGSP
                     RYELGLQQREDSLHRLIDVWATGEASASLGFAAARLFDELDPLEREKDRLLEEQGILG
                     GSAQFKALRKRQNEALQAISSRVMPEDPLLRFLVLDSVANVLCPATKLWNTGHGANMM
                     REAVSLMGGYGITEDCPGFLGHKWMDAQLEATYEGPEAVQRRQLAVTMTNELFLKQYR
                     QWIAAMREIASSKPGTGACTLATAMQMWLWTLERLQKSSDAEGSKLYQNARQGVTFAL
                     ADALCWVLAARQMILDLLELEAKGPENPTVAEALPGLAAFYTDLAHVQSARSAGEVGR
                     ICAELVFGYNRHPAWDEQGCGSCYSSDDLTALENLIPGIASCAVDVVESDGSHPMKAG
                     PCVRFDGMEQFTRLRTRLDGCLTGARLAKDRAAEALTRVMIPEALDY"
     gene            7905..9752
                     /locus_tag="VFQ79_00420"
     CDS             7905..9752
                     /locus_tag="VFQ79_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU0138136.1"
                     /translation="MNVERQSMDVDIVCVGFGPAAGGFLTTLSRGILNPDGTPAVESS
                     AMPGMPPQVVCYERADDIGFGVSGAVTRARGIRASFPDLDLSQIPMSARVEKEKVLYL
                     LDPVGASRRSRALRAADALIRGFRFALPLECDALELPYTPPFLRKDGGLLLSIGQFNQ
                     WVGQSLMAEGAVQIWPGSPVSQALVENGQVTGVRLLDQGTDKSGNPEAGFLPGMDVRA
                     ALTVIADGPVGAVGRQMDEQFGMPDGHSRQDWAVGAKMVIELPEDTPLETGTVLHTFG
                     YPEPEIFGFLYVYADRLASAGIFVPSWFDNPVRTSYRYLQHFLLHPYLWRYVKGGRLL
                     SWGAKTLQESGRRGEPYLAGDGYARIGEGSGSTNVLTGSGVDEAWTTGVLLGESVLEL
                     LKQGRPFTRDNLDSTYVAKRRQSWVEREGRIAEKARDGFHNGVVTGLIGMALTGLTGG
                     HLAMPGKPRPVYEDLPDVYDYYRKKIGAEEMARIRRECTEKRLPLHDTLMERCGWPKI
                     PYDGKLLISHQDALLVGGKVQAAPGYADHVVFLYPSLCEACGTQICVEICSGEAIAPG
                     PEGIPVFDRDKCIHCGACVWNCSQPIEENPERTNITFRAGAGGLHSAQN"
     gene            complement(9759..10454)
                     /locus_tag="VFQ79_00425"
     CDS             complement(9759..10454)
                     /locus_tag="VFQ79_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEU0138137.1"
                     /translation="MNLKHTLESSFAAVAPLAEKRRSPRRAGIVHKVEVPLPPAAPTS
                     GVPLETCLRKRRTNRAFGDAGLMQSEVSQLLWAAQGISGLGGLRTCPSAGAIYPLECY
                     LVVTNVDGLRPGVYRYDPDRHLLVLTAAGSRNEKLIECCLDQECAGEGAVSIIVTAAY
                     KKMTAKFGERGIRLAHMEAGHAAQNVCLQATALGLGVIALGVFDDAALAAALELPESR
                     QPLYVIVAGRKFV"
     gene            complement(10777..11238)
                     /locus_tag="VFQ79_00430"
     CDS             complement(10777..11238)
                     /locus_tag="VFQ79_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0138138.1"
                     /translation="MSEFERTATVEAPAADVFRFVSNVSNFPLFVPSVQDASAESGDR
                     VRMRGVSGGHAYDAVAYLRTDADQKRLEWGSENNARYHGWLEVKGGDATPDLSEVTIH
                     LYLGQMTNSEQSAEDQPNDFEERMQGALADSLASIRKHVVDRHVSRSAPPS"
     gene            11360..12223
                     /locus_tag="VFQ79_00435"
     CDS             11360..12223
                     /locus_tag="VFQ79_00435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0138139.1"
                     /translation="MKEDLLSLLPEREEDRRQFLVTKLAVGFALAVRPVTAETITTDT
                     QGLEAGEVQIRVADGEIPGYRAMPAKGSNFPVVLVVQEIFGVHEHIKDICRRLAKRGY
                     LAVAPELYARQGDVSNIQDIQQIISTVVSKVPDSQVMSDLDHTAAWAKKSSKGTAGKL
                     GITGFCWGGRIVWLYAAHNPELDAGVAWYGRVVGQPSELQPKYPLDIAGQIKAPVLGL
                     YGGKDQGIPLDTVDRMRAALKQANSKSEIVVYPDAPHGFHADYRPSYRKQDAEDGWKR
                     LLAWFKNHGVA"
     gene            12262..12603
                     /locus_tag="VFQ79_00440"
     CDS             12262..12603
                     /locus_tag="VFQ79_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0138140.1"
                     /translation="MQDTLPRWLAEFRKKQPEVWQAFEALGQACHESGPLDEKTRRLV
                     KLGIAIALRQEGAVHSAVRNAVSSGITEAELYHAAILSITTIGWPGAYAAMTWIEEAL
                     VRRGTEDIPES"
     gene            complement(12613..13311)
                     /locus_tag="VFQ79_00445"
     CDS             complement(12613..13311)
                     /locus_tag="VFQ79_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138141.1"
                     /translation="MASEPVAAAPTGKKKVSPIVWILAGLVGFFLLIGILVVGAGFFV
                     VNKVKNAGFDTELMQKNPVLGAAKIAVALNPEVEVLNVDEEKGTLSIRDKKTGKVITM
                     NAEDVKDGKITFSDESTGESVSFGADRAAKLPDWVPTYPGSSPQGTFSASGKEGAGGM
                     AHFTTSDPPSKVLSFYQEALKGTGYEVTMTSSSEASGGIVSAENKSARHTVLVTAAAG
                     DDGTTVSLTYGSKQ"
     gene            complement(13440..>14093)
                     /locus_tag="VFQ79_00450"
     CDS             complement(13440..>14093)
                     /locus_tag="VFQ79_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0138142.1"
                     /translation="PLRIDPNCAWSIETSVRVGESLREELSNGGYLEDPAATLEGMAE
                     VRRRLLAAGNATPLASNVAVTCFAQVPEAERLDAVQIVLSDPHYWGGIRKLQQLAHLC
                     EVLGMGLSMHSNNHLAVSLMTMVHAAAAAPHLTYACDTHYPWQTERDEVVIGGRIKFT
                     EGKVKVPDKPGLGVDLDYDRLARLREIYDRLPYRKRDDEAEMRAHVDPNWKRILPRW"
BASE COUNT         2857 a         4189 c         4269 g         2778 t
ORIGIN      
        1 cttgcggctc gaacggcttc gcttccaggc aatgcttcag aaccgggcgc ccgcgtttct
       61 tcgcgatttc cacgtcggca tcgcaggcgg cattcaacgc atcggcgtac cagcggagcg
      121 tctgcgtctc ctcgatcgcc ccttgcacat agtaaccaag cgacccgggc cagtagaccg
      181 caaactgcgc acccagctcg tgcccgattt tgacggtatt gatgacccgg aacttcgcat
      241 actcccggac ctcgggagct tccgcggcag gactcgcagc ccacacggcg tgatggaacg
      301 tctccgttgt gaccatggag cacttcacat cgttttcctg gagcgccttg ttgatggacg
      361 caacgatctc ccactgctta tcggttccga ttgcgctggt ggggatcacg tccgtatcgt
      421 gcgtggtcca gtggccgatt ccgatctcgc cccacttgcg tatgatctgc tcgaatccaa
      481 cttcctgccg tgtcggagcg tgaaagagcg cctgcccctg gtatgcagcc gcccactgcg
      541 ggccggaaat gaaatatttg cggcggatct ccggcgaata cgagcctccg cacgccgtgt
      601 ataacctttc gacaagcgct tgctgctgct gaatggggac aggtgccatg tttgagttcc
      661 ttgaactgcc catcataatg gatttaggcg aagactgctc atcgggctat ttgatagaat
      721 cgtcatcgct aacacacaga ggagcacgct tttaatgact gaaacgtcta cttacgcgcc
      781 aaaccctgaa ttcgtggcac aagccaacgt ctccggcatg gaggcttatc gcgaactgta
      841 caggcgggcg gaggaaacgc ccgaagagtt ctgggctgaa gtcgcggaaa gagagctcga
      901 ctggtttgag aaatggtctc atgccttcga gtggaatcca cccttcgcga aatggtttgt
      961 gggtgcaaaa accaatgtgg catataactg cctggaccgg cacctgaaga acggccgtgc
     1021 acagaagatc gccatcctat gggaaggcga acccggcgat gaacggaaga tcacttacca
     1081 ggagttgcac tcgatggtct gccggttcgc caatgttctg aagaatcgcg gctacaaatc
     1141 gggagaccgc gccatcatct acatgcccat gattccggag ttgcctgtgg ccatgctggc
     1201 ctgcgcgcgg ctgggcatca cgcacagtgt tgtgtttggc gggttttccg ccgaagcttt
     1261 gaaggcacgt atcaaggacc tgggcgcaac ggtggtgatt acggccgatg gtggttggcg
     1321 cagggcaaag gaagtcaagc tgaaagcggc ggtggacgag gcgctcctcg aatgtcccga
     1381 tgtccgcgac gtcattgtgt ggaagcggac cggctcagat atccccatga aggacgggcg
     1441 tgaccactgg tggcatgacc tcgaagaagg tgtttccagc gaatgtccgg ccgagcccct
     1501 ggatgccgaa cacccgcttt acgttttata tacgtcaggc acgacaggac aacccaaggg
     1561 cattctccac acgacgggcg gctacatggt tcagaccacc gcaaccatgc ggtggatatt
     1621 cgatctcaaa gagaatgacg tgtactggtg ctcggcggat atcgggtggg tcaccggaca
     1681 cagctatatt gtctacggcc cgctcgctgc cggggcgacc acggtgatct acgaaggtgc
     1741 tccggatacg cccgcgtttg accgctggtg gagcattgta gagcgccatc gcatcacaat
     1801 cctgtatacc tctccaacgg ccattcgcgc gctgatccgg cagggagacg actggcccaa
     1861 caagcacgat ctgtccagcc tgcggctgct cggttccgtg ggtgagccca tcaatcccgc
     1921 ggcatgggag tggtatcacc gcgtcatcgg aaaacagcgg tgtccgattg tggatacgtg
     1981 gtggcaaacc gaaaccgggt ccatcatgat tgcgcccatg cccggtgcga ttccggcaaa
     2041 gccagggtca ggcacattgc ccttgccggg tgtgctgcca caggttgtgg acatgagcgg
     2101 aaagccggtc ggcgtggatc aggaaggttt tctggtgatt cggaggcctt ggcccgccat
     2161 gatccgcaca atctggggcg atccgaagcg ctatgaatcg cagtactggg gccgggtgcc
     2221 gggcgtgtat ttcaccgggg acgcggcgcg atgcgacgca gacggatact attggattct
     2281 tggccgtgtc gatgatgtga tgaacgtcag cgggcaccgg ctcagtacga tggaagtgga
     2341 gtccgcgctg gtgcgccatc ccgctgtggc ggaggccgcc gtggtgggaa aaccgcacga
     2401 gattaccggc caggccgtct gctgcttcgt cactttgaag cagggcaatc acaatcacaa
     2461 cgaactggga gcggagttgc gaaaatgggt cgcgcacgaa attggagcgt ttgcccgtcc
     2521 ggaagagatc cgcttcaccg aggcgctgcc gaaaacgaga agtggaaaga ttatgcgacg
     2581 cctgctgcgg gagatcgtca cgacgcgcac ggtaacaggg gacgtcacca cgctggagga
     2641 cctgaccgta gtaacacggc tcgccgcaca gcgcgacgag gagtgatacc ctcaagttcc
     2701 ggccgtcctc agccgataaa aagggtgtgt cacaggtcgc cgcgctccat tccgactatg
     2761 tgggcttggc tcttgggagc ctgaaaaggc tcccgccatt ctcaccggtt ctcaaccgcc
     2821 tcctggcatc tcttgccaac gaagaagtat ctttcggcag gatggccgag ttgattgaaa
     2881 aagacaccgt gctgacaggt catgttctga gcatggtgaa ctcggcggca tatgctcgtg
     2941 cggcggcgat tgtttccgta cggcatgccg tttccttatt gggcgtactg aagcttcgga
     3001 atctcgttct gggcctctcc atctcccgtt tatgggcaaa tgtccagacg ccgcccggct
     3061 ggtcccatgc cagattcaat cttcactcca tcacggcggc gacgctggcg gatctactgg
     3121 tgcagaaggt acctgtagct ttcccggagg gcgcttttat tgccgggttg ctgcacgatt
     3181 ttggaaaact gctgattgcc actgccctgc cgcgcgaata cgggcaaatc caacaattaa
     3241 ggcgctccgg tgcagccgag atcgagtgtg agatggaggt actcggcatc acacatgccg
     3301 aactctctca actggcgcta cgagcctgga acgtgcctcc cgcgatacaa gacgccgtgc
     3361 ggcggcacca cgatttacgg cccatcggat cgagtttaga tctcgccgca gtgatcgggt
     3421 gcgccgatgc ctatctgaat gccaccggca tgggaatgag ttcatccacc ccatccgaac
     3481 cggaaaccgc agttgccctg ctggagcccc tcgaaatcaa cgattctctt cccgccgtgc
     3541 tggatctgtt cacgacggag gtcgaggcga tccgaagttt cttctaattc agctcggcgg
     3601 cgtagacctg agtatgccct gtcttgtcgc tcgcgaaagc caccagtttc tcgtcatggg
     3661 agaacgaggg gtgcggatgc gtccactgcg gcccgtatac ggtatccgta gataactcca
     3721 tccaactcaa agttccgctg ccaagaacgc tgcgcgcggc ggcgaagtct tccgccaatg
     3781 cgtaccggga tgtcttccat tggctgccct gactgctggc ctcggagaga caaacccgat
     3841 tccgctctcc ggtggccaca tccaccagga acaatccatc gtcgggatga ttcgtgtcgc
     3901 acagcacgcg ggaaccggcg cggtcgggcg tgatgtgcca ggcattaaat tcggcgataa
     3961 ccgtcacttc ccgcgtatcc cagttcagcc gcttcaaagc atagggccac acggtaaaga
     4021 cgatgttacc ggtgttgccc aggaacgtct cgtgcaccac gaattcgtcg ttgccgtgct
     4081 gatagaggca ttccaggctg ctgccgtcgc gacgcacacg gtgcatgcgt ggagcgggat
     4141 ctcccgcaaa ctcgagccat tcgctgtcca gtggatgaaa ctgcggatga ataaccgtcc
     4201 tcgggaacgg gatcagattc cagccggtcc catcggtcct tcccaccaca acgccgtttt
     4261 gcccgagctg tttgatggcg gcggtaagcc atcctccgtc acggctcagc gaacattcac
     4321 cgagttgcgc ctcttcgaac gtgacaatac atcgttcttc cagcgttttc cggtcgatgc
     4381 tccaaaccga gcccccgcgc acaaagaaca cggttgcgcc gtcaggatga atggccggtg
     4441 aaaacgcgtg aatcggcagt cccgccgtga gttgccggat ctctccgtcc ggaaacgcgg
     4501 cttcaaataa ctgtgcgctg ccggttcgat aggaagtaaa taagatgtgc ctgccatccg
     4561 gagtgaagga actctggagg aagtacgtcg ggtgattgat cgacgggtgt gacgtgagtt
     4621 ggtgtaccag cgctccggtg acccggtcgc gatacgagga atgttcggat ggatggagag
     4681 agcctttgcc gtaacttaca ggttcacgtt cactttttgg cagcattggc aaattgcacc
     4741 tcaatcggaa tcgtgtggcc gagcggctgg acgtgcagcc tgatcgtaat ggacgaaccg
     4801 ggcgcctttt cacccggtac agaacggatc agaatcttgg ctgagccgcc acgcgccact
     4861 tcttttgtgt cgagcgacgc ttcgaaaccg gccgccggag aatactcgag cgagagagcg
     4921 atggcaccgg acattccatt cgagacgtga atctccgcgg tgggagattc gccgctcaat
     4981 tgtacttttg ttttatcgac actcaccatt ttgatgagcg cctgcggatc cggtcccttg
     5041 ttcaggaaga ggccgcccga ggactcgccc tcgccgggct tcatacgccc gaagggagtc
     5101 tccttgccga ggttgggatc gacataccag aaccactctc tattctcgag cttccacaac
     5161 gtggtgagcg gtgccttgac ggccactttg cccattccgg ggaacgccat atccgtgtcc
     5221 accgcaacca caacccgcgc cttcgtgaac tgatccgagt aggctatttt tacaatctcg
     5281 aagcctttgt accgcgtctt ctgcgcggcg aaaaagactt ctttgctgtc ctcggcaacc
     5341 acctcatcgg cgacccggaa ttttccgtcc acgtgcgcct gatagaactt ggcgatgcgc
     5401 ccgcgcaacg ccgcatccac gtcgggcggg gccttggaga agagatcctc cgcagcctgt
     5461 ccaaaacaga gaccggtgaa tacacaagct ccagctagta caaatcgaag catcatctgt
     5521 tattttacgg taccccaagc cccgttttac tctgatggtt ttcgcgcaat ctccagaaca
     5581 ccgccagcac atgggggatt tgccgcaacg atatgacgcg gagatctccc gaaatgcaac
     5641 aaaagtctac agctttgtgc ctgttcaccc attcataccc tggcatccga attgctccgc
     5701 aatcaagatc atgacaacca gcgcggtcac tcccaaaaaa gcggctctac gcaccctgcc
     5761 cggggacgac gtccggcaga tcatgtggcg cttcgccggc cggtacgaac tacagatgct
     5821 ggtacaggcc tcccggtccg cggcccgcgg tccggttgcg cggctggtgg ccgaaggtgc
     5881 gcggaatacg catgaatgga cccgggagaa ggcgcagctt ctcaccgcct tcgatgaggc
     5941 gggcatcacc agcgtcttca tggaccccga acaaggcggg ttcattgaag gaccgaagaa
     6001 cctggcgctt gcgctggttg cgttcgagct cgcctgggta gacgcaggcg ccgccacggg
     6061 cagcctggcg ggatgcctgg cgctcgcacc gattcacgaa aggggcacgc cggcgcagcg
     6121 cgacgaatac atgaagcgat gcactcctcc cgcgccggga gaggaccgca agccctggcg
     6181 gggcgctttt gcgctgaccg agcccattcc ctacgtgggc gtggaaacag gactcctcag
     6241 cggcaaagtg cgtgttgtgg agtggcccga tggcggcgaa cccattttgc aggtggacaa
     6301 gcgtgggcgt ttcatcacca acatgggctt tgcgaacttc gtgacggcgg cggtggattc
     6361 cggggacgag cgcattaaag gaagctcgat gatcatcctg gaagagaccg atccggggat
     6421 tttcgaccgc ggaattccta cccggaagct ggtccaccaa ctctcctcaa cgcgcgatcc
     6481 cgtgctgagc ctgcgcgtgc ccgccagccg catcattggc ggctacacaa taaaagacgg
     6541 cgtcatcgtg ccgcgctata gccacggcga gatcatcgaa gcggtattcc gccggacgcg
     6601 cgtcacggtc gggctgatga cttcggcgaa gctgttgtcc gccgtcgaac cggttctgcg
     6661 gtatcagcgg caacgcttcc gcggcgcgga cgcggtggcg ccgggttcgc cgcggtatga
     6721 gctcgggttg cagcagcgcg aagattcgct gcaccggctc atcgatgtgt gggcgactgg
     6781 cgaagcgagc gcatcgctgg gttttgccgc cgcgcggctg ttcgacgaac tggacccgct
     6841 cgaacgcgaa aaagaccgtc ttctcgagga acagggaatc ctgggcgggt cggcccaatt
     6901 caaagcgctc cggaagcggc aaaacgaagc gctccaggcg atctcttccc gcgtgatgcc
     6961 ggaggatccc ctgctccgct tccttgtgct cgattcagtg gccaatgtgt tatgtcccgc
     7021 cacaaaacta tggaacaccg gccatggcgc caacatgatg cgggaagcgg tgagcctgat
     7081 ggggggctac ggaatcaccg aggattgccc gggcttcctt ggccacaagt ggatggacgc
     7141 ccagttggaa gccacctatg agggtccgga ggcggtgcag cggcgtcaat tggccgtcac
     7201 catgacgaac gagctgttcc tgaaacagta ccggcaatgg attgcggcga tgcgcgagat
     7261 cgcctccagc aaacccggaa ccggggcttg cactctcgcc accgccatgc agatgtggct
     7321 gtggacgctc gagcgcttgc agaaatcgag cgatgccgag ggatcgaagc tttaccagaa
     7381 tgcgcgacag ggagtcacct tcgcactggc cgacgcgctg tgctgggttc ttgcggcacg
     7441 gcagatgatc ctcgatcttc tcgaactaga ggccaaggga ccggaaaatc cgactgttgc
     7501 ggaagcgttg ccgggcctgg cggcatttta caccgacctc gcccacgtcc aatcggcaag
     7561 atccgccggg gaagtggggc gaatctgtgc agaactcgtc ttcggctaca accgtcatcc
     7621 ggcttgggac gagcagggtt gcggaagctg ctatagctcc gacgacctaa cggcattgga
     7681 gaacttgatt ccgggcatcg ccagttgcgc cgtcgacgtg gtggaaagcg acggatcgca
     7741 tcccatgaag gcgggccctt gtgtgcgctt cgacggcatg gagcaattca cccggctgcg
     7801 aacgcgtctg gacggttgcc tcaccggcgc acggctcgca aaagaccgtg ccgcggaagc
     7861 gcttacccgg gtgatgatcc ccgaggcact cgactactga gagcatgaac gtcgaacgcc
     7921 agagcatgga cgtggacatc gtctgcgtgg gtttcggtcc cgcggcgggc ggatttttga
     7981 cgactctttc ccgcggcatc ctgaatccgg acggaacgcc ggccgtggaa agcagcgcga
     8041 tgccgggaat gcctccgcag gtggtctgtt acgagcgcgc ggacgatatc gggttcggcg
     8101 tatcgggtgc cgtcacccgg gcgcgcggca ttcgcgccag cttccccgat ctcgatctat
     8161 cgcagattcc gatgtccgcg cgcgtggaga aagagaaagt tctctatctg ctcgatccgg
     8221 tgggcgccag ccgccgctcc cgtgccttgc gggcggcgga tgccctgatt cgggggttcc
     8281 gctttgcttt accgctggaa tgtgatgcgc tggagctgcc ctatacaccg cccttcttgc
     8341 gtaaggacgg cgggcttttg ctctcgatcg gccagttcaa ccaatgggtc ggccaatcgc
     8401 tgatggcgga gggtgccgtt caaatctggc cgggctcacc ggtatcgcag gctctggtcg
     8461 agaatggcca ggtgaccggc gtcaggctgc tggaccaggg caccgataaa tccggcaacc
     8521 ccgaggcggg cttcctgccc ggcatggatg tgcgtgcggc tctgacggta atcgccgacg
     8581 ggccggtggg tgccgtgggc cgccagatgg acgagcaatt cggaatgccc gacggccatt
     8641 cgcggcagga ttgggcggtc ggcgcaaaga tggtgatcga gcttccggag gatacacccc
     8701 tcgagacggg caccgtcctt catacgttcg gctaccccga acccgaaatc ttcggattcc
     8761 tctacgtcta tgcggaccgt ctcgcgtcgg ccggtatctt tgtgccttcg tggttcgata
     8821 acccggtccg tacttcgtac cggtatttgc agcattttct tttgcacccc tatctgtggc
     8881 gctatgtaaa gggaggacgg ctgctctcct ggggcgcgaa aacactccag gagtccggaa
     8941 gacgcggcga gccctacctg gcgggggatg gatacgcccg catcggcgag ggttccggca
     9001 gcacgaatgt cctgacggga tccggcgtcg atgaagcgtg gaccactggc gtcctgctcg
     9061 gcgagtcggt gctggaacta ctcaagcagg gccggccatt cacgcgcgat aatctcgatt
     9121 ccacctatgt cgccaaacgg agacagagtt gggtggagcg cgagggtaga atcgccgaaa
     9181 aagcgcgaga cggttttcac aatggcgtgg tcacggggtt aatcggcatg gcccttaccg
     9241 gtctcaccgg tgggcatctg gcgatgcccg gaaaaccgag gccggtctac gaagatctgc
     9301 ccgacgtcta cgattactac aggaaaaaga tcggcgcgga agaaatggcg cggattcgcc
     9361 gcgaatgcac ggagaagcgt ctgccgctgc acgacaccct gatggagcgg tgcggctggc
     9421 ccaaaatccc gtacgacgga aagctgttga tctcgcacca ggacgcgctg ctggtgggcg
     9481 gcaaagtgca ggcggctccc ggttacgccg atcacgtggt gtttctgtat ccttcgctct
     9541 gcgaagcctg cggaacacag atttgcgtgg agatctgctc cggggaagcg atcgcgccgg
     9601 ggccggaagg cattcctgtc ttcgaccgcg ataaatgcat ccactgcggt gcctgcgtgt
     9661 ggaattgctc acagccgatc gaggaaaacc cggaaaggac caatatcact ttccgggccg
     9721 gagccggtgg gctgcactcg gcacagaact gagccgtatc agacgaactt gcggccggcg
     9781 acgattacgt aaagcggctg gcgggattcc ggcaactcga gagcggcggc gagcgccgcg
     9841 tcatcgaaaa ctcccagggc aatgacgcca agacccagtg ccgtggcctg taaacagacg
     9901 ttttgcgccg catgtcccgc ttccatatgc gcaagccgga tgcctcgctc tccgaacttc
     9961 gccgtcattt ttttgtacgc cgcggtgacg atgatggaaa cggcgccttc ccccgcgcac
    10021 tcctggtcga ggcagcattc gatcaatttt tcattccggc tgcccgccgc agtcaggaca
    10081 agcaggtggc gatccgggtc atagcggtac acaccgggcc gcaagccgtc gacattcgtg
    10141 acgacaagat agcattcgag cggataaatc gctcccgccg agggacacgt tcgcaaacca
    10201 cccagaccgc tgataccctg cgccgcccag agcagttggc tcacttccga ctgcatcaac
    10261 cctgcatctc cgaatgcccg gttcgttctc cgctttcgca ggcaggtttc aagcggaacc
    10321 ccggatgtcg gcgcggccgg aggaagaggc acttccacct tatgaacaat tcccgctcgc
    10381 cttggggaac gccgcttctc cgcaagaggc gccacggcgg caaatgatga ctccagcgtg
    10441 tgtttcagat tcactattac agtctaacag gccggaatat cctggctatc tgccctgaaa
    10501 cggatcaagc agacgggaaa agagacctga ggtttggcgc ttaccccacg caacgcaagc
    10561 tcccgaatca aggcccgttc atagaccttc tccaggaacc cggcaccaag cacattggca
    10621 acctcgcagg ccgacccaat cacggcttcc gtcagttccg attccgatcc gtgtgcatcc
    10681 gtggcccttt tcaatggggt tgcagcaacg gggcaaaagt cccagcgaca ggatggaaaa
    10741 tcgccacaga tggacgcgga tgcacacgga ttatcactaa cttggcggcg cggatcggga
    10801 aacatgccgg tccactacgt gcttgcggat cgaggccagg gaatccgcca gtgcaccctg
    10861 catcctttct tcgaagtcgt tcggctggtc ttcggcgctc tgttcgctgt tcgtcatctg
    10921 cccgagatag aggtgaatag tgacctcgga aagatcgggc gtagcgtctc cgcctttcac
    10981 ttccagccag ccgtggtatc gcgcattgtt ttccgaaccc cattccaggc gcttttggtc
    11041 tgcgtccgtc cgcaggtagg caaccgcatc gtaagcgtgt ccccccgaaa cgcctcgcat
    11101 acgcacccga tcgcccgatt cggcggaggc atcctgtaca gaaggcacga acagaggaaa
    11161 gttggagacg ttcgatacaa accggaaaac atcggcggcc ggagcttcta cggtggctgt
    11221 acgctcgaat tcgctcataa attcagtcct caccggataa gacaaagtgc ggcaaccggc
    11281 cgtttcaaaa tcaacgaaac gccgggatac ctcggggcgc ctcgcccggt ccgctacact
    11341 ctttgaaggg agggatgcga tgaaggaaga cctgttgagc ctgcttccgg agagggagga
    11401 agaccgccgc caatttctgg taaccaagct ggctgtagga ttcgcgctgg ctgtccgtcc
    11461 ggttaccgcg gagaccatca caacggatac acagggactg gaagccgggg aggttcagat
    11521 tcgcgtggcc gatggcgaaa taccgggtta ccgcgccatg cctgctaagg gttcaaactt
    11581 ccccgttgta ttggtggtgc aggagatctt cggcgttcac gagcacatca aagatatatg
    11641 ccggcgcctc gccaagcgcg gctatctggc tgtcgccccg gaactgtatg cgcgccaggg
    11701 cgacgtctcc aacatccagg acatacagca aattatctcc accgtggtat cgaaggtgcc
    11761 cgacagccag gtgatgtccg atctcgacca tacggcggca tgggcaaaga aatcgagcaa
    11821 aggtaccgcg gggaaattag ggatcacggg attctgctgg ggcggccgga tcgtctggct
    11881 ttacgccgcg cataacccgg aattggatgc gggcgtagcc tggtacggca gggtggtggg
    11941 ccagccgtcc gagttgcagc cgaaataccc gcttgatatc gccgggcaga tcaaagcgcc
    12001 ggtgttgggt ctctacgggg gcaaggacca gggcattcca ctcgataccg tggacaggat
    12061 gcgggcagca ttgaaacagg caaactcaaa atccgagatc gtcgtctatc ccgacgcgcc
    12121 ccacggcttc catgccgatt accgtccgag ctaccgcaaa caggacgccg aggacggctg
    12181 gaaacggctg ctcgcctggt tcaaaaatca cggcgtggcc tgaccggcgc ttcgctgaat
    12241 agaacaaaag gggaatgagt tatgcaggat acgcttccgc gttggctggc ggaattccgg
    12301 aaaaagcagc cggaggtctg gcaggctttc gaagcgcttg gccaggcttg tcatgaaagc
    12361 ggaccgctgg atgagaagac caggcggctg gtaaaactcg gaatcgccat cgcccttcga
    12421 caggaaggcg cggtacactc ggcggtccgt aatgcggtaa gctcgggcat cacggaggca
    12481 gagttatatc atgccgctat cctatcgatt acaacgatcg gatggccggg cgcctatgcc
    12541 gcgatgacgt ggatcgagga agcactcgtg cgccgcggca ccgaagacat cccggaaagc
    12601 tgatgtgtcc tactattgct tggaaccgta ggtcagactg acggtggttc catcgtcgcc
    12661 ggcggcagcc gtcacgagga cggtgtggcg ggccgattta ttctccgcgc tcacgatacc
    12721 gcctgaagcc tcggaactgc tcgtcatcgt aacttcgtat ccggtgcctt ttagcgcttc
    12781 ctggtaaaag gagagcacct tcgagggcgg atcgctcgtc gtgaagtgag ccataccacc
    12841 ggcgccttcc ttgccggagg ctgaaaacgt gccttgcggc gacgacccgg gataagttgg
    12901 aacccaatca ggcaatttgg cggcccggtc cgcgccgaag gagaccgatt ccccggtaga
    12961 ctcgtccgag aacgtgatct tgccatcttt cacatcctcg gcgttcatgg tgatcacctt
    13021 gccggttttc ttatcccgga tcgacagggt gcctttctct tcatccacgt tgaggacttc
    13081 cacttccgga ttgagcgcga cggctatttt cgcggcgccc aggacaggat tcttctgcat
    13141 tagctccgtg tcaaagcccg cgttcttcac tttattgacg acaaagaagc cagcacccac
    13201 aaccagaatc ccgatcaaca ggaagaaccc caccagcccg gcgagaatcc agacgatcgg
    13261 actgaccttc ttcttgcccg tgggcgccgc cgcgacgggt tcgcttgcca tccgtgcgcc
    13321 acagttctta cagaaagcac cttcattcgg agtgccgcaa ttcgtgcaaa aggccatagt
    13381 atcctcagtg caaggctatg ctaaaagctc tcgcttgtcg aggggcttgg tttgcgggtt
    13441 taccagcgcg gaagaatgcg cttccagttc gggtccacat gcgcccgcat ttccgcttcg
    13501 tcgtcccgtt tgcggtaagg cagcctgtcg tagatctcgc gcagacgcgc gaggcgatcg
    13561 taatcgagat caacccctaa accgggcttg tccggaacct tcaccttgcc ctctgtgaac
    13621 ttgatccgtc cgccgatgac cacttcgtcg cgttcggtct gccaggggta gtgcgtgtcg
    13681 caggcatagg tgaggtgggg cgccgcagcg gccgcgtgga ccatcgtcat caggctcacg
    13741 gccaggtgat tgttggagtg catggaaagg cccatgccaa gcacttcgca caggtgcgcc
    13801 aactgctgaa gcttccggat gccgccccag taatggggat cggagagcac aatctgcacg
    13861 gcatccagcc gctccgcctc gggcacctgt gcgaaacacg tgacagcgac gttgctcgcg
    13921 agcggagtgg cattgcccgc ggccaggagg cgccggcgga cctccgccat gccttccaac
    13981 gtcgcggcgg gatcttccag atatccgccg ttcgacaact cctcccgcaa gctctcgccc
    14041 acgcggacgg atgtttcgat agaccacgcg cagttcggat cgatacgcag cgg
//
LOCUS       DASTMK010000013        10083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_320696, whole genome shotgun sequence.
ACCESSION   DASTMK010000013 DASTMK010000000
VERSION     DASTMK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320696"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFQ79_00455"
     CDS             complement(<1..451)
                     /locus_tag="VFQ79_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138143.1"
                     /translation="MIPRFFVLICLLSLPVSAANPELATQFWSAHWISVPGTPPSAYG
                     VYHFRRAFELASKPGSFLIHVSADNRYQLFVNGERTAWGPARGDLFHWRYETVDIAPL
                     LRPGRNVLAAVVWNFGEHAPQAQVTNETGFLLQGDTERERIADTNAEW"
     gene            complement(465..1316)
                     /locus_tag="VFQ79_00460"
     CDS             complement(465..1316)
                     /locus_tag="VFQ79_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0138144.1"
                     /translation="MLTRRFGWTGLTLPAIGQGTWKIEGSGEKERQAIEALQTGIEMG
                     ATLIDTAEMYGNGRAEELVAEAIRGVRDKIFLVSKVLPWNASYEGTLKACARSLKRLH
                     TDYLDLYLLHWESDHPIGETMRALGKLVRDGSIRLLGVSNFDVPKLKEAQAALNNERI
                     ACDQVLYHLGDRGIERTVLPYCREEEIAVMAYSPFGSGQFPTSRSRGGRVLSEIGARH
                     KRSPRQVVLNFLLRRSEVFAIPKAGNTEHARENASASDFELSQEEMERIDRAFPAPDR
                     DTPLGMI"
     gene            complement(1323..1967)
                     /locus_tag="VFQ79_00465"
     CDS             complement(1323..1967)
                     /locus_tag="VFQ79_00465"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU0138145.1"
                     /translation="MFTGIIEELGAVEGMEPRDAGMRLRIRCRKVLEDTREGDSISVN
                     GVCLTATAIRPGSFGADVSPETLRRSNLGDLRLNAPVNLERPLSPGGRLGGHIMQGHV
                     DATAEFLSLDALGGDNWWLNVRVPAEVERYLVFKGSIGIDGISLTIASVEDSIVSATI
                     IPHTYRHTTLGTYSPGNRVNIECDVIAKYVEKMLSNLTIPRSGLTIERLKEEGY"
     gene            complement(1971..3077)
                     /gene="ribD"
                     /locus_tag="VFQ79_00470"
     CDS             complement(1971..3077)
                     /gene="ribD"
                     /locus_tag="VFQ79_00470"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU0138146.1"
                     /translation="MNNDYMEAALALAREGLGQTSPNPTVGAVLVLDGEIVGRGFHTY
                     AGVKHAEVLALEEAGARARGAALYVTLEPCCHTGRTGPCTDALIAAGVKRVVAAMEDP
                     NPQVRGEGLRRLREAGIAVDLSFAHASEAAKLNEPFVHFMRTGLPLVTLKAAVTLDGK
                     IAAPEDNRGWITSERAREHVQELRHLSDAIVTGIGTVLGDDPLMTDRSGKPRSRPFLR
                     VVLDSQLRIPPDSRMVAGCRSDLLIVTTSAASSERRKVLEERGCSILVADGPGGRTDL
                     RALLDYLGRENRYLSLMIEAGSKVNWAALESGVVDKIFFYYAPKILGGLHSLPVVGGA
                     GRLRRVDAIRFRNVSLHSIAPDEFAVEAYFVKEN"
     gene            complement(3074..4024)
                     /gene="ftsY"
                     /locus_tag="VFQ79_00475"
     CDS             complement(3074..4024)
                     /gene="ftsY"
                     /locus_tag="VFQ79_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU0138147.1"
                     /translation="MIQTLFGSVEQEPNLLEKLKMGVQKTRSGLMTRLEDTFAGAKEI
                     DADVLEELEYSLITADIGVKTASEILDRVRERVDRRMLGDAGELKLLIRQHLFEVLEA
                     TERPPVRVMEPPAVILVVGVNGSGKTTTIGKLAGRFKAEGKTVLLCAADTFRAAAIEQ
                     LEVWGDRTSTEVIRQQPGSDPSAVLFDALHAAKARKTDYVIVDTAGRLHTKSNLMAEL
                     EKMDRTAKRVIPSAPHEVLLVLDATTGQNGLEQARRFTETSGVTGIVLTKLDGTAKGG
                     IVVAIARELNLPIRYIGVGERAEDLLPFDAEKFIASIFEA"
     gene            complement(4090..4452)
                     /locus_tag="VFQ79_00480"
     CDS             complement(4090..4452)
                     /locus_tag="VFQ79_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0138148.1"
                     /translation="MPTKPYTLITDLVKQADVPENGILSRTLQNDDKSKVILFAFADG
                     QELTAHTAPMPAVIYLLEGSAEVTLGEDVMNLEGGALIHMTPNLRHGIRAKGPVKMML
                     ILLKDASPAASEHQGPPA"
     gene            4516..5079
                     /gene="nadD"
                     /locus_tag="VFQ79_00485"
     CDS             4516..5079
                     /gene="nadD"
                     /locus_tag="VFQ79_00485"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU0138149.1"
                     /translation="MRTAIFGGTFDPIHCAHLRVAREALEQFHLDRVLFVPASTPPHK
                     REATAGYEDRYRMVELACANEPRFVPSRLEAGRLKSYSIKTIRRVRASVARDDELFFL
                     IGSDAFAEIDTWYRAADVIRAVDFIVVTRPGHEYIVPPGARVHRLDTLALPVSSSDIR
                     RRLSAGESPEELQPKVLAYIREHHLYE"
     gene            5117..6535
                     /locus_tag="VFQ79_00490"
     CDS             5117..6535
                     /locus_tag="VFQ79_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0138150.1"
                     /translation="MEFPMHIGGELVRTDKPQQVTLPYDGSYAGTVFEAAREQVDAAV
                     EAAAAAAPAMREMSRDERSAILRRAHYKLLEQKQEMARAISSECGKPLREALAEVDRA
                     AATLLFSSEEAHRLSGEVVPMDASPGGKGRWGMTVREPLGVVAAITPFNFPLNLAMHK
                     IGPALAAGNAVVHKPASTTPISGLRMAGIFETCGLPKGALNVITGPGGTIGDLFTFHK
                     NVAMITFTGSTDVGLRIRNLAGLKRVTLELGNNSAVIVEDDANLDEAVPRCVTGSFAH
                     SGQVCISVQRIFVHEKIRKEFVERFVAAAGKLKIGHPLDPETDISSLITEKEAQRVES
                     WVDEAVKAGATLVRGGSRRRATMEPAVLTDVPPDAQLSCREAFGPVVGINPYTDLDEA
                     IEQVNRSEYGLQAGIYTRDLEKAFRAARRVHVGGFMINEVPQYRADQMPYGGVKMSGT
                     GREGPKYAIEEMTEVKLICWKP"
     gene            complement(6542..8110)
                     /locus_tag="VFQ79_00495"
     CDS             complement(6542..8110)
                     /locus_tag="VFQ79_00495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU0138151.1"
                     /translation="MSDQGAPPPLPPKEKFDGEQPLRAGILSEAFYIEDQHERDKLGM
                     PPDDLTPTSVVVELNLFHEKGLPGSRERFQQIHDALAARYEKKPPLLIANTYYRCYLS
                     ISDARDLASRDQSETDSKRRCIYRIWPDFRLMPLINRSVATVKADAARRAYDASGSEI
                     VWAVIDSGIQADHPHFQLYETLGGEVSGLHRDFTKDEPDVEGALTDKFGHGTHVAGII
                     AGALCRPEVDGSKVEYCMGQRMAVSPDSDLERLERRTVRHPGLLSGVAPLTKLISLKV
                     LDDNGRVRSTDVLRALEYIRSDVNGHGKPPLRVHGVNISLGYEFNAKWFACGQSPVCI
                     EVDRLVRSGVAVVIAAGNTGYGELMAKAGPTNTGLSLTINDPGNAALAITVGSTHKDS
                     PHTYGVSYFSSKGPTGDGRLKPDLVAPGERIVSCATGKKLELMKRQKLLDGVCSLEEG
                     KHPAAYIDESGTSMAAPHVSGAIAAFLSIRREFIGKPEDVKRIFLNSATSLGRERYFE
                     GSGLVDLMRAIQSV"
     gene            8344..8595
                     /locus_tag="VFQ79_00500"
     CDS             8344..8595
                     /locus_tag="VFQ79_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU0138152.1"
                     /translation="MAYVITDTCTKDELCVDACPVDCIHPKKDDAKFEEVPMLYVNPD
                     DCIDCGACVPVCPTSSIYVLEELPEDKAHFAAMNAAYYN"
     gene            9376..>10083
                     /locus_tag="VFQ79_00505"
     CDS             9376..>10083
                     /locus_tag="VFQ79_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138153.1"
                     /translation="MTPAGVVSVMAGSGSLTRLDAPPNRVAVDRQNNIYIAGENRRIL
                     RLPANGAPTVIAGNGQFGAPAIGSPATSSALGAITALAVNDSGEIFLVDSLYHVILKI
                     GTQGNLEAVLRDADASDLAFDSAGRLLFTDTIAGRVWRVNGDGSRTSIAGRIRFEGDG
                     GPAIDALLKSPRAVAVHPDGTLFIGDTGNHRIRKVTRDGVISTVAGNGTPGVAGDGGP
                     AAAAQVAGPDHLVIDGRG"
BASE COUNT         1929 a         3199 c         2969 g         1986 t
ORIGIN      
        1 tccattccgc gttcgtgtcc gcgatgcgct cgcgttccgt gtcgccctgt agcaggaagc
       61 cggtctcatt cgtcacctgc gcctgcggcg catgctcgcc gaaattccag actacggccg
      121 cgagcacatt ccggccgggc cgcagcagcg gtgcgatatc gacggtttcg tagcgccagt
      181 gaaagaggtc gccgcgcgcg ggtccccatg ccgtccgctc tccgttcacg aataactggt
      241 agcggttgtc ggcgctcaca tggatgagga agctgccggg tttggatgcc agctcgaacg
      301 ctcttcgaaa atggtacacg ccgtaggccg aaggcggagt tcccggcaca gagatccaat
      361 gcgcggacca gaattgcgta gccagctcgg ggttcgccgc ggaaacaggg agggatagaa
      421 gacaaatcag cacgaagaaa cgcgggatca tcgtgccgat cctatcagat cattccgaga
      481 ggggtgtcgc ggtcgggcgc cggaaatgca cggtcgatcc gttccatttc ctcctggctc
      541 agttcgaaat cggaggccga tgcgttctcg cgcgcgtgtt cggtattgcc ggctttcgga
      601 atcgcaaata cctcagaccg gcggagcaga aagttcaaaa ccacctgccg cggcgatcgc
      661 ttgtgccggg cgccgatctc actcagtacg cgtccgcctc gcgaccgtga ggtcggaaac
      721 tgtccgctgc caaacgggct gtacgccatg acagcgatct cctcctcgcg gcagtacggc
      781 agcacggtgc gttcaatgcc gcggtcgccg aggtgataca acacctggtc gcaggcgatt
      841 cgctcgttgt taagcgccgc ctgtgcctct ttcaacttgg gcacatcgaa gttgctgact
      901 ccgagcaacc gtatgctgcc gtcccgcacc agtttcccca gggcgcgcat ggtctcaccg
      961 atgggatgat cgctttccca gtgcaaaaga tagaggtcca ggtaatcggt atggaggcgt
     1021 ttcaagctgc gcgcgcacgc cttgagcgtg ccttcataag aagcgttcca cggcaggacc
     1081 ttgcttacca ggaagatttt gtcgcgcaca cctcgaatgg cttccgctac cagctcttcc
     1141 gcgcggccgt tgccgtacat ctcggccgtg tcgataagcg tggcccccat ctcgatgccg
     1201 gtttgtagcg cctcgatcgc ctgccgctcc ttttcgccgc tgccctcgat tttccacgtt
     1261 ccctggccta tcgccggaag cgtcagccct gtccatccga aacgtcttgt gagcacgcgc
     1321 gctcaatacc cctcttcctt caagcgttcg atcgtgagtc ccgaacgtgg aatcgtaagg
     1381 ttcgacagca tcttttctac atatttcgcg atgacgtcgc attcgatatt cacccggttt
     1441 ccgggcgaat acgttcccag cgtcgtatgg cgataggtgt gcggaatgat ggtggcggaa
     1501 acaatggaat cttcgacgga ggctatggtc aggctgatgc catcaattcc gatggagcct
     1561 ttgaatacaa ggtagcgttc cacctcagcg ggcacgcgga cattgagcca ccagttgtcg
     1621 ccgccgagcg cgtcgagcga aagaaactcc gccgtcgcat ccacgtgccc ctgcatgatg
     1681 tgcccgccaa gcctgcctcc gggcgacaag ggccgttcaa gattcacggg cgcgttcaag
     1741 cgaaggtccc cgaggttgct tcgccgcagc gtctccggcg aaacatccgc gccgaacgac
     1801 cccgggcgaa tcgcggtagc cgtaaggcac acgccgttta ccgatatgct gtctccttcg
     1861 cgagtatctt ccagcacctt gcggcaacgg atgcgaagac gcattccggc atcgcgcggc
     1921 tccatgccct cgaccgcacc gagttcctcg ataatgccgg tgaacaacgc tcagttctcc
     1981 ttcacgaagt aagcttccac tgcgaactcg tcaggcgcta tcgaatgaag cgacacattg
     2041 cgaaaccgga tcgcgtccac gcgccggagg cgtccggcgc cacctaccac cggcagcgaa
     2101 tgaagcccgc cgagaatctt cggcgcatag taaaaaaaga tcttgtcgac caccccggat
     2161 tcgagcgcgg cccagttcac cttgctgccg gcttcaatca tcagcgagag atacctgttc
     2221 tcgcgcccca ggtagtcgag tagcgcccga aggtccgtac gtccgccggg gccgtccgcg
     2281 acgaggatgc tgcatccgcg ctcctccaaa actttgcgcc gttcgctgga tgccgccgat
     2341 gtcgtgacga tcagaagatc cgaacggcat ccggccacca tgcgtgagtc cggtggaatg
     2401 cgaagctgcg aatcgagcac gacgcgaaga aacgggcggc tgcgcggctt cccgctgcgg
     2461 tccgtcatca gcggatcgtc gcccagaaca gtcccgatgc ctgtcacgat ggcgtcggag
     2521 agatgccgca gctcctgcac gtgctcgcgt gcccgctcgc tggtaatcca gccgcggtta
     2581 tcctccggtg cagcgatctt cccatccagc gtgaccgcgg ctttcaacgt caccagcggc
     2641 aggcccgtgc gcatgaaatg aacaaagggc tcattcagct tggcggcctc gctcgcatgg
     2701 gcgaaactca aatcgacggc gatgcccgct tcgcgcaggc gccggagccc ctcgccgcgc
     2761 acctgcggat tgggatcttc catcgcggca acgacgcgct tcacacccgc ggcgataagg
     2821 gcatccgtgc aagggcccgt acgcccggtg tggcagcacg gctcgagcgt gacatagagc
     2881 gcggcaccgc gcgctcgcgc gcccgcttcc tccagcgcca gcacctccgc gtgtttcaca
     2941 ccggcgtaag tgtgaaatcc gcgtccaact atttcgccgt ccagcaccag caccgcgccg
     3001 actgtcggat tcgggctcgt ctggcccagg ccctcccgcg ccagcgccag cgccgcctcc
     3061 atgtagtcgt tgttcatgct tcgaatatgg atgcgataaa tttctccgcg tcgaaaggaa
     3121 gcaggtcttc cgcgcgttct ccgacaccga tgtagcggat cggcaaattc aactcacgcg
     3181 ctatcgccac cacgatgccg cccttcgccg tgccgtccag cttcgtcagc acgatgccgg
     3241 tgacgccgct cgtttccgtg aatcgccgcg cctgttccag tccgttctgg cccgttgtcg
     3301 catccagcac caacagcact tcgtggggcg cggagggaat gacgcgcttc gccgtgcggt
     3361 ccatcttttc gagctccgcc atcaggttcg atttcgtgtg tagacggccg gcggtatcga
     3421 cgatgacata gtcggtcttc cgcgccttgg cggcatgcag agcatcaaac agcaccgcgc
     3481 tcggatcgga acccggctgc tggcggatca cttccgtcga cgttcgatca ccccagactt
     3541 cgagctgctc gatggccgcc gcgcgaaacg tatccgcggc acagagcaga acggtcttgc
     3601 cttcggcctt gaaccggccc gccagtttac cgatggtagt cgtcttaccc gagccgttga
     3661 cgcccaccac cagaatcacc gccggaggct ccatcacgcg cacagggggc cgctccgtcg
     3721 cctccagtac ttcgaacagg tgctggcgga tcagcagctt caactcgccg gcatcgccga
     3781 gcatccggcg atccacacgc tcccggacgc ggtcgaggat ctcgctggcg gtcttcacgc
     3841 cgatgtcggc ggtgatcagg ctatattcga gttcctcgag tacatcggca tcgatctcct
     3901 ttgccccggc aaacgtatct tcgagccggg tcatcagtcc cgagcgcgtc ttttggacgc
     3961 ccatcttgag tttttcgagg agattgggct cctgttcgac agagccgaag agtgtctgga
     4021 tcataaaacc agggctgcct ttccgattgt atcagcggct acgcgggagc ccggcgagcc
     4081 ggagagggat caggccggtg gtccctggtg ttcgctcgcc gcgggggagg cgtccttcag
     4141 cagaatgagc atcatcttga cgggaccctt cgcgcgtatg ccgtgccgca gattcggcgt
     4201 catatggatc agcgcgccgc cttcaaggtt catcacgtct tctcccagcg tcacttcggc
     4261 cgacccttcg agcaggtaga tgacagcggg catcggcgcc gtgtgcgccg tcagttcctg
     4321 cccgtcggcg aacgcgaaaa gaatcacctt gcttttgtcg tcgttttgca gcgtccggct
     4381 gagaatgccg ttctcgggaa cgtcggcctg ttttaccagg tcggtaatca gcgtgtatgg
     4441 tttggtcggc atctctccag tctacgtcga tctacctgcc agcagcctca acgcggaggg
     4501 ttgtatcatt ctcgcttgag gactgcaatc ttcggtggca cttttgatcc cattcattgc
     4561 gcgcatctcc gggtagcccg cgaagcactg gagcaattcc atctcgatcg cgtgctgttt
     4621 gttcccgctt ccaccccgcc gcacaaaaga gaagccaccg ccggctacga agaccgctac
     4681 cgcatggtgg agctggcgtg cgcaaacgag ccacggtttg tcccttcgcg tctcgaagcc
     4741 ggccgcctga aaagctactc catcaaaacc atccggcggg tgcgcgcgag tgtcgcgcga
     4801 gacgatgagt tgttctttct gatcggctcg gatgccttcg ccgaaatcga cacctggtac
     4861 cgcgccgcgg acgtgatccg ggcggtggac ttcatcgttg tcacccgccc cggacacgaa
     4921 tacatcgtgc cgccgggtgc gcgggtgcac cggctcgaca cgctggcgct gccggtgtcc
     4981 tcctccgaca tccggcgccg cctttcggcc ggcgaatccc cggaagaact gcaacccaaa
     5041 gtgctcgcct acattcgcga gcatcacctc tacgagtaga cgtcagccgg ctgcatcgct
     5101 gttctatcat tcagctatgg aattcccgat gcacatcggc ggcgaactgg ttcgcacgga
     5161 caagccgcaa caagtcacgc ttccctacga cggatcgtac gccggcaccg tcttcgaggc
     5221 ggcacgggag caggtggatg ccgcggtgga ggctgccgct gccgccgctc cggcgatgcg
     5281 cgagatgagc cgggacgagc gctcggcaat cctccggaga gcacattaca agctgctcga
     5341 acagaaacag gagatggccc gcgccatctc ttcggaatgc ggcaagcccc tccgggaagc
     5401 ccttgccgaa gtagaccggg ccgccgccac attgttgttt tcttcagaag aagcacatcg
     5461 tctcagcggc gaggtcgttc ccatggacgc ctctcccggc ggcaaagggc gctggggcat
     5521 gacggtccgc gagccgctcg gcgtggtggc cgcgatcacg cccttcaact tcccgctgaa
     5581 ccttgccatg cacaagatcg gaccggcgct cgcggccggg aatgccgtcg tccataaacc
     5641 cgcatccacc acgcccatca gcgggttgag gatggccgga atttttgaga cctgtgggct
     5701 ccccaaaggc gcattgaacg tcatcaccgg acccggcgga acgattggag atctgttcac
     5761 gtttcacaag aacgtcgcga tgatcacgtt cacgggaagc acggacgtgg gacttcgtat
     5821 acgaaacctc gcgggattga agcgggtgac gctggagttg gggaacaact ccgccgtcat
     5881 cgtcgaagac gacgccaacc tggatgaggc cgtgccccgc tgcgttaccg gcagcttcgc
     5941 gcactcgggc caggtctgca tatccgtgca gcgcatcttc gtgcacgaga agattcggaa
     6001 ggaatttgtc gagcgctttg tagcggcggc cggcaagctg aagatcggtc atccgctgga
     6061 ccctgaaacc gacatcagct ccttgattac cgagaaggaa gcgcagcggg tggagagctg
     6121 ggtggacgaa gccgtaaagg cgggagcaac actggttcgt ggtggcagcc ggcgccgcgc
     6181 gaccatggaa cccgccgtcc tcaccgatgt gcctcccgat gcccagctat cgtgccgcga
     6241 agcgttcggc ccggtggtgg gcatcaatcc gtacacggac ctggacgaag ccatcgagca
     6301 ggtcaaccgc tccgaatacg ggcttcaggc cggcatctac acgcgggacc tcgaaaaagc
     6361 gtttcgtgcg gcccggcgcg tacacgtcgg cggtttcatg atcaacgaag tgccgcaata
     6421 ccgcgcggac cagatgccct atggaggcgt taaaatgagc ggaaccggcc gcgaaggccc
     6481 caaatacgcc atcgaggaga tgaccgaagt gaagctgatc tgctggaagc cctgacggcg
     6541 atcagaccga ttgaatggcc cgcatcaggt ccaccaggcc ggagccttcg aaataacgtt
     6601 cgcggccgag tgatgtggcg ctgttcagaa aaattctctt cacgtcttcg ggcttgccga
     6661 tgaactcccg cctgatcgaa agaaaggccg cgatcgcccc ggaaacatgc ggcgccgcca
     6721 tgctggtacc gctctcgtcg atataggcgg cagggtgttt gccttcttcg agcgagcata
     6781 ctccatcgag cagcttctgg cgtttcatca attcaagctt cttccctgtg gcgcaggaaa
     6841 caatacgctc gccgggagcg acaaggtccg gcttcagccg cccgtctccc gtcgggccct
     6901 tcgaagaaaa atacgatacg ccgtaagtat gagggctgtc cttgtgcgtg gagccaacag
     6961 tgatggcgag ggctgcgttt ccgggatcgt tgatggtcag cgagagaccg gtgttggtcg
     7021 ggcctgcctt cgccatcagc tcgccgtagc cggtatttcc cgccgcgatc acgacggcca
     7081 ccccactgcg aaccaggcgg tccacttcaa tgcacaccgg actctggccg caagcgaacc
     7141 acttggcgtt gaactcgtag cccaggctga tattcacacc gtgaacgcgc agcggcggtt
     7201 tgccatgccc gttcacatcg gagcggatat attcgagggc gcgcaggacg tctgtcgatc
     7261 gaacccgccc attgtcgtcc agcaccttga ggctgatgag tttggtgagc ggcgctacgc
     7321 ccgacaacag gccggggtgc cggactgtcc gccgttccag gcgttccagg tcggaatcgg
     7381 gcgacaccgc catccgctgg cccatgcaat attccacctt cgacccgtcc acctccgggc
     7441 ggcacaaggc gcccgcgata atccccgcga cgtgcgttcc gtgcccgaat ttatcggtca
     7501 gcgcaccctc tacatccggc tcatcttttg tgaaatcgcg atgcaggccg ctcacttccc
     7561 cacccagtgt ttcgtacaac tgaaaatgcg gatggtcggc ctgaattccc gagtcgatga
     7621 cggcccacac aatctcgctg cccgacgcat cgtaggcgcg ccgcgccgca tcggccttca
     7681 cggtcgcgac cgagcggtta ataaggggca tcaatcgaaa atccggccag atccggtaga
     7741 tacaacgccg cttggagtcc gtctcgctct gatcgcggct ggcgaggtcg cgcgcatcgc
     7801 tgatgctgag atagcagcgg taatacgtgt tcgcaatcag cagcggcggc ttcttctcgt
     7861 agcgggccgc gagggcatcg tgaatctgct ggaagcgttc gcgggagccc ggcaggcctt
     7921 tctcgtgaaa caggttcagc tccaccacaa ccgatgtggg cgtgaggtcg tcgggcggca
     7981 ttccgagctt gtcgcgttcg tgctggtctt cgatgtaaaa agcctcggat aagatgccgg
     8041 cccgaagcgg ttgctccccg tcgaatttct ctttcggagg cagcggtggc ggcgcgcctt
     8101 gatcgctcat ctattattaa ttttcctttc actcctcgcg gagtgccgct ggtcagtata
     8161 cacggcccgg gacatgcgga tttcgtgaaa tggtccagcg ttcactgccg aaggatcgga
     8221 gatgggtgag cattgggtga attcacgcat atcgtaattc ctggctacgc cggacctcat
     8281 tgaaaattat gcacttagcg aagcttttaa tgctggtaag caaattgcac ttttcagggc
     8341 atcatggcat acgtaattac cgacacgtgc accaaagacg agctttgcgt agacgcttgc
     8401 ccggtggact gcattcatcc gaagaaggac gacgccaaat tcgaggaagt gccgatgctc
     8461 tatgttaacc cagacgattg catcgactgc ggcgcctgcg ttcccgtttg tccgacctct
     8521 tccatctatg ttttggaaga attgccggag gacaaggccc acttcgccgc gatgaacgca
     8581 gcctactaca actaactcgc gaggggcagg cagccgggcg ccctgcccgc tcgtactgtc
     8641 cgcgccaaac ctggccaaca catcgggtgg atggatgatt tccgatcccg ctcttcgagg
     8701 gacctttgcc agtcgtaacc tcaacgctgg tggtaactgg aacctgacct atgacactct
     8761 cccggcactg gaagttgccg gaacggcact tcctgaacct tcgactttgt ctctgttcgg
     8821 ttccggagtc atcgggcttc tcctataccg ccgccgctcc tcttcgcgaa aggtttgatc
     8881 gcgctgactt agtgcagtgc gcacgtcgcg tcagaaatgt tcagactcta tacggttttt
     8941 ataacgctcg cttgctcgct gtgcgctcaa ccgaaccgct cgtatcggat agacacggtt
     9001 gcgggaaccg agccaaccga tgacggaacc gctgccaccg ccatccttaa ctctcctcgc
     9061 actcttgcca ttgcggcaga cggaacgatt tacgtcggaa gcgtagtcga aggcgtccgc
     9121 agcatcaccc ccggcggccg gataaccatg aatggcgccg gcgtcgtgat tgacatggaa
     9181 gtcgataagg acggtactct ctggtatctg actggcgggc ccgagctatg caaacaaact
     9241 gctaacggcg aacaaaagat attcaccccc aaccccactt ttggcccatc ccggttacca
     9301 attctaacga gcatagctct ggacccagac ggaaacctgg ttatcgccga tcaagagaac
     9361 cggagaatac tccggatgac ccctgcggga gtcgtctcgg tgatggcggg atcgggatcc
     9421 ctgacgcgtt tagacgcacc gcccaaccgt gtcgctgtcg atcggcagaa taacatctat
     9481 atagcgggtg agaaccggcg aatccttcgt ttgccggcaa acggcgcccc gacagtcata
     9541 gcgggaaacg ggcaattcgg agcaccggcg attggtagcc cggctacgtc gtccgcgttg
     9601 ggagccatca ccgctcttgc cgtcaacgac agcggagaaa tctttctggt tgacagctta
     9661 taccacgtga tcctgaaaat cggcacccaa ggaaatctgg aggccgtact ccgcgacgcg
     9721 gacgcttccg atcttgcatt cgacagcgcc gggcgcctgc tcttcacgga cactatcgcg
     9781 ggccgggtct ggcgcgtgaa tggcgacgga tcgcgaacct ccatcgccgg ccgcatccgc
     9841 ttcgaaggcg acggtggtcc agccatagat gcgttattga aatcgccgcg agcggttgcc
     9901 gttcatcccg atgggacgct tttcattggc gataccggga accatcgcat tcgcaaagtg
     9961 acacgggacg gtgtcatctc gacggtcgcc ggaaacggga cgccgggcgt cgcgggagat
    10021 ggcggacccg cggccgcagc gcaggtagcc gggccggatc atctggttat cgacggccgc
    10081 ggc
//
LOCUS       DASTMK010000014         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_96898, whole genome shotgun sequence.
ACCESSION   DASTMK010000014 DASTMK010000000
VERSION     DASTMK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96898"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFQ79_00510"
     CDS             complement(<1..930)
                     /locus_tag="VFQ79_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU0138154.1"
                     /translation="MFRKVLVANRGEIAVRIVRALREIGIRSVAVYSDVDRRALHVRM
                     ADEAAHIGPAPSTESYLRIDRILDAARKHGVDAVHPGYGFLSENADFAAACEEAGITF
                     IGPSSRSIRLLGTKTDARRLAIGVGVPIVPGTAAPVRTMEKAREAGRAIGYPVLLKAA
                     AGGGGKGMRRTGSDEEMVSAFRDASSEAERAFANAAVYVEKLVEPARHIEIQVLGDRH
                     GTMIHLGERECSIQRRHQKVIEEAPSPLMADHPGLRSRMGEAALKVARAAGYYNAGTA
                     EFLVDGHGNFYVLEMNTRLQVEHPVTELTTGHDL"
     gene            1029..1295
                     /locus_tag="VFQ79_00515"
     CDS             1029..1295
                     /locus_tag="VFQ79_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU0138155.1"
                     /translation="MTRLNVSKAREEFSEVVNRAAYGKERTVVSRRGKDLAAVIPIDD
                     LRLLERLAQKEIDRIDLEDARAALKEAEEKGTIPLKEAKKRLAL"
     gene            1463..1606
                     /locus_tag="VFQ79_00520"
     CDS             1463..1606
                     /locus_tag="VFQ79_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0138156.1"
                     /translation="METKEKLYRVYVGPGKNYRAIYQIRDEVLLVLVVKVGDRKEVYR
                     RVK"
     gene            1738..2463
                     /gene="tsaA"
                     /locus_tag="VFQ79_00525"
     CDS             1738..2463
                     /gene="tsaA"
                     /locus_tag="VFQ79_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEU0138157.1"
                     /translation="MIALEPIGVVRSEIGEGQPMPPLGVPAVIEIFPRFLDGLHRIEK
                     HSHIWVLAWLDNAERDLLQVKPRGLKDAGDEGLHGVFAVRSPARPNPVGLTATRVLEI
                     KHLSIHVERLDFRDGTPVVDVKPYFISRDMIFSASNVQIGKPLTPEAAREALLLQAVH
                     FHGELCDDLDRAVNVMARFRAEVLEWTDPAGWAVTVPADRPCIADAFMGMLRVSPGRK
                     NLVFGAPGKISFERGADRYSYDI"
     gene            complement(2480..3670)
                     /locus_tag="VFQ79_00530"
     CDS             complement(2480..3670)
                     /locus_tag="VFQ79_00530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0138158.1"
                     /translation="MPDRPKTLVLAPEVPIPVIGGGALRTASVLHYLACRSDVDLIVF
                     QQPGAVHPAERVPAGLIRRLHVINLPFHSRGRFSRTARNLKRLVSGTPPLIDRFAGFE
                     SRVAAFAGLEKYEIGLVEHFWCAPYQRVLAPRCNTTVLDLHNIESLWHERCGAVEQPP
                     VRLAFQRFAKACRRLEREWLPRYSTVLTVSENDAAAAAELSSGANIAVYPNTIPWEAV
                     PSKEKMHAVVFTGNIEYRPNKTGLDWFCREIWPQVRELAPHAEVWIAGMNAGRARTLW
                     AGVPGLRIFSDYENAVGLIASAKVAVVPIRAASGSRFKILEAWAAGVPVVSTTIGAEG
                     LSAEHGRNIVIADDPEPFARAVSRLLSDDELRALIGAEGRSLFEAEYTWEHGWAHLDR
                     ILQL"
     gene            complement(3663..>4541)
                     /locus_tag="VFQ79_00535"
     CDS             complement(3663..>4541)
                     /locus_tag="VFQ79_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0138159.1"
                     /translation="GPRNALESKWWTIGLARELSKLGTDVFHGTDFAVPYIPLRPSVM
                     TVHDLSPWIGPGKEDASIRVQRRTPILAGLGLATMIITPTVSVRRQVLDHFQVSPHRV
                     AVTPLAASSRFRPNGARRGRFFLLPGTRQARKNIALLLQAWRELRKSEEVDLVLAGRS
                     RETESGHEIPGLHILGEVSEEKLISLYSEALAVIYPSVYEGFGLPVLEAMQCGAAVIT
                     SKDPAVGEVAGDAAVRIDAGDVRGWVEAMRKAATDADWASGLRERALLRARDFSWQRT
                     ARATREIYHEARCRFA"
BASE COUNT          940 a         1418 c         1334 g          849 t
ORIGIN      
        1 caggtcgtgg ccggtcgtca actctgtcac gggatgctcc acttgcagcc gtgtattcat
       61 ctcgaggacg tagaagttgc catggccgtc caccagaaac tcggcggtac cggcgttgta
      121 gtagccggcc gcgcgcgcca ctttcaaggc ggcctctccc attcgcgaac gaagaccggg
      181 atgatcggcc atgagcggag acggagcctc ttcaatcacc ttctgatgac ggcgctggat
      241 cgagcactcg cgctctccca ggtgaatcat tgtgccatgc cggtctccga gcacctgaat
      301 ctcgatatga cgggccggct ccaccagctt ttccacatag acggcggcat tcgcgaaagc
      361 gcgctcggct tcgctggagg cgtcacgaaa cgcggagacc atctcctcgt cggagccggt
      421 ccggcgcatt cctttgcccc cacccccggc ggccgctttc agcagaaccg gatagccaat
      481 tgcgcggcct gcctcgcgcg ccttctccat ggtgcgaacg ggcgccgcgg tgccgggcac
      541 gatcggcaca cctacgccga tcgccaggcg gcgggcatcc gttttcgttc ccaacaggcg
      601 gatggacctg gaagacgggc cgataaacgt gatacccgcc tcctcacacg cggcggcgaa
      661 atccgcgttc tcgctcagaa agccatagcc ggggtgaacg gcgtcaacgc cgtgtttgcg
      721 cgccgcatcc aggatgcggt cgattcgcaa atagctttca gtcgaagggg ccgggccgat
      781 atgcgcggcc tcatcggcca tgcgcacgtg aagcgcccgg cggtccacgt cggagtaaac
      841 agcgacactc cggataccta tttcgcgcaa cgcgcgcacg attcgcaccg caatctcgcc
      901 gcggttcgcc accagaacct tgcggaacat gaatcgagat tgtagcagga tcgcagcctt
      961 gcaaagcccg ctattctgcc cgatactgaa tgtagaatcg tacaacttgt acgagtggta
     1021 cacacaccat gacacgcctg aatgtctcga aagcgcggga ggaattttct gaagtcgtaa
     1081 accgcgccgc ctacggaaag gagcggaccg ttgtttcccg ccgtgggaag gacttggccg
     1141 ccgtcatccc gattgatgac ctccgtctcc tggagcgcct ggcccagaag gaaatagacc
     1201 ggatcgacct tgaagacgct cgggccgcct taaaagaggc ggaagagaag gggaccattc
     1261 cacttaaaga agcgaagaaa cgcctggcct tgtaggcaca tgcctagacg aacaaccggg
     1321 tgatgactga accgaagaag ccgtcatacg ccctggagtt tactcacgcc gctatccggg
     1381 aacttgagaa gctggacccg aatatccgcc ggaaggtctt ttccgacatc gaaaaaaccc
     1441 tcgcccctat ggcgtggaga agatggaaac aaaggagaag ctctaccgcg tctatgtggg
     1501 accagggaag aactaccgag ccatctatca gattcgggat gaagttcttc tcgttctcgt
     1561 agtgaaggtg ggagaccgga aggaagtcta ccgtcgcgtg aagtgaagcc catatggctt
     1621 ccctcgttga aacaccgggg gccgtccggg cgacacgatc cctggaatcg ataagatagt
     1681 gttgcattca cacatggaag tctcgcatct atacatctct catcatggtt tgcggccgtg
     1741 atcgcgctcg aacctatcgg cgtagtgcgc tcggaaatcg gcgaagggca gccaatgccg
     1801 ccgctcggag tcccagcggt gatcgaaatt tttccccggt tcctggatgg tctgcatcgc
     1861 attgaaaaac actcgcacat ctgggtgctc gcctggctgg acaacgcgga acgggacctg
     1921 ctgcaagtga agccgcgcgg tttgaaggac gcgggcgacg aagggctgca cggagtgttt
     1981 gccgttcgct ccccggcgcg cccgaatccc gtgggcctca ccgctacacg tgttctcgag
     2041 atcaaacatc tgtcgatcca tgtcgagcgg ctcgacttcc gggatggaac tcctgtcgtg
     2101 gatgtgaagc cgtatttcat cagccgggat atgattttct ccgcctcaaa cgtgcaaatc
     2161 ggaaaaccgc tgactcccga agcggcgcgc gaggcgctgc tccttcaggc ggtccatttc
     2221 cacggggagt tatgcgacga tctcgaccgg gccgtgaacg tgatggctcg tttccgcgcg
     2281 gaggtgctgg aatggacgga cccggcaggt tgggccgtca ccgttcccgc ggaccggcca
     2341 tgcatcgccg atgccttcat gggcatgttg cgagtgagcc cgggccggaa aaatctcgta
     2401 ttcggcgcgc ccggtaaaat cagcttcgaa cgcggcgccg atcgatacag ctacgacatt
     2461 tgatgcgttc ccgtggcgct cacaactgca gtatgcggtc gagatgcgcc caaccatgct
     2521 cccacgtata ttccgcttcg aagagggaac ggccttcggc gcctataaga gcccgaagct
     2581 cgtcgtcgct caacagccgg gacacggccc gcgcgaaggg ctccggatcg tcggcgatga
     2641 cgatgttccg gccgtgctcg gcgctcagcc cttccgcgcc gatcgtggtg gagaccacgg
     2701 gaacgcccgc cgcccaggct tccagtatct taaagcgcga gccgctggcc gcacggatcg
     2761 gaaccacggc cactttcgcc gatgcgatca ggccgaccgc attttcgtaa tcggagaaga
     2821 tccgcaagcc ggggacgccc gcccaaagag tgcgggcccg gccggcgttc atgcccgcta
     2881 tccaaacttc ggcgtgcggc gcaagctccc gcacctgcgg ccagatttcg cggcaaaacc
     2941 aatccagacc agttttgttg ggccggtact cgatatttcc ggtaaaaacc acagcgtgca
     3001 ttttttcctt cgacggcacg gcctcccatg gaatcgtgtt cggatacacg gcaatattgg
     3061 cgccggatga gagttcggct gccgccgccg catcattctc cgaaaccgtc agcacggtag
     3121 agtaacgcgg cagccattcc cgttccaggc gccggcacgc tttagcgaat cgttgaaatg
     3181 ccaggcgcac cggaggctgc tcgaccgcgc cgcatcgctc atgccacagt gactcgatat
     3241 tgtgaagatc caacaccgtc gtgttgcagc gcggagccag aactctctgg taaggcgcgc
     3301 accagaaatg ttccaccaat ccgatttcgt acttttcgag accggcgaac gcagccaccc
     3361 tgctctcaaa accagcgaat cggtctatca gcggcggcgt acccgatacc agcctcttca
     3421 ggtttctggc ggtccttgaa aaacgtcctc gcgagtggaa tgggaggttg attacgtgca
     3481 gcctccggat gagtccggcc ggtacacgct ccgccggatg gaccgcgccg ggctgttgga
     3541 atacgattag atccacatcc gagcggcacg ccagataatg cagaacgctc gcggtgcgca
     3601 gtgcgcctcc accgattacc ggaatgggta cctccggcgc caggacaagg gtcttgggcc
     3661 tgtcaggcaa agcggcaccg ggcttcgtgg tagatttctc ttgtcgcgcg cgcggttcgc
     3721 tgccacgaaa aatcacgggc ccgcagcagt gcgcgctccc gtaagccgga agcccaatcc
     3781 gcatccgttg cggctttccg catggcttcc acccagcctc tcacgtcgcc tgcgtcaata
     3841 cggacggccg catcgcccgc cacttctcca acggccgggt cttttgacgt gatgaccgcc
     3901 gctccacact gcatcgcttc cagcacaggc aggccgaatc cctcataaac ggaggggtaa
     3961 atcaccgcga gagcctcgct ataaagtgaa atgagtttct cttcggagac ctctccgaga
     4021 atatggagtc ccgggatttc atgaccggac tccgtttcgc ggctgcgccc cgccaggaca
     4081 agatccactt cctcggactt gcgcaactcc cgccacgcct gaagaagcag ggctatgttt
     4141 ttgcgagcct gacgggtccc gggcaaaaga aaaaaccgtc cgcggcgcgc accattcgga
     4201 cggaaccggc tcgaagctgc aaggggtgtc acggccaccc tatgaggcga aacctgaaag
     4261 tgatccagca cctgcctgcg gacggagacc gtgggagtaa taatcatcgt cgccagacca
     4321 agccccgcca ggatgggagt acgcctctgt actcgtatgg aagcgtcttc tttcccggga
     4381 cctatccagg gagaaaggtc gtgaacggtc attacgctgg gcctcagggg aatgtaaggc
     4441 acagcgaagt cggttccatg aaagacatcg gtcccgagtt tcgacagctc ccgcgcaagc
     4501 ccgatggtcc accactttga ttcgagagcg tttcgcggac c
//
LOCUS       DASTMK010000015         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_65159, whole genome shotgun sequence.
ACCESSION   DASTMK010000015 DASTMK010000000
VERSION     DASTMK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65159"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFQ79_00540"
     CDS             complement(<1..362)
                     /locus_tag="VFQ79_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU0138160.1"
                     /translation="MTLINRAESYFEFAKLGTDWRTEILAGFTTFITMAYIIFVNPSV
                     LKEAGMPFTAVVAATCLSAGLGSILMGIFARYPIALAPGMGLNAYFTYSVVKGMGVPW
                     EVALGAVFLSGVAFVLLT"
     gene            complement(393..1286)
                     /locus_tag="VFQ79_00545"
     CDS             complement(393..1286)
                     /locus_tag="VFQ79_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU0138161.1"
                     /translation="MWRRLYYCDHHEMGLPPGHKFPARKYRLLRELLEQENSFEFVAA
                     EPAPLDAITRVHDRGYVESFVNGSIPYAAMRRIGFPWSESLVRRTLGSVGGTLAAAMD
                     ALESGWGGNLAGGTHHACRSDGAGFCVFNDIAVAVLSLRECHAIRRVAILDLDVHQGD
                     GTARIFEDDDMVLTVSVHNRNNFPFRKQPGKIDVDLPDGTGDSEYLDAVDKVLARVLE
                     FKPEMLYYQSGVDPLAEDRLGRMSLTQAGLAGRDRWVFAASKAAGIPVAVTLGGGYAE
                     PIELTAEAHANTFRCALEVFG"
     gene            complement(1290..1922)
                     /locus_tag="VFQ79_00550"
     CDS             complement(1290..1922)
                     /locus_tag="VFQ79_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0138162.1"
                     /translation="MKKMIVLFLFAAAAFAQRTPRPVLPGEDWVQLFNGKDLSGWVEI
                     GKEKWTVENGVIRGQAISKAYGYLQTEKSYKDFELALQFKCQGSGNSGVFFHVGFKPG
                     TPNVSQGLQFEIDCALGKHTGGVYGDGRQWIVWPAPENEIVVRRGEWNEYLLKVQGNR
                     YTSRLNGVQMIDFTDPQPKSFDGPIALQLHSGGEGDMTFREIYIRDLSKR"
     gene            complement(1952..>3193)
                     /gene="glmM"
                     /locus_tag="VFQ79_00555"
     CDS             complement(1952..>3193)
                     /gene="glmM"
                     /locus_tag="VFQ79_00555"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU0138163.1"
                     /translation="AAADPEVVIGMDTRESGPWLAGQVAGGLERYGVRTRFAGLITTP
                     GVAYLTRTQSFVAGVMISASHNPYQDNGIKVFDHSGFKLPDEVEHALEEDIFRLRAGD
                     AEPVPRDLLVDEALDETYIDFLASVAGVSLAGLTIVLDCANGAASHLAPELFERLGAH
                     VYSLGCLPNGRNINLNCGALHVESLRGSVVEKGADIGIAFDGDADRAIFVTSTGKIAS
                     GDHVLMMCGKDLLRRGKLSSKEGEPLVVATIMSNLGLERALAAAGIRLVRTAVGDKYV
                     LEEMLARGAMLGGEQSGHVIFREHATTGDGLLTALQVLQVMRNSGRSLDELTGELEIY
                     PQVLVNVRVKTKRPLKELSPVNEEIRAAEAEFGEAGRVVVRFSGTEPLARVMVEGPEE
                     KRVQQAAERIASVIRSELGGV"
BASE COUNT          629 a         1066 c          879 g          619 t
ORIGIN      
        1 aacgtcagca gcacaaacgc aacgccggac agaaacacgg ccccgagcgc cacctcccac
       61 ggcacaccca ttcctttcac caccgaatag gtgaagtaag cgttcaatcc catgcccggc
      121 gcaagtgcga taggatagcg cgcgaagatt cccatcagga tactgccgag ccccgcggat
      181 aaacaggtag cggccaccac cgccgtgaaa ggcattcccg cctctttgag tacggacggg
      241 ttcacgaaga tgatgtaagc catcgtgatg aacgtggtga acccggcgag aatctcggtg
      301 cgccagtccg ttcccagctt cgcgaactca aagtacgatt cggctctgtt gataagggtc
      361 atcggacttc cccagacttg ggcggcggcg cttcagccaa aaacttcgag cgcgcaccgg
      421 aaggtgttgg catgggcttc cgccgtcaat tcaatgggct cagcgtagcc gccgcccaga
      481 gtaacggcca ctggaatacc cgctgccttc gaggcagcaa atacccagcg atccctcccg
      541 gcgaggcctg cctgtgtcag cgacatccgc cccagccggt cttcggccag agggtccact
      601 ccggactggt agtacagcat ctcgggtttg aattcgagca cgcgggccag caccttatcg
      661 acggcgtcca gatactcgct gtctccggtc ccgtccggca gatcgacatc gatcttgccc
      721 ggctgtttgc ggaaagggaa gttgttccgg ttgtgaacgg agacggtcag caccatgtcg
      781 tcgtcctcga aaatccgggc agttccgtcg ccctggtgaa catcgaggtc gagaatcgcc
      841 acgcgccgta tggcatggca ttcccgcagc gatagtacgg cgacggcgat atcgttgaac
      901 acgcaaaatc ccgcgccgtc cgaccggcaa gcgtggtgtg tgccgccggc gaggttgcct
      961 ccccatccgg attcgagagc atccatcgcg gcggcgagag taccgcccac ggaacccaag
     1021 gtgcgccgga ccaggctttc ggaccacgga aagccgatcc ggcgcatggc agcgtaagga
     1081 atcgacccgt tcacgaaaga ttcgacatag ccgcgatcat gcacccgcgt aatcgcatcc
     1141 agcggcgccg gctccgccgc aacgaattcg aagctgttct cctgttcgag caactcccgc
     1201 agcagccggt atttccgcgc tggaaatttg tggcccggtg ggagccccat ctcgtgatgg
     1261 tcgcagtagt acagcctgcg ccacatgcct cagcgtttgc tcagatcgcg tatgtaaatt
     1321 tcccggaacg tcatgtcgcc ctcgccgccc gagtgcaact gaagcgcgat gggaccatcg
     1381 aacgatttgg gctgcgggtc ggtgaaatcg atcatctgta cgccgttcag gcgggaggtg
     1441 tagcggttcc cctgcacttt gagcagatac tcgttccatt cgccgcggcg caccacgatc
     1501 tcgttttccg gcgcgggcca gacaatccac tgcctgccat cgccatagac gccgcccgtg
     1561 tgcttgccca gagcgcagtc gatttcaaac tgcagcccct ggctcacatt gggtgtgccc
     1621 ggcttgaaac cgacgtgaaa aaagacgccg gagtttccgc tgccctgaca cttgaactgc
     1681 aatgccagct caaaatcctt gtagcttttc tccgtctgga gatacccata agccttgctg
     1741 atggcctggc cgcggattac tccgttttcg acggtccact tttctttacc gatctcaacc
     1801 cagcccgaga gatctttacc gttgaacagt tggacccaat cctctccagg caacaccggc
     1861 cgcggcgttc tttgcgcaaa ggcggcggcc gcgaacagaa acagaacgat catctttttc
     1921 atggctgatc cagtccttat tgaaaaagac atcatacccc acccagttcg cttcgtatca
     1981 cactcgcgat acgctcggcc gcctgctgta cgcgtttctc ctcgggaccc tccaccatca
     2041 ctctcgccag cggctccgtt ccggaaaatc gcaccacgac gcgcccggct tctccgaatt
     2101 ccgcctccgc cgcccggatc tcctcgttta ccggtgaaag ctccttcagc gggcgcttcg
     2161 tcttcacgcg tacgttcacc agcacctgcg ggtaaatttc caattccccg gtgagttcgt
     2221 ccaggctgcg gcccgaattc cgcatgacct gaagcacctg aagcgcggtc agaagcccgt
     2281 ctcccgtcgt tgcgtgttcg cggaatatga cgtgccccga ttgctcaccg cccagcatcg
     2341 cgcctcttgc gagcatctct tcaaggacat acttgtcgcc caccgctgtt ctcacaagcc
     2401 gtatgccggc cgccgcaagc gcgcgttcca ggccgaggtt cgacatgatc gtggccacca
     2461 ccagcggctc gccttctttg ctcgagagtt tgccgcggcg aagcaggtct tttccgcaca
     2521 tcatcagcac gtggtcgccg ctggcgatct ttccggtgga ggtgacgaag atcgcacggt
     2581 ccgcgtcgcc gtcgaaagcg attcctatgt ccgctccctt ttccaccaca ctgccgcgga
     2641 gcgattccac gtggagcgcc ccgcaattca gattgatgtt cctgccattg ggaaggcagc
     2701 ccaacgagta cacatgcgcg cccagccgtt cgaacagctc cggcgccagg tgcgaagccg
     2761 ctccattcgc gcagtcgagc acgatcgtga gcccggccag cgatactccc gcgacggacg
     2821 ccaggaagtc gatatacgtc tcatccaacg cttcatccac cagcaggtcc cgcggaacgg
     2881 gttcggcgtc ccccgcgcgc aggcggaaaa tatcttcctc cagcgcatgc tccacctcgt
     2941 cgggaagctt gaagccggaa tggtcgaaca ccttgatgcc gttgtcctgg taaggattgt
     3001 gggaagcgga aatcattaca cccgccacga acgactgggt gcgggtgaga taagctacgc
     3061 ccggcgtggt gatcagtccg gcaaaccggg tacgaacacc gtacctttcg agcccgcccg
     3121 ccacctgccc ggcgagccat ggccccgatt cgcgcgtgtc catgccgatc acaacctccg
     3181 gatcggctgc cgc
//
LOCUS       DASTMK010000016         5391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_545020, whole genome shotgun sequence.
ACCESSION   DASTMK010000016 DASTMK010000000
VERSION     DASTMK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545020"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VFQ79_00560"
     CDS             complement(<1..165)
                     /locus_tag="VFQ79_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0138164.1"
                     /translation="MPLPDSSVDVIISNCVINLSADKDRVLREAFRVLKPGVRFAVSD
                     VVTRGGIPEEI"
     gene            complement(149..577)
                     /locus_tag="VFQ79_00565"
     CDS             complement(149..577)
                     /locus_tag="VFQ79_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138165.1"
                     /translation="MPLAYRSERDEINGPTFRRWRTNACCGSSPSSCCSGGDPISSDL
                     YNQLQKDELPETAVLASLGCGNPTALAGLKPGEVVLDLGSGGGIDVLLSAKRVSPGGK
                     AYGVDMTDEMLDTRPKQLEEGRSGKRKVSERRDLANPSAR"
     gene            598..1806
                     /locus_tag="VFQ79_00570"
     CDS             598..1806
                     /locus_tag="VFQ79_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138166.1"
                     /translation="MKLSRRSLFAVGLGSALTACSRSKEAENTEASAGNKLPLEEFQP
                     KSMLVTPQNPKPSAKYPVIDVHTHLGAVFGRERTPGPNGPSPEAVQQLDQILGWMNEL
                     NMKTLVNLTGGYGDALKNNITTLSRGKYEGRFVNCVVPAWDRIREPDYPRWQAEELSR
                     ARDAGAIGLKISKTLGLYLRENGKEGPLVKIDDSRFDPMWEAAGKLKLPVMIHIADPD
                     AFFTTIDRFNERWEELANHPNWSFYGKDFPPKQELLDARNRVIERHPGTTFVGLHVAN
                     HPENLDDVSAWLKKYPNMHCEIGARVGELGRQPRRARRFFEEFQDRIMFGTDATPNGT
                     NVPQQDLKPAMFQCYFRFLETADEYFDYAPSEVPPQGRWKIYGIELPDSILKKVYHNN
                     AARLLGWKTI"
     gene            1803..3137
                     /locus_tag="VFQ79_00575"
     CDS             1803..3137
                     /locus_tag="VFQ79_00575"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138167.1"
                     /translation="MTELMDLDRRDLLKTGLGVTAAAFSPLAAQQKESIRIGFVGVGG
                     RGTGLLRNLLKLEGVKVNALCDINEANLARAQKLVADAGQPKPQGYSKSETDWRNLCE
                     RKDLDIVFNATPWQWHTPIAVAAMKAGKDTATEVPAALTVEECWELVETSEKTGRHCA
                     MLENDCYYREVLMILTMIRAGALGEPLMAEAGYLHDLRTVKFNTVPHGEPWRLDHTIK
                     RNGNLYPTHPMGPMAWWLDINRGDRMTYLVSMSSKHAAMKEFAIREFDPNDWRSKTDY
                     ALGDVNVTLIRTEEGKTITLWFNTNSPRVKEHLLRIQGTKGIFNSMMDRVFVEGRSNR
                     GEGENWRARHDWEETAEYRKQYDSKLWKNMEEQARGAGHSGVDYMQLYRLIKNLREGR
                     PPDIDVYDAAAWSVLSPLSEASVAGRSKPVDIPDFTRGKWKLRAPVDPDAIV"
     gene            3152..4441
                     /locus_tag="VFQ79_00580"
     CDS             3152..4441
                     /locus_tag="VFQ79_00580"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138168.1"
                     /translation="MPMANRRDFFSAMLQAAGVRDTKPIRAGIVGVGDRGSYHMDLLL
                     GTDLVEIPAICDINPEYLYRAKRWIEQAGRPSPELYDRGKTDFERLCERKDLDLVVCA
                     TSWEWHAPVCIAAMKAGKHATTEVPAALTVDECWEMVEVSEKTRKHCVMLEQANYSRD
                     GLMVLEMAQKGAFGQILASTGGYVHDLRLVKFDPEREPWRLQHSVDRNGNLYPTHPIG
                     PMAWWMDINRGDKFEYLVSMSSKAVCLNEYASNYFGDRHPYAGMKMNQGDINTSLLRT
                     ANGKLAVLYYDTNTPHPQTAELRLDGSKGHYAGNIQQVFIEGRSPKAHAWEPLANYKS
                     EFDPPIWKNLDPGKFKRARGHGGGETTPLMWARLLRALQRGTEPDMNVYDAVVWSVIS
                     PLSERSVAQGSRPTDFPDFTRGKWKNTPPIRLEPESA"
     gene            4462..5391
                     /locus_tag="VFQ79_00585"
     CDS             4462..5391
                     /locus_tag="VFQ79_00585"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU0138169.1"
                     /translation="MPKRLAISLCGIPLKNPVIAASGTFGYGVDFEKILDLNAVGGLV
                     VKGLSREPIEGNPPPRLWESEGGMINSVGLQNVGVRAFVLEKLPRLRRFDTAVFANVF
                     GYAPEDYVEVVRMLEDAEGLVGYELNVSCPNTKHGGIFFSSDPALLASLVASVKRAAR
                     RPVIVKLSPNVASIEPLAKAAEEAGADALSLVNTFVALAVDARTRTPRIGAGFGGLSG
                     PAIKPIALRMVFQAARAVRIPVIGLGGIASGEDAAEFLIAGASAVQVGTASFWDPQAV
                     LRIAAELGAFLESQNIANVTELVGTLKFSGGCG"
BASE COUNT         1163 a         1582 c         1621 g         1025 t
ORIGIN      
        1 gatttcctcg ggaatgccgc ctcgcgtcac cacatcggag accgcgaacc gtacgccggg
       61 cttaagtacc cggaacgctt ccctaaggac gcggtccttg tcggctgaaa gattgattac
      121 gcaattggag atgatgacgt cgacggaact atcgggcaga gggatttgct aaatctcgcc
      181 tttcagaaac tttacgtttt ccactccggc cttcttctaa ttgtttcggg cgagtgtcaa
      241 gcatctcatc ggtcatgtcg accccgtagg ctttgccgcc aggagagact cgctttgccg
      301 aaagcaatac gtcgatgccg ccgccacttc ccaggtccag aacgacttcg cctggtttca
      361 gtccggccag cgccgttggg tttccgcagc cgagcgacgc cagcactgcc gtttccggca
      421 gttcgtcctt ttgaagctga ttgtagagat ccgaactgat cggatctcct cctgagcagc
      481 aggatgacgg gctgctgccg cagcaggcgt tcgttcgcca gcggcgaaac gtagggccgt
      541 tgatttcatc ccgttcggaa cgatacgcta agggcaagtc gaataggacc gaattatatg
      601 aaactttcgc gccgctctct tttcgcggtg gggctcggtt ctgcccttac cgcctgctcc
      661 cggtccaagg aagctgaaaa caccgaagca tccgccggca acaagctccc tctcgaggaa
      721 ttccagccaa agagcatgct tgttacgccg cagaacccga agcccagcgc caagtaccct
      781 gtcatcgacg ttcacacgca tctcggcgcg gttttcggac gcgaacgcac acccggtccc
      841 aacggcccca gccccgaggc cgtgcagcag ctcgatcaga tcttaggctg gatgaacgag
      901 ctgaatatga agacgctggt caacctcacc ggcggttatg gagatgccct gaaaaacaac
      961 atcaccacgc tgtcgcgcgg caagtatgaa ggacgcttcg taaattgcgt ggttccggcg
     1021 tgggaccgga tcagggaacc ggactatcct cgctggcagg cggaggagct ttcgcgcgcc
     1081 cgggacgccg gcgccatcgg cctgaagatc tccaaaaccc tcggcctcta tctgcgcgag
     1141 aacggcaaag aagggccgct cgtcaaaatc gacgattccc gcttcgatcc gatgtgggaa
     1201 gccgccggga agctgaagct gccggtaatg atacacatcg ccgatccgga cgcgttcttc
     1261 accaccatcg accggttcaa cgaacgttgg gaagagttgg ccaatcatcc caactggtct
     1321 ttctacggca aggattttcc gccgaaacag gaactgctgg acgcgcgcaa ccgcgtcatc
     1381 gagcggcacc cgggcacgac cttcgtcgga ttgcacgtgg caaatcaccc ggaaaacctc
     1441 gatgacgttt ccgcctggct gaagaagtat cccaacatgc attgcgagat cggcgcccgc
     1501 gtcggtgaat tgggccggca gccccgccgt gcgcgccggt tcttcgaaga gtttcaggac
     1561 cgcatcatgt tcggcaccga tgcaacgccg aatggaacca atgttcctca gcaggacctg
     1621 aagcctgcca tgtttcaatg ctatttccgg ttcctcgaaa ccgcggacga gtacttcgat
     1681 tacgctccga gcgaagtgcc gccgcaaggc cgctggaaaa tctacggcat cgagctgcct
     1741 gactcgatcc tcaagaaggt gtaccacaat aacgccgcca ggcttctggg atggaaaacg
     1801 atatgaccga acttatggat ctggacaggc gggacctgct gaagacaggt ctcggcgtaa
     1861 cggcggcggc cttctcaccg ctggccgccc aacagaaaga gtcaatccgc atcggctttg
     1921 tgggcgtcgg cggacgcggc accggtttac tgcggaacct gctgaagctg gagggtgtaa
     1981 aagtcaacgc cctttgcgac atcaatgaag caaacctggc tcgcgcacaa aaactggtgg
     2041 cggatgccgg ccaaccgaag ccgcagggtt attcgaaaag cgagaccgac tggcgcaatc
     2101 tctgcgagcg caaagatctg gatatcgtgt tcaacgccac cccctggcag tggcatacgc
     2161 ccattgcggt ggccgctatg aaggccggaa aggacactgc caccgaagtg cccgccgcgc
     2221 ttaccgtgga agagtgttgg gagctggtcg agacttcgga aaaaaccggc cggcactgcg
     2281 ccatgctgga gaacgactgc tattaccgcg aagttctcat gatcctcacc atgattcgcg
     2341 ccggtgcgct gggtgagccg ctgatggccg aagccggata cctgcacgat ctccgcacgg
     2401 taaagttcaa caccgtgccg cacggggagc catggcgcct ggaccatacc atcaagcgca
     2461 acggcaacct gtacccgaca cacccgatgg gtcccatggc gtggtggctc gatatcaatc
     2521 gcggcgaccg gatgacttac ctggtctcga tgagttccaa gcacgcagcg atgaaggagt
     2581 tcgccatccg cgaattcgac cccaacgact ggcgctccaa aaccgattac gcgctgggcg
     2641 atgtcaatgt tactttgatc cggaccgaag aaggaaaaac gatcacgctc tggttcaata
     2701 ccaacagccc ccgcgtgaag gaacacctgt tgagaattca gggcaccaag ggcatcttca
     2761 acagcatgat ggatcgcgtc ttcgtggaag gccgcagcaa ccggggcgag ggcgaaaact
     2821 ggagagcccg gcacgattgg gaagagacgg cggaataccg caagcagtac gactcgaagc
     2881 tgtggaagaa catggaggaa caggcgcggg gagccggcca tagcggcgtg gactatatgc
     2941 agttgtaccg gctcattaag aacctgcgcg agggcaggcc gccggacatc gacgtctacg
     3001 acgcagcagc ctggagcgtg ctttcgccgc tctcagaggc atccgtcgcc ggccgtagta
     3061 aacccgtgga catccccgac ttcacccggg gtaaatggaa actgcgtgcg ccggtggacc
     3121 ccgacgcgat tgtgtaggag gagatcggat catgcccatg gcaaatcgaa gagacttttt
     3181 cagcgcgatg ctgcaggccg ccggcgtgcg tgatacgaag cccattcgcg cgggcatcgt
     3241 cggcgtggga gaccgcggtt cttaccacat ggatcttctg ctgggaaccg atctcgttga
     3301 gatcccggcc atctgcgata tcaatcccga atacctgtat cgggcgaagc gatggatcga
     3361 gcaggccggc aggccttcgc cggaactcta tgatcgcggc aagaccgatt tcgagaggct
     3421 ttgcgaacgg aaagacctgg acctggtcgt ctgcgccacc tcgtgggaat ggcatgcacc
     3481 cgtctgtatc gctgcgatga aagcaggcaa gcatgccacc acggaagtac ctgccgccct
     3541 cacggtggat gaatgctggg agatggtgga ggtttccgaa aagacgcgta agcattgcgt
     3601 catgctggag caggccaatt acagccgcga cggtctgatg gttctcgaaa tggcgcaaaa
     3661 gggcgcgttc ggccagatat tggcctcgac gggcggatac gtacacgacc tccggctggt
     3721 gaaattcgac cccgaacgcg agccgtggcg gttgcagcat tccgtcgacc gcaatggcaa
     3781 cctgtatccg acgcatccca tcggtcccat ggcctggtgg atggacatta accggggaga
     3841 caagtttgaa tacctcgtgt cgatgagctc gaaagccgtt tgccttaatg agtacgcctc
     3901 gaactacttc ggcgaccgcc acccgtacgc gggaatgaaa atgaaccagg gcgacataaa
     3961 tacttcgctt ctgaggacgg cgaacgggaa gcttgcggtg ctctattacg acaccaacac
     4021 gccgcacccg caaaccgctg agctgcgtct ggacggcagc aagggacact acgccggcaa
     4081 catccagcag gtcttcatcg aagggcgcag cccgaaagct catgcatggg agccgcttgc
     4141 caactataag tccgaattcg atccgccgat ctggaagaac ctggatccgg gtaagtttaa
     4201 gcgcgctcgc gggcacggtg gtggcgaaac cacgccgctg atgtgggcca ggttactgcg
     4261 ggcattgcag cgaggcaccg aacccgatat gaacgtctac gacgcggtcg tgtggagcgt
     4321 catctcacct cttagcgagc gctccgtcgc gcaggggagc cggcctacgg acttccccga
     4381 tttcacgcgg ggtaagtgga agaatacgcc tcccatccgg ctggaacccg aaagtgcctg
     4441 agcattgtag actgaaagct ggtgcccaaa cgcctcgcga tttctttgtg tggcatacct
     4501 ctgaagaacc ccgttattgc tgcttccggt accttcggat acggcgttga tttcgaaaag
     4561 attctagacc tgaacgccgt tggcgggtta gtcgtcaagg gactttcgcg cgagccgatc
     4621 gaaggtaatc cgccgccgcg cctttgggaa tccgaaggcg gcatgatcaa ctccgtgggc
     4681 cttcagaacg ttggagtgcg cgcatttgtg ttggagaaac tcccgcgtct caggcgattc
     4741 gacaccgcgg tattcgccaa cgtgttcggc tacgctcccg aggattatgt ggaggtcgtc
     4801 cggatgctgg aggacgcgga aggcttggtg ggctacgaac taaacgtctc ctgcccgaac
     4861 acgaaacatg gcggcatctt cttctcgagt gatccggcgc tgctggcgtc gctggtcgcc
     4921 tctgtgaagc gagctgctcg cagaccggtg atcgtgaagc tttcgccgaa tgtcgcgagt
     4981 atcgagccgc tcgccaaagc ggcggaagag gccggcgcgg acgctctatc gctggtgaac
     5041 acctttgtgg cactcgcggt ggatgctcga acaaggactc cacgcatcgg tgcggggttc
     5101 ggcgggctct ccggaccggc catcaaaccc atcgcgttgc gcatggtctt ccaggcggcg
     5161 cgcgcggtcc ggattccggt aatcggcctc ggcggaatcg cttccggcga ggatgcggcg
     5221 gagtttctga ttgcgggcgc ctccgccgtg caagtgggca ccgccagctt ttgggaccct
     5281 caggcggttt tgcgaatcgc tgctgaactc ggtgcgtttc tcgaatccca gaacattgca
     5341 aatgtcacgg aattggtcgg aacattgaaa ttcagtggag gctgcggttg a
//
LOCUS       DASTMK010000017         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_416914, whole genome shotgun sequence.
ACCESSION   DASTMK010000017 DASTMK010000000
VERSION     DASTMK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416914"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1230
                     /locus_tag="VFQ79_00590"
     CDS             37..1230
                     /locus_tag="VFQ79_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138170.1"
                     /translation="MKWLVRFSLLFVTMAAVAAAGWYAVERLRPVPLAYSTPSAIPEV
                     LLDHGQVKVATNCEFLYASVSTQRDELLAYLEFQYLRGLGLPGSSDVLLTVPRTTFSD
                     RTYRVGLVVENDLLRTIPHLSELKARGFINSFDIRCATRKNIEDKRAQTAVFLGAYNF
                     PVRKKLENLSHAKLRPSVERFILFKSRTDRRVRAGIQPVPPELSPEQASELASDIIEV
                     SRFYSLPLDFFLGIGAMENNYMNVRGDLEHAVWKRRIEPGDIVLKRRRGRVLVSNYAI
                     GMWQITRETLRYAHKLYLKDSRDYSRLSLRLRPGAELEFDSVDSHKLTTYAGLFFRDL
                     LDRFDGDAALAAGAYNGGVANPNLGYTAGARAAANHARKVIEQMAALDGHPVTQTRFF
                     MPGRQ"
     gene            1274..2437
                     /locus_tag="VFQ79_00595"
     CDS             1274..2437
                     /locus_tag="VFQ79_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0138171.1"
                     /translation="MPSDERVAPPAIEPVREPASRLPPRGTLLIVFAVLAALFTLTGI
                     LASAFHSAEKARAAAHFEAGNQFAARGMNDDAIAEYRAALALDHSRTQYRHALATALF
                     NSGSLKEAEARLAELIALDPSSGPINLMLARIAARGGDLSAAESYYHRSIYGLWPQDP
                     QQYRQKTRFELVELLLRNGTKKRAEAELLTLADEVGEDAGTKQRIGELFLAADSPQNA
                     AEVFRELVKQDSRNATLFSALGTAEMRLANYRLARNALRTAVRLDSKDEEAARQLQLV
                     TDVLTLDPTARGLSQRERHRRSLELLRRSSADAARCAAQLGEQIPAGLKETLDHAAKE
                     LESRQRAVDETIDANIALSEALFRLREEHCPGLAEPDRAVAFTIRRLPAEEAP"
     gene            2635..3786
                     /locus_tag="VFQ79_00600"
                     /pseudo
     CDS             2635..3786
                     /locus_tag="VFQ79_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
BASE COUNT          679 a         1142 c         1139 g          826 t
ORIGIN      
        1 taggaggttg ttctacataa actatctggc gccgcgatga agtggctcgt tcgattttcg
       61 ttgctgttcg tgactatggc tgccgtggca gcggcggggt ggtatgcggt tgagcggctc
      121 cgtcccgtgc cgctggccta ttcgacaccg tccgccattc ccgaggtgtt acttgatcac
      181 gggcaggtga aggtcgctac aaattgcgag ttcctctacg catcggtaag cacgcaaaga
      241 gacgaactgc tcgcttatct cgaatttcaa tacctgcgtg gtctggggct tccgggctcc
      301 agcgacgtgc ttctgacagt tccccgcacc acgttctccg atcgcaccta tagagtcggc
      361 ctggtagtgg agaatgatct gctgcgtacg atcccccatc tctcggagct gaaagccagg
      421 ggctttatca actctttcga tatccggtgc gcaacacgga agaacatcga ggacaagcgc
      481 gcccaaaccg cggtgtttct cggcgcgtac aactttccgg tgcgcaagaa gctcgaaaac
      541 ttgagccacg cgaaactgcg cccatccgtt gagcgattca tcctgttcaa atcgcgcacc
      601 gaccggcgcg ttcgcgcggg tatacagccc gtgccgccgg agctctcgcc ggaacaggcc
      661 tccgaactcg catcggacat catagaggtg tcgcgcttct actcgcttcc tctcgatttc
      721 tttctgggaa tcggtgcaat ggagaacaac tatatgaatg tacgcggcga tctggagcac
      781 gcggtctgga agaggcgcat tgaaccggga gacatcgtgc taaaacgacg ccgtggacgg
      841 gttctggtca gcaactatgc catcgggatg tggcagatta cccgtgaaac actgcgctat
      901 gctcataagc tgtacctcaa agacagccgg gactattcgc gtttatcgct gaggttgcgg
      961 cctggggctg agttggaatt cgattcggtg gattcgcaca agctgactac ctatgcaggg
     1021 ctgttcttcc gcgatctgct ggaccgcttt gatggcgatg ccgcgctggc tgccggcgcc
     1081 tacaatggcg gagtggcgaa tcctaacctg ggctacaccg ccggcgcacg ggccgcggcc
     1141 aatcacgcca gaaaggtcat cgagcagatg gccgcgctcg acggacatcc tgtcacccaa
     1201 acccgtttct ttatgccggg aaggcaataa cgttccgatt ttttcgaact tactgtttgt
     1261 atgctgagta gagatgcctt ctgacgagcg agtggcgccg ccggccatcg agcccgtccg
     1321 ggaacccgcg tcgcgactgc ctccgagggg cacgttgctc attgtattcg cggtattggc
     1381 ggcgctcttc accctcaccg gtatattggc cagcgctttt cattccgcgg agaaagcgag
     1441 ggccgccgcc cattttgagg ccggcaacca atttgccgcg cggggcatga atgacgacgc
     1501 tattgcggaa tatcgtgcgg ctctcgctct ggaccacagc cgcacgcaat acaggcacgc
     1561 tcttgccacc gccctgttca actcgggaag tctgaaagaa gcggaggcgc gcctggcgga
     1621 gctgatcgcc ttagaccctt ccagcggacc catcaacctg atgctggcgc gaatcgcggc
     1681 gcgggggggc gatctctccg ccgctgaatc ttactaccat cgctccatct acggtctctg
     1741 gcctcaagat ccgcagcagt atcgtcagaa gacccggttc gaactggtgg aactgctgct
     1801 taggaacggt accaagaagc gcgcggaggc ggagcttctg acccttgcgg atgaagtggg
     1861 cgaggatgcc gggacgaagc aacgtattgg agaactattt ctggccgcgg attcgccaca
     1921 gaatgcagcg gaagttttta gagagctggt taaacaggac tcgcgtaacg ccacgctgtt
     1981 ttccgctctc ggaacggccg aaatgcggct ggccaactac cgactggcgc gcaatgcttt
     2041 gcggaccgcg gtgcggctgg actcgaagga cgaggaggcc gcccggcaac tgcaacttgt
     2101 cactgatgtc ctgacgttag acccgacagc gcgagggctc tcccagcgtg aacgccatcg
     2161 ccggagcctg gagctgttac gccggtcttc ggccgatgcc gcccgctgcg ccgcccaact
     2221 gggcgaacag ataccggccg gtttaaaaga aaccctcgac catgcggcaa aagaactcga
     2281 atccaggcag cgggcggtgg acgaaaccat tgacgccaat atcgctctgt cggaagcgct
     2341 atttcgcctg cgcgaggagc attgtcccgg tcttgcggaa ccggatcgtg ccgtcgcctt
     2401 cacgatccgc cgcttacccg ctgaggaagc tccatgacgg gaccgggggc gctgttcagg
     2461 cgcatgagcc tgcccgcgct cggtgagcca caacgcttcc tgctgctttc gatcctgatc
     2521 ggactgttct ccgggctgct ggtagtctgt ttccacgtgg ccattgcatc gctgagttgg
     2581 gccgctttgg gtacgggacc ggatcgcgga atcctgcgta ctttgctttc tccgatgctg
     2641 ggagcggttg cggccactct tctggtgctg ttcttttttc gcatggccgc gggtagcggc
     2701 gtgaaccaca ccaaggccgc catctacatt tcagacggat atataccatt cagcgcggtg
     2761 acgggcaagt tcctcgcctg ttccatttcc atcggctccg gcaactcgct gggacctgaa
     2821 gaccccgccc tgcaaatagg ctccgggatt gcttctttgc tcggccgtat tttccgcctc
     2881 acccgcgaga gtatgcgtct gatcgcaccg gtcggcgcgg ccgcgggtat cgccgcggcg
     2941 ttcaacactc ctatcaccgg cgtcctgttc gtcatggaac aggtggtcgc ctcctggcgc
     3001 gccggggtgc ttggttccat cctgctatcc gccgtgtcgg cagtggtggt cagccgcttc
     3061 ttcctcggag atcaacctct gttcgaagtc ccagggtttg agctgacgca tccttcggaa
     3121 ttgctggtgt acgccggcat gggcatcgcc ggcgggcttc tggcagtgct ttttatgaaa
     3181 gctgtcgcgc tgctgcggga gaagacacac cacctgccca aatgggcccg ctaggcccgt
     3241 ccgggcgctg ctggattgat ggtgggagta gcgggcatct ggctgcccga agtgatgggc
     3301 gccggctacg gtgccattgc cagcgccttg catgaccagc ttctctggcc gcggctgctg
     3361 gtccttggtt tcgtaaagat gctggtcacg ctgctatgct tcagcgcggg cattcccgga
     3421 ggaatgtttg caccggcgct gttcatcggc gccatgattg gcggcggcat cggcgcgctc
     3481 gcgcacctgt attggccgtt tcccaccagc gccgcaagcg cctatgtgct ggtgggcatg
     3541 ggcacgttct tcgctggtgt ttttcgcgca cctatgacct ccatcttcat ggtgttcgaa
     3601 gtaagcgcca gttacgtcat catcctgccg gtcatgatct ccaacaccat cgcctatctc
     3661 atctcgcgca gcctggcccc gctttcgttt ttcgacatga tcgcccggca tgaaggagtc
     3721 gacctgcctt cgctcgaaga gcatcgcgaa aagcgggacg tgcgcgtcga ggatgcgatg
     3781 cgtatc
//
LOCUS       DASTMK010000018         6102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_1263, whole genome shotgun sequence.
ACCESSION   DASTMK010000018 DASTMK010000000
VERSION     DASTMK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..867
                     /locus_tag="VFQ79_00605"
     CDS             37..867
                     /locus_tag="VFQ79_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEU0138172.1"
                     /translation="MRNLREGSSGPDVVRLQERLKELGFNPGATDGQFDLGTEAAVLA
                     FQRSKGLLADGIVGPRTFRALGLDLEVTIPSVIPAVTVAVVSRMFPVTPIDNIKHNLP
                     FVLDALVNTELVDRKMVLMALSTIRAETESFRPVSEGLSRFNSSPGGRPFDLYDFRQD
                     LGNQGPPDGERYKGRGYIQLTGRFNYGKYGQLIRLGNRLLEEPELANQPEIAASLLAS
                     FLKDKEIPIKQSLLEGDLRRARRLVNGGAHGVDRFVDSFEIGLKLIPEELGVAAPARA
                     "
     gene            919..3432
                     /locus_tag="VFQ79_00610"
     CDS             919..3432
                     /locus_tag="VFQ79_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HEU0138173.1"
                     /translation="MIFRTAVVLLCLVQFVRPAVPTPEEHLGYKPGADYKLADYSDII
                     TYFQKLTASSDRVRLVEFGKSSHGKPAYVAFISSAENLRQLERYRDISRRLALALVED
                     EEAQRLASEGKAVVWIDSGLHATEVAPAQHAPELAYRMLTNEDAETRRIRENVILMQV
                     PVINPDGLDMVVKWYRNNVGTPHELAPLPWLYQKYSGHDNNRDWFMLNLQETRNVTRL
                     LFQEWFPQIVYNQHQQPAFPARIFVPPYAEPLNPNIPATVMEGINAIGAAISERFARE
                     NKPGVLSYFGYDGWWNGGLRSVPAFHNMHGILTETAAHSYATPREYDVKELPERFGNG
                     MPTKHPTIFYQRPWMGGKWTVRDAIEYMLTADWAILHHAAARHSEFLLKSYQMARAQI
                     EAGTRGKPFAYVIPEEQWDRSSALEMMKRLAAGGIVVQRAKAPFQAGGKTFSDGAYVL
                     QAAQPFRPYLVDLMEPQRYPEIRTGSDGPTKRPYDIAGWTLPMQMGVQVQRIEEPFQA
                     QLVPVETVPELAPSLDQRENSSFLAIAGLLNRKTKVRRAADGAILVEGQSPAGSFSKA
                     SWELRKPRLALYEPWTANMDTGWTQWVLDTYQIEHTLLHNEDIRKGGLNAKFDTILFA
                     SQSAASILHGTREGEKIASPQPSLGEAAQGISQQRHEYAGGIGISGLYQLDSFVRNGG
                     TLIALDAAAELPAHYFPLPVRSATAGPRGSDGYYCPGSLLRITVDNTNPIAFGMPEGA
                     IAFSSGGHAWDITLLPEYNRDGRRVRSVARYAKSSLLASGWLSGEKVVLGKEILVEAR
                     HGNGKVVLFGFRPQFRGQPFGTFKFLLNAIYLGSAQKLD"
     gene            complement(3440..4282)
                     /locus_tag="VFQ79_00615"
     CDS             complement(3440..4282)
                     /locus_tag="VFQ79_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0138174.1"
                     /translation="MVRQTRRQVLGSAFAAAFLNAQTPARVTRYVRFARAQQTSHGIL
                     DGNVIREIKGDLFGSHKETGAKYNLSDVKLLFPCQPPQLLAVGLNYRSHIGNQPVPKR
                     PEIFLKPISALQNPGDPIVIPAGAENVHFEGEMVLIIGKRVSKASVDEARDAIFGVTC
                     GNDVSERDWQGGPDKDMQWWRAKGADTFAPMGPSIARGLDYGKLLLQTRLNGKVVQKQ
                     YTSDLLFDGPAIVSWISRAVTLNPGDTIYTGTPGQTSRMKPGDVVEIDIEGIGVLRNP
                     VKSA"
     gene            complement(4323..>6102)
                     /locus_tag="VFQ79_00620"
     CDS             complement(4323..>6102)
                     /locus_tag="VFQ79_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138175.1"
                     /translation="GIALDSEDALFIADGRGNQTLKISPAGIVSYLPVGSRNLAVSAA
                     GELYTVAGQTVQQLLPDGTVHIVAGQNSFARNGDGGPALEARLNMPSALAIDPDRNIY
                     IADRVNNRVRKIDSSGVITTIAGTGERAYKGNGGPATLSALSLPGGLCLDSAGTLFVS
                     DTDNHVVRAIDKIGVIRTVAGSGEPGFAGDQDSATNALLTSPRGLALDPLGRLLIADS
                     GNHRIRRIDQDGRITTIAGTGIAGFSGDGGAATEAQLIAPASIAVDSTGAVFIADTGN
                     HRIRRIAPDGIIETLINREVAGLNLPTAVAVDPEGRLWIADAGNHVIRVWKPDGSIET
                     VAGTGEQGFNGEEGPGNKMQLSAPADIAVAPDGVVLFSDSSNHRIRALTPASETDPVE
                     PEPLGQIRVVNAASLREGPVTSGEIVSVLDGPALAAPQVTFDGTPAQVLFASTGVLQV
                     LVPDLGGRSETEMTLADGEFPVASLTLEVAASAPGIFTISGASGPASATNEDGSQNSA
                     SNPAPRGSILTLYATGTSLPDETPQVRVAGLPAEVLFAGPAPGFGGLLQINLRLPAGY
                     VPSGEVPVTLTSAGVASQPGVVVYLK"
BASE COUNT         1204 a         1814 c         1880 g         1204 t
ORIGIN      
        1 agggagcggt ggctgcaatt ataaagggtc ttaaccatgc gcaatttgag agaaggcagc
       61 agcggcccgg acgtcgtacg cctgcaggaa aggctcaagg aactcggctt caatccgggt
      121 gcgaccgacg ggcagtttga tctgggtacg gaggcggcgg ttctggcttt ccagcggagt
      181 aagggattac tggcggacgg catcgtaggt ccgagaacgt tccgtgctct tgggctggat
      241 ttggaagtga ccattcccag cgtcattccg gccgtaaccg ttgccgttgt gtcgcgcatg
      301 ttcccggtca ctcccatcga caacatcaag cacaatcttc ctttcgtgct cgacgcgctg
      361 gtgaacacgg aactggtaga tcgcaaaatg gtgctgatgg cgctgtcaac cattcgagcg
      421 gaaaccgaaa gtttccggcc cgtgagcgaa ggcttgtcgc gcttcaacag ctcccctgga
      481 ggccgtccct tcgatttgta cgactttcgc caggacctgg gaaaccaggg cccgccggac
      541 ggcgaacgct acaagggacg gggatacata cagctcaccg gccggtttaa ttacggcaag
      601 tacggccagt tgatccgttt gggcaatcgc ctgttggagg aaccggagct ggccaatcag
      661 ccggaaattg ccgccagtct gttggccagt ttcctcaagg acaaagagat tcccatcaaa
      721 cagtccctgc tcgaagggga tttacgaagg gcgcgaaggc tggtgaatgg cggcgctcat
      781 ggcgtggacc gtttcgtcga ctctttcgag atcgggctca aattgatccc ggaagaactc
      841 ggcgtggcgg cgcccgcaag agcgtagctc accccccgag agctgatcag cgggacgccg
      901 atgttatgat ctccgattat gatctttcga acggccgtcg tccttctctg tttagtccag
      961 tttgtccgtc cggccgttcc aactccggaa gaacacctgg gctataagcc cggcgccgat
     1021 tataagctgg ctgattattc ggacatcatt acttatttcc agaaactgac cgcatcgagc
     1081 gaccgggttc gcctggtcga attcgggaag tcgtcccatg gaaagccggc gtatgtcgca
     1141 ttcatttcgt cggccgaaaa tctccgtcaa ctggagcgat accgggatat ctcacgccgc
     1201 ctggcgctgg ctttggttga ggacgaggag gcgcaacggc tggcatccga gggcaaggcg
     1261 gtggtgtgga tcgactcggg cctgcacgcg acggaagtgg cgcccgcgca gcacgcgccg
     1321 gagcttgcct atcgcatgct gacgaacgag gatgcggaaa cgcggcgtat ccgggaaaac
     1381 gtgatcctca tgcaggtgcc ggttatcaat cccgatggcc tggacatggt cgtcaaatgg
     1441 tatcggaaca acgtgggaac ccctcatgag ctggcgccgc tgccatggct ctatcagaaa
     1501 tactcggggc atgacaacaa ccgcgactgg ttcatgttga accttcagga gacgcgaaac
     1561 gtaacccggc tcctgtttca ggaatggttc ccgcagatcg tatataacca gcatcagcaa
     1621 ccggcgtttc ccgctcgcat cttcgtgccg ccttatgccg aaccgttgaa tccgaacata
     1681 ccggctacgg tgatggaagg catcaatgcg atcggcgccg ccatttcgga gcgatttgcg
     1741 cgcgaaaaca aacccggggt actttcctat ttcggctacg acggctggtg gaacggcggc
     1801 ctgcggtcgg tgccggcttt tcacaatatg cacggcattc tgaccgaaac ggcagcacat
     1861 tcctatgcca ctccccgcga atacgacgtg aaagaactgc ccgaacgatt cggcaacgga
     1921 atgccgacga aacaccctac gatcttctat cagaggccgt ggatgggcgg caaatggacc
     1981 gttcgggatg cgatcgaata catgctgacc gcggattggg ccatcctcca tcacgccgct
     2041 gcgcgccact cggagtttct gttgaagagt taccagatgg cgcgtgcgca gatcgaggcc
     2101 ggcacacgtg gcaaaccgtt tgcctacgtt attccggagg agcaatggga ccgctcgagc
     2161 gccctggaga tgatgaaacg tctcgccgcc ggcggaatcg tggtccagcg tgccaaggcg
     2221 ccgtttcagg ccggtggaaa gacattctcc gacggcgctt atgtgttgca ggcagcccag
     2281 ccgttccgtc cgtacctggt ggacctgatg gagccgcagc ggtatccgga aatccgcacc
     2341 gggagcgacg gtccgaccaa gcggccctac gacatcgcag gctggactct ccctatgcag
     2401 atgggcgtgc aggtgcaacg gatcgaggag ccgtttcagg cgcaactggt tccggtggag
     2461 accgtgccgg aactcgctcc gagtctcgac cagcgggaga actcctcctt ccttgccatt
     2521 gccgggctgc tgaaccggaa gacgaaagtt cgccgcgccg ccgacggtgc gattctggtg
     2581 gaaggccaat cgcccgccgg cagtttctcg aaggcttcct gggagcttcg caaaccgcga
     2641 ttggcgttat acgaaccctg gactgccaat atggacaccg gctggacgca atgggtgctc
     2701 gatacgtacc agatcgagca caccctgctc cacaacgaag acatccggaa gggcggcctg
     2761 aacgcaaaat tcgatacgat tctcttcgct tcccagagcg ccgcttccat tttgcatggt
     2821 acgcgtgagg gggagaagat cgcttctccc cagccgagct taggggaagc ggcgcaaggc
     2881 atctcgcagc agagacacga gtatgcgggt ggaatcggaa tatccggact gtaccaattg
     2941 gattccttcg tgagaaacgg gggcacactc attgcgctgg acgccgccgc ggaactgccg
     3001 gcccactact tcccgctgcc ggtgcgcagt gcgactgccg gtccccgcgg gtcagacggc
     3061 tactactgtc ctggctcgct attgcgaatc accgtggata acaccaaccc catcgcattc
     3121 gggatgcccg agggcgccat cgcgttttca agcggcggcc atgcgtggga tattacgctg
     3181 ctgcccgaat acaatcgtga cggcaggcgg gtccggtccg tcgccaggta cgcgaagtcc
     3241 agcctgcttg ccagtggctg gctgtctggt gaaaaggtgg tgttggggaa ggaaatcctg
     3301 gtggaggcac ggcacggcaa tggtaaagtg gtgctcttcg ggttccggcc ccagtttcgc
     3361 gggcagcctt tcggaacgtt caagttcctg ttgaatgcga tctatcttgg ctcggcccaa
     3421 aaactcgatt gatcggcgat caggccgatt tcacggggtt ccgaagtacg ccgatgcctt
     3481 cgatatcaat ctcgacgacg tcacccggct tcatcctgga ggtttggcct ggagtgcccg
     3541 tgtaaatggt atcgcccggg ttcaacgtca cggcgcggct gatccagctt actatcgccg
     3601 gtccgtcgaa gagcaggtct gacgtgtact gcttttgcac gactttaccg ttcagccggg
     3661 tctgaagcag gagcttgccg taatcgagcc cacgcgcaat cgacggaccc atcggcgcga
     3721 acgtatcggc tcccttggcc cgccaccatt gcatgtcttt gtcgggtcca ccctgccagt
     3781 cgcgctcgct cacgtcgttt ccgcaggtaa cgccgaaaat ggcatcgcgc gcttcgtcga
     3841 cggacgcttt gctcacgcgc ttgccgatga tcaggaccat ctcgccttcg aagtggacat
     3901 tctccgcgcc cgcggggatg acgatcggat cgccggggtt ctggagcgcg gagatgggct
     3961 tcagaaaaat ctcgggccgc ttgggaacgg gctggttgcc aatgtggctc ctgtagttca
     4021 aaccgacggc gagaagctgt ggcggctggc agggaaagag cagcttgaca tcggaaagat
     4081 tatacttggc gcccgtctct ttatggctgc cgaacaggtc gcctttgatt tcacgaatta
     4141 cgttgccatc gagaatgcca tgggaggttt gctgcgctcg cgcgaaccgc acatatcgtg
     4201 tcacacgggc tggggtttga gcgttcagga acgccgccgc gaatgccgag ccgagcacct
     4261 gccttctggt ttgtcgcacc atgtgcacca gtttacggcc aaaggcgcaa cctggggcag
     4321 cgttatttca aatacacgac gacgccgggc tgcgaggcaa ccccggccga cgtcagcgtt
     4381 accggcactt ctcccgaagg cacgtatccg gcgggcagcc tgagattgat ttgaagcaag
     4441 ccgccgaagc ccggcgccgg acctgcaaac aggacctcgg caggcaggcc tgcgacgcgt
     4501 acttgcggag tttcgtccgg caggctggtt ccggtggcgt acagagtaag aatggaacct
     4561 cgtggcgcgg gattcgatgc cgaattttgc gagccgtcct cgtttgtagc tgaagcggga
     4621 ccgctggctc ccgatatcgt gaagatgcct ggtgcgctgg cggccacttc gagcgtcagt
     4681 gaagcgacag gaaattcacc gtccgcaagg gtcatctccg tttcggatcg gccgcccaaa
     4741 tccggaacca atacctgcaa cacgcccgtg gaggcgaaaa gcacttgcgc cggggtaccg
     4801 tcgaaagtca cctgcggcgc cgcgagggcc ggcccgtcca gaacagaaac gatttccccg
     4861 gaagtgactg gtccctcccg gagcgaggcc gcatttacca cccgaatctg tcctagcggc
     4921 tcaggctcaa cgggatcggt ctccgaagcc ggggtaagcg cgcgaatgcg atgattgctt
     4981 gagtcggaaa acagaacaac gccgtcaggg gcaacagcga tgtcggccgg tgcgctgagt
     5041 tgcatcttgt tgcccggacc ctcttcgcca ttgaagcctt gctcgccggt tccggcaacg
     5101 gtctcgatag acccgtccgg cttccatacc cggatcacgt ggttacccgc atcggcaatc
     5161 cagagccgcc cctcggggtc gacggcaacg gcagttggca aattcagccc cgctacttcc
     5221 ctgttaatga gcgtttcgat aatcccatcg ggcgcgatgc ggcgaatccg gtgattcccc
     5281 gtgtccgcga taaacacagc gccggttgaa tccaccgcaa tggaggcagg agctatcaat
     5341 tgcgcctcgg tggcagcgcc tccgtcgccg gagaaccccg ctatgccggt acccgcgatc
     5401 gtggtgattc tcccgtcctg gtcgatgcgg cggatgcggt gattgcccga atccgcgatg
     5461 aggagtcttc cgagagggtc cagtgcgagc ccgcggggag aggtgaggag cgcgtttgtt
     5521 gcggagtcct gatcgcctgc gaaacctggt tctccgctgc cggccacggt ccggataacg
     5581 ccgattttat cgatggcgcg gaccacgtga ttatccgtat ccgacacgaa gagcgttccg
     5641 gcggaatcaa gacaaagtcc accgggcaaa ctcagagccg acagcgtggc cgggccgccg
     5701 tttcccttgt atgcgcgctc gcccgttccg gcaatcgtgg tgatcacacc cgaggaatcg
     5761 attttccgca cgcggttgtt tacccggtcg gcgatataga tattccggtc cggatcgatg
     5821 gcgagcgcgg agggcatgtt gaggcgcgcc tccagcgccg gaccgccgtc cccgttgcgg
     5881 gcgaagctgt tctgtcccgc cacaatgtga acggtcccat ccggcagaag ctgctggacc
     5941 gtctggccgg ctacggtgta aagctcgccc gctgcgctta ccgccaggtt gcgcgagccg
     6001 acgggaaggt aactcacgat gcccgccggg ctgatcttca acgtctggtt cccccggcca
     6061 tcggcgatga aaagagcatc ctcggaatcc agcgcgattc cc
//
LOCUS       DASTMK010000019        23106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_257126, whole genome shotgun sequence.
ACCESSION   DASTMK010000019 DASTMK010000000
VERSION     DASTMK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 23106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23106
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257126"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFQ79_00625"
     CDS             <1..299
                     /locus_tag="VFQ79_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138176.1"
                     /translation="TFFSAGWVLLMLIAFYWLVEVKGYKRWTFFAVVVGANSIFIYSF
                     AQVLRGWLNRGLGRFTGNFGFLGDLGAIPQNLLVMFVMWYLCYWLYKRKIFIKI"
     gene            424..795
                     /locus_tag="VFQ79_00630"
     CDS             424..795
                     /locus_tag="VFQ79_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0138177.1"
                     /translation="MTLDVMIVDDSAAIRKILQRVLRQAEVPIGEVFEAGDGREALGM
                     LETRSVGLILSDINMPNMDGLEFLGQLKANNHLKSVPVVMVTTEGSQAKVMEAVELGA
                     AGYVRKPFTAEQIKEKLTGII"
     gene            812..1342
                     /locus_tag="VFQ79_00635"
     CDS             812..1342
                     /locus_tag="VFQ79_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HEU0138178.1"
                     /translation="MRGLDHSKVVHAICDSTREVFSTMLGMEIEAGEFYFQQSVPGEN
                     EGVVSLIGLAGDWAGSGSISCSPEFARHISSALLMTEFASVDEEVLDAVAEVTNMIIG
                     NVKNSLEEDLGPMGLSIPTVIFGRNFTTRSVGNSDWIVVPFQSGNEQMKVQICVAPSK
                     TKHPPRPGFANDLALA"
     gene            complement(1365..4622)
                     /locus_tag="VFQ79_00640"
     CDS             complement(1365..4622)
                     /locus_tag="VFQ79_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138179.1"
                     /translation="MIRLAAIALACFFGTAAFGQTVQVSPSKLTFNVAAGSIPQPQTF
                     TVTTNPAGFSFSIGNSSELLSVSTLSGVTPATIAVTPNTALLTPGSRHTLGLFIRVQG
                     AIEQLLIVELNVGFGSQLTASQPGLAFSYQADSPVIPQTQSLTIASLTGFPLSFTAAS
                     SAPWLTVNPANGNTSTANLLVAGVSPQDLNEGAYNATITVTPQTIPDAVPLQIPVTFT
                     IGGAPQLNVSRSSLLFNYQSGIGATAPVPQTISFTSSTDTQVPFSIVRGPATWLVLTT
                     VNGFTPAELSLSVNPAGLPPGNYSETLTISAPGASNPTQNVTVTLRVSNSPLLNTSPA
                     SLSFTYQSGGELPPAQTVTVTSTTPSTPLGFSSAFTPASGGQWLTLTTATATTPGSIL
                     AGLNGPVVSGLLPGTYSGTVAVTGAGGDITNIPVSLNVTNDPVLTSTPTGLTFVYQRG
                     KSLPAGQFVDVRSTGIPLTFTASATSADQWLTVVPLTAATTPAQIFVSVNPAQLTAKE
                     YDGSITLSPQSTGTAAVTIPVKLIVSDTPLLRISPTELIFTLSAEGGSLLETQLISLE
                     STDPAAPLQYNVSGAPTTGTNWLSIGLPVSGSTPNNVRVTVNATGLSAGTYTGSVSIA
                     ATGANTQTVPVRLIISSGTVTVSPAQLSFTQASGGAAPPSQKISVESSGGNLGFTATP
                     RASAGNWLKVEPTSGTTPASLTVTADGAGLSPGTYSGDITIQAAGSSTPRIVPVTLTV
                     TQAQSITVEKKSLAFNYSVGGSAPAAQTVAISGSGQNMSFTAAASMTSGTGWLAATPA
                     TGTLPGTVTVTVNVSGLAVGTYNGRVRVSSPAASNSPQDIEVSLVVSAGLPSPVAVVN
                     AASFAPSAIAPGEIVTVFGTNIGPPTLTPLKITNGLVDTELAETRVLFDGIPAPLIYV
                     RNDQLSAVVPYALTGRFSTRMQVEYREVRSEAIELRVADTAPGIFTFTVSGSGQGAIL
                     NQNYSDNGMAAPAARGSEVMIYLTGAGQTTPLGAEAAIAPAAGAQKRVNAEVRVIIGG
                     RQAEVTYAGTAPGNTSGLTQVNAKIPTDVQPGNAVPVQVIIGGISSQSGVTMAVQ"
     gene            complement(4796..6301)
                     /gene="glpK"
                     /locus_tag="VFQ79_00645"
     CDS             complement(4796..6301)
                     /gene="glpK"
                     /locus_tag="VFQ79_00645"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU0138180.1"
                     /translation="MPYILALDEGTTSARAALYDREARRIAIESAPIPCRYPQSGWVE
                     QDAEDIWVSQIESARRVLETASVAPSALVALGITNQRETTIVWDRITGQPVAPAIVWQ
                     CRRTAAECDEIRRSGSAAAISAKTGLVIDAYFSATKLQWILRAVPDAYAKARDGEILF
                     GTVDTWLIWRLTNGAVHVTDTSNASRTMLMNLESGDWDRELLDYFDVPRAMLPRIVPS
                     SGVVGVAATEHLGAEVPIGGIAGDQQAALFGQACFRPGLCKNTYGTGCFALMHTGAHR
                     PVSGNQLVATRAAGSGDSPAYAIEGSIFIAGAAIQWLRDKLGLIRTAAESETVAASVP
                     DTDGVYLVPAFVGLGAPHWDASARGVVCGITQATSAQHIVRAALESIAYQTRELVEAM
                     EADSRESLSELRVDGGAAANNFLMQFQADILGRRLVRPVDTETTALGASFLAGLAAGF
                     WKDSGELEQFWQPQRVFEPRMQVERREELYSGWKSAVRRCRLDAASQAVAS"
     gene            6324..6770
                     /locus_tag="VFQ79_00650"
     CDS             6324..6770
                     /locus_tag="VFQ79_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138181.1"
                     /translation="MFFRREQPQQLTFDERIGRAQKAGFSAEKHPDGRVRLSKDGCAA
                     IVEDVPGPRPLVERAGILIGDEIGKLVHGGYQEFLLTPSGRKLPALARHLRVLHAFQE
                     DLREALGLVSLYNESLGTVNDSHMYDRLEDRDRGGRPRPWEQRIVS"
     gene            6826..8091
                     /locus_tag="VFQ79_00655"
     CDS             6826..8091
                     /locus_tag="VFQ79_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEU0138182.1"
                     /translation="MKQKAFLIPLLLAQACLGASGVVGVTVHGVVHPITTEIVGHAIE
                     QAKVQDAALLLIRINTPGGLMEATREITEKIVTSPIPVVTYVAPSGARAASAGFFILE
                     SGDVAAMAPGTNTGAASPIVMGREMDPVMRKKVENDAAAQLRTLAGRRGRNSELAEKA
                     VFEAKSFTEEEALKSQLIELIATDDRDLLQKLDGREIVRFSGARSTLQVASAEITEYQ
                     PSLRERVMLALANPNTAFILLILGALGIYVEFSSPGLIFPGVLGGILAILGLSALSVL
                     PINWAGAALIVLALALFVLEANVTSHGILGIGGAVAMVLGALLLIEGPPEMRINLTTA
                     IAVTLPFALITIFLVSLVMRARKAKVVTGDIGMVGEAGHALTPLSPGGKVFVRGEYWD
                     AIATVPVEAGAQVIVTAVKGLRLTVEPFR"
     gene            8111..8881
                     /locus_tag="VFQ79_00660"
     CDS             8111..8881
                     /locus_tag="VFQ79_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEU0138183.1"
                     /translation="MLDATTIVLIIIAIYLLSSIKILAEYERGVVFRLGRVLAQPKGP
                     GIILVFAPIDRMVRTSLRIETLEVPSQDVVTRDNVTVKVNAVIYFRVLDPNKAVLEVT
                     NYLYATSQLAQTTLRSVLGEVELDELLSQREKLNVRLQSVLDQHTGPWGVKVSMVEVK
                     QVDLPEIMIRAIARQAEAERERRAKIIHAEGEYLAAEKLAMAAELIQHQPAAITLRYL
                     QTLVEIGSEKNTTVVFPLPIDIIPILRKAITETVQPKQ"
     gene            8885..9436
                     /locus_tag="VFQ79_00665"
     CDS             8885..9436
                     /locus_tag="VFQ79_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU0138184.1"
                     /translation="MPRNEEGEFELVLGNKQLLSLFFIVVVLLGVFFTMGYIVGRSSS
                     PGPDLASAPEAVSSAPPAEAPRESPAEAAYEPPSQRTVEVREAAAPPTPAPTPPPRER
                     PLFVEPAAGETYLQVMAVARPDAELVAEALRKKGLAAAVAAGPSPAFYRVLVGPLSDT
                     ASIASARAEIEGAGFKNPIVRKY"
     gene            9438..10343
                     /gene="lipA"
                     /locus_tag="VFQ79_00670"
     CDS             9438..10343
                     /gene="lipA"
                     /locus_tag="VFQ79_00670"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU0138185.1"
                     /translation="MPGDLVHIHTGHARLPHWLRKPETHFESVQHLKRNLRRLNLNTV
                     CESARCPNMHECFHRGAATFMILGNLCTRGCGFCSVPKGSAAKREFFLDPGEPANVAR
                     MAEAMRLRYVVITSVNRDDLPDGGSRHFAETVREVRRALPEAQVEVLTPDFCGDLEAA
                     GRVLDASPHVFNHNMETVAALYRKVRPQARYDQSLAVLRYAAKHNPAILRKSGLMVGL
                     GETAGQVHELLRDLNEAGVDVVTIGQYLQPTRRNLPVAKFVTPEEFDAYRDYGLSLGF
                     KMVFSGPFVRSSYMADLVNTRAREC"
     gene            10340..11818
                     /gene="lnt"
                     /locus_tag="VFQ79_00675"
     CDS             10340..11818
                     /gene="lnt"
                     /locus_tag="VFQ79_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU0138186.1"
                     /translation="MNFLFAAVTAILLILTVPRFEIGWLAPVALVPLIFALAREPRWR
                     WRFLLGWLAGVLYWFGVCYWIQGVLYQYGGLGQFAAWASFLLFCAAKALHLAVFSALA
                     GYVLRAWYAIPAVAALWTGIERTHGPLGFTWYLLGNSAVDMEAPMRLAPYVGIYGLSF
                     ILVMMNVAVVLVMLRRPRREILWLVSLPLLYLLPNMPLPEKPERVAAVVQPNIAEETR
                     WTPESADAMQRSFELLSLQAALKGDEPPVDLVIWPEVPAPLYYFEDPVLRKRVAGVAR
                     LADAHVLMGTVAHAPDASPLNSAVMVKPDGEAEGRYDKIFLVPFGEFVPPLFGFINQI
                     TTEAGEFKPGEKVVTFPVNGQGVGPFICYESVFPHLVRKITGNGAHLLVNLSNDGYFA
                     RSAAREQHLNIVRMRAAENRRWIIRATNDGITVSIDPAGRIRQRLPAHERLSGRLSYS
                     YLSDVTFYTQYGDWFAWSCLAAGLLLVLVSQIPVYRPEEPRR"
     gene            complement(11871..13004)
                     /gene="bshA"
                     /locus_tag="VFQ79_00680"
     CDS             complement(11871..13004)
                     /gene="bshA"
                     /locus_tag="VFQ79_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEU0138187.1"
                     /translation="MKIGITCYPTYGGSGIVATELGMELAYRGHEIHFISYANPIRLD
                     SGTPRIHYHEVEVTTYPLFEYPPYCLALASRMAEVAESYKLDLLHVHYAIPHSISALL
                     ARDMMVSQMPSHRRLPFITTLHGTDITLVGADRSYFPITKFSIEKSDGITAISDYLRT
                     RTIEVFGVEKDIRVIKNFVNCELYRPDPERKLASELAPPGAAILMHVSNFRPVKRPLD
                     CVRILREVIQETPAHLLMVGDGPERGPAERLARELGVENNVSFLGKQAHVERLFPNAH
                     VLLLPSELESFGLAALEGMACGVPPVATRAGGLPELVTHGTDGFLEDVGDVSAQAARV
                     VSLLKDRDLLGKMGAAARLTALTRFCTSLIIPAYEEYYREICG"
     gene            complement(13005..14027)
                     /locus_tag="VFQ79_00685"
     CDS             complement(13005..14027)
                     /locus_tag="VFQ79_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138188.1"
                     /translation="MADGLRAGLAAALLLASGCGSYGEFALSQPTSEPQQGRFLFEQE
                     ALPVLERGPAGRWDSADVLNPSIVRHVNQYFNFYSGFDGKTWHTGLAISPDGSGWLRR
                     GKLLSPGPNAWESDYISANGSALWHEGEWFYWYQAGPRNANRIGLARSRDLASWSRVA
                     QPVLPAGPRGSWDEISIGDPYVLRRDGFFFMYYLGMDRARRQRLGIARSRDGVRWTKL
                     RSNPVLEPGEPGEFDDNGLGEPAVWESHGFYWMIYTGRDRSEIRRLGMAQSRDGVRWN
                     KRRDLVFSGEAAWNQAVLCDPDVEVLGDRIRVWFGGGDRPSPDENLNGAIGIGMLRFQ
                     DASLTK"
     gene            complement(14020..15123)
                     /locus_tag="VFQ79_00690"
     CDS             complement(14020..15123)
                     /locus_tag="VFQ79_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU0138189.1"
                     /translation="MTRIFIALSCLVFQIQWSERFSGLSFLLALYLAGAIATLFWRTA
                     DRAAQPLLILFADFGIFLIWTYAAAGRFLFVTAAFFVYIVLRISLLHSWYELSFLLGV
                     LAVFLGAARPIGASGIWLMMLCAGTVGLVLTLHREILQNRLSSTARQSVILRAEAEKS
                     REEERQRIAADFHDGPLQSFISVQMRLEIVRKMLMRDFQRGMDELRQLQELCHSQVVE
                     LRSFIRSMRPAEIEGAGMITSIRHLVERFQKDSGMTASFHDTASVSGEEPELSTEVLS
                     IVREALNNVQKHSKASRVAVSVSHGDSALDVVIEDDGTGFTFSGNYSLEELELLRLGP
                     DSIKRRIRSLGGDLTLDSRPGSGASMRIRVPHG"
     gene            complement(15212..15745)
                     /locus_tag="VFQ79_00695"
     CDS             complement(15212..15745)
                     /locus_tag="VFQ79_00695"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0138190.1"
                     /translation="MIRSIPVLFLLPYIALSQQTADAEKLAPYYPTPETVVEKMLEFG
                     GLKQGERMYDLGSGDGRIVIMAARKFRANATGVEMDQDLWKQSMDRIRGLGLKNARVI
                     NGDITRQNYSDADLVTVYLLPASNVKIRPMLEKQLKKGARVVSHDFEFTGWTPVKTEH
                     IEDDGEGRSHTLYLYVR"
     gene            complement(15830..17734)
                     /locus_tag="VFQ79_00700"
     CDS             complement(15830..17734)
                     /locus_tag="VFQ79_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138191.1"
                     /translation="MSRNAILIALFVTNILPAQGLRPDWRSIGNTVLDLNLPAPASGG
                     VERVWFAENGARLYVRTAAGRQFETEDLKRWTEPSQPLPPAPEPPTVIRLPEPGAQVR
                     QIRYARARLYAFGEHAWRSDDGGATWRNLTAYHGVSLLGSPIRDLAISPFNASEIVVA
                     GDYGLWRSSDGGESWGGINTGLPNLPVQRILRLPKGVDGMTIELAESGTGAAPAQFEW
                     APGEKIAWRPTIASDTLARERVQKSEYSVLLGARITAVASAREFVYAGAGDGRLWASS
                     DQGRTWFPRQEQSDGPVEQIWIDPSDARIALAVFGPPTSAGGTAQSTARIVKTFNAGA
                     FWDDITANLSGAAVYSITADRTSGAIYAGTSDGVFMTRTDLGALGPATQWTEVSGKLP
                     EAAVRDVRLDAAGNQIYAAIEGYGLFAAPAPHRASEPRLVNAADLNIRAAAPGGLISM
                     IGAQIQSARAGALPVPVLDASAGATQMQIPFEVTGTSITLAMQGTGGRYTLGLPLQKI
                     SPAIFVDPDGTPFLLDGENGLALSATTPARSGTALQILAAGLGKVEPEWPTGLPAPLE
                     NSPAVAVPVRAFLDGSPVEVTRAILAPGYIGMYLVEIRVPAIVNSGPAELYLEAEGQQ
                     SNRVRVYLEP"
     gene            17920..18735
                     /locus_tag="VFQ79_00705"
     CDS             17920..18735
                     /locus_tag="VFQ79_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEU0138192.1"
                     /translation="MARLSTWFEVGLAAALLLPAAFSQIVPGRADQAARVVSLDGQVS
                     VMRDAIPWALKEGDPVRVRELIVTGPDGSAVFELADGSTFEIYPNSHVTFRNTPGNLR
                     DLLDLWLGRIKVHIQKFGGQPNLNRVHTPTAVISVRGTVFEVMVDEEDKTTFVMVEEG
                     QVAVQHALLPSGEPKILNAGEYIQVHKDAPLARRGVDRDTVFKHAFRALIDAMYTAVY
                     RTPRSGGGVSVPGSGGGPQIPGDTGATPAPESVPGPGDVGATPPPPAPPASAP"
     gene            18794..19633
                     /locus_tag="VFQ79_00710"
     CDS             18794..19633
                     /locus_tag="VFQ79_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0138193.1"
                     /translation="MCAIRLLAVMAISSALAAGDGSFTVAGLRLMPKRWDKQANFEKL
                     SYYARKAAERGAEVVVTPEGFLEGYVGNEKANKDLTRDRYFAIGEPVDGEMIGRCRNL
                     AKELGIYLVLGFAERSGDQMRNSAAVISPGGEIVLKYSKSHTADDEPFNTKGREFPVT
                     DTPFGRWGTLICMDRQLPETSRILAVKGAQIILVPAWGMNNETNDIMMRTRAYENGVW
                     VVFVHPERVLVIDPSGKITAEDSREGDEAVLAEIRVDSRIGRGPIRHRRPEIYGEILG
                     QAK"
     gene            complement(19675..19914)
                     /locus_tag="VFQ79_00715"
     CDS             complement(19675..19914)
                     /locus_tag="VFQ79_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0138194.1"
                     /translation="MGNQSKKPSRRVVIGASGVRTIREKTKLSQMEFASLIGVSVKTL
                     QNWEQDRRRPTGPAAALLKIIAHEPQLAVKAIHRA"
     gene            complement(19936..20277)
                     /locus_tag="VFQ79_00720"
     CDS             complement(19936..20277)
                     /locus_tag="VFQ79_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138195.1"
                     /translation="MKRPLVLAARALTLFWAVFWMFFFVVESVVWRTPVSSALPWVGV
                     GLLFVFSAVVPWRWEMIGGLLIIAVGLSAGVAHAIWWPSRLLTTLVFGAPPLAAGILF
                     LTHRRPVTSNA"
     gene            20390..20671
                     /locus_tag="VFQ79_00725"
     CDS             20390..20671
                     /locus_tag="VFQ79_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0138196.1"
                     /translation="MAEKLLTVNEAIRALGVSRSTLWRRLQSGAIPSVRRGGRRLVCL
                     TMTRKATRGEIAGDIPPFTGKHPIFRLIGAGRSGGRTPGARDKHAILDA"
     gene            20668..21084
                     /locus_tag="VFQ79_00730"
     CDS             20668..21084
                     /locus_tag="VFQ79_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138197.1"
                     /translation="MSIKRGVLWDSSAILALLDADDPDHELAVAIAGQIASQRRPSFI
                     TNYIEVEAHALLLRKLGTALAREWLLTGGLPVIRALPEEEDRGREIVARHSDKDWSLC
                     AAISFAILDVRGIRRAFTFDHHFLQYGRIEVLGLSR"
     gene            complement(21252..21767)
                     /locus_tag="VFQ79_00735"
     CDS             complement(21252..21767)
                     /locus_tag="VFQ79_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138198.1"
                     /translation="MSANTKQDRIFRNLLTYIDVQLGRILARKPVPIDLMALVTRNLI
                     EVALWCEFITKNNENMQRFDKDVLVDVVEMFGLVDSSDPEYSSLERRVKQWEVSGKSM
                     RLAKTGTHDKFWFKLCSKMVHPTAWSINVLLSSSCTDFYRLQLGSYALKCAIRAFCSL
                     TALPLPTYITS"
     gene            complement(22007..>23106)
                     /locus_tag="VFQ79_00740"
     CDS             complement(22007..>23106)
                     /locus_tag="VFQ79_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU0138199.1"
                     /translation="VPLTSLVGPLGLLAVLAGWKLPAQAAGLLLDWSRVVVDWHAGWE
                     PNWRIPDPPVIVALAFAACLAAVAFVPHSRKIWKWGAMMGAGILLVVIIWHPFKPDVV
                     PGSFELTAIDVGQGDSLFLALPDGTTMMVDGGGIPTFGGRSAPRLDIGEDVVSPYLWK
                     RSIRRLDAIAFTHLHADHCGGIQAIMENFRPRELWTGATPDIDLWRSIRAKASQLGIQ
                     VKPLRAGTRFSFGGAGVEVLSPPEDYIPGEQPGNNDSLVLRFTYARHSFLLTGDLERR
                     MEYALPVEKTDVLKVAHHGSRTSTGSEFLQALRPAFGIISVGFENSYRNPHPEVIERL
                     EADKTRVLRTDYSGAITIRSDGTRFSLETGL"
BASE COUNT         4616 a         6865 c         7070 g         4555 t
ORIGIN      
        1 tcacgttttt tagcgcggga tgggtgctgc tgatgctgat cgccttctac tggctggtgg
       61 aagtaaaggg ctacaagcgg tggactttct tcgccgtggt agtgggtgcg aactccattt
      121 ttatctactc gttcgcgcag gttctgcggg gttggttgaa ccgcgggctc ggaagattca
      181 ccgggaattt cggttttctg ggcgacctgg gagcgattcc gcaaaacctg ctagtaatgt
      241 tcgtcatgtg gtatctctgc tactggctgt ataagaggaa gatcttcatc aagatctgaa
      301 agactgcccg tttttcgagc cgccggcccg gtgcttttcg aaactctgcc cttttatcgt
      361 ctttattcta aagacctcgc ctattttacc gatgaggaca acagcgcata agcgaggaga
      421 gcaatgacat tggacgtaat gattgtggac gattccgcgg cgatcagaaa gatccttcaa
      481 agggtgctgc gccaggcgga agttcctatt ggagaggtct tcgaagctgg agacggcagg
      541 gaagctctgg gtatgctgga gacgcggagc gtcgggctga ttctatcgga catcaacatg
      601 cctaacatgg acgggctcga atttctgggg cagctgaaag cgaataacca tcttaagagc
      661 gttccagtgg tgatggtgac caccgaggga agccaggcga aagttatgga agcggtggaa
      721 ttgggagccg caggttacgt tcgaaagccg tttaccgccg aacagatcaa agagaagctc
      781 actggaatca tctgagggga cgtgaaaaac aatgaggggg ctcgaccata gcaaagtggt
      841 ccacgcaatc tgcgattcga cgcgcgaggt tttttcgacg atgttgggca tggagattga
      901 agctggggaa ttttacttcc aacaaagcgt gccgggcgaa aacgagggtg tggtttcgct
      961 gatcgggctt gccggcgatt gggcgggcag tggaagcatc tcgtgctcgc ccgagttcgc
     1021 ccggcatatt tcctccgcgc tgctgatgac ggaatttgcc tccgtcgatg aggaagtgct
     1081 ggacgccgtc gccgaggtca cgaacatgat tatcggaaac gtcaaaaaca gcctggagga
     1141 agacctgggc ccgatgggcc tcagtattcc caccgtgatc ttcggccgca atttcacgac
     1201 gcgtagtgtc ggcaacagcg attggatcgt tgtgcctttc cagtccggca acgagcaaat
     1261 gaaagtgcag atctgcgtgg cgccgagcaa gacgaagcat ccgccgcggc cgggattcgc
     1321 gaatgatctg gcgctggctt aaccagcaga aatcatggcg gctgtcactg caccgccatc
     1381 gtcactccgc tttgtgaaga gatcccaccg atgatcactt gcaccggaac ggcgtttcct
     1441 ggctgcacat cggtaggaat tttagcattc acttgcgtca agcccgaggt gttgcccggc
     1501 gccgtcccgg cataagtaac ctccgcctgt cggccgccga tgataacccg cacctccgca
     1561 ttcacccgtt tctgcgctcc cgcggctggt gcgatggccg cctctgcgcc taggggcgtt
     1621 gtttgcccgg cgccagtgag atagatcatc acctcggagc cgcgcgccgc tggtgcagcc
     1681 ataccgttat ccgagtagtt ctggttgaga atggcgccct gtcccgaccc agataccgtg
     1741 aacgtgaaga tcccgggggc agtgtcggcc acacgcagct cgatggcctc cgatcgtact
     1801 tcacgatact ccacttgcat cctggtgctg aaccggcctg taagcgcgta cggcactact
     1861 gcgctcaact ggtcgttgcg gacataaatc agtggagccg gaattccatc aaacaagaca
     1921 cgcgtctcag caagctccgt gtccaccagg ccgttggtta ttttcaaggg ggtgagagtg
     1981 ggcggcccga tgttcgttcc gaagaccgtc acaatctctc cgggggcgat ggccgatggc
     2041 gcaaagctcg cggcattcac aacggctacc ggcgacggca ggccggccga gaccactaaa
     2101 gacacctcga tatcctgggg gctgttggac gccgccgggg aagacacgcg gacacggccg
     2161 ttgtaagtgc ctaccgccaa accggagaca ttcacggtca ccgtcacagt tcccggcagc
     2221 gttcccgtag cgggtgtggc cgcaagccat ccggttccgg acgtcatcga ggcggccgcg
     2281 gtgaaactca tgttctgtcc cgacccgctg atggccacgg tctgggccgc gggtgcggag
     2341 ccacccaccg aatagttgaa cgccagggac ttcttttcga cggtgatcga ttgcgcctgc
     2401 gtcaccgtca aggttaccgg cacaatccgg ggcgtagaag aaccggcggc ctggatggtt
     2461 atgtcacccg aatatgttcc gggcgagaga ccggctccgt ccgctgtaac ggtcaggctt
     2521 gccggcgtgg tcccgctggt tggctccact ttcagccagt tgccggcgga tgcgcgcggc
     2581 gttgctgtaa accccagatt cccgcctgag ctttccaccg aaattttctg ggacggtggc
     2641 gccgcgccgc cggaggcttg cgtaaagcta agctgtgccg gcgatacggt aacggtgccg
     2701 ctggaaataa tgaggcgaac cggaacggtt tgtgtattgg cgccggtcgc cgcaatggac
     2761 acggacccgg tatacgtgcc ggcggaaagg cccgtcgcat tcaccgtcac ccggacgttg
     2821 ttgggcgttg agcctgacac cggcaggccg atactgagcc agttggttcc cgtcgtagga
     2881 gcgccggaaa cgttgtactg cagcggcgcc gccggatctg tgctctccag gctgataagc
     2941 tgtgtttcaa gcaacgatcc gccttccgcg ctcagagtga agatcagctc ggtcggggag
     3001 atccggagca gcggagtgtc gctcacgatc agcttcaccg gaatggtgac tgcggcggtt
     3061 ccggtgctct gcggtgaaag tgtaatcgat ccgtcatact cctttgccgt gagctgggcc
     3121 gggttgacac tgacgaagat ttgcgccgga gtagtggccg ccgttagcgg cacaaccgtc
     3181 agccactggt ccgcgctggt cgccgacgcg gtgaaggtaa gcggaattcc ggtactgcgc
     3241 acatcgacga actggccggc gggaagcgac ttgccccgct gatacacgaa cgtgaggccg
     3301 gtaggcgtgg aggtcagtac cggatcgttc gttacgttca ggctgacggg gatgttggtg
     3361 atatctccac cggcacccgt cacggcaacg gttccggaat acgtacctgg tagcagccct
     3421 gataccacgg gaccattcag gccggccaat atgcttcccg gggtggtggc tgtcgcggtg
     3481 gtcagtgtaa gccactggcc gccggatgca ggggtgaaag ctgaactgaa ccccagagga
     3541 gtggaggggg tggtgctggt aacggtaacg gtttgggccg gcggaagctc tcctccggac
     3601 tgatacgtaa aactgagcga ggcgggcgac gtattgagca acggggagtt gctgacgcgc
     3661 agtgttacag tgacgttctg agtggggtta ctcgctcccg gggcgctaat cgtcagcgtc
     3721 tcgctgtagt tgcccggggg cagtcccgcc gggttcacgc tgagcgaaag ttcggccggt
     3781 gtgaatccgt tcaccgtcgt cagcacgagc catgtcgccg gaccacgcac gatgctgaaa
     3841 ggaacctgtg tgtccgtgct gctggtgaag gaaatggtct gcggcaccgg ggcggttgct
     3901 ccgatcccgc tctggtagtt gaacagcagg gaactgcggc tgacgttcaa ctgcggagca
     3961 ccgccgatgg tgaacgttac cggaatctga agcgggacgg cgtccggtat ggtctgcggt
     4021 gtcacggtaa tcgtcgcgtt gtaagcgcct tcgttcaaat cctgcggcga cacaccggct
     4081 accagcaggt tcgcggtgga ggtgttcccg ttcgccggat tcacggtgag ccagggcgcg
     4141 gacgacgcgg ctgtaaagct cagcggaaac ccggtgaggc ttgcaatggt taaagactgc
     4201 gtctgcggaa tgactggtga gtctgcctga taggagaaag caaggcccgg ctggctcgcg
     4261 gtcaattggg agccgaagcc cacattgagt tccacgatca gcaactgttc gatagcaccc
     4321 tgtacgcgaa taaacagtcc gagcgtgtgg cggctgccgg gggtcagtag ggcggtgttt
     4381 ggcgtcaccg cgatcgtggc cggtgtcacg ccggacagcg ttgaaaccga caatagctcc
     4441 gagctgttcc cgatgctaaa agaaaagccc gccggattgg tggtgacggt gaatgtctgc
     4501 ggttggggga tggagccggc cgcgacgttg aaagtcagct tggagggcga gacctgaaca
     4561 gtctgcccaa aagcggccgt accgaaaaaa caggccaagg cgatagccgc aagacgtatc
     4621 acagtagtcc tccaggggat aaaactgcca tgtggggatg atcccggccc ctgatagagt
     4681 gcacgtagtc cgccaccaca gattgtgtgt cttttgtgcc agatgaaacg gcgggcggcc
     4741 cgccgtctgt ttcggaatgc cgcgttgagt gtggctattt ggattgctcc gcggttcagg
     4801 acgctaccgc ttgcgacgcg gcatcgaggc ggcaccggcg gacagcggat ttccagccgc
     4861 tatacagttc ttcgcgccgc tccacctgca tgcgcggctc gaagacgcgc tggggctgcc
     4921 aaaactgttc gagttcgccg ctatctttcc agaaaccggc agccaaaccc gccaggaaac
     4981 tcgcccccag cgccgtcgtc tccgtatcga cggggcgcac cagcctccgt ccaaggatgt
     5041 cggcctggaa ctgcatcagg aaattgtttg cggccgctcc tccgtcgaca cgcagctcgg
     5101 acagtgactc ccgggaatcc gcttccatcg cttccaccag ctcgcgggtt tgataggcga
     5161 tgctttcgag tgccgcccgc acgatgtgct gtgcgctggt agcttgcgta atgccgcaca
     5221 cgactccgcg cgcggaggca tcccaatgcg gcgctcccaa gccgacaaat gcgggcacaa
     5281 gatacacgcc gtccgtatcg ggcacggagg cggccacggt ttccgactcg gcggcggtcc
     5341 ggatcaggcc caatttgtcg cggagccatt gaatggccgc tcccgcgatg aagatgctgc
     5401 cttcgatggc atacgcggga gagtcgcccg aaccggctgc tcgcgtggcg acaagctggt
     5461 ttccggatac gggccggtgc gcgccggtgt gcatcagtgc gaaacaaccg gttccgtatg
     5521 tgtttttgca caaccccggc cggaagcagg cttgtccgaa caaagccgcc tgctgatcgc
     5581 cggcaatgcc gccgatgggg acttccgctc cgaggtgttc cgtggcggcg acgcctacga
     5641 ccccgctgga gggtacgatt cggggcagca tggcgcgcgg cacgtcgaag taatcgagaa
     5701 gctcgcgatc ccagtctccg gattcgagat tcatgagcat ggtccgcgaa gcattcgacg
     5761 tgtctgtcac gtgcacagcg ccattcgtca gtctccagat gagccaggtg tcgacagtac
     5821 cgaagaggat ctccccatcc cgggccttgg cgtacgcgtc cggtacggca cgcagaatcc
     5881 attgcagctt ggtcgcggag aagtacgcat cgatcaccag acccgtcttg gctgaaatcg
     5941 ctgcggcact cccggaacgc cggatctcgt cgcactcggc cgcggtgcgg cggcattgcc
     6001 acacaatggc aggagccacc ggctggcccg ttatcctgtc ccagacgatc gtcgtctcgc
     6061 gctggttggt gatgcccagc gccaccaggg cggagggtgc aacggaggcg gtctccagaa
     6121 cacggcgcgc cgactcgatt tgtgacaccc aaatatcctc cgcgtcctgc tccacccatc
     6181 cggattgcgg atagcggcac gggatgggcg cggattcgat ggcgatgcgg cgcgcttcac
     6241 ggtcgtacaa cgccgcgcgc gcactcgtag tcccttcgtc gagagcaagg atgtaaggca
     6301 cctccgcatt atagaattga gatatgttct tccgccgtga gcagccgcag cagttgacct
     6361 tcgacgaacg aatcggaagg gcgcagaaag ccggtttttc cgcggagaaa catccggacg
     6421 ggcgtgtccg gctgagcaag gacggctgcg ccgccatagt ggaagacgtt ccgggtccac
     6481 ggcctctggt ggagcgtgcg ggcattttga tcggagacga aatcgggaag ctcgtacatg
     6541 gcggctatca ggaatttctc ctgacaccca gcggacgaaa actgccggcg ctggccaggc
     6601 acctgcgggt gttgcacgca ttccaggagg atctgcgcga agccctcggg cttgtcagct
     6661 tgtataacga gtctctcggc acggtgaacg attcccacat gtacgaccgc cttgaagaca
     6721 gggaccgcgg tggacggcct cgcccatggg aacagagaat cgtgtcctga tagcgcaaac
     6781 ggcctacttt ccggatgcgg taatgctata gttggatagc ctcgcgtgaa acagaaagcg
     6841 tttctcattc cgctgctgct agcccaggct tgcctgggtg catcaggagt agtgggcgta
     6901 acggttcacg gagtggttca ccccatcact acggagatcg tcggccacgc catcgagcag
     6961 gcgaaggtcc aggatgccgc actgctgctc atccggatca atactcccgg cggcctgatg
     7021 gaggcgaccc gggagatcac cgagaagatc gttacctcgc cgatccccgt tgtcacctac
     7081 gttgctccaa gcggtgcgcg tgccgcgtcc gccggcttct ttattctcga atcgggtgat
     7141 gtggccgcca tggcgccggg aacgaacaca ggcgccgcct caccgattgt catgggccgc
     7201 gaaatggacc cggtgatgcg gaagaaggtg gaaaacgatg ccgccgcgca actgcgcact
     7261 ctcgccggcc ggcgcggacg gaacagcgaa ctggcggaaa aagcggtatt cgaagccaag
     7321 tcctttaccg aggaggaagc tttgaagtcg caactgattg agctgattgc caccgatgac
     7381 cgggatctgc ttcaaaagct cgatgggcgc gagatcgtga gattcagcgg cgcgcgcagc
     7441 acgcttcagg tcgcttccgc cgagatcacc gagtaccagc ccagtctgcg cgaacgggtg
     7501 atgctggcgc tggcgaaccc gaacacggcg ttcatcctcc tcatcctggg cgcgctggga
     7561 atctatgtcg agttctcctc gccagggctg atctttcccg gggtgctcgg ggggattctg
     7621 gcaattctcg gtctttccgc cctttcggtg ctgcccataa actgggctgg agccgcattg
     7681 atcgttctgg ccctggcgct gtttgtcctc gaagccaacg tcacctcgca cggcattctc
     7741 ggcatcggcg gtgcggttgc aatggtcctg ggggcgctgc ttctgatcga aggtcctccg
     7801 gagatgagaa tcaacctgac cacggccatc gcagttacac tgcctttcgc gctgattacc
     7861 atttttcttg tgagcctggt gatgcgggcc cgcaaggcga aagttgtaac cggggatatc
     7921 ggtatggtcg gagaggccgg ccatgcactg actccgctat ctccgggagg aaaggtgttc
     7981 gttcgcggag aatattggga cgctatcgca actgttccag ttgaagcagg cgcccaggtt
     8041 attgtcacag cggtaaaagg actgaggctc acagtggagc cgttcaggta gagccatgtt
     8101 tcagactccc atgcttgatg ccacaaccat cgtactgatc atcatcgcaa tttacctgct
     8161 gtcttctatc aagatactcg ccgagtacga acgcggagta gtcttccgcc ttggacgggt
     8221 gctggcgcag ccgaagggcc ccggaatcat cctggttttt gcacctatcg accgcatggt
     8281 gcgcacttcc ttgcgcattg aaacgctgga ggttccatca caggacgtgg tcacgcgcga
     8341 caacgtgacg gtgaaggtga acgcggtcat ctatttccgt gtgctcgatc cgaacaaggc
     8401 agtactcgag gtgacgaact acctttacgc tacttcacag ctcgcacaga ccacgctgcg
     8461 aagcgtcctg ggagaggtgg aactggatga gttgcttagc cagcgcgaga agctgaatgt
     8521 gcggctgcaa tccgtgctcg accagcatac cggtccgtgg ggcgtcaagg tgagcatggt
     8581 tgaggtgaag caggtcgacc tgccggaaat catgattcga gccatcgccc ggcaggcgga
     8641 agcggaacgc gagcgccgcg ccaaaatcat ccatgcggaa ggcgagtacc tggcggcgga
     8701 gaaactggcc atggcggctg agctgatcca gcaccaaccc gcagccatca cgctgcggta
     8761 tctccagacg ctggtggaaa tagggtctga gaaaaatacc acggtcgttt ttccattgcc
     8821 catcgacatc attcccatcc tgcgaaaagc gatcacggag actgttcagc ccaagcagta
     8881 aaccatgccg aggaacgaag aaggcgagtt cgaattagtg ctgggaaaca aacagttgct
     8941 cagcttgttt ttcatcgtgg tggttctgct gggcgtgttt ttcacgatgg gctacatcgt
     9001 cggcaggagc tcctcccccg ggccggatct ggcgagtgcg ccggaagcgg tatccagcgc
     9061 gcctccggcc gaagcgccgc gagagagtcc ggcggaggcg gcgtacgagc ctccatccca
     9121 gcgcaccgtg gaggtccgcg aggcggcagc gccgcccacc cccgccccca ccccgccccc
     9181 cagggagcgg cccctgtttg tggagccggc cgctggcgaa acctatttgc aggttatggc
     9241 ggtagcgcgg cccgatgccg agctggttgc cgaggccctg cgcaagaagg gacttgccgc
     9301 cgccgtggcg gccggtccca gcccggcgtt ttaccgcgtc ctcgtgggcc cgttgagcga
     9361 caccgcgtcc attgcctcgg ctcgcgccga aatcgaaggg gcggggttca aaaaccctat
     9421 cgtgcgaaaa tattgaggtg ccgggagacc tcgtccacat tcatacaggc cacgcccgcc
     9481 tgccgcactg gctgcgaaaa ccggaaaccc attttgaatc cgttcagcat ttgaaacgca
     9541 acctgcgccg cctgaacctc aacacggttt gtgaatcggc gcgctgcccg aacatgcacg
     9601 agtgcttcca ccgcggcgcg gcgaccttca tgattctcgg aaacctctgc acgcgcggct
     9661 gcgggttctg ctccgtgcca aaggggtcgg ccgcgaagcg cgaattcttt ctcgatcctg
     9721 gcgagccggc gaatgtggcg agaatggcgg aggccatgcg cctgcgttat gtcgtcatca
     9781 ccagcgtgaa tcgcgacgat ctccccgatg gcggctcgcg gcattttgcc gaaaccgtcc
     9841 gggaagtgcg ccgtgcactg cccgaggcgc aggtcgaagt gttaacgccg gatttttgcg
     9901 gagacctgga agcggccggg cgggtgctcg atgcctctcc ccatgtgttt aaccacaata
     9961 tggaaacagt ggctgcgctg tatcgcaagg tgcgtccgca ggcacgatac gatcagtctc
    10021 tcgcggtgtt gcggtatgcg gcgaagcaca acccggctat cctcaggaaa tccggcctga
    10081 tggtgggatt gggcgaaacc gccggacaag ttcacgagtt gttgcgtgac ctgaacgagg
    10141 ccggtgtgga tgtggttacc atcggacaat acctccagcc cacacggcgc aacctgcccg
    10201 tcgcgaagtt cgtgacgccg gaagagtttg acgcctatcg cgattatggc ctgtcattgg
    10261 gtttcaaaat ggtcttcagc gggccgttcg tccggagttc ctacatggcc gatctggtca
    10321 acacccgggc gcgcgagtgt tgaacttcct gtttgcagcg gtcacggcaa tcctgctcat
    10381 cctcaccgtt ccgaggtttg agattggctg gctcgcccct gtcgcactgg ttcccctgat
    10441 cttcgcgctt gcgcgggagc cccgctggcg ttggcgtttt ttattgggct ggctggccgg
    10501 cgtcctctac tggttcggag tctgttactg gatacagggc gttttgtatc agtacggcgg
    10561 tttggggcag tttgccgcgt gggcgtcttt cctgctgttc tgcgcggcca aggctctgca
    10621 cctggccgtg ttctcggcgc ttgcgggcta cgtcctgcgg gcatggtacg ccatcccggc
    10681 cgtagcggcg ttatggaccg gcattgaacg tacccatggt cccctgggat tcacgtggta
    10741 cctgcttggg aactcagccg tcgacatgga ggcgcccatg cgtctcgcgc cctacgtagg
    10801 catctacggg ctgtccttca tccttgtgat gatgaacgtg gccgtcgtac tggtgatgct
    10861 gagaaggccc cgccgggaga ttctctggct ggtttcgctt ccgcttctct acctgcttcc
    10921 gaacatgccg ctcccggaga agccggaacg ggtggcggct gttgttcagc ccaacatcgc
    10981 ggaagagacc cgttggacgc cggagagcgc cgatgcgatg cagcgcagct tcgagttgct
    11041 gtcactacag gcggccttga aaggggacga gcccccagta gatctggtga tctggccgga
    11101 ggtgcccgcg ccgttgtact acttcgaaga ccccgtgctc cggaagaggg tagcgggcgt
    11161 ggcgcgtctg gccgatgcgc atgtgctaat gggcaccgtg gcgcacgcac cggacgcctc
    11221 gcccctgaac tcggcggtga tggtgaagcc cgatggagag gcggaaggca gatacgacaa
    11281 gatcttcctg gtgcccttcg gggagtttgt accgcctctt ttcggattca tcaatcagat
    11341 caccaccgag gcgggggagt tcaagccggg cgagaaggtg gtcacgtttc cggtaaacgg
    11401 gcagggcgtg gggcccttca tctgctacga atcggtgttt ccgcacctgg tccggaagat
    11461 caccggcaac ggcgcgcatc tgctggtcaa cttgtccaac gatggatatt tcgcccggag
    11521 cgccgcccgc gagcagcatt tgaacatcgt gagaatgcgc gcggcggaaa atcgccgctg
    11581 gattattcgt gccaccaacg acggcatcac cgtcagcatc gatcctgccg gcagaatacg
    11641 tcagcggctt ccggctcatg agcggctgtc tggccgctta agctactcct atttgagcga
    11701 tgtgacgttc tatacgcagt atggcgactg gttcgcctgg tcctgtctgg ccgcggggct
    11761 tcttctcgtg ctggtatcgc agattcccgt gtaccgtccg gaggagccgc gccgctagga
    11821 ccgcttgctc gcgcgcgcgg ctcagtaacg cgacggtcac ggtgcggggg tcagccgcaa
    11881 atctcccggt aatactcctc gtaggccgga atgatcaaag atgtacagaa acgcgtgagc
    11941 gcggtgagcc gcgcagcggc gcccatcttc ccgagcaggt cccggtcttt cagtaaggaa
    12001 acaactcgcg cggcctgagc actaacatcg cccacgtctt ccaggaaccc gtcggtaccg
    12061 tgggtcacaa gctcgggcaa tccgccggcg cgggtagcca ccggtggaac accgcacgcc
    12121 attccttcga gcgcggcgag accgaacgat tcgagctcgc tcgggagaag caagacatgc
    12181 gcattgggaa agaggcgttc cacgtgagcc tgcttgccga gaaaactcac gttgttttcc
    12241 acgccgagtt ccctggccag ccgttccgct ggcccacgct ccggtccatc cccaaccatc
    12301 agcaggtgtg cgggagtctc ctggatgacc tcccgtagaa tccgaacgca gtcgagagga
    12361 cgcttcaccg gccggaagtt cgagacatgc atcaggatgg cggcgcccgg tggggcgagt
    12421 tccgaagcga gcttgcgctc cggatcaggc cggtacagct cgcaattaac gaagttcttg
    12481 atgacgcgga tgtctttctc cactccaaaa acttcaatgg ttcgggtgcg caggtagtcg
    12541 ctgatggcag tgatgccgtc ggatttttcg atcgaaaact tcgtgatggg gaaatacgag
    12601 cggtcggcgc ccaccagcgt gatgtctgtg ccgtgcagtg tggtgatgaa gggcaggcgc
    12661 cggtgcgatg gcatttgcga gaccatcatg tcgcgcgcca gcagcgccga aatcgagtgc
    12721 ggtatcgcgt aatgcacgtg caggagatcc agcttatagc tttccgccac ctctgccatg
    12781 cgggaagcca gggcgaggca ataaggcggg tattcaaata gcgggtaggt cgtgacttct
    12841 acttcgtgat agtggatgcg gggagtcccc gagtccagcc ggatcgggtt cgcatagctg
    12901 atgaaatgaa tctcgtgccc gcgataggca agctccatgc cgagttccgt tgcgacaatg
    12961 ccgctgcctc cgtaagtcgg gtaacacgtg attccgattt tcatctactt cgtcaggcta
    13021 gcatcttgaa accgcagcat gccgattcct atcgcgccgt tcaggttttc gtccggactc
    13081 ggacgatccc cgccgccgaa ccagacccgg atccggtcgc cgagcacctc cacatcgggg
    13141 tcgcacagca cggcctgatt ccaggcagcc tcaccggaaa aaaccagatc gcggcgcttg
    13201 ttccatctca cgccgtcccg cgattgcgcc atgcccaggc ggcggatctc cgagcggtcc
    13261 cggcccgtgt aaatcatcca gtagaaaccg tgagactccc acacggcggg ttctcccagt
    13321 ccgttatcgt cgaactcgcc cggctcgccg ggttccagga cggggttgga tctcaacttg
    13381 gtccagcgta ctccatccct ggaccgggca atacccaggc gctgccgccg ggcgcgatcc
    13441 atgccgagat agtacatgaa gaagaatccg tcccgccgga ggacataggg atcgccgatc
    13501 gaaatttcat cccaactgcc tcgtggtcct gccggcagga caggctgcgc cacgcgcgac
    13561 cacgaagcga gatcgcgaga ccgtgccagc ccaatgcggt tggcgttacg cggccccgcc
    13621 tgataccagt agaaccattc gccctcatgc cacaacgcgg agccatttgc ggagatataa
    13681 tccgattccc aggcattcgg accgggcgac agcagcttgc ctcgccgtag ccaaccggag
    13741 ccgtccgggg aaatggcaag ccccgtatgc cacgtctttc catcgaaacc cgaatagaaa
    13801 ttaaagtatt ggttgacatg gcgcacgatg gaaggattca acacgtccgc cgagtcccaa
    13861 cgtcccgccg gcccgcgttc gagaaccggc aaggcttcct gttcgaagag gaatctcccc
    13921 tgctgcggtt cacttgtggg ttgcgaaagc gcaaactcgc cgtagctgcc gcacccgctc
    13981 gcaagcagca gggcggcggc aagcccggcg cgtaggccat cagccatgcg gcacgcggat
    14041 cctcatggaa gctcccgaac cggggcgcga atcgagcgtg agatcgccgc ccaggctccg
    14101 gatcctgcgt tttatgctgt cgggtcccag ccgcaacaat tccagctctt caagggagta
    14161 gttgccgctg aaagtgaagc cggtgccgtc gtcctcaatg acaacgtcca acgcgctgtc
    14221 gccgtgtgag acggataccg ctacgcggga agctttcgaa tgcttctgca cgttattcaa
    14281 agcttcacga acgatggaaa gcacctcggt cgagagttcg ggttcctcgc cggagaccga
    14341 ggcggtgtca tgaaaactcg ccgtcatgcc cgagtccttc tggaatcgct cgaccagatg
    14401 gcggatggaa gtgatcatgc cggcgccttc gatttcggcc gggcgcatac tgcgaataaa
    14461 ggacctgagc tcaacgacct ggctgtggca gagttcctga agttgccgca gttcgtccat
    14521 gccgcgctga aaatcgcgca ttagcatctt gcggacgatc tcgagtctca tctgaacact
    14581 gatgaagctc tgaagcgggc cgtcgtgaaa gtcggccgcg atacgctgcc gctcctcttc
    14641 ccgggacttt tcggcttccg cccggagaat caccgattgg cgcgcagtgg aggagagccg
    14701 gttctgaagt atttcgcgat gcagcgtcag gaccaggccc acggtgcctg cacagagcat
    14761 catcagccag ataccggatg cgccgatcgg ccgcgcagcg cctaaaaaca ctgccagcac
    14821 gcccagcagg aagctcaatt cgtaccagga gtgcagaaga ctgatccgca gcacgatata
    14881 gacgaaaaac gccgcggtga caaagaggaa gcgccccgcc gccgcataag tccagatgag
    14941 gaaaatgccg aaatcggcga acaggatcag cagaggttgg gcagcacggt ctgccgtccg
    15001 ccagaacagc gtggcaatcg cacccgccag gtatagcgcc agcagaaagc ttagtccgga
    15061 aaagcgctcg gaccactgga tctgaaaaac aaggcaggaa agggcgataa aaatgcgcgt
    15121 caccgcgaga gcgcggcgcc acgtgtgggg aactagtatg atgcccgctg ccaagctcgc
    15181 gttccgttca ctcttgatcc gagtggcaag ctcatctcac atacagatac agcgtatggc
    15241 tgcgcccttc gccatcgtcc tcaatatgct ccgtcttcac cggcgtccaa ccggtgaatt
    15301 caaagtcgtg tgaaaccacg cgggcgcctt tcttgagctg cttttcgagc atgggccgga
    15361 tcttaacgtt cgacgcgggc agaagataca cagtcaccag gtccgcgtcc gagtaattct
    15421 ggcgtgtgat gtcgccgtta atcacccgcg cgtttttcaa gccgaggccg cggattctat
    15481 ccatactctg tttccaaagg tcttggtcca tctccacgcc ggtggcattc gctcgaaatt
    15541 tccgggcggc catgatcacg atccgcccgt ctccactacc tagatcgtac attctttcgc
    15601 cctgcttcaa accgccgaac tcgagcatct tctctacaac cgtctccggc gtaggatagt
    15661 acggcgccag tttctcggca tccgccgtct gctgcgagag cgcgatgtag ggcagtagaa
    15721 atagaaccgg tatagaacga atcatgatgc tccgccgtaa agtagtctac ctcaataatc
    15781 cggtacggat ccccgcgcga cgctttacag ttcagacggg cgccggcgat taaggttcca
    15841 gataaacccg tactctatta ctttgttggc cctcggcctc cagatacaat tcggccgggc
    15901 cgctgttcac gatagccggt actctgatct caaccaggta catgccgata taacccggag
    15961 ccagaattgc acgagtcact tcgaccgggc tgccatccag aaaggcccgt acaggcaccg
    16021 ctacggccgg tgagttttcg agcggcgccg gtaatcccgt tggccactcc ggttctacct
    16081 ttcccaggcc cgcggcgaga atctgaaggg ccgtgccgga tcgtgccggc gtcgtcgcgc
    16141 tcagcgccag gccgttttcc ccatccaaca ggaatggcgt cccgtccggg tcgacaaaaa
    16201 tcgccggtga aatcttttga agcggtaaac ccagggtata ccggccgcct gtgccctgca
    16261 tggcgagcgt gatcgaggtc ccggtgacct caaacggaat ttgcatctgc gtggcaccgg
    16321 ccgaagcatc cagcacggga accgggagcg ctcccgcacg ggcggattga atttgcgcgc
    16381 cgatcatgct gatcaaccct cccggtgccg cagcgcgaat gttcaggtcc gcagcattga
    16441 cgagccgggg ttcggaggcg cgatgcggcg ccggcgccgc aaacaggccg tatccctcta
    16501 ttgcggcata gatctgattt ccggccgcat ccaggcgaac atccctcacc gccgcttccg
    16561 gcagtttccc gctcacttcc gtccactgtg tggcgggccc aagagcgccg aggtccgtac
    16621 gggtcatgaa cacgccgtcg ctggtgccgg cgtaaatggc tccgctcgtc cggtcggcag
    16681 tgatcgaata caccgccgct ccggacaggt ttgcggtgat atcgtcccaa aacgctcccg
    16741 cgttgaatgt cttgacgatc cgcgctgttg attgggcggt gccgcctgcg cttgtgggcg
    16801 gtccgaagac ggccagtgcg atgcgggcat cggaagggtc gatccagatc tgttccaccg
    16861 ggccatccga ttgctcctgg cgcggaaacc atgtacgccc ctggtcggaa gatgcccaga
    16921 gccggccgtc ccccgcaccg gcataaacaa actcccgtgc cgacgctacc gccgtgattc
    16981 gtgcgccgag cagtacggaa tattcgcttt tctggacgcg ctcgcgcgcc agcgtgtcag
    17041 acgcgatggt gggccgccat gcgatttttt ctcccggcgc ccattcaaac tgcgccggcg
    17101 cggcgcccgt gccggattcc gccagttcga tcgtcatccc gtccacgcct ttaggtaacc
    17161 gcagaatgcg ctgaacaggc aggttgggca ggcccgtgtt gatgccgccc caggattctc
    17221 cgccatccga ggaacgccac aggccataat cgccggcaac cacaatttcc gaggcattaa
    17281 agggggaaat cgccaaatcc cgaatcgggc tcccgagaag ggacaccccg tggtacgccg
    17341 ttaaatttct ccatgtcgcc cctccatcat cggagcgcca cgcgtgctcg ccgaaagcgt
    17401 agagccgtgc ccgggcgtat cggatctgcc ggacttgcgc tcccggctcc ggcaaacgga
    17461 tgacggttgg cggctccggg gcgggaggga gaggttgaga cggttccgtc caacgcttca
    17521 ggtcttcggt ctcaaattgc cggcccgccg ccgtccggac gtacaaacgt gcgccatttt
    17581 ccgcaaacca gacgcgctcg acaccgccgg atgccggtgc ggggagattc aggtccagta
    17641 cggtgttgcc gatgctgcgc cagtccggcc ggagaccttg agccggcagt atattcgtga
    17701 caaagagtgc aataagtatg gcgtttcggc tcacaactgt ggctttccga cccatccatt
    17761 ttgacacgat tctacctcat accccttgtt ccatattggg aagtgccgat tcgccggtca
    17821 tttgcgcatt aattctttac aaggcttgct acttgctaag caatcttggt gtaaactaag
    17881 gatctgcggc cgcatttaga cctcagccag aactttgcaa tggcgcgtct gtcaacgtgg
    17941 ttcgaggtag gacttgccgc ggcgttactt cttcccgccg ccttctccca aattgtacca
    18001 ggacgagccg accaggctgc gcgtgttgta tcgctcgacg gacaggtctc tgtcatgcgt
    18061 gatgcgatac cctgggcgct gaaagaaggc gatccggtac gggttcgcga actcattgtg
    18121 accgggccgg atggctccgc tgtctttgaa ttggccgacg gcagtacgtt tgaaatttat
    18181 ccgaattccc acgttacatt tcgaaacact cccgggaact tacgcgactt gctggatttg
    18241 tggctgggcc ggatcaaggt tcatatccag aagtttggcg gccagccgaa tctgaaccgc
    18301 gtacacactc cgactgccgt catttccgtg cgcggcacgg ttttcgaagt gatggtggac
    18361 gaagaggaca agacgacctt cgtcatggtg gaagaagggc aagtggcggt gcagcacgcg
    18421 cttcttccga gcggcgagcc gaaaatcctg aacgccggtg aatacatcca ggttcacaag
    18481 gatgctccgt tggcccggcg gggtgtggac cgggacacgg tgttcaaaca tgcattccgc
    18541 gcactgattg atgccatgta taccgctgtt taccggacgc cccggagtgg cggtggtgtc
    18601 tcagtccccg gcagcggagg cggcccgcag attcctggcg acacgggcgc cacacccgcg
    18661 cccgagagcg tgcctggtcc aggggatgtt ggtgccaccc ctccacctcc ggccccgcct
    18721 gcttcggcac cgtaaactca ctcgggatgc gcgcttggga tgcgcgtcgt cgaagtactc
    18781 tgaaggggac ctcatgtgtg ctatacgtct gctggccgtg atggcgattt cgtcggctct
    18841 ggccgcaggt gacggttcat ttaccgtcgc tggtctgcgg ctgatgccga agcgctggga
    18901 taaacaagcc aatttcgaaa agctgagcta ttacgcacgc aaggcggctg aacggggcgc
    18961 ggaagttgtc gtcaccccgg aagggtttct cgagggctat gtcgggaatg aaaaggccaa
    19021 taaagacctg acgcgcgacc ggtacttcgc catcggcgaa ccagttgacg gcgagatgat
    19081 cggacgctgc cgtaatctgg ccaaagaatt ggggatctat ctggtcctgg gctttgcgga
    19141 acgaagcgga gaccagatgc ggaacagcgc cgccgtgatt tcaccaggcg gcgaaatcgt
    19201 cctgaaatac tcaaaaagcc acacggccga tgatgagccg ttcaacacga agggcaggga
    19261 atttcccgtt acagacacgc ctttcgggcg ctggggcacc ctgatctgca tggaccggca
    19321 gttgcccgaa acctcgcgca tcctcgccgt caaaggagcg caaatcatcc tcgttcccgc
    19381 atggggcatg aataatgaga ccaacgatat catgatgcgc acgcgcgctt atgaaaacgg
    19441 tgtgtgggtg gtgttcgtgc atccggagcg cgtcctggtg atcgacccgt ccgggaaaat
    19501 cacggccgag gatagccgtg agggagacga agcggtactt gcggaaatcc gcgtcgacag
    19561 ccgtatcggc cgtggaccga tacggcaccg ccggcccgag atctacggcg agattctagg
    19621 gcaggcgaag tagcgcgccc gcagtgagag ctgtgctcag catggcaata tccgctacgc
    19681 ccgatgaatc gctttcaccg ccagctgtgg ttcgtgtgca atgatcttca acaaagcggc
    19741 cgccggtccg gtggggcggc gtcggtcctg ttcccagttc tgaagggtct tcacgctgac
    19801 gccaatcaag gaggcaaact ccatctgcga gagcttggtt ttttcgcgaa tggtccgcac
    19861 accagacgcg ccgattacaa cgcgccgcga aggcttcttg gattgattcc ccatgttgag
    19921 tttggcaacc gggtctcagg cgttggatgt cacggggcga cgatgggtca ggaaaagaat
    19981 ccctgccgcg aggggtggtg caccaaagac aagagtcgtg agtaggcgtg acggccacca
    20041 gatcgcatga gcaactccag ccgacaatcc gacggcgata atcagaagtc ctccgatcat
    20101 ctcccagcgc cacggtacga cagccgaaaa aacaaaaagc aggcccacac ccacccaggg
    20161 cagcgccgag gacacaggcg tgcgccacac caccgactct actacaaaaa agaacatcca
    20221 aaatacggcc caaaacaacg tcagcgcgcg agccgccagc accaacggac gtttcatacg
    20281 agcctccgag taaagtatgg atccggcaca cacgcatagc aatccagtcg tcgggccgcc
    20341 cgcagtgaga caaatgaaac agtgatactc ccgcgataca atcaaggtaa tggccgaaaa
    20401 gctgctaacg gtcaacgagg cgatccgggc gttaggcgtc tcgcgttcga cgctttggcg
    20461 gcggctccag agcggggcca tccccagcgt caggcgcggc ggacgccgtc ttgtgtgcct
    20521 gacgatgacc cggaaggcca cccgtgggga gattgcgggt gatatcccgc ccttcactgg
    20581 aaagcacccc atcttccgcc tgataggcgc agggcgcagc ggcggccgaa caccaggggc
    20641 gcgcgacaaa cacgcgattc tggacgcatg agcatcaagc ggggcgttct ttgggattcg
    20701 agcgcaattc tcgcgctgct ggatgcggac gatcccgatc atgagctcgc agttgcgatt
    20761 gctggccaaa ttgcgtccca gcggcggcca agcttcatca cgaactacat cgaagtggaa
    20821 gctcatgcgt tgttgctacg caagttggga acggcgctcg cccgagagtg gctactcacc
    20881 ggcggtctgc cggtgatccg ggcattaccg gaagaggagg atcgcggccg ggagatcgtc
    20941 gcgcggcatt cggacaaaga ctggagtctt tgtgccgcga tttccttcgc gattctggat
    21001 gtgcggggaa tccgccgcgc tttcacgttc gatcaccatt tccttcaata cggccggatc
    21061 gaggtcctcg gtctatcgcg ctgattcggc gtagctggcc acttggcagt atcacgcctg
    21121 ttgccctctt gtacggactc ctgaaaccgc agatagcgca aatcaacgta acggggacaa
    21181 cacgtccagt tgggaagacc tctccgaagc agacttgttc gctatctcga ctgaatcgag
    21241 aggcaacgat cttaagaagt gatatacgtc ggaagtggca gggcggtgag gctgcagaag
    21301 gcgcgtatcg cacacttgag cgcgtaggag ccaagttgca gccggtaaaa gtccgtgcat
    21361 gagctgctca acagtacatt gatcgaccat gctgtcggat gaaccatttt agagcagagc
    21421 ttgaaccaga acttgtcatg agtcccagtc ttcgccagcc gcatactctt gccggagact
    21481 tcccattgct tgacccttct ttccagcgac gagtattcgg gatcggatga gtccacgagc
    21541 ccaaacatct ccacgacatc aacgaggacg tccttgtcga accgctgcat gttctcattg
    21601 ttttttgtga taaactcgca ccacaaagca acctcgatca ggtttctcgt caccagagcc
    21661 atcaggtcaa tcggaacagg cttccgggcc aatatccggc caagctgcac atcgatatag
    21721 gtaaggagat tccgaaagat tcggtcctgc ttcgtgttgg cggacaattt ggccctcctc
    21781 aaaaccgctc ggataatacg ccggaacctt cttacaacat ccttcaggcc ctgaatcgtc
    21841 ttcggcggct gtaattcaag gttcgacatt gtcagaagtc tagcatcatt ggcaggtcac
    21901 ttggccgtaa aacgcctgtt gctggcattt tgacaggttg tggtttacgc caacgcggat
    21961 tcgagactga cgtgcggggt tttgccgcga atatgcgggg tatcctctac aacccggttt
    22021 ccagcgaaaa tctcgttccg tccgagcgga tcgtgatcgc tcccgaataa tccgtcctca
    22081 agactctggt cttgtccgct tccaaacgct cgatcacctc ggggtggggg ttacggtacg
    22141 aattttcgaa tccaacggag atgattccga atgcgggtct cagagcttga aggaactcgc
    22201 tgccggtgga cgtcctgctg ccgtgatgcg ctacctttag tacatccgtt ttctcaacgg
    22261 gaagcgcata ctccatccgg cgctccaggt ctcccgtcaa cagaaacgag tgccgcgcgt
    22321 aggtgaatcg caggacaagg gaatcgttgt tccccggttg ctcacccggt atgtaatctt
    22381 ccggaggcga aagcacctcg acacccgcgc cgccgaagga aaaccgcgtg cccgcccgga
    22441 gcggcttcac ctgaatgccg agctgcgacg ccttcgcgcg aatagacctc cacaagtcaa
    22501 tgtcgggcgt ggctcccgtc cacagctcgc gagggcggaa attttccatg atcgcctgga
    22561 tcccgccaca atggtcggcg tgcaggtgcg tgaaggcgat tgcatccagc cgccgtatcg
    22621 atctcttcca gagataagga gagactacat cttcaccgat atccaggcgc ggtgcgctgc
    22681 ggcccccgaa cgtggggatc ccgccgccgt ccaccatcat cgttgttcca tcgggcaagg
    22741 cgagaaaaag gctgtccccc tgcccgacgt cgatagcagt caattcgaag gagccaggaa
    22801 cgacgtccgg cttaaacggg tgccatatga tcactacgag cagaatgcct gcccccatca
    22861 tcgcgcccca tttccagatc ttcctcgagt gaggcacgaa cgcgacggcg gcgagacaag
    22921 cggcaaaggc gagcgccaca atcaccggcg ggtccggaat tcgccagttc ggttcccatc
    22981 ccgcatgcca gtccacaacc acgcgggacc aatccagcag cagcccggcg gcttgtgccg
    23041 gcagtttcca gccggccagc acggcaagca gcccgagagg acccaccaga gacgtcaacg
    23101 gcacca
//
LOCUS       DASTMK010000020         4258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_257234, whole genome shotgun sequence.
ACCESSION   DASTMK010000020 DASTMK010000000
VERSION     DASTMK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257234"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2682)
                     /locus_tag="VFQ79_00745"
     CDS             complement(<1..2682)
                     /locus_tag="VFQ79_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEU0138200.1"
                     /translation="MSRSVPAGFPERQGLYDPGNEHDACGIGFVVNIKGGRSHEIILK
                     GIQILINLTHRGACGCDPETGDGAGILIQIPHAFFARECASLGFTLPAEGEYGVGMMF
                     LPVELQPRLQCEGIVERIAREEGLTVLGWRDTPVDADAIGRIARASQPYIEQIFLGRG
                     AGMTQDELERKLYVVRKRAEAEIRKSDIRGAEFFYVPSLSSRTIVYKGLLLAPQIANF
                     YGELSDPDTTSALCLVHQRFSTNTFPTWQLAHPFRYLCHNGEINTLKGNVNWMHARHK
                     VLSSPLFDDMKKLLPVIAPGGSDSAGLDNAVELLHLAGRSLPHVMAMLIPEAWDADET
                     MHPGKKAFYEYHASLMEPWDGPAAVAFTDGRVIGATLDRNGLRPARYLVTHDDVLIMA
                     SETGVLPVKPEDVRYKGRLQPGKMLLADTVEGRIIPDEEIKERLYSRQPYRAWIDEHQ
                     ISLAHLPEPPRVYGSDYDTLLQRQRAFGYTEEDLRMILLPMAAKGEEPVGSMGTDTPL
                     ACLSDRPQPLFHYFKQLFAQVTNPPIDPIREELVMSLTSYIGTERNILDETPQHCHTL
                     KLPHPLLTNRDLEKLRRVSQGDFLATTLPMLFRPDGGVEDLERALDGLCRRASLLIKS
                     GYTLLILSDRGVDEDYAPIPSLLALTAVHNHLVREGTRTQVALVVESGEPREVMHYCL
                     LIGYGASAVNPYLAIETLEDMAGKGLLPEGVSFATALKNYRKAINKGLLKVFSKMGIS
                     TLQSYRGAQVFEAIGLNKQLVEKYFTGTASRIEGIGLDVLTREAKLKHEHAFQPLRES
                     ETELDIGGNYHYRARGEYHLLNPLSIAKLQHSVRQNSFNTFKEYTQAINDQSRQLCTL
                     RGLMRFKPERPIPIDEVEPAREIVKRFATGAMSFGSIS"
     gene            2790..3716
                     /locus_tag="VFQ79_00750"
     CDS             2790..3716
                     /locus_tag="VFQ79_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0138201.1"
                     /translation="MNVEHLKLFRDVAQTRSVSRGASLNGVTQSAASQHVQELEKTLE
                     VELLDRSRRPLTLTPAGRLYYDLCRDVLRRHEEFTAALGRLKSDVEGRVRVASIYSVG
                     LSEMSRVEEEYARAFPLAVLEIEYLRPERVLEAIQADRADLGLISYPEPSREIAVIPW
                     REEEMVLAVAASHRLAHRDMVAASEIAGEAFVAFDEDLPIRHEVDRFLREQGVMVDLV
                     MHFDVIQMIKEAVALGSGVSILPRRIMTAEIEQGRLAAVALKAPGLVRPVGIIHRKRK
                     KFNRAAQSFLDLLRNSPEKEPEAVQRAATAKS"
     gene            complement(3731..>4258)
                     /locus_tag="VFQ79_00755"
     CDS             complement(3731..>4258)
                     /locus_tag="VFQ79_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138202.1"
                     /translation="LAARISATSPRTRTVIRFARSLQVRDFHSGNAIILGSRRANPWV
                     ELFESKLNFTVVYDSAAHRIHIANRSPRPGESREYVMESSEAKTGQSYGLISLVPNLS
                     RSGRILILQGLNMEGTEAVGEFALTPQLMGQLLAAIGAAEGEPVPAFEVLIKLQAIGG
                     AAKDTEIIAVRRGRM"
BASE COUNT          831 a         1246 c         1284 g          897 t
ORIGIN      
        1 gctgatggag ccgaacgaca tcgcgcctgt agcgaaacgt ttgacaatct ccctggccgg
       61 ttccacttcg tcgataggaa tcggcctttc cggtttgaac cgcatcaacc cgcgcaacgt
      121 gcagagctgc cggctctggt cattgatggc ctgcgtatat tccttgaagg tgttgaaact
      181 gttctgacgc accgaatgtt gcagcttggc gatgcttagc gggttcagaa ggtgatactc
      241 accgcgcgcg cgatagtgat agttgccgcc gatatcgagt tccgtctcgc tttccctgag
      301 aggctggaat gcatgctcgt gcttcagttt tgcctcacgc gtcagcacgt ccagcccgat
      361 cccttcgatg cgcgatgcgg ttcccgtgaa gtatttctcc accagttgtt tgttcaatcc
      421 gatcgcctcg aacacctgcg cgccgcggta gctttgcaga gtcgagatgc ccattttgga
      481 gaagactttc agcagccctt tattgatcgc cttcctgtaa ttcttcagcg cggtagcgaa
      541 tgatacgccc tcgggcagca gtcctttacc ggccatatct tccagagttt cgattgcaag
      601 atacgggttc accgcgctcg cgccatagcc gatgagcagg cagtagtgca tcacctcgcg
      661 cggctctccg gattccacca ccagtgccac ctgcgtgcgt gttccttcgc ggacgagatg
      721 attgtgaacc gccgtcaacg cgagcaggct tgggatgggc gcgtaatctt catccacgcc
      781 gcggtccgac aaaatcagca gagtataacc ggacttgatg agaagcgaag cgcgccggca
      841 gaggccgtcg agtgcccgct ccaggtcctc tacgcctcca tcgggacgaa acagcatggg
      901 cagcgtggtg gccagaaaat cgccctgcga gacgcgccgg agcttttcga gatcgcgatt
      961 cgtcagcagc gggtgcggca gcttcagcgt gtggcagtgc tgcggcgttt cgtccaggat
     1021 gttccgctcg gtcccgatgt agctggtcaa cgacatcacc agttcctcgc ggatgggatc
     1081 gatgggcgga ttcgtgacct gcgcgaaaag ctgcttgaaa taatggaaca gcggttgggg
     1141 ccggtcggag aggcaggcga gcggcgtatc ggtacccatc gatcccaccg gttcctctcc
     1201 cttcgccgcc atcggaagca gaatcatgcg aaggtcctct tccgtatagc cgaacgcccg
     1261 ctgccgttga agaagcgtgt cgtagtccga cccgtagacg cggggcggct ccggaagatg
     1321 agcgagcgag atctggtgct cgtcgatcca cgcacgatac ggctggcgcg aatagagccg
     1381 ctctttaatc tcttcgtccg ggatgatccg gccttccacc gtatcggcca acagcatctt
     1441 tcccggttgc aggcggcctt tgtagcgcac gtcttcgggc ttcaccggca gcacaccggt
     1501 ttccgaggcc atgatcagca cgtcgtcgtg ggtaaccagg tatcgtgccg ggcgcaagcc
     1561 gttgcggtcg agcgttgcac cgatcacgcg gccatcggtg aaggccaccg ccgccgggcc
     1621 gtcccagggc tccatcagcg aggcgtggta ttcgtagaat gccttcttgc ccggatgcat
     1681 ggtctcgtcc gcatcccagg cttccgggat caacatcgcc atcacgtgcg gcaggctgcg
     1741 tccggccaga tgcagcaact ctacggcatt gtccagcccc gccgaatcgc tgccgcccgg
     1801 cgcaatgaca ggcagcagtt tcttcatgtc gtcgaatagc ggtgacgaca ggactttgtg
     1861 ccgcgcgtgc atccagttca cgttgccctt tagcgtattg atctcgccgt tgtggcacag
     1921 gtagcggaac ggatgcgcca attgccaggt cggaaacgta ttggtagaaa accgctggtg
     1981 caccaggcac aacgcgctgg tcgtgtccgg gtccgacaac tcgccgtaaa agttcgcgat
     2041 ctgcggcgcc agcagcaatc ctttgtagac gatggtgcgc gatgaaagcg acgggacgta
     2101 aaaaaactcg gcgccccgaa tgtccgactt ccggatctcc gcttccgcgc gcttgcgaac
     2161 cacgtagagc tttcgctcca attcatcctg tgtcatgccg gcgccgcggc caagaaaaat
     2221 ctgttcgatg taaggctgtg aggcgcgcgc gatgcgtccg atcgcgtcgg catccacagg
     2281 tgtatccctc cagccgagca ccgtcaatcc ttcttcccgc gcaattcgct cgacgattcc
     2341 ctcgcactga agccggggtt gcaactctac cggcagaaac atcatgccga cgccgtactc
     2401 gccctcggcg ggaagcgtga aacccaggct ggcgcattcc ctggcaaaaa atgcgtgcgg
     2461 aatttgtatg aggatgccgg cgccatcgcc tgtttccgga tcgcagccgc acgcgccgcg
     2521 gtgtgtcagg ttgatgagga tctgtattcc cttgagaatg atttcgtgcg atcgtccgcc
     2581 tttaatgttg acgacaaacc ctataccgca cgcgtcatgc tcgttgcctg gatcgtacaa
     2641 cccctgccgt tctggaaaac cggcgggaac acttctgctc atgtagggct cctgggtaat
     2701 gtagggagta tagaggataa cggcattatc agtcaatcga attattcact tcattctaaa
     2761 ttagaattac ttatgttata gtcgaggtgg tgaatgtcga gcatctaaag ctttttaggg
     2821 acgtagcgca gacgcggagt gtcagccgcg gagcgtcgct gaacggtgtg acgcaatcgg
     2881 ctgccagcca gcatgtgcag gagttggaga aaacgctgga ggtcgaattg ctcgatcgca
     2941 gcaggcgccc gctgacgctc accccggccg ggcggctgta ctacgatctc tgccgcgatg
     3001 tcctccggcg gcatgaagaa ttcacagccg ctttgggcag gcttaagagc gatgtggaag
     3061 ggcgggttcg cgtagcgtcg atctattcgg tcggcttgtc ggagatgtcg cgcgtcgagg
     3121 aggaatacgc gcgcgccttt ccgctggcgg tgctcgaaat cgaatatctg cggccggagc
     3181 gggtcctcga agcgattcag gcggatcgcg cggatctggg ccttatcagc tatcccgaac
     3241 cgtcgcgcga aatcgcggtc attccctggc gggaggagga gatggtgctc gccgtagccg
     3301 catcccacag gctggcacac agggacatgg tggcggcttc ggaaattgcc ggagaagcgt
     3361 tcgttgcttt cgacgaggat ctgcctatcc gccacgaggt ggaccgattc ctgcgcgagc
     3421 agggcgtcat ggtcgacctg gtgatgcact tcgacgtgat ccagatgatc aaggaagcgg
     3481 tggcgctggg atccggtgtt tccatactgc caagacgtat catgacggcc gaaatcgagc
     3541 agggccgtct cgccgccgtc gcactgaaag cccccggact ggtgcgtccc gtcggcatca
     3601 tccaccggaa gcgaaagaag ttcaaccgtg cggcacagtc gtttctcgat ctgttgcgaa
     3661 actcgccgga aaaggaaccg gaagccgtcc agcgcgccgc cactgcgaaa tcctgaaggc
     3721 ccgcttgtaa tcacatgcgc ccgcggcgaa ctgcgatgat ttcggtatcc tttgcggctc
     3781 cgccgatcgc ttgcagtttt atcaggactt caaatgcagg aaccggttct ccctcggcgg
     3841 ctccaatcgc cgccagaagc tgccccatta actgaggcgt gagcgcgaac tcgccgacgg
     3901 cctcggtgcc ttccatattc aagccttgca gaatcaggat ccggccactg cgcgacagat
     3961 tcggaacgag agagatcaaa ccatacgatt gtccggtttt ggcctcgctc gattccatga
     4021 catactctct ggattcgccg ggtcgtggcg atcggttggc gatatggatg cggtgagcgg
     4081 ctgaatcgta gactacggtg aagttgagtt tggactcaaa aagctccacc caggggtttg
     4141 cccgccggct tcccaagatg atggcattgc cgctgtgaaa atcgcgcact tgcagcgatc
     4201 tggcgaatcg aatgacggtg cgagtccggg gagacgttgc ggaaatgcgg gcggcgag
//
LOCUS       DASTMK010000021         8434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_65480, whole genome shotgun sequence.
ACCESSION   DASTMK010000021 DASTMK010000000
VERSION     DASTMK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65480"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFQ79_00760"
     CDS             complement(<1..242)
                     /locus_tag="VFQ79_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138203.1"
                     /translation="MSRYTVRPLDLDSLRTVSLRARGGKVRAADFARPYRKGEGFAGF
                     LDGLPHILAGDSVRAVIAAIQDAREHGKPVIWALGG"
     gene            complement(239..3001)
                     /gene="uvrA"
                     /locus_tag="VFQ79_00765"
     CDS             complement(239..3001)
                     /gene="uvrA"
                     /locus_tag="VFQ79_00765"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU0138204.1"
                     /translation="MLDRISVHGARQHNLKNIDVEIPRNTLTVITGLSGSGKSSLAFD
                     TIYAEGQRRYVETLSPYARQFLDQMERPEVDSIDGLSPAISIEQKTTSRSPRSTVGTI
                     TEIYDYLRVLYSSIGVPHCPQCGGIISQQTTEQILQQVTTLRQGDRVMILAPVVRGRK
                     GEYKKDLEKLLRQGFVRARVDGVLRSLEDEIVLDRRKNHTIEVVVDRLVVKPGIEPRL
                     EASIGTAAKLTGGLVLVAVVNGEEHLYSQKLACPDCGASVPQLEPRSFSFNSPYGACQ
                     TCTGLGSKWSFDPARLLADPGKSLFNGGLVSTACSSAITEKLRAWARRCRIDISKPFE
                     QLPRKTQTAVFEGSNGFSGILKLLEEIYEEASEGYREWLVGLMSPAMCPDCRGRRLRP
                     SSLAVKVKGLSIAELTEMPVAGTMSVVRGWELNARELQIAGRITEEIGNRLEFLCAVG
                     LGYLSLARSAATLSGGEAQRIRLATQIGSKLRGVLYVLDEPSIGLHPRDNNKLLDTLV
                     HLRDLGNTVLVVEHDAETIERADYVIDLGPGAGRLGGALVASGTPREIELNPASLTGR
                     YLSGQLEIPAPRVRRAGNGHRLVVHGAREHNLKNIEVEIPLGVLTVVTGVSGSGKSTL
                     VTDILYRALSKAIYRSREEPGAHESISGLEHVDKVIEIDQSPIGRTPRSNPATYTGVF
                     TPIRDLYAMLPESRERGYKPGRFSFNVKGGRCEACQGDGLKRIEMNFLPDVYVTCDIC
                     RGKRYNYETLQVKYKGYSIADLLEATVEEALGVLENIPVVRLKLQTLSDVGLGYIHLG
                     QSSTTLSGGEAQRIKLARELSKRQTGKTFYILDEPTTGLHFDDVKKLLEVLHRLVDLG
                     NTVVVIEHQMDIIKSADWIIDLGPEGGEAGGYVVAAGPPEQILRSRRSYTAQALAKVL
                     RNGNGA"
     gene            3163..5457
                     /locus_tag="VFQ79_00770"
     CDS             3163..5457
                     /locus_tag="VFQ79_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138205.1"
                     /translation="MSTEIRHTSTRRVDWRGPLLLLLLTTCAFWKLVLTKQYTFLESP
                     DPAHQILPWLQVQAAALRQGTIAIWDPYHFGGQSLIGQMQPGVTSPFTYLLLAFPLHS
                     GQVHTYFVHIWLALIHFAAALFAYWLFRDLGCRVAASVLGAMFYTLAGFSTIEWPQIL
                     AASIWAPLVFLFLLRSLDGRRPLANAGLAGLFLGLSWLSGHHQPPLFLSLAVVCIFVF
                     FLVRRPGFRRRLTTCAGVLFLITGLTAAVQVLPALEYGRLAMRWTASGALDWKAKVQY
                     PEHEQQGLRATDVLGMVIPGSSSSLASPFVGVVGISFAALGLALALRRRGAGLLLVVG
                     IGALLFSMPRYNVLHGLFYVLVPLVEKARSPAMAICVTQVCVAGLVAFGGDAFLAARN
                     SPWIRRVAVWLSGFGALTLGLTYLMLFLAPTVQSHLVTGDDRIVAVGLISLLLAGLFA
                     AYARGHLAAGTAVAFACLLLLIEQGTSIGFAWPHKNEVERTRFLKPMFESADIAQFLR
                     RQPEPGRVEVNRDDVAFNFGDWYGIETIEGYTPSMPAQFVELNWWNARVRQMYGVRYA
                     IAKNPMRPGQEEVFTSKSGLKVFRNADAFPPVWSVHRVETVPDLKQATELVRDGSLDL
                     RQTGVLVDRHAPALEECSQPDGIGRQWRALNWISVDVEMGCRGLVIAGANYAPGWRVQ
                     IDGKPARMMVVDTLLRGVVVPQGRHRIEMVYRPLSVYGGFTLTVAGLMLAYLLHRRRE
                     MNRESLLAPICIVKEARAATGILA"
     gene            5491..6507
                     /locus_tag="VFQ79_00775"
     CDS             5491..6507
                     /locus_tag="VFQ79_00775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0138206.1"
                     /translation="MAAKPTRRDFLAGSAGLVSTSALAAKALAQTSSASATGIPTRPL
                     GRTGVPVSIIGIGGWHIASLKDENESIRIMHTAIDEGVTFLDNCWDYHNGRAEEIMGK
                     ALVAGGRRKKVFLMTKNCERDYQGSLRNLEDSLRRLKTDYLDLWQFHEMVYDNDPDWV
                     FEKGGIKAAREAQQAGKLRFIGFTGHKDPSIHLKMLHKPHEWDAVQMPINLMDAHFRS
                     FQKEVVPVCLEKHAGVIGMKGLGGGDGRLVSKAGLTADECYHYCLSMPVSVQVMGITS
                     MAQLKQDIALARNFKPMDAAAKERLLRRVREEAGDGRHELFKTTKNFDGPHHRKQHGF
                     ETGD"
     gene            6504..6923
                     /locus_tag="VFQ79_00780"
     CDS             6504..6923
                     /locus_tag="VFQ79_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU0138207.1"
                     /translation="MKILLYYIGKPRDANANRIAEEYIKRTQRYAKCEMREIHPERAA
                     IWTRHSGAMKVLLDPGGKPMDSAAFAALLARHEMEGRDLLFVVGGADGFPADWKAQAD
                     LLLSLSKMTWPHELARAMLAEQIYRAVTTLRGHPYPR"
     gene            6935..7780
                     /gene="accD"
                     /locus_tag="VFQ79_00785"
     CDS             6935..7780
                     /gene="accD"
                     /locus_tag="VFQ79_00785"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU0138208.1"
                     /translation="MSWFTRKKPSVETGTPQERHVRTEGLWQKCDNCRQIVWRKTLEE
                     NLQVCPKCSFHFKIDARTRLKILFDGEWEELDTNLVSTDPLNFVDSKRYKDRLAAMQA
                     STSLPDALVSASGKLNGRPVNICALELKFIGGSMGAVMGEKITRAIEQSIEKRAPLII
                     VSASGGARMQEGAVSLMQLAKISAALMRLDEARVPYISVLTDPTTGGVTASFAMLGDL
                     NIAEPGALIGFAGPRVIEQTIRQKLPQGFQRSEFLLEHGFLDAVIQRSEMKEYIAKSL
                     NFFCN"
     gene            7835..>8434
                     /locus_tag="VFQ79_00790"
     CDS             7835..>8434
                     /locus_tag="VFQ79_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138209.1"
                     /translation="MKALVLCLLGLAVFPAAGYDLLIRNARVIDGSGNPWFHADVAVE
                     NGKIAAIGKLQDASAKRAVDARGLVLAPGFIDVHTHVEGGILKIPAAGNYVRDGVTTI
                     VTGNCGGSRVDLAGFFSDLEKTGIGLNVSSLIGHNSVRRDVMGTENRQATPEEIVRMQ
                     ELVEQGMRDGAVGFSTGLIYIPGTYANTEEVVSLAKAAAK"
BASE COUNT         1610 a         2566 c         2596 g         1662 t
ORIGIN      
        1 tgcccgccca gagcccagat cacgggcttt ccgtgttccc gcgcatcctg tatggccgct
       61 atgacggcgc gaaccgaatc gccggccagg atgtgcggca agccatcgag aaatccggca
      121 aacccttccc ctttccggta cgggcgcgcg aagtcggcgg ctcggacctt gcctccgcgt
      181 gcgcgaagag acaccgtccg caacgaatcc aggtccagtg ggcgaaccgt gtagcggctc
      241 acgcaccatt cccattgcga agaactttcg ccagcgcctg ggcggtgtag cttcttcgcg
      301 atcgcagaat ctgttccggt ggtccggcgg cgaccacata tcccccggcc tcaccgccct
      361 ccgggccgag atcgatgatc caatcggcgc tcttgatgat gtccatctga tgctcaatta
      421 ccacgacggt gttgccaagg tccaccagcc ggtgcaaaac ctcgagcagc ttttttacgt
      481 cgtcgaagtg cagacccgtc gtcggttcgt cgagtatata aaacgttttt ccagtttgcc
      541 gtttactgag ctcgcgggca agcttgatcc gctgcgcctc gccgcccgac agggtcgtgc
      601 tcgattgtcc aagatggatg tagcccagcc cgacgtcgct cagcgtttgc aacttcagcc
      661 tgacgacagg aatattctcc agaacgccca gggcttcctc gacggtcgct tccagcaggt
      721 ctgcgatcga gtacccctta tacttcacct ggagcgtctc gtagttatac cgcttgccgc
      781 ggcagatgtc gcaggtgaca taaacgtccg gcaggaagtt catctcaatg cgcttcaatc
      841 cgtctccctg gcacgcttcg cagcggccac ctttgacgtt gaagctgaac cgccccggtt
      901 tgtagccgcg ctcgcgcgat tccggcagca tcgcatagag atcgcgaatg ggcgtgaaca
      961 cgccggtata ggtggcggga ttcgaccggg gcgtccgtcc gataggggac tgatcgatct
     1021 cgatcacttt gtcgacgtgc tcgaggccgc tgatcgattc atgcgctccg ggctcctcgc
     1081 gggaccggta aatcgctttc gataacgcgc gataaagaat gtcggtgacc agggtcgact
     1141 tcccgctgcc cgaaacgccg gttaccaccg tcagaacacc gagcgggatt tcgacctcga
     1201 tgttcttgag gttgtgctcg cgagcgccgt gcaccaccag tctgtgcccg ttcccggccc
     1261 ggcgcacacg aggagcggga atctcgagtt gtcccgacag atagcgtcca gtgagcgaag
     1321 ccggattgag ctcaatttcc cgcggcgttc cgcttgcgac gagagcgcct cccaaacggc
     1381 ctgcacccgg ccccagatcg atcacatagt ccgcgcgctc gatcgtttcc gcatcgtgtt
     1441 ccacaacgag gacggtgttg cccaggtcgc gaagatgcac cagcgtgtcc agcagcttgt
     1501 tgttatcgcg cgggtgcaat ccgatcgacg gctcatcgag aacatagagc acgccgcgca
     1561 gtttggatcc gatctgcgta gcgagccgga tgcgctgcgc ttcgccgccg gagagagtgg
     1621 ccgcggaacg cgccaggctc aggtatccga gtcccaccgc acaaaggaat tcgagcctgt
     1681 ttccgatctc ctccgtaatg cgcccggcaa tctgaagctc gcgcgcattc aattcccagc
     1741 cgcgaacgac agacatggtt ccagccaccg gcatctccgt cagttccgcg atggaaagcc
     1801 ctttgacttt gacggcgaga ctgctcgggc gaaggcggcg tccgcggcaa tccgggcaca
     1861 tcgcgggtga catgagcccg accagccact cgcgataacc ttcgctcgcc tcctcataaa
     1921 tctcttcgag gagtttcagg atcccggaaa agccgttgct gccttcgaat accgccgtct
     1981 gcgttttccg gggcaactgc tcgaagggct ttgaaatatc gatgcggcag cggcgcgccc
     2041 acgcccgcag cttctctgtg atggcgctgg agcaggcggt ggaaaccagt cctccgttga
     2101 agagagactt gccggggtcc gcgagcagac gtgccggatc gaaggaccat ttgctgccca
     2161 gccccgtaca tgtttggcag gcgccatacg gactgttgaa ggagaacgaa cgcggttcga
     2221 gctgcggcac actcgctccg caatcggggc aggcgagttt ttgcgaatac aggtgctctt
     2281 caccgttgac caccgcgacc agcaccagtc cgccggtgag tttcgccgcc gtgccaatgg
     2341 aagcttcgag gcgcggctcg atgccgggct tcacaaccag ccggtctacc acgacttcga
     2401 tggtgtgatt cttccggcga tcgagcacga tctcgtcttc gagcgaacgg agaacaccgt
     2461 cgacgcgggc gcgcacaaag ccttggcgga ggagcttctc gagatccttc ttatactcac
     2521 cctttctgcc gcgcaccacc ggcgcgagaa tcatgacccg gtcgccctgc ctcagcgtgg
     2581 tgacttgctg gagaatctgc tccgtcgtct gctgcgagat gatgcctccg cactgcgggc
     2641 agtgcggcac tccgatcgag gaatacaaaa cgcgcaggta gtcgtaaatt tcggtgatgg
     2701 tgcccacggt ggaacgcggg ctgcggctgg tggttttctg ctcgatcgaa atcgccgggc
     2761 tcagcccgtc gatggaatcg acttccgggc gctccatctg gtccagaaac tggcgcgcgt
     2821 aaggggacaa ggtctcgaca tagcgccgct ggccctcggc atagatggta tcgaaggcga
     2881 gcgaggactt tcccgaaccg ctgagcccgg tgatgacggt cagcgtattc cggggaatct
     2941 cgacgtcgat gttcttcaga ttgtgttgcc gcgccccatg cacggaaatc cgatccagca
     3001 tagtgagagt cgggaggttg agccgaatat ctcattgtcc cacgatcccg cgccgccgca
     3061 gcgcgagggg gctggtacgc ggcgtgcgag atttcctgtg gattccacag agatgcctgt
     3121 aaacacggga ttccgggtct ttccactgta tcgatcggac gcatgagcac ggaaatccgt
     3181 cacacttcca cccgccgcgt ggattggcgc ggcccgctgc tacttcttct tctcaccacc
     3241 tgcgctttct ggaaactggt gctcacaaaa cagtacacat ttcttgaaag ccccgatccc
     3301 gcacaccaga ttttgccatg gctacaggtg caagcggcgg ctttgcgtca gggaaccatc
     3361 gctatctggg atccctatca cttcggcggc caatccctga tcggccagat gcagcccgga
     3421 gttaccagcc cgtttacgta tctgctgctg gcatttccgc tccatagcgg acaggtacac
     3481 acgtacttcg tccatatctg gctggcactg attcatttcg ccgcggcact gttcgcctac
     3541 tggctgttcc gcgatctcgg atgcagggtg gcggcgtcgg tccttggcgc gatgttttac
     3601 acgctggcgg gattctctac catcgaatgg ccgcaaattc tggccgcttc catttgggcg
     3661 ccgctggttt tcctgtttct cttgcgttcc ctggacggcc gccgtccgct tgccaatgcg
     3721 ggcctggcgg gtttattcct ggggctctcc tggctgagcg ggcatcacca gcctcctctt
     3781 tttctctccc ttgccgtcgt ttgcattttc gtgttttttc tcgtgcggcg gccgggattc
     3841 cgccgccggt taacgacgtg cgcgggtgtg ctgttcctga ttacaggact cacagcggcg
     3901 gtgcaggtgc tgccggcgct cgagtacgga cgcctggcga tgcggtggac cgcaagcggt
     3961 gcgctcgact ggaaagccaa ggtgcaatac ccggagcacg agcagcaggg gctgcgcgca
     4021 accgacgtgt tgggcatggt gataccgggc agcagttcca gtctcgccag tccattcgtc
     4081 ggcgtggtag gaatcagttt cgccgcgttg gggctggcac ttgcgctcag aaggcgcggc
     4141 gcagggttgc tgctggtggt aggcatcggt gcgcttctct tctccatgcc gcgctataac
     4201 gtactgcacg gcctgtttta cgtgctcgtg ccgctggtag aaaaagcgcg ctcgccggcc
     4261 atggcgattt gcgtgacaca ggtatgtgta gcgggcctgg tcgcattcgg cggagacgca
     4321 ttcctcgcgg cacggaactc gccctggatc cggcgcgtgg ctgtctggct atccgggttc
     4381 ggcgccctca cgctcgggct gacttatctg atgttgtttc ttgcgccaac cgtacagagc
     4441 cacctggtta ccggggatga ccgcatcgta gcagtcggac tcatcagcct cctgctggcg
     4501 ggactctttg cagcgtatgc ccgcggccat cttgcagccg gcaccgcggt ggccttcgcc
     4561 tgcctgctcc tgctgatcga acaaggcacg tccatcggtt tcgcctggcc gcacaagaac
     4621 gaggtggagc gaacgcgatt ccttaaaccg atgttcgagt cggccgacat cgcccaattc
     4681 ctgcgccggc aacccgaacc cggacgcgtc gaggtcaatc gcgacgatgt ggctttcaat
     4741 ttcggggatt ggtatggcat cgaaacgata gagggataca cacccagtat gccggcgcag
     4801 ttcgtggagt tgaactggtg gaacgcgcgt gtgcgccaaa tgtacggcgt gcggtatgcc
     4861 atcgccaaaa atccgatgcg gcccggacag gaagaggtat tcacaagcaa gagcgggctg
     4921 aaggtttttc gtaacgccga tgccttccca ccggtctgga gcgtacaccg cgtggagact
     4981 gtgccggacc tgaagcaggc aacggaactg gtgcgcgacg gcagtctcga tcttcggcaa
     5041 acgggcgtcc ttgtggaccg gcacgcgccc gcgctcgagg agtgctcgca gccggatggc
     5101 atcggccggc agtggcgggc cttgaactgg atcagcgtcg acgtggagat gggctgccgc
     5161 ggcctggtga ttgccggcgc caattatgcc cccggctggc gagtgcagat cgatggtaag
     5221 ccggccagga tgatggtggt cgacacgctc ctgcgtggtg tagtggtgcc gcagggccgg
     5281 catcggatcg agatggttta ccggccgctt tcggtttatg ggggtttcac cctcactgtg
     5341 gcagggctca tgctggcgta tctgctgcat cgccgccggg agatgaatcg ggaatccctt
     5401 ctcgccccga tatgtatcgt gaaagaggcg cgggcggcga cgggtatact cgcgtagcta
     5461 aaatgagagg aacaccaggg agggcctttc atggcagcaa agcccacgcg acgcgatttt
     5521 ttggccggca gcgccggctt ggtatccact tccgctctgg ccgccaaagc tctcgcgcaa
     5581 acaagcagcg cgtccgcgac gggcattccc acgcggccat tgggacggac cggggtgccc
     5641 gtttcgatca tcggaatcgg cggttggcac atcgcctcgc tcaaagacga aaacgagtcg
     5701 atacgcatca tgcataccgc gatcgacgaa ggcgtcacgt tcctcgataa ctgctgggat
     5761 tatcacaacg ggcgcgccga agagattatg ggcaaggcgc tcgtggccgg cggacgccgc
     5821 aaaaaagtct ttctcatgac gaagaactgc gaacgcgatt accagggttc gctcaggaat
     5881 ctggaagaca gcctgcgccg cctcaagacc gattacctgg acctctggca gttccacgag
     5941 atggtttacg ataacgatcc ggattgggtc ttcgaaaagg gaggcatcaa ggcggcgcgc
     6001 gaggcgcagc aggcagggaa gctccggttt atcggcttca caggccacaa agatcccagc
     6061 attcacctga agatgcttca taagccgcac gagtgggacg ccgtccagat gcccatcaac
     6121 ctgatggatg cgcatttccg cagctttcaa aaagaggtcg tgcctgtctg tctcgaaaag
     6181 cacgccggcg tgatcgggat gaagggttta ggcggcggag acgggcgcct ggtcagcaaa
     6241 gcagggctca ccgcggacga gtgttaccat tattgcctga gtatgccggt cagcgtccag
     6301 gtgatgggca tcacgtccat ggcgcaattg aagcaagaca tcgcgcttgc ccggaatttc
     6361 aagcccatgg atgcggcagc gaaggaacgc ctgctccgca gagtgcggga ggaagcgggt
     6421 gacggccggc acgagctgtt caaaaccacg aagaacttcg atggcccgca ccacagaaag
     6481 cagcatggct tcgagaccgg cgattgaaga ttctgctcta ctacatcgga aagccgcgcg
     6541 acgcgaatgc caaccgcatc gccgaggagt acatcaaacg cacgcagcgg tacgcgaaat
     6601 gcgagatgcg ggaaattcat cccgagcggg ccgccatctg gacgcgccat tccggcgcga
     6661 tgaaggttct gctcgatccc ggcggcaaac cgatggattc ggcggcgttc gccgctctgc
     6721 tggcccggca cgaaatggaa ggccgcgacc tgctgttcgt ggtgggcgga gcggacggtt
     6781 tccccgcgga ctggaaagca caggccgatc ttttgctctc tctttcaaag atgacctggc
     6841 cgcacgaact ggcgcgcgcc atgctggccg agcagatcta cagagccgtc accacattgc
     6901 ggggccatcc ttacccgcgc taatttagag cttcatgtcc tggttcactc gcaaaaagcc
     6961 gagcgtcgag acgggcacac cgcaggagcg gcacgtgcgc acggagggcc tctggcaaaa
     7021 gtgcgacaac tgccgccaga tcgtctggcg caaaacgctg gaggagaacc tacaggtctg
     7081 cccaaagtgc tcgtttcatt tcaagattga cgctcgcacg cgcctgaaga tcctcttcga
     7141 cggcgagtgg gaggaattgg acactaacct ggtttcgacc gatccgctca acttcgtcga
     7201 ttcgaagcgc tacaaggacc ggctggcagc aatgcaggcc agcacgtctc tgccggacgc
     7261 gctcgtgtcg gcctccggca agctgaacgg acgtccggtg aatatctgtg cgctggagct
     7321 gaaatttatc ggcggcagca tgggcgcagt catgggagag aagatcacgc gcgccattga
     7381 acagtcgatc gagaagcgcg cgccgctgat catcgtctcc gcatccggcg gtgcccgcat
     7441 gcaggaaggc gccgtcagcc tcatgcaact ggcgaagatc agcgcggcgc tcatgcggct
     7501 ggacgaggca agggtcccct atatcagcgt attgacggac cccaccactg gaggcgtcac
     7561 cgccagcttc gccatgctcg gagatctgaa tatcgccgag ccgggcgcgc tgatcgggtt
     7621 cgccggcccg cgcgtcatcg aacagaccat tcgccaaaag ctcccgcaag gatttcagcg
     7681 aagcgaattc ctgctcgaac atggattcct ggacgcggtg atccagcgca gcgagatgaa
     7741 ggagtatatc gccaaatcgc tgaacttctt ctgcaattga gacggcgcgt cgcggccttc
     7801 cccgcaaagg taaactgaga ggagatacct gttcatgaag gcgcttgtgc tctgcctgct
     7861 cggtctcgcc gtgtttccgg cagccggcta cgatctgctc atccgcaatg caagagtcat
     7921 cgatggctcg ggcaatccct ggttccacgc cgatgtcgcg gtggagaacg gtaaaatcgc
     7981 ggcaatcgga aagcttcagg atgcctccgc gaagcgggcc gtggacgctc gcggcctggt
     8041 tctcgctccg ggtttcatcg acgttcatac gcacgtcgag ggcggcattc tgaagattcc
     8101 cgcggccggc aactacgtgc gcgacggcgt cactacgatt gtgacgggta actgcggcgg
     8161 ctcgcgcgtg gacctcgccg gcttcttcag cgacctggaa aagacgggca tcggattgaa
     8221 cgtctcctcg ctgatcggcc acaactcggt gcggcgcgac gtgatgggaa cggagaatcg
     8281 ccaggccaca ccggaggaga tcgttcgcat gcaggaactg gtggaacagg gcatgcgcga
     8341 cggcgctgtc ggcttttcca ccggcctcat ctacattccg ggaacctacg ccaacaccga
     8401 ggaggttgtg agcctggcca aagccgccgc gaaa
//
LOCUS       DASTMK010000022         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_449399, whole genome shotgun sequence.
ACCESSION   DASTMK010000022 DASTMK010000000
VERSION     DASTMK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449399"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFQ79_00795"
     CDS             <1..245
                     /locus_tag="VFQ79_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU0138210.1"
                     /translation="QPREFAPPLDQFAAITENEFQTMFRGSPLKRARYTGFLRNVAIA
                     MGNSGHPGFHDPLVALTGSPDAVVAETAEWALGQLR"
     gene            594..>3032
                     /locus_tag="VFQ79_00800"
     CDS             594..>3032
                     /locus_tag="VFQ79_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEU0138211.1"
                     /translation="MRIKIKARKRKKSLKNIESTHVWARSPKYWMAVGTLAAYTAAGP
                     DPYAILYAQRPDVASPAAYSQESGALPARQFDIPAGPLDTVIAAFRIASGLQVDVAIP
                     AIGEVQSPGVSGFLPVEEALNKLLTGTGVTYHFTRPGRVVLELRGLRQSVEVTANVPL
                     SSPKYTEPMRNLPQTISVVPRAVIEQQGATSLSEVLRNVPGLTIAAGEGGVPAGDNLT
                     LRGFSARNDVFVDGVRDLGPQSRDPFNLEQVEVIKGPQSAFTGRGSTGGSINLVSKVP
                     NLNRFIGGSLSLGNASMQRFTADVNLPLARLGLGERTAFRMNFLEHDAGVPGRNVVES
                     ERRGFAPSLLFGGGTPTRLTLGYYRLHQNNIPDYGIPWVTPTQNVLADYRDQPAPVPR
                     DTFYGLKSRDREKMGSDMATVRFEHDFDDSLLLRNQLRYGRSTRDSITTAPRFANNDS
                     LTINRNGPSWLTEDDVWDNQTDLRANFQTGSVEHTAVLGATFSREHNRRKSRSVTGSP
                     TTTLFNPDPNQPFEGAITLNPIVGDATGDTQAFYAFDTVRFGQQFQLNGGLRWERFAV
                     DGLNTNGNPLDRTDTMLSGRAGAVYTPTQNGSFYVSYGTSLNPSLEGLTYQPADTTLE
                     PEKTYSVEGGTKWDLLNGRLSLSGAVFRVEKTNARTPGVLPDDPPMVLDGEQRVAGVE
                     IGVSGSITRRWSLYSAYTYLASKILTSNNPAEVGNELTNTPPHSLNIWTTYRLGKLEV
                     GGGTRFVDRRFNNNANVRRADGYWTADAMASYPIVSHLDLRLNLYNLNNAYYFERLGG
                     GHLIPGPARAGMITA"
BASE COUNT          622 a          900 c          919 g          591 t
ORIGIN      
        1 gccagccgcg cgaatttgcc cctccgctcg atcaattcgc ggcgattacg gaaaatgagt
       61 ttcaaactat gttccgcggc tcgccgctta aacgtgcccg atataccggc tttttacgca
      121 acgtcgcgat tgcgatggga aacagcggcc atccgggatt ccatgatcca ctcgtggcgc
      181 ttacgggttc tcccgacgcc gtggtggcgg aaacggccga gtgggccctg gggcagttgc
      241 gttaaagtct tttcacagac atgcgccttg gttgggggtt gcggcaggtt cgatgttccg
      301 ttcctgcatt tcggatcgcc gcaaggccgg tccaatgttt ttctaattga cactcaattg
      361 catcttggaa taatctgatg aagcaccgca tgtcttgcat gacggaactg ggccgcaagg
      421 caaattcagc gttttgaaat cgtaacgaag atccattaat gccctgacct ggcgcgtatc
      481 tctttggccg agcagcgcca ggccgggcgg atcggcaacc attgaggctg gcgacttgcc
      541 acgattgtaa gctgccagcc cctgtagttc aactgaaacg aggggacggc agaatgcgaa
      601 tcaagatcaa ggcacggaaa cgtaagaaga gcctgaagaa tatcgagtcc acccatgtgt
      661 gggctcgcag cccgaaatac tggatggccg tggggacgct ggccgcttac acggcggccg
      721 gacccgatcc gtacgccatc ctgtatgcgc agcggcccga tgtagcgtca ccggccgctt
      781 attcgcagga gtccggggct ctcccggcgc ggcagttcga cattccggcc ggcccgcttg
      841 acaccgttat tgccgctttt cggattgcat cgggattgca agtagacgtc gccattccgg
      901 cgatcggcga ggtgcaatct ccaggggttt ccggattcct gccggtggaa gaggcgctta
      961 acaaacttct tacgggtact ggagttactt accactttac gagaccgggc agggtggtgc
     1021 tggaacttcg cgggctgcga cagagtgtgg aagtaacggc taacgtgccg ctgtcgtccc
     1081 cgaaatacac cgaaccgatg cgcaatctgc cgcagacgat ctcggtggtt ccccgggcag
     1141 taatcgaaca gcagggcgca accagtctca gcgaagtgct gcgcaacgtg cccgggctga
     1201 ccatagcggc cggcgagggc ggggttccgg cgggagacaa tctgacactg cgcggtttca
     1261 gcgcccgcaa cgacgtgttt gtggacggcg ttcgcgatct cggtccgcaa tcgcgcgatc
     1321 ctttcaacct cgaacaggtg gaagtgatca aaggaccgca atccgccttc accggacgtg
     1381 gctccaccgg aggctcgatc aacctggtga gcaaagtgcc caatctcaac cgctttatcg
     1441 gtggatcgct gagcctcggc aacgccagca tgcagcgctt caccgcggat gtgaacctgc
     1501 cgttggcgcg cctgggattg ggagagcgaa ccgcttttcg catgaacttc ctggaacacg
     1561 acgcgggagt gcccgggcgg aacgtcgtcg agagcgagcg ccgcggtttt gctccttcgc
     1621 tgctgttcgg tgggggaaca cccactcgcc tgacgctggg ctattaccgt ttgcaccaga
     1681 acaacattcc ggactatgga attccgtggg tcacgcccac gcagaatgtt ctggctgatt
     1741 atcgggatca gccggcgccg gttccccgcg acactttcta cggactcaag tcgcgcgatc
     1801 gcgaaaagat gggctcggac atggcgaccg tgaggttcga gcatgatttc gacgactcgc
     1861 tgctgctgcg gaaccagttg cgttacgggc ggtcaacgcg cgactccatc accaccgcgc
     1921 cgcgctttgc caataacgat tcgctgacca tcaaccgcaa tggcccctcc tggctcaccg
     1981 aagacgacgt ttgggacaac cagaccgacc tccgggcaaa cttccagacg ggtagtgtcg
     2041 agcataccgc cgtgttgggc gccacgttca gccgcgagca taacaggaga aaatcgcgct
     2101 cggttacggg aagccccaca acgacgttgt tcaacccgga cccgaatcaa ccgttcgagg
     2161 gcgcgatcac gctcaacccg atcgttggag acgcaaccgg cgacacacag gctttctacg
     2221 cgtttgacac cgtccggttt ggccagcagt ttcagttgaa cggcggcctg cgctgggagc
     2281 ggttcgccgt ggatggtctc aatacgaacg gcaatccgct cgaccgcacc gacactatgc
     2341 ttagcgggcg ggcgggcgcc gtgtacacgc caacacagaa tggcagcttc tatgtctcgt
     2401 atggaacgtc gctgaacccg tcgctcgaag gtttgaccta ccagccggcg gacacgacgc
     2461 tcgaacccga gaagacctat agcgttgaag gcggaacgaa gtgggatctg ttgaacggac
     2521 gcctgtctct gagcggagcg gtgtttcgcg tggagaagac aaacgcgcga acgccgggtg
     2581 tgcttcccga cgatcctccg atggtgctgg atggcgagca gcgcgtagcc ggagtggaaa
     2641 tcggcgtttc cgggtctatc acgcggcgat ggtcgctgta tagcgcatac acctatctgg
     2701 cgagcaagat tctgacgtcc aacaatccgg ccgaagtagg aaacgagctg accaacacgc
     2761 ctccgcactc gctcaatatc tggacaacct accgccttgg aaagctggaa gtggggggcg
     2821 gtacacgctt cgtcgaccgg cgcttcaaca acaacgcgaa cgtgcggcgc gcggacggct
     2881 actggaccgc agatgcaatg gcttcgtatc cgatcgtaag ccacctcgac ctgcggttga
     2941 atctctacaa cctgaataac gcctactatt tcgagcgcct cggcggaggc catctcattc
     3001 cgggtcccgc gcgtgccggg atgattacgg cg
//
LOCUS       DASTMK010000023         1666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_97461, whole genome shotgun sequence.
ACCESSION   DASTMK010000023 DASTMK010000000
VERSION     DASTMK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97461"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFQ79_00805"
     CDS             <1..900
                     /locus_tag="VFQ79_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0138212.1"
                     /translation="PDFEREFGVDVRYGIYESNEEMLARVFSGNSGWDVVFPSNYLIL
                     PMRENNLLAPLQHGWLPNAGNLDRRFQSPAWDPSLDYSVPYMWGASGILFNRSLSPAP
                     ASWADLWEPRLDGRITMLDDPAEVLGACLKKIGESINATDPAVLLRAKEEAVQQKRLL
                     RAYLNAEVRDQVVAGDVLAAQLWATTAQQAMDAAPNLAFAYPSEGFAVYADTAVILRE
                     SERVRLAHQFIDYLLRPEVAAAVVRETRTATANRAARELLREVSPALYPADDVMARGE
                     WFEALPAPAQRLRDRLWTEIKAA"
     gene            complement(887..>1666)
                     /locus_tag="VFQ79_00810"
     CDS             complement(887..>1666)
                     /locus_tag="VFQ79_00810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0138213.1"
                     /translation="NARERLLAPSNFDTLAMGAIAGLAAALAATLLCMKFFERLHWVD
                     VGLWWRPATQWNLAVGLFAGLVSASVATVVPVLFGAGEFLPVASSDADLPQLIYFLVV
                     ITIAAAGEELQFRGYLFQVLVRATGIIPALLITGALFGMLHLGNPGSSHLGTLNTVLA
                     GVVLGYAFWRSGDLWLPIGAHIGWNCVFPLVGDELSGIGVHLTGYRIFWKSAALWHGG
                     SYGPEGGLYGTAGLLAMLVLVRRLPVKRQDAPLLESGATPL"
BASE COUNT          339 a          528 c          526 g          273 t
ORIGIN      
        1 ccggatttcg agcgcgagtt cggcgtcgat gtgcgctacg gcatctacga gagcaacgaa
       61 gagatgctgg caagggtctt ctccggcaac agcggctggg atgtcgtctt cccgagcaac
      121 tatctgattc tgcccatgcg cgaaaataac ctgctggcgc ctctccagca cggctggctg
      181 ccgaatgcgg gcaacctgga ccggcgtttc cagagcccgg cgtgggaccc atccttggac
      241 tacagcgttc cgtatatgtg gggcgctagc ggaattctgt ttaaccgttc tctatccccc
      301 gcgcccgcaa gttgggcgga cctctgggag ccgcggctcg acggacgcat cacgatgctg
      361 gacgatccgg cggaagtgct cggcgcctgc ctcaagaaaa taggggaatc gatcaacgcg
      421 acggacccgg cggtgctgct ccgcgcgaaa gaggaagcgg tgcagcaaaa acgattgttg
      481 cgtgcttacc tgaacgccga ggtgcgcgac caggtggttg cgggagacgt gctcgctgcg
      541 caactctggg ccaccaccgc gcaacaggct atggatgcgg ccccgaatct cgcattcgcc
      601 tacccgtccg agggctttgc cgtctacgcc gacaccgctg ttattctgcg cgagagcgag
      661 cgggtgcggc tggcgcacca gtttatcgac tatcttctgc ggccggaagt ggcagccgcc
      721 gtcgttcgcg agaccagaac agcgacggcg aaccgcgccg cgcgggaact gctgcgggag
      781 gtgagtccgg ccctgtatcc cgcagacgac gtgatggccc ggggcgaatg gttcgaagcc
      841 cttccggcgc ctgcgcagcg gttgcgcgac cgtctctgga ccgagatcaa agcggcgtag
      901 cgccggattc cagcagcgga gcgtcctggc gctttaccgg cagcctccgc accaggacca
      961 gcatggccag caatccggct gtcccgtaga gtccgccttc gggtccgtac gagcctccat
     1021 gccacaacgc ggcggatttc cagaagatcc gatacccggt caaatgcact ccgatgccgc
     1081 tcaactcatc gcccaccagg gggaagacgc aattccatcc gatatgcgca ccgatgggaa
     1141 gccacaggtc gccgcttcgc cagaacgcat atcccagaac gacgcccgcc agaacggtgt
     1201 tcaacgttcc caggtgtgag gacccggggt tgccaaggtg gagcatcccg aaaagcgcgc
     1261 cggtgatcag cagggcaggg atgatgccgg tggctctcac gagcacttga aacaggtaac
     1321 cacggaattg cagttcttcg cccgcggcgg caatggtgat aaccaccagg aagtagatga
     1381 gttggggcag atcggcatcc gaggaagcta ccggcaggaa ttcgcccgct ccgaacaaca
     1441 cgggaaccac cgtggcgacg ctggcggata ccagcccagc aaacaaaccc acagccagat
     1501 tccattgcgt tgccgggcgc caccagaggc ccacgtccac ccagtgcagc ctctcgaaaa
     1561 acttcataca gaggagggtg gcggccaatg cagcggcaag tccggcgatg gctcccatcg
     1621 cgagggtatc gaaattcgat ggggccagca gccgttcccg cgcatt
//
LOCUS       DASTMK010000024         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_577504, whole genome shotgun sequence.
ACCESSION   DASTMK010000024 DASTMK010000000
VERSION     DASTMK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577504"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFQ79_00815"
     CDS             <1..911
                     /locus_tag="VFQ79_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0138214.1"
                     /translation="SWITYGLGSECEDLPGFVVLQSGPRGPRGGALNWGSGFLPTRYQ
                     GVPFRSAGDPIIDLKNPEGVTRRSQRASIDLIRDLNRRRLDATGDDEISTRIASFEMA
                     YRMQTSAPELIDLSGETSETLALYGIERGAPSFAANCLLARRLVERGVRFIQLYHTDW
                     DHHADLGKPFEETCTEVDRPCAALVRDLEARGLLADTLVVWGGEFGRTPMGEVREAGK
                     AGRNHHIEATTMWMAGGGLKPGVSLGATDELGFSAVAHPIHMHDLQATILHLAGIDHT
                     RLTFKFQGRPFRLTDVGGKVLTEIIA"
     gene            complement(901..2037)
                     /locus_tag="VFQ79_00820"
     CDS             complement(901..2037)
                     /locus_tag="VFQ79_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEU0138215.1"
                     /translation="MKPKRPHRGLFARRALPLLLMFLPSLVSAATIYNQTNLVSDIPG
                     MATHTDANLVNPWGMSSSASSPMWVSNAGTSTSTLYNGQGTPQALVVNIPPPSGGSGF
                     SGPTGQVFNSGSGFELNPGQPARFLFATLSGTIAGWNPAANPTSAITKIDNSAFGAIY
                     TGLASAGDVLYAANFSAGAIDAFDAGFNPLTLQGSFSDPNLPAGYSPFNIQNVGGELY
                     VMYALFDPDEKEEVKGAGLGYVNVFDASGNLLRRFVSNGPLNAPWGVAMAPASFGDFG
                     GKVLVGNFGDGTIHAFEPSTGMLLGAIQGRGGSNIVNEGLWAIRFGNGGNGGDVNKLY
                     FAAGIDDEEHGLFGSIAPVPEPGTLTVCAGGLLVLLRLRRRVKR"
     gene            2164..>3938
                     /locus_tag="VFQ79_00825"
     CDS             2164..>3938
                     /locus_tag="VFQ79_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138216.1"
                     /translation="MRTRRLILISNCLLGLYFPVSAQSLKNAKALTIEGDLAAQMVSG
                     ISRFLDAELARAVERRPSLWKYDHSSAAAYQKSVEPNRGRLAAIIGLAGERVPKVELQ
                     FDAAYDRPSLVAKTPAYEIHTVRWPVYADMEAEGLLFEPAGTAKAAVIALPEADWSPE
                     QLAGLAPGFPAASQYARCLAEQGCRVLVPVLIDRKDTWSGNPRFRYTNQPHREFIYRM
                     AYQLGRHIIGYEAEKVRAAVDWIKAGTPALPIGVAGYGEGGLIALYSAAVDPRIDAAL
                     VSGYFESREGLWQEPIYRNVWSLLAEFGDAQLAALIAPRALVIEAVPGPSVSGPPAPS
                     QQRRGAAPGKLEGPDVAGVKREFARAAETYERLGVKDGIALVTPAAQSPGSTEALTRL
                     LASLRLTWVDAAASRPVDSRKDYDPNERLHRQFNQMVEHTQRVLRSAEVTRGKFWAKA
                     DLSSVDGWKRTSGWYRDYYWKEVIGKLPAPSEPVQPQVREVPGTDKWKRYDVVLPVWP
                     DVFAYGVLLLPKDLKPSERRPVVVCQHGLEGRPDHVILPEDKRTAQVYGNFAAKLADR
                     GFVIFAPQAPYIGDFRHLQRKANPL"
BASE COUNT          776 a         1184 c         1199 g          779 t
ORIGIN      
        1 gctcctggat cacttatgga ttaggaagcg agtgcgagga cctgcccggt ttcgtcgtcc
       61 tgcaatccgg tcctcgcggc ccgcgcggtg gagcgttgaa ttggggaagc ggatttctgc
      121 cgacgcgtta tcagggtgtg cctttccgct ctgctggaga cccgatcatc gatctgaaaa
      181 atccggaggg ggttacacgg cgaagccagc gcgcatctat cgacctgatc cgcgacctga
      241 accgtaggcg cttggatgcc accggcgacg atgagatttc gacgcgcatc gcgtcgtttg
      301 agatggccta ccggatgcaa accagcgctc cggagttgat cgatctttca ggcgagactt
      361 ccgaaacact tgccctgtac ggcatcgagc gcggtgcgcc ttcgttcgcg gccaactgtc
      421 tgctggcccg gcgtctggtg gagcgcggcg ttcgtttcat ccagctctat cacacggatt
      481 gggatcacca cgcggacctc ggcaaacctt tcgaagaaac gtgcacggag gtggaccggc
      541 cgtgcgcggc attggtccgc gatcttgaag cgcgcggcct gcttgcggac acgctcgtag
      601 tctggggcgg tgagtttggc agaacaccca tgggcgaggt tcgcgaagcg ggaaaggccg
      661 gccggaatca ccacatcgaa gcgactacaa tgtggatggc cggcggcggt ctgaaacccg
      721 gcgtatcgtt gggcgcaacg gacgagctgg gcttttcggc cgtcgcgcat ccgatccata
      781 tgcacgactt acaggcaacc atcctgcacc tggcgggcat cgatcatacc cgccttactt
      841 tcaaatttca ggggagaccc ttccggttga cggatgtcgg cggaaaagtg ctcacggaaa
      901 tcatcgctta acgcgccgcc gcaggcggag cagaactaac aaaccgccgg cgcaaaccgt
      961 gagagtcccc ggttccggaa cgggcgcgat agacccgaat agcccgtgtt cttcgtcgtc
     1021 aatcccggcg gcaaaataga gcttgtttac atcgcctcca ttgcctccgt tgccgaagcg
     1081 tatcgcccag aggccctcat tgacgatgtt gctgccgcct ctgccctgaa ttgctcccag
     1141 cagcatgccg gttgatggct caaaagcatg aatagttccg tcaccgaaat tccccaccag
     1201 cacttttcct ccgaaatcac cgaatgacgc cggcgccatc gcgacgcccc agggtgcgtt
     1261 gagtgggccg ttggagacga agcgtcgcag aaggttgccg ctggcatcga acacgttgac
     1321 atagcccaat cccgctcctt tgacttcttc tttctcgtcg ggatcgaaca gtgcatacat
     1381 cacatagagt tcgccgccga cgttctggat gttaaagggg gaataccccg cgggcaggtt
     1441 tggatcggag aacgaaccct gcagagtcag ggggttgaag ccggcatcga aagcatcgat
     1501 ggcgccggct gaaaagtttg cggcgtacag cacatcgccg gccgaagcaa gaccggtgta
     1561 gattgcgccg aaggctgagt tgtctatctt ggtgattgcg ctggtggggt tggcggcggg
     1621 attccagccc gcaatagtgc cgctcaacgt tgcaaacaga aatcgcgccg gctgtccggg
     1681 atttaattca aaacccgagc cgctgttaaa cacttgaccc gttggtcctg aaaagccgct
     1741 tccgcccgat ggtggcggga tattgacgac cagagcttga ggagtgccct ggccgttgta
     1801 cagcgtggaa gtggacgtcc cggcatttga cacccacatg gggctcgatg cgctggaaga
     1861 catgccccag ggattgacca ggtttgcgtc cgtgtgagtc gccatccccg gaatatccga
     1921 gacgagattc gtctggttgt aaatcgtggc cgcggagact aacgaaggta gaaacatcaa
     1981 caggagggga agagcccgcc gcgcaaacaa accgcgatgg ggtctttttg gtttcataat
     2041 ttcctttccc agaacttcca ccgccagtat actgttgagc gtggcgaagg gattccgaat
     2101 agtttgtaaa taactattcg taaataattt caatccgcga agaaggaaat ctcctgagaa
     2161 cagatgcgaa cgcgtcgttt aatcctcatc tcgaattgcc tgctgggctt gtactttccc
     2221 gtctccgcgc aatcgctcaa gaacgccaaa gctctgacga tcgagggcga cttggcggcc
     2281 cagatggtca gcggcatcag ccggttcctc gatgctgaac tcgcgcgcgc cgtcgaacgg
     2341 cgcccttcgc tttggaagta cgatcactcc tccgctgcgg cttatcagaa atctgtcgag
     2401 cccaatcgcg ggaggctggc ggcgatcatc ggtcttgccg gtgagcgcgt tccgaaagta
     2461 gaactacagt tcgatgccgc ctatgacaga ccgtccctgg tcgcgaagac accggcctac
     2521 gagattcaca ctgttcgctg gcccgtctac gccgatatgg aggcggaggg tctgctgttc
     2581 gagcctgccg ggaccgcgaa ggccgccgtc atcgcccttc ccgaggccga ctggtctccg
     2641 gaacaactcg ccggcctcgc gcccggtttt cctgccgcat cccagtacgc gcggtgcctg
     2701 gctgaacagg gctgccgcgt gctggtgccg gtgttgatcg accgcaaaga tacatggtcc
     2761 ggcaacccgc gattccgtta caccaaccag ccgcaccgcg agtttatcta ccgcatggcc
     2821 taccaactgg gccggcacat catcggttac gaagcggaga aggtgcgggc cgccgtggat
     2881 tggatcaagg ccggcacgcc ggcgctgccc atcggagtgg ccggttatgg agaaggcggc
     2941 ctcatcgcgc tctattccgc cgctgtggac ccgcgtatcg atgcggcgct tgtaagcggc
     3001 tacttcgagt cgcgcgaggg gctctggcag gaaccgatct accgcaacgt gtggtcgctc
     3061 ctggcggagt tcggcgacgc gcaactggcg gcgttaattg cgccgcgcgc tctcgtcatt
     3121 gaagcggtcc ccggaccgtc tgtttccgga cctccggctc cgagccaaca gcgccgcggt
     3181 gccgcgcctg gaaagctcga aggccctgat gtggccggtg tgaaacgcga gtttgccagg
     3241 gccgctgaaa cttacgaaag gctcggcgtc aaagacggca ttgcattggt cactcccgcc
     3301 gcgcagtcgc ccggctccac ggaggcgctc actcgccttc ttgcttcgct ccgcctgacg
     3361 tgggttgacg cagcggcttc tcgccctgtc gattcgcgca aggactacga cccgaacgaa
     3421 cggctccatc gccagttcaa ccagatggta gaacacacgc agcgcgtgct tcgctccgcc
     3481 gaagtaacac gcggcaagtt ctgggcgaaa gcagatctct cctccgttga cggctggaaa
     3541 cgcacctcgg gctggtatcg ggactactac tggaaagagg tcatcggtaa attgcccgcg
     3601 ccctccgagc ccgttcagcc gcaggtgcgg gaggtgccgg gcacagataa atggaagcgc
     3661 tatgacgtcg tgctgccggt ctggcccgat gtcttcgcat acggagtgtt gctgcttccg
     3721 aaggacctga agccttccga gcgaagaccc gtcgtggttt gccagcatgg cctggaaggc
     3781 aggcccgatc atgtgatcct gcccgaagac aagcggactg cgcaggtcta cggcaacttc
     3841 gccgcgaaac tcgcggaccg cgggtttgtc atatttgctc cgcaggcgcc atacatcgga
     3901 gacttccgcc acttgcagcg gaaagcgaat ccgctgaa
//
LOCUS       DASTMK010000025         5361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_257493, whole genome shotgun sequence.
ACCESSION   DASTMK010000025 DASTMK010000000
VERSION     DASTMK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257493"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VFQ79_00830"
     CDS             complement(<1..1265)
                     /locus_tag="VFQ79_00830"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0138217.1"
                     /translation="MTRRFFVSMAAAPAAAARPPAAQTPREPLIYRDYSRILPDYLRS
                     LAAQAYNLRNREIARLTTPEAIRTRQRWVTETFWRLAGGKPERTPLNARVTGSFDRQG
                     YRVEKIVYETQPGFHVSANLYIPTTASAPFPGVLFQMGHSSNGKAWDSYQRCCQGLVR
                     LGFLVLAFDPMGQGERTYYPGPNPSRSRLPGGADDEHTVPGKQMLLIGDSSVRLQTWD
                     AVRSLDYLASHPMVDPKRLASTGTSGGGTNTMLLSAVDDRLAAAAVACGNTENIACRN
                     FNPPGATDDAEQNFAGSGPLGFDRWDLLYPMAPKPLLFVPSANDFAGTYSPNYIASGL
                     EEFAKLQRVYAALGHADRIAWEDSPLPHNLAYGSRIAIYNWFLRWLKQEKPVNEEPEV
                     APEKDDVLFAAKGSTVLTLGSETPLTMLR"
     gene            1355..5065
                     /locus_tag="VFQ79_00835"
     CDS             1355..5065
                     /locus_tag="VFQ79_00835"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEU0138218.1"
                     /translation="MAQATDPKIDINSWLEDELYQQYLHDRKTVDDSWKHLFEENGSR
                     EPETGGNGSGTSSTTPQRQIAAELPETRALAAPQPAIEAGPAEQLALLRGPSGRIAEN
                     MEASLSIPVATSQRTIPVKVIDENRRIVNEHRTLLGNSKISYTHLTGWAVVQALKSYP
                     NVNNAFIRKQGEPFRWVRNQINIGIAVDVTGKDGNRSLMVPNIKDADGMDFQQFLAAF
                     DDIVRRARSSKLKVEDFQGTTISLTNPGTVGTVSSVPRLMPGQGAIIATGTIDYPAEY
                     AGVTDAMRATLGVGKVMTLTCTYDHRIIQGAESGSFLAKIEELLQGGDEFYEKIFADL
                     KMPYHPVRWEADRAATLPGKADPRISEIAKQAAVLQLINAYRVRGHLIADLDPLGSEP
                     SHHAELDPATYGLSIWDLDREFLTGTLGEAMGGGGPQPVATLRQILETLRRTYCGKIS
                     CEYMNIQVPEQKRWLQHRMEPTTNNWPLDIETRRWILERLIEAEEFEHFLHARYIGHK
                     RFSLEGGETAIAVLDHVLDNCADSGVHEVVIGMAHRGRITVLANVVGKDLKQIFSQFE
                     ENYDPTTTQGSGDVKYHLGATGLHRSRSHREIIVSVSPNPSHLEAVDPVVQGIVRPKQ
                     DRLGDTERKRVIPLLIHGDAAFAGQGVVAETLNLSQLDGYCTGGTIHLIINNQIGFTT
                     GPEEARSTAYPTDVARMVQAPIFHVNGDDPEAAVRAAQLAFDYRQEFGKDVVIDMICY
                     RRHGHNEADDPSYTQPILYRKIRQQPSVSVLYAERLAREGAITREEADQMRKRIAARF
                     SQAFDEARKAQERFEVSELSAVDTEQIGSYCPRTAVSRETLERVVNGLTTFPSDFRLH
                     PKLRGFIERRKEALNKDSVDWALGESLAWGSLVLEGTPARLSGQDSGRGTFSQRHLEY
                     YDSEDGHQYIPLKHLSPDQARFEVWDSSLSEYAVMGFEFGYSVADPLTLVMWEAQFGD
                     FANGAQIMIDQFIVAAEAKWGQPSGLVLLLPHGYEGQGPEHSSARIERFLTLGAEENI
                     QVVNCTTPAQYFHVLRRQMYGGADRRGVRKPLIIFTPKSLLRHAKAVSAVQEFTAGGF
                     REVLDDHSVPAYDRITRLLLCSGKIYYELLAAREEKKADHVAIIRIEQLYPFPQQLFR
                     DVLMRYPLSADVFWVQEEPRNMGPWRFMAESIQPMLETPKRNLRYVGRQEMASPAPGS
                     MRRHTQEQKEIIEDAFAPTPAARARSRTRRIR"
     gene            5123..>5361
                     /locus_tag="VFQ79_00840"
     CDS             5123..>5361
                     /locus_tag="VFQ79_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEU0138219.1"
                     /translation="MDTITLLGTATGLGLVAGFRLYATMLVLGLAVRFGWIDLAPQYS
                     QLSVLASDWMLALSGLAFVFEFVADKIPWLDTLWDA"
BASE COUNT         1073 a         1606 c         1633 g         1049 t
ORIGIN      
        1 ttgcgcagca tcgtcagcgg cgtctcgctg ccaagagtaa ggaccgtact gcccttggcg
       61 gcaaacaaca cgtcgtcctt ctccggcgcc acctcgggtt cttcgttcac tggtttctcc
      121 tgcttgagcc agcgcaaaaa ccagttgtaa atcgcaatgc gcgatccata agcgaggtta
      181 tgcggcagcg gggaatcttc ccaagcaatg cgatccgcgt ggcctaaggc ggcatagact
      241 cgttgaagtt tggcaaattc ctccaagcca ctcgcgatgt aattgggcga gtaagtgcct
      301 gcgaaatcgt tcgcgctcgg gacaaacaac aaaggtttgg gcgccatcgg gtaaagcagg
      361 tcccagcgat cgaagcccag cggtcccgag ccggcgaagt tctgttcggc gtcatcggtg
      421 gcgcctggag ggttgaagtt ccggcaggcg atgttctcag tgtttccgca ggcgacggcg
      481 gctgccgcca ggcgatcgtc aacggctgac agcagcatcg tattggtacc gccgccggat
      541 gttcccgtgg aagccagccg cttgggatcc accatcgggt gcgacgcaag atagtcgaga
      601 ctgcgcaccg catcccaggt ttgaagccgc acgctcgaat caccgatcag cagcatctgc
      661 ttgcccggta ccgtatgctc gtcgtcagca cctccgggaa gacggctgcg cgacgggttg
      721 ggtccggggt agtaggtgcg ctcgccctga cccatcggat cgaaagccag caccaaaaaa
      781 cccagcctta caagaccctg gcagcatctc tggtaggagt cccaggcttt gccgttggag
      841 gaatggccca tctggaacaa cacgccggga aaaggagcgc ttgcagtcgt gggaatgtag
      901 agattcgccg aaacatggaa ccccggctgt gtttcataca cgatcttctc cacccggtaa
      961 ccctggcggt caaacgatcc ggtgacgcgc gcatttaagg gcgtgcgttc cggtttgcct
     1021 cccgccagtc tccagaaggt ttccgtcacc caccgctggc gcgtgcggat cgcctccggc
     1081 gtcgtcagcc gcgcgatctc ccggttgcgc aagttgtacg cctgcgcggc aagcgagcgc
     1141 aagtagtcgg gcagaatccg ggaatagtcc cggtaaatca gcggttcgcg gggtgtttgc
     1201 gccgccgggg gccgcgcggc cgctgccggg gccgccgcca tggatacgaa gaatcgtcgt
     1261 gtcactctct atcgacgata caacgttatg tgctcgaaca acctggtgcg tcgggtatca
     1321 tggttacaag ggctgttttt cctctatacc tgcaatggcg caagctacag atcccaaaat
     1381 agacatcaac agttggctcg aagatgagct ctatcagcag tatttgcatg accggaagac
     1441 cgtcgacgac agttggaagc atctcttcga agagaacggt agccgtgagc ccgagacggg
     1501 gggcaatggt tcaggcactt cctccacgac tccgcagcgg cagatagcgg ccgaacttcc
     1561 ggaaacgcgg gcgctggccg cccctcagcc ggccatcgaa gccggcccgg ccgagcagtt
     1621 ggcgctcctg cgcgggccgt cgggacgcat tgcggagaac atggaggcga gcctctccat
     1681 tccggtcgcg acgtcgcagc gtaccatccc ggtcaaggtc atcgacgaga accgccgtat
     1741 cgtcaatgag cataggacgc tgctgggcaa tagcaagatc tcctacaccc acctcaccgg
     1801 ctgggcggtt gtgcaggctc tgaagtcgta tccgaacgtc aacaacgcct tcatccggaa
     1861 gcagggcgag ccgttccgct gggtgcgcaa tcagatcaac atcggcattg ccgtggatgt
     1921 gacgggaaaa gacggcaacc ggtctctcat ggtgccgaat atcaaagacg ccgatgggat
     1981 ggattttcag cagttcctcg cggcgttcga cgacattgtt cgccgagccc gctcgtccaa
     2041 gctcaaggta gaggattttc agggtacaac gatttcactg acgaaccccg gtactgttgg
     2101 cactgtcagc tctgtcccgc ggctcatgcc cggccagggt gccattatcg ctaccggcac
     2161 gatcgactat ccggccgagt acgccggagt caccgatgcc atgcgcgcca cgcttggtgt
     2221 cggcaaggtg atgaccctca cctgtaccta cgaccaccgg atcattcagg gggcggaatc
     2281 cggttcgttc ctggcgaaga tagaggagtt gttgcaagga ggcgacgagt tctacgaaaa
     2341 gatcttcgcc gatctgaaga tgccgtatca tcccgtccgg tgggaagcgg acagagcggc
     2401 tactctgccg ggcaaggccg accccaggat ttcggaaatt gcgaagcagg cggccgtcct
     2461 gcagctcatc aatgcttacc gtgtacgagg ccacttgatc gccgacctgg atcctctcgg
     2521 ctccgaacct agccatcacg ccgagctgga cccggccacc tatgggcttt cgatctggga
     2581 tctggaccgc gaattcctga ccggtacact tggcgaggcc atgggcggcg gtggtccgca
     2641 acccgtggcg acattgcggc agatcctcga aacgttgcgc cgcacctact gtggaaagat
     2701 cagttgcgag tacatgaaca tccaggtgcc ggagcaaaag cgctggcttc agcaccgcat
     2761 ggaacccacc accaacaact ggcctctgga tatcgagacc cgccgctgga ttctggaacg
     2821 cctgattgaa gccgaggaat tcgagcactt cctgcacgcc cgttacatcg ggcacaagcg
     2881 gttttcgctt gaaggcggtg agaccgccat tgcggtgctc gatcatgtgc tcgataactg
     2941 cgccgacagc ggcgttcatg aagtcgtgat cgggatggcc catcgcggcc gcattaccgt
     3001 gctggcaaat gtggtcggca aagatctgaa acagatcttc tcgcagtttg aagagaacta
     3061 cgaccccacc acaacgcaag gttccggaga cgtcaaatat catcttggcg ccaccggttt
     3121 gcaccgctcg cggtcccatc gcgaaattat cgtctccgtt tcgccgaacc cgagccatct
     3181 ggaggccgtc gatccggtgg tgcagggcat tgtccgtccg aagcaggacc gtctgggcga
     3241 taccgagcga aagcgcgtca ttcccctgct gatccatggc gacgccgcat ttgcgggaca
     3301 gggcgttgtg gccgagacgc tgaacctgtc gcagcttgac ggatactgca ccggcggcac
     3361 cattcatctg atcatcaaca atcagatcgg attcacgacc ggcccggaag aggctcgctc
     3421 cacggcgtat cccaccgatg tggcgcgcat ggtgcaggcc ccgattttcc acgtgaacgg
     3481 cgatgacccg gaggcggcgg tccgcgccgc tcagcttgcc ttcgactacc ggcaggaatt
     3541 cggcaaagac gttgtcatcg atatgatctg ctaccggcgg cacggccata atgaagccga
     3601 cgaccccagc tacacgcagc cgattcttta tcgcaagatc cgccagcagc cgtccgtctc
     3661 ggtcctctac gccgaacgcc tggcgcgcga aggagctatc acacgcgagg aagcggacca
     3721 gatgcgaaag cgcattgccg cgcgcttctc ccaagcgttc gatgaggcca gaaaagcgca
     3781 ggaacgtttt gaagtatccg aactcagcgc cgtcgacacc gaacagattg ggagctactg
     3841 cccgcgaaca gccgtttccc gcgagacgct ggagcgcgtc gtgaacggtc tcaccacatt
     3901 tccatccgac ttccgcctgc atccgaagct tcgcggtttc attgaacggc gtaaggaagc
     3961 gctgaacaag gattccgtcg actgggccct gggggaatcg ttggcttggg gctctcttgt
     4021 cctggaaggt acgcccgccc ggctgagcgg acaggatagc gggcgtggaa cgttcagcca
     4081 gcgccatctg gaatactacg acagcgaaga cggccatcag tatattcccc tgaagcatct
     4141 gtctcccgat caggcccgtt ttgaggtttg ggacagttcg cttagcgaat acgcggtgat
     4201 ggggttcgag ttcggctata gtgtcgcgga tccgttgacg ctggtcatgt gggaggcgca
     4261 gttcggtgat tttgccaacg gcgcccagat catgatcgat cagttcatcg tcgcggcgga
     4321 agccaagtgg ggccagccga gcgggctggt tcttctgctg ccgcacggat atgagggcca
     4381 ggggccggaa cattcgagcg cccgcattga gcggtttctg acgcttggag cggaggagaa
     4441 cattcaggta gtgaattgca ctactccggc gcagtacttc catgttctgc ggcggcagat
     4501 gtacggcggt gcggaccggc gcggtgtccg gaaaccgctg atcatcttta ctcccaaaag
     4561 cctgttgcgg cacgcaaagg cggtctcggc cgttcaggaa ttcaccgccg gcggattccg
     4621 cgaggtgctg gacgaccact ccgttcccgc ttatgatcgc attacccgcc ttctgctttg
     4681 ctccgggaag atttactatg agttgctggc ggcaagggaa gagaaaaagg cggatcacgt
     4741 cgcgatcatt cgtatcgagc agctttatcc gttcccgcaa cagttgtttc gcgatgtgct
     4801 aatgcgatat ccgctcagcg ccgacgtctt ctgggtgcag gaggagccgc gcaacatggg
     4861 accctggcgg ttcatggccg aaagtatcca gcctatgctg gagactccca aacggaatct
     4921 gcggtacgta gggcgccagg agatggcgag tcctgctccg gggtcgatgc gccgccacac
     4981 ccaggaacaa aaggagatca tcgaagacgc tttcgctccc acgccggcag cccgcgcccg
     5041 ctcccggacg cgccggattc gttgacggcg caggcttgat ttcgagaaca aagcgagcgg
     5101 cttttgcgtc ctgaggaggt agatggacac cattactctg ctcggcactg ccacggggct
     5161 gggtcttgtg gccggcttcc ggctttacgc cacgatgctc gtcctcggcc tggcggtgcg
     5221 gttcggctgg attgacctgg cgccgcagta ttcccaattg tccgtcctgg ccagtgactg
     5281 gatgctggct ctgagcgggc tcgccttcgt tttcgagttc gtcgccgaca aaatcccctg
     5341 gctggatacg ctgtgggatg c
//
LOCUS       DASTMK010000026        12365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_257700, whole genome shotgun sequence.
ACCESSION   DASTMK010000026 DASTMK010000000
VERSION     DASTMK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257700"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..468
                     /locus_tag="VFQ79_00845"
     CDS             226..468
                     /locus_tag="VFQ79_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138220.1"
                     /translation="MNNDSNRRIKGVVAVFEHRVSEPFFTRRRIKPNGHLEEIIVGAP
                     FATVVIALSMMAMVIVLTLLDVDMNVLLPVIAKLGS"
     gene            620..1195
                     /locus_tag="VFQ79_00850"
     CDS             620..1195
                     /locus_tag="VFQ79_00850"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEU0138221.1"
                     /translation="MMPKKDNMGEELPVPAELIEQRIFLIRGHKVMVDADLASLYDVP
                     TGALNRAVKRNLERFPEDFCFQLMNEELEALRFQSGISKKVGRGGRRYLPYVFTEQGV
                     AMLASVLNSPRAVQVSIGIVRVFVRLRAILATHAELSRRLEELERKFEKHDSELYYVF
                     EAIRELMAPEPVPPKRRIGFQAGPDDSGAIS"
     gene            1380..1736
                     /locus_tag="VFQ79_00855"
     CDS             1380..1736
                     /locus_tag="VFQ79_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138222.1"
                     /translation="MIRYVRFLRDKAARGPVSKGFLDGWYTFHEVKQNWLGFSKCGNN
                     GSGLGPTFARAMHANLNSVFKELGLLGHEDIILIDARKDNKPSASVGTRGTSDLHPCA
                     GTISLCALMVSHLRFS"
     gene            1825..2250
                     /locus_tag="VFQ79_00860"
     CDS             1825..2250
                     /locus_tag="VFQ79_00860"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEU0138223.1"
                     /translation="MSRPSVHRKRGRAARPWVPPATRIHLRPEADDLSDVRPLLARIT
                     GALGTNAAARLLGADRAQVSRWGADKESVGAEMSRRIVDLHDVLTRILRIYHRDAAAM
                     WLSGPEPLLGGARPIDVLALEGAAPVVRAIEAIAEGAYA"
     gene            2256..2837
                     /locus_tag="VFQ79_00865"
     CDS             2256..2837
                     /locus_tag="VFQ79_00865"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU0138224.1"
                     /translation="MATLYRVFPCQPEAAPVEPGGALYIPPQGAGRIDNPGLYAVLYL
                     SDTAEGAIAEAFGRFPEWTPAILAGSPALPGSTRAIAQYRFRGELPLCDLDDAARLLA
                     LELRPSQVVTRDYTQTRAWARTIFEQGRWAGIRWWSYYRPEWGSIGLWSVAALTLETI
                     RPLRLDDVDLIAASRTIARRVTAAAARKSGASR"
     gene            2963..4711
                     /locus_tag="VFQ79_00870"
     CDS             2963..4711
                     /locus_tag="VFQ79_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEU0138225.1"
                     /translation="MAANTYPLIRSRFYPAAPAVQNHESQAENLHRAGRALWAAGLYE
                     RALAPIGRAIALDPHVPAYHHSLGQTLHRLARFSDAALCYQEALRLEPASAGVLNDLG
                     AALIECRRFPEAVSALLDAIAIQPGFPEAFNNLGSALREQGQYDEAIACYGEALRLRP
                     GYAQAHNNLGTAFEKSGRLEEALAEYGWAVRLDPGYAQARFNRAAARLLKGDFERGWK
                     EFEWRFQAAGHAPRQSTAPLWDGSPLQGRRILLHTEQGLGDTLQFVRYAGLVKQRGGV
                     VILQCQPRLVELLRTVQGIDEIAAGDIPAHDCHAPLLSLPLLFHTLPETVPAPVPYIR
                     PDPTLAAKWAERMGRKHSLRVGLVWAGNPNQLNDRYRSMSLAQLAPLASIEGVQLFSL
                     QRGEKAQELLENQAGWRIARIEREDEGIAGTAAMIETLDLVITVDTMVAHLAGALAKP
                     VWTMLPFAPDWRWLLDRNDSPWYPTMRLFRQPRPGDWESVVREVSEALRGEAFSRQPP
                     AVSQPGLADSRALIAEALLSFAAGLSPVPSQSDSREREPLIRMRLRSPLRQSPAPAAL
                     LPPAPFPATEYAPPGV"
     gene            complement(5637..6782)
                     /locus_tag="VFQ79_00875"
     CDS             complement(5637..6782)
                     /locus_tag="VFQ79_00875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0138226.1"
                     /translation="MTGRNGRDTLYLALPVGSTHGWGICGRYIAREMARLTTVHLITD
                     PFQTVGNEMEDRFLQTLLPSASEFTGTAPLLQGITGKDLMPCRPDLRGQPQIGYTFFE
                     ENILPAESIGNARTQFDVVVTGSSWCKDVLEAHGLENVSTIVQGIDPQIFHPTPYDKD
                     LFSDRFVVFSGGKFEFRKGQDLAIRAYKVLQDRHPDVMLVHSWYNPWSFSLDTMRSSP
                     YIRFAPSSTDYFAAMNQVLNDNGIDVSRVIALPPRPNALMAQFHRQTDVGLFPNRCEG
                     GTNLVLMEYMACGKPVVASWSTGHKDILTGENAILIRNMGRLHIRNGESLVAVWDDPN
                     LEETIEQLEWAYRNREKLKAIGRQAGEDLQRFTWTETARQFLGLLRN"
     gene            complement(6882..8360)
                     /locus_tag="VFQ79_00880"
     CDS             complement(6882..8360)
                     /locus_tag="VFQ79_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEU0138227.1"
                     /translation="MAISINTNVNALIAQENLRVNSDFQGRTIQRLTSGYRINSSGDD
                     AAGLAVANKFRSDTAELMQGVRNANDGLSQLQIIDGGLNNISKMLDRLKTLATQSASV
                     TFTGKRETLNNEYQALLNEIDRQAANIGLDSNGTFNKVIDVYTGGGGETQANSKVSVD
                     LSGDSNVVTYDKLGLSGTNIAAGGVSLAGNNVRLDNVAGTFSGDATLTFHMYRNNQNS
                     TVAITYTHDAASGQEAVAKLNEQLSDYGIAASIGGDGKLRLNSGVAFSVSVAAAANSI
                     ATDAVSADANTSLNSVMAEADFVGMSATESETALVTTQKGTYTLALNDTNAATIGAAV
                     SYINQQLGGSGVYAITNAAGNRISLQSAEEFSASVVANVGAGVAFKAGTTTGAVAYTA
                     ASSGDGSAAEAAIKKITAAVEQLGTVQGRVGTGQNKLQYAITLAQSQISGFSAAESRI
                     RDADVAEEAANLTKAQVMQQASIAAMAQANAAPQAVLSLLRG"
     gene            complement(8381..10507)
                     /locus_tag="VFQ79_00885"
     CDS             complement(8381..10507)
                     /locus_tag="VFQ79_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0138228.1"
                     /translation="MTTILDQLTTALEQQKRGRRLEAEALYRGILNENPENADAWHLL
                     GMLFYEAGQHVAAASHIAKAIRLEPGIPMFHNNLGNVMLAIGRPEKAVYCYEQAIRCR
                     PDFADSYTNLGNALQELDRPADSIFWYRKSIELKPGCAATYTCLGNALRSLGDAGGAE
                     ASFRKALELNPDGAEAHMNLGNLYYDRCDHQTAERHYRKAIESKRSLVQALSNLGAVL
                     RVQSKLDEAEQFCRQALSLQPNYPEAHSNLGAILLEKGELEQAEFHCRRAVRLKAALP
                     QAQVNLGLVLGRRHRFEEAESCYREALRLKPGYGAAYSALGRALCEQGRFKEASKALE
                     EALEATPGFVEAYCNLGYAALCRHDYKGALQWLNRAVELAPDDVSARFGRAIVLLLTG
                     DFKRGWREYEWRWKRNDVPGRTFPQPEWDGRVLNGETVLVYAEQGLGDSIQFVRYLDA
                     VRERGAKIVLECQPGLVALFAESRIAADQMIAKGADIPHFDFHVALLSLPRVLETGAG
                     APAGAAPYLSVDSARLAQWHEFIAGYSGLKVGLVWTGNVQNTNNRHRSVPLESLAPLD
                     LPGVTLFNLQMGVEASEVQRGGLPMVDLSGRQPDIRDTAAAMRNLDLVITADTMTAHL
                     AGALGVPVWTLLCYNPDWRWGIEGNRTPWYPSMRLFRQPAWGDWASVITEVVKQLQTL
                     VQGSNGCTDEFSDGKGWPRLAGSQQH"
     gene            complement(10583..10894)
                     /locus_tag="VFQ79_00890"
     CDS             complement(10583..10894)
                     /locus_tag="VFQ79_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138229.1"
                     /translation="MAKKPAATAARLRKAVAEGRYADARDLAATYQTELEAAARALAP
                     DSPEAHTLEICSRQLLEWALKAARAARAHHSAQWKELNHSHSPYPAAAVPAGSSWEFD
                     A"
     gene            complement(10894..>12365)
                     /gene="fliD"
                     /locus_tag="VFQ79_00895"
     CDS             complement(10894..>12365)
                     /gene="fliD"
                     /locus_tag="VFQ79_00895"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEU0138230.1"
                     /translation="SRDRQEAVAFIKAQRLLCRYVMGRRMSSTIFTGSSRYASDFQSV
                     IDRAVAIASLPLSQMQSERGVLNSQSSAASSLKTKVDQVQSAISTLSASLGLNSYKSS
                     VSDTSIVKANLAEGVLAGTYTVQVKELGAYTNTMGKNWADADPVTLQVKDASKENLTA
                     SSTYQLKILDGEGAVRTSYDIDLAGKNNLNALVEQINKQAGGDVQASVVNVGGSTPDY
                     RISIQLKTLSTDQIQLTDGTNDLLDQLTAGAPAQYIVNGSSTVATSDSRTVNIAPGLT
                     VDLLKQQESTADPVTITVSRDTNSVSNALSSFVNAYNAAVTELDVHRGQGNGVLKGHS
                     IIATVADTLRSTASYSSGTGEISSLADLGLEFDDKGKLSLNSATFSANTKDQFEKMLA
                     FFGSTAEGGFLQSAATAVNGLQSSDNSVLGLTISSLSNTLKSQDARIAAEQERIGNLE
                     TRLQAQMAEADALIAMLEQQVTYFTNMFESMRVASRGMS"
BASE COUNT         2260 a         3782 c         3651 g         2672 t
ORIGIN      
        1 gtaaaccgag ccttcggtgt ggcatctgtc ttcagtgcgg ggactgatct caacaagttc
       61 tgtccggctg agtaggctta tttcgaatga ttccagtgac ttgcaggagt gctgtcgcta
      121 ttctctcaaa cttgccgcac actatactcg cgggccgctc gaaaaagtag ttcgcaacag
      181 agcacttgac cgaggggcca tcgatctccg actcatatgg cggctatgaa caacgacagc
      241 aatcgacgta ttaaaggggt ggttgcggta ttcgagcacc gggtctccga accattcttt
      301 acccgcaggc gcatcaagcc caacgggcat cttgaagaga taattgtcgg ggcaccgttc
      361 gcaactgttg tgatcgcgct ctcgatgatg gcgatggtca tcgtactgac gcttctcgac
      421 gtcgacatga acgtattgct acccgtgatc gcgaagcttg gttcatgatg cgggctcggg
      481 agcaaacgcc gccgattcga gcggcaacta ccgcaccggc accaggggat gttcgtacac
      541 catacgaccg tttcgagata tccgcgcaac ggcgcattga cgaacggggt tggagtgata
      601 cgctttgggg tgtaatctca tgatgccgaa gaaagacaac atgggcgagg aactgcccgt
      661 tcccgcggaa cttatcgaac agcgcatatt cctcatccga gggcacaagg tgatggtcga
      721 cgccgatctc gcctctctct acgacgttcc gacgggtgcc ctcaaccggg cggtcaagcg
      781 caatctcgag cggttcccgg aggacttctg cttccaactc atgaacgagg agctcgaggc
      841 tttgagattc caatctggca tctcaaagaa ggtgggccgc ggcgggcgcc ggtacctacc
      901 gtacgtcttc accgagcagg gggtcgcgat gctcgcgagt gtcctcaaca gtccccgcgc
      961 cgtccaggtc tcgatcggta tcgtccgcgt gttcgtccgg ctccgggcca tcctcgctac
     1021 gcatgcggag ctcagccgca ggctcgagga actcgagcgg aagttcgaga agcacgacag
     1081 cgagctttat tacgtcttcg aggcgatccg cgagctcatg gccccagaac ccgtgccgcc
     1141 gaagcggcgc atcggcttcc aggccggccc ggacgactcg ggggcaatct catgaccgcg
     1201 aatgcgccga acggcgccgt catcgacatc tacttcactg acttcttcga agttgacccg
     1261 tcgatctgga agcgtacggc gcattcaaca tttccctcat aaacgacctg ccgctcttca
     1321 tcgatccgtt tcttctcttc aacagcgaca agtccgaata ccagaagctc cacgacggca
     1381 tgatccggta tgtccggttc ctccgcgaca aggccgccag aggcccggtc tcgaagggct
     1441 tccttgacgg gtggtatacc ttccacgagg tgaagcagaa ctggctcggc ttttcgaagt
     1501 gcggcaacaa cggcagcggg ctcggcccga ccttcgcccg cgcgatgcac gcgaacctca
     1561 atagtgtgtt caaggagctt gggttactcg gccacgaaga catcatcctc atcgacgcga
     1621 ggaaggataa caagccgtca gcctcggtgg gcacgagagg aacttcggat cttcacccgt
     1681 gtgctggaac catctcgctc tgtgcgctca tggtatccca tcttaggttt tcgtgagtgt
     1741 gaaaagctcg aacgtctctt ctttcagccc ccagtgttgt gccctgatct caacaggttc
     1801 tgttgagtta taatcagaag tggaatgagc cggccatccg tccatcgaaa gcgcgggcgc
     1861 gcggcgcgcc cttgggtccc gcccgctacc cggatccatc tgcgtccgga ggcggacgat
     1921 ctgtccgacg tgcgcccctt gctggcgcga atcaccggcg ccctgggcac gaacgctgcc
     1981 gcccggcttc tcggcgcgga ccgggcgcag gtctcgcgat ggggtgcgga taaagaatcc
     2041 gtaggcgcgg agatgtcgcg ccgtatcgtc gatctgcacg atgtgttgac caggattttg
     2101 cgcatttatc accgggatgc ggcggccatg tggctctccg ggccggagcc gttgctcggc
     2161 ggcgcgcgtc cgatcgatgt cctggcgctt gaaggagccg ctcccgtggt tcgcgccatt
     2221 gaagcgattg cggaaggcgc atacgcctga gcgcggtggc aaccctctat cgcgtctttc
     2281 cctgccagcc ggaggccgct ccggttgagc ccggtggcgc actctatatt cctcctcaag
     2341 gcgccggacg gattgataac ccgggtctgt atgcggtgtt gtacctgagc gacactgccg
     2401 agggggccat tgccgaagct ttcggccggt ttcccgagtg gacgccggcc atcctggcag
     2461 gcagtccggc gctgcccgga agcactcgcg ccatcgcgca gtatcgattc cgtggagagc
     2521 ttccgctttg cgacctggac gatgccgcgc gcctcctcgc tctcgaactc cggccctcgc
     2581 aggttgtcac ccgggattac acgcaaaccc gcgcctgggc gagaaccatc ttcgagcagg
     2641 gccggtgggc cggcattcgc tggtggtcct actaccggcc cgaatggggt agcatcgggc
     2701 tctggagcgt ggcggcttta acgctggaaa ctatccggcc cttgcgcctg gacgatgtgg
     2761 acctgattgc cgccagccgg acgattgcgc ggcgtgtaac ggccgccgct gcgcgcaaat
     2821 ccggcgcttc ccggtagcct gccacggcgc ccgattggcc aaagcttcgt cacgcggcaa
     2881 aatcccgcca tccgcattac gtgtaaatta ctgaaaacgc gacaaccccg ttggatggat
     2941 aggtgcaacc attctctgga agatggctgc gaacacatac ccgttgatcc ggtcccggtt
     3001 ttatccggcc gcgccggctg ttcagaacca cgagtcgcag gcagagaatc tgcaccgcgc
     3061 aggcagagca ttgtgggccg cgggtttata tgagcgggcg cttgctccga tcggaagagc
     3121 cattgctctc gacccgcacg tacccgccta ccaccacagc ctggggcaaa cgcttcatcg
     3181 cctggcacgg ttctccgatg ccgccctatg ctaccaggaa gccctgcgcc ttgaaccggc
     3241 ttcggccggc gtgctgaacg atttaggcgc ggcattgatt gaatgccgcc gctttccgga
     3301 agcggtctcc gcgctgttgg acgctatcgc catccagccc ggttttcccg aggccttcaa
     3361 caatctcggc agcgcactgc gggaacaggg ccagtatgac gaggcgatcg cgtgctatgg
     3421 cgaagcactg cggctgaggc ccggatacgc gcaggcgcac aacaatctcg gcacggcgtt
     3481 cgaaaaaagc ggccgcctgg aagaggctct tgccgaatac ggctgggcgg ttcgcctcga
     3541 tccgggttat gctcaggccc gcttcaaccg cgccgcggcc cggcttctca aaggcgactt
     3601 cgagcgtgga tggaaagaat tcgaatggcg ctttcaggcc gccgggcatg ctccgcggca
     3661 atctacagcg cccctgtggg acggatcacc gctccaaggc aggcgcattc ttctccatac
     3721 cgaacaaggg ttgggcgaca cccttcagtt tgtccgttat gcgggcttgg tgaaacaacg
     3781 cggcggggtg gttattctcc aatgccagcc gcggctggtg gagctcctac gcaccgtgca
     3841 ggggattgat gaaatcgccg ccggcgacat tcccgctcat gactgccacg cacctctcct
     3901 gagcttgccg ctgctgtttc atacgcttcc tgaaaccgtg cccgcgccgg ttccctacat
     3961 ccggcccgat cccacactgg cggcaaagtg ggccgagcgc atgggccgga aacattcgct
     4021 gcgggtgggc ctggtttggg caggcaaccc gaaccagctc aacgaccgct atcgctccat
     4081 gtccctggcg caactcgctc cgcttgcttc tatcgaaggc gttcagctct tcagcctgca
     4141 aagaggggag aaagcgcagg agcttttaga gaaccaagcc ggatggcgta tcgcgcggat
     4201 tgagcgggaa gacgagggga ttgccggcac ggccgccatg attgaaaccc ttgacctggt
     4261 catcaccgtc gataccatgg ttgcgcacct ggcgggcgct ctcgcgaagc cggtgtggac
     4321 gatgctgccg tttgctcccg attggcgctg gctcctcgac cgcaacgact cgccgtggta
     4381 tcccactatg cggctgttcc gccagccccg gccgggggat tgggaatccg tagtccgcga
     4441 ggtgtcggaa gcgctgcgcg gcgaagcttt cagccgccag ccaccagcgg tcagccaacc
     4501 cggtttagct gacagccgag cgctgatcgc tgaagcctta cttagctttg cggccggcct
     4561 ttcgcctgtt ccatcgcaat ctgattcacg tgagcgagag cccttgatcc ggatgcgcct
     4621 tcgatcgcct ttaaggcaat ctcctgcacc tgccgctttg cttcctccag cgccttttcc
     4681 agcgacagaa tatgctccgc ctggcgtttg agagtttctt cgagcgtttt cacctgtagc
     4741 tcggcgactt tgcgctcggc ctgggcgtct ttttgcaata ccagaatctg ctgctcgtag
     4801 ccggcggacg cctggcgtgt ggcttcggcc agagccgatt ccctttccgc ggcgaggcgc
     4861 ttcggtatct cctcgacttc gcggcgcagc cgcgcgagct cctcttcttt ttctttcagc
     4921 gctgcttcgc gctgggccca gtcgcgttcc aactcctcct gccgctcttc gttctgcttt
     4981 tcccgcagcc gcatttcctc gtcgtactta tcctgcgcct tctttcgctc gagctgcttt
     5041 cgatattcgt aatcgtcgtt ctcgcgctgg cgctgttttt tcaaagcttc ttccgcttct
     5101 ttgcgttcgc gctccgccgc tcgcgcttcc tgctcccatt cgctgcggcg ggtctggatt
     5161 tcttcctcca gccgttcccg ttcgcgctca taatcctgaa ccatctgatc cagcgacgtg
     5221 gcggcgatat cgatcttgtg gaggcgttcc agctcctgcc gctccagttg aaccgcctcg
     5281 cgcaccttcg ccagcagatc gacttcgcct gtcagcctct cggtcatatc gatcaacgtg
     5341 cgcgagatct ctgtgcctaa cgtagagatc tgccgggtca cggaatcgac cgatatttcc
     5401 tccaccgctt gccggacctc cgccgaatgg cgttgttcgg cctctaccat cttcggattg
     5461 gcggattccc gggttgcttc cgtctcccgg cggaccgcct cgaattcctg ctgcacttca
     5521 gccttcgtcc gccgggtccg gctgggcgcg accgtcttcg ttgctttggt agccatcttt
     5581 ttttcgctta aggatctatc taagcactgt cgtgcggcgg aaaccaagcg ggccgctcag
     5641 ttgcgaagaa gccccagaaa ttgacgcgcg gtctcagtcc aggtgaagcg ctgcaaatcc
     5701 tcaccggcct gccggccaat ggctttcagc ttttcgcgat tccggtaagc ccattcgagt
     5761 tgctcgatgg tctcttccag gttcgggtcg tcccacaccg ccaccagcga ctctccattg
     5821 cggatatgga ggcgtcccat gttccggatc agaattgcgt tctccccggt aaggatatct
     5881 ttatggccgg tggaccagga tgcaactaca ggctttccac aggccatgta ttccatcagg
     5941 accagattgg tgccaccttc gcaacgattc gggaacaggc cgacgtccgt ctgacggtgg
     6001 aattgcgcca tgagcgcgtt cggccttggc ggcagcgcaa tgacccggct gacgtcgatg
     6061 ccgttgtcgt tcaggacctg gttcatggcg gcaaagtaat ccgtggagga cggcgcaaag
     6121 cggatatagg gagacgaccg catcgtgtcc aggctgaaac tccacgggtt gtaccaggag
     6181 tggaccagca tcacgtccgg gtgccggtct tgcagaactt tgtaggcgcg gatggcgaga
     6241 tcctgccctt tgcgaaactc gaattttccg cccgagaaaa caacaaaccg gtcggagaag
     6301 aggtctttgt catacggcgt ggggtgaaag atctgcggat cgatgccctg gacaatggtg
     6361 gaaacattct ccaggccatg tgcttcgagg acatctttac accagctcga tccggtgacg
     6421 acaacatcga actgggtccg ggcattcccg atcgattcgg ccggcaggat attctcttcg
     6481 aaaaaggtat acccgatttg cggctggccg cggaggtccg ggcggcacgg catcagatct
     6541 ttgcccgtga tgccttgaag cagcggagcg gtgccggtga actcggaagc ggacgggagg
     6601 agtgtttgta gaaaccggtc ctccatctcg ttgccgaccg tctggaaagg atccgtaatc
     6661 aggtgaacgg tagtgaggcg cgccatctcc cgcgcgatat acctgccgca gattccccag
     6721 ccgtgcgtgg aacctacggg cagagcgagg taaagggtgt cgcggccgtt ccgcccggtc
     6781 atagaggaga tagtgcccgg ccgccaggga gcttctcaga aaaaaccccg gccagcaacg
     6841 agtgcgggcc ggggccaagt tgaagttgga ctcgtgagaa attagccacg aagcaggctg
     6901 aggaccgctt gcggagcggc gttcgcctga gccatggcgg cgatggaagc ctgctgcatc
     6961 acctgggctt tggtcaggtt tgcggcttcc tctgccacgt cggcgtcgcg gatacgggac
     7021 tcggcagccg agaaaccgga gatctgcgat tgtgccagcg tgatggcata ctgcaacttg
     7081 ttttgtccgg tacccacccg gccctggacg gtgccaagtt gctcgaccgc cgccgtgatt
     7141 ttcttgatgg cggcttcggc agccgaacca tctccgctgc tggcggcagt ataggcaacg
     7201 gcgcccgtgg tagtgccggc tttgaaggca acgcctgcgc ccacattggc cacaacgctc
     7261 gcactgaatt cttcggcgct ctgcaatgag atccggttgc cggcggcatt tgtaatcgcg
     7321 tatacaccgg agccgcccaa ctgctggttg atatacgaca cggcagcgcc aatcgtggcc
     7381 gcattggtgt cgttaagggc cagcgtatag gtgcccttct gcgtggtcac aagagcggtt
     7441 tcactctcgg tggctgacat tccaacgaaa tccgcctcgg ccataacgct gttcaggctc
     7501 gtgttggcgt cggcagatac cgcatcggtc gcaatggaat tcgccgccgc ggcaacgctg
     7561 acgctgaagg ccacgccgct gttcagacgg agcttgccgt cgccgccgat ggaagccgca
     7621 atcccataat cggagagctg ctcattcaat tttgcaaccg cttcctggcc gctcgcggcg
     7681 tcatgcgtat aggtgatggc aaccgtcgag ttctggttat tgcggtacat gtggaacgtc
     7741 agggtagcat ccccgctgaa ggtccccgct acgttatcca gccggacatt gttaccagcg
     7801 agggaaacac cacctgccgc aatattggtg ccgctcaatc cgagtttgtc gtaggtcacg
     7861 acgttgctgt ccccgctgag atcgacagac actttggagt tggcctgtgt ctcgccgcca
     7921 ccgccagtgt agacgtcgat gactttgttg aacgtgccgt tgctgtcgag cccgatgttg
     7981 gccgcctgac ggtcgatttc attcaatagc gcctgatact cgttgttcaa cgtctcgcgc
     8041 ttcccggtga aggtaacgga agccgactgg gttgccagcg tcttcagccg gtccagcatc
     8101 tttgagatgt tgttcaggcc gccgtcgatg atctgcaatt ggctcaagcc gtcgtttgcg
     8161 ttccgtacgc cctgcatcaa ctcggccgta tcggagcgaa acttgttcgc aaccgccagg
     8221 ccggcggcat cgtcgccgga ggaattgatg cggtaaccgg aggtcagccg ctggatcgta
     8281 cgcccttgga aatcgctgtt cacccgcagg ttttcctgcg cgatcagcgc gtttacgttg
     8341 gtgttaattg agatcgccat ataaggttca gtccttttgt tcagtgttgc tggcttcctg
     8401 ccagccgcgg ccatcctttg ccgtcactga actcatcggt gcagccattg gatccttgaa
     8461 ccaaagtttg aagttgtttt actacctctg ttattacgct tgcccaatcg ccccaggccg
     8521 gctgccggaa caggcgcatc gacggatacc atggcgtgcg gtttccttcg atgccccagc
     8581 gccagtcggg gttgtaacag agcagggtcc acaccggcac accgagcgcg cccgccagat
     8641 gcgccgtcat cgtgtcggct gtgatcacca ggtccaggtt ccgcatcgcc gcggcggtat
     8701 cgcggatgtc cggctggcgg ccgctcagat cgaccatggg caggccgccc ctttgcacct
     8761 cacttgcttc gacacccatt tgcaggttga acagcgtaac gcccggaaga tccagcggcg
     8821 ccagggattc gaggggcacg gagcgatggc ggttgttggt gttctgcaca tttcccgtcc
     8881 acaccagtcc gaccttcaat ccggaatagc cggcgatgaa ctcgtgccac tgtgccaggc
     8941 gggcgctatc caccgagaga taaggcgccg ctccggcagg cgcaccggcg cctgtttcga
     9001 gcactctggg caggcttagc agcgccacgt gaaaatcgaa gtgggggata tcggcgcctt
     9061 tagcgatcat ctggtccgcg gcaatcctgc tctccgcaaa caaggcgacc agccccggct
     9121 ggcattcgag cacgatcttc gcaccacgct cgcgcaccgc atccagatac cggacaaatt
     9181 gaatgctgtc ccccaacccc tgctcggcat aaacgaggac ggtttctccg ttgagcaccc
     9241 gtccgtccca ttcaggctgc ggaaaagtgc gcccgggcac gtcgttgcgt ttccagcgcc
     9301 attcatattc ccgccagccg cgtttaaaat cgccggtcag cagtagaaca atggcccggc
     9361 cgaatcgcgc actcacgtca tccggcgcca actctaccgc tcgattgagc cactgaagcg
     9421 cccctttata gtcgtggcgg cacagggcgg catagccaag attgcaatac gcctctacaa
     9481 agccgggagt cgcctctaac gcctcctcca atgctttcga agcctctttg aagcggccct
     9541 gttcgcatag cgcccggcct aatgcgctat aggccgcgcc ataacccggc ttcagccgca
     9601 aggcttcccg ataacaggat tccgcttctt cgaagcgatg ccttctcccc agcaccagcc
     9661 cgagattgac ctgggcttgc ggtaaggccg ccttcaggcg gacggcgcgg cggcagtgaa
     9721 attcggcttg ctcgagttct cctttttcaa gaagaatggc tcccagatta gaatgtgcct
     9781 ccggataatt gggctggagc gaaagcgcct gccggcagaa ttgttccgct tcgtcgagtt
     9841 tcgactggac ccgcagcacg gcccccagat ttgagagtgc ctgcacgagg ctgcgcttgc
     9901 tctcgatggc cttccggtag tgccgctccg ccgtttgatg gtcgcaacgg tcgtagtaga
     9961 ggttcccgag gttcatgtgc gcctcagccc catccggatt cagttccagc gcttttctaa
    10021 aggaggcttc cgcaccgccg gcatccccga gcgagcgcaa agcatttccc agacatgtgt
    10081 aggtggcggc gcaaccgggt ttgagttcga tcgacttccg gtaccagaag atggagtccg
    10141 cgggacggtc caactcttgc agcgcattgc ccaggttggt gtaggagtcc gcaaaatccg
    10201 gacggcagcg gatcgcctgt tcatagcagt acactgcctt ctcgggcctg cctattgcca
    10261 gcatcacgtt tcccaggttg ttgtgaaaca tgggaatccc gggctccagg cggattgctt
    10321 tggcgatgtg cgaagcggcc gcgacatgct gccccgcttc gtagaacagc attccgagca
    10381 gatgccacgc atcggcattt tcgggattct cgttgagaat gccgcgatag agcgcttccg
    10441 cctccaggcg gcggccccgc ttctgttgtt ccagggcggt ggtgagttga tcgagtatgg
    10501 tggtcatccg aagacaggca gtgcacctcc tcagaactat cggcgaagaa ttacgaaatc
    10561 ttgcggttgg gggtgaaacg gcttacgcgt cgaattccca ggaggagccg gcgggcacag
    10621 ccgcggcggg atacggcgaa tggctgtggt tcaattcctt ccattgcgcc gagtggtgcg
    10681 cccgcgctgc ccgggcggct ttcaatgccc actcgagcag ttgccgggaa cagatctcca
    10741 gggtgtgcgc ctcgggggaa tccggggcga gcgcccgcgc cgctgcctcc agctccgtct
    10801 ggtaggtagc cgcaagatca cgagcgtccg cataacgacc ttcggcgacc gctttccgta
    10861 agcgagccgc ggtggcggcg ggtttcttcg ccatcagctc ataccccggc tggcgactcg
    10921 catcgactcg aacatgttcg tgaagtaggt tacctgctgt tccagcatcg caattaatgc
    10981 atcggcttca gccatctgcg cctgtagccg cgtctccagg ttcccaatgc gttcctgttc
    11041 agcggcgatc cgcgcatcct gggacttgag agtgttcgat aaggaagaga tggtgagtcc
    11101 gagtacactg ttgtccgagc tctggagacc atttacggcc gtggcggccg attgaagaaa
    11161 gcctccttcc gccgtgctgc cgaaaaaagc gagcatcttc tcgaactggt cttttgtgtt
    11221 tgcggagaac gttgccgaat tgaggctcag tttgcccttg tcgtcgaact ccaaaccgag
    11281 atccgcgagc gaggagattt cacctgttcc ggaagaatag gaagcggtgc tgcggagcgt
    11341 gtccgcaact gtcgcaatga tactgtgccc cttcagcacg ccattgccct gtccgcggtg
    11401 cacatccaat tcagtgaccg cggcgttgta ggcattgaca aacgacgata acgcgttact
    11461 gacagagttc gtatcgcggg atacggtgat ggtgaccgga tccgccgtgc tctcctgctg
    11521 tttcagcagg tctactgtaa ggccgggtgc tatatttacg gtccgggagt cgctggtcgc
    11581 caccgtggag gaaccattga cgatgtattg ggctggagct cccgctgtca attggtcgag
    11641 aagatcgttt gtgccatcgg taagctggat ctgatccgta gaaagtgttt tcaactgaat
    11701 cgagattcta taatccggcg tactgccgcc aacgtttaca acagaagcct gaacgtcgcc
    11761 gccggcttgc ttgttgattt gctccaccag cgcattcagg ttgtttttac cggcaagatc
    11821 gatatcgtaa gatgtccgga ccgcgccctc cccgtccagg atctttagtt gataggtgct
    11881 ggaagcggtg aggttttctt tcgaggcatc ctttacctga agcgtaaccg ggtcggcatc
    11941 ggcccagttt ttccccattg tgttggtata ggcacccagc tcctttacct gaaccgtgta
    12001 ggtgccggcc aggactcctt ccgccaggtt ggccttcaca atagaggtat cggagacgga
    12061 gctcttatac gagttcagcc ccagggatgc gctcaaagtg gaaatggccg attgcacctg
    12121 gtcgaccttg gttttgaggc tgcttgctgc cgaggactgg ctgttcagca caccccgctc
    12181 gctctgcatc tggctaagcg gcagtgaagc gatggctaca gcgcgatcta tcacggattg
    12241 aaaatccgat gcatagcgac tggaaccagt gaatatggtc gaactcatac gcctccccat
    12301 gacgtatcgg cagagcaggc gttgagcttt aataaatgcc accgcttcct gacggtcgcg
    12361 gctct
//
LOCUS       DASTMK010000027        14212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_193586, whole genome shotgun sequence.
ACCESSION   DASTMK010000027 DASTMK010000000
VERSION     DASTMK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193586"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="VFQ79_00900"
     CDS             complement(<1..1657)
                     /locus_tag="VFQ79_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138231.1"
                     /translation="MYNVLIRFGVLLYLFASFQAAGQPPVEAAATLIVKVQDSNQSPV
                     AGATVHLGEPGRGRRARHRHGWHHGGGPGCAGARCAQVTDAKGEAVFQGLTAGGWPVV
                     ASAESFFPNEAQIDLVTGQETVLTIELQRRPVVSETVVVTGSGTESLVQEAPIRTELI
                     TPQVVERQVKTTLAEAFQATISGVRTEMNCQNCGFMQLRMNGLEGPYTQILEDGLPSY
                     SGVTAVYGLEQVPAAFLDQIEVVKGGNSALYGPGAVAGVVNLIRREPRENHFRIDTLT
                     GWHFGRPEQQLGASAQLAQLPAGFAGDFYYRGSNRVAIDRDRDGFSDIGKRQLQSGGF
                     GLFRRFLDGKARLSATGTVADEFRRGGDQLDRPAHETWITEQIESRRYSTAIGWNHAL
                     SAKTYYNLRGSHAYYGRQTYYGAGRDPNAYGNTRNPLWVGDAQLAHQAGRHSLLGGYQ
                     ISWEHVEDNAPAYGRTYGGTFQNHGVYLQDEWRATTRVSLIGGTRFDKSNQIDHWIVS
                     PRVGTKFGITDNLVWRTTLSTGFRAPAVFDEDLHIAQVGGEGFV"
     gene            1843..2007
                     /locus_tag="VFQ79_00905"
     CDS             1843..2007
                     /locus_tag="VFQ79_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138232.1"
                     /translation="MDLDLSRFEAQTLEVAEGAHPARLRVVPVERTANDNADGQQDDC
                     AQRTYKLAAT"
     gene            2183..2638
                     /locus_tag="VFQ79_00910"
     CDS             2183..2638
                     /locus_tag="VFQ79_00910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0138233.1"
                     /translation="MSRGHAGGRPLAPDFRLLDMQGVRRKLSDFRGKVVLLNFWATWC
                     AHCKSEIPALNRVHRDYQARGVAVVSVAMDDRGWAAVTPFVTEFEVDYPVLLGNAAVA
                     RQYGGLKTLPHTLFLDRDGHIVASHEAALSEAHLRKIAETLLAESRETE"
     gene            2676..4469
                     /locus_tag="VFQ79_00915"
     CDS             2676..4469
                     /locus_tag="VFQ79_00915"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HEU0138234.1"
                     /translation="MKFSRRSFVHAASTVPAAFAQPASTFVSSNPSNQPEGKTQLTVT
                     SDGVIHAETPTLLAKIEKGFLTSLKSKASQEEYLVGFDTGKFDALQLVYARGETVDVG
                     EQKFGKVETRKISDHRAEVIFQNWDGDGIMTVSVDPASGDLLIEPSAFSSRPGVRACR
                     WRLKGLRRDLKLVAPFFQGVSLSLDDSLIRSSHWDWPHRWEAGLAILQASAGGFSVHT
                     QDNRYRYKALHVGSEADPYMIGLDTEAYGPLDANLAAGGLTWRINVHQGDWKVPAERY
                     RNWLWETYSLAKEEQRREPWLHSLAMAISWCPGDIAVLEGLARRVDPGKVLLHFSNWR
                     TDAYDENYPTYIASENAAAFLARALEMGFHVMPHFNIVETDPNHPVYAQVRDFQYRDI
                     ETKKLYGWSWVNARAIGVPESNASRLNHRREKVMVKIHPGLAAWRSMLCSRIQEAARQ
                     HALREVFVDVALNTFNLHNCLVENTTSTEGVNRLVHQIADLGLSVGAEGLNETFFQGL
                     SFAQVHLFRNPRTTMEGLERTGGCPLNHFLFGKLCRSFGYSGLGGRNADEELRARVHE
                     ENGALPTITIRSAGEITNPTPAVKRALDRAR"
     gene            4558..4893
                     /locus_tag="VFQ79_00920"
     CDS             4558..4893
                     /locus_tag="VFQ79_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0138235.1"
                     /translation="MSRPSDLVQGTLDLLVLKLLALEPLHGWAISQRLRHVSDDVLKV
                     SDGSLYPALHKLEQEGWIKAEWRVTANGRRAKFYRLTRLGRAQLEKETADWERLAGAI
                     FQVVRLDEA"
     gene            4897..7602
                     /locus_tag="VFQ79_00925"
     CDS             4897..7602
                     /locus_tag="VFQ79_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138236.1"
                     /translation="MRLEHWFYIVPSRLRSLLRRSQVERELNEELRFHVDNQIDEYIA
                     RGLTPEEARYAALRAFGGVEQRKEECRDMRQVRALDELIQDVRYAIRVLWKSPGFAAT
                     AVATLALGIGANTAIFSVAHALLLKPLPYANPDELYAVEMHLPQYSDKYPSLPVRVRE
                     LIGWPQASTGVSQISAVKGGNFNLTGSGEPERIGAVAVSTNFFSLVGVQPRLGRGFIA
                     GEQQSGHDNVLVISHEFWVRRFGADPGVLNQRISLDGLPYQVVGILPPHFLFPTGKQL
                     HPLVPLPPRIDVWKPLALSPQELRRRDTDWQYGALVRLKTGAAAEQVRQQLEAIAQRN
                     IQQDLPNIPLDVRVRLIPMREVFSGNIRLGLILVLCAAVLLLLIACVNIASLLLARAI
                     HRRNEFAIRVALGAGRRRLIRQSITEGLVLSTLGGAAGLLLAVWGTRILIARAAAALP
                     VFQEVGANASVLAFTLGVVLITGLGLSLAPAVQLFGVGMSGGLAAGAGARAVSTGTAA
                     ERLRRLLVGFEVALSAALLMTAGLLVHSFIRLMAVDKGFQVENIVCMNLTLGQEYQNS
                     ALRLSFYRDLLERIRALPAVTAAGGTSVIPLTDAPGGSSIYLESDTQLDEANLKRPTA
                     SYSVITPGYFAAVGIPLRAGRFLGEQETQPAALISEKLARALWPAESLTAVVGRRIRN
                     GSPDASLWTVVGIVGDVRLSALDRASVPQIYGPYHQSPIGAMSIVVRTASGDVRALAS
                     ALRDEVRQRDKNLPVTNLRTMSEIVAASVAQRRFQMSLVSLFAIAALALVLVGIYGLV
                     SYSVACRTRDIGLRIALGASREEILRSVLLTGLRPVVIGLVCGIAGTVLGARALRSLL
                     FGVGPLDPVAVGSVAVILLAAAVVACYVPARRASGLDPATALRCE"
     gene            complement(7635..9677)
                     /locus_tag="VFQ79_00930"
     CDS             complement(7635..9677)
                     /locus_tag="VFQ79_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU0138237.1"
                     /translation="MSELVRSLPWRAEAADQPESLLSREWLVTNGLGGYASGTLAGVL
                     TRRYHGLLIAALEAPYGRRVMLPDVAESIEENGDVIDISGEEPVSDTLSLHGSKYLTE
                     FRLEEGLPTWRFEFKGVVLEKKLVLLHGQNTLHVTYRLLQGDRTLSLHLRPSLQFRPH
                     EAPVSMPLREPFIVTTIADEYEFSSAREDPALRMKVLGPYPGFTFDQRHIQQILYRIE
                     ESRGYESHGDLWSPGYFHVELSPETNEATLIASTEEWHIIRALSPAEALAAERKRRRA
                     LLAKADPRAKTGMGAELVLAADQFVITPRGRSQDVARAHASGDEIRTIIAGYHWFTDW
                     GRDTMISLEGLTLSTGRDVEAGWILRTFAYYIRDGLIPNMFPEGENEGLYHTADATLW
                     FFHALDRYMEYCDDRITLALLLPKLRDIVDHHLRGTRFGIGVDPRDGLLRQGQEDYQL
                     TWMDAKVDDWVVTPRRGKAVEINALWYNALRLLERWEREESGDAAAMPYAEHAERARR
                     SFNERFWYEEGAYLYDIVDCENGGNDSALRPNQLFSFSLRYPVLDESRWERVLHVCQE
                     KLLTPFGLRSLAPGSPEYKLKYYGDLRSRDAAYHQGTVWAWLIGPFINCWLRVHPGDR
                     KGARRLLEGFSTELDRFGVGTIAEIFDAEEPYHPRGCIAQAWSVAEVLRCWVETAE"
     gene            complement(9670..11658)
                     /gene="treZ"
                     /locus_tag="VFQ79_00935"
     CDS             complement(9670..11658)
                     /gene="treZ"
                     /locus_tag="VFQ79_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEU0138238.1"
                     /translation="MQTEKFERRRTARQLPAGAEVVPGGVEFRVWAPGKTRVEVVIEG
                     GRDPAVHQLEPEQSGYFRGIVQGASAGTLYRYRLDGEGPYPDPASRFQPDGPHNPSEV
                     IDPSTFEWTDSNWAGVSLEGQVLYEMHIGAFTPEGTWEAARRQLGELAALGITVLEIM
                     PVSEFPGRFGWGYDGVHLFAPTRLYGRPDGFRQFVNDAHAAGMGVILDVVYNHLGPDG
                     NYLGRFSEHYFTDRYTTDWGEAINFDGKNCAEVREFFLSNAACWIREYHLDGLRLDAT
                     QDIYDSSQGEHILAEMARRTREAAKGRTIILIGENEPQETRLIRPPEQGGYGLDALWN
                     DDLHHTAMVTLTGRNDAYYSDYRGSPQEYISAFKYGYLYQGQRYKWQEQRRGTPTAGL
                     KHSAFVNFIQNHDQIANSARGQRCHALTSPGQYKAVTAVILLAPGTPMLFMGQEFAAS
                     TPFLYFADHTPELSKLVREGRAEFLAQWRSLGLDEMQSVFADPAAEDTFKRCKLNSEE
                     RRTHAEIYALHRDLLKLRREDPVFRRQGEKGVDGAVLGPEAFLIRFFGKAGDDRLLVV
                     NLGLDLHLSPAPEPLLAPPEDRTWSILWSSEHPAYGGTGTAPLETEENWKIPGHAAVV
                     LGPAESEPWVRGLGSFTKKRGFPSLRALAIREGKAGNE"
     gene            11782..12213
                     /locus_tag="VFQ79_00940"
     CDS             11782..12213
                     /locus_tag="VFQ79_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HEU0138239.1"
                     /translation="MKGLCLCFFLGLLAISNSAIAQTSGEKVTAAQKAAESWLVLIDG
                     DKYGESWEQAAETFRSAVAKEKWEGMVGSVRDQIGKSKSRKLIRAEYTTKLPNAPAGE
                     YVLIQYDSSFEKADAAIETIVPMREKNGEWKVSGYFVKPKQ"
     gene            complement(12224..13759)
                     /locus_tag="VFQ79_00945"
     CDS             complement(12224..13759)
                     /locus_tag="VFQ79_00945"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEU0138240.1"
                     /translation="MNGASRPRPCSRERAELEAILASPLFQRAPHLSKLLAYLCERTC
                     AGEADSLKEYSIATEAMGLPPDFSEKKGAVVRVEMHRLRKRLQQYYETEGSGRSLRIE
                     LLPGQYAPRFVSVRETAGLDSSAPKDLELPPPRRRRSFRLGKNAVWGAVALVIPAAFY
                     LAGGLGEREKEPQAPQSPAKSVPAPAAGFSGGAIRILAGFQQARYVDRMGNTWQSDSF
                     VTGGYPSHYEGETRPVYVARAPDPGLFQSYRSGDFTYEIPLAPGSYQLALYFMEHKFG
                     EGTTLGGGEESRSFDVYLNDELLLWRFDILADIGEPGMAAMRVFRDVTPAADGKLHLR
                     FQSVHDRALLNAIEILPTPDRKTIPVRIVAQDRPATDAHGEVWELDRFYRGGRLAPAR
                     ADSGAPNSMLLGGERFGNFSYVIPVDPRGRYKLSLYFMESFFGAGNPGESDRRRRTFD
                     VYSNGRVLLDNFEILAEASPRQSLVKTFHNLVPTPSGKLIIGFQPRGHHALVNAIEVV
                     EER"
     gene            13918..>14212
                     /locus_tag="VFQ79_00950"
     CDS             13918..>14212
                     /locus_tag="VFQ79_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0138241.1"
                     /translation="MRRILAALLFTSFPALSQVTSGTITGVVKDPSDSTVVDAKVEAR
                     NPATGLVRTVRTSGEGVFVVPNLQPGEYTIAVEAPGFQTLEKTGIFLAAADRLN"
BASE COUNT         2775 a         4262 c         4305 g         2870 t
ORIGIN      
        1 atacgaagcc ttcgccgcca acctgcgcga tgtgaagatc ttcgtcgaaa acagccgggg
       61 cacggaaacc ggtggacaat gtcgtgcgcc acacaaggtt gtccgtaatg ccgaactttg
      121 ttcccacccg agggcttaca atccagtggt cgatctggtt cgacttgtcg aatcgcgtgc
      181 caccgatcag ggagactcgg gtagtcgccc gccattcgtc ctgaagatac acgccgtggt
      241 tctggaaagt gccgccgtag gtccggccat aggccggtgc attgtcctca acgtgctccc
      301 acgaaatctg gtagccgccg agaagggagt gccgtccggc ctggtgcgcc aactgtgcgt
      361 cgcccaccca cagcgggttt ctcgtattgc cgtaggcatt gggatccctg ccggccccat
      421 agtaagtctg gcgcccgtaa taagcgtggc tcccacgaag gttgtaatac gtctttgccg
      481 agagggcgtg gttccacccg attgctgtgc tgtaccgccg ggattcgatc tgctcggtaa
      541 tccaggtctc gtgagccggt ctgtccaact ggtcgcctcc acggcggaac tcatcggcaa
      601 ccgtgccggt cgcggaaagc cgtgccttgc cgtccagaaa gcggcggaag agcccgaatc
      661 caccggattg aagctgccgt tttccaatgt cggagaatcc gtcgcggtcc cggtcgatcg
      721 ccacccgatt gcttccgcgg tagtagaaat cgcctgcgaa accggctggt agttgggcca
      781 gttgcgccga ggcgccgagt tgctgttccg ggcggccgaa gtgccagccg gtaagggtgt
      841 cgatacggaa gtggttctcc cgcggctcgc gccggatcag gtttactact cctgccaccg
      901 caccggggcc gtacagcgcc gagtttcctc cttttaccac ttcgatttga tcgagaaacg
      961 ccgcggggac ctgttccaat ccatagacgg ccgtgacgcc cgagtagctg ggcaaaccgt
     1021 cttccagaat ctgcgtgtag ggtccctcga gcccgttcat tcgcagttgc atgaagccac
     1081 agttctggca gttcatctcc gttcgcacgc cggagatcgt cgcctgaaac gcttcggcca
     1141 gggttgtttt aacctggcgt tcgacgacct gcggagtgat gagctcggtt cggatgggag
     1201 cctcttgaac aaggctttcc gtacctgagc cggtcaccac gacggtttca gaaaccaccg
     1261 gacgccgctg gagttcgatg gtaaggacgg tctcctggcc ggtgacgagg tcaatctgcg
     1321 cctcgttcgg gaagaacgat tccgccgagg ccactaccgg ccaaccaccg gccgtcagcc
     1381 cctgaaagac cgcctcgcct ttggcatcgg ttacctgcgc gcagcgtgcg ccggcgcagc
     1441 ccggaccacc cccatgatgc cacccatgac ggtgtctcgc gcggcggccc cggccgggct
     1501 cgcccagatg caccgttgcg cccgccaccg gcgactggtt cgaatcctgg accttgacaa
     1561 tcagagttgc agcagcctcc acgggcggct gtccggcggc ctgaaagctg gcgaaaaggt
     1621 aaagcagaac accaaaacgg atgagtacgt tatacacaac tgatcctcct ctcttaacaa
     1681 cggaactctc gcttcgataa ggccggagcc aggtcgaaac acttacgcgg cgaagaggaa
     1741 ggatggtggc gcacgcccgg gatgggggct ctcaaaggat gaagagggcc tgaccgatgg
     1801 cgtcacggcc gggcaccaca ttatgagagc ggggacatcg cattggatct ggacttgagc
     1861 cgtttcgaag cccagacttt ggaagtggca gaaggggcac atcccgcccg cttgcgggtc
     1921 gtcccggtgg agcggactgc aaacgataat gctgacggcc agcaggacga ttgcgcacag
     1981 cgcacctaca aactggcggc gacgtaatct gcaactcatt tgcaactata aaatatcata
     2041 agatttcaat acctgcaagt cattaatttg gatacactgg aaacggagcc gcgcgcgcca
     2101 gcacgcggtg ccgggatact tgatgtttgc cactgccaca tcgcggcgat ccttgttccg
     2161 cgcgcttggc gccctgttcg ctgtgtctcg cggacacgcc ggtggacggc cgctggcccc
     2221 cgatttccgg ttgctggaca tgcagggcgt caggcggaaa ctgtcggact ttcgtggtaa
     2281 ggttgttctg ctgaactttt gggccacgtg gtgtgcccac tgcaagagcg agattcccgc
     2341 actgaaccgg gtgcatcgcg attaccaggc gagaggtgtt gccgtggtaa gcgttgcgat
     2401 ggacgatcgg ggatgggcgg ccgtgactcc cttcgtcact gaattcgaag tggattaccc
     2461 ggttcttctc ggcaacgccg cagtggcgcg ccagtatggt ggtctcaaaa cgctgcccca
     2521 cacattgttc ctcgaccgtg atggccacat cgtggcgagt catgaagctg ctttatcgga
     2581 agcccacctc cgcaaaatcg cagagacctt gctcgccgag tcccgtgaaa cggagtaggg
     2641 gactggccgc tgtagggctg ttattctgag tggcgatgaa attctcacgc cgcagtttcg
     2701 tccacgccgc cagtaccgtc cccgcggcct tcgcccaacc cgcctctacg ttcgtctctt
     2761 ccaatccgtc taatcagccg gaagggaaga cccaactcac tgtcaccagt gacggagtga
     2821 ttcacgccga aacccccacc ttgctggcga aaatcgaaaa gggattcctc acttcactga
     2881 agagcaaggc ctcccaggag gaataccttg tgggcttcga taccggcaag ttcgacgctc
     2941 tccaactcgt ctacgcgcgg ggcgagacgg tagacgtcgg cgaacagaag ttcggcaaag
     3001 tggaaacacg aaagatttcg gatcatcggg ccgaggtgat ctttcagaac tgggatggag
     3061 acggcatcat gacggtgtcc gtcgaccccg cctcgggcga tctgctcatc gaaccttccg
     3121 ccttctcctc tcggccgggc gtccgcgcct gccgctggcg cttgaaagga ctgcgccgcg
     3181 atcttaagct ggttgcgccg ttctttcaag gcgtgtcgct cagtctggac gattccctca
     3241 ttcgttcctc ccactgggac tggccgcatc gctgggaggc gggactcgct attcttcagg
     3301 cctctgccgg cggattttcc gttcacacgc aggacaaccg ctatcgctac aaggcgcttc
     3361 acgtcggcag cgaggctgac ccgtacatga tcggactgga cacggaagcg tatgggccgc
     3421 tcgatgccaa cctcgcggcc ggaggactta cgtggcgaat caacgtccac cagggcgact
     3481 ggaaggtccc ggcggagcgc tatcgcaact ggctctggga aacgtactcg cttgccaaag
     3541 aagagcagcg ccgggaaccg tggcttcatt cgctcgccat ggcgatttcc tggtgcccgg
     3601 gagacatcgc cgttctggag ggccttgcca ggcgtgtgga tcccggaaag gtgttacttc
     3661 acttctccaa ctggcgcacc gacgcctacg acgagaacta cccgacgtac atcgccagtg
     3721 aaaatgcggc ggcgtttctg gcccgcgccc tcgagatggg ctttcacgtg atgccgcatt
     3781 tcaacatcgt ggagaccgac cccaaccacc cggtctacgc gcaggtcagg gacttccaat
     3841 accgcgacat tgaaacgaag aagctctacg ggtggagttg ggtgaatgcc cgcgctatcg
     3901 gagtgcccga atcgaacgcc agtcgcctca atcaccgccg cgaaaaagtc atggtcaaga
     3961 tccatccggg gttggccgca tggcgctcca tgctttgttc gaggattcag gaagcggcgc
     4021 ggcaacacgc actccgggag gtgttcgtcg acgtcgccct gaatacgttc aatctccaca
     4081 actgcctggt cgagaatacg acctctaccg agggcgtaaa ccgcctagtc caccagattg
     4141 cggacctcgg cctctccgtc ggcgccgagg gattgaacga gacattcttt caggggctct
     4201 ctttcgcgca ggtgcacctg ttcagaaatc cgcgcaccac gatggaggga cttgaacgaa
     4261 cgggcggctg cccgctcaac cattttctat tcggcaagct gtgccgctcc ttcggatact
     4321 ccggtctcgg cggccgcaac gcagatgagg aactccgcgc ccgcgtacac gaagagaacg
     4381 gggcgctgcc aaccattacg atccggtccg ccggggaaat cacaaacccc acgccagcgg
     4441 tgaagcgggc gctcgaccgc gcccgataaa ttgcagatag aggatataaa gacaaattgt
     4501 tcttgaattg agaggcggag cgggtgtaag ataaagctcg gtaaccgagg gaagagcatg
     4561 agcagaccat ccgatctcgt ccagggaact ctggatttgc tggttctcaa gctattggcg
     4621 ctggagcctc tgcacgggtg ggcgatcagc caaaggctga ggcacgtatc cgatgatgta
     4681 ctcaaagtca gtgacggttc actctatccg gccctccata agctagagca ggaagggtgg
     4741 atcaaggcgg aatggcgcgt cactgcgaat ggccgccgcg ccaagttcta ccgcctcacc
     4801 cgcctcggcc gggcccagtt ggaaaaggaa accgcagact gggagcgcct ggcaggtgca
     4861 atcttccagg tggtgcggct cgatgaggct tgagacatgc gcctggagca ctggttctat
     4921 atcgttccgt cccgtctgcg ctcgctgctc cgcagaagtc aagtcgagcg cgaactgaat
     4981 gaggaattgc gtttccacgt tgacaatcag atagacgaat atatcgccag aggactcacg
     5041 cccgaggagg ctcgatacgc ggcgcttcgt gcgttcggtg gcgtcgaaca gcgaaaagag
     5101 gagtgccggg atatgagaca ggtgagggct ctcgacgaat tgattcagga tgtacgctat
     5161 gcgatccgag tattgtggaa gagccctggc tttgccgcta cggcagtcgc gacactggcg
     5221 cttggaattg gtgcgaacac ggcgattttc agtgtcgcgc acgcccttct tctgaaaccg
     5281 ttgccctatg ccaacccgga tgagctatac gccgtagaaa tgcatttgcc gcagtatagc
     5341 gacaagtatc caagcctccc ggtccgcgtg cgcgagttga tcggttggcc gcaggcatca
     5401 acaggcgtgt cacagatctc ggctgtgaag ggcggcaact ttaatctcac cgggagcggc
     5461 gaaccggaac ggatcggagc cgtagcggtt tctaccaact tcttttccct cgtcggcgtg
     5521 cagccgcgtc tcgggcgggg ctttattgcc ggcgaacagc aatccgggca cgataacgtc
     5581 ctggtcatca gtcacgagtt ctgggtacgc cggttcggag cggaccccgg cgtcctcaac
     5641 caacgcatat cgctcgatgg ccttccctac caggttgtcg gtatcctgcc gccgcatttt
     5701 ctcttcccca ccggcaagca actacatccg ctagtgccgc tcccaccccg catcgatgtg
     5761 tggaagccgc tggccctctc gccgcaggaa ctccgaagaa gagacacaga ctggcagtat
     5821 ggcgcgctcg tccggctgaa gacgggcgct gctgccgaac aggtccggca acaactcgaa
     5881 gccattgcgc agcgcaacat tcaacaggat ttgccgaata tccccttgga cgtgcgcgta
     5941 cgcttgatcc ccatgcgcga ggtcttctct ggcaacatcc gccttggact gatccttgtt
     6001 ctgtgcgctg cggtcctgct gctgctgatt gcctgtgtga acattgccag cctgttgcta
     6061 gcccgtgcca ttcatcggcg gaacgagttt gccatccggg tcgcgctcgg agctggccgc
     6121 cggcgattga tccgccagtc gatcaccgaa ggcctcgtgc tttccacttt aggaggtgcc
     6181 gccggcctcc tgctggcggt ctggggtacc cgtatcctga ttgctcgggc ggcagccgcg
     6241 ttaccggtgt tccaagaggt aggcgccaac gcttctgtac ttgccttcac tctgggtgtg
     6301 gtactgatca ctggacttgg ccttagcctg gcgccagcag tccagctctt tggagttggt
     6361 atgagtggag gtcttgcggc cggggccggg gctcgcgctg tgagcacagg taccgcagca
     6421 gaacggctac gccgcctcct ggtcggcttc gaggtggctc tcagtgcggc gctactgatg
     6481 acggctggtc tgctggtaca cagttttatt cggctgatgg ctgtggataa aggcttccag
     6541 gtggaaaaca ttgtgtgcat gaacctgacg ctcggacagg agtatcagaa ttccgcactg
     6601 cgcctctcct tttatcgcga tcttctggag cggatccggg cgctcccggc cgtgactgcg
     6661 gctggcggaa ccagtgtgat cccgcttact gacgcgcctg gtgggtcctc tatctacctg
     6721 gagagcgata cccaactcga cgaagcgaat ttgaagcggc ccactgcgag ctacagcgtc
     6781 atcactcccg gctactttgc agcagtcggc attcctctac gagccggccg gtttcttggc
     6841 gaacaggaaa cccagccggc cgcattgatc agcgagaagc tggctagagc gctgtggccc
     6901 gcggagtccc ttacggcggt cgttggcagg cgcatccgga acggaagccc agacgcgtct
     6961 ctgtggactg ttgtagggat agtcggcgat gtgaggctca gtgcgctcga ccgggcctcc
     7021 gtacctcaga tttatggccc ctatcatcaa tcgccgatcg gagccatgag cattgttgtc
     7081 aggacggctt ccggggacgt acgcgccttg gcctccgccc tccgcgatga ggttcgccaa
     7141 cgcgacaaga atctgcctgt aacaaacctg cgcacgatga gtgaaatcgt agcggcttcc
     7201 gttgcgcaac gacgttttca gatgtctctg gtctcgctgt tcgccatagc ggcgcttgcg
     7261 ctggttctgg tggggattta cggcttggtg agctattcgg tggcctgccg gacgcgcgac
     7321 atcggtctgc gcatcgcgct cggtgcgagt cgcgaagaaa tcctgcgctc cgtccttctg
     7381 acgggtctgc ggccggtggt gatcggcttg gtttgcggca tcgcgggcac cgtgttaggc
     7441 gcccgtgcgc tccggagtct gctcttcggc gttggcccgc tcgatcctgt ggccgtgggg
     7501 agtgtagccg tcatcctgct ggcagcggcg gtcgtcgcct gctatgtgcc agcacgccgg
     7561 gcttcgggac tggaccccgc gacggcgctt cggtgtgagt aagagggagc gccccccgaa
     7621 ctccctcgcg attgctattc agccgtctcc acccagcagc ggagaacttc ggcaacactc
     7681 catgcctggg cgatgcagcc gcgcggatga tacggctctt cggcgtcaaa gatctcggcg
     7741 atggtgccga caccgaaccg gtcgagctcg gtagagaagc cttccaacag gcggcgggcg
     7801 cctttgcggt cgcccggatg tacgcgcagc cagcaattga tgaacggtcc gatgagccag
     7861 gcccacacgg tgccctgatg ataggccgcg tcgcgcgacc ggaggtcgcc gtaatatttc
     7921 aacttgtatt ccgggctgcc cggtgccagc gaccgtagcc cgaagggcgt cagcaatttt
     7981 tcctggcaaa cgtgcagcac tcgttcccat cgcgactcat ccagcaccgg atagcgcagc
     8041 gagaacgaga acaactgatt ggggcgcaag gcagagtcgt tgccgccatt ctcgcaatcc
     8101 actatgtcat aaaggtacgc gccctcttcg taccagaagc gttcgttgaa agaacgccgc
     8161 gcacgttcgg catgttccgc atacggcatc gcggccgcgt cgcccgattc ttctctttcc
     8221 caccgctcca gcagtcgcag ggcgttgtac cagagcgcgt tgatctcaac cgctttgccg
     8281 cgtctgggag tcaccaccca atcgtccacc ttcgcatcca tccaagtgag ctggtaatct
     8341 tcctggccct gacggagcag cccatcgcgc gggtccacgc caatgccgaa tcgcgttccg
     8401 cggagatgat gatctacgat gtcgcgcaat ttcggcagca gcagggcgag cgtgatccgg
     8461 tcatcgcaat attccatgta ccggtccagc gcatgaaaga accacagcgt cgcgtcggcg
     8521 gtatgataaa gcccctcgtt ttctccctcc ggaaacatgt tgggaataag gccgtcgcga
     8581 atgtaataag caaacgtacg cagaatccac cccgcttcca cgtcacgccc ggtgctgagc
     8641 gtcaatcctt ccaggctgat catggtatcg cggccccagt cggtgaacca gtgatagccg
     8701 gcgatgatgg ttcggatctc gtcgccggaa gcatgggcgc gggccacatc ctgggagcgg
     8761 cctctcgggg taatgacaaa ctgatcggcg gccaggacga gctcggctcc catgccggtc
     8821 ttcgctctgg gatctgcttt cgccaacagc gcgcggcggc gcttgcgttc ggcggcgagg
     8881 gcttcagccg gagaaagcgc gcggatgatg tgccactcct cggtggaagc gatcaacgtg
     8941 gcttcgttgg tttcgggcga aagctccacg tgaaaatagc cgggactcca aaggtcgccg
     9001 tgggattcat agcccctgct ttcttcgatt cgatacagga tttgttgaat gtggcgctga
     9061 tcgaaggtga agcccgggta gggaccaagg accttcatgc gcagcgccgg atcttcccgt
     9121 gctgagctga actcatactc gtcggcaatt gtggtgacga tgaaaggttc gcgcagcggc
     9181 atgctgacgg gagcttcatg cgggcggaac tgtagcgaag gccgcaagtg cagactcagc
     9241 gttctgtcgc cctgcaagag gcgataggtg acgtgaaggg tgttttggcc atgcagcagc
     9301 acgagcttct tctccagcac cacacccttg aattcaaagc gccacgtggg cagcccctct
     9361 tcaagacgga attcggtgag gtacttggag ccatgcagac tgagggtgtc cgaaaccggc
     9421 tcctcgccgc tgatgtcgat tacatctccg ttttcctcga tggactccgc aacgtccggc
     9481 agcatgaccc ttctgccata gggcgcttcg agagcggcga tcagcagacc gtggtagcgc
     9541 cgtgtgagaa ctcccgccag cgttccggag gcgtatcccc ccaggccgtt cgttaccagc
     9601 cattcgcgtg aaagcagaga ctcgggctga tcggccgctt cagcacgcca tggcagcgag
     9661 cgaaccaatt cactcatttc cggcctttcc ctccctgatg gcaagcgcac gcagactcgg
     9721 gaagccccgc ttctttgtga atgacccaag accgcgaacc cacggctcag attcggcggg
     9781 ccccagcacc accgccgcat gccccggtat tttccaattc tcttctgttt ctaggggagc
     9841 cgtgcctgtc cctccgtagg ccggatgttc gctcgaccac agtatcgacc acgttctgtc
     9901 ttccggcggc gccaacaacg gctccggcgc gggagagagg tgcagatcga gcccgagatt
     9961 cacgacaagg agccggtcat cacccgcctt gccgaagaag cgaatcagga aggcctccgg
    10021 accgagcaca gccccatcca ctcccttctc tccctgccgg cgaaaaaccg gatcctcgcg
    10081 ccggagcttc agcaggtcgc ggtgcaatgc gtagatctcc gcatgcgttc ggcgttcttc
    10141 ggaattcagt ttgcaacgct tgaaagtgtc ctcggcggca gggtcggcaa acaccgattg
    10201 catctcgtcc agcccgaggc ttcgccattg cgcgagaaat tcggcgcggc cttcccgcac
    10261 gagtttggaa agttccggag tgtgatccgc gaaatacagg aacggcgtgg acgcggcgaa
    10321 ttcctggccc atgaagagca ttggcgttcc cggagccagc aggatcaccg cggtgacggc
    10381 tttgtactgt ccgggactgg tcaatgcgtg gcagcgttgc ccgcgagccg agttggcgat
    10441 ctggtcgtgg ttctgaatga agttgacgaa tgccgaatgc ttgagcccgg ccgtcggggt
    10501 tcctctccgc tgctcctgcc atttgtatct ttgaccctgg taaagatagc cgtacttgaa
    10561 ggctgaaatg tattcctgcg gcgatccccg gtagtccgag taataagcat cattccggcc
    10621 cgtcagggtg accatcgcag tgtgatgcag gtcgtcgttc cagagagcat cgaggccata
    10681 gcctccctgt tccggcggac ggatcaaccg cgtctcctgt ggttcgtttt caccaatcag
    10741 gatgatcgtc cggcctttgg cggcttcccg tgtgcgccgc gccatctctg ccagaatatg
    10801 ttcgccctgg gaggagtcgt agatgtcctg cgtcgcatcg aggcgaaggc cgtccaggtg
    10861 atactcgcgg atccagcagg cggcattcga aaggaagaac tcccgcactt ccgcgcaatt
    10921 tttgccgtcg aagttgatcg cttcccccca atccgtggtg tagcgatccg tgaagtagtg
    10981 ttccgagaaa cggcccaggt agttcccatc cggcccaagg tggttgtaga cgacgtcgag
    11041 gatgacgccc attccggctg cgtgagcatc gttcacgaat tgccggaagc cgtcaggccg
    11101 cccgtatagt cgtgtgggag cgaagagatg cacgccgtca tagccccatc cgaaccggcc
    11161 gggaaactca ctcacgggca tgatctcgag caccgtaatg cctaaagccg ccagttcacc
    11221 gagttggcgt cgcgcggctt cccaggttcc ttccggtgtg aacgcaccga tgtgcatctc
    11281 ataaaggacc tggccttcca gggatacccc agcccagttc gagtcggtcc attcgaaggt
    11341 ggatggatcg atcacttccg aaggattgtg cggcccatcg ggttgaaagc gggatgccgg
    11401 atcgggatag ggaccttcgc catcgagccg gtatcggtag agcgtgcccg ccgatgcgcc
    11461 ttggacgatg ccgcggaagt agccgctctg ctcgggttcc agttgatgga cggcgggatc
    11521 ccgtccgccc tcgataacga cctcaactct agtctttccg ggtgcccaca cccgaaactc
    11581 cacaccgcca gggaccactt cggctcccgc cggtaattgg cgcgccgtac gtcgcctctc
    11641 aaatttctca gtctgcaatt gtcctctccg cccttcatcg ggagttatta gactgccgaa
    11701 gcggaagact gatttcaaac ggataccgcg aagagaccca ctcgagggtg tgttagcttg
    11761 ggaggagtgg aggagaaccc aatgaaaggc ctgtgtcttt gcttcttttt gggtttgctg
    11821 gcaatatcga acagcgccat cgctcaaacg agcggagaaa aagtaaccgc cgcgcaaaaa
    11881 gcagcggaat cctggctggt gttaatcgac ggcgacaaat atggagagag ttgggagcag
    11941 gcggcggaaa cattccggtc agccgtagcg aaagagaaat gggaaggaat ggtcgggagc
    12001 gttcgcgatc agatcggcaa gtccaaatcc cgaaaattga tccgggcgga gtatacgacg
    12061 aaactgccca atgcccctgc cggagagtat gtgctgattc agtacgattc ttctttcgag
    12121 aaagccgacg ccgctatcga gacgattgtc ccgatgcggg aaaagaacgg cgagtggaag
    12181 gtctccggct atttcgtaaa accgaaacag tagccgccgg gcttcagcgt tcttccacaa
    12241 cctcaatcgc attgaccagg gcgtgatggc cgcgtggctg gaagccgatg atcagcttgc
    12301 cgctgggggt tggaacgaga ttgtgaaagg tctttaccag gctctggcga ggtgaggctt
    12361 cggcgaggat ctcgaaatta tccagcagta cgcggccgtt cgaatacaca tcgaaggtgc
    12421 gccgccgcct gtcgctttca ccggggttgc ccgcaccgaa gaagctctcc atgaaataga
    12481 gactcagctt gtaccggccg cgtgggtcta cgggaatgac gtaggagaag tttccaaagc
    12541 gctcgccgcc gagcagcatc gaattgggcg ccccggaatc ggctcgtgcc ggggccaggc
    12601 gcccgccgcg atagaagcgg tccaattccc agacctctcc gtgggcgtcc gtagccgggc
    12661 ggtcctgtgc aacgatgcgc accggtatcg ttttgcggtc gggggtaggc aggatctcga
    12721 ttgcatttag tagcgcgcga tcgtgcacgg actggaaccg cagatgaagc ttgccgtccg
    12781 cggcgggtgt cacgtcgcga aagacccgca tggcggccat tccgggctcg ccgatgtcgg
    12841 ccagaatgtc gaatcgccag agcaggagtt cgtcgttgag gtaaacgtcg aacgaacggc
    12901 tctcttctcc gccgccgagc gtcgtgcctt ccccgaactt gtgttccatg aaatagagag
    12961 ccagttgata ggagccgggg gcgagcggaa tttcgtaggt gaaatctccc gaacggtaag
    13021 actggaaaag gccggggtcc ggcgcgcggg cgacgtacac gggcctggtc tcaccttcgt
    13081 agtgggaggg atagccgccg gtgacaaagc tgtcactctg ccaggtgttg cccatgcgat
    13141 caacgtagcg ggcctgctgg aatccggcca ggatgcggat ggcgccaccg ctgaaacctg
    13201 ccgccggggc aggaacgctc ttcgccggag attgcggggc ttgcggctct ttctcccgct
    13261 caccgagccc tccggccaga tagaatgccg ccgggatcac cagggcaaca gcgccccaaa
    13321 cggcattctt ccccaatcgg aaacttcgcc gccgccgcgg cggtggaagc tcaaggtctt
    13381 tgggagccga cgaatcgagc ccggccgttt cgcggactga aacaaatctg ggagcatatt
    13441 gaccgggcag caattcgata cgaagagacc gccccgagcc ctccgtttcg taatactgtt
    13501 gcagccgctt gcgaagccgg tgcatctcca cgcgcaccac ggcgcccttc ttttcggaaa
    13561 aatcgggggg caggcccatc gcttcggttg caatggagta ctccttgagc gaatctgcct
    13621 ccccggcgca cgtgcgttcg cacagatatg ccagcagttt gctgaggtgg ggtgcgcgct
    13681 ggaatagagg ggatgcgaga attgcctcca actcggccct ctcgcgcgaa caggggcgag
    13741 ggcgggaagc gccattcatt ccagaagcct cctctttacc gtaacattag cgaattcctg
    13801 ttactaagat aaccatttga aaaatagacc atatttttca ttaaacggtt tcatgctccg
    13861 tttcacggta actgtatccc cgtgggaaat atcgggcgct gtccttaggc tggaattatg
    13921 aggcgtattc tcgcagcttt gcttttcacg agttttcccg ctctttcgca agttacgtcc
    13981 gggacgatta caggcgtggt gaaggacccc agcgacagca ccgtcgttga cgccaaagtg
    14041 gaggcgcgta accccgcgac gggccttgtg cgcactgtcc ggacttccgg ggaaggtgtc
    14101 ttcgttgtgc ctaacctgca acccggagaa tatacgatcg cagtggaagc ccctggtttt
    14161 caaacgctcg agaaaacagg gatctttctc gcagcggccg accggttaaa cg
//
LOCUS       DASTMK010000028         9543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_385739, whole genome shotgun sequence.
ACCESSION   DASTMK010000028 DASTMK010000000
VERSION     DASTMK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385739"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..692
                     /locus_tag="VFQ79_00955"
     CDS             42..692
                     /locus_tag="VFQ79_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU0138242.1"
                     /translation="MAQVFHPSTNTIARVTIFGGIFLLGGLVWVLAGINSSDYVTKVQ
                     VVQPQPVPFSHKHHVSGLGIDCRYCHTTVETSNFAGIPPTETCMSCHSQIWVDSPMLE
                     PVRASLRDNKSIEWTRVNDVPDFVYFNHSIHVNKGIGCETCHGRVDQMPLMVKAETLQ
                     MEWCLTCHRDPAQHIRPREQVFTMGYSYPANQKEVGQRLVKEYNVRSLIDCYTCHR"
     gene            728..3754
                     /locus_tag="VFQ79_00960"
     CDS             728..3754
                     /locus_tag="VFQ79_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEU0138243.1"
                     /translation="MAAIRARLEQARGPEYWRSLEELASTEEFDQFVQNEFRHELPRP
                     SEVNRRDLLRLMAASFALAGLSACTKQPDEKIVPYVRAPEEFVPGKPLMFATSMPLNG
                     FGTGVLVESHMGRPTKVEGNPEHPASLGAAGMFAQASILTLYDPDRSQVVTNRGAVSS
                     WLQFVGEMVKESVDQAARKGAGLRVLTDTVTSPSLISQMRRLLAQFPAAKWIQYDPIA
                     AARNSQAGPAQNAIYSFDTADVIVSLDSDFLGSGPGSVRYSKDFARRRRVLEGQQTMN
                     RLYAAEPVPSITGTMADHRLPIKASQVEALARSLAQAVGAAGSNVETGGAGTDDAIGK
                     WLAAAARDLQEHKGRSVVAAGEWQPPAVHAVAQAINQALGNIGTTVHYAAPPQPGVSP
                     MQSLRELVGEMNAGQVDLLIVIGGNPVYDSPADLGFAAAMSKVRLRVRLGLYNDETSF
                     QCHWQIPQAHYLESWGDVRAYDGTIGIIQPLISPLYGGRTAIELIAVLAGDPNRSAHD
                     LVHEYWRSQHGQGDFEAFWRKSLHNGFIADTGGKAAVQAVPAGAKETALQAPVAAGTR
                     DSQAASLELVFRPDATIWDGQFTNNAWLQETPKPITKLTWDNVALLSPATAEHLGVKE
                     RDVLELKYRGRTVRAPAWISPGHADESVTVFFGYGRSHAGKVGDRIGYNAYALWTSEE
                     PYFGSGLEVRKTGAQLVLACTQSHHAVGKAGFGGSGRTHNRNIVRSGTFEDFQKNPKF
                     PNEEEPPPPAGLNIYPNWEYRGYAWGMSIDMNVCMGCSACVVACQAENNIPVVGKDQV
                     VMGREMHWLRIDRYYKGEFDNPETYNQPMACQHCETAPCEVVCPVAATAHSDEGLNQM
                     VYNRCVGTRYCSNNCPYKVRRFNFLLYSDWATESLRLLHNPDVTVRSRGVMEKCTYCV
                     QRISEAKIEAEKENRKLRDGEIVTACQQACPTEAIVFGDINDPSSRVSKLKAQPHSYG
                     VLTELNTRPRTTYMAPLKNPNPALPTPAANEERHGD"
     gene            3744..5126
                     /gene="nrfD"
                     /locus_tag="VFQ79_00965"
     CDS             3744..5126
                     /gene="nrfD"
                     /locus_tag="VFQ79_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU0138244.1"
                     /translation="MATDRDNPTTEMEVGPVIAPGHTYSTVTDKIASIVLTRKTPRGW
                     LIGFGISFLLFMMLMAAVTYLFARGVGIWGVNVPVAWGFAIVNFVWWIGIGHAGTLIS
                     AILLLLKQEWRTSINRFAEAMTLFAVACAGLFPILHLGRPWLFYWLLPYPNTMEMWPQ
                     PRSPLIWDVFAVSTYATVSLLFWFVGLIPDLATLRDRARNRASRIVYGMLAMGWRGSA
                     VHWHRYETAYLLLAGLATPLVVSVHTVVSFDFSIAQLPGWHTTIFPPYFVAGAIYSGF
                     AMVLTLAIPIRAFYGLEDFITRRHLDNMAKVMLGTGLIVAYGYLMETFMAWYSGLEAE
                     RYMMWNRITGPYWWSYALLIICNIAVPQALWSRRIRANVFLLWILSLVINVGMWLERY
                     VIVITSLHRDFLPSSWGMYHPTIWDWATFIGTIGLFLSLLFLFIRGLPMISIFEMRTL
                     VPQARRGGSH"
     gene            5130..5666
                     /locus_tag="VFQ79_00970"
     CDS             5130..5666
                     /locus_tag="VFQ79_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEU0138245.1"
                     /translation="MAYERLYGLLAEFDNPDDLLHAARTVREKGYQYLDAYSPMPVHG
                     LSEAVGFRHTWLPLVVLIGGLAGAAGGFFLQYWITVLHYPLNIGGRPLNSWPAYIPIT
                     FECGVLVAAITAVLAMIALNGLPRPYHPLFNAPRFALASHHRFFLCIEKRDPLFDPAK
                     TRDLLESFHTRGVCEVPV"
     gene            5671..6270
                     /locus_tag="VFQ79_00975"
     CDS             5671..6270
                     /locus_tag="VFQ79_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0138246.1"
                     /translation="MAMVLPIMVLAGCRQDMHDQPRYEALERSEFFPDGRSARLPVQG
                     TVARGHLQEDRAFYTGKQDGRLVQTMPVNVDRQLLARGRERFNIYCSPCHGTSGMGNG
                     MIVQRGFRSPPAFHTDRLRQAPAGHYFDVITNGFGAMYSYASRVEPRDRWAIIAYIRA
                     LQVSQTATLADVPEKERTELEKQTEDQQGQPQGPGGQKL"
     gene            6267..7448
                     /locus_tag="VFQ79_00980"
     CDS             6267..7448
                     /locus_tag="VFQ79_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138247.1"
                     /translation="MTPAEPLPIDIGRYRTYAAIGGVAGLAVCVLGWLLDPGQFFHSY
                     LFGFMFWFGIGSGCLAVLMLHHMVGGEWGFVIRRLLETGTRTVPLMALLFIPILFGIP
                     YLYEWSHPEVVANDHLLQEKQAWLNVPFFIVRVVIYFAIWIGLAFFLNRFSSQQDVSE
                     DPALPTRRLRNISAPGIILWGLAVTFASIDWVMSMEPHWFSTMYGALYVVGQALSAFA
                     FTITVVILLSTRPPLSRVLGVSQLHDLGNLLFAFVMLWAYVSFSQFLIIWSGNITEEI
                     PWYLRRTSGGWVLLPILLIAFHFTIPFFVLLMRDVKRRANRLVWIAAWIVLMRLVDLF
                     WLIEPAYHGRALSVHWLDLAAPIGIGGLWIAWFLWQLPARPLVPLQAPQMVNGDTPHA
                     H"
     gene            7438..7878
                     /locus_tag="VFQ79_00985"
     CDS             7438..7878
                     /locus_tag="VFQ79_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138248.1"
                     /translation="MPTDHTGTDHFEVRHETKDVNIRAIVIFGIALAAVCVLVLAFLA
                     FLFGLLGKVQPEPAPTSALEVPFQPPPEPRLQVRPSVDMERLNAFQDNILNRYGWVDR
                     SAGVVRIPIDRAMEIVAERGIPKTLGAGGPEFEVPAPVPPGVKR"
     gene            7889..8710
                     /locus_tag="VFQ79_00990"
     CDS             7889..8710
                     /locus_tag="VFQ79_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0138249.1"
                     /translation="MKYLVIAVTFCAVLLANTSSNEVPQQLRGIGIDQKLNQQIPLDA
                     MFKDEYGRNVRLGDYFGKKPVVLGLVYYECPMLCNLTLNGMLRSLRAVNLNIGKEYDV
                     LNVSFNPREGTELAAAKKEAYSRRYGREGVRNGWHFLTGNEENIRKLADAVGFHYRFD
                     EKSGQYVHASGIMIVTPEGRLSHYFYGIEFPPRDVKFGLMQASNNRIGSPVDQLLLYC
                     YHYDPETGKYGLIIMNTIRIAGGLTVAGLVLMLVVLSRRNRRRKLENARTFPVLP"
     gene            8682..>9543
                     /gene="coxB"
                     /locus_tag="VFQ79_00995"
     CDS             8682..>9543
                     /gene="coxB"
                     /locus_tag="VFQ79_00995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU0138250.1"
                     /translation="MRELFRFFPDSASSISDAVDALYLWLTTVSTIMTLLIFLAIGYF
                     AIRYRRREPGEVPTPVLGSLRLEILWSVIPFLVMLSFFTWGTYLYFHSQTSPKGGMEV
                     FITGKQWMWKIQHPSGPREINELHVPIGVPVNLTLASEDVIHSFFVPAFRIKKDVVPG
                     RLNRVWFQATKPGKYHLFCAEYCGTEHSGMIGWVYAMEKHEYEQWLSGGGGEGSMATR
                     GEKLFQQYGCASCHTMETAGRCPSVRGLYGRQVLLRGGEQVTADDSYLRESILNPRAK
                     IVAGFEPIMPT"
BASE COUNT         1860 a         2663 c         2902 g         2118 t
ORIGIN      
        1 gggattcgaa gataagccgc ttccgagcct tagaactaac tatggctcag gtctttcatc
       61 cgagcaccaa tacgattgct cgcgttacga ttttcggggg catcttcctg ctgggtggtt
      121 tggtatgggt gctggccggt attaacagct ccgactacgt caccaaggta caagtggtcc
      181 agccgcaacc ggtgcccttc agtcataagc atcacgtctc tggtcttggc atcgactgcc
      241 gttattgtca caccactgta gaaacctcaa acttcgccgg aattcccccg accgagacct
      301 gtatgagctg ccactcgcag atttgggtgg atagcccgat gctcgaaccg gtgagagcca
      361 gtctgcggga caacaagtcc atcgaatgga cgcgggtgaa cgatgttccc gatttcgtct
      421 atttcaatca cagcattcat gtaaacaagg gcatcggctg tgagacgtgc cacggccgcg
      481 tcgaccagat gccgctaatg gtcaaagcgg agaccctgca aatggaatgg tgtttaacgt
      541 gtcaccgcga tcctgcacaa cacatccggc cgcgggaaca ggtgttcacg atgggataca
      601 gctacccggc taatcagaag gaagtgggac agagactggt gaaagagtat aacgtgcgca
      661 gtctgatcga ctgctatacg tgtcatcgat aatgaacgga gacagcggtc gcacaaatcc
      721 gctcgatatg gccgccattc gggcgagact cgaacaggcg cgcggccccg agtactggcg
      781 cagtctcgag gaacttgcct ccaccgagga gtttgaccag tttgtacaaa acgagttccg
      841 ccacgagctg ccgcgcccgt cggaggtgaa ccgccgcgac cttctgcggt tgatggccgc
      901 ctctttcgcc ttggccgggc tcagtgcgtg caccaagcag ccggacgaaa aaatcgtgcc
      961 ctacgttcgt gctccggaag aattcgtacc gggtaagccg ctgatgttcg ctacttcgat
     1021 gccgttgaac ggcttcggta ccggggttct tgtcgagagc cacatgggac ggcccacgaa
     1081 agtggagggt aaccccgagc acccggcttc cctgggagcg gccggaatgt tcgcgcaggc
     1141 atctattttg acgctgtacg atccggatcg atcgcaggtc gtcaccaacc ggggcgcggt
     1201 gagtagctgg ctgcaattcg taggagagat ggtcaaggag agcgtggacc aggctgctcg
     1261 aaaaggggcc gggttacgcg tgctcaccga cacggtgaca tcaccttcgc ttatctcgca
     1321 gatgcggcgg ttgcttgcac aatttcccgc cgcgaagtgg attcagtacg atccgatcgc
     1381 cgcggcgcgg aacagccagg ccgggcccgc acagaatgcg atttatagct tcgacactgc
     1441 ggatgtgatc gtctcgctgg actcggattt tcttggatcg ggtccgggga gtgtccggta
     1501 ttcaaaggat tttgccaggc gtcgccgcgt gctcgaaggg cagcagacga tgaaccggct
     1561 gtatgccgcc gaacccgtgc cttcgatcac ggggaccatg gcggatcatc gcctgccgat
     1621 caaagcaagc caggtagagg cgctcgcgcg ctcactggcg caagcggtcg gggccgccgg
     1681 aagcaacgtg gaaaccggcg gagcgggtac cgatgatgca atcgggaagt ggctcgccgc
     1741 tgccgccagg gacctccagg agcacaaagg ccgcagcgtt gtggccgccg gtgaatggca
     1801 gcctccagcc gtgcacgccg tcgcgcaagc gatcaatcag gcgcttggaa acatcggaac
     1861 aaccgttcac tacgccgcgc ctccgcagcc aggcgtgagt ccgatgcagt ccctccgcga
     1921 gcttgtgggc gagatgaacg ccggccaggt agatcttctg atcgtcatcg gaggcaaccc
     1981 ggtttacgat tctccggccg atctgggctt cgctgctgcg atgagcaagg tcaggctgcg
     2041 cgtgcggctt ggtttatata acgacgagac ttcttttcaa tgccactggc agattccgca
     2101 ggcgcactac ctggaatcct ggggtgacgt ccgggcctac gatggcacaa tcggtatcat
     2161 ccagccgctg atttcgccgc tctacggcgg acgcacggcc atcgagctca tcgccgtact
     2221 ggctggcgac ccgaatcgaa gcgcgcacga cctggttcac gagtactgga gatcgcaaca
     2281 cggccagggc gattttgaag ccttctggcg caagtcgctt cacaacggtt ttatcgcgga
     2341 caccggaggg aaggcggccg tccaggccgt tccggcgggc gcaaaggaaa ccgcgctaca
     2401 ggcgcctgtt gcggcgggta cgcgcgattc tcaggcggct agcctcgagc ttgtgttccg
     2461 gcccgacgcc accatctggg atggccagtt caccaataat gcctggcttc aggaaacgcc
     2521 aaagccgata acgaagttga cgtgggataa cgttgcgctt ctaagtccag ccactgcgga
     2581 gcatcttggc gtaaaggagc gcgatgttct tgaattgaag tatcgcgggc ggactgttcg
     2641 cgcgcctgcc tggatctccc cgggccacgc cgacgaaagc gtgacggttt tcttcggcta
     2701 tggccgaagc catgccggta aagtcggaga ccggatcggt tataacgcct acgcgctatg
     2761 gacttccgaa gagccctatt tcggttcggg cctggaggtg agaaagaccg gcgcgcaact
     2821 ggttctggcc tgcacgcaat cgcaccacgc cgttggcaag gcaggtttcg ggggcagcgg
     2881 acgcacgcac aaccgcaaca ttgtgcgctc gggcacgttc gaggatttcc agaagaatcc
     2941 gaagtttccc aacgaagaag aacctcctcc tccggccggc ctgaatatct atccgaattg
     3001 ggagtatcgc ggctatgcct ggggcatgtc gattgacatg aacgtgtgca tgggatgcag
     3061 tgcgtgtgta gtcgcctgcc aggctgagaa caatataccc gttgtgggaa aagatcaggt
     3121 ggtgatgggc cgggagatgc actggctccg catcgaccgg tattacaagg gagaattcga
     3181 caatcccgaa acgtacaacc agcccatggc ctgccagcac tgcgagactg cgccctgcga
     3241 ggtggtttgt cccgtagccg ctacggcgca tagcgacgaa ggcctgaacc agatggttta
     3301 taaccgctgc gtcggcacgc gctattgttc gaacaactgt ccttacaaag tgcgccggtt
     3361 caactttctg ctttattcgg attgggcaac cgagagcctg cggctgctgc ataaccccga
     3421 tgtcacggtt cgaagccgcg gtgtcatgga gaagtgcacg tactgcgtac agcgtatctc
     3481 ggaagcgaaa atcgaagcgg aaaaagagaa ccgcaaactc cgcgacggtg aaatcgtcac
     3541 cgcatgccag caggcctgcc cgacggaagc catcgttttc ggcgatatca acgatccatc
     3601 gagcagggta tccaaattga aggcgcagcc gcacagttac ggagttctga cagagctgaa
     3661 cacccgtcca cgtaccacct atatggcccc gctgaagaat ccgaacccgg cgctccccac
     3721 gccggcggcg aatgaggagc gtcatggcga ctgatcggga taaccccacg acggaaatgg
     3781 aagtcggccc ggtaatcgct ccgggacaca cctattcgac ggttacggat aagatcgcgt
     3841 cgatcgtcct gacccgcaag acaccgcgag gttggctgat cggatttggc atctcgtttc
     3901 tgctgttcat gatgctgatg gcggctgtca cctacctgtt tgcgcgtggc gtgggaatct
     3961 ggggtgtcaa tgttccggtc gcgtggggat ttgccattgt caacttcgtc tggtggatcg
     4021 gtatcggcca cgcgggcacg ttgatatccg ccattctgct tttgctgaag caggaatggc
     4081 gcacgtcgat caaccggttt gccgaggcga tgacgctctt tgccgtggcc tgcgccggac
     4141 tgtttcccat cctgcacctt ggccgtccct ggctgttcta ttggcttctg ccttatccga
     4201 acacaatgga aatgtggccg cagccgcgca gtccgctgat ctgggatgtc ttcgcggtga
     4261 gtacgtatgc gacggtttcc ctcctgttct ggtttgtcgg tctgattccg gatctggcga
     4321 ctttgcggga ccgggcgcgt aaccgcgcaa gccggatcgt ctacggaatg ctggcgatgg
     4381 ggtggcgcgg ttccgcggta cactggcacc gctacgagac cgcgtatctg ctgctcgccg
     4441 gtttggccac acctcttgtc gtctcagtcc acacggttgt cagcttcgac ttttcgattg
     4501 cgcaactacc cggctggcat accaccattt ttccgcccta cttcgtagcc ggtgctatct
     4561 attccgggtt tgcaatggtc ctgaccctgg cgattcccat ccgcgcgttt tacggccttg
     4621 aggatttcat cacaagacgc catctcgaca acatggcgaa ggtgatgctc gggaccgggc
     4681 tgattgtcgc ctatggttac ctgatggaaa cgttcatggc atggtacagc ggtctcgagg
     4741 ccgagcggta catgatgtgg aaccgtatta caggaccgta ttggtggtcc tacgcgttgc
     4801 tgattatctg caacattgca gttccacagg cgctctggtc gagacgtatc cgggccaatg
     4861 ttttcctgtt gtggattctg tcgctcgtta tcaacgttgg catgtggctc gagcggtatg
     4921 tcattgtcat caccagcctg catcgggact ttctgccgtc ttcctggggc atgtatcacc
     4981 cgacgatatg ggactgggcc acgtttatcg gaacgatcgg gttattcctt tcgctattgt
     5041 tcctgtttat ccgcggcctg cccatgatct cgattttcga aatgcgcacc cttgtaccgc
     5101 aggcgcgaag aggaggcagc cactgatcca tggcgtacga acggctttac ggcttgctgg
     5161 ctgaattcga taatccggac gatctgttgc atgctgcgcg aacggtccgc gagaaaggct
     5221 accagtacct ggacgcgtac tcgcccatgc ccgtgcacgg tctttccgag gcggttgggt
     5281 tccggcatac ctggcttccg ctcgttgttc ttatcggggg attagcgggg gcggcgggcg
     5341 gcttttttct ccagtattgg atcaccgtcc tgcactatcc gttgaacatc ggagggcggc
     5401 cgttgaacag ttggccggcc tacatcccca tcacgttcga atgcggtgta ctggtcgcgg
     5461 cgattaccgc ggtactggcg atgattgctc tgaacggctt gccgcgcccg taccatccgc
     5521 tgttcaacgc gccgcgattc gcactggcga gccaccaccg gtttttcctc tgcatcgaga
     5581 agcgggatcc actgttcgat ccggcgaaaa cacgggatct gctggagagc ttccatactc
     5641 ggggggtttg tgaagtaccg gtttaggttg atggctatgg ttttgccgat catggtactg
     5701 gccggttgcc gccaggacat gcacgaccag cctcgctatg aagcgctcga aaggagtgag
     5761 ttctttcccg acggccgctc cgcgcggctc ccggtgcaag ggaccgttgc gcgcgggcat
     5821 ttgcaggaag acagggcgtt ctacacgggc aagcaagacg gaagactggt tcagaccatg
     5881 ccggtgaacg tcgaccggca gcttctcgcg cgtggccggg agcggttcaa tatctactgc
     5941 tcgccgtgtc acggtacctc cggcatgggc aacggtatga tcgtccaacg cggcttccgg
     6001 tctccgccag cgtttcatac agaccgcctt cgtcaggcac cagcgggtca ctacttcgac
     6061 gtgatcacca acggtttcgg tgcgatgtac agttatgctt cccgggtgga accgcgagac
     6121 cgttgggcca ttattgccta catccgggca cttcaagtca gccagaccgc tacgctggcc
     6181 gatgtgccgg agaaggaacg gacggaactg gaaaagcaga cggaagatca acaaggccag
     6241 ccgcaaggac caggggggca aaagttatga cgccggcgga accactgccg atcgatatcg
     6301 gacggtacag aacgtacgcg gccatcggcg gagttgccgg actggcggtt tgtgtcctcg
     6361 gctggctact tgatccgggg cagttctttc actcctacct gttcggattc atgttctggt
     6421 tcggcatcgg atcggggtgc ctggcggtct tgatgctcca tcacatggtg ggaggggaat
     6481 ggggcttcgt aattcgccga ttgctcgaaa cgggtacccg cacagtaccc ctcatggcgc
     6541 tgctgttcat cccgattctg tttggcattc cttatctcta cgagtggtcc catccggaag
     6601 ttgtcgcgaa cgatcacctg ctccaggaga aacaggcctg gctgaacgta ccgtttttca
     6661 tcgtgcgcgt ggtgatctac ttcgcgatct ggatcgggct tgcgtttttt ctgaaccgct
     6721 tctccagcca gcaggatgtc agtgaagatc ccgcgctgcc taccaggagg ctacggaata
     6781 tcagcgcacc tggaatcatt ttgtggggac ttgccgtgac cttcgcttcc atcgattggg
     6841 tgatgtcgat ggagccgcat tggttctcca caatgtacgg cgccttgtat gttgtcggcc
     6901 aggcgctgtc ggcgttcgcg tttacgatta cggtggtcat tctgctttcc acccgtccgc
     6961 cgctttcgcg cgtcctgggt gtgtcgcaac tgcacgacct cggaaatctg ttgtttgcgt
     7021 ttgtgatgct ctgggcctac gtgtccttct cccaattcct catcatctgg tccgggaaca
     7081 taacggagga gattccgtgg tatctgcggc ggaccagcgg gggatgggtg ttgctgccca
     7141 tcctgctgat cgcgtttcac ttcacgatcc cgttcttcgt gctgctgatg cgggatgtga
     7201 aaaggcgcgc gaaccggctg gtgtggatcg ccgcatggat cgtcctgatg cgcctggtgg
     7261 acttattctg gctgatcgag cccgcctatc atgggcgcgc gctgtctgtt cactggctgg
     7321 atctggccgc gcccattggc atcggtggat tgtggatcgc atggtttctc tggcagcttc
     7381 cggcgcggcc tctggtgccc ttgcaagctc cacagatggt aaacggggat acaccacatg
     7441 cccactgatc atacgggaac ggatcatttc gaggttcggc atgagacgaa ggacgtcaat
     7501 atccgggcca tcgtcatttt cggtatcgca ctggcggcgg tatgtgttct ggttctcgcg
     7561 ttccttgcgt ttctattcgg cttgttgggg aaggtgcaac ccgaacctgc gcccacgtcg
     7621 gccctggaag ttccgtttca gccgccgccg gagccaaggt tgcaggttag gccgtccgtg
     7681 gatatggaac gtttgaatgc ttttcaggac aacatcctga atcgatacgg ctgggtggac
     7741 cggtccgcgg gcgtggtccg gattcccatc gaccgcgcaa tggagattgt cgcggaacgg
     7801 ggcatcccca aaacattggg cgccggcggg ccggagttcg aagtccccgc accggtgccg
     7861 ccgggagtta agcgttgagg tggtggaagt gaagtatctg gtaattgcag tcaccttttg
     7921 cgcggttctg ttggctaata cctcatccaa tgaggtgccg cagcaactgc gtggaatcgg
     7981 catcgatcag aaattgaatc agcagattcc gctggatgcg atgtttaagg acgagtatgg
     8041 caggaacgtc cggctgggag actacttcgg aaaaaaaccg gtcgtgctgg ggctggttta
     8101 ttacgaatgt ccgatgttgt gcaatctcac gttgaatgga atgctgcgaa gtcttcgcgc
     8161 agtgaatctg aacatcggca aagagtacga tgtactcaac gtcagtttca atccgcgaga
     8221 ggggacggaa cttgcggcgg ctaagaagga agcctacagc aggcgttatg gacgcgaggg
     8281 tgtccggaac ggctggcatt ttctcaccgg caacgaagag aacattcgca agttagcgga
     8341 tgccgttggg ttccactacc ggttcgatga gaagagcggc cagtacgtgc acgccagcgg
     8401 gatcatgatt gtcacgcccg aaggcaggct ctcccactac ttctacggga tcgagtttcc
     8461 gccgcgcgat gtcaagttcg ggctgatgca ggcttcgaat aaccggatcg gctctcccgt
     8521 cgatcaactg ctgttgtatt gctaccatta cgaccccgaa acgggaaagt acggtctgat
     8581 catcatgaac acgattcgga tcgcaggtgg gttgaccgtc gcggggctcg tcctgatgtt
     8641 ggtagtgtta tctcgccgca accggcgcag gaaattggag aatgcgagaa cttttccggt
     8701 tcttccctga tagtgcatcg tcgatatccg atgcggtgga cgcgctctat ttgtggctga
     8761 ccaccgtcag cacaattatg acgctgctga tctttctggc catcgggtac ttcgccatcc
     8821 gctaccggcg gcgggagccg ggggaagttc ccactccggt tctcggttcg ttgcggctcg
     8881 agatcctgtg gtctgtcatc ccgttcctgg tcatgctgtc gttcttcaca tggggtacgt
     8941 atctgtattt tcacagccag accagtccga aaggcggtat ggaggtattc atcaccggta
     9001 aacaatggat gtggaaaatt cagcatccat cggggccgcg cgaaatcaac gaactgcatg
     9061 tgccgatcgg ggtacctgtg aatctgacac tggcgagcga ggacgtgatt cacagcttct
     9121 ttgttccggc attccggatc aagaaagatg tggttccggg ccgcctgaac cgtgtgtggt
     9181 tccaggccac caagcccggc aaatatcatc tgttctgcgc ggagtattgc gggacggagc
     9241 attccggcat gattggctgg gtctacgcca tggagaagca tgaatacgaa cagtggctca
     9301 gtggcggagg aggtgaaggc tccatggcga cgcgaggaga aaagctcttc cagcaatatg
     9361 gctgtgcgag ctgccatacc atggagaccg ccggacgctg tccttcggtg cggggccttt
     9421 acggcaggca ggtattacta agaggcggcg agcaggttac tgccgatgat tcctacctgc
     9481 gggaatccat tttgaatccc cgtgcaaaga tcgtcgccgg ctttgagccg atcatgccca
     9541 cct
//
LOCUS       DASTMK010000029         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_449799, whole genome shotgun sequence.
ACCESSION   DASTMK010000029 DASTMK010000000
VERSION     DASTMK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449799"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFQ79_01000"
     CDS             complement(<1..266)
                     /locus_tag="VFQ79_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138251.1"
                     /translation="MSRLRALFLAPVWVIVGSLLFAPLAIVFLYSLLTRGAYGGLAPP
                     WTFENYQRLADPLYLGIAAQSFLLAGIATLLCLLIGYPLALFIS"
     gene            complement(263..1237)
                     /locus_tag="VFQ79_01005"
     CDS             complement(263..1237)
                     /locus_tag="VFQ79_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0138252.1"
                     /translation="MDAVLELRNVSKHYSSHRVVNGVSMEAPRGAFYSLLGPSGCGKT
                     TTLRIIAGFEEPTAGEILLNGNSLLGLKPYHRNVSTVFQNYALFPHLTVRQNIEFGLR
                     YQPERRRNGSNRQTAGEVIEMMQLGGKETRKPAELSGGEKQRVALARSLVLQPDVLLL
                     DEPLSALDPRLRKQLRAELKSLQRRAGITFLFITHDQEEALSLSDRISVMHAGRIEQT
                     GRPEEIYLKPATRFVASFVGHVNWINGIGIRPEATRIGREAPATGARIVPATAVNCVF
                     LGNCVQVEARLLSGAPVLVELPFGHERPTPGETTHVWWRPEDELRFRE"
     gene            1330..1800
                     /gene="greA"
                     /locus_tag="VFQ79_01010"
     CDS             1330..1800
                     /gene="greA"
                     /locus_tag="VFQ79_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU0138253.1"
                     /translation="MEDLKKKLQDEITSLEYELRNELPKEILRARAHGDLSENAEYHA
                     AKERQRFVDARLAQLKKRLADFSMVDLARIPRNKVGLGSTVVVMDVDKEEKFTYRLVT
                     SEESDVAHGLISTSSPIGRGLVGKEVGDTVKIQIPGGWREMEILRLTTIHDEES"
     gene            1827..2456
                     /locus_tag="VFQ79_01015"
     CDS             1827..2456
                     /locus_tag="VFQ79_01015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0138254.1"
                     /translation="MTYTRAIGVACNKVILLIVRGLALAKIHPNVLTFIGLLINVAAA
                     VLLASGRFMTAGFVITGAAIFDMVDGRVARETNTVTRFGGFFDSVLDRYSDLALLMGL
                     LVYYASIDRFFYIILTAIVMTGSVMVSYTRARAENVIPLCKVGFMERPERIVLLIIGA
                     LFDRMAPVLWVIAVLSNLTVIHRMGHTWQETKRLEEAQLRPAAGISHRK"
     gene            2594..2854
                     /locus_tag="VFQ79_01020"
     CDS             2594..2854
                     /locus_tag="VFQ79_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU0138255.1"
                     /translation="MAYVIAEPCIGTKDTACVDACPVDCIHPKKDEGDYETAELLYID
                     PVECIDCGACVPVCPVSAIFALDDLPEKWANFAQINADYYKR"
BASE COUNT          606 a          842 c          876 g          559 t
ORIGIN      
        1 ctcgaaatga acagggccag cggatagccg atcagcaggc acaacagcgt ggcaataccg
       61 gccagcagaa acgactgcgc cgcaatgccg agatagaggg gatcggccag ccgttggtag
      121 ttttcgaaag tccagggcgg cgccaagccg ccgtacgccc ccctggtaag cagactgtag
      181 agaaagacaa tcgccagcgg cgcgaagagc agcgacccga caatcaccca aacgggcgcg
      241 agaaacaggg cgcggagacg actcattccc ggaaccgaag ctcgtcttcg ggacgccacc
      301 agacgtgcgt ggtctcgccc ggtgtgggcc tctcgtgccc gaagggcagt tcgacaagaa
      361 cgggcgctcc ggaaagcagg cgggcctcca cctgaacgca gttgcccaga aagacgcagt
      421 tcaccgccgt tgccggcacg attctcgctc ccgttgcggg cgcctcgcgt ccgatccgtg
      481 ttgcctcggg acgaataccg attccattaa tccaattgac atgcccgacg aaggaagcca
      541 cgaatcgcgt agccggtttc aggtagatct cttccggcct gccggtctgt tcgatgcgtc
      601 ccgcgtgcat gacggagata cggtcggaaa gcgacagcgc ctcttcctgg tcgtgcgtaa
      661 tgaaaaggaa ggtgatgccg gcgcgtcgct gcagggactt caactccgcg cggagctgct
      721 tccgtagccg gggatcgagg gcggagaggg gttcatccag gagtaacacg tcgggctgca
      781 atacaagaga ccgcgccagg gcgacccgct gtttctcccc gccggatagc tcggcgggtt
      841 ttcgcgtttc ctttcccccg agttgcatca tctcgatcac ttcgccggcg gtctggcggt
      901 tcgatccgtt cctgcgccgt tccggctggt agcgcaagcc aaactcgatg ttctggcgca
      961 cggtgaggtg gggaaacagc gcgtaattct gaaatacggt gctgacgtta cggtgatacg
     1021 gcttcaaccc caacagcgag tttccgttca ggaggatctc tccggccgtg ggctcctcaa
     1081 aacccgcgat aatacgcagc gtcgtggttt tgccgcatcc ggaaggaccc agaagggaat
     1141 aaaaggcgcc gcgcggcgcc tccatcgaga cgccattcac cacacgatga gacgaatagt
     1201 gcttcgagac attccgaagc tccagaacag catccatatt gaaatgaatc gtatcgcaaa
     1261 ccggtcgagg cagcgacagg ctgcctcgac ggctgaacta taataaggct ctgggagccg
     1321 ttttcctgaa tggaagatct caagaaaaaa ctccaggacg aaatcacgtc gctcgagtac
     1381 gagttgcgca acgagttgcc gaaggagatt ctccgggcca gggctcacgg cgacttaagc
     1441 gaaaacgccg agtaccacgc cgccaaagag aggcagcgat ttgttgacgc ccgccttgca
     1501 cagctcaaga aacgtctggc cgacttctcc atggtggacc tcgccagaat tccccggaat
     1561 aaagtgggat tggggtctac ggtggtagtg atggatgtcg acaaagagga aaagtttacg
     1621 tatcgcctgg ttaccagtga ggaatcggac gtcgcgcacg gcctcatctc gaccagctca
     1681 cccataggcc ggggcctggt gggcaaagag gtcggcgata cggtcaagat ccagatcccg
     1741 gggggttggc gggagatgga aattctccgg ctgaccacca tccatgacga agagagctaa
     1801 gatagacgag tggaaaggcg aggtctatga cgtacactcg tgcgatcgga gtcgcgtgca
     1861 ataaggtcat tctcctgatc gtacgcgggc tggcgctggc gaagatccac cctaacgtcc
     1921 ttactttcat cgggctgctg attaacgtcg ccgccgcggt gctgctggct tccgggcggt
     1981 tcatgacggc cggattcgtc atcaccggag cggccatttt cgatatggtg gatggccgcg
     2041 ttgcgcgcga aacgaacacg gttacccgct tcggcggctt tttcgattcc gtgctcgacc
     2101 gctactccga tcttgcgctg ctgatgggcc tgctggtcta ttacgcctcc atcgaccgct
     2161 ttttctacat catcctcacc gcgattgtga tgacgggctc ggtcatggtc agctacactc
     2221 gggcgcgcgc ggaaaacgtc ataccgctgt gcaaggtcgg attcatggaa cgcccggagc
     2281 gcatcgtcct cctgatcatc ggcgccctgt tcgaccgcat ggctcccgtg ctttgggtga
     2341 ttgccgtact atcgaacctg acggtgatcc atcgcatggg tcatacctgg caggagacga
     2401 agcgcctgga ggaggcgcag ttgcgccccg ccgccggcat ctcccaccgc aagtaggtct
     2461 ccgctcggcg tccgtctttt cccgtccaac ccgcccggga ggtgcccgaa cgtcttccac
     2521 tttgtccaac ctgcccggat gtgctataga tgttgtttct gttggactcg gagggagaag
     2581 agggagtaac gttatggcct atgtcatcgc agaaccctgc attgggacca aggatacggc
     2641 atgtgtagac gcatgcccgg tcgattgcat ccatcccaag aaagacgaag gggattacga
     2701 gacggccgag ctgctgtaca tcgatccggt ggaatgcatc gactgcggcg cgtgcgtacc
     2761 ggtctgcccg gtgtccgcta ttttcgcgct ggacgatctg ccggaaaagt gggcaaattt
     2821 cgctcagatc aacgccgatt attacaagcg ctgaagcccg tcgccggcta gggcctgtaa
     2881 gtc
//
LOCUS       DASTMK010000030        25517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_481836, whole genome shotgun sequence.
ACCESSION   DASTMK010000030 DASTMK010000000
VERSION     DASTMK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 25517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25517
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481836"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFQ79_01025"
     CDS             complement(<1..668)
                     /locus_tag="VFQ79_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138256.1"
                     /translation="MRRAALLLLFLSITFPSYGQSGSYDILITGGRIVDGTGNPWFQG
                     SLAIDGNRIIAIGRTPPGSPRRTIDATGKVVAPGFIDIHTHSRGGIFQVPDAGNYIRQ
                     GVTTVMEGNDGGSPLPLPEFFEKLRKTPVAVNLGLFVGQGSVRQKIVGLENRKATPAE
                     IREMEDLVRTAMLDGAFGISTGLFYVPGNYTPTEEVIALARIAGEMGGMYISHMRDEA
                     AGVL"
     gene            913..2205
                     /gene="larA"
                     /locus_tag="VFQ79_01030"
     CDS             913..2205
                     /gene="larA"
                     /locus_tag="VFQ79_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEU0138257.1"
                     /translation="MRVNVAFGKTGLSIDLPDGFEYRLLEARTATPHKDPPAAIDQAL
                     DTPIGSLPLRQLALGKKSAAISVCDITRPAPNRIVLPKVLERLEAGGIPSGDIVILIA
                     TGLHRPATPAEIREIVGADIASRYRVENHNARELSEHQHLGTTASGTPVYIDRRFASA
                     DLRITLGFIEPHLMLGFSGGRKLVAPGLAAQETIKVLHSPKFMREPRAVEGSIEDNPL
                     HRELLEIARMARHDFIVDAALARDRQIAAMFAGDPVAAHRKGVEFVSSVMLEELDRPV
                     DAVITTSAGYPLDLTFYQAIKGITAASHILKPGGRILLMSACDEGAGAPEFCRMLKEP
                     HSYREFLQQILGREVVVDQWQLEKLALVNLNAGILFYVPGLPQEYHSSLWGRAFSSAP
                     AALEALFEGLEPDAAIAVIPEGPYVLAKIRSPREAQAI"
     gene            2216..4267
                     /locus_tag="VFQ79_01035"
     CDS             2216..4267
                     /locus_tag="VFQ79_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0138258.1"
                     /translation="MPEVCHSVCALDCPDCCSVLVTVESGRATRLRGNPDHPVTRGFL
                     CGKVARYLDREYSPERLLYPLRRAGAKGEGRFERISWDEALATIAGELRSISERFGPE
                     AILPYSYAGTMGYLNGSGMDRRFFHRLGASRLDRTICSAAGGAGLAQTLGIRYGTEPE
                     QFRESKLILAWGANIHGTNVHLWPFIVEARRKGARLYVIDPIRTRTAELADRHFAIYP
                     GSDLALAFGMAHVIIAEDLYDHDYVERYTSGFESLARRASEYTPERVASLTGLAADDI
                     RQLAREYATTRPAAIRLNYGVQRSERGGGAVRAIATLPALAGSWREPGGGLQLTTSGA
                     FQIDRDALEMPGLQEASPLGREARVVNMARLGEALTVLDNPPVKALVVYNSNPAAIAP
                     NQNLVLRGLRREDLFTVVLEQFQTDTADYADIVLPVTTFLEHTDLYFAYGHYYLQLAR
                     PALEPPGETKSNFEIFRLLAERLGFEEQAFRETEDDVIRAVLAGSKGAVRGITLEQLE
                     TEHSVRLRLAGDGDAFLPFANGGFGSEDGKCYLSMEDLDYVPPDESRLGEESIRLRYP
                     LELVSSKNDDSMNSTFGHRGFVDEQTSVLWMHPGDASERGIEPGEQVRVFNNRGSCIL
                     KARITDGVRTGVVRAPSVRWVKRSPGGKGINVLTSDRLTDIGGGPVFYSCLVQVERCG
                     D"
     gene            4255..4794
                     /locus_tag="VFQ79_01040"
     CDS             4255..4794
                     /locus_tag="VFQ79_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138259.1"
                     /translation="MRRLDRKAIWIATAALVVVSAGCSRQEKVRVEMTEEEGAQLATM
                     VHVADPRANIQLLKGFHDIEQNAWRWTMSKFSVALRPPVGAAQAGATLQLKFSIPDPV
                     LKHTKDITLSASVNGSPLPPETFKTTGQHVYSKDVPPAALASDAITVDFALDKFLKPG
                     DVDTRELGVVVTSVGLEPK"
     gene            4791..6737
                     /locus_tag="VFQ79_01045"
     CDS             4791..6737
                     /locus_tag="VFQ79_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138260.1"
                     /translation="MRAGEEPLLHNPATETHSNASAFGRALSRVKIGTVFYWTVPPLL
                     CLLLFHRSLNGWFMQDDFAWLGLYGSVNSWEDLQRVLFTPYAQGTLRLWSERAFFLVF
                     YALFGLNAFPYHLWVFLTQVANLALLTHITFRLTGSRAAGFLAPLFWITNAGIIVPMS
                     WACAYNQILCAFFLLLAFHFWLKHIDTGLWRYYWWQGAAFLMGFGALEINVVYPAIAA
                     AYALLCARRYFVRTLPLFAASGAYTVFHTLVAPKDKSEIYAIVIDSKIFNTLLTYWNW
                     GVGPYRLAGITEELPQWFAPATVAVLTAALLGLVVWSAFERRFLPAFFLSWFGIVILP
                     VLPLRNHISDYYLALPSIGIAMLMAYGLVMAWRYGPRAMAVAIPVAVLYVASGVPVID
                     AQTRWYEKRGQAVKNLLTGVARARELHPRKLILLTGVSSELFWQGVYDRPLRLLDIDN
                     VYLAPGTEKSIATYPEMAPLSNYVLPASITLRALKRETAVVYEVHDGRLRNITKPYTY
                     RALMEFAKEEPRQVVAGNEFYSGQLLEGWYSIENGYRWISQKASLRMGGPTQDGQRLY
                     VTGECPEALLQAGPLGFSILVDGSPVYAGTVRKAGEFDVVAKLPLTAVGKDSVIITVE
                     VSRTLSPAGESRKLGLVFGTFEIR"
     gene            7102..7527
                     /locus_tag="VFQ79_01050"
     CDS             7102..7527
                     /locus_tag="VFQ79_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU0138261.1"
                     /translation="MRRIRVQRIEEGEVMTKTELNKFKQILEARLAELALVLKNREGI
                     AIEKSPDALDEVQNAAERELAIRNLDRESNLLRNVRAALRRIAEGHFGVCIHCEEEIS
                     PKRLNAVPWAPYCIQCQELADRQQEGDGSDTEEMLVNAA"
     gene            complement(7597..8265)
                     /gene="pgeF"
                     /locus_tag="VFQ79_01055"
     CDS             complement(7597..8265)
                     /gene="pgeF"
                     /locus_tag="VFQ79_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEU0138262.1"
                     /translation="MSAVHKTSAGIYRIEALEWPWLDHGFGTRASNFWPPPSKLAMVK
                     QVHSDRILGAGASAGYIGEGDALVSNTAGIYVGVRTADCQPILMVDPEVRAVAAIHAG
                     WRGTAACLAAKAVDRLRRDFGAEPVRLVVAIGPGIGVCCYEVGPEVAIQFKELFPERG
                     KELERKTHLDLMEATRRQIAAAGVPDGNIHIVPLCTFCSDEFHSYRRDKDKGRMISAI
                     GILE"
     gene            complement(8269..8829)
                     /locus_tag="VFQ79_01060"
     CDS             complement(8269..8829)
                     /locus_tag="VFQ79_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU0138263.1"
                     /translation="MSYIQNQFEKNFLTTSVDYVFNWARKSSVWPLTFGLACCAIEMI
                     ASSASRFDIARFGAEVFRPSPRQSDLMIVAGTVTLKMAPVLKRIYDQMPDPKWVISMG
                     ACSSVGGPFNTYAVLQGVDKIVPVDVYVTGCPPRPENLFYGLMKLQDKIDQMTLARRP
                     TEVRLDESMLEDFKKQVMVAQIQTPA"
     gene            8906..9850
                     /locus_tag="VFQ79_01065"
     CDS             8906..9850
                     /locus_tag="VFQ79_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0138264.1"
                     /translation="MQKRALGRSGISVAPLVFGGNVFGWTADRPTSFALLDRFAEQGF
                     NAIDTADVYSAWAPGNSGGESETILGEWLRRRGRRDDLVIMTKVGSWQKHKGLKVSTI
                     TAAVEDSLRRLQTDYIDVYFAHIDDTSVPLAETMGAFSRLIQAGKIRAAGASNYKVQR
                     LSEALDTAASNGLARYEVLQPNYNLYDRSDFESGLAKAASDNEVGVVCYFGLAAGFLT
                     GKYRSEEDVKGKSRAGMVKGYLNERGYRILKALDEISAGLSATQAQVALAWLMARPGV
                     TAPIASATSLHQLDDALGAARLDLSSEAIAKLDEASAY"
     gene            complement(9844..10140)
                     /locus_tag="VFQ79_01070"
     CDS             complement(9844..10140)
                     /locus_tag="VFQ79_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138265.1"
                     /translation="MIVRIRLKPGAKVTKKPGRKQKVASALASLLTPASLMACVLSLW
                     RLAADLQWTETFAISEGLFSHWQVWMLIAVALQSGAVLLNRYARASQQTGERVQ"
     gene            complement(10147..11496)
                     /locus_tag="VFQ79_01075"
     CDS             complement(10147..11496)
                     /locus_tag="VFQ79_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEU0138266.1"
                     /translation="MSEITKFLLRDNQIPSSWINVLPLLPKPMEPPLHPGTRQPLSPD
                     DLRPIFPDSLIEQEFSPKREIDIPGEVLDIYRLWRPTPLFRAYRLERALDTPAHIYYK
                     YEGTSPAGSHKPNTAIAQAWYNKQAGIRRLATETGAGQWGSALALACNLFGMECTVYM
                     VKVSYQQKPYRRIMMNAWGAQVFASPSEKTQSGRAVLAEFPDSPGSLGIAISEAVEDA
                     AGSSDTNYSLGSVLNHVMLHQTVIGQEVQEQMRLAGEEPDVVIGCFGGGSNFGGIALP
                     YIEQKLKGAGPRLLAVEPAACPTLTKGVFAYDFGDVAKLTPLIKMFTLGHDFVPPGIH
                     AGGLRYHGASPLASHLLELGAIEAQSYRQLPVFEAALQFARIEGILPAPESAHAIRAA
                     MDEAIAAREEGKARNILFCLSGHGNFDLASYENFMEGKLHDYELPAQEISRAIAALP"
     gene            11550..13106
                     /locus_tag="VFQ79_01080"
     CDS             11550..13106
                     /locus_tag="VFQ79_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU0138267.1"
                     /translation="MDRRFFLNLAGSAAVSNLLRPAAFSANAPRPNILLIYADDLGYG
                     DLGCYGAKNVTTPNLDRMAKAGVRFTDAHSSSATCTPSRYSMLTGEYAWRKKGTGVLP
                     GDARLIIDPKRPTLPNMLQNAGYRTGVVGKWHLGLGDGNLDWNGEIKPGPLEIGFDSA
                     FLMPATGDRVPCVYVKDRRVVGLDPKDPIRVDYQKPIGDEPTGRKNPELLKMHPSHGH
                     DMAIVNGVSRIGYMTGGKSALWVDEDMAAEFVKRGIEFIDQKRREPFFLYFATHGIHV
                     PRLPNRRFTGKTSMGPRGDAIAELDWTAGQLLDHLERRGLAKNTLVIFTSDNGPVVDD
                     GYKDDAVEKLGSHKPAGHLRGGKYSNFEGGTRVPMIARWPERIKSGVSGALVGQQDFF
                     ASFATLSGAKLGEDAAPDSFDVLPALLGESRTGRDWIVEHARALSIRRGEYKLIETNP
                     GLAVSKNTNIEMGTGSGPQLYRVTQDPGEQKNLAGAMPDKVKELSALLEKVKASPRSR
                     VDVRTPVDNN"
     gene            13174..13899
                     /locus_tag="VFQ79_01085"
     CDS             13174..13899
                     /locus_tag="VFQ79_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0138268.1"
                     /translation="MFRMVKLFPLLFMFGRLQAAVIDTNDPGTIAAFQSGATVVNFES
                     VTGLTPQAITAYTAGDAVSAGSFLFDQISGVQFSVGGAPGTNMPALYQLSGAIQGDAT
                     SPSTVLGPVDFDFTTKFSSGALIEIFFPVKVSKVGFWLNPSLGNVNIIAADTNFAFSQ
                     ETETTLETGNVTAGNFVGIVRPTADIGGFKIIGLGATGFTIDDFTYGGASVSPVPEPA
                     SLMLAGAGLLVAAFLRWRRHYAR"
     gene            14046..15494
                     /locus_tag="VFQ79_01090"
     CDS             14046..15494
                     /locus_tag="VFQ79_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138269.1"
                     /translation="MSVQVFLQGRVFGIEQFLSSSLHVAGGEKTDERLLIGRSRWVAL
                     LAEVLPRALLAELGLSRMLLGTSGGGQFLLVIPDEARPEAERFLRAAAADISRLSNRM
                     VTLDWAATENLGDWTLVRKRLNEEMRKRRSTPAADGGADFFQPFPLEPSSTDADEYFT
                     LEMAVAIREAQLVGWSPETPGQVLADKGKHTWAIGASADAIPLARHSAPDDTGARSAD
                     RNELAGRAEGRAVWGVLRGDVDNFDIRIRRLTTIEEHVQLSVMYKQFFAGELEIVCSM
                     PEFWRKVSIIYSGGDDFAVYGSWDGLIQLAREIQRLFHRLTEENMKDFPGLEGKTISM
                     AMALAPEPDAPLAAVYQESGRKLALAKATEKHCIHLLGRTLEWKQLAYASELKDNMSR
                     MIREFAAPPQFLRELAGLYRETADGRDGALERPWRLHGRLNRVLGPTRDRDFQRSRTT
                     LLTDLVGKGASQKRLRPSGRVALEWAKLSTGV"
     gene            15494..15964
                     /gene="csm2"
                     /locus_tag="VFQ79_01095"
     CDS             15494..15964
                     /gene="csm2"
                     /locus_tag="VFQ79_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HEU0138270.1"
                     /translation="MMAEAGQEQREQRRSRPPREGREGGPDRGRWEDRRGDRGRPEGP
                     PEIDLDKLIGDSLYLDNQAHSVVSRMRDMDSGTKSQLRRFYNAVRRACRAPENERQHQ
                     FVMLRARLAYTIARHSLRSLDTLEKLLLQVTRKNDARGYERFRDLFEAIVAYNE"
     gene            16008..16841
                     /gene="csm3"
                     /locus_tag="VFQ79_01100"
     CDS             16008..16841
                     /gene="csm3"
                     /locus_tag="VFQ79_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HEU0138271.1"
                     /translation="MSSHTAQTQLTFIGKLIIEGELHCDTGLHIGAGKGQLEIGGADN
                     PVVKDAFGRPYIPGSSLRGRLRSLLEQSSGMAIPSELVYLSKRRGQEVRIHQSDRPDD
                     VICLLFGRNPGRIERVSGEALESSVATPARLTVYDAPLVPESITVQMRENLDDELTEV
                     KSENAIDRITSQANPRTLERVPAGARFHIRMVLDILCEEDRELLPRLAEGLRLLEDDT
                     LGGGGSRGSGRVHFSGLKMVWRGKEFYSSGAAETELLAGADVSGLQSLANESGLAEKL
                     A"
     gene            16847..18547
                     /locus_tag="VFQ79_01105"
     CDS             16847..18547
                     /locus_tag="VFQ79_01105"
                     /inference="COORDINATES: protein motif:HMM:NF037153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138272.1"
                     /translation="MLPALLVRLRPRGPWRFGPDDGARNRVDTIGHSDTFYCALSHAM
                     RMTGMLEEWLEATTSTGGSPAVRFSSLYPFQGETLFFVPPQTLWPPPPSTRIRWKSAR
                     FVPAQVLSPLLSEKPLDEGRWTIDAHSECLVSSGSTGPFRVSLRGAAAVDRLDNGKTA
                     PHATACLEFTENSGMWTVFVFRDGEARERWLPAVQSALRLLADSGLGGERSRGWGRSD
                     PPEFREGEFPSIIARIPAVDRTEDQEEAAQNFETGHWLLSLFSPAEQDTIDWDRGSYQ
                     LVVRTGRTGGAHSGELTRSVRMIGEGSVLAGAAKPAGTVRDVRPEGAGEAALRWGVAL
                     SIPIRVRRAPAALPVPPVAPSGQPEPQQVEPTPEAELAPQQVEPAPETEVEPQQSEPA
                     EEPETAESGVLSLLSPPPGEAMPPVETQQEPTPEAEAGEPESVAVEPVPEILPEEPLP
                     PAEEAQSQEPEPEEPEKSGVLALLSPPPGEAMPPVETQAEPEPMPETRPEEPLPPAEE
                     AQSQEPEPEEPEKSGILALLSPPPGEAMPPVETQEEPAPPPETSDETEPPPSGPEEHT
                     "
     gene            18544..19695
                     /gene="csm5"
                     /locus_tag="VFQ79_01110"
     CDS             18544..19695
                     /gene="csm5"
                     /locus_tag="VFQ79_01110"
                     /inference="COORDINATES: protein motif:HMM:NF015728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HEU0138273.1"
                     /translation="MKYRLTCLTPLLVGDGRRLSPIDYMVWKDQVNVLDQNRIFNLLA
                     KGPRLEGYLMQLKKADKLDFASWGGFAQNFADRRIPFEHKSMTAYWERARAENLFIPT
                     FAAGLQGPYIPGSALKGALRTGLVFSRWSGNTLKDIAGRMQGERSPRRPGDAAEEQAI
                     GAPEYSRMKAVAISDSVPVSYASMKVYLLRVATLVSRGAGRYELGWKQAPRGSVDGRR
                     PEDSTPWFAEMAAPGTTFEGGLHLNQFFHQPEIVRALRWREPPDQSTLFRAANRYAAD
                     LLARTKSYAAMAGLGRVEASVGELETEVSKAEAEGACILPLGWGAGLMAKSAWLDTGD
                     ETYRQVMSQVPLYAKAVQTGMPFPKTRRVVFFENQPAAMPGWVRLELMG"
     gene            19763..20659
                     /locus_tag="VFQ79_01115"
     CDS             19763..20659
                     /locus_tag="VFQ79_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0138274.1"
                     /translation="MYNAFFGFKENPFSLSPDPAFLYRSTQHEEALANLIYGVHSRKG
                     FIVLTGEVGTGKTTMLECLRDFLEAQQIEFAFIFNSRINADQFLEMIAYDLDLRCNRT
                     SKTEVLFALNQFLVQQAHHGRTTALIVDEAHNLEWDVLEEIRLLGNLESPKTGKLIQT
                     ILAGQPELERKLDAPNLRQLKQRIILRYDLKPFREADTVEYISSRMAKAGMPEQNVFP
                     VELLSEIHVRSQGIPRLINAICDNALLTCFALETRVATLEILDEVCADMRLEWPGGNR
                     QRGERPRRSGESYDPSAYQSRR"
     gene            20717..21592
                     /locus_tag="VFQ79_01120"
     CDS             20717..21592
                     /locus_tag="VFQ79_01120"
                     /inference="COORDINATES: protein motif:HMM:NF040125.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6528 family protein"
                     /protein_id="HEU0138275.1"
                     /translation="MRSMALPLLLAVSFAAAAEPATHDLLVCGWDEVYILNTATGRKS
                     FSWKAAERPELPASYKDKFRTTDECKLVEDGRILITASSDGVALADRRTGRTTFWGLC
                     ANAHSADLLPGERIAVACSVREAGGNRLAVFDARTPEKELFSTELYSGHGAVWDETRK
                     LLWALGHGELRAYALSDWETPRPSLKLENSYKLPSGGGHELSPDGDSHLVISGSRDVW
                     LFDRDNREFKPHPQLAGMHGIKSASVEPGSGRVAFTQSEPPNWWTSKIQFRNPDGVLT
                     REGERLYKVRWVKRD"
     gene            22236..22910
                     /locus_tag="VFQ79_01125"
     CDS             22236..22910
                     /locus_tag="VFQ79_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138276.1"
                     /translation="MNPLWNLAFLAAPVLQVLLISRVIAGACKSFPLVGVYAVFTFLA
                     TIAEGAAFLGVYPMSVLYERIYWVNEAILQILIFSIIISLIYQAMPATPRRNAASRWL
                     VASAVAALVLSLIGSYDVNLNAWMTQVSRNLAFASAVLSFLLWTLLLSRHSRNTQLLL
                     VSAAFGIQATGKALGHSLRYLKFVSFGDVVMILTYLVSLFVLWKALGISRASEERAVR
                     SGHTVV"
     gene            22971..23558
                     /locus_tag="VFQ79_01130"
     CDS             22971..23558
                     /locus_tag="VFQ79_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEU0138277.1"
                     /translation="MLLAREFIAYLSRQIVTKLTPQILEVASAESAAAMIGDIITEEL
                     AVEDKLNDEVREILSEYSEYMRREGVSYQEMFRRIKNTLITQRKVIRASGRDTGDQMK
                     LSRDKINDLSHKLVTAIRKSREFRLKRDPNEVRLEIVRAISELLTVEEKVDRVARQKI
                     RSQKREIPEGTEEWDLLHKRYYAEELKKFGIELSR"
     gene            23555..24484
                     /gene="rbsK"
                     /locus_tag="VFQ79_01135"
     CDS             23555..24484
                     /gene="rbsK"
                     /locus_tag="VFQ79_01135"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEU0138278.1"
                     /translation="MKPTVCVVGSLNMDFVVKVDHLPAPGETVLGRDFQMFPGGKGAN
                     QACAAGKLGSPSVDVRMVGRVGYDAFADHLKASLSAAGVDVSYVHATRSQPTGIALIW
                     VESAGQNSIVVAAGANNELAASEVEAMRAAMRDARYVLFQLETPLDTVEAALKLAREE
                     GAKTILDPAPAQPLSLDLLQLVDILTPNETEACILLGHAVGRVELNDVPVLANQLRSM
                     GPRSVVLKLGEKGCYYDDGVKQIHSPGFPVKACDTTAAGDTFNGALAIALAEGQDIET
                     ALRFANCAAAISITRTGAQASIPSRMEVDRFSG"
     gene            complement(24485..>25517)
                     /locus_tag="VFQ79_01140"
     CDS             complement(24485..>25517)
                     /locus_tag="VFQ79_01140"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HEU0138279.1"
                     /translation="AVLLIFVLSRLQPGVTEPQVFLGRIDPELEALQRMRASYNWVSL
                     WIGQWYGQYLFFTAAIAAAYFRLRSVMAPALKPFVLGLPLLGIVSVPLSYLLLEKMKW
                     ILIPQYQPARALLFVTLFAVILSSAAAIHAAQKGIWWESFLWFAAAYAVPLNTRVLDL
                     LWPDFSNVTLLRRFTVMIVLAAIGVLAAWADARKRKWASAVLLLLCCAPFWAMPGYAR
                     VVNFPALHTEPLQQLSQWARTETAKDSIFLFADAARDLAPGIFRARAIRPVYVCWKAG
                     GQVNYHKNVGEEWWERWRQTMASKFDPARVPEFAGLGIEYLVLKPEHRLPDRRPVFEN
                     EQFLVYRIR"
BASE COUNT         5234 a         7542 c         7714 g         5027 t
ORIGIN      
        1 tccagaactc ccgcggcttc gtcgcgcatg tgggagatgt acattccgcc catctccccg
       61 gcaatgcgcg ccagcgctat cacttcctcc gtgggcgtat agtttccggg gacgtagaac
      121 agacccgtag agattccgaa cgcgccgtcg agcatcgcgg tgcgaaccag atcttccatt
      181 tcccgtatct cagcgggcgt ggctttcctg ttttcaagac caactatctt ctggcggacg
      241 ctgccctgcc ctacaaaaag gcccagattg accgcaaccg gcgtcttccg caatttctcg
      301 aaaaactccg ggagaggcag cggtgagcca ccatcgttac cttccatcac cgtggtaacg
      361 ccctggcgaa tatagttccc ggcatctgga acctggaaaa tgccgccgcg cgaatgcgtg
      421 tggatgtcga taaaacccgg cgcaaccact tttcccgtgg cgtcgatcgt tctccgcggg
      481 ctgccgggcg gtgtgcgtcc aatcgcgata atacggttgc cgtcgatggc gagactgccc
      541 tgaaaccatg gatttccggt accgtcgacg atccggccgc cggtaatgag aatgtcatac
      601 gagccactct gcccgtagct cggaaatgtt atggagagaa agagaagcag gagggctgcc
      661 cggcgcatga gaattttatt ctacacgccg ggcgaccgtg atttttttac tcgtggtgtg
      721 tcaattcgcc actggcttcc tcgtgcttcg gcgaggaaga caacatggag aaaaacaatc
      781 caaggataag cagtgccgcg actaagcctg ttaccaacat gacaactccc ccaaagcctc
      841 atctatatga atgcaactta ggtgccaatg gtttcactcg caccacacaa tttttgtgct
      901 acgttctcga ctatgcgcgt gaacgtagca ttcggtaaaa ccggactctc catcgatcta
      961 cccgatggct tcgaataccg gcttctcgaa gccagaacgg cgacgcccca caaggacccg
     1021 ccagccgcca tcgatcaggc actcgacacg cccatcggca gtttgcccct ccggcaactg
     1081 gctctaggta agaaaagcgc cgcgatctcg gtatgtgaca ttacacgccc cgcgcctaac
     1141 cggatcgttc tcccgaaagt gctggaacgg ctcgaagccg gtggaatccc cagcggcgat
     1201 atcgttatcc tgatcgcgac gggtttgcat cgcccggcca ctccggcgga aatacgggaa
     1261 atcgtgggag cggacattgc ctcccgatat cgcgtggaga atcacaacgc gcgggagttg
     1321 tccgaacacc agcatctggg cactaccgcg agcggtactc cggtctacat cgaccgccgc
     1381 ttcgcctcgg cggacctgcg catcacgctg ggtttcatcg agccccacct catgctcggc
     1441 ttttccggtg gccgcaagct ggtcgccccg ggactggcgg cccaggaaac aatcaaggtc
     1501 cttcatagcc cgaagttcat gcgggaaccg cgggccgtgg agggctcgat tgaagacaac
     1561 ccgctgcacc gcgagttgct cgagatcgcc cgaatggcgc ggcatgactt catcgtggac
     1621 gctgcgctcg ctcgcgaccg gcagatcgcg gcgatgttcg ccggcgaccc cgtggcggcc
     1681 catcgcaagg gtgtggaatt tgtttcgagc gtaatgctcg aggaactcga ccggccggtg
     1741 gacgccgtca tcaccacctc ggccggatat ccgctcgacc tgaccttcta ccaggcgatc
     1801 aaagggatca cggccgcttc gcacattttg aaacccggcg gccggattct gctgatgtcg
     1861 gcctgcgacg agggcgcggg cgctccggag ttctgccgga tgctgaaaga gccccattct
     1921 taccgcgaat tcctgcaaca gattctcggc cgggaggtcg tcgtagacca gtggcaactc
     1981 gaaaaactgg cattggtgaa tctgaacgcc gggatcctgt tctacgtccc ggggctcccg
     2041 caggaatatc attccagcct gtggggccgc gctttctctt ccgcccccgc ggcgctggag
     2101 gccttgtttg aaggcctgga gccagacgcg gccatcgcgg taataccgga agggccctac
     2161 gtgctggcta aaatccggtc tccgcgtgaa gcgcaggcta tataatttct gttaagtgcc
     2221 tgaggtttgt cattccgtct gtgctttgga ctgtccggat tgttgttccg ttctggtgac
     2281 ggtggaaagc ggccgtgcca cgcggttgcg cggcaatcca gaccacccgg ttacccgggg
     2341 cttcctttgc ggtaaagtcg cccggtatct ggaccgtgaa tattcgccgg agcgcctgct
     2401 ctatcctttg cgccgtgccg gtgcgaaagg cgagggccgt ttcgaacgga tttcctggga
     2461 cgaagcgctc gccaccattg ccggagagct tcgctctata tccgaaagat tcggcccgga
     2521 agcgatcctc ccctacagct atgcgggaac gatgggctat ttgaacggat cgggcatgga
     2581 ccggcggttc tttcaccgcc tgggcgcctc ccggctggat cgaaccattt gttccgcggc
     2641 cggcggcgcg gggctggcac agaccctcgg catccggtat ggaaccgaac cggagcagtt
     2701 tcgcgaatcg aagctcatcc ttgcctgggg cgcgaacata cacggcacga acgtccacct
     2761 ctggccgttc attgtggaag cacggcgcaa aggcgcccgc ctttatgtga tcgaccccat
     2821 ccggacacgt acggccgagc tcgcggacag gcatttcgcc atctatcctg gcagcgatct
     2881 cgcgctggcc ttcggaatgg cgcacgtgat catcgccgaa gacctgtacg accacgatta
     2941 tgtggagcgc tacacctccg ggttcgagtc gctggcgcgg cgcgcgtccg aatatactcc
     3001 ggagcgtgtg gcatcgctca ccgggctggc agcggacgat atccggcaac tcgcccgcga
     3061 atatgccacc acgcgccccg cggccatccg gctcaactac ggggtgcaga ggagcgagcg
     3121 gggtggcggt gccgtccggg caattgccac acttccggca cttgccggat cctggcggga
     3181 gcccggcgga gggcttcaac tgacaacctc cggggcgttt cagatcgacc gcgatgcact
     3241 ggaaatgccg ggacttcagg aggcctctcc gcttggccgc gaggcgcgcg tcgtgaacat
     3301 ggcccggctc ggagaagctc tgaccgttct ggacaacccg cccgtgaaag cgctggtggt
     3361 gtataactcg aatccggctg ccatcgcacc taaccagaat ctggtattac gcggattgcg
     3421 gagggaggat ttgtttacgg tggtcctgga gcagttccag accgatacgg cagattacgc
     3481 cgacattgtt ctgcccgtca cgaccttcct ggagcacacg gatctttact tcgcctacgg
     3541 tcactattac ctccagcttg cacgccccgc gctggagcct cccggcgaga cgaaatcgaa
     3601 ctttgaaatt ttccggctgc tggcggagcg gctcggattc gaagagcagg cgttccgtga
     3661 aaccgaggat gatgtgatcc gggcggttct cgcggggtca aaaggcgccg tgcgaggcat
     3721 tacgctcgaa cagttggaga cggagcattc cgtgcggctg cgcttggcgg gagacggcga
     3781 tgcctttttg ccgttcgcga acggtggatt cggatctgaa gacgggaaat gctacctgtc
     3841 gatggaggat ctcgactacg tcccgcctga tgaatcgaga ttgggggagg aatcgattcg
     3901 cctacggtat ccgctggaac tggtctcgtc gaaaaacgat gacagcatga attcgacgtt
     3961 tggtcatcgt ggttttgtcg acgagcagac ttccgtgtta tggatgcatc ccggcgatgc
     4021 ctcggaacgc ggaatcgaac ctggggaaca ggttcgggtg ttcaacaacc gcggtagctg
     4081 tatactgaag gcccgaatca cggacggggt gcgtacggga gtggtaagag cgccctcggt
     4141 ccgctgggtc aagcgatccc ccggaggcaa agggatcaac gtactgacat cggaccggct
     4201 gaccgacatc ggcggcggtc cggtatttta tagctgcctg gtgcaggtgg agagatgcgg
     4261 agactagata gaaaggcaat atggattgcg acggcggcgc tggtggtggt ctcggccggt
     4321 tgctcgcggc aggagaaagt gcgcgtcgag atgacggaag aagagggtgc gcaactggcg
     4381 acgatggtcc acgtcgccga cccccgggcg aatatacagt tgctgaaggg ctttcatgat
     4441 atcgagcaaa atgcctggcg ctggaccatg tcgaagtttt cggtggcgct tcgtccgcca
     4501 gtgggtgcgg cgcaagccgg tgcgacgctt cagttgaagt tctcgattcc ggaccccgtg
     4561 ctgaagcata cgaaagacat cacgctctcg gccagcgtga acggctcccc gctgccgccg
     4621 gagaccttca aaaccaccgg ccaacatgtc tatagcaagg acgtcccacc cgctgcgctg
     4681 gcgtcggatg cgattacggt agacttcgcc ctggataagt ttctgaagcc cggcgacgtg
     4741 gatacgcgcg agctcggtgt cgttgtgacc tcggtcggcc tcgaaccgaa atgagagcag
     4801 gcgaggagcc gctcctccac aacccggcca ccgaaaccca ttccaacgct tccgctttcg
     4861 ggcgggcgct ttcacgggtg aagattggaa ccgtttttta ctggacggtt ccgccgctgc
     4921 tctgcctgct attgttccac cgcagcctga acggctggtt catgcaggac gatttcgcat
     4981 ggctcggtct gtacggcagc gtcaactcct gggaagacct gcaacgcgta ttgtttacgc
     5041 cgtacgcaca gggcacgctg cggctttgga gcgaacgggc cttctttctg gtcttttatg
     5101 ctcttttcgg cctgaacgca tttccgtacc acctttgggt gttcctgacg caagtcgcga
     5161 atctggctct gctgacccac atcacgttcc gcctgacggg ttcccgcgct gccggattcc
     5221 tcgcaccgct attttggatc accaacgccg gcattatcgt gccgatgtcg tgggcctgcg
     5281 cctataacca gatcctctgt gcgtttttcc tgctgctcgc ttttcacttc tggttgaaac
     5341 atatcgatac aggactctgg cgatattact ggtggcaagg ggccgcgttt ctgatgggtt
     5401 tcggagcact ggagatcaat gtggtatatc cggccatcgc cgccgcctat gccttactgt
     5461 gcgcccgccg ctattttgtc cggacacttc ccctgtttgc cgcttcggga gcctatacgg
     5521 tctttcacac tctggtcgcg cccaaagaca aaagcgaaat ctacgcgatc gtcatcgatt
     5581 cgaagatctt caacacgctg ctgacctact ggaactgggg tgtcgggccg taccggctgg
     5641 ccggaattac ggaagagctc ccccagtggt tcgcgcctgc cacggtcgcc gtgctgaccg
     5701 ctgccctgct cgggctggtt gtgtggagcg cattcgagcg gcgcttcctg ccggctttct
     5761 tcctgtcgtg gttcggcatc gtgattctgc ccgttttgcc gctgcgcaac cacatctccg
     5821 actactacct ggctctgccg tcgatcggca ttgccatgct gatggcctac ggtctggtta
     5881 tggcatggcg ctacggtccc cgggccatgg cggtagccat tccggtggcg gtgctgtatg
     5941 tggccagcgg ggtgcctgta atcgatgcac agacacgctg gtacgaaaag cgggggcagg
     6001 ccgtaaagaa cctgctcacc ggcgtggccc gcgcgcgcga gttgcatccg cgcaaactga
     6061 ttctactgac cggcgtatcg agcgagctgt tttggcaagg cgtttacgac cggcctttgc
     6121 ggcttctgga catcgataac gtgtacctgg ctccgggaac ggagaaatcg atcgccactt
     6181 atccggaaat ggcgcctctc tcgaattacg tcttaccggc atccatcacc ctgcgggccc
     6241 tcaaacggga aacggcagtg gtctatgaag ttcatgatgg ccggctgcgc aacatcacga
     6301 agccctatac ataccgggca ttaatggagt ttgcgaagga ggaaccccgg caggtggtcg
     6361 ccggaaacga gttctacagc ggccagcttc tagaggggtg gtactccatc gaaaacggct
     6421 accggtggat ctcgcagaaa gcatctttga ggatgggcgg tcccacacag gacggccagc
     6481 ggctgtacgt gaccggggag tgtcccgaag cgttgctcca ggcgggcccc ctgggtttct
     6541 cgatcctggt ggatggctca ccggtttatg ccggcactgt gcgtaaagcg ggcgaattcg
     6601 acgtagtggc taagcttccc ttgacggccg taggtaagga ttctgtaata atcactgtgg
     6661 aagtctcgcg cacgctctcc ccagcgggcg aaagtcgcaa actgggactg gttttcggga
     6721 ccttcgagat ccggtaactt cctcaagacg cagggttgag acgtactact cgtacctccc
     6781 gggcgttttt actgcacggc aatagtttct ggaactttct gcatacaaca gcgtcaaatg
     6841 cacttacgtc taatggcggg gacgcttgcg gccgatatca accagcggag aatacagaag
     6901 gatcgcaatg ctccggacat ggaaaggagg agaacaaata atggcacctg aattgcttga
     6961 cacctgtgct atgatgtacg gtgctggttc tttcccttcc ggtacaattc cagaaacctt
     7021 gcagtgtttc gaacgtctat tagcgatacc tggctgtggc ataaacgaat cagagagaac
     7081 aagaaaggca aaacaacaat agtgagaaga atccgcgtgc agcggatcga agaaggagaa
     7141 gtcatgacga agaccgagct caataagttc aaacaaatcc tggaagcgag actggcggaa
     7201 ctggctctcg tactgaaaaa ccgggaaggc atcgcaatcg aaaaaagtcc ggatgctctg
     7261 gacgaggttc agaacgccgc cgaacgggaa ctggcaatcc gtaatctcga ccgggaatcg
     7321 aacctcctgc gaaacgtaag ggcggcattg cgccgcatcg cggaaggaca cttcggcgtg
     7381 tgcatccact gtgaggaaga gatcagcccg aaacgtctga acgcggtacc ctgggctcct
     7441 tactgcatcc agtgccagga actggcagac cggcagcagg aaggggatgg gtccgacacg
     7501 gaagagatgc tggttaacgc ggcctaaacc ctcagaccac tttccacaaa ggcgcggatt
     7561 taaaccgcgc ctttgttgcg tctggctcct accaggctac tcgagaattc caatggcgga
     7621 gatcatcctg cctttgtctt tgtcgcgccg gtaggagtga aactcgtcac tgcaaaaggt
     7681 gcagagaggg acgatgtgga tgttgccatc gggaacaccc gccgcggcaa tttgccgccg
     7741 ggtggcttcc atcaggtcga ggtgggtttt gcgctccagc tctttaccac gctccgggaa
     7801 cagctctttg aattgaatgg ccacctcggg cccaacttca tagcagcaga cgccaatgcc
     7861 cggtccaatc gctaccacca ggcggacagg ctctgctcca aaatcgcgcc ggagccggtc
     7921 aacggctttc gcagcgaggc aggcggcggt accccgccat ccggcgtgta tcgcagccac
     7981 cgctcttact tccggatcta ccatgaggat cggctggcaa tcggccgtgc gcactcctac
     8041 gtaaataccg gcggtgttcg agaccagagc atcgccttcc ccgatatatc cggcggacgc
     8101 acccgccccc agtatccggt ccgagtggac ctgtttcacc attgccagct tcgagggcgg
     8161 aggccagaag ttcgaggcgc gtgtaccgaa accgtgatca agccacggcc actccagagc
     8221 ctcgattctg tagataccgg cggaggtctt gtgaacggcg ctcaaggttt aggcaggggt
     8281 ctgaatctgc gccaccatga cctgcttctt aaaatcttcc agcatcgatt cgtccagccg
     8341 cacctcggtg gggcgccgcg ccagcgtcat ctggtcaatc ttatcctgaa gcttcatcag
     8401 gccgtagaac aggttttcgg gccgcggcgg gcagccagtg acataaacat cgaccggaac
     8461 aatcttgtcc acgccctgta gcacggcata ggtgttgaaa gggccgccga cgctcgaaca
     8521 ggcacccatg gaaatcaccc acttcgggtc gggcatctgg tcgtagatgc gtttcaacac
     8581 cggcgccatc ttgagggtca ccgtgccggc gacaatcatc aggtccgatt ggcgcgggct
     8641 ggggcggaac acctctgcac cgaaacgggc gatgtcgaag cgtgaggcgc tggaggcgat
     8701 catttcaatc gcgcagcacg caaggccgaa agtgagcggc cacacggaag atttccgtgc
     8761 ccaattgaaa acataatcca ccgaagtggt aagaaagttc ttctcgaact ggttctgaat
     8821 gtaggacatt ggtaaggaac cgccgccgat cctaatttta tcactgacgg agctttgcat
     8881 tcattatctg atctaggatg aaagaatgca gaaacgcgca ttgggccgct ccgggatttc
     8941 tgtcgcgccg ctggttttcg gcgggaatgt tttcggctgg accgcggata ggccaacctc
     9001 gttcgctctc ctggatcgct ttgccgaaca gggtttcaac gccatcgata cggcggatgt
     9061 ctactctgcc tgggctccgg gcaattccgg cggagaatcg gagaccatcc tcggtgaatg
     9121 gctgagacgg cgcggccgcc gcgacgatct tgtgatcatg acaaaggtcg ggagctggca
     9181 gaagcataaa ggtttgaaag tctccaccat tacggctgcc gtcgaggatt cgctgcgccg
     9241 gctccagacg gactacatcg acgtctattt cgcgcacatc gacgatacga gtgttcctct
     9301 ggcggagacg atgggcgcct tctcccggtt gattcaggcg ggcaagatac gggccgccgg
     9361 agcgtcgaac tacaaagtgc aacggctctc cgaagctctg gataccgccg cctccaacgg
     9421 attggcacgc tatgaggtcc tccagccgaa ttacaatctc tatgatcgca gcgatttcga
     9481 aagtggattg gcaaaagccg cttcggacaa tgaagttggc gtcgtttgct atttcgggct
     9541 cgcggccggg tttctgactg gaaagtatcg ttccgaagag gatgtgaagg ggaagagccg
     9601 ggcaggcatg gtcaaaggct atctcaacga gcgcgggtac cggatcctaa aagcgctcga
     9661 tgagatttcc gcgggtcttt ccgcgacgca ggctcaggtt gcgctcgcct ggctgatggc
     9721 tcgtcccggg gttaccgcgc ctatcgccag cgcaacgagt ctccaccaac tggatgacgc
     9781 gctcggtgcg gcccgcctcg acctttcctc cgaagccatc gccaagctgg atgaagcaag
     9841 cgcctattga acccgctcgc ccgtttgttg tgaggcgcgc gcgtaccggt tcagcaggac
     9901 ggcgccggac tgcaatgcca cggcgatcag catccagacc tgccagtgcg agaacaaacc
     9961 ttcggagatg gcgaaagttt cggtccactg aaggtcggcg gccagccgcc acaggctcag
    10021 cacacacgcc atcagcgagg ctggagtgag cagagatgcg agcgccgagg cgactttttg
    10081 cttacggccg ggcttcttcg taactttggc gcccggcttc aggcgtatcc ggacgatcat
    10141 gtccctttag ggaagtgccg cgatggccct ggagatctcc tgggccggca gctcataatc
    10201 atgaagcttg ccttccataa agttttcgta ggatgccaga tcgaagttcc catgcccgct
    10261 caggcagaac agaatgttgc gcgctttgcc ctcctcgcgt gcggcgatcg cctcgtccat
    10321 ggcggctcga atggcgtgcg cggattccgg cgccggcaga atgccttcga tcctggcaaa
    10381 ttgcagagcg gcttcaaaaa ccggcaactg ccggtaggat tgtgcttcga tcgcgccgag
    10441 ctcgagcagg tggctggcga gcggcgacgc gccgtgatag cgcaggccac cggcgtgaat
    10501 gcccggcggc acgaagtcgt gtcccagcgt gaacatcttg atgagcggtg tcagtttcgc
    10561 aacgtcgccg aagtcgtaag cgaatacgcc cttcgtcagg gtgggacagg cggctggctc
    10621 gaccgccagc aagcgtgggc cggcgccttt cagtttctgc tcgatgtaag gcagcgcaat
    10681 accaccgaag ttgctgccgc cgccaaaaca gccaatcacc acatccggct cctcaccggc
    10741 gagccgcatc tgttcctgca cttcctgccc gataaccgtc tggtgcagca tcacatggtt
    10801 gagcacgctg cctagcgagt agttcgtatc gctgctgcct gccgcatcct cgaccgcttc
    10861 gctgatagcg atgccgaggc ttcccggcga atcgggaaac tccgccagaa cggcccggcc
    10921 ggattgcgtt ttctcgctgg ggctggcaaa cacctgggcg ccccatgcgt tcatcatgat
    10981 gcgccggtaa ggcttctgct gatagctcac cttcaccata taaacggtgc attccattcc
    11041 gaacaggttg caggcgagag cgagcgcact gccccactgt cccgcacccg tttcggtagc
    11101 aagacggcga atacccgcct gcttgttgta ccaggcctgc gcaatggcgg tattcggctt
    11161 atggctgccg gcgggactcg tgccctcgta cttgtaatag atatgcgccg gcgtatcgag
    11221 cgcacgttcc agccggtacg cccgaaacag cggcgtcggg cgccacagcc ggtaaatgtc
    11281 gagtacctcc ccgggaatat caatctcccg ttttggagag aactcctgct ctataagaga
    11341 gtccggaaaa ataggacgca agtcgtccgg cgacagcggc tggcgtgtgc ccggatggag
    11401 cggcggttcc atgggcttcg gcagcaacgg aagaacattg atccaactgg atggaatctg
    11461 attgtccctt aggaggaact tcgtaatttc ggacacggat ggctcctgaa tgataaactc
    11521 ttcattctat cgaatccact ggaaaagcaa tggacaggcg attttttctg aatctggcgg
    11581 gcagtgcggc agtttccaat ctgctccggc ctgccgcgtt tagcgcgaat gccccgagac
    11641 ccaatattct cctgatctat gccgacgatt tgggttatgg cgatctgggc tgctacgggg
    11701 caaagaacgt aacaacaccg aatctggacc ggatggcgaa agccggagtg cggttcaccg
    11761 atgcccattc gtcttctgcc acctgtacac ccagccgcta ctccatgctc accggcgaat
    11821 acgcgtggcg caagaaagga acgggcgttc tgcccggcga tgcgcgattg attatcgacc
    11881 cgaagcgtcc cacgctgccg aacatgcttc agaacgccgg ctaccggacg ggcgtggtgg
    11941 gcaagtggca tctcgggctc ggcgacggca atctcgactg gaacggcgag atcaagccgg
    12001 gaccgctcga aatcggcttt gattcggcgt ttctgatgcc cgccacgggc gatcgcgtac
    12061 cctgcgttta cgtgaaagac cggcgcgtgg tggggctcga ccccaaagat ccaatccggg
    12121 tggattatca aaaaccgatt ggcgacgaac cgacaggccg gaagaacccc gagctgctca
    12181 aaatgcatcc cagccatggt cacgacatgg ccatcgtgaa cggcgtaagc cgcatcggct
    12241 acatgacggg agggaagtcg gcgctgtggg tggacgagga catggccgcc gaattcgtga
    12301 agcgcggcat cgagtttatc gaccagaaaa ggagagaacc cttcttcctc tatttcgcca
    12361 cccacggcat ccacgtgccc cggctgccga atcgaaggtt caccggcaaa acctcgatgg
    12421 gacctcgcgg cgatgccatt gcggaactgg actggaccgc cgggcagcta ttggatcacc
    12481 tggaacggcg cgggctggcg aagaacacac tggtgatctt taccagcgac aacggaccgg
    12541 tggtggacga cggctacaag gacgatgcgg tagagaagct gggaagccac aaacccgccg
    12601 gccatttgcg cggcggcaag tatagtaact tcgaaggtgg aacacgcgtg cccatgatcg
    12661 cgcgctggcc ggaacggatc aaatccggcg tttccggtgc gctcgtgggg caacaggatt
    12721 tcttcgcatc gttcgccaca ctgtcgggcg caaaactcgg agaagacgcc gcgccggaca
    12781 gcttcgacgt attaccggca ttgctgggtg aatccaggac tggacgcgat tggattgtcg
    12841 agcatgcccg cgcgctttcc atccgccgcg gcgagtacaa gctgatcgag accaatccgg
    12901 gactggccgt cagcaaaaat accaacatcg aaatgggaac gggctccggg ccgcaactct
    12961 accgcgtgac gcaggatccg ggcgagcaga agaatctcgc cggcgccatg ccggacaaag
    13021 tgaaggagct gtcggcatta ctcgaaaaag tgaaggcgag tccgcgctct cgcgtcgatg
    13081 tccggacgcc cgtcgataac aattaataac tattgatttg cattgctttt cgcctggtgt
    13141 tggactattc tgtccctact cggagggcta tagatgtttc gaatggtcaa gctgttccca
    13201 ttgcttttta tgttcggtcg gctccaggca gccgtcattg acaccaacga tcccggcacg
    13261 atagctgctt ttcaatcggg agctacggtc gttaatttcg aaagtgtgac aggcttgacg
    13321 ccgcaagcca ttacagccta tacagccggt gatgcggtat cggccggttc cttccttttc
    13381 gatcagatca gcggcgtaca gttctcggtg ggcggtgccc cgggtacgaa catgcccgcg
    13441 ctctatcagc tttccggggc gatccagggc gatgcaacat ctccttccac ggtacttggt
    13501 cctgtcgact ttgacttcac aaccaagttc agctccggcg ccctgatcga gatctttttc
    13561 ccggtcaagg tctcgaaagt tggtttctgg ctcaatcctt cccttgggaa tgtcaatatc
    13621 attgcagcgg ataccaactt cgctttcagt caggaaacag aaacgacgct ggaaaccggc
    13681 aatgtcaccg ccgggaattt cgtaggaatt gtgagaccca cagcggatat cggcggattc
    13741 aaaatcatcg gacttggcgc aaccggcttt acaatcgacg atttcaccta tggcggtgct
    13801 tccgtcagcc ctgtccccga gcccgcgtca ctgatgctgg caggagccgg cttgcttgtc
    13861 gccgcattcc tgcggtggcg gcggcattac gcgcggtgag acgccgcaaa cctgagcggt
    13921 cgaacgttca acattcatca agctcacagg agtgaaacgg tggtggtgac gcgtctttga
    13981 tcgctctgtc atttaccccg tgctgtatgg tagattgaac ctttcgatgc aaacgcatcg
    14041 cgggcatgtc tgttcaggtt ttcctacaag gcagagtttt cgggattgag cagtttcttt
    14101 cgtcgagtct ccacgtcgcg ggcggagaga aaacggacga acgcctctta attggacggt
    14161 cgaggtgggt agctctgctt gccgaggtac tgccgcgcgc gctgctggcc gagctggggt
    14221 tgtcgcgcat gctgctcggc accagcggcg gtggccagtt tctgttggta attccggatg
    14281 aagcccgtcc cgaagcggag cgttttttgc gggccgccgc ggctgacatc agcagactca
    14341 gcaatcggat ggtcacgctc gactgggctg ccacagagaa cctgggcgat tggacattag
    14401 ttcgcaagcg gctgaatgaa gagatgcgca agcgccgctc gacacccgcg gcggatggcg
    14461 gagctgattt cttccagccc ttcccgcttg agccctcttc gacagacgcg gacgaatact
    14521 tcactttgga aatggccgtt gccatccggg aggcccaact ggtgggctgg tcgccggaga
    14581 cacccggcca ggtgctggca gacaagggta aacatacctg ggcgattgga gcctcagcgg
    14641 atgccattcc gctggcgcgg cattccgcac ccgatgatac gggagcaaga tccgcggatc
    14701 gaaacgaact tgccggacgc gccgaggggc gtgccgtgtg gggtgttcta cgcggcgatg
    14761 tcgacaactt cgatatacga atccggcggc tcaccacgat tgaagaacac gtgcagctct
    14821 cggtgatgta caaacagttc tttgccggcg aactggaaat tgtttgctcc atgccggagt
    14881 tctggcgtaa ggtgtccatc atttattcgg gcggcgacga cttcgcggtc tacggctcct
    14941 gggacggcct gattcagctt gcgcgcgaga tacagcggct gttccaccgg ctcacggaag
    15001 agaacatgaa ggattttccc ggcctggagg gcaagaccat ctccatggcc atggcgttgg
    15061 cgccggaacc cgatgcgccg cttgccgccg tgtaccagga aagcggacgg aagctcgccc
    15121 tcgccaaggc gaccgagaag cactgcattc accttctggg caggacgctc gaatggaagc
    15181 agctcgccta cgcgtcggag ctgaaagata acatgagccg catgattcgc gaattcgccg
    15241 cgccacccca gtttctgaga gagctggccg ggctgtatcg cgagacagcc gacggccgcg
    15301 atggcgctct ggagcgtccg tggcggctgc atggcaggct gaaccgagtt ctcggaccta
    15361 cacgcgacag ggacttccag cgaagccgca ccaccctgct caccgacctg gttggcaaag
    15421 gcgcatcgca aaaaagattg cggccctcgg gcagagtagc gctcgaatgg gccaagctat
    15481 ccacaggggt ttaatgatgg cagaagcggg acaggaacaa cgggaacaaa ggagatcacg
    15541 gccgcccaga gagggtcgcg aaggaggccc cgaccgtgga cgttgggaag accgtcgcgg
    15601 cgaccggggc cgtccggagg gacctccgga aatcgacctg gataaactga ttggcgacag
    15661 tctgtatctg gataatcagg cacacagcgt cgtcagccgg atgcgggaca tggattccgg
    15721 aaccaagagc cagctccgca ggttctacaa tgctgtacgg cgcgcctgcc gtgcgccgga
    15781 aaacgagcgt caacaccagt tcgtgatgct gagggcgcgt ctggcgtaca ccatcgccag
    15841 gcactccctg cgcagtctcg atacgctcga aaagctgttg ctgcaggtga cgcgcaagaa
    15901 cgacgcacgc ggctacgagc ggttccgcga cttgttcgaa gcgatagtgg cctataacga
    15961 atagcaaagg cgccgtttgc cggcgcccct gaacataccg aggaagcatg agttcgcata
    16021 cagcccagac gcaactgaca tttatcggaa agctgattat cgaaggagaa ttgcactgcg
    16081 acaccggact gcacatcggc gccggcaagg gccagctcga aatcggcggc gcggataatc
    16141 ctgtcgtgaa ggacgccttc ggcaggccgt acatcccggg cagctctctc cgcggccggc
    16201 ttcgctcgct gcttgaacaa tcgagcggca tggcgattcc ttcggaactg gtctacctgt
    16261 cgaagcggag gggacaggaa gtgcgaattc atcagagcga ccggcccgac gacgttatct
    16321 gcctgctgtt cggccgcaat ccggggagga tcgagcgcgt cagcggagag gcactggaaa
    16381 gtagcgtggc gacgccggca cggctgaccg tgtacgacgc gcctctggtg ccggaaagca
    16441 tcaccgtgca aatgcgcgag aacctggacg acgagttgac cgaagtgaag agcgagaacg
    16501 cgatcgaccg gatcacctcc caggcgaatc cgcgcacgct cgagcgcgtt cccgccggcg
    16561 cccgttttca catacgaatg gtgctggaca tcctttgcga agaggatcgg gaactgctgc
    16621 cccgcctggc tgaagggctc cgtctgctgg aagacgacac actcggcgga ggcggatcga
    16681 gaggcagcgg ccgcgtgcac ttctccggtt tgaagatggt ctggagaggg aaagaattct
    16741 attcctccgg cgcagcggag acggaactgc tggccggtgc cgatgtctcc ggactccagt
    16801 cgctggccaa cgaatccggg ctggccgaga agctcgcgta agcgccatgc ttccggcact
    16861 actcgttcga ttacggcctc gcggaccctg gcgtttcgga ccggacgacg gcgcacgcaa
    16921 tcgcgtcgac accatcggcc acagcgacac tttttattgc gcgctttccc acgccatgcg
    16981 gatgaccggc atgctggagg agtggttgga ggcgaccacg tccaccggcg gatccccggc
    17041 ggtacgcttc agctcgctgt atccgttcca gggagagact ctgtttttcg tgccgccgca
    17101 aacgctctgg ccgccgccgc cctccacgcg catccgttgg aagagcgcgc gcttcgtgcc
    17161 ggcccaggtc ctgtctcccc tgctgtccga aaagcctttg gacgaaggca ggtggacgat
    17221 cgacgcgcac agcgaatgcc tggtctcatc cgggagcacc ggcccgtttc gcgtatcgct
    17281 tcgtggagcg gcggcggtgg accgcctgga taacggaaaa acggcccctc acgccacggc
    17341 ctgtctggag ttcacggaga actccggcat gtggacggtg ttcgtattcc gtgatggaga
    17401 ggctcgcgag cgctggttgc cggcggtcca aagcgctttg cggctgctgg ccgattcggg
    17461 attgggaggc gaacgttcgc gcggatgggg aagatcggac ccgcctgaat tccgggaagg
    17521 agagtttcct tcgatcatcg cccgcatccc cgcagtggac cggactgaag accaggagga
    17581 agcggcacag aacttcgaga ccggtcactg gcttctgtct ctgttcagtc ccgcggagca
    17641 ggacacgatt gattgggatc gcggctccta ccaattagtg gtgcgtacgg gccggacggg
    17701 cggcgctcac tccggcgagc ttacgcgatc cgtcaggatg atcggcgaag gctcagtcct
    17761 tgcgggtgca gcgaagcccg ccggcacggt acgcgatgtg cgtccggaag gtgcgggcga
    17821 agccgccctg cgctgggggg tggcactgag cattccgatt cgtgtccggc gcgcacccgc
    17881 cgcgctgccg gttccacctg tcgcaccatc ggggcagccg gaaccgcaac aagtggagcc
    17941 tacaccggaa gcggagttgg caccgcagca agtggaacct gcgccagaaa cagaagtgga
    18001 accgcaacaa tcggaaccgg cggaagaacc cgaaacagcg gagtctggcg ttctctcgtt
    18061 gctctcgcct cctcccgggg aggccatgcc gcctgtggaa acgcagcagg aacccacgcc
    18121 ggaagcggaa gcgggggagc cggaatctgt cgccgtagaa ccggtaccgg agatcctgcc
    18181 ggaggaacct ctccctccgg ctgaagaggc gcaatcccag gaaccggaac cagaggagcc
    18241 ggaaaagtcc ggtgttcttg cgctgctctc gccgcctcct ggagaggcca tgccgcccgt
    18301 ggaaacacag gcggagccgg agccgatgcc ggagactcgg ccggaggaac ctctgcctcc
    18361 tgccgaagag gcgcaatccc aggaaccgga accggaggaa ccggaaaaat ccggtattct
    18421 tgcgctgctc tcgcctcctc ccggagaggc catgccgccc gtggaaacac aggaggaacc
    18481 cgcgccgcca ccggaaacat ccgatgagac cgagccccca ccatccggcc cggaggaaca
    18541 cacatgaagt accggttgac ttgcctcact cccttactgg taggagacgg acgaaggctt
    18601 tcacccatcg actacatggt gtggaaagac caggtcaatg tgctcgacca gaaccggatc
    18661 ttcaatcttc tggcaaaggg cccccggctg gaggggtacc tgatgcaatt gaagaaggcg
    18721 gacaagctgg atttcgcttc ctgggggggc ttcgcccaga acttcgccga tcgccggatt
    18781 cctttcgagc ataaaagcat gaccgcttac tgggaaaggg cgcgggcgga aaatctgttt
    18841 attcccacgt ttgcagccgg tctccaaggt ccgtacatcc ccggcagcgc actaaagggc
    18901 gcattacgca cgggcctggt cttcagccgg tggagcggta acaccttgaa agatatcgcc
    18961 ggccgcatgc agggtgaacg ctcaccccgg cggccggggg acgcggcgga ggagcaggca
    19021 atcggagcgc cggagtatag ccgcatgaaa gcggtcgcca tctccgattc ggtgccggtc
    19081 tcctatgctt cgatgaaggt ctacctgctg cgtgtggcca cgctggtcag ccgcggtgcg
    19141 ggccgttatg aactcggttg gaaacaggct cccagaggaa gtgtggacgg ccggcggccg
    19201 gaggacagca ctccgtggtt tgcggagatg gctgcgccgg gcaccacttt tgaaggcggt
    19261 ctgcacttga accaattttt ccaccagccg gagatcgtgc gggcccttcg ctggagagag
    19321 ccgcccgacc agagcacgct attccgggcg gcgaatcgct atgcagcgga tctgttggct
    19381 cgcacaaaaa gctatgcggc catggccgga ctcgggcgtg tggaagcgtc cgtcggtgaa
    19441 ctcgaaaccg aagtctcaaa agcggaggct gaaggggcat gcattctgcc tctgggctgg
    19501 ggagcgggcc tgatggcgaa gtcggcatgg ctcgatacag gcgacgagac ctacaggcag
    19561 gtaatgtccc aggtgccgct gtacgcgaag gcggtgcaaa ccggaatgcc gtttcccaaa
    19621 acccgccgcg tggtgttttt tgagaatcaa ccggctgcga tgcctggctg ggtacggttg
    19681 gaactgatgg gctgagaacg gccggttcct gtcgtgctac catgcaaata acgcgtcttc
    19741 gcgtgttcgg ccagcccctg tcatgtacaa cgccttcttc ggattcaaag aaaatccgtt
    19801 cagtttgagt cccgatcccg ccttccttta ccgcagcacg cagcatgagg aggcactcgc
    19861 caacctgatt tacggcgtac acagccgtaa gggatttatc gtgctgaccg gggaagtcgg
    19921 gacaggcaag acgactatgc tggaatgcct ccgcgatttt ctggaggcgc agcagatcga
    19981 attcgcgttt attttcaact cgcgaatcaa tgcggatcaa ttcctggaaa tgatcgctta
    20041 cgatctggac ctgaggtgca accgcacgtc caaaaccgag gtcctgttcg cactgaacca
    20101 gtttctggtg cagcaggctc accacggccg gacgaccgcg ctcattgtcg atgaggcgca
    20161 caatctggaa tgggatgttc tcgaagagat ccgcctgctt ggaaatctgg agagtcccaa
    20221 gaccggaaaa ctgatccaga ctattctggc cggtcaaccc gagttggagc gaaaactcga
    20281 cgcgccgaac ctgcgccaac tgaaacagcg catcatcctg cgatacgacc tgaaaccctt
    20341 tcgggaagca gacaccgtgg agtatatctc ctcgcgtatg gcaaaagccg gcatgcctga
    20401 gcaaaacgtt tttcccgtgg agctactttc ggaaatacac gtgaggtcgc aaggcattcc
    20461 gcgcctgatc aacgcgatct gcgacaacgc gttgctgacc tgcttcgccc tggaaactcg
    20521 tgtggcaact cttgagatcc tggatgaagt gtgcgcggat atgcgcctgg agtggcccgg
    20581 cggcaacagg cagcggggcg agcgtccgcg ccggtccggc gaatcttacg accccagcgc
    20641 ataccagtcc cgccgctaac cacctcttgc gccaaaccgc gccggtgacg gaagccggca
    20701 aggttagaat gatctcatgc gatccatggc attgcccctg ctgcttgccg tttcgttcgc
    20761 ggcggcggcc gaaccggcaa ctcacgactt gctggtgtgc ggttgggacg aggtatatat
    20821 tctgaacacc gccaccggca ggaagagctt ctcgtggaag gccgcggagc gtcccgaact
    20881 gcctgcgagt tataaagata agttccggac cacggacgaa tgcaagctcg tcgaagacgg
    20941 gcggattctg attacagcat cgagcgacgg cgtagcgctg gccgaccggc ggaccgggcg
    21001 cacgacattt tgggggcttt gcgctaacgc tcactccgcc gacctgctgc ccggagaacg
    21061 catagcagtg gcgtgctcgg taagggaggc aggcggcaac cggctggccg tcttcgacgc
    21121 acgcacgccg gagaaagaac tcttttcgac ggaactctac tccggccacg gcgccgtctg
    21181 ggatgaaacc cgcaaactgt tgtgggccct cggccatgga gaactccgag cctatgcctt
    21241 gtcagactgg gaaacgccgc gaccgtcgct gaagctggag aactcctata aacttccaag
    21301 cggtggcggc cacgaactct caccggacgg cgattcccat cttgtgattt caggttcgcg
    21361 cgacgtctgg ctcttcgacc gcgacaaccg ggaattcaag cctcatccgc agctcgccgg
    21421 gatgcatggc atcaagtcgg cgtcggtgga acccggcagt gggcgtgtgg ctttcacaca
    21481 atccgaaccc ccgaattggt ggacgtcgaa gatacagttc cgcaaccctg acggggttct
    21541 gacgcgagag ggcgaacggc tatacaaagt acgctgggtg aagcgggact aattcggaca
    21601 actcagtact ggacatttgg tttctatctt acttaggctt ctacttagcc ggtgttccgt
    21661 atcaacgaga taccggctat cccctccgta aggatatatc ttctccgtat tattccctca
    21721 gataattccg acccacctca gtttttcccc ttagactaca atgaattcgt tcgcaggatt
    21781 attagggcgt ttctcttagt ggacaccagc gaacaaagat acaaatctaa gttgaagtgg
    21841 agaacacgtt gaggattctc ggacgagtta ttttgtgtgc tacaatctgc ctgacgcttg
    21901 tgactgccat gacggcaagc gttggctcca gtcagacggt tcctgtcatc gaacctgctt
    21961 tttcatctaa cctgaatgcc actgctgtcg cgcatggacc gtttcccgct cctgatccgt
    22021 ggggtggccg agtagcccat ggaccgtttc ccgctcccga tccctggggt ggccgagtag
    22081 cacatggacc gttacccgct cccgatccct ggggtggccg cttggcgcat ggtccgtttc
    22141 ccgctcccga tccgtggggc ggccgcatcc gctcctgaac agaaactctt cgcagggctc
    22201 ccgagcaaca gtaagttcgc atctggagcc ctgccatgaa ccccctctgg aatctcgcct
    22261 ttttagctgc ccccgtcctc caggtactgt tgatctcccg cgttatcgcg ggtgcctgta
    22321 agagttttcc attagtcggt gtctatgcgg ttttcacgtt cctggccacg attgctgagg
    22381 gagcggcatt cctcggtgtt tatcctatgt ccgtcctcta cgagcggatc tattgggtca
    22441 acgaagcaat cctccagatt ctaattttct ccatcatcat ttctctcatc tatcaggcca
    22501 tgccggccac tccaagacgc aatgcagcca gccggtggtt ggtagcttcg gcggtggcgg
    22561 cgctggtgct ctccctgatt ggcagctacg acgtgaacct gaatgcctgg atgacccagg
    22621 tgagcaggaa tctggccttt gcctctgccg tattgtcgtt cctgctctgg accctcctgc
    22681 tgagccggca ttcgcgaaat acgcaactgc tgctggtcag cgccgccttt ggcatccagg
    22741 ccaccggcaa ggctcttggc cactccctcc gctatcttaa attcgtctcg ttcggtgatg
    22801 tcgtgatgat cctcacctac ctggtgagcc tgttcgtatt gtggaaggcg ctcggaattt
    22861 cccgggcatc ggaggagcgg gcagtccgtt ccggtcacac tgttgtttga cacgtattct
    22921 cgcccaccct tacactgaat tttgatttag aggagctgat ccgctcacag atgctacttg
    22981 cgagagagtt tatcgcctac ctgtcccgcc agatcgtaac gaaattaaca ccgcaaattt
    23041 tggaagtcgc ctccgcagag tccgcagcgg ccatgatcgg cgacatcatc accgaagagc
    23101 tcgcagtaga ggacaagctc aacgacgagg ttcgcgagat cctgagcgaa tatagcgaat
    23161 acatgcgtcg cgagggcgtc agctaccagg agatgttccg ccgcatcaag aacacgctca
    23221 tcacgcagcg gaaggtgata cgggcttcgg gccgggatac cggcgaccag atgaagctgt
    23281 cgcgggacaa aatcaacgat ctttcccaca agctggtaac cgccatccgt aagtcgcgcg
    23341 agttccgcct gaagcgcgac ccgaatgagg tccggctgga aattgtccgt gccatctccg
    23401 agctactgac cgtggaagag aaggtagacc gcgtggcccg gcagaaaatc cgttcccaga
    23461 agcgggagat tcccgagggc acggaggagt gggacctgct gcacaaacgc tactatgccg
    23521 aggagttgaa gaagttcggc atagaactat cccgatgaag cccaccgtct gcgtcgttgg
    23581 cagtctcaac atggattttg tcgtgaaggt ggaccacctt ccggctcccg gcgaaaccgt
    23641 tctcgggcgg gactttcaga tgtttccggg aggcaaaggc gccaaccagg cgtgcgccgc
    23701 gggaaaactt ggctccccgt cggtagacgt gcggatggtg gggcgggtcg gctatgacgc
    23761 ctttgccgat cacctgaagg ccagtctgtc ggcggccgga gtggacgtca gctacgtgca
    23821 cgcgacgcga tcccaaccca ctggtatagc cctgatctgg gtggaatcgg ccggacagaa
    23881 ctccattgtg gtggccgcgg gcgccaacaa cgaactcgcc gcctcggagg tggaagccat
    23941 gcgcgcggcg atgcgcgatg cccggtatgt gctgtttcaa ctggagacgc ccctcgatac
    24001 ggtggaagcc gcgctgaagc tggcccgcga ggagggagca aaaaccattc tcgatccagc
    24061 cccggcgcag cccctctccc tggatttgct gcaactggtc gacatcctca cgcccaatga
    24121 aaccgaagcc tgcatcctgc tcgggcacgc tgttggacga gtggagttga acgatgtgcc
    24181 ggtcctggcg aatcagcttc gctcgatggg cccgcggtcg gtggtgctga agctgggaga
    24241 aaagggctgc tactacgacg acggcgtcaa acagatccac agccccggct tccccgtaaa
    24301 agcgtgcgat acaacggcgg cgggtgacac gttcaacggt gcgctggcca tcgcgctcgc
    24361 agaaggccag gatatcgaga cggcactgcg tttcgccaac tgcgcggcgg ccatctctat
    24421 cacgcggaca ggcgcccagg cttccatccc ctcacgaatg gaagtcgacc ggttttccgg
    24481 ctaatcagcg aatgcggtaa acaagaaact gttcgttctc gaacaccgga cggcggtccg
    24541 gcagccggtg ctccggcttc agcaccaggt actcaattcc caatccggcg aactccggga
    24601 cccgggcggg gtcaaatttg gaagccatgg tctgccgcca tcgctcccac cactcctcgc
    24661 ccacgttctt gtgatagttc acctgcccgc cggctttcca gcacacatag acaggccgaa
    24721 tcgcgcgcgc gcgaaagatg cccggggcga gatctctcgc ggcatcggca aacaggaaaa
    24781 tcgaatcctt cgccgtttcc gtgcgcgccc attgggagag ttgctgaagc ggctctgtgt
    24841 gcagagccgg gaaattcacc acccgcgcgt agcccggcat ggcccagaag ggtgcgcagc
    24901 ataacaggag gagtacggcc gaagcccatt tgcgctttcg tgcatccgcc caggctgcca
    24961 aaacacctat tgccgccagg acgatcatca cggtgaatcg tctcagcaag gtgacattcg
    25021 agaaatcggg ccacaacaga tccagcacac gcgtattgag cggcacggca taagcggccg
    25081 caaaccagag gaagctttcc caccagatgc ccttttgagc ggcatgaatc gccgcagccg
    25141 aagagagaat caccgcgaac aaggtcacaa acagcagggc gcgggcgggc tggtactgtg
    25201 gaatcaaaat ccacttcatc ttttcgagca aaagataaga cagcgggacc gacacgatgc
    25261 ccagcaacgg caggcccagc acgaagggtt tcaaagccgg ggccataacg gagcgaagcc
    25321 ggaaataagc ggccgcaatc gccgctgtga agaagagata ttggccgtac cactgcccga
    25381 tccacagcga cacccaattg taagaagcgc gcattcgttg caaagcttcg agctccgggt
    25441 cgatgcggcc cagaaagacc tgtggttccg tgactccggg ttgtagccgc gacagcacga
    25501 aaatcaggag tacggcg
//
LOCUS       DASTMK010000031        10618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_258268, whole genome shotgun sequence.
ACCESSION   DASTMK010000031 DASTMK010000000
VERSION     DASTMK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258268"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..150)
                     /locus_tag="VFQ79_01145"
     CDS             complement(13..150)
                     /locus_tag="VFQ79_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138280.1"
                     /translation="MELDLIVTAFPQGGEIPRLHTCDGADLSPPMEWSGEPLGTKGFA
                     Q"
     gene            complement(195..1289)
                     /locus_tag="VFQ79_01150"
     CDS             complement(195..1289)
                     /locus_tag="VFQ79_01150"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU0138281.1"
                     /translation="MRVAGIMSGTSLDGIDVAIVEIRGRRWELLEHYTVPYTQKVRSA
                     LLSISNAGTHTATISRLHFLLGELYARALGDRKVELVGCHGQTVFHEGTAVSFLGAKV
                     ASTLQIGEAALIAERTGVPVVSDFRPRDIAAGGKGAPLVPYVDYFLFRHPLRGRVALN
                     IGGIANITAIPPGAKPEGVIAFDTGPGNMVIDALAAEHTGGKQRFDRGGRIAAGGAVD
                     RKLLGRLLRAPFYKVKPPKTAGREQYGREFVAELLAAGRALPDLIATATALTAAAISE
                     GIRRFVPFRVDDLIVSGGGAHNPVLMGYLAAFLPETAVQISSDFGIDIDAKEAIAFAI
                     LAYETWHRRPSNLPSATGARHPAILGKITQ"
     gene            complement(1286..3877)
                     /gene="mutS"
                     /locus_tag="VFQ79_01155"
     CDS             complement(1286..3877)
                     /gene="mutS"
                     /locus_tag="VFQ79_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU0138282.1"
                     /translation="MQQAATPLMRQYHAIKQQVPNALLLFRLGDFYELFYEDAVTAAR
                     ELEITLTSRNKEKGEAIPMCGVPHHSAESYLARLIQKGYRVAICEQMEDPKLTKKIVK
                     REITRIVTPGTATDAAVLRSGENNYLAAITVDGGRAGLAYVDVSTGEFRTTELDTADV
                     VPALENLNVREVLAARPVPLSGGMLRTEMEGWVFESDYGERTLCDHFGLHSLDGCGLS
                     GRHLAVGATGAILHYLRDTQKSTLAHLERPAFFDRSDAMVLDSVTVRNLELVEPLFSA
                     DQKDSTLLSVLDETSTGMGARLLRRRLLRPSVSREEIEARLDAVEELAGATITRSELR
                     KVLPAILDLERLLAKVTLGTAGPRELLALGRSLDRIPALKALAKECRSERLRMLHDGL
                     DEVADVRGRILRGLSDEPPVSLSDGGTIRDGFNAELDELRDIRRNSRQHIAQIETRER
                     TRTGIQSLKVRFNNVFGYYIEISKSNLHLAPSDYDRKQTLVNAERFTTPELKELESKI
                     LEAEERMLAIEKSIFAEIRDFAAEHAARIRQTAAAIAELDVTASLAHVAAANRYARPA
                     FSSGGEMQVAAGRHPVIEKLTEREAARFIPNDVYLDPGGEFIALITGPNMGGKSTYLR
                     QAALIAVMAQMGCFVPADTALLPIVDRVYTRIGASDNLARGRSTFMVEMTETAVILNT
                     ATKNSLIVLDEIGRGTATYDGLALAWAVIEHIHQRVGAKTLFATHYHELTELAGQLSG
                     VRNLHVSVKEAGDQIIFLRKVEPGSADRSYGIEVARLAALPISVIERAREVLALHEKS
                     EQEVTEELTPRRRRSEPMQIQLFEPVNYQIAARIRNLNLDELRPIEALQLLSELQKEL
                     GKQHGET"
     gene            complement(3877..5151)
                     /locus_tag="VFQ79_01160"
     CDS             complement(3877..5151)
                     /locus_tag="VFQ79_01160"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU0138283.1"
                     /translation="MLLEATPPLEQELNPWQSADARFKEAAARLGLDDGMCKILSTPA
                     REITIHIPVQLDDGRLEVFTGYRVQHSVARGPGKGGIRFAPDVTLDEVRALASWMTWK
                     CAVVNIPFGGAKGGVICDPSVLSQFELERITRRYTAEILDFIGPERDVPAPDMNTNEQ
                     TMAWIMDTYSMHHRTTVNAVVTGKPLDLGGSRGRPEATGRGCLIVTLQALRRLGLEPS
                     ATRVVIQGFGNVGGMAAKLMQRAGFKIICIIEVDGAVYNPKGLDVLALMEHRKETGSI
                     TGFPEGEDIDKDDALFLDCDVLLPAAKENVITSQNADRIRAKILCEGANGPTTAVADS
                     ILADKRVFVIPDILANAGGVTVSYFEWVQDRQGFFWNEQLVNGRLEEIMVNSFRDVMG
                     YAEKHQVHNRTAAYMLALDRVAFAIKLRGIYA"
     gene            5235..6038
                     /locus_tag="VFQ79_01165"
     CDS             5235..6038
                     /locus_tag="VFQ79_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138284.1"
                     /translation="MVTRRDFLVLCACWFPFFWRRRCIRMAGISFRVIRRGSGTRRYL
                     HIHGNEATARELLFSHLQTFKGTGFVIESEQRYVVVRGGWIDPNRMFSTTGAEKSLRA
                     LNPHWSGEDVRAALQELDRDREKFLHAIAPPRGGLLIVLHNNSETYSVQDEAPISDRV
                     SLKASASPREFLLATDPRDFEILSASPFNVVLQNTPAGEDDGSLSRWAAREGVRYVNI
                     EAALGNRQGQAEMLEWLEKNLPPVYGPAAVSLLRRKPEWFPDQTWSGIT"
     gene            complement(6014..7393)
                     /locus_tag="VFQ79_01170"
     CDS             complement(6014..7393)
                     /locus_tag="VFQ79_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138285.1"
                     /translation="MPSTSRRSFVRNAALGAGLAGAPAVRPSWGQNSPNNQINLGVVG
                     FRGRGRDHYRAFARIPGVRVAYLCDVDERLFPGAVAEVEKIGGNKPATFVDLRKLLQQ
                     KDLDAISIATPDHWHALQTIWACQAGKDVYVEKPVCHNLREGRKMVEAARKYNRIVQV
                     GLNRRSELRNKAGITFVREARFGPAYRAKSVVYRGRTSIGHVQESSIPNGVHWDLYLG
                     PAPYRAFTLNRFHYGWHYFWDTATTELGNNGVHTIDVVRWALNKNVHPVKIHCNGGLF
                     AEDSDQQTPNVQNATFEYADGTLVDLEATTLPSPTFGGVHMGEFFYTPQGYITSAQKW
                     STVIGEFTPGNTPDPPSGISLRASNLSFPKIAYKPGPPIPDIEGPEVSHFQNFIDCVR
                     SRKREDLHCEVLEGHMSTALCHLANISFRTGRKLTFDPATETFPGDAEANKYLTREYR
                     EPYVIPDQV"
     gene            complement(7501..8358)
                     /locus_tag="VFQ79_01175"
     CDS             complement(7501..8358)
                     /locus_tag="VFQ79_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEU0138286.1"
                     /translation="MAFCTHCGARLQDDARFCAGCGQATGPAATAAPAAVEPLAYTIQ
                     GDNLQVARVKLKPEQEIYAEAGKMLYKTSNVQWETRMSGDTLGAKLWGAVKRKFMGES
                     LFMTYFRSLGEGEVGFAGSYPGRIQVFDLAAGQSILAQRDSFLFAQSTVQLNIALVKK
                     LGAGFLGGEGFILEKLTGPGSVFIHGGGDFIEFQLGPGEQLQVDTGCIVAFDESVTYD
                     IQLAGGIRTAIFGGEGLFLATLTGPGRAIVQSMTLEKMRRELAPAQSGGDERGPLGSL
                     TGLLSSEDR"
     gene            8540..9046
                     /locus_tag="VFQ79_01180"
     CDS             8540..9046
                     /locus_tag="VFQ79_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU0138287.1"
                     /translation="MKSSQTHAADAELGSANGSAPVSVGNYKVFNRIVDWITSDRMQL
                     LNITDRINEIVRRSGVRDGIVHLQSLHTTTAVFINEWQDALIQDVKSFFDQVVQRDQY
                     YRHNDPDYSDCERRNADSHLRGMMMGQTVCLQVRNASVLLGTWQSIIFAEFDGPRSRS
                     LAVQISGV"
     gene            9057..9494
                     /locus_tag="VFQ79_01185"
     CDS             9057..9494
                     /locus_tag="VFQ79_01185"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEU0138288.1"
                     /translation="MSRFFSLRQAESLLAMLEPAVRRAITLKTAFQEADEQLDGIAKR
                     ISALGGARVNQEEALSLRNRRDSAGSRLKDCIEEIQATGCQIKDLDIGLLDFPTLYRG
                     EEVLLCWKLGEEGIRFWHNLTEGFRGRKPIDEDFCRNHAGDPE"
     gene            9564..9639
                     /locus_tag="VFQ79_01190"
     tRNA            9564..9639
                     /locus_tag="VFQ79_01190"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9597..9599,aa:Ala,seq:ggc)
     gene            complement(10165..>10618)
                     /locus_tag="VFQ79_01195"
     CDS             complement(10165..>10618)
                     /locus_tag="VFQ79_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138289.1"
                     /translation="EKLRAEFTKSRACRSQDNALVEGKNGAVIRKLMGYGYIAGEHAE
                     AIGTFYAHYLNPYLNYHRPCGFATVSLDERGKRRRKYRTEDYRTPFEKLKSLPEVEKC
                     LKPGVSLPEMEREALAMSDTECARRMSAAKAKILRQCKAQSRVPPRFR"
BASE COUNT         2055 a         3163 c         3141 g         2259 t
ORIGIN      
        1 ttaagttaac gcttattggg caaacccctt ggtgccgagc ggctcgccgg accattccat
       61 cggaggcgac aggtccgcgc catcgcaagt atgcaaccgc ggaatttcgc ctccctgcgg
      121 aaacgccgtc acaataagat ccagctccat attcctccga tcaactcgct gctcccggga
      181 ctaatcttac cgcttcattg tgtgattttg ccgagaatcg ccggatgcct ggctcccgtt
      241 gcggaaggca gattcgacgg gcggcggtgc caggtctcat aggcgagaat cgcgaatgca
      301 atcgcctctt tcgcgtcgat gtcgattccg aaatcactcg atatttgtac tgccgtctcc
      361 ggcaaaaaag ccgcgaggta acccatcaaa acgggattgt gagcaccgcc gccggagacg
      421 atcaggtcgt ctacgcgaaa tggaacgaac cttctgatgc cttcgctaat cgcggcggcg
      481 gtcagagctg ttgcggttgc gatcaggtcc ggaagcgcgc ggccggcggc caacagctca
      541 gccacaaact cacgtccgta ttgctcccgg cccgcggtct tgggaggctt gactttgtaa
      601 aacggagcgc gaagcaggcg gcccagcagt ttcctgtcaa cggctccgcc ggccgcgatg
      661 cgtccacccc tgtcgaaacg ctgtttgccg ccggtgtgct ctgcggccag cgcgtcgatc
      721 accatgttgc cggggccggt atcgaacgca atgacccctt ccggtttcgc gccgggagga
      781 atggccgtga tattcgcgat gccaccgatg ttcaatgcga cacgcccgcg cagggggtgg
      841 cgaaaaagaa aatagtcaac gtaagggacg agcggcgctc ccttgcctcc cgccgcaata
      901 tcgcgtggcc gaaaatccga aacgacggga acgccggtac gttcggcgat gagcgccgct
      961 tccccgattt gcagagtgct ggcgaccttg gcgccgagaa aagaaaccgc ggtcccttca
     1021 tggaataccg tctgcccgtg gcagcccacc agttctactt tccggtcacc gagcgcgcgc
     1081 gcgtacagtt cgcccaacag aaaatgcagg cgggagatcg tcgccgtgtg cgtgccggcg
     1141 ttggatatcg agagcagcgc cgagcggacc ttttgagtgt agggtaccgt gtagtgctcc
     1201 agcagctccc aacggcggcc tcgaatctcg acgatagcca catcaatccc gtcgagcgac
     1261 gtgccgctca tgatgcctgc gactctcatg tctctccgtg ttgtttgccc agttcttttt
     1321 gtaactcgct cagaagttgc agtgcctcaa tagggcggag ttcatccagg ttcaggtttc
     1381 ggatgcgggc cgcgatctgg tagttgacgg gctcgaatag ttgaatctgc atgggctcgg
     1441 agcggcggcg ccggggcgtc agttcctccg tcacttcctg ttcggacttt tcgtgcaggg
     1501 ccaggacctc ccgcgcccgc tcgatgacgc taatcggcaa agccgcaagc cgcgccacct
     1561 cgataccata gctacggtcg gcgctgcccg gctccacctt gcgcaggaag atgatctggt
     1621 cgccggcctc ttttacggag acgtgcaggt ttctcacacc ggaaagctgt ccagccagtt
     1681 ccgtgagctc atggtagtgt gtcgcaaaca gcgttttggc tccgacgcgc tgatgaatgt
     1741 gctcaatgac ggcccaggcc agcgctaaac cgtcgtaagt tgccgtgccg cgcccgatct
     1801 cgtccagaac aattaagctg ttctttgtcg ccgtgttcag gataacggcg gtctccgtca
     1861 tctccaccat gaaggtggac cggcctcgcg caagattatc cgaagcgccg attcgcgtat
     1921 agacgcgatc cacgatggga agcagcgcgg tatcggcagg aacgaagcag cccatttgcg
     1981 ccatcacggc aataagcgcg gcctgccgca aatacgtgga cttgccgccc atgttgggac
     2041 ccgtgatgag cgcgataaac tcgcctccgg gatcgaggta aacgtcgttc gggataaacc
     2101 gggctgcctc gcgttcggtc agtttttcga ttaccggatg acgtccggct gcgacctgca
     2161 tctcgcctcc agaggagaac gccggccgcg cgtagcggtt cgccgctgcc acgtgggcca
     2221 gagaggccgt cacatcgagc tcggcgatag cagcggcggt ctgccgtata cgcgccgcat
     2281 gttccgccgc aaaatcgcgg atctccgcga agatgctctt ctcgatggcg agcatccgct
     2341 cctccgcctc gaggatcttg ctttcgagct ccttcagttc gggagtggtg aaccgctcgg
     2401 cgttcaccag cgtctgcttt cggtcgtaat cggagggagc cagatgcagg ttcgatttcg
     2461 agatttcgat gtaatatcca aaaacgttat tgaaccggac cttcagcgat tgaatgcctg
     2521 tccgggtccg ctcgcgcgtt tcgatctggg cgatgtgctg gcggctgttg cgccggatgt
     2581 cgcgcagttc atcgagttcg gcgttgaatc catcgcggat cgtcccgcca tcactcaagc
     2641 ttaccggcgg ctcgtcggag aggccccgca aaatccggcc gcgcacgtct gcgacttcat
     2701 ccagcccgtc gtgcaacatg cggagacgct ccgagcggca ctccttcgcc agcgccttca
     2761 gtgccggaat ccggtcgagc gaccgcccga gtgccagcag ttcgcgcggc ccggccgtcc
     2821 ccagagtcac ttttgccagc agccgttcga ggtcgagaat ggccggcaga acctttctca
     2881 attcggatcg tgtaatcgtc gcacccgcca gttcctccac ggcatccaga cgggcttcga
     2941 tttcctcgcg gctgacggat ggccggagca gcctgcgccg gagtaatcgc gctcccatgc
     3001 cggtcgaggt ttcatcgaga acgctgagca gcgttgaatc cttctggtcc gcgctgaaga
     3061 ggggctctac gagttcgagg tttcgcaccg tgaccgaatc gagaaccatg gcatccgagc
     3121 gatcgaaaaa cgcgggccgc tccaggtgag caagagtcga tttctgcgtg tcgcgcaggt
     3181 agtgcaggat ggccccggta gcgcctacgg caagatgccg gccggatagc ccgcagccat
     3241 cgagagagtg cagcccgaaa tgatcgcaga gggttcgctc tccgtagtcg ctctcgaata
     3301 cccagccctc catctcggtc cgaagcatac cgccggaaag cggcacgggt cgcgccgcca
     3361 ggacttcgcg gacattcagg ttttcgagcg ccggaactac atcggccgtg tccagttccg
     3421 tagtccggaa ctcgcccgta gatacgtcga cataggccaa ccccgcccgc ccgccatcaa
     3481 ccgtaatcgc agccaggtag ttgttttcgc cggagcgcag cactgcggcg tccgttgcgg
     3541 ttcccggcgt tacaatccgc gtgatctcgc gcttgacaat tttctttgtg agcttcgggt
     3601 cttccatctg ctcgcagata gcgacccggt atcctttttg aatcaggcgg gcgagatagg
     3661 attcggcgga atgatgcggc accccgcaca tggggatcgc ttcgcccttt tctttgttcc
     3721 gggaggtgag ggtgatctcc aattcccggg cggcggttac agcatcttca tagaagagtt
     3781 cgtagaaatc tcccagccgg aacaacagca gcgcgtttgg aacctgttgc ttaatcgcgt
     3841 ggtactgccg catcagcggc gttgcagcct gctgcatcac gcgtaaatgc cgcgcagctt
     3901 gatcgcgaaa gcgaccctgt ccagcgcgag catgtaggcg gcggtgcgat tatggacctg
     3961 gtgtttttcg gcgtagccca tgacgtctcg aaaactgttc accatgatct cttccagccg
     4021 gccgtttacg agttgctcat tccagaaaaa gccctgacgg tcctgcaccc attcgaagta
     4081 ggaaaccgtc acaccgccgg cattggccag gatatcggga ataacgaaca cccgcttatc
     4141 ggcgagaatc gaatcggcga cggcggtcgt ggggccgttc gccccttcgc agaggatctt
     4201 ggcgcggata cgatccgcgt tttgcgaggt gataacgttt tctttggcag cggggagcag
     4261 aacgtcgcaa tccagaaaca gagcgtcgtc tttatcgata tcttcgcctt ccgggaaccc
     4321 tgtgatggag cctgtctctt tgcggtgttc catcagggcc aacacatcaa gacccttcgg
     4381 gttgtacaca gcgccgtcca cttcgatgat gcagatgatc ttgaatcctg cgcgctgcat
     4441 cagcttggcg gccatgccgc cgacattgcc gaatccctgg atcacgacgc gcgtggcgga
     4501 cggttccaga cccagccggc gcagagcctg cagtgtgaca atcaggcatc cgcgtcctgt
     4561 ggcttcagga cggcccctcg atccgcccag gtccagcggc ttgccggtaa ccactgcgtt
     4621 caccgtggtc ctatgatgca tggagtaagt gtccatgatc cacgccatgg tctgctcgtt
     4681 ggtgttcatg tcaggcgccg gcacatcgcg ctcgggcccg atgaagtcca ggatctcggc
     4741 cgtgtaacgg cgggtaattc gctccagctc gaactgcgac agcacggagg ggtcgcaaat
     4801 aacaccaccc ttcgctccac cgaatgggat gttcaccact gcgcatttcc acgtcatcca
     4861 ggaagctaat gcccggacct cgtccagcgt aacgtccggg gcgaaacgga tgcccccttt
     4921 tcccggacct cgcgcgacag agtgttgtac tctgtatccg gtgaatactt caagccggcc
     4981 gtcatccaac tgaacaggga tgtggattgt gatctccctg gcgggggtgc tgagaatttt
     5041 gcacattcca tcgtcgaggc cgagccgggc ggcggcctcc ttgaatcgcg catcagcgga
     5101 ctgccaggga ttcaactcct gttctagcgg cggagtggct tccaagagca tgaactttcg
     5161 ttcctccgcc tacattatga caccgaaaac ccgttacatc cgtcatttca atcgtgggtg
     5221 aaagaagcgg ccgaatggtc acgaggcgtg attttctcgt gctgtgcgcc tgctggttcc
     5281 ccttcttctg gcgccggcgt tgtatccgta tggccggcat ttcgttccgg gtgatccggc
     5341 gtggcagcgg gacgcgaaga tacttgcata tccatgggaa tgaagctacg gcgcgagagc
     5401 tgttattcag ccaccttcaa acctttaaag gcaccggctt cgtcatcgaa agcgaacagc
     5461 ggtacgtagt ggtgaggggc gggtggatcg atccgaaccg gatgttttca actaccgggg
     5521 ccgaaaagag tttgcgggct ttgaacccgc actggagcgg cgaagacgtg cgcgccgcgc
     5581 ttcaggaact cgaccgcgat cgtgaaaagt ttctccatgc gattgcccct ccccgtggag
     5641 gcctactcat cgtgctgcac aacaacagcg aaacttactc tgtacaggat gaagcgccga
     5701 tcagcgaccg cgtgtccttg aaagcttccg ccagcccgcg ggaatttctg ctggccaccg
     5761 atccgcggga tttcgaaatt ctcagcgctt cgcccttcaa cgtcgttttg cagaatacac
     5821 cggccggcga agacgatggg tcgctgtcac gttgggccgc gagagaaggc gtccgctacg
     5881 tcaacattga ggctgcgctt ggaaacaggc aggggcaggc ggagatgctg gaatggctcg
     5941 agaagaacct gccgccggtg tacgggccgg cggcggtcag ccttctacgc cggaaaccgg
     6001 agtggtttcc agatcagacc tggtccggta tcacgtaagg ttccctgtac tcacgcgtga
     6061 gatacttgtt ggcttccgca tcgccgggaa atgtttccgt tgcgggatcg aaggtcagct
     6121 ttcttccggt gcgaaaggag atgtttgcga ggtggcacaa tgcggtggac atgtgccctt
     6181 cgagaacctc gcagtgcagg tcttcccgct tgcgcgaccg cacgcaatcg atgaagttct
     6241 ggaaatggct cacctcgggt ccttcgatat ccggaatggg gggtccgggt ttgtaggcga
     6301 tcttcgggaa gctgagattg gatgcgcgca acgagatgcc ggacggcgga tccggcgtgt
     6361 ttccaggggt aaactcgccg attaccgtgc tccatttctg ggccgaggta atgtagccct
     6421 gcggcgtgta aaaaaactct cccatatgca cgccaccgaa agttgggctg ggcagtgtcg
     6481 tcgcttccag gtccaccagg gttccatccg catactcaaa ggtggcgttc tgtacgttgg
     6541 gggtctgctg gtccgaatcc tccgcgaaca gaccgccgtt gcagtggatt ttcaccgggt
     6601 gcacattctt gttcaacgcc caccgcacga cgtcaatggt atggacgccg ttgttgccga
     6661 gttccgtcgt agccgtatcc cagaagtagt gccatccgta atgaaaccgg ttgagagtga
     6721 atgcgcgata cggtgcgggc ccgagataga gatcccagtg cacaccgttc ggaatcgaag
     6781 actcctgaac atgcccgatg cttgtgcggc cgcggtacac gaccgacttc gcgcggtagg
     6841 ccggtccgaa ccgtgcttcg cggacgaagg taatgccggc cttattgcgc agttcactgc
     6901 ggcggtttag acccacctga acgatgcggt tatacttgcg ggcggcttcc accattttgc
     6961 ggccttcgcg caggttgtga cagacgggtt tctccacgta cacgtctttg ccggcctgac
     7021 atgcccagat ggtttgtagc gcatgccagt ggtccggagt ggcgatcgag atcgcgtcaa
     7081 ggtccttctg ctgaagcagc ttccgcagat ctacgaacgt ggccggctta ttgccgccga
     7141 tcttttcaac ctcggcgacg gcgccgggaa acagacgttc atcgacgtcg cacaggtagg
     7201 cgacgcgcac tccgggaatt cgagcgaagg cgcgatagtg atcgcggcct cgtccccgga
     7261 aaccgacgac tccgagatta atctgattgt tcgggctgtt ctgaccccac gacggacgga
     7321 cggcgggcgc acccgccaat cccgcgccca aggccgcgtt tcgtacgaaa ctgcggcggc
     7381 tggtagacgg cataatggca agacctcctg attgtgaaat gcttgaaatc tcattgtgcg
     7441 gtgtccgggg ggcaggagtc cagccggtcg ggcttcgggt tatttgcaga ctactcgcag
     7501 ctaacgatct tcgctgctca gcagcccggt gagcgaaccg agaggaccac gttcgtctcc
     7561 tccgctctgg gccggcgcca gttcccgccg catcttctct aatgtcatgc tctgtacaat
     7621 ggcccgcccg ggtcccgtaa gcgttgccag aaacaaacct tcgccgccga agatcgcagt
     7681 tcgaattcct ccggcaagct gaatgtcgta ggtgacggat tcatcaaacg ccacgataca
     7741 acccgtatcc acctgcagtt gttcgccggg cccgagttga aactcgatga aatcgccgcc
     7801 gccatggatg aatacgctgc ccggtccggt aagtttctcg aggatgaagc cttcacctcc
     7861 cagaaagccg gcaccgagct ttttcaccag ggcgatattc agttgcacgg tcgattgggc
     7921 gaacagaaaa ctgtcgcgct gcgcgagaat gctttgaccc gccgccagat caaacacctg
     7981 aatgcggccg ggataagatc ccgcaaaccc gacttctccc tctcccaggc tccggaagta
     8041 cgtcatgaaa agggattcgc ccatgaactt ccgtttgacc gcgccccaca gcttcgcgcc
     8101 aagagtgtct ccggacatgc gggtctccca ctggacgtta gaggtcttgt acagcatctt
     8161 tcccgcttcg gcgtagatct cctgttcggg cttcagcttt acacgcgcga cctgcaggtt
     8221 gtcgccctga atggtgtaag cgagtggctc gactgcggct ggggccgctg ttgccgccgg
     8281 tccagtcgct tgtccgcatc ccgcgcagaa cctggcatca tcctgtagac gcgccccgca
     8341 gtgggtacaa aaggccatta gatcgctatg ctcctcaagg aatgtatctt agcatcgggg
     8401 gcggcgcgtt atcgctggtt ccggctgttg aaagacaggg taatccccac cgctggtata
     8461 atcgaagcgt tactttgcgg gcaattcgcg taaatcgttc aatcgagatc catcatttca
     8521 tcgagaaggg acttgggtga tgaaatcctc acaaactcat gctgcggatg cggagctggg
     8581 ctccgctaac ggttcggcgc ccgtgagcgt cggtaattac aaggtgttca acagaattgt
     8641 tgattggatc accagcgacc ggatgcagtt gctgaacatt accgaccgca ttaatgaaat
     8701 tgtccgccgc agcggcgtgc gcgacggcat cgtccacctg caatctctgc ataccactac
     8761 agccgtcttt attaatgagt ggcaggatgc gctgattcag gatgtgaagt ccttcttcga
     8821 ccaggtggtc cagcgcgacc aatattatcg ccataacgat ccggactact cggattgcga
     8881 acggaggaac gcggattcgc acttacgggg catgatgatg ggacagaccg tgtgccttca
     8941 ggtacggaac gcttctgtgc tgctcggaac ctggcagagc atcattttcg ctgaattcga
     9001 tggaccgcga agccgctcgt tagccgttca gatttcgggt gtgtgatcgg agtcagatgt
     9061 cgagattttt ttccctccgg caagccgaat cgcttctcgc catgctcgaa ccggcggtcc
     9121 gccgcgccat tactctgaaa acggcttttc aggaagccga tgagcagttg gacggcatag
     9181 cgaaacggat ttctgcgctt gggggcgccc gcgtgaatca ggaggaagcg ctgtcgcttc
     9241 gaaaccggcg cgattcagcg ggctcgcggc tgaaggattg catcgaggaa atccaggcaa
     9301 ccggctgcca gataaaagat cttgacatcg gattattgga ctttccaacg ctctatcgcg
     9361 gcgaggaggt tcttctttgc tggaaactgg gcgaagaagg cattcgcttt tggcacaatc
     9421 tcaccgaagg ttttcgcggc cgcaaaccga tcgacgaaga tttctgccgc aaccatgcgg
     9481 gcgatccgga gtgagattgg aaggtgcaac ggtgcaacgc accggcgtgc ggctatgcta
     9541 tattagcgtt gtcgaccccg cgtgggcctg tggcgcagtt gggagcgcgc ttccatggca
     9601 tggaagaggt cgtgagttcg aatctcacca ggtccaccaa aacatccccc aacatttcaa
     9661 aaacttagac gccggtcgtg tgtctgcact cctcgacgat ccactccgtg aggtccaaat
     9721 gaggtccact cagggtccac gccgcgacct tccgttggta tccatggacc gcagatgagt
     9781 accgcccgag cctgctgaac tgccgcgtgt tggtatccat ggtggccgtg gtagacatcg
     9841 ccctgccccg cttcgtccag cacgaagaaa agggtgctct tatatagcag atcgccccga
     9901 cagcattttc caattgaaag atgagcatga aatgggttct gagcggcgga agtcgaagcg
     9961 gtaggggaag gacgaggccg cgtagcggcc gaggtccttt ctttgatttt ggatttgaat
    10021 gagaagccgt cgtcgagccg gtggaaatgt gcgaatcccg ccgtgtttgc gggatttgca
    10081 aggggcggtg ggaagggtgg aaaacctggt tttggttttc cacgctttcc acggacccgg
    10141 catttccacc ggcccccggc ctcgctaccg gaaccggggt ggaacccggg actgcgcttt
    10201 gcattgccgc aagattttcg ccttggcggc gctcatccgg cgcgcacact cggtgtcgct
    10261 catcgccagc gcctcccgct ccatctccgg caaactgact cccggcttca gacatttctc
    10321 gacttccggc agcgacttca gtttctcgaa cggcgtccgg tagtcctcgg tcctgtactt
    10381 gcgccgcctc ttgccccgct cgtccagact caccgtcgcg aagccgcacg gccggtggta
    10441 gttcagatac gggttgagat agtgcgcata aaacgtgccg atggcttcgg cgtgttcgcc
    10501 cgcgatgtag ccgtagccca tcagcttgcg gatcaccgct ccgttcttgc cttccaccag
    10561 cgcattgtcc tgactgcggc aggcgcggct tttggtgaac tccgcccgta gtttctcc
//
LOCUS       DASTMK010000032         7652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_225775, whole genome shotgun sequence.
ACCESSION   DASTMK010000032 DASTMK010000000
VERSION     DASTMK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225775"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1584)
                     /locus_tag="VFQ79_01200"
     CDS             complement(202..1584)
                     /locus_tag="VFQ79_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEU0138290.1"
                     /translation="MVRVYSSEEGPRLKTPMRLIALILLVCFSLCGQQASPRLVIISV
                     DGLMSRTLRNAESLGLRLPNLIEFRDRGTMSEGLVGVFPTVTYPTHTTMVTGRYPADH
                     GIVSNTLFDPERKLYGAWYWYSELIKVPTLWEAANAAGLKTGAVGWPVTAGAAIDFNV
                     PEYRAFRNEDDILLFRSLPTPGLLRAFEKARGAIKLDPGGHHDDLLSDMAAFVIETHK
                     PHLMLVHLIDLDHDQHLHGPESRPALRTLENIDRAIGKLRKAVEHAGLAKDTRWIIVS
                     DHGFFKVEKLFHPEAFLASMGLVGGADAGSWRVAVRNAGGCSAFVLKDPGDEEAKALV
                     LRMLGELKRDGGFGIDRIIERDELQRMHAWPDAFAALSMKEGWAAGSGRTGAWVTVSE
                     TTKGTHGYAPGPEALDATFMAFGPGIPARRIARGRMIDVAPTASRLLGLRMSELPGVD
                     LLASPASTKP"
     gene            complement(1602..1913)
                     /locus_tag="VFQ79_01205"
     CDS             complement(1602..1913)
                     /locus_tag="VFQ79_01205"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEU0138291.1"
                     /translation="MLLIRLLLAFSLLFAAACGTTSRQPEEQYLMRGEVVQLDTTNQV
                     ARINHEKIEGWMDAMTMEFPVKDKAEFDKLAVGDRITAIVYVRDLEYYIGKIEVVSKA
                     P"
     gene            complement(2129..3442)
                     /locus_tag="VFQ79_01210"
     CDS             complement(2129..3442)
                     /locus_tag="VFQ79_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU0138292.1"
                     /translation="MSHTTAPVLAANIVRTLRQSGYAAFLVGGCVRDLLLGIEPKDLD
                     VATNATPPEVLRLFPRAQPVGAHFGVILVPENGAKVEVATFRSDHAYQDGRRPGRVEF
                     EIDPKRDVLRRDFTINAMLMDPDTKEVLDFVGGRQDLESGIIRAIGEPEQRFREDHLR
                     MLRAIRFSARFNFAIEENTLRAMKSLAPLVRHVSAERVRDELTRMLTEGGARRAFELL
                     DDSGLLTQILPEVAAMKGVAQPPEFHPEGDVWTHTLMMLEGLREPTITLALGALLHDV
                     GKPPTFRVAERIRFDGHVEAGVEMTRRILSRLRFSNDEIRQVEALVAHHMRFKDVPQM
                     KDSTLKRFLRLEKFEEHLELHRLDCSASHRQLDNYFYAKEKLESLPAEELKPPRLLTG
                     DDLIEAGYTPGPQFAAMLTAVEDAQLEHRIHTKEEALELVKSLFR"
     gene            3506..5698
                     /locus_tag="VFQ79_01215"
     CDS             3506..5698
                     /locus_tag="VFQ79_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0138293.1"
                     /translation="MATTKVVVATTVALSFISFWRGAAIVLSDLASTMFYVGGIVERA
                     IGKPAPWFVIGVMLFSFAVRSVYMESCSMFVRGGVYVVVRDSLGPFMGKLSVSALVVD
                     YVLTGPISSVSAGQYLGRLFNELSGMAGMGLHIDPNDFATFFGVGVTGYFWWMNTKGI
                     HESSGKALRIMQITTVMVVILLIWCPITLLWRGDASLPPAPVPENLQFDSHSLGWLQG
                     TFWPQLAGVAIIIAFGHSLLAMSGFETLAQVYREIAYPKLKNLKITANIVCTYALLST
                     GLISLFAVMIIPDSIRPQYYDNLIGGLAMNLAGPELLKLVFHTFVVIVGGLILSGAVN
                     TAIIGANGVLNRVAEDGVLLSWFRKPHRRFGTTYRIINLIALLQVATILASRGDVYLL
                     GEAYAFGVVWSFFLKGVGVLALRFQRHDQEYKTPLNLRIRGVEIPIGLGLTTLLLFLV
                     AMANLLTKQVATVYGVSFTVVLFTLFIISERISKTRRTAGKQGLEEFNLDHQRDIESR
                     SLRARPGCILVAVRDYTRMDHFRRVLQKTNVRRHDIVVMTVRQMSAGAGEYGLTDQQL
                     FSDYEQELFSHVVEMAEKEGKTVELLVVPAEDPFDAMVQSAAALKASRLVTGVSAKMA
                     SEELARRIGLAWERLPEPRHAFSLEIISPDRASTYVNLGPHPPRLWPEDVDRLHELWL
                     KLGEEEGLGSKLHHRDVVGVALRLLNNTLSGPERDKVLEELRRELRRD"
     gene            complement(5695..6000)
                     /locus_tag="VFQ79_01220"
     CDS             complement(5695..6000)
                     /locus_tag="VFQ79_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138294.1"
                     /translation="MDEIRLGDVIDDHCVKCRRITNHSIVSIVDGKAAKVRCRTCYND
                     HDYRHEQAPPSKKDLKKMELFKEVLATVDPPSAEAQTPAPAEEESKGRKRGRRAGSA"
     gene            complement(6063..6581)
                     /locus_tag="VFQ79_01225"
     CDS             complement(6063..6581)
                     /locus_tag="VFQ79_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU0138295.1"
                     /translation="MNVFLLYLLLLKATIITFSGLASLPVIREDLVVHYGVLTDRELN
                     MAVAAGQLGPGPIGSYVVSAGYLAAGIPGAIAGWMAMITPAFLILPMLRYMGSRAKSP
                     GIRRMIRGVTIAAAGLILASAVPLAQSAISGPLTLAIAVSSFLALAFTRIDSIWIMAA
                     SAAAASISPMLV"
     gene            complement(6578..7120)
                     /locus_tag="VFQ79_01230"
     CDS             complement(6578..7120)
                     /locus_tag="VFQ79_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU0138296.1"
                     /translation="MPPRLTQLTAVFLRTGNTTFGGGEPTIAALQRELVTKRGWLGNE
                     QFGLAYGLARITPGTNLLAFCAAMAWFLRGWPGSVAAVLAVTVPSSALIVWVTHIDQI
                     GRSNPMAIAAIGGTLAAAVGMMVAGAWLLLWPHARRRTAHTAAIAGCAAFLLLKTGVA
                     PLVILAGAAAAGFFWRDGEE"
     gene            7191..>7652
                     /locus_tag="VFQ79_01235"
     CDS             7191..>7652
                     /locus_tag="VFQ79_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU0138297.1"
                     /translation="MRGKWILLAGIAILVAVIAGALSALWRDRVERARQAQEEAEAKK
                     VGPPASEISLSGTVQAQNVVPVPAPVNGTLEIYYVEEGQEVYEGQLLARIRNTRLETA
                     RDAASAELERIQTRVNNLESAIISGRLEASRASADANRARAEFEQLDKLYQR"
BASE COUNT         1543 a         2301 c         2256 g         1552 t
ORIGIN      
        1 gatcaccgtt tccggtacgc cttaccggga cctcggcgac gactactacg aaaggctcaa
       61 cagaaagcac ctggaaaggt cgctcctaaa gcggctccag aaactaggca atgaagtcac
      121 aatgaaaccg aaagcccaac cgctgcctgc acctacctga tactttcagg ggagcggtct
      181 ttcgcgtttt tcagcaagcc cctatggctt cgtggacgcc ggggaggcga gcagatccac
      241 gccgggtagt tcgctcatcc gcaatccgag caggcgggac gcggtcgggg cgacgtcgat
      301 catgcgcccg cgagcgattc ggcgagccgg tatgccgggg ccgaaagcca tgaacgtcgc
      361 gtcaagggct tcgggaccgg gtgcgtatcc gtgtgtcccc ttcgttgttt ccgacactgt
      421 gacccaggct ccggtgcggc cgcttccggc agcccaaccc tccttcatgc tcaacgccgc
      481 aaaggcgtcc ggccaggcgt gcatgcgctg tagctcatct ctctcgatga tccggtcaat
      541 tccaaagccg ccgtcccgtt taagttcacc gagcatccgc aggacgagcg ctttggcctc
      601 ctcgtcgccc gggtccttca gcacaaaggc agagcagccg cccgcattgc gcaccgcaac
      661 tcgccacgac cctgcgtccg cgccgccaac gagtcccatg ctggcgagga aggcctcggg
      721 atgaaacagc ttttcgacct tgaaaaaccc atggtcgctg acgatgatcc accgggtgtc
      781 tttcgcaaga cccgcatgct ccaccgcctt ccgcaatttg ccgatggccc ggtcgatgtt
      841 ctcgagcgtc cgcaatgcag gtctgctttc cgggccatga aggtgctgat cgtgatcgag
      901 gtcaatcagg tgcaccagca tcagatgcgg cttgtgcgtc tcgatcacaa atgcggccat
      961 atccgacagg aggtcgtcgt ggtgcccacc cgggtcgagc ttgattgcgc cacgagcttt
     1021 ctcaaaggcg cgcaatagtc cgggagtcgg aagggagcgg aacagcagga tatcgtcctc
     1081 gttgcggaaa gcgcgatatt cggggacgtt gaagtcgatg gccgcccccg ccgtcaccgg
     1141 ccagccgacg gcgcccgtct tcaatccggc ggcattcgcg gcttcccaga gtgttggaac
     1201 tttgatcaac tcggagtacc aataccacgc gccgtagagc tttcgctccg ggtcgaacag
     1261 ggtgtttgag acgatgccgt gatcggcagg gtagcgccct gtcaccatcg ttgtatgggt
     1321 tgggtaggtc accgtcggaa agacgccaac caagccttcg gacatggtgc cacggtcgcg
     1381 aaactcgatc agattcggca ggcgcagccc aagtgattcg gcgttgcgca gagtccgcga
     1441 catcaggccg tcgacgctga tgattacgag ccggggagac gcctgctggc cacacaggct
     1501 gaaacaaacc aatagtatca gggcgatgag gcgcatggga gttttgaggc gtggtccttc
     1561 ctcactggag tatacacgga ccaagatgac ttcctgatga attaaggcgc cttcgagact
     1621 acctcgatct tgccgatgta atactccagg tcgcggacgt agacgatagc cgtgatccgg
     1681 tctcccaccg ccagcttgtc gaactcggct ttatctttga ccgggaattc cattgtcatc
     1741 gcatccatcc agccctcaat cttctcgtgg ttgattcggg ctacctggtt tgtggtatcc
     1801 agttggacta cttcaccgcg catcagatac tgttcttccg gctgccgcga ggtggttccg
     1861 caggcggcgg caaacagaag agagaaggcc aacagaagtc gaatcagcaa catacgcaca
     1921 gtctagcact ggcattgaca ccggtctcaa cgaggcgcac aatgagggca acagaatcgg
     1981 gaggaggttt ccgatggcga gggcgaagat gcaaagacac gatacggatg agcggcgagt
     2041 catctacctc gagttcgtgg ccgcttcctc cattttcttc atcgttgcaa tggtcctcta
     2101 catggtattc agctaccggc cgacgtagct atctgaaaag cgacttcacc agttcgagcg
     2161 cttcttcctt cgtgtgaatc cggtgctcca actgggcgtc ttccaccgcc gtcagcatcg
     2221 ccgcgaattg gggccccggt gtgtagccag cctcgattaa atcgtccccg gtaaggagcc
     2281 gcggcggctt caactcctcg gctggcaggg attcgagctt ctccttcgca taaaagtaat
     2341 tgtccagttg gcggtggctc gcggaacaat cgaggcggtg caactccagg tgctcctcga
     2401 acttttccag gcgcaggaat cgcttcaacg tgctgtcctt catttggggc acgtccttaa
     2461 atcgcatatg atgcgccacc agcgcttcca cctgccggat ttcgtcgttc gaaaagcgca
     2521 ggcgcgaaag aattcttcgg gtcatctcca ccccggcttc cacgtggccg tcaaaacgaa
     2581 tacgttctgc cacccggaac gtgggcggct tgccgacatc gtgcagcagc gcgcccaaag
     2641 ccagagtgat ggtgggttcc cgaagccctt ccagcatcat taacgtgtgc gtccacacat
     2701 cgccttcggg gtgaaattcc ggcggctgcg ccactccttt catcgcggcc acctccggga
     2761 ggatctgcgt cagcaggccg gagtcgtcca gcagttcaaa ggcgcggcgc gctccgcctt
     2821 cggtcagcat ccgggtcagt tcgtcgcgca ccctttcggc tgatacgtgc cggacgagcg
     2881 gagcgaggct cttcatcgcg cgcagagtgt tctcttcgat agcgaagttg aatcgcgccg
     2941 agaaccggat ggcgcgcaac atgcggaggt gatcttcccg gaagcgctgt tcgggttctc
     3001 cgatggcgcg gataatgccg gattccaggt cctgcctgcc gccgacgaaa tccaggactt
     3061 ccttcgtatc ggggtccatc aacatcgcgt tgatggtgaa gtcccggcgg agcacatcgc
     3121 gtttgggatc tatctcgaat tcgactcgcc ccgggcgcct accgtcctga tatgcatgat
     3181 cgctgcggaa ggtggcaacc tccaccttgg caccgttttc gggaaccaat atcacgccga
     3241 aatgggcgcc caccggttgc gcccgcggga agagccgcag cacttcgggc ggcgtcgcat
     3301 tcgtcgctac gtccaaatcc ttcggttcga tgccgagcag caagtcacga acacagccac
     3361 ctaccaggaa agccgcgtat ccgctctgcc gcagagtccg gacgatgttt gctgcaagaa
     3421 cgggtgcggt ggtgtggctc attactttaa tcgtagaata gagccggtaa cacctctcct
     3481 aatgggctca cagccagacc ggtctatggc cacgacgaaa gtcgtcgtgg caacgacggt
     3541 tgcattatca tttatctcct tctggcgcgg cgccgccatc gtcctcagcg atctggcctc
     3601 cactatgttc tacgtcggag gcatcgtcga gcgcgccatc gggaagcccg caccctggtt
     3661 tgtaatcggc gtcatgctgt tcagcttcgc tgtgcgttcg gtttacatgg aaagctgcag
     3721 catgttcgtc cgcggcggcg tgtacgtagt ggtgcgggac agccttggcc ccttcatggg
     3781 caagctttcc gtctcggccc tagtggtgga ttacgtcctt accggaccca tcagctccgt
     3841 tagtgcaggg cagtatctgg gccgtctttt caatgaactg tccggcatgg cgggcatggg
     3901 gctgcatatc gacccgaatg acttcgccac gttcttcggc gtcggcgtaa ccggctactt
     3961 ctggtggatg aataccaagg gcatccacga atcgagcggc aaggccctgc gaatcatgca
     4021 gatcaccacc gtaatggtgg tcattctgct catctggtgc cctatcacgc ttctgtggcg
     4081 cggcgatgcc agcctgccac cggcgcccgt gccggaaaat ctccagtttg acagccattc
     4141 gctggggtgg cttcaaggaa cgttctggcc gcaactggcc ggcgtggcaa tcatcatcgc
     4201 gtttggccat tcgctgctcg caatgagcgg atttgagacg ctggcgcagg tatatcgcga
     4261 gatcgcttat cccaagctca agaacctgaa aatcactgcg aatatcgtct gcacctacgc
     4321 gctgctctcc accggattga tctcgctgtt cgcggtgatg attattccgg attcgatcag
     4381 accgcaatac tacgacaacc tcattggcgg actcgccatg aacctggctg gcccggaact
     4441 gttgaaactg gtctttcaca ccttcgtggt catcgtcggc ggcctcattc tttcgggtgc
     4501 cgtgaacacg gccataattg gcgcgaacgg agttctcaac cgcgttgccg aagacggtgt
     4561 gctgctgagc tggtttcgca agcctcaccg ccgcttcggc accacttacc gcatcatcaa
     4621 cctcatcgcg ttgttgcagg tggcaaccat tctggccagc cgcggagatg tgtatctgct
     4681 tggtgaggcg tacgccttcg gcgtggtttg gagctttttc ctgaagggcg tgggcgttct
     4741 cgcactccgc ttccaacgcc acgaccagga atataagacg ccgctcaacc ttcggattcg
     4801 gggggtggag atcccaatag gcttggggtt gacgaccttg ctgctcttcc tggtcgcgat
     4861 ggcgaacttg ctcaccaagc aggttgccac ggtctacgga gtttcattta cggtggtgtt
     4921 gttcaccctg ttcataatct ccgaacgcat cagcaaaacc cgccgaacgg ccggcaagca
     4981 gggcctggag gagtttaatc tcgaccatca gcgggacatc gaatcgcgaa gccttcgcgc
     5041 gcgccccggt tgcattctgg tggcggttcg cgattacacg cgcatggatc atttcaggcg
     5101 tgtactccag aagacgaatg tgcggcgcca cgatatcgtt gtcatgacgg tacgccagat
     5161 gtcggcgggc gccggggaat atgggctgac cgaccagcaa ctgttcagcg actacgaaca
     5221 ggagttattc agccacgtcg tcgaaatggc cgagaaggag ggcaagacgg ttgaacttct
     5281 ggtggtgccg gccgaggacc cgttcgatgc gatggtgcaa tcggcggcgg cactgaaggc
     5341 cagtcgcctg gtgacgggcg tatccgccaa gatggcttcg gaggaacttg cgcgccggat
     5401 cggtctggca tgggagcggc ttcccgaacc ccgccatgcg ttttcactgg agatcatcag
     5461 ccccgaccgg gcttccacgt atgtgaacct ggggccgcat ccaccccggc tatggcccga
     5521 agacgtggac cggctgcacg aactctggct gaaactgggc gaggaggaag ggcttggttc
     5581 gaaattacat cacagggatg ttgtcggtgt ggcgttgcgg cttctcaata ataccctcag
     5641 cgggcccgag cgggacaagg tgctggaaga gctgcggcgc gagctgagac gggattaagc
     5701 agatccggct ctccgtcccc gcttgcggcc cttcgattcc tcctccgcag gcgctggagt
     5761 ttgcgcttca gcggatgggg gatccaccgt ggcgaggacc tctttgaaga gttccatctt
     5821 cttcaggtct ttcttggagg gcggcgcttg ttcgtgacgg tagtcgtgat cgttatagca
     5881 ggttctgcaa cgtacttttg ccgcttttcc atcgacaatc gacacaatcg agtggtttgt
     5941 aatgcggcgg cacttgacgc aatgatcatc gatgacatca ccaaggcgaa tttcgtccat
     6001 ggagggctcc gctggaaatt aagttgcagt ataacagaaa atcacagcga ctaacccgca
     6061 gttcagacca gcattggact gatagatgcc gccgcggccg aagctgccat aatccagatg
     6121 gaatcgattc tcgtgaaggc cagagccagg aaactcgaaa cggcgatggc cagagtgagc
     6181 gggccgctta tagcgctctg ggccagcggc accgccgacg ccagaatcaa tccggcggcg
     6241 gcgatcgtaa cgcctcgaat catccgccgt ataccgggac ttttcgctcg cgaacccatg
     6301 taacgcagca tcggaaggat gagaaatgcg ggcgttatca tcgccatcca tccggcgatg
     6361 gcgcccggaa tgcctgccgc gagatacccg gcgctcacca cgtaagaacc gatcggcccc
     6421 ggccccagtt gccccgccgc cactgccata ttcagttcac gatcggtcag cacgccataa
     6481 tgcaccacaa gatcttcgcg aatgaccggc agcgaggcga ggccgctaaa agtaataatg
     6541 gtcgccttta acagaagcag gtacagcaaa aatacgttca ttcctctccg tcccgccaga
     6601 aaaaaccggc ggccgccgcc cccgccagaa tcacaagcgg cgcgactcca gttttcagta
     6661 aaagaaatgc cgcgcaaccg gcaatggcgg cggtatgcgc tgttcgccgg cgggcgtgcg
     6721 gccacaacag gagccaggct cccgccacca tcattcccac cgcggcggca agcgtcccgc
     6781 cgatcgccgc aatcgccatg gggttcgacc tgccgatttg gtcgatatgg gtaacccaga
     6841 cgatcaatgc agaggagggg accgtcactg ccagtacagc cgcgacagag ccgggccaac
     6901 cgcgcaggaa ccacgccatg gctgcgcaga aagccagcag attcgtgccg ggtgtgatcc
     6961 gcgccaggcc atatgccagc ccgaactgct cattgcccag ccagccacgc ttcgtaacca
     7021 gctcgcgctg taacgcggca atggtgggtt caccaccgcc aaacgtcgtg ttgccggtgc
     7081 gcaggaaaac ggcagtgagt tgtgttaacc gtggcggcat cttcctcaat ttacgccata
     7141 cacgacgcct gcacaggtgc ctggcggcat gccctgagta tgattgaagt atgcgcggga
     7201 aatggatctt gctcgccgga atcgccatcc tggtggcggt aatcgctggt gctctttccg
     7261 cgttgtggcg cgatcgggtc gagagggccc ggcaggcaca ggaagaggcc gaggctaaaa
     7321 aagtgggccc tccggccagt gaaatcagcc tttccggaac agtccaggcg cagaatgttg
     7381 tacccgtgcc tgctcctgtg aatggcaccc tggaaattta ttacgtagag gaaggccagg
     7441 aggtttacga aggacaactg ctggccagaa tacggaacac acggcttgaa acagcacggg
     7501 atgcggcctc ggcagaattg gagcgcattc aaacgcgcgt caataacctg gaaagcgcca
     7561 tcatctccgg ccgcctggag gcttcccggg cctccgccga cgcgaaccgc gcccgcgcgg
     7621 agttcgaaca gttggataag ctgtaccagc gc
//
LOCUS       DASTMK010000033         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_130078, whole genome shotgun sequence.
ACCESSION   DASTMK010000033 DASTMK010000000
VERSION     DASTMK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130078"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..792
                     /locus_tag="VFQ79_01240"
     CDS             172..792
                     /locus_tag="VFQ79_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138298.1"
                     /translation="MRLAMGSYNPAVIFGQLPPFTIAGLLPPYVGGDPTAAHLMAPYP
                     TTIDLLVRHFATSPRRREILQGFLGFRQALASAGICDGFHWLNGSFSENVELRENRDP
                     GDLDLVTFFRRPIVAKDDATFAGFFAANRNLFTPAYSKQQFSCDTYFVDLDTDSVSVV
                     AQTRYWYGLFSHRRVTGEWKGMIELPVALSQADVDAANMLGQQVAP"
     gene            789..1280
                     /locus_tag="VFQ79_01245"
     CDS             789..1280
                     /locus_tag="VFQ79_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138299.1"
                     /translation="MTRRLERAQLLAEVASLEAMLKTILPNDLVGRLGFESRLEEARG
                     KLAQLDEHEERLASVALYFGGEPVFGSRGIRAEFGSTIIASYQDLIAKVWATLAGGNL
                     SLRGQIRDKDAAQLHITNLVHGSFGFRSSQTSRRSRHTRFPTQGPSQGDRQTSSEPGG
                     LRY"
     gene            complement(1319..2065)
                     /locus_tag="VFQ79_01250"
     CDS             complement(1319..2065)
                     /locus_tag="VFQ79_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138300.1"
                     /translation="MTDKTLFLAWQDKRLTRAWFPVGRLDVRSTPRRYRFRYTQGAER
                     AQAEAGFEPLFDFPEWKRNYLSADLFPLFQNRVISSERKDFSDYLRMLDLPQTAEPAE
                     ILEVGGGYRATDNFEVFPKIERRPDGSFRCRFFLHGMRHVAPAALERLDRLTPGEQLY
                     VTLELTNPATTLAVQLQTTDYHMIGWAPRYLVDDLMRAMAHAPQGYAAKVVRLNPSPA
                     PSKQRLLVELSGSWPDYEPMATGDFEPLIQ"
     gene            complement(2062..2463)
                     /locus_tag="VFQ79_01255"
     CDS             complement(2062..2463)
                     /locus_tag="VFQ79_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138301.1"
                     /translation="MKRTPTGRAGIIAPSFDHASSLGRELLDDTSGKCRLRILKEKRI
                     GQYAESAPGAIFWEESDARAISPLELARRATASYPDLFRPALAGLANLKKESLERIAG
                     RVPTPWMSGAARQFAIELTCYNLKQLREIVS"
     gene            2482..3147
                     /locus_tag="VFQ79_01260"
     CDS             2482..3147
                     /locus_tag="VFQ79_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138302.1"
                     /translation="MMPVRIADQRVENQILRDSLLSRCRRLGDSDQMLARLLVEYGPA
                     EISRMLGVSRSTVYARIARLRSPNPSPGGVMDVEAVLDRLMEAGVSVWLDIEGKLLID
                     KGAPEEIKQIVREHKQELIDVRRAQAIINRPRMRCIRLPLGHLAVAYPPGADLEEIRW
                     AMRVLRMDSMPLVINDEGLEWISPEEWRRRQVARIFAEQRREQLRKATVEAERTKERR
                     RQA"
     gene            3144..3431
                     /locus_tag="VFQ79_01265"
     CDS             3144..3431
                     /locus_tag="VFQ79_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEU0138303.1"
                     /translation="MTTERAIVKAILAYLNSLPVCLARKRWGGGMGVAGDPDIDACIR
                     GRSLQLEVKRPGEKPTALQVKRIDEWRRAGAIVAVVNSVAEVRDLLDKEFH"
     gene            3584..3928
                     /locus_tag="VFQ79_01270"
     CDS             3584..3928
                     /locus_tag="VFQ79_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138304.1"
                     /translation="MRKTIRIENPVSDCRFTSKNRAKRFVAQGRAEWAEPGISIRFIQ
                     SDHRHASAQRAADVTRFWYERASNTGMATISEIANLPMVMPARAMGFGRFRGANRHTF
                     LSQEPKSCTSAA"
BASE COUNT          826 a         1222 c         1226 g          794 t
ORIGIN      
        1 ccggcggtca cctgccactc ggctacgatc tggatgacgg caacctcgtc gtcaacgagg
       61 aggaggccgc gcgcgtacgc cagatcttcg agtggtacct cgagggccac agcgtcttca
      121 gcatcgtggc caattgcgag acggcacgcg tgcgagcgtc acacggtcga attgcgctta
      181 gccatgggca gctacaaccc ggcagtcatc ttcggccagt taccgccatt cacaattgca
      241 gggctgctgc ctccgtacgt cggaggggat cccactgccg cccacttgat ggctccctac
      301 ccgacaacaa tcgatctttt ggttcgtcat tttgcgactt ctccacgcag gcgtgagatc
      361 ctccagggtt ttctgggatt tcggcaggcg ctcgcctccg caggtatctg cgatggcttc
      421 cattggctga atggcagttt ttcagaaaac gtcgaacttc gggaaaatcg agatccgggt
      481 gacctggatc tcgtaacttt ctttcgccga ccaatcgtcg cgaaagacga cgccacgttc
      541 gccggctttt tcgcggctaa ccgcaaccta ttcacgccgg cgtattccaa gcagcaattc
      601 tcgtgtgata cctactttgt cgatctggat accgattccg ttagcgtagt cgcgcagacg
      661 cgctattggt acggcctctt ttcgcaccgg cgggttaccg gcgagtggaa ggggatgatc
      721 gaattgcccg tggcgctatc gcaggccgac gtcgatgcag caaacatgct tggacagcag
      781 gtagcgccat gacgcgtaga ctagagcgcg ctcaactgct ggccgaggtc gcctctctcg
      841 aggcaatgct gaagacgatc ctaccgaatg acctggtcgg tcgtttgggc ttcgaatcgc
      901 gactggagga agcacgcggc aaactcgccc aacttgacga acacgaggag cgcctggcgt
      961 ccgtagcttt atatttcggc ggcgaaccgg tattcggcag tcgcggaatc cgcgccgagt
     1021 tcgggtcgac tatcattgca agctaccagg acctgattgc aaaagtctgg gccaccctcg
     1081 ccgggggcaa tctctcgctc cggggacaga tccgagacaa agatgccgcg caactccaca
     1141 tcacgaatct tgtgcatgga tcgttcgggt tccgttcgag ccaaacgtct cgtcgttcca
     1201 ggcatacacg ctttcccacc caaggacctt ctcaaggtga tcggcaaacg tcttctgaac
     1261 caggcggtct tcgctattga tgttcgtgta ggccatggtt tcggcgcatg gcgctgcact
     1321 actgaatcag gggctcgaaa tcaccggtgg ccatcggttc gtagtccggc cagcttccgg
     1381 ataattccac cagcagacgt tgtttggacg gggcgggcga aggattcaac cgtacgacct
     1441 tggcggcata gccctgtggc gcatgcgcca tggcccgcat cagatcgtcc acaaggtacc
     1501 gcggcgccca accgatcatg tggtagtcag tggtttgcag ttgtaccgcc agcgtggtgg
     1561 ccgggttggt caactccagc gttacgtaga gttgctcccc aggcgtcaaa cgatcaaggc
     1621 gctccaacgc cgccggcgca acatgccgca tcccgtgcag gaagaagcgg caacggaacg
     1681 aaccgtcggg gcggcgctcg atcttcggaa aaacctcaaa gttgtccgtg gcgcgatacc
     1741 cgcctccaac ctcaaggatc tcggctggtt cggcggtctg aggcagatcg agcatccgta
     1801 ggtaatcgct aaaatcttta cgttccgagg agatcacccg gttctggaac aacgggaaca
     1861 aatcggcgga aagatagttt cgcttccact ccgggaaatc gaacagaggc tcaaaaccgg
     1921 cctcggcctg ggcgcgttcc gcgccctggg tataccggaa ccggtaacgg cgaggcgtgc
     1981 tgcggacatc caggcggcca acgggaaacc aggctcgcgt cagccgtttg tcctgccacg
     2041 ccaaaaacag ggttttgtcc gtcatgacac gatctctctc aattgtttca ggttgtagca
     2101 cgttaactct atcgcgaatt ggcgcgccgc gccactcatc cagggtgtgg gcacacgccc
     2161 ggcgatgcgc tccaggctct ccttctttag attagcaagt cccgcaagtg cgggtcggaa
     2221 caggtccggg tagcttgcag tggcgcgtcg cgccagttcc agcggactga tggcgcgcgc
     2281 atcgctctcc tcccaaaata tggcgcccgg ggcgctctcc gcatactgtc caatccgctt
     2341 ctccttgaga atccggaggc ggcactttcc cgaggtgtcg tcgagcaact cgcgtcccag
     2401 cgaggaggca tgatcgaagg acggcgcgat gattccggcc cggcccgttg gcgtgcgctt
     2461 caccagaatg ccccagttct catgatgccg gtccgtattg ccgatcagcg cgtcgagaac
     2521 cagatactgc gcgattcgtt gcttagccgc tgtcgccgcc tcggcgattc ggaccagatg
     2581 ctggcacgcc tgctggtcga atacggccct gccgagatca gccggatgct tggcgtctcc
     2641 cgctccaccg tgtacgcccg cattgcgcgg ctccgctcac ccaatccgtc accgggaggg
     2701 gtcatggacg ttgaggctgt tctggaccgg ctgatggaag ctggcgtatc tgtctggctg
     2761 gacatcgagg gcaagctgct tatcgacaaa ggcgcgccgg aagaaatcaa gcagatcgtc
     2821 cgtgagcaca agcaggagtt gatcgacgtg cggcgagccc aggccatcat taaccgtccg
     2881 agaatgcggt gtatccggtt gccgttgggc cacctcgcgg tggcttaccc gccgggcgcc
     2941 gatctcgaag agatccgttg ggcaatgcgc gtgctccgca tggattcgat gccactggtc
     3001 atcaacgatg aggggctgga atggatcagc ccggaggagt ggcgccgccg gcaggtagcc
     3061 aggatcttcg ccgaacaacg gcgagaacaa cttaggaagg caaccgttga agcggagcgg
     3121 accaaagaac ggaggcggca ggcatgacga cggaacgggc gatcgtgaaa gcgattctgg
     3181 cgtatctcaa cagccttcct gtttgcctgg cgcgcaagcg ttggggcggc ggcatgggtg
     3241 tggcgggaga ccccgacatc gacgcctgca tccgcggccg cagcctccaa ctcgaggtca
     3301 agcgacccgg agaaaagccg acggcgctgc aggtgaagcg aatcgatgaa tggcgaagag
     3361 ccggcgcgat tgtggccgtg gttaacagcg tcgccgaagt tcgcgacttg ctcgacaagg
     3421 aatttcatta ggccgccgga cagatcagcc caaaccggcg tatatagtca tcgagaagcc
     3481 gttctgttgg caagcgcgaa gcgcaacctt ccgaaggatt ccttccttgt cgcagttccc
     3541 ttgcgggcat acccgcaatc ccctccgggc cgacaccaga actatgcgta aaaccattcg
     3601 aatcgaaaac ccggtctccg attgccggtt tacctcgaaa aaccgcgcga agcgcttcgt
     3661 cgcgcagggg cgcgcagagt gggcggagcc gggaatttcc attcgattca tccaatccga
     3721 tcaccggcac gcctctgcgc aaagggcggc agacgtcacg cgattttggt atgagcgggc
     3781 ctccaacacg gggatggcaa cgatttcaga gattgcgaac ctgcccatgg tcatgcctgc
     3841 cagagccatg ggattcggac gattcagggg tgccaacagg cacacgttcc tgtcgcagga
     3901 gcccaagagt tgcacttctg cggcatgaaa gtcttcaagc aaacaacgcc tcacggcttt
     3961 ccgtccaggg ttcgcatccc gcggtataaa gcacccgtcc ggatcctgat gaaggacggc
     4021 ctgccgtgcg acgaagtagt ggacgtgacc attcgcgcga gaacgata
//
LOCUS       DASTMK010000034         6238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_354418, whole genome shotgun sequence.
ACCESSION   DASTMK010000034 DASTMK010000000
VERSION     DASTMK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354418"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VFQ79_01275"
     CDS             <1..1877
                     /locus_tag="VFQ79_01275"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0138305.1"
                     /translation="ARVTGSFDRQGYRVEKIVYETQPGFHVSANLYIPTTTSAPFPGV
                     LFQMGHSSNGKAWDSYQRCCQGLVRLGFLVLAFDPMGQGERTYYPGPNPSRSRLPGGA
                     DDEHTVPGKQMLLIGDSSVRLQTWDAVRSLDYLASHPMVDPKRLASTGTSGGGTNTML
                     LAAVDDRLAAAAVACGNTENIACRNFNPPGATDDAEQNFAGSGPLGFDRWDLLYPMAP
                     KPLLFVPSANDFAGTYSPNYIASGLEEFAKLQRVYAALGHADRIAWEDSPLPHNLAYG
                     SRIAIYNWFLRWLKQEKPVNEEPEVAPEKDEVLFAAKGSTVLTLGSETPLTMLRKRPI
                     EKVSADLPHLLSLDDVEAAPPIRLAKTKFRRADIEAIEIRSASNVFLPAWLYQPRTGR
                     AKALLLLIDPAGRAQWQEGGLYETLAASGYSVCALDVRGIGHLTPEYSRGAARHTASH
                     HSEQHYSWSSMILGKPLLGQRVTDILATAGALRAAPGMPDRIVIAARGILTAPALFAA
                     ALDSKIHAVYLAGGLVSYRNVLEMEEYRGGNYHNPAVANQQDLFGAFVPGLLKQTDLP
                     ELAASMAPRRLTLAGTIDAAGKPLDIETVRKVYAAAANVQVTANAAWDATALSAAAGS
                     "
     gene            complement(1881..3446)
                     /locus_tag="VFQ79_01280"
     CDS             complement(1881..3446)
                     /locus_tag="VFQ79_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0138306.1"
                     /translation="MNDDCTISGLLAGADSQRLAIADVRGYGLTYGRLRSQIEQTHEA
                     LRKGGLGPDDTVGVVLRNGPETAAVLLALTSFCRVAPLNPNYTAAEFDFALHDLNAAA
                     VVTTEDIPAATAAACRCELGLILLKAAKSRVPAEYKLEIRRPLARTECAWSAPEPDNI
                     ALLLHTSGTTSRPKLVPLTQRNLCLSSNSVARVLQLAPEDRSLTVMPLFHIHGIVAGL
                     LAPVAAGSAVCCAPGFQATRFFSWIDAARPTWYTAVPTMHQAVLARARHNAATLDWHC
                     LRLIRSSSSPLYSSLWDELEATFGVPVLNAYGMTEAAHQIASVRLPGGPRYRNTVGPS
                     SGPEIAVLDSRSVPVSNGGSGEIALRGEQITRGYLQPPEASSAFAGGWFRTGDEGRID
                     GNGLLTLTGRLKEIINSGGEKVSPYEVEEALLTHPGVAEAAVFAAPHRMLGEEVASAI
                     VAREGFHIAERDLLRTAGLRLAPHKMPRRVLFVDSIPRGATGKIQRAGLAAQLGLADS
                     PAREHALDEHTRT"
     gene            3550..4452
                     /locus_tag="VFQ79_01285"
     CDS             3550..4452
                     /locus_tag="VFQ79_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU0138307.1"
                     /translation="MRLSEIVRFADVRVLRDGDFENLGFLTDTQPCSLVFLESERFAA
                     ALRRNKDVSAVLSAPAVADLVPEHMALAVCPEPRLTFAVVHNGLAQSGFYWEGFETVI
                     DPTAQVHPMASVAERNVRIACGCSIGPHATIGERCVLGEHVTIGAGAVLGAVGFQTVR
                     TNAQTLEMRHAGGLRVHERVHVLPGAVIATGLFRKNTEISSDARIGSQAFVSHGVHVG
                     ERAFVGHGAIVNGGVSIGREAWVGPGAVVAHSLDIGPQAFVSLGAAVIRSVKAGSHVS
                     GNFAVSHRRLLRLLTELDPQAAGR"
     gene            4449..5630
                     /locus_tag="VFQ79_01290"
     CDS             4449..5630
                     /locus_tag="VFQ79_01290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0138308.1"
                     /translation="MRVLLTNNGFGLRAGSELYVRDVAIELMQRGHCPIAYSTRLGSV
                     ADELRAATVPVIRSLESLGEPPDIIHGHHHYETLTAMMWFPQTPAISYCHGWTPWEEA
                     PLRFPRILRYVAVDELCRERLIVEGGIPAERIELILNFFDKRRFPRRTPLPSSPRLAL
                     AFSNEFSDRNDLPKLREACAQCGIELHAIGKGAGASEPDPGRLLTRYDVVFAKARSAI
                     EAMAAGTAVVLCAPGRMGTMVSSENFALFRRSNFGIRTLSRPIEVDSLVAELRKYDPA
                     DAATVSQAARDNCELQTAVDQILDLYKRVIDEARSMPLISSAEAERAGARYLEQSAPH
                     YKANSDERLNAEQRSGALQDEISALRSSATWRWTQGILQNRLVQWLFGPIIRSVAERS
                     N"
     gene            complement(5678..>6238)
                     /locus_tag="VFQ79_01295"
     CDS             complement(5678..>6238)
                     /locus_tag="VFQ79_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138309.1"
                     /translation="AALEEPVRGGAFPPNSTLGKTPETARCRVPDEFRTKMSGGWFRT
                     TVGGGRLLWRPERITEKGMGAEVAGEVRSPASAPPRIKGLPSAWRLRRYRDGKRILIH
                     AIPGEVETVLHATLRNHFTREPVIEKLRFPALQNALEVESGNFASVRLHSPDLAESRA
                     AKRSGNGWTVEAAGVKRYFVIECTEI"
BASE COUNT         1183 a         1960 c         1911 g         1184 t
ORIGIN      
        1 atgcacgcgt caccggatcg tttgaccgcc agggttaccg ggtggagaag atcgtgtatg
       61 aaacacagcc ggggttccac gtttcggcaa atctctacat tcccaccact acaagtgctc
      121 ccttccctgg cgtgttattc cagatgggcc attcctccaa cggcaaagcg tgggattcct
      181 accagagatg ctgccagggt cttgtacggc tgggctttct ggtgctggct ttcgatccga
      241 tgggtcaggg cgagcgcacc tactaccccg gacccaaccc gtcgcgcagc cgtcttcccg
      301 gaggggccga cgacgagcac acggtaccgg gcaagcagat gttgctgatc ggtgattcga
      361 gcgtgcggct tcaaacctgg gatgcggtgc gcagtctcga ctatcttgcg tcgcacccga
      421 tggtggatcc caagcggctg gcgtccacgg gaacatccgg aggcggcacc aatacgatgc
      481 tgctcgcagc cgttgacgac cgcctggcgg cagccgccgt cgcctgcggc aacaccgaga
      541 acatcgcctg ccggaacttc aaccctccag gcgccaccga cgacgccgaa cagaacttcg
      601 ccggctcggg accgctgggc ttcgatcgct gggacctgct ttacccgatg gcgcccaagc
      661 ctttgttgtt tgtcccgagc gcgaacgatt ttgcaggcac ttactcgccc aattacatcg
      721 cgagtggttt ggaggagttc gccaaacttc aaagagtcta tgccgcctta ggccacgcgg
      781 accgtattgc gtgggaagat tccccactgc cacataacct cgcttacgga tcgcgcattg
      841 cgatttacaa ctggtttctg cgctggctca agcaggagaa accggtgaac gaagaacccg
      901 aggtggcgcc ggagaaggac gaagtgttgt tcgccgccaa gggcagcacg gttcttactc
      961 ttggcagcga gacgccgctg acgatgctgc gcaaacgccc catcgaaaaa gtttccgcgg
     1021 accttccaca cctgctgtct ctcgacgacg tggaagctgc cccgcccatc cggctggcga
     1081 aaacaaagtt ccggcgcgcg gacatcgagg cgatcgagat ccgttcggca tcgaacgttt
     1141 ttctcccggc atggttgtac cagccaagaa ccggccgcgc aaaagcgctg ttgctgctca
     1201 tcgatccggc aggccgtgcg caatggcagg aaggcgggct ctacgaaact ctggccgcga
     1261 gcggctattc ggtttgcgcc ctcgacgtgc gcggtatcgg ccatctcacg cccgaataca
     1321 gccgcggcgc ggcacggcat acggcgtcac atcattccga acagcactac tcctggtctt
     1381 ccatgattct gggcaagccg ctgctgggcc agcgcgtcac cgacattctc gcaacggccg
     1441 gggcgttgcg cgccgccccg ggtatgcctg atcgaatcgt gatcgccgcg cgcggcattc
     1501 tgacggcgcc cgccctgttt gccgcggcgc tcgacagcaa gatccacgct gtatacctgg
     1561 caggtggatt ggtctcctac cgtaatgttc tggagatgga ggagtatcgc ggcggcaatt
     1621 accacaatcc ggccgtggcc aaccagcagg acctcttcgg cgcgtttgtt cccggcttgc
     1681 tgaaacagac agacctgcct gagctggccg cgtccatggc gccgcgccgc ctcacgcttg
     1741 ccggaacgat cgatgccgcc ggcaagcctc tggacatcga aaccgtcagg aaggtgtacg
     1801 cggctgccgc caacgtgcaa gtcaccgcaa acgcggcatg ggacgccact gctctcagtg
     1861 ccgcagcggg atcgtagcct tcacgtccgg gtgtgctcat cgagagcgtg ctcacgcgca
     1921 ggtgaatcag caagccccag ttgcgcggca agaccggctc tttgaatctt ccccgtggcg
     1981 ccgcgaggga tagagtcgac aaataggacg cggcgcggca tcttgtgagg cgccagtcgc
     2041 aggcccgccg ttcgtagcag gtcccgttcg gcgatgtgga acccttcgcg ggcaacgatc
     2101 gccgaggcga cctcttcgcc gagcatccgg tgcggtgcgg cgaaaacagc cgcttcggca
     2161 acaccagggt gggtcagcag cgcctcttcc acctcgtaag gagacacctt ttctcctccg
     2221 ctgttgatga tttccttcaa ccgtccggtg agcgtcagca gcccatttcc gtcaatccgt
     2281 ccttcgtcgc ccgtacgaaa ccagccgccg gcgaaggccg agctcgcttc cggcggctga
     2341 aggtaacccc gggtgatctg ttctcctcgc agcgcaatct cgccagaccc gccgttcgag
     2401 accggaacgc tccgcgagtc gaggacggca atttccggtc ccgaggaagg acctacggta
     2461 ttgcgatagc gagggccgcc gggcagcctg acactggcga tctgatgcgc cgcttcggtc
     2521 atcccatagg cgttcaggac cggcactcca aacgtcgctt cgagttcgtc ccacaggctt
     2581 gagtacaacg gagaggacga cgagcggatc aaccgcaggc agtgccagtc cagtgtcgcg
     2641 gcgttgtggc gggcgcgcgc caacaccgcc tggtgcatcg tgggaacagc cgtgtaccag
     2701 gtcggcctgg cggcgtcgat ccaggaaaag aacctggtcg cctgaaaacc aggagcgcag
     2761 caaacggcgc tgcccgccgc taccggcgcg aggagacccg cgacaattcc gtgaatatgg
     2821 aatagcggca tgaccgtgag cgaccggtct tccggagcaa gctgaagcac tcgcgcgacg
     2881 ctattcgatg acaggcataa gttacgctga gtgaggggaa ccagtttcgg ccgggatgtt
     2941 gttcccgaag tgtgcaggag cagggcaatg ttgtccggtt ccggggcgga ccaggcgcat
     3001 tccgtcctcg ccaggggccg ccggatttcc aacttgtatt cggcgggaac gcgtgatttc
     3061 gccgccttca gcagtatcag ccccagttcg caacggcacg cggcagccgt ggctgccgga
     3121 atgtcctccg tagtaacaac agcggctgca ttcaaatcgt gcaacgcaaa gtcgaattcc
     3181 gccgccgtgt aattcgggtt gagcggcgcc acacggcaaa acgacgtgag cgccagcaac
     3241 acggccgcgg tttccggacc gttccgaagc acaactccaa cggtgtcgtc cggtcccagg
     3301 ccgcccttcc tcagtgcttc gtgtgtttgc tcaatctgtg agcgcagtct tccatacgtc
     3361 agcccatacc ctcttacatc cgcgatcgcg agccgctgcg aatcggcgcc cgccaacaga
     3421 ccggaaattg tgcaatcgtc attcatagga taaaaacgtg gacgcactcc ctaactcagc
     3481 aaacaacagc atattacacc ggacccgcca agcaaccgcg atgcacaatc aactgggagg
     3541 gcagcttcca tgcgcctgag cgagatcgtt cgttttgcgg acgtgagagt gctgcgtgac
     3601 ggcgatttcg aaaatctggg gtttctcacg gacacccagc cgtgcagcct cgtattcctc
     3661 gagagcgaac gattcgcggc tgcactgcgg cgcaacaaag acgtctcagc agtgctgtcg
     3721 gcgcccgcgg tcgcggacct ggtgccagag cacatggcgc tagccgtatg tccagagccg
     3781 cggcttacct tcgcggttgt ccacaatggg ctggcccaga gcggcttcta ttgggaggga
     3841 ttcgaaacgg ttatcgatcc aacagcgcaa gtgcatccga tggcgtcggt ggcggagagg
     3901 aatgtacgta tcgcctgcgg atgttcgatt gggccgcacg caacaatcgg ggagcggtgc
     3961 gtgctgggtg aacatgtgac aatcggggcc ggggccgtcc ttggagccgt gggatttcag
     4021 accgtgcgaa caaatgccca gacattggaa atgcggcatg caggcggttt gagggtgcac
     4081 gagcgggtgc atgtgcttcc aggcgcagtg attgccaccg gactgttccg gaagaatacg
     4141 gagatatcct ccgatgcgag aatcggctca caggcattcg tgtcgcatgg cgtacatgtg
     4201 ggcgagcggg cgtttgttgg acatggagcc attgtgaacg gcggcgtctc gatcggccgg
     4261 gaagcctggg tcggtccggg cgcggtagtt gcgcacagtc tcgacattgg cccccaggcg
     4321 tttgtgagcc ttggcgcggc ggtgattcgc agcgtcaagg ccgggtcgca tgtgagcggc
     4381 aattttgccg tctcgcatcg ccggctcttg cggcttctta ctgaactgga tccgcaggcg
     4441 gcgggccgat gagagttctc cttaccaaca atggctttgg actacgggca ggcagcgagc
     4501 tttatgtgcg cgacgtggcg atcgaactga tgcaacgcgg ccattgcccg atcgcctata
     4561 gcacgcgtct gggaagcgtt gccgacgaac tgcgagcggc aaccgtgccg gtcattcgaa
     4621 gcctggaatc ccttggtgag ccgcccgaca tcattcatgg gcaccatcat tacgaaacgc
     4681 tgactgcgat gatgtggttt ccgcagacgc ccgccatctc ctattgtcac ggctggacgc
     4741 cgtgggaaga agcccccttg cgcttcccgc gcattctgcg ctacgtagcg gtggatgagt
     4801 tgtgccggga gcgattgatt gttgagggag gcatcccagc cgaacgaatc gagctgatcc
     4861 tgaatttttt cgacaaaaga cggtttcccc gtcgcacgcc tcttccgtcc tcaccacggc
     4921 tggcgctcgc tttcagtaac gaattctcgg atagaaacga ccttcccaaa ctgcgggaag
     4981 cctgcgccca atgcggaatc gaactccatg ctattggcaa gggggcaggc gcttcggagc
     5041 ccgacccagg ccgcttgttg acgcggtatg acgtcgtctt tgcgaaggcg cggtcggcca
     5101 tcgaggcaat ggccgccgga acggcagtgg tgctttgcgc tccaggacgg atgggaacga
     5161 tggtgtcatc ggagaacttt gcgttattcc ggcgctcgaa cttcggcatc aggacgcttt
     5221 cccgtccaat cgaggtagat tcgctggtag cggagctgcg gaaatacgat ccggccgacg
     5281 ccgcaacggt ctcccaggct gcacgcgata attgcgagct gcaaaccgcc gtcgatcaaa
     5341 tcctggatct ttataagcgg gtgatcgatg aggcccgctc gatgccgctg atctcgtcag
     5401 ccgaagccga acgtgcggga gcgcgctacc tggagcaatc ggcgccgcat tataaagcca
     5461 acagcgacga gcggctgaat gccgagcaaa ggtcgggcgc cctacaggac gagatttccg
     5521 ctcttcggtc ctcggcaaca tggcggtgga cgcaaggaat cctacagaac aggttggttc
     5581 agtggctatt cgggcccatc atccggtctg tcgctgaacg ttccaactga agccggagcc
     5641 ggccgcgtcg atggcgccgc ggcggcgttg taagatttca gatttccgtg cactcgatga
     5701 cgaagtaccg cttcacgcct gccgcctcta ccgtccagcc attcccgctg cgcttggccg
     5761 cccgcgattc cgcgagatcc gggctgtgta aacgaaccga ggcgaagttc cccgattcga
     5821 cctccagcgc attctgcaac gccgggaacc gcaacttctc aatcaccggt tcgcgcgtga
     5881 agtggttccg cagagtggcg tgaaggacgg tctccacttc gcccggtatg gcgtggatca
     5941 gtatacgctt tccgtcccgg taccgccgca gccgccacgc ggacggcagt cctttgatcc
     6001 gcggcggcgc gctcgccgga cttctcacct ctcctgccac ctccgcgccc atcccttttt
     6061 ccgtgatccg ttccgggcgc cacagcaggc gcccgccgcc caccgttgta cggaaccatc
     6121 cgccgctcat cttcgtccga aactcgtccg gcacgcggca ccgcgcggtc tccggcgtct
     6181 tccccaacgt actattgggc ggaaacgcgc cgcctcgaac cggttcctcc aaagccgc
//
LOCUS       DASTMK010000035        13151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_417921, whole genome shotgun sequence.
ACCESSION   DASTMK010000035 DASTMK010000000
VERSION     DASTMK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417921"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFQ79_01300"
     CDS             <1..509
                     /locus_tag="VFQ79_01300"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU0138310.1"
                     /translation="TDMPGQAVYRIDAPGKVVRLLAAPEIQRPNGIQVAPDDRTLYLI
                     EANGAEGGARMIRAYDLLPDGRVRNMRVHYNFYPGRSADGMSIDVDGNLYASAGLHRR
                     RGTSETLDTKCGVHVISPRGKLLKFIPVPEDTITNNAFGGPDGKTLYITAGKTLFKVR
                     VDRPGLPR"
     gene            636..809
                     /locus_tag="VFQ79_01305"
     CDS             636..809
                     /locus_tag="VFQ79_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138311.1"
                     /translation="MTRKIWMAAALALAMVGGTFAQTPPSQPVEKTAKTEKAKKHKKV
                     KKSDKKPSTSSTQ"
     gene            853..2571
                     /locus_tag="VFQ79_01310"
     CDS             853..2571
                     /locus_tag="VFQ79_01310"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEU0138312.1"
                     /translation="MRKCRVALPETDSPPPARSAAPSPIKRLATAVAIAVAGGSGILN
                     LLSLLGGPAPAENPRWLWKVLPFDFVGLSRSLTLLLGFILILTALHLSSGKRRALQLA
                     IGLAGTSGILHLIRMELPELLSSSAIIALLLWARDLFTAGSARPALLPAVRRALIALA
                     VPFLYGVGGFWLLEPHEFGKNFHWWDAIERTARLLAFLDDKTLLPKTAYAAWFLDSIT
                     LILLASFLYACLILFRPAAYHFRLNPVEQDRAAAIAEDYGRSAQDYFKHYPDKSYFFS
                     KSGRAFIAYRVAGNFAVALGDPVGPEHELPEIVEDFAGYCRERGWGVAFHQVFPDRLS
                     MYRALGFRRLKIGDDALVDLHSFSLSGSAAKEFRNTVNRLKRYGYRVERFDAPIDRER
                     MEELKNVSEAWLKLPGHRERRFTLGKFDRDYVRSTTVYAALDGHGHVAAFLNLVPSYC
                     RGLATVDLMRRHPNSPNGIMDFLFTEVFLNLKAHGYAQFSLGMVPLAQLGESEGLTTV
                     ERLLRWLLKRAPWLFRTGTLQRFKAKYASRWEARYSVYRNPLDLPKLALALRRVSELE
                     HIKEPA"
     gene            2568..3302
                     /locus_tag="VFQ79_01315"
     CDS             2568..3302
                     /locus_tag="VFQ79_01315"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0138313.1"
                     /translation="MKSAVLVLFAVVATQAAWGQHTPGIELYHLASSRSRQPVSVLFI
                     PGDGGWRGMAVKMAQNIAGWGYDVYGLDTKRYLEAFNRRPEKLTADQMSADLAEFARR
                     PGGGRSSVIIVGWSQGACMAVLAAARKETKPYIKGIVTLGLPEYGVLGWNWKDTLSMA
                     AGRPPEEPRFYVKDYLPHINPIPLFAIYGTNDDYTPPANERQMLSMVGRPMQLRLIEG
                     ANHRFDGHLDALFETLAEALRWVQQD"
     gene            3284..4276
                     /locus_tag="VFQ79_01320"
     CDS             3284..4276
                     /locus_tag="VFQ79_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU0138314.1"
                     /translation="MGTTGLTVDRLKPRAAPWKLGMRVIWFAVAAVCLWWVVRGIDFN
                     RLRSQILHVHSGWLALAVTCDVLGYVVQGIRWRLLLVSVGDLPVLTATKFIYAGLFAN
                     EVLPLRPGEILRGWLAAREMSVSPLAIAPSILAERLIDGVWLTAGLLLLAHFVRLPAG
                     LFHAVDLFSIAMMCLSAVAAVSRPAFAYLGVARRWHIFGNLRFVPGSLPVAGAWLGSA
                     VMLILQAFSFWFMMSACRIGLSWNEALGVLLVVRIGTLVPGAPANLGTYQLAVVLGLM
                     LFGVSKDVAAPFSVILFAALTAPLWILGAGAIAQTGVSLSKVRRGAAEWSHIAV"
     gene            4369..5685
                     /locus_tag="VFQ79_01325"
     CDS             4369..5685
                     /locus_tag="VFQ79_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU0138315.1"
                     /translation="MPRRISERTVLQGLALGFSIVILLLAASAFMAIRRSHVIRAAAA
                     ELLREQSTVVGIIDELQLTQQRMSTLLLDLFDDESGSPHLRKRSDDLAADILRVSTEG
                     ERVEPHPAWSKLKLTGEQFASTARQLLETPSPERGPVEYRLEELRTRFLALCNDVVRS
                     QAETAAGLEGQIEQQSMGLTRESALLLGGCLLLSLIGAAVTIRITQRSFRTMEWQAEE
                     LNRVSWHMLQTQEAAARRFSHEMHDELGQALAGLKANLMAVTPEEYAQRRQDSMHLLD
                     EAISKVRELSQLLHPVILDDFGIQAALRWLIDRFSERTRIEVDYESDVRGRLSEEIET
                     HLFRITQEALTNIARHSGATGAWIRFTTPGDSIRLTIEDNGCGLKPEFAREPGVGMVG
                     MRARARQIGGELTVGASVAGGVRLEVRAPRKEAETDDSQEDARVAG"
     gene            5651..6292
                     /locus_tag="VFQ79_01330"
     CDS             5651..6292
                     /locus_tag="VFQ79_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0138316.1"
                     /translation="MTPKKTRVLLADDHSIVRQGFRMILAGQPDFDVVGEAANGRHAV
                     ELAAELHPDVVVMDVSMPELNGIEATRRIGEISPRTRVIALSMHKDAVYVREILRAGA
                     RGYLLKDSMDTDLIGAVRAVARGEGYISPAVSDAVLNDYRKHVKDPLDLLSSREREVL
                     QLIAEGKTNKEIANGLKLSVYTVEAHRGRIMEKLNLHSAGEMVRFAIRNGLID"
     gene            complement(6308..7729)
                     /locus_tag="VFQ79_01335"
     CDS             complement(6308..7729)
                     /locus_tag="VFQ79_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEU0138317.1"
                     /translation="MTASRLLLSIILITWPVCVFGAANVVIVSGDAPGEGFSDPTPAV
                     PMGGNTGTTVGQQRLNVFRSVANIWGSTLTSSQTIRVLAFFDPLPCDVNSAVLGAAAP
                     YFTVANFGAGMPNTWYPISLAEKLANLDFGPALPPEDRFEVIALFNSDLGRTGCFDGS
                     GWYYGLDASSPGGLVNLATTVLHEFAHGLGFTVGPTNASTGARASGFPSIWEVYLRDL
                     TTRKLWLGMTDAERRASAVNTDNLVWSGGTSLSAAAAVLSLRPEVEILPPGPPVGAFE
                     AQPATFGPPVTPTGISGYLMPAIDAGGPSTLDACEPLTPQSAFSVNGRIALVDRGTCT
                     FTVKVLNAQNAGAIGALIANNVPTGLPAMGGSDPTITIPSLGITQALGQTLREQLRFR
                     GRAVSPVRVSLQRNPSIRSGTTAGYPRMFAPNPFQPGSSVSHWDISLDPNQLMEPFAT
                     PDITLSLTPPVDLTFPLLRDIGW"
     gene            complement(7719..8111)
                     /locus_tag="VFQ79_01340"
     CDS             complement(7719..8111)
                     /locus_tag="VFQ79_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138318.1"
                     /translation="MRITGQIILFLGLCGSMVLPAGPQNDSPEKAATGERSRETSGRR
                     VFRDPVTGLMRPPEASELAPSFGAAQRRVPSVVMRQLPNGGLARRTPLDEMDFLMVTR
                     GADGSLSYSCARKPEKGKTAGKEQALDR"
     gene            complement(8108..8806)
                     /locus_tag="VFQ79_01345"
     CDS             complement(8108..8806)
                     /locus_tag="VFQ79_01345"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HEU0138319.1"
                     /translation="MNLFSKMALCFGLSVSLQAAPFLVEGFADLDNLPGWVIANNSTP
                     GGATPEGWFQGTGVFPAQAGSPDSYAQANFENAGDPGDISTWLLTPVLTLRPQSVLRF
                     WTIGEDVPGFPDRLEVRLSTSGASTNVGSTPFSAGDFTTLLLSINPALDDAGYPTVWT
                     EYSYAFSALSSPVEGRVGFRYFVPDNTVSGSTIGLDSVSIVPEPASGILLSAGLGFFF
                     VLRNRRKQKREESL"
     gene            complement(8944..10011)
                     /locus_tag="VFQ79_01350"
     CDS             complement(8944..10011)
                     /locus_tag="VFQ79_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138320.1"
                     /translation="MQGTAVNDAPQSGEGIADRSLVQLTMVRFREFWREPEAVFWVFV
                     FPVLLTAGLGIAFRNQPAEVLKVGAVESTVSAALSREKRLSVQTMDRSAGETGLRTGR
                     ITLLVLPGEEGVVYRYDDTNPEARTARLLVDRALQRAAGAADLVRTSDDIVREPGSRY
                     IDFVVPGLLGMNLMGSGIWSLGFAVVDARRKKLLKRLVASPMRRWEYLLSFLLSRLVL
                     LSFEVAALVGFAVWIFHVPLRGSIGELALLCLVASLMFSALGLLIASRAQTIEAASGL
                     MNFVMLPMWILSGVFFSAERFPESVLPFIRALPLTAVIDALRANMLQGTPLAGMFWQL
                     AVMTVWGLLSFFVALRIFRWR"
     gene            complement(10016..10960)
                     /locus_tag="VFQ79_01355"
     CDS             complement(10016..10960)
                     /locus_tag="VFQ79_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0138321.1"
                     /translation="MQVPVTPLSLSIRGLRKSFGNVVAVDGIDVAASNGECLGLLGPN
                     GAGKTTTIEICEGLTQPDAGEVELLGLSWRHHAHELRQRIGIQLQETQFSDKLTVEET
                     LRLFRSFYRNGPSVFDLIAAVRMEEKRSARVVSLSGGQKQRLALACALAGNPDLLFLD
                     EPTTGLDPQARRQVWELVAELKHAGRTIILTTHYMEEAERLCDRVAIIDHGRIMALGT
                     PRELIASIGIGHVVEFAFDGVTGPLDLSALAALEGVRGAHAENGLYRLSVSELHRSVP
                     ALFEHLAGQGIELSELRTHSPTLEDVFVALTGRRLRDE"
     gene            complement(11015..12343)
                     /locus_tag="VFQ79_01360"
     CDS             complement(11015..12343)
                     /locus_tag="VFQ79_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0138322.1"
                     /translation="MQEFDRGGKTRRSFVQAGAVAAAGLGAQSLKAQSRETLALSGGP
                     KAVTFPSEKTAAILKWPRYGAEEKKAVADLLDSNGWYPEIPALEKELQQHLGAPYVKA
                     HMNGTSALMSMFFALGLPAGSEILAPSYTAWATTAPMHFFDYVPVFVDINPRTMTFDL
                     AYARKRLNSRIKAVLPMHSFGNPCDMDEISDFAKQHGLIVVEDAAQAQGASLKGKPMG
                     TWGSIGIFSFQGSKILPSIEGGAGVYQTREHYERATMFGNYELPNSFPPDSAYRVYQG
                     TGMGPKLRIHPLAAALARRQLRKMDEHNALVDGQLQKLNRRLSELPGISYPHTRPEAK
                     RVYWASNMIFIDEKKAGAPKAELLKALRAEGVQASPGAYDEQHRYKLYSEAKWWHHPV
                     VIPQDLHGTTQVNAQAVRLPVFHDEASELIEQYSQAFEKVWAHRSKLAGS"
     gene            complement(12369..12500)
                     /locus_tag="VFQ79_01365"
     CDS             complement(12369..12500)
                     /locus_tag="VFQ79_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138323.1"
                     /translation="MSERLAGPQGTCVDREIAVKVGFTAPLPLRLSYKFALVTGRGY"
     gene            12704..12792
                     /locus_tag="VFQ79_01370"
     tRNA            12704..12792
                     /locus_tag="VFQ79_01370"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12738..12740,aa:Tyr,seq:gta)
     gene            12823..12898
                     /locus_tag="VFQ79_01375"
     tRNA            12823..12898
                     /locus_tag="VFQ79_01375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12856..12858,aa:Thr,seq:ggt)
     gene            13008..>13151
                     /locus_tag="VFQ79_01380"
     CDS             13008..>13151
                     /locus_tag="VFQ79_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0138324.1"
                     /translation="MAKEKFERSKPHINVGTIGHIDHGKTTLTAAITKCLAKHNPKVK
                     FRSF"
BASE COUNT         2636 a         3929 c         4015 g         2571 t
ORIGIN      
        1 tcaccgatat gcccgggcag gctgtgtatc gtatcgacgc gccgggtaaa gtggtccggc
       61 tgctggcggc tcccgagatc cagaggccaa acgggattca agtagcgccg gatgatcgca
      121 cgctctacct catcgaagca aatggggccg aaggcggcgc ccgaatgatt cgcgcttacg
      181 acttgctgcc cgatggaagg gtccgcaaca tgcgtgttca ttacaacttc tatccgggcc
      241 gcagcgcgga cgggatgagc atcgatgtgg acgggaacct gtatgcgtcg gcgggcctgc
      301 accggcgccg cggtaccagc gaaaccctcg acacgaagtg cggggtgcat gtcatttctc
      361 cgcggggaaa gcttctgaag ttcattccgg tcccggaaga cacgatcacc aacaacgcgt
      421 tcggcggccc ggacgggaag acgctctaca tcacagcagg taagaccttg ttcaaagtgc
      481 gagtggaccg ccccggtttg ccgcgttaag cgccatcccc aacaatcgga tttcgcattc
      541 ccctctccag gctatgccgc cacttcggcc tcctgccgca tgcttgtttc agcgggggac
      601 cgaacccgga cctgctcaaa aaggagacaa caaacatgac acgcaagatc tggatggcgg
      661 ctgccttggc actcgccatg gtgggcggaa cgttcgcgca gacgccaccc tcccagccgg
      721 tcgagaagac cgcaaagacc gagaaagcga agaagcacaa gaaggtgaaa aagtcggaca
      781 agaagccctc gacatcgtcc acacaataac ctgcaccggg tcacaaagcg gtacacctgg
      841 aggatagaag acatgaggaa atgccgggtt gccctcccgg agaccgattc gccgccgccg
      901 gccagaagtg cagcgccctc cccgattaaa cggttggcta cggcggtagc gattgcggtg
      961 gctggcggca gcggtatcct caatcttctg tctctcctcg gtggaccggc gccggcggaa
     1021 aacccgagat ggctctggaa ggttctcccg ttcgattttg ttgggctttc ccgttccctt
     1081 acgctactgc tcgggttcat cctcatcctg accgcactcc atctatcgag tggaaaacgg
     1141 cgagcgcttc aactcgccat cggcctggcc ggcacttccg ggattctcca cctgatcaga
     1201 atggagctgc ccgaactcct ctcgtcgtcg gcaatcatcg ctctgttgct ttgggctcgc
     1261 gatctgttca cggctggaag tgcccggccg gctctgttgc ccgctgtacg ccgcgctctt
     1321 attgcgcttg cggttccctt tctttacggt gtaggcgggt tctggctgct cgagccgcac
     1381 gagtttggca aaaatttcca ctggtgggac gccatcgagc gaactgcccg gctgctggca
     1441 tttctcgacg ataagacact gctgccaaaa accgcctacg cagcctggtt tctcgactcg
     1501 atcacgctga ttctgcttgc atcgttcctc tatgcatgtt tgattctgtt ccggcccgcg
     1561 gcctatcact tccgcctgaa tcccgtcgaa caggaccgtg ccgccgccat cgccgaggat
     1621 tatgggcgtt ccgcgcagga ttacttcaag cactacccgg ataagagcta ctttttctcc
     1681 aaatcgggaa gggcttttat tgcctaccgg gtggcgggga acttcgcggt cgcactcggc
     1741 gatccggtcg gcccggaaca cgaattaccg gagatcgtcg aagacttcgc aggctactgc
     1801 cgggagcgcg gttggggcgt cgcattccac caggtttttc ccgaccgtct ttccatgtat
     1861 cgggcgcttg gattccggcg gctgaagatt ggtgacgacg cgctggtgga tcttcattcc
     1921 ttctcgctct ccggcagtgc ggcgaaagaa ttccgcaaca ccgtgaaccg gttgaagcgg
     1981 tacggctatc gcgtcgagcg gttcgatgcc ccaatcgaca gggagcgaat ggaggaactg
     2041 aagaacgttt ccgaagcctg gttaaagttg cccggccatc gggagcgccg gttcacactt
     2101 ggtaagttcg atcgggatta cgtccgctcg acgacggtat acgccgcact ggacgggcac
     2161 gggcatgtcg cggcattcct caatctggtg ccgtcctatt gccgcggcct cgccacggtc
     2221 gacctgatgc ggcgccatcc caactctccc aacgggatca tggactttct gttcactgaa
     2281 gtattcctga acctgaaagc gcatgggtat gcgcagttca gcctggggat ggtgccgctc
     2341 gcacagctcg gggagagtga gggtctgacg acggtggaac ggctgctccg ctggctcctg
     2401 aaaagagcgc cctggctttt ccgcaccggc acgctccagc ggttcaaggc aaagtacgcg
     2461 agccggtggg aagctcgcta cagtgtgtac cgcaatccgc tcgatctgcc aaagcttgcg
     2521 cttgcgttga gacgcgtgtc tgaactcgaa cacatcaagg aacccgcatg aagtccgccg
     2581 tcctcgtttt attcgcggtt gtggctaccc aggcggcatg ggggcagcac acgcccggga
     2641 tcgaattgta tcatctcgct tcctcccgga gccgccagcc agtcagtgta ctgttcattc
     2701 cgggtgatgg agggtggcgt ggtatggccg ttaaaatggc gcagaacatc gccggctggg
     2761 gctatgatgt gtacgggctc gacacgaaac gatacctgga ggctttcaac cggcggccgg
     2821 agaagttgac cgccgatcaa atgtcggccg atctggcgga atttgcgagg aggcccggag
     2881 ggggacgttc cagtgtcatc atcgtcggtt ggtcgcaggg ggcgtgcatg gccgttctgg
     2941 ccgcggcgcg aaaggaaaca aagccttaca tcaaagggat cgtaacgctg ggactccccg
     3001 aatacggcgt gctgggctgg aattggaaag acacactctc catggcggcc gggcgtccgc
     3061 cggaagagcc ccggttctac gtgaaggact accttcctca tatcaacccc attcccctct
     3121 tcgcgatcta tgggacgaac gacgattaca ctccgccggc gaacgagcgg cagatgctct
     3181 cgatggtagg ccggcctatg caactccggc tgatcgaagg cgccaatcat aggttcgacg
     3241 ggcatctcga tgccttattt gagacattag cggaggcgtt gcgttgggta caacaggact
     3301 gacggtagac cggctgaagc cgcgggcggc gccgtggaag ctaggcatgc gagtcatatg
     3361 gttcgcggtt gccgccgtct gtctctggtg ggtcgtccgc ggcatcgatt tcaaccggct
     3421 ccggagccag atcctgcatg tccattccgg ctggcttgcg ctcgcggtga cctgcgatgt
     3481 acttggttat gtggtacagg gcatacgatg gcggttgctg ctggtgtccg tgggagacct
     3541 gccggtcctc accgcgacga aattcatcta cgctggactc ttcgccaatg aggtgctccc
     3601 gttgcggccg ggagagatcc tgcgcggatg gctggcggca cgcgaaatgt cggtcagccc
     3661 gctcgctatc gccccatcga tcctcgccga gcggctgatc gatggtgtct ggctgacggc
     3721 cggactgctt ctgctggctc actttgtccg gcttcccgcc ggactcttcc acgcagtcga
     3781 cctcttctcg atcgccatga tgtgcctgtc cgcggtggcc gctgtgtcac gtccggcatt
     3841 cgcgtatttg ggagtggctc gacggtggca catcttcggg aatcttcgtt tcgttcccgg
     3901 cagtcttcct gtagccgggg cgtggctcgg ttccgcggtg atgcttattt tgcaggcttt
     3961 ctctttctgg ttcatgatga gcgcgtgccg gatcggcctt tcctggaacg aagcgctcgg
     4021 cgttctgctc gttgtgcgaa tcgggacgct ggttcccgga gcgccggcca atctcggaac
     4081 ctaccaattg gctgtcgtgc tgggcctcat gctattcggc gtatccaagg atgtagcggc
     4141 tccgttttct gtgatcctgt tcgcagcgct gacggctcct ctatggatac tcggcgccgg
     4201 cgcgattgca caaaccggtg tgagcctgag caaagtacgc cgcggggcgg ccgaatggag
     4261 ccatattgcc gtttgacgct cagtccccat gaagatccgg aggcgtcaac gtgtcctggc
     4321 aactacacta ggaagggaga cagtcttacg ggcacccttc gctcaccgat gccgcgccgg
     4381 atcagcgaac gaaccgtcct tcaaggtctc gcgctcggct tttccatcgt catcctgctc
     4441 ctggccgctt cggcctttat ggcgattcgc cgctcccacg tgatccgcgc cgccgccgcc
     4501 gaactgctgc gggaacagag cacggtggtg gggatcatag atgagctgca actgacgcag
     4561 cagcgtatgt cgacactgct gctcgatctg tttgacgacg agagcggctc accccatttg
     4621 cgcaaaagga gtgacgatct cgcggcggac atccttcggg tgagtacgga aggagagcgc
     4681 gtcgagcctc atccggcctg gtcaaagctg aagctgacag gagagcagtt cgcgtccaca
     4741 gcccgccagt tgctggagac gccttccccg gaacgtgggc ctgtagaata ccggctcgaa
     4801 gaactacgca cacggtttct agcgctgtgc aacgacgtgg tacggtcgca ggcggagacc
     4861 gccgcggggc tggaggggca gatcgagcag cagtcgatgg gtctgactcg cgaatcggcc
     4921 ttgctgcttg gaggatgcct gctactgtcg ctcatcggcg ccgccgtcac catccgcatt
     4981 acccaacgtt ctttccggac gatggagtgg caggcggaag agctcaaccg cgtgtcctgg
     5041 catatgctgc aaacccagga ggcggccgcg cgccggtttt cacacgaaat gcacgatgag
     5101 ctcggccagg cgctggcagg actgaaagcg aatctgatgg cggtcacgcc ggaagaatac
     5161 gcgcagcggc gacaggattc catgcacctg ctcgacgaag cgattagcaa ggtacgggag
     5221 ttgtcgcagt tgctgcatcc ggtcatcctc gacgatttcg gtattcaagc cgccctccgg
     5281 tggctgatcg accggttcag tgagcgaact cgcattgaag tcgattacga atcggatgtc
     5341 cgcggcaggc tctcagaaga gatcgagacg catctgttcc ggatcacaca ggaagcactg
     5401 accaacatcg cgcggcattc cggcgccacg ggggcatgga ttcgctttac cacgccgggc
     5461 gatagcatcc ggttgacgat tgaggataat ggctgcggcc tgaaaccgga gttcgcgcgg
     5521 gagcccggcg tggggatggt cggcatgcgg gcacgcgccc ggcagatcgg cggtgagctg
     5581 accgtcgggg cgagtgtcgc gggaggtgtg cggctcgagg tgagagcgcc gcgaaaggaa
     5641 gcggaaacgg atgactccca agaagacgcg cgtgttgctg gctgacgacc actccattgt
     5701 ccggcagggc tttcgcatga ttttagccgg ccaaccggac ttcgacgtcg ttggagaagc
     5761 tgccaatggc cgccacgccg ttgagctggc cgcggagctg caccccgatg tcgtcgtgat
     5821 ggacgtgtcc atgccggagc ttaacggcat tgaagcgacg cgccgcatcg gcgaaatctc
     5881 gccccgtacg cgtgtgatcg cgctgagcat gcacaaagac gccgtatacg ttcgtgaaat
     5941 tctccgggcc ggtgctcgcg gttatcttct caaggactcc atggacacgg acctgatcgg
     6001 cgcggtccgt gccgtcgccc ggggcgaggg ttatatcagc cccgcggtgt ccgacgcggt
     6061 tctaaacgat taccggaagc acgtcaaaga tccgctggat ctgctcagca gccgcgagcg
     6121 cgaagtactc caattgatcg ccgagggaaa gacgaacaag gaaattgcaa acggcctcaa
     6181 gctgagcgtc tacaccgtgg aagcgcaccg cgggcgcatc atggagaagc tgaaccttca
     6241 cagcgccggg gaaatggtcc ggtttgcgat tcgaaatgga ttgatcgatt gactgctacc
     6301 gccgcctcta ccagccgatg tccctcaaca gcggaaaagt taaatcgacc ggtggtgtga
     6361 gactgagtgt gatgtccggt gtggcgaatg gctccatgag ctgattcggg tccagagaaa
     6421 tatcccaatg agaaacggac gacccgggct ggaacggatt cggcgcaaac atgcgcggat
     6481 agcccgctgt ggttccggac cggatcgacg ggtttcgctg tagcgagact cgcacgggac
     6541 tcaccgcacg cccgcggaaa cgtagctgtt cccgaagagt ctgtcccaaa gcctgcgtaa
     6601 tgccgagaga cggaatcgtt atggtggggt cgctgccgcc catggcaggc aatcccgtgg
     6661 gaacgttgtt ggcgatgaga gcaccaatgg ctccggcgtt ctgagcattc aggaccttca
     6721 ctgtaaaagt gcaggttcca cgatcgacca gcgcgatgcg cccattgaca ctaaacgcgc
     6781 tttgcggcgt cagcggttca caggcgtcga gtgtggaagg tccacccgcg tcgatggcgg
     6841 gcatcagata tccggagatg cctgttgggg tgacgggcgg tccaaatgtt gccggctgcg
     6901 cttcgaatgc ccccaccggc gggccaggcg gcaggatttc cacctccggc ctcaggctca
     6961 ggaccgccgc ggcagcggac agagatgtgc cgccggacca cacgaggtta tcggtgttca
     7021 cggcggaagc ccggcgttcg gcgtccgtca tgccgagcca cagcttgcgc gtcgtcagat
     7081 cgcgcagata gacctcccag atggaaggga aaccggaggc gcgggcaccg gtcgaggcat
     7141 tggtcggtcc cacggtgaaa cccagaccat gcgcaaattc gtgaagaacc gtcgtcgcca
     7201 gattgaccag tccgccggga ctggatgcat ccagtccgta ataccagcca ctcccgtcga
     7261 aacacccggt tctacctaag tcgctattga aaagagcgat cacttcgaag cgatcctccg
     7321 gcggcaaagc gggaccaaag tcgaggttgg cgagtttttc cgcgagcgag atcgggtacc
     7381 aggtgttggg cattcccgcg ccgaagttgg ccacggtgaa gtaaggcgcc gccgccccca
     7441 gaacagccga gtttacatcg cagggaagcg gatcgaagaa agccagcacc cgaatggtct
     7501 ggctgctggt gagagtgctg ccccagatat tcgccacgga ccggaagacg ttcagccttt
     7561 gctgcccgac cgtggtgccg gtgttgccgc ccatcggaac agcgggcgtc ggatcgctaa
     7621 aaccttcacc cggtgcatcg cccgaaacaa tcaccacgtt ggccgcaccg aaaacgcata
     7681 ccggccaggt gatcaggatg atcgataaca acagacggct agcggtcaag tgcttgctcc
     7741 tttccggccg tttttccttt ctccggcttg cgtgcgcagg aataggacag gcttccatcg
     7801 gcgccccgcg tgaccatgag gaaatccatc tcgtcgagcg gagtccgtct tgccaggccg
     7861 ccattgggca actggcgcat caccacggag ggcactcgcc gctgcgccgc accgaacgaa
     7921 ggcgcgagtt ccgacgcttc cggaggacgc atcagcccgg tgacgggatc gcgaaacact
     7981 cttcgtccgg atgtctcgcg gcttcgctca cccgttgcgg ccttctccgg cgaatcattt
     8041 tgcggaccgg cgggcaacac catgctcccg cataaaccga gaaatagaat aatctgtccg
     8101 gtaattctca caacgattcc tctctttttt gtttgcgcct gttcctcaag acgaagaaga
     8161 agcccaaccc cgcgctgagt agaattcccg aagccggttc gggaacgatc gacaccgaat
     8221 cgagaccaat tgtcgagccg ctcacggtgt tgtccggaac aaaatagcgg aatccaacgc
     8281 ggccctcgac tggtgagctc aatgcggaaa atgcgtagct atattcggtc cacacggtgg
     8341 gatatccggc atcgtccaga gcggggttaa tggagagcag caacgtggtg aagtctccgg
     8401 ctgaaaaagg cgtcgagccg acgttggtgc tggcgccgct ggtactgagg cgtacttcca
     8461 ggcggtccgg gaacccggga acatcctcgc cgatagtcca gaaccgtaga acgctttggg
     8521 gcctcagcgt caatacggga gtcaacagcc aggtactgat atctcccgga tcaccggcgt
     8581 tttcgaaatt cgcctgggca taggaatcgg gggatccggc ctgcgccggg aatactcctg
     8641 tgccctggaa ccagccttcc ggtgtcgcgc caccaggggt gctgttgttt gcgatgaccc
     8701 accccggtaa gttgtcgagg tccgcaaatc cttccaccag aaatggcgct gcttgtaatg
     8761 aaacactcag gccgaaacag agtgccattt ttgagaaaag gttcaatgta cacctccgag
     8821 attttgtact ccagcattcc gcgttggtcc attgtggagt gagattccat atctgtcaac
     8881 gggtaaatgg cacaagtttc ccgagaatgt gcagccaatt tcgagagcgg atcggcaggc
     8941 ggcttaacgc cagcggaaga ttcggagggc gacgaaaaaa ctgagcaggc cccagacggt
     9001 catgactgcg agttgccaga acataccggc gagcggcgtt ccctgaagca tattcgcccg
     9061 cagcgcatcg atcaccgcgg tgagcggcag cgcgcggatg aacggcagca ccgattcggg
     9121 aaaacgctcc gccgaaaaga acacgcccga gagaatccac atcggcagca tgacgaagtt
     9181 catcaggccg gaggcggctt cgatggtttg cgcccgggaa gcgatcaaca aacccaacgc
     9241 gctaaacatg agcgaggcaa ccagacacag cagggcgagt tctcctatcg atccgcgcag
     9301 cgggacatgg aagatccaga cagcgaagcc caccagcgcc gccacctcga atgagagcag
     9361 aaccaggcgt gaaagcagaa acgacagcag atattcccaa cgccgcatgg gtgaagccac
     9421 caggcgcttc aacaacttct tccgccttgc atctacaacg gcgaacccca ggctccaaat
     9481 accactgccc atcaggttca ttcccagaag tcccggcaca acgaaatcga tgtagcggga
     9541 accgggctcc cgcacgatgt cgtcggacgt ccgcaccagg tcggcggcgc ccgcggcgcg
     9601 ttgcaatgcg cggtccacca aaagacgcgc ggtccgcgct tcgggattcg tatcgtcgta
     9661 gcggtagacg acgccctcct cgccgggcag gaccagaagc gtaatacgtc cggtgcgcag
     9721 ccccgtttcg ccggcggagc ggtccatggt ttgaaccgac aggcgtttct cgcggctgag
     9781 cgcggcggat accgtcgatt ccactgcacc tactttcagg acttccgccg gttgattgcg
     9841 gaacgctatc ccaagcccgg ctgttagcag aaccgggaaa acgaaaaccc agaaaaccgc
     9901 ctccggctcc cgccagaatt cgcggaaccg caccatcgtg agctggacca gagaccggtc
     9961 ggcgatacct tcgccggact ggggcgcatc gttcaccgca gttccttgca tccgttcatt
    10021 catcccgaag gcgccttccc gtgagcgcga cgaagacatc ttccagcgtg ggagaatgcg
    10081 tgcgcaattc actgagctcg atgccctgcc ccgccagatg ctcgaacaac gccggaacgg
    10141 agcgatgcag ctcggatacg gataatcgat agagcccgtt ctccgcatgc gcgccgcgaa
    10201 cgccttcgag cgctgccagc gcggaaagat ccaaaggacc ggtaacaccg tcgaacgcaa
    10261 attccacgac atgaccaatg ccgatggaag caataagctc gcgcggtgtt cccagggcca
    10321 tgatgcggcc gtgatcgata atcgcgacgc gatcgcaaag gcgttcggct tcctccatgt
    10381 agtgcgtcgt cagaatgatg gtccggccgg cgtgcttcaa ctccgccacc agttcccaga
    10441 cctgccgccg cgcctgcgga tcgagaccgg tcgttggttc atcgagaaac agcagatccg
    10501 gatttccggc taaggcgcag gccagcgcga ggcgctgttt ctgcccgccg gaaaggctta
    10561 ccactctcgc cgaacgtttt tcctccattc ggacggcggc gatcagatcg aagaccgaag
    10621 gtccgttgcg gtaaaagctt cggaacaggc gcaacgtctc ctccacggtc agtttgtccg
    10681 aaaactgcgt ctcctgaagc tggatgccga tgcgctggcg caattcgtgt gcatgatggc
    10741 gccaggagag gcccagcagt tccacttcgc cggcatccgg ctgggtaaga ccttcgcaga
    10801 tttctatcgt cgtggtcttg ccggcgccgt tcgggcccag caatcccagg cactccccgt
    10861 tggatgcggc cacgtcgatg ccgtcgacgg ctacgacgtt tccgaacgat ttccggagcc
    10921 cgcggatgga cagtgataac ggtgtgaccg gcacctgcat gaaccagtat ttttctaagt
    10981 ggggcctgcg ttcaactgag acgtgagaaa aggttcacga cccggccagc ttcgaccggt
    11041 gcgcccagac cttctcaaag gcctgagagt attgttcgat caactcggag gcttcatcat
    11101 gaaaaaccgg aaggcgtacc gcctgtgcgt tcacttgcgt ggtgccatgc aaatcttgcg
    11161 gaatcacgac gggatggtgc caccacttcg cttcggaata gagcttataa cgatgctgtt
    11221 cgtcgtaggc gccggggctg gcctgaacgc cttcggcacg cagcgctttg agcagctccg
    11281 cttttggtgc accggccttc ttttcgtcga tgaaaatcat gttgcttgcc cagtagacgc
    11341 gtttggcctc ggggcgcgta tgcggatagg aaatgcccgg cagctccgac aggcggcggt
    11401 tcagcttctg aagctgccca tccaccaggg cattatgctc gtccatcttc cgtaactgcc
    11461 tgcgcgcgag agccgcggcc agcggatgga tgcgcagttt cgggcccatg ccggtacctt
    11521 gatagacgcg gtaggcgctg tccggtggaa agctgttcgg gagttcgtaa tttccgaaca
    11581 tggtggcgcg ttcgtagtgc tcgcgcgttt gatacacccc ggctccgcct tcaatggagg
    11641 gtaggatttt gctgccctgg aagctgaaaa ttcctatcga gccccacgtc cccatgggct
    11701 tgcctttcag cgaagcgccc tgcgcctgcg cggcatcttc cacaacgatc agcccatgct
    11761 gtttggcgaa atcggagatc tcgtccatgt cgcagggatt gccgaacgag tgcattggta
    11821 gaacggcctt tatccggctg ttcaggcgct tcctggcgta agcaagatcg aaggtcatcg
    11881 tgcgcgggtt gatatcgacg aaaaccggaa cgtagtcgaa aaagtgcatc ggagccgttg
    11941 tagcccaggc cgtgtaggag ggcgcgagaa tctcgctgcc ggcgggcagg ccgagcgcga
    12001 agaacatcga catcagcgcg ctggtaccgt tcatgtgcgc tttaacgtac ggcgcaccga
    12061 gatgctgttg cagctctttt tcgagcgccg gaatttcggg gtaccagccg ttactgtcga
    12121 gcagatctgc caccgctttt ttctcttccg cgccataacg gggccatttg aggatggcgg
    12181 ccgtcttttc cgacggaaac gtcacagcct tcggaccgcc cgagagcgcg agagtttcac
    12241 gcgactgcgc tttgagcgat tgcgctccca gccccgctgc cgcgacggcc cccgcctgaa
    12301 caaaactccg ccgggttttg ccgccccgat cgaattcctg catgccgttt gcctccgaac
    12361 taaccctatc aatagccgcg cccggtgaca agcgcgaact tgtagctgag ccgcaacggc
    12421 aacggagcgg taaacccaac cttcacggcg atttcacggt ctacgcaagt gccttgagga
    12481 cccgccaacc gctcgctcac gctcacggct cagctacaag ccgtggccgc acgcccagcc
    12541 gttggaggtg ttaagctgaa ggaaacttta cccggcaccg cttgctaacg taggctcggg
    12601 aatcgtgttc cgctggacac tgtgaggtgg actgcgataa cctgagagct gaccctgcga
    12661 acctgaggat ctgtagtcct ccgcctacaa agcgcgttat tccggacggg tggccgagtg
    12721 gttaatggca gcagactgta aatctgccgc tccttgcgag ctacggaggt tcgaatcctc
    12781 ccccgtccac caggtccgtt tcagtggggg tcgagttttt gggccgttgt agctcagttg
    12841 gtagagcgcg tccttggtaa ggacgaggtc accggttcaa tcccggtcaa cggctccata
    12901 atttcggatg ccgccggagc acctgaaggg gaggcggccg gttgaatttc ccgcctgaag
    12961 ggttcgaact cataacaggc aacgataaat ttccgcgagg ataagcgatg gcgaaggaga
    13021 aattcgagcg cagcaagccg cacataaacg tggggacgat cgggcatatc gatcacggca
    13081 agacgacgct gacggcggcg atcaccaagt gcctggcgaa gcataatccg aaggtgaagt
    13141 tccggagttt c
//
LOCUS       DASTMK010000036         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_162219, whole genome shotgun sequence.
ACCESSION   DASTMK010000036 DASTMK010000000
VERSION     DASTMK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162219"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFQ79_01385"
     CDS             <1..1243
                     /locus_tag="VFQ79_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138325.1"
                     /translation="VQPLIGRTFTDDEDRHSTQVALLSYRLWQRSFGSDLSVIGRTVY
                     LDRNPYTVLGVMPSGFEFPLYGLSSRGPVDLWVPVSFTPAELKDIGNNYSYGLLARLK
                     TEATLEQAKADSEAIARRIWQDDLSGLPGAELHAVLTPVPEAVTGKVATLLWLLLGAV
                     GLVLLIACANVSSLLLARATTRQKEIAIRVALGAGCVRLLRQFLTESVLLAATGASLG
                     LLLAAWVLRLFVRFSPGYLPRLHEIAIDWRILLFTIAITSVTAVIFGLLPALAMSGRQ
                     LNDPLKESARGATTGRGRLRLRNTLVAAELALAFVLLAGAGLLIRSFAVLRATDPGFQ
                     AEHVLGLSLRLPEAVYSAAPQVRSFYQELLDRIAVLPGVRIAAAGTSLPMASDQHDMG
                     NGKLFPGPWHSVAARPHFFAG"
     gene            1179..2021
                     /locus_tag="VFQ79_01390"
     CDS             1179..2021
                     /locus_tag="VFQ79_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0138326.1"
                     /translation="MGNYFQALGIPLLRGRTFSPADTKSSTPVVMIDQDIAARFWPGQ
                     DPVGKRLKWGAGGNSGEWITVAGVVGNVRNAPFDEESLLSTYLPYSQAPDSRVLGNGR
                     AMRIIVRTNVNPESVAASLRAAVRSFDSQIAVADLRTMKEVIGRSMAVRRFNMILLGI
                     FAGLALVLAIVGVYGVIAYSVAQRTHEIGVRIALGARRADVLRTILKQGGWLAAIGIG
                     AGLAGASIVTRLMSSLLFGVEPGDSVTLMAASLILFAVAMLATLLPACRAAQVNPVEA
                     LRSE"
     gene            complement(2163..>3864)
                     /locus_tag="VFQ79_01395"
     CDS             complement(2163..>3864)
                     /locus_tag="VFQ79_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138327.1"
                     /translation="YGLDRAAANVPFGSLGCCQGGYNLAGWLMGYPSTSSTAEGLNWT
                     GARQNRWGAYFQDEWKVSRRLTANLGLRWDYFETPHDPRRKWRALRLDILNTASDGRM
                     LPTVVPEPLADFKFYEADKRYFMPRVGLAFRATEKWVIRTGGGWYVNVQQLNNMTILA
                     LLPPFSGTYSYNAVTDAASVIPIQYAGQTYNIQTRKFRPGSQVLTLDDPFPGQGTAAA
                     RTNVSVFPPDNRPSSVWQWSFDIQRELPAKTFLTVAYVGSKTSHIDNTIPNFNSPDPS
                     PDTDINSRRPWQAYVSQGEGDQPLLLGNIRYLDSYANGNYHALQVSANKRYSHGLTVG
                     LAYVYGKARGEGYGRNDPSGDVNSTYQDPRNRRNDRMRYGFDVTHNAVINYVYDLPAM
                     RGSHSLVRAILGGWQTSGIVTLRTGTPFTVNGGTLNTGGPSWPDRIADGRLGGKATRE
                     LWYDPAAFRRTECNIPGRLDLCHYGNAAPDALVSPGMRSFDLSVGKNWAISPLGDNAR
                     LQFRAEAFNAFNTPQFGRPNNLAWASLDSVVPDAPRVGEIRSLRTPMRIFQMGLKLYF
                     "
BASE COUNT          700 a         1151 c         1208 g          805 t
ORIGIN      
        1 cgtacagcct ctcatcggcc gtacgttcac cgatgacgaa gaccgccata gtacacaggt
       61 cgcgctgttg agctacaggc tgtggcagcg aagcttcggg tcggatctct ccgtcatcgg
      121 cagaacggtc tatctcgacc gtaatcctta tacggtgctc ggcgtaatgc caagcggatt
      181 cgaatttcca ctttatggcc tctccagccg gggaccggtg gatttgtggg tgccggtatc
      241 gttcacgcct gccgaactga aggacatcgg gaacaactac tcgtacgggc tgctggcgcg
      301 cttgaaaaca gaagcaaccc tggaacaggc caaggcggac agcgaagcga tcgcgcgccg
      361 gatctggcag gacgacctat ccggcttgcc gggagctgaa ctccatgccg tgctcactcc
      421 cgtgccggaa gcggttacgg gcaaggtggc cacgctgctg tggctgctgc ttggagccgt
      481 ggggttggtg cttctgatcg cctgtgccaa cgtctccagt cttctgctcg cacgggccac
      541 aacgcgccaa aaggagatcg cgattcgagt cgctttgggc gccgggtgcg tgcgcctgct
      601 gcgtcagttc ctgaccgaga gcgttttgct cgcagccacc ggcgcttcct tgggactgct
      661 cctggccgca tgggtactcc ggctgttcgt tcgtttctct ccaggctatc ttccacgcct
      721 gcacgaaatc gctatcgatt ggcgcattct tctattcacg atcgcgatca cctccgtcac
      781 cgcggtgata ttcgggttgc tgcccgcctt ggcaatgtcc ggccggcaac taaacgatcc
      841 gctgaaagaa agcgcccgtg gagcgacgac aggccgcggc aggctgcggt tacggaacac
      901 cctggtggcg gccgagttgg ctctggcttt cgttctgctg gctggagcgg gactgcttat
      961 ccggagtttc gctgtgctgc gtgcgacaga ccctggattc caggcggagc atgtcctcgg
     1021 tttatcgctg cgtctgcccg aagcggtcta tagcgccgct ccgcaggttc ggtcattcta
     1081 tcaggagctg ctggatcgaa tcgcggtgtt gcccggcgtg cgcattgccg ccgccggaac
     1141 gtccctcccg atggcctcgg accaacacga catgggtaat gggaaactat ttccaggccc
     1201 ttggcattcc gttgctgcgc ggccgcactt tttcgccggc tgacacgaaa tcctctacgc
     1261 cggtggtaat gatcgaccag gatatcgctg cgcgattctg gccggggcag gatcccgttg
     1321 gcaagcggct caaatggggc gcggggggca actccggtga gtggatcacg gtggccggcg
     1381 tggttggcaa cgtcaggaac gcaccgtttg acgaagagtc gctgctgtcc acctatctgc
     1441 cctattcaca ggcaccagac tcccgcgtgc tcggcaatgg ccgggccatg cgcatcatcg
     1501 tgcggaccaa tgtgaatccc gaaagcgtgg cggcgtcgtt gcgggcagcc gtccggtcct
     1561 tcgacagcca gatcgccgtg gcagacctgc gcacgatgaa ggaagtgatc ggccggtcga
     1621 tggcggtgcg gcgcttcaat atgatcctgc tcggcatatt cgccggtttg gcgctggtgc
     1681 tggcgattgt gggtgtttat ggcgtgatcg cctattcggt ggcgcagcgc acgcacgaga
     1741 ttggcgtccg aatcgccctc ggcgcgcgca gagccgatgt gttgcgaacc attctgaagc
     1801 agggcggatg gcttgccgcc atcggaatcg gcgcggggct agccggagct tcgattgtga
     1861 cgcggttgat gtcgagcttg ttgttcggcg tcgagcccgg cgattccgtc accctgatgg
     1921 ccgcgtcgct gatcctgttt gccgtggcca tgctggccac tcttctgccg gcctgccgtg
     1981 ccgcgcaggt gaaccccgtg gaagctttac gctcggagtg aaaatgtttg cacgacgatt
     2041 tgattggcca cggatggaca cagatgaaca cagataagaa aaatccgtgt gaatccgtgt
     2101 ttatccgtgg ccgatttgct tttctagatt gctgggatgc gcaatcattt ctgacgccac
     2161 gtttaaaaat acagtttcag acccatctga aaaatgcgca tcggcgtgcg caaggaacgg
     2221 atttcaccca cgcgcggcgc gtcgggaacc accgaatcca gggaggccca cgccaggttg
     2281 ttgggccgcc cgaactgcgg tgtattaaaa gcattgaacg cttcggcgcg gaactgtagc
     2341 cgggcgttgt cgccgagcgg cgagatggcc cagttcttgc cgaccgacag atcaaaactg
     2401 cgcattcccg gagagactag ggcatccggc gccgcattgc cgtagtggca aagatccagg
     2461 cggccgggaa tgttgcattc ggtgcggcga aacgcggctg gatcgtacca caactcccgc
     2521 gtcgccttgc cgcccagacg gccatccgca atccggtccg gccatgaagg ccctcccgtg
     2581 ttcagtgtgc cgccatttac cgtgaaagga gttccggtgc gcagcgtaac gatgccggag
     2641 gtctgccacc cgcccagaat cgcccgcacc aggctatgcg agccgcgcat cgccggcaga
     2701 tcgtacacgt agttgatcac cgcgttgtgc gttacatcga agccataacg catgcggtcg
     2761 ttgcggcggt tccgggggtc ctgataagta gagttcacat cgcctgaagg gtcgttgcgg
     2821 ccataacctt cgccgcgcgc tttgccgtat acgtaggcga gaccgacggt aagcccgtgg
     2881 ctgtagcgct tgttcgcgct cacctgcagc gcatggtagt ttccgttggc gtagctatcc
     2941 agataccgta tgtttccgag caggagcggt tggtctcctt cgccctggct gacataggcc
     3001 tgccatggcc ggcggctgtt gatgtcggta tccggtgatg gatccgggct gttgaagttc
     3061 ggaatggtgt tatcgatgtg gctggtcttg ctgcctacgt aagctacggt caggaacgtc
     3121 ttagcgggca gttcgcgctg aatatcgaag ctccactgcc agacactgct cgggcgatta
     3181 tcgggcggga acaccgacac gttcgtacgg gcggccgcag tcccctgccc ggggaatggg
     3241 tcgtccaacg tcagaacctg cgagcccggg cgaaacttgc gtgtctgaat gttataggtc
     3301 tgaccggcat attgaatcgg gatgacgcta gccgcatccg tcaccgcgtt gtagctatac
     3361 gtccccgaga agggcggcag caacgccaat atcgtcatgt tgttcaattg ctgcacattc
     3421 acataccacc ctccgccggt gcggatgacc cacttctcgg tggcgcggaa ggccaaaccg
     3481 acgcgcggca tgaaatagcg cttatccgct tcgtaaaact tgaagtccgc gagcggctct
     3541 ggaacaaccg taggcagcat ccggccgtcg gaagcggtgt tgaggatatc cagacgaaga
     3601 gcccgccact tgcggcgcgg gtcgtgcgga gtctcgaagt aatcccaacg caggccgaga
     3661 ttcgcagtca gccggcggct cactttccat tcgtcctgga agtacgcgcc ccagcggttt
     3721 tggcgagcgc ccgtccagtt caggccttcc gccgtgctcg aggtcgatgg atagcccatc
     3781 agccagccgg ccagattata gccaccctgg cagcagccca aactgccaaa gggaacattg
     3841 gccgcggcac gatccaaacc gtat
//
LOCUS       DASTMK010000037         5501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_66391, whole genome shotgun sequence.
ACCESSION   DASTMK010000037 DASTMK010000000
VERSION     DASTMK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66391"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFQ79_01400"
     CDS             <1..351
                     /locus_tag="VFQ79_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0138328.1"
                     /translation="LSGDDAVTLPVIALGGRGIISVVGNEIPGEMTRLAQLALQNDFP
                     AAREIQRRYLPLMEVNFVESNPIPVKAAMALMGLLEPVWRLPLVAPQPASQAKIEKVL
                     QAVGLLADRGVHAG"
     gene            341..1177
                     /locus_tag="VFQ79_01405"
     CDS             341..1177
                     /locus_tag="VFQ79_01405"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEU0138329.1"
                     /translation="MRADLELLFDQPPSAYTEEHFRLFQRFKDALNQGEIRAAEPDAG
                     AASGWRVNGWVKKGILLGFRMGALVDMSVDAARQPFFDKATYPVKRLSPSSGVRIVPG
                     GSSIRDGCYVGKGVTCMPPMYINAGSYVSEGTMIDSHALVGSCAQIGKSCHISAASQI
                     GGVLEPVGALPVIIEDEVLVGGNCGVYEGTVVKRRAVLGTGAILNRSTPVYDLVHEQV
                     YTASDEEPLVIPEGAVVVAGSRAIPKGKGKEWGLSLYTPVIVKYRDARTDAKIQLEDI
                     LR"
     gene            complement(1157..2269)
                     /locus_tag="VFQ79_01410"
     CDS             complement(1157..2269)
                     /locus_tag="VFQ79_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0138330.1"
                     /translation="MAALRIGVNALYLIPGGVGGTEIYLRSILAAMAGIDSENEYFLF
                     TNRETGADLVPAAPNFHSCPQAVSARSRPARILWEQTALPLALVKTRIDVLFNPGFTA
                     PLLCPCPMVTVFHDMQHKRHPEYFRWFDLPFWRMLLLQAAVESHTLIAVSEATRNDIL
                     KYYRLPEKKIRVIPHGVDPLLFHIGERTGNFILCISTLHPHKNLERLIRAFARFHHAY
                     PEFRLVLAGLRGFHAEEIERLIKSLDLGAAVEITGWIPREQLRDLFRRAWAFVYPSTF
                     EGFGMPVLEALAAGVPSACSDIEPLTSISGEAALHFDPGDENSIANALAILAGDAEVR
                     ERLIAAGPLRARQFSWETTARETLNALRGALTQDVL"
     gene            complement(2270..4018)
                     /locus_tag="VFQ79_01415"
     CDS             complement(2270..4018)
                     /locus_tag="VFQ79_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0138331.1"
                     /translation="MKKPARRQQSRPAPPAVSVPPRRGWKWWQWALCAAALLFLVFEV
                     YAPALGGPFLFDDVYLPFAMPGVEKLSFTEWLQGVRPMLMLTFWMNYNLSGTQPLSYH
                     VFNLLFHLVNAILLFFLVRRLLSRAGESGFTRDFLAAFAGAVFLLHPVQTESVAYVAS
                     RSESLSLLFFFAAFAAFLYRRSEVIGFGSSAIVVLLFGCALLVKEHAAVLPVLLLLTD
                     YYWNQPPFSLQGIRRNWRLYVPIMVLTVGGGVFVWGVLQTADTAGFRVEGLTWYEYFF
                     TQWRAIWVYIRLFVLPFGQSLDYAYPISRTPLDHGAILGLVGLLLLAGAAIYYRRRFP
                     LASYGFFAFLILLAPTSSLVPIKDPLAERRLYLPMIGLLLILVDLLRRWRISRVQLAA
                     ACVAVLVVLSALTYQRNKVWSDPILLWQDTVAKSPHNSRAHFQLAYAYYQSGRCAESV
                     RAYEETAQLTEPDYRLLVDWALADDCNNQPAEALAHLEQAAALEKSAHVYSLIGMVHA
                     KQKRYPEAMTALDTAIHLDPDYSMTYVYRGNIHALQGDYRKAVEEYRRALAADPSNQP
                     ARDALAVAESNLKQGF"
     gene            complement(4015..4404)
                     /locus_tag="VFQ79_01420"
     CDS             complement(4015..4404)
                     /locus_tag="VFQ79_01420"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEU0138332.1"
                     /translation="MDRLLDFVAVFGGVLMLLVLLSLRRAHIRVEYSVSWLVAAFVLI
                     VISRFEGTIRAASGFLGIDYPPLALLLIVLSVFLVVFFRFSMIISKLKDSNIALAQRV
                     AILEFELRSGAQPTVASGQQADPGAGL"
     gene            complement(4407..5138)
                     /locus_tag="VFQ79_01425"
     CDS             complement(4407..5138)
                     /locus_tag="VFQ79_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0138333.1"
                     /translation="MRHRPEPGALLVVVPAYNEEAAIGNVVREIREVMPRVPVLVIDD
                     CSVDSTRAAAADAGAYILSLPHHLGLGGCVQAGYKLAFELGYDYVIRVDGDGQHDPRY
                     IPKLYEALRTSGCEMVIGSRFAAGNGQPTSFARGVGIRFFRTVLRPILGKSVYDPTSG
                     FVGVNRQALAVFSKSFPLEYPEIEALVVLQRRAFNFVEVPCEMRPRRGGQSTITAWKS
                     LYYIIHVLLGVFVNILKFEHWRWRR"
     gene            complement(5135..>5501)
                     /locus_tag="VFQ79_01430"
     CDS             complement(5135..>5501)
                     /locus_tag="VFQ79_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138334.1"
                     /translation="HSAGRASPLKAYYVERNRLFVAVKNFPAGMLWKAPFVSALRYLW
                     HLVYMFRGRGAGAEFRGGHSALLLPWYVLRAHMALAGSLPGLWRERRRIRTRARISPS
                     EFGRLAAIHRISARQVAQL"
BASE COUNT         1215 a         1653 c         1708 g          925 t
ORIGIN      
        1 ctttcgggcg acgatgcggt gacgctgccc gtgatcgcac ttggagggcg cggcatcatt
       61 tcagttgtgg gcaacgagat tccaggtgaa atgactcgcc tcgctcagct cgcgcttcaa
      121 aacgattttc cggcggcgcg ggaaatccag cggcgttacc tgccgctgat ggaagtgaac
      181 ttcgtggagt ccaatcctat tccggtgaag gcggccatgg ctctgatggg tttgctcgaa
      241 cccgtgtggc gcctgccgct ggtggcgccg cagccggcca gccaggcgaa aatcgaaaag
      301 gtgctgcaag ctgtgggact gcttgccgac cggggtgtgc atgcgggctg atctcgaatt
      361 gctgttcgac cagcctccat cggcttacac tgaagaacat ttccggctgt tccagcgttt
      421 caaagacgct ttgaaccagg gcgaaatccg tgcggccgaa ccggacgccg gtgcggccag
      481 cggatggcgc gtgaacggct gggtcaagaa gggcatcctc ctcggtttcc gcatgggtgc
      541 gctggtcgat atgtccgtgg atgcggcacg tcaaccgttc ttcgacaaag caacgtaccc
      601 ggtgaagcgg ctgagtccct cgagtggcgt gaggatcgtg cccggcggct ccagcattcg
      661 cgacggctgt tacgtcggca aaggcgtcac ctgcatgccg ccgatgtaca tcaacgcggg
      721 gtcctatgtc agcgaaggca caatgatcga ctcgcacgcg ctggtgggaa gctgcgcaca
      781 gatcggaaag agttgccata tctcggccgc ctcacaaatc ggaggcgttc tcgaacctgt
      841 aggcgcgctg ccggtcatca ttgaagacga agtgctggta ggcggcaatt gcggcgtgta
      901 cgaaggaacc gtggtaaaac ggcgcgcggt gttggggacg ggcgccattc tgaatcgatc
      961 cactcccgtc tacgatctcg tgcacgagca ggtctacacg gcgagcgatg aggagccgct
     1021 tgtgattccg gaaggcgccg tggtcgtggc cggttcgcga gccatcccaa aaggcaaagg
     1081 gaaggagtgg ggcctctcgc tttacactcc cgtcatcgtc aagtaccgcg atgcacggac
     1141 cgacgccaaa atccagctag aggacatcct gcgttaaggc gccgcggaga gcgttcagcg
     1201 tctcgcgcgc ggtggtctcc caggagaatt gccgggcgcg aagcggaccc gcagcgatga
     1261 ggcgctcgcg gacttctgca tcgccggcca gtatcgcgag cgcgttggcg atgctgttct
     1321 cgtcgccggg atcgaagtgc agcgccgctt cgccgctgat actcgtcagg ggctcgatgt
     1381 ccgagcaggc ggacggaacg cccgcggcga gtgcttcgag aaccggcatt ccaaagcctt
     1441 cgaacgtgga agggtagacg aaagcccatg cgcgccgaaa caggtcgcgc aactgctcgc
     1501 gcggaatcca accggtaatt tcaaccgctg ctcccagatc gagcgatttt atcagccgtt
     1561 cgatctcttc ggcatgaaat ccgcgcaggc ccgccagcac cagccggaac tccgggtacg
     1621 catggtggaa gcgggcgaag gcgcggatga gccgctccag gtttttgtgc gggtgcagcg
     1681 tggaaatgca aaggatgaaa ttgccggtac gctcgccgat gtggaacagc agcgggtcca
     1741 ctccgtgggg gatgacgcgg atcttctttt cgggcagccg gtaatatttc agaatgtcgt
     1801 tccgtgtggc ctcggatacg gcgatgagcg tgtgcgattc gaccgccgcc tgcagcagca
     1861 gcattctcca gaacggcaga tcgaaccagc gaaagtactc cggatgccgt ttgtgctgca
     1921 tgtcgtggaa gacggtcacc atcggacacg gacacagcag cggcgccgta aagccggggt
     1981 tgaagagcac atcgattcgt gttttgacca aagcgagcgg cagcgctgtc tgctcccaca
     2041 ggattcgcgc ggggcgggac cgggcgctga cggcctgcgg gcaagagtga aagttcggcg
     2101 cggcgggaac caggtccgct cccgtctcgc ggttcgtgaa gaggaaatat tcgttctcgc
     2161 tgtcgatgcc cgccatcgcc gccaaaatgc tgcgaagata gatctccgtg ccgcccaccc
     2221 cgcccggaat cagatacagg gcgttcacac cgatgcggag cgccgccatt caaaagccct
     2281 gcttcaggtt gctttccgcc acggcgagcg catcgcgcgc aggctggttg gaggggtcgg
     2341 cggcaagggc ccggcgatac tcctccacgg ccttccggta gtcgccctgc aatgcatgaa
     2401 tgttgccgcg gtagacgtag gtcatcgagt agtcaggatc caggtgaatg gctgtatcga
     2461 gcgctgtcat ggcttcggga taccgcttct gtttcgcatg caccataccg atgagcgagt
     2521 acacgtgagc gctcttttcg agtgcggctg cctgttccag gtgcgccagc gcctccgcgg
     2581 gctgattgtt gcagtcatcg gcgagcgccc aatccaccag caggcggtag tcgggttcgg
     2641 tgagttgtgc ggtctcttcg taggcacgaa ccgattccgc acagcgtccc gattgatagt
     2701 acgcatacgc cagttggaaa tgcgcgcgcg agttatgcgg agatttggcg accgtgtcct
     2761 gccacaacag gatcggatcg ctccaaacct tgttgcgctg atacgtcaga gcggataaga
     2821 ccacgagaac ggccacgcac gcggcagcca gttgcacgcg cgaaatgcgc cagcgccgca
     2881 gcaggtccac caggatcagc agcagtccga tcatcggcag atacaggcgg cgctcggcca
     2941 gcgggtcttt gatgggcacc agcgaggacg taggggccag caggatcaga aacgcaaaaa
     3001 agccgtacga agccagcgga aagcggcggc ggtaatagat ggccgctccg gcgagcaaca
     3061 gcaaaccaac gagccccaga atcgcgccat gatcgagcgg cgtgcgcgag atgggatacg
     3121 cataatccag gctttgtccg aagggcagca caaagagccg gatgtaaacc cagatggcgc
     3181 gccactgcgt gaagaaatac tcgtaccagg tgagcccttc tacgcgaaat ccggcggtgt
     3241 ccgcagtttg caggactccc catacaaaga cgcccccgcc cactgtaagc accattattg
     3301 gaacatacag acgccagttc cggcggattc cctgaagcga gaacgggggc tgattccagt
     3361 agtaatccgt cagaaggagc agaacgggca acaccgccgc gtgttctttc accaggagag
     3421 cgcagccgaa aagcagcaca acgatggcgg acgaaccgaa tccgatcact tccgagcggc
     3481 ggtagaggaa cgcggcgaaa gcggcgaaaa agaaaagaag gctcagggat tccgagcggc
     3541 tggccacgta cgcgacagac tcggtttgaa cggggtgcag cagaaacacc gcaccggcaa
     3601 aagcagcgag gaagtcgcgt gtaaagccgc tttcacccgc ccggctcagc aatcgtctca
     3661 caaggaagaa aagaagaatg gcgttgacca gatggaacag gagattgaaa acgtgatacg
     3721 aaagcggctg ggtgccggag agattgtagt tcatccaaaa cgtcagcatc agcatcggcc
     3781 ggacgccttg cagccactcc gtgaacgaaa gcttctcgac gcccggcatc gcaaacggaa
     3841 gatacacgtc atcgaacagg aaggggccgc cgagagcggg cgcgtacacc tcgaacacaa
     3901 ggaacagcag agcagccgcg cacagtgccc actgccacca tttccagccg cgcctgggag
     3961 gcacactcac cgcggggggc gccggcctcg attgctgccg gcgcgccggt ttcttcacaa
     4021 tcccgctccc ggatccgctt gctgcccgga ggccaccgtt ggctgcgcgc ccgatcgcaa
     4081 ctcaaactcc aggatggcaa cccgctgcgc cagcgcaatg ttggaatcct tcagcttcga
     4141 gatgatcatg gaaaagcgga aaaagacgac caggaagacc gacaaaacga tcagtagcag
     4201 cgccagcggc ggatagtcga ttccgagaaa ccccgatgcc gctcggattg taccttcaaa
     4261 ccgcgagatg acgatgagta caaatgcggc gaccagccag ctcaccgaat actccacgcg
     4321 aatgtgcgcg cgccgcagcg acagcagcac cagcagcatt aggacaccac cgaaaaccgc
     4381 cacgaaatcc agcaggcggt ccatagctat ctcctccatc gccaatgctc aaatttgagg
     4441 atattcacga agactcccaa aaggacatga ataatgtagt acagcgattt ccatgcggtg
     4501 atggtgctct ggcccccgcg ccttggccgc atctcgcaag ggacctccac gaagttgaaa
     4561 gcgcggcgct gtaacaccac aagcgcttcg atctccggat attcgagtgg aaagcttttg
     4621 ctgaaaaccg ccagcgcctg gcggtttaca ccgacgaagc ccgatgtcgg atcgtacacg
     4681 gacttaccca ggataggccg cagcacggtg cggaagaacc ggataccaac accgcgagca
     4741 aacgacgtcg gctgcccgtt gccggcggca aaccgggatc cgatgaccat ctcgcacccg
     4801 gacgttcgca acgcttcgta caacttcggg atatagcgcg ggtcatgctg cccgtcgcca
     4861 tcgacgcgga tcacgtaatc gtagcctagc tcaaatgcga gcttgtagcc cgcttgcaca
     4921 cacccgccga gtcccaggtg atgcggcaag gacaggatgt aagcaccggc gtccgccgcg
     4981 gcagcccgcg tggaatccac ggaacaatcg tcgatgacca gcaccggcac gcgcggcatc
     5041 acttcgcgga tttcacggac cacattccca atggcagcct cttcgttata agcgggcaca
     5101 accaccagca aggcgccggg ctccggcctg tgcctcacag ttgtgccacc tgtctggcgg
     5161 aaatgcggtg tatagctgcc agccggccga attcggaggg agaaattcga gcccgggttc
     5221 gaatacgcct ccgctcccgc cagagaccag gcaacgatcc ggcgagcgcc atgtgggcac
     5281 gcaacacgta ccatggcagc agcaaggcgc tatggccgcc gcgaaactcc gcgcccgctc
     5341 ccctgcctcg aaacatgtac accaggtgcc aaaggtaacg gagtgcggag acgaatggcg
     5401 ctttccacag catgcccgcc gggaagttct tcaccgccac aaaaagacgg ttacgctcca
     5461 cgtaatacgc tttcagcggc gacgcgcggc cggcggagtg t
//
LOCUS       DASTMK010000038         7047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_130242, whole genome shotgun sequence.
ACCESSION   DASTMK010000038 DASTMK010000000
VERSION     DASTMK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130242"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VFQ79_01435"
     CDS             complement(<1..1226)
                     /locus_tag="VFQ79_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138335.1"
                     /translation="MFALAILASLEIIQVGPETALWATPAILGAAMLIAWAAESAQFF
                     VAQGFALAILAWLQTLPEFAVEAILAWKQQVPLLLANLTGALRLLTGLAWPMIYFTAA
                     MFYRKRTRRALGHIQLEDEHSIEVCALLVPLAYFAFIWWKASLGLIDALVLILIYAAY
                     LLLLSKLPPQREESIEELEMIPRKIVLSPKNTRRFLILGLFTIGGLLIYFTAEPFLGS
                     LLALSAMLGIPSFVFVQWIAPFVSEFPEKVSAFYWARTVERASMALMNMVSSNINQWT
                     LLAAMLPIVFSISRGEPSSIPFDGRQELEIAMTLGQQLLGMVFLLNMQLLWWEAAALF
                     SLWGIQFAFSPVETGPGLWGYIAGHIHAWVTYVYFLWVAVEVVRMAIRREMPLAFRLF
                     AMMWKRHVRGGKAAIE"
     gene            complement(1273..2841)
                     /locus_tag="VFQ79_01440"
     CDS             complement(1273..2841)
                     /locus_tag="VFQ79_01440"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEU0138336.1"
                     /translation="MYHGNIGPTVEHITYDFLVVGAGVAGLMAAIELAPVGKVLVVAK
                     DSLRESSSEYAQGGIAAALSDDDEVELHEQDTIAAGDGLCDRSAVRTLVEEGPASIQR
                     LIEWGAEFDREGSRLAFAREGAHSRSRVLHAHGDSTGREISRTLYQKAASLENVTFRS
                     FSAITDLLVRDGEVVGAVACDQAAKQSVAIAARAVLLATGGLGRLYRDTTNPDVATGD
                     GVATAYRAGAEISDIEFVQFHPTALHVEGAPRFLLSEALRGEGAYLRNAAGERFMEKY
                     HPMAELAPRDVVSRSIISEIGKTGAPHVFLDLTHLDAAFVRKRFPRIYETCLHHGVDI
                     SKEPAPVHPAAHYAMGGVRTDLNGQTSLRRLFAAGEVACTGVHGANRLASNSLLEGLV
                     FGARAGKTMRALADEEIQRGEIPGECRFPALDEDKLRGIASERCGIIRCRAGLEQACA
                     ELLAAGSVPRKDAVRAHYETRNMQTVTLLIARCALARQESRGAHFRGDFPAKDTAFQR
                     HSLITNNKNEVEFV"
     gene            2849..3922
                     /gene="aroB"
                     /locus_tag="VFQ79_01445"
     CDS             2849..3922
                     /gene="aroB"
                     /locus_tag="VFQ79_01445"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU0138337.1"
                     /translation="MPSYVVETPQQRYFAKVERGILSRVPYYVPDSAGKIFIITTRDV
                     WDLYGGRLRRGLTDRAHEVLFFPGGEQNKRLAAVETLAEEMIAAGGDRSSIVVAFGGG
                     IVNDVGGFVASIFMRGIPVIQVPTTLLAQVDAAVGGKTGVNLASGKNLVGRFHQPHCV
                     LIDPDVLASLPEREYRAGLYEVIKCGIIRSSWLFNLMSKEAKSILAHEPRVVESMIAE
                     CVRIKAEVVSADEKEGDLRRILNFGHTVGHALEAETGYTRFLHGEAVAFGMNAAVHLA
                     NLLDMLSPDECLSVLETVRLYGPIPSLRGISADALVARLQQDKKTVKGRVHFVLPEKI
                     GRVRVVSDVDEELVLSAVRSALK"
     gene            3919..4680
                     /gene="ubiE"
                     /locus_tag="VFQ79_01450"
     CDS             3919..4680
                     /gene="ubiE"
                     /locus_tag="VFQ79_01450"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEU0138338.1"
                     /translation="MSRGTTPPGVSSEEQAASWVRGMFNRVAPRYDLLNHLLSFQMDR
                     WWRAQTVSRLHPVLRRPNVRVLDICCGTGDLTIALANKQAGEVFGSDFSFPMLLEAAQ
                     KVKRTGKQAALFEADALSLPLRDASLDVIAVAFGFRNLANYQRGLAEMRRVLKPGGVA
                     AILEFSTPPNAVFRAFYNLYSRKILPVVGGLISGSRDAYRYLPESVRKFPGAETLAQQ
                     MAEAGFDSVQFLRMTGGIVALHTGVRSAAPDYSGG"
     gene            complement(4651..6063)
                     /locus_tag="VFQ79_01455"
     CDS             complement(4651..6063)
                     /locus_tag="VFQ79_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0138339.1"
                     /translation="MTANYQLQLNAEEMRAIGYRIIDLIVERHRTLPDQPATRKESPQ
                     ELSRRLHEPLPEHGCSMDTILDQVLDDVLSNCSRLDHPRFFGFVPSPSNFIGAMADAL
                     ASGFNIFAGSWISGSGAAALELVAVDWMRQLCGMPESAGGLFVSGGSMANLTGLALAR
                     HTMLDDRTGGAVAYLSDQTHASVERALRVLGFLPCQISELATDDRFRLRVEDLAAAVA
                     RDRSAGKRPFCVIANAGTTNTGAVDPLAELSRFCREENLWLHVDGAYGAAAVLCERGR
                     TLLEGLESVDSLSLDPHKWLFQPFESGCILVRRGSLLASTFRILPEYLADIHRGGEDV
                     NFGDRGIQLTRGFRALKLWMTIKYFGLEAMRKAVGRGFELAEAAEAEIRKTRDFEIVT
                     PAQMGVVTFQHSGARDPEFYRRLVDGMAAGGLAFLTSTVLRGETVLRLCTINPRTSEA
                     DLVQTLDRLRELAAGIIGSG"
     gene            complement(6060..6356)
                     /locus_tag="VFQ79_01460"
     CDS             complement(6060..6356)
                     /locus_tag="VFQ79_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HEU0138340.1"
                     /translation="MRLRKAQGKLKVEHSIIDGVRAMLERLLGENPGVRAVIPGVIRK
                     VRDAKGEVQVRVTVPVQNGWKAIAFSAGARQELFISTTLDKSELEAALERARRP"
     gene            6386..>7047
                     /locus_tag="VFQ79_01465"
     CDS             6386..>7047
                     /locus_tag="VFQ79_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0138341.1"
                     /translation="MRQSLIIDADDTLWENNIYFERAFEAFVAYLDHSKLSAEEIRAI
                     FDEMEAVNNKTRGYGSLNFGRNLQQCYEHLCEREFSDVNLDKVMDFAHAIIDQPVELI
                     DGVKDTLEYLVTRHDLTLFTKGHPEEQKLKIDRSGMGVYFGHTAIVKEKDSASYRQLV
                     AERRLDTGITWMIGNSPRSDINAALEAGLNAVFVPHARTWILEKQALLEGPGKLLKLE
                     SFR"
BASE COUNT         1411 a         2153 c         2113 g         1370 t
ORIGIN      
        1 tgttcgatgg cggccttgcc gccccggacg tgtctcttcc acatcatcgc aaacagccgg
       61 aatgcaagcg gcatctctct gcgaatcgcc atccgtacga cctcgaccgc cacccatagg
      121 aagtagacat aggtaaccca cgcgtgaata tgtccggcga tgtaacccca cagtcccggc
      181 ccggtctcga cgggggagaa agcaaactgg attccccaca aggaaaacaa tgctgcggcc
      241 tcccaccaga gcaattgcat gtttagaaga aacaccatgc ccaatagctg ttgtcccagg
      301 gtcatagcaa tctccagctc ctgccggccg tcgaagggaa tgctggaagg ctcaccgcgg
      361 ctgatggaga agacaatcgg cagcatcgcc gccagaagag tccactggtt gatgttgctc
      421 gacaccatat tcatcagtgc catcgaggcg cgttcgaccg tgcgggccca gtaaaacgcc
      481 gaaaccttct ccggaaactc ggacacgaaa ggcgcgatcc actgaacaaa tacgaaactg
      541 ggaattccca gcatggccga gagcgcgagc aggctgccaa ggaacggttc cgctgtgaaa
      601 tagatcagta atccgccgat ggtgaagagt ccgaggatga gaaagcggcg ggtatttttc
      661 ggcgaaagca cgatctttcg cggaatcatc tccagttctt cgatgctttc ctcgcgctgc
      721 ggcggcagct tgctcaacaa cagcaggtag gcggcgtaaa tcagtatcag caccagcgcg
      781 tcgatcaacc cgagggacgc tttccaccag atgaaagcaa agtaggccag cggtacaaga
      841 agggcacaca cctcgatgga atgttcgtct tcgagttgga tatggcccag cgcacgcctc
      901 gtccgcttgc gataaaacat tgcggcggta aagtagatca tcggccatgc gagaccggtt
      961 agcagccgca aggctcctgt aagattcgcg agcagcagcg ggacctgctg tttccaggcg
     1021 aggatggctt ccaccgcgaa ctcgggcagc gtttgcagcc aggccagtat ggcgagcgcg
     1081 aaaccttgcg ccacgaagaa ctgggccgat tccgccgccc aggcaatcag catggccgcg
     1141 cctaaaatgg cgggagtggc ccacagggcg gtttccggcc ccacctgtat aatttcaagc
     1201 gatgcaagga tcgccaaagc aaacaccgga ggtgccacct ggcgggacct tatcggtatc
     1261 cgcgatgtga attcagacga attcgacttc atttttgttg tttgttataa gagagtgccg
     1321 ctgaaacgcg gtatcttttg cggggaaatc gcccctgaag tgcgcgcccc ggctctcctg
     1381 ccgcgccagg gcgcagcggg cgatcagaag cgtcacggtc tgcatgtttc tcgtttcgta
     1441 atgggcacgc acggcatcct tccggggaac actaccggcg gccagaagct cagcgcaagc
     1501 ctgctccagt cctgcgcggc agcggataat gccgcagcgt tccgaagcaa tgccgcgcag
     1561 tttgtcttca tccaaagccg ggaaacggca ctccccgggg atttcgcccc tctgaatctc
     1621 ttcgtccgcc agagcgcgca ttgtcttgcc cgcgcgcgcg ccaaaaacca ggccttccag
     1681 aagagagttg ctggccagcc ggttggcgcc gtgaacgccc gtgcaggcga cttctcccgc
     1741 ggcgaagagt ctgcgcagac tggtctgtcc attcagatcc gtgcgtactc cgcccattgc
     1801 atagtgcgcc gcgggatgca ccggcgccgg ctctttcgag atgtcgacgc cgtggtgcag
     1861 gcacgtttcg tagatgcgtg gaaagcgctt gcgcacaaac gcggcatcca gatgagtcaa
     1921 atccaaaaag acgtgcggtg cgcccgtttt tccgatttcg gagatgatgg agcgcgatac
     1981 aacatcgcgc ggcgcgagct cggccatcgg atgatacttt tccatgaacc gttcacccgc
     2041 cgcgttccgc agatatgcgc cctcgcctcg cagcgcttcg gacagaagaa atctcggtgc
     2101 gccttcaacg tgcagagccg tggggtggaa ctgaacgaat tcgatgtcgc tgatctcggc
     2161 gcccgcgcgg tacgcggttg ccacgccgtc tcccgtagct acgtcgggat tcgttgtgtc
     2221 gcggtagagc cgccccaacc cgccggtggc cagcagaacc gcccgcgccg cgatcgccac
     2281 ggactgtttc gcggcttgat cgcacgccac ggcacccacc acctctccgt cccgaaccag
     2341 cagatccgta atggcggaga agctgcggaa cgtgacgttc tcgagagagg cggccttctg
     2401 gtataacgtc cgggatatct cgcgccccgt ggagtctccg tgggcgtgca gtacccggct
     2461 gcggctgtgg gccccttccc gggcgaacgc cagccgcgag ccttcgcggt cgaactcggc
     2521 gccccattca atcaggcgtt ggatggaagc gggtccttcc tccaccaggg ttcgcaccgc
     2581 actgcggtcg cacaggccat ctccggcggc gatggtgtcc tgctcatgca gttccacctc
     2641 gtcgtcatcg cttaaggccg cggcgatacc accttgcgca tattccgatg aggattcccg
     2701 aaggctgtct ttggcgacga cgagcacctt gcccaccggc gccagttcaa tcgccgccat
     2761 gagtccggcg acgcccgctc caaccaccag gaaatcgtat gtaatgtgtt cgactgtcgg
     2821 tccgatgttc ccatggtaca actttcatat gccttcctat gttgttgaga cccctcagca
     2881 gcgatacttt gccaaggtcg agcgtggcat tctctcccgg gtgccgtatt acgtgccgga
     2941 ttccgcgggg aaaattttta tcatcactac gcgcgacgtg tgggacttat acggcggaag
     3001 gctgcgccgc ggattgacgg accgggcgca tgaggtgctg ttctttccgg gtggagaaca
     3061 gaataagcgc ctcgctgccg tcgagacgct cgccgaagag atgatcgccg ccggcggcga
     3121 tcgttccagc atcgtggtgg catttggcgg cggcatcgtg aatgacgttg gcggtttcgt
     3181 cgcgtcgatc ttcatgcgcg gcataccggt gattcaggtg ccgacaacgc tcctggccca
     3241 ggtggatgcc gcggtcggtg gaaagaccgg tgtcaacctc gcctcgggca aaaacctggt
     3301 cggccggttc catcaaccgc actgtgttct gatcgatccc gacgtgctcg ccagccttcc
     3361 ggaacgcgag tatcgcgccg gcctttacga agtgatcaag tgcggcatca tacgcagctc
     3421 ctggctgttc aacctgatgt cgaaagaggc aaaatccatt ctggcgcatg agccgcgggt
     3481 tgtggagtcg atgattgcag agtgtgtccg catcaaagcc gaagtggtat cggccgacga
     3541 gaaagaaggc gacctgcgcc gcatcctgaa ctttggacac acggtcggcc atgcccttga
     3601 ggccgagaca ggctacacgc gattcctgca cggggaggcg gtggctttcg gcatgaacgc
     3661 ggccgttcat ctggccaacc tgttggacat gctttccccg gacgaatgtc tgtcggtatt
     3721 ggagacggtg cgcctctacg ggccgattcc gtccctccgt ggaatctccg ccgatgccct
     3781 ggttgcacgt ttgcagcagg acaagaagac ggtgaaaggg agagttcatt tcgtgctgcc
     3841 ggaaaaaatc ggcagagtgc gggtggttag cgacgtcgac gaggaactgg tgctttccgc
     3901 cgtgcggtcc gctctgaaat gagccgtggg accacgcctc ccggtgtctc cagcgaggaa
     3961 caggcggcga gctgggttcg cggtatgttc aaccgggtcg cgccccgtta cgatcttctg
     4021 aatcacctgc tctcgtttca gatggaccgt tggtggcgcg cgcaaacggt gagccgcttg
     4081 cacccggtgc tccgccgccc gaacgtgcgc gtgctggata tttgctgcgg caccggagat
     4141 ctcaccatcg ccctcgccaa caaacaggcc ggcgaggtgt tcggcagcga tttttccttc
     4201 ccgatgctgt tggaagccgc gcaaaaagta aagcggacag gaaaacaggc ggcgcttttc
     4261 gaagcggacg cgttgtcgct tccgctgcgc gatgcttcgc tcgacgtgat tgccgtggcc
     4321 ttcggtttcc gcaatctggc caattaccag cgcggccttg cggagatgcg gcgggtgctg
     4381 aagccggggg gagtagcggc gattctcgaa ttctcaactc cgccgaatgc cgtgttccgc
     4441 gcgttctaca acttgtattc gcgaaagatc ctgccggtcg tgggcggtct gatttccgga
     4501 tccagggacg cctaccgcta cctgccggag tctgtccgga agttcccggg cgcggaaaca
     4561 ctggcacagc agatggcgga agccggtttc gattccgttc agttcctgcg catgacggga
     4621 ggcattgtcg cgctgcacac cggagttcga tcagccgctc ccgattattc cggcggctag
     4681 ttcacgaagc cggtcgagtg tttggaccag gtccgcttcg gacgttctgg gattgatcgt
     4741 gcagagccgg agcacggttt cgccgcgcag cacggtagac gtcagaaacg ccagcccgcc
     4801 ggccgccatc ccatccacca gccggcggta gaactccgga tcccgcgcac cgctgtgctg
     4861 gaacgtgaca actcccattt gcgcgggagt gacaatttcg aaatcgcgcg tcttgcgtat
     4921 ttccgcttcg gctgcctcgg ccagttcgaa gcctctaccg acggctttgc gcatcgcctc
     4981 cagcccgaaa tacttgatcg tcatccacag cttcagcgcg cgaaaaccac gtgtaagctg
     5041 aatgccgcga tcgccgaaat tcacatcttc gccgccccgg tggatgtcgg cgagatattc
     5101 cggcagaatg cgaaacgtgc tggccagcag cgacccccgg cggacaagaa tacacccgct
     5161 ttcaaaaggc tgaaaaagcc atttgtgggg atcgagcgac agggaatcga cgctctccag
     5221 cccctcgagc agcgtgcgcc cccgttcgca aagaaccgcc gctgcgccgt atgcgccgtc
     5281 gacatgcagc cagagattct cctcccggca gaaacgggaa agctccgcca gcggatcgac
     5341 cgcgcccgta ttggtcgttc cggcgttcgc gatcacgcaa aacgggcgtt tgccggcgct
     5401 acggtcgcga gcgacagccg ccgcgagatc ctctacacgc aggcgaaatc tgtcgtccgt
     5461 tgccagctcg gagatctggc aaggaagaaa gccgagaacg cgaagtgccc tctccaccga
     5521 agcgtgtgtc tgatcgctca ggtacgcgac tgcgccgccg gtacgatcgt cgagcatggt
     5581 gtggcgggca agggcaagcc cggtcaggtt ggccatggaa ccaccgctta cgaaaagtcc
     5641 gccggccgac tcgggcatcc cgcagagctg ccgcatccaa tcgaccgcga ccagctccag
     5701 tgcggccgcg ccggacccgc tgatccacga tcccgcgaag atgttgaatc ccgatgccag
     5761 cgcatccgcc atggcaccga taaaattcga gggcgatggc acaaagccga agaagcgcgg
     5821 atggtccagc cgcgagcagt ttgagagaac atcgtcgagc acctgatcga gaatcgtatc
     5881 catcgaacag ccgtgctcgg gtagcggttc gtgaagccgg cgggacaatt cctgcggcga
     5941 ttctttgcgg gtagcaggct ggtccggcag cgtgcggtgc cgctcgacaa tcaggtcgat
     6001 gatgcggtag ccaatggcgc gcatctcttc cgcattaagc tggagctggt agttcgccgt
     6061 cacggccgcc tcgcacgttc cagcgccgct tcgagctcgg acttgtcgag agtcgtgctg
     6121 ataaacagtt cctgtcgcgc tcccgcactg aacgcgatgg ctttccatcc gttttgaacc
     6181 ggcacggtga cgcgcacctg tacttcgcct tttgcatccc ggactttccg gatcacgccc
     6241 ggaattaccg cacgcacgcc gggattttcc ccgagcagcc gttccagcat cgcgcgcacg
     6301 ccgtcgatga tactgtgctc taccttcaac tttccctgcg cttttcgaag tcgcacggct
     6361 ttagtgtact ttgggatttt gtaacatgcg acaatccctc atcatcgatg cggatgacac
     6421 gctctgggaa aacaacatct acttcgaacg ggcttttgaa gcgttcgtcg cttatttgga
     6481 tcactcgaag ctcagcgcgg aagagatccg cgccatcttt gacgagatgg aggcggtgaa
     6541 caacaagacg cgcggctacg ggtcgctgaa cttcggccgc aatctgcaac agtgttatga
     6601 gcacctctgc gagcgcgagt tctcggacgt gaatctggac aaggtaatgg atttcgcgca
     6661 tgcgattatc gaccagccgg tagagctgat cgacggtgtc aaggatacgc tcgaatacct
     6721 ggtgacgcgg cacgacctga ccctgttcac caaaggccat ccggaggaac agaagctcaa
     6781 aatcgaccgt tcggggatgg gggtttactt cggccacacg gccattgtga aggagaaaga
     6841 cagcgcgtct taccggcagc ttgtagcaga acggcggttg gatactggca ttacctggat
     6901 gatcgggaat tcgccgcgct ccgacatcaa cgccgcgctc gaagccggac tgaacgccgt
     6961 atttgtgcct catgcccgca cctggatcct ggagaaacag gcgctgctcg aaggccccgg
     7021 gaaactgcta aagctcgaaa gcttccg
//
LOCUS       DASTMK010000039         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_225989, whole genome shotgun sequence.
ACCESSION   DASTMK010000039 DASTMK010000000
VERSION     DASTMK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225989"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFQ79_01470"
     CDS             complement(<1..868)
                     /locus_tag="VFQ79_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0138342.1"
                     /translation="MSWRERLFAGAVIPAHPLALNAERKLDERRQRALARYYLAAGAG
                     GIAVGVHTTQFEIRDPKVGLFRPVLELAMEEMRGKDVVAVAGVCGQTRQAVAEAEFAA
                     SVGYNAGLLSVGAMRDATPAQLIEHARAVASVIPVFGFYLQPAVGGRVLPYEFWRGFA
                     EIENVIAIKIAPFNRYQTIDVIRAVADSGRADSIALYTGNDDTILADLLTPYQFNGKE
                     LRIKGGLLGHWAVWTRRAVEQIDRVHKAIEAGGRIGTDWLTLATQITDSNAAFFDPAH
                     NFHGCIAGLHEVL"
     gene            complement(868..1887)
                     /locus_tag="VFQ79_01475"
     CDS             complement(868..1887)
                     /locus_tag="VFQ79_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0138343.1"
                     /translation="MIASESQLEDILSRPSDADVEAMRSMEGDLLVLGVAGKMGPSLV
                     HRAKRAISQAGINKRVIGVSRFSSPAARDELKDMGVETIAADLLNEADLAALPDIPNV
                     IFMAARKFGTTGAEHLTWAMNTYLPGRVAERFRHSRIVAFSSGNIYPLVPIAEGGAKE
                     STPVQPVGEYATSVLGRERLFQHFSDKYGTPVVLLRLNYAVELRYGVLCDIGRKVFER
                     RPVDLNMGCANVIWQGDANSVCLRSFSLCSSPPAVLNLTGPETVSVRQVALQFGAHFG
                     IDPIFEGSEAPNALLNNAGKCHRLFGYPSVSVEQMIEWISQWIGMGGATLDKPTHFEA
                     RDGKF"
     gene            complement(1887..2369)
                     /locus_tag="VFQ79_01480"
     CDS             complement(1887..2369)
                     /locus_tag="VFQ79_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HEU0138344.1"
                     /translation="MEIKVIRLEIPDSTNLILGQTHFIKSVEDLYEAIVNTVPQAKFG
                     IAFNEASGPCLTRVVGNDAELRETASRNATAVAAGHIFVLILRDAFPINVLNRIKDVP
                     EVCSIYCATANPVEVIVAETSQGRGVLGVVDGFPPKGVEGEDDVKARHGFLRKIGYKL
                     "
     gene            2405..>3610
                     /locus_tag="VFQ79_01485"
     CDS             2405..>3610
                     /locus_tag="VFQ79_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEU0138345.1"
                     /translation="MPVKPAAFDDNELLRYIGQLPHGRATFKQLVREHSARGDNRKKL
                     EDSLDRLTASGALLELRSGHYVSVSSSREYAAGRLRMHRDGYGFLIADKPLDWIRGDV
                     FIPAESARKAMHGDQVVVRIGRVDQEGRAEGEIARILRRAHATVVGEFRVRRHGNIVV
                     PHDDRIQQWIEIPEELEMPAASISVDRIGVEPVTIKGPEDLDGMIVNVELLDYPDDGE
                     HAVGRVIEILGHPDDFGIDVEIVIRKHHIPHQFPPEVLHQAQQVPATIRQEEIASRRD
                     FRNLDIVTIDGETARDFDDAVWIDRLPKGNFVLQVHIADVSYYVRPGTPIDLEAALRG
                     TSVYFPDRAVPMLPLELSTEICSLKPQLDRLVLSVILEIDHRGDILRQEFHRGIIRSV
                     ERMTYTNVHL"
BASE COUNT          766 a         1147 c         1004 g          693 t
ORIGIN      
        1 gcagaacctc gtgcagaccg gcgatacatc cgtggaagtt gtgagccgga tcgaaaaatg
       61 ccgcgttgct gtcggtgatc tgggttgcca gcgtcaacca atcggtgccg atccgccctc
      121 cggcctcgat cgccttgtga actctgtcaa tctgctccac ggcgcggcgt gtccagacgg
      181 cccagtgccc taacagaccg ccttttatac gaagctcttt cccgttgaac tgatacggcg
      241 tcaacaaatc cgccaggatc gtgtcatcgt ttcccgtgta cagcgcgata gagtccgccc
      301 ggcccgaatc ggctacggcg cgaatgacat cgatggtctg ataccggttg aacggcgcta
      361 tcttgatcgc gattacgttc tcgatttccg cgaagccccg ccagaactcg tacggcagca
      421 cgcgcccgcc aaccgcaggc tgcagataga aaccgaaaac gggaatcacg cttgccaccg
      481 ctcgggcatg ctcgatcaac tgtgcgggag tcgcatcgcg catcgcgcct acgctcagca
      541 ggccggcgtt ataaccaacc gaagcggcaa actccgcctc cgctacggcc tggcgggtct
      601 ggccacagac gcccgcgacg gcgacaacat ctttgccgcg catctcctcc atggcaagtt
      661 ccagaaccgg ccggaacaat cccactttcg gatcgcgtat ctcaaactgc gtggtgtgca
      721 ccccgacagc gattccgccc gcgcctgccg caagataata acgcgctaaa gcgcgctgcc
      781 gccgctcatc caatttgcgt tcggcgttga gcgccagcgg gtgcgccgga atcaccgcgc
      841 ccgcaaagag cctctcccgc cagctcatca gaacttaccg tccctggctt caaaatgcgt
      901 gggcttgtcg agagtggctc cccccattcc gatccactgc gagatccatt cgatcatctg
      961 ctccacgctg acgcttgggt agccgaacag acggtgacat ttgccggcgt tgttcaacaa
     1021 ggcgttcggg gcctccgatc cttcaaagat cggatcgatg ccaaagtgcg cgccgaattg
     1081 aagcgccact tgacgcaccg aaaccgtctc cggcccggtc aggttcaaca ccgcgggcgg
     1141 cgaagaacac aagctgaaag accgcaggca aacggaattg gcatccccct gccagatcac
     1201 gttcgcgcat cccatgttga gatccaccgg acggcgctca aagactttgc ggccgatatc
     1261 gcaaaggacc ccatagcgaa gctccaccgc atagttcaga cggagcaaca ccactggagt
     1321 cccgtactta tcggagaagt gctggaacag ccgttcgcgc cccagcaccg acgtggcata
     1381 ttcccccacg ggctgcacgg gcgtcgactc ttttgcgccc ccctccgcga ttggtaccag
     1441 cggatagatg ttcccgctgg agaaggcaac gatacgggaa tgccggaacc gctcggccac
     1501 acgtcccggc aggtacgtgt tcatggccca cgtcaggtgc tcggcgccgg tcgttccaaa
     1561 tttcctggcc gccatgaaaa tgacattcgg gatgtcgggc agggccgcga gatccgcttc
     1621 attcagcaag tcggcggcta tcgtctcaac gcccatgtcc ttcaattcat cgcgggccgc
     1681 gggcgacgag aacctggaaa ctcctattac gcgcttgttg atgccagcct gcgagatggc
     1741 gcgcttggcc ctatggacca ggctggggcc catcttcccg gcaacgccca gcacaagcag
     1801 atctccctcc atgctccgca tggcttccac atcggcgtcc gaaggacgcg acagtatgtc
     1861 ttccagttgt gattcagatg caatcatcag agcttatatc caatctttct taaaaatccg
     1921 tgccgggcct tgacgtcgtc ttcaccctcc acccccttgg gggggaatcc gtcgaccacg
     1981 ccaagtactc ccctgccctg gctggtctcg gctacaatca cctcaacagg attggcagtc
     2041 gcgcagtaga tggagcagac ttccggtaca tctttgatcc ggttcagcac attaatggga
     2101 aaggcatcac gcaagatgag gacgaagatg tgcccggccg ccacagcggt cgcattccgg
     2161 cttgcagtct cacgcagttc cgcatcattg ccgaccacgc gtgtcaggca aggtcccgaa
     2221 gcttcgttga aagcaatgcc gaatttggct tgcggaaccg tattgacaat agcctcgtac
     2281 aggtcctcta cgctcttgat gaagtgcgtc tggccgagga tgaggttcgt cgagtccgga
     2341 atctccagac ggatgacctt tatctccatg gatttaccag gatactacgg ataatagaaa
     2401 taagatgccc gtcaaacccg ctgcgttcga cgacaacgaa ctgcttcgct atatcgggca
     2461 gttgccccat ggaagagcta ccttcaaaca attggtgcgg gaacattccg cccggggcga
     2521 caaccgcaaa aaattggaag actcactgga ccgcctcacg gcgagcggcg ctctgctcga
     2581 attgcgttca gggcactacg tgtcggtgag ttccagccgc gaatacgcag ccggccgcct
     2641 caggatgcat cgtgacggct atggctttct gatcgcagac aaaccgctcg attggatccg
     2701 gggcgatgtg tttattcccg ccgagtctgc ccggaaagcg atgcacggcg atcaggtcgt
     2761 cgtgcgcatc ggacgcgtgg atcaggaagg acgcgcggaa ggcgaaatcg cacgcattct
     2821 gcggcgagcg cacgcgaccg tcgtcggaga gttccgcgtc cggcgccacg gcaacattgt
     2881 cgtgccgcac gacgaccgca ttcagcaatg gatcgagatt ccggaagaac tggagatgcc
     2941 ggcagcgagc atcagcgtgg accgcatcgg cgtggaaccg gtcacgataa aaggcccgga
     3001 agacctggac ggcatgatcg tgaatgtcga actgctggac tatccggacg acggtgaaca
     3061 cgccgtgggc cgcgttatcg aaatcctcgg ccatccggat gacttcggca tcgatgtgga
     3121 aattgttatt cgcaagcacc acatcccgca ccagttcccg ccggaagtat tgcatcaggc
     3181 ccagcaggta cccgcgacaa ttcgccagga ggagatcgcc tcccgccgcg atttcagaaa
     3241 cctggatatc gtgaccatcg acggtgagac cgctcgcgat ttcgacgacg cggtatggat
     3301 agaccgcctg cctaagggca atttcgtcct tcaggtgcac atcgccgacg tgagctacta
     3361 tgtccgcccc ggtacgccca tcgacctcga agccgcgctt cgcggtacga gcgtctactt
     3421 ccccgaccgc gcggtcccca tgcttccgct ggagttatcc acggaaatct gctcgttgaa
     3481 accccagttg gaccggctgg tgctctcggt gattctcgag atcgaccacc gtggcgacat
     3541 cctccgccag gaattccatc gcggaatcat ccgaagcgtg gaaaggatga cgtacacaaa
     3601 cgttcatctc
//
LOCUS       DASTMK010000040        19039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_130328, whole genome shotgun sequence.
ACCESSION   DASTMK010000040 DASTMK010000000
VERSION     DASTMK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19039
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130328"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFQ79_01490"
     CDS             <1..540
                     /locus_tag="VFQ79_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138346.1"
                     /translation="NRYGSELATAVTEKISGDLRAISGPLRTAFYIEELDFATPPTRD
                     ELKKKLSHENVYERRHAEEMLKIMDRDGRLPAKYSYPLQVWQFGPNLTLIAMAGEVVV
                     DYNLRLRKELGEENTWVAGYSNDVSAYIPSLRILKEGGYEGGGAMIYYVQPGPFAPNV
                     EELIMERIHKLVRQVRPAK"
     gene            complement(547..3759)
                     /locus_tag="VFQ79_01495"
     CDS             complement(547..3759)
                     /locus_tag="VFQ79_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0138347.1"
                     /translation="MCSVSTVFRGATLIAAFLTIPLGAQEAGSIQGRVVDPQGATVPN
                     VKVTLEQEGTGNTRSAIAGSDGLYSFAIVPVGIYTITAEAPGFKRAIVPSVRVEVAQR
                     VQMDFALEIGAVTETVQVTSSAPQLQTADSQIGAVVETKAITDLPLNGRNFTQLMVLM
                     AGSTERSGGTVAGHYGERAAGTAFSVNGQRQTANQFLINGFMAKEVQHGTNSVEPIID
                     ALQEFRVQSTNYSAEFGTEAGGQINAVMKSGTNQFHGTAWEFLRNDRLDANNFFNNRT
                     GTERPAFRRNQFGVSGGAPLLLPKYDGRNRTFLFGAYEGTRVRKGITQLTTVPSAELR
                     SGNLSSLPTVRDPLTGQPFPNNTIPSNRINPIMAAILERYVPLPNRTGVFNWISTDPQ
                     RIDVEQYNWRIDHRFSETDSIFGHYIFEDTDFRYPKLFPTDGASQKFRGQNALISWTH
                     LMGANSVNDFRVGFSRFIQNEFQARAGRVNVVRELGMEGLCEIPSCWGIPQMNVTGFA
                     SFGEHGGQSVSGPRAWRNEAYQWQDSFYHTRGSHSIKFGVIVRRHRDNFPEAIYPRGA
                     YSFNGFLTGQPFGDFLLGYPRNTQTSIDVFSPHFRYTVAEPWFQDDWRVTPELTLNLG
                     LRYEWAGRPLSKDNSISTVIFDESGARLITARDPQGYPSALAYDDFNNFAPRIGFAWT
                     PKFLGSKTVFRGAYGIFYQRELANTWVDLAINDPFIRQTNINLETDASSPYHFARYDL
                     SRPTALAPPTPLLTFSVDTDWKDGQVHQWNFNIQQSLGFDTVLQVAYVGNRGLRLPWA
                     TLPNQAAPGPGPIQPRRPYPDFGVVNGLGSRGDSNYHGLQIQGEKRYSKGLLFMAGYT
                     FAKCISNSDSTFVGEGTSIQNGRDFSQQRGLCTQHFSHRYTLSWLYDLPFGRGKAFLG
                     NAPRPVEAILGNWQINGILTLRSGSPFTVGQPGDAPNTGEGSPRPDQVGDANDIEERT
                     VDRFFNTDAFAQADAFRWGTAGRNTVIGPGINNWDFSVFKSFAIDEARRIQFRAEFFN
                     LFNHAEFGFPGASFGTAQFGRISGTSRDPRDIQLSLKFLW"
     gene            complement(3900..4673)
                     /locus_tag="VFQ79_01500"
     CDS             complement(3900..4673)
                     /locus_tag="VFQ79_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138348.1"
                     /translation="MIDINAYLGHFAFRQLRHNTAPELLRLMDSRKIRQAAVSSADAI
                     AYRNVQPANEHLAAEVERHRDRLIPFAVINPTYAGWADDLATCQEKLGMKGLRLYPRW
                     HNYKLTDAACLELVKAATERGMLITIPVRVEDPRQRTWLVEIPDVPLAEIEGLVRAAP
                     SAQFAILNGLGYTGSALGRKGSGLPANYSIDVGRMSAVLANELGRLVENLGADRILFG
                     SGMPFQYPDPAIVKVEVLEASAADKEKILTRNAARLLRL"
     gene            complement(4670..5539)
                     /locus_tag="VFQ79_01505"
     CDS             complement(4670..5539)
                     /locus_tag="VFQ79_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138349.1"
                     /translation="MSFDALSRRAFLERAGAAGMAPVLGAQTRPAASTKTIDCHAHLY
                     PQNSAASRDSGRQLVEAADKLGIDQLCCSMLTARRPATADQFRECNRQVAESTKRFPG
                     RILGYVYVNPGYTKDALDDIRRCVEERGFMGVKLYNEYWIDEPVLNPLVELTIKLRIP
                     ILTHAGHMHYYLADQPRISDGSHIAKVASRYPEATIICGHVGGGGDWEWEIKSLRNAR
                     TVYLDTSGSVIDEGMIEMAVATLGADRVVFGCDMSMTAGVGKMRSARISESDRAKIMG
                     GNMAKIMARRQRS"
     gene            complement(5569..6474)
                     /locus_tag="VFQ79_01510"
     CDS             complement(5569..6474)
                     /locus_tag="VFQ79_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138350.1"
                     /translation="MKTPRRTFLKSAAALPAVVPAASASPALLPAIPLGKFQVTRLII
                     GANPFYGYSHFNRIFDDVMREWSTTENVCQTLSRCEQNGINTWQFSYNQRSMADLERH
                     RAEGGRLQWILLSSRELHEDPELIKRLAKAGPIGIVHHGGVTDNLCRRREFDKIRTFL
                     KQVRDSGAMVGMSSHTPANIARAEDEGWDTDFYMTCCYQVTRTADEVRKITGELPLPA
                     GEVYLEGDPARMFKVVRQTKKPCLAFKILAAGRRINNAEDLDRAFQFTFDSIKPNDAV
                     IVGMFPKFKDEVTDNAGRVRRILRA"
     gene            complement(6613..10050)
                     /locus_tag="VFQ79_01515"
     CDS             complement(6613..10050)
                     /locus_tag="VFQ79_01515"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0138351.1"
                     /translation="MAYQRAGVFAFMLAFLGLCFAQVETSTSIRGLITDPTGAAVPNA
                     RVAIRNVDTGEERNAVSNAGGSYAFPSVVPGTYDVTVTHPGFKRSEVKNRVAQVTQTA
                     QVDFRLEIGETTESVTVVAAGAELLSTSTAEVAGTIGTKLVEDLPLNGRNFFDLATTL
                     PHVSLQNIAPTASFAGFSMNAVLGSNPGNPLFRSSGIFAAGNRDSATNVSIDGVNVQS
                     SVYRQTTPQTPPSAIQEMKIHVSSMNAEFGNGVAAVNVITRSGTNRFHGEVYEYLRNE
                     KLDANYFFNNLASRGKNPFRQNQFGGALGGPLISNKLFFFGAYEGLRVRQSTLSIVTV
                     PPNDIRQGDFSQFRPAGPNNTFLPTPTIYNPYRYNPLTGLREPFPGNRIPLGATTLCA
                     PRPTCVDPVTQKFLENYVLAPNTVIDGIPRYIGDSRQRIDSDQGVLRMDWAKSEADRI
                     YGRYSRTVAPTINQPVESLAGLSQNASDQSAVVHWTRILSTNTVNDFMVGYARPKWLY
                     GKDLNAANASAEIGLLNTSQLAGGPSFGGTGYSMNSTLSFLLDGTDNLYQIGDDITHI
                     RGRHNLKFGFQTIERRFYYDNQSNDKGFFNFTPVFTSACPDGNTACATARNALGLANG
                     GNAFATFLLGTPLNGLFQLNNAAYRGHKRYYGVYAQDSWRVTSKLTLNYGLRYEYWSP
                     WLVPRHNVASFDEVNGNILYVLQNPLDYLNPETDFGKDAPLNSNIPREGHRTSKMNLA
                     PRIGLAYSVTGSTVFRAGYGIYYDGNSNTNQFSDISSAVAPFKLRYEPVISSSEQVPS
                     LQVQGNFPFPEPTAIPRPNSTPLGTFRFVPGYYPVSSVQEWSASIQQRFGSTLAAEIS
                     YQGTHAYHLPQFVDVNAPALPQGPLAGVPINERRPFPQWGVIGTWKPIGYGNYHGLGA
                     SLRGNYWKGLVFNSSFTFAKNIVSAMLGPSDTGNQHGAYPYIWEGPAQLTPRLRFVNA
                     ISYDLPFGPGRGMWTTGAAGFIAGGWTISTIADMTTGAPNRVTTNDLSGTGYGIMPNR
                     VCDPRDVPGGRNRFQWFNAACFEQPAFGTFGNSPIGVFEDPGINNWNFSLEKTTKTGF
                     PAETGALVFRMDIFNAFNHTQWGPASNSTVQSGNVNSGRVGSTRPPRQIQFSLAFQF"
     gene            complement(10178..11272)
                     /locus_tag="VFQ79_01520"
     CDS             complement(10178..11272)
                     /locus_tag="VFQ79_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0138352.1"
                     /translation="MPVIPSSVSTCTMTQLRKSRCTIRVRRSVIFIARECPEYQSWNR
                     ASNGIFAMSPSICYIDQHTCMDFRQLEMFLAVADNSSFTLAGKQLHVAQSAISRKVKM
                     LEQELGECLFKRVNKRIYLTPAGEIMLRYSRKVFQDLRNASLEVSEIGQMKRGTVRIG
                     AGMTACMHLLPPVLEKFRALYPNIETQVITGTAEALLYQLRNNLLDLGVLTMPVKQAD
                     LQTIPFYTEEMVVAVSPKHPVLSKRRSIAVRDLANYPMILFNRGTATRELLDRFFEQA
                     GIVPQVAMESESVATIAPLVQIDLGISILPIRVVLGPAKRKELHFLRLSDVRLVRQLG
                     LVFQKSDYQPRTIRELISLFCSSEQALLNG"
     gene            11178..12356
                     /locus_tag="VFQ79_01525"
     CDS             11178..12356
                     /locus_tag="VFQ79_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0138353.1"
                     /translation="MKITDLRTRIVHLDFRNCVIVQVDTDEGITGISETVMKRKTLTI
                     EQSILQLKPYLIGKDPTEIETHWESMYRDSFWVGGPMHATAISAIDCALWDILGRMCG
                     VPVHKLLGGPTRRKVPVYCHCPGGSSPSEFARHALSCVERGYRNIKTTLPLFYGASNS
                     DSAAYSGTNGSISRTWKETEHLSPGIFRRIREFFAAAREAVGPEIGLAVDCHGRLPLK
                     GAFRLCDALEDLDMLFIEEPVPPENVESFVKIQQHTSIPIAGGERWATIHGAREFIER
                     EAVDILQSDLVNCGGITGLKKMAAMAEAHSITMAPHNPNGPIATVMNLHFAAAVPNYF
                     MLETIGSDSDAKLWQDLIGWPLRLDDGYMQVPCEPGFGVELDEDAAALHPYVPHEGWR
                     "
     gene            12340..13578
                     /locus_tag="VFQ79_01530"
     CDS             12340..13578
                     /locus_tag="VFQ79_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0138354.1"
                     /translation="MRAGDKFLSTGVPKRRVAPGTWWICFLLLFATVLNYLDRQVLSL
                     TADKIIQEFHLTREQFGELLAWFRYSYAAFQIGGGWLVDRMGPRLLFPSAVGLWSAAG
                     ALTAFAGSMGTLAACRFMLGVGEAFNWPCALKTTQRLLPPEDRPLANGIFNSGTALGA
                     LLAPVVVTLLTFAFGWRSSFLLTGLLGGVWVVVWLYYTKSRTAELAGEQTSIRAVPGI
                     MLRIAMMPRFWLLAVSAVVVNGVSYFLADWIPLYLKVERGFTFAAGNFLSMLVYAGLD
                     AGNITSGLLVRRMAKRGVSVAKARKYTLFISCICMSCALAAGTASNIVTLAAIVITAV
                     GVAGFLVIYLTLVQELAPAHVGVSAGMLGGLGNLAYGLIAPSIGRFSDMQQTTTTFLL
                     IATLPWLAFACIAAGRWDDK"
     gene            13604..14572
                     /locus_tag="VFQ79_01535"
     CDS             13604..14572
                     /locus_tag="VFQ79_01535"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0138355.1"
                     /translation="MPYPLERFRGIFPAALTMFDRDNNLDEKATAAHWEWLIGQGVDG
                     LVIAGTSGEFIALENHERLRLFHLAKEVVQGRVPLIFGTGHYSTKLTIEMSQAAGELG
                     ADAIIVILPYYQKPYKSAVIRHFRDVRAAVDLPVMLYNNPANSACVDLTPREVVQLVD
                     EDVLHMVKSTYESVVPVHDLSYLAGGRMAIFYGSFQAAFEALAAGAHGWISGILNIAP
                     RAAKQMYAACAANDAARGFRIWKRILPLVHLYTHKLIGEVSDLATYRSILNMWGLNGG
                     YSRNPFYPLDAKQEEVLRRLLEDSGWLDPEHALEGMEEPMLERSAG"
     gene            14569..15468
                     /locus_tag="VFQ79_01540"
     CDS             14569..15468
                     /locus_tag="VFQ79_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138356.1"
                     /translation="MKRIAILGYGAVARLHAAELRKSGGVTLHSVFGPNTAKARDFAE
                     AFDIPHATGDLAAALEGADAAIVCSPSPRHFEQAREAVERVRAVLVELPACGSVVEAR
                     ALAQAASQRGTALHCAHTSRYLTPYRLISEWIRERLPGPVLQVRYARSVPPRQHSWVD
                     DALLHHAEHPLDLFLLWFGDVRPLGSALSPKTGPPQDAALLAELPSGAPLSVSISYRS
                     RIGGILMTIIGENHTVETDGFSFIRSDCAAWNQSFDGQQEYESAIGEQDKAFLSGQGI
                     PWIETMRLTSVVERFRVLARGVA"
     gene            15465..>19039
                     /locus_tag="VFQ79_01545"
     CDS             15465..>19039
                     /locus_tag="VFQ79_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0138357.1"
                     /translation="MTRRMCWIVAGLVLFRCGMSASGIPDFLPGTTPVAVPTDWREQQ
                     RLQMIRFLEQRTESAVKLRDEAWSRGGAETHRENLHALLGLHSSARAGAQAATLAEGT
                     IRIDDVVVDRQDGLRARALVFSNTGGNAANRSAVIAIPDSDQTAEAFAGIAPGGTPAK
                     WLLRLLAANAVVAVPTMVERSADYPLSHKLKNKDRRHILHRLGFVVGRSVTGLDVEKV
                     LLLREELASRNGIDRSRIVVMGAGQGGMTALYAAAAEPSFAGAAVLDYFNQREQAWSE
                     PVDRMFFGQLLEFGDAELAALVAPRKLLLGYRSGSTLLETAAPELARAQAYYQKANGR
                     DALSVMKNEADPLLQAAMYLVATDSAGTPGELSFRVPPERVQAARDGDFESLHAYLLW
                     LVEESEGRREEYWRKPQPARMREELARHEGVPQEPSAPLRPRTRLVRVTDKWTGYEVL
                     LDVFGGLEVYGHLLVPRGANKGLPAVICQHGLDGQPKDITGIGEKPGSPYHLFGAALA
                     DRGYVVFAPYVTVPIPQGKLINELVRRSAPLGMMRTGIEVRKLRAVVDFLSAQPFVDA
                     KRIGYYGLSYGGYSTLWMGPLEPRLAAIVISGHFNDWRSKITNHELATSYLMHPDEDF
                     YSWNALHRFTHPELIAAMSPRPVMVEFAEKDGTTTPEWHQRAWKQVEAMSSVLGVADR
                     VEREWFLGVHEIGGMRTFEFLDKWLRPEVSSSRDYKYLLWPTTRDLPGLGDNAADTWP
                     FIAHKLDSSDEAALRDTFRLSGPGDFQGLELRLSRAGHPGDVIVRFGSAPGASDVGEA
                     RIASKDVTPLYDLWHAVRIPPRKLVVGREYHVEVRAEGGRIPGDYYLVYAPKPLGGSP
                     RPSRFPFAYRPLETGEKDKLEEPTHEFVRQYLKPKLTAVELAPGASQNSDEAVLTSRW
                     TVQRPAQPDEIVETAAAELERFLKTIPSQMESAGVIDLRISNTGPEGVHSREGYRIDT
                     ASGRVTIEARDSRGIMRAIYWLEDAMLSRQGPFLKLGVTVRNERFPRRITTSITPGGE
                     RYTETSRPLLYTDGLLQRISRDGFNAIWIWLNTEEAAWNSRIFPELDDPEAHTRLARL
                     QDVARRAKRFGIDVYIYLATGYNHHIPEWFYKKYPDLKGYGWGSPMETTIPRIREYHA
                     EIVGNIFRHAPDLKGMVAIYDSEGFYHSGNREASRKQCRTCQNMTL"
BASE COUNT         3788 a         5583 c         5727 g         3941 t
ORIGIN      
        1 aaccgctacg gcagcgaact tgccacggcg gtcacggaga agatatccgg cgatctgcgc
       61 gccatctccg gccctttgcg cacggctttt tacattgaag agctggactt cgcaacgcct
      121 cccacgcgcg acgagctgaa gaagaaactg tcgcacgaga acgtgtacga acgccgccac
      181 gcggaagaga tgctgaagat catggaccgc gatggacggc tccctgcgaa atactcctac
      241 ccgctccagg tctggcagtt tggtccgaac ctcacgctga tcgcgatggc gggcgaagtg
      301 gtggtcgatt acaacctgcg gttgcggaag gaactcggcg aggaaaacac ctgggtggcc
      361 ggatacagca atgacgtttc cgcttacatc ccatccctgc gcatcctcaa agagggcggg
      421 tacgaaggcg gcggagccat gatctactat gtccagcccg gccccttcgc acccaatgtg
      481 gaagaactga ttatggagcg catccacaaa ctcgtccggc aggtgcgccc ggcgaagtga
      541 tccgcactac cataggaact tcagcgatag ctgaatgtct ctcgggtccc ggctggtgcc
      601 ggagatccgc ccgaattgcg ccgtgccgaa cgacgcgccc gggaaaccga actctgcgtg
      661 gttgaacaga ttgaagaact ccgcgcggaa ttgaatacgg cgcgcttcgt caatcgcaaa
      721 actcttgaac accgaaaagt cccagttgtt gatgccgggt ccgatcaccg tgttccggcc
      781 tgccgtgccc catcggaagg catccgcctg cgcgaaggcg tccgtgttga agaaacggtc
      841 cacggtccgc tcctcaatat cgttcgcatc gcccacctgg tcgggccgtg ggctgccctc
      901 tcccgtattc ggagcgtcgc ccggttgccc aacggtgaat ggcgaacccg accgcagcgt
      961 gaggatcccg ttaatctgcc agttgccgag aatcgcctcc acggggcgtg gggcgttccc
     1021 gagaaaggct ttcccgcggc cgaacggcag gtcatagagc cagctcagcg tgtaccggtg
     1081 gctgaagtgc tgggtgcaaa gtccgcgctg ctggctgaaa tcccgtccgt tctgaatgga
     1141 tgttccttcg ccgacaaacg tcgagtcgct gttgctgatg cactttgcaa aagtgtaacc
     1201 agccatgaac agcagaccct tggaataccg cttttctccc tgaatctgga gtccgtgata
     1261 gttcgaatct ccacgcgagc caagcccgtt cacaacgccg aaatcgggat aaggccgcct
     1321 gggctgtatc ggtccagggc ccggcgcggc ctggttcggc agcgtcgccc acggcagccg
     1381 cagaccccgg ttgcccacgt aagcaacctg caataccgtg tcgaacccca gagactgctg
     1441 gatgttgaag ttccactggt gaacctgtcc gtccttccag tcggtatcca ccgaaaaagt
     1501 caaaagcgga gtcggaggcg cgagcgccgt cggccggctc aggtcgtagc gcgcgaagtg
     1561 ataggggctg ctcgcatcgg tctccaagtt gatgttcgtc tgacgaatga atgggtcgtt
     1621 gatcgcgaga tccacccacg tgttcgccag ttcgcgctgg tagaagatcc catatgcgcc
     1681 gcgaaacacc gttttggatc cgaggaactt cggcgtccag gcgaagccga tgcgcggcgc
     1741 gaagttgttg aaatcgtcgt aggccagcgc gctgggatat ccctgcgggt cgcgggccgt
     1801 aatcagccgc gcaccgctct cgtcgaaaat gaccgtcgag atcgagttgt ccttggacag
     1861 tggccggccg gcccattcgt atcgcagacc caggttcaag gtcagctcag gcgtgacgcg
     1921 ccagtcgtcc tggaaccagg gctcggccac ggtgtagcgg aagtgcggtg agaacacgtc
     1981 gatgcttgtc tgcgtgttgc gcgggtagcc gagcaaaaag tctccgaacg gttggcccgt
     2041 cagaaagccg ttgaagctat aggcccctct tgggtaaatg gcctccggga aattgtcccg
     2101 gtgccgccgc acaattactc cgaacttgat gctgtgagat ccccgggtgt gatagaagct
     2161 gtcctgccat tgataggctt catttcgcca cgcgcgcgga ccggatacgg attgtccgcc
     2221 gtgttctcca aaagacgcga atcccgtcac gttcatttgt ggaatgcccc agcacgacgg
     2281 aatctcgcac agcccctcca tgccaagttc ccggacgacg ttcactctgc ccgcgcgcgc
     2341 ctgaaactcg ttctggatga aacggctgaa gcccacacgg aaatcgttca ccgagttggc
     2401 gcccatcagg tgcgtccagg aaatgagcgc attctggccg cgaaattttt gggaagcccc
     2461 gtccgtcggg aatagtttgg gataccggaa atcggtgtcc tcgaagatgt aatgcccgaa
     2521 aatcgaatcg gtctcggaga agcggtgatc gatgcgccag ttgtactgct cgacgtcgat
     2581 gcgttgcgga tcggtcgaga tccagttgaa cactccggtg cggtttggaa gcggcacata
     2641 ccgctccagg atggctgcca tgatcgggtt gatgcggttt gaagggatcg tgttattcgg
     2701 gaacggctgc ccggtgagcg gatctctcac cgtagggagg ctgctcaggt tgccgctgcg
     2761 aagctcagcc gaaggaaccg tggtcagttg cgtaatgccc ttgcggacgc gtgtgccttc
     2821 gtaggcgccg aataggaagg tgcggttccg tccgtcatac ttcggcagca ggagaggcgc
     2881 tccgcccgag acgccgaact gattccgccg gaacgccggc cgctctgtgc ccgtgcgatt
     2941 gttgaagaag ttgtttgcgt ccaggcgatc atttcggaga aactcccagg ctgtaccgtg
     3001 aaattgattg gtgccggatt tcataacggc gttgatctga ccgcctgcct ccgtcccgaa
     3061 ttcggcggaa tagtttgtgc tttgcacgcg aaattcctgg agcgcgtcga taatcggctc
     3121 cactgaattt gtcccgtgct gcacctcttt ggccatgaag ccattgatca gaaattggtt
     3181 cgcggtctgc cgctggccat tcacgctgaa ggcggtcccg gccgcgcgct caccataatg
     3241 gccggccacg gtcccaccgg agcgctcggt cgaccccgcc atcagcacca tcaactgtgt
     3301 gaagtttcgg ccattcagcg gcagatcggt aatggctttg gtctctacaa ccgcaccgat
     3361 ttgcgaatcc gccgtttgca attggggtgc cgaagaggtc acctgaacgg tctcggtaac
     3421 agcgccgatt tcgagcgcga agtccatttg cacgcgctgt gccacctcta ctcggacgct
     3481 gggcacgatc gcacgcttga accccggcgc ttcggcagta atcgtgtaga tccccaccgg
     3541 gacgatggcg aacgaataca gcccgtcact accagctatc gcggaccgtg tattgccggt
     3601 gccttcctgc tcgagcgtga cctttacatt tggaaccgtc gccccttggg ggtcgacaac
     3661 acgtccctgt atggaccccg cctcctgcgc ccccaatgga atcgtgagaa aggcggcgat
     3721 gagagtcgcg cccctgaaca ctgtggaaac actgcacatt tcatgccccc ctgtgagctt
     3781 gaagccgacg gctcaaaatg ccggacagca atccatttgc tgtccatagt caggggaaaa
     3841 tgcgcgtctg tcaatagaaa atgcgagatg cgtgcgccca aaatacgaac aattcgaagt
     3901 tacaggcgta acaagcgggc cgcgttacgc gttaagatct tttccttatc cgcggcgctg
     3961 gcctccagta cttctacctt cacaatcgcc ggatcaggat actggaaggg catgccactg
     4021 ccgaacagga tgcggtctgc gcccaggttc tccaccagcc tgccgagctc attggccagc
     4081 acggccgaca tccgtccgac atcgatcgag tagtttgccg gtagccctga acctttgcgg
     4141 cccaacgcgg agccggtata ccccagcccg ttgagaatgg cgaactgtgc tgagggcgct
     4201 gcccgcacca gcccctcaat ctcggccagc ggtacgtccg gtatttcgac aagccaggtc
     4261 cgctgccgtg ggtcctccac ccggaccgga atggtgatga gcatgccccg ctcggtggct
     4321 gcctttacaa gttccaggca cgccgcgtcg gtgagcttgt agttgtgcca gcgcggatac
     4381 agccggagtc ccttcatccc gagcttttcc tggcaagtgg cgagatcgtc cgcccatccc
     4441 gcgtacgtgg gattgatgac cgcgaacggg atgaggcggt cgcgatggcg ctccacttcg
     4501 gcagccaggt gctcgttggc gggctgcacg ttccggtatg cgatggcatc agcgctcgaa
     4561 acagcggcct gccggatctt gcggctgtcc atcaaccgca gcagctcagg agccgtattg
     4621 tggcgaagct ggcgaaaagc gaagtgacct aagtacgcgt tgatatcgat catgaccgct
     4681 gtctcctcgc cataattttg gccatatttc cgcccatgat cttcgctctg tcggattcgc
     4741 tgatccgggc gctgcgcatt ttcccgacgc ccgccgtcat gctcatatcg catccgaaaa
     4801 caacgcgatc cgcgccgagt gttgctactg ccatttcgat catgccttca tcaatgacgc
     4861 tgccgctggt gtcgagatag accgttcgag cattacgcag ggacttgatc tcccattccc
     4921 aatcgccgcc accgccgacg tgcccgcaaa tgatcgtcgc ttccggatac cggctggcca
     4981 ctttcgcgat gtgcgagccg tcgctgatgc gcggctggtc cgccaggtag tagtgcatgt
     5041 gcccggcatg cgtaagaatg ggaatgcgca gcttgatcgt aagctccaca agcggattga
     5101 gaaccggctc gtcaatccaa tactcgttgt agagctttac gcccatgaag ccgcgttctt
     5161 cgacgcagcg ccgaatatcg tcgagcgcgt ctttcgtgta tccgggattg acgtagacat
     5221 aacccaggat ccggcccgga aatcgctttg tcgattcggc cacctgccgg ttgcactcgc
     5281 gaaactggtc tgccgtagcg ggacggcgcg ccgtcagcat cgaacagcag agttggtcga
     5341 ttccgagctt gtccgccgct tcgacaagct ggcggccgct gtcccgcgat gccgccgaat
     5401 tttgcggata aagatgcgca tggcagtcga tggtctttgt ggatgcggcc gggcgtgttt
     5461 gcgcgcccag tacaggcgcc atgcctgccg ctccggcacg ttccagaaac gcgcgtcgtg
     5521 agagggcatc aaatgacata aaagataatc tgtgcgaaca ggccgacgtc aagcgcggag
     5581 aatccgccgc acacggccgg cattgtccgt gacctcgtcc ttaaactttg gaaacattcc
     5641 gacgatcacc gcgtcgttcg gtttaatgct atcgaaggtg aactggaatg cgcgatccag
     5701 gtcttccgcg ttgttgatgc gccggccggc cgccagaatt ttgaaggcca ggcagggttt
     5761 ctttgtttgc cggactactt tgaacatgcg cgcagggtca ccctccaaat aaacctcacc
     5821 tgcgggtagg ggcaattcgc cggttatttt ccgcacctca tcggccgtcc gcgttacctg
     5881 gtagcagcag gtcatataga agtcggtgtc ccaaccttca tcttcagccc gcgcaatgtt
     5941 agccggtgtg tgagaagaca ttcccaccat cgctccgctg tccctcacct gcttgaggaa
     6001 cgtgcggatt ttatcgaatt cccggcggcg gcacaaatta tccgtcacac cgccatgatg
     6061 cacaattccg atgggacccg ctttcgcaag cctcttgatc aactccggat cttcgtgcag
     6121 ctcgcgggag ctcaagagaa tccactggag ccggccaccc tcggcgcggt ggcgttcaag
     6181 atcggccata ctgcgttgat tataggagaa ctgccacgtg ttgatgccat tctgctcgca
     6241 ccggctgaga gtttggcata cattttcggt ggtgctccac tcccgcatca catcgtcaaa
     6301 aatccggttg aaatgggagt atccataaaa cgggttcgca ccaatgatca gccgggtgac
     6361 ctggaatttg ccaagcggga tagcaggcag cagagcgggc gaagccgatg ccgcggggac
     6421 caccgccggc agtgccgcgg cggatttcag aaaagtgcgt cggggagtct tcatatgtac
     6481 tccggagtta cggattgcca actccactat ataaaagagt gaagtcactg gcaatcgtcc
     6541 cggagcaccg gcgccgggcg gacggtgagc gtccgcccgg tctccccggc gccagtacac
     6601 aggcggtggc tgctaaaact gaaacgcgag cgaaaactgg atctgccggg gcgggcgcgt
     6661 ggaaccgact cggcccgaat tgacgtttcc ggattgtacc gtgctattcg atgcgggacc
     6721 ccattgcgta tggttaaacg cattgaatat atccatccgg aaaacgagag caccggtctc
     6781 ggcggggaat ccggtcttgg tcgtcttctc gagcgaaaag ttccaattgt taattcccgg
     6841 atcttcaaag acgccgatcg gcgaattgcc aaaagtccca aaagcaggct gctcgaaaca
     6901 cgcggcgttg aaccattgga accggttgcg cccgccggga acgtcccgcg gatcacagac
     6961 acggttcggc ataataccgt aaccggtgcc ggagaggtcg ttggtcgtaa cgcggtttgg
     7021 cgccccggtc gtcatatcgg cgatcgtgct gatggtccag ccgcccgcaa tgaaaccagc
     7081 ggcaccggtc gtccacatac ctcgtccagg cccgaacggg agatcgtacg aaatggcatt
     7141 gacgaaacgc aagcgcggtg tgagttgggc aggcccttcc catatgtaag gataggcgcc
     7201 gtgctggttt ccggtatcgc tgggaccaag catcgcgctg acaatattct tcgcgaaggt
     7261 gaagctggag ttgaaaacga gccccttcca gtagttgccg cgcaggctgg cgcccaagcc
     7321 gtgatagttg ccgtacccga tgggtttcca cgttccgatg acgccccact gcgggaaggg
     7381 acgccgctcg ttgatcggca cgccggcgag aggcccttgc ggaagcgcgg gggcattcac
     7441 gtccacgaac tggggcaggt ggtaggcgtg agtgccctgg taggagatct ccgccgccag
     7501 cgttgacccg aacctctgtt gaatgctggc cgaccactcc tgcacgctgg agacaggata
     7561 gtatcccggg acaaaccgga atgtgccaag cggagtggag ttcggtcgcg gtattgccgt
     7621 aggttccggg aacggaaagt taccctgcac ctggagactt ggcacctgtt cgctcgatga
     7681 aataaccggc tcataccgca gcttgaacgg cgcgacagcc gagctgatgt ctgagaactg
     7741 gttggtgttg ctgttgccgt cgtagtaaat gccgtagccc gcccggaaca ccgtgctgcc
     7801 ggttacggaa tacgctaatc caattcgcgg agccaaattc atcttgctcg ttcgatgtcc
     7861 ctcgcgggga atgttggagt tcagcggggc atccttgccg aagtcggttt caggattcaa
     7921 ataatccagc ggattctgga gcacgtacag gatgttcccg ttcacttcat cgaagctcgc
     7981 gacattatgg cgcggaacca gccacgggct ccagtattcg tatcgcaagc catagttcag
     8041 cgtgagtttg cttgtgacgc gccaggaatc ctgggcatac acgccgtagt agcgcttgtg
     8101 cccgcggtaa gcagcgttgt tcaattggaa cagcccattg agcggcgttc ccagcagaaa
     8161 cgtggcgaac gcattcccgc cattggcgag gccgagagcg tttctggcgg tggcgcaggc
     8221 ggtattgcca tccggacagg ccgacgtaaa taccggcgtg aagttgaaaa agcctttgtc
     8281 gttcgactgg ttgtcgtaat aaaagcggcg ctcgatagtc tggaatccga atttcagatt
     8341 gtgccgtccg cggatgtgcg tgatgtcgtc tccaatctgg tagaggttat ccgtgccatc
     8401 cagcagaaag ctcagggtgc tgttcatcga ataccctgtt ccgccgaacg acggtccgcc
     8461 ggctagttgg gaggtattca gcaatccaat ctcagccgag gcattcgcag cattcaaatc
     8521 ttttccgtat agccatttcg ggcgggcata gcccaccatg aaatcgttca cggtgttcgt
     8581 cgatagaatc cgcgtccaat gcaccacggc gctttggtcg ctggcattct gcgacagacc
     8641 ggccagcgat tcgacaggct ggttgatggt cggtgcgacg gtcctggaat accggccgta
     8701 aatgcggtcc gcttcgctct ttgcccagtc catccgaagg actccctggt cgctgtcgat
     8761 ccgctgccgg gaatcgccaa tgtaacgcgg aatgccgtcg atcacggtgt tcggagcgag
     8821 cacatagttt tccaggaact tctgggtgac cgggtccacg caggtcggac gcggcgcgca
     8881 aagggtcgtg gctccgagcg gaatgcgatt gcccggaaat ggctcccgca agcctgttag
     8941 cggattgtag cgatacggat tgtaaatggt cggcgtgggg agaaacgtgt tattcggccc
     9001 ggcgggccgg aactgcgaaa aatcgccctg ccggatatcg ttcggcggaa ccgtgacgat
     9061 cgatagcgtc gactgccgga cccgcagccc ctcataggcg ccgaaaaaga acagcttgtt
     9121 tgaaatcagc ggtccaccta acgccccgcc gaactggttc tgacggaagg gattcttgcc
     9181 gcggcttgcg agattgttga agaaatagtt ggcgtccagt ttttcgttgc gcaaatactc
     9241 gtaaacctct ccatgaaacc ggttggttcc actccgcgta atgacgttga ccgcggctac
     9301 gccgttgccg aactcggcgt tcatgctgga gacgtgaatc ttcatttcct gaattgccga
     9361 gggcggcgtc tggggtgtcg tctgtcggta gacggagctt tgcacgttta ccccgtcgat
     9421 cgacacgttg gtcgccgaat cgcgattccc cgcggcgaaa atgccggagg aacggaacag
     9481 cggattgcct ggattcgacc ccagcaccgc gttcatggaa aaaccggcga acgaagccgt
     9541 cggcgcgatg ttctgaagcg atacgtgcgg tagcgtggtt gccaggtcga agaagttgcg
     9601 tccgttcagc ggcaggtcct ccaccagttt ggtgccgatg gtacccgcta cctccgcggt
     9661 gctcgtcgag aggagttccg cgcccgccgc gacgaccgtg accgattcgg tggtttctcc
     9721 aatctcgagc ctgaagtcta cctgcgcggt ctgtgttact tgggccacgc gattcttcac
     9781 ctcggacctt ttgaatcccg ggtgagtcac cgtcacatcg tacgttccag ggacgacgga
     9841 aggaaacgcg taagagcctc ccgcattgct gacagcattc cgttcttcgc ctgtatccac
     9901 atttcgtatt gccactctcg cgttcggaac ggcggcgccg gtcggatccg taatgagccc
     9961 gcgaatgctg gtggatgttt cgacctgcgc aaagcacagg cccaaaaagg cgagcataaa
    10021 tgcaaataca cctgcacgct ggtatgccat ggctagccct ctttccaatg cgaagtgacg
    10081 acccgggtgc gagccggttt tgtaaggaaa tattactggc agcgagaatc cgagtcaaat
    10141 atagaaattc gttcttttaa tctaatgtcg atatggatca gccattcaga agggcctgct
    10201 cactcgaaca aaacaggctg atcaactcgc ggatagtgcg cggctggtaa tcggatttct
    10261 ggaaaaccaa tcccagttgc cggaccagcc tgacatcgga aagccgtaga aaatgcaact
    10321 ccttgcgttt ggccggcccg agaaccaccc ggattggaag aatgctgatg ccgagatcga
    10381 tctgcacgag cggggcgatg gtggcgacac tttccgactc catggcaacc tgcggcacga
    10441 tgccggcttg ctcgaagaac cggtcgagca gttcgcgggt tgcggtgccc cggttgaaaa
    10501 ggatcatggg atagtttgcc aggtcgcgta ccgcgatgct ccggcgcttc gacaggaccg
    10561 gatgcttcgg gctgacggcc accaccattt cctcggtata aaaaggaatg gtctgaaggt
    10621 ccgcttgttt tacaggcatc gtcaaaacgc ccagatccag caggttgttg cgaagttgat
    10681 aaagtagcgc ttcggcggta ccggttatca cctgcgtctc gatattcgga tacagtgccc
    10741 ggaacttttc aagcaccggc ggcaaaaggt gcatacacgc ggtcatgccg gcgccgatgc
    10801 gtacggtacc gcgcttcatc tggccgattt cggagacttc gagcgatgcg ttgcgaagat
    10861 cctgaaacac ctttctcgaa taccgcagca tgatctcgcc cgcgggagta aggtagatgc
    10921 gcttgttgac ccgcttgaac agacactcgc cgagctcctg ctccagcatc ttgactttgc
    10981 ggctgattgc ggactgggcg acatgcaatt gctttccagc cagcgtgaag ctggaattgt
    11041 cggcgacggc cagaaacatt tcaagctgcc gaaaatccat acaggtgtgt tggtctatat
    11101 agcaaataga tggactcatt gcaaatattc catttgacgc acggttccat gactgatact
    11161 ctgggcactc gcgggcaatg aaaatcaccg atcttcggac gcggattgtg cacctggatt
    11221 tccgcaactg tgtcatcgtg caggtggaca ccgacgaagg tataaccggc atctccgaaa
    11281 cggtgatgaa gcgcaagacc ctcaccatcg agcagtcgat cctgcaactg aagccttatc
    11341 tcatcgggaa agatcccacc gaaatcgaga cccactggga aagcatgtac cgggattcgt
    11401 tctgggtcgg cggcccgatg catgccacgg cgatcagcgc gatcgattgc gcgttatggg
    11461 acattcttgg ccggatgtgc ggggttccgg tgcacaaact gcttggcgga cccacccggc
    11521 gaaaagttcc ggtttactgc cattgtcccg gcggatcgtc tccttccgag ttcgcgcggc
    11581 atgcgttatc gtgcgttgag aggggttacc ggaacatcaa aaccacgctt ccgctgttct
    11641 acggcgcaag caacagcgac tcggcggcat actcgggaac gaacggcagc atctcccgta
    11701 cctggaaaga aacggagcac ctttcaccag ggatttttcg ccgcatccgt gagttttttg
    11761 ccgctgctcg cgaggctgtt ggcccggaga ttggcctcgc ggtcgactgc cacggacggc
    11821 ttcccttgaa aggagctttc cggttatgcg atgcgctcga agatctcgac atgctcttca
    11881 tcgaagaacc ggtgccgccc gaaaatgtcg aatcgttcgt gaagatccaa cagcacactt
    11941 ccatccccat tgccggcggc gagcggtggg caactattca tggtgcgcgc gagttcatcg
    12001 agcgtgaagc cgtcgacatt ctgcaatccg atctcgtcaa ctgcggtggc atcacgggtc
    12061 tcaagaagat ggcggcgatg gccgaggcac acagtatcac gatggcgccg cacaatccga
    12121 acggacccat agcgaccgtc atgaatctgc acttcgccgc ggccgttcca aactatttca
    12181 tgctcgaaac gatcggctcg gacagcgacg caaagctgtg gcaggacctg atcggctggc
    12241 ctctgcggct ggacgacgga tacatgcagg ttccatgcga accaggcttc ggtgtcgagc
    12301 tcgacgaaga cgcggccgct ctccatcctt acgtacctca tgagggctgg cgataagttc
    12361 ctttccaccg gcgttcccaa gaggcgagtg gcgccgggca catggtggat ctgctttttg
    12421 ctgctcttcg ctaccgtcct gaactacctg gaccggcagg tgctctcgct caccgcggat
    12481 aaaatcatcc aggagttcca tctcacccgc gaacagttcg gcgaactgct ggcatggttt
    12541 cgttattcct acgcggcttt tcagatcggc ggcggatggc tcgtggacag gatgggcccg
    12601 cgcctgttgt ttccctccgc cgtgggacta tggtcggccg ccggcgccct gaccgctttc
    12661 gcaggctcca tgggcacgct ggcggcctgc cggttcatgt tgggtgtcgg cgaagctttc
    12721 aactggccct gcgcgctgaa gacgacccag cgccttcttc caccggaaga ccgccccctg
    12781 gcgaacggca ttttcaacag cggcaccgcg ctgggcgcgc tgctcgcgcc ggttgtcgtg
    12841 actcttctca cctttgcctt tgggtggcgc tcctcgttcc tgcttacggg gttgttggga
    12901 ggcgtgtggg tggtggtttg gctgtactat acgaaatcac gaacggccga gctcgcgggc
    12961 gaacagactt ctattcgcgc agtccctggc atcatgttgc gcatcgcgat gatgccccgc
    13021 ttctggctgc tggccgtttc cgcggtggtg gtgaacggcg tcagctactt tttggcggat
    13081 tggattccgc tctatctaaa ggtggaacgc ggcttcacgt tcgctgccgg aaattttctg
    13141 tcgatgctcg tctatgcggg gctcgacgcc ggcaatatta cttcgggcct gctcgtccgg
    13201 cgcatggcaa agcgcggtgt gagcgtagca aaagcccgca agtatacgct gtttatcagt
    13261 tgtatctgta tgtcatgcgc gcttgcggcc ggcacggctt cgaacatcgt aacgctggcc
    13321 gcgatcgtaa tcaccgctgt cggtgtagcg ggctttctgg tgatttactt gacgctggtg
    13381 caggagctcg ccccggcgca tgtcggtgtc agcgcaggca tgctcggcgg tttgggaaat
    13441 cttgcatacg gcttgattgc acccagcatt ggacgctttt ccgacatgca gcagacgacg
    13501 actacattcc tgctgatcgc cacgctgccc tggctggcct tcgcgtgtat cgcggccggc
    13561 aggtgggacg acaagtgaga cagccggact caggagaatc gaaatgcctt atccattaga
    13621 gcgcttccgc gggatcttcc ccgcggcgtt gacgatgttc gatcgcgaca acaacctgga
    13681 tgagaaggcc actgccgctc attgggagtg gctgatcggg cagggagtgg acggccttgt
    13741 catcgccggt accagcggcg aattcatcgc cctcgaaaat cacgagcgcc tgcgcctctt
    13801 ccaccttgct aaagaggttg tgcaaggccg cgttccactc atcttcggca caggccacta
    13861 cagcacgaaa ttgacaatcg agatgagcca ggccgccggc gaactgggag cggatgcgat
    13921 catcgtaatc cttccctatt accagaagcc gtacaagagc gccgtcatcc gccacttccg
    13981 ggatgtgcgc gccgcggtgg acctgccggt gatgctctac aacaatccgg cgaactcggc
    14041 gtgcgtcgac ctgacacccc gcgaagtcgt gcaactggta gacgaagacg ttctgcacat
    14101 ggtgaaatcc acctacgaat cggtggttcc cgtgcacgac ctcagctacc tggcgggcgg
    14161 ccgcatggcg atcttttacg gctcttttca agccgccttc gaagcgttgg ccgcgggagc
    14221 gcacggctgg atcagcggca tattgaacat cgcgcccaga gccgcgaagc agatgtatgc
    14281 cgcatgcgca gccaacgacg cagcgcgcgg ttttcgcatc tggaaacgca tcctgccgct
    14341 ggttcacctc tacacgcaca agctgatcgg cgaggtcagc gacctcgcga cctaccggtc
    14401 cattctgaac atgtggggct tgaacggtgg ttatagcagg aatccgtttt acccgctgga
    14461 tgcgaaacag gaagaggtac ttcggcggct tcttgaagat tcgggatggc tcgatcccga
    14521 acacgccctg gaaggcatgg aagagccgat gctggagcgg agcgccgggt gaagcggatc
    14581 gccattctgg gctatggcgc cgtcgcgcgg ttgcacgcgg ctgagttgcg caagagcggt
    14641 ggcgtgactc tccactccgt attcggtcct aatacggcca aggcgcggga ttttgccgag
    14701 gccttcgaca ttccgcacgc caccggcgat ctcgccgccg ctctcgaagg agccgatgct
    14761 gccatcgtct gttccccttc accgcgccac tttgagcagg cacgcgaagc tgttgagcgc
    14821 gttcgggccg tgctggtgga acttccggca tgcgggagtg tggtggaagc gcgggctctc
    14881 gcgcaagcgg cgtcgcagcg aggaaccgct cttcattgcg cacatacgag ccgctacctg
    14941 acgccttacc ggctcatctc cgaatggatt cgcgagcggc ttccgggccc ggtattgcag
    15001 gttcgttatg cgcgctcggt tcctcccagg cagcacagtt gggtggatga tgcattgctc
    15061 caccacgctg agcacccgct ggatctgttc ctgctctggt tcggcgatgt gcgtccactg
    15121 ggaagcgcgc tctcgccgaa aacgggacct ccgcaggacg ccgcgctgct ggcggaactt
    15181 ccgtcgggcg cgccgctatc ggtgagcatc tcgtatcgct cgcgaatcgg cggcatactc
    15241 atgacgatca tcggcgaaaa ccacacggtg gaaacagatg gattcagttt catccgctct
    15301 gattgcgccg cctggaatca atcgttcgat ggacaacagg aatacgaatc cgccataggg
    15361 gaacaggaca aagcgttcct ttcggggcag ggcattccct ggatcgaaac catgcgattg
    15421 acttcggtgg tcgaacgatt ccgcgtgctg gcaaggggtg ttgcatgacg cgaaggatgt
    15481 gttggattgt tgccgggctg gtgctcttcc gttgtgggat gtccgcttcc ggcattcccg
    15541 atttcctgcc tggaactact cctgtggcag tgcccacgga ctggcgcgag cagcaacggc
    15601 tccagatgat tcgttttctc gaacagcgca cggagtcggc ggtgaagctg cgcgatgaag
    15661 cctggagccg tggaggcgcg gaaacacacc gggaaaatct ccacgcgctg ctcggccttc
    15721 actcttctgc gcgggctggc gcgcaggcgg caacgctcgc ggaaggcacc atacggatcg
    15781 atgatgttgt cgtcgacagg caggacgggc tgagagctcg cgcgctcgtt ttctcgaaca
    15841 ccggcggcaa cgccgcgaat cgctccgccg tcatcgccat tccggacagc gatcagaccg
    15901 ccgaagcctt cgctggaatc gcacccggcg gcacaccggc gaaatggctt ctgcggctgc
    15961 tcgccgcgaa tgcggttgtg gctgttccca ccatggtgga acgaagcgcc gattatcctc
    16021 tcagccacaa gctgaaaaac aaagaccggc ggcacatcct gcaccggctc ggattcgtgg
    16081 ttgggcgaag cgtgaccggc ctagacgtag agaaagtttt gctgcttcgt gaagagctgg
    16141 cgagccggaa cgggatcgac cgttcgcgga ttgttgtgat gggtgcgggc cagggcggca
    16201 tgaccgccct ctatgccgcg gcagccgagc cgtctttcgc cggagcggcc gtgctcgatt
    16261 atttcaatca gcgcgaacag gcctggagcg aaccggtaga ccgcatgttc tttggccaac
    16321 tgctggagtt tggagatgcc gagctggcgg cgctggttgc gccgcgaaaa ttgctgttgg
    16381 gttatcgcag tgggagcacg cttctcgaaa ctgcggcgcc ggaactggcc cgcgcccagg
    16441 catactacca gaaagcgaac ggcagagatg cgctgtctgt catgaagaat gaagccgatc
    16501 ctttgctgca ggcagcgatg taccttgtag caacggactc cgccggtacg cctggagagt
    16561 tgtcattccg cgtgccgcct gaacgcgtgc aagccgctcg cgacggggac ttcgaatctc
    16621 tgcacgccta ccttttgtgg ctggtcgagg agagcgaagg caggcgtgaa gaatattggc
    16681 gtaagccgca acccgcgcgc atgagggaag aactggcgcg gcacgaaggt gtgccacagg
    16741 agccttccgc cccgctgcgc ccgcgtacgc gcctcgttcg ggttacggac aagtggaccg
    16801 gttatgaagt gctgctcgat gtgttcggcg gactggaagt gtacggacat ttgcttgtgc
    16861 cccgcggagc aaacaagggc cttccggcag tgatctgcca acatgggctc gatgggcagc
    16921 cgaaagacat caccggtatt ggagagaagc cggggtcacc ctatcacctg ttcggagccg
    16981 ctctggcgga tcgcggatat gtggtgttcg cgccctatgt gacggtccct attccacaag
    17041 gcaagctgat caatgaactg gtgcggcgct ccgcgcccct cggcatgatg cgcaccggca
    17101 tcgaggttcg aaagctccgg gccgtagtcg attttctctc ggcacagcca ttcgtcgacg
    17161 cgaaacgaat cggctattac gggctgtcct atggaggtta ctcgacgctg tggatgggtc
    17221 ctctcgaacc ccggctcgcc gccatcgtca tctccgggca cttcaacgac tggcgttcga
    17281 agatcaccaa tcacgaactt gccaccagct atctgatgca tccggacgaa gacttctata
    17341 gctggaatgc gctccatcga ttcacacatc ccgagttgat tgctgcgatg tcgcctcgtc
    17401 cggttatggt cgagtttgct gaaaaagacg gcacaacgac gcccgaatgg caccagcgcg
    17461 cgtggaagca ggtggaggca atgagctcgg ttttgggagt agccgaccgc gtcgagcggg
    17521 agtggtttct tggcgttcac gaaatcggag gcatgcgcac gttcgagttt ctcgacaaat
    17581 ggttgcgtcc ggaggtctcg tcttcgcggg actacaagta tctgctctgg ccgaccacgc
    17641 gcgatctgcc cggattggga gataatgcgg cggacacctg gccgtttatc gcgcacaaac
    17701 tggattcgag cgacgaagcg gcgcttcggg ataccttccg cctttccggc ccgggcgatt
    17761 tccagggctt ggagcttcgt ctctctcgcg ccggacatcc aggcgacgtc attgtgcggt
    17821 tcggctccgc gccgggcgcg tcggacgtgg gcgaagctcg tatcgcgagc aaggacgtca
    17881 caccgcttta cgacctttgg catgccgtcc ggattcctcc caggaaactg gtcgtgggac
    17941 gcgaatacca cgttgaggta cgcgcagaag gcggccggat tccgggcgac tattatcttg
    18001 tctatgctcc gaagcctttg ggtggcagtc cgcggccaag tcggttccct tttgcatacc
    18061 ggccgctcga aaccggcgag aaggacaaat tggaagagcc cacgcacgag ttcgtacgcc
    18121 aatacctgaa gccgaaactc acagcagtag agcttgcgcc cggagcttct caaaattctg
    18181 atgaagcggt tctcacgtcg cgatggactg tgcagcggcc cgcgcaaccg gacgagatcg
    18241 tcgagacggc tgccgcagaa ctcgagcgtt tcctgaaaac gattccatcg cagatggaaa
    18301 gtgcgggggt gatcgatctg cggatttcga acaccggacc ggaaggcgta cactcgcgcg
    18361 agggttatcg catcgatacg gcgtccggcc gcgtaaccat cgaagcccgc gactcccgcg
    18421 gaatcatgcg ggccatctat tggctcgaag acgccatgct gtcgcgccag gggccttttt
    18481 taaagcttgg tgtgaccgtg cggaacgagc gctttccacg ccgcatcaca acttccatca
    18541 caccgggcgg cgagcgttat accgaaacct cccgtccgct gctttacacg gacggactcc
    18601 tgcaacgtat ttcgcgagat ggattcaatg cgatctggat ctggctcaat actgaagagg
    18661 cggcatggaa ttccaggatc ttcccggaac tggacgatcc ggaagcgcac accaggctcg
    18721 cgcgtctcca ggacgtcgcg cgccgcgcca aacgcttcgg catcgatgtc tacatctacc
    18781 tggcgaccgg atacaaccat cacatcccgg aatggtttta caagaagtat ccggacctca
    18841 agggctacgg ctggggttcg ccgatggaga ccaccatccc gcgcattcgc gaatatcacg
    18901 ccgagatcgt gggcaacatt ttccggcacg cgccggatct gaagggaatg gtcgccatct
    18961 acgacagtga aggcttctat cattcgggca atcgcgaggc gagccggaag caatgtagga
    19021 cgtgccagaa tatgacgct
//
LOCUS       DASTMK010000041        37239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_66546, whole genome shotgun sequence.
ACCESSION   DASTMK010000041 DASTMK010000000
VERSION     DASTMK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 37239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37239
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66546"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFQ79_01550"
     CDS             <1..483
                     /locus_tag="VFQ79_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU0138358.1"
                     /translation="YLGVPQQEIPKSFLFAGERTEGGAPYAVLHPFASTPEKTWPANR
                     FLEVARWVQSEWNLEPVFIGAPGEDLSAFSAYRTATASLSGTKTLIAGASLFLGNDSG
                     PAHMAAAFSIPLVVLFGPSDATIWGPWRAASETLVRQPMEDITSGEVVQALQRLRVSV
                     "
     gene            480..2309
                     /locus_tag="VFQ79_01555"
     CDS             480..2309
                     /locus_tag="VFQ79_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0138359.1"
                     /translation="MKQLLRILRYVKPYIPALLAAVLLMAAVGAMQALTALLIGPIFD
                     RVLDPSTVDAPVLLFTVPLLHKQIYLHQLAPSSFHNVWSTVAFGILATFLMKGLCDYA
                     GNYLINYVGFSAVTDLRQRVFDSVLRQDAQFFELHSTGRLMSSIMNDIEKIQVATSHI
                     LADWLRQSFSAVFLTIVVLQRDWRLAVASLTVLPFVLVPTLRLGRRLRRTTRRAQDDA
                     AELNQILQETLSGHQVVKSFGAEAEESSRFRAAAKKFRSSNLRYVAQQAIASPLIEFF
                     GAVTIVGLLTYARTQIKNGDMTTGEFTSFVIALFMLYEPVKRLTGIHNIFQQALGASQ
                     KVFGYLDRPVAVHERTDARVLKRFTESVVFEDISFRYPNSPNGFLLKKVHLDVKAGEV
                     VALVGPSGAGKTTLANLLPRFYDVTSGTVRIDGIDVRDLTLKSLREQIGIVAQDTFLF
                     NDTVANNIRYGRPGASRAEVEEAARNALAEEFILRLPEGYESWIGERGLKLSGGQRQR
                     IAIARALLKNAPILILDEATSHLDTESEILVQKALSNLMKDRTVIVIAHRLSTIRRAD
                     KIVVLDGGQISETGSHDELVSGGGIYQRLHELQFLEADPMVNP"
     gene            2306..3196
                     /locus_tag="VFQ79_01560"
     CDS             2306..3196
                     /locus_tag="VFQ79_01560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU0138360.1"
                     /translation="MIRSMTGFARARKTTEDGEIVLSVKSLNHRGLDLHFHLPVVFDA
                     YENAMREALKHKVARGHVDIRVVWHPVRSTGSAGLNRPLLDTYIAGFREAARQYGIAG
                     EPDLNAALRVPGMFSDTDDAEVSPGVQAVLMAALAEALEELNAFRAREGAQLASLMLE
                     RNQAIASAAERLETLRLGAVQAFENRLTERLAELLRGVAVEPQRLAQEAAILADRSDI
                     GEEIARLKIHSGQLQQLLEGGTETGKKIDFLLQEMNRETNTILSKSTGLGEMGMEITN
                     LGLAVKSDIEKIREQALNLE"
     gene            3193..3807
                     /gene="gmk"
                     /locus_tag="VFQ79_01565"
     CDS             3193..3807
                     /gene="gmk"
                     /locus_tag="VFQ79_01565"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU0138361.1"
                     /translation="MTTVFIISAPSGSGKSTLVSRLLQRDPQLVFSVSFTTRPPRGVE
                     LEGKDYHYISREEFEKRLLHSEFLEHAEVFGNYYGTHRSILDKARSQGKDLVLDIDVQ
                     GARQLKDRLPDAVSIFILAPSRQELEERLRSRSEDSQEVIERRLRDAAEEIRKYRQYD
                     YIVINRQVETSVNTLAAIIRAERARRSRMEDEIRPILETFETYK"
     gene            3820..4068
                     /gene="rpoZ"
                     /locus_tag="VFQ79_01570"
     CDS             3820..4068
                     /gene="rpoZ"
                     /locus_tag="VFQ79_01570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU0138362.1"
                     /translation="MPEKLTRNQSIHSIPDDTEQSAYRFIILSAKRARQLQNGARSFL
                     PTTSRKPTITAMEEVRRGLVKYSDTGRDAELNPVAEES"
     gene            4081..5289
                     /gene="coaBC"
                     /locus_tag="VFQ79_01575"
     CDS             4081..5289
                     /gene="coaBC"
                     /locus_tag="VFQ79_01575"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU0138363.1"
                     /translation="MNVILGVSGGIAAYKAAELARAFMERGHSVQVVMTAAAGEFIQP
                     LTFAALTGRKVITSLFGSRTAEEVLSSSVEHIRVAQEGDMLVVAPATADVLAKFAHGL
                     ADDFLSTLYLAFEGRVVLAPAMNTNMWRHAATVRNLEVLRERGHTIIEPEEGVLACGT
                     IGPGRLAEVEKICDTALTVPLRDLEGETILITAGPTQEPLDPVRYISNRSSGKMGFAL
                     AAAAAVRGARVILISGPVHLDAPKGVELVRVRTAVEMHDAVMEHLAESTIIVKSAAVA
                     DYRAAKVPEQKLKKTATRLSLELDPTPDILAELGQKKGDRLLIGFAAETEHLVEEARR
                     KLKSKNCDMIAANLVGQDNTGFESDENEVVLALSTGEAINIPRGSKRAVAERIFDEAL
                     RLRLALHAVR"
     gene            5286..6032
                     /locus_tag="VFQ79_01580"
     CDS             5286..6032
                     /locus_tag="VFQ79_01580"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0138364.1"
                     /translation="MNRELLKQYLEFYRDLGIETLYRRDAPPGPEAAAPVAEGAAPDA
                     APEQFLILPSIAPGDDSLEKIREDIGDCRRCRLHEGRNRIVFGTGDAQAKLVFVGEGP
                     GADEDEQGIPFVGRAGQLLTQMIENTAAREGIALKRPDVYICNIVKCRPPGNRTPEPD
                     EMEICGEFVFRQLSVIHPKAICALGGTAAKGLLGRKEGVTKLRGKWYKWRDIPVMVTY
                     HPSYLLRPYNQSAKREAWEDLKKALHYVYD"
     gene            6019..6684
                     /gene="tmk"
                     /locus_tag="VFQ79_01585"
     CDS             6019..6684
                     /gene="tmk"
                     /locus_tag="VFQ79_01585"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU0138365.1"
                     /translation="MSTTETSRGLFITFEGLDGSGKTTQLTRLAARLRSRGYEVVETI
                     EPGGTRTGSMIRNILLDPAHQELSATAELLLYFAARAQNVDEMIVPALKRDAIVLSDR
                     FTDSTLVYQGYGRGLGVEVVEALDRIACHGLKPDLTLYLDIDLATARSRAEARGIDRM
                     DDQPADFYRRVREGYLELSQREPRFRVIDGRGEIEAIDSEVWNQVRATLESSAPAGTP
                     GRL"
     gene            6804..8816
                     /locus_tag="VFQ79_01590"
     CDS             6804..8816
                     /locus_tag="VFQ79_01590"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEU0138366.1"
                     /translation="MSLSRFLKCCVFLVTSLPVLAQSQASSVRILLPERTRLLQNQLV
                     DIVLEVRNAPGISGLSVTAGGADVTSRFSGPVQADLDCDASSDWVLRANLMAFNTPGS
                     IKLDVSLSAGGVTVRDSRTILVQEFNLRPDQRRNIILFIGDAMGTAYRDAARLVSRAI
                     VDENGRNSFREGFFDDLLEMDKMPVSGMVMTYGTDSIVPDSANTGTAWATGNKSFLNA
                     VNSFTDGTDCKWRFNGQSNAANLVYITDNPRVESLWQYLKRRFGYRAGIVTTAAVTDA
                     TPAVQGAYVGFRQARLEIARHYVENPMLGGRPAFDVILGGGADPFTSAGRTDRRNLIA
                     EFQSRGYQYVTNASQLRSANGNTVGLFKGSAAPAPASNGIATASDVNMDVAYDKLGIP
                     RPASEPAPNLGNFTDQPMLDAMTEAALRMLSDSTAPAPFVLMVEGASIDKQSHPNQAA
                     GTIWDTIELDKAIGVTRSWAARRPSKDTLIVVTADHDQSMHIIGVSNTPDAEYFDRNR
                     NQKITFSTGAGNQDFTVWGDSYTNARAGLPFINSSTGASNNGGVTAMPGTFKRSDSTD
                     DPASSTYSTYFGSPAYTLDPKTGYPNNSGSKLRRLAVGFRTGDHTGSCVPVTAEGPGA
                     FLFTGYMDQTDIFFKMAATLSIDTKEGDKFVDTILTGNAFPKTFGK"
     gene            8820..9365
                     /locus_tag="VFQ79_01595"
     CDS             8820..9365
                     /locus_tag="VFQ79_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138367.1"
                     /translation="MSLSHLIYGALLSAALLFSQEHETPAAGPVLFTLEETREQVARA
                     MGSPDLVANFGTDLLSWQFRLGGVDHHSGGCTAGEYSHQLVFRKSTGKLVSVTVNFEH
                     DQKVDALFPEDETAIHHYPDAARPQLSVALRRLPGGRALIATGMPKPGRSAAQLLLIR
                     ESEIRHFLPWLYERLQPGETR"
     gene            9405..9668
                     /locus_tag="VFQ79_01600"
     CDS             9405..9668
                     /locus_tag="VFQ79_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0138368.1"
                     /translation="MHRISGFLFAGALLFLSCGSEIKSPKELTAGELEKLLASKDNIF
                     YLDVREPEEIQQLGSVRGYVNIPIGQLEARLKEIPKDKLIVTL"
     gene            9839..11347
                     /locus_tag="VFQ79_01605"
     CDS             9839..11347
                     /locus_tag="VFQ79_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0138369.1"
                     /translation="MRFLWVAMALVAAGQEPPTIRVTTRIVQVSVIVQDSHGRPMRGL
                     AREDFQLFDAGVEQEIRSFVVEDDTGRAAPLTPDVFSNRYGEQAGVTVILLDQLNSLL
                     EDEAHGRSELLRFLRHAEPHHRIALLSLSSELEILHELTSDAASIARALERFLPGRSV
                     TAAAIRPETLPEFQRIDSLMEAGKARARAGETTLAIEVLGRYLARIPGRKNLIWMSSV
                     FPGTLAARVRQDDVAIYPIDVRGVKAPEDPDVPPAPLMDGVMYGARALAAQTGGVAFY
                     GTNDLRLAIQQAHEDARGAYLLEYYPSHERWDGAYRALQVSVRRPGVHVRHRMGYFAS
                     AEQIERSPQNRREAILSAASSPADLVAIRFDVQINRGSEGVQTITVLIDPQTLSFTQL
                     GGRWRDELDLGIVAQKENGEGFPGRIQAYNIDVPERTFRELIAQKGVSLTSKLTLPEN
                     AYRLRVVVREVRTGATGSVTIPLREITGSRAAGGTEKEAPPAARFGGESTQR"
     gene            complement(11338..12042)
                     /locus_tag="VFQ79_01610"
     CDS             complement(11338..12042)
                     /locus_tag="VFQ79_01610"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138370.1"
                     /translation="MIRTLQDVIERAFAQLSQTTTAYLPPLLAGLIILFGAYVTAVLV
                     RLLLTRLIKGAGIDRFLQQSGVSYMFDRSGRVRTSRLIAGSAYWVILGIGFLTAVSAF
                     NTGLTNRMVEGVVLLFPKLVTAGLILLAGAWLAQYLGRSTLVWAVNEGLPSPRKIAMA
                     IRTVIMFVAVVVAADHLDFARSVFLAAFVILVGGAVLAASIALGLGAKGAVQRHFQDR
                     AEHAEETPERSLWNHL"
     gene            complement(12042..13379)
                     /locus_tag="VFQ79_01615"
     CDS             complement(12042..13379)
                     /locus_tag="VFQ79_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall biosynthesis glycosyltransferase"
                     /protein_id="HEU0138371.1"
                     /translation="MPNHLLSEEAQGALDRIERADIVVGIPSYNNVRTIGHVVRAAQA
                     GLAKYFPQFSSLIVNSDGGSKDGTREAVMSVQVEDTHLMLLSTPMPPVHRLSFPYHGI
                     PGKGSAFRMIFQMAELLRAKACVVVDSDLRSITPEWIDLLVRPVLYAGYDFVAPYYHR
                     HKYDGTITNSIVYPLTRALYGLRVRQPIGGDFGISGRLVSRYLKRNDWETDVARYGID
                     IWMTTIAIAEGFRVCQSFLGAKLHDAKDPGADLSAMLHQVVGSVFSLMQEYESAWENR
                     SGSDPVDLFGFRFDVGLDPIEVNLDRMFNAFLRGCRDLGELWTMALHPDTYEDISRLA
                     RSLERDRGAFHLSDELWARVIVDFACAYKQRPLERSHLLPSLTPLYLARVASFVLETQ
                     NLFSAQVEERIENLCLAFEGLKPYLRARWKGETAPPQARREDSALKKEKLEVQ"
     gene            complement(13454..14701)
                     /locus_tag="VFQ79_01620"
     CDS             complement(13454..14701)
                     /locus_tag="VFQ79_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138372.1"
                     /translation="MADFHQTGVVTTLHNLDNNGLNRLENELGAFSDRVPIGLVLPAL
                     YSEFEKPAMGRIISELSKVRYLRQIVVALGRADAAQFEHTKSLFRDFHCPVTILWMES
                     PRIQTLLSMLGENGLSAGDDGKGRSCWMAYGYLLAAGHCDVIALHDCDIVNYDRKMLA
                     RLCYPVAHPNLGFEFCKGFYARVTNRMHGRVTRLFVTPLVRSLDGMASGAPFLRFLDS
                     FRYPLAGEFAMKTNLARVNRIPGDWGLEVGVLAEVFRNCATTRVCQVDVTDNYEHKHQ
                     ELSPEDASKGLRRMTVDIAKSLFRTIAGEGVVLTEDHFRSLQVRYVRMAEDTIQRYYA
                     DAMVNGLEFDRHSEEMAVAAFAKSLREAAAEFVEDPLGVPLIPNWNRVVSAIPEFFNL
                     LLDGVEQDSQLGAATESARFAGR"
     gene            complement(14800..17769)
                     /locus_tag="VFQ79_01625"
     CDS             complement(14800..17769)
                     /locus_tag="VFQ79_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HEU0138373.1"
                     /translation="MQRLVEEVEFRDRARAVSIAGRFARTLTPTIQELIRYLLSGSAD
                     PDSALHWLDCLYSQNRMSFEKIIRSPVTLQYFVAVASHSRFLSEAVVQQPEWLEELPQ
                     SGDLHRVLSSEELEERLDQFLSRGQESESTERLPRAPDGTPSLLELARFRRKNILRIL
                     LRDVMAFTGLAEITEDLSNLADAVLDLCYLRIRAGLVERYGTPRYKDPSGRVRECGFS
                     VIALGKLGGRELNYSSDIDLMFLYSGPGETSGPNPISNKEFFKKVANELTTLLSTYTA
                     EGMCYRVDLRLRPDGRFGEVCMSVDGAKSYYQNRARDWELQMLIKARVAAGERAPGRE
                     LLSFVEPIIYSTTLDFSAVESVSATRERIAEKLQTRRSQAAFDIKLAPGGIRDIEFLV
                     QCLQRLHGGREAWVRHGGTLQALFRLHDKGLLSGPEYGRLASAYQFLRTLEHRLQFHE
                     DRQTHALPTDMQELEILSRRMPASEIGRAPSAENLLRTLNRHLEEVQIIYERVIHAQN
                     PTYYTAAATFPAAGVREEASVTMSVPEKTDVPTTASNLIRFLDQKAPGLAAVLARNPL
                     KRGMNTLELFLERVLPNSEWMRWLDSDAVLTGYLLDLFDNSPYFAEQMVRTPDLLSDL
                     MSMRERPGRDPRYTELAAVITEASALRRFYRREMFRIQAESVCTGAPIFSTLVKTSEL
                     TDVTISTAYRMAVEHVLAAKGPQSADYAAEDQLMVIALGRLGMREFDLASDADLVFVL
                     PDADAGELVFWTRVAERLIDVLTAYTGEGVMFTVDTRLRPNGREGALVQLERTYKEYF
                     AERAEAWEGITYMKSLAVAGDLERGTQFLNSLQEVDWRRYGQSGRSKKLLRQMRMRLE
                     KEQGAANPLKAGPGGYYDIDFSLMFLRLKGAGIFFKVLNTPQRIEVIEKMGHLERNDA
                     IFLNDAATLYRAVDHGLRVFTGHAEGDLPHAASQLEVLTELVKRWTPEHLHDQPLRTE
                     VEQIRSRTRDFFDRLFA"
     gene            complement(17770..18264)
                     /locus_tag="VFQ79_01630"
     CDS             complement(17770..18264)
                     /locus_tag="VFQ79_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU0138374.1"
                     /translation="MQGTVLQVNISPGGLPKYPIREAIVTPFGIEGDSCAHPEVHGGP
                     EKALLIVCSEVIEELKERGYPLFAGALGENLTTAGLDHRQLRTGQQFRVGSAFIELTR
                     VRRPCSSLNAIGSGLQTELFDAAVKAGDTRSPRWGMSGFYARVLRPGTVRPNDIIQLV
                     GQVV"
     gene            18360..20738
                     /locus_tag="VFQ79_01635"
     CDS             18360..20738
                     /locus_tag="VFQ79_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138375.1"
                     /translation="MQDFEKLGAFYLGRHHNLPQGKSGDEPVLYDSKDLLTHAVCVGM
                     TGSGKTGLGIGLLEEAAIDGIPALIIDPKGDLTNLLLNFPELEPEQFLPWVDEDGATR
                     RGLSKEEYAREQAELWRNGLVKWGQTPERIRKLRDSAEFSIYTPGSDAGIPVSVIASF
                     AAPPENIRNDRDLLRDSVSATTTSLLALIGVEADPLKSREHILIATIFDRAWKNGEDL
                     DLGSLIQQIQNPPVNRIGVFDLESFYPAKDRFGLAMALNNVLAAPGFEVWLSGEPLDV
                     NRLLYTAQGKPRMAIFSIAHLSDSERMFFVSLLLGQTVSWMRTQPGTTSLRAILYMDE
                     IFGYFPPVANPPSKLPLLTLLKQARAFGLGVVLATQNPVDLDYKGLSNAGTWFIGRLQ
                     TERDKARVMEGLEGAAAAAGSGFNRGAMEQTLAGLSSRIFLMNNVHEDAPVIFETRWA
                     LSYLRGPLTRPEIKRLMSARGAPAAEPKEAPRFAAPPSREAGPRPALPPEIPQYFFPS
                     HGSVVYEPMLLGIAQVRLVDAKNKVDETRDFAYLTRINDDVLAVNWDDARLEEIDAAA
                     LEQTPVEESRFVTLPPAGARAKSYAGWSKDFVSWLSSNAVLNLWRSTSLGEVSRPGET
                     ERDFRLHVQQLSREQRDRAIEELRQKYAPKLATLEDRIRRARQAVEREQQQSQGQFLA
                     VAGSIASTLAGALFGRKTLSATNLRRAGTAARQVSRSRKESGDVQRALETVEALETQR
                     EQLDEQLQAECDRIRSEFDSSAGSIEELPVRPRKSNISVRLFGLAWVPQTAP"
     gene            complement(20802..23123)
                     /locus_tag="VFQ79_01640"
     CDS             complement(20802..23123)
                     /locus_tag="VFQ79_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138376.1"
                     /translation="MFQSPNQLGRRAFLVAALGSGAITSYGAPQGRQTYVVVKPGTHP
                     GIIFSPQELPALRRRAKGTGLAAEAYAKVRRTAYESYSADLSVKQLVGREGNRLAEQL
                     ESMALVYQIEGDRDLARKAIDLFQSVASGCDPREFFRTVDSDFFATEHWPKAFAFAWD
                     WLYPEISAADRKTLLGSLENWSAALYEHTEAWWWREASYNCGAIPVGANGLLLAAMQA
                     ESQHLDFRRWFSECFRKVKENYFPLTWRASGICNEGPGYAHYHKNPTQFADAVRRTGG
                     PDIIGESGAVNAMHYLRHQWMPQGRCGTVGDNTEYGRRVFQAIYLHGIRELGDQAGLW
                     TFEKYADRERTEAFLQFLYYPDGLEPVSPGKLDLPTSFYFEIDRNRAGYVFARNEWDN
                     EQASWFAFTTRYANANHTHYDMNSFVFTAFGEEFAAHTNIFPYQHPHHGADLEHNIVI
                     VDDGGMPAADRPNSAGDDGSLYGYMTGVGVGRFGDYMRGDAHRSYADRSIKTDTPAVR
                     ADRTMVFAKRGANPYIVIADEIQRDDSEHNYHWQWYTMAHELSGRGTFDEPYLIEGRN
                     ARCKIVFLEPQAPEHEFRIVKGEQRRHGFDLGLLRVTLRGVQARYIALAASWRKDAPE
                     PAVRRGPEVKGAAGAASFVVEGADFRDLIVWQPGASGQPLSCGDVETNGLMTVVRTAG
                     DGTVTGYILGDGASLRFADRTLVDAPRPLAVSADTRYLSAMGARRAHENLPPFAAEGA
                     VWLPSPGAEVWGDGTRITPHMRNGGMAVIAPGS"
     gene            23282..23569
                     /locus_tag="VFQ79_01645"
     CDS             23282..23569
                     /locus_tag="VFQ79_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138377.1"
                     /translation="MKDDSAEAEPARKSRISPAGLKRISEASKRRWANARKAQAAAAP
                     PARTKKKSAAPRRPVRKKGGLTPEGRARLAEATRQRNIARAAAKKTAAKAG"
     gene            23586..25235
                     /locus_tag="VFQ79_01650"
     CDS             23586..25235
                     /locus_tag="VFQ79_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU0138378.1"
                     /translation="MHLTFVDYLILALYFAFVLGIGWLLKKRVSTSEDFLTSGRSVPV
                     WITSLAFVAANLGAQELIGMSASGAKYGIMTAHFYWVGAIPAMIFVGVFMMPFYYGSR
                     ARSVPEYLKLRFDEKTRGLNAITFALMTVFSSGISMYALGLLFQLVLGWSFTASVLLS
                     AGIVLVYTFLGGLTSAIYNEVLQFLLIVLGFIPLAYLAVSKAGGWEGMAERLPRVMTH
                     SWKYAGDASQNPMGVEIFGLVAGLGFVLSFGYWCTDFLVVQRAMAAESMSAARRTPLI
                     AAFPKMIIPFIVIVPGIAAVALSSMDIGYSLPVKGDSYDYDQVLTTLMAQFYPSGMLG
                     VGLTALMASFMSGMAGNVTAFNTVWTYDIYQSYIRPNAPDRHYLWVGRITTIVGVALS
                     IATAYLAQLYNNIMDLLQLVFGFVNAPLFATFLLGMFWKRSTGHGAFTGLVAGTSAAA
                     LTHGLTMAEGKGAWLGTVHTFPSIMAQNFWIAIFAWTTCFVVTIAVSLPTTPKPVSEL
                     EGLVYGVTKIPRDHGAPWYERPGLLAIIVGAVCIALNFLFW"
     gene            25244..25426
                     /locus_tag="VFQ79_01655"
     CDS             25244..25426
                     /locus_tag="VFQ79_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138379.1"
                     /translation="MDLRKPSGLFFTLLGAILLAVSVTGPGRHAPLTDVNVNLYSGIG
                     MLVFGGFLLWMAHRHS"
     gene            25402..26565
                     /gene="galK"
                     /locus_tag="VFQ79_01660"
     CDS             25402..26565
                     /gene="galK"
                     /locus_tag="VFQ79_01660"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU0138380.1"
                     /translation="MDGTPAFLKSRFKETFPLSGEPKIFRAPGRVNLIGEHTDYNLGY
                     VLPIALDLASYAVAAPNTERKLRIHSDGAGETREWPVSSIIDLEPAHDWSDYVAGVAR
                     ELIMAGFDVHPANLLVHSTVPLGSGLSSSAAFEVSVALALLNGRDIPPLELVHLCQRA
                     ENRFVGMPCGVMDQYASVFGRAGAALQIDCRTRQHQPVPLPDGVAIIAVNSMVKHELG
                     KTAYRERVDECAAAVQIIQERHPLVKSLRDATMRHLETLGPAMPQLVYRRARHVITEN
                     DRVMEFVKACEAADLECMGRKFRESHEGLRDDYEVSCEELDFLVETALTMPAVFGARM
                     TGGGFGGCTVNLVAPQASAEFKSAIVRKYEERFGIAPAVYDCHPSAGAGEMKF"
     gene            26787..28511
                     /locus_tag="VFQ79_01665"
     CDS             26787..28511
                     /locus_tag="VFQ79_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0138381.1"
                     /translation="MRVPLLLLTVAVAAGAQVNPAQQANPTQNVTPGSNQVQSGQPMP
                     VYRVTVVSKSTKAVNYRHRGGATKIDFKGTALMPRASGEAKVESKQGYLEIEVEFDEL
                     HPATQYGSEYLTYVLWAITPEGRAQNLGEIILNRDRSKLNVTTELQAFGLIVTAEPYF
                     GVTQPSDVVVMENVLRSDTKGAFDVVDAKYELLKRGQYTMNVDPSAVQPIAPIGTDKN
                     TPLELYEARNAVRIAQWAGADKYAADTFNKAQQSLQRAEEYRERKAGKRPIAMMSREA
                     VQTAEDARLIAIQRQEEERLANERKAAAEREAAAKAKAAEEALQRAEADRRRAQAEQD
                     RLAAERAQQAALLAKQEAEAAAARAAQERAAADEARLKMEAEAERARLAAEEADRLRQ
                     RAEDEKTQLRDQIRNQLSMILETRDSARGLIVNMSDVLFDFNKYTLKPDAREKLAKMS
                     GIVLAHPGLKLAIEGHTDSVGSDEYNQKLSEQRAGAVRDYLVQQGVQDPTATGFGESR
                     PIASNDNAAGRQQNRRVENRRLGRADRHRPDSQHERHTAAVNGEARRRAIHPAAACCH
                     SALACSFR"
     gene            complement(28481..28759)
                     /locus_tag="VFQ79_01670"
     CDS             complement(28481..28759)
                     /locus_tag="VFQ79_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU0138382.1"
                     /translation="MAYIIAEPCIGTKDTACVDACPVDCIHPRRDEQNFASEEMRYID
                     PEQCIDCGACVPVCPVSAIFAESDLPAQWQEYAERNRAHYLKEQASAE"
     gene            complement(28777..29139)
                     /locus_tag="VFQ79_01675"
     CDS             complement(28777..29139)
                     /locus_tag="VFQ79_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0138383.1"
                     /translation="MIVSSSSEDRLALASALEGHPCPQYWATSLQESLDQIERTPPRV
                     VICDANLPDGSWRDLWDHLRFPPDPPVFLVMETNADERLWAEVLNVGGYDVLVKPFDV
                     FEVFQVVQQACHTSSAPH"
     gene            complement(29330..29983)
                     /locus_tag="VFQ79_01680"
     CDS             complement(29330..29983)
                     /locus_tag="VFQ79_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0138384.1"
                     /translation="MLEAELFALLASTADAAFSLTESGEILSWNKAAERLFGFSSEQV
                     LNRRCYEILDARGRLGAAICMRDCVVQRCALHAGPVPDFDMEARTLSGGTVWVNVSTL
                     FYRNVRNGRLIIVHLARDITSRKKQEELISEWVRLSRRVAALNSGVQGAEPAPTLSER
                     EIQILQLLASPAAPADVAGALGITPSTLRNHLHRINEKLGTKDRLSAVLNAMHRGLI"
     gene            complement(29970..30530)
                     /locus_tag="VFQ79_01685"
     CDS             complement(29970..30530)
                     /locus_tag="VFQ79_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU0138385.1"
                     /translation="MQQTNPIHELTAEHRVIEKAAALMEKMADQLTSGSGVDGSVLQN
                     LVQFLRVYADELHHGKEEAILFPALEAKGVPQAGCPLGGLVAEHRKGRTLVDELIRAS
                     GAYEENAASAREELIASLRALRELYNSHIWKEDNLLFPLAEKLLAGDDSERVKKQFLE
                     VDERIGTERIRKLTGFADQLDRHAGS"
     gene            30690..31436
                     /locus_tag="VFQ79_01690"
     CDS             30690..31436
                     /locus_tag="VFQ79_01690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0138386.1"
                     /translation="MQDIWTDLQYRIIRALAPPSSRLDESSFEGKSKLRELAGDSILD
                     EFRGKHIIEFGCGAGRQTVELALAGAGRVTGVDIQEDLLVSAREYAQAAGVADRCEFV
                     TSTEKLADVIFTMDAFEHFTDPEAVLRKMRASLKPGGYVYVVFGYTWLHPLGGHLLSI
                     FPWAHLLFSEKALMRWRATVRDDGATRICEVKGGLGKMTIGRFERLAASTGFRVENLE
                     LRPIRRAAWLHCRLTQEFLTSGIRCRLVPV"
     gene            complement(31461..32459)
                     /locus_tag="VFQ79_01695"
     CDS             complement(31461..32459)
                     /locus_tag="VFQ79_01695"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU0138387.1"
                     /translation="MRNLAIIGGGSWGTALALVLAPRFQRLRLWVYEQDLAARMAETR
                     INDVFLPDFRIPDGVEVSSDLSYALEEAEIVLGVMPSHHARRLYSQMAALVQTDVIFV
                     SATKGLESQSLLRISEVIRQSVEGQQEPRIAVLSGPTFAVEVARGEPTAVVISSAERE
                     AAETVQAAFSGPSFRLYTNTDPIGVEIAAALKNVIAIGAGICQGLGLGSNTRAALISR
                     GLAEITRLATALGGNPRTLAGLAGLGDLVLTCTGDLSRNRRVGIELARGQTLSDIVSS
                     MRMVAEGVQTTFAAVELARLHGVEMPIAQQMNAILRDGRPPREAIRELMERSLKGE"
     gene            complement(32456..33061)
                     /gene="plsY"
                     /locus_tag="VFQ79_01700"
     CDS             complement(32456..33061)
                     /gene="plsY"
                     /locus_tag="VFQ79_01700"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU0138388.1"
                     /translation="MTPGLVLLAAYIVGAIPFGYLLVRWKTGRDVRTIGSGNIGATNV
                     VRTTGRALGIVTLLLDIAKGFFAVWLTARLTNGNLLWMSMAALAVMAGHAFPVFLKFR
                     GGKAVASFIGAFLYLTPLPLAAVLVVFVVSVAVTRYISVGSVLAAGSFPLAVWIILHP
                     PAPLLAAALFAGAFIVWRHKSNIERLRAGKEHTFSFGGRRS"
     gene            complement(33058..33603)
                     /gene="thpR"
                     /locus_tag="VFQ79_01705"
     CDS             complement(33058..33603)
                     /gene="thpR"
                     /locus_tag="VFQ79_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU0138389.1"
                     /translation="MRLFAAVDIPQEVVDRLDRLISKWRPLADLRWSRAGNLHITTKF
                     IGEWPQERLEEMIACLRRIPLPGPIPIAVRGLGWFPHERSPRVFWAGVEAPPSLKELA
                     AATERVCEDLGIAAEKRPFSPHLTLARIDPGSRLEKLREAIAALDTRDFGNFTVDRFF
                     LYESKLRPGGSVYTKLAEFAA"
     gene            33655..34230
                     /locus_tag="VFQ79_01710"
     CDS             33655..34230
                     /locus_tag="VFQ79_01710"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU0138390.1"
                     /translation="MKHSSVLDRSFYCRGTIDVAKSLLGKVLVHGTAAGVIVETEAYL
                     GGDDLASHSSRGVTNRTRVIFGPPGHAYVYFIYGMYECLNLVAEPEGVPGCVLIRALE
                     PVAGIAIMRERRPRAKKLEDLASGPGKLTLAMNITRRDNGRDVTCGPLTVRSFPQTKQ
                     PEIVTTSRIGIRECADWPLRFHIAGNRCVSR"
     gene            34258..35616
                     /locus_tag="VFQ79_01715"
     CDS             34258..35616
                     /locus_tag="VFQ79_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU0138391.1"
                     /translation="MLKSTIFREYDIRGIAETDLNDTAVEQLGKGIGTYFRRRGGTKV
                     NLGRDTRLSSPRLRDALLRGLLQSGCNVTDIGVVPTPVLYYSAYKWQPDGAVMITGSH
                     NPPEYNGFKVVSGGSTIFGDDIQEIYRIIEAGAFETGSGMEDAADAIGPYVDELASLV
                     SFDRRIRVVLDAGNGAAGPAMHRLLERLNCDPVELFFEMDGNFPNHHPDPTLPENLVA
                     LKRAVKEHKAELGIAFDGDGDRIGAVDENGEIIWGDMLLLIFAREILTRKPGATFIGE
                     VKCSQVFYDEVRKLGGNAIMYRTGHSLIKAKMKEEHAELAGEMSGHIFFQDRYFGYDD
                     ALYAACRLVEIVARSGEPLSAQLRGLPKLVATPEIRVDCPDEVKFDLVRRVAEVLRKS
                     HETVDVDGVRVLFPEGWGLVRASNTQPILVLRFEATTQEKLAQYRKEVEDVLEAAKQA
                     VV"
     gene            complement(35619..36122)
                     /locus_tag="VFQ79_01720"
     CDS             complement(35619..36122)
                     /locus_tag="VFQ79_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138392.1"
                     /translation="MQVLQAGEHKYLLLELDQEIIFSILKQAGFEFKLHETPKTIVLE
                     LTAAERKAPLLLFDASDPGNLGWFSRCQFYVDGSTGRVLQTPLALANMRDRSDRVLPH
                     SIRVQIAKELPAGFRLPGKQPVTEQMIYAVLYNFANALMQVGVGVCGGSVIKPLAGRT
                     ESIGVRN"
     gene            complement(36141..>37239)
                     /locus_tag="VFQ79_01725"
     CDS             complement(36141..>37239)
                     /locus_tag="VFQ79_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138393.1"
                     /translation="IFSLLLAFALPGILGAQSLSIEEYSPKSGLVVPQHRVPKARFPF
                     IDVHNHQRSLSPERLRQLLADMDSINMRIMVNLSGGYGDRLAQTVQAMKSASKDRFAV
                     FSNIDFTGIDEPDYGKRAAAQLEQDVRNGAQGLKIFKNLGLDLKDGKGERIRTDDPRF
                     DPIFEMCAKLNIPVLIHTGEPKGLFEPMDKYNERWLELKRYPRRGRPPDRYPTWEAIM
                     AEQHSLFARHPKTKFINAHLGWLGGDLAALGKLLDRLPNVYTEIAAVVEELGRQPRFA
                     RQWFVQYQDRVMFGKDTWRPEEYPTYFRILETADEYFDHDRPHHGLWKVYGLDLPDEV
                     LKKLYYKNALKVIPGLNAAGFPDQASSPASR"
BASE COUNT         7649 a        11102 c        11259 g         7229 t
ORIGIN      
        1 tatctcggcg tgccgcaaca ggagatcccc aaatcgtttt tattcgccgg cgaaagaacc
       61 gaaggcggcg cgccatacgc cgtactgcat cccttcgcct caacaccgga aaagacctgg
      121 ccggcgaatc gctttctgga agtggcgcga tgggtccagt ccgagtggaa cctggaaccg
      181 gtgttcatcg gagctcccgg cgaagatctg agcgcgttct cggcttatcg caccgcgacg
      241 gcctcgctat ccggaacaaa gacactcatc gccggcgctt ctctgttcct gggcaacgat
      301 agcgggcctg cacacatggc tgccgctttt tccattccgc tggttgtgct ctttgggcct
      361 tccgatgcga cgatatgggg tccctggagg gcggcctccg agacgctggt ccggcagccg
      421 atggaagaca tcacttccgg ggaagtggtt caggcgttgc agaggttgcg ggtgagcgta
      481 tgaagcaatt gctgcgcatc ctccggtacg tgaagcccta cattccggcg cttttggcag
      541 cggtgctgct gatggccgct gtgggtgcta tgcaggcgct gacggcgttg ctgatcggcc
      601 cgattttcga ccgggttctc gatccttcca cggttgacgc cccggtgctg ctgttcaccg
      661 ttccgctttt gcacaagcag atctatcttc accaactcgc tccgtcgtcg ttccacaacg
      721 tctggagtac ggtggctttc ggaattctcg ccacgtttct gatgaagggc ctgtgcgatt
      781 acgcgggcaa ctatctgatc aactacgtcg gattctcagc ggtaaccgac ttgcggcagc
      841 gcgtgttcga cagcgttctt cgccaggatg cgcagttttt cgaactgcat tccaccgggc
      901 gtctgatgtc gtctatcatg aacgacatcg agaagatcca ggtggcgacg tcgcatattc
      961 tggcggactg gctgcggcag agtttttcgg ctgtctttct caccatcgtt gtgcttcagc
     1021 gcgactggcg cctggcagtg gccagtctca cggttctgcc attcgtgctg gttcccacgc
     1081 tccgtctcgg acgcaggttg aggcgcacta cccggcgcgc gcaagacgat gcggccgaac
     1141 tcaaccagat tctgcaggag acgcttagcg gccaccaggt agtgaagtcg ttcggggccg
     1201 aagccgagga gtccagccgg ttccgcgccg ccgcaaagaa gttccgttcc agcaatctgc
     1261 gttacgtggc gcagcaggct atcgcctctc cgctcatcga attttttggc gccgtcacca
     1321 tcgttgggct gctcacctat gcccggacgc agatcaaaaa cggcgatatg acgacggggg
     1381 aattcaccag cttcgtcatc gcgctcttca tgctgtacga gcctgtaaaa cgcctgacgg
     1441 gcattcacaa catcttccag caggccctcg gcgcctcgca gaaggttttc gggtatctcg
     1501 accgtcccgt cgccgtgcac gagcgaacgg acgcccgggt tttgaagcgc ttcaccgaaa
     1561 gcgtggtgtt tgaggacatc agcttccggt acccgaattc gccgaatgga tttcttttga
     1621 aaaaggtcca tctcgatgta aaggcgggtg aggtggtggc actggtcgga cctagcggcg
     1681 cgggaaagac gacccttgcc aatcttcttc cgcgcttcta tgatgtcacc tccgggaccg
     1741 tccgcatcga tggtatcgac gtgcgcgacc ttacgctcaa atcgctgcgg gagcagatcg
     1801 gcattgtcgc gcaggacacg tttttgttca acgatacggt ggccaacaac attcgctatg
     1861 gcagacccgg agcaagccgg gcagaggtgg aagaagcggc ccgcaatgcc cttgccgaag
     1921 agttcattct gcggctaccg gaaggctacg aatcctggat tggcgaacgc gggttgaaac
     1981 tcagcggagg ccagcgccag cgaattgcca tcgcgcgcgc gctgctcaag aacgccccga
     2041 ttctgattct ggatgaagcg acttcccacc tcgatacgga atcggaaatt ctcgtgcaaa
     2101 aggctttgtc gaacctgatg aaagatcgca cggtcatcgt cattgcgcac cgtctttcta
     2161 caatccggcg ggctgacaag attgtggttc tcgatggagg ccagatctcc gagaccggct
     2221 cgcacgacga gctggtgagc ggcggcggca tctaccagcg cctgcatgag cttcagttcc
     2281 tggaagccga tccgatggtg aacccatgat ccgcagcatg acgggatttg cccgggcgag
     2341 aaaaaccacg gaagacggcg agatcgtgct cagcgtaaag agcctgaatc accgcggcct
     2401 cgatttgcat tttcatttgc cggtggtgtt cgacgcgtac gaaaatgcga tgcgcgaggc
     2461 gctgaaacac aaagtggcgc gcgggcatgt ggacattcgc gttgtgtggc accccgtacg
     2521 ctccactgga tccgccggac taaatcgccc ccttctggac acttatatag ctggctttcg
     2581 cgaagcagcc cggcagtatg gaatcgccgg agaacccgat ctgaacgccg cgctgcgcgt
     2641 gcccgggatg ttcagcgata cggacgatgc ggaagtgagt cccggagtcc aggcggtgct
     2701 gatggcggca ctggcggagg cgctggagga gttgaatgct ttccgggcgc gggaaggcgc
     2761 gcagcttgcc tcgctcatgc tggaacgcaa ccaggcgatc gcatcggcgg ccgaacggct
     2821 ggaaacgctc cgattgggcg ccgttcaggc ttttgagaac cggcttacgg aacgtcttgc
     2881 cgaactgctg cgcggggttg ccgttgagcc gcagcgcctg gcgcaggaag cggccattct
     2941 ggccgaccgc agcgatatcg gcgaagagat cgcgcgtctt aagatccact cgggacagtt
     3001 gcagcaactg ctggaggggg gcaccgaaac gggaaaaaaa atcgacttcc tgctgcaaga
     3061 aatgaaccgg gaaaccaaca cgattctttc gaagagcacc ggtctggggg aaatgggcat
     3121 ggagatcaca aacctcgggc tggcggtcaa gtccgatatc gagaagattc gcgaacaggc
     3181 cctcaatctg gaatgacaac cgtttttatc atttccgcgc cgtccggctc cgggaaatcg
     3241 actctggtgt cgcgcctgct tcagcgcgat ccccagcttg ttttttccgt gtccttcacg
     3301 acccgcccgc cgaggggcgt cgaattggag ggcaaggact accattacat ctcgcgcgag
     3361 gagttcgaga agcggctgct tcatagcgaa ttcctggagc atgccgaggt gttcggcaac
     3421 tactacggca cgcaccggag catcctggac aaagcccggt cccagggaaa ggacctggta
     3481 ctcgacatcg acgtacaggg tgcgagacaa ttaaaagaca ggcttccgga tgccgtgagt
     3541 atattcatcc tcgcgccctc ccggcaggaa ttggaggagc gtctccgcag ccgatccgag
     3601 gattcccaag aggttattga gcggaggctg agggatgcag ccgaggagat caggaagtac
     3661 aggcaatacg attacattgt cattaaccgc caggtggaaa catccgtcaa tacgctggct
     3721 gccatcatac gcgccgagcg cgcgcgccgg tcgcggatgg aagacgagat ccgcccgatt
     3781 ctggaaacct ttgaaacata caaatgaggc cttagcaaca tgcccgagaa gttgacgaga
     3841 aatcaatcca ttcactctat tccggacgat acggaacaga gcgcttaccg gtttatcatt
     3901 ctttccgcga aaagagcgcg ccaattgcag aacggggcgc ggtcgttcct gcccactaca
     3961 tcgagaaagc cgaccattac ggccatggaa gaagtgcgcc gggggttggt gaagtattcc
     4021 gataccggtc gcgacgccga actgaatccc gtggccgagg agtcctgagc cggttctccc
     4081 atgaacgtca ttctgggagt cagtggaggc atagccgctt ataaggcggc ggagctcgca
     4141 cgcgcgttta tggagcgcgg gcactccgtt caggtagtga tgacggcagc agcgggagag
     4201 ttcatccagc cgcttacctt tgccgcgctc accggccgga aagtgattac cagcctgttc
     4261 ggttcccgca cggccgaaga agtgctgtcg agttcggtgg agcatatacg agtggcccag
     4321 gagggcgaca tgctcgtcgt agctcccgcc accgccgacg tgctggcaaa gttcgcgcac
     4381 ggattagccg acgattttct ttccacgtta tacctggcct tcgaaggccg tgtggtgctg
     4441 gcgcccgcaa tgaatacgaa catgtggcgg cacgccgcga cggtacgaaa tctcgaagtg
     4501 ctccgcgagc gcgggcacac gattatcgaa ccggaagagg gcgtgctggc ttgcggaacc
     4561 attgggccgg gccgtctcgc tgaagtggag aaaatttgcg acaccgctct gacggtgcct
     4621 ctccgcgatc tggaaggaga gaccattctg attacggcgg gtccgacaca ggagccgctc
     4681 gaccccgttc gctatatctc caaccgttcg agcggcaaga tgggctttgc tctcgcagcg
     4741 gccgccgcgg tgagaggtgc gcgggtcatt ttgatctccg gaccggtgca tctcgacgcc
     4801 ccgaaaggcg ttgaactcgt tcgagtgagg acggcggtgg agatgcatga tgcggtgatg
     4861 gagcatctcg ccgaatccac catcatcgtg aaatcggcgg ctgtcgccga ttatcgcgcg
     4921 gcgaaagttc ccgaacagaa gcttaagaaa acggcgacgc gcctgtccct ggaactggac
     4981 ccgaccccgg atatccttgc cgagctgggc cagaagaagg gcgaccgttt actgatcgga
     5041 ttcgccgccg aaacggagca cctggtggaa gaggcacgcc gcaaattgaa atcgaagaac
     5101 tgcgacatga ttgccgcgaa cctggtgggg caggacaaca ccggcttcga atcggacgag
     5161 aacgaagtgg tgctggcgct ttcgaccgga gaagcgatca acatccccag aggatcaaaa
     5221 cgagccgtgg cggagcgcat ctttgatgaa gcgctgcgac tgcgcctggc gctgcatgcc
     5281 gttcgatgaa tcgcgaactg ttgaagcagt atctggagtt ttaccgcgac ctgggtatcg
     5341 agacgctgta ccggcgcgac gccccgcccg gtcctgaggc cgccgcgcct gttgcggaag
     5401 gagccgctcc ggacgccgct cccgaacagt tcctcattct gccgagcatt gcgccgggag
     5461 atgacagtct cgaaaaaatc cgcgaggaca tcggcgattg ccggcgctgc cgcctgcacg
     5521 aaggcaggaa tcgtatcgtg tttggaacgg gcgacgcgca ggccaaactc gtctttgtcg
     5581 gcgaaggccc tggagcggac gaagacgaac agggcatacc cttcgtcggc cgcgcaggtc
     5641 aactgctgac gcagatgatc gagaataccg ccgcccgcga aggcattgca ctcaaacgtc
     5701 cggacgtcta catctgcaac atcgtgaagt gccgcccgcc aggcaaccgc acgccggagc
     5761 cggacgagat ggagatttgc ggggagtttg tgttccgcca gctttcggtg atccatccga
     5821 aggcgatctg cgcgctcggc ggtacggcgg cgaaaggcct gctgggccgg aaggaagggg
     5881 ttaccaaact gcgcggcaag tggtacaagt ggcgcgacat ccccgtcatg gtcacttacc
     5941 atccgtcgta tttactgcgg ccctacaacc agtccgcgaa gcgggaggcc tgggaagatc
     6001 tgaaaaaggc gcttcactat gtctacgact gagacatccc gcggactgtt catcactttt
     6061 gaagggctcg acggctcagg caaaacaacg cagctcacgc ggctcgccgc gcggctgcgt
     6121 tcccggggct atgaagtcgt ggagacgatc gagccaggcg ggacgagaac gggatcgatg
     6181 atccgcaaca tcctgctgga ccccgcgcac caggaattga gcgcaaccgc ggaactgctg
     6241 ctttactttg cggcccgcgc acagaacgtc gacgagatga tcgttccagc gctgaagcgg
     6301 gacgccatcg tcctttcgga ccggttcacc gattcgacac tggtgtacca gggctacggc
     6361 cggggccttg gcgtggaagt ggtggaagct ttggaccgca ttgcctgcca cggcttgaag
     6421 ccggacctga ctctatacct cgacatagac ctcgcgactg cccgttcgcg cgcggaagcg
     6481 cgcgggatcg accgcatgga cgatcagccg gccgattttt accgccgcgt gcgcgaaggc
     6541 tatctcgaac tatctcaaag agagccgcgc ttccgggtga tcgacgggcg gggtgaaatc
     6601 gaagcgattg actccgaggt ctggaatcag gtccgggcta cgctcgaatc aagcgccccg
     6661 gccggaacgc caggccgcct ataaccggcg atcggcagcc ggttccgcac ttactcaaca
     6721 tttattcagc cgtgattcag ttgtaacagt cggcttatac gattgaattt ctgtttttca
     6781 aacttacatc cgagagagtg tcgatgtcac ttagccgatt cctgaagtgt tgtgtcttcc
     6841 tggtcacctc gctgcccgtc ctggcgcaga gccaagcttc gagcgtgcga atccttcttc
     6901 ccgagcgaac ccggctgcta cagaaccagc tcgttgatat cgtactggag gttcgtaatg
     6961 cccccggtat tagcgggttg agcgtaaccg ccggtggtgc cgacgtgacc tcgagattca
     7021 gcggtccggt gcaggccgac ctcgactgcg atgcctcgag cgactgggtg ctgcgcgcca
     7081 acctgatggc cttcaacacg cccggcagca tcaagttgga cgtttcgttg tcggccggcg
     7141 gcgtaaccgt gcgcgacagc cgcacgatcc tggtccagga atttaacttg cggccggacc
     7201 agcgccggaa catcattctg ttcatcggcg acgcaatggg cacggcgtac cgcgatgccg
     7261 cgcgcctggt gtcgcgcgcc attgtcgatg aaaacggccg gaactctttc cgcgagggct
     7321 tcttcgacga tctgctcgaa atggacaaga tgcctgtaag cggcatggtg atgacctatg
     7381 gaacagattc catcgttccc gattcggcca acaccggaac ggcctgggcc acggggaaca
     7441 aaagcttcct caacgccgtc aacagcttca ccgacggtac ggattgtaag tggcgcttca
     7501 acggccagtc gaacgcggcc aacctcgtct acatcaccga caacccacgc gtggaaagcc
     7561 tgtggcagta cctgaagcgg cgcttcggct accgcgcggg aatcgtcacg acggcggcgg
     7621 tgaccgacgc cactcccgcg gtgcagggcg cctacgtcgg attccgtcag gcacgtctgg
     7681 agatcgcgcg ccactacgta gagaacccaa tgcttggagg ccggccggcc ttcgatgtga
     7741 tccttggcgg cggggccgat ccgttcacct ccgcgggacg cacggaccgc cgcaacctga
     7801 tcgctgaatt ccagagcagg ggttatcagt atgtaacgaa cgcctcgcaa ctacgctccg
     7861 ccaatggcaa cacggtcggt ctgttcaagg gatccgccgc gcccgcgccc gcctccaacg
     7921 gcatcgcgac ggcctcggat gtaaacatgg acgtggccta cgacaagctg ggcatcccgc
     7981 gcccggcctc cgaacccgcg ccgaacctgg gcaacttcac cgaccagccc atgctcgacg
     8041 caatgacgga ggcggcgctc cggatgctat cggatagcac tgcgccggcg ccgttcgttc
     8101 tcatggtcga aggcgcttcg atcgacaagc agtcccaccc caaccaggcc gccggcacca
     8161 tctgggacac catcgagctc gacaaggcca tcggtgtgac ccgctcctgg gcggccagac
     8221 gtccgtcgaa ggacacgttg attgtggtga cggccgacca cgatcagtcc atgcacatta
     8281 tcggcgtcag caacacgccc gacgcggagt acttcgaccg gaaccggaac cagaagatca
     8341 ccttttcgac gggggcaggc aaccaggact tcacggtatg gggcgattcc tataccaacg
     8401 cgcgggccgg ccttccgttc atcaacagca gcaccggcgc ctcgaacaac ggcggtgtca
     8461 ccgccatgcc gggaacgttc aagcgctccg actccacgga cgatcccgca tccagcacct
     8521 actcgactta tttcggttcg ccggcctaca cgctggatcc caagaccggt tatccgaata
     8581 actccggctc caagctgcgg cggttggcgg tcggcttccg caccggtgac cataccggct
     8641 cctgcgtgcc cgtcaccgcc gaaggacccg gcgcctttct gttcaccggc tatatggacc
     8701 agacggatat cttcttcaag atggctgcca ccctctccat cgatacgaaa gagggagaca
     8761 agttcgtcga tacgatcctc acaggaaacg catttccaaa gactttcggg aagtagccta
     8821 tgagtctgag ccaccttatt tacggagcgc tgctttcagc ggcgctcctt ttttcccagg
     8881 aacacgagac accggcggcc gggccggtgt tattcacgct ggaggaaaca agggaacagg
     8941 ttgcgcgggc gatgggcagc ccggacctgg ttgccaactt cgggacggat ctgttgagct
     9001 ggcaattccg gttgggcggc gtcgatcacc atagcggcgg ctgcacggcc ggcgaatact
     9061 cacaccagct cgtctttcgc aaatctaccg gaaagctcgt gagtgtgacc gtcaactttg
     9121 agcacgacca aaaggtcgat gcgttgtttc cggaggacga aaccgctatc caccactatc
     9181 cggacgccgc tcgcccccag ctcagcgtag ctttacggag gctcccggga ggccgggctc
     9241 tcatcgccac gggcatgccg aagccgggcc gttccgccgc acagttgctc ctcattaggg
     9301 aatccgaaat ccgccatttc cttccgtggc tatacgagcg ccttcagccg ggtgagacac
     9361 gctgattaca acgtgaccgg agcgggctga taaactcagg ccgtatgcat agaatttcag
     9421 gatttctttt tgcaggtgca ctcctgtttc tatcctgtgg aagtgaaata aagtctccga
     9481 aggaattaac ggccggggaa ctcgaaaaac tccttgccag caaggacaac atcttctacc
     9541 tggatgttcg cgagcccgaa gagatccagc agctcggcag cgtcaggggt tacgtcaata
     9601 ttccgatcgg acaactcgag gcgcggctga aagaaattcc caaggacaaa ctcatcgtca
     9661 cgctttgaaa ccggggagcc agagccgcgc gtgccgcggc catactcgag cggaacggat
     9721 ttcagacact gggctcctgc gggttggagg agtaccggaa caggaacaag ccgctggtat
     9781 atccgaagga ggggggcgga ggatgattgc cggataagac aatcacggag tgttggcggt
     9841 gagattcctc tgggttgcaa tggcgcttgt tgccgccggg caggagcccc cgacgatccg
     9901 cgtgaccacg cgtatcgtcc aggtgagcgt aatcgtccag gattcgcacg ggcgccccat
     9961 gcgaggcttg gcgcgcgagg atttccagct cttcgacgcc ggagtagaac aggagatccg
    10021 ctcttttgtg gtggaggacg ataccgggcg ggcggcaccg ctgacaccgg acgtgttctc
    10081 gaatcggtac ggtgagcagg ccggcgttac cgtgatcctg ttggatcaac tcaacagcct
    10141 cctggaggat gaggcgcacg ggcgttccga gttgctgcgg tttctgcggc acgccgagcc
    10201 gcatcaccgc atcgcactct tgtcgttatc ctcagagctc gagatcctgc atgagttgac
    10261 aagcgacgct gcttcgatag cgcgggcgct cgagcgcttc ctccccggac gcagcgttac
    10321 cgcggcagcg attcgcccgg agactttgcc cgaattccag cgcatcgatt cgttaatgga
    10381 ggcgggcaaa gcgcgagcgc gcgccggaga gaccacgctt gcaatagagg tgctgggccg
    10441 gtatctcgcg cgtattcccg gccggaaaaa cctgatctgg atgtcgagtg tgtttccagg
    10501 cacgctggcg gcgcgcgttc gtcaggacga tgtcgcgatt tatcccatcg acgtgcgcgg
    10561 agtgaaagcg ccggaggacc ccgatgttcc gccagcaccc ttgatggatg gtgtcatgta
    10621 tggtgccaga gcgctggcgg cacagaccgg gggcgtcgcg ttttatggga cgaatgacct
    10681 tcggcttgca atccagcagg cgcatgagga tgccagggga gcataccttc tcgaatacta
    10741 tccatcgcac gagcgctggg acggagcgta tcgtgcgctg caggtgagcg tacgccggcc
    10801 gggcgtgcac gtccgtcacc gcatggggta ctttgcgtcg gcggagcaga tcgagcgctc
    10861 gccgcaaaac cggcgcgagg ccatactcag cgccgcctcg tcaccggctg atctggttgc
    10921 gatccgcttc gacgtacaga taaacagggg ctcggaagga gtgcaaacca tcaccgtcct
    10981 gatcgacccg caaaccttgt cgtttacaca gcttggggga cgctggcggg acgaactcga
    11041 cctcggcatt gttgcgcaaa aagagaacgg cgagggcttt cccgggcgga tacaggccta
    11101 caacatcgac gttcccgagc gcacctttcg cgagttgatc gcacagaagg gcgtctcgtt
    11161 gacaagcaaa cttacgctgc cggagaacgc ataccgctta cgtgtggtcg tgcgcgaagt
    11221 gcgtaccggc gcaaccggct ccgtcacgat accactgcgg gaaataacag gcagccgggc
    11281 cgcgggaggg acggagaagg aggccccacc cgcagcccgt tttggaggag agtctactca
    11341 gaggtgattc cagagcgagc gctccggggt ctcttcggcg tgctcggccc ggtcctggaa
    11401 atggcgttgt actgctcctt tggccccgag ccccagcgcg atgctcgcag cgaggacggc
    11461 gccgcctacc aggatgacaa aagcggccag aaagacgctg cgcgcgaaat caagatgatc
    11521 ggccgccacc accacggcaa cgaacatgat caccgtgcgg atggccatgg cgattttgcg
    11581 cggtgagggc aatccctcat tgaccgccca taccagcgtg ctgcggccga gatactgagc
    11641 gagccaggcg cctgccagga gaatcagtcc ggcggtcacc agcttgggaa acaacagaac
    11701 tacgccttcc accatccggt tcgtcaaccc cgtattgaag gcgctgacgg cggtgagaaa
    11761 accgataccg aggatcaccc aataggccga gccggcaatc aggcgcgacg tgcggacgcg
    11821 gccgctgcgg tcaaacatat acgacacgcc gctttgttgc aggaagcggt cgatgccggc
    11881 gcccttgatg agcctggtca gaagcaaccg taccaggacg gccgtcacat aagcaccgaa
    11941 cagaatgatg aggccggcca ggagcggcgg caggtatgcc gttgtcgtct gagagagctg
    12001 tgcgaaagct cgttcgatca cgtcctgtaa agtccggatc attactgcac ctccagcttt
    12061 tcctttttga gggcggagtc ttcgcgcctg gcctggggcg gcgctgtctc acccttccat
    12121 cgagcccgca ggtagggctt caggccttca aaagcaaggc agaggttctc gatcctctcc
    12181 tctacctgtg ccgaaaacag attctgcgtt tcaagaacga aagaagcgac ccgggcgagg
    12241 tatagcggtg tgagcgaggg aagcagatgc gaccgttcaa gcggccgctg cttgtaggcg
    12301 caggcgaaat ccacgataac gcgcgcccat agctcatcgc tgaggtggaa cgccccgcgg
    12361 tcgcgctcca ggctccgggc aagccgggag atgtcttcgt aggtatccgg atgaagagcc
    12421 atcgtccaca attcgcccaa atcccggcag ccgcgaagga aggcgttgaa catgcggtcg
    12481 agattcacct cgatcggatc gagccctaca tcgaaacgaa atccgaacag gtcgactgga
    12541 tcgctgccgg agcggttctc ccaggccgat tcgtattcct gcatgaggct gaacacgctg
    12601 cccaccacct ggtgcagcat ggcactcagg tccgcgccag ggtccttggc gtcgtgcaat
    12661 ttcgcgccga ggaagctctg gcacacccga aagccctcgg caatggcgat ggtggtcatc
    12721 cagatgtcga ttccataccg ggccacatcg gtttcccaat cgttccgctt caagtagcgg
    12781 gaaacgagac ggccggaaat gccgaaatcg ccgccgatgg gctggcgcac ccggagcccg
    12841 tagagagcgc gtgtgagagg gtaaacaatg ctgttggtaa tggtcccgtc gtacttgtgg
    12901 cgatggtagt agggcgcgac gaaatcgtag ccggcgtaaa gcaccggccg caccagcaaa
    12961 tcgatccatt ccggcgtaat ggagcgcaga tccgaatcca ctactacgca cgccttggca
    13021 cgcaacaatt cggccatctg gaagatcatc cggaaggcgc tgcctttgcc cggaatgcca
    13081 tggtagggaa acgaaaggcg gtgtacgggc ggcatcggtg tggaaagcag catcaggtgc
    13141 gtgtcttcca cctgcaccga catcaccgcc tcgcgagtgc cgtccttgga gccgccgtcg
    13201 gagttgacga tgagcgaact gaattgggga aaatatttag ccaatccggc ctgcgccgcc
    13261 cgcacaacat ggccgatggt ccgcacgtta ttgtagctgg gtatcccgac gactatgtcg
    13321 gcccgctcaa tccggtccag cgctccctgc gcctcttcag acagcaaatg gtttggcatc
    13381 gttccgtcaa aaaatccagc ctgctagcgg cattcgagtc ctgcatttcc gtgaatccgc
    13441 gagcgtagcc ggatcatcgt cccgcaaacc gtgcgctttc cgtcgcggca cccaactgtg
    13501 aatcctgctc cacgccatcg agcaaaagat taaagaattc cggtatggca gagacgacac
    13561 ggttccagtt ggggatcaga ggaacaccta atggatcctc gacaaactcc gcggcagcct
    13621 cacgcagact tttcgcaaaa gcggcgacag ccatttcttc ggaatgccgg tcgaattcca
    13681 ggccattgac catcgcatcc gcataataac gctgaatggt gtcttccgcc atacgtacat
    13741 atcttacctg gaggctgcga aagtgatctt ccgtgaggac cactccttcg cccgcgatgg
    13801 tccggaagag cgatttcgcg atgtcgacgg tcatccgccg caggcccttc gacgcatcct
    13861 cgggcgagag ttcctggtgt ttgtgctcgt agttgtcggt cacgtccacc tggcagacgc
    13921 gggtcgtggc gcaattccgg aatacttcgg cgagcacacc cacttcgagg ccccaatcac
    13981 cgggaatcct gttcacgcgc gcgaggttcg tcttcatggc gaattccccg gccagcggat
    14041 accggaagct gtccaggaac cggagaaacg gcgcacccga agccattccg tccagcgacc
    14101 gcaccagcgg tgtgacaaac aggcgggtga cacggccgtg catccggttt gtcacccggg
    14161 cgtagaaccc cttgcagaac tcaaagccga gattgggatg ggcaaccggg tagcacagac
    14221 gcgccagcat cttccggtca tagttgacga tgtcgcaatc gtgcagtgca atcacgtcac
    14281 agtgccccgc cgccagcagg tatccatacg ccatccagca ggatcgccct ttgccgtcat
    14341 ctcccgcgga aagaccgttc tctcccagca tggaaagcaa cgtctggatt cgggggctct
    14401 ccatccaaag aatggtcacc ggacagtgaa aatccctgaa aagcgattta gtatgttcga
    14461 attgcgccgc atcggcgcgg ccgagagcta caacaatctg gcgcaggtat cttaccttcg
    14521 acaactcgct aataatccgg cccatggccg gtttctcaaa ctcggagtac aatgccggca
    14581 ataccaaccc gatgggaacc cggtcggaaa aggcgcctaa ctcgttctcc agccggttaa
    14641 ggccattatt atcaagatta tgcagagtcg tcactacgcc ggtctgatgg aaatctgcca
    14701 taggagatac ctctaatcat cagcgtaggt tgaatcccgc tccggaccta tacagcccat
    14761 aggggaggag gaacgggaaa agggactaaa ccgggttgtt caggcgaaca gccggtcgaa
    14821 aaagtcgcgc gtacggctac ggatctgctc tacttccgtc cgcagcggct gatcatgcag
    14881 gtgttccggg gtccagcgtt tcaccagttc ggtaagcact tcgagctggg atgccgcatg
    14941 aggcaaatcg ccttccgcat ggccggtaaa gacacggagt ccgtgatcca cagcgcggta
    15001 aagagtggcg gcgtcgttca ggaagatggc atcatttcgc tcgagatgcc ccatcttttc
    15061 aataacttcg attctttgcg gggtattcaa cactttgaaa aaaatgccag cgcccttgag
    15121 ccgcaggaac atcagggaga aatcgatgtc gtagtagccc ccgggtcccg ctttcagtgg
    15181 attggcggcc ccctgctctt tttccagccg cattcgcatt tgccgcagaa gctttttcga
    15241 acgcccgctc tggccatagc ggcgccagtc tacttcctgt aagctattga gaaattgagt
    15301 cccgcgttcc aggtcgccgg cgacggccag ggatttcatg taggtgattc cttcccaagc
    15361 ctcggcgcgt tcggcgaaat attccttata ggtccgctcg agttgaacca gggcgccctc
    15421 acgcccattc ggcctcagcc gcgtatcgac ggtgaacatc acgccttctc ccgtataggc
    15481 cgtcaggaca tcgatgaggc gctccgccac gcgcgtccag aagaccagtt caccggcgtc
    15541 ggcatccggc agaacgaaga caaggtcggc atccgaggca agatcgaatt cccgcatccc
    15601 gagacgtccc agggcgatga ccatcaactg gtcttcggcg gcataatctg ccgattgcgg
    15661 ccctttcgcg gcgaggacgt gctccactgc catgcggtac gccgtcgaaa tcgtcacatc
    15721 cgtaagctcg gaagtcttga cgagggtcga gaagatgggg gcacccgtgc agacgctttc
    15781 ggcctggatg cggaacatct cgcgccggta gaaacgccgc aaggcggaag cctccgtgat
    15841 gacggcggcc aactccgtat accggggatc cctgccgggc cgctcgcgca tgctcatcag
    15901 gtccgacagg aggtcgggag tccggaccat ttgctccgcg aaataagggc tgttgtcgaa
    15961 caggtcgagc agatagccgg tcagaacagc atcgctgtcg agccagcgca tccactcgga
    16021 attcggaaga acgcgttcga ggaaaagctc caaggtgttc atgccgcgct tcagagggtt
    16081 gcgcgcgagc acggcggcca ggccgggagc cttctgatcg agaaaacgaa tgaggttgct
    16141 ggccgttgtg ggaacgtccg tcttttcggg aacactcatg gtgaccgagg cctcctcgcg
    16201 aacaccggcg gcgggaaaag tcgcggccgc cgtatagtac gtaggatttt gcgcgtgaat
    16261 tacgcgctca taaatgattt gaacctcttc gaggtgacgg ttcagagtcc tgagcaggtt
    16321 ctccgccgag ggggcgcgcc cgatttccga ggctggcatg cggcgactca gaatctcgag
    16381 ttcctgcatg tccgtcggta atgcgtgcgt ctgccggtcc tcatggaatt ggagcctgtg
    16441 ctccagggtg cgcagaaact ggtaagcgga agccagccgc ccgtattcgg ggccggacag
    16501 gaggccttta tcgtgcaagc ggaaaagcgc ctggagcgtt ccgccatgcc gcacccacgc
    16561 ttcccgcccg ccatgcagcc tttggaggca ctgcaccagg aactcgatat cgcgaatgcc
    16621 gccgggcgct aacttgatat cgaacgccgc ctggctacgc cgcgtctgga gcttttccgc
    16681 aattcgctct ctggtggccg aaacggattc cacggctgaa aaatcgagcg tcgtggaata
    16741 aatgatgggt tcgacaaagc tcagcagttc ccggccgggt gctctttccc ccgccgcaac
    16801 cctcgccttg atgagcattt gcagctccca gtcccgggcg cggttctggt aatacgattt
    16861 cgcgccgtcc accgacatgc agacttcacc gaaccgtcca tcgggccgca ggcggagatc
    16921 gacccggtag cacattccct ctgccgtgta ggttgacagc agcgtggtga gctcatttgc
    16981 taccttcttg aagaattcct tgttggaaat gggatttggg ccgctggtct cgccgggtcc
    17041 ggagtacagg aacatcagat cgatatcgga gctgtagttg agctcacggc cgccgagttt
    17101 ccccaatgca atcacgctga acccacattc ccggactctg ccggacggat ccttatagcg
    17161 gggagttccg tatctctcca ccaggccggc ccggatccgg agataacaga gatcgagaac
    17221 ggcgtcggca aggttggaga gatcttcggt aatttccgcg aggccggtga atgccatgac
    17281 atccctgagc agaatgcgaa ggatgttctt gcggcggaac cgggccaact ccaaaagaga
    17341 cggggtccca tccggcgccc gcggcaaacg ctcggtgctt tcgctctcct ggccgcgaga
    17401 caggaattga tcgagccgct cctccagttc ttcggatgac aacacgcgat gcaaatcgcc
    17461 actctgaggc aactcttcga gccactcagg ctgttgaacc accgcctcgc tgaggaaccg
    17521 gctgtgcgaa gcgacggcga cgaaatactg aagcgtcacg ggtgagcgga tgatcttctc
    17581 gaaagacatc cggttttggc tatacaggca gtccagccag tggagcgccg aatccggatc
    17641 ggcggaaccg ctgagcagat agcggatgag ttcctgaatg gtgggagtga gcgtgcgggc
    17701 gaaccggccg gctatggaga cggcccgggc gcggtcccgg aattctacct cttccaccaa
    17761 gcgctgcatt cagacaacct ggcccacaag ctggattata tcgttcggcc tgaccgtgcc
    17821 cggtctcaga acacgcgcgt agaatccgct cataccccag cgtggcgatc gcgtatcgcc
    17881 cgccttcacc gccgcatcga acagctcggt ttgcagcccc gagccaatcg cgttgagcga
    17941 cgagcagggg cggcggaccc gtgtcagctc gatgaaggca ctgcctacgc gaaattgctg
    18001 tcccgtacga agctgccggt ggtccagacc ggcggtcgta aggttctcgc caagagcgcc
    18061 cgcaaaaagc gggtacccgc gctccttcaa ctcttcgatg acttcggagc agacgatcag
    18121 cagggccttt tccggtccac cgtgaacttc cggatgtgcg cacgagtcgc cttcaattcc
    18181 gaagggcgtt acgatcgctt cccgaatcgg atacttggga agaccgcccg gagagatgtt
    18241 gacctggagg acggtacctt gcattcttaa cgggcccgtg gcccgcttct ctcattgaac
    18301 cacggcggca ttacagtctt atcattcacc ggctgaaggc gattgtagac tggagaagca
    18361 tgcaggattt cgagaaactc ggcgcgttct atctcgggcg gcaccacaat ctgccacaag
    18421 gcaaaagcgg tgatgaaccg gtgctttacg attccaagga tctgctgacg cacgccgtgt
    18481 gcgtcggcat gaccggcagc ggaaagacgg ggcttgggat cgggctgctc gaagaggcgg
    18541 ctatcgacgg cattcccgct ctcatcatcg atccgaaggg cgacctcacc aacctgctgc
    18601 tgaactttcc cgagttggag ccggaacagt ttctgccttg ggtggacgaa gatggcgcta
    18661 cgcgaagagg cctgtccaaa gaggaatacg ccagagaaca ggctgagctg tggagaaatg
    18721 ggctggtgaa atggggacag acgccggagc gcattcgcaa gctccgcgat tccgccgagt
    18781 tctcgatcta tactccgggg agcgacgcgg gcattcccgt ttcggtgatc gcatccttcg
    18841 cggcgccgcc cgaaaacatc cggaacgatc gcgatctgct gcgcgacagc gtcagtgcca
    18901 ccacgacatc tctactggcg ctcatcggcg ttgaagcaga cccgctcaaa agccgcgaac
    18961 atattctgat tgccacgatt tttgatcgcg cgtggaaaaa cggtgaggat ctggatctgg
    19021 ggtctctgat ccagcagatt cagaaccccc cggtgaatcg catcggcgtc ttcgatcttg
    19081 aatcgtttta cccggcgaaa gaccgtttcg gtctggccat ggccctcaac aacgtgctgg
    19141 ctgcgcctgg ttttgaagtg tggctctcgg gtgagcccct cgacgtgaac cgcctcctgt
    19201 acaccgcgca gggaaagcca cgaatggcga tcttctcgat cgcgcacctg agcgactccg
    19261 agcggatgtt ttttgtatcg ctgctgttgg gccagacggt gagctggatg cgaacgcagc
    19321 ccggcactac gagcttgcgg gccattctct acatggacga gatcttcgga tattttccgc
    19381 ccgtcgccaa tccgccctcg aagctcccgc tgctgacgtt gctgaaacag gctcgcgcct
    19441 tcggcctggg tgtggttctt gccacgcaga atccggtgga cctggactac aaaggactgt
    19501 cgaatgcggg aacctggttc atcggccggc tgcagaccga gcgcgacaag gcgcgtgtga
    19561 tggagggctt ggagggtgca gcggcggcgg ccggctccgg gttcaaccgc ggagcgatgg
    19621 agcagactct cgcgggcctc agcagcagaa tttttctgat gaacaacgta cacgaggacg
    19681 ccccggtgat attcgaaacg cgctgggcgc tctcgtattt gcgcggcccg ctgacccggc
    19741 cggagatcaa gcggctgatg agcgcacgcg gcgcgccggc agccgaacct aaggaagctc
    19801 cccgtttcgc ggcgccgcca agccgggagg ccggcccgcg gcccgctctt cctccggaga
    19861 taccgcagta ctttttccca tctcatggca gcgtagtgta tgaaccgatg ctcctcggta
    19921 tcgcgcaagt ccgccttgtc gatgcaaaga acaaggtcga cgaaacgcgc gacttcgcct
    19981 atttgacgcg aatcaacgac gacgtactgg cggtgaattg ggacgatgcc cggctggagg
    20041 aaatcgatgc cgccgcgctc gagcagacgc ctgtcgaaga aagccgtttc gtcacgttgc
    20101 cgcccgcggg cgcgcgggcc aaaagctacg cgggatggag caaggatttt gtaagctggc
    20161 tgtcttcgaa cgcggtgctg aacttatggc ggagcacatc gttgggcgag gtgtcccgcc
    20221 cgggagaaac agagcgggat ttccgcctgc acgtgcagca gttgtcgaga gaacagcgcg
    20281 atcgggcgat tgaggaattg cgtcagaagt acgctcccaa actcgcgacc ttggaggacc
    20341 gcatccgccg cgcacggcag gcggtagagc gcgagcagca gcagtcgcag gggcagttcc
    20401 tggcggtggc gggctccatc gcgtcgactc tggcgggcgc gctgtttggg cgcaagacac
    20461 tcagtgcgac gaatctacgg cgggccggca cggcggctcg tcaagtgagc cgcagccgga
    20521 aagaatccgg tgatgtccag cgggcactcg agacggtaga ggcccttgaa acacagcgcg
    20581 aacagttgga cgaacagctt caggcggagt gcgaccgcat ccggtctgaa ttcgactctt
    20641 ccgcgggctc catagaggaa ctgccggttc gaccgcgaaa atccaacatc tccgtgcgac
    20701 tgttcggact cgcctgggtt ccgcaaaccg ctccgtgacc gtcgcggctc agaaacaaaa
    20761 gcgttacgga gccgcgcgcg ccagcaagcg ggcgtgactt ttcatgaacc gggagcgata
    20821 acggccatgc cgccgttccg catgtgcggg gtgatgcgag tgccatcccc ccatacctcc
    20881 gcaccgggag agggaagcca aacggcgcct tcggcggcaa agggcggcaa attttcgtgc
    20941 gcccggcgag cacccatcgc ggatagatac ctggtatccg cactgacggc caggggacgg
    21001 ggtgcgtcga ccagcgtgcg atccgcgaac cgcaacgagg cgccgtctcc aagaatgtag
    21061 ccggtcaccg ttccatcacc ggccgtgcgt acgactgtca tgagaccatt cgtctcgacg
    21121 tcaccacacg atagcggttg gccggatgcg ccgggctgcc acacgatgag gtcgcggaag
    21181 tccgctcctt ccacaacgaa actggcggca cccgcggcgc ccttcacttc cggaccacgc
    21241 cgcacggccg gttccggtgc atcttttcgc caggaagcgg ccagcgcgat atagcgagcc
    21301 tgaacaccgc gaagtgtcac ccgtagcaaa cccagatcga agccgtgccg ccgctgttcg
    21361 cctttgacaa ttcgaaattc gtgctctggc gcctggggtt ccaaaaagac gatcttgcag
    21421 cgggcgttgc gtccttcgat caaatatggc tcatcgaacg tcccgcggcc ggagagttcg
    21481 tgcgccatgg tgtaccactg ccagtggtaa ttgtgctcgc tgtcatccct ctgtatctcg
    21541 tcggcgatga cgatatatgg attcgccccc cgcttggcga acaccatcgt gcggtctgcc
    21601 cgcacagcgg gagtgtccgt cttgatggag cggtcggcat aggagcggtg cgcatcgccg
    21661 cgcatgtaat cgccaaagcg gcccacgcca accccggtca tgtaaccgta gagcgagccg
    21721 tcgtctccgg ccgagtttgg gcggtcggcg gcgggcatgc ctccatcgtc cacaatcacg
    21781 atgttgtgct ccaggtctgc tccgtgatgc gggtgctggt acggaaagat gttcgtgtgc
    21841 gccgcgaact cttcgccgaa cgccgtgaac acgaagctgt tcatgtcgta gtgcgtgtgg
    21901 ttggcgttcg catagcgggt ggtaaaggcg aaccaggagg cctgctcgtt gtcccactcg
    21961 ttgcgcgcga agacgtatcc ggcgcgattc ctgtcaatct cgaaataaaa agacgtgggc
    22021 agatcgagtt tgcccggaga taccggttcg agaccgtccg ggtagtagag gaactggaga
    22081 aacgcttccg tgcgctcgcg gtcggcgtat ttttcgaaag tccataaacc ggcctggtcg
    22141 cccagctcgc gaataccgtg cagataaatg gcctggaaaa cgcgtcggcc gtattcggta
    22201 ttatcgccga cggttccgca gcggccttgc ggcatccact ggtggcgcag gtaatgcatg
    22261 gcattcacag cgccggattc gccaatgata tcggggccgc cggtgcggcg gaccgcatcg
    22321 gcgaactgcg tcgggttctt gtgatagtgc gcgtagccgg gcccttcatt acagatgccg
    22381 ctggcgcgcc aggtgagcgg aaagtagttc tccttcactt ttcgaaagca ctcggagaac
    22441 cagcggcgga aatcgagatg ttgcgattcg gcctgcatcg cggccagcag aaggccgttc
    22501 gcacccaccg gaatggcgcc gcagttgtag gaagcctcgc gccaccacca ggcttccgtg
    22561 tgctcgtaga gcgcggcaga ccagttttcc aggcttccta acagcgtctt gcgatctgcg
    22621 gcggagattt ccggatagag ccagtcccag gcaaacgcga acgctttagg ccaatgttcg
    22681 gtcgcaaaga agtcgctatc gacagtgcgg aaaaactccc ggggatcgca gcccgatgcg
    22741 acggattgaa acaggtcgat ggccttgcgc gctagatcgc ggtcgccctc tatctggtag
    22801 accagagcca tggattccaa ttgctcggcc agccggttgc cttcccgccc caccaactgc
    22861 ttcacgctca ggtccgccga ataagattca taagcagtgc ggcggacctt tgcgtaggct
    22921 tccgcggcaa ggccggtacc ttttgcgcgc cgccgcaacg ccggcagttc ctgtgggctg
    22981 aagataatgc ccggatgcgt gccgggcttc acgacgacgt aggtctggcg gccttgcggc
    23041 gcaccatagg aagtgattgc tccgcttccc aaagcggcaa caagaaacgc gcgccgcccg
    23101 agttgatttg gactctgaaa cattggtcta cgggacgagt gtagcaaccc ggcaaccaga
    23161 tgacactaac ctgtgtagta ctgtagatct catggacaga tcagccgcag agctggcaat
    23221 caaagcactc gaagcagaac gccagaaaat cgattcagcc attcaagagc tgagatcctt
    23281 ggtgaaggat gatagcgcgg aagctgaacc ggcccgcaag agccgcatca gcccggccgg
    23341 cctgaaaagg atctcagaag catcaaaacg ccgatgggcg aatgcacgga aggcgcaggc
    23401 cgccgccgcg ccgcccgcac ggacgaagaa gaagtcagcg gcgccacgaa ggccggtgcg
    23461 gaagaagggt ggacttacgc ccgaaggccg cgcgaggttg gcggaagcga cccggcaacg
    23521 gaacatcgcc agggcggcgg cgaagaagac tgccgcgaag gcgggctgaa tagcgcgagg
    23581 ggcctttgca tcttaccttt gtcgactacc tcatccttgc actgtacttc gcctttgttc
    23641 tcggcattgg ctggctcctc aaaaagcgcg tctcgaccag cgaagacttc ctcacctccg
    23701 gacgctcagt gccggtctgg atcaccagtc ttgccttcgt agccgccaat ctgggcgcgc
    23761 aggaattgat cggcatgagc gcttcgggcg cgaagtacgg catcatgacg gctcacttct
    23821 attgggtggg agccattcca gccatgatct tcgtgggcgt gttcatgatg cctttctatt
    23881 acggaagccg cgcgcgctcc gtaccggaat acctcaaact ccggtttgac gaaaaaaccc
    23941 gtgggttgaa cgccatcacg tttgccttga tgacggtctt ttcctccggc atctccatgt
    24001 acgccctggg cctgctgttt cagctcgtgc tggggtggag cttcaccgcc tcggtgcttc
    24061 tgtctgccgg gattgttctg gtttatacgt ttctcggggg actcaccagc gcgatctaca
    24121 acgaagttct gcaatttttg ctgatcgttc tcggcttcat tccgctcgct tatcttgccg
    24181 tttcaaaagc gggtggttgg gaaggaatgg cggaacgcct gccccgtgta atgactcact
    24241 cctggaagta tgccggcgat gcttcacaaa atccgatggg ggtcgagatc ttcggtctcg
    24301 tagcggggct cgggttcgtc ctgtcgttcg gatattggtg tacggacttc ctggtggtgc
    24361 aacgggccat ggcggccgaa tccatgtcgg cggcgcgccg tactccgctg atcgccgcat
    24421 ttcccaagat gatcattccg ttcatcgtga tcgtccccgg gatcgctgcc gtcgcgctgt
    24481 ccagcatgga cataggttat tccctcccgg tgaagggcga cagctacgat tacgaccagg
    24541 tgctgacgac gttgatggcc cagttttatc catccggtat gctgggcgtg ggcctcacgg
    24601 cactgatggc gtcgttcatg tcgggcatgg cggggaacgt tacggcattc aataccgtct
    24661 ggacgtacga tatctaccag agctacattc gcccgaacgc ccccgacagg cactatctgt
    24721 gggtgggacg gatcaccacc attgttggcg tggcgctctc gatcgcgacc gcatacctgg
    24781 cgcagcttta caacaacatc atggacctgc tgcaactcgt gttcgggttt gtgaatgcgc
    24841 cgttattcgc aacgtttctg ctcggcatgt tctggaagcg cagtacaggg catggggcat
    24901 ttaccggcct ggtggcgggc acatccgcgg cggcgctgac gcatggcctc acgatggcgg
    24961 agggcaaggg tgcctggctt ggcacagtac acaccttccc gtccatcatg gcgcagaatt
    25021 tctggatcgc catattcgcc tggacgacgt gtttcgttgt gaccattgcc gtgagcctgc
    25081 ccactacacc gaagccggtc tctgaactcg aaggactggt gtacggtgtc acgaagattc
    25141 cgcgcgacca cggtgctccc tggtatgagc ggcccgggct gctcgccatc atcgtgggcg
    25201 cggtttgtat cgctttgaat ttcctcttct ggtgagtgct gccatggatc tgcgtaaacc
    25261 gtccggactc tttttcacgc ttctcggggc tattctgctc gcggtgtctg tcaccggccc
    25321 cgggcggcac gcgccattga ccgatgtgaa tgtcaatttg tattccggca tcggaatgct
    25381 ggtattcgga ggttttctct tgtggatggc acaccggcat tcctgaagag ccgctttaag
    25441 gaaaccttcc cgctttccgg cgagccgaag atcttccggg cgcccggccg ggtcaacctg
    25501 attggagagc acaccgacta caatctcggc tacgttcttc caattgctct ggaccttgct
    25561 tcttatgccg ttgccgcccc caataccgaa cggaaattgc gcatccactc ggatggtgcc
    25621 ggcgagacgc gcgagtggcc tgtttcaagc atcatcgacc tcgaacccgc acacgattgg
    25681 agcgattacg tggccggtgt ggcgcgggaa ctcatcatgg ccggcttcga tgtccatccg
    25741 gcaaacctgc tcgttcacag tacggtgcct ctgggttccg gcctgagttc ttcggcggcc
    25801 ttcgaagtct ccgtcgccct ggctctcttg aacggccggg acattccgcc gctcgaactg
    25861 gtacacctct gccagcgagc cgagaatcgg tttgtaggca tgccctgcgg agtcatggat
    25921 cagtatgcat ccgtgttcgg ccgcgccggt gccgcgcttc agattgattg ccgcacccgg
    25981 cagcaccaac ccgttcccct acctgatggt gttgccatca tcgccgtgaa tagcatggtg
    26041 aagcatgagc tgggcaaaac cgcctaccgg gagcgggtag acgaatgtgc ggcggctgtc
    26101 cagatcatcc aggagcgtca cccgctggtg aaaagcctgc gcgacgcaac gatgcgccac
    26161 ctggagacct tgggtccggc catgccgcaa ctcgtgtacc gcagagcgcg gcatgtcatc
    26221 acggagaacg accgcgtcat ggagtttgtc aaggcttgtg aagcagcgga cctggagtgt
    26281 atgggccgga aattccgcga atctcacgaa ggccttcggg atgactacga ggttagctgc
    26341 gaagaactgg atttccttgt ggaaacggct ctgactatgc ctgccgtttt cggcgcccgc
    26401 atgacaggcg gaggcttcgg aggctgcact gtcaacctcg tggcgccaca agcctcggcg
    26461 gaattcaaat ccgctattgt ccggaagtac gaagagagat tcgggattgc tccagccgta
    26521 tatgattgcc atccgtcggc gggcgccggc gaaatgaaat tctgataata tgggtctttc
    26581 aggagagaga ttttacagcc ggaatcacta cattagcaaa gcacgatagt gttcctcccg
    26641 gcgcgaaaca tcgccgcttg ctgcccgccc atgagatttg ctgtgtaaaa gatactcatg
    26701 cgcgggcaga cttagcccac caaatcaatt atttaccaat ttggcttttc gcgtgctaaa
    26761 aattgacagg aggaggggtt tgtactatga gagtgccctt gttgttattg acggttgcgg
    26821 ttgcagccgg tgcgcaggtc aatccggctc aacaggcgaa cccgacccag aatgtaaccc
    26881 caggtagtaa ccaggttcaa tccgggcagc cgatgcccgt ttatcgcgtg accgtggttt
    26941 ccaaatccac caaggcggtc aactaccggc atcgtggcgg cgcgacgaaa atagatttca
    27001 aaggtacggc cttgatgcct cgcgccagcg gagaagcgaa ggtcgaaagc aagcagggtt
    27061 acctggaaat tgaggttgaa ttcgacgaat tgcaccctgc tactcagtac ggctccgaat
    27121 acctcactta tgtcttgtgg gccatcactc ccgagggccg ggcgcagaac ctcggcgaaa
    27181 tcattctgaa tcgcgaccgc agcaaattga atgtgacgac ggagctgcaa gccttcggat
    27241 tgatcgtaac cgcggaaccc tacttcggcg tgacgcagcc gagcgatgtc gtggtaatgg
    27301 agaacgtact gcggtcggat accaagggag cctttgacgt tgtggacgcg aaatacgagc
    27361 tgctgaaacg tggccagtac acgatgaacg tcgacccgtc ggcagttcag ccaatcgccc
    27421 ccataggcac agacaaaaat acgccgctgg aactgtacga ggccagaaat gccgtccgca
    27481 ttgcgcagtg ggccggcgcc gacaaatatg cggcagacac gtttaacaaa gcgcagcaat
    27541 ccttgcagcg ggccgaggag tatcgggagc gcaaagccgg caaacggccg attgcgatga
    27601 tgtcgcgcga agcggttcag acggccgaag acgcgcgtct gatcgcgatt cagcggcaag
    27661 aggaagagcg gctggccaac gaacgaaagg ccgctgcgga gcgggaagcg gccgccaaag
    27721 ccaaggccgc ggaggaagcg ctccaacgcg cggaggcaga ccggcgccgg gcccaagccg
    27781 agcaggaccg gctggcggct gaacgcgcac aacaggctgc gttactggcc aagcaagagg
    27841 ccgaagcagc agcggctcgt gccgcgcagg agcgcgcggc cgccgacgag gcacggctga
    27901 aaatggaagc cgaggccgag cgcgcccgcc tggccgccga agaagccgat cgtttgcgcc
    27961 agcgcgcgga agacgaaaag acacaactgc gcgaccagat ccgcaatcag ttgagtatga
    28021 ttctcgagac gcgcgatagc gcccggggct tgatcgtgaa tatgtcggac gtgctcttcg
    28081 acttcaataa atacacgctg aaaccggacg ctcgcgaaaa actggcaaaa atgtctggca
    28141 ttgttctggc gcatccggga ctgaagctcg ccattgaggg acacacggat agcgtaggaa
    28201 gcgacgagta taaccagaag ctgtcggagc aacgcgccgg tgcggtccgc gattacctgg
    28261 tgcaacaggg tgtgcaggat cccacagcca caggttttgg cgagagccgg ccgattgcca
    28321 gcaacgacaa cgcggccggg cgccagcaga atcgccgtgt agaaaatcgt cgtctcggga
    28381 gagccgatcg gcaccgaccc gacagccagc atgagcgtca caccgcagca gtaaacggtg
    28441 aagctcgccg ccgggctata cacccggcgg cagcttgctg tcattcggca ctggcctgct
    28501 ctttcagata gtgagcgcga tttcgctcgg cgtattcctg ccattgtgcc ggcagatccg
    28561 attcggcgaa aatagccgaa accgggcaca ccggcacgca ggcaccgcaa tcgatacatt
    28621 gctccggatc gatatagcgc atttcttcgg aagcgaagtt ctgctcatcg cgccgcgggt
    28681 gaatgcagtc gacgggacag gcgtcgacgc aggccgtgtc cttcgttccg atgcagggct
    28741 ccgcaatgat gtatgccatc tgttttcctc agcggatcag tgcggcgcgc tggatgtgtg
    28801 acatgcctgc tgcacaacct ggaagacttc aaatacgtca aacggtttga ccagcacgtc
    28861 gtagcccccc acgttcagca cttcggccca aagccgctca tcggcattcg tctccatcac
    28921 cagaaacacc ggtggatcgg gcgggaaccg caggtggtcc cagagatccc gccaggagcc
    28981 gtccggcaga ttcgcatcgc agatcaccac ccgcggcggt gtacgttcga tctgatccaa
    29041 tgattcctga agcgacgtgg cccagtactg cgggcatgga tgcccctcca gggcggacgc
    29101 gagcgccagc cggtcctcag aggaagaaga gacgatcaag acactattgc tcgccttctg
    29161 actggttgca cagactggac acgagcgtga tgtggatgcg gtttcattcg ccgttgttcg
    29221 agttatcgtt cccatgtctc agtgtgcgga gaggccggcg aagacacaat agaacaggtg
    29281 tcactggcaa cgacgacagg tataacaatc cggcgctaaa cgggtgcgat taaatcaggc
    29341 cccggtgcat tgcattcagt accgcgctca ggcgatcttt cgtgcccagt ttttcgttga
    29401 tgcgatgcag atggttccgc agcgtggatg gagtgatccc cagcgccccc gcgacatcgg
    29461 ccggtgcggc tggagacgcc agcaactgga ggatctgaat ttcccgctct gaaagcgtgg
    29521 gggctggctc cgcaccctgc acgccactgt tcagggcagc tactcttctg gacagacgga
    29581 cccactcgga aatcagctcc tcctgcttct tccggcttgt gatgtcgcgc gccaggtgca
    29641 cgatgatcaa ccggccattg cgcacattcc tgtagaagag cgttgagacg ttcacccaaa
    29701 ccgtcccgcc ggagagagtt cgcgcctcca tgtcgaaatc cggcaccggg ccggcatgca
    29761 gggcacatcg ctgaacaaca cagtcccgca tgcatatggc ggcgccaagc cttccgcggg
    29821 catcgaggat ctcataacaa cgcctgttca ggacctgttc gctcgaaaat ccgaaaagcc
    29881 tctccgccgc cttgttccag gaaagaatct cgccgctctc ggtaaggctg aacgccgcat
    29941 cggccgtcga cgccagcagc gcgaacagct cagcttccag catgacggtc gagctggtcc
    30001 gcgaaaccgg tcagcttccg gatgcgctcc gtaccgatgc gctcgtcgac ttccaggaac
    30061 tgcttcttta cgcgctcgga atcgtcgcct gccagaagct tttccgcgag cggaaatagc
    30121 agattgtcct ctttccagat atggctgttg tacaactccc gcaaagcccg gaggctggcg
    30181 atcagctcct cgcgcgccga tgcggcgttt tcctcgtagg caccgctcgc cctgatcaat
    30241 tcgtcgacga gcgtccgtcc cttgcgatgt tccgccacca ggccgccaag aggacagccc
    30301 gcctgcggca cgcccttcgc ttcaagagca ggaaagagaa tggcttcctc ttttccgtga
    30361 tgtagctcat cggcatagac ccgcaggaat tgcaccagat tctgcaaaac gcttccgtca
    30421 acgccggacc ccgacgtcag ttggtccgcc attttttcca tcaaggcggc ggctttctct
    30481 atcacacgat gttccgcggt gagttcatgg atggggttgg tctgttgcat gatcctgtac
    30541 ctactatgca ccgaaaacgt caagtcatgt tcccgtcaat gttcagcaga gtagtactga
    30601 tggatgcgag caacggttta ctaagtcagt ccacggtggt actctcaaat cgaattttcc
    30661 agtactaaca tggccgaaca ggaggcggca tgcaggatat ctggacggat ctgcaatatc
    30721 gaattattcg ggcgctggct ccgccaagct ctcgcctcga cgagagtagc ttcgagggta
    30781 agagcaagct ccgcgaactg gccggggaca gcattttaga cgagtttcgc ggcaagcaca
    30841 ttatcgaatt cggctgcgga gccggccgtc aaacggtcga gttggcactc gccggggcgg
    30901 ggcgcgtgac cggggtcgac attcaggaag atctgctggt gagcgcccgc gagtacgctc
    30961 aagctgcggg cgttgcggac cggtgcgagt tcgtgacctc gacagaaaag cttgccgatg
    31021 tgatctttac gatggatgca ttcgagcatt tcaccgaccc cgaagcggta ctgcgaaaaa
    31081 tgcgcgcgtc gctcaagccg ggcggctacg tctacgtggt gttcggttat acctggctgc
    31141 accctctcgg cggccatctg ctaagcattt tcccttgggc ccacctgctg ttttcggaaa
    31201 aagctctgat gcgttggcgc gccactgtac gcgatgatgg cgcgacgagg atctgcgagg
    31261 tcaagggtgg acttggcaaa atgacaatcg ggcgcttcga gaggctcgcc gcgtccactg
    31321 gatttcgcgt cgaaaacctg gaactgcgtc cgattcgccg ggctgcctgg ctgcactgcc
    31381 ggctgacgca ggagtttctt acatccggga ttaggtgccg gctcgttccg gtctagcggt
    31441 agcggcgcgg ccactcacaa ctactcgcct ttaagagacc tctccatcaa ctcccgaatg
    31501 gcctctcgcg gcggacgccc gtcgcgaagg atggcgttca tctgttgggc gatgggcatc
    31561 tccacgccgt gaagacgcgc caactccacg gcggcgaacg ttgtctgcac gccctcggct
    31621 accattcgca tcgacgagac gatatcgctc agtgtctggc cgcgggcaag ctcgatgccc
    31681 actcgccgat tgcggctcaa atcgcccgta caggtcagca cgagatcccc gagccccgcc
    31741 agacccgcaa gcgttcgcgg attcccgccg agtgccgtcg cgagcctggt gatttcggcc
    31801 aagccgcggc tgatcagagc tgcccgtgtg ttgctaccca acccaagacc ctggcatatt
    31861 ccggcgccga tcgcgatgac attcttcagc gccgccgcaa tttctacacc aatcgggtcg
    31921 gtgttcgtgt acagccggaa cgacggtccc gaaaacgccg cttgcacggt ttccgccgcc
    31981 tcgcgttccg ccgaagagat gacaacagcg gttggttccc cccgggcaac ctccacggca
    32041 aacgtagggc cggaaagcac tgcgatgcgt ggttcctgct gcccttcgac actctgccgg
    32101 atcacttcgg aaattcgcag cagcgattgt gattccaggc ccttcgttgc gctcacgaaa
    32161 ataacatccg tctgcaccag cgcggccatc tggctgtaga ggcgccgcgc gtggtgcgaa
    32221 ggcatgacgc cgagcacgat ctccgcctct tcgagggcat acgaaagatc ggaagatact
    32281 tcgacgccgt cgggaatgcg gaagtccgga aggaagacat cgttgatccg tgtttccgcc
    32341 atgcgcgcgg cgagatcctg ttcatacacc cacagccgca gccgctggaa tcgcggcgcc
    32401 agaacaaggg caagcgccgt tccccaactg ccgccgccga taatcgccag gttcctcaag
    32461 agcggcggcc tccgaaggaa aacgtgtgct ccttaccggc gcgcagccgt tcgatgttgg
    32521 acttatgccg ccaaacgatg aaggccccgg caaacagcgc cgcggccaga aggggggcgg
    32581 gtggatgcag aataatccac acggcaagcg gaaacgatcc agccgccagt acagagccta
    32641 ctgagatata gcgtgtcacg gccacactca caacgaacac caccagaacg gcagccagcg
    32701 gcaaaggtgt caggtatagg aacgctccga taaagctggc caccgccttc cctccccgga
    32761 acttgaggaa caccggaaac gcgtggcccg ccattacggc aagcgcggcc atgctcatcc
    32821 acagcaggtt gccgttggtc aggcgtgcgg tcagccacac cgcaaagaag ccttttgcaa
    32881 tatcgagcag cagcgtcaca attccaagcg cgcgtccggt ggtccttacg acattggttg
    32941 cgccgatgtt gccgctgcct atcgttcgga cgtcccggcc ggttttccac cgcaccagca
    33001 aatatccgaa cgggatcgca cccacgatgt aggccgcaag aagtacgaga ccgggagtca
    33061 tgcggcgaat tcggcgagct tcgtgtaaac ggagccacca ggcctcaact tgctttcgta
    33121 gagaaagaac cggtctacgg tgaaatttcc gaagtctcgt gtgtcgaggg cggctatcgc
    33181 ctcccgcagt ttctccagtc tcgatcccgg atcgatgcgc gccagcgtca ggtgcggcga
    33241 aaaaggccgc ttctccgcgg cgattcccaa atcctcgcag acgcgttctg tggcggccgc
    33301 gagttctttg agcgatggag gtgcttccac acctgcccag aatacgcgcg gcgacctttc
    33361 gtgaggaaac cacccgagcc cgcgcaccgc tatgggtatg ggtccgggca gcggaattcg
    33421 gcgaagacag gcgatcatct cttccagccg ctcctgcggc cattctccga tgaacttggt
    33481 cgtgatatgg agattaccgg cccggctcca ccgcaaatca gccagcggcc gccatttgga
    33541 aatgagacga tccagccggt cgacgacctc ctgaggaata tcgacagcgg caaacagacg
    33601 catgttttgc taacgtagcc attgtggcac gatgaagccg cctttggcgg cctgttgaaa
    33661 cattcctccg tattggaccg ttctttctat tgccgcggca cgatcgacgt cgcgaaatcg
    33721 ctgcttggaa aagtgctggt gcatggcacg gccgccggag tgatcgtcga aaccgaagcg
    33781 tatctgggcg gagacgatct ggcctcgcac tcatcccggg gcgtcacaaa ccgcactcgc
    33841 gttatcttcg gcccacccgg ccacgcctac gtctacttca tttacggtat gtatgaatgc
    33901 ctgaacctgg tggctgagcc ggagggggtc cctggttgtg tgttgattcg cgcgctggag
    33961 cctgtcgccg gaattgcgat catgcgcgag cgccgtcctc gcgcgaaaaa gcttgaggac
    34021 ctggcttcgg gtcctggcaa actgacgctc gcgatgaata tcacgcggcg cgacaacggg
    34081 cgggatgtca cctgcggccc gctgacggtg cgcagctttc cccaaacaaa gcagcccgaa
    34141 attgttacta cctcccgaat cggcatccgt gaatgcgccg attggccgct gcgatttcac
    34201 atcgcgggga accgctgcgt gagcaggtga ggacgcggtc ctgctacctt acaagtgatg
    34261 ctgaaatcca ccattttccg cgaatacgac attcgaggca ttgccgaaac cgatttgaac
    34321 gacacggccg tggagcaact cggaaagggc attggcacat actttcgcag acgtggcggg
    34381 acaaaggtga atctgggccg ggacacgcgg ctcagctcac cccggcttcg cgatgcgctg
    34441 ctgcgcggct tgctgcaatc ggggtgcaat gttaccgaca tcggagtcgt tccgactccg
    34501 gtactctact actcggcgta caaatggcag cccgacggcg cggtgatgat cacaggcagc
    34561 cacaatccac cggagtacaa cggcttcaag gtggtcagcg gcggctccac catcttcggc
    34621 gacgacatcc aggaaatcta ccggatcatt gaagccggcg cattcgagac aggttccggc
    34681 atggaggatg cggcggacgc gatagggccc tacgtggacg aattggcgtc cctggtttcc
    34741 ttcgacaggc gtattcgcgt tgttctggat gcgggaaacg gcgcggccgg gccggcgatg
    34801 caccggttgc tcgagcgctt gaattgcgat cccgtggagc tgtttttcga aatggatggc
    34861 aacttcccca accaccatcc ggatcccacg cttcccgaaa acctggtggc gctgaaacgc
    34921 gcggtgaagg agcacaaggc ggaactggga atcgcattcg atggcgatgg cgaccgcatc
    34981 ggcgctgtgg atgagaacgg cgaaattatc tggggcgaca tgcttctgct gatttttgcc
    35041 cgcgagattc tcacacgaaa gcccggtgcg acattcattg gcgaggtgaa atgctcgcag
    35101 gtgttttacg acgaggtgcg aaagctgggc ggcaacgcga ttatgtaccg taccggacat
    35161 tcgctgatca aggcaaaaat gaaggaagag cacgccgaac ttgccggaga aatgagcggg
    35221 cacatttttt ttcaggaccg ctacttcggc tacgacgacg cgctatacgc ggcctgccgt
    35281 ctcgtcgaaa tcgtagcccg ttccggcgag ccgctatcgg cacagcttcg cgggttgccg
    35341 aagttggtag ccacacccga gattcgcgtc gattgtcccg atgaagtgaa attcgatctg
    35401 gtgcggcgtg tcgcggaagt gttgcgaaaa tcgcacgaaa ccgtggacgt ggatggcgtg
    35461 cgagtgctat tccccgaagg gtggggcctg gtgcgcgcct cgaacacgca acctatactc
    35521 gtgctccgct tcgaagctac cacccaggag aaattggctc agtaccggaa agaagtggaa
    35581 gacgttctcg aagccgcgaa acaggcagtg gtttgagatc agttccgcac gccgatgctt
    35641 tccgttcgcc ccgccagcgg ttttatcacc gatccgccgc acacacccac gccgacctgc
    35701 atcagggcgt tggcgaaatt atacagcacg gcgtagatca tctgttccgt cacgggctgt
    35761 ttgccgggta gccgaaaacc cgcaggtaat tccttagcga tctgaacgcg gatcgaatgc
    35821 ggcagcaccc ggtcggagcg gtcccgcata ttcgcgagcg caagcggtgt ctgcaggacg
    35881 cggccggtgg agccgtctac gtaaaactgg catcgggaga accatccgag gttgccgggg
    35941 tcggaggcgt caaatagcag cagcggggcc ttgcgctccg ccgccgtcaa ctccagcaca
    36001 atggttttcg gagtctcgtg aagcttgaac tcgaagcccg cctgcttcag gatgctgaaa
    36061 atgatctcct gatccagttc gagcagcaga tacttatgtt ctcctgcttg taaaacctgc
    36121 attgtagcta ttttagcggt ttagcggctc gcgggtgagg aagcctgatc cggaaacccg
    36181 gcggcattta atcccggaat gaccttcaga gcgtttttat agtacagctt cttcagcact
    36241 tcatcgggaa gatccagtcc gtagactttc cagagcccgt gatggggacg gtcatgatcg
    36301 aagtattcgt cggcggtctc caaaattcga aaataggtcg ggtattcctc gggcctccag
    36361 gtatcctttc cgaacattac gcggtcctga tattgcacaa accactggcg ggcgaagcgc
    36421 ggctgcctcc ccagttcctc caccaccgcc gcgatttccg tatagacgtt gggaagacgg
    36481 tccagcagct tgccgagtgc cgcgagatcg cctcccagcc agcccaggtg agcgttgatg
    36541 aacttcgttt tcgggtgccg ggcgaacaag ctgtgctgtt cggccatgat cgcttcccag
    36601 gtcgggtaac ggtctggcgg acggccacgc ctcggatacc ttttcagttc gagccatcgc
    36661 tcgttgtact tgtccatcgg ctcgaagagt cccttgggct ctccggtgtg gatcaggacc
    36721 gggatgttca acttcgcgca catctcgaag atcggatcga aacgcggatc gtcggtgcgg
    36781 atgcgctcac ccttgccgtc tttaaggtcc aggccgagat tcttgaaaat tttcagccct
    36841 tgcgcgccgt tgcggacgtc ctgttccagt tgcgccgcgg cacgcttgcc gtaatccggt
    36901 tcgtcgatgc ccgtaaagtc gatgttcgag aagacggcaa agcggtcctt cgacgcgctc
    36961 ttcatcgcct gaacggtttg ggccagccgg tcgccatagc caccgctcag gttcaccatg
    37021 attcgcatgt tgatggaatc catgtcggcc agcaactgac gaagacgctc cggcgacagg
    37081 cttctctgat gattgtgcac gtcgatgaaa gggaacctgg ccttcggcac ccggtgttgt
    37141 ggaaccacca gccccgattt cggactgtat tcctcgatcg acagagattg cgcccccagg
    37201 atcccaggca gagcaaatgc cagcagaagc gaaaagatt
//
LOCUS       DASTMK010000042         8589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_226180, whole genome shotgun sequence.
ACCESSION   DASTMK010000042 DASTMK010000000
VERSION     DASTMK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226180"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFQ79_01730"
     CDS             complement(<1..307)
                     /locus_tag="VFQ79_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138394.1"
                     /translation="MRTLLCFLMLASVVPMWGVGTPTRQVPVEITHEKGSFSGQLAVS
                     LNGNMNKAKAEWNATIRNTSANKIFRVTFCVKAFDAADQQIKAGGNECVLTLWGSNWQ
                     "
     gene            complement(327..854)
                     /locus_tag="VFQ79_01735"
     CDS             complement(327..854)
                     /locus_tag="VFQ79_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138395.1"
                     /translation="MKGKILFGVLALSFVSMVSAQQNIFTNIGVLQKAAVPILSSVGW
                     KDLGSGVGFYGSSQIELPTSGFVPSTVRYSISGKTGNRADQALISAFIASPRDLSAGR
                     AKVEAAATQWFKSVGKPVPAGLIAAIKTGKPFQSTAGGLSLQYVVERGMGSPIKQPDG
                     TSYRCTTMDLTIKPL"
     gene            complement(917..1147)
                     /locus_tag="VFQ79_01740"
                     /pseudo
     CDS             complement(917..1147)
                     /locus_tag="VFQ79_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_052362288.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1128..1424)
                     /locus_tag="VFQ79_01745"
     CDS             complement(1128..1424)
                     /locus_tag="VFQ79_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138396.1"
                     /translation="MLAWHLGKRTRIVTEGFISKLRWATADALFQLTTDGFQPYIGAV
                     DAGLSDRVDFVQLVKVVRHEPGRRSPGTLHQMLWMLSQLSSWGILTEADLHVAH"
     gene            1537..2937
                     /locus_tag="VFQ79_01750"
     CDS             1537..2937
                     /locus_tag="VFQ79_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138397.1"
                     /translation="MSDSKPGVTSRRRFLTDAGRTAGASALAGVALPHVHAAENNTIQ
                     IALVGCGGRGTGAAINALSVTNGPTKIVAMADVVPKKLSDSYDNMTSQFADQVDVPPE
                     RRFLGFDAFRSAMDALKTGDLVILGTPPAFRWVQFSYAIDKGLHCFMEKPVTVDGPTS
                     RRMFQLGELSVQKNLKVGVGLMVRHCRARQELLDRIRSGQIGDIISMRAYRMGQGGGT
                     ASPRPEGIGELAYQVQRFHAFLWASGGVFSDYYIHQIDECSWMKGAWPVEAHALGGRH
                     YRGDSLDQNFDTYSVQYTYPDGTRLFFDGRNMAGCRDEFASYAHGSKGSAVISTLVHT
                     PGMTRIYKGHNIPKVSNRSELPLPENPNLVWAFPQPERSPYQYELDDLMDAIRNDKPY
                     NEVRRGTEASLVTSMGRMAAHTGQIVTYDQILNCPHEFAPDVDKLTAESPAPLQMGPD
                     GKYPMPEPGIKKDREY"
     gene            complement(2943..4022)
                     /gene="tsaD"
                     /locus_tag="VFQ79_01755"
     CDS             complement(2943..4022)
                     /gene="tsaD"
                     /locus_tag="VFQ79_01755"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU0138398.1"
                     /translation="MRILGIESSCDETAAAVVENGTTVLSSIVASQLSTHAKYGGVVP
                     ELASREHLRAIVPVVRLALEESNTALPELDAVAVTEGPGLVGALLVGITYAKALCFAR
                     NIPLIAVNHIEGHIFAVVMDAPREGEAIDFPAVALVVSGGHTHLFEVLEGFRYRLLGR
                     TRDDAAGEAFDKVAKLLGFGYPGGPVIDRLAEFGNPRAVRFTSPKMKGNPLDFSFSGL
                     KTAILRWFEARDMADEVEARRRLLSRTLTPTTKEWLAVTPKETLDLLASFQRTVVDEL
                     LRRARATAEQTGAHALIISGGVACNSGLRQAAAQAGFPYPVYFPTPKLATDNAAMIAA
                     AAFPKYQRGEFAEMSLKARANLALA"
     gene            4064..5119
                     /gene="queA"
                     /locus_tag="VFQ79_01760"
     CDS             4064..5119
                     /gene="queA"
                     /locus_tag="VFQ79_01760"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU0138399.1"
                     /translation="MDVSEFDYHLPEELIAQRPLDDRAASRMLVLHRGTASWEDRMFR
                     DLPGFLSPGDCLVLNDSKVFPSRLFGHRVGTHSLPIGKNNPKRWQYLTGRVEVFLLRP
                     ISEDRKTWEALVRPGRKMQVGERIRFEGDLEAEIIGRGEFGERTIRFEDAGDIYDALD
                     RIGHVPLPPYIRRPDSPEDRERYQTVFAREAGSVAAPTAGLHFTPEMLEKCRTAGTSI
                     VTVTLHVGLGTFQPIHTGVVERHSIHSEHFEISENAWCQIESASRVVAIGTTSVRSIE
                     TAARGKQSGETGLFIYPGFRFTVTGAMLTNFHLPQSSLLVLVAAFAGHEFVMAAYRHA
                     VEQRYRFYSYGDCMLIL"
     gene            complement(5126..5566)
                     /locus_tag="VFQ79_01765"
     CDS             complement(5126..5566)
                     /locus_tag="VFQ79_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138400.1"
                     /translation="MRARIPVVALRQWLNKLRNKPDVPLRGAPPVRRQKTYSSRSGYV
                     YQYFYLGQRPALRSAEQGTEYVFEVTVDRKISFPVPVFLTSQAVKSWNDSHGRTLTGT
                     EEYAIAKMALFQAFDERTHPGQMKHEIRVRPADVAGILEMLDID"
     gene            5635..6531
                     /locus_tag="VFQ79_01770"
     CDS             5635..6531
                     /locus_tag="VFQ79_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138401.1"
                     /translation="MKLSVLVTALGMFAVDLFPQAAPPDRLQNTGKPMRLPFECSEEQ
                     VRTLGLSCSASDPCPVYLELSEAAVAGAKVFVTGNIHNTSTTISSVLLASEDGGATWF
                     EPHERIMSAGLERIQFLDPNSGWAGGQLLLATPRDPFFLLTTDGGTTWRKRAVFGESR
                     IGIIEQFWFDTRTSGMLLIDRLHTGENGARHELYESMTGGDSWMLKQVSTRPIPVKRI
                     EVSQPAIRIRADAPSKSYHVERRQGDGWRTIARFLVQIGECKPEHQDMPPPPPPEEPG
                     VPEPSPSKAAPRTGSPPSLRKP"
     gene            6606..7004
                     /locus_tag="VFQ79_01775"
     CDS             6606..7004
                     /locus_tag="VFQ79_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0138402.1"
                     /translation="MSTVVANPLAAQNVQNRRKAAFMVFGCTIFGAFAQILFKTGLRL
                     MPQVTPLAVMTNVPLLAGLTLYGISTLLLVMALREGELSLLYPVISLTYAWVTVLSVF
                     WLGETLNVFKVVGILIIVSGVAMLGRTRSS"
     gene            7001..7351
                     /locus_tag="VFQ79_01780"
     CDS             7001..7351
                     /locus_tag="VFQ79_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0138403.1"
                     /translation="MTTPVSSMVLVLIGSFIGSFGAVFLKSGAGRTNCLKTLLFNPRL
                     VAGVTLFLLSTVFYLSGIREGELTILYPMVSLGYVWTLLWSRLFFKEPLTRNKFLGLG
                     MILAGIIVLSFGNR"
     gene            complement(7361..>8589)
                     /locus_tag="VFQ79_01785"
     CDS             complement(7361..>8589)
                     /locus_tag="VFQ79_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0138404.1"
                     /translation="AREGVTFENNFCASAICMTSRASIFTGLHERTHGISSFTRDFTP
                     QQLSATYPQILREAGYRTGFIGKYGVGSNLPAAHFDYFKGFPGQGRYYHERNGKTVHL
                     TQILGEQAAEFLESSSDHQPFCLSVSFKAPHVQDGDPRQFLYDPVHEGLYRSDSIPLP
                     KTADERYFEALPSFLKNSEGRERWKIRFATPEMYQRSVKGYYRLITGVDAVAGSLLKL
                     LETRRLLDNTVVVFTSDNGFFLGEHGLAGKWLMHEESIRTPLIIRDPRLRSSRGGSRV
                     KRPALNVDIAPTLLGLAGLEPPRAMQGRNLAPLLAGTDVAWRRDWYYSHLFQHPKIPR
                     SEGLRNERWKYIRYLDTKPVYEELYDLESDPLEEHNLTLAKQQPAVLAGMRSRLVTWR
                     RRLGEWDPVQRWQEPS"
BASE COUNT         1736 a         2599 c         2450 g         1804 t
ORIGIN      
        1 gttgccagtt acttccccac agggtcaata cgcactcgtt tccaccggcc ttgatttgct
       61 gatcggcggc gtcgaacgcc ttcacgcaaa acgtcacccg gaaaatcttg ttcgctgacg
      121 tgttccgaat cgtggcgttc cattcggcct tcgccttgtt catgttaccg ttcagcgaaa
      181 cggccaactg accgctgaag ctcccctttt cgtgcgtgat ctccacaggc acttgccggg
      241 ttggggtgcc aacgccccac atcggcacaa cactggcaag catcaaaaag caaagtaagg
      301 ttctcatgat gattcgacct ttcaagctat agcggcttaa ttgtcaagtc catcgtcgtg
      361 catcggtacg atgtgccatc tggttgcttg atcgggcttc ccatcccccg ctccacgaca
      421 tactgaagcg ataaaccgcc tgcggtgctt tgaaagggtt tgcctgtctt gattgccgca
      481 atcagaccag ccggaacagg cttgcccacg gatttgaacc attgcgtggc cgccgcttca
      541 accttcgctc tcccggcact caaatctcgt ggcgaagcaa tgaaagccga gatgagagct
      601 tgatcggccc gattcccggt cttcccagag atagagtacc taaccgtcga tggcacgaat
      661 ccactggtag gtagttcaat ctggctcgaa ccgtagaacc cgactccaga gccaaggtct
      721 ttccacccga cactggacaa gattggcaca gcggcctttt ggagaacgcc gatgttcgtg
      781 aagatgttct gttgcgccga taccatcgac acgaaactga gcgcgagcac gccaaacagg
      841 atttttcctt tcatttctta cctttcccgc atatctgtga agcaggtata gacagggtat
      901 cagaatttga gacccattaa accagtagtt caccaagccc ccaaacgtga tccgtgattc
      961 ccgccgccat cgcggaggtg accctcagcg tcgtatggac ccggcagaag ttgtagtagg
     1021 tgaaatgtag tgccagtgcc gctctcaagt tctcccactt ctttgagaac gcattcgtta
     1081 atcgggtgag ctgccgaata cacattcgca tcgtcaagtt ctgccgttca atgtgcgacg
     1141 tgcagatcgg cttcggtcag gattccccag gatgacagtt gggacagcat ccacaacatc
     1201 tggtggagag taccggggct tcgtcttccc ggttcgtggc gtacaacctt cacgagttgt
     1261 acaaagtcca cccgatccga aagccccgca tccaccgccc cgatataggg ctggaagccg
     1321 tcggtcgtca actggaacag cgcatcggcg gttgcccatc gcaacttgct gatgaagcct
     1381 tccgttacaa tccgggtccg cttgccgaga tgccacgcca gcaccagctt tgtgatgcat
     1441 ttgatggcgc cacggtattt aggacactac caactgaccg gcgcggcgtc ccgccttgtt
     1501 cgctgaggta agcttacagg tcagggagag attgccatga gtgacagcaa acccggcgtc
     1561 acgtcgcgac gccggtttct gacggatgcc ggccgtacag cgggagcctc agccctggcg
     1621 ggggttgcac ttccgcacgt acatgcggct gagaacaata ccattcaaat cgcgctcgtg
     1681 ggctgcggtg gccgcggaac gggcgcggct atcaacgcgc tttcggtcac gaacggtccc
     1741 acgaaaattg tggccatggc ggatgtcgtt ccaaaaaaac tcagcgacag ctacgacaac
     1801 atgacgtcgc agtttgccga ccaggtggac gtaccgcccg agcgcaggtt cctcggcttc
     1861 gatgcattcc gttcggcgat ggatgcgctg aagaccggcg atctcgtcat cctgggtacc
     1921 cctcccgcgt tccggtgggt gcagttcagc tatgcgatcg acaaagggct gcactgcttc
     1981 atggagaaac ccgtcaccgt cgacggccca acaagccgca ggatgtttca actgggcgag
     2041 ctttcggtgc agaagaacct gaaagtcggc gtgggtctga tggtccgcca ctgccgggct
     2101 cgccaggagc tcctggaccg gatccgctcg ggccagatcg gtgacatcat atcgatgcgc
     2161 gcgtatcgca tgggacaggg gggcggcacg gcttctccac ggcccgaggg catcggcgaa
     2221 ctggcgtacc aggtgcaaag gtttcatgcc ttcctgtggg ccagcggcgg cgtattcagc
     2281 gactactaca ttcaccagat cgatgaatgc agttggatga agggcgcctg gcccgtggaa
     2341 gcgcacgcgc tcggcgggcg gcattatcgc ggggactcgc tggaccagaa cttcgatacc
     2401 tactcggtgc agtacaccta tccggacgga acccggcttt tcttcgacgg gcgtaacatg
     2461 gccgggtgcc gtgatgagtt cgccagctat gcgcacgggt cgaagggttc ggctgttatc
     2521 tcgacactgg tacacacgcc gggaatgacg cgcatctaca agggccacaa cataccgaag
     2581 gtctccaacc ggagtgaact gcctctgccc gagaatccga acctcgtctg ggcattcccg
     2641 caacccgagc ggagtccgta tcagtacgag ttggacgacc tgatggacgc cattcgaaac
     2701 gacaagccct acaacgaagt gcggcgcggc accgaagcaa gcctggttac gtctatgggc
     2761 cgcatggcag cccatacggg ccagattgtc acctacgacc agatactcaa ctgcccgcac
     2821 gagtttgcgc ctgacgtgga caaactcacg gcagagagtc cggcgccctt gcaaatggga
     2881 ccggacggaa agtacccgat gcccgaacca ggcatcaaaa aagaccgcga gtactgaggc
     2941 gttcaggcca gcgcgagatt ggcgcgcgcc ttcaaagaca tctccgcgaa ctctccgcgc
     3001 tgatacttgg gaaatgccgc ggcggcgatc atcgcggcat tgtcagtcgc aagcttcggc
     3061 gtggggaaat atacgggata aggaaatccg gcctgtgccg ccgcctgccg gagaccggaa
     3121 ttgcaggcta cgccgccgga aatgatcagc gcatgcgcac ccgtctgttc ggccgtcgcc
     3181 cgggcgcggc gcagcagctc gtctacaacc gttcgctgaa acgacgcaag cagatcgaga
     3241 gtttccttcg gagttaccgc aagccattct ttggtcgtcg gcgtcagcgt tctggacagc
     3301 agtctccggc gcgcttccac ttcgtccgcc atatcccgcg cttcaaacca acgcagaatg
     3361 gcggtcttca atccgctgaa gctgaaatcc aacggattgc ctttcatttt gggcgaggtg
     3421 aaccggacgg cgcgcggatt cccgaattcc gccagccggt cgatcaccgg accgcccgga
     3481 tagccgaacc ccagcagctt cgccaccttg tcgaacgctt cccccgcagc gtcgtcgcgt
     3541 gtacgcccga gcagccggta ccggaatcct tccagcacct caaacagatg agtgtgcccg
     3601 ccgcttacca ccagcgcgac cgccggaaag tcgatggcct caccctcccg gggggcatcc
     3661 atcaccacgg cgaaaatatg gccctcgatg tggttgaccg caatcaacgg gatattgcgc
     3721 gcaaaacata aagccttggc gtaggtgatg cccaccagca gcgcgcccac caacccgggt
     3781 ccctcagtca cggctaccgc atccagctcc gggagagccg tgttcgattc ttcgagcgcc
     3841 agccgaacca ccgggacgat ggcgcgcaga tgctcgcgcg acgccaactc cggcacgaca
     3901 ccgccatatt ttgcgtgcgt ggataattgg gaggccacga tcgaagagag aaccgtggtc
     3961 ccgttttcca ccaccgccgc cgcggtttcg tcacacgaac tttcgatgcc aagtatgcgc
     4021 aataccgttg cctgcctgcc cgcgaatgag acaatcgtgt tgcgtggatg tctctgaatt
     4081 cgattatcac ctgccggaag agctgatcgc gcaacgcccg ctcgatgacc gcgccgcatc
     4141 cagaatgctc gttctccatc gcggcaccgc gagttgggag gacaggatgt tccgggacct
     4201 tccgggattc ctctctccgg gcgattgcct ggttctgaac gactccaagg tttttccctc
     4261 ccgccttttc ggtcatcgcg tgggaacaca ttcgcttcct atcggcaaga acaaccccaa
     4321 gcgatggcag tatctgacgg gacgtgtcga ggtttttctg cttcgcccga tctctgaaga
     4381 ccggaaaaca tgggaagcgc tggttcggcc cggccgcaaa atgcaggtcg gagaacgcat
     4441 tcgctttgag ggcgatctcg aagccgaaat catcggccgt ggtgaatttg gagaacgcac
     4501 gatccggttc gaagacgccg gcgatatcta tgacgcgctg gatcgcatag gccatgtgcc
     4561 attgccgccg tatatccggc gtccggattc gccggaggac cgcgaacgtt atcagaccgt
     4621 attcgcacgg gaagcgggct cggtcgccgc gccgacggcc ggccttcact tcacgccgga
     4681 gatgttggag aaatgccgta cggccggcac ttctatcgtc accgtgacgc ttcatgttgg
     4741 actcggcacg tttcagccga tccacaccgg ggtagtggag cgccactcca ttcactccga
     4801 gcacttcgag atttcagaga atgcatggtg ccagatcgaa tccgcctcgc gcgtggttgc
     4861 catcggaacc accagtgtcc ggagcatcga aacggcggcc cggggaaaac agagcggcga
     4921 aaccggcctg ttcatctatc ccggcttccg gttcaccgtc accggagcga tgctcaccaa
     4981 cttccacctt ccccaatcga gcctgctcgt tctggtagcg gccttcgccg gacacgagtt
     5041 cgtgatggcg gcgtaccgcc acgccgtcga acagcggtac cgcttctatt cctacggcga
     5101 ctgcatgctg attctgtgat ggaggctaat cgatatcgag catttccagg atgccggcta
     5161 catccgccgg ccggactcgt atttcgtgct tcatctggcc cgggtgcgtt cgttcgtcaa
     5221 acgcctgaaa caaggccatt tttgcgatcg cgtattcttc cgttccggtg agggtacggc
     5281 cgtgggagtc attccacgat ttcaccgcct ggctggtcag gaaaaccggc acgggaaaag
     5341 agatcttacg gtccaccgtt acttcaaaca cgtactcggt accctgctca gcgctgcgga
     5401 gagcaggccg ctgccccagg taaaagtact gatagacata gccgctacgg ctggagtacg
     5461 tcttctggcg gcgtacgggc ggcgcgccgc gcagcggaac atccggtttg tttctcagct
     5521 tgttcagcca ttgccgcaac gccacgacag ggattctagc acgcattgcg gaccgggatt
     5581 ccaccggccg gcctttcaaa gcgctcatcg ggtgagctag aatcgcattg cggtatgaag
     5641 ctgagtgttc tggtgacagc tttagggatg tttgcggttg atctgttccc gcaggccgcg
     5701 ccgccggacc gcttgcagaa caccggcaag ccgatgcggc taccattcga atgctcggag
     5761 gaacaggtgc ggacgctcgg cctcagttgc tccgcctccg acccgtgtcc cgtgtacctg
     5821 gaactctccg aagcggcggt tgccggagcg aaggtcttcg tcacgggcaa catccataat
     5881 acatccacca ccatcagttc cgtgctgctg gcgagcgaag acggcggtgc cacctggttt
     5941 gagccgcatg agcgcataat gtccgccggg ctcgaaagga tccagtttct cgaccccaat
     6001 agcggctggg caggcggtca actgctgctg gcgacaccgc gcgatccttt ttttctgctg
     6061 actacggatg gtggaacgac ctggagaaag cgcgccgtat tcggcgagag ccgcatcggc
     6121 atcatcgagc agttctggtt cgacacacgc accagcggaa tgctgttgat cgaccggctt
     6181 cataccggag agaatggtgc gcgccacgag ctgtacgaat ccatgacggg cggggattcg
     6241 tggatgctga agcaagtgag tacccggccg atcccggtga aacgcattga ggtttcgcag
     6301 cctgcaattc gcatacgcgc ggacgcccct tcgaagtcgt atcacgtaga gcggcgccag
     6361 ggcgacggat ggcgaaccat cgcccgattc ctcgttcaaa tcggagaatg caaaccggag
     6421 caccaggata tgccacctcc cccaccgccc gaggaacctg gagtaccaga accgtctcca
     6481 tccaaggcgg cgcctcgtac cggttcgccg ccgagtttac gaaaacccta gctggctcag
     6541 ccggtggaaa ggtacagcag aaatatcgcc gcagttgcta ttatggagga cgcttgggag
     6601 atccagtgag tacagtcgtc gccaatccat tggcggctca gaacgtgcaa aatcgccgga
     6661 aggccgcttt catggtcttc ggctgtacaa tcttcggagc tttcgcccag atccttttca
     6721 agaccggttt gcgtttgatg ccgcaggtta cgccactcgc cgtgatgacg aatgtgccgc
     6781 ttctcgctgg acttactctc tatggaatca gcactttgct gcttgtcatg gccctgcggg
     6841 agggtgagtt atcgcttctc tacccggtta tttcgctcac atatgcctgg gtgacggtgc
     6901 tttcggtttt ctggcttgga gaaaccctga acgtgttcaa ggtggttggc atattgatta
     6961 tcgtttcggg tgtagctatg ttggggagga ccagatcgtc gtgaccaccc ctgtctcttc
     7021 gatggtcctg gtgctgattg gatctttcat cggctctttc ggcgccgtat ttctgaaatc
     7081 gggcgcggga agaacgaatt gcctcaaaac gctgttgttt aaccctaggc tcgtcgcggg
     7141 cgtgacgctt tttcttctgt ctacggtgtt ttatctctcc ggcatccggg aaggagaact
     7201 gacgatcctc tacccgatgg tgtcgctggg gtacgtatgg acgctgctgt ggtcgcgcct
     7261 tttctttaaa gagccgctga cgcggaacaa gttcctcggc ctgggaatga tcctcgccgg
     7321 aattatagtc ttgagtttcg gcaatcgctg agttccgtac tcacgacggc tcctgccagc
     7381 gttgtacggg gtcccattcg cccaatcgcc gccgccaggt taccaggcgt gagcgcatgc
     7441 cggccagcac agccggctgt tgtttcgcga gtgtcaggtt gtgttcttcc aggggatcgc
     7501 tttcgagatc gtacaactcc tcatataccg gttttgtgtc gagatagcgg atatatttcc
     7561 aacgttcgtt acgtagccct tcactcctcg gaatcttcgg atgttggaac aggtgtgagt
     7621 aataccagtc gcgccgccag gccacgtccg ttcccgccag gagcggcgca agattccggc
     7681 cctgcatcgc ccggggcggc tccagcccag ccaggccgag cagagtggga gcgatgtcca
     7741 cattcaacgc cggccttttt acccggctgc caccccgtga ggacctcaga cgcgggtccc
     7801 ggatgatcag cggagtcctg atggattcct catgcatcag ccatttgccc gccagcccgt
     7861 gctcgcccag gaaaaagccg ttatcggacg tgaagaccac aaccgtgttg tccagcagac
     7921 gccttgtttc gagcagcttc aacaggcttc cggcgaccgc gtccaccccc gtaatgagcc
     7981 ggtaatatcc ctttaccgat cgctggtaca tctccggcgt ggcaaaacgt attttccaac
     8041 gttccctgcc ctcggagttc ttcaaaaacg aaggcaatgc ctcaaaatac cgttcatccg
     8101 ccgtcttggg cagggggatg gaatcggagc ggtacaggcc ctcgtgaacg ggatcataga
     8161 gaaactggcg cgggtcaccg tcttgaacat ggggagcttt gaaactcacg gacaggcaga
     8221 acggctgatg gtctgaagaa ctttcgagaa actccgccgc ctgctccccc agaatttgcg
     8281 tcaggtgaac cgtctttcca ttccgctcgt gataataccg gccttgtccc ggaaagcctt
     8341 tgaaataatc gaagtgcgct gccggcaggt tgctgcccac cccgtattta cctatgaacc
     8401 ctgtacggta ccccgcctcg cggaggatct gcggatacgt cgcgcttaac tgctgcggtg
     8461 tgaaatcccg cgtgaaactg ctgatcccgt gtgttcgttc atgcaggccg gtaaagatgc
     8521 tggcccggct ggtcatgcaa atggccgacg cacagaagtt gttttcgaac gtcactcctt
     8581 cccgggcca
//
LOCUS       DASTMK010000043        12118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_610850, whole genome shotgun sequence.
ACCESSION   DASTMK010000043 DASTMK010000000
VERSION     DASTMK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610850"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1297
                     /locus_tag="VFQ79_01790"
     CDS             188..1297
                     /locus_tag="VFQ79_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0138405.1"
                     /translation="MRNANKPASIKDIARIARVSHSTVSRALRESPLVNPETAEKIRR
                     IAAENGFRTSAIARSLATSRTSTIGVVVTSIADPFVAEVVSGIESEAIAHNYSVFLAN
                     CNADPGRELKVVHSFEDRRVDGIVVTASRVGALYGSLLEQMQIPIVLLNNQHPSHFAH
                     SVLIENFEASRGAVNLLVELGHRRIAYIGDRFGYSSDSERFSGYRSALDEADIPFQPD
                     LVVHGDGKAEGGSEAMERLLAAPERPTAVFCYNDMTAIGGLKAIRAHGLKVPDDISLV
                     GFDDLPLALYVDPPLTTVRQPKFDMGRLAMQVLLKLIAGVDAEQDVKVTGKLIVRQST
                     APPKEKTECNYSYAPKTGRKRKRMAFSSSRSATAN"
     gene            1213..2037
                     /gene="iolB"
                     /locus_tag="VFQ79_01795"
     CDS             1213..2037
                     /gene="iolB"
                     /locus_tag="VFQ79_01795"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEU0138406.1"
                     /translation="MQLLVRPQNGTQAETHGFQFLAFSNRKLRPGERVEADTADRELG
                     LVLLGGTCSVASSRGKWTGIGRRPHVFAGMPHALYLPVATRYTVMADTECDLAFCYCK
                     AEEYHPPALITPAEIEVEIRGAGNATRQINKIIRPEFPAHRLLVVEVYTPGGNWSSYP
                     PHKHDVHNPPGEVDLEEIYYYRVDRTEGYAIQKVYTPDRRLDETITVRDGEMVLVPEG
                     YHPVVAAHGYNVYYLNALAGSARSMAASDDPDYIWVRDTWRDKDPRVPLVTMEMEN"
     gene            2057..3934
                     /gene="iolD"
                     /locus_tag="VFQ79_01800"
     CDS             2057..3934
                     /gene="iolD"
                     /locus_tag="VFQ79_01800"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEU0138407.1"
                     /translation="MRTQRLTTAQALVRFLKNQYVERDGREHLFFHGAWGIFGHGCIA
                     GLGQALQENPDFRYYQSKNEQASVHIATAFAKTRNRLGTFACISSIGPGATNMITGAA
                     TATINHIPVLLLPGDLFARRNVAPVLQQLESEHSQDVSVNDAFKPVSRYWDRINRPDQ
                     LICSVVEAMRVLTSPAQTGAVTLALPQDVQSEAWDYPEEMFEKRVWHIPRAEPDRAIL
                     ARAAERIRSSKRPLVVAGGGVLYSDATEALAQFAAQTGIPVGETQAGKGSLPFDHPQN
                     LGAIGVTGTPGANIAARDADLVIAVGTRLSDFTTASKTAFQNPDVQFININVAEFDAF
                     KHAGLPLVADARVTLEALGAALAGYRVQDDFANCIPQWKDRWEKETDRIYGLRHGPPI
                     SQGEVIGAVNRVSRPQDIVVCAAGSLPGDLHKLWRTRQPNGYHLEYGYSCMGYEIAGG
                     LGVKMADPSREVYVMVGDGSFLMMSSEIATSVQEGYKLNIVVLDNHGFSSIGGLSQAI
                     GSHGFGTDYRHRTESGQLDGEHVPVDFAALCQGLGARTVRARTREELENALEAMKRDD
                     RTTAVVIEVDKEMRVPGYESWWDVPISEVSRIESIRAARAAYEEALKRERRHEVAAGA
                     R"
     gene            3939..5429
                     /locus_tag="VFQ79_01805"
     CDS             3939..5429
                     /locus_tag="VFQ79_01805"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0138408.1"
                     /translation="MTGTNELRTDVLDYIGGTWRRPSSDAVTEVLNPATGDVIARAPA
                     GSAQDVEAAVEAASAAFPEWRSTPPADRIQYLFRLKQLLDDHFEEIARITTTENGKTL
                     SEARGELRRGIENVEVACGIPILMQGYNLEDVASGIDELMFRQPLGVVAAITPFNFPS
                     MIPLWFLPYAIACGNTFVLKPSERVPFSMIRIAELIEQTGLPKGVVNIVNGGKNAVDA
                     LLDHPSVQAVSFVGSTAVARYIYSRASAAGKRVQCQGGAKNHVVVLPDADRETTTRII
                     ADSAYGCAGQRCLAVSVAVTVGEAGEWFPKSITETAAALKVGSGLEDGVQMGPVITPA
                     SRERIRSLIAQGASDGARPILDGRGISLSEWPRGNFVGPTVLDGVPAASSLAETEIFG
                     PVLSVIHASGVDEAMEIIARNSYGNASSIFTTSGAAARKFRNQIPTGNVGVNIGVAAP
                     MAYFPFSGWKGSFFGVLHAQGRDAVEFYTDKKVVIERWPKEWSRRF"
     gene            5429..6376
                     /locus_tag="VFQ79_01810"
     CDS             5429..6376
                     /locus_tag="VFQ79_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0138409.1"
                     /translation="MSTAVGSNVAMLRVGNAPCSWGTLEFEEAKGEQIAFDRMLDELA
                     ETGYTGTELGDWGYMPTDAAVLKAELDKRGLVMLGAFVPVALKDPAAHEPGIANALKT
                     ARLLAAVSAEPKPYLVLADNNGTVPERTRNAGRITPEMGLNAEEWRTFAGGANRLGRV
                     VLEETGLRTVFHHHCAGYVETPDEIARFLELTDPGAIGLVFDTGHYAYGAGHCDVLQG
                     LERYKERVLYMHLKDCQPEVARQARINEWDYFEALRHGVFCELGQGGVDFPAVLQWLK
                     KNSYAGYTLVEQDVLPGIGAPKESARRNREYLRSIERAL"
     gene            6373..7422
                     /gene="iolG"
                     /locus_tag="VFQ79_01815"
     CDS             6373..7422
                     /gene="iolG"
                     /locus_tag="VFQ79_01815"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEU0138410.1"
                     /translation="MNKQLNFGLIGAGRIGKVHAETIAFRIPEAALTAVSDIDAEAAD
                     AVARCCGTPRVAASAAEIFADPAIDAVMICSSTNTHADLIIEAAQAGKHIFCEKPIDH
                     SLEKIDRALDAVNRAGVKLQIGFNRRFDPNFARVRRSIATGEIGTPHLLHIVSRDPGP
                     PPLAYIKVSGGLFLDMTIHDFDMARFLVGSEVTEVYAAAGVRVDPDIGKAGDVDTAVI
                     VLRFANGVIGTIDNCRQAPYGYDQRVEVLGGLGAIATENCYPNRAVISTAESVRRDLP
                     LNFFMERYAESFANELQAFVEAIVHDQQTPVTGLDGRVPVVMALAARKSHEEGRPVLL
                     DEIAAPEARLETISH"
     gene            7594..8796
                     /locus_tag="VFQ79_01820"
     CDS             7594..8796
                     /locus_tag="VFQ79_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEU0138411.1"
                     /translation="MAEAPTKLVTVGYSPFLISLGMVAGVFVARWTVPVALKGTTYIW
                     ADLREKPHLIVWAVLAGMLWAVANTLTIFAIRNVGLSIAFPLWNTNSLVGLFWGWLLF
                     NELRGAGGRQRAKVLGGAAAIVTGACLLAYATSHQSSSGPAGAATLGVIAAIGAGVLW
                     GTMYIPYRKAYISGMNPLSFVTVFTFGELGTVFTLAVVFDGGVQNVIVALGHARPALF
                     WLFLGGFCWVLGDLFQQYAAKYIGISRGIPLSNTNQLWGLAWGALVFGEFAAHGFAAQ
                     LLIVAGSLVMIAGAAAISTAEAPESEQRSWRDAMERECDRYGLDRDRVASTMQGEDPL
                     AAERRPRRWWELVIVAGAVALFVWLAAGAERQPLDVSIPWMAALVVASLVFLIACGLL
                     LWKRTRFS"
     gene            complement(8818..10170)
                     /locus_tag="VFQ79_01825"
     CDS             complement(8818..10170)
                     /locus_tag="VFQ79_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138412.1"
                     /translation="MKDDLTKSSRRDLLRFASLTTLGAGLSQGQQPKAQGDTVYEMKF
                     EPKKSVRMGFIGLGGRGTGLMRNFAAVDGVEVQALCDIDKERLPRAQAILEKLGHSKV
                     PTYYHQGERAFEELAKRDDIDLVCVGTPWNWHVPMAAYAMKQGKHVAVEVPAATTIEE
                     CWELVNTSEKTRRHCVMLENCCYGYNELLVLNMVRAGVFGDLTHGACAYNHDLRDLLF
                     SDRGEGLWRRTDHIKRNGNLYPTHGLGPVANYMDINRGDKFESMVSMSSPAISLAYFR
                     DQKVPANDPRRKETYRCGDMNISLIKTTKGRLITLEHNVTSPQPYDRINLISGVKGIF
                     RDYPPRIYLDSMGKHEWGSIDSFKAQYEHPYWKELGETARKLGGHGGMDFIECYRLIQ
                     CMKEGIVPDMDVYDAAAWSAPGPLSDLSVAKNSAPVPFPDFTRGRWKETSRAFGKQTG
                     "
     gene            complement(10242..11678)
                     /gene="csb2"
                     /locus_tag="VFQ79_01830"
     CDS             complement(10242..11678)
                     /gene="csb2"
                     /locus_tag="VFQ79_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HEU0138413.1"
                     /translation="MPTAIALKFPSGHYHATAWGRHVNEGSVEWPPFPWRILRAIVAA
                     WKVRTDGSDAVLESVVQKLATPPEFYLPPATIGHARHYMPLYRGNRTLVFDTFAVLDR
                     NTELQVVWPDVDLTQEEALSLDAALRQLTYLGRAESWCEARLMERSTAATNCHPPRPG
                     MAGADVDTEAVRVLTVDSARAIAQGTAPEAWPLCRTTLDLRNERWSDPPGSAWVTYWR
                     RLDCFAGGASRRIEKPSKKYTVARFALYSRVRPLVTETIAIGEFACQELQGCYGLLHS
                     GTASPVFSGRDSDGNPRQDHNHAFFLPTDEDRDGRIDHLTVYAEVGFGDGELRALDRF
                     REFRQVGKNRPDIRPVLIALGTETDVGNHHLFRKSTKWESITPYVPVRHTKRRGGIWV
                     DTAADQLCRELEKRGIGTMPRVIAQYERDMRGRRWVEYRLERLLGGGTHGQLYGNGFV
                     VQFTEPVAGPISLGYGCHFGLGQFRALE"
     gene            complement(11682..>12118)
                     /locus_tag="VFQ79_01835"
     CDS             complement(11682..>12118)
                     /locus_tag="VFQ79_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138414.1"
                     /translation="SAFIEAADIKPAESGGVKNDRVDPEGDAKRGFGNVPFHRKEFTA
                     KSVKAYFNLDLALLRSYRLSENALELLIALALFKIRRLVGSPIRLRTACDFELKNGLV
                     VTRPAGYQLPSQEELTKLVANGITGLKSSGELGEVIEVAWKE"
BASE COUNT         2420 a         3630 c         3644 g         2424 t
ORIGIN      
        1 gtcaggcatg gcggcatccg accagaattg gaatgagcac gtgtgcataa atcgtagccc
       61 cagacgtttc ttccgtcaag acccaaaatc gtaaatttgc taaaaatctg ctttacaagc
      121 aagctcagga tgaatagaat cgtaatgcac acgtgttcaa acggcacaga cggatgctga
      181 tacttccgtg aggaacgcga acaaaccggc ttccatcaaa gacatcgccc gcattgcgcg
      241 cgtatcgcat tccactgtat cgcgcgcatt gcgcgaaagc ccgttggtga acccggaaac
      301 cgctgaaaag atccgccgca tcgctgcgga aaacggattc agaaccagcg ccatcgcccg
      361 cagtcttgct actagccgga ccagtaccat tggcgttgtc gtaaccagca ttgccgatcc
      421 gttcgtggcc gaagtagtca gtggaatcga gagcgaagcg attgcacaca actactccgt
      481 gtttctcgcc aactgcaacg ccgacccggg gcgcgagctc aaggtcgtgc attcgttcga
      541 ggaccgccgc gtcgatggca tcgtagtaac cgcatcgcgt gtgggcgcgc tctatggttc
      601 gctgctcgag cagatgcaga tcccgatcgt gctgctgaac aaccagcatc ccagccactt
      661 cgcacactcc gtgctgatag agaacttcga ggcaagccgc ggcgccgtga acctgctcgt
      721 tgaactcggt catcggcgca tcgcctacat cggagaccgt ttcggataca gctcggattc
      781 ggaacgcttc agcggttacc ggtcggccct ggatgaagcg gatatcccct ttcaaccgga
      841 cctggtcgtt cacggcgacg gcaaggcgga aggaggaagc gaggccatgg agcggctgct
      901 ggccgcgccc gaacggccca cggccgtatt ctgctacaac gacatgaccg ctatcggcgg
      961 cctgaaggcg attcgggcgc acgggctcaa ggttcccgac gacatttcgc tggtaggctt
     1021 tgacgatctg ccgctggcgc tgtatgtcga ccctccgctt acgaccgtgc gccagccgaa
     1081 attcgatatg ggccgcctgg caatgcaggt tttattgaag ctgatcgccg gtgtggatgc
     1141 cgaacaggac gttaaggtga ccggcaagct catcgtgcgc cagtccaccg cgccccccaa
     1201 ggagaagacc gaatgcaact actcgtacgc ccccaaaacg ggacgcaagc ggaaacgcat
     1261 ggctttcagt tcctcgcgtt cagcaaccgc aaattgagac cgggcgagcg tgtggaagcg
     1321 gatacggcgg accgcgaact gggcctcgta ctgctgggcg gaacgtgctc ggttgcttcg
     1381 tctcgcggca agtggaccgg catcgggcgg cggccgcacg tgttcgcggg aatgccccat
     1441 gcgctgtacc tgccggttgc cacccgctat accgtaatgg ccgataccga atgcgacctc
     1501 gcattctgct attgcaaagc ggaagagtac catccgccgg cactgattac gccggccgaa
     1561 atcgaggtgg agattcgcgg agccggaaac gcgacacggc agattaacaa gatcatccgg
     1621 cccgagttcc ccgcgcaccg cctgctggtt gtcgaggtgt acacgcccgg tggaaactgg
     1681 tcgagctatc ctccgcacaa gcacgacgtc cacaatccac ccggagaggt tgacctggaa
     1741 gagatctact attaccgcgt cgaccgcacc gagggttatg ccatccagaa ggtctatacg
     1801 cccgaccgcc ggctggatga gaccatcacg gttcgcgacg gcgagatggt attagtcccc
     1861 gaaggctatc atccggtggt cgctgcacac ggttacaacg tttactattt gaatgccctg
     1921 gcaggctccg cacggtctat ggccgcctcg gacgatcccg actacatctg ggtccgggat
     1981 acctggcgtg acaaagaccc gcgcgtgccg ctggtcacga tggaaatgga aaactgagct
     2041 ttcgaggtct cgacggatgc gaacacaacg actaaccacg gcccaggcac tggtccgatt
     2101 tctgaagaac caatacgtag agcgcgacgg gcgggagcat ctgttttttc acggcgcctg
     2161 gggtattttc ggtcacggct gcattgcggg cctgggtcag gcgcttcagg agaatccgga
     2221 ttttcgttac taccagtcga agaacgaaca ggcgtcggta cacatcgcga ccgcgtttgc
     2281 gaaaaccaga aaccggctcg ggacgtttgc ctgcatctcc tcgatcggtc ctggcgcgac
     2341 gaatatgatc acgggcgccg cgacagccac catcaatcac atccccgtac tgctgctgcc
     2401 gggcgaccta ttcgcgcggc ggaatgtagc tcccgtattg cagcaactcg aatccgagca
     2461 tagccaggat gtctcggtga acgatgcatt caagcccgta tcgcgttatt gggaccgcat
     2521 caatcgtccg gaccaactga tctgttccgt ggtcgaagcg atgcgagtac tcacttcccc
     2581 ggcgcagacg ggcgcggtaa ccctcgcgct gccacaggat gttcagtccg aagcgtggga
     2641 ctatcccgag gagatgttcg agaagcgcgt gtggcatatc ccgcgcgcag aaccggaccg
     2701 cgcgatactg gcgcgggccg cggaaaggat ccggtcctca aagcggccgc tcgtcgtcgc
     2761 aggaggcggc gtgctataca gcgacgcgac ggaagccctg gcacaattcg cggcacaaac
     2821 cggcatcccc gtgggagaaa cacaggccgg gaaaggatcg ctgccgtttg atcatccgca
     2881 gaatttgggc gccataggcg ttacgggaac gcccggcgcc aacatcgcag cgcgcgatgc
     2941 cgacctggtg atcgccgttg gcacgcggct cagcgacttc accaccgcgt ccaagacagc
     3001 cttccagaat ccggatgtgc aattcatcaa catcaacgtt gcggagtttg acgcgttcaa
     3061 acacgccggt ctccccctgg ttgccgacgc tcgcgtcact ctcgaagcac ttggcgccgc
     3121 actggccgga taccgtgtgc aggacgattt tgcgaattgc atcccgcaat ggaaagaccg
     3181 ctgggaaaaa gagaccgacc ggatttacgg cttgcggcac ggcccgccca tcagccaagg
     3241 cgaggtcata ggggcggtaa accgcgtgtc gcggccccag gacatcgtcg tttgtgcggc
     3301 gggcagcctg cctggcgatc tgcataagct ctggcggacg cgccagccga atggttatca
     3361 cctcgagtac ggctactcgt gcatgggata cgaaatcgcc ggcggactcg gggtgaagat
     3421 ggcggacccg tcccgggaag tctacgttat ggtcggcgac ggctcgtttc tcatgatgtc
     3481 gtccgagatc gccacctccg ttcaggaagg ctacaagctc aatatcgtcg tgctcgataa
     3541 ccacggtttc tcgagcatcg gcgggctgtc tcaggcgatc ggatcgcacg gcttcggtac
     3601 ggattaccgg cacagaacgg aatccggcca gctcgatgga gagcacgtgc ccgtcgactt
     3661 tgccgcgctg tgccagggac tcggtgcgcg taccgttcgg gcccgcacgc gcgaggaact
     3721 ggagaacgca ctcgaagcaa tgaagcgcga cgaccgcacc acggcggtgg tgatcgaagt
     3781 ggataaggag atgcgcgttc ccggttacga gtcctggtgg gatgtgccca tctcggaggt
     3841 ttccaggatc gagagcatcc gcgccgcccg cgccgcctac gaagaagcac tgaaaaggga
     3901 gcggcgtcac gaagtggccg ctggtgcaag atagaaccat gactggaaca aatgaacttc
     3961 gcacggacgt cctggattac attggaggga cttggcgccg gccttcctcg gatgcggtga
     4021 cggaggtact gaatccggct acaggcgatg tgatcgcccg cgcacccgca ggctccgcac
     4081 aggatgtcga ggccgccgtg gaggccgcat cggctgcgtt tccggaatgg cgctcgactc
     4141 cacccgccga cagaattcag tacctgtttc gtcttaagca gcttcttgac gatcacttcg
     4201 aggagatcgc ccggattact acaaccgaaa acggcaagac tctgtccgaa gctcgcgggg
     4261 agctgcggcg cggcatagag aacgtggagg tggcgtgcgg catccccatc ctgatgcagg
     4321 gctacaacct ggaggacgtc gcaagcggca tcgatgagtt gatgttccgc cagccgctgg
     4381 gcgtcgttgc cgcgatcacg cccttcaatt ttccgtcgat gatcccgctg tggtttctgc
     4441 cgtacgcaat cgcctgcggg aacacgttcg tgctgaagcc gtccgagcgc gtgcccttca
     4501 gcatgatccg tatcgccgag ctgatcgagc agacaggcct tcccaaggga gttgtgaaca
     4561 tcgtcaacgg ggggaagaat gcggtagacg ccctgctcga tcatccctcc gtgcaggcgg
     4621 taagcttcgt gggatcgacg gcggtggctc gctacattta ctcgcgggcg tccgccgccg
     4681 gcaaacgtgt gcagtgccag ggaggagcga agaatcacgt cgtcgttctt ccggacgccg
     4741 accgggaaac gaccacccgg atcatcgccg acagcgcata cggctgcgcc gggcagcggt
     4801 gtttagccgt ttccgtggcg gtgactgtcg gagaggcggg tgaatggttt ccgaagtcca
     4861 ttacggaaac agccgccgct ctgaaggtgg gctccggact cgaagacggc gtgcagatgg
     4921 gtcccgtcat tacgcccgcc agccgtgagc gcatccgaag tctgatcgca cagggcgcct
     4981 ccgacggcgc gcggcccatt ctcgacggcc gtggcataag ccttagcgaa tggcctcgcg
     5041 gcaacttcgt tggcccgacg gtactcgacg gtgtgccggc ggcaagcagt ctggcggaaa
     5101 cggagatctt cgggccggtc ctcagcgtca ttcatgcttc gggtgtggac gaagcgatgg
     5161 aaatcattgc gcggaactct tacggcaacg cctcatcgat cttcaccacc agcggtgccg
     5221 cggcgcgcaa attccgcaat cagattccca cgggcaacgt gggtgtgaat atcggagtgg
     5281 ccgcgccgat ggcgtatttt ccgttcagtg gctggaaggg cagcttcttc ggggtccttc
     5341 acgcacaggg acgcgacgcc gtcgagttct ataccgacaa gaaggtcgtc atcgaacgct
     5401 ggccgaaaga gtggagccgg aggttctaat gtcaacggca gtcgggagca acgtggcaat
     5461 gcttcgcgtc gggaatgcac cctgttcgtg ggggactctc gagttcgaag aagccaaagg
     5521 cgaacagatc gcgttcgatc gaatgttgga cgaactcgcc gagaccggtt acaccggtac
     5581 ggaattgggc gactggggct acatgcccac ggatgccgcc gttctgaagg ccgaacttga
     5641 caagcgcggc ttggtgatgc tgggcgcttt tgtgccggtt gccctgaaag acccggcagc
     5701 ccacgagccc ggaatcgcga atgcactgaa gaccgcccgc cttctggcgg cggtctcggc
     5761 agagccgaag ccgtatctcg ttctggccga caacaacggc acggttccgg agcggacccg
     5821 caacgccgga cgcatcacgc cggaaatggg tctgaacgcg gaggagtggc gcaccttcgc
     5881 cggcggcgcg aatcgtctcg gccgcgtggt gctggaagaa accggcctgc ggaccgtgtt
     5941 ccatcatcat tgtgccggct acgtggagac acccgatgag atcgcacgct ttctcgaact
     6001 caccgatccc ggtgcgatag ggctggtatt cgataccggc cattatgcct acggtgcagg
     6061 gcactgcgat gtcctccagg gtttggagcg atacaaggaa cgcgtcctct acatgcacct
     6121 gaaggactgc cagcccgaag tggcacggca ggcgcggatc aacgagtggg actatttcga
     6181 agcgctgcgg catggcgtct tctgcgagct tgggcagggg ggtgtggact ttccggcggt
     6241 gttgcagtgg ttaaagaaga attcgtatgc gggctacacg ttggttgagc aggacgtgct
     6301 gccgggaata ggcgcaccca aagagagcgc gcggcgcaac cgcgagtatc ttcggtcgat
     6361 tgagagggcc ttgtgaataa acaactgaac ttcggtctga tcggcgccgg ccgcatcgga
     6421 aaagtgcacg ccgagacgat cgcatttcgc atccccgaag cggctctgac cgcggtttcc
     6481 gatatcgacg ccgaagcggc ggatgcggtt gcccggtgtt gtggaacccc gcgcgtcgcc
     6541 gccagcgccg ccgaaatctt tgccgatccc gctatcgacg cggtgatgat ctgctcttcc
     6601 accaacaccc atgccgattt gatcatcgaa gccgcgcagg ccggcaagca tatcttttgc
     6661 gagaagccga tcgatcacag cctcgaaaag atcgaccgcg cgttggatgc cgtgaacagg
     6721 gcgggcgtca agttgcaaat cggattcaac cgccgtttcg atccgaactt cgcgcgcgtg
     6781 cgcaggtcta tcgccacggg agaaatcggc acaccgcacc tgctgcatat cgtcagccgt
     6841 gaccccggcc caccgccgct cgcttacata aaagtatccg gcggcctgtt tctcgacatg
     6901 acgattcacg atttcgacat ggcgcgtttc ctcgtcggca gcgaagtgac ggaggtgtac
     6961 gcggccgccg gagtaagagt tgatcccgat atcggaaaag cgggcgatgt tgatacagcg
     7021 gtaatcgtgc tgcgcttcgc gaatggcgtc atcgggacca tcgacaactg ccggcaggca
     7081 ccctatggct acgatcagcg ggtggaagtg ttgggcggcc tgggcgcgat cgcgacggag
     7141 aactgctatc cgaaccgcgc ggtcatcagc acggcggaat ccgtgcggcg ggatttgccc
     7201 ctgaattttt tcatggagcg ctacgcggaa agcttcgcca acgagttaca ggcgtttgtc
     7261 gaagcgatcg tccacgacca gcagacaccg gtgacgggcc ttgatggccg tgtacccgtg
     7321 gtaatggcgc tggcggcccg gaagtcgcac gaagaaggcc gtccggtgct gctcgatgaa
     7381 attgccgcgc cggaggcccg ccttgaaacc atctctcatt agagccacga tcacggatat
     7441 ccagttctgg ctgccggtgg tcgttctgat attcggaatt gctctcctcg tggtgctctc
     7501 atgaccttac cggcaagagc cgcaaagcaa tccactcatt ccttgcatac gctcggcgtc
     7561 gcttctggac tggcggccgg cgcgtggctt ggaatggcgg aagcaccgac gaaactggtg
     7621 accgtgggct actcgccatt cctgatctcg ctgggaatgg tggccggcgt cttcgtcgcg
     7681 cgctggacgg ttcctgttgc gctgaaggga accacttaca tctgggcgga ccttcgcgaa
     7741 aaaccgcacc tcattgtttg ggcggtgctc gccggaatgc tgtgggccgt tgcgaacacg
     7801 ctcaccattt tcgccattcg caatgtcggg ctttccatcg cgtttccgct gtggaatacc
     7861 aacagccttg taggcctgtt ctggggatgg ttgttattta acgaactgcg gggcgcgggt
     7921 ggcagacaac gcgcgaaggt gctaggcgga gcggcggcca tcgtgaccgg cgcctgtttg
     7981 ctggcgtatg ccacatcgca ccaatcctcc tccggtcccg ccggtgccgc caccttggga
     8041 gtgatagcgg cgattggcgc gggcgttctg tggggaacga tgtacattcc ctaccggaag
     8101 gcgtacatca gcggcatgaa cccgctgtcg ttcgtgaccg ttttcacgtt cggcgaattg
     8161 gggacggtct tcacccttgc cgtcgtattc gacggtggag ttcagaacgt catcgttgct
     8221 ctgggacacg cgcggcccgc gctgttctgg ctgtttctcg gcggtttctg ctgggttctg
     8281 ggcgatctgt ttcagcagta cgccgcgaag tacatcggta taagccgcgg aattccgctg
     8341 tccaacacaa atcaactttg gggtctcgcc tggggcgcgc tcgtatttgg cgagttcgcg
     8401 gcccacggct tcgccgcgca actgcttatc gtcgccggat ccctcgtcat gatcgcaggc
     8461 gcggcggcca tcagtacggc agaggcgccc gagtccgaac agcggtcctg gcgggacgcg
     8521 atggaacggg agtgcgatcg ttacggcctc gatcgcgatc gtgtcgcttc caccatgcag
     8581 ggcgaagatc cgctggccgc ggaacgccgg ccgcgccgct ggtgggagtt agtgattgta
     8641 gcgggagccg tggcgttgtt cgtctggctc gctgccggcg cggagcgtca acccctggat
     8701 gtcagcattc catggatggc cgcgctggtt gtcgcctcgc tcgtattcct gatcgcctgc
     8761 gggctgttgc tctggaagag aacccgcttt tcctgaggcg atttgtgggg cgggaattca
     8821 gcccgtctgt ttgccgaagg ctctgctggt ctctttccag cggccccggg tgaaatccgg
     8881 gaacggaacc ggcgcgctgt tcttcgcgac ggacagatcg ctcaacggtc cgggtgcgct
     8941 ccacgccgcg gcgtcgtaga catccatatc cggcacaatg ccttccttca tgcactgtat
     9001 gaggcgatag cactcgatga aatccatccc gccgtgtccg ccgagcttcc gggccgtctc
     9061 accaagctcc ttccaataag ggtgttcata ttgagccttg aaagagtcga tggatcccca
     9121 ttcgtgcttg cccatgctat ccagataaat gcgcggcggg tagtcgcgaa aaataccttt
     9181 gacgccggaa atcaggttga tcctgtcgta gggctgcgga gacgtgacgt tgtgttccaa
     9241 cgtaatgagc ctgcccttgg tggtcttgat gaggctgatg ttcatgtcgc cgcagcgata
     9301 agtttccttg cggcgcgggt cgtttgccgg aaccttctgg tcccggaaat aggctaacga
     9361 gatagccgga gaactcatcg acaccatgga ctcgaacttg tcgccccgat taatgtccat
     9421 gtaattggcc accggaccga gaccgtgcgt cggatacagg ttgccattgc gcttaatatg
     9481 atctgtccgg cgccacagac cttcgccgcg gtccgagaac aggaggtcgc gcaggtcgtg
     9541 attgtaggcg caagccccat gcgtgagatc cccgaagacg cccgcgcgga ccatgttcag
     9601 cacgagaagt tcgttatatc cgtagcagca gttctcgagc atgacacaat gccgccgggt
     9661 tttttccgac gtattcacca gctcccaaca ttcttcgata gtcgttgccg cgggcacttc
     9721 taccgccacg tgcttgccct gcttcatggc gtaggcggcc atgggaacgt gccagttcca
     9781 gggggttccg acgcaaacca ggtcgatgtc gtcgcgcttt gccaattcct cgaacgcccg
     9841 ctcgccctga tggtagtagg tgggaacctt ggagtgtccc aatttctcga ggatggcctg
     9901 cgcgcgggga agcctttcct tatcgatgtc gcacaaggcc tgaacttcga ccccatcgac
     9961 agccgcgaag ttccgcatga gccccgttcc gcgtccacct aaaccaatga atcccatccg
    10021 aacggacttc ttcggttcaa acttcatctc gtaaacggtg tcgccctggg ctttcggctg
    10081 ctggccctgt gagagccccg cgccgagggt ggttaaggag gcaaaccgca gcaaatcgcg
    10141 gcgggatgat ttcgtcagat cgtctttcat atgtaattcc tatcgaaggt taacttagat
    10201 tatggcgcgt tcgtaaggct cgcgggtgga tggaatacgc tttactccaa agcccggaac
    10261 tgccctaaac cgaaatggca cccatagccc agcgaaatcg gacctgcaac gggttcggta
    10321 aactggacaa caaatccgtt gccatacaat tgcccatgtg ttccgccgcc gagcaaccgc
    10381 tctaatcgat actcgaccca ccgccgtccg cgcatatccc tttcatactg cgcgatcaca
    10441 cgcggcatag tgccgatgcc gcgtttctcc aattcgcggc aaagctgatc cgcagccgta
    10501 tcaacccaaa tcccaccacg ccgcttcgta tggcgtaccg gaacataggg tgtgatggat
    10561 tcccatttgg tcgatttgcg gaaaagatga tggtttccta catcggtttc ggttccgagc
    10621 gcgatcagaa cgggccggat atcggggcga ttctttccga cttgtcggaa ctcgcgaaaa
    10681 cgatccagcg ctcgcagttc tccatcgcca aaacccacct ccgcatagac tgttaaatga
    10741 tctatccgcc catcccggtc ttcatcggtt ggcagaaaga acgcgtgatt atggtcctgc
    10801 cgtggattgc cgtccgaatc cctgccgcta aagaccggtg atgcagtccc ggaatgcaga
    10861 agcccgtaac agccttgtag ttcctggcag gcgaactcgc caatggcgat cgtctctgta
    10921 actaatggcc ggacccgcga gtacagggcg aatcgtgcaa cagtgtactt ttttgacggc
    10981 ttctcgattc gccgcgatgc tccaccggca aaacaatcca accgccgcca atacgtcacc
    11041 caagccgatc ctggagggtc actccaccgt tcgtttcgta ggtccaatgt agtgcggcac
    11101 agcggccagg cttccggtgc cgtgccctgt gcgatggctc tcgccgaatc caccgtcaga
    11161 acccggaccg cttcagtatc cacatccgcg ccggccatac ccggtctcgg tggatgacag
    11221 ttcgtagctg ccgtagagcg ctccatcagt ctcgcttcgc accaggattc agcccggccc
    11281 aaataggtca actgccgtag tgcggcatca agcgacaacg cctcctcttg ggtcaagtcg
    11341 acgtcgggcc agacaacctg aagttcggta tttctatcca gcactgcgaa cgtgtcaaag
    11401 accagggtcc tgttacccct gtagagtggc atgtagtgcc gcgcgtgccc gatggttgcg
    11461 ggaggcagat agaattcggg cggagttgcg agtttctgta cgacggattc aagaacagca
    11521 tcgcttccat cggttcgcac cttccatgct gcgacaatcg cccgcaggat tcgccaggga
    11581 aatggtggcc actcgaccga gccctcgttt acatgccttc cccaggctgt cgcatgataa
    11641 tgcccggagg gaaatttcaa ggcgattgct gtcggcatcg cctactcctt ccaagcgacc
    11701 tcaatcacct cacccagttc accactgctt tttagcccgg taatcccgtt cgccacgagc
    11761 ttcgtcaatt cttcctgcga aggcaactgg taacctgcgg gccgggtcac cactaaccca
    11821 ttcttcagtt caaagtcgca cgccgtccgc agccgtatcg gactgcccac caatcggcgt
    11881 attttgaaaa gtgccaaggc aataagcaat tccaaggcat tctcactcag gcgatagctt
    11941 ctgagcaggg caagatccag gttgaagtaa gctttgacgg atttagccgt gaactccttg
    12001 cggtggaacg gtacgttacc aaatccacgt ttggcatcac cttccggatc gacgcggtca
    12061 ttcttaacac ccccactttc tgccggcttt atatccgctg cttcgatgaa tgcagaca
//
LOCUS       DASTMK010000044        19605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_514620, whole genome shotgun sequence.
ACCESSION   DASTMK010000044 DASTMK010000000
VERSION     DASTMK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19605
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514620"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFQ79_01840"
     CDS             complement(<1..663)
                     /locus_tag="VFQ79_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138415.1"
                     /translation="MMRSDVFRQSDALRHLLDYLGSKSFSDPPEEVKEYTIGIEACGK
                     PPEYDPQKDASVRVQVGRLRQKLEDYYKSEGSADPVVLELPKGRFALLVRQRPPESKA
                     PVRRFRVFRNQNRVTVVLALLLAAALVWAGLLYQRVREYEGGLGASVQAATVREFIPL
                     WGSFLSHNASSVVVFGSPSFFASAKHNLFVRLYGLTDPDNPKSSPEFDTVDSRVGPLA
                     GPR"
     gene            897..4073
                     /locus_tag="VFQ79_01845"
     CDS             897..4073
                     /locus_tag="VFQ79_01845"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0138416.1"
                     /translation="MPGVLLLLAAAPLRAQTTASILGTVTDDTSAVVVGAAVRAVNTL
                     TNEVRNTKTDETGYYRFAELGVGLYNVEAEAAGFKTAIRQGIELSLNRNARVDIQLSV
                     GAVTESVSVIADAPLVEATTNEMGVLVSQQRVVELPLNGRNTLGLVGLIPGAQGLQTG
                     NAQGFIENKVSINGAREEDSNWMLDGGDNTSTLRNYGNDVPNPDAVQEFRVVTSNYSA
                     EYGRTVGAVVNVVTKGGTNEYHGSLFHFLRNRSLNARDFFEPDTSPLVQNQFGGSMGG
                     RILRDKTFFFGSFQGFRQRTSDFRNNALVPTQAERSGDFSRTVIGGNPVIVRDPLTSQ
                     PFPNNIIPKDRLSPIAQKYLDIAVPLPNDPARGPNALVQRASHPRDNDQFLVKIDHLL
                     SNNHKLSGAYFWSDSVDTDRFLGSTAFAWRDIKSRQHNLNIHEYWTISPTMLNHFRAT
                     YTRSAGDRKIMPDNITMLDLGAKWTPPPDGPPMPPDFEVVGWFDNGTANGGPKYANHY
                     TLADTFDWVKGRHELKFGAEGWLQRMTDVSTAPRMGGSFVFDGSVTGNAMTDLLLSQV
                     RTLEVASQTYKSQNSWVLHWFVQDKLRVSRKLILNLGLRHELNTWPVHPLDALIAIVP
                     GRQSTCVPQAPVGAVFPCDDGVPRSGVLGDYNNFAPRVGIAYDLTGDGKTVVRTGYGI
                     SYSFNFFNALQEQQVSVPFQYRATIRNTTLEDPYAPIGGSPFPFLFDAAHLTFPASGS
                     YGFQDFNMRTGYVQQYNFSIQRQLGADWSVEAAYVGNVGRKLMGQTDFNAPLRTPDAN
                     RNNVDQRRPLYPAFRDMRMTGGFVNSSYNALQTRVERRFSRGLTVLGSYTFGKALDES
                     SWYSSRNEWADTYNRALNKGRGENDRAHLLSLSWVWEIPGFSGSSGLAKALFQGWSAN
                     GIATFYSGGPVEIATGKDNDFDGNSSGDRPDVVGAWKLDPNRSRSEVIQAWFNPKAFV
                     ENGLGQLGMLGRNVVTGPGMKNVDLGISRSFRVTEASRLQFRAEAFNLFNWVNLESPQ
                     GNVSNALFGQITSARDPRIVQFGLKFLF"
     gene            complement(4076..5176)
                     /locus_tag="VFQ79_01850"
     CDS             complement(4076..5176)
                     /locus_tag="VFQ79_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU0138417.1"
                     /translation="MCLSGSLDPAWVQSTAARAADRLLSHPWKVWFWGDSIGIEGLLD
                     AAELTGNPSYFRFVQGLFAGWLAREAARSAFDYTLPGVALLRVYQQTGELRLLDAALR
                     HAEYMQGFRQTEHGAYVLYENAAIELPPELPRDHPDYRSQTSKVANGGACVFVDSVHF
                     DGPFFAKAYQVTGEDRFRRLALDNILPQIELLFDEEEDLFHHFWMENTGRRNGVLWGR
                     GNGWGLLGLTSTLQFLPPSDADAGRLIEVLQRLARRLRSLQTPDGGWHTILDDPESYI
                     ETSIAAFVVDGFATAIGRGWLDRDGYLPVVHSALALLRQHVQADGRLAGVSYETFPST
                     RRQHYREMPRDAVVPWGQGPLLTALRACTRYD"
     gene            complement(5160..6299)
                     /locus_tag="VFQ79_01855"
     CDS             complement(5160..6299)
                     /locus_tag="VFQ79_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0138418.1"
                     /translation="MKITGVKTARLGGSSACLRIDTDEGIRGFGEATVETVPEAVMAV
                     VHRIAGEYLVGQDPRPVELHRRRMQDALWYHADAVVSSALTGIDHALWDIKGQALGVP
                     VYELLGGPVRDRVRVYKWIGESSRDNLQEQARKRVAEGLTAIKFSPTLDCPSPYPRCV
                     DEARQLVCQVREAVGPHVDLMLDPASRWKLAEARHVLSELEEFQILFAEDFISPYCGV
                     ETIAKLAASTRIPYALGDRLIGYRQFEEVIRHDAAAVLQPDIGHAGGLLEIRAIALAA
                     EQRGIRIAPHNALGPVATAAAIHLDLAIPNFLIQEIAGTEFFGAWNLGSYVDTEILTV
                     RDGYIDAPRKPGLGVTIDDAVFNGELPVREPPFFSDPKDFHVPEW"
     gene            complement(6316..7086)
                     /locus_tag="VFQ79_01860"
     CDS             complement(6316..7086)
                     /locus_tag="VFQ79_01860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0138419.1"
                     /translation="MITLDLSGRCVLITGALGAIADAMIRRFQEAEATLVLTDIHPDV
                     EGRRMCEERGFSDCYYQQMDVTDAAAVDRAVEDLFSRFPGTDIALGHAGGCGIHPFAS
                     TSAEEFERIVRFNYFGQVNFARAVLAQWIRCGTKGHLVFTSSLVGSLPWPNFSAYNSA
                     KAALEMTAKCLALEYAGHGVRVNVVAPGHVSTGASIKIYETEPAYRAIVDRVIPMRRM
                     VRPEAVADAFLWLCSPLAEDVNGQVIKVDLGESIPCIG"
     gene            complement(7083..7988)
                     /locus_tag="VFQ79_01865"
     CDS             complement(7083..7988)
                     /locus_tag="VFQ79_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0138420.1"
                     /translation="MTDGVIRLADAMGPRVVQFYVLEDDFGCVLFDAALPGSVQSRLA
                     RGEIPGPLTRVIISHADADHLGDAGSLREAEILCHALDRDWISDHDRLVTERYGPAYP
                     PESLRALRDACGPDFPVSRTLEDRELLRIGHRTWRVLHVPGHTRGHIALWREDDGVLL
                     IGDAVLGLAVPNRDGKPSIPPTHCHISEYLATIERLSDIPVSTVFSGHWPPLDASEFR
                     NLLADSKTCVLRDLECVASCSGEPLGVDGYVEQICSRFRSWPPVEDIHYRFAVAGYLE
                     YLTVKSVLQQLPGGRYTAARGVAHV"
     gene            complement(7985..8665)
                     /locus_tag="VFQ79_01870"
     CDS             complement(7985..8665)
                     /locus_tag="VFQ79_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0138421.1"
                     /translation="MAVSLKPLETTNLRSQIASNLREAIYRGDFRPGESIQEAMVASQ
                     LRVSRAPVREAILLLEKEGLVTVQANRGAVIRSLTQAELAEIMSMRYRLESWALELAR
                     PKVTPEIMAELRELHARMLEAAGRGDVRAFTNTDFAFHKLVWETGDNALLAETLQRIA
                     GPVFAFADIVLSQAGFDCSRLAELHHPIIAYLAGETEARPEDVLRDVFEYTFQSEWDV
                     LFRDKERR"
     gene            8807..12403
                     /locus_tag="VFQ79_01875"
     CDS             8807..12403
                     /locus_tag="VFQ79_01875"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138422.1"
                     /translation="MKHYTLVAMSVLCLCTMAVSVRGQDYRAKLSGEVTDPSGASMPR
                     VKVELTNKITLAVTSTETNESGLYQFQFLEPGEYRISVSSSGFKTFVQDGIRLQTGQR
                     AAIDIRLQVGDTAERIEVTAEVAALDTESASRGLVANTLLVQELPIRSRNPLNVVNLL
                     PGTTQRGPGVFMASFHNSANVNFAISGGSPQQNEILVDGAPNTVRTTGVQNNVALMPV
                     SESVGEVAIVTNAYDASYGRTSGGVVNFTTMGGTNEHHLTGWGFLRRKGWNANAYPLN
                     AIGSPRPEQQVNQYGFQVAGPVEIPKLIRKNGRTSLFYLASYERYGELWPQPIRVSVP
                     EPEMRTGDFSKLRNAAGDLIQIFDPFTATPDASGNPVRQAFPNNVIPASRIHPVAAAV
                     TKYYHDPNDRGLPNQRYSQGNFSLPLFSYDLNFWNWNSRVDANLGEMNRVFFRYSTNK
                     HTQERTINGIIGRPGEQAYNPFLRRNHALMLDWVRIINPSTTLNLRGNYARYIEGFDS
                     AGNFGFDLTTLGLPSSLVRQLAVPDFFGVWAIGGYTQLGFVPNLEYNNTYSLQSNITK
                     IWRSHTIKAGIDVRRWHFLVNNPVQPFRVLSSAAFTRQVWNNSASEVNSGDGFASFLL
                     GAVGGGSADFAVRPFYRSWYFAPFIQDDWKVTRKLTLNLGLRWDYNPTSDEKYNRLVT
                     GFDRTAVSPLASTYPQLGNLQGGLTFAGVNGERRQASNPDLNTIQPRIGAAYRIGEAT
                     VLRGGYGLFYANWPTGDYLQSQGFSTSTALVSSLDGSRTPRSEALTDPFPGGVQQPIG
                     AGLGLNTFLGSNFNWWNQDARLPRVHQFSFGVQRRLTSSSSIDVSYVGSRTHHFHTSL
                     PANLVSDDFIAQCDPSRGGNRSYCDALVPNPFVGLPEFAGTSLGLSSTISRLRASRPF
                     PQFDGDLVELGRSDGRMWYNSAQIVYRHVFQKGLIVNANYTFSKQVSQEGWLNNYAAI
                     PQRSLVSFDRTHVLKVAAHYELPFGKGRRFGAGAAPVLERVIGGWDLNVFYTASSGEP
                     ADLPGNAIMLKDPKIEADRGATIVRGWNPCVLQTNADGSIVPTRASTVVNGCSPTDFS
                     GYAWLVPRNEMLTYRTNPLRSGQIRMPAQYFADISLNKTVNITERLRFQFRAEAFNAF
                     NRFNVFSVRYNSNPLDANGNFGSYLPSDAGASSGPMRDCPPRSIQLGMKLMW"
     gene            12410..14317
                     /locus_tag="VFQ79_01880"
     CDS             12410..14317
                     /locus_tag="VFQ79_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138423.1"
                     /translation="MLSTRRTILLGGLSQLAWPAEDYTLRAGRAEARLAPSGGLADLR
                     FERTPLVSSGLGDGCPEISVDGKPIRCDRPVKASRTRDSIAFEYRIANLEVTYEVALR
                     ELKEDAAMLVQRVAWKSDQRSSGAIGLHVPRNVRLPAASRSMFVPTREGVGRRMAVPD
                     SRQPPWLYLMAGGDPEATKQRLAAPVLSEYSTADTLRLTIASEPGFTTSFRVAGASEP
                     GSFDWVHTTGGEAGQVTARTFYTVIHRGADRRAYYALYDSALANVRPGPAWLHDITMV
                     SYDYLSKNGRGWFADIDALAKLMPVSERNRVVLTIHGWYDYVGRYIFNPRSKTLDRTW
                     TAFSNVKSPQFLAHARGPRWKTATHWGWRKILDGLRPVEMSIADLRRRMRYARERGFR
                     CILYFGDGVNSGDGLADVHEPEKVLAWGGWIGPETSGKTYAQNPLHPGVQSFFKDYLA
                     ALLNEFGRDLDGFVWDETFHVRAGQKGTDAVPGYADAAMMNLVSDLRAATERHNPELA
                     FLTSDIVGPNYQAPYALVSHGTYQDSMSGLRYWPYGLFPNLRNSLWSCNWYHQTTWDR
                     NEKTVATYELPVAISNGYGDDTGPSDMNDEQIRKTMALFRRLGNRKLELEWIEESGAR
                     RTYKGRPVPTE"
     gene            complement(14349..14498)
                     /locus_tag="VFQ79_01885"
     CDS             complement(14349..14498)
                     /locus_tag="VFQ79_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138424.1"
                     /translation="MTHSLANRVKNEERLVKNVVENSLLQSGLLLASLIALVLIAAGV
                     VIYSR"
     gene            14635..16101
                     /locus_tag="VFQ79_01890"
     CDS             14635..16101
                     /locus_tag="VFQ79_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU0138425.1"
                     /translation="MRKTFLQPERDERDTVPPLRTAAEHAFSRAAGAPLIGGNHVRLL
                     KDARENYPAWLDAIAAAKHHVHFESYIIHEDEQGREFADALIAKAAEGVRVRLIYDWL
                     GGFGKTSKRFWNRLRAGGVEVRCYNPPRFESPLGWLSRDHRKMLVVDGETGFITGLCV
                     GQMWVGDQRENRDPWRDTGVEVRGCAVADIERAFAQIWALTGEPLPEDEASGLETPAP
                     AGGMMMRIVASVPATAGMLRLDQLVSALARNRLWLTDAYYAGTTLFVQALRAAAKDGV
                     DVRLLVPGGTDIPLLRPLSRAGYRPLLESGVRVFEWNGTMLHAKTAVVDGHWARVGST
                     NLNVASWLSNCELDAVIDDESFGREMEEMYLEDLSNSTEIVLNVKQKMRAPGAPRHPH
                     LGQGSGSVGRVAAGGIRLGSAIGAAFTDRRVLGPVEAQLMLMAGLLLISLAVLFAFFP
                     KVLVYPVIVFMTWMALALVYKAYRLRRNRKRQEQRPNL"
     gene            complement(16150..>19605)
                     /locus_tag="VFQ79_01895"
     CDS             complement(16150..>19605)
                     /locus_tag="VFQ79_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138426.1"
                     /translation="RYSIMIEAAGMENFKGEFLLQTGETAVVDATMTVGSTATEVTVI
                     ADAAPLVTTTSPTLAAVTDRARIDQLPISGRMFQSLVGRTTPGIDGASMTPRVWGLRW
                     GVEFLQDGAVLANRDIGEIAGRPPGMDTIEEFRVETNNSSAKMNRPGTVIVNTRAGTN
                     QFHGSLFEIVRNNNLGFGVARARQDKWSRPPHLVRNEFGASAGGPIYLPKVYDGRNRT
                     FFFSAYEAFRSRTATTKLARLPTAEMRQGDFSGLVDGSGRRYDLYDPWTSDANWSRQP
                     FPNNQIPMSRQSPLAKYLYSVTPMPTEPGVNPLLADNYFYQAPNNRMEWTTTTRIDHR
                     ISDADQIFFRYSHGVRDTYAQSGPNNSPTTLDFAANGTFRPIRNDTGVVSWTHTFSPT
                     FFSETSFNVAVEDLNLINVGDTEKWGDKLGLPNPFDEYGFPNITSTGVGMEYITAANR
                     RNSIIHIYNFDQNFTKVHGRHELMFGGRYRYEKMNVLPDQQQVQGAHAASSGATGLYD
                     PASGSTYAAVPYTGHNAANLFIGVMGSYSAQFVRKWYDLHDSEISLYLQDNFKVNSRL
                     TLNLGLRWEMYTPIREADNILTGFDPATKSVVNGADWETMYRANATTPAITKVYTDMG
                     LQFVRPQDVGLPQGLIHLNKWDFGPRAGFAYRFSDSAKSLVLRGGYGIYAYPMPLRDF
                     NARMRQNPPTTARFTNSLGDSSQTPDRLPAYGLRAVPQYVAGVNSRDVLDLNRPGGVS
                     RGSFLTSYFNPEQPSSRAHQWNLTFEKEVIDNTLLRVGYAATHGSRLDMYYSYNQAPN
                     SYVWFATQGVPLPTGTYAGTARRSFETTMFGEIEEYQKTGWSNSQNIQIEVQRRYSKG
                     YGFQFFYVLSNSLKAGGNGWGDDILPPASIYLPGAVPQDNNERARFLFYRRDTDIPKH
                     RFNWNWIADLPFGKGKRFASSAGPVLDRIVGGWQIAGDGSFTSTWWALPATNWSFPND
                     LEIYGTKYPIQDCRSGVCRDGYLYYNGYIPANRINSYDAQGRPNGVMGVPSNYKPAHS
                     PLIPTPADGGDRNDPMFAYYESNNVWVPMKDGSLQRIPLDTGLNPWRNQYGQGLFNWY
                     QNASLFKVIPVNERVFFRLTIDFFNVFNMPGIPKTPDSTSGIIDATTSGNGARSLQFG
                     LRLTW"
BASE COUNT         3919 a         5858 c         6020 g         3808 t
ORIGIN      
        1 cctcggaccg gcaagtggcc ccacgcgcga atccacggtg tcaaactccg gactcgattt
       61 cgggttgtcg gggtccgtca gcccgtagag gcgtacgaac agattgtgct tcgcgctggc
      121 aaaaaacgac gggctgccga agaccacgac cgaagacgca ttgtgcgaaa gaaaggaccc
      181 ccataacggg atgaattcac ggacggtagc cgcctgaacg gaagcgccca agccgccttc
      241 gtattcacga actcgttggt acagcaatcc cgcccacacc aaggccgcgg caagcagcaa
      301 tgccagcacc acagttacac ggttctggtt ccggaaaacc cgaaaccgcc ggaccggcgc
      361 tttggactcc ggcggccgct ggcggaccaa cagagcgaac cggcctttcg ggagttccag
      421 gacgaccggg tcggcgctgc cttccgattt gtagtagtcc tccagttttt gccggagccg
      481 gccgacttgc accctgacgg aagcgtcctt ttgcgggtcg tattcgggag gcttgccaca
      541 agcctcgatt ccgatcgtat attcttttac ttcctcggga ggatcggaaa acgacttcga
      601 gcccaaataa tcgagcagat gccggagcgc gtcggactgg cggaacacgt cactccgcat
      661 cacacggtga agttgctggc gcgcagctat caaggcttca ctttcgaccg ccaagtgaca
      721 ttgtaccgcc ctgctcgtga tgtccaggta acttctccgc tacagttacg agaccgtaac
      781 aggctggtgt atcaaagcgg tgggagcaca taattggggg cgaaccatca acatcatcga
      841 agatatgctt caggcgaggt gagtttatga agtgtctaag ttccttctgg acagcagtgc
      901 caggtgtcct cctgttactc gcggcggcgc cccttcgcgc tcagaccacg gcctctattc
      961 tcggaacggt caccgacgat acgagcgccg ttgtcgtagg agccgcagta cgggccgtga
     1021 acacccttac caacgaagtc cgcaacacca agaccgacga aaccggctac taccgttttg
     1081 cggagctggg cgttggcctg tacaacgtgg aagcggaagc ggcaggcttc aagacggcta
     1141 tccggcaggg catcgagctg tcattgaatc gaaatgcaag agtcgatatc cagctttccg
     1201 tcggagcggt tacggaaagc gtcagcgtga tcgcggacgc accactagtg gaggcgacca
     1261 cgaacgaaat gggtgttctg gtcagccagc agcgtgtagt ggaactgcct ctgaacggca
     1321 ggaatactct cggcctggtg ggtctcatcc cgggcgcgca gggactccag accgggaacg
     1381 cgcagggatt tattgagaat aaggtctcaa ttaacggcgc ccgcgaggaa gacagcaact
     1441 ggatgctcga cggcggcgac aacacctcca cgctgcgaaa ctacggcaac gatgttccga
     1501 accccgatgc cgtgcaggag ttccgggtgg tgaccagcaa ctactcggcg gaatacggca
     1561 ggaccgtcgg cgccgttgtg aacgtcgtca caaagggagg tacgaacgaa tatcacggca
     1621 gtctcttcca tttcctgcga aatcgcagcc tgaacgcccg cgacttcttc gaacccgata
     1681 cttccccgtt ggtgcagaac cagttcggag gcagcatggg aggccggatc cttcgcgaca
     1741 aaaccttctt tttcggtagc tttcagggat tccggcagag gacatcggac tttcgcaaca
     1801 atgccctggt tccgacgcag gccgaacggt caggcgattt ctcgaggacg gtcatcggag
     1861 gaaacccggt tatcgtgagg gatccgctaa cgtcgcagcc ttttcccaat aacatcatcc
     1921 cgaaggaccg gctcagcccg atcgcgcaga agtacctgga cattgcggtt ccgctgccga
     1981 acgatcccgc cagagggccg aatgccctcg tgcagcgtgc gagccatccg agagacaacg
     2041 accagtttct cgtgaaaatc gaccaccttc tttcaaacaa ccacaagctt tcgggtgcct
     2101 atttctggag cgactccgtc gataccgacc gcttcctcgg cagcacagcc ttcgcctggc
     2161 gcgacatcaa aagccgccag cacaacctca acatacacga atactggacc atcagcccga
     2221 cgatgttgaa ccacttccgg gcgacgtaca cacgcagcgc cggcgatcgc aaaatcatgc
     2281 cggacaacat caccatgctc gatctggggg cgaaatggac ccctccgccc gatggccctc
     2341 cgatgcctcc cgatttcgaa gtcgtcggct ggttcgacaa tggaacggca aacggcggac
     2401 cgaagtacgc caatcattac acgctggcgg acactttcga ttgggtgaaa ggccggcacg
     2461 agttgaagtt tggcgcggag ggctggctcc agcgcatgac cgacgtttcc acggcgcccc
     2521 gcatgggcgg ctctttcgta ttcgatggaa gcgttacggg gaacgcgatg accgatctgc
     2581 tgttgagcca ggtccggacg ctggaggttg ccagccaaac ctacaaaagc cagaactcct
     2641 gggtactgca ctggttcgtg caggacaagc tgcgggtcag ccggaaactg atattgaatc
     2701 tcggtctccg gcatgaattg aacacctggc cggttcatcc cctggatgcg ctgatcgcga
     2761 tcgttcccgg caggcagtcc acgtgcgtgc cccaggcgcc agtgggcgcg gttttccctt
     2821 gcgatgacgg agtgccgcgc tcaggcgttc tcggggatta caacaacttc gctccccgcg
     2881 tcggcatcgc ctacgacctg acgggcgacg gcaagacggt ggtccgcacc ggatacggca
     2941 tctcctattc tttcaacttt ttcaacgcac tccaggagca gcaggtcagc gtgccgtttc
     3001 aatatcgggc cacgatacgg aacacgactc tggaggaccc ctatgctccc atcggcggtt
     3061 cgcccttccc gttccttttc gacgccgccc acctgacatt tccagcctcg gggtcctacg
     3121 gattccagga cttcaacatg cggaccggct atgtacagca gtacaacttc tccattcagc
     3181 ggcaactcgg ggccgactgg tctgttgagg cggcttatgt cggcaacgtg ggccggaagc
     3241 tcatgggcca gaccgatttc aacgcgcccc tccgtactcc ggacgccaac aggaataacg
     3301 tcgaccagcg gcggcccttg tatccggcct tcagggatat gcgcatgacg ggtggattcg
     3361 taaattcttc gtacaacgct ctccagacgc gggtggaacg gcgcttcagc cgcggactca
     3421 ccgtgctggg ctcctacacc ttcggcaaag ctctggatga aagcagttgg tacagttcgc
     3481 ggaatgaatg ggccgacaca tataaccgcg cgctgaataa aggacgcggg gaaaacgatc
     3541 gcgcacacct gttgtcattg tcttgggttt gggagatccc gggtttcagc ggttcatccg
     3601 gtctggccaa agccctgttc cagggatggt cggcgaacgg tattgccacc ttctattccg
     3661 gcggtcccgt cgagatcgcg accggcaaag acaacgattt cgacggcaat tcctcgggtg
     3721 accgtccgga cgtggttggt gcgtggaaac tcgatccgaa ccgctcccga agcgaagtga
     3781 ttcaggcgtg gtttaatccg aaggcattcg tggagaacgg gctgggacag ctcggaatgc
     3841 tggggaggaa cgtggtgacc ggtccaggga tgaagaacgt ggatctcgga atctcgagga
     3901 gcttccgggt aaccgaagcc agccgcttgc agttccgggc ggaggcattc aacctcttca
     3961 actgggtgaa tctcgaaagc ccgcagggca atgtctccaa tgccctgttc ggccagatca
     4021 ccagcgcgcg cgatccgcgc attgtgcagt tcggactgaa gttccttttc tgaaactagt
     4081 cgtacctggt gcaggcccgc aaggcagtca acagcgggcc ttgtccccac ggcaccacgg
     4141 catcacgagg catctcgcga tagtgctggc ggcgtgtgct cgggaaggtt tcataagaca
     4201 cccccgccag ccgtccatca gcctgaacat gttgccgcag taaagctaaa gccgaatgca
     4261 ccaccggcag gtaaccatcg cgatccagcc aaccgcggcc gatggcagtg gcgaagccgt
     4321 cgactacaaa cgcggcaatc gaggtctcaa tgtaggattc gggatcgtcc aatatggtgt
     4381 gccagccacc atccggagtt tgcagggagc ggaggcgccg cgccaaccgc tggagcactt
     4441 cgatgagccg cccggcatcg gcatcagacg gagggaggaa ctgtaacgtg gacgtcaggc
     4501 caagcagacc ccagccgttg ccccgtcccc agagaacgcc attgcgccgg cccgtgttct
     4561 ccatccagaa gtgatggaac aggtcctcct cttcgtcgaa cagaagttcg atctgcggca
     4621 gaatattgtc gagtgccagg cggcgaaacc ggtcctcccc ggtcacctgg tatgctttgg
     4681 cgaaaaacgg gccgtcgaag tgaaccgagt cgacgaagac gcacgccccg ccgttcgcta
     4741 ccttgctggt ttgcgagcga tagtccggat gatcccgcgg taactccggc ggcagctcga
     4801 tggcggcgtt ctcatagagc acatatgcgc cgtgttcggt ttgccggaag ccctgcatat
     4861 actcagcgtg ccgcagagcg gcatcgagca gcctgagttc accggtctgc tgatatacgc
     4921 gcaggagcgc cacgccgggc agtgtatagt caaacgcgct gcgtgccgcc tcccgcgcca
     4981 gccagcccgc gaacagtcct tgaacgaagc ggaaatagct tgggttcccg gtcagctccg
     5041 cggcatccag caatccttcg atgccgatcg aatcgcccca gaaccagacc ttccacggat
     5101 gtgagagcaa gcggtccgct gcgcgagctg ccgtggattg gacccaggcg ggatccagac
     5161 taccactcag gcacatggaa atccttcgga tcgctgaaga agggcggctc gcgaaccggt
     5221 aattcgccgt tgaacaccgc atcgtcgatg gtgacgccca ggcccggctt tcgaggtgcg
     5281 tcgatgtatc cgtcgcgaac ggtaagtatt tcggtatcga cgtagctgcc caggttccac
     5341 gcgccgaaga actccgtgcc ggcgatctcc tgaatcagga agttcggaat agccaggtcc
     5401 agatgaatgg cggccgccgt cgcaacgggg ccaagagcgt tgtgaggcgc gatacggatg
     5461 cccctctgct ccgcggcgag agcgatggcc ctgatttcga gcaggccccc ggcgtgcccg
     5521 atgtcgggct gaagcactgc ggcggcatcg tgccggatca cttcctcgaa ctggcggtag
     5581 ccgatcagcc ggtcgccgag tgcgtacgga atgcgggtgc tggcggccag cttcgcaatg
     5641 gtttccacac cacaatacgg cgaaatgaaa tcctcggcga acaggatttg gaactcttcg
     5701 agctccgaca gaacgtggcg ggcttcggcg agtttccacc ggctggccgg gtcgagcatg
     5761 agatcgacgt gtgggcctac ggcttcgcgc acctggcaga caagctgccg ggcttcgtcg
     5821 acgcatcgcg gataaggtga gggacagtcg agggttgggc tgaacttgat ggcggtcaag
     5881 ccttcggcca cgcgcttgcg cgcctgctcc tgcaagttgt cccggctgct ctcgccgatc
     5941 cacttgtaga cgcgcacgcg gtctcgcacc ggtcccccca gcagttcgta cacagggact
     6001 ccgagcgcct ggcctttgat gtcccagagc gcatggtcga tgccagtcag ggcgctcgac
     6061 acaaccgcgt ccgcgtggta ccagagcgcg tcctgcattc tgcgccggtg cagttccaca
     6121 ggacgcgggt cctggccgac gaggtattcc ccggcgatcc ggtgaacgac tgccattacc
     6181 gcttccggga cggtttcaac ggtcgcctca ccgaagccgc ggataccctc gtcggtatcg
     6241 atccgcaggc aggccgagct gccgccgagc cgcgccgtct tcacgccggt gatcttcatc
     6301 tcgggtttcc tttctttagc cgatgcatgg gatgctctcg cccaggtcaa ccttgatcac
     6361 ctggccgttc acgtcttcag ccagcggaga gcagagccag aggaaggcgt cggcgaccgc
     6421 ctccggacga accatcctgc gcatggggat cacacggtcc acgatggcgc ggtatgccgg
     6481 ctcggtctcg tagattttga tggaggcgcc cgtggaaacg tgcccaggag ccaccacgtt
     6541 gacccgcaca ccgtgtcctg cgtattcgag ggcgaggcac ttcgccgtca tctcgagcgc
     6601 cgccttcgcc gagttgtacg ccgagaagtt cggccaggga agcgagccaa ccagcgacga
     6661 ggtgaagacc aggtggcctt tcgtcccgca ccggatccat tgcgccagga cggcacgcgc
     6721 gaagtttacc tgtccgaaat agttgaaccg gacgatgcgc tcgaactcct ccgccgaggt
     6781 ggaagcaaac gggtggattc cgcagccgcc ggcgtgtccc aacgcgatgt ccgtgccggg
     6841 aaatcggctg aagaggtcct ctaccgcgcg atcaacggca gccgcatcgg tgacgtccat
     6901 ctgctggtaa tagcagtccg agaagccccg ttcctcgcac atccggcgtc cttccacgtc
     6961 gggatggatg tcggtgagga cgagggtcgc ttccgcttcc tggaagcggc ggatcattgc
     7021 atcggcgatg gctcccaggg cgccggtgat gaggacacat ctgccgctga gatcgagcgt
     7081 aatcatacgt gggcgactcc tctggcggcg gtgtaccggc ctccgggaag ctgctgtaga
     7141 accgacttga ccgtcaggta ttccaggtaa ccggccaccg cgaagcggta gtggatgtcc
     7201 tccaccggag gccacgaacg gaaccgggag cagatctgtt ccacatagcc atccacaccg
     7261 aggggttcgc cagagcacga agccacgcac tcgagatcgc gcagcacgca ggttttgctg
     7321 tcggccaaca ggttccggaa ctcgctggcg tcgagcggtg gccagtggcc ggagaagacc
     7381 gtcgatacgg gaatatcgga aagccgttcg atcgtagcga ggtattcaga gatgtggcaa
     7441 tgcgtgggtg ggatggacgg cttgccgtcc cgattgggaa cggcaaggcc gagcaccgca
     7501 tcgccgatca gcagcacacc atcgtcttcg cgccagagcg cgatgtgccc ccgtgtgtgt
     7561 ccgggaacat gcaggacccg ccaggtacgg tggccgatcc gcagcagttc ccggtcctcc
     7621 agcgtgcggc tgaccgggaa gtcgggcccg caggcatcgc gaagtgcgcg cagggattcg
     7681 ggcgggtatg cggggccata gcgttcggta acaagccggt catgatcgga gatccagtcc
     7741 cgatcgagcg cgtgacagag gatctcggct tcgcgcagcg acccggcgtc acccagatgg
     7801 tcggcgtcgg cgtgcgagat gatgacgcgt gtcaggggcc cggggatttc accgcgagcg
     7861 agccgggact gaaccgaccc aggcagcgcc gcgtcgaaca gcacgcaacc aaaatcgtcc
     7921 tccagtacgt agaactggac cacgcgcggc cccatggcat cggcgagacg gatcacgccg
     7981 tctgtcatcg ccgctccttg tcgcggaaca gcacgtccca ttcgctctga aaggtgtact
     8041 cgaaaacatc gcgaagcaca tcctcaggac gcgcctctgt ttcgcctgca aggtaagcga
     8101 tgatcgggtg gtgcagttct gccaggcgcg agcaatcgaa cccggcttgc gaaaggacga
     8161 tgtccgcgaa ggcgaatacc ggtccggcta tgcgctgtag cgtttcggcg agcagcgcgt
     8221 tatcaccggt ttcccaaacc agcttgtgaa aagcaaaatc ggtgttggtg aatgcgcgga
     8281 cgtcgccccg gccggcggct tcgagcatcc gggcatgaag ttcccgaagt tctgccatga
     8341 tctcgggggt gaccttaggc cgcgcgagtt ccaacgccca gctttccagc cggtatcgca
     8401 tgctcatgat ttccgccaac tctgcctgag taagcgaacg gatgactgca ccgcgattcg
     8461 cctggacagt gaccagcccc tctttttcca gcagcagaat ggcttcgcgc accggtgcgc
     8521 ggctcacccg caactgactg gcgaccattg cctcttggat ggattccccc ggacggaaat
     8581 cgccgcggta aatcgcctcc cgcaaattgg aagcaatctg ggagcggaga tttgtggtct
     8641 ccaggggttt tagactgact gccatattgt ttttcaactg tacaacagat tagacggttt
     8701 ttcaattgtc gacatatcaa ttgttcaaaa tttcattgac acgccctgcc tctcgatgta
     8761 cactcctgga acagtacaag gttggttccg aaaagagagg tttgtgatga agcactatac
     8821 gcttgttgcg atgtctgtcc tgtgcctctg cacgatggct gtttcagtgc ggggacagga
     8881 ttatcgggcg aaactgtccg gagaggtgac cgatccctcg ggagcgtcca tgccgcgggt
     8941 gaaggtagag ctcaccaata agatcacact ggctgttacg agcaccgaaa cgaacgagtc
     9001 cggactgtat caattccagt ttctggagcc cggcgagtac cggatatctg tatcgagctc
     9061 cggcttcaag actttcgtgc aggacggaat ccgcttgcag accgggcagc gcgcggcaat
     9121 cgacatccgc ttgcaggtgg gtgacaccgc agaaaggatt gaagtgacgg ccgaggtggc
     9181 agcgctcgac acggaaagtg catcgcgggg gcttgtcgcc aacacgctgt tggttcagga
     9241 actgccgatc cgctcgcgca acccgctcaa tgtcgtcaat ctgcttcctg gaacgacaca
     9301 gcgtggaccg ggtgttttca tggcgtcctt ccacaactcc gccaacgtca acttcgccat
     9361 cagcgggggc agcccgcagc agaacgagat cctggtggac ggcgcgccga acacggtacg
     9421 caccaccggt gtgcagaaca atgttgcgct catgccggtt tcggagtcgg taggtgaggt
     9481 tgccatcgtc acgaacgcct acgacgcctc ctatgggcgc acgtcgggcg gcgtcgtgaa
     9541 cttcacgacg atggggggaa cgaatgagca tcacctgacc gggtggggat tcctgcggag
     9601 gaaaggctgg aacgcgaatg cttacccgct gaacgcgatc ggcagcccgc gtcccgaaca
     9661 gcaggtaaac cagtatggct tccaggtggc cggcccggtg gaaattccaa agctcatccg
     9721 gaagaatggc aggacgagtc ttttctacct ggcttcttat gagagatacg gagagctctg
     9781 gccccagccc atccgggtaa gcgttcccga accggaaatg cgcacgggcg atttcagcaa
     9841 actgcgcaac gccgcgggcg atctgatcca gattttcgat ccgttcaccg ccacgcccga
     9901 cgcttccggc aatccagtac ggcaggcgtt tccgaacaac gtgataccgg catcccgaat
     9961 ccatcccgtc gcggcggccg tgacaaagta ttatcacgac ccgaacgaca ggggacttcc
    10021 caaccagcgc tactcgcagg gcaacttcag cctgccgcta ttctcgtacg atttgaactt
    10081 ctggaactgg aacagccgcg tcgacgccaa cctgggagag atgaaccgcg tgtttttccg
    10141 gtattccacg aacaagcata cgcaggagcg tacgatcaac gggattatcg gccggccagg
    10201 cgagcaggcc tacaatccgt tcctgcggcg caaccatgca ctcatgctcg actgggtccg
    10261 aattatcaat ccttccacga cgctcaacct tcggggcaac tacgcccgct atatcgaggg
    10321 cttcgacagc gccggcaact tcggtttcga tctcacgacg ctgggtcttc cttcctcgct
    10381 cgtccgccag ttggcggtgc cggatttctt cggagtctgg gccatcggcg gctatacgca
    10441 gctcggcttt gttcccaacc tggaatacaa caacacgtat tcattgcaat cgaacatcac
    10501 caagatctgg cgcagccaca cgatcaaggc cggcatcgat gtgcggcgct ggcacttcct
    10561 ggtgaacaat cccgtgcagc cgttccgcgt gctttcgagc gccgctttca caaggcaggt
    10621 gtggaacaac tcggccagcg aggtgaacag cggtgacggt ttcgcatcct tcctgctggg
    10681 cgccgtcggc ggcggctcgg cggatttcgc cgtccgcccg ttttatcgct cctggtattt
    10741 cgcaccgttc attcaggacg actggaaggt cacccgcaag ctcacgctca acctcgggtt
    10801 gcgttgggac tacaacccca cgtcggacga gaaatacaac cggctggtaa ccggcttcga
    10861 ccggaccgcc gtcagccccc tggcctcgac atatcctcag ttgggcaact tgcaaggtgg
    10921 ccttacgttc gcgggcgtga acggcgaacg gcgccaggcg tccaaccccg atttgaatac
    10981 gattcagccg cgaatcggag ccgcctaccg catcggcgag gccacggtcc tgcggggcgg
    11041 ctacggcctg ttctacgcca actggccaac cggcgattat ctacagtcac aggggttttc
    11101 aacgagtacg gcattggtat cgtcgctcga tgggagccgc acgccccgga gcgaagcgct
    11161 gacagatccg ttcccaggcg gcgtccagca gccgatcggt gcggggttgg ggctgaacac
    11221 ctttctcggc tccaacttca actggtggaa ccaggacgcg cgcctgcccc gggtacacca
    11281 gttttcgttc ggcgttcaac gccgcctgac gtcgtcgtca tccattgatg tctcctacgt
    11341 cgggagccgc acgcaccatt ttcataccag cctgccggcc aatctcgttt ccgacgattt
    11401 catcgcccag tgcgatccct cgcgtggcgg gaaccgcagc tattgcgatg ccctggtgcc
    11461 gaacccattt gtgggattgc ccgaattcgc cggcaccagc ctggggcttt cctctaccat
    11521 cagccggctg cgagcctccc ggccgttccc gcaattcgac ggcgacctgg tggagctggg
    11581 ccgcagcgac ggacggatgt ggtacaactc ggcgcaaatc gtttaccgcc atgtgttcca
    11641 gaaaggcctg atcgtgaatg ccaattatac gttctccaag caggtctccc aagaaggctg
    11701 gctgaacaat tacgccgcaa ttccgcagcg gagcctggtg agcttcgacc gtacccacgt
    11761 actgaaggtg gctgcccact acgagctccc gttcggcaaa ggccgaagat tcggtgccgg
    11821 cgccgctccc gtgctggagc gcgtcatcgg cggctgggac ctcaacgtct tctacaccgc
    11881 cagttccggg gaacccgccg atcttccagg aaacgcgatc atgctaaagg accccaagat
    11941 cgaggcggac cgcggcgcta caatcgtgcg cggctggaac ccctgcgtac tgcaaaccaa
    12001 tgccgatgga agcatcgttc ccacgcgcgc gagcacggta gtgaacggat gcagccccac
    12061 ggatttttcc ggctacgcgt ggctggttcc ccggaacgag atgctaacct accggacgaa
    12121 tccgcttcgc agcggccaga tccgcatgcc ggcacagtat ttcgccgaca tttcgttgaa
    12181 caagaccgtg aatattaccg aacggctccg gttccagttt cgcgccgaag cgttcaacgc
    12241 cttcaaccgg ttcaatgtct tttccgtccg ctacaactcg aacccgctcg acgccaacgg
    12301 gaacttcggc agttacctgc cgagcgacgc gggagcctca tcgggtccga tgcgcgactg
    12361 ccctccgcgc tcgatacaac ttggcatgaa actgatgtgg tagacgctca tgttatcgac
    12421 acgacgaacc atccttcttg gtggcctttc gcagttggcc tggcctgccg aagactatac
    12481 cctgcgggcc ggacgcgccg aagctaggct cgcgcctagc ggcggattgg cggacttgcg
    12541 cttcgaacgg acaccactgg tttcttctgg actcggcgac ggttgtccgg aaatttcggt
    12601 ggacggcaag ccgatccgct gtgaccgccc ggtaaaggcc agccgcacgc gggacagtat
    12661 agctttcgaa taccgtatcg cgaacctgga ggttacctat gaggtggcgc tgcgtgaact
    12721 gaaggaagat gcggcgatgc tcgttcagcg cgtggcctgg aaaagcgatc agcgttcttc
    12781 cggcgcgatt gggttgcacg tgccgcggaa cgtcaggctg ccggcagcgt cgcgaagcat
    12841 gttcgttcca acgcgtgaag gcgtgggcag gcgtatggcc gtgccggatt ccaggcagcc
    12901 gccctggctg tacctgatgg ccggcggaga cccggaagcc acgaaacagc ggctcgcggc
    12961 gccggtgctt tcggagtact ccacggcgga caccctgcgc ctgacgattg catcagagcc
    13021 gggcttcact acttcctttc gggttgcggg agcatcggaa cccggctcgt tcgattgggt
    13081 tcacacaacc ggcggagaag ccgggcaagt gacggcgcga accttttaca cggtgattca
    13141 ccgcggcgcg gaccgccgcg cttattacgc tctttacgac tcggccctgg cgaatgtacg
    13201 gccggggccg gcgtggctgc acgacatcac gatggtcagc tacgactatc tgagcaagaa
    13261 cggccgcggg tggttcgccg acatcgacgc gctcgctaaa ctgatgcccg tttccgagcg
    13321 gaatcgggtc gtcctcacca ttcatggctg gtacgactat gtgggccgct acatatttaa
    13381 tccccgctcc aagacgctcg accgcacatg gaccgcattc tccaatgtga aaagccccca
    13441 gtttctggcg cacgcgcgcg gtccgcggtg gaagacagcg actcattggg gatggcgtaa
    13501 gatcctggac ggcttacggc cagtggagat gtccatcgcc gatctgcgcc ggcgcatgcg
    13561 ctatgcgcgc gagcgagggt tccgctgcat tctgtatttc ggcgacggtg tgaactcggg
    13621 cgacggcctg gcggatgtcc acgaaccgga gaaggttctt gcgtggggag gctggatcgg
    13681 tcccgagact tcgggaaaaa cctacgccca gaacccgctg catcccggcg ttcagtcatt
    13741 cttcaaggac tacctggcgg cgctgctgaa cgagttcggg cgcgacctgg acggtttcgt
    13801 ttgggacgag acgtttcacg tccgcgccgg ccagaagggt acggatgcgg tgcccgggta
    13861 cgcggatgcc gcgatgatga acctggtgag cgatctgcgc gcggccacgg agcgccacaa
    13921 ccccgagctc gctttcctca cgtcggatat tgtcggtccg aactatcagg cgccgtatgc
    13981 gctcgtatca catggcactt accaggactc catgtccggc ctgcgctact ggccgtacgg
    14041 gctgtttccg aacctgcgta attcgctgtg gtcgtgcaac tggtatcacc agacaacgtg
    14101 ggaccgcaac gagaagacag tggccacgta cgaactgccg gttgcgatct cgaacggata
    14161 cggtgatgat accggccctt cggacatgaa cgacgaacag attcgcaaga cgatggcgct
    14221 cttccggcgg ctgggcaacc gcaagcttga gttggagtgg attgaagaaa gcggcgcacg
    14281 gagaacctat aaaggtcgcc cggtgcccac cgagtgacgg ttatcgctcc gcttccgcgt
    14341 tagccgaatt accggctgta aatcactacg cccgctgcaa tcagcaccag cgcgatgagg
    14401 ctggcaagca gaagtccgct ctgaagcagc gagttttcga cgacattctt gaccaggcgc
    14461 tcttcgttct tcacccggtt tgcgaggctg tgcgtcataa ctccagacct ccttccacga
    14521 gcaccctcat tttaggactt tttcgcccga agaaccagca ataaaaagca aggcgcctgg
    14581 ctccgttgag agtgcgacaa tcgggggggg cgggctaccc attggagaga tgaggtgaga
    14641 aagacctttt tgcaaccgga aagggacgag cgcgatacgg ttccgccgtt gcgaactgcg
    14701 gcggagcacg ctttctcgcg tgcggccggc gctccgctga ttggaggcaa ccacgtgcgc
    14761 ttgctcaaag acgcgcggga gaactatccc gcatggcttg acgcaattgc cgcggcgaaa
    14821 caccacgttc atttcgagag ctacatcatt cacgaagatg aacagggacg cgagtttgcg
    14881 gacgcgttga tcgcgaaagc ggcggaaggc gtgcgcgtgc ggcttattta cgactggctc
    14941 ggcggcttcg gcaagacatc caagcgattt tggaaccgtc tgcgggcagg cggcgtcgag
    15001 gtgcgctgtt ataacccgcc gcgcttcgag agtccgctcg gttggttgtc gcgggaccat
    15061 cgcaaaatgc tcgtcgtgga cggcgaaaca ggtttcatca ctggactctg tgtcggccag
    15121 atgtgggttg gagatcagcg ggaaaacagg gatccgtggc gcgacacggg agtggaagtg
    15181 cgaggctgcg cggtggccga catcgagcgc gcgtttgcgc agatctgggc gctaaccggc
    15241 gagccgctgc ccgaggatga ggcaagcggt ttggaaacac ccgcccccgc cggcggcatg
    15301 atgatgagga ttgtggccag cgtgccagcg acggcgggta tgctccggct cgatcagctt
    15361 gtgtccgcgc tggcccggaa caggctatgg ctgacggatg cctactacgc tggaactact
    15421 ttatttgtgc aggcgctgag agcggcggcc aaggatggag tggatgtgcg cctcctggta
    15481 ccgggtggga cggatattcc cttgctacgg ccgttgtcgc gcgcgggcta ccggccgctg
    15541 cttgaaagcg gcgtgcgcgt cttcgaatgg aacggcacga tgttgcatgc gaaaaccgcg
    15601 gtggtagacg ggcactgggc gcgcgtcggc tcgacgaatc tcaacgtggc gagctggctg
    15661 agcaactgcg agttggacgc cgtaatcgac gatgaatcct tcgggcgcga gatggaagag
    15721 atgtacctgg aggatttgag caactcaacc gagattgttc tgaacgtgaa acagaaaatg
    15781 cgcgcgccgg gcgctccgcg ccacccgcac ctgggtcagg gtagcggcag cgttggacgt
    15841 gtggcagcgg gagggattcg gcttggcagc gcgatcggag ccgcctttac cgaccgccgc
    15901 gtgctgggcc cggtagaagc gcagttgatg ctgatggcgg ggctgctgct gatatcgttg
    15961 gcggttctct ttgcgttctt tcccaaagtg ctcgtttacc cggtgatcgt cttcatgacg
    16021 tggatggcgc tggcgctggt ttacaaagca tacagattgc gccgcaacag gaagaggcag
    16081 gagcaacggc cgaatctata gaatttccgg tgcggctggt tgtccagccg caccggatag
    16141 gagatcggac taccaggtca gccgcaatcc gaactgaagc gagcgggcac cgttgcccga
    16201 cgtggtggcg tcgatgatgc cgctggtcga atcgggcgtc ttcgggatgc ccggcatgtt
    16261 gaacacgttg aagaagtcga tggtcagccg gaaaaagacg cgttcgttca caggaatgac
    16321 tttgaacaaa gaggcgttct gataccagtt aaacaggccc tggccgtact ggtttcgcca
    16381 ggggtttaag ccggtgtcca aggggatccg ctgcaggctg ccgtctttca tcggcaccca
    16441 tacgttgttg ctttcgtagt aagcgaacat cgggtcattg cggtcgccgc cgtcggcagg
    16501 cgtgggaata agaggactgt gcgcgggttt gtaattggac ggcactccca tcaccccatt
    16561 cggccgtcct tgcgcgtcgt agctgttgat ccggttcgcg gggatatagc cgttgtagta
    16621 gagataaccg tcccggcaaa cgccgctgcg gcaatcctgg atcgggtact tcgtgccata
    16681 gatctcaaga tcgttgggga agctccagtt cgtggccgga agtgcccacc acgtgctggt
    16741 gaacgatcca tctccggcga tctgccatcc gccgacaatg cgatccagta cgggacctgc
    16801 gctggatgca aatcgcttgc ccttaccgaa aggcaaatcg gcaatccagt tccagttgaa
    16861 gcgatgcttc ggaatgtcgg tatcgcgccg gtagaacagg aaacgagcgc gctcgttatt
    16921 atcttgtggc acggctccag gcaggtagat gctggcggga ggtagaatgt catcccccca
    16981 gccgttgccg cccgccttaa ggctattgct caggacatag aaaaactgga agccgtagcc
    17041 tttcgaatac cgccgctgaa cctcaatttg aatattctga gagttcgacc accctgtttt
    17101 ctggtactcc tcaatttccc cgaacatggt ggtttcgaaa ctccgccggg cggtgccggc
    17161 gtaggtgccg gtaggcagag gcaccccctg cgtagcgaac cagacgtagc tgttcggcgc
    17221 ctggttatag gaatagtaca tatccaggcg cgaaccgtgc gttgcggcat agcccacccg
    17281 caaaagggtg ttgtctatca cttccttctc gaaggtcagg ttccactggt gcgccctcga
    17341 cgacggctgc tccggattga agtagcttgt caggaacgaa ccacggctga cgccgcccgg
    17401 ccggttcaaa tcgagaacgt cacggctatt gactccggcg acatactgcg gcaccgccct
    17461 caagccgtag gcgggcaagc ggtccggcgt ctgggaggag tccccgaggg aattcgtgaa
    17521 acgagccgtg gtgggcggat tttgccgcat gcgggcgttg aaatcccgca gaggcatggg
    17581 ataagcataa atcccgtagc cgcctctcag cacgagtgac ttcgcactat cgctgaaccg
    17641 gtaagcgaag cccgccctcg gaccaaagtc ccacttgttc aagtggatca ggccttgcgg
    17701 taagccgaca tcctgcgggc ggacaaactg gagccccatg tcggtgtata ccttcgtaat
    17761 ggccggcgtg gttgcattgg cccggtacat cgtctcccaa tccgctccat ttacgacgga
    17821 ttttgtcgcc gggtcgaagc cggtcaaaat attgtcggct tcgcgaatgg gcgtatacat
    17881 ctcccagcga aggcccaggt tcagagtgag ccgggaattc actttgaagt tgtcctgcaa
    17941 gtacagcgag atctcgctgt cgtgcaggtc gtaccacttg cgaacaaatt gcgccgaata
    18001 gctgcccatt actccgatga acagattcgc ggcgttgtgg ccggtgtacg gaacggcggc
    18061 gtaagtggaa cccgatgcgg gatcgtacag gccggtggcg ccgctcgagg cggcatgcgc
    18121 gccctgaacc tgctgctggt ccggcagcac gttcatcttc tcatagcggt agcggccgcc
    18181 gaacatcaac tcgtgccggc cgtgaacttt ggtgaagttc tgatcgaagt tgtagatgtg
    18241 gatgatgctg ttgcgccggt ttgccgccgt gatgtactcc atgccgacgc cggtgctggt
    18301 aatgttgggg aacccgtatt cgtcaaacgg attaggcaag ccgagtttgt cgccccattt
    18361 ctcggtatcg cctacgttga tcaggttcag gtcctcgacc gccacattga acgatgtttc
    18421 gctgaagaag gtaggcgaaa acgtatgcgt ccaggaaacc actccggtat cgttccggat
    18481 gggccggaag gtgccgttag cggcgaaatc gagcgtcgtt ggcgagttgt tgggtccgga
    18541 ctgggcatag gtatcccgta caccgtggct gtagcggaag aaaatctgat cggcatcgga
    18601 gatccgatga tcgatccggg tggtggtggt ccactccatg cggttgtttg gcgcctggta
    18661 gaagtagttg tcggcaagca gcgggttcac ccccggctcg gtcggcatcg gcgtcacgct
    18721 gtacaaatac tttgcgagcg gactctggcg ggacattggg atctggttgt ttgggaaagg
    18781 ctgccggctc cagttcgcat ccgacgtcca cgggtcgtat aagtcatagc ggcgtcccga
    18841 gccatcgaca agtccactga agtcgccttg ccgcatttcg gcggtaggca ggcgtgccag
    18901 tttggtcgtt gcggtccggc tgcgaaatgc ttcgtaagcg gaaaagaaga aggtgcggtt
    18961 gcgcccgtcg tagaccttcg gcaagtagat cggccctccg gcggaggccc cgaactcgtt
    19021 ccgtaccagg tggggaggcc tgctccattt gtcctgccgc gcgcgagcta cgccgaaacc
    19081 caaattgttg ttgcgcacga tttcgaacag tgaaccatgg aattggttcg tgcccgcgcg
    19141 ggtattgacg atgacggttc caggccggtt catcttcgcg gaagagttgt ttgtctctac
    19201 gcgaaattcc tcgatggtgt ccatccccgg aggacggccg gcaatttcgc cgatgtcgcg
    19261 gttggcgagc accgcgccat cctggaggaa ctcgacaccc catctcagcc cccagacgcg
    19321 tggggtcatg gatgcgccgt caatgcccgg tgtggtgcgc ccgaccagag actgaaacat
    19381 gcgcccactg atgggcaact ggtcgatccg cgcacggtct gtcaccgctg ctagagtcgg
    19441 gcttgtagta gtgaccaggg gtgcggcatc cgcgatcacc gtgacttcgg tcgccgtaga
    19501 cccaacggtc atagtagcgt ccacgacagc ggtttcaccg gtctgtaaga gaaactctcc
    19561 cttgaaattc tccatcccgg cggcttcgat cataatgcta tagcg
//
LOCUS       DASTMK010000045        19942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_66721, whole genome shotgun sequence.
ACCESSION   DASTMK010000045 DASTMK010000000
VERSION     DASTMK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19942
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66721"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3257)
                     /locus_tag="VFQ79_01900"
     CDS             complement(<1..3257)
                     /locus_tag="VFQ79_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU0138427.1"
                     /translation="MGLSKLLAAYLILAIFVAPGLQAANVFVLPGPSNVNQTVTMLTP
                     VSLFQVGSIMQAPNAFKVFSKPDGSRLYIVSSSAVDSLVVADAAGNVIARHSLGAITA
                     AELSPDGRKLAIAAATAYLLDTTVDTAPVAVNLGFVPSDVAVSVDSTRAFFVGSGTTQ
                     VSAVDLITNATVAFVTLPQSGANVATAPGGWVYVSSSNAVYEIDGRGTMAIRAEMMAS
                     GQPGKLQFTPDGLRGIALNRAGFGAGPVSILDVSSRMVTPVSSLGQIFDANAAGKLAV
                     TSNSRAFAAGSDGRLYSITLNPPAITPVVASVSGITSVVASEEKPNARYLFFLSPTTL
                     YRSATSLFTTDGLDTPASAGVAGLPAVLAYAGAATGVFPAGLTAVGPSPIVPPGMTTM
                     LTVRVIDVTGKPVYNTPVAFATDGIGATILNPAVNTGMDGLARTNVTAPPFAGAFTVR
                     ATAGAGLSVSIGVAVGEGGETGTGVTAISGQGQLVNAATAGRQLVVQVRDSEGNPVGG
                     TQVSWSPGVIGIGGAPVTVTSHQDLTDALGRAVATFSTSPLQSAELAAATIVANTLYG
                     TATFYITAFPTFTLGSPPAPIPPPAVTFSFGSQTGVLEGRAGQTITNAVAVQVLRQAG
                     VSFGSGIPNVGLTAGTSADPATGPTAACAGGTVLTDAQGVAVCNLVLGPRPGETQLTI
                     DVGGYMQRTFALRIQAGVPAEITPIQGNNQSGSAGQQLPQALVAEVRDSLGNLLPFAT
                     GIQWEAVNQCATLTGSSTTANNQGRVQTLVRLGNVAGSCEIRVTTAEASATFTVQVAS
                     AAGQVTAISGGGQAAILDQAFAQPLVAEVRDAQGALLPNTPVSFAVTSGSATVSSSSV
                     TTNAQGRAQVTVTAGASTGPVVITATSGTGSATFSLTVVPVGPSVERAGIVNGASFQQ
                     GVVPGGIVTIFGTNIVPGIQGTREANPFIGSLPTNLEGVEVLFGNTPAPIFAVANQGG
                     REQVSVQAPFDLPAGGVTTITVRAGAGSSTISDVPVLAVQPGLFETIPRGSSRAYPVI
                     TRADGSFVTPQNPARRGEILQVFATGLGAVTPSGVTGRAGVPGQEVVAPVIVGVN"
     gene            complement(3390..5159)
                     /locus_tag="VFQ79_01905"
     CDS             complement(3390..5159)
                     /locus_tag="VFQ79_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138428.1"
                     /translation="MADFRKWLPVLALVALMLGSMSTASAQTAFSCSATTGAVTPIIR
                     GEGITELVGDIVLQCTGGVPAADPAAVPQVNIQIFLNTQITSRILDSNSTTEATLLID
                     DPAVGARQLGPIPGVVPAGANNTYLARRVAENAVAFLGIPVDAPGTTGTRILRITNLR
                     VNASGIGVSTSFIQNQAIAFISISGATTIPVNPAQLPVAFVSRGLDFSLAAHARNLRQ
                     CVAVNRDLTSPDPDESGSINAVLEYEEGFPTAFKTRGALTGGAIVQNLIGTNYNTESG
                     FTLDALNAAAGIANTGTRVMARFNGLPAGVTAYVTTTNTNQATPATDGLVAAYVSGTD
                     LNGAGGFPDASGIIKGGTAGGWDLVQVNTVNGAGMAVWEVTGVGPQSNSTLESLTFGV
                     VFAYTPNFANNVPALGTGTVTGSFAPLSVVTSASQNQPIPRFVGPGTSADIIRIAACV
                     TNLLFPFVTNQAGFDTGIAITNTSQDPFSSSNAQGGTCVLNFYGGTTAAPGTNPPAQT
                     SQAINAGQQLVLSLSAGGNLGAGPVAGFQGYIIAQCNFLYAHGYAFISDVGAQNLAQG
                     YLALVMGPGGETRGLAAAEALNQ"
     gene            complement(5645..8005)
                     /gene="gspD"
                     /locus_tag="VFQ79_01910"
     CDS             complement(5645..8005)
                     /gene="gspD"
                     /locus_tag="VFQ79_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEU0138429.1"
                     /translation="MKVLSSRIAALMALPLLFAQEIPPPPRGLPPGFGPIYRPQAEKQ
                     EEPKQPPPQAQPQTGTQQSPQPPAAAAKPQGPTATTPQPSPPGAVSFGGLSLQNASLS
                     QVIDQLARILKINYILDPRLKGAVVLNTYGDTRDLDPRNLLELILRINNAGMVQVGDI
                     YRIVPLDQISRLPLEPTTDIQQIPENDRTYLNLVFLKYATVDELVKVLQPFMGENSTT
                     VTYPPANLLLILDSGRNLNRLMELIALFDSERFASQRVRLFEVTNGRPSDIAGELESI
                     FKGISLSEKNTPIKFLPLDRINTLIAIAPNPGVFEEVEKWLKRVDVAVKVTAGAVDNY
                     VYRVKYGRAEGLAGSIMMLYGGYGYGMGGYGMGGYGMGGYGGMGGYGGMGGYGMGGYG
                     GMGGMGGYGMGGYGMGGYGGMGGYGMGGYGMGGYGGMGGYGMGAPIPTTFATPGTPLP
                     GTVTGQGTQAGAATTGSADQTGTYLGASSYGMAGGRIPRVVPNPMDNTLLIQGTQQEY
                     ESILKILRELDIPPRQVLVDAKIYEVSLTGALASGVSAFLQRRGESGGAAVPTNQFRA
                     KLDGGVLNFTIGTLVGQSRELLAFLEARESATFTRILSAPSVIATDSIPATINVGSEV
                     PTLTAQAATGVQTGGSSLFAQTISNRNTGVTLAILARVNPSGIVTLVINQEVSAPEPP
                     PTEGINSPAFSKRAVQTQVTVQDGDTIAIGGIIQEDAGATSSGFPLLHRIPGIGAAFG
                     SKSYSKGRTELIIFMTPRVIYDTNQMRDASEELKSRLKKLQRYVEE"
     gene            complement(8009..8500)
                     /locus_tag="VFQ79_01915"
     CDS             complement(8009..8500)
                     /locus_tag="VFQ79_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138430.1"
                     /translation="MRLLILLAISVPFVCAAGEGSGKRKTESRKAAPRASTSVRKAEP
                     RATPQLTIPPDAKQVGSNSYLYTDSNGKVWTYTRTPFGVRKEEGAPESGATTQDVPPE
                     MTAAEEGDSVRFTRQTPFGKSQWTRKKSELTGVERAVWERDYPPKPAADQPDEAASGS
                     VKE"
     gene            complement(8515..9234)
                     /locus_tag="VFQ79_01920"
     CDS             complement(8515..9234)
                     /locus_tag="VFQ79_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138431.1"
                     /translation="MKSKLWLLNIVLLALVLWLGSRLRQDWLAARERETKLLEQTVKA
                     LPAPPIAPAAPVNPVTAGSYAEVAQMMLFSKDRNPNVVIEAAEPAPMPALPVAHGVMD
                     FGGGPTVFLSEKPGGPQRGYQVGEAIGQFKLLAANNREIAFEWNGETVTRTLDELMDK
                     SKPETAEAAPPPAAAGSKVTSATTVSAGPAASGPGADIGADLKACQPGDTSPSGTVQG
                     SMRKVVTKTPFGESCRWEPVK"
     gene            complement(9231..9824)
                     /gene="gspM"
                     /locus_tag="VFQ79_01925"
     CDS             complement(9231..9824)
                     /gene="gspM"
                     /locus_tag="VFQ79_01925"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEU0138432.1"
                     /translation="MTIGQRDKRALAILAMAVAAIVLFQVLSRDSSPAAVVVVADSVP
                     AAEKRLTRVRQMAATTPGKQDALKQVAAELAQREKGLIQADTAAQAQAQLLQILRRIG
                     RAQTPVVDIRGNEFAQPKPFGDDYGEVSVSVSMECGIEQVVNVLAELTAQPELIATTD
                     LRIGATSNKEKLVPVRLTVSGLVPKRLLPEKKGPGQF"
     gene            complement(9821..11149)
                     /gene="pilM"
                     /locus_tag="VFQ79_01930"
     CDS             complement(9821..11149)
                     /gene="pilM"
                     /locus_tag="VFQ79_01930"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEU0138433.1"
                     /translation="MAVSTKIRRWLAFGTGVAIEISGDDLHVLVTRVRPTGSQILGSA
                     TIEKYAARPASEWGAEYAAFTKKLGAGHIAAAVLLPRAEIIVRQLNLPGVPDRDLEAA
                     IALQVDSQHPYPEDDVVYAWARLGSSSSILIGIARRETVDRYTSLFAEAGIKTASFTF
                     SAAAIYSAIRLLSSPPADGFAVIRPFNGSVEIYGESEARAVFSAEVRGSEERARTLAL
                     AELRLEPDSETGHLRELLPLPKDAPSDDEEYRRALGERALPYAAALAGACPRLALAAN
                     LLPAGRRSTSSRLMYVPTIFLGACLLLLLSALAVHGKYRERRYLSVLESEIAKLEPHA
                     RQVAALDRDTQLARRRTALLDNFRRRSKHDMDVVNELTTVLAPPIWVSQLEVTRAFVH
                     IAGEAEQAAPLLKVLDESPLFQDSQFTMPLGRTGTGEVFRIRTTREGVPE"
     gene            complement(11140..12144)
                     /locus_tag="VFQ79_01935"
     CDS             complement(11140..12144)
                     /locus_tag="VFQ79_01935"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138434.1"
                     /translation="MREWSVQRGGALLAVLWLSAALSAIAFSVANSVRGETERASTHA
                     DSVRAYYLAAGAVDRALLYMQWGPQYRNPDGTPRYYGPDKPFLRMPFPTGEVFVEVIP
                     ETSKLNINTAPAEELLRLMLAAGIPPGQADEIVAGIIDWRSAVPPGAVTLFDQYYLSL
                     APSFRARHASFQEIEELLLVKGMTPDLFYGSYERGPEGNLIPRGGLRDCLSVYGALAG
                     FDVNTVSPVLMAAIGLTPNSIAAILERRRVLPFRSPADLAPLAEAGGPGFRKLAVSAG
                     TIFTIRATAQLRIGENRVSDLRRSVAAVVKYPADFAKEPPYTVLRWYDNTWVDLRAWQ
                     "
     gene            complement(12203..12934)
                     /locus_tag="VFQ79_01940"
     CDS             complement(12203..12934)
                     /locus_tag="VFQ79_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU0138435.1"
                     /translation="MKRASRGVTLLELLIAVSLVGLVSLGILMAIRVGFGAMEKSNAR
                     LMENRRVAGAQQILEQQIAGFMAVQGDCVETPDRPPTRMSFFQGDIESMRFVSSYSLQ
                     EAARGMPRLLEFQVIPGENREGVRLVVNEHLFAGSLSTAPFCFGLGPDPITGAPVPRF
                     PPVQVGPRSFVLADKLAFCRFMYREPAPPPEFERWTPRWVRADLPMAVRVEMAPLRPD
                     PSRVQLISLTAPIRVTKDPMVTYEN"
     gene            complement(12931..13359)
                     /locus_tag="VFQ79_01945"
     CDS             complement(12931..13359)
                     /locus_tag="VFQ79_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU0138436.1"
                     /translation="MNQRGFTLLEVLVATVIMAVAVAGLLSSLSTSMRSGSRLIDYDR
                     AALLARTKMDELLVQSRLPRLVVIEGALEAPEGMKSGWRARVTPFEMPPKAGPGTPIL
                     ERVELEVWWTAGEQRRTFTLEGYRRGTLTREDIVAGGVVQ"
     gene            complement(13356..13817)
                     /locus_tag="VFQ79_01950"
     CDS             complement(13356..13817)
                     /locus_tag="VFQ79_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138437.1"
                     /translation="MTLMEMMVVAALLALLAGITFPSVNSGLDSLRLSSATNSIVSFF
                     NSGLNRAQRRQQVMEVTIAPGENLLRLRSSEPGFLREMTLPDGVEIAAVLPPLPYESP
                     HQSEDARRFMLYPGGTVPRFGVEIVNRRGARRIVRVDPATGVPQVERVEQQ"
     gene            complement(13922..14356)
                     /gene="gspG"
                     /locus_tag="VFQ79_01955"
     CDS             complement(13922..14356)
                     /gene="gspG"
                     /locus_tag="VFQ79_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEU0138438.1"
                     /translation="MKKRTERRQAGVTLIEMLVVVTIIALFAAVVAPRMLQKTDTARI
                     TAARTQINSFMTAIGTYKLDTGTFPTTEQGLQALRTRPQNVNQWQGPYLPAEIPVDPW
                     GRPYVYVYPGEHGDEPDVISYGADGQPGGEGINGDVVSWKTQ"
     gene            complement(14382..15590)
                     /locus_tag="VFQ79_01960"
     CDS             complement(14382..15590)
                     /locus_tag="VFQ79_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0138439.1"
                     /translation="MASFHFRAVAADGKVRSGTLTADTEKLAASELRKQGLTPVYVGV
                     QQKKSFELKLPAMNRGKRRDVLFFTQELSTLLNAGVPLDRALSITSELTERPQFRFIV
                     LDILRVLKSGKSLADSLSTHPAHFSQLYVNMVRAGEASGSLGVVFEKLSDFERTRDDL
                     RNYIVSSMVYPALLAVVGLASILVLMYFVVPRFASVFEESRIEMPLPTKIMLETSRFV
                     QAYGWIGIASMVGVILAWSAYVRTSGGRLWWDRFRLRIPLLGDALRKAETARFARAMS
                     TLIANSVPLVQSLSIASAILNNSLIAGSLAQVAQGVKRGEGIAGPLKKTAQFPPLAGH
                     LLSVGEETGQLHQMFSRMADIYDADTRAAIKRFTALFEPLIILVMGIMVGALILSMLL
                     AITSINEVAI"
     gene            complement(15590..17227)
                     /gene="gspE"
                     /locus_tag="VFQ79_01965"
     CDS             complement(15590..17227)
                     /gene="gspE"
                     /locus_tag="VFQ79_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEU0138440.1"
                     /translation="MRLGEVLIERKLITGDELERALEIQKERGEKIGKILVDLGFIAQ
                     RDVLAALSDQLSLPLVAIDGPPPVTPETEGLSPRFLRQFRCLPVALFDSTVSLAMADP
                     LDLETISTVTAVTGLKVEPALAPEQEILDAIDKYYGQAEKAAEAEFEDGGQASEDLEQ
                     LRDMASEAPVIRLVNAMIAQAVEKRASDIHVEPFEKQFRIRYRVDGVLYEQDPPPREL
                     KAAIISRVKLMAKLNIAERRLPQDGRIKTKTLGREIDLRVSTLPTLYGESVVMRLLDR
                     SAGDFYDLRSLGFDDAMLARMEHFTSLPHGIFLVTGPTGSGKSTTLYSALKRINLPDK
                     KIITIEDPVEYQMDGINQIHVNPQIGLTFASGLRHIVRQDPDVIMVGEIRDRETADIA
                     IRSALTGHLVFSTLHTNDAPSAITRLTDMGVENYLITSSLVAVLAQRLVRVICPACKQ
                     PAGQRMTPDGNLVDTYRGAGCDSCFGTGYTGRVGIFEMMELNDEIRRLIMNNADAVDI
                     TAAARRNGMRTLREDGWLKVGNGLSTADEVMRVTQEF"
     gene            complement(17230..17772)
                     /locus_tag="VFQ79_01970"
     CDS             complement(17230..17772)
                     /locus_tag="VFQ79_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138441.1"
                     /translation="MRYAALLLCLALPVLSAQDTVRVGYTAIQPVEANFNGRIEKFDI
                     DNPIRLIGSSRGVYLDGYGVVLTAEVDLLPSASLTPFRPRYTDPQKEQLRVRKLERVD
                     RLRDVMRDMLVDTAAALDTVPLDQQIVVAVTIFYWEWERRQGLPSQILMQAPRRTLVE
                     YKAGGIARPALDAALKVREY"
     gene            complement(17769..18368)
                     /locus_tag="VFQ79_01975"
     CDS             complement(17769..18368)
                     /locus_tag="VFQ79_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0138442.1"
                     /translation="MNCSPDVLKDYLLGELPGPDCLTVEAHVRACPACSEELERLRTA
                     QAALASVPDVEMPRRIAFVSDRVFEPGWWQRLWNSAPRLGFVSAMVLAAAILVHGLTR
                     PAPAVPETAAYNQEAVEARIEAEVSRRIPAAVAQAVAEHDARLRTEMAKLVAASESKM
                     NFERRADLVTFEQAFTLLKKQVDILQYPRLASSEMVPSR"
     gene            complement(18365..18910)
                     /locus_tag="VFQ79_01980"
     CDS             complement(18365..18910)
                     /locus_tag="VFQ79_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0138443.1"
                     /translation="MIFREVEDRDLIHKARKGNVDAYNILVSRWEKRVFNYIYRLVGH
                     REDGLDVTQDVFLKAYQNLGKLEDPARFGPWLFRIAHNESYSLLRKNRPETELKEPPA
                     PAHNPRMLPIEVSIAIERAMATLSADQREAVVLKIYQGFKFEEMSEILSCPVSTVKSR
                     LYTALDLLKDALAPVKARGVE"
     gene            complement(18969..19445)
                     /locus_tag="VFQ79_01985"
     CDS             complement(18969..19445)
                     /locus_tag="VFQ79_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU0138444.1"
                     /translation="MAMLKRLQDFLDRSGAGYTHHVHPLAFTAKEVASAEKVAAHRVA
                     KCIVFFSDTGYGMAVLPADSWVNLQELRAALGVANIRLATEKELDELFPECELGAMPP
                     MGSLFGLPLYVDGSMAEEEEIAFNAGTHRDVVYMKYRDFERLNKPVTLQFARYLTA"
     gene            19570..>19942
                     /locus_tag="VFQ79_01990"
     CDS             19570..>19942
                     /locus_tag="VFQ79_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138445.1"
                     /translation="MISTLLFLLLLSQGIPNEQRLNQHRVTSRNSPAPEPGAENKKIP
                     SLVEGLRSAHDWYTKKRPELVKLWTTILGKTAPAPEDKQWFGDITKAVVHDEQDKGSY
                     TRIRLDLPMEKDFYQQHLLLLP"
BASE COUNT         3970 a         6321 c         5829 g         3822 t
ORIGIN      
        1 ttgttcacgc cgacgatcac gggtgcgacg acttcctgac ccggcacgcc agcccggccg
       61 gttactccgg acggagtgac tgcgccgagc ccggtggcaa acacttgcag gatctcgccg
      121 cgccgcgccg gattctgagg cgttacgaag cttccgtccg cacgggttat cacgggatac
      181 gcccgtgagc ttccccgcgg gatcgtctcg aagaggcccg gctgtaccgc gagcaccggc
      241 acgtcggaaa tcgtgctgga accggcgccc gctctcacgg taatcgtggt aacgccaccg
      301 gccggaaggt cgaacggagc ttgcacggaa acctgctcgc ggccgccctg gttagcgact
      361 gcaaagatgg gggccggcgt gtttccgaac agcacctcca cgccttccag gtttgtgggc
      421 aaggagccaa tgaaggggtt cgcctccctg gttccctgga tgccgggtac gatgttggtc
      481 ccgaaaatag tgacgatgcc gccgggaacg acaccctgct ggaagctggc gccgttgacg
      541 atgcccgcac gctccacact aggcccgacc ggaaccaccg tcagcgagaa tgttgccgac
      601 ccggttccgc ttgtcgcggt tatcaccacg gggcccgtgc ttgcgccggc cgtcaccgtc
      661 acctgtgccc tgccttgagc attggtagtg acgctggagc tggatacggt cgcgctgccg
      721 ctcgttacgg caaagcttac gggagtgttt ggcaaaagcg ccccttgagc gtcccggacc
      781 tccgcgacga gcggttgcgc gaaggcttga tcgaggatag ccgcttgccc gccgccggaa
      841 attgccgtca cttgccccgc cgcggacgcg acttgaacgg tgaaggttgc ggaggcttca
      901 gcggtggtta ctcgaatctc gcacgatccc gccacgttac cgagccgcac cagcgtttgc
      961 acgcgaccct ggttattggc ggtggttgag gagccggtaa gggttgcgca ttggttcacc
     1021 gcttcccact gaatgcctgt ggcaaaagga agcaggttgc ccaagctatc gcggacctcc
     1081 gcaacaagag cttgcggaag ctgctgaccg gcgctgccgc tctgattgtt gccctggatc
     1141 ggtgtgatct cggccggtac gccggcttga attcttagag caaaagtccg ctgcatgtaa
     1201 ccgccgacat cgatcgtcaa ctgggtttca cccggtctcg gtcccagaac gagattgcaa
     1261 acggcgacgc cctgggcatc cgtcagtacg gtgccacccg cgcaggccgc ggtaggtccc
     1321 gtggcgggat ctgcgctagt cccggcagtg aggcccacgt tgggaattcc gcttccaaac
     1381 gacacgcctg cctggcgtaa cacctggacc gcgacggcat tcgttatcgt ctggccagcc
     1441 cggccttcga gcaccccggt ttgactcccg aaggagaacg tcacggcagg aggcggtatc
     1501 ggcgcgggcg gactgccgag agtgaaagtc ggaaatgcag tgatgtagaa ggtagcagtg
     1561 ccgtacagtg tattcgccac gatggtggcg gccgcgagtt ccgcgctctg taaaggtgaa
     1621 gtgctgaagg tggcaacggc tcttccaagc gcgtccgtga gatcttgatg cgaggtcacg
     1681 gtaaccggcg cccccccgat tccaatcaca ccgggagacc acgacacctg ggtgccgccg
     1741 acggggttgc cttccgagtc gcgaacctga acgacaagct gccggccggc cgttgcggca
     1801 ttcaccaact ggccctgtcc ggaaatcgcc gttaccccgg tacccgtttc gccgccttcg
     1861 cccacggcaa caccaatcga cacgcttagc cccgccccgg ccgtcgctcg cactgtaaat
     1921 gcgccggcga acggaggagc agtcacattg gtacgcgcca gaccgtccat accggtgttt
     1981 acggccggat tcaatatcgt cgcgccaatg ccgtcggtag cgaacgccac cggcgtgtta
     2041 tagaccggct tcccggtaac gtcgattacc cggaccgtca gcatagtcgt cattcccggc
     2101 ggaacgatcg gcgacggacc aacggcggtc agacccgcgg gaaaaacgcc ggtcgccgcg
     2161 cccgcataag ccaacacagc cggcagcccc gcgactccgg cgctggccgg cgtatcgagt
     2221 ccatcggtcg taaagagaga ggtagccgag cgatacaggg tggtgggact gagaaaaaag
     2281 aggtaccttg cgttgggctt ctcttcggaa gctacaaccg aggtgatgcc ggaaacgctc
     2341 gccacaaccg gcgtgatagc gggaggattg agtgtaatcg aatacaggcg cccgtccgaa
     2401 cccgcggcga acgcccggct gttggaagtc accgcgagct tgccggcagc gttggcgtcg
     2461 aagatctgcc ctaacgagga aacgggagtt accatacggg aactcacgtc cagtatcgaa
     2521 accggtcctg ccccaaaacc ggcgcggttc agcgcaatgc cgcgaaggcc atcgggtgtg
     2581 aattgcagct ttccaggctg cccgctcgcc atcatctcag cccggatcgc cattgtgcct
     2641 cgcccgtcga tctcatagac cgcgttcgaa gaggacacat acacccatcc tccgggcgct
     2701 gtcgccacat tggctccgct ctggggaagg gtgacaaaag cgacagtcgc gttcgtgatc
     2761 agatctaccg cgctcacctg agtagtaccc gaaccgacaa aaaaggcgcg ggtggaatcg
     2821 acgctgaccg cgacgtccga ggggacaaag ccgaggttca ccgctacggg cgcggtatcg
     2881 acagttgtat cgaggagata agccgtagcc gccgctattg ccagtttccg gccatcgggc
     2941 gataactcgg ctgccgtgat cgcaccgagc gagtgccttg caatcacatt tcccgcagcg
     3001 tcggcgacga ccagactatc gaccgccgaa ctggagacga tatacaaccg cgatccgtcg
     3061 ggcttcgaga ataccttgaa agcgttcggc gcctgcataa tgcttccaac ctgaaacagt
     3121 gacacaggtg tcagcatggt caccgtctgg ttcacattag acggtcccgg cagaacgaag
     3181 acatttgccg cctgcagccc gggtgcaaca aagattgcca agatcaaata agctgcgagc
     3241 agtttagaga gacccattct ttgctcctga aatagaagtt ctggtgccga tgaggacgcg
     3301 ggaaaaacct tcttacactt atcacacttg agacacaaaa aaaaggaggg gcatttctgc
     3361 ccctcctttt cttcgactgc aaaatacgac tactggttca acgcttcggc agcggcgaga
     3421 ccacgagttt caccgccagg acccatgacc agagcaaggt agccctgtgc caggttctga
     3481 gcaccgacat cgctgatgaa ggcataacca tgcgcataga ggaagttgca ctgcgcgatg
     3541 atgtagccct ggaagccagc taccggaccg gcgccgagat taccgccggc cgacagcgac
     3601 agcacgagtt gctgtccagc attgattgcc tgcgacgtct gagccggagg attcgtgccg
     3661 ggagcagcgg tcgtgccgcc gtagaaattc aggacgcagg tgccgccctg tgcattggaa
     3721 ctgctgaacg gatcctgcga cgtgttggtg atcgcaatac ccgtatcaaa accagcctgg
     3781 ttggtaacga acgggaagag caggttcgtc acgcaagcgg cgatccggat gatgtcagcc
     3841 gaggttcccg gtccgacaaa gcgcggaatc ggctggttct ggctggcgct ggtgacgacg
     3901 ctcagaggag cgaagctgcc cgtcactgtg ccggtgccga gagccggaac gttgttggcg
     3961 aaattcggag tgtaagcgaa tacaacgccg aaggtaaggc tttccagcgt ggaattcgac
     4021 tgtggaccca ctccagtgac ttcccaaaca gccataccgg cgccattgac cgtgttcacc
     4081 tgaaccaagt cccatccacc agcggtgcca cccttgatga tgccgctggc atccgggaag
     4141 ccgccggcgc cattgaggtc ggtaccggag acgtaagcgg caaccagtcc atccgtcgca
     4201 ggcgtcgcct gattggtgtt ggtggttgtg acgtaagctg tcacgcccgc cggcaggccg
     4261 ttaaaccgtg ccatcaccct ggtaccggtg ttcgcgatac cggccgctgc attcaacgca
     4321 tccagcgtga agccggactc ggtgttgtag ttcgtaccaa tcaaattctg tacgatggct
     4381 ccaccggtaa gggcgccccg ggtcttgaaa gcggtgggga aaccctcttc gtattcgagc
     4441 acggcattga tgctgccgct ctcgtccgga tcgggacttg tcagatcgcg gttgaccgcg
     4501 acgcactgcc ggaggtttct ggcatgtgcg gccagactga agtccagccc tctggatacg
     4561 aaggcaaccg gcaactgcgc cgggttcacc ggaatggtcg tcgcgccgct gatggagatg
     4621 aacgcgatag cctggttctg gataaacgaa gtagaaacgc cgataccgct ggcgttcacg
     4681 cgcaggttcg tgatgcggag gatgcgggtg ccggtcgtgc cgggggcatc caccgggata
     4741 ccgaggaacg caacagcgtt ctcagccacg cggcgtgcga ggtacgtgtt atttgcacca
     4801 gccggaacca cgcccgggat ggggccgagc tgcctggcac ccacggccgg atcgtcaatc
     4861 aacagcgttg cttcggtcgt gctattgctg tccaatattc tgctggtgat ctgtgtgttg
     4921 aggaagatct gaatgttcac ctgcgggaca gccgccgggt ctgcggccgg cacgccgccg
     4981 gtgcactgca gaacgatgtc accgaccagc tcggtaatgc cttcaccccg gatgatgggc
     5041 gtcacggcgc ctgtcgtagc gctgcaactg aaggctgttt gggcgctggc ggtactcatc
     5101 gagcccaaca ttagagctac gagcgccagc accggtaacc acttgcgaaa atctgccatt
     5161 tccttctcct tgtggaaatc tgaaatcgag tcgaattgat ggaagtgctt caacctgagc
     5221 ctcaatggga gtatcgccgc cgcacagcca gctctctacc gcctgtattc gaacgacgtc
     5281 cactcatata tccgtacaag ctcgatcgtt tttgttttat acaacttttt taaatctgtg
     5341 ctcgcgggat tcacgcataa tgaatgccgc tgatacttgg acgcagtcaa aacattcata
     5401 tcactccatg cgagaacaag tcaagcataa agtggaatcc agcatccgaa attactaatc
     5461 gaccacgagc caagccagtt tgcaattaca tcatgggaag gtaacactag tcaacaggca
     5521 ggacacctga atgcggcaat gttccatcga acggtgaaat cttcgcatcg atagcggtat
     5581 tccgaaggcc gaacggtacg gcgagccggc ctgattgccg gccggcgcgc cgtagcggat
     5641 ccgctcattc ttctacatat cgttgcaatt tcttaaggcg cgatttcagc tcttcggaag
     5701 catccctcat ctggttggtg tcgtaaatca ctctcggtgt cataaatata atcagctcgg
     5761 tgcgcccttt gctgtacgat ttacttccga aggccgctcc gatacccggg atacggtgaa
     5821 gaagaggaaa tccgctgctg gtagccccag cgtcttcctg gatgattccg ccgatggcga
     5881 tcgtgtcccc atcctgcaca gtcacctggg tctgaacggc ccttttggaa aaagcgggcg
     5941 aattaatgcc ctcggtcggc ggcggctcag gcgcgctcac ttcctggttg atgaccagcg
     6001 tcacgatgcc cgacgggttt acccgggcca gaatagccaa ggtcacaccg gtgttccggt
     6061 tcgagatggt ttgtgcgaat agcgaactgc cgccggtctg cacgccggtc gccgcttgag
     6121 cggttaaggt gggcacttcc gatccgacgt tgatcgtggc cggaatgctg tcggtggcga
     6181 tcacgctcgg cgccgacagg atcctcgtga acgtcgcact ctcgcgcgct tccaggaacg
     6241 ccaacagttc acggctctgg ccgacgagag tcccgatcgt gaagttcaag acaccgccgt
     6301 ccagcttcgc ccggaactgg ttcgtgggaa ccgcggcgcc gcccgattca ccccgcctct
     6361 gcagaaacgc cgaaacgccg ctggccagcg cgcctgtcaa cgacacttcg tagattttcg
     6421 cgtccaccag aacctggcgc ggcggaatgt ccaactcgcg gaggatcttc agaatacttt
     6481 catactcctg ttgtgttccc tgtatgagca aggtgttgtc catgggattc ggaacaacgc
     6541 gtggaatccg tccgccggcc attccatagg aagatgcgcc aagatatgtc ccggtctggt
     6601 cggccgagcc ggtggttgca gcaccagctt gagtaccctg gcccgtcact gtccccggta
     6661 gcggcgtgcc tggcgtggcg aaggttgttg gaatcggagc acccatccca tagccgccca
     6721 tgccgccgta gccacccatg ccgtaaccac ccatcccata accgcccatg ccgccgtagc
     6781 cgcccatgcc ataaccaccc atcccatagc cgcccatacc gcccataccc ccgtagccac
     6841 ccatgccgta accacccata ccgccatagc cgcccatgcc gccatagccg cccatgccgt
     6901 aaccacccat gccgtaacca cccatgccgt aaccatatcc gccgtacagc atcatgatgg
     6961 accccgcgag gccctccgca cggccgtact tcacacggta cacatagttg tccacagcgc
     7021 ccgcggtcac cttcaccgcc acatccacgc gtttgagcca tttctcgact tcctcgaaga
     7081 cacctggatt cggcgcgatg gcgataagag tgttgatgcg gtcgagcgga aggaatttga
     7141 tgggtgtgtt tttctcgctg agggaaatgc ccttgaaaat gctttcgagc tcgcccgcaa
     7201 tgtcgctcgg ccttccattc gtcacctcga acaaccggac gcgctggctc gcgaaccgct
     7261 cgctgtcaaa gagtgcgatg agttccatca gccggttgag gttgcgccca ctgtcgagaa
     7321 tcaacaagag attcgccggc ggatacgtca ccgtcgtgga gttctcgccc atgaacggct
     7381 gtagtacttt caccagttca tccaccgtcg cgtacttcag aaaaaccaaa ttcaggtacg
     7441 tccggtcgtt ctccggaatc tgctggatgt ctgtcgtggg ctcaaggggc agccgcgaaa
     7501 tctggtcgag cgggacgatg cggtagatat cgcccacctg caccataccg gcgttgttga
     7561 tacgaagaat taattcgagc aggtttcgcg gatccagatc tctcgtatcg ccgtaagtat
     7621 tcagaaccac cgcccccttc aggcggggat cgagaatgta gttgatcttc aggatgcgcg
     7681 cgagctggtc aatcacctgc gacagtgagg cattctgcaa agagagcccg ccaaagctga
     7741 ccgcgcccgg tgggctgggc tgcggcgtag tggccgtcgg gccttgcggc ttcgcggcag
     7801 cggccggcgg ctgcgggctc tgttgtgttc cggtttgagg ctgtgcctgg ggcggcggtt
     7861 gtttcggttc ttcctgcttt tcggcttgtg ggcgatagat gggaccaaac cccggcggca
     7921 atccacgcgg cggcggtgga atctcttgcg caaaaagcaa cggcagggcc atcagggccg
     7981 cgattcgact ggagagaacc ttcacgcgct actccttcac gcttccggac gccgcttcat
     8041 ccggctggtc cgctgccggc ttcggtgggt agtcccgttc ccaaaccgcc cgctccactc
     8101 cggtcaactc ggatttcttt cttgtccact gcgattttcc gaagggtgtt tggcgagtaa
     8161 agcggacgct gtcgccttct tcggctgcgg tcatttcggg tggcacatcc tgcgtcgtcg
     8221 ccccggactc cggagcgccc tcttccttgc gcacgccgaa cggcgttctg gtgtaagtcc
     8281 agacttttcc gttggaatcc gtgtagaggt agctattcga tccaacctgc ttcgcatcgg
     8341 gagggatagt gagctgaggg gtcgcgcgcg gttcggcttt gcggacgcta gtcgaagccc
     8401 gtggcgcggc tttgcgcgat tccgtcttac gcttcccgga tccttcgcct gcggcgcaga
     8461 caaacggcac actgatcgcc agtaaaatca gaagtctcat aatttgtttt cctgctactt
     8521 gaccggttcc catcggcacg attcaccgaa gggggtcttc gtcacgactt tccgcatgct
     8581 tccctgaacg gtgccggaag gcgatgtatc gcccggctgg catgccttca aatcggcgcc
     8641 gatatccgca cccggaccgg atgccgccgg tcccgcggac accgttgtcg ccgatgtaac
     8701 tttcgacccc gcggcggccg gcggcggcgc ggcctctgcc gtctccggct tgctcttatc
     8761 cattaattca tcgagcgtcc gggtaaccgt ttccccattc cactcgaacg cgatctcgcg
     8821 attgttcgcg gccagcaatt tgaactggcc gatcgcctcg cctacctgat agcctcgctg
     8881 cggccctccg ggtttctcgc tcaggaacac tgtgggaccg ccgccaaaat ccatgacgcc
     8941 gtgcgccacc ggcaacgccg gcatcggagc gggttcggcg gcttcgatga cgacgtttgg
     9001 attcctgtct tttgaaaaca gcatcatctg cgctacttcc gcgtacgacc cggcggttac
     9061 cgggttcacc ggtgcggcgg gagcgatcgg tggcgccggc aaagccttca cggtttgttc
     9121 cagcagtttc gtctctcgct cgcgcgccgc caaccagtcc tgccgcaaac gcgagcccag
     9181 ccataacacc agcgccagca gaacgatatt caacagccac aacttgcttt tcaaaactgc
     9241 ccgggtcctt tcttctccgg caggaggcgc ttcggaacca gacccgacac ggtcaatctc
     9301 accggcacga gcttttcctt gttgctcgtg gcgccgatgc gcaagtccgt ggtggcaatc
     9361 aattccggct gagcagtcag ctcggccaga acgttcacaa cctgctcgat gccgcactcc
     9421 atcgacaccg aaaccgatac ttcgccgtaa tcgtctccga atggcttcgg ctgcgcgaac
     9481 tcgttgcctc gaatgtccac caccggcgtc tgcgcgcgcc cgatgcgccg caggatttgc
     9541 agcaactgcg cctgcgcctg ggctgccgta tcggcctgaa tcaatccctt ttcacgctgt
     9601 gcaagctcgg cggccacttg ctttagcgca tcctgttttc cgggtgttgt ggccgccatc
     9661 tgccgcacgc gtgtcagacg cttttcggct gccggaacag aatcggccac cacgaccaca
     9721 gccgccggag acgaatcgcg ggataaaacc tggaacagga cgatcgcggc gacggccatg
     9781 gccagaatgg ctaacgctcg cttgtcccgt tgcccgatcg tcattcgggc accccctccc
     9841 gcgtcgtacg aatgcgaaac acttcccccg ttccggtgcg ccccaatggc atcgtgaact
     9901 gcgagtcctg aaacaggggc gattcgtcga gcactttcaa caacggtgcg gcctgctccg
     9961 cttcgcccgc gatgtgcacg aacgctcgcg tgacctcgag ctggctcacc cagatgggcg
    10021 gcgccagcac cgtcgtcaat tcgtttacca cgtccatgtc gtgtttcgac cggcggcgaa
    10081 aattatcgag caaggcggtg cgccggcgcg cgagctgtgt gtcgcggtcc agcgccgcca
    10141 cctgccgcgc gtgcggctcg agcttggcga tctcggattc cagcaccgaa agataacgcc
    10201 gttcccgata cttgccgtgc actgccagcg cgcttagcag cagcagaagg caagccccga
    10261 gaaagatcgt aggaacatac atcaatcgcg agcttgtgct tcgcctgccg gcgggcagca
    10321 gattcgccgc gagcgccagg cgcgggcacg cgcccgccag cgccgccgca tacggaagcg
    10381 ctctttcgcc cagcgcgcga cggtactctt cgtcgtccga gggagcgtct ttcggcaacg
    10441 gcagcagttc ccgcaggtgt ccggtctcgc tgtccggttc caggcgcaac tccgcgaggg
    10501 ccagtgtccg cgccctctct tcacttccgc ggacctccgc ggaaaaaacg gcccgcgcct
    10561 cgctttcccc gtaaatctcc accgaaccgt tgaagggccg tattaccgcg aagccatctg
    10621 ccggtggact ggagagcaac cggatggcgg agtagatcgc cgccgccgag aaggtaaacg
    10681 aagccgtctt gatgccggcc tcggcgaaca gcgatgtata gcggtctacg gtctcacgcc
    10741 gcgcaattcc gatcagaatc gaggaactcg aacccaatcg cgcccaggca tagacgacgt
    10801 cgtcctccgg gtaggggtgc tgagaatcca cttgcagcgc aatggcggct tccagatccc
    10861 tgtccggcac cccgggaagg ttcagttgcc ggacaatgat ctccgcacgt ggcagcagga
    10921 ccgccgccgc tatatgtccg gctccaagct tttttgtgaa ggccgcgtat tcggcgcccc
    10981 actccgatgc ggggcgtgca gcatacttct cgattgtggc ggatccgagg atttgggagc
    11041 cagtgggccg cacgcgcgtc acaagcacgt gcaggtcgtc gcccgaaatc tcgatggcga
    11101 cgcccgtacc aaaagccagc cagcgccgga ttttcgtact cactgccatg cccgaaggtc
    11161 cacccatgtg ttgtcgtacc agcgaagcac ggtgtacggc ggctcttttg caaaatctgc
    11221 cggatacttg accaccgccg ccaccgagcg ccgcaaatcg gaaacccggt tttcacctat
    11281 acgtaactgc gctgttgcgc gaatcgtaaa aatcgttccc gcgctcactg ccaatttgcg
    11341 gaaacccggc ccacccgcct cggccagtgg cgccagatcg gcgggggagc gaaacggcag
    11401 cacccgccgc cgctccagaa tggccgcgat cgaattgggc gtcagtccaa tagccgccat
    11461 caaaaccggg gatacggtat tcacgtcaaa gccggcgaga gccccataaa cggaaaggca
    11521 gtcccgaagg ccgccccgcg gaatgaggtt gccttcgggg ccgcgctcgt agcttccata
    11581 aaacaggtcc ggcgtcatac ctttcacaag gagaagttct tcaatctcct gaaaagacgc
    11641 atggcgcgcg cgaaaagacg gagccagcga aagataatac tggtcgaaga gtgtgacggc
    11701 gccggggggc actgccgacc gccagtcgat gataccggcc acgatctcgt ccgcctgccc
    11761 cggcgggata ccggcggcaa gcatcaggcg aagcagttct tcggcgggag cggtattgat
    11821 gttcagcttg gaagtttccg gaatcacctc cacaaaaact tctccggttg gaaatggcat
    11881 ccgcagaaat ggcttatccg gcccgtagta gcgcggagtg ccatcggggt tccggtattg
    11941 aggcccccac tgcatgtaga gcaacgcgcg gtctacagca cccgccgcga gatagtatgc
    12001 gcgcacggaa tccgcgtgcg tcgacgcccg ctccgtttcc ccgcgcacgg agttcgcgac
    12061 ggagaacgcg atggccgaca gggccgccga cagccagagc acagccagca aagccccgcc
    12121 gcgctggaca gaccactccc tcacggtcgt ggctcggaaa cgaatactga gccgcgagcg
    12181 taagcgagcg gttcccgggg cgctagttct cataagtcac catcggatcc ttggttaccc
    12241 ggatgggagc ggtcagcgaa atcagttgaa ctcgcgacgg gtcgggacgc agcggtgcca
    12301 tctccacgcg aaccgccatg ggcaggtccg cccgcaccca tcgcggagtc caccgctcga
    12361 actccggcgg cggcgcgggc tcacgataca taaaccggca gaacgccaat ttatccgcca
    12421 gcacgaacga ccgcggaccc acctggaccg gtggaaaccg cggcacggga gcgcccgtga
    12481 tcggatccgg tcccaggcca aagcagaacg gcgcggtgga aagcgatccc gcaaacaaat
    12541 gttcgttgac caccaggcga acgccttccc ggttctcgcc cggaataacc tgaaattcca
    12601 gcagacgcgg cattcctctc gcggcttcct gcagcgaata ggaagagacg aatcgcatgg
    12661 attcgatgtc gccctggaag aagctcatgc gcgtgggagg cctgtccggc gtctccacgc
    12721 aatcgccttg cactgccatg aagccggcga tctgctgctc cagaatctgt tgcgcgccgg
    12781 cgacacggcg gttctccatg agcctggcgt tcgacttctc catcgcgccg aatcctacac
    12841 gaatcgccat gaggatgccg agcgaaacca ggccgaccag gctcaccgcg atcagcagtt
    12901 cgagcagtgt gacaccgcgg gacgcccgct tcactggacc acgcctccgg ctacaatatc
    12961 ttcacgtgtc agggtgccgc ggcggtagcc ctccagcgta aacgtgcgcc gctgctcgcc
    13021 ggccgtccac cagacctcca attccacgcg ttccagaatc ggcgtgccgg ggccggcttt
    13081 cggcggcatt tcgaaaggcg tcacacgcgc gcgccatccc gatttcattc cttcgggggc
    13141 ttccaacgcg ccttcgatca ccaccagcct gggaagcctg gactgaacca gaagctcgtc
    13201 catcttcgtc cgggccagaa gcgcggcacg atcgtaatcg atcaggcgcg agccgctgcg
    13261 catggaggtg gacaggctgc tcagcaggcc ggcgacggca accgccatga tgaccgttgc
    13321 caccagcacc tcgagcaatg tgaaaccgcg ttgattcact gctgttctac ccgctccacc
    13381 tgcggtacgc cggtcgccgg atccacccgc acgatacgcc gcgcgccccg ccggttcaca
    13441 atctccacgc cgaagcgcgg tactgtgccg cccgggtaga gcatgaaccg gcgcgcatct
    13501 tcggattgat gcggggattc atacggcaag ggaggcagca cggcggcgat ctccactcca
    13561 tcgggcaatg tcatttcgcg caggaagccc ggctcactgg accgcaatct cagaaggttc
    13621 tcacccggcg caatcgttac ctccatcacc tgctgcctgc gctgcgcgcg attcaaaccg
    13681 ctattgaaaa aactgacgat ggagttggta gcggacgaca gccgcagcga atccaagccg
    13741 gagttcaccg aaggaaaagt gatgccggcg agcagcgcca gaagcgcggc gacgaccatc
    13801 atttccatca gcgtcacacc gcgctcggaa ccactccctg acggtcgcgg ctcagaaacg
    13861 gagccgtgcg cgtcagcgag cggtctgatg gcccacgacc gtaaacaggc ggaatacggc
    13921 ttcactgagt tttccaactg acaacgtcgc cgttgatccc ttcacccccg ggctggccgt
    13981 cggctccgta cgagatgaca tccggctcgt ctccatgttc gccgggatag acgtacacat
    14041 acggacggcc ccacgggtcc accggtatct cggccggcag atacggcccc tgccactggt
    14101 tgacgttctg cgggcgcgtg cgcagcgcct gcaagccctg ctcggtggtg ggaaaggttc
    14161 cggtgtcgag cttatacgtg ccgatcgccg tcatgaatga attaatctgc gtgcgggccg
    14221 cggtaatccg cgcggtgtcg gtcttctgca acatgcgtgg cgcaaccacg gcggcaaaca
    14281 gcgcgatgat cgtcaccacc accagcattt caatcagcgt cacaccagcc tggcgccgtt
    14341 cggttcgttt tttcattgga agctccattt ccacggcctc gtcagatggc cacttcgttg
    14401 atgctcgtga tggcgagcag catgctgagg atgagcgcgc ccaccatgat acccataacc
    14461 agaatgatca acggctcaaa cagcgccgtg aaccgtttga tggcagcacg cgtgtcggcg
    14521 tcgtagatgt cggccatgcg gctgaacatc tgatgcaact ggccggtctc ttcgccgacg
    14581 ctcagcaggt gtcccgccag cggcggaaac tgggccgttt tcttcagcgg tccggcgatg
    14641 ccctccccgc gcttcactcc ttgcgccacc tgcgctagag agccggcaat cagactgttg
    14701 ttcagaatcg cgctggcgat gctcagcgat tgcaccaggg gaacgctgtt ggcgatgagc
    14761 gtcgacatcg cccgcgcaaa ccgcgcggtc tcagccttgc gcagcgcgtc tccgagcagc
    14821 gggattcgca gccggaagcg gtcccaccac agccttccgc cggatgtgcg gacgtaggcg
    14881 ctccaggcca gtattactcc taccatcgag gcaataccga tccagccgta cgcctgaacg
    14941 aaccggctgg tctccagcat gatcttcgtg ggcagcggca tttcgattcg cgattcctcg
    15001 aacaccgaag caaagcgcgg cacaacaaaa tacatcagca ccagaatgga ggccagcccg
    15061 accactgcca atagcgccgg gtacaccatg gaggagacga tgtagttgcg caggtcgtca
    15121 cgcgttcgtt cgaagtccga gagcttttca aacactaccc ccagcgagcc gctcgcctcg
    15181 cccgcccgca ccatgttgac gtacagttgg gagaaatggg ccgggtgggt tgacaggctg
    15241 tcggccagcg atttgccgct cttcaacacc cgcagaatgt ccagcacgat aaagcggaat
    15301 tgaggccgct cggtaagctc gctggtgatg gaaagcgcgc ggtccaacgg cacaccggcg
    15361 ttgagaagcg tcgaaagctc ctgcgtgaag aacagaacat cgcgccgctt gccgcggttc
    15421 atcgccggca gcttcagttc gaacgatttt ttctgctgga ctcctacata gacgggcgtg
    15481 agcccctgct tgcgcagttc cgaagcggcc agcttttcgg tatccgccgt cagagtgccg
    15541 ctgcgaactt ttccatccgc tgcgaccgcc ctgaaatgga agctcgccat taaaactcct
    15601 gcgtcacccg catgacttcg tccgccgtgc tcaacccgtt gccgaccttc agccagccgt
    15661 cctcgcggag ggtgcgcatt ccattacgcc gcgcggccgc cgtaatatca accgcgtcgg
    15721 cgttgttcat gatgagccgc cggatctcgt cgttcaattc catcatctcg aagatgccca
    15781 cacggccggt gtagcccgta ccgaaacagc tatcgcatcc cgcgccgcga taagtgtcca
    15841 ccaggttgcc gtcgggtgtc atcctctgcc cggcgggctg cttgcaggcg gggcagatga
    15901 cgcgcacaag ccgctgcgcc agcacagcta ctaaggacga agtaatcagg tagttttcca
    15961 cgcccatgtc ggtcagtcgc gtgatggcgc tcggtgcgtc gttcgtgtgg agcgtagaaa
    16021 aaacaaggtg gcccgtaaga gccgaacgaa ttgcgatgtc ggccgtttcg cgatcgcgta
    16081 tttcgcccac catgatgacg tcggggtcct ggcgcacgat gtggcgcagg ccggaagcga
    16141 acgttaaccc gatttgggga ttcacatgga tctggttgat gccgtccatc tggtattcca
    16201 ccgggtcttc gatggtgatg atcttcttgt cgggcaggtt gatgcgcttc agggcggaat
    16261 aaagcgtggt cgatttgccg ctgcccgtag ggccggtcac cagaaagata ccgtgcggta
    16321 aggacgtgaa gtgctccatg cgcgcgagca tcgcgtcgtc gaagccgaga ctccgcagat
    16381 cgtaaaaatc gcccgccgag cggtcgagca accgcatcac aacactttcg ccgtacagcg
    16441 tcggcagggt ggatacgcgg aggtcgatct cgcgccccaa cgtcttggtc ttgatccggc
    16501 cgtcctgcgg gagccggcgc tcagcgatat tgagtttcgc catcagcttc acgcgggaaa
    16561 tgatggctgc cttcaactcg cgcggcggcg ggtcctgttc gtacagcacg ccgtccacgc
    16621 gataccgtat gcggaactgc ttctcgaacg gctcgacgtg gatgtcgctg gcacgcttct
    16681 ccacagcttg ggcaatcatg gcgttcacca gccggatgac aggcgcctcg ctcgccatgt
    16741 cgcgcaactg ttccagatct tcggacgctt gcccgccgtc ctcgaattcg gcctccgccg
    16801 ccttctccgc ctggccgtag tacttgtcga tcgcgtcgag gatctcctgc tccggagcga
    16861 gcgcgggctc gactttcagg cccgtcaccg ccgtgaccgt ggagattgtt tccaggtcca
    16921 gcggatcggc catcgccagc gacaccgtgg agtcaaacaa cgctaccgga aggcaacgga
    16981 actggcgcaa aaaacgcggg gacaagcctt cggtttccgg tgttaccgga ggaggcccat
    17041 caatcgcgac caggggaagg gataactgat cggacaatgc cgccagcaca tcgcgctggg
    17101 cgatgaagcc cagatcgaca agaattttcc cgattttctc gccgcgttct ttttggattt
    17161 cgagagcccg ctccagctca tcgccggtga tgagctttcg ttcgatcagt acctctccca
    17221 ggcgcatcgt cagtattccc ggactttgag agcggcatcg agagcaggcc gggcaatccc
    17281 ccccgccttg tattcgacca gcgttctgcg cggtgcctgc atcaaaatct gcgaggggag
    17341 cccttgcctt ctctcccact cccagtaaaa gatcgtaacg gcaaccacaa tctgctgatc
    17401 gagcggtacg gtgtccagag cggccgccgt atcgacaagc atgtcgcgca tgacatcgcg
    17461 gagccggtct acgcgctcca gcttccgtac gcggagttgt tccttttgcg gatctgtgta
    17521 tctcggacga aacggagtga gcgaagcgct cggcagcaga tcgacctcgg cggtgaggac
    17581 tacaccatag ccgtccaggt agacgccgcg cgaggaaccg atcaggcgga tgggattatc
    17641 gatgtcgaac ttttcgatgc ggccgttgaa gtttgcttct acaggctgta tggcggtata
    17701 gcccacgcgc accgtatcct gcgcggagag aaccggcagc gcgagacaca atagcagagc
    17761 ggcatatctc atcgcgacgg caccatttcg ctgctcgcca gccgtggata ttgcaggatg
    17821 tcaacctgct tcttcagaag cgtaaacgcc tgttcgaacg tcaccagatc cgctcggcgt
    17881 tcgaagttca ttttgctttc ggaggcagcc accagttttg ccatctccgt acgcagccgt
    17941 gcgtcgtgct ccgctacggc ctgagcgaca gcagccggta tgcgtctgga cacctcggct
    18001 tcgatgcgag cctcgaccgc ttcctggttg taggcggcgg tctcaggcac agcgggcgcc
    18061 ggacgggtca gcccatgcac cagaatcgcc gccgccagca ccatcgccga gacgaaaccg
    18121 aggcgcggcg ccgagttcca taggcgctgc caccagcccg gctcgaacac ccgatccgaa
    18181 acaaaagcga tccgccgcgg catttccacg tccggcacgg acgcaagcgc cgcctgagcc
    18241 gttcgcagcc tctccagttc ttcactgcat gcaggacatg cacgtacatg cgcttcaacc
    18301 gtgaggcaat ccggcccggg caattcgccg agcagatagt ccttcaaaac atcaggcgag
    18361 caattcattc cacaccccgc gctttcactg gagccaaagc gtctttcaga aggtcgagcg
    18421 ccgtgtacaa ccgcgacttc accgtcgaaa ccggacacga aagaatttcc gacatctcct
    18481 cgaatttgaa tccctggtag atcttgagca ctaccgcttc ccgctggtcc gccgacaacg
    18541 tcgccatggc ccgctcgatg gcgatactca cttcgatggg cagcattcgc ggattgtgcg
    18601 cgggcgccgg cggttccttc agctcggttt ccgggcgatt cttccttagc agggaatacg
    18661 attcgttgtg cgcgatccgg aacagccacg gcccgaaccg ggcagggtct tccagcttcc
    18721 cgagattctg atatgccttc agaaaaacat cctgcgtcac gtccaacccg tcttcccgat
    18781 gcccgaccag ccggtagatg taattgaaga cacgtttttc ccatcgggaa acaaggatgt
    18841 tgtaggcgtc cacgttcccc ttgcgagcct tgtgaatgag gtcgcggtct tcaacctccc
    18901 gaaagatcaa atcttcggct ccaatgcgta gagacgcggc tggtgatgaa aaagtcggag
    18961 atggaagatc atgcggtcag gtatcgcgca aactgcagcg tgaccggttt gttcaaccgt
    19021 tcgaaatccc ggtacttcat gtacacgacg tcgcggtgtg taccggcgtt gaaggcgatc
    19081 tcctcttcct cagccattga gccatccaca tagagaggaa gcccaaaaag actgcccatg
    19141 ggcggcatag cgcccagttc gcattccgga aacagttcat cgagctcttt ctcggtggca
    19201 agacggatgt tcgccacccc aagcgccgcg cgcaactctt gcagatttac ccaactgtcc
    19261 gccggcagaa ccgccattcc gtagccggtg tcgctaaaaa aaacaatgca tttggccact
    19321 ctatgggcag cgactttttc ggccgatgcc acctctttgg ctgtaaaagc gagcggatga
    19381 acgtggtggg tgtatccggc tccgcttcgg tcgaggaagt cctggagcct tttcaacatc
    19441 gccatcggaa gcctcctttt cggcagcttg cctgccggtt acacggagga agtccacagt
    19501 cgtttccttc aggataagac aaaatagata aaagcaatca ggcaggatta ccttgaggag
    19561 ggtgccatat tgatttcgac actactgttc ttgctgctgc tttcacaggg cattccgaac
    19621 gaacagcgcc tgaaccagca ccgggtcacg tcgcggaaca gccccgcgcc ggaaccgggc
    19681 gccgagaaca agaagatccc ctcgctggtg gagggcttgc gctccgccca tgactggtac
    19741 acgaagaaac gccccgagct ggtcaaactc tggacgacga tccttggcaa aaccgcgccc
    19801 gcgccggaag acaaacagtg gttcggagat atcaccaaag ccgtcgttca cgacgagcag
    19861 gataaaggca gctacacgcg catccgtctc gacctgccaa tggaaaaaga cttctatcag
    19921 caacacctcc tgctgctgcc ga
//
LOCUS       DASTMK010000046         4853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_290779, whole genome shotgun sequence.
ACCESSION   DASTMK010000046 DASTMK010000000
VERSION     DASTMK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290779"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFQ79_01995"
     CDS             <1..314
                     /locus_tag="VFQ79_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138446.1"
                     /translation="EWLKANLYNVSGEENPKWAFLPRGTEGSPAPPPVTPPPPDPVVS
                     SCDINKDNSVNALDVQLGIDQALGTSSCTGDLDSNGRCDVIDVQRIINAALGGVCRTG
                     S"
     gene            386..1474
                     /locus_tag="VFQ79_02000"
     CDS             386..1474
                     /locus_tag="VFQ79_02000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0138447.1"
                     /translation="MSTTSPAISVVVPLYNKERYILRCLESIRRQTFSDYEVIVVDDG
                     SSDGGGSLATAFPDPRFRVVRQQNRGEGAARNRGISEAKGALIAFLDGDDAWEPGFLD
                     AVAGLSRQFPQAGILATGLRRVDGEAYDLETTFKAAGAGRTSLIKKYFHYAKQGNFVS
                     SSSVAIPRFVFEQVGVFLEGEPIGADRDMWVRIALRYPIAYDSRILAIYYTDAAGRSF
                     PRWKSNPPYPPAARTLRRLLDTGVLPPAKRRDAVFLLNWLLLRHAYWLLDLGDRDTLM
                     RHLKEESFSLWTYRWRALLLRSATAIVPVRLLSAVKCRAVLLGTRIRRTLGSLFSAGR
                     YEWWFGRRVVSRQVPAHSPVRAVAAQSF"
     gene            1478..2959
                     /locus_tag="VFQ79_02005"
     CDS             1478..2959
                     /locus_tag="VFQ79_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU0138448.1"
                     /translation="MKISWLFQQSALYFIGNIVRRGVGFLMIPVYTKFLTPADYGLIE
                     MIELFVSVSVIILGVLAMNDAMVRIYYDYPDERSRGTLVTTALVGITSLSLLLAGAAF
                     IGAAPLAVLMFGSSSSAWLVRLAFLAMVLGNITELALVYSRIKQRSLFFVLFSATQLA
                     LTLALNIWFIAYRGMGVWGFVLSKLICTGLGAAILVVLLLRETGFGFDWEQARRMAHF
                     GGPLVITSACFFVIHFADRFFLNHFVNLGEVGVYALAYKAGFLVTYIVGEPFARVWAV
                     SLYAHTSTEGWRHQFGRIARYFVLALFLVAVGISVVADEAVPLVAAPAYAQAAMLIPF
                     IAFAYVFREIGDFFRDLLFINKRSGLFSRITIVCALLNIGLDYWLIQKYQMKGAALAT
                     LLTWLAYMAGCWVAAQYEHHIAFAFRSVALLSVLSAVVLFTSTLIGEVAMLGRIGIDV
                     LLVLGFALVAWLIGYFPAEEKAMMRSFLSQRRRLLVKAVSHAS"
     gene            2988..4061
                     /locus_tag="VFQ79_02010"
     CDS             2988..4061
                     /locus_tag="VFQ79_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0138449.1"
                     /translation="MKRLLYRSGLFSLYHRVRNRRSLTVAMFHRVLPENDPRWPDADL
                     NWTVTDRLFRNCLEFFRKHYNVIGIDDLLCAGDGGESLPARPLLITFDDGWADNEQYA
                     AGILQEYRLPAVVFVVAGAVDQEGFWRERLYRQWRRGSIDAGQLDGLWRALPGSADAK
                     PQDWTSAAAFWALANRLDTLEEVSRTALLNTIFQPSSALEGPVMLSAAQLGALVKARI
                     GIGSHGLTHTPIPLAANPQKELKDSRRRLVALLGLEERRVPLALSFPHGLYNEETVQL
                     ARAAGYRLLFTSDPHLASADTVNWHTTLIGRVNIPSRNITDRKGNFRPELLALWLFNR
                     SRVFTDARRKENARISSVASARY"
     gene            4071..>4853
                     /locus_tag="VFQ79_02015"
     CDS             4071..>4853
                     /locus_tag="VFQ79_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HEU0138450.1"
                     /translation="MRDLVFTSVFILILLAAIGRPWIGVLLCAWLGYMSPQRLCYGFA
                     SYFPFFAVAAAVTIASIFISSERKRFPWARETFIFLLFVLWMNISTLFALNPQGAWPK
                     WDQTMKTMLMIFLGMLVMAARSRLHLLVWVIVLSIEFYGVKGGIFAILTGAQYTVQGP
                     PHTMLENNNALAIFMAATLPLMRYLQLNTESRLIRQGLGVGMGLTAVAVLSSYSRGGF
                     IALCTVGFLLLIKSRRKWLLAGAMIVVIPLMLSLMPQQWFDRM"
BASE COUNT          840 a         1437 c         1434 g         1142 t
ORIGIN      
        1 gggagtggct gaaagcgaac ctctataacg tgagcggcga ggaaaatccg aaatgggctt
       61 tcctgcccag gggcactgag ggttctcccg cgccgcctcc cgttacgcct cctccgccgg
      121 atcctgtagt gagcagttgc gacatcaaca aggacaattc cgttaacgcg ctggacgtgc
      181 agttgggaat cgaccaggcg ctcgggacca gctcctgcac gggcgatctc gactccaacg
      241 gacgctgcga cgtcatcgac gtgcagcgta tcattaacgc cgccctgggc ggcgtctgcc
      301 gtaccggctc ttaagtttgt aagttaaacc cgactctcaa tccatggcga gggccctcgc
      361 gagggggctc tcgcaccgct cgcccgtgag tacaacatcc ccggccattt ccgttgtagt
      421 gccgctctac aacaaggaac gctacattct ccgctgcctc gagtccattc gaagacagac
      481 gttttcggat tacgaagtca tcgtggtcga cgacggctca tcggatgggg gcggctccct
      541 ggcgacggct tttcccgatc cgcgattccg tgtcgtaagg cagcaaaacc gcggcgaggg
      601 cgccgcccgc aaccgcggga tctcggaggc aaagggcgcc ctgatcgcgt ttctggacgg
      661 agacgacgca tgggagcccg gcttcctgga cgcagtggcc ggactgtccc gccagtttcc
      721 acaggccggc attctggcta cgggcctgcg gcgcgtcgac ggtgaagcat acgatctcga
      781 aaccacgttc aaagccgccg gcgccggccg caccagcctg atcaagaagt actttcatta
      841 cgccaaacag gggaacttcg tgagctcctc gagtgtggcc attccacgct tcgttttcga
      901 gcaggtagga gtattcctcg aaggcgagcc gatcggcgcg gaccgcgaca tgtgggtgcg
      961 catcgctttg cggtatccca tcgcgtacga ttcgcgtatt ctggcgatct actataccga
     1021 tgcggccggc cggtcgttcc cacgctggaa atcgaatccg ccttatccgc ccgccgcacg
     1081 cacgctgcgc cgcttgctcg acacgggagt actgccgcct gccaaacgcc gcgatgccgt
     1141 cttcctgctg aactggctgc tcctccggca tgcgtactgg ctgctcgatc tcggagaccg
     1201 cgatactctg atgcggcatc tgaaagaaga atccttctcc ctgtggactt atcgctggag
     1261 ggcgctcctg cttcgctcgg ctacggccat cgtaccggta aggttgcttt cggctgtgaa
     1321 atgcagggcc gtgctgttgg gaacccggat cagaagaaca cttggatctc tgttctcggc
     1381 cggccggtac gagtggtggt tcggccgccg cgtcgtatcc agacaggttc ccgcacactc
     1441 ccccgtccgc gccgtcgctg cccaatcgtt ctaactcatg aagatatcct ggctgtttca
     1501 gcaatcggct ctttacttca tcggaaacat cgtacgccgc ggcgtcggct tcctgatgat
     1561 tccggtctac accaaatttc tgactccggc cgattatggc ctgatcgaga tgatcgagct
     1621 gttcgtttcc gtctcggtga tcattctcgg cgttctcgcg atgaatgacg cgatggtgcg
     1681 gatatactac gactatcccg acgagcgtag ccgcggcacg ctggttacga ccgccctggt
     1741 gggaatcact tctttgagcc tgctgctggc gggtgccgca tttataggcg ctgctccgct
     1801 ggcggtcctc atgttcggct ccagcagcag cgcgtggctg gtgcggctcg cctttctggc
     1861 gatggtgctc ggcaatatta ccgagctggc gctggtctat tcacgaatca aacagcggtc
     1921 tttgtttttc gttctctttt ccgccacaca gcttgccctc acgctcgctt tgaacatctg
     1981 gtttattgcg taccggggaa tgggagtctg gggatttgtc ctcagcaaac tgatctgtac
     2041 gggactgggc gccgcgattc tcgtcgtgct cctgctaagg gaaaccggct tcggtttcga
     2101 ctgggagcag gcgcggagaa tggcccactt tggtggaccg ctggtgatta ccagcgcctg
     2161 cttctttgtg attcattttg ccgatcgctt cttcctgaat cacttcgtca acctgggaga
     2221 agtcggcgtg tatgcgctcg cctataaagc aggctttctg gtcacctata ttgttgggga
     2281 accgttcgcc agggtctggg cagtgagctt gtatgcgcac acgtcgaccg aaggatggcg
     2341 gcatcagttt ggcagaatcg cccggtattt cgttctggcg ctgttcctgg tcgcggttgg
     2401 catcagcgta gtggccgatg aagcggtccc cctagtcgcc gccccggctt atgcccaggc
     2461 ggcgatgctg attccgttta tcgcgtttgc ctacgtattc cgcgagatcg gcgatttctt
     2521 ccgcgacctg ctgttcatca acaaacggtc ggggctgttc agccggatca ccatcgtttg
     2581 cgctctgctc aatatcgggc tggattactg gctgatccag aagtatcaga tgaagggcgc
     2641 cgcgctggca acgctgctga cctggctcgc ctatatggca ggctgctggg tggctgcaca
     2701 gtacgaacac catatcgcgt tcgccttccg gtccgtggcc ttgctgagcg ttctctccgc
     2761 cgtggtgttg ttcacgtcaa cgctcatcgg tgaagtggcg atgctcgggc ggatcggcat
     2821 tgatgttctg ctggtgctcg ggttcgccct ggtggcatgg ctgatcggat attttccggc
     2881 agaggaaaag gcgatgatga ggagctttct gtcacagcgg cggcggctcc tcgtaaaggc
     2941 tgtatctcat gcctcctgaa cgcaaacccc ggtttgtttc gagtatcgtg aaacggctcc
     3001 tgtacaggtc cgggctgttc agcctgtacc atcgggtgag gaaccggcgt tcgctgacgg
     3061 ttgccatgtt tcaccgggtc ctgccggaga acgatccccg ctggccggat gccgatctga
     3121 actggaccgt taccgaccgc cttttccgga attgcctgga gttcttccgc aagcactaca
     3181 acgtcatcgg aatcgatgat ttactatgcg ccggcgatgg cggggagagc ttgcccgccc
     3241 ggccgctgtt gatcacgttc gacgatggat gggcggataa cgaacagtac gccgccggta
     3301 ttttacagga gtacagactg cctgccgtgg tgtttgtcgt ggccggcgca gtagaccagg
     3361 aaggtttctg gcgggaacgc ctgtaccggc agtggcggag aggctccatc gatgcgggcc
     3421 agctcgacgg tttatggcgt gcgctgccgg gttcggcgga cgccaagccg caggattgga
     3481 cttcagcggc ggctttctgg gctttggcca accggctcga caccctcgag gaagtatcac
     3541 ggacggcctt attgaatacg attttccaac cgtcctccgc gctcgaaggg cccgtgatgt
     3601 tgtcagccgc tcaactgggc gctcttgtga aagctcgcat cggcatcggt tcccatggcc
     3661 tgacacacac gcccattcct ctggcggcga accctcaaaa ggaattgaaa gattcccgcc
     3721 gccggctcgt agctttgctg ggtctcgagg agcggcgggt gcccctggct ttgtctttcc
     3781 cgcatggtct ctacaacgaa gagactgtac agcttgcacg tgcggccggt taccgtttgc
     3841 tcttcaccag cgatccgcac ctcgcctccg cggacacggt aaactggcac accacactca
     3901 ttggccgggt gaatatcccg tccagaaata tcacggaccg gaaaggaaat ttccgcccgg
     3961 agttgcttgc gttatggctg ttcaaccggt ctcgagtatt cactgatgcc aggcggaaag
     4021 aaaatgcccg tatatcatcc gttgcctccg cccgatactg atttcccgac atgcgcgatc
     4081 tggtttttac ttcggttttt atcttgatcc tgctggctgc aatcggccgg ccctggatcg
     4141 gtgtgctcct gtgcgcctgg ttaggttaca tgagcccgca acgcctctgt tacgggtttg
     4201 cctcgtactt ccctttcttc gccgtggccg cggcggtcac gattgcaagc atatttattt
     4261 cctccgagcg caagcggttc ccctgggcgc gggagacgtt cattttcctg ctgttcgttc
     4321 tgtggatgaa catcagcact cttttcgctt tgaacccgca aggcgcatgg cccaagtggg
     4381 atcagaccat gaagaccatg ctgatgattt tcctcggcat gctggtaatg gccgccaggt
     4441 cgcgccttca cttgctggtc tgggtaatcg tgctttccat cgagttttac ggcgtcaagg
     4501 gaggaatctt cgccatcctc acgggcgccc agtatacggt gcaagggcct ccgcacacga
     4561 tgctcgaaaa caataatgcg ctggcgattt tcatggccgc aacactgccg ttgatgcgtt
     4621 atctgcagtt gaatacggaa agccggctca ttcgccaggg cctcggggtg gggatgggac
     4681 taaccgcagt tgccgtcctc tcctcttatt caagaggcgg attcatcgcg ctttgcacgg
     4741 tcggtttcct actgctgatc aagagccggc gtaagtggct gctggcggga gcgatgatcg
     4801 tcgttattcc cctgatgttg tcgctgatgc cccagcaatg gttcgaccgg atg
//
LOCUS       DASTMK010000047        16887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_450587, whole genome shotgun sequence.
ACCESSION   DASTMK010000047 DASTMK010000000
VERSION     DASTMK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16887
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450587"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VFQ79_02020"
     CDS             complement(<1..1008)
                     /locus_tag="VFQ79_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138451.1"
                     /translation="MHLPLQKAARVVALVFSLSILALAQQEPSEVKPAPVDPLGRSTP
                     RGAILGFLEAAQGRDYDRASQYLEYLPGVGAAQRRETARQLQAVMDLHLSTRLNEVSN
                     EPDGSIETELSTNQERAGMIEMPDRKVPVVLKRVSENGIRVWKISNTTVQQAPALYQG
                     LTFPEFERLLPKPLVTVRLLNLPLWQWLALLAILPIAYGVAWLLSWLVRSLLRPLLFR
                     TSTGADDELLRLATGPLRLLLAIIVFHFLRLPLGFPVLMRQFLGTVEVLLSILAVTWF
                     ALRLIDLAAAQIRERMVQTQRLGAIAIIPLGRRVVKVVAISIAILAMLDNVGFNLTAV
                     VA"
     gene            complement(1075..2634)
                     /gene="pruA"
                     /locus_tag="VFQ79_02025"
     CDS             complement(1075..2634)
                     /gene="pruA"
                     /locus_tag="VFQ79_02025"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEU0138452.1"
                     /translation="MSEVPGDFKNEAYTDFPVPENRKAMEAALAAVRAQFGREYELLI
                     AGSRLKTEEKFRSLNPSRPSEVIGIHQKATPELAGMAVDAAYAFFPEWSGTPAGRRVE
                     MLLAAADLLRRRKLEFDAWLVFEAGKTWPEAEADVAEAIDFCEYYARQMLRFAAPAPV
                     VQLPGETGRMVYLPLGVGIIIPPWNFPLAILVGMTTAALVAGNTVVIKPSSDTPTIAA
                     KFAGLLLEAGFPDRSFCFLTGSGAAVGDVLVEHPKTRFVSFTGSRDVGLRINELAAKP
                     QKGQVWIKRVVAEMGGKDAIIVDEEGDLDSAVDGVLASAFGYQGQKCSACSRAIVSEK
                     LYDEFLERLTAKVEKLKVGPAEEPANYMGPVINQRAKESILSYIEKGKQEGRLVAGGE
                     SPPGEGYFIRPTVIADVESKATIFQEEIFGPVLAVTRAKDFDHALELANDSEYGLTGA
                     VYTRNPEKIQKARDRFFVGNLYINRKCTGAMVGAHPFGGFNMSGTDSKAGGPDYLLQF
                     LQAKSIAEKQI"
     gene            complement(2649..2945)
                     /locus_tag="VFQ79_02030"
     CDS             complement(2649..2945)
                     /locus_tag="VFQ79_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU0138453.1"
                     /translation="MAEEMNNTSGRVLWFIAGAAVGAALALLYAPAAGGETRRRISQK
                     AGEGRSAIAESGREILDRGRELYEKGRHIADEAADLFERGRKLVEGEPAPQENT"
     gene            complement(2993..3622)
                     /locus_tag="VFQ79_02035"
     CDS             complement(2993..3622)
                     /locus_tag="VFQ79_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0138454.1"
                     /translation="MPEACTCGAIPPADARFCHKCGRPLFSEPVPEPEEEPVRAEIVE
                     PERPVAAKPEISFHNTAAVRVGFLAAGIASLLITLPTPAFFPLLWLTASGVLAVWLYR
                     RRTGESLTVRAGVRMGWITGVFCFAIATVFFTISVLTIARGGGLAAFYREQLSARGAG
                     EANIEQFIEVLQNPLGLGTVLLLSLIFVFVFFTLLPTLGGALGAKLLKD"
     gene            3704..4672
                     /locus_tag="VFQ79_02040"
     CDS             3704..4672
                     /locus_tag="VFQ79_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0138455.1"
                     /translation="MRPITLLVLANPAARYLAALERLPDSTHIVVGDTKEAFLGKVAG
                     AEVIFTGMGFAPLLRELWPDAARVRWVHSFSAGLDNVLFPELIESTAVMTNARGVFAR
                     SLGEFVIASVLFFAKELRRMIWQQREGVWEPFDVEEVHGQTMGIVGYGEIGRAAAERA
                     HALGMKTIALRRRPELSSDDPLVDRTVGPDGLFDLLREADYVVVSAALTPSTRGMIGE
                     AELNAMKRSAVLINVGRGPVVVESALVRALRENRIKGAALDVFDEEPLPVGHPFYDLE
                     NVLLSPHSADHTATWLDEAMQLFVENFERFVKGEPLHNVVDKSEGY"
     gene            4674..5960
                     /locus_tag="VFQ79_02045"
     CDS             4674..5960
                     /locus_tag="VFQ79_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0138456.1"
                     /translation="MKNRVLLFLLIAATAFAQSYKPAPENLEARKWFQDAKFGMFIHW
                     GVYSVLGDGEWVMNNQKIPIPEYEKLPPKFNPTKFDAAEWVSLAKNAGMKYITITSKH
                     HDGFAMWATKQSKWNIVDATPYGKDPLKMLADECRRQGIKLFFYHSQLDWHHPDYYPL
                     GRTGHASGRPAGGDFNRYLDYMDRQLRELLTGYGEIGGIWFDGWWDKPDADWRLDKTY
                     SLIHSLQPAALVGSNHHRKPFDGEDFQMFEKDLPGQNTTGFSGESEIGNLPLETCDTI
                     NGAWGYNKNDKRFKSSQQLIQYFVKAAGHNANFLLNVGPTPEGTIQPEFVERLQAIGE
                     WMRKNGETIYGTRGGPVAPRPWGAMTQKDNRIFVHVLDWNDELLALPPMPKIKSATLF
                     ATGATVKIQAVDGGLLLRLPKTGRDPVDTIVVLEKQ"
     gene            5957..6913
                     /locus_tag="VFQ79_02050"
     CDS             5957..6913
                     /locus_tag="VFQ79_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU0138457.1"
                     /translation="MNNLPVTVDEIRAAAERIRGVAYRTPVMTSRSFDSVAGIHAFFK
                     CENLQRGGAFKIRGAANFIFSIPKADLSRGVVAFSSGNHAQAVAIAAGVVNTPATVVM
                     PSYAPQSKLAATRGYGAKVIEYDQSSEPREAIAARIAEKSGATVVPPFDHPWTIAGQG
                     TAALELLADYPDLDALLVPVGGGGLISGCAIAAKSTRPDIRIFGIEPAAANDAFLSKR
                     AGRRVRIERTDTIADGLRSPEPGELTFPVIEQLVEDIVLVSEDEIKSAVKFLLARLKI
                     LVEPSGAVGAAALLSGKLPPDVRTAGVILSGGNIDLEQLARF"
     gene            7140..10547
                     /locus_tag="VFQ79_02055"
     CDS             7140..10547
                     /locus_tag="VFQ79_02055"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138458.1"
                     /translation="MPVSLRAQDARGSIVGRVADSTDAVIAGAEVRATNVATGVAAVS
                     KTNEAGNYVIAYLLPGRYTVEVEFAGFKKFVRQGIEVRVGDTVNIEAVLEIGNQAETV
                     VVTAETPLLETADASLGQVVDERRIAELPSFGGAPYNLTLMAPGTINSTNLRQRYVGT
                     PGAQGDFAVDGAGMRNNEFTIDGVANSLDKGIVFVPPQMSVSEFKVQSVSYDASIGHT
                     AGALVNVSLKSGTNELHGEAHWFVRNRIFDTPTLFQNRVGQEVPPYQDHRYGVSGGGP
                     VKIPKLYNGTNRTFWFYLWEANKVQYAYDFQNTVPTAEMKRGDLSPLLALGANYQVYD
                     PATTVFSGGRFTRQPFPNNIIPANRLDPTAQKIMEYWPAPNQTGTREFRNNHFVSYAG
                     PFPVWTHLGRFDHSFNPNHRLYARVMREGFWSASNKRFLSDYDGMQYNQDKWGFALDD
                     VYVFNPEFFMNLRYGFTYRWGASYKFSQGFDLATLGFSPNLISQLPDKSLATFPYVGA
                     GSFTPLSGNGTGAKWGGIINSLNANFTKLRGQHSFRFGVDFRNYREFNNTFTTDVSPV
                     LSFANTYTKATDISTAAPVGAELAAFLLGVPGGYMDRSGSYAQQENYWGLYIHDDIKL
                     TSRLTVNVGVRYEFESPITERFNRSVAHFAYDQASPIEAQARTNYAANPISELPLSDF
                     RVRGGLTFAGTNGNPRALWSGEKNNLMPRIGIAYQLLPKTVLRAGYGIFFDTIGLSSV
                     DSIQTGFSMRTPIQASLDNGLTYIATTANPFPNGLTPPLGAAGGLATNLGQAITFFAE
                     NRKNPYSQRWSMGIQQLLPAQYMIDVSYVGNRGTRLPVTRNLNALPAQYLSTSPVRDN
                     QTIAYLGQSFRNPFAGTDPIYGANITRRGLLLPYPQFGNVNVSEPIGYSWYHSMQVRM
                     EKRFSKSYTLQLSYTWAKTMDATGFLNASDPMPYESISTQDRTHRVVVSGIWELPFGR
                     GRKIGTNLHRALDFFAGGWQLNGMVQRQSGQPLEWGDVWTLFTGDPDAVKLPKDQRSV
                     DRWFNTDAGFNRDVAQQLDANYQIRRSPWRFSSLRADGQARWDFSLFKNFQITEQAKM
                     QFRAECINAWNHPNLFAPVMTPTSSTFGMVTNQDATRTWVMSLKLSF"
     gene            10729..13224
                     /locus_tag="VFQ79_02060"
     CDS             10729..13224
                     /locus_tag="VFQ79_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEU0138459.1"
                     /translation="MRRILIIFGAALICFCARAPAREIDYKSGIEPLFKKRCYACHGP
                     AQQLSGLRLDRRDAALKGGYSGPAIVPGDAQNSRLVELIAGTGALVMPPAGPRLTAGE
                     IELVRSWINQGARWSDEGSTAKPDIVSRRSSSHWSFQPVRKPVLPAAGGSKWPKTPID
                     RFILARLAQLGVEPSPPADRRTLLRRLHLDLTGLPPSFSAMNEFLGDSSPDAVARAVD
                     QLLASPHFGEKWARHWLDLARYADSDGYEQDQVRRDAWRYREWVIQAFNSNMPFDRFT
                     VEQVAGDLLPGAGVEQLIATGFHRNTLTSREGGVDVDQLRAEQVADRTSTTASVWLGL
                     TMDCARCHDHKYDPISQKDYYRFYAFFNNAVEVNHPAPLAGEMGSYLRSRPEFDKRLA
                     AIREKCNVEKLMPGWESEVRAAIQDPEAKLEWTQVADYVRVYVDHGHEIVQKPPPQRT
                     YGEHLALLRVFLKYPGPLASEPEGKGTEFSKGFNEFEKLMLEFPPLSEVPAMARSAAP
                     VKTHIHLRGDFRSPGIEVEAGTPEVLPDLPEGVERDRLALARWLVSGDNPLTARVFVN
                     RVWQELFGAGIVESSDDFGTRGDAPSNPELLDWLAAEFMDSGWNVKQLIRSIVLSTTY
                     GQSSHARADLKERDPSNRLLARQSRLRLPAELIRDSALSVSGLLNPAIGGRSVRPPMP
                     ASVLEVAYRAKWEESQGPERYRRGLYTLLQRSVPYPQLLNFDAPSSLVTCSRRMRSIT
                     PLQSLNLLNDPVFVEASQAFAARILREQNGTDEERMKYAFELALGRRPSTDELSSLVA
                     YVRQKQLSLTEQAAAWFATASVLLNLDEFITRE"
     gene            13238..14680
                     /locus_tag="VFQ79_02065"
     CDS             13238..14680
                     /locus_tag="VFQ79_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0138460.1"
                     /translation="MDEYDFRVIQTRRSFLSQCAGGVGVAALAQLLANDGRAATAEHA
                     ANPLASKLPHFAAKAKNVIFLFMEGGPSQIDLFDPKPEMEHWNGQTLPESMRKNLRFA
                     FIKPTAKVWASGRSFRKHGESGMEFSDWLPHLATCADDICMIRSMHSDQFNHHPGQLL
                     MHCGSPLVGRPSMGAWSLYGLGSESQNLPGFVVLNSGGRGGSGSSGLFGSGFLPSMYQ
                     GVPFASTGDPIPYLSNPDGMTREEQRSRIDLIVQLNQKRFQETGDVEIASRIAAYELA
                     FRMQTAAPELLDLKQETPQTLEMYGADKEPAQAFAKNCILARRLVERGVRFVLLIHTS
                     WDDHSSLTSGHEKNCRSTDQPSAALVQDLKQRGLLDSTLVVWGGEFGRTPLAQTLQPG
                     KESATGRDHHPDAFTMWLAGGGVKRGHVVGHTDELCMNVVENQVHVHDLHATILHCLG
                     MDHKRLTYRHQGRDFRLTDVAGRVIPDILA"
     gene            14683..15669
                     /locus_tag="VFQ79_02070"
     CDS             14683..15669
                     /locus_tag="VFQ79_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0138461.1"
                     /translation="MTSRRTFLTTTALSGFAASLRAAPAARDKALIAVTFDLEMSRNF
                     PTWDQTHWDYEKGNLDEATKRYSLQVARRVRERGAVMHFFAVGRVFEQEDVGWLREIA
                     GMGHSIGNHTYDHVNVLATRAEDIQPRFRRAPWLLDDKPPLDFIRRNVRMTSEAMRQR
                     LGIVPSGFRTPGGFSNGLAGRPDVQRMLKEEGFPWVSGKYARHPLGRLGEAPSPAVYD
                     AIREAQRESQPFRYPETGLLEIPMSPISDIGAFRGGRWPLESFLRAIRVGVEWAVEQR
                     SVYDFLGHPSCLVATDPRMESVDLILDLVRDAGKQAEAVTLDKIAAQFGGDG"
     gene            15666..>16887
                     /locus_tag="VFQ79_02075"
     CDS             15666..>16887
                     /locus_tag="VFQ79_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0138462.1"
                     /translation="MNKAFLLIAACALPGLNAETARTLPNSTRWEFPVDIVAEQYSEL
                     RTFYEREIAKAAAARSHYWAGEAWNAVVRENREHFRRMLGATDPLLEPKPQKTVLAES
                     PSHTVSMLEWPVLPVGNMGATARGSVRAYAVLIVPKSSGPHPAAIVISDAAQSAADLA
                     GLTRALPPAEQTARRLASAGYVVLAPFFVQRRTFCQPWTEDRSWLFRLGYQVGRHLVG
                     TEVQQVTSAVTLLRGMSEVDGDRVAVVGEKQGALTALYAAALDERIRAAVAANYFGTR
                     EKAFEEPEDRTLWKHLIRFGDAEVAGMIAPRALIIDGAVPGTDTEIRRAEGYYARTGE
                     VRPIRATSANAGSISNEAIAALAKVLNPASPAAADYTAALPPERLAQIANTQFQYWQA
                     RLRNLAMEAYAVRQA"
BASE COUNT         3521 a         5017 c         5041 g         3308 t
ORIGIN      
        1 cgcgacgacg gcagtcaggt tgaaaccgac gttgtccagc atcgccagaa tcgcgatgct
       61 gatggccacg actttgacga cgcggcggcc cagcggaatg atggcgatgg cgcccagacg
      121 ctgcgtctgg accatgcgct cgcgaatctg cgcggccgcc aggtcgatca ggcgcaaggc
      181 gaaccacgtc acggcgagga tagaaagcag cacttccacc gtgccgagga attgccgcat
      241 cagtaccgga aagccgagcg gcagccggag aaagtgaaaa acgatgatgg ctaacagcag
      301 tcgaagcggt ccggtggcca accggagcaa ttcgtcgtca gctcccgtag acgtccggaa
      361 taacagcggt cgcagcaggg aacgaaccaa ccaggacagg agccatgcaa ccccataggc
      421 gattggaaga atcgcgagca aagccagcca ttgccatagt ggtaaattga ggagccgcac
      481 ggtcaccaat ggcttgggca acagcctttc aaactccgga aatgtcaggc cctggtaaag
      541 tgccggcgct tgctggacgg tggtattgga gatcttccag actctgattc cgttttccga
      601 aaccctcttt aaaacgaccg gcaccttacg gtccggcatc tcgatcattc ccgcccgttc
      661 ttgattggtg gataactcgg tttctatcga tccgtccggt tcattgctaa cctcgttgag
      721 cctggtggaa agatgcagat ccatcaccgc ctgaagttgc cgcgccgtct cgcggcgttg
      781 cgccgcgccc actccgggta aatactccag atactgggag gcgcggtcgt aatcccgtcc
      841 ctgtgcggct tcgaggaaac cgagaatggc tcccctgggc gtggatcgtc ccagcgggtc
      901 cactggtgcg ggtttaacct cggacggttc ctgctgcgcc agcgcaagga tgctcaacga
      961 gaaaaccaga gcaacaacac gggcagcttt ttgtaaaggt aaatgcattg tatagccgat
     1021 tctgagccgc gcgcgtcagc aagcggtttc tacgtttttc aacaacctct gaaatcaaat
     1081 ctgtttttcc gcaatcgact tggcttgcag gaattgcaac aggtaatcgg gtccgccggc
     1141 cttggagtcg gtgccggaca tgttgaaccc tccgaacgga tgggccccca ccatcgcccc
     1201 ggtacactta cggttgatat acagattgcc gacgaagaaa cggtcgcgcg ccttctggat
     1261 cttttcagga ttccgggtat agacggcgcc tgtcaagcca tactcggagt cgtttgccag
     1321 ctccagcgcg tgatcaaaat ctttggcacg cgttacagcc agcaccggac cgaaaatctc
     1381 ttcctggaaa atagtggctt tcgattcaac gtcggcgatt accgtaggac ggatgaaata
     1441 gccttctccc ggtgggctct cgcccccggc tacgagtcta ccttcctgtt tgcccttctc
     1501 tatgtagctg aggatgcttt ctttggcccg ctggttaatc accggcccca tgtagttggc
     1561 cggttcctcc gcggggccta ctttgagttt ctcgacctta gcggtgagcc gctccaaaaa
     1621 ttcgtcgtac agcttctcac tgacgatggc gcgggaacac gcggagcact tctgtccctg
     1681 gtagccaaat gcggacgcca gcacgccatc cacagcgcta tccaggtccc cttcttcgtc
     1741 gacaatgatg gcatctttgc cacccatctc cgccaccacg cgcttgatcc agacctgccc
     1801 tttttgcggc ttggctgcca gctcgttgat gcgcaggccc acgtcgcgcg aaccggtgaa
     1861 agagacgaaa cgggtcttcg gatgttccac caaaacgtcg cccacggcgg cgccgcttcc
     1921 ggtgagaaag cagaagctgc gatcggggaa tccggcttcc agaagcagcc cggcaaattt
     1981 cgccgcgatc gtcggcgtgt cgctcgaagg cttgatgacg actgtatttc ccgccacaag
     2041 cgctgccgtc gtcatcccta ccagaatggc cagcgggaaa ttccacgggg gaatgatgat
     2101 cccaacacca agcggaagat aaaccatccg gccggtttcg ccgggcagtt gcaccaccgg
     2161 agccggagcc gcgaatcgca gcatctgccg ggcatagtat tcgcagaaat caatcgcttc
     2221 cgcgacatcc gcttctgctt caggccacgt tttccccgcc tcgaagacca gccaggcatc
     2281 aaactccagt ttccgccggc gcaacaggtc cgcggctgcc agcagcattt ccacacggcg
     2341 cccggctgga gtgccgctcc attcggggaa aaacgcatac gccgcgtcca cggccattcc
     2401 cgcgagctcc ggcgtggcct tctggtggat accgatcacc tccgaaggcc tggaagggtt
     2461 cagagaacgg aacttctcct ccgtcttcag gcgcgaaccg gcgattagta actcgtactc
     2521 gcggccgaac tgggcgcgca cggcggcaag ggcggcctcc attgccttgc ggttctccgg
     2581 aaccgggaaa tccgtgtaag cctcgttctt gaagtcgccg ggcacttccg acatggtggt
     2641 ctcccggtct atgtgttctc ctgcggtgca ggttcgcctt ctacaagctt tctgccacgc
     2701 tcgaacaggt ccgccgcttc atcggctatg tggcgcccct tttcgtaaag ctcgcggccc
     2761 cggtcgagaa tttcacgtcc ggattcggca atcgcactcc tgccctcacc cgctttctga
     2821 ctgatccgcc tgcgcgtctc gcctcccgca gcaggtgcat aaagcagcgc gagagcggcg
     2881 ccaacggcag caccggcaat aaaccaaagc actctgcccg atgtattatt catctcttcg
     2941 gccattcgat cctccgaatt tatgatagcc cgtgaaacgc cggaaaagga gcttaatcct
     3001 tcaggagctt ggccccaagc gctccgccca gagtgggaag cagcgtgaag aacacaaaca
     3061 caaatatcag ggagagcagc agaacggtgc ccagccccag cgggttctgc agcacctcga
     3121 tgaactgttc gatatttgcc tctccggcgc cgcgcgcgct cagttgctcc cgatagaaag
     3181 cggcaagccc gccacccctg gcgatcgtca gcacgctgat ggtaaaaaac accgtcgcaa
     3241 tcgcaaagca gaatactccg gtaatccatc ccatgcgaac gcccgcacgc accgtgagcg
     3301 attcgccggt ccggcgccgg taaagccaga ccgcgagcac tccggacgca gtcaaccaca
     3361 gcaacggaaa aaacgccggg gtgggtagtg ttatcaggag cgacgcaatg ccggcggcca
     3421 gaaatcccac acgcaccgcg gcggtattgt ggaaactgat ctccggtttt gccgccaccg
     3481 gccgttcggg ttcgacgatc tcggcgcgga ccggttcctc ttcaggttcg ggtaccggct
     3541 cgctgaataa cgggcggcca cacttgtggc agaaccgtgc gtcggccggt ggaatcgcac
     3601 cgcaggtgca agcttcaggc acacgtccag tgtgccgcac ccgtccgtgc ggggcaagtc
     3661 aagggcggaa ccggcctccg ttctatgcta gagttttgtt ctcatgcggc ccatcactct
     3721 gctcgtactg gcaaatcccg ccgcgcgtta tctggcggca ttggagcgtc ttcccgactc
     3781 gacgcatatt gtcgtcggag acacgaagga agcgttcctc ggcaaggtcg ccggcgcaga
     3841 agtgatcttc accggcatgg gttttgcgcc attgcttcgc gagttgtggc ccgacgcggc
     3901 ccgcgtgcga tgggtgcact cgttttcagc cgggttggat aacgtgctgt ttccggaatt
     3961 gatcgaaagc acggccgtaa tgacgaatgc gcgcggcgtg tttgcgcgtt ccctcggtga
     4021 attcgtcatc gcgtcggtgc tcttcttcgc aaaagaactg aggcgcatga tctggcaaca
     4081 gcgggaaggc gtgtgggagc cgttcgacgt cgaagaggtc cacggacaga cgatggggat
     4141 cgtcggctac ggtgagattg gcagggcagc cgccgaacgc gcgcatgcgt tgggcatgaa
     4201 gaccatcgcg ttgcgccgcc ggcctgagct ttccagcgat gacccgctgg tggaccgtac
     4261 cgtcgggccg gacgggttgt tcgacctgct ccgagaggcc gactacgtgg tggtttcggc
     4321 cgctctcact ccctccaccc gcgggatgat cggcgaggcg gaattgaatg caatgaagag
     4381 atccgccgtg ctgatcaatg ttggccgtgg gccggtcgtg gtggagtccg cgcttgttcg
     4441 cgctcttcgc gaaaaccgga ttaagggtgc ggcgctcgac gtattcgacg aggagccgct
     4501 cccggtggga cacccctttt acgacttgga aaacgtgttg ttatctcctc actcggcgga
     4561 ccatacggcc acgtggctcg atgaggcgat gcagcttttt gtcgagaatt ttgagcgctt
     4621 cgtcaaagga gagccgcttc acaacgtggt tgacaaatca gaaggatact gagatgaaaa
     4681 atcgggtctt gttgtttctt cttatagcgg caacagcatt cgcgcagagc tacaaaccgg
     4741 ctccggagaa cctcgaggcg cgcaaatggt ttcaggacgc aaaattcggg atgttcatcc
     4801 attggggcgt ttacagcgtg ctcggagatg gggaatgggt catgaacaac cagaagatcc
     4861 cgatcccgga gtacgagaag ctgccgccaa agttcaatcc caccaaattt gacgcggccg
     4921 agtgggtttc gcttgcgaag aatgccggaa tgaagtacat caccatcacc agcaagcatc
     4981 atgacggctt cgcgatgtgg gccacaaaac agagtaagtg gaatattgtc gacgccaccc
     5041 cttacgggaa agatccgctc aaaatgctgg ccgatgaatg caggcggcag ggaatcaagc
     5101 tcttcttcta tcattcacaa ctcgactggc atcacccgga ctactatccg ctggggcgca
     5161 caggccacgc atccggacgg cccgccggcg gcgatttcaa ccgctacctg gactacatgg
     5221 atcgccagct tcgcgaactg ctgaccggct acggtgagat cggcggcatc tggttcgacg
     5281 gctggtggga caaacccgat gcggactggc gcctggacaa aacctactcg ctgatccaca
     5341 gcctgcaacc ggccgcgttg gtgggcagca atcaccatcg caagccgttc gatggcgagg
     5401 atttccagat gttcgagaaa gacctgccgg ggcagaatac taccgggttc agcggggagt
     5461 cggaaatcgg aaacctgccg ctcgaaacgt gcgacaccat caacggcgca tggggctaca
     5521 acaagaacga caagcgattc aaaagttcgc agcagcttat ccaatatttt gtgaaagcag
     5581 cggggcataa tgccaatttc cttctgaatg tggggcccac accggagggt acgattcagc
     5641 cggaatttgt cgaaaggctc caggcgatcg gcgagtggat gcgcaagaac ggcgagacga
     5701 tctatggaac gcggggaggc ccggttgccc ctcgaccctg gggcgcgatg acgcagaagg
     5761 acaacaggat ctttgttcac gtgctggatt ggaatgatga gctactggcg cttccgccaa
     5821 tgccgaagat caagagcgcc acgttgttcg ccaccggcgc cacggttaag atccaggccg
     5881 tggatggagg tttactcctg cggcttccca aaaccggcag agaccccgtc gatacgatcg
     5941 tggtgctgga gaaacagtga acaacctgcc tgttacagtc gacgagattc gcgccgcggc
     6001 ggaacgcatc cgcggcgtag cctaccggac gccggtgatg acctctcgaa gcttcgatag
     6061 cgtggccggg attcatgctt tttttaaatg tgagaacctg caacgcggcg gcgcattcaa
     6121 gatccgcggg gcggcgaact tcatcttctc tatcccaaaa gccgacctca gccgtggggt
     6181 ggtggctttc agttccggaa accatgccca ggcggtggcg attgcggccg gcgtggtgaa
     6241 cacgcctgca accgttgtca tgccgtccta cgcaccgcag tctaagcttg ccgccacccg
     6301 cggttatggc gccaaggtca ttgagtacga ccagagcagc gagccccggg aagcgatcgc
     6361 cgcacgcatc gcagagaaat ccggggcgac cgttgtgcct ccgttcgatc acccgtggac
     6421 aatcgccgga caaggcacag ccgcgttgga actgctggcg gactatccgg atctcgatgc
     6481 gctgctggtg ccggtcggcg gtggcggcct catctccgga tgcgccatcg cggcaaagag
     6541 cacccggccg gacatccgca tcttcggaat cgaaccagcc gcggccaacg atgcatttct
     6601 ctccaagcgt gccggccgcc gcgtgcgcat cgagcggacc gataccatcg cggacggttt
     6661 gcgctctccc gaaccgggag agctgacgtt tccggtgatc gagcaactgg tggaagatat
     6721 cgtgctcgtg agtgaggacg aaattaaatc cgccgtgaaa ttcctgctgg cccgcttgaa
     6781 aattctcgtt gaaccgagcg gagccgtggg ggccgccgct ctactctccg gcaagctacc
     6841 gccggatgta cgcaccgctg gagttattct ttcaggaggc aatattgacc tggagcaact
     6901 cgcacggttt taggggcgtc tttgattgac ttacccaact gtctggtata taaaagatcc
     6961 agtcgctttg tgggagttag ggtctgattg tctttttatg cctcgtcgag gcactcacca
     7021 gaacgcggcg tcagaagcac ccctgctgcg attcaacgca cccaggtacg gagattgcac
     7081 ttatgcagat tggcgtatca cgtctattcg ctacattctt gatagcagtt tctctcttga
     7141 tgcccgtatc gctccgcgcg caggacgcgc gtggttcgat cgtcggccgt gtcgccgact
     7201 ctaccgacgc cgtaatcgca ggcgccgaag tgcgggcgac caacgtggca accggggttg
     7261 ccgcggtctc gaaaaccaac gaagccggca attacgttat tgcatacctg ctgccgggga
     7321 gatacacggt agaggtggaa ttcgcgggct tcaagaaatt cgtccggcaa ggaatcgaag
     7381 ttcgcgtcgg tgacaccgta aacattgaag ccgttttgga aatcggaaac caggcggaga
     7441 cggtggtggt aaccgccgag acgccattgc tggagacggc cgacgcttcc ctgggccagg
     7501 tggtggacga acgccggatc gccgaattac catctttcgg cggcgcgccc tacaacctga
     7561 ccctgatggc gcccggcacc atcaactcga caaatcttcg ccagcggtat gtcggtacac
     7621 ccggtgccca gggagatttc gcggtagacg gcgccggcat gagaaacaac gaattcacca
     7681 ttgacggcgt cgccaattca ctcgacaagg gtatcgtgtt tgtgccgccg caaatgtcgg
     7741 tcagcgaatt caaagtacag agcgtctcct acgatgccag catcggccac accgccggag
     7801 cgctggtgaa tgtgagcctc aagagcggta ccaacgaatt gcatggagaa gcacattggt
     7861 ttgtgcgcaa ccggatcttc gatacgccga cgctgttcca gaaccgggtg ggccaggaag
     7921 ttccacctta tcaggaccat cggtacggag taagcggcgg cggacccgtg aagattccga
     7981 agctctacaa cggcacgaac aggaccttct ggttttatct gtgggaggcg aacaaggttc
     8041 agtatgcgta cgatttccag aacaccgtgc ccaccgctga aatgaaacgc ggcgatctgt
     8101 cgcctctgct ggcactggga gccaactacc aggtttatga ccctgccacc acggtgttct
     8161 ccgggggccg gttcacccgc cagccatttc ccaacaacat cattcccgcg aaccgcctgg
     8221 acccgacagc gcagaagatt atggagtact ggccggctcc gaatcagacg ggcacgcgtg
     8281 aattccggaa caatcatttt gtcagctatg cgggcccctt tcccgtgtgg acgcacctgg
     8341 ggcgcttcga ccattctttc aatcccaacc accgcctcta cgcgcgagtg atgcgggagg
     8401 gtttctggag cgcgtcgaac aaaaggttcc tgtcggatta cgacggcatg caatataacc
     8461 aggacaagtg gggattcgca ctggacgacg tatatgtctt caaccccgaa ttcttcatga
     8521 acctccgcta cggctttacg taccgctggg gggcctccta caaattcagc caggggttcg
     8581 acctggcgac actcgggttc tcgcccaatc tgatctcgca actgccggac aagtcgctgg
     8641 cgacttttcc ttatgtcgga gcgggctcgt tcaccccgtt gagcgggaat ggaacaggcg
     8701 caaagtgggg aggaatcatc aactcactga acgccaactt caccaagctc agagggcagc
     8761 acagcttccg tttcggcgtg gatttccgca actaccggga gttcaacaat acatttacca
     8821 ccgatgtttc gccggtgctg tcgttcgcaa acacttatac aaaagccacg gacatctcta
     8881 ccgcggcgcc ggtgggcgcc gaactggctg cgtttctgct cggagttccg ggcggctaca
     8941 tggaccgttc cggcagttat gcacaacagg agaactactg gggtctctat atacacgacg
     9001 acatcaagct gacttccagg ctaaccgtga acgtcggcgt gcgctacgaa ttcgagtctc
     9061 cgataaccga acgcttcaac cgttcggttg cccacttcgc gtacgatcag gcaagcccca
     9121 tcgaggcgca ggcgcgcact aactatgccg ccaatccgat ttccgaactt ccgctcagcg
     9181 acttccgcgt acgcggcggc ctgaccttcg ccggtacgaa cgggaatcct cgcgcgctct
     9241 ggtcgggcga aaagaacaac ctgatgccgc gcatcggcat tgcctatcaa ctgctgccga
     9301 aaacggtgct gcgggccggc tacggaattt tcttcgatac gatcgggctc agctccgtgg
     9361 attcgattca aaccggattc agcatgcgca cgccgatcca ggcctcgctc gataacggtc
     9421 tcacttatat cgccaccacg gccaatcctt tcccgaacgg attgacgccg ccattgggcg
     9481 cggcgggcgg cctggccaca aacctcggac aggcgatcac ctttttcgcc gagaaccgta
     9541 agaacccgta ttcacagcgg tggtctatgg gcatacagca actgctgccg gcccagtaca
     9601 tgatcgacgt ctcttacgtc ggcaatcgcg gcacgcgtct gcccgtgacc cggaatctga
     9661 acgctttgcc ggcccaatac ctgagcacgt cgccggtacg cgacaatcag acgatcgcct
     9721 atctcggcca gtctttcagg aacccctttg cgggaactga tcctatctac ggcgcaaata
     9781 tcacccgccg gggtctgctg ctgccatatc cgcaattcgg caatgtaaat gtcagcgaac
     9841 cgatcggcta ttcctggtat cactcgatgc aggtacgcat ggagaagcgc ttctcgaaga
     9901 gctacacttt gcagctctcc tacacctggg cgaaaaccat ggacgccacc ggttttctaa
     9961 acgccagcga tccgatgccc tatgagtcga tttctacgca ggaccgcaca catcgggttg
    10021 ttgtaagtgg gatttgggag cttcccttcg gccgcggccg aaaaattggc accaaccttc
    10081 accgcgcttt agacttcttc gccggcggct ggcaactcaa cgggatggtt caacgccaga
    10141 gcggacaacc gctcgaatgg ggcgatgtct ggaccctgtt cacgggagat ccggatgccg
    10201 tcaagttgcc gaaagaccag cgcagcgtgg atcgctggtt caacaccgat gccggtttca
    10261 atcgagatgt tgcgcagcaa ctggacgcca attaccagat ccggcgttcg ccctggcgct
    10321 tcagcagcct gcgtgccgac ggccaggcgc ggtgggactt ctcgctcttc aagaatttcc
    10381 agatcaccga acaagcgaag atgcagttcc gtgcagaatg catcaacgcc tggaaccacc
    10441 cgaatctgtt tgcgccggtg atgacgccga cgagcagcac tttcggcatg gttacgaatc
    10501 aggatgccac ccgcacgtgg gtgatgtcgc tgaagctgtc cttttagtcc gttcgggcag
    10561 ttgaaaacct ccagcgggca ccccacagac gtggagcgcc cgccctctaa atctccctcc
    10621 tccgcttgtg catagcaagt ttagatggtg ctgatgcgat gattctatgt ggcgcttcgg
    10681 gctgcctgat ttatcatgaa gggcagccaa acctgaaagg cgctctggat gaggcgcatc
    10741 ctgatcattt tcggcgcagc gttgatctgt ttttgcgcgc gcgcaccggc gcgcgagatc
    10801 gactataagt ccgggatcga acctctgttc aaaaaacgtt gctatgcctg tcacggaccg
    10861 gcacagcagt tgagcggcct gcgcctggat cgccgcgatg ccgcgttgaa aggcgggtat
    10921 tcaggaccgg caatcgtacc cggcgatgcg cagaacagca ggctggtcga gttgatcgcc
    10981 ggaacgggcg ctctggttat gccgccggcc gggccgcggc tcaccgcggg cgagattgaa
    11041 ctggtccggt cgtggatcaa tcagggtgcg cggtggtccg atgaagggag cacggcgaag
    11101 ccggacatcg tttcacgccg gtcatcgtcg cattggtctt tccaacccgt gcgaaagccg
    11161 gtgcttcccg cggcaggcgg cagcaagtgg ccgaagacgc ccatcgaccg cttcatcctg
    11221 gcaagactcg ctcaacttgg cgtcgaaccc tcgcctccgg cggaccggcg gaccctgctc
    11281 cggcggctgc atctcgattt gaccggactg ccgccgtctt tctccgcaat gaacgagttc
    11341 ctgggtgact ccagtcccga tgcggttgca cgcgccgtcg accaacttct agcttcaccg
    11401 cacttcggtg aaaaatgggc gcgccactgg ctcgatctcg cacggtatgc cgacagcgat
    11461 ggttatgaac aggaccaggt tcggcgggac gcatggcgtt atcgcgaatg ggtgatccag
    11521 gcattcaaca gcaatatgcc attcgaccgg ttcacggttg aacaagtcgc cggagacctg
    11581 ctccccggcg cgggcgtcga gcagcttatc gcaacgggct tccatcgcaa tacgctgacg
    11641 agccgggaag gcggcgtgga cgtggaccag cttcgggccg agcaggtagc cgaccggaca
    11701 agcacaaccg cctccgtctg gctgggactt accatggatt gcgcgcgctg tcacgatcat
    11761 aagtacgatc caatttcgca aaaggattat taccgcttct acgccttctt caacaatgcc
    11821 gtggaggtaa atcatcccgc gccgctcgcg ggcgaaatgg gctcttatct ccggtcccgc
    11881 cctgaattcg ataagaggct cgcggccatc cgggagaagt gcaacgtcga gaaactgatg
    11941 cccggttggg agtccgaagt gcgcgcggcc atccaggatc cggaagcgaa gctcgaatgg
    12001 acacaggttg ccgactatgt acgggtctat gtcgatcatg ggcacgaaat cgtgcagaag
    12061 ccgccgccgc aaaggacgta tggcgagcat ctggctctgc ttcgcgtgtt cctcaagtat
    12121 ccgggacccc tggcgagcga gccggaggga aaaggaactg agttcagcaa gggcttcaac
    12181 gagttcgaaa agctgatgct ggaatttccg cctctcagcg aagtaccggc tatggcgcgg
    12241 agtgcggcgc cggtaaaaac acacattcac ctgcgcggcg atttccgaag ccccggtatt
    12301 gaagttgaag ccggaacacc cgaggtgttg cccgatcttc cggaaggagt ggagcgcgac
    12361 cgccttgccc tggcgcgctg gctcgtatcg ggagacaacc cgctcactgc ccgcgtattc
    12421 gtgaatcgcg tgtggcagga actgttcggc gcgggcatcg tcgaaagctc ggatgatttc
    12481 ggcacacgag gggatgcgcc gtcaaaccct gagctattgg attggctggc ggccgaattc
    12541 atggacagcg gatggaacgt aaaacaactc ataaggagca tcgttctctc gaccacgtac
    12601 ggacagtcct cgcatgcacg cgccgatctg aaggaacgcg atccttcgaa ccgcctgttg
    12661 gcacgccaat ccaggctgcg gcttccggcc gaactgattc gcgacagcgc cttgtcagtt
    12721 tccggtttac tgaatcctgc catcggtgga agaagcgtcc ggccgccgat gcccgccagc
    12781 gtactcgaag tggcatatcg cgcgaaatgg gaggaaagcc aagggccgga gcgttaccgc
    12841 cgcgggctct atacactgct tcagagatct gtgccctatc cgcagctcct gaattttgat
    12901 gcgccaagtt cgcttgtgac gtgcagccgg cgaatgcgtt cgattactcc actgcaatca
    12961 ttgaacctgt tgaatgaccc cgtcttcgta gaagcgtcgc aggcgtttgc cgcgcgcatt
    13021 cttcgggaac agaacggaac ggacgaagag cgcatgaagt acgctttcga gctggcattg
    13081 gggagacgtc cgagcacaga cgaactgtct tcgctcgtag cttatgtccg gcagaaacag
    13141 ctttcgctta cggagcaggc tgcggcctgg tttgcgacgg cgagcgtact gttgaacctg
    13201 gacgaattta ttacgcgcga gtaagcggaa aacgaaaatg gacgaatacg atttcagagt
    13261 gattcagaca cgccgcagct tcctctcgca atgcgccggc ggagtcggtg tagcagcgct
    13321 ggcgcagttg ctggcaaacg acggacgtgc tgccacagcg gaacacgcgg cgaatcctct
    13381 tgcatccaag cttccgcatt tcgcggcaaa agccaagaac gtcatctttc tgtttatgga
    13441 gggcgggccc agtcagatcg acctgttcga tcccaagccg gaaatggagc actggaacgg
    13501 ccagacgctg cccgaatcca tgcgaaagaa cctgcggttc gcatttatca agccgacggc
    13561 gaaagtatgg gccagcggaa gatcgttccg gaagcacggc gaatcgggca tggagttctc
    13621 cgactggttg ccgcacctgg ccacctgcgc ggacgacatc tgtatgatcc ggtccatgca
    13681 cagcgaccag ttcaatcatc atccgggcca gttgctgatg cactgcggga gtcccctggt
    13741 tgggcgcccc tccatgggag cgtggagcct gtacggcttg ggcagcgaat cgcagaacct
    13801 gccgggattc gtcgtattga actccggagg gcgagggggc agcggcagtt ccggcctgtt
    13861 cggcagcggc tttctacctt cgatgtatca gggagttccg ttcgcaagca ccggcgatcc
    13921 gattccgtat ctttcgaatc cggacggcat gacgcgcgaa gagcagagat cgcgcatcga
    13981 cctgatcgtc caactcaatc aaaagaggtt tcaggaaaca ggcgacgtgg agatcgcctc
    14041 gcgcatcgcc gcctatgagc tggcattccg gatgcaaacc gccgccccgg agcttctcga
    14101 cctgaagcag gaaacaccac agacgctcga gatgtacgga gcggacaagg aaccggcgca
    14161 ggcgtttgca aagaactgca ttctggcgcg gcggctcgtc gagaggggag tgcggttcgt
    14221 tctgctgatt catacgagct gggacgatca cagcagcctg acgtcgggtc atgagaagaa
    14281 ctgccggagt acggaccagc catcggcggc tctcgtccag gacttgaagc agcgcgggct
    14341 gctcgacagt acgcttgtag tctggggcgg cgagttcggc cggacaccgc tggcacaaac
    14401 tcttcagccg ggcaaggaga gcgccaccgg gcgcgatcat cacccggatg cgtttacgat
    14461 gtggcttgca ggcggaggcg taaaacgcgg acacgtagta ggccataccg acgaactatg
    14521 catgaatgtg gtagagaacc aggttcatgt tcacgatctc cacgcgacca tcctgcactg
    14581 cctgggcatg gaccacaaac gtctgaccta ccgtcaccag ggccgggact ttcgtttgac
    14641 ggacgtggca ggacgcgtga ttccggacat actcgcttga tcatgacctc ccggcggaca
    14701 ttccttacga ccacagcctt gagtggattc gcggccagtc tgcgggccgc accggcggct
    14761 cgcgataaag cgttgatcgc cgtcacgttc gatcttgaga tgagccggaa ctttcccacc
    14821 tgggaccaga cgcattggga ttacgaaaag ggcaatcttg atgaagcgac aaagcgctat
    14881 tcgctacagg ttgcgcggcg ggtgcgcgaa cgcggagcgg tgatgcactt ttttgctgtc
    14941 ggcagggtct tcgaacagga agatgttggt tggttgcggg aaatcgccgg catggggcat
    15001 tccatcggca accatactta cgatcacgtg aatgtgctag ctacccgggc cgaagacatc
    15061 caaccgaggt ttcgccgcgc tccgtggctg ctcgacgata aacctccgct cgatttcatt
    15121 cggcggaatg ttcgtatgac atcggaggcc atgaggcagc ggcttggaat cgtccccagt
    15181 ggattccgca cgcctggcgg gttttccaat ggacttgccg gacggcccga cgtgcaacgg
    15241 atgctgaagg aagaaggttt cccgtgggtt agcggcaagt acgcgagaca tcccctgggg
    15301 cgtttgggag aagctccgtc tccggcagtc tatgatgcga tccgcgaagc gcaacgcgaa
    15361 tcgcagccat ttcgctaccc ggaaacaggg ttgctcgaaa ttccgatgag ccccattagc
    15421 gacatcggag cgtttcgcgg cggccgatgg ccgctcgaaa gctttctgcg cgcgatccgc
    15481 gtgggtgtgg agtgggccgt cgaacagcgg tcggtctacg attttctggg gcatccatcg
    15541 tgcctggtgg caacggaccc tcgcatggag agcgtcgacc tgatcctgga cctcgtgcgc
    15601 gatgccggca aacaggccga agcggtaaca ctcgataaga tcgccgccca attcgggggt
    15661 gacggatgaa taaagcattc ctgctcatcg ctgcgtgtgc cttgccgggg ttgaatgcgg
    15721 aaacggcccg aactctcccg aactcgaccc gctgggaatt cccggtggat atcgtcgcgg
    15781 aacaatacag cgaattgcgt accttctatg agcgcgaaat cgcgaaggcc gcggcggcgc
    15841 gcagccacta ctgggctggc gaagcttgga acgcggttgt tcgcgagaac cgcgagcatt
    15901 tccgccggat gcttggcgca accgatccgc tgctcgaacc caagccgcag aagaccgttc
    15961 tcgccgagtc gccatcacac acggtgtcga tgctggagtg gccggttctt ccggttggga
    16021 acatgggcgc tacagcacgg ggcagcgtgc gcgcctacgc ggtgttgatc gttccgaaat
    16081 cctccggccc gcatcccgcg gcgattgtaa taagtgatgc ggcacaatcg gctgcggacc
    16141 ttgcaggttt gacgcgagcg ttgccgccgg cggaacagac ggcacgaaga ctggcttcag
    16201 ccggctacgt cgtgctcgca ccgttcttcg ttcagcggcg cacgttttgc cagccatgga
    16261 ccgaggaccg tagctggctg ttccggctgg gctaccaggt aggccggcac ctcgtcggca
    16321 cggaagtgca gcaggtgaca tcagcggtta cgctgttgcg cggcatgtct gaggtggatg
    16381 gggaccgcgt cgccgtggta ggagagaagc agggcgcact cacggcgcta tatgccgcgg
    16441 cactcgacga acggatccgt gcggccgtgg ccgccaacta cttcggcacg cgggaaaagg
    16501 ctttcgagga gccggaagac cggaccctat ggaagcacct tattcgtttt ggcgacgcgg
    16561 aagtggccgg gatgatcgca ccgcgtgcat tgatcatcga cggcgccgtc cccggaacag
    16621 ataccgagat acgccgggcg gaaggttact acgccaggac tggcgaggtg cgcccgatcc
    16681 gtgcaacatc cgctaatgcg ggctccatca gcaatgaggc aatcgcggct ctggcaaaag
    16741 tgttaaaccc ggccagtcct gccgcagcgg attacaccgc ggcgttgccg ccggagcgcc
    16801 ttgcgcagat tgcgaatacc cagtttcaat actggcaggc acgtctccgc aatctggcaa
    16861 tggaggccta tgccgtgagg caggcgg
//
LOCUS       DASTMK010000048         6502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_34666, whole genome shotgun sequence.
ACCESSION   DASTMK010000048 DASTMK010000000
VERSION     DASTMK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34666"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1545
                     /locus_tag="VFQ79_02080"
     CDS             154..1545
                     /locus_tag="VFQ79_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU0138463.1"
                     /translation="MPAADARESAWCSGGFILTPPAGILDNDNARLSVTYRNQAAYNA
                     ALLTLHSYRAVFGMKRLLIVFLAIGLLQSLVFGASTRKKTSSKRTVKKTSVQKKTVQK
                     KKVSSNVKRVKKAAPKHTKRRVYRRSVSSVWKTPTFADSTSHDQINGEDLEIRRAAVE
                     ALGPYNGSVVVVDPSNGRILSMVNQSLALSTGFIPCSTVKLITGLAGLNEGIIDRDTQ
                     MRLSKRSSLNLTSALAHSNNTYFANIGLKLGFEKVRHYAQMLGLGEKAGLNIEGESPG
                     ELTTQPPKRGGVGMMTSFGEGISMTPLQLAAILSSIANGGKLYYLQYPTNPEELAAFE
                     PRLKRDLALDPWMPEIKYGMRAATMYGTARRANYGPDEPVWGKTGTCTDYGKAAHMGW
                     FGSFNEVGGKQLVVVVMLTGGKPVNGPLASGIAGTFYKLVATRGYFPGQPSTVPIALI
                     STEKCCTLELSAN"
     gene            complement(1576..3435)
                     /locus_tag="VFQ79_02085"
     CDS             complement(1576..3435)
                     /locus_tag="VFQ79_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138464.1"
                     /translation="MLSVALAQPALEPVKTSITVVENISADAPASVSVLNSEVLSETP
                     GVNLDDRLRSVPGFSLFRRSSSLVAHPTTQGISLRGIGSSGASRTLVLWDGSPVNDPF
                     GGWVYWTRFAPEEIDRVEVSRGASTSVFGDRALGGAIAMFSRQPEPRRITAAYEAGNH
                     STHSVSAGVSNVWNEFALSASSRGFMTGGYFTVPDEWRGSVDRRATVRFLSGDTHLDY
                     FNSHDRFFLNLNLLAEERGNGTGLQRNSTGLGTLAGHYHREQQNDSFSLLAYHTRGEF
                     RSAFSSITPDRNLENLTFRQSVPSEAIGAAALWRHKDSWWNSLFGTDAQRVEGYSIDS
                     PAGGGKRIGGGTQFQQGVFAQLDATARRAKFFLGGRQQFASGGHSFFSPSGGVAVNLG
                     HFRARSSAYKAFRAPTLNELFREFRVGNAVTRANADLRPETLTGVETGLDWSGEFAQV
                     SFSLYRNSLQDLIANVTLENTSNQIIRQRRNAEEALSRGVEMNVRGRWRRWNGELGYL
                     FADSRFSDGRRVPQIPRHQGSAQLSWHHGGTLASAGVRSYSNQLEDLPDRPLPGFTTV
                     QLYLRQQVAPRLAAFAAFENLLDREYIVGLTPTPVTGQPLLWRAGLRWEGQIP"
     gene            complement(3371..4492)
                     /locus_tag="VFQ79_02090"
     CDS             complement(3371..4492)
                     /locus_tag="VFQ79_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0138465.1"
                     /translation="MSVRPIWKALRDALEMALRHQVTAGGLLFTFTISLVGVAAFVSA
                     NNLLFLLFAALLSTLLVSGFVSRLSLSGLELDFVLPEHISARARTSARILIRNSKSWM
                     PSFSIHLAGSPDTGMSSVVYFPSIPRNATVEEGVEVFFTRRGIYRENSFQFSTRFPFG
                     FTERRIRVRLRREILVYPSIGPQSGFEALLQALSGEIQAYYRGRGHDFYRIRPYEAFE
                     SARHVDWKATAHTGDLQVREFAQEQEHLIEMFLDLEVAPERLEWFEQAVDCCAFLVWH
                     VSERGARIRFRTQDFDLTLPEEGDVYTILKYLAETSPLKGKAPPAPGDERSFKIVFTT
                     IPDKFLEAGWTSARMLDTSAFGGAGATGTGAGENLHHRS"
     gene            complement(4500..5993)
                     /gene="dacB"
                     /locus_tag="VFQ79_02095"
     CDS             complement(4500..5993)
                     /gene="dacB"
                     /locus_tag="VFQ79_02095"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU0138466.1"
                     /translation="MGRVALLLVAQTLVAAGPPLSARIEVLLKNSPVAERSYWGIQVL
                     DLRTGRLLYNRNQDKLFTPASNTKLFSTALALQKLGPDHRFYTRVVSTVQPASDGILR
                     SDLLLIGGGDMTLSARSLPYRPGPVEGDPLAPLASLADQVVAAGVNRIEGDVVGDDTA
                     YVWEPYPDGWTVDDAIWEYGAPASALIVGDNSIRISLQPSASPGGLAGLLVSPAFEYY
                     VFQNRVETTAGGTRKISVHRLPASRLVRLWGTLPSSSRPSVQYLAVDDPALYAAEVFR
                     DLLIRRGVRIKGEAVARHLLPGEIGDLTRGPNPRFLASGIELAARTSPPLIESLRVID
                     KISQNLQAEVVLREVGRMRRDLGSRDAGLAELEAFLAEIGILPDAYHFEDASGLSRKT
                     LVSPSAVVSLLRHMYHSPQRDAWLRLLPVGGEDGTLEHRFRGTAAAGLVRAKTGTISH
                     VSALSGYVDGRRGGQLAFSILANHYDVPAASVRDLTDRIVNLLASQR"
     gene            complement(6002..>6502)
                     /locus_tag="VFQ79_02100"
     CDS             complement(6002..>6502)
                     /locus_tag="VFQ79_02100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0138467.1"
                     /translation="SPETSLLLLSLYWLTLLVGRLTVHFVIPRMKRGWLLAGSAACAV
                     FGCLILSFTNNRFGAITAIVLLGLGFAVVYPLLVEKIGLRFPNYHPGLFNGISAVASV
                     GGLLAPGTLGYFAEFWGIRVVMLLPLLGTFAVFVLVLMIWLTAKLSGGAEIRIKETAD
                     KPTGLG"
BASE COUNT         1443 a         1872 c         1944 g         1243 t
ORIGIN      
        1 ggtgcggaag aagtatgcgc ccggcgcaca gcctgtgggg agttgacgct acgtagaaaa
       61 ccgcttgcgc gcgcgcgcgg ctctgtttct gagccgtgac cgtgagggag cggtggctgc
      121 aacgcgtttt tcaacaagct cctaaagaat aaagtgccgg cggccgatgc acgggagagc
      181 gcatggtgct ctggaggttt tatattgacg ccgccagcag gtatcctgga taatgataat
      241 gctcggctat cagtcacata ccgcaatcaa gcagcgtata acgcggcgtt gctcacattg
      301 cattcatacc gcgcggtctt cggaatgaaa cgtcttctta ttgtttttct ggctatcggc
      361 cttcttcaaa gtttagtatt cggcgcgtcg acgcgaaaaa agacctccag caaacggacg
      421 gtcaagaaaa cttccgttca gaaaaagaca gttcaaaaaa agaaagtctc ctccaacgtt
      481 aaacgtgtta agaaagcggc gcctaaacac acgaagcggc gtgtttaccg aaggagtgtc
      541 tccagtgtct ggaaaactcc cacctttgcc gattccacct cgcacgatca gatcaacggc
      601 gaggatcttg aaatccggcg ggcggctgtc gaggcgcttg ggccttacaa cggttccgta
      661 gtggtggtgg accccagcaa cgggcgtatt ctcagcatgg tgaaccaaag tctggcgctg
      721 tcgacgggct tcatcccgtg ttccaccgta aaactcataa cgggcctggc gggattgaac
      781 gagggcatca tcgatcgtga tacgcagatg cggctcagca agcgatccag cctgaacctg
      841 acatcggcgc tcgcgcactc caacaacacc tactttgcca acatcggctt gaagttgggc
      901 ttcgagaagg ttcgtcatta cgcccaaatg cttggcttgg gtgaaaaggc cgggctcaac
      961 atcgaaggtg aaagtcccgg ggagctgacc acgcagcctc caaaacgggg cggtgtcgga
     1021 atgatgacca gcttcggcga gggcatctcc atgacgccgc tgcaattggc tgccattctg
     1081 tcctctattg ccaacggcgg caagttgtac tatctccaat accctacgaa cccggaagaa
     1141 ctcgcggctt tcgaaccacg attaaagcgg gatcttgctt tagatccgtg gatgcccgaa
     1201 atcaagtacg gtatgagagc ggcgaccatg tacggtaccg cgcgccgcgc aaattacggc
     1261 cccgacgagc ccgtctgggg caagacgggg acctgcaccg actacggcaa ggctgcccat
     1321 atgggctggt tcggttcttt caacgaagtt ggcggcaagc aactggtagt ggtcgtgatg
     1381 ctcaccggcg gcaagccggt caacggcccg ctcgcttccg gtattgccgg aacattctat
     1441 aagcttgtgg ctactcgcgg ttacttccct ggacaaccct ccacggtgcc gattgcgctg
     1501 atttcgacag agaagtgctg cacgctggag ctttcggcga actgacgacc tggccctggc
     1561 ggtttctgtt accggtcagg ggatctgccc ttcccaacgt agtcccgcac gccacagcag
     1621 cggctgtcct gtaaccggcg tcggagtaag tccgacgatg tactcgcggt ccaggaggtt
     1681 ttcgaatgcg gcgaacgccg caagccgggg tgccacctgc tgccggaggt acagttgcac
     1741 cgttgtgaaa cccggcagag ggcgatccgg caggtcttcc agttgattgc tgtagctgcg
     1801 tactccggcg gaagcgagag tcccgccgtg gtgccacgac agttgggccg aaccttgatg
     1861 ccgcgggatc tgcggcacgc ggcgcccatc gctaaatcgc gaatcggcaa acaggtatcc
     1921 caattcgcca ttccatcgcc gccagcgtcc gcgcacattc atttcgacgc cccgggacag
     1981 ggcctcctcc gcattccttc gttgccggat gatctgattc gaggtgtttt cgagagtaac
     2041 attcgcgatc agatcctgta aggagttgcg atacaggctg aagctgacct gtgcaaattc
     2101 accggaccaa tcgaggcccg tctcgacgcc tgtcagcgtt tcgggccgca gatccgcatt
     2161 cgcacgcgtc acggcattgc ccacccggaa ctcgcgaaac aactcgttca atgtgggtgc
     2221 gcggaaagcc ttgtaggcgg agctgcgggc gcgaaaatgg cccagattga cggcgactcc
     2281 cccgctggga ctgaagaagg aatgaccgcc actggcgaac tgctgccggc ctcccagaaa
     2341 gaatttggca cgccgtgcgg ttgcatccag ttgcgcaaat accccctgct gaaactgcgt
     2401 tccaccgccg attcgttttc cgccgccggc gggggaatcg atgctatagc cttcgacacg
     2461 ctgtgcatcg gttccgaaca gcgaattcca ccaggaatct ttatgacgcc agagagccgc
     2521 cgctccgatg gcttccgagg gcacactttg ccggaacgtc agattttcca ggttgcgatc
     2581 cggggtaata gacgaaaagg cgctccggaa ctctccccgt gtgtggtagg caagcagtga
     2641 gaaggagtcg ttctgctgct cgcggtggta gtgtccggcc agcgttccga ggcctgtgga
     2701 attcctttga agcccggttc cgttaccgcg ctcttccgca agcagattca agttcaggaa
     2761 gaaccgatcg tgtgagttga agtagtcaag gtgggtgtcg ccagatagaa atcttacagt
     2821 ggcccttcga tccacactgc cccgccactc atccggaacc gtgaagtacc cgccggtcat
     2881 aaaaccccgg ctgctggcag aaagcgcaaa ctcattccag acattagaga ctccggcaga
     2941 cacgctatgt gtgctgtggt tgcccgcttc gtatgcggcc gttatccgcc ggggttccgg
     3001 ctgccgcgaa aacatggcga tagccccacc gagcgcgcgg tctccgaaga cactggtgga
     3061 agctccgcgc gagacttcga cccggtctat ctcctcgggt gcgaaacgcg tccagtagac
     3121 ccatccaccg aacgggtcgt tcacggggct tccatcccat agcacaagcg tccggctggc
     3181 gccgctcgaa ccaatgccgc gcaacgaaat gccctgcgtg gtgggatgcg caacaaggct
     3241 ggacgagcgg cggaagagac tgaatcccgg cacgctgcgc agccggtcgt cgaggttcac
     3301 gccgggtgtc tcgctcaata cctccgagtt caggacggac accgatgccg gggcgtcggc
     3361 gctgatgttt tcaactacgg tgatggaggt tttcaccggc tccagtgccg gttgcgccag
     3421 cgccaccgaa agcactggta tccagcatgc gagcgcttgt ccaaccggct tcgagaaact
     3481 tatccggaat cgtcgtgaat acaattttaa aacttcgttc atcaccaggt gcgggaggcg
     3541 cctttccttt gagtggggag gtctcagcca gatacttcag gatagtatag acgtcgcctt
     3601 cttctggaag ggtgagatcg aagtcctgcg tacgaaagcg aatccgggcg ccgcgctcgc
     3661 tgacgtgcca aacgaggaat gcacaacagt ccaccgcttg ttcgaaccat tccagtcttt
     3721 ccggggcgac ttccaggtcg agaaacatct cgatgaggtg ctcctgctcc tgggcaaatt
     3781 cccggacctg caagtcgcct gtatgcgccg tggccttcca gtccacgtgg cgcgcgcttt
     3841 cgaacgcttc gtagggccgg atgcggtaga agtcatgacc gcgcccgcga taatatgcct
     3901 ggatttcgcc ggaaagcgcc tgtaagaggg cctcgaaacc ggattgcgga ccgatggagg
     3961 gatataccag aatctcgcgg cgcagccgga ctcgaatgcg ccgctcggtg aatccgaacg
     4021 gaaaacgcgt ggagaattga aagctgttct cacgatagat tccgcggcgg gtgaagaaga
     4081 cctccacccc ttcttccacc gtggcattac gcggaatgga tggaaagtag accacggatg
     4141 acatcccggt gtcgggagag cccgccaggt ggatggagaa agaaggcatc caggattttg
     4201 agtttcggat tagaatgcgc gcgctggtcc gggcgcgggc cgagatatgt tcgggaagca
     4261 cgaagtccag ttcgagaccg gacagcgata agcggctgac gaatccggag accagcaggg
     4321 tcgataggag cgccgcgaag agcaggaaca gaagattatt agcggaaaca aaggcggcga
     4381 ctccgacaag tgaaatggta aaagtaaata gcagaccgcc cgccgttacc tggtggcgca
     4441 gcgccatttc cagggcgtcc cgaagcgctt tccaaatggg cctgacgctc acggaagtgt
     4501 caccgttgtg aagccagcag gttcacgatc ctgtccgtca ggtcgcgtac agacgccgcg
     4561 ggtacatcgt agtgattggc gaggatggaa aacgccaatt gtcccccgcg cctgccgtca
     4621 acatagccgg agagcgcgct gacgtgactg atagtcccgg ttttggcgcg tacgagtccg
     4681 gcggctgcgg ttccacggaa tctgtgctca agcgtgccat cttctccgcc caccgggaga
     4741 agcctgagcc aggcatcacg ttgcggcgaa tgatacatgt gccggagcag gctaacgaca
     4801 gcggaggggc tgaccagcgt ttttctggaa agcccggagg catcctcgaa gtgataggca
     4861 tccggcagga tcccgatttc cgcaagaaac gcttccaact ccgccagacc cgcgtcgcgg
     4921 ctgccgagat ctctccgcat tcggcccact tcgcgcaaaa cgacctcggc ctgcagattc
     4981 tggctgattt tatcgatgac gcgcaggctc tcgatcagcg ggggagaagt acgcgcggca
     5041 agctcgattc cgcttgccag gaaacgggga ttcgggcctc gggtaaggtc accgatctca
     5101 ccgggcagca ggtgacgggc gacggcctcg cctttgatcc gcacaccgcg ccggattaac
     5161 aggtcccgga acacttccgc cgcatagagg gcgggatcgt cgacggccag atactgaaca
     5221 ctgggccgcg aggaagatgg cagcgtaccc caaaggcgaa ccagcctgga ggcgggtaac
     5281 cggtggaccg aaatctttcg tgtgcccccg gcggtcgtct ccacgcggtt ctgaaacacg
     5341 tagtattcaa aagcaggcga taccagcagg ccggcaagac cgccaggact ggcggatggc
     5401 tggagcgata tacgaatcga attgtcgccg acgatcaagg cgctggcggg tgctccgtat
     5461 tcccagatgg catcgtccac ggtccagccg tccggataag gttcccagac atacgcggtg
     5521 tcgtcgccaa cgacatcccc ttcgatacgg ttcacaccgg cagcgacgac ctgatcggcc
     5581 agcgaggcca gaggagcgag cggatctcct tcgacaggtc cggggcgata cggcagagac
     5641 cgggccgaaa gagtcatgtc tccgccgccg atcaggagaa gatcggaacg gaggattcca
     5701 tccgatgcgg gctgcaccgt cgacacgact cgtgtgtaga agcggtgatc cggcccgagt
     5761 ttttgcagtg ccaaggcagt tgagaagagc ttggtgttgg aagcaggagt gaaaagctta
     5821 tcctggttgc ggttgtacag caatcgacct gtacggaggt ctagtacttg aattccccag
     5881 taggatcttt ctgctaccgg agaattttta agtaatactt caattcgggc gcttagcggc
     5941 ggcccggcag ccaccagagt ttgagcgaca agcagtagag cgacgcgtcc cattgatcga
     6001 tttaccccag acctgtaggt ttatcggcag tttctttaat cctgatttca gcgcctcccg
     6061 acaacttggc ggtcagccag atcatcagca caagcacaaa gacggcaaat gtacctaaca
     6121 gggggagcag cataaccact ctgatgcccc aaaactccgc gaaataccct agagtaccgg
     6181 gcgccagcag cccgccgacg gaggctacgg cagaaattcc gttgaacaaa ccaggatggt
     6241 agttcggaaa tcgtagaccg atcttttcca ccagcaaggg gtagacgacg gcgaagccga
     6301 gccccagtaa aacgattgcc gttatggcgc cgaaccgatt attggtaaag ctcagaatca
     6361 gacagccaaa aaccgcgcag gcggcgctac cggccagcag ccacccacgc ttcattcggg
     6421 gaattacgaa atgaacggtg agccggccca ccagcagagt aagccaatac aaagagagga
     6481 gcaggaggga agtttccggg ct
//
LOCUS       DASTMK010000049         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_578823, whole genome shotgun sequence.
ACCESSION   DASTMK010000049 DASTMK010000000
VERSION     DASTMK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578823"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1455
                     /locus_tag="VFQ79_02105"
     CDS             184..1455
                     /locus_tag="VFQ79_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0138468.1"
                     /translation="MTTHRRRFLSTALGGGLASQPLRLWGSPPSPEVDARYRQLGAVL
                     QQPVLKKQHFRAPVVIETVELLRLDNSFLCRVRSRDGAEGISVAHSGMSTLFPIFLRN
                     LQPFFPGKDARELDLILEKIYIYNFNFRYGGIALGLPLATIEFAILDMLGRIAQKPVG
                     QLIGEIHNPEVGVYVATEWREKPVEESIRLIQEAVAAYDTNALKIKVGGLMFMTTDMY
                     AAGPPGRTEKMIPLVRKTFGDSMAIYADSNSFYSVPEAIRVGRLLEEYKYGYFEEPVM
                     FDHLEEIKQVSDTLTIPIANGEQDYSFYGFRWLLANNGIDIVQPDNYYFGGLIRSMKV
                     ARMAAAVGKTIVPHMSGGGLGYLYNIHFVSAVPNAGAHHEFKGLRTNVRFECKTSPLK
                     VAGGKMKVPTGPGFGVDFDPDWVKKHQVVKL"
     gene            complement(1462..3519)
                     /locus_tag="VFQ79_02110"
     CDS             complement(1462..3519)
                     /locus_tag="VFQ79_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HEU0138469.1"
                     /translation="MASLRTITHGSAAAILIVLVLVAQGPPSVKVEGKGIRLEFDRSL
                     QSQVIAVLDGKETAMGPVQASETITVNGQELGDFPLAHSNSQVVKDDLGAGRQFTITG
                     VSGAIRKTVAATVYDAFPQMVFLKVRYANEGKSDLLVSGWTNHRYVIGAGPLKGGAEP
                     AFWSYQPGSYKERPDWVLPLKPGFKQENYLGMNATDYGGGTPVVDVWRRDLGLAVGHI
                     ETTAKLVSLPVTMPDDGRATLELSYRAPQTLKPGTSLETFRSFVAVHRGDYFHALKAY
                     SAVMQKRGLKLAAAPPSAFEPIWCAWGYGRGFTPKQVEGALPVVKKLGFGWVGVDDGW
                     QSSDGDWGLVTSKFPNGDRDMRALADKIHAQGFRAQIWWAPLAAKPDSNLVKEHPEQL
                     LLNADGSKQKISYWNDWYLCPSDPAVLEHHKKLVVKMIRDWDFDGLKLDGQHMNGVPA
                     CHNPSHKHARPEESVEGLATFFKMIYDTARSIKPDALVEWCPCGTSFNFYTLPTLNMS
                     VASDPRSSWQVRTKGKTLKALHGDGTAYFGDHVELSDGRQDFASTVGVGGVVGTQFTW
                     PAGSGRNTRLDLTEAKEPVWKKWISIYNDKRLSQGEYRGELYDIGFYRPEAHAIRKAG
                     RMYYAFYAPQYEGRVELRGLERRAYAVTDYENGKALGTIQGPVGQLDVQFKAHLLLEA
                     APE"
     gene            complement(3526..>3993)
                     /locus_tag="VFQ79_02115"
     CDS             complement(3526..>3993)
                     /locus_tag="VFQ79_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138470.1"
                     /translation="DGWIFFGRDRYKRSPAGGEILFPNKEFEKMVASRWATEAKNFGI
                     TFMITEQWPRWTDMDAIRRHGMACGYKFRVTEFAADAKQSRVTVTNTGVAPIYYDAYV
                     AVNGVRARESLKYLQPGETRQFTIESGGTAPDLTIECGRLVPGQRIEFDADLN"
BASE COUNT          813 a         1253 c         1110 g          817 t
ORIGIN      
        1 acctgagacc gcggcgcgtc gacgtgacag gcctgcctaa gtccccctac accctttgcg
       61 cgggggacag ggaccggtcc cggtgcgcgg gcaaatgcgc accagtaaac atcaccgatc
      121 agcgagcaac accgagccgc gcggccatct cctgcatgcc acaatgaaac ccaggagtat
      181 tccgtgacca ctcatcgccg caggtttctc tcgacagctc tgggcggagg gcttgcctcc
      241 caaccactgc gcttgtgggg ttcgccgcca tcgccggaag tagatgcccg ctatcgccag
      301 cttggcgccg ttctgcaaca acccgtgctg aaaaagcagc acttccgtgc accggtggtg
      361 atcgagacgg tcgaactgct ccggctggac aacagttttc tttgcagggt gcgttcccgt
      421 gatggagccg aaggcatctc ggtggcccac agcggcatga gcacactctt ccccatcttc
      481 ctcagaaatc tgcagccgtt ctttccggga aaagacgccc gcgagttgga cctcatcctc
      541 gaaaaaatct atatctacaa tttcaacttc cggtacggcg gtatcgctct gggcctgccc
      601 cttgccacca tcgaattcgc catcctcgac atgctcggcc gcatcgcgca aaaacccgtt
      661 ggccagctca tcggcgagat tcataatccc gaggtcggcg tctacgtcgc caccgaatgg
      721 cgcgaaaaac cggtcgagga atccatccgg ttgatccagg aagccgtcgc cgcttacgac
      781 accaatgcgc tgaagatcaa agttggcggg ctgatgttca tgaccacgga tatgtacgcc
      841 gccggccctc ccggacgaac cgaaaagatg atcccgctcg tgcgcaaaac gttcggagac
      901 agcatggcga tctatgccga ctcgaacagc ttctattcgg ttcccgaagc catccgcgtc
      961 ggcagactgc tcgaagagta caagtacgga tacttcgaag agcccgtgat gttcgatcat
     1021 ctcgaggaga tcaaacaggt ttccgacacg cttaccatac ccattgcaaa cggcgagcag
     1081 gactacagct tctacggatt tcgctggctc ctcgcgaaca acggcatcga tatcgttcag
     1141 cccgataact actatttcgg cggactcatc cggtcgatga aagtggcgcg aatggcggcc
     1201 gcggtgggca aaaccatcgt gccgcatatg tccggcggcg ggctgggata cctttacaac
     1261 atccacttcg tatccgccgt tccgaacgcc ggcgcccatc acgaattcaa aggcctgcga
     1321 accaacgtac ggttcgaatg caagacatcc cctctgaaag tggccggcgg aaagatgaag
     1381 gttcccaccg gccccggatt cggcgtggac ttcgatccgg actgggtgaa gaagcaccag
     1441 gttgtgaaat tgtgaggggc gctattccgg cgcagcttcc aggagcaggt gcgccttgaa
     1501 ttgcacatcc aattggccca ccggaccctg gatcgttccc agcgcctttc cattctcgta
     1561 atcggtcact gcgtaggcac gccgctcgag gccgcgcagt tcgaccctgc cttcgtattg
     1621 cggagcgtag aatgcgtagt acatacggcc ggccttgcgg atggcgtggg cttcagggcg
     1681 gtaaaagccg atgtcatata actcgccgcg gtactcgcct tgcgaaagcc gcttgtcgtt
     1741 gtagatcgaa atccacttct tccaaaccgg ctccttcgct tccgtcagat ccagacgcgt
     1801 gttccttccc gagcccgccg gccaggtgaa ctgcgtgccc actacgccgc ccactccgac
     1861 cgtggaggca aaatcctgcc tgccatcgct cagctccacg tgatccccaa agtaagcggt
     1921 gccgtcgcca tgcagcgcct tcaaggtctt gccttttgtg cgcacttgcc aggaactgcg
     1981 aggatcggag gccacgctca tgttgagagt cggcagcgta tagaagttga acgaggtacc
     2041 gcacgggcac cattcgacca atgcatccgg cttgatgctg cgcgccgtgt cgtaaatcat
     2101 cttgaagaag gtagccaggc cttcgacgga ttcctccgga cgcgcgtgct tatgcgacgg
     2161 attatgacag gccggcacgc cgttcatatg ctggccatcc agcttcagac cgtcgaaatc
     2221 ccaatcccgg atcattttta cgacgagctt tttgtggtgt tccagaacgg cgggatcgct
     2281 ggggcagaga taccagtcgt tccagtagga gatcttctgc ttggacccgt cggcattcaa
     2341 tagcagttgt tccgggtgct cctttaccag attggaatcg ggcttggcgg caagaggcgc
     2401 ccaccagatt tgagcgcgaa acccctgggc atgaatcttg tccgcaaggg ccctcatgtc
     2461 tcgatcgccg ttcggaaatt tcgacgttac cagcccccag tctccatccg acgattgcca
     2521 gccgtcgtcg acgccaaccc acccgaagcc cagtttcttg accaccggca gggcgccttc
     2581 cacctgctta ggcgtgaagc cgcgaccgta gccccaggcg caccagatcg gctcgaaggc
     2641 gctcggagga gcggcggcga gtttcaagcc tcgcttctgc attacggcac tataggcctt
     2701 cagagcatga aagtagtcgc ctcgatggac tgccacaaag cttcggaagg tctcaagcga
     2761 tgtgcctggt ttgagcgtct gcggagcccg gtaggacaac tccagcgtcg cgcgtccgtc
     2821 gtccggcatg gtaaccggca gcgacaccag ttttgcagtt gtctcgatgt gccccactgc
     2881 gagtcccagg tcgcgccgcc acacgtctac aaccggagtt cccccgccgt aatcggtggc
     2941 attcatgcca aggtagttct cctgtttgaa acccggcttg agaggcaaca cccaatccgg
     3001 ccgctctttg taggatccag gctgataaga ccagaacgcc ggttccgcac cgcccttcag
     3061 cggcccggcg ccgataacgt accggtggtt ggtccagccg gataccagca gatcggactt
     3121 cccctcattg gcatagcgca ctttgagaaa aaccatttgc ggaaaggcat cgtacactgt
     3181 cgccgccaca gtcttgcgga tggcccccga tactcccgtt atcgtgaact gccggccggc
     3241 tccaagatcg tctttgacca cctggctgtt gctgtgcgca agggggaagt cgcccaattc
     3301 ctggccattc acggttatcg tctcggatgc ttgaacgggc cccatcgcgg tttccttccc
     3361 gtccaatacg gcaataacct ggctctggag cgaccggtcg aattccaggc gaatgccctt
     3421 gccttcgact ttgacggaag gcggtccttg cgcgaccagt accaggacaa tcagaatcgc
     3481 ggccgccgag ccgtgagtga tggttcgtag ggaagccatg gttacctagt ttaaatctgc
     3541 gtcgaactcg atgcgctgtc ccggaacgag ccggccgcat tcgatggtca ggtcaggagc
     3601 ggtgccgccc gattcaatag tgaactgccg ggtttctccg ggctggagat acttcagcga
     3661 ttcgcgggcg cggactccat tcaccgctac ataggcgtca tagtaaatgg gggccactcc
     3721 ggtattggtg accgtcacgc gcgactgctt cgcgtccgcc gcgaactcgg ttacccggaa
     3781 cttgtagccg caggccatcc cgtgccggcg gatggcatcc atatccgtcc atcgcggcca
     3841 ctgttccgta atcataaaag taattccgaa gtttttcgcc tcggttgccc aacgggacgc
     3901 caccatcttc tcgaactctt tattggggaa aagaatttcg ccgccggccg gcgatcgctt
     3961 ataacgatcg cggccgaaga agatccaacc gtc
//
LOCUS       DASTMK010000050        15782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_450718, whole genome shotgun sequence.
ACCESSION   DASTMK010000050 DASTMK010000000
VERSION     DASTMK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15782
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450718"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(720..1202)
                     /locus_tag="VFQ79_02120"
     CDS             complement(720..1202)
                     /locus_tag="VFQ79_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0138471.1"
                     /translation="MLRQGINLTVNPSEETIRLGPLTVRFLLTRDNSAGSIAAFEVLV
                     PPGQRLAAPAHSHDHFEETIYGIAGALAWTVDGKQIEVGPGQALCIPRGAIHRFDNIG
                     SMDAMALCVITPAAIGPEFFRESAEVINTAAGGPPDRAEMSEIMRRHGLTPAPPPGQA
                     "
     gene            1306..1725
                     /locus_tag="VFQ79_02125"
     CDS             1306..1725
                     /locus_tag="VFQ79_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0138472.1"
                     /translation="MLIGALLRVPAQAIQRRIIKELNAAGFEELSMPHMAVLQFPGPD
                     GSRPGTLAGRAGMSKQAMNRLLGSLEDFGYLVRNDAPGKGRGRVVHFTKRGYAAYAKV
                     HDILRDIEQEWSAELGAKDFAQLKALLARVWDSRLID"
     gene            2333..2578
                     /locus_tag="VFQ79_02130"
     CDS             2333..2578
                     /locus_tag="VFQ79_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138473.1"
                     /translation="MKPRPGVVCSLLVICANAGFAQGPEDRRWRFSSQAVFKNQHVWR
                     GFVESREPTTIAEVTFAYGGLEMNLENVIRPIWEFSG"
     gene            2594..3826
                     /locus_tag="VFQ79_02135"
     CDS             2594..3826
                     /locus_tag="VFQ79_02135"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU0138474.1"
                     /translation="MQDSELYAQILGIRRPWRVDRVELKLGQGEVHVHLLHESGVEWQ
                     CPECGASCSLYDHQPERRWRHLDTCQYQTILHAETPRANCSEHGARAVKLPWAEPSSR
                     FTALFERLAIDWLKATSQSAVAEQLRLSWDEVHAIQERAVERGLKRRKAESVERLGID
                     EKSFTRGHRYFTLVNDLDQGRVLFVGENRTEESLEPFWAGLTAEQTTAVRAVAMDMWD
                     PYVRSTRKHLPEADGKIVFDKFHIAKHLSEAVDQVRRRENKQLKAAGDDRLKGTRYDW
                     LRHPARMEPEDRKQFRALRDSNLKTARAWALKEAMMAFFGYHYERPARKHFRWWCNWA
                     VRSRLKPMIEKAGMLKRRFENIVTYLRHRITNAASESINSKIQWVKYTARGFRSKKNF
                     VTAIYFHCGGLDLMPSTH"
     gene            3888..4337
                     /locus_tag="VFQ79_02140"
     CDS             3888..4337
                     /locus_tag="VFQ79_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138475.1"
                     /translation="MIHPRYTRQFGRFKTSVGWRLKRFPSGRGQAASGTNEGYAQISF
                     LGTLNPTYTIVRDVQLGSGWYQHLQLLHPVRLSRRVTLEGMAGIGLNQHYFIKQTTVS
                     DVSYGLAARISVTSSFGFAPFYNYIFGHRSIFGRRHVIGVSVVLGSE"
     gene            4438..5736
                     /locus_tag="VFQ79_02145"
     CDS             4438..5736
                     /locus_tag="VFQ79_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138476.1"
                     /translation="MSFRRQWITISYLTLAALPAAAAAPVWKPARQAPPLPAREGRAV
                     PVSTVPALRQAMENARPGDTILVADGTYMLEQPLLFEGKQHVTLRSASGDPAKATLRG
                     AGGWRGPADDYRSIGNEDLLVIQRSEDIIIAHLTFAEVRHYGIKIGIDADRIPPNPSN
                     IHIYDCRFRDIATRAIKGVAMADRKPVVGGSVRFCDFENTRIPDASWGWAIYEGNYVS
                     AIDMMYLKDWTFSDNVFRNFKGATGGARGAIFIWNQSRNITVERNMFIGCDRSIAFGN
                     PSKPTGYEPGTLHVYDGWVRNNFIVMDSIPKGGGSKGIEIVWTDNVRVYHNTIRAADP
                     RYRAIHYFENIHRLHLANNLVRGRIEGEGDVRAEGNVAGGLEGYFVNPVEGDLHLTGR
                     AADALGKGVALAAVTDDFDGEKRGSPPDVGADERSPRVTR"
     gene            complement(5748..6353)
                     /locus_tag="VFQ79_02150"
     CDS             complement(5748..6353)
                     /locus_tag="VFQ79_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138477.1"
                     /translation="MNIRRNGLLLLIALAGVHAADNDKNRFSPKPAASYPFHQTNGKV
                     TIAAVPYETDEQARTAFDKRNPYKYGVLPVLLVIQNDSDTALRLDGMRAEYTTPDGSR
                     IEATPAADVRFISGPQKPNMAPNPLPIPRRAKKNPLDMWEIEGRAFSAKMLPAGEAAH
                     GFVYFQTRHSPNSRIYITGLSEAPTGKELFFFDIPFTADKR"
     gene            6352..8706
                     /gene="lpxK"
                     /locus_tag="VFQ79_02155"
     CDS             6352..8706
                     /gene="lpxK"
                     /locus_tag="VFQ79_02155"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU0138478.1"
                     /translation="MYILYRVLQFTTLPLFLLYVLGRGLRDVRYFARLADRFGFLPDS
                     YKRTVAGAVWLHAVSVGEVLSSVELLRRLRAALPEAPLFVSCSTVAGRASAEGKLKGL
                     VDGIFYAPYDLVFAVRATLRAIRPMVVAVMETEIWPNLYREVSRSGAGLVIINGRISD
                     RAFPRYRRLAWFFRRVLSWPDVVLVQSERDRERYLEIGAPASSVRVGGNLKYDFDPRR
                     AGLADDLRAFLEEAAPERTWIAASTMPPRNSGDVDEDDVVIGAFAELAHSHPGLLLIL
                     VPRKPERFAVVAAELEAKGIRFIRRTTLQDRGRFQLPGVLLLDSIGELGGLFPVADVV
                     FMGGTLAERGGHNILEPAFSGRAIIIGPHMENFAAIAEKFRAGNALHAICSSGELAGA
                     VDYLLSDEKRRAELGGRALALAESERGATDIAVSTILDLYARAVPSRVPNLASGLFLK
                     PLARGWAVGSARAHRKSLARKRRLETPVVSVGGISMGGTGKTPFVLWLAGRMKEAGFR
                     PAILTRGYRRKCPNEPTVIEPGADAPVALTGDEAQILCRSAVAYVGIGSERFEVGRII
                     EQKFRPGLFVLDDGFQHWRLERQLDIVLVDALDPFAGGHLFPAGRLRESPSALGRADV
                     IVLTRTTPGRTYEGIETELRRFNPHAPVYHARVRPLEWKDAETDETWAPGSLPFRIAG
                     AFCGLGNPASFWRTLDTLGYRTAFQFTFPDHHQYSIDGLQKMATRAKETGAGVLLTTE
                     KDLINLPDKAAALVKPARICWLKVRTEIEEEDALLEVVMQSLKR"
     gene            8760..9002
                     /locus_tag="VFQ79_02160"
     CDS             8760..9002
                     /locus_tag="VFQ79_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138479.1"
                     /translation="MSDPVYLELVDFVAGGTTPEDVANFHPSPEAQKRLSELIERERE
                     SRLTPEESAELTHFLELEHILRMAKAKARLILANRS"
     gene            8999..9406
                     /locus_tag="VFQ79_02165"
     CDS             8999..9406
                     /locus_tag="VFQ79_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU0138480.1"
                     /translation="MRVSPELRSKVAQRSGLRCEYRLVHEDDAAFAHEVDHVLSRQHG
                     GETATENLAYACMICNRFKGTNVGSVDALGGIVRLFNPRTDEWGKHFRLEGAVIQPLT
                     AIGEATARLLRLNTAERVTERTVLQRLGRYPRE"
     gene            9524..10030
                     /locus_tag="VFQ79_02170"
     CDS             9524..10030
                     /locus_tag="VFQ79_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEU0138481.1"
                     /translation="MRTRELPRRTPTGYDRPLKPEVLPKTDDPLMEWLVWLMDQSVRT
                     GRFSFGLDGILGLVPGFGDLAGGMVSAFIVYRAARAGVPKAAVARMMANVAVDSALGA
                     VPFIGDLFDFLYKANTKNLKIYQEAMAGTRDTRRDWGFVLLVIVGVLALLAIPLILIG
                     VVIAALLR"
     gene            10145..12433
                     /locus_tag="VFQ79_02175"
     CDS             10145..12433
                     /locus_tag="VFQ79_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0138482.1"
                     /translation="MKEASKVRKIAFVGDYLPRKCGIATFTSDLLTAVAAAHPHCQCF
                     SVSVNDVKGRYEYPEVVRFEIDEQDLSSYLRAADFLNISNADIVCLQHEFGIFGGQAG
                     GHILALLRELRMPVVTTLHTVLSEPRADQKRVMQELISLSTRLVVMVDRGRRMLQEIY
                     HAPPSKIDVIPHGIPDVGFVDPTFFKDLFGVEGKIVLLTFGLLSPNKGIEYVLNAMPR
                     ILAEFPETVYIVLGATHPNELREHGEAYRYSLEVLAKKNNIEKNVIFYNSFVELENLK
                     EFIGAADFYITPYINEAQITSGTLSYAFGCGKAVVSTPYWHAQELLADDRGVLVPFRD
                     SDAIARELLGLLRDDTRRHAMRKNAYRLGREMVWSNVAHTYMHSFELSRTEGAAHPQK
                     SLVTKTLDRKARELPEVNLSHLSRLTDSTGIFQHAVFSVPNFSEGYCTDDNARAFIFT
                     VLLSELGVDPEKVRTLSTTYAAFLRHAFDPSTNRFHNHMSFDRRWLDERGSEDCHARA
                     LWALGVGVGRSPYRSFQVMAGQLFEQALPALLDFRSPRAWAFGILGIHEYLRLLSGDS
                     LAAQARETLTSRLMDLFDRTAQPDWRWFEERLTYDNAKLPHALILSGRATKQEAVLER
                     GLEALRWLSELQVSERGYFQCIGSNGFYSRGGIRASFDQQPIEAHAMVSACLDAFRVT
                     ADSWWYNQAQRAFDWFMGGNDLGLELYSSKTGGCRDGLHVDRVNRNQGAESTLAFLLS
                     LAEMRLAQNMLTSFKEPFALES"
     gene            12472..13935
                     /locus_tag="VFQ79_02180"
     CDS             12472..13935
                     /locus_tag="VFQ79_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEU0138483.1"
                     /translation="MANNSLHVKRTSIILRPDPSRVLLRPFSPGDAQRRGEIISRILS
                     IPEEEVCPLLDEVSVEFSSRHSEIRQLFLARFEQIREQLWTDEELPEERKLLIGSYFM
                     AEYSLESAALFNPSIVPHPDQADLPDGALRFIISLRAVGEGHISSLTFRTGILYADNH
                     IEVPPAAGLLTEPRQIPNALYEKRLFVKKLAEVGLTCDFTRRVMKRLKEQFTLDELRE
                     ALQGEQFRLPDGMSQEDQSASQGIWMLARSNFEVQFQPEQRLSERILFPATPAQRNGI
                     EDARFVRVEEDDGTQTYYATFTAFDGRVIMPQLVETSDFLRFRFITLNGPAAQNKGLA
                     LFPRKINGLYAMLSRQDNENIFLMLSDNVHFWYERTLLMEPKFPWELIQIGNCGSPIE
                     TEAGWLVLSHGVGPLRRYCIGAFLLDRDDPTQVIGRLREPLLEPVENEREGYVPNVVY
                     TCGALLHNGELIIPYGLADYATGFATVPLDDVLAAMH"
     gene            14069..15088
                     /locus_tag="VFQ79_02185"
     CDS             14069..15088
                     /locus_tag="VFQ79_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0138484.1"
                     /translation="MAVLSPVAWRTPPRQYGAWETVASNITEGLVARGWDVTLFATAD
                     SVTRARLHAVVERGYEEDPAVDPKVAEYLHIPEVFERAADFDLIHSHYDFMALTYTRL
                     VRTPVLTTIHGFSSPQILSIYRKYGNGYFVSISDADRAPGLNYLATVYNGIDLSLYPF
                     QPAGGDDLIFLGRIHPDKGVHLAVEVARRAGLPLIIAGIIQDQAYFREQVEPYLDGEK
                     IRYIGPVDVPGKNELFARARALLHLNTIPERFGLVLAEANAAGVPVIAMDLGSCREVI
                     EDGQTGFLVNGVEEAVQSLERLPEIDRGACRRRVEQRFSIDTMVEGYERVYRMIFELE
                     AAKRL"
     gene            15085..>15782
                     /locus_tag="VFQ79_02190"
     CDS             15085..>15782
                     /locus_tag="VFQ79_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEU0138485.1"
                     /translation="MKADVVRRYGYNPILTKADIPYAVETVHNAAVVKHGTEYIMLFR
                     SHLRTGRSIIGLARSPDGFHFTADPEPFLVPARDGLFAGYEEFGVEDPRVTQLEEEYV
                     ITYSAYSRNGVRIALAKTKDFRQVERVSLITQADYRNVVIFPEKFDGLYARLDRPHSE
                     IAPWSIWISYSPDLRFWGESQLIMRPVPYHWDEMKIGPGAPPIRTDKGWLSIYHGVFE
                     TMDGSVYRLGVALH"
BASE COUNT         3111 a         4548 c         4872 g         3251 t
ORIGIN      
        1 tccagccgca atcgcatcaa cccgcgaggt gtgaagcgaa agatgagcaa ctatccgttg
       61 cggccgcgaa tgccgcagtc gacaatcaaa actgacttct ccgcatctat caaaatcatt
      121 aagtgaacag tattggggct agcaccggga ggtgacgtcc tcgcggccaa taagcgggtc
      181 acgagcggct tgagttcgta cttacgatct ttgacgttca tactggatgc cgctagctta
      241 tcgcctctac ggcggtagca cggttcggta tcgttggacc gccacgacga cctctggctt
      301 cttttcagga agttcacgcc atcctcaagc tgcctaaccg cgagtgactt cattctggag
      361 agcacgcaac ccggcggaaa cggatcgccg gaagatcgaa ctggctgcgg acaagttgcc
      421 cgacgcggat ggtgccagtc atgtcctacc tgccaccaga tagtattggg tatggtgagg
      481 gttcctgcaa aaggaggtat cggccggaac acgggccaac cggaacgcgg gagatagtca
      541 agttgaggta acgcccgttg ctccctgttg caaaccggtc cgagccgctg ggcattatca
      601 atcagcggaa ggccgatttc tcgctcatct gaattgattc cgagtggccg gccacttgcc
      661 cgaagagctg aagaaccggc tacacctaag ctatagaagg tgccttctgc ccaaagactc
      721 tacgcctgac ccggaggcgg ggcaggcgtg agaccgtggc ggcgcatgat ctcgctcatc
      781 tccgcccggt ccggcggccc gccggcggcc gtgttgatca cttcggcaga ctcacggaaa
      841 aactccggac ctatcgccgc tggtgtgatc acgcagagcg ccattgcgtc catactcccg
      901 atattatcga accggtgaat ggctcctcgc ggaatgcaaa gcgcttgccc tggtccgacc
      961 tcgatttgtt ttccatcgac agtccaggct agcgctccgg cgataccgta aatggtctcc
     1021 tcgaaatggt cgtggctatg cgccggcgcc gccaggcgct gcccgggcgg gacaagaacc
     1081 tcgaaggccg caatgctgcc agccgagttg tcccgcgtga gcaggaagcg cactgtgagc
     1141 ggccccagcc ggatggtctc ctcggaagga ttcaccgtaa gattgatccc ctggcgtagc
     1201 atgtggtctc cttttggtaa actaagttta ccagataata gtaaactcta tttacccggt
     1261 atgtcaatgc cctcatcgca aaagaattcg ccggcagtta aagatatgct gatcggcgca
     1321 ctgctgcgtg ttccggctca ggccatccaa cgccgcatca tcaaagaact caatgccgcg
     1381 ggttttgagg agctaagcat gcctcatatg gcagtgcttc agtttcccgg tccagatggg
     1441 tctcgaccag gcactcttgc cgggcgcgcc ggcatgagca agcaggcgat gaaccggttg
     1501 ctcggcagcc tcgaagattt cggatacctc gtccggaacg acgcacctgg taagggccgg
     1561 gggcgagttg tgcattttac caagcgcgga tacgccgcat acgcaaaggt ccacgacata
     1621 ctccgcgaca tcgagcagga atggagcgcc gaactggggg cgaaggattt cgctcaactc
     1681 aaggccctac tcgcccgcgt ctgggatagc aggttgatcg attagtcgtg ccggtaccca
     1741 tctgagtgct ccgctctttc cggaggcatg ctgtcaccag ttggcaaaca gtgcatctgt
     1801 gcagttagcg tcatgcagca cggtgttcag tctgaggaag gagcacacgg caaattcagg
     1861 gccgtaacgc tttgggtagc agtcgtccaa gtcgccctga cgttactgag cgccgccgga
     1921 cctccaaaga ttctctgctt tggcaccgac gggcattgcg aattcgagca tgcatacgcg
     1981 aactgttgcc ttcatggcga agcctgcttg gatagcgccg gtgaatcgac tctcgctcaa
     2041 taccatgctt gcgtcgatgt ccttctatcc gcattgctgc gctccccaaa ccaggtgcca
     2101 tcgcccgacg ccgactgcat ggaacaatta gcagtttttc catccgcaat cgatgcgggg
     2161 gcaaagccct tcacgttcgc ctgcgaagcg gacgtgggct ttgcttccca tctctccaac
     2221 tcgttgcctg ttctcctctg ctgatccaac gcttgtctcg ccggattcac gacctgtcat
     2281 tttcgtgctg acaggtttgc ctggatgttc gagatcaaag gagacgtttg atatgaaacc
     2341 aagaccggga gtggtctgca gtttactggt gatctgtgca aacgcggggt ttgcacaggg
     2401 ccctgaagac aggcgttggc gcttctccag tcaggccgta ttcaagaatc agcacgtttg
     2461 gcgcggcttc gtcgaaagtc gtgagccaac cacgattgcg gaagtgacct tcgcctatgg
     2521 aggactcgag atgaacctgg agaacgtaat caggcccatc tgggaattca gtgggtagcg
     2581 tgataatggg cgggtgcagg acagtgaact ttacgcccag atcttgggca tccgccgtcc
     2641 atggcgagtg gatcgggtag aactgaaatt gggccagggc gaggtgcatg tgcacctgct
     2701 tcatgaaagc ggtgtcgagt ggcaatgccc ggaatgcggc gcctcatgct ctttgtacga
     2761 tcatcaaccg gagcggcgct ggcgccactt ggacacgtgc caatatcaaa ccattctgca
     2821 cgccgaaacg ccgcgcgcca actgtagcga acacggagcg cgagccgtca aattgccgtg
     2881 ggcggaacca tcgagccggt tcacggctct gtttgaacgg ctggccatcg actggctgaa
     2941 agccaccagc caaagcgcgg tagccgaaca actgcggctg agttgggacg aagtgcatgc
     3001 gattcaggag cgggcggtcg agcgtggatt gaagcggcgc aaggcagagt ccgtcgaacg
     3061 gctggggatc gacgagaaat cgttcacgcg gggacatcgc tacttcactc tggtgaatga
     3121 tctggatcag gggcgcgtgc tgttcgtagg ggagaatcgg acagaagaga gcctggagcc
     3181 attctgggcg ggtctgacgg ccgagcaaac aacggcggtc cgcgcggtgg cgatggacat
     3241 gtgggatccc tacgtccgct cgacacgcaa gcacttgccg gaagcggatg gcaagatcgt
     3301 cttcgataaa tttcacattg ccaaacacct gtcggaagcg gtggatcagg tacgccgccg
     3361 cgagaacaaa caactgaaag ccgccggaga tgatcgattg aaggggactc gttatgattg
     3421 gctacgccat ccggcgagga tggaacccga ggatcgcaag cagttccggg cgttacggga
     3481 tagcaacctg aaaacggcac gcgcctgggc gctaaaagaa gccatgatgg ccttctttgg
     3541 gtatcactat gagcggccgg cgcgaaagca ctttcgctgg tggtgcaact gggcggtgcg
     3601 cagccggttg aagccgatga tcgagaaagc aggaatgctc aagcggcggt tcgaaaatat
     3661 cgtcacctac ctgcgccacc gcatcacgaa cgccgccagc gagtcgatca actccaagat
     3721 ccagtgggtg aagtatacgg cccgcggctt tcgtagcaag aagaacttcg tcaccgccat
     3781 ttacttccac tgtggcggcc tcgatctcat gccatcaacc cactaaaaag ccggaatgcc
     3841 ctgtaatctc gggtcatccg aagttcgcgg gatactgggc ggaatcggtg attcatccgc
     3901 gctatacccg tcagttcggc agatttaaga ccagcgtcgg atggcggctt aagcgttttc
     3961 cgtcgggccg tgggcaggcc gctagtggca ccaatgaagg ctatgcccaa atttcatttc
     4021 tcgggactct taatccgacg tatacgattg tgcgggatgt gcagttgggc agcggatggt
     4081 atcagcatct tcaactgctt cacccggttc gcttgagccg gcgcgtcact ctcgaaggga
     4141 tggctggcat cggcctgaat cagcattact tcatcaaaca gactacggta agcgacgtga
     4201 gctacgggct cgcagcccgg atttccgtca cttccagttt cggcttcgcg ccattctaca
     4261 actacatctt cggccaccgt tcgatattcg gaaggcgtca cgttattgga gtgtcagtgg
     4321 tgctcggcag cgaataagag acgtttgctg atttgactgt gaacgccgga cgatcagttc
     4381 ctccctgacg gcgaagagtt aaccactcct tctgatacac tgccgggagg ttcttatatg
     4441 tcattccgaa gacagtggat aacgatctca tacctgacgc ttgccgccct tccggcggcc
     4501 gctgccgctc ccgtgtggaa acccgcacgc caggctcctc cgctgccggc tcgcgaggga
     4561 cgggcggtgc cggtgagcac ggtcccggcg ctccggcagg cgatggagaa cgcccgtccc
     4621 ggcgacacca ttctggtcgc cgatggcacc tacatgctgg agcaaccgct gttgttcgaa
     4681 ggtaagcagc acgtcactct gcgcagcgcc tccggcgatc ccgcgaaagc gactctgcgg
     4741 ggcgccggcg gctggcgggg tccggccgac gactatcgga gcatcgggaa cgaggacctg
     4801 ctcgtcattc aaaggtccga agacatcatc attgcccacc tcactttcgc cgaggtgcgt
     4861 cattatggca tcaagatcgg catcgacgcc gatcgcattc cgcccaaccc cagtaacatc
     4921 cacatctacg actgccgttt ccgcgatatc gccacccgcg ccatcaaggg cgtcgccatg
     4981 gcggaccgca agcccgtcgt gggtggctcg gtgcgctttt gtgacttcga gaacacgcgg
     5041 atcccagacg cctcctgggg gtgggcgata tacgaaggga actacgtctc ggccatcgac
     5101 atgatgtacc tgaaggactg gaccttcagc gacaacgtct tccgcaactt caaaggcgct
     5161 accggcggtg cgcggggagc gatcttcatc tggaaccaga gccggaacat cacggtggag
     5221 cgcaatatgt tcatcggctg tgaccgctcc atcgctttcg gcaatccatc caagcccacc
     5281 ggctacgagc ccggcacgct ccacgtatac gacggatggg tccgcaataa cttcattgtg
     5341 atggactcca tccctaaggg aggcgggtcc aaggggatcg agattgtgtg gaccgacaac
     5401 gtgcgggtgt atcacaacac catccgggcg gcagacccgc gttaccgcgc gatccactac
     5461 tttgaaaaca tacatcgcct ccacctggcc aataacctgg tgcgcggccg gatcgaaggc
     5521 gagggcgatg tgcgcgccga ggggaacgtg gcgggcggcc tggagggcta cttcgtcaac
     5581 ccggtagagg gagatctcca tctgaccggg cgagccgcag atgctctggg caaaggtgtg
     5641 gccctggcgg ccgtgaccga cgacttcgac ggtgaaaagc ggggcagccc gcccgatgtt
     5701 ggggccgatg aacggtcacc ccgggtcaca aggtagctgc caattcgcta ccgcttgtcc
     5761 gcagtgaacg ggatatcgaa aaagaacagc tctttgccgg taggggcttc gctgaggccg
     5821 gtgatgtaga tgcgggaatt ggggctgtgg cgcgtctgga aatagacgaa tccgtgtgcc
     5881 gcctcgccgg cgggcagcat cttcgcggag aaggcccggc cctcgatttc ccacatgtcc
     5941 agcgggtttt tcttggcgcg cctcggtata ggcaaaggat tcggcgccat gttgggcttc
     6001 tgcggcccgg agatgaagcg gacgtcggca gcgggcgtgg cctcgatgcg gctcccgtcc
     6061 ggtgtggtgt attccgctcg catcccgtcg agccgcaggg ccgtgtccga atcgttctgg
     6121 atcaccagca ggaccggcaa cacgccatac ttgtaagggt tccgcttatc gaaagccgtc
     6181 cgggcctgtt cgtcggtctc ataaggcact gcggcgatgg ttactttccc gttggtctgg
     6241 tgaaatggat acgaagcggc cggtttcggg ctgaagcggt tcttgtcgtt atccgcggca
     6301 tggacccctg ccagcgcgat caacagaagc aaaccatttc gacggatgtt catgtatatc
     6361 ctttaccggg ttctacagtt cacgacgctg cccctgtttt tactttacgt cctggggcgc
     6421 ggattgcgcg acgtccggta tttcgcccgt ctggccgacc gttttggttt tctcccggac
     6481 tcctacaagc ggacggtggc aggcgccgtc tggttgcacg cggtttccgt gggcgaagtg
     6541 ctgtcctcgg tcgagctgct cagacgtttg cgcgccgctc tcccggaagc gccgctgttt
     6601 gtatcgtgtt ccactgtggc gggacgcgcc agcgccgaag gcaagttgaa gggcctggtg
     6661 gacgggatct tttacgcacc ctacgacctc gtgtttgcgg tgcgcgcaac gctgagagcc
     6721 attcgtccca tggtggttgc cgtcatggaa acggaaatct ggcccaactt ataccgcgag
     6781 gtaagccggt cgggagccgg gctggtcatt ataaatgggc gcatctcgga ccgtgcgttc
     6841 ccaagatacc ggcgcctggc atggttcttt cgccgtgttc tctcctggcc cgatgtcgtc
     6901 ctggtgcaga gcgagcgcga tagggaacgc taccttgaaa tcggcgctcc tgcgtcgagc
     6961 gtccgcgtgg ggggtaacct gaaatacgac ttcgacccgc gccgggccgg ccttgcggac
     7021 gatttgcggg cttttctgga ggaggctgct ccggaaagaa cctggattgc ggcgagcacc
     7081 atgccgccgc gaaacagtgg agacgtggac gaggacgatg tggtcatcgg ggctttcgcc
     7141 gaactcgcgc acagccaccc gggtctgctg ttgatcctgg tgccgcgcaa gccggagcgt
     7201 ttcgcggtcg tggccgccga gctggaagcc aaaggcatcc gtttcatccg ccgcacaacg
     7261 ctccaggatc gcgggcgctt ccagcttccg ggggttctgc tgctcgattc catcggagaa
     7321 ctcggggggt tgtttcccgt ggccgacgtc gtgttcatgg gaggcacgct ggccgagcgc
     7381 ggcgggcaca atatcctgga accggctttc tccggccgcg ccattatcat cggcccgcat
     7441 atggagaact tcgccgcgat tgcggagaag ttccgtgcgg gcaatgctct ccacgccatc
     7501 tgttcgagcg gtgagctggc gggggcggtc gattacctgc tgagcgatga aaagcggcgc
     7561 gcggaactgg gcgggcgcgc acttgcttta gccgaatccg aacgcggcgc caccgatatc
     7621 gctgtctcta cgattctcga tctgtatgcg cgggccgtcc cttcgagagt gcccaatctg
     7681 gcgtccggcc tttttctgaa gccgctggcc cgcgggtggg cggttggcag cgcgcgcgct
     7741 caccgcaaga gcctggctcg caagcggcgc ctggagacgc cggtggtcag tgtgggagga
     7801 atcagcatgg gcggaacggg aaaaactccg ttcgtgctct ggctggccgg gcgcatgaag
     7861 gaagccggat tccgtccagc gatcctgacg cgggggtacc ggcggaaatg cccgaacgaa
     7921 ccgactgtta tcgagcctgg cgccgatgcc ccggtcgctc tcaccggcga cgaggcgcag
     7981 atcctgtgcc gcagcgcggt ggcgtatgtg ggtatcgggt ccgaacgctt tgaagtggga
     8041 cgtatcatcg agcagaagtt ccggcccggg ctcttcgttc tggatgacgg atttcaacac
     8101 tggcgcctcg aaaggcaatt ggatattgtc ctggtcgacg cgctcgaccc cttcgcgggc
     8161 ggccacctgt ttcccgccgg aaggttgcgc gaatctcctt cggccctggg tcgcgccgac
     8221 gtgattgtgc tgaccagaac cacaccgggg aggacgtacg aggggatcga aaccgaattg
     8281 aggcggttca atccgcatgc accggtctac catgcgcgcg tgcggcctct tgaatggaag
     8341 gacgcagaaa cggatgaaac ctgggcgccc ggaagtcttc cgttccgcat cgcgggggcg
     8401 ttctgcgggt tggggaatcc cgcttctttc tggaggacgc tcgacaccct cggctaccgc
     8461 acggcgttcc agttcacgtt tccggaccat catcagtaca gcatcgacgg gctgcaaaag
     8521 atggcgacgc gcgcaaaaga gaccggcgcc ggcgtgctgc ttacaaccga aaaggatctc
     8581 atcaacctgc cggacaaagc cgccgcgctg gtgaaaccgg cgcgcatctg ctggttgaag
     8641 gtccggacgg agatcgaaga agaggacgcg ctgctcgagg tggtgatgca gagtctgaag
     8701 agatgacgcc gcgggaacgg cttggggtat cctcaaagaa ggcagcgagt ggcaggaacg
     8761 tgagtgatcc ggtctatctc gaactggtgg atttcgtcgc tggcggcacg acgccagagg
     8821 acgttgcgaa ctttcatccg tcacccgagg cacagaaacg tctcagtgag ttgatcgagc
     8881 gggaacggga gtcccggctc acgcccgagg aaagcgctga gctcacgcac ttcctcgagc
     8941 tcgagcatat ccttcgtatg gccaaggcaa aggcgcggct gattctggcg aacaggtctt
     9001 gagagtcagc ccggaactcc ggtcgaaggt ggcccagcgc tcggggttgc gatgtgagta
     9061 ccgcttggtt cacgaggacg atgccgcctt tgcccacgaa gtcgatcacg ttttgagccg
     9121 ccaacacggc ggcgagacgg ctactgaaaa ccttgcctac gcgtgcatga tctgcaatcg
     9181 gttcaaagga accaacgtcg gatcggtgga tgcactcggc ggcatcgttc gactgttcaa
     9241 cccgcggaca gacgagtggg ggaagcactt ccggctggag ggcgccgtca tacaaccgct
     9301 gactgcgata ggggaagcta ccgcgaggtt gctccggcta aacaccgcgg agcgtgtcac
     9361 agagcggact gtgcttcaac gtctcgggcg atacccgcgc gagtagcaga tagcgactgc
     9421 tgggaacgcc taaagcgtct cgttctcgtg ttttcgcggt ttctcaaaac caggatgccg
     9481 gtttatgcgt cacatagaag atgcaaaggg cgccgcagga atcatgagaa ccagagagct
     9541 tccccgccgg accccgaccg gctacgacag gcctttgaaa ccggaggtgt tgcccaaaac
     9601 ggacgatccg ctcatggagt ggctggtctg gctgatggat cagtcggtcc gcaccggacg
     9661 cttctctttc gggctggacg gcattctcgg cctggttccg ggtttcggcg atctggccgg
     9721 aggcatggtt tccgccttca tcgtctatcg tgcggcccgg gccggtgtgc cgaaggccgc
     9781 cgtggcccgt atgatggcga acgtggcggt ggattcggcg ctgggcgcag tccccttcat
     9841 tggcgatctg ttcgacttcc tatataaggc gaacaccaaa aacctgaaga tctaccagga
     9901 ggcgatggcc ggaaccaggg atacccgccg cgattggggc ttcgtgcttc tcgtgattgt
     9961 gggtgtgctc gcgctgctgg cgataccgct aatcctgatc ggagtcgtga tcgccgctct
    10021 cctccgctga cgcggatcgt cccggctgcc gcgggcggct acaattagta agaggacccc
    10081 aagtgcctta aattccaatt ggaaggagat acagaattct tatcggaaat aaaggaacgg
    10141 gtctatgaaa gaggcttcga aagttcgcaa gatcgccttc gtgggcgact atttgccacg
    10201 caaatgcggc atcgccacct ttacgtccga tctgctgacc gcggttgcgg ccgcgcatcc
    10261 ccattgccag tgtttttcgg tgtcggtcaa cgacgtcaaa ggccgctacg agtacccgga
    10321 ggtggttcgc ttcgagattg acgaacagga cctgtcttct tatctgcgcg ccgcggactt
    10381 cctgaacatc agcaacgccg acatcgtgtg cctgcaacat gagttcggta ttttcggcgg
    10441 gcaggcgggg ggacacattc tagcgctgct gcgggaattg cgaatgcccg tggtgaccac
    10501 actccacacg gttttaagtg agcccagagc cgatcagaaa cgcgtaatgc aggagttgat
    10561 atcgctctcg acacggctgg tggtcatggt cgatcgcggc cgccggatgt tgcaggagat
    10621 ctaccacgcg ccgccgtcca agatcgacgt catcccgcat ggtattcccg atgtgggctt
    10681 cgtcgatccg acttttttca aagacctgtt cggtgtcgaa ggcaagatcg tgctgctcac
    10741 gttcggactg ctttcgccga acaaagggat cgaatatgtg ctcaacgcca tgccgcgcat
    10801 cctggcggag tttcccgaaa cggtctatat tgtgctgggc gctacgcacc ccaacgaact
    10861 ccgcgagcat ggcgaggctt accgttatag cctggaagtt cttgccaaaa aaaataatat
    10921 cgagaaaaac gtaattttct acaacagttt tgtagagctg gaaaacctga aggaatttat
    10981 tggcgcggcg gatttttaca ttacgccgta cattaatgaa gctcagatta cgtcgggcac
    11041 tttatcctat gcgttcggct gcggcaaagc ggtcgtctcg acgccgtact ggcatgccca
    11101 ggaactgctg gcagacgacc gcggtgtgct ggtgccgttc cgcgattccg acgcgattgc
    11161 gcgggagctt ctcggtttac tgcgcgacga cacacgccgg cacgctatgc gcaagaacgc
    11221 ttaccggttg gggcgtgaaa tggtttggag caacgtagcg cacacgtata tgcattcgtt
    11281 cgagctctca cgcacggagg gtgcggcgca tcctcagaaa tcgctggtta ccaagacact
    11341 ggaccgcaag gcgagggaac tgcccgaggt gaacttgagc cacctctccc ggctgaccga
    11401 ttccaccggg atcttccagc acgccgtttt cagcgttccg aatttttccg aaggctattg
    11461 taccgacgac aatgcgcggg ccttcatttt cacggtgttg ctcagcgaat tgggagtgga
    11521 tccggagaaa gtgcggacgc tatctacaac gtacgcggcg tttcttcggc acgcgttcga
    11581 tccaagtacc aatcgcttcc acaaccacat gagtttcgac cgccgctggc tcgacgagcg
    11641 gggctccgag gattgccacg cgcgcgcact ttgggcgctg ggagtggggg tggggcgctc
    11701 gccctaccgg agcttccagg tgatggccgg acaactgttt gagcaggcgc ttccagcgct
    11761 gctggatttc cggtcgccgc gcgcctgggc gttcgggatt ctcggcattc atgaatacct
    11821 ccgcctgctg agcggggaca gcctggccgc ccaggcgcgc gaaacgctga cttcgcgcct
    11881 gatggatctt ttcgacagga ccgcccagcc ggactggcgc tggttcgagg aacggctcac
    11941 ctacgacaac gccaagctcc cgcacgcatt gattctcagc ggaagggcta cgaaacagga
    12001 agcggtgttg gagcgggggc tggaggcctt gcgctggctg agcgagttac aggtctccga
    12061 gaggggctat tttcaatgta tcggcagcaa cggtttttat agccgcggcg gcatccgcgc
    12121 cagctttgac cagcagccga tcgaagctca cgcaatggtc tcggcgtgtc tcgatgcgtt
    12181 tcgtgttaca gcggattcct ggtggtacaa ccaggcgcag cgcgccttcg attggttcat
    12241 gggcggcaac gatctcgggc tggaattgta ctcctcgaaa accggcggct gccgcgacgg
    12301 gcttcacgtc gaccgcgtca accggaatca aggagccgaa tcaacccttg cttttctgct
    12361 ttccctggcg gaaatgcgcc tggcgcagaa catgctgacc agtttcaaag aaccattcgc
    12421 tttggaatcg tgaccttgcg tcccgtaccg ttaaacggat catcagcgct catggccaat
    12481 aactcacttc acgtcaaacg cacgtcgatc attctcaggc ctgatccgtc acgcgtcctg
    12541 ctgcggccgt tcagccctgg cgacgctcag cggaggggag agattatctc ccgtatcctg
    12601 tcgattccgg aagaggaggt ctgtcctctg ctggacgaag tctccgttga attctcgtcc
    12661 cggcactcgg agatccggca actgttcctg gcgcgtttcg aacaaatccg cgagcaattg
    12721 tggacggacg aagagctgcc ggaagagcgc aaactgttga tcggctccta tttcatggcc
    12781 gaatactctt tggaatccgc ggcgcttttc aacccctcga tagttcctca ccccgaccag
    12841 gcggacctcc cggatggtgc gctgcgattt attatcagcc tgcgcgcggt cggagagggc
    12901 catatttcct cgctcacctt ccggaccggc attctttacg cggacaacca tatcgaagtc
    12961 ccgcctgcgg ccgggttgct gaccgaaccg cgtcaaatcc ccaacgctct ctatgagaag
    13021 cgactgttcg tgaagaagct ggcggaggtc gggttgacct gcgatttcac gcgtcgcgtg
    13081 atgaagcggt tgaaggagca gttcacgctc gacgagcttc gcgaagccct tcagggcgaa
    13141 cagttccgcc tgcccgacgg aatgagccag gaggatcaga gtgcgagcca ggggatctgg
    13201 atgctggctc gctcgaactt cgaggtgcag ttccaaccgg agcagcggct atcggagcgc
    13261 attctgttcc cggccacccc tgcgcagcga aacggcattg aagacgcgcg cttcgtccgc
    13321 gtggaagaag acgatggaac tcagacctat tacgccacct tcaccgcgtt cgatggcagg
    13381 gtgattatgc cgcaactggt ggaaacttcc gacttcctcc gcttccgctt tatcaccttg
    13441 aacggaccgg ctgcacagaa caaaggcctg gcactcttcc cgcggaagat caatggtctt
    13501 tacgcgatgc tctcgcgcca ggacaacgag aacatcttcc tgatgttgtc cgacaatgtt
    13561 cacttctggt atgagcgcac gttgttgatg gagccgaagt ttccgtggga gctgatccag
    13621 atcggcaact gcggatcccc gattgagacg gaggcgggct ggttggtcct cagccatgga
    13681 gtcggaccgt tgcggagata ctgcatcggg gcctttctgc tggaccgcga cgatccaacc
    13741 caagtgatcg ggcggctccg tgaacctcta ctggagccgg tcgagaacga acgtgagggc
    13801 tacgtgccca acgttgtcta tacctgcggc gctcttcttc ataatggcga gctgattatt
    13861 ccctacggcc tggccgatta cgccacgggg tttgcgaccg tcccgcttga cgacgtgctg
    13921 gctgcgatgc attaaggggc tgttgaaaaa aggtgaaaac cgcttgctca cgcgcgcggc
    13981 tcagaaaggt gatcgcatgt ttcgaagcag taaccgagcc gtgaccgtca gggagcggac
    14041 cccgaactcc ctttcatccg gaaaaagagt ggctgtcctg tcgccggtcg cctggcgaac
    14101 tcctccgcgg cagtacggcg cgtgggaaac ggtggcgagc aacatcacgg aagggctggt
    14161 tgcgcgggga tgggatgtga cgctatttgc taccgccgat tcggtaacgc gcgcgcgcct
    14221 tcatgccgtg gtggagaggg gatatgaaga agatcccgcc gtcgatccca aagtggccga
    14281 ataccttcac attccggagg tgttcgagcg ggccgctgac tttgacctta tccacagcca
    14341 ctatgacttc atggcgttga cctatacgcg gctggtccgg acaccggtcc tcaccaccat
    14401 tcacgggttt tcgtcgcctc agatcctgtc catttaccgg aagtacggta atggatattt
    14461 cgtttccatc agcgacgccg atcgcgcgcc gggcctgaat tacctggcga cggtttataa
    14521 cggcatcgat ctttcgctgt atccgtttca gccggccggc ggtgacgatc tgatttttct
    14581 gggccggatt cacccggaca agggggttca cctggcggtc gaggttgccc gccgtgccgg
    14641 tttgccgctg attatcgcgg gcatcatcca ggatcaggcg tatttccgcg agcaggtgga
    14701 accatatctc gatggggaga agatccgcta catcgggccg gtggacgtgc cgggcaagaa
    14761 cgaattgttt gcccgcgccc gggcgctgct gcacctcaac acgatcccgg aacgattcgg
    14821 gctggtcctg gcggaggcca atgcggcggg agtgccggtt attgccatgg accttggctc
    14881 ctgccgcgag gtgatcgaag atgggcagac gggttttctg gtcaatggtg tggaggaggc
    14941 cgtacagtcc ctggagcggc ttcctgaaat cgatcgcggc gcatgccgcc gccgggtcga
    15001 gcagcgtttt tcgatcgaca cgatggtgga gggatacgag cgggtgtacc gcatgatttt
    15061 tgagctggaa gcggcgaagc ggctatgaaa gcagacgttg tcagacgata cggatacaac
    15121 cccattctca ccaaggcgga cattccctat gcggtggaga cggttcacaa cgccgcggtg
    15181 gtgaagcatg ggactgaata catcatgctt ttccggtcac acctgcgcac agggcgatcg
    15241 attatcggcc tggcgcgcag tcccgacgga tttcacttca ctgcggatcc ggagcctttt
    15301 ctggttcccg cgcgggacgg tctttttgcc ggctacgagg aattcggtgt ggaagacccg
    15361 cgcgtgacgc agctcgaaga ggagtacgtc atcacttata gcgcgtactc gcgcaacggt
    15421 gtgcggatcg ccctggcgaa gacgaaggat tttcgccagg tggaacgggt gtccctcatc
    15481 acgcaggcgg actaccggaa cgtggtgatt ttcccggaga aatttgatgg gctgtatgcc
    15541 cggctggacc gcccccattc ggagattgct ccgtggtcga tctggatctc ctattctccc
    15601 gacctgcgtt tttggggtga atctcagctc atcatgaggc cggttcccta tcactgggat
    15661 gagatgaaga tcggcccggg cgcacccccg atcaggacgg acaagggatg gttgtccatc
    15721 taccacgggg tttttgaaac gatggacggg tccgtctacc gcctgggcgt cgctcttcat
    15781 ga
//
LOCUS       DASTMK010000051         6647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_3437, whole genome shotgun sequence.
ACCESSION   DASTMK010000051 DASTMK010000000
VERSION     DASTMK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3437"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3136)
                     /locus_tag="VFQ79_02195"
     CDS             complement(<1..3136)
                     /locus_tag="VFQ79_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138486.1"
                     /translation="MFAHWRPAITLFVLVAASSFPLQAQSTKAELFGTVRDPGNLPVN
                     DAIVELTNTGTETKLSVATDINGNYHFFALPAGTYRISVAKEGFTTLRRDGIVLRVGD
                     QVNLELELRVGDVSQSIEVTAAAPLLQSTRGTASLVVEQKKVISLPLDGRNFIPLIAL
                     SPGVNLPPGNLLPRINGSRPRVSEYIYDGISVLQPEPGQVAFYPIIDAIEEFRVETNS
                     PSAEYGRSNGGVIMVNQKSGSNSLHGTLFEFFRNEALNARNLFATSGAKPRFRRNQYG
                     FVLGGPIQRNKTFFFADWQGTRLDTGVVRTSTVPTSAQKRGVFTQAIFDPLTTSQTPA
                     GYVRQPFPSNTIPDSRIDAATRAVVGRYPAPNVFFGTNEATANNYRRVGNNTTAQDQF
                     DLRLDRYFGPHHRIFGRYSYLRDESTPATPLPDGSGTFTAPFIGRTLTRADSVVGEHS
                     WNLTPTTVNQIRFGFTRRGFNRSSLATGQPATEVSQIPNIPLTSFRDVLPTYDVVGFQ
                     QLGPPANGNAEFTTSVTQFIDNFSWIRGNHSLKAGTDIRLQRLDVLQPPSPTGNFQFT
                     NIFTAGLNAAGTPVANTGNSFASFLAGPVTRFTIDAQSEVLGPRATITEFFLQDDWRA
                     TRRLTVNLGVRYTLNFPSTVADDRGAVFNLQTQKLDYFGQNGFPHSARDLEKANFAPR
                     VGLAYRVTDSFVVRSGYSLTWIEQAGITTPFTTPLFPFIQTLGQQTLDNINPAFYLSQ
                     GPTVTPQLPGPDAGLGQGVFGVQRDNGSGYAQQWNLSLQKTIGENWSFEAGYLGSKLT
                     RLGVPDVNLNQLTVEQLAAGSQLTQQVANPFLGEIPANTSLGTPTIARAQLLRPYPRF
                     TTVTLYRNNVGHSTYHSFQSRMEKRFSHGLTFTVAYTFSRLIDDAGAVFDSAVLTGPV
                     ANFQAADSFNKRLEKDVSTGNVPHILSSGFVYELPFGRGRARSLSGWRDFLAGGWQLA
                     GIVRMQSGSPVAVTQATNLNAFAGFGIQRPNRIADPSLDSDQRTTARYFNTAAFTQAP
                     QFTIGITSRNPVTGP"
     gene            3250..5439
                     /locus_tag="VFQ79_02200"
     CDS             3250..5439
                     /locus_tag="VFQ79_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138487.1"
                     /translation="MFLLSVAAVYPQQGSSIIDWLTALQAIDARLKANDDASAETELR
                     SLHREIAAWANGRADGGVKLPAFPDGQPGHQQLVDYVASLREALEEAERNRPGSVFHA
                     GRIEVNVTANALQVPTASTLEESDYRLRDLPKTADALNLVPGVTIQRIGPRNERGVYV
                     RGFDFRQVPLYMDGIPVYVPYDGYVDLDRFLTYDVSEIQVAKGFTSPLYGPNAIGGAI
                     NMISKAPVKKLNLDLGTGYASGDQVHGFINAGTRFRKFWLQGGFAWLSSDYFPLSGDF
                     QTVPLQPDRDRRNAYQTDNKGRIRTAWTPNDRDQYTFTYAKQTGEKGNPPYAGTDSTV
                     RPRFWQWPQWDKESFYFIGNKSLGETGYVRARFYYDKFDNLLYAYDNANYNSQTLPSS
                     FISPYDDNTYGTTTEFGTKAGSRQTIKASFYFKDDTHREGNLGEPPRTFRDQTYSFGI
                     QDTIQLAARTSAILGFSADHLDVLNAQNLVSGTVQPFPTNNVWAYNPQAGVFHALTES
                     GKVHFTYARKTRLPTIKDRYSYRMGQAIPNPDLREERSDNFEAGYTQLLGLRSFLEVA
                     LFRSNVSNSTQRFFVQPNVFQLRNLGEARYLGGEMGFRTSLTRSLHFSANYTYLSRRN
                     QTMPSVIMLDTPRHKIYSTATYLWRNRLTLLADLLYEGGRWNSNDAGRVLRASSYATA
                     GLGGIARIYRQAELQAGISNLFDRNYFLVQGYPEAGRTFYLNLRYRF"
     gene            complement(5469..>6647)
                     /locus_tag="VFQ79_02205"
     CDS             complement(5469..>6647)
                     /locus_tag="VFQ79_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0138488.1"
                     /translation="EKMEAKLEELPTMAYTFSQPIEFRMLELIEGVGSRSDVVLKIFG
                     DDLDELREQADRAAKVIAQVNGVADLKVQQVSGQPVLSIKTDREAIARYGINVADVQE
                     LIQTAIAGSEAGRVFEGFKRFDLVVRLDPSVRASPSDFKNLLVTTASEQKIPLGQLAK
                     IETEEGPLEVSRENGQRRISIEANVRGRDIGSFVAEARRQIEEQVKLKPGYLMVWGGL
                     WEHLDSGRARLMVAVPFTFLIIFLLLFVTFHSASQAALVFTGIPFAVTGGILSLLIRG
                     MPFSMSAGVGFIAVSGVAVLNGVVMVAFINQNRAGGMSWPEAIRNGTLARLRPVLMTA
                     AVASFGFLPMALSSSAGAEVQRPLATVVIGGLATSTLLTLLVLPALLSVWAGRGRHTT
                     "
BASE COUNT         1398 a         1952 c         1956 g         1341 t
ORIGIN      
        1 ccggcccggt taccggatta cgcgacgtga tgccgatggt gaattgtggg gcctgggtga
       61 aggcggcggt attgaaatag cgcgctgtcg tgcgctgatc ggagtccagg cttggatccg
      121 cgatccggtt cggccgctga attccaaagc ccgcgaaagc gttgaggttc gtagcctgcg
      181 tcaccgctac aggagatccc gactgcatgc gaacaattcc agccagttgc caacctcccg
      241 caagaaaatc ccgccagccg ctcaaggaac gcgcacggcc tcgcccgaac ggcagttcgt
      301 acacaaagcc gctcgaaagg atgtgcggca cgtttccggt ggaaacgtcc ttttccaaac
      361 gcttgttgaa gctatcggcc gcctggaagt ttgcgactgg cccggtgagc accgctgaat
      421 cgaagacggc gccggcatcg tcaatcagcc gcgagaatgt gtaggccacg gtgaaagtca
      481 gtccgtgaga gaagcgcttc tccatgcgcg attgaaacga atgataggtg gagtgcccaa
      541 cgttattgcg atagagcgta accgtcgtaa atcgcggata gggacggagc aattgcgcgc
      601 gggcgatggt gggggttccc aacgaagtgt tggccggaat ctccccgaga aacgggttcg
      661 ccacctgctg ggtcagttgc gagccggcgg cgagttgctc caccgtgagt tggttcaagt
      721 tgacatcggg tacaccgagc cgggtcagct tcgatcccag gtaccctgcc tcgaagctcc
      781 agttttcacc gatcgttttt tgcagactta agttccactg ctgggcgtat ccgctgccat
      841 tgtctcgctg cactccgaag acgccttgtc caagacccgc atcggggccg ggaagctgcg
      901 gcgtcaccgt ggggccttgc gaaagataga acgccgggtt gatgttatcc agcgtctgct
      961 ggccgagagt ttgaataaac gggaaaagag gcgtggtgaa tggagtcgtg attcccgcct
     1021 gttcgatcca ggtgagcgag tagcccgagc gcaccacgaa ggaatccgtg acgcggtaag
     1081 ccaggccaac gcggggcgcg aagtttgctt tctccaggtc ccgcgcgctg tgagggaacc
     1141 cgttctgccc gaaataatcc agcttctgcg tttgcagatt gaagacggcg ccgcggtcgt
     1201 cggcaaccgt cgaaggaaag ttgagcgtgt agcgaacgcc gagattcacc gtcaggcgcc
     1261 gcgttgcccg ccagtcgtcc tgaaggaaga actcggtaat ggtcgcccgg ggaccaagaa
     1321 cttctgattg ggcatcaatc gtgaatcgcg tcactggacc cgccaggaaa gaagcgaaac
     1381 tattgccggt gttcgcaacc ggagtgcccg cggcattcag accggcggtg aaaatgttgg
     1441 tgaactgaaa attgcccgtg gggctgggcg gttgcaagac gtccagtctc tgaagccgaa
     1501 tgtccgttcc ggctttcaga ctgtgatttc ccctgatcca tgaaaagttg tcgatgaact
     1561 gcgtcacgga cgtcgtgaat tccgcgttgc cgttcgcggg agggccaagc tgttggaacc
     1621 cgacgacatc gtaggttggc agcacgtcgc gaaacgaagt aagcggaatg ttcgggatct
     1681 gggaaacctc tgtagccggc tgtcccgtgg cgagcgacga acggttgaag ccgcggcggg
     1741 tgaacccgaa gcggatctgg ttcacggtag tgggcgtcag gttccaacta tgttcgccga
     1801 ccacactgtc ggcgcgcgtc agtgtccggc cgataaaagg cgccgtgaat gttccgctgc
     1861 cgtccggaag aggagtcgcc ggagtggact cgtcgcgtaa atacgagtac cgcccaaaaa
     1921 tcctgtggtg cggcccgaag taacgatcca gccggagatc gaactggtcc tgggccgtcg
     1981 tattgttgcc gacgcgccgg tagttgttgg ccgtcgcttc gttcgtcccg aaaaaaacgt
     2041 tgggggccgg ataccggcct accaccgccc gggtcgcggc atcgattcta gaatctggaa
     2101 ttgtattgga tggaaagggc tgcctgacgt agcccgccgg cgtttgactg gttgttagcg
     2161 ggtcgaagat cgcctgcgta aacacgcccc gcttctgggc gctggtgggg accgtgctgg
     2221 tccggacaac cccggtatcg agccgcgttc cctgccagtc ggcgaaaaag aatgtcttgt
     2281 tcctctgtat tggaccaccg agaacgaacc catactggtt gcgacggaac cgcggcttgg
     2341 caccgcttgt ggcaaacaga tttcgtgcat tcagcgcttc gttgcggaaa aactcgaaca
     2401 gcgttccgtg caggctgttg gatcccgact tctggttcac catgatgacg ccgccgttgg
     2461 agcggccgta ctcagccgag gggctgttgg tttcgacgcg gaactcttca atcgcgtcga
     2521 tgatcgggta gaaagccacc tggcccggct ccggctgcaa gacgctgatg ccgtcgtaaa
     2581 tgtactcgct cactcgcggc cggctcccgt tgatgcgtgg cagcagattg cccggcggca
     2641 gattgacgcc aggcgacaag gcgatcagcg gaatgaaatt gcgcccgtcc agtggaagcg
     2701 aaatcacctt cttctgttcg acgacaagac tggccgttcc acgggttgat tgcagaagcg
     2761 gcgccgcggc ggtcacctcg atggattgag aaacgtcgcc taccctgagt tcgagttcca
     2821 ggttgacctg atcgccgacc cgcaggacga tgccgtcgcg tcgtagcgtg gtgaagcctt
     2881 ccttggcgac ggagattcga taggtccccg ccggcagtgc gaagaagtgg tagttgccgt
     2941 tgatatccgt agctacggag agtttcgtct ccgttccggt gttcgtcaac tccacaatcg
     3001 catcatttac cggaaggttg cccgggtctc gaaccgtgcc gaaaagctcg gctttggtcg
     3061 attgcgcctg gaggggaaaa gaggaggcgg cgacaaggac gaacagagtg attgctgggc
     3121 gccagtgagc aaacataagt cgttataaaa atactacgct atgttagcat gacatttttt
     3181 tcgtgtagta ttgttaacac gacactatct cttttgatca ccatgaagct tactgccctc
     3241 tcactttgca tgttcctttt gtccgttgcg gcggtctatc cgcaacaggg gagttccatc
     3301 atcgactggt tgactgcgtt gcaggccatc gacgcccgtc tcaaggcgaa tgacgatgcg
     3361 agcgcggaaa ccgagctgcg ctctctgcat cgcgagattg ccgcctgggc aaatggacgg
     3421 gcagacggag gcgtgaaact tccggctttc ccggatggac agccgggaca tcagcaactc
     3481 gtcgattacg tggcaagtct ccgcgaggcg ctggaagagg cggagcgcaa ccgtcccgga
     3541 agcgtgttcc atgctggccg gatcgaggtg aacgttaccg ccaacgcact gcaagttccc
     3601 acggcctcga ccttggagga gtcggactac cgcttgcgcg atcttccgaa aacagccgat
     3661 gcgctcaatc tcgtgccggg tgtaaccatt cagcgcatcg gtccccggaa tgaacgcggt
     3721 gtttatgttc gcgggttcga cttccgccag gtgccgttgt acatggacgg aattcccgtc
     3781 tacgtaccgt acgacggata cgtcgatctc gaccgattcc tcacctatga cgtcagcgag
     3841 atccaggtcg caaagggatt tacgtcgccg ctgtatggcc ccaatgccat aggcggtgcg
     3901 atcaatatga tttcgaaggc accggtaaag aagctcaatc tcgacttggg cacaggttac
     3961 gcctccgggg accaggtaca cggattcatc aatgccggca cgcgatttcg caagttctgg
     4021 ctccagggag gattcgcctg gcttagcagc gactactttc cgctgtccgg cgatttccag
     4081 actgtgcctt tacagccgga tcgggatcgc agaaacgcgt atcaaacgga caacaagggg
     4141 cgtatccgca ccgcgtggac tccgaacgac cgggaccagt acacgttcac ttacgccaaa
     4201 cagactggcg aaaagggaaa tcctccctac gcggggaccg attccaccgt acggccacga
     4261 ttctggcaat ggccacagtg ggacaaggag agtttttact tcatcggcaa caagtctctc
     4321 ggagaaacgg ggtacgttcg ggcgcggttc tactacgaca agttcgacaa cctgctctac
     4381 gcgtacgaca acgcgaacta caatagccag acgctgcctt cgtcgttcat cagtccttat
     4441 gatgacaaca cgtacggaac gacgaccgag ttcggaacca aagccggaag ccggcagacc
     4501 atcaaggctt ccttctattt caaagacgac acccatcgcg agggcaatct tggcgaaccg
     4561 ccgcgcacgt tccgcgatca gacctattcc ttcggcattc aggacacgat tcagctcgcg
     4621 gcccggactt cggcgatcct cggattcagc gccgaccatc tcgacgtgct gaacgcgcag
     4681 aacctcgtct ccggcaccgt gcagccattc ccgacgaaca acgtttgggc ctacaatccc
     4741 caggccggag tttttcacgc gctcaccgag tcagggaagg tgcacttcac ctacgctcgc
     4801 aagactcgcc tacctacaat caaggaccgc tactcgtacc gcatgggcca ggcgatcccc
     4861 aatcccgatc tccgcgaaga acggtccgac aatttcgagg ccggctacac gcagttgctc
     4921 ggcctgcggt cgttcctgga ggtggcgctg ttccgaagca acgtttccaa ttcgacccag
     4981 cgattcttcg tacagccgaa tgtgttccag ttgcgcaatc ttggcgaagc gcgatatttg
     5041 ggcggcgaga tgggtttccg gacaagcctc acacggtcgc tccatttctc ggcgaactac
     5101 acctatctga gccgccggaa ccagacgatg ccttccgtga tcatgctcga tacgccgcgc
     5161 cacaagatct actcgaccgc aacttacctg tggcggaacc gtcttaccct gctggcggac
     5221 cttttatatg aaggtggccg gtggaactcg aacgacgcgg gccgggtgct gcgcgccagc
     5281 agttacgcca ctgccggcct cggcggaatc gcacgtatat accgtcaagc ggaactgcaa
     5341 gcgggcatta gcaacctctt cgaccggaat tatttcctgg tccagggcta cccggaggcg
     5401 ggacgaactt tttacttgaa cctccggtat cgcttttgac agcgggcgcg cggccgttgg
     5461 tgggttcgtc aagttgtatg ccggccgcgt cctgcccaga ccgagagaag cgcaggaaga
     5521 accagaagcg ttaacaacgt ggatgtcgcc agtcctccga taacaactgt cgccagcggc
     5581 ctttgaactt ccgcacccgc actggacgag agcgccatgg gaagaaaccc gaagctcgct
     5641 acagccgcgg tcatcaacac cggccgaagg cgcgccaggg tgccgtttcg aatcgcctcc
     5701 ggccaggaca taccacctgc ccggttctgg ttgatgaagg caaccatcac gacaccgttc
     5761 aggaccgcga cgccggagac cgcaatgaag cccactccgg cgctcatcga aaacggcatg
     5821 ccgcgaatca acagcgagag gatgccgcct gttactgcga acggaattcc ggtaaagacc
     5881 aacgctgcct ggctggcgga gtggaacgtc acaaagagca gcagaaagat gatcaggaac
     5941 gtaaagggca ccgctaccat cagccgggca cgacccgaat ccaagtgctc ccaaagacct
     6001 ccccacacca tcaggtaacc gggcttcagc ttgacctgct cttcgatctg ccgccgggct
     6061 tcggcgacaa agctgccgat gtccctgccg cggacgttcg cctcgatcga aatgcgccgc
     6121 tgtccattct cgcggctcac ttccaacggt ccttcttccg tttctatttt tgccaactgg
     6181 cccagcggaa tcttctgctc cgaggccgta gtcacgagaa ggttcttgaa atccgaaggt
     6241 gacgctcgca cagaaggatc caggcgtacg accaggtcaa agcgcttaaa gccttcaaaa
     6301 acgcggccgg cttccgaacc tgcgatggcg gtttgaatca actcctgtac gtccgctacg
     6361 ttgattccgt accgggcgat ggcctcgcgg tccgttttta tactcaggac aggctggccg
     6421 ctgacctgct gcacctttaa gtcggcaacg ccattcacct gcgcgatgac ctttgccgcc
     6481 ctgtccgcct gttctcgcag ttcgtcgaga tcgtcaccaa aaatcttcag gacgacgtcc
     6541 gatcgtgatc cgacgccttc gatcagctct agcatgcgaa actcgatcgg ttgggagaac
     6601 gtataagcca tggtaggcag ttcctcgagt ttcgcctcca tcttctc
//
LOCUS       DASTMK010000052         7151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_259176, whole genome shotgun sequence.
ACCESSION   DASTMK010000052 DASTMK010000000
VERSION     DASTMK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259176"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFQ79_02210"
     CDS             <1..242
                     /locus_tag="VFQ79_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138489.1"
                     /translation="LFNLLAEFQRAAGLFSYQQPASLRAQVLTCGAILGRAGRKLVLH
                     LSRSWGGLAQRIPLLDSILNWQIPTSPKFEPKLVT"
     gene            522..866
                     /locus_tag="VFQ79_02215"
     CDS             522..866
                     /locus_tag="VFQ79_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HEU0138490.1"
                     /translation="MRQLFAFGVAVIMVVSLGSLIAKEGTEKLVYKSKMGAVTFDHWK
                     HQEREAKTGDCKTCHPKLFPEKKTDNLNYKAGMHRPAETKQISCGFCHRAGGKSFESK
                     GNCNKCHVKGKS"
     gene            988..2562
                     /locus_tag="VFQ79_02220"
     CDS             988..2562
                     /locus_tag="VFQ79_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU0138491.1"
                     /translation="MLDTVIVAVYFLAVFAVGFYHSRRERTSRDYFLAGQHVGWFAIG
                     ASLFATNIGSEHFIGLAGSGASTGLAVGAYEWSAGFCLLLLGWLFVPYYLRSRVFTMP
                     EFLERRFSANCRWYLTIVSLFAYVFTKISVSLFAGGILIREVVGWDYMTSAILLVVAT
                     GIYTVAGGLAAVIYTDLFQAFVLIGGAIVLTLVGLNEVGGFEGLRAALPPDYFHMIKP
                     IDHPVYPWLGTTFGTLILGIWYWCTDQVIVQKTLSARNIDHARKGAFFCAALKILPVF
                     ILILPGLIAKALWPGDVTGDNAYPLMVLRLLPPGLTGLMVAALLAALMSSLSSVFNSC
                     STLITMDVYAKLRPGASERRLVVVGRLTTVAIVVISIAWIPFIRYLNAEIYQYLQSVQ
                     AYVGAPITATFLTGILWKGATARAAFTTLVFGGLIGAARFLLDILRNGFGMDLGPLNS
                     VVAFSFLNFSVIVFAVCVLLMMIVSKATTAPVPESLVNLTFDWSQRPQFAAQRGAILW
                     TFLIGGAILGLWIHFA"
     gene            complement(2550..4523)
                     /locus_tag="VFQ79_02225"
     CDS             complement(2550..4523)
                     /locus_tag="VFQ79_02225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0138492.1"
                     /translation="MRAAVGLALILSFGLLGQVPSIDQSLEIKTVSNPVIAPAGRFVA
                     YQVQEPNWKDNIFHTEIWVVMLATGERYQLTKGTKSAMNPKWSPDGKLIAFLSERGEK
                     QQVYVISPTGGEATQLTEFDTGIEDIEWSPDGRHIAFTAPGPDTAERKERKEKYGDFV
                     VVRGDFTMKHLWIIPVALEPKEKAKPQQLTSGEKYTVGDFAWSPDSTQIAFAAARDPD
                     ASSSETSDLYVVDVSSRTVRSLVDTPGPETNPVWSPDGKQIAFETAAGQQYFYYMNRR
                     IGIVSAGGGPPAILSEKFDENPSLIAWGSDGIYFSASQRTTAHLFRIDPATKALQRVS
                     KPDRLVATRFSFTKDFSQIAFAGASQGSAEEIFVSPVKGFQPRLVTNMAEQWRPFNPA
                     TREVIEWKSPDGTIIEGVLLKPADYEPRKKYPLLVAIHGGPTGVDRPILAPDRYYPLE
                     LLAAKGALILKPNYRGSAGYGERFRSLNVRNLGIGDYQDIVSGVDYLIAKGMVDRTRV
                     GAMGWSQGGYIAAFLGAYGDRFKAVSAGAGISNWTTYYVNTDIHPFTRQYLKATPWDD
                     PEIYRKTSPITYINRARTPTLIQHGENDRRVPIPNAYELFQALQDRGVPSKLIVYKGL
                     GHSIDKPREQRAVMQHNYDWFSEWIWGEKPQAK"
     gene            4600..5829
                     /gene="dgoD"
                     /locus_tag="VFQ79_02230"
     CDS             4600..5829
                     /gene="dgoD"
                     /locus_tag="VFQ79_02230"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEU0138493.1"
                     /translation="MKIRGSGGTLTRRALAAGGLGAIAALSAQETRKDGPISPKDKLK
                     VTRLETFLVKPRWLFLKVHTNAGITGLGEPITEGRALTCAEAVKEVEPYLVGKDPRQV
                     THHWQAIYRHAFYRGGPILTSVLSGIDQALWDIKGKALGVPVYELLGGPTRTRVRVYA
                     HARSADQIQQRLKEGFTAFKTGPAKRRPSRYVETPAEVQYAAEKFAEFRRTVGNDIDI
                     GIDFHGAISPATAKLLIKVLEPYQPMFIEEPCQAQNHDVMAEIARGTHLPIATGERVF
                     TKWGFREVLEKGAATILQPDLCHAGGITECRLIAGMAEAYYASIAPHNPLGPISLAAG
                     VQLAASIPNFLCQEQVSLGEGYLKQPFKVREGYLELPTGPGLGIELDENAMADKLSHD
                     WRNPESYDADDGSVADW"
     gene            complement(5793..6827)
                     /locus_tag="VFQ79_02235"
     CDS             complement(5793..6827)
                     /locus_tag="VFQ79_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138494.1"
                     /translation="MTRRKAISTMTAYAALGISRAQQQTPAFRADVRLITVDVLVQER
                     ASGKPLELLGPKDFVLLDDGNRREIVFFEIETTPLDIVFVPYLAGQLNTVKDYNAFIG
                     GWRAAFATFRDQDRAGLVRSLRDADNIALTEDRDRVRDAIRRPSGRAYPPVGYGGRLY
                     DTLCLAATLFPAAAGDRRRRAVIAITDDAEKDSKASIEDLTRAFLTADCTLNIVLLAT
                     AIHRRLTIGRGGPWPIPGRQRDIGGGIPEGASCREAMAATGGLFIPGDEMETALPDLI
                     RKTSLRYLLGFYADPNTGPGFHTLAIRLSEEASRQYPDAEIRHRKGYYVESGTASRLY
                     QSATLPSSAS"
     gene            complement(6824..>7151)
                     /locus_tag="VFQ79_02240"
     CDS             complement(6824..>7151)
                     /locus_tag="VFQ79_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138495.1"
                     /translation="VVLAPKYLAQEVKRKLGLDAVAADDDLRALSNPTGGESRIEEPS
                     GYLLRQALGRIRSRFQVIGVTKGSGPVSLRLTAEGKIKYRDLEFRILSDETSPAAADP
                     GWGERR"
BASE COUNT         1358 a         2121 c         2167 g         1505 t
ORIGIN      
        1 tgctgttcaa tctgcttgcc gagttccagc gcgcagccgg ccttttcagc tatcagcagc
       61 cagcctcact ccgtgcgcag gtactgacct gcggtgccat tctcggccgc gccggtcgca
      121 agctcgtgct tcacctgtcc cgaagctggg gtggtctggc acaacgcatc cctttgctgg
      181 acagcatctt gaactggcaa attccaactt cgccgaagtt cgaaccgaaa ctcgttacct
      241 gagccgccga gcgggcgcga tccgctgctc aaccgctccc gcttcaggtc aaacttcgga
      301 tttcgggctt aggcgagttc cactacctgg aggatctgtc gcggagtaaa gtatggcggt
      361 tcacctatat tgcaacaacg aaccggctga agggcacggc agccggattc gcactacggc
      421 ctctggcgat tgaatagacg gaggcctgcc caaaaccggc gcgatcgcga tagtataaaa
      481 cctgttggca gcatagttcg tctagaaaca gggagattat aatgcgtcaa ttattcgcat
      541 tcggggtggc tgtgattatg gtcgtgagtc tgggcagcct aatcgccaaa gagggtacgg
      601 aaaaactggt ctataagagc aagatgggcg ccgtgacctt cgaccactgg aagcaccagg
      661 agcgggaggc gaagactggt gactgcaaga catgccatcc caagctgttt ccggaaaaga
      721 aaaccgacaa tcttaactat aaagccggaa tgcacaggcc ggccgaaacg aaacagatct
      781 catgcggctt ctgccaccgg gcgggcggca agtcttttga aagtaaaggc aactgcaaca
      841 agtgccacgt caagggcaag tcataggatc cagcccggtt ccggggaccg caggtccgtt
      901 ctaaaagcga tccccggaac attccttcga atcccgccgt caaaatcagc ggcgcaaagt
      961 ctgtgcgtcc cggtaaacta atcgggaatg ctcgacaccg tcatcgttgc cgtctacttc
     1021 ctggcagttt ttgctgtcgg cttttatcat tcgcggcgtg agcgcaccag ccgcgattat
     1081 ttcctcgccg ggcagcacgt ggggtggttc gccatcggcg catccctgtt cgccacgaac
     1141 atcggcagcg aacatttcat cggcctcgcc ggctcaggcg catccaccgg actcgcagtg
     1201 ggcgcttacg agtggtcggc cggtttctgc ctgctgctgc tcggctggct tttcgtgcct
     1261 tactacctcc ggagccgggt ttttaccatg cccgaatttc tggagcgccg gttcagtgcg
     1321 aattgccgat ggtatctgac catcgtttcg ctgtttgcct acgtcttcac gaaaatctcg
     1381 gtgtctttgt tcgcgggcgg catactcatc cgtgaggtgg tgggctggga ttacatgacc
     1441 tccgctatcc tgctggtggt cgccaccggt atctataccg tggctggggg gctggcggcg
     1501 gtcatctaca cggatctgtt tcaggcgttt gtcctcatcg gcggcgctat cgttctcaca
     1561 ctggtgggtc tgaacgaggt gggcggattc gaagggcttc gcgccgcttt gccgccggac
     1621 tactttcaca tgatcaagcc catcgatcat ccggtgtacc catggctcgg cacgacattc
     1681 gggacgctga ttctcggaat ctggtactgg tgcacggacc aggtgatcgt tcagaagacg
     1741 ctgagcgccc ggaacatcga ccacgcccgt aaaggcgcgt tcttctgcgc ggcgctgaaa
     1801 attctgccgg tgttcattct catcctcccc ggattgatcg ctaaagcgct ttggcccggt
     1861 gacgtcaccg gggacaacgc ttatccgctc atggtgctgc gcctgctgcc ccccggattg
     1921 actgggctga tggtcgccgc cctgctcgcc gcgctgatgt cgtcgctgag ctcggtattc
     1981 aattcctgct cgacgctcat caccatggat gtctacgcca aactgcgtcc cggcgcgagc
     2041 gaacgccggt tggtggtggt cggcaggctg acgacggtcg cgattgtcgt catcagcatc
     2101 gcctggattc ccttcatccg gtacttgaat gccgagatct atcaatatct gcaaagcgtc
     2161 caagcctatg tgggagcgcc gatcacagcc acgttcctta cgggaattct ctggaaaggc
     2221 gcgacggcgc gggctgcgtt caccacgttg gttttcggag gcttgattgg cgcggcacgg
     2281 ttccttctgg acattctgcg caatggattc gggatggacc ttggaccctt gaactccgtt
     2341 gtagcgttca gtttcctgaa tttcagcgtg attgtgttcg ccgtctgcgt actactaatg
     2401 atgatcgtca gcaaggccac cacggcaccc gtgccggaaa gccttgtgaa tctcacattc
     2461 gactggtccc aacgcccgca gttcgcggcg cagcggggcg cgatcctttg gacgtttctc
     2521 attggagggg caatcctggg gctctggatt cacttcgcct gaggcttttc tccccagatc
     2581 cactcgctga accagtcata gttgtgctgc atcacggcac gctgctcccg cggcttgtcg
     2641 atgctgtggc cgaggccttt atacacgatc agcttcgagg gcacgccgcg gtcctgaagt
     2701 gcctggaaca attcgtaggc gttgggaatg ggtacgcggc ggtcgttttc gccatgctgg
     2761 atcagcgtgg gtgtccgggc ccggttgatg taggtaatgg gcgaggtctt gcggtaaatc
     2821 tcggggtcgt cccacggcgt ggctttgaga tactggcgcg taaaagggtg gatatcggta
     2881 ttgacgtaat aggtggtcca gtttgaaata ccggctccgg cggaaaccgc cttgaaccgg
     2941 tcgccatagg cgccgagaaa agcggcgatg tagccgccct ggctccaacc catggccccg
     3001 acgcgggtcc ggtcgaccat ccctttggcg atgagataat ccacgcccga aacaatgtcc
     3061 tggtaatccc cgatgccgag attgcggaca ttcagggaac ggaagcgctc gccatatccg
     3121 gcagagccgc ggtaattcgg tttcaggatc agcgctccct tggcggctaa cagttccagc
     3181 ggatagtagc ggtccggcgc caggatcggg cggtctaccc ccgtgggtcc tccatgaatc
     3241 gccaccagca gcgggtattt tttccggggc tcgtaatcgg ccggcttcag cagcacacct
     3301 tcgatgatcg tgccatcggg cgacttccat tcgatgacct cacgcgttgc cggattgaat
     3361 gggcgccact gctccgccat attggtaacg agtcgcggct ggaacccctt cacgggggag
     3421 acgaagattt cttcggccga gccttgggaa gctccggcga aagcgatctg ggagaaatct
     3481 ttcgtgaaag agaagcgcgt ggcaaccagc cggtccggct tcgacacgcg ttgaagcgct
     3541 ttcgtcgccg ggtcgattcg aaacagatgc gcggtggtgc gctggctggc ggagaaatag
     3601 atcccatcgg agccccaggc gatcagagag ggattttcgt cgaacttctc cgacagaatc
     3661 gcgggtgggc cgcccccggc ggaaacgatt ccgatgcgcc ggttcatgta atagaaatac
     3721 tgctggcccg ccgctgtctc aaaagcgatc tgtttgccgt ccggcgacca gacgggattc
     3781 gtttccggac ctggggtgtc gacgagactc ctcacggtgc ggctgctcac atcgacaacg
     3841 tagagatccg aagtctccga agagctggca tcgggatctc tcgccgcggc gaatgcgatt
     3901 tgcgtcgagt ccggcgacca tgcgaaatcg cccaccgtat acttttcacc ggaggttagc
     3961 tgctggggct tggccttctc tttcggttcg agcgcaaccg gaatgatcca caggtgcttc
     4021 atggtgaaat cgccgcgaac cactacgaaa tcgccgtatt tctccttgcg ctccttccgc
     4081 tccgcggtgt cgggtcccgg cgcggtgaat gcaatgtgcc ggccatcggg agaccattcg
     4141 atgtcctcga tgccggtatc gaattctgtg agctgcgtcg cctcgccgcc tgttggagaa
     4201 atcacgtata cttgctgctt ctcaccgcgt tcggaaagaa aagcgatgag cttgccatcc
     4261 ggcgaccact tcgggttcat cgccgatttg gtgcccttgg tcaactggta gcgctcgccg
     4321 gttgccagca tcaccaccca gatttccgtg tgaaaaatat tgtccttcca attcggttcc
     4381 tgcacctggt aggcgacaaa gcggccggcg ggtgcaatta cagggttcga taccgttttt
     4441 atttcgagcg actggtcgat ggatggcacc tggcccagca gcccgaaact cagaatcagc
     4501 gcgaggccca ccgcggcacg catatctatc tattatccgg tgcccggttg cgccgtgggt
     4561 atccgccgcg atagattggc cccttccagg ggagggcaca tgaagattcg cggatccgga
     4621 ggaacactga cgcggcgagc gcttgccgcc gggggtctgg gggcgatcgc cgccctttcc
     4681 gcacaggaaa cccggaaaga cgggcccatt tcgccgaaag acaagttgaa ggtcacgcgg
     4741 ctcgaaacct ttctggtaaa gcctcgctgg ctgttcctga aggtccatac gaacgcgggc
     4801 atcaccgggc tcggcgaacc cattacggag ggtcgcgcgc tgacgtgcgc cgaggcggtg
     4861 aaagaggtgg agccctacct ggtagggaag gacccccggc aggtgacgca tcactggcag
     4921 gccatctacc gccatgcttt ctaccggggc ggccccatcc tcaccagtgt gctcagcgga
     4981 attgaccagg cgctatggga catcaagggc aaagcattgg gcgttccggt gtacgagctg
     5041 ttgggaggcc ccacgcgaac acgtgttcgc gtttacgcgc acgcgcgttc agccgatcaa
     5101 atccagcagc ggctgaagga aggcttcact gcgttcaaga ccggtccggc gaaacggcgg
     5161 ccgtcccgct atgtggaaac cccggccgaa gtgcagtacg ccgctgagaa gttcgcggaa
     5221 ttccgcagga ctgtgggaaa cgatattgac atcggtatcg acttccacgg cgccatcagc
     5281 ccggccaccg ccaagctgct gatcaaggtg ctggagccgt atcagcccat gttcatcgaa
     5341 gagccgtgcc aggcgcagaa ccacgacgtc atggcggaga tcgcgcgtgg cacccatctg
     5401 cccatcgcca cgggcgagcg cgtgtttacg aaatggggct tccgggaagt gctcgagaaa
     5461 ggggccgcga cgatcttaca accagacctt tgtcatgccg gcggcatcac cgaatgccgt
     5521 ctgattgcgg ggatggccga agcctattac gcgtcgatcg cgccgcacaa tccgctcggc
     5581 cccatttccc tcgcggcggg tgtgcaactg gcggcgtcga ttccgaactt tttgtgccag
     5641 gagcaggttt ccctcggcga agggtatctg aagcagccgt tcaaggttcg ggaaggatac
     5701 ctggaacttc ccaccggccc cggcctgggc attgagctgg atgagaatgc catggcggac
     5761 aagctgagcc acgactggcg gaaccccgaa agctacgacg ccgacgacgg cagcgtcgcg
     5821 gactggtaga ggcgggaagc tgtcccggac tcgacgtaat accctttgcg gtgcctaatc
     5881 tccgcatcgg gatattgccg gcttgcttcc tcgctcaaac ggatggcaag cgtgtgaaat
     5941 cccggcccgg tgttgggatc ggcgtagaag cccaacagat agcgcaggga ggtttttcga
     6001 atcaggtccg gaagggcggt ttccatttcg tctccaggga tgaacaggcc accggtagcc
     6061 gccatcgcct cacggcagga ggctccctcc ggaatccctc cgccaatatc gcgctggcgg
     6121 cccggaatcg gccatggccc gcctctgccg atggtaagcc ggcggtgaat cgccgtggcc
     6181 agcagaacga tattcagtgt gcagtccgct gtcaggaatg cgcgtgtcag atcttcgatg
     6241 ctcgctttcg aatctttctc agcgtcgtcg gtgatggcaa tgacggcgcg tctgcggcgg
     6301 tcgcctgcgg ccgcgggaaa cagggtcgcg gccagacaca acgtgtcgta taaccgtccg
     6361 ccgtagccga cgggcggata cgcgcgtccg ctgggccggc gaatggcatc ccggaccctg
     6421 tcgcgatctt cggtcagcgc aatattatcc gcgtcgcgca aagatcgcac caggccagcg
     6481 cgatcctggt cccggaatgt cgcgaaggcc gcccgccagc cgccgatgaa ggcgttgtag
     6541 tcttttacgg tattgagctg tccggccagg tatggcacaa agacaatgtc caacggggtg
     6601 gtctcaatct cgaaaaatac gatctcgcgc cggtttccgt cgtcgagaag aacgaagtct
     6661 tttgggccaa gcaattccag tggcttgccc gatgcgcgct cctggacaag aacatccacc
     6721 gtgatcagcc ggacgtcggc gcgaaaggcg ggcgtctgct gttgagcgcg gctgattccg
     6781 agcgccgcat acgccgtcat cgtgctgatg gctttccgtc gagtcatcgt ctctcacccc
     6841 aacccggatc tgccgccgcg gggctggtct catccgagag tatgcggaat tcaaggtccc
     6901 ggtatttgat ttttccttcg gcggtaaggc gcaggctcac cggaccgctt cccttcgtca
     6961 cgccaatgac ctgaaaacgg cttcgaatac gcccaagtgc ctggcgcaac agatagccgc
     7021 tgggttcttc gattcgggat tctccgccgg tggggttcga gagtgcccgg agatcgtcat
     7081 ccgccgctac ggcatcgagt cccagcttgc gttttacttc ctgcgcgagg tattttggcg
     7141 ccagcacaac t
//
LOCUS       DASTMK010000053         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_611463, whole genome shotgun sequence.
ACCESSION   DASTMK010000053 DASTMK010000000
VERSION     DASTMK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611463"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VFQ79_02245"
     CDS             complement(<1..1271)
                     /locus_tag="VFQ79_02245"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138496.1"
                     /translation="MTITRFLLLFACSWCAWAQKAEVSGFVKDPSGAGVPNAAVQIQN
                     TATDIKAATRTNESGLYVLPLLNPGTYSLTVEARGFDRTVIDNIQIAVGAKVTRNINL
                     NVGTVMEQVTVDGSGINVNTVDASVSAVIDRQFVANIPLSGRSFQSLMTIVPGVAVVP
                     SQGAGTSGEISVNGQRTEANYFTVDGVSANTGVSNTGTIGFSAGYSGSTPGETALGTT
                     QNMISLDALQEFRATTSSYSAEYGRTPGGQFSFTTRSGGNDWHGSLFEYLRNDALNAN
                     NWFGNATRTPKPAERQNDFGGTLGGPLWRNRTFFFVSYEALRLRTPQSGITTHVPSMS
                     LRQSPTGVLQAALNAFPIPNGPDTGNGMATFTTSYSNPASLDSTHPIAVMPAAGDSVP
                     FVVFMARENSSDSAEIPTHAVRVFFDPTNGR"
     gene            complement(1284..2549)
                     /gene="fes"
                     /locus_tag="VFQ79_02250"
     CDS             complement(1284..2549)
                     /gene="fes"
                     /locus_tag="VFQ79_02250"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HEU0138497.1"
                     /translation="MPEPVRQLQSDILERGPEVLARFWRSVEQQGTPLVEQTLSDSRY
                     VSCTFLWRGDAETRSVLVYLPPYTRLSRDAFQMSRVDGTGVWFVSVKLPAKSRLLYQI
                     AVNPPAVMAAMTATPEQNKAFRAAVRPDPFNPRRWPEDAGPATSETFSVIELPGAEPQ
                     LWLTRQAGVPQGKLERRAWKSSLLDNERTVAVYTPPGYASGNPPAGLLVLFDGDDYTT
                     KIPVPVILDNLFAAGRIPPLVAVFIGNAAGARPYELPCNARFAEFVHDELMPWVRTAY
                     NVVNTPQRTIIGGSSYGGLAATCTALKYPETFGNVLSQSGSYWWTPPPDPRKPFAFDL
                     EREPNYIASMFITRPRLPLRFYLEAGIAEISVPGQPRGVLDANRYLRDVLLAKGYEVQ
                     YREFAGGHGALSWRGTFPDALLRTIEMWN"
BASE COUNT          548 a          805 c          891 g          569 t
ORIGIN      
        1 tgccggccat tggttggatc gaaaaatacg cggacggcgt gggtgggtat ttcggcgcta
       61 tcgctcgaat tctcgcgggc catgaagaca acgaacggca cactatcgcc tgcggccggc
      121 atgacggcaa tcgggtgcgt actgtcgagc gacgcgggat tcgagtagct ggtcgtgaag
      181 gttgccatgc cgttgccagt gtccggaccg ttgggaatcg ggaaggcgtt gagagcggcc
      241 tgcaacactc ccgtagggga ttggcgaagc gacatgctgg ggacatgagt ggtgataccg
      301 gactgcggag tgcgcagccg gagcgcttca taggagacga agaaaaaggt ccggttgcgc
      361 cacagcggcc cacccagcgt gccgccgaaa tcgttctggc gctcggcggg tttgggagtg
      421 cgggttgcat tgccgaacca gttgttggca ttcagcgcgt cgttgcgcag gtattcaaac
      481 agcgagccgt gccagtcgtt cccaccagac cgcgtggtaa acgaaaactg gccgcccggt
      541 gtgcgtccat actcggccga gtagctcgat gtcgtagcgc gaaactcctg gagcgcatcg
      601 agtgagatca tgttttgcgt ggtgccgaga gcggtctcgc ccggcgtgga tccggaatag
      661 ccggcagaga agccgatggt tcccgtgttt gaaacgcccg tgttggcgct tactccatcg
      721 acggtgaagt agttggcttc cgtccgctgc ccgttgacgc tgatctctcc gcttgtcccc
      781 gcgccctgtg acgggacaac ggcgacaccg ggaacaatgg tcatcaggga ttgaaagctc
      841 cgtccgctga gtgggatatt cgcgacaaac tggcgatcga tgaccgcgct gacgctggca
      901 tcaacggtat tgacgttgat gccgctgcca tccaccgtta cctgctccat gacggtaccc
      961 acattcaggt tgatgttgcg ggttaccttc gcgccgacgg cgatttggat gttatcgatg
     1021 acggttcggt caaacccccg ggcttccacg gtcaggctat aggtgccggg attgagcaac
     1081 ggcagaacat aaaggcccga ttcgttcgtg cgcgtcgccg ctttgatgtc cgtggcggtg
     1141 ttttgtattt ggacagcggc gttcggaacc ccagcgccgc tggggtcttt tacgaagccg
     1201 gacacctcgg ccttttgcgc ccaggcgcac caggagcagg caaatagtaa taagaatcgc
     1261 gtaatcgtca tatcggtttc tcttcagttc cacatctcga tcgtccggag cagggcatcc
     1321 gggaaagtac ctcgccaact caaggcgcca tgtccaccgg caaactcccg gtactgcact
     1381 tcgtagcctt tcgccagcag cacgtcgcgc aaataccggt tggcatcgag aacgccgcgc
     1441 ggttggccgg gaacggaaat ctccgcgatt ccggcttcga gatagaagcg aagcggaagg
     1501 cgcgggcggg taataaacat cgaggcgata taattcggct cgcgctccag gtcgaaggcg
     1561 aacggcttcc gcggatcggg cggcggtgtc caccagtacg agccggattg cgagaggaca
     1621 ttgccgaagg tttcggggta tttcagggcc gtgcaggttg ccgcgagtcc gccgtagctc
     1681 gagccgccga tgatggtgcg ctgcggggtg tttaccacgt tgtatgcggt gcggacccag
     1741 ggcatcaatt cgtcgtgtac aaactccgcg aagcgcgcat tgcagggaag ctcgtagggg
     1801 cgtgcgccgg ctgcgttccc aatgaagact gctacaagcg gaggaatccg gccggctgcg
     1861 aagagattgt cgaggataac gggaacgggg attttcgtgg tgtaatcgtc gccgtcaaac
     1921 agtaccagca atccggccgg tggattgccg gacgcgtatc ccggcggcgt gtagacggcc
     1981 accgttcgct cgttgtcgag caagctgctt ttccaagcac gcctttccag ctttccttgc
     2041 ggaactcccg cttgccgcgt caaccagagt tgcggctccg cgcccgggag ttctatgaca
     2101 gagaaagttt ccgaagttgc cggacctgcg tcctcgggcc accgccgggg attgaacggg
     2161 tccggccgca ccgcggcgcg aaatgcttta ttctgctccg gagtcgcggt catcgcggcc
     2221 ataaccgcgg gaggattcac ggcaatctga tacagaagcc ggcttttcgc cggcagtttc
     2281 acgctgacga accagacgcc ggtgccatcc accctcgaca tttggaatgc atcgcgtgag
     2341 agccgcgtgt agggaggaag gtaaaccagc accgagcgcg tctcggcatc tccgcgccac
     2401 aaaaacgtgc aggacacgta tcgcgaatcg gatagtgttt gctcgacgag cggagttccc
     2461 tgctgctcca ccgaacgcca gaatctggcg aggacttccg gtcctcgctc caggatgtcg
     2521 ctctggagct gccggacagg ttccggcagg tgacctcgcg gtgcccgcca gcacgttcag
     2581 caacgcaaat gtaatcaaaa agctcttaag tgtgctcatt cgcgcggttc ggtttcggag
     2641 cttaaggcaa ctcaattgca attcctgcgg tacgggtctt tacgttccgc tctgccagtt
     2701 tcaccgaagc ctccgacagg atctccatca ggagccaata ctcggattcg gaaagggata
     2761 gctcctggcc ctgtacctcg attgttacgc gatctatccc gctgcgggag aca
//
LOCUS       DASTMK010000054         5964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_355482, whole genome shotgun sequence.
ACCESSION   DASTMK010000054 DASTMK010000000
VERSION     DASTMK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355482"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2723)
                     /locus_tag="VFQ79_02255"
     CDS             complement(<1..2723)
                     /locus_tag="VFQ79_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138498.1"
                     /translation="MFSARVGFFMVALGLLFPAAIFGQATANIVGTIKDTSEGGVPSA
                     RVTAVNVQTGLRATRESAADGSYSIPLLPAGQYRLEVEKEGFQRYSQSGIMLAVGDNA
                     RLDAILTVGSVAESVTVTAAAPLLETETGTIRGLVDQQRIVDLPLNGRRVTNLMQIQA
                     GVIQRSSGTSEGDAFVVNGSRQSGVYFTLDGGMNTDSYRNYSGVFPNPDAVQEVSIQK
                     SNFSAEYANATGAVVTVATKSGTNEFHGSAFEFLRNGVFNARNFFAARRDSLKRSQFG
                     GTLGGPVIKDKLFFFGSYQGTRTRSDPQLTRQFLPTAAIRQGRFSRPITDPQTQQPFP
                     DNQIPLARLNPATLELLKYIPDPGTPDGQRFVGVPNKDREDEYTAKADANLGAHRLMG
                     RFFRQRYERPFTGRVDDLASMFASDVGRSNQPYQQLTLNDIFTASPQILNSATFALRW
                     RRTHNDWTSVDLPIDFAQAGVKGIAVKDPASVYVNVTGGFLARPGWNYDKRDYDIHIA
                     DTITWIKGSHELKFGGEVLRSKNDIRNDFRTMGLFNFNGNITGDAFADFMLGEVYQFD
                     QGGGEYKELFGTRWGFFAQDTWRALPNLTLTLGLRWDPMFPFHDDLGRTQCFVPGASS
                     VRFPNAPPGYLNDGDPGCPTGGFNSYWQQIAPRFGFAWRATGKTVVRGGFGLFWNPQF
                     TVLYNGFVNAAPFSPQVTRFGVRLEDPYGPAANPFPAAFAPFNPPADAEFFTPLGSFG
                     TFAGDFKPSYMETFNLTLEHEVARNLVARASYIGNLGRRLSYTDDLNYARYAPGATTG
                     NIQQRRPYGDFGAILTTYSAGTSSYHGLQLSMERRVTRGFSFEVNYTFSKSIDETSAD
                     TTPQNPSQTVPMNRTLNRGVSDFDQRHRLIASHVWTLPELSGQNALVRGVLG"
     gene            2825..4189
                     /locus_tag="VFQ79_02260"
     CDS             2825..4189
                     /locus_tag="VFQ79_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138499.1"
                     /translation="MVLALLLLAGLADQVPMRWPSGDPKSLELLSETPVNCILLEQSS
                     WSPELAAEASRREVATMGVIRPDADPIEAAQKALGLKLTGIVLEGDFSDDIAARLRAL
                     AADRKAVLVELPRLAKIRITGQPVIGTFQGVWPGIQIEEDGEAHAAPTGGPWINTNTG
                     FLRYLHAAAPDSTIWIANTPPPGTVFPAERYLQAIGDAAINGARWVVALDADLSKRLI
                     DRESTAVRDWKRIAEHLRFYEEHAGWRELQPAGQLALVQALDRGALVAGGILDMIAVK
                     HTPVRPVPGSVLSDERMKGAKMAVDVDPTVLTDEQKEVLRRFTRSGGTLLNGPPGWQF
                     TPVAENEITIHKDDVAKLDAIWKDVNAMTGRRNLGVRLFNVSSMLSNLLGTSGSSRLV
                     LHLVNYTSYPVENITAHVLGSYKTAVLYSPGHPPKKLAPYPIEEGTGLDIDQMESLAT
                     IVIE"
     gene            4211..5344
                     /locus_tag="VFQ79_02265"
     CDS             4211..5344
                     /locus_tag="VFQ79_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0138500.1"
                     /translation="MTRRELLALLGTAPGIRGTLGAAPENAPTAPVSIARCRSYEEDF
                     VSILSTMFDQVGGLERIVRNKTVTVKLNLTGSPGLRFQGRPLGVTHYSHPKTVGAMAH
                     LMGRAGARRIRFVESCWATAGPLEEYMLDSGWNVRSLANAAPNVEFENTNALGKGKRY
                     ARFKVPGGGYVFPAYDLNYAYDETDVFVSMAKLKDHATCGVTLAMKNIFGITPASIYG
                     DDAGVDEPNESPGKGRVETCHLGKRQPAKSAPQELDRTSSRGPGYRMPRITAELAAAR
                     PIHIAFIDGVETMAGGEGPWIKGIRLVKPGVLFLGTNALTTDVAATAAMGYDPRAQRG
                     TSPFADCDNTLILAEKLGVGTTDLNRIEIRGIPLAEATYRFGA"
     gene            5429..>5964
                     /locus_tag="VFQ79_02270"
     CDS             5429..>5964
                     /locus_tag="VFQ79_02270"
                     /inference="COORDINATES: protein motif:HMM:NF039908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEU0138501.1"
                     /translation="MIDLPEPLIESLPQLTITSIIDILIVAFLIYQFFMIIRGRRSAH
                     ILAGIAVLVLTYLIAIWARLELLRTILAGLAPYTVFALIVMFQSEIRRVLARLGRRRF
                     GLGPSLQRHEAADEIILAVSKLAAQQTGALIVIERDIGLRTFIESGVHLDAALSRDLL
                     LSIFQPGGALHDGAAIVQG"
BASE COUNT         1171 a         1768 c         1867 g         1158 t
ORIGIN      
        1 ccgagcaccc cgcgcaccag tgcgttctgc cccgagagtt ccggcaaagt ccagacgtgt
       61 gaggcgatga gccggtgccg ctggtcgaag tccgaaactc cccgattgag cgtgcggttc
      121 atagggacgg tttgagacgg attttgcgga gtcgtatcgg ccgatgtctc atcgatcgac
      181 ttcgagaagg tgtaatttac ctcgaaggag aaacccctcg tgacgcgccg ctccatcgat
      241 agttgcagcc cgtgataact cgatgtgccg gccgagtagg tggtgagaat ggcgccgaag
      301 tcgccgtaag gacgccgctg ctgaatgttt ccggtggttg cgcccggagc atagcgagcg
      361 taattcaggt cgtccgtata gcttaaccgg cgcccgagat tgcctatgta actcgcccgg
      421 gcgaccaggt tccgcgccac ctcgtgctcg agcgtaaggt tgaaggtctc catgtaactc
      481 ggcttgaagt cgccggcgaa ggttccgaag ctccccaacg gcgtgaagaa ttcggcatcg
      541 gccggcggat tgaatggagc gaaggccgcc gggaacgggt ttgcggcggg gccgtaagga
      601 tcctccagtc tcacaccgaa gcgcgtcacc tgcgggctaa agggagccgc gttcacgaag
      661 ccgttataca ggactgtgaa ctgcgggttc cagaacaacc cgaaaccgcc gcgcacgacg
      721 gtttttcctg tcgcccgcca ggcgaacccg aaccgtggtg caatttgctg ccagtaggag
      781 ttgaatccac ccgtcgggca cccggggtcg ccgtcgttca gatagccggg cggcgcattg
      841 ggaaaacgca ccgaggaggc cccgggcacg aagcactgcg tgcgtccgag gtcgtcatgg
      901 aagggaaaca tcgggtccca gcgcagccct aatgtcaaag tcaggttggg cagggcgcgc
      961 catgtatcct gggcgaaaaa gccccagcgg gtgccgaaga gttccttgta ctcgccgccg
     1021 ccctggtcga actggtagac ctcaccgagc atgaaatcgg cgaacgcatc gcccgtgatg
     1081 ttgccgttga agttgaacag gcccatcgtc cggaagtcgt tccggatatc gtttttcgaa
     1141 cggaggacct caccgccgaa cttgagctca tggctgccct tgatccatgt aatggtatcc
     1201 gcgatatgga tatcgtaatc ccgcttgtcg tagttccagc ccgggcgggc caggaagccg
     1261 ccggtgacat tgacgtagac agatgccgga tctttgactg cgatgccctt cacaccggcc
     1321 tgcgcgaaat cgatgggcaa atcgaccgag gtccagtcgt tatgcgtcct gcgccatcgc
     1381 agagcgaatg tggctgagtt aaggatctgg ggtgacgctg tgaagatgtc gttcagtgtc
     1441 aactgctggt agggttggtt ggagcggccc acgtcggacg caaacatgga ggcgaggtcg
     1501 tcaaccctgc ctgtaaacgg ccgctcgtaa cgttgccgga aaaagcgtcc catcaacctg
     1561 tgcgctccga gattggcgtc ggctttcgcc gtgtactcgt cctcacggtc cttgttgggt
     1621 actccgacga acctctgtcc gtcgggcgtt ccggggtcgg gaatgtactt aagcagttcc
     1681 agggtcgccg ggttcaagcg cgcgagggga atctggttgt ccgggaacgg ctgctgtgtc
     1741 tgcgggtcgg tgatggggcg ggagaacctg ccttgccgga ttgccgccgt gggcagaaac
     1801 tggcgggtca gttgcgggtc gctacgcgtc cgcgtgccct gatacgagcc gaagaaaaag
     1861 agtttatcct tgatgaccgg accgccgagc gttccgccga attgcgaccg cttaagcgaa
     1921 tcgcggcggg cagcgaagaa gttgcgcgcg ttgaagacgc cgttgcgcag gaactcgaag
     1981 gcggagccgt gaaactcgtt ggtcccggac ttggtcgcca cggttaccac agcgccggtc
     2041 gcgttggcgt actccgcgct gaagttactc ttctggatac tgacttcctg gacggcgtcg
     2101 ggattgggga agacgccgct gtagttgcgg taggagtctg tattcatgcc gccgtcgagc
     2161 gtgaagtaga cgccgctctg cctgctcccg ttgacgacga aggcatcgcc ttcgctcgtg
     2221 ccgctggagc gttggatgac acccgcctgg atctgcatca ggttggtgac ccggcggccg
     2281 ttgaggggca ggtcaactat gcgttgctgg tcgacgaggc cgcgaatcgt cccggtctct
     2341 gtctcgagca gcggtgccgc ggctgtcact gtcacagatt cagccaccga acccacggta
     2401 agaatggcgt ccagcctggc gttgtcgccg acggcgagca tgattccgga ttggctgtaa
     2461 cgctggaacc cttccttctc tacctcaagc cggtactgcc cggcgggcag aaggggaatc
     2521 gaatagctgc catcggccgc ggattcccgg gtcgcacgca gacccgtctg cacattcacg
     2581 gcggtcacgc gcgcgctcgg cacaccccct tcggacgtat ctttgatcgt cccgacaata
     2641 ttcgctgttg cctgcccaaa aatggcagcc ggaaacaata atcccagcgc caccataaaa
     2701 aagcccactc ttgctgaaaa caagctgtca cctcctgcgg attaaatgtc aattgttaat
     2761 atgctatgca gccgttggag aaacagcaag catcagatcc ctatggacat cgtaaattaa
     2821 gagtatggtt ctagcgttgt tgcttctggc gggactggcg gatcaggtgc ccatgcgctg
     2881 gccttcgggc gatccgaagt cgctggaatt gctgtccgaa actccggtca attgtattct
     2941 gctggagcag agttcgtggt ccccggaact ggcggcagag gcgtcgcggc gcgaagtggc
     3001 caccatgggc gttatccggc ccgatgcgga ccccattgaa gccgcgcaaa aagcgctggg
     3061 actcaagctg accggcatcg tgctggaagg cgatttcagc gacgatatcg ccgcacggct
     3121 gcgcgcgctg gcggcggatc gcaaagccgt gttggtggaa ttgccgcggc tggcgaaaat
     3181 ccgcattacc ggacagccgg tgatcggcac ttttcagggg gtctggccgg gcattcagat
     3241 cgaggaagac ggcgaggcgc acgctgcgcc caccggcggt ccatggatca acaccaatac
     3301 aggctttctg cgctaccttc acgcggcagc cccggattca acgatctgga tcgccaacac
     3361 gcctcccccc ggcaccgtct ttccggcaga gcgatacttg caggccatag gcgatgccgc
     3421 catcaatggc gcgcgttggg tggttgccct ggatgcagac ctcagcaagc gcctgataga
     3481 ccgcgagtcc acggcggtgc gcgactggaa acgcattgcc gaacatctcc gtttttatga
     3541 agagcatgcc gggtggcgcg aattgcaacc ggccgggcag cttgccctcg tacaggcact
     3601 ggacaggggc gcccttgtgg ccggcggcat cctcgatatg atcgcggtca aacatacccc
     3661 ggtgcgcccg gtgcccggca gcgttttatc cgacgagcgg atgaagggcg cgaagatggc
     3721 cgtcgacgtg gatcccaccg tcctcaccga cgaacagaag gaagtattgc ggcggttcac
     3781 ccgatccggc ggtacgctgc tcaacggccc gccgggatgg cagttcactc cggtagcgga
     3841 aaatgagatc accatccaca aggacgacgt ggcgaaactc gatgccatct ggaaagacgt
     3901 gaacgcgatg accggacgcc gcaacctcgg tgtgcgcctc ttcaatgttt ccagtatgtt
     3961 gtcgaacctg ctcggcacct ccggcagcag caggctggtt ctgcacctgg tgaactatac
     4021 cagctatccc gtcgaaaaca ttaccgcgca cgttctcggg tcgtacaaaa ccgcagtgct
     4081 gtattccccg ggccaccctc ccaagaaact ggcgccctat cccatcgaag aagggaccgg
     4141 cctggacatc gatcaaatgg agagcctggc caccatcgtt atcgaatagg cttcccttta
     4201 tactggaggc atgaccagac gcgaactgct ggccctgtta gggacggctc ctgggattcg
     4261 cggcacactc ggagcagcgc cggaaaatgc tcccaccgcc ccggtttcca ttgcacgatg
     4321 ccgttcctac gaggaagatt tcgtatcgat cttatctacg atgttcgacc aggtcggcgg
     4381 gctggagcgc atcgtccgga acaagacggt aacggtgaaa ttgaacctga cgggcagccc
     4441 cggtctgcgc tttcaggggc gccctctcgg cgtcacccac tattcgcatc cgaaaacggt
     4501 gggtgcgatg gcgcatctga tgggacgcgc gggcgcgcgg cgcatacggt tcgtcgagag
     4561 ctgctgggcc accgccgggc cgctcgaaga gtacatgctc gattcgggct ggaacgtgcg
     4621 ctccctcgcc aatgccgccc cgaatgtgga attcgaaaac acgaacgcgc tgggcaaagg
     4681 gaagcgttat gcacggttca aagtgccggg cggcggatat gtgtttccgg cgtacgatct
     4741 gaactacgcc tatgatgaaa cagacgtttt cgtctccatg gccaagctga aagatcacgc
     4801 cacgtgcggt gtgaccctgg cgatgaagaa catcttcggt attacgccgg cttccattta
     4861 cggcgacgat gccggtgtgg acgagccgaa cgaatcgccg gggaagggac gggtggaaac
     4921 gtgccatctc gggaagcggc agccggcgaa aagcgctccc caggagctcg atcgcacatc
     4981 gagccgcgga cccggctacc gcatgccccg catcaccgcg gaacttgccg cggcccggcc
     5041 catccacatc gcgttcatcg acggggttga aacgatggcc gggggcgaag gcccgtggat
     5101 caaaggcatc cggctggtga agcccggcgt gctgtttctc ggaacgaatg cgctcacaac
     5161 ggatgtggcg gcaacggcgg caatgggtta cgatccgcgt gcccagcggg gcacatctcc
     5221 gttcgccgat tgcgacaaca cgctgattct tgcagagaaa ttaggtgtgg gcaccactga
     5281 tttgaataga atagagatca gaggcatccc tctagcggaa gctacatacc gttttggcgc
     5341 ctgaggagcg ggacgcttaa agtgagcccg cgggtgtcag cagaattctt ccggtccaac
     5401 acccggacag gcgtataccg ccacagcgat gatcgatttg ccggagccat tgatcgagtc
     5461 cttaccgcaa ctgacgatta cgtccatcat cgacatcctg atcgtcgcct tcctgattta
     5521 ccagtttttc atgattattc gcggacgccg gtccgcgcat atactggccg gaattgcggt
     5581 tctcgtcctc acctacctca tcgccatctg ggcgcggttg gaactgctgc gcaccatcct
     5641 ggcgggcctg gcgccttaca ccgtatttgc cctcatcgtg atgttccaat cggaaatccg
     5701 gcgcgtgctg gcgcgccttg ggcgccggcg attcgggttg gggcccagct tacagcgcca
     5761 tgaagccgcg gacgagatca tcctggcggt ctcgaagctg gcggcgcaac agaccggcgc
     5821 gctcattgtc atcgagcggg acatcgggct tcgaactttc atcgaaagcg gcgtacatct
     5881 ggatgcggcg ctgtcgcgcg atctccttct gtccatcttc cagccgggcg gagcgctgca
     5941 tgacggcgcc gccattgtcc aggg
//
LOCUS       DASTMK010000055        10494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_291402, whole genome shotgun sequence.
ACCESSION   DASTMK010000055 DASTMK010000000
VERSION     DASTMK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291402"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1874
                     /locus_tag="VFQ79_02275"
     CDS             <1..1874
                     /locus_tag="VFQ79_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138502.1"
                     /translation="SGRRHNDVRAGNGGDSRNVFYRLFGLPRETFNDLLGGRQQDTGF
                     DQHGLHTKFAARPRPDQLVSLWYQRGVQDGVHSYKDLLGGLGRLQSTFEPQVLNFFYA
                     RYEKLSLGALDSLSGTFSINSQTDGGLRQNLRLTDAITRDYNRVDAYGYSSQATTHFG
                     NRVFVAFGGDIYDERIRSDRDITNPVRATVENVRPLYPDGSTYRTYGLFGQSNVEVLP
                     SRLRIGFGGRLTGIQFRTQEESRFGVPESSQWFRDVTFNTSVSWQLTGNFGLQGLVSR
                     GFRAPNLNDLGAIGLNDLGYEIPAAEAIPAGALLSNDAGEGALPKGGSIEELRPESLM
                     NYEFGVRFTARRLYTRVQLFDAELYDPIVRRTLLFPANGIPTQLAGNPVTLLPQTAAQ
                     RELGVATVATGFDPRAVKAFVNDGRARYYGFEWLANYALSGRWSLDAGYSYILGRELN
                     PNRFARRLPPQSGSLAVRYVPSGRRFWMEASFAASGAQERLSGGDRDDERIGASRRRQ
                     DIADFFRGARVASYLDATGQVFLPTGETLQQIQNRVLPLGATINGVRVADDNTRVPVY
                     LSTAGWATLGLRAGVPFRERWQVVAGLQNLTDKSYRVHGSGLDSPGINGYVSLRYTF"
     gene            1956..3203
                     /locus_tag="VFQ79_02280"
     CDS             1956..3203
                     /locus_tag="VFQ79_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU0138503.1"
                     /translation="MSEVIDNRARRIRILKDIIKHLHAGHSPELVREQLRQMVRETDH
                     AEMVAMEQELMAEGMPVEEIQSMCDLHSQVTRDVLVQLPASPLPPGHPVDTFRRENAA
                     LTETVARMRSAMAKIGALPEDAAPDGLLFEWRQSFNDLMDIDKHYQRKEHTLFSRLER
                     HGITGPSKVMWAKDDEVRGLLKRLGTELQRQNPSAGEAKAIAMKFAAPALSAVEEMIY
                     KEEHILLPMSLNTLTQEDWAEIWASSPQYGWCLVEPRQGYRPPSAVLPDALELSSKEA
                     IQFPTGNLSLEQLTLIFSTLPVDLTFVDADDRVRFFSEGPNRVFARSKAIIGREVQHC
                     HPPKSVDVVDRILSDFREGRQNVAEFWIEFHGRFVHVRYFAVRDADGKYAGTLEVTQD
                     VTRIRQLQGERRLLEYDIAPAVA"
     gene            complement(3269..4075)
                     /gene="lpxA"
                     /locus_tag="VFQ79_02285"
     CDS             complement(3269..4075)
                     /gene="lpxA"
                     /locus_tag="VFQ79_02285"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEU0138504.1"
                     /translation="MPVDSSAAVSPTARIHPEACIGPQCVIGDYCVIESDVTIGRGCR
                     LEPYVYVKRWTTMGEENEISAGTVLGTDPLDKVFTGERSYLRIGSRNQIREHYTISRG
                     TKPESSTDIGDDNYIMTSGHIAHNSKVGNHTVIASCALVAGYVEIEDQAFISGGVVIH
                     QFSKIGRLAMIGGNTRVNLDVPPFFLYSDFNVTPKGLNLVGLRRAGFRASDISPLKQA
                     YRLLFDSGLKLQDALTRIEAECPTEHTLHLVRFVRSSERGICRRAPVVAG"
     gene            4128..5150
                     /gene="lpxD"
                     /locus_tag="VFQ79_02290"
     CDS             4128..5150
                     /gene="lpxD"
                     /locus_tag="VFQ79_02290"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU0138505.1"
                     /translation="MRVRELAEWLVSVFEGDGDKEITDVAAVEDAGPGDLAFVANRKA
                     AQAAFESETGCLIVPLDFPESDRTVIRSADPRAAFARAVRRFRPTRPVQPGIHPTAMI
                     AEDATLGEDVAVGPHTSIGSGASIGAGSVIGAGCAIGRDVKLGVSCVLYANVTIYDDV
                     EIGDRAILHSGSVIGADGFGFARVGARYEKFPQVGTVRIGDDVEIGANSCVDRAALGV
                     TSIGNGTKLDNMVHVAHNCRIGSNVVVAAQTGFSGGVVVEDNVIVGGQVGIGDKARIE
                     SGAVLGSGCGILTSKIVRGGQVVWGTPARPLREHLEQLASLSRLPEMRREFAELRTRL
                     EALEGK"
     gene            5150..5761
                     /locus_tag="VFQ79_02295"
     CDS             5150..5761
                     /locus_tag="VFQ79_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138506.1"
                     /translation="MLVVVGGHSRNIGKTSVAAGIIAALPEAHWSAVKITQHGHGICS
                     PAGTPCDCAIEYSHPFALDEEKRPDGTDSGRFLAAGAKRAYWLRTPSGQLGHAIPDLR
                     KIIESSPNTIIESNSILRFLRPDLYIVVVDYTIADRKESSWLYMDRADAFVEIQRGTG
                     PPPSPWPDVPHKWLDGKPRFRVAPPDYVPRELADFVRQRLTAS"
     gene            complement(5765..6193)
                     /locus_tag="VFQ79_02300"
     CDS             complement(5765..6193)
                     /locus_tag="VFQ79_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138507.1"
                     /translation="MTRRWAFVLLLGSLSGGITAAQSSSEQSKENVQTQEPPEEDVAY
                     APKEYSFNPLQAEKELKVGNFYFKKGSFKAASSRFLEATRWNPGFAEAFRRLGEARDR
                     LKDDEGARQAYKKFIELAPDDKEADSVRKRLEQLARASAK"
     gene            6213..8045
                     /gene="uvrC"
                     /locus_tag="VFQ79_02305"
     CDS             6213..8045
                     /gene="uvrC"
                     /locus_tag="VFQ79_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU0138508.1"
                     /translation="MDLREKAGSLPLLPGVYLYKDGHGTVLYVGKAKSLRHRVRSYFS
                     EDKIGDIKTGSLLSEATDIDYILVDNEKEALALENNLIKQWKPRFNILLRDDKTYPYI
                     KLTNEKFPRVYVTRRLKKDGSSYFGPYFPGNLAHRLVHFINRHFKVPSCSVDLGRRHT
                     HPCLEYHIHRCLGPCVEGLTTAEEYAEAVRSVRLFLEGRHRDLTRELRRRMEAAAEAM
                     RFEEAASLRDLIATVEEMEQKQKMAAAEGEDIDIYAFYAEPPLVAVNLFHLRNGRTVD
                     RREFFWEDQIDFDPAEFFSALLKQVYLDQQYVPGLIHVPVEFEDRELLEEILSEKRGR
                     RVEIHTPQRGRKKAMLGLVETNAKSSFDQRFRVLKPSSKAIQGALEEALNLPAAPKRI
                     ECFDISHIQGTDKVASMVVWEDGRMKKSGYRKFIIRSVIGNDDFASMREVVTRRYTRL
                     KENNQPMPDLVLIDGGLGQLHAAAEALEAIGIINQPLAAIAKREEIIYVFGQEDEPVK
                     LDRFSPVLHLVQSIRDEAHRFAVTFHRTRRNAARLTSELAEIKGVGQATMGKLLRHFG
                     SAKRVRQATEHELHAVVGRTAARRITAYYAQSVTSDETQSEILQ"
     gene            8042..9730
                     /locus_tag="VFQ79_02310"
     CDS             8042..9730
                     /locus_tag="VFQ79_02310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0138509.1"
                     /translation="MTKRRVAAVTLTLMTLLLTNAAEAQREADVILLNGRIWTVNPAQ
                     PEAEAVACGGGRILAVGSNAEIRQWSGAKTKTIDLAGRRVVPGFNDAHVHFYTGGTHL
                     TSVQLRTARSEAEFRERIAAFAAKLEPGKWITGGDWDHESWTPARLPSRQSIDAVTPD
                     HPVFVNRLDGHMGLANSVALKMAGIDRNTPDPAGGSIVRDDAGEPTGVLKDAAMGAVY
                     RLMPPPSREDVLNAIRAAMRYAAENGVTSVQDMSASPEILKAYQELLASDELTVRVYG
                     HQPLPNWQRLANAGIRAAFGGDKLRIGGLKGFSDGSLGSTTALFFEPYLDAPATSGLA
                     ADEMIPPSKMLDNISGADAAGLQIAVHAIGDKANSMVLDLFASVAQKNGARDRRFRIE
                     HAQHLRKQDMGRFGQAGVIASMQPYHLIDDGRWAENRIGPERAKGSYAFRSLLDNGAV
                     LAFGSDWYVAPMEPLMGIYAAATRIPLDGKKRDGWIPEEKITVAEAVRAYTMGSAYAS
                     FEEKQKGSIEPGKLADMAVLSDDILALPPARIREVRVAMTIFDGRVIHTREAGK"
     gene            complement(9758..10318)
                     /locus_tag="VFQ79_02315"
     CDS             complement(9758..10318)
                     /locus_tag="VFQ79_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138510.1"
                     /translation="MSYKRITAAFCTSIVALAAVILTRAEEMKSADPSTPLQVTVSQS
                     ILNPDGTRNLSLQLKNTSANSIAAYALRLDILDAASGKLIAVADRTIIRSTDGPTARL
                     GYAPGEMWSDALQVPIIGDNIQHTLSVDYVKFRTTDKPSAWGPDTRKESIRIEATIAG
                     YRAERARLRQLLKASGTQAVIDDLAQ"
BASE COUNT         2131 a         3104 c         3257 g         2002 t
ORIGIN      
        1 cttccggccg tcgccacaac gatgtccgcg cagggaacgg cggagattcc cgcaacgtct
       61 tctaccggct tttcggcctg ccgcgggaaa ctttcaacga cctgctcggg ggcaggcagc
      121 aggacaccgg cttcgaccag cacggattgc acaccaaatt cgcggcacgg ccgcggccgg
      181 atcaactcgt ctccctgtgg tatcagcgcg gcgtgcagga cggagtgcac agttacaaag
      241 atctgctggg cggcttggga cgactgcaat cgacgttcga accacaggtt ctgaatttct
      301 tttatgcccg gtacgaaaag ctcagcctgg gagcgctgga ttcgttaagc ggaacgtttt
      361 ccatcaactc gcagacggac ggggggctgc gccagaacct tcgcctcacg gatgcgatta
      421 cgcgggatta taaccgggtg gatgcttacg gctactcgtc ccaggcgaca acacatttcg
      481 ggaatcgcgt gtttgtggcg ttcggcggcg atatctacga tgaacgcatc cggtctgacc
      541 gggacatcac gaatccggtt cgcgcgacgg tcgaaaacgt gcgcccgctc tatccggacg
      601 gctccactta ccgcacatac gggttattcg gccagagcaa cgtggaagtt ctgccgtcgc
      661 gtcttcgtat cggcttcggc gggcggctga cgggaatcca gtttcgcacg caagaggaga
      721 gccggttcgg tgtgccggaa tcgtcgcagt ggtttcgcga tgtcacgttc aacaccagtg
      781 tgagctggca gcttacgggc aacttcgggc ttcagggact tgtcagccgc ggcttccgcg
      841 cccccaacct gaacgatctt ggagcgatcg gactgaacga tctcggctac gaaattcccg
      901 cggcagaagc cataccggcc ggcgccctgc tcagtaacga cgcgggcgaa ggtgcgctgc
      961 cgaaaggcgg atctattgaa gagttgcggc cggaatcgct catgaactac gaattcggag
     1021 tccggttcac ggcacgccgg ttgtacacca gagtgcagct attcgacgcc gagctttacg
     1081 atccgattgt ccggcgcacg ctgcttttcc cggcgaacgg gattcccact caactggcag
     1141 gcaacccggt gacactactg ccgcagaccg ccgcgcagcg tgaactcggc gtcgcgaccg
     1201 tcgccacggg tttcgatccg cgcgcggtga aagctttcgt caacgacggc agggcgcgat
     1261 attacgggtt cgaatggctg gccaattatg cgctctcggg ccgctggtct ttggatgccg
     1321 gatacagcta cattctgggg cgcgagctga atccgaaccg ctttgcgcgg cgcttgccgc
     1381 cgcagtcggg ctcactcgcg gtgcggtatg ttccctcggg ccgccggttc tggatggagg
     1441 cctccttcgc ggcatcaggc gcgcaggaac ggcttagcgg aggcgaccgc gacgacgaac
     1501 gcatcggtgc atcgcggcgc cgccaggaca tcgcggactt cttccgcggc gcgcgcgtcg
     1561 catcgtatct cgacgccacc ggccaggttt ttcttccaac cggcgaaacg ctccagcaga
     1621 tccagaatcg cgtgctacca ttgggcgcga ccatcaacgg agtgcgcgtc gccgatgaca
     1681 atacgcgtgt tccggtgtat ctttccaccg ccggatgggc cacgctgggg ctccgcgccg
     1741 gcgtgccgtt ccgtgagcgc tggcaggttg tggccggtct tcagaatctc accgataaaa
     1801 gttaccgggt gcacgggtcg ggcctcgact ccccgggtat caacggctac gtgagcctgc
     1861 gatacacgtt ttaacggccg cgcgtgggtg gtgtgactga agtcataggt tcaggcaaag
     1921 aagacctgtt actctgaacg cgaaaggtga tcagagtgag cgaagtcatc gataaccgag
     1981 cccgccgtat ccggatactg aaagacatca tcaagcatct gcatgccggt cattcaccgg
     2041 agctggtccg ggagcaactc cggcaaatgg tgcgggaaac cgatcatgcc gagatggttg
     2101 ccatggagca ggaactcatg gcggagggga tgccggtcga ggaaatccag tcgatgtgcg
     2161 acctgcattc ccaggtgacc cgcgacgtgc tggtgcagct tccagcctca ccgctgccgc
     2221 cgggccaccc ggtggatacc ttccgccgcg aaaacgcggc tttaacggaa acggtggcgc
     2281 ggatgcggtc ggcgatggcg aaaatcggcg cgctgccgga agatgccgca ccggatggat
     2341 tgttgttcga atggcggcaa tcgttcaatg acctgatgga tatcgacaag cattaccagc
     2401 gcaaggagca taccctgttc tcgcgcctgg agcgtcatgg catcaccggg ccgtcgaagg
     2461 tgatgtgggc aaaggacgat gaagttcggg gactgctgaa gcggctgggc accgagcttc
     2521 agcgccagaa cccgagcgcc ggtgaggcca aagccatcgc gatgaagttc gctgctcccg
     2581 cgttgagcgc cgtggaagag atgatttaca aggaggagca catcctcctg cccatgtcgc
     2641 tcaacaccct tacccaggag gattgggcgg aaatctgggc ctcctctccc cagtacggct
     2701 ggtgcctggt ggagccgcgc cagggttaca ggccgccgtc ggcggttctt cccgatgcac
     2761 tggagttgtc ctcgaaagaa gccatccagt tcccgacggg aaacctgagc ctggaacagc
     2821 ttaccctgat cttctccacg ctccctgtgg atcttacgtt cgtcgatgcc gacgaccggg
     2881 tgcggttttt ctccgaaggt ccgaatcgcg tcttcgcccg gagcaaagcc atcatcgggc
     2941 gcgaagtaca gcactgccac ccgccgaaga gcgtcgacgt agtggatcgc atcctctctg
     3001 attttcgcga ggggcggcag aacgtggccg agttctggat cgagttccac ggccgttttg
     3061 tgcatgtgcg ctacttcgcc gtgcgcgatg cggacggcaa gtacgcggga acgctcgaag
     3121 tgacgcagga tgttacgcgt atccggcaac tccagggcga gcggaggctg ctcgaatacg
     3181 acatcgcacc cgcggtagcg tagttgaccg ctccgtgacc gtcgcggctc agaaacggag
     3241 ccgcgcgcgt caagcggcat cggatagctt aacccgcaac aaccggcgcc cgccggcaga
     3301 tgccgcgttc ggacgatcgc acaaatcgaa ccaggtggag cgtgtgctcc gtcggacatt
     3361 ccgcttcaat tcgcgtcagc gcgtcctgaa gcttcagacc tgaatcaaaa agcagccggt
     3421 acgcctgctt gagcggggag atatcgctcg cgcgaaaacc ggcgcgccgt agacccacca
     3481 ggttcagtcc tttcggcgtg acgttgaaat cggaatacaa gaagaacggc ggcacatcca
     3541 gatttacgcg cgtattgccg ccgatcatcg ccaggcgtcc gattttcgag aactggtgaa
     3601 tcaccacgcc cccggaaatg aacgcctggt cctcgatctc cacgtacccg gctaccagcg
     3661 cgcaactggc gatgacggtg tggttgccga ctttcgaatt gtgggcgatg tggcctgacg
     3721 tcatgatgta gttgtcatcg ccaatatccg tggaagactc gggcttggtg ccgcgcgaga
     3781 tggtgtagtg ctcgcgaatc tggttgcggc tgccgattct caaatagctg cgctcgccgg
     3841 taaacacctt gtcgagcgga tctgtgccga gcacggttcc cgccgagatc tcgttctctt
     3901 cccccatcgt tgtccagcgc ttcacgtaga catacggctc cagccggcac ccgcggccga
     3961 tcgtgacatc cgattcaatg acgcaataat ccccgatgac gcattgcggc ccgatgcagg
     4021 cttccggatg gatgcgcgct gtgggtgaca cagcggcgga ggaatcaaca ggcataacct
     4081 taaatgatag cggcgtgctc gggggcaagt cgcggaagga ggaaaggttg cgagttcggg
     4141 aactggcgga atggctcgtg tcagtgtttg aaggcgacgg tgacaaggaa attacggatg
     4201 tggcggccgt cgaggacgcc ggccctggcg acctggcttt cgtcgccaac cggaaggccg
     4261 cgcaggcggc gttcgaatcg gagaccggtt gtctgatcgt cccactggat tttcccgaat
     4321 cggacaggac agtgatccgg tcggccgacc ctcgtgcagc ctttgcgcgt gctgttcgca
     4381 ggttccggcc tacacggccg gtacagcccg gcatccaccc cacggcgatg atcgcggagg
     4441 atgcaacact gggcgaggac gtagccgtcg gaccgcatac ctccatagga agcggcgcca
     4501 gcatcggagc cggcagtgtt atcggcgcgg gctgcgccat cgggcgagac gtgaagctcg
     4561 gtgtctcgtg tgttctttac gccaacgtga caatctatga cgacgtcgag atcggcgaca
     4621 gggccatttt gcattctggc agcgtcattg gcgcggatgg cttcggattc gccagggttg
     4681 gcgcaagata cgagaagttt ccgcaggtgg gcacggtccg aatcggcgac gatgtcgaaa
     4741 tcggtgcaaa cagttgcgtg gaccgtgcgg cgctcggcgt cacctccatc ggaaacggca
     4801 ccaagctcga caacatggtg cacgtggctc acaattgccg tatcggatcg aacgtggtcg
     4861 tcgccgccca aacggggttt tccggcggtg tggtggtgga agacaacgtc atcgtcggtg
     4921 ggcaggtagg catcggcgac aaagcccgta tcgaatccgg cgcggtgctc ggctccggct
     4981 gcgggattct cacttcgaag atcgtccgcg gaggacaggt ggtttggggt acgcccgcgc
     5041 gtcctttgcg ggaacacctg gagcaactgg cgagcctgtc ccggctgccc gaaatgcgcc
     5101 gcgagtttgc cgaactgcgg acccggctcg aagctctgga aggcaagtaa tgctggtggt
     5161 ggtgggcggt catagcagga acatcgggaa aacctcggtc gccgcaggca tcatcgccgc
     5221 cctgcccgag gcccattgga gtgccgtgaa gatcacgcag cacggccacg gcatctgttc
     5281 cccggcaggc accccctgtg actgcgccat cgagtactca cacccttttg cgctggacga
     5341 agagaagcgg ccagacggaa cggatagcgg gcgcttcctg gcggcgggcg cgaaacgggc
     5401 ctactggttg cgaactcctt ccggccagct cgggcatgcc ataccggact tgcgtaagat
     5461 tatcgaatcg agtccgaata cgatcatcga gtcaaatagc atactgcggt ttctgcgccc
     5521 ggacctgtac atcgtggtgg tggactacac cattgccgac aggaaggagt cgagttggtt
     5581 gtacatggac cgtgccgatg ctttcgtcga aattcaacgc ggcaccgggc cgcccccctc
     5641 accgtggccg gacgtcccgc acaagtggtt ggacggaaag ccacgcttcc gggtagcgcc
     5701 gcccgactac gtcccgcggg agctcgccga cttcgtcagg caacgcttga cagcgtcctg
     5761 atgcctattt tgcggacgcg cgcgcaagct gctccaggcg cttgcgaacg gagtcggcct
     5821 ctttatcgtc gggcgccagt tcgatgaatt tcttgtaggc ctggcgagcg ccttcgtcgt
     5881 ctttcaaccg gtcacgggcc tcgccgagcc gccggaatgc ctcggcaaaa ccgggattcc
     5941 acctggtcgc ttccagaaat cgcgaagagg ctgccttgaa actgcccttc ttaaagtaga
     6001 aattgcccac ctttaactct ttctcagctt gtagcggatt gaaagagtat tctttcggag
     6061 cgtaggcgac atcctcttcg ggcggttcct gggtttggac gttctctttg gattgttcgg
     6121 aactgctctg cgcggccgtg atgccgccgc tcagagagcc cagaagcaac acgaaagccc
     6181 acctgcgtgt cacaattaga gtatatcccc tcatggatct ccgagaaaaa gcgggctctt
     6241 tacccttgct tccgggagtt tatctctaca aagacggcca cggcaccgtc ttgtatgtag
     6301 gcaaggcgaa aagcctgcgg caccgggtcc gaagctattt ctccgaggac aagataggcg
     6361 acatcaaaac ggggagcctg ctgagcgaag cgacggatat cgactacata ctcgtcgata
     6421 acgagaaaga ggcgctggcg ctcgagaaca acctgatcaa gcaatggaag cctcgtttca
     6481 acatcctgct gcgcgacgac aagacctacc cgtatatcaa gctgacgaat gaaaaattcc
     6541 cgcgagtgta tgtcacacgc aggctgaaga aagacggctc cagctatttc gggccatatt
     6601 ttcccggcaa cctggctcac cgcctggtgc acttcataaa ccggcacttc aaagtgcctt
     6661 cgtgcagcgt ggacctgggc cgccgccaca cgcacccgtg cctggaatat catatccacc
     6721 gctgtctcgg gccttgtgtc gaggggttga cgaccgccga ggagtacgcg gaagccgttc
     6781 gaagcgtgcg cctctttctc gaagggcgtc accgcgacct cacccgggaa ttgcgcagac
     6841 gcatggaggc agccgcggaa gcgatgcgtt ttgaagaggc agcctcgctc cgcgacctca
     6901 tcgcgaccgt cgaggaaatg gaacagaaac agaagatggc cgcggcggaa ggcgaggaca
     6961 tcgatatata cgccttttat gccgaaccgc cgctggtcgc cgtcaacctg tttcaccttc
     7021 gcaacggccg cacggtggac cggcgggagt tcttctggga agatcaaatc gatttcgacc
     7081 cggcggagtt tttctccgct ctgctcaagc aggtgtatct ggaccagcag tatgttccgg
     7141 ggctgatcca cgtaccggtg gagttcgaag accgcgagct gctggaagag atcctttccg
     7201 aaaagcgggg acggcgggtg gagatccaca cgccacagcg cgggcgcaaa aaggcgatgc
     7261 tcggcttggt ggagacgaac gccaagagca gcttcgacca gcgcttccgt gttctgaagc
     7321 cttcgtccaa ggcgattcag ggagcgctcg aagaggcgct gaatctgccc gccgctccaa
     7381 agcggatcga gtgcttcgac atctctcaca tacaaggcac ggacaaagtg gcaagcatgg
     7441 tggtgtggga ggacggccgt atgaagaaat ccggctaccg gaaattcatc atacgcagcg
     7501 ttatcggaaa cgacgatttc gcctccatgc gcgaggtggt gacgcggcgc tacacacggt
     7561 tgaaagaaaa taaccagccc atgccggatt tagtgctcat cgacggaggg ttggggcaac
     7621 tgcacgccgc cgcggaggcg ctcgaagcca tcggcatcat caatcagccg ctggcagcca
     7681 tcgcaaaacg cgaagagatc atctacgtgt tcgggcagga ggatgaaccc gtcaagctgg
     7741 accggttctc accggtactg cacctggtac agtccatccg cgatgaggca caccgcttcg
     7801 cggttacgtt ccatcgcaca cgccgtaacg ccgcgcggct tacctcggag cttgcggaaa
     7861 tcaagggcgt gggccaggcc accatgggca aactgctgcg ccacttcggc agcgccaaac
     7921 gtgtccggca agccaccgaa cacgagttgc acgcggtggt gggacgcacg gccgcccgcc
     7981 gcatcactgc ttactacgcg cagagcgtaa catcggacga gacacagtcc gagattctcc
     8041 aatgaccaaa cggcgcgttg cggcggtcac gctcacgcta atgacactcc ttttgacaaa
     8101 cgcggcggaa gcacaacgcg aggctgatgt catcctgctg aatggcagaa tctggaccgt
     8161 taatccggct caacccgaag cggaagccgt ggcctgcggc ggcggacgta ttctcgcggt
     8221 ggggtccaat gcggagatcc gccaatggtc gggagcgaaa acgaaaacga tcgacctcgc
     8281 aggccgccgc gttgtgccgg gtttcaacga cgcacacgtg catttctaca ccgggggtac
     8341 acacctcacc agcgtgcaac tgcgaacggc acgtagcgaa gccgaattcc gggagcgcat
     8401 cgccgctttc gccgcaaagc tggagcccgg gaaatggatc accggcggcg actgggatca
     8461 cgaaagctgg actccggctc gcctgccttc gcggcaatcg attgatgctg taacaccgga
     8521 tcatcccgtg ttcgtgaacc gtttggacgg gcacatgggg ctggcgaatt ccgtggcgct
     8581 gaagatggcc ggtatcgaca gaaacacgcc ggaccccgcc gggggcagca tcgttcgcga
     8641 cgatgccgga gaacctaccg gtgttctgaa agacgccgcg atgggcgcgg tgtaccggct
     8701 gatgcctccg cccagccgcg aagatgtgct gaacgccatt cgcgccgcca tgcgctacgc
     8761 ggctgaaaac ggcgtgacga gcgttcagga catgtccgct tcgcctgaaa tcctcaaggc
     8821 ctaccaggag ctgctggcgt cggacgagtt gacggtgcgt gtttacggtc atcagcctct
     8881 gcccaattgg cagcgcctgg caaacgcggg tatccgtgcc gcgtttggag gcgacaagct
     8941 caggatcgga ggtttgaaag gcttctccga tggatcgctg gggtccacca cggcgttgtt
     9001 cttcgagccg tacctggacg caccggcaac ctcgggctta gcggccgatg aaatgattcc
     9061 gccctcgaag atgctcgaca acatttcggg agccgacgcc gcgggcttgc agatcgcggt
     9121 gcacgcgatc ggggacaagg caaactcgat ggtgctcgac ctgttcgcga gcgtggcgca
     9181 aaagaacggc gctcgcgatc gccgctttcg catcgaacat gcgcaacatc tccggaaaca
     9241 ggacatgggg cggttcggac aagcgggcgt catcgcttcc atgcagcctt accacctgat
     9301 cgatgatggc cggtgggctg agaaccgcat tggaccggag cgtgcgaaag gctcttacgc
     9361 atttcgttcg ctgctggaca acggcgctgt gttggcgttt ggatcggact ggtacgtagc
     9421 gcccatggag ccgctcatgg gaatttacgc ggcggcgacc cgcatcccgc tcgatggcaa
     9481 gaaacgggat ggatggattc ccgaggagaa aattaccgta gccgaagcgg ttcgcgccta
     9541 cacgatgggt tcggcctatg cgagcttcga agaaaaacag aagggttcca tcgaacccgg
     9601 taaactggcc gacatggccg tgctttcgga cgatattctc gccctgcccc ctgcccggat
     9661 ccgcgaggtt cgcgtcgcga tgacgatctt cgatgggcgg gtgatccaca ctcgcgaggc
     9721 gggcaagtga gccggcttat tggctacggc accgacttca ctgtgccaga tcgtcgatta
     9781 ctgcctgagt gccggaggct ttcagcaact gcctgagtct tgcccgttcg gcacgataac
     9841 cggcaattgt cgcctcaatg cgaatcgatt ccttcctggt atccggaccc caagcggagg
     9901 gtttatccgt tgtccgaaac ttgacataat ccacactaag cgtgtgctgg atgttgtctc
     9961 cgataatggg cacctgcaaa gcatcgctcc acatttcacc gggagcgtag cccagcctcg
    10021 ccgtagggcc gtctgtgctt cggatgatcg tacggtccgc tacagcgatc aacttacctg
    10081 aggcagcatc caggatgtcc agcctcagtg catacgcggc aatactgttc gctgatgtgt
    10141 tcttcaattg cagcgacagg ttcctggtgc cgtcaggatt caagattgac tgagaaacgg
    10201 tgacttgaag cggagtggaa gggtcggccg atttcatttc ttccgctcgg gtaagtatga
    10261 ctgctgccag cgcaacaatc gacgtacaga acgcggcggt gatccgcttg taagacatac
    10321 aattcctttt ttcagacact actcaataac acggcccgca gcaaggggcg tgaccaccgt
    10381 tcggatttcg ggatctgctg ggaggaaacg tagtgttcta agctgataat agaaccatga
    10441 aagaaatgac ccgccgtcaa ttcggacaga ctgctggcgt gctcaccgcc gccg
//
LOCUS       DASTMK010000056        11477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_450952, whole genome shotgun sequence.
ACCESSION   DASTMK010000056 DASTMK010000000
VERSION     DASTMK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450952"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..746)
                     /locus_tag="VFQ79_02320"
     CDS             complement(261..746)
                     /locus_tag="VFQ79_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0138511.1"
                     /translation="MAQEILDMDLNPSEETIRLGPLVVRFLVTGEDSSGSIAAFELSV
                     PGAQRLAAPAHSHDHYEETIYGIDGVLTWTVDGKPIDVGPRQALCIPRGAIHRFDNNG
                     IQDAKALCVITPAAIGPQYFREAAEVIDAAAGGPPDRTRMAEVMRRHGLTPAPPSVIA
                     S"
     gene            complement(766..1269)
                     /locus_tag="VFQ79_02325"
     CDS             complement(766..1269)
                     /locus_tag="VFQ79_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HEU0138512.1"
                     /translation="MSNQNQNQSQFKHVPAGTGPAYCGPGDRITYLITGAETGGTFFM
                     AEVSVVPGGGPPPHVHSREDESFYVQQGTLAVQVGDKALNASAGDFVHMPRGVVHSFK
                     NAGREIARLLMVATPAGLENYFAETFFPAADVADVAQIGQAVIARAMKNAPKFGLELL
                     LPTENGR"
     gene            1415..1909
                     /locus_tag="VFQ79_02330"
     CDS             1415..1909
                     /locus_tag="VFQ79_02330"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0138513.1"
                     /translation="MANARPFTIDDELKAFLEGPVSVLVGTRDSRLVPEVTRAWGPRV
                     SGDRKRVSLCVPLATSHKTLDNVEGNGAIAVAFSLPTNYRTIQLKGRRATAAEPDSID
                     LAAVERHRDVFAAVNEPLGQARDRVEAFWRVEIETSAVLVKITFAAEQVFDQTPGPGA
                     GRSL"
     gene            1906..3261
                     /locus_tag="VFQ79_02335"
     CDS             1906..3261
                     /locus_tag="VFQ79_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0138514.1"
                     /translation="MSTASPLAVALEDVASCFEGIIPASICTCSRDGTPNVTYLSIVH
                     RVDSRHVGLSYQFFNKTRENMLQNPFVQVVVVSTGNLRQYRLDLRYERTETEGAVFDR
                     MRARLDAVASQTGMSQIFKLRGVDIFEVMDCRPLNAQTGGEGLSRVDYLPQIEEFTAR
                     LAACQGLESLINVSLDALSELFGYTHSFFMVPDEDGGRLFTLGSHGFGESGIGSEVWL
                     GEGILGVAAERREVVRTTNFSRDVLYSKAVRSSVERHGEDSLLEQEIALPGLPQVESQ
                     LVVPLVSQDRLLGILCLQSAIAGRILSGDERVVRIAARHMAASMAALERTESTELHET
                     PRRIHRGQPTFTSTVKHYKADDSIFIDDAYLIKGIAGRILWKLLQSYAQRGRVDFTNR
                     EIRLDTSLRLPDIKDNLEARLILLRRRLQDRCDFVSLNPVGRGQFRLDVQRQLTLEER
                     L"
     gene            complement(3284..6571)
                     /locus_tag="VFQ79_02340"
     CDS             complement(3284..6571)
                     /locus_tag="VFQ79_02340"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU0138515.1"
                     /translation="MQKALLVAVCVAACAVGLLMYSTRDATPDLESGWRNPPPEARPH
                     AYWLWLNGYVDREAARAELQAMKDAGFGGVLLFDMGARGEKGSVPPAGPAFLSPDWLK
                     QFKESVSEAKRLGLQVDFSVVSSWDLGGYWIEPRHASMGLYASEIAVEGGREIDVELP
                     FPPIPPAAPTGADGKPAVWRNAAVLAVPSSRRGAGHEFVFLLDLTGAGDLKEAVLDQG
                     DPGNSAVTSTMTPVREFSLSLSSKSVEDADFHEVARGILRRAAGPQRFALPAGTRARY
                     ARLRFLSGYDRSRSRWTLGEFALNDVRGINVAGSHSADRARDGASLVRSSAPYGHGAF
                     WNLDNLHDGETAGPRGVYATAGAPPFQFNSAEEPINVTSLVGKDSRLRWKAPAGNWTI
                     LRYVVMNTGERLKVPSPNSDGWATDHFNPKATKVHMDYVIARLQETFGDLRSSGFRNL
                     YLASYEVTGQTWSPGFLDDFKRLRGYDMTPYLPAIFGGRIGDHETTARFLHDYRKTLS
                     DVLIGAYYVAAREAANKAGLGIKSEAGGPGPPIHNVPVDSLYANGSIDEIQGEFWPFR
                     PDAHALWVVKETASAGHLYGKPRIHMEAFTSTHHWYEGPQDLKDSADRVFCEGGNHMV
                     WHTWTHAPRDGGKPGWVYGAGSHVNTNVTWWPKIKPFIDYLSRSSFLLQHGRFVGDIL
                     FYYGDGGYKFVPPRKPQPGLGPGYDYDFINSDVILRRLAVRDGRFVLPDDTSYAVLVL
                     PDSTEAHPEVLAKLEQLVEAGGVVIGPKPQRAHGLEGFPVSDRRVREIAGKLWGDLDG
                     TSKTRRAHGKGRILWGLTPREVLASMDIAPDVIAPEGIDFIHRRDGAVDIYFVRNTVP
                     AEASATVSFRVNDREPELWDPVSGTIHPAPSWRRAAGGRIEVPFDFAAHGSMFVVFRR
                     TSRQPAPLPKSLTLPAPVALTGPWTVDFAGGPQGLVFPQLISWTAHSDPAVRAFSGAA
                     RYRTKFTMPSGWRTSGLGARLDPGRLWTVADATLNGKHLGIAWTAPFTLDASSALQDG
                     ANELVIEVVNTWHNKLVADAKLPPNQRTTRTNITVSAGKPWAELDPRESGLFGPVRIV
                     ASHDRPQ"
     gene            complement(6574..8031)
                     /locus_tag="VFQ79_02345"
     CDS             complement(6574..8031)
                     /locus_tag="VFQ79_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEU0138516.1"
                     /translation="MKVPRRKPRTARIGVFGVGYHVYWAQFSGLLEELMAKLDVLVRR
                     VEATGATVQNFDMVDKAQDAYALVPRLKAADLDLIFCDMLTYATSASFAAIIRNVDVP
                     IVLVALQPLPAMDYPRATTHIQLANDDFCSVPEFLGVAIRMGKRPPEVVLGTLHGDPT
                     AEAEIAAWCDVAMALHDVKRARIGHFGHPIEHMLDMQTDQAALTAAFGCHIVQTEADD
                     LLRLSRDISEAEIERKSTEILQLFDTPDPGADPVTTRLKTGDLRKAAHAAVALDRFVA
                     HHELDGLAYYYEGEAGSPLRELVTNLIVGNSLLTAAGFPMCGESDLKTCIAMLLMDRL
                     DIGGSFAEFHPVDFREGFVLVGHDGPHHINIAEGKPVLRSLLKYHGKPGSGASVEFCI
                     KAGPITMLSIGVKADGRFKFILAEGESVRGPIPATGNTNTRGFFGPDIRAFLKRWVYE
                     GPTHHFALGAGHHAESLRRIADALGIESAIVTKET"
     gene            complement(8028..9242)
                     /locus_tag="VFQ79_02350"
     CDS             complement(8028..9242)
                     /locus_tag="VFQ79_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0138517.1"
                     /translation="MPPRRAWLLCTLLFFATALSFLDRQVLSVLAPELTRAFHMTNAE
                     YSRVVFAFVLSYTVMFSLGGRLMDALGTRLGLALSVGLWSLASAAHALANGPWALGIS
                     RFVLGVGEGACFPAATKGAVEWMPPQQRALAVGFANGGSAFGAVLAPPLTVWWTSLFG
                     WRGAFAATALLGLLWLVAWQLAFRGLPHTAANQTPQQKTSFRTLLARPEIRRFMLARF
                     FFDPVFYFYMFWIPQYLSRERGLSLAEIGSLTWIPFFALGITNIAAGRVSDLLVRRGI
                     KPHRARMSLMLVAALLTPASWLASTAGTAGSAIALMSVLMFAHGIWIANFITLIGDTT
                     TPSEIATAVGLTGTCGGIAGMLSNLIVGPVVDQAGFTPVFLVSAVLYPAAWLILAGGR
                     RFKTPPAVKGLV"
     gene            9726..9800
                     /locus_tag="VFQ79_02355"
     tRNA            9726..9800
                     /locus_tag="VFQ79_02355"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9758..9760,aa:Asn,seq:gtt)
     gene            complement(10119..10295)
                     /locus_tag="VFQ79_02360"
     CDS             complement(10119..10295)
                     /locus_tag="VFQ79_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138518.1"
                     /translation="MRALCLCEVNLPDGWIGEDQAHECIAEDSGDLADGVFERGIVIA
                     HERSPLETTHDDLR"
     gene            10315..10839
                     /locus_tag="VFQ79_02365"
     CDS             10315..10839
                     /locus_tag="VFQ79_02365"
                     /inference="COORDINATES: protein motif:HMM:NF022598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HEU0138519.1"
                     /translation="MKTETQIREEIEGLRSFTTAQLKAKYREVFGEQSRSNHKQFLFR
                     RIAWRIQANAWGGLSERARQRALEIADDADLRIRAPKNFLREPVDDGRTIEARLKPSL
                     DPRLPLPGTPLIRRFQGKDIIVHVRPDGGFECNGRIYTSLSRAVTEATGTRWNGFAFY
                     GLGHRPGVKHGEQE"
     gene            10823..>11477
                     /locus_tag="VFQ79_02370"
     CDS             10823..>11477
                     /locus_tag="VFQ79_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU0138520.1"
                     /translation="MASKNNGNGNGAPATVRCAIYTRKSTDEGLNQDFNSLDAQRDSG
                     EAYVCSQTGEGWTLLPDQYDDGGFTGANMDRPALRRLLADVQAKKVDCIVVYKVDRLS
                     RSIRDFGKIMEILEKHGAMFVSVTQQFNTTTSLGRLTLNILLSFAQFEREIISERTRD
                     KQILARKRGKWTGGHLPLGYDLDDGNLVVNEEEAARVRQIFEWYLEGHSVFSIVANCE
                     "
BASE COUNT         2272 a         3344 c         3766 g         2095 t
ORIGIN      
        1 tccagccgca atcgcatcaa cccgcgaggt gtgaagcgaa agatgagcaa ctatccgttg
       61 cggccgcgaa tgccgcagtc gacaatcaaa actgacttct ccgcatctat caaaatcatt
      121 aagtgaacag tattggggct aagcccgagt attgcaagga gtcctgctca cacaaactgt
      181 agttcctgga gaacaagaaa tgccacaagg aacgaggtgt agcacccgtc agaggtctcc
      241 gctgccaggg ccggcggcgc ttaagacgct atgactgagg gtggagccgg agtgaggccg
      301 tggcggcgca tgacctcggc catcctggtt cgatctggcg gcccgccggc ggccgcgtcg
      361 atcacctcgg cggcctcgcg gaaatactgc ggaccaattg cggccggcgt gatcacgcag
      421 agcgctttcg catcctggat tccgttgtta tcgaaccggt gaatcgcacc tcgtggaatg
      481 cagagcgcct gccttggccc cacgtcaatt ggttttccgt cgaccgtcca ggtcagaact
      541 ccgtcgatgc catagatcgt ctcttcgtaa tggtcgtggc tgtgcgccgg cgccgccagt
      601 cgctgagcac ctggaacaga gagctcaaag gccgcgatgc tgccactcga gtcttctccg
      661 gtgaccagga agcggactac cagaggcccg agacggatgg tctcttcgga aggattgagg
      721 tccatgtcta agatctcctg tgccatgcag ttccttcttg tgagtctacc gcccgttttc
      781 agtggggaga agcaattcca aaccgaactt gggagcattc ttcatcgcac gagcaatcac
      841 tgcttgaccg atttgggcaa cgtcggcgac gtcggcagcc gggaagaaag tctccgcgaa
      901 gtagttttcc agtcccgccg gcgtcgccac catcagcagc cgcgcgattt cccggccggc
      961 gttcttaaag gaatgcacta cgccacgagg catgtgaaca aaatctccgg ccgacgcatt
     1021 cagtgccttg tcacccactt gcacagccag cgttccctgc tgcacataga acgattcatc
     1081 ctcccggctg tgtacgtgcg gtggcgggcc tcctccgggg actacactta cctcggccat
     1141 gaaaaaggtt ccgccggtct ccgcgcccgt gatgaggtag gtaatccggt cccctggacc
     1201 gcagtaggca ggccccgttc cagccggtac atgcttgaac tggctttggt tctggttttg
     1261 gttcgacatg atgttttgag cctgctttct aaggcagaat cagaatatcg ggaatcgaac
     1321 gggcgcgttc ctaaatgatc gctagaggat tcctaaatat tggctaagcg gcggctgcag
     1381 ggttccggag acgtgcgata gtctgaatct gagcatggca aacgcccgcc cgttcacgat
     1441 cgacgatgaa ctgaaggctt ttctggaagg accggtatcc gttctggtcg gcacgcgtga
     1501 ctcgcgcctc gtcccggaag tcacgcgcgc ctggggtccg cgggtttccg gggaccgcaa
     1561 gcgcgttagc ctgtgcgtgc cgcttgcaac cagccacaag acactggaca atgtcgaagg
     1621 caatggggcg atcgcggtcg ccttttcgct gcccacgaac taccggacga ttcagttgaa
     1681 agggcggcgc gccacggctg ccgagccgga tagtatcgat ttggccgcgg tcgaacggca
     1741 tcgtgatgtg tttgcggcgg tcaacgagcc cctggggcaa gctcgcgacc gtgtcgaggc
     1801 tttctggcga gtggagattg agacgtccgc ggtgctggtc aaaattactt tcgccgcaga
     1861 gcaggtgttc gaccagactc ccggtccggg cgcgggcaga agcctatgag tacggcctcg
     1921 ccactggcgg ttgcgctcga ggacgttgcg tcctgctttg agggcatcat accggcgtcc
     1981 atttgcacgt gctcccggga tggaacgccg aacgtgacat acctttcgat tgtccaccgc
     2041 gtcgatagcc gccatgtcgg tctgtcctac cagttcttca acaagacgcg ggaaaacatg
     2101 ctgcagaacc ctttcgtgca ggtggtggtc gtctctacag gcaacttgcg tcaataccgg
     2161 ctggatttgc gttatgaacg gacggaaacc gaaggcgcag tcttcgatcg catgagagcg
     2221 cggctcgacg ccgtggcgtc acaaacgggt atgagccaga ttttcaagct gcgcggtgtg
     2281 gacatctttg aggttatgga ctgtcggccg ctaaacgcgc agaccggtgg agaaggactg
     2341 tcgagagttg actaccttcc acagattgaa gagttcacgg cgcggctcgc cgcctgtcag
     2401 gggcttgaat cgctgatcaa cgtctcactc gacgccttgt cggagctttt cggctatacc
     2461 cactcattct tcatggttcc ggatgaagat ggcgggcgcc tgttcacact gggaagccac
     2521 ggcttcggtg agtccggaat cgggtcggaa gtgtggctcg gtgaaggaat cctcggcgtg
     2581 gcggccgagc gccgggaagt ggtccgaacc acgaacttca gccgggacgt gttgtactcg
     2641 aaggccgtgc gatcctcggt ggagcgtcat ggcgaagaca gcctgcttga acaggagatt
     2701 gctttgcctg ggctgccgca ggtcgaaagc cagttggtag taccgctagt gtcccaggac
     2761 agactcctcg gcatactctg tctccagagt gcgattgccg ggcgaattct gtccggcgat
     2821 gagcgggtgg taagaatcgc tgcgcggcac atggccgcct cgatggccgc gctggagcgg
     2881 acggagagta ccgaactgca cgagactccg cgacggatac atagagggca gccgacattc
     2941 acctcaacgg tcaagcacta caaagctgac gacagcatct ttatcgacga cgcgtatctg
     3001 atcaagggca tcgccggccg gatcttatgg aagctcctcc agagctacgc ccagaggggg
     3061 cgggtggact ttacgaacag ggaaatccgt ctggatacga gcttgcggct tccagacatc
     3121 aaagataact tggaagcgcg tttgatcctg ctccgacgcc gcttgcagga ccgctgtgac
     3181 tttgtcagtc tgaacccggt tggtcgcggg caattccgtc tggacgtaca gcgccaactc
     3241 acgttggagg agcggttgta ggcaggggcg gcaaacaaag acgctattgg ggccggtcgt
     3301 gtgaggcgac aatgcgaacc gggccgaaga ggccggattc gcggggatcg agttcggccc
     3361 agggcttgcc tgcggagacg gtgatgttcg tacgggtcgt gcgttggttc ggcggtagct
     3421 tggcatcggc tacgagtttg ttgtgccagg tattgacgac ttcaatgaca agttcattcg
     3481 caccatcctg aagggccgat gatgcgtcga gcgtgaatgg agcggtccag gcgatgccga
     3541 gatgctttcc gttaagggtg gcatcggcga cagtccagag gcggccggga tcgaggcggg
     3601 cgccgagtcc tgaggtgcgc catccagagg gcatggtgaa tttcgtgcga tagcgtgcgg
     3661 cgcctgaaaa agcgcggacg gcgggatcgg agtgagcggt ccaggagatg agttgcggaa
     3721 aaacgagtcc ctgcgggccg cctgcgaagt ccaccgtcca gggtccagta agagcaacgg
     3781 gtgcaggcag agtgagcgac ttagggagtg gggctggctg tctcgacgtc cggcggaaga
     3841 cgacgaacat cgagccatgc gcggcaaaat cgaagggcac ctcgatgcgg ccgccggcgg
     3901 cacggcgcca cgacggagcc gggtggatcg tgccggagac cggatcccag agttccggtt
     3961 cacgatcgtt cacgcggaaa ctgacggtgg cggaggcctc cgcggggacg gtgttgcgga
     4021 cgaagtaaat gtccactgcg ccatcgcgac gatgaatgaa atcgatgcct tcaggcgcaa
     4081 tgacgtcagg ggcgatgtcc atggaagcga gaacctcgcg cggcgtgaga ccccaaagaa
     4141 tacggccctt tccgtgagcg cgtctggttt tcgacgtgcc gtcaagatcg ccccagagtt
     4201 taccggcaat ctcgcgcact cgccggtcgc tcaccggaaa tccttccagg ccatgggcac
     4261 gctgtggttt gggaccgatg acaacaccgc ccgcctccac cagttgttcg agtttcgcga
     4321 gcacttcggg atgggcttct gtggagtccg gcaggacgag tacggcatag ctggtgtcgt
     4381 cgggcagcac gaagcggccg tcacggacgg cgagacggcg aaggatcaca tctgagttga
     4441 tgaagtcata gtcgtagccg gggccgaggc caggctgcgg tttgcgcgga ggcacgaatt
     4501 tgtagcctcc gtcgccatag tagaaaagga tgtcacccac aaaccggccg tgctgaagca
     4561 ggaacgacga gcgcgatagg tagtcgatga agggtttgat tttgggccac caggtgacgt
     4621 tggtattgac atgcgagccc gcgccataca cccagcctgg cttgccgccg tcgcgcggag
     4681 cgtgggtcca ggtgtgccac accatgtggt tgccgccctc gcaaaagacg cggtcggcgc
     4741 tgtccttcag atcctgcggg ccctcgtacc agtgatgagt ggaagtaaag gcctccatgt
     4801 ggatgcgagg tttgccgtaa agatggccgg cggaggctgt ctctttcacc acccaaagtg
     4861 catgggcatc gggccggaaa ggccagaatt cgccctgaat ttcatcgata gagccattgg
     4921 cgtagagaga atcgacgggg acattgtgga tgggcggacc gggtccgccg gcttcggact
     4981 tgatgccgag gccggccttg ttggcggctt cgcgggccgc gacatagtag gcgccgatca
     5041 ggacatcgct cagggtcttg cggtaatcgt gcaggaaccg ggccgtagtt tcgtggtcgc
     5101 cgatgcgccc gccgaagatg gcgggcagat acggcgtcat gtcatagccg cggaggcgct
     5161 tgaagtcgtc gagaaagcca ggagaccaag tctggccggt cacctcgtag ctggcgaggt
     5221 agaggttgcg gaacccgctc gagcggaggt cgccgaacgt ttcctgaagc cgggcgataa
     5281 cgtaatccat gtggaccttg gtggctttgg gattgaaatg gtcagtcgcc caaccatcgg
     5341 agttggggct ggggaccttc agccgttcgc cggtattcat cacgacgtag cgaaggatgg
     5401 tccagttgcc ggcgggagct ttccagcgca aacggctgtc ctttccaacg agagaagtga
     5461 cgttaatcgg ttcttcggcg gaattgaact ggaagggcgg cgctccggcg gtggcataaa
     5521 caccgcgcgg ccctgcggtc tcgccgtcat gcaggttgtc gagattccag aaggcgccat
     5581 ggccataggg agcggaagag cgcaccaggc tggcaccgtc gcgggcgcgg tcggccgagt
     5641 gagagccggc gacgttgatg ccgcgaacgt cgttgagagc gaactcgcct agagtccaac
     5701 gtgagcgcga cctgtcgtag ccgcttagga aacggaggcg tgcatagcgg gcccgcgtac
     5761 cggcgggcaa cgcgaaccgc tgcggccccg ccgcgcgacg caatattccg cgggccactt
     5821 cgtggaagtc cgcatcctcc acacttttcg aagagagcga gagactgaac tcgcgaacgg
     5881 gagtcattgt ggaggtgacg gcggaattac ctggatcgcc ctggtcgagc acggcctcct
     5941 tcaggtcacc ggcgccggtc agatcgagca ggaagacgaa ctcgtgcccg gcaccgcgcc
     6001 gggaactggg caccgcgagc acggcggcgt tacgccacac ggcgggctta ccgtcggcac
     6061 ccgtaggagc ggctggtgga atgggaggga aggggagttc gacatcgatc tcgcggcctc
     6121 cctcgaccgc gatttcggac gcatagagtc ccatgctggc gtggcgcggt tcaatccagt
     6181 agcctcccag gtcccaacta cttacgacag agaagtcgac ctgtaggccg agacgtttgg
     6241 cctcgctgac ggattccttg aactgcttca gccaatcggg gctgaggaag gcgggccccg
     6301 ctgggggcac agagcccttc tctccacggg cacccatgtc gaataagagc acgccgccga
     6361 agccggcgtc cttcatggcc tggagttcgg cgcgggctgc ctcccggtcg acgtaaccat
     6421 tgagccagag ccagtaagcg tgggggcgcg cttcgggagg cggattgcgc cagccgcttt
     6481 cgagatccgg cgtcgcatcc cgggtggagt acatcagcaa gcccacggcg cacgcggcca
     6541 cacaaacggc gacgagcaag gccttctgca tgatcacgtc tccttcgtga cgatggcact
     6601 ctcaatgccc agtgcatcgg cgatacggcg cagtgattcg gcatgatggc ccgcgccgag
     6661 tgcgaaatga tgcgtgggtc cttcgtagac ccatcgcttg aggaacgcgc ggatgtccgg
     6721 gccgaagaag ccgcgtgtgt tggtattgcc cgtggcggga ataggtccgc ggacactttc
     6781 gccttcggcc aggataaatt tgaaccggcc atcggccttg accccgatac tcagcatggt
     6841 gataggaccg gccttgatgc agaactccac gctggcgccg ctgccgggtt tgccgtggta
     6901 tttcagcagg gaccgcagca cgggcttgcc ctcggcaatg ttgatgtggt ggggcccgtc
     6961 atggcccacc agaacaaagc cctcgcggaa atcgacgggg tggaactcgg caaagctgcc
     7021 gccgatgtcg aggcggtcca tcagcagcat agcgatgcag gtcttcaggt cggactcgcc
     7081 gcacatgggg aagccggcgg cggtgagcag agagttgccg acgatgaggt tggtgacgag
     7141 ttcgcgcaaa gggctgccgg cttccccttc atagtagtag gcgaggccgt cgagttcgtg
     7201 atgggcgacg aagcggtcca gcgcgacggc ggcgtgcgcg gctttgcgta gatccccggt
     7261 cttcagacgg gtggtaacgg gatcggcgcc ggggtccggc gtatcgaaga gttggagaat
     7321 ctcggtggat ttgcgctcga tctcggcctc gctaatgtca cggctcaagc ggagcaggtc
     7381 gtcggcttcg gtctggacga tgtggcagcc gaaggcggcg gtgagagctg cctggtcggt
     7441 ctgcatgtcg agcatgtgct cgatgggatg gccgaagtgg ccgatgcggg cgcgcttgac
     7501 gtcgtgcagg gccatcgcga cgtcgcacca ggccgcgatt tcagcttccg cagtgggatc
     7561 gccgtgtaga gtgccgagaa ccacttccgg cgggcgcttg cccatgcgga tggcgacacc
     7621 gagaaactcg ggcaccgagc agaaatcgtc gtttgccagt tgaatgtgtg tcgtggcgcg
     7681 gggatagtcc atcgccggaa gcggctgcag cgccacaagc acaatcggga catcgacgtt
     7741 gcgaatgatc gcagcaaagc tggccgatgt ggcgtaagtc agcatgtcgc agaagatgag
     7801 atcgaggtcc gccgccttga ggcgagggac gagggcgtag gcgtcctgcg ccttgtccac
     7861 catgtcaaag ttctggaccg tggctccggt cgcctcgacg cggcggacga ggacatcgag
     7921 cttggccatc aattcttcga gcagtcccga gaactgtgcc cagtaaacgt gataaccgac
     7981 gccgaatact ccgattcggg cggtgcgtgg cttcctgcgg ggaactttca taccagtcct
     8041 ttgacagcgg gaggcgtctt gaagcggcgt cctcccgcca gtatcaacca tgccgccggg
     8101 tagagaacgg ccgagaccag gaagaccggc gtaaagcccg cctggtccac tacgggccct
     8161 acgatcaggt tggagagcat accggcgatg ccgccgcagg tcccggtgag gcccaccgcc
     8221 gtggcaattt ccgaaggcgt tgttgtgtcg ccgatgagcg tgatgaagtt ggcgatccag
     8281 atgccatgcg cgaacatgag caccgacatc agcgcaatcg cggagccggc agtgccggca
     8341 gtggaggcta gccatgaggc cggcgtcagc agcgccgcga ccagcatgag cgacatgcgc
     8401 gccctatggg gcttgatgcc gcggcgcacc agaaggtcgg agacacgccc ggcggcaatg
     8461 ttcgtgatgc ctagggcaaa gaacgggatc cacgtcaggc tgccgatctc cgcgagggaa
     8521 aggccgcgct cgcgggatag atactgcggg atccagaaca tgtagaagta aaagacagga
     8581 tcaaagaaaa atcgtgcgag catgaagcgg cggatttcgg gcctcgcaag cagagtccgg
     8641 aaactcgttt tctgctgtgg cgtctggttt gcggccgtgt gcggaagtcc gcgaaaggcg
     8701 agttgccagg ccaccagcca gagcaacccc agcagggccg tagcggcgaa ggcgccacgc
     8761 caaccgaaca gggaggtcca ccacactgtg agcggcggcg ccagcaccgc gccgaaagcg
     8821 gacccgccat ttgcgaagcc caccgcgaga gcgcgctgtt gcggcggcat ccactccacc
     8881 gcgcccttgg tagcagcggg aaagcaggcg ccctccccca cgccaaggac gaagcgcgag
     8941 atccccagcg cccacggtcc gttggcgagt gcatgtgcgg cgctggccag cgaccaaagc
     9001 cccacggaaa gggcgagccc caggcgcgtg ccgagagcgt ccatcagccg cccgccgagc
     9061 gagaacatca ccgtgtagct gagcacgaaa gcgaacacga cgcgcgagta ctccgcgttg
     9121 gtcatgtgaa aggcccgcgt aagctccggt gccaggacgc tgagtacctg gcggtccagg
     9181 aagctcagcg ccgtagcgaa gaacagtagt gtgcacagca gccatgctcg gcgcggcggc
     9241 atcgtggtca gaacatgaat cgtagcgcaa actgcgtgat gtgcggtggc tgcgatgaaa
     9301 cccgcggcta ccctgggctc ggagcgccgc tcccagaaat tctgaaagta cccgcttagc
     9361 cgacgttgac cgggtccgta cagcagcccg gcgagttgct cagatgctcc ttggcgtgaa
     9421 acttatccac gagcgccagg tgaacgagcg aatctgcgac gcgcggctgc agcccgaggc
     9481 cgaggataaa aaaacccgcg cccggtgcgg agcttcgcag tgaagtggcc gaagaacagc
     9541 gttttcgaca actttgatcc gctgcaactc tcgttgactc aacttcacct cccagattgc
     9601 cagcggggga cagagtttcg tcgcagttac ggggtgacat tattttggag catcttcacg
     9661 ataaattctg caagttgaca cgcccgaaac acgtttgttg taataagata gttgcttctc
     9721 agcgttcctc agtagctcaa tggtagagca ttcggctgtt aaccgaaggg ttgtaggttc
     9781 gagtcctacc tgaggagcca attctttttt cagcaccttc ccccgctcgt ctgttaacgc
     9841 ctctctccgt ccgcaacctc gcaactggcc gtgttttcgg cacttccgcg tctctccgat
     9901 ttgggcagcg tcccgcctga tgcgccagcc gtgcgcccgg gcgacgtttg ccggaggccg
     9961 tttctccccg tctccggaag gaacccaggt gttggttaac ggcgcggcgc gggttgtccg
    10021 gactcgaccc ggacgactcg cacccgagcg gcgtatatag ttaacggacg cggcagaacg
    10081 ccgcgccgac tacatccacc agacgccggc tcgaccgcct agcggagatc gtcgtgggtg
    10141 gtttccaaag gagaccgctc atgcgcgatc acgatgccgc gctcgaaaac gccatccgcg
    10201 aggtcgccgc tatcctcggc gatgcactcg tgcgcctgat cttcaccgat ccatccgtcc
    10261 ggcaagttga cttcacagag acagagagcc ctcatgtgac tgctggttaa cgccatgaag
    10321 acagaaaccc aaattcgcga ggagatcgag ggcctccgca gcttcaccac ggcgcagctc
    10381 aaggcgaagt accgcgaggt cttcggtgag cagtcccgat ccaatcacaa gcagttcctg
    10441 ttccgccgca tcgcctggcg catccaggcg aacgcgtggg gcggcctctc cgagcgcgcc
    10501 cgccaacgtg cgcttgagat tgcggacgac gccgatcttc gcatccgggc gcccaagaac
    10561 ttcctgcggg aaccggtcga cgacggccgg acgatcgagg cgcggctcaa accgtcgctc
    10621 gatccgcggc tgccgttgcc cggcactccg ctcatccgtc gtttccaggg caaggacatc
    10681 atcgtccacg tccgcccgga tggcggattc gagtgcaacg ggaggatcta cacgtcgctg
    10741 agccgggcgg tcacggaggc gacgggtacg cgctggaatg gcttcgcctt ttatggattg
    10801 gggcatcggc ctggggtgaa gcatggcgag caagaataac ggtaacggga acggcgcgcc
    10861 cgcgacggtc cggtgcgcca tctacacccg gaaatcgacg gatgaggggc tgaatcagga
    10921 tttcaactct ctcgacgcgc agagggactc cggcgaggcg tacgtctgca gccagaccgg
    10981 cgagggatgg acgctgctgc cggatcagta cgatgatggc gggttcaccg gcgccaacat
    11041 ggaccgcccg gcgttgcgcc gcctgctcgc cgacgtccag gcgaagaagg tcgattgcat
    11101 cgtggtctac aaggtggacc ggctcagtcg gtccatccgt gacttcggga agatcatgga
    11161 gatcctggag aagcacggcg cgatgtttgt gtcggtcacg cagcagttca atacgaccac
    11221 gtcgctcggc cgcctgactc tgaacatcct gctctccttc gcgcaattcg aacgcgagat
    11281 catctccgaa cggacgcgcg ataaacagat cctggcgcgc aagcgcggca aatggaccgg
    11341 cggtcacctg ccactcggct acgatctgga tgacggcaac ctcgtcgtca acgaggagga
    11401 ggccgcgcgc gtacgccaga tcttcgagtg gtacctcgag ggccacagcg tcttcagcat
    11461 cgtggccaat tgcgaga
//
LOCUS       DASTMK010000057         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_67318, whole genome shotgun sequence.
ACCESSION   DASTMK010000057 DASTMK010000000
VERSION     DASTMK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67318"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2183
                     /locus_tag="VFQ79_02375"
     CDS             <1..2183
                     /locus_tag="VFQ79_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138521.1"
                     /translation="PVRATAPGALPSNNWDFLTPVPSVDNKGDGRIDHYFSPKLNAFA
                     RYSHRLLNRTENHVIPGPSGGDANGNVRVFNQQVAGGFNYNLSPTSLIDFRLAVSVFE
                     GGKFALGSNRPNMAEEYGIPGLPDNPVIGGGLTRQAVSGFTAFGRQSSNPQFQNPTTI
                     NPKVNYSKIAGRHSLKMGYEYQAINTDIFDFNPQYGEDGYSGQFSRPEGVASNNLFNL
                     VDFVFGARSSYSLNNQIILNYRQRMHFGYLQDDWKVNSKFTLNLGVRYEFATPQWEDK
                     NRISSYDPVANALIRASDGGIYERTLVHPDRNNWAPRVGLAYQVTPRTVVRSGYGISY
                     VHFNRLGGENLLGYNGPSIVNLTINQIAAQPLCTGDNYRNCFRLTQQGYPAGLVDPAN
                     FSTATTRTNYTPADYRTSYVQSWHLTVQREVVKDLVVDVAYIGNRSNGLMILGDYNQA
                     RPNNPGENLTIAQRRPISGFDYIQISYGGGFAAYHGFQAKIEKRYSAGVYLLNSFTWS
                     KAIDNASGHLEAFNGDNSRVNFRNVGAEKGLGAYDQPFNNTTSVVWELPYGRGRRWGS
                     NTPTLLDAVLGGWRLTGINTMTSGQPINITYSPTAAGQVSGAPSYRPIYLGGDIYSAD
                     KSAPNYLNRAAFALPGISDPFGNLGRNVARTESIFNFDLGLHKEFPLWSESSRLQFRS
                     EFFNLFNTTNLGSPAQNVSNANFGVITGLSSPARQIQFALKLVF"
     gene            complement(2204..>4444)
                     /locus_tag="VFQ79_02380"
     CDS             complement(2204..>4444)
                     /locus_tag="VFQ79_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEU0138522.1"
                     /translation="GSRHAEYTCPMDPEVRQLGPGACPKCGMALEPVTVALPQTRTQY
                     TCPMHPEIVRDEPGNCPICGMALEPRSIVIEEDNPELRDMTRRFWISAALTLPILALM
                     VSEMLPGKPLQMLLSPAALTWGQFLLATPVALWGGWPFFVRGWQSVVNRHLNMFTLIA
                     LGTGAAFFYSVLATLMPGLFPESFRDPHTGAIAVYFEPAAVIVTLVLLGQVLELRARS
                     RTSSALKALLGLAPRMARLIQADGQEEDVAIEQVKAGDRLRIRPGEKVPVDGVVREGT
                     SYIDESMVTGESVPVEKEPGSKLTGGTVNGTGSLIMEAERVGADTLLAQIVRLVGEAQ
                     RSRAPIQRLADKVAGWFVPAVIAIAVLSAIVWGTWGPEPRLAYALVNAVAVLIIACPC
                     ALGLATPMSIMVGTGRGAQAGVLVRNAEALEAMEKVDTLVVDKTGTLTEGKPKLVTVR
                     PAPGMSDNDLLRLAASLEQGSEHPLASAIVKDAKERGLALAGVDRFQSVTGKGVRGTV
                     EGHDIAAGNARLLTEDLLLNPGALLEEAEALRREGQTVIFVAVDGAPAGLLGIADPIK
                     STTAEAIDALHRQGMRVVMLTGDNRTTAEAVARQLGIDEVEADVLPEQKAAAVKRLQA
                     ERHRVAMAGDGVNDAPALAQADVGIAMGTGTDVAMESAGMTLVKGDLRGIVRARRLSQ
                     GTMRNIRQNLFFAFVYNALGVPVAAGLLYPFFGLLLSPMIASAAMTFSSVSVIGNALR
                     LRRLEL"
BASE COUNT          853 a         1445 c         1290 g          856 t
ORIGIN      
        1 caccggttcg cgccaccgcg ccgggcgctc ttccttccaa caattgggac ttcctcactc
       61 cggtgccgtc ggtcgacaat aaaggcgacg ggcgcatcga tcactatttt tccccgaagt
      121 tgaatgcgtt cgcccgctac agccaccggc tgctgaaccg cacggagaac cacgttattc
      181 caggaccttc cggcggcgat gcaaacggca atgtgcgcgt gttcaaccag caggtggccg
      241 gaggttttaa ttacaatctc tcgccgacgt ctttgatcga cttccgcctg gccgtatctg
      301 tatttgaagg tggaaaattc gcgctcggct ccaaccgtcc gaatatggcg gaggagtatg
      361 ggattcccgg actgccggac aacccggtaa tcggcggagg gctcacccgt caggcggtca
      421 gcggtttcac tgcattcggc cggcagtcct cgaacccgca gttccagaac ccgaccacaa
      481 tcaacccgaa ggtgaactac tcgaagatcg ccggccggca ttcgttgaaa atgggctacg
      541 agtatcaggc gatcaacacc gacattttcg acttcaaccc gcagtacgga gaggacggct
      601 attcgggcca gttctcccgt ccggagggag tagcctccaa caacttgttc aacctggtcg
      661 attttgtttt cggcgcgcgg tcttcgtaca gtttgaataa tcagatcatt ctgaattacc
      721 gccagcgcat gcatttcggc tatctccagg acgactggaa ggtgaactcg aagttcacgc
      781 tgaatcttgg ggtgcgctac gaattcgcta cgccgcagtg ggaagataag aaccgtattt
      841 caagctatga tccggtggcg aacgcgctca tccgcgccag cgacggcggc atctacgagc
      901 ggacgctcgt gcatcccgac cggaataact gggcgccgcg tgtcgggctc gcctaccagg
      961 tgacgccccg gacggttgtg cggtccggct acggcatcag ctacgttcac ttcaaccgtc
     1021 tcggcgggga aaacctgctc ggctacaacg gccccagcat cgtgaacctg accatcaatc
     1081 agatcgccgc gcagccgctc tgcaccgggg acaactaccg caattgcttc cgcctgacac
     1141 aacagggcta tccggccgga ctggtggacc cggcgaactt ctcgacggcg accacgcgca
     1201 ccaattacac gccggccgac tatcgcactt cctatgttca gagctggcac ctcaccgtgc
     1261 agcgcgaggt tgtgaaagac cttgtcgtcg atgtcgccta tatcggcaac cgctccaacg
     1321 ggctgatgat tcttggagac tataaccagg ctcgcccgaa caatcccggt gaaaacctga
     1381 ccatcgcgca gcgccggcct atttccggct tcgactacat ccagatctcc tacggcggcg
     1441 gcttcgcggc gtaccacggt tttcaagcca agatcgagaa gcgctattcg gcgggagtat
     1501 atttgctgaa cagcttcacg tggtccaaag cgatcgacaa tgcctccggg cacctggaag
     1561 cgttcaacgg cgacaactcc cgcgtgaact tccggaacgt cggtgcggag aaaggtctcg
     1621 gcgcttacga tcaaccgttc aacaatacga cgagcgtggt gtgggagctt ccgtacggcc
     1681 gggggcgccg ctggggatcg aatacgccta cccttttgga tgccgtgctc ggtggatggc
     1741 ggctgaccgg aatcaacaca atgacgagtg ggcagcccat caacattacc tattcgccca
     1801 ccgcggcggg ccaggtatcg ggcgctccga gctatcggcc gatttacctc ggcggcgaca
     1861 tctactccgc ggacaagagc gcgccgaact acttaaaccg ggccgcgttc gctctgcccg
     1921 gcatttccga tccgttcggc aatctgggca ggaacgtcgc acgcaccgaa tcgattttta
     1981 acttcgattt ggggctgcat aaggaatttc cgctctggtc ggagtcttcc cggctacagt
     2041 tccgttccga gttcttcaac ctgtttaata cgaccaacct cggctcgccg gcccagaacg
     2101 tcagcaacgc caacttcggc gttattacgg gactttcgag cccggcgcgg cagatccagt
     2161 tcgcgctgaa acttgtgttt tgaccggaat tgcgccggct cgcttataat tcgagccggc
     2221 gcaaccggag ggcgttgccg atgaccgaca cggagctgaa cgtcatcgcg gcgctggcga
     2281 tcatcggact cagaagcaag ccgaaaaacg ggtagagtag accggctgcc accggaactc
     2341 cgagtgcgtt gtacacgaac gcgaagaaca ggttctgccg gatgttccgc atggttccct
     2401 ggctgaggcg tcgcgcccgc acaatcccgc gaagatcgcc ctttaccagc gtcattcccg
     2461 cactctccat cgctacgtcc gtaccggtgc ccatggcgat accaacatcg gcttgcgcca
     2521 gcgccggcgc gtcgttgaca ccatctcccg ccattgccac tctgtgccgt tccgcctgaa
     2581 ggcgcttcac agcggccgcc ttctgttccg gtaagacatc ggcctccact tcatcgatac
     2641 caagttggcg cgcgacggcc tccgccgttg tacggttgtc gccggtgagc atcacgacac
     2701 gcatgccctg ccgatgaagg gcatcgattg cctccgcggt ggttgacttg attggatcgg
     2761 cgataccgag caggcctgcc ggggcgccgt ccacggcaac gaatatcacg gtctgcccct
     2821 cacgccggag cgcctccgcc tcttcgagca gcgcgccagg attgaggagg aggtcctccg
     2881 tgaggagccg cgcattgccg gcggcgatgt cgtgtccctc caccgttcct ctcacgcctt
     2941 tccccgttac cgactgaaag cggtccacac ccgccagtgc gaggccgcgc tccttggcgt
     3001 cttttacgat ggccgacgcc agcgggtgct cgctcccttg ctccaggctc gccgccagcc
     3061 ggagaagatc gttgtcactc atcccggggg ccggacgcac ggttacgagc ttcggcttgc
     3121 cctccgtcaa agtgccggtc ttgtccacca ccagcgtatc tactttctcc attgcctcca
     3181 gggcttcggc gtttcgcacc agcacccccg cctgcgcgcc gcgtccggtg cccaccatga
     3241 tggacatggg agtagcaagg ccgagcgcgc acgggcacgc aataatcaat acggcgaccg
     3301 cgttcacaag agcataggcc agtctgggct caggccccca ggtaccccag acgattgccg
     3361 agagaaccgc aatggcgatc acggcgggca cgaaccagcc cgctaccttg tcggcaagcc
     3421 gctgtatggg cgcgcgagaa cgctgtgctt ctcccacgag ccggacgatt tgcgcgagca
     3481 gcgtatccgc gccgacacgc tccgcctcca tgatgaggct gccggttcca ttgacggttc
     3541 cgccggtgag tttgctgcca ggttcctttt caaccggcac gctttcgccg gtgaccatgg
     3601 actcgtcgat gtagcttgtg ccctcacgta ccacgccatc caccggcacc ttctcgcccg
     3661 gtctgatccg tagccggtcg cctgctttca cctgctcaat ggcgacgtct tcttcctgcc
     3721 cgtccgcctg gatgaggcgc gccattcgcg gtgcaaggcc aaggagggcc ttcagtgcgc
     3781 tggaggtccg gcttcgagcc cgcagttcga gtacctgacc gagaagcacg agcgtcacaa
     3841 tgaccgccgc cggctcaaag tagacggcaa tggccccggt atgtggatcg cggaacgatt
     3901 ccggaaacag gccgggcatc agcgtggcga gcacgctata gaaaaacgcg gctccggtgc
     3961 cgagtgcaat cagcgtgaac atattcaggt ggcggttcac taccgattgc cacccgcgca
     4021 cgaagaacgg ccagcctccc cacagcgcga caggcgtggc caacaggaac tgtccccacg
     4081 ttagggccgc cgggctcaac agcatttgaa ggggtttgcc gggcagcatc tcggagacca
     4141 taagcgcgag gatcgggaga gtgagcgccg ccgaaatcca aaagcggcgc gtcatgtccc
     4201 ggagctccgg gttgtcttct tcgatcacga tcgaacgcgg ctcgagagcc ataccgcaga
     4261 tggggcaatt tcccggctcg tcccgcacaa tctccgggtg catcgggcag gtgtattgcg
     4321 tgcgcgtttg tggcagcgcg acggtaacag gctccagtgc catgccgcac ttcggacaag
     4381 cgccggggcc aagttgacgc acctccgggt ccatcggaca ggtgtattcg gcgtgccggc
     4441 ttcc
//
LOCUS       DASTMK010000058         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_35195, whole genome shotgun sequence.
ACCESSION   DASTMK010000058 DASTMK010000000
VERSION     DASTMK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35195"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFQ79_02385"
     CDS             <1..198
                     /locus_tag="VFQ79_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138523.1"
                     /translation="EIGDKDGVVVLPHRFVEVEMNRVGELYRKLGLADRFLIAAFAGP
                     HKIDGHQVYPFLDRLLQWKPR"
     gene            198..3041
                     /locus_tag="VFQ79_02390"
     CDS             198..3041
                     /locus_tag="VFQ79_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138524.1"
                     /translation="MKLAILAFLACGAAPAANLVAAHDYDPVTDSVVAWKLDPGESVA
                     QSFTIAKGTKALRGFRVRIRRHGSPPPVEYRVRSALRSGELLSGSMDLKHSNPWFERW
                     YGPDFPRALPVESGATYYLELRVPKSSGGYYEVFGTSDREIANPRFLPRFRYEENWDP
                     DPNAPKQFENPANIDYGAHTPVYREGSAYDGAGAALDGFDLAFEILGDGAAVPGARDG
                     VCNRCEERFAFTEDITGPLYSKPMRDPAVRVKPSEVEVDGLWQVRNRLAAEPVVQTAV
                     DEFQEFLRTAMEVHTATAAGKRGVIQVATKAQLPDAGKGLSVPESFRLVVTDGEVLVC
                     GFDSRGAMRGLHHVEALMKLKRAPVLPLMNEIRAPKHSPRITSAPFYAKMELDTPEDA
                     YSNGLLGRMSRAGFNAIWLWGDLDEVAHSSVYPELDQGVRDRQAKLRRLIARTSRYGI
                     DVYMYLANRPLPDSFYQKHPGVQGSERRSYGGTHILCTSVPEVRQHLKAATTDLMKSA
                     PGLKGIVFIVGGEGFMHCYTRKNTCPRCSRRTPQETIAEFSRSLVEGARAANPEAAVV
                     LWPYSASNTWSGNDITQSELIGQLPPSITLMTEFGKEGEIRFGDIAIPAYDYPISFAG
                     PSKRFVKQAELASAKGMPFWVKTEHAIALEFVDTPYIPVFFQYATRFERIRSAPHLSG
                     IFANWMHYGFMPSTAADVYYWHHWSTPADTEAVLGRIAERDYGAGASYAVNAWREFSS
                     AIREYPFSSAMAMGPIQKGPSHPLFLDSRYRPLHDAGRQFKNDLSWTRPWGPALALSQ
                     LEKLEAGWARGVDLLRKAVEAAPERIRVDAKRETGVAEALLFSIRSTIHVGRFYEARE
                     LLEKEGNFVRAAEALDRMAAIARAELDNAQAALPIVCADSRIGYANSGRNDQTGVPRA
                     GIYSPGAVEKKILQLRKLLDEQIPAERVRRGLR"
     gene            complement(3033..3893)
                     /locus_tag="VFQ79_02395"
     CDS             complement(3033..3893)
                     /locus_tag="VFQ79_02395"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0138525.1"
                     /translation="MRIGFVGMGIMGAPMALNLLRAGFDLTVYNRTASKCAPLVSEGA
                     KQAESLPALAASSDVVITMVSDTPDVEAVLFGNGGLSEGLKPYSTFIDMSTIAPSAAV
                     RFAARLRNKPVDYLDAPVSGGETGAKAAKLSIMAGGDGAVFERCLSLFQAMGTSVVYT
                     GPTGSGLKTKLINQIVGALNLLGAAEGVRVCQAAGLDAGTTLQAVGSGAAGSWMLSNL
                     GPKMASGDYSAGFSIRLQQKDLRLAMEFCRELGIDAPGVALVFDLFTRALESGFGECG
                     NQGLIRLWSA"
     gene            complement(3895..>5146)
                     /locus_tag="VFQ79_02400"
     CDS             complement(3895..>5146)
                     /locus_tag="VFQ79_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138526.1"
                     /translation="MTLEEISLQLLNNLNNAMRSTGGNEKELIAWSYGDDVPWVERLI
                     PKLPKNVIVQSDFSKGGLVVRDGVRHLTGDYNLTLVGPPESYVRQHRAAKQAGLDFVT
                     KTEHAVSQEFIFVPYIPAMEQWARRIGKIRTVDTQGIFANWCHYGYMASLPAQLLNEM
                     AFDPAPDAEVELHSLAVRNYGPQSAPLVVRAWRHLSDGIREFPYSDNVSRIPGPLQKG
                     PSNPFFLDPKIPNFGRWRSWQNDLEWTKPWGPAVAAKYLAKVRAEFVKGAALLDEART
                     ASNGKYREALDAEWRIATLLESSLATVLHLIDWIEARDRFYSARSDSERTTAAHELES
                     VLIAERANVQRVLPLLEADSRLGYASEGGGVLRGGLFTPELVRWKLGQIDNVLRIELP
                     RLSGRPAAAVPSTIGEAIAGTNRN"
BASE COUNT         1040 a         1565 c         1528 g         1013 t
ORIGIN      
        1 gagattggag ataaagacgg agtagtggtg ttgccgcacc ggttcgtgga agtcgagatg
       61 aaccgggtgg gggaacttta tcgtaagctc ggactcgcag accggttcct catcgctgca
      121 ttcgcgggac cgcataagat cgatgggcac caggtctatc cttttctcga ccggttgctc
      181 cagtggaaac ctcgttgatg aagcttgcta ttcttgcctt cctggcgtgt ggggccgcgc
      241 ccgccgcgaa cctagtcgcc gcgcacgatt atgatcccgt caccgattcc gtcgtggcat
      301 ggaaactcga cccgggcgag tcggttgccc agagtttcac gattgcaaaa ggaaccaagg
      361 cgttgcgcgg cttccgggtt cgcatcaggc gtcacggcag cccgccgcct gtcgagtatc
      421 gggtccgctc cgccttgcgc tccggcgaac tactttcagg ctcgatggac ctgaagcatt
      481 ccaatccctg gtttgaacgc tggtacgggc cggattttcc gcgcgcgttg ccggtggaat
      541 ccggcgcgac atactacctg gagctgcgtg tcccgaaatc atccggaggc tactacgaag
      601 tgttcgggac atccgatcgc gaaatcgcca atccccggtt cctgccgcgc ttccggtatg
      661 aagagaactg ggaccccgat ccgaatgctc caaagcagtt cgagaacccg gcaaacatcg
      721 actacggcgc gcacacgccg gtgtaccgcg aaggttccgc ttatgacggc gccggagccg
      781 ctctcgacgg ttttgacctg gcgttcgaga tattgggcga cggcgcggcc gtgcctggcg
      841 cgcgtgacgg cgtatgcaac cgctgcgaag agcgcttcgc tttcaccgaa gacatcaccg
      901 gcccgcttta ttcaaagccg atgcgcgacc cggcagtacg cgtcaaaccc agtgaagtcg
      961 aggtcgatgg actctggcaa gtgcgaaacc ggctcgcggc ggagcctgtc gtgcaaacgg
     1021 cggtggacga gtttcaggaa tttcttcgga cggcgatgga agttcacacc gctactgcgg
     1081 cggggaagcg cggcgtgatc caggtggcaa ccaaagcgca acttcccgat gctggaaagg
     1141 gtctatccgt acccgaatct ttccggctgg tagtgaccga tggagaagtg ctcgtgtgcg
     1201 gtttcgactc ccgaggagcc atgcgcggtt tgcatcacgt ggaagcgctc atgaagttga
     1261 agcgagcgcc ggtcctgccg ttgatgaatg agatccgcgc gccgaaacat tctccacgga
     1321 ttacgtcggc tccgttctac gccaagatgg agctggatac cccggaggac gcctatagca
     1381 acggcctgct gggacgaatg tcccgcgccg gcttcaacgc gatatggctg tggggcgacc
     1441 tggatgaagt ggcgcactcc tccgtctatc cggaactcga tcaaggtgtt cgcgaccgcc
     1501 aggcgaagct gcgccggctg atcgcccgga cttcgcgtta cggaatcgac gtttacatgt
     1561 acctggcaaa ccggccgctg ccagattcgt tctatcaaaa gcaccctggc gttcaaggca
     1621 gcgagaggcg gtcgtatggc ggtactcaca tcctctgtac aagcgtgccc gaagtgcggc
     1681 agcaccttaa agcggccacc acggacctga tgaagagcgc ccccgggctg aaaggaattg
     1741 tcttcatcgt aggcggcgag ggttttatgc attgctacac gaggaagaat acgtgccctc
     1801 gctgttcccg gcgcacgccg caggaaacaa tcgccgagtt ttcgagatcg ctggtagagg
     1861 gcgcgcgcgc cgcgaatcct gaagccgcgg tggtgttgtg gccctacagc gcgtccaaca
     1921 cctggtccgg gaacgatatc acgcaatccg agctcatcgg acaactcccc ccatccatta
     1981 cgctgatgac cgagttcggc aaagagggag aaatccggtt tggtgatatc gccattcccg
     2041 cctacgatta tccgatcagc ttcgccggtc cttcgaaacg attcgtgaaa caggctgagc
     2101 ttgcatccgc gaagggaatg cccttctggg tgaagaccga gcacgcgatc gcgcttgagt
     2161 tcgtcgacac tccttatatt ccggttttct ttcaatacgc cacgcggttc gagcgcattc
     2221 gcagcgcgcc gcatctttcg ggcatattcg ccaactggat gcattacggg ttcatgcctt
     2281 ccacggctgc cgatgtctat tactggcacc actggagcac tccggcggat accgaagctg
     2341 tgctcggcag gatagcggag cgcgattacg gcgccggtgc aagttacgcc gtcaacgcat
     2401 ggcgcgaatt cagcagcgcc attcgcgagt accctttttc gtcggccatg gctatggggc
     2461 ctattcagaa aggcccgtct caccctctgt tcctggacag ccgctatcgc ccgctgcatg
     2521 atgcaggccg gcaattcaaa aatgacttga gctggacgcg tccgtgggga ccagcgctgg
     2581 cgctatcgca actcgagaag ctggaagccg ggtgggcccg cggtgtggat ctgctgcgca
     2641 aagcggtgga ggcagcgccg gagcgaatcc gcgtggacgc caaacgcgag accggtgtag
     2701 cggaagcgct tctcttctcg attcgaagca cgattcacgt gggccggttc tatgaagccc
     2761 gcgaactgct ggagaaggaa ggaaacttcg tgcgcgcggc ggaggcgctc gaccgcatgg
     2821 ctgccatcgc ccgagccgaa ctcgacaatg cgcaggctgc tcttcccatc gtgtgcgcgg
     2881 attcgcgaat cggatacgcc aacagcggaa ggaacgatca gacaggcgtg ccccgcgcgg
     2941 gcatctattc accgggcgcg gtggagaaaa agattctcca actcaggaaa ctgctcgacg
     3001 aacagatacc agccgagcgc gtacggcggg gattacgctg accagagacg gatgagaccc
     3061 tggttgccgc attccccgaa tccggattcc agcgcgcgtg taaacagatc gaagacgagc
     3121 gccacacccg gtgcatcgat ccccagttcg cggcagaatt ccatcgccag gcgcagatct
     3181 ttctgttgca gccggatgct gaacccggcg gaataatcgc cggacgccat cttcggacca
     3241 agattgctca gcatccacga acccgctgcg cctgacccga ccgcctgtaa ggtagtgccg
     3301 gcatcaagcc ccgcggcctg gcacacgcgg acaccttcgg ccgcaccgag cagattcaaa
     3361 gcgccgacaa tttgattgat caatttcgtt ttgaggccgc tgccggtggg accggtgtag
     3421 acaacgctcg ttcccattgc ttgaaacaac gataggcaac gttcgaatac cgcgccatcg
     3481 ccgcctgcca taatcgaaag tttcgctgcc tttgcgcccg tttcaccacc gctcacaggc
     3541 gcgtcaagat agtcgaccgg cttgttccgc aatcgggctg cgaagcgaac cgcggcagac
     3601 ggagcgatag tgctcatgtc gatgaaagtg gaatacggtt tcagcccttc agagagtccg
     3661 ccatttccga agagcaccgc ttccacgtcc ggcgtatcgg agaccatcgt aatgacgaca
     3721 tcgcttgaag cggcaagcgc tggaagcgat tcggcctgct tcgcaccttc gcttaccagt
     3781 ggggcgcatt tcgatgcggt gcggttgtag accgtcagat cgaaaccggc gcgcagcaga
     3841 ttcaacgcca tcggcgcgcc catgattccc attcccacaa atccgattcg catgtcagtt
     3901 cctgtttgtg ccggcgatgg cctcgccgat agtcgacggt acggccgcgg ccggccggcc
     3961 cgaaagacgg ggcaactcga ttcgaagcac gttgtcgatc tgcccgagtt tccagcgcac
     4021 caactccgga gtgaataatc cgcctcgcag aactccgccg ccctccgatg cgtacccgag
     4081 ccgcgaatcg gcttcaagca acggaagaac acgctgcacg ttcgcccgct cggcaatcag
     4141 aaccgattcg agctcatgcg cggcggtcgt gcgttcggaa tctgatcgcg cgctgtagaa
     4201 ccggtcccgg gcctcgatcc aatcgatgag atgaagcacg gtcgccaggc tgctctcgag
     4261 aagcgtcgcg atccgccatt cggcatcgag agcctctcga tacttcccat tcgaagccgt
     4321 tcgcgcttcg tccaacaaag cagcaccttt cacgaactcg gcgcgaacct ttgccaggta
     4381 ttttgccgct acagcggggc cccacggctt cgtccattcc agatcgtttt gccaggaccg
     4441 ccatcgtcca aagttcggga tcttcggatc gaggaagaac ggattgctcg gacccttttg
     4501 cagcggcccc ggaatgcgtg aaacgttatc ggaataggga aactcgcgga tgccgtcgct
     4561 cagatgccgc catgcgcgca ctaccagcgg agcgctttga gggccgtagt ttcgcacggc
     4621 caacgaatga agttccactt cggcatccgg agccggatcg aacgccatct cgtttaacag
     4681 ttgcgcgggc agcgaggcca tgtatccgta gtgacaccag ttggcgaaaa tcccttgcgt
     4741 gtcgacggtt cgaatcttcc cgatgcggcg cgcccactgc tccatcgccg gaatataagg
     4801 gacgaaaatg aattcctggc tcacggcgtg ttcggtcttg gtgacgaaat ccaggcccgc
     4861 ttgtttggcc gcccggtgct ggcgaacgta ggattcaggc gggcccacca gggtgagatt
     4921 gtaatctccg gtcaggtggc gaacgccgtc ccgcaccacg aggcctccct tgctgaaatc
     4981 gctctggaca atgacgttct tcgggagttt tggaatcaat cgttccaccc aggggacgtc
     5041 atcgccgtag ctccaagcga tcagctcctt ttcgttgccc ccagttgatc gcatcgcatt
     5101 gttcagattg ttgagcaact gaagcgagat ctcctccagc gtcata
//
LOCUS       DASTMK010000059        12863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_323222, whole genome shotgun sequence.
ACCESSION   DASTMK010000059 DASTMK010000000
VERSION     DASTMK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323222"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFQ79_02405"
     CDS             <1..554
                     /locus_tag="VFQ79_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138527.1"
                     /translation="ERPVFTWRTVEQVEFGPDVPLVLVRVQNGALVSAPERAVRKHAA
                     RSIRPHIGFGSDEIVSRADGLLRKFDVNTTDAGFTRTPYYFAKLTLEEDPRLTAAMAA
                     LLMATAFIEAPSSTGFSLCTGLSTAGAGLSIATVTWIGLEPVRGCEPLLNLQLLFNLA
                     GLFLSPFTFAIPGSIAVSHGGFS"
     gene            551..1465
                     /locus_tag="VFQ79_02410"
     CDS             551..1465
                     /locus_tag="VFQ79_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138528.1"
                     /translation="MSARTDIPSLQRIRFFSGQRLTAEDMMALQKANRELRWLHNRSL
                     HGWGIGTGFGVTGARGASSVTIEPGYAIDCMGREIILTEPAVMTIPAVAGVLSPENEL
                     SPAVYYLTASYKDDTSQNVAEKRPGVCVPGGTVRLTEEPVLAWRQPDKITEGMDIILA
                     QASILNCQLAADLSLAVRRSARPSQQPYIAAGQTPPGNTQWSAIVAGGNVVGVSTTVS
                     TSGARFQSTPQYFAHVVGSRSVSTPSFVLVVNVLPVVKDPAPDQFVCEAYVVFPGSPT
                     GGGGSTPAAVEAIVETLQWYVVWMGVEG"
     gene            1469..2749
                     /locus_tag="VFQ79_02415"
     CDS             1469..2749
                     /locus_tag="VFQ79_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU0138529.1"
                     /translation="MIEEAGYLYVNLGNEWPGVQLTPALENAGGLLRLRPAGSGFETG
                     GAFLAGPFQVSDQPVSWFRVEAFLGEPAGSPAAPVHVRFFTFSSSGPPAPWNPASESP
                     FSDPGWSAAPGNALGFHANVPPGQRFFLGGVLRGDGNASPQIEQIRIFHGRDTYARFL
                     PPIYREDAAARDFLERFLSIGQSVFGGIEDRIRDLPLLFDPYASPHGDPPSWLTWLSG
                     WLTFVLDEHWTDAQAREYLAQAFQLYGLRGTLEGLRRYLKIYAGVNAHIEEAAQQTYI
                     WSLGQAGALGFSTMLAPGPLQGAVLDASATLDQSHLTTGAEFGAALFEDVAHRFCVRV
                     YCGELTRPGLLETVRAVIDREKPAHTEYELCVIGPQMRVGIQARVGIDSIVAAGPRGA
                     ATGKPLGDGVLAASPSPEDGDQTPGVPVQQLCGE"
     gene            2771..4378
                     /locus_tag="VFQ79_02420"
     CDS             2771..4378
                     /locus_tag="VFQ79_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU0138530.1"
                     /translation="MATALLTDCSQTKMPGLERVRYFPRQLLSADDMVADQEYFRTKL
                     RRHNRFLHGWGTVCGLEVAPAPAEGRPWQVQIASGYALGPYGDEIYVRDAVLLDLAEC
                     GPGAETDPCAPGFILRGSVSRTGATLYVALRYEECLSRPVRVLPGGCGCEDTSCETSR
                     IRDSFVLECLTELPPSHTTPPGPSLCDLLRGRALAQCPPRPTDPWVVLAQVRLPADPS
                     TTIPANQIDNFIRRQVFSTAAIQDQVIRCCCGPQERRPARVTSINPPPNSRFTSGTAV
                     PAAVVITFSKNLQAQTVNTDTVQVLRFLPNQPAVFVSGQVTYDDGNRVARFTPAAPFT
                     QNGIYQVNVAGSGPNPIVDADNLALDGNDDGLPGGNFVSQFTIEAPSTTPTPTPTPTP
                     GRPLSVKVATVGDIPKVIPNNEPGSVAGLALLFTGGTPRQEVEANLMVFLNVNVATST
                     GPVVLLNQITGESMNAQIGQNNYAFPNVKFTMPATGGEQPFEINKMAVIIPRDGPNIQ
                     EVTALVSVTSATPITVENPQVTVAFVS"
     gene            4397..5080
                     /locus_tag="VFQ79_02425"
     CDS             4397..5080
                     /locus_tag="VFQ79_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138531.1"
                     /translation="MANGNVQPNRQLRAPAHADFRAGRYLRAADLQLEQAYRLERARR
                     HNRLLHEWGIVCGLWVAAAADPARPWALYVCPGFAIGPYGDEIEVRERTLVNVRDFLW
                     AKPPEGKLELRRAFVAIRYRERPRRLAALPATPCACDHPEYAASRVADGFELAALWTP
                     PKVRVPQGICERTARLCPECPESGWIVLARVLLPAAPSQLITQADIDNGVRSALSNST
                     SGSFIAERE"
     gene            5086..6150
                     /locus_tag="VFQ79_02430"
     CDS             5086..6150
                     /locus_tag="VFQ79_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138532.1"
                     /translation="MARFGEDSQAPTRLATPERNAYFFGKLLGVSDFDLEQDYFNSKR
                     WLMNRLLAGSGVACGLGVAPVVDGTRLVIDPGVAIDGWGREIIVPEQSFPFDPRVLRD
                     AEGNTSGSIEGEGTVVISLCYHECGAEPAPVLAPGCDPEGDCASSVTREKYRIVVTEG
                     VAVPSGLDCRFASLFTPAEDSDQLPNPLPALAEHITSACTEASGGGCILLAQVSLPAE
                     GGITPEMIDVRVRPVVLSHGILLELIFCLARRVQELAAGPVPTPTPTPTPEPTPTPTP
                     EPTPTPEPTPTPAPEPTPRPTAEPVPTRTPAPSPRPTLGPVPTVLPTRTPVPVPTVLP
                     TRTPTPRPAGKRVPRPTRRP"
     gene            6150..7070
                     /locus_tag="VFQ79_02435"
     CDS             6150..7070
                     /locus_tag="VFQ79_02435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0138533.1"
                     /translation="MSAEVSIIVTSRDEDPAVLQATLDGLRTTTRGVSTEIIVIDDCS
                     RAPVVCEGSGVSLVRNSRPIGVCGSRRLGAGLAGGKVLAWVDAHMSFGEHWLEQMLVH
                     AASGALLCSPFWDYDLQTCLCWGADFMWNGTRNYMEQKVPGFGLLHRVAPVRELAPEV
                     PMVIGACYMMRRDSYERMGGFSPLFRIWGIDEQDLSARAWISGAGVRCVTSAKVGHLS
                     RTAFPYPVSFDHIEFNQIVMLRTLFEPQTVRKLERWFEPIPPQVRSWLSNTGLASWRN
                     TVQTCRRLSDRQFFLRFLPGLADELGLRAA"
     gene            7210..7575
                     /locus_tag="VFQ79_02440"
     CDS             7210..7575
                     /locus_tag="VFQ79_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU0138534.1"
                     /translation="MNQQIQAIAELDRVIHEPGRLMIVALLFAVEKADFLYLQHETAM
                     NKGTLSSHISRLEEAGYVEVVKTYRGKVPQTLLRLTGAGRKAFEQYRRKLKEALSVQG
                     RQPDRPRHPRPVRIAESHA"
     gene            complement(7592..8803)
                     /locus_tag="VFQ79_02445"
     CDS             complement(7592..8803)
                     /locus_tag="VFQ79_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138535.1"
                     /translation="MSNESLIKDALCAADGIVRLAPCWVPRSFLMPGGRLKLDPRDLY
                     ALGAHRGGIDERWFSSTTRADNGPGTPDDEGLSYIALNGKRVLLKEAIETRGDEFLGA
                     GVMSKYGGWNLLCKFFDNLGPIPHHMHQKEEHAKKVGRNGKPEAYYFPPQYNFKDNNF
                     PYTFMGLEPGTTRDDIRRCLERWNEGDNGILYHSKAYRLQPGTGWQIDAGILHAPGSL
                     VTYEPQVNSDVFGMFQSMVEGRAVPWDLLVKDVPPEHHHDLDYILDMLDWEANVDPEF
                     AKHRLFHPTPVKPEAEMLDAGYSEKWIVYSTPNYSAKELTVLPGRSVTIQDAAAYGLI
                     VVQGWGSAGKQDVETPSLIRYGEMTKDELFVSARTAEVGVTVTNRSSTENLVMLKHFG
                     PGNPDAEQFIA"
     gene            complement(8906..9535)
                     /locus_tag="VFQ79_02450"
     CDS             complement(8906..9535)
                     /locus_tag="VFQ79_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU0138536.1"
                     /translation="MNRKQFVLPALVLGAAAFAYAQANTPTKVGIINIQAAIVGTKEG
                     QKAASEIEAKRAPKAKQLQGQQEEIKSLQEKLSKGSNTMAETARQGLMRDIDQKTKAF
                     NRDLEDAQMEWDQEQQKVLQELGGKMMAVINKYASDHGYTLILDISSPQTPVLYAANS
                     IEITKDIIDLYDKNAPGAATTPSASPAPATTTPPPTPPAAAKPKPGAPK"
     gene            complement(9638..12592)
                     /gene="bamA"
                     /locus_tag="VFQ79_02455"
     CDS             complement(9638..12592)
                     /gene="bamA"
                     /locus_tag="VFQ79_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU0138537.1"
                     /translation="MSFRRPKRLSIAAVCLSCLASAASPQASPQQSTAPASQPPQQQS
                     NPFETVPQAEPPKPQPPKPAQPKPGQTPFEAPKPAAEPPKTEKTPDIIEAIEFRGSRR
                     VPQDTLRAMIFTKRGDRYDEEALHRDFMALWNSGRFDDIRLEREPGNAGWIVRFLLTE
                     RRVVRSIKYEGAKSVTVSEILDRFKERRVGLSVESQYDPNRVQRATVVLKEFLAERGR
                     QFAVVEPQVRQIPPSSLEITFKVTEGPKVKVGDIDIEGNTVFGDRAVIRSMKNLRPIG
                     IPHSILFEGLFSKSYDVSKLEQDKDLIRNFYQERGYFTSRTLEHDATIRDVGGGKFRI
                     PLFYPNKPGKRADIRIPVEEGRQYKLNKISFAGVKFFRTPESLMRPLFDMDQGDIFST
                     GKLRKGFENLRKLYGEFGYIDFVAEPEFLPLPGTDKIDLTLNFDEGKQFFVRRIDFTG
                     NSTTRDKVIRRELLIDEGDMFNTRLWELSILRLNQLGYFEVLKEEEAAKIERDTKSST
                     VDITLQVKERGKNSVQLSGGVSGIAGSFVSFGYSTNNFLGLGETLSVSSQLGDRIRDL
                     TFGFTEPYFLDRPIQTGFTVYTQRFNFDQGREVSLLSGRDLSPIFNELGVDNLLNYVS
                     NGMGFTVFASYPLKRSFARVGITYGFDSSKYTTLTPAANSYFQYINFQGLEGANSLNG
                     IKTSSIIPSYNYNSVNHPITPTGGKSLFISTRIAGSVLGGNVNLFEPTIDAKYFRGGF
                     KKGHVIGFHFLGRAVMGYGGKVAPPFNRVFMGGEQDVRGFEIWGISPLAYVPSAAEIT
                     VRNNDGSARQQKVIVDGAERLVDVKQTIPIYQMIMPGGDTQLVGNAEYRIPIVGPVTL
                     AFFFDAGMNKIIRPNQLRMNPSRITELNSLFPQAAFNERAVIAPGTQNLRTSTGIELQ
                     IMMPVVNAPFRLYWAYNPTIAETILQPPIVADRSYFPNQTTFLNSIAEFGRPYPFIEK
                     RKTFRFTISRTF"
BASE COUNT         2322 a         3947 c         3957 g         2637 t
ORIGIN      
        1 aggagcgtcc ggtattcacc tggcgcacgg tggaacaggt ggagtttggc cccgacgtgc
       61 ccctggtatt ggtgcgcgtt caaaacggag ccctggtgtc cgcgcccgaa cgcgccgtgc
      121 ggaagcatgc agcgcggtcc atccgcccgc acatcggttt tggctcggac gagatcgttt
      181 cgagagcgga tggcttactt cgcaagttcg acgtcaatac aacggacgcg ggcttcacgc
      241 gcaccccgta ctatttcgcc aagctgacgc tggaggagga cccgaggctg actgcggcga
      301 tggctgccct gctgatggcc accgctttta tagaggcgcc ttcctcgacg ggcttctcgt
      361 tgtgcaccgg actcagcacg gctggagccg gtctatcgat cgcgacggtc acctggattg
      421 ggctggagcc ggtgcgcggg tgcgagcccc tgctgaactt gcagttgctt ttcaatttgg
      481 cgggcctgtt tttgtcgcca ttcactttcg ctattccagg ctccatcgct gtttcgcacg
      541 gaggtttttc atgagcgctc gcaccgatat tccttcgctc cagcgcatac gcttcttcag
      601 cggccagcgc ctcaccgcgg aggacatgat ggccctgcaa aaggcgaatc gcgaattgcg
      661 atggctgcac aaccggagcc ttcacggctg gggaatcggg acgggattcg gcgtcaccgg
      721 cgcacgcggc gccagttccg tcaccatcga acccggctac gccatcgatt gcatgggccg
      781 cgagatcatt ctcaccgaac cggccgtgat gacgattcca gccgtcgccg gcgtgctctc
      841 tcccgaaaac gaattatcgc cggccgtgta ttatctgacg gcgtcgtata aagacgacac
      901 cagccagaac gttgctgaaa aacgtccggg tgtctgtgtg cccggcggca ccgtgcggct
      961 gacggaagaa ccggtgctcg cctggcgtca gccggacaaa atcacggagg ggatggacat
     1021 catcctggcg caggccagca tcctgaactg ccagcttgcc gccgatcttt ccctggctgt
     1081 acgccgctcg gctcggccga gccagcagcc ctatatcgcc gccggacaga ctccgcccgg
     1141 gaacacccaa tggtcggcca tcgttgcggg tggaaacgtc gtaggggttt cgacaacggt
     1201 cagcaccagc ggcgcccgct tccagtctac gccgcagtac ttcgcgcatg tcgtcggcag
     1261 ccgttctgtg agtacgcctt cgtttgttct tgtggtgaat gtactgcccg ttgtgaagga
     1321 ccccgcgccg gaccagtttg tttgcgaggc atacgttgtg tttcccggca gccccacggg
     1381 aggcggtggg agcactcccg cggcagtcga agcgattgtg gaaacgctcc agtggtacgt
     1441 cgtctggatg ggagtggagg gctgagaagt gatcgaggag gcgggatacc tctacgttaa
     1501 tctcgggaac gagtggcccg gtgtgcaact gacaccggca ctcgaaaacg ccggaggcct
     1561 tttgcgcctg cggcccgccg ggagtggatt tgaaaccggc ggagcgtttc tcgccggtcc
     1621 cttccaggta tcggaccagc cggtatcgtg gttccgcgtc gaggcgtttc ttggagagcc
     1681 tgcggggtcg cccgccgcgc cggtgcatgt gcgctttttc acattctcct cctccgggcc
     1741 gcccgcaccc tggaatccgg ccagtgagtc tccgttcagc gatccgggtt ggagtgccgc
     1801 tcccggcaat gcgctcggtt ttcatgcgaa cgttccaccg gggcagaggt tctttctcgg
     1861 cggtgtgctt cgcggagacg gcaacgcatc cccgcagatc gaacagatcc gcatctttca
     1921 cggacgcgac acgtatgcgc gttttctgcc gcccatctat cgcgaggacg ccgccgcgcg
     1981 cgattttctc gaacggtttc tctcgatcgg gcaatcggta ttcggcggca tcgaggaccg
     2041 catccgcgat ctgcctctgc tgttcgatcc gtatgcgtcg ccgcacggcg acccgccttc
     2101 gtggctcacg tggctttccg gctggctcac gttcgtgctc gatgagcact ggacggatgc
     2161 gcaagcccgc gagtacctcg cgcaggcgtt tcaactgtac gggctccgcg ggacgttgga
     2221 aggtttgcgg cggtatctca aaatctacgc gggcgtgaac gcgcacatcg aagaagcggc
     2281 gcagcagacg tacatctggt cgctgggaca ggcgggcgcg cttggattct ccacgatgct
     2341 tgcgcccggg ccattgcagg gcgccgtgct cgacgcctcg gcaacgctgg atcaatcgca
     2401 cctgacgaca ggcgccgagt tcggcgccgc gttattcgag gacgtcgcac accgtttctg
     2461 cgtgcgagtt tattgcggcg aactgacgcg tcccggcttg ctcgaaacgg tacgggccgt
     2521 gatcgatcgc gaaaaacccg cgcataccga gtacgagctt tgtgtgatcg ggccgcaaat
     2581 gcgcgtggga atacaggcgc gcgtcggtat cgattcgatt gttgccgcgg ggccgcgtgg
     2641 cgccgccacc ggaaagccgc tgggcgatgg cgtgctcgcc gcgtctccgt cgcccgaaga
     2701 cggcgatcaa acgccgggag tgcccgttca gcaactctgc ggcgaataga gacaggaaag
     2761 gaaaacgatt atggcaactg cacttctcac ggattgctcc caaacgaaaa tgccaggact
     2821 ggagcgtgtc cgctacttcc cccgccagct tctgagtgcc gacgacatgg tggccgacca
     2881 ggagtatttc cgtaccaagc ttcggcggca caatcgcttc ctgcacggat ggggcacggt
     2941 gtgcggtctg gaggttgcgc ccgcgcccgc cgaaggcagg ccgtggcagg tgcagatcgc
     3001 ctccggctat gctctcggtc cttacggcga tgaaatctat gttcgcgatg ccgtgctgct
     3061 ggacctggcc gaatgcggtc cgggcgcgga gaccgacccc tgcgcccccg gattcattct
     3121 gcgcggtagc gtgtctcgca cgggagccac gttgtacgtt gcgctgcgct acgaggaatg
     3181 cctctcgcgg ccggttcgcg tgctgcccgg cggctgcggc tgcgaagaca cctcctgcga
     3241 gacgtcgcgc atccgtgaca gctttgtcct ggaatgcctg acggagctgc cgccttcgca
     3301 cactacgccg cccggtcctt cgttatgcga tctgcttcgg ggccgtgcgc tggcgcaatg
     3361 ccctccccgc cccacggatc catgggttgt gctggcgcag gtgcgtctgc cggcggatcc
     3421 atccactacg atcccggcca accagatcga caacttcatt cgccgccagg tattcagtac
     3481 cgccgctatt caggaccagg tgattcgctg ctgctgcgga ccgcaggagc gccgcccggc
     3541 gcgcgtcacc agcatcaacc cgccaccgaa ctcgcgattc accagcggaa ccgcggtgcc
     3601 cgccgccgtg gtgatcacgt tcagcaagaa ccttcaggcg cagaccgtga ataccgatac
     3661 ggtgcaggtg ctgcgtttcc tgcccaatca accggcggtg tttgtgtccg gacaggtgac
     3721 gtacgacgac gggaaccgtg tggcccgatt cacgcccgcg gctccgttca cgcaaaacgg
     3781 aatctaccag gtgaacgtgg caggcagcgg gcccaacccg attgtggatg ccgacaacct
     3841 tgctctggat ggcaatgacg acggattgcc ggggggcaac ttcgtaagcc agttcaccat
     3901 tgaagctcct tcgacgacgc ccacacccac accgacaccc acgcccggca gaccgctgag
     3961 cgtgaaggtt gcaaccgtgg gggacatccc caaggtcatc cccaacaacg agccgggctc
     4021 tgtcgccggt ctggcgctgc tgttcacggg agggactccg agacaggagg tagaggcgaa
     4081 tctcatggtg ttcctgaacg tcaatgtggc caccagcacc ggacctgttg tactgctgaa
     4141 ccagatcacg ggggagagta tgaacgcgca gatcggtcag aacaactatg ctttcccgaa
     4201 tgtgaagttc acgatgcccg ccacgggcgg tgagcagcca ttcgagatca acaaaatggc
     4261 cgtgatcatt cctcgtgatg gccccaatat ccaggaggtc acggcattag tatccgttac
     4321 cagcgctacg cccatcaccg tcgaaaatcc acaggtgacg gttgcttttg tttcgtaacg
     4381 caatcgaggt gatgcggtgg cgaacggtaa tgtacaacca aaccggcaat tgcgtgcccc
     4441 cgcccacgcg gatttccgtg cgggccggta tctccgggcg gccgacctcc agttggaaca
     4501 ggcctaccgg ctggaacgcg cacggcggca caatcgcttg ctgcacgaat ggggcattgt
     4561 ctgcgggctc tgggtggcag cggcggcaga ccccgcacgg ccgtgggcac tgtatgtgtg
     4621 tcccgggttc gccatcgggc cgtatggcga cgagatcgaa gtgcgcgagc gaacgctggt
     4681 gaacgtgagg gatttcctgt gggccaagcc acccgaaggc aagctggagc ttcgccgggc
     4741 tttcgtggcc atccgttacc gcgaacggcc gcggcgtctg gcagccctcc ccgccacgcc
     4801 ttgtgcctgc gatcatccgg aatacgccgc atcgcgcgtg gccgatggct tcgaactcgc
     4861 cgctctttgg acaccgccca aggtgcgcgt tccgcaggga atttgcgaac ggacggcccg
     4921 cctgtgcccg gaatgtcccg aatccgggtg gattgtcctg gcgcgcgttc ttctccccgc
     4981 cgctccttcg caactcatta cacaggccga tatcgacaac ggcgtccgga gtgcgctgtc
     5041 caactccacc tccggaagtt tcatcgcaga gagagagtaa cgactatggc aagattcggt
     5101 gaggactcgc aagcgccaac caggctcgct acacccgagc ggaacgccta ttttttcggc
     5161 aagctgctgg gcgtgtccga tttcgatctc gagcaggatt acttcaattc caaacgttgg
     5221 ctcatgaacc ggctgctcgc cgggtccggc gtagcgtgcg ggcttggtgt ggctccggtg
     5281 gtagacggaa cccggctggt gatcgatccc ggagtggcaa ttgacggatg gggccgcgag
     5341 atcatcgtac cggaacagag cttcccattc gatccgcgcg tgctgagaga tgcggaaggc
     5401 aacacgagcg gaagcatcga gggcgagggc accgttgtga tttcgctctg ctaccatgag
     5461 tgcggcgccg aacccgcccc ggtgctcgcg cccggctgcg atccggaagg agactgcgcg
     5521 tcgtcggtga cgcgggagaa ataccgcatt gtcgtgaccg agggcgtagc ggttccaagc
     5581 ggtttggatt gtcgcttcgc ctccctgttc actccggcgg aggattcgga ccagcttccc
     5641 aatccgcttc ccgcgctggc ggagcacatc acctccgcct gcacggaggc gagcggaggc
     5701 gggtgcatcc tgctcgccca ggtcagcctg cctgccgaag ggggcattac tccggagatg
     5761 atcgatgtcc gtgtgcggcc agtcgtgctg agccatggga ttctgctgga actgatcttc
     5821 tgccttgcgc ggcgtgttca ggagcttgca gcgggtccgg tgccgacccc gacgccaaca
     5881 ccaacaccgg aacccactcc tacaccaacg cccgagccaa caccaacacc agaaccaacg
     5941 ccaactccgg caccggaacc aacacccaga ccgactgctg aaccagtgcc cacgcgcaca
     6001 ccggcgcctt cccccagacc cacactggga cctgtgccta ccgttttgcc tacacgtacg
     6061 ccggtgcctg tgcccacagt tctgcccaca cgcacgccaa cgccgcgtcc cgcgggaaag
     6121 cgcgtgccgc gtcccacacg gcgtccgtga tgagcgctga agtctcgatc attgtcacca
     6181 gccgggatga ggatcccgcc gtcttgcaag cgactctgga cggcctccgc acgacaacgc
     6241 gcggagtctc cacagagatc atcgtcatcg acgattgcag ccgcgctccg gttgtctgcg
     6301 aagggtctgg cgtatctctg gtgcgaaaca gccggcccat aggggtgtgc ggctcgcggc
     6361 ggctcggcgc cggattggcc ggcggtaaag tgctggcctg ggtggatgcc cacatgtcgt
     6421 tcggcgaaca ctggctggag cagatgctgg tgcacgccgc ctccggtgca ctgctctgtt
     6481 cacctttctg ggattacgat cttcagacct gtctttgctg gggcgcggac ttcatgtgga
     6541 acggcacgcg gaattacatg gagcaaaaag ttccgggctt cggattgctg caccgcgtcg
     6601 cacccgttcg ggagcttgcg cccgaggtcc ccatggtgat tggcgcctgc tatatgatgc
     6661 gccgtgacag ctatgagcga atgggcggct tctcgccgtt gttccgcatc tggggcatcg
     6721 atgagcagga cctctcggcg cgcgcgtgga tctccggcgc gggcgtccgc tgcgtgacgt
     6781 ccgcaaaggt gggacacctt tcccggacgg catttcccta tccggtctcc ttcgaccaca
     6841 tcgaattcaa tcagatcgtg atgctgagga cgctcttcga gccacagaca gtccgcaagc
     6901 tggagcgctg gttcgaaccg attccgccgc aagtgcgttc gtggcttagc aacactggtc
     6961 tggcttcgtg gcgcaacacc gtacaaacct gccgccgcct ttcggaccgg caatttttct
     7021 tacgcttcct gcccgggctg gcggacgaac tgggtctccg tgcagcataa gtacacggta
     7081 cagcgggacc gctccctcac ggtcgcggct cagaaacgga gccgcgcgcg ccagcaagcg
     7141 gcatcggatg gtcggtgatg caaaccagta ggcaaaatag tacactggag gaacgaatca
     7201 ggacaatcca tgaatcagca gatccaggct atcgcggaac tcgaccgggt gattcacgaa
     7261 cccggccggc tcatgattgt cgcccttctg ttcgctgtcg aaaaggcgga ctttctttat
     7321 ctgcagcacg agacggccat gaataaaggc acgctttcca gccatatttc ccggctggag
     7381 gaggccggct acgtcgaggt ggtgaaaacc tatcgcggaa aggtgccgca gacgctcctg
     7441 cgcctgaccg gggcgggccg caaagcgttt gaacagtacc ggcgaaagct caaagaagca
     7501 ctgtccgtgc agggccgcca acccgatcgg ccgcggcatc cgcggccggt ccgtatcgcc
     7561 gaatcgcacg cgtgaataac ggttgcggcg attacgctat gaactgctcg gcgtcggggt
     7621 tgcccggtcc gaagtgtttc agcatcacca ggttttccgt gctgctgcgg ttggtgaccg
     7681 tcacccccac ctccgctgtc cgggccgaaa caaacaattc gtcttttgtc atctctccgt
     7741 agcggatcag actgggcgtc tccacgtcct gtttgccggc gctaccccat ccctgcacca
     7801 cgatcaggcc ataagccgcc gcatcctgaa tcgtgaccga gcgcccaggc agcacggtga
     7861 gctccttcgc cgaataattc ggcgttgagt aaacgatcca cttctccgag taaccggcgt
     7921 cgagcatttc cgcttccggc ttcaccggcg tgggatggaa caggcggtgc ttagcaaact
     7981 ccggatcgac gttcgcttcc cagtcgagca tatccagaat gtagtcgagg tcgtgatgat
     8041 gctccggtgg gacatctttt accagcaggt cccacggcac ggcgcgtcct tccaccatcg
     8101 actggaacat gccgaacaca tcggaattca cctgcggctc gtaggtcacc agagaaccgg
     8161 gcgcgtgcag aatgcccgcg tcgatctgcc atccggtgcc gggctgcaac cggtaggcct
     8221 ttgagtggta gagaatgccg ttatcgcctt cgttccaacg ctccaggcag cggcggatgt
     8281 cgtcgcgcgt ggtgcccggc tccagtccca taaacgtgta agggaagttg ttgtccttga
     8341 aattatactg aggcgggaaa taataggctt cgggtttgcc gttccggccc acctttttcg
     8401 catgctcttc cttctggtgc atgtggtggg gaatggggcc gagattgtcg aagaatttgc
     8461 agagcagatt ccagccgccg tatttgctca tcacgccggc cccgaggaat tcgtctccgc
     8521 gcgtctcgat ggcttctttc aggagcacgc gctttccgtt cagggcgatg tagctgagac
     8581 cttcatcatc cggtgtgccg ggaccgttgt cggcccgggt ggtagaggag aaccatcgtt
     8641 cgtcgatgcc gccgcggtga gcgccgagcg cgtagaggtc gcggggatcg agcttcaatc
     8701 tccctccggg catcaggaac gaccggggga cccaacacgg agccagccgg acgatgccat
     8761 ccgccgcaca aagggcatcc tttatcaatg attcattgga catgatcgcg atccattgta
     8821 ctgagggcgc gaggcgggtt tcaatccggt ggcggcggcg agcgaaattg gccgctcccg
     8881 ccgctgctat gggcttcggc cgcccttact tcggagcgcc gggtttcggc ttggcggccg
     8941 cgggaggcgt gggagggggc gtggtggtgg cgggcgccgg agatgcggag ggagtggtag
     9001 ccgcccccgg agcgttcttg tcgtacaggt cgatgatgtc cttggtgatt tcgatgctgt
     9061 tggcggcgta aagcaccggc gtttgcggcg aactgatgtc aagaatgagc gtgtaaccgt
     9121 gatcgctcgc atacttgttg atgactgcca tcatctttcc gccaagttcc tgcaacacct
     9181 tctgctgttc ctggtcccat tccatctggg cgtcttccag gtcgcggttg aacgccttgg
     9241 tcttctggtc gatatcgcgc atcaggccct gccgcgcggt ttccgccatc gtgttgctgc
     9301 ccttactgag cttctcttgc aggctcttga tttcctcctg ctgcccctga agttgcttcg
     9361 ctttgggagc gcgcttggct tcgatttccg atgccgcttt ctggccttct ttggtgccaa
     9421 cgatggcggc ctgtatgttg ataatgccga ccttggtggg cgtgttggcc tgcgcgtagg
     9481 cgaaggcggc ggcgccgagg actagcgcag gtagcacaaa ttgctttctg ttcactctct
     9541 taactcctaa ctgtcgtaga tggccgcccc gctagccttc cggcccggca acatggagcg
     9601 tttgcaacag cgttgcgtgg actgacttca tcactattta gaacgtccgg ctgattgtga
     9661 accggaaggt ctttctcttc tcaataaacg gatagggccg tccgaactcg gcgattgaat
     9721 tcaaaaacgt cgtctggttc ggaaaatacg aacgatccgc gacgatcgga ggttggagaa
     9781 tcgtctcggc gatcgtagga ttatacgccc agtataaccg gaaaggcgcg ttcacaacgg
     9841 gcatcatgat ctgcaactcg ataccggtag aggtacggag attctgggtc cccggagcga
     9901 tcacggcacg ctcgttaaac gccgcttgcg gaaagaggct gttcaattcc gtaatccggc
     9961 tgggattcat ccggagctgg ttgggccgga taatcttgtt catgccggca tcgaagaaga
    10021 acgctagcgt cacgggtccg acgatcggaa tgcggtactc cgcgttgccc accaactgcg
    10081 tgtcgccgcc tggcatgatc atctggtaaa tcgggatcgt ttgcttcacg tcgactaaac
    10141 gttcggctcc gtcgacaatc actttttgct ggcgggccga gccgtcattg ttgcgcacgg
    10201 tgatctccgc cgcgctggga acgtaggcga gggggctgat cccccaaatc tcgaatccgc
    10261 gaacgtcctg ctcgcctccc ataaataccc ggttgaaggg cggggccacc ttcccgccgt
    10321 accccatcac cgcgcgtccc aggaagtgga acccgatcac atggcccttc ttgaagccgc
    10381 cccggaagta cttggcatcg atagtcggct caaacaggtt tacatttccg cccagcacgc
    10441 tgccggcgat cctggtggaa ataaagaggg actttcctcc cgtcggcgtg atcggatgat
    10501 tgacggaatt gtagttgtac gaagggatga tgctgctcgt tttgatcccg ttcaggctgt
    10561 tggcgccctc caggccctgg aagttgatgt actggaaata gctgttggcc gccggggtca
    10621 gggtggtgta cttggacgag tcgaaaccgt aggtgattcc cacccgggcg aaacttcgct
    10681 tcaacggata gctggcgaac acggtgaagc ccatgccgtt ggagacgtag ttcagcaggt
    10741 tgtccacgcc cagttcgttg aagatcgggc tcaggtcgcg tccggaaagc agagatacct
    10801 cgcggccctg gtcgaaattg aagcgctgcg tataaaccgt gaatccggtc tggatcgggc
    10861 ggtcgaggaa gtagggctcc gtgaagccga acgtcaggtc gcggatacgg tcgcccaact
    10921 gcgaggagac gctcagcgtt tcgcccaatc ccaggaagtt gttcgtcgaa taaccgaagc
    10981 tgacaaaact gccggcgatg cccgaaacgc cgccgctgag ctgcacggag ttcttcccgc
    11041 gctccttcac ctgcagcgta atatcgaccg tgctgctctt ggtgtcgcgc tcgatcttgg
    11101 ccgcttcctc ttccttcagt acttcgaagt agccgagttg gttcagacgc aggatgctca
    11161 actcccaaag acgggtgttg aacatatcgc cttcgtcgat cagcaactca cggcggatga
    11221 ctttgtcgcg cgtcgtactg tttccggtga agtcaatgcg gcggacgaag aactgcttgc
    11281 cttcgtcgaa gttcagggtg aggtcgatct tgtccgttcc cggcagcgga aggaattcgg
    11341 gttcggcgac gaagtcgata tacccgaact caccatacag cttgcgcaga ttttcaaagc
    11401 ccttccggag cttcccggtg gaaaaaatat cgccctggtc catgtcgaac aatgggcgca
    11461 tcaggctttc cggcgtccgg aagaacttca caccggcaaa actgattttg ttcagcttgt
    11521 attggcggcc ttcctcaacg ggaatacgga tatcggcgcg cttgccgggc ttgttcgggt
    11581 agaacagagg aatcctaaac tttccgccgc cgacgtcgcg gatggtggca tcgtgctcta
    11641 gtgtgcgcga cgtgaagtag ccgcgttcct ggtagaaatt ccgaatcaga tccttatcct
    11701 gttcgagctt gctcacgtca taggactttg agaacaggcc ctcgaacaga atggagtggg
    11761 gaatcccgat cggccgcagg ttcttcatcg accgtattac ggcgcgatcg ccaaaaaccg
    11821 tgttgccttc tatgtcgatg tcgccgactt taaccttcgg gccttctgtc accttgaacg
    11881 tgatctcgag cgaggagggc gggatctgcc gcacctgcgg ttccacgacc gcgaactgcc
    11941 gcccgcgttc ggccaggaat tccttcaaca cgaccgtcgc gcgctggacg cggttcggat
    12001 cgtactgcga ctcgacgctg agaccgacac gccgctcttt gaagcggtcc aggatctctg
    12061 ataccgttac agacttcgcc ccttcatact tgatggaccg cactactctg cgttcggtca
    12121 gcaggaagcg cacgatccat ccggcgttcc ccggttcgcg ctccagccgg atatcgtcga
    12181 accttcccga gttccacagg gccatgaagt cgcgatgcaa cgcctcttcg tcataccggt
    12241 cgcccctttt tgtgaaaatc atcgcgcgaa gggtgtcctg gggtacgcgc cgcgacccgc
    12301 ggaactcaat cgcctcgatg atatccggtg tcttttcggt cttgggcggc tcggctgccg
    12361 gcttaggcgc ttcaaacggc gtttggccgg gcttgggttg cgccggcttg ggcggctgcg
    12421 gcttgggagg ttccgcctgg ggaaccgtct cgaaagggtt gctttgctgt tgcggcggct
    12481 ggcttgccgg agccgtactc tgctgcggag atgcttgggg actggcggcg cttgccaggc
    12541 aggacagaca tactgcggct atgctcaggc gtttcggcct gcgaaaactc atgaaacgcg
    12601 aattcctttc ctccctgcgc ctatccgcag cagacgtcta ccgcagcaag gtggtttcaa
    12661 cacactgatg catccgggat gatggcgcaa gcttaagctc cattttatca aacgggggtt
    12721 ccgccgcccc cgctggaagc cggctcgacg cgattgtggc cgggcggttt ccgtccttcc
    12781 gcggcggccc gctcgagaga gtaaaccgcg gccggatagc ctgccagaaa ccctccgtag
    12841 atgccgtgcg cgatcaacat gga
//
LOCUS       DASTMK010000060        13935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_195113, whole genome shotgun sequence.
ACCESSION   DASTMK010000060 DASTMK010000000
VERSION     DASTMK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13935
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195113"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..487)
                     /locus_tag="VFQ79_02460"
     CDS             complement(254..487)
                     /locus_tag="VFQ79_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138538.1"
                     /translation="MQAIELVGDVDEQHRLHAQVPKEIPAGRVRVIVFLPGEDEAGDL
                     WAGGVASEWSAELSDAREDIYTLEDGQPVNAGR"
     gene            630..1016
                     /locus_tag="VFQ79_02465"
     CDS             630..1016
                     /locus_tag="VFQ79_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0138539.1"
                     /translation="MEINKDLIAASSTPIVLAILAEGDSYGYAILQRVRELSGGRMEW
                     TDAMLYPVLHRLERLGHVKARWEVAGSGRRRKYYRITSQGRAQLAEERRQWQAVDSTL
                     RGIWRTISLAVPDSRAFSAASVPGEA"
     gene            1016..2386
                     /locus_tag="VFQ79_02470"
     CDS             1016..2386
                     /locus_tag="VFQ79_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEU0138540.1"
                     /translation="MSTPGDAVSLEEQIDQWRSYLRRRQAIHSVDVAELEDHLREQVA
                     GLIAAGLSTDEAFLVAVKRMGNLDALSREFAREHSNRLWRQLVLVPSDSGELQAPGRM
                     DAIVAFCLAVAAALAVKVPEFFGRDLDKYAGFYMRNASFFVLPFLTGYFVWKRRLDFK
                     TLRWLAVAFIVASVFANIYPFTANGHTEVLAALHLPIALWLMVGIAYAGGRWSQVAGR
                     MDFIRFTGELFIYYVLIALGGLVLIGFIVTIFRAIGINAAPFIESWLVPCGAAGAVLV
                     ASWLVEAKQSVIENMAPVLTRLFTPMFAAVLIMFLGTLLWTGRGIDIARDVLIAFDLL
                     LVVVIGLLLYSASARDPRLPPDAFDVLQVVLVVSALLADAVALSAIAARISEFGFSPN
                     RVAALGENVILLVNLAWSAVLYIRFLRGRGSFASLERWQTSYVPVYAVWAAIVVIVFP
                     PVFRYA"
     gene            2447..3850
                     /locus_tag="VFQ79_02475"
     CDS             2447..3850
                     /locus_tag="VFQ79_02475"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0138541.1"
                     /translation="MFADIYGTVTARVVLFTSSLLALVSIGASTATAQPGGSLTPRLD
                     SIAGAGVRENRAVGIAAAVVKGQDTLLLEAYGKADVEGNVPMTVDTVIPIGSTTKQFT
                     AAAILQLRDQGKLSLDDDITKWLPDFDTRGNKVTLRHLLSHTSGISDLAAMPELRAMR
                     LIRNPTATRDDVYKVVHRHPFLFPTGTMEIYSNTNFWLLGLVIEKASGMTYEDYVEKK
                     IFEPLGMTRSTYCNNSENVPRRTYGYFMKNGVSHRVPDIVHTGTYAAGALCSTAKDMI
                     AWLQALHGGKVLPPRSYAEMIAPSKLNDGTPLRYSLGTEIGEDRLGFRYIGHGGGGFG
                     FSSQVRWYRDGQLSIVVLTNSEPDNITVVTEDLAAAVLPPPRRAGPFKGDASLLSGKY
                     KGPGRGTEMVIDVTHTPEGMAFSFDGAAAVPLPWVESWTFRRGSSLLTFRRSTTTGPA
                     TELRFDTAGDYFILKRQ"
     gene            complement(3862..4794)
                     /locus_tag="VFQ79_02480"
     CDS             complement(3862..4794)
                     /locus_tag="VFQ79_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0138542.1"
                     /translation="MELFTLKVFQAVATEKSFSRAAEKMLRTQPAISLAVQRLETELN
                     EKLIDRSAKELLLTDAGRIVLEYARRFENLERDLENSLAELRDHSAGRLVIGANESSS
                     LYLMQHIVEYRRLYPKVKVQVRRSLSSRIPAQLIDGDLELGVISFDPEDDRLVSSVIF
                     TDHLAFVVSPHHRFAGRDTISISELGMETFIAHNVLSPYRERVLREFQRHKVPLNMDV
                     EMPTIETIRRMVQRNEGVAFLPKMCVEREIQQGTLWEVNVREINMERKIRLLYPARRA
                     LSHAARAFLEVVQGAQGQRSEQPSAIGHPLPVSK"
     gene            5028..6197
                     /locus_tag="VFQ79_02485"
     CDS             5028..6197
                     /locus_tag="VFQ79_02485"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU0138543.1"
                     /translation="MNNRVYIFDTTLRDGEQSPGCSMNQQEKLKLAHKLVELGVDILE
                     AGFPIASEGDFEAVDAVSREFPWVQVAALARACTLDIERAARALEHAKRPRIHTFIAT
                     SEIHLKYKLRKTQQQVLDEAVAAVELARRYADDVEFSAEDATRTDMDFLEKISRAVVA
                     AGARTVNLPDTVGFSVPDEYGELIGRMVRSLGDSAILSVHCHDDLGLAVANSLAAVKA
                     GARQIECTINGIGERAGNCSLEEVVMAINTRRDRLPYHTGIVTEHLFPASQLLTEMIS
                     FGPQPNKAIVGENAFAHEAGIHQDGYLKERSTYEIIDPKSVGVPEGRLVLGKHSGRHA
                     LNKRCQDLGFELTRDELDLVYKQFIALADRKKGVRNEEIAVLVRQEIARVATAAD"
     gene            6221..7303
                     /gene="leuB"
                     /locus_tag="VFQ79_02490"
     CDS             6221..7303
                     /gene="leuB"
                     /locus_tag="VFQ79_02490"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU0138544.1"
                     /translation="MNLKVLILPGDGIGVEVTREAVRVLEHVSRKYKHTLEISEALLG
                     GAAIHQSGTPLPDKTVELALKADATLLGAVGLPEFDNVPPEKRPEKGLLGIRKALGVF
                     ANLRPVRTYSSLLESSPLKNHLIEGTDMIIVRELTGGIYYGTPRGISGEGAETTAVNT
                     MVYTRAEIERVARMAFRLARGRRKKLTSVDKSNVLENSQLWRKVVTEVAGEFNDVALD
                     HLLVDNCAMQLVLNPRRFDVVLTENMFGDILSDEGAVLAGSIGMLPSASIGEQRESGA
                     WVGLYEPVHGSAPDIAGQNKANPLGAIGSVAAMLEYSFGLKQEAEAVNRAIETVLGQG
                     RVTADLKPKGAPATTSEVGEAVCSAI"
     gene            7338..8744
                     /gene="leuC"
                     /locus_tag="VFQ79_02495"
     CDS             7338..8744
                     /gene="leuC"
                     /locus_tag="VFQ79_02495"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU0138545.1"
                     /translation="MRKPRTVIEKVWDSHVVTEQPGAPALLYIDLHLVHEVTSPQAFT
                     GLRARGLKVRRPDLTLATTDHSIPTTPRTFPIVDSVAAAQVAQLESNCAEFGIPCYGF
                     HSEKQGIVHVIGPEQGLTQPGMTMVCGDSHTATHGAFGALAFGIGTSEVEHVLATQCL
                     LQRRSKTFQVRVDGALRPGVTAKDIILALIARIGVGGGTGCVFEYTGSAIRALSMEER
                     MTICNMSIEGGARAGMVAPDDTTYEYMCGRPFAPKGKEWEAALARWRQLPTDEGATYD
                     LEVTLDAAELEPMITFGTNPGMGIPITGPVPDPSLLNDVQERDSLAKALRYMDLQPGR
                     PLLGHPVNVVFIGSCTNSRISDLRAAAGVLKGRKVNPNVRVMVVPGSQEIKRQAESEG
                     LDRVFRESGADWREAGCSMCIAMNGDQLQPGEYSVSTSNRNFEGRQGKGGRTFLASPL
                     TAAATAITGVVTDVRTLL"
     gene            8741..9352
                     /gene="leuD"
                     /locus_tag="VFQ79_02500"
     CDS             8741..9352
                     /gene="leuD"
                     /locus_tag="VFQ79_02500"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU0138546.1"
                     /translation="MKKFTPFESRLVPLPVDNVDTDQIIPARFLKTISKEGLGDQLFY
                     DWRYDAAGAPKPDFILNQPQGKQGQILLAGDNFGCGSSREHAPWALTQFGFRAVISTS
                     FADIFKQNSLKNSLLPIVVPRDIHAALFELVSSDPAATIRVDLAAQKIVLPDGREVEF
                     PVDAFAKHCLLEGVDELGFILQQEPAIVRYEAQRVSSLDTRAG"
     gene            9398..9703
                     /locus_tag="VFQ79_02505"
     CDS             9398..9703
                     /locus_tag="VFQ79_02505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU0138547.1"
                     /translation="MIIVHVFAHVKPEHVRAFREATAENARNSVQEPGVARFDVIQEK
                     DNPTRFMLAEVYRTPEARAQHKETRHYQVWRDTVAPMMAEPRTSIEYSNVFPSDQDW"
     gene            9708..10862
                     /locus_tag="VFQ79_02510"
     CDS             9708..10862
                     /locus_tag="VFQ79_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU0138548.1"
                     /translation="MSFEFATASRIVFGRGAAREIMPAARRMGARTLVVTGRHPERAG
                     WLLTSLKAAGLEPAVFPVDGEPTIDSVRNGVELARERACDVIISLGGGSAIDAGKGIA
                     ALLANGGDPLDYLEVVGKGQALEKPSAPFIAAPTTAGTGSEVTRNAVLASPPDKVKAS
                     LRSPFMLPELAVVDPELTVDVPPDVTTFTGLDALTQVIEPYVSARRNAMTDLFCLEGM
                     RRVSRSLGRAYDDGASIEAREDMAFASLMGGLSLANAGLGVVHGFAAPIGGMFDAPHG
                     AVCAALLPHGITANIRALSDRAEDREVLDRYQTVARILTGDAGASAEDGAAWVRELCR
                     RLRIPGLAAYGVDAAAIPVLVEKASGASSMKANPLVLTREELTDTLERAL"
     gene            complement(10859..11500)
                     /locus_tag="VFQ79_02515"
     CDS             complement(10859..11500)
                     /locus_tag="VFQ79_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138549.1"
                     /translation="MFKLLLSSFLFFTLAAAAQDRPGLFFREDWKETPPAKPVTLDHL
                     ANSNLTFTLYGPGKEGIKKSNHDKPADDPFYIWTGECVGNCAIALKHKTSYVDLRGQS
                     KLKWRSKQAGFRGARIILKLANGTWLVSDAYDGPSLDWREREFNIQDIRWRRLDIDKI
                     IEGAWVDNPDLSRVDEIGWTDLMTGGGSISCTRVDWIEIYGKPVPRGQSPSGN"
     gene            complement(11535..11795)
                     /locus_tag="VFQ79_02520"
     CDS             complement(11535..11795)
                     /locus_tag="VFQ79_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU0138550.1"
                     /translation="MPVQPLDTPAGSTPWTPTERHLVRPLDEKKIGGVCAGVGHYLGV
                     DVTLVRIVWLIVAIFTGAGFIVYLVFWAAMPGQRGTSAAQNA"
     gene            complement(11967..13385)
                     /locus_tag="VFQ79_02525"
     CDS             complement(11967..13385)
                     /locus_tag="VFQ79_02525"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEU0138551.1"
                     /translation="MRSLVWLLTVCCATVATLKSQRAPLQVHRPEIADCDDVAFGPDE
                     DIYLACHSPYDRLPIDVRGAKAADEMDAYVLRLNPRTGKLVFATRFGGRSFDAALRIK
                     VDKNGYAYTTGLTKSPDFPTTKDALQPKLAGGSDAFLVKVAPGGEIVYATLIGGARDD
                     LGNGLDLDERGNVYLGGVTSSSDFPAQGNNRLTTEDDAFVCWFRAVEHAISCEIFGGR
                     KGEKLTGIVLDRAGGIYAVGSTTSPDFPVKDPVQNALAGPSDLFLTRLALPSLEMTSS
                     TFFGGSGEDSGWGIALDMRGNPVMAGITNSVDLPGVSASYQPRNRGKRDAFIASIGMR
                     HGREIRSTYFGGSNDDESGYDGGDIKVDRHGNIWLAGITYSDDLPTRNAGQPRFGSGN
                     GDGFVAAFSADLTKLCFASYFGNKERNLLEGLAISPAGLVAATGVSFSQAPSASHIQI
                     GQTNLHAGANVIVFHAETACSR"
     gene            complement(13440..13778)
                     /locus_tag="VFQ79_02530"
     CDS             complement(13440..13778)
                     /locus_tag="VFQ79_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU0138552.1"
                     /translation="MGVALIVDDPDAPRGTWNHWLLYDIPANIHSLAQGFKPGQLGRD
                     GINDFGKQGYGGPCPPKGHDPHRYFFRLYAMDTDSLGIPDGARRADLDRALQRHTLAQ
                     AEYIGHYQRH"
BASE COUNT         2779 a         4142 c         4237 g         2777 t
ORIGIN      
        1 ttacgaaacg acgatgtagt aactgcgctc agcctggccc aacgcgccga cttgctggca
       61 aaagacctca actcgcggct caaatagccg cctacgctct ctatcgcagt gactaagtaa
      121 ccctattcgg aattacggtg acctatccga aaccgctcgc tgacgcgcgc ggctccgtaa
      181 tcgtttcgga gccgcgaacg taagtgagcg gttggcatgg tggcgagcca agctcgacga
      241 gtggaggaag atttcaccgg cctgcattca cgggctgacc gtcttcgagg gtgtagatgt
      301 cctcacgcgc atctgatagt tccgccgacc actcggacgc aactccaccg gcccaaaggt
      361 caccggcctc gtcctctccc ggcagaaaca cgatcacgcg tacccggcca gcaggaattt
      421 ccttcggcac ctgtgcgtga agccgatgct gctcgtcaac gtcgccgact agctcgatgg
      481 cctgcatgaa ggaacaccgt cgatgattct agcaaagccg ccggctttcc gcctgaaacc
      541 aaggctgcct tgacatgcct aacagcatta tgcataatac tgccatgtat gctcggatcg
      601 attgattagg ccgcgctact cacgaaaata tggaaatcaa caaggacctg atagcagctt
      661 cctccacccc gatcgtcctg gcgattctgg ctgaagggga cagctacggc tacgccatcc
      721 tccagcgcgt gcgggagttg tccggaggtc gcatggagtg gacggacgcc atgctctacc
      781 ccgttctgca ccggctcgag cggctcggcc acgtcaaggc acggtgggag gtcgccggga
      841 gcggccgccg ccgcaagtac taccggatca cttcccaggg ccgggctcag ctcgccgaag
      901 agcgcaggca gtggcaagcg gtggactcga cgctccgggg catctggagg acgatttccc
      961 ttgccgtccc tgacagccgc gcgttctcag ccgcatccgt gccgggggaa gcctgatgtc
     1021 cactccaggt gatgcggtat cgcttgagga gcagatcgac caatggcgca gctatctccg
     1081 ccgccggcag gcgatccact ccgttgatgt agcggaactc gaggaccatc tgcgcgagca
     1141 ggtagcgggt ctcatcgctg cggggctctc gaccgatgaa gcgttccttg tagcggtgaa
     1201 gcgcatgggc aacctcgacg ccctctcccg ggagtttgct cgcgagcact cgaaccgcct
     1261 ctggcggcaa ctcgtgctcg tgccatcgga ttccggggag ttgcaggcgc cgggccggat
     1321 ggacgccatc gtcgccttct gtctggcggt cgccgcagcc ttggccgtca aggttccgga
     1381 gttcttcggg cgggatctgg acaagtacgc aggcttctac atgcgcaatg cgagtttctt
     1441 tgtgctgccg ttcctgaccg gctatttcgt ttggaaacgg cggcttgact tcaagactct
     1501 tcgctggctc gccgtggcct ttattgtggc gagcgttttc gccaacatct atccattcac
     1561 cgcgaacggt cacacggagg tgctggccgc gctgcatctg ccgatcgcgc tttggctgat
     1621 ggttggcatc gcatacgccg gcggccggtg gagccaggtc gcaggccgca tggatttcat
     1681 tcgattcacg ggcgagctgt tcatctacta tgtcctgatc gccctcggcg gcctcgtcct
     1741 catcggattc atcgtgacga tcttccgggc tatcgggatc aacgccgcgc cgtttatcga
     1801 gtcgtggctg gtgccctgcg gagcggcagg cgccgtgctg gtcgcctcct ggctcgtgga
     1861 agcaaagcaa agcgtgatcg aaaacatggc cccggtgctg acgcgcctgt tcacaccgat
     1921 gttcgccgcc gtgctgatca tgttcctggg gacgctgctg tggaccggac gcggcatcga
     1981 catcgcgcgg gatgtgctca tcgcgttcga cctgctcctg gtggtggtca tcggcctcct
     2041 gctctattcc gcatccgcgc gggacccgcg gttgcccccc gatgccttcg atgtcctgca
     2101 agtcgtcctg gtggtgagcg cgctgctggc cgatgcggtc gcgctgtcgg ccatcgccgc
     2161 acggatctcg gaattcggct tcagccccaa ccgtgtggct gccctgggcg aaaacgtgat
     2221 tctcctggtg aatctcgcgt ggtccgccgt gctctacatc cgctttctgc gcggtcgcgg
     2281 atccttcgcc agcctcgaac gctggcagac cagctacgtg cccgtctatg ccgtgtgggc
     2341 ggcgattgtg gtgatcgtct ttccacctgt gttccggtat gcctaggggc tgctgaaaaa
     2401 agatgaaaac cgcttgctcg cgcgcgcggc tcagaaaggt gatcacatgt ttgcagacat
     2461 ttacggaacc gtgaccgcga gggtagttct ttttaccagc tcgctgctcg cgctcgtctc
     2521 gatcggtgcc tctactgcca ccgcgcaacc cggtgggagc ctgacgccgc ggctggactc
     2581 cattgccggc gccggcgttc gcgagaatcg tgccgtcggc atcgcggccg ccgtcgtcaa
     2641 aggacaggac acgctgctcc tcgaggcgta cggcaaggcc gacgtcgagg gtaacgtgcc
     2701 gatgacggtg gacaccgtca ttccaatcgg ctccaccacg aagcagttta ctgccgccgc
     2761 gatcctccag cttcgcgatc aggggaagct cagcctcgac gatgacatca cgaaatggct
     2821 gccggacttc gacacccgcg gcaacaaggt gactctgcgc catctgctca gccatacgtc
     2881 cggtatctcc gatctcgccg cgatgccaga gcttcgcgcg atgcgactga taaggaaccc
     2941 caccgccact cgcgatgacg tctacaaagt cgtccatcgc catccgttcc tgttcccgac
     3001 gggaacgatg gagatctaca gcaacaccaa cttctggctc ctcggcctcg tcatcgagaa
     3061 ggcgagcggg atgacgtacg aagactacgt ggaaaagaag atcttcgagc cgctgggcat
     3121 gacgcgctcg acgtactgta acaactcgga gaacgtgccg cgccgcacct acgggtactt
     3181 catgaagaac ggggtttccc accgcgtgcc ggacatcgtg cacacaggaa cctacgccgc
     3241 cggtgcgctc tgctcgacag cgaaggacat gattgcatgg ttgcaggcgc tgcacggtgg
     3301 caaggtgctg ccaccgcgct cctacgcgga aatgatcgcg ccctcgaaac tcaacgatgg
     3361 aacgccgctc cgatactcgc tggggaccga aatcggggag gaccgtctcg gtttccggta
     3421 catcggacat ggcggcggcg gcttcggatt ttcgtcgcag gtgaggtggt atcgcgacgg
     3481 gcagttgtcg atagtcgtgc tgacgaacag cgaaccggac aatatcacag tggttaccga
     3541 ggatctcgca gccgcggtgc tgccaccgcc gcgtcgggcg ggcccgttca agggcgacgc
     3601 atcgctgctc agcggcaaat acaaggggcc cggacgcggc accgaaatgg tcatcgatgt
     3661 aacgcacacg ccggagggga tggcgttttc gttcgacggc gccgctgcgg ttccattgcc
     3721 atgggtcgag agttggacgt tccgccgcgg tagctcgcta ttgaccttcc gccggagcac
     3781 gaccactggt cccgccacgg aactgcggtt cgacacggcc ggcgattact tcattctgaa
     3841 gcggcagtaa atagccatcc gctatttact taccgggagc ggatggccga tcgctgacgg
     3901 ctgctcgctc cgctgtccct gggcgccctg caccacttcg aggaatgcgc gggccgcatg
     3961 gcttagcgca cgccgcgcgg ggtatagcag gcggatcttc cgctccatgt tgatttcccg
     4021 gacgttcact tcccaaagtg tgccctgctg gatttcccgc tccacgcaca tcttgggcag
     4081 gaaagcgacc ccttcgttgc gttgcaccat gcggcgtatg gtttcaatgg tgggcatctc
     4141 cacgtccatg ttgagtggaa ctttatgccg ctgaaattcc cggagaactc tttcgcgata
     4201 gggagagaga acgttatggg cgatgaaggt ctccattccg agttccgaga tcgagatcgt
     4261 atcgcgcccg gcaaaacgat gatggggcga gacgacgaag gcgagatggt cggtgaaaat
     4321 gacgctggac accaggcgat catcctccgg atcgaagctg atcacgccca attcgaggtc
     4381 tccgtcgatc agttgcgccg gtattctact cgaaaggctg cgtctgacct ggactttgac
     4441 cttgggatac agcctcctgt actcgacgat gtgctgcatc agatacagcg agctcgattc
     4501 attcgcgccg atcaccagac gtcccgcgga atggtcccgc agctccgcca gcgaattctc
     4561 gagatcccgc tccaggttct caaagcggcg ggcgtattcg agtacgatgc gcccggcgtc
     4621 ggtcaacagc aattctttcg cggaccggtc aatcagcttc tcgttcagtt ctgtctccag
     4681 cctctggaca gccagggaga tggcgggctg agtccgcagc atcttctctg cagcgcggga
     4741 aaagctcttc tcggtggcaa cggcttgaaa aaccttgagg gtaaatagtt ccatcgctat
     4801 tgataaatcc agtttatcac atgagttaaa attatagatt tttctaaaac aataaatttg
     4861 ctaaatcatt gaggctctcg cataatgaga acgtactcag gcgttggaga aacaggccag
     4921 cgccggagct aagctaccgc ttttaagtaa cggccttctg gctatcccgc ccgaaggccg
     4981 ttgctgtaaa ggggagtccc tcacaactcg tgcctttagg aagattcatg aacaaccgcg
     5041 tatacatctt cgacacgacg ctccgggacg gtgaacagtc gcccggctgc agcatgaacc
     5101 agcaggaaaa gctcaaacta gcacacaagc tggttgagct cggtgtggac attctcgaag
     5161 caggattccc tatcgcttcc gagggcgatt tcgaggcggt ggatgccgta agcagggaat
     5221 ttccgtgggt tcaggtagcc gcgctggcgc gggcctgcac cttggacatc gaacgggccg
     5281 cgcgcgcgct ggaacacgcg aagcggccga gaattcacac gtttatcgct accagcgaga
     5341 tccatctgaa gtataagctt cgcaaaacgc agcagcaggt gctggatgag gccgttgccg
     5401 cggtggaact ggcgcggcgg tatgctgatg atgtggagtt ttcggcggaa gacgcgaccc
     5461 gtacggatat ggattttctg gagaagatct cgcgggccgt tgtcgcggcg ggcgcgagaa
     5521 cggtcaatct tccggatacg gtgggttttt ccgttccaga cgaatacggt gaactcatcg
     5581 ggcgcatggt ccggagtttg ggcgattcgg ccatcctcag cgtacattgc cacgacgatt
     5641 tgggactggc ggttgcaaat tcgctggcgg ccgtaaaagc cggtgcccgg cagatagaat
     5701 gtacgatcaa cggcatcggc gagcgcgcgg gcaattgctc gctcgaagaa gtcgtgatgg
     5761 cgatcaatac caggcgggac cgtctgcctt accataccgg tatcgtgacg gaacatctct
     5821 tccccgcaag ccagttgctg acggaaatga tctccttcgg gccgcagccc aataaagcaa
     5881 tcgtcggcga aaacgcattc gcgcacgaag cgggaatcca ccaggacgga tatctgaaag
     5941 agcgttccac ttacgagatc atcgatccga agtcggtcgg cgtccccgag ggcaggctgg
     6001 tgctgggaaa gcacagcggc cggcacgcgc tgaacaagcg ctgccaggat ctggggttcg
     6061 aactgacgcg cgacgaactg gatctcgtct ataagcagtt catcgcgctg gctgatcgca
     6121 agaagggtgt ccggaacgaa gaaatcgctg tattagtccg gcaggaaatc gcccgtgtgg
     6181 ccactgccgc cgactgactt tctagtccac caactctcaa atgaatctta aagtcttgat
     6241 cttacccggc gacgggattg gtgtcgaagt aacccgtgaa gccgtccgcg tgctggagca
     6301 cgtatctcgc aagtacaagc atacgctgga gatttccgag gcattattag gtggggccgc
     6361 cattcaccag agtggaacgc ctctacccga caagacggtg gagctggcct tgaaggccga
     6421 tgcgaccctg ctcggcgctg tcgggttgcc ggagttcgac aatgtccctc ccgaaaaacg
     6481 accggagaag gggctgctcg gcatccggaa agctctcggt gtgtttgcga atctccggcc
     6541 ggttcgcacg tattcatcgt tgctcgaatc gtcgcctctg aaaaaccacc tgatcgaagg
     6601 cacggacatg attatcgtgc gtgaattgac aggtggaatc tattacggca ctccacgcgg
     6661 tatttcgggg gagggcgctg agaccacggc agtgaataca atggtctata cacgcgccga
     6721 aatcgaacgc gtagcgcgaa tggcttttcg tcttgcgcgc ggacgccgga aaaaactgac
     6781 cagcgtcgac aaatcgaatg tcctcgaaaa ttcgcagctc tggcgcaagg tggtaacgga
     6841 agtggccggg gaattcaacg atgtcgcgct ggaccatctg ctggtggaca actgcgcgat
     6901 gcaactggtg ctcaatccgc gccggttcga cgtcgtcctg acggagaaca tgttcggcga
     6961 cattttaagc gacgagggcg ccgtgctcgc cgggtcgatc ggaatgttac cgtccgcgtc
     7021 aatcggcgag cagcgggagt ccggggcctg ggtcgggctc tatgagccgg tccacggctc
     7081 ggccccggat attgccgggc agaataaagc caatccgctg ggcgcgatcg ggtcggtggc
     7141 cgcgatgctc gaatatagtt tcggcctgaa gcaggaagcg gaggccgtca atcgcgcgat
     7201 cgaaacggta ttggggcagg gcagggtaac cgcggatctg aagcccaagg gcgcgcccgc
     7261 caccacttcc gaggtgggag aagcggtctg ctccgcgata taggtagata gagaagggaa
     7321 agaaagaagt agtgccgatg agaaagcctc gaacagttat cgagaaagtt tgggattcgc
     7381 acgtagtgac cgaacagccc ggcgcgccgg cactgcttta tatcgacctg cacctggtgc
     7441 atgaagtcac gtcgccgcag gctttcacgg gacttcgcgc gcgcgggctg aaggtccggc
     7501 gccctgacct gacactggcg accacagacc acagcattcc caccacgcct cgtactttcc
     7561 cgattgtcga ttccgtggcg gccgcgcagg tagcgcaact ggaaagtaat tgcgccgagt
     7621 tcggtatccc gtgctacgga tttcatagcg aaaagcaggg catcgttcac gtgatcggtc
     7681 cggagcaggg cctgacgcag cccgggatga caatggtgtg cggcgatagc cacaccgcga
     7741 cgcacggcgc cttcggcgcg ctcgcattcg gcatcggcac cagcgaagtg gagcacgtgc
     7801 tggcaacgca atgcctgctt cagcgcagat cgaagacctt tcaggttcgg gtggacggag
     7861 cgctccggcc gggagtaacg gccaaggaca tcattctggc gttgatcgcc cgcattggcg
     7921 taggtggagg cacgggctgt gtgttcgaat acaccggcag cgccatccgc gcgctttcga
     7981 tggaagagcg catgaccatc tgcaacatgt ccattgaagg gggcgcgcgt gccggtatgg
     8041 tggcgcccga cgacactact tacgagtaca tgtgcggccg gcccttcgct ccaaaaggca
     8101 aagagtggga ggccgctctc gcccgctggc ggcaactacc gacggacgag ggcgctactt
     8161 atgatctcga ggtgactctt gatgcggcgg aattggagcc gatgatcacc ttcggcacaa
     8221 atcccggcat ggggattccg attaccggtc cggttcccga cccatccttg ctgaacgatg
     8281 tgcaggagcg cgactccctg gccaaggccc tgcggtacat ggaccttcaa cccgggcgcc
     8341 cgctgctggg gcatcccgtc aacgtcgtct ttatcggcag ttgtacgaat tcaagaattt
     8401 ccgatctgcg ggcggccgcc ggcgttctga aaggtcgcaa ggtgaacccc aacgtgcgcg
     8461 tgatggtggt gccgggctcg caggagatca agaggcaagc ggaaagcgaa ggactggacc
     8521 gcgtgttccg cgaatccggc gcagactggc gtgaagcggg ttgttccatg tgcatcgcga
     8581 tgaacggcga ccagcttcaa ccgggcgaat acagcgtctc caccagcaat cgtaactttg
     8641 aaggccgcca gggcaagggc gggcgcacat ttctcgccag cccccttacc gccgcggcaa
     8701 ccgccattac cggtgtagtg accgacgtga ggacactcct gtgaagaagt tcactccatt
     8761 tgagagccgg ctggttccat tgccggtgga caacgttgat accgaccaga tcatccccgc
     8821 gcgttttctg aaaacgattt cgaaggaagg gcttggcgat cagttgttct acgactggcg
     8881 ctatgatgcg gcgggtgcac caaaaccgga cttcatcctc aaccagccac aaggaaagca
     8941 ggggcagatt ctgctggcgg gcgacaactt cgggtgcggc agttcccgcg aacacgcgcc
     9001 atgggcgctg acgcagttcg gtttccgcgc tgtcatcagc acctcatttg ccgatatctt
     9061 caaacagaac tccctgaaga attcgctgct gccgatcgtg gtgccgcgcg atatccacgc
     9121 cgcattgttc gagctcgtct ccagcgatcc cgcagccacc atccgcgtgg acctggcggc
     9181 gcaaaagatc gtgttgcccg acggccgtga agtcgagttt ccagtggacg cgtttgctaa
     9241 gcactgtctg ctggaaggcg tggacgaact cggattcatc ctgcaacagg agccggccat
     9301 cgtacggtac gaagcgcagc gggtgagttc actcgatacg cgtgccggct aatcgccggc
     9361 ttcggtggtt tccacgtttt acacggaaag gggagccatg atcatcgttc atgttttcgc
     9421 ccacgtgaag ccggagcacg tccgcgcgtt ccgtgaggct acggcggaga atgcccggaa
     9481 tagtgttcag gaaccgggag tggcccggtt cgacgtcatt caggagaagg acaacccaac
     9541 gcggttcatg ctggcggaag tgtaccggac tcctgaagcg cgcgcgcagc ataaagagac
     9601 acggcattac caggtgtggc gcgatacggt cgcgcctatg atggccgaac cgcgaaccag
     9661 catcgaatac tccaacgtct ttccgtccga ccaggactgg taagccgatg tctttcgagt
     9721 ttgcgactgc ctcccgcatc gtgttcgggc gcggcgcggc ccgcgagatc atgccggcgg
     9781 cgcgccgcat gggcgcccga accctggttg tcaccgggag gcatccggag cgcgccggct
     9841 ggctcctgac ttctttgaag gctgcgggcc tggagccggc ggtcttcccg gtcgatggcg
     9901 agcccacgat cgattcggtt cgcaacggtg tcgaacttgc gcgggagcgc gcttgcgacg
     9961 tcatcatcag tcttggcggc ggcagcgcga tcgatgcggg caaaggcatc gccgcgcttc
    10021 tggcgaatgg cggtgatccg ctcgattacc tggaagtggt tggaaaaggc caggccttgg
    10081 agaaaccgtc cgcgccgttc atcgccgcgc cgacgacggc gggcaccggg tccgaagtaa
    10141 ccaggaacgc tgtactggcc tcaccgccgg acaaggtgaa ggccagtttg cgaagcccct
    10201 tcatgctgcc ggaactcgcc gtggtcgatc ccgaattgac cgtggatgta cctcccgatg
    10261 tcacgacctt caccggcctg gatgcattga cacaggtgat tgaaccctac gtctcggcac
    10321 ggcgaaacgc catgacggat ctgttctgtc tggaaggcat gcgccgcgtg agccgctcgc
    10381 tcggccgcgc ctatgacgat ggcgcctcga tcgaagcgag ggaggacatg gcttttgcca
    10441 gcctcatggg cgggttgtcg ctggctaacg cggggctcgg tgtcgtacac ggttttgccg
    10501 cgcctatcgg cggaatgttc gacgcccctc acggagcagt ttgcgccgcc ctgttgccgc
    10561 acggcataac ggcaaacatc cgggcgttaa gcgaccgcgc cgaagatcgg gaagtgctgg
    10621 atcgctatca aacggtggca cgcatcctga ccggagatgc gggcgcctcg gccgaagatg
    10681 gcgcggcttg ggttcgggag ctttgccgcc gtttgcgcat tcccggactc gcggcctacg
    10741 gagtcgacgc tgccgcgatc cccgtcctgg tggaaaaggc ttccggcgca agtagcatga
    10801 aggcgaatcc gctggtcctg acgcgcgagg aattgacaga caccctcgag cgggcgcttt
    10861 aatttccgga aggcgactgc ccgcgcggca cgggcttgcc gtaaatctcg atccaatcca
    10921 cgcgtgtgca ggagatgctg ccgccgcccg tcatcagatc ggtccagcca atctcgtcca
    10981 cgcggctcag atcgggattg tctacccagg ctccttcaat gattttgtcg atgtccaatc
    11041 tgcgccagcg gatatcctgg atattgaatt cgcgctcgcg ccagtcgaga gagggaccgt
    11101 cataggcatc gctcaccagc cacgtgccgt tcgcaagttt caggatgatg cgggcgccgc
    11161 gaaaaccggc ctgcttgctg cgccatttca gtttcgattg cccgcgaagg tcgacgtagg
    11221 acgtcttgtg cttcagagca atcgcacaat tgcccacgca ctcgcctgtc cagatgtaaa
    11281 agggatcgtc ggccggtttg tcgtgattac tcttcttaat gccttctttg ccggggccgt
    11341 agagcgtgaa ggtcagattc gaattggcca ggtggtccag cgtgacgggc tttgcgggtg
    11401 gcgtctcttt ccagtcctcc cgaaaaaaca ggcccgggcg gtcctgagcc gcggcggcca
    11461 gtgtgaagaa taagaatgaa ctcaataaca gcttgaacat gatgcttcct tgtctatctt
    11521 aatgattatc ccagctacgc attctgtgcg gcgctggtcc cccgctggcc cggcatggcg
    11581 gcccagaaca ccaggtacac aatgaacccg gcgccggtaa agatggccac gatcagccac
    11641 acgattcgga cgagcgtcac gtccacgccg agataatgcc ccacaccggc gcacactcct
    11701 ccgattttct tttcatcgag aggccggacg agatgtcgct ccgttggagt ccagggcgtt
    11761 gaaccggccg gggtgtcgag aggttgaacc ggcatcccgc aacgcgtgca gaaacattgg
    11821 ccctcttcga gcttcgatcc gcatcgcgtg cagtacattc cgaaacgagc ctatcgcagt
    11881 cgccggggag ggtgcaaacg gggtgttggt agcggccctg agtcgcgcgc gtcagcatgc
    11941 ctgatccgga acttgcgttt aaaccgttat cgggaacaag ctgtttctgc gtggaacacg
    12001 atgacgttgg cacctgcatg tagattcgtt tgaccaatct gaatgtggga agcagacggc
    12061 gcctgggaaa atgagactcc agtcgccgcc accaatcctg ctggagagat cgcgagaccc
    12121 tcgagcagat ttcgttcctt gttaccgaag taacttgcga aacagagctt tgtcaggtcc
    12181 gcagaaaagg cggcaacgaa gccatccccg ttgccgctgc cgaatcgtgg ctgcccggca
    12241 tttctggtgg gcaaatcgtc agaataagtg attccagcca accagatatt cccgtgccga
    12301 tccactttga tatcgccgcc gtcgtacccg ctctcatcgt cattcgagcc tccgaaatag
    12361 gttgaccgaa tctctcgtcc atgccgcatg ccaatagacg ctatgaacgc atccctcttg
    12421 ccacggttgc gcggttgata ggatgcgctt acacccggca gatccaccga gttggtgatg
    12481 ccggccatca ccggatttcc cctcatatcg agtgcaatgc cccatccgct gtcctcgccg
    12541 ctcccaccga agaacgtcga ggatgtcatt tccagcgacg ggagggcgag cctggtcagg
    12601 aacaaatcac taggcccggc aagcgcattt tgcaccggat cttttaccgg aaaatccgga
    12661 gaggtggtcg agccgacagc gtagatgccc cccgctcgat cgagtacgat tccggttagc
    12721 ttctctcctt ttcgaccgcc aaagatctcg catgagatgg catgctcgac cgctcggaac
    12781 caacacacga aagcatcatc ctccgtggta agcctattgt tgccctgtgc tggaaaatca
    12841 ctcgacgagg ttacgcctcc aagataaacg ttgccccgct cgtcaagatc caacccatta
    12901 ccgaggtcgt cgcgcgcccc accgatcaac gtggcatata cgatttcgcc gcccggcgcg
    12961 accttgacca gaaacgcatc gctcccgccg gctaacttcg gctgcaatgc gtccttggta
    13021 gttgggaaat ccggcgactt cgtcaacccg gtcgtatagg cgtagccatt cttgtcgact
    13081 ttgattcgga gagctgcgtc gaacgaacgg ccgccaaatc gagtggcaaa gacgagcttt
    13141 ccggtgcgcg gattcagccg gagcacatat gcatccattt catccgcggc cttcgcacca
    13201 cgcacgtcaa tcggaaggcg gtcgtagggc gagtggcaag cgagatagat gtcttcatca
    13261 ggaccgaagg ccacatcgtc gcagtccgcg atttccggtc gatgaacctg taaaggagca
    13321 cgctggctct tgagggtggc caccgttgcg caacaaacgg tcaatagcca aacgagtgat
    13381 ctcatgttgg tcagtctccg gccctggcca tccaccatac tccccgccac aattgccttc
    13441 taatgccgct ggtaatgccc tatgtactcc gcctgcgcca gggtgtgccg ttgtaaggcc
    13501 cggtcgaggt ctgcgcggcg ggcgccgtcg gggattccca acgaatccgt gtccatcgca
    13561 tacaaccgga agaagtagcg atgcggatcg tgccctttcg gaggacaggg gccgccgtag
    13621 ccctgctttc caaagtcgtt gattccatcg cggccaagct ggcctggttt gaagccctga
    13681 gcgagcgaat ggatatttgc cgggatatcg tacagcagcc agtgattcca ggtgccgcgg
    13741 ggagcgtcgg gatcgtcgac gataagggcg acccccaata agcgttaact taaaatttga
    13801 cttcactgaa cgcctgtgag atgtttgagg cgtgaatgct tccagcgaag atctggcagt
    13861 cgaaaattgg gaggtccttc ggacatttct gcccaagggc tgggaggaaa tggctcgcaa
    13921 gagcggagca ctcag
//
LOCUS       DASTMK010000061         9461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_387142, whole genome shotgun sequence.
ACCESSION   DASTMK010000061 DASTMK010000000
VERSION     DASTMK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9461
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387142"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFQ79_02535"
     CDS             <1..1333
                     /locus_tag="VFQ79_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138553.1"
                     /translation="SIDKVSRVFPTNYVDIVEASSIGLPGNTLIRTDRNNFAPRLGLA
                     WRPLGPNTVVRGGFGVYFDVVPRMLTMAGVPFLLNEAPFNNPADNPVVVLPNVFPSAG
                     AAGPSSVSLPAAVNPDLKIPYSMQYNLTVEHSRWNTGFRASYIGTNTRQGDYSYNYNS
                     PVPDARLFTEKPRPFPQYPAITYFTNGAGHQFHSFTAEVERRMARGLQFQSSWVWARD
                     IGDLERGQSLENPFDRERERSVWPDIPTHRFTTNWIYELPFGKGRPFLGAANRAVNAI
                     VGGWDLSGIYSYYSGQFLTPLWTGPDPTGTAFTTSGRAVVTIRPDHLQDANLSGDQRS
                     INRWFDPAAFGAPPIGRFGNAAKGVIKGPWVNIWHMGLFKSFMFTERARLRWELTATN
                     IFNHPNYSNPATNISQAGAVGVISGVGGVNGASTGDQPFARSFRMGIRFEW"
     gene            1423..2118
                     /locus_tag="VFQ79_02540"
     CDS             1423..2118
                     /locus_tag="VFQ79_02540"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEU0138554.1"
                     /translation="MVFDPMYLLFILPGLALSLWASFRTKSAFNKYSQIRSARGMTGA
                     QAAQLLLDGAGIRDVGIKVSHGLLSDHYNPVNKTLNLSEEVYGSTSVAAIGVACHEAG
                     HAIQHAVHYKPMWLRSALVPTANIGSSIGYFVMLLGLMFASTNMVLVGAILFSMVLLF
                     QVVTLPVEFDASNRAKRLVLEQGIVTAQERQGIDKVLNAAALTYIAAAVSTLLTLLYF
                     LMRAGLLGGRRSD"
     gene            complement(2060..2830)
                     /locus_tag="VFQ79_02545"
     CDS             complement(2060..2830)
                     /locus_tag="VFQ79_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0138555.1"
                     /translation="MNNIASHLLCAALLLMSGTELAAGTITAFGSTSIPSGSTGSIGP
                     VGLTPAPNNDNISVASSNAISYNIFFNSFGTLDVEYVVSDSGGTTEYTVTQSLLNNSG
                     QTWTGFQFVLGFGLGTNFAPVSSLGSLDFDTPDGDPAPVSSRFSSLDHQPTTLIWSGA
                     VVPLFGTTTFSFAIDVPDGVSGLNPNGFNRFTLRQFPIAPTPVPEPGTLALLGAGLAG
                     IGLKLRNGAARVSKRKLLTTNRCGGRPADPRASGSKVA"
     gene            2945..3715
                     /locus_tag="VFQ79_02550"
     CDS             2945..3715
                     /locus_tag="VFQ79_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0138556.1"
                     /translation="MEEVSAIPMIEAVGLNKIYGNFAAIKDVTFAVPKGQVAAFLGPN
                     GAGKSTTMKILTGYLSPTSGQARIAGIDVTTDRIRAAERLGYLPENGPLYPDMTPLGL
                     LKFFGRARGLAGARLRSRIEHVVERCALGAVLEKNIRKLSKGYRQRVGMAQVLLHEPD
                     VLIMDEPTSGLDPNQIREVRKTIRELGSEKTVLLSTHILQEVDAMADRVILVSRGRIV
                     FDGTPAAMQTGYASLEDAFYALTEAAGKEDGTCVAAGA"
     gene            3694..6288
                     /locus_tag="VFQ79_02555"
     CDS             3694..6288
                     /locus_tag="VFQ79_02555"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEU0138557.1"
                     /translation="MRSRGRVIRAIAVRELDGYFSTPTGYVFITLFVFLSGMAAFWQE
                     RFFATNLANLDQLNRVFPYLLMMLVPAISMSLWSEEKKQGTDELLLTLPASNLEIVLG
                     KYFAAVAIYTTALIFSLSHVVVLSWLGNPDPGLMASTYLGYWLMGIALLSLGMLASQL
                     TDNLTVAFILGVVLCAFPVLLNHAGVILSGDMQRVAERLSVPEQFRDLSNGVITITPL
                     VYFGAFAVAGLYLNVALLGRRRWPTGPKSPFMRLHVLARAVSLMAIVAGVTLLAGGVR
                     FRVDATAEQIHSLSDQTRALLNGIDASQPVFIHAFISPDVPRGYVDARTNLVAMLREF
                     GAVGRNRVHTRIVQTVKYSPEAREAQERYNIRPYRVPVTEESARVMNEIFLGVAFTCG
                     SEEFVIPFFDKGLPVEYELMRSIRVVSRADRKKVGVLDTQARLFGGFDFQKRAQNQEW
                     SIVQELRKQYEVVQVSPDADYPEGLDALVAALPNTLTQPQLDRLTAYIKEGKPALILI
                     DPMPAFNLELAPQDIASSEPFGAPPSSPRANLRPLLQALGIEWQTNRIAWDGHNPHPQ
                     LKTLPKEFVFVGKGFNQKEPVSAGLQEMVLLYPGLLKPAGAGEFIPLLETSEDAGTVR
                     WENLVQQSMFGVSMNPDPPRAPEQKPGVLAARIGKPANAIVVADVDLIGEQFFELRRR
                     GVENLNFDNVTFILNAVDQLAGDQSFIALRKRRPKHRTLEAVEARTRTYEAQRLRETE
                     MAEATAEQRLKEAQARLDRAVREIEQRPDLDERTKQIMISNVQSVENRRLTVARANID
                     DEKQRQIENSRADMENSIRGIQNTIKLLAVALPPIPAFLVFVLVTIRKLRLERMGVPV
                     DRLAEERA"
     gene            6288..7517
                     /locus_tag="VFQ79_02560"
     CDS             6288..7517
                     /locus_tag="VFQ79_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEU0138558.1"
                     /translation="MRETLKTAAFAGAALVLVLAAGIVEPDTVTPKILDDQGEAFYPK
                     FTDPKAPKTIEVIDYDEETATARPLKVQFEKGRWVIASHHNYPVDAGDRLAKTAGALV
                     DLRKDVVRSDSPQDHGELGVVDPLDQKVAALTGRGKRVTLRDEHGEVLADYVLGKPVE
                     GKEGFRYVRVPGEKRTYAVKTAADPSARFADWVDPNLLRIAASSIRKVTVHSYTIDEM
                     IGRLVNAVSIVLTREDGQWRASGDANPSKQVINEMVSTLDGLRVVDVRPKPPSLAQDL
                     RKGQLQLSLETAVSLRQRGFLLSPSGRILANDGEMAVETSDGLVYTLRFGEVATGGPD
                     SRSAAGAMENRHLFVTAHYKPQSPEADTAAGERRARSLNERFADWYFIIRGQDVQKLK
                     MQKATLVSGAAQPKPEL"
     gene            complement(7529..8686)
                     /locus_tag="VFQ79_02565"
     CDS             complement(7529..8686)
                     /locus_tag="VFQ79_02565"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU0138559.1"
                     /translation="MKLVRLPLLLAGLVLPFTTMLSAAPQNTNDDYLVYIGTYTGKGG
                     EGSRGIYVYRFDSSSGKLTPAGLAAEIPSPSFLAIHPNQRYLYSVRESGKEGSVSAFE
                     IDKSTGKLKPLNTVSSKGGGPCHLVVDKTGRNVLVVNYGSGSTAVLPVKQDGSLEEAS
                     SFIQHSGTGAEPRRQKGPHAHSVNMDANNRYAIVADLGLDDVLVYRFDPKKGLIEPND
                     PPYAEVAPGAGPRHFAFHPKGRFGYVINEMGNTVTAFLWDGKKGTLTEIQTITTLPKD
                     FSGTSYTAEVQVHPNGRFLYGSNRGHDSIAVFSVDPSKGTLSPIEYVPTQGKFPRNFG
                     IDPTGSYLIAANQDGNNLVVFRIDSKTGKLTPTGQVEEVGRPVCVKFLPLK"
     gene            complement(8802..>9461)
                     /locus_tag="VFQ79_02570"
     CDS             complement(8802..>9461)
                     /locus_tag="VFQ79_02570"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEU0138560.1"
                     /translation="RLDIAANQPIPGVHWGTRLVLPLMEVRVPFHLNQMELDSITRGV
                     KDPDFNDLEKAARQTALFEEQAIFNGIPDTPIKGIIPSCSHEPIRLQGGLESLPHAVA
                     SGLKLLQEAGIGGPFHLVLGEAAFFTLMQAIDEGYPIYKVVENLVEGRIHRSPALSGG
                     VLLSGRGGDFELTIGQDISIGYAAHDKDQVELYFTESFTFRVLETAACVPIELQQEPQ
                     I"
BASE COUNT         1869 a         2836 c         2837 g         1919 t
ORIGIN      
        1 gtccatcgat aaggtgagcc gcgttttccc aacgaactac gtggacatcg tggaggcgag
       61 cagcattggc ctgccgggaa acacgctgat tcgcaccgac aggaacaact tcgcgccccg
      121 attaggcctt gcgtggcgcc cattgggccc gaacacggtg gtgcgcggcg gcttcggcgt
      181 ctacttcgac gtcgttccgc gcatgttgac catggcgggc gtgccgttcc tgctgaacga
      241 ggcgcccttc aacaaccccg ccgacaaccc ggtagtcgtt ctgccgaacg tcttcccgtc
      301 cgcgggcgcc gccgggcctt cttcggtgtc tctgcccgcc gccgtaaatc cggatctgaa
      361 gattccgtac agcatgcagt acaacctgac cgtggaacac tcccgctgga acaccggatt
      421 ccgcgcgtcg tatatcggga cgaacacccg gcagggcgat tactcgtaca actacaattc
      481 gccagtgccc gatgccaggc tctttacgga gaagccgcgt ccgttcccgc agtacccggc
      541 catcacctac ttcaccaacg gcgccggaca tcagttccac agctttaccg ccgaggtgga
      601 gcggcgtatg gctcgcggcc tgcagttcca gagttcctgg gtctgggcgc gcgacatcgg
      661 cgacctcgag cggggccaat cgctcgagaa ccccttcgat cgggaacgcg aacgcagcgt
      721 gtggcccgac ataccgacgc accgtttcac tacgaactgg atttacgaac tgcccttcgg
      781 taaagggcgt ccgtttctgg gcgcggccaa ccgtgccgtt aacgcaatcg tcggcgggtg
      841 ggatctcagc ggcatctaca gctactattc cggtcagttc ctgacgccgc tgtggaccgg
      901 acccgatccc acggggaccg cttttaccac aagcggacgt gcggtagtca cgattcggcc
      961 cgaccacctt caggatgcca atttatcggg agatcagcgc tcgattaatc gctggttcga
     1021 cccggccgcc ttcggcgctc cgcccatcgg ccgtttcgga aacgccgcca aaggcgtaat
     1081 caaggggccg tgggtgaaca tctggcacat gggcctgttc aagtcgttca tgttcaccga
     1141 gcgcgcccgc ctgcgctggg aactgacggc gaccaacatc ttcaaccacc ccaactacag
     1201 caaccccgcg accaatatct ctcaagcggg agccgtcggg gtgatttccg gagtgggcgg
     1261 cgtcaacgga gcctccacgg gtgaccaacc cttcgcacgc tctttccgca tgggcatccg
     1321 cttcgagtgg tagcccgctc gtagcgtgga gaagccggtg ttgtttccgg cttctccatt
     1381 atgaaaaaga tataattaaa gatgagggag tgaagggaac gcatggtttt cgatccaatg
     1441 tacctgttgt ttatcctgcc tgggttggct ctgtcgttgt gggccagctt ccgcacgaag
     1501 tctgccttta ataagtactc gcagatacgg tccgcgcgcg ggatgacggg agcgcaggct
     1561 gcacaactcc tgctggatgg cgccggaatc cgcgacgtcg gcatcaaagt ctcgcacggg
     1621 ttgctgtcgg accactacaa cccggtcaac aagaccctga acctgtccga agaggtatac
     1681 ggctccacat ctgtggcggc tatcggagtc gcttgccatg aagccgggca cgctattcag
     1741 cacgctgtgc attacaaacc catgtggctc cgctcggccc tggtccccac cgcgaacatc
     1801 gggtcatcca tcgggtactt cgtgatgctg ctgggcctca tgtttgcttc cacgaacatg
     1861 gtgctggtgg gcgccatcct gttttccatg gttctgctgt tccaggtcgt gactctgccg
     1921 gtggaattcg acgcgtcgaa ccgggcaaag cgcctggtgc tggaacaggg aattgtgaca
     1981 gcgcaggagc gccaggggat cgataaggtt ctaaatgccg ccgcgctcac ttatatcgcc
     2041 gcggccgtga gcacgctgct cacgctactt tacttcctga tgcgcgcggg tctgctgggc
     2101 ggccgccgca gcgattagtc gttagcaatt tccgcttgct gacgcgcgcg gctccgttcc
     2161 gtaacttcag tccgataccg gccagcccgg cgccgagaag agccagagtg cctggttccg
     2221 gcacgggagt tggagcgatc gggaattggc gtagcgtgaa ccggttgaag ccattcggat
     2281 tcagtccgct cactccatcg ggcacatcga ttgcgaagct aaaggtggtt gtgccgaaca
     2341 aaggcacaac agctccgctc cagatgagag tggtcggctg atgatcgagc gaggagaatc
     2401 gcgacgacac cggagccggg tccccgtccg gcgtatcgaa atcgagcgag cccagggaac
     2461 taactggtgc gaaattcgtt cccagaccaa acccaaggac gaactggaac cccgtccagg
     2521 tttgccctga gttgttgagg agagactgcg tcactgtata ttcggtcgtc ccaccggagt
     2581 cggaaaccac atattccaca tccagcgtgc cgaatgaatt gaagaaaatg ttgtacgaaa
     2641 ttgcattcga gctggctaca gatatgttgt cattgttcgg agcgggggtg agtccaacgg
     2701 ggccgatgct gcccgtggaa ccgctgggaa tagacgtgga gccgaaagcg gtgatcgttc
     2761 ccgccgccaa ttccgttccg gacatcagga gcagcgcggc acaaagcagg tgcgatgcga
     2821 tgttgttcat gaggagttgc ccaggaggga cagtatatat aattttgcgg acgctgtccg
     2881 tccgatggta aatgtatagg tatcacgccg gttgtgtagt agcgccacgc aggaaactcc
     2941 tagtatggaa gaagtgtccg ccatacccat gattgaggcc gtcggcctca acaagatcta
     3001 cggaaatttc gcagccatca aagatgtgac cttcgcggtt cctaaaggcc aggtcgccgc
     3061 gtttctcgga cccaacgggg cggggaaatc cacgacgatg aaaattctca cgggatatct
     3121 gtcgcccacg tccggacagg cgcgtatcgc cggtatcgac gtcactacgg accggatccg
     3181 ggcggcggaa cggttaggct atctgcccga gaacggaccg ctatatcccg atatgacgcc
     3241 cctcggattg ctgaagttct tcgggcgcgc cagaggactc gccggagcgc ggctccggtc
     3301 ccgaatcgaa cacgttgtcg aacgctgcgc gctgggcgcc gttctcgaaa agaacatccg
     3361 caaattatcg aagggctacc ggcagcgggt cggcatggca caggtgctac tccacgagcc
     3421 ggacgtactc atcatggatg agccgaccag cgggctcgat cccaaccaga tacgcgaagt
     3481 acgcaagacc attcgggagc tgggcagcga gaaaacggtc cttctctcca cccacatcct
     3541 gcaggaagtg gatgcgatgg cggatcgcgt catcctggtg agccggggaa gaatcgtctt
     3601 cgacggtacg cctgccgcga tgcaaaccgg ttacgcttcg ctcgaggatg ccttttacgc
     3661 actgacggaa gcagccggga aggaggacgg aacgtgcgta gccgcgggcg cgtaattcga
     3721 gccatcgccg tacgggaact ggatggctat ttcagcaccc cgacaggcta tgtcttcatt
     3781 acgctgttcg tttttctgag cggcatggcc gctttctggc aggagcgctt tttcgccaca
     3841 aatctggcca atctcgacca actgaaccgc gtgttccctt acctgctgat gatgctggta
     3901 ccggccatca gcatgagcct ctggtcggaa gagaagaagc aagggacaga cgagctgctg
     3961 ctgacactgc cggcgagtaa tctcgagatc gtattaggca aatactttgc cgcggtagcg
     4021 atttacacca ccgctttgat cttctctctc agtcacgtgg tggtgttgtc ctggcttgga
     4081 aaccccgacc cgggactgat ggcgtccact tacctcggtt actggctcat gggcattgct
     4141 ttgctctcgc tgggcatgct ggcttcccag cttaccgaca acctgacggt ggctttcatc
     4201 ctcggcgtgg tgctctgcgc ctttcctgtg ttgctcaacc acgcgggagt catcctgtcg
     4261 ggggatatgc aaagagtcgc cgaacgcctc tcggtgcctg agcagttccg cgatctttcc
     4321 aacggggtga tcaccatcac tcctctggtt tatttcggtg cgtttgctgt ggccgggctg
     4381 tatctcaacg ttgcgctgct cgggcgaagg cgctggccca caggcccgaa atcacccttc
     4441 atgcggctgc acgttctggc tcgcgccgtc tctctcatgg ccatcgtggc gggtgtgact
     4501 ttgctggccg gcggtgtgcg cttccgcgtg gacgccaccg cggaacagat tcattcgcta
     4561 tcggatcaga cgcgcgcgct cctgaatggc atcgacgcct cgcagcccgt cttcattcac
     4621 gctttcatca gccccgatgt accccgcggc tatgtggacg cgcgaacgaa cctggttgcc
     4681 atgctgcggg aatttggcgc cgtcggccgg aacagggttc acacgcggat cgtgcagacg
     4741 gtgaagtatt ctccggaggc gcgcgaggcg caggagcgat ataacatccg gccctaccgc
     4801 gttccggtta ccgaagagag cgcccgcgtg atgaatgaaa ttttcctggg tgttgccttc
     4861 acctgcggct ccgaggagtt cgtgattccc ttcttcgaca agggactgcc ggtggaatac
     4921 gagctgatgc ggtcgattcg tgtcgtatcg cgcgcggacc gcaaaaaggt cggggtgctg
     4981 gacacacagg ccaggctctt cggtggtttt gattttcaga agcgcgcgca aaaccaggaa
     5041 tggtccattg ttcaggaatt gcgtaagcaa tatgaggtgg tgcaggtctc tccggatgcg
     5101 gattaccccg aaggtctgga tgcgctggtc gccgccttgc ccaatactct tacgcagccg
     5161 caactggacc ggttgaccgc gtatataaag gagggcaagc cggcactgat cctgatcgat
     5221 cccatgccgg ccttcaacct ggagctggcg ccacaggata tcgcatcctc ggagccgttt
     5281 ggcgctccgc cttcctcgcc gcgtgcgaac ctgcggccgc tgctccaagc cctcggaatc
     5341 gagtggcaaa cgaaccgcat cgcatgggac ggccacaatc cgcatccgca actgaaaacg
     5401 cttcccaagg agtttgtttt tgttggcaag ggcttcaacc agaaagaacc ggtcagcgcg
     5461 ggattgcagg agatggtgct tctgtatccc gggttgctga aacctgctgg cgccggcgag
     5521 ttcatcccgt tgcttgaaac cagcgaagat gccggaaccg tgcgctggga gaatctggtc
     5581 caacaatcga tgttcggtgt cagtatgaat cccgatcctc cccgcgctcc ggagcagaag
     5641 ccgggagtgc tggcggccag aatcggaaaa ccggccaatg caattgtcgt ggcggatgtc
     5701 gacctgatcg gcgagcagtt tttcgaactg cgccgccgcg gcgtcgagaa cctgaacttc
     5761 gacaacgtta ctttcatttt gaacgcggtg gaccaacttg cgggcgacca gtcgttcatt
     5821 gcgcttcgca agcgtcgtcc gaaacaccgc acgctcgaag cggtggaggc acgtacccgc
     5881 acgtatgaag cgcagcggct gcgcgaaacg gaaatggccg aagcgaccgc ggaacagcgc
     5941 ctgaaggaag cgcaggcgcg cctggaccgg gccgtacgcg agatcgaaca gcggcccgac
     6001 ctcgacgagc gcaccaagca gatcatgatc tccaacgtgc agtcggtgga gaaccgcagg
     6061 ctcaccgtgg cgcgcgcgaa catcgacgac gaaaagcagc gccagatcga gaacagccgc
     6121 gccgatatgg aaaactccat ccggggtatt cagaacacca tcaagctgct agccgtggcg
     6181 ctgccgccga ttcctgcgtt ccttgtcttt gtgttggtga ccatccgcaa attgcgcctg
     6241 gagcggatgg gagtgccggt ggaccgcctg gcggaggagc gggcctaatg cgggaaactc
     6301 tcaagacagc ggccttcgcg ggtgccgccc tggttcttgt gctggcggcg ggaatcgttg
     6361 aaccggacac ggtaacgccg aagattctgg acgatcaggg cgaggcgttc tatcccaagt
     6421 tcaccgatcc caaagcgccc aagactatcg aagtgatcga ttacgatgag gagaccgcga
     6481 cggcgcgtcc gctgaaggtg cagtttgaaa agggcagatg ggtcatcgcg tcccatcaca
     6541 actaccccgt cgatgcgggc gaccgtcttg ccaaaaccgc gggcgcgctc gtcgatctgc
     6601 gcaaagatgt cgtccgttcc gattctccgc aggaccacgg ggaactgggt gtcgtcgatc
     6661 cgctcgatca aaaagtcgct gcgctcaccg gccgcggcaa gcgcgttacg ctgcgcgatg
     6721 agcatggaga ggtgttggcg gactatgttc ttggcaagcc ggtggagggc aaggaaggat
     6781 tccgctacgt tcgtgtgccg ggcgaaaaac gcacctatgc cgtcaagact gccgccgatc
     6841 cgtccgcgcg cttcgccgat tgggtagatc ccaacctgct tcgcatcgcc gcgtcttcca
     6901 tccgtaaggt gacggttcac agctatacca tcgacgagat gatcggccgt ttagtaaatg
     6961 cggtgtccat tgtcctcacc agggaggacg gccagtggag ggcatccgga gacgccaatc
     7021 cttcgaaaca ggtcatcaac gaaatggttt cgactttgga cggcctgaga gtggtggatg
     7081 tccggccgaa gccgccgtcg ctggcccagg atctgcggaa ggggcaactg caattgtcgc
     7141 tcgaaaccgc cgtttccctg cggcagcgcg gtttcctgct atctccctca ggccgcatcc
     7201 tggctaacga cggagagatg gccgtggaga ccagcgacgg gctggtctac acgctgcgct
     7261 tcggcgaagt ggcgaccgga ggcccggatt cgcgttccgc ggccggcgcc atggagaacc
     7321 ggcatctttt cgtcacggcg cactacaaac cgcaatcgcc ggaggccgat accgccgccg
     7381 gcgagcggcg tgcgcgcagt ttaaatgagc ggtttgcgga ctggtatttc atcatccgcg
     7441 gccaggatgt tcagaaactg aaaatgcaga aggccacgct cgttagcggc gcggcccagc
     7501 cgaaaccgga actgtagccc gccggggttt atttcaacgg cagaaacttc acgcagacgg
     7561 gcctgcccac ttcctcgact tgtcccgtcg gcgtaagctt acccgtcttg ctgtcgatcc
     7621 ggaagacgac caggttgttg ccatcctgat tggcggcgat caggtacgag ccggtcggat
     7681 cgatgccgaa atttcttggg aacttaccct gtgtcgggac atactcgatc gggctcagcg
     7741 tgcctttcga cgggtctacc gagaatacgg cgatgctgtc gtggccgcgg ttggagccat
     7801 acaggaacct gccgttcggg tgcacttgga cttcggcggt gtagctggtt ccggagaagt
     7861 ccttcggcaa ggtggtgatc gtttgtattt cggtgagcgt tcctttcttg ccatcccaca
     7921 aaaacgccgt caccgtatta cccatttcat tgatgacgta gccgaagcgg cccttcggat
     7981 gaaacgcgaa atgcctcggt cccgcgcccg gcgccacctc cgcataggga ggatcgttgg
     8041 gctcgatcaa acccttcttt ggatcgaagc ggtaaaccag cacatcgtcg agaccgaggt
     8101 ccgccacgat ggcgtagcgg ttattcgcat ccatgttgac ggaatgagca tgcggccctt
     8161 tctggcgtct cggttccgcg cccgttccac tatgctgtat gaacgacgag gcctcttcga
     8221 gcgaaccgtc ctgcttcacc ggcagcacgg ctgtacttcc gctgccatag ttcaccacca
     8281 gaacattcct gccggtcttg tccaccacca ggtggcaggg tccgccacct ttcgacgaca
     8341 cggtattcag cggcttcaat ttgcctgtgg acttatcgat ctcaaacgcg ctgacgcttc
     8401 cttctttccc ggactccctc acggagtaaa ggtaccgctg gttgggatga atcgcgagaa
     8461 acgaagggct gggaatttca gccgccaatc cggcgggcgt cagtttgccc gacgaggaat
     8521 cgaaccggta gacatagatc ccccggcttc cctcgcctcc ctttccggta taggtgccga
     8581 tgtaaaccag gtaatcgtca tttgtgttct gaggagcagc ggagagcatg gttgtaaagg
     8641 gtagaaccaa cccggccagc aacaacggga gccgcactag tttcatggct acatgaaacc
     8701 gcatagtgcg tcccgggcgc aagtccatct ccgatccata ccggcaggca tctcaaccgg
     8761 gccgctcagt aaccgagccg cgcgcgcaag caagcggtct tttagatctg cggttcctgc
     8821 tgtagctcga tcggcacgca agccgctgtt tcgagcaccc ggaacgtaaa ggattcggta
     8881 aagtacaact cgacctgatc tttgtcgtgc gccgcgtagc cgatcgagat atcctggccg
     8941 atggtcagct cgaaatcgcc gccacgtccg gagaggagca ctccgccgct caacgcgggg
     9001 ctgcgatgaa tccggccctc taccaggttc tccaccactt tgtaaatcgg gtatccttcg
     9061 tcgatcgcct gcatcagcgt gaagaatgcg gcttcgccca gcacgaggtg gaacggcccg
     9121 ccgatgcccg cttcctgaag cagtttgagc ccgctcgcca ccgcatgcgg cagggattcg
     9181 aggcctccct gcaaccggat cggctcatgc gagcagcttg gaatgatgcc tttgatggga
     9241 gtatcgggaa taccgttgaa gatcgcctgt tcctcgaata gcgccgtctg cctggctgct
     9301 ttctccaggt cattgaaatc cggatctttc accccgcgcg tgatgctgtc cagctccatt
     9361 tggttcaggt ggaagggtac ccgcacctcc atcagcggca gcaccaggcg cgtcccccag
     9421 tgcacaccgg gaatcggctg gtttgccgca atgtccagtc t
//
LOCUS       DASTMK010000062         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_35312, whole genome shotgun sequence.
ACCESSION   DASTMK010000062 DASTMK010000000
VERSION     DASTMK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35312"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VFQ79_02575"
     CDS             <1..1233
                     /locus_tag="VFQ79_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0138561.1"
                     /translation="IFVYRLRSTIKTATHDVRRRQSELVSIVQEGLGAIRVVKAFAQG
                     AFERERLSAKSLESVEAALYARRVRSVLGPVATGLVALGTAAVLWFGARLVLEDAMTA
                     GALVVFLTYLGKLFKPIQDLARASTNIAQAAVGLDRVKAVLDADERLPRSPHTRSLQT
                     VKGRVEFSHVNFGYDPGRLVLTDVSFAAEPGQMIGLVGPSGSGKSTLVSLLPRFYDPL
                     SGSVMIDGHDIRDFTIRSLRRQIAFVLQETQLFYAPVWQNISYGKPEATRDEIIRAAQ
                     LAQAHDFIEALPNGYDTLVGQGGMPLSGGQRQRLGIARAMVRDARIVILDEPTSGLDT
                     GSERLVFEALHNLLRNRTTFVIAHHLNTIRKADCILVMDQGRIVERGTHEELLALGGL
                     YARLQQMEGPVETQSLEP"
     gene            1339..>3090
                     /locus_tag="VFQ79_02580"
     CDS             1339..>3090
                     /locus_tag="VFQ79_02580"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0138562.1"
                     /translation="MVRLAGRLSVSGLLLLAAWGGAQTTSTEVLGTVVDASGAVVPKA
                     TVTLLRVDTGEKRQTLTDASGNYSFPLIEIGAYQVTAEVPGFKTQTKTGIHVELQQKA
                     RVNFTLELGATSERVEVVASGVELRTDDAAIGASIDQKRVVELPTLNRNFASLLVLTP
                     GVQYGNRMGGNVQATGAFPFPGAATTVSANGQRDANQRVTMDGVVATEPLVNQVMFNP
                     SIDAIQEVKVQTATYSAEYGQNNGANVQIALKSGTNDFHGTFYDFLRNDQLDARDYFL
                     NFQVPSGTPERRKNALRRNQFGTWLAGPVLLPGYRGRDRTFWSFNFEGTRETQESVQQ
                     AFWFPEAFRNGDFSALLTPLIRDGRPVRGPIVIFDPVTGEPFRDSAGRITNIIPASRI
                     NRNAQNFINTYLPLPQFVPEDILDVNVIGTVPSILDQNQYFARIDHNLSSKDRIFGRY
                     ATQVSSYVAQSLNPNFPTNYSIRAHNIASQYLRLFSPTVLNEFRFGFNKTDHDQRNPR
                     TYTNFDADSLGVGRFRVAVDNNRKFNELETGIPPTGILQGDGAARVDENGIYQISDNF
                     SFMRGSHSFKTGIEYQRY"
BASE COUNT          654 a          922 c          912 g          602 t
ORIGIN      
        1 atcttcgtct atcgcctgcg gtcgacgatc aagacggcca cacacgacgt ccggcgccgt
       61 caaagcgaac tcgtctccat tgtgcaggag gggctgggcg ccatccgcgt agtgaaagcg
      121 ttcgcacagg gcgctttcga gcgcgagcgg ttgagcgcca agagcctgga aagtgtcgag
      181 gcggcgctct acgcgcggcg agtccgctcc gtactcggcc ccgtggccac cggactggtg
      241 gcgctcggta cagcggccgt gctctggttc ggcgcgcggc tcgtgctgga agatgccatg
      301 acggccggtg ccctggtcgt cttcctgacg tatctcggca agcttttcaa gccgattcag
      361 gatctcgcgc gcgccagtac gaacatcgcg caagcggcgg tgggcctcga tcgggtcaag
      421 gccgtgctcg acgcggacga acgccttcct cgttcaccgc acacgcgatc gctccagaca
      481 gtaaagggcc gcgtcgagtt cagccatgtc aactttggct atgatccggg ccggctcgtg
      541 ctgaccgatg tatctttcgc cgcggaaccc ggccagatga tcgggttggt ggggccgagc
      601 ggcagcggga agtcgacact cgtcagcctt cttccccgct tctacgatcc gctgtcaggc
      661 agcgtcatga tcgacggtca cgacattcgc gatttcacga tccgctccct gcgccggcag
      721 atcgctttcg tgttgcagga gacgcagttg ttctacgctc cggtctggca gaacatctcc
      781 tacgggaaac cggaagccac gcgggatgag atcattcgag cagcgcagct cgcacaggct
      841 catgatttta tcgaagcctt acccaatggc tacgacaccc tggtgggaca aggcgggatg
      901 ccgttatcgg gagggcagcg ccagcggctg gggatcgcgc gggcgatggt gcgggatgca
      961 aggattgtga tcctggacga gccaacttcc ggcctcgata ccgggtccga acggcttgtc
     1021 tttgaggcct tgcacaacct gctccggaac aggacgacct ttgtgattgc acaccatctg
     1081 aacacgatca gaaaggcgga ctgtattctc gtgatggacc aggggcgcat cgtcgaacga
     1141 ggtacgcacg aagaactcct cgcgcttggc gggctctatg cgcggcttca gcagatggaa
     1201 ggtcccgtag aaacgcaatc gctggaaccc tgatatgcag gaaaagtgat gagagggtgg
     1261 atttgttgtt gacaatcctc tgccgcccgg agaacaattt atcccagtac ttctactact
     1321 ctcagagggg agagagccat ggtcagactt gctggacggc tcagtgtcag cggtttactt
     1381 ctgctcgctg catggggagg cgcgcagacc acatccaccg aagtactggg aaccgtcgtc
     1441 gacgcatccg gcgcggtagt gcccaaggca acagttacgc tgttgcgcgt ggataccggc
     1501 gagaagcgcc aaacgttgac cgacgcatcg ggaaactatt ccttcccgct gattgaaatt
     1561 ggcgcatacc aggtgacggc cgaggtcccc ggcttcaaga cacagacaaa gacaggcatc
     1621 cacgttgagt tgcagcaaaa ggcgcgtgtg aacttcacgc tcgagctggg agcaaccagc
     1681 gagcgggtgg aggtggtggc aagcggcgtg gaactgagga cagacgacgc agcgatcggc
     1741 gccagcatcg accagaagcg ggtagttgaa ttgccgacac taaaccgcaa ctttgcaagc
     1801 ctgctggtgc tcacgcccgg tgtccagtat ggcaaccgca tgggcggcaa cgtgcaggcc
     1861 acaggtgcgt tccccttccc gggcgccgcc actacggtaa gcgccaacgg acagcgcgac
     1921 gccaaccagc gcgtcacgat ggacggcgtg gttgccaccg aaccccttgt taaccaggtg
     1981 atgttcaacc cgtcgatcga tgcgatccag gaagtgaagg tgcagaccgc aacctattcg
     2041 gccgagtacg gacagaataa cggcgccaac gtgcagattg cattaaagag cggcaccaat
     2101 gattttcacg gcactttcta tgacttcctg cgcaacgacc agctagatgc cagagattac
     2161 ttcctgaatt ttcaagttcc ttccggcacg cccgagcgta gaaagaatgc cctgcgccgc
     2221 aaccagttcg gcacctggct cgcaggtcca gtgctgctgc ccggttaccg gggccgcgac
     2281 cgcacgttct ggagtttcaa ttttgaagga acgcgtgaaa cgcaggagag cgtgcagcag
     2341 gctttctggt ttccggaagc gttccggaac ggcgattttt cggcgctgtt gacgccgctg
     2401 attcgagacg ggcggccggt gcgcgggccg attgtcattt tcgatccggt gaccggagaa
     2461 ccgttccgcg actcggctgg acgcatcacg aatatcattc cggccagccg catcaatcgc
     2521 aatgcgcaga acttcatcaa tacctacctg cctctgccgc agttcgttcc ggaggatatc
     2581 ctggacgtca atgtaatcgg aaccgtgcct tctattctgg atcagaatca gtatttcgcc
     2641 cggatcgacc acaatctctc ctcgaaagac cggatcttcg gccgctatgc cacgcaggtt
     2701 tcgagctatg tggcgcagag cctcaacccc aacttcccca ccaactacag tattcgcgcc
     2761 cacaatatcg catctcagta tttgcgcctg ttcagcccca ctgtcctgaa tgaattccgt
     2821 ttcggattca acaaaacgga ccacgaccag agaaacccgc gcacctatac gaatttcgac
     2881 gcggattcgc tcggcgtcgg acggttccgc gtggcagtgg acaacaaccg gaagttcaat
     2941 gaacttgaaa caggcatccc ccctaccggc atcctgcaag gcgacggtgc agcccgcgtt
     3001 gacgaaaacg gcatttacca gatttccgac aatttctctt ttatgcgcgg cagccacagc
     3061 ttcaagacag ggatcgaata tcagcgatac
//
LOCUS       DASTMK010000063         4034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_611725, whole genome shotgun sequence.
ACCESSION   DASTMK010000063 DASTMK010000000
VERSION     DASTMK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611725"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..939
                     /locus_tag="VFQ79_02585"
     CDS             472..939
                     /locus_tag="VFQ79_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU0138563.1"
                     /translation="MIYSRSAEYAIRAFIHLAEVPDGKYAMVKNIAEQADIPAHFLAK
                     ILQQLARKGFLRSSKGPTGGFCLRIPADEITMLQIVDAVDGLTDFHRCPAGMAECNDQ
                     MPCGMHDTWKALRSRIMEYLEGTTIEELAQALDQKRRAVEKRTKSKRPSSRKA"
     gene            977..1588
                     /locus_tag="VFQ79_02590"
     CDS             977..1588
                     /locus_tag="VFQ79_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU0138564.1"
                     /translation="MNSVLVPMVVEQTSRGERAYDIYSRLLKENIIFLGTPIDDNVAN
                     LIIAQLLFLAAEDSERDISLYINSPGGSITAGLAILDTMRLVEPDIVTYCVGQAASMA
                     AVLLASGAKGKRYSLPNSRILIHQPSMSGLAGQAADIDIYAREILRMRESLNQILATA
                     TGQPLEKLARDVDRDYIMEPEQALEYGMIDRIISTRELAPVTK"
     gene            1639..2337
                     /locus_tag="VFQ79_02595"
     CDS             1639..2337
                     /locus_tag="VFQ79_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0138565.1"
                     /translation="MRLLVVEDEKRIAGFLSRGLESAGYAVDVVGDGKSAIEHIQGTD
                     YDLIILDLMLPDTDGLKVLERIRNRKASPPVLILSARGGVDDRVKGLEVGADDYLTKP
                     FAFVELLARVRALLRRGQPLPERLQVGDLVLDCIRRRVSRAGETIDLAPKEFSILEYL
                     MRNRGRPLSRTMIVEHVWDMDYDGLTNIVDVYIRHLRSKIDDRFPLKLIHTVRGIGYM
                     LDSAEQPAEKPAGQ"
     gene            2347..3855
                     /locus_tag="VFQ79_02600"
     CDS             2347..3855
                     /locus_tag="VFQ79_02600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEU0138566.1"
                     /translation="MAFGFFRSLRFRLTAICVVFFSLLLVAAGFVFRAVLEDILGTHV
                     REILEEDWGLVRAYLRIERGKVQWVYDRADPDENYTVERLRRVYLLTDEKGVVQQVST
                     VYQSLGIESPAQIRQAIQSRESIVRLRKTAGGTPFLVRSGVLWDERRKPYYLAIGRSL
                     EERQSTLRQFTLRFFSIFPGLLLVGAVLGWLITGRALRPVNRLADTAQRISASSLDVR
                     IPPRGAGDELDRLIEAFNRMMERISASFQQIRQFSTDVSHELRTPITAIRGQLEVALF
                     AAQNVDQYREAIGNAMEDIERLGQIVKSLLLLSQAESGQLTLEKVRTDLAASIRNLLE
                     QFEIPAGEAGVKVVADLPPVCAAQVDRVQFDRLVSNLVSNAIKYTPRGGTVEIHLRYS
                     PENVVLEVRDTGQGIPKADIPRIFERFYRVRAADSKQEEDSNQEGLGLGLSFVAWIVK
                     AHNGRIDVESTVGEGTRFIVQLPTWADSGGANRELRASESPHDSDKLTLKNK"
     gene            3852..>4034
                     /locus_tag="VFQ79_02605"
     CDS             3852..>4034
                     /locus_tag="VFQ79_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0138567.1"
                     /translation="MSLEIEQREKEGITILDIKGRLTVGETASQLREKLKSMAASGVR
                     SVVLNMKDVDYIDSTGL"
BASE COUNT          851 a         1116 c         1210 g          857 t
ORIGIN      
        1 gcctgaacgc cgtcacaggc aaccaggacg tcgtagcccc atttccggag cgtcgcctcc
       61 aggagatgcc gcgaaatgat cgtatcctca gcgattaaca cgcgcatgac atatctgcca
      121 gctcacccaa agttcatatc ggcagcggga acggtttcat agcacaagtt acagatggtg
      181 aagtactcgt ccgcgtagtc tcttaggagt agtcgtctcc ctgatggcta gatccccaaa
      241 accaaggccg atggagagca atcaagatct ctgtaccggt ttctccccag tttgcgtctc
      301 tttatcaagg gcgagcttac attgcctgct tattttgttc gcggcatagt actctcatcg
      361 gggtgtgggc cggattttac tcttctgcac agtgtttcgc ttgcatgcaa gttgtgaagc
      421 aattaagata aaaacgtatt taacttataa gatcctgccg gggggaatta gatgatttac
      481 tcgcgttccg cggagtatgc gatccgggcg tttatccatc tcgccgaggt gccggacggc
      541 aagtacgcga tggtgaagaa catcgcggag caggctgata ttcccgcgca cttcctcgcc
      601 aagattcttc aacagttggc tcgcaagggt ttcctgcgtt cgagcaaagg gcctactgga
      661 ggattttgtc tgaggatccc tgcggatgaa atcaccatgc tccagattgt ggatgccgtg
      721 gacgggctga ccgactttca ccggtgtccg gcgggcatgg ccgaatgcaa cgaccagatg
      781 ccttgcggaa tgcatgatac ctggaaagcc ctccgttcgc gtatcatgga atatttggag
      841 gggacgacaa tcgaggagtt ggctcaggcc ttggatcaga agcgccgggc cgtcgagaag
      901 aggacgaagt ccaagcgtcc atcctccaga aaagcttaac cggcatcgtt gcctggattg
      961 acggaggagg agtgctatga actcggtatt ggtcccgatg gtcgtcgaac aaacgtcccg
     1021 gggcgagcgg gcctatgaca tctattccag gctgctcaaa gaaaacatca tctttctggg
     1081 cacccctatc gacgataacg tcgccaatct gatcatcgct caactactgt ttttggcggc
     1141 ggaagattcc gagcgggaca tctctctgta catcaactcg cctggcggct cgatcaccgc
     1201 cgggctggcg attttggaca ccatgcggct cgttgagccg gatatcgtca cttactgtgt
     1261 cgggcaagcc gcctctatgg cggctgtgct tctcgcctcc ggcgccaaag gtaaacgtta
     1321 cagcctgcct aactcccgca ttctgatcca ccagccgtcc atgagcggac tagctgggca
     1381 ggctgccgat atcgatattt atgcgagaga gattcttcgc atgcgcgaat cgctgaacca
     1441 gattttggcg acggcgaccg gacaaccgct ggaaaagttg gctcgcgatg tggaccgcga
     1501 ctatatcatg gagccggaac aggcgcttga gtacggtatg atcgaccgta ttatcagcac
     1561 ccgcgaactg gccccggtca cgaagtagcg ctgagcggaa ttcgccaacg cccggcccag
     1621 gcgggcacat cggatcacat gagattacta gttgtcgagg acgagaaacg aatcgccggg
     1681 ttcctgagcc gcggcctgga gagtgccggc tacgccgtcg atgtcgtggg ggacggaaag
     1741 tccgccatcg agcatataca gggcaccgac tacgatctga tcatcctgga cctgatgctt
     1801 ccggatacgg atggactgaa ggtgttggag cgaatccgca accggaaggc cagccctccg
     1861 gtgttgattc tgagtgcccg gggtggcgtg gacgatcgcg tgaaaggtct ggaggtgggc
     1921 gctgacgact acctcaccaa gccttttgcc tttgtcgaat tgttggccag ggtgcgggcg
     1981 ctgctccgcc gcggccaacc cttgccggaa cggcttcagg tgggcgacct ggtgctggat
     2041 tgcatccggc gcagagtctc ccgcgccggt gagacgattg acctggcacc gaaggagttc
     2101 agcatcctcg aatacctgat gcggaaccgc gggcggccgc tcagccggac aatgattgtc
     2161 gagcacgtct gggacatgga ctacgacggg ctcacgaaca tcgttgacgt ctatatccgc
     2221 catctgcgaa gcaaaattga cgaccggttt ccgttgaagc tgattcacac cgttcgcggc
     2281 atcggctata tgctggattc cgcggagcaa cccgccgaaa agcctgccgg gcaataaacg
     2341 gcttcgatgg cattcggatt cttcagaagc cttcggttcc gcctgaccgc tatctgtgtt
     2401 gtcttcttct ccctgctgct ggtggcggcc ggattcgtgt tccgggccgt gctcgaggac
     2461 atcctcggga cgcacgtccg ggagattctg gaggaggact gggggctggt cagggcctac
     2521 ttacgcattg agcgcggaaa agtccagtgg gtgtatgatc gggccgaccc ggacgaaaat
     2581 tacaccgttg aacggctccg gcgcgtctac ctgctaaccg acgaaaaagg cgttgtccag
     2641 caggtctcaa ctgtttatca gtcgctgggg atcgagtctc ccgcccagat ccggcaggca
     2701 attcaatcgc gggagtccat cgtacgcttg cgcaagactg ccggcggaac accctttctc
     2761 gtccggtccg gagttttgtg ggacgagcgg aggaagccct actacctggc gatcggccgc
     2821 tccctggaag agcgccagag cacactccgt cagtttacac tgcggttttt ctccattttc
     2881 cccggcctcc tcctggtggg cgccgtcctc gggtggttga tcacgggccg ggccctccgt
     2941 cctgtgaacc ggttggcgga taccgcccaa cggatctccg catccagcct cgatgtgagg
     3001 attcctccgc gcggagccgg cgatgagttg gaccggctca tcgaagcatt caaccgcatg
     3061 atggagcgga tttcggcatc gttccagcag atcaggcagt tctccaccga cgtatcgcac
     3121 gagcttcgta ccccgatcac tgccattcgg ggtcagttgg aggtggcgct tttcgccgcg
     3181 cagaatgtcg atcaataccg ggaagccata ggtaacgcga tggaagatat cgaacggctg
     3241 ggccaaatcg ttaaatcgct gctgctgctc tcacaggccg aatccggcca gttgacgctc
     3301 gaaaaggtga ggaccgatct ggcggcttcg atccggaatc tgctggagca gtttgaaatt
     3361 cccgccggag aggccggcgt aaaggtggta gcggatttgc ctcccgtttg tgcagcgcag
     3421 gtggaccgcg ttcagtttga ccgcctcgta tcgaacctgg tgtcgaacgc gatcaagtat
     3481 acaccccgcg gcggcaccgt tgaaatccat ctgcgttaca gcccggaaaa tgtcgtgttg
     3541 gaggtccggg acaccgggca ggggattccc aaagccgaca tcccgcgcat tttcgagcgg
     3601 ttctacaggg tgcgcgccgc agattccaag caggaggagg attccaatca ggagggcctg
     3661 ggccttgggc tcagttttgt agcctggatt gtcaaggctc acaacggacg tattgatgtg
     3721 gaaagtactg tcggcgaagg gacccggttt atagtgcaac ttccaacctg ggcggactcc
     3781 ggcggcgcga accgtgaact tcgagcttcg gaaagcccgc acgacagtga taagcttacg
     3841 ttgaaaaaca aatgagcctt gaaatcgagc aacgcgaaaa agaaggtatc actattctgg
     3901 atatcaaggg ccggctcacg gtcggtgaaa ctgccagcca gttgcgtgaa aaactgaaaa
     3961 gcatggcggc gtcgggcgta cggagcgtgg tgctcaacat gaaagacgtg gattacatcg
     4021 acagtaccgg gttg
//
LOCUS       DASTMK010000064         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_4034, whole genome shotgun sequence.
ACCESSION   DASTMK010000064 DASTMK010000000
VERSION     DASTMK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4034"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VFQ79_02610"
     CDS             complement(<1..1565)
                     /locus_tag="VFQ79_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0138568.1"
                     /translation="MNRRRFLQATLSAPAVIASLGSCGMNRDPRPNVLFVISDDLSQP
                     HESAYGSKFVSTPAFDRIAKEGVLFKNAFVSSPSCCPSRGSVLAGLDFFLLKEASMNH
                     TVWPSENLPLYTDMLTEYGYHVGYTGKGWGPGNWKVSGRTVSPAGREFNEMTETPPGK
                     HISRIDYTQNFKAFLEERPEDTPFCFWVGFQEPHRPFDDGIGARNGKNPRSVEVPGFL
                     PDLPEVRRDIADYAFAIEHADRHLARILNLLDERGEMQNTLIIATSDNGMAFPRAKGN
                     LYDYGAHMPLAVRWSARIPGGRVVDDFVSFRDIAPTILEATGLRVPGSMSGESLMYVL
                     DTRQAGQVDPKRRFAVYGIERHFPGSRPDGAGYPSRAIRTSEYLYIRNFTPDRNPVGD
                     IPGPSWPAGDPVGGYGDTDGGPTKTALFEKKDSYPMLFDAAFGKRPAEELYLISEDPY
                     QLKNLAADPQFSDPKRSLSAELDDYLTLHRDPRFVQNGEFFDAVMQRYPVEGSNTSPL
                     VSPATPPAAATPPP"
BASE COUNT          394 a          556 c          568 g          379 t
ORIGIN      
        1 ggaggtggag ttgcggctgc aggcggagtt gcgggcgaca cgagaggcga ggtattcgac
       61 ccttcaactg gatagcgttg catcacggca tcgaaaaact cgccgttctg cacaaaacga
      121 gggtcccgat ggagcgtcag gtagtcatcc agttccgcgc tgagcgagcg cttgggatca
      181 ctaaactgcg gatccgcggc cagattcttg agctgatacg gatcttctga aatcagatac
      241 aactcctccg cgggcctctt gccgaaggcc gcgtcaaaaa gcatggggta agaatccttc
      301 ttctcaaaga gtgcggtctt cgtaggtccg ccgtcggtat ccccatagcc gccaaccggg
      361 tcacccgctg gccaggaggg cccgggaata tcacccacgg gattccggtc tggcgtgaaa
      421 ttgcggatat agagatactc gctggtacga atcgctcgcg atgggtagcc agctccatcg
      481 gggcggctgc ccggaaaatg ccgttcaatg ccgtaaaccg cgaagcggcg cttggggtcc
      541 acctggccgg cctggcgcgt gtcgagcaca tacatcaggc tctccccgct catggaaccc
      601 ggcactcgca atccggtcgc ttcgaggatg gtgggggcga tgtcccggaa gctgacaaaa
      661 tcgtccacca cccgcccacc ggggatacgc gcgctccagc gaaccgccag cggcatgtgt
      721 gccccatagt cgtagaggtt gcccttcgcc cgcgggaatg ccatcccgtt gtccgacgtg
      781 gcgatgatca gcgtattctg catttcgcca cgttcgtcaa gcaaattcag aatccgtgcg
      841 agatgcctat cggcatgctc aatcgcaaag gcataatccg cgatatccct gcggacctcc
      901 ggcagatctg gaagaaatcc aggtacttct acggatcgtg gattcttccc attgcgggct
      961 ccgatcccgt cgtcgaatgg gcgatgcggc tcctgaaagc ccacccaaaa acagaagggc
     1021 gtatcctccg gacgttcttc gaggaatgcc ttgaagttct gagtgtagtc gatgcgactg
     1081 atgtgcttgc caggaggcgt ctccgtcatc tcattgaatt cacgcccagc gggagagacg
     1141 gtgcgtccgg acactttcca gtttcccggt ccccagccct tgccggtata gccgacgtga
     1201 tagccatatt cggtgagcat gtcggtgtaa agcggaaggt tttccgatgg ccacacggtg
     1261 tggttcatcg aagcttcctt aagcaggaag aagtctaagc cggccagcac ggaaccacgc
     1321 gaagggcaac aggaaggcga cgatacaaac gcattcttga acagcacacc ctctttggcg
     1381 attctgtcga aggcgggagt tgaaacaaac ttcgaaccgt aggcgctttc gtgcggttgc
     1441 gataggtcgt cggaaatcac aaaaagcacg tttggacggg ggtcccggtt cattccgcag
     1501 gagcccaggg aagcaatcac ggcgggtgcg ctgagggtgg cttgcagaaa gcgtcggcgg
     1561 ttcatcgccg cctatcgtaa caccatttct ggccgcggag cagccgccag ggggctccct
     1621 ggcgctcgca cggcttccgg gttccggctc ccggcttcca gcgttaagtt ggcagcgctt
     1681 gccttcggcg cgctcgcttg gatgtccctc tcccccgaaa ttccgagtcg ttattgagcg
     1741 tgcgttctca gattaacaag cgcccgtgtc tacgacaaac cgatctatcg gaccgtagag
     1801 gccaccggta tcggtgtcca ggataagagc gctgcgagcc gcgcggccgg gtacttccca
     1861 cagaaagcgg ttagatcgtg atacgccaaa ctcatca
//
LOCUS       DASTMK010000065        22872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_131279, whole genome shotgun sequence.
ACCESSION   DASTMK010000065 DASTMK010000000
VERSION     DASTMK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 22872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22872
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131279"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VFQ79_02615"
     CDS             complement(<1..1788)
                     /locus_tag="VFQ79_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU0138569.1"
                     /translation="MPDGRLRTIAQGRETSIVLALLAIAVLVPTVCVFWFMDQAIQNQ
                     QQVVRQKLMDAHRLQLSLLREKLEGFWQEVGAALEKETSRDTAPEAFARVVATGRVRS
                     AIVLGPGGRVAYPGPPLAPRADLASRNVDWLRAVRLEQDSANLPAAATAYGRIVSNPS
                     VDASLAARAAQGQLRCLVRAGQHEQAVSIVSKVFGNARLANAGDLQGRLISADAELLA
                     LQILRDPESGEFRRIAKRLHARLLDYTNSPMPPDQRVFLMNQMKSLRPGRGFDEFPTL
                     EAETLAARFLAEGGALTHSAALRGSAIPGVLQVSSPKVRLVMLLRTEDALHQMEKLIA
                     SADLSPDIHIAISPPGTRPPKSAESTPIGEALPAWHLSLVIRNESIFEQLARRQNMVY
                     YWIAIVAAITVAGLAALAVGALRRQMRLAGLKANLAATVSHELRTPLSSMRLLVENLL
                     EEPHPDPKKTREYLELIERENVRLSRVIDNFLTFSRLERDHRSFEFSTVSPEAIVREA
                     AQSTRNRFGIPEHEIEVSIEPGLPDVRADENAMVTALLNLLDNAWKYSPAEKRIVLKA
                     YRRGNHVCFEVEDNGIGFSKREAKRIFRSF"
     gene            complement(1802..3232)
                     /locus_tag="VFQ79_02620"
     CDS             complement(1802..3232)
                     /locus_tag="VFQ79_02620"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU0138570.1"
                     /translation="MKMKRYLIHTVLMVLLAGTVPLKAQTAAELLQKGIYTQETLGDV
                     DGAIKIYRQILSTAGEVRTYAAQAQYRLGLCLLRKGERAEAEKTFTTLVEQYPDQKDL
                     VAKAKEQLNVGVALLPVPWPDAELMELDMKLAGGLPIGTAIYSIETDSSKTTPQWSIS
                     SATHVYNGILSLSQVQADRETMKPIASLWRHPMLGEARAQYGDKSVQVELKGKQARDI
                     PVDGPVYDNEEAMWVMRRLPLAPGFKTSLNVLASLTSTCVKIDLQVTGMEDVEVPAGR
                     FRAYKLELNPIKQTFWIASDANRYMVKFEGNGVVAELASVRRPDSSPAQYVDSKLGYT
                     LTAPSRWIPKKHNSPEEMQKTLFLLDPEAAGDVRLWADKATAVDGAIDLVLRKDAEEK
                     VQQRAKALKGYTVRPDSWRVRQVNGQPALSYVADFEEMNRKMTEYLTWVRSPSTSSLF
                     IVRAPETDFEGFRGRIEPMVESLRLK"
     gene            complement(3389..8215)
                     /locus_tag="VFQ79_02625"
     CDS             complement(3389..8215)
                     /locus_tag="VFQ79_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0138571.1"
                     /translation="MSVHQIISGRYEIHNKPVGHGGMGVVYRAYDNVTKRYVALKTMR
                     GIVEPPALDLFTKEWSALAKLSHPNIVDILDTGQFEEEGQQKPFFVMPLLPGVTLDHL
                     IRNASQRLTVERTIEIICQTCRGLQAAHEQGVIHRDLKPSNIFVMEDDAVKIIDFGVV
                     HLTGAQSVTGIKGTLQYMAPEQIAMKPASPASDVFSLGVVCYEALTGRKPFARSTDAE
                     TAEAVRHDIPPPASDINTSVSQILGRVVHKAMAKQPWHRFSSARDFAETLQKALANQP
                     IERFERSRIQPRIERSKRAYAEGDYQFALEILTELEAEGNIDPDMTMLRLQIEQAVRQ
                     KSIRQLLASARTRFEEEEFPLALSKIQEILDIDPENMDALALRGEIEKQRSDRQIENW
                     FRLVNQHIDHLSFSQARKGLQEILAIRANDARALELLAEVDRREQESTRIRQEKEQLY
                     HGALNSYQHGEVSSALTKLERILELNRLSPDFATPERDALYQNLYNQIRSERETARNS
                     YSEGKRYLADRNFAKALEICDDFLRRYPSDPMFQALKLEVEEQQRQERSAYIADVGRR
                     LDAEANLDRRVSILKEAVERYPDEPHLQQSLRLTRERRDLVNSIVSRARQYEERSQFN
                     EALGQLDILRNIYAQYPGLEFEIQRLVRRREDQARHEAKAKWVEQIDRQIELGDFNRA
                     HELARSALEEFNDDRELAGLERIAQQGVERSAEARVLLEQGQRLCLERQFAEGLQALR
                     KASGLNPRDPIIRASLLNALVEQARSVLSHDWRAAEPLVQDALEMDPAHAVAQSLQSL
                     IADSKRQENVNDCISRARELQANNDVEAALFVVENALQAYPSEMRLLQLRSTLRNQLA
                     AATRARSAAPPQPPAQPEPEPVKADTTPPEPVDRKFEEAIFGATPEPVPTRQAPVPEP
                     APAKAKPPAAEPWWRVQWRRIAAWSTELLRGLPAIHKGGLTPLQIGIVIAVPLILIAA
                     LTVAVVGRWSRPGSSIPAPGQHSVILESNIEGTRYFIDGRAVESLPVNLPAGTHRVEA
                     AAEGYRTASESITLEPGGPSPYQFRFDLQPEPLNLRLFSDLKTARVSFDDQPAGELQE
                     GSFVTDGVSYSQHTFRLHEGSGELLTVQFQARPGELPLLSAPVSARNTQAVVVTQLGS
                     RARIYATQPLQAGRTANSLRTIPGDGLELTGLSSSTNTILLTDGKDSRQISVEMSNAP
                     LVSVWLTSDRNIGTVIVESNVPDAQVIVDGRPRTRPLRDGRTVLALEPGRHSIRLSKE
                     DYEDAPEQTVEIGKGDSQRLAFELKPVTRTSALSIEGATPGADVLLDGTRIGRTGADG
                     AFSQADISPGRHTISLRRDNFEERTFQETFTGGATVTISGGSAQLRAFGSVMAKVTPP
                     DATITLKREGDAQSRTIKSGQSISLPAGKYQVSAQSAQHTSRLETIAIEPGKQTAIDW
                     VLAATPAEAKAAPAPKEFTGYFQDPSAWKKYGEWWMRDRPGMAWFALNRGSFTIDFLK
                     QSGRVLFVRTSKRIEWVVDYKDKDNQIAYSLDGKTIQRRQLVAGKTISETRQPAPGCD
                     GDTCRLEIEITPAHVVIRSGSGKFIIDDVKRPDPSAALGRFGFDGEVVLIPRRYQQ"
     gene            complement(8361..9815)
                     /locus_tag="VFQ79_02630"
     CDS             complement(8361..9815)
                     /locus_tag="VFQ79_02630"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138572.1"
                     /translation="MRTVALLFLVIWSGAPAAGQFDLVIAGGRVMDPESGLDAVRHIG
                     IRGGRIEALSESPLSGKDVIEARGSIVAPGFIDLHAHGQTNEANEYQAHDGVTTALEL
                     EIGVPSVAAFLKSREGRALLNFGATASHGAMRAMSMRDIASNADAARDPLQFGYEITA
                     KGRYKPLTHPEEYTQLAAALERGLREGGLGIGMAHQYYPAASQEEIFRVFQMAARWKV
                     PIFVHVRSMSVDAMQEVIANAAATGASLHIVHVNSMSLGALPLVLDMIESARGRGLDI
                     TTEAYPYTAASTFLESTMFDDGWQQRLGISYGDLQWVSTGERLTQETFERYRKQGGFV
                     IMHLMKPEWIQRAVASPFVMIASDGMPYAPGAHPRSAGTFSRVLGLYVREQKALPLME
                     AIAKMTIMPARRLESIAPAMKGKGRIRVHADADITIFDAARVKDTATFGKGLSFSDGI
                     RHVVVNGVAVVRDGKTVAGVYPGKPVTGAYGQRR"
     gene            complement(9849..10523)
                     /locus_tag="VFQ79_02635"
     CDS             complement(9849..10523)
                     /locus_tag="VFQ79_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0138573.1"
                     /translation="MRFVSGLVLLVLSPAWCQVAREANRDYQTPEGRAKVVGILESPS
                     RLANLRPAELVSRLEIRRGSTVVDLGTGTGNLLEDLSAAAGPDGRVIAEDIQQDFLDR
                     ARDRTRKSGLKNVTFVLGTEMDPKLPAGAADLVIVLDAYHHFNYPERMLAAIKRSLHP
                     GGRLAIVEYHKKRGAMEVSPDFALTHIRAGAGQVVEEVKAAGYDLLWQHEHAPGRQYI
                     AMFRMR"
     gene            10619..10999
                     /locus_tag="VFQ79_02640"
     CDS             10619..10999
                     /locus_tag="VFQ79_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138574.1"
                     /translation="MSLKRKSRLISIAGVCFLATLAVPLSFAQTYESARTVVQRTQDD
                     LEQVRRQRPRNDKERERIDNARKSLSDFDRSLSRNKFDKDRLDRAIEDVKNIVEKNTL
                     QARDRDALSTDLADLRKVRSTRGR"
     gene            11105..12574
                     /locus_tag="VFQ79_02645"
     CDS             11105..12574
                     /locus_tag="VFQ79_02645"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU0138575.1"
                     /translation="MKDRLTTSAGAPVADNQNSLTAGSRGPVLLQDYQLLEKLAHQNR
                     ERIPERVVHAKGWGAFGTLTVTNDITRYTRAKLFSQIGKTTELLIRFSTVAGELGAAD
                     AERDVRGFAMKYYTEEGNWDLVGNNTPVFFVRDPLKFPDFIHTQKRHPRTNLRSATAM
                     WDFWSLSPESLHQVTILMSDRGIPVSPRFMNGYGSHTYSFWNEAGERFWVKFHFKTQQ
                     GHRHLTNEEAAEVIGRTRESYQEDLFGAIEAGDFPRWKVFVQIMPELDAEKTWYNPFD
                     LTKVWPHADYPLIEVGMMELNRNPRNYFADIEQAAFSPSNVVPGIGFSPDKMLQARIF
                     SYADAHRYRLGAHYEALPVNAPKCPVRHYHKDGPMRFFQNDTGNPDAYYEPNSFQGPA
                     QDPSVAEPPLRISGDASRYDHREGNDDFSQPRALFRLFDEGQRERLFSNIADAMDGVP
                     EAIVERQCALFDQVDGDYGAGVRAALARKKAKSSSAKAG"
     gene            12651..13112
                     /locus_tag="VFQ79_02650"
     CDS             12651..13112
                     /locus_tag="VFQ79_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138576.1"
                     /translation="MNNPTAIQASAEHGAAIVALEAEHDVIDLELGTLKSAILSGAEG
                     ALIIYVLDRSIAFCEEHFTHEEDFLRDHGYGRVDDHAALHQRLLKSFRCARDLATGDS
                     LPLAALDAFELLQEFKEHVERYDRPAYMEVEKRLPQKFKTNGAFAAHTAIR"
     gene            complement(13069..13935)
                     /gene="corA"
                     /locus_tag="VFQ79_02655"
     CDS             complement(13069..13935)
                     /gene="corA"
                     /locus_tag="VFQ79_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEU0138577.1"
                     /translation="MAWHDIRDPGDPELDRLAAQYQLHPLHIEDCRHRNQNAKIEEGP
                     GYLFVVLKPVLLNQDQSLDVFDLDLFIGKDFVITVEEGDCPSAREILNQVRGQEARLR
                     PDQAFYRVMDGIVDTYAPVLDGLNDEIDRLEDEVLESPQPRTLQKVLSTKRCLITIRR
                     VMANTRDVTAHLQRSGTELVDPQLWPFFRDVYDHIARNLDTIDMQRDLLTGSMDIYLS
                     SVANRTNQVMKVLTVLGTAALPALVISGFYGMNLHHLPWADSPYALAVVAALMVLTTS
                     GLLYALRKLHWF"
     gene            14022..14549
                     /locus_tag="VFQ79_02660"
     CDS             14022..14549
                     /locus_tag="VFQ79_02660"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU0138578.1"
                     /translation="MILKLKRTPGIYLVGFMGSGKSTVGRLLADRLGWNFIDVDEQIE
                     AEQGVPIFEIFEKSGEQEFRRIEREAIERRVGMVEAGHPMVLALGGGAFVEPHNRDLL
                     KENGITIWLDCPFATVKARVARAAHRPLARDPKRFEDLYHNRRAAYEQADYRIEIDGD
                     DPEQPVRSILALPIF"
     gene            14773..16104
                     /locus_tag="VFQ79_02665"
     CDS             14773..16104
                     /locus_tag="VFQ79_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU0138579.1"
                     /translation="MTESTLRKHALQIFQAALKAADPVEAVRRHVRVEDDVLLVDGRK
                     YRLKNFEKIFVVGAGKAGAAMAKAVETLLGRRISGGFINVKDGHTLPLRRIELNEASH
                     PVPDERGVRGAERIAEIASRAGEKDLVLCLISGGASALMPAPAAGLTLEEKRQTTKLL
                     LNSGASIHEINSVRKHLSLLKGGQLARLASPATVLSLLLSDVIGDDLDVIGSGPAAPD
                     SSTFADARDVLEKYGIYSHVPPAVKQRIEDGISGVVEDTPKSGDPIFERTRNVVVGSN
                     RLAVDAAVKRARELNLKPLVLSTFIEGETREIARMHAAVAKEILTSGRPLKPSCCLIS
                     GGETTVTIRGEGLGGRNQEFVLAAAMDIAGLRNVVVLSGGTDGTDGPTDAAGAIADGN
                     TIARGDKAGMSASNALSFNDSYHFFEPLGDLIKTGPTNTNVMDIRLVLVAG"
     gene            16108..16458
                     /locus_tag="VFQ79_02670"
     CDS             16108..16458
                     /locus_tag="VFQ79_02670"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0138580.1"
                     /translation="MSFWRSELIDFALRQETEKHICEQMEWIARDPENATPYYNLAQL
                     YRIEGRAEEALGLLLEAVRLDAEFAGAHVALAGMYAVKGDYPAAWKHARSAESCGNSA
                     GIELLSRYGVATDR"
     gene            complement(16445..17884)
                     /locus_tag="VFQ79_02675"
     CDS             complement(16445..17884)
                     /locus_tag="VFQ79_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU0138581.1"
                     /translation="MRPRSIAGPMVLILIGVAFLLYNLRPDLRIIDLLSRFWPYLLVG
                     WGVLRLFEIVIWAARSRPLPTSGVSGGEWTLVVFLVLIGSGARFVHERGDWWWPRARI
                     MARGIEVFGEPYDYPLAERSVPAPKNARVIIENLRGNTRIIGADIAEVKIAGRKTVRA
                     LQRSEADADDKRTPLEATAQGEQVLIRTMQERASGENRVSTDLEITVPRGASIEAKGR
                     RGDFDITDLDGGVEIKSDNAGVRLANIGGAIRIETRASDIIRASGAGASVELKGRGHD
                     LELDTVAGPITITASYYGDVQLRNLKQSLRWSAGQNEFNLEQLSGELRMDLREVSGSN
                     LVGPVKISARSRDIDLSNFTGALEISVDRGDIHLNPVKLPLSPMNVRSHSGNIEVALP
                     PKAEFTVKAVSRRGDIENDFGPPLERTQNGRSFELAGAVGKGPLLNLASDRGTVSVGK
                     SSAFEQSNPPLPPKAPKPPRATSLTVIDQ"
     gene            complement(17881..18099)
                     /locus_tag="VFQ79_02680"
     CDS             complement(17881..18099)
                     /locus_tag="VFQ79_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138582.1"
                     /translation="MNNSRLAFVQAIRGPILLIALGVLFIVDHAGGPPFRQTWPVLII
                     LFGLLKLLERAVIRQPAPVPSSPPERLS"
     gene            complement(18086..18769)
                     /locus_tag="VFQ79_02685"
     CDS             complement(18086..18769)
                     /locus_tag="VFQ79_02685"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HEU0138583.1"
                     /translation="MNCVNHPELAAAAYCRSCGKALCQNCARTAQGTIYCEEHVPAQP
                     ALAPPSDSPWTAPAPAPGSPDPGASPGLAFLLGLIPGVGAIYNGQYAKGLIHVIILGF
                     LISILSSGAAGGLEPVFGLVTAVWYFYMPFEAYHTARKRQLGQPVDEFSSLVPMRGTH
                     TNSPVAPVVLIVLGVLFLLNNLDLLNFYYVLRYWPVFLIALGGYMLYVRFKDSEGEAV
                     RREASNEQQ"
     gene            complement(18766..19401)
                     /locus_tag="VFQ79_02690"
     CDS             complement(18766..19401)
                     /locus_tag="VFQ79_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0138584.1"
                     /translation="MKCAEFETLLCDYVDGTLAPDRKSGAEQHLGECEACAGLARDCS
                     LAVTFIGRASAVEPPHELMTRIAFSIPAGLRRRGGAMARFHRIFGPILQPRFAMGMAM
                     TILSFSMLSQFLNIPIRQLTVDDLRPEKVWVVVEDRVHRVWDRGVKYYESMRLFVEIQ
                     SRLKDLTATDPAVQAPSATPGSNSPAGATGAGDTSGASGSSEPAPKGRSNQ"
     gene            complement(19398..20072)
                     /locus_tag="VFQ79_02695"
     CDS             complement(19398..20072)
                     /locus_tag="VFQ79_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0138585.1"
                     /translation="MAAFTAFAQMSTADSSSVRWTEETAGRGCLQLPGKPLDLDSNLV
                     ERCLAGDESAWEDLVKIHTRRIYGICYRFTGSDGEAQDLTQEVFLRVFRSIGSFRAGE
                     GSFVVWLTRLTRNLLIDHYRRSRMDRASDSIEDQLAVLEVSSTPSTRPDGALAGREAS
                     ELLQQALQKLSPELRETVILRDIEELEYKEIAGILRVPEGTVKSRLNRGRAELARVLR
                     RQRVVV"
     gene            20340..21353
                     /locus_tag="VFQ79_02700"
     CDS             20340..21353
                     /locus_tag="VFQ79_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU0138586.1"
                     /translation="MIAIGVVCVAAVVLALVRLRGFDWDKFFAAFRNLNWGWLTASAL
                     MALGTYVVRAERWRVMIRPICPRASFWGLLVSTAIGFTALVLFGRPGEFVRPYLIATK
                     NKLTITSQVAVWFLERIYDLLIVLVLFGFALTRVQASGASLGPDMRWLLEIGGHIVGI
                     AGTICLVVLIFLRYFSETFRRRITDALGVLPARLEERAVKTVDALFEGVQSARSGTFV
                     LQLVFYSIAEWIVITACFLFLFWSFPDTAAFTLTDVLIFVGFVAIGSVVQIPGIGGGM
                     QVASVIVLSEVFGIGFESASALAILLWAITFLVIVPIGLLLAFHEGLNLRKLRDIEQK
                     SEI"
     gene            21350..21823
                     /gene="nrdR"
                     /locus_tag="VFQ79_02705"
     CDS             21350..21823
                     /gene="nrdR"
                     /locus_tag="VFQ79_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU0138587.1"
                     /translation="MICPFCGFRQDRVIDSRESKEGDVIRRRRQCLECERRFTTYERI
                     DEIPYMVVKKDGRREKFDRQKVLGGLLRACEKRPVPMGKLAEVVDEVEGRLAETQDRE
                     LPTTIIGEILMERLRTLDKIAYVRFASVYRDFQDVEAFLNELKNLIVHKREKRPL"
     gene            22059..>22872
                     /locus_tag="VFQ79_02710"
     CDS             22059..>22872
                     /locus_tag="VFQ79_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEU0138588.1"
                     /translation="MDHFEDQYIGDSHEPLGLTFDEEPNFFHPEEAHDEEFPAAHQVE
                     ESIYTDDPVRVYLREMGAVPLLTREGEVDLAHKMERGKLRMQKAISRSALVQQMVVEY
                     AEQLKRGDIDLEDLVDLGDVEEGSAAEAKRRSDVRQQFADVVLLCRRKQQLTEKLNAA
                     NGNNKKIRKRASGKLNRCRVETSRTIRAIPFNAHRWREFAKEIERTVDDVVQLEAEQR
                     KLEEKGNPALQARIREIRREIRKREAVAGASVPDLKHSLSVIRHGEQEAERAK"
BASE COUNT         4536 a         6650 c         6985 g         4701 t
ORIGIN      
        1 gaagctccgg aaaatccgtt tcgcttcgcg tttgctgaaa ccaatgccgt tgtcttcgac
       61 ttcgaagcaa acgtgattcc cacgccggta cgccttcaga acgatgcgct tttcggccgg
      121 agagtatttc caggcgttgt ccagcagatt gagcagagcg gtgaccatgg cattctcatc
      181 cgcccggaca tccggcaagc cgggttcgat ggagacttcg atttcgtgct ccgggatgcc
      241 gaaacggttg cgtgtggact gcgcggcttc tctgacaatg gcttccgggg agacggttga
      301 gaactcaaag gaccggtggt cgcgttcgag acgcgagaag gtgaggaaat tgtcgattac
      361 acggctgagc cgcacgttct cacgctcgat caactccagg tattcacggg ttttcttcgg
      421 gtctggatgc ggctcttcga gcaggttctc caccagcagg cgcatggaag acagcggggt
      481 tctaagctca tgtgagaccg tggcggcgag gttggctttg aggcctgcga gacgcatctg
      541 gcggcgcaac gcgcccaccg ccagcgccgc gagccccgcc accgtaatag cggcgacaat
      601 agcgatccag taatacacca tgttttgccg gcgcgcgagc tgttcaaaaa tcgattcatt
      661 gcgaatcacg agggagagat gccacgccgg cagcgcctcg cctatgggag tggattccgc
      721 cgattttggc ggccgggtcc cagggggaga gatcgcgatg tgtatgtcgg gcgacagatc
      781 cgccgaggcg attagcttct ccatttggtg cagggcatct tccgtccgca gcagcatcac
      841 cagccgtacc ttcggggagc tcacctgcaa gacgcccggg attgcgctgc ctcgtagcgc
      901 tgcactgtgc gtcaatgctc ctccttcggc aagaaaccgg gcggcgagcg tctcggcttc
      961 cagtgttggg aactcgtcga atcctctacc gggcctcaac gatttcatct ggttcatcag
     1021 aaatactcgc tggtcgggcg gcatcgggga attcgtgtaa tcgagcaggc gggcatggag
     1081 ccgtttggca atccggcgaa actcgccgct ttcagggtcc cgaagaatct gaagcgcaag
     1141 caactcggcg tcggcggaga tcagccggcc ttgcaggtcc cctgcattcg cgaggcgcgc
     1201 gttgccgaac accttcgaca caatcgaaac cgcttgctca tgctgtcctg cacgcaccag
     1261 gcaccgtagc tgcccttgcg ccgcgcgagc agccaggctt gcatccaccg atggattgga
     1321 gacgattcta ccgtaggcgg ttgcagcggc gggcaggttc gcggagtcct gttccagccg
     1381 cacggcccgg agccagtcta cattccggga cgcgaggtcc gcgcgcggtg caagcggcgg
     1441 gcccgggtag gccacccggc ctcccggacc caatacaatc gcgcttcgaa cgcgaccggt
     1501 tgcgacaact cgggcgaaag cctccggcgc ggtatccctg ctggtctctt tctccagggc
     1561 ggcgcccacc tcctgccaaa agccttccaa cttttcgcgg aggagcgaga gttgcagccg
     1621 gtgtgcatcc atcagctttt gccggactac ctgttgctgg ttttggatcg cctggtccat
     1681 gaaccagaac acgcaaactg tagggaccag gaccgctatc gcgagcagcg cgagaacgat
     1741 cgaagtctcc cgcccctggg cgatggtgcg aaggcgtccg tccggcacaa tacaggcgct
     1801 cctacttcag gcgcaggctc tcgaccatag gctcaatgcg cccgcgaaag ccctcgaaat
     1861 cggtctcggg ggcgcgcacg atgaacaatg agcttgtaga gggactgcga acccaggtga
     1921 gatattcggt catcttacga ttcatttcct cgaagtccgc gacataggat aaggccggtt
     1981 gtccattgac ctgccgcacg cgccaactat cgggacggac ggtatatccc ttcaatgcct
     2041 tcgcccgttg ctggaccttt tcttccgcgt ccttgcgcag gaccaagtcg attgcgccgt
     2101 ccacggcagt cgccttgtcc gcccacaggc gaacatcgcc cgccgcctcg ggatcgagca
     2161 ggaacaacgt cttctgcatc tcttccggcg aattgtgctt tttgggaatc catcgtgagg
     2221 gggctgtgag ggtatagccg agtttcgaat ccacgtactg tgccgggctg ctatccggcc
     2281 gccggaccga cgccagttcc gcaaccacac cattcccttc gaacttgacc atgtaacggt
     2341 tggcgtccga cgcaatccag aaggtctgct ttatcggatt caattccagc ttgtaagcac
     2401 ggaagcgccc cgccggtact tcgacgtctt ccatccctgt tacctgaaga tcgatcttca
     2461 cgcaggtgga cgtgagcgaa gccaacacat taaggctggt cttgaacccg ggcgcaagtg
     2521 ggagccggcg catcacccac atcgcttctt cgttatcata gaccgggccg tcgaccggaa
     2581 tatcgcgtgc ctgtttccct ttcagttcca cctgaacgct cttgtcgccg tattgtgcgc
     2641 gggcctcgcc cagcatcgga tgccgccaca ggctggcgat gggcttcatc gtttcgcggt
     2701 cggcctgaac ctggctcagg gaaagaatgc cgttgtatac gtgtgtcgca ctggagatag
     2761 accactgcgg cgtggtcttg gaggaatctg tttcgatgga atagatggct gttccgatcg
     2821 ggagccctcc ggccagtttc atgtcaagct ccatcaactc ggcatccggc cacggtacgg
     2881 gaagcaaggc gactcccacg ttcagttgtt ctttcgcctt tgccacaagg tctttctggt
     2941 cggggtactg ctcgaccaag gtggtgaacg tcttttcggc ctccgcacgc tctcctttgc
     3001 gaagcagaca taggccgagc cggtactgcg cttgcgctgc ataggtgcgc acttcgccgg
     3061 cggtggacag gatttgacgg tagatcttaa tagctccgtc cacatcgccc agcgtttcct
     3121 gggtgtagat tcccttttgc agcaattcgg cggctgtctg ggccttcagc ggaactgtgc
     3181 ccgcaagaag aaccatcaaa accgtatgga tcaaataccg cttcatcttc atggctccat
     3241 cctttcctgg gtgagccaag cgcctgactc tgatcgagtc tacaaagcag atgttaccgg
     3301 attattacct cgttgtccta ttttgtctga ccgcttgctc atgcgcgcgg ctccgttccg
     3361 ggccacgacg gtcagggagt ggtaataact attgctggta acgcctgggg atcaacacca
     3421 cttcgccgtc aaatccgaat ctgccgaggg ctgccgaggg atcggggcgt tttacatcgt
     3481 cgatgataaa ttttccggaa ccgctccgta tgacgacgtg cgccggtgtg atttcaatct
     3541 ccaggcggca tgtatcgcca tcgcatccag gcgcgggctg gcgggtttcc gagatcgtct
     3601 ttcccgccac aagttgcctg cgctggatgg ttttgccatc caggctgtat gcgatctggt
     3661 tgtctttatc tttgtagtca actacccatt cgatgcgttt cgatgtgcgc acgaacagaa
     3721 cacggccgct ttgtttcaga aaatcgatcg taaaggagcc gcggttgaga gcgaaccaag
     3781 ccatgccggg acgatcccgc atccaccatt ctccgtactt cttccacgct gacgggtcct
     3841 ggaagtaccc ggtgaattcc ttcggtgccg gtgctgcctt tgcttccgcg ggagttgctg
     3901 ctaataccca atcgatggcc gtctgtttgc cgggttcgat agcaatcgtt tccagacggg
     3961 acgtgtgttg cgcggattga gcgcttacct gatattttcc cgcgggcaac gagatgctct
     4021 ggccgctctt gatcgtacga gattgcgcat cgccttcccg cttcaaggtg atcgtagcgt
     4081 ccgggggagt aaccttggcc atcaccgaac cgaacgccct caactgagcc gagccgccgg
     4141 aaatcgtgac cgtcgcgccg cctgtaaacg tttcctgaaa ggtgcgctct tcgaaattgt
     4201 ctctgcgcag cgaaatcgta tgacgtccgg gcgaaatatc ggcttgtgaa aatgcgccgt
     4261 cggcgccggt cctgccgatc cgggtgccgt ccagcagaac atccgcaccg ggtgttgctc
     4321 cctcgatgga cagtgccgaa gtgcgggtaa ccggtttgag ctcgaatgcc agcctctggc
     4381 tgtcgccttt cccgatttcg acggtttgtt ccggtgcgtc ttcgtaatcc tctttcgaca
     4441 gccggataga atgcctgccg ggttcgagtg ccagtaccgt ccttccatct ctcaacggcc
     4501 gcgtccgggg acgcccgtcg acaatcactt gtgcgtcggg cacattcgat tcaacgataa
     4561 cggttccgat atttcgatcc gacgtgagcc agacacttac caggggcgca ttggacatct
     4621 cgaccgaaat ctggcgggag tcctttccat cggtcagcag gatggtattg gtggaggagc
     4681 taagcccggt cagttccagg ccatcgccgg gaatggtccg gaggctgttg gccgtccggc
     4741 ctgcctgaag aggttgcgtc gcgtagattc tcgctcgcga gcccagttgc gtgaccacca
     4801 cagcctgtgt gttccttgcg gagaccggcg ccgacagcag gggaagctcg ccgggccgcg
     4861 cctggaattg caccgtgaga agttcaccgg aaccctcatg caacctgaac gtgtgctggg
     4921 agtacgacac accatcggtg acgaaactgc cctcctgaag ttcgcccgcc ggctggtcgt
     4981 caaaagaaac gcgtgcggtc ttcaagtcgg aaaaaagacg gaggttcagc ggctccggct
     5041 ggaggtcgaa gcggaactgg taggggctcg gtcctccagg ttccaacgta atagactcgg
     5101 atgctgttcg atagccctcg gcggctgctt ccacacggtg agtgcccgca ggcagattca
     5161 ccggcagcga ttcgaccgcg cggccatcga tgaaatacct ggttccctcg atattcgatt
     5221 cgaggataac ggagtgctgg ccgggcgccg gaatggagga gcctggccgc gaccagcgtc
     5281 caaccaccgc aaccgtcagg gcggcaataa ggataagcgg aacggctatc actatgccga
     5341 tttgaagagg cgtaaggccg cccttgtgaa tggcgggcag cccgcggagc agctccgtgc
     5401 tccaggctgc gatgcgccgc cattgaacgc gccaccatgg ctcggcggca ggcggttttg
     5461 cttttgccgg ggccggttcc gggacaggcg cctggcgcgt tggaaccggc tccggcgttg
     5521 ctccgaagat cgcttcttca aacttacggt ctaccggctc cggcggggtg gtatccgctt
     5581 ttaccggttc gggttccggc tgcgcgggag gctggggcgg cgcggcggat ctggcccgtg
     5641 tggccgcggc gagttggttg cgcagcgtag accggagctg gagcaagcgc atttcgctcg
     5701 gataggcctg tagtgcgttc tccaccacga acaaggccgc ttccacgtca ttgttcgcct
     5761 gtagttcccg agctcgtgag atacagtcgt tgacgttctc ctggcgtttg gagtctgcga
     5821 tgagcgattg gagactttgc gccacggcgt gggccgggtc catttcgagc gcgtcctgaa
     5881 cgaggggctc tgcggctcgc caatcgtggc tcagaacgga acgtgcctgc tccaccagag
     5941 cattcagcag ggaggcgcga ataatggggt cgcgcggatt gagcccggac gcctttctga
     6001 gggcctgcag tccttccgca aactggcgtt cgagacaaag cctctgcccc tgctccagca
     6061 ggacgcgcgc ctcggcgctg cgctcgacgc cctgctgcgc tatccgttcc agtccggcca
     6121 actcacggtc atcgttgaac tcttcgagtg cgctgcgggc aagctcgtgt gcgcggttaa
     6181 aatcgcccag ctcgatctgg cggtcgatct gttctaccca tttcgctttc gcttcatggc
     6241 gggcctgatc ttcgcggcgg cgcaccagcc gctggatttc gaactcgagt cccggatact
     6301 gggcatagat gttccgcagg atgtccaatt gcccgagcgc ttcgttgaac tggctgcgct
     6361 cttcgtattg cctggcacgg gaaacgatcg agttcaccag gtccctgcgc tcccgggtga
     6421 gccgcaagga ctgctgtaga tgaggctcgt cgggataccg ctccaccgct tctttgagga
     6481 tgctcacgcg gcggtcgagg ttggcctcgg catcgaggcg gcgtcccacg tctgcgatgt
     6541 aggcggagcg ctcctgcctc tgctgctctt cgacttcgag ctttagcgcc tgaaacatgg
     6601 ggtcgcttgg ataccggcgc agaaagtcat cgcaaatctc tagggctttc gcgaaattcc
     6661 ggtccgcgag gtagcgtttg ccttctgagt aggaattcct tgcggtttcg cgctcagacc
     6721 ggatctggtt atacaggttc tggtaaagag cgtcgcgttc cggcgtcgcg aaatcggggg
     6781 agagccggtt caactccaaa atccgctcga gcttggtcag cgccgaactg acctcgccgt
     6841 gctggtagga gttgagcgcg ccatggtaca actgctcctt ctcctggcgg atccgtgtgg
     6901 attcctgctc gcgccggtct acttccgcca gcaattcgag ggcgcgcgcg tcgttcgcgc
     6961 gaatggccag aatctcctgc aaccccttcc gcgcctggct gaaggagaga tgatcgatgt
     7021 gctggttcac aagccggaac cagttttcaa tctggcggtc gctccgctgc ttctcgattt
     7081 cgccgcgcag cgccagggca tccatgttct cgggatcgat atccaggatc tcctggatct
     7141 tggaaagagc gagcggaaat tcctcctctt cgaagcgcgt tcgcgcgctc gccaaaagct
     7201 gacggatcga cttctggcga acggcctgct caatttgcag gcgcagcatc gtcatgtccg
     7261 gatcgatgtt tccctcggct tcgagctccg taagaatttc gagcgcgaac tggtagtcgc
     7321 cctcggcata cgcgcgtttc gatcgttcga tccgcggctg aatgcgcgac cgctcgaacc
     7381 tctcgatggg ctgattggcc agcgctttct gaagagtttc cgcaaagtcc cgggcgctcg
     7441 agaaacggtg ccacggctgc ttggccatcg ctttgtgcac gacacggccg aggatctggc
     7501 tgacagacgt gttgatatcg gaggccggcg ggggaatatc gtggcgcacc gcttccgccg
     7561 tctcggcgtc cgtgctgcgg gcaaatggtt tgcgaccggt caaggcttcg taacagacca
     7621 cgcccagcga gaaaacgtcc gaggcgggcg aagcgggctt catcgcaatc tgttccggcg
     7681 ccatgtactg cagcgtgcct ttgatgccgg taaccgattg agcgccggta agatggacca
     7741 cgccaaagtc gatgatctta acggcatcgt cttccattac gaagatgttg ctgggcttaa
     7801 ggtccctgtg gatcacgccc tgttcgtggg ccgcctgcag cccccggcag gtttggcaga
     7861 tgatctcgat ggtccgctcg acggtcaggc gctggctggc gtttcgaatg aggtggtcca
     7921 gcgtgacgcc gggcagcaaa ggcataacga agaagggttt ctgttgtccc tcctcttcga
     7981 attggccggt atcgaggatg tccacgatgt tggggtggct cagcttggcc agggcactcc
     8041 actctttggt gaagaggtcg agagcgggcg gttccacgat gccgcgcatg gttttgagtg
     8101 ccacgtaccg cttggtgaca ttgtcgtagg cgcggtagac cacacccatg cctccgtgtc
     8161 ccaccggttt gttgtggatc tcgtatctgc ccgagatgat ctgatggaca gacatggggt
     8221 tcggggacat taggatacca gggaacttcc tctaatatca aaggcggatt tacgctcaga
     8281 aaagcaccaa aaacatccga ggcggccggc ggttgggtaa aaaggcgcta ggttattcaa
     8341 atgattgaag atttccggtt ttaacgacgt tgcccgtaag cgcccgtcac cggcttgcca
     8401 gggtatacgc ccgccacggt tttgccatcg cgcacgaccg ctacgccgtt caccaccaca
     8461 tgccgaatgc catctgagaa gctgagcccc ttgccaaagg tggcagtatc cttcacgcgg
     8521 gcggcgtcaa aaatcgtgat gtccgcatcg gcgtggaccc ggatccggcc tttacccttc
     8581 atcgcaggcg caatcgattc caggcgccgt gcgggcataa tcgtcatttt cgcaatggct
     8641 tccattaacg gaagggcttt ctgctcccgc acatagaggc caagcacgcg agaaaaggtg
     8701 ccggcgctcc gcggatgcgc gcccggagca tagggcatgc catcggaggc gatcatcacg
     8761 aatggcgatg ccacggcgcg ttgaatccac tccggcttca tcagatgcat aattacgaag
     8821 ccgccctgct tccggtagcg ctcgaaggtc tcctgagtaa ggcgctcccc ggtagagacc
     8881 cattgcagat cgccgtagga gattccgagg cgctgctgcc agccatcgtc gaacatcgtg
     8941 gattcgagga acgtggacgc cgccgtgtag ggataagctt ccgtggtgat gtcaagcccg
     9001 cggccgcgcg cggattcgat catgtccaac accagcggca gcgctcctag actcatgctg
     9061 tttacgtgaa cgatgtgcag cgaagcgccg gtagcagcgg cattggcaat tacttcctgc
     9121 atggcgtcca cgctcatcga gcgcacgtgg acgaaaatcg gaaccttcca cctcgcggcc
     9181 atctgaaaca cccggaaaat ctcttcctgg gacgccgccg gatagtattg atgcgccatg
     9241 ccgatcccga gcccgccctc gcgcaggcca cgctccaaag cggccgccag ttgcgtgtac
     9301 tcttccggat gggtgagtgg cttatagcgt cctttggccg tgatttcgta gccaaattgc
     9361 agcgggtccc gtgccgcgtc cgcattcgat gcaatatcgc gcatcgacat tgcgcgcata
     9421 gcgccgtggg acgctgttgc tccgaaattc agcagtgccc ggccctcgcg ggatttcaaa
     9481 aaggccgcta ccgaggggac gccgatctca agttccagag ccgtggttac gccatcgtgt
     9541 gcctgatact cgttggcctc attcgtctgg ccgtgcgcgt gaagatcgat gaaacccggc
     9601 gcgacaatgc ttccgcgggc ctcgatcacg tcttttccgg acagcgggct ttccgagagg
     9661 gcctcgatac ggccgccgcg gatgccgatg tgccgcacgg catcgagtcc gctctccggg
     9721 tccatcacac ggcctccggc gatgaccaga tcgaactgcc cggctgccgg tgcaccggac
     9781 cagatcacaa ggaacaacaa tgcaacggtt ctcactactt cctccacgaa cgtcagtgat
     9841 tgtgtatgtc agcgcatgcg gaacatggcg atgtattgcc gccccggcgc atgctcgtgc
     9901 tgccagagta agtcgtaccc cgcggctttg acttcctcga cgacctgccc ggcgccggcg
     9961 cggatatgcg tgagtgcgaa gtccggagaa acctccatcg caccccgctt cttgtgatac
    10021 tccacgatcg ccaggcggcc gcccgggtgc aggctccgtt tgatcgcggc cagcatccgt
    10081 tcgggatagt tgaagtgatg ataggcgtcc agcacgatga cgagatcggc ggcgcctgcc
    10141 ggcagcttgg gatccatttc cgtgcccagc acaaacgtca cgttcttcaa cccggacttg
    10201 cgtgtgcgat cccgggcgcg gtcgagaaaa tcctgctgta tgtcctcggc tatgacgcgt
    10261 ccatcgggtc cggctgccgc gctcaggtcc tccagcaggt tgcccgtccc ggttccgaga
    10321 tccactaccg ttgaaccgcg gcggatctcc agccgcgaaa cgagctccgc cggccgaaga
    10381 tttgccaaac gggaaggcga ttccagaatt cccacaacct tggcgcgccc ctcaggcgtc
    10441 tggtaatccc gattggcttc gcgagcgacc tggcaccagg ccggagataa cacaagtagc
    10501 accaggccgg aaacgaatct catacagccc tccgttgttg agtagattgt ctctcaagcc
    10561 ggcagaggcc ggcaacggcg gacggataca aaacctggcg ctgcggcgtc ttttggtcat
    10621 gagtttaaaa cgaaagagcc gtctcatctc aattgccgga gtatgcttcc tggcaacgct
    10681 ggccgttccg ttgtcattcg cccagaccta cgaatccgcc aggaccgttg tacaacgcac
    10741 ccaggacgac ctggagcaag tccggcgaca aaggccgcgc aacgacaaag aacgggagcg
    10801 gatcgataat gcgcgcaaat cgctatccga cttcgaccgc tccttgagca ggaacaagtt
    10861 cgataaagac cgcctggatc gagccatcga agatgtcaaa aatatcgtgg agaaaaacac
    10921 tcttcaggcg cgggaccggg atgcattgag caccgacctc gccgacctgc gcaaagtccg
    10981 gtccactcgc ggccggtgaa cggaaccatt ccgttcgatc ggcgctcttt ccgccggaca
    11041 accgggtgta cggggccacc cgggtgaagg tacactggtg gacaagatat taggagcaca
    11101 aactatgaaa gatcgcttga ctaccagtgc tggcgccccg gtcgccgata atcaaaacag
    11161 cctcaccgcg ggaagccgtg gccccgtttt attgcaggac tatcaactac tcgaaaaact
    11221 ggcccatcag aaccgcgagc gtattccgga gcgtgtggtc catgccaagg gctggggagc
    11281 atttggcacc ctcaccgtca ccaacgatat cacgcgttac acgcgggcaa agctctttag
    11341 ccagatcggc aagacgacgg agcttctaat ccgtttctct accgtcgccg gcgagttggg
    11401 cgcagccgac gccgagcgcg atgtgcgcgg ttttgccatg aagtattaca ccgaggaagg
    11461 gaactgggat ctggttggca acaacacccc ggtgtttttc gttcgcgatc ccttgaagtt
    11521 ccccgatttc atccataccc agaagcggca tccccggacg aatctgcgca gcgcgaccgc
    11581 aatgtgggat ttctggtcgt tgagccccga gagccttcac caggtgacga tcctgatgtc
    11641 cgatcgcgga atccccgtga gcccgcgatt catgaatggc tacggcagcc atacgtacag
    11701 cttctggaac gaagcgggtg aaagattctg ggtcaagttc cacttcaaga cgcaacaggg
    11761 gcaccgccac ctgacgaacg aagaggcggc ggaggtaatc ggccgcacgc gggaaagcta
    11821 tcaggaggac ctgttcggag ccatcgaagc cggagacttc ccccggtgga aggtgtttgt
    11881 tcagatcatg ccggagctgg atgcggagaa aacgtggtac aacccgttcg atctcaccaa
    11941 ggtttggccg cacgccgact accccctgat cgaggtcgga atgatggagc tcaatcgaaa
    12001 tccccgcaac tattttgcag atatcgaaca ggctgctttc agcccgtcca acgttgtgcc
    12061 cggcatcggc ttcagcccgg acaaaatgct gcaagcccgc atcttttcct acgccgacgc
    12121 ccatcgctac cggctgggag cgcactatga agcgctaccc gtgaatgcgc cgaagtgccc
    12181 ggttcgccat taccacaagg atggtcccat gcgattcttc cagaacgata cgggcaaccc
    12241 ggatgcctac tatgagccga acagcttcca ggggccggcg caggatccgt cggtcgctga
    12301 gccgccactt cggatttccg gtgacgcttc ccgctacgac catcgcgagg gcaatgatga
    12361 tttcagccag ccccgggcgc tcttccgctt attcgacgag ggtcaaaggg aaagactgtt
    12421 cagcaatatc gccgacgcga tggacggggt accggaagcg attgtggaac ggcagtgcgc
    12481 actcttcgat caggtggatg gtgactatgg cgctggtgta cgcgcggcgc tcgcacggaa
    12541 aaaggcgaag agttcttccg ccaaggcagg atagacgggt gcgtttgctg ttcgcctgag
    12601 atcctttccg cctgggtgca cctactactt agctgcccag gcggagaact atgaataacc
    12661 caacggcaat tcaggcttca gcggaacacg gtgccgcgat tgttgcactc gaagcggaac
    12721 acgacgtaat tgacctggag cttggaaccc tgaagagcgc cattctgtcc ggagcggaag
    12781 gcgccctgat catttatgtt ctggaccgct cgatcgcttt ttgcgaagaa cacttcacgc
    12841 atgaggaaga cttcctgcgc gaccacggtt acgggcgggt ggacgatcac gccgctttgc
    12901 atcagcggct tctcaagagc tttcggtgcg cccgcgacct ggcgaccggt gacagcctgc
    12961 ctttggccgc gttggatgcc ttcgagttgt tgcaggagtt caaagagcac gtggaacgct
    13021 acgaccggcc tgcctacatg gaggtggaga agcggctgcc gcaaaaattc aaaaccaatg
    13081 gagctttcgc agcgcataca gcaatccgct agtggtgagg accatgagcg cggccaccac
    13141 agccagcgcg tagggtgaat cagcccaggg cagatggtgc agattcattc cgtagaaccc
    13201 ggatatcacc agggcgggca gcgcggcggt gccaagaacg gtcagcactt tcatcacctg
    13261 gttagtgcgg ttagccacgc ttgaaagata aatatccatt gagccggtga gcaggtcgcg
    13321 ctgcatatcg atggtgtcca gattgcgggc gatgtgatcg tagacgtcac ggaaaaacgg
    13381 ccaaagctgg gggtccacca gttccgtgcc gctgcgttgc aggtgagccg ttacatcacg
    13441 ggtatttgcc atcacgcggc gtatcgtgat gaggcagcgt ttcgtagaga gaactttctg
    13501 gagggtgcga ggttggggag actccagcac ctcgtcctcc agacgatcga tctcgtcgtt
    13561 caacccgtcc aggacaggag cgtaggtgtc cacgattccg tccatcacgc ggtagaatgc
    13621 ctggtccgga cggagccgtg cctcctgccc gcgcacctgg tttaaaatct ccctggcgct
    13681 cggacaatct ccttcctcca ccgtaatcac aaaatctttc ccgatgaaga gatccagatc
    13741 gaatacgtcg agcgattggt cctggttgag cagaacaggc ttcagcacaa cgaagaggta
    13801 gccgggccct tcctcaattt tggcgttctg gttccggtgc cggcagtcct caatgtggag
    13861 cggatgaagc tggtattgtg cagcgaggcg gtcaagctcg gggtccccgg gatcgcggat
    13921 atcgtgccat gccacgatga ctacgatagc aataccgcca agcggtattt cgaaagcgtg
    13981 ccgatgccgg tacgcggttt cctctatgat ctcaatagga gatgatactc aagctcaagc
    14041 gcacgcccgg catctacctg gtgggcttca tgggatcggg aaagtccact gtgggacggc
    14101 ttctggcgga ccggcttggc tggaacttca tcgacgtgga cgagcagatc gaagcggagc
    14161 agggagtccc catttttgag atcttcgaga agtccggcga gcaggagttt cgcagaattg
    14221 agcgcgaggc gatagagcgg cgggtcggca tggtggaagc gggacatccc atggtgctgg
    14281 cgctgggggg cggcgcgttc gttgaacctc acaatcggga tctgctgaag gagaacggga
    14341 tcacgatctg gctcgattgt ccttttgcga cggtgaaggc gcgcgtcgca cgtgctgcgc
    14401 acagaccgct ggcgcgcgac ccgaaacggt ttgaggacct ctatcacaac cgcagggcag
    14461 cctatgaaca ggcggattac aggatcgaaa ttgacggtga tgatccggaa cagcctgtcc
    14521 gctcgattct tgccctgccg attttttagg agcttgttga aaaacgctga cccagaaacg
    14581 gagccgcgcg cgtaagaagc tgtgaagaaa ttgaatttag ccacggatga acacggatga
    14641 acacagataa gtttcctgtt ttcaatctgt gttcatctgt gtccatctgt ggctctgaat
    14701 ctttcacacc ttcgaggcaa gcggttttct agatttttcg ctggcggagg cttgcctcgg
    14761 gaatgaatac ctatgactga atccactctc agaaagcacg cgctacagat ctttcaagcg
    14821 gcgttgaagg cggctgaccc ggtggaagcc gttcgccggc acgttcgcgt ggaggacgat
    14881 gttctcctgg tggacgggag gaagtaccgc ctcaagaact tcgagaagat attcgtcgtt
    14941 ggcgccggca aggcgggcgc ggccatggca aaggctgtgg agacgctgct cggcaggcgg
    15001 atctcgggcg gattcatcaa cgtgaaagac gggcatacgc ttccgctccg gcgcattgaa
    15061 ctgaatgagg cgagccaccc ggtgcccgat gagcgcggtg tgcggggagc ggaacgaatc
    15121 gccgaaatcg cgtcgcgcgc gggggaaaaa gacctggtgc tctgcctgat ctcggggggc
    15181 gcttcggccc tgatgccggc ccccgccgcc gggttgacgc tcgaggaaaa gcggcagacc
    15241 acgaaacttc tgctgaattc aggcgcctct attcacgaga tcaattcggt gcgaaagcac
    15301 ctgtcgttgc tcaaaggagg gcaactggcg cgcctggctt cgcccgccac ggtattgagt
    15361 ctgcttttgt ccgacgtcat cggggacgat ctggacgtga tcggctcggg cccggcggcg
    15421 ccggactcct cgaccttcgc ggatgcgcga gacgtgctcg aaaaatacgg catctacagt
    15481 cacgttccgc cggcggtgaa gcagcgaatc gaagacggaa tctccggtgt tgtggaggat
    15541 acgcccaaat ccggcgatcc gatcttcgag cgtacgcgca acgtggtggt tggaagcaac
    15601 cggctggcag tggatgcggc ggtgaaacgc gcccgtgagt tgaacctgaa accgctggta
    15661 ttgtcgacct tcatcgaagg cgagacgcgg gagatcgcgc ggatgcatgc ggcggtagcg
    15721 aaagagatcc tcacttccgg aaggccgttg aagccttcct gctgcctgat ttccggcggg
    15781 gaaaccacgg tgacgattcg aggcgaagga ctcggcggac gcaatcagga attcgtactg
    15841 gccgccgcca tggatatcgc cggccttcga aacgttgtcg tgttgagcgg aggcaccgac
    15901 ggcacggacg gaccgacgga tgccgcgggc gcgattgcgg atggcaacac gatcgcacgc
    15961 ggggacaagg caggcatgag cgcaagcaac gcgctttcct ttaacgactc ctatcacttc
    16021 tttgaaccgc tcggcgatct tatcaaaacg gggccgacca acacgaacgt gatggacatc
    16081 cggctggttc tggtggcggg ctgacacatg tcgttctggc gcagtgagct gatcgatttt
    16141 gctttgaggc aggagacgga aaagcacatc tgcgaacaga tggagtggat cgcgcgcgac
    16201 cctgagaacg ccacacctta ctacaacctg gcacagctat accggatcga gggccgggcc
    16261 gaagaggccc tggggttgtt gctcgaagcc gtgcgcctcg acgcggagtt cgccggggcg
    16321 cacgttgctc ttgccgggat gtatgcggtt aagggggatt atccggcggc ctggaaacac
    16381 gcgcgtagcg cggagagctg cgggaacagc gcgggcatcg aactgctctc ccgctacggc
    16441 gtagctactg atcgatgaca gtgagcgagg tcgccctggg cggctttggt gcttttggcg
    16501 gcaaaggagg gttgctttgc tcgaatgcgg aactctttcc aacggacacc gtgccacggt
    16561 ccgatgccag gttgagtaag ggacctttgc ccaccgcgcc cgccagctca aagctgcggc
    16621 cgttttgagt tcgctcgagc ggtggaccga aatcgttttc gatatcgccg cgccgggaga
    16681 ccgccttgac cgtaaattcc gctttcggcg gcagcgctac ttcgatgttg ccggaatgcg
    16741 agcgtacatt cattggcgac agtggcagtt tcaccgggtt taagtggata tctccacgat
    16801 ctacggagat ctccagcgct cccgtaaaat tcgagaggtc gatgtcgcgc gaacgggcgg
    16861 agatcttcac cgggcccacg aggttcgatc cgctgacctc cctcaggtcc atccggagct
    16921 caccggaaag ctgttccagg ttgaactcgt tttggccggc actccaccgt agagactgct
    16981 tcagattacg gagttggacg tcgccataat agctggccgt tatggtgatc ggaccggcga
    17041 cggtgtccag ttccaggtcg tgtcctctgc ctttcagttc cacgctcgca cccgcaccgc
    17101 tcgcgcgaat aatgtcgctt gcacgagtct cgatgcggat ggccccgccg atattggcga
    17161 gccgcactcc cgcgttatcg ctcttgattt ccacgccgcc gtcgagatcg gtgatgtcaa
    17221 aatcgccgcg ccggcctttc gcttcgatcg atgccccgcg cgggaccgtg atctccaaat
    17281 ccgtcgaaac gcggttttct ccggaggccc tctcctgcat ggtgcgaatg aggacctgct
    17341 ctccctgggc cgtcgcctca agcggggttc tcttgtcatc cgcgtcggct tcgctgcgtt
    17401 gaagcgcacg aacggtcttg cggccggcaa ttttcacttc ggcgatgtcg gcgccgatga
    17461 tacgtgtgtt gccccgcaga ttttcaataa tgaccctggc attcttgggc gccggtacgc
    17521 tgcgctcggc caaagggtag tcgtagggtt cgccgaagac ttcaatgccc cgcgccataa
    17581 tccgtgcgcg cggccaccac cagtcgccgc gctcgtggac gaaacgcgcg ccggagccga
    17641 tcagcaccag aaagaccacc agggtccact cgcctccaga aacaccggag gttggcagcg
    17701 ggcgcgagcg ggcagcccag atgacgattt caaacagtcg caggacgccc catccgacga
    17761 gcaggtaagg ccagaaacgc gacaggagat cgatgatacg caaatcgggc cgcaagttgt
    17821 atagcaggaa tgccacgccg atgaggatca gcaccatcgg cccggcgatg gagcggggtc
    17881 tcatgacaac ctctctggcg gagaactggg gacgggagcg ggctgccgga tgacggcccg
    17941 ctccagcagt ttcaagagcc caaacagaat gatgagcacc ggccacgtct ggcggaacgg
    18001 tggtccgccc gcgtggtcga cgatgaacag gacgccgaga gcgatgagca ggatgggccc
    18061 gcggatcgcc tgaacgaacg ccagtctact gttgttcatt gctcgcctcc cgccgcaccg
    18121 cctcgccttc ggagtcctta aacctcacat agagcatgta gccgccgagc gcgatcagaa
    18181 acaccggcca atagcgcaat acgtaataga agttgagcag atcgagatta ttcagcaaaa
    18241 acagcacgcc gagcacgatc agtacaaccg gggcaacggg tgaattggta tgtgtgccgc
    18301 gcatcggaac caggctggag aactcgtcta ccggctggcc caattgccgt ttcctggctg
    18361 tgtggtaggc ctcgaacggc atgtaaaagt accagaccgc cgtgacaaga ccaaataccg
    18421 gctccaggcc gcccgcggcg ccgctgctga ggatgctgat caggaacccg agaatgatca
    18481 cgtggatcag ccccttggca tactggccgt tgtaaatagc tcccacgcct ggtatcagac
    18541 ccagcagaaa ggcgagtccc ggggatgcgc cggggtcggg cgatcccggt gcgggcgccg
    18601 gggccgtcca gggagaatcg gaagggggcg ccaatgccgg ctgcgccggt acgtgttcct
    18661 cacagtagat tgttccctgg gccgtacgag cgcagttctg gcatagggcc ttgccgcagc
    18721 tccggcagta cgctgccgcc gctaattcag gatgattgac acagttcatt ggtttgacct
    18781 tcctttgggc gccggctccg aagaaccgga cgcgcctgac gtatcaccgg cgccagtagc
    18841 gccggccggg ctgttggagc ccggagtagc cgaaggagcc tgtacggcgg ggtccgtcgc
    18901 cgtcagatct ttcagccggg attgaatctc gacaaagagc cgcatgctct cgtaatactt
    18961 cacaccgcgg tcccaaacgc gatgaaccct gtcttccacc accacccaca ctttttccgg
    19021 ccggaggtcg tctaccgtca actgccggat gggaatattc aggaactggc tgagcatgga
    19081 aaaggacaga atggtcatcg ccatccccat ggcaaagcgc ggctggagga tggggccgaa
    19141 aatccggtgg aagcgtgcca tagctccccc gcggcggcgc aagccggcgg ggatgctgaa
    19201 cgcgatgcgg gtcatcagtt cgtgcggcgg ctccacggcg ctggcgcggc caataaaggt
    19261 gaccgccagt gaacagtccc gtgccagccc ggcgcaagcc tcacattcgc cgagatgctg
    19321 ttcggcgccg gacttgcggt ccggtgcgag ggtgccgtcg acgtagtcgc agagcagggt
    19381 ctcgaattcc gcgcacttca tacaactacc ctctgccttc tcaacacacg agccaattcc
    19441 gcccgtcccc ggttcaaacg cgatttaacc gttccttccg ggactcgcag gatcccggca
    19501 atctccttgt actctagctc ttctatatcc ctgaggatta ccgtctcgcg caactcagga
    19561 gaaagtttct ggagcgcctg ctgaagtagc tcgctcgcct cccgccccgc cagggcgcca
    19621 tcgggcctcg ttgacggtgt ggaactcacc tctaacaccg ccagttggtc ctcgatggaa
    19681 tcactggccc ggtccatgcg gctgcgccgg tagtggtcga tcagcaggtt gcgcgtcagc
    19741 cgcgtcagcc atacgacgaa agaaccttca ccggcgcgaa aacttccgat ggaccggaac
    19801 acgcgcagga acacctcctg cgtaaggtcc tgggcttcac catcgctgcc ggtaaaacgg
    19861 tagcagatgc cgtagatgcg gcgggtatga atcttcacca ggtcttccca ggcggattcg
    19921 tcgccggcca ggcatcgctc gaccaggttt gaatcaagat ccaacggttt ccccggcaat
    19981 tggaggcagc cccgccccgc cgtctcttcc gtccaccgga ctgaggaaga gtccgcggtg
    20041 gacatttgcg caaatgcggt aaaggctgcc ataaagatct tcgttccgga agcccgaccg
    20101 aaggtccagg actgcgaatg ctttcttcgg cttccagtaa gttaaacgag gatctatcat
    20161 tagaagttcg ctcgaatgta cgtcccggaa aatgggacgg caccggggcg ccgtaattct
    20221 gtttccgtgc agattttatt ctattgcagc agcggccaac ccgatgaggg gaagatgggc
    20281 ttacgccgtc caccggttcg cggggagggc gtatccggat aaacaagaag cgtaccgcca
    20341 tgatcgccat cggagtggtt tgcgtggcgg cggtcgtcct tgcgctggtc aggctgcgag
    20401 gtttcgattg ggacaagttc tttgccgctt ttcggaatct caactggggg tggcttaccg
    20461 cgtcggcgtt gatggcgctc gggacgtacg tggtgcgcgc ggagcgatgg cgggttatga
    20521 tccggcccat ttgcccccgg gcgagtttct ggggtctgtt ggtgtccact gccattggat
    20581 tcacggcgct ggtgctcttc ggccggccgg gggagtttgt gcgtccatac ctgatcgcaa
    20641 ccaagaacaa gctcacgata acctcgcaag tcgccgtgtg gttcctggaa cgaatctacg
    20701 acctgctgat tgttctggta ttgttcggat ttgcgttgac gcgcgtgcag gcctccggcg
    20761 ccagtcttgg tcccgatatg cggtggctgc tcgaaatcgg cggacacatc gtgggcatag
    20821 cgggcactat ctgcctggtg gtcctgatct ttctgcgtta tttctccgag accttccggc
    20881 ggcggattac cgatgcgctc ggggtcctgc cggctcgcct cgaggaacga gccgtgaaaa
    20941 ccgtcgatgc gctatttgaa ggcgtgcagt cggcccgaag cggaacgttc gttctacagt
    21001 tggtttttta cagcattgcc gagtggattg ttattaccgc ttgttttctg tttcttttct
    21061 ggagcttccc cgatactgcg gcatttacgc tgaccgatgt tcttatattc gtgggattcg
    21121 tcgcgatcgg aagcgtcgtg cagatacccg gaatcggggg cggtatgcaa gttgcatccg
    21181 taattgtgtt gagcgaggtt tttggcattg ggttcgagtc tgccagcgca cttgccatac
    21241 ttctgtgggc tattacattt ttagtaattg tgccgattgg gcttttgctg gccttccacg
    21301 aggggttgaa tctgcgtaag ctgcgcgaca ttgagcaaaa gagcgagata tgatctgtcc
    21361 gttttgcggt tttcgccagg accgggtgat cgactcccgc gagagcaagg agggcgacgt
    21421 catccggcga cggcggcagt gcctggaatg tgagcgtcgc tttacgactt acgaaagaat
    21481 tgatgaaatt ccctacatgg tcgtcaaaaa agatggccga agagagaagt tcgaccgtca
    21541 aaaagtattg ggaggattgc tgagagcctg cgaaaagcga cccgtgccca tgggcaaact
    21601 ggcggaagta gtagacgagg tggaaggcag gctggcggag acccaggatc gcgagctacc
    21661 tactacaata ataggagaaa ttttgatgga gcgtttgcgg acgttggaca aaattgccta
    21721 tgttcgcttc gcatctgtct accgggattt tcaggatgtg gaggcctttt tgaatgagct
    21781 gaaaaacctg attgtgcaca agcgggagaa gcgcccgttg tgatgggcaa tgacggtgtc
    21841 aacgttgcaa aaaggcgatc attaggtgta accaaaacgt tccggaaaca tctttcaaat
    21901 agagacttaa cttcgctgcc gatcggtgct ataatcgaac ccgaaacaaa gctattgaaa
    21961 gcaagggcct tttgtgtctt cgattctgac ctgctcggcg ggagtcagtt tgttgttgtg
    22021 ccctaggaat ctttcacggt gggggaagtg agtcggaagt ggatcatttc gaagatcaat
    22081 atataggcga tagccatgag ccgttagggc ttacctttga cgaggaaccc aatttttttc
    22141 acccggagga agcgcatgac gaggagttcc cggctgcgca ccaggtggaa gaatccatct
    22201 acacggacga ccctgtccgt gtataccttc gggagatggg tgctgtaccc ctgctgacca
    22261 gggaaggcga ggtcgacctc gctcacaaga tggagcgtgg aaagctccgc atgcaaaagg
    22321 ctattagccg gtcggccctg gtacagcaga tggtcgtcga gtacgccgaa caactcaaac
    22381 gaggcgatat cgacctggag gatctggttg acctcggcga tgtcgaagag ggtagcgccg
    22441 cggaggcgaa gcgccggagc gatgtgcgcc agcagttcgc cgatgtcgtc ctgctgtgca
    22501 ggcggaaaca gcagctcacg gaaaagctga acgcagccaa cggaaacaac aagaaaatcc
    22561 ggaagcgggc ttccggcaag ctgaaccgtt gccgcgtcga gacgtcgcgc acgatccggg
    22621 cgattccgtt caacgcacat cgctggcggg agtttgccaa agagattgag cgcacggtgg
    22681 atgacgtcgt gcaactggaa gcggagcagc ggaagctgga ggagaaaggc aatcccgctc
    22741 tgcaggccag aattcgagaa atccggcggg aaatccgcaa gcgggaagcc gtcgcgggcg
    22801 cctccgttcc ggatctgaag catagcctga gcgtgatccg gcacggcgag caggaagccg
    22861 aacgggcgaa ga
//
LOCUS       DASTMK010000066         7655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_67630, whole genome shotgun sequence.
ACCESSION   DASTMK010000066 DASTMK010000000
VERSION     DASTMK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67630"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFQ79_02715"
     CDS             complement(<1..355)
                     /locus_tag="VFQ79_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU0138589.1"
                     /translation="MVAAGRKKSIAFFIALGAGLVTVILVLYIGWILLNWRTGILLFL
                     GILLVAMIIGGVVVNTLFLVREILRNEQHNAFINAMTHELKTPVASIRLYLQTLQTHP
                     VDEEKRKDFYRIMLED"
     gene            complement(406..1263)
                     /locus_tag="VFQ79_02720"
     CDS             complement(406..1263)
                     /locus_tag="VFQ79_02720"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEU0138590.1"
                     /translation="MVNKDIVNIYDRQKLNWVTTAVLILLHIGAVAALFMFNWASFAM
                     AVFLIWMATGLGISMGYHRLHTHRSYKVPLPLEYFFAVCGALTLEGGPIFWVATHRIH
                     HQKSDQPGDPHSPRDGAWWSHVGWILFGEAKHNNTRLMSKYAPDLAKHRFYVWLNDYH
                     WLPLVCLGVILFAIGGLPMLLWGGCLRVVVGLHATWLVNSATHMWGSRRFNTRDDSRN
                     NWWVALITFGEGWHNNHHAHPTSARHGLTWFEFDPSWLQIKLLKYLGVAKAVHVARPD
                     SRVAEREAA"
     gene            complement(1336..1833)
                     /gene="tadA"
                     /locus_tag="VFQ79_02725"
     CDS             complement(1336..1833)
                     /gene="tadA"
                     /locus_tag="VFQ79_02725"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEU0138591.1"
                     /translation="MTSFDRPLPEDTRFMAAALDLARQAQQTGEVPVGAVVVRNGEII
                     GQGFNSVIARNDPTAHAEIVALRLAAARLENYRLDGADLYVTLEPCVMCAGALVHARI
                     RRLIFGCRDLRYGGVRSKFRLADSEILNHRVPVVEGILAVECVEILKDFFDARRVARP
                     DDSVR"
     gene            1922..5383
                     /locus_tag="VFQ79_02730"
     CDS             1922..5383
                     /locus_tag="VFQ79_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0138592.1"
                     /translation="MSTRSLLLTGVILLWPGWMSGQDPRGALRGRVTDQSGALMANVD
                     VRATNVASGVAVASRTNAAGVYNIPFLLPAIYNVTAELQGFKKFTQTGVQIRVAEVTE
                     LNIAMEVGGITETIEVKDETPLLDTASSSLGQVVDQRRILELPIAAGNPLELVLLTPG
                     MVEPSKFLWKPAWNFRNVTSDGNPAYTTEYTIDGVSNTFAEGNAGRSRYAFAPPATAI
                     REFKMQTAAYDASVGHTLSSVVNVATSSGGNEIHGQAQWAVRNKAFDAPNFFNNRNNT
                     KMPVYQDNRYGASAGGPVYLPRLYNGKNRTFWHYTWEANKWDVPQSYTGTVPTQAQRQ
                     GDFSGLLAINNSYQIYDPLSIAVAPNGRFSRQPVPNNIIPTNRLDPVGLNLAKLYPLP
                     NASGTIDGRNNYFNGSVNALEDYYVHLARVDHTFSESHRVFVRFHYDWWEEDKDRRFG
                     PESPANGIVLERINRGLALDDVLLLNPNLVLNIRYGLTHQEFLERRISRGFDLTSLGF
                     SPSLANLIDGSLATIPNVNAGAYAPISRWESGDGATTSMTHSLAGHFNMMRGPHNVRF
                     GADYRVYRGNNNRFPQAVSPVLSYSNYWTRGPLDNSPAAPIGQELASMLFGIPGGGME
                     RTASFAMQDQYLGLYIHDDWKLSRNVTLNLGLRYEIETGVTERYNRFVAGYDFVTPNP
                     VEAQARENYARAPIPELAPDQFHAVGGLTWVNEGGIGRSPFRTEKNNFMPRIGLAWQL
                     HPNTTFRAGYGIYYDTIGVNATRPIQTGFTQGTPIQASLDEGLSYIATNANPFPSGLL
                     EPRGSAGGLRTNLGQSLEFYDPNRTHPYSQRWSGGFQQLLPAQFLADVSYVASRALRL
                     SIPRSLNDTPARYLSNLPFRDQQTINYLSQSFPNPFSGTDPIYGTTTSRAALLRPFPQ
                     FGSMSRMEPIGYSWYHSLQIRGEKRFSQGYTFQLGYTWSKLMEAVSFLNAVDAMPYEV
                     IGSFDRTHRLTMSGIWEVPFGRGRRHGAQTPAVVNFIAGGWQINGVVVRQSGAPLGFG
                     NVIFNGDLKDLVLPKSERTVDRWFNTDAGFNRNSAQQLASNLRGFPARLSGLRGDGRA
                     TWDFSAIKNFNIRENVVMQFRAEVYNAWNHANFNDPNTDPVSTAFGRITSAAEGRNWQ
                     FGLRLKF"
     gene            5601..6422
                     /locus_tag="VFQ79_02735"
     CDS             5601..6422
                     /locus_tag="VFQ79_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138593.1"
                     /translation="MRLRWSQNVTLAFVFVVAPVHAEDWGALFEALTSIDTQTSDAAR
                     KKVFGETLPFLLRADARAVSPEMQGIARQLAHHPRIRLQAIATLASVAYGRRDSAEVL
                     AGVLPELIAHLNDETARIRFDAAFALSHLKPSIPAEAIPHLINARRSRDASLAQGGVF
                     GAARLAEGSTDASEAVAAALRANHNAAAKQFILRAIAANRVVSVLVQDSISDLLETAT
                     DVNVLIEALNSATALGSAATERLVRNVDLLVTTTPAVPVAEAAAAARLKLQESRE"
     gene            6832..7029
                     /locus_tag="VFQ79_02740"
     CDS             6832..7029
                     /locus_tag="VFQ79_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138594.1"
                     /translation="MKVKRPPVEVLTGTGASSATQRAAAPEPDIVFDQNTVVNHGLYF
                     LLQVEDGSPSRIELDRTPADM"
BASE COUNT         1610 a         2295 c         2203 g         1547 t
ORIGIN      
        1 tgtcctccag catgatgcgg taaaagtctt tgcgtttctc ctcgtctacg ggatgggttt
       61 gaagggtctg aaggtaaagg cgtatggacg cgacgggcgt tttcaactcg tgcgtcatcg
      121 cattgatgaa ggcgttgtgc tgctcattcc tcaggatttc ccgcaccaga aataatgtgt
      181 tcaccaccac gccaccgata atcatcgcca ccagcagaat gccgagaaac agcagaatgc
      241 cggttcgcca gttgagcaga atccagccga tatagaggac cagaatgacc gtgacaaggc
      301 cggcgcccaa ggcgatgaaa aaggcgatcg attttttccg gcctgcagcg accatgatta
      361 caggttatgc cgccaggcat ctccctgcat cgggccgcca gcggcttatg ccgcttcgcg
      421 ttctgctacg cggctgtcgg gtctggcaac gtggacggct ttcgcgacgc cgaggtactt
      481 cagaagcttg atctggagcc aggatgggtc gaactcaaac cacgtgagac cgtggcgagc
      541 tgaggtggga tgcgcatggt ggttgttgtg ccagccttcg ccgaatgtaa tgagagctac
      601 ccaccagttg ttgcgggaat cgtcgcgggt gttgaaccgc cgcgagcccc acatgtgagt
      661 ggccgagttg accagccagg tggcgtgcag ccctaccacc acgcgaagac agccgcccca
      721 caacaacatc ggaagccccc ctatcgcgaa gaggattaca ccgaggcaga ccaacgggag
      781 ccaatgatag tcgttcaacc acacatagaa gcggtgcttc gccaggtcgg gcgcatactt
      841 cgacatcagc cgcgtgttgt tatgttttgc ctccccgaaa aggatccacc ccacgtggga
      901 ccaccatgcg ccgtcgcgcg gggaatgcgg gtcgccaggc tgatcggatt tctggtgatg
      961 aatccgatgc gtcgccaccc agaagatcgg tccgccctcc aacgtcagag caccgcagac
     1021 ggcaaagaag tattccaaag gcaatggcac tttatacgaa cggtgtgtgt gcaggcgatg
     1081 gtaccccatg ctgatgccca gccctgtagc catccagatc aggaagacgg ccatcgcgaa
     1141 cgacgcccaa ttgaacataa acagagcggc cacggctcca atatgaagca gtatgagaac
     1201 ggcggtagtt acccagttca gcttttgccg gtcgtatata ttgacaatgt ctttgttcac
     1261 catatcccca cgtgagtagc ccacgttctc aacctaacag ggatcgggcc ggtgttttgt
     1321 aagagttatg taatctcagc ggacactgtc gtcaggccgg gccacacgtc tcgcatcaaa
     1381 gaaatctttg aggatttcga cgcattccac ggccaatatt ccttccacga ccggcacgcg
     1441 atgattgagg atttccgaat cggcgaggcg gaatttgctg cgcacgccgc cgtaccggag
     1501 atcgcggcag ccaaatatca gccgccggat tcgcgcgtgg accagggcgc ccgcgcacat
     1561 gacgcagggc tccagcgtga cgtacaggtc cgcaccgtca agacggtaat tctccagtct
     1621 ggcggcggcc aggcgcagcg cgacgatctc cgcgtgggcc gtcggatcgt tcctcgcaat
     1681 gaccgaattg aatccttgcc cgataatttc gccgttccgg accactaccg cgcccaccgg
     1741 aacctcgccc gtctgctgag cctggcgcgc cagatccagt gcggcggcca tgaaacgcgt
     1801 gtcctccgga agaggacgat cgaaggatgt cattgttctc cattatcccg gctaaaggca
     1861 gccattgacg gaaaatcgcc tctgatatac tgaacgtgct tctcacgaaa aggacggtgt
     1921 tatgagcacg agatctctgc tgcttaccgg cgtaattctg ctgtggcccg ggtggatgtc
     1981 cggccaggat cctcgcggcg ctctccgggg ccgtgtcacg gatcaatccg gcgcgttgat
     2041 ggccaatgtc gatgttcgcg cgacaaacgt cgccagcggc gtcgccgttg cctcgcggac
     2101 caatgcggct ggggtctaca acattccttt cctgttaccg gcgatttata acgttaccgc
     2161 ggaactgcag ggcttcaaaa agttcacaca aaccggcgtg cagattcgag tggccgaggt
     2221 caccgagctg aatatcgcca tggaggtggg tggaatcacc gaaactatcg aagtgaagga
     2281 cgagactccg ctgctcgaca cagccagttc ttcgctcggc caggtggttg accagcgccg
     2341 gattcttgag cttccgatcg ccgccggcaa tccgcttgag ttggtgctgc tcactcccgg
     2401 tatggttgag cccagcaagt tcctgtggaa acccgcctgg aacttccgca acgtcacaag
     2461 cgacggcaac cccgcctaca ccaccgaata cacgatcgat ggcgtttcga acacattcgc
     2521 ggaaggcaac gccggacgca gccgttatgc gtttgctcca cctgcgacgg cgatccgcga
     2581 gttcaagatg cagacggccg cttacgacgc ctccgtcggc cacacgctga gctcggtggt
     2641 gaatgtagcc accagcagcg gcggtaacga gatacatggt caagcgcagt gggccgtgcg
     2701 gaacaaggct tttgatgccc cgaacttctt caataaccgg aacaacacga agatgcccgt
     2761 gtatcaggac aaccgttacg gtgcgtctgc cggagggccc gtctacctgc ctcgccttta
     2821 taacggcaag aaccgcacgt tctggcacta tacctgggaa gcgaataaat gggacgtgcc
     2881 ccagtcctac acaggcaccg tccctaccca agcgcagcgg caaggcgatt tttccgggct
     2941 gctcgctatc aataactcgt accagatcta cgatccgctc agcatcgccg tcgctccgaa
     3001 cgggcggttc agccgccagc ccgtaccgaa caatatcatc cccaccaatc gtctggaccc
     3061 ggtcgggttg aatctggcaa aactgtatcc gctgccgaac gcctccggca ctattgacgg
     3121 ccgcaacaac tacttcaacg gctccgtcaa cgccttggag gattattacg tccacctcgc
     3181 ccgtgtggat cacaccttca gcgaatctca ccgcgtgttc gtgcgtttcc attacgactg
     3241 gtgggaagag gacaaggacc gccgcttcgg gcccgaaagt cccgctaacg gcattgtcct
     3301 ggagcgcatc aatcgcggtc tggcactgga tgacgtgctt cttctaaacc ccaacctcgt
     3361 gctgaacatc cgctatggcc tgacgcacca ggaatttctg gagagacgca tcagccgcgg
     3421 atttgatctc acctcgctcg gcttctcccc ctcgctggcc aacctgattg acggcagcct
     3481 ggcgaccatt cccaacgtga atgcgggtgc ctatgctccc atttcgcgct gggagagcgg
     3541 ggatggcgcc acgaccagca tgacgcacag cctggccgga cacttcaaca tgatgcgcgg
     3601 cccgcacaac gttcgcttcg gcgccgacta ccgcgtctac cgcggaaaca acaaccggtt
     3661 cccgcaggcg gtgtctccgg ttctctctta ctccaactac tggactcgtg gtcccctcga
     3721 caattcgccg gcggcgccca tcggccagga actggcgtcg atgctgttcg gtattcccgg
     3781 cggtggaatg gagcggacgg ccagtttcgc catgcaggac caatatctgg gattgtatat
     3841 acacgacgat tggaagctgt cgcgcaacgt gaccttgaac ctggggttgc gctacgaaat
     3901 cgagacgggc gtcaccgaac gctacaaccg cttcgtcgcg ggctacgatt tcgttacgcc
     3961 gaatccggtg gaggcacagg ctcgcgagaa ttatgcccgg gcgccgattc cggagctcgc
     4021 gcccgatcag ttccatgccg tcggtggtct tacgtgggtc aatgaaggcg gtatcggacg
     4081 cagcccgttc cggacggaga agaacaactt catgccgcgc atcggtctgg cgtggcaact
     4141 gcatccgaac acgaccttcc gtgcgggcta tgggatctac tacgacacca tcggtgtgaa
     4201 cgctacgcgc cccatccaga cgggcttcac tcaaggcacg ccgatacagg cctcgctcga
     4261 cgaaggcttg tcgtatattg cgacgaatgc gaacccgttt ccttcgggat tgctggagcc
     4321 tcgtggatcc gcgggcggac tgcgcaccaa cctcggtcaa agcctggagt tttacgatcc
     4381 caaccggacg catccttact cgcagcgctg gtcggggggc tttcaacagt tgctgccggc
     4441 gcaattcctg gccgatgtct catacgtcgc cagccgggct ctgcggctga gtatcccaag
     4501 gtcgttgaac gatacgcccg cccggtattt gagcaacctt cctttccgcg accaacagac
     4561 catcaactac ctaagccagt cgttcccgaa cccgttctct ggaactgatc ccatctacgg
     4621 aaccaccacg tcgcgagccg ctctgctgcg gcccttcccg caattcggca gcatgtcgcg
     4681 tatggagccg ataggatact cctggtatca ctccctgcaa atccgcggtg aaaagcggtt
     4741 ctcgcaaggc tacacgttcc aactcggcta cacctggtcg aaactgatgg aagccgtctc
     4801 tttcctgaat gcggttgatg cgatgccgta tgaagtgatt ggctcgttcg accgaacgca
     4861 ccggctcacc atgagcggta tttgggaggt gccgttcggg cgcgggcggc gccatggcgc
     4921 gcagacgccc gcggtggtga atttcatcgc cggcgggtgg cagatcaacg gtgtggtggt
     4981 tcgccagtcg ggcgctccgc tcggtttcgg aaacgtcatt ttcaatggcg acctgaagga
     5041 tctcgtactg ccgaagagcg agcgcacggt ggaccggtgg ttcaataccg atgccggctt
     5101 taaccgcaac tcggcgcagc aactggcgtc caatctccgc ggttttccgg cgcgcctgag
     5161 tggcctgcgc ggcgacggcc gtgccacgtg ggacttttcc gccatcaaga atttcaacat
     5221 ccgcgagaac gtcgtaatgc agttccgtgc cgaggtctac aacgcctgga accacgcgaa
     5281 cttcaacgac ccgaacaccg atccggtcag cacggcattc ggccgtatca cctccgccgc
     5341 cgaaggccgc aactggcaat tcggcctccg gttgaagttt taaccaggac cggcaacatg
     5401 ccaagccatg aacgcccatc gaatttatga gattcgatgc ccggcgcatg ggtttgtcgc
     5461 gctgaatgac tgaagagtcg gccactttat caatttgctg tggccgggaa tgatccgtat
     5521 atccacattg ggccctgtct tctgggttta gggcaagtga cggatatccg ttcggggacg
     5581 ctccagtgag gagaacttca atgagattac gatggagcca gaacgtaacg ctcgcctttg
     5641 ttttcgtggt tgcgcccgtt cacgcggaag actggggcgc tctctttgag gcactaacca
     5701 gcatcgatac ccaaacgagc gacgcggcaa ggaagaaagt tttcggtgag acgctgcctt
     5761 tcttgttgag agccgatgca cgggcggtct ccccggagat gcaaggcatc gcccggcaat
     5821 tggcacacca tccccgaatc cggctgcagg cgatagccac gctggcttcg gtcgcttacg
     5881 gaagaaggga tagtgcggaa gtcttggccg gtgtgctgcc agagttaata gcacatctca
     5941 acgacgagac tgcgcgaatc cggttcgatg cggcgtttgc gctttcgcac ctgaagccgt
     6001 cgattccggc ggaggcgatt cctcacctca tcaacgctag aagaagccgc gatgccagct
     6061 tggctcaagg cggcgtattc ggcgctgcgc ggctcgcgga aggatcaacc gacgcttcag
     6121 aagctgttgc tgcggcactt cgagcgaacc acaacgctgc ggccaagcag tttatccttc
     6181 gagcaattgc tgccaaccgg gtggtatcgg tactagttca ggacagcatc tctgacctgc
     6241 tggagacagc gactgatgtc aacgtgctta tcgaggcact caattccgcc acggcactag
     6301 gttctgcggc gacggagcgc ctggtgcgga acgttgattt actcgtgacg acaacacccg
     6361 ccgtgcctgt tgcagaggcc gcagcggcag cccggttgaa actacaggaa tcccgtgaat
     6421 gatggcgagc cgcaaacagc gctcacggct tgccagcgga gtcctcgcgg tagtcctgtt
     6481 agggtgtact ctgacagcgt gttaccgaac gattcgtgtt accgaggaga tgcgatggga
     6541 ttgtggtccc ggtttgccga tggaaccgcc gtgtgatttg cggttgcgct atgtcgaatc
     6601 gccatcgtac gtagagtatg tcgcggacaa ttcgatttgt agcacgctgt cgaaatccaa
     6661 tcgggaaacg gtgcaggtgc aattcgaagt gcggggaaat cgatgggccg ggctgaaggg
     6721 ataccacatt gttgcgatag gccacgtcgt gtctttgtcg cccgggcata tagggagcgg
     6781 acgtgagggc ccgcgcacca gcccgaaacc attgagcaaa tattttcaga ggtgaaggtc
     6841 aagcggcctc cggttgaagt tttaaccggg accggcgcgt caagcgccac ccagcgtgcc
     6901 gcggcaccgg aacccgatat cgttttcgac cagaacacag ttgtcaacca cggcctgtac
     6961 tttctccttc aggttgagga cggctcgccg tcccggatcg aactggatcg tacccccgct
     7021 gacatgtgaa atttcgctat tccgtatggt gagattcagc ggggggccgg tgattgcggc
     7081 tgctgcatct tcttgatctc aacgttgagg agacggggcg tcacaaaaaa acccgtaata
     7141 caaataaagc gctcttgccg caggccgtgc tcgaactcac tgatcaacca cagatccact
     7201 tgcaagcgcg tcaaggatgc cggcgacagc gcgtggccta tggaaagtgt gcggaggaat
     7261 gtgccatgct cgcggcgtag attgctaagc caagcctcag agttccccac acgcgcacac
     7321 agggcgccaa ccccggcggc gtcctggttg tataaccggt ggaagttctc aacaagcgct
     7381 agacaagaac gtggcgccgt aaaaaccata ctcattattg ctggtcacaa cttctcttaa
     7441 tcccttccag tcttttcctt tcatacacct ctgcgcaaca tcctaaatct cttttccggc
     7501 gccgatgtat gttaagagcg aggagttgct cgttggccga gaggcaaccc accgagaccc
     7561 gcgcaggact accccgggga gtgtaaaacc tgagaccgcg gcgcgtcgac gtgacaggcc
     7621 tgcctaagtc ctcccataca ccctttgcgc ggggg
//
LOCUS       DASTMK010000067         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_259478, whole genome shotgun sequence.
ACCESSION   DASTMK010000067 DASTMK010000000
VERSION     DASTMK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259478"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..389)
                     /locus_tag="VFQ79_02745"
     CDS             complement(129..389)
                     /locus_tag="VFQ79_02745"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HEU0138595.1"
                     /translation="MGQECRELLSIQRHPGEACAQVRDLIAAQLAVVRARISDLQKLE
                     TDLSEALRQCGAALRKPPMQRDCCPVLQEISASRPHRKAPCG"
     gene            complement(631..1836)
                     /locus_tag="VFQ79_02750"
     CDS             complement(631..1836)
                     /locus_tag="VFQ79_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEU0138596.1"
                     /translation="MTDLAGIKKRFSVLSRLLDERSRRLVAGAESMALPRGGISAVSR
                     ATGISRPVIRQGIAELKNPKTALVGRVRRPGGGRKRIAEQDAAVLVDLEKLVEPVTRG
                     DPESPLRWTCKSVRRLAEELGRQGHVISYPVVAELLRELGYSLQANRKTTEGDGHPDR
                     NAQFEYINRKVQRFFASKQPVISVDTKKKELVGDFKNSGRELRPKGKPEDVRVHDFLI
                     PELGRATPYGIYDLAQNTGWVSVGVDHDTAAFAVESIRRWWQSMGQPVYPKAHRLLIT
                     ADAGGSNGPRLRLWKVELQKLADETGLRIAVSHFPPGTSKWNKIEHRLFSFISQNWRG
                     KPLLSYEVIVNLIAATTTAKGLKVYAELDSGSYPAGTKIEDHELAGVRLRRDKFHGDW
                     NYEIHPHGV"
     gene            1927..>2921
                     /locus_tag="VFQ79_02755"
     CDS             1927..>2921
                     /locus_tag="VFQ79_02755"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEU0138597.1"
                     /translation="MLFCQLGRADSLREICNGLGCCLGRLVHVGITHAPRRSTLSYAN
                     EHRPAALFEELFWTAAARFRNQQALGSRKHKFRFKNKLLSLDSTTISLCLTMFPWADF
                     RRAKGGVKAHVLLDHDDLPAYVLITEARRSDVKLADSFRLNAGSIVAIDRAYIDYALF
                     ARWSMAGVFFVTRMKDNAAFEVVEECQLPANRNIRVDQLIRLTAAQARADYPELLRRI
                     VAWDAENECEIVLLTNLLEFGSTTIAAIYKERWKIELFFKALKQNLTVKTFVGTSENA
                     LRIQIWTALIALLLLKWLHHLSKAGWSLSNLASMLRLNLFTYRDLTKWLADPMETP"
BASE COUNT          550 a          978 c          773 g          620 t
ORIGIN      
        1 ttccgccatg gcgacgtcct tcacctcgat ttcaacgatt tccgccggca cggcctcgag
       61 cgcgagcgct tcccgcatac gatcgcgggc cgggacgtga ttcggacatc cggaaaaata
      121 gaagatctct atccgcacgg cgccttccta tgcggccggg acgcgctgat ctcctgaagg
      181 acagggcagc aatcccgctg catgggaggt ttgcgcaggg cggcgccgca ttgccgcaac
      241 gcttccgaca aatcggtttc gagtttctgc aaatccgaga ttctggcccg caccacggcg
      301 agttgtgcgg cgatcaaatc ccggacctgg gcgcaggcct caccgggatg gcgttgaatc
      361 gagagtaatt cgcgacactc ttgtcccaaa caggttcttg gactctgaaa tagaggggag
      421 cgccctccta taatcgacgt tgacgaggtc gtttctttgc cggaagcccc tcgccgaacg
      481 ctgcccacag gagggcaatc caatcctaca agtcgcgagc ctgttcaatc aactgctcca
      541 gcacttcccc cgcaccgagt tcgccgctct cgtgaagaag catgccgccg agcgtgccgc
      601 taaggcaccg ttaagaaatg aactatccag ctagacgccg tggggatgga tttcgtagtt
      661 ccagtcgccg tggaacttgt cccgcctcag acgcaccccc gcaagttcgt gatcctcgat
      721 cttggttccg gctggatagc tgccagagtc cagttcagca taaaccttca gtccctttgc
      781 cgtggtggtg gcagcgatga gattgacgat gacttcgtag ctcagaagtg gctttccgcg
      841 ccagttctga ctgatgaagc tgaacagtct gtgctcgatc ttgttccact tgctggtgcc
      901 cggcggaaag tggctcacgg cgatccgcag ccccgtttcg tccgctagtt tttgtagttc
      961 cactttccaa agccgcagac gcggtccgtt actgcctccg gcatctgcgg tgatcagcaa
     1021 ccgatgggcc ttcggataga ccggctgccc catcgactgc caccaccgcc ggatgctctc
     1081 cacggcgaat gccgcggtgt cgtggtctac tcccacgctc acccatcccg tgttctgggc
     1141 cagatcgtag atcccgtacg gcgtggcgcg ccccagttct ggaatcaaga aatcatgcac
     1201 ccggacatcc tccggcttcc ccttcggccg tagttcccgc ccgctattct taaagtcccc
     1261 gaccagttcc ttcttctttg tgtccaccga gatcacgggt tgcttgctcg caaagaaccg
     1321 ttgcaccttg cgattgatat attcgaattg cgcattccgg tctgggtgtc cgtcgccctc
     1381 agtggtcttg cggttggcct ggagactgta ccccagttcc cgtagcaact cagccaccac
     1441 cgggtagctg atgacatgcc cctgccttcc caattcctcc gccagtcgcc gcacgctctt
     1501 gcaagtccat cgcagcggag attccggatc cccacgcgta accggctcga ccaacttttc
     1561 caggtcgacc agaacggcag cgtcctgctc cgcgatcctc ttacgcccgc cccctggccg
     1621 acgcacgcgt cccaccagcg ccgtcttggg gttcttcaat tccgcgatac cttggcggat
     1681 cacgggacgg gaaattccgg tagcccgcga gacggccgaa atgccgcctc tcggcaacgc
     1741 catgctctcg gcacctgcca ccaaccggcg cgaacgctcg tccagtaacc tgctcagaac
     1801 ggagaagcgc ttcttgattc cggcgaggtc tgtcaccctt ccagaatacg ccgaaacatt
     1861 ctaaattgga aatgttattt cttaacgagc cctaagggct tcacctgctg gacgcagttc
     1921 gtctccatgc tgttctgcca actgggacgc gccgattcct tgcgcgagat ctgcaacggc
     1981 ctggggtgct gcctcgggcg cctggtgcac gtcgggatca cccatgcgcc gcgccgctct
     2041 accctgtcct atgccaacga acaccgtccc gccgccctgt tcgaggaact gttctggacc
     2101 gccgcggcgc gatttcgcaa ccaacaggcc ctcggctcca ggaaacacaa gttccgtttc
     2161 aagaacaaac tcctgtcgct ggattcgaca accatctcgt tgtgcctgac gatgtttccg
     2221 tgggccgact tccgccgcgc caagggcggc gtcaaggctc acgtcctgtt ggaccatgac
     2281 gacttgcccg cttacgtgct gatcaccgaa gcccgacgga gcgacgtcaa actggcggat
     2341 tccttccgtc tcaatgccgg ttccatcgtg gctatcgatc gcgcctatat cgactacgcc
     2401 ctgttcgccc gctggagcat ggccggcgtc ttcttcgtca cccgtatgaa ggacaacgcc
     2461 gccttcgagg tcgttgaaga atgccaactg ccggccaacc gcaacatccg tgtcgatcaa
     2521 ctcatccgcc tcaccgccgc gcaagcccgc gccgactatc cggaactgct gcgacgcatc
     2581 gtcgcctggg atgccgagaa cgaatgcgag atcgtcctgc ttacgaatct gttggagttc
     2641 ggctctacca ccatcgcggc catctacaaa gagcgctgga aaatcgaact gttcttcaag
     2701 gcgctcaagc aaaacctgac cgtcaagacc ttcgtgggca ccagcgaaaa cgccctccgc
     2761 atccagattt ggacagcctt gatcgccctg ctcctgctga agtggctgca ccatctctcc
     2821 aaagccggtt ggtccctgtc caacctggct tcgatgctgc ggctgaacct gttcacgtac
     2881 cgcgacttga cgaaatggct ggctgatccc atggaaacgc c
//
LOCUS       DASTMK010000068         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_579659, whole genome shotgun sequence.
ACCESSION   DASTMK010000068 DASTMK010000000
VERSION     DASTMK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579659"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /gene="topA"
                     /locus_tag="VFQ79_02760"
     CDS             complement(<1..1568)
                     /gene="topA"
                     /locus_tag="VFQ79_02760"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU0138598.1"
                     /translation="MAKALVIVESPAKAKTIGKYLGNQYVVKASLGHVKDLPKKDLAV
                     DIERDFEPRYEIIEGKKKLISELKQAAKTAEAVYLAADPDREGEAICFHLSEELNGNR
                     KKAGSSIYRVMFNEITANAIRKAFERPGQVNLNLVNAQQARRVLDRLVGYKISPLLWD
                     KVRRGLSAGRVQTVALRLIVEREHEIRGFQKREYWTIDVGLGAKKPPILNARLARRND
                     SAVEIANENAAATIVSALDGVDFRVQSVVTKEKKRNPVPPFITSTIQQEASRKLRFSV
                     KRTMALAQRLYEGLELGSEGAVGLITYMRTDSTRVSGEALAETRSFIGERYGASYLPS
                     SPNVYRSKKDIQDAHEAIRPTSVLRTPESLENVLAEDELKLYRLIWKRYVASQMTPAV
                     YDQTTIEVNAQGRDGARYLFRATGSVPKFDGFLAVYEEGKDQSDEEDEELKHKLPAVT
                     EGEVLKFKSISPEQHFTEPPPRYTEATLLSAMEGAGKLVEDDELREAMEAKGLGTPAT
                     RAAVIEGLLYEGYI"
     gene            complement(1585..2820)
                     /gene="dprA"
                     /locus_tag="VFQ79_02765"
     CDS             complement(1585..2820)
                     /gene="dprA"
                     /locus_tag="VFQ79_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU0138599.1"
                     /translation="MDRRTRLADPMATQQFTREEELHWLALKLIPGLGTRKAGQLIER
                     FRTPQAVFRSSISELEAAGASPGVARNISSGCSFEDAVLQQERMAASGALAIPISDPR
                     YPPMLREIFDPPILLFARGRVELLNRVMIGVVGTRRPSTYGMAVADRLSGDLSTAGLV
                     IASGMARGIDTSAHRAALRAGGDTVAVFGCGVDVLYPSENRKLSNDIAEKGLLLSEFP
                     MGAPAFPQNFPVRNRIISGMSVGVLVVEGAQYSGSSITARLALDQGRDVFAVPGNITS
                     KMSWGPNLLIKQGAKLVQDWNDVVVELAPDVRLRLMQETQGRLGEEGDTDATGESVQA
                     SLPLGPMAEIGRRVLAELKVDSPVPIDNLLETLENCSSSELIATLFELEMLGMIRQLP
                     GKNFVKIWQDGVFGSSHSR"
     gene            2947..>3221
                     /locus_tag="VFQ79_02770"
     CDS             2947..>3221
                     /locus_tag="VFQ79_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEU0138600.1"
                     /translation="MTYKTVIALGALAAGLLGAQSDWPVYGYDPGASRFSPLKQINAG
                     NVHRLQRAWTFHTGKPGSEGIPIVVDGVMYLVGANGLFAVEPETGKQ"
BASE COUNT          649 a          984 c          878 g          710 t
ORIGIN      
        1 tggatatagc cctcgtagag caggccctcg atcactgccg cacgcgtggc cggggtgccc
       61 agccccttgg cttccatcgc ctcgcgcagc tcgtcgtcct cgaccagctt gcccgcgcct
      121 tccatcgccg agagcagcgt cgcttcggta tagcgcggcg gcggttccgt gaagtgctgt
      181 tcaggggaaa tcgacttgaa cttcagcact tcaccctcgg taacggcagg cagcttatgc
      241 ttcagttctt cgtcttcttc gtcgctctgg tcctttcctt cctcgtacac ggcaaggaaa
      301 ccgtcgaact tcggcacgga tccggtcgcg cggaacaggt atcgagcgcc atcccttccc
      361 tgtgcgttca cttcaatcgt tgtctggtcg tagacggcgg gcgtcatctg ggaggcgaca
      421 tacctcttcc aaatgaggcg gtagagtttc aactcatctt ccgccagaac attctccaga
      481 gattccggag tccgcaacac ggacgttggc cggatcgctt cgtgggcgtc ctgaatgtcc
      541 ttcttgctgc gatagacgtt gggcgaagac ggaagatagg acgctccata gcgttcgccg
      601 ataaagctgc gagtctccgc gagcgcttca cccgatacac gcgtggaatc cgtacgcatg
      661 taggtgatca ggccgaccgc accctcgcta cccaattcga gaccttcgta gagccgttgc
      721 gccagcgcca tcgtgcgctt gacgctgaag cgcaattttc gggatgcctc ctgctggata
      781 gtggacgtga taaacggagg aacagggttg cgctttttct cttttgtgac gacggattgc
      841 accctgaaat cgactccgtc cagcgcggag acaatcgtcg cggcggcatt ttcgttggca
      901 atctccaccg ccgagtcgtt ccgcctggcc agccgcgcat tcagaatggg gggcttcttc
      961 gcgcccaggc caacatcaat ggtccagtat tcgcgtttct ggaagccgcg gatctcgtgt
     1021 tcacgctcga caatcagccg tagcgcgacg gtttgcacac ggcccgcgga caaaccgcgg
     1081 cgcactttat cccacaacag cggtgagatt ttgtatccca ccagccggtc gagcacacgc
     1141 cgcgcctgtt gcgcgttcac gagattcaga tttacctggc ctggccgctc gaatgctttg
     1201 cggatagcat tggcggtgat ctcgttgaac attacccggt agatgctgga acccgccttc
     1261 ttcctgttgc cgttgagttc ttctgagaga tgaaagcaaa tagcttcacc ctcgcggtcg
     1321 gggtcggccg ccaggtatac cgcctcggcg gtcttggcgg cctgcttcaa ttcggatatg
     1381 agctttttct tgccttcaat gatttcatac ctgggctcga agtcgcgttc gatgtcgacc
     1441 gccagatctt ttttgggaag gtctttgacg tggccgagcg acgcctttac aacgtactgg
     1501 ttacccaaat acttcccgat tgttttcgcc ttcgccggcg attcgacgat gacgagagct
     1561 tttgccataa aattgaatta ctttctaccg tgagtgggac gaaccgaaaa cgccatcctg
     1621 ccagattttg acaaagtttt ttccgggcaa ctgccggatc atgcccagca tctccagctc
     1681 gaacagcgta gcgattaact ccgacgagga acaattctcc aaggtctcaa ggagattgtc
     1741 aatcggcacc ggactatcca ccttcaattc agcgagtacg cgacggccga tttcagccat
     1801 gggtccgaga ggtaatgacg cttgcacgga ctctccggtt gcatccgtgt ccccctcctc
     1861 tcccagacgc ccctgagttt cctgcatcag ccggagccgc acatccgggg ctagttccac
     1921 aactacgtca ttccagtcct gcacgagctt agccccctgc tttattaaca ggttagggcc
     1981 ccaactcatt tttgaagtta tattgccggg caccgcaaaa acatcacgac cctgatccag
     2041 agccagcctg gcggtaatgg aagagccgct gtactgagcg ccttccacta ccaggacacc
     2101 gacgctcata ccgcttatga tgcgattcct taccggaaag ttttgaggaa aagcgggagc
     2161 gcccatcggg aactccgaca gcagtagtcc tttctccgct atgtcgttcg ataatttccg
     2221 gttttccgaa ggatagagga catccacgcc gcacccgaac acggccactg tatctccacc
     2281 cgctcgaagc gcggcgcgat gggcggaggt atcgatacca cgcgccatcc cgctggcaat
     2341 caccaggccg gcggtgctca ggtcacccga gagccggtcc gctacagcca ttccatatgt
     2401 gctgggcctt cgcgttccca ccacgcctat catcacccgg ttcagaagtt ccaccctgcc
     2461 gcgcgcgaac agcaggatgg ggggatcgaa gatctcccgc agcatcggag ggtagcgggg
     2521 atccgaaatc gggatcgcca gggcgcccga tgcggccatg cgctcctgct gcaaaacagc
     2581 atcctcaaag gaacaaccgc tcgaaatatt ccgtgccaca ccggggctgg ctccggccgc
     2641 ttcaagttcg gaaatagatg atcggaagac tgcttgcggg gtacgaaacc gctctatgag
     2701 ctgtcctgct ttcctggtgc ccagccccgg gatcagcttc aaggccagcc agtgaagctc
     2761 ttcttcgcgg gtaaactgtt gcgttgccat cggatcggcg aggcgtgtcc gccggtccat
     2821 ccgcttatga atgtaacaga cggaggagtt tcccgcaacc cggcacagca cacctcagag
     2881 atacttggtg cgcgaaagga gccgtccctc tcaatgtcgt gcgtaccccc tatactggtt
     2941 aaaaagatga catacaagac cgttatagcc ctgggtgctc ttgccgccgg tcttctggga
     3001 gcccaaagcg attggccggt atatgggtac gatccgggtg ccagccgctt ctcaccgctc
     3061 aagcagatca atgcagggaa cgttcatcgg ctccagcgcg catggacatt tcatacgggc
     3121 aaacccggct cagagggaat tccaatcgta gtggacggcg tgatgtacct ggtcggggca
     3181 aacggtcttt ttgccgtgga acccgaaacc ggaaagcaga t
//
LOCUS       DASTMK010000069         9097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_547282, whole genome shotgun sequence.
ACCESSION   DASTMK010000069 DASTMK010000000
VERSION     DASTMK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9097
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547282"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VFQ79_02775"
     CDS             <1..1268
                     /locus_tag="VFQ79_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEU0138601.1"
                     /translation="EMPAITARICGICPISHLLASVKACDEIMAVRVPPTAAKLRELL
                     HCGQFIQSHALSFFHLSAPDLLLGMDSDPARRNVLGLLDKYPDIARDGIALRKFGQCV
                     IQRLAKERVHPSWTVPGGVNAPLEPGAREEMLKELPGAKAIARRTLALFKSVLDEFQE
                     EIENFGTDPTMYAGLVDRRGRLQLYDGNIRFLDSEGTVLTEVDTPASYSDYIGEASLP
                     DSYLKAPYYKPAGYPGGVYRVGPLARLNVADSCGTPEADHEFGEYHQRFGRVVQSSFQ
                     YHYARLIEALYAIERAEILLSDPSILGKHVRAKAGVNALEGVGIAEAPRGILIHHYRV
                     DEKGAIRWANLIIATGHNNLAMNRSIEQVSRHFVDGSRLKEGMLNRVSAVVRAYDPCL
                     SCSTHALGQVPLRIQLIAADGTVADELRT"
     gene            complement(1278..3608)
                     /locus_tag="VFQ79_02780"
     CDS             complement(1278..3608)
                     /locus_tag="VFQ79_02780"
                     /inference="COORDINATES: protein motif:HMM:NF022436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEU0138602.1"
                     /translation="MNSFLKYLQWDGIYRTSGSLNHNPVLDVVPIVPVEEVEKDRSEA
                     LVRKGPHEEAKLLNTVDKPVIGVRIRKVDAQTRPFEEALAIRIKLYESSTNLIRSLAL
                     RIYRSYDPHTDAGDVHASKLVYQECLNRAQIDQLPDEDPTVGKGGKKPNPKEGPPLNP
                     MSLLDLTMGALKPALMKPPERKRDSDLYGYGNGSIHVADSPYKVVVWVSLRESKFSEW
                     KPGTAPQALEVTRNTRRDQSSSRHKKIPAPPADTIGVNLTAAAPLPLIRFAAVTPCTG
                     SEATTGRFLALRYKDELHLDELNLKKLDPLKTFAGKKRKDVWRHPEKPFVAYLQNCSY
                     FPNGAVSSFQGDFWVDGRWQSQMRFLFEQMERLYSNDATLKAAPLAVVLAPEWYFSRC
                     ILNGEEADPNVFPPYTYDEVQQIIGTLCDASKAYPNALLIPGTILWATPLNMKKHPKR
                     YAVINTAVAVKNGGLVHLYHKKSHGGDDRATTQFVLAPPNTPEGVTREEAPYLSEEIR
                     NAVKEASTSFFYCDGVSFAFEICADHGQAVGQARREYVRRFPDGRGADVHIHVGYASQ
                     TIVETTTVPRQGGFHLDCDGQQSVVRSPATQTIVNTRVITQRSGSIANGGAALVKCRE
                     MVGIDGFRLPDPLPVATAPPENPADGDSFLIGPNTTGAWAGQESKIATWNAKDGVWTF
                     DDLADNPAVSDLLNNVLVDYNLRDEFAQRFDRTDVKLGRAAVQKLKNDLTPAVDVVIS
                     QPGERPTCLAVYKSPFDLNVQIPPASPNRQPVHGPQ"
     gene            complement(3618..4250)
                     /locus_tag="VFQ79_02785"
     CDS             complement(3618..4250)
                     /locus_tag="VFQ79_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEU0138603.1"
                     /translation="MPQHTVSQGECMVSIADRYGFFWQTLWNHPQNAALKQSRKDASA
                     LYPGDIVFVPEKRIKRITRPTAKRHRFRVKGVPAKLRLRILEGGEPRAGEPYSIVIDG
                     VTRSGVIGAGGFVEIPIAPGAQTGKLIVGHPDEAEEYDLKLGCLDPLDEITGLQARLG
                     NIGFYDGPVDGMESEELRSAVKLFQGSVGLRPTGELDAGTREALASEHDI"
     gene            complement(4264..4950)
                     /locus_tag="VFQ79_02790"
     CDS             complement(4264..4950)
                     /locus_tag="VFQ79_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138604.1"
                     /translation="MSGWQSSTSEGPRFRRIWRFGRTGIEVIRRADLAAGERFFEDGR
                     LFISALGRILSDPFERRTALRLYESIASRLPAGIANAETGRVRSAMEAALDAAFRSGL
                     LVILMRAERVVELPVLRREAEGWHTTAQGAAEAKPHARAMSAGSGIADAGSDEDTVTT
                     QVKTWVEVQLVDMAGNPMKSARYTIKLPDGSLQEGFLSQEGIARCDNIDPGMCEISFP
                     DLDAEAWEPA"
     gene            complement(5002..5589)
                     /locus_tag="VFQ79_02795"
     CDS             complement(5002..5589)
                     /locus_tag="VFQ79_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU0138605.1"
                     /translation="MKRLFWAGVVGVVVCLAFYLAYLTIRIQRQSVVDESRPADVIVV
                     LGAAEYSGRPSPVLQGRLDHALELYRRNMAPRLLTTGGAGGDPVFTESTVGRDYLIKR
                     GVPAESIIVEPEGESTVHSTAAVSEIMRRMGLHSAIVVSDGYHIYRVKKMLEAAGLKV
                     YGSPRPSPMQGDPSLWWLYFRQAVGYLLWSIGIPI"
     gene            5694..6485
                     /locus_tag="VFQ79_02800"
     CDS             5694..6485
                     /locus_tag="VFQ79_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0138606.1"
                     /translation="MPKAAVTSAKNPVLKEVRRAIVRGSLTGDGLCVAEGFHLLEEAL
                     RTDCRVDVVLAAESVRTAVEGHVRALPSIRILVLPDDLFRAVSATESTQGVIALVEPK
                     KWSLEHLFRGRAMVAVLDGVQDPGNAGAIIRAAEAFGATGLVFLKGTVSPFNPKALRA
                     SAGSIFRVPLISGLDQDLARAAFEQKRLDVYAAVPDGGRTVDEVDLKRRFALIIGSEA
                     RGVSPRLRGAAFDLRIPTLGVESLNAAVAAGILLYEARRQRRLDQ"
     gene            6482..7807
                     /locus_tag="VFQ79_02805"
     CDS             6482..7807
                     /locus_tag="VFQ79_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU0138607.1"
                     /translation="MSLFEGLPQPRGDAAKRPLAERMRPETLEEFVGQEHILGPGKPL
                     RAQIERDQLSSLILWGPPGVGKTTLAQIIARLTKCDFIPFSAVLSGIKEIKAVMADAE
                     RSRRSGRRTVVFVDEIHRFNKAQQDAFLPYVERGDIILIGATTENPSFEVISALLSRT
                     KVYSLRALTPEEIVDLLNRALRDRQRGLGSLELTASEELLRQIAVYSSGDARTAYNVL
                     ELAAAASDTPEISTQAVEDALQRKVLLYDKSGEEHYNLISALHKSVRSSDPDAALYWL
                     ARMLEAGEDRMYIARRMVRMAIEDIGLADPRAVEQAIAVMQTVHFLGIPEGDLALAQL
                     AIYLSVAPKSDAAYRALTAVTKDVTNSVAEPVPLHLRNAPTRQMKQWGYGAGYEHAHN
                     LADAITSMECLPPGMSGRQWYFPTNRGVEARIAERLEAIRAGKKGKPDS"
     gene            7972..8307
                     /locus_tag="VFQ79_02810"
     CDS             7972..8307
                     /locus_tag="VFQ79_02810"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0138608.1"
                     /translation="MTVETYTSERRSSGRFPIEREVRFRVLSKRSNEEPGTGRTVNIS
                     SSGVLFTTSRELIPGRKLELSISWPVQLNNECPLKFVARGRVVRSDHGWAAIEIQQYE
                     FRTAGKISA"
     gene            8634..>9097
                     /locus_tag="VFQ79_02815"
     CDS             8634..>9097
                     /locus_tag="VFQ79_02815"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0138609.1"
                     /translation="MQKFRFVLRIGVTLACLLALSLSLPGQERFGNVSGVASDATGAV
                     LPDVAVTITNKATNRTYNTRTGNDGSYQAQDLEPGRYSVRFEKSGFARYEVADALVLV
                     GRTAKVDAQMQVGTVEVTVQVTETAPLIDPTSTLVANNITAEEFNSLPKARS"
BASE COUNT         1803 a         2770 c         2702 g         1822 t
ORIGIN      
        1 atgaaatgcc ggcgattacc gcgcgcattt gcggcatctg tcccatcagc catctgctgg
       61 cctccgtaaa ggcatgcgat gagattatgg ccgtgcgggt tccgccaaca gcagccaaat
      121 tgcgtgaact gctacactgc ggccagttca tacagtcgca cgccctgagt ttcttccatc
      181 tctccgcacc ggatctgctc cttgggatgg attccgatcc cgcccggcgt aacgtcctcg
      241 gcctgctcga caagtacccc gatatcgcga gggacggcat cgcgctacgc aagttcggcc
      301 agtgtgtgat acaacgcctt gccaaagaac gcgtccatcc gtcctggacg gtgcctggag
      361 gcgtcaatgc cccgctcgag ccgggcgcac gcgaggagat gttgaaggaa ctaccgggcg
      421 cgaaggcgat cgcccgccgg actctcgctc tgttcaagag cgtcctcgac gagtttcagg
      481 aggaaataga aaacttcggc acggacccga cgatgtatgc gggcctggtg gaccgtagag
      541 gccgcctgca actctacgat ggcaacatcc ggttcctgga ttccgaaggg acggtgctca
      601 cggaggtcga caccccggct tcttactccg attacatcgg cgaggcttcc ctgcccgact
      661 cctacctgaa agctccctac tacaagccgg ccggatatcc gggcggagtg tatcgagtcg
      721 gcccgttggc aaggttgaat gtcgcggatt cctgcggcac gcccgaggcg gaccacgagt
      781 tcggcgaata tcaccagagg ttcgggcgcg tggtccagag ttcgttccag tatcactatg
      841 cccggttgat cgaggcgctt tacgccatcg agagggctga gatacttctg agcgatccgt
      901 ccattctcgg aaagcacgtc agggcgaaag cgggagtgaa cgccttggag ggtgtaggca
      961 tcgccgaagc gccgcgaggc atcctcatcc atcattaccg cgtcgacgaa aaaggtgcga
     1021 tccggtgggc gaaccttatt atcgcgaccg gacacaataa cctggcgatg aaccgcagca
     1081 tcgagcaggt gagccggcat tttgtggacg gctcgcggct gaaagaaggc atgctcaacc
     1141 gggtatcggc cgtcgttcgc gcttatgacc cgtgcctgag ctgttccact catgcactcg
     1201 gtcaggtgcc tctacgcatt caactgatcg cggcggacgg tacggtagcg gacgaattgc
     1261 ggacgtaagg cgcgccctta ctgcggaccg tgcaccggct gccgattggg cgacgcgggc
     1321 gggatctgga cgttgaggtc gaacggactc ttgtagacgg cgaggcaggt gggccgctcg
     1381 cccggttggc ttatgacgac atcgacagcc ggcgtaagat cgttcttcag cttctgcacg
     1441 gccgccctgc cgagcttcac gtcagtgcga tcaaaccgct gcgcgaactc atcccgcaaa
     1501 ttgtaatcga ccagtacgtt attcaagaga tcgctcacgg ccgggttatc ggccaaatcg
     1561 tcgaacgtcc acacaccgtc cttcgcgttc caggtggcga tcttcgactc ctgcccggcc
     1621 caggctccgg tagtattagg gccgatcaga aaagaatctc cgtcggccgg gttctccgga
     1681 ggcgctgtcg ccaccggcag cggatcgggc aggcggaatc cgtcgattcc aaccatttcc
     1741 ctgcacttaa ccagagccgc acccccgttt gcgatggagc cgcttcgctg cgtaatgact
     1801 cgcgtgttga caatcgtctg cgttgcaggt gaacgaacga cagattgctg gccatcgcag
     1861 tccaggtgga atccgccctg ccttggcacg gtggtcgtct ccacgatcgt ttgcgacgca
     1921 taccccacat gaatgtgcac gtcggcgccg cgcccgtccg ggaagcgccg gacatattcc
     1981 cgccgcgcct ggccgacggc ctgtccgtgg tccgcgcaga tctcgaaggc gaagctgact
     2041 ccgtcgcagt agaaaaaact ggtcgaggct tctttgacgg cattacggat ttcttcggac
     2101 aggtacggcg cttcctccct agtcaccccc tcgggagtgt tggggggtgc cagcacgaat
     2161 tgcgtcgtag cgcgatcgtc tccgccgtgc gatttcttgt gatacaggtg gaccaagccg
     2221 ccgttcttca cggccactgc cgtgttgata acggcgtacc gcttgggatg ctttttcata
     2281 ttcagcggcg tggcccaaag gatggtgccc ggtatcagca gtgcgttagg gtacgcctta
     2341 cttgcatcgc acagcgtgcc gataatttgt tgaacctcgt cgtaggtgta aggcggaaaa
     2401 acgttcgggt cggcttcctc accgttcaga atgcagcggg aaaaatacca ctccggcgcc
     2461 agaacgacag cgagaggcgc ggctttgaga gtcgcgtcgt tcgagtacag gcgctccatc
     2521 tgttcgaata gaaatctcat ttgcgactgc cacctcccat ctacccagaa atcgccctgg
     2581 aagcttgaca ctgccccatt cggaaaatag ctgcagttct gcaaatacgc gacaaagggc
     2641 ttttccggat gccgccaaac gtccttcctc ttcttcccgg cgaaggtttt gagcgggtcc
     2701 agttttttta aattcagctc gtcgagatga agctcgtctt tatacctcag cgccagaaag
     2761 cggcctgtgg tcgcttcgct gccggtgcag ggtgttaccg ccgcgaatcg gatgagcggc
     2821 agcggcgcgg cggctgtcag gttcactcca atggtgtccg ccggcggtgc tggaatcttc
     2881 ttgtgtctgg aagaagactg gtcccggcgc gtattgcgtg taacctccag cgcctgcgga
     2941 gcggtgcccg gcttccattc gctgaacttg ctctcgcgca gcgagaccca cacaacgact
     3001 ttatagggcg aatccgcaac atggatcgat ccgttcccgt agccgtacag atcgctatcc
     3061 cgcttgcgtt ccggcggctt catcagggcc ggtttcaacg cgcccatcgt gagatccaac
     3121 aaggacatcg ggttgagtgg cgggccttct ttcgggtttg gcttcttgcc tcctttcccc
     3181 accgtcgggt cttcatccgg aagctggtcg atttgagccc gattcaaaca ctcctgatac
     3241 acaagtttcg atgcatgcac gtcgccggcg tcggtgtgcg gatcgtagct gcgataaatc
     3301 cgcaacgcca ggctccgaat cagattggtc gaggactcat acagtttgat gcgaatagcc
     3361 agtgcttcct caaacggcct ggtctgcgcg tccaccttcc gaatgcggac tccgatgacc
     3421 ggcttgtcaa cggtgttcag caactttgcc tcttcatggg gccctttccg gaccagcgct
     3481 tcgctcctgt ctttctccac ctcttcgacg ggaacgattg gaaccacatc cagaaccgga
     3541 ttgtgattca gcgatccgct cgtccggtag atgccatccc attgcaggta tttcagaaag
     3601 ctgttcattc gtccgcttca aatgtcgtgt tcgctcgcca gtgcctcacg cgtgccggcg
     3661 tcgagctctc cggttgggcg aagacctacc gagccttgaa agagcttcac cgcgcttcga
     3721 agctcttcgc tttccatacc gtcgaccggt ccatcgtaga aaccgatatt gccgagcctg
     3781 gcctggagtc ccgtaatctc gtcgaggggg tccaggcagc cgagcttgag atcgtattcc
     3841 tccgcctcgt ccggatggcc tacgatgagt ttcccggttt gcgcgcccgg agcgatagga
     3901 atttcaacaa agccgccggc gccgatgaca ccgctgcgcg tgacgccgtc gatgacaatg
     3961 gaatacggct cacccgcccg cggttcgccg ccttcaagaa ttcgcaaacg cagtttggcc
     4021 ggaacgcctt tcacgcgaaa gcgatgccgt tttgctgtgg gccgcgtgat ccgtttgatg
     4081 cgcttttccg gaacgaacac gatatcgccg ggataaagcg cgctggcgtc ttttcgcgat
     4141 tgcttcagcg cggcgttctg cggatggttc cacagagttt gccaaaagaa tccgtaacgg
     4201 tcggcgatgc tcaccatgca ctcgccctgc gataccgtgt gctgcggcat attcatcctt
     4261 tactcaggcc ggctcccagg cttccgcatc caggtccggg aaactgattt cacacatgcc
     4321 tggatcgatg ttgtcgcacc gggcgatacc ctcctggctg aggaaaccct cctggagact
     4381 tccgtccgga agcttaatgg tgtatctcgc actcttcatc gggttcccgg ccatgtccac
     4441 cagttgcact tcgacccacg tcttcacctg ggtggtaaca gtgtcctcat cggaaccggc
     4501 atcggcgatc ccgcttccgg cgctcatggc gcgggcgtgc ggctttgctt ccgccgcgcc
     4561 ttgcgccgtg gtgtgccagc cttccgcctc gcgccggagt accgggagtt ccacgacgcg
     4621 ttccgcccgc atcagaataa cgaggaggcc cgagcgaaac gccgcatcga gtgcagcttc
     4681 catcgcggac cgcactctgc cggtctccgc attcgcgatt cccgccggca ggcgcgaggc
     4741 aatcgactcg tagaggcgca aagcggtgcg gcgttcgaat gggtccgaca ggatgcggcc
     4801 gagggcgctg atgaacagac ggccgtcttc gaaaaagcgt tcaccggcgg ccaggtcggc
     4861 gcggcgaatc acctcgatgc cggtgcgtcc gaatcgccag atccggcgaa accgcggtcc
     4921 ctcggacgtt gaggattgcc agcccgacat ttcttgaatc ccagtgaaaa ggtggagagt
     4981 attatatcga accggccggt tctatattgg gatgccgatg gaccagagca gataaccgac
     5041 cgcctgacgg aaataaagcc accacaagct cgggtctccc tgcatcggcg aaggacgcgg
     5101 cgagccgtag accttcaacc ccgccgcctc cagcatcttc ttcacccgat agatgtggta
     5161 tccatcgctg acaacgatgg ccgagtgaag ccccatgcgg cgcatgattt cgctcactgc
     5221 cgccgtcgag tgtaccgtgc tctcgccttc cggctccaca ataatggatt cggccggaac
     5281 gccgcgtttg atcaggtaat cgcgcccgac tgtgctctcg gtgaacaccg gatcgccgcc
     5341 ggcgccaccg gtagtcaaca gccgcggagc catattccgg cggtacaatt ccagcgcgtg
     5401 gtccagtctt ccctgcaaca ccggagatgg ccgtccgcta tattccgctg cgcccaaaac
     5461 gacaatcacg tccgccggac gcgattcatc gacgacagac tgccgctgga tccggatcgt
     5521 gagataggcc aggtagaacg cgaggcacac caccacgcca actacgccgg cccagaaaag
     5581 gcgcttcatg ctgtgagcga tacaattgtc gaattgagca atccacctac agtctacatc
     5641 ggagcgcccc ggcgtccgaa cgggccggct tgtggccaag gagacggtct gaaatgccca
     5701 aagcggcggt gaccagcgcg aaaaatcccg tcctcaagga ggtccgccgg gcgatcgttc
     5761 gcggcagcct caccggcgac ggactgtgcg ttgccgaagg cttccacctg cttgaagaag
     5821 ctttgcggac cgattgccga gtggatgtcg tgttggcggc cgaatcggtc cgcacagcgg
     5881 tggaaggtca tgtccgggcg ttgccttcca tacgcatcct ggtgcttccc gacgatctgt
     5941 tccgggctgt atcggcgacg gaatcgaccc agggtgtcat tgcgctcgtg gaaccgaaaa
     6001 aatggagcct ggagcatctg tttcgagggc gggccatggt agccgttctg gatggagtgc
     6061 aggaccccgg caatgccggt gccattatcc gcgctgcgga ggcatttggc gctaccggac
     6121 tggtgtttct gaaaggcacg gtcagtccgt tcaaccccaa ggcgctccgg gcatcggccg
     6181 gctccatctt tcgcgtgccg cttatctccg ggctcgatca ggatctcgca cgggccgcct
     6241 tcgagcagaa gcggctcgac gtctacgcgg cagtgccgga cgggggccgt acggtggatg
     6301 aggtggacct caagcggcgc ttcgcgctca tcatcggcag cgaagcgcgc ggcgtcagcc
     6361 cccggctacg gggggccgcc ttcgatctgc gcattccaac gctgggagtg gaatcgctga
     6421 acgccgccgt cgcggccggc atcctattat atgaagcacg ccggcagagg agactcgacc
     6481 aatgagcctg tttgaaggtt tgccacaacc gcgcggcgac gccgcaaagc gccccctcgc
     6541 cgagcgtatg cgtcctgaaa cgctcgagga gttcgtaggg caggaacaca tcctcggacc
     6601 cggcaaaccc ctccgggcgc aaatcgagcg cgaccagttg agttccctca tcctgtgggg
     6661 gccgccagga gttggcaaga ccactctggc gcaaatcatc gccaggctga cgaaatgcga
     6721 cttcatcccg ttcagcgccg ttctcagcgg catcaaagag atcaaggccg tgatggcgga
     6781 cgccgaacgc agccgccgct ccggccgccg cacggtcgtg ttcgtcgacg aaatccaccg
     6841 ttttaacaag gcgcaacagg acgcctttct gccctatgtg gagcgcggag acattatcct
     6901 gatcggcgcc accacggaaa acccgtcgtt cgaggtgatc tccgcgctgc tttcgcgcac
     6961 gaaggtgtat tcgctgcgcg cgctgacgcc ggaggagatc gtcgatctgc tcaaccgggc
     7021 gctccgcgat cgccaacgcg gcttgggctc gctcgaactg accgcgtcgg aggaattgtt
     7081 gcggcagatc gctgtatatt cgagcggcga cgcgcggacc gcttacaacg tgctcgaatt
     7141 ggccgcggcg gcgtcggata ccccggagat ctcgactcag gctgtggagg atgcgctaca
     7201 gcgaaaggtc ctgctttacg acaagtcggg ggaagagcac tataacctga tttccgcgct
     7261 ccacaagtcg gttcgttcca gcgatccgga tgcggcgctc tactggctcg ccagaatgct
     7321 cgaagccggt gaggatagga tgtatattgc acgccgcatg gttcggatgg cgatagaaga
     7381 tatcggcctg gcggacccgc gcgccgtcga gcaggccatc gcggtgatgc agacggtgca
     7441 cttcctgggc attcccgagg gcgacctcgc cctggcgcaa ctggcgatct atctatctgt
     7501 agcgcccaag tcggatgcgg cttaccgcgc gttgaccgcg gttacaaaag acgtgacgaa
     7561 ttcggtcgct gagccggttc cgcttcacct gcgaaatgcg cccacacgtc agatgaagca
     7621 gtgggggtac ggcgccggct acgaacacgc gcacaatttg gcggacgcga tcacctcgat
     7681 ggaatgcctg cctccgggga tgagcggaag gcaatggtat ttccccacga accgaggcgt
     7741 ggaggcgcgc attgcggaac gcctggaagc catccgggcc ggaaagaagg gcaagccgga
     7801 ttcttgaaaa ccagtctccc agtacttaag aaccgctacg atatatccaa cggcctattg
     7861 tttggacctg ccggaacagg caagctgaac atgagttaac tgagccgcgg gcgccagcag
     7921 gcagcggcat tttgcggacc cggaacacgg gacctttgtt cggaggaagc agtgacagtt
     7981 gagacctaca catcggagcg gagaagttcc ggccgctttc ccatcgagcg tgaagttcgg
     8041 ttccgtgtcc tgagcaagag gagcaacgaa gaacccggca caggcagaac ggtcaacatc
     8101 agcagttcag gtgtgctctt caccacgagc cgggagctca ttcccggcag gaagctggag
     8161 ctttccatta gctggccggt gcagctcaac aatgagtgtc cgctgaagtt cgtcgcccgg
     8221 ggacgggtgg tccggtccga tcacggatgg gccgccattg agattcagca atacgagttt
     8281 cgcacggctg ggaagatttc cgcctaaccc cctcgtcgcg gcagaacgcg cactttttcg
     8341 tgcgcggctt cacccatgcg tagattcacc ctcccctatc taagtggccg gagagccgct
     8401 tttacggtaa acgccagagt gctttaccgg aaacgctgct ccggcaggaa aaatggatca
     8461 gggattccat tttcaagatt ttcttatagt acacgcgctc tgaattttca aatctcatac
     8521 ctcaccgaca cgagcagata ggaaacgaca accacttacc ggtggaacaa atcgtggaat
     8581 cccaattgct agagaagtgc atcagcaatt cgcacaatcc ccgtgaggag tatatgcaaa
     8641 aatttagatt cgtattgagg attggagtca ccctagcctg cctgctggcc ttgagccttt
     8701 cgctgccggg ccaggagcga ttcggcaacg tttccggtgt ggcatcggac gccaccggag
     8761 cggtcttgcc agacgtggcg gttacgatta cgaacaaggc gacaaaccgc acgtacaaca
     8821 ccagaactgg caatgatggc tcctaccagg cgcaggattt ggagcctgga cgctattcgg
     8881 tgcggtttga aaagtccggt ttcgcccggt atgaagttgc cgacgccctt gtactggtgg
     8941 gcagaacagc gaaagtcgac gcacagatgc aggtcggcac ggtggaagtg acggtgcaag
     9001 tcaccgaaac agctccgctg atcgacccca ccagcacgct ggtagcgaac aacataaccg
     9061 ctgaggaatt taacagcctt cctaaagcca gaagttt
//
LOCUS       DASTMK010000070         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_163604, whole genome shotgun sequence.
ACCESSION   DASTMK010000070 DASTMK010000000
VERSION     DASTMK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163604"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFQ79_02820"
     CDS             <1..875
                     /locus_tag="VFQ79_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0138610.1"
                     /translation="GRSLVTKKTHTVGVVVTTVADPFVAEVVAGIEEVVQKRGYAVFL
                     ANSNAIPEREIDVVRSFQGRRVDGVLVMASRVGALYRPLLAELNVPIVLIDNQHPGEF
                     AHSICIEDRPGARLAVNHLVGLGHRRIAYIGNQFGMQVDRHRRAGYRDVLKKERIAPS
                     PDLEVLGDGTAPGGKAAMGQLLGLREPPTAVFCFNDLSAIGAVRALHDRGLKIPGDVS
                     VIGFDDLPIVSYVEPPLTTIRQPKVEMGKRAARMLLGLLDGETVESDVRVPGELIVRS
                     STAHPRGRRLSRKS"
     gene            complement(937..2421)
                     /locus_tag="VFQ79_02825"
     CDS             complement(937..2421)
                     /locus_tag="VFQ79_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0138611.1"
                     /translation="MATRRAFLKTAAGAAASAPAVLRGASRPLREKPNLLFIWTDEQR
                     HDTLAISGNSRFRTPALNRLASESIVFRRNYVTQPVCTPSRGSILTGLYPHQHKSVSN
                     NIPIARDTKTQPELLNDSAYRSGYFGKWHLGDEVFAQHGFEEWVSIEDLYSAHFGKNR
                     NSAERSSYHAYLLSQGYKPDNAEENKFSRQFAVRLPIHDCKPAYLARHASRFILENRN
                     DPWLLHVNFLEPHMPFHGPLNDLHSEEEAPVPANYPGDAVDHEPELYRKSRQRYLENG
                     FEGHDLKTRAGWQRLNRNYAGLCSQVDDALSRILWTLEASGQSDNTVVVFTSDHGEMM
                     GSHTLVGKSVFYEEATRVPLLIRAPFLHRRQIVVDRPTGSIDIVPTVLDLLGAKPAAE
                     LPGQSLVPFLEGKPLHEDHVFSEWYTPDNGPAGRCVISPEGEKLVLFDKDHNMFFDTR
                     RDPLELSNLYYKDQPRDAVLRLRQKLDKWQERTGDTIQFSGREI"
     gene            complement(2434..3678)
                     /locus_tag="VFQ79_02830"
     CDS             complement(2434..3678)
                     /locus_tag="VFQ79_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138612.1"
                     /translation="MGFPYESDYNNLAPRAGFAWDVFGDGHTAIRGHYGISYDALTAE
                     QASGVLIPQPFGLSYTLNNPAALSAPYAGTTNPFPFIFDPASARFVLPVRIPKAVDGG
                     VRNPYAQTFGLNVQRQFASLLVDVGYVGTLGRKLIILREVNPAIPGPGATTANTNARR
                     IYAPAFESIGGLFSDGRSNYHSLQVQVVRRWTAGLTFNANYTWAKSIDEAVSGGNAFA
                     TVDQGGAQNPFNRRADRALSDFDIRHRLAASFLYEIPFRDRGGWSRLYGGWELGGLVT
                     AETGSPFTVVSGRDNSLTAVGFDRPDVIGDPRLEAGSRQESIQRYFNTEVFRRNADGQ
                     FGNAGRNILTGPSRFMLDTSLTKRFAIAEHHSLQLRGDAFNILNRPNFSDPVASLASS
                     NFGQITSAGAGRILQVSLKYSF"
BASE COUNT          772 a         1135 c         1123 g          761 t
ORIGIN      
        1 tcggacgaag cctggtaaca aaaaagactc acacggttgg tgttgttgtt accaccgtgg
       61 ccgacccttt cgtcgcggag gtcgtggccg gcattgaaga agtagttcag aagcgcggct
      121 acgccgtatt cctagccaac agcaatgcaa taccggagcg ggagatcgat gtggtccgtt
      181 cttttcaagg gcgccgagtc gacggcgtgc tggtgatggc ttcccgcgtc ggcgcgctct
      241 accggccact gcttgccgag ttgaacgtgc caatcgtgct gatcgacaac caacaccccg
      301 gcgaatttgc ccactcgatc tgcatcgaag accggcccgg cgcgcgtctg gcggtaaacc
      361 atctggtggg cttggggcac cgtcggatcg cgtatatcgg aaatcagttt ggaatgcagg
      421 tcgaccgtca ccgccgtgcc ggctatcgcg atgtcctgaa aaaggagcgc atcgctcctt
      481 cgccggatct tgaagtactg ggagacggaa cagcccccgg cggcaaagcg gcaatgggtc
      541 aattacttgg attgcgtgaa ccacctacgg cggtattctg tttcaacgac ctgtcggcta
      601 tcggagcggt gcgagccttg catgaccgcg gcctgaagat accgggggac gtctccgtca
      661 tcgggtttga cgacctgcca atcgtttcct acgttgaacc ccctctaacg acgatccggc
      721 agcccaaggt ggaaatgggg aagcgggcag ccaggatgct gctcggccta ttggacggag
      781 agacggtgga atcggatgta agagtgcccg gcgaattgat tgttcgttcc tccacagcac
      841 acccgcgggg gcgccgtctt tcaagaaaaa gttagacctc tggccggaac atcacagtag
      901 ccgcggctgt aaacagcacc cgtgtggaaa cgcgcatcag atctctctcc cgctgaactg
      961 aatcgtgtcc cctgtgcgct cctgccactt gtctaacttt tgtcgcagac gcagcaccgc
     1021 gtctcgcggt tgatctttgt agtacaggtt gctcagctcc agcggatccc ggcgtgtatc
     1081 gaagaacatg ttgtggtctt tatcgaaaag caccaacttt tctccctcgg gagagatcac
     1141 acacctgccc gccggaccgt tgtcgggcgt gtaccattcg gagaacacgt gatcctcgtg
     1201 caacggcttg ccctccagaa acggaacaag gctctgtccg ggcaactccg ccgcgggctt
     1261 cgcgccgagc aggtcgagta cggttggcac aatgtcaatg gaacccgtgg gccggtccac
     1321 gacgatctgg cgccggtgca gaaacggcgc gcgaatcagc agcggaacgc gagttgcttc
     1381 ctcgtagaac accgacttgc ccaccagcgt atgactcccc atcatctcgc cgtgatcgct
     1441 ggtgaagacc accaccgtgt tgtcggactg gccggacgct tccagagtcc acaggatccg
     1501 gctcagcgca tcgtccacct gcgaacacag ccctgcgtag ttgcgattga ggcgttgcca
     1561 tccggcgcgc gtcttcaggt catggccctc aaagccattc tccaggtagc gttgccggga
     1621 cttgcggtac aactcgggct cgtggtccac cgcgtctccg gggtagttgg cgggaaccgg
     1681 agcttcttcc tcgctgtgaa gatcattcaa aggcccgtga aagggcatat gcggctcgag
     1741 aaagttcaca tgcaacagcc acggatcatt ccgattctcc aggatgaagc gggatgcatg
     1801 ccgcgccagg tacgccggtt tgcaatcgtg gatcggaagc cgtacggcga actggcggct
     1861 gaacttgttc tcttcggcat tatcgggttt gtaaccctgg ctcaaaagat aagcgtggta
     1921 tgaactgcgc tcggcggagt tgcggttctt tccgaaatgg gccgagtaca gatcttctat
     1981 ggatacccac tcctcgaagc cgtgctgggc gaacacctcg tcgccgagat gccatttccc
     2041 gaaatacccg cttcgatacg cggaatcgtt caacagttcc ggctgcgtct tcgtgtcgcg
     2101 cgctatcggt atgttgttgc tgacggactt gtgctgatgc ggatacaggc ccgtcagaat
     2161 cgacccgcgc gacggagtgc ataccggctg ggtaacgtag ttgcgccgga agacaatgct
     2221 ctccgaagcg agccgattga gcgcgggcgt gcgaaaccgc gagttacccg atatggcgag
     2281 cgtatcgtgc cgttgttcgt ccgtccagat aaagagcaga ttcggttttt cgcggagcgg
     2341 gcgcgaagcc ccgcgcaaga ccgccggagc gctggccgcc gcaccggccg cggttttcag
     2401 gaatgctctt cgtgttgcca tcgtcgattc ctttcagaag ctgtacttca gcgagacctg
     2461 caaaatacgg cccgctcccg ccgacgtgat ctgcccgaaa ttgctcgatg ccagcgaggc
     2521 gacaggatcg ctgaagttgg ggcggttcag aatattaaaa gcatcacccc gcaactgtaa
     2581 cgagtgatgt tccgcgattg caaagcgttt ggtgagcgaa gtatccagca tgaaccggct
     2641 ggggccggtc aggatgttgc gtccggcatt cccaaactgg ccgtccgcat tgcgccggaa
     2701 cacctccgtg ttgaagtagc gctggatgga ctcctgacgg ctgcccgctt ccaggcgagg
     2761 gtcgccaatc acgtccggcc tgtcaaagcc aaccgccgtc agggagttgt cgcgtccgct
     2821 gaccaccgtg aaaggactgc ccgtctcagc cgtcaccaaa cctcccagct cccatccgcc
     2881 atacagacgc gaccagccgc cgcggtcccg gaatgggatc tcgtatagga acgatgccgc
     2941 caaacgatgc cggatgtcaa aatcgctgag cgcgcggtct gccctgcggt taaacggatt
     3001 ctgcgcgccg ccctggtcca cggtggcgaa ggcatttccg cccgataccg cttcgtcgat
     3061 cgatttcgcc caggtgtagt tggcattgaa tgtcaaaccg gccgtccatc ggcgcactac
     3121 ctgcacctgc aagctgtgat agttggagcg tccgtcggaa aaaaggcctc cgatgctctc
     3181 gaatgccggc gcataaatcc gtcgggcatt cgtattcgcc gtcgtcgctc caggacctgg
     3241 aatcgcggga ttcacttccc gcaggataat cagcttgcgt cccagcgtgc cgacgtatcc
     3301 aacatcgacg agcagagaag caaactgccg ttgaacattc aacccgaagg tctgcgcata
     3361 cggattgcgg actcctccgt cgacggcctt gggaatacgc accggcaaca cgaaacgagc
     3421 cgacgccggg tcaaaaataa acgggaaggg gtttgttgtg ccggcgtaag gggccgataa
     3481 ggccgcggga ttattcagcg tgtagctcaa cccgaacggc tgtggtatca ggacaccgct
     3541 cgcctgttcc gcggtgagcg catcgtaaga aatcccatag tggcccctga tggcggtgtg
     3601 gccgtctccg aaaacgtccc atgcaaaccc ggcgcgcggt gcgagattgt tgtagtcgct
     3661 ctcgtaagga aaacccatcg tcgttctgat aaacaagacc ggccggagca cccggcaaca
     3721 cctgcgactg cactcctgga acaaagccga cggcgatgcc tcgcttctct ttccagcgcg
     3781 ggaacagttc c
//
LOCUS       DASTMK010000071        14686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_451576, whole genome shotgun sequence.
ACCESSION   DASTMK010000071 DASTMK010000000
VERSION     DASTMK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451576"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..765
                     /locus_tag="VFQ79_02835"
     CDS             76..765
                     /locus_tag="VFQ79_02835"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEU0138613.1"
                     /translation="MGNLVVATASIVLLVSGQDGFTESRERMVKHQIQARGVRDAQVL
                     KAMRDTPRHLFVPEKLRSRAYEDTPLPIGFGQTISQPYIVAAMTELLDVEPEHRVLEI
                     GTGSGYQAAVLSPLVREVFSMEIVEDLAKSAAVTLREAGCSNVSVRYGDGYKGWPEKA
                     PFDRIILTAAPPEIPQALFDQLAPGGKLVAPVGSSPLNQELVVVDKGRDGKLKRRTVF
                     PVRFVPMVTNN"
     gene            782..1744
                     /gene="ligD"
                     /locus_tag="VFQ79_02840"
     CDS             782..1744
                     /gene="ligD"
                     /locus_tag="VFQ79_02840"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU0138614.1"
                     /translation="MPARKQVLKVRGRQVPVTNLDKIFYPAAGFTKGQVIDYYIRVSR
                     YLLPHLKQRPVTMKRYPDGVQGEFFFEKNAPRFTPEWVKTFPVPRRTGKADIRYILIN
                     DLPTLVWCENLASLELHPFLHKVPHLDRPRTVVFDLDPGEGTGVLECARAAFLLKEVL
                     DELGLQAFPKVSGSKGIQVYVPVNTLVTYDMTQPFAHTVARYLESDHKDFIIAEMAKE
                     ARRGKVFIDWSQNAGHKTTVGVYSLRAKHDRPFVSLPVEWEELGHAMKKKDSDSLYFD
                     PESALARLEERGDLFAPVLKLKQKLPRDLTARFPVKTSFRPTGS"
     gene            complement(1790..3121)
                     /locus_tag="VFQ79_02845"
     CDS             complement(1790..3121)
                     /locus_tag="VFQ79_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0138615.1"
                     /translation="MALRLRTVTGVLALLIAGGVSAQAQKYDLLLKGGHVIDPKNKLS
                     AMRDVAIAGGRIALVAEKIDASEAFKSVDVSGLYVTPGLLDIHAHVYTGTGEPHSYAG
                     DNSVYPDGFTFRVGVTTVADAGGAGWRNFSDFKERVIDRSRTRVLSFLNIVGHGMRGG
                     KYEQNLEDMEAKPAADMALSHKDLIVGIKTAHYAGPEWTPVENAVKAGTIANIPVMVD
                     FGSNKPERPISVLLTEKLRPGDIYTHVYSGLRDEQLESGLINPALLEGRKRGVIFDVG
                     HGGGSFLWRVAVPLLKGGFLPDSISTDLHIGSMNTGMKDMLNVMDKFLAMGMKLDDVI
                     LRSTWNPAREIKREELGHLSPGALADVAVLRLQKGNFGFIDMYGARMNGSQKLLCELT
                     LRDGKIVYDLNGIARPDWTTLPKDYRATGDPKWDAYAPRGRRPRPAGQTAR"
     gene            complement(3125..4726)
                     /locus_tag="VFQ79_02850"
     CDS             complement(3125..4726)
                     /locus_tag="VFQ79_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138616.1"
                     /translation="MYGFLRERLGRRDLFRGGALAAFAGLFRSSAGASAPAAGLKLGS
                     DLYQSIGVRPLINARGTFTIISGSLMLPEVRAAMVEASQHHVHIDELMDAIGARLAEL
                     TKAEWGMVSCGCAAALTHATAACIAGGNPDLHVRIPNLSGFPKDEVIIPKHSRNVYDA
                     AVRCVGAKILEVGTAEELEAAFGPKTAMIYILAGPQADSGPLSTRAIADAAKRRNVPV
                     LVDAAAEILTVPNVHLENGATLVAYSGGKCIRGPQTAGLLLGRKSLVQAAWVHSSPHH
                     GFARSMKVGKEEAMGMLMAVEMWMKRDHKAEWNQWMARLDHVAKRLSGIDGVTTSVRE
                     PNGLSNRTPSLVISWDSARLGISGRDVAQHLLNTEPRIALPTGGGGRGMGGGTQTGVS
                     VVPYQMTAGDEKIVADRLYATLKNPPKREDPPVRPPAADLTGRWNVHIDYLASGSDHV
                     LHLKQQDGRIEGTHQGDFVSRDLGGSISGDEVRLFSAYTERHGDSLMFQFTGKVAGDG
                     ISGTLDMGEYLQARWTAKRHEFRRR"
     gene            complement(4753..5679)
                     /locus_tag="VFQ79_02855"
     CDS             complement(4753..5679)
                     /locus_tag="VFQ79_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138617.1"
                     /translation="MTLLCFALLPVLFWDQGPETADQLKAAGITRVAVPSSMESAWRK
                     RLGVEVRGVDPAGAIPLPKPGVEFRVNEASATRSPWIDLNGWQFLRNPKGHFYYEAPG
                     PSAALAAAEAFAYGADAMVHTDAAGLEPLGHMLAFLERVGDAKLPPVADIGFIDDGSD
                     EAGEVMNLMMRRNLLFRVVTAPDPSLPLHVEIGSPKFPKEEAADPSEMAQKIRQALGD
                     SARSVRLFGSEVVIARLTGNGNRLRIELLNYGGGRRAASVRIRVRGRYTKQELRAFRL
                     PDARLADFVSSGDATEFTLPELEYYAVVDLTR"
     gene            complement(5676..6791)
                     /locus_tag="VFQ79_02860"
     CDS             complement(5676..6791)
                     /locus_tag="VFQ79_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0138618.1"
                     /translation="MNRRDFLYIAGAVPLVSATQEIPKYHIVSKFKPSSNPGMPGRYR
                     GQVVNVHAESSIDAASGKVNAEVVRAMLARGMQALTGDKDDRDAWARFITSKDVVGIK
                     VNCSGAPNIMSSPEIVAGIVERLMAVGVPARQIYIYERFLGQMRSVSYEQYVPEGVQI
                     TAAEQARGSILGYDPNTYVDVDFFGEDDTRSNLVRLVAERLTKIVNVPNLKEHQASGV
                     TGCLKNIAYGDFSNVARSHRSEKTNTYSFIGTLASVEPLRSITVLNIMDGLRAVWHAG
                     PFSMDPRFRFFPKRMMFGTDPVAMDRLVIDVVEEKRKAEGAPSIWDRSPERVKNVRRP
                     DKSVNAFIREPGHVEYASKLGLGEYDVGKIRRKDIEL"
     gene            complement(6809..7069)
                     /locus_tag="VFQ79_02865"
     CDS             complement(6809..7069)
                     /locus_tag="VFQ79_02865"
                     /inference="COORDINATES: protein motif:HMM:NF022535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEU0138619.1"
                     /translation="MNWNDAAEELLNNILLRTPRPVRETTEQDIRRIAEQMAEEESKQ
                     RVGVETVIAAWIETTPETLRSALPQQMESFGLDPSEYEHLLG"
     gene            complement(7091..8569)
                     /locus_tag="VFQ79_02870"
     CDS             complement(7091..8569)
                     /locus_tag="VFQ79_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEU0138620.1"
                     /translation="MWQEYHEATRHSVESLRRSQHVLDWNNMPDPFRHYEGVPVLDLP
                     ADPPPPPVPALQVLSGIKGQVETSEGAAFLSQLLFYSAAISAAKIVPSTGYKYALRVN
                     PSSGNLHPTEFHFVTRGLKNWPDGLYHYRVSSHMAEQRATGLFEIPGEQNTAPLLFVL
                     TSIAWREAWKYGNRAYRYCLLDIGHAWQALATAALAMGCQAHAFGCFPDDEVSSRCGF
                     AQDEWPMLLIELRGAIPAGEPDTKTPLWLGGEPNRLSEKTRSYPLIDSIHTATKRLGE
                     MPVRGDPPAGVVPSGGTRLPEPAPAPTAFGHVARRRRSALDFIGGESSMTLSELSAVL
                     QAASGSLAGDFSSGSFLRMYLYVHRVEGLEPGVYRYWPGESALERIRSGDQRVAAAGL
                     SLGQDLAGNACVAFSITGDLERATRLHGDRGYRYVHFEAGAVGHRMYLAAEALGLGAT
                     GIGAFFDERVHHYLDLETGEDQAVYHFAIGHPMPDPRLSAGD"
     gene            8695..10074
                     /locus_tag="VFQ79_02875"
     CDS             8695..10074
                     /locus_tag="VFQ79_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0138621.1"
                     /translation="MGRGCCLLIFFLGTVGLIPGATEETPSQDADVVFRSDVSLVRVD
                     AQVLDRNNRAITGLHAEDFILREEGQPQQIRNFESEEMPVDVLLLLDVSGSMRPHVER
                     IASAAHQALAVLGSGDRVAIMVFDRSTRIRLPFRANRQEVQSGLDAVVREESFNGGTD
                     ITRALMDASRYVAREARREARRAIVILTDDRTERGRDEAGVSRALAEADAVLSALLAP
                     DALRGRSGYPGPHGGGTWPGGGSAGGPLGDIILGRRRGPYGGGGRTGPVILGPQTASA
                     GTAEIARSSGGDAMSVDDAEAFETTLARMRQRYALHFYLPDGTHPGQERNITVELASA
                     AGRRYPGAEVRYRRTYLSPGGAGTSDPVLISHVPDNPQAGPADDEPPRLQRRKGVSEA
                     STRSARGPITADSERTGNPRANAQDVPAPREGWRRADEAATAADTPPPAQPDAKPGRW
                     RKLEPGEEP"
     gene            complement(10085..12376)
                     /locus_tag="VFQ79_02880"
     CDS             complement(10085..12376)
                     /locus_tag="VFQ79_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138622.1"
                     /translation="MSPSTIRLPASRINPVSQNIQERFYPLPNFGDPNVLTSQNYREN
                     KIRPRDPSTYWTTRIDHRFSDRDNIFGRYTWHRAFNRPYEGNLPTIGRRFQQRDNRAA
                     TVSYTHTFSATLLNEFRWGVVLNNNPVEGPINGPSLVNELGLVGLAPDLPDISGILRV
                     MWAGVGLEGITQPNYTRPGFRNYLHDFQDHVSWFRGRHNFKVGFNLSRIEWDDMSANA
                     NLFGTLTFSNTFTSAGRAGQGHPYADFLLGIPTSAARAFPPIRVDRLRWQYDFFVVDD
                     FKVNNKLTLNLGLRYELHPGWREENDRISMFDIGSGRIVVPEGSQSKVSPLFPEGYVG
                     IVNAGELGLPNRTLVRTDRNNFAPRVGLAYRPWGNRTVFRAGYGFFFDVVPRNLNMGG
                     IPYVLNEPTYTNPANNPDVIFPRVFPATGSAGPSTVGIPAAVNPNLLMPYSMQYNVTI
                     EHQRWGTGFRASYIGTNTRKGDYNYNYNAPVPDDRPFVDKPRPFPQYPGISYFTNGAG
                     HQYNGLTVEAERSFSHGLYFQTSWTWARDVFDLERGQALENPFDRSREVAVSQDIPTH
                     RYNLNFIYELPFGRGRTFLSGASRLTNLIVGGWEVSGIYSYFSGQFLTPLWTGPDPTG
                     TAFTNSRTRANVEIRPDHLQNANLPSDQRSVNQWFDPSAFDAPNAGSFGTSAKGVIKG
                     PDVNVWHTGFHKNFIFTEKMRLRLEMTATNFFNHPNWSNPAVNISQVANVGVISGVGG
                     VNGASTGDQPGARSFRLGVRFEW"
     gene            complement(12291..13358)
                     /locus_tag="VFQ79_02885"
     CDS             complement(12291..13358)
                     /locus_tag="VFQ79_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0138623.1"
                     /translation="MKTHSFASSITVFALLFSSAAHAQTTFATITGTAVDATGAVIAN
                     ARVTATNVDTGIKTEAFTNDVGNYIIPQLNQGRYELRAQATGFKEFVAQDITLTSRDV
                     RRVDIRFEVGSVDTVVEVSAGATLIETETARISDTKDSMQLNTLPLNTRGLWAFLALS
                     PNVLQAATNSTIRFAGSRGNQSHWAIDGTTMSDGVDETQIGPLANYIESFQELKIDMS
                     NNTAEFGTIGQVTIISKSGTNEFHGSLFDYYSTPWFRARNPFSPSRGTGISHTPGGSA
                     GGPVYLPKLYDGRNKTFFFFSFETSRGSPVQQLLNPTVPPQPWRSGDFSGLGVTINDP
                     LTGQPDQSRFAKYPGAVLSAS"
     gene            complement(13398..14648)
                     /locus_tag="VFQ79_02890"
     CDS             complement(13398..14648)
                     /locus_tag="VFQ79_02890"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEU0138624.1"
                     /translation="MKTSQRYSAAEVERMMKLQDVLLKAMAKRITWWEAAEIIGVTDR
                     TMRRWRERLEEDGYSGLADRRKGKPSDKRVRLEVAGQVLKLYQETYRDFNIRHFHEKL
                     KEVHGIELSYTWVQKALQGAGLVARRGRRGKHRRRREPRPMTGMLLHIDGSKHRWFCD
                     ERRYDLIVILDDATKEIYYAQLVEEESTRTVMAALREVIERKGIFCALYSDRGSHFFV
                     TPKAGEKVDKHRLTQIGRAMKELGVQMVPAYSPQARGRGERSFGTWQGRLPQELRVAG
                     ITTVEEANRFLRERYIAEFNGKFTVAAKEKGTAFRRSTRSDLNWVFTLQTERVVEKDN
                     TVAIGERRWQLEKTQKDLQVVFTDADRSRVNETGQIDKLPTGCYLAVIGPCSTTPLRA
                     PPTDRFYRHFALTRLWLNAIVQIK"
BASE COUNT         2800 a         4544 c         4378 g         2964 t
ORIGIN      
        1 cagaactgcg cgttttttct ggtgtttgcg cggcgcccgc tggttcttga cactccaacg
       61 gcctaccatt gaggtatggg gaacctcgtg gtggcaacgg catcgattgt tctcctggtg
      121 agcggccagg acggcttcac cgaaagcagg gagcgtatgg tcaaacatca gatacaggct
      181 cggggcgtca gagatgcgca ggtgttgaag gcgatgcggg acacgccgcg gcatctattc
      241 gttccggaga aactgcgttc gcgcgcttat gaagacaccc cgctgccgat cggcttcggc
      301 cagacgattt cacagcctta tatcgttgcc gcaatgacgg agttgctgga cgtcgagcct
      361 gagcaccggg tgctcgaaat tgggacgggg tccggctacc aggcggctgt tctttccccc
      421 ctcgtgaggg aagtctttag catggagatc gtggaggacc tcgcgaaatc ggctgccgtc
      481 acgctgcgag aggccggctg ttcgaatgtg tccgtccgct atggcgacgg gtacaaggga
      541 tggccggaga aggctccgtt cgaccgcatt atcctgaccg ccgcgccgcc cgagattcca
      601 caggccctgt tcgaccagct cgcacccggc gggaagctcg tcgctcccgt cggcagctct
      661 cctttgaacc aggagcttgt cgttgtggat aaggggcggg acgggaagct gaagcgccgg
      721 acggtgtttc cggtgcgttt tgttccgatg gtaacgaaca actaagagcc gttgcctgcc
      781 gatgccagcc cgtaaacagg tgttgaaggt tcgaggccgg caggtcccgg tgacaaacct
      841 ggataagatc ttctatcccg ccgccggttt caccaagggg caggtcatcg actactacat
      901 ccgggtctcg cgctatctcc tgccgcatct gaagcaacgg ccggtgacga tgaagcgata
      961 cccggacggt gttcagggcg agtttttctt tgaaaagaat gcacccagat tcacgccgga
     1021 atgggtgaag accttccctg tgccgagacg aaccggcaaa gcggatatcc ggtacattct
     1081 gattaacgac ctcccgactc tcgtctggtg cgagaatctc gccagtctcg aattacatcc
     1141 atttctgcac aaggttccgc atctcgacag gcccaggacg gtggtgttcg atctcgaccc
     1201 tggggagggc acgggcgtgc tcgaatgcgc aagggcggcc tttctgctga aggaagtgct
     1261 cgatgagctc gggcttcagg cctttccaaa ggtgtcgggc tcaaaaggca ttcaggtgta
     1321 tgttccggtc aacactctcg tgacctacga catgacacaa ccgtttgccc acacggtagc
     1381 gcggtatctg gagagtgacc acaaggactt catcatcgcg gagatggcga aagaggcgcg
     1441 ccgcggtaag gtgtttatcg actggagtca gaacgccggc cacaagacta ccgttggcgt
     1501 ttattctcta cgggcgaaac acgaccggcc gtttgtttcg cttcccgtag agtgggaaga
     1561 gttaggccat gcgatgaaga agaaagattc ggattcgctg tacttcgatc ccgagtccgc
     1621 gctggcgcgg cttgaagagc gcggcgacct gtttgctccg gtactgaagc tgaagcagaa
     1681 gttgccccgc gacctcacag cccggtttcc agtgaaaacc tcgttccgtc cgaccggatc
     1741 gtgatcgcta cggagattcc cgcgaagttg cgcctcgcag ggacagcact cagcgcgcgg
     1801 tctggcccgc cggacgcggc cttcgtccac gcggcgcgta ggcgtcccac ttcggatctc
     1861 cggtggcccg gtagtcttta ggaagcgtgg tccaatccgg ccgcgcgatt ccgttcaggt
     1921 cgtaaacgat ctttccgtcc cgaagcgtga gctcacagag cagtttctgc gatccgttca
     1981 tgcgcgcgcc gtacatgtcg ataaatccga aatttccctt ttggagacgc agcacggcga
     2041 catcggcaag agcaccgggc gataggtggc ccagctcttc gcgcttgatc tcgcgagcgg
     2101 gattccacgt cgagcggaga atgacgtcat ccagcttcat gcccatggcg aggaacttgt
     2161 ccatgacgtt cagcatgtcc ttcatgcccg tgttcatgct cccgatgtgc aagtcggtgg
     2221 agatggaatc cggcagaaat ccgcccttta gcagtggaac cgcgacgcgc cataagaagc
     2281 tgccgccgcc gtgccccacg tcgaaaataa cccctctctt ccggccctcg agcagagcgg
     2341 gattgatcag gccggactcc agttgttcgt cgcgcaagcc ggagtagacg tgcgtgtaaa
     2401 tgtcgccggg ccggagtttt tccgtcaata aaacgctgat gggccgttcc ggcttgttcg
     2461 atccgaaatc gaccatcacc gggatgtttg cgatggtacc ggcctttacg gcgttttcca
     2521 ccggcgtcca ctcggggccg gcgtagtgcg cggtcttgat gccgacgatc agatctttgt
     2581 ggctgagggc catgtcagcc gcgggtttcg cctccatgtc ctcaagattc tgctcgtact
     2641 tgccgccgcg catgccgtgg cccacgatat tcaaaaacga gagtacgcgc gttctggagc
     2701 ggtcgatcac ccgttccttg aaatcggaga aattacgcca accggcaccg cccgcatcgg
     2761 ccacggtcgt tacgcccacg cggaaagtga agccatccgg ataaacgctg ttgtctcccg
     2821 cgtaagaatg cggctctccc gttcccgtat aaacgtgggc atgaatatcc agcagtccgg
     2881 gtgtgacgta cagtccagag acatcgacgg acttgaacgc ctccgaagcg tcgatcttct
     2941 ccgccaccag agcgatgcgc ccaccggcga tggcaacgtc ccgcatcgcg ctcaacttgt
     3001 ttttaggatc gattacgtgg ccgcccttga gcagcagatc gtatttctgc gcctgcgcgc
     3061 tcacgccgcc ggcgataagg agcgccagga ctcccgtgac cgttctcagc cttaatgcca
     3121 tctgttatct tctccggaac tcatgtcttt ttgccgtcca ccgcgcctga agatactcgc
     3181 ccatatcgag cgttccggag atcccgtcgc cggcgacttt gcccgtgaac tgaaacatga
     3241 gcgaatcgcc atggcgctcc gtgtaagcgc tgaacaggcg tacttcgtca ccgctgatcg
     3301 atccgcccag gtcgcgcgaa acgaagtcgc cctggtgggt cccctcaata cggccatcct
     3361 gctgtttcag gtgcagaacg tgatcggaac cggaagcgag gtaatcgata tgaacgttcc
     3421 agcggcccgt aagatcggcg gccggcgggc ggacaggcgg gtcttcgcgc ttgggggggt
     3481 tcttgagagt ggcgtacaac cggtccgcga caatcttctc atcgcccgcc gtcatctggt
     3541 acggaacaac cgagacaccg gtttgagttc cgccgcccat gccccggccg ccgccgcccg
     3601 tgggcagcgc gatgcgcggt tccgtattga gcagatgctg ggcgacgtcg cgcccgctga
     3661 tgcccaggcg agcggaatcc caactgataa caagagaggg cgtgcggttg gatagcccgt
     3721 taggctcacg caccgaagtg gtcacgccat cgattcccga tagccgtttc gccacgtgat
     3781 ccagccgcgc catccactgg ttccattcgg ccttgtggtc ccgtttcatc cacatctcga
     3841 ccgccatgag catccccatg gcctcttcct tgccgacttt catcgaacgc gcaaagccgt
     3901 ggtgcggaga actgtggacc catgccgcct gcaccaggct cttcctgccc agcagcagtc
     3961 cggcggtctg cggaccgcga atgcacttgc cgccgctata agccaccagc gtggcaccgt
     4021 tttcgagatg cacgttgggc acggtcagaa tttcggcggc ggcatccacc agcacaggta
     4081 cgttccgtcg tttcgcagcg tcggcaatcg cgcgtgttga cagcggcccg gagtctgcct
     4141 gggggcccgc gaggatatag atcatcgcgg ttttcgggcc gaaggcggct tcaagctctt
     4201 ccgcggttcc cacctcgagg atcttcgcgc ccacacagcg cactgccgca tcgtatacgt
     4261 tgcgggaatg cttcgggata atgacttcgt ccttgggaaa tccgctcaga ttcgggatgc
     4321 gcacatgcag gtcgggattg ccgcccgcga tacaggccgc ggtagcgtgc gtcagcgcgg
     4381 ccgcgcatcc gcaggacacc atgccccatt ccgctttcgt caactccgcg agccgcgccc
     4441 cgatggcgtc catcagttcg tcgatatgga cgtggtgttg agaagcttcg accatcgcgg
     4501 cgcgtacttc aggcagcatg agcgatccgc tgatgatcgt aaaagtacca cgggcgttga
     4561 tgaggggccg tacgccgatg gactggtaaa gatccgagcc taacttcagt ccagcggcgg
     4621 gagcgctcgc tccggcggaa gaacgaaaca agccggcgaa agccgcaagc gccccgcccc
     4681 gaaacaaatc gcggcgcccg aggcgctctc tcagaaagcc atacatgtgc cgtccctcct
     4741 ttgccgcttg tatcagcggg tgagatcgac tactgcataa tactccaact caggcagcgt
     4801 aaactcggtg gcgtcgccgg aggagacgaa gtccgcgagc cgggcgtccg gaagccggaa
     4861 cgcccgcaat tcctgtttgg tataacgtcc gcggacgcga atacggacac tggccgcgcg
     4921 ccgtccgccc ccgtagttca gcaactcgat tcgcagccgg ttaccgttgc ccgtgagccg
     4981 cgcgatcacc acctcactgc cgaaaagcct gactgatcgc gcgctgtctc caagggcctg
     5041 acgaattttc tgcgccatct cactggggtc ggccgcttcc tctttcggga acttcggaga
     5101 accgatttcg acatgcaggg gcagcgatgg gtccggtgcg gtaacaaccc ggaataacag
     5161 gttccggcgc atcatgagat tcatcacctc tcccgcttca tcggatccat catcgatgaa
     5221 accgatgtcg gccacgggcg gcagcttcgc atcgcccaca cgctcgagaa atgcgagcat
     5281 gtggcccaag ggttccagac cggcggcatc ggtgtgtacc atggcgtccg cgccgtaggc
     5341 gaacgcttca gcggccgcca gcgccgcgga cggcccggga gcttcgtaat aaaaatgtcc
     5401 tttcgggttc cgcaggaact gccatccgtt cagatcgatc caggggctgc gcgtggccga
     5461 tgcttcgttc acgcggaact cgacgccggg cttcggcaac gggatggcgc cggccggatc
     5521 aacaccgcgc acctccacgc cgaggcgctt cctccaggct gattccatcg aggatgggac
     5581 cgccacgcgg gtgatgccgg cagccttcag ttgatccgcc gtctcgggac cttggtccca
     5641 gaacagcacc ggcagcagcg caaaacagag cagcgtcata gctcaatatc tttccggcgt
     5701 attttgccca cgtcgtattc tcccaggccc agcttcgatg cgtattccac gtgtccgggt
     5761 tcgcggatga atgcattcac agacttgtcg ggccgccgta cgttttttac cctttcgggc
     5821 gaccggtccc aaatggacgg cgccccctcc gcttttctct tctcctcgac gacgtcgatc
     5881 acaagacgat ccatcgccac cgggtccgtg ccgaacatca ttcgtttggg aaagaagcgg
     5941 aatctcgggt ccatcgagaa cggtcccgcg tgccacacag cacgcaatcc gtccattatg
     6001 tttaaaacag taatggagcg cagcggttct acggaagcca gggttccgat gaaagagtag
     6061 gtattggtct tttccgagcg atgcgaccgg gccacgttcg aaaagtcgcc gtaggcaata
     6121 ttcttaaggc aaccggtgac gccggacgcc tggtgctcct tcaggttcgg gacgttgacg
     6181 atcttggtga gcctttcggc aaccagccgg acgagattgg agcgcgtgtc gtcttcgcca
     6241 aagaaatcca cgtcgacgta ggtgttcgga tcgtagccga ggatggagcc ccgcgcctgc
     6301 tccgccgcgg tgatttgcac cccttccggc acgtactgct catagctcac gcttctcatc
     6361 tgccccagga accgctcata gatatagatc tggcgcgcgg ggacgccaac cgccatcaac
     6421 ctctccacga tgccggcgac gatttccgga gaggacatga tgttgggtgc gcccgagcag
     6481 ttcaccttta tcccgacgac gtccttggag gtgataaagc gcgcccacgc atcgcggtcg
     6541 tccttatcgc cggtcagcgc ctgcatgccg cgcgcaagca tcgcgcgaac tacttcggca
     6601 ttcaccttgc ccgaagccgc gtcgatggag ctttccgcgt gtacgttcac cacctggccg
     6661 cgatagcgcc cgggcatacc ggggtttgac gacggcttga acttcgaaac gatgtggtac
     6721 ttggggattt cctgcgtagc ggacaccagc ggcaccgcac cggcgatgta caagaaatcg
     6781 cgtctgttca tatttttctt ccccagggtc acccgagcaa gtgctcgtat tccgatggat
     6841 ccagaccaaa gctctccatt tgctgcggaa gggcggagcg aagcgtctcg ggtgttgttt
     6901 cgatccacgc agcgatgacc gtctctacgc ctacacgctg cttgctctcc tcttcggcca
     6961 tctgctccgc tatgcgccgg atatcttgtt cagtagtttc ccgcaccggt cgtggggtac
     7021 ggagcaaaat gttgttcagg agttcttccg cggcgtcgtt ccagttcaca gttcgctatc
     7081 ttagcaggac ttagtctcca gccgagagcc gcggatcggg cattgggtgg ccgatcgcga
     7141 aatgatagac ggcctggtct tctccggttt cgaggtcgag atagtggtgc acgcgttcgt
     7201 cgaaaaacgc gccgatgccc gtcgccccaa gtccgagcgc ttcagcggcc agatacatcc
     7261 tgtgaccaac ggcaccggcc tcaaaatgca cgtagcgata cccgcggtcg ccatggaggc
     7321 gggtcgcgcg ctcgaggtcg ccggttattg aaaacgccac gcaggcgttt ccggcgagat
     7381 cctgtcccag gcttagaccg gcagcggcca cacgctggtc accggaccgg atgcgctcga
     7441 gcgccgattc gccgggccag taccgataca cgcccggctc gagaccttcg acgcggtgca
     7501 cataaagata catccggagg aaagacccgg agctgaaatc gccggcgagc gagccgctgg
     7561 cggcctgaag aacggcggac agctcgctga gcgtcatcga cgattctcca ccgatgaaat
     7621 ccagggccga acgccgtcgc cgcgcgacat gtccaaacgc ggtaggggcc ggggctggtt
     7681 ccggcaaccg ggtgccgcct gacgggacta cgccagcggg cgggtcgccg cgcaccggca
     7741 tctcgcccaa ccgcttcgta gctgtatgaa tgctgtcaat gaggggatag gagcgggttt
     7801 tttcagaaag ccggttgggc tcgcctccca accacaacgg cgttttcgtg tccggttcgc
     7861 cggccggaat cgcaccgcgc aattcgatga gcagcatcgg ccattcgtcc tgggcaaagc
     7921 cgcagcggct tgacacttca tcgtcgggga aacagccgaa tgcatgagcc tggcagccca
     7981 tggcgagagc tgccgtggcc aacgcctgcc aggcgtggcc gatgtcaagc aggcaataac
     8041 gatacgcccg gttgccatac ttccaggctt cgcgccacgc gatgctggtc agcacaaaca
     8101 gcagcggcgc ggtattctgt tcgcccggta tttcgaataa tccggttgca cgctgctcgg
     8161 ccatgtggga cgagacgcgg tagtggtaca gcccatcggg ccagttcttg agcccccgcg
     8221 tcacgaagtg gaactccgtg ggatgcaggt ttccggaaga cgggtttacg cgaagcgcat
     8281 atttgtaacc ggtggacgga acgattttgg ccgcgctgat ggcggcggaa tagaacaaca
     8341 gttgagacag aaacgccgca ccctctgagg tttcaacctg ccccttgata ccactcagaa
     8401 cctgtaacgc cgggacagga ggtggaggtg gatcggcggg caaatccagc accggtacac
     8461 cctcgtagtg ccggaacggg tccggcatgt tgttccagtc cagaacgtgc tgcgaccgcc
     8521 gtagggattc gacgctgtgc cgggtcgctt cgtgatactc ctgccacatc actcagctta
     8581 gcaatcctgg gttcgggaag gacttctgaa accagaccgt tcccggacgc gtacacaata
     8641 tcgagcagtg ccttcccgtt cccctgttca ggccggctcg gaaagtgagc gtcgatgggg
     8701 cgtggatgtt gtctcctgat tttcttttta ggcacggtgg gccttatccc cggtgccacg
     8761 gaggaaaccc catcgcagga tgcagacgtc gttttccgca gcgatgtgtc gctggtccgg
     8821 gtagacgcac aggtactcga ccgcaacaac cgcgccatta ccggtcttca cgccgaagac
     8881 ttcattctgc gggaagaagg ccagccgcag cagatccgga acttcgagag cgaagaaatg
     8941 cccgttgacg tcttgctcct gctcgatgtc agcgggagta tgcgtccgca tgtcgagcgg
     9001 attgcatcgg cggcgcacca ggccctggcc gtactcggat ccggggatcg tgtcgccatc
     9061 atggtttttg accgttccac gcgaattcgt cttccgttcc gtgccaaccg gcaggaggtg
     9121 cagagcggcc tggacgctgt ggtacgcgag gaaagcttca acggcggcac cgacattaca
     9181 cgcgctttga tggatgcatc ccgctacgtt gccagagaag cccggcggga ggcgcgccgc
     9241 gccattgtca tccttacgga cgaccggacg gagcgcgggc gcgacgaagc gggcgtctcc
     9301 cgtgcgctgg cggaagcgga cgcggtgctc agcgcgctcc tcgcaccgga tgccctgcgc
     9361 ggccgttccg gttatccggg gccgcacggt ggcggcacgt ggcccggtgg aggttcggct
     9421 ggcggtcctt tgggcgatat cattttgggc cggcgccggg gcccttacgg aggaggcggt
     9481 agaacgggtc ccgtaattct gggaccgcaa accgcttcgg cgggcaccgc ggagattgcg
     9541 cgttcttctg gtggagacgc aatgagcgtc gacgacgccg aagcattcga gactacgctg
     9601 gcccgcatgc ggcagcgata tgccctgcat ttttatttgc ccgatgggac tcatcccggc
     9661 caggaacgca acatcacggt cgaactggcg agcgcggccg ggcggcgcta ccccggtgcg
     9721 gaagtgcgat accggcgcac ctatctttct ccgggtggag ccggcacatc cgatccggtc
     9781 ttgatcagcc atgttccgga caatccgcag gccgggccgg cagatgacga accgccacgt
     9841 ctccagagac ggaaaggcgt cagcgaagcc tcaacccggt cggcgagggg accgataacg
     9901 gcggatagcg aacgcaccgg caacccgcgc gcgaacgctc aggatgtgcc tgcgcctcgc
     9961 gaaggctggc gccgtgccga tgaagccgcg acagcggcgg atactccgcc accggcccag
    10021 ccggatgcca aaccgggccg ctggcggaaa ctggaaccgg gtgaagagcc ttaagcgaag
    10081 ctccttacca ctcgaaacgt acgcccaggc ggaacgacct cgcgcccggc tggtcgcctg
    10141 tcgacgcgcc gtttacgcct ccgacgccgc tgatgacgcc gacattcgcc acctggctga
    10201 tattgaccgc cgggttgctc cagttcggat gattgaagaa attggttgcc gtcatttcca
    10261 atcgcaggcg catcttctcc gtgaagatga agttcttgtg gaacccggta tgccagacgt
    10321 tcacatccgg gcctttgatg acgcccttag ccgacgtgcc gaagctgccc gcattcggag
    10381 cgtcgaacgc ggacggatca aaccactggt tcacgctgcg ctggtctgac ggtagattgg
    10441 cattctgcag atggtcgggc cggatttcca cgttcgcccg tgtcctgctg ttggtgaatg
    10501 ctgtgccagt cggatccgga cccgtccaca gaggtgtgag gaactggccg gagaaatagc
    10561 tgtagatgcc gctgacttcc cacccgccaa caatcaggtt tgtcagccgc gatgcgcccg
    10621 agaggaacgt acggccccgg ccgaacggaa gctcgtagat gaagttgaga ttgtagcggt
    10681 gtgtcgggat atcctgcgat accgccacct cgcgactgcg gtcaaacgga ttctccaacg
    10741 cctggccgcg ttccagatcg aagacgtccc gcgcccaggt ccacgaagtc tggaagtaca
    10801 gcccgtgaga gaagctgcgc tcggcttcga ccgtcagccc gttatattga tgtccggcgc
    10861 cgttggtgaa gtagctgatg ccgggatact gcgggaacgg acgtggcttg tcaacgaagg
    10921 gccggtcatc cggaacgggt gcgttgtagt tgtagttgta gtcgcctttc cgcgtattcg
    10981 tcccgatata ggaggcacgg aaaccggtgc cccaacgctg atgctcgatc gtcacgttgt
    11041 actgcatgct gtaaggcatc aaaaggttgg gattgaccgc tgccggaatg ccgaccgtcg
    11101 aaggacccgc actgccggtg gcggggaata cgcgcggaaa aatcacgtcc ggattgttcg
    11161 ccgggttcgt ataggtcggc tcgttcagga catagggaat cccgcccata ttcaggttgc
    11221 ggggaaccac gtcgaagaag aagccatacc cggcacggaa aacggtcctg ttgccccatg
    11281 gccggtaggc gagcccgaca cgcggcgcga agttgttcct gtctgtccgg accagggtcc
    11341 ggttcggcag tcccaattcg ccggcattca cgatgcccac gtagccctcg gggaacaacg
    11401 ggctgacctt ggactgggag ccttccggca ccacgattct gccggagccg atgtcgaaca
    11461 tcgatatacg gtcgttttct tcgcgccagc ccggatgcaa ctcgtagcgg agtcccaggt
    11521 tcaaggtcaa cttattgttc accttgaaat cgtccacaac aaagaagtca tactgccacc
    11581 ggagccggtc tacgcggatc ggtggaaacg cccgcgcagc cgacgtggga attccgagca
    11641 ggaagtcggc ataaggatgc ccttgtcccg ctcgcccggc gctggtaaat gtattcgaga
    11701 aggtgagcgt gccaaaaagg ttggcgttag cagacatgtc atcccactcg atgcggctga
    11761 gattaaagcc caccttgaaa ttgtggcggc cgcggaacca gcttacgtga tcctggaaat
    11821 cgtgaaggta gttgcgaaaa cccggacggg tgtaattggg ctgtgtgata ccctccagtc
    11881 cgacgccggc ccacatgaca cgcagaatgc cgctgatatc cggcaggtcg ggcgccagac
    11941 ccaccagccc caattcgttg acgagagaag gtccgttgat cggcccttcg accgggttgt
    12001 tgttcagcac gaccccccag cgaaattcat tcagcaaggt tgcgctgaac gtatgggtat
    12061 aggagaccgt ggcggcgcga ttgtcccgct gctgaaagcg gcggccgatg gtcggcaggt
    12121 ttccttcata aggacggttg aaggcgcggt gccacgtata ccgtccgaag atattgtcgc
    12181 gatctgaaaa acggtgatcg attcgcgtgg tccagtaggt ggatggatcc cgcggccgga
    12241 tcttgttttc acgatagttc tgactggtca gtacattcgg gtccccgaaa ttaggaagcg
    12301 gatagaaccg ctcctggata ttttgcgaaa cgggattgat ccggctggcc ggtaagcgga
    12361 tcgttgatgg tgacacctaa ccctgaaaag tcgccactcc gccatggctg tggcggtacc
    12421 gtcgggttca gaagctgctg aaccgggctg ccacgtgatg tttcaaacga aaaaaagaag
    12481 aatgttttgt tgcggccgtc atacagcttc ggcagataga cgggaccgcc ggcgctgccg
    12541 cccggtgtat gggagatgcc tgtgccccgg ctcggtgaaa atggattccg cgcccggaac
    12601 catggcgtgg agtagtagtc gaacagactt ccgtggaatt cgttcgtccc ggatttggag
    12661 ataatcgtca cctggccgat cgtgccgaat tccgcggtgt tgttcgacat gtcgatcttc
    12721 agttcctgga acgattcgat atagttcgcc aaaggtccaa tctgcgtttc gtccacgccg
    12781 tcgctcatcg tggttccgtc aatggcccag tgcgattgat tgccccggct gccggcgaaa
    12841 cgaatcgtgc tgttggtggc cgcctggagc acgttcggcg aaagcgccag gaatgcccat
    12901 aagccacgcg tgttcagcgg cagcgtgttc aactgcatgg aatccttcgt gtcgctgatg
    12961 cgggctgttt cggtttcgat cagcgtggca cccgcgctaa cttccacaac ggtatccaca
    13021 cttcctacct cgaagcggat gtctacgcgg cgcacatcgc ggctggtcaa cgtaatgtcc
    13081 tgtgctacga attccttgaa gccggtggcc tgtgcccgaa gctcgtagcg tccctggttg
    13141 agctgcggaa tgatgtaatt gccaacgtcg ttggtgaacg cttctgtctt aatgcccgtg
    13201 tcaacattcg tcgccgtcac acgcgcgttc gcgataacgg ctccagtggc atcgacagcg
    13261 gttcccgtga tcgtggcgaa ggttgtttgt gcgtgtgccg cgctggaaaa gagcagtgcg
    13321 aacacagtta tcgaggaggc gaacgaatgg gttttcatat tcctccgggt tttgacagaa
    13381 ctacgaacaa ccgaccccta cttgatttga actatagcat tgagccaaag tcgggtcaag
    13441 gcgaaatgcc tgtaaaatct atccgtaggc ggcgcgcgca acggggttgt cgagcacgga
    13501 ccgatcaccg ccaaatagca gcctgtcggt agtttgtcaa tctgtccggt ttcattcaca
    13561 cgtgatctgt ccgcatcagt aaataccacc tgaagatcct tttgtgtctt ctccagttgc
    13621 cagcgccgct cgccaatcgc aaccgtgttg tccttttcaa cgacccgctc ggtttgcagc
    13681 gtgaacaccc agttcaggtc gctgcgcgta cttcgccgga acgccgtccc cttctccttg
    13741 gctgccacgg tgaacttccc gttgaactcc gcaatgtatc gctctcgcag gaatcggttc
    13801 gcctcttcca ccgtcgtgat acccgccacc cgcagttcct gcggcagccg gccttgccac
    13861 gtcccgaagc tccgctcccc gcgcccccgc gcctgcggcg aataggccgg gaccatctgc
    13921 acccccaact ccttcatcgc ccgcccgatc tgcgtcagcc ggtgcttatc caccttctcg
    13981 cccgccttcg gcgtcacgaa gaaatgactc ccacggtcgc tatacagcgc acagaagatc
    14041 cctttgcgct cgatcacctc ccgcagcgcc gccatcaccg tccgcgtcga ttcctcttcc
    14101 accaactgcg cgtagtagat ctccttggtc gcatcgtcca gaatcacgat caggtcgtac
    14161 ctgcgctcgt cgcagaacca gcggtgcttg ctcccgtcga tgtgcagcag catccccgtc
    14221 atcggcctcg gctccctcct cctccgatgc ttgccccgcc ggccccgcct ggccaccagc
    14281 cccgctccct gcagcgcctt ctgcacccac gtgtaactca gctcgatccc atgcacctcc
    14341 ttcaacttct cgtgaaagtg ccgaatgttg aaatcgcgat acgtctcctg gtacaacttc
    14401 agcacctgcc cggccacctc caaccgcacc cgcttgtcgc tcggcttgcc tttgcgccgg
    14461 tccgccagcc ccgaatagcc atcctcttcc agccgctccc gccatcgcct catcgtccgg
    14521 tcggtgactc cgatgatctc ggccgcctcc caccaggtga tcctcttggc catcgccttc
    14581 aacagcacgt cttgtagttt catcatccgc tccacttccg cggccgagta tcgctggctc
    14641 gttttcaccg ctccagcgtc taccgccgct cagctcgaac ggacag
//
LOCUS       DASTMK010000072         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_163659, whole genome shotgun sequence.
ACCESSION   DASTMK010000072 DASTMK010000000
VERSION     DASTMK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163659"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1108)
                     /locus_tag="VFQ79_02895"
     CDS             complement(221..1108)
                     /locus_tag="VFQ79_02895"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU0138625.1"
                     /translation="MPDADAAVAILHAGDNRESVLLIRRVEREGDPWSGHWSFPGGRC
                     DPGDRDLLDTALRELEEECGIRLEREHMETVLPLAVARRRGGRPLQVAPFVFRIDGEL
                     PTALQECEAVESRWVRLSELRDPGRHRFVSVPGRPWEQQFPGIELDPVPLWGFTYRLI
                     TDWLGLVEYSGPLSHAGFETASALLQLLLANGSVLKHGWAERAVLQTWLGQQTVRMAA
                     IEGEIPVPAVLAHFSTPQLQVPALNAIEIRPEFLRLAGLAYEEYWICGHGGLGCGSPL
                     SDKEREPLAATTDETVDLQ"
     gene            1244..2602
                     /locus_tag="VFQ79_02900"
     CDS             1244..2602
                     /locus_tag="VFQ79_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138626.1"
                     /translation="MSDNSLNRRDFFKNTGAMSAALGLAGTALAKPGKMAAGRVLGAN
                     DRINVAAIGVGGRGTYVAKAFAKVGQGAANAQIVGVCDVYQKRVDENKEFHKCDGYLD
                     YREIINRNDVDAVIIATPDHWHAKIALEAMDRGKDVYIEKPMCHTVEEVKQLIDTVKE
                     TKRVLQVGSQTTSGDQWHKAKKAIADGMIGQMIMSQGSYHRNSTEGEWNWPIDEQAGP
                     DAKGENYIDWKMWLGPAPKRAYDADRFFRFRKYWDYSGGVATDLFFHVAAPMNICWGE
                     AQFPYKVMAGGGIYVFHDREVPDTFHLIAEYSKGHSVVLSSSMANSQHIPGLIRGHDA
                     SLIMVEHGRFEGYASHITVKPEKRVYNRELRERSVPPEYGAKWGSAEIKIPVEDRDPM
                     ITHVENFLDCMRTRQKPTLDVETAARAQVLITMSVQSFREGKVLYFDERKFKVVDKPV
                     RA"
     gene            complement(2645..>3833)
                     /gene="aspS"
                     /locus_tag="VFQ79_02905"
     CDS             complement(2645..>3833)
                     /gene="aspS"
                     /locus_tag="VFQ79_02905"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU0138627.1"
                     /translation="QQGSFYALPQSPQLFKQLLMVSGFEKYFQIVRCFRDEDLRADRQ
                     PEFTQVDIEMSFPQQETVFEAIEPLLQRICAVAGYQIEIPFQRLTYAEAMRQYGVDKP
                     DLRLPPFCPVEDLFPNAGLTGEGLPLVAIRIPKTGALSRKERDELKAYGAERGLRVYD
                     DLKRLERDYPEQLAAVRERTGTSEEDLLLVAGWAGAPKGQRPDETVYMACGQLRLFAG
                     QKYNDRHKLLDPKDFRFLWVVDFPMFEWDEEESRWNAAHHPFTSVHDEDLEKLTTDPA
                     HCRAKSYDVVLNGVELGSGSIRIHRRDVQSKVFGALGFSEEEARRRFGFLLEGLEYGA
                     PPHGGIALGLDRLVMILAGENSIREVIPFPKTARGTDLMCDAPSTVPERQLRELGISL
                     RKK"
BASE COUNT          778 a         1155 c         1153 g          747 t
ORIGIN      
        1 ggtgctcgcg gtgcgggcgg gttcggcttc ccggatgccg aaggtggcgc cggtgtcgag
       61 attttttgca tagagcgata cggagccggg aaacgtgctg atccgctcac gaatgcggtt
      121 ttcgagcggc tgagcggcgg cggacaacgc cagagtgaga agcagcagga tgcgcatcgc
      181 ataaataata tcccgccggc gccgcctccg gatggcaaat ttactggaga tccacggttt
      241 catccgtggt ggcggcgagg ggctcgcgtt ctttgtccga tagaggacta ccacagccaa
      301 gcccgccgtg accgcagatc cagtattcct cataggccag accggccagg cgcaggaact
      361 cggggcggat ctcaatcgcg ttcagcgccg gaacctgaag ttgtggcgtg gagaagtgcg
      421 ccagaaccgc cggcaccgga atctcgcctt caatcgctgc cattcgtacg gtctgttgtc
      481 caagccaggt ttgaagcacg gcgcgctccg cccatccatg cttcaacaca gagccgttgg
      541 caagcagtaa ttgcagcaat gccgaggcgg tttcgaatcc ggcatgcgat agggggcccg
      601 aatattccac cagtccgagc caatccgtga tcaggcggta ggtgaacccc catagcggga
      661 ccggatcgag ttcgatgccg gggaactgct gttcccacgg acgtccggga accgatacaa
      721 aacggtgccg tcccgggtcg cgcagttcgc tgagccgcac ccatcgcgac tccaccgctt
      781 cgcactcctg aagagcggtg ggcagttcgc catcgatgcg gaacacgaac ggagctacct
      841 gaagaggccg gccccctctc cgccgggcca ccgccagcgg cagcacggtt tccatgtgct
      901 cgcgctccag ccgtatgccg cattcctctt cgagttcgcg caacgcggtg tcgagcaggt
      961 cgcggtcgcc gggatcgcac cgccctccgg ggaacgacca gtgtccggac catgggtccc
     1021 cctcgcgctc cacgcgccgg atcagcagga ccgattcccg gttgtccccg gcatggagga
     1081 tcgctacggc tgcgtcagcg tcgggcatct ctaattcctt tatacccggg tgctcgaatc
     1141 tcttttgatt cacctgcatg cggggagtac actggaatag gtattttcaa tccgaattct
     1201 gatggtgcgc ctacgtttcc ggctgcatca ggaaggacca ggcatgtcag acaattctct
     1261 caaccgcaga gatttcttta aaaacaccgg cgctatgtcc gccgctcttg gtttagccgg
     1321 aaccgcgctg gcaaagccag gcaaaatggc cgcggggcgg gtacttggcg caaacgatcg
     1381 gatcaacgta gcagccattg gcgtcggcgg gcgcggcact tacgttgcga aagcttttgc
     1441 aaaagtcggc cagggcgcgg ccaacgcgca gatcgtcggc gtctgcgacg tgtatcagaa
     1501 gcgggtcgac gaaaacaaag agttccacaa gtgcgacggt tacctcgatt accgcgagat
     1561 catcaaccgg aacgacgtgg atgccgtcat cattgccaca ccggaccact ggcacgccaa
     1621 gatcgctctg gaggcgatgg accgcggcaa agacgtgtac atcgaaaagc cgatgtgcca
     1681 taccgtcgaa gaggtcaaac agctcatcga taccgtgaag gagacgaagc gtgtcctcca
     1741 ggtgggctcg cagaccactt cgggcgacca gtggcataag gcgaagaagg ccattgccga
     1801 cggaatgatc ggccagatga tcatgagcca gggttcctac caccggaatt caacagaagg
     1861 cgaatggaac tggcccatcg acgaacaggc cggccccgat gccaagggcg agaactatat
     1921 tgattggaag atgtggctcg gcccggcgcc gaagcgcgct tacgacgccg accgcttttt
     1981 ccgcttccgc aagtattggg actattccgg cggtgttgcg acggacctgt tcttccacgt
     2041 cgcggcaccc atgaatatct gttggggcga ggcgcagttc ccctacaagg tgatggccgg
     2101 cggtggaatt tacgtattcc acgatcgcga ggtgccggac accttccacc tgatcgccga
     2161 gtattcgaaa ggccactccg tggtgctgag ttcttcgatg gcgaactcgc agcatatccc
     2221 cggtctcatt cgcggccacg acgcttcgct catcatggtg gagcacggcc ggtttgaggg
     2281 ttatgcatct cacatcactg tgaagcccga gaagcgtgtg tataatcgcg aactccggga
     2341 gcgcagtgtc ccacccgaat atggtgccaa atggggctca gcggagatca agatcccggt
     2401 ggaggatcgc gatccgatga tcacccacgt ggagaatttc ctcgattgca tgcgcacgag
     2461 gcagaagccg acgctggatg tggaaaccgc cgcccgcgct caggtgttga ttacgatgtc
     2521 ggtgcagtct ttccgcgagg gcaaggtgct gtacttcgac gagagaaagt tcaaggtagt
     2581 cgacaagccc gtgagggcct gaacgttttg aggacagggc cggtttttca gccggcccgc
     2641 ctcgtcactt tttccggagc gagattccca attcccgcaa ttgccgttcc ggcactgtcg
     2701 aaggcgcatc gcacatcaaa tccgtgcctc gcgccgtctt cggaaatggg atcacttcgc
     2761 gaatcgaatt ctctcccgcc aggatcatca ccagccggtc caatccgaga gcgatgccgc
     2821 catgaggtgg cgctccatat tcgagccctt ccagcaggaa gccgaagcgg cggcgtgctt
     2881 cctcctcgct gaatccgagt gctccgaata ccttcgactg tacgtcgcgc cggtgaatac
     2941 gaatggagcc cgatccgagt tcgaccccgt tcagcacgac gtcgtaggac tttgcgcggc
     3001 agtgcgccgg gtcggttgtc aatttctcca ggtcctcgtc atgaaccgaa gtgaagggat
     3061 gatgcgccgc gttccagcgg ctctcctctt cgtcccattc gaacatcgga aagtccacaa
     3121 cccacaggaa gcgaaaatct ttcgggtcca gcagcttgtg ccggtcgtta tacttctgcc
     3181 cggcgaagag gcgcaactgc ccgcaggcca tgtaaacggt ctcgtccggc cgctgtcctt
     3241 tcggcgctcc ggcccaccct gccactaaca acaggtcctc ttcggaggtg ccggttcgtt
     3301 cgcgcaccgc agcaagctgt tccgggtagt cgcgctccag gcgcttgagg tcgtcgtaga
     3361 cacgcaagcc gcgctccgcg ccgtaggcct tcagctcatc gcgctcttta cggctgagag
     3421 caccggtctt tggaatgcga atagcgacca gcggcaatcc ttctcccgtc agaccggcat
     3481 tcggaaacag gtcttcgacc ggacagaacg gcggcaggcg caggtccggc ttatccacgc
     3541 cgtactggcg catcgcttcc gcgtaggtca gacgctggaa gggaatttcg atttgatatc
     3601 cggccacggc gcaaatgcgc tggagcagcg gttcgattgc ttcgaaaacc gtttcctgct
     3661 gcggaaacga catttcgata tcgacttgtg tgaactccgg ctgccggtct gcacgcaggt
     3721 cttcatcgcg aaagcagcgc acgatctgga aatatttctc gaaaccgctc accatcagca
     3781 gttgcttgaa gagttgcggg gattgaggca gcgcgtagaa gctgccctgc tgg
//
LOCUS       DASTMK010000073        19933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_612223, whole genome shotgun sequence.
ACCESSION   DASTMK010000073 DASTMK010000000
VERSION     DASTMK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19933
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612223"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFQ79_02910"
     CDS             complement(<1..230)
                     /locus_tag="VFQ79_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU0138628.1"
                     /translation="MTDLRKKMLEELQRRNYSQTTVNAYLKIVESFANYFQRSPNQLG
                     PAEIRAYQVYLLKERKLGARTVGHHTAALRFF"
     gene            622..1854
                     /locus_tag="VFQ79_02915"
     CDS             622..1854
                     /locus_tag="VFQ79_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0138629.1"
                     /translation="MHRYSVWVPTATMLLASLISYIDRNTLALLAPTILRETGLTAQE
                     YGFVISGFSVLYMIGNPVWGKCLDGIGLRVGMTLAVSFWTIASAAHAFVGGFWSLAAA
                     RSALGFGEGATFPGALRTVVQTLPPSRRSRGTAIAYSGGSLGAVITPLVITPVAIWWG
                     WRFAFLFTGFVGVCWLLLWMFVSRRADVRTVPVHQQQEPLEPLRFRDRRVWGFMAAYA
                     LGAMPIGFILYSAAIYLGNVREVSQLTLGKLLWAPPLGWEAGYFFWGWLCDRCVAKGS
                     AGVPLFRTLLGIAAILSLSLAFVTAIPTTAGVMVQLFFSMFIAAAFVILPIAHATTAL
                     SARHAGLIAGLGAGSWSAVLALLMPVFGRLFDLERYQDAFGIAAVIPCLGFAGWWIST
                     RSKKEKGACGLTASATTQ"
     gene            complement(1871..2311)
                     /locus_tag="VFQ79_02920"
     CDS             complement(1871..2311)
                     /locus_tag="VFQ79_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU0138630.1"
                     /translation="MAITKWDPFGDVEGFPPMRLFQDAVSRLLSEPPASRPWAPPVDI
                     FETENELVVKADLPDVDQKDIDINVENGTLALKGERRFEKKTDNQGYHRIERSYGSFV
                     RYFGLPESVDTEKVRAEYKNGVLTVTLPKKEAAKPRAIKVEVAS"
     gene            complement(2395..5004)
                     /locus_tag="VFQ79_02925"
     CDS             complement(2395..5004)
                     /locus_tag="VFQ79_02925"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0138631.1"
                     /translation="MSPPAANCHYKRKALPVGILIVFGLAAARAEYRAPAGEKPAMRR
                     PGAASILPGGRVIVPVGRQYVTGAGPFGIAISPDGKRVATPNGGPNRYSVSVIEKSGD
                     RWRAKEYVASGDAQGDSGGDDWHSVFMGLAFEDNGTFWVSEGNSGRVRALNPSNGETR
                     EVIDLNRDGFQNSYSGGLAIDRERRRLYVVDQANDRVAVIDLSKKRILASVRVGRLPF
                     AIALAPDGNRVYVTNPGMLEYRPLPGVDPKRIRDTGLPFPAFGFPSPEAASGVRRETP
                     GGAIEVPPLGDPNSGQSNSVAVINVSGPGPPAVEKFVRTGLAFGEHSQGGSSPSGILA
                     TTDRVYVSNAHNDSVSVLNARTLELETEIRIRIPTLEYLRGVLPIGLAYHEASGWLLV
                     AEAGMNAVGIIDTRQNKLIGHLPVGWFPTAIAIREDDLYVTNIKGHGTGPNATEQRAF
                     RQSFQGSLRRGTLTIAPLPGTDELAKHTGLVYDANGFLPRKEAAKALPEAIRYVVLIV
                     KENRTFDEVLGDITTASNGSVAGAPLLARFGEHGIIYPDYGSKRPRLGLRNINVTPNH
                     HAIARRWSFSDNFYASSEVDADGHHWLVGAYPNAWTESSLRAVHGGQSRRSVHPEGQP
                     EAGILWHHLERNGISFRNFGEGLELAGVDQGPGLRPTGARYLTNVPLPDPLYRNTSRE
                     YPQYNMNIPDQYRATRFIEEIERDFVKAGKELPRFLFLHLPNDHMANPRPEDGYPFEA
                     SYVADNDYALGRILEYLSKTPWWKQMAVFITEDDAQGGVDHIDSHRTLLLVASPYARK
                     NYVSHVNTSFPGLLKTIFRLLGMPPLNLFDAVASDLADCFTDEVDFAAYEAVPIARKD
                     LFDPERAREPAER"
     gene            5003..5785
                     /locus_tag="VFQ79_02930"
     CDS             5003..5785
                     /locus_tag="VFQ79_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEU0138632.1"
                     /translation="MYRRAFMYKMGGLGLMPASTSFSQAPAGRGTRFYVLDNYYLRNG
                     SQVARMHDFLRGVAPVLKEIHAGPVIFLEALVAPHMPQIATIAGFQSLQEWEAARRKG
                     SAHEAFIKALRTLESGEEAPYEHFSETLLEATDYSPEIAAPESPPKTPRIFELRVYHS
                     PTFRQLRALHERFAGPEIRIFHRVGVHPILYTSGLTGQNLPNLTYLIPFDNLAAREKA
                     WQAFSADPEWVKVRRESIERSGQISSVMNISLYKAADYSPIR"
     gene            complement(5816..6174)
                     /locus_tag="VFQ79_02935"
                     /pseudo
     CDS             complement(5816..6174)
                     /locus_tag="VFQ79_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            complement(6274..7590)
                     /gene="rseP"
                     /locus_tag="VFQ79_02940"
     CDS             complement(6274..7590)
                     /gene="rseP"
                     /locus_tag="VFQ79_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU0138633.1"
                     /translation="MVIFQNIWWLLVLIGVMILIHELGHYWAARLFDVKIEAFSFGFG
                     PRLFGFRRGETDFRFSLILFGGYVKMAGEQPGEETNDPRAFTAKPRWQRLIIAFAGPA
                     MNVVLAVALVTGLYMVRYPKMPASERDAIVGHVVPDSPAAKAGIQEGDEIVRVDGIQA
                     PTWEDVFIREISNAGNPISLVLNRDGQQIRATVTPILDERTGVGFAGWSEQSEIQVSG
                     YVPGVDAARNAGLIRGDILLAINGQPIRSSHKLNEVLTASAGKPVEILYSREGVRHKT
                     AVQPEKSSATGEERWMIGVQLEPRVIMTRLSFPEALRESIDRNLKSATLIYKFLQGIV
                     ERRMSAKSLEGPIRIAQLSGEAAREGPEAFIGLMAMVSLNLAIFNLLPIPILDGGVIL
                     LLLIEMLMRRDLSLQVKETVFKLGFVFLMAVVMFVLYNDISKILPG"
     gene            complement(7606..8808)
                     /gene="dxr"
                     /locus_tag="VFQ79_02945"
     CDS             complement(7606..8808)
                     /gene="dxr"
                     /locus_tag="VFQ79_02945"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU0138634.1"
                     /translation="MQSVCLLGSTGSIGQSTLDVIRRSPERYRVCSLVAGRNIRLLTT
                     QIKEFRPRLAVMATAADLANLTTELEDAGIPKSEWPELAWGSRARVEAATDGEVDFVM
                     SAIVGVAGLEATYEAVRKGKTVGLANKEVLVASGSLVMAAAAEAGTELIPVDSEHNGA
                     HQCLRAGERREATRLILTASGGPFRDLPAEKFHTVTPAQALNHPTWKMGQRITIDSAT
                     MMNKGFEVIEACWLFGFSADEVDVVIHPQSSVHAMVEYADGSILAQASTTDMRMPIQY
                     ALTYPERSKSAVPRMDWSVARTWNFFPPDPARFPCLRLAFEAQKAGSAASCALNASDE
                     IAVQAFLEERISFDEIPQIVEETISAMPGRDPRSIQDVLEIDKEARSVARQTASRRAA
                     KRDTLARA"
     gene            complement(9053..9949)
                     /locus_tag="VFQ79_02950"
     CDS             complement(9053..9949)
                     /locus_tag="VFQ79_02950"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0138635.1"
                     /translation="MAKLGFLGLGIMGYPMAHNLLKAGNEVALWSHTAEKAQELAGKG
                     KGKACSTPKEVGEQADIIFLCVGDTAMSEECILGEQGIIHGAKPGTIIVDASTVSPSR
                     SRFIGERLAEKKILFLDAPCTGSKPGAENATLTFMIGGDKAIYEKVKPHLEEMGKQLY
                     YCGGPGMGLHAKLSQNLILSNILQAFNEGLVLSTKAGVDPELMLDILNNSAARSGLIA
                     FKAPYVFKRNFETNFSVKWMHKDIGLMLDSAKELNVPVPLTGLTQQLFQTAISKGFGE
                     DDICSTIKVLEEMAGVEVKKTE"
     gene            complement(10369..12990)
                     /locus_tag="VFQ79_02955"
     CDS             complement(10369..12990)
                     /locus_tag="VFQ79_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138636.1"
                     /translation="MRFGGGKRWLDDLDRDIQDHIELETRENIERGMAPDEARYAALR
                     KFGNTTRVLEDTRAVWHPVWLEQLTQDIRYALRTLRRQPAFTAVVVATLALGIGMNTA
                     VFSVLNTVLFRPLPYPDAGRLIWLANYNQQWKQDNWVARTDYRTWKQQALSFEKMAAY
                     GNQDLAVVSGGESSQERIASVTGDFWEIAGAQPASGRLFQAGEWNTAVLSRSLFERRF
                     GGDAGVIGKTITVNGWPFTIVGVLPKNFRFLFPQQFAGGDEVREIDAYIPIPDTLMAM
                     PPSGVQRWEEAIRRFGPAPFWIVVVGKRRADVPFEKARAEMQTIYSRIAEQDPNPLRV
                     FRILRFTALQDKLAGETRRALVVLLVAVGFVLLIACANIANLLLARASARQREIAIRA
                     AVGAGRMRVVRQFLAESVLLALLGCAAGLLLAQWAISVLPRLAPQAVPRLAETTIDGR
                     VLAFTLAVSVFSGLFCGLAPVIYAWRAEVFDVLKAETGTSSAASGRIRLRGVLVASQI
                     ALAVVLLAGAGLMLKSFWRMNAKPAGFAPDKILVMRVALSGSQYSTWSPKQAYTEELL
                     QTLQALPGVEAAGVDAGTLNTNVQVDDGVPSPGGGVPASIRGVSPGYLRAIGIPLLKG
                     AWPERGNLFGVVVNEAFARRIAGGEATGKHLSGSILNDTITGVVADFKTWQLDAEPLP
                     EVYLPYERLPLNRSMRVVVRAEDGAAALAPVVRQLVAGIDRTQPVYEFQTLQDALSAS
                     IAPRRFNLFLLGAFAATALLLAVIGIYGVMAYSVSQRTREIGIRMALGARRGEIIGMV
                     VRQGMKLALAGIVAGIPAGIALTRLMKSLLYGVNPGDPWIFALVVAALVAAALLAAGG
                     PALKAARVDPLTALRYE"
     gene            complement(12987..13346)
                     /locus_tag="VFQ79_02960"
     CDS             complement(12987..13346)
                     /locus_tag="VFQ79_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0138637.1"
                     /translation="MSTRDEDRIEILQGTLDLLILRTLSLGPAHGHAIAKTIERNSEE
                     VLQVEQGSLYPALHRLIQRGWISAEEGISENNRRAKYYRLTARGRKQLLVETTKWDKL
                     ARAIGRILRPAQQENGA"
     gene            complement(13396..15666)
                     /locus_tag="VFQ79_02965"
     CDS             complement(13396..15666)
                     /locus_tag="VFQ79_02965"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HEU0138638.1"
                     /translation="MTEPQTSTVYPPFRSLKPAVYDWQVVYLDEKGAALGVSRTRRFT
                     VPNSAPELLMPDVQALKARLAGVRPRLFLGDGRLDRLRAAVAKGSVPSWDRLRQAADA
                     ALEEDLYPEPPVLGSGAENWLRTFTPGKVGSAHLARTALAYKVTGEAKYRNAARRWLL
                     HLASWDPKGITSHNLKLPGGGRGNDEASMPMLERMSLAWDWIGDTLTPEERKKVLASM
                     TERGNQVLRVLEDEDFLSHPFGNHSGRSLAFLGPAGLAFLGDIPEAERWLDYVLRALL
                     TSYPSFGGDDGGWAQGLSYWSFYIYSHANFAMALRQATGTDLFARPFFRNTGYFGLFF
                     APPYAPRGGFGDGAYHRPNESGGVLVETLSNAHHDPALKWYARGIHEMGERNKTKWRE
                     WFVEDVYETLQAADAPALEPEPPSKLDGSKHMADIGWVAMHSALGDAKNDVWALFKSS
                     RFGSFSHSHADQNSFQLYAYGRALAIDSGYYPSYGTPHDNLWTRQTRAHNSVLVNGRG
                     QPPHTWEAAGNIDLYERHGIVTLVRGQAAGAYNLPQPPGIARLWQKHLREPIPPMDPE
                     VKNFERTLAFVASDTRPILVVHDSLNTNGPARFSWLLHALNRMETDSRSGTVFVRDGD
                     ARLVVRLVASVPFHFGQRSGFPIKPEFSANTAYVLGDEPFADQWHLQATTGQSSDEVK
                     FLAVFVPYRASEPEPKIELLSADQAIGFRVAGTEVAAWWGAGSRGRIAASGITGEGRL
                     AINAGEADKAVHVTVP"
     gene            complement(15965..16342)
                     /locus_tag="VFQ79_02970"
     CDS             complement(15965..16342)
                     /locus_tag="VFQ79_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138639.1"
                     /translation="MDYEALALVNGEVHLYLNTAGPIDAKELQARLTYAYSHQREHRT
                     SSDPWESAFFDPRFIPSEAEFLKERDRVVEIMLSAISGQWDASLIYYPPGQPSAPYHW
                     SRSEGILHPYRTVNPDISEESYE"
     gene            16426..17718
                     /locus_tag="VFQ79_02975"
     CDS             16426..17718
                     /locus_tag="VFQ79_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU0138640.1"
                     /translation="MSDRKDKHPALRPPTAVLTRGFDPSLSVGSARPAVFRSSTYVFS
                     SPEAAERAFAVMSGREQRAENETVDLIYSRFNHPNAEILEDQIVPLEKGAHAAAVFNS
                     GMAAIMTSILTVARPGQQIVYTVPLYGGTQHFIHEFLEPFGIKGTPVPAGDGDALDEA
                     IRSTGNLAVVFIETPANPTMTMTDIHRAVAAAAGRGDGNRALVMVDNTILGPTFQHPL
                     DLGADLSLYSATKYLSGFSDMIGGVAIAADGELIRKIRGRRGLFGNILQPDECWMLDG
                     RLPTVALRMNRASKNAQRIAEQLAGHKEVRKVYYPTLFDDAEQIRIRAAQCDFPGALF
                     SVDLYGGKPAAFDFLRNLKIARNAVSLGGVETLACHPKTTTHSSVSQQELELAGVTDG
                     LVRISVGIEDWRDLLADFEQALDAIPAEAGVGQLRSGS"
     gene            17784..18389
                     /locus_tag="VFQ79_02980"
     CDS             17784..18389
                     /locus_tag="VFQ79_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEU0138641.1"
                     /translation="MPIAEPLAKVAALAKTAGVLRPRDLDAHGIARQYLRLAEQQGLV
                     VRSGRGLYTAADAPVTELHTIAETSKRVPRGVICLLSALRFHDIGTQNPFEVWIAIGE
                     KDRRPAAEFPPLRVVRFSKQSLAFGQEIHDIEGVPARIFSVAKTVADCFKYRNKIGLD
                     VALEALRECLKQRKATTADLWAAANVCRVANVMKPYMEALV"
     gene            18386..19369
                     /locus_tag="VFQ79_02985"
     CDS             18386..19369
                     /locus_tag="VFQ79_02985"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEU0138642.1"
                     /translation="MKPVKPRNLAASIRQKLLNTATKQREDFGLVLTRYGLERLLYRL
                     SQSRYCDQFVLKGAMLFQIWTNTLHRPTRDLDLLGYGNPSPERCLAVFRELCGLVVPD
                     DGLDFPAEAISVEKIKEDQEYEGIRVRLLARMANIRIPLQVDVGFGDALPARPGVLNY
                     PTLLPMPAAQIQVYPMEAVIAEKLEAMVHLGMLNSRMKDFFDIWFLARTFSFESSALA
                     DAIRITFERRGTQLDPRGFDALTAELSVDTSKLIQWQAFLNKGNLVAPESFAGVIGAI
                     LGFVSFPLHASIAETRRPASWPPGGPWLEANRKNLGSESPIGVPDQEVGKL"
     gene            19366..19926
                     /locus_tag="VFQ79_02990"
     CDS             19366..19926
                     /locus_tag="VFQ79_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU0138643.1"
                     /translation="MKPIETLDVRSRPEWRKWLAKNHSSRSEIWLVFHKSHTGTSTLS
                     YDDSVEEALCFGWIDSIIKRLDDSRFARKFTPRKTDSKWSSINRKRYADLQSRGLLAA
                     PGLNRAPTSRSGDAPPRVPWAIPSYMEEALEANPRAAQYFEQLAPSYRRTYIGWIDSA
                     KRQETKEKRLREALTLLAAGKKLGLK"
BASE COUNT         4060 a         6063 c         5722 g         4088 t
ORIGIN      
        1 aaaaagaaac gcagtgcggc cgtgtggtgt ccgacggttc gcgccccaag ttttctttcc
       61 ttgagcaggt agacctgata ggcgcggatc tccgctggtc cgagctgatt cggagaacgt
      121 tggaaatagt tcgcaaacga ctccacgatc ttcaggtagg cgtttacggt agtctgggag
      181 taatttcgac gctggagctc ttccagcatc ttttttcgaa gatcggtcac agggacctcc
      241 ttgtgaccca cacactacgt caaacactga tggaaatgcc gaacctactg acacgcgtag
      301 cgtccgccgc gcgggcggct tcgttctgac ggcgaagagt ttactgatcc tacaagggac
      361 attaagtcgg ggtgtcggaa gtggccgggt tggacgctgt tgtcgagtcg atgaaggcat
      421 gcactcacgg caaggtgatg ctttgaaact tggacttccg gcaacattat ggtttatccg
      481 cgtcgggaga tgcccggacg gcgaaatgcc aacaaaggag ttgctgaggc atctgcggcg
      541 aggttatggt ttattcgcgg caaagatata gcttaagtca cagcagatga aaatggcgaa
      601 gggtgatatt ctgtttgagg cttgcaccgc tactctgtct gggttccaac agcgaccatg
      661 ctgttggcgt cgctcatcag ctacatcgat cgcaatacgc tcgcgctgct ggcgcctacg
      721 attctccggg aaacagggct caccgcccag gaatacggat tcgtcatctc cggattttcc
      781 gtgctctaca tgattggaaa tccggtgtgg ggaaagtgcc tcgacgggat cggtctgcga
      841 gtgggcatga cactggcggt ttcgttttgg acgattgcct ccgcggcgca cgcctttgtg
      901 ggaggttttt ggagccttgc cgcggcaagg tcggcgctgg gttttggaga gggagcgacg
      961 tttccaggcg cactgcgcac cgttgtacag acgctaccgc cgtcgcggag gtcccgcggc
     1021 acggccattg cctatagcgg tggctcactg ggcgccgtca ttacgccatt ggtgatcacg
     1081 cctgtcgcca tctggtgggg atggcggttc gcgttcctgt tcaccggatt cgtgggtgtc
     1141 tgctggctgc tgctgtggat gttcgtgagc cgccgggctg acgtgcgaac ggtgccggtg
     1201 caccagcagc aggagccgct cgagccgttg cgtttccgcg accggcgcgt atggggcttc
     1261 atggccgcct atgccctggg cgccatgccg atcggcttca ttttatacag cgcagcgata
     1321 tacctgggca acgtaagaga ggtgtcgcaa ctgacccttg gtaagctgct ctgggcgccg
     1381 ccgctcggat gggaagccgg ctatttcttc tggggatggt tgtgcgaccg ctgcgtcgcc
     1441 aagggaagtg ctggagtccc attgtttcga acgctgctgg gaattgccgc gatcctgagt
     1501 ctctctcttg ccttcgtcac cgccattccg acgacagcgg gtgtgatggt gcaactgttc
     1561 ttctcgatgt ttatcgccgc agctttcgtg atcctgccga ttgcccatgc cacgacggcg
     1621 ttgtccgccc ggcatgcggg cctgatcgcc ggcctcggcg cgggctcgtg gtccgccgtc
     1681 ctggcgcttc tgatgcctgt attcggaagg ctattcgatc tggaacgcta ccaggacgcg
     1741 ttcggcatag cggcggtgat tccgtgcctg ggttttgccg gatggtggat ttccacgcgg
     1801 agcaaaaaag aaaaaggcgc ctgcgggctc accgcaagcg ccacaacgca atagtggttt
     1861 ccgctcccgc tcagcttgcg acttccactt taatcgcacg cggtttggcg gcctctttct
     1921 tggggagcgt caccgtcagc acgccatttt tgtactcagc ccgtactttc tcggtgtcca
     1981 cggattccgg cagcccaaaa tagcggacga acgacccgta gcttcgctcg atgcggtggt
     2041 agccctggtt gtcggtcttc ttttcgaacc ggcgctctcc ttttagagcg agcgtgccgt
     2101 tttccacatt aatgtcgatg tccttctgat cgacatccgg caggtccgcc ttcaccacga
     2161 gttcattttc cgtttcaaag atgtcgaccg gaggcgccca gggccggctg gcaggcggct
     2221 cgctcaacag ccgcgagaca gcatcttgaa agagcctcat ggggggaaag ccctccacgt
     2281 cgccaaacgg atcccatttc gtgatagcca tattcaggtt tcctccttgc aaatctttat
     2341 cacaattaca atataaaacc tcagtgtatc attgtcaagt aattctaagc acaattatcg
     2401 ctccgcaggc tccctcgctc tctccggatc aaaaaggtct ttcctggcaa tcggaaccgc
     2461 ttcgtaagct gcgaaatcca cctcgtccgt aaagcaatcc gccaggtcgg atgccacggc
     2521 atcaaacaga tttagcggcg gcatgccaag cagccggaaa atggtcttca ggagtccggg
     2581 gaaactggtg ttgacatgtg agacgtagtt cttccgggcg tacgggctcg caacaaggag
     2641 caatgtgcga tgcgaatcga tgtggtccac gcccccctgc gcgtcgtcct ccgtgatgaa
     2701 cacggccatc tgcttccacc acggcgtctt ggacaggtac tccagaatcc ggcccagcgc
     2761 gtaatcgtta tcggcaacgt aagacgcctc gaaagggtat ccgtcttccg gccgcgggtt
     2821 ggccatgtgg tcgttcggca gatggaggaa caggaaccgc ggcagctctt tcccggcctt
     2881 cacgaagtct ctttcgatct cctcgatgaa acgcgtggcg cgatactggt ccggaatgtt
     2941 catgttgtac tgcggatact cgcgcgacgt attccggtag agcgggtccg gcaggggcac
     3001 gttcgtcaga taacgtgcgc ccgttggtct caggcccggc ccctggtcga cgccggccag
     3061 ctcgagccct tcgccgaaat tgcgaaacga aataccgtta cgctccaggt gatgccagag
     3121 gatcccggct tccggctgcc cttcgggatg caccgagcgg cggctctgcc cgccatggac
     3181 agccctcaac gaactttcgg tccaggcatt ggggtaagcg cccaccagcc agtgatggcc
     3241 gtcggcatct acctccgagc tggcatagaa gttgtcgctg aaactccagc gccgggcgat
     3301 cgcgtggtgg ttgggagtca cattgatgtt gcgaagcccc aggcggggac gtttcgaacc
     3361 gtagtccggg tagatgatgc catgctcgcc gaaacgcgcc agcaggggag cgcccgctac
     3421 ggacccgttc gaagcggtgg tgatatcgcc cagcacctca tcgaatgtcc ggttctcttt
     3481 gacgatcaga acgacgtacc ggatcgcctc cggcagcgct ttggcggcct ctttgcgagg
     3541 caggaacccg tttgcgtcat atacgaggcc ggtgtgcttg gccagctcat cggttcccgg
     3601 cagcggagcg atggttagcg taccgcggcg gagggagccc tggaaactct ggcggaacgc
     3661 gcgctgttcg gtcgcattgg gcccggtgcc atggccctta atgttcgtca catagaggtc
     3721 atcctcgcgg attgcgatgg ccgtcgggaa ccaacccacc ggcaggtgtc caatcaattt
     3781 attttgccgg gtgtcgatga ttcccaccgc gttcatgccc gcctcggcca ccagcagcca
     3841 cccggaagct tcgtgatacg ccaaaccgat cggcagcacg ccgcgaagat attcgagggt
     3901 gggtattcga atgcggatct ccgtttcgag ttcgagcgtg cgggcgttca ggacactcac
     3961 agaatcgttg tgagcgtttg aaacataaac gcggtccgtt gttgcaagga tgcccgacgg
     4021 gctgctgcct ccctggctgt gctcgccgaa tgcgagaccc gttcgaacaa acttctccac
     4081 cgccggcgga cctggcccgg aaacgttgat gacggccacc gagttcgatt gcccggagtt
     4141 gggatcgccc aacggaggga cctcaatcgc gccgcccggc gtttcccgcc gtactccgga
     4201 tgcggcttcc ggcgacggaa acccgaaggc cgggaaaggg agccctgtgt cccggatccg
     4261 ctttggatcg acgcccggca gcggccggta ttccaacatg ccgggattgg tgacgtacac
     4321 acggtttccg tccggcgcca gcgcgatggc aaagggcagc ctgcccacgc gcacggaagc
     4381 gaggatgcgc ttcttcgaca gatcgatgac cgcgacacga tcgttcgcct gatccacgac
     4441 gtagagacga cggcgttcac ggtcgatcgc gaggccgccc gagtagctgt tctgaaagcc
     4501 gtcccggttc aggtcgatga cctcgcgcgt ttcaccgttg gatgggttca gtgcccggac
     4561 gcggccggag ttgccctccg atacccaaaa cgtaccgttg tcctcgaagg cgagccccat
     4621 gaaaacgctg tgccagtcgt cacctccaga atcgccctgt gcatcgcccg aggccacata
     4681 ctccttggcg cgccaccggt ctcccgattt ttcgatcacg ctcaccgagt atcgattcgg
     4741 ccccccgttc ggggtggcga cacgcttgcc gtccgggctg atcgcgatgc cgaaaggccc
     4801 cgcgccggtc acatattgcc tgcccacagg aacaatcacc cgtcccccgg gcaggatgct
     4861 ggccgcgccg ggacgccgca tggccggctt ctctccggcc ggcgccctgt attccgcgcg
     4921 cgccgccgcc agcccaaaaa cgatcaggat tccaaccggc agggctttcc gtttatagtg
     4981 gcaattggcc gcgggaggtg acatgtaccg aagagcgttc atgtacaaaa tgggaggact
     5041 cggactaatg cccgcttcaa cttcattttc gcaagccccg gcgggccggg gaacgcgctt
     5101 ctacgtgctc gacaactact atcttcgcaa cggatcgcag gtcgcgcgga tgcacgactt
     5161 tttgcgcggc gtggcgccgg tgctcaaaga gattcatgcg gggccggtga tttttctgga
     5221 ggcgctggtg gcgccgcaca tgccgcagat cgccacgatt gccggatttc agtcgctcca
     5281 ggaatgggaa gcagcgcgca ggaagggatc cgcgcacgaa gcgttcatca aggcgctccg
     5341 gactctcgaa tccggtgaag aagcacccta cgagcatttt tcggagacgc tgctcgaggc
     5401 cacggactac tcaccggaga tcgccgcacc agagtccccg ccgaagacac cgcgtatctt
     5461 cgaactgcgc gtatatcatt cacccacctt ccggcagttg cgcgcgctgc acgagcgctt
     5521 cgccggacct gagatccgga tcttccaccg ggtgggagtg catccgattc tctacacttc
     5581 cgggctcacc ggacagaacc tgccgaatct tacctatctg atcccgttcg acaatctggc
     5641 ggcccgggag aaagcatggc aggcattttc ggcagatccg gaatgggtga aagtgagacg
     5701 cgaatccatc gagcgcagcg gccagatctc cagcgtgatg aatatctcgc tctacaaagc
     5761 agccgactac tctccgatcc ggtagggctc gcctgcatct cagatacaga cgctgctagt
     5821 ccaggaggac tcgcttccat tccaccttgg gtttttggaa attgacgagc agggccacgt
     5881 gaagaccgga ggccttcagg taattgatgc attgcgccag atgctcgttg gccaagtgat
     5941 cgacgcactt caattccaca atgagctgtt cctcaaccac cagatcggcc acatactcgc
     6001 ccatgtactg ccctttgtag ctgaccggaa atgagacttg agccttggcc ctcacaccgc
     6061 gcgttcgtag accttctcca ggaatccggc gccaagcaca ttagcaacct cgaaggccga
     6121 cccaatcacg gcttccgtca gttccgattc cgatcggtgt gcatccgtgt ccatccgtgg
     6181 ccctttccaa tgggttgcag caacggggca aaaatctcaa caacaggatg gaatattagc
     6241 cacggatgga cgcggataca cacggattat agttcacccc ggaaggatct ttgaaatatc
     6301 gttgtagagc acgaacatca cgacagccat caggaaaacg aagccgagct tgaagacggt
     6361 ctctttcacc tgaaggctca ggtcccgccg catcagcatt tcgatcagca acaggaggat
     6421 gacgcctcca tccagaatcg gaatgggcag caggttgaaa atggccaggt tgaggcttac
     6481 catggccatc agtccgatga atgcctccgg tccttcgcgc gcggcttctc ccgaaagctg
     6541 cgcaatgcga atcgggcctt cgagcgactt cgccgacatc cgccgctcca caatgccttg
     6601 caggaacttg tagatgagag tggcgctttt cagattccgg tcgatcgatt cccgcaacgc
     6661 ctccggaaaa ctcagccggg tcatgatgac tcgcggctcg agctgaacgc cgatcatcca
     6721 gcgttcctcg ccggtggcgg aggacttctc cggctgcacc gcggtcttgt ggcggactcc
     6781 ctccctggaa tagagaatct cgaccggctt tccggcgctg gcggtaagca cttcgttgag
     6841 cttatgcgag gaccggatcg gctgcccgtt gatcgccagc agaatatcgc cgcgtatcaa
     6901 tcccgcgttt cgagccgcat ccacgcccgg cacataaccg gacacctgaa tttcactctg
     6961 ttccgaccaa cccgcgaaac ctacccccgt tcgttcgtcc aggatcggcg taacggtggc
     7021 tcgaatctgc tggccgtccc ggttcagaac gagcgatatc gggttgcccg cattggagat
     7081 ctcccgaatg aaaacgtctt cccaggttgg agcctggatt ccgtcgaccc gaacgatctc
     7141 gtcgccttcc tggattcccg ctttcgccgc gggagaatcc ggaacaacat gcccgacgat
     7201 cgcatcccgt tcggaggcgg gcatcttcgg gtagcgaacc atatagagac cggtaacgag
     7261 cgccacggcc aggaccacgt tcatcgcggg cccggcaaac gcgataatca gccgctgcca
     7321 tcgcggcttg gcagtgaacg cacgggggtc gttcgtctct tcacccggct gctcgccggc
     7381 catcttgacg taacctccga acaaaatgag agagaagcga aaatcggtct ctcccctccg
     7441 gaagccgaag agccgggggc caaacccaaa gctgaatgcc tcgatcttta cgtcgaagag
     7501 ccgggcggcc caataatgtc ccaactcgtg gatgaggatc atcacaccga tgagaaccag
     7561 cagccaccag atgttttgaa aaataaccat gatgcctttc ttttttcagg cccttgccag
     7621 cgtatcgcgt ttcgctgcgc ggcggctggc cgtttggcgc gctaccgacc tggcttcctt
     7681 atcgatttcg agcacgtctt gaatggagcg gggatcgcgg ccgggcatcg ccgaaatcgt
     7741 ctcttccacg atttgtggga tttcgtcgaa ggaaatccgc tcctcgagaa aggcctgcac
     7801 cgcgatttca tcggatgcat tcagagcgca ggatgccgcc gaaccggctt tctgcgcctc
     7861 aaacgcgagc cgcaaacacg ggaaccgggc cggatccggc ggaaaaaagt tccaggtccg
     7921 cgctacgctc caatccatcc gcggaactgc ggacttgctt ctctccggat aggtgagcgc
     7981 atactgaatg ggcattcgca tgtccgtggt cgaggcctgc gccaggatgc tgccgtcggc
     8041 atattccacc atcgcgtgca ccgaagattg cgggtgaatc accacatcca cctcatcggc
     8101 ggaaaatccg aacagccagc aggcttcaat cacctcaaag cctttgttca tcatggtagc
     8161 cgaatcgatg gtgatccgtt gccccatttt ccatgtaggg tgattcaagg cctgcgcggg
     8221 agtaacggtg tggaattttt ccgcgggtag atcccggaaa ggtcctcccg atgccgtcag
     8281 aatgagccgc gtagcttcgc ggcgttcgcc ggcacgcagg cattgatgag ccccgttgtg
     8341 ctcgctatct acggggatca actctgtgcc tgcttccgcg gccgcggcca ttaccaggga
     8401 accgctcgcc accaggactt ccttgttcgc caatccgacg gttttccctt tccgcacggc
     8461 ctcatacgtc gcttccagcc cggccacgcc tacgatggcg gacatcacaa aatccacttc
     8521 cccgtcggta gcggcttcga ctcgtgcccg ggaaccccag gcaagctccg gccattcgga
     8581 tttcggaatc cccgcatctt caagctcagt agttagattc gccagatccg cggcggttgc
     8641 cataactgcg aggcggggac ggaattcctt gatttgagtg gtgagaaggc ggatatttct
     8701 gcccgcgacg agcgaacaaa cccggtaacg ttcagggctc cggcgaatta catccagcgt
     8761 ggattgccca atagaacccg tggagccaag caggcagact gattgcatct agacctcaac
     8821 attctatcac tcgccgggta aacccactcc taccgaaaat catattgctc gcggctaact
     8881 ggatgcttgg ggttcggatt tcatccgaag agaagaaata gccctttcgg gcgccgggga
     8941 ggttgcttca ggggtcttta tgaggttagt ctcgctttgg tgggagttgc ctccccggct
     9001 tacttccaat aatagaatat ggtttttcag aatgcaacag aaaattgtag atttattctg
     9061 tcttcttgac ctcgacccct gccatttctt ctaacacttt gattgtagag caaatatcat
     9121 cctctccaaa tcccttggaa attgccgttt ggaaaagctg ttgggtcagc cctgtcagag
     9181 gaacgggcac attcagctct ttggcggaat ccagcatgag cccgatgtcc ttgtgcatcc
     9241 acttcacgga aaaattggtt tcaaagttcc gcttaaacac gtaaggggct ttaaaagcaa
     9301 taagtcccga gcgcgccgcg ctgttattga ggatgtccag catcaactcc gggtccactc
     9361 ctgccttggt cgagagcacc aggccctcat tgaatgcctg cagaatattg gagaggatca
     9421 gattctggga gagtttcgca tgcaggccca ttcccggccc accgcagtaa tacaactgct
     9481 tacccatctc ttccagatga ggcttcactt tttcatagat tgccttgtcc cccccgatca
     9541 taaaggtgag cgtggcgttt tcggcgccgg gctttgagcc ggtgcagggc gcatccagaa
     9601 acaggatttt cttctccgcc aggcgttccc caatgaaccg gctccggctg ggagacacag
     9661 tactggcgtc cacaatgatc gttcccggtt tggcgccgtg aataatgccc tgttctccga
     9721 ggatgcattc ctcggacatc gcggtgtcgc caacgcaaag gaagatgatg tccgcctgct
     9781 cgcctacctc tttgggcgtc gagcaggctt tgcccttgcc tttccctgcc agttcctgtg
     9841 ctttctccgc cgtgtgagac cacagcgcga cttcgttgcc cgccttcaac aggttgtggg
     9901 ccatcggata tcccatgatt cccagaccca gaaaacccag cttcgccatt cctatcctcc
     9961 caaaacgctc gtgatgtgta agttctccat tctacgatgg cgtctcccgg tgggacaatt
    10021 tcgagcggag tggctaatcg gagcggtggg aagtcgtgtc gcgtcctgac aggtgcctca
    10081 gcaacacaac atcggaaagc tgaaattcgg cggtgccgag gttttggaga tcttcagagc
    10141 cggctccgat gtgttcacta accatggacc gcaactcatc aatagtgatt ccgagcaaac
    10201 gggcggcttc agcggcggag tagtaacctt ttgggggttt acggagattc aagtttggcc
    10261 tcgttctccc agattctatg ctttccgcgc aacgatccca attggaaaca tgccttagtt
    10321 tcccggcgca atgtgctgat gagaggccgg cgacagccaa agcggcggct attcgtaccg
    10381 cagcgcggtg agtggatcca cccgtgcggc cttcagtgcc gggcctccgg cagccaacaa
    10441 ggcggcggcg accagggcgg cgacaaccag ggcgaaaatc cacggatcgc cgggattcac
    10501 gccgtaaagc agactcttca tcaggcgtgt gagcgcgatg ccggccggaa tgccggcaac
    10561 gatgccagcg agcgccaact tcattccctg cctcacaacc ataccgatga tttctccgcg
    10621 gcgggcgccg agagccatcc ggatgccgat ctcccgcgtg cgctgcgaaa cagagtaagc
    10681 catcacaccg taaatgccga tcaccgccag cagcaatgcc gtcgccgcaa aggcgcccag
    10741 caaaaacagg ttgaaacggc gcggcgcaat cgaagccgac aatgcgtcct ggagcgtttg
    10801 gaactcgtag acaggttgag tgcggtcgat cccggcaacc agttgccgga caaccggcgc
    10861 caacgcagcg gcaccgtcct cggcacgaac gacgactctc atcgagcggt tcaagggcag
    10921 tctttcatag ggcaggtaga cttcgggcag cggctcggcg tccagttgcc aggtcttaaa
    10981 atcggccact acgcccgtga tcgtatcgtt cagaatcgag ccgctgagat gtttgccggt
    11041 cgcctctcca ccggctatgc ggcgcgcaaa ggcctcgttg acaaccaccc cgaacaggtt
    11101 gccgcgttcg ggccaggcgc ctttgaggag agggattcca atggctcgaa ggtagccggg
    11161 cgacactcct cgaatcgagg cgggtacacc gccgcccggt gaaggcactc cgtcatccac
    11221 ctgcacgttt gtattcagag taccggcatc gactccggcc gcctccacgc ccggcagagc
    11281 ctgaagcgtt tgcagcaatt cttccgtata ggcctgcttc ggcgaccagg tgctgtactg
    11341 cgagcccgat agtgcgactc gcatgaccag gattttgtcc ggcgcaaaac cggctggctt
    11401 tgcgttcatg cgccaaaagc ttttgagcat cagcccggca ccggccagca gaacgacggc
    11461 cagggcgatc tgcgaagcca ctagcacacc acgaaggcga atgcgaccgg aggccgcgga
    11521 agacgtgcct gtttcagcct tgagcacatc aaaaacttcg gctctccaag cataaatcac
    11581 aggagccaac ccgcagaaaa ggccggagaa aacggaaacc gccagcgtga acgcgagcac
    11641 ccgcccgtcg atagtggttt ctgcgaggcg cggcaccgcc tggggcgcca gccggggcag
    11701 aacggagatg gcccactgcg ccaaaagcag accggcggca cacccgagca acgccagaag
    11761 cacgctctcc gcaagaaatt gccggaccac ccgcatccgt ccggcgccaa ccgccgcgcg
    11821 gatggcgatc tccctttgcc gcgccgatgc acgcgctaac agaagattgg cgatattggc
    11881 gcaggcgatc agcaaaacaa atccgacggc aaccagaaga actacaagcg cccggcgcgt
    11941 ttcacctgcc agtttgtcct ggagcgccgt aaagcgcagg atgcggaata cgcgcagcgg
    12001 attcgggtcc tgctccgcaa tgcgcgaata gatcgtttgc atttccgctc gcgctttctc
    12061 gaacggaaca tccgctctcc gtttcccgac gacaacaatc cagaacggcg ccgggccaaa
    12121 acgtctgatg gcctcctccc agcgctgcac tccggaaggc ggcatggcca taagcgtatc
    12181 cggaatcgga atgtaggcgt cgatttcccg tacttcatct ccgcctgcga actgctgcgg
    12241 gaacagaaac cggaaatttt tcggcaacac ccccacgatg gtgaagggcc atccgttcac
    12301 ggtaatcgtc tttccgatca ctccagcatc gccgccaaac cggcgctcga aaagactccg
    12361 cgacaacacg gccgtattcc actcgcctgc ctgaaacagg cggccggaag cgggctgagc
    12421 gcccgcgatt tcccaaaaat caccggtgac cgaagcgatt ctttcctggc tgctctctcc
    12481 cccggagacg accgccagat cctggttgcc gtatgcggcc atcttctcga atgagagcgc
    12541 ctgctgtttc caggtccggt aatcggtcct cgccacccag ttgtcttgct tccactgctg
    12601 gttgtagttc gccagccaga tcagccggcc ggcatccgga tacggcaatg gacgaaagag
    12661 cacggtgttc agcacgctga acacggccgt gttcatcccg atgcccagtg ccagcgtcgc
    12721 caccaccacg gcggtaaagg cgggttgccg ccgcaacgtc cgcaatgcat agcgaatatc
    12781 ctgcgtcaac tgttcgagcc agaccgggtg ccagaccgca cgcgtgtcct ccaaaacccg
    12841 tgtggtattt ccgaatttac gaagcgccgc atagcgcgcc tcgtcaggag ccattccgcg
    12901 ctcgatgttc tccctggttt cgagttcaat gtgatcttga atgtcccggt cgagatcgtc
    12961 gagccagcgc ttgccccctc caaacctcat gcgccgttct cctgctgcgc cggccgcagg
    13021 atgcggccga tggcgcgcgc cagcttatcc catttcgtgg tctccaccag caactgtttg
    13081 cgccccctgg cggtgagccg gtaatacttg gcccgccgat tgttttcgga gatgccttct
    13141 tccgccgaaa tccatcctct ctggataagc cggtggagcg ccgggtagag agaaccttgt
    13201 tcgacctgca atacctcctc ggagttgcgc tcgatggttt tggcaatcgc gtggccgtgc
    13261 gcggggccaa gggagagcgt ccgcagaatc agcagatcca gcgtcccttg caggatctcg
    13321 atgcggtcct catctctagt agacattcta ggccgaggtt acgcctcttc cagtagaatg
    13381 tcaaggtgaa ggtggctagg ggacagtaac gtggaccgcc ttgtccgcct cgcccgcatt
    13441 gattgccagc cggccttcgc cggttatccc cgaagccgcg atccttcccc ggctgcccgc
    13501 gccccaccat gcggccacct ccgttccggc tacgcgaaaa ccgattgcct ggtccgcact
    13561 cagcagctca atttttggtt ctggctcaga cgcacggtat ggaacgaaca ctgccaggaa
    13621 cttgacctca tcactcgatt ggccggtggt ggcctgaaga tgccactgat cggcgaacgg
    13681 ctcatcgccg agaacgtaag cggtgttcgc ggagaattcc ggcttgatcg ggaaaccgct
    13741 gcgctgcccg aaatggaacg ggacggaagc aacaagtcgc accaccagcc tggcatctcc
    13801 gtcgcggacg aacacagtac cgctccggct gtcagtctcc atgcggttta acgcgtgaag
    13861 cagccaggaa aacctcgccg gcccattggt gttcagggaa tcgtgaacca ccaggatcgg
    13921 cctggtatcc gacgcaacaa acgccagagt gcgctcaaaa ttcttcacct cgggatccat
    13981 gggcgggatc ggttctcgaa gatgcttctg ccagaggcga gcaatgcccg gtggttgcgg
    14041 caagttgtaa gccccggcag cctgaccgcg gaccagcgtc acgatcccat ggcgctcgta
    14101 aagatcgatg ttgcctgccg cttcccatgt atgcggaggc tgtccccggc cattgacgag
    14161 aacactgttg tgggcccggg tctgccgcgt ccagaggtta tcgtgtggcg ttccatagga
    14221 aggatagtat ccggagtcaa tcgccaacgc gcggccgtaa gcgtataact gaaacgagtt
    14281 ctggtcggca tggctgtggc tgaaggaccc gaaccggctc gacttaaata gtgcccaaac
    14341 gtcgttctta gcgtcaccca gcgcggaatg catggccacc cacccgatat cggccatgtg
    14401 tttcgagccg tcgagcttcg agggaggctc cggttcaagc gccggcgcgt ccgccgcttg
    14461 caatgtttcg tatacgtcct cgacaaacca ctcccgccac ttcgttttgt tcctctcccc
    14521 catctcatgg atgcctctgg cataccactt cagtgccgga tcgtggtgcg cattcgatag
    14581 cgtttctacc agaacaccgc ccgattcgtt ggggcgatga tacgcgccat ccccgaatcc
    14641 acctctcggt gcgtacggag gagcaaagaa aagtccaaaa tatcccgtgt tccggaagaa
    14701 tggtctggca aagaggtcgg ttcccgtagc ctgccgtaag gccatggcga agttcgcatg
    14761 gctgtaaatg tagaaagacc aatagctcag accttgtgcc cagccgccgt catcgccgcc
    14821 aaaactcggg taggacgtga gtaaagcccg gagcacgtag tcgagccacc gctccgcctc
    14881 cggaatatcc cccagaaacg cgagacccgc gggtccgaga aacgcaagag agcggccgga
    14941 gtgattgccg aagggatgcg acaggaaatc ctcatcctcc agaactcgca gcacctgatt
    15001 acctcgctct gtcatggaag cgagcacctt cttccgctcc tcgggtgtta atgtatcgcc
    15061 gatccaatcc caagccagcg acattcgctc cagcatgggc atcgacgctt catcgtttcc
    15121 acggccgcct cccgggagtt tcaagttgtg actggtgatg ccctttggat cccatgaagc
    15181 cagatgcagc aaccaacggc gagccgcatt ccgatacttg gcctcaccag tgactttgta
    15241 ggccagggcg gtcctcgcca ggtgcgcact gccaaccttg cccggcgtga aagttcgcag
    15301 ccagttttcg gcgccggagc cgagaaccgg aggctcggga tagagatcct cttcgagcgc
    15361 ggcatcggct gcctggcgaa ggcgatccca ggacgggacg ctcccttttg cgaccgccgc
    15421 ccgcagtcga tcgagcctgc cgtcgccgag gaacaggcgc gggcggacac cggcgagccg
    15481 ggccttcaaa gcctgcacat caggcatcaa cagctcaggg gcggaattcg gcaccgtgaa
    15541 gcgccgcgtt ctcgaaaccc ccagcgctgc gcccttctcg tcgaggtata cgacctgcca
    15601 atcgtagaca gcgggtttca aactcctgaa cggcgggtag accgtggagg tctgcggctc
    15661 ggtcaagaag ccattctttg atcctccgct ctcgcgaacc tcgaggcgat agctcgtagc
    15721 tttctcattt ggtgtccagc agaatcccgg cggattgatg cccactgtct gcgcctcggc
    15781 aggatatgcc gccaactctc cggaccgcgg cgctctttcg acgacttcaa ccgagtcagt
    15841 ccaacggaag cttgtcgagc tgcaaccgaa cgaaataagg ccaaccaccg cagagcaaaa
    15901 acaacgccga tatatattgg tcactccgac tctccttatc aattgtacgg acacaagaaa
    15961 ccggctattc gtagctttct tcagagatgt cagggttcac ggtccggtaa gggtgcagaa
    16021 tgccttccga cctggaccag tgataaggag ctgatggctg cccaggaggg taatagatca
    16081 ggcttgcgtc ccattgaccg gaaatagccg acagcataat ctccaccacg cgatctcgct
    16141 cttttagaaa ttcggcttcc gaagggatga atcgagggtc gaagaacgca gattcccatg
    16201 ggtcggagga agtacggtgc tccctttgat gactgtatgc gtaagtcagc cgggcctgta
    16261 gctctttcgc gtcgattggg cctgccgtat tgagatagag atggacttcg ccgttcacca
    16321 gggccagagc ttcgtaatcc aatggagctc ccacttatca agacggtata gcactaacga
    16381 ggcttcttct caaacccgcg atagacttga gttggagaag caattatgtc cgatcgaaaa
    16441 gacaagcatc cggcattgcg gccgcccacg gccgtgctga cgcgaggctt cgatccgagc
    16501 ctgtccgttg gatccgcccg cccggccgtc tttcgaagct ccacgtacgt tttttccagt
    16561 cccgaagcgg ccgagcgcgc tttcgcggtg atgagcggca gagagcagcg ggccgagaac
    16621 gagacggtcg atctcatcta ttcccgcttc aatcatccga acgcggagat cctggaagac
    16681 cagatcgttc ccctcgagaa gggcgctcac gcagccgccg ttttcaactc cggcatggca
    16741 gccatcatga cgtcgatcct gacggttgcc cgccccggac agcagattgt ctacaccgtg
    16801 cctctgtacg gaggcacaca gcacttcata cacgagtttc tggagccgtt cggaatcaag
    16861 ggtacgccgg ttccggcagg cgacggtgac gcgctggacg aagcgattcg ctcaaccggc
    16921 aatctggcgg ttgtttttat cgaaacgccc gccaacccga cgatgacgat gaccgatatc
    16981 catcgagcgg tcgcagccgc cgccggtcgc ggagacggca accgggctct ggtcatggtc
    17041 gataacacca tcctcggccc cacgtttcag cacccgctgg atctgggtgc ggatctttcg
    17101 ctgtattcgg ctacgaagta cctgtccgga ttcagcgaca tgatcggcgg cgtcgccatc
    17161 gctgcggacg gagaactgat acgcaaaatt cgcggccgcc gcggcctctt cgggaacatc
    17221 cttcagccgg atgaatgctg gatgctggat ggacggcttc ccaccgttgc cctccgcatg
    17281 aatcgcgcca gtaaaaacgc gcaaaggatc gcggaacaac tggccgggca caaggaggtc
    17341 cggaaggtct actaccccac gctgttcgac gatgcggaac agatcaggat acgggccgct
    17401 caatgcgact ttccgggcgc gctgttttcc gttgacctct acggcggcaa gccggctgcc
    17461 ttcgattttc tgcgcaactt gaagatcgcc cggaatgccg tatcgctcgg cggcgtcgag
    17521 acgctcgcct gccacccgaa aaccaccacg cattcgtccg tgtctcagca ggaactcgaa
    17581 ctcgcgggcg tgaccgacgg cctggtgcga atctcggtgg gcatcgagga ctggcgggac
    17641 ctgctcgcgg atttcgagca ggcgctggat gccatcccgg cggaggccgg cgtaggacaa
    17701 ttgcgctccg gctcttgaaa gctggacctc gttctgtttt gtcggggctt gtttataatc
    17761 cccgacgttt cgtaaaataa agcatgccga ttgccgaacc tcttgccaag gttgctgcgc
    17821 tcgccaaaac cgcgggcgtt ctgcgtcccc gcgatctgga tgcgcatggc atcgcacgac
    17881 aatacctgcg actggcagaa cagcaaggtc tcgtagtccg gtcgggccgt ggactctaca
    17941 ctgcagcaga tgctccggtt accgaactcc acacgatcgc cgaaacttcg aagagagtcc
    18001 cacgcggtgt catctgcctg ctttctgcgc tgcggttcca cgacatcgga acgcagaacc
    18061 cgtttgaagt gtggatcgcg attggcgaga aggatcgccg ccctgccgct gagttccctc
    18121 ccctccgtgt ggttcgcttc tccaagcagt cgcttgcttt tggccaggag atccatgaca
    18181 tcgaaggagt accggcccgg atcttctccg ttgcgaagac cgtggctgat tgtttcaaat
    18241 accggaataa aatcggtctt gatgtcgcgc tggaagctct gcgtgagtgc ctcaaacaac
    18301 gcaaagcaac gaccgcggac ctgtgggcgg ccgccaacgt gtgccgggtg gcgaacgtca
    18361 tgaagccgta catggaggct ctcgtttgaa gcctgtcaag cctcgcaatt tagcggcttc
    18421 gatccgccag aagctcctca acactgccac gaagcaacgc gaagacttcg gtctggttct
    18481 cacccggtac gggctggagc ggcttctcta tcgtctgagc cagtccaggt attgcgatca
    18541 gttcgttttg aaaggcgcca tgctctttca gatctggacg aacacgcttc accgtcccac
    18601 acgggacctc gatctccttg ggtatgggaa tccatcaccg gaacgctgtc tcgcagtgtt
    18661 tcgtgaacta tgcggactcg ttgttcctga cgacggcctt gatttccccg ctgaggccat
    18721 cagtgtcgaa aagatcaaag aggatcagga atacgagggc attcgcgtca ggctactggc
    18781 gcggatggcg aatatccgca tccctcttca ggttgacgtt ggattcggtg atgcgctgcc
    18841 agcgcgcccc ggagtgctga actacccgac actcctgccc atgccggccg cgcagatcca
    18901 ggtctatccc atggaagccg taatcgcgga gaaactggag gccatggtgc atctcggtat
    18961 gctcaatagc cggatgaagg acttctttga tatctggttc ctggcacgaa cgttttcgtt
    19021 cgagtcaagt gctctcgctg acgcgattcg cataacattt gagcggcgcg gtactcagct
    19081 cgatccaagg ggcttcgacg ccttgactgc cgaattgtcg gtagatacct caaagctcat
    19141 tcaatggcaa gcgttcctta acaagggcaa cctggtggca ccggagagct tcgccggcgt
    19201 gattggtgcg atcttgggat tcgtctcatt tcctctgcac gcaagcatcg ctgaaactcg
    19261 aagaccggcg tcttggccgc ctggtgggcc ttggctcgaa gcgaaccgga aaaacttagg
    19321 atcagagtcg cccatcggcg tacctgacca ggaagtggga aagctatgaa gcccatcgaa
    19381 acactggatg tgcggagccg gccggagtgg agaaaatggc tggcaaaaaa tcacagctcg
    19441 cgatctgaga tctggcttgt attccacaag agccatacag gcaccagcac gctcagctac
    19501 gacgatagcg tcgaggaggc gctgtgcttt ggatggatcg acagcatcat caagcgcctg
    19561 gatgactccc ggtttgcacg gaagttcacg ccccgaaaaa cagacagcaa gtggtcgtcg
    19621 atcaatcgta agaggtatgc ggaccttcaa tcgcgaggac tattagcggc gccgggcctg
    19681 aatcgcgcac ctacgagccg gagcggcgat gcgccacccc gggtcccgtg ggcgataccg
    19741 tcgtacatgg aagaggcgtt agaagcgaat ccccgtgctg cgcagtattt cgaacaattg
    19801 gctccatcgt accggcgaac gtacatcggt tggatcgatt cggccaaacg tcaggaaacc
    19861 aaagaaaaac gtcttcgtga agcgctcaca cttttagcgg ccggcaaaaa actcggtttg
    19921 aaatgacgag act
//
LOCUS       DASTMK010000074         7902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_580056, whole genome shotgun sequence.
ACCESSION   DASTMK010000074 DASTMK010000000
VERSION     DASTMK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580056"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..3676
                     /locus_tag="VFQ79_02995"
     CDS             281..3676
                     /locus_tag="VFQ79_02995"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138644.1"
                     /translation="MIDGKFRWIALFFFFLAAWIPAAAQTSQITGQVTDSTGAAVPAA
                     KVSILSHQTGIQRDLTTNSQGYYTAALLPYGVYTVSVSVSGFQTSSRPDVPLQEGQTA
                     RLDFSLQVGQISEQIQVVGSAPLLETETPTMSTVVDRQRVVDMPINGRNILSLALLTP
                     GVRAIGEFGGLPVSSYDGSRLSIAGGAATVNNFMVDGIAAENFASGGMQISLSVDATE
                     EFRIITRNPSAEYGRTGGGVVNVISRSGTNEYHGTLYYFHRNKALNANDFFSNAANRP
                     RAPFVFNQYGITVGGPVKKDRTFFFFNWERFHQRTEARAFRTVPTERQRAGDFSQTLD
                     ALGRQVAIYDPESTREDPTRAGTFIRQPFQGNVIPQNRLSPVALAASRYYPAPNNPGL
                     ANTGVNNFYGQASAPLDKDIYGIKGDHYVTPLRRIAARFTYDNTFRGQPNFYYNIAEP
                     DASSLNFIRNSSVVSYTDSLRPTLLLEARAGLNRYYTPRITRSYGFDVSEINMPAALN
                     TQMQFPMFPRFNLTDVTTIGNGGGDHQVQANNSYSGSAAMTWITGGHTVKFGGEHRLY
                     HANNSQGAGALQFSFNRSFTTGPDRNVTATNAGHGYASFLLGAAASGTANRYQPITYQ
                     VRHFALFLQDDWKMTPRFTLSPGLRWEYEGPLTDRFNAISLFDPSIMTTVNGVALRGG
                     VMYPGVDGRSRGNRDASWTDFGPRLGFSWQVRDRTVVRGGYGIYYLPTSGTFVRAGQT
                     GFTITTNMLTSADGGLTPEETISNPFPTGIMEPVGSSGGAVTGLGTSITGNMFNLKRG
                     YSQQWNMNVQREFGNGWLIEIGYMGNRGVSLPANREFSYLPESALALGPALQELVTNP
                     YAGLVSSGPLSAARVQRGVLLQTYPQYLSATGMDSWANSIYHAGTLRVEKRFHNGFSL
                     LGSYTWSKLIDDNVGGGLNAFADSGSNSVQNWNDLRAERAVSTSNMPHRVVVSGTYVI
                     PTPRFESRILRGLVAGWQTNWIFTAQSGNTLSVSTTAPAYTGSRPNMIGDPGVDEPTI
                     TRWLDRSAFAQPAQFTYGNAPRNLPSTRSDALVRLDASFFKNIALTERITAQFRAEAF
                     NLANTPTFGNPATGVLNTNFGVVSSLAVNTIPRQLQMALKIYF"
     gene            3767..6175
                     /locus_tag="VFQ79_03000"
     CDS             3767..6175
                     /locus_tag="VFQ79_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0138645.1"
                     /translation="MRRRDFLSSAAAGPAVIVPVEATQAADDPESRIQREEHRFDVFV
                     AGGGMAGVCAAISAARHGARAGLVQDRPVFGGNSSSEIRVRPAGSARNNAWARETGVI
                     EEFMLRDLASNYAKCNSNWDLILYDAVRRQAGLTSFLNTRITEVKSAPTQNGHRRIEA
                     IHATQLATEKNFVFTADQYVDATGDATVGFLAGADFRYGAEPRSEFKEPLAPVRGGDV
                     SLGATIGLMSRNIGRPVPYEAPPWVETYRRPEDFGPGRRVSRGGDTDGWWWLSVGAPH
                     HQVYDTPEVRDELLRQALGIWNYIKNYSPDKAAAANYALEWVGMVPGKRESRRLMGDV
                     LLTEADCHQDRKWPDRVCYAGWDIDLHVRGNVLNRKDPPERAIIDAHYKHWITVAPFS
                     IPLRAFYSRNIDNLWMAGRDMSVTHVALGPVRVQQTTASHGQAVGTSAAYAFSHKLTP
                     RQTADPEGKHIRQVQQILLRNDVRIPGLRNGDVEDFARTATVKATSESPLAFGGVQPG
                     EFRAFTTPLAQVFPVTHGRIDTLELYMKNAGTTAELQVEIQELERIWDRTEGKTVATA
                     KVAMDANSEAWVPVPLNARVRPGRPYRCCVRGPEHVAWVQAAHYPTGTVAQFLHISPG
                     GIEAKNRHLPSFQLDEIDLPPYRHWITQRGIARSLKVAPAPLPYAAANVNNGVAWPID
                     MPNVWVSDPAAGLPQSVEVQFEGERTVDTVLVGFDTDLDPQTNTKPPFWKPSTCARHW
                     RLFVMAGGKWRNAYEEEDNYLRRRTATFDPIATTALKLEVLATNGDPSARVYEIRAYD
                     SRRI"
     gene            complement(6350..>7902)
                     /locus_tag="VFQ79_03005"
     CDS             complement(6350..>7902)
                     /locus_tag="VFQ79_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138646.1"
                     /translation="STGTYSESLRQRRLHAFLQDNYRVSRRLTLNLGMRFDPSVPWRE
                     IQARKITLFRPGLHSTRFPHLPTGAVVAGDPGVPEQGYASDLNNVSPRLGFAFDPVGS
                     GKTSIRGGAGFFFGQPISASINQRSQVSYPFSESLTITSPGSLGPGGVANVFRTGTAF
                     AIPNPYLGTAEQAAAPRPLTYVSTAAGTSLPYTVQWNLSVERQATREWIASVIYTGSK
                     STHLLMQHERNPPRYVPGTGQDGRPLSTHANIDARRPYGPHFGSVQEMGTDGNSNFNA
                     LTLKVRKTGGGHVWWSKSILEAHYTWSHTLDVLSTAVGPGGSSARDPLNERLDYANAD
                     FDHRHRFVLSWVFPLPRLAGFAAAPRRLLGGWNTSAIVTLQDGNPLTVLSGLDRNLDG
                     TTATDFADQILPNAALPENRPRADKIRQWFNTAAFTPNAPGTYGNTGRNILRGPSLAN
                     VDFALYKELYLGAKEERRLEIRSDYFNLFNRVNLGIPSMSASDPLFGRINSAGTPRII
                     QLALKLYF"
BASE COUNT         1568 a         2433 c         2428 g         1473 t
ORIGIN      
        1 atctgctgga tgcgccagtc gaattttcga atagcgcgga cttgttttgc ctcgaccttt
       61 acaggcactt cgatatcgaa cccctcacac aagccgccac gaagacccgc attgtgtgga
      121 gatgaacggg gatgccagac atccgcgcca ctacctgaaa aagcgcatcg acgcgccgcg
      181 gtgtgcggcg ggctagacgc atcggtctac gacaatacgc tggatttttg cggccgtgaa
      241 gatcactcgc ctaacctgac taaaggggta tgattgaacc atgattgacg gaaaattccg
      301 ctggattgct ctgtttttct tttttcttgc cgcatggatc cctgccgccg cgcagacttc
      361 gcagatcacg gggcaagtca ccgattcgac gggtgcggca gttcccgcgg ctaaagtctc
      421 gatactcagc caccaaaccg gcattcaacg ggatttgacc accaactccc agggttatta
      481 cacagcggcg ctgttgccat acggcgtcta caccgtttcg gtgtccgttt ccggatttca
      541 aacatcgagc cgaccggatg tccctctgca ggaaggacaa acggcgcggc tcgatttcag
      601 tttgcaggtc gggcaaatca gcgagcaaat ccaggtggtt ggatccgcgc cattgctgga
      661 aaccgagact ccgacaatga gtaccgtggt cgaccgccag agagtggtgg acatgccgat
      721 caacggccgc aacattctct cgctagcgct gctgactcca ggtgtgcggg ccatcgggga
      781 attcggtggt ctgccggtct cgagttacga cggttcccgc ctgtccatcg cgggcggagc
      841 ggccactgtc aacaacttca tggttgacgg catcgcggcg gagaattttg ccagtggtgg
      901 aatgcagatc agcctttcgg tggatgcgac cgaggagttc cgcatcatca ctcgtaatcc
      961 gagcgccgag tacggacgaa ccggtggggg tgtagtcaac gttatcagcc gctctggaac
     1021 gaacgagtac cacggcaccc tgtactactt ccaccgaaac aaggccctga acgcaaacga
     1081 cttcttcagc aatgccgcca accgtccgcg tgcgccattt gtgttcaacc agtacggcat
     1141 cacagtcggc ggaccggtga agaaagatcg caccttcttt ttcttcaact gggagcgctt
     1201 ccaccagcga accgaagcgc gggcgtttcg caccgtgccg acggaacggc agcgagcggg
     1261 cgacttctcc caaacgctcg atgccctcgg gcgtcaggtc gccatctacg atccggagag
     1321 cacaagggag gatccaaccc gtgcaggaac gttcatccgg cagcctttcc agggcaatgt
     1381 cattccgcaa aaccgcctga gcccggtggc gcttgcggca tcgagatact acccggcgcc
     1441 gaataatccg gggctcgcca acacgggagt caacaacttc tatggtcagg catcggcccc
     1501 gctcgataag gacatctacg gcatcaaagg ggatcattat gtaacgcctt tacggcgtat
     1561 cgcggcccgc ttcacgtacg acaacacctt ccgcggtcaa ccgaatttct actacaatat
     1621 cgcggagccg gacgcatcgt cgctgaactt catccggaat agctccgtcg tcagctatac
     1681 cgacagcctt cgtcccacgc tactgctcga agctcgcgcc gggctgaacc gttactacac
     1741 gccccgcatc actcgtagct acggcttcga cgtgtcggag atcaacatgc ctgcggcgct
     1801 gaacacccag atgcagtttc ccatgtttcc gcgattcaac ctgacggatg tgaccaccat
     1861 cggaaacggg ggcggcgatc accaggtgca ggcgaacaac tcctattcag ggtctgccgc
     1921 gatgacgtgg attaccggcg ggcataccgt gaaattcggt ggcgagcacc ggctttatca
     1981 tgcgaacaac agccagggcg cgggggcgct gcagttcagt ttcaaccgga gttttactac
     2041 cgggccggat cgcaatgtca cggccacgaa cgcaggacac ggctacgcca gcttcctgct
     2101 tggagccgcg gcgtccggca ccgctaatcg gtaccagcca attacgtacc aggtgcgtca
     2161 tttcgcgttg ttccttcagg acgactggaa gatgacgccg cgcttcactc tcagcccggg
     2221 actgcgctgg gagtacgaag gaccgctgac cgaccgcttc aacgccatca gcctcttcga
     2281 tccctcaatc atgaccacgg tcaatggcgt tgccctgcgg ggcggcgtca tgtacccggg
     2341 tgttgatggc cgttcacgcg gcaaccgcga cgcctcatgg acggacttcg gtcctcgact
     2401 gggcttctcc tggcaagtaa gggaccggac cgtggtgcgc ggcggctacg gcatttacta
     2461 cttgcccacc agcggcacgt tcgttcgagc ggggcagacc ggcttcacaa tcaccacgaa
     2521 tatgctcacc tcagccgacg gagggttgac gccggaggag accatttcga atccgtttcc
     2581 caccggaatc atggagcccg tcggaagctc cggcggcgcg gtcaccggcc tcggcacctc
     2641 tatcacaggc aacatgttca acctgaagcg cggatatagc cagcagtgga acatgaacgt
     2701 tcagcgggag ttcggcaacg ggtggctcat cgagattggc tacatgggca atcggggtgt
     2761 aagcctgccg gcaaaccgcg agttcagcta cctgcctgaa tccgccctcg ccctgggacc
     2821 cgcactccag gagcttgtta ctaaccccta cgccggtctt gtttcgtcgg gccctctcag
     2881 cgcggcgcgc gtacagcggg gagttctgct gcagacatat ccgcagtacc tcagtgccac
     2941 cggtatggat tcctgggcga actcgatcta ccatgccgga acgcttcggg tcgagaagcg
     3001 attccacaac ggattctcgc tgcttggttc gtatacctgg agcaagctga tcgacgacaa
     3061 cgtgggtggt ggcttgaacg cgttcgccga cagcggctcg aatagtgtgc agaactggaa
     3121 cgatctcaga gccgagcggg ccgtctccac cagcaatatg ccccatcgcg tcgtcgtctc
     3181 gggcacctac gtcattccca cgccccgctt cgaatcccgc attctgcgcg gactcgtcgc
     3241 cggctggcag accaactgga tcttcacggc gcaaagcggc aatacactat ccgtatctac
     3301 cactgcgccg gcatacaccg gttcccgtcc gaacatgatc ggcgaccctg gcgtggacga
     3361 gcccaccatc acccgctggc tggatcgtag cgcgtttgcc cagcctgccc agttcaccta
     3421 cggcaacgca cctcgcaatc tgccgtcgac tcgctcggat gcgctggtcc ggctggacgc
     3481 atcgtttttc aagaacatcg ccctgacgga gcgcatcacc gcgcagtttc gagcagaggc
     3541 cttcaacctg gcgaacacgc cgacgttcgg taacccggcg accggcgtat tgaacacgaa
     3601 cttcggcgtc gtatcgtcgc ttgcggtgaa tacgattcca cgacaactgc agatggcgtt
     3661 gaagatttac ttttgaggag ctcagaatag aaacaatgag cggcaggttc gccttgagtg
     3721 cgtatccgcc ggccaccaat cgtctcggga ggcatgcaca accgacatgc gacgcagaga
     3781 ttttctcagc agcgcggcgg caggtcccgc ggtaatagtg cctgtggaag caacccaagc
     3841 ggcagacgat ccggagtcgc gcattcaacg cgaagagcat cgctttgacg tgtttgtcgc
     3901 cggcggcggc atggcgggcg tctgcgcggc aatctccgcc gcgcggcacg gagccagagc
     3961 cggactcgtg caggaccggc cggtgtttgg tggcaactcg tcgagcgaga tccgcgtgcg
     4021 tcccgccggc tccgctcgca acaacgcctg ggccagggaa acgggcgtga tcgaggagtt
     4081 catgctccgc gacctcgctt ccaactacgc caagtgcaac agcaattggg acctgattct
     4141 ctatgacgcc gtgcggcggc aggcggggct tacgtcgttc ctcaacacgc gcattacgga
     4201 agtgaagtcg gcgcctacgc agaatggaca ccgccgcatc gaagcgattc atgccacgca
     4261 gcttgctacc gagaagaact tcgtcttcac tgccgatcag tacgtcgatg ctaccggcga
     4321 cgctacggtc ggctttctcg ccggcgcgga tttccgctac ggcgccgaac ctcgatcgga
     4381 gttcaaggaa ccgctcgccc ctgtgagggg cggcgacgtc tcgctgggcg caaccatcgg
     4441 actcatgtcc aggaacatcg ggcgccccgt gccgtatgag gcgcccccat gggtggagac
     4501 ctaccgccgc cccgaggatt tcggccccgg gcgccgtgta tcccgcggcg gcgacacaga
     4561 tggatggtgg tggctgtcgg taggcgctcc tcatcaccag gtgtatgaca cgcccgaggt
     4621 gcgcgacgaa ctactgcgcc aggcgctcgg catctggaat tacatcaaga actactcgcc
     4681 ggataaagcg gccgcggcca actacgcgtt ggagtgggtc ggcatggttc cggggaaacg
     4741 cgagagccgc cgcttgatgg gcgatgttct gctcaccgaa gccgattgcc accaggaccg
     4801 gaagtggccc gaccgcgtgt gttacgcagg ttgggacatc gatctgcatg tcaggggcaa
     4861 tgttctgaac cggaaggacc cgcccgaacg cgccatcatc gacgcgcact acaagcactg
     4921 gatcacggta gctccgttca gcatcccgtt gcgcgccttc tatagccgca atatcgacaa
     4981 tttgtggatg gccggacggg acatgagtgt tactcacgtt gcgctcggac cggtgcgagt
     5041 acaacaaact accgcctcgc acgggcaagc tgtcggaacg agcgcggcct acgcgttcag
     5101 tcacaagctg acgccgcgcc agaccgcgga tcccgagggc aagcacattc gccaagtgca
     5161 gcagatattg ctgcggaacg acgtccggat tcccggtttg aggaacggag acgtggagga
     5221 tttcgctcgg accgcgacgg tgaaagcgac cagcgaatcg ccactcgcat tcggcggcgt
     5281 tcagccaggt gagttccggg cgtttacgac gccgctcgcg caggttttcc cggtgacgca
     5341 cggccgcatc gatactctcg agctgtacat gaagaacgcc ggcaccacgg cggaactcca
     5401 ggtggaaatc caggaactcg aacgcatctg ggaccggacg gaaggcaaaa ccgtggcgac
     5461 ggctaaggta gcgatggatg cgaattcgga agcctgggtt cccgtcccgc tgaatgcgag
     5521 ggttcgaccc ggccggccgt accgttgttg tgtgcgcggg ccggagcacg tcgcctgggt
     5581 gcaggccgcg cattatccaa ctggcacggt ggcgcagttt ctgcatatct ctcccggcgg
     5641 aatcgaggcc aaaaaccgcc accttcccag ctttcagctt gacgaaatcg acttgcctcc
     5701 ctaccggcac tggattactc agagggggat cgcccgctcg ttgaaggttg ctccggctcc
     5761 gcttccctac gccgctgcga acgtaaacaa cggcgtagct tggccgatcg acatgccgaa
     5821 cgtgtgggta tcggaccccg cagccggact cccccagtcg gtggaggttc aattcgaagg
     5881 cgaacggacg gtcgacaccg tcctggtcgg tttcgacact gatctggatc cgcaaacgaa
     5941 cacgaaacct ccgttttgga aaccctcgac gtgtgcccgg cactggcgct tgtttgtaat
     6001 ggccggagga aaatggcgca atgcgtacga ggaggaagat aactacctcc gccgccgcac
     6061 ggcgacattc gatcccattg ctaccacagc tctgaagctc gaggtactgg ccaccaacgg
     6121 cgacccgtcc gcgagagtct atgaaatccg ggcctacgac tcgcgccgca tatgaatcgc
     6181 gctcgaagtc tccgcggatc tctgtgaatc ctttggcgct ggacctctgg ggagtcgtca
     6241 ccatgggata gactgaggaa gcgttgcatc tgctcaccat gccgcggccc cggatacctg
     6301 ctctggctgg aaggcgccgc gggcgcgtgg atgaaggcgc cccggcgact caaaaataaa
     6361 gcttgagcgc cagttgaatg atgcgaggag ttcccgccga gttgatgcgc ccgaagagcg
     6421 gatccgacgc cgacatggag ggtatgccca gattcacgcg gttgaagagg ttaaagtaat
     6481 ccgaccggat ctccagtcgt cgctcttctt tcgcgcccag gtagagttcc ttgtataggg
     6541 cgaagtccac gttggccaga ctgggtccgc ggagtatgtt gcggccggtg ttcccgtagg
     6601 tgcccggagc gttgggtgtg aaggccgcgg tgttgaacca ctggcgaatc ttgtcggcgc
     6661 gcgggcggtt ctcgggaagc gctgcgttcg gaaggatctg gtcggcgaag tcggtggcgg
     6721 tggtgccgtc gaggttgcgg tctagtccgg agagcacggt tagcggattt ccgtcctgca
     6781 gcgtcacgat ggccgacgtg ttccaccccc cgagcaggcg gcgcggcgcc gcggcgaagc
     6841 ccgccaggcg gggcagcggg aagacccacg agaggacgaa gcggtgccgg tgatcgaagt
     6901 ccgcattggc ataatcgagc cgctcattca acggatcgcg cgccgaagat cccccaggtc
     6961 cgaccgccgt ggatagcaca tccagcgtgt gggaccaagt gtagtgcgcc tccagaatcg
     7021 acttcgacca ccagacgtgg ccgccgcctg tcttgcggac tttgagagtc agcgcgttga
     7081 aattggagtt tccatcggtg cccatctcct gaaccgagcc gaagtgcggg ccatacggcc
     7141 ggcgcgcgtc gatgttcgca tgggtcgaaa gcggcctgcc atcctggccg gtgcccggaa
     7201 catagcgtgg cgggtttctc tcgtgctgca tcagcagatg cgtggacttc gatccggtgt
     7261 agatgaccga ggcgatccac tcccgcgtcg cctggcgctc cacgctgaga ttccactgca
     7321 cggtgtatgg cagggaggta cccgcggcgg tggagacgta agttagcggg cgcggcgcgg
     7381 cggcttgctc cgctgttccc aggtagggat tgggaatcgc gaatgcagtt ccggtgcgga
     7441 atacgttggc gacgccgccc ggccccagcg agcccggaga agtgatggtc agcgattcgc
     7501 tgaacgggta ggacacctgg ctgcgctggt tgatggaggc ggaaatgggc tgtccgaaga
     7561 aaaacccggc tcctccccga atcgaggtct ttccggatcc caccgggtcg aaggcaaacc
     7621 ccaggcgcgg agagacattg ttcagatcgc tcgcataccc ctgctccggg acgccgggat
     7681 cgcccgccac gacggccccc gtgggcaggt gggggaaccg tgtggaatgg aggccgggcc
     7741 ggaatagagt gatcttgcgc gcctggatct cccgccaagg cacagaagga tcgaagcgca
     7801 ttcccagatt cagggtcagc cgtcgcgaca cacggtagtt gtcctgcagg aacgcatgca
     7861 ggcgtctctg gcgaagcgac tcactgtacg tgccggtgga ga
//
LOCUS       DASTMK010000075         6975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_612311, whole genome shotgun sequence.
ACCESSION   DASTMK010000075 DASTMK010000000
VERSION     DASTMK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612311"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFQ79_03010"
     CDS             complement(<1..479)
                     /locus_tag="VFQ79_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0138647.1"
                     /translation="MLANTGAAKAAAELPRKLGLIDSISIVSGTMIGAGIFIVPASIA
                     RELPSATAILAVWVVAGIISFFGALAYAELGAMLPQSGGQYVYLRETYGSLTAFLCGW
                     AAFLIIQSGSIATVAVGLGIYATYLFPNTPGLRWAPAVAIMLFTFINYRGVKAGART"
     gene            570..1400
                     /locus_tag="VFQ79_03015"
     CDS             570..1400
                     /locus_tag="VFQ79_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEU0138648.1"
                     /translation="MDIYEEIVRLRRLGQKCALATIVEVNGSIPSYESAKILVREDGS
                     MTGTIGGGCVEAEVWSIACSVMETEKPQRLTFNLGQDAAYDNGLICGGQLEIFVEPLI
                     PPPAALIFGAGHISKSLARIAGVAGFETTIVDDRESFANRDRFPEAGAIFAGEYEEVF
                     PQLTVNGSTYIIIVTRGHRDDMRVLRWAVETPARYIAMIGSKRKVIGVIKELEKEGIP
                     RSDFEKIFAPMGLEIGAVTPEEIAVAVVAEMIAVRRNPGGNWRTISKSIFAAEKPVLK
                     "
     gene            1397..1990
                     /locus_tag="VFQ79_03020"
     CDS             1397..1990
                     /locus_tag="VFQ79_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU0138649.1"
                     /translation="MTATAVILSGGASSRMGYPKALLDYQGETFADRLISAFSDCCQP
                     VLLVVGADAALIKAGIRRGDEVRMVLNPEPEQGQFSSLQCALREVDEACDAVAFTPVD
                     YPSIQHSTIAQLMDALRADTKNNLFFIPRFEEERGHPVVFRASLIPEFLALGREAQAR
                     QIVHKYRDRTKYIDVGDPGITRDVDDREAYIRLVSGS"
     gene            1987..4362
                     /locus_tag="VFQ79_03025"
     CDS             1987..4362
                     /locus_tag="VFQ79_03025"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU0138650.1"
                     /translation="MKRPARRALLVLLAAVAGVIVLAPVLSADRFKERIRTGLERALM
                     RKVEIQGRAHFSILAGPGFSVDNVLISEDPSMGIEPFAHVGTLTARIRLRSLLYGRME
                     FSSLLLDEPSVNLVRMADGPWNVQPLLKRAITRGRPAGGTPPDIEVRTGRLNFKFGDT
                     KSVFYLSNADVDVYALSGNDGAFAVRFSGEPSRTDRAAQSLGRLIGRGQWHPGEKQPG
                     RLNVYLEWEKSPLAEVWMLGAGRDLGVHGHIASQARIEGVLPRLDVTGTMQVDEIHRW
                     DLRPARGQSWPISYRGTLDLASETLDVAMEDSSLPVSAHFRATGLLSAPALGTTITFR
                     QAPMAPAASIARHMGLPLPPDLELQGTLDGVIGYSGATGFQGRLAMMDTSASMPSAAP
                     VTLERADVVVSGRRVELLPATVEVGKGQPARVEGEYDGANRTAAVRIQTRSLAISDLK
                     STAGRLLGASSPPLLSLCVGGRWRGSIRHDWATGSEGAWSGNFDLLNTAIPISGLNSP
                     VRIASASAVLKKNHLLLTRIEGEEEGIAFRGEYRFDAKAEPAHRLSITIPEAEAAAVE
                     LALRPTLGRPEGFFARAFRFGRARLPDWLKNRNAQALVHIGRLTGTGTAASIEALRAD
                     VVWTGPAIELRDVRLRYAGGSLRGSGGVDLSRAEPGYKIKAHLNEIGWREGTVSGDLD
                     ISTSGTGPAVLRNLKSAGCLNAQSVEILRDPDIHALSGCFEFWTMDGVPRLELTSIKA
                     SSLNESFLGTGSLQPDGRLTVDLTSGPRQVRMAGTLNPWQFEVSTEKAERR"
     gene            4428..5507
                     /locus_tag="VFQ79_03030"
     CDS             4428..5507
                     /locus_tag="VFQ79_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138651.1"
                     /translation="MEFVPDASLTEALNDARATARDQLAAAWQLQLERVQEQLAFGWK
                     EHLDHVFEERFAELSSRVSESFRLEVAAEVEKAAGTTRRDVSDRLNESVRRMCKAANH
                     EEWTAAFLESSAAFSERIALFSVLGENLQLEGACGLADALPGDVQRISIPLGSAPAFA
                     NAVESSDTVAAMRSATEISKTFSDIVGEDETQRVYLFPVVNRQRVVAILYSEPRVNTG
                     SVSALELLSTIAGLSLENRTTGAGKVPGLVGIAAATTPVQPAGWSDLPPEDQALHLRA
                     QRFARVQVAEIRLHRAEAVRAGRRERQLYFAVKQAVDSARETFRREFVSASPTMVDYL
                     HMELVRTLANDEISVLGPDYPGPMV"
     gene            5461..>6975
                     /locus_tag="VFQ79_03035"
     CDS             5461..>6975
                     /locus_tag="VFQ79_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0138652.1"
                     /translation="MKFPCSVRTIRGQWFKPGIAALFLLLAADLHAQGGPALLRSFRE
                     KPTAANRQAVERYAAARTRTQAGALAYLALGIADLDRSDFASARTNLRKAREFLPRLK
                     DYTGYLLASAEFGVNDFDKVKDYLDPVWDHKPASPLVPKAALLAARADIARGKPGDAI
                     KILKKDFESLPQSAGLLALATALENTGDRADAAAAYQKVYYEYPLASEASQAQAAISR
                     LQNAMGAEFPPAMPQAMLERASALITARRYADGKRELERLTSQLAGAQRDLALVRIGA
                     ADYFAGETSPAYRYLASLKVEDPEADAERLYYLLSCARRTGNDARIAEAAEELGRRYP
                     KSPWRFSALLSAGNHYLLVNDASKYVPFYAQAARDFPDDERADYCHWKVTWNSYIRRK
                     ADAAGLLHEHLERYPLSEKANSALYFLGRMAEEAGDLASAKSFFREVAMRYPNTYYAV
                     LARERLEERTMRNVVASGAAVDLISRISLPKRVAQESFQPNSITKMRLERARELE"
BASE COUNT         1381 a         1951 c         2266 g         1377 t
ORIGIN      
        1 tgagtccgcg ctcccgcctt cactccacgg taattaatga aggtgaagag cataatcgca
       61 acagcaggtg cccaccgtaa tcccggtgtg tttggaaaca gataggtggc atagatgccc
      121 aatcccaccg cgacggtggc gatggagccg gattgaataa tcagaaaagc cgcccaaccg
      181 cacaggaatg cagtcaatga gccgtacgtt tcccgcaggt agacatattg gccgcccgat
      241 tgaggcagca tggcgccaag ttcggcatag gccagcgcac cgaaaaacga gatgattccg
      301 gcgacgaccc acaccgccag aatcgccgta gccgaaggaa gttcacgggc aatggaagca
      361 ggcacgatga aaatgcccgc cccaatcatg gttcccgaca cgatcgaaat agaatcgatc
      421 aatcccagtt tgcgtggaag ttccgccgcc gctttggccg ccccagtatt cgcgagcatc
      481 tttcccggtc cgctgatccg cctattctag cgcgagtgcc catagacacc tgcttcgttg
      541 tacactttgg cagtaaaata ggatctctgg tggatatcta cgaagaaatc gtacgccttc
      601 gccgtcttgg gcagaagtgt gccttggcta ccatcgtgga agtcaacggc tccattccca
      661 gctacgagag tgcaaaaatc ctcgtgcggg aggacggctc catgacaggc acgattggcg
      721 gcggctgcgt ggaggcggag gtctggagta tcgcatgcag cgtaatggaa acggagaaac
      781 cgcagcggct tacgtttaac ctcggccagg atgccgcgta tgacaacggc ctgatttgcg
      841 gcgggcagtt ggaaatcttt gtggaacctc tcatcccgcc gccggcggcg ttgattttcg
      901 gagccggaca tatttcgaag agcctcgcca ggatcgccgg tgttgcggga ttcgaaacca
      961 ccatcgtcga cgaccgcgaa tcctttgcca accgggaccg gttccccgag gcaggcgcca
     1021 ttttcgccgg tgagtatgaa gaggtgtttc cgcaactcac tgtgaacggt tcgacgtata
     1081 tcatcatcgt gacgcgcggg caccgcgacg acatgcgcgt tttgcgatgg gcggtagaga
     1141 cccccgcgcg ttacatcgcc atgatcggaa gcaagcggaa ggttatcggc gtcatcaaag
     1201 agctggaaaa ggaaggtatt ccgcgctcgg acttcgagaa gattttcgcc cctatggggt
     1261 tggagatcgg cgccgtcaca ccggaggaga tcgccgtcgc cgttgtcgcg gagatgattg
     1321 ccgttcgccg caacccgggt ggaaactggc gcacgatctc aaagtcgatt tttgcggcgg
     1381 agaaaccggt attgaagtga ctgccacggc ggtcattctc tccggcggcg cgtcgagccg
     1441 gatgggatat ccgaaggcgc tgctcgatta ccagggtgaa accttcgcgg accgcctgat
     1501 ttcggcattt tcggattgtt gccaaccggt gctgctggtg gtgggtgccg acgccgcgct
     1561 catcaaagct ggaatccgcc gtggcgatga ggtgcgcatg gtgctgaatc cggagcccga
     1621 gcagggacaa ttcagttcac ttcagtgcgc tctgcgggaa gtggacgagg catgcgatgc
     1681 ggttgcgttt actcccgtgg actatccttc catccagcat tccactattg cgcaactgat
     1741 ggatgcgctc cgcgccgaca caaagaacaa cctgtttttt atcccaaggt tcgaagagga
     1801 gcgggggcat ccggtggtat tccgcgcgtc cctgattccg gagtttctcg cgctcgggag
     1861 ggaagcgcag gcaaggcaga tcgtgcataa gtatcgggat cggacgaagt atatagacgt
     1921 gggcgacccc ggtatcacgc gtgatgtcga cgacagggag gcgtatatac gtctggtgag
     1981 cggatcgtga aacggcccgc acgccgtgcg ttgctggtgc tgctcgcggc ggttgccgga
     2041 gtaattgtgc tggctccagt gctcagcgcg gatcgcttca aggagcggat tcgcacaggt
     2101 ctcgagcgcg cgctgatgcg gaaagtggag atccagggcc gggcccactt cagcatcctg
     2161 gcgggtccgg gcttcagcgt cgataatgtg ctgatctccg aagaccccag catgggaatc
     2221 gaaccgttcg cccatgtcgg cacactcacc gcgcggattc gcctgcgcag cttgctgtac
     2281 gggcgaatgg agttttcaag tctccttctg gatgagccga gtgtgaatct ggtgcgtatg
     2341 gcggatgggc catggaacgt tcagcctctg ctgaaacgcg cgattacgcg cggaaggccg
     2401 gcaggcggga cgccgcccga catcgaggtg cgaacggggc ggctgaactt caagttcggc
     2461 gacaccaagt cggttttcta tcttagcaac gcggacgtgg atgtatatgc gctctccgga
     2521 aacgacggag ccttcgccgt gcgattttcc ggcgaaccct cgcgcaccga ccgcgccgcg
     2581 cagagcctcg gccggctgat agggcggggg caatggcatc ccggagagaa gcagcccggc
     2641 cggttgaacg tctatctgga atgggagaag agtcctctgg ctgaagtgtg gatgctcgga
     2701 gccggacgtg atttgggggt ccacggacac atcgcttccc aggcgcgcat cgaaggtgtt
     2761 ctgccgcgcc tcgacgtgac aggaactatg caggtcgatg aaatccaccg gtgggacctg
     2821 agacccgctc gtgggcagtc ctggcccatc agctaccggg ggacgttgga tctcgcttcc
     2881 gaaactctgg atgtggccat ggaagattcg agtctgccgg tatcggcgca cttccgcgca
     2941 acggggttgt tgtcggctcc ggctcttggc acgaccatca ctttcagaca ggcgcctatg
     3001 gctcccgcgg cgtccattgc acgccacatg gggctgccgc tgcctccgga tctcgagctt
     3061 caggggacat tggatggtgt gatcggctac tccggagcga ccggttttca agggcgcctg
     3121 gcaatgatgg acacttctgc ctccatgcct tcagcggcgc cggtcacgtt ggaacgtgcg
     3181 gatgtagtgg taagcgggcg gcgggtcgag ttgctcccgg caaccgtgga ggttgggaaa
     3241 gggcagccgg cgcgcgtcga gggcgagtac gatggtgcga accggacggc agccgtccgg
     3301 attcagacgc ggtcgcttgc catctcggat cttaaatcta ccgccgggcg gcttctgggc
     3361 gcgagttccc cccctctgct gagtctttgc gtaggcggcc ggtggcgcgg ttcgatccgc
     3421 cacgattggg caaccgggag tgaaggagca tggtccggga atttcgatct gctgaacact
     3481 gcgatcccga tctccgggtt gaactcccct gttcggattg cttccgcatc ggcggtgctg
     3541 aagaagaatc atcttctgtt gacccggata gagggcgagg aggaggggat cgcgtttcgc
     3601 ggggaatacc ggttcgacgc caaagccgaa ccagcgcacc ggctgagcat cacgattccg
     3661 gaagcggagg cggctgccgt ggagcttgcg ctgaggccca cgttgggccg gccggaaggg
     3721 tttttcgcaa gggcgttccg ttttggccgc gccagattgc cggattggtt gaagaaccgg
     3781 aacgcccagg cgctggttca catcggccgc ctgacaggaa caggaaccgc cgcgtccatc
     3841 gaagccctgc gggcggacgt cgtttggacc gggccggcga tcgaattgag ggacgtccgc
     3901 ctgcgctatg caggtggcag cctgaggggt tcgggtggag tggatctaag ccgggcggag
     3961 cctgggtaca aaatcaaggc ccacctgaat gaaatcggct ggcgcgaagg caccgttagc
     4021 ggcgacctcg atatcagtac atccggtacc ggtccggctg tgctccggaa tctgaagtcc
     4081 gcgggctgct tgaatgcgca atcggtggag attctccgtg acccggatat ccacgcgctt
     4141 tccggctgtt tcgaattctg gacgatggac ggggttccgc gcctggaact cacgtcgatt
     4201 aaagcgtcgt cgctgaacga gagctttctc gggacgggct cgctgcagcc cgacggacgg
     4261 ctgaccgtgg atttgaccag cggccccagg caagtgcgaa tggcggggac gttgaatccc
     4321 tggcagttcg aagtgagcac ggaaaaagca gagcgccggt gatgtttcat catgttcttt
     4381 atgaggagca tcgaaaatga accgcgctac aatggtgttg tctgctcatg gagttcgttc
     4441 ccgatgcgtc tcttactgaa gctctgaacg atgcgcgggc tacggcgcgg gaccagcttg
     4501 ctgcagcgtg gcagctccaa ttggaacgcg ttcaggaaca gcttgccttc ggttggaaag
     4561 agcatctcga tcacgtgttc gaagagcggt tcgccgaact gtcttcgagg gtctcggaaa
     4621 gctttcggtt ggaggtcgcg gctgaggttg agaaggccgc gggtaccacc cgccgggacg
     4681 tcagcgaccg cttgaacgag tccgtgcgca ggatgtgcaa ggccgccaat cacgaggagt
     4741 ggacggcggc attcctggaa tcgagtgcgg cgttctccga acgcattgct ctgttcagtg
     4801 tcctgggcga gaacctgcaa ctcgaagggg cgtgcgggtt ggcagatgct ttgcccggcg
     4861 atgtgcagcg gatttccata ccactgggat cggctccggc attcgcaaac gccgtagaat
     4921 cgtccgatac ggtggcggcg atgcggagcg ccaccgagat ttccaaaaca ttttcggaca
     4981 ttgttggcga ggatgaaacg cagagagtgt acctgtttcc cgtcgtcaac cgtcagaggg
     5041 tagtagcaat attgtattcc gaaccgcggg tgaacacggg gagcgtgagc gctcttgagc
     5101 tcctctccac gatagcgggc ttgtcgctgg agaatagaac gacgggagcg ggaaaggtgc
     5161 cgggcttggt gggaatcgct gcggcgacga ctccggtgca accggcagga tggtctgatt
     5221 tgcctccgga agatcaggcg ctgcacctga gagcacagcg gttcgcgcga gtacaagtgg
     5281 cggagatacg gctgcaccgg gcagaggccg tcagagccgg acgccgggag cgccaattgt
     5341 atttcgcagt gaagcaggcg gtcgattccg cccgtgaaac attcaggcgc gaatttgtga
     5401 gcgcctcgcc gacaatggtt gattatctcc atatggagct ggtgcgtact cttgccaacg
     5461 atgaaatttc cgtgctcggt ccggactatc cggggccaat ggtttaagcc cggcatagcc
     5521 gcgctgtttc ttttgctcgc cgcagatctt catgcgcagg gcggcccggc cctgcttcgt
     5581 tccttccgcg aaaaaccgac agcggcaaac cgtcaggctg tagaacgcta tgccgcggcg
     5641 cgaaccagga cccaggcggg cgcactggca tatcttgcgc tgggcattgc ggacctcgat
     5701 cggtccgatt tcgcgtccgc gcggaccaat cttcgtaagg caagggaatt tctgccgcgc
     5761 ctcaaggact acacaggcta tctgctggca tcggcggagt tcggtgtaaa cgatttcgac
     5821 aaagtgaaag attatctgga tccggtttgg gaccacaagc ctgcttcccc acttgtcccg
     5881 aaagcggcgt tactggctgc cagggcggac atcgcgcgcg gcaagccggg cgatgcgatc
     5941 aagatcctga aaaaagattt cgaatctctg ccgcaatcgg cagggctcct tgcactggcg
     6001 acagccctcg agaatacagg cgatagggcg gatgcggccg ccgcatacca gaaggtttat
     6061 tacgagtatc ctctcgcctc cgaagccagt caggcacaag ccgccatttc gcggctccaa
     6121 aacgcgatgg gcgccgaatt tccgcccgcg atgccccagg caatgctgga gcgggcaagt
     6181 gctctcatta ccgcacggcg ctacgcggac ggcaagcgcg aactggaaag gctcacgtct
     6241 caattggcgg gagcgcagcg cgatctggcg ctggttcgta ttggcgcggc ggattatttc
     6301 gcgggagaga ccagcccggc ctaccggtat ctggcgagcc tgaaagtaga ggatccggaa
     6361 gcggacgccg agcggctcta ttacctgctt tcctgtgcgc gccggaccgg aaacgatgcc
     6421 cggatcgcag aggctgcgga ggaattgggc cgtcgctatc cgaaatcccc atggcgtttt
     6481 tccgcactgc tgagcgcggg aaaccactac ctgctggtga acgacgcttc aaagtacgtg
     6541 ccgttctacg ctcaagctgc acgcgatttc ccggacgacg agcgggcgga ttattgccac
     6601 tggaaagtga cctggaattc ctatatccgc cggaaggcgg atgccgccgg tctgttgcat
     6661 gagcacctgg aacgatatcc tctctctgaa aaagccaatt cggcgctgta ttttctcgga
     6721 cgcatggcag aggaagccgg ggacctggcc tcggcgaagt cgttcttccg ggaagtggca
     6781 atgcgatacc cgaatacgta ttacgcggtg ctggccaggg agcgtctgga agagcgcacc
     6841 atgcggaacg tggtcgcttc gggcgcggct gtcgatctga tttcgcgcat ctcgttaccc
     6901 aaacgcgtgg cgcaggagag ttttcagccg aactccatca ccaagatgcg tctggagcgc
     6961 gcgcgggaat tggag
//
LOCUS       DASTMK010000076         8936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_323888, whole genome shotgun sequence.
ACCESSION   DASTMK010000076 DASTMK010000000
VERSION     DASTMK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323888"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1359)
                     /gene="recA"
                     /locus_tag="VFQ79_03040"
     CDS             complement(346..1359)
                     /gene="recA"
                     /locus_tag="VFQ79_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU0138653.1"
                     /translation="MDGERRRAIEQAMSQVDRQFGKGSVLRLGSRNALPVSVIPAGAI
                     SIDAALGVGGFPRGRVIEIFGPESSGKTTIALQVLAEAQKAGGTVAFIDAEHALDPLY
                     ARNLGVDVDNLLVSQPDYGEQALEIMAALVACGAVDAVVLDSVAALVPKAELEGEMGD
                     SFMGLHARLMSQALRKLTGMVARTNTCLIFINQVREKIGVMFGNPETTTGGRALKFYA
                     SVRAEVRRLAAIKDGDKTVGNRTKIKIVKNKVAAPFREAEVDILYGRGVSREGDLLDL
                     GAANNLVEKSGSWFSFRGERLGQGRENARLFLQNNPALRNRIEAEIRKHLGLAAASEG
                     AAA"
     gene            1450..2715
                     /locus_tag="VFQ79_03045"
     CDS             1450..2715
                     /locus_tag="VFQ79_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU0138654.1"
                     /translation="MSAKRRIVLFAATTGYQVRVFADVAQRLGFDLTLATDRCHVLDD
                     PWGDHAVAVRFDKPGPSLKALQGPFDGVVAVGDRPTLLAAAAAERFGVRYHPVRAVEA
                     ASDKFLARERFRAAGLRLPSYCRIPLSGDAAASASTSYPCVLKPLGLSASRGVIRADS
                     PEQFVAAFERIRSILEQPDIVRRRAAQDQFLQVESYIPGSEYALEGLVTKGRLQTLAL
                     FDKPDPLDGPFFEETIYITPSREPGPVQNELIETAARAVEALGLTDGPVHAEMRHNDR
                     GAWILEVAARPIGGLCAKALRFRGGMGLEELVLRHSVGEDVSGVALSEEASGVMMIPI
                     PREGIYESVSGVEEAQAVAGVEDVIITAKEGQKLLPLPEGSSYLGFLFARAASPEAVE
                     TALRQSHARLEFQLATTLPVLRAPAARGR"
     gene            complement(2731..3462)
                     /locus_tag="VFQ79_03050"
     CDS             complement(2731..3462)
                     /locus_tag="VFQ79_03050"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEU0138655.1"
                     /translation="MQTLRVISLLIAALPLGAQSQPQYDIYRAPSAIAIDGKLDEAAW
                     QQAPPAGDFNFNWWKEGAKEQTVVKLLWDDRNLYLGYYCHDKHISATVTERHGPVSRD
                     DCVEVFLSPNPEKTRNYYGFEINVIGTMLNFIRADWYKGPFHWEPEGVRYRTSFHGLP
                     EKRESPDDDHWILELAIPFTNFEKDAAHTPPLDGDTWRLNLNRAGGITNAQYSTWSPV
                     SSPKPNFHVPESFGRVRFVNRAAGK"
     gene            3642..3983
                     /locus_tag="VFQ79_03055"
     CDS             3642..3983
                     /locus_tag="VFQ79_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0138656.1"
                     /translation="MGRPQKPGAEAIGILDLLILSRLARGSMHGFGIAEYLHNVSDAL
                     HVEEGSLYPALHRLESQGLIESEWGVSENNRRARFYRLTPRGRKQIRVEVSNWRRLVA
                     AVNQVIDPLVP"
     gene            3983..5149
                     /locus_tag="VFQ79_03060"
     CDS             3983..5149
                     /locus_tag="VFQ79_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138657.1"
                     /translation="MLKQFLIDVRVRLAALLARRAIHERADEEVQFHLSMIEQRMIKS
                     GMPPEIARMQARREFGNPTLIREQTADSWRYASVDSLIQDVRYGLRLFGRKPGFTAIV
                     ALTLALGIGANTAVFSIVEAVLLRPLPYRDPSRLVVIYLRNVHETGTSKMFASLRDYR
                     VFARAHSFEQAAAATWATGGRLLRGYGQAQDILAMPVSESFFALLGVEPALGRTFIPG
                     GVQGGCSVVLSDRLWRGPLRGDGAIIGSSIELDDRSCTVLGVMPPSFAFYPTAAQAWM
                     VLTPDFSPPPDQIPIGIFARLRPGVTIAQAQAEVSALHTALHRSDGQERDLAPLVADL
                     HGEFTFLAEARLRASANWLCAPRSGAGAPGSHASCLRKGCCSLRLAVRPGLLWP"
     gene            5041..6501
                     /locus_tag="VFQ79_03065"
     CDS             5041..6501
                     /locus_tag="VFQ79_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0138658.1"
                     /translation="MRAALGCGRARLARQLLTEGLLLATVGGAAGIAVALAAIHYFRV
                     AAPIEMPPGADVRMSWPVLAFTAVVSLATALLFGALPAWRASRAVESLQAGRSSAQAT
                     PHRVMKTLIAAEMALSLVLLAGSGLLMQSVLRLGSEQLGFESRGLVTARVKLPVEDYQ
                     KPGRRVQFYDRVLEALGNKAALSTGLPPHGWSGLATLHIPGREPSGSHDAGYRSVSPR
                     YFRVMGERLLRGREFNDRDRASSEPVVIVNEALARRYFANADPIGQRIALNEPSETNP
                     WRVIVGVVADEKGWGGFDRMGWAVMPGAFKPLAQDVPRSAALVARGSAADLRRAVTGI
                     DETVAIGEVEAMDAGLDRVLAYPRFRAALLSAFSLFAVLLAAIGLYGMLGQFVVQRTP
                     EIGIRMAMGARPADVLRLVACQAGAPLTAGLALGLAGALALSRSLASVLYGVQAGDPA
                     TFAAVSLALIAAGAIAAFFPARRAARVDPMTALRNE"
     gene            complement(6532..6828)
                     /locus_tag="VFQ79_03070"
     CDS             complement(6532..6828)
                     /locus_tag="VFQ79_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEU0138659.1"
                     /translation="MDAEKPGGRKIFCVKFQREMAGLDEPPFDNELGQRIYENVSKEA
                     WRLWVEQMKMIMNEYRLNLGNPDAQEFLMKQMEEYFWGAGAAFPPDYVPPRSKA"
     gene            complement(6928..7272)
                     /locus_tag="VFQ79_03075"
     CDS             complement(6928..7272)
                     /locus_tag="VFQ79_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138660.1"
                     /translation="MPEREIADEILGMLGREPDSDAILQQFNKLIQELLRGNMKRNSF
                     RRWEIDLLLDIGDCNLQGSQRRNLLQRYQKAVQRDMEKGASTPFKLSEYLEMLRARRD
                     AREERRPENGAG"
     gene            7290..>8936
                     /locus_tag="VFQ79_03080"
     CDS             7290..>8936
                     /locus_tag="VFQ79_03080"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEU0138661.1"
                     /translation="MGSDTSGPRDASVRPAAVERLFEFSLLGLLTSGYLAVAGSGALD
                     VPTLAATGIAIVLRALMAAGVLRLRISDTAVTVATLCYIAFYPLDYFFLSREFLAATV
                     HLVFFLLVVKVLTARTTRDYVFVKIVAFLELLAASVLSTSLSFFVFLAFFLVFGVATF
                     TSSEIRRSTGKRVATSRAGLRSIHIRLTALSLVIAMGILGMTAGLFFLLPRTARAAFQ
                     HFASQKYRLPGFSNEVTLGEIGEIKRQSTPVMHVRIEGNRSQLYLKWRGAALAEFDGK
                     RWYNSNNGGGEIVKVEKGIATLVQNPQRWRIGPRISYNVILKSIASDTLFLAGTPEFL
                     QVQARTVIRTPTDSYQLGYGAGDGMRYIGHSFLGSGPTPEPAAPLTPAARNLYTSLPG
                     PDARISQYAAKIASGLRSDGERARAIENELRTQYGYTMELPEKELADPISDFLFRRRK
                     GHCEYFASSMAVILRTLKIPARVVTGFQSGLYNPISGWYVIRASDAHSWVEAYLPGQG
                     WTTFDPTPPDPGAGAISAWTRLMLYFDAADIFWQEWVLNYD"
BASE COUNT         1528 a         2722 c         2809 g         1877 t
ORIGIN      
        1 gcggagtcgc acagcgcttt ccgctccggc tgggcatggc aaccggccgt ctgatcgagc
       61 gagccgattg gcgcgcactt cgctgggact cgtctacctc agctattgtg ccttcctttg
      121 cgttggatgc agcctgggca aacgtgtcca tgcgcgtgtt tgctgtttaa tgataaggga
      181 tgccggaagc cggtgtaaag agaattccgc taaaaatggc cgatttttaa ggttttttgc
      241 tcacgggagg cgctgggacc cccataaaca ggggtttagg acgccaaaac atttaggctg
      301 gccgatgttt tggtgatttt gagccccggc ggcgtgtttc cgctcctacg ccgcggcccc
      361 ttcggaagcc gccgccaagc cgagatgctt ccggatttct gcctcgatgc ggttccggag
      421 agcggggttg ttctgaagaa acagcctcgc gttttcgcgg ccctggccca gccgttcgcc
      481 gcgaaagctg aaccacgatc cgctcttctc caccaggttg ttggcggcac ccagatccag
      541 caggtcgccc tcgcgggaga cgccgcggcc atagaggatg tccacttccg cttcacggaa
      601 tggtgccgct accttgttct tgacgatctt gatttttgtc cggttgccga cggtcttgtc
      661 tccgtccttg atcgccgcca gacggcgcac ttccgcccgg acggaagcat agaatttcag
      721 ggctcggccg ccggtggtgg tctccggatt gccaaacata acgccgatct tctcgcggac
      781 ctgattgatg aagatgaggc aggtattcgt gcgggcaacc atgccggtaa gctttcgaag
      841 cgcctgcgac atcagtcgcg catgcagtcc catgaagctg tcgcccatct ccccttcgag
      901 ctccgctttg ggaaccagcg ccgccaccga atcgagaacg acagcatcca cggctccgca
      961 ggcaaccagg gccgccatga tttccagcgc ctgctcgccg tagtcgggtt gtgagaccag
     1021 caggttgtcg acatccacac ccagattgcg ggcgtaaagg ggatcgagcg cgtgttcggc
     1081 gtcgatgaac gccaccgtgc ccccggcctt ctgtgcttcc gccagcacct gtagcgcgat
     1141 ggtcgttttg ccggacgact cgggaccgaa gatctcaatc acccgtcctc tgggaaagcc
     1201 gcccactccc agggcggcat cgatcgaaat cgccccggcc ggaatcacgg aaacgggcag
     1261 cgcattcctg gatcccaggc gaagaaccga accttttccg aattgcctgt cgacttggct
     1321 catggcctgc tcgatcgccc gccgccgctc accgtccatt cgcagattcc tcctgcaacg
     1381 gagttggtag cttgagatac ccgttcgtct cagtcaaagg cggaatcgag ggtaagatcg
     1441 gaattgggaa tgagcgcgaa aagaaggatt gtgctgttcg ccgcgacaac cggataccag
     1501 gtccgtgtat ttgctgatgt ggcgcagcgt ctgggtttcg atctgactct ggcgacggat
     1561 cgctgtcacg ttctcgacga tccgtggggc gatcacgcgg tagcggtgcg tttcgacaag
     1621 cccggcccgt cgctcaaagc gctgcaggga ccgtttgacg gcgtcgttgc ggtgggcgac
     1681 cgtcctacgc tgctggcggc cgcggcggcg gagcgtttcg gcgtccggta ccacccggtt
     1741 cgcgcggtcg aagcggcgtc ggacaagttt ctggcgcgag agcgtttccg ggccgcgggc
     1801 ttgcgccttc cttcctactg ccgcattccg ctctcgggcg atgccgccgc ttccgcgagt
     1861 acatcctatc cctgcgtttt gaagcctctg ggcctctccg ccagccgcgg cgttatccgt
     1921 gccgattccc cggagcagtt cgtggcggct ttcgagcgga ttcgatccat tctcgagcag
     1981 ccggacatcg tgcgccggcg cgccgctcag gatcagttcc ttcaggtgga gagctatata
     2041 ccgggctccg aatatgcgct ggaggggctg gtgacgaaag ggcggctgca aacgcttgcg
     2101 ctgttcgata agccggatcc gctcgacggg cctttcttcg aggaaaccat ttacattacg
     2161 ccttcccggg agcccggacc cgtccaaaac gaactcatcg aaacggcggc gcgtgccgtc
     2221 gaagccctgg gcctcacgga cggtccggtt cacgccgaga tgcgccacaa cgatcggggc
     2281 gcctggattc tcgaagtagc ggcgcggccc atcgggggac tgtgcgccaa ggccctgcga
     2341 ttccgcgggg ggatgggcct cgaagaactc gtgttgcggc attcggtggg cgaggacgtt
     2401 tccggggtcg ctctttccga agaggcctcc ggcgtcatga tgattcccat tccgcgcgaa
     2461 ggcatttacg aatccgtgag cggcgtggaa gaagcccaag ctgtggcggg cgtcgaggat
     2521 gtgatcatca ccgccaaaga gggccagaag cttctgccgt tgcccgaagg cagcagctac
     2581 ctggggttcc ttttcgctcg cgcggccagc ccggaagcgg tggagacggc gctgcgccag
     2641 tcgcacgcca gactcgaatt tcagcttgcg actacgctgc cggtgctgcg ggcgcctgcc
     2701 gcgcgcgggc gttgagagtt gggccggctc tcattttccc gccgcgcggt tcacaaaccg
     2761 cacccggccg aaactttccg ggacgtggaa gttcggcttg gggctcgaca cgggcgacca
     2821 cgtgctgtac tgcgcgttgg tgatgccgcc cgcgcgattc aggttcagtc tccaggtgtc
     2881 gccgtcgagg ggaggcgtgt gcgcggcatc tttttcgaag ttcgtgaacg ggattgccag
     2941 ctcgaggatc cagtgatcgt cgtccggcga ctcccgcttc tccggaagcc cgtggaagga
     3001 ggtgcgatag cgcactcctt ccggctccca gtggaacggc cctttatacc aatctgcacg
     3061 aatgaaattg agcatcgtac caatgacgtt gatctcgaag ccatagtaat tccgggtctt
     3121 ctccgggttc gggctgagga agacttcaac gcaatcgtcc cgtgagaccg ggccatgacg
     3181 ctcggtaacc gtggccgata tgtgcttgtc gtggcagtag tagccgaggt acaggttcct
     3241 gtcgtcccaa agcagcttca cgaccgtctg ttctttggcc ccctccttcc accagttgaa
     3301 gttgaaatcc ccggcggggg gagcctgctg ccatgcggcc tcgtccagct tgccgtcgat
     3361 cgcgatggcc gacggcgcgc gatagatgtc gtattgaggc tgcgattgcg cgccgagcgg
     3421 caatgccgca atcaggaggg agattacccg gagcgtctgc accggagtag tatatcgaat
     3481 cgcttcactt ccgcacgccg atcatccaaa agaagaaagc gagcacggcc tttctgacct
     3541 acagggccgc ctggatggtc atcgagagcc gcgactggtg atggcggcac gtcgcggttg
     3601 acacgccggg cggtattggt gcatacccat atcaaaaatc tatgggtagg ccacaaaagc
     3661 ccggtgcaga ggcgataggg attctggatc tgctcattct ttcccgcttg gctcgcggat
     3721 ccatgcatgg attcgggatc gcggagtatc ttcataatgt ttcggatgct ttgcacgtcg
     3781 aagagggatc tctgtacccg gccctccacc ggcttgagtc gcaaggcctg attgagtccg
     3841 agtggggagt ttctgagaac aaccgccgcg cgcgatttta ccggctgact ccgcgcgggc
     3901 ggaagcagat ccgtgtcgag gtgtcgaact ggcggcgtct ggtggccgcc gtcaatcaag
     3961 ttattgatcc cctggtaccg tgatgctcaa gcagtttttg attgacgtac gggttcggct
     4021 tgctgctctg cttgcccggc gtgcaatcca tgagcgggcc gatgaagagg tgcaattcca
     4081 tctctcaatg atcgagcagc gcatgatcaa gtccggcatg ccacccgaga tcgctcgtat
     4141 gcaagctcga cgcgagttcg ggaaccccac actcatcagg gaacaaacgg ccgattcctg
     4201 gaggtatgca tccgtggatt cgctcattca ggatgtccgc tacgggttgc ggctgtttgg
     4261 gcgtaagccc ggcttcacgg cgattgtggc gctgacgctc gcgctcggga tcggcgcaaa
     4321 cacggctgtt ttcagcatcg tggaggccgt actgctgcgg cctctgccgt acagagaccc
     4381 ttcacgtctg gtcgtcatct acctgcgcaa cgtgcacgaa accggcacat cgaagatgtt
     4441 cgccagtttg cgggactatc gcgtattcgc gcgcgcccac agcttcgagc aggcagcagc
     4501 ggcgacatgg gccacaggag gccgtctcct gcggggatat gggcaggcgc aggacattct
     4561 ggcgatgcct gtgagcgaat cgttctttgc cttgcttggt gtcgaacccg cgctcggccg
     4621 tacgtttata ccggggggcg tgcagggcgg ctgctcggta gttctcagcg accggctgtg
     4681 gcgcggccct ctccgcgggg atggcgcgat tatcggcagt agcatcgaac tcgacgaccg
     4741 ctcctgcacc gtgctcggag tgatgccgcc ttccttcgcg ttttatccca ctgcggcaca
     4801 agcctggatg gtgctgaccc cggacttctc gccgccgcct gaccagatcc cgatcggcat
     4861 ctttgcgcgt cttcgccctg gcgtgacgat cgcgcaggcc caggctgaag tctcggctct
     4921 gcacacggca ctccaccgta gcgatggcca ggaacgcgac ctggcgcccc tggtggccga
     4981 tctacatggc gaattcacgt ttcttgcgga agcacggctg cgcgccagcg cgaattggct
     5041 gtgcgcgccg cgctcgggtg cgggcgcgcc cggctcgcac gccagttgct tacggaaggg
     5101 ctgttgctcg ctacggttgg cggtgcggcc gggattgctg tggccttagc cgcaatccac
     5161 tacttccgtg tggcggcacc aatagagatg ccaccgggtg ccgatgtgcg gatgagttgg
     5221 ccggtcctgg ccttcaccgc cgtggtttcc cttgcgaccg cgctgctgtt cggagcgctg
     5281 cctgcatggc gcgcctcgcg ggcagtcgag agtctgcagg cggggcggag cagcgcgcag
     5341 gccacgccgc atcgtgtgat gaagacgctg attgcggccg agatggcgct ttccctggtg
     5401 ctgctggcgg gctcgggctt gttgatgcag agcgtgctgc gcctgggctc ggagcaactc
     5461 ggattcgaaa gccgcggcct ggtgacggca cgcgtgaagc tgccggtgga ggattaccag
     5521 aagccgggcc gccgcgtgca attctacgat cgcgtactcg aagcgctggg caataaggcc
     5581 gcgctctcga cgggactgcc gccacatggg tggtccgggc ttgcgacgct gcacatccca
     5641 ggccgggagc cctccggatc gcatgatgcc ggctaccgga gcgtctcacc gcgctatttt
     5701 cgggtgatgg gcgaacggct actgcgcggt cgcgagttca acgatcgcga ccgtgcgtct
     5761 tcggagccgg tggtgatcgt aaacgaggcg ctcgcacgcc gctactttgc taacgctgat
     5821 cctattggac aacgcatcgc gttgaacgaa ccttcggaga cgaacccgtg gcgggtgatc
     5881 gtcggggtgg tggcggatga aaagggctgg ggcggcttcg accgcatggg ctgggcggtc
     5941 atgccggggg cgtttaagcc gctcgcccag gatgtaccgc gctcagcggc tcttgttgca
     6001 cgcggctcag cggcagatct tcggcgggcg gtgacaggaa tagatgaaac agtggcgatc
     6061 ggcgaagttg aggcgatgga cgctgggctt gatagagtgc tcgcgtaccc gcgattccgc
     6121 gcggcgcttc tcagcgcgtt ctccctgttt gccgtgctgc tggcggcaat cggactgtac
     6181 ggcatgctcg ggcagttcgt tgtacagcgc actccggaga tcggcattcg catggcgatg
     6241 ggcgcgcgtc ccgcagacgt gctccggctc gtagcgtgtc aggccggcgc tccgctgacg
     6301 gctggactcg ctttgggcct cgcgggcgca ttggcgctca gccggtcgct cgcgagcgtg
     6361 ttatatgggg tgcaggcagg cgatcccgcg acattcgcgg ccgtctcgct cgcattgatt
     6421 gccgcaggag caattgcagc gttctttccg gcacgacgcg cggctcgcgt cgacccgatg
     6481 accgccctgc gcaatgagtg aaaccgaacc aggaacggcg cgaggggccg tttatgcttt
     6541 ggaccgcggc ggcacgtaat cgggcgggaa cgccgcaccg gctccccaga agtattcttc
     6601 catctgcttc atcaggaatt cctgcgcatc cgggttcccg aggttgaggc ggtattcgtt
     6661 catgatcatc ttcatttgct caacccaaag ccgccacgct tccttggaga cgttttcgta
     6721 aatccgctgt cccagctcgt tgtcaaacgg cggctcatcc aagcccgcca tttcgcgctg
     6781 gaacttcacg cagaaaattt tacgcccgcc gggcttctca gcatccatcg gtggaggaaa
     6841 tccgccgcca catcccatcg ctagctccta tcgtttgttg atacaaattc aggatagcag
     6901 acaaggcttt ggagaccgtg gttccgttca gccggcaccg ttttccggcc gccgttcttc
     6961 gcgcgcatct ctccgggcac gtagcatctc caggtattcc gagagtttga acggagtgga
     7021 ggcacctttc tccatgtcgc gctggacggc cttctgatag cgctgaagaa ggttccgcct
     7081 ctgactgcct tgaagattgc aatcgccaat gtccaggagc aggtcgatct cccaccgccg
     7141 gaaactgttg cgcttcatat tgccacgcag taactcctgg atgagcttgt tgaactgctg
     7201 caggatagcg tcgctatccg gttcccgtcc aagcattcca agaatctcat cggcgatttc
     7261 acgctccggc attagataaa ctggaagaaa tgggctcgga tacgtcaggc cctcgcgatg
     7321 cgtctgttcg cccggcagcg gtggaaaggc tgttcgaatt ttcactgctt gggttgctta
     7381 cgagcggata ccttgccgtc gcgggttccg gcgctctgga cgtacccact ctcgcggcca
     7441 ccgggatcgc tatcgttctg cgcgctttga tggccgctgg cgttctccgg cttcgcatat
     7501 cggacactgc cgtaacggtt gccacgctct gctatatcgc gttctatccg ctcgactact
     7561 tttttctctc cagagagttt cttgcggcga cggtacacct ggtcttcttc ctgctggtag
     7621 tgaaggtgct gacggccagg accacgcgcg attatgtctt cgtcaaaatc gtggcttttc
     7681 tggagctgct ggcagcgtcg gttctatcga ccagcctcag ttttttcgtc ttcctggcct
     7741 ttttcctggt attcggcgtc gccacgttta ccagttcgga gatacgccgt tccacgggga
     7801 agcgcgtagc cacttcgcgc gccggtctgc gttcgattca catacgcctc accgcgctga
     7861 gcctggtgat cgcgatgggc atccttggca tgacggccgg cctctttttc ctgctgcccc
     7921 gaacggcgcg cgcggcgttc cagcatttcg cttcgcaaaa ataccggctg cccgggtttt
     7981 cgaatgaagt gacgctcggg gaaatcggcg agatcaagcg acagagcaca ccggtgatgc
     8041 acgtgcgcat cgagggcaac cgcagccagt tgtatctgaa gtggcgcggc gccgcgctgg
     8101 cggagttcga cggcaagcgc tggtacaaca gcaacaacgg cggcggtgag attgtgaaag
     8161 tcgagaaagg gatcgcgacg ctggttcaga acccgcagag atggcgtatc ggtccgcgca
     8221 tcagttataa cgtcatcctc aaatccattg cctccgatac cctgtttctg gcggggaccc
     8281 cggagttttt gcaggttcag gcgagaaccg tcattcgcac accaaccgac agctaccagt
     8341 tgggatacgg agcgggtgac ggaatgcgct atatcggaca cagctttctc ggcagcgggc
     8401 ctacaccaga acccgccgct cctctcaccc cggccgcgcg gaacctctat acttcgcttc
     8461 ctggccccga cgcgagaatt tcgcagtacg ccgcgaagat cgcttcgggg ctgcgcagcg
     8521 acggagaacg tgcccgcgcg attgaaaatg agctccggac gcagtacggc tacacaatgg
     8581 agttgccgga gaaagagctg gcggatccca tttccgactt tctcttccgc cgccgcaaag
     8641 ggcattgcga atacttcgct tcctccatgg ccgtaatact tcgcaccctg aagattcccg
     8701 cgcgggtcgt gacgggattt cagagcggcc tgtacaaccc catcagcggc tggtacgtca
     8761 tccgggcgtc ggacgcgcat agctgggtgg aggcctatct ccccggacag ggttggacga
     8821 cgttcgaccc tacgccgccg gaccccggcg cgggcgcgat ctccgcctgg accaggctga
     8881 tgctctattt cgacgccgcg gatattttct ggcaggaatg ggtgctgaac tacgac
//
LOCUS       DASTMK010000077        13363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_419635, whole genome shotgun sequence.
ACCESSION   DASTMK010000077 DASTMK010000000
VERSION     DASTMK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13363
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419635"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VFQ79_03085"
     CDS             <1..1281
                     /locus_tag="VFQ79_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0138662.1"
                     /translation="EPAREIPIEDTADVIVCGAGPAGVAAAISAARTGARTRLFDVHG
                     CLGGIWTAGLLSNIIDCLDKGGIMDEILAGLKKMGAQIDGPRYDTEAMKLLLDRMTAE
                     AGVKILLHTRVVAAAKDGNRVTTIVTENKSGRQAWRAKTFVDTTGDGDLAAFAGCKFD
                     LGHPQSGKMQPMTLMAILTGIDYRELHARRLMRGDGKSSYAKGVSPNESKVNIVAELR
                     RAGVEPSYTGPALFPIRNDLIAFMVNHEYDYFGLNAEHVTKATIHARAEVNQVVDALR
                     AHGGVWKNVRLVATGAQIGVREGRRIHGLYSLNKDDLMRGARFEDAVCRVNVGVDIHS
                     IDPSTNKKTTDAGVKTKPYDVPLRSLIARDVDGLVMAGRCISGDFFAHASYRMTGHAV
                     PMGEAAGRLAAIAARTDRPPREVPFLEVIRKESA"
     gene            complement(1259..1918)
                     /locus_tag="VFQ79_03090"
     CDS             complement(1259..1918)
                     /locus_tag="VFQ79_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEU0138663.1"
                     /translation="MPELPVAFFSERTGEAHAGIGFAVALAGGGKRELETIVSILRFF
                     GLTSSTQQDSSQADAIRKIARALDQIDQERARYIAAFAYILSRVAHADMNVSPEETAQ
                     MERLVMEKSGLPEDQAVMVVQIARHQNLLFGATEDFLVTREFREIASREERLALLDCL
                     FAVAAAEHHISLAEENEIRRISDQLLLEHRDYIAVKSRYRDHLAVLKPASKRATRSPS
                     G"
     gene            2237..2617
                     /gene="hisI"
                     /locus_tag="VFQ79_03095"
     CDS             2237..2617
                     /gene="hisI"
                     /locus_tag="VFQ79_03095"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEU0138664.1"
                     /translation="MDLNFSKLDGLITAVIQDHASGRVLMVGFMNEEAFRKTVETGFA
                     TFYSRSRRKLWLKGETSGHRLLVKGISTDCDSDALLIAVEPLGPGVCHNGYHSCFYRR
                     LEDGRWVETEPQVYQPEAVYGSEK"
     gene            2614..3483
                     /gene="hisG"
                     /locus_tag="VFQ79_03100"
     CDS             2614..3483
                     /gene="hisG"
                     /locus_tag="VFQ79_03100"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU0138665.1"
                     /translation="MILKLGIPKGSLETATVELFRRAGFSITVNGRSYFPAIDDPEIE
                     CMLIRAQEMARYVEDGILDAGLTGRDWVEENDAHVVTIADLIYAKQSFGKVRWVLAVP
                     DSSPFLSVKDLQGKVIATELVGATRRYLSRHGVTAKVEFSWGATEVKPPVLADAIVEV
                     TETGSSLRANKLRIIDTVLESNTQFIANTGAMQDEWKRRKLEDIRMLLEGAINALGKV
                     GLMLNVRKQDLSAVLGVLPALKNPTISHLSDEEWVAVNTILDESTVRNIIPRLKEAGA
                     QGIVEYPLNKIVM"
     gene            3485..4726
                     /gene="hisD"
                     /locus_tag="VFQ79_03105"
     CDS             3485..4726
                     /gene="hisD"
                     /locus_tag="VFQ79_03105"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU0138666.1"
                     /translation="MIRIVDSSQVSRILARKAARFAEAEAVVRPILEAVRKRGDKAVL
                     EYARKFDGFDRRSVLIPEKELSAARDRLSPSFRAAVRTASRNIRAFAKLQMPEARSTV
                     LDRGLRVGQIVRPLDTVAAYIPAGRYPLPSTLMMTAIPAQVAGVENICVATPRAVDEI
                     FGTAALLKITKVFQIGGAHAIAAFAYGTRTVPKADRIVGPGNIYVAAAKKLLAGEVGI
                     DFIAGPTEILIVAEEGTPAWIAADMLAQAEHDVEASAILLTPSRRLATAVAQEIETRL
                     ADLPTAAVARKSIDRNSAIILVRSTEEAIDIANRFAPEHLSIPNSKLLSRVRHAGSVF
                     VGPGSPEAAGDYASGPNHVLPTSGAARGRGGLSAADFVKVISVQELSLSALACLAPAV
                     TTLARAEGLEAHARSVEVRLD"
     gene            4719..5771
                     /gene="hisC"
                     /locus_tag="VFQ79_03110"
     CDS             4719..5771
                     /gene="hisC"
                     /locus_tag="VFQ79_03110"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0138667.1"
                     /translation="MIKLKPRDAVLKMAAYVPPTGGRAGKVRLDFNENTVGASPHVVS
                     FLKEQLTAGGLSVYPEYAEAKSEISAFFGVQPDELLFTNGTDEAIQVLVNTYVDDADE
                     VLLLRPSYAMYRFYAEVAGASIREVDYRHEDLSFPLKELLAQVQPRTKAILIANPNNP
                     TGTGIDLAGIIRILAAAPHAAVLIDEAYYEFCGITALPLIREYTNLFVSRTFSKVYGM
                     AAMRMGCLFSQPANTGFLHKAQSPYSVNTLATLGARAAIQDTQYVRNYVKQVIEARDI
                     LYKGFERMGIAYYRSQANFVLFRAGDRAVEIRDKLRAQDLLVRDRSYELPGCVRVTVG
                     TKEQVRKFLDALEEVW"
     gene            5765..6448
                     /locus_tag="VFQ79_03115"
     CDS             5765..6448
                     /locus_tag="VFQ79_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0138668.1"
                     /translation="MVKPDQMVVFDMDGVLVDVSESYRGAIVETVRHFTGKQISAEMV
                     QDYKNAGGWNNDWALSQKIAADLGVPVDYETVVEHFNKIFLGNGTDGLIARERWIPRP
                     GLLESLAERYRLAIFTGRLQCEAGITLRRFASELRFARIICSDDVANGKPAPDGLLQI
                     AGEFPGCCLWYVGDTVDDARSARAANVKFIGIAAPASPRREELAALLAQENAVAVLDD
                     INTLETVLV"
     gene            6445..7032
                     /gene="hisB"
                     /locus_tag="VFQ79_03120"
     CDS             6445..7032
                     /gene="hisB"
                     /locus_tag="VFQ79_03120"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEU0138669.1"
                     /translation="MRAATIERNTKETRIRGSLKVDGRGRYQVSTGIRFLDHMLELFA
                     KHGGFDLTLACDGDLDVDQHHTVEDAGIVLGQLFSKALGDRKGINRAGYFVLPMDETL
                     AVVALDLGGRPALVYKDLVKVRLVGDLQTELVEDFFGGFVNHAGANLHVKVLYGRSNH
                     HKIEAVFKCFARALRYACSKDARLRDQLPSTKGLL"
     gene            7029..7625
                     /gene="hisH"
                     /locus_tag="VFQ79_03125"
     CDS             7029..7625
                     /gene="hisH"
                     /locus_tag="VFQ79_03125"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU0138670.1"
                     /translation="MIAILDYGAGNLRSVQNTLEEIGASYELVRDASGLQRASKIILP
                     GVGHFGQMMRALDGLKVREALAGRIRVGVPFLGICLGLQALFSSSEEAPDVNGLNLFE
                     GTVRRFPAGVRIPHMGWNRLEPKGQPRLLRNLRAAPYVYFAHSYYVPEVEVTAATCTY
                     KVRYTALLEAGHIFGVQFHPEKSGPAGIEIVHNFVEFE"
     gene            7626..8390
                     /gene="hisF"
                     /locus_tag="VFQ79_03130"
     CDS             7626..8390
                     /gene="hisF"
                     /locus_tag="VFQ79_03130"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEU0138671.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGINFVNLRDAGDPVELAERYNDQGA
                     DELVFLDITASSDARDTMADVVARTARKVFIPLTVGGGIRTVADARKILMSGADKVSV
                     NTAAVRRPELIAELSQEFGSQAVVLAIDAKRRSECAWNVYTRGGRDDEGVDAVEWAQR
                     AESLGAGEILLTSMDTDGVQQGFDCDLTRTVSEATNIPVIASGGAGKPEDFLKVLTEG
                     CADAALAASIFHYGTYTVGDLKKYLESNNIPVRRLT"
     gene            8387..9121
                     /locus_tag="VFQ79_03135"
     CDS             8387..9121
                     /locus_tag="VFQ79_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEU0138672.1"
                     /translation="MIIPCIDLMDGKVVQLIQGREKALEGDAPLEMLSRFAQFPEIQV
                     IDLDAAIGRGSNDELVQLLASKAVTRVGGGVRTVERAKKLLEQGASKIIVGTAAFQAN
                     GIHHEFLQELVRAVGREPILIALDSKDGRIVVKGWRESIDVTAEDVLRDLEPFAGGFL
                     CTYVDKEGMMQGTDLDWFRRLRRATSSEITAAGGITTLEEIRALNDMGVHAALGMAIY
                     TGRLDLSELARLNRCSGAATPEHPLI"
     gene            9153..11672
                     /locus_tag="VFQ79_03140"
     CDS             9153..11672
                     /locus_tag="VFQ79_03140"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0138673.1"
                     /translation="MRLLFFIVLVFALLLIPVPGLQGQWELSGTAQIQQALAALTTTG
                     SVLMIAAHPDDENTALLAYFARGRNARTAYLSLTRGEGGQNLVGPEQGPELGVIRTQE
                     LLAARRIDGASQLFTRAIDFGFSKTAEETLAKWGHQEVLSDVVWNIRRFRPDVIILRF
                     SGTSRDGHGHHQCSAMLGKEAFFAAADPAKFPEQLKWVQPWKAKRVLFNVFSFTREQE
                     KEAAGLRTAIEIDTGEYNPVLGRSYSEIAGLSRSMHKSQGFGAAQRRGPVKNYLTLVA
                     GEQAAKDPFDGVDTSWGRIPGGAAAGELLTRAAQAFQPEHPERTVPLLLEARRMVRAI
                     QEPLAKYKLQEIDEAIALCAGLWLDAAADRYEAVPGSDLKIALTAINRSRAEISVEAM
                     SVEGQSAPGESVKLEYNQPHVRNIQFQVPAQQAYSQPYWLAKPVQGALYSVPDQKLIG
                     VPENPPALGARFRLRAEQEVIEITRPVHYRYVDSLRGEVIRPLAIVPPVAVQVSAVPL
                     VFPDAQPRATEVRVRANASNSSGRIEWTFPSGWRAEPAQADYRIGEPGESTVLRFTIT
                     PPAGDARAEAIAAAHTTDGRTVSQGGKTIDYPHIPPQALFPPSTLSLVRADIRMLSKR
                     IGYIEGAGDEVAEALAAVGYEVTLLTPQDLTAGDLGRFDAIVTGVRAYDVRPDLDANR
                     ARLLRYVEEGGTLVVQYNKLESERRRPSGFALAPYPMTIGWARVSVEDAPVAFPDPES
                     VLLAKPNRITTEDFNNWVQERGLYFATKWDPRYKTLFSSHDPGEEPLEGGTLYTPLGK
                     GAYVFTAYSWFRQLPAGVPGAFRIFSNLLSAGKTLYDQTHR"
     gene            11653..11781
                     /locus_tag="VFQ79_03145"
     CDS             11653..11781
                     /locus_tag="VFQ79_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138674.1"
                     /translation="MTRPTDEPPPFLGTWRRVYAAVLLWLAMLIFAFYLFTRAFNQ"
     gene            11778..>13363
                     /locus_tag="VFQ79_03150"
     CDS             11778..>13363
                     /locus_tag="VFQ79_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU0138675.1"
                     /translation="MRSLDWVVLFLSVVFIVFYGLWRSRGSNTVNKFLLAGKTMPWYA
                     MALSIMATQASAITFISGTAQAYVDGMRFVQMYFGLPLAMVILSATAVPLFHRSGVYT
                     AYEFLERRFDAKTRALVSAIFLLQRGLACGVALYAPAIVLTAILGIPDRATTLLMGSL
                     VLIYTVAGGIQAVTWTDFQQMLIMFAGVFISLGMAIALLPGDTSVMDAFALAGAAGRL
                     NPVVLSFDWQDEFNIWTGLLGGMFLALAYFGTDQSQVQRYLTGKSIEQSRLSLLFNAV
                     AKIPMQFLILLTGAMVFVFYSFEKPPIIFNPVELQRLESQPGHADLTRRYDAAYAERR
                     AAASRYVKTKDEAALTEYRAANVRMENVRKETGTTDTNYIFLSFVTRYLPVGVVGLIM
                     AAIFAAAMSTISAEVNSLATVSVVDIYRRHIKRDATDAHYLFASRAFTAFWCTYAIVT
                     AQLGAGPVSLIVMVNKIGSYFYGGMLGVFALAFFFPRVRGTAAFVGVLAGEAAILGAS
                     QLTQVSFLWYNVIGALVVIGT"
BASE COUNT         2651 a         3842 c         4143 g         2727 t
ORIGIN      
        1 gaacctgcgc gggaaatccc catcgaggat accgccgacg tgatcgtctg cggtgccggc
       61 cccgccggag ttgcagcagc catcagcgcg gcccgcaccg gagcgcgaac gcggcttttt
      121 gacgtgcacg ggtgcctggg cggcatctgg actgccggtc tgctttcgaa catcatcgat
      181 tgcctcgaca agggcggcat catggatgag attctggccg gtttgaaaaa aatgggtgcg
      241 cagatcgacg gaccgcgcta cgacaccgaa gcaatgaaat tgctgctgga ccgcatgacc
      301 gcggaagccg gtgtcaagat tctgctgcat acaagggttg tagcggcagc caaggacggg
      361 aaccgggtca ctacaatcgt tacggaaaac aagtcgggac ggcaggcgtg gcgggccaag
      421 accttcgtcg ataccaccgg tgacggcgat cttgctgcct tcgccggatg taagttcgat
      481 ctggggcatc cgcaatccgg caagatgcag ccaatgacgc tgatggccat cctgaccggt
      541 atcgactacc gggaactgca cgcgcgccgg ctgatgcggg gcgatggcaa gtccagctac
      601 gcaaaaggtg tctcgcccaa cgagtccaaa gtgaatattg tggcggaact gcgccgggcc
      661 ggagtggagc cttcctatac cgggccggct ctatttccca tccgcaatga cctgatcgcg
      721 ttcatggtca atcacgagta cgactacttc gggctgaacg cggagcatgt aaccaaggcg
      781 acaatccacg cgcgcgcgga agtcaaccag gtggtggatg cattgagagc gcatggcggc
      841 gtgtggaaaa acgtgcggct ggtagcaacc ggggcgcaga tcggcgttcg ggagggacgc
      901 cgcattcatg gcctgtattc gttgaacaaa gacgatctga tgcggggcgc gcgttttgaa
      961 gacgcggtgt gccgggtgaa tgtcggagtg gatattcatt ccatcgatcc gtccactaat
     1021 aagaaaacga ccgatgccgg cgtgaagacg aaaccctacg acgtgccgtt gcggtctctg
     1081 attgccaggg atgtggacgg actcgtgatg gccggacgct gcatcagcgg cgatttcttc
     1141 gcccacgcca gctatcgcat gaccgggcat gccgttccaa tgggagaagc cgcgggccgt
     1201 ctggccgcca tcgccgcgcg cacggaccgg ccaccgcgtg aggtgccctt cctggaagtt
     1261 atccggaagg agagcgcgta gcccgcttgc tggctggttt caatactgcg aggtgatcgc
     1321 gataacggga ttttacggcg atataatcgc gatgctccag cagaagctga tcggagatgc
     1381 ggcggatctc attctcttcg gccagcgaaa tatggtgttc ggccgcagcg acggcaaaca
     1441 ggcagtcgag cagcgccagg cgttcttcgc gcgacgctat ttctctgaac tcgcgggtta
     1501 ccagaaaatc ctcggtcgca ccgaaaagca ggttctggtg cctggcgatc tggaccacca
     1561 taacggcctg gtcctccggc aagccactct tctccattac caggcgttcc atttgggcgg
     1621 tttcttccgg gctgacgttc atgtctgcgt gcgccactcg gctgagtatg taggcaaaag
     1681 cggcgatgta cctcgcccgt tcctgatcga tctgatccag agcccgggcg atcttccgga
     1741 tggcgtcagc ctgcgacgaa tcctgctgtg tgctacttgt taatccgaaa aaccttagga
     1801 tggacacgat agtttctaac tctctcttac ctccaccggc taacgcgacc gcaaagccga
     1861 tgcctgcgtg cgcctccccc gttctttccg aaaaaaacgc gactggcagt tccggcatcg
     1921 aaacggcacg taggaaatca ctcgcataac gtgagaaagg aaatcgcggg tgcgcgagcg
     1981 gcgaatgtcg ctggaaccgc attttggaca cgtcaccata ccggcatccc tctccttttc
     2041 agggtaacag ttcagaattg cacccggtta tttctctgat ctggtatact gatgggttca
     2101 tgtactactg ccggtacgcc cgacatcccc accatcgcca tcatacggtt gtctcgctat
     2161 cgggctgaac ggagatctaa aaatcgagtt gggagcccgc cgcaattcag gcgggctttt
     2221 ttgttagctg gttcacatgg atctgaactt ctcaaaattg gatggactga tcaccgccgt
     2281 catacaagac catgcctccg ggcgcgtcct gatggtggga tttatgaatg aagaggcttt
     2341 tcgtaagacc gtcgagaccg gctttgccac gttctacagc cgctcccggc gcaagctgtg
     2401 gttgaagggc gagacctccg gacaccggct gttggtgaaa gggatctcga cggactgcga
     2461 ttccgacgcg ttgctgatcg cagtagaacc cctgggaccc ggcgtctgtc acaacggata
     2521 tcacagttgc ttttatcgca ggctggaaga cggccgatgg gtggagaccg aaccgcaggt
     2581 ataccagccc gaggccgtat acgggagcga gaagtgatcc tgaagctcgg cattccaaaa
     2641 ggcagcctcg aaaccgcgac agtggaactc ttccgccggg ccggtttcag tatcacggtg
     2701 aacggacgtt cgtatttccc ggctatcgac gacccggaaa ttgaatgcat gttgatacgc
     2761 gcgcaggaaa tggcgcggta tgtggaggac ggcatactcg atgccggact taccgggcgt
     2821 gactgggtag aggagaacga cgcccacgta gttaccatag ccgacctcat ttacgccaaa
     2881 cagagcttcg gaaaagtgcg ttgggtgttg gccgttcccg attcgtctcc ctttctttcg
     2941 gtgaaggacc tgcaaggtaa agtgattgcc acagagttag taggcgccac ccggcgctat
     3001 ctttccaggc acggtgtaac agccaaggtg gaattcagtt ggggagctac ggaagtgaaa
     3061 ccacccgtcc tggcagacgc catcgtggag gtgaccgaga ccggctcgtc gctgcgggcc
     3121 aacaaactcc gcatcatcga cacggtgctg gaatcgaaca cacagttcat cgccaatacc
     3181 ggcgcgatgc aggatgaatg gaagcggcgc aagctggagg atatcaggat gcttctcgag
     3241 ggtgccatca acgcgctcgg gaaagtcggc ctgatgttga atgttcgcaa gcaggacctg
     3301 agcgcggttt taggtgttct gcccgccctg aaaaatccca ccatctcaca cctgagcgat
     3361 gaagagtggg tggccgtcaa taccattctc gacgaatcca cagtccgcaa tatcatcccg
     3421 cggctgaagg aagccggcgc gcaaggcatt gtcgagtatc cactcaataa gatcgtgatg
     3481 tgacatgatc cgcattgttg attcgagtca ggtaagcaga attctcgcca gaaaggccgc
     3541 gcgattcgcc gaggccgaag cagtggtgcg tcctattctg gaggcagtcc gcaaacgcgg
     3601 agacaaggcg gttctcgaat acgcccggaa gtttgatggt ttcgaccggc gcagcgtgtt
     3661 aattccggag aaggagttga gcgcggcacg cgatcgcctg agcccgagtt tccgggcagc
     3721 cgtcagaacc gcttcccgga acatccgggc attcgcgaag ctgcaaatgc cggaagcccg
     3781 ctccactgtg cttgatcgcg gcctgcgcgt aggacaaatt gtgcgcccgc tggacaccgt
     3841 tgcagcctat attcccgctg ggcgctaccc gctgccctcc accctgatga tgacggccat
     3901 tcccgcccag gtggcaggcg tggagaacat ctgcgtcgca acccctcgcg cggtggacga
     3961 gattttcggc acggctgctc tgttgaagat cacgaaagta tttcagatcg gcggagccca
     4021 tgcgattgcc gcgttcgctt acggcactcg tacggtgccc aaggcggatc gtattgtcgg
     4081 gcctggtaat atctacgtcg cggcggcaaa gaagctgctc gcgggcgaag tgggaatcga
     4141 tttcatcgcg ggtccgacgg agatattgat tgtcgccgaa gaggggacgc cggcgtggat
     4201 cgcggcggac atgcttgcac aggcggagca tgatgtcgaa gcttccgcta ttctcctgac
     4261 cccttcgcga cgtctcgcca ccgccgtagc gcaggagatc gaaacccggc tcgcggacct
     4321 gccgaccgcc gccgtcgctc gtaagtcgat cgaccggaac agcgcgatca tactggtccg
     4381 ctccaccgaa gaagcgattg acatcgcgaa ccggttcgca ccggagcatc tgagcattcc
     4441 aaattcaaaa ctgctcagcc gtgtgcggca tgccggtagt gtcttcgtgg gaccgggcag
     4501 tccggaagcg gccggcgact acgcctcggg accgaatcat gtgttgccca cttcgggcgc
     4561 cgcaagaggg cgcggcggcc tttccgccgc cgattttgtg aaagtgattt cggttcagga
     4621 gctttcgttg agcgcactgg cgtgccttgc tcccgcagtc accacgctgg caagggccga
     4681 gggtctggag gcgcatgcgc gctcggtgga ggtgcggctt gattaagttg aaacctcgcg
     4741 acgccgtgtt gaagatggcg gcctatgtgc ccccgacagg cggccgtgcc ggaaaagtga
     4801 gactcgattt caacgagaac accgtgggcg cctcgccgca tgtcgtaagc ttcctcaagg
     4861 agcaactcac cgccggcggg ttgtccgtct atcccgaata cgcggaagcg aagtccgaga
     4921 tctccgcctt cttcggtgtt cagccggacg aactgctgtt caccaacggc acagacgagg
     4981 cgatccaggt gctggtgaac acgtacgtgg acgatgcgga cgaggtgctg ctcctccggc
     5041 cttcctacgc tatgtaccgc ttctatgccg aggtcgccgg cgcgtcgata cgggaagtcg
     5101 attaccgtca cgaagacctg agttttcctt tgaaggaact gctggctcag gtacagccgc
     5161 gtacgaaggc catcctcatt gccaatccga acaatccgac cggcacggga atcgatctcg
     5221 ccggtatcat ccggattctg gctgccgcgc cgcatgcggc ggtgctgatc gatgaagctt
     5281 actacgagtt ttgcggtatt accgcgttgc cgctgatacg cgaatacacg aacctgtttg
     5341 tgagccgtac gttttcgaag gtgtacggga tggcggcgat gcgaatgggg tgtctgtttt
     5401 cgcagcctgc taataccggc ttcctgcaca aggcgcagtc gccatacagc gtcaacacgc
     5461 tggctacgct cggcgcgcgc gccgcgattc aggacacaca gtacgttcga aattatgtga
     5521 agcaggtgat cgaagcccgg gacatcctct acaagggatt cgaacggatg ggaatagcat
     5581 actaccgcag ccaggcgaat ttcgtgctgt ttcgcgcggg agaccgcgcc gtcgagatcc
     5641 gcgacaagct gcgggctcag gatttgctcg tgcgggaccg cagttatgag ctgcccggct
     5701 gtgtccgcgt gacggtgggt acaaaagagc aggtccggaa gtttttggac gccctcgagg
     5761 aggtatggtg aagccggacc agatggtcgt tttcgatatg gatggcgtac tggtggacgt
     5821 ttccgagtcc taccgcgggg ccatcgttga aaccgtgcgc cattttacgg gaaaacagat
     5881 cagcgccgaa atggtgcagg actacaagaa cgcgggcgga tggaataacg actgggcgct
     5941 ttcgcaaaag atcgcggcgg accttggcgt cccggtcgac tacgaaacgg tggtcgagca
     6001 ctttaacaag atctttcttg gcaacggcac ggacggactc atcgcgcgcg agcgctggat
     6061 cccgcgtcct ggcctgctcg aaagcctggc ggaacggtac cggctggcaa ttttcaccgg
     6121 acggctccag tgcgaggccg gcattactct tcgcagattc gcctccgagt tgcgattcgc
     6181 ccgcatcatc tgctcggatg acgtagccaa cggcaagccc gcgccggatg gccttttaca
     6241 gattgccgga gagtttccag gttgctgcct gtggtatgtc ggcgacacgg tggacgatgc
     6301 gcgctcggcg cgagccgcaa acgtgaagtt catcggcatc gccgctccgg cgagccccag
     6361 gcgggaagaa ctggctgcgt tgctggccca ggaaaatgcg gtggcggtgc tcgacgatat
     6421 caatacgctt gaaacggtgc tggtatgaga gccgctacga tcgagcggaa cacgaaagag
     6481 acgcgcatcc gcggctccct caaggttgac ggccgcgggc gttaccaggt gtcgaccggt
     6541 atccggtttc tcgatcacat gctggaactg tttgccaagc acggcgggtt cgacctgact
     6601 ctcgcttgtg acggcgatct ggacgtcgac cagcatcaca ccgtggagga tgccggcatc
     6661 gttctcgggc aattgttttc gaaagcgctc ggcgaccgga agggtattaa cagggcgggg
     6721 tatttcgtac ttcccatgga tgagacgctg gcagtggtcg cgctggacct gggcgggcgc
     6781 cccgcgctgg tgtacaagga cctggtgaag gtccggttgg tcggggatct gcaaacggag
     6841 ctggtggaag acttcttcgg aggcttcgtc aaccatgccg gcgccaactt gcatgtgaaa
     6901 gtgctgtacg gccggtcgaa tcatcacaag atcgaagccg tcttcaagtg ttttgcacgc
     6961 gctttgcggt acgcctgttc caaggatgcg cggcttcggg atcaactccc atccacgaag
     7021 gggctgctgt gatcgcgatt ctcgattacg gcgcgggaaa cctgagatcc gtacagaaca
     7081 cgctcgagga aatcggagcg tcctacgaac tcgtcagaga tgcttccgga ttgcagcggg
     7141 cgtcgaagat catcctgccc ggcgtgggcc acttcggcca gatgatgcgt gcgctggacg
     7201 gcctgaaagt gcgggaagcg cttgccggcc gtattcgcgt gggcgtgccg ttcctgggca
     7261 tctgtcttgg tcttcaggcg ctgttttcgt cgagcgagga agctccggat gtaaacggcc
     7321 tcaatctctt tgagggaacg gtccgccggt ttcccgccgg ggttcgtatt ccccatatgg
     7381 gttggaaccg cctggaaccg aagggccagc cgcgtcttct gcgcaatctc cgggccgcac
     7441 cgtacgttta cttcgcacat agttactatg tgccggaagt ggaagttact gcggctacgt
     7501 gtacttacaa agtgcgttat acggcgctgc tcgaagccgg acatatcttc ggcgttcagt
     7561 ttcatcccga gaaatccggg ccggccggaa ttgaaatcgt tcataacttc gtggagttcg
     7621 aatagatgtt ggcgaaacgc ataattcctt gtttggatgt gaccggcggg cgcgtggtga
     7681 agggcatcaa cttcgtgaac ctgcgtgatg ccggagaccc ggtcgaacta gctgaacgct
     7741 ataacgatca aggcgccgac gaactggtat ttctcgacat caccgcttcg agcgacgcgc
     7801 gggataccat ggccgacgtg gtggcgcgga cagcgcgaaa agttttcatt cccctgaccg
     7861 tcggcggcgg cattcgaacg gtggcggacg cccggaaaat tctgatgtcc ggtgcggaca
     7921 aagtgagcgt gaacactgcc gcggtccggc gtcctgaact gatcgccgag ctgagccagg
     7981 aattcggatc gcaagccgtc gtcctggcca tcgacgccaa gcggcggtcc gaatgcgcct
     8041 ggaacgtcta cacacggggc ggccgggacg acgagggggt ggacgccgtc gagtgggcac
     8101 agcgcgcgga gagcctgggt gcgggggaga tcctgctaac gtccatggac accgatggtg
     8161 tccagcaggg attcgattgt gatcttactc gcacggtttc ggaggctact aacatcccgg
     8221 ttattgcttc aggaggcgcc ggcaagccgg aagatttcct caaagtgctg acggaaggat
     8281 gcgccgacgc cgcactggcc gcctccatct ttcactatgg aacttatacc gttggcgatc
     8341 tgaaaaagta tctcgagtcc aataatatac cggtcaggcg gctaacttga ttattccttg
     8401 tatcgatctg atggatggta aggtcgtcca actgattcag ggccgggaaa aggcgctaga
     8461 gggagacgct ccgctggaga tgctctcccg gtttgcacag tttccggaaa ttcaggtgat
     8521 cgatctggat gccgctatcg gcaggggttc caatgacgaa ctcgtgcagt tgctggcttc
     8581 aaaagcggta acgcgtgtcg gcggcggagt gagaaccgtc gagcgggcga agaagcttct
     8641 ggagcagggc gcgagcaaga taattgtcgg gaccgccgca tttcaagcaa acggcataca
     8701 ccacgagttc ctccaggagt tggtgcgggc cgtcggacgg gagccaatcc tcatcgctct
     8761 cgattccaaa gacggccgta tcgtcgtgaa aggttggagg gaatccatcg atgtcaccgc
     8821 ggaggacgtg ttgcgggacc tggaaccctt cgccggcgga ttcctttgca cgtatgtcga
     8881 caaggaaggg atgatgcagg gcacggatct cgactggttc cgtcgtcttc gccgggctac
     8941 ttcttcagag atcacggccg ccggtggcat cacgaccctg gaggagatcc gcgcattgaa
     9001 cgatatgggt gtacacgcgg ccctggggat ggcaatctac actgggcgcc tggacctgtc
     9061 ggagctggcc cgcctcaatc gatgttcggg cgcggcaacg cctgaacatc ccctaatata
     9121 aaaagacggc tggcgcgttc cactaacagc acatgcgctt actatttttc attgtcctgg
     9181 tcttcgctct gctgcttatt ccggtacccg gtttgcaagg gcaatgggaa ctctccggaa
     9241 cagcgcaaat ccaacaggcc ctcgctgcgc tgacgaccac cggcagcgtg ttgatgattg
     9301 cggcgcaccc ggacgacgag aataccgccc tgcttgccta tttcgccagg ggccgcaacg
     9361 cgcggacggc ttatctgtcg ctgacgcgag gtgaaggcgg ccagaatctg gtcggtcccg
     9421 aacaggggcc cgagttgggt gtcatacgca cacaggaact gctggcggcg cggcgcatcg
     9481 acggcgcaag ccagttgttt acgcgcgcca tcgatttcgg attttcgaaa acggccgagg
     9541 agacgcttgc gaagtgggga catcaggagg tgctctccga cgtcgtatgg aacattcggc
     9601 gcttccggcc cgatgtcatt attctgcgct tttccggtac ttcgcgcgat ggacatgggc
     9661 atcatcaatg ctcggccatg ctgggcaaag aggcgttttt cgccgcggcc gatcccgcga
     9721 aattcccgga acagctcaaa tgggttcagc cgtggaaagc gaaacgtgtt ctattcaacg
     9781 tgttttcttt tacgcgggag caggaaaaag aagcagccgg gctccgcacg gccatcgaga
     9841 ttgataccgg cgaatacaat cccgtgctgg gccgctccta tagcgagatc gccgggttga
     9901 gccggagcat gcacaagagc cagggcttcg gcgccgccca aaggcgcggg ccggtgaaga
     9961 actacctgac actggttgcg ggcgagcagg cagccaagga tccgtttgac ggcgtagaca
    10021 cgagctgggg ccggattccg ggaggcgccg cggccggcga attgcttacc cgggccgcac
    10081 aggcgtttca accggaacac cctgagcgca ccgtgccgct ccttctcgaa gcacggcgga
    10141 tggtgcgcgc gatacaggag cctctcgcga aatataagct ccaggaaatc gacgaagcca
    10201 tcgcgctctg tgccggtttg tggctggatg cggccgccga ccgttacgaa gcagtgccgg
    10261 gttccgatct gaaaatcgct ctgacagcga tcaatcggtc ccgcgcggag atttcggtcg
    10321 aggcgatgtc ggtcgaaggc caatccgcgc cgggtgagag cgtaaagctt gaatacaacc
    10381 agccgcacgt gcgcaatatt caattccagg tgcccgcgca gcaggcctat tcgcagccgt
    10441 actggctggc caaacccgtc cagggcgcgc tctattccgt cccggatcag aagctcatcg
    10501 gagttccgga gaaccctccg gcgctcggtg cccgcttccg gctgcgcgcc gagcaggaag
    10561 tgatcgagat tactcgcccg gtccactatc gatatgtgga ttctctccgt ggcgaagtaa
    10621 ttcggccttt ggctattgtc ccgccggttg ccgtgcaggt gtctgcggtt ccgctggtgt
    10681 ttcccgatgc acagccacgc gctaccgagg tccgggtgag ggccaatgcc tcgaactcat
    10741 cgggccggat cgaatggacg tttccgtccg ggtggcgggc agagccggca caggcggatt
    10801 acaggatcgg tgagccgggc gaatccaccg tcttgcgatt cacgattacc cctccggccg
    10861 gcgatgctcg cgcggaagcg attgctgcgg ctcacaccac ggatggtcgc actgtatctc
    10921 agggtgggaa gacgatcgac tatccgcata tcccgccgca ggcgctgttt cctccgtcga
    10981 cgttgtccct ggtgcgcgcg gatatccgca tgctgtcgaa acgcatcggc tacatcgaag
    11041 gagcggggga tgaagtggcc gaggcgctcg ccgcggtggg ctatgaagtg acgctgctta
    11101 cgccccagga cctgacagcc ggagatctgg gccgttttga cgccatcgtt accggggtgc
    11161 gtgcttatga tgtgcgtccc gacctcgatg ccaaccgcgc ccggcttctc cgttacgtcg
    11221 aagagggggg cacgctggtg gtgcaatata acaagctgga gagcgaacga aggcggccca
    11281 gtggattcgc tctggcacca tatcccatga cgatcggctg ggcgcgggtg agtgtggaag
    11341 acgcaccggt cgcatttccg gaccctgaaa gcgtgctgct cgccaaaccc aaccggatca
    11401 ccacggaaga cttcaacaac tgggttcagg agcgcggatt gtacttcgcg acgaaatggg
    11461 accctcgtta taaaacgctg ttcagttcac acgatccggg ggaagaacct ctcgaaggtg
    11521 gtacactcta cacgccactc ggcaaggggg catacgtgtt caccgcgtat tcctggttcc
    11581 ggcagcttcc cgccggtgtg ccgggagctt ttcgcatttt ttcgaacctg ctgagcgccg
    11641 gaaaaacatt atatgaccag acccacagat gagccgccgc ctttcctcgg cacctggcgc
    11701 cgcgtgtacg cagccgtcct gctgtggctt gccatgttga tcttcgcgtt ctatctgttc
    11761 actcgcgcgt tcaaccaatg agatcgctcg actgggttgt tctctttctg tcggtagtat
    11821 tcatcgtttt ctacgggttg tggcgcagcc gcggctcgaa caccgtcaac aagttcctgc
    11881 tggccgggaa gacgatgccc tggtacgcga tggcgctctc cattatggcc acgcaggcga
    11941 gcgccatcac gtttatctcg ggcaccgcgc aggcgtacgt cgacggcatg cgatttgtgc
    12001 agatgtattt cggcctgccg ctggcaatgg tgatcctttc ggcgaccgct gtccctctgt
    12061 tccatcggtc gggcgtctat accgcctacg agttcctcga acggcgcttc gatgcgaaga
    12121 cccgcgctct tgtcagcgcg atcttcctgc tacagcgcgg acttgcctgc ggcgtggcgc
    12181 tgtatgcgcc agcaatcgtg ttgacggcca ttctcggcat tccggaccgg gcgacgacac
    12241 tcctgatggg aagcctcgtg ctgatttaca cggtcgcggg tggtattcag gccgttacgt
    12301 ggacggactt tcagcaaatg ctcatcatgt tcgccggtgt tttcatctcg ctcggcatgg
    12361 cgattgcact gctccccggc gatacctctg taatggatgc cttcgcgctc gcaggcgcgg
    12421 ccggacggtt gaaccccgtg gttttaagct tcgactggca ggatgaattc aacatctgga
    12481 ccgggctgct gggaggcatg ttcctcgcac tggcctactt cggtaccgat caatcgcagg
    12541 tgcagcgata tctcaccggc aaatccatcg aacagagccg cctcagcctt ctgttcaacg
    12601 cggttgccaa gattccgatg cagtttctta ttctgctcac cggtgcgatg gtgtttgtct
    12661 tctactcgtt cgagaagccc cccatcattt tcaatccggt cgagttgcaa cggctcgaat
    12721 cgcaacccgg gcacgcggac ctcacccgcc gttacgatgc cgcctacgcc gaacgccgcg
    12781 ccgcggcgag ccggtacgta aaaacgaagg acgaagccgc gctcaccgag tatcgcgccg
    12841 ccaacgtccg gatggagaac gtcaggaaag agaccgggac gaccgatacg aactacatct
    12901 tcctcagttt cgtcacccgg taccttccgg tgggcgtggt gggtctcatt atggcggcga
    12961 tcttcgcggc agccatgtcg acaatctccg ccgaagtgaa ctcgctcgcg acggtcagtg
    13021 tggtcgatat ctaccgcagg cacataaaac gagatgccac cgacgcgcat tacctgtttg
    13081 cttcccgcgc atttaccgcg ttctggtgca cctacgccat cgttacggct caactcggcg
    13141 cggggccggt ctcgctgatc gtcatggtga acaagatcgg atcgtacttc tacggcggca
    13201 tgctcggggt gttcgcgctc gcattctttt tcccgcgggt gcgcggaacg gcggcattcg
    13261 tcggggttct ggcaggcgaa gcagccatcc tcggcgcgag ccaacttacc caggtctcat
    13321 tcctctggta caacgtgatt ggcgccctgg tggtgatcgg aac
//
LOCUS       DASTMK010000078         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_580167, whole genome shotgun sequence.
ACCESSION   DASTMK010000078 DASTMK010000000
VERSION     DASTMK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580167"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /gene="secD"
                     /locus_tag="VFQ79_03155"
     CDS             complement(<1..1455)
                     /gene="secD"
                     /locus_tag="VFQ79_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU0138676.1"
                     /translation="MPKKLRWKWLAIVAVVALCVTGVTGLPTSFEELAKNWRENIRLG
                     LDLKGGSHIVLQIQVQDAFKAEADRLIEQVKDLLSKNNIQYSSIDRNDPDSVETAATI
                     QVSIKGVPADRAADVRRLINDTAGQQWVLASEGPTGYRLNLRPEAAVNLRNDTLGQTM
                     NTLEKRVNGLGVAEASIQRRGGTEGEAEIVVQLPGVDDPARVKSILQTSALLELAEVI
                     GGPYPSREEALSQNGGILPLNSRLVPESLRGGSRAGWYILARSAVVTGRDLRNAQAQQ
                     NATNRTWETSFVLTQDAARRFERFTGAHIGDRLAIVLDNAVLSAPTIEAKISDNGVIT
                     NLGSQEDASDLALNLRSGSLPAGVRIGEERTVGASLGADSIRRGVTAGLVGLALVIAS
                     MIGYYRGAGWNAVLALLLNTIMTVAALSYIDATWTLPGIAGLVLSIGMAVDSNVLIFE
                     RIKEEMRAGKAVPAAIAAGFDRALVTIIDTHVTTV"
     gene            1623..1853
                     /locus_tag="VFQ79_03160"
     CDS             1623..1853
                     /locus_tag="VFQ79_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138677.1"
                     /translation="MQSRHADSRERQRGRCLKRRPEIPGAVVQIQKRLFTLASVRCTE
                     GRMGGAEARSCIIRLIRNPGERDGNQTCQQAF"
     gene            2228..>2675
                     /locus_tag="VFQ79_03165"
     CDS             2228..>2675
                     /locus_tag="VFQ79_03165"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0138678.1"
                     /translation="MSTFHVVRVLSLSVLLAASDPLYTTRAQTPSGQIFGVVTDPSAT
                     AVAGASITVQNETTNQQRVLTSGTAGDYVAGQLAPGRYRLTVRHPGFKAAVVSNLELQ
                     ALQNLRSDVQLSLGELAESVTVVSENIQVDTRGSSAGLLVDDKRVRE"
BASE COUNT          546 a          820 c          751 g          558 t
ORIGIN      
        1 aacggtggtc acgtgcgtat cgatgatggt cactaaagcg cggtcgaagc ccgccgcgat
       61 cgccgcgggc acggccttgc cggcgcgcat ctcctcctta atgcgctcga agatcagcac
      121 attggaatcg accgccatgc cgatagacaa gaccaggccc gcgattccgg gcaacgtcca
      181 ggttgcatcg atgtagctca gcgcggcgac ggtcatgatg gtgttcagca atagcgccaa
      241 cacagcgttc cagcccgcgc cacggtagta tccgatcatc gatgcgatca ccagcgccag
      301 gccgaccagt ccggccgtaa cgcctcgccg gatcgaatcc gcacccagcg aagcacccac
      361 ggttcgttct tccccgatcc tgacgccagc cggtagagac ccggatcgca ggttaagggc
      421 gagatccgaa gcatcctcct gcgagcccag attcgtgatc acaccgttgt cactgatttt
      481 ggcctcgatc gtcggtgcgc tcagaaccgc gttgtccagc acgatggcga gccggtcgcc
      541 aatgtgagct cccgtgaatc gctcgaaccg gcgcgcggcg tcctgagtca gaacgaaact
      601 ggtttcccag gttcgattgg tggcgttctg ctgcgcctgc gcatttcgaa ggtcgcggcc
      661 cgtaactaca gcagaacgag ccagaatata ccatcccgct ctcgagcctc ctcgcaaaga
      721 ctccggaacc aggcggctgt tcaaaggcaa gatgccgccg ttctggctga gcgcctcctc
      781 tcttgacgga tacggaccgc caatcacttc ggccagttcc agcagcgccg aagtctggag
      841 gatcgatttg acacgcgccg ggtcgtcgac acccggcaac tgaaccacaa tttccgcttc
      901 accttcggtt ccgcctcgcc gttgaatact cgcctcggca acacccagac cgttgactcg
      961 cttttcaaga gtgttcatgg tctggcccag agtgtcgttt cgcaaattaa ccgctgcttc
     1021 cgggcggaga ttcagacggt agccggtagg gccctcagag gccaggaccc actgctggcc
     1081 cgccgtatcg ttaatcaatc gccgaacatc tgctgctctg tcggcaggca cacctttgat
     1141 gctcacctgg atcgtagcgg cagtctcgac cgagtcgggg tcgttgcgat cgatggagct
     1201 gtactggata ttgtttttcg agagcaagtc tttcacctgc tcgatgaggc gatcggcctc
     1261 ggccttgaag gcatcctgca cctggatttg caggacgata tggctgccgc ctttcaagtc
     1321 cagcccaagc cggatgttct ctctccaatt cttagccagt tcctcgaatg aggtcggcag
     1381 gccggttacc ccagtcacac atagcgccac gacggcgaca attgcaagcc acttccaacg
     1441 cagtttcttt ggcatattcc ttctcccaat tcagaaattc aaattcccac ggcgcaagtc
     1501 cgcagggcac actaccgctc gttgtggagt caggcaatgt ggggaggggc ccggccaagc
     1561 gcagagcatg ttgcgccgga ttgccgaggt ccggaatcgc tgaagaaaat gatgacaccg
     1621 gcgtgcaatc gaggcacgcc gattcgcgcg aacgccaacg cggcagatgc ctgaaacgac
     1681 gtccggagat tcccggcgca gtcgtgcaga ttcagaagcg cttgttcacg ctggcgagcg
     1741 tccgttgcac tgaagggcgt atgggcggcg ctgaggcgcg gtcctgcatc atccggttga
     1801 tacgcaaccc cggtgagcgg gatggcaatc agacatgcca gcaggcattc tgaccgcagg
     1861 acttgacgca gtagtttcag cgcatcaccc cacgcgagag gttggaagcg cgagcgctcg
     1921 acggtccgtt tacggacgcc ggtacccgtt tctgtgattc gcgcttcatt ctccctcttc
     1981 tatgctactc ggaacggcgg gggaaaatca tcggaatagc gaaagtgcac tgcggatgca
     2041 cctgaatctc taaaagccat caactttccc cttgacattc cctctataac accctattct
     2101 ctatcaataa tatatactat tcaaaaaccg ctccccggct gttgttgtcg ctaattctcg
     2161 gtgggcagct aaatcactaa aaacaaagct cgattaaatt gcgcaccttg gtgcgaaagg
     2221 gggaattgtg tccacatttc atgtcgtacg tgtcttgtct ttgtctgtgc tgctggcagc
     2281 gtccgatccg ctatacacca cgcgagccca gactccgtcc ggtcagattt ttggggttgt
     2341 caccgatcca agcgccactg ccgtggccgg cgcttccatt acggtgcaga acgaaaccac
     2401 caatcagcaa cgggtcctca cttcgggtac cgcgggtgac tatgttgcgg gccaattggc
     2461 gcccggccgc taccgcctga cggtgcgtca tccgggattc aaggccgccg tggtctcgaa
     2521 cctggagctc caggcattgc agaatctgcg gtccgacgtg cagctttcac ttggtgaatt
     2581 ggcagagagc gtcaccgtcg tttcggagaa catccaggta gacactcgcg ggagcagcgc
     2641 gggcctgttg gtagacgaca agcgtgttcg tgagc
//
LOCUS       DASTMK010000079        17041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_419663, whole genome shotgun sequence.
ACCESSION   DASTMK010000079 DASTMK010000000
VERSION     DASTMK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17041
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419663"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..3569
                     /locus_tag="VFQ79_03170"
     CDS             324..3569
                     /locus_tag="VFQ79_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0138679.1"
                     /translation="MIARRVLILFVTAAVCVPVVSTGQTTGTISGYITDPAGAVVQTQ
                     VTAVLVQQNVSRTVESNTEGFYIFNALPPGTYNLSAQPAGFQRFVRSGVTLTVSQNVR
                     ADIRLQLGAVSETVEVSGEAPLVDTTSGTISGLVDDRRVVDLPLNGRNIIALARTVPG
                     VQNVKAPQSLNNARDGALMNVNGSASTMNVFTLNGANFINPSRNTGLNYPPPDALQEF
                     RILTNSFSAEYGRNAGSQVNVVSKAGTNAFHGALWEFLRNDNLNARNFFAARRPARVQ
                     NQFGGAAGGPVVRNKVFVFGTYQGLRDRPEAVSAQAIVPSTAQRSGDFSASSKVLRNP
                     IDPLTGAPFTDPAGLPCVQSNIIRPGCISPAISKHFLPLVPESPTGTVTTLSPSPINA
                     DLFMVRTDWLQSSKNTLFTNYFRDHNLRDRPTLAAGNIPGFQSSKDVTDTHLAVFSDT
                     YTVSPTLLNEFKVSFLRSTSESGASPTIEPRDLGINMFKYPAGGALSLSVGSLFSFGS
                     AGKVVFTSNNWQFRDTVSWIKGRHNVKFGGELLRMHFRQIFLGPPGFSFNGSRSGDEI
                     ADFLLGAFRTLSHGFGVRENDDFMTAPNFFFQDEFKIHPRFTLTYGVRYEPFLPWVDR
                     YDRLVSIEGVFSGQRSERFPDAPPGIVYPGDPGVSRGIVHNDLNNFAPRLAFAWDLFG
                     DGKTSVRGAYGVFYDHIKADAVAQENAPWAGSSQIFDGRVDDPYGSLGLTPPPVVPGG
                     EDFARFPLPIRYRGISDTLRTAYVQSWNVTIQRQLTGSVMLETSYVGKAGIKIEGARN
                     FNPAAFKNDPLTGAAPSLQNVNQRVIIAPGVLSPQSDLIGSDFRSWYHSWQVQAIKRF
                     SRGFSINASYVLSKSIDTMSSNVFSNLLRNPFNIRDNRGRSDFDQRHAVIASWLWSPS
                     FRFSHVLARAILGDWTVSGLHTFSSGAPMSFSMGDDVALDGTGNSQNAQFKPDAGPLQ
                     RDHASRADMIARFFNTEVFVPTGQVPRGIYGNAGRNILSGPASANTDVAVMRDFPLRE
                     RTRLQFRGEFFNLLNQVNFAQPNTTVNSSAFGTIRSAGAAREIQFALKLMW"
     gene            3579..4223
                     /locus_tag="VFQ79_03175"
     CDS             3579..4223
                     /locus_tag="VFQ79_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0138680.1"
                     /translation="MRIATILISSLLAAAAVTPGQQRAAPQVLAPEWVQLFNGKDLAG
                     WKKQGNEKWVVEDGTIYGEGITKEYGYIATERHYQDFHLFLRFKCEADGNSGVYIHTT
                     FDGDTAKHIGGRQIEIDRRIGRHTAGLYGDGKGWIVWPAPELETVIRPYDWNDLLIQV
                     EGNRYRTWLNGVRMIDFTYPSPATTDGVIALQLHSGGEGRMRFKDIWVRDLRRR"
     gene            4226..5344
                     /locus_tag="VFQ79_03180"
     CDS             4226..5344
                     /locus_tag="VFQ79_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138681.1"
                     /translation="MHDSSRRDFVFASFTSLLARGRAVAAEDAHGTAMIGTGNRGSYL
                     LKTVLQEPRARVAAVCDIKPDRLDTAATTAARDKPATFTDYRKLLERKDVEAVFIAAP
                     CDLHVEMAMAALRAGKHVYCEKPLGVTPESINDLLQVARSSTTVFMVGHQRRSDERLQ
                     KGVARIHEGILGRPVMMKAQRHSAGDFAHDGPSADWIFDAKRSGDVIVENAVHNLDVC
                     NWAVNSRPERAAGFGGTLLWVNQPPGRTTTDGYTLSYEYANAVELSFTQVAFHPAGLP
                     SGGQQTFVYGTEGAVDLEAATYYPRARGAKPQVLAEPFRGNREARHIGIFYDCIRTGR
                     KPEVDIVVGATAALTAILGREAYRRKKVVTWKELGVEV"
     gene            5334..6263
                     /locus_tag="VFQ79_03185"
     CDS             5334..6263
                     /locus_tag="VFQ79_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0138682.1"
                     /translation="MKSDAALFDRRQFVAVAPLAMVPGWGAAPPSSPARFVKGVCAGI
                     FPRNMPNAERCRRAKNAGFDAIEFPLSGEITLNSSPDEVKRLGDAAHKAGVAIASLWV
                     SPLGSTPLNDPDPAVRSRGIEAIRKAIEFAGYLSCDALLVVPGRVGSGAKFQVGYQVT
                     WDRISAEFPKAIPFAEKARVHLTLENVSNRFLVSPLEMRAFVDQFRSPWVGAHFDIGN
                     VMYFGYPQDWILTLRDRIRRVHLKDRKVSPRAEQRQPSGLLEGDVDWEQVMAALVKVG
                     YRGYACAEIGHDPNDPDQLKNLSAAVDKIFALA"
     gene            6454..6852
                     /locus_tag="VFQ79_03190"
     CDS             6454..6852
                     /locus_tag="VFQ79_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0138683.1"
                     /translation="MPLYDQVVYAAKRALVSGLLRPGDPFPSVRTLSAELKINPNTAH
                     KVVAALVNDGLLEVMPGSGTVVARPPASTAAERSALLEDQLEQLVVEAKKLGLHLADV
                     SKALARHWERLDGGAKGGNASAGRGARRSL"
     gene            6849..7751
                     /locus_tag="VFQ79_03195"
     CDS             6849..7751
                     /locus_tag="VFQ79_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0138684.1"
                     /translation="MNFPIRTYSIWKQFRGTTVLEDLCLNVPEGSIYGLVGPSGAGKT
                     TAIKLLMNILRPSAGWSEVFGVDSHRLSPREFAQIGYVSENQEIPDWMTVGYFLKYWR
                     PFYPTWDCDLAEELARQFQLPLNRKLRHLSRGMCIKAALASSLAYRPKLMILDEPFTG
                     LDALVRDELIAGFLPRAENSTILISSHDLAEIETFASHIGYLEHGRLQFSEDLDTLRQ
                     RFREIVLTFPSAPVMPEEWPANWLGVEAAGSMIRFVDARFSEERTPETVRHVFPDVRE
                     IGVNGMPLRSIFVTLAKAGRPTAA"
     gene            7762..9273
                     /locus_tag="VFQ79_03200"
     CDS             7762..9273
                     /locus_tag="VFQ79_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138685.1"
                     /translation="MKQVLAIFRKDILRLRLQLAGALAFTVLIAYVDRPDAGGFVSNL
                     AYMLWIACWVYIGASLIHQEHLPGDRQYWLTRPYDWRRLLAAKVLFGITFAVLPLITI
                     KMAVLWVNGIPPLRHAPAILSNSLAFMGAVGLFAGSLAAVTATLTQFLWGLLAISALG
                     GVAIALGVDYGGDWGAISWIRSATVYLSLAAAGALVLLLQYWLRRTFASRVILSLAVL
                     IAAVAPFGNAWHGAWRLSSSAPESLPVRLSFDSTPRARMQYADAPMVVAPGYRGIYLP
                     VRVSGIPSGSAMVSQRVEVTLAGSSGTLWTSGWRREGAVIGMDRLADVRFIRADGPAW
                     QYLDIDRAIYETIKDVPVQINVSIALLLLSDRRTGSMPGFGRTERLPLDGICDARPVP
                     EFSRSIRAARDVRNAEMSCAWPKPGPERAYMRVTSPPGVEDSHALLTTHSDGVLSIDQ
                     PVWRRVRAVLAFRGAEPRFTLETWRASERLDRSFQISGVRLRDYAAPRATDPQ"
     gene            9280..10707
                     /locus_tag="VFQ79_03205"
     CDS             9280..10707
                     /locus_tag="VFQ79_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138686.1"
                     /translation="MYRIFAIFRKDARHLWLQIAVLAMLMALVAWLDPTYRGDKASYY
                     DLLFSFALPLTCGLIVISVIHEEKLPGDRQYWLTRPYSRTELIAAKVLFMAVFINLPL
                     LAHHICVYAALGIPPQNHIGALLWKQVFFTAFYILPAAGLAVITRTLGGTLVVLLLGG
                     LALWIADMAFQFIAGRPLFLLQLPGRGATIVQALLLVAGVAVIVVLQYTKRRTAVAGV
                     LAAAITLAVALCSVYGTAYGRQSQPIRTGTVRLSLDSDPSRRSSMKPGGDPDMKTVDI
                     PVRLNGAPAGIVLDRYLHVVVIAPGNRFLFALQGILHSAESGRAWLSFTAEGYQFERT
                     VGQPVSLSGSFDLQVFDSPVVLPLPKRSTVVAPGVGACHDSLGPNGEVSFTCYSPSPR
                     AALMASNLRRRTNWIISPGSVESSLPTASGFQPVRKFVSLLSYRNWDQTEGLKLIAAR
                     PRPPIRVEFDLSPISLRTYLVSGNK"
     gene            complement(10708..13173)
                     /locus_tag="VFQ79_03210"
     CDS             complement(10708..13173)
                     /locus_tag="VFQ79_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138687.1"
                     /translation="MQGIQDVRYALRLFRKNPGFTAIAVLSLALGIGANTAIFSLIDA
                     VLLRPLPVPDPDRLVAIYHRDLGRGGLSSSSYPDYEFYRDQNRVFSGMLAYLRVPMNL
                     TAGDASERLSGELVSPDYFSTLGIQAQRGRVIQASDVSAKGRNAVAVISHNFWQRHFA
                     GAEPAIGSTLTIARHPFTVIGVMPESFRGLVLDWSKPPDVWIPATMYAEAVPPLEPAP
                     VLTGWGFHSFLVMARLKPGVSLEQAQAALSVLSGQAASQRSQVFRDMRFTAEVYPAQR
                     ARFWPSYRGPIIQFLTMLGAVVGLVLLAACFNLANLSLARAFERRQEIAVRLSIGAGR
                     GALIRQMLIESLLLAIFGGVAGLLMAELIMRFLQGFPTPFRIPIAFDAVLDWRVLLFT
                     FALSLLTGIAFGLAPALRGTRTDLSVALKGAGDGRSSRGGERLRGILVCAQVALCVLL
                     LAGAGLMVRTLRNAIASDVTADPSNVLLVSFDLASAGYDNTRAGAFYSALQDRVEALP
                     GVRRASLVQTVPLGGYRGGSDVLIRGVNDAAEQRLQVDFNVVSHGYFETVGLPLVQGR
                     FFTRRDRGGAPRVAVVNEVWAQRFWPGENAIGRSFRLTEPGSPVVEVVGVVRDGSFRN
                     LREPVRACFYVPLAQHPGRGMNLQVRTAGDALAFVGAVKQEIRALDQNIIPGDFLTLE
                     SHRNASLSQERLSASLLSGLGVLALVLAAIGIYGVLSFTVAQRNREIGIRIALGAQTA
                     QVVRTVLGESLRMVVLGLALGVVGALWLTRFIRSLLIGVDPADPLTFGAIVILLGTVA
                     AVAAFIPARRAARVDPMVALRQQ"
     gene            13241..13897
                     /gene="msrA"
                     /locus_tag="VFQ79_03215"
     CDS             13241..13897
                     /gene="msrA"
                     /locus_tag="VFQ79_03215"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0138688.1"
                     /translation="MHFTRSDIRNFAVLLLGAASGLTAAAFPEPAIDVKPRPGKQTAV
                     LAGGCFWCTEAVFEIIEGVEDVISGYSGGTRETAKYDIVSTGRTDHAEAVLITFDPAK
                     TSYGQLLQIFFSVAHDPTTLNRQGPDVGPQYRSAIFYADDEQKRVAEAYIKQIDAAKV
                     LGRPIVTQVVPLDKFYTAEKYHQDYAGKNPSNPYIVNVSDPKVEKLKKLYPGCVRKKK
                     "
     gene            13945..14151
                     /locus_tag="VFQ79_03220"
     CDS             13945..14151
                     /locus_tag="VFQ79_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU0138689.1"
                     /translation="MPISARNQLEGKVVEVKLGDIMAHVVVRVGKNQIESVITRRSAE
                     QLGLKKGDRVTVVIKSTEVMLEKS"
     gene            complement(14148..15230)
                     /locus_tag="VFQ79_03225"
     CDS             complement(14148..15230)
                     /locus_tag="VFQ79_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnH family di-heme enzyme"
                     /protein_id="HEU0138690.1"
                     /translation="MTGRAVSFALLAAISLSAAGWEWTVPAGFPKPRVPADNPMTAAK
                     VELGRHLFYDKRMSVNGKQSCASCHKQELAFTDGRSHAEGTTGQLHPRSSMSLVNVAY
                     APALTWAHPALDSLEEQALVPMLGEDPVELGLRGREQQFVDAMKQDPVYRRLLPQAFP
                     GEREVTLQTVTKALAAFQRSIVSMRSPYDRYRWGGEPDAISDSAKRGELLFSSSERAG
                     CFQCHGGWNFSSVRFDGPGEAIDRNGFFFNTGVSTYEPPNRGIHEHTHRPEDVGKFRA
                     PALRNIAVTAPYMHEGSLPTLEAVIDHYAAGGRMEHANKTPILRRFHLTDDEKRDLIE
                     FLKTLTDEELLRDPRWSDPWPRSGKR"
     gene            complement(15227..16357)
                     /locus_tag="VFQ79_03230"
     CDS             complement(15227..16357)
                     /locus_tag="VFQ79_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0138691.1"
                     /translation="MLPKSTIENRLAQIRKSRGIGASDLARRVNVSRQTIYAIECGTY
                     VPNTEVALRMARELEVTVDELFSLSPTAPNAPESMSAEVLSASKLAKGQPVRICQIGS
                     RWVGVPVPSTPYFMPEADGIIQRTGRTSGRADLMVFGKDEAAQKRLLLAGCDPATSLL
                     SNMVERISGVEIVSAAASSKLALTWLKEGKVHVAGSHLEDPKSGEFNLPYVREKFTGE
                     DILVVTFARWEEGFVVSSGNPKGIRKAQDLARKGVRFVNREAGSGSRALLDKLLSDAG
                     VPSSKVQGYDRIAFGHLPAAFHVLSGEADACLATRSAAQTFGLDFVPLHSARYDLVMR
                     KRTGDLPSVKAFLDVLQRTTLRRKLEVLAGYDTSQTGAQLTV"
     gene            complement(16357..16845)
                     /locus_tag="VFQ79_03235"
     CDS             complement(16357..16845)
                     /locus_tag="VFQ79_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0138692.1"
                     /translation="MYPRHLFLSFLLICGGLVAQEAPPSIQVTGAVKQPLTLTAEDLA
                     KMPRASVKTAGQGVETVYEGVWVHEILKRAGAPQGQALRGKALAGYVLAEAQDGYQVV
                     FSLGELDPAFVDNEILLADTANGKPLFGAQGRFRLIVPKDKPGARSVRMLTKLEIVQL
                     RK"
     gene            complement(16858..>17041)
                     /locus_tag="VFQ79_03240"
     CDS             complement(16858..>17041)
                     /locus_tag="VFQ79_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138693.1"
                     /translation="AGKTFTITERFQTEFRAEAFNVTNTPPLGNPNGSFGNAAFGSIT
                     TALDPRVFEFVLKLHF"
BASE COUNT         3424 a         5228 c         5054 g         3335 t
ORIGIN      
        1 cctcccacca ggtgatcctc ttggccatcg ccttcaacag cacgtcttgt agtttcatca
       61 tccgctccac ttccgcggcc gagtatcgct ggctcgtttt caccgctcca gcgtctaccg
      121 ccgctcagct cgaacggaca gctcatgtgt gaattggacc ggacagatca catactaacg
      181 acacaggcct ggagcactgg ttgactcggt gcgcggtggg atgtagaatc gccgcgttgg
      241 gaaaccgcaa gtggaaatcc ttggcataat gaacctcccg gaaacgtagc caaccctgca
      301 cctccgggtg gatggggggg aagatgatcg cacgccgagt cttgattctc ttcgtcacag
      361 cagcagtttg tgtcccggtt gtttcaacag gccagactac gggaactatt agcggctata
      421 tcaccgatcc ggcaggagca gtagttcaga cccaggtaac ggcagtcctg gtgcagcaga
      481 atgtctcgcg aacggtcgag tcgaataccg agggattcta tatcttcaac gcgctgccgc
      541 cgggcactta caacctctca gcgcagcctg ccggtttcca acgcttcgtt cgcagcgggg
      601 tcactctcac ggttagccag aacgtgcggg cggacatcag gcttcagttg ggcgcagtgt
      661 cggaaacagt agaggtcagc ggcgaagctc cccttgtgga tactacgtcg ggcaccatat
      721 ccggactcgt ggacgatcgc cgcgtcgtgg atctgccgct caacggcagg aacattatcg
      781 cgctggcgag gaccgtgccg ggagtccaga acgtcaaggc gccgcagagc ctcaacaatg
      841 cgcgcgacgg ggcgctgatg aacgtcaacg gcagcgcgtc caccatgaac gtgtttacgc
      901 tgaacggcgc caatttcatc aatccgtcgc gaaacacggg gctcaactac ccgccgcccg
      961 atgcgcttca ggaatttcgc atccttacta acagcttttc cgccgaatat gggcggaatg
     1021 cgggatcgca ggtcaatgtg gtttccaaag ccggaacgaa tgcattccac ggggcgttgt
     1081 gggagttcct gcgcaacgac aatctgaacg ctcggaattt cttcgcggcg cgtaggcctg
     1141 cgcgggtgca gaaccagttt ggcggagccg ccggagggcc ggtcgtgcgc aacaaggtgt
     1201 tcgtgttcgg cacctatcag ggcctgaggg accggccgga ggcggtcagc gcccaggcga
     1261 tcgtgcccag caccgcccag cgcagcggcg atttcagcgc ctcgagcaag gttctccgga
     1321 acccgatcga cccgctgacc ggagcgcctt tcacagaccc tgccgggctt ccctgcgtcc
     1381 agagcaacat catccgcccg gggtgcatca gcccggcgat ctcgaaacac tttcttccgc
     1441 ttgtgcccga atcgcccacg ggaacggtga caaccctgtc tcccagcccg atcaacgcgg
     1501 acctgttcat ggtgcggacg gactggctcc agagctcgaa gaacaccctg tttacgaact
     1561 atttccgcga ccacaacctg cgcgaccgtc cgacgttggc cgccggtaac atacccggct
     1621 tccaatcgag caaagatgtt accgacacgc acctggcggt gtttagcgac acctatacgg
     1681 tcagccctac tcttctgaac gaattcaagg tatctttcct gaggagcacc tccgaaagcg
     1741 gcgcatcgcc gaccatcgaa cctcgggatc tgggtatcaa tatgttcaag taccccgccg
     1801 gcggagcgct ttcgctgagc gtcggcagct tgttcagttt cggcagcgcg ggcaaggttg
     1861 tcttcaccag caataactgg caattccgcg acacagtaag ttggatcaaa ggccggcaca
     1921 atgtcaaatt cggtggcgaa ctgctgcgca tgcatttccg acagatcttc ctcggtccac
     1981 cgggcttcag cttcaacgga tcacgcagcg gcgacgagat cgccgacttt ttgctgggcg
     2041 cctttcgcac actgagccac ggtttcggcg tacgggagaa cgacgatttc atgactgcgc
     2101 cgaacttctt cttccaggac gagttcaaga tacacccccg gttcaccctg acgtacggtg
     2161 tccgctacga gccctttctg ccttgggtgg accggtacga ccgtctcgtc agcatcgaag
     2221 gtgtcttcag cggacagcgc tccgagcgct tccccgacgc gcctccgggg atcgtatacc
     2281 ccggcgatcc cggcgtgagc agggggatcg tccacaacga cctcaacaac tttgcgccgc
     2341 gcttggcgtt tgcctgggat ctgttcggcg atgggaagac cagcgtcagg ggcgcatacg
     2401 gcgtattcta cgatcacatc aaggccgatg cagtggcgca ggagaacgcg ccatgggccg
     2461 gcagttcgca aatctttgac ggccgtgtgg acgaccctta tggttcgctc ggcctcacgc
     2521 cgccgccggt tgtgcccggt ggcgaggact tcgcgcgttt cccgctcccg atccggtatc
     2581 gcggcatctc cgacacgctt cgcaccgcct acgtgcaatc ctggaatgtc accatccagc
     2641 gacagctcac cgggagcgtg atgctggaaa cctcttatgt cggcaaggcc gggatcaaga
     2701 tcgagggcgc gcggaatttc aatccggcag catttaagaa cgatccgctg acgggcgccg
     2761 ccccgtcgct ccagaacgtc aaccagcggg tgattatcgc gccgggcgtc ctgagtcctc
     2821 agagcgacct gatcggcagc gatttccgca gttggtatca cagttggcag gtgcaggcga
     2881 tcaaacgttt cagtcgggga ttctcgatca acgcatccta cgtgttatcg aagtcgatcg
     2941 acaccatgtc cagcaacgtc ttctcgaacc tgttgcggaa tccgttcaat attcgcgaca
     3001 accgcggacg ctccgatttc gatcagcgcc atgccgtgat cgcatcctgg ctctggtcgc
     3061 cctccttccg cttttcccac gttttagcgc gagcgattct cggcgattgg acggtctccg
     3121 ggctgcacac cttcagcagt ggcgccccca tgagcttttc catgggcgac gacgtcgctc
     3181 tcgatggcac gggcaatagc cagaatgcgc agttcaagcc ggatgccggt ccgcttcagc
     3241 gtgaccacgc gagccgtgcc gatatgattg ccaggttctt taacacagag gtctttgttc
     3301 caaccgggca ggttccccgt ggaatctacg gcaacgccgg ccggaatatt cttagcggtc
     3361 cggcatccgc caacaccgac gtcgctgtca tgagggattt tccgctgcgg gagcgcacgc
     3421 gattgcagtt ccggggagag ttcttcaacc ttctgaacca ggtgaacttc gcgcagccga
     3481 ataccaccgt caattcgtcc gcgttcggca ctattcgcag cgccggggcg gcgcgcgaaa
     3541 ttcagtttgc gctgaaactg atgtggtaag agaccctcat gcgaattgcg acaatcctta
     3601 tttcctccct gctggcggcg gccgccgtga cgccgggaca acaacgtgcc gcgccacagg
     3661 tactggctcc cgaatgggtg caactcttca atggtaagga tctcgccggc tggaagaagc
     3721 aggggaatga aaagtgggtc gtcgaagacg gcaccattta tggcgaggga attacaaagg
     3781 aatacggcta cattgcgacg gaacgccact accaggattt ccacctgttc ctgcggttca
     3841 agtgcgaggc cgacggcaac agcggagtct atatccatac cacgttcgac ggcgacaccg
     3901 ctaaacacat tggcgggcgg cagatcgaaa tcgaccggcg catcgggcgg cacaccgccg
     3961 gcctgtatgg cgacggcaaa ggatggatcg tctggccggc tcccgagttg gaaaccgtca
     4021 tccggccgta cgactggaat gatctgctga tccaggtgga gggcaaccgc taccgcacct
     4081 ggttgaacgg agttcggatg atagatttta cgtacccttc gccggccacg accgacgggg
     4141 tgattgcgct gcaactccac tccgggggcg aagggaggat gcggttcaag gacatctggg
     4201 tacgggatct gaggaggcgc tgaccatgca cgattcatcc agaagagatt tcgtttttgc
     4261 ctcgttcacg tcattgctcg cccgcgggcg cgctgtggcc gccgaagatg cgcacggcac
     4321 cgcaatgatc ggcaccggaa accgtggttc ttacctgctc aagacggttc tccaggagcc
     4381 gcgggccagg gtggcggccg tctgcgacat caagccggac cgcctggaca ccgccgctac
     4441 aaccgccgcc cgggacaagc cggcgacgtt caccgattac cgcaagctgc tcgaaaggaa
     4501 agacgtcgag gccgtcttca tcgccgctcc ctgcgacctg cacgtggaga tggcgatggc
     4561 cgcgctgcgc gccggtaaac atgtgtactg cgaaaaaccg ctcggcgtca cgccggagtc
     4621 cattaacgat ctgctgcagg tggcgcgatc ctcgacaacc gtcttcatgg tgggccacca
     4681 gcgccgttcc gacgagcggc tgcaaaaggg tgtcgcccgc atccacgagg gcattctcgg
     4741 caggccggtc atgatgaagg cccagcggca ttccgctgga gacttcgcgc acgacgggcc
     4801 ttcggcggac tggatctttg atgcgaagcg ttccggcgac gtcatcgtcg agaacgcggt
     4861 tcataatctt gacgtctgca actgggctgt gaacagccgt cctgagcggg ccgccggttt
     4921 cggcggcacg ttgctgtggg tgaaccagcc tcccggcaga accaccaccg acggttacac
     4981 gctgagttac gaatatgcca acgccgtcga gctgtctttc acgcaggtgg cctttcatcc
     5041 ggccggattg ccgtccggcg ggcagcagac tttcgtttac ggcacggagg gcgccgtcga
     5101 cctggaggcc gccacctact atccgcgggc gcgcggtgcc aaaccgcagg tgctcgccga
     5161 gcctttccgt ggcaaccgcg aggccaggca catcggcatc ttctacgatt gcatccgcac
     5221 cgggcgcaaa ccggaagtcg atatcgtggt cggtgccaca gccgcgctca ccgcgattct
     5281 tggccgtgag gcataccgcc gcaagaaggt ggtcacctgg aaggagcttg gagttgaagt
     5341 ctgatgctgc tctctttgat cgccggcagt tcgtggccgt agcgccgtta gcgatggttc
     5401 ctggctgggg cgcagcaccg ccgtcgtctc cggcgcggtt cgtgaaaggc gtgtgcgcgg
     5461 gaatcttccc gcgcaatatg cctaacgccg agcggtgccg ccgggcgaag aacgccggat
     5521 tcgacgcgat tgagttcccg ctcagcggcg aaatcaccct gaactcatcg ccggatgagg
     5581 tcaagcgcct gggcgacgcg gcgcataagg cgggtgtcgc aattgccagt ctttgggtgt
     5641 cgccactcgg ttccacaccg ttgaacgatc cggacccggc cgttcgcagc cgcggcatcg
     5701 aagccatccg taaggctata gagtttgccg gctatctgag ttgcgatgca ctgctggttg
     5761 tgccgggcag ggttggctca ggggcgaaat ttcaggttgg gtatcaggtc acctgggatc
     5821 gcatttccgc cgagttcccg aaggccatcc cctttgcgga gaaggcgagg gttcacctca
     5881 ccttggagaa cgtttccaac cgattccttg tcagcccgct ggagatgcgt gcatttgtgg
     5941 atcagttccg gagcccatgg gtgggagcgc atttcgacat cggcaacgtc atgtacttcg
     6001 ggtatccgca ggactggatt ctgactctcc gcgatcgcat caggcgcgta cacctgaaag
     6061 atcggaaggt atcccccaga gccgaacaga gacagccgag cggattgctc gaaggcgatg
     6121 tagattggga acaggttatg gcggctctcg tcaaagtggg ataccggggc tatgcttgtg
     6181 cggagatcgg ccacgacccc aacgatccag atcagttgaa gaacctctcg gccgcagtcg
     6241 acaaaatttt tgcgctggca tagcgtgcag tggggcagca aacgagagtt ttgtttgccg
     6301 atagtcaggg tatgtcctcc ttccgatgcc aagccgatcc agttgagctt aacgggagat
     6361 caacggacat agcagacggt agcgcttgcc agctactgta ctatctgtaa taatacacct
     6421 gtgatacctt tccgggttga gttccggccg ggaatgccgc tctacgatca ggtggtttac
     6481 gccgccaaaa gggcactcgt ttcggggctc ctgcggcccg gagatccgtt tccctcggtt
     6541 cgcaccctta gcgcggaact gaaaatcaac cccaacaccg cgcacaaagt tgttgccgcc
     6601 cttgtgaacg acggcctgct ggaagtgatg ccaggctccg gcacggtggt ggcacggcca
     6661 ccggcctcca ctgcggcgga acggtcggcc ttgctggaag atcagttgga gcaactcgtg
     6721 gtggaggcaa aaaagctggg gttgcacctt gcggacgtat ccaaagcgct tgcccgtcat
     6781 tgggaacggc tggacggtgg ggcgaaaggc ggcaatgcat cagccggcag aggagccaga
     6841 cgaagtctat gaactttccc attcgcacct acagcatctg gaagcagttc cgcggcacca
     6901 cagttctgga ggacctctgc ctgaatgtgc cggaaggcag catctatgga ctggtcggcc
     6961 cgagcggagc cggaaaaacc actgccatca agctgctgat gaatatcctg cgcccctcgg
     7021 ccggatggtc ggaggtgttc ggcgtcgact cgcatcgtct ttcaccgcgc gagttcgcgc
     7081 agatcggtta cgtttccgaa aatcaggaga tacccgactg gatgactgtc ggatactttc
     7141 tgaagtattg gcgccctttc tatcccacct gggactgcga tctggcagaa gaactggcgc
     7201 gccagttcca actcccgctg aaccgcaagc tgcgccacct ctcgcggggg atgtgcatca
     7261 aggccgcgct tgcctcatcg ctcgcgtacc gtccaaaact gatgatcctg gacgagccgt
     7321 tcaccggcct cgatgcattg gttcgcgacg agttgatcgc cggatttctg cctcgtgcgg
     7381 agaactccac aattctcatc tcttcgcacg atctggcgga aattgagacg ttcgcgagcc
     7441 atatcggata cctggagcac gggcggctgc aattctccga agacctcgac acactccgcc
     7501 agcgatttcg ggaaatcgta ctgaccttcc cttcggctcc cgtaatgccg gaggagtggc
     7561 cggcgaactg gctcggagtg gaggccgcgg gctcaatgat ccgattcgtc gatgcgcgat
     7621 tcagcgaaga gcgaactccc gaaacggtgc gtcatgtgtt tcctgatgtt cgcgagatag
     7681 gcgtgaacgg aatgccgctg cggtccatct tcgtcacgtt ggcgaaggcg ggccgtccga
     7741 ctgccgcgtg aggagcgccg ggtgaaacag gtattggcaa tcttccggaa agatatactg
     7801 cggctgcgtc tgcaactggc cggcgcgctg gcgttcactg tactcatcgc gtacgtggat
     7861 cgtccggacg ccggcggttt tgtatcaaat ctggcctata tgctgtggat cgcatgttgg
     7921 gtctacatcg gcgcttctct gattcaccaa gagcacttgc ctggtgaccg gcagtattgg
     7981 ctcacgcgcc cgtacgattg gcggcggctg ctggcggcca aagtcctgtt tggaattaca
     8041 ttcgccgttt tgccgctaat cacaataaag atggcggtcc tgtgggtaaa tggcatccca
     8101 ccgctccgtc atgctccggc aatcctttcc aactccttgg cgtttatggg tgcggttgga
     8161 ttgttcgccg gctctctcgc ggctgttacc gcaactctca ctcagttcct gtggggcctg
     8221 ctcgcaatat cggcattagg aggagtagcc atcgcgctcg gtgtggatta cggaggcgat
     8281 tggggagcga tctcctggat tcgctcagca accgtttacc tgtcgctcgc cgctgccggc
     8341 gctcttgttc tgttattgca gtactggttg cggagaacgt ttgcttcccg cgtcatcctg
     8401 tcgctagcgg tgctgatcgc cgccgttgcc ccgtttggga acgcctggca cggcgcctgg
     8461 cgcttatcct ctagcgcacc agaatcgctt ccagttaggc tttcgttcga ttccacacct
     8521 cgggcgcgaa tgcagtacgc ggacgcgcca atggtcgttg cccctggtta cagggggatt
     8581 tatcttccgg ttcgtgtgag cgggattccc agcggtagcg cgatggtgag ccagcgagtt
     8641 gaggtgacgc tggcggggag cagcggaaca ttgtggactt cgggatggcg gcgggaagga
     8701 gcggtgattg gcatggaccg attggccgat gtgcgtttca ttcgggcgga tggtccggcc
     8761 tggcaatacc tggatatcga tcgcgcaatc tacgagacca taaaagacgt ccctgtacaa
     8821 atcaacgttt cgattgccct cctgctgcta agcgatcgcc gtacgggaag catgccgggc
     8881 tttggccgga cggagcggct gcccctggat ggaatctgcg acgcgaggcc ggtgccggaa
     8941 ttctcgcgct ctatccgcgc cgcccgcgat gttcgcaacg cggaaatgtc ctgcgcctgg
     9001 cccaagcccg gcccggagcg ggcttatatg agggttacgt cacctcccgg agttgaagac
     9061 tcccatgccc tgctaactac ccactcagac ggcgtgcttt ctatcgacca gccggtatgg
     9121 cggcgcgtgc gcgccgtcct cgcattccgc ggtgccgaac cacgattcac cctggaaaca
     9181 tggcgtgctt cagagcgcct cgaccgtagc tttcagatct ccggcgtgcg gctgcgggac
     9241 tatgccgcgc cgcgcgctac cgacccgcag tgaggaacca tgtacagaat atttgcgatc
     9301 ttccggaaag acgcccgtca cctttggcta cagatcgccg tattggccat gttgatggcg
     9361 cttgtggcct ggctcgatcc gacttatcgc ggtgataaag cgtcttacta cgacttactg
     9421 ttttcattcg ccctgccgtt gacatgcggc ttgattgtga tctcggttat acacgaagag
     9481 aagcttcccg gcgatcgcca atactggctg acccggccgt attcgcgaac agaattgata
     9541 gcggcaaaag tcctgttcat ggccgtattc atcaacttgc ctctcctggc gcaccatatc
     9601 tgcgtatacg ctgcgcttgg aattcccccg caaaatcata ttggggcgct gctttggaaa
     9661 caggtctttt tcacggcgtt ctacattctt ccggcggccg gtcttgccgt aatcacccga
     9721 acgctcggcg gaacgctggt ggttttgcta ttgggcggct tagctctttg gattgcggat
     9781 atggccttcc agttcatcgc agggcgtccg ctatttctct tgcagcttcc cggccgcggc
     9841 gcaaccatcg tgcaggcact gttactggtt gcgggcgttg cggtcatcgt ggtcctgcaa
     9901 tacaccaagc gaagaacggc tgtcgcaggc gtactcgcgg cggcaatcac actggccgtg
     9961 gctttgtgca gcgtgtatgg cacggcgtac ggccggcaat cgcaaccgat ccgcaccggg
    10021 acggttcgcc tttcgcttga ttccgaccca agcaggcgta gcagcatgaa accaggcggc
    10081 gacccggaca tgaagacggt cgacattccc gtccggctga acggggcacc ggctggcatc
    10141 gtattggatc gatacctcca tgtagtggtt atagcgccag gaaaccgttt cctatttgcc
    10201 ctacaaggga tactgcacag tgctgaaagc ggacgcgcct ggctatcctt cactgccgaa
    10261 ggttaccagt tcgagcgaac cgtgggacaa ccggtctcac tgtccgggtc tttcgatctc
    10321 caagtgtttg acagcccggt cgtgctgccg cttccgaaga gatccacagt ggtggcgccg
    10381 ggagtcggag cctgccacga ctcactcgga ccgaatgggg aagtgtcatt tacctgctac
    10441 tcgccatcac cccgcgccgc actcatggcc agcaaccttc gaaggcggac gaattggatc
    10501 atctctcccg gatcggtcga aagctccctc ccgactgcga gtggttttca accggtgcgg
    10561 aagtttgtga gcctgctgtc ctatcgtaat tgggatcaga ccgagggact gaagctgatt
    10621 gctgcccggc cgcggcctcc aattcgagtg gagttcgacc tctctcccat ttcgctacgg
    10681 acctatctgg tatccggcaa caagtgatca ctgctggcgc agcgccacca ttggatcgac
    10741 ccgggctgcg cggcgcgcgg ggatgaacgc cgctactgcc gccacagtcc ccagcaggat
    10801 cacgatcgcg ccgaacgtca acgggtccgc cgggtctact ccaatcagca ggcttcgaat
    10861 gaagcgagtg agccagagcg cgccaacgac tcccagggcc aagcccagca caaccatgcg
    10921 gagcgattcg ccaagaaccg ttctcaccac ttgtgcggtc tgcgctccga gagcgatgcg
    10981 gataccgatc tcgcggttcc gctgcgccac cgtgaacgac aacactccat agatgccaat
    11041 cgcggccagc accagggcca acaccccaag tccgctcagc agagatgcgg acaaacgctc
    11101 ctgagaaaga ctggcgttgc gatgcgattc cagtgtcagg aaatcgcctg gaataatgtt
    11161 ctgatcgagc gcgcgaatct cctgcttaac cgcacccaca aacgccagcg catcgcctgc
    11221 tgtcctgact tgcaagttca ttcctctccc gggatgctgc gcgaggggca cgtaaaagca
    11281 ggcacggacc ggctcgcgca agttgcgaaa actgccgtcg cgcaccacgc ccacgacttc
    11341 caccacagga ctaccgggct cggtgagccg gaaagaccgg ccgatcgcgt tttccccagg
    11401 ccaaaaccgc tgcgcccaaa cctcgttcac caccgcgacc cgcggagctc caccgcgatc
    11461 cctccgcgtg aagaaacgcc cttgcaccag gggaagcccg acggtctcga aatagccatg
    11521 cgagacgacg ttgaagtcca cctgcaggcg ttgctcggcc gcatcattca cgccgcggat
    11581 cagaacatcg gagccgcccc ggtagccacc cagcggaacg gtctgcacca gggatgctct
    11641 acggacaccg ggtagcgcct ccacacggtc ctgcaacgcg ctgtagaagg caccggcgcg
    11701 agtgttgtca taacctgccg aagccagatc gaaggaaacc agcaacacgt tcgaaggatc
    11761 ggcggtcaca tccgacgcaa tagcattgcg caacgtccgc accatcagac ctgcgccggc
    11821 cagcagcagc acacaaagcg ccacctgtgc acagaccagg atgccacgca atctctcgcc
    11881 gcctcgtgac gatcggccat ctccagcacc cttgagcgct accgatagat cggtccgtgt
    11941 gcccctgagc gccggcgcca atccaaacgc gattccggtg agcaacgaca gggcgaaggt
    12001 aaacagcagc acccgccagt cgagaactgc gtcgaaggcg atcgggatgc gaaaaggagt
    12061 cgggaaccct tgcaggaatc gcatgatcaa ctcggccatc aacaagccag ccaccccgcc
    12121 gaaaatagca agcagaaggc tttcgatgag catttggcgg atcagcgcac cgcgcccggc
    12181 accgatcgag aggcgaaccg cgatctcctg gcgccgctca aaagcacgag caagcgacag
    12241 gtttgcgagg ttgaaacagg cggcgagcag aaccagtccg acaaccgctc cgagcatcgt
    12301 gaggaactga ataataggcc cacggtacga tggccagaac cgtgcgcgct gggcgggata
    12361 gacctcggcg gtgaatcgca tatcccggaa aacctgagac cgctggctcg cggcctgacc
    12421 agaaaggacg ctgagcgcgg cctgagcttg ttcgaggctt acgcccggct tcagtcgcgc
    12481 catcaccaga aacgagtgaa agccccaccc cgtgaggacc ggagcgggtt ccagaggcgg
    12541 gacagcctcc gcgtacatgg tggcgggaat ccacacatcc ggaggcttgc tccagtcgag
    12601 aaccaggccg cgaaagctct ccggcatcac gccgatcacg gtgaaggggt ggcgcgcgat
    12661 ggtgagagta ctgccgattg ccggctcggc gcctgcaaaa tggcgctgcc agaaattatg
    12721 actgatcacc gcaacggcat tccggccctt ggcgctcaca tcggacgctt gaattacccg
    12781 gccgcgctgg gcctggatgc ccagagtcga gaaataatcc gggctgacca gttctcctga
    12841 caggcgctcg cttgcgtctc ccgcggtgag attcatgggc acgcgcagat aagcgagcat
    12901 tccggagaac acgcggtttt gatcacggta gaactcgtag tcgggatacg aactcgagga
    12961 caagccgccg cgcccgaggt cgcggtgata gatcgcgacc agccggtccg gatcgggaac
    13021 cggcagcggg cgcagaagaa cagcatcgat caggctgaaa attgcggtgt tggctccaat
    13081 gcccagcgcg agcgacagga cggcaatcgc cgtgaagccc ggattcttac gaaaaaggcg
    13141 caacgcgtag cggacgtcct ggatgccctg catgccctca ggctatcgca actccttgcc
    13201 gtcacgagtg cgaaactgaa agagcacgag gggaatcgga atgcatttca cgagatcgga
    13261 catacgaaac tttgcagtac tgctgctggg cgcggcatca ggcttgacgg cggcggcgtt
    13321 cccggaaccg gccatcgatg tcaagccgcg ccccggcaag cagacggcgg tgctcgcagg
    13381 cggctgcttc tggtgcaccg aagccgtgtt cgaaataatc gaaggcgtag aagatgtgat
    13441 ttccggctac tccggcggca ccagggaaac agcgaaatac gacattgttt ccaccggccg
    13501 gacggatcac gccgaagcgg ttctgatcac cttcgatccg gccaaaacct catacggcca
    13561 gttgctccag atctttttta gcgtcgccca cgacccgact acactgaacc ggcagggacc
    13621 ggacgtgggt ccgcagtatc gcagtgcgat attttacgcg gatgacgagc agaagcgcgt
    13681 tgccgaagcg tatattaagc agatcgacgc agccaaggtc ctggggagac cgatcgttac
    13741 acaggttgtt ccgctcgaca aattctacac cgccgaaaaa taccatcagg actacgccgg
    13801 caagaacccg tccaacccct acattgtgaa tgtgtcggat cccaaggtcg aaaagctgaa
    13861 gaagctctac cccggttgcg tgcgcaagaa gaaatgagca cgcatccggt ggattcacgg
    13921 actcgatcgt ggcatgctta ggtcatgccc atatcggcgc gaaaccaact ggaaggcaaa
    13981 gtggtagagg tcaagctcgg agacatcatg gcgcacgtgg tcgtccgcgt cggcaagaac
    14041 caaatcgaga gcgtcattac caggcgcagc gccgagcaac tcggactcaa gaagggcgac
    14101 cgcgtaaccg tagtcatcaa gtcgaccgag gtaatgctcg aaaagagcta acgcttcccg
    14161 cttcgcggcc acggatcgct ccagcgggga tcgcgcagga gttcttcgtc ggtcaacgtc
    14221 ttcaggaatt cgatcagatc gcgcttttcg tcgtccgtca aatggaaccg gcgcaggatc
    14281 ggtgtcttgt tggcgtgttc catccgtccg cccgcggcgt agtgatcaat aacagcctcc
    14341 agcgtaggca ggctgccctc gtgcatgtac ggagcggtca cggcgatgtt cctcaacgcc
    14401 ggcgcgcgaa acttgccgac gtcttccggc cggtgcgtgt gctcatggat tccccgattt
    14461 ggaggctcgt aggttgatac gcccgtattg aagaaaaagc cattccggtc tattgcctcg
    14521 ccagggccat cgaaccggac ggaactgaag ttccagccgc cgtggcattg aaagcagcct
    14581 gcgcgttcgc tcgaggaaaa cagaagctca cctcgcttgg cggaatctga gattgcatcc
    14641 ggttcgcctc cccaacggta ccggtcataa ggagagcgca tcgaaacaat cgaccgttga
    14701 aaggccgcca gtgccttggt gaccgtttgg agcgtgacct cgcgctcccc ggggaaggct
    14761 tgcggaagaa gcctccgata cacggggtcc tgcttcatcg cgtcgacgaa ctgctgttcg
    14821 cgcccccgca aaccaagttc aaccggatcc tcgccaagca tcgggaccag cgcctgttct
    14881 tcgagggagt ccaacgccgg atgcgcccat gtcagcgccg gtgcataggc cacgttgacc
    14941 aggctcatgc tgctgcgcgg atgcaattgc ccggtagtac cttcggcgtg agaccgtccg
    15001 tccgtgaaag cgagttcctg cttgtggcag ctcgcgcacg actgcttccc gttcaccgac
    15061 atccgtttgt cataaaagag gtggcggcca agttccacct tcgccgcggt catcgggttg
    15121 tccgcgggaa cgcggggctt cgggaaaccc gcgggaaccg tccattccca tccggccgcg
    15181 gatagagaga tcgcagccaa cagcgcgaag gatactgccc ggccggtcat accgtcaact
    15241 gcgcaccggt ttgcgacgta tcgtaaccgg cgagcacctc cagcttcctc cgcagagtgg
    15301 tccgctgcaa gacgtcgaga aacgccttca cactcggcag atcgccggtc cgtttgcgca
    15361 tgacgaggtc atatcgcgcg ctatgaagag gcacgaagtc caggccgaat gtctgggccg
    15421 cagaccgggt tgcgaggcag gcatccgctt ctcccgacag aacgtgaaac gcagcgggca
    15481 aatgtccgaa cgcaatacga tcgtagcctt gcactttgga tgagggaact ccggcatcgc
    15541 tcagaagctt gtcgagcaat gcgcggcttc ccgatcccgc ttcccggttc acgaaacgga
    15601 cgcctttccg tgccaggtcc tgcgcctttc gaatgccctt cggattgccg gaagagacca
    15661 caaaaccttc ctcccatctc gcgaaagtca ccaccagaat gtcttcgccg gtgaacttct
    15721 cgcgcacgta gggcaaattg aactcgccgg acttcgggtc ttcgagatgc gagccggcaa
    15781 cgtggacttt cccttctttc aaccacgtca gagccaactt gctcgatgca gcggccgata
    15841 cgatctccac accgctgatt cgttccacca tgtttgagag caggctcgtg gcgggatcgc
    15901 aacccgcaag caggagccgt ttctgcgccg cttcgtcctt cccgaaaacc atgaggtccg
    15961 cgcgcccgct ggtccggccg gttcgctgga tgatgccgtc cgcctccggc atgaagtaag
    16021 gagtactggg tacggggacc cctacccacc gcgacccgat ctggcagatt cgcaccggtt
    16081 gtcccttcgc cagcttcgac gcgctgagca cttccgcgct catcgattcc ggtgcattcg
    16141 gcgcggtggg actcagcgag aacaactcgt caacggtaac ctccaattca cgggccatcc
    16201 gcagcgcgac ctcggtgttt ggcacgtagg taccgcattc aatcgcatag atcgtctggc
    16261 ggctgacatt cacgcgccgg gccagatccg aggccccaat cccccggctt ttgcgaatct
    16321 gagcaagccg gttctcaata gtcgatttag gcaacactat ttccttagct gtacgatttc
    16381 gagtttcgtc aacatccgca cggagcgcgc tcccggtttg tccttgggca cgatcagccg
    16441 aaagcgtcct tgcgcgccga acaagggctt cccgttagca gtatcggcta gcaggatttc
    16501 gttgtcgaca aaagccgggt ccagctcacc gagcgagaat accacttgat atccgtcctg
    16561 tgcctccgcg agcacgtaac cggctaatgc ttttccgcgc agcgcctgac cttgtggcgc
    16621 ccccgctcgc ttcaggattt catggaccca gacgccttca tacacggtct ccacgccctg
    16681 gccggctgtc ttcaccgacg ctcgcggcat cttcgcgaga tcctccgcgg tcaaggtgag
    16741 cggctgcttg accgctccgg taacttgaat cgacggaggc gcctcctggg ctaccaggcc
    16801 gccacagatc aaaagaaagg acaggaacag gtgtcttgga tacataacaa cctcacgtta
    16861 aaagtgtagc ttcagaacga actcaaagac ccgcggatcc agagctgtgg tgatggagcc
    16921 gaaagcggca ttcccaaagc taccgtttgg atttccgaga ggcggagtgt tggtgacgtt
    16981 gaacgcctcg gctcgaaatt cggtctggaa ccgctctgtg atcgtgaagg tcttgcctgc
    17041 c
//
LOCUS       DASTMK010000080         7407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_227727, whole genome shotgun sequence.
ACCESSION   DASTMK010000080 DASTMK010000000
VERSION     DASTMK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227727"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFQ79_03245"
     CDS             <1..760
                     /locus_tag="VFQ79_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU0138694.1"
                     /translation="ASPFFFLALFPALLQRMPKSGGWLARVKVVLGFIILAAMLKYIS
                     NVDQVLQWNLLTRERFLSAWVVLFALAGLYLLGLLRMEGVRPEDSLGVGRLLTAAALL
                     IFAISLIPGIFGARLGELDAYVPPPSAGSGLVAPGAAQDLAWMKNQYREALDRARSEN
                     KPVFVNFTGYACTNCHWMKANMFTRPQIASRLKDFVLVELYTDGTDAESEANQKLQES
                     QFSTVAIPYYAILDAGGKTIASFAGLTRDTGEFL"
     gene            1282..2235
                     /locus_tag="VFQ79_03250"
     CDS             1282..2235
                     /locus_tag="VFQ79_03250"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HEU0138695.1"
                     /translation="MALRYADSRYESHLSIVDGTAKSPYRYRVLLPYVIEWISQTASK
                     LFELRVRTVTAGLYEVQLFVSLALGLILSFFHFRKYVPDLVALLGCLYLSGLVGFTYL
                     FLSFQPWGWWELPVFTAGLHLILRNRRLLLVLLILVASFLRETAALLVPLYFIARFKY
                     DTRKDLFIWTIILGIVTTAVFIGLRLSLGFAPHVGAELENPLTYRVVHNLTTPLPIIN
                     FLLFYNVLWYFSIKYAKVQPIKIRNCLLFIPLFLIVHALCAHMNETRYYFMIAPIIMP
                     LALTVLSEKYSGERGEHGISPSSYLPIAPEDNSKRQEVILR"
     gene            2250..4349
                     /locus_tag="VFQ79_03255"
     CDS             2250..4349
                     /locus_tag="VFQ79_03255"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEU0138696.1"
                     /translation="MRKILITALILLPLSAQPTFRGFSGSTEKLVEWERRLRSIPEPA
                     RVEAYIRKISSEPHHAGSPASKRMAEYILGLMQEWGLDAKVEVFEALLPYPTSRHLEM
                     VSPARYTARLREPAIPQDGDSSDSNQLPTYNAYSGSGDVTAPVVYVNYGIPEDYAYLD
                     KQGVNVKGKIVIARYGKSWRGTKAKVAQERGAAGCLIYSDPRDDGYFQGDAYPKGAFR
                     PPDGVQRGSVMDMPVSVGDPLSPGWASEPGSRRLRREEATTIMKIPVLPISYRDAQPL
                     LENLEGAVVPEAWRGALPLTYHLGPGPATVRLKVESDWSTRPLYNVIATIPGSEFRDQ
                     WIIYGNHHDAWVNGAQDPSSGAAALLETARALAQLRSQGWQPKRTIKLALWDAEEFGL
                     VGSTEWVEKHLEELKNKAAVYINSDSNGRGEFRAGGSPVLESFVREVIRDIEYPEGGV
                     SVLDRLLSNRQRSGDRDTDFRLGPLGAGSDYVAFVHHAGIASLNLGFSGADSGGIYHS
                     IYDSFDWYKRFGDPDFLFGKALAQLMGTAILRLADADVLPLDPSSLAQAVRRNVNDLT
                     KQTDHKVDLSGVLSALTSLENNGARYEQALRMALENRPPESATLLNLNRTLLGMERAL
                     TTEGGLPGRPWYKHLYSAPGLYTGYGAKTLPGVREAVESKNWEQAQSQAGALAAALSA
                     FDECVRNSIQQLSTTAP"
     gene            complement(4336..4773)
                     /locus_tag="VFQ79_03260"
     CDS             complement(4336..4773)
                     /locus_tag="VFQ79_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138697.1"
                     /translation="MKRFEFRLERVREFRSGQVSLEESKLHALLSEVQALEAARAELD
                     RQRLSEARALVSLGRVEAAELTALETYRMAAVRRAHDLDRRAAVCRQHAENQRVRVLE
                     ARRKMKLLDRLKEKALAKWCIQENAEIENLAAEAYLAKYVRER"
     gene            complement(4776..6098)
                     /locus_tag="VFQ79_03265"
     CDS             complement(4776..6098)
                     /locus_tag="VFQ79_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEU0138698.1"
                     /translation="MTHQSWNVEPYLDRLGRINTLRSSGTVTELTGLLVESDGPAAAI
                     GDFCEVRTSTGNSIRTQVIGFRNGRVLSMPLDETGGLQLGDTVVARADEARVEVGKGL
                     LGRVIDGFGKPLDSGPAIAAEDLYSLYRDAPGPLEREHITEPLVTGIRVIDSLLTCGK
                     GQRIGIFGGSGVGKSTLLGTMARNHSADVSVIALIGERNREVRGFLEHELGPEGLKRS
                     VVVAATSDRPAPLRLRACFVALAVAEYFRDQGASVLLVMDSVTRLAMAQREIGLAAGE
                     PPSQKGYTPSVFNLLPRVCERAGNFSTGSITGFFTVLVEGDDFNEPICDAVRGILDGH
                     IILSRDLGAAGHYPAIDVLNSVSRLASSLSTPEQKAAAQKIREALSAYRRAEDLINLG
                     AYTAGSNPKLDTVIRARERMMDFLRQDSGVHSSRDQTIEQMQSVASLL"
     gene            complement(6095..6757)
                     /locus_tag="VFQ79_03270"
     CDS             complement(6095..6757)
                     /locus_tag="VFQ79_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEU0138699.1"
                     /translation="MKSTSKVLSGDAATALPSIAWQNIGSQQPAGYGVSAGGPVRDED
                     AATYQARIRELEQGAAQKIQQAYREGEAAGAQQAAARVQPLLEKLARTIAEVSGFKAQ
                     FRKEAEQDLVKLAVSIARRVLHRELTVDPEALVGIVRVALDRMDTRDCRRVRVSPADA
                     DAIRAALNRLGLTVNIEVAPDSRLERGAVVFDTSRGQLDGSIETQLQEIERGFTDLVR
                     RS"
     gene            complement(6754..>7407)
                     /gene="fliG"
                     /locus_tag="VFQ79_03275"
     CDS             complement(6754..>7407)
                     /gene="fliG"
                     /locus_tag="VFQ79_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEU0138700.1"
                     /translation="LAKFIHSEHPQTIALILSHLNPSQAAGLLFSLPGEMRADVALRM
                     ANLDQISPEIISKIAAIIGQKLKAIGELSREAYGGVRAVAEMFNRLDSTTSKEILETI
                     EQENPTLVETIRHLMFVFEDLLLIDQAGIKELLSKVDRKLLTIALKGTSDQLRNHFME
                     CMSSRGSEMLREDMEALGPIKIKEVEAAQQQIISIVRQLESEGVINMKGTVGEQYVL"
BASE COUNT         1498 a         2171 c         2060 g         1678 t
ORIGIN      
        1 cgcatcgccg ttcttttttc tagcgctgtt tcccgccttg ttgcagagaa tgcccaagag
       61 cggaggatgg ctcgcgcgcg tgaaggttgt tctgggattt atcattctcg ccgcgatgct
      121 caagtacatc agcaacgtcg atcaggtgct gcaatggaac ctgcttacgc gcgagcgttt
      181 cctgagcgcg tgggtcgtcc tgttcgcact ggcgggcttg tacctgttgg gccttttgcg
      241 aatggagggg gtgcgtccgg aggattcttt gggggtcgga cgtctgttga cggccgcagc
      301 gctgctgatc ttcgcgatca gcctcattcc cggtatattc ggtgcgcggc tgggtgaact
      361 ggacgcgtat gttccgcctc catccgccgg cagcgggctg gttgcaccgg gtgcggcgca
      421 ggatctggca tggatgaaaa atcagtaccg tgaggctctg gatcgcgcca gaagcgagaa
      481 caaaccggtc ttcgtgaatt tcaccggcta tgcctgcacc aattgccatt ggatgaaggc
      541 gaacatgttc acccggccgc agatcgcctc ccgcctgaag gatttcgttc ttgtagaact
      601 ctataccgat gggaccgacg ccgagtccga agcgaatcag aaactacagg aatcccaatt
      661 ctcgacggtt gcaatcccgt attatgctat cctcgacgcc ggtgggaaaa cgattgcctc
      721 gtttgcgggc ctgacacgcg acaccggtga gtttctatag ttcctgaact cggcccctgc
      781 tccaggcaac gccgccggcc tttaaatcct gtggcgtaaa tcgccgaagc gcgtgactcg
      841 aagaatgttt tcaggttgcg gatgcggcga cgcaggacgg acgcacgtac cagaactaaa
      901 tgtactaata aggtctttac atctattatt atctcacgta taattcccta tgctgtctcg
      961 cactggtagc attcatgtgt gacgccatga tcggacgcca ctacaaaggc aaggtctatc
     1021 gcgccgatct cccacgcctt caaccagata ctcgactttg ctttttgaag cttcgatcca
     1081 gggcgctcga tcaaatgagc cgccaagtag ctcttagatg agcccatcga gatggcaccg
     1141 atataaatag gcatcaatcg gaggataggt ccgaatatgc tccgggttga gataaggaaa
     1201 gcacttgcaa ggcccgagtt agtattttca atcttacttt gcgcgatagc tgtttgtctt
     1261 acgttcgatc atttgcactc tgtggcactg cgctatgccg actcgcgcta tgaatctcat
     1321 ctaagcattg ttgacggcac ggctaaatct ccttaccgat accgtgtctt gctcccgtat
     1381 gttatcgaat ggatatcgca gacagcgtcc aagctctttg aattacgtgt ccgcactgtc
     1441 accgccgggc tgtacgaagt tcaactattt gtcagcctcg cgttgggctt aatcctttca
     1501 ttcttccact ttagaaaata tgtgcccgat cttgtggcgc tcctgggttg cctctacctt
     1561 tccggtctcg ttggcttcac ctacctgttt ctctcttttc agccctgggg gtggtgggag
     1621 cttccagttt tcacagccgg gctccatctt atcttgagaa acagacgcct gctgttggtc
     1681 ctgcttatac tggttgcaag ttttctgcgc gaaactgccg ccttattagt tccactctat
     1741 tttattgcta gatttaaata cgataccagg aaagatctat ttatttggac tataattctt
     1801 ggcatagtaa caactgccgt attcatcggt cttcgcttgt ctcttggttt tgccccacac
     1861 gtcggcgcgg agttggagaa tccattgaca tatcgagtgg ttcataatct gacaacaccg
     1921 ttgccaataa tcaatttcct attattttat aatgtattgt ggtattttag tataaaatat
     1981 gcaaaagtcc agccaattaa aatacgtaat tgtttattat ttattccttt atttttgatt
     2041 gttcatgcgc tatgtgcgca tatgaacgag accaggtatt actttatgat tgcgcccatc
     2101 ataatgcccc tggcgctgac agtactatcg gagaagtact ctggtgagcg tggcgagcat
     2161 ggcatatccc cttcgagtta cctgcccatc gcaccggaag acaattcaaa gcgccaagag
     2221 gtgatactta gatgaagcac tgtggacaca tgcgaaagat actgataacg gcactcattc
     2281 ttctacccct ttcggcgcag ccgacgttcc gcgggttttc gggcagcaca gaaaagctgg
     2341 tggagtggga acgccggctc cggagcattc ccgaaccggc gcgcgtggag gcctacatcc
     2401 ggaaaatctc ctcggagccg caccacgccg ggtctcctgc ttctaaacgg atggcggagt
     2461 acatactcgg cctgatgcag gagtggggcc tcgacgcaaa agtcgaagta tttgaagcgc
     2521 tgttgcccta tcccacatcg cgccacctgg agatggtttc accggcgcgc tacaccgcca
     2581 gactacggga gcctgctatt ccgcaggatg gggattcgtc ggattcgaac cagttgccca
     2641 cctacaatgc ttactccggc tccggcgacg ttaccgcgcc cgttgtttat gtgaattacg
     2701 gcattccgga ggattacgca tacctcgata aacagggcgt caacgtgaag ggaaagatcg
     2761 tcatcgcgcg atacggcaag agctggcggg gcacgaaggc caaagtggcc caggaacgcg
     2821 gagcggccgg ctgcctcatt tattcggatc cgcgggacga cggctacttc caaggcgatg
     2881 cgtatccgaa aggcgccttc cggcctcccg atggagtaca gcgcgggagt gtgatggata
     2941 tgccggtctc cgtgggcgat ccgctctcgc cgggttgggc ttcagagccc ggatcgcgaa
     3001 ggctgcgccg cgaggaagca accacgatta tgaagatccc ggtgctgccg atctcctatc
     3061 gcgatgccca gcccttgctt gagaatctcg agggtgctgt ggttccggag gcatggcgcg
     3121 gtgctctgcc cctgacgtat cacctcggcc cggggcctgc aaccgtacgc ctcaaagtgg
     3181 agtcggactg gtcgacacgg cccctataca acgtgatcgc gacgattccc ggaagcgagt
     3241 ttcgagacca gtggatcatc tacggcaatc atcacgacgc ctgggtgaac ggtgcacagg
     3301 atccttcgag cggagcggcc gcactgctcg aaaccgcccg ggcgttggcc cagcttcgca
     3361 gccagggatg gcagcccaaa cgcaccatta agctcgctct ttgggatgcg gaagagttcg
     3421 gccttgtggg ttctaccgaa tgggtggaaa aacacctgga ggagctgaag aacaaggccg
     3481 cagtctacat caattcagac tcgaacggac ggggcgaatt ccgcgctggt ggatcgcctg
     3541 tgctcgaaag ctttgtgcgc gaggtgatcc gcgacattga gtatccggaa ggcggtgtaa
     3601 gcgttctgga ccgtcttctg tcgaatcgtc aacggagtgg ggatcgcgat accgatttcc
     3661 gcctgggccc gctcggagcc ggatccgact atgttgcgtt tgtccatcac gccggcatcg
     3721 caagcttaaa tctcgggttt tccggcgcgg actccggtgg aatctatcac tcgatttacg
     3781 attccttcga ctggtacaag aggttcggcg atccggattt cctgttcggc aaggcgctcg
     3841 cacaactgat gggaacagcc atccttcggc tcgccgatgc cgatgtgttg ccgctcgatc
     3901 cttcttccct ggcgcaagct gtccgccgga acgtaaatga cctcactaaa cagacggacc
     3961 acaaagtgga tttgagcggc gttctctccg cgctaacttc gctcgaaaat aacggagccc
     4021 ggtacgaaca ggcgctccgg atggctctgg agaataggcc tccggaatcc gccacactgc
     4081 tcaatctgaa tcgcacactg ctcggcatgg agcgggcgct cacaacggaa ggcgggctgc
     4141 ccggccggcc atggtacaag catctctatt ctgctcccgg cctctatacc ggatacggtg
     4201 caaagacgct gcccggggtg cgggaggcag tggagtccaa gaactgggag caggcccaat
     4261 cgcaagccgg cgcgctagcc gctgctttga gcgcattcga cgaatgtgtc cggaactcga
     4321 ttcaacagtt gagcactacc gctccctgac gtacttggct aaataggctt cggcggcgag
     4381 attctcgatt tcggcgttct cctggatgca ccatttcgcc agcgcctttt ctttcaacct
     4441 gtcaaggagt ttcatcttgc ggcgcgcttc cagaacacgc acacgctggt tctcggcgtg
     4501 ctgccggcaa acggccgcgc ggcggtccag gtcatgggcg cggcgaactg ccgccatccg
     4561 gtaggtttca agcgccgtca actcggcagc ctccacacga ccgagagaga ccagcgcgcg
     4621 ggcctctgac aaccgctgcc ggtccagttc ggcacgcgcc gcctccaaag cctgtacttc
     4681 ggacaggagc gcgtgcaatt tcgactcttc gaggctgacc tgcccgctcc tgaactcgcg
     4741 cacacgttcc aaccggaatt cgaatcgttt catgctcaga gtagcgatgc gacagattgc
     4801 atctgttcaa tggtctgatc ccgggatgag tgtacgcccg aatcctgccg gagaaaatcc
     4861 atcatacgct cccgggcccg gatcacggtg tcaagcttgg ggttggagcc cgcggtatat
     4921 gctcccagat tgatcagatc ctcggcccgc cggtaagcgg aaagcgcttc ccgaattttc
     4981 tgtgccgccg ctttctgctc gggcgtggag aggcttgaag caagcctgct cacggagttc
     5041 agtacgtcga tggcaggata atgtcccgcc gcgccgaggt ctctcgaaag gatgatatgc
     5101 ccgtcgagga tgccgcgcac ggcatcgcag atcggctcat tgaagtcgtc gccttcgacc
     5161 agcaccgtga agaagccggt gatcgatccc gtggaaaaat tcccagctct ctcacacacc
     5221 cttggcaaaa ggttaaagac ggacggggtg taaccctttt ggctgggcgg ctctcccgcg
     5281 gccagcccga tctcgcgctg cgccatcgcc agccgggtga cagaatccat caccagcaat
     5341 acgctagctc cctgatcgcg gaagtactcc gcgacggcga gtgcaacgaa acaggccctc
     5401 agccgcagtg gcgcgggacg gtcgctggta gcggcaacta caacggaacg cttcaagcct
     5461 tccgggccga gctcgtgctc gaggaacccg cgcacttcgc gatttctctc gccgatcaat
     5521 gcgatgacgc tgacatcggc cgagtgattg cgcgccatgg tgccgaggag ggtgcttttt
     5581 ccgacgccgc tgccgccaaa aattccgatt ctctgccctt ttccacatgt cagcaggcta
     5641 tcgatcacgc gaatgccggt taccagcggc tcggtgatgt gttcccgctc cagcgggcca
     5701 ggcgcatcac ggtagaggga ataaagatcc tccgcagcga tcgccggacc actatccaaa
     5761 ggcttcccga agccgtcgat cacccgcccc aacaggcctt tgcccacctc cacacgcgct
     5821 tcatccgcac gggcgacgac ggtgtctccg agttgaagcc cacccgtctc atcgagtggc
     5881 atcgaaagaa cacggccatt ccggaatccg atcacctgcg tgcgaatgga gttaccggtt
     5941 gaagtccgga cttcacagaa atctccaatc gcggcggcgg gtccgtcgga ttcaaccagc
     6001 aggcccgtca gttcggtaac ggttccgctt gaacgaagcg tgttgatgcg ccccagacga
     6061 tccagataag gctcgacgtt ccaggattga tgggtcatga tcttcgcacc aggtcggtga
     6121 atccgcgttc gatttcctga agctgcgttt caatggagcc atccagttgt ccacgggagg
     6181 tatcgaacac gacggccccc cgctccaggc gggagtcggg tgccacttcg atgtttacag
     6241 tcagtccaag ccggttcaac gcggccctga tcgcgtccgc gtcggcgggg ctgacccgga
     6301 cacggcggca gtcgcgcgta tccatcctgt ccagagctac ccgcacaatc cctacgagcg
     6361 cttcggggtc taccgtcaac tcccggtgca gaacccgccg cgcaatggaa accgccagct
     6421 tcacaaggtc ctgctccgcc tctttccgga attgcgcctt gaagccggaa acctccgcta
     6481 tggtccgcgc caatttctcc agcagcggct ggacccgcgc cgcggcctgc tgcgctcccg
     6541 cggcttcacc ctcacgatat gcctgttgaa tcttttgcgc ggccccctgt tccaactccc
     6601 ggattcgcgc ctgataggta gcggcgtcct cgtctcgaac cggccccccg gcgctcacgc
     6661 cgtaaccggc cggctgctgg gatccgatgt tctgccaggc gatggagggg agcgctgtgg
     6721 cggcatcccc ggaaagcacc ttagatgtcg atttcacagc acgtactgtt cgccgacagt
     6781 tcctttcatg ttgataaccc cttcactttc cagttgccgg acaatcgaaa tgatctgctg
     6841 ttgagcggct tcgacctctt tgatttttat gggaccgagc gcctccatat cttcccgcaa
     6901 catttccgat cctcgtgacg acatgcattc catgaaatga ttccgcagtt gatcacttgt
     6961 acctttgagt gcaatcgtga gcagcttccg gtcgactttg gatagcagct ctttaatacc
     7021 cgcctgatcg atgagcagca gatcttcgaa cacgaacatc aggtgccgga tggtttctac
     7081 cagtgtgggg ttttcctgtt cgatcgtttc caggatttcc ttgctggtcg tggaatccag
     7141 gcggttgaac atctcggcaa ccgcgcgcac gccgccgtaa gcctcgcggc tcaactcgcc
     7201 gatcgccttc agcttctgtc cgataatagc tgcgattttg gagatgatct ccggtgagat
     7261 ctgatccagg ttggccattc tcaaagccac gtcggcccgc atttcaccgg gcaaggagaa
     7321 caacaggccg gccgcctggg atggattgag atgcgaaaga atcagggcta tggtctgcgg
     7381 gtgttcacta tgaatgaatt tggcaag
//
LOCUS       DASTMK010000081         8452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_612481, whole genome shotgun sequence.
ACCESSION   DASTMK010000081 DASTMK010000000
VERSION     DASTMK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612481"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(445..2985)
                     /locus_tag="VFQ79_03280"
     CDS             complement(445..2985)
                     /locus_tag="VFQ79_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0138701.1"
                     /translation="MRIQPGAKLGHYEFEAVIGVGGMGEVYRARDIRLGRYVAVKLLA
                     GHLVQDVSFRARLRQEAAAAARLNHPNVVAVYDAGEDYIVSEFVEGVTLRKLEKPTIR
                     QALEIVAQVASGLAAAHAMGIVHRDIKPENIMITAQGTVKILDFGLAKPVADSVGPAK
                     PGLALTEPGMVMGTVSYMSPEQVRGAQVDHRSDIFSLGLVLHEMLTGKKVFERESAAE
                     IMSAILKEDPPPLPSALPAALAFIVSRCLEKNPESRFQSAHDLGVSLQALATVSQPTA
                     ALPNPSSPRRRKGPFPWVLAVALVFIALAAGWVAGRLLSRGGSVAFRQVTFQRGFVSS
                     ARFSPGSAVIVYSAAWNGEAGDLFLSARGSPEARSIGLRNAHLFSISANNEVAVALNC
                     RYVGDNSQAGTLGVVPLYGGAPRIVMPDVSEADWDPSGKQLALVRSVRGVRRLEYPAG
                     KVLYQTAGWLGDLRFSPRGDRIAFSDHPTRDDDRGRIVTVDLHGNAQELSGEWESIEG
                     LVWTKDASEVWYAASDSGYADTLYASAPGRSPRFVFQFPVSVKLLDLGSDGVLLFTTF
                     DDDRYETWASLPGVSADQSLDWLGSSFPGDLSQDGKLLLFSQYGQAGGRSYGLYLRNL
                     GESAPVRLGEGDAMSLSPDGRYALALQYTEPQRLALFDFQSPEPQLMLPAGLNYQTAA
                     QWFPDSRRIIFEANEAGRPSRIWMQDVPAGKPQPITPEGVSLEGSGLSPDGTKVAARS
                     PEGLLRLYPIGGGGAAEIRGPLPDDRLVRWSPSGQELIVMSSVEGAAQLNQIDLATGK
                     RTPGKRIASLDPVGVTQVTQVRLSADGQAIVYGQNRRVRTLRTAVGLK"
     gene            3419..4579
                     /locus_tag="VFQ79_03285"
                     /pseudo
     CDS             3419..4579
                     /locus_tag="VFQ79_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
     gene            complement(4586..6223)
                     /locus_tag="VFQ79_03290"
     CDS             complement(4586..6223)
                     /locus_tag="VFQ79_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138702.1"
                     /translation="MPIPVKRFPNPLLCLILVCAPLLAASEDTLFRQARENAARFDEM
                     AGAMQRVLKAWLGVADPTTLLLPDRIPGGLRGLKAGDTSRIYTPHNSGADLYPYLILT
                     AHLTDPDLYHGRMMEMLRNEIRYTTRVDSVPGNLPLPPGELGPPSLFGAGEYAKDGLL
                     AVTELLGRTPWYYRMVDMVADAMKRAPVKSKFGNLPAADAELNGDFLQALVRLSTMTG
                     DRRFLEWTRRIADAYIEEVLPGSHGVPSSKWDFDTHRGDGTLRLRDHGNETVVGLTLA
                     YALEQYLDSPRAPRYRQAVGRMLDRVLESANPDGMLYNSVDAATLKPSGPGLSDNWGY
                     VYGAVYTFYQATGEEKYRDAVRHVLKALPKYRNYDWESGSFDGYADSIESAIYLVNRE
                     PVPEALDWVESEIRVMAAMQKPSGFLENWYGEGNFNRTLLLYILYKSQGCRPEHWTAG
                     RGVGAVRDGEKLYLHVQSKGVVKFDFARHRRVLNFDQNYVRLNEFPEWYTVDENTLYS
                     LARRGADQGQIRLGSELIAGIPLEAGDWIIEPGRGTR"
     gene            6321..7898
                     /locus_tag="VFQ79_03295"
     CDS             6321..7898
                     /locus_tag="VFQ79_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0138703.1"
                     /translation="MTILGIGGILHDAAAAILVDGDLAAAVEQNKVARRTRPGHLPDE
                     AISACQRIAGVRDDQIDCVAVVRPIAAGPESAVHLELRSRFPHSQIVVVDHHRAHAAS
                     AYYPSPFENAIVLTLDRSGDFRCGARWIAEGTSLVPDRELYYPDSLGELYTRVTSLLG
                     FEPNAEEHKVQWLSASGDNRMVGVFTDILGLNSTDWPRFDRTYFHGERMTAGGFSSEF
                     YERLGLPDGSEIPQAMRASIAAGVQRATEKAVLAMAGSGENLCLGGGVSTNALLVAAL
                     ENSPQWKNVFVQPAGSNAGTAIGAVFHAWHSIFRQKKRGSLGNFCLGPAFDAEEIKQV
                     LENCKLHFRYLLTIEELIDRAVEEIQDQNIVAWMHGRMEFGPRALGNRSILASPLNPY
                     STENLNLFIKHREPFRKFAASVPAELASEYFEVGTNARFLASVGRVHTAHRKTFESAI
                     MGPGYVRVQTVTQHDNPLYWRLLHAAGKQTGLPVLYNTSFNLFGDPLVCTPRDAVRSF
                     YSSGIDSMFVGHFLLKK"
     gene            complement(7903..>8452)
                     /locus_tag="VFQ79_03300"
     CDS             complement(7903..>8452)
                     /locus_tag="VFQ79_03300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0138704.1"
                     /translation="QAQLTGFNRSSPFGDDYSGQNFVEEVDWTNSHADPILSKLVPPI
                     DGLKPADIHDTYDEIRGAGRRHEAVDMMAPRGTPVRAATSGKIAKLFVSKPGGLTIYQ
                     FDRTEKYCFYYAHLAAYAADLAEGREVQAGDLIGYVGSSGNAQEDSPHLHFAVFELGP
                     DKKWWEGAPINPYKYLLHAAGQ"
BASE COUNT         1752 a         2545 c         2512 g         1643 t
ORIGIN      
        1 tgacaccagg tatgatagcg ccactggcgt ccaccacccg tccgatgatg gaacctctgg
       61 tttcttgtgc cggcgcgatg ccggcaacca tgagacacag catggctgca agcgccaaga
      121 ccgcttttct tgacatgctt cctctcctat ttcgaaaaat cgattgcgct aaccccgtta
      181 ccggcactta cgtgccaaca aattgttcca cttgcatgga acacatttca ataagattaa
      241 agggattata ggtacgaaat aacacgggtg tcaaatcgag cccacaactt cagctccttt
      301 gctctaccct gttttttcgc tgtgctgtaa accgcatccg cctgtcgcgg ctttaacacc
      361 ggcacgtaga aaatgcggat cggtgaaata agtaatcgct ccatacaaaa ggcagatagg
      421 tgatttgtca agtatcttct gcccttactt gaggccgacg gccgttctca aggtgcgcac
      481 tcgacggttc tggccgtaaa cgattgcctg accgtcagcc gaaagccgta cctgcgtcac
      541 ttgtgtgacc ccgacaggat ccaggctcgc gatgcgcttg ccgggcgtcc gtttgcccgt
      601 agcaagatcg atctggttca attgggccgc accctcgaca gacgacatta caatgagttc
      661 ttgcccgctc ggactccaac gtaccagccg gtcatccgga agaggtcccc ggatctccgc
      721 ggcgccaccg ccgccaattg gatacagccg tagcaaacct tcgggagagc gggcagccac
      781 cttggtccca tctggactga gaccacttcc ttccagtgaa acgccctccg gagtgatggg
      841 ctggggcttg cctgccggga cgtcctgcat ccagatgcgc gacggccgcc ctgcctcatt
      901 agcttcgaaa atgatacgtc gcgaatccgg aaaccactga gcggcagttt gatagttcaa
      961 gccagcgggt agcataagct gcggctcagg cgactggaag tcaaacaacg caagcctctg
     1021 cggctcggtg tactgcagcg ctagcgcgta ccgcccgtcg ggtgaaaggg acatggcatc
     1081 gccttcgccc aggcggacgg gcgccgactc accaaggttc cgaagataga ggccgtatga
     1141 gcggccgccc gcctggccat actgcgaaaa cagcagaagc ttgccatctt gcgagaggtc
     1201 gccgggaaaa ctggaaccca gccagtccag cgactgatcg gcggagacgc ccggaaggct
     1261 agcccaggtt tcatatcggt cgtcatcgaa cgtcgtgaag agcagaacgc cgtcagaacc
     1321 cagatccagc agcttgacac tcactgggaa ctgaaacaca aagcgtgggc tccggcccgg
     1381 tgcggatgca tacagggtat cggcgtagcc gctatccgag gcggcgtacc agacttccga
     1441 agcgtccttt gtccaaacca gcccctcaat ggattcccat tcgcccgata gttcctgggc
     1501 attgccgtgc agatcgacag tgacaatccg gccccggtcg tcgtcccggg tcgggtgatc
     1561 ggagaaggcg atccgatcgc cgcggggaga gaaccggagg tcgcccagcc aaccggctgt
     1621 ctggtagagg accttgccgg ccggatactc gagccgacgc actcctctga ccgaccgcac
     1681 cagtgctaat tgtttgccgg aggggtccca gtccgcttcg gagacatccg gcatgacaat
     1741 tcgcggggcg ccgccgtaaa ggggcaccac acccagcgtt ccggcctgac tgttgtcacc
     1801 gacatagcgg cagttcagag ccaccgctac ctcgttattt gccgaaatgg aaaaaaggtg
     1861 cgcattgcgc aaaccgatgc tgcgagcttc gggagagccg cgggcggata gaaagagatc
     1921 tcccgcctca ccgttccaag cggcgctgta gacaatcaca gccgagccgg gactaaaccg
     1981 cgccgacgat acgaagccac gctgaaaagt gacttggcga aacgccacgc ttccgccgcg
     2041 actcagcaag cggccggcga cccagccggc agcaagcgca ataaaaacaa gggctaccgc
     2101 caaaacccaa gggaaaggac ctttccgcct tcgcggcgat gatggattcg gcagcgcggc
     2161 cgtgggttgt gacaccgtag cgagcgcctg caagctgact cccaaatcat gagcggattg
     2221 gaaacgcgat tcggggtttt tctccaggca gcgggatacg atgaacgcaa gcgccgcagg
     2281 taaagccgaa ggcaggggcg ggggatcttc tttcagaatc gcactcatga tctccgcggc
     2341 ggactcacgt tcgaaaacct tttttccggt gagcatctca tgcagcacca ggccaaggct
     2401 gaaaatatcg gagcgatgat ccacctgcgc accccgaacc tgttcgggtg acatatagga
     2461 caccgtgccc atgaccatgc cgggctcggt cagagcaagt cctggcttcg ccggtcccac
     2521 tgagtctgct acaggtttgg caagaccgaa atccaaaatc ttgaccgtcc cctgcgcggt
     2581 gatcatgatg ttctccggct tgatgtcacg gtgcacaatg cccatcgcat gcgcggccgc
     2641 gagtccggaa gcaacctggg ctacaatctc gagggcctga cgaatcgttg gcttctcaag
     2701 cttgcggaga gtgacgccct ccacaaattc ggagacgatg taatcctcac cggcatcgta
     2761 gactgccact acattgggat ggttcaagcg agcggcggct gcggcttcct gtcgcagtcg
     2821 cgcccgaaag gagacgtctt gcacaaggtg tcccgcgagc agcttaacag cgacatagcg
     2881 gcccagccgg atgtccctgg cccggtagac ttcgcccatc cctccaacac cgattacggc
     2941 ctcaaattca tagtggccaa gcttcgcgcc cggctgaatg cgcatcggat catcctctcg
     3001 tcgggcagga tgtggcgctg cggccacgcc gagaatcgcg ggtgcaggcg cagcccggat
     3061 gcagtctgaa ggccttacca ttatcggccg aattcatccg aggcgccacg gcgcgagaag
     3121 agaagtgcaa gggcgagccg cgcaaccgga tggcatgtgc cagtcctcca tgctatttcg
     3181 cgacaattgg cgcaggccaa gaatagaatc aggtattgcg gggaggatgt ctatgaaatg
     3241 ccacgctctt cttattgctg ttttggccgc tgcaactctc tgcggggccg atttcaaagc
     3301 tggattcggg cggcgccaga ttaccccgcc gatgccgata tggctggctg gtttcgcggc
     3361 tcgcacgcac ccggctgaaa gcatcgccca cgatctgtga acgaaggcgc ttgcactcga
     3421 agataacagt ggccggaagc tgatcgttat caccgtggat ttggtaacaa tgccgaaggc
     3481 gatgacggat atggtagctg ccagggtgat gcgccggcac gccattgacc ggtcacacct
     3541 gctgattaac ttttcccata cccactcggg ccccgcgttg agctggccct cgaactcaga
     3601 ccgcacgatg ataggccgca ttgagggtta ccggaataag gtcatggact ccatggcgga
     3661 ggcggctgcc gctgccgtca gcgaccttca gcccgcgcag gtctcgtacg gcgccggacc
     3721 ggtaggattt tcccataacc gtcgtgaacg cgttcccggc ggaggctggc gcttcggaat
     3781 ggaccctgat gggcccgtgg accggaccgt tcccgtgttg cgggtatcca gcgccgatgg
     3841 caagctgcgc ggtgtgctgt tcggtctttc ctgtcatgct tccgcgctga cgccggcgtt
     3901 ctacgtggtc agcggcgact accctggcat cgctcaggcc gcgctggaaa agacccatcc
     3961 cggtgtcacg gccctgttca tgcaactcgc cggcggagat cagaccacct acccgcgggg
     4021 caagatggaa ctggccgaga agtacggtga ggaactggcc ggcgaagtcg accgcatcgt
     4081 ggcagctcga atgaaacccg tgcgcggtcc gctcaagagc gccatgctca ccaccgaact
     4141 acccttcgcg ccgttttcat tggagaaatt cgagcagcag gcaaaggagg atgatgcact
     4201 gctccgcaac cacgccgaac ggatgctgaa gctgtatcaa tcggggcagc cgccgctgcc
     4261 acggctgccg tacactctgc aggccattca gttcggaaag gacctgaccc tgctggccat
     4321 gaacggggaa gtggtggccg agtacagtct gcgcgtgaag agggaatacg gcgcggaagg
     4381 gatgattgtg gctgggtatt ccaacgaaat acaatgctac atcccatcgg cgcgaatcct
     4441 taaagaagga ggctacgaag cccgcgatgc aatcctgatg gggtcgctcc ctggtcctct
     4501 gggcgaggag gtggaggaaa cgatcttcgc ggctattcga gagctgatgc aaacgattgg
     4561 gaaggctcca cagcggtagc cgggcttatc tcgtgccgcg tcccggctca atgatccagt
     4621 cgccggcttc gagcgggatg cccgcaatca gttccgaccc gaggcggatc tgtccttgat
     4681 ctgctcctcg ccgggcaagg ctgtacagcg tgttttcatc gacggtgtac cactccggaa
     4741 attcgttcag gcgaacatag ttttggtcga aattgagtac tcgcctgtgc cgcgcgaagt
     4801 cgaacttcac aacgcccttg gactgcacat gcagatacaa cttctcaccg tcgcggacgg
     4861 cgcccacgcc gcgcccggcg gtccagtgct ccggacggca gccctggctt ttataaagaa
     4921 tgtagagcag cagggtgcgg ttgaagttcc cctcgccgta ccagttttcg agaaagccgg
     4981 agggcttctg catggcggcc atcacccgga tttccgactc cacccaatcc aatgcttcgg
     5041 gaaccggctc tcggttcaca aggtaaatgg cgctttcaat cgaatcggca taaccatcga
     5101 acgatccgct ctcccaatcg taattgcggt acttgggcag cgctttcaaa acgtggcgca
     5161 cggcatcgcg gtacttctct tcgcccgtcg cctggtagaa cgtgtacacc gcgccataga
     5221 cgtagcccca gttgtcggag agtcccgggc cggagggctt caaagtcgcc gcatccacgg
     5281 agttgtacag catgccatcc ggattcgcgg attcgagcac gcggtccagc atgcgtccga
     5341 ccgcctggcg gtaacgtgga gcgcgcggtg aatccagata ctgttccagg gcgtaggcca
     5401 gcgtcaaccc cacgacagtt tcattgccgt gatcgcggag gcgcagcgtc ccatcgcccc
     5461 gatgggtgtc gaagtcccac ttggaggaag gaacaccgtg agaccccggc agtacttctt
     5521 cgatataggc gtcggcgatg cggcgggtcc attcgagaaa gcgcctgtcg ccggtcatgg
     5581 tagacagccg taccagcgcc tggaggaaat cgccattcaa ttcggcgtcg gccgcgggca
     5641 ggttgccgaa tttcgatttc accggcgcgc gcttcatcgc atccgccacc atatccacca
     5701 tgcggtagta ccagggcgtg cgcccaagca gttcggtcac cgccagtaag ccatccttgg
     5761 catactcgcc cgcgccaaag aggctgggcg gtccgagttc gccaggggga agcggcagat
     5821 ttcccggaac ggaatcgacg cgcgtcgtgt agcgaatctc gttccggagc atctccatca
     5881 tccgcccgtg atacagatcc gggtcggtga ggtgcgcggt gagaatcaga tacgggtaca
     5941 ggtccgcacc ggaattatga ggcgtataga tgcgcgacgt gtcgccggct ttcaggccgc
     6001 gtaagcctcc cggaatgcgg tccggtagaa gcagcgttgt gggatcggcc acacccagcc
     6061 aggctttcaa aactcgttgc atggcgccgg ccatttcatc gaagcgagcg gcattctccc
     6121 gcgcctgccg gaagagcgtg tcttcggacg cagccagcag cggcgcgcat accaggatga
     6181 ggcagagaag aggattcggg aagcgcttga ccggtatcgg catgatctcc ctattattaa
     6241 gcccggcagg gccgtgcgct acactgatca attgatgctt cgagtcgctt cgccgtctgg
     6301 cgaaggcatg aactaaatca atgaccatac ttgggattgg cggcatactg catgatgccg
     6361 ccgccgctat cctcgtggac ggtgacctcg ccgccgccgt cgaacagaac aaagttgcgc
     6421 gccggacccg gccggggcac ctgcccgacg aggcaatctc ggcttgccag cggatcgccg
     6481 gcgttcgcga cgatcaaatc gattgtgtcg cggtggtgcg gcctatcgcg gccggtccgg
     6541 aatcggccgt ccatctggaa ctgcgatcgc gctttccgca cagccagatc gtggtagtgg
     6601 atcatcaccg ggcacacgcc gcttcggcct attatccgtc gccattcgaa aatgcgatcg
     6661 tgctgacgct cgatcgctcg ggcgattttc gctgcggtgc gcggtggatc gccgagggta
     6721 ccagccttgt tccggaccgc gaactgtatt acccggattc tcttggcgag ctttatactc
     6781 gggtcaccag cctgctcggc tttgaaccca acgcggaaga gcataaagtg cagtggctgt
     6841 ccgcttccgg tgacaaccgc atggtgggcg tcttcacgga cattctgggc ttgaactcca
     6901 cggattggcc ccgcttcgat cgcacttact tccatggcga acgaatgacg gcgggcggat
     6961 tcagttcgga gttttacgaa cgcctcggcc tcccggacgg gagcgagatt ccacaggcta
     7021 tgcgggcctc gattgcggcc ggtgtgcagc gggccacgga aaaggctgta ttagcgatgg
     7081 ccggcagcgg cgagaatttg tgcctgggag gcggagtgag cacgaacgcg ctgctcgtcg
     7141 ccgcgctcga gaattcgccg caatggaaga acgtttttgt gcagccggcg ggcagcaacg
     7201 cgggaacggc catcggcgcc gtgttccacg cctggcacag catattccgg cagaagaaga
     7261 gaggatcgtt agggaacttc tgtctcggac cggcatttga cgccgaagag atcaaacagg
     7321 tgctggagaa ttgcaagctg catttccgct acctgttgac cattgaagaa ctcatcgatc
     7381 gtgcggtaga agaaatccag gatcaaaaca tcgtggcctg gatgcatgga cgaatggaat
     7441 tcggcccgcg cgcgctgggc aaccggagca tcctcgcatc accgctgaat ccatactcca
     7501 cggaaaatct caacctgttt atcaagcacc gtgagccgtt ccggaagttt gcggcttcgg
     7561 tacctgccga attggcgtcc gagtatttcg aagtgggcac taatgcccgc tttctcgcct
     7621 cggtcggccg cgtccacacc gctcatagga aaaccttcga gtccgccatt atgggccccg
     7681 gatacgtaag ggttcagaca gtgacacagc acgacaatcc actgtactgg cggctgctgc
     7741 acgcagcggg aaaacagacc gggttaccgg tgctctataa cacgtctttc aacctgttcg
     7801 gcgatccgct ggtctgcacg ccccgcgacg cagttcgcag cttctattcg tcgggaatcg
     7861 attccatgtt cgtaggccac ttcctgctga aaaagtagga ggctactgtc cggctgcgtg
     7921 cagcagatac ttgtaaggat tgatgggggc gccttcccac catttcttat cggggcccag
     7981 ctcaaaaacg gcaaagtgaa gatgagggct gtcttcctgc gcattgccgg aggacccgac
     8041 gtatccgatc agatcgcccg cctgcacctc ccggccttcc gcaagatcgg cggcataagc
     8101 cgccaggtgt gcatagtaga agcaatattt ctccgtgcga tcgaactgat aaatcgtcag
     8161 gcctcccggc ttgctgacaa acagtttcgc gatcttcccg gatgttgcgg cgcgtacggg
     8221 tgtaccacgc ggcgccatca tgtccactgc ctcgtggcgc ctcccggcgc cccgaatctc
     8281 gtcataggta tcgtggatgt ccgccggttt caatccatca atcggtggaa cgagtttgct
     8341 caggatggga tcggcgtggg aatttgtcca gtctacttct tccacaaagt tctgcccgga
     8401 gtagtcgtct ccgaacgggc tggaacgatt gaaaccggtt agctgcgcct ga
//
LOCUS       DASTMK010000082        16708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_580253, whole genome shotgun sequence.
ACCESSION   DASTMK010000082 DASTMK010000000
VERSION     DASTMK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16708
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580253"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFQ79_03305"
     CDS             <1..755
                     /locus_tag="VFQ79_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138705.1"
                     /translation="PKATSAWENLGRTAHWLREHIGLFRQPAIPIVTALVEPGAATAE
                     IANLLYRRNVSPFLASASDPPAPDARATLAVVAANLRPPSPEITGRILAHAEAGSTVV
                     AAGLPSRAWWQRPELKPVRSEPDREFYDFGKGQVVAYKRSIVDPSEFALDVIDIISHK
                     QRAVRLWNASSVIALATASPRKGERLLPLINYGAPIDTELQARVQGHFSKAQLLRPDA
                     QPLTLPVVKRGSTTEVFVPELKRAGVVVFGGG"
     gene            845..2086
                     /locus_tag="VFQ79_03310"
     CDS             845..2086
                     /locus_tag="VFQ79_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138706.1"
                     /translation="MATAGAAAGMEGLGAPARMPQSHFGLHPFIERNPKAVFIRRTKV
                     PHKMHEESKLREGLALAREIFVPLQQQGIPVSHRIVLKPNVCSVRSRNRPHVENWGTG
                     TDPQFYEGMVMGLKELGLKKFHFVEANNFPAWNFRGFVDINERQGIEMNEPERRVRNF
                     RDGYEMTWSKVPDGVVYTSIPHYAPVNEPDTWLLNIAKWKAHGMCLTQSVKNEQGLVV
                     LPYVRFCPGWKMVTGVPDFMKPNIHPRVEPVINKLFESHKRMNYARYDSAATLSPMHQ
                     EIWAHKTCDNQSVLKTGLAMIEGIYGRDGNGFGIGNDYLTNLVMFSKDKFRLDIIGLY
                     LGGHEPGNINLYRIAKERGLSDTFNPWEIPVYEWIDGEAVPRKLTDFERTPLKTYYLQ
                     KQGEPEYHLVNEPFDYDRYKV"
     gene            complement(2097..2867)
                     /locus_tag="VFQ79_03315"
     CDS             complement(2097..2867)
                     /locus_tag="VFQ79_03315"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU0138707.1"
                     /translation="MPIENLIDPKVQPERIATGFRFTEGPVFSRRGYLLFTDIPNNRI
                     LKWEEGELTTFRENSNGANGLTFDHQGRLLVCEVGKVTRTEKDGSVTVLASEGIERAN
                     DLVYAIDGSIYFTDPAKSRIYQIQRGGKARVAAEDCTGPNGVALSPNQQKLYAADSKQ
                     RVLRVYDIAPDGALSKGRVFAECRGDGLKTDEAGNVWVASEGGLWVFDPSGTRLGVVS
                     TPEQPSNCNWGSGFRGLYITARTSVYFIRTKVPGTRTY"
     gene            complement(2868..4070)
                     /locus_tag="VFQ79_03320"
     CDS             complement(2868..4070)
                     /locus_tag="VFQ79_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138708.1"
                     /translation="MRRREFLGTALAAAAPVQGASRKKIAALSTTYHVRSHSDNFITR
                     FLEGYWIGEKYYEPPHPIVSLYMDQIHPADIGRRLSTAYGFPVKPSIRESLTLGTGKL
                     AVDGVLLVAEHGNYPHNEKNQHLYPRFEFFEQVVQVFRESGRSCPVFVDKHLSYDWKK
                     ARRMYDWSRELKFPLMAGSSVSVTFRRPELDPPLGAPMETALSVGSGWVGDGGIFHNL
                     EALQAFVERRQGGEVGVRAIQHLAGDAVWKAAEDGAWSKQLMYAALKRGLKVRPGRPE
                     EVKDPVLCLVEYRDGFRAGVIMLPGLVSEYLVAVKLKNRPEPDAALCYIPIENSNNFS
                     PLVHAISHMFETGERLIPVERTLLTTGALAALMESAYQGHKKMETPELSIAYKASRQS
                     YFAHGVGS"
     gene            complement(4067..5242)
                     /locus_tag="VFQ79_03325"
     CDS             complement(4067..5242)
                     /locus_tag="VFQ79_03325"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU0138709.1"
                     /translation="MPLSFILGLLLLQAGNLQERFEQLMRDILIFDAHIDTPRYTVDE
                     GYRWAEEHSYYETDIPRMKRGRVGAILFGVYVEPQEYPQQLWLPRALQCIDAFHEEAR
                     RNAADVEVAYTAGDIVRIRKEGKIAVMVSLEGGHLILDSLPLLRLYHRLGVRYMTLAH
                     FRTNNWADAATDVPVHNGLSAFGRDVVREMNRLGMMVDVSHISDKAVLDAVEVSRSPV
                     IASHSSVKALCDISRNMNDDIIRAIARRGGVVCINFHAGYLDKQAYDVYIGNRPERVR
                     EVKEALALHSSNPKRWELARAVLQRYYKLMPPVEVKQLLRHIDHVAKVAGVDHVGLGS
                     DFDGISGMVPRGLEDVSKYPVLVRGLLEMGYSDNDIRKIMGENLLRVMRANEEGAGK"
     gene            5511..7484
                     /locus_tag="VFQ79_03330"
     CDS             5511..7484
                     /locus_tag="VFQ79_03330"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0138710.1"
                     /translation="MRILVPLFLVSISFSAFAQQPRTPAPNKEFEALQAHFERVIGRR
                     FDQIFRGITTVEQWEQRKPKLRATLEKMLWHDFRWPDTPPPATITYKEDRPGYTLENV
                     VIETAPKLFLTANLYLPRNGRKPFPVVLYQCGHANKSYYARHGAWFAANGIATLVMDN
                     IEMGEVEFTHHGVYSNAWFHWYSRGFSPLAVELLNARRAVDYLASRPDLDRNRIGATG
                     RSGGGMTTFFLAALDERIKASAPVSGTLSTTGWVRQRLTAAHCDCQYPVNSHGMLYSE
                     VGALIAPRAQLQCNADSDRGFPMDAFNEMVEKTREVYHLYKADGALRTAIAPGGHADT
                     EAIRLPVYSFFLKEFLGKETPVAAEGPIDIPPAEQLVCHRNGLPLDEKLTRIDEELIP
                     PRTAFQQTLTGKAREQRRKELTGALREEVFRYFPEQPAALEPQWEAETTIQGRTIRKV
                     SFTSFEGLRVKGVFSVPAGSTPAGKLPGLLVVDHRKGIPVWGNEQPLERNQWGRRAVL
                     IVETLDTGSRVLEQNLRSFSDNDLLHHMKRQAMVAGTTLESMQVYEILRSLELLRTLP
                     QVDSANVTITGKAEAGVNGMYAALLDGKVQQVVLHSPTASHIQGPHYLGVLRYTDIPE
                     VAALLAGSLRVYGEVPQALRASLRCASLADCLR"
     gene            7534..8787
                     /locus_tag="VFQ79_03335"
     CDS             7534..8787
                     /locus_tag="VFQ79_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU0138711.1"
                     /translation="MELHQALLGAGEALLIGFLIGAQREASRGESEKQPGIRDFTLIA
                     LVGALSALLGSDALAVCALVALAVLLAVYHYQVKERSGVTTEVAAVTTFFLGYLTATP
                     RLQHGTLVAVSLTIAVLFLLDAKVTLHNFVRRTITETEFRDTIRFLALVFIIYPVLPE
                     EPMGPYGFFHARKVWLFVILVCSISYFGYFLRKFAGERAGANLAGVLGGIASSTAATV
                     AFARMSGTASDESGHEEGAAIISNTLQFPRILVLLYIINHQVAQACVWPLLAMTAAGG
                     IFSLVRAHGRNGRVTPPTFINNPFSLGPALTFGALLTAVLFLSRAALEAFGTGSIQWV
                     GLLSGLADADAVLLAVAELYEQAGISIGVASGTVLLALAANAITKSVIAVIAGTRRFA
                     RGLLVAFAGIFTAGGVAWLVQSALQ"
     gene            complement(8790..9647)
                     /locus_tag="VFQ79_03340"
     CDS             complement(8790..9647)
                     /locus_tag="VFQ79_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0138712.1"
                     /translation="MNLKHILIPVDFSLPVLAAAADARKLASQPGTRITLLHVGQGNG
                     VTVGPASKEGWIAGATGGDAVRVVQGLESILTGLSVQQHVLQGDPAEKIVEYAREAGV
                     DVIIMPTRGVGTVRRFLLGSVTAKVLHDVDCPVWTVVHDMKIEDDATSPAVRRVACAI
                     DLGPESERILRWASDFAGQFEAALTVIHASPQLEPILGVVHDPEWRASVGAVLRERIG
                     KLNTDTGVSAEVKLVGGEPGKAVSAAAGELKADVLVIGRPAANRFLGRLRTNAYAIIQ
                     HSPCAVVSV"
     gene            complement(9666..10538)
                     /locus_tag="VFQ79_03345"
     CDS             complement(9666..10538)
                     /locus_tag="VFQ79_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0138713.1"
                     /translation="MTEFKHILFPVDFSAACIEAAPHVLKMARLFNSRLTALHVLNIV
                     RSGYTDLDASLVWLPEEIAEMKSSGQEHLNHFVREHLNYETVSSVVEDGEPASVITSF
                     VEREKVELVMLPTRGTGRFRRYMLGSVTAKVLYDCPCPVWTSAHTEQDGEQKAPASPV
                     LLCATNLEPASAELLRWASQLAAGLSAVLQVIHVVPAVEEESRNPGAIAVRDYLFEKA
                     REKWNRVREEAGIDVDLCLAGGDIARRVAAEAARHKACATAIARGRLNKPFGGLRTQA
                     YSIVRESPCPVIAM"
     gene            complement(10697..10942)
                     /locus_tag="VFQ79_03350"
     CDS             complement(10697..10942)
                     /locus_tag="VFQ79_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138714.1"
                     /translation="MSTNLLDKRQVESSVEQAMPPPAVKTAGTDEPGSPLFRGLADLR
                     AEYRSENRRERMLQIGGALIGTAILFAVLYVSMMWME"
     gene            complement(11010..12497)
                     /locus_tag="VFQ79_03355"
     CDS             complement(11010..12497)
                     /locus_tag="VFQ79_03355"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEU0138715.1"
                     /translation="MYKVDFDNRIVITGFGAVAQSVLPILLKHLRISPRNITVVDFNY
                     RGQAFEPWQARGVRFIQERVTPDNLHRLLSSNVGRGGLIIDLAWSIDFFDIAQWAHDN
                     EVLYVNASLESWDATPDLRGASLFDRSLYRRYVRVLEMAGRWSNGTTAVIDQGANPGL
                     ISAFVKKGLLDIASRAVRENALSDAQRYELEWLLERRDFARLARELGVKVIHCSERDT
                     QRSAHPKSAGEFVSTWSVEGMWEECVAPCELGWGTHEKSLPANALVPDIGPANQVILP
                     RFGMDTFIRSWVPEREILGMAIAHGECFSLSHALTVRDATGRLYRPTVAYAYVPCPAS
                     LQSLEELRSRNYELHPVRRILTEDIMEGTDTMGALIMGHRYQSWWTGSSLSIERARQS
                     VPDGNATTLQVAAGIVPAVLWAMQNPRKGLRFSEDLPHEEILRTAAPYIGSIRSDAVA
                     WRPAPESNDPWQFGHFVHSLGAEVAQPAGVFSAAAGGQYSALARG"
     gene            complement(12810..14351)
                     /locus_tag="VFQ79_03360"
     CDS             complement(12810..14351)
                     /locus_tag="VFQ79_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU0138716.1"
                     /translation="MFPGERATGNIFISYRRADSAAYAGRICDHLSAVFGADRVFMDV
                     DDVAPGQEFAKVIGNTIAACDVVLVVIGPGWLEMLRERSDVQQQDYVRYEIEAALARG
                     IAIVPVLVGGAGMPQAAGLPDRLAPLALYQAAELRDSTFREDCMRLANSLGVQPGPDN
                     SRVKHQTRRGKTLVFGGVAALFVLIVLAGWMAIGPRSEGRARRAEINALLATARVQAD
                     RTEYESAFHTCRNLLKIDPSNPGALDLQADMAMRWLQNFSVVVNEGSKAEDIAGPRLA
                     EILLVLDAALARTKLQGQRAADVLAHLGWAHWLNQHLAYKEFGTAAERDLRHALKIDP
                     SNVFAHAMLGNWIIQTNGDVSEALHHFDLAIQTNKERPLVRRLQLGAMAYNSHPDLRS
                     ALIRVADEMRRNGESLSDRDRRRILTAYSPTVNTVEELNQSLAAVPPKNAWETYLWLD
                     DGQNQGNERQTRREFIHASILEIGGQRADALAAFEKLRQDLKDRGYDGRIVRHVSEAV
                     KRLSK"
     gene            complement(14374..15321)
                     /locus_tag="VFQ79_03365"
     CDS             complement(14374..15321)
                     /locus_tag="VFQ79_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0138717.1"
                     /translation="MQPGIESSLKIREMTDRDRSEVSELIYISINHWYQMHGRPPIFH
                     GDPQATEVFYDVYAMLDPGCAVVAENTRTGRLMASCFFHPRERHVSLGIMNVHPNYFG
                     QGAGKALLRFIIDYTEQHGFPALRLTQSALNLDSFSLYNTAGFVPRCTFQDMFLTVPK
                     TGMKFRVAELDRVREATLADVPAMISLELEVSGIMRERDYCYCIENHQGIWHVSVYES
                     NDGGIGGFMISCVHPAMNMLGPCVARGEQEAAALILRELDHYKGRTPLFLLPVEKAAL
                     VRLAYDWGARNCELHLCQVRGEFQPFRGISMPSFLPESG"
     gene            15427..16356
                     /locus_tag="VFQ79_03370"
     CDS             15427..16356
                     /locus_tag="VFQ79_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0138718.1"
                     /translation="MSTQISEQVSSGVSRRRFLGSAAATGTLALARIPVLSGAPENPA
                     GFQIGCYTRPWDQFDYKVALDGMAEAGYKYAGLMTHKGKSWVIVTTDTTPEEAASMGE
                     EVRRRGMELSSIYVGNFAVEKSIEAGVAGLKRLIDNCAAARSPRMLVSGTGKDALVEA
                     YYKVVAECCDYAASNKISISVKPHGGSNATAAQCRKIVEMVGKKNFGMWYDPGNIFFY
                     SEGKIDPVEDVPDAHGLIMGMSIKDYKAPKEVMVTPGTGLVNFREVLKRARQGGFTRG
                     PLIVECLDRGDSAAKITAEARKARQLLEELTSA"
     gene            complement(16374..>16708)
                     /locus_tag="VFQ79_03375"
     CDS             complement(16374..>16708)
                     /locus_tag="VFQ79_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138719.1"
                     /translation="MAAPPDRSRRSGSDDSVLWYGIDRTQRQKAIPAEQPKRQLSDVR
                     EGWKAFHQLRVQMLEYARTTQDDLRSHVVQRERCDAYQWFLLISTHAQRHTLQIREIK
                     THPGFPDR"
BASE COUNT         3424 a         5055 c         4800 g         3429 t
ORIGIN      
        1 atcccaaagc aacatcggcg tgggagaacc tgggacgaac ggcgcactgg ctgcgcgagc
       61 acatcggcct gttccgccaa ccggccatcc cgatcgtgac ggcgctggtg gagccgggcg
      121 cagctacggc ggaaatcgcc aatctgctgt atcgccgcaa tgtttccccc tttctcgcgt
      181 ccgcttcaga tcctccggcg ccggatgctc gtgcaacgct ggcagtggtc gccgcgaact
      241 tgcgtccgcc ctcaccagag ataaccggcc ggattcttgc ccatgcggaa gccgggagta
      301 cagttgttgc cgccggcctg ccttcgcggg cgtggtggca gcggccggag ttgaagccgg
      361 tgcgtagcga gcccgaccgg gagttctacg attttggcaa agggcaggtg gtcgcctata
      421 aacgctccat tgtggacccc agtgaatttg ccctcgacgt gattgacatc atcagtcaca
      481 aacagcgtgc ggtgcggctt tggaacgcgt catctgtaat tgcgctggca accgcatccc
      541 cgcgaaaggg cgagcggttg ctgccgctca tcaattacgg tgcgccgatc gatacggagc
      601 tgcaggcgcg tgtgcaaggc catttttcga aagcccaatt gctgcgcccc gacgcgcagc
      661 ccttaactct tccggtagtg aaaaggggaa gcacaacgga agtattcgtg cccgaactga
      721 aaagagccgg ggtggttgtg ttcggcgggg gctgatttcg attaaactgg atgacaacta
      781 tcgtggttga ccggagatgg agggaaggat gttgaatcgc cgtgatttta tccgagcagg
      841 tgcaatggcg accgccggag cggccgccgg tatggaaggg ttgggagcgc ctgcacgcat
      901 gccgcagtcg cacttcggcc ttcatccgtt tattgagcgg aatcccaagg ctgtattcat
      961 acgccgcaca aaagttcccc acaagatgca cgaggaatca aagctccgtg aagggctggc
     1021 cctggcgcgc gagattttcg ttcccctgca gcagcagggc attcctgtca gccatcgcat
     1081 cgttctgaag ccgaacgtct gtagcgtgcg cagcagaaac cggcctcacg tggagaactg
     1141 ggggacgggc accgatcccc agttttatga aggcatggtc atgggtctca aggaactggg
     1201 gctcaaaaag tttcacttcg ttgaagcgaa taattttccc gcctggaact tccgtggttt
     1261 cgtcgatatc aacgagcggc agggcatcga gatgaacgaa ccggagcggc gcgtgcggaa
     1321 tttccgcgat ggttacgaga tgacctggag caaggtgccc gatggcgtcg tctatacgag
     1381 catcccgcat tatgccccgg tgaacgagcc ggatacctgg ttgctgaata tcgccaagtg
     1441 gaaagcgcac ggcatgtgcc tgacgcaatc cgtgaagaac gagcagggcc tggtcgtgct
     1501 tccctacgtc cggttttgcc ccggctggaa aatggtgacc ggtgtaccgg atttcatgaa
     1561 gcccaatatt catccgcgtg tcgagccggt catcaacaag ctatttgaaa gccataagcg
     1621 catgaactac gcgcgttacg acagcgccgc gacgctgagt cccatgcacc aggagatctg
     1681 ggcgcacaag acgtgcgaca accagagcgt attaaagacc gggctcgcga tgattgaggg
     1741 tatctacggg cgtgacggaa acggattcgg aatcggcaac gactatctga cgaacctggt
     1801 gatgttcagt aaagacaagt tccggctgga catcatcggt ctctacctgg gtgggcatga
     1861 acccggcaac atcaatctct accgcatcgc caaagaacgc ggcctcagcg atacgttcaa
     1921 tccctgggag attccggtct acgaatggat tgatggcgag gccgttccgc gaaagctcac
     1981 ggatttcgag agaacaccgc tgaagacgta ttacttacag aaacaaggcg agccggagta
     2041 tcacctggta aacgagccgt tcgattacga ccggtataag gtgtaaggcg acgcgtttaa
     2101 tacgtgcgtg ttcctggaac cttcgtgcga ataaaataaa ccgacgtccg ggctgtaata
     2161 tatagcccac gaaagccgct accccaattg cagttactcg gctgctccgg tgtgctcacg
     2221 acgccgagac gcgtgccgga tggatcgaag acccacagac cgccttccga cgcaacccat
     2281 acgttacccg cctcgtctgt tttgagcccg tcgccgcggc actcggcaaa aacgcgtcct
     2341 ttactgaggg cgccgtcggg tgcgatgtca tagacgcgca gcacgcgttg cttggaatcc
     2401 gccgcgtaga gtttctgctg gttcgggctc aacgccaccc cgttcggccc ggtgcaatcc
     2461 tcggcggcga cccgcgcttt tccgccacgc tggatctggt aaatgcggga tttcgccgga
     2521 tcggtgaagt agatactgcc gtcgatggcg taaaccaggt cgtttgcccg ttcgatgccc
     2581 tcggacgcca gcacggtaac gctgccgtct ttttcggtac gggtcacctt gcccacttcg
     2641 cataccagca ggcgtccctg gtgatcgaac gtaagtccgt tggcgccgtt actgttctcg
     2701 cggaaggtgg taagttcacc ttcttcccat ttcagaatcc ggttgttcgg aatatcggta
     2761 aacagcaggt atcccctccg gctgaacacc ggcccttccg taaaccggaa ccccgtggcg
     2821 atacgctccg gctgcacctt tggatctatc aggttttcga tcggcattta cgaacctact
     2881 ccatgagcaa agtaagactg ccgggacgct ttgtaagcaa tggacagctc gggtgtttcc
     2941 atcttcttgt gaccttgata tgcggattcc atcaaggccg ccagggcgcc cgtggtaagt
     3001 agtgtccgct cgacaggaat aagccgctcg cccgtttcaa acatatggga gatagcgtgt
     3061 acaagcgggc tgaagttatt cgaattttcg attggaatgt aacagagcgc cgcatccggc
     3121 tcaggccggt ttttcagctt gacggcgaca agatactcgc tcaccagacc gggaagcatg
     3181 atgacgcccg ctcgaaatcc atcccggtac tccaccaggc acaagaccgg gtccttcact
     3241 tcctctggcc gtccgggcct caccttgaga ccgcgtttca gcgcggcata cattagctgc
     3301 ttcgaccacg ccccgtcctc cgcggctttc cagaccgcat ctccggcaag atgctggatg
     3361 gcgcgcacgc ccacctcgcc gccctggcgc cgttccacga atgcctggag cgcttcgaga
     3421 ttgtgaaaga tgccgccgtc accaacccaa ccggatccga ccgaaagcgc ggtttccatg
     3481 ggcgctccca gtggcgggtc cagttccggc cggcggaacg tcacggacac cgacgagccg
     3541 gccatcaggg ggaacttcaa ttcgcgggac cagtcgtaca tgcgccgggc cttcttccag
     3601 tcgtaggaga ggtgcttgtc gacaaagacc gggcaactgc ggccggattc acgaaatacc
     3661 tggaccacct gctcgaagaa ttcaaagcgt ggatagaggt gctggttctt ctcgttgtgc
     3721 ggatagtttc cgtgctccgc gaccagtagc accccatcga cggcaagctt gccggtgccg
     3781 agcgtgagag actcccgtat cgagggtttg acagggaagc cgtacgcggt cgagagccgg
     3841 cgcccgatat cggccggatg gatctggtcc atataaagcg acacaatcgg atgcggcggc
     3901 tcgtagtact tttcgccgat ccaataccct tcgagaaaac gcgtaatgaa attatccgaa
     3961 tgcgagcgga cgtgataagt cgtggaaagc gcggcgattt ttttccgcga ggctccctgt
     4021 accggcgctg ccgcggcaag cgccgttccc aagaattcgc ggcgcctcac ttccctgccc
     4081 cctcttcgtt cgcccgcatc acacgaagca ggttttcacc catgattttg cgaatgtcgt
     4141 tatctgaata acccatttcg agaagccccc tcactaagac cggatacttt gaaacgtctt
     4201 ccaggccgcg cgggaccata ccggagatgc cgtcgaaatc ggacccgagt ccgacgtgat
     4261 ccacacccgc cacctttgcg acatgatcga tgtgccggag caactgcttc acttccaccg
     4321 gcggcatcag tttgtaatag cgctgaagaa ccgcccgcgc caactcccaa cgcttcgggt
     4381 tggacgagtg caatgccagc gcttccttga cttcgcgcac ccgctccggg cggttgccga
     4441 tgtaaacatc gtaggcctgc ttatccaggt accccgcatg gaaattgatg cacacgacgc
     4501 ctccccgccg ggcaatggcg cgaatgatat cgtcgttcat atttcgtgaa atatcgcaca
     4561 gcgctttcac ggaggaatgg gacgcgataa caggtgagcg gctgacttcg accgcgtcca
     4621 gcacggcctt gtccgagatg tgcgaaacat ccaccatcat tccaagacgg ttcatttccc
     4681 gcacgacatc gcggccaaac gccgacagcc cgttatgaac gggaacgtcc gtggcggcat
     4741 cggcccagtt gttcgttctg aagtgggcga gcgtcatgta acgaacgccg agccggtgat
     4801 agaggcgcag caaaggcaag ctgtccagga tgaggtggcc gccctccaga ctcaccatca
     4861 ccgcaatctt gccctctttc cgaatgcgga caatatcgcc ggcggtgtaa gcgacctcga
     4921 catcggcggc attgcggcgc gcctcttcgt gaaatgcgtc gatgcattgg agcgcgcgcg
     4981 gcagccaaag ctgctgagga tattcctgcg gctccacgta aaccccgaac aggatcgcac
     5041 ccacgcgccc gcgcttcatc cgcgggatat cggtttcgta ataggagtgt tcttcggccc
     5101 agcgataacc ttcatcgacg gtgtatctcg gcgtgtcaat gtgggcgtca aagatcagga
     5161 tgtcgcgcat gagctgctca aaccgctcct gcagattacc ggcttgcagc aatagcagcc
     5221 ccagaatgaa agacaacggc atgcagggga ttatacatcg gcggacaaga ggtgcttcgc
     5281 ctgttaacat ccgggccctg ccggcagtaa tagaacggga gcttgtatgg gtggcatacg
     5341 tcgagcagat gagtcaccat gaaatttgtc cgtgctggcg gcgctgctca ctccgggagt
     5401 tcccgttggc ttcgacccca ttttgaatct gccgccggta atcgccatgg cggtgatggg
     5461 ctttgtgcta ctattgctgc ccataggtac gtctgggcac gagaataact atgcgaatcc
     5521 tggtcccact ttttctggtg agtatctctt ttagcgcgtt tgcgcagcaa ccccgcacac
     5581 ccgctcccaa caaagaattc gaagccttac aggctcattt cgaacgggtg atcggccggc
     5641 gattcgatca gatcttccgc ggcattacta cagtcgaaca atgggagcag cgcaagccga
     5701 agcttcgggc cacgctcgaa aagatgctgt ggcatgattt ccgctggccc gatacgccgc
     5761 cgccggccac catcacttac aaagaagacc ggccgggcta cacgcttgag aatgtcgtta
     5821 ttgagaccgc gcccaagttg tttctcaccg ccaacctgta cctgccgcgc aacggacgca
     5881 agccctttcc cgttgtgctc tatcagtgcg gccatgccaa caagagctac tacgcccggc
     5941 acggcgcgtg gttcgcggca aacggaatcg ccacgctggt gatggacaac atcgagatgg
     6001 gcgaagtgga gttcacacac cacggcgttt attcaaacgc ctggttccac tggtacagcc
     6061 ggggcttttc cccgctggcg gtggagctgc tgaatgcccg ccgcgcggtg gactacctgg
     6121 cgtcccggcc cgatctcgac cgtaatcgta tcggcgcgac cggccgatcg ggcggcggaa
     6181 tgacaacgtt ttttctggcc gccctcgatg agcgaatcaa ggcttccgct ccggtgtcgg
     6241 gcacgctgtc caccaccggc tgggttcggc agcggctcac cgcggcgcac tgcgattgcc
     6301 agtatccggt gaacagccat ggcatgctct attcggaggt aggagcttta attgctccgc
     6361 gcgcgcaact gcaatgcaac gccgattcgg atcgcggatt tcccatggac gcgttcaacg
     6421 agatggtgga aaagaccaga gaggtctacc atctttacaa agcggacggc gcgctgcgca
     6481 ccgccatcgc gcccggaggt catgccgata cggaagcgat ccggttgccg gtctattcat
     6541 tttttctgaa agagtttctt ggcaaggaaa cgcctgtggc cgccgaaggc cccatcgaca
     6601 taccgcccgc cgaacagttg gtctgccacc gcaacgggtt gccgctggat gaaaagctga
     6661 cgcgcatcga cgaagaactc atccctccga gaaccgcttt ccagcagacg cttacgggca
     6721 aagccagaga acaacgaagg aaggagttga ccggcgcgtt gcgtgaagaa gtcttccgct
     6781 atttcccgga acagccggcg gcgctcgaac cgcagtggga agcggagacc accatccaag
     6841 gccgcacaat tcgaaaagtc agcttcactt cattcgaagg actgcgagtg aaaggtgttt
     6901 tctcggtccc cgcgggctcc acgccagccg gtaagctgcc gggcctcctg gttgtagacc
     6961 atcgcaaagg cattccggtg tggggcaacg agcagccgct cgagcgcaat cagtggggcc
     7021 gccgcgccgt gttgatcgtc gagactctag acacaggaag ccgcgtgctg gaacagaatc
     7081 ttcgcagctt cagcgacaac gatcttctcc accatatgaa gcggcaggcc atggtagcgg
     7141 gtacgacgct cgaatccatg caggtctacg aaatcctgcg atcgttggag ttgctccgca
     7201 cactgccaca ggtcgattcc gccaacgtaa cgattaccgg aaaagcggaa gcgggagtga
     7261 acggcatgta tgcggctctg ctggatggca aggtacagca ggtagtcctg cactccccca
     7321 cagcctcgca tatacagggt ccgcactatc tcggagttct ccgctatacc gatattccgg
     7381 aagtggcggc cctgctggcc ggcagtctcc gcgtctacgg agaagtcccg caggcgttgc
     7441 gcgcttccct ccgttgcgcg tctctggcgg actgcctgcg ataaatccgc tctgacagta
     7501 ctctggtatt gaaacaggtt cttcgagact caaatggaat tgcaccaggc tttgctcggc
     7561 gctggtgaag cgctgctgat cggcttcctg attggagcgc agcgggaagc gtcgcgcgga
     7621 gaatcggaaa aacagcccgg catacgggac ttcacgctca ttgctcttgt tggcgcactt
     7681 tccgctttac tgggaagcga tgcacttgcc gtctgcgcgc tggttgcact ggccgtactg
     7741 ttagcggtct atcactacca ggtgaaggag cgttccggcg tcactacgga agtggcggcg
     7801 gtaacgacgt tcttcctcgg ttacctgacc gccacgccgc ggttgcagca tggaacgctg
     7861 gtcgccgttt ccctgacgat cgccgtactc ttcctgttgg atgcgaaagt aactctgcac
     7921 aacttcgtcc ggagaacgat caccgagacg gagttccggg atacgatccg ctttctcgcg
     7981 ctggtgttca ttatctaccc ggtgcttccg gaagagccga tggggcctta cggctttttc
     8041 catgcacgga aggtctggct atttgtgatt ctggtctgtt ccatttcgta tttcggatac
     8101 ttcctccgga agttcgcggg tgagcgcgcc ggagccaacc tggccggagt gctgggggga
     8161 atcgcgtcct ccaccgccgc gacggtggct tttgcgcgaa tgagcggcac cgcgtccgat
     8221 gagtccggac acgaggaagg cgcggccatc atttcgaaca cgctgcaatt tccaagaatt
     8281 cttgtgctgc tctacatcat caatcaccag gtggcgcagg cctgcgtgtg gccgctgctc
     8341 gcgatgaccg cagccggcgg tatattttcg ctggtacggg cacatggacg gaacggccgc
     8401 gtcacgcctc ctactttcat caacaacccg ttcagcctgg ggccggccct gacttttggc
     8461 gctctcctga cagcggtact ctttctcagc agggcggcac tcgaagcgtt cggaaccgga
     8521 tcgatccagt gggtcggact gctgagcgga ctggcggatg cggacgccgt tttgttagcg
     8581 gttgcggaac tctacgaaca agccggcatt tcgatcggag ttgcttccgg taccgtgttg
     8641 ctcgcgctgg ctgccaatgc aatcaccaag agcgtcatcg cggtcatagc tggtacgcgg
     8701 cggtttgcac gcggcttgct ggtggcattc gccgggatct ttaccgcggg cggagtggcg
     8761 tggctcgttc aatccgccct gcaatagttc tatacgctca ctacggcgca cggagaatgc
     8821 tgaataatgg cgtaggcgtt ggtgcgcaag cggccgagaa aacggttagc ggccggacgg
     8881 ccgatcacta aaacatcggc cttgagttca ccggccgcgg cgctgacagc ctttcccggt
     8941 tctccgccta ccagtttcac ttccgcactc acaccggtat cggtattcag cttcccgatt
     9001 cgctcccgca ggacggcgcc cacgctggcg cgccattcgg gatcgtggac cacgccgaga
     9061 atcggctcta gctgcggact tgcgtggatg actgtcaggg ctgcttcaaa ctggccggcg
     9121 aaatcgctcg cccagcggag aattcgttcg ctctccggac cgagatcgat tgcgcaggct
     9181 acccggcgca cggctggcga ggttgcgtcg tcctcgattt tcatgtcatg aacaacggtc
     9241 cacaccgggc aatcgacgtc atgaagtact ttcgccgtca cgctgcccag cagaaaccgg
     9301 cgcacggtgc cgacgccgcg agtaggcatg atgatgacat ccactcccgc ctcgcgggca
     9361 tactcgacga tcttttcagc gggatcgccc tgcagcacgt gttgttgcac gcttagtccc
     9421 gtcagaatcg attcgaggcc ttgcacaaca cgtacggcgt cgccgccagt ggcgcctgcg
     9481 atccagccct ccttcgatgc tgggccgact gttacgccat taccctgccc tacatggagc
     9541 agcgtaattc gggtaccggg ctgactcgcg agtttccgtg catcggcggc tgccgcgagc
     9601 accgggagtg aaaagtcgac aggtataaga atatgtttca ggttcatcac gaatcatcct
     9661 ccctgctaca tcgctatcac gggacacggc gactcccgga cgatggagta ggcttgcgtc
     9721 cttaatcctc cgaaaggctt attcaaacgc ccgcgtgcaa tcgctgtggc acaggccttg
     9781 tggcgggctg cctccgcggc cactcgccgc gcaatgtcac cacctgcaag acacaggtct
     9841 acgtcgatgc ctgcctcttc gcgtacacga ttccacttct cgcgagcctt ctcaaacagg
     9901 tagtcgcgga ccgcaatggc gccggggttc ctggactcct cttccaccgc cggcacaaca
     9961 tgaataacct gcaacacggc ggagagtccc gccgccaact gtgaagccca tcgcagcaac
    10021 tcggcgcttg ccggctccag gttcgtggca cacaagagca ccggagatgc cggcgctttc
    10081 tgttcaccgt cttgttctgt atgagcgctc gtccagacag ggcaaggaca atcatacaag
    10141 accttggcgg taaccgagcc cagcatgtaa cggcggaacc gccctgttcc ccttgtgggc
    10201 aacatcacca gttccacctt ctcacgctcc acaaaagagg tgattaccga ggccggttct
    10261 ccatcctcca ccacagagct gaccgtttcg tagttgagat gctcccttac gaaatgattc
    10321 aagtgctcct gccccgatga cttcatctcc gcgatctctt ccggcagcca gaccagggaa
    10381 gcgtccagat cggtgtaccc gcttcggacg atgttcagaa catgcagtgc ggtcaggcgg
    10441 gagttaaaaa gccgcgccat tttgagtacg tgaggagccg cctcgataca ggcagcagag
    10501 aagtccactg gaaaaaggat gtgcttgaac tctgtcatat gtaatccgcc gtgttccagt
    10561 gtactcgcac tccggcccgg cggggagcgt ccgcgatccc gtcagaagtc cacaacgcag
    10621 gcagccggat tgcgcccggc cgcctgcaag ttgcaaacaa cgagaagcag tgctctactt
    10681 cctgccggca ttcagcttac tccatccaca tcatcgacac gtagagaacg gcgaagagga
    10741 tcgccgtgcc aatcagcgcc ccgccgattt gcagcatccg ttcgcgcctg ttttcggacc
    10801 ggtattcggc acgcaagtcg gccaggcccc gaaacagcgg gctgccaggt tcgtctgttc
    10861 cggccgtctt cacggccggt ggtggcatgg cttgctccac cgaggattct acttgccgtt
    10921 tatccagaag gttcgttgac atactattct cctctttgga tttgctgcgc ttttaacggt
    10981 gcaatttccg taccaacaat ttccgctagt cacccgcgcg cgagcgcact gtattgccca
    11041 cccgccgccg cggaaaacac tccggctggt tgcgcaacct cggccccaag cgagtggacg
    11101 aaatggccga attgccaggg atcgttcgat tccggggccg gacgccatgc aacagcgtcc
    11161 gaccggatgc tgccgatgta tggcgcggca gtacgaagga tctcctcatg cggtagatct
    11221 tcggagaaac ggagcccttt gcgaggattt tgcatcgccc acaagaccgc cggtacaatc
    11281 cccgccgcta cctgcaaagt agtggcattt ccgtcgggaa ccgattgcct ggctctttca
    11341 atcgaaaggc tgctccccgt ccaccaggat tgatagcggt gtcccataat caatgcgccc
    11401 atggtgtccg ttccttccat gatgtcttcg gtcagaatcc ttcggacagg gtgtagctcg
    11461 taattgcggc ttcgcagttc ttcaagggac tgaagcgaag cgggacacgg cacataagca
    11521 taggcaacgg tcgggcggta gaggcggccc gtagcgtccc tgacggtcag cgcatgcgaa
    11581 aggctgaaac attcgccatg agcgatcgcc atgccgagta tctcgcgctc cggaacccac
    11641 gatcgaatga acgtgtccat gccgaatcgt ggcagaatca cctggttggc gggtccgata
    11701 tcgggaacca gtgcatttgc cggcagcgat ttttcatgag ttccccagcc cagctcacat
    11761 ggtgctacac actcttccca catcccttca acgctccacg tactcacaaa ctcgccggcc
    11821 gatttcggat gagcggatct ttgggtgtcc cgctcactac agtggatcac tttcacgcca
    11881 agttctcttg cgagacgcgc aaaatccctt cgttccagca accattcgag ctcgtaccgc
    11941 tgagcgtcag acagggcgtt ctcacgaacc gcccggctgg cgatgtcgag caatcctttc
    12001 ttcacgaacg cggagatcag gcccggattc gccccctggt caattacggc ggttgtgcca
    12061 ttactccaac gcccggccat ttcaagaacc cgtacatagc gccggtacag cgacctgtcg
    12121 aatagagaag cgccgcgcaa atccggtgtg gcatcccagg attcgaggga agcattcaca
    12181 tacagtactt cgttgtcgtg cgcccattgg gcgatatcga aaaagtcgat gctccacgcg
    12241 aggtcgataa tcaggccgcc ccggcccaca ttcgacgaaa gaagacggtg gagattgtcc
    12301 ggtgtgacgc gctcctgaat gaatcgcact ccacgcgcct gccacggttc aaatgcctgg
    12361 ccgcggtagt tgaaatcgac aaccgtaatg tttcgcgggg agattcgaag gtgtttcagc
    12421 agaatcggca agacggattg ggcgacggcg ccaaaccccg ttatgacgat tctgttatcg
    12481 aaatcaacct tgtacactct tgtctcctcg tgatttgttt ggttaaccgc ggtttgcgcg
    12541 agcggcggag gttcacgaaa gaaacccgta aggtaggcag tcaggcgtgt tcagaagtgg
    12601 tttgacagag gtgtgcgtcc agtgggccat tgaggtgtca aactggtctc tacgcgcctc
    12661 cgctgcttgc tcaactgcag tagtgcaatt cccttgccac tgcatgacag ctcaaaacag
    12721 ggttttcgcg tatgtaagcg aacacgcgat gtccgttttc gggcatcgct gtccctttga
    12781 gggcaggagc ggacacgttc cgctcgatct tatttggaga ggcgctttac cgcttcggaa
    12841 acgtggcgca caatgcgtcc gtcatatccg cggtctttca agtcctgccg caacttctca
    12901 aacgctgcca gcgcatctgc acgctgaccg ccgatttcca ggatactggc gtggatgaat
    12961 tcacggcggg tctgccgctc attcccttga ttctgcccat catccagcca gaggtaggtc
    13021 tcccaggcgt ttttcggcgg aacagccgcg agagattgat tcagttcctc aaccgtgttt
    13081 acggtcgggc tgtaggctgt gaggatgcgg cgtctgtcgc gatcgctcag ggattcgccg
    13141 ttccttctca tttcatccgc cactcttatc agcgccgatc gaagatccgg gtgagaattg
    13201 tacgccatag cgccgagttg cagccggcgg accagcggcc tttctttgtt ggtctgaatg
    13261 gccagatcga aatgatggag cgcctcgctc acatcaccgt tcgtctgaat gatccagttg
    13321 ccaagcatcg catgcgcgaa aacgttcgat gggtcgatct tcagagcgtg acgcagatcc
    13381 cgctccgctg cggttccaaa ctctttgtaa gccaggtgct ggttcagcca atgtgcccac
    13441 ccgagatgag ccaggacgtc cgctgccctc tgtccttgca acttcgttcg cgccagtgcg
    13501 gcatcgagca ccagcaggat ctccgccaga cgcggcccgg caatgtcttc cgccttggag
    13561 ccttcattca ccaccacgct gaaattttgg agccatcgca tcgccatgtc agcctggaga
    13621 tcaagcgcgc ccggattcga cggatcgatt ttcaggagat tccggcaggt atggaaggca
    13681 gactcatatt ccgtccgatc ggcctgcacc cgggcggtgg ctaaaagcgc attgatttcc
    13741 gccctgcgag cccggccttc gcttcgcggg ccgatggcca tccagcccgc caacacgatc
    13801 agaacgaaga gcgcggccac gcctccaaaa acgagcgtct tcccgcgtct ggtttgatgt
    13861 ttgacgcgcg agttgtccgg accaggctgc acgccgagcg aattcgccag gcgcatgcaa
    13921 tcttcccgga acgtgctgtc ccgcagttcc gctgcctggt aaagcgcgag cggcgccaac
    13981 cggtccggaa ggccggcagc ttgcggcatt cccgcgccgc caaccaggac gggaacgatg
    14041 gctatacctc gggcgagcgc agcttcgatc tcgtagcgca cgtaatcctg ctgctgcacg
    14101 tccgagcgtt cccgaagcat ttcgagccat cccggaccaa tgacaaccag cacgacatcg
    14161 caggccgcga tggtattgcc gatcaccttg gcgaactcct gtccgggcgc gacgtcgtca
    14221 acatccatga aaacgcggtc agcgccgaag acagccgaaa ggtgatcgca gattcgtccg
    14281 gcatacgccg ccgagtcggc acgccggtag ctgatgaaga tgtttcccgt agcccgctct
    14341 cctgggaaca ttctagcgcc gggaacccgg acatcatccc gactcaggca gaaacgacgg
    14401 catactgata ccacggaacg gctgaaactc accgcgaacc tggcagagat gcagttcgca
    14461 attgcgcgcg ccccaatcgt aggcgagccg cacgagcgca gccttttcta ccggcagtag
    14521 aaacagtggt gtccggccct tgtaatggtc gagttcccgc agaatcaggg cagccgcttc
    14581 ctgctcgccg cgtgccacac aaggtccaag catgttcatg gcgggatgca cgcaggagat
    14641 catgaatccg ccgatcccgc catcgttgct ctcgtaaacc gaaacatgcc agataccctg
    14701 gtggttttcg atgcaatagc agtaatcccg ctcacgcatg ataccgctga cttccaattc
    14761 gagcgatatc atggcgggca catccgccag agtagcctct ctaacccgat cgagctccgc
    14821 tacgcggaac ttcattcctg ttttagggac ggtgaggaac atgtcctgaa acgtgcaccg
    14881 ggggacgaag cccgcggtgt tgtagagcga gaacgaatcg agattgagcg cgctctgggt
    14941 caggcgcagc gcgggaaagc cgtgctgttc cgtgtaatcg atgatgaatc gaagaagcgc
    15001 tttccctgcc ccctgcccga aataattcgg gtgaacgttc atgatgccca acgatacgtg
    15061 acgctcccgc ggatggaaga agcagcttgc catcagcctt cccgtacgcg tgttttcagc
    15121 caccacggcg caacccggat ccagcatcgc atacacatca tagaagacct ccgtcgcttg
    15181 cggatcgccg tgaaaaatgg gaggccgccc atgcatctgg taccagtggt tgatggagat
    15241 gtaaatcagc tccgacacct ccgagcggtc ccggtccgtc atctcccgta tttttaagct
    15301 gctctctata cccggctgca tcgcggttca cctttccgtg acggatcagc atattgactc
    15361 ccggcctgaa ctgtcaacag gaaactccct cgccgcacct ccggacgttg tatagtcgtt
    15421 aatcgtatgt ccacacaaat cagcgaacag gtttcatccg gtgtgagccg gcggcgcttt
    15481 ctcggctcgg ctgccgcgac cggcacgctg gcactcgcac gtattccggt tctgagcgga
    15541 gctccggaaa atccggccgg ctttcagatc ggctgttaca cccgcccatg ggaccagttc
    15601 gactataaag ttgcgctgga cggcatggcc gaagccggct acaagtacgc cggcctgatg
    15661 actcacaaag gtaaatcgtg ggtcatcgtg acgaccgaca ccacgcccga ggaggccgcc
    15721 tccatggggg aggaggtgcg ccgccgcggc atggaactgt cttccattta tgtgggcaac
    15781 ttcgcggttg aaaaatcgat tgaggccggc gtcgcaggat tgaaacggct gatcgacaat
    15841 tgcgccgccg cgcgaagccc gcgcatgctg gtcagcggca cgggcaagga cgcgctggtc
    15901 gaggcttact acaaggttgt ggccgaatgc tgcgactacg ccgcgtccaa caaaatcagt
    15961 attagcgtca agccgcacgg aggcagcaac gctaccgccg cgcagtgccg caagatcgtc
    16021 gagatggtgg gcaagaaaaa tttcggcatg tggtacgatc cgggcaatat cttcttctac
    16081 tccgagggca aaatcgatcc ggtggaggat gtgcccgacg cgcacgggct gattatgggc
    16141 atgagcatca aggattacaa ggcccccaaa gaagtcatgg tcacgcccgg caccggcctg
    16201 gttaacttcc gcgaggtgct caagcgcgcc aggcagggag gcttcacgcg tggtccgctg
    16261 atcgtcgagt gcctcgaccg tggcgattca gccgccaaga tcaccgccga agcgcgcaaa
    16321 gcccgccagc ttctggaaga gttgaccagc gcctgagccg gccggccgat ggcttaccgg
    16381 tccggaaaac ccgggtgcgt tttgatctcc cggatttgca gagtatgccg ctgcgcgtgc
    16441 gtggaaatca aaaggaacca ctgatatgcg tcgcaccttt ctctttgcac aacgtgactc
    16501 cgcagatcgt cctgagtagt cctcgcgtat tccagcatct gcacacgcag ttgatggaaa
    16561 gctttccatc cttcccgcac atcgctcaat tgccgttttg gttgttccgc aggtatagcc
    16621 ttctggcgct gcgtccggtc gatgccatac caaagaacac tatcgtccga accggatcgc
    16681 cgggagcggt ccggcggcgc ggccatgg
//
LOCUS       DASTMK010000083        24368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_324105, whole genome shotgun sequence.
ACCESSION   DASTMK010000083 DASTMK010000000
VERSION     DASTMK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24368
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324105"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFQ79_03380"
     CDS             <1..880
                     /locus_tag="VFQ79_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0138720.1"
                     /translation="RFHEEIQTALNISEARLKENPEDAHAHYTAGVAYGLRANYNFLV
                     RKAWLDSLKDATEARKAHSRAASIDPDFVDAKLVQGVYDYVVGSLPWYYKMLGFIAGY
                     RGDRERGIQVLREVAEHGRQNSMDAKVLLAAIYRRERHPEAAIPLLRDLIRRFPHNYL
                     FRLELVQMYSDAGDKESALEVLSRIEKLHQSGAPGYARLDPGKIHYARGNLLFWYNDL
                     EAALDDLRRAVEHADEVDLNTGVMAWMRLGQTHDLLEQREEAMKAYRQAVDLAPDSEV
                     GKESRRYLSSPYRRRK"
     gene            complement(903..1247)
                     /locus_tag="VFQ79_03385"
     CDS             complement(903..1247)
                     /locus_tag="VFQ79_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0138721.1"
                     /translation="MSQELRLFKAEIFQALAHPTRIALVESLRQGETSAGQLIEQLGL
                     EQANASQHLAVLRSKQIVTRRKEGNQVFYSLRDPALIEVLDILKQYFYSHLSQTMSML
                     KEIRKEQTSSKR"
     gene            complement(1244..2104)
                     /gene="nuoB"
                     /locus_tag="VFQ79_03390"
     CDS             complement(1244..2104)
                     /gene="nuoB"
                     /locus_tag="VFQ79_03390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEU0138722.1"
                     /translation="MFKILQKTFRTGLVTTGYPDAPAVISEHFRGAPRIGFADWRDAR
                     PAAWACPTGALSLIDSGGVRQVTMDYGLCIFCGECAEADATGAIRMTREFELACRHRR
                     DLVITAEYDLDSDGIQCAPARIHQERREEPDETELERRAQDTIHTLLGRSLAIREVDA
                     GSCNGCEVEITALNNPVYDLERFGIHFVASPRHADMLLVTGPVTRNMELALLKTYRAA
                     PEPKVVVAVGACGISGGIFGHNYATAGAVDEVVPVDVYIPGCPPRPQALLYGILLAVG
                     RIAQKERTRE"
     gene            complement(2272..3858)
                     /locus_tag="VFQ79_03395"
     CDS             complement(2272..3858)
                     /locus_tag="VFQ79_03395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU0138723.1"
                     /translation="MTDGRTKKIGEVLASGGELFGLPWQDGEGEIYVDLRGTDPRPAA
                     EYLRSAGCRHVAVFAEDRTTEEFCAYLYYVFEHLQDTRYLALRARIDPDRPEFPSLAA
                     EVPALNWQEREIQDWFGIEALGHPNPRRVALHDNWPDVHPLRKDFPIDTVLPPFQGEP
                     HVYRPTLGEGVFRIPVGPVHAGIIEPGHFNFAVAGEPILYLQLRMFYVHKGIEKRFEQ
                     LPVHHAVFLAESVSGDSSFSHGTAFSQAVERIAGVQIPEAAGLMRVVLLELERIYNHV
                     ADIGAIATDVGFVIANAHASRLRESILSINEELTGSRLLRGVVCPGGVRRPWDRARVS
                     RTLGLLAHFERDFNDLAGILEASDSTKDRLERTGILHPEKARDLGIVGVGGRASGQDL
                     DVRRDHPYAAYDSLPVDVPVYQEGDVLRRMQVRIDELRHSLRLIREAAARLGFGPHRM
                     PVPPLPAGRHAFGAVEGWRGEILYWLRTGPGNQIARCKVKDPSLNNWPALVEAVQGNI
                     VPDFPVINKSFNLSYSGTDR"
     gene            complement(3855..5351)
                     /locus_tag="VFQ79_03400"
     CDS             complement(3855..5351)
                     /locus_tag="VFQ79_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0138724.1"
                     /translation="MTIPILLLIPLVASGLTALAKSRRQMEILHLLSAAGGFAAAVFL
                     AAEVLARGEVHAWNGFVFADHLGALVVLLTAFVYLACAPYAVGYFRTDERNEVFGSQD
                     GEGSHLAKLRKYYSLTPLFAFSMHLVAVASNLGVMWVALEGTTLASIFLVTFYGRRTS
                     LEAAWKYAMIGGVGLSMALFGTILTYYAGRDVLGSDGLGGLNWTVLTKSAAGFDHTVM
                     RLAFILVLLGYGTKAGLAPMHTWKPDAYSEAPAPVAALMAAAVLNCALYALARFYILT
                     VHCLGPAFPSQLLMLFGFLSIGIAVPFILVQRSYRRLLAYSSIDHGGIMVLALGFGGL
                     LGTLGMVLHMTFHSVAKPLLFFCAGNAQQETGNDSLKKTASGLVHALPVSGPMFLLGA
                     LAVTGTPPFSLFQSEFMILQAGFAAQHVGLSIVFVSLLVAIFAGFFYHIAQLVLGPPD
                     ESRRGDTCRWKTFPVMGLSALTLLLGFWLPAPLYSLIEGAARILAVQP"
     gene            complement(5360..6046)
                     /locus_tag="VFQ79_03405"
     CDS             complement(5360..6046)
                     /locus_tag="VFQ79_03405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEU0138725.1"
                     /translation="MDFWQDTLFGDRMITLMAALVLVLQITMVAQRWLITNIRAFAIQ
                     SLLLAGIANTIAYFNSAPHIYIAAALTLLFKAILLPVILQRLVTRMDIRREIEPFVNV
                     PVSVIVCGLLTLLAYMVAEPLQEQPGIRTAASLGRNTLPVAIALFLIGFFMMVSRRKA
                     LTQVLALLSLENGLFLAGISLTYGMPLIVELGIFFDVLVAMLILAILVYRIRETFDSM
                     DVSRLSRLRG"
     gene            complement(6049..7005)
                     /locus_tag="VFQ79_03410"
     CDS             complement(6049..7005)
                     /locus_tag="VFQ79_03410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HEU0138726.1"
                     /translation="MIGQFLFVIAQAALVLLFAPLITGFSKTLKARLQTRRGPGILQP
                     YRDLHKLLRKGMVLPETASWIFSATPFVLFAATLLAGLLIPTVAAHAPLSLFGGILAV
                     VYLLALGRFFLALGGLDAGTPFGGLGSSREMTISAIAEPALMLAVFTVALGAGSTNLS
                     TMAQAAVGQSWRFLAPSQMLAFAALFIVLIAETGRIPVDNPATHLELTMIHEAMILEY
                     SGPYLALIEWSASIKQMILMTLLVNVFFPYGLSADGSVMGVAASVVLFVFKLAVLAIA
                     VVLVETGNAKLRLFRVPELLALAFVLSALALISTFLFGGVTG"
     gene            complement(7002..9044)
                     /gene="hyfB"
                     /locus_tag="VFQ79_03415"
     CDS             complement(7002..9044)
                     /gene="hyfB"
                     /locus_tag="VFQ79_03415"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEU0138727.1"
                     /translation="MGPISQFYTASLFVFAFGVAGGLAAHWWARSSRWTPLLCYAALA
                     GALLEACAAVGGLLNGSELAWPYPSGVPYLHYTVRLDPLASFFLLILSLLAASVSAYS
                     AGYWKHGSTGKNPALSCALLNLLLLSLTLVFTASDVVFFLIAWEVMAAAAYFLVVTDH
                     QKEEARKGGLLYILMSRAGTGMLFIGFLLFASAVGTTDFRAMHAAGEKLPGAAGGVAF
                     LLLFLGFGVKAGIIPLHIWLPAAHPVAPSNISALMSGIVIKTGIYGMARVFFDFYGSV
                     PAWAGMLVLLVGVVSALLGVLYALMEHDLKRLLAYHSIENIGIILMGFGAALLFRSYG
                     HPALAAVALIAGLFHTLNHGVFKCLLFLGAGSVLHATRTRNMEKMGGLIRRMPWTALY
                     FLVGAIAISGLPPLNGFVSEWLTYQSLLAGFGATKEITRVAFPIAGALLALTAALAAA
                     CFVKAFGIAFLALPRSREAAEAQESAPSMRAGMAVLAVACVLLGVGATWFLPVFYPIT
                     RQAFGVGMPGLVVGNGFVLTAGAPASGAVSTFVLALMLVVLGGIPFFLWLRRGREGKC
                     STGPTWDCGLPGLTAENEFTAMAFSKPLRMIFAALFRPRRAIQAEFDVSPYYPKAIHF
                     ESEIQPAFEKHFYDPLREGILRMARRMRPIQAGSLHAYLAYIFITLILLLLFGVRG"
     gene            9406..9711
                     /locus_tag="VFQ79_03420"
     CDS             9406..9711
                     /locus_tag="VFQ79_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138728.1"
                     /translation="MPDCAQRSTGRGCDFTVVIREAQWPCSRYDGGMNELVFEVVQEA
                     DGGYCAECLTESIFTQADSWDELRRNVLEAVNAFYFDTSAPASVRLRLVRDEILSVA"
     gene            9708..9947
                     /locus_tag="VFQ79_03425"
     CDS             9708..9947
                     /locus_tag="VFQ79_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU0138729.1"
                     /translation="MKLPRDISGQQLADVLCRRWQYSRVHQVGSHIILETSDPAHQRV
                     VIPAHASLRIGTLNSILRAVSQHKGVSRDVLIESL"
     gene            10722..11561
                     /locus_tag="VFQ79_03430"
     CDS             10722..11561
                     /locus_tag="VFQ79_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138730.1"
                     /translation="MAEARVYPERPLIQMFLAGYEQGAWKDATLNWVEEAQENAVEVI
                     ATRSDGKTLALEHTLIELFVGEKYDSTIFTEAFEPRIEKNPDFIIPGRALNVKIPVGA
                     LVGIKDRDAAVEALLAWMRASHASIPPGDSEQTIMVGGSVPLKVKFNSRDCSGDVGYC
                     WLARCDKPGNIDKIVEKAIKRKVPKLVRTQANKGILLLQREHITMGDTEILDLIEKFA
                     PEYPDIAKVDEIWFANTSIMESEGWVYLSRLHRGPTQIMSFHSGVLKHRRDDALGLNE
                     SVN"
     gene            12302..13339
                     /locus_tag="VFQ79_03435"
     CDS             12302..13339
                     /locus_tag="VFQ79_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEU0138731.1"
                     /translation="MQLRQTSSLISVLATLLVGTMAGLPAPAKAEKSPFAHVSMKVFA
                     DLLKIPLEPPEVAPTVHSAREEDGLVIEDVSWPSIGGDRVPAYVVRPARVSGRMPVIV
                     CLHGSSTNRDVDVAPKYEYGEWTRYGTNRKGKTLYGWARELARRGYITLAMTQRGLDN
                     RIPDTESRNKELLVRGRNVMGTIVYEIRQAVSYLQQRPDVDPSRIGMTGISFGGITTF
                     YTWLVDDRITAAAPICGGIGSVEKFIAHGARTYHGIYWWVPDLLTKGDHGEFAAAMAP
                     RPLMLWAPTDDIGMPNEGIDRFLEVAMPAYKQAGASDRLVVHRPPGQHALTMESVDAA
                     FHFFEKFLKGI"
     gene            complement(13412..13861)
                     /locus_tag="VFQ79_03440"
     CDS             complement(13412..13861)
                     /locus_tag="VFQ79_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0138732.1"
                     /translation="MKILIAIDGSECSSDTVAGVLQIPWPPDTKLRVLTVAGQKGVPS
                     MLEVMSSGGSLSAVREDTKSIAEAISNSAAAELRSAGLAAETVVLEGDPKKLIPEEAA
                     EWGADLVVAGCYGHSGVKRLLVGSVAQAIAATAPCSVLIVRRRQDSE"
     gene            complement(14009..15028)
                     /locus_tag="VFQ79_03445"
     CDS             complement(14009..15028)
                     /locus_tag="VFQ79_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138733.1"
                     /translation="MGFTRVFGSCLAVVFLASTGSAADTTPQREIYIPVEFTSLNSPD
                     YWSNGFWVGYKRSWTPGHVPEIRTFDRNGKEVIARKPLSLPDAYHTAVHAIAADRDGE
                     VVVSAEFWRSQGDMAPVLCRMNRAGEVTLMARTESFLGRALAVSPDGGIWSLGSRIAQ
                     PFEPKKDYEVLERYDASGNLKSRALPRSVFAVKYDPAVIHDLGEPQVVASSTRAGVMS
                     PGTKVWAELNASGEVIDRFTIEPPVSNDGALAELGRLVMSQDNEVYAFFAVVRRAGST
                     QPHFYGLYRLNRSAHRWDRVNTTPGPNFGGLYGIDRDSLIRRMGCCTYGWFPLSPVES
                     ASLGR"
     gene            complement(15089..16126)
                     /locus_tag="VFQ79_03450"
     CDS             complement(15089..16126)
                     /locus_tag="VFQ79_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138734.1"
                     /translation="MRSLVVFLAGVLPACAGNAAQERLPQGPGLAAQYRGDAGIRTHP
                     AVVFAEDFEVEEIEGFRARWSHINNKAGGALARTGDVPEASRGKRSLKMTATRGENEG
                     GHLYRRLPPGHMRLFLRFYVKFAADNGYNHHFVSLGGEIDPAPYPVGRAGLRSVNRWN
                     SGIEPAGIWSQTSPRTHFAPPGIWQFYTYWPDMRSFQHEDGSGTVFYGNEFTPAKPVP
                     AERGRWIAVEVMVKMNSSPEVSDGEQAFWIDGKLAGHFGPGTMKGRWSRALFRRDENG
                     ESFEGFRWRDDERVSINKLWLSHYVSEAAFKRTDAWLAGNPEAKVNTRSATVWFDDLV
                     VATEYIGPLTK"
     gene            16269..17105
                     /locus_tag="VFQ79_03455"
     CDS             16269..17105
                     /locus_tag="VFQ79_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0138735.1"
                     /translation="MSIAEFTALVFAVSSFAGFLGALTGLGGGVVITPMLVLALGVDM
                     RYAIGATLVSVIATSSGAAAAFVKEGFSNIRVGMFLEIATTLGAIAGAQLAAKAPVEV
                     LSVVFGLVLLYSALISAREQPKSGCAEEPDPLATRLRMNGSYPTSAGPRHYVVQRVKV
                     GFGMMFGAGILSGLLGIGSGALKVVAMDRTMRLPFKVSTTTSNFMIGVTAAASAGVYL
                     NRGYIDPGLTMPVMLGVLLGSVAGARILSRTRARALRVVFGVVVAVLAFQMIYNGLKG
                     GL"
     gene            17106..17492
                     /locus_tag="VFQ79_03460"
     CDS             17106..17492
                     /locus_tag="VFQ79_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEU0138736.1"
                     /translation="MFREWTDEDVDGLLGGLLRVGVVLAAIVVLAGGVVYLLRHGTEP
                     AGYGVFYGEPDDFRSPSGILRGALSLRGRGLIQLGLLLLIATPVARVAFSVAAFAIQR
                     DRMYVAITVMVLLLLLFSLFGARLPV"
     gene            complement(17500..18159)
                     /locus_tag="VFQ79_03465"
     CDS             complement(17500..18159)
                     /locus_tag="VFQ79_03465"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0138737.1"
                     /translation="MMIELEPESIGTLTKEGELERVYRLLKSWLVEGELAPGQVLSEV
                     DLARRCESSRTPVREACSRLSQDGWLSRVRHKGYIVTPVSIADLLQLYEYRKILECFS
                     ARKAAELALPEQLDALEEVIAPEVTPGADITQVVRASDTFHLGIAQIAGNQRVLHQLR
                     LTLEYQHRLDCLSTRRDPYWIPHGEIVAALRSRRGAEASEAMAAHIEHARDRMLKLFG
                     R"
     gene            complement(18165..20348)
                     /locus_tag="VFQ79_03470"
     CDS             complement(18165..20348)
                     /locus_tag="VFQ79_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEU0138738.1"
                     /translation="MNRRSFLQAGAAAAAARSLSAQSKGSVRKLAVEARPGGLTAVHT
                     MVDMPEWASDLGKYFDPKKFVQLCREAHVDVIELKTKNAVGDAMFPFKGRPCHRDWTT
                     ETAAFAKEAGIQFIAYYNVGLDNWYAVRKPEWRCLDPKGQPKIGFRAYNWMCIRSPWR
                     DVVLSEVRQVEEAVKPPGMWFDILGMPNAYGLGSYDPGAACFCEYCRAAYKSQFGEEM
                     PAASDDWEIRRRVNRFGHEGRIRMFRDLTDMLLRIDPGMEMGYNGAGNYDELNFTPVD
                     LRNRVTYNSSEAKQHRLISFTAKILQSLGKRYQIHTYGGFIRMQPSAVTGTWAAWNLI
                     PPPYLDVTAAVATAHAGRICIGVNPLPDGKVYEDEFRNIGQTLEKVKEREPWLRGLRS
                     VPGIAVLYDAQSDLAVRPNPGTPATPSRTEASGLHNALTEGGLHFDVIPAHLLNPVEQ
                     RVLLLGNAFAPPPELRTAIERFLENGGLLIATHETSLRDGRGKRLPNFAWSDLLGVRF
                     KGVSPFSEANYGWLGDELRDSAPAYPVLFTTPVLEVECTTAKPLADLLYPEAHRTPEV
                     FTDGETSHTHWGKPTGMPLVTLNRVGKGQVLYIAGPIGSEIASRDDTWLKRIIVYAVK
                     KYGTNLPVTAQVPAGVQIVLGRRDAMHVVSLVNHYAGMVISGEAVPHPQVGPVAVEVP
                     VRVLGGAPKSVKAVDASEFRWEVVRGTVRMRANTIGHHALFILES"
     gene            complement(20352..23864)
                     /locus_tag="VFQ79_03475"
     CDS             complement(20352..23864)
                     /locus_tag="VFQ79_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0138739.1"
                     /translation="MPFPKLLSAIFVAALLSALSASAQESRGSITGRVTDSSGSAVPN
                     ASVAATEESTNVAVTGVSNEQGNYQILFLRPGNYSVSTTVPGFKTFQRGGVQVRIGER
                     IGLDIRLDVGDVTEKLVVTAETPLLETATANVGQVVDSRRVQELPTMHGSVRSLFYQF
                     AGAALAGGAYASAPKFQDPSRPASSSWLSFNGAPAGTTEFALDGVPNTQTANADLGSG
                     MSNQPPVDSIQELKLETSYDASVGHTSGTHISIVTKSGTNALHGTAYFFYRDPALNAN
                     TFFGNAANQPRAEFNYKRGGASLNGPVYIPKVYDGRNRTFFAYTYEIMDEASQGYPFI
                     GSVPPVDHRKGDFSALLGLGPQYQIYDPMTTAPAANGRFTRQPFPNNVIPADRINPIA
                     LKMAEYWPSPNVPGLADGANNYALQNLPAPNFYQDHIARIDHVLNEKHRLYARYAQYF
                     KTEGPYRDYFRNITTGKYFRARPFNIVVDDTYTPSPTLVIDMRYGYQRFGARNWSPSQ
                     GFDLSALGFSQSVIDQLSYRHPVATMFPGVNVSGLQGLQPEGTSGQVGDDIHSWMVDL
                     NRPTGNHFLKFGGEFRTYRKNNYDFGNGTPRFVFGSSFTNGPLDNSPTSPAGIGQGFT
                     AFLLGIPNSGMVDYNDSYAVQSTYGGAYLQDNWRVTPRLTLTMGLRWEFLGAMTERFN
                     RSVRGFDPIAQLPIAEAVRANYAASPIPEIPASQFDVSGGLLFAGAGGQPRGLYPAQK
                     RNFMPRVGFSYNPFQKTVLRGGYGFYYLDTGITSRVGPFQPGYSQQTLIVPTLDNGLT
                     YQATLSNPFPDGIQAPAGSNQGAMTFLGRPITFYDSDLNSPYLQRWNLNIQQLLPGSF
                     LVEAGYAGSRATAMRISKNYNGLPNSYLSTLPYRDQAVIDRLSAQVANPFYPLLPGTS
                     LAGRNVSVSQLLLPFPQFTSVATTTNQGYSWYHSMQVRAERRFSNGFSAQLTYTFSKQ
                     MEALTYLNADDPMPYRTISPNDRPHHTGLTLLYELPFGRRKPLLANGPRPLLAVISGW
                     QISSIMNQWSGTPLSFGDVILKGGIKDIPLSGSERSLNRWFNTSVFETAPARQLGSHL
                     FQGPLYYSGVRSDGVHQWDFSLMKYTNLSEGVRLQIRADAFNAFNHPEFTPPNTSVTS
                     SAFGTVTGETVFTRQLQFAVKLIF"
     gene            24031..>24368
                     /locus_tag="VFQ79_03480"
     CDS             24031..>24368
                     /locus_tag="VFQ79_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138740.1"
                     /translation="MTKAIDAHVHVWTDDRKRYPRATGEAQYQPDRFTPQDYFAYSKP
                     SGIARTVLIQMSFYRFDNSYMLDTMRAYPGVFSGVGIVDSSSPHPEKTMRDLAKHGVR
                     GFRITPGASP"
BASE COUNT         5078 a         7270 c         7392 g         4628 t
ORIGIN      
        1 ccggttccac gaagaaattc aaaccgctct gaatatttcc gaagcacgtc tcaaagagaa
       61 tccggaagac gcccacgcgc actacacggc cggcgtcgcc tacggactgc gggcgaacta
      121 caacttcctg gtccggaagg cgtggctcga ttccctcaaa gatgccaccg aggcacgcaa
      181 agcccattcg cgggcggcat ccatagatcc ggactttgtg gatgcgaagc tggttcaagg
      241 cgtttatgac tacgtcgtgg gcagcctccc gtggtattac aagatgctcg gttttatcgc
      301 cggatatcgt ggcgaccggg aacggggtat tcaggttctc cgggaagtgg ccgaacatgg
      361 caggcaaaac agcatggacg cgaaagtcct gctcgccgcc atttaccggc gcgagcggca
      421 tcccgaggcc gccatcccgc tattgcgcga cctgatccgg cgatttccgc acaattacct
      481 gtttcgcctg gaactggtgc aaatgtacag cgacgccggg gacaaagaga gcgcgctcga
      541 ggtcctttcc cgcatcgaga agcttcacca aagcggcgcg cctggatatg ccaggctgga
      601 cccgggcaaa atccattacg cgcgcggcaa cctgctgttc tggtacaacg acctggaggc
      661 cgcgctggat gacttgcgcc gtgccgtgga gcacgcggac gaggtggatt tgaacaccgg
      721 cgtcatggcc tggatgcgcc tgggccagac gcacgacctg ctggaacagc gcgaggaggc
      781 catgaaggca taccggcagg cggtagacct ggcgccggat tcagaagtag ggaaggaatc
      841 gcgccgctat ctgtcttcgc cttaccggcg ccggaagtag acgggacgaa gggtgaggcg
      901 gctcaccttt tcgagctggt ttgctccttc cggatctctt tcaacatgga catggtctgc
      961 gacaggtgcg aatagaaata ttgcttcagg atgtcgagaa cctcgatcaa cgctggatcg
     1021 cggagagaat agaatacctg gttgccttct ttgcggcggg taacaatctg cttgctccgc
     1081 agcacagcta ggtgctgaga ggcattcgcc tgctctaacc cgagttgctc gatgagttgg
     1141 ccggcggaag tttccccctg gcgcagggac tccacgaggg cgatccgggt gggatgcgcc
     1201 agggcctgga atatctccgc tttgaacaga cgcagttcct ggctcattcg cgagttcgtt
     1261 ccttctgcgc aattctgccc accgcgagca ggattccata cagcaaagcc tgcggccgcg
     1321 ggggacagcc gggaatatag acatccaccg gaacaacctc gtcaaccgcg ccggccgtcg
     1381 catagttatg gccgaagatg ccgccgctga tgccgcaggc gcccaccgcg acgacgacct
     1441 tcggctccgg cgccgcccgg taagtcttca acagcgccag ttccatgttc cgggttaccg
     1501 gtccggttac gaggagcatg tccgcatggc ggggcgacgc cacgaaatgg ataccgaacc
     1561 gctccaggtc gtacacgggg ttgttcaggg ccgtgatctc cacctcgcag ccgttgcagg
     1621 agccggcatc cacttcccgg atggccagcg agcggcccag cagggtatga atcgtgtctt
     1681 gtgctctccg ctccagttcc gtttcgtccg gttcctctct gcgttcctgg tgaatccgcg
     1741 ccggggcgca ctggattcca tcggagtcga gatcgtactc ggcggtaatc accaggtccc
     1801 gtctgtgccg gcaggccaac tcgaattcgc gggtcatccg gatcgctccc gtcgcatcgg
     1861 cctcggcgca ttcgccgcag aaaatacaga gaccgtagtc catcgtcacc tggcgcacgc
     1921 cgccggaatc gatgagggac aacgcgccgg tgggacatgc ccaagcggca gggcgcgcat
     1981 cacgccagtc ggcaaagccg attcgcggcg cgccccggaa gtgttccgaa atcaccgccg
     2041 gcgcgtccgg atagccggtt gtaaccaacc cggtgcggaa ggttttctgg aggatcttaa
     2101 acatggcttt gcgctcctac cgcttgctaa gaaagtgtga agaaattgaa ttcagccacg
     2161 gatgaacacg gatgaacaca gataagtttc ctgttttcaa tctgtgttta tctgtgtcca
     2221 tctgtggctc cgaatctttc acaccttccg aggcccgcgg ctcggtttca cttaacggtc
     2281 cgtcccggaa tacgacaaat taaaactctt gttgataacc ggaaaatccg gcacgatatt
     2341 gccctggacg gcctctacca aggcgggcca gttgttcaac gagggatctt tgactttgca
     2401 tcgcgcgatc tggttgccgg ggcccgtccg gagccaatag aggatctctc cgcgccagcc
     2461 ttcgaccgcg ccgaaggcgt gccgaccggc cggcaacggc ggcacaggca tgcggtgcgg
     2521 accaaatccc agcctcgccg ccgcctcgcg gataagccgg agcgaatgcc gcaactcatc
     2581 gatacgaacc tgcatgcgcc gcaggacatc gccctcctga tagaccggca cgtcgaccgg
     2641 cagcgaatcg tatgcggcgt aagggtgatc ccgccgcaca tcgagatcct gtcccgaggc
     2701 cctgcctccg acccccacga tgccgagatc ccgcgccttc tccggatgca gaatcccagt
     2761 ccgttccaac cggtctttcg tggaatccga cgcctcgagg ataccggcaa gatcgttgaa
     2821 gtcccgctca aaatgcgcga gcaggccgag ggtgcgggag actcgcgcgc gatcccaggg
     2881 cctgcgaact ccgccgggac acactacgcc acgcagcagg cggcttccgg tgagttcctc
     2941 gttgatcgag agaatgcttt cgcgcaaacg ggaagcatgc gcgttggcga tcacaaaccc
     3001 gacgtccgtt gcgatggcgc cgatatcggc cacgtggttg taaatccgct ccagttcgag
     3061 aaggaccacg cgcatcaggc cggccgcttc gggaatttga accccggcga ttcgttccac
     3121 cgcctgcgaa aaagccgtgc cgtgtgaaaa actcgaatct cccgacacgc tctccgccag
     3181 gaagacggcg tggtgaaccg gaagctgctc gaacctcttc tctatgccct tgtgcacata
     3241 aaacatgcgt agctgtaagt agagaatggg ctcaccggca accgcgaaat tgaaatgccc
     3301 cggttcgata attcccgcat gcaccgggcc gaccggaatc cggaaaacgc cttcgccgag
     3361 ggtcggccgg tagacgtgcg gctctccctg gaagggaggc agaacggtat cgatgggaaa
     3421 gtccttgcgc aaggggtgca catcgggcca attatcgtgg agggccacgc ggcgcggatt
     3481 cggatgcccg agcgcctcga ttccaaacca gtcctgaatt tcgcgctcct gccagttcag
     3541 ggccgggact tccgcggcca gcgaaggaaa ctccggccgg tccggatcga tgcgggcgcg
     3601 aagcgccaga taccgggtgt cttgcaggtg ctcgaacaca tagtacagat aggcgcagaa
     3661 ctcttccgtc gtgcggtctt cagcaaacac ggcgacatgc cggcagccgg cggagcgaag
     3721 gtactcggcc gccggccgtg ggtccgttcc tcgcagatcc acgtagattt cgccttcacc
     3781 gtcctgccat ggcaggccga acagctcgcc gcccgacgcc aggacttcac cgattttttt
     3841 cgtccgcccg tccgtcatgg ctgcaccgcc agaatgcgtg ccgcgccttc gatgagagag
     3901 tagagcggcg caggcagcca aaatccaagc agcagcgtca gagcggaaag acccatcacg
     3961 gggaacgtct tccaccggca ggtatccccc cgccgcgatt cgtccggagg accgagcaca
     4021 agctgcgcga tgtgataaaa gaagccggca aaaatggcca ccagcaacga cacgaacacg
     4081 atggacaatc caacgtgctg ggcggcaaag cccgcttgca gaatcatgaa ctcgctctga
     4141 aaaaggctga agggaggtgt gccggttacc gccaacgccc ccagaaggaa catcgggccg
     4201 ctgaccggca gtgcatgaac caggccgctg gccgtcttct tcagcgaatc gttcccggtc
     4261 tcctgctgcg cgttgcccgc acagaaaaac aacagcggct tggccaccga gtggaatgtc
     4321 atgtggagca ccatcccgag cgtacccagc aggccgccga agccgagcgc gagaaccatg
     4381 atgccgccat gatcgatgct ggaatacgcc agcagcctgc ggtagctgcg ctgcaccaga
     4441 atgaagggca cggcgatacc gatagagaga aagccaaaca acatcaggag ttgggagggg
     4501 aaggcaggcc ccagacagtg cacggtgaga atatagaatc gcgccagtgc ataaagggcg
     4561 caattgagga cggccgcggc catcaatgcc gcaaccggcg cgggcgcttc gctgtaggca
     4621 tccggcttcc aggtgtgcat cggcgccagc ccggccttgg ttccgtagcc aagaagaacc
     4681 agaatgaagg ccagccgcat cacggtatgg tcaaacccgg cggcgctttt cgtcagcacg
     4741 gtccaattca gtccgcccag gccatctgag ccgagcacgt cccggccggc gtaataggtg
     4801 aggatcgtgc cgaagagcgc catcgacagg ccaacgccgc cgatcatggc gtacttccag
     4861 gcggcttcga gcgaggtgcg gcggccgtag aaggtcacca ggaagatcga cgccagcgtg
     4921 gttccttcga gcgctaccca catcacgccg agattactgg cgaccgccac caggtgcatc
     4981 gagaaagcaa acagcggagt caacgagtag tatttgcgca gcttcgccag atggctgcct
     5041 tcgccatcct gcgatccgaa cacctcattc cgctcgtctg ttcgaaaata gccgacggcg
     5101 tagggcgcgc aggcgagata gacgaaggcg gtgagcaaca ccaccagcgc gcccaggtgg
     5161 tcggcaaata cgaagccatt ccaggcgtgg acttcgccgc gggcgagaac ttcggcggca
     5221 agaaagacgg cggcggcaaa tcctccggca gccgacagca gatgaaggat ttccatctgg
     5281 cgccggcttt tcgcaagcgc tgtcaatccg ctcgccacca gtggaatcag caacaggatg
     5341 gggatcgtca tcgccgttgt caacctctca accggctcag ccggctgaca tccatggagt
     5401 cgaaggtctc gcgaattcga tagacgagaa tggccaggat cagcatcgct accagaacat
     5461 cgaagaagat tcccagttcg acgatcagcg gcattccata agtaagggaa atgcccgcga
     5521 gaaacagccc gttctcgagc gacagaagag caaggacctg cgtcagggct ttgcggcggc
     5581 tgaccatcat gaagaacccg atcagaaaga gcgcaatggc gaccggcaac gtgttccggc
     5641 ccaggctggc cgccgttctg ataccgggct gttcctgaag aggctccgcc accatatagg
     5701 caagcagcgt cagcagaccg cagacgatca ccgaaaccgg cacgttcaca aacggttcga
     5761 tttccctgcg tatatccatt cgggtcacca ggcgttgaag gataaccggc agcagaatcg
     5821 ccttaaacag caaggtgagc gcggcggcga tatagatgtg cggcgcgctg ttgaagtagg
     5881 cgatggtgtt ggcgatgccg gcgagcagca gcgactggat cgcaaaagcg cggatgttgg
     5941 tgatcagcca gcgctgcgcc accatcgtga tctgcaggac cagcaccagc gccgccatca
     6001 gagtaatcat tcgatcgcca aatagagtgt cctgccagaa gtccatggtt aacctgtgac
     6061 gcctccgaag aggaaggtcg agatcaatgc gagcgcgctc aggacgaacg cgagcgccaa
     6121 taactccgga acgcgaaaca atctcaactt cgcgttgccc gtttccacca gcacgaccgc
     6181 aatcgccagg acagccagct tgaagacaaa cagcacgaca ctggcggcga cgcccatgac
     6241 cgacccgtca gcgctcaagc cgtacgggaa gaagacgttc accagcaggg tcatcagaat
     6301 catctgcttg atcgacgcgc tccactcgat cagggccagg tacggccccg agtattcgag
     6361 aatcatcgct tcgtggatca tggtcagctc caggtgcgtc gccgggttgt cgaccggaat
     6421 gcggccggtc tccgcgatca gaacgatgaa gagcgccgcg aaggccagca tctgcgaggg
     6481 cgccagaaac cgccagcttt gtcccacagc cgcttgggcc atggtgctca gattggtcga
     6541 tccggctccg agcgccaccg tgaaaacggc cagcatcaac gcgggctcgg cgatggcgga
     6601 gatggtcatc tcgcgactgc tgcctaatcc accgaatgga gtgccggcgt ccagcccgcc
     6661 cagagccagg aaaaaacgcc cgagagcgag gaggtagacg accgcaagaa ttcctccgaa
     6721 caggctcagg ggcgcgtgtg cggcaacggt gggaatcaga agcccggcca gcagcgtggc
     6781 ggcaaagagc acgaaaggag tcgccgaaaa gatccaggaa gccgtttcgg gaagcaccat
     6841 gcctttgcgg agcagcttgt gcaggtcacg gtaaggttgc agaattcccg gtcccctacg
     6901 cgtctgaagc cgcgccttga gcgtcttgct gaagccggtg atcaggggcg cgaacagcag
     6961 taccagcgcc gcctgggcga ttacgaacag aaactgcccg atcatccacg caccccgaag
     7021 agcagcagca gaattaaggt gatgaagatg tatgcgagat aggcgtgcaa acttcccgcc
     7081 tggatcggcc gcatccgcct cgccatgcgg agaatgccct cccgcagcgg atcgtagaaa
     7141 tgtttctcaa atgcgggctg tatctccgat tcgaaatgga tcgccttcgg gtaataggga
     7201 gagacgtcga attcggcctg tatggcacgg cgaggccgga acagcgcggc aaagatcatt
     7261 cgcagcggct tggagaacgc catcgcggtg aattcgtttt ccgcggtcag accaggaagg
     7321 ccgcagtccc acgtggggcc ggtggagcac ttcccttcgc ggcctcgcct cagccagagg
     7381 aaaaacggga ttccgcccag cacgaccagc atcaacgcca atacgaaggt ggataccgct
     7441 ccactcgccg gtgcgcccgc ggtcagaaca aagccgtttc ccacgacaag tccgggcatg
     7501 ccaacgccga aggcctgccg ggtgatcgga taaaagaccg gcaaaaacca tgtggcgccc
     7561 acgccaagta gtacacaggc aacggcgagc accgccatcc cggcccgcat cgacggcgcg
     7621 gattcctgtg cttccgccgc ttccctgctt cgcgggagtg ccagaaacgc gattccgaaa
     7681 gctttcacga agcaggcggc ggcgagtgca gccgtcaggg caagcagcgc cccggcaatg
     7741 gggaacgcaa cgcgagtgat ttctttcgtc gcaccgaagc cggccagcag cgactggtaa
     7801 gtaagccatt cactgacgaa gccgttcagg ggcggcagac cggaaatagc gatggcgccg
     7861 accaggaaat acagcgccgt ccatggcatc cggcgtatca ggccgcccat cttttccatg
     7921 ttccgggtgc gtgtggcgtg cagcacgcta ccggcgccga ggaatagaag gcacttaaag
     7981 acgccgtggt tgagcgtgtg gaacagcccg gcgataagag cgacggccgc cagggcggga
     8041 tgtccgtagg aacgaaaaag cagggcggct ccgaagccca tcaggatgat gccgatgttt
     8101 tcgatgctgt gataggccag cagccgcttc aggtcgtgct ccatcagcgc gtacaggact
     8161 cccaatagcg ccgacactac ccccaccagc agcaccagca ttcccgccca cgccggcacc
     8221 gatccgtaga aatcgaagaa gacccgcgcc atgccataga ttccggtctt gatgacgatc
     8281 cccgacatca acgccgagat gttgctggga gcgaccgggt gcgcggcggg aagccagatg
     8341 tgcagcggaa tgattcccgc cttcacaccg aaaccgagaa acagcagcag aaaggcaacc
     8401 ccgccggctg cccctggcaa tttctcaccg gcggcgtgca tcgctcggaa gtccgtcgtt
     8461 cccacggccg atgcgaacaa cagaaatccg atgaaaagca ttccggttcc cgcgcgcgac
     8521 atgaggatgt aaagcagccc gcccttcctc gcctcttcct tctggtggtc cgtcaccacc
     8581 aggaagtacg cggcagcggc catgacttcc caggcaatca ggaagaagac gacgtcggac
     8641 gcggtgaaaa caagcgtcag gctgagcagg agcaggttca gcagcgcaca ggacaaggct
     8701 gggttttttc cggtggaacc atgcttccaa tagccggccg agtacgccga gacactcgcc
     8761 gccagaaggg aaaggatcaa caggaaaaac gatgcaaggg gatcgagacg caccgtgtaa
     8821 tgcagatacg gaacaccgga aggataaggc cacgcaagct cagagccatt gagcagccct
     8881 cccacggcgg cgcaggcttc cagcagggcg cccgccaacg cggcgtagca gagaagcggc
     8941 gtccatcgcg aactccgcgc ccaccagtga gcggcgagac cgcctgcaac cccaaaggca
     9001 aacacaaaca ggcttgccgt atagaactga ctgatgggcc ccatcaaatt gcgcgcggga
     9061 ggctaagaca aatcactctt tgcagtatat tcgatatttc gaatgttcgt caaaaccagg
     9121 gggtagctgg cggcaggctt cggctaagcc tggaagcgat gagcctggaa gactacaagg
     9181 tccgaaggcc tcccgacagt gaatacgaag cgctacgcga ttacttgtca cagctcaagg
     9241 ctcttgggca gcgccgtaac gacatcatgc atggtgtggt ctgttcggat catgtccgat
     9301 caacaaaggc gcgagcgaca tgccgctgca gcacatcccg gaaccatgat ccggagtagc
     9361 actgccttgt tccgtacgaa agcgggtggc aaggagggat gatgtgtgcc agactgcgcc
     9421 caaaggtcca cgggacgtgg ctgcgacttc acggtcgtaa tccgtgaggc gcagtggccg
     9481 tgttcccgtt atgatggagg gatgaacgag ttggtgtttg aagtcgtgca ggaggcggat
     9541 ggtggctact gcgctgagtg cttgaccgaa agcatcttca cgcaggctga ctcctgggat
     9601 gaactccgcc gaaatgttct tgaagcggtg aatgcgttct atttcgacac gagtgcgccc
     9661 gccagcgttc gccttcgtct tgtccgcgat gagattctat cggtcgcgtg aaactcccgc
     9721 gcgacatctc tggtcagcag ttggctgatg tcctttgcag gcgctggcag tactcccgcg
     9781 tgcaccaggt ggggagccac atcattctgg aaacctcgga tccggcacac caacgcgtag
     9841 tgattccagc acacgcctcg ctccgaatcg gaacattgaa ctcgatactg cgggctgtct
     9901 cgcagcacaa gggtgtgtct cgggatgtgc tgatcgagtc tctttgaagc ttctcggaag
     9961 ccgacttttc gagcctgatc tggctcaact accgagttgc cgaccatcca gacagaatgg
    10021 gacacttccc aaagcccgac ttctagatcg ccgtacgatg gccacgcatt gtacgagtgg
    10081 ccggtgattc ggacggagcc gcattgatgc cgtgtgctcc ggatctatga tcggcggcga
    10141 cgcaaggtaa acacgaactt tgtggagagg attacggcta caggctgacg ttcgcaaaat
    10201 cctgcttgcc ctgacaatgg gagatctggc agtgaactcg gttttgtgtg ctgggtcggt
    10261 gattgctgcg aggaacgcag accacgctgt tcgcatttcg gcttcaagct ttgggaccca
    10321 tttccagtgt tcccatttct tttgacgttg gaatgtgatg tgcgcgagtt gcttgtccat
    10381 ctcgtcttgc cactcggttt cccacgttgg aagcgtccat gtccgcgcga acccggctgg
    10441 caggtagtca ctggcgagaa tgtctggcaa ctcgacgccg ttgcgcgatg accgtttgtc
    10501 gctcatcaga aaatcccgca tcttccggta gccaagaagg aaggcgtcgt cgcagtgcgt
    10561 acgccatggt gcttgaccgt ggagatcgca atgagcggcg gaaatcaggt tggcgtattc
    10621 gtaacaaaca tgttcaatgc cgcgcttcaa aagctgcggc gtgatcgggt acggagtcgc
    10681 cacgacttaa ggtatcatcc atgtttctag gagaaaaatg gatggccgaa gcacgcgttt
    10741 atcccgaacg cccgctgatc cagatgttcc tcgcgggcta cgagcaaggc gcatggaagg
    10801 acgcgacgct gaactgggtt gaggaagcgc aagaaaacgc cgtggaggtg atcgcgacca
    10861 gatcggacgg caagacatta gccctagaac ataccctgat cgagctgttc gttggcgaga
    10921 agtacgactc gacgatcttt accgaggcgt tcgagccacg aattgagaag aacccggact
    10981 tcataatccc cggccgcgct ttgaacgtga agattcccgt tggcgcgctc gttggcatca
    11041 aagatcgcga tgcggcagtc gaggccctcc ttgcgtggat gagagcgagc catgcgagca
    11101 taccccctgg cgactctgag cagacgatta tggttggcgg ctcggtgcct ctgaaggtca
    11161 agttcaacag ccgagattgc agcggtgatg tgggttattg ctggttggcg cggtgtgata
    11221 agcccgggaa tatcgacaag atcgtggaga aggccatcaa gcggaaggtg ccgaagctgg
    11281 tcagaacaca agcgaacaag ggcatcctgc tcctccagcg cgagcacatc acgatgggcg
    11341 acacggagat tcttgatctg atcgaaaagt tcgcgcccga atatcccgat atcgccaagg
    11401 tcgacgagat ttggttcgcc aacacgtcga tcatggagtc cgagggctgg gtgtatctct
    11461 cgcgtctaca tcgcggccca acccagatca tgagcttcca tagtggcgtt cttaagcatc
    11521 ggcgcgatga cgctctcggc ctcaatgaga gcgtgaatta gaaagcggct agttcctcat
    11581 tggtgaacgg ccttttcttc gctccctgaa cggtgcgcgt gtccgttgtg ccccgtctat
    11641 cttgctgatt gaacagttaa atggttttgc gccaccattt ctcacgcgca tcgccatcag
    11701 gaccttcggg cagatggtgc tcggacactg gtgccgaaac ggcattgggg aagttgggaa
    11761 tgcttactgc acggacgacg ggcaaaccgg aactaatcca cccggacgta aaacggccaa
    11821 accgttgatc tgccgatcgt gcgggggcgt gacaacggat cagtaccgat aagcgcccaa
    11881 gcattctgca atccacaggg aggagttgtc gccatgttgt agtgcgccag ccttcccatg
    11941 caggatcaag agaatcgagt agtcgcgacg gccaaagttg ttttccggtc caccgaggtc
    12001 aacgcctgct ctacctgatg gggcgggagg gcactaggta aggtcgactg tttccagttg
    12061 accaccgccg gtacacatgc ggccccatct ttttcgatgt ctcccgatag cacgcgaatc
    12121 gattcgagcg cttctagacg cgagtttgca gcgagagcac ctagcatcgc atacgaatac
    12181 tgattgtgtc ttcgcccgtc ctatgaattc gatccagcgt ttacggcgtg ccgccggggt
    12241 cacgtgcgag cgagcattgt agccgggcgg ctggtataca atcacgttct cggatcaaga
    12301 aatgcaactc agacaaacca gcagcctcat atccgttctt gccactttgt tggtgggaac
    12361 catggccgga ttgcctgctc ccgccaaggc cgaaaagagc ccgtttgccc acgtctcgat
    12421 gaaggtgttc gcggacctgc taaaaatacc gcttgagccc cccgaggtcg ctcctaccgt
    12481 gcacagcgcg cgggaagagg acggtttggt gattgaggac gtctcgtggc catccatcgg
    12541 aggcgatcgg gttcctgcct atgtcgtccg gcccgcgaga gtctcggggc ggatgccggt
    12601 catcgtgtgc ctccacggga gctcaacgaa ccgggatgtg gatgtggcgc cgaagtacga
    12661 gtacggcgag tggacgcgct acggaacgaa ccgcaaggga aagacgctct acggctgggc
    12721 gagggaactc gctcgtcgag gttacatcac tctggcgatg acgcaacggg gcctggacaa
    12781 ccgaatcccg gacacagaat cacgtaacaa ggaactcctc gtgcgtggcc gcaatgtgat
    12841 gggaacgatc gtctacgaaa tccggcaagc ggtcagctac ttgcagcagc gcccagacgt
    12901 cgacccctcc cggatcggaa tgaccggtat ttcgttcggg ggtatcacca cgttttatac
    12961 gtggctcgtc gacgaccgta ttacggccgc ggcgccgatc tgcggcggca ttgggtcggt
    13021 cgaaaagttc atcgctcacg gagcgcgaac ctatcacgga atttattggt gggtcccgga
    13081 cctcttgacc aaaggcgatc atggggagtt cgccgcggcc atggctcctc gcccgctgat
    13141 gttatgggct cctacggacg acataggaat gccgaacgaa ggcatcgacc gattcttaga
    13201 ggttgccatg ccggcataca agcaggctgg agcatccgat cgcctggtcg ttcatcggcc
    13261 acccggacag catgcgttga ccatggaatc agtggacgca gctttccact tttttgagaa
    13321 gtttttgaaa ggaatttgag aacagccggc cgggggatcc ggggtgcgct cacttaacgc
    13381 tcacggctcc gctacatggc ttaggcagtc tctactccga gtcctgccgg cggcggacga
    13441 tcagcacgga acagggagcg gtggcggcga tggcctgcgc gacgctgcca accagcagcc
    13501 gtttgactcc cgaatgcccg tagcagccgg ctaccacgag atccgctccc cattcggcgg
    13561 cctcttccgg aatcagcttt ttcggatcgc cttcaagaac taccgtttcg gcagccagtc
    13621 cagccgagcg aagctccgcg gcggcgctat tcgaaatagc ctccgcgatg ctcttcgtat
    13681 cctctcgcac cgcagacagg ctgccgcccg acgacatgac ctccagcatg gaaggtacac
    13741 ctttttgtcc cgccacggtg agaacgcgca gcttcgtatc gggcggccac ggaatctgaa
    13801 gaacgccggc tacggtgtcg cttgaacact ccgaaccatc gattgcaata agtattttca
    13861 tttccacctc aactcaacag ccagcctggc gaatgcaaaa cctgccgcga aaccgcgagc
    13921 ccttccttcc cgtagtgcaa tccatgtcag attcgactgt gaaatacaga aaccggaaat
    13981 gggccatcca ccgcggcagt agccgcactc agcggccgag ggatgccgat tcgacagggg
    14041 atagcggaaa ccatccgtac gtgcagcaac ccatccggcg gatcagagaa tctctatcga
    14101 tgccgtaaag gccgccgaaa ttggggccgg gggtcgtgtt gacccgatcc catcggtgtg
    14161 cggaccggtt gagacggtaa agcccgtaga aatgaggctg tgtgctgccg gcacggcgga
    14221 caacggcgaa gaaggcatag acctcgttat cctgcgacat cacgagccgt ccaagttcgg
    14281 ccagagcacc gtcgttggat acgggaggct ctatggtgaa gcggtctatt acttctccgg
    14341 aagcattgag ctcagcccac acttttgtac ctggactcat cacaccggcc cttgtcgagg
    14401 aggcaaccac ctgcggttca ccaagatcgt ggataactgc cgggtcgtat ttcacagcaa
    14461 aaacggatcg aggaagagcc cgcgatttga gattgcccga ggcatcatag cgttccagaa
    14521 cctcgtagtc cttcttcggc tcgaatggct gcgcgatgcg ggaaccaagc gaccagatgc
    14581 cgccatcggg actcacagcc agcgcccttc caagaaacga ttccgttcgc gccatgagtg
    14641 tcacttcacc cgcacgattc atccggcaaa gaaccggggc catatcgccc tggctgcgcc
    14701 agaactccgc agacacgact acttcgccgt cacgatccgc cgcaatcgcg tgaacggccg
    14761 tatggtatgc atccggaagg gataacggct tccgggcgat cacctctttc ccgttccggt
    14821 caaaggtacg aatttcgggg acgtgtcctg gcgtccaact tcgcttgtac ccaacccaga
    14881 agccattgct ccagtaatca ggcgaattca ggctggtgaa ttcgaccggg atatagatct
    14941 ctctttgcgg agtggtgtcc gcagccgaac ctgtggacgc gagaaaaact accgcgaggc
    15001 atgaaccgaa cacgcgagta aatcccatga actgcctcca acctgacgtg tagtctattc
    15061 aatgggcatt gtcaagctat ctgttgaact acttcgtgag cggaccgata tactcggtgg
    15121 ccaccaccag atcgtcgaac cacaccgtgg cgctcctggt attaaccttc gcctccggat
    15181 ttccggccag ccacgcatcg gtgcgcttga aagcggcttc gctcacgtaa tgggagagcc
    15241 agagcttgtt gatgctcacg cgctcgtcgt cgcgccagcg gaaaccttcg aaggattcgc
    15301 cgttttcgtc cctccggaac agcgcccgcg accagcgccc tttcatcgtg ccagggccga
    15361 aatgccctgc cagtttgccg tcgatccaga aggcttgctc accatcggaa acctccgggg
    15421 aggaattcat tttgaccatt acctccaccg cgatccagcg gccgcgctcg gcgggcacgg
    15481 gcttcgcggg cgtgaattcg ttgccgtaga agaccgtccc ggagccgtcc tcgtgctgga
    15541 aggaccgcat atccggccag taagtgtaaa attgccatat tccgggtgga gcaaagtgcg
    15601 tgcggggtga cgtttggctc cagattccgg ccggctcgat accggagttc cagcgattca
    15661 cggagcggag cccggcccgg ccaaccgggt acggcgcagg gtcgatctcc ccgcccagac
    15721 tgacgaagtg gtggttgtac ccgttatcgg cggcgaattt gacgtagaag cgcagaaaca
    15781 ggcgcatgtg gccgggaggc aatcgccggt aaagatgccc gccttcgttc tcgcccctgg
    15841 tggcagtcat cttcagggaa cgcttgcccc tgctcgcttc cggcacgtcc ccggtccggg
    15901 cgagtgctcc gccagctttg ttgttgatgt ggctccaccg ggcgcgaaaa ccttcgatct
    15961 cctccacctc gaagtcctcg gcgaacacca cggccggatg ggtgcggatt cccgcgtcgc
    16021 cgcggtactg cgccgccagc ccgggacctt gcgggagcct ctcctgcgcc gcatttccgg
    16081 cgcaagcagg caggacgccc gcgaggaaaa ccaccagtga tctcatcaca gtccaacact
    16141 catcatgggt taaccctaac atagccgtgg atggtgcact cgcggggact gggttacggt
    16201 gcggggcgcg gcgtctatcg cgcgggccaa cccgctgcgg catgctatct ttcggggcag
    16261 gtgatcattt gagcatcgct gaattcacag ccctggtatt tgccgtctcc agctttgccg
    16321 gcttcctcgg tgcactgacc ggtttgggag gcggtgttgt gatcactccc atgctggtgc
    16381 tggcgctggg cgtagacatg cggtacgcca ttggagcgac gctggtctcc gtaatagcca
    16441 cctcctccgg cgcggcggcc gcctttgtaa aggaaggctt ctcgaatata cgggtcggaa
    16501 tgtttctcga aatcgcgacg acgctcggcg caatcgccgg cgcccaactg gcggcaaaag
    16561 cgcccgtgga ggttctcagt gtcgtcttcg ggctggtctt actctattcc gccttgattt
    16621 ccgctcgtga acaacccaag tcaggatgcg cggaagagcc ggacccgctg gcaacccgcc
    16681 tccgaatgaa tggcagttat cccaccagcg cgggtccccg ccactatgtc gttcaacgcg
    16741 tgaaagtggg tttcggaatg atgttcggag ctggaatcct atccggcctt ctcggcatcg
    16801 gttccggcgc gctgaaagtg gtggctatgg accggacgat gcgtcttcct tttaaagtat
    16861 ccacaaccac cagcaatttc atgattggcg tcaccgccgc cgccagtgcc ggcgtgtatt
    16921 tgaatcgagg ctatatcgac ccgggactga ccatgcccgt catgctgggg gtgctgctcg
    16981 ggtccgtcgc cggcgcacga attctgagcc gcacgcgtgc gcgcgcgctg cgcgtggtgt
    17041 tcggcgttgt cgttgcggtg ctggcctttc agatgatcta caacgggctc aaaggaggtc
    17101 tgtaattgtt ccgcgagtgg acggatgagg acgtcgacgg gctccttggc ggattgcttc
    17161 gagttggcgt cgtgctcgcg gccatcgttg tattggccgg cggggtggtg tacctgctcc
    17221 ggcacggaac cgaaccggca gggtacggtg tgttctacgg cgagccggac gattttcgaa
    17281 gtccttcagg aattctgcgg ggagcgcttt ctttgcgggg ccggggctta attcaattgg
    17341 gcctgcttct gttgatcgcg acgccggttg ccagggtggc attctccgtc gccgctttcg
    17401 ccattcagcg tgatcgtatg tacgttgcga tcacggtgat ggtacttctc cttctcttgt
    17461 tcagcctctt cggagcgcgg ctcccggtgt gagatccggt tatcgtccga acagcttcaa
    17521 catgcggtct cgcgcatgtt cgatatgggc tgccatcgct tcgctcgctt ccgctccgcg
    17581 ccggctgcgg agagcggcaa cgatctcgcc gtgcgggatc caatagggat cgcgccgcgt
    17641 ggacaggcaa tccagccggt gctggtattc gagcgtcagg cggagttgat ggagcacgcg
    17701 ttgattgcct gcaatctgcg cgattcccag atggaatgta tcgcttgcac ggaccacctg
    17761 cgtaatgtcg gcaccgggag tcacttccgg tgcgatcact tcctccagcg catccagttg
    17821 ctcgggcaga gccagttccg cggccttgcg tgcagagaaa cactcgagga tcttccggta
    17881 ctcataaagc tgcaacagat cggcaatcga gaccggcgta acaatatagc ctttgtgccg
    17941 cacgcgcgag agccatccat cctgcgacag ccggctgcaa gcttcgcgca ccggcgtccg
    18001 gctcgattcg cagcgccgcg cgagatccac ttcggataag acctgccccg gcgcgagttc
    18061 cccttccacc agccagctct tcaacaggcg gtatacacgc tcgagctcgc cttctttggt
    18121 caatgtgcct atggattcgg gttccaattc gatcatcata acgtctacga ttccaggatg
    18181 aagagagcat ggtggccgat ggtattcgcg cgcatacgga cagttccccg cacgacctcc
    18241 caccggaact cgctcgcatc gaccgccttc acgcttttgg gagcgccacc aagcacccgg
    18301 acaggcacct ccaccgccac cggacccacc tgcggatgcg gcaccgcttc accggagatc
    18361 accatgcccg cgtagtggtt taccaatgag acgacgtgca tcgcgtccct gcgtccgagc
    18421 acgatctgca cgcccgccgg gacctgcgcc gtcacgggca gattcgtccc gtatttctta
    18481 accgcgtaca caatgatgcg cttcagccac gtatcgtcgc gcgatgcaat ctcggagccg
    18541 atcgggcctg caatatacaa cacctgccct ttgcccacgc ggttcaatgt gacgagtggc
    18601 atacctgtgg gtttgcccca gtgtgtgtgc gaggtttcgc cgtccgtgaa cacttccggt
    18661 gtgcgatgcg cctcgggata cagcagatcc gccagcggct tcgctgtcgt gcattccact
    18721 tccagcaccg gcgtagtgaa caacacggga taggcaggcg cggaatcgcg cagctcgtcg
    18781 ccgagccagc cgtagttggc ctcgctaaac ggcgagacgc ctttaaaccg cacgccaagc
    18841 aggtcgctcc aggcaaagtt gggcaggcgc ttgccgcgcc cgtcgcgcag gctggtctcg
    18901 tgagtggcga tcagcaggcc gccgttttcg agaaagcgct cgatcgcggt gcgaagctcc
    18961 ggaggcggcg cgaacgcgtt cccgagcagt agaacgcgct gctccaccgg attcaacaag
    19021 tgcgcgggga tgacatcgaa atgcagcccg ccttccgtca aggcattgtg caggccggag
    19081 gcctccgttc gtgaaggcgt ggcgggtgtg cccggattcg gacgcaccgc caggtccgat
    19141 tgcgcatcgt agagcactgc aatacccgga acggagcgca ggccgcgcag ccacggctcc
    19201 cgttctttga ccttctccag cgtttggccg atgttgcgaa actcgtcttc gtataccttg
    19261 ccgtcaggca gcggattcac accgatgcag atgcggccgg catgcgcggt ggcaaccgcc
    19321 gcggtgacgt caagataggg cggcggaatc agattccacg ccgcccacgt tcccgtgacg
    19381 gcagacggct gcatacggat gaagccgcca taggtgtgaa tctggtagcg cttgccgagc
    19441 gactggagaa tcttggccgt gaacgaaatc aggcggtgct gcttggcctc cgacgagttg
    19501 taggtaacgc ggttgcgcaa atccacgggc gtgaagttca gctcgtcgta attgcccgct
    19561 ccgttatagc ccatctccat gccgggatcg atgcgcagca gcatatccgt caaatcgcgg
    19621 aacatccgga tgcgtccttc atgcccgaag cggttgacgc ggcgccggat ctcccagtcg
    19681 tccgaagccg cgggcatctc ttcgccgaac tgcgacttgt aagcggcgcg gcagtattcg
    19741 caaaaacacg ccgcgcccgg atcgtacgag cctaagccat atgcgttcgg catgcccaga
    19801 atgtcgaacc acatgccggg cggcttcacc gcttcctcca cctgccgcac ttcgcttaac
    19861 accacatcgc gccagggcga ccggatgcac atccagttgt aggcgcgaaa gccgatcttc
    19921 ggctggcctt tcggatcgag gcaccgccac tccggtttac gaacggcata ccagttgtcg
    19981 aggccgacat tgtagtaggc gatgaactgg atacccgctt ctttggcgaa agccgctgtt
    20041 tccgtggtcc agtcgcggtg gcacggacgg cctttgaacg ggaacatcgc atcgccgacg
    20101 gcattcttcg tcttcagctc gatgacgtcg acatgcgcct cacggcaaag ctggacgaac
    20161 tttttcgggt cgaaatactt gcccaggtcg ctggcccact cgggcatatc gaccatggtg
    20221 tgaacggcgg tgagcccgcc cgggcgggcc tccacggcca gcttccgcac gctacctttg
    20281 ctttgcgcgg atagcgaacg cgcagcggct gcggccgcgc ccgcctgtag gaaagatcgt
    20341 ctgttcatga cttaaaaaat gagtttaacc gcgaactgaa gctgcctcgt aaacaccgtt
    20401 tcgcctgtga ccgtaccgaa cgccgaactg gtgacgctgg tgttgggcgg tgtgaactcc
    20461 ggatgattga acgcgttgaa agcgtccgcg cgaatttgca ggcggactcc ctcagagaga
    20521 ttcgtgtatt tcatcagcga aaaatcccac tggtgtacgc cgtcggagcg gaccccggaa
    20581 tagtaaagcg gtccctggaa aaggtggctt ccaagctgcc gcgccggggc cgtctcaaac
    20641 acggacgtat tgaaccaccg gttcaggctc cgctcgctgc cggaaagtgg gatgtctttg
    20701 atgccaccct tgagaatgac gtctccgaaa ctgagcggag tgccgctcca ctgattcatg
    20761 atgctggaga tctgccagcc gctgatgacg gccagcaacg ggcgcggtcc attggcgagc
    20821 agcggcttgc ggcgtccgaa gggcaattcg tagagcagcg tcagcccggt atgatgtggc
    20881 cggtcgttgg gcgaaatggt ccggtacggc atggggtcgt ccgcattcag atacgtgagc
    20941 gcctccatct gtttggagaa cgtataggta agctgtgcgc tgaagccgtt ggagaacctg
    21001 cgctcggcgc ggacctgcat ggagtggtac cacgagtaac cctggtttgt cgtggtcgcc
    21061 acagaggtga actgcggaaa tgggagcaga agctggctga cggacacgtt gcgtccggcc
    21121 agcgacgtgc cgggcagcag cgggtaaaac gggttcgcca cctgggcaga gagacggtca
    21181 atcaccgcct ggtcgcgata agggagcgtg ctcagataac tgttcggcag gccgttgtag
    21241 ttcttgctga tgcgcatcgc ggtagcgcgg ctgcccgcat agcccgcctc taccaggaag
    21301 ctgccgggca gcaattgctg tatgttcagg ttccatcgct gaaggtacgg cgagttgaga
    21361 tcggaatcgt agaacgtgat cggccttccg aggaacgtca tcgcgccctg gttcgatccg
    21421 gccggcgcct gaattccgtc gggaaacgga ttgctgagcg ttgcctggta ggtgaggccg
    21481 ttgtcgagcg tggggacgat caatgtctgc tggctgtaac ccggctggaa cggacccact
    21541 cgcgaagtga taccggtatc gagatagtag aaaccgtagc cgcctcgcag caccgtcttt
    21601 tggaacgggt tataggaaaa gccgacgcgc ggcatgaagt tgcgcttctg cgcgggatac
    21661 aggccgcgag gctgccctcc ggcgcctgca aacagcagcc ctccgctgac atcgaactgg
    21721 ctggccggaa tctcgggtat agggctggcg gcatagttgg ctcgcaccgc ttcggcaatg
    21781 ggcagttgag ctattggatc gaaaccgcgc acgctgcggt tgaatcgttc cgtcatagcg
    21841 ccgagaaatt cccaacgtaa tcccatggtg agcgtcagac ggggagttac gcgccagttg
    21901 tcctgtaagt aagcgccgcc gtacgtggac tggaccgcgt agctgtcgtt gtagtccacc
    21961 attccgctat tcgggatgcc cagcaggaac gccgtgaatc cctggccgat tccggcgggt
    22021 gaagtggggc tgttgtccaa aggtccgttg gtgaagctgc tgccaaagac aaatcgcggc
    22081 gtcccgttgc cgaaatcgta gttgttcttg cggtaggtac ggaactcgcc gccgaattta
    22141 aggaagtggt tgcccgtggg ccggttcagg tcgaccatcc aggaatgaat atcgtcgccc
    22201 acctgcccgg aggtgccttc aggctggaga ccttgtagcc cggagacgtt gactccggga
    22261 aacatggttg cgacgggatg gcgatacgag agttgatcga tcacggattg ggagaatcca
    22321 agagccgaaa gatcaaatcc ctggctcggc gaccagttgc gtgcgccgaa ccgctggtat
    22381 ccgtaacgca tatcgattac cagcgtaggc gagggcgtgt acgtatcgtc cacaacaatg
    22441 ttgaacggcc gcgcacgaaa atatttgccg gtagtgatgt tccggaagta gtcccggtac
    22501 gggccttcgg ttttgaaata ttgtgcgtat cgcgcataga ggcgatgctt ctcgttcaat
    22561 acgtggtcga tgcgcgcaat gtgatcctgg tagaagtttg gcgccggcag attctgtagc
    22621 gcgtagttat tggccccgtc cgccagcccc ggaacgttag gcgagggcca gtattcggcc
    22681 atctttagcg caatcggatt gatacgatcc gccggaatga cgttgttggg aaacggctgg
    22741 cgcgtgaaac ggccgttggc cgccggcgcg gtcgtcatgg gatcgtaaat ctgatactgc
    22801 ggcccgagcc ccagcagcgc ggagaaatcg cccttgcggt ggtccaccgg cggcacactg
    22861 ccgatgaagg gataaccctg gctggcctcg tccatgatct cgtaggtgta ggcaaagaag
    22921 gtccggttgc ggccgtcgta gactttgggg atgtatacag gtccattcag gctggcgccg
    22981 ccgcgtttgt aattgaattc cgctcgaggc tggttcgcgg cattgccaaa gaacgtgttg
    23041 gcattcagcg ccggatcgcg atagaagaag tacgccgtgc catgcagtgc gttcgtaccg
    23101 gatttcgtga cgatgctgat gtgtgtaccc gacgtatggc cgacgctcgc gtcataggag
    23161 gtttcgagct tcagttcctg aatcgagtca acgggcggct gattggacat tccggaaccg
    23221 aggtcggcgt tggcggtctg cgtgtttggt acgccgtcga gggcgaattc cgtggtgccc
    23281 gccggtgctc cgttgaagct cagccagctc gacgacgcgg gccgggaggg atcctgaaat
    23341 ttcggagcgc ttgcgtaggc cccacccgcc agcgccgcgc ccgcaaattg ataaaacaag
    23401 gagcggacac tgccatgcat cgtgggcagt tcctgaaccc ggcgtgaatc cactacctgc
    23461 cctacattcg cggtggcggt ttccagcagc ggcgtttcgg ctgtgacaac cagcttctct
    23521 gtcacgtcgc caacgtccag ccggatatcc aggccgatgc gttcaccaat gcgcacctga
    23581 actcccccac gttgaaaggt tttgaagcca gggacggtgg tgctcaccga gtaattgccg
    23641 ggccgcagga acagtatctg gtaattgccc tgctcgttcg agacacccgt caccgccacg
    23701 ttcgtgctct cttcggtggc ggcaacgctg gcattgggga cggcggaacc ggaagagtct
    23761 gtcacacgtc cggtgatgct tccgcgcgac tcttgcgcgg aagcggatag agcagacagc
    23821 agggcggcta caaaaatagc cgaaagcagc ttggggaaag gcattggctc ctcctcgttc
    23881 aggtgcctgg tattgtacac ctagtgcata caacttcgtc aagcccaaaa tgatagtaga
    23941 atgaccaaaa ccgctccctt acggtcacgg ctccgaaaca gagccgcgcg cgtcagcaag
    24001 cggcgagcgc tggagtacta aggccacgca atgacgaaag ccatcgacgc acacgttcat
    24061 gtctggacag acgaccggaa gcgctatcca cgtgccaccg gcgaggccca gtaccagccg
    24121 gaccggttca cgccgcagga ttatttcgcg tactcgaagc cttcaggcat cgctcgcacc
    24181 gtgctgattc agatgagctt ctatcgcttc gacaactctt acatgctcga tacgatgcgc
    24241 gcctacccgg gcgtgttctc gggcgtcggc atcgtcgact cgtcctcgcc gcatcccgag
    24301 aagactatgc gtgacctggc gaaacacggc gtccgcggat tccggattac gcccggcgct
    24361 tcgccgca
//
LOCUS       DASTMK010000084         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_227897, whole genome shotgun sequence.
ACCESSION   DASTMK010000084 DASTMK010000000
VERSION     DASTMK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227897"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFQ79_03485"
     CDS             <1..439
                     /locus_tag="VFQ79_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEU0138741.1"
                     /translation="VNPGRAAVFVDGKYLGPAKNFGMARKYAVAAGEHEVVLREPRYA
                     EVTRRVTVEPGKTVTLKETLQPVEVAKPPFGQLKTRSNNKFDGVFVNGAYMGHVDEFN
                     NSSQALLLNPGEYTVRIVSPGGDVRQERKVTIQQDQVTLVEVN"
     gene            486..1802
                     /gene="der"
                     /locus_tag="VFQ79_03490"
     CDS             486..1802
                     /gene="der"
                     /locus_tag="VFQ79_03490"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU0138742.1"
                     /translation="MPQPSRLPSVVIVGRPNVGKSTLFNAITGQRRSIVGDEPGITRD
                     RIHGRAEYRGRPFELIDTGGIVPNEDELIPVEIFRQARVALDQADHIIFLIDGRTEIT
                     GSDRELARLLHQLGKPVSLAVNKIDSPSRETLTHEFYTLGFQDLFPVSSEHRLGLDDL
                     LERATGGFPLEREPEETEPALQRIKVAIIGRPNVGKSTLLNSLVGQDRAIVSSVAGTT
                     RDAVDESVVHGGIEYLFVDTAGIRRKGKTRLMAEKLSVVMARRHIRMANVAVLVVDAI
                     EGVTGSDATVAGYAHEEGRAVIICVNKWDAAGEKKQSAFTDDVRHELKFLEYAPVVYV
                     SAKTGKGVKKLFLLIREAYESASKRVTTGELNRFVETLRLEPDVKVKYITQASVRPPT
                     FVLFTDQRKPLHFSAERHLINQLRRQFGFSGTPIVVKTRSGRKGLS"
     gene            complement(1799..3166)
                     /locus_tag="VFQ79_03495"
     CDS             complement(1799..3166)
                     /locus_tag="VFQ79_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEU0138743.1"
                     /translation="MAEAMLPDYIARAVPNPQTNRAPWYVNTAPTYAGIFLWVVFYMK
                     LAESTIDRASIWLCLGALLVAGLLSYKLYYYVPAMLGMKTGYPLYVVGSSTFGSKGGY
                     IMPGLLMGLLQIGWFSVGTFVSTNFILNGFGVPAQPRTALFAIIAVIWGYTIAYIGAK
                     GIQYVSRVAIFLNAVPLLMVIFVFLQARGGIANYTPAKSEPFSAFALILATVIGFFAT
                     AGAAGADFGMNSRDKRDVRYGGLVGIALAILIAGGLPLLSVAGAHGLNPSLSSYSYDA
                     VIADVGGFLAPAMFFLFTIASIPSACFCAFIAGNSFSTMIPGVPRIGSMMAGVSVAIV
                     LAITGVAADLVGFFNIVGASFGPICGAMAADYMLSGWKWAGPRAGINWAGYGAWAAGF
                     IVGILPFLPLPPALAPYSQPAVLYSLIVGFIVYVVLAKAGLEPSLVPVERPEPVPVRS
                     ARAKA"
     gene            complement(3166..3810)
                     /locus_tag="VFQ79_03500"
     CDS             complement(3166..3810)
                     /locus_tag="VFQ79_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU0138744.1"
                     /translation="MISAEVCRMLEVTCHATIADVEILLRRAVQRHGAVLHTVTHLGH
                     VLNRIDNPAVKDAYVYTICQADLYAPLLEADVRFAAFLPLSIAAWTKGEGSVTLQALS
                     PLEICRILDRTSLEDLAKPVEQLLSGIMADAGEAVPAVSHAAARPKPGTRGVCPPVCL
                     VPAVSHAAARPKHSTEGATEDQVSMRAAIPQRIDRKGSKVEELAGTGVHDGSGG"
     gene            4026..>4215
                     /locus_tag="VFQ79_03505"
     CDS             4026..>4215
                     /locus_tag="VFQ79_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138745.1"
                     /translation="MQELGNAINKSLSDSERIAAAIGQIKRSGYDVFLVLEATIGFNK
                     RDEDAADDEDESFTEAQMQ"
BASE COUNT          964 a         1216 c         1251 g          784 t
ORIGIN      
        1 tgtgaatccc ggccgtgcgg ccgtttttgt agacggcaag tacctggggc cggcgaaaaa
       61 ttttgggatg gcgcgcaagt atgccgtcgc cgccggcgag catgaagtcg ttctccggga
      121 gcctcgctac gcggaagtaa ccaggcgagt caccgtcgag cccggcaaga ccgtaaccct
      181 gaaggaaacc ttacagccgg tcgaagtggc taaaccgcca ttcggccaat taaagacgcg
      241 aagcaacaac aagtttgacg gagtcttcgt gaatggcgcc tacatgggcc atgtggacga
      301 gttcaacaat tcctcacaag cgctgctgtt gaatccgggc gaatacacgg tgcgcatcgt
      361 ctcccccgga ggcgacgtcc ggcaggagag gaaagtgacg attcagcagg atcaggttac
      421 cctcgtcgag gtcaactgac acgacgaaga gtgatgtgct ccttccggta tcatggaggg
      481 agcacatgcc tcagccttca aggctgccgt cggtggtcat tgtcggcagg cccaatgtag
      541 ggaaatccac gctttttaat gcaatcaccg gccagcgccg ctcgatcgtc ggcgacgaac
      601 cggggattac gcgcgaccgt attcatggac gtgcggaata tcgcggacgg cctttcgagt
      661 tgatcgacac cggcggcatc gtgccgaacg aagacgagtt gataccggtg gagatcttcc
      721 gtcaggcaag ggtggcgctc gaccaggcgg atcacatcat tttcctcatc gacgggcgca
      781 ccgagatcac tggaagcgat cgcgaacttg ccaggctcct gcatcaactg ggcaagccgg
      841 tttcgctcgc ggtcaataag atcgacagcc cgtcgcgaga gacgctcacg cacgaattct
      901 acactctggg attccaggat ctctttcccg tttcgtccga gcaccggctc ggcttggacg
      961 accttctcga gcgtgctacc ggggggtttc ctctcgagcg ggaaccggag gaaaccgaac
     1021 cggcgcttca gcggatcaag gtcgctatca tcggacggcc caacgtcggc aaatctacgt
     1081 tgctgaatag cctggtggga caggatcggg caattgtgtc gtccgttgcc ggcacgactc
     1141 gggatgccgt ggacgaatcc gtcgtgcacg gtggtatcga gtatcttttc gtcgacactg
     1201 ccgggatccg ccgtaaaggt aagacccggc taatggccga aaagcttagc gtggtgatgg
     1261 cgcggcggca catccgaatg gccaatgtag cggtgctggt tgtggatgcc atcgagggtg
     1321 tgaccggctc cgacgccacc gtcgccggct acgcccacga agaaggccgc gcggtcatca
     1381 tttgcgtgaa caagtgggac gctgccggcg agaagaagca gtctgcgttc accgacgacg
     1441 tgcgccacga gttgaagttt ttggagtatg cgccggtcgt ctatgtttcc gccaagaccg
     1501 gcaagggtgt gaagaagctg ttcttattga tccgcgaagc gtatgagtcg gcgtccaaga
     1561 gggtgacaac cggggagttg aatcgctttg tcgagaccct tcgcctggag cccgacgtga
     1621 aagtgaagta cattacgcag gcgtcggtcc ggcctcccac atttgtactg ttcacagacc
     1681 aaagaaagcc gcttcacttt tccgcggaac ggcacctgat caaccaactg cggcgccagt
     1741 tcggcttctc cggcaccccg atcgtggtga aaacccgaag cggacggaaa ggcttaagct
     1801 aagccttcgc gcgtgcgctc cggacgggaa ccggctccgg ccgttccaca gggaccagcg
     1861 acggctccag tccggctttc gccagaacca cgtacacgat aaatccgacg atcagactgt
     1921 agagcaccgc cggctgcgaa tacggtgcca gcgccggcgg aaggggcaga aagggaagga
     1981 tcccgacaat gaaccccgcc gcccaggcgc catagcccgc ccagttgatg cccgcgcgag
     2041 gtccggccca tttccatccc gaaagcatgt aatcggcggc catggcgccg cagatcggcc
     2101 cgaatgacgc tccgacgatg ttgaagaaac ccaccagatc cgcagccacg cccgttatcg
     2161 ccaggacgat tgccacactc actcccgcca tcatcgaccc gatgcgcggg acgcccggaa
     2221 tcattgtcga gaagctgttg ccggcgatga acgcgcaaaa gcacgcggag ggaatggaag
     2281 cgatcgtgaa aagaaaaaac atggccgggg caagaaaacc gccgacgtca gctattaccg
     2341 catcgtagct gtagcttgaa agcgatggat tcaagccgtg cgcacccgcc accgaaagca
     2401 gcggcagccc gccggcaata agaatagcga gcgcgattcc taccagcccg ccatagcgga
     2461 catcacgctt gtcccggctg ttcatgccaa aatcggcgcc ggccgctccc gccgtcgcga
     2521 agaacccgat caccgttgcc agaatcaggg cgaaagcgga aaatggttcg cttttcgcgg
     2581 gcgtatagtt ggcgataccg ccgcgtgcct ggagaaacac gaatatcacc atcaacagcg
     2641 gaacggcatt cagaaaaatc gccacccgag acacatactg aattcccttt gcgccgatgt
     2701 aggcgatggt gtaaccccag atgacagcga taattgcgaa gagcgccgtt cgaggctgcg
     2761 ccgggacccc aaacccgttc aggatgaagt tggtggaaac aaatgtgccg acggagaacc
     2821 atccgatctg caacagtccc atgagcaggc caggcatgat gtatccaccc ttgcttccaa
     2881 acgtggaact tccgacgaca taaagtggat agccggtctt catgcccagc atggccggca
     2941 cgtagtaata gagcttgtag ctaagcagac cggccaccag gagcgcgccg agacaaagcc
     3001 agatgctggc cctgtcgatc gtgctctcgg ccaatttcat gtagaaaaca acccacagaa
     3061 agatgccggc ataggttggc gcggtattga cataccacgg cgcgcggttg gtttgcgggt
     3121 tcgggacagc tctggcgata taatcgggca gcatagcttc cgccatcagc ccccggagcc
     3181 gtcgtggacc ccggttccag caagttcttc gactttggag cccttgcggt cgatgcgttg
     3241 gggaatcgca gcccgcatac tgacctggtc ttctgtcgcg ccttccgtcg agtgtttcgg
     3301 gcgggcggca gcatgcgaga cggctggaac gaggcaaact ggtggacata cacctcgggt
     3361 gcctggtttc gggcgggcgg cagcatgcga gacggctgga acagcttctc cggcatcggc
     3421 catgatgccg ctgaggagct gctccacggg tttggccaga tcttccagac tggtgcggtc
     3481 gagaatccgg cagatttcaa gtggtgacag cgcctgtagc gtgacgctgc cctccccttt
     3541 cgtccaggcg gcgatgctga gtggaagaaa cgccgcgaac cgtacatccg cttccagaag
     3601 cggagcatac agatccgcct ggcagatagt gtaaacgtaa gcgtctttta ccgcgggatt
     3661 atctatccta ttcaaaacat gacccagatg ggtgactgta tgaagaaccg ctccgtggcg
     3721 ttgcaccgct cgccggagca aaatctcgac gtccgcaatc gtggcatggc aagtaacctc
     3781 gagcatgcgg cacacctccg ccgagatcat atcagagatc ctcgatcctt tccctaaaaa
     3841 gttaacagca gcctcttgaa atcctgcttc ccggccgata tatatgcctg aggtgaaagc
     3901 acatcacctt cggcgattgc tatttctgaa acgatgcagg aacatgggac atccattagg
     3961 tagagtgtta tcctgagagt ggcccaagaa aggggcaggt tttggaggaa cgactcagag
     4021 aactaatgca ggaactagga aatgcgatta acaaatcgct ttctgattct gagcgaatcg
     4081 ccgccgcgat cggacagatc aagcgctccg gctatgacgt cttccttgtc ctggaagcta
     4141 ctatcggatt taacaagcgt gatgaagacg cggccgacga tgaggacgag tccttcaccg
     4201 aggcccaaat gcaaa
//
LOCUS       DASTMK010000085         7659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_356527, whole genome shotgun sequence.
ACCESSION   DASTMK010000085 DASTMK010000000
VERSION     DASTMK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356527"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFQ79_03510"
     CDS             <1..795
                     /locus_tag="VFQ79_03510"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU0138746.1"
                     /translation="ESKGGSFDAVLAEAQRLGYAEADPTADIDGYDARSKLAILAALA
                     FGEKITPSDIFTQGIRRISPVDFDYAHRLGYTIRLVCAARQTSAGLILSVRPSLVSNS
                     TILAGVQGAYNAVWVRGAYGQDTFYYGRGAGPYPTGVAVVSDLMRVAREIRCGSPERV
                     SPFAHERLGEYQPIPVTLQASPYYLRFRVCDRPGIIAALSGILADKRISLDAVLQLPC
                     DTKHDLPFVITVEPTSEQAIREAVETMSTLDFLREPPLALPMEGSV"
     gene            792..1445
                     /locus_tag="VFQ79_03515"
     CDS             792..1445
                     /locus_tag="VFQ79_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0138747.1"
                     /translation="MRRLAVVALAVTLTLAGQVAREANERYQTREGRQQIGRMLSRPD
                     RDATQKPRELVDAMQLEPGMTVADVGTGIGYMLPYLSEAVGEKGVVLAQDIAQDFLDR
                     AKEAARHKNLTNIQYILGTTENPNLPAGQVNVVLLLDAYHHFDYPKPMLAHIGEALKP
                     GGRLVLVDYYKANFSNPGHIRLEDHEAIAEIEANGFRMISRRDHIPKSQYMAVFVKK"
     gene            1458..2027
                     /locus_tag="VFQ79_03520"
     CDS             1458..2027
                     /locus_tag="VFQ79_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138748.1"
                     /translation="MRSRALAITLAGSLPLLALVTWSSAQALPGFFDPCFLWGQQQAE
                     MTAVLEADGPCRAIRGTSQTKTQAVVNLVLVQGGILAAVLLALAGAVLTRPVLTVLGA
                     GLIFLESIPLIFSFAFLTVFASGIFLLAARQMVSFGAASRIGARVIGALALAALPLYL
                     PSMFSGALFLVFLVAALAFVAAVGWWPAA"
     gene            complement(2040..2606)
                     /locus_tag="VFQ79_03525"
     CDS             complement(2040..2606)
                     /locus_tag="VFQ79_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138749.1"
                     /translation="MPAAVMEIEHVNIKIFAKEPVEADLAGAIPMFHRWIQESVTPEL
                     LVDVADYRHVPSGPGVLLVGHEANYSLDLGGNRLGLLYNRKTAFPGDAVEKFKQAYDA
                     AVQACALIEVDPAFQGKLKFDLKHFELIVNDRLLAPNTGETWEALKPEIEKFCQSQLG
                     DAFGLRHEGGPRERFRVEIEADAPVNAQ"
     gene            complement(2606..4867)
                     /locus_tag="VFQ79_03530"
     CDS             complement(2606..4867)
                     /locus_tag="VFQ79_03530"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEU0138750.1"
                     /translation="MKASNKYWRERLVERTSPQLVKELDVYESEISLKKQGKIDDKIF
                     AETRLRRGSYGQRYDNGQRYDGRQTRRIEYPSGELTKGPSTLFDAPGMQRIKIPFGGL
                     NADQLDTLAEVAEEYSDGIAHVTTRQDIQLHFVHIEDAPAIMRRLAAVGITTREACGN
                     TVRNVTGCHLAGVCRTQTFDVTPHAKACSRFLLGHPDTQNFGRKFKISFSGCKDQACA
                     LARLNDIGCIAAVREVDGKLQRGFEVYVGGGLGTVPYQAKLFEEFVPEEEILPLAQAI
                     GLVFARLGERKNRNTARLKFLVNKLGMEEFKRLVLEERAGLPHEDAWTSYLKDIDTAG
                     EEPLKGPSLFQISGIQPESFEQWHRTNVYEQRQNGYTTITITLPLGDITATQLRKVAD
                     LARTYVKDTIRSTIEQNIVLRWVSGADVPAVYAELDSVGLAAPGAGTIVDIASCPGTD
                     TCKLGIASSRGLSGELRTRIIARGMELEEAIRGLHIKISGCFNSCGQHHVADLGFYGV
                     SRNKNGYTVPHFQVVLGGQWGENAGAYGLAIGAVPSKRIPETVDRITGRYIAERLEGE
                     SFQAFIKRIGKAECKKMIDDLSQVPPHDVDPSFYTDWADAREFTTGDLGVGECAGEVV
                     SPFEFQITACEREAFEAQLSLEAGDIDKASRLAYGAMLNGAAALLRSRMVTAGTPDEI
                     VALFRTHFYDTQLFFDPFTGGKFAHYFFHAHERAGEKRSAESVHRLIEETQLFIEACH
                     SCYGKSLQQMVAV"
     gene            5121..6275
                     /locus_tag="VFQ79_03535"
     CDS             5121..6275
                     /locus_tag="VFQ79_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138751.1"
                     /translation="MLHLAKKRGWKTAVVVEQGERPEDLNADLALPLQTVGKIRPGGT
                     APVLAVSECVWPQVKRADRSSDADADAGPTGAPWVDSNDWVSQLIRFRAPDPAIWLMY
                     APPDRVLRPEEYALGVADAAAHGARWVISGADRFTEEIWAALNRTMAFFDGHAEWQSY
                     RSLASLGVMSGFGEADEFMAHEILNLSARRNLLFRILDRSNPDLRGLRAVVWVDQDAP
                     AAQVGEKLLAFVKNGGLIVGQPVLSAMFPNARPAVEDHPSYHIYPIGTGRAAVAKEDW
                     SDPWVVAGDVHLLMSRRHDPVRFWNNGSSGSQYSVSPDGSTRLIQMVNYSARAGSDLR
                     TAGVNGKPTFARFYTPEAPQPVALVPRDARRGIELEVPAFRVYAAIELRG"
     gene            6277..7422
                     /locus_tag="VFQ79_03540"
     CDS             6277..7422
                     /locus_tag="VFQ79_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0138752.1"
                     /translation="MPTCSRRAFLSTAVAGGASFLSRAGLFAAEAPTAPVAVARCAGY
                     GRELQPVMVRMFDQLGGLEKLVKGKTVAIKLNLTGLPSHRLGHHPCEATHWTHPAVIG
                     TTIAMMGRAGARRIRLLESPWATAEPVEEYALEAGWEPRDLLNAASLVEFENTNYLGR
                     GKKYSYFPTPRGGHMFKGYLLNHSYEECDVFVSIAKLKEHATAGITLAMKNCFGNTPC
                     TIYGDGAPKDEPGLVPRGGRGPIHAGNRQPSSCAPAENDPSSPREGGYRVPRVVADLV
                     AARPIHLSIIDGIDTMTKGEGPWIRGAKFVSPRMLLAGLNPVTTDAVAMAAMGFDPMA
                     DRGTAPFETCDSTLRLAEELGVGTRDLRRIEVIGTPIRDVLFDFRKA"
BASE COUNT         1418 a         2349 c         2316 g         1576 t
ORIGIN      
        1 gagagcaagg gaggaagctt cgacgccgtt ctcgccgagg cacagcggtt gggctacgcg
       61 gaagccgacc ccaccgccga tatcgacggc tacgatgcgc gctccaagct cgcaatccta
      121 gccgcgcttg cgttcggcga aaaaataacg ccatccgata tcttcacgca aggcatccgg
      181 cgcatttccc cggtggattt cgattacgca caccggttgg gctacaccat tcgcctggtt
      241 tgcgcagccc ggcagacgtc cgcgggactt attctatcgg tgcgcccatc tctggtctcg
      301 aattccacga tcctggcggg agttcagggt gcgtacaatg ccgtctgggt gcgcggcgcc
      361 tacgggcagg acacttttta ctacgggcgc ggcgcgggcc cttacccgac cggggttgcc
      421 gtagtgagcg acctgatgcg agtggcccgc gagatccgtt gcggtagtcc cgagcgcgtg
      481 tctccgttcg cgcacgaacg gctgggcgaa taccaaccga ttccggtgac cctccaggcg
      541 agcccttatt atctgcgatt ccgcgtttgc gaccgcccgg ggattatcgc ggctctatcc
      601 ggtatactgg cggataagcg aatcagcctg gacgcggttc tccagcttcc ttgcgatacc
      661 aagcacgatc tcccattcgt tatcacggtc gagccaacct cggaacaggc gatcagagag
      721 gcggttgaga cgatgtctac actcgatttt ctgcgggagc cgccgcttgc cctgcccatg
      781 gaggggagcg tatgaggcgt ttagcggttg tggcgcttgc ggttacctta acgctcgccg
      841 gacaggtcgc gcgcgaagca aacgagcgtt accagaccag ggagggacgg caacagatcg
      901 gacgcatgct ctcccgtccc gaccgtgacg ccacccaaaa gccgcgtgaa ctcgtcgatg
      961 cgatgcaact tgagcccggc atgaccgtgg cggacgtagg caccggtatc ggatatatgc
     1021 tgccctacct gagtgaggcc gttggggaga agggagtagt gctggcgcag gatatcgccc
     1081 aggattttct cgaccgggcg aaagaagccg ccaggcacaa gaacctcaca aacatccaat
     1141 acattctggg cacgacggag aatccgaatc ttcccgccgg gcaggtgaac gtcgtcctgc
     1201 tgctcgatgc ctatcatcac ttcgattatc cgaaaccgat gctggcgcac attggcgaag
     1261 cgctgaaacc gggcggccgg ctggtgctgg tggattacta caaggcaaac ttcagcaatc
     1321 ccggtcatat ccggctggag gaccacgaag ccatcgccga gattgaagcg aatggattcc
     1381 ggatgatctc gcgccgggat cacattccga agagccaata catggcggtc ttcgtcaaaa
     1441 agtaactgta ggagcttatg cgttcccggg cgctcgcgat cacgctcgcc ggttctcttc
     1501 ctttactggc gctcgtgacc tggtcgtcgg cgcaggcttt gcccggcttc ttcgacccgt
     1561 gtttcctttg gggccagcag caagctgaaa tgacagcggt cttggaagcc gatggaccct
     1621 gccgggcgat tcgaggcacc tctcaaacaa aaacccaggc cgtagtcaac ctggttctgg
     1681 tgcagggagg aattctggct gccgttcttc tggcccttgc gggcgccgtg ctgacacggc
     1741 ccgttctcac ggttctcgga gcaggactga tctttctcga atcgatcccg ttgatcttca
     1801 gcttcgcttt cctgaccgtc tttgccagcg gcatctttct gctggccgcc cgccagatgg
     1861 tttcctttgg ggcggcatcg cggattggag cgcgtgttat tggagcgctc gcgctggcgg
     1921 cgctacccct gtatctcccg tcgatgttca gcggagcgtt gttcctggtt ttcctggtgg
     1981 cggcgttggc cttcgttgcg gccgttggtt ggtggccagc cgcttaggcg cgcgtccagc
     2041 tactgcgcat tcaccggcgc atcggcttcg atctcgacgc ggaatcgctc acgcggtccg
     2101 ccttcgtgcc gcaacccaaa agcgtcgccg agttgcgact gacagaactt ctcgatctcc
     2161 ggcttcaggg cttcccacgt ttcgccggta ttcggcgcca gcaggcggtc gttcacaatc
     2221 agctcgaaat gtttcaggtc aaacttcaat ttcccctgaa acgcgggatc gacttcgatg
     2281 agggcacagg cttgcaccgc agcatcatag gcctgtttga atttttcgac cgcatcgccc
     2341 gggaacgccg tcttgcggtt atacagcagg cccaggcgat tccctccgag atccaaactg
     2401 taattcgctt cgtggcccac cagcaaaact ccaggaccac tagggacgtg gcggtaatcc
     2461 gctacatcga cgagcagttc tggagtaacg ctctcctgta tccagcgatg gaacatcggg
     2521 atggcgccgg caagatcggc ctcgacgggc tccttcgcaa aaatcttgat attgacgtgc
     2581 tctatctcca tcaccgccgc cggcatcaga ccgccaccat ctgctgcaag ctcttgccgt
     2641 aacagctatg gcaggcctcg atgaaaagct gcgtctcttc gatgagcctg tgcaccgatt
     2701 cggcgctgcg cttttctccc gcgcgttcat gcgcgtggaa gaagtaatgc gcgaacttgc
     2761 caccggtaaa cgggtcgaag aacaactgcg tgtcgtaaaa gtgagtgcgg aacaaggcga
     2821 cgatttcatc cggagtaccg gcggtgacca tgcgggagcg cagcagcgcg gcggctccgt
     2881 tcaacatggc gccgtacgcc agccgggaag ctttgtcgat gtcgcccgct tccaggctga
     2941 gctgcgcttc gaaagcttcg cgctcgcagg cggtaatctg gaactcgaag ggcgaaacga
     3001 cctcgccggc acactcgccg actcccaggt cgcccgtggt gaactccctc gcatcggccc
     3061 aatccgtgta gaacgaaggg tccacatcgt gcggcggaac ctgcgacagg tcgtcgatca
     3121 ttttcttgca ctcggctttc ccgatgcgct tgatgaaagc ctgaaaagac tccccttcga
     3181 ggcgttccgc tatgtaacgg cccgtaatac ggtcgacggt ctccgggatt ctctttgacg
     3241 gaaccgcgcc gatcgccagc ccataagcgc cggcgttttc cccccactgg ccgcccaata
     3301 ccacctggaa atgcggcacg gtgtagccgt tcttgttccg gctgacgccg tagaaaccga
     3361 ggtcggcgac atgatgctgg ccgcaggagt taaagcatcc gctgatcttg atgtgcagac
     3421 cgcgaatcgc ttcctccaat tccatgccgc gcgcgatgat ccgcgtccga agctctcccg
     3481 aaaggccgcg ggacgaagca atgcccagct tgcaggtgtc cgtaccgggg caggaagcga
     3541 tatccacgat ggtgcccgct ccgggcgccg ccagcccaac cgagtcgagt tccgcataga
     3601 ccgccggcac gtcggctccg ctgacccatc gcaatacgat gttctgttcg atcgtactcc
     3661 ggatcgtgtc cttcacatac gtgcgtgcca ggtccgccac ctttcgcaac tgcgttgcgg
     3721 tgatgtctcc cagtggcaga gtgatagtga tggtagtata tccgttctgc cgctgctcat
     3781 agacgttcgt gcgatgccat tgttcgaagc tttccggctg gatgcccgag atctgaaata
     3841 ggcttggccc cttcaacggc tcttcaccag ccgtatcaat gtctttcagg taagacgtcc
     3901 aggcgtcctc atgcggcaaa ccggcccgtt cctccagaac aagccgcttg aactcttcca
     3961 tgccgagctt gttgacgagg aatttgagcc gtgccgtgtt gcggtttttc ctctctccca
     4021 gtcgcgcgaa caccagcccg atggcttgtg cgagcggcag gatttcctct tcgggcacga
     4081 actcctcgaa cagctttgcc tgatacggca ccgtgccgag tcctccgccg acgtagacct
     4141 cgaatccgcg ctggagcttg ccatcgactt cgcgaaccgc cgcaatgcaa ccgatgtcgt
     4201 tgagccgcgc cagagcgcac gcctggtcct tacagccgga aaacgaaatc ttgaacttgc
     4261 gtccgaaatt ttgcgtatcg ggatggccca gcagaaaacg cgaacaggcc ttcgcgtgag
     4321 gtgtgacgtc gaaggtctgg gtccggcata cgcccgcgag atggcagccg gttacgttgc
     4381 gaacggtgtt gccgcaggcc tcgcgcgtgg taattcccac ggcagccagc cggcgcatga
     4441 tcgccggtgc gtcttcgatg tgaacaaagt gaagctgaat gtcctggcgg gtggtgacat
     4501 gcgcgattcc gtccgagtac tcctcggcta cctccgccag cgtgtccaac tggtcggcgt
     4561 tgagcccgcc gaaggggatc ttgatccgct gcatgccggg cgcatcgaac agggtggagg
     4621 ggcctttcgt caactcgccg gaggggtatt cgattcgccg cgtctgtcgc ccgtcgtagc
     4681 gctgtccatt gtcatagcgt tgcccgtagg agccgcggcg cagacgtgtc tcggcgaaaa
     4741 tcttatcgtc gatcttccct tgcttcttca gcgaaatttc cgattcgtag acgtcgagtt
     4801 ccttgacgag ctgaggcgac gtcctctcga cgagcctttc tctccaatac ttgttgctgg
     4861 ctttcattta tcggctccga ttattcaccc tattccctat agacattacc atatttgccg
     4921 ccttcttagc aacatccgat actcggaatc ccggttgagc ttgctatgat tatcgcagca
     4981 tgccagcccc ggacacgtgg aaacattcct ggcccctgct gtggccggaa tccttgaacg
     5041 aaggtcatct cgatcaagtc ggcaagacac caattaattg tctcttattc tctcccgcgc
     5101 aggcccaacg gcacaaaagc gtgctccact tagccaaaaa acggggatgg aaaacggcgg
     5161 tcgtcgtcga gcagggagag cgccccgagg acctcaacgc ggacctggcg cttcccctac
     5221 agacggtcgg aaagattcgg cctggcggaa cggctccggt tctcgctgta agcgagtgtg
     5281 tgtggccgca ggtgaagcgg gcggacagaa gctcggatgc ggatgccgat gccggcccca
     5341 cgggggcgcc gtgggtggac tcgaacgact gggtttctca gctcatccgc tttcgcgctc
     5401 cggacccggc aatctggctg atgtacgccc cgcccgatag agtgcttcgg ccggaggaat
     5461 atgcgctggg agtcgcggat gcggccgctc acggcgcgcg atgggtgata tccggagcag
     5521 accggttcac ggaggagatc tgggccgcac ttaatagaac gatggcgttt ttcgacgggc
     5581 acgccgaatg gcagagctat cgctccctgg cgtcgctggg agtgatgtcc gggttcggag
     5641 aggccgacga gttcatggcc cacgagatcc tgaacctcag cgcacggcga aacctgctgt
     5701 ttcggattct cgaccggagc aatccggatc tgcgcggatt gcgcgcggta gtttgggtgg
     5761 accaggatgc acccgccgca caggttgggg agaaactgct ggcctttgtg aaaaacggcg
     5821 ggttgatcgt tggtcagccg gtattgtcag ccatgttccc caatgcacgg ccagcggtgg
     5881 aagaccaccc cagctatcac atttacccga ttggcacggg ccgcgccgcc gtggcaaaag
     5941 aagactggtc cgacccctgg gtggttgccg gcgacgtaca ccttctgatg agccggcgcc
     6001 acgatccggt tcgcttctgg aataacggat cgagcggatc gcagtactct gtgtctccgg
     6061 atggaagcac gcgattgata cagatggtga attattccgc ccgcgcgggt tcggatctta
     6121 ggacggccgg cgtgaacggc aagcccacct tcgcacgctt ttacacgccc gaggcgcctc
     6181 agccggttgc gctggtcccc agagacgccc ggcgcggcat cgaactcgaa gtacctgctt
     6241 tccgtgtata tgctgcaatc gaattgaggg gttgacatgc caacttgtag ccgtcgtgcg
     6301 tttctgtcca ctgccgttgc gggtggagct tcgtttctat cccgcgccgg gctcttcgcg
     6361 gccgaggcgc ctacggcacc ggtggctgtc gcccgctgcg ccggatatgg ccgcgaactc
     6421 cagccggtga tggtgcggat gttcgatcaa ctgggcgggt tggaaaaact ggtgaagggc
     6481 aagaccgtcg ccatcaagct caacctgacg ggcctgcctt ctcatcgctt ggggcaccac
     6541 ccgtgcgagg ccacacactg gacacacccg gcggtgattg gcacaactat agccatgatg
     6601 ggccgcgccg gggcgcgccg catccggctg ctggagagtc cctgggcgac ggcggaaccg
     6661 gtggaggagt atgcactgga ggctggatgg gagccgcggg atctgttgaa tgccgcctcc
     6721 ctggtggaat tcgaaaacac gaactacctc gggcggggca agaaatactc gtactttcca
     6781 accccccgcg gtggccacat gttcaagggc tacttactga accactctta tgaagagtgc
     6841 gacgtcttcg tctcgatcgc caagctgaag gagcatgcaa ccgcgggcat cacgctcgcc
     6901 atgaagaact gcttcggcaa tacaccctgc acgatttatg gtgacggcgc tccgaaggac
     6961 gaaccgggcc tggtgccgcg aggcggccgc ggccccattc atgccggcaa ccggcaaccc
     7021 tccagttgcg caccggctga gaacgacccg tcgtcgccgc gcgagggcgg ctaccgtgtg
     7081 ccccgcgtag tcgccgacct tgtcgcggcc cggcccatac acctgtcgat catcgacggc
     7141 atcgatacga tgaccaaggg cgaagggccg tggattcgcg gcgcgaaatt tgtcagcccg
     7201 cggatgctgc ttgctggcct gaatccggtt acgacggacg ctgtggcgat ggcggcgatg
     7261 ggcttcgacc cgatggccga ccgcggtaca gcgccgtttg agacctgcga cagcaccctc
     7321 cggttagccg aagaactggg cgtgggaact cgcgacctgc gccgcatcga agtgatcggc
     7381 acacccatcc gcgatgttct gttcgatttc cggaaggctt agtgcttcta tccgataccg
     7441 cttgctcgcg cgcgcggctc cgtttccgag ccgtgaccgt gagggagcgg ttgtcctggt
     7501 tcgacttccg gaaggcttag ctgacacgcg ttaccgagcc ttgaccagcc gttgtgccag
     7561 ttcggcgcgg gtgaccgggc cgttgcgttt cgtatccatc ggaggcaggg tgaaaggcag
     7621 tttctcttcc ctccagtcgg tccagagaaa gggcaggtc
//
LOCUS       DASTMK010000086        11108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_292482, whole genome shotgun sequence.
ACCESSION   DASTMK010000086 DASTMK010000000
VERSION     DASTMK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292482"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="rpoD"
                     /locus_tag="VFQ79_03545"
     CDS             <1..742
                     /gene="rpoD"
                     /locus_tag="VFQ79_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU0138753.1"
                     /translation="EQEAERAKKDLVEANLRLVVSVAKKYVNRGLHLLDLIQEGNIGL
                     MRAADKFEYRRGFKFSTYATWWIRQAITRAIADQSRTIRIPVHMNESMNKFLRATREL
                     EKEMGRVPTNEEISRRMDIPVEKVQKLKTISRDPVSLETPVGRDGESALGDLIEDRWV
                     GSPVDAVIDSNVKDETANILKTLSPKEEKVIRLRFGIGCEREHTLEEIGQEFDVTRER
                     IRQIEAKALRQLRSPERARHLRALLAAR"
     gene            905..1870
                     /locus_tag="VFQ79_03550"
     CDS             905..1870
                     /locus_tag="VFQ79_03550"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU0138754.1"
                     /translation="MPEAGKRILLLTPRGFCAGVVRAIDVVKIALDLFGPPIYVRKEI
                     VHNRHVVDELRSAGAVFVEELSQVPSGARVIFSAHGVSPAVRAEAEKRGLQVIDATCP
                     LVTKVHLEAVKFARLGYTIVLIGHKDHDEVIGTLGEAPESTRLVSTVEDVDQLEVADP
                     ARVCYLTQTTLSLDETRDVVARLKEKFPEIHGPPAQDICYATENRQMAVKAVVSTCDL
                     LLVVGSQNSSNSRRLVEVCQREGVPAYLIDDAAEAHPAWFEEARTVAVTAGASAPENL
                     VSSLIESLKVQGFHTVEEINIKDEDVRFSLPAGLEAAASRLTIIA"
     gene            1867..3810
                     /gene="shc"
                     /locus_tag="VFQ79_03555"
     CDS             1867..3810
                     /gene="shc"
                     /locus_tag="VFQ79_03555"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEU0138755.1"
                     /translation="MSTTGIRLRESVSGAASRCVRRAADALLELQNPQGYWWGELTAD
                     STLESDYILLQLWLYPPDGDGVWRPPNRQQIDRAVRSILKRQLPGGGFNIYEKGPADL
                     SATVKAYFALKLAGVPADDPALRQAREVILSLGGIQAANSYVKLNLSLFGLYPREHCP
                     SIPPEMLLLPANFIYRMSSWTRAIVIPLSIVHAADPRRPVPAGFDLRELFLEGVSPAF
                     RRSARLFSWRNFFLGIDRGLKWWERHGSRRVRARAVKRAEEWMLERTRFSDGLGAIYP
                     PMMYLIMALDVLGYPPEHPERQEAQRQFDNLMVDEGERFFFQPCFSPVWDTGIALQAL
                     EESGCVPEEPLAQAADWLLTKEVRRKGDWSVKRPNLEPSGWYFEFANEFYPDIDDTAQ
                     VLLGLRHARARDAAAQQACERRAVEWLLGMQSKDGGWAAFDVDNNWTPLSYVPFADHN
                     AMLDSTCPDITGRVLEALCVYGLDSASPAVRRGVEYLQRTQEADGSWYGRWGVNYIYG
                     TFLALRGLQMAGESNREAYILRAGEWLRSIQNADGGWGEDCASYEQGSFVQAHSTPSQ
                     TAWAILGLLAGGDGDSLSVRNGIEYLIDTQRPDGTWEEDLSTGTGFPRVFYLAYHLYR
                     NSFPLLALSVYVNSHNAAGGAGK"
     gene            3807..4919
                     /gene="hpnH"
                     /locus_tag="VFQ79_03560"
     CDS             3807..4919
                     /gene="hpnH"
                     /locus_tag="VFQ79_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEU0138756.1"
                     /translation="MRFPLSMTAGMAGYIARNKLRPKPEWQLNRKNGTDVSSPFRIIH
                     SSVERTRPPERHPMIGKRFPLVLMLEPLHACNLTCTGCGRIREYETSITEKLSVEQCL
                     AASDECGAPIVSICGGEPLLYPEIGRLSAEILKRGRHIYLCTNGMMIKKRLAEFQPDG
                     HFFFNVHLDGMEKTHDIAVEREGVFRQAIEGIRVAKQAGFKVCTNTTIYKETDMKEIE
                     ELFEYLEQFEVDGHMLSPAYGYSAVNDREIFMTRDDVHEKFRDIDRMAKRFRLNSTPV
                     YLDFLKGERELPCTAWGNPTYNIKGWKGPCYLITDAHHATFEDLMTKTPWENYGSGND
                     PRCEHCMTHCGYEPSAALGINAKFSDNFRLLSWALR"
     gene            5035..6009
                     /gene="hpnA"
                     /locus_tag="VFQ79_03565"
     CDS             5035..6009
                     /gene="hpnA"
                     /locus_tag="VFQ79_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEU0138757.1"
                     /translation="MKPALVTGASGFVGWHVARRLLDRGYSVRALVRPGSKVRDLDVE
                     TVAGDLREPESLNRAASGCGVIFHVAADYRLWARRPQELYLSNVDGTANLLEAARKQG
                     VECVVYTSTVGCIGVPEGGIGDERQPVTLEEMTGTYKRSKFLAEKRAREFAESGFPVV
                     IVNPTTPVGDHDFKPTPTGRIVLDFIRGAMPAYVDTGLNFVDVRDTAEGHLLALERGR
                     TGERYILGAENLSLQELFARLERLSGVRAPSVRIPYFIAYAAGMASTGLAQITGLEPR
                     APLDGVRMSRKKMFVSHEKAGRELGYAPGPVDGALARAVEWFRSNGYC"
     gene            6011..6715
                     /locus_tag="VFQ79_03570"
     CDS             6011..6715
                     /locus_tag="VFQ79_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138758.1"
                     /translation="MRNILVIAAEHSELSGLLRHLGKVERLRWPLDYAAVGERSGERW
                     WLLANGAGRTLAGGAADAVHGKQRFDSILSVGLCGGVDPALTAGEVVVASEILDIAGA
                     KRLAGTQPATARPFACGCLATVDSVIRTTSDKARIRAMGAVAVDMEAAAVAGRAANWG
                     IPFYCVRAVSDTAGEAIQLDLNACRSKNGRIRRMRVLAAAMQNPRRLLPELIHLWRRS
                     RQAAGALGDFLADCRF"
     gene            6813..7793
                     /locus_tag="VFQ79_03575"
     CDS             6813..7793
                     /locus_tag="VFQ79_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0138759.1"
                     /translation="MLVQVESCGICHTDLKKIEYNLLPPPRIYGHETAGTVVAVGEGV
                     RAFSIGDRVVVFHHVPCGNCFYCRKKLYAQCEVYKRVGITAGFEPAGGGFSQYVRVMD
                     WIVERGVEKIPDGVSFDTACLVEPVNTCLKAVVQADPQRDETVLILGQGPIGLLFTML
                     VHRLGSTVVATDTISARRELSVRAGAAECWDPRAVDLRDKIFNITENRGADLVIVAAS
                     APELVRDAIACSRPGSRILFFAQTSDKERIEVSGADICVGERVLFGSYSASVDLQRQS
                     AELVFSGELPVEDLISHRIPLNQISEGFHLALHPDAKSLKIIVQPQRWSE"
     gene            7790..8830
                     /locus_tag="VFQ79_03580"
     CDS             7790..8830
                     /locus_tag="VFQ79_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0138760.1"
                     /translation="MKTQMMAAVLYGKEHLQVEEVAMPEVQSGDVLVRVRAALTCGTD
                     VKVFRRGYHARMIVPPALFGHELAGDVVAVGAGVSRFRPGDRVVAANSAPCLECFYCR
                     RGLENLCEDLLFNNGAYAEYIRIPGRIVERNTYEIPPHISYQDAALIEPLACVLRGLE
                     ETGLRAGDTVAVIGLGPIGLMFVRLAKVFGARVIAFGRRPTQLERAIRMGAEEAISTA
                     TDPNPVKTAKSLTDGRGVDIAIEAVGKPETWEMAVGMVRRGGCVNFFGGCPNESRVTL
                     DTSTLHYSEITCRASFHHTPMHVRAALDAVARGDIRARDFVNGEEPLTNLLEVMRHLM
                     SHNGHLKTAILP"
     gene            8838..9758
                     /gene="hpnC"
                     /locus_tag="VFQ79_03585"
     CDS             8838..9758
                     /gene="hpnC"
                     /locus_tag="VFQ79_03585"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEU0138761.1"
                     /translation="MSAFLPPRDFVASPDSMDRTWTDADSLAYTRWLATSHYENFHVV
                     SFLLPKRLHQDFYNVYAFCRWADDLGDEIGDRAESLRLLSWWRGELSALYDGQAAHPV
                     FVALRTTVKRHDLPSDPFSDLITAFELDQTKARYENWDELFYYCRYSANPVGRLVLHL
                     CGYHDAGRQALSDATCTALQLANHWQDVTVDLAKDRLYLPLELLAKHACSVEDIFARQ
                     FTPAFAAAMKEAADVARRLFTTGLPLARTVNRRLAIDLELFSRGGMRILDKIERQGYN
                     VLAQRPYVSKAEKVRLLFGAVARAVFLRAA"
     gene            9755..10663
                     /locus_tag="VFQ79_03590"
     CDS             9755..10663
                     /locus_tag="VFQ79_03590"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEU0138762.1"
                     /translation="MTQTLAQSYRYCRRVARTRAKNFYYSFLLLSRTQRDAMCAVYAY
                     MRQCDDLSDDPAHGSRKTARASLERWRVDLDRALHGDYGENPLWPALDDSVRRYGIPP
                     QYLYEMIDGVTSDLEPRRVETFEELYQYCYQVASVVGLTVIHILGFHSPAALPLAEKC
                     GVAFQLTNILRDVREDAERDRIYLPAEDLASFGVDPAALAKGDKTGAFARLMEFEANR
                     AFRYYHDSLPLVPMVHNSSRASLEALIRIYYRLLERIRDSDYDVLHRRIRLAPWEKMA
                     ILAGTLLGRRDRFWQKHAAGGSRAED"
     gene            10670..>11108
                     /locus_tag="VFQ79_03595"
     CDS             10670..>11108
                     /locus_tag="VFQ79_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0138763.1"
                     /translation="MITAVSRREFLRTASAGAFAPALRSSKPNILFLLTDDQRWDALG
                     CMGRPVIETPHIDRLAREGVTFENNFCASAICMTSRASIFTGLHERTHGISSFTQDFT
                     PEQLSATYPQILREAGYRTGFIGKYGVGNNLPAAHFDYFKGFPG"
BASE COUNT         2184 a         3089 c         3543 g         2292 t
ORIGIN      
        1 cgagcaggaa gccgaacggg cgaagaaaga ccttgtcgaa gccaaccttc gcctggtggt
       61 ctcggtggcg aagaagtacg tgaaccgggg tttgcatctc ctcgatctga tccaggaagg
      121 caatatcggt ctcatgcgcg ccgccgacaa gttcgagtat cgccgcggct tcaagttctc
      181 cacgtatgca acgtggtgga tacggcaggc gatcacccgc gccattgcgg accagtccag
      241 aaccattcga atcccggtgc acatgaacga gagcatgaac aagtttctgc gtgccacacg
      301 ggagctcgaa aaagagatgg gccgggtgcc gaccaatgag gagatcagcc ggcgcatgga
      361 catcccggtg gaaaaggttc agaagttgaa aaccatttcc cgcgatcctg tttcgctcga
      421 gaccccggtg ggccgggacg gcgaatccgc ccttggcgat ctgattgaag accggtgggt
      481 tggttcgccc gtggacgcag tcatcgattc caacgtaaaa gacgaaacag cgaacattct
      541 gaaaacgttg tctcccaaag aggagaaggt gattcgtctc cgcttcggga tcggatgcga
      601 acgggagcac acgctcgaag aaatcggtca ggagtttgac gtgacgcggg agcggattcg
      661 tcagattgaa gccaaggctc tacggcaatt gcgttctcca gagcgggcgc gtcatctccg
      721 tgcgttgctc gcagcccggt aacgccggga ttataaatcg gatttaatcg acaaaggccg
      781 gtatgggaaa ccggcctttt tgtccatcgg gcgtcccccc gcctgctgcc accgctcaat
      841 tcgaaccgtt taattgtgaa ttggagggcc ggtgcacggc gattgctata atgaagagtt
      901 ccgtatgccg gaagctggga aaagaattct attgctcacc cctcggggct tctgcgcggg
      961 tgtcgtgcgg gcgatcgatg tagtgaaaat cgcgctcgac cttttcggcc cgcctattta
     1021 tgtacgcaaa gagatcgttc ataaccggca cgtcgtggac gaattgcgta gcgccggggc
     1081 ggtttttgtg gaggagttga gccaggtgcc gtccggtgca cgcgttattt tcagtgcgca
     1141 cggcgtttct cccgcagttc gagcggaagc cgaaaagagg gggcttcagg tcatcgacgc
     1201 cacttgcccg ctggtgacca aggtacacct cgaagccgtc aagttcgccc ggcttggcta
     1261 cactatcgtt ctcatcggac acaaggacca tgacgaggtg attggcacac ttggcgaagc
     1321 accggaatct acacggctgg tctcgaccgt cgaagacgtg gaccaactcg aggtcgcgga
     1381 tccggcgcgc gtgtgctacc tcacacagac gacgctcagc ctggatgaga ccagggacgt
     1441 ggtagcgcgg ctgaaggaga aattcccgga aattcatggt cctcccgcac aggatatctg
     1501 ttatgccacc gagaaccgcc agatggcggt gaaggctgtc gtctccacat gcgatctgct
     1561 tctggtagtt ggatcccaga acagttcgaa ctcaaggcgc ctggtggaag tctgccagcg
     1621 ggaaggagtg cctgcttatt tgatcgatga tgccgccgaa gcccaccccg cgtggttcga
     1681 agaagcccgg acggttgcgg taaccgcggg agcgtccgcg ccggagaatc ttgtttcatc
     1741 gctgattgaa tccctgaagg ttcaagggtt tcatacggta gaggagatca atattaaaga
     1801 cgaggatgtc cgtttttcgc tccctgccgg gttggaagcg gcggcgtccc gcctgacgat
     1861 aatcgcatga gcaccaccgg gatccgcctg agggagtctg tttccggggc tgcatcgcgg
     1921 tgtgtccggc gagcggcgga cgcgctgctg gaactgcaaa atccgcaagg ctactggtgg
     1981 ggagaactca ccgcggattc cactctcgaa tccgattaca tcctgcttca gctttggctc
     2041 tatcctccgg atggcgacgg cgtctggcgc cctcccaaca ggcagcaaat tgatcgcgcc
     2101 gtacgctcga tcctgaaacg gcaattaccg ggtggcggat tcaatattta cgaaaagggt
     2161 cccgccgatc tgagtgcgac ggtcaaagct tactttgcgc tcaagctggc tggtgtcccg
     2221 gcagacgatc cggctttgcg ccaggcgcgc gaggtcatcc tgtcgctggg cggcatccag
     2281 gcggccaaca gctatgtcaa gctgaacctg agcctgttcg gcctttaccc acgggagcac
     2341 tgtccgtcaa ttcctcccga aatgcttctg ctccctgcaa atttcatcta ccgcatgtcc
     2401 tcgtggaccc gggccatcgt gattccgctg tccatcgtac acgcggccga tccgaggcgg
     2461 ccggtcccgg ccgggttcga tctcagggag ttgttcctgg agggcgtcag ccccgcgttc
     2521 cggcgcagcg cgaggctctt cagttggcgg aactttttcc tggggataga caggggattg
     2581 aagtggtggg agcgccacgg ttcgcggagg gtgcgcgcgc gggcggtgaa acgggcggaa
     2641 gaatggatgt tggagcgcac ccgtttcagt gacggccttg gcgcgattta cccgcccatg
     2701 atgtacctga ttatggcgct ggatgtgctc ggctacccgc cggaacatcc ggaacgccag
     2761 gaggcgcagc ggcagttcga taatctgatg gtggatgagg gcgaacggtt cttctttcag
     2821 ccttgttttt cgcctgtgtg ggacaccggc atcgcgctgc aggcgctgga ggagtccggc
     2881 tgcgtaccgg aagagccgtt agcccaggcg gcggactggc tcctcaccaa agaagtccgg
     2941 cggaaagggg actggagcgt gaagcgcccg aacctggagc cgtcgggctg gtacttcgaa
     3001 tttgccaacg aattttatcc cgacatcgac gacacggcgc aggtgctgct cgggttgagg
     3061 catgcgcgcg ccagagatgc cgcggcgcag caggcttgtg aacgccgggc cgtggagtgg
     3121 ctgctgggta tgcagtccaa ggacggggga tgggctgcct tcgacgtcga taacaactgg
     3181 acaccgctca gctatgtgcc gttcgccgat cacaacgcca tgctcgattc cacctgtccg
     3241 gatatcaccg ggcgcgtgct ggaggcgctt tgtgtctatg gtctggacag cgcttccccg
     3301 gcggtccggc ggggagtcga atacctccag cgcacacagg aggcggacgg gagttggtac
     3361 ggccggtggg gtgtgaacta catctacgga acgttcctgg cccttcgcgg tttgcagatg
     3421 gcgggagaga gcaaccgtga ggcctatatc ctgagggcgg gcgaatggct gcgatccatt
     3481 cagaacgccg atggaggctg gggcgaggac tgcgcgagct acgaacaggg ctcgtttgtc
     3541 caggcccaca gcactccttc ccaaaccgcg tgggccattc tgggattact cgccggaggc
     3601 gacggcgata gcctcagcgt gcgaaatgga atcgagtatc tgatagacac acagcggccg
     3661 gacggtacgt gggaggaaga tctgtccacg ggcaccggct tcccgcgagt gttctatctt
     3721 gcttaccacc tgtatcgcaa ttcgttcccg ctgctggctc tgtccgtata cgtcaactcg
     3781 cacaacgccg ccggaggggc tggcaaatga gatttccgct atcgatgacc gctggtatgg
     3841 ccgggtatat cgcccgcaat aaactgcggc cgaaaccgga gtggcagcta aaccggaaga
     3901 acgggaccga tgtttcgagc cctttccgga tcatacacag ttccgtggaa cggacgcggc
     3961 cgccggagcg gcacccgatg atcgggaagc ggtttcccct ggtgctgatg ctggagccgc
     4021 tgcatgcgtg caatttgacc tgcacgggtt gcgggcgcat ccgcgaatat gaaaccagca
     4081 tcaccgagaa gctatcggtg gaacagtgcc tggcggcatc ggatgagtgt ggggcgccga
     4141 tcgtttccat ctgtggagga gaaccgctgc tctatcccga gatcggccgt ctttcggcgg
     4201 agattctgaa gcgtggccgc cacatttatc tctgcacgaa cggcatgatg atcaaaaagc
     4261 ggctggccga gtttcagccc gatggccact tcttttttaa cgtgcacctg gatggtatgg
     4321 aaaagacgca cgacatcgcg gtggagcgcg aaggcgtttt tcgccaggcg atagagggca
     4381 ttcgcgtggc caaacaggcg gggttcaaag tctgcaccaa cacgaccatc tataaagaaa
     4441 ccgacatgaa ggagatcgag gagttgttcg aatatctgga gcagttcgag gtggacggcc
     4501 atatgctctc ccccgcttac ggatattcgg cggtgaacga tcgtgagatc ttcatgacgc
     4561 gcgacgacgt gcacgaaaaa ttccgggaca tcgatcgcat ggccaaacgc ttccggctga
     4621 acagcacccc ggtgtacctg gattttctca agggcgagcg ggaactgccg tgcacagcct
     4681 gggggaaccc gacctacaac atcaagggct ggaaaggccc atgctatctc atcacggacg
     4741 cgcatcacgc tacattcgaa gacctgatga cgaaaacacc gtgggagaat tacggcagtg
     4801 ggaacgatcc gcggtgcgag cattgcatga cgcactgcgg gtatgagccg tcggcggcgc
     4861 tcggaatcaa cgcgaaattc agtgacaact tcaggctgct ctcctgggcg ctgcgatagg
     4921 atcttctttg agcctccttt ttcgcgccac cgccgtaccc ggtcctgccg caatgaaccc
     4981 gcaaccatgg tgtcctgcgc ggattgagcc tcaccacggg cttgcggggc agcagtgaag
     5041 ccggcgttgg tgacgggcgc ttcggggttt gtcggctggc atgttgccag gcggctgtta
     5101 gaccgtggtt actctgttcg cgcgctcgtt cgccccggca gcaaggtacg cgacctggac
     5161 gtcgaaacgg tagccggcga tctccgcgaa cccgaatctc tgaatcgggc ggcgtcaggc
     5221 tgtggcgtca tttttcatgt ggcggccgat taccgcctgt gggcgcggcg cccgcaggaa
     5281 ctatatctgt ccaacgtaga tggcacggcg aatctgctcg aagccgcccg gaaacagggg
     5341 gtggaatgtg ttgtttatac gagcaccgta ggttgtatcg gtgtgccgga aggcggcatc
     5401 ggcgatgagc ggcagccggt gacgctcgag gaaatgacgg gcacgtacaa gcgatcgaaa
     5461 ttcctggctg aaaaacgggc acgcgagttt gccgagtcgg gttttcccgt ggtgatcgtg
     5521 aacccgacca cgcccgtcgg agatcatgat ttcaagccca cgccgaccgg ccgcatcgtg
     5581 ctcgatttca tccgtggcgc catgccggcc tacgtggata ccggattgaa ttttgtcgac
     5641 gtgagagata ccgcggaggg ccatctgctt gcactggagc gcggcaggac aggggaacgg
     5701 tatattttag gcgcggagaa cctctctttg caggagctat tcgcgcgcct ggagcgcctc
     5761 agcggagtgc gcgctccttc cgtccggatt ccctacttca tcgcgtatgc cgccggaatg
     5821 gccagtacag gtctggctca gattaccggc cttgagccca gggcaccgct cgacggcgtt
     5881 cgcatgtcac gcaaaaaaat gttcgtctcg cacgagaagg ctgggcggga actgggctat
     5941 gcgcccggtc cggtggatgg agcactggcg cgtgcggtcg agtggttccg ttcgaatggt
     6001 tattgttgag gtgcggaaca ttttagtcat tgcggccgaa cacagcgaac tatcgggctt
     6061 gctgcgccat ctcggaaagg tggagcggct gcgctggccg ctcgactacg ccgcggtggg
     6121 cgaaaggtcc ggcgagcgct ggtggctgct ggcaaacgga gcgggacgca ccctggccgg
     6181 cggagcggcg gatgccgtgc acgggaagca gcggtttgat tccattctca gtgttggact
     6241 gtgcggcggg gtcgaccccg cgctgaccgc cggagaagtg gttgtcgcca gcgagattct
     6301 cgacatcgcc ggcgcaaaga gactggctgg aacgcagccc gcgacggccc gcccctttgc
     6361 ttgcgggtgt ttggccacgg tggacagcgt gatacggact acgtcggaca aggctcgtat
     6421 tcgcgcgatg ggagcggtag cggtggacat ggaggcggca gccgtcgcgg gccgtgccgc
     6481 gaattggggg attccgtttt attgcgtacg agcggtttcc gataccgcgg gcgaagcgat
     6541 tcaactggat ttgaacgctt gccggagtaa gaatggacga attcgccgca tgcgcgtcct
     6601 ggccgccgcg atgcagaatc caaggcggtt gttgccggaa ttgattcatc tgtggcgccg
     6661 tagcaggcag gcggccggcg cattggggga ctttcttgca gactgccggt tttgatggct
     6721 tcattcctac cgggatgcgg gcggcggtgt atcgcggttc gagcacagtg tcagttgagg
     6781 agatacctac tcccggaata gggcgtggag aaatgctcgt ccaggtcgaa agctgcggga
     6841 tctgccacac cgacctgaag aaaattgaat acaacctttt acctccccca agaatctatg
     6901 gtcatgagac cgcaggtacc gttgtagcgg ttggggaagg cgttcgcgcg ttctccattg
     6961 gcgaccgtgt ggtggtgttc caccatgtcc cctgcgggaa ctgcttctac tgccgcaaaa
     7021 agctgtatgc gcagtgcgaa gtgtataaga gagttggaat tacagccgga ttcgagcctg
     7081 ccggcggtgg attttcccaa tacgtgcgag tgatggactg gattgtcgag cgcggtgtcg
     7141 aaaagatccc ggatggggtc tccttcgata cggcctgcct tgtggagccg gtaaacacgt
     7201 gtctgaaggc ggtggtgcag gccgatccac agcgggacga aacagtgctg atcctcggcc
     7261 agggtccgat cggcttgctg tttacgatgc tggtgcatcg cctggggtcg accgtcgtcg
     7321 cgacagatac cataagcgcg cggcgggaat tgtccgtccg tgcgggcgcg gcggagtgtt
     7381 gggacccgcg agccgtcgac ttacgtgata aaattttcaa catcaccgaa aacagaggag
     7441 cggaccttgt tatcgtagcg gcctccgctc cggaactggt gcgcgatgcg atagcgtgtt
     7501 cgcggccggg aagccgtatt ttgtttttcg cacagacttc ggacaaggag cggattgagg
     7561 tgtccggtgc ggatatctgt gtgggagagc gggtattatt tgggtcttat agcgcttcag
     7621 ttgacctgca acggcagtcc gcggaacttg tatttagcgg cgagctgcct gtcgaagatc
     7681 ttatttccca ccggattccg cttaatcaaa tctcggaagg ttttcacctg gcattgcatc
     7741 ccgacgctaa atccttgaag ataatagttc aaccacagag gtggtcagag tgaagacaca
     7801 gatgatggct gccgtgctct acggcaagga gcatttgcag gtagaagagg tagccatgcc
     7861 agaggtccaa agcggtgatg tgctcgttcg cgtcagagcg gcgcttacct gcgggacaga
     7921 cgtgaaggta tttcgccgcg gctatcatgc gcgcatgatt gtaccgccgg cccttttcgg
     7981 gcacgaatta gcgggggatg tggtcgctgt aggcgccggg gtcagccggt tcaggccggg
     8041 cgaccgggtc gtggcggcca attccgctcc ctgtctcgag tgtttctatt gccggcgcgg
     8101 actggaaaat ctttgcgaag atctgctttt caataacggg gcgtatgcgg aatacatccg
     8161 gattccgggg cgtattgtag agcgaaatac ctatgaaatt ccgccccaca tcagttatca
     8221 ggacgcggcg ctaattgagc ctctcgcctg tgttttgcgg ggcctggaag aaacagggct
     8281 gcgcgcggga gacaccgtag cggttatcgg attgggcccg atcggtctga tgttcgtgcg
     8341 tttggcgaag gttttcggtg cgcgggtgat tgcctttgga cggcggccca cgcaactgga
     8401 aagggccatc agaatgggcg ccgaggaggc gatttcgacc gccaccgatc caaaccccgt
     8461 aaaaacggcg aagagcctga cggacgggcg cggtgtggat atcgcgatcg aagcggtcgg
     8521 caaaccggaa acatgggaaa tggcggtagg aatggtccgc cgcggcgggt gtgtgaactt
     8581 tttcggaggt tgcccgaatg aaagccgggt gacgctcgac acctcgacgc tgcactattc
     8641 cgaaatcacg tgccgggcca gtttccacca cacgccgatg cacgtgcgcg cggcgctgga
     8701 tgcggtggct cgcggcgaca ttcgtgcgcg ggattttgtc aacggcgaag agccactgac
     8761 caatctgctg gaagtgatgc ggcatctgat gagccacaac ggccatttga aaaccgccat
     8821 tctgccgtaa ccggatcttg agcgcatttc tcccaccgcg ggatttcgtt gcatctccgg
     8881 atagcatgga ccggacgtgg acggacgcgg actctctcgc ctacactcgc tggctggcaa
     8941 cgagccatta cgagaatttc cacgtagttt cgtttcttct tccgaagcgg cttcaccagg
     9001 atttttacaa tgtctatgcg ttctgccgtt gggctgacga cctgggtgac gaaatcggtg
     9061 atcgcgccga gagcctccga ttgctctcct ggtggcgtgg tgagttgtcg gccctctacg
     9121 acgggcaagc agcgcacccc gtattcgtcg cactcagaac gacggtgaag cgccacgatt
     9181 tgccttccga tcctttctcc gacctcatca ccgcgttcga gcttgaccag acaaaagcac
     9241 ggtacgagaa ctgggacgag ctgttttact actgccgcta ttccgcaaat cctgtcggcc
     9301 ggctggtgct ccatctttgc ggctatcacg atgccgggcg ccaggcgttg tccgatgcca
     9361 cctgtaccgc gctgcaactc gccaatcact ggcaggatgt caccgtggat ctcgcaaagg
     9421 atcgtttata tctgccgctc gagttgctgg cgaagcatgc ttgttccgtt gaagatatat
     9481 tcgcgcggca gttcactccg gcgtttgcgg ccgccatgaa agaagcggct gacgttgcca
     9541 ggcgcctgtt cacaacaggg ctgcctctgg ctcgaacagt gaatcgccgg ctcgcgatcg
     9601 atctcgaact cttcagccgc ggtggaatgc gaattctgga taagattgag cggcaaggct
     9661 acaacgttct ggcccagcgc ccctatgttt ccaaggcgga gaaggtgcgg ctgcttttcg
     9721 gggccgtggc tcgggccgtg tttttgagag ctgcatgacg cagacgctcg cacagagcta
     9781 ccggtattgc cggcgtgtgg cgcgcacccg cgccaagaac ttttactact ccttcctgct
     9841 gctttcccgc acgcagcgcg acgcgatgtg cgccgtgtat gcatatatgc gccagtgcga
     9901 cgacttgagc gacgatcccg cccacgggag ccggaagact gcgcgggcct ccctcgaacg
     9961 ctggcgcgtg gatctcgatc gcgcgctcca cggagattac ggcgaaaatc cgctatggcc
    10021 cgcgctcgac gacagtgttc gacgttacgg aatccccccg caatacctgt acgaaatgat
    10081 cgacggcgtt acgtcggacc tggagcctcg ccgcgtcgaa acgttcgagg aactttacca
    10141 atactgttac caggtggcgt cggtggtggg gctcaccgtc atacacattc tagggttcca
    10201 ttcgcccgct gcgttgccgc tggctgagaa gtgcggcgta gcttttcagc ttacaaatat
    10261 cctgcgagac gtccgggagg acgcggaacg ggaccgcatt tatcttccgg ccgaagatct
    10321 ggcttctttc ggcgtcgatc ccgccgcgct ggcgaagggg gataaaaccg gggcttttgc
    10381 ccgcctcatg gaatttgaag cgaaccgtgc gttccgctat taccacgatt ctctcccgct
    10441 ggtgccgatg gtccacaact ccagccgggc ctccctggaa gcgctgattc gaatctacta
    10501 ccgcctgctg gaacgtattc gggattcgga ttatgatgtg ctgcacaggc gtatccgcct
    10561 cgcgccgtgg gagaagatgg cgattcttgc cggaacgctc ctgggccgcc gggaccggtt
    10621 ctggcaaaag catgccgccg gcggcagccg cgcggaggat tgaaagctta tgataactgc
    10681 cgtcagccgc cgggaatttt tgcggacggc atcggccggc gcatttgctc ccgcactccg
    10741 gtcatcgaag ccaaacatcc tgtttttact cacggacgat cagcgatggg atgcactcgg
    10801 ctgcatgggc cggcccgtta tcgagactcc acacattgac cggttggccc gggaaggagt
    10861 gacgttcgaa aacaacttct gcgcgtcggc catttgcatg acgagccggg ccagcatctt
    10921 taccggcctg catgaacgaa cacacggaat cagcagtttc acgcaggatt tcacaccgga
    10981 gcagttgagc gcgacgtatc cgcagattct ccgggaggcg gggtaccgga caggattcat
    11041 aggcaaatat ggggtaggca ataatctgcc ggcggcgcac ttcgattatt tcaaaggctt
    11101 tccgggac
//
LOCUS       DASTMK010000087         4832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_292694, whole genome shotgun sequence.
ACCESSION   DASTMK010000087 DASTMK010000000
VERSION     DASTMK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292694"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFQ79_03600"
     CDS             complement(<1..871)
                     /locus_tag="VFQ79_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0138764.1"
                     /translation="MPRKTKSTLDCWRFFCLAPVVALFVCAAAHAQQRSMVSIEGRTA
                     EVLDDATKINVTVTTPPFLISPTEVTQAEFESVTGYNPSHHKGAGLPVENVTWWEAIR
                     YSNLRSIREGLEPVYDLTNGSWDRSKNGYRLPTDTEWTLALGTLPDAAKMSAFANLGS
                     SDTKDAGALLEFVKTRGTRNAGSLQPTANGLYDMIGNVWEWVYDYANPTSAVYSAAGS
                     SGPGTGVARIVRGGSYASTTSRWARGYRSSIEPDRKSRFTGFRVARNQPAPAAKPARP
                     AWFSEVLTPPAEIG"
     gene            968..2035
                     /locus_tag="VFQ79_03605"
     CDS             968..2035
                     /locus_tag="VFQ79_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEU0138765.1"
                     /translation="MRRRQFLQSATLAPLSSAAASASPGMPLRGLHLAAPRPENMELA
                     QRFFEEVLPKEGVNTLFLEFNYGFRNRRFQEIVDEPALSPEQVRSLARAVRNAGAKLV
                     PQINLLGHQSWAKKTFALLRAHPEFDESPGLYPENEGIYCRSYCPRHPNVHEVVLALV
                     DDLCELCDAHDFHAGLDEVFLLAEDACPRCRGANKADLFAAEVQRIHDHLARSGTTLW
                     MWADRFLDGATTGIGKWEASLDNTHTALPSIPKDVIMCDWHYEAAHPTAIWFALHGFR
                     TASCPWRKASVALRQLDQIQAARRDASGVVAGRLLGMIQTTWVGFTQFAKAYSGDKDA
                     SPQAREAAACFRELFKDLRRS"
     gene            2173..>4832
                     /locus_tag="VFQ79_03610"
     CDS             2173..>4832
                     /locus_tag="VFQ79_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138766.1"
                     /translation="MTLEFQKGKLRIAAACLAVLAMNTTAAYAQVLYSSIVGNVRDES
                     GSAVPGAKVTITNKETNLTRSGDANQEGFYSFPNVPSGTYVVRVSASGFAEFISREVV
                     VAVNTTARVDAPLRVAGVSEQVTVVASEAAVPLQTDRADVRHEISTRQVENLPVPVGR
                     NYQTLFQTLPGFEMEGTVREGRMGGCNPSRSRRFNVNGTSRSGTSTMIDGATSAHIWN
                     VGNSAIVPALESIETVNVTTNSFDAEQGLAGGAVINVQIKSGTNQFHGSAFEYHNNQH
                     LRARQYFLPANQDKGKFISNQYGGTLGGPIKRDKLFFFTSFERRHERENSTAILSVPT
                     ARVRGGDFSDFTVPVYDPLTGDENGVGRTAFAGNLIPANRISPIVGKLLERLPLPNMA
                     EGSETNNFFASVPFAANRWSLDSKANYYVNESFNFFGSISYFDFGITNPTAFGDQLDG
                     ARVGGGNAGRAWGYNNRISFGANYIFTPTLLMDGFFGWGRQNTNVEQPGIGENFGTDL
                     LGIPGTNGPERFQSGWPLFSVSGYAAIGTEEPWTPYYRNDDQISWRANFTKTQGSHEI
                     RWGLDINTERMNHTQPEFQGGSSTGARGRFDFGNGPTALCRIANPTGGCSTLAPTVTQ
                     AQSFSSFLLGLPTQVGKNLLTTFPFTTRSWRYSLYVRDRWQVNQKLTLSFGTRWEYFP
                     MPTRAGRGFERYDPETDKMYIGGVGSVPEDLGVKVSKKLFAPRFGIAYRVTDKTVLRT
                     GYGISIDPYSLSRPLRTNHPILIELVEPSPNNMLPASRLADGIPAIQPVDLGDGIIDV
                     PANVSTQTVPLDFRRGYIQSWNFTVQQDLKWGFVGEVGYVATRQIRQLGYQQLNWAPV
                     GGGNNGRQLFQKFGRTANTREVSPIGGSHY"
BASE COUNT         1011 a         1426 c         1469 g          926 t
ORIGIN      
        1 cgccgatttc cgccggcgga gtcagcacct cgctgaacca ggcgggccgc gcgggcttcg
       61 ccgcgggtgc gggttgattc cgggcgacgc ggaatccggt gaagcggctc ttgcgatcgg
      121 gctcgatcga cgagcggtat ccacgggccc agcgggaagt cgtgctggca taggagcccc
      181 ctcggacgat tcgagcgacg ccggttcccg gccccgaaga gcctgccgcc gagtacacgg
      241 cggacgtggg gtttgcgtag tcgtagaccc attcccagac gttgccgatc atgtcgtaga
      301 gtccattcgc ggtgggttga aggctgccgg cattgcgcgt gccgcgcgtt ttcacgaatt
      361 cgagcagcgc gcccgcgtct ttcgtgtcgc tcgaacccag attcgcgaaa gcggacattt
      421 tggcggcgtc cggcagtgta ccaagggcca gggtccattc cgtatcggtg ggaagacggt
      481 acccgttttt cgagcgatcc catgatccgt ttgtgaggtc gtatacaggc tccagccctt
      541 cgcgaatgct gcgcaggttc gagtagcgaa tggcctccca ccaggtgaca ttttccaccg
      601 gcaggcccgc gcctttgtga tgggaaggat tgtagcccgt cacggactcg aactccgcct
      661 gcgtcacctc ggtgggcgaa atcaggaacg ggggcgtcgt gaccgtgacg ttgattttcg
      721 tggcatcgtc cagaacttcc gccgttctgc cctcgatgga aaccatcgag cgctgttgtg
      781 catgcgcggc agcgcaaacg aacagggcta ccactggcgc caggcagaaa aatctccagc
      841 agtcgagcgt tgactttgtt tttcgtggca tgtataagaa gaccatcatc cgacagttcg
      901 cagccccatg gcggcggacg ccgatgagca ccaatataca ggagtcaacg aaagcgagag
      961 gtgagcaatg cgccgccggc aattcctcca aagcgccacg cttgcgcctt tgtcctcggc
     1021 tgctgccagt gcctcgccgg gtatgccgct gcgtggcctg caccttgccg cgccccgtcc
     1081 cgagaacatg gaactggcgc agcgcttttt cgaagaagta ctgcctaaag agggtgtgaa
     1141 cacactcttt ctggagttca actacggttt ccgcaaccgc cgctttcagg agatcgtcga
     1201 cgagccggcg ttgagtcctg aacaggtacg ttcccttgcc cgcgcagtcc gcaacgccgg
     1261 agctaagctc gtcccgcaga tcaatctgct tgggcatcaa agctgggcca aaaaaacatt
     1321 tgcgctgctg cgagctcatc cggagttcga tgaatcgccg gggctctatc cggagaacga
     1381 aggcatctat tgccgcagtt actgccctcg gcacccgaac gttcacgagg ttgtgctggc
     1441 gctcgtcgat gacctctgcg aactctgtga cgcacatgac ttccacgcag gtttggacga
     1501 agtatttctg ctggcagagg acgcatgccc gcgatgccgt ggtgcaaaca aggcggatct
     1561 gttcgccgct gaggtacagc ggatccacga tcatctggcc aggagtggaa caactctctg
     1621 gatgtgggca gaccggtttc tggatggcgc tacgactggc atcggcaaat gggaggcaag
     1681 cctggataac acgcatacgg ctttgccttc gattccgaag gacgtgatca tgtgcgactg
     1741 gcactacgag gctgcgcatc caacggccat ctggtttgcg ctgcacgggt tccgaaccgc
     1801 gagctgcccc tggagaaagg cctcggttgc attgcggcaa ctcgatcaga tccaggccgc
     1861 gcgccgggac gcaagcggcg tcgtggcggg ccggctcctc gggatgattc agaccacatg
     1921 ggtggggttc acgcagttcg caaaagccta ttccggcgac aaggacgcgt ccccgcaagc
     1981 aagagaggcc gcagcgtgtt tccgcgagct gtttaaagac ctgcgccgca gctaatcgcc
     2041 acccaacgcg ctgccgttac cagctctgaa tattgacttt gttttccgcg gcgtgtataa
     2101 gaagaccatc atctgacagt tctcggcttc atggggatgg acgccgatga gcaccaatgt
     2161 acaggagggg ggatgacact ggaatttcaa aaaggaaaat tgcgcatcgc cgcagcatgt
     2221 cttgccgtgc tggcgatgaa cacgacagcg gcatacgcac aggttctata cagctccatc
     2281 gttggaaatg tgcgcgatga atccggcagc gccgttcccg gcgccaaggt gaccattacc
     2341 aacaaggaaa ccaacctgac gcgaagcggt gacgcaaacc aggaaggctt ctacagcttc
     2401 ccaaacgtgc cgtcggggac ctacgtggtc cgcgtatcgg catccgggtt tgccgagttc
     2461 atcagccgcg aagtggtggt cgcggtgaat accacggcgc gcgtcgatgc tccactgcga
     2521 gtggccggcg tatccgagca ggtgacggtg gtcgcgagtg aagcagcggt accgttgcaa
     2581 acagatcgcg cggacgtacg gcacgaaatt tcgacgaggc aggtggagaa cctgcccgta
     2641 ccggttggac gcaactacca gacgctgttc cagacgctgc ctggattcga aatggaaggc
     2701 accgttcgcg aaggtcgtat gggcggatgt aacccgtctc gctcgcggcg tttcaacgta
     2761 aacggcacca gccggtcggg aacttcgacg atgatcgacg gtgccaccag cgcgcatatc
     2821 tggaacgtgg gcaacagcgc catcgtgccg gctctcgaat ccattgaaac ggtgaatgtc
     2881 acaacgaaca gtttcgatgc ggaacaggga ttggccggcg gcgctgtgat taacgttcag
     2941 atcaaaagcg gcacaaacca gtttcatggc tccgcatttg aataccacaa caatcagcac
     3001 cttcgcgcgc gccagtactt tctgccggcg aaccaggaca agggcaaatt tatctcgaac
     3061 cagtatggcg gcacgctggg cgggccgatc aagcgggaca agctcttttt cttcacgagt
     3121 ttcgaacgcc ggcacgagcg tgaaaactcc accgccatcc tgtcggttcc caccgccagg
     3181 gttcgtggcg gcgacttctc cgacttcacc gtgccggttt acgatccgct caccggcgat
     3241 gagaatggag tggggcgtac cgcgttcgcc ggaaacctga ttccggccaa ccggatttcg
     3301 ccgattgtcg gcaagctgct ggaacgcctg ccgctgccga acatggccga agggtcggag
     3361 acgaataact ttttcgcctc agtgccgttt gcggccaaca ggtggtcgct cgattcgaag
     3421 gcgaactatt acgtcaacga aagcttcaac ttttttggca gcatcagcta tttcgatttc
     3481 ggcattacga accccaccgc gttcggcgac caactggacg gcgcgcgcgt cggcggcggc
     3541 aatgccggca gagcctgggg atacaacaac cgtatcagct tcggagcgaa ctatattttc
     3601 acgccgacat tgctgatgga cggctttttc ggatgggggc gccagaatac gaacgtggag
     3661 cagcctggta tcggagaaaa cttcggaacc gacctgctcg gcatcccggg gaccaacgga
     3721 ccggaacggt tccagagcgg atggccgctg ttttccgtca gtggttatgc ggcgatcgga
     3781 acagaagagc cgtggacacc ctattaccgg aacgacgacc agatctcctg gcgggccaac
     3841 tttaccaaga cgcagggcag ccacgagatt cgctggggcc tggacatcaa caccgaacga
     3901 atgaatcaca cccagcccga attccagggc ggatcttcca ccggcgcacg ggggcggttc
     3961 gacttcggta atgggccgac cgcgctgtgc aggatagcga atccaacagg cggatgcagc
     4021 acgctggccc ccaccgtgac acaggcgcaa agcttctcga gctttctgct cggcttgccc
     4081 acgcaggttg gcaagaacct tctgaccacc tttccattca ccactcgttc ctggaggtat
     4141 agcctctacg ttcgcgaccg gtggcaggtg aaccagaaac tgacgctgtc gttcggaacg
     4201 cgatgggaat actttccgat gccgacgcga gccggacgcg gcttcgaacg ctacgatccg
     4261 gaaacggaca agatgtacat cggcggtgtc ggctcggttc cggaggacct gggtgtaaag
     4321 gtcagcaaga agctgttcgc gccgcgcttc ggcatcgcct accgcgtgac cgataagacc
     4381 gttctgcgca ccggctacgg catcagcatc gatccttact cgctctcgcg accactgcgt
     4441 accaaccacc cgattctgat cgaactggtg gaaccgtctc ccaacaacat gcttccggca
     4501 agccggctgg ctgatggaat tccggcaatt caacccgtag acctcggcga cgggatcatc
     4561 gatgtgcccg cgaatgtttc cacgcaaacc gttccgctcg atttccggcg cggttacatc
     4621 caatcgtgga acttcacggt gcagcaggat ttgaagtggg gcttcgtcgg agaagtggga
     4681 tatgtggcga ccagacaaat ccggcaactc ggctaccagc aattgaattg ggcgccggtg
     4741 ggcggaggaa acaacggacg gcaactgttc cagaagttcg gacggacggc gaatacgcgc
     4801 gaggtatcgc cgatcggcgg aagccactac ga
//
LOCUS       DASTMK010000088         7309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_132323, whole genome shotgun sequence.
ACCESSION   DASTMK010000088 DASTMK010000000
VERSION     DASTMK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132323"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFQ79_03615"
     CDS             complement(<1..291)
                     /locus_tag="VFQ79_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU0138767.1"
                     /translation="MAGARYRTVRDLDWTLLVLTAFICVLGIMQIYSATHDTIWEDAW
                     WKQIVYVVAGAAVMYAVAKIDYQVLLAQVPIFYILSIASLLLTFVIGRQVFGS"
     gene            complement(295..2241)
                     /gene="mrdA"
                     /locus_tag="VFQ79_03620"
     CDS             complement(295..2241)
                     /gene="mrdA"
                     /locus_tag="VFQ79_03620"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU0138768.1"
                     /translation="MKDVPSENNHEELLDLPPETELAPPVLPPRPKIKLASAKIVAFQ
                     YATVAVFVFLLAGFWDLQVRNPEVYSEAAARNRIKGIPILAPRGKILDRDGRVIVDNR
                     SSFSLLLSRENLKPEHLAPIAEGLNLDYDDLLERVKRFQSRPLYEPIIIKEELTPGEL
                     SFVEAHRGFPGFPEMELIRAQRRQYPEGGIAAHVVGYVGEVSDAELNTFEFAKYKPGD
                     VIGKQGIERQYNGRLMGVDGQRQVLVDNRGRERELLAIREAVPGQDLQLSIDLDLQVI
                     AELAMDGRRGAVVALDPRNGEVLAMVSRPSYDPNKFAGRISSKDWKEIVNNPENPLLD
                     RAIQAQLAPGSTFKPVMEIAGLESGVIDDNFKVHCPGGASFYGHYFRCWQSRGHGIVD
                     VHKAMVQSCDVFFYNVGNRLGIDGIAAWAEKLGLGKPTGIDLPHEAAGIVPSTEWKMK
                     TFRQKWYAGETISVAIGQGALTVTPLQLARAIGGIATGGVLVQPHLVKEQTQGEPAKR
                     LDINVDHVNQVIYGMYGVVNEGGGTGARARLVGYDVCGKTGTAQLASNAVLQGTKLGR
                     ELKDNAWFVAFAPRQSPEIVVSVLFEAGEHGSAAGSIARDVLKAYFDKRLRKEADRKQ
                     LARNNLRDSLLFAPGAAFFRQEER"
     gene            complement(2260..2793)
                     /gene="mreD"
                     /locus_tag="VFQ79_03625"
     CDS             complement(2260..2793)
                     /gene="mreD"
                     /locus_tag="VFQ79_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU0138769.1"
                     /translation="MNRYSDELLTELPRRGRPSRYRASVLVVVALAAILFQVYVPLFF
                     EYLSYLELSLLVTVYFSLMKRSPVSGTFIGCAIGLAQDSLSHHPIGMLGIVKSLVGYF
                     SASVSLRFDVDNPLVRMILAFFFFFFHQFFHWVLMRALLGQAINFDFEQTLLFGLFNA
                     IVALPLFHIFDRMRRTG"
     gene            complement(2790..3899)
                     /gene="mreC"
                     /locus_tag="VFQ79_03630"
     CDS             complement(2790..3899)
                     /gene="mreC"
                     /locus_tag="VFQ79_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU0138770.1"
                     /translation="MDSLVNRYRNLTVLVLVIFIQLMLVAYQVKTQQDIRLLRVWAVT
                     AVTPFARVLESVRSRTMGAVNDYLALVNVRAENKRLQEEIGKLKLDNRFLRNELSTAD
                     RVKALAAFQGRTPSRTIPARIIASSPGANSRGFYVNAGSGADVMRGMAVITPDGIVGK
                     VTAAYPTASQVMLLTDPEFAAGVVSQRTGIRGTYKGQGHGTGLIDYIPNEQKVEVGDV
                     FYTSGEDWIFPKGLPVGRVTGVSRGRTFQNIQVAPLGLERGLEEVLIVLEGVHQEVPP
                     PGEQPAAGMHLTRPPQEAAGESLTGENEATARTDADRLRLRYKRLGEAQGHRFGEGGP
                     GTRPPDFNLNPAPALPKERAPAAPSTAPERPPEGQ"
     gene            complement(3933..4958)
                     /locus_tag="VFQ79_03635"
     CDS             complement(3933..4958)
                     /locus_tag="VFQ79_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU0138771.1"
                     /translation="MNVRSLFSLFSSDLAIDLGTANTLVFAKGKGVVVNEPSIVAINK
                     NTGEVEAVGREAKEMLGRTPGNIVAIRPMKDGVIADFKVTERMLNYFIQKAHGRKMLV
                     HPRIVIGVPSEITQVEKRAVIDSAYRAKASEVHLVEQAMVAAIGAGLPITEPSGNMVV
                     DIGGGTTDVAVISLSGIVYSRSVRVAGNEMDDAIMQYLKRKYNLLIGERTAEIIKIQI
                     GSAYPLDRPLTMEVKGRNLIEGVPKTVTIEDKEIREALGECVATIMNAIRVALERTPP
                     ELSADISDRGIVLTGGGALLKNLDKRIREETGLPVSIAEDPLASVVLGTGKMLTDFKL
                     LRRVAIE"
     gene            complement(5095..5964)
                     /locus_tag="VFQ79_03640"
     CDS             complement(5095..5964)
                     /locus_tag="VFQ79_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138772.1"
                     /translation="MKRFPAALVLFFPLLAATHTDWQVELSPALTEWKSEIEATSGGR
                     LIAVRIFTDPVNREIRLPPDIPAIEVLERYLRNTAFLEQFTLAHALTIDYQGVEGRLF
                     FVLLNMALAGEWEGDEDALLAHEFGHVWLNLNGYPSLAFRGAEGCVALHASDIVQHEL
                     LRQDFRRRGIDYDSFWIRNLEKAHLELASRQEQAAEENRCRRLAGLTLWVDVRLGLTS
                     QEWAHRDEFLAALEGRYPEWRELAAELAQRIEKTDLRDRRQYRATLDLTARRIQRHLR
                     GKSYSAETKGLNL"
     gene            6003..7124
                     /locus_tag="VFQ79_03645"
     CDS             6003..7124
                     /locus_tag="VFQ79_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138773.1"
                     /translation="MYSRRSFLIGALAVTGCGRKRGHGFDGYAFVANQEGGAVAAVDL
                     TAFAVVRHIRVNGAPSEVIAHPRRPAVYVLTPASGAVHEIGRDTLTVRRTARLDARAD
                     SMRLSPDGEHLFLLCRESRELVSIGVETLKPEGHVRISGRAFDFDISRDGLYAAMSYP
                     DLRAAEVVALDSRRAKARMVLAGEPGALRFRSDGRYVLIGDRTTRVLSIFETTSGATV
                     THLPLAVSPEHFCFNADGGQMFITGPGMDAVVVVYPHNTPQVAETALAGRTPGFMAAT
                     RPGSASQLLFVTNPNWGHVTILDIRSRRAIATVAVGSSPEFISITPDNQYALVLNRVS
                     GDLAVIRIPTITARRTKAAALFTMIPVGSKPVSADVAYI"
BASE COUNT         1387 a         2300 c         2057 g         1565 t
ORIGIN      
        1 ggacccgaaa acctggcgtc cgatgacaaa agtcagcagg agcgacgcaa tcgagaggat
       61 ataaaaaatc ggaacctggg ccagaagcac ctggtaatcg atcttcgcca cagcgtacat
      121 gacagcggcg ccggccacta cataaacgat ctgtttccac catgcgtctt cccagattgt
      181 gtcatgcgtg gcgctataga tctgcatgat gcccagcaca cagataaatg ccgtcaatac
      241 caggagggtc cagtcaaggt cccgcaccgt gcggtagcgt gcgcctgcca ctgcctaacg
      301 ctcctcctgc cggaagaagg cggcgccggg agcgaacagc aagctgtccc gcagattgtt
      361 ccgtgccaac tgcttccggt ctgcttcttt cctcaatcgc ttgtcgaagt atgccttcag
      421 gacatcgcgc gcgatggacc cggccgccga gccgtgctcg cctgcctcga acagaaccga
      481 taccacgatc tcgggcgatt gccgtggggc gaacgccaca aaccacgcgt tgtctttcaa
      541 ctcgcggcct agtttggtcc cttgcaacac ggcgttggag gcaagctgcg ctgtccccgt
      601 ttttccgcac acatcgtaac ccaccagcct ggccctggcg ccggtgccac cgccttcgtt
      661 caccacgcca tacatgccat agatcacctg gttgacatga tcgacattga tatccaggcg
      721 cttcgcgggc tctccctggg tctgctcctt cacgaggtga ggctgcacca gcacgcctcc
      781 agtcgcgatt ccgccaatcg cacgggccag ttgcagggga gtaaccgtga gcgcgccctg
      841 gccgatagcg acggagatcg tctctccggc ataccatttc tggcggaagg ttttcatctt
      901 ccattcagtg gagggaacga taccggccgc ctcgtgaggc aggtcgatgc cagtcggttt
      961 gcccaggccg agtttctccg cccatgccgc gattccgtcg atccccaggc ggttgccgac
     1021 gttgtaaaag aagacatcgc aggactgcac catcgcctta tggacatcca cgatgccatg
     1081 cccgcgtgat tgccagcacc ggaaatagtg tccgtagaag ctcgcgcctc ccggacagtg
     1141 cactttgaaa ttgtcgtcaa tcaccccgga ttccaaaccc gctatctcca tgaccggttt
     1201 gaaagtggag cccggcgcca gttgggcctg aatggcccgg tccagaagcg ggttctcggg
     1261 gttgttgacg atctccttcc agtccttgga actgattcgt ccggcgaact tgtttggatc
     1321 gtaggagggg cggctcacca tcgccagcac ttcgccgttg cgcgggtcca gcgccacgac
     1381 cgcgccgcgg cggccgtcca tcgccagctc cgctataacc tgaaggtcca gatcgatgct
     1441 cagttgcagg tcctgccccg gaacggcttc ccttatcgcc agcaactccc gttcacgtcc
     1501 ccggttatcc accagcacct gccgctgccc gtccacgccc atcaggcggc cgttatactg
     1561 gcgttcgatt ccctgcttgc cgatgacgtc gccgggtttg tactttgcga actcgaacgt
     1621 gttcagttcc gcgtcgctga cctcgcccac gtatccgacg acgtgtgcgg cgataccacc
     1681 ctcggggtat tgccgccgct gcgcgcggat caattccatc tccggaaaac cgggaaatcc
     1741 ccggtgtgct tccacaaagg agagctcacc gggtgtcaat tcttccttga taatgatggg
     1801 ctcatacaac ggacgggact gaaaacgctt cacgcgttca agcaggtcgt cgtaatccag
     1861 gttcaagcct tcggcaatcg gcgccaggtg ttccggcttc aggttctcgc gcgagagcag
     1921 aagactgaag ctggaacggt tgtcgacgat cactctcccg tcccggtcga ggattttgcc
     1981 cctcggagcg agaatcggta tccccttgat ccggttccgt gccgccgctt cgctgtaaac
     2041 ttccggattg cgcacctgca agtcccagaa tcctgccagc agaaacacga acaccgcgac
     2101 ggtggcgtat tggaatgcga caatcttcgc ggaggcgagc ttgatcttag gccgcggcgg
     2161 cagaacggga ggcgccagtt ccgtctcggg gggcagatcc agcagttctt cgtggttgtt
     2221 ttcgcttggg acgtctttca cgagcggcgg gacttctttt taacccgtcc ttctcattct
     2281 atcgaaaatg tggaacaaag gcagcgccac aatcgcgttg aacaagccaa atagaagcgt
     2341 ttgctcgaaa tcgaagttga tcgcctggcc cagcagcgcg cgcatgagca cccagtggaa
     2401 aaactggtga aagaagaaaa agaaaaacgc caggatcatc cggaccagcg ggttatcgac
     2461 gtcgaagcgg aggctcacgg aagcggagaa atagccgacc aggcttttca cgatgccgag
     2521 catcccgatg ggatggtgcg aaagcgagtc ctgcgcgagg ccgatggcgc atccgatgaa
     2581 cgtgcccgat accggactcc gcttcatcag ggagaagtag accgtgacga gcagcgaaag
     2641 ttccaggtac gacaaatact cgaagaacag cgggacgtaa acctgaaaca ggatggccgc
     2701 taacgccacc accaccagca cgctggcccg gtagcgcgac ggccggcccc ggcgcggcag
     2761 ttccgtcaac agttcgtcgc tgtaacggtt cattgccctt ccggcggtcg ctccggagcc
     2821 gttgatggcg ctgccggcgc gcgttccttt ggaagcgccg gagcgggatt caagttaaag
     2881 tctggcggcc tcgttccggg tcctccttcg ccgaacctgt ggccttgggc ctctcccagg
     2941 cgcttatatc gcagcctcag ccggtccgcg tccgtccgtg cagtggcctc gttttcgcct
     3001 gttagagatt ccccggcagc ctcctgcggc ggccgcgtca ggtgcattcc cgccgccggt
     3061 tgctctcctg gcggtgggac ctcctggtgt actccctcga gtacgatcag aacctcttcc
     3121 agcccacgct cgagcccgag aggcgccacc tggatatttt ggaatgtcct gccccggctt
     3181 acaccggtca ctctcccgac gggcaaccct ttcggaaaga tccagtcttc cccggaggta
     3241 tagaacacat cccccacttc gaccttctgc tcgtttggaa tataatcgat cagtcccgtg
     3301 ccgtgaccct gccccttata cgtccccctg attccggtcc tctgcgaaac gacgccggcg
     3361 gcgaattcgg ggtccgtcag cagcattacc tgcgacgcgg tcgggtaggc tgccgtcact
     3421 tttcctacga tgccatccgg agtgatgacg gccatgcccc gcataacatc cgctcccgag
     3481 ccagcattca cgtaaaatcc tctcgaattg gcgcccgggc tggaggcgat aatccttgcc
     3541 ggaatggtgc gggatggcgt gcggccctga aaagctgcga gcgctttcac gcggtcggcg
     3601 gtggacagct cgttccgaag gaaacggttg tcgagtttga gcttcccgat ctcttcctgt
     3661 agtctcttgt tttccgcccg tacattgacc agcgcaagat agtcgttcac tgctcccatc
     3721 gttctgctgc gcaccgattc cagtacacgt gcaaacggtg tcacggccgt aaccgcccag
     3781 acgcgaagca gccggatatc ctgctgcgtc ttcacctggt aagccaccaa catcagttgg
     3841 atgaagatca ccagcaccag gaccgtcaaa ttgcgatagc ggttaaccag ggaatccatg
     3901 gcacttctgc tacggctctg cggccgttcg ttctactcga ttgccacccg gcgaagaagc
     3961 ttgaaatccg tcagcatttt acctgtgccc agaacaaccg aagccagcgg atcttccgcg
     4021 atcgacaccg gtaggccggt ctcctcgcga atgcgcttat ccaggttttt gagaagggct
     4081 cctccgcctg tcaacacaat gcctcgatcg ctgatatcgg cgctcagttc cggcggtgta
     4141 cgttccaacg ccacgcggat ggcgttcatg atggtggcta cgcactcgcc caacgcctcc
     4201 cggatctcct tatcttcaat agttacggtt ttgggtactc cctcgatcag gtttcgccct
     4261 tttacttcca tcgtgagcgg cctgtccagc ggataggccg aaccgatctg gatcttgatg
     4321 atttccgccg tgcgctcccc aatcagcaga ttgtatttgc gcttcagata ctgcatgatc
     4381 gcgtcgtcca tctcattgcc ggccacgcgc accgatctgg aatacacgat gccggagagc
     4441 gagatgaccg ccacatcggt ggtgccgccg ccgatatcca ccaccatgtt gcccgaaggt
     4501 tcggtgatag gcaggcccgc tccgatggcg gccaccatgg cctgctccac caggtgtact
     4561 tcgctcgcct tcgcccggta tgccgaatcg atgaccgcgc gcttttccac ctgcgtaatt
     4621 tcgcttggca cgccgatgac aatgcgaggg tgcaccagca ttttgcggcc gtgcgccttc
     4681 tggatgaagt aattcaacat gcgctcggtc actttgaagt cggcaataac gccgtccttc
     4741 atcggccgga tcgccacgat gttgccggga gtacgtccca gcatctcttt ggcttccctg
     4801 ccgactgctt ccacttcgcc tgtgttttta ttgatggcga caatcgatgg ttcgttcacc
     4861 accacgcctt tgccttttgc aaacaccagc gtattagcag tgccgaggtc gatagccaaa
     4921 tcggatgaga agaggctgaa cagcgaacgg acgttcatcg atggatttct tttgactcct
     4981 gtcagattcg aaggatctgc tgcctggatg cacgctccgc tccacccgga ggctactatt
     5041 cggaatgtca gggggaagca gtccctttaa agcgtatcat acgaaatctt cgccttacaa
     5101 atttaaacct tttgtttcag cggaatagga cttcccacga agatgccgtt gaatccggcg
     5161 tgccgtgaga tcaagggttg cccggtactg gcgccggtcg cgcaaatccg ttttttcgat
     5221 acgttgcgcg agctctgccg cgagttcacg ccattccggg taacggcctt cgagtgccgc
     5281 caggaattca tcgcggtgcg cccattcctg cgaggtgagc cccagccgga cgtctaccca
     5341 cagagtgagc cccgccagtc tccggcagcg gttctcttct gccgcctgtt cctgccggga
     5401 agcgagttcg agatgcgctt tttccagatt gcggatccag aaagagtcgt agtctattcc
     5461 gcgccgccgg aaatcctgcc gcaacagttc gtgctgcaca atgtcgctgg catgcagagc
     5521 cacgcaccct tccgcgccgc ggaaggcgag cgatggataa ccgttcaggt ttaaccacac
     5581 gtgcccgaac tcatgcgcca gcagggcatc ttcatcgcct tcccattccc cagccagagc
     5641 catgttcaac aacacgaaaa agagccggcc ttccacccct tgatagtcga tggtcaaagc
     5701 atgcgccagc gtgaactgct cgagaaacgc cgtgttgcgg aggtatcgtt cgaggacctc
     5761 gatagcgggg atgtccgggg gtagccgtat ctcccggttg accgggtcgg tgaagatccg
     5821 tacagcaatc agccgtccgc cgctcgtcgc ttctatttca gatttccatt ccgtaagcgc
     5881 cggcgatagc tccacctgcc aatccgtgtg tgttgccgcc aacaagggga agaacaacac
     5941 cagggctgcc ggaaaccgct tcacttggtt caatctagca cgtgctttta caatgatgtc
     6001 tggtgtacag taggagatcg tttctcatcg gcgcgcttgc cgttaccggc tgcggccgga
     6061 aacgcggcca cggcttcgat ggatacgcct ttgtggcgaa ccaggagggc ggtgcggtgg
     6121 cggctgtcga tctgacggcg tttgccgtcg tccggcacat tcgcgtgaac ggcgctccgt
     6181 cggaagttat cgctcatcca cgccgacctg ccgtgtacgt tttgacacct gcctccggcg
     6241 cggtacacga aatcgggcgg gacactctta ccgtacggcg cacggcgcgg cttgacgccc
     6301 gcgccgattc catgcgcctt tcgccggacg gcgagcatct cttcctgctg tgccgcgaat
     6361 ccagggaact ggtctctatc ggcgtggaga cgctcaagcc ggaagggcat gtccggatct
     6421 cgggccgcgc tttcgatttt gacatctccc gcgacggact ttacgccgcg atgtcgtacc
     6481 cggatctgcg cgccgccgaa gtcgtcgctc tcgattcccg gcgtgccaaa gcccgaatgg
     6541 ttctcgctgg tgagccaggg gccttgcggt tccggtccga cgggcgctac gtgttgatcg
     6601 gggaccgcac cacgcgggtg ctttccatct tcgagaccac ttcgggagca accgtaacgc
     6661 accttccgct ggccgtttcc cccgagcatt tctgttttaa tgccgacggc gggcagatgt
     6721 tcatcaccgg cccgggcatg gatgccgtcg tggtggttta tccccacaac acgccgcagg
     6781 tggccgaaac ggcgcttgca ggcaggacgc ccgggttcat ggcggccacg cgtccaggca
     6841 gtgcatcgca gctattattc gttacgaacc cgaattgggg ccacgtcacg attctcgata
     6901 tccggagccg gcgggcgatc gctacggtcg cggtgggctc gtctccggag ttcatcagta
     6961 ttacccccga taaccagtac gctcttgtac tcaaccgcgt gtcgggcgat cttgccgtca
     7021 tccgcattcc cactataacc gccaggcgca cgaaagccgc cgcgctgttc acgatgattc
     7081 ccgtgggttc caaacccgtg agcgcggacg tcgcttacat ttgagggaag cgaaatccgc
     7141 tcccctgaaa gtatcaggca ggtgcagaca ggggttgggc tttcggtttc attgtgactt
     7201 cattgcctag tttctggagc cgctttagga gcgacctttc caggtgcttt ctgttgagcc
     7261 tttcgtagta gtcgtcgccg aggtcccggt aaggcgtacc ggaaacggt
//
LOCUS       DASTMK010000089        10360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_164552, whole genome shotgun sequence.
ACCESSION   DASTMK010000089 DASTMK010000000
VERSION     DASTMK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164552"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VFQ79_03650"
     CDS             complement(<1..1576)
                     /locus_tag="VFQ79_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138774.1"
                     /translation="MNWRRFIERNRRDEELSRELDSYVAHEIDDNLARGMSPIAARQA
                     ALRKFGNLTRVRETIYERNSLHPIDTAWQDLKYGFRQLRLKPGFAMAAILSLALGIGA
                     NTAIFTLVDQILLRLLPVHNPRELVQFEVTGGRFGSNSGDGEHTFSYPIYLAFHDRST
                     VFSGLTGERVERASLTTGDRAEMIRVGMVAGNYFQILGVPLHLGRLFTPDDNKVRNGH
                     PVAVLQYDFWRNRFGANPAIVGSTVHLNGSPFSVIGIGAPNFEGTITGSATQVWVPVM
                     MKPVITPTWDALDNERSTWFYLFGRLKPGVTIDEAQAAMRVLYRQLQEEELKGEHFQK
                     FPEQQERFLRQTFTLVPAARGQSWLRSGFERPLLILQALVGVVLLIACINVANLLLAR
                     AAARQREIGIRGALGAGRRQLIRQFLMENLVLAAAGGIAGLLLSSWMTKGLVRLLPFD
                     PANLSLSTSPDLRILAFTAGITILTAFVFGLVPAFQSAHVSPAITLKEEAGATTGSQG
                     HVRLRKIFVALQVGLSC"
     gene            complement(1581..1934)
                     /locus_tag="VFQ79_03655"
     CDS             complement(1581..1934)
                     /locus_tag="VFQ79_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0138775.1"
                     /translation="MARKTRPDSRGGMLLGTMDMLILKTLLPGAAHGHTIAHVIEKTS
                     EEVLQVEHGSLYPALHRLEDQGLIASFWGTSENNRKAKYYRITPKGKKHLIEERSRWE
                     SLVEAVTRVLGPATE"
     gene            2131..4803
                     /locus_tag="VFQ79_03660"
     CDS             2131..4803
                     /locus_tag="VFQ79_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0138776.1"
                     /translation="MVVLTGPPGSGKTHLIFQQVCQTLAAGRSDFRLLVPSATMAEYI
                     RNRLAREGYVFRPALIQTFSKFIEELTPDLQQASDAAFQAIVDQALESVSPACYTSVA
                     DLDGFRRVLANLIEELSLGGCDPATIHLVPGGEAIAGIYRDVENRLIRRGLTTRAGRL
                     LSCLESNGLPGLSTIYFDGFSSFSNLEIRLLEALAKTAGLTITLPDSTGSASTLERLL
                     SLGFQLRTLTRHRPEAPVVVVAAPDITSEAEEVARRILVHHAEGVRFREMGVVLRSEK
                     PYVPLLETTFERFGIPVRFYFSRPLSSHPVIQFLTGGIDAALGGWDHEALLPVLRFGA
                     AADPSIDRFEFKVRRNLPARGIAPLREMAHGGGVQHLLENLERLDSWRGVALTPPEWE
                     RRIADLTGIVPLETPRDGVSHNLAALWRSRTVALRQFQSAVSDAARTLDPEQAVTLDT
                     FWRVLKGVLRLNSLRVPDHRRDVVHVMDAIEARQWELRNVFVCGLLEKQFPRYHSENP
                     LLSDESRELLNRRGFRFRTTADRQQEEQFLFDVAVSRATAETVLSYPTRDANGEDNLP
                     SFLLSKFQVKVSSQDVRPQSGPPRSRLYSASTSISDSLLQEQLRGKHAVLRPTAVETF
                     LQCPFRFFGRHTLKLDPAPAAPEKRLDALLKGTVVHTVMQRWHSSDGGMEIPSLVNTV
                     IDEICSSEKVPDNFRKELARIEIVAALMKFAEMAEPPEGWKTSAEEPFELRLDGDVVV
                     SGRIDRLDVAPDGRAIVFDYKYSGGKRLKRIMAGHENHKNVQVCLYLLAASRILGHEP
                     AGMFYWSLKGDCPIDGWHVSLADYEKIGAAITSGELMEMLEGSETATLGAASEIRAGR
                     IAPTPDPEICRYCEFRDVCRARSVSKTLAAGDAE"
     gene            4800..8030
                     /locus_tag="VFQ79_03665"
     CDS             4800..8030
                     /locus_tag="VFQ79_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0138777.1"
                     /translation="MTLSTGQRAAVERSGQDVCVVAGPGSGKTRVLVSRFCWLVSQGV
                     SPVRILAITFTEKAATEIKGRIVGQFSQDANLRAQVERAYVSTVHGFCARLLKENAVR
                     AGVDPDFAILDERTSLYELSLAAEEALDEYYRLDPVAFRRLVSGLNAFDITRCLLEVY
                     EARRACPSRDIEPVDTITYAAFLERAEAVLGQSTKGWTPRQAETWRQILSWLEQARTL
                     ESNGDRLDRFRVLGEFDFNLNQVKAGPARDAIREIKNELLPQLKAALISEHYAREHQS
                     LERILQRLDEIYRRRKRMRSALDFADLEEQAIRLLKEDETVRQNTANLFDAVLMDELQ
                     DTNPLQWELMDLVRRRCRFFAVGDINQSIYGFRHAEPAVFALYRDSIRAQGAAVDELR
                     ENHRSRPEILAAVQAVAEVREGVERHELVAARDAAHKAEPSVEVIAAFESQPAEPAEV
                     VEASWIARRILDLHATLTIEENGGRRSLRFSDIAVLVRKSEPMDLIERTLREFGIPAL
                     VSRGRGFFESDEVTDLVHWLRVVSNPRDEISLAAVLRSPLVGVSDETLLRLKACGNLA
                     EGLAACESIVEQPEELERLREARGHLEGARELSGYVSPDRLISPVLDASGYTGSLDAR
                     ARANVEKLLGIRDLYACESMPLAVLTDHLERLRESQSEPEAPIGESTDAVNVMTIHAS
                     KGLEYPVVFVACLHAAVNQSAPTLCHLPGAGLGARWCDPVSGEAVSDTVHKLYSERTR
                     EREKAESDRLLYVAMTRAKEHMVLSFAAGKKMASGWPSAITGAFGIALDGPAKPAEID
                     TFAGFRLKVFRPDRRAESRADDRFTRVEAQPLMLARPALEEQFDSTASVTSLTTYWQC
                     PRRYFLERYLQWPTDRTAPDVFQEDAFDSDTTMEADDFGRAVHAVLAGGPRDNLPADV
                     LEMAGRFETSEFGWRLLRATDAGHECDFVMSFEGLVVRGQIDLWFEEGGELVLIDYKT
                     DRVDPGSDPRRMAAYALQLNLYAAALQKMRGRPVNAAYLYMLHANRAVPVSVDATSLD
                     TVRNIVEALRGSQNRMEFPLKAGEQCFHCPYYKTACPGGTKGSSPV"
     gene            complement(7982..8608)
                     /locus_tag="VFQ79_03670"
     CDS             complement(7982..8608)
                     /locus_tag="VFQ79_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0138778.1"
                     /translation="MTTFLFIRHGLTDAVGTSIVSWLPGVSLNDRGRAQAEDLARRLE
                     HAPIAALYSSPLERTMETAAPAARMLGLEIQVREAFGEVRFGDFSGKRLDALELLPEW
                     KRYNQFRSGIRAPDGELMLEVQARMVAELERLRAEHPGKLIAIFSHADAIRATLAHYS
                     GIPLDLLYRIEISPASVTAIEIADWGPRVLLVNHTGELPFVPPGQAVL"
     gene            complement(8690..9532)
                     /locus_tag="VFQ79_03675"
     CDS             complement(8690..9532)
                     /locus_tag="VFQ79_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138779.1"
                     /translation="MTGSTTPQFQELEIPVAMIEHQVERILASDVFIRSERMSRFLRF
                     TVDQVINGSGESLKEYLIGLAVYDKPETLGPRFNPIVRVEAGRLRSKLREYYEHEGRH
                     DPVLISYPKRSYAPHFNFRTLPGVLPIRDSYREAQALQSLDGKLQRPVASIAVLPLID
                     LSPGRDQSELCEGLCRRIIDCIAQSGGIRVLRTGGEAGDVRMIGRRLGVEAVLEGSIR
                     SSHAQLRVCLQLNSTQDGFHLWSETHDGDKRQRRALERQVSAAVLARIKPEHAGMRPL
                     SRAS"
     gene            9836..10096
                     /locus_tag="VFQ79_03680"
     CDS             9836..10096
                     /locus_tag="VFQ79_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU0138780.1"
                     /translation="MFSIIGWIVFGLVVGVIAKLLMPGKDPGGFIVTVLLGIAGALLG
                     GYMGRALGWYGPGEGAGFLMALFGAIILLLLYRLAFRRGTVG"
BASE COUNT         2066 a         2978 c         3231 g         2085 t
ORIGIN      
        1 tgcacgagag cccgacttgc agggccacga aaatcttgcg cagccgcaca tgcccctggc
       61 tgcccgtggt ggccccagcc tcttctttta aagttatggc gggcgaaacg tgcgcgctct
      121 gaaaagccgg aaccagcccg aaaacaaagg cggtgagaat ggtgatacca gcggtaaagg
      181 cgaggatccg aaggtccggc gaagtggaga gggaaaggtt ggcgggatca aacggcagca
      241 ggcgcaccag gcccttggtc atccacgaac tgagcaacag ccctgctatg ccgcccgcgg
      301 cggcgagaac caggttctcc atcaggaatt gccggatcag ttgcctcctg ccggcgccca
      361 gcgcgccgcg aattccaatc tccctctggc gcgcggcggc gcgtgcgagc aacaggttcg
      421 ccacattgat gcacgcgatg agcagaacga cgccgaccag tgcttgcaga ataaggagcg
      481 gccgttcaaa accgctccga agccaggact ggcctcgtgc ggccggaacc aacgtgaagg
      541 tctgccgtag gaaacgttcc tgctgctcgg gaaatttctg aaagtgctca cccttcagct
      601 cttcctcttg aagctgacgg tagagtacgc gcattgcagc ctgtgcttcg tcgattgtga
      661 caccgggctt caggcgtccg aaaaggtaaa accacgtaga gcgctcgtta tcgagcgcat
      721 cccaggtggg cgtgatgaca ggcttcatca tcactggaac ccacacctgc gtcgcggagc
      781 cggtaatggt gccttcaaaa ttcggagcgc cgatgccaat cacggagaag ggtgagccgt
      841 tcagatgaac ggtggaacct acgatggcag ggttggcccc gaaacggttc cgccaaaaat
      901 cgtattggag aacggccaca ggatggccgt tgcgcacctt attgtcatcc ggagtgaaca
      961 atcgccccag gtgcagtgga acacccagaa tctggaaata gttccctgca accattccca
     1021 ccctgatcat ctcggcgcga tctccagtgg ttaaactggc acgttccacc cgctcgccgg
     1081 tgaggccgga aaagacggtg ctccggtcgt ggaaagccag gtaaatggga taggagaacg
     1141 tgtgttcacc gtcaccggag ttggagccga agcgccctcc cgtgacttca aactgaacca
     1201 actcgcgcgg gttatgcacc ggcaggaggc gtagcagaat ctgatcgacc agcgtaaaaa
     1261 tcgccgtatt ggccccgatc cctagtgcca gcgagagaat cgcggccatg gcgaatccag
     1321 gtttcaagcg gagttgccgg aagccgtact tcagatcctg ccaggcggtg tcgatcggat
     1381 gcaggctgtt ccgttcatag atggtctccc ggacacgagt gagattgccg aacttgcgca
     1441 atgccgcctg gcgagccgcg atggggctca tcccgcgcgc caggttatcg tcgatctcgt
     1501 gagcgacata agagtcgagc tcgcgagata gttcttcgtc gcgcctgttg cgctctatga
     1561 atcgccgcca gttcatggca tcactccgtc gcgggaccca atacgcgcgt cacggcttcc
     1621 acgagcgatt cccagcggct gcgttcctcg atcaggtgtt tcttgccttt cggcgttatg
     1681 cggtagtact tcgctttgcg attgttctcg gatgtgcccc aaaaggaagc gatcagtcct
     1741 tgatcttcga gacgatggag cgcggggtag agcgatccgt gctcgacttg caggacctcc
     1801 tccgatgtct tctcgatgac gtgagcgatg gtgtgtccgt gagcggcgcc aggcaggagc
     1861 gtcttgagaa tcagcatgtc cattgttcca agcagcatgc cgccgcggct gtccggtcgc
     1921 gtctttctgg ccatagtcca gattgtacca gaagatattc tagtggaaac gcaaaaagac
     1981 cgcttgctgg cgcgcgcggc tcagaattag ctcgttacag agccacgacc gcaagggagt
     2041 ggaggcttcg ggccgttttt caccaagctc ctaaagagga tctacctgag gttcgggagt
     2101 taacggggcg gggcgttata ctgggcagga atggttgttc tgacgggtcc gcccggttcg
     2161 ggcaagaccc acctgatatt tcagcaggtt tgtcaaactc ttgctgccgg ccggtctgat
     2221 tttcgcctcc tcgttcccag cgctacgatg gcggagtata tccgcaaccg gttggcgcgg
     2281 gaaggttacg tattccgtcc cgccctcatt cagacgtttt ccaagttcat cgaggagttg
     2341 acccccgacc ttcagcaggc ctccgacgcc gcatttcagg ccatcgtgga tcaggcactc
     2401 gagagcgtgt ctcccgcctg ctacacgtcc gtcgcggatc tcgatggatt tcgccgcgtt
     2461 ctcgccaacc tgatcgagga gttgtcattg gggggatgcg atccggctac gattcacctg
     2521 gtgccgggcg gcgaagcaat cgccggtatc taccgggacg tggagaaccg ccttatcagg
     2581 cgcgggctga cgacgcgagc gggccgcttg ctttcctgcc ttgaaagcaa cggactgccc
     2641 gggttgagca ccatctattt cgatggtttt tcatcgttct ccaatctcga aatcagactg
     2701 ctcgaagcgc tcgcgaagac ggccggtctt accatcacac tgccggattc aaccggatcg
     2761 gcaagtacgc tggaacgcct gctttccttg ggctttcagc ttcgcacgct cacaaggcac
     2821 aggccggaag caccggtcgt agtggttgcc gctcccgata ttacgagcga agccgaagaa
     2881 gtggcccgac gcatcctcgt gcaccatgcc gaaggagtcc gcttccgtga aatgggcgtt
     2941 gtgctgcgat cggaaaagcc ctatgtcccg ctgcttgaaa ctacgttcga gcggttcggt
     3001 attccggtac gtttctattt ttcgcggccg ttgagcagcc atcccgtgat tcagttcctg
     3061 acgggcggaa tcgatgcggc gcttggtggg tgggaccatg aggcgctgtt gcccgtcttg
     3121 cgttttggtg cggcggcgga tccgtccatc gaccgtttcg agttcaaggt tcgaagaaat
     3181 ttgccggcgc gcgggattgc accgctgcga gagatggcac acggcggcgg tgttcaacac
     3241 ctgctcgaaa atctcgagcg gttggattcc tggcgaggag tggcgctaac gccgcctgag
     3301 tgggagcgga ggattgcgga tctcaccggc atcgtgccgc tggagacgcc aagagatggc
     3361 gtatcgcaca acctcgcggc cttgtggcga agccggaccg tcgcccttcg ccagtttcaa
     3421 tccgccgtga gcgatgcggc gcgcacgttg gaccccgaac aggctgtcac gctcgatacg
     3481 ttctggcgcg tgctcaaagg cgtgctgaga ttgaacagcc tgcgcgtgcc cgaccacaga
     3541 cgcgatgtgg tgcatgtgat ggacgcgatc gaagcgcggc agtgggagct gcggaatgtc
     3601 ttcgtatgcg ggctgctcga gaagcagttt ccgcgctacc actcggaaaa ccccttgctt
     3661 tccgacgagt cccgggaact gctgaaccgg cgtggattcc gttttcgcac gacggcggac
     3721 aggcaacagg aggagcagtt tctgtttgac gttgccgtaa gtcgggccac cgctgagacg
     3781 gtgttgagct atcccacgcg cgatgccaat ggagaagata acctgccgtc gtttctgctc
     3841 tcgaaattcc aggtgaaggt ttcgagccag gacgtgcggc cacaatccgg accgccacgc
     3901 tcccgcctct attcggcgtc tacgtcgatt tcggactccc tcctccagga gcaacttcgc
     3961 ggcaagcatg ccgtgctccg gccgacagcg gttgaaacgt tcttgcagtg ccccttccgc
     4021 tttttcgggc ggcacacact gaaactcgac ccggcaccgg ctgcgccgga aaagcgtctg
     4081 gatgcgcttc tcaagggcac ggtggtgcac accgtgatgc agaggtggca ttccagcgat
     4141 ggcggtatgg agattccgtc tctcgtaaat accgtgatcg acgagatctg ttccagcgaa
     4201 aaggtccccg ataatttccg aaaagaactg gcgcggatcg agattgtggc cgccctgatg
     4261 aaattcgctg aaatggcgga gccgccggag ggatggaaga ccagcgcgga ggagccgttc
     4321 gagctgcggt tggatggcga tgtggttgta tccggccgca tcgaccgttt ggacgtcgcc
     4381 ccggacggcc gcgccatcgt gtttgattac aagtacagcg gcggcaaacg tctgaagcgg
     4441 atcatggcgg gccatgaaaa ccacaagaac gtgcaggtgt gcctctatct gctcgccgcc
     4501 tcccgcatcc tgggacatga acccgcggga atgttttatt ggagcctgaa gggagattgt
     4561 ccgatcgacg gctggcatgt ttcgctggcg gactatgaaa aaatcggcgc ggcgattaca
     4621 tccggcgaac tgatggagat gttggagggg agtgagacgg caacgctcgg agccgcctcc
     4681 gaaatacgcg cgggccgtat tgcgcctacc ccggatcccg agatctgccg ttactgtgaa
     4741 ttccgcgatg tgtgccgcgc gcgttccgtt tcgaaaacgc tcgccgccgg agacgctgaa
     4801 tgacactcag caccggacag cgggcggcgg ttgagcgcag cgggcaggac gtctgcgtcg
     4861 tcgcgggacc cggttcggga aaaaccaggg tcctggtctc gcgattctgc tggctggtga
     4921 gccagggcgt gtcaccggtt cgcatcctgg ccattacgtt caccgagaaa gccgctaccg
     4981 agatcaaggg aagaattgtc ggccagttct cgcaggatgc caacctgcgc gctcaagtgg
     5041 agcgagctta tgtctccacc gtgcacggct tctgcgcgcg gctgctgaag gagaacgccg
     5101 ttcgagcggg cgtcgacccg gacttcgcca ttctggacga acgcacgtcc ctgtatgagc
     5161 tgtcgctcgc ggccgaagaa gccctggacg agtactaccg cctggatccc gtggcgttcc
     5221 ggcgtctggt ttccggcctc aatgcgttcg acataacacg ctgcctgctg gaggtctatg
     5281 aagcccgccg cgcttgtccc tcgcgggaca tcgagccagt ggacacgatt acttatgcgg
     5341 ctttcctgga gcgggcggaa gcggttctgg gtcaaagcac aaaaggctgg acacccagac
     5401 aagccgaaac ctggcgtcag atcctctcct ggttggagca ggcgcggacg ctcgaaagca
     5461 acggcgatcg attggaccgg ttccgggtcc tgggggagtt cgatttcaac ctgaaccagg
     5521 tgaaagcggg gcctgcgcgc gacgcgatac gcgaaatcaa aaatgaactg ctgccacagc
     5581 tcaaggcggc gctgatctcc gagcattacg cccgcgagca tcaatcgctg gagcgaattc
     5641 ttcaacgcct ggatgagatc taccggcggc ggaaacggat gcgaagtgcg cttgatttcg
     5701 ccgatctcga agagcaggcg atccggcttc tgaaggaaga cgagacggtc cggcaaaata
     5761 ccgcgaattt gtttgatgcc gtcctgatgg atgaattgca ggacaccaac ccgttgcaat
     5821 gggagctgat ggacctggtg cggcggcgtt gccgtttttt cgcggtcggc gacatcaatc
     5881 aatccatcta cggattccgc cacgccgagc ccgccgtgtt cgcgctatac cgcgacagta
     5941 ttcgcgcgca aggggcagcg gtggacgagc tgcgcgagaa ccatcgctcg cgcccggaga
     6001 ttctggcggc cgtgcaggcg gtcgccgagg tcagggaagg cgtagagcgt cacgaactgg
     6061 ttgccgcacg cgatgccgca cacaaggctg agccctccgt ggaagtgatc gccgccttcg
     6121 aaagccagcc agccgaacca gcggaggtgg tggaagcaag ctggattgcg cgccgcattc
     6181 tcgatctcca tgcgacgctc accatcgaag agaatggcgg tcgaaggtcg ctccggttta
     6241 gcgatattgc cgtgctggtg cgcaaatccg agccgatgga tctgattgaa cgaacgctgc
     6301 gcgagtttgg aataccggca ctggtaagcc gcggcagggg atttttcgag tcggacgaag
     6361 tgaccgacct cgtgcactgg cttcgcgtcg tttcaaatcc acgcgatgag atcagcctgg
     6421 cggccgtcct gaggtcgccg ctggttgggg tttcggacga gacgcttctg cggctcaaag
     6481 cgtgcggcaa cctggcggaa ggactcgcgg cctgcgagtc gattgtagaa cagcccgagg
     6541 agttggagcg ccttcgcgaa gcgcgtggcc atctcgaagg cgcacgcgaa ctgtccggct
     6601 atgtttcacc ggacaggctg atctcacccg tcctggacgc ctccgggtat accgggagcc
     6661 tcgatgcacg cgcgcgcgca aacgtggaga agctgcttgg aatccgcgat ctttatgctt
     6721 gcgagtccat gccgttggcc gttcttacgg accacctgga gcggttgcgt gaatcgcaat
     6781 ccgagccgga ggcccccatt ggcgaaagca ctgatgctgt aaacgtaatg acgattcatg
     6841 cgtcgaaggg tttggagtat ccggttgtct tcgtcgcgtg cctccatgcg gcggtgaacc
     6901 agtccgcgcc tacgctgtgt cacctgccgg gagccggact tggagcgcga tggtgcgatc
     6961 ctgtttcggg cgaagccgta tccgataccg ttcataaact ctactccgag cgaacccgag
     7021 agcgcgagaa ggcagagagc gatcgcctcc tgtacgttgc catgacgaga gccaaagaac
     7081 acatggtgct ttcgttcgcc gcgggaaaga agatggcctc gggctggcct tccgcgatta
     7141 cgggcgcttt cggaatcgcg ctggatggac cggcgaaacc ggctgagatc gatacatttg
     7201 ccgggttccg gctgaaggtc ttccggccgg atcgacgtgc cgaatccagg gcggatgacc
     7261 ggttcacgcg cgtggaagca cagcctctca tgctggcgcg ccccgcactc gaagaacagt
     7321 ttgattccac tgcctccgtc acttcgctta ccacctattg gcaatgccct cgccggtact
     7381 tcctcgagag atatttgcag tggcctacag atcgcacggc tccggatgta tttcaggaag
     7441 acgccttcga ttccgatacc accatggaag ccgatgattt cggccgggcg gtacacgcgg
     7501 ttttagcggg cggcccgcgt gacaacttgc ctgcggacgt gttagagatg gccgggcgtt
     7561 ttgaaaccag cgaattcggg tggcgtcttc ttcgagccac cgacgcggga catgaatgcg
     7621 atttcgtgat gtcgtttgaa ggtctggttg tgcgcgggca aatcgatctg tggtttgaag
     7681 agggcgggga actggtgctg atcgattaca agaccgatcg ggtcgatccc ggctcggacc
     7741 cgcgcaggat ggcggcatat gctctgcaat tgaacctcta tgcggccgca ctccaaaaga
     7801 tgcgcggaag gccggtgaat gccgcttatc tgtacatgct gcatgccaat cgtgctgtgc
     7861 cggtttccgt cgatgcgacc tctttggaca cggtgaggaa tattgtggaa gccttgcgag
     7921 gatcgcagaa ccggatggag tttcctctta aagccggcga gcagtgcttt cactgccctt
     7981 actacaagac ggcttgtccg ggcggcacga aaggcagctc gcccgtatga tttaccagca
     8041 ggacgcgcgg tccccagtcc gcaatctcga ttgcggttac ggacgccggg ctgatctcga
     8101 tgcggtaaag cagatccagc ggaatccccg agtaatgcgc cagggtagct ctgatggcat
     8161 cggcatggct gaaaatcgcg atgagtttgc ccgggtgctc cgctcgcagc cgctccaact
     8221 cggcaaccat gcgggcttgt acttcgagca tcagttcgcc atccggtgcg cggatgccgc
     8281 tgcgaaactg gttatagcgt ttccattctg gcaatagctc cagcgcgtcg aggcgctttc
     8341 cggagaaatc tccgaaacgc acctcgccga atgcttcccg tacctgtatt tcgagtccca
     8401 gcatcctggc ggcgggcgcc gcggtctcca tcgttctctc gagagggctg ctgtagagag
     8461 ccgcgatggg cgcgtgttcc aaacgccggg ccagatcctc cgcctgcgcg cgcccccgat
     8521 cgttcaaaga aaccccgggc aaccagctaa cgatggaggt gccgacggca tccgtcaacc
     8581 catggcggat gaaaagaaat gtagtcacca aacggctacc gtagcacacc ggggaagtgg
     8641 aacagccgaa ggaacttagc gtctcggcgg gcgtagcatg ccgccgccgt caactggcgc
     8701 gggaaagcgg gcgcattccg gcatgttccg gcttgatacg tgcgagcacg gccgcgctca
     8761 cctgccgctc cagcgcccgc ctctggcgct tgtcgccatc gtgggtctcc gaccacagat
     8821 gaaagccatc ctgcgtgctg ttcaattgca ggcagacccg taactgcgca tgagaacttc
     8881 ggatactgcc ttcgagaacc gcttccacac cgagacggcg gccaatcatc cggacatccc
     8941 ccgcttcgcc accggttctc aacacccgga taccgccgga ctgcgcgatg cagtcgatga
     9001 ttctccggca aaggccctca cacaattcgg actgatcccg gccggggctc agatcgatga
     9061 gaggcagaac agcgatggag gccaccggac gctgcaactt gccatcgagg gactgcaaag
     9121 cttgtgcctc ccggtagctg tcgcgaatcg ggagaacgcc cggcagcgtg cggaaattga
     9181 agtggggagc ataacttcgc ttcggataac taatcaggac tggatcgtga cgcccctcgt
     9241 gctcataata ttcgcgcagt ttggaacgta agcgcccggc ttcgacgcga acaatcgggt
     9301 tgaaacgggg gccgagcgtc tccggcttgt cgtaaaccgc cagcccgatc aggtattctt
     9361 tcaaactctc tccagatccg ttgataacct gatccactgt gaaccggaga aaacggctca
     9421 tgcgttcaga ccggatgaat acgtcgctgg caagtattcg ttccacctgg tgctcgatca
     9481 tcgctacggg gatctctaac tcctggaact gaggcgtggt tgaaccggtc atctgcagcc
     9541 cttccgaacc agcgcgtcaa cagcaacact cgtaccaaag tactagtcgg gagggattct
     9601 cacggttgag acagcttagc gggctaaacc gaacatggca ggcggaggac gggcggttac
     9661 gaatggaaat tttttccggt cccggaaatt gtttcctctt tttatttcct gcgaatcccg
     9721 tagtttgccg agacggcggc aacaggcgcg tggtgttacg ttgttccgct attggcgcgg
     9781 aacgtgcatt gggcggatgc gcggatgaag acaccgcagt tctgagggag gaagcatgtt
     9841 ttcaatcatc ggttggattg tgttcgggct ggtcgtaggt gtcatcgcga agctcctgat
     9901 gcctggaaag gatccaggcg ggttcatcgt cacggttctg ttgggaatag caggtgcatt
     9961 gcttggcggc tatatgggac gcgcgctggg ctggtatggt cccggcgagg gcgccggatt
    10021 tctgatggca ctgtttgggg ctattatcct gctgctgctc taccggctgg cattccggcg
    10081 cggtacggtc ggataaatct cacgggtact accgcacgac accggttgcg gccgggaacg
    10141 agaattcatt ctgaggctgg gggaaggttc atggctaatc aagaaacaat gacaacacaa
    10201 gggcaacagc caatgacaga ggaagagtac gggaatgcct accagagagg attcagtttg
    10261 acggtccgat ttctggtctc caaggggcta tcgtacgatt cggcgcgcga gactgcgcag
    10321 gccgcatggg ccaaaggttg ggagcggctc ggccagctcc
//
LOCUS       DASTMK010000090         4593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_132601, whole genome shotgun sequence.
ACCESSION   DASTMK010000090 DASTMK010000000
VERSION     DASTMK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132601"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1956
                     /locus_tag="VFQ79_03685"
     CDS             <1..1956
                     /locus_tag="VFQ79_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138781.1"
                     /translation="ELIDPRPESIKEVTTPDDHAGVRFLTGGPEPGNISALAIDPADS
                     KILYAAVSSGAGAVLRSSDDGAESWRDAGVIPVAATRLYIDPKSPKSSRTIYAIGSRS
                     VAVRENGAWGENPGPLTTGSFSGTSAGFPSDGGKPVIYAVSGANLFVSEDGGVSWRSS
                     SVAGLDAARYETIATSLNHPDVAYVSIGRATADGVSVFGVAKTTNRGARWDFIRRETS
                     DTPGAGMHDAWLTRRFGPGWGESPLGLGVAPNDPDIVYSTDYGRTMRSTDGGSNWMAV
                     YSKEVEGGGYTTRGLDVTTNYGIHFDPFDAKRIFISYTDIGLFHSEDGGRSWFSSTTN
                     GVPRRWVNTTYWLEFDPEVKGRMWAVMSGNHDLPRPKMWRTQNPSNYVGGVARSDDGG
                     KTWTRTTNGMGETAATHILLDPTSPKESRVLYVAGFGRGVFKSTDGGETWTLKNNGLP
                     AHQPFAWRLTRDQNGVLYLVIARRSENGSFGNAEDGLLYRSDDGAEHWTKIELPAGMN
                     GPNGLAVDPANPRRLYLAVWGRRATDRAVDGGIFLSTDAGASWRNVLSKDQHVYDVTI
                     DPRNANILYACGFESSAWKSTDRGETWSRIPGYDFKWGHRVFPDPRDASKIYVTTYGG
                     SVWHGPAGGADSPRVTPPSGRSPKTRR"
     gene            complement(1916..2776)
                     /locus_tag="VFQ79_03690"
     CDS             complement(1916..2776)
                     /locus_tag="VFQ79_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0138782.1"
                     /translation="MKNRLWLLFALVTTVFWGIWGALIEVPEKAGFPATLGYSVWALT
                     MVPCALIALKIAGWKLQHDRRTVALGMTVGLLGAGGQIILFQALRLGPAYLIFPIVSL
                     YPVLTVILSVWVLKERARRKSWAGIILAFPAIAMLSYQPPGSSITTSQWWIVLASCVF
                     VAWGVQAFVMKVSTNDIAPESIFFYMMLSGLLVIPAALVMTDFSQPINWGFRGPWAAA
                     LIQVLNSIGALCLVYAVRYGKAIIVVPMTALAPVLTVIISLALYQVVPNAVVTAGLIC
                     ASLAIYLMAE"
     gene            complement(2871..>4593)
                     /locus_tag="VFQ79_03695"
     CDS             complement(2871..>4593)
                     /locus_tag="VFQ79_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0138783.1"
                     /translation="YFFHGWSERFNKPPRSGKGYDSGDDYGGDNIAAFVSRNDLIVVR
                     WDGYNPRTPGEDYPRPYNISPVETHRQFPLYFPELVRYIDSNYRTIPDRDHRATAGLS
                     MGGFMSYWVGGKYPHLVGSVSNFMGSSEFYVGPKEFPSEYRHTEMYRNYEGLRTRIVV
                     GSKDFIRWYHRRMNAVWDFVRPHHEHEEFDSEHGTPGMAKTLQFHMNAFRNPLPRPPV
                     WHHIEVYPEFEVWGYAVQTDRQQPGFTLIENVTAKGFRSSVREWVPSGRLLSSVNVRI
                     TTDAIYRPQASYEITDVNVSTGEARRSTMKADSSGRLQFTLDGGLHEIGINETKAPIL
                     TISGWRVVEAPWATAGKQVRLKLTVLNKGAADARNARVQVSSPNSGVSIPQADFALPL
                     VLAGKSVELRQDLLIAIQDPDREIVQLKFSAAGYETPVEIPVFREAPELSGIVILDGA
                     KQRVWQRAVHSTEKTLGTGNGDGQAHPGESVVLGVPDADALRLVEVFTGDACADVSTR
                     ISDPWGAYDNVGATAKYSVVQLSSSCGAGSEIPLFVRYQLPDKPEHALKEGTVRIRLQ
                     APAVTKK"
BASE COUNT          957 a         1423 c         1369 g          844 t
ORIGIN      
        1 gagctgattg atccacgtcc ggagtccatc aaagaagtga ccacgccgga cgatcacgcg
       61 ggcgtccgct ttcttacggg aggtcctgaa cccggcaaca tctccgctct ggcgatcgac
      121 cccgccgatt ccaaaatcct ctacgcggct gtttcttcag gcgccggcgc ggtgctgcgc
      181 tcgtcggacg acggtgcgga atcctggcgc gatgccggtg taattcccgt cgcggccacg
      241 cgtctgtata tcgacccgaa atctccgaag agcagccgga ccatctatgc aatcggtagt
      301 cgctcggttg ccgtacgcga gaacggcgca tggggggaaa atccggggcc gctcacaacg
      361 ggctcattca gtggaacctc ggccggattt ccctccgatg gcggcaaacc ggttatctat
      421 gccgtatccg gcgcgaatct tttcgtgtcg gaagacggcg gcgtgagttg gcgctcctcc
      481 agtgtcgccg gcctggacgc ggcccgatac gagaccatcg cgaccagcct gaaccatccc
      541 gacgtggctt acgtctccat cggtcgcgcc acagccgatg gagtaagtgt ttttggcgtc
      601 gcaaaaacca cgaatcgcgg cgcgcgttgg gatttcattc gccgggagac atccgataca
      661 ccgggcgcgg gcatgcacga tgcctggctc acccgccggt ttggcccggg gtggggggag
      721 agcccgctgg gcttgggcgt cgcaccgaac gacccggaca ttgtctacag caccgactac
      781 ggccgcacca tgcgcagcac cgacggcggc agcaactgga tggccgtcta ttcgaaggaa
      841 gtcgagggcg gcggctatac cactcgcggt ctcgatgtga cgactaacta cggcattcat
      901 ttcgacccgt tcgatgcgaa gcggattttc atctcctaca ccgacatcgg actgtttcac
      961 agcgaagacg gcggccggag ctggttcagt tccaccacga acggcgtgcc gcgccggtgg
     1021 gtgaacacaa cctactggct cgaattcgat ccggaagtga aaggacgcat gtgggccgtg
     1081 atgagtggca accacgatct gccccggcca aaaatgtggc gcacgcaaaa tcccagtaac
     1141 tatgtcggcg gcgtcgcccg cagcgacgac ggtggaaaga cctggaccag aacgacaaat
     1201 ggcatgggag agacggctgc aacccacatc ctgctcgacc ccaccagccc gaaggagtcc
     1261 cgcgttctct acgtcgccgg attcggacgc ggtgtattca aatccaccga cggcggcgag
     1321 acctggacac tgaagaataa cggcctgccc gcacaccagc ctttcgcctg gcgtctgacc
     1381 agggatcaga acggcgtgct ttatctcgtt atcgcgcgcc gcagcgaaaa cggcagcttc
     1441 ggcaatgcgg aagacggctt gctatatcgc tccgacgacg gcgcggagca ctggacgaag
     1501 atcgaactgc cggcgggcat gaatggcccg aacggcctcg cggtggatcc tgcaaatccc
     1561 cggcggctgt acctggccgt gtggggacgc agagcaacgg accgcgccgt ggacggcggg
     1621 atctttctct cgacggacgc cggcgcttcc tggcgcaacg tcctctcgaa agatcagcac
     1681 gtttacgacg tcaccatcga tccgcggaac gcgaacatcc tctatgcctg cggcttcgag
     1741 tcgtcagcct ggaaatccac cgaccgcggc gaaacctgga gccggattcc cggctacgac
     1801 ttcaaatggg gccaccgggt attccccgat ccgcgcgatg cctcgaagat ctatgtaacg
     1861 acctatggcg gaagcgtatg gcacggtccc gccggcggcg cagatagccc tcgggttact
     1921 ccgccatcag gtagatcgcc aaagacgcgc agataaggcc tgcggtcaca accgcgttcg
     1981 gcaccacctg gtacagcgcg agggaaatga tgacggtcag caccggcgcc agggcggtca
     2041 tcggcaccac gatgatcgcc ttgccgtatc gtacggcgta gaccaggcac aatgctccga
     2101 tggagttcag cacctgaatc agggccgcgg cccatgggcc gcggaagccc caattgatcg
     2161 gctgcgagaa atccgtcatg acgagcgccg ccggtatcac cagaagaccg ctcagcatca
     2221 tgtagaagaa tatgctctcc ggagcgatat cgttcgtcga aaccttcatc acgaaggcct
     2281 gcacacccca ggcgacgaac acgcacgagg ccaatacaat ccaccactgg ctggttgtaa
     2341 tcgagcttcc cggcggctga tacgaaagca tcgcgatggc gggaaaggcg agaataatgc
     2401 cggcccagct tttccttcgg gctctctctt tgagaaccca cacggataaa atgacggtca
     2461 gtaccggata cagcgacacg atgggaaata tcagataagc cggaccaagc cgcagcgcct
     2521 ggaatagtat gatttgtccc cctgcgccca gcagacccac ggtcataccg agagcgaccg
     2581 tccgccggtc gtgctgcaat ttccagccgg caatctttag tgcgatcagc gcgcagggca
     2641 ccatggtcag cgcccaaacg gagtaaccga gtgttgccgg aaatccggct ttctcgggaa
     2701 cttcgatcaa ggcgccccag atgccccaga agacagtggt tacaagtgcg aaaaggagcc
     2761 agagccggtt cttcagaaaa aaagtcctcc ttcaaaaaaa aatagcgccc ggcccgtaaa
     2821 agggcccggg cgctaagtac aagatactac cttcgctgtt cagatgccga tcatttcttt
     2881 gtgacggcag gcgcctgtag acggatgcgc acggtgcctt ccttcagcgc atgctccggc
     2941 ttgtccggta actggtaacg cacgaacagc gggatctcgc ttccggcgcc gcaggacgac
     3001 gacagttgca cgacggaata cttggccgtc gcgccgacgt tatcgtaggc gccccaggga
     3061 tcggagatac gtgtcgacac atccgcgcag gcgtcgcccg tgaacacttc cacaagccgc
     3121 aaagcatccg cgtcgggcac gccaagcacg acgctttcac ccggatgcgc ctggccgtcg
     3181 ccgttgcctg ttcccagcgt cttttctgtc gagtgaacag cacgctgcca aacacgctgt
     3241 ttcgcgccgt ccaaaatcac aatacccgaa agctcgggcg cttcgcggaa aacaggaatc
     3301 tcgacgggtg tttcatagcc cgccgcgctg aacttcaact ggacgatctc gcgatcggga
     3361 tcctggatcg cgatcagtag atcctggcgc agttccacag attttcccgc cagcaccagc
     3421 ggcaaggcga agtccgcctg cggtatggag acgccggagt tgggactgct tacctgcacg
     3481 cgcgcgttgc gggcatccgc cgcgcccttg ttcaacaccg tcaacttcag ccgcacttgt
     3541 ttgccggccg ttgcccaagg ggcctccacc acgcgccatc cggaaattgt caggatgggg
     3601 gccttggttt cattgatacc gatctcgtgg agtcctccat cgagggtgaa ctgcaaccgc
     3661 cccgacgaat ccgctttcat tgtgctgcgg cgcgcttccc cggtggacac gttcacgtcc
     3721 gtaatttcgt acgaggcttg cggccggtag atcgcatccg tcgtgatgcg gacgtttacg
     3781 gaggaaagca ggcgtcccga cggcacccat tcccgcacgc tggaacggaa gcccttcgct
     3841 gtaacgtttt cgatgagcgt gaagccaggc tgctgcctgt ccgtctgaac ggcgtatccc
     3901 cagacttcga attccggata gacctcgatg tggtgccaaa ccggcgggcg cgggagcgga
     3961 ttccggaagg cgttcatatg aaactgcagc gtcttcgcca tgccgggcgt gccgtgttcg
     4021 gaatcgaact cctcgtgctc gtgatggggc cgcacgaaat cccaaacggc gttcatgcgc
     4081 cggtgatacc agcggataaa gtccttcgac cccacgacga tcctggtgcg gagcccttcg
     4141 tagttccgat acatttcggt gtgccggtat tccgatggaa attctttcgg acctacgtaa
     4201 aattccgacg aacccatgaa attcgaaaca ctgcccacca ggtgcggata tttgccgccg
     4261 acccagtacg acatgaagcc gcccattgac aggccggccg tggcgcgatg atcgcgatcg
     4321 ggaatggttc gataattcga atcgatgtag cgaaccagtt ccgggaagta gagtgggaac
     4381 tggcgatgcg tctcgaccgg gctgatgtta taagggcgcg ggtagtcctc gccgggtgtg
     4441 cgtgggttgt agccgtccca ccggactacg atgaggtcgt tgcggctgac aaaggcggca
     4501 atgttgtcgc cgccatagtc gtcgcctgaa tcgtatccct tgccactgcg cggcggcttg
     4561 ttaaaacgct cgctccagcc gtggaaaaag tag
//
LOCUS       DASTMK010000091         8350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_100807, whole genome shotgun sequence.
ACCESSION   DASTMK010000091 DASTMK010000000
VERSION     DASTMK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100807"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..403
                     /locus_tag="VFQ79_03700"
     CDS             149..403
                     /locus_tag="VFQ79_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138784.1"
                     /translation="MSVTIEIPISDELLSSLDRKAREAGMNREEYLRALVARDLSGSK
                     TLDEVLGAFRDQVATSGMSDAQIDELFSTARDEAYRERNS"
     gene            412..903
                     /locus_tag="VFQ79_03705"
     CDS             412..903
                     /locus_tag="VFQ79_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEU0138785.1"
                     /translation="MTATQERPRVVFDCMVFLQGAARLHSPSGLCLLLAEHGVIELCV
                     SVDVLREIADVLRRPRVRAKFPSLTADVVEEFMRALNRMTTLFPDVPHEISFSRDPKD
                     EPYLNLVLRAQAHYLVSRDKDLLGLNIVNDSTGEKLRHRCPQLQILEPVSFIAALKEE
                     PRA"
     gene            complement(929..2011)
                     /locus_tag="VFQ79_03710"
     CDS             complement(929..2011)
                     /locus_tag="VFQ79_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0138786.1"
                     /translation="MPCDFSRRQLFYLAGMGTAGRLAAQQAPAAESQGLPVPVLPVNS
                     RSAVSLVKGEERRKIVCEALEAIDDQIRPVLKRKKYVIIKPNNVSTVRQLAATHADTV
                     RGILDYLAPRFKGPVVIAESSAGATLEGFENFGYNKVATEHRSQRVKLVDLNQEALYK
                     VIPLVNYDVHPIPVRLAARLMDPDAYTICAAILKTHNAVIATLSVKNMTLGAPLHQPP
                     GETSRWNDKRKYHEGVRVNHYNMMLTAQALKPYWGATLIDGFEGMEGNGPSSGTPVAS
                     RIAIASTDYIAADRIGVEAMGINPAWVGYLNFCWQVGLGQYDLAKIDVRGAKLEDVRR
                     QYRLHNDIERQLEWMGPMRDLPPKLG"
     gene            2079..3212
                     /locus_tag="VFQ79_03715"
     CDS             2079..3212
                     /locus_tag="VFQ79_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0138787.1"
                     /translation="MKSSSRRNFLTASLALPAAGLASQQPSAPSGALRYREIGKTGLK
                     VTSVSFGTMITSDPSVIERAADMGINYFDTARSYQNGNCERMVGAALKSRRKNLCLST
                     KSGAGTKEAALAHLETSLKELGTDWVDIWYLHAKSRPGDITDDLMEAQRIAKQQGKVR
                     FAGISTHAGFAELLPAVIKAKHFDVVLTSYNFTMDPEMAPLIESVRKAGIGIVAMKVM
                     AGGFRRLKPGDKLYSTFKKEGAMLAALKWVLRNPNVDTTIPSITDVDQLEENMEAMTV
                     PFGSSDEQVLKAQLDYIRPIYCRMCGRCDGTCSKGIPVADVLRFLAYAEGYGQFSLGR
                     ERFIELPLEVRDQRCSSCASCSVTCPNGVQVAQRLVRAQEMFA"
     gene            3209..4039
                     /locus_tag="VFQ79_03720"
     CDS             3209..4039
                     /locus_tag="VFQ79_03720"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEU0138788.1"
                     /translation="MKLLFLFLLAASMVMAQAPSCEIAPGWKQEGPARTFVPDTLFEY
                     MNGNAEGYLIYQFVRLNGITCKSGENEVVVDVFEMASPESAYGIFAANRDARQPIEKI
                     GMIGQVVPQRAVFAKGKYYVELAASSAADQSALLRSMAAEMEKRIEGGTELPPILSWF
                     PAEGLVADSIRLVPQSLLGFRMLRRGFLAQYDSGKAFIIPEDSPQQAAAVVAKFRERV
                     GNTQPAEIGDGGFKADDKYLGGLCVFSQGRYVAGFANMPAGEDPAAAAATIAARLPKP
                     "
     gene            4067..4846
                     /locus_tag="VFQ79_03725"
     CDS             4067..4846
                     /locus_tag="VFQ79_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0138789.1"
                     /translation="MPGALEGKTALVTGASKGIGKGIALELARAGCDVAVNYNSDRKG
                     AEVTVSEIRGLGRQACEIQANVGRRADVETLFADLRQRFSRLDILVNNAGTQVFKPLL
                     ELNEEEWDRVIDTNLKGCFLCTQQAAAWMKENGGGRIINIGSGCNKTPFPGLVSYTAS
                     KGGMEMLTKVAAVELGPHRIQVNCVAPGAIEIERTREEAGDYAGTWAKLTPLHRVGNP
                     QDVAKAVVFFASDAADFITGQTVWVDGGLFTKPQWPYDELS"
     gene            complement(4854..5600)
                     /locus_tag="VFQ79_03730"
     CDS             complement(4854..5600)
                     /locus_tag="VFQ79_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0138790.1"
                     /translation="MNPEGTRLRVLTYNIFIGTGMDKKIDLERTARVIRSASPDLVAL
                     QEVDRWARRSAGVDQPAELARLTGLNMAFGKASVREGSDEHGGDYGNAILTRLPINWY
                     ATRQLPFTPGFELRCVLEVELDWRPGGKNLPLRFFATHFDNASADDRLASAKLLVQLA
                     NQRPEALSILAGDLNAVPSSEPLGLLRQAWQLAGDGQEHPTFPADNPRRQLDYILFRP
                     AERWKVSEFRVLDEGTASDHRPVLAVLELL"
     gene            complement(5597..6316)
                     /locus_tag="VFQ79_03735"
     CDS             complement(5597..6316)
                     /locus_tag="VFQ79_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU0138791.1"
                     /translation="MAQLYAGTSGFAYPQWKPAFYPADVPAKNFLKAYSERLNCVEIN
                     YTFRRLVSASVLANWISQTAPGFVFAVKAHMRLTHVLRLRNAEEFTSAFLNSLNPLQA
                     AGRLGPVLFQLPPQFQCDADALAAYCEMLPRHLRFAFEFRHRSWLVEPVYDVLRQYNM
                     SLCVAESDKLEVPEVITADFVYYRLRKPEYSLSDIEEIGQRAEGLLKEGKDLYLIFKH
                     EDTPDGALNAERVLLRQAGNP"
     gene            6417..6587
                     /locus_tag="VFQ79_03740"
     CDS             6417..6587
                     /locus_tag="VFQ79_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138792.1"
                     /translation="MPRGASPKREREYEKLKRDFKKEGRYKGREEEVAARIVNKQRSQ
                     SGETKSRRHKKT"
     gene            6636..>8350
                     /locus_tag="VFQ79_03745"
     CDS             6636..>8350
                     /locus_tag="VFQ79_03745"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEU0138793.1"
                     /translation="MPRIRILAGMLLAGCCFAGVTRIEIKERTDVLGGRAFGSVGPYE
                     RIAATAHFAIDPKLPANRIISDVDLAPRNPDGLIEFSADLYVLRPRDPAKGNGTVLYE
                     VSNRGGRGLLSMFDLGASLADAATKEQFGDGFLLDQGFTLVWLGWQADVPQIEGRLRL
                     YAPRVQGVTGLLRAEFVVDEPVHTHSLADRNHIPYPVLDLKDPSLRLTVRDSVEGARQ
                     EVPRGEWDFADSGTLRAKNGFEPGRIYEFVYQSKDPILVGLGPAGVRDLISFFKYGGP
                     NTLLADQRRYTKQAMGFGTSQSGRFLRTFLYYGFNQDEKNRKVFDGVWAHVAGGGRGS
                     FNHRFAQPSRDGHPFMNMLYPTDIFPFSDLNQTDPVTGVTDGILRKAQEQGAVPKIFY
                     TNGSYEYWGRAASLIHTNLEGTQDAELASDTRIYLLAGTQHGPGRFPPAKNRTQNTAN
                     PADYRPLMRALLVSFQEWLKDGVEPPASEYPLIAKGQLVAVDKLRFPKLAGVEVPKYI
                     QQAYRADYGEEFRSAGIVTKEPPTINGKYTALVPQVDESGNEISGIRLPEITVPVASY
                     TGWNLR"
BASE COUNT         1778 a         2411 c         2568 g         1593 t
ORIGIN      
        1 cggacacaaa ggatggtggt cgaacgcata cgtatttgca tgaaagtact tattttagcg
       61 cagtatggcg gagcgcttcc aaggttccgg ggaacagggg cgcgaaaacc aaaggccttg
      121 cgggcataat gaatagaaga ggaagcccgt gagcgtcacg attgagattc ccatttcgga
      181 tgaacttctc tcgtccctgg accggaaggc ccgcgaggca ggtatgaacc gcgaagaata
      241 tttgagagcg ttggttgcgc gggatctttc cggctccaag acgctggatg aagtgctcgg
      301 cgcatttcgc gatcaagtgg ccacgagcgg catgtccgac gcccagatcg acgagttgtt
      361 ctcgacagcg cgggacgaag cataccggga gagaaattcg tagacgcctg aatgacagca
      421 acacaggaac ggccgcgggt ggtgttcgat tgcatggtgt tcctccaggg agccgcccgg
      481 ctacacagcc cctcgggact ttgtctgctg ttggccgagc acggtgtaat tgaactgtgc
      541 gtcagcgtgg acgtacttcg ggagattgct gacgtcctca gacggccgcg tgtccgcgcg
      601 aagttcccgt ctctaactgc tgatgttgtc gaggaattca tgcgcgcact gaaccggatg
      661 acaacgctct ttccagatgt gcctcatgaa atctccttct ctcgtgatcc gaaagacgag
      721 ccgtacctga atttggtgct gagagcgcaa gcgcattatc tcgtcagccg ggacaaggat
      781 ctgctcggcc tgaacatcgt gaatgattca accggtgaaa agctgcgcca ccgctgccct
      841 cagcttcaaa ttctggagcc tgtaagcttc atcgccgccc tcaaggaaga gccgcgcgcg
      901 taagtaagcg gaggacgtga cgagcgcgtc agcccagctt cggcggcagg tcgcgcatcg
      961 ggcccatcca ttccaactgg cgctcgatgt cattgtgcag ccggtattgg cggcgcacgt
     1021 cttccagttt ggcgccgcgt acgtcgattt tggcgaggtc gtattggccg agtcctacct
     1081 gccagcagaa gttgaggtag ccgacccagg ccggattgat gcccatcgcc tcgacgccga
     1141 tacggtccgc ggcaatgtaa tccgtggagg cgatggcgat gcgcgatgca accggcgtgc
     1201 ccgacgacgg gccattgcct tccatgccct cgaaaccgtc gatcagggtt gcgccccaat
     1261 acggtttcag ggcctgcgcc gtcaacatca tgttgtagtg gttcacccgg acgccttcgt
     1321 gatacttgcg cttgtcattc cagcgggacg tctcgccggg cggctggtgc aacggagccc
     1381 ccaaagtcat gttcttgacg gacagggtgg caatcactgc attgtgcgtt ttcaagatcg
     1441 cggcacaaat ggtgtaagca tcaggatcca ttaaacgcgc cgccagccgc accgggatcg
     1501 ggtgcacgtc gtagttcacc agcggaatca ccttgtacag cgcttcctgg ttcaggtcca
     1561 ccagcttcac gcgctgtgaa cggtgctccg tggccacttt gttatagccg aagttctcga
     1621 agccttcgag cgtagcgccc gccgacgact cggcgatcac aaccggtccc ttgaagcgcg
     1681 gtgcgaggta gtcgaggatg ccgcgcaccg tatccgcgtg tgtggccgca agctgcctga
     1741 cggtggatac gttgttcggc ttgataatga cgtacttctt gcgtttcagc accgggcgaa
     1801 tctggtcatc gatcgcctcc agcgcctcgc agacgatttt ccggcgctct tcccccttga
     1861 ccagcgaaac ggcggaacgg ctgttaaccg gcagcaccgg tacggggagg ccttgcgact
     1921 ccgcggcggg cgcctgctgc gccgccaaac gtccggcagt acccatcccc gccagataga
     1981 aaagctggcg gcgagagaaa tcacatggca tcgtcagacc tccaagtctt ataatagact
     2041 ttcgaatctg attatatttc atcttgcatg aggctgtcat gaaatcgtcc tcccgccgca
     2101 acttcctcac cgccagcctg gcactacccg cagcaggcct ggcttcccag caaccatccg
     2161 cgccgtccgg agcgcttcgc tacagagaga tcggcaaaac gggccttaag gtgacttcgg
     2221 tcagcttcgg caccatgatc acttccgacc ccagtgtgat tgagcgcgcc gccgacatgg
     2281 gcatcaatta cttcgatacg gcccgcagtt atcagaacgg caactgcgaa cgaatggtcg
     2341 gcgcagcctt gaagagcagg cgtaaaaacc tgtgcctgtc gacgaagagc ggcgcaggca
     2401 cgaaagaagc ggcactggca cacctcgaga ccagcctcaa agaactcggg acggactggg
     2461 tggatatctg gtacctgcat gccaagagcc gtcccggcga catcacggac gatttgatgg
     2521 aagcgcagcg gattgccaaa cagcagggca aggtgcgctt tgccggtatc agcacgcatg
     2581 cgggattcgc ggagctgctt ccggcggtca tcaaggcgaa gcacttcgac gtggtgctga
     2641 cgtcctacaa ctttacgatg gatcccgaga tggccccgct catcgagtcc gtgcgcaagg
     2701 ctggcatcgg cattgtcgcg atgaaggtga tggcgggagg attccggcgc ttgaagcccg
     2761 gtgacaagct gtattcgaca ttcaagaagg aaggcgcgat gctcgcggct ttgaagtggg
     2821 tgctgcgcaa tccgaacgtc gataccacca ttccgagcat aaccgacgtc gaccagcttg
     2881 aagagaacat ggaggcgatg acggtcccat tcggctcctc ggacgagcag gtgctgaagg
     2941 cgcagttgga ttatatccgg ccgatatact gccgcatgtg cgggcgatgc gacggcactt
     3001 gctccaaagg aataccagtc gcggatgtgc tgcggttcct ggcctacgcg gaaggctatg
     3061 gccagttctc gcttggccgg gagcgcttca tcgaactgcc gcttgaagta cgagaccagc
     3121 gttgttcgag ttgcgcctcc tgttccgtca cctgtccgaa cggagtacag gtggcacaaa
     3181 gactggtgcg cgcacaggag atgtttgcat gaagttgttg ttccttttcc tgctggccgc
     3241 atcgatggta atggcgcagg cgccttcgtg cgagattgca ccgggatgga agcaggaagg
     3301 accggccaga accttcgttc ccgacacgct gttcgagtac atgaacggca acgccgaagg
     3361 ctacctcatt taccagttcg tccgcctcaa tggaatcacc tgtaaatccg gagagaacga
     3421 agtggtggtg gatgtgttcg aaatggcctc gccggaatcc gcctacggca tctttgccgc
     3481 caatcgcgac gcgcggcaac ccatcgagaa gatcgggatg ataggccagg ttgtgccgca
     3541 gcgagccgtc ttcgccaagg gaaagtatta cgtggaactc gcggccagct ccgctgcgga
     3601 ccaatccgcc ctgcttcgtt ccatggccgc cgaaatggag aagcgcatcg aaggcggcac
     3661 ggaactgccg ccgatactga gctggtttcc cgccgaagga ctggtggcgg actccatccg
     3721 gctggtgccg caaagcctgc tgggcttccg gatgctgcgg cgcggatttc tcgcacagta
     3781 cgattccgga aaggcgttca tcatacccga ggattcgccg cagcaggcag ccgcggtggt
     3841 ggcgaagttt cgcgaaaggg tcggcaacac acaaccggct gaaatcggcg acggaggatt
     3901 caaggccgac gacaagtacc tgggcggatt gtgcgtcttc agccagggac gctacgtggc
     3961 cggattcgcg aacatgcccg ccggggaaga tccggcggcc gcggcggcaa cgatagccgc
     4021 acggcttccc aaaccataac ggcgccatcg acgctacaat caatccatgc cgggtgctct
     4081 ggaagggaag accgctctcg ttactggcgc cagcaaggga atcggaaaag gtattgcgct
     4141 cgaactggcc cgggccggat gcgacgttgc cgtcaactac aacagcgaca ggaaaggtgc
     4201 ggaggtcacc gtttccgaga tccgcgggct ggggcgtcag gcctgtgaga tccaggcgaa
     4261 tgtcggccgc cgcgcggacg tcgagaccct gtttgccgat ttgcggcagc gtttctcacg
     4321 cctggatatt ctggtgaaca acgccggcac acaggtattc aagcctctgc tcgagttgaa
     4381 cgaggaggag tgggaccgcg tcatcgacac gaacctcaaa gggtgcttcc tctgtacgca
     4441 gcaggctgcc gcgtggatga aagagaacgg cggcgggcgc atcataaaca tcggctcagg
     4501 ctgcaataaa accccgtttc caggcctcgt cagctacacg gcgagcaagg gcggtatgga
     4561 gatgctcacc aaggtcgccg cggtagaact ggggccgcat cgcatccagg tgaactgcgt
     4621 cgctcccgga gccatcgaga tcgaacgcac cagggaagaa gcgggcgatt acgcgggcac
     4681 gtgggcgaag cttacgccgc tccaccgcgt cggcaatccg caggatgtgg cgaaagcggt
     4741 ggtattcttt gcgagcgatg ccgcggactt catcaccggc cagacggttt gggtggacgg
     4801 aggactgttc acaaaaccgc aatggcctta cgacgaattg tcttgaggac ggcttacagc
     4861 aactccaaca ccgccagcac cgggcggtgg tcggaggcgg tgccctcgtc aagaactcgg
     4921 aattctgaca ccttccagcg ttccgcggga cgaaagagga tatagtcgag ctgccgccgg
     4981 gggttgtccg cgggaaaggt tggatgctcc tgcccatcgc ccgcgagctg ccacgcctgc
     5041 cgcaacaggc caagcggttc gctcgacggc acggcgttca gatcgcccgc cagaatggaa
     5101 agagcttcgg gccgttgatt cgccagttga acaagcagtt tggccgatgc gagacggtcg
     5161 tccgcgctgg cgttgtcgaa atgagtggcg aagaaccgca gcggaagatt ctttccgcct
     5221 ggccgccagt ccagttccac ttccagcacg cagcgcaatt cgaaacccgg cgtgaaaggc
     5281 agttgccgtg tcgcgtacca gttgatgggc aaccgcgtga gaatggcgtt cccgtagtcc
     5341 ccgccgtgct cgtcgctgcc ttcccgcacg gaggccttgc cgaacgccat gttcaagcca
     5401 gtcagccggg ccagttccgc aggctggtcc acccccgcgc tgcggcgcgc ccaacggtcc
     5461 acctcctgca atgcgaccag gtccggcgag gccgagcgga ttacgcgggc cgtgcgctcc
     5521 aggtcgatct tcttatccat gccggttccg ataaagatgt tgtaagtgag aacgcgaagg
     5581 cgtgtccctt ccggattcat ggattgcccg cctgccgcag gagcacccgt tccgcgttca
     5641 aagcgccgtc cggtgtgtcc tcgtgtttaa agatcaggta cagatctttg ccctccttga
     5701 gcaggccctc ggcccgctgc cctatctcct cgatatcaga aaggctgtat tcgggttttc
     5761 tcagccggta gtaaacgaaa tcggcggtga tcacctcggg aacttcgagt ttgtccgatt
     5821 cggcaacaca gagcgacata ttgtactgcc gcaagacatc atataccggc tcaaccagcc
     5881 aggaccggtg ccggaattcg aaagcgaagc ggaggtgccg gggcagcatc tcgcaatacg
     5941 ccgccaaagc gtcggcgtcg cattgaaact gcggcgggag ttgaaataaa accggtccga
     6001 gccgcccggc ggcctgcaac ggattcagcg aattgagaaa cgcgctcgtg aactcctcgg
     6061 cattccgcag cctgagcaca tgcgtcaacc gcatgtgcgc cttcaccgcg aatacgaagc
     6121 ccggcgcggt ctgggaaatc cagttcgcca gaaccgaagc cgataccagg cgccggaacg
     6181 tgtaattgat ttccacgcag ttcagccgtt cggagtaagc tttcaggaaa tttttcgcag
     6241 gtacgtccgc cggatagaat gcgggcttcc actgtggata ggcgaaccca gatgtgccgg
     6301 catagagttg agccatttgt tttcgttagc ttaaccaaaa cgggaccgcc gttcaaacgc
     6361 tgccggagat gtactggcag cccgtttgct caaaaagact atatggagga gcaggcatgc
     6421 caagaggagc aagtccgaaa cgggaacggg aatacgagaa gctgaagcgc gacttcaaaa
     6481 aagaggggcg ctacaaaggg cgcgaggaag aggtggcggc gcgtatcgtg aacaagcaga
     6541 gatcccagtc tggcgaaacc aagtcgagaa ggcataagaa aacataaagc actcttgagt
     6601 tgacggcggg cgggcaggcg aaatctaatg tgggaatgcc acggatacgt attctcgctg
     6661 ggatgttgct cgccggttgc tgctttgccg gtgtaacccg cattgaaatc aaagagcgca
     6721 cggatgtgct cggcggccgc gcttttggat ccgtgggacc gtacgagcgc atcgccgcca
     6781 ccgcgcattt cgccatcgat ccgaagctgc ccgcgaatcg catcatctcc gatgtcgatc
     6841 ttgccccgcg caacccggac ggactcatcg agttctccgc ggatctttac gtattgaggc
     6901 cgagagatcc cgccaaaggc aatggcaccg tgttgtacga agtttcgaac cgcggcggca
     6961 ggggattgtt gagcatgttc gacctcggcg cctccctggc agatgccgcc accaaggagc
     7021 agttcggcga cggctttctg ctggatcagg gcttcacgct ggtttggctt ggctggcaag
     7081 cggacgtgcc acaaatcgaa ggccggctgc gtttatatgc accgcgcgtt cagggagtca
     7141 ctggactgct ccgtgccgaa ttcgtagtgg acgaaccggt ccacacgcac tcgctcgcgg
     7201 accggaatca tattccctat ccggtgctcg acctgaaaga tccgtcgcta cggctgacgg
     7261 tccgcgacag tgtcgagggc gcacggcagg aagttccacg cggcgaatgg gatttcgccg
     7321 attccggaac attgcgagca aagaacggct tcgaaccggg aagaatttac gagtttgtat
     7381 atcagtcgaa agacccgatt ctggtggggc tggggccggc cggggtgcgc gatcttattt
     7441 cgttcttcaa gtatggaggg ccgaacacgc tgcttgccga tcagcgccgc tacacgaaac
     7501 aggcaatggg attcggcacg tcccaaagcg gccgtttttt gcggacgttc ctctactacg
     7561 gattcaacca ggacgaaaag aatcgaaaag tgttcgacgg cgtgtgggcg cacgtggcgg
     7621 gcggcggcag aggcagtttc aatcaccgtt tcgcgcagcc ttcgcgcgat ggccatccgt
     7681 ttatgaatat gctctatccg acggatatct tcccgttttc ggacctgaac cagaccgatc
     7741 cggtgacagg ggtaaccgac ggaattctcc ggaaggcgca ggagcagggc gccgttccaa
     7801 aaatcttcta tacgaatggc tcatacgaat actgggggcg ggcggcgtca ttgattcata
     7861 cgaatctgga agggacgcag gacgcggaac tcgcgtccga tacgcgcatt tacctgctgg
     7921 cgggcaccca gcatggtccg ggacgctttc cgccggcgaa aaacagaaca caaaacactg
     7981 cgaacccggc ggactacagg cccctgatgc gcgccctgct ggtaagtttt caggaatggt
     8041 tgaaggacgg cgtggaaccg cctgcgtccg agtacccgct catcgcgaaa ggccagcttg
     8101 tggccgtgga caagctccgg tttccgaaac tcgcgggcgt agaagttccg aagtacatac
     8161 aacaggccta tcgcgccgat tatggcgagg agttccgttc cgccggaatc gtcacgaaag
     8221 aaccgccaac gatcaatggc aaatacacgg cgttggtgcc gcaggtggat gagagcggaa
     8281 atgaaatttc cggcatccgc ctgccggaaa tcacagtgcc ggtcgccagc tacaccggct
     8341 ggaacctgcg
//
LOCUS       DASTMK010000092         6618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_548187, whole genome shotgun sequence.
ACCESSION   DASTMK010000092 DASTMK010000000
VERSION     DASTMK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548187"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFQ79_03750"
     CDS             complement(<1..320)
                     /locus_tag="VFQ79_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0138794.1"
                     /translation="MARFAGVDYLLIDSLFSDEELLVRQTAGQFVDERVIPLVRDCYR
                     DGRFPEELIPEMARLGFLGANLEGYGCAGMSNVEYGLIMQELERGDSGLRSFVSVQGA
                     LVMYP"
     gene            407..2410
                     /locus_tag="VFQ79_03755"
     CDS             407..2410
                     /locus_tag="VFQ79_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0138795.1"
                     /translation="MPFRVLLLTVLCPAVLLPADTLSQAYEALRSREYARAISLFETA
                     IKDDPGRVAVRKDLAYTYLKIGENELARDQFAEAMKIDTSDHHVALEYAFLCFETKRQ
                     ADARRVFDRVRKNGDAASRATAEQAFQNIDRPLAEGIARWEKALELSPDNFSAHRELA
                     ELAEQRDEPVLAAEHYEKAWRLRPDQRSLMLALGRVWKQAGRVEEANALLLAASRGAE
                     PRTAEAARELLPARYPYVYEFRRALTFDPQSVELRRELAYLLLEMGEKSQAEEEFDAI
                     VKAVPEDLLSSAQLGFLRLQRGDSESAMPLLDKVLKNADEELADRVRMMLKLPQTLRR
                     RPETPKRKISIEAKTMGERSYEAGYLKDALKYLKIANENDPLDFSVMLKLGWANNLLH
                     QDKEALQWFSLARSSPDPAISAEARRAHDNLRPALARVRTTAWLFPMYSSRWRDLFSY
                     GQIKTDVRLGKLPFRPYLSTRFIGDTRRTTGGIAPQYLSESSFIVGVGVATNAWHGAL
                     AWAEAGTAVSYLSGRKDTGGRFLPDYRGGVAFGRGFGMPIGGESHGTFFETNEDAVFV
                     SRFNNDLLFYTQNRAGYTLPAFGPLRVQLYWNANLTADARRQHWANFAETGPGLRFRF
                     DWMPESLVFSTGMLRGFYMMREGNPHGSHFTDFRAGFWYAFTR"
     gene            2394..4499
                     /locus_tag="VFQ79_03760"
     CDS             2394..4499
                     /locus_tag="VFQ79_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138796.1"
                     /translation="MRLPDRYTKPLPHGRGSVMEPRALASGIRYATKLLAVLMLAATA
                     WAGESVRYFQVVGDDPGSWPSILAAIGLQSGGTSIRRVYVIPPGETPEIQPWMERIDQ
                     GAIVVIEGASDFAASLGIRPSRSKVRIRNAQDLRAPGVPIIWEHTLDLPVFEAPEEAI
                     VFARERWGGAPLMLGIRRGKGAVLWVAASPGKNGHERFPYIPQALMDLGVEPPLRSSR
                     LWAFFDASYRLRVDLDYFAQQWRRAGIAGLHVAVWHYFEPDAERDAYLARLIEACHRN
                     GIVVYAWLELPHVSEQFWNRHPEWREKTALLQDAHLDWRKLMNMQNRECFREVAAGVR
                     ELISRFDWDGINLAELYFESLEGIDNPARFTPMNNDVRERFRRKHGTDPIALFREKPD
                     PSLRRKFLEFRAGLAQEMQEEWVGVLQSFRRAKPHLALTLTHVDDRFDTRMRDSIGAD
                     AARLMPMLERNDMTFLIEDPATVWHLGPQRYREIWLKYGAITTRRDRLGIDINVVERY
                     QDVYPTKQQTGTELFQLVHLCSGVFPRVALYFEKSILPQDVPLLASAAAHVKRLDQAE
                     SKLVVDSTYGIGIPWSGGALVDGKPWPALSDDTLWLPPGSHTVESGPAETPIRLLDFN
                     GGLLTAVTVQNGVEFSYDSDTRAIAVLDSAPALVQVDGAPHVTPTLANNGRYVLMLPR
                     GQHIVTVHSTTALSQRLEP"
     gene            complement(4511..5287)
                     /locus_tag="VFQ79_03765"
     CDS             complement(4511..5287)
                     /locus_tag="VFQ79_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0138797.1"
                     /translation="MNDNDLFSLAGKVAAVVGGGGVLAGEMAMGLARSGADIAILDYN
                     PAGAEARAAAVRALGRRALAVQVDATKKEDLSNALDRILAEFGRVDILVNAAGINSGT
                     PFFEITEEEWHRILDVDLKSVFIACQIFGKAMIESGNGGSIINISSASSGPPLSKVFT
                     YSVAKGGVNQITQFLAREFAPHRVRVNAILPGFFPAEQNRKLLTAERTQQILNHTPMN
                     RFGESSELVGAAVYLASEKASSFVTGSILRVDGGYGAMTI"
     gene            5453..5698
                     /locus_tag="VFQ79_03770"
     CDS             5453..5698
                     /locus_tag="VFQ79_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138798.1"
                     /translation="MDLYKTIRDLYEEKKRLDDLIASLEGMLLSDRPGTASKPRRRGR
                     KAMSPAERQAVSDRMRKYWAGRKKDDMPGDNLETTGS"
     gene            complement(5685..6356)
                     /locus_tag="VFQ79_03775"
     CDS             complement(5685..6356)
                     /locus_tag="VFQ79_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0138799.1"
                     /translation="MTLEEQLEKMRRDWDERARENARYYVNTAKTDWSDDEFFLSGEK
                     TVAEQILNDMINICQGKEPREMKVLEIGCGAGRITRALAGVFGEVHAVDVSGEMVKLA
                     RQSLQDRPNAFVYQNNGTDLTVIPGTDFDFAFSAIVFQHIPSREVIENYVREVHRLLR
                     PGALFKFQVQGDATLETNPGDTWLGVPFSDEQAVKMAGRCGFEPRHRHGAGEQYFWLW
                     FFKNP"
     gene            6445..6520
                     /locus_tag="VFQ79_03780"
     tRNA            6445..6520
                     /locus_tag="VFQ79_03780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6478..6480,aa:Ala,seq:cgc)
BASE COUNT         1355 a         1994 c         2002 g         1267 t
ORIGIN      
        1 gggtacatca cgagcgcgcc ctgaacggaa acgaagcttc gtaggccgga gtcgccgcgt
       61 tcaagttcct gcatgatgag accgtattcg acgttgctca tgccggcgca gccgtagcct
      121 tccaggttgg cgcccagaaa accgagcctg gccatttcgg ggatgagttc ctcggggaaa
      181 cggccgtcgc ggtagcagtc gcgcaccagc ggaattacgc gctcatccac aaactgcccg
      241 gccgtctggc gaaccagcag ctcttcgtcg ctaaacaacg agtcgataag gagataatcc
      301 acaccggcaa accgggccat aacagtgcaa ttatcccacc tgcgaacttt ccgccgctgg
      361 ccgcacctac aactagagga tacccatcag cgcccggaca ccagctatgc cctttcgcgt
      421 gctattgctg acagtattgt gtccggctgt gctgcttccg gccgatacgc tgagccaagc
      481 ctacgaagcg ctccggagcc gcgagtacgc gagggcgatc tcgctgttcg agacggcgat
      541 caaggatgac cctggccggg tggcggttcg caaggatctt gcctacacct atttgaagat
      601 cggtgaaaac gagctggcgc gcgaccagtt cgccgaagcg atgaagatcg acacttcgga
      661 ccaccacgtg gcactcgaat atgctttcct ctgcttcgaa accaagcggc aggcggatgc
      721 ccgacgggtt ttcgatcgcg tgcgcaaaaa tggcgatgca gcctcgcgag ccacggccga
      781 acaggctttc caaaacatcg accggcctct cgccgagggc atcgcacgct gggaaaaagc
      841 cctggagctt tcgcccgaca acttcagcgc gcaccgcgag ctggcggaac tggcggaaca
      901 acgcgacgaa cccgtcctgg ccgctgaaca ttatgaaaag gcgtggcggc tgcggccgga
      961 ccagcggtcc ttgatgttgg ccctgggtcg cgtctggaaa caggcgggcc gcgtggagga
     1021 agcgaacgcg ctgttgctgg ccgcatccag aggcgccgag ccgcgaaccg ccgaggccgc
     1081 ccgggaactg cttccggcgc ggtatccgta tgtctacgag ttccggagag ctctcacctt
     1141 cgatccgcaa agtgtggagc tgcggcgcga actggcttat ctcctgctag aaatgggaga
     1201 aaaatcacag gcggaagagg agttcgacgc cattgtcaaa gcggttcccg aagacctgct
     1261 ttcctccgcc cagcttgggt tcttgcgctt gcagcgcggt gattccgaat cggccatgcc
     1321 gctgctggac aaggttctca aaaatgcaga tgaagaactg gcggaccgcg tccgcatgat
     1381 gctgaagctt ccgcagacct tgcgccgccg gccggagacg ccgaaacgta agatctcgat
     1441 cgaagcaaaa accatgggcg agcgcagcta tgaggcaggc tatctcaagg atgcgctgaa
     1501 gtacctgaaa atcgccaacg agaacgatcc gctcgatttt tccgtgatgc tgaaactggg
     1561 gtgggcgaac aatctgctgc accaggacaa agaggcattg cagtggttca gcctggcgcg
     1621 cagcagcccc gaccctgcca tctcggcgga agcgcggcgc gcgcacgata acctgcggcc
     1681 cgcgctggcg cgcgtgcgaa ccaccgcctg gctctttccg atgtactctt cgcggtggcg
     1741 ggacctcttc tcgtacggcc agatcaagac cgacgtgcgc ctggggaaac ttccgttccg
     1801 cccgtatctt tccacccggt tcattgggga cacgcggcgc accacgggcg gtatcgcacc
     1861 gcagtacctt tccgaaagct ctttcattgt tggcgtcggc gtggcgacaa atgcctggca
     1921 cggcgcattg gcctgggctg aagccggcac cgctgtgagc tatctctcag ggcgcaaaga
     1981 taccggcggc aggttcctgc cggactatcg cgggggcgta gcgttcgggc gaggctttgg
     2041 catgcccatc ggaggagaat cccacggcac tttctttgag accaacgaag acgccgtatt
     2101 tgtcagccgg ttcaacaacg acctgctgtt ctacacacag aaccgcgcgg gttacacact
     2161 gccggcgttc gggcccttgc gagttcagct ctactggaac gccaacctga ccgctgacgc
     2221 gcgccgccag cactgggcca acttcgctga aaccggtccc ggcctgcgct tccgtttcga
     2281 ctggatgccg gaatcgcttg tgttctcgac cggcatgctg cgcggttttt acatgatgcg
     2341 ggaaggcaac ccgcacggat cgcatttcac cgatttccgg gcaggattct ggtatgcgtt
     2401 tacccgatag gtatacgaaa ccgctccctc acggtcgcgg ctcagtaatg gagccgcgcg
     2461 cgttagcaag cggtatccga tatgccacga aattgctggc tgtgctgatg ctggccgcga
     2521 ccgcatgggc cggcgaaagt gttcgttact tccaggtggt cggagatgat cccggttcct
     2581 ggccgtccat cctggcagcc atcggattgc agtccggcgg aacgagtata cggcgcgttt
     2641 acgttatacc tcccggcgag acaccggaga ttcagccgtg gatggaacgc atcgaccagg
     2701 gagctatcgt cgtaatcgaa ggcgcgtccg attttgctgc ctcgttggga atccgcccaa
     2761 gcagaagcaa agtacgaatc cgaaatgccc aggatctccg ggcgccggga gtgccgatca
     2821 tctgggagca caccctcgac ctcccggtgt tcgaggcgcc ggaagaagcg atcgtgttcg
     2881 cacgggagcg gtggggtggg gctccactga tgctcggcat acggcgtggc aaaggcgccg
     2941 tgctctgggt tgccgcgagt ccgggaaaga acgggcatga acgctttccg tacatcccac
     3001 aagcattaat ggacctcggt gtggaacctc cgctgcgttc ttcgcggttg tgggcgttct
     3061 tcgacgcatc ctaccgcctt cgagtagacc tcgattactt cgcgcagcag tggaggcgag
     3121 ccggaattgc cggacttcac gttgccgtgt ggcattactt cgaacccgac gccgaacggg
     3181 atgcttatct ggcaaggctg atcgaagcct gtcaccggaa tggaatcgtg gtctacgcgt
     3241 ggctcgagct gccgcatgtg agcgagcagt tctggaaccg gcacccggaa tggagggaga
     3301 agaccgctct gctccaggat gcccatctcg actggcgcaa gctcatgaac atgcagaacc
     3361 gggagtgctt ccgcgaagtg gccgcgggcg tgcgcgaact gatctcccgg tttgactggg
     3421 atggcatcaa cctggcggag ctctattttg aatcgctcga aggcattgac aatcccgccc
     3481 ggttcacgcc gatgaacaac gatgtccggg agcggttccg gcgcaaacac ggcacggacc
     3541 cgatcgcctt attccgcgaa aaacccgacc cgtctctccg gcgcaagttc ctggagtttc
     3601 gggccggcct cgcgcaagag atgcaggagg aatgggtcgg tgtgctccaa tcgttccgcc
     3661 gggcgaagcc gcacctcgcc ttgacgctga cgcatgtgga cgaccggttc gacacgcgga
     3721 tgcgcgattc gatcggcgcg gatgccgccc ggctcatgcc gatgctggag cgcaacgaca
     3781 tgaccttcct catcgaagat cccgccacgg tatggcacct cgggccgcaa cgttatcgtg
     3841 aaatctggct gaagtacggc gccatcacga cgcggcggga ccggctcggc atcgacatca
     3901 acgtggtgga gcggtatcag gacgtctatc ccaccaagca gcaaaccggg acagaactgt
     3961 tccagcttgt ccatctctgt tccggagtgt ttccccgcgt ggcgttgtac ttcgagaaat
     4021 cgatattgcc ccaggatgta cccctgctcg cctccgcggc cgcgcacgta aaacggctgg
     4081 atcaggcgga gtcgaaactg gtggtggatt caacctacgg catcggcatt ccgtggagcg
     4141 gaggggcgct ggtggacggc aaaccctggc ccgcccttag cgacgatact ttgtggctgc
     4201 cgccgggatc tcacacggtc gaatccggcc cggcagagac gccgatccga ctcctggatt
     4261 tcaatggagg gctgctgaca gccgttaccg tgcaaaacgg tgttgagttc agctatgact
     4321 cggacacgcg agccattgcc gttctggaca gcgcaccggc actggtgcag gtggatggcg
     4381 cgccgcacgt aacgccgacc ctcgccaaca acgggcgata tgttctgatg ttgccgcgag
     4441 gacagcacat cgtgacggta cattccacaa cggcgctgtc ccagcggctg gaaccgtaaa
     4501 ccttcggatg tcagattgtc attgcgccat agccgccgtc aacgcgcaga atagagccgg
     4561 tgacgaagga agacgccttt tcagaagcca gatatacggc ggcccccaca agttcgctgg
     4621 attccccgaa acggttcatc ggcgtgtggt tcaggatctg ttgggttctt tccgccgtca
     4681 ggagcttgcg attttgctcc gccgggaaga agcccggaag aatggcattc acacgcacgc
     4741 gatggggagc aaactcccgc gccaggaact gcgtaatctg attcacaccg ccttttgcca
     4801 cgctataggt gaagaccttc gacagaggcg gaccggacga cgcggaggag atgttgatga
     4861 tcgaccctcc attgccggat tcgatcatcg ccttgccgaa gatctggcaa gcaatgaaca
     4921 cactcttcag gtcgacgtca agaatgcgat gccactcctc ctccgtgatc tcgaagaagg
     4981 gagtgccgga gttgatgccg gcggcattga cgagaatgtc tacacgtccg aactcggcca
     5041 ggatccggtc cagcgcgttg ctcagatcct cctttttggt ggcgtccacc tgcacagcca
     5101 gtgcacggcg ccccagggcg cggacggctg cggcgcgggc ttcagcaccc gcgggattgt
     5161 aatcgaggat ggcgatatcg gcgccgcttc gtgcaaggcc catggccatc tctccggcca
     5221 ggacaccgcc tccgccgaca acagcggcta ccttgccggc gagcgagaaa agatcgttat
     5281 cattcatttc ggaactcacc ctcttttgta ccatgagtgt tccagaggcc tattcctgcg
     5341 ggttatatac tatctcgaaa tctggtacga atgccccggg cagatacaaa aatccagccg
     5401 gaaaagttca aatcctgtga cataatcagc agtgcacgcg acggagtcgc gcatggatct
     5461 gtataaaaca attcgggacc tttacgaaga gaagaaacgg ctcgacgatc tgatcgcctc
     5521 cctcgaaggt atgctgctat cggacaggcc gggaacagcg tcgaagccaa ggcggcgtgg
     5581 cagaaaggcc atgtcgccgg cagagcggca ggcggtctcc gatcggatgc ggaagtattg
     5641 ggccggccgg aagaaggacg acatgccggg ggataatctc gaaactacgg gttcttgaaa
     5701 aaccagagcc agaagtactg ctcgccggca ccgtgcctgt gccggggctc gaacccgcag
     5761 cggccggcca ttttcaccgc ctgctcatcg gaaaagggaa cgcccagcca agtgtcgccg
     5821 gggttggttt cgagcgtcgc atccccttgc acctgaaact tgaacaaggc gcccggccgg
     5881 agcagccggt gtacttcgcg cacgtagttc tcgatcacct cgcggctcgg gatgtgctgg
     5941 aacacaatcg cggagaatgc aaaatcgaag tccgttccgg gaatcactgt cagatcggtg
     6001 ccgttgttct gatagacgaa ggcattcgga cgatcctgaa gcgactgccg ggccagtttc
     6061 accatctcgc cgctgacatc aacggcgtgg acctcgccaa acacacctgc cagcgcacgg
     6121 gtgatccgcc cggctccgca accgatttcg agcaccttca tctcgcgagg ttctttcccc
     6181 tggcagatat tgatcatatc gttcaggatc tgctcggcga ccgtcttctc tcccgatagg
     6241 aaaaattcgt cgtcggacca gtccgtcttg gcggtgttga cataatatcg cgcgttctcc
     6301 cgggcccgtt cgtcccaatc gcgccgcatc ttctcgagtt gctcttccag cgtcatttgt
     6361 tgtagatatt gtagcttttt ccgtcccgcc agccggattc gagtgcgcca cacccgtgct
     6421 atatttggaa tgaatcctac gagtgggcgg ctagctcagt tgggagagca cggcgttcgc
     6481 aacgccgggg tcgtgggttc gagtcccatg ccgtccacca atcccaaacg atctgaaaat
     6541 ccaatgcgcc gggcgcatcg gcgagaatca aagcgggagg gaagagtgtg ccgggcggac
     6601 ttgaaaacag tgtaaata
//
LOCUS       DASTMK010000093         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_164698, whole genome shotgun sequence.
ACCESSION   DASTMK010000093 DASTMK010000000
VERSION     DASTMK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164698"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFQ79_03785"
     CDS             complement(<1..742)
                     /locus_tag="VFQ79_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138800.1"
                     /translation="MRLLLTGGTVVDGTGTPGVRADVLVENGRIASVGGLPENVEAER
                     IDCSGCIVAPGFIDIHSHSDLQAHQDKVEKARQGVTTEVVGNCSFSAFPVAEERFALH
                     EFANSIFCGSGDWGWRTAREYYADAAKSPGRVNPLSLVGHGTLRISEVGLKQGPLAPA
                     EMDAIEQRLSDCLAEGAVGFSTGLMYAPGSSAPFEELERLCRVVARHGKLYATHMRSY
                     FGGILESIDEQLELARRSGCRLQISHLQI"
     gene            848..>1945
                     /locus_tag="VFQ79_03790"
     CDS             848..>1945
                     /locus_tag="VFQ79_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU0138801.1"
                     /translation="MLNIQSCLERQKRLLSAMEQAHLDFVVLGYAKSVYYFTGVLVDS
                     SRPQVFAMDASGRSLLITNREPAQSAAGDTKIYTAYTIERPFGRTSMNDEAAAFLRSF
                     APPTAGSAGIDSEHLNAALAAAAGGRTQRNISPLLEQIRRRKDPDELECMRSAVTLAE
                     AGYKAVKERLEPGMTEFEAYSIIHEAMVRTAGTSVDLGGDFACGTRGIKAGGPPTDRK
                     VLAGDLYIFDLFPTRNGYSCDLCRTFAVGKPTELQQQAWSHVMEAHRIAEKAIRPGAS
                     GRDVYEAIRRHMEAFEPARGSFYHHAGHGLGLDAWERPLLTPGSEDVIEEGDVVACEP
                     GLYGENLQGGIRLEHNYLVGKSSTIPLDTFPM"
BASE COUNT          412 a          587 c          589 g          357 t
ORIGIN      
        1 cgatttgaag atgggagatt tgcagccggc agccgctgcg ccgtgcgagt tccagttgct
       61 catcaatcga ctccaggatg ccgccgaagt agctgcgcat atgggtggcg tacaatttgc
      121 catggcgggc gacgacgcgg cacaaccgct ccagttcttc gaaaggcgcg ctcgagccgg
      181 gcgcatacat gagaccggtc gaaaagccca cggcaccttc cgccaggcaa tccgacaggc
      241 gttgttcgat ggcgtccatt tccgccgggg cgagcggacc ttgcttcagg ccgacctcgg
      301 aaatgcgcaa cgttccgtgc cccacgagag agagcgggtt tacacgcccc ggacttttcg
      361 ccgcatcggc gtaatattcc cttgcggtgc gccagcccca atcaccggag ccgcaaaaga
      421 tactattggc gaactcatgc agggcgaaac gttcctccgc aacggggaaa gcggaaaagc
      481 tgcaattgcc gaccacttcc gttgtgacgc cctgccgcgc tttttccacc ttgtcctggt
      541 gcgcctggag gtccgaatgg ctgtgaatgt cgatgaagcc gggagcaacg atacaaccgg
      601 agcaatcgat ccgttcggcc tctacgtttt caggaaggcc gcccacggag gcgatccggc
      661 cattttccac caatacatcc gcgcgcacac caggggtgcc ggtcccgtcc acaacagttc
      721 cgcccgtcag aagaagtcgc attaacgcga ggagtaacac acggaggcga ttccggtcaa
      781 acgcgagcgt gccaccatac acgaagacga ggcggattag aatgcaggga aggagcgtcg
      841 cgcactcatg ctcaatatcc aatcctgtct agaacgccag aagcggctgc tcagtgctat
      901 ggagcaggcg catctcgact ttgtggtgct cggatacgcg aagagcgtct actattttac
      961 gggagtgctt gtggattcca gccggccgca ggtgttcgcg atggacgcct ccggccgcag
     1021 tctgctgatc acaaaccggg agcctgctca gtccgcggcg ggcgacacga aaatctatac
     1081 cgcttatacg atcgaacgcc cgttcggacg cacgtcgatg aacgatgagg ccgccgcgtt
     1141 tttgcgctcc ttcgcgcctc ccacagcggg cagtgccggc atcgattccg agcacctcaa
     1201 tgccgctctg gcagcggcgg cgggcggccg gacgcagaga aacatcagcc cgctgctcga
     1261 acagatccgg cggcgcaaag acccggatga actggaatgc atgcgcagcg ccgtcaccct
     1321 ggccgaagcc ggatataagg ccgtaaagga acggctcgaa cccggcatga cggagttcga
     1381 agcgtattcc attatccacg aggccatggt tcgcacagcc ggcacatccg ttgaccttgg
     1441 cggcgatttc gcctgtggta cacgtggaat aaaagctggt gggccgccga cggaccgcaa
     1501 agtcctggca ggcgatctct acatcttcga tctgttcccc acgcggaacg gttactcgtg
     1561 cgatctttgc agaacattcg ctgtaggcaa gcccacggaa cttcagcagc aagcctggtc
     1621 gcacgtgatg gaggcgcatc gcattgcgga gaaggccatc cggccaggcg ccagcggccg
     1681 tgatgtttat gaggcaatcc gccgtcacat ggaagccttc gaacccgcgc gcggttcctt
     1741 ctatcaccat gccggacacg gtctggggtt ggacgcgtgg gagcgcccct tgttgacgcc
     1801 tggtagtgaa gacgtaattg aagaaggcga tgtggtggcg tgcgagccgg gtttatacgg
     1861 cgaaaactta cagggaggca ttcgcctgga acacaactac ctggtgggaa agagttccac
     1921 cattccgctt gacacgttcc cgatg
//
LOCUS       DASTMK010000094         9633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_517213, whole genome shotgun sequence.
ACCESSION   DASTMK010000094 DASTMK010000000
VERSION     DASTMK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517213"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VFQ79_03795"
     CDS             <1..677
                     /locus_tag="VFQ79_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0138802.1"
                     /translation="EEERRTEMQAIQTPEQGSEQVKPSMSRLLSLRTTWGIILGKSLT
                     DPVWFFITDWFAIYLVSKGFRLENTLIGFWVPFLAADLGNFFGGGFSSYLIKRGWNVV
                     DARKFVILITGLGMALLAPAAFTSNFAALIGLFALSTFSYAAFSTMCLALPADLYPSS
                     TVASVSGMSGTGAGIGTIISTFLIGWVSDRYSFGPILVVASMVPLVATALVFLLVRRR
                     SQPAIS"
     gene            complement(670..1278)
                     /locus_tag="VFQ79_03800"
     CDS             complement(670..1278)
                     /locus_tag="VFQ79_03800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0138803.1"
                     /translation="MGVALTGCSDSTRDKAEATKAKAPVKPPDQYKATFETSKGNFVI
                     EVHREWAPRGADRFYELIQDGFYNESRFHRVVRNFVVQWGINKDPAVSRLWSNLRILD
                     DPPKQSNRKGTVAFAAAGPASRTTQVFINLRDNKQLDKNLFAPFGEVVSGMDVVESLY
                     NSYGEMPPRGNGPDPSLIETRGNEYLETKFPRLDYIKRASIN"
     gene            1369..2148
                     /locus_tag="VFQ79_03805"
     CDS             1369..2148
                     /locus_tag="VFQ79_03805"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU0138804.1"
                     /translation="MGLGYLVAVLLLLAGTPLYSETVMDESKLVDLTYALDENTVYWP
                     TGKPFEWRKEEWGMTAEGYWYAAANFSASEHLGTHLDSPIHFGKDQLTADQIPLSRLV
                     GKAVVLDVTSACNRNTDYRLTSSDIRAWEDRHGRIPGGAIVFVRTGWSKFWPDRKRYL
                     GSDKDGDTANLHFPGISAEAASILAERKIHGVGIDTASIDHGPSTDFPVHRILNGVGI
                     YALENVANLEQLPPTGASVFALPMKIKGGSGAPVRILAILP"
     gene            2234..3514
                     /locus_tag="VFQ79_03810"
     CDS             2234..3514
                     /locus_tag="VFQ79_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU0138805.1"
                     /translation="MDSTAFQVIAVLAIVVQGFLLFLFFFEPGIAYKIRRSPSVRLDS
                     DEFRRMIQVLTDAHIFSGNKIEVLTNGEIFYEAELEAIRAAQRTVNLEAYIFQRGEVT
                     RRFVKALAERAQAGVKVNVILDSIGSMTTWRSYFKELREAGGRVVYYNPLRWHHIVRF
                     NNRTHRELIIVDGSVGFIGGAGFADHWLKKKRNHPRWRDTMFRVQGEVVMGLQSTFAE
                     NWVEAAEEILTGDGYFSQCEAAGDCDAMVVESSPSAGRSTRARMLYQSLIASAQKCIQ
                     ITTPYFLPDWSARHEMVRAIKERGVKIRVIVPGKRSDHLLTRRSSRRLYGDLLKAGAE
                     IYEYEPAMIHTKTMTIDHLWSVVGSTNFDHRSFGLNDEVNLACSNRETAARIEEDFER
                     DLAASHRITYRQWKKRSVFERVQENLGWILERQQ"
     gene            3511..4272
                     /locus_tag="VFQ79_03815"
     CDS             3511..4272
                     /locus_tag="VFQ79_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0138806.1"
                     /translation="MSGSNLRIATYNVHKCRGLDGRLRPDRIVRVLREMEADVIALQE
                     VLSVDGHKFESDQPRFLAEQLGFHWALGENRRLNGGAYGNVVLSRFPLHQHCNYDISV
                     HGRESRGCLRTDVVLGDGHLLHVFNVHLGTSFVERRQQARKLIAGTIVLNGHKRGPRV
                     VLGDFNEWTRGLATRMLSAELESIDIRSHLRKARTYPGIFPFMHLDHIYHDPDLQLQK
                     LILHRSRTALVASDHLPLIAEFQLSRWTGGRNGSS"
     gene            complement(4267..7233)
                     /locus_tag="VFQ79_03820"
     CDS             complement(4267..7233)
                     /locus_tag="VFQ79_03820"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEU0138807.1"
                     /translation="MKQRKYIKSTGRFSTCLIAVIVASASSLNAQVPQPSVDILIPSR
                     MPAGAPTFTMLVGGTGFSPNTAVWWWDGTTATQLTVTSRISATELQVTIPESLLLLPG
                     MAEVRVSEGVTGPQSNSYSFFITTAPLIRSLDPPSAIAGGPDFTLRIIGERFDCSSGM
                     ASAKLVAKTASAPIYQEVRWNGNVLPAVCGSPTELQVAVPAALIATVGVAAIEVVDFS
                     TESQSNTVEFAIVPPGLPMPVITSLNPQFGIAGSGAVSLVIGGSGFASGAVVRWNSTA
                     LTPTSVTSTQIQVLIPAYLLATRTTAYITVENGVDIVSNALPFVVDLRPLQLNPGALP
                     DGNVGTPYSQQFSASGGDGRYSFALRQGVLPEGLRFDAATATIGGTPAREGRFSFSIS
                     VSDFSNSILSQLYTLVIAPSTLTITTTSVAEGQAGVPYEQRFQATGGTGPYNWSFTDA
                     GTPPPGLGLSVTGILSGRPSRLGTYVFGVRVTDSTGRSVSREYTMLVRSAITISSLPP
                     GEVGRNYSATLTATGGEPPYSWSLGGGLLPDGLSLSPGGTVTGAPTRQGGFTFTIRVS
                     DANNQSASRDFTVDVSLPLGIGTEGPFTGRIGTPFSQTITAAGGSTPYAWSLVSSGFS
                     GLTIDAATGTLAASPSSPGTYSFTVEVRDASDRVATKTFSARIESDITIGPGTLPAGT
                     AGSAYSTTLSASGGMPPYAWSASGLPGGLSVDPASGVLSGTPGGIGSFPVTITAQDAR
                     GIQGIRELVFSVGLPQLSGLNITIPQTVPPDTQPPLGLEIGQPFPVDLDGEMTLTFAP
                     QAVTSSPGLEDDPAIQFSTGGRTLSFRIPAGSTMASFGSGQPAIQVGTVAGVITLTVR
                     LRAGTTDVTAPPLPTRTIRIEATPPVIRSVRVTRTATGYEVTVNGYSTSREVLRARFR
                     FSPAAGASLSTQEATVDVASFFQTWYASSASTSFGSQFTFTMPFTVQGDLGAVRSVSV
                     MLTNSAGNSQDVSGSL"
     gene            complement(7238..8197)
                     /locus_tag="VFQ79_03825"
     CDS             complement(7238..8197)
                     /locus_tag="VFQ79_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138808.1"
                     /translation="MNHGLILLLAALLITGASGAESTSMRGPVSGYVFNSESASVRPV
                     LGMPGSSTLGEPLPSGTAIGRASISTEQNFAIVSIGESRTPAVLNLGDPLGEPVTIQG
                     LAPGDYSVALSPSGKAAALLEAESGTIHIVERIASAPGVAAPIRVPGASHPSRIAISD
                     DGARLLVVTRDGDSDVLTLLDNGGNVLSSTRLGEVAAAEFLFDSYDALVADRGRRELL
                     LTGAAGIRLLLDSSVLVEPVAVAASRHNRRAVVADAKSQAVVSVGLNGEAVLVTACPC
                     EVSGLQRFQGNAVFLLTSASSKPMWVFDGDAASPRVVFVPVME"
     gene            complement(8215..8841)
                     /locus_tag="VFQ79_03830"
     CDS             complement(8215..8841)
                     /locus_tag="VFQ79_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0138809.1"
                     /translation="MANKLVFSPPGRLLAMLLISIFSSGIPSAAQPAQAAGKLNIVVI
                     EGDGAINNIRQRVGREPIVQVEDENRKPVGGAIVTFLLPNQGASATFADGGRTLSVVS
                     DEQGRAIARGLQPNSIEGKYEIRVTASKDGNTASTTISQANALAAGAGAAAGGGLAAK
                     WIAVIAVAAAAAVAGGVVAARDNGNGNNRPSVTPTVITPGTPSVGAPR"
     gene            8990..>9633
                     /locus_tag="VFQ79_03835"
     CDS             8990..>9633
                     /locus_tag="VFQ79_03835"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEU0138810.1"
                     /translation="MFYRFAVLLAGVAALSPVPVFSQSSNSLSARELFYTPPPKAQPD
                     RVSPKAPSRKNAPAKADIAKADSPVVPQRAPRKPVEPERSQDEAGLVAVSHKPHFPLG
                     LRYSVLRSADGVNYSETDAGTAFRSGDKLKISLESNDTAYLYMVARGSSGTWSVLFPN
                     PEVARGSNIVAPGRTYEMPSGGRIVFDEQPGTEKMFIVLSRRPEPNLEELIYSL"
BASE COUNT         1992 a         2815 c         2929 g         1897 t
ORIGIN      
        1 acgaagaaga gcgccggacc gagatgcagg cgatccaaac gccggagcaa ggttccgagc
       61 aagtcaagcc cagcatgagc cggctgctct ctttgcgcac cacctgggga atcatccttg
      121 gcaaatcgtt gaccgacccc gtgtggttct tcattacgga ctggtttgcc atttacctgg
      181 tttccaaggg attccgtttg gaaaacacgc tcatcggatt ctgggtcccg ttccttgccg
      241 cggatctcgg gaatttcttc ggcggcgggt tctccagcta tctgattaaa cgaggctgga
      301 acgtagtcga tgcgagaaag ttcgtcattc tgatcacggg gctcggaatg gcactgctgg
      361 caccggcagc atttacttct aatttcgccg cccttattgg tttgttcgcg ctctccacgt
      421 tcagctacgc ggccttctcg acaatgtgtc tggctcttcc agccgatctg tatcccagca
      481 gtaccgtcgc ctccgtcagc ggaatgagcg gaacaggcgc cggtatcggc accatcattt
      541 cgacatttct gatcgggtgg gtctcagacc gctactcgtt cggccccatc ctggttgtgg
      601 cgagcatggt gccgctggtt gcaacggctc ttgtgtttct gctggtccgg cggaggtccc
      661 agccggccat cagttgatgg atgcccgttt gatgtagtct agccggggaa acttcgtttc
      721 cagatactcg ttgccccggg tctcaatgag gctcgggtcc gggccgttgc cccgcggcgg
      781 catctcgccg tacgagttgt agaggctctc gacgacatcc attccggaga cgacttcgcc
      841 aaagggagcg aagaggttct tgtccagttg cttgttgtcc cgcaggttga taaagacctg
      901 cgtcgttcgc gatgccggtc ccgcggccgc aaacgccacc gtacctttgc ggttgctctg
      961 ttttggagga tcgtccagaa tccggagatt gctccagagc cgcgacaccg cgggatcttt
     1021 attgatcccc cactggacaa cgaagttgcg caccacgcga tgaaaacggg attcgttata
     1081 aaagccgtcc tgaatcaact cgtagaaacg gtccgcgccg cgcggcgccc actcgcgatg
     1141 cacctcgata acaaaattgc ctttcgaggt ctcaaatgtc gctttgtact ggtccggcgg
     1201 cttcacggga gcctttgcct tcgtcgcctc cgctttgtcg cgcgtggaat ccgagcagcc
     1261 cgtcaaagca acgcccataa acagaattag tgttccagaa atgtcacggc cgcgaatcaa
     1321 ttgagtgtct cccggaatta ctttaccatc caacttgggg ggtggcggat gggtctggga
     1381 tatcttgttg ccgtattgct attgctggcg ggcacaccac tgtattctga aacagtgatg
     1441 gatgaatcga agctcgtaga cctcacttac gctctggacg agaatacagt ctactggccc
     1501 acgggaaaac cgttcgagtg gcgaaaggaa gaatggggca tgacggcgga gggctactgg
     1561 tacgccgcgg caaacttctc tgcgagcgag cacctgggca cgcacctgga cagccccatc
     1621 catttcggaa aagaccaact cacagcggac cagattccgc tgtcgcgact ggttggaaaa
     1681 gcggtggtat tggacgtgac cagtgcctgc aaccggaaca cggattaccg cctgacaagt
     1741 tcagacatcc gcgcatggga agaccggcat ggacgcatac cgggcggagc aatcgttttc
     1801 gtccggaccg gctggtcgaa attctggccg gatcgcaaac gctacctcgg aagcgataag
     1861 gacggcgata ctgccaactt gcattttcca ggcatttccg ccgaggccgc ttcgattctg
     1921 gcggagagga agatccatgg agtcgggatc gatacggcga gcatcgatca cgggccctcc
     1981 accgattttc cggttcaccg cattctgaat ggagttggaa tctacgcact ggagaacgtc
     2041 gccaatctcg aacaactgcc gcctacaggc gcctccgttt tcgcgttgcc gatgaaaatc
     2101 aaaggcggtt ccggggcacc ggttcgcatc ctggcgattc tgccctgagc agctccacgg
     2161 cacggattcc cggcgtttta gataccttga ggagccggtc tgcatctaca ataagtagtg
     2221 acagcctgcc cagatggatt cgacagcatt ccaggttatt gctgtacttg cgatcgtcgt
     2281 gcaaggcttt ttgctgtttc tgtttttctt cgaaccggga atagcctaca aaatccgccg
     2341 gtcgccatcg gtgcgcctgg attccgacga gttccggcgc atgatccagg ttttgacgga
     2401 tgcgcacatt tttagcggga acaagatcga ggttttgacg aacggcgaga tcttctacga
     2461 ggcagagttg gaagccattc gcgccgctca gcgcaccgtc aatctggagg cctacatttt
     2521 ccagcgcggc gaggtgaccc ggcgttttgt caaagcactg gcggaacgtg ctcaggcggg
     2581 cgtcaaggtc aacgtaatcc tggattcgat cgggagcatg accacatggc gcagctactt
     2641 caaggaactc cgggaggcag gcggacgagt tgtctactat aacccgctgc gctggcacca
     2701 catcgtgcgg ttcaacaaca ggacacaccg cgaattgatt attgtcgatg gctcggtggg
     2761 cttcatcggc ggcgcggggt tcgcggatca ctggctgaaa aagaagagaa atcatccacg
     2821 gtggcgcgac acgatgtttc gcgttcaggg tgaagtggtg atgggcctgc aatccacgtt
     2881 cgccgaaaac tgggtggagg cggccgaaga aatccttacc ggggacggct atttttccca
     2941 gtgcgaggct gccggcgatt gcgacgcgat ggtggtggaa agctctcctt cggccggccg
     3001 ttccacccgg gcacggatgt tatatcagtc gttgatcgct tccgcacaga agtgtattca
     3061 gatcacgacg ccgtattttc tgccggactg gagcgcgcgg cacgaaatgg tccgtgcgat
     3121 aaaggagaga ggggttaaga tccgggtgat cgtgcccggc aagcgcagcg atcacctgct
     3181 gacgcgacgt tccagccgca ggctttacgg tgatctgctg aaggcaggcg ccgagattta
     3241 cgagtacgaa ccagcaatga tccacaccaa gaccatgacc atcgaccacc tttggagtgt
     3301 ggtcggatct accaacttcg atcaccgttc atttggcttg aacgacgagg tcaacctcgc
     3361 ctgctcaaac cgggagacag ccgcccgcat cgaagaggat ttcgaacggg atctggcggc
     3421 gagccatcgc attacatacc gccaatggaa aaaacgctcc gttttcgaac gcgtccagga
     3481 aaacctcggc tggatactgg agaggcagca atgagcggga gtaacctgcg catcgccacc
     3541 tacaatgtgc ataagtgccg gggattggat gggcgccttc gcccggaccg cattgtgcgg
     3601 gtgctgcgcg agatggaagc cgacgtcatt gcgttgcagg aagtcttgag tgtggatggc
     3661 cataagtttg aatccgacca gccccggttc ctggccgaac agctcggttt ccactgggct
     3721 cttggagaga atcgcaggct gaacggcggc gcctatggaa acgtagtgct cagccggttt
     3781 ccgctccatc aacactgcaa ttacgacatc agcgtccacg ggcgcgagag ccgcgggtgc
     3841 ttgagaacgg atgtcgtcct gggcgacggc cacctgctgc atgtcttcaa tgtgcatctt
     3901 ggcacgtcct tcgtggaacg ccggcagcag gcgcgaaagc tcatcgcggg tacaatcgtt
     3961 ctgaacggcc acaagcgggg accccgtgtc gtgctgggag acttcaatga atggacccgg
     4021 ggcctggcaa cccggatgtt aagcgcggaa ctcgaaagca tcgacattcg cagtcacctt
     4081 cgcaaagcca ggacgtatcc tggaatcttc ccgttcatgc acctggatca tatctaccac
     4141 gacccggacc tgcagcttca gaagctgatc ctgcaccgca gccgtacagc actggtcgcc
     4201 tcggaccacc tacccctaat cgccgaattc cagctcagcc gttggacagg cggccgcaac
     4261 ggctcgtcat aagcttccgc tgacgtcttg cgaattgccg gcggagttcg tcagcatcac
     4321 cgaaaccgat ctgacggccc ccaggtcgcc ctgcaccgtg aaaggcatgg tgaaggtaaa
     4381 ctggcttccg aaactggtgg aagcggagct tgcgtaccac gtctgaaaga agctggcaac
     4441 atcgacggtc gcttcctggg tgctcaggct tgctcccgcg gccgggctga agcggaagcg
     4501 ggcgcgcaac acctcacggc ttgtagagta accgttcacc gtcacctcat atccggtagc
     4561 ggtccgggtt acacggacag accgtatcac cggcggtgtt gcctcgatgc gaattgtgcg
     4621 cgtaggcagc ggaggagccg ttacgtcggt tgtgcccgcg cgcagccgca ccgtcagcgt
     4681 gatgacaccg gcaacggtcc cgacctggat agccggctgc ccggacccga aactcgccat
     4741 cgtgcttcct gcaggaatac gaaacgaaag cgtacggccg ccggtggaga actgaatggc
     4801 tggatcgtcc tccagaccgg gtgagctcgt gacggcttgc ggcgcgaagg tgagagtcat
     4861 ctcgccatcg aggtccaccg ggaatggttg tccaatctcg agtcccaatg gcggctgggt
     4921 atcgggcggt accgtttgcg ggatggtaat attgaggccg cttaactgag gcaggccgac
     4981 ggaaaaaacc aactcgcgga ttccctggat gccccgcgca tcctgtgcgg tgatggtgac
     5041 ggggaaggat cctatgccgc cgggagttcc ggagaggacg ccggatgctg gatcaacgct
     5101 caggccgccg ggcaggccgg aggccgacca cgcgtaaggc ggcattccac cgctcgccga
     5161 caacgtcgtc gagtatgccg aaccagccgt gccggccggg agcgtgccgg gaccgatagt
     5221 gatatcggac tcgatgcggg cggagaacgt ttttgtagcc acgcggtccg aggcgtcgcg
     5281 aacttccaca gtgaagctgt aagtgccggg cgaggacgga ctggccgcaa gcgtgcccgt
     5341 tgccgcatcg atcgtcagac cggaaaaacc ggacgatacg agcgaccacg catagggcgt
     5401 gctgcctccc gcagccgtga tggtctgcga gaaaggtgtt ccgattctgc ccgtgaacgg
     5461 gccttcggtg ccgataccaa gaggaagcga aacgtcgact gtgaagtccc ggctcgccga
     5521 ctggttgttc gcgtccgaca ctcgtatcgt gaaagtgaag cccccctgcc gtgttggggc
     5581 tccggtcacg gtgcccccgg gggaaagact caggccgtcc ggcaacagcc cgccgccgag
     5641 cgaccacgag tagggtggtt cgccaccggt ggctgtcagc gtcgccgaat aatttcttcc
     5701 cacctcgccc ggcggcagag acgaaatcgt gatcgccgat cggacgagca tcgtgtattc
     5761 gcgcgacaca ctgcggcccg tggagtcggt gactcgaacg ccaaagacgt atgtcccgag
     5821 ccgggatggc ctgccggaaa gaataccggt tacggacagg cccagtcccg gaggcggcgt
     5881 tccggcatcg gtaaagctcc agttgtaggg accggtaccg cccgtcgcct gaaatcgctg
     5941 ttcgtaaggc acgccggcct gaccctccgc aaccgatgtg gtagtgatgg tcagcgtgga
     6001 gggcgcaatc accagcgtgt agagctggct gaggattgaa ttcgagaaat ccgagacaga
     6061 aatggagaac gaaaaccggc cttctctcgc cggtgttccg ccgatagtcg ccgtcgccgc
     6121 gtcaaaccgg agcccctccg gtagaactcc ctgacgcaaa gcaaaggaat aacggccgtc
     6181 cccgcccgac gcggaaaatt gctggctgta cggggttccc acgtttccat ccggcagcgc
     6241 cccggggttc agttggagag gccgcaggtc gaccacgaaa ggaagagcat tcgatactat
     6301 gtcgactccg ttttcaaccg tgatgtatgc ggtggtcctg gtggcgagga ggtaggccgg
     6361 aatcagcacc tgaatctgag tggaggtgac ggaggtgggc gtcagggctg tgctattcca
     6421 tcgcaccacc gctcccgaag caaatccgga gccgccgata accaggctga ccgctcccga
     6481 gccggctatt ccgaattgcg gatttagcga ggtgatcacc ggcattggca gcccgggagg
     6541 gacaatcgca aactcgacgg tgttggactg cgattcggta gagaagtcca ccacttcaat
     6601 ggccgcaacg ccaaccgtgg cgatcagcgc cgccgggacc gctacctgta gctcggtcgg
     6661 gctcccgcag actgccggta atacattgcc gttccagcgc acttcctggt aaatgggcgc
     6721 gctcgccgtc ttcgcaacga gtttcgccga cgccataccc gaggaacaat cgaaccgttc
     6781 tccgatgatt ctgagcgtga agtccgggcc gcccgctatc gcactgggcg ggtcgagact
     6841 acgaatcagt ggcgcagtgg tgatgaaaaa tgagtaggag ttcgactgcg gcccggtcac
     6901 gccttccgat acgcgaacct ccgccatccc gggtagaagg agaagggatt ccggtatagt
     6961 cacctggagc tctgtagccg agatccgtga ggtgaccgta agctgcgtcg ccgtagttcc
     7021 atcccaccac caaacggccg tattcgggct aaagccggtg ccgccaacca acatcgtaaa
     7081 cgtgggtgcg ccggcaggca tccgggacgg tatcaggatg tcgacggagg gttgcggcac
     7141 ctgcgcattc aacgaacttg cggacgcaac gatcacggcg atgagacaag tagaaaaacg
     7201 gccggtgctt tttatgtatt tccgctgctt cacactctca ctccattaca ggcacgaaga
     7261 caacacgtgg gctggccgcg tctccatcga agacccacat cggtttcgag gaggcggacg
     7321 tcagcagaaa cacggcgttc ccttgaaacc gttgaaggcc tgaaacctcg cacgggcacg
     7381 ccgtaacgag aaccgcttcg ccgttcaggc cgaccgagac cacagcctgg gatttggcgt
     7441 cggcgacaac ggcgcgccgg ttgtggcgcg aagcagcgac ggcaaccggc tcgacaagga
     7501 cggaggagtc gagtaataac cggatgcccg cagcgcctgt taacagcagt tcccggcgtc
     7561 cccggtccgc aaccagcgca tcatagctat cgaacaggaa ttcggcggcg gcaacttcgc
     7621 ccaatcgtgt tgagctaagc acattgccgc cattgtccag aagcgttaga acgtcggagt
     7681 ccccgtcgcg tgttactaca agcaatcgcg ctccgtcatc ggagatggca atcctgcttg
     7741 ggtggctagc gccgggaaca cgaatgggag cggctactcc cggtgccgaa gcgattcgct
     7801 cgacgatatg gatcgtgcca gactccgctt ccaacagtgc cgcggctttc ccggacgggc
     7861 tgagcgcgac cgagtaatcc cctggcgcca aaccctggat cgtaacgggt tcgccgagcg
     7921 gatcgcccag attcagcact gccggcgttc tgctttcgcc aatagatacg atggcgaagt
     7981 tctgctcggt ggagattgag gcacgaccga ttgccgtccc ggatggaagc ggctctccaa
     8041 gagtagagga tcccggcatt ccgagaacgg ggcgcacgga tgcggattca gagttgaata
     8101 cgtagcccga gacgggtcca cgcatggagg tggattccgc gcccgaagcc ccggtaatga
     8161 gcaacgccgc cagaagaaga atgagtccgt ggttcatctg tgaattcctc gagactaacg
     8221 gggcgctccg acggagggag ttcctggagt aataaccgtc ggcgtgacgc tgggccggtt
     8281 attcccgttg ccgttgtccc gcgcggcaac cactcccccg gcaaccgcag cggccgctgc
     8341 taccgcgatc accgcgatcc atttggccgc aagaccaccg cctgccgccg ctccggcgcc
     8401 ggcggcgagt gcgtttgcct ggctgatcgt cgtactggcg gtattgccgt ccttcgaagc
     8461 ggtcacccgg atctcgtatt tgccttcaat ggagttcggc tgcaggcccc gcgcgatcgc
     8521 tcttccctgc tcatcgctga ctacggacaa cgtacgcccg ccgtcggcga atgtcgcgct
     8581 ggcgccctga ttagggagca ggaaggtcac gatggcgccg cccaccggct tgcggttctc
     8641 gtcttccacc tggacaatcg gctcgcgccc gacccgctgg cgtatgttgt tgattgcccc
     8701 gtcgccttca atcactacga tgttcagctt tccggctgcc tgtgctggtt gtgcggcgga
     8761 cggaatgccg gatgagaaaa tcgaaatcaa cagcattgcc agcaagcggc ctgggggaga
     8821 aaagaccagt ttatttgcca tcccgaaccc tccgttcgaa tgggggatca atgaaaaatg
     8881 ctacctcaaa tgggttgtat aaccaaagag gggtactgtc agtactgaaa ctgatccaaa
     8941 atgaagctgt aaactaagga acgacgcgat aagaatcggg gagggcagta tgttctatcg
     9001 tttcgcagtt ctgttggccg gtgttgcggc gctgtcgccg gtgccggttt tttcgcaatc
     9061 gtccaattcg ctctccgcgc gggaattgtt ttatacgcct cctcccaaag cacagccgga
     9121 ccgtgtttcg cccaaagcgc cctccaggaa aaatgcgccc gcgaaagcgg atattgccaa
     9181 ggcggacagt ccggtggttc cgcagcgcgc cccacgaaag cctgttgaac cggaacggtc
     9241 acaggatgaa gccgggctgg tagctgtttc ccacaagccg cattttccgc ttggtcttcg
     9301 ctacagcgtt ctcagaagcg ctgacggcgt caactacagc gaaacggatg cgggcacggc
     9361 atttcgatcg ggcgacaaac tgaagatctc gctggagagc aacgatacgg cctatctgta
     9421 tatggtggcg cgcggctcca gcgggacctg gagtgtgctc tttccgaatc cggaagtggc
     9481 gcgcgggagc aacatcgtcg cccccggacg gacttacgag atgccatcgg gaggacgtat
     9541 cgttttcgac gaacagcccg gcacggaaaa gatgttcatc gtactttcac gccgtccgga
     9601 accgaacctg gaagaattga tttattctct gag
//
LOCUS       DASTMK010000095         5217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_581067, whole genome shotgun sequence.
ACCESSION   DASTMK010000095 DASTMK010000000
VERSION     DASTMK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581067"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="VFQ79_03840"
     CDS             <1..1846
                     /locus_tag="VFQ79_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138811.1"
                     /translation="MGATSGINGQLGLHGVDPEAFPIIIVPGLSNLGAGSGRSTYPID
                     TYHFLNNVTWIRGSHFIKTGVEFRYGGQTNTNKDLAGGQFRFGERITGSGLAELLLGR
                     AESAEIRSFEDISARIDNYGAFIQDDWKVSRNLTLNLGLRWEMDTPRWDKDNRMNSFD
                     LKAINPVCNCPGVITFAGLDGRSKYAHDFDKNNFGPRFGFAWNAMNRMVIRGGYAVHY
                     NGAYHSGIAGDIIAGFSNEASFSSPDGGITPAVLLRNGMPAIPAGEPLTPAFGAVPIG
                     ENPRYAPAFIQQNHVNGYAHQWNFGVQRELPANMLFEIAYLGKAGHKLGGDEYNLNVI
                     PLVNGRGPDVQRQTDRPFPQFDNVQHKFPPWGNSIYHAMNAKLEKRFSHGLNFLMNYT
                     FSKFLDDVEPKGELGGSGLDTGITHPELRRFDRAPSGNDIRHRYIVSGVYELPFGKGR
                     RWGIGNSVLDHIAGGWGLGVIAELRSGAPIGVIEQTNRTNTYSTNVRPNLLHDPGLSR
                     DRTRAEKLAQWFDTTAYAAPAVGEFGNAPRTIAGLGPGYFGLDASVNKTWSLTERYRL
                     MYRADFYNAPNYPVFAAPNRSRGSGNFGRITGTSGTGRQIQMSLRFQF"
     gene            1869..3992
                     /locus_tag="VFQ79_03845"
     CDS             1869..3992
                     /locus_tag="VFQ79_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEU0138812.1"
                     /translation="MWTRDRRIPAAIALFGALAVSLSGSPGDWPVYGYDAAATRYSPL
                     KQINTSNVARLRRAWTFHAGKPGSEAIPIVVGGVMYVTATNGIFALQPETGKQIWHFP
                     AEQVALRGLAYWPGDRKTHPRVFAGVRGGMIALDVTTGRPAAGFGNEGLLDLSQGVLD
                     PGLRGRVSLRSPPAVYGSTVITGSVNGEGTPSQGPYGDVRAWDAYTGKLLWTFHTVPR
                     PGEPGHETWPPGAWKSRSGTNAWGFLTLDLKRGIVYVPLGAPTSDFYGADRHGNNLYG
                     NSLVALDAATGKLKWYQQLVHHDLWDFDLAAPPILFDAALNGRVVPAVAEITKMGLLF
                     VFDRVTGEPIYGMEERPVPQSAVPGEKTAATQPFPLKPPPLSRITFTGSEIYDLTPEH
                     AAYCRDLFTKNRMKIGPLYAPLGLDGNTLTFPSTMGGGNWGGLSLDPTLGLLFTNVNN
                     LGQWGHMEGKTDPATGKFTYYRTSEHGPYARFWDRETRIPCSKPPFGELVAVDIRNGE
                     IAWRVPLGTVPALEALGVRNTGALNQGGPITTAGGLVFIAATNDARFRAFESRTGRLL
                     WEQSIDANGHTIPITYMGKDGKQYVALTAANGGAKFGGVPGDAVVAFALGPEEAPRPT
                     STASQPTVPAHTARPILLPEGSGKELLQRTCGTSCHKLDAVTGVRRTRDEWAAMVDYM
                     ISLGARANRDEVKLLVDYLAQHCGK"
     gene            4086..5195
                     /locus_tag="VFQ79_03850"
     CDS             4086..5195
                     /locus_tag="VFQ79_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138813.1"
                     /translation="MIVALACALLLAAASFAQEAPRREQAGPMDQLLLKDYKPEVSLV
                     VPQTRVPKAKYAAIDAHSHAYMRTPEQIAAWVRTMDEAGVETTVILTGATGANFDKLV
                     ELFLKPYPDRFQLYCGIDTSNPEAPDYPERAVAELVRCFRKGARGVGEVSDKGSGIAR
                     GQAVPPERRLHVDDPRLDPFWAKCGELKLPVNLHIADHPSAWRPPDQRQERTPNFQRY
                     NQWGRNVPGYEELLAKSERLLQKQPHTTFILCHLRNQGNDLAALAGILDRYPNLYVDI
                     SARHYEVGRQPRTAAKFLTRYSNRVLFGTDLSPTKEMYEGWWRLLETADEFIPGPNWW
                     RHYGLELPDSVLEPLYRGNAKRILNWQEVNIKASR"
BASE COUNT         1085 a         1572 c         1577 g          983 t
ORIGIN      
        1 gatgggcgcg acgtccggca tcaacggtca actggggctc cacggagtgg atcccgaagc
       61 gttcccgatc ataatcgtcc ccgggctctc caatctggga gcggggtcgg gccggtccac
      121 ctacccgatt gatacgtacc attttctcaa taacgtgacg tggattcgcg gctcgcattt
      181 catcaaaacg ggggttgagt ttcgctacgg cggccagacc aacaccaaca aggacctggc
      241 gggcggacag ttccggttcg gcgagcggat tacggggagc ggtctggccg aattactgtt
      301 ggggagggcg gaatccgccg agatacgctc tttcgaagac atttcggccc ggatcgacaa
      361 ctatggcgcg tttatccagg acgattggaa ggtgagccgg aatttgacgt tgaacctggg
      421 cctgcgctgg gagatggaca cgccgcggtg ggacaaagac aaccgcatga acagtttcga
      481 tctgaaagcg atcaaccccg tctgtaattg cccgggcgtg attacgtttg cgggactgga
      541 cggccgcagc aagtacgctc acgacttcga caagaacaac ttcggcccgc ggttcggctt
      601 tgcctggaac gccatgaacc gcatggttat ccgcggagga tacgccgttc attacaacgg
      661 ggcgtatcac agcgggattg ccggcgacat catcgcgggt ttcagtaacg aagcgtcctt
      721 cagttcgccg gacggcggca taaccccggc ggttcttttg cgtaacggca tgccggcgat
      781 tccggcaggc gaacccctga cgcccgcctt cggcgcagtt cccattggcg aaaacccgcg
      841 ctacgctcct gcgttcattc agcaaaacca cgtcaacgga tacgcgcacc agtggaattt
      901 cggcgttcag cgggagttgc ccgcgaacat gctcttcgag attgcctatc ttggcaaggc
      961 aggccataaa ctcggtggag acgaatacaa cctcaacgtc attccgctgg taaatggccg
     1021 cggaccggac gtgcagaggc aaaccgaccg tcccttcccg cagttcgaca acgtacaaca
     1081 taagttccca ccatggggca actccatcta tcacgcgatg aacgcgaagc tggagaagcg
     1141 gttctctcat ggcttgaact tcctgatgaa ttacactttt tcaaagttcc tggacgacgt
     1201 cgaaccaaag ggcgagttgg gcggcagcgg gctggacacc ggcattacgc atccggagtt
     1261 gaggcgtttc gacagagcgc cttccgggaa cgatatccgc caccgctaca ttgtctccgg
     1321 cgtttacgaa ttgccgttcg gcaagggccg gcggtgggga attgggaact ccgtgctcga
     1381 tcacattgct ggcggctggg gtctcggcgt aatcgccgag ttgcggtcgg gcgcacccat
     1441 cggagtgatc gagcaaacga accgcacaaa cacctactcc acaaacgtgc gtccaaacct
     1501 gctgcatgac cctggactca gccgggaccg cacccgcgcc gaaaaactcg cacagtggtt
     1561 tgacaccacc gcgtatgccg cgcccgccgt gggagagttc ggtaatgctc cacgcaccat
     1621 cgcggggctc ggaccgggtt atttcggact cgatgcgtcg gtgaacaaaa cctggagttt
     1681 gacggagcgt tacaggttga tgtaccgcgc cgatttctac aacgccccga actaccctgt
     1741 attcgctgct ccgaatcgtt cccgcgggag cgggaatttc ggacgaatca ccggcacaag
     1801 cggcaccggc cggcagattc agatgagtct tcggtttcag ttctgatatg gggcactctt
     1861 gtcatgaatt gtggacaaga gatcggcgga taccggcggc gattgcactg ttcggcgctt
     1921 tggcggtgtc cctttccggc tccccgggcg actggccggt ctacgggtac gatgcggcgg
     1981 caacccgcta ttcgcctctg aagcagatca atacgtcaaa cgttgcgcgt ctgcggcggg
     2041 cctggacatt ccatgccgga aaaccaggct ccgaggccat ccccatcgta gtcggcgggg
     2101 tgatgtacgt gacggccacc aacggcatct ttgcactcca gcccgagacc ggaaagcaaa
     2161 tctggcattt ccctgccgag caggttgcct tgcgcggcct ggcctactgg cccggcgaca
     2221 ggaagacgca tccgcgtgta ttcgctggtg tcaggggtgg gatgattgcg ctcgatgtga
     2281 ccaccggccg gccggcggcg ggtttcggca acgagggctt gctcgacttg agccagggag
     2341 ttctcgaccc gggcctccgg gggcgcgttt cactgcgctc gccgccggcg gtttacggca
     2401 gcaccgtgat taccggcagc gtgaacggag aagggacgcc ctctcagggt ccttacggag
     2461 acgtccgggc atgggacgcc tacaccggca aactgctctg gaccttccac acggtgccga
     2521 ggccgggcga gccgggtcat gaaacctggc ccccgggcgc ctggaagagc cgctccggca
     2581 ccaatgcctg gggctttctt acactggatc tcaagcgggg gatcgtgtat gtcccgctcg
     2641 gggctccgac ttcggacttt tacggtgccg accgccatgg aaataacctt tacggcaaca
     2701 gcctggtagc gctggacgcc gccacaggta agctcaagtg gtaccagcag ctcgttcatc
     2761 acgacttgtg ggatttcgac ctggcggccc cgccgatcct gttcgatgcc gctctcaatg
     2821 gccgcgtcgt accggcggtt gcggagatca ccaagatggg cctgctgttc gtcttcgacc
     2881 gcgtgaccgg agaaccgatt tacggcatgg aggagcggcc ggttccgcaa agcgccgtac
     2941 cgggggagaa gaccgccgcc actcagcctt tcccgctcaa gccgccaccg ctctcgcgta
     3001 tcaccttcac cgggagcgag atctacgacc tgacgcctga acacgccgcg tattgccgcg
     3061 atctgttcac gaaaaatcgc atgaagatcg gcccgttgta cgctccattg ggactcgatg
     3121 gcaatacact caccttcccc agcacgatgg gcggtggcaa ctggggcgga ctgtcgctcg
     3181 atcccacgct cggcctgttg ttcaccaacg tgaacaacct gggacagtgg ggccacatgg
     3241 aggggaaaac cgaccccgca accgggaaat tcacctatta ccgcacgtcc gaacacgggc
     3301 cctacgcccg tttctgggac cgggaaacgc gcattccatg ttcaaaacct cccttcggag
     3361 agctagtcgc ggtggacatt cggaacggag agattgcgtg gcgggttccg cttggcacgg
     3421 tccccgcgct cgaagcgctc ggcgtcagga acacgggcgc cttaaaccag ggtggtccga
     3481 tcaccactgc gggcgggctc gtcttcatcg cagcgaccaa cgatgcccgc ttccgcgcat
     3541 tcgagtcccg cacgggacgc ttgctctggg agcagtccat cgatgcaaac ggccacacca
     3601 tcccgatcac atacatgggc aaagacggca agcagtacgt cgcgctcacc gccgccaacg
     3661 gcggggcaaa attcggggga gtgccgggcg atgcagtggt agcttttgcg ctgggaccag
     3721 aggaggcgcc gcgccccacg tccacggctt cgcagcctac agtacccgcc cacaccgcgc
     3781 gtccgattct gttgccggaa ggttcgggta aggaactact ccagcggacc tgcggaacca
     3841 gttgccataa actcgacgcc gttaccggcg tgcgccgcac gcgtgacgag tgggccgcca
     3901 tggtcgatta catgatctcg ctcggagcgc gcgcgaatcg ggacgaagtg aagctattgg
     3961 tcgattacct ggcgcagcac tgcgggaaat aggatctgat catccttgac cggcagacca
     4021 aatcagcata ttatcgataa cggtagcgag ccctaacgga acgactgatg agatttgaga
     4081 gaacaatgat cgttgcactg gcatgcgcgc tgctgctagc agcggcatct tttgctcagg
     4141 aggcaccccg gagggagcag gccgggccga tggaccagtt attgctgaag gactacaagc
     4201 ccgaggtctc gctggtcgtg ccccaaacac gcgtgccgaa agcgaaatac gccgcgattg
     4261 atgctcactc tcatgcctac atgcgcacgc cggagcagat cgctgcatgg gtgcgcacga
     4321 tggacgaagc gggggtggag acgacggtca tcctgaccgg cgctacgggt gccaatttcg
     4381 acaaactcgt cgagctgttc ttgaaaccgt atcccgatcg tttccagctt tattgcggca
     4441 tcgacaccag caaccccgag gcgccggact atcccgaacg cgccgttgcc gaactggtcc
     4501 gctgcttcag aaaaggcgca cgcggagtag gcgaggtctc agataaagga tccggaatag
     4561 ctcggggaca ggctgtacct ccagagcgga ggctgcatgt cgacgatccg cggctggatc
     4621 cattttgggc gaagtgtgga gaactcaagc ttcccgtgaa cttgcacatc gcggatcatc
     4681 catccgcgtg gcggccgcca gatcaacggc aggaacggac gccaaacttc caacgctaca
     4741 accaatgggg ccgcaacgtt ccgggttacg aagaactact tgctaagagc gagcggctcc
     4801 tgcaaaagca accgcacacg actttcattc tctgtcacct cagaaaccag gggaatgatc
     4861 tcgccgcatt ggccggcatt ctcgaccggt atccaaatct ctatgtcgac atatctgctc
     4921 gtcattacga agtcggacgg cagcccagga ctgccgctaa gtttctgacg agatactcga
     4981 atcgggtatt gttcggcacc gatctatctc cgaccaagga gatgtacgaa gggtggtggc
     5041 gtctgctcga gactgccgac gagtttatcc ctggaccgaa ttggtggcgt cattacgggc
     5101 tcgaattacc ggattcggtg ctcgagcctc tatatcgcgg aaacgccaaa cgcatcttaa
     5161 attggcagga agtgaacatc aaagcgtcgc gttgacaaag aaaggagacg caccgtg
//
LOCUS       DASTMK010000096         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_37063, whole genome shotgun sequence.
ACCESSION   DASTMK010000096 DASTMK010000000
VERSION     DASTMK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37063"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFQ79_03855"
     CDS             <1..447
                     /locus_tag="VFQ79_03855"
                     /inference="COORDINATES: protein motif:HMM:NF045331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138814.1"
                     /translation="VEAVKKLESEVRTGKIPEEPDPVARSKKIFLLCLGVAYSRFKDA
                     LEQEQEVLAGLSDLAMQAYAMESATLRAARTKSAGEMARVFVQDGMAQMDNTARDILA
                     ACLEGEPLRATAGVLHRFAKVELVNAVELRRRIASRLLEAGKYVVD"
     gene            449..931
                     /locus_tag="VFQ79_03860"
     CDS             449..931
                     /locus_tag="VFQ79_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0138815.1"
                     /translation="MHPAAAKPRIVVLVGLPGSGKSSYARERNLPVLSSDHIRELLVD
                     DATNQSIHGLVFATLRSLLRRRIELGQPETYIDATNLTRKERRAYIKMGEFYGCVVDA
                     IFFDVPVEVCRERNRARSRVVPDEALLAMAAKLRPPEASEGFSNILVVRDRPRPLPEP
                     "
     gene            complement(881..2185)
                     /locus_tag="VFQ79_03865"
     CDS             complement(881..2185)
                     /locus_tag="VFQ79_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0138816.1"
                     /translation="MTANPVSLSAYGRLIRTNRNFRLLWLAQIISELGDWLYSVAVYS
                     LLFEYTGSAKSVATAFVLQVLPQFFIAPAAGVINDRLSRKKVMIFADCFRAVIVLGML
                     LVRGPEMIWFLYILLLLETCMWALFEPGRNAVIPNITTGKEVIVANALSSMTWSFNLA
                     IGAALGGAIAALFGRTTVFLVNGLTFVVSALVLRRMEFSEPHLAMLPPLRMRDLADFT
                     PILDGIRYVKHDARLLATMFVKCGLGLMGSHWVILPVLGERVFPIHMEGLDPQRGGML
                     GMSLLMASRGVGALLGPIVGGYWSGRVESRLRLGILLGFLTGSLGYLLLSAAGSLWTA
                     CATVVLAYAGGSIIWVFSTTLLQLQTADRFRGRVFSAEFAFGMLAMSTVSYTSGVLID
                     VGLTPQELAAYTGLIVLIPAAAWAFALRLWKRPRPVPNHQDI"
     gene            complement(2186..3175)
                     /gene="trpS"
                     /locus_tag="VFQ79_03870"
     CDS             complement(2186..3175)
                     /gene="trpS"
                     /locus_tag="VFQ79_03870"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU0138817.1"
                     /translation="MKARVFSGVQPSGDLHIGNYLGALKNWVRIQYDYESIFCIVDLH
                     AITVYQEPAALRAKIEEIAAIFLASGIDPKHCAIMVQSSVPAHAELAWLLTCVTPVGW
                     LERMTQYKMKAESQETVGDGLLQYPVLMAADILLYQAAIVPVGEDQRQHLELTRDVAQ
                     RFNSLYGDTFVMPETHLPTVGARVMGLDDPEKKMSKSASGSGHAVALLDPPAQAKKKI
                     MRATTDSLPAVDFENLGAGVENLLGIFQAFSEWPEDRMKKHFAGMRYGDLKKQVSEMV
                     ISHLEPFQKRYREITSEAGYVASILREGAARVAPIADATVELVKKRMGLYTLS"
     gene            3207..4415
                     /locus_tag="VFQ79_03875"
     CDS             3207..4415
                     /locus_tag="VFQ79_03875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU0138818.1"
                     /translation="MAASKTNNIEFEKWVYGGDALGRADGRVVLAPFVLPGETAEVRV
                     TEEKPGLVRAFPVSISIPSPERVEAPCPYFGRCGGCHYQHAPYEYQVKQKREILLEAL
                     RRIGKIEAAPGIGVVTGEPWQYRNRAQFHIAVGEVGYLQMRSHRLCPVSHCPISSPKI
                     NEALAKIREMMRERRWPKFVRTLELFTNEESVQVNVPETSQPVARSFFEWCQERIPGA
                     THQAIEYRAAGEVYRVGYRSFFQVNRFLIDRLMDSALQGAEGAHALDLFAGVGLFSLP
                     LARRFGRVTAVESGASAVKDLEFNARNAGVTVAIQRAVVEEFLSGYAEPPDFILADPP
                     RAGLGKHVVRHLLRLRAPRLTIVACDPATLARDLSALIEGGYRIERMEMVDLFPQTFH
                     IETVAALTLR"
     gene            4412..>4734
                     /locus_tag="VFQ79_03880"
     CDS             4412..>4734
                     /locus_tag="VFQ79_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138819.1"
                     /translation="MKTTIVLSTPAGIALLLALSAAAFAAGHHPVFRVDTSNPTVDKP
                     QSKTWYAEGSWWALLPRKSGPSLWQRTPSGWHERREITRSLSGLPGRADVWFDKDGAT
                     AVVVK"
BASE COUNT          988 a         1372 c         1493 g          881 t
ORIGIN      
        1 gtggaagcag tgaagaagtt ggagagcgag gtccgcacgg ggaagatccc ggaggagccg
       61 gacccggtcg cacgatcgaa aaagattttc ctgctgtgcc tgggggtggc ctatagccgt
      121 tttaaagatg cgctggagca ggaacaggaa gtgctggcgg gcctgtcgga tctcgcgatg
      181 caggcctacg cgatggaatc cgcaacgctg cgggcggcca ggacgaagtc cgccggtgag
      241 atggctcgcg tatttgttca ggacggcatg gcgcaaatgg ataacaccgc gcgcgacata
      301 ctggcggcgt gtctcgaagg agagcctctt cgagccacag ccggagtgct ccaccgcttt
      361 gccaaggtcg aacttgtgaa cgcggtcgag ctgcggcggc gcatcgcttc caggctgctc
      421 gaagccggaa agtacgtggt cgattagcgt gcatccggcg gccgcgaaac ctcgcatcgt
      481 tgtgctggtg ggactgcccg ggtccggcaa atcatcatac gctagggaac gcaacctgcc
      541 ggtgctttcc tccgatcata ttcgggaact gcttgtggac gacgcgacga accagtccat
      601 tcacgggctg gtgttcgcca cgttgagaag tttgctgcgc cggcgaatcg agttggggca
      661 gcccgaaacc tatattgacg caacgaacct tacacgcaag gaacggcggg catacatcaa
      721 gatgggcgaa ttctacggct gcgtggtgga cgccattttc ttcgatgtgc cggtggaggt
      781 gtgccgggaa cgaaaccggg cgcggtcgcg cgtggtgccg gatgaagcgc tgcttgcgat
      841 ggccgcgaag ttgagaccgc cggaagcgag cgagggcttc tcaaatatcc tggtggttcg
      901 ggaccggccg cggccgcttc cagagccgta gcgcgaatgc ccaggcagca gcgggaatga
      961 ggacgatcaa acccgtgtag gcggccagct cctgcggtgt caatccgaca tcgataagca
     1021 ctccggacgt atagctgacc gtggacatgg cgagcatacc gaaggcgaat tccgcggaaa
     1081 agacgcgacc gcgaaagcgg tccgcagtct ggagctggag tagcgtggtg gagaaaaccc
     1141 atataatgga gccgccggcg taagcgagca ccacggtagc gcacgcagtc cacagagaac
     1201 cggcggcgga gagcagcagg tagccgagcg aaccggtgag gaagccaaga aggataccga
     1261 gccgcagccg ggattcaacg cggcccgacc agtagccacc cacgatgggg ccgaggagcg
     1321 ctcctactcc acgcgatgcc atcagcaggc tcatgcccag cattcctccg cgctgcgggt
     1381 caagaccctc catgtggatg ggaaagacgc gctctcccaa aaccggcagg atcacccaat
     1441 gcgatcccat cagtcccagc ccgcacttga cgaacattgt cgccagcaga cgcgcatcgt
     1501 gcttcacgta acgtatgcca tccagaatgg gcgtgaaatc ggccaggtcg cgcattcgta
     1561 gcggcgggag catcgcaagg tgaggttccg aaaattccat ccggcgaagc accagggcgg
     1621 acacaacgaa cgtgagtccg ttcacgagaa ataccgtcgt ccggccgaac agcgccgcga
     1681 tagcaccgcc cagcgcggct cctatggcga gattgaacga ccaggtcatg gaggacagcg
     1741 cgttcgccac aattacttct ttgccggttg tgatgttcgg aatgactgca tttcgtccgg
     1801 gctcgaacaa agcccacatg caggtttcga gaagaagcag gatgtacagg aaccagatca
     1861 tctccggccc gcgcaccagc aacattccca atacgatgac cgcacggaag cagtcggcga
     1921 agatcatgac ttttttgcgg ctgagccggt cattgatcac accggcggcg ggcgcgatga
     1981 aaaattgcgg cagaacctga aggacaaaag cggtcgccac ggacttggcg cttccggtgt
     2041 actcgaacag caggctgtac accgccaccg agtagagcca atctcccagt tcgctgataa
     2101 tctgtgccag ccacaggagc ctgaaattcc ggttcgtgcg aatcaggcgt ccgtaggctg
     2161 aaagcgaaac cgggttcgcg gtcatctagg agagggtata caggcccatt ctcttcttta
     2221 cgagttcgac ggtagcgtcg gcgatcgggg ctactcgcgc cgcgccttcg cgaagaatgg
     2281 aggcgacata tccggcctcc gacgtgatct cgcggtaccg cttttgaaac ggctccaaat
     2341 gggagataac catttccgac acctgcttct tcaaatctcc gtaacgcatc ccggcgaagt
     2401 gcttcttcat ccggtcctcc ggccattcgc tgaacgcctg aaagatgccg aggaggttct
     2461 cgacgccggc gccgagattc tcgaaatcga ccgcgggcag cgaatcggtt gttgcgcgca
     2521 tgattttctt tttggcctgc gccggcggat ccagaagcgc cacggcatgc ccggagccgg
     2581 aagcggactt gctcattttc ttctcggggt cgtccaggcc catcacacgc gcacccacgg
     2641 taggaaggtg cgtttcgggc atcacgaacg tgtcgccgta aagcgagttg aaccgctgtg
     2701 ccacgtcgcg tgtgagttcc aggtgctgcc gctgatcctc tcccacgggc acaatggcag
     2761 cctggtacag aaggatgtcg gcggccatca ataccggata ttgcagcagc ccgtcgccca
     2821 cggtctcctg cgattcggcc ttcatcttat actgtgtcat gcgctccagc cagcccaccg
     2881 gagtaacgca ggtcagcagc caggccagct cggcgtgcgc cggcacggag gactgcacca
     2941 taatggcgca gtgctttgga tcaatgcccg aggccaggaa aatcgccgcg atctcttcga
     3001 ttttggcgcg cagcgcggca ggctcctggt atacggtgat ggcgtgaagg tctacgatgc
     3061 agaaaatgct ctcgtaatcg tactggatcc ggacccagtt cttgagagcg ccaaggtaat
     3121 ttccaatgtg aaggtcaccg gatggttgga cccccgagaa aactcgtgct ttcatgctaa
     3181 taagagacga agttttatcc tacgtgatgg cggcctccaa aacaaataac atcgaatttg
     3241 aaaagtgggt ctacggcggg gacgcgcttg ggagggccga cggccgtgtc gtgcttgcgc
     3301 cgtttgtcct gccgggcgag accgccgaag tccgggtgac cgaggaaaaa ccaggtctcg
     3361 tgcgggcgtt tcccgtcagc atcagcatac cgagcccgga gcgcgtggag gctccgtgcc
     3421 cctacttcgg ccgctgcggc gggtgccatt accagcatgc cccctacgaa tatcaggtga
     3481 aacagaaaag ggagattctg ctcgaagcct tgcggcgtat cgggaagatc gaagcggcgc
     3541 ccgggatcgg tgtggtaacg ggggagccgt ggcaataccg caatcgcgcc cagttccaca
     3601 tcgcggtggg cgaagttgga tatctgcaaa tgcgctccca ccggctgtgt ccggtgtcgc
     3661 actgcccgat ctcttcgccg aagatcaatg aggccctggc caaaattcgc gaaatgatgc
     3721 gggagcgccg ctggccgaaa ttcgttcgca ccctggaact ctttacgaac gaggagagcg
     3781 ttcaggtaaa cgttccggag acttcgcaac ccgtcgcacg atcgttcttc gaatggtgcc
     3841 aggagcgcat tccgggagcg acgcaccagg ccatcgaata ccgcgcggca ggcgaagtat
     3901 accgtgttgg ctaccggtcg ttctttcagg tgaaccgctt tctgattgac cggctgatgg
     3961 actcggcgct tcagggagcg gagggcgcgc acgcgctgga cctgtttgcg ggcgtcggac
     4021 ttttcagtct gcccttggcg cgacgtttcg gaagggttac agcagtggag tctggtgcaa
     4081 gcgcggtgaa agacctcgag ttcaacgcac ggaatgccgg cgtaaccgtc gccatccaac
     4141 gggcggtagt ggaagaattt ctgtccggct acgcggagcc gccggatttc attctggccg
     4201 atccaccgcg cgccggtttg ggaaaacacg tggtgcggca tctgctgcga ttgcgcgcgc
     4261 cgcggctgac gattgtcgct tgcgatccgg cgacgctggc tcgcgacctg tcagcgctca
     4321 tcgagggtgg ttaccggata gagcgaatgg agatggttga tctgtttcca caaacctttc
     4381 acatcgagac agtggccgcg cttaccctgc gatgaaaaca acaatcgttc tctccactcc
     4441 tgcgggcatc gctctgttac tcgcactatc cgcggctgcc ttcgccgcgg gccaccaccc
     4501 ggtgtttcga gtggatacct ccaatccaac cgtggacaag ccgcaatcga agacctggta
     4561 tgcggaagga tcctggtggg cgctgcttcc gcgcaagtcc ggtccctcgt tgtggcagcg
     4621 gacgccatcc ggatggcacg agcgtcggga gattacccgg agtctgagcg ggcttcccgg
     4681 acgcgccgac gtctggtttg ataaggacgg cgcgacagcc gttgttgtaa agga
//
LOCUS       DASTMK010000097         5790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_196939, whole genome shotgun sequence.
ACCESSION   DASTMK010000097 DASTMK010000000
VERSION     DASTMK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196939"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1457)
                     /gene="gltA"
                     /locus_tag="VFQ79_03885"
     CDS             complement(21..1457)
                     /gene="gltA"
                     /locus_tag="VFQ79_03885"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEU0138820.1"
                     /translation="MGETRPKRNPKAPRQELPVLDPKLRASTPQEVALGFTTEQAGIE
                     ALRCLNCKDAPCVTACPLHIDIKSFIYRMVSGDCHGAFEKISEQSPFPGVCGRVCQHE
                     LFCEDACLLGRKLDPVAIGSLERFAADYNRQLGSDGHGKLPKPTGPRVALVGSGPASM
                     IAAFDLVRKNYRVTIFEALHELGGVLAYGIPPFRLPREILNAEIERLKEMGVEFETNF
                     IVGRTATVQELFDEGFEAIFIGTGAGLPWLLNIPGENLIGVYTANEFLTRINLMRAYK
                     EGAETPVRVGKHTVVIGGGNSAMDAARWAKRLGSETTILFRRGRKELRARLEEIEHAE
                     EEGIHFSFLAAPVRLFGDERGCVREMECIRMELGEPDSSGRPAPVPVPGSEYRIPVDT
                     VVAAIGQAPNPTLQRATPQLITNRGKIVVTETGQTNLPMVFAGGDVVRGGSTVILAMR
                     DGRVAAEAIHQALSSKQEPADTTLEVRQ"
     gene            1661..2965
                     /locus_tag="VFQ79_03890"
     CDS             1661..2965
                     /locus_tag="VFQ79_03890"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEU0138821.1"
                     /translation="MRTVDVIQRKRDGEELSPEEITYLVDGYTRGEIPDYQMAAFLMA
                     VFHSGMTDREVSALTETMITSGETVDLSDIPGIKVDKHSTGGVGDKTSLIAAPLAASA
                     GAVVPMISGRALGHTGGTLDKLESIPGFRTNLTIDEFREQLHRTGLCFIGQTPQVAPA
                     DGRFYALRDATATVESIPLIASSIMSKKIAMGIDALVLDVKVGSGAFMKRQVDARRLA
                     QMMVGIGRRVDKRVQALITDMNQPLGFAVGNALEVMEVSQTLQNAGPTDLTRLSLELA
                     ARMIHLGKQAQTIEEAREIAQSKLLDGSGYRKFKEVIEAQGGNPQVLDRFELLPNATG
                     VREITSPRAGYVTAIDAGDIGRASAMIGAGRDTKEDTIDPAVGVILEVKTGQKIDAGS
                     VLCRLYHTREDDLDEGAQLVEDAFRISTTMPEERALILEVVS"
     gene            2979..4202
                     /locus_tag="VFQ79_03895"
     CDS             2979..4202
                     /locus_tag="VFQ79_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEU0138822.1"
                     /translation="MGRFTGLLGIAIILATAYLFSTNRRAIRPQLLLWGLGLQFVVAF
                     LVLRTGFGWLFQAASTGVNALLEFAETGSQFVFGPLGAKSGPFGVVFAFQVLPIVIFI
                     ASFFSILYYFGVMQIFVRAMAVVMQRVMGASGAESTNVAASIFMGQTEAPLTVRPFLA
                     GMTQSELFTIMTSGMAHVSGAVMAAYVKIAGVDIKHLLTAVIMTAPATILLAKMLMPE
                     TEKPETAGNVKIDVERPGVNVIDAAARGAGEGLHLALNIGAMLIAFLALIAMANGILG
                     WVHSLPWMGWLPPTLQQIFGYIFSPVAWVLGVSWKDSMAIGNLLGTRLVLNEFVAFLE
                     LAPLREQLDPRSFVIATYALCGFANFSSIAIQIGGIGALAPSRKSDLARLGLKAVAAG
                     TMANFMSACIAGMLL"
     gene            4199..5023
                     /locus_tag="VFQ79_03900"
     CDS             4199..5023
                     /locus_tag="VFQ79_03900"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU0138823.1"
                     /translation="MSIAGALQFLESRIQIRPRVAVVLGSGLGGFADELTDAIAIPYD
                     EIPGWPRSTAVGHAGQLVFGKLGAVDVAVMSGRVHLYEGYTQYQVTLGVRVLGRLGVR
                     SLVLTNAAGGIHPEYRRGALVLLNDHINLQGSNPLVGPNDDSLGPRFPDMTEAYSAEF
                     RELARQVGDEQGIPLKEGVYAALLGPSYETPAEIRFLRTIGADLVGMSTVPEVIVANH
                     TRMRVLAISCVTNMAAGILPQKISHEEVLETGREIRETLIRFLKALLARVGERWTD"
     gene            5011..5397
                     /gene="cdd"
                     /locus_tag="VFQ79_03905"
     CDS             5011..5397
                     /gene="cdd"
                     /locus_tag="VFQ79_03905"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU0138824.1"
                     /translation="MDGLTAAALAARENALAPFSGFRVGAAVEDDSGRIHTGCNIENA
                     SFGLTICAERVAVFKALSEGSRAFRRLAVAADTDRLTPPCGACRQILWEFCGNIEVVL
                     VNLAGSTESHRMRDLLPEPFDASLLR"
     gene            5378..>5790
                     /locus_tag="VFQ79_03910"
     CDS             5378..>5790
                     /locus_tag="VFQ79_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138825.1"
                     /translation="MLRYCVSLLVAVTVFASEPADWVITARYVVTMNPGREVIEDGAV
                     AVNGAAIAAVGKRAEIERRFDARKRLDRPDALIAPGLINTHTHAAMSLFRGIADDLRL
                     QDWLEKYIFPAEAKNVSSEFVRWGTKLACLEMMLSG"
BASE COUNT         1135 a         1720 c         1730 g         1205 t
ORIGIN      
        1 acgcctggta acaattctgt tcactgccga acctccaggg tggtgtccgc cggctcctgc
       61 ttcgaactga gagcctgatg gatggcctcc gccgccaccc gtccatcacg catcgccaga
      121 atcactgtcg atccaccgcg aaccacatcg cctccggcaa acaccatcgg caggttggtc
      181 tgtccggttt ctgtgacgac gatctttccg cggttcgtga tcaactgcgg cgtggcccgc
      241 tgcaaggtcg gattaggcgc ctgtccgatc gcagcaacca ccgtgtccac cggaatgcga
      301 tattctgagc ccggcaccgg tacgggcgcg ggccgcccgg acgaatccgg ctcgcctaac
      361 tccatccgaa tgcactccat ctcgcgaacg catccgcgct catcgccgaa gagacggacg
      421 ggggcggcga gaaaggagaa gtgaatgcct tcctcctcag catgctcaat ctcttccagg
      481 cgcgcccgca gttccttgcg gccacggcgg aacaggatag tggtctcgct acccagccgc
      541 ttggcccacc gcgcggcgtc catcgcggaa ttgccacctc caataacaac ggtatgcttg
      601 ccaacccgta cgggcgtttc ggcaccctcc ttataagcac gcatgagatt gatacgcgtc
      661 agaaactcgt tggccgtgta cacgccaatc agattttcgc cggggatatt cagcaaccac
      721 ggaagaccgg cgcccgttcc gataaagatc gcctcgaatc cctcgtcaaa cagctcctgc
      781 acggtggcgg tccggccgac gataaaattc gtttcgaact cgacgcccat ctccttcaga
      841 cgttcgattt ccgcattcag gatttcacga ggcaggcgga aaggcggaat tccgtaggcc
      901 aggacaccgc ccagctcgtg cagagcctcg aaaatcgtaa cgcggtaatt cttgcggacc
      961 aggtcgaacg ccgcaatcat cgaggcgggt ccgctaccta ccagcgcgac acgaggaccg
     1021 gttggtttag ggagcttccc atgtccatcc gatccgagct gccgattata gtcggccgcg
     1081 aagcgttcca gcgatccgat tgcgaccgga tccagcttcc ggcccagcag gcatgcgtct
     1141 tcacagaaca gttcatgctg gcatacgcgc ccgcatacgc cgggaaacgg gctttgctcc
     1201 gagatttttt cgaatgcgcc atggcaatcg cccgatacca tccggtaaat gaaactcttg
     1261 atatcgatat gcagcggaca ggcagtcaca caaggcgcgt ctttacagtt caggcaacga
     1321 agagcttcaa tcccagcctg ttccgttgtg aagccaagcg ctacttcctg cggcgtcgat
     1381 gcacgcaact taggatcgag tactggtagc tcctgacggg gcgccttcgg gttgcgtttt
     1441 gggcgggttt cccccatttg tgcgtcaaat ccccctattt tcaagcaaaa tacttctaat
     1501 ccagtcagac gtgcagttcg tattccattg gtgcgcaccc cgcgtctaaa gattttcgaa
     1561 tcttcaacac ttttgctccg gctcaggaga aacgcagaac aaaatcgcgt actgttcctt
     1621 tactttcaaa catatatggt gttacgctaa atttctcata atgcgtacag tcgacgtgat
     1681 ccagcggaag agagatggcg aagagctgag ccccgaggaa atcacctacc tcgtggacgg
     1741 ctatacgcgc ggggagatcc ccgactatca gatggctgcg tttctgatgg ccgtcttcca
     1801 ctccggcatg accgaccgcg aagtcagtgc cttaaccgaa acaatgatta cgtccggtga
     1861 gactgtggac ctgtcggata taccggggat caaagtcgac aaacattcga ccggaggtgt
     1921 cggcgacaaa acgagtttga tcgcggcgcc gcttgctgcc tcggccggcg ctgtcgtccc
     1981 gatgatttct ggcagggccc tggggcacac cggcggtacg ctggacaaac tcgaatcgat
     2041 accggggttc cggacgaatc tcaccatcga cgaatttcgc gaacagttgc atcgcaccgg
     2101 cctttgcttt atcggccaga caccgcaggt cgctccggca gacgggaggt tctacgcgct
     2161 gcgggatgca accgcgacgg tcgaatccat cccgctcatt gcctcgtcga tcatgtcgaa
     2221 gaagatcgcg atgggcatcg acgcgcttgt gctggatgtg aaagtcggct ccggcgcgtt
     2281 tatgaaaagg caggtggatg ccagacggct ggcacagatg atggtaggta ttggccgccg
     2341 ggtggacaaa cgggtacagg cgctgatcac cgatatgaat cagccgcttg gtttcgccgt
     2401 gggaaacgcc ctggaagtga tggaagtttc ccagactttg caaaacgccg gtcccaccga
     2461 tctcacgcgg ctatctctcg aattagcggc ccggatgatt catctcggca agcaggcgca
     2521 aaccatcgag gaagcgcgcg agattgcgca gagcaagctg ctggatggtt ccggttaccg
     2581 caagtttaaa gaggtgatcg aggcgcaggg cggcaatccg caggtgctcg accggtttga
     2641 gttgttgccg aatgccaccg gagtacgcga gatcacgtct ccacgcgcgg gctatgtcac
     2701 cgccattgat gccggcgata tcgggcgcgc ttccgccatg attggagccg gccgcgatac
     2761 gaaagaggac accatcgacc cggctgtcgg tgtcatcctc gaagtgaaaa caggccagaa
     2821 gattgacgcc ggctcggtgc tttgccggtt gtatcacacc cgcgaagacg acctggacga
     2881 gggcgcacag cttgtggagg acgctttccg catctctaca accatgccgg aagagcgcgc
     2941 actcatcctg gaagtcgtca gttgaaatag cgctcccgat gggccgcttc acagggctgc
     3001 tcggaatcgc catcattctg gctacggcgt atctgttctc gacgaaccgc cgcgccattc
     3061 gcccccaact tcttctgtgg ggcctcggcc tccagtttgt tgtcgccttc ctggtattgc
     3121 ggaccggctt cggctggttg tttcaggctg ccagcaccgg agtgaacgct ttgctcgaat
     3181 ttgcggagac aggcagccag ttcgtatttg gtccgcttgg cgcgaaatcc ggtcccttcg
     3241 gagtcgtctt cgctttccag gtgctgccca tcgtcatctt catcgcttcg tttttctcga
     3301 tcctgtacta cttcggcgtc atgcagattt tcgtgcgcgc catggccgtc gtgatgcagc
     3361 gcgtcatggg cgccagcgga gctgaatcca ccaatgtcgc cgccagcatc ttcatggggc
     3421 agaccgaagc tcctcttacc gtgcgtccgt tcctggccgg tatgacacag tccgagctat
     3481 tcacgatcat gaccagcggg atggcacatg tctcgggagc ggtgatggcc gcatatgtga
     3541 aaatcgccgg cgtcgatatc aagcacctgc tcaccgctgt gattatgacc gcacccgcaa
     3601 ccattctgct cgccaagatg ctgatgccgg aaacggaaaa accggagacc gcgggcaacg
     3661 tgaaaatcga tgtggaacgg cccggcgtga atgtgatcga tgcggccgcg cgtggagccg
     3721 gcgagggact tcacctggcg ttgaacatcg gtgcaatgct cattgcgttc ctcgcgctga
     3781 tcgccatggc gaacggaatt ctcggctggg ttcacagcct tccctggatg ggctggctgc
     3841 cgccgacgct tcagcagatc ttcggataca tcttctctcc cgttgcctgg gtgcttgggg
     3901 taagttggaa agattcgatg gcgatcggca atctcctggg cacgagactg gtgctgaatg
     3961 aatttgtcgc gttcctggaa ctcgcaccgc tgagggagca actcgatccg cgatcgtttg
     4021 tcattgcgac ctatgccttg tgcgggttcg caaacttcag ctccattgcc atccagatcg
     4081 gcggtatcgg ggcactggcg cccagcagga aatcggatct ggctcggctc ggattgaaag
     4141 ccgtcgcggc gggtaccatg gcgaatttta tgtccgcctg tatcgcgggg atgctgttgt
     4201 gagcatagcc ggagcgcttc agttcctgga atcccgcatc cagatccgcc cgcgcgtagc
     4261 ggtggtgctc ggcagcggcc tgggtggatt tgcggacgag ctcacggatg ccattgcgat
     4321 tccgtacgac gagattcccg gctggccgcg gtcgacagcg gtaggacacg cgggtcaact
     4381 ggtgttcggc aagctgggcg cggtcgacgt tgccgtgatg tccggacgcg tgcatctgta
     4441 cgaaggctac acacagtatc aggtgacgct tggtgtgcgg gtgctgggac gcctgggggt
     4501 gcgctctctt gttttaacca acgccgcggg aggcattcat cccgaatacc ggcgcggcgc
     4561 cctggtgttg ctgaacgatc atatcaacct gcaaggttcg aacccgctgg tgggacccaa
     4621 cgacgattct ctcggcccgc gttttcccga tatgaccgaa gcctactccg ccgaatttcg
     4681 ggagttggcg cggcaagtgg gcgacgaaca gggcatacct ctcaaggagg gagtctatgc
     4741 cgccctcctg ggccccagtt acgagacacc cgcggagatc cgcttcctgc gcaccatcgg
     4801 agcggacttg gtaggcatgt ccaccgtacc cgaagtcatc gtggcgaatc acacgcgcat
     4861 gcgggtgctg gcgatttcct gtgtgacaaa tatggccgcg ggcatcctgc cgcagaagat
     4921 ctcgcatgag gaggtgctcg aaaccggccg cgagattcgc gagacgttga tccgttttct
     4981 aaaggcgcta cttgcacgcg taggagagcg atggacggac tgacggcagc ggcgcttgca
     5041 gcccgcgaga acgcgctcgc gccattctca ggctttcgtg tcggcgcggc cgtggaggac
     5101 gacagcggcc gcattcacac cggctgcaat atcgagaacg caagcttcgg cctgacaatc
     5161 tgtgccgaac gcgtggccgt ttttaaagcg ttgtcggaag gatcgcgagc ctttcgccgc
     5221 ctggcggtag ccgcggatac agatcgactg acaccgccat gcggagcctg ccgccagatt
     5281 ctatgggagt tttgcgggaa cattgaggtg gtgctggtga atctcgccgg cagcacggaa
     5341 tctcaccgga tgagagacct tctgccggag cccttcgatg cttcgttatt gcgttagcct
     5401 cctcgttgcc gtcacggtct tcgcgtccga gcccgccgat tgggtgatca cggcgcggta
     5461 cgtagtcacg atgaatccgg gccgggaggt gattgaagac ggcgctgtgg cggttaacgg
     5521 agcggcgatt gccgccgtgg ggaagcgcgc cgaaatcgag cggcgctttg atgcgcggaa
     5581 gagactggac cgtccggacg cgctcatcgc tcccggcctc atcaacacgc acacgcatgc
     5641 ggcgatgtcg ctgtttcgcg gcatcgcgga cgacctgcgg cttcaggact ggctcgagaa
     5701 atacatcttc ccggccgaag cgaaaaatgt ctcgtccgag tttgtacggt ggggcacgaa
     5761 actcgcctgt ctcgaaatga tgctttccgg
//
LOCUS       DASTMK010000098         6435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_196977, whole genome shotgun sequence.
ACCESSION   DASTMK010000098 DASTMK010000000
VERSION     DASTMK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196977"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VFQ79_03915"
     CDS             <1..1022
                     /locus_tag="VFQ79_03915"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEU0138826.1"
                     /translation="EELAAAVHGVADELGRPAYVIAESDLNDNRLVRLPADGGYGLDA
                     QWNDDFHHSLHTLLTKETSGYYADFGGLDHLERAIAEGFVYAGQYSCYRGRCHGTSSV
                     DLAADRFIVFSQNHDQIGNRMMGTRLANMISFESAKLAAGVVLLSPFIPLLFMGQEYA
                     ETAPFLYFVSHTDPDLVRAVREGRKDEFAAFAWQGELPDPQAEESFLQSKLNPSLRNT
                     GRHRVMWEFYRNLIELRKSTTALAVLDKNSLELSRFDDSLSLAMRRWAQQDQALVVFG
                     FSESEQTIQVALPPGTWSKALDSAGTEWMGTGSTAPEHLESNGAATFRLRGESFVVYR
                     LKASG"
     gene            complement(998..2506)
                     /locus_tag="VFQ79_03920"
     CDS             complement(998..2506)
                     /locus_tag="VFQ79_03920"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU0138827.1"
                     /translation="MELTRRGLLKAGAFGILSRRGVAAAKRPNVLFLMADQHRGDCLG
                     ADGNRAVRTPNLDRIAAGGVRFRCAYSSTPTCTPARAGLLTGMSPWNHGMLGYGRVAE
                     KYPVEMPRAMREAGYYTMGIGKMHWHPQRNSHGFHQVLLDESGRAESPDFRSDYRAWF
                     WSEAPGLDPDATGIGWNDYPAKAYVLPERLHPTRWTGDCAVRFLEGYRRPEPFFLKVS
                     FARPHSPYDPPQRFMDLYADADLPEAHVAPWAGRYRERNSDRYDIWRGNLRPAQVRSS
                     RQGYYGSVSFIDEQIGRILDALEKRSMLEETLILYTSDHGDMTGDHNLWRKSYAYEPS
                     ARVPMLMRWPAGMLSAKRGQVMANPVELRDLLPTFLDAAGSSPARPLDGASLLSLVKN
                     GGKEWRSEIDLEHDVCYSPENHWSALTDGRMKYIFHARDGEEQLFNLESDRHEMKNLA
                     GDVGSSGELKTWRDKMINYLAVRGDPWVVNGKLGLRPERQTYSPNYPEAFNR"
     gene            complement(2570..2785)
                     /gene="iscX"
                     /locus_tag="VFQ79_03925"
     CDS             complement(2570..2785)
                     /gene="iscX"
                     /locus_tag="VFQ79_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEU0138828.1"
                     /translation="MAKLTWDDTENIALALYDKYPDLDPLTVRFTDMHRWITELSGFS
                     DDPALSTEGKLEGIQMAWYEEWKDNQE"
     gene            complement(2785..4638)
                     /gene="hscA"
                     /locus_tag="VFQ79_03930"
     CDS             complement(2785..4638)
                     /gene="hscA"
                     /locus_tag="VFQ79_03930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEU0138829.1"
                     /translation="MSSMFQIDFGQPARSRVVGIDLGTTNSLVAYMDLTAPKVIPDAG
                     GSLIVPSVVSLSGTGELVAGNEARQMLLTDPERTVYSVKRLMGKGAGDVQDELRLFPF
                     HIASASDAVIQIELGDRTFTPPEISAQILRQLKTNAEAYFGERVTQAVITVPAYFNDA
                     QRQATKDAGRIAGLEVLRLVNEPTAAALAYGLDKRKEGIVAVYDLGGGTFDISILKLH
                     QGIFEVLATNGDTHLGGDDIDNLLLRIALEDTAAEWGEDLSRNHEAIQLLRRAVIEAK
                     EQLSTVPATSIRLEYHGKKYERELTRELFEQLITPIVDRTLAPCRNCIHDAGLNVEQI
                     DEVVLVGGSTRIPLVRQAVEKLFRAKPHTELNPEQVVALGAAVQAAILAGDVTDKLLL
                     DVTPLSLGIETMGGVVSKLIHRNSTIPASATEVFTTAVEGQRNVLIHVVQGERELARD
                     CRSLARFDLKDIDPMPAGMARIEVRFLIDANGILNVTARDIRTGKEQSMEVKPSYGLT
                     DEQVEAMIRESVDLAEEDFKARQVREAQVEADTILTAVEKARENEAWLELDDEERGRI
                     ERAVNELLMAYHADDHLLIRAQIDKLNTVTMKLAENMMNTAVRSALKGTKI"
     gene            complement(4645..5199)
                     /gene="hscB"
                     /locus_tag="VFQ79_03935"
     CDS             complement(4645..5199)
                     /gene="hscB"
                     /locus_tag="VFQ79_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEU0138830.1"
                     /translation="MQRPTPDYYRFLGLERRLSLDSKDLRDRFYNLSRLLHPDRYLRR
                     TPKERQYSLEATAILNDAYRVLRDHVSRAEYVLKEEGFNIGEQRSENVPPELLEEVFE
                     LNMALEELRSGDSSARPQLCEAHARFVAMQDEADSRLSDLFRRYDTTGDREALAEIRG
                     VLNRRKYIRNLVSEVEKELAASAA"
     gene            complement(5265..5585)
                     /locus_tag="VFQ79_03940"
     CDS             complement(5265..5585)
                     /locus_tag="VFQ79_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU0138831.1"
                     /translation="MISVTPKAVSKIKEAFAKQGVSGGLRLGVLGGGCSGLSYQFKFD
                     TKSRPTDNVFQFDGVEVFIDPKSMVYLDGMTLDYKESIIQSGFVFENPNAKKSCGCGT
                     SFSA"
     gene            complement(5613..6029)
                     /gene="iscU"
                     /locus_tag="VFQ79_03945"
     CDS             complement(5613..6029)
                     /gene="iscU"
                     /locus_tag="VFQ79_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEU0138832.1"
                     /translation="MAYSNKVLDHYNNPRNVGSLDKSDPNVGTGMVGAPECGDVMKLQ
                     LKVDPNSGIIEDAKFKTFGCGSAIASSSLATEWIKGKTVDEALQIKNTDIVNELSLPP
                     VKIHCSVLAEDAIKAAISDYKKKAGTNRQPVSEAVS"
     gene            complement(6063..>6435)
                     /locus_tag="VFQ79_03950"
     CDS             complement(6063..>6435)
                     /locus_tag="VFQ79_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0138833.1"
                     /translation="TELDEVYVNGSLEHRLPHNLNISFAYVEGESLLMGINDVAVSSG
                     SACTSATLEPSYVLKALGAGDDLAHSSIRFGLGRFNTEEEIDYVGNKVVEVVRKLREL
                     SPLYEMAKEGVDLAKVQWSAH"
BASE COUNT         1230 a         1958 c         1838 g         1409 t
ORIGIN      
        1 tggaggaact ggccgcggcg gtccatggcg tggcggatga gcttgggcgt ccggcatacg
       61 tgattgccga gagcgatctg aacgacaacc ggctggtgcg gcttcctgct gacggagggt
      121 acgggctgga cgcgcagtgg aacgacgatt ttcatcattc gctgcacaca ctgctcacga
      181 aggaaacaag cggctactat gccgactttg gcggcctcga tcatctggaa cgggcgatcg
      241 ccgaaggatt cgtctatgcc gggcagtact cctgctatcg cggccggtgc cacggaacgt
      301 cttcggtgga tctcgcggcc gatcgcttca tcgtcttctc gcagaatcat gaccagattg
      361 gcaatcgaat gatgggcacc cgcctcgcga acatgatcag cttcgaaagc gcgaaactgg
      421 ctgccggggt ggtgctgctg tccccattca tcccgctgct gttcatgggc caggagtatg
      481 ccgagacggc accctttctc tacttcgtaa gccatacgga ccccgatctg gtgcgggctg
      541 tgcgggaggg gcgcaaggac gaattcgctg cgtttgcgtg gcagggtgag ctaccggacc
      601 cgcaagctga agagagcttc ctccaatcga agctcaatcc ttcgttgcgc aacacgggcc
      661 gccatcgcgt gatgtgggag ttctaccgga atttgataga actgcgaaag agcaccacgg
      721 cccttgcggt gctcgataaa aactccctcg agctgagccg cttcgatgac agcttatctc
      781 tcgccatgcg ccgctgggcg cagcaggacc aggcgctcgt cgtcttcggt ttcagcgaat
      841 ccgaacagac aattcaggtg gcgctgccgc ccggaacgtg gtccaaagcg ctggattccg
      901 cgggtacgga gtggatgggg accggaagca ccgcgccgga gcatctcgaa tccaatggcg
      961 cggccacctt ccgtcttcgc ggcgaaagct ttgtggttta ccggttgaag gcttccggat
     1021 agttcggcga ataggtctgc ctctccggcc gcagccccaa ctttccgttg accacccaag
     1081 ggtcgccccg gacggcaaga tagtttatca tcttgtcgcg ccacgtcttc aactcgcctg
     1141 agctgccaac atcgcccgcg agatttttca tctcatgccg gtcggattcc aggttgaaca
     1201 actgctcctc tccgtcgcgc gcgtgaaaaa tatatttcat gcggccgtcg gtcaacgcgc
     1261 tccagtggtt ctcgggactg tagcagacgt cgtgttccag atcgatttcg ctgcgccatt
     1321 ccttaccgcc gttttttacc agcgatagca ggctggctcc atcgagcggt cgcgcggggc
     1381 tggagcccgc ggcatccaga aaagtaggca gcagatctcg caattcgacg gggtttgcca
     1441 tcacctgtcc ccgttttgcg gaaagcattc cagcaggcca gcgcatcagc atcggaacgc
     1501 gggcggacgg ctcataagcg tacgacttgc gccagagatt gtgatcgccg gtcatgtcgc
     1561 cgtggtccga cgtgtaaagg atcagcgtct cttccaacat gctgcgcttc tccagcgcat
     1621 cgagaattct gccgatctgt tcatcgatga aactgacgga gccgtagtag ccctgccgtg
     1681 aactccgaac ctgcgcgggc ctcaggttgc cgcgccagat atcgtaacga tcgctgttgc
     1741 gttcgcggta ccgcccggcc cagggtgcga catgcgcttc cgggaggtcc gcatcggcat
     1801 acaggtccat gaatcgctgc ggcggatcgt aaggcgaatg cgggcgggcg aacgagactt
     1861 tcagaaaaaa cggctccggg cggcgatacc cttcgaggaa gcgcactgcg cagtcgccgg
     1921 tccatcgcgt cgggtgcagc cgttcgggca atacgtaagc tttggccgga tagtcgttcc
     1981 atccgattcc ggtcgcatcg ggatcgagac ccggcgcttc cgaccagaac cacgcgcgat
     2041 agtcgcttcg gaaatccggc gattccgcgc gtccggattc atccaatagc acctggtgga
     2101 atccgtgcga attgcgttgc gggtgccagt gcatcttgcc gatacccatc gtgtagtagc
     2161 ccgcctcccg catggcgcgc ggcatctcga cgggatactt ttcggcaaca cgcccgtagc
     2221 cgagcatgcc gtggttccat ggggacatgc ctgtcagcaa cccggctcgt gcgggagtgc
     2281 aagtcggagt ggaggaatag gcgcagcgga agcgcacgcc cccggcagcg atgcggtcca
     2341 ggtttggtgt gcgaacagcg cgattgccat cggcgcccag acaatcgccg cgatgctggt
     2401 cggccatcag gaagagtacg ttcggccgct tcgcggcggc gaccccgcgt ctcgaaagaa
     2461 ttccgaacgc accggccttc aggagccctc gtcgggtaag ttccatccgt cccataataa
     2521 aaaacccgga gacggctgtc cccgggtttg ggtgtcgaag cgcggcggct tattcctggt
     2581 tgtccttcca ctcctcgtac caggccatct gaatgccttc cagcttgcct tcggtcgaga
     2641 gagcgggatc gtcgctgaag ccgctaagct cggtaatcca tctgtgcatg tcggtgaaac
     2701 gaactgtcag aggatccagg tccgggtact tgtcgtatag ggccagtgcg atgttctcgg
     2761 tatcgtccca ggtcagtttg gccatcagat cttggttcct ttcagagcgc tccggacggc
     2821 ggtgttcatc atgttctccg ccagtttcat cgtgaccgta ttcagcttgt cgatctgcgc
     2881 ccgaatgagc aggtgatcgt ccgcatggta cgccatcagg agttcattca ccgcccgttc
     2941 gatccggccg cgttcttcgt catccagctc tagccaggct tcgttctcgc gagccttttc
     3001 gacggctgtc aggatggtat cggcttccac ctgggcctcc cggacctgcc gtgccttgaa
     3061 gtcctcctcg gccaggtcga cggattcgcg aatcatcgct tccacctgtt cgtcggtgag
     3121 cccataagac ggcttcactt ccatcgactg ctctttccct gttcggatgt cccgcgcggt
     3181 gacgttcaga atgccgttgg cgtcgatgag aaatcgaacc tcgatacggg ccattccggc
     3241 cggcatggga tcgatgtctt tcagatcgaa cctggcgaga ctgcggcaat cccgcgcgag
     3301 ttcgcgctcg ccctggacaa catgaatcag aacattgcgc tggccctcga cggcggtggt
     3361 aaacacctcc gtggcactgg cgggaatggt ggagttgcga tggatcagct tggaaacgac
     3421 tccgcccatg gtctcgatgc ccaacgagag cggagtcacg tccagcagca gtttatcggt
     3481 tacatcgccg gccaggatcg ccgcctgcac cgccgcgccg agtgccacca cctgttccgg
     3541 attcaactcc gtgtgcggtt tggcgcgaaa gagtttctcg acggcctgac gcaccagcgg
     3601 aatacgcgtc gagccgccaa ccagcaccac ttcatcgatc tgttcaacgt tcaacccggc
     3661 atcgtgaatg cagttgcggc acggagcgag ggtccggtcc acaatcggcg taatgagctg
     3721 ttcgaacaac tcgcgagtca actcccgttc gtacttctta ccgtgatact ccaaccggat
     3781 gctggtcgcc ggaaccgtgg agagttgttc ctttgcttcg atgacagccc gccgcagcaa
     3841 ctgaatcgcc tcgtgattcc tggaaagatc ctcaccccat tccgcggcgg tatcttccag
     3901 agcgatccgg agcaacagat tgtcgatatc gtctccgccc agatgcgtgt cgccattagt
     3961 cgccaggact tcgaagatcc cctggtgcag tttgagaatg gagatatcga aggtgccgcc
     4021 acccaggtcg tagacggcga cgattccctc tttccgtttg tccaggccgt aggccagcgc
     4081 cgcggccgtc ggctcgttta ccaggcgcag gacttccagc ccggcgattc gtcccgcatc
     4141 cttcgtcgcc tgcctttgag catcgttgaa gtacgccggc acggtgatca ccgcctgtgt
     4201 cacccgctcg ccgaaatagg cttcggcatt ggttttcaac tgccgcagaa tttgtgcgga
     4261 gatctccggg ggagtaaacg tgcgatcgcc aagttcaatc tgaattaccg cgtcactcgc
     4321 actggcgatg tgaaaaggga acaaccggag ttcgtcctgc acgtctccgg ctcctttccc
     4381 catgagccgc ttcacggagt acacggtacg ttccgggtcg gtgagcagca tttgccgagc
     4441 ctcgttgccg gccaccaact cgcccgtgcc ggaaagcgag acaacgctcg gtacgatgag
     4501 cgaacctccg gcatccggga tcaccttggg cgcggtcaga tccatatacg ccacgaggct
     4561 gttggttgtg cccaggtcga tgccgactac tcgcgacctc gccggctgtc caaaatcgat
     4621 ttgaaacatg gaagacatgt gaattcacgc cgctgaagcc gcgagttctt tttccacttc
     4681 gctcaccagg ttccggatgt acttgcgcct gttcagtacg ccgcgtattt ccgccagcgc
     4741 ttccctgtcg cccgttgtgt cgtaacggcg gaataagtcg ctcaatcggc tatcggcttc
     4801 gtcctgcatc gctacaaacc gcgcatgtgc ctcgcacaac tgcgggcgcg ccgaagagtc
     4861 gccgctgcgg agctcttcaa gcgccatgtt caactcgaat acctcttcca gcagttccgg
     4921 cggaacgttc tccgaacgct gttcgccgat gttgaaaccc tcttctttga gcacgtactc
     4981 ggcccgcgag acgtgatccc gcagaacgcg gtaagcgtca ttgagaatcg ccgttgcctc
     5041 gagagagtac tgccgttcct tcggcgttcg ccgaagatag cggtctgggt gaagcaggcg
     5101 gctaaggttg taaaagcggt cccgcagatc cttcgaatcg agagaaagcc tgcgctccaa
     5161 cccgagaaat cgatagtaat ccggcgtggg ccgctgcaac gaattacaga attggcagaa
     5221 cagcgactcg tgctggggat gtccacattg ccagcaggct tccatcaggc tgaaaacgac
     5281 gttccgcagc cgcaactttt ctttgcgttt ggattctcga acacgaagcc ggactggatg
     5341 atggactcct tataatcaag cgtcatccca tccagataca ccatgctctt cggatcgatg
     5401 aacacctcta cgccatcgaa ctggaacacg ttatctgtcg gccgcgactt ggtatcaaac
     5461 ttgaattgat aactgaggcc cgagcatcca ccgccgagaa cgccaagccg gaggcctccg
     5521 ctaacgcctt gcttcgcaaa ggcctccttg attttcgata ccgctttagg agtaaccgaa
     5581 atcatctcta acgagttttt cctcccctat tttcaggata ctgcctctga aacaggttgg
     5641 cgattcgtgc cggccttttt cttgtaatcg gagatcgccg ccttgatcgc atcctcggcc
     5701 aggacggagc agtgaatctt taccggcggc aggctcagct cgttcacgat atcggtgttt
     5761 ttgatctgga gcgcctcatc cacggttttc cccttgatcc actcggtggc gagtgacgaa
     5821 ctggcgattg cgcttccaca gccaaaggtc ttgaacttgg cgtcttcaat gatgccggag
     5881 ttcgggtcga ccttcagttg cagtttcatc acgtcgccgc attcgggcgc gccaaccatt
     5941 ccggtcccca cgttgggatc gctcttatcc agtgaaccca cattccgggg gttgttgtaa
     6001 tgatcgagaa ctttgttcga ataagccatc tgctaatccc tttctattcc ctcgagagag
     6061 tcctaatggg cggaccactg cacttttgcc aggtcgaccc cttccttggc catttcgtaa
     6121 aggggcgata gttcgcgcag ctttctaacc acttccacca ctttgttgcc gacgtaatcg
     6181 atctcttctt ccgtattgaa acggcccagt ccgaagcgga tggaggaatg ggccaggtcg
     6241 tcgccggctc ccagagcctt cagcacgtag ctgggctcga gggtcgccga tgtacaggcg
     6301 gaaccgctcg ataccgccac gtcgttaatt cccatcaaaa gggattcgcc ctccacgtag
     6361 gcgaagctga tattcaggtt gtgcggcagt cggtgctcca gggagccatt cacgtacact
     6421 tcatccagct cggtt
//
LOCUS       DASTMK010000099        11257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_324997, whole genome shotgun sequence.
ACCESSION   DASTMK010000099 DASTMK010000000
VERSION     DASTMK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324997"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VFQ79_03955"
     CDS             <1..134
                     /locus_tag="VFQ79_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU0138834.1"
                     /translation="GLNNKAKVTMRKSYGFRTYRTLELALYHSLGKLPEPESTHEFF"
     gene            160..2703
                     /locus_tag="VFQ79_03960"
     CDS             160..2703
                     /locus_tag="VFQ79_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138835.1"
                     /translation="MIDLEPRFSIRSVPTAEQRLGDFSQTTAPSGSTRAPVTIYDPLT
                     TDPLTGQRQPFPGNRIPENRISPIARNVLQYVALPNLPESPAQATGLMNYIPDIPTED
                     RIHSAVTRIDHQFSARHRVFATLRWNHWEESIGDAFGNIATGQLATRINRGIGLDDVY
                     VFGPATVLNVRYGLARWESPTVGNSLGFDPAQLGFSPSVVAGLPLRSFPSFSVAGGLG
                     GTPTDYTITNNHSWIGALTRTQGRHTLNTGVQFMVLQTATFNAGAGAGSYNFTPQFTQ
                     RDFQTADRFSGFDTASFLLGYPASGQVSFNTSGFYSQRYLGLYLQDDWRVTSRLTLSL
                     GLRWDVEMPQRERFLRSNRGFDTSTPSPVEAAAQAAYARNPIPELPTSQFRVLGGQMF
                     AGVNGQPDRIFDTDYRTWQPRAGLAYRIDSKTAIRTGFGLFTSKTIDRGGQLGYSLDT
                     PFVATTDGGRTPANNLSNPFPQGFLSPVGSSLGLATSLGQQPRWDDPARRLPFSIQTS
                     FTVQRELPGNWLVEAGYSYNKSKRLAVGVPSNQVPLNRFLELGKPRYDASGALLAQPF
                     RLEDRVPNPFQGLPGFTGTALGTGATTSVLQLMSAFPQFTAFNRGLVDAGRSSYHGLQ
                     LKLEKRFSDTLSLILSHTWSKQLDYTTYLTILPNLGYQLDHSLNPDDRTHYFSAGWSW
                     ELPFGRGRRFFNSAPRIVTGVIGGWQLAGMYTLQSGRPASFGGTNLAWNGQDASLSGS
                     ERSLDKWFRTEYFGPVPKADTYALRTTPITFSTIRASRQNNADLAVFKNFRVKEKSTI
                     QLRAESFNALNHPRFGDPVTNPTSGSFGTVAKSQLNQPRIIQVALKVNF"
     gene            2709..3635
                     /locus_tag="VFQ79_03965"
     CDS             2709..3635
                     /locus_tag="VFQ79_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0138836.1"
                     /translation="MKLNRRSFVSSSAAALAGAQAASAQPPVSSVPELRVAACQILTF
                     PDTRQSAEKICSWIEKAASDKIDIVTFPEAAVCGYASDPEYWKAANPRDFEAAEATIV
                     ASARELNIAVVLGSAHWEDGKVYNSVLAIDKDGRVKGRYSKTFLAESWPTPGRNLPVW
                     TLAGVKSCFIICHDIRYPELVRLPAVAGAQICHYSSNESGLVEEHKLSAYRAMPISRA
                     TENSIYLVMANAPADPANMRAQEQSHGNSKIIHPNGTVLVEAGYFKETLVAATIDIKA
                     ADRSMARRAAREDGMIGRWLREGCKLVEGDGS"
     gene            3625..4449
                     /locus_tag="VFQ79_03970"
     CDS             3625..4449
                     /locus_tag="VFQ79_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0138837.1"
                     /translation="MVLRYLLLAIALVAPMMPQAPQFVVAGLQVVPDRWDKQSNLKKL
                     DKFAREAAAAGASLVITPEGFLDGYVGNDRDLDKLRYFDVAEPVDGPSLRRVRDLARE
                     LKIYLAVGFSEKRGDRVYNSVVIFGPGGELVSRYSKTHCGGEPHNTEGTEFPVVSTPL
                     GRWGTLICLDRQLPETSRILAIKGAQIILNPSYGMHGEINDVMMRTRAYENAVYVVFV
                     HPQRCLIIDPKGTIVAADNGKDDQIVTGKIILDDRIGKGPIRARRPEIYGELLNRK"
     gene            complement(4464..5519)
                     /locus_tag="VFQ79_03975"
     CDS             complement(4464..5519)
                     /locus_tag="VFQ79_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0138838.1"
                     /translation="MATIHDVAKRAGVSIATVSGVLNGSRYVSPPLIKRVQQAVKELD
                     FTINQVARNLQCRSTHMIGMLVPDISDPFHANVVRVVEDALKTAGYMLLLGNLRDRPE
                     EQARYLQILRSQQVDGLLIYMVPGFEEEIRKLVESRRAIVFMGREPETFKADLVGIDH
                     FNGTRMAVEHLISKGHRKIGIIPGPLGQPFSQSRIRGWQKTLQAAGLEADERYISFGD
                     YTIAGGQAAVAPLLDLPQPPTAILAGNFHVVIGVLQVLRQRKIARPQQMEVISSHDSD
                     VYDAFDPPLSSVAQPVHELGTKATELLLRRIRQPGRPPEHVLLRPRLHIRHTAPAVPA
                     TPASPGRKTRVKAAQAF"
     gene            complement(5579..8746)
                     /locus_tag="VFQ79_03980"
     CDS             complement(5579..8746)
                     /locus_tag="VFQ79_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138839.1"
                     /translation="MIRGALASALWIAACTGTALGQLTTAEIAGSVRDSQGLAVPSAM
                     VNVTNQGTGQQFTAAMQETGDFLLRSLPVGQYSMDVEAAGFKKFRRENIRLTAGQVAR
                     VNVQLEIGALTEAVTVTSDVPPVDTQTGTLATLVDERRVVDLPLNGRNVLSLASLTPG
                     INRVATANGPSSGQQSINVNGNRTDATNVMLDGASMYYAHRGAATTQPPPDAVQEVKI
                     ATNGIEAEFKRGAAAITTITRSGTNEFHGSVWNYMRNDAFDARSFFAARVSKLRYNQF
                     GAAGGGPIRRNKAFFFASYQGLRNPSDQLVSSAFPPTAAERAGDFSNTIGRQPNDPLT
                     NQPFPGGVIPSSRMDPIALKLLERIPEPNRSNGNYVIQVPRPNNGKMIMGRVDYDFTP
                     GDRTSVRYFIDNPENENAFPNGSNVDGYGHSTLGDRSQNTTVSHVHTFSPSVIMNVRA
                     SYGRFRYYNTNTVRETLSSLGARFIVAGGPGSLPLLTVPGRFTAGAEREGDRISDTYE
                     IGGTVSWFRGRHEVKFGADVQKFRFYFGNADRSNGEFTFDGSFTGNALADYVLGQPVE
                     MWQQSFKDNDTRYYAPGFFVQDRWRATPKLTLSFGFRWDIITPWRMVNTAAFSLVPGA
                     TSQYIPAAPAGILYDRDSGYPHKFDGVNPAPRIGFAYDVFGNGKTSVRGAYGISYVPL
                     IGQMANQNAQPFGFDVRTRNVGPLSDPYRFIDNPFGSSVDLTNPVYSLPISMAGSWAG
                     ETHTPYVQNFNFTIEQQVAPDTAVQLSYVGSLGRFEGTVREQNPAVYIPGNSTTQNTD
                     SRRLYAPVFGSITGYAADGNSSYHALQIQVSRRFQKGLTYSAYYTFSKAIDEASRGDA
                     ANNWNLQDPFDRRQNRALSDLHRAHRFVSSWVWELPFWRQQRSLVAKILGGWQFAGIA
                     TFSAGAPVTVTAGRDNSLTGAGNNRPDVLGDPHLSADRPRAEKLQRYFDTTRFVHNQP
                     GTYGNAARNILIGPGLIVFDLSMGKHFAITETRRIEFRWDAFNAFNRPNFNAPGANLN
                     APSTFGRITGAGAGRIMQGALRFEF"
     gene            8908..10977
                     /locus_tag="VFQ79_03985"
     CDS             8908..10977
                     /locus_tag="VFQ79_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0138840.1"
                     /translation="MKSFSYLVRHILMRRRWTASLLATALMPSLLAAQAARPRLGPWE
                     QATPRELGMDESRLAAARDFALQGAGSGIVIHRGKQVYSWGDLKAKYDLKSSTKSIGV
                     TVLGLALKDGKVQLSDPAVKHYPEFGAPPESNRTSGWLQQITLMHLAAQTAGFDKPGG
                     FQPLLFQPGTKWAYSDGGPNWLADCLTLTDKRDLEELMFERVFTPLGITPDDLHWRDN
                     AYRPKTLGGIPRREFGSGVHANVDAMARIGYLYLRAGRIHNSQVLPPEFVALVRQPVP
                     STKGLPVVHPEGYPDATSHYGLLWWNNGDGSLAGVPRDAYWSWGLYDSLIIVVPSLDL
                     VVARAGRGFDDSRGAIYSRLRPFIENLALAVDPVRAQLKPPYPPSAVIEEIEWAPVDT
                     IRRQGSDCDNFPSTWADDDNLYTAYGDCRGFFPLRPRKLGLGFAKIIGDPAGFQSVNI
                     PADAENFSTGAHGKKASGLLMVDGVLYLLARNAGNSQLAWSTDHAKTWTWADWKFTTS
                     FGHPTFLNFGKNYAGARDDYVYVYSPDHDSAYEQAAGIVMARVRKDRIRERGDWEFFK
                     SLDKNGQPVWTKDIAERGHVFSNPPAGVYRTQVTYNSALKRYFLNHILLGSEHVRFQG
                     GFGIYDAPEPWGPWTTVYYTPTWDTGPGENQHFPPKWMSADGRTMYLIFSGNDIFSVR
                     KATLKLR"
     gene            complement(11128..>11257)
                     /locus_tag="VFQ79_03990"
     CDS             complement(11128..>11257)
                     /locus_tag="VFQ79_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEU0138841.1"
                     /translation="EIERLFRRLKGFRRIFSRFEKLDVLFLGFLSFALIVEALRVV"
BASE COUNT         2296 a         3332 c         3244 g         2385 t
ORIGIN      
        1 aggggctgaa caacaaggcc aaagtcacta tgcgaaaatc ctacgggttc cgcacctacc
       61 gcactctcga actcgccctc tatcactcac ttggcaagct gccagagccc gaatccaccc
      121 acgagttttt ctgacgaacc cttctttaac tacgaaggca tgatcgatct cgaacccagg
      181 ttcagcattc gttccgtccc gacggcggag cagcggctgg gtgatttctc acaaactacc
      241 gcgccatccg ggagcactcg cgcgcccgtc acaatttacg atccgttgac aaccgatcct
      301 ctcaccggcc agcgccagcc cttccccgga aataggattc ccgaaaaccg gatcagtcct
      361 atcgcccgga acgttcttca gtatgtcgcg ctgccgaatc tgcccgaaag cccggcacaa
      421 gctaccgggc tgatgaacta tattcccgat ataccgacag aagacaggat tcattcggca
      481 gtgacgcgaa tagaccacca gttcagcgcc cggcataggg ttttcgcgac actacgatgg
      541 aatcattggg aagagtcaat cggcgatgcc ttcggtaaca tcgccacggg acaattggct
      601 acccgcatta atcgggggat cgggctcgat gatgtatacg ttttcggccc ggccaccgtg
      661 ttgaatgtgc gttatggcct ggcgcgttgg gaaagtccga cggtcggcaa cagcctgggc
      721 ttcgatccag ctcaattagg cttttcgccc tcggttgttg ccgggctccc acttcgctcc
      781 tttcccagct tctctgtcgc cggaggcctg ggaggaaccc caaccgacta caccattacc
      841 aacaaccata gctggatcgg cgctcttact cgaacgcagg gccgtcatac tttgaacacc
      901 ggcgtgcagt tcatggtgct gcaaacggcc acgttcaacg caggagcagg tgccggaagt
      961 tacaacttca ctccacaatt tactcaacgc gacttccaaa ccgcggaccg tttctccgga
     1021 tttgatacgg cctcctttct tcttggttat cccgcgagcg gtcaggtgtc cttcaataca
     1081 agtggtttct acagtcaacg atatctggga ctctatttgc aggatgactg gcgagttaca
     1141 tcgaggctga ccttgagtct cggcctgcgc tgggatgttg agatgccgca gagggagcga
     1201 ttccttcgtt ctaaccgcgg ttttgatact tcaacgccca gcccggtaga agccgccgcg
     1261 caagccgcct atgctcgaaa tcctattccc gaactgccca cgtcccagtt ccgcgtcctg
     1321 ggtgggcaga tgtttgccgg cgtcaacggc cagcctgatc ggatctttga tacggattac
     1381 cgaacatggc agccgcgcgc cgggcttgcg tacagaattg attcgaaaac agcgattcgg
     1441 actgggtttg gtttgtttac atcgaagacg atcgatcgag ggggacaact cggatacagc
     1501 ctggacacgc cttttgttgc tacaacggac ggaggtagaa cgccggcgaa taacctgagt
     1561 aatccatttc cccaaggatt cctctcgccc gtcggcagtt ccctcggtct cgcaacaagt
     1621 cttggacagc agcctcgctg ggacgatccg gcacggcgcc tgccgttttc gattcagaca
     1681 tcctttaccg tgcagaggga gttaccgggt aactggctcg tggaagcggg atactcctac
     1741 aacaaatcga agcggctcgc cgtcggcgtt ccttccaatc aggtgcctct caatcggttt
     1801 ctggagttgg gcaagcctcg ctacgacgcc tccggtgcgc tgctggccca acccttccgc
     1861 ctagaggatc gtgtgccgaa tcctttccag ggtctaccgg ggttcaccgg aacggctttg
     1921 ggaacgggcg cgaccacatc ggtgttacag ctcatgtccg cctttccgca attcactgct
     1981 tttaatcgcg gactcgtaga tgcaggccgt tcgagctacc atggtcttca actcaagctg
     2041 gaaaagcgtt tctccgacac gctttcgctg attctgtcgc acacctggtc gaagcagttg
     2101 gactacacaa cgtatctgac gatactgccg aatttgggct atcagctcga tcactccttg
     2161 aatcccgatg accggacaca ttatttttcg gcgggatggt cgtgggaatt acctttcggc
     2221 cgcggccggc ggttcttcaa cagcgctccg cgaatagtga ccggtgtaat cggtggatgg
     2281 caactggctg gtatgtacac gttgcagagc ggccggccgg cttccttcgg cggtaccaat
     2341 ctagcctgga acgggcagga tgcctcgctt tccgggagtg agcgatcgct ggataaatgg
     2401 ttccggacgg agtacttcgg accggttccg aaagctgata cgtacgcttt gcgcaccacg
     2461 ccgatcacgt tctccacgat ccgcgcgagc cgccagaaca acgccgatct ggcggtgttc
     2521 aagaatttcc gggtcaaaga gaaatcgacg attcagctcc gggcggagtc cttcaatgct
     2581 ctcaaccatc cccgcttcgg cgatccggtt acgaatccca caagcggcag cttcggaacc
     2641 gtcgcaaagt cgcagctcaa tcaacctcgt attattcagg tggctctcaa ggtgaacttc
     2701 tgaatgccat gaaactcaac cggcgttcct tcgtttcctc gagtgccgcc gcactagccg
     2761 gcgcgcaagc ggcttctgcc caaccgccgg tctcttcggt tcctgaattg cgcgttgcgg
     2821 catgccagat tctcacattt ccggatacca ggcaaagtgc ggagaagatt tgctcctgga
     2881 tcgaaaaggc tgcgagcgac aagatcgaca tagtgacatt tcccgaggcg gcggtgtgcg
     2941 gctatgcatc ggatcctgaa tactggaagg ccgccaatcc ccgggacttc gaagcggccg
     3001 aagccaccat tgttgcttcc gcccgtgagc tgaatatcgc cgtcgttctt ggcagcgcac
     3061 actgggagga cggaaaagta tataacagcg ttctcgccat cgataaagac gggcgggtga
     3121 aaggccggta ttccaagacg tttctcgccg agtcctggcc cacgcccgga cgcaacctgc
     3181 cggtttggac gcttgccggc gtgaaatcct gcttcatcat ttgccacgat atccggtacc
     3241 cggagcttgt gcggcttcct gctgtggcgg gtgcgcaaat ttgtcattat tcgagcaatg
     3301 agagtgggct tgtggaggag cacaaacttt cggcataccg cgccatgccc atttcgcgcg
     3361 caacggagaa ctccatttac ctggtgatgg cgaatgctcc ggcggatccg gctaacatgc
     3421 gcgcgcagga acagtcgcat ggaaatagca agatcattca ccccaatggc accgtcctgg
     3481 tcgaagcagg atacttcaaa gaaacgcttg tcgcggctac cattgatata aaagccgccg
     3541 accgctctat ggcaagacgg gccgcgcgcg aagatggaat gatcggcagg tggctgcgcg
     3601 aaggctgcaa actggtggag ggtgatggtt cttagatatc ttttgctcgc cattgcgctt
     3661 gtggccccaa tgatgccgca ggctccgcag ttcgttgtcg ccggacttca agtcgtgccg
     3721 gaccgttggg acaaacaatc gaacttaaag aagttggata agtttgcgcg agaagccgca
     3781 gccgccggag catctctcgt catcacgccg gaaggttttc tggacggcta tgtcggcaac
     3841 gaccgcgatc tggacaaatt gcgatacttc gacgtagccg aaccggtaga cggtccttct
     3901 ttacggcgtg tccgcgacct tgcccgcgag ctgaagatct atctggccgt cggcttttcg
     3961 gagaagcggg gagatcgcgt ctacaactcg gttgtgattt tcggacccgg cggcgaactg
     4021 gtaagccggt attccaagac gcattgcggc ggagaaccgc acaacacgga aggcacggaa
     4081 ttccccgtcg tatctacgcc tcttggccgt tggggcacgc tgatctgcct ggaccgccag
     4141 ttgccggaaa catcgcgcat tctcgccatc aaaggcgcgc aaatcatact caacccatcc
     4201 tatggaatgc atggtgagat caacgatgtg atgatgcgaa cgcgcgctta cgagaacgcg
     4261 gtttatgtgg tcttcgttca tccgcagcga tgtctcatta tcgatcccaa gggcacgatc
     4321 gtcgccgcgg acaacggcaa ggacgatcaa atcgtgacgg gcaaaattat cctcgacgac
     4381 cgcataggaa aagggcctat ccgcgcacgc cgtcccgaaa tctacggcga gttgcttaat
     4441 cgcaagtaga gcggggcgct ttttcaaaat gcctgcgcgg ccttaaccct ggttttccgg
     4501 ccaggactcg ccggtgtagc tggtaccgcg ggagcggtat ggcggatgtg gagccgcggg
     4561 cgcaggagta cgtgctccgg cggccgtccc ggctggcgga ttctgcgcaa cagcagttcc
     4621 gttgctttcg taccgagttc gtgcaccggc tgcgcgacgc tcgacagcgg cgggtcgaat
     4681 gcgtcgtaga catcggaatc gtgcgaagag atcacttcca tctgttgcgg ccgcgcaatc
     4741 ttccgctggc gcagcacctg tagtacgccg atcaccacgt ggaagttgcc ggccaggatg
     4801 gcggtcggcg gctgcggcaa atccaacagc ggcgccaccg ctgcctgccc gccggcaatg
     4861 gtgtaatcgc cgaagctgat gtaacgctca tccgcttcga gcccggctgc ctgcaatgtc
     4921 ttttgccagc cgcgaatgcg cgattggcta aagggctgcc ccagaggtcc cgggatgatt
     4981 ccaatcttcc ggtggccttt cgagatcaaa tgctctacgg ccatccgggt tccgttgaag
     5041 tgatcgatcc caaccaggtc ggccttaaat gtttcgggct cacgacccat gaagacaatc
     5101 gctcgccggc tctccacaag tttgcgaatc tcctcctcga aaccgggaac catgtagatc
     5161 aacagcccat ccacctgttg cgaccgcagg atctggagat agcgtgcctg ttcctcgggc
     5221 cggtcgcgaa ggtttccaag cagcagcata tagccggccg tcttcagagc atcctctacg
     5281 acgcgaacca cgttggcatg gaatgggtcc gaaatgtcgg gcaccaacat accgatcatg
     5341 tgcgtcgacc ggcactgtag attgcgggcg acctggttta tggtgaaatc caattcttta
     5401 acagcctgtt gaacccgctt gatcagcggg gggcttacgt accgcgaacc gttcaacacg
     5461 cccgagacgg ttgctatcga gacacccgca cgcttcgcaa catcgtgaat tgttgccata
     5521 tgaccatctc accgcccctt cgcggtcgcg gctctgattc tgagccgcga gcgtccggtt
     5581 agaactcgaa gcggagcgca ccttgcatga tgcggcctgc tccggcaccg gtgatccgcc
     5641 caaacgtcga gggagcgttc aggtttgcac cgggcgcatt gaaattcggc cggttgaagg
     5701 cgttgaacgc gtcccaccgg aattcgatcc gtcgtgtttc ggtgatagcg aagtgcttgc
     5761 ccatggacag gtcgaagaca atcaggcccg gacctatcag aatatttcgt gctgcgttgc
     5821 catacgtgcc aggctgattg tgaacgaacc tggtcgtgtc gaaatagcgt tgcagtttct
     5881 ccgctcttgg ccggtcagcc gacaggtgcg ggtcccccaa cacatccgga cgattgttgc
     5941 ccgctccggt gagtgagtta tcccgcccgg cggttaccgt gaccggggcg ccggcgctga
     6001 atgtggcgat accggcgaac tgccatccac ccagaatctt tgccaccaag gaccgctgct
     6061 gccgccagaa gggcaactcc cacacccacg aactgacaaa ccggtgtgct ctgtgaagat
     6121 cgctgagtgc ccggttctgg cgccggtcaa acggatcctg caggttccag ttgttggccg
     6181 catcacccct gctggcctca tctatcgcct tcgagaaggt gtagtaggcg gagtaagtca
     6241 gacctttctg gaagcggcgt gatacctgaa tctgcaatgc gtgatagctg gagttgccgt
     6301 cggcggcata gcccgtgatg ctgccgaaga ccggcgcgta caaccggcgg ctgtccgtat
     6361 tctgtgttgt ggagttgccc ggaatgtaca cggccggatt ctgctcgcgc acagtgcctt
     6421 caaaacgccc cagcgatcct acatagctga gctggaccgc ggtatcgggc gccacttgtt
     6481 gttcgatggt gaaattgaaa ttctgcacat acggcgtgtg tgtctcgccg gcccaggagc
     6541 ctgccatcga aatcggcagc gaataaacag ggttggtcag gtctacagag cttccaaagg
     6601 gattatcgat gaaccggtat ggatcggaga gcggaccgac gtttcgggtg cggacgtcga
     6661 agccgaacgg ttgcgcgttc tgattggcca tctgtccgat cagcggcaca tagctgatgc
     6721 cgtatgcgcc ccgcacgctg gttttcccgt tgccgaacac gtcgtaggcg aatccaatgc
     6781 gcggcgccgg gttgacaccg tcgaacttgt gcggataccc ggagtcgcgg tcgtagagga
     6841 tgcccgccgg agctgcggga atgtactgcg acgtggctcc aggtacgagt gaaaacgcgg
     6901 ccgtgttgac catcctccaa ggtgtgatga tatcccagcg gaaaccgaag ctgagcgtca
     6961 gctttggtgt cgcgcgccac cggtcctgca cgaagaaacc tggcgcgtag taacgcgtgt
     7021 cattgtcctt gaacgattgc tgccacatct ccactggttg gccaagcacg tagtctgcaa
     7081 gggcgtttcc ggtgaagctg ccatcgaacg tgaactcacc gttggagcgg tcggcatttc
     7141 cgaaatagaa acggaacttc tgcacgtctg ctccgaattt gacctcgtgc cggccgcgaa
     7201 accagctcac ggtgcctccg atttcgtacg tgtcgctgat acggtcgccc tcccgctcgg
     7261 ctccagcggt aaagcgtccg ggaaccgtga gcagtggaag gctacccggc ccaccggcga
     7321 cgatgaagcg agcgccgagg ctcgacagcg tctcgcgaac cgtgtttgtg ttgtagtaac
     7381 gaaaccgtcc gtagctggcg cggacgttca taatcaccga aggactgaag gtgtgtacat
     7441 gcgagacagt ggtgttttgt gaacggtcac ccaaggtgga atggccgtag ccatcgacgt
     7501 tcgagccgtt cgggaaagcg ttttcatttt cgggattgtc aataaaatag cgcacgctgg
     7561 tgcggtcgcc gggcgtgaaa tcgtaatcga cacggcccat tatcatcttg ccgttattcg
     7621 gccgcggaac ctggatcacg tagttgccgt tggatctgtt aggttcggga atccgctcga
     7681 ggagcttcaa tgcaatcgga tccatccggg aagaaggaat gacgccgccg ggaaacggct
     7741 ggttggtcag cggatcattc ggttgccgcc cgatggtgtt gctaaaatcc ccggcacgtt
     7801 ccgccgcggt gggcggaaat gcactcgaga ccagttggtc cgatgggttg cggaggccct
     7861 gatacgacgc aaagaagaaa gctttgttgc gccggatggg cccgccgccg gcggcgccga
     7921 actggttgta ccgcaacttc gaaacccggg cagcgaaaaa acttcgcgca tcgaacgcgt
     7981 cgttccgcat gtagttccac acggagccgt gaaactcgtt tgtgccgctc cgtgtgatcg
     8041 tggtgatcgc ggccgcaccg cgtttgaact cggcttcaat gccgttcgtc gcgattttga
     8101 cttcctgcac tgcgtccggc gggggctgag ttgtagccgc tccccggtga gcgtagtaca
     8161 tcgacgcgcc gtccaacatg acattcgtcg cgtccgtacg gttgccgttc acattgatac
     8221 tttgctggcc ggaagaaggc ccgttagcag tggccacgcg gttgatgccc ggcgtaagcg
     8281 aggcgagact cagaacattg cggccgttca gcggcagatc cactactcgc cgctcatcga
     8341 ccagcgtggc gagcgtgcct gtctgggtgt cgaccggcgg cacgtcggac gtgaccgtca
     8401 cggcttcggt gagcgcgccg atttccagtt gaacattcac tcgggcgacc tgccccgcgg
     8461 tcaaccggat gttctcccgt cgaaacttct tgaaccctgc cgcctcaacg tccatcgagt
     8521 actggccgac cggcagagaa cggagcagaa agtccccggt ctcctgcatg gcggcggtga
     8581 actgttgacc cgtaccctgg ttagtgacat tgaccatggc ggaagggaca gccaatccct
     8641 ggctgtcgcg caccgatcca gcgatttccg ctgtcgtgag ctgaccgagc gccgtccccg
     8701 tacaagccgc aatccaaagc gcggaagcaa gtgctcctcg tatcatacaa gacctcctct
     8761 aatgccgttc gcgagaagaa aacgttttct tcccagattt tggccgatta tgacagaggc
     8821 caacctgcat gtcaaaccgt gattcggcgc catagtcagt atggaattga cgcgtcatcg
     8881 cagggcttga tacgatacgt ttcgcttgtg aaatcgtttt cttatctggt tcgtcacatt
     8941 ctgatgaggc gccggtggac ggccagcctg ctcgcaaccg cgctgatgcc ttccttgctg
     9001 gcggcccagg cggcacgccc aaggcttggt ccctgggaac aagctacgcc gcgtgaactt
     9061 ggcatggacg aatcccgtct cgcggcagca cgggattttg cgctccaggg cgccggttcg
     9121 ggaatcgtca ttcatcgcgg caagcaggtc tactcctggg gcgacctcaa ggcgaagtac
     9181 gatctgaaat cttcaaccaa gtccatcgga gtaaccgtgc ttgggctggc gctgaaggac
     9241 ggtaaggtac aacttagcga tccggcggta aaacactatc ccgagttcgg cgccccgccc
     9301 gagagcaatc gcaccagcgg atggctgcaa cagatcactc tgatgcacct ggccgcgcag
     9361 accgcgggat tcgacaaacc cggcggattc cagccgcttc tattccagcc tggtacgaag
     9421 tgggcgtata gcgacggagg acccaactgg ctcgccgatt gtctcacgct gactgacaag
     9481 cgtgacctgg aagagttgat gttcgagcgt gtcttcacgc ctctgggcat tactccggac
     9541 gacctgcatt ggcgggacaa cgcctaccgt ccgaaaactc ttggcggcat cccccgtcgt
     9601 gagttcggct ccggcgttca cgcaaatgtg gacgcgatgg cccgcatcgg ctatctttat
     9661 ctgcgcgccg gccgtattca caactcccag gtcctgccgc cggaattcgt tgccctggta
     9721 cgacagcccg ttccttccac caaaggactg ccggtcgttc atcccgaagg ctatccggac
     9781 gccaccagcc attacggtct gctgtggtgg aacaacggtg acggatcgct tgccggagtt
     9841 ccgcgagatg cctattggtc gtggggcctt tacgattcgc tcatcatcgt tgttcccagt
     9901 ctcgatctcg tggtggcgcg ggccggccgg ggattcgatg attctcgcgg cgccatttac
     9961 tcgcgcctgc ggccgttcat cgagaatctc gcgctggctg tcgatcccgt gcgcgcacaa
    10021 ctgaaaccgc cctatccgcc gagtgcggtc attgaagaga tcgaatgggc tccggtcgat
    10081 acgattcgtc gccagggctc cgattgcgat aactttccgt ccacctgggc tgacgatgac
    10141 aatctctata ccgcgtacgg cgactgccgt ggcttcttcc ctttgcgtcc tcgcaagctc
    10201 ggtctcggct tcgccaaaat catcggtgat cccgccggct tccagagcgt gaatatccct
    10261 gccgatgccg agaacttcag tacgggcgca cacggcaaaa aagcgagcgg actgctgatg
    10321 gtggatggtg ttctctactt actcgcacgc aacgccggca attctcaact ggcgtggtcc
    10381 accgatcacg cgaagacatg gacgtgggcg gactggaaat tcacaacgag ttttggccac
    10441 ccgacgttcc tgaatttcgg caagaactac gccggtgcgc gtgatgacta cgtttacgtc
    10501 tattcaccgg atcatgacag cgcttatgaa caggcagcgg gtatcgtcat ggcccgggtt
    10561 cgaaaagacc ggattcgcga acgcggcgac tgggaattct tcaagtcact cgataagaac
    10621 gggcagccgg tctggacgaa ggacatcgcc gaacgtggtc acgtgttttc gaatcctccg
    10681 gcaggcgtct accgcacaca agtcacttac aacagcgccc tgaagcgcta cttcctgaat
    10741 catatcctgc tcggcagcga gcatgtccgc ttccagggcg gctttggcat ctacgacgct
    10801 ccagagccct ggggaccctg gaccacggtt tactacacac ccacctggga caccggcccc
    10861 ggagaaaacc agcactttcc gccgaagtgg atgagcgccg acggccggac aatgtacctc
    10921 atcttctctg gaaacgacat cttttccgtt cgtaaagcga cgctcaaact ccgatagcaa
    10981 gcggcgccgc ctcagtctgt ttgtggaagt ggagcgtatt aacagaacgc aattccctgc
    11041 agcggatgtg cttcgtgaaa ccgtgcggtg ctcactcgcg gagaatttaa gtaaggctgg
    11101 ctttcaactc ctcacgctag ggcgtgttca cactacgcgc aatgcctcga cgataagtgc
    11161 gaatgaaagg aaccccagga acaggacatc gagtttctcg aagcgcgaga agatacgacg
    11221 gaaacccttg agccggcgga acaggcgttc gatctca
//
LOCUS       DASTMK010000100         8182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_357457, whole genome shotgun sequence.
ACCESSION   DASTMK010000100 DASTMK010000000
VERSION     DASTMK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357457"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFQ79_03995"
     CDS             <1..785
                     /locus_tag="VFQ79_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0138842.1"
                     /translation="EYLLGWSSESGSREYFDVHRARLLKTLEITPPGEPGQSVLEMGA
                     YFQITPALKTRLGYEEVRGCYYGAAGTTEHRKAVSLEGEEFECDLELFDAEKDIFPYP
                     NETFSTVLCCELLEHLFEDPMHMMSEINRILKVGGHLVLTTPNICSLRAIAAILTGYH
                     PGFFPAYILPKEGEEPDPRHNREYAPREIAMLLTDAGFEVKRLETGPFRDAPRPELGW
                     VSHLLEYHSLPGELRGDGIYAVGRKSGPVRERYPEWLYSGGG"
     gene            782..2011
                     /locus_tag="VFQ79_04000"
     CDS             782..2011
                     /locus_tag="VFQ79_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138843.1"
                     /translation="MSAGYVPLKIEAAAEEGVIALSCRLENQSAQHWKPSDGLALGWQ
                     VYDPESSLFLQEGEWIPLKENIPPGGYTDLAFRLQLPPYPGAYRIYISLRSPAGWFFA
                     RGLPMLVVDARVDEFGRAHPERTRVTTLGRLRLLKVPDSLRRMAKRFVSVLWGNRTLI
                     LSMVKRDLQARYRGSFGDVLWTVLNPVLLMATYFFVFGVVLQARFANDPSRAGFVLYF
                     LAGMLPWLAFSEAVGRAPASMLEHRNFIKKLLFPVDIVPVTQTLAALITEAFAVLIFI
                     VFVLLTRGSIQVTACWLPVILIPQVLFTLGAAWFFSALGVFVRDLGQIMGYVLTLWFF
                     LTPICYPETQIPAGAMGVLGKNPIYTLVRAYRAIFLENQAPAFASMWKLWLLSVLVCA
                     FGFVWFQKLRKTFADVI"
     gene            2050..2436
                     /locus_tag="VFQ79_04005"
     CDS             2050..2436
                     /locus_tag="VFQ79_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEU0138844.1"
                     /translation="MKMGLVVFAHGSRIESANEAVRMAAVALAREGGYELVVPAFLEL
                     GTPDLATAIGILVKQGANRIAVIPYFLTLGLHVDEDLPRIVDGISRIYNGVKIHVTAP
                     LDGHPALVRVLLDRAREAVESSRGGE"
     gene            2423..3151
                     /locus_tag="VFQ79_04010"
     CDS             2423..3151
                     /locus_tag="VFQ79_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0138845.1"
                     /translation="MAANEVIEFGRFTLRARLIEYRDLACDVRHFVFEVPETERLTFA
                     PGQFLSFMEQLEGKMVTRAYSIASPPDGNRFDLCLNRVKEGLFSPHLFHLEPGDEVEA
                     KGPYGTFALRQPASDSVMIATGTGIAPFRSILLSQVPSDTEHQYTLLFGVRYEETLLY
                     REEFEALGNTHPNFRFWPTLTRPEPKWTGRVGRVQQHLLEALEERRDVDVYICGLKEM
                     VNDIRGRLKELGFERQRVIYEKYD"
     gene            3168..3386
                     /locus_tag="VFQ79_04015"
     CDS             3168..3386
                     /locus_tag="VFQ79_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138846.1"
                     /translation="MPSGSARIFAMAVLAMVVFAATTGLLMFALPAPLSSTDYLVIGS
                     AATLVSLSTVFAVAALSGRSLQKERKIR"
     gene            3610..5868
                     /locus_tag="VFQ79_04020"
     CDS             3610..5868
                     /locus_tag="VFQ79_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138847.1"
                     /translation="MAAIAGPVTAQNAGSNPPAIDFTPNQGQWSPQFTIAGFTRPDGA
                     APVVYDFARGRDGEVVATGMFRWAGTRRAGPVARLEGDEWRSVRDAWPREGEVAFSAI
                     AINNEGVTALSTYGGPFGSVPVEIWLDTGEEVRVIGNLQGAVRSLAWFDGKLWAAGFF
                     QMGQGGVSNLAVWDGNTWSAPPGGPADRAVYRLVSGNSLLLAGDFTTVGGIPARGIAE
                     WNGNTWKAYDLNLPIAAAHVYAVARGQSGELYAGGAVTGGVLRWNGTGWEQLGGGVFQ
                     RDTNGVISDLTTHQGELYVTGCFTSLNGPESDPSAIRAQSVAKWSGSQWESLDDGSDL
                     LTHGWFEWGVCGDEPNQFTIWDVRYQRLFSDGERVFLGGMMPGVAGIPSQSIVSYDGN
                     EWRGYGETQDGLFGASQTVIAGGPGRNVYMTGFVSHAGQAQSRASVYRYQNGWEAVAP
                     PWPQGLICNRIAVDPWGRLFAGCYESNQSGENQQPIVLGLNGGEWAPLGVLELPGQIW
                     DMGVDHWGRLWVVGGVRLGPAEGSGFVARWDGDQFTIVEDRFNSLVFQIDFAPEEAGS
                     PGDVMVAGAFTRIGAESFERIAQWRGGRWEPLGAGLGSAVLALAWGRDGVYISMEAPS
                     SSEEPDTPRFLLGHWDGSRWVELATPENGLPAPRENTVHQFRDLLAVGRYVIAAGSVW
                     PETGGRNVFVYDGQRLAPLGGGLNAIGVSSIALANDGLWFAGEIAEAGSGDQVIPSVG
                     IARFTAPVESEP"
     gene            complement(5920..6765)
                     /locus_tag="VFQ79_04025"
     CDS             complement(5920..6765)
                     /locus_tag="VFQ79_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0138848.1"
                     /translation="MNARLFPLLFVCALSLMNCSSPSSQVHHNQLTSEERSAGWRLLF
                     DGKTLAGWEDPAAKSPAGDAWVVKDGCIKAVEKPRTREDLFTIDQFGDFELAFDWKIS
                     SGGNSGVKYRIQDRVSLIKGKLNPDARRFEDTVDYELLHRASSRSTIPPGANYEEYAI
                     AFEYQLIDNDKHPDAGRNADRSAGSIYSLVAPVKPVVRPAGEFNESRIILRGNHVQHW
                     LNGAKIVDTSLDSPEIAKQLAERWTTRSPVYELLTKQPKKQTSIALQHHVDEVWFRNI
                     KIRPL"
     gene            6838..7842
                     /locus_tag="VFQ79_04030"
     CDS             6838..7842
                     /locus_tag="VFQ79_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU0138849.1"
                     /translation="MSRILIFVLVAAAAAETHEIYPETHSRVFSAFKEPVLRVKSGDT
                     VITRTWDSGGQDYKGVRHIQHPYVYPESGNPLMGPFYIEEAEYGDALEVHLDKVRLNR
                     NWGYTSYRLIPGVLSNVEDLYRNFYKMDALRPGRADLIPWDIDLERGEASPRLLEGRG
                     FKVQVRPMLGCIGVAPPGEEVQTSGPSSTHGGNIDYNDIVEGVTVYFPVYHKGAFFYV
                     GDGHAVQGDGEGLGTGVETSLDVQFTVSVHKGKRLSIPRLVNKEYIVSIGSQPEFRSS
                     MDFGLRMANSDMIRWLTSEYKLTVPEAHLLMGTVVQHKIVTYFGTIATLIPRKYLPSR
                     "
     gene            7910..>8182
                     /locus_tag="VFQ79_04035"
     CDS             7910..>8182
                     /locus_tag="VFQ79_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138850.1"
                     /translation="MISVKLPTVSLCCILALVLVQEVSPDARTTPPVHVSPALAAALE
                     QITPDRLKGDVSFLASDALEGRATPSRGLEVAAEYIAARFRAAGLEP"
BASE COUNT         1593 a         2183 c         2582 g         1824 t
ORIGIN      
        1 cggagtatct gcttggttgg tcgagcgaat cgggcagccg cgaatacttt gacgtccatc
       61 gcgcgcgcct gctgaaaacg ctcgagatca cgccgcccgg ggagcccggc cagtcggttc
      121 tcgaaatggg tgcatacttt caaatcacgc ccgctctaaa gacgcggctg gggtatgagg
      181 aggtgcgcgg atgctactac ggagccgcgg gcaccacgga acaccggaag gctgtatcgc
      241 tcgaaggcga agaattcgag tgtgacctgg aactcttcga cgccgaaaag gatatttttc
      301 cctatcccaa cgaaacattt tcgacggtgc tttgctgcga attgctggaa cacctgtttg
      361 aggacccgat gcacatgatg tcggaaatta accgcatcct gaaggtcgga gggcatctcg
      421 tgctgaccac gccgaacatt tgttccctgc gcgccattgc cgctattctg acgggctatc
      481 atcccggctt tttcccggca tacatacttc ccaaagaggg agaagagccc gatccgagac
      541 acaaccgcga atatgcgccc cgtgagattg ccatgctgct taccgacgcc gggtttgaag
      601 taaaacgcct ggaaacgggt ccattccggg acgctccgcg gcccgaattg gggtgggtgt
      661 cgcacctgct cgaataccac agcctgccgg gtgaactgcg tggtgacggc atctacgcgg
      721 tgggcagaaa atcgggtccg gtgcgcgagc gttatcccga atggttgtac agtgggggcg
      781 gatgagcgcc ggatatgtgc cgctgaagat cgaggccgcg gcggaggaag gcgtcatcgc
      841 tctcagttgc aggctggaga accagtccgc tcagcactgg aagccgtcgg atggactggc
      901 gctcggctgg caggtctatg atcctgaatc cagcctgttc cttcaggaag gggaatggat
      961 tccgctcaag gagaatatcc ctccaggtgg gtacaccgac ctcgcgtttc gcctgcaact
     1021 tccgccgtat cccggcgcat atcgcatcta tatctcactt cgttcgccgg ccggctggtt
     1081 ttttgcacgc gggctgccga tgctggtggt ggatgcgcga gtggatgagt ttgggcgggc
     1141 tcaccccgaa cgcacacgcg ttacgacgct tggcagactc cggttgctca aggtgccgga
     1201 ttcgctccgc cgcatggcga aacgcttcgt atcggtactt tggggcaacc ggacacttat
     1261 tctctccatg gtgaagcggg accttcaggc tcgttatagg ggttcgtttg gcgatgtcct
     1321 ctggaccgtt ctgaacccag tattgttgat ggccacctac ttctttgtct tcggtgtggt
     1381 gttgcaggcc aggtttgcga atgatccgag cagggccgga tttgtgttgt actttctggc
     1441 cggcatgttg ccgtggctgg ctttcagcga agcggtagga cgcgcgcccg cttcgatgct
     1501 ggagcacaga aatttcatca agaagctgtt gtttccggtc gatatcgttc cggtgacaca
     1561 gacgctcgcc gcgctgatca ccgaagcgtt cgcggtgctc atcttcatcg tatttgtgct
     1621 cctgacgcga gggagcattc aggtgacagc ctgctggctt cccgtaatcc tgattccaca
     1681 ggttcttttc acgctgggag cggcgtggtt cttttcggcg ctcggagttt ttgtgcgcga
     1741 tctgggacag attatgggtt acgtgctgac cctgtggttt tttttgactc ccatctgcta
     1801 ccccgaaacg caaatccctg ccggtgcgat gggtgtactc gggaaaaatc cgatttacac
     1861 cctagtgcgt gcttacaggg ctatctttct ggaaaatcaa gcgccggcgt tcgcatcgat
     1921 gtggaagctg tggctccttt ccgtcctcgt ttgcgccttt ggcttcgtct ggtttcagaa
     1981 gctccgcaag actttcgccg acgtcatctg atgagaaaat agaattaacg ggtgcaggta
     2041 gaggggcgga tgaaaatggg actggtggta ttcgcgcacg gatcgcgcat tgaatccgcc
     2101 aacgaggccg tgcgcatggc ggcggtggcg cttgcgcgcg agggcggcta tgaacttgtt
     2161 gtaccggcgt ttttggaact gggtacgcct gaccttgcga cagccatcgg catcctggta
     2221 aaacaagggg caaatcgcat cgccgtaatc ccgtatttcc ttacgctcgg gttgcatgtc
     2281 gacgaggacc tgccgcgaat cgtggacggg atttcccgca tctataacgg tgtcaagatc
     2341 catgtgactg caccgctcga cgggcatccc gcgcttgttc gcgtacttct cgatcgtgcg
     2401 cgggaggcgg tggagagctc gcgtggcggc gaatgaggta attgaatttg ggaggtttac
     2461 gctgagagcg agactgatcg agtaccggga ccttgcgtgc gacgtacggc acttcgtgtt
     2521 tgaggtgcct gaaacggaac gcctgacgtt tgcccccggg cagtttctct cgttcatgga
     2581 gcaactcgaa ggcaagatgg tgacgcgtgc ttactcgatc gcgtctcctc ccgacggcaa
     2641 ccgtttcgat ctctgcctga accgggtgaa ggaaggtctg ttttcgccgc acctgtttca
     2701 tctggaaccg ggcgatgagg tggaggccaa agggccgtat ggcactttcg ctttgcggca
     2761 gccggcgagc gactccgtga tgatcgccac cggcaccggt atcgctccgt ttcgctccat
     2821 tctcctgtcg caggtgcctt ccgatacgga gcaccaatat acactcctgt ttggcgtccg
     2881 ttacgaggaa acattactct accgtgaaga gttcgaggca ctcggcaata ctcacccgaa
     2941 tttccgtttc tggcccacct tgacccgtcc ggagccaaaa tggacaggac gtgtgggacg
     3001 ggtgcaacag catctgctgg aagccctgga agagcggcgc gatgtggacg tttatatttg
     3061 cggcctgaag gagatggtga acgatatccg cggccggctc aaggagctgg gcttcgagcg
     3121 gcagcgggtt atttacgaaa agtacgactg aatcgtacaa tcgattcgtg ccttcgggtt
     3181 ccgccagaat cttcgcgatg gccgtgctgg ccatggttgt gtttgcggct acaacaggcc
     3241 ttctgatgtt cgcgttgccg gcgccgctca gctccacgga ttacctggtt attggctcgg
     3301 ccgcaacctt ggtttcactc agtaccgtgt tcgctgtggc tgctctctcc ggccgctcgc
     3361 tgcaaaaaga acgcaagata cgctaattcc caccacctaa cgcttttctt gcattttgtt
     3421 cgccaacaaa ccgaatggtg cagcgaaacc aagtagcact atctcctgtc gaaaaagctg
     3481 gacatgaaac tgtcattaga ttggacaggg cgcacattgt ccgctagtga gtgtttctgc
     3541 accctgtcat caacaatccc aaaaaggaga tcccggagaa gcatgcagtt taagctactt
     3601 ccaataattt tggccgcgat cgcggggccg gtgacagcgc agaatgcggg gagcaatccc
     3661 cctgccatcg atttcacgcc aaaccagggc cagtggagcc cgcaatttac gatagcgggc
     3721 ttcaccaggc cggatggggc cgccccggtt gtgtacgact ttgctcgcgg gagggatggt
     3781 gaagtcgttg cgaccggaat gttccggtgg gctggtacgc ggcgtgccgg tccggtagcg
     3841 cgtttggaag gagacgaatg gaggtccgta agggatgctt ggccccgcga gggggaagtt
     3901 gccttttcgg cgatcgcgat caacaacgaa ggggtgaccg ctctcagcac atatggcggc
     3961 ccctttggct cggttcctgt tgagatctgg ctggatactg gcgaggaagt tcgcgtcatc
     4021 ggaaacctgc aaggagcggt acgctctctg gcgtggttcg acggaaaact gtgggccgcc
     4081 ggcttctttc aaatggggca agggggcgtg agcaacctgg ccgtctggga cggaaatacc
     4141 tggtctgcgc cgcccggtgg acctgccgac cgggccgtgt atcggctagt gagcggcaat
     4201 tcgctgctgc tggcgggtga tttcactacg gtgggaggta ttcccgcccg gggcattgcc
     4261 gagtggaacg gcaacacatg gaaagcttac gatctcaatc tgccgatcgc agccgcccac
     4321 gtgtatgcgg tcgcgcgtgg tcaaagcggc gagctttatg caggaggcgc ggtaacggga
     4381 ggagtcctgc gctggaatgg aaccggttgg gagcagctcg gtggcggcgt gttccagcgc
     4441 gatacgaacg gcgtgatcag cgatttgacg acgcaccagg gcgaactcta cgtcaccggg
     4501 tgttttacga gtttgaacgg ccctgagtcc gatccatccg ccattcgcgc acagtctgtc
     4561 gccaaatgga gcggttcaca gtgggagagc ctcgacgatg gctcagacct gcttacccac
     4621 ggatggttcg agtggggtgt gtgtggagac gagccaaatc aatttacgat ttgggacgta
     4681 cggtatcaac ggctcttcag cgacggagaa cgcgtctttc tcggcggcat gatgccggga
     4741 gtagcgggca ttccttccca aagcatcgtg tcgtatgacg gaaacgagtg gagaggatat
     4801 ggagaaacgc aggatggtct gttcggcgcc agccagaccg tgatcgccgg cggtcccggc
     4861 cggaacgttt atatgacagg cttcgtctct catgccggac aggcacaatc gcgagcgtct
     4921 gtatatcgtt accagaatgg gtgggaggct gtggcacccc cttggccgca aggattgatt
     4981 tgtaaccgca tagctgtcga cccatggggg cggctgttcg ccggttgcta tgaatcaaac
     5041 caatccggtg agaatcagca gccgattgtg ttggggctta atggtgggga atgggcgccg
     5101 ctgggagtgc tggagttgcc aggccagatt tgggacatgg gcgtcgacca ttggggccgg
     5161 ctctgggtgg taggaggagt gcgcctcggc cccgcggagg gctctggttt tgttgcccgc
     5221 tgggatggag accagttcac gattgtggaa gaccgcttca attccctggt gttccagatc
     5281 gattttgcgc ccgaagaagc gggctcaccc ggtgacgtga tggtagcagg cgccttcacg
     5341 cgaatcggtg cagagagctt cgagcgaatc gcgcaatggc gcggcggacg ctgggagccg
     5401 ctaggggccg ggcttggtag cgctgtactg gcgctggcct ggggccggga tggggtctat
     5461 atctcgatgg aagctcccag ctcaagcgag gagccggaca cgccacggtt tctgctgggg
     5521 cactgggacg gctctcgatg ggttgagttg gcaactccgg agaacggctt gcctgcgcca
     5581 cgcgagaaca ccgttcatca atttcgagat ctgcttgcgg ttggcagata cgtgattgcc
     5641 gcgggatcgg tttggcccga gaccgggggc cggaatgtat ttgtttatga cggtcaacgg
     5701 ctggcacctt tgggaggcgg attgaacgcg ataggcgtct cttccatcgc gctcgccaat
     5761 gacggtctct ggttcgctgg cgaaatcgcc gaggcgggct ccggcgatca ggtcattccg
     5821 tccgtcggta ttgcccggtt tacagctccg gtggaatccg aaccttgatg attggggacc
     5881 gcgccgggcg acgcgtcgtt cggtgcggtc cctcacgatt cagagcggtc gaatcttgat
     5941 gttgcggaac cagacttcat cgacgtgatg ctgtaaggcg atcgaggttt gtttcttcgg
     6001 ctgctttgta agaagctcgt agaccggcga ccgggtggtc cagcgctcgg cgagttgttt
     6061 tgcgatttcg ggcgagtcga gtgaggtgtc cactattttc gctccgttta accaatgttg
     6121 tacgtggttt ccccgcagga taatgcgcga ctcgttgaat tcgccagccg ggcggaccac
     6181 aggcttgact ggcgcaacca gtgagtaaat cgaaccggca gaacggtcgg cattgcggcc
     6241 cgcatcggga tgcttgtcgt tatcgatcag ttgatattcg aaagcgatgg cgtactcttc
     6301 gtagtttgct cccggcggaa tagtgcttct gctggaagcg cgatgaagta gctcataatc
     6361 caccgtatct tcaaatctcc tggcatctgg gttcagcttg ccttttatga gagacacccg
     6421 atcctgaatc cggtacttga cgccgctatt gcctccggaa gaaatcttcc aatcgaacgc
     6481 caactcgaaa tcgccgaatt gatcgatggt gaacaagtcc tcgcgggtgc gtggtttctc
     6541 caccgctttg atacacccgt ctttgaccac ccaagcgtca ccggcgggag acttggctgc
     6601 gggatcctcc cacccggcca gagtcttgcc gtcgaagagc agccgccatc cggcggaccg
     6661 ttcctcggaa gttaattggt tatggtggac ctgagaagac ggactggagc agttcatcag
     6721 ggacaaagcg caaacgaaca gtagcgggaa caaacgggcg ttcatagtat cagggtaaag
     6781 taaacctcat gtgaagtgcc ctatactggc gaggggcctt gaccggggag gcagcgcatg
     6841 agtcgaattc taatctttgt gttggttgcc gcagcggcag cagagacgca cgagatctat
     6901 ccggagacgc actcgcgtgt gttttcggca ttcaaggaac cggtgctgcg agtgaaatcg
     6961 ggtgacacgg tgatcacgcg aacctgggat tcaggcgggc aggattacaa aggcgttcgt
     7021 cacatccagc atccgtatgt ctatccggag agtggaaatc ccttgatggg tcctttctat
     7081 atcgaagagg ccgagtacgg ggatgcgctc gaagtacacc tggataaggt gcggctgaac
     7141 aggaattggg gatacacgtc gtaccggctc atcccggggg tgttgagcaa cgtcgaagac
     7201 ctatacagga atttctacaa aatggacgct ctgcggcccg ggcgcgccga cctgatcccg
     7261 tgggatatag acctggagcg tggcgaggca agcccgcgtc tgctcgaagg acggggattc
     7321 aaagtgcagg tacgtccgat gctgggttgc atcggagtcg ccccgcctgg tgaggaagtg
     7381 cagacctcgg gtccctcttc gacgcacggg ggcaacatcg actacaacga tatcgtcgaa
     7441 ggagtgacgg tgtattttcc ggtgtaccac aaaggtgctt tcttctatgt gggagatggt
     7501 catgcggtgc aaggcgatgg ggagggtctg ggaaccggtg tggaaacatc gctcgacgtg
     7561 cagttcaccg tgagcgtaca caaaggaaaa cggctcagta taccgcgcct cgtgaacaag
     7621 gaatatatcg tctcgatcgg cagtcagccg gagttccgca gcagtatgga tttcggtctc
     7681 cggatggcaa attcggacat gatccgctgg ctaacaagcg agtacaagct taccgtcccg
     7741 gaggcacatc tgctgatggg tactgtggtg cagcacaaga ttgtgacgta ttttggcact
     7801 atcgcgacgc tgatcccacg aaagtacctt ccgagccggt agctggactt gggcgagttg
     7861 ccggtctggt ctgaaccgtc ttacgctaaa ggcaggtctt tccctccgca tgatttcggt
     7921 aaagctcccc accgtttcgt tatgctgcat cctggcgctg gtcctggttc aagaggtatc
     7981 cccggatgca agaacaactc cgcccgtcca cgtttccccg gcgttggcag ccgcactgga
     8041 gcagattacg ccggaccggc tgaaaggcga cgtatccttt cttgcttccg atgcgctgga
     8101 aggccgcgcg acgccgtccc gagggctgga ggttgccgcc gaatatatcg ccgcgagatt
     8161 ccgggccgcc ggtttggaac ct
//
LOCUS       DASTMK010000101         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_420689, whole genome shotgun sequence.
ACCESSION   DASTMK010000101 DASTMK010000000
VERSION     DASTMK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420689"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VFQ79_04040"
     CDS             <1..830
                     /locus_tag="VFQ79_04040"
                     /inference="COORDINATES: protein motif:HMM:NF023598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HEU0138851.1"
                     /translation="VPGILFALGVLAPRDMAWLWEKIVDFCKGTRAVPGGDSACGFAN
                     TAMQLAHQKMLPEVLAAVVRAASAVRSLTAFEQGAVGPSKDCAYEGPILKAITGRPIS
                     MEGKSATCAHFSSVGNIAGAVCDLWSNVSVQNIRLLSGNAPEAYTELLAYDCRLMNTA
                     ARNGEARILRDWFVKSDEWLSPQAAVLSPRAALRIARRIMHEQDPYRRTVAAAAEAVA
                     ILREGTDAGRWTPSKKENQWLQKIEDAIAVMPADDAGMLREARETYGALFQDDSYGL"
     gene            914..2056
                     /locus_tag="VFQ79_04045"
     CDS             914..2056
                     /locus_tag="VFQ79_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU0138852.1"
                     /translation="MKFKTLAIHSGHRNEEHYGAVMTPVYQTSTFAFRGVNEPGPFDY
                     SRSGNPTRKALEECLAALEGGSAGFAFATGMAAETTALMLFNSGDTIVVHNDLYGGTY
                     RLFESVFRSKGLRACYVDLRDTDTLERTLRDGAAAVWIESPTNPLMNLVDLRATADAA
                     HRHGALAICDNTFLSPYFQRPLEFGVDLVVHSTTKYINGHSDVVGGAIIVKDAGLAER
                     VGFLQNAMGTCAGPQDCFLVLRGIKTLALRMEEHNRNALAIAEWLSHHPKVERVLHPG
                     LPSHPQHQLALTQMRGYGGTFSFHIRGGQEAAFRALSGVRVFTLAESLGGVESLIEHP
                     ATMTHVSMPPDVREAMGITPSLIRVSVGLEDVADLIEDLNTALESA"
     gene            complement(2070..4658)
                     /locus_tag="VFQ79_04050"
     CDS             complement(2070..4658)
                     /locus_tag="VFQ79_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138853.1"
                     /translation="MRTLRRWLMRARGLFGRERIEREFAAELASHLELHIDEGVRAGL
                     SPGEARRQALVMLGGLAPVQELYREQRGLPVLENLVRDFVYSLRLLRKSPGFTAVAIL
                     SLALGIGANTAMFSLVNAVLLRALPYPESGRLLHTVRDVTLPEYEALKQQSAIFSDVA
                     GRQGPSEKRLDYGDRREWIAALRITPEFFRTLNVPLSMGGEFTSTTADRTVILSHDLW
                     RRLMQARPDAVGQVVALDGKAYMVAGVAAPHLWLPDQADAFIPLNATGGFGDLGANTQ
                     VISRLAPGVDRRQANSAAAAAAGIFRRAYPEYARQDQGIALVPFQEWLTGDVRGKLLL
                     LSGAVGLVLSIACFNLIGLLLARLSARQREISVRLALGCSRGRLLQQFLMENLLVCIA
                     GGVAGVLGAYWLLDFFLAILPFPLPSAAPIRVDLPVLAFAAGITLVAGLLVTLAPAWM
                     FSQPNIQGTLKQSARGVGERTGLRSILVVSEIAAATALLIGAALFLHSLYLLNRQPLG
                     FTPEGLLTFFAPPEPGAGAARNHSLISTLHEQFSGIPGVRRVAAVNVLPLAGKNNFPA
                     QRAGHPEQSIGGMEIRLVTPDYFETMSTPLRRGRCFTALDTEQTVPVVLINEALSRQW
                     WPHGNAIGDRIEVGTLRGKPVMREATREVIGVIGDMRILHLAEPARPTLYIPIAQARW
                     YDGGMNWVIRADAGKGFAEVVRRKVKEVAPGQRIERMRSMEQIIATTTANPRFDAVLF
                     GSFAASALLLAALGVYGLLAFSVAKRTSEIGIRMALGASPGQVLRLVLSQGLQLVAFG
                     LAMGIAGALILGRYLSNLLFGISPADPLSFAAVAAVLSASGFLASYLPARRAMRVDPL
                     QALRCE"
     gene            complement(4655..4999)
                     /locus_tag="VFQ79_04055"
     CDS             complement(4655..4999)
                     /locus_tag="VFQ79_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0138854.1"
                     /translation="MSRKSDVWQGTLALMVLKTLETMGPLHGYGIARRIEATSGDRLS
                     VNYGTIYPALLKLEQEGAIASEWGVSENNRKAKYYKLTRAGRKLAEREAREWEETAAT
                     IARFLNPAKEPL"
BASE COUNT         1094 a         1596 c         1491 g          965 t
ORIGIN      
        1 aggtgccggg catccttttt gcgctgggcg tgttggcgcc gcgggatatg gcgtggctct
       61 gggaaaaaat cgttgatttc tgcaagggca cacgagccgt gcccggcgga gattcagcct
      121 gcgggtttgc aaataccgcg atgcaactcg cgcatcaaaa aatgctgccc gaggttcttg
      181 ccgcggtggt ccgcgctgcg tccgccgttc gaagcctgac cgctttcgaa cagggagccg
      241 ttggcccctc caaggactgc gcgtatgaag gtccgatcct caaagccatt acgggccgcc
      301 ccatctccat ggaaggcaaa tccgccacgt gcgcccactt cagttccgtc gggaacatag
      361 cgggagcggt gtgcgacctc tggagcaacg tgtcggtcca gaacattcgt ttgctatccg
      421 gaaacgcacc ggaggcgtac accgagctgc tggcatacga ctgccggctg atgaacacag
      481 cggctcgaaa cggcgaagcg aggatcctgc gcgattggtt cgtcaaatcc gacgaatggt
      541 tgagcccgca ggccgcggtc ttgtctccaa gggctgcttt acgcattgcg cgccggatca
      601 tgcacgagca ggacccttac cggcgcacgg ttgcggcggc agcggaagcc gtagcgatcc
      661 tgcgcgaagg aactgatgcc ggacggtgga cgccctcgaa gaaagaaaat cagtggctgc
      721 aaaagattga ggatgcaatc gccgtcatgc cggccgatga tgccggcatg cttcgagaag
      781 cccgcgaaac atatggtgct ttatttcagg acgacagcta cggtttgtag gactgcacgg
      841 cgtcaaccca cagcccgcaa gagttatctg agttccgtgc tgtttgaagg ttgggcctga
      901 gacaatcaat taaatgaaat tcaagacctt ggcgatccat tccggccacc ggaatgaaga
      961 gcattatggc gctgtcatga cgcccgtcta ccaaacctct acgtttgcct tccgtggcgt
     1021 caatgaacct ggcccgttcg actactcccg cagcggaaac cccacccgta aagccctcga
     1081 ggaatgcctt gcggcgctgg aaggaggcag cgcaggtttc gcgttcgcca caggtatggc
     1141 agcggaaaca accgccctga tgctgtttaa ttccggcgac acgattgtag tccacaacga
     1201 cctgtatggc ggcacctacc ggcttttcga gtcggtgttc cgcagcaaag gccttcgcgc
     1261 ctgctacgtc gacttacgag atacggacac gctggaacga acgctgcgcg acggcgcggc
     1321 cgcggtctgg atcgaatccc ccacgaatcc gttgatgaac ctggttgatc tccgggccac
     1381 cgccgatgcc gcgcaccggc acggcgcgct cgccatttgc gacaatacgt ttctctcgcc
     1441 gtatttccag cgaccgctcg aatttggcgt cgacctcgtt gttcattcca caacgaagta
     1501 catcaacggc cattccgacg tagtgggcgg cgcaatcatc gtcaaagatg ccggactggc
     1561 ggagcgtgtc ggatttctgc aaaacgcgat ggggacgtgt gccggtccac aggattgctt
     1621 tctcgtgctg cgaggcatca agacattggc tcttcgcatg gaagaacaca atcgaaatgc
     1681 tcttgccatt gccgaatggc tgagccatca tccgaaagtg gagcgtgtgc ttcatcccgg
     1741 acttccatcg catccgcagc accagcttgc gctcactcag atgcgaggct acggaggaac
     1801 cttctccttt catattcgcg gaggacagga ggcagcgttc cgcgcgcttt ccggtgttcg
     1861 agtattcaca ctcgccgaat cgttaggcgg tgtcgagtcg ctgatcgaac accccgcgac
     1921 catgacgcat gtgtcgatgc cgcctgatgt ccgggaagcg atgggcatca ccccaagtct
     1981 catccgcgtt tcggtaggcc ttgaggatgt cgccgacctc atcgaagact tgaacaccgc
     2041 cctcgaaagc gcctgatgaa cgcgcgctgt tactcgcagc ggagtgcctg caacggatct
     2101 acacgcatcg cccggcgtgc gggcaagtag ctcgccagga aaccggatgc cgatagcaca
     2161 gcggcgacgg cagcgaagct gagagggtcc gccggtgaga taccaaagag aaggttagag
     2221 aggtaccggc ccagtatcag cgcgccagcg attcccatcg ccaaaccaaa tgcgaccagt
     2281 tgcagacctt ggctcagcac cagccgcaat acctgtccag gcgaagcgcc caatgccatc
     2341 cgtattccga tttcgcttgt acgcttcgcc acggagaacg ccagcaagcc gtagaccccg
     2401 agcgctgcga gaagcaaggc agaagccgca aagcttccaa aaagcaccgc gtcaaagcgt
     2461 ggattcgccg tggtggtagc gataatctgc tccatgcttc gcatacgctc gatgcgctgc
     2521 ccgggagcaa cttccttcac cttgcgccgg actacttcgg cgaacccttt ccccgcatcc
     2581 gcacggatga cccagttcat cccgccgtcg taccagcggg cttgagcgat gggaatatac
     2641 aaagtcggcc gcgccggttc ggcgagatgc aagattctca tgtcgccaat cacgccgatg
     2701 acttcgcgcg ttgcctcacg catgaccggc tttccacgca aggttcccac ctcgatgcga
     2761 tccccaatcg cgttcccgtg cggccaccac tgccgtgaca gcgcctcgtt gatcaggacg
     2821 acaggcactg tctgctctgt gtcgagcgcc gtaaagcagc ggccgcggcg caacggcgtg
     2881 ctcatggtct cgaagtaatc cggcgtgacg agacgtatct ccatgccgcc gatactctgt
     2941 tccgggtggc cggcacgctg cgcgggaaag ttattcttgc cggcgagcgg cagaacgttt
     3001 accgccgcca cgcggcgcac gccgggtatc ccggagaatt gttcgtgaag cgtggagatc
     3061 agagaatgat tccgcgcggc gcccgcaccg ggctcgggcg gagcgaaaaa ggtgagcagc
     3121 ccttccggcg tgaaacccag aggctgccgg ttgaggaggt acaaactatg caggaacagc
     3181 gcggcgccga tcaatagggc tgtggcggcg gcgatctcgg agacaactaa tatgctccgc
     3241 aatcctgtgc gctcgccgac tccccgcgcg gattgtttca aagtgccctg aatattcggc
     3301 tgcgaaaaca tccaggcggg cgccagggta accagcaatc ccgctaccag cgtaatcccg
     3361 gctgcaaacg cgaggaccgg taggtctaca cgaatcgggg cagcagaggg caaggggaat
     3421 ggcaggatcg caaggaagaa gtctaataac caataagcgc ccaacactcc cgctacacct
     3481 cccgcgatac agacgagcag gttttccatc aggaactgct gcaacaggcg gccgcggctg
     3541 catcccagcg ccagacgcac ggagatttct ctttggcggg cagacagacg agccaacagc
     3601 agacctatca aattgaagca tgcgatcgat agcaccaatc ccaccgcgcc tgataaaagc
     3661 aggagtttgc cacgcacatc gccggtgagc cactcctgga aagggaccag cgcgatgccc
     3721 tgatcctggc gcgcatattc cggatacgca cggcggaaaa tcccggcagc tgcggcggct
     3781 gcggagtttg cctgacggcg atccacgcca ggtgccaggc gggagatcac ttgtgtattg
     3841 gcccccagat ctccgaatcc ccccgtcgcg ttcaacggga taaaggcatc agcctggtcc
     3901 ggcagccaaa gatgcggggc cgccactccg gccaccatgt acgccttccc gtccagcgca
     3961 accacctggc caactgcgtc cggacgcgcc tgcataagac gccgccaaag atcgtgactc
     4021 aggatgacag tacggtccgc cgtggtggag gtgaattccc cgcccatgga tagcgggacg
     4081 ttcaatgtac ggaagaattc aggtgtgatc cttaaagcag cgatccattc ccggcggtcc
     4141 ccgtaatcga gacgcttttc cgacggaccc tgccggccgg caacatcact gaaaattgcg
     4201 gattgctgct ttagggcttc gtattcgggt agcgtgacat cccgcaccgt gtgcagcagg
     4261 cggccagact ccggatacgg cagtgcgcgc agcaggacgg cattcaccag gctgaacatc
     4321 gccgtgttgg cgccaatgcc cagagccaga gaaagtatcg ccaccgccgt gaagccgggg
     4381 ctcttccgca agagccgcag tgagtagacg aaatcacgga ccagattttc gagtaccggc
     4441 aagccgcgct gctcgcgata cagttcctgt accggcgcaa gcccacccaa catcaccagt
     4501 gcctgacgtc tggcttcgcc gggagataac cccgccctca caccttcgtc aatatgcagt
     4561 tcgagatgcg aggcaagctc ggcggcgaac tcccgttcaa tccgctctcg cccgaacaaa
     4621 cctcgtgcgc gcatcaacca gcggcgcaac gttctcatag tggctccttc gcgggattca
     4681 gaaagcgggc gatggtagcg gccgtctcct cccactcgcg cgcttcgcgc tctgccagct
     4741 tgcgcccggc acgcgtcagc ttgtagtact tcgcctttcg attgttttcc gaaacacccc
     4801 actcggaagc gatggcgccc tcctgttcaa gcttgagcag ggccggatag atcgtgccgt
     4861 aattcaccga taatctgtcg ccgctggtag cttcgattcg acgggcaatg ccatagccgt
     4921 gcagcggtcc catcgtttcc agagtcttca gcaccatcag ggcaagagtg ccctgccaga
     4981 catcgctttt cctgctcatg gtctttccta ttgctaacca atactactgc ttgccgtatt
     5041 ggaatgcaat aggaaaaaag actgcggaaa cgctcctccc cccttaaggg agcgcaagga
     5101 gctaccattc ggataaatgt caaggtgggt ttttgccgtt ctcgcc
//
LOCUS       DASTMK010000102         8118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_165007, whole genome shotgun sequence.
ACCESSION   DASTMK010000102 DASTMK010000000
VERSION     DASTMK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165007"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /locus_tag="VFQ79_04060"
     CDS             complement(<1..2231)
                     /locus_tag="VFQ79_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEU0138855.1"
                     /translation="MPLPLPNLDDRRWAGLVEEGRGLIPRHAPAWTDHNLHDPGITLI
                     DLFAWLAEMACYRLNRVPERHRRKFLALIGFTPRDPVPSYGMLQFTPASGSAGFVLPA
                     GTEFEGTAPDQRRMPFRTLRDMTVSPVELHALLVDAGDGTLWDRSRDLADGLPIYPFG
                     SDPQSGAALYLGFDEISVGTPVALGFRFLGPGNDLAERLRLIEESVAHQLSCRSVQPR
                     FTCGKSTPEPSDALPAHHSARVVWEVFASGGWTELTPQDGTRSMTLDGIVEITLPASA
                     ERTALASVAAPLFYVRARLAAGQWDAAPALLDVAPNTALVEQAVPVTETFVIDAAVTP
                     TGTAPVPGTLTRLSLEVDERKIIQTLAFGAATGPELRVWSYTAPSGGMAGELTVEAEM
                     AGWGTGRPDQTLAPRNAPADSRTLRLYTLASGMWQEWTRRADFDSSARTDFHYTIDAT
                     SGALSFGSGERGRVPEAGSLVFATYRSTNADQGNLGRGTITSLRRSPVNVVLLSALSA
                     ATLDQLDQITTNHAGASGGLAAETLNRTAGRAVETLHAHQRILDLAAEARSTTLDQVD
                     RVRVRALQPPSRGVNLLDLERIALSVPGTRIARARAWAATHPDYPCLTAPGVVTVIVV
                     PELPVEAPMPSEGLLEAVRSYLEPRRMLCTVLKVTGPEYLDVNVSAQVRIRANASAER
                     VRVRILEALDEFLHPLRGGPERLGWPFGRDVYRSEILQLMDEVAGVDHVLSLSITAGG
                     DQGS"
     gene            complement(2231..5227)
                     /locus_tag="VFQ79_04065"
     CDS             complement(2231..5227)
                     /locus_tag="VFQ79_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEU0138856.1"
                     /translation="MTDSSTPVLDPRTSAGIVDELFELRPAWTPELVPLPGDPAWALF
                     QIFGRYAQIVLDRLNQAPDKNLLAFLDMLGISLVPAKPARAPVVFLPVPNAADAIVPA
                     GTRLGAKVPGRSDPLIYETEETQAIAAARLTDLFTLWPARDEYTDHSPQAVTAPEPFT
                     LFQPPKPVPHEFYLAHDTLFAFSGRATLEIEFELSAPGSKPMTIGWEFWDGNVWRPFR
                     EFDPANPDAGRDGTNGFTRSGIVSLKAECGESKKTKVNEVEAHWIRGRATEPLPTEPG
                     RAWPMVDRIRVRSVILAEEMSAFAPETAFADALKLDIGKAFYPFDQQPKPGSALYLNS
                     EPAFAKPGARVTLDADDSATPLESNYTITQNPTLAMEYWNGRSWQNLDIATSTLLAFA
                     KSGTSFQFTVPADIAKGKVNDQEGYWVRIGIVAKTFAAEQEVKFTDSSSSTTNTLKLV
                     TTIPPALQNLRIAYTYRSPWHRPDHCLTFHDLQWTVHSDDIRWPGNPFPAFRDIADTT
                     AAVYFGFDRPLPNDTVGIFLGIIENPKTAPRLVWEAWNGTAWVEVTVEDSTGGFSRPG
                     LVRAIAPALSVPAETALLARFGTPRVWLRTRLKEDGAPLESTVGGVWFNAAWASQLET
                     VAGEVLGGSRGVPNQTFFFTRTSILPGEEIEVRELEGARAHVEFPILQDELLSRGFTT
                     DDLRTVVDPRTGKVREVWVRWRGTPHFYFSGPDDRHYLIERARGRLIFGDGLSAKIPP
                     AGTGNIRAKLYRAGGGSLGNVPAGAISQIMSGVLAQGVSNPRSAEGGADGEVPEAAAV
                     RGPNVLRHRERSLSAADYEALAKEASPAVAAARLLPATHANGRPAPGWVTLAIVPHSA
                     DAQPQPSYELRQQVHAYIAARAPATLPAEHISVIGPKYLAIGISALLIPRDLSDAGRA
                     QQGAVIALARFLHPLTGGPEGRGWPFGRGVYQSDVAAILENIPGVDHVEQLQLRLDDI
                     PAGDSVEVAPDRMVVAGPIRIEVRGI"
     gene            complement(5224..5643)
                     /locus_tag="VFQ79_04070"
     CDS             complement(5224..5643)
                     /locus_tag="VFQ79_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEU0138857.1"
                     /translation="MAAEKLDPNKAFLGVGLAFPPQADVHGNLATVAYEEDIRQAIRV
                     ILGTNPGERVMRPDFGAGLRQFVFEPADATIVHRVETRVREALIDFEPRIDVVELRVT
                     VDSPLRRTLLIHITYRVRATNTLANLVYPFYLEEAKR"
     gene            complement(5645..5926)
                     /locus_tag="VFQ79_04075"
     CDS             complement(5645..5926)
                     /locus_tag="VFQ79_04075"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEU0138858.1"
                     /translation="MPPAARVTDPTGHPGMISGPGVPNVLIGGLPAAVLGDLHACAMP
                     PTAGPHPPNPIAAGSATVLIGGRPAARMGDITGCGAPIVMGLPTVLIGG"
     gene            complement(5937..6599)
                     /locus_tag="VFQ79_04080"
     CDS             complement(5937..6599)
                     /locus_tag="VFQ79_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEU0138859.1"
                     /translation="MIETVLDRIDSSIERLNKKHYGVVTGRVINILDPLMLGRVQVQL
                     PFSDSVDLSPWARIAVPMAGPLHGTYMIPNIGDEVLVAFEHGDMRVPYIIGSLWNAMS
                     PPPLPSPAPQIRLIKTLVGNQIMITEAPPSITIQVMATGQTILIAPTGIQILSGANVI
                     NLGPPTGPPTAQVVSGSNAINMSPDGITISGTPNLNLTASGVLNITAPAVSITGGLVK
                     IN"
     gene            complement(6596..7780)
                     /locus_tag="VFQ79_04085"
     CDS             complement(6596..7780)
                     /locus_tag="VFQ79_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138860.1"
                     /translation="MPEYATYAPEFRIQINGQDMPAQVRSAVTSVRYEDGRQAADRVE
                     LGIANPNLRLLQNHIRGLGFRPFPSAISLGPVKLGQAAPEGTFDLDNKLTLAVGYAAG
                     QLENMFEGDITGVSVTFPNGGMPTMQLVAHDYLNRLQRGKYSRGFGFLPDAIIASIMS
                     AENLLIPAIDPTIVAGSTALAVVNYVFGGASRRQRGQSDFELLEEIAATYDADFWVDG
                     NVLYLSRFLKEYTPRLTLTYGKNLFDFSPRVSTIGQVAGVAMRFVLREIPLDFLVAIG
                     YDFDRESIVISVVPGAAASATQSLLGSLLTLIDQPIGSPADITNSALIIASELRNKLN
                     NRLTGSGSCIGDPRIRAGALIGIEGLGPDFSGNYRVSSATHTIDGSGYRTSFEVKKEI
                     IP"
     gene            complement(7780..>8118)
                     /locus_tag="VFQ79_04090"
     CDS             complement(7780..>8118)
                     /locus_tag="VFQ79_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU0138861.1"
                     /translation="CVLARASQKFILFFPDGRPARVRINVTFNEFIDADREAKEINRQ
                     TADFVKLHIVKQGETLSGIAASLYDNPLLWRPIAIVNGIENPRALITGQSLMVPPLPF
                     IDPESGEVLE"
BASE COUNT         1510 a         2518 c         2607 g         1483 t
ORIGIN      
        1 gagccctgat ctccgccggc ggttatagac agcgaaagaa catgatccac acccgcgact
       61 tcatccatga gctggagaat ctcgcttcga tatacgtcgc ggccgaaggg ccatccgagg
      121 cgctcaggac ctccccgcaa aggatgcaga aattcgtcga gcgcttccag gattcgcact
      181 ctcacccgtt cggcgctggc attcgcccgg atgcgaacct gcgccgacac attcacgtcc
      241 agatattcgg ggccggtgac cttcaggacg gtacacagca ttcgccttgg ctccagataa
      301 cttcgaaccg cttccagcaa accctcggaa ggcatgggtg cctccacggg caattccgga
      361 acaacgatca cggtcacaac gccaggcgcg gtcaggcaag ggtaatccgg atgcgttgcg
      421 gcccatgcgc gcgctcgcgc gatacgagta cccggcaccg acagggcaat gcgctccagg
      481 tcgagcaggt tgacgcctcg tgacggaggc tggagcgcac gcacacgcac gcgatcgacc
      541 tggtcgagcg tcgtgctgcg ggcttcggcg gcaagatcca ggatgcgctg atgcgcgtgc
      601 agcgtctcga cagccctgcc cgctgttcga ttcaacgttt cggcggcgag gccgccgctg
      661 gcaccggcgt ggttggtggt gatctggtcc agttgatcga gagtagcggc ggatagcgcg
      721 ctcagcaata cgacgttcac gggcgagcgt cgaagcgagg taatcgtacc ccttcccagg
      781 ttgccttgat cggcatttgt cgaacgatag gtcgcgaaaa ccagtgagcc agcttccggt
      841 acacggccgc gctcgccaga cccgaaggac agcgctcccg atgtggcgtc gatcgtgtaa
      901 tggaaatcgg ttcgcgcgga cgaatcgaaa tccgcgcggc gagtccactc ctgccacatt
      961 ccactggcca atgtatagag ccgcagcgtt cgcgaatccg cgggcgcatt gcgcggggcg
     1021 agagtctgat ccgggcgccc cgtgccccaa ccggccattt ccgcttcgac cgtcagctca
     1081 cccgccatgc ctccggacgg cgccgtgtag ctccacacac gcagttcggg tcccgtagcc
     1141 gcgccgaagg cgagcgtttg gatgatcttc cgctcgtcca cttcgagcga caggcgggtg
     1201 agcgtgcctg gaaccggagc cgttccggtg ggggtgaccg ccgcatcgat cacgaacgtt
     1261 tcggtaacag ggacggcctg ttcgactagc gcggtattcg gcgctacatc gagcagcgcc
     1321 ggcgccgcgt cccactgccc tgcggccagg cgcgcgcgca catagaacag cggcgcggcg
     1381 acagacgcca gcgcggttcg ttccgccgat gcgggcaaag tgatttcgac aatgccgtcg
     1441 agcgtcatgg aacgcgtgcc gtcttgcggt gtgagttccg tccagccgcc gctggcgaac
     1501 acctcccaaa caacgcgcgc cgaatgatgt gccggcagtg catccgatgg ctccggagtg
     1561 ctctttccgc aggtgaaacg cggttgcacg gagcggcacg acagttggtg agcgacggat
     1621 tcttcgatca gccgcaggcg ttcagcgaga tcattgcccg gtccgaggaa tcgaaatccg
     1681 agggccaccg gcgttcccac cgaaatttca tcgaacccca ggtagagtgc tgctcccgat
     1741 tgtggatcgc tgccgaacgg atagattggc agcccgtcgg cgagatcgcg cgaacggtcc
     1801 caaagcgttc cgtcgcccgc atccaccagc agcgcgtgca actctacagg agatacagtc
     1861 atgtcacgca gcgtgcggaa cggcattctg cgctgatcgg gcgcggttcc ttcgaattcg
     1921 gttccggcgg gcaaaacgaa accggcgctg ccgcttgcgg gcgtgaattg gagcatgccg
     1981 taggacggca cggggtcacg cggcgtaaat ccgatgagcg cgagaaactt tctccggtgc
     2041 cgctcgggaa cgcggttcag gcggtagcac gccatctccg ccagccacgc aaacaggtcg
     2101 atcagcgtga tgcccggatc gtgcaggttg tgatcggtcc acgccggtgc atggcgcgga
     2161 atgagcccgc ggccctcttc caccaggccg gcccagcggc gatcgtcgag attgggcaaa
     2221 ggcagcggca tcagattcct cgcacctcga tgcggatcgg gccggccacc accatgcggt
     2281 ccggcgccac ctcgacgcta tcgccggcag ggatgtcatc gaggcgcagt tgcagttgct
     2341 cgacgtgatc gacgccgggt atgttctcca aaatggcggc cacgtcggac tggtatacgc
     2401 cgcggccgaa cggccatccg cggccttcgg gaccgcccgt gagtggatgc agaaaacgcg
     2461 ccagcgcgat aacggcgccc tgctgcgccc ttccggcatc actcaaatcg cgcgggatga
     2521 gaagcgccga aatccctatg gcgagatact tcgggccgat gacggagata tgttcggcgg
     2581 gaagcgtcgc cggcgcgcgc gcggcaatat aggcgtgcac ctgctggcgg agttcgtagg
     2641 agggctgcgg ctgcgcgtcg gcgctgtgcg gaacaatggc cagcgtcacc catccgggcg
     2701 cgggccgccc gtttgcgtga gttgccggca acaggcgggc ggcggcgacc gcgggagaag
     2761 cctctttcgc cagcgcttca taatcggccg ccgacaggct gcgttcgcga tggcgcagca
     2821 cattgggccc gcgcacggcg gcggcttcgg gcacctcgcc gtccgcgccg ccttcggcgc
     2881 ttcgcggatt cgatacgccc tgcgccagca cgccgctcat gatttgcgat atcgcaccgg
     2941 caggcacatt cccgagcgat cctccgccgg cgcgatacag cttcgcgcga atattgccgg
     3001 taccggcggg aggaattttc gcgctgagcc cgtcgccgaa gatcagccgc ccgcgggcgc
     3061 gctcgatgag atagtgcctg tcgtcgggac cggagaaata gaagtgaggc gtgccgcgcc
     3121 accgcaccca gacttcccgg actttcccgg tgcgcgggtc gacgaccgtt cgaaggtcat
     3181 cggtagtgaa gccgcgcgac agcagttcgt cctgaaggat gggaaactcg acgtgggcgc
     3241 gtgcaccttc gagctcgcga acttcgatct cttccccagg aagaatagag gtgcgggtaa
     3301 agaagaacgt ctggttcgga acgccgcggc tgcccccgag cacttcgccc gctacggttt
     3361 caagctgcga ggcccacgcc gcgttgaacc agacaccgcc aacggtgctc tcgagcggtg
     3421 cgccgtcttc tttgagtctc gtccgcagcc agacccgcgg cgtaccgaat cgcgcgagca
     3481 gcgcggtttc cgccggcaca gacagagcgg gcgcaatcgc ccgcaccaac cccggccgcg
     3541 agaaaccgcc ggtgctgtct tccacggtga cttcgaccca agcggtcccg ttccatgcct
     3601 cccaaaccag gcgcggcgcg gtcttcgggt tctcgatgat gccgagaaaa atgccgacag
     3661 tatcattcgg aagcggccgg tcgaagccga agtaaaccgc cgcggtggta tcggcgatgt
     3721 cgcgaaaggc agggaacgga ttgcccggcc atcgaatgtc gtcgctgtgt accgtccatt
     3781 gcaaatcatg gaacgtgagg cagtgatccg gccggtgcca gggcgagcga taggtgtaag
     3841 cgatccgcaa attctgaagt gcgggcggaa tcgtcgtcac cagcttgagc gtatttgtcg
     3901 tgctggaaga cgagtctgta aacttcacct cctgctcggc ggcaaacgtc ttcgcaacga
     3961 ttccgatgcg cacccagtag ccttcctggt cattcacctt gcctttcgcg atgtctgccg
     4021 gaaccgtgaa ctggaaggac gttccgctct tggcgaatgc cagaagcgtg ctcgtggcga
     4081 tgtcgaggtt ctgccaggac ctgccgttcc agtactccat ggcgagcgtg gggttttgcg
     4141 tgatggtgta gttggattcg agcggcgtgg ccgagtcgtc ggcatccagc gtgactcgtg
     4201 ctccgggctt ggcgaatgca ggttcggaat tcaggtagag cgcggacccg ggtttcggtt
     4261 gctgatcgaa aggataaaag gccttgccga tatccagttt taaagcgtcg gcaaaggccg
     4321 tctccggcgc gaacgcgctc atctcttcgg cgagaatcac ggatcgcacg cggatgcggt
     4381 ccaccatggg ccaggcgcgg ccgggctcgg ttggcagcgg ctcggtagcg cggcctcgta
     4441 tccagtgcgc ttcaacctca tttactttgg tttttttcga ctcgccgcac tcggctttga
     4501 gcgagacgat gccgctgcgc gtgaatccgt tggtgccgtc gcgtccggcg tccgggttcg
     4561 cgggatcgaa ctcacggaac ggccgccata cgttgccgtc ccagaattcc catccaatcg
     4621 tcatgggctt gctgccgggc gccgacaatt cgaattcaat ttcaagcgtc gccctgcccg
     4681 aaaaagcgaa gagggtgtcg tgtgcgagat agaactcatg cggaaccggc ttcgggggct
     4741 gaaataacgt gaagggttcc ggggctgtta cagcctgagg cgagtgatcg gtatattcgt
     4801 cgcgcgcggg ccacagcgtg aacaggtccg tcagcctggc cgcggcaatc gcctgcgtct
     4861 cttcggtttc gtaaatcagc ggatcgctcc ggccgggcac cttcgcaccc aggcgcgtgc
     4921 ccgcgggtac aatcgcatcg gctgcgttcg gcacagggag aaagacaacc ggcgcgcgcg
     4981 ccggtttcgc cggaaccagg ctgatgccga gcatgtccag aaacgcgagc agattcttgt
     5041 ccggcgcctg gttcagccgg tcgagcacga tctgcgcata gcgcccgaag atctggaaca
     5101 gcgcccaggc aggatcgccg ggcaagggca ccagttcggg cgtccaggcg ggccgtagct
     5161 cgaaaagctc gtcgacaatg ccggcgctgg tgcgcgggtc gagaacaggc gtggaggagt
     5221 ccgtcatcgc ttcgcctctt cgagatagaa cggatacacc aggttggcca gcgtgttggt
     5281 tgcgcgcacg cgatacgtga tgtggatcaa cagcgtccgg cgaagcgggc tgtccacagt
     5341 gacccgcaac tccaccacat cgatgcgagg ttcaaaatcg atcaacgcct cgcgtacgcg
     5401 tgtttcgacg cggtggacga tggtagcgtc ggcgggctcg aaaacgaact ggcggagccc
     5461 cgcgccgaaa tcggggcgca tcacgcgttc tcccggattg gttcccaaaa tgacgcggat
     5521 cgcctggcga atatcctctt cgtaagccac cgtcgcgagg ttgccgtgca catccgcttg
     5581 cggcgggaac gccagcccga caccgaggaa cgctttgttc ggatccagtt tctccgcagc
     5641 cacatcagcc tcctatgagc acggtgggta agcccatcac gatgggggcg ccgcatcccg
     5701 tgatgtcgcc catgcgagcc gccggccggc cgccgatcag gaccgtcgcg cttccggcgg
     5761 caatcggatt cggcggatgg ggaccggccg tgggcggcat cgcgcacgca tgcaggtcac
     5821 ccagaacggc ggcaggcaat cctccaatca gcacgttcgg cacgccaggc cccgagatca
     5881 ttccagggtg tccggtcgga tcggttacgc gagcggcggg cggcaaggca tcctcctcag
     5941 ttgatcttca cgagaccgcc ggtgatgctc accgccggcg cggtaatgtt gagaacaccg
     6001 gaggccgtca ggttcaggtt cggcgtgccg gagatggtga tgccgtcggg cgacatatta
     6061 atggcgttgc ttccggagac cacctgcgcg gtgggtggcc cggtcggcgg tccgagatta
     6121 attacattcg cgccggaaag aatctgaatg ccggtgggag cgatcagaat ggtctgcccc
     6181 gtggccatca cctgaatggt gatgctcggc ggcgcttcgg taatcatgat ctggtttccc
     6241 accagcgttt tgatgagccg gatctgcggc gcgggcgagg gcagcggcgg cggagacatc
     6301 gcgttccata agctcccgat gatgtaggga acccgcatgt cgccgtgctc aaacgcaacc
     6361 agcacctcgt cgccgatgtt cgggatcatg tacgtaccgt gcagcggccc cgccatggga
     6421 acggcgattc gcgcccaggg gctcaggtcg acgctgtcgc tgaacggcag ttggacctgt
     6481 accctgccga gcatcagcgg atcgaggatg ttgatcacac gccccgtcac cacgccgtag
     6541 tgctttttgt tcaacctttc gatggaactg tctatccggt ccaataccgt ttcgatcacg
     6601 gaatgatctc cttctttact tcgaagctgg ttctgtaacc gctgccgtcg atggtgtgcg
     6661 tcgcgctcga cacacgatag ttgccgctga aatcgggtcc cagaccttca attccgatca
     6721 gggcaccggc gcgaatgcgt ggatcgccga tgcacgaacc ggagccggtg aggcggttgt
     6781 tcagcttgtt gcgaagctcg ctggcaatga tcagcgcgct gttggtgatg tccgccgggc
     6841 tgccgatggg ctgatcgatc agcgtgagca gcgaaccgag caggctctgg gtcgccgatg
     6901 ccgccgcgcc tggcacaacc gaaatgacga tgctttcgcg gtcgaaatcg tagccgatgg
     6961 ccacgagaaa atcgaggggg atctcgcgca acacgaatcg catcgcaacc cccgccacct
     7021 ggccgatggt gctgacgcgc ggcgaaaagt cgaacaggtt tttaccgtat gtcagggtga
     7081 gacgcggcgt gtactctttg aggaagcgtg agagataaag tacgtttcca tccacccaga
     7141 aatccgcatc gtaagtggcc gcgatttctt cgagaagctc gaaatcactt tgcccgcgct
     7201 gcctgcggct tgcgcctcca aacacgtaat tgacaaccgc cagcgccgtc gatcccgcga
     7261 caatcgtcgg gtcgatggcg ggaatcagca gattctcggc gctcatgatg gaggcgataa
     7321 tggcatccgg caaaaagccg aaaccgcgtg aatacttgcc gcgctggagc cggttcagat
     7381 aatcgtgcgc caccagttgc atggtgggca tgccgccgtt ggggaacgtc acactcacac
     7441 cggtgatgtc accctcgaac atgttttcga gttgtccggc cgcgtatccg acggcgagcg
     7501 tcagcttgtt gtcgaggtca aacgtgccct ccggcgcggc ctgacccagc ttcaccggtc
     7561 ccagagaaat cgcgctcgga aaaggccgga acccgagccc ccggatgtgg ttctggagca
     7621 ggcgcaggtt gggatttgca attccaagct cgacgcggtc cgccgcctgc ctgccatcct
     7681 cataccgcac gctggtaacg gcggaacgca cctgcgcggg catatcctgc ccgttgatct
     7741 gaatccggaa ctccggtgcg taagtggcgt actccggcat tattcgagca cctctccgct
     7801 ttcgggatcg atgaacggaa gcggcggcac catcagggat tgccccgtga tgagcgcgcg
     7861 cggattctcg attccgttga caatagcgat gggccgccac aacagcgggt tgtcatacag
     7921 gcttgccgcg atgccgctga gcgtttcgcc ctgcttcacg atgtgcaact tcacaaagtc
     7981 ggcggtctgc cggttgatct ccttggcttc gcgatcggca tcgatgaatt cgttgaaggt
     8041 gacgttgata cgaactcgcg cgggccttcc atccgggaaa aacaggataa acttctggct
     8101 ggcgcgcgcc agtacgca
//
LOCUS       DASTMK010000103        20417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_37374, whole genome shotgun sequence.
ACCESSION   DASTMK010000103 DASTMK010000000
VERSION     DASTMK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20417
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37374"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1138
                     /locus_tag="VFQ79_04095"
     CDS             164..1138
                     /locus_tag="VFQ79_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138862.1"
                     /translation="MMDLNYLQPLQNQHAYAEWKGKNTLGEDLFVWRLIATGIELEGW
                     QAESMRRLENSGILTERNAEADNLEAPGVGPATLWTWVRPRDGGVSALQVQRVPAAGR
                     SSVPGPRAALQTTSRPYLALTETLWHPSAGPNSALMSAWIYECSSRTAAHQLLLQSLA
                     QFQLPGMVRTEDTAVGDVAFALPSGALMLFARGNLVVLVRNAGRQVVPVIEHARRLDE
                     HLISKPEAVKADAAPQPRKVMFAPVEARVNTPVPVLLAATEQDLLQLHTPAERLVMYK
                     VFARGGELSETHGQLVYTPAAKGQQEVNVFAIDEDRVATRQRLRLTVK"
     gene            1206..2543
                     /locus_tag="VFQ79_04100"
     CDS             1206..2543
                     /locus_tag="VFQ79_04100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0138863.1"
                     /translation="MPTSTPGQWLNPALLEEIAASEMRNAEIPGAAVAVIEGDAPVFL
                     RGFGVAKVSGEESITPETVFQTGSVTKIFTAAALAVLAHQGRVDFHQPAGLYVEGLHP
                     ALARLTLHQLLTHTAGLNDNEAPFAGVHDEEALAAGLKTWNETSILTSPGRIFSYANP
                     GYSLAGLVIQSVTGKKFADAMHELLFEPMGLRHTTFRPSLAASEPLAIGHIASQGATI
                     ALRRFANNVQYWPAGYLYSSAADLGRFVIALMNDGRLDGSQVLPSGILATIAEPRVKP
                     PTGIYQSYGYGIGMLEVNGVQAAGHDGAITGFSARLLMVPERRFAVIVLTNKHLSILG
                     RTAQTAFQMAFPPQPRNGRERRALLPVTSEDIARHAGIYRHGPVRIEILEKDGRLVYK
                     STSLELPMYKLDEARFAVLPPKSKTPTAIVFVSAEGGETIFLHRDMRAFIKVE"
     gene            complement(2478..3143)
                     /locus_tag="VFQ79_04105"
     CDS             complement(2478..3143)
                     /locus_tag="VFQ79_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEU0138864.1"
                     /translation="MWRALLDTPGVPVAGLWESLSASERARAERFQSPEHRSRFVAMH
                     GVLRAILGAYLHLSPGDLRFTYSALGKPEVENTLGLRFSASRSQNIGLFAVTLARAIG
                     VDVEQVRPIEDAERIATAFFHPAEYEELMRTPEPVRLEMFFALWTKKEAYVKALGTGL
                     SLPLESFSVVGPPAEQWSLYELAPDAGYAGAVALEGCRAGLLNLYESSHIAMQKNRLA
                     SLG"
     gene            complement(3209..3433)
                     /locus_tag="VFQ79_04110"
     CDS             complement(3209..3433)
                     /locus_tag="VFQ79_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HEU0138865.1"
                     /translation="MLDDREDLAIYKVVVNHEEQYSIWPLDRENPLGWNDAGKTGRKQ
                     ECLDYIKEVWTDMRPLSVRKQMEQLKRVPG"
     gene            complement(3509..4933)
                     /locus_tag="VFQ79_04115"
     CDS             complement(3509..4933)
                     /locus_tag="VFQ79_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU0138866.1"
                     /translation="MTMLTNDQVVDLAIIAVLLIGVAQFRSVRRARAGNLIAAAALAC
                     SAWLVVYRYGLIDPSVAAAGLALGLLAGAWLAAKVNMVQIPAMIAFQHGAGALAAVLI
                     SFVELTRLPAPDTAVRTLSGVLGMVVGALTFTASMVASGKLSKRLKQRPSFLPRHNTM
                     LLAGVALAVFLSILAARTPGAGYAYWLVLLAAVSGVLGVLFSMRVGGADMPVLISFLN
                     ATAGLAAAFCGVAIGNRLLIAAGATVAASGSILTYVMCKAMNRTLGHVLMPGKAPVQD
                     TRATEADRGTDAQSAVSVTKATKTSDPLPAAVEAASAATSIIIVPGYGMAEGQAQHET
                     VLLANQLVSMGKEVRFAIHPVAGRMPGHMHVLLAEADADPDLLIEIDEANQTFRNTGL
                     VIVVGACDVVNPAAITSTGTPISGMPILRADEAHNVVVCNLDSRPGYSGVENPLYDKA
                     NTILLLGDARVTLRQLREALEAAK"
     gene            complement(4957..5271)
                     /locus_tag="VFQ79_04120"
     CDS             complement(4957..5271)
                     /locus_tag="VFQ79_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU0138867.1"
                     /translation="MADLYIMLAVFFVSLAIGFMIIAKVPPRLHTPLMSMTNAISAVT
                     ILGAFLIFSVETSSWVKLLGLVAMGLAAFNVVGGFVITDRMLRLFRRHERQAGTESVE
                     GG"
     gene            complement(5277..6464)
                     /locus_tag="VFQ79_04125"
     CDS             complement(5277..6464)
                     /locus_tag="VFQ79_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU0138868.1"
                     /translation="MIIGIPREILENEYRVAALPESVAEYRKMGFDVLVETAAGAGVH
                     RSDSEYAAAGAEIASSASDVFSRSDIILKVKQPSVNRGTAKHEVLMMREGGLLITFLH
                     PAAPANHETVRMLRDRNITSLTMDGIPRISRAQQMDALTSMSTVTGYRSVLLAATHLP
                     KFVPMIGTAIGTVKPARFLVIGAGVVGLQAIATAKRLGAIVEALDIQEPARKAAQSLG
                     AKIAGFEVPPEIAADASGAARALPAESLDREREAIAAALERADAVIASALVPGEMAPV
                     LITAAMVSRMKPGSVIIDVSIDQGGNCALSEAGHEVVREDVFLCGLLNIPGSMPVDAS
                     WLYANNVLRYVQNLFKKGIETPDFEDDIVQHTLVTRQGRIVHAGALKAMEVSDPSPTG
                     APR"
     gene            complement(6555..6971)
                     /locus_tag="VFQ79_04130"
     CDS             complement(6555..6971)
                     /locus_tag="VFQ79_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0138869.1"
                     /translation="MPNNSVSNSGTTKVLIVDDETSLLNLLEAYLTRMGFEVTACTRA
                     ADGLEIFENDPQRYSFVLADMNMPDMSGEELVRRVLKASENVRVLVCSGFPVDVSKFG
                     AAGRVGFVQKPFLPRDIEQAIRSLGESAHGADSAAR"
     gene            7016..7429
                     /locus_tag="VFQ79_04135"
     CDS             7016..7429
                     /locus_tag="VFQ79_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU0138870.1"
                     /translation="MQFHTKYLTFQTAKHREYINITPEVEKALAASGIREGMILVSAM
                     HITAGVWVNDAEDGLIADIDEWLEQLAPFRKNYRHHRTGESNGDSHLKSLLVHHQVIL
                     PVTAGALDLGPWQQVYYAEFDGQRPKRVIIKVMGE"
     gene            complement(7431..7994)
                     /locus_tag="VFQ79_04140"
     CDS             complement(7431..7994)
                     /locus_tag="VFQ79_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138871.1"
                     /translation="MSFDPTRFTNEVASILALDGNGERLMPLAGGSCSSAEARKRLQA
                     AGARKLFPEARAPEAALAGLWLYFSCLDECHNIAQQIGSAEGSFWHGIMHRQEPDAGN
                     AGYWFRRVGQHPIFTDLHARALEIVTRFPRSGIRLASEWDAYQFIDICERARRQAASA
                     LEQAALEIQRAEWQLLFDYCARPLPQA"
     gene            8007..9788
                     /locus_tag="VFQ79_04145"
     CDS             8007..9788
                     /locus_tag="VFQ79_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138872.1"
                     /translation="MTDPRREYIRRLKTRRSEANAHQRWNRRIGHLRLATGVAAVLIM
                     WLAASPGLLSPWWLAAPVIVFIGLAVYHERVIAHQRRNLRAAVYYKRALERIEDRWHG
                     KGASGERFLDASHPYAQDLDIFGHASLFQLLCLARTRAGEQTLAEWLLQPATAGEILL
                     RQQAVEELRARIDLREGLAVLGEDFRVGVHPDALASWGAGEPVLRSSTTRIVAALLAL
                     SAVATAVIWAQTGWPLPFLMVLLIEAAFALRLRERVLAVAHAVELPAHDLDLLSQVLV
                     RFEQEEFTSPKLVALKKALETDGLPPSRQIKKLHRLIELLDSRDNLAVRVIGPPLLWT
                     TQIAFAIEAWRKTSGPAVARWLHAVGEIEALSSLAAYAYARPADVFPEFVSDSPTFEG
                     EGLGHPLLMESKVVRNDVSFGKDRRVLIVSGSNMSGKSTLLRTVGTNAVLAMAGAPVR
                     ARRLRLSSLAIGASIRVTDSLQSGSSRFYAEITRIRQLMDLAAGGKPLLFLLDELLHG
                     TNSHDRRIGAEAIVRTLVERGAIGLLTTHDLALADIAGVLNPAAENVHFEDHLENGRI
                     SFDYTLRQGVVRKSNALELMRSVGLQV"
     gene            complement(9785..13069)
                     /locus_tag="VFQ79_04150"
     CDS             complement(9785..13069)
                     /locus_tag="VFQ79_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU0138873.1"
                     /translation="MSTEKLNSSDRRLIWVVLFVSVLSLIYTWANFNAAFPQASIDLK
                     LSKQQITARASEYLRTRNLDPSGFRNLTLFAPQQEASIYLERELGLEEANRLMRDRVA
                     VWRWRARWFRPPEKEERIVQLSPGGNMVGFEHVIAENAPGARFSRDEALAIAEGFLKG
                     QTQAPHKLVEDQRVDRPNRSDYIFTWEENNFRAKDATYRRTIVVQGKNVGKYDEFLHV
                     PEAWRRSYAALRSQNALWAQAASAVYLLLVIVSLIVLFNAVRRREIPWDAVLLIAGAV
                     GVLMVLTNWNSLPLALNEASTSTPLGETVTMMLLLGIASGVGMFLYVALGAAAGAPLY
                     RQAFPAHLPLSVALSPRALRTKEFFLATITGYGFAAAHIAFVVAFYLIGRKFGIWSPQ
                     EVQYSNLLSTYLPWLFPLTISLLAATSEEFWFRLFAIPLLRKWVRFTWLAVVLPAFVW
                     GFLHAGYPQQPPYIRGIEVGVIGVAAGYLMIRFGILATLVWHYTVDAVLIGMFLFQTD
                     SWYFRISGFVVAGAVAIPLILSIVYYRRQNGFQILEAPDTREAAAIEPSPSEAPAPIA
                     PVSTPVAPSWPLRRLYAAAALGLVAVIFLQPRPFGDFIQVRLTRGGAHAAADGEMRRR
                     NLDPGAWTRAVQFTPNLNSASFEYIRRIADSETAEKAVREHTRTAIWRIRYFKPAQKE
                     EWRIYVDQSGAVVRTDHLLDEKAAGAQLPPEQARSIAEDWLTGQRIPVNRWSLVDSQT
                     RKRENRTDHTFVWENPDFRIGEARVRWEVQVIGGEPSFQRSFIKLPEQWLREFERPRI
                     QRFFAPGLIGVLVAVAFVLFLKSLSAGGYTYHTRTYLWVILTTMVLNIAGTVSGWPGM
                     YNGYDTAMPWNTFIVGMVIGLCILVLVLALAVLLGVVALDVFRQSVTTARRLATAPLM
                     RAASVTVLIWGFNVCAKRLIGWVPGPRYSLDLWSLPNASGFFPAIEIVAGAARSAVLW
                     LALVVIAVVIAVRLFRPSLRWAVAGAVAALLAVSGAATILQWTAYVAVAFLALGLLIL
                     IVKTGGPDLVTYGVSLFWLGAIPKAITLAGQHASWLRWNGVMAFVLAAFIGLAALWMA
                     RARNAS"
     gene            13147..14028
                     /locus_tag="VFQ79_04155"
     CDS             13147..14028
                     /locus_tag="VFQ79_04155"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU0138874.1"
                     /translation="MVLLETSIPQMERHARGKVRDIYRIDGQLLIVATDRISAFDYIL
                     PTGIPDKGKVLTRLSIFWFDFLRDVIPTHFITADVSHYPDPLPASRDQLEGRSMLVKQ
                     AEMIEIECVARGYLSGSGWKEYRQSGTVCGIPLPAGLKESDKLPEPIFTPATKAQSGH
                     DENISFQTAEKLVGEDLARQLRDRTLEIYTRAARYAETRGIIIADTKFEFGFVNGELV
                     LADEVLTPDSSRFWPLDEYKPGGAQPSYDKQFVRDYLESIRWDKQPPAPALPDDVAQK
                     TAEKYKSAYRVLTGKEL"
     gene            14025..14582
                     /locus_tag="VFQ79_04160"
     CDS             14025..14582
                     /locus_tag="VFQ79_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU0138875.1"
                     /translation="MNWFDIVLLIIVGSSTVAGFLKGFVRVALGLVATVAAFLLSLGF
                     YRMAGGWFTPVVDSPNTAKLLGFVTIFIGVLLLGWLAGILLSRLLRAVGLSWVDRLGG
                     AVAGAARGIVFAIVILTIVVTFKSDPRFVAESQYAPYVVRASHVAVQFAPRELNDAFH
                     SGYQRLRQAWSEVKKGVPKLPVERM"
     gene            14626..17994
                     /locus_tag="VFQ79_04165"
     CDS             14626..17994
                     /locus_tag="VFQ79_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0138876.1"
                     /translation="MSRLASLVCSGVCLVASVMAQSDRGTITGRVLDPTQSAVPGATV
                     AAANAATGIKYTATTNDTGNYSIQQVPAGTYEVTIEASGFRRYVQRAVELNVAQTATL
                     NVTLDVGQVEQTIEVTESAGVIESSTSDLGTVISRERMIDLPLSVSANMRSPEQFIFL
                     APGVTGNARDTQINGSQSRAKEVLLDGVSSVSPESGGILFTYPSVESIGEFKLLSANF
                     SAEYGRTGGGFEIFTTRSGGNDFHGAVFDYFRNDVFDARGFFANTTPVNRQNEFGAVL
                     GGPIRIPGVYDGRNRSFFHFVYGGFRYRAGATNQLFSIPPMDFRDGDFSRLGRPVYDP
                     ATTRVEDGRTMRDVFPGNIIPRDRFSSVSQQILPLLPQPANSGILNNFLSVGAQRFSR
                     DQIDLKVDHAFSDRNRISGFAYIGTQNNVDPERLPVPFTSALDRDYRSRWIRINHDFI
                     VSPSTLNNFRIGFTREAEYWQKLSAGEDWANRLGIAGLVASEGTFPQVQFTDGLTTWA
                     DDTKSVGQQANNAWQITETLSHIRGNHSLKFGADARWLQTNGADFFLSQGMFRFNALE
                     TGLPGVANSGHAFASFLLGAVDRAEQNVVAIVPGNRYRYLALFAQDDWKATRKLTLNV
                     GLRYDLFFPRTEAHDNLSGFAPDVPNPAAGNRLGAIAFLGDGAGRNGRSSFVDTYYRN
                     FGPRFGFAYSLTEKTILRGGYGLYYAPGNATAGLRSSQSFGFGFNSRPTPASLDNGVT
                     PAFNWDNGFPGGFALPPTIDPSVANGSDVNYIGSDDGRPPYFQNFLFSIQRELPSRIL
                     VEASYVGIKGTRLGTNLIDINELDPRYLALGNLLTRPANSQEARDAGISLPYPGFTGS
                     VAQALRPYPQFNRIQQRSNPNGNSTYHALQTKFEKRFSRGVTFMANYTWSKTISDSNV
                     QAGGGPAGQTYYNRALEKAISTEDVPHIVNIAYTYELPFGPGKPFLNTVGALGKVVGG
                     WQFTGIHQYSSGKPVVLSADNRLPLFNQGLRPDVVSGETRQLAGGGFDPQTDFWINPQ
                     AFAVPGAMQFGTSARSYTDLRAPAFYNESFGLIKRTAITETVQLIFRAEFFNAFNRVV
                     FAAPSSNRSAGNFGRINGQANTPRQGQLALRLEF"
     gene            17995..18666
                     /locus_tag="VFQ79_04170"
     CDS             17995..18666
                     /locus_tag="VFQ79_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0138877.1"
                     /translation="MDLVIFDLDGTLIDSKLDLAHSVNAARGYMGLPPIDNEVVFSYV
                     GNGAPVLMRRAMGPEASEQDVAKALEYFLEYYRRHMLEYTRFYPGVREALDLLRGDGV
                     TMAVLTNKPVRISQDILKGLGLGDHFFRVYGGNSFPQKKPDPIGVDTLAEETGIAKNL
                     TLMVGDSGVDIRTARNAGIKVCGVTFGFQPESFEENPPDFLVDSMEEVANIVLSERRK
                     AKVNS"
     gene            18663..18827
                     /locus_tag="VFQ79_04175"
     CDS             18663..18827
                     /locus_tag="VFQ79_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138878.1"
                     /translation="MTYKPGSPVPEGGIYWCTVCKLPARFNEGQMFPECENMCGRGRW
                     ELAPEEKPEP"
     gene            18832..19782
                     /locus_tag="VFQ79_04180"
     CDS             18832..19782
                     /locus_tag="VFQ79_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138879.1"
                     /translation="MTLLLLSSLCLLQAAPPVPFERLRQLFEYDRSQPAAVTVSLNSE
                     RDGVKVYDFSFDSPVEGRVSGLLVVPPGKGRFPVILYGHWMMTGSPLRNKAEFLDEAI
                     VMARAGAMSVLLDSPLVRKEMPEETDPMEGRGPHAQVQMAKEWRRALDIVLARQDADP
                     GRVAYVGHSFNAGVGAKLAGIEKRITSFVLMANQYSLREFIYDEQNPVMVAERKKRGE
                     AWIQAYFDKFPWDDSRPFAERSAPAAVFLQYGRKDEPLPPSAARLSFNRFAEPKQMEF
                     YDAGHELNAAARVNRAKWLASRLKLKAPDVEALNRIAPLR"
     gene            complement(19807..>20417)
                     /locus_tag="VFQ79_04185"
     CDS             complement(19807..>20417)
                     /locus_tag="VFQ79_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0138880.1"
                     /translation="LVRLPANGREQVEYLIKQVLDLGVFGVVVPHVRNREDALAAVRA
                     MRYAQPEGSAIAVPEGHRGVGYGWAVRYWGLTARDYVAKADLWPLNPNGELLLWCMIE
                     SREGVEHADEIAATPGVSGIFLGPSDLAVSLGVTEDHPKVEQAIQRVVDACKKSGVAC
                     GTLTGSAGVARRLEQGFRFLAVGSDSGISAGVQRSLEIGRKK"
BASE COUNT         4214 a         6225 c         6038 g         3940 t
ORIGIN      
        1 cggccgccgc atacaccgat gctgccggcc aacatccggt ttggcgagcc gggggacggc
       61 cagtatcgcg accggcttgt ggcgcccgcc ggacgctcga attgccagcc gcaaccgctt
      121 gcaacccggg ttcgcaggtg ggttatctag acttaagagg caaatgatgg acctcaatta
      181 cttgcagccg ctacaaaatc agcacgccta cgccgaatgg aagggaaaga acaccctcgg
      241 tgaagacctg ttcgtgtggc gattgattgc cactggaatt gaactggaag gctggcaagc
      301 ggaatccatg cggaggctcg agaactccgg gattttgaca gaacggaacg ctgaagcgga
      361 taacctggaa gcgccggggg tgggtcctgc gacattgtgg acttgggtgc gcccgcgaga
      421 cggcggtgtg tctgcccttc aagtgcagcg cgtcccggca gccggccggt cttctgttcc
      481 cggacccagg gccgccctgc aaacaacgag ccgcccttac ctggctctaa ccgaaacatt
      541 gtggcatcca agcgccggcc ccaattcggc cctgatgagc gcgtggatat acgaatgctc
      601 atcccggacg gctgcccatc aacttctatt gcaatccctg gctcagtttc agttgccggg
      661 aatggtccga acggaagata ccgcagtggg ggatgtcgcc ttcgcgctac ccagtggagc
      721 gctcatgcta tttgcacgag gcaacctggt cgttctcgtc cgcaacgcgg gccggcaggt
      781 ggtcccggtg attgagcatg cgcggcgttt ggacgagcac ctgatttcga agccggaagc
      841 cgtgaaggcg gatgccgcac ctcagccgcg gaaggttatg ttcgccccgg tcgaagctcg
      901 cgtaaacaca ccggttcccg ttcttctggc cgccacggag caggatctgc tgcaactgca
      961 caccccggcg gagcgtctgg ttatgtataa agtgtttgca cgcggtgggg aactgtcgga
     1021 aacccacggg caattggttt atacaccggc ggccaaaggc cagcaggaag tgaatgtttt
     1081 cgcgatagat gaagatcgcg tcgcaaccag gcagcggttg cggctcacgg taaaataacc
     1141 cggcaattcg caaaatgcgg tgttcttgta ttactctgtt tttcgtttcc atcatcgttt
     1201 tgggcatgcc aacgagcacc cccggccaat ggctcaatcc cgccctgctg gaagagatcg
     1261 cggcttccga aatgcggaat gcggagatcc caggagccgc ggtggctgtc atcgaaggag
     1321 acgctccggt ttttctccgc ggctttggcg tcgcgaaggt gtccggggaa gaatcgatca
     1381 ccccggaaac ggtattccag accggctcgg taaccaagat tttcacggcc gcggcgctgg
     1441 ccgtgcttgc tcaccagggc agggttgatt tccatcaacc tgccggcttg tatgtcgagg
     1501 gcctccatcc tgccctcgcc aggctcacgc ttcatcagtt gctgacccac acagccgggt
     1561 tgaacgataa cgaagctcct ttcgcgggcg ttcatgatga ggaggcgttg gctgccggtc
     1621 tgaaaacctg gaacgaaaca tcgatcctca cctcacccgg acggatcttt tcctacgcca
     1681 atcccggcta ttctctcgcg ggactcgtga ttcagagcgt cacaggtaaa aaatttgccg
     1741 atgccatgca cgaattgcta ttcgaaccga tgggattgag gcacaccacc ttccgcccct
     1801 ccctcgccgc atcggagccg ctggcgatag gccatattgc cagccagggc gcgaccatag
     1861 cgctgcggcg gtttgccaac aacgttcagt actggccggc gggatatctg tattcaagcg
     1921 ccgccgattt ggggcgcttt gtcatcgcgc taatgaacga cggcaggctg gatggttccc
     1981 aggttcttcc ctccggcatt ctcgccacca tcgcggaacc ccgggtaaaa cctcccaccg
     2041 gtatttatca gagctacggt tacggaatag gaatgctgga ggtgaatggg gtccaggcgg
     2101 ccgggcacga cggagcgatc accggatttt ccgcgaggct gctgatggtc cccgagcgca
     2161 ggtttgcggt tatcgttttg acgaataaac atctctcgat tctgggacgg acggctcaaa
     2221 cagcatttca gatggccttc ccgccacagc cgcgaaacgg aagggagcga agagcgctgc
     2281 tgccggtgac ctctgaagac attgcacggc acgccggaat ctaccgtcac ggtccggtgc
     2341 ggatcgagat cctggagaag gacggccgcc tcgtctataa gagcacgagc ctggagttgc
     2401 cgatgtacaa gctcgatgaa gcgcgctttg ccgtgctgcc gcccaaaagc aagacgccca
     2461 cggctattgt gtttgtctca gccgagggag gcgagacgat ttttctgcat cgcgatatgc
     2521 gagctttcat aaaggttgag taagccggcc cggcaccctt ccaaagccac ggcaccggcg
     2581 tatccggcat cgggagccag ctcgtagagc gaccattgtt ctgccggcgg gccaaccacg
     2641 ctgaaggatt cgagaggcaa cgaaagcccg gttcccagcg ctttcacgta ggcttctttc
     2701 ttcgtccaga gggcgaagaa catctccagt ctcaccggct ccggcgtccg catcaactcc
     2761 tcgtattccg ctggatgaaa aaaagcggtg gcgatgcgtt ccgcatcttc gatcggccgc
     2821 acctgttcga catccacgcc aatagcgcgg gccagcgtga cggcaaaaag tccgatgttc
     2881 tgcgatcgcg aggcgctgaa gcgcaaccct aaggtattct ccacttcggg ctttcccaaa
     2941 gccgagtacg taaaccgcag atctccgggt gaaagatgga gataggcgcc cagaatcgct
     3001 cgcaggactc cgtgcattgc aacgaaccgc gaccggtgct ccggagactg gaagcgttcc
     3061 gcgcgcgcac gctcacttgc ggaaagcgac tcccagaggc cggcaacggg cacgccgggc
     3121 gtgtccagca aagcccgcca cacatgaaca tcaccaggcg ggaggacgag gccggcagga
     3181 ggggtccgcc atgccggatg gcccgggttc accccggcac tcgcttcaat tgctccatct
     3241 gctttctgac gctgagcggc cgcatgtcgg tccagacttc cttgatgtag tccaggcact
     3301 cctgcttacg cccggtcttc ccggcatcgt tccaccccag cggattttcc cggtccaggg
     3361 gccagattga gtattgttcc tcgtgattga ccacgacttt gtagatggca agatcttctc
     3421 tatcgtccag catgcgcgcc cccttcgtca caccagaata gaagcaaata cggtgcttgg
     3481 caagggcttg agatctacgt tgaagatgct acttcgcggc ttcgagcgct tcccgcaatt
     3541 ggcggagagt cacacgggca tctcccagga gcaggattgt gttcgccttg tcatagagtg
     3601 gattttcgac tcccgagtat ccgggtcggc tgtccaggtt gcagaccaca acgttatgcg
     3661 cctcgtcggc gcgcaaaatc ggcattcccg agatgggggt tcccgtcgac gtgatggccg
     3721 cggggttcac cacgtcgcag gctcccacga caattaccag gccggtattc cggaaggtct
     3781 ggttggcctc atcgatttcg atcaacaaat ctggatcggc gtctgcttcc gccagcagaa
     3841 catgcatgtg gcccggcatc cgcccggcaa ccggatgaat ggcgaaccgg acctctttac
     3901 ccatagatac aagctggttc gccagcagta cagtctcgtg ttgcgcctgc ccctccgcca
     3961 tcccgtatcc cggcacgatg atgatactgg tagcagcgct tgccgcttct accgcggcag
     4021 ggagcgggtc gctggttttg gtggctttcg tgacggacac cgcactctgc gcgtccgtac
     4081 ctctatccgc ttcggttgcc cgcgtgtcct gtaccggcgc cttacctggc atcagaacgt
     4141 gacccagcgt tcggttcatc gctttgcaca tcacataggt gaggatggac cccgaagcgg
     4201 ccacggtcgc cccggcggca atcagcagcc ggttgccaat ggcaacgccg cagaatgccg
     4261 cagccagtcc ggcggttgcg ttcagaaagg aaatcagcac gggcatgtca gcgccgccga
     4321 ctcgcataga gaacagcacg cccaacacac ccgaaactgc ggccagaagc acaagccaat
     4381 acgcataccc ggcgccggga gtgcgggcgg cgagaatcga caggaacacg gcaagcgcaa
     4441 cacccgcgag gagcatcgta ttgtgccgcg gaagaaacga tggcctctgc ttcagccgct
     4501 tcgaaagctt gccgctggcc accatgcttg cggtgaacgt gagggctccc accaccattc
     4561 cgagcacgcc ggagagagtt cgtaccgccg tatccggcgc gggcaggcga gtcagttcga
     4621 caaaggagat caatacggct gccagggctc cggcgccatg ctgaaacgcg atcatcgcgg
     4681 gaatctgcac catattgacc ttcgccgcca gccaggcgcc ggcgagcaaa ccgagcgcca
     4741 ggccggccgc ggcaacactc ggatctatca gcccataccg gtacaccacg agccacgcgg
     4801 aacaggccag tgcggcagcc gcaatgagat tgcctgcgcg cgcccttcga acgcttcgga
     4861 attgcgcaac tccgatcagc aggacagcaa ttatggcaag gtctacaacc tggtcgttcg
     4921 tgagcatcgt catcgtgaat gttcaggggc gtggtgtcag ccgccctcca cgctttcggt
     4981 cccggcctgg cgctcgtgcc tgcgaaacag gcgtagcata cggtctgtaa tgacaaatcc
     5041 tcccacaacg ttgaaggcgg ccaaccccat ggctacaagc cccagcaact tcacccacga
     5101 actggtctca accgaaaaaa tgaggaatgc gcccagaata gtgaccgccg aaatcgcatt
     5161 ggtcatggac atgagaggcg tatgcaatcg cggcggcacc ttggcaatga tcataaagcc
     5221 gatggccagc gacacaaaaa agaccgccag catgatgtag aggtcagcca tgtgcgctac
     5281 cggggcgctc cggtgggcga cgggtcggac acttccatcg ctttcaacgc tccggcatgc
     5341 acgattctgc cttgccgcgt caccagggtg tgctgcacga tgtcgtcttc aaaatcggga
     5401 gtctcgatgc ccttcttgaa caggttctgc acatagcgca ggacattgtt cgcgtacagc
     5461 cagcttgcat cgacaggcat ggagccgggg atattgagca ggccgcaaag gaagacgtct
     5521 tcgcgaacaa cctcgtgacc ggcttcactc aaagcgcagt tcccaccctg atcgatcgat
     5581 acatcgataa tcacagaacc cggcttcatt cgggaaacca tcgcagcagt aatcagtacc
     5641 ggcgccatct cgccgggcac cagggcgctg gcaatgacgg catccgcgcg ttccagcgcc
     5701 gctgcaatgg cttcgcgctc ccggtcgagc gactccgccg gcagcgcgcg agccgcgccg
     5761 cttgcatccg ccgcgatttc gggcggcact tcaaacccgg cgatttttgc gccgaggctc
     5821 tgggccgctt tccgcgccgg ttcctgaata tccagcgctt caacgattgc gcctaatcgc
     5881 ttcgccgtcg cgatggcttg aagccccaca acgcccgcac cgatcaccag aaagcgcgcg
     5941 ggcttcactg ttccaatcgc agtgccgatc atgggcacaa acttcggaag atgcgtcgcg
     6001 gcgagaagaa ccgaccggta gcccgtcacc gtactcatcg aggtcaatgc gtccatctgc
     6061 tgcgctctcg aaatgcgcgg gattccatcc attgtgagcg aggtgatatt ccggtcgcgc
     6121 agcatgcgga ccgtctcgtg attggccggc gccgccggat gcaaaaacgt aatcagcagg
     6181 ccgccttcac gcatcattaa cacttcgtgc tttgcggtcc cccgattgac ggacggctgc
     6241 ttcactttca ggatgatgtc cgagcgcgag aaaacatccg atgcgctgga ggcgatctcc
     6301 gcaccggcgg cggcatactc ggaatcagac cgatgcacac cggcgcccgc cgccgtttcg
     6361 acgagcacat caaatcccat cttcctgtac tccgccaccg attcaggtag agccgctacc
     6421 cggtattcgt tctccagaat ttcgcgagga atgccgatga tcatagtccg ctccgttcca
     6481 ttattgcctt tcactcgcaa tcatagcggg aacgaccgcc tgctggcgcg cgcggctcag
     6541 taacggtaac ggagttagcg ggcggcggaa tcggcgccgt gagcggattc accgagcgag
     6601 cggatggcct gttctatgtc tcgtggcagg aatggctttt gcacaaaacc gacgcggccc
     6661 gcggctccga acttcgaaac gtcaacggga aatccgctgc agaccagcac ccgtacattt
     6721 tcgctggctt tgaggacgcg ccggacaagt tcttcgcctg acatgtcggg catgttcatg
     6781 tcggccagaa cgaatgagta tcgctgtggg tcgttttcga agatctcgag accatcagcg
     6841 gcccgagtgc atgcagtcac ctcgaagccc attctcgtca ggtatgcttc gagcaggttc
     6901 agaagcgatg tctcatcgtc gacaatgagg acttttgtag ttccgctgtt ggacactgaa
     6961 ttgttgggca atggatcatt gtcgcacaat caagttaaaa gccagtaata attctatgca
     7021 attccacaca aaatatctca cttttcaaac cgcgaaacat cgtgagtaca tcaacatcac
     7081 gccggaagtc gaaaaggcgc tggccgcgag tggcattcgt gaagggatga tactggtttc
     7141 cgccatgcac atcaccgccg gagtctgggt gaacgatgcg gaagacggac tcattgccga
     7201 catcgatgaa tggctggaac aactcgcgcc atttcgtaaa aactaccggc accatcgaac
     7261 cggcgaaagc aacggagatt cgcatttgaa gagtctcctg gtgcaccacc aggtgatttt
     7321 gccggtaaca gcaggcgcgc tcgacctggg cccgtggcag caggtttatt acgcggagtt
     7381 cgacgggcaa cgcccgaagc gcgtcatcat caaagtgatg ggcgagtagc tcacgcttgc
     7441 ggcaacggcc gggcgcaata gtcgaacagc aactgccatt cggcgcgttg tatttcaagc
     7501 gccgcctgct ccagcgccga tgcagcctgg cgccgcgccc gctcgcagat atcgatgaat
     7561 tgataagcgt cccactcgga cgccaggcgg atgcccgacc gcggaaaccg cgtgacgatc
     7621 tcaagcgcgc gagcatggag atccgtaaaa atcgggtgct gccccactct gcgaaaccag
     7681 tagccggcgt ttccggcatc gggttcctgc cggtgcatga tgccatgcca gaaactgcct
     7741 tcggcggagc cgatctgctg agcgatattg tgacattcgt ccagacacga gaagtacagc
     7801 catagtccgg ccagggccgc ttcgggcgcg cgcgcttcgg gaaagagttt gcgggcgccc
     7861 gctgcctgaa ggcgcttccg cgcttccgcg gacgagcagg agccaccggc aagcggcatc
     7921 agccgctctc cgtttccatc gagcgccaga atggacgcca cctcgttcgt gaaccgggtc
     7981 ggatcgaagc tcataataat aagatagtga ccgatccccg ccgagagtac atccgccgtt
     8041 tgaaaacaag gcgctccgaa gcaaacgcgc atcagcgatg gaaccgcaga atcggtcatc
     8101 tgcggctcgc caccggtgtc gccgcggtgc tgatcatgtg gctggcggcc agcccggggc
     8161 tgctatcgcc gtggtggctg gcggctcctg tgattgtttt catcgggctg gcggtgtatc
     8221 acgaacgcgt aatcgcgcat cagcgccgca acctccgggc cgctgtttat tacaaaaggg
     8281 cgctcgagcg tatagaagac cgctggcacg gcaagggagc atccggcgag cgctttctgg
     8341 atgcctcgca tccctacgca caggatctcg acattttcgg acacgcctcg ctttttcagt
     8401 tactctgcct ggcgcgaaca cgcgcgggag aacagacgct ggcggagtgg ctgctacagc
     8461 cagccacagc cggcgaaatc cttttgcgcc agcaggcggt ggaggaactg cgcgcacgca
     8521 tcgatttgcg ggaagggctg gccgtgttgg gagaggactt ccgggtcggt gttcaccccg
     8581 acgccctcgc ctcgtgggga gcgggagagc cggtattgcg ctcgtccacg acgcgcattg
     8641 tggcagcgct tcttgcatta tcagccgtcg caacggccgt tatctgggca cagaccggat
     8701 ggccgctgcc gtttctgatg gttctgctca tcgaagcagc cttcgcgctg aggctccggg
     8761 agcgcgtcct cgcggtggcg catgccgtcg agcttcccgc ccacgatctg gacttgctgt
     8821 cgcaggtgct ggtgcggttc gagcaggagg agttcacctc cccaaaactc gtggcgctca
     8881 agaaagcgct tgaaacggat ggccttccgc catcacgcca gatcaagaaa ctccaccggc
     8941 tgatagaact gctcgactcg cgcgataatc tcgcggtgcg ggttatcggg ccgccgctgc
     9001 tttggacaac acagatcgct tttgcgatcg aggcctggcg aaaaacatcg ggccccgctg
     9061 tagcccggtg gctccacgcg gtgggcgaga ttgaggcgct atcgtcgctt gcggcctacg
     9121 cgtacgcccg cccggcggat gtttttccgg agttcgtttc ggattctccc accttcgaag
     9181 gggaaggact cgggcatccc ctgttgatgg agagcaaggt ggtccgcaac gacgtaagct
     9241 tcggaaagga ccggcgggtg ttgattgtaa gcggttcgaa tatgtcgggg aagagtacgc
     9301 ttctgcgaac cgtgggaacc aatgccgtac tcgccatggc cggcgctccg gtacgcgcgc
     9361 gcaggctgcg gctgtcgtct ctcgcgattg gcgcttccat acgcgtcaca gactcgctgc
     9421 aaagcggcag ttcccgcttt tatgcggaga tcacgcgcat tcgccagttg atggatctgg
     9481 cagcgggtgg aaagccgctg ctgttcctgc tggatgaact cttgcacgga accaattcac
     9541 acgatcgccg catcggcgcc gaagcgattg ttcgaacact tgtcgaacgc ggcgcaatcg
     9601 gactcctcac cacacatgac ctcgcattgg cggatatcgc cggcgtgctc aatccagcag
     9661 cagagaacgt ccatttcgaa gaccatcttg aaaacggccg gatttccttc gactacacgc
     9721 tccgccaggg cgtcgtgcga aagagcaacg cgctggagct gatgcgcagt gtgggcttgc
     9781 aggtttagga agcgtttctc gcgcgggcca tccaaagcgc cgccaacccg atgaacgcag
     9841 cgagaacgaa cgccatgacg ccgttccacc gcagccagga agcgtgttgg ccagcaagag
     9901 tgatggcttt cggaatggcg cccagccaga acagcgaaac gccgtatgtc accaggtccg
     9961 gaccgccggt cttgacgatc agaatgagta ggccaagcgc gaggaacgcg acggcaacat
    10021 aagcggtcca ttgcaggatt gtcgcggcgc cggaaaccgc aaggagcgca gccaccgctc
    10081 cggcgacggc ccatcgcaga gacggccgga acaaccggac ggcaatgaca acggcgatga
    10141 cgacgagagc cagccagagc acggcgcttc gggcagcgcc ggcaacgatc tcgatggccg
    10201 gaaagaagcc gctcgcgttg ggcagactcc agagatcgag cgagtagcga ggtccgggaa
    10261 cccatccgat gagccgcttc gcacacacgt tgaagcccca gatcagcacc gtgacggaag
    10321 cggcccgcat gagcggcgcc gtagcgagac gccgggccgt tgtcacgctc tgccggaaga
    10381 catccagagc caccactcca agcagaacgg cgagggccag tacaagaacc agaatacaga
    10441 ggccaattac catgcctacg atgaacgtgt tccacggcat agcggtgtcg tagccgttgt
    10501 acataccggg ccagccgctc actgtgcctg cgatgttcaa aaccatcgtc gttaagatca
    10561 cccacaggta tgtgcgagtg tggtacgtgt acccgcccgc gctcaagctc tttaggaaca
    10621 ggacgaacgc aaccgccacc aacaccccta taagaccggg tgcgaaaaac cgctggattc
    10681 gcggccgctc gaattcgcgg agccactgct cgggaagctt gatgaacgat ctctgaaacg
    10741 acggttcgcc gccgatgacc tgcacctccc agcgcacgcg ggcttcgcca atccggaagt
    10801 ccggattctc ccaaacgaac gtgtgatccg tccggttctc gcgcttgcgg gtctgcgaat
    10861 cgacgagact ccagcggttc accggaatgc gctgccctgt tagccaatcc tcggcaatgc
    10921 tccgtgcctg ttcgggcgga agctgcgcgc cggcagcttt ttcatccaga agatgatccg
    10981 ttcgcacgac agctccggac tgatccacat agatgcgcca ctcttctttc tgggccggtt
    11041 tgaagtagcg gattcgccag atggccgtgc gtgtgtgctc gcgcaccgcc ttttcagccg
    11101 tctctgaatc tgcgatgcgg cggatatatt cgaaagacgc agaattcagg ttcggcgtga
    11161 attgcacggc tcgcgtccag gcgcccgggt caagattccg ccgcctcatt tcgccgtccg
    11221 cggcagcatg cgcccccccg cgcgtgagcc ggacttgaat gaaatcgcca aatggccgcg
    11281 gttgaaggaa aatgacggca accagcccga gagccgcggc ggcgtacaac cttcgcagcg
    11341 gccaggatgg cgcaacaggc gttgagacgg gcgctatcgg cgcaggggcc tccgatgggg
    11401 acggctcgat ggcagcggcc tccctcgtat ccggtgcttc taagatctga aatccgttct
    11461 gccgcctgta gtagacaatc gacagaatca gtggaatagc gacagcgccc gcaaccacaa
    11521 agcctgaaat gcggaagtac caactatcgg tctggaacag aaacatgccg atgagcaccg
    11581 cgtccaccgt gtagtgccac accagcgtcg ccagaatgcc gaaccggatc atcagatacc
    11641 cggcggctac tccgattacg cccacttcaa tcccacgtat ataaggcggc tgttgaggat
    11701 agccggcgtg caggaaaccc cagacgaacg cggggagcac aaccgccagc caggtaaaac
    11761 gcacccattt ccgcagcaac ggaatcgcga acagacggaa ccagaactcc tccgaggtcg
    11821 cggccagcag ggagatcgtc aacgggaaca accacggcaa gtaggtagag agcaggttcg
    11881 agtattgcac ctcctgcggg ctccagatac cgaacttgcg cccgatgagg tagaaggcaa
    11941 cgacaaacgc gatgtgtgcc gcggcgaagc cgtagccggt aatggtggca aggaagaatt
    12001 ccttcgttcg cagtgctcgc ggcgatagtg cgacggacaa gggcagatga gccggaaaag
    12061 cctggcggta caaaggcgcg ccggccgccg cccccagcgc gacgtaaaga aacattccga
    12121 cgcccgaagc gatgcccaac agcagcatca tggtgacggt ttcgcccaag ggggtgctcg
    12181 tcgaggcctc attcaacgcc agtggaagcg agttccaatt ggtcagcacc atcaagacgc
    12241 ctactgcgcc ggcaatcagc agcacggcat cccaggggat ctcgcgccgc cggaccgcgt
    12301 tgaaaagtac gatcaacgaa acgatgacca gcaacaaata aacagccgag gcggcctggg
    12361 cccagagggc gttttgggaa cgcaacgccg cgtagctcct gcgccatgct tccggcacat
    12421 gcagaaactc gtcgtatttc ccgacgtttt tgccttgcac aacgatagtc cggcgatagg
    12481 ttgcgtcttt ggcccggaag ttgttttcct cccaggtgaa gatgtaatcg cttcggtttg
    12541 gccggtcgac gcgctgatcc tcaaccagct tgtggggcgc ctgagtctgc cctttcagaa
    12601 agccttccgc gatggcgaga gcctcgtccc ttgaaaaccg tgcgcccggt gcgttttcgg
    12661 caatcacatg ctcgaagccg accatattgc cgccaggtga gagttgcaca atgcgctctt
    12721 ccttttcggg aggacggaac caccgtgcgc gccatcgcca cacagccaca cggtcccgca
    12781 tcaggcggtt cgcctcttca aggcccagtt cacgctccag atagatagag gcctcctgtt
    12841 gcggggcgaa gagcgtcagg tttcgaaagc ccgatggatc gagattccgc gtccggagat
    12901 actccgaggc tcgtgccgta atctgctgtt tcgaaagctt gagatcgatc gaggcttgcg
    12961 gaaacgccgc attgaagttg gcccacgtat agataaggga cagcaccgat acaaaaagca
    13021 cgacccagat caaccgccgg tccgacgaat tcagtttttc ggtggacatg atcaccgcct
    13081 ctcagccgcc gctttgcgcc agccaaactc tccgattctg ttattgtgta ttcaatcgga
    13141 gcgttaatgg tcttacttga aacaagtatt ccgcaaatgg agcgccacgc ccggggcaag
    13201 gtgcgcgata tctacaggat tgacggccag ctcctgattg tcgccaccga ccggatctcg
    13261 gcgttcgatt atatcctgcc taccggtatt cccgacaaag ggaaggtgct gacgcggttg
    13321 tcgatcttct ggttcgactt cctgcgcgac gtgattccca ctcacttcat cacggcggat
    13381 gtttcccatt accccgatcc gctgccggct tcccgggatc agttagaggg gcgttccatg
    13441 ctggtgaagc aggcggagat gattgaaatc gagtgtgtcg cccgcggcta tctctccggc
    13501 tcaggatgga aagaataccg gcagtcaggg accgtgtgcg gcattccgct gccggctgga
    13561 ctaaaagaaa gcgacaagct gccggagccg atcttcaccc ccgcaacaaa ggcgcagagc
    13621 gggcacgacg aaaacatctc cttccaaacg gccgaaaagc tggtgggcga ggaccttgcg
    13681 cggcagcttc gtgaccgcac gctggaaatc tacacccgcg cggcgcgata cgccgaaacg
    13741 cgcggtatca ttattgccga tacgaagttc gagttcggtt tcgtgaacgg agagctcgtg
    13801 ttggccgacg aggtgctgac gccggattcg tcccgattct ggcccctcga tgagtacaaa
    13861 cccggcggcg cccaaccctc ctacgacaag cagttcgttc gcgattacct ggagtcgatt
    13921 cgctgggata agcaacctcc cgcacctgcg cttccggatg acgtagcgca gaaaacagcg
    13981 gaaaagtaca agagcgccta ccgagtgttg actggaaaag agctgtgaac tggttcgaca
    14041 tcgtactgtt gatcatcgtc ggcagttcga cggtggccgg cttcctgaaa ggtttcgtcc
    14101 gggttgcatt gggcctggtc gcgaccgttg ccgccttcct gctcagcctc gggttctatc
    14161 gcatggcggg cggatggttc acgcccgtcg tcgattctcc caacacggcg aagctgctgg
    14221 ggttcgtcac gattttcatc ggtgtgcttc tgcttggctg gcttgccggc atactgctct
    14281 cccgtctgct gcgggcggtg gggctgagct gggtggatcg cttaggtggc gcggtggccg
    14341 gcgcagctcg cggcatcgtg ttcgccatcg ttattctcac catcgtcgtt accttcaaat
    14401 ccgatccgcg gttcgtcgcc gaatcgcagt acgcccccta tgttgtccgc gcctcgcatg
    14461 tcgcggtgca gtttgcaccg cgggaattga atgacgcctt tcacagcggt tatcaacgct
    14521 tacgccaagc ctggtccgaa gtgaaaaaag gtgttcccaa actgccggtc gaacgcatgt
    14581 gaagacaact taaaccgcac taaaggggtt tgatacgatt gatttatgag tcgtctcgct
    14641 tctttggtat gctccggcgt gtgtcttgtg gcgtccgtca tggcgcagtc cgaccgcgga
    14701 acaatcaccg gacgcgtgct cgatccgacg caatcggcag tgcccggcgc aacggtcgcg
    14761 gcagcgaatg ccgccactgg aataaagtac acggccacta ccaacgatac cggcaactac
    14821 tccatacaac aggttcccgc aggcacctac gaggtgacta ttgaggcctc cggcttccgg
    14881 cggtacgtgc agagagcggt agagctcaac gtggcgcaaa cggcgacatt gaacgtgacg
    14941 ctggatgtgg gccaggtgga acagacgatc gaagttacgg aatccgccgg agtaatcgaa
    15001 tcctccactt ccgatttggg tacggtcatc agccgcgagc ggatgatcga tttgccgctt
    15061 tccgtatcgg cgaacatgcg cagtccggag cagttcattt tccttgcacc gggcgtcacc
    15121 ggcaacgcac gggatacgca aatcaacgga tcgcagtcgc gcgcgaagga agtgctgctt
    15181 gatggtgtga gctccgtcag tccggaaagc ggcggcattc tgttcacgta tccttccgtg
    15241 gaatccatcg gcgaattcaa actgctcagc gcgaatttca gtgccgaata cgggcgcacc
    15301 ggcggcgggt ttgagatctt cactacaaga tccggcggca acgattttca cggcgcggta
    15361 ttcgattatt ttcgaaatga cgttttcgac gcgcgcgggt tcttcgccaa caccactccg
    15421 gtgaaccggc agaacgagtt cggcgcggta ctcggcggcc ccatccgcat tccaggcgtt
    15481 tacgacggcc gcaacaggtc cttctttcac ttcgtgtatg gaggattccg gtaccgtgct
    15541 ggtgcgacga accagttgtt cagtattccg ccgatggatt tccgcgacgg cgatttctcg
    15601 cggctcggac gccctgtcta tgatccggcc accacgcgcg tcgaagacgg caggacgatg
    15661 cgcgatgtct tccccggcaa catcatcccg cgcgaccgtt tcagcagcgt ctctcaacag
    15721 atcctgcccc tgctgcccca accggcgaat tccggcattc tgaataattt cctgtccgtg
    15781 ggcgcacaga ggttcagccg cgaccagatc gacctgaagg tcgatcacgc cttcagcgac
    15841 cggaaccgca tcagtggctt tgcgtacatc ggtacgcaaa acaatgtgga cccggagcgc
    15901 ctgcccgtgc ccttcaccag cgcactcgat cgcgactacc gctcgcgatg gattcgcatc
    15961 aatcacgatt tcattgtttc gccgagcacg ctaaacaact tccgcatcgg tttcacgcgc
    16021 gaagcggagt actggcaaaa gctttccgca ggtgaggatt gggcgaacag gctgggaatc
    16081 gccgggctgg tcgccagcga aggaacgttc ccccaggtgc agttcacaga tggactcacc
    16141 acgtgggccg acgatacgaa atcggtaggc cagcaggcca ataacgcgtg gcagatcacg
    16201 gaaaccctca gccacatccg cgggaaccat tcgctgaagt tcggcgcgga tgcgcgctgg
    16261 ctgcagacca atggtgcgga tttcttcctg tcgcagggaa tgttccgctt caacgcgctc
    16321 gagaccggcc tgcccggcgt cgcgaattcg ggacacgcct ttgccagctt cctccttggc
    16381 gctgtggacc gcgcggaaca gaacgtggtt gccatcgttc caggcaaccg gtatcgctac
    16441 ctggcgctgt tcgcgcagga tgactggaag gccactcgca aactgacgct gaacgtggga
    16501 ttgcgctacg acctgttctt cccaagaacg gaggcccacg ataacctgtc gggctttgcg
    16561 ccggatgtgc ccaacccggc ggcgggcaat cgtctgggcg ccatcgcgtt cctcggcgac
    16621 ggcgcgggac gcaatggccg tagcagcttc gtggatacct actatcgcaa cttcggcccg
    16681 cggtttggct ttgcctattc actgactgag aaaacgattc tgcgcggtgg ctatggactt
    16741 tactacgcac ccggcaatgc gaccgccggc ctgcgcagtt cccagagttt cggattcggt
    16801 ttcaactccc ggccgacgcc cgcttctctc gacaacggcg tgacaccggc gtttaactgg
    16861 gacaacggat ttccaggagg cttcgcgctg ccgccgacga tcgatccgtc ggtcgccaat
    16921 ggcagcgacg tgaattacat cgggagcgac gacggacggc cgccgtactt ccagaatttt
    16981 cttttcagca tccagcgtga gctgccttcg cgcatcctgg tggaggcctc ctacgtagga
    17041 atcaaaggaa cgcggctcgg tacgaacctg atcgacatta atgaactgga cccgcgctat
    17101 ctcgccctgg gcaacctgtt gacgcgcccg gcgaattcgc aggaagcacg ggatgcgggc
    17161 atctcccttc cttatcccgg ctttaccggc tcggtggcac aggcgctgcg tccctatcca
    17221 cagttcaacc gtattcagca gcgctccaac ccgaacggta actccaccta tcacgcgttg
    17281 cagacgaagt ttgaaaaacg cttctcgcgc ggggtcacct ttatggccaa ctatacgtgg
    17341 tcgaagacga tttccgacag caacgtacag gcgggaggcg gtccggccgg gcaaacctat
    17401 tacaaccgcg cgctggagaa agccatcagc acggaggacg tgccgcatat cgtcaacatc
    17461 gcctatacgt atgagctgcc cttcgggccc ggaaagccgt ttctgaatac ggtcggcgcc
    17521 ctgggtaagg ttgtgggcgg ctggcaattc acgggcatcc accagtactc ctccggcaag
    17581 cctgttgttc tctcggccga caaccgtttg cctctattca accagggtct gcggcccgac
    17641 gttgtttccg gggaaacgcg ccaactcgcc ggcggcggtt tcgatccgca aaccgatttc
    17701 tggatcaatc cccaggcgtt cgctgtccct ggcgcaatgc aattcgggac gtcggcgcgt
    17761 tcctatacgg acctccgcgc acccgccttt tacaacgaga gtttcggtct catcaagcga
    17821 accgccatca cggaaaccgt gcagcttatc ttccgggcgg agttcttcaa cgctttcaac
    17881 cgcgtcgtgt tcgcagctcc atccagcaac cggagcgcgg gaaacttcgg gcgcatcaac
    17941 ggacaggcaa ataccccgcg gcagggacaa ctggcgcttc gactcgagtt ctgaatggac
    18001 ctggtgatct ttgatcttga cggcacgctg atcgattcca aactcgatct ggcgcactcg
    18061 gtcaacgcag cccgcggtta catgggactg ccgccgatcg acaacgaagt tgtattctcc
    18121 tatgtcggca acggcgctcc ggtgctgatg cgccgggcga tggggcctga ggcgagtgaa
    18181 caggatgtcg caaaagccct cgaatatttc ctcgagtatt accggcggca catgctcgaa
    18241 tacacgcgct tttatccggg cgtgcgggaa gcgctggatc tgctgcgggg cgatggcgtt
    18301 accatggcgg tgttgacgaa caagcccgtc cggatcagcc aggacattct gaagggactc
    18361 ggcttgggcg atcacttttt tcgtgtgtat ggcggcaaca gctttccgca gaagaaacct
    18421 gatccgattg gcgtggatac gctcgccgaa gaaaccggga tcgcgaaaaa cctcactctg
    18481 atggtgggtg acagcggagt ggatatccgg accgcgcgca atgcgggcat taaagtctgc
    18541 ggcgtgacgt tcggattcca gccggagagc ttcgaggaga atccgcctga tttccttgta
    18601 gacagcatgg aagaggtggc gaatatcgtg ttatcggagc gcagaaaagc gaaggtgaat
    18661 tcatgaccta caagccgggt agtccggttc ccgaaggcgg tatttattgg tgcacggtat
    18721 gcaagctgcc ggcccgtttc aacgaagggc agatgtttcc cgagtgcgag aatatgtgcg
    18781 gacgcggccg ctgggaactc gcgccggaag aaaaaccgga accctgacgg tgtgacgctc
    18841 ctcctcctct ccagtctttg cctgcttcaa gcggctcctc ctgtgccgtt cgaacgtctg
    18901 cgccagctct tcgaatacga tcgcagccaa cctgcggctg tgaccgtttc gcttaactcc
    18961 gaacgggatg gcgtgaaagt gtacgacttt tcgttcgaca gcccggttga aggccgtgtc
    19021 tccggcctgc tcgtcgtgcc ccctggaaag ggccgctttc cggtgatcct gtatgggcac
    19081 tggatgatga cgggctcgcc gttacgaaac aaggccgagt ttcttgacga agcgattgtc
    19141 atggcgcgcg ccggcgcaat gagtgtactc ctggattccc cgctcgtacg gaaagagatg
    19201 cctgaagaaa cggacccgat ggaaggccgt ggtccacacg cacaggtgca aatggcaaag
    19261 gaatggcgcc gcgcattgga tatcgtgctg gcccgccagg acgccgatcc tggccgcgtc
    19321 gcttatgttg gacacagctt caatgcgggt gtcggagcaa agctcgcagg catcgaaaag
    19381 cgtattacct ccttcgtgct catggccaat cagtactcgc ttcgcgagtt catctacgat
    19441 gagcaaaacc cggtcatggt agccgaacgc aagaagcgcg gcgaagcgtg gatccaagct
    19501 tacttcgaca agttcccatg ggacgattcg cggccgttcg ccgagcggtc cgcgccggca
    19561 gccgtctttc ttcaatacgg gcgcaaggac gaaccgttac cgccaagcgc cgcacgcctg
    19621 agcttcaacc ggttcgctga gccgaagcag atggagtttt acgacgccgg gcacgagttg
    19681 aacgcagccg caagagttaa ccgtgcgaag tggctggcct cccgcctgaa gctgaaggcg
    19741 ccggatgtag aagcactcaa ccggatcgcc ccgctgcgct gatggcaggc cgcctcgcgc
    19801 agagtgctat tttttccggc cgatctccag tgatctttga acgccggcgc tgatgccgga
    19861 atccgagccg acagcgagga agcgaaagcc ctgttccagc cgccgggcga cgcccgcact
    19921 tccggtgagc gtcccgcatg caactcccga cttcttgcag gcatcgacga cgcgttgtat
    19981 ggcttgttcc actttcgggt ggtcctcggt gacgccgaga gaaacagcga gatccgacgg
    20041 tccgaggaag atgccgctaa ctcccggtgt tgcggcgatt tcgtccgcgt gctcgacgcc
    20101 ttcgcgcgac tcgatcatgc accatagcag caactcaccg ttcgggttga gaggccacag
    20161 gtctgccttg gccacgtaat ccctggccgt caggccccag tagcggaccg cccaaccgta
    20221 gccgacccct cgatgtcctt ccggaacggc gatggcggac ccttccggct gcgcatatcg
    20281 catcgcacgc acggccgcca gagcatcttc gcgattgcga acatgcggca cgacgacgcc
    20341 gaatacaccc agatcgagta cctgcttgat gagatactcc acctgctcgc ggccattggc
    20401 cggcaagcgt accaaag
//
LOCUS       DASTMK010000104         9682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_420919, whole genome shotgun sequence.
ACCESSION   DASTMK010000104 DASTMK010000000
VERSION     DASTMK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420919"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFQ79_04190"
     CDS             <1..312
                     /locus_tag="VFQ79_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU0138881.1"
                     /translation="ADEVSANQAIQMVTDIAAVAEVGKTYLGKVVRLVDFGAFVEIFP
                     GTDGLLHISEISENRIKNVRDELKEGDQILVKVLALEGNKIKLSRKAVLREQRDKLKK
                     T"
     gene            complement(317..1402)
                     /locus_tag="VFQ79_04195"
     CDS             complement(317..1402)
                     /locus_tag="VFQ79_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU0138882.1"
                     /translation="MAHWFIRFVILMALPGLVAAGAERIETIVGTGSPGYFGDGGPGV
                     EANINNPYGLTIGPDGALYFCEIDNHCIRRLDLKSRTITTVAGSLRPGYSGDGGPARE
                     ASLHQPYEVRFDSAGNMYFVEMRNHLVRRVDARTRKISTVAGTGTPGFSGDGGPAKKA
                     QLRNPHSIVFDRQGRLLICDIGNHRIRRVNLKTGIIETFAGTGEKKPTPEGALAAAAP
                     LNGPRAMDFSPDGSLFVALREGNAVYRIDARTGRIHHVAGNGELGYSGDGGPAVEAKL
                     AGPKGIAYGNDRALYIADTESHTVRRIDLKTGKISTAVGTGRKGDGPNGNPRECKLAR
                     PHGVFVDRSGTIYIGDSENHRIRIVRR"
     gene            complement(1426..2259)
                     /locus_tag="VFQ79_04200"
     CDS             complement(1426..2259)
                     /locus_tag="VFQ79_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU0138883.1"
                     /translation="MKTLLIRPGAIGDFIVSLPALECLRTQYTEVWCSDRNVPLSGAF
                     DRARSIASTGLDLAGLEGVEPPPSLFEALAGFDSIVSWYGANRQEFRSAMHEAGLPFR
                     FFPALPPEGAACHAVDYYLEQAASIRACNAAVRVPRIHCPRTPGNFAVIHPFSGSARK
                     NWPLDRFRVLSEWIGQRMPVAWCAGSEEPLDNAARFDNLYELACWLASARLFLGNDSG
                     ISHLAAAAGTPSVVIFGPTDPRVWAPTGECVQIVKTKTPGEAIEAVDVADVMRAVAPL
                     C"
     gene            2321..3547
                     /locus_tag="VFQ79_04205"
     CDS             2321..3547
                     /locus_tag="VFQ79_04205"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0138884.1"
                     /translation="MRLGILALALSACLYGQSRYDLLLKGGHVIDPANQRDGVMDVAV
                     KGNTIARVASAIPAGEAVKVLDVSGLYVTPGLIDLHAHVYGYSGSIFPDDTALIAGTT
                     TVVDAGGAGWRTFDDFYEKIIRPSRTRVLAFLNIVGRGMLGGDVESNVDDMDPERTAA
                     KIRERRDYIVGIKTAHFALPGWVAIDRAVKAGELSGTPVMVDDKILTGAERDTRTKLL
                     EKLRPGDMHTHMYNDRQLELVDRFTGRVQPYVLEARKKGVLFDLGHGAGSFLWPVAVK
                     AMRQGFAPDTISTDLHSSSIMIAESDMPNCISKLMNLGMTLQEGIAKSTVRPAEVIRR
                     YPELGTLGEGRVADISVFELRTGVFAFKDAWHKKMLGNKKLEAVATIRDGKLVYDRKG
                     LGFPEWTKAGDYGVIP"
     gene            3544..4767
                     /locus_tag="VFQ79_04210"
     CDS             3544..4767
                     /locus_tag="VFQ79_04210"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0138885.1"
                     /translation="MKARVVLLTALVSMPAAAQPIYDLLLKNGHVIDPANGRNGRFDI
                     AIIGDRIAAIGDSLPASHARQVVELGEFYVTPGLIDIHAHFDAQGAWLNLNPDHNTLR
                     NGVTTAVDAGSSGWKNFEAFKDTVIDHAQVRLLAFLNIVGAGMYGSKVEDDVAEMDPD
                     AAARMVKKYPELVVGIKTAHFQPATWDAVDRAVKAGELSGTPVMVDFHPKPGRGYEEL
                     ILKHMRPGDIHTHFYGRLTPQLDDSKKVQPYMWQARKRGVLFDVGHGSGSFWFRIAAP
                     ALKQGFLPDTISTDIHKDSIMLSRATMPNVMSKLLNLGATLEQVVERSTITPAKAIRR
                     PELGLLKEGGVADIAVFSLDRGRFEFLDSGRGKLTGDKNLRCVLTVRKGKVVWDSEGL
                     SLTGWSEAGPYSNYK"
     gene            4886..7507
                     /locus_tag="VFQ79_04215"
     CDS             4886..7507
                     /locus_tag="VFQ79_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0138886.1"
                     /translation="MLTQSVSRYQIQDRLGEGGMGVVHKAVDRKLHRPVALKFLPLDA
                     ISNPEYVQRFMQEAQVISALNHPSIATIYEFEQMENSLFLVLEYVPGGDLRSYIRETY
                     GAGKEVPFSEVLRLAIQIARGLDHAHRRGVVHRDIKSSNILLTTDKDAKITDFGLAKW
                     TESSLHTTTGEFRGTLAYVAPEQAEGKTVDERADIFSFGVVLFELASGRRPFEGETAA
                     ALLYSIATKPAPRLSEYRPDTPPEFDQIVARALEKKPADRYQSVRQLLDALEAARVHL
                     GHDRSTVARSMVSAPLLAFRRSRVWLVALLLAVAMASTTFLLSPSIREALGNWSVNWN
                     SPANKHLAILPFHSVGANRADTAFADGLAEILASKLTQMEQFHGEVIVVSTAELRREG
                     VTSPREARAAFGATLALAGSLQPLGDDLVLALSLVDTKSGLQLGARTLTSSKSRPAAL
                     EERAFEAIVDLLRFQLRPESRKALHARATAVPGAYDHYLQGRGYLLRFDRMEDVDRAI
                     AAFNQSVAIDPDYALAYAGRAEAYWWKYVRERDSQLLERVREDCRRAMSLDSNLAAVH
                     MTMGLVNTGTGRFAAAVDDFQHALGLEPANPDALRELGKAYDAMGETAKAEAEYRRAI
                     DLRPNYWAGYKDLGIFHFKHGRQEQAEACFRRVIELTPDNYVGYSNLGAVLLKQGRYQ
                     EAAETLERSIAVKPTAKALSNLATLYYFRKQYAEAAAVYKQATLMTPSDYVMWGNLAE
                     AYRLVPERAADAADAYRKAIEAAEQHARINPRDAHTRALIAAYWSFLGDHAEAVRNIQ
                     SALELGPEDGFVRFRAAVVFENSGRREEALKQLALAISYGYSRDEIAEATPLVALRAD
                     PRYARLARAPDVVPSPR"
     gene            7527..7862
                     /locus_tag="VFQ79_04220"
     CDS             7527..7862
                     /locus_tag="VFQ79_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138887.1"
                     /translation="MFENKPIDVEITVLGEGNFNYGYALLRIAPDQPVRWVSKSGPFV
                     ISFGRESPFEKVDYRGVPDRETGLAVTEPAVVRKENRIGSFKYYVAVCVGDKVYIDAQ
                     CPVIVVEGR"
     gene            complement(7859..8413)
                     /locus_tag="VFQ79_04225"
     CDS             complement(7859..8413)
                     /locus_tag="VFQ79_04225"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0138888.1"
                     /translation="MPHRNPWKKLDSRLVYENAWMRVREYQVVRPDGNHGIYGVVEVS
                     PSVGIVALNEHEEIVLVGQWRYPNERYSWEIPRGGSKSHDKDPLEAAARELREETGLV
                     ARHWRALGAVDVCNGLTNDVQHLFLATGLSHEVPAHDPEENLSIRWVPYRQACEMALG
                     GEITEVCSIAAILKIRLLCDGEGC"
     gene            complement(8471..9445)
                     /locus_tag="VFQ79_04230"
     CDS             complement(8471..9445)
                     /locus_tag="VFQ79_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0138889.1"
                     /translation="MDRRAFLGAISSAAMHGQSSNNQAGSEERRKQLYALMGDLPARN
                     RPIAAKTLRTEDRGAYLLETLELDLNGIEPVPAYFVKPKKQTGRIPAILYHHAHGGDY
                     ALGKDELLRGRSALQKPPYAELVTSLGYCALCTDTWAFGQRAKRPEMDIFKEMLWKGQ
                     VMWGMMVYDSLRAMDYLAGRPEVDNTRMGTLGLSMGSTMAWWQAALDPRAKVTVDICC
                     LTDYQALMETNNLKGHGIYYYVPSLLKHFTSAQINALIAPRAHLSLAGNLDALTPPKG
                     LGRIDRELRSVYEKLGHPERWKLFRQDVGHQETPEMRVEIVAFLKRHL"
BASE COUNT         1961 a         2888 c         2939 g         1894 t
ORIGIN      
        1 gccgatgaag tctcggcaaa tcaggccatc cagatggtca ccgacattgc agccgttgcc
       61 gaagtgggca aaacgtacct gggcaaagtt gtccgcctgg tcgatttcgg ggctttcgtc
      121 gaaatcttcc ccggtacgga cggactgctg cacatcagcg agatttccga aaaccgcatc
      181 aagaacgttc gcgacgaact gaaggaaggc gatcagattc tggttaaagt gctcgcgctc
      241 gaaggaaaca aaatcaagct cagccgtaag gctgtcttgc gcgaacagcg ggacaaactc
      301 aaaaagacct gacggtttat cgccgcacga ttctgatgcg atggttctcg ctatcgccga
      361 tgtagatcgt gccggagcgg tcgacgaaga caccatgtgg ccgcgcgagc ttgcactcgc
      421 gcggattccc attgggtccg tctcctttcc tgcccgttcc caccgccgtt gagatcttcc
      481 cggtcttcag gtcgatccgg cggaccgtgt ggctctcggt gtcggcgata tacagtgccc
      541 gatcgttacc gtacgcaatc ccctttgggc cggcgagttt cgcctctacc gcggggccgc
      601 catcgcccga ataccctaat tccccattgc ccgcgacatg gtgaatgcgg ccggtccggg
      661 cgtcgattcg atacaccgcg tttccttcgc gcagtgcgac aaagagactg ccgtccggac
      721 tgaaatccat ggctcgcgga ccgttgagag gcgcagccgc tgccagggct ccctcgggag
      781 tgggcttttt ctcgcccgta ccggcaaagg tctcgatgat gcccgttttc agattcacac
      841 ggcggatacg atgattcccg atatcgcaga tcagaagtct gccttgccgg tcgaaaacga
      901 tgctgtgtgg atttcggagt tgggcttttt ttgccggccc gccatcgcca ctgaatcccg
      961 gtgtgccggt tccggcgacg gtactaattt tccgtgtccg cgcatccaca cggcgaacca
     1021 aatggttgcg catctcgacg aagtacatgt tgcccgccga atcgaatcgc acttcgtagg
     1081 gctggtggag cgatgcctct ctcgcaggcc cgccatcgcc tgaatacccg ggccggagac
     1141 tcccggcaac ggtcgtaatc gtccgggact tcagatcgag ccttcgaatg cagtgattgt
     1201 cgatttcgca gaagtataga gcaccatcgg gaccgatcgt gaggccatat gggttattga
     1261 tgttcgcttc cacgcccggg ccaccgtcgc cgaagtatcc tggagaaccg gttcccacaa
     1321 tggtttcgat gcgctccgcg ccggcggcca ccagacccgg caaggccatc aatatgacaa
     1381 aacggataaa ccaatgtgcc attccggccc tcttcacaag gaagtctagc agagcggcgc
     1441 gacggccctc atcacatcgg ccacgtcaac ggcttctatc gcctcccccg gcgtctttgt
     1501 cttgacgatt tgaacgcact ctcccgtcgg cgcccacacg cgggggtcgg tcggcccgaa
     1561 aattacaacg gacggtgttc cggccgccgc ggccaggtgt gagattccgg aatcgttgcc
     1621 taggaataat ctcgctgaag ccaaccagca ggcaagctcg tagagattgt cgaagcgcgc
     1681 ggcgttgtcg agaggttcct cgcttccggc gcaccacgcg acaggcattc gttgcccgat
     1741 ccattcacta agtacccgga accggtcgag gggccagttc ttccgtgcgc ttccggagaa
     1801 cggatgaatc acggcgaagt tgccgggcgt tcgcggacag tgaatccggg ggactcgcac
     1861 ggccgcgttg cacgcgcgaa tcgacgccgc ttgttcgaga tagtagtcca cggcatgaca
     1921 cgcagcccct tccggtggaa gcgccgggaa aaatcggaac ggcagtcctg cttcgtgcat
     1981 cgccgaacgg aactcctgcc ggttcgcgcc ataccacgag acgatggaat cgaacccggc
     2041 gagtgcctcg aacagcgatg ggggcggctc aacaccttcg agcccggcga ggtctaatcc
     2101 ggtcgaagca atggaccgcg ccctgtcgaa cgcgcctgaa agcggaacat tccggtctga
     2161 gcaccatacc tctgtatact gtgtgcgcag gcattccagc gccgggagcg agacaataaa
     2221 gtccccgata gcgccgggcc gtatcagaag agttttcaca ggctggcaac acgcagtata
     2281 cttgccggaa gctcccgccc gcggggcaaa aaggaaaagg atgagactgg gaattcttgc
     2341 gctcgctctg agcgcctgcc tgtacgggca atccaggtac gacttgctgt tgaagggcgg
     2401 ccatgtcatc gacccggcca accagcgcga cggcgtcatg gatgtggcgg tgaaggggaa
     2461 caccattgcc cgcgtggcgt ccgcgattcc cgccggtgaa gccgtcaaag tgctggatgt
     2521 ttccgggttg tacgtgacgc cggggctgat tgatctgcat gcccacgttt acgggtactc
     2581 cgggtccatt ttcccggacg ataccgctct gatcgccggc accaccactg tggtcgacgc
     2641 cggcggagcc ggatggcgca ccttcgacga tttttacgaa aagatcatcc ggccatccag
     2701 aacacgtgtg ctcgcttttc tgaatatcgt cgggcgcggc atgctgggcg gcgatgtgga
     2761 gagcaacgtc gacgacatgg atcccgaacg cacggccgct aagatccggg agcggcgcga
     2821 ttacatcgtc ggaatcaaga cggcgcattt cgccctgccg ggatgggtcg cgattgaccg
     2881 tgccgtgaaa gctggcgagc tttccggcac gcccgtcatg gtggacgaca agatcctgac
     2941 cggcgccgaa agagataccc gcacgaagct gctcgagaag ctgcgtcctg gcgacatgca
     3001 cacgcacatg tacaacgatc gtcaattgga gctggtggac cgcttcactg gccgtgttca
     3061 accctacgtc ctcgaggcca gaaaaaaagg cgtgcttttc gatctggggc acggcgccgg
     3121 cagcttcctc tggcccgttg cggtgaaggc catgcggcag ggcttcgcgc ccgataccat
     3181 cagcaccgat ctgcattcgt cgagcatcat gatcgccgaa tccgacatgc cgaactgcat
     3241 ctcgaagctg atgaacctcg gcatgacgct tcaggaaggc atcgcgaaat cgaccgtcag
     3301 accggcggaa gtaatccgcc gctaccccga attgggcacg ctgggcgaag gacgcgtcgc
     3361 tgatatctcc gttttcgaac tccggaccgg cgtgtttgcc ttcaaagatg cctggcacaa
     3421 gaaaatgctg ggcaataaga agctcgaggc agtggctacc attcgcgacg gaaagctagt
     3481 ctatgaccgc aagggcctgg gctttcccga atggaccaaa gcgggagatt acggggtgat
     3541 tccatgaagg ctcgtgtggt tttgctgacc gcgctggtct caatgcccgc cgcggctcaa
     3601 cccatctacg atctgttgct caagaacggg catgtgatcg atccggcgaa cggccgcaac
     3661 gggcgcttcg acattgccat tatcggagac cgcatcgccg ccatcgggga ttcgcttccc
     3721 gcgtcgcacg cgcggcaagt ggtcgaactc ggcgaattct atgtcactcc ggggctcatc
     3781 gacatccacg ctcatttcga tgcgcaggga gcgtggctga acctcaaccc cgaccacaac
     3841 actcttcgaa acggtgttac caccgcggtg gacgccggca gttcgggctg gaagaacttc
     3901 gaagcgttca aggacaccgt catcgaccat gcccaggtgc ggctgcttgc gttccttaat
     3961 atcgtcggcg cggggatgta cggctcgaag gtggaagacg acgtggcgga gatggatccg
     4021 gacgccgccg cgcgcatggt gaagaagtac ccggaactgg tcgtcggcat caagaccgcg
     4081 catttccagc ccgcgacgtg ggacgcagtg gaccgggcgg tgaaggcggg ggaactcagc
     4141 ggaacgcccg tcatggtgga ctttcacccg aagcccggcc gcggctatga agagttgatc
     4201 ctgaagcaca tgaggcccgg cgatatccac acgcattttt acgggcgcct gactccgcag
     4261 ctcgacgatt cgaagaaggt gcagccttat atgtggcagg cgcgaaaacg cggtgtgctg
     4321 ttcgacgtcg ggcacggttc gggcagcttc tggttccgga tcgccgcgcc tgctttgaag
     4381 cagggcttcc tccccgacac gatttccacc gacattcaca aggacagcat tatgctgtca
     4441 cgtgccacca tgccgaacgt catgtcgaag ctcctcaatc ttggcgcgac tctggagcaa
     4501 gtggtggaga gaagcaccat cacgccggcg aaggccatcc gcaggccgga gctcggattg
     4561 ttgaaggaag gaggagtcgc cgacatcgcg gtcttctcgc tcgatcgggg gcgtttcgaa
     4621 tttctcgatt ccgggcgcgg caagttgacc ggcgataaga acctgcgctg cgtcctcacg
     4681 gttcgcaagg gaaaagtggt gtgggattcc gagggactga gtttaaccgg ctggagcgag
     4741 gctggtccgt acagtaacta caagtaaccc tttcagcgat gagcatccgc gcctccacgg
     4801 cagtatactg acatcaacac cgggaattgt tggctgtgca gcacggaagc agtgcaaggt
     4861 gtcccggcac ggaccgcaag gcggtatgct gactcagagc gtctcccgat accagattca
     4921 ggaccggctt ggtgaaggcg gcatgggagt cgtccacaaa gcggtcgacc ggaaattgca
     4981 ccgtcccgtc gcgttgaaat tcctcccgct cgatgccatc tccaaccccg aatatgtgca
     5041 gcggttcatg caggaagcgc aggtcatttc agcgctcaat cacccctcca tcgccaccat
     5101 ctatgagttc gagcagatgg agaacagcct ttttcttgtt ctcgagtatg ttcccggagg
     5161 ggacctccgt tcgtatatcc gtgaaaccta cggggctgga aaagaagtgc cattctccga
     5221 ggtgctgcgg ctggcgattc aaatcgcgcg cgggttggat cacgcgcacc ggcggggcgt
     5281 cgttcatcgc gacatcaagt cgagcaatat cctgctgacg acggacaagg acgcgaagat
     5341 cacggatttc ggcctcgcga aatggacgga atcgtcgctg cacaccacta ccggtgaatt
     5401 ccggggcacc ttggcctacg tcgctcccga acaggccgag ggcaaaacag ttgacgagcg
     5461 cgcggacatc ttttctttcg gtgtcgtgct gttcgagctg gccagcggcc gccggccctt
     5521 cgaaggggag accgccgcgg cgctgctcta cagcattgcc acaaagccgg ctccgcggtt
     5581 gagtgagtac aggccggata ccccgcccga attcgatcag attgtcgccc gtgcgctcga
     5641 gaagaagccg gcggaccgct atcagagcgt gcggcaacta ctcgacgccc tggaggcggc
     5701 acgcgttcac ctcggtcacg accggtcaac cgtcgcacgg tctatggtgt cggcgccgct
     5761 tctcgcattc cgccgctcgc gagtctggct ggtggcgctc ctcctggcgg tcgccatggc
     5821 ttccacgacg tttcttctgt cgccttccat acgggaagcg ctgggaaact ggagcgtcaa
     5881 ctggaatagt ccggcaaaca aacatctcgc catccttccg ttccacagcg ttggagccaa
     5941 tcgggcggat accgcgttcg cggacggcct ggcggagatt ctggcaagca agctgacgca
     6001 gatggagcaa ttccacggcg aggtcatcgt agtctccacc gccgagttgc ggcgcgaagg
     6061 cgtcacgagt cctcgcgagg cgcgagcggc gtttggagct accctcgcgt tggccggaag
     6121 cttacagccg cttggcgacg acctggttct ggcgctgagc ctcgtggata cgaaatccgg
     6181 cctgcaactg ggggcccgca cgctcaccag ttccaagtcc cgtcccgccg cgctcgagga
     6241 gcgtgcgttt gaggcaatcg tggatctgct ccggtttcaa ttgcgcccgg agagccgtaa
     6301 agcgctgcac gcgcgcgcca cggctgtgcc cggcgcctac gaccactatc tgcaaggccg
     6361 tggatacctg ctgcggttcg accgaatgga ggatgtggac cgggcgatcg ccgcgttcaa
     6421 ccagtctgtt gccatcgatc ccgattatgc actcgcctac gcggggcgcg cggaagcata
     6481 ctggtggaag tacgtgcgcg aacgcgattc gcagcttctc gaacgcgtcc gcgaggactg
     6541 ccggcgggcg atgtcgctcg acagcaatct ggccgcggta cacatgacca tgggactcgt
     6601 taacacgggc accggaaggt tcgccgccgc tgtcgatgac ttccagcacg ccctgggact
     6661 ggagccggca aatccggatg cgctccggga actgggaaaa gcctacgatg cgatgggcga
     6721 aaccgcaaag gcggaagccg agtacagaag agcgattgac ctgcggccca actattgggc
     6781 cggctataag gatctcggca tcttccattt caagcacggc aggcaggaac aggccgaagc
     6841 ctgttttcgc cgtgtgattg aattgacacc ggataattat gtcggataca gcaatctggg
     6901 agcggtgttg ctgaaacagg ggcgatacca ggaggctgcg gagacactcg agcgctccat
     6961 cgccgtgaag cctaccgcca aggcgctttc caacctggct acgctttact atttcaggaa
     7021 gcaatatgcg gaggccgccg cggtctataa gcaggccacg ctgatgacgc cgtccgacta
     7081 tgtcatgtgg ggcaacctgg ccgaagccta caggctggtt ccagagcggg ccgccgatgc
     7141 ggcggacgca taccggaagg cgattgaagc agcggagcaa catgcgcgta tcaatccgcg
     7201 agacgcgcac acgcgggcct tgattgcggc atactggtcc ttcttgggcg atcacgctga
     7261 agcggtaagg aacattcagt ctgccctgga actcggaccc gaagacgggt tcgttcgctt
     7321 ccgtgcggca gttgtgtttg agaactccgg acggcgcgag gaggctttga aacaactggc
     7381 gctcgccatc agctacggat actcacgcga cgagatcgcc gaggccacgc cgctggtggc
     7441 ccttcgcgcc gaccccagat atgcccgcct tgcccgggcg ccggatgtcg taccatcccc
     7501 gcggtaaaaa ctcaggagaa tacataatgt tcgagaacaa acccattgac gtggagatca
     7561 cggtcctcgg tgaaggcaac ttcaattacg gatatgcact attgcgtatt gcaccggatc
     7621 agccggtgcg ctgggtgtcg aaaagcggac ccttcgtaat ttccttcggc agggaaagcc
     7681 catttgagaa agtcgattat agaggcgtgc cggatcgtga aacgggactg gctgtgacgg
     7741 agccggccgt ggtcaggaag gagaaccgca tcggcagctt caaatactac gtggcggttt
     7801 gtgtgggcga caaggtctat atcgacgccc agtgtccggt gattgtggtg gaaggacgct
     7861 agcagccctc accatcacac agcagcctga ttttgagaat ggcggcgatg gagcagactt
     7921 cggtaatctc cccgccaagc gccatttcgc atgcctgccg gtatgggacc cagcggatag
     7981 aaaggttttc ctccgggtcg tgtgccggca cttcatgcga caaccccgtc gccagaaata
     8041 agtgctgcac atcgttggta agaccgttgc agacatccac ggctcccagc gcgcgccaat
     8101 ggcgggccac taggcctgtc tcctcgcgaa gctcgcgagc ggcggcttcg agcgggtctt
     8161 tatcgtggga cttcgagcct ccgcgcggaa tctcccagga gtatcgctca ttcggatagc
     8221 gccattgccc aaccagcacg atctcttcgt gttcgttcaa cgccacaatg ccgacggacg
     8281 gagaaacttc aacaacgcca tagatgccgt ggttcccatc cggacgcacc acctggtatt
     8341 cgcggacgcg catccaggca ttctcgtaga cgagtctcga gtccaatttc ttccagggat
     8401 tgcggtgggg catctatgat ttcagtgtaa cggaccggta ccggcacgct tgctggcgcg
     8461 cggctccgtt tcagagatgc cgttttagaa acgccacaat ttcgacacgc atttccggtg
     8521 tttcctggtg gccgacgtcc tgccggaaca gtttccatcg ttccggatgg ccgagcttct
     8581 catataccga tcgcagttca cggtcaatac gaccgagccc cttgggtggt gtgagcgcat
     8641 ccaggttgcc ggcgaggctc aaatgcgcgc gcggcgcaat cagcgcattg atttgggccg
     8701 aggtgaagtg cttcaacagc gatggaacgt agtagtagat tccatgcccc ttcaggttat
     8761 tcgtctccat cagcgcctgg taatccgtga ggcagcagat atcgacggtc acttttgcgc
     8821 gcggatccag ggccgcctgc caccaggcca tcgtgctgcc catggagagg ccaagagttc
     8881 ccatgcgcgt gttgtccacc tcgggccggc cggcgagata atccattgcg cgaagtgaat
     8941 cgtacaccat cattccccac atcacctggc ccttccacaa catctctttg aagatgtcca
     9001 tctcgggccg cttggcacgc tggccgaaag cccaggtgtc cgtgcacagc gcgcagtagc
     9061 cgagcgaagt cacgagctcg gcatagggcg gtttctggag cgcgcttctg ccacgcagca
     9121 attcgtcctt gccgagtgcg taatctccgc cgtgcgcgtg atggtagaga atcgccggta
     9181 tacgcccggt ttgtttcttc ggctttacga agtatgccgg aaccggctcg atgccattca
     9241 aatccagttc gagtgtctcc agcagatatg cgccgcggtc ctccgtgcgg agcgtctttg
     9301 cggcaatagg ccggtttcgt gcgggcagat cgcccatcag agcgtaaagc tgtttccgcc
     9361 gttcctctga accggcctgg ttgttgctgg attgtccgtg catggctgcc gaagaaatcg
     9421 cgcccaaaaa ggctctgcga tccatttggt tctcgattgt atcgcatgaa acccggtagg
     9481 gcgtgtgtga cctcggtcac tggcttaaac gccggcaggg tgcatccttg gaaagagagg
     9541 tggcgaaacc tgggaacatc gtccgccatc ccgctgaata acgaactcag aggagaaata
     9601 gcgtggcaga cattgagaat gcgcgagtca aagagttgga cttgcgcccg ataccgcacg
     9661 gaagaaggca tggcctggta tt
//
LOCUS       DASTMK010000105         9233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_421141, whole genome shotgun sequence.
ACCESSION   DASTMK010000105 DASTMK010000000
VERSION     DASTMK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421141"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFQ79_04235"
     CDS             <1..616
                     /locus_tag="VFQ79_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138890.1"
                     /translation="TKHGHPPGEETLCGELAMTSRVRQALDAAGMRDTIIYIEETPPD
                     AAAPYFDAAFCYNLPLAKPTPLPVKLNLWRFAFPDVRLWDMLSIGIEPRSLPAEDFRL
                     SLWHGNGLWLKGHADTWYGEDLLRFVRRAHGILKEHAASFAGTADPLVASPHPAVFVN
                     RFRGEEEMVYTLFNASYRTVRFTFQERERTLPPRDVDVVVETHR"
     gene            705..2228
                     /locus_tag="VFQ79_04240"
     CDS             705..2228
                     /locus_tag="VFQ79_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138891.1"
                     /translation="MKGGEDGALSRRTFAGAAGFLIVPRHVLGGAGYVPPSDRVTLAA
                     IGMGRQGMAVTMELLARPDVQVVAVCDCNQGSKNYAEYGANALLTSARRLLGPGYEKW
                     GEDLASPGSLQLTHSFQTSLGMGGRDPARRMVEAFYSSRKASAYKGCASYRDYRELLE
                     KEKDVDAVYVATPDHWHAPISLAAMRKRKHVLCQKPMTHSIGEARRMAQMAREMKVAT
                     SLPVNNPSSDATKFISEWIADGAIGRVREVHNWSSRPFWAQGVDRPAEQQPVPEGLDW
                     DLWLGPAPSRPYNKAYLPFVWRGWYDFGCGSFGDMGCYSFAGLFKILDLTPPTAVEAC
                     SSESFEETYPKASIVHLDFPAKGSRPALRLSWYDGGLKPPRPAGLSQEDQAMFDRGRE
                     GIMYVGDKGILLGGFNGDNPRVYPESPKYKLPPRERGARERDRATDQWLAAAKGGPAP
                     LTNFEIQAPATEAFLLGCMAQRLAGSRLEWDTAKMRVTNLEKANPYVDPPYRSGYDV"
     gene            complement(2216..3271)
                     /locus_tag="VFQ79_04245"
     CDS             complement(2216..3271)
                     /locus_tag="VFQ79_04245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0138892.1"
                     /translation="MTGKPAFANPLVTVTIVTFDSRKYIERCLDSIYEQDYRPIEVIV
                     VDNASTDGTAELLMEYEDLVRVLYNYENTGFAAAQNQAIAAGHGEWVLTLNPDVRLFP
                     GFIERLVEAASSGSGVGTVCGKLLRAAPDLSVPRERRIDSTGIHFTPSQRHFDRGWNE
                     PDDGRFNTPEYVFGATAAAALYRREMIEDVSLDGDFFDPDFFTYREDADIAWRAQLMG
                     WRCLYVPSAMGCHVRRISAANRRSAPPVLRMHSVKNRFLMRIKNMTGDLYHRHWKSIT
                     MRDVLVTGGCLLAEPACLPAFWRLACLFPRALAKRREIMKRCTVTDEYMAAWFSEAPA
                     SKPLHVPATDHAAVHTS"
     gene            3381..3929
                     /locus_tag="VFQ79_04250"
     CDS             3381..3929
                     /locus_tag="VFQ79_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0138893.1"
                     /translation="MLSDSIHREALGIARSEGSATPLQDRVARLFEAWREDVYRYLLT
                     LGLYPPQAQETTQEVFLRLYVVLRQNQNIENPRAWVFRVAHNLGLKIRSEESWQQPFD
                     PAMEERIAAGATPEKSLLQKERALRFRQAVESLSERQKRCLFLRLEGLRYAEIGAVLG
                     ISASTVGEFMRRAIAKLRASNE"
     gene            3922..5271
                     /locus_tag="VFQ79_04255"
     CDS             3922..5271
                     /locus_tag="VFQ79_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0138894.1"
                     /translation="MNSFEAGHPADGDLLCYLDGELQGRDVKRMEDHLAACWQCRTEL
                     QELQHTIAECVQYRKTVNSSLPEPPAPWFDIYRRFDQIDTVGTPVMVRLWNVFRPIVV
                     RPQPWAAAAVLALAVWAAVDHFRREAEPTPAKPAPVGTVRAPLPVTEAPSVPVPQAQR
                     VETPRREIEQPPTPGDELRVLAVLRRLDADLGEPVEVTRGSERVLVAGIGVAPHLQRQ
                     IEQELEGMPRVMVHFSEPAAVMPPYVAGTPGAAARRTDLAALESRIETHLGGRGAYEQ
                     FASDVLDTSDAAMSRVHALHRLAEQFPPEIESKMTPDERALLTGLRLEHAAALRDVSA
                     ALNARAMPVLSLFGGQPLPHQAPAPALSWQAAAEQLFREARNMESLLGAMLGGAATHS
                     SAADLPVRILSSLAAVHAHAQFQIGTDSPAGMTSMPSGHTGQGRSKRAPRRVSKSIE"
     gene            5277..8327
                     /locus_tag="VFQ79_04260"
     CDS             5277..8327
                     /locus_tag="VFQ79_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0138895.1"
                     /translation="MRTSRRHCDRLIPLCILFATLLSAQQQTHISGWIRDPSGAGVPA
                     ASISVVDEQTGFRRTTYSQMSGAYAVASLRPGTYKITVRKQGFRTLIRFGAELVAAQL
                     TQMDFDLSLGSMQEVITVHGSPPLLNNESVSVGTLIRRETIDALPLNGRGLLSLLELA
                     PGAIATPATRGEAGQFSASGQRPNANYFTLDGVSANTGVSGGGLPAQCTGGSLPGMTA
                     LGSLHGLVSLEALDEFRIETSSAAPESGTLPGARVSLSTRSGTNQFHGSLFHYFRDEH
                     LDANGWFANQAGEPRAQLNMYDLGASLGGPLKRNRSFFFVSYEGMRLRGPFAWRAPVP
                     SAEARNNAPEWARPVLGLLPLPNGRWLDPNTAEWAGSNYRRSRFDVAGLRLDHAFTSR
                     LTAFARFNYAPSSNQFTGTQVNDLQMRSRSVTVGMNLRLNTAAILDLKANVSDAKAES
                     FWRAAESAGDSSCFIGPVTEFFVRTEGLCNYLLRFSISGIGQALWGREADQRQTQWQV
                     LPSAILVLGSHQFRIGFDRRRFAPIRRDNNGSISVVAEDLDDLLKSRNLWVSVAAPRT
                     LRSAMEEMAAYAQDTWRVHPRLTATIGLRWEYSRPPQLTQGTFYQSQGDWQHSGAYIF
                     PSQSEIWKRRYTNFAPRFGVAFQPLGGKALVLRAGGGLFYDSSLSIATDLVNGGPFSV
                     AQYGNPRHAPFSTIMSYGFVPDLRLPAVYQWNFTVEQALPGRNVVSAGYVGASGHHLL
                     RREFGGAGAGETLWLALATNQGQSRYHALQAQYRRPMAHGLEALASYSWSHSIDNSSS
                     DALLHRTGLNLSAGGDRGSSDFDARHTFTAGLTYEVGSKSGDTLWARAWSGWGIDGIL
                     RARSAFPITVLNSEYSMGLSFANAFRPDLVGGQPVWIRDPSAPGRRRLNRDAFQLTDE
                     TVQGSLGRNAIRGFGMQQIDTAVRRQFQLGERWSLQLRIEAFNLLNHPNFSDPVPYLG
                     NPLFGESASMLNLMLGTGSPGSGLTPVFQTGGARSMQIALRLRF"
     gene            8407..>9233
                     /locus_tag="VFQ79_04265"
     CDS             8407..>9233
                     /locus_tag="VFQ79_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138896.1"
                     /translation="MDPKGWSAAHVLPAPDDAAQWPACRDALARWRVDEKRRIGYRDD
                     LYRRPDFSWVPSSFACCFLMLCDEKFYDYRSGRYEVDAWIDVGIREFGGYDSVVLWHA
                     YPRIGLDQRNQFDFYRDMPGGLEGVRRVSQAFQARGIKVYINYNPWDTGTRREGVSDI
                     DAVVAMVKAVDADGIFLDTMNQGSSEFRVKLDEARRGVVLESEGALPSLENIQDHHMS
                     WAQGFEDSAVPGVLRDKWFERRHMQHQIKRWNRDHTGELHAAWMNGSGIMIWENVFG"
BASE COUNT         1698 a         2752 c         2969 g         1814 t
ORIGIN      
        1 cactaagcat ggacatccgc ccggggagga aacgctgtgc ggcgaactgg ccatgaccag
       61 ccgcgtgcgt caggcgctgg atgccgcggg catgcgcgac accatcatct atatcgaaga
      121 gaccccgccg gatgccgccg caccctattt tgacgcggcc ttctgctaca acctgccgct
      181 agccaaaccg acgcccctcc cggtgaagtt gaacctgtgg cggttcgcct ttccggacgt
      241 gcggctttgg gacatgctct ctatcggcat cgagccacgc tcgctgccgg cggaggactt
      301 ccgcctgtcg ctctggcacg gcaacggcct ctggctgaag ggtcacgcgg atacgtggta
      361 cggagaagac ctgctgcggt tcgtccgccg ggcgcatggc atcctcaagg agcacgcggc
      421 gtctttcgcc gggactgcgg atccgctggt agcttcgccc cacccggcgg tcttcgtgaa
      481 ccgttttcgg ggagaggagg agatggtcta cactctcttt aacgcgtcgt atcgaaccgt
      541 gcgctttacg tttcaggaac gggaaagaac gcttcctccg cgtgatgtag acgtggtggt
      601 ggaaacgcac cggtgaacgc ccgcttcctc cgggtgcaca gaagcctgcc cgctgctata
      661 tactcgcagt ggcttgccat tgcaagcctc aatggaggtg ttcagtgaaa ggtggagagg
      721 acggcgcgct gtcgaggcgc acctttgccg gcgcggccgg gtttttgatt gttccacgcc
      781 atgtgttggg cggagccgga tacgttccgc caagcgaccg cgtcacactc gcggcgatcg
      841 gcatgggccg gcaggggatg gccgtcacga tggaactgct ggcacggccg gatgtacagg
      901 tcgtggcggt ctgcgattgc aaccagggca gcaaaaatta cgctgagtat ggagcgaacg
      961 cgttgctgac ttccgcgcgc cggctgcttg gccccggata cgagaaatgg ggcgaagatc
     1021 ttgcatcgcc cggatcgttg cagcttacgc acagcttcca gaccagttta ggaatgggcg
     1081 gccgcgatcc ggcgcgccgc atggtggagg cgttctacag ctcgcgaaaa gcgtcggcgt
     1141 ataaggggtg cgcgtcttac cgggactatc gtgagctgct cgaaaaagaa aaagacgtgg
     1201 atgcggtata tgtcgctacg cccgaccact ggcatgcgcc gatttcactc gccgccatgc
     1261 gcaaacggaa acatgtgctg tgccagaaac cgatgacgca ctcgatcgga gaagcgcgac
     1321 ggatggcgca aatggcgcgc gagatgaaag tcgctacttc gcttccggtg aacaatcctt
     1381 cgagcgatgc gaccaagttc atctccgaat ggattgccga tggcgccatc gggcgcgtgc
     1441 gcgaagtgca taactggtcg agccgcccgt tctgggcgca gggagtggat cgtccggcgg
     1501 aacaacaacc ggttccggaa ggcctggatt gggatctctg gctcggaccg gcgccgtcac
     1561 gcccttacaa caaggcgtat cttcccttcg tctggcgcgg ctggtacgat ttcggctgcg
     1621 gttctttcgg cgacatgggc tgctacagct tcgcgggtct ttttaaaatt ctcgatctta
     1681 cgccccctac ggctgtcgaa gcgtgctcca gcgagtcttt cgaggagacc tatccgaagg
     1741 cgtcgatcgt tcatctggat tttccggcca agggcagccg gcccgcgctg cgtctttcct
     1801 ggtacgacgg cggactcaaa ccgccgcggc ccgcgggcct gagccaggaa gatcaggcga
     1861 tgttcgaccg cgggcgcgag ggcattatgt acgtcggcga taagggtatt ctgctgggcg
     1921 ggttcaacgg tgacaatccg cgcgtgtatc cggaatcgcc gaaatacaaa ttgccaccaa
     1981 gagaacgcgg tgcaagggag cgcgatcgcg ccacggatca gtggctggcg gcggccaaag
     2041 gcggtccggc gccgctcacg aatttcgaga ttcaggcgcc ggcaacggaa gcgttcctgc
     2101 tgggatgcat ggcacagcgg ctggccggca gccggctcga atgggacacg gcgaagatgc
     2161 gcgtcacgaa tctggagaag gcgaatccat acgtcgatcc gccctatcga agtggttacg
     2221 acgtgtgaac ggccgcgtga tctgtggccg gtacgtgtag cggcttgctg gcgggagcct
     2281 cgctgaacca ggccgccatg tattcatccg tgacggtgca ccgcttcatg atctcgcgcc
     2341 tcttcgcgag cgcgcgtgga aacaggcacg ccagccgcca gaatgccggc aaacaggcgg
     2401 gctccgccaa caggcaaccg cccgtcacca gaacgtcgcg catggttatc gacttccaat
     2461 ggcggtggta caggtcgccg gtcatatttt tgatgcgcat caaaaaccgg ttcttcaccg
     2521 agtgcatgcg cagcaccggc ggagcgctgc gccggttggc ggcgctgatc cgccggacgt
     2581 ggcaacccat cgccgatggc acgtacaggc accgccagcc cattaattgc gcgcgccagg
     2641 caatgtcggc gtcttcgcgg taggtgaaaa aatcgggatc gaagaagtcg ccgtcgagcg
     2701 atacatcctc gatcatttcg cggcggtaaa gtgcggccgc ggcggtggca ccgaaaacat
     2761 actccggtgt gttgaatcgg ccgtcgtccg gttcgttcca gccccggtcg aagtgccgtt
     2821 ggctgggcgt gaagtgaatg cccgtagagt cgatgcgccg ctcgcgggga acgctaagat
     2881 cgggcgcggc ccgaagcagt ttgccacaga ccgtgcccac ccctgagccg ctcgatgcgg
     2941 cttccaccag tcgttcgata aaaccgggaa acagccggac gtccggatta agggtcagga
     3001 cccattcgcc atgcccggct gcgatcgcct ggttctgtgc tgcggcaaac cctgtatttt
     3061 catagttgta aagcacacgt accagatctt cgtactccat cagcaactcc gcggtaccat
     3121 cggtcgacgc attatcgacg acgatgacct cgatcggccg gtagtcctgt tcgtagatgg
     3181 aatccaggca ccgttcgata tacttgcggc tgtcaaaggt gacgatggtt acggttacca
     3241 gtggatttgc gaaagccggc tttcccgtca ttgcttataa agaccggaaa agccggcttt
     3301 caactacacc tggagaaaat ttgttctccg gtgtagttgg aagaccgcgc ggagggtctt
     3361 tgaagcaggc tcgttgaaag atgttgtccg actccattca ccgggaagcc ctcgggatcg
     3421 cccgatcgga agggagcgct acgccgcttc aagaccgtgt agcgcggctc ttcgaggcat
     3481 ggcgggagga cgtctaccgt tacctgttga cactgggatt gtatccgccg caggcgcagg
     3541 agacgacgca ggaagttttc ctgaggctct atgtcgtcct gcggcaaaat cagaacatcg
     3601 agaatccacg cgcctgggtt ttccgggtgg cgcataatct cgggcttaaa atacgctctg
     3661 aagagagctg gcagcagccg ttcgacccgg ccatggagga gcggatcgcc gccggtgcga
     3721 cacccgaaaa aagcctgctc cagaaggaac gcgcgctgcg ttttcgccag gctgtcgaga
     3781 gtctgtcaga gcgccagaaa cggtgtctgt tccttcgcct ggaagggttg cgctacgcgg
     3841 aaatcggcgc cgtgctcggc atcagtgctt ctacggtcgg ggaattcatg cgacgcgcca
     3901 tcgcaaagct gagggcaagc aatgaatagc tttgaggcgg gacacccggc agacggcgat
     3961 ttgctgtgtt acctggacgg cgaactccag ggccgtgacg tgaaacgcat ggaggatcac
     4021 cttgcggcgt gctggcagtg ccggacggaa ttgcaggaac tccagcacac gattgccgag
     4081 tgtgtgcaat accggaagac cgtaaactcg agcctgccgg agccgccggc accctggttc
     4141 gatatttacc gccgttttga ccagatcgat acggtaggta cgccggtgat ggtccggttg
     4201 tggaacgtgt tccgccccat cgtggtccgt ccgcaaccgt gggcggcggc tgccgttctg
     4261 gcattggctg tctgggcggc ggtggatcac ttccgtcgcg aagccgagcc cactccggcg
     4321 aagcctgctc ccgtcgggac cgtgcgggct ccacttcctg taacggaagc gccttcagtg
     4381 ccggtgcctc aggcacagcg cgtggagacc ccccggagag aaatcgagca gccgcccaca
     4441 cccggcgatg agttgcgcgt cctggcggtt ctccgccggc tggatgcgga cttgggtgaa
     4501 ccggtggagg taacgcgcgg cagcgagcgc gtgctggtgg cgggcattgg cgttgcccct
     4561 cacttgcagc ggcaaatcga gcaggagctt gagggaatgc ctcgcgtgat ggtgcacttt
     4621 tccgaaccgg cagcggtaat gccgccgtat gtagccggca ctcctggcgc agcggcgcgg
     4681 cgtacggatc tcgcggcgct cgaatcgcga atcgaaaccc atctcggcgg gcgaggggct
     4741 tatgagcagt tcgcgagcga cgtactcgat acgagcgacg cggcgatgtc gcgagtgcat
     4801 gcgctacacc gcctggccga acagtttccg cccgaaatcg agtcgaagat gaccccggac
     4861 gagcgggcgc tgttgacggg tttgcggctg gaacacgcgg ccgctctccg ggatgtttcc
     4921 gccgctctca acgcgcgcgc gatgccggtg ctatctctgt ttggcggaca gccgctaccg
     4981 caccaggcgc cggctccggc actctcctgg caggcggccg ccgaacaact gtttcgagag
     5041 gcccgcaaca tggaatcgct attaggtgcg atgctcggcg gggccgccac gcactcttcc
     5101 gcggcggatc tccctgtgcg gattctttcg agcctcgccg ctgttcacgc tcatgcacaa
     5161 tttcagatcg ggaccgactc cccagccggc atgacctcaa tgccgtctgg ccacaccgga
     5221 caggggcgct cgaagcgggc gccccgccgc gtctcgaaaa gcatagaata gagcccatgc
     5281 ggacgagccg gcggcattgt gatcgcctga tcccgctctg tatcctcttc gcaacgttat
     5341 taagcgcaca gcagcaaacg catatctccg gttggattcg cgatccgtcg ggagccgggg
     5401 tgccggccgc atcgatttcg gtggtggacg aacagacggg attccgccgc accacgtact
     5461 cgcagatgag tggtgcttat gcggttgctt ccctccgccc gggcacgtat aagatcacag
     5521 tccgcaagca gggatttcga acgttgatcc ggtttggagc cgagttggtg gccgctcagc
     5581 ttacacagat ggatttcgac ctgtccctgg gcagcatgca ggaggtgatt accgttcacg
     5641 gttcgcctcc cctgttgaat aatgagagcg tttccgttgg aaccctcatt cgccgggaga
     5701 cgattgacgc gctcccgctg aacggccggg ggctgttgag cctgctcgag cttgcgccgg
     5761 gtgccatcgc gacccctgcg actcgcggcg aggcgggtca gttcagcgcc agcgggcaga
     5821 gaccgaatgc caactatttc accctggacg gtgtcagcgc caatacaggc gtaagcggcg
     5881 gcgggctccc cgcgcaatgt accggagggt cgctgcccgg catgacggcc ctcggcagcc
     5941 tccatggcct tgtgtcgctc gaagcgctgg acgaatttcg tattgagacc tcctcggcgg
     6001 cgcctgaatc cggcacgctg cccggcgccc gcgtgtcgct gagcacccgg tccggcacaa
     6061 atcaatttca tggctctctg tttcattact tccgcgatga gcacctggat gcgaacgggt
     6121 ggttcgcaaa tcaggccggc gagccaagag cacagttgaa tatgtatgac ctgggtgcgt
     6181 ccctgggtgg tccgctgaag cgcaaccgga gttttttctt cgtgtcgtat gagggcatgc
     6241 ggctgcgtgg gccgtttgcc tggcgcgccc cggtgccgtc ggccgaagcg cgtaacaatg
     6301 cgccggagtg ggcgcgtcct gtgctcggat tgctgccgct cccgaacggc aggtggctgg
     6361 atccgaacac cgcggaatgg gccggcagca actaccgccg ctcgcgattc gatgtagcgg
     6421 gacttcgcct cgatcacgcc ttcacatcgc gcctgacggc gtttgccagg ttcaactatg
     6481 caccgtcatc gaaccaattc accggcacac aggtgaacga cctccagatg cgaagcagga
     6541 gcgtcaccgt gggcatgaat ctgcggttga atacggctgc catcctggac ctgaaagcga
     6601 acgtatccga tgcgaaagcg gaatcgttct ggagagccgc ggaatccgcc ggcgactcct
     6661 cctgtttcat tggaccggta accgaattct tcgtgcggac ggaaggactc tgcaattacc
     6721 tgctgcggtt ttcgatttct ggtatagggc aggcgttgtg gggccgcgag gcggaccaga
     6781 ggcagacaca atggcaggtt ttgccttcag ccatattggt tctcggcagc catcagttca
     6841 gaatcgggtt cgaccggcgc cggttcgcgc ctatccggcg cgacaataac ggcagcatca
     6901 gtgtggtcgc cgaggacctt gacgacctgc tcaagagtcg gaatctctgg gtgtctgttg
     6961 ccgccccgag aacgctacga agcgcgatgg aggaaatggc ggcatatgcg caagatacct
     7021 ggcgcgttca tccgcggctg acggccacca tcgggttgcg atgggaatat tcccgccccc
     7081 cgcaacttac tcagggcacc ttttatcagt cgcagggaga ttggcagcat agcggggctt
     7141 acatcttccc ttcgcagagt gagatttgga agcgccgtta caccaatttc gctccacgat
     7201 tcggagtcgc attccagccg ctgggcggca aggcactcgt actgcgcgcc ggtgggggac
     7261 tcttttacga ttccagcctt agtatcgcga cagatctcgt gaacggtggt ccgttcagtg
     7321 tggcgcaata cggcaacccg aggcacgctc cattctcgac gatcatgagc tatggtttcg
     7381 tccccgattt gcgtctgccg gccgtttatc aatggaactt cacagtggaa caggcgctgc
     7441 cgggcaggaa tgtcgtgtcg gcgggttatg tgggcgcttc cgggcatcac ctgttgcggc
     7501 gtgaattcgg cggcgcgggc gcaggtgaaa cgctctggct tgcgcttgcg accaatcaag
     7561 ggcagtcccg ctatcacgca ttgcaggcgc agtaccgccg tcctatggct cacggcttgg
     7621 aggctcttgc ctcttattcg tggtcccact ccatcgacaa cagttcgagc gatgcgctgc
     7681 tgcatcgcac cggtttgaac ctgagcgccg gcggcgaccg gggatcttcc gacttcgatg
     7741 cgcgccatac gttcaccgcc ggtctgacct acgaggtcgg atcgaaatcc ggcgatacgt
     7801 tgtgggcacg tgcgtggagc ggctggggaa tcgacggcat cctgcgcgcc cggtcggcgt
     7861 tcccgattac ggtgctgaat tcggagtatt ccatgggcct gagcttcgcg aacgccttcc
     7921 ggcccgacct ggttggcggg cagccggtct ggattcggga cccttccgcg cccggcagac
     7981 gccgcttgaa ccgggacgcg ttccaactca ctgacgagac cgtacaaggt tcccttggcc
     8041 gcaatgcgat ccggggattc gggatgcagc agatcgacac ggcggtgcgc aggcagttcc
     8101 aactcggcga gcggtggtcc ctgcaattac gcatcgaagc attcaacctc ctaaaccatc
     8161 cgaacttttc cgatccggtg ccgtacctcg gcaatcccct gtttggagaa tccgcttcga
     8221 tgctgaacct gatgctgggg acgggcagcc ccggcagcgg tctgactcct gtgtttcaga
     8281 ccggcggcgc gcgctcgatg cagatcgcac tccgcttgcg gttttgacgg ttgtatgttg
     8341 cgccggaatt ttctcgcctg gccggctgcc gctgttgcgt ggccccaacc atctcgggga
     8401 gcggcggtgg atccgaaggg ttggtccgcc gctcacgtcc ttccggctcc ggacgacgcc
     8461 gctcaatggc ccgcgtgccg tgacgcgctt gcacgatggc gagtggacga gaagagaagg
     8521 atcggctacc gggatgattt gtaccgccgg cccgacttct cgtgggtgcc gtcttctttc
     8581 gcctgctgtt ttctgatgct gtgcgacgag aagttctacg actatcgaag cggccgttac
     8641 gaagtggatg cctggatcga tgtgggcatc cgcgagttcg gaggttacga ttcggtggtg
     8701 ttgtggcacg cctatccgcg catcgggctg gaccagcgca accagttcga tttctaccgc
     8761 gacatgccgg gcggactgga gggagtgcgg agggtcagcc aggctttcca ggctcgcggc
     8821 atcaaagtct acatcaatta caatccctgg gacacgggca cgaggcgcga gggcgtctcc
     8881 gatatcgatg ccgttgtcgc gatggtgaaa gcagtggacg ccgatggcat ctttctggac
     8941 accatgaacc agggctcctc ggagtttcgg gtgaaactgg acgaggcgcg ccggggcgtg
     9001 gtgctcgaaa gtgaaggcgc cctgccctca ctcgaaaata tccaggatca ccatatgtcc
     9061 tgggcgcaag gctttgagga cagcgccgtg cccggtgtcc tgcgcgataa atggttcgag
     9121 cggcgccaca tgcagcacca gatcaagcgg tggaatcgcg atcacaccgg cgagttgcac
     9181 gcggcctgga tgaacggcag cggcatcatg atttgggaga atgtcttcgg aag
//
LOCUS       DASTMK010000106         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_453185, whole genome shotgun sequence.
ACCESSION   DASTMK010000106 DASTMK010000000
VERSION     DASTMK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453185"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFQ79_04270"
     CDS             <1..245
                     /locus_tag="VFQ79_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138897.1"
                     /translation="MGGHPMGPLLYFGFYFDLPPITQIVTKAQRVLGFRATDFAAGLK
                     ETYRWYVRNQERKPLDYSFEDSLLAGENVTFGTGQL"
     gene            268..1581
                     /locus_tag="VFQ79_04275"
     CDS             268..1581
                     /locus_tag="VFQ79_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0138898.1"
                     /translation="MKRRNFLAALSVAAAASAQNEPEAPRVTHARATFGDRIEPDWEQ
                     RVSISVGPEKADLVGSDHRAIQAAVDYVAGLGGGTVQILPGTYKLRNAVYLRSGVRIS
                     GSGLDSVLIKEPSVKTALAENSDWYDMEVTLADASGFKVGDGVVLRTKNPHNGSHDTY
                     KRTLVARSGNRFKLDQMLVENYWVKGGATAATLFAIFDGYRISDVSIENICLDGNRAN
                     NDFLNGNYVGCVFVRESNNIRFRKVTARNFNGDGISWQVCHDVRVEDCHSHDNAGFGL
                     HPGSGSQRSVVTGSRLDQNDIGFFFCWGAKWSLVEKNTMTGNKRFGISVGHNDTDNII
                     RHNEVLRSGKFGVYFRQEHGPTFSPNRNRLEDNRIADSGAEDGVAVEVEGQTQSVAIA
                     RNTLVETRQPASRIGVRIGRETKNITLAGNRIDGFSVPVQDLRQT"
     gene            1602..2618
                     /locus_tag="VFQ79_04280"
     CDS             1602..2618
                     /locus_tag="VFQ79_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HEU0138899.1"
                     /translation="MAFAAALIAIAAVANTKDVLSGCFQWEASAPLISPEDRDGDHYY
                     SIKDPSVVQYEGQWHLFCTIRGQKRSHQIEYVSFRDWSAIARARRDTLRLTDGYFCAP
                     QVFFFEPQKKWYLVYQVVDESRTPALQPAFSTSTDIADPSSWTPPALLLSGSSPGVKD
                     WIDFWVVCDNRKAHLFFTTLDGRMWRSEAPLSEFPNGWQQPRVVLEDDIYEASHTYRL
                     KGLDNYLTIVEARAPAGRRYYKAYLADALDGAWTPLAASADRAFAWPGNVRFPKEAWT
                     DSFSHGELLRDGYDQTLTVDPEKLKFLFQGVSDANRQGKIYGQIPWSLGLLSPVPEGC
                     RTPF"
     gene            2629..>3846
                     /locus_tag="VFQ79_04285"
     CDS             2629..>3846
                     /locus_tag="VFQ79_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU0138900.1"
                     /translation="MITGSEHQRDLERVLRIMEDTPAIDRRYMAPREEFEGRIRKVNA
                     ALEREGHTAGFVFSDEHYRGDVPYLGGNTNVSIEQVAGVIGPNGFHLVAGLEGGYVAE
                     QLAGRAATAVHKVEMLQLADEKYPIRAERLEDVIEAAAGGKVDHIALLTPRQVVPAAM
                     VDYLENLFGREGVVDAQELYYRAKYEKSDIEMRLIKDACTIADAMLRSMLAVLRPGRL
                     ETEVAAWGAWVGRMLGSESDGFRIMVGANEANRTLIGPALNRPIGEGEWVHLGVAPQR
                     DGLTSCVRRSVIAVDDPSKITDEQKFWLSLVEEGYRVGEEAYRKVAAENLPARLQEQA
                     LVDYFASRADEVSRRIGRKIDLASLKPYTGTHNSGYTECQEFFGAITLDSHEPLGSQS
                     VTMLDVALRGIGDH"
BASE COUNT          787 a         1111 c         1204 g          744 t
ORIGIN      
        1 agatgggtgg gcaccccatg ggtcctttgc tttatttcgg cttttacttc gatctgcctc
       61 ccatcaccca gattgtgacg aaagcacagc gggttttggg cttccgcgcc acggactttg
      121 ccgccggcct gaaggagacc tatcgctggt atgtccggaa tcaggaacgc aagccgctgg
      181 attacagctt tgaagattcc cttctggccg gagagaacgt cacgttcgga accggtcaac
      241 tataatggcg atattccaga ggtgtgtatg aaaaggcgta attttctcgc tgctctttcg
      301 gtggctgccg cggcgtctgc ccagaatgaa ccggaagcgc cccgcgtgac ccacgctcgc
      361 gctacgtttg gagaccgcat cgagcccgat tgggaacaac gcgtctccat cagtgtcggt
      421 ccggaaaaag ccgacctggt gggttcggac caccgcgcca tccaggccgc cgtggactat
      481 gtcgccggct tgggcggcgg caccgtacag atcctgccgg gaacttacaa gctgcgaaac
      541 gccgtctatc ttcgatccgg cgtgcgcatt tccgggtccg ggctcgattc ggttctgatt
      601 aaggagccgt cggtaaagac ggcgctcgct gaaaattcgg actggtacga catggaagtc
      661 acgctggcgg atgcgtccgg cttcaaagtg ggcgacggcg tcgtgctcag gactaagaat
      721 ccgcataacg gcagccacga tacatacaaa cggacgctgg tggcccgcag cggcaatcgt
      781 ttcaaactcg accagatgct ggtggagaac tattgggtga aaggaggcgc cactgccgcc
      841 acgctgtttg ccatcttcga cggctatcgc atttccgacg tcagcatcga aaacatctgc
      901 ctggacggca atcgcgccaa caacgatttc ctgaacggca attatgtcgg ctgcgtgttc
      961 gtgcgggaga gcaacaatat ccgtttccgc aaggtgaccg ccagaaactt caacggcgac
     1021 ggcatcagtt ggcaggtctg ccacgatgtg cgcgtcgagg attgccacag ccacgacaac
     1081 gccggattcg gcctgcatcc cggctcgggc tcgcagcgct ccgtcgtcac cggaagccgg
     1141 ctggaccaga acgacatcgg cttcttcttc tgctggggag ccaagtggag ccttgtcgaa
     1201 aagaatacga tgaccggcaa caagcgtttc gggatctctg tcggtcataa cgataccgac
     1261 aacatcatcc ggcacaacga ggttcttcgc agcggaaagt tcggtgtgta tttccggcag
     1321 gaacacggac ccacgttttc gcccaaccgg aaccggctgg aggataaccg catcgccgac
     1381 agcggcgccg aggatggagt ggcggttgag gtggaaggcc agacccaatc ggtcgcaatc
     1441 gcccgcaaca cgctcgtcga gacacggcag ccggccagcc gcatcggtgt gagaatcggc
     1501 cgtgagacga agaacatcac attggctggc aaccgcatcg acggattttc cgtaccggtc
     1561 caggacctca ggcaaacctg atgaaaagca ggagtctttc gatggctttt gcggcggcgc
     1621 tcatcgccat cgctgccgta gcaaatacga aagacgtcct gtcggggtgc ttccagtggg
     1681 aagcgtccgc tccattgatt tcgccggagg accgcgacgg cgatcattac tactccatca
     1741 aagacccgtc ggtggtgcag tatgaaggcc agtggcacct gttctgcaca attcgcgggc
     1801 agaagcggtc gcatcagatc gaatacgtct ccttccgcga ctggtccgcc atcgcgcgcg
     1861 cccgccgcga tacgctccgg ctgactgatg gctatttctg tgcgccgcag gtgttctttt
     1921 tcgagccgca gaaaaagtgg tacctcgtct accaggtagt agatgagtcg cggacaccgg
     1981 ccttgcagcc tgccttctcc accagcaccg atatcgccga cccgtcttcc tggacaccgc
     2041 ctgcgctgct gctctccggc tcgtcgccgg gcgtgaagga ctggatcgat ttctgggtgg
     2101 tgtgcgacaa caggaaggcc cacctgttct ttaccaccct ggacggcaga atgtggcggt
     2161 cggaggctcc gctatccgag tttcccaacg gctggcagca gccgcgcgtg gttctcgagg
     2221 atgacatcta cgaggcatcg cacacgtacc gcctgaaagg gctcgacaat tacttgacca
     2281 ttgtggaagc gcgggcgccg gcggggcgcc gctactacaa ggcttacctt gcggacgctc
     2341 tcgatggcgc atggacgccg cttgccgcat cggctgatcg tgcgttcgcc tggcctggca
     2401 acgtcaggtt tccgaaagag gcgtggacag actcgttcag ccatggtgaa ctgctgcgcg
     2461 atggatacga tcagacgctc acagtggacc cggaaaagct gaagtttcta tttcagggcg
     2521 tgagcgacgc aaatcgccag ggtaagattt acggccagat tccatggagt ctcgggctgc
     2581 tcagtccggt tccggaaggc tgccgcacac cgttttaggg aggatagatt gatcacaggt
     2641 tcggaacatc agcgcgatct ggagcgggtc ctgcgaatta tggaagacac gccggcgatc
     2701 gaccgtcggt atatggcgcc ccgcgaggag tttgaagggc gcatccggaa agtgaacgcg
     2761 gccctcgagc gcgagggcca caccgccggg ttcgttttct ccgacgagca ctaccgcgga
     2821 gacgtgccgt accttggcgg caacaccaac gtttccatcg agcaggtagc gggcgtcatc
     2881 ggtccaaacg gcttccatct cgtagcgggg ctggaaggcg ggtacgtcgc cgagcaactc
     2941 gccgggcgcg ccgccaccgc ggtccacaaa gtggagatgc tgcaattggc cgatgagaaa
     3001 tatccgatcc gcgcggaacg gctggaggat gttattgaag cggctgcggg tggaaaagta
     3061 gatcacatcg cgctgctgac accgcgtcag gtggtgcctg cggccatggt tgattacctg
     3121 gaaaacctgt tcggacgtga aggtgtggtg gatgcgcagg aattgtacta ccgcgccaag
     3181 tacgaaaaga gcgatatcga aatgcggttg atcaaggacg catgcacaat cgccgatgcc
     3241 atgcttcgca gtatgctcgc ggtcttgcgt cccggacgcc tggaaacgga agtggccgcc
     3301 tggggcgcct gggtgggccg gatgttgggc tccgagagcg acgggttccg cattatggta
     3361 ggcgcgaatg aggccaaccg gacgctaatc ggcccggcgt tgaaccgccc catcggagaa
     3421 ggagaatggg tgcacctcgg cgttgcgccg cagcgggacg ggctcacttc ctgcgttcgc
     3481 cgctcggtga tcgccgtgga cgatccctcg aagatcacgg acgaacagaa attctggtta
     3541 tcgctggtgg aggaaggtta tcgcgtcggc gaagaagcgt accgcaaggt cgcggcggag
     3601 aacctgccgg cccggttgca ggaacaggcg cttgtagatt attttgccag ccgcgccgac
     3661 gaggtatccc gccgtattgg gcggaaaatc gatttggctt cgctcaagcc gtacacaggc
     3721 acccacaact ccggatacac cgagtgccag gaatttttcg gagcgatcac gcttgacagc
     3781 cacgagccgc tgggcagcca gagtgtgacg atgcttgacg tagcgttgcg cggcatcggc
     3841 gatcat
//
LOCUS       DASTMK010000107        15670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_101846, whole genome shotgun sequence.
ACCESSION   DASTMK010000107 DASTMK010000000
VERSION     DASTMK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15670
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101846"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFQ79_04290"
     CDS             <1..400
                     /locus_tag="VFQ79_04290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEU0138901.1"
                     /translation="GGWLRPFPNVGFLEVPLNYVFPALLMVGSGVGSFLLLKNLKSGA
                     QKAVLAAIAVVLMATGLLVLIPAVNQALIGLFWFFLKVAVMVYALIWFRGTFPRFRYD
                     QLMNIGWKYMIPIGMGAILVNAVVGIALAK"
     gene            614..1186
                     /locus_tag="VFQ79_04295"
     CDS             614..1186
                     /locus_tag="VFQ79_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0138902.1"
                     /translation="MLNAKVLVVVVGSLLSPVWLGAAQTQKKEMTADRVSERGMNRIE
                     RQVRHELVMLPYYGVFDNFMFRVDGSTVTLMGQVRRPTLKSSAERVVQDIEGVENVVN
                     NIEVLPTSPNDDRIRMAAYRAIYGHTALNRYAMRAVPPIHIIVKNGDITLEGVVANES
                     DKNIAYMQAQSVSGAFKVTNNLRVEKGGEE"
     gene            complement(1209..1793)
                     /locus_tag="VFQ79_04300"
     CDS             complement(1209..1793)
                     /locus_tag="VFQ79_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138903.1"
                     /translation="MRPLQIFCMGFVLTTYGLPAQELRYEVESQENRPLALSVTPHTD
                     DREPYLTIQLTNVSKKEVVAYGLLVSSRKATGGTRPVQYIQRRKLAAASTAKQPFSSG
                     KTFKPHTVTPITDRAGKPLPLVITLDYVLFADGSKWGPDQEIRGAEMAAYLWGSEEIL
                     RTLKGIRNQEGLTEVTVLDQVEAPANCDSCSRKK"
     gene            complement(1871..3484)
                     /locus_tag="VFQ79_04305"
     CDS             complement(1871..3484)
                     /locus_tag="VFQ79_04305"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HEU0138904.1"
                     /translation="MDLPSRIGKYELLEYLGGGMARVYKARDTVIGRVVALKLLTESG
                     AADPEAKARFLQEVKMAGNVTHENVISIFDYGEEQGRPFIVMEFLTGTDLRNAIRDGQ
                     TGSLEDRLRIALQVARALEYTHSKKIIHRDIKPENINLDPGGRAKLMDFGIAKSQDLT
                     LTKTGFALGTPYYMAPEQVLGKPATELVDVYAFGILLYELLTGVKPVQGDTIERLFYI
                     ILHEPINMQPLADANIPERIRNVIARCTAKDPSQRIQSFREIRQELEDIAEPPKPAAG
                     GGKPRRRSLILGALILVAASLFGIFLALRQRAPVEQGLPATLSTPTGEMVLVPAGAFL
                     FGEDRTSVNLPAYYIDRTEVTNAVWAEFCRQTGRRPPASFPGDRPDYPVVNISFHDAQ
                     AFAAWAGKRLPTMQEWEKAARGTDGRDFPWGSTADPQLANVSDNTALAVKGIMPATAF
                     PPGASPFTALNMAGNVWELVDDARAPSARAIESFANLSPPPTAGEPWRIIRGGAWDVP
                     LRNCVTYEWASVPARLTQPNIGFRCVKNP"
     gene            complement(3490..4506)
                     /locus_tag="VFQ79_04310"
     CDS             complement(3490..4506)
                     /locus_tag="VFQ79_04310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0138905.1"
                     /translation="MQLPARIGKYELEEFLGGSMSNVYRARDIVLGRSVVLKILTEAG
                     CADPEAKARFLQEARLASNIVHENIISVYDFGEERGQPFIVMEYLRGDNLRDLIRQGR
                     TGDYRKKLNMALQIARALGYIHSHRIVHRDIKPENIHIDAAAGKIKLMDFGIAKSEGV
                     QLTRAGFTLGTPYYMAPEQVTGERITHLVDVYAFGVLLFELMTGVKPVTGDSVDQIFG
                     RILQEPIRQEPLRAAGVPQHVIELIARCTEKLPAARIQSFNEICALLEEMVAVPAAPP
                     IRPVGKAPAVPARSAPHAGTMPGLLAKLPARFQTQNWIVVLVAIAVLLVMSASYLIAR
                     ALIR"
     gene            complement(4525..8013)
                     /locus_tag="VFQ79_04315"
     CDS             complement(4525..8013)
                     /locus_tag="VFQ79_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HEU0138906.1"
                     /translation="MSSQLATAAAYAGYLVLVWTVGRLLGLTGASYILFGLVLSVLGA
                     LAAGAWLWFTGKFRKTSPAQDSVPDSTAASGGSDELDLLFREAGNRLRSARIVQESDI
                     RNVPAIVVIGGPGSAKTSTVLHCGLEPELLAGHVYQDNAVTPTRTANFWLSGRTVFVE
                     AGGRLLGETSRWLQFIKRLQPGKLAAAFKSGGQAPRAVVVCLDSEIFTKPGASEAVAS
                     MARNLHARLGDISQTLGISFPVYVLFTRADRLPFFLDYVRNLANEESGQVAGTTLTIR
                     PPQSEGVYAEEETRRLTAAFERLFHSLCDKRINFLARENDPAKLPGTYEFPREFRKIR
                     TLAVQFLVDLCRPSQLRASPFLRGFYFSGVRPVLVSESAPVPAIPSQPERQMQAPATA
                     TQAFRVGAQQAPPQPAAQPVSGTRRVPQWLFLTHLFNDVILQDRAAMGASSASVKSSN
                     TRRFLLAAASVAFLLLGIAFTVSFIGNRALENEVIEAARNLGTPAAASDTLPSVESLR
                     ELETLRQAYARLAMYEREGPPLRLRWGLYAGDDMYPHARRIYYDRFKRLLFGHTQAGM
                     AQWLAQRPASPGANDDFGYAYDTLKAYLLTTYEHKRTVPSFLSPVLMNRWLAGRELDP
                     ERVDLARRQFDFYASELAAQNPYSTEADAGAVDRSRRYLAQFGGTESIYQFMLTESAR
                     NNPKVNFNEQFPGSAQVLVNNKDVPGAFTRGGWKFMQDALSKVDKFFGGEKWVLGDYA
                     SADIDRIKIEKDLRERYTNDYIARWREYLRNSTVLRYSNLKDAARKLNMHSSNQAPVL
                     ELLWLATQNTTVDSPRVSQAFDAVHKVVPPPATTPQFVLPPNSNYVSALAGLQVAIEQ
                     AASQPVPNPAAVQQALSNATTAKLASKQIAATFLVDQEGKVHAMVQKLMEDPITQAEG
                     LLQGLGPAELNGKAKGFCAIYGTLMRKYPFDSESKQQATLEEVNAIFQPKQGALWKFY
                     ESDLQQLLTWQGTQFVPAPPSPGSVELNPQFVAFLNRAAAFSAALYPAGSPAPQLHYT
                     LAPQPTEGIQALTLMIDGQTLTAGPRGGSPVKFTWPGTGAPQARMTGKLGGPDVQLLN
                     YAGTWAAFQLFGEAQWQPSGAGYNLEWIPRTSGQPMMVNNKPLTIRFFLDMGGAPPFF
                     RKGYFAGMQCVARAAR"
     gene            complement(8017..8814)
                     /locus_tag="VFQ79_04320"
     CDS             complement(8017..8814)
                     /locus_tag="VFQ79_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HEU0138907.1"
                     /translation="MTENARRAENLALAFQEVLTAIVRLRSNRQTVSDAAAFRGQIRE
                     AIRMADQEARRAGYSAEDVEDAKFAVVAFLDESILNLRSPVFTDWPRRPLQEEFYGHH
                     IAGEVFFRNLQRLLVRDESRDVADVLEVYHLCMLLGFAGRYSIGGRGELQGITGMVAA
                     KIQRIRQTPSQLSPGWALPGDSIQVTRGDPWLKRAFIAAICCVSLAVALFIVYMLLLR
                     SGVGTIRGIGAGHGSEMEPRALASGITCFRFDCAKPLPRGRGSETGT"
     gene            complement(8890..10245)
                     /gene="tssK"
                     /locus_tag="VFQ79_04325"
     CDS             complement(8890..10245)
                     /gene="tssK"
                     /locus_tag="VFQ79_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEU0138908.1"
                     /translation="MKFLSKVVWSEGMYLGPHHFQVQNRYFEDSIRFATSSVWFEPHG
                     FTGYQLDAEALRNGTVGLIHARGILPDGLLFQMPESDPLPAVRAIADLFPPTRDFLTV
                     LLAVPTRKQDGMNCAAGDSEFAMEARYVAENQVVHDDNTGREEKPVQLGRKNIRFLLD
                     TEDTEGMLTMPLARVLRDGAGHFIYDPEFIPACVRIGASEPLMLMLQRLVEILEDKST
                     ALSRGRKSGAGAWAEYSTRDIASFWMLHAVNSAIAPLRHQLMTKRGHPEELYVEMARL
                     GGALCTFAIDSHPRAIPLYDHNELGRCFQELDRHIRKHLEAIVPTNCLRISLEKTADY
                     FYAGQITDQRVLDRSRWVLAIGSPAGEVDLITKTPQIVKVCSEKYVAELVKRALPGLA
                     LTHLPVPPSTVSAQVDMQYFSVDKVGPCWDSIVKTREIGIYVPGDLPDTQLELLVVLE
                     S"
     gene            10474..12003
                     /locus_tag="VFQ79_04330"
     CDS             10474..12003
                     /locus_tag="VFQ79_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138909.1"
                     /translation="MSFYKKYELLELVRDDGIKTFAAREIRTGMPVQVHLFTGVSPAE
                     SDLLWSQLDAIPLERRRGLFAYAEYEGTRYVVTEPLTSPENLRTWVGARTTPAPAPPP
                     ATEDVLVKAGSWKIPSALLEKKPPEPEGQPAPTERMNIEEPPAAAPPPEPEPGEFTRM
                     FRSPPEPERPAAPTERMNVTEPPAAAPPPEPEPGEFTRMFQSPPEPPVSQPAKPAPPP
                     EPGEFTRMFAPQPLPKPEPQKPPPAQVQIRKLSPEEQGLAPEGKPRPVFLTPERPASA
                     KPEPAPPAQSEPDEFTQMFQAPESPQEEPGDFTRMFSAPPAEPEPQPSPEESGPGEFT
                     RMFQSPPQPEKPRQPPPPPGEPGEFTRFFENAPRQAPWPSSASQGTPPPPSHPGPLDQ
                     PGEFTRMFGGDVTLKQPAPREPAPPYGGATQSFSPPSGPHPPSVPSTGSRGAPGEYTR
                     MIERPREFSAEPQPPQPFNLGEQRAGAAQKPSYLPLILIFGVLFLLAVAVVLFFVLRK
                     P"
     gene            complement(12008..13363)
                     /gene="tssK"
                     /locus_tag="VFQ79_04335"
     CDS             complement(12008..13363)
                     /gene="tssK"
                     /locus_tag="VFQ79_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEU0138910.1"
                     /translation="MKSLQPVIWMKGTFLTPQYLQAQDRFIESSLEFQLHALAFRPWG
                     FRTLRIDQEALAAGMFAVSASSGILPDGLLFDIPDSDAAPPVKPIAEAFEQDRNELDV
                     YLAIPDYRERGLNIALANHGADTRFLAEVALLRDENTGMNERPVQVARKNFRILVEGE
                     SRQGCSALHAGRVRKTGAGTLQLDPRFVPPLLDITASEYLVSILRRLVEILSAKSTML
                     AGTRRQKNLTLADFGTADIANFWLLYTINSHFPLLQHLFEVRRGHPETLYSAMLSLAG
                     SLTTFSTSVHPRDLPAYDHDDLSLCFTSLDEKLRFLLDTVVPSNFVSLPLKLVQPHIY
                     ATSLAEDRYLSNTRMYLAVSAPINEGELIGRAPHLIKVCSANHIEHLVRQALPGVQMV
                     HTPRPPSAIPVKLNHQYFSLSQTGPAWEAITRARNLAAYVPGDFPEPQLELIILLPQA
                     A"
     gene            13730..14260
                     /gene="tssB"
                     /locus_tag="VFQ79_04340"
     CDS             13730..14260
                     /gene="tssB"
                     /locus_tag="VFQ79_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEU0138911.1"
                     /translation="MARESTQHKLDRVRPPRVHVTYDVEIGDAIEMKELPFVMGVLGD
                     FTGQAETPLPRLRDRKFIEVTPDNFDSVLEGMKPHLAFSVENKLSEDPDAGNIKVDLR
                     FKSLDDFEPENVARQVKPLRELLDLRTKLTDLRGSLQGNDKLEELLLEAVSNTEKLDK
                     LKGEMTPGEPKETGNE"
     gene            14253..>15670
                     /gene="tssC"
                     /locus_tag="VFQ79_04345"
     CDS             14253..>15670
                     /gene="tssC"
                     /locus_tag="VFQ79_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEU0138912.1"
                     /translation="MSEPQKSMQQGAVEQTAEVSLLDQIVEQGRLARDESARERGKDM
                     VKEFVQQVLEGSMTVSKDAETMINARIAQIDHLLSIQLNEILHHPSFQKLEGTWRGLK
                     YLLDQSETGTHLKIKVLNASKKELLRDLQRAAEFDQSALFKKVYEEEFGVFGGAPFGA
                     MIGDYEFGKGPEDIELLERISQVAGAAHAPFMAAAQPEFFNLDKFTSLDAPRDLAKVF
                     DTTEYAKWKSFRQSEDSRYVALCVPHILMRLPYGKDTKPVDAFSYEEAVDGTDHSKYL
                     WGNAAFALGARITNAFALYGWCAAIRGVEGGGLVEGLPVHNFYTDEGDIAMKCPTEVP
                     ITDRREKELADLGFAPLVHCKGTDYAAFFSVQSAQKPKLYDLESANANARLSAQLPYI
                     LAVSRFAHYLKVMMRDKIGSFMSRGDCERWLNQWIQNYVTPDDQASPSVKAERPLREA
                     SIQVAEVAGKPGVYKAVAFLRP"
BASE COUNT         3218 a         4554 c         4813 g         3085 t
ORIGIN      
        1 gggcgggtgg ttgcggccct tcccgaatgt cggattcctg gaagtgccgc tgaactatgt
       61 attcccggcg ctgttgatgg tgggctccgg cgtgggatcg tttctgctgc tcaaaaacct
      121 gaaaagcggc gctcaaaagg ccgtgcttgc cgccattgcg gtagtgctga tggcaaccgg
      181 actgcttgtc ctgattccgg ccgtgaacca ggctttgatc ggattgttct ggttcttcct
      241 taaagtcgct gtgatggttt atgccctgat ctggtttcgg ggcacctttc cgcgctttcg
      301 atacgatcaa ctgatgaata tcggctggaa atatatgatt cccattggaa tgggcgccat
      361 attggtgaac gcggtagtgg gaatcgcact cgccaaatag ttctgttttt ttaaggaacc
      421 tatcgccgta caatttcgta cgtcacatat acaaaccggg attacgggct ccgtgcccct
      481 atctccccaa acaccgagac tgcacggagc cccccggctc gggatattcc cgttagttgc
      541 cgcaacagag ttgcctggaa tgcatcttac cttatagaag ctggtgtggt taagccagga
      601 tgagaggtga aagatgctga atgcgaaggt actagtggta gttgtgggta gtttattgtc
      661 gcctgtctgg ctgggagcgg cgcagaccca gaaaaaagag atgaccgcgg accgggtctc
      721 cgaacgcggg atgaaccgca tcgagcggca ggttcgccat gagttggtga tgctgcccta
      781 ctatggcgtg ttcgacaatt tcatgttccg cgtggatggt tccaccgtga ccctgatggg
      841 gcaggtgagg cgtccgacgt tgaaatcgag tgcggaacgc gtggtacagg atatcgaagg
      901 cgtggagaac gtcgtcaaca acatcgaagt tttacctacg tcgccgaatg acgaccgcat
      961 tcgaatggcg gcttaccgcg ccatttacgg ccatacggcc ttaaaccgct acgcaatgcg
     1021 agccgtgccg ccaattcaca tcatcgtgaa gaacggggac attacgctcg aaggcgtggt
     1081 ggcgaacgaa tcggacaaaa acatcgctta catgcaggcg cagagcgtga gcggagcgtt
     1141 caaggtaacc aataaccttc gggtagagaa gggcggggag gagtaacttc cggcccacgc
     1201 gtcgtttttt atttctttcg cgagcaggaa tcgcaattcg caggggcttc cacctgatcc
     1261 agaacggtca cttccgtaag cccctcttga ttcctgatcc ccttcagcgt ccggagaatt
     1321 tcttcggatc cccaaagata agcggccatt tccgccccgc gaatttcctg gtccggcccc
     1381 cattttgacc cgtcagcgaa tagcacataa tccagcgtaa tcaccaaagg gagcggctta
     1441 cctgccctgt cagtgatggg agtaaccgtg tgaggcttaa aggtcttacc agacgaaaag
     1501 ggctgcttgg cagtgcttgc agcggccagc ttccggcgct ggatatactg cacggggcga
     1561 gttccacctg ttgccttccg tgaagagact agaaggccgt aggcgaccac ttcctttttc
     1621 gaaacattcg taagctgaat tgtgaggtat ggctctctgt catcggtgtg tggagttacg
     1681 gacaaagcga gcggacggtt ttcctgggat tccacctcat aacggagttc ctgagcaggc
     1741 aaaccgtaag tcgtcaaaac gaatcccatg caaaagatct gcagcggtct cattccgaaa
     1801 ctcctttcct cgggcgatca gccactattc taaacggatc agcaatcgat acggtatgcg
     1861 gggccacggg tcaaggattt ttgacgcaac ggaatccgat gttcggctgc gtgagacggg
     1921 ccggcaccga cgcccattcg taagtgacgc aattccggag aggcacatcc caggctccac
     1981 ctcgaattat ccgccagggc tcaccggccg ttggcggggg gctcagattc gcgaaactct
     2041 cgatcgctcg cgcactgggc gcccgcgcat catccaccag ttcccagacg tttcccgcca
     2101 tattcagcgc tgtgaacggg ctggcacccg gcggaaaggc ggtcgcgggc ataatgcctt
     2161 taaccgccaa agccgtgttg tcggaaacat tcgccagttg ggggtccgct gtagagcccc
     2221 acgggaagtc gcgcccgtcc gtcccgcgcg ccgctttttc ccattcctgc atggtgggca
     2281 gacgcttccc cgcccaggcg gcgaacgcct gggcgtcatg aaaactgatg ttcacaaccg
     2341 ggtaatccgg ccggtctccc gggaaactcg ccggcggacg ccgtcctgtt tgccggcaga
     2401 attccgccca gacggcgttc gttacctccg tcctgtcgat atagtaagcg ggcaggttga
     2461 cgctcgtgcg gtcttcgcca aacagaaacg ctccggcggg aaccagaacc atttcgcccg
     2521 taggcgtgga gagcgtcgcg gggagtccct gttccaccgg agcccgctgg cgaagcgcca
     2581 ggaaaatccc gaataacgaa gcggccacca ggatcagggc gccgagaata agcgatctgc
     2641 gccgcggctt cccaccgccg gcggccggct tcggcggttc tgcgatgtct tcgagctcct
     2701 gccggatctc gcgaaagctc tgtatccgtt gggacgggtc cttcgccgtg cagcgtgcga
     2761 tgacattgcg gatgcgctct ggaatattcg catccgccaa aggctgcatg tttatcggct
     2821 cgtggagaat gatgtaaaac agccgttcga tggtatcgcc ctgtacgggt ttgacaccgg
     2881 tcagtagctc gtataaaaga atgccgaaag cgtagacatc aaccagttcc gtggccggct
     2941 tgcccagtac ctgttcgggc gccatgtagt agggggttcc gagtgcaaat ccggtcttgg
     3001 tcagcgtaag atcctgggat ttcgcgatgc cgaaatccat cagcttggcc cggccgcccg
     3061 gatccaggtt aatgttttca ggcttgatgt cgcgatgaat aatcttcttc gaatgggtgt
     3121 attccagcgc gcgggcgacc tggagcgcaa tgcgaagcct gtcctcgagc gaaccggtct
     3181 ggccgtcgcg aatcgcgttc cggaggtccg tgccggtgag gaattccatc acgatgaagg
     3241 ggcggccctg ttcttcgccg tagtcgaaga tgctgatgac gttctcgtgc gtgacgttgc
     3301 ccgccatctt cacttcctgc aaaaaacgcg ccttcgcctc cgggtccgcc gcgccgctct
     3361 cggtcagaag cttcagcgcc acgacgcgcc cgatgacggt atcgcgtgcc ttgtagacac
     3421 gcgccattcc gccgccgagg tattcgagca attcgtattt gccgatgcgt gagggaaggt
     3481 ccatggaact cagcgaatca atgcgcgcgc gatcaggtag gaggcgctca tcaccagaag
     3541 gaccgcgatg gcaaccagaa ctacgatcca gttctgcgtc tgaaatcgtg ccggcaattt
     3601 cgccaggagg ccgggcattg tgccggcgtg cggggcggag cgggccggca ccgccggtgc
     3661 ttttcccacc gggcggatgg gaggagccgc gggaacggcg accatttctt cgagcagagc
     3721 acagatttca ttgaaacttt gtatccgggc ggcaggtagt ttttctgtgc agcgggcgat
     3781 gagttcgatt acgtgctgcg ggacgccggc ggcgcgcaac ggctcctgcc gtatcggttc
     3841 ctgcaaaatc cgtccgaaga tctggtcgac ggagtcgccg gtaaccggct ttactccggt
     3901 catgagttcg aacaggagaa caccgaacgc atagacatcc accaggtggg tgatccgttc
     3961 tcccgtcacc tgctccggcg ccatgtaata cggtgtgccg agagtgaagc ctgcccgggt
     4021 caactgcacg ccttcggact tcgcgatgcc gaaatccatc agcttgattt ttccggcggc
     4081 ggcatcgatg tgaatgtttt cgggcttgat atcgcgatgg acgatccggt gggaatggat
     4141 gtagccgagg gcgcgcgcaa tctgaagcgc catattcagt ttcttgcggt aatcgcccgt
     4201 ccgtccctgg cgaatcaggt cgcggagatt gtcgccacgg aggtactcca tgacaatgaa
     4261 gggctggcct cgctcttcgc caaagtcgta cacgctgatg atattttcgt ggacgatgtt
     4321 gctggccagg cgggcttcct ggagaaatcg ggccttggct tcggggtcgg cgcatcccgc
     4381 ctcggtcaga atcttgagca ccacgctgcg gccgagcacg atgtcgcgcg cacggtagac
     4441 attggacatg ctcccgccca ggaactcctc cagttcgtat tttccaattc gggccggaag
     4501 ctgcatgggt acaggctcag gttatcaccg cgcagctctg gccacgcact gcatgcccgc
     4561 gaagtaacct ttgcggaaga atggcggcgc gcctcccata tcgaggaaga accggatggt
     4621 caacggcttg ttgttcacca tcatgggctg cccgctggta cggggaatcc attccaggtt
     4681 gtagccggcg ccggagggct gccattgcgc ttcgccgaat aactggaatg cggcccacgt
     4741 tccggcgtag ttcagaagtt gaacgtccgg tccacccagt tttcccgtca tcctcgcttg
     4801 tggagccccg gtgccgggcc aggtgaattt gaccggtgag ccgccccgcg gtccggcggt
     4861 cagcgtttgg ccgtcaatca tgagagtaag cgcctggatg ccttccgtgg gttgtggagc
     4921 cagcgtgtag tgcaattgcg gcgcgggcga tccggcggga taaagggctg ccgaaaatgc
     4981 cgcggcgcgg ttcaggaaag cgacgaattg cgggttcagt tcgacgctgc cgggggaggg
     5041 cggcgcgggc acgaactggg taccctgcca ggtcagcaac tgctgcaagt ccgattcgta
     5101 gaacttccac agcgcaccct gtttcggttg aaagattgcg ttcacttcct ccagcgtggc
     5161 ctgctgtttg gattcgctgt cgaacggata tttccgcatc agcgtcccat aaatcgcgca
     5221 gaaccctttt gctttgccat tcaactcggc gggccccaag ccttgtagaa gtccttcggc
     5281 ctgcgtgatg ggatcttcca tcagcttttg aaccatcgca tgcaccttgc cctcctggtc
     5341 caccaggaag gtggctgcaa tctgtttgct tgcgagcttg gccgtggtgg cattgctcaa
     5401 ggcctgttga acggcggcgg gattgggaac cggctggctc gccgcttgtt cgatggctac
     5461 ctgtaatccg gcgagcgccg atacgtagtt cgagttcggc ggcagaacga actggggcgt
     5521 cgtggccgga ggcgggacca ctttgtgcac ggcatcgaat gcctggctca ctcggggtga
     5581 gtccaccgtg gtgttctgcg tggccagcca caacagctcc agaaccggcg cctggttcga
     5641 ggaatgcatg ttcaatttcc tggcggcgtc tttgaggttg ctgtaccgga gaacggtgct
     5701 attccgcagg tattcgcgcc agcgcgcaat atagtcgttc gtgtagcgtt cgcgtaaatc
     5761 cttctcgatc ttgatccggt cgatatcggc cgaggcataa tcgcccaaaa cccatttctc
     5821 gccgccgaag aatttgtcca ccttggagag tgcgtcctgc atgaacttcc acccgcccct
     5881 ggtgaacgcg cccggaacgt ccttgttatt caccagcacc tgggcggagc cggggaattg
     5941 ctcgttgaaa ttcaccttgg ggttgttcct ggcggactcc gtcaacatga actgatagat
     6001 gctctccgtg cctccaaact gggccaggta gcgtcgtgaa cggtcaacgg ctccggcatc
     6061 ggcctcggtc gaatacggat tctgcgcggc cagttcactc gcgtagaaat cgaactgcct
     6121 gcgcgccagg tccacccgct ccggatcgag ctcgcggccg gcaagccacc ggttcatcag
     6181 caccggtgac agaaaagacg gcaccgttcg cttgtgttcg taggtggtaa gaagatacgc
     6241 cttcaacgtg tcataggcat agccgaagtc gtcattcgca ccgggcgaag ccgggcgctg
     6301 cgccagccac tgcgccatgc cggcctgtgt atggccgaac aacaatcgct tgaagcggtc
     6361 gtaatagatg cgccgcgcgt gcggatacat gtcgtcgccc gcgtagagcc cccaccgcag
     6421 gcggagcggc ggtccttcgc gctcatacat ggcgaggcgc gcgtacgcct gccgcaaggt
     6481 ttcgagctcc cgcagagatt caacggaagg cagcgtgtcc gatgccgcgg ccggtgttcc
     6541 gaggttgcgc gccgcctcga taacctcatt ttcaagagcg cgatttccaa tgaacgagac
     6601 ggtgaacgcg atacctaaca gcaggaatgc gaccgatgcg gcagccagaa ggaaccggcg
     6661 cgtgttgctg gatttcacgc ttgccgagct tgctcccatc gccgcgcggt cctgaaggat
     6721 cacgtcattg aacagatggg tgagaaaaag ccattgcgga acgcgccggg ttccggaaac
     6781 gggctgggcg gcaggttgcg gcggggcttg ctgggctcca acccggaatg cctgtgtcgc
     6841 agtcgcgggc gcctgcatct gccgctcggg ctgagatgga atggcgggaa ccggtgcgct
     6901 ctcgctcacc aacaccggac ggacgccgga gaagtagaat ccgcgtaaaa acggcgaagc
     6961 acggagctgg ctgggacggc acaggtccac caggaactgc accgccaggg ttctgatttt
     7021 ccggaactcg cgcggaaatt cataggttcc ggggagcttg gcaggatcgt tctctcgtgc
     7081 caggaaattg atgcgtttgt cgcaaagcga gtgaaagagg cgctcgaaag ccgcggtgag
     7141 cctgcgcgtc tcctcctccg catacacacc ttccgattgc ggcggccgga tggttagcgt
     7201 cgtcccggct acctgtcccg actcttcgtt ggcgagattg cgaacgtagt cgaggaagaa
     7261 cggaagacgg tcggcgcgcg tgaacagcac gtacaccgga aagctgatcc cgagggtctg
     7321 cgagatatcg ccgagcctgg catgaaggtt gcgcgccatg gacgctacag cctccgaggc
     7381 gcccggtttg gtgaatattt ccgagtccag gcagacgacg acggcccgcg gtgcctgtcc
     7441 tccggacttg aatgcagccg cgagcttgcc gggttgaagt ctctttatga attggagcca
     7501 gcgtgaggtt tccccaagca gccgcccgcc ggcttcgacg aacaccgtgc gtccggaaag
     7561 ccagaaattc gcggtgcggg tcggagtgac cgcgttgtcc tgatacacgt ggccggccag
     7621 cagctccggc tccaggccgc agtgcagaac ggtgctggtc ttggcgcttc cgggaccgcc
     7681 gatcacgacg atagcgggca cgttgcggat gtcgctttcc tgcacgatgc gcgccgaccg
     7741 gaggcgattt cccgcctcgc gaaacagcag gtccagttcg tcgcttccgc ccgaagccgc
     7801 tgtggagtcc ggaacggaat cctgggcggg ggaagtcttc ctgaacttgc cggtgaacca
     7861 gagccatgcg cccgccgcca aggctcccag aacggacaac accagaccga acaggatgta
     7921 agatgccccg gtcagcccca gcagcctgcc aactgtccag acgagaacca ggtaccctgc
     7981 ataggcggcg gctgttgcaa gttgagaaga catttgtcag gtccccgttt ccgagccgcg
     8041 accgcgaggg agcggtttcg cgcagtcgaa gcggaagcat gtgataccgc ttgctaacgc
     8101 gcgcggctcc atttccgagc cgtgaccggc gccgatgccg cggatggtgc cgactcccga
     8161 acgcagaagg agcatgtaga caatgaacag cgcaaccgcg agtgagacgc agcagatcgc
     8221 cgcaatgaaa gcccgtttca accagggatc gccgcgcgtg acctgtatgc tgtcgccggg
     8281 cagggcccat ccgggggaca gttgggaagg agtctggcgg atgcgttgta ttttggcagc
     8341 caccattcct gtaatgcctt gcagttcccc tcggccccca atgctatacc ggcccgcgaa
     8401 gcccagcagc atgcatagat ggtaaacctc cagcacatcg gctacatccc gcgattcatc
     8461 gcggacaagc agcctttgaa gattccggaa aaagacttct cctgcgatgt gatgcccgta
     8521 aaactcttcc tgtaagggcc gccggggcca atcggtgaag acagggctgc ggaggttcag
     8581 aatggactca tcgagaaacg ccacgacggc aaatttcgcg tcctccacat cctcggcgga
     8641 gtaaccggcc ctgcgcgcct cctggtccgc catccggatg gcctctcgaa tctggccgcg
     8701 gaaagccgcc gcgtcgctca ccgtttgccg gttcgaccgc aggcgtacaa tcgccgtcag
     8761 gacctcctga aaggccagag cgagattctc cgcgcggcgg gcgttctctg tcatccaagt
     8821 ctcctatacc ggcttgctga cgcgcgcggc tccgtttccg agccgtgaac gcaagagagt
     8881 ggcatattct caggactcca ataccacaag cagctccaat tgcgtatcgg gcaagtctcc
     8941 cggcacatag atgccaatct cgcgcgtctt gacgatgctg tcccaacaag ggcctacttt
     9001 gtcgacgctg aaatactgca tatccacctg ggcggagaca gtggaaggcg gaacgggcag
     9061 atgcgtaagc gcaagcccgg ggagtgcgcg tttcaccagt tccgcaacat acttctcgga
     9121 acagaccttc acgatttgcg gcgtcttcgt aatgaggtca acttcgcctg cgggcgaccc
     9181 gattgccagc acccagcggg agcggtcgag gacacgctga tctgtaatct gacccgcgta
     9241 aaagtaatcg gctgtttttt ccagagaaat tcgcaggcag ttggtgggaa cgatggcctc
     9301 caggtgtttg cggatgtgcc ggtccagttc ctggaagcag cgacccagtt cgttgtggtc
     9361 gtaaagcgga atcgcacgag ggtgggaatc gatggcgaac gtgcaaagcg cgccgcccag
     9421 ccgcgccatt tccacgtata actcctcggg atgtccgcgc ttggtcatta actggtggcg
     9481 gaggggagcg atggcggagt tgacggcatg cagcatccag aagctcgcga tatcgcgtgt
     9541 cgagtactcc gcccacgcgc ccgcgccgga tttcctgccg cgcgacaagg ccgtgctctt
     9601 gtcttccaga atttcgacca gcctctgcaa catcagcatc agcggttcgc tcgcgccgat
     9661 ccgcacgcag gcggggatga actccggatc gtaaatgaaa tgaccggcac cgtcgcgcag
     9721 cacgcgtgca agcggcatcg tcagcatgcc ttcggtatct tcggtatcca gcagaaaccg
     9781 gatgttctta cgccccaact gcaccggctt ttcctcgcgg ccggtgttgt cgtcgtgaac
     9841 cacctggttt tccgctacat agcgtgcttc catggcaaac tcggagtcgc cggcggcaca
     9901 attcattcca tcctgtttac gcgtaggaac ggcgagcaga accgtcaaaa aatcccgggt
     9961 gggaggaaac aagtccgcaa tggcgcgaac ggcgggcagc gggtccgatt cgggcatctg
    10021 gaagagcagg ccatcgggaa gaataccgcg tgcgtgtatc aacccaacgg tgccgttacg
    10081 caacgcttcc gcatccagtt gatagccggt aaacccatgc ggctcgaacc acacgctcga
    10141 ggtggcaaag cggattgaat cttcgaagta ccggttctga acctggaaat gatgtgggcc
    10201 taaatacatg ccttcggacc acacaacctt cgaaagaaat ttcataactc cccgcagacg
    10261 ttcgtcagcc cggaacgcat tcggagcagg ccgccgtgaa gatgtatgga tggggtctga
    10321 ctggcatcag tttcggaggg atttcgacgc aatcggacat tccccttccg ctcgtgcgcg
    10381 cgccatggat aatctgcaac agtctactct atcatagggc aaaagagacg gaattttgat
    10441 actcttcagg cagataaccg ggaggaacgc ggcgtgagtt tctacaagaa gtatgaatta
    10501 ttggaactgg tccgcgacga cggaataaag acatttgctg caagagaaat ccggacgggg
    10561 atgccggttc aggttcattt gttcacggga gtttcgcccg ctgagagcga cctgctgtgg
    10621 agccagttgg atgcgattcc tctggaacgc cgccggggtc tctttgccta tgccgagtac
    10681 gaagggacga ggtacgttgt caccgagcca ctgacctcgc cggagaacct ccggacgtgg
    10741 gtgggcgcgc gtaccactcc tgccccagcc cccccaccag cgactgaaga cgtgctggtg
    10801 aaggcgggct cgtggaagat tccctcggcg ctgttggaga agaagccgcc ggagccggag
    10861 ggccaacctg ctcctaccga gcgaatgaac attgaggagc ctcccgcggc tgcacctcct
    10921 ccggagcctg aaccaggtga gttcactcgg atgttccggt cgccgccgga gccggaacgc
    10981 cccgcggcgc ccacggagcg aatgaatgtt acggagcccc cagcggctgc gccgccaccg
    11041 gagcctgaac cgggtgagtt cacacggatg ttccagtcgc caccggagcc accagtttcc
    11101 caaccggcga aaccggcacc accgccggag cccggggaat tcactcgcat gtttgcaccg
    11161 caaccgcttc ccaagccgga gccgcagaag ccgccccctg cccaggtcca gatccgaaag
    11221 ctatcgcccg aagagcaggg acttgcgccc gaaggcaaac cgcggcctgt tttcctgacg
    11281 ccggagcgcc ccgcgagcgc gaaaccggaa cccgctcctc cggcgcagtc cgaaccggat
    11341 gaattcacac aaatgttcca ggcgccggaa tccccgcaag aggaacctgg ggactttacc
    11401 aggatgttca gcgcgccgcc cgcggagccg gaaccgcaac cgtcgccgga ggagtccggg
    11461 ccaggggagt tcacgcggat gtttcagagc cctcctcaac ctgaaaaacc ccgccagccc
    11521 ccgccgcctc ccggcgagcc gggcgagttc acccggttct ttgaaaacgc tccaagacag
    11581 gcgccatggc cgtcttccgc atcacagggg acgcctcctc ccccatccca tccaggtccg
    11641 ctggatcagc caggggagtt cacccgtatg ttcggcggag acgttacgct caagcagccc
    11701 gctcctcgcg agcctgcccc gccgtatgga ggcgcgacgc aatcgttttc gccgccttcg
    11761 ggaccacacc ctccatctgt tccttctacc gggtcgcgcg gcgctccggg cgagtacacg
    11821 cgcatgatcg agaggcctcg ggaattctcc gccgaaccgc aaccgccgca gcccttcaac
    11881 ctgggcgagc agcgtgccgg agccgcgcag aagccctctt acctgccgtt gatcctgatt
    11941 ttcggagtgt tgttccttct cgctgtcgcg gtggtgctct ttttcgtgct gcgcaagcct
    12001 tgaggcatca ggctgcctgc ggcagcagaa tgatgagttc gagttggggc tcgggaaaat
    12061 ctccgggaac ataggcagcc aggttgcggg ctcgcgtgat ggcttcccac gccggtccgg
    12121 tctgactcaa agagaaatac tggtggttca gcttcaccgg aatcgcgctc ggcggccgcg
    12181 gcgtgtgcac catttgaacg cccggtaatg cctggcgcac gaggtgttcg atgtggttcg
    12241 ccgaacagac tttgatcagg tggggcgcgc gtccgatgag ttcgccttcg ttgatgggcg
    12301 cgctgacagc caaatacatg cgtgtgttgg aaagatagcg gtcctcggcc agagaagtgg
    12361 catagatgtg cggctgcacc agcttcagcg gcagagagac gaaattgctc ggcacgacgg
    12421 tatcgagcag aaaccggagt ttctcatcga ggctcgtaaa acaaagcgag aggtcgtcat
    12481 ggtcgtatgc cggcaggtcg cgcggatgca cggacgtcga aaatgtcgtg agggagccgg
    12541 ccagcgacaa catcgcggaa tagagcgttt cgggatggcc gcggcgcacc tcgaacagat
    12601 gctgcaacag cggaaaatgc gaattgatgg tatagagtaa ccagaaattg gcgatgtcgg
    12661 ccgtgccgaa atcggcaagc gtcaggtttt tctgccggcg cgtcccggcg agcatcgtgc
    12721 tttttgccga caggatctcc accagcctgc gaagaatgga gaccagatat tcgctcgccg
    12781 ttatatccaa cagcggcggc acgaagcgcg gatccagttg aagagttccc gcgccggtct
    12841 tgcgcacacg gccggcatgg agcgcggagc aaccctgccg cgactcgcct tccacgagaa
    12901 ttctgaagtt cttgcgcgct acctggacgg gccgttcgtt cattccggtg ttctcgtccc
    12961 gcagcagggc aacttcggcc aggaatcgcg tatcggcgcc gtgattggcg agggcgatgt
    13021 tgagtccgcg ttcacgatag tcgggaatgg caagatatac atccagttcg ttgcggtcct
    13081 gctcgaaagc ctccgcgatg ggcttgacgg gcggggccgc gtcggaatcc ggaatatcga
    13141 acaggagccc gtcgggcaga atgccggaag acgcggacac ggcgaacatt cccgctgcca
    13201 gcgcctcctg atcgatgcga agcgttcgaa atccccacgg gcggaacgcc agggcgtgca
    13261 actggaactc cagcgaactt tcgataaagc gatcctgtgc ctgaaggtat tgcggcgtca
    13321 gaaacgtacc cttcatccag ataacaggct gtagggattt catcggtttt caaggtaaca
    13381 gaaccaggag atgaaggcaa aatacaaccg gcacgtgacg ggtttttgtg gcccgctgcg
    13441 cgttacgggt tgtaaggccg agaaaaacaa aaaaagatga tccggtgggg tgatggtgat
    13501 ggcggtgctg ggctttccat ccttgactcc gaaacctgcg gaagatatac tcaacccact
    13561 agataatccg gattgttttc cggcggggaa cctggtgcgt gccgctggat ttcgatgtaa
    13621 ccgggaggcg cggaaaactt accgcgcgag ggttgcgccg cgattaaatt gcgggcgacc
    13681 ggataaagaa atggatacct cagcaggaga aaagagggac ctcaattaaa tggcaagaga
    13741 aagcacacaa cacaagttgg atcgtgtccg gcctccgcgc gtccacgtga cctacgacgt
    13801 cgagataggc gacgcgatcg agatgaagga attgccgttc gtgatgggag tgctcggcga
    13861 tttcacggga caggccgaaa cacccttacc caggctgcgt gaccgcaaat tcatcgaggt
    13921 gacaccggat aatttcgaca gcgtgctcga aggcatgaag cctcacctcg cattttccgt
    13981 ggagaacaag ctgagcgagg atcctgacgc gggcaatatc aaagtcgatt tacgcttcaa
    14041 gagtcttgac gatttcgagc cggagaacgt agcacgccag gtgaagcctt tgcgtgagtt
    14101 gctcgacctc agaacgaagc tgacggatct gcggggaagc ctccagggta acgacaagct
    14161 cgaggaactg ttgctcgaag ccgtaagcaa tacggagaag ctggacaagc tgaagggcga
    14221 gatgacaccc ggagagccga aggagaccgg caatgagtga gccgcagaaa tcgatgcagc
    14281 agggcgcggt agagcagact gccgaagtca gcctgctcga tcagattgtc gaacaaggca
    14341 ggctggcgcg cgacgaatcg gctcgggaac gcggaaaaga catggtgaag gaattcgtcc
    14401 agcaggtgct ggaggggtcc atgaccgtgt cgaaggatgc ggagacgatg atcaacgccc
    14461 gcatcgcgca gatagaccat ctgctttcga ttcagctcaa tgagatcctt catcacccat
    14521 ccttccagaa actggagggg acctggcgcg gtctcaagta cctgctggat caaagtgaaa
    14581 ccggcaccca cctgaagatc aaagttctga acgcatcgaa gaaggaactt ctccgggact
    14641 tgcagcgcgc ggcggaattc gatcaaagcg ctctcttcaa gaaggtgtac gaagaagagt
    14701 tcggcgtgtt tggcggagcg ccgttcgggg cgatgatcgg tgattacgaa ttcggaaagg
    14761 gccccgaaga catcgagttg ctcgagcgga tttcgcaggt ggcgggtgcc gcccatgcgc
    14821 catttatggc agcggcccag cccgagttct tcaatctgga caagttcacc agcctcgacg
    14881 cgccccggga tctggcaaaa gtgttcgata ccactgagta tgcgaagtgg aaatcgttcc
    14941 gccagtccga ggattcgcgc tacgttgcgc tgtgcgtgcc ccatatcctg atgcggcttc
    15001 cgtacggcaa ggacacgaag cccgtggacg ccttctccta cgaagaagcg gtggacggca
    15061 ccgaccattc gaagtatctc tggggcaatg ccgcattcgc gcttggcgcg cgtattacga
    15121 atgcctttgc gctgtacggc tggtgtgccg ccattcgtgg cgtggagggt ggcggactgg
    15181 tggaaggtct gccggtgcat aacttctaca ccgacgaggg cgacatcgcc atgaagtgtc
    15241 cgacggaagt tcctattacg gaccgccgcg agaaggaact ggccgacttg ggattcgcgc
    15301 cgctggtgca ctgtaaggga accgactacg cggcattctt cagtgttcaa tccgcccaga
    15361 agccgaaact ctacgacctg gaatcggcga acgccaacgc caggctttcg gctcaactgc
    15421 cctatatcct ggcggtctcg cgcttcgccc attatctgaa ggttatgatg cgcgacaaga
    15481 ttggcagctt catgtcgcgc ggcgactgcg aacgctggct gaatcagtgg atccagaatt
    15541 acgtcacacc ggacgatcag gcgtcgcctt cggtgaaggc ggaaaggccg ctgagggaag
    15601 caagtatcca ggtggccgaa gtggccggaa agcccggcgt ctacaaggct gtggcatttc
    15661 tgcggcctca
//
LOCUS       DASTMK010000108        16572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_389659, whole genome shotgun sequence.
ACCESSION   DASTMK010000108 DASTMK010000000
VERSION     DASTMK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16572
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389659"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            788..1141
                     /locus_tag="VFQ79_04350"
     CDS             788..1141
                     /locus_tag="VFQ79_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138913.1"
                     /translation="MTGRALGLHQDKKPLPALTSTTWELDQHRQAVLLALYGEVCDGW
                     RTLTDVRFKLLGLLPIVSVAVLITLLSKSHGADALAPTERTGIALLGLLFTGAIRLYD
                     LRNTDLYNDLVGRGR"
     gene            1312..5217
                     /locus_tag="VFQ79_04355"
     CDS             1312..5217
                     /locus_tag="VFQ79_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138914.1"
                     /translation="MGLQTSELVMVALGVRGDAPPVALQPPLVDGIHLRWAFRRENGF
                     PWYGYYLFRRPHEFRGERCLAADLLGRAPGTDLGTTFAASLGTLSSDASLVLSDDFPA
                     AGRPEVSLDGHSAVRLDLPADEPAFRVRVQVGFRQSGEGFKCIDFRLLAAGLVANPVS
                     VGEAIFEAQNERGRPLAQIRLAPSDTARSGVALDASGRVEIKLPAATRAVRLDMTGNA
                     RAPEFEALDEMGRVIRTASPALDSQRPRTVELVAGSDFQQFRIRSSQGEVLVHRLCWT
                     VPAARRIELPVTAYDGLMVVDTGTVSGAPGQVTEVTLNFDRITAIEIGGGPAALVDLC
                     AQIVPDVAEKDWQPAPNFPQPMLLPLTHPDYPLRASAQNVPAARTVALNRVVYGPAAP
                     WSGANFSSLHTSLAALVRGGPSRPMAAPERAAQNVQGNPTPAAPGIDPPSIPSLHPLD
                     LVLLGSLHPAVAQMVGLYWADTTADPRSSYDYLLVADRGNVANGNVATLADHILRHGW
                     NDVDAWITFDRRMAPAPPLTPPDSVRAYALPGGTYRERGGSNAGVQEADGNVGLTWPQ
                     PLNGAGYLRPGQPVFYHVWRDNQGDGVNPQPSGQTATLVTTGGPLLISHQLAAPAETP
                     QYPGDWPPFSLPALDLDLDEGWYGYQVNSIDIFGRFSPKSAFAEWWQWAPVPAPKPWY
                     YIDPPGARQVHASSVRIIERNPPPPPAFIEAWALDPADPVLVRDAALDAWRQSLPAGQ
                     RNTLVGLRVRWRWNVAQQRQAPDTREFRIYWHPGTDPPVGWESVASWQIRCDVNAYAS
                     NVTVASNGDRSYDVLLPATAGQGPFGTGVPLNPTRANPIAYANVTVTAADDAAQADTW
                     PGTGALAGRTGNESRAASVSKVYRVLREPPPPPEPVVDSDRLYSTPADWHAQSYYTFR
                     WRPGADVFTHVFRALDESVFAADWALQPRPDLQPGDAARFPDPVAEPTWNLAKRRTVC
                     TALNALNAFPKSDAGKQQALLAYRALSHDALRVLANMPGCEKAFVQLTVKALDPADAA
                     TADRRGPDDALGYVVRPGLRAYTDKLDGRATNRYLYRAVYVDAAQNRSAMGAVGTPVR
                     LPNVVPPRAPVLTKVTGGDRRITLSWASNREPDLLEYRVFRAETKDDAGDLRLMQQVG
                     TVAADPDPALRPAAEQWDDSPVPGLKDFWYRIVAVDRSDPVDPRGGGGNVSQPSSIVR
                     GRAQQPPPAPPIVQTAVWNTGHTAVQVSWQISDPALVPRLERRQNEGEPWLVAAGWLA
                     PGTLDATDTPPDPALPYQYRVRVRDHAGQQAESQPVATS"
     gene            5237..7255
                     /locus_tag="VFQ79_04360"
     CDS             5237..7255
                     /locus_tag="VFQ79_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138915.1"
                     /translation="MPNALRFLFSGNDLQKVVLNSIQVEDIVAGAPAGSPPIASMRLN
                     FRQSDPGPLQIRPLFPGTMRFLVDPAAPGAIPDPATGSFTEADYPGWKTLGRLLIILS
                     DQTAGEIAGMAPMLPVKPNRIWYGPVRLPPGFLFETLTAGLKKAPIPASGGRTIRTSN
                     ADWRKFAIAEFLAGRYTPLLVTSANAAQDDRVRFVMPTVEVPNSGDIQLDVTTALAQK
                     PQDALDSEFENPAPPVGQEEPTHPRNGLIPAREVYRRLRPGFVAEPAATALMNAILFD
                     WPEGPLFWPIQFTRTSEATNNCSVHFPRQTVHMLAGPGDSQDQTLPAHGFVYFRQAPP
                     PTGQPAPPAPILQVSLTGNMKWLPGGGTSWRSPGQTAGVAIDFGAIGTPHVSLRLPMS
                     EAMFSDTSRPAPRGARCAYLSMRRTVRALIDNRIAGGRLNFGVNTTSAVTRGIIRRAF
                     QGTPASVNAVAANAPNPAGDPNVLALTLEPMWRAFFPDPAPQQPIPGAPANPTVLDEG
                     EMVYRLWQSIAIAFQSNQTSRNFSDAHIGRGAPGAMVAANLAAFHVDPVRNVGDSDNA
                     YFDRVAGSMLAGLQPGALLQFWNLNSDFEDLKNRSVPGGANPQIASYGHSPVFVDYVL
                     DVTGNVIGITILDQFGESTSAVAGPAGNRRIQWDGDDQQIWIAANWSE"
     gene            7259..14806
                     /locus_tag="VFQ79_04365"
     CDS             7259..14806
                     /locus_tag="VFQ79_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138916.1"
                     /translation="MPYEITGRALYPLSEILQRQEFPLAELIPQDALDTVFGGLYYTD
                     AEAYSTSEGLHLNLRLAFEGELALSPPGLPGIALVAASGGMGWTSLRAEFVIGPEPSA
                     TLTDVPLVLRLDRSLLKPMRSRTEVDDSKPGVDLALGTVALKFSPDGLEGDLDISVSL
                     PLSMIGDTGVLIEATNVRPILGPMNSSLPGVDASFRGVFMESVRIYLPEGLPDLAPSD
                     LQITNCFIGTGGFTGALALNYNPVYDAATKTFSGPGSGSLFGMALGLTRLSIRLTQNT
                     FEEAVIAGSIVLPFFDELLDIEIGLALDGGFMVAIASPGGLVTLTKPDLLELSLESIR
                     FSLEDGVFTAGVSGSVHPLVGGIEWPTFRVEELSIDSQGHVRIQGGWLDLREQYVLSL
                     GGFQFEISRIGFGSTEEGRRWIGFSGGLKLVDGITAGASVEGLRVAWDPNTGDISVTL
                     NGVDVEFLVPGAVYFKGYVAMTEPAPGVTRFDGQITLDIISVGLLIEAQLVVGYDRVN
                     DYPFFAIYLGVELPAGIPLGQTGLGLFGFAGLFALNLEPNRRPDPAQPELPWYGIQPG
                     PSWYHAPPRVGVAELRKWVNRQGSLALGAGVTIGTVSDNGYLFAGKFLLGIIFPGPIL
                     FIEGRANILKERASLRDEPVFRALVVLDARAGTFLMGLDARYAYDDTGALIEIGGCEA
                     FFDFHDVSAWHIYMGIDEPRERRIRAWIFERLFEANAYFMLNAERLRTGAWIGYEAHW
                     DFGPLAVELEAWIEGNAELSFKPVYFKGSLWLHGKIAASVFGFGFGLGADARISAEVF
                     DPLHILAELSVSVNLPWPLPDFDASIKLEWGPEPDPPLLPVPLKEVSVEHLKVTTTWP
                     LPVTGGTILTLPDPDGDGDGFFDGTAPAPPSDTQLGPSDLSPVVPLDARPRITFGRSV
                     HDLATIGENPSPVFPDSQPEAGWEWIGDPALNQGPARVRPALTELALECWNGTRWEPI
                     ARKATTSNPVGVRALYGSWAPVPQLPAGNVQPGSPPPTANVKLWLWSKSPYDFSRHTG
                     GQWDEWFDHAYPDYPCLPLPPDREDCCDFSGLSAGDIPAPPWGCSAHPEIELTWAAPP
                     RPVVRVNAGQKALCFPRGSHVTVQIGARTKRVRVSVQYEAGKETRACANFTDEAVGDG
                     ENPRRTGAFRFVVYDAAGQLLPRTRVIQVATSTGGRRGLDAGFRATIDLPASDFVELD
                     LTRLNQPARLQAIAAGGGLIAETVMSAAIGASQVVRLERVRGQREIVRVTVTSPADRV
                     AIHSVCYGVRPRSIVGRAIDRSGRELGAYRDVNGLIDVPGRDVVAVRLDADGGAFCVT
                     RICVTVGLDQADRIQREEMLRHMMDELVRWEADDEVLAPWTRYRLKIVTTLDVRGFSY
                     DAAFNSTRTITQCAYFRTEGPPGLTTYSVPIGHPQENAAAPAGASDPPQFDSGLQDLS
                     RYVAQTVPPTVPAAGEKPPLPKPVYRAYDVGVRFNENYVETMYRLAGRDLTLAIYDNN
                     NEPVRDHRGRLLISPNRWGRADTVDLAASEERWISHINETACLQLDRTRIARDVTLGA
                     DTHVLDPQTVYEARLLPLLMHDAFADYTAGAQARGAGATLVPTTGAGWTVRDEGTDQG
                     PSQWIVREAGNPPSRYVEQTTNVSAGAARRSSVFAGGTLLICAADPRAGVPQADQPAN
                     WTDYRLSVYIRSLDDDIVGAGVRWSGASGYLLTLDRELNRRRLLRLVNGAATVLDESA
                     GGYDTNRDYLASIEAAGPRIRVFLDGAPLFDVSDNRFASGSVALYCGQNAGGHFRDAR
                     VDDLRGTAPVVYRLKFTTSDFTDFRHHIHSFPSQVFRAAFPDLNDVAASIGAALPLTG
                     ATQAPQEAEARAYDTLATKALGTAARQPVALLDVTRVEQGGAALGFLVRTADPIDWRR
                     TALSLLRAPQQPLMPDAPSIPRLIRATFATGATPQPNDESITVLLDTGMDLAGWQIQR
                     RTLPSANAQALAAETVLFRANLDTDQGPDSIQSDSLWKPRLNNLNDFDMADSPGGIGT
                     SMWAAGAGFLRQDGNFRVTDPPLTEPPPRLGTYAVRALAGEWRDVRFSVRLRANTGAA
                     AGVVFRYQDVQNHYRFAFDSARNVQELTKRVNGRTSLLFRKPFNFAAGRFHALVVEAL
                     GARLRVTVDRLQIAEVFDDDLAQGAAGFYTFLQPLPRFDQVTAASLTRTLGPWTIYDH
                     GDVRATSEWRVENRLLRQSVDLFTAPPAAATAQRSGSAAVTGDAAWTDVRITAAAQPE
                     AAGIAGIVFRWRDSDNHYRLVFDGVNGQRWLVRRMGGVSTRLWSGAGQLGTMEVAVEA
                     IGNRLRAWVDGAVLFDVYDDALAAGRAGVLSMDDSAGVWSAFEVRHVQPAWEDWHVFG
                     TNEGWRAAGRRFEIRAGRSADSAAPASGAGEERLFQELASAGFQPRLRAPGVDLRLVG
                     NDGEPGHMRRFLPDGAYVAQATARILRSADGTGMFVFVPGAGPAGAGLPSGEYRLEFT
                     YRRDNTNADPASLVLSQASDTADETAWLDIPWPAH"
     gene            complement(14883..15338)
                     /locus_tag="VFQ79_04370"
     CDS             complement(14883..15338)
                     /locus_tag="VFQ79_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein E"
                     /protein_id="HEU0138917.1"
                     /translation="MLITVSKLEGDQLQVNICPRQLKDGENAALTIPLCVTGTAAELD
                     AELVCQVSNFVASHVGLNTNLAAIEKEIAEAEKAAREEARKKHKVVGNGGKKSTDSLP
                     ATPPAPAKPEPEQPQMLSLFDQQSQSAPTKVPILWSRAAPAAGYTPVRR"
     gene            complement(15549..15941)
                     /locus_tag="VFQ79_04375"
     CDS             complement(15549..15941)
                     /locus_tag="VFQ79_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138918.1"
                     /translation="MIDIDWSVLHDGLVMANGSSRNSWGGFYSGTVAREIGRFAARKG
                     QHYSVVLDIRRDGGELSATDPKLLVQTDPGAWKDAVVGFALASLLRTIGIAIFVIAGL
                     LTLVLTPLFGRLYRLHQRRKKRPHRGLN"
     gene            complement(15975..16232)
                     /locus_tag="VFQ79_04380"
     CDS             complement(15975..16232)
                     /locus_tag="VFQ79_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138919.1"
                     /translation="MRYRPKRHQYVALVLLALAGVVYCIHPAPVDIWRWKPIAVPIQL
                     KVGRVHTDVFEAGLDTNYRLLVESERRIEFKRLECMLGMVD"
     gene            complement(16336..>16572)
                     /locus_tag="VFQ79_04385"
     CDS             complement(16336..>16572)
                     /locus_tag="VFQ79_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138920.1"
                     /translation="RRPYTAKQGQYLAFIFYYTKIHGFAPAESDMQKYFKVSPPSVHQ
                     MVVTLEANGLIARVPGQGRSIRLLIPREELPDLE"
BASE COUNT         2859 a         5282 c         5291 g         3140 t
ORIGIN      
        1 gctgccttct ggaagcaagc ttctctattc gattgattcc gctatattat gcgagcgtca
       61 gtaagtctgc ttgctccctc tcttcctgct tctcccggcg cgcgcgggta gacttccttt
      121 tcgtcggtgt ggccatggta actataaata ccccacagaa ggtgataagg ttcgctctga
      181 ccgtaggaag taagtgggga gagtaggtgt atctccccac agaaatgagt catgctgttc
      241 tggcggccgg ctgatcgact aagatacccc gcagcaacgt cctcgcgtcg tgtaggcggc
      301 gcttgaccgt tcccaagggg atacccatca taagtgcgat atctgccagg ctatgaccat
      361 cgagatagtg agcggagacg gtatcccgga gtgctggaga tagtcgtgtg atcgcagagt
      421 ggagtgcggc taggcgtgta tcctcgacat caggcggggc agtcaatctc tggtccggac
      481 cgaggaacgt gtcgtgatgc gacgtctgct cgccgtcctc gcttgtgatg ggctcatcga
      541 ggctgaatgc ctcatatttg tgtcagtggc gccgagcgaa ggggccaaaa tggcagggcc
      601 agaaggggcc acggcgccaa ggcttgatgg cgctgtaggt gtccccgggt gagggatggc
      661 aatgtgccca acaaggcgat tcgggtatcc gtcgagcagc tcagacgcgc tctgataccg
      721 ctgatccggg tcttttgaac ggcccttctc gacgatctga cgcgccgccg ccggcaacac
      781 gttccgtatg actgggcgtg cgcttggcct gcaccaggat aagaagccgc ttccggccct
      841 cacttctacg acgtgggagc tcgaccaaca tcgacaggct gtacttctgg cgctctacgg
      901 agaggtctgc gacgggtggc gcacgctgac cgacgtgcgg ttcaagttgc taggcctcct
      961 gccaattgtg tcggtcgcgg ttcttatcac cctgctttcc aagagccacg gtgcagatgc
     1021 actcgcaccc accgagagaa ctggaatcgc gctgctcgga ttgctgttca caggcgctat
     1081 ccggctttac gacctgcgga atactgacct ttataacgac ctcgtgggtc gtggccgcta
     1141 aattgaggcg gagctcggca tacataccgg ccactttcgc ggccgaccga attctcgcgg
     1201 gtgggtgcag catgacaagg ctatcggtgc ggtctatggc ctgtcagcgt tcgcatgggc
     1261 tgccgccgct gttcagccat ggctgccagc gttgttccgg taagaaggta catgggacta
     1321 cagacatcag aactggtcat ggtcgccctc ggagtgcggg gcgatgcacc cccagtagcg
     1381 ctgcaacccc ctctcgtaga cggcattcat ttgcgctggg cctttcgccg ggaaaacggg
     1441 tttccctggt acggttatta cctgtttcgg cgcccgcacg aattccgcgg tgagcgctgc
     1501 cttgcggcag acctattggg gcgtgctccc ggaacggact tgggcacgac cttcgccgcc
     1561 tcgctcggta cgctctcgag cgatgcatcg ttggtcttgt cggatgattt tcccgctgca
     1621 ggacggcccg aagtaagcct ggacggccat tcagcggtgc ggctcgacct accggcggac
     1681 gaaccggcat tccgcgtccg cgtccaggtg ggattccgac agagcggcga aggcttcaag
     1741 tgcatcgact tccgcctgct agcggcgggc ttggtcgcga atcctgtcag cgtgggcgaa
     1801 gctatctttg aagcgcagaa tgaacgcggc cggccgcttg cacaaatccg gctcgcacct
     1861 tccgatacgg cccgcagtgg cgtggcgctc gatgcgtcgg gccgcgtcga gatcaagctg
     1921 ccggcggcga cgagggcagt gcggctcgat atgactggga acgcccgggc gcccgagttc
     1981 gaggctctgg acgagatggg cagggtgatc cgcacggcgt cccccgcgct ggactcgcaa
     2041 cggccgcgga ccgtcgaact tgtcgctggc agcgactttc agcaatttcg catccgctcg
     2101 tcccagggcg aggtgctggt ccaccgactg tgctggacgg tgcccgccgc gcgccggatc
     2161 gagctgccgg tcacagcgta cgacgggctg atggtggtgg ataccggcac agtgtccggc
     2221 gcgccgggac aggttaccga ggtcacgctg aatttcgacc ggatcaccgc catcgagatc
     2281 ggcggtggcc cggcggcgct ggtggattta tgcgcgcaga ttgtgcctga tgtcgcagag
     2341 aaggactggc agcccgctcc caattttccg cagccgatgc tgttgccgct aacccatccg
     2401 gattacccgc tgcgggcgag cgcgcagaac gtgccggccg cccggacggt tgcgctgaat
     2461 cgcgtagtct atggtccggc tgccccctgg agcggcgcca atttctcttc gctgcatact
     2521 tccttggcag cgctggtccg cggggggcct tcgcgcccga tggcggcgcc ggagcgcgca
     2581 gcgcaaaacg tgcagggaaa tccaaccccg gccgcgccag gcattgatcc accgagcatt
     2641 ccttctttgc atccgctgga cttggtattg ctgggatcgc tgcatccggc agtggcccag
     2701 atggtgggcc tttactgggc cgacacaacg gcggacccgc ggtcttctta tgactacctc
     2761 ctggtcgcgg accgcggaaa cgttgccaac ggcaatgtag cgacactcgc agatcacatt
     2821 cttcgccatg gctggaacga cgttgacgcg tggatcacgt tcgaccggcg gatggcgccc
     2881 gcgccgccgc tgactccgcc ggatagcgtg cgggcgtacg cgctgccggg cggcacgtac
     2941 cgggagaggg gcggctccaa cgcgggtgtc caggaggcgg acgggaatgt gggcctgacg
     3001 tggccgcaac cgctgaacgg cgccggctat ctgcgcccgg ggcagcccgt gttttatcac
     3061 gtatggcgcg acaatcaggg cgacggagtc aatccccagc cgtcgggtca aacagctacg
     3121 ctggtcacca cgggcgggcc gctgttgatt tcccaccaac tggcggcacc ggcggagact
     3181 ccgcaatacc ccggtgactg gccgccgttc tctcttcccg cgctggattt ggacctggac
     3241 gaaggctggt acggctacca ggtcaactcc atcgatattt tcggccgctt cagtccgaaa
     3301 agcgcattcg cggagtggtg gcaatgggct ccggtgccgg cgcccaaacc gtggtattac
     3361 attgacccgc ccggggcccg gcaggtgcat gcctcctccg ttcggattat cgagcgcaat
     3421 ccaccgccgc cgcccgcgtt tatcgaggct tgggcgctcg atcccgccga tccagtgttg
     3481 gtgcgcgacg cagcgcttga tgcgtggcgc caatcgctgc ccgccgggca gcggaacacc
     3541 ctcgtcggcc tgcgtgtgcg ctggcgatgg aacgtagccc agcaaaggca ggcgcccgac
     3601 acccgcgagt ttcggatcta ctggcatccg ggcacggatc cgccggtggg ttgggagagc
     3661 gtggcaagct ggcagatccg atgcgacgtc aacgcgtacg ccagcaacgt gacggtcgcc
     3721 tccaatggcg accggagcta tgacgtcttg ctgccagcca ctgctgggca gggtcctttc
     3781 ggcaccggcg ttcctctaaa cccgacgcga gccaacccca tcgcgtacgc caatgtgacc
     3841 gtaacagcgg ccgacgacgc cgctcaggct gacacgtggc ccggtacggg tgcgctcgcc
     3901 gggaggacgg gcaacgagag ccgcgcggca tcggtgtcga aggtctatcg tgtcctgcgg
     3961 gaaccgccac cgccgcccga gccagtggtg gattccgatc gcctctactc gactccggcc
     4021 gactggcatg cgcaatccta ttacacgttt cggtggcggc cgggcgccga tgtctttacg
     4081 catgtcttcc gcgcgctcga cgagtcggtc ttcgccgccg attgggcact gcagccacgc
     4141 ccggacctgc agccgggcga tgccgcgcgc ttccccgacc ctgtcgcgga gcccacctgg
     4201 aatctcgcaa agcggcggac agtctgcacc gccctaaacg ccctcaatgc gttcccgaag
     4261 agcgatgcag gtaagcagca ggcgctgctg gcctaccgcg cactgtcgca cgacgcgctc
     4321 cgggtgctcg cgaacatgcc gggctgcgag aaggcattcg tgcaactcac cgtcaaggca
     4381 ctcgaccccg cggacgcggc taccgccgac cggcgcggac cggacgacgc actcggctat
     4441 gtcgtgcggc cggggctccg tgcatacact gacaagctcg acggacgagc caccaaccga
     4501 tatctctacc gcgcggtgta tgtcgatgcg gcgcagaatc gcagtgccat gggggccgtg
     4561 ggcacaccgg ttcgattgcc gaacgtcgtg cctccgcggg cgcctgtcct gaccaaggtc
     4621 accggcggcg accgtcgaat cacactctcc tgggcttcca atcgcgagcc cgatctgctg
     4681 gagtaccgcg tgttcagggc tgagacgaaa gacgatgccg gcgacttgcg gctgatgcag
     4741 caagtgggga cagtcgccgc ggatcccgat ccggcgctgc ggccggcggc ggagcagtgg
     4801 gacgactccc cagttcccgg cttgaaggat ttctggtacc gtatcgtggc ggtggaccgc
     4861 tccgacccgg ttgatccccg cggcggaggc ggaaatgtct cccagccgtc aagcattgtc
     4921 cgaggacggg cccaacaacc tcccccagca cccccgattg tgcagactgc cgtgtggaac
     4981 accggtcaca ctgcagtgca ggtcagctgg caaatcagcg atccagctct agtaccccgg
     5041 ctggagcgcc gccagaacga aggtgagccg tggctggtgg cggcaggttg gctggctccg
     5101 ggaaccctgg acgcgaccga cacgccccct gacccggcgc ttccctatca gtaccgtgtt
     5161 cgcgtgcgcg atcacgccgg ccagcaggcc gagagtcaac cggtggcaac ttcttagacg
     5221 cgattggagc cggatcatgc caaacgcctt acgattcctg ttcagtggca acgacctgca
     5281 aaaggtagtt ctgaactcca tccaggtcga ggacatcgtt gccggcgcgc cggcgggctc
     5341 cccgccaatc gcgtcgatgc ggctgaattt tcggcagagc gatccgggac cgctacagat
     5401 tcgtccgctg tttccgggga ccatgcgctt tctcgtcgac cccgcggcac ccggcgcgat
     5461 tccggatccg gcaaccggtt cgtttaccga ggccgattac cccggctgga aaacgctagg
     5521 gcggcttctc atcattctga gcgaccagac agccggcgaa atcgccggta tggcgcccat
     5581 gctgcccgtg aaaccgaacc ggatctggta tggtcccgtg cggctgcctc ccgggttcct
     5641 gttcgagacg ctgaccgcgg ggctgaagaa agctcccatt cctgcaagtg gagggcgaac
     5701 gatccgcacc tccaacgccg actggcgaaa gttcgcgatc gccgagtttc ttgctggccg
     5761 gtacaccccg cttttggtga catccgcgaa tgccgctcag gacgaccgcg tgcgcttcgt
     5821 catgcccacc gtggaggtac ctaacagcgg ggatatccaa ctcgacgtca cgacagcgct
     5881 tgcgcagaaa ccacaggatg cgttggatag cgaatttgag aacccggcgc ctccagtagg
     5941 gcaggaggaa ccaacgcatc cccggaatgg gttgattccc gccagggagg tctaccggcg
     6001 gcttcggcct ggttttgtcg ccgaaccagc ggctaccgcg ctgatgaacg caattctgtt
     6061 cgattggccc gagggaccgc tgttctggcc cattcaattc acccgtacct ccgaggcgac
     6121 aaacaactgc tccgtacact ttcccaggca aacggtgcac atgctcgcgg gcccgggtga
     6181 ttcgcaggac cagacactgc cggcgcacgg tttcgtatac ttccggcagg cgccccctcc
     6241 gaccggccaa ccggctcctc cagccccgat actgcaggtc tctttgaccg gtaatatgaa
     6301 gtggctgcca ggaggcggga cgagctggcg ctcacccggc cagaccgccg gtgtcgccat
     6361 cgacttcggc gctataggta cgccccacgt atcgcttcgt ctgccgatga gcgaagctat
     6421 gttttcggat acatccaggc ccgctccacg cggcgcgcgt tgcgcttatc tgtccatgcg
     6481 gcgaaccgtg cgcgcactga ttgataatcg cattgctggc gggcggctca atttcggagt
     6541 aaacaccact tcggccgtca ctcggggcat tatcaggcgc gccttccagg gaacgccggc
     6601 atcggtgaac gccgtagcgg ccaacgctcc gaatccggcg ggcgatccga acgttttggc
     6661 gctgactctc gaacccatgt ggcgcgcgtt tttcccggat cccgctccgc agcaaccgat
     6721 tccaggtgca ccggcaaatc cgaccgtgct cgacgagggc gagatggtct accggctttg
     6781 gcagtcgatt gccatcgcct tccagagcaa ccagacgagc cgaaatttct cggacgcgca
     6841 catcggccgc ggcgctcccg gcgcgatggt tgcagcaaac ctcgctgcct ttcatgtcga
     6901 tcccgtaaga aacgtcggcg acagtgacaa tgcatacttc gaccgcgtcg ccggctcgat
     6961 gctcgccggg ctccagcccg gcgcactgct ccagttctgg aacctgaaca gcgacttcga
     7021 ggacctcaag aaccgtagtg tccctggcgg agccaatccc caaattgcat cttatggcca
     7081 ctcgccagtg ttcgttgatt acgtactgga tgtgacagga aacgtaattg gaattacaat
     7141 tcttgaccag ttcggggaat ccacgtcggc agtggctggt cccgccggca accggcgcat
     7201 ccaatgggac ggcgacgacc agcagatatg gatcgccgct aactggtcgg agtgagttgt
     7261 gccatacgaa ataacaggca gagcgcttta cccgttatcc gaaatcctcc agcgtcagga
     7321 atttcctctg gcggagttga tcccgcagga tgcgctcgac accgtgttcg ggggcctcta
     7381 ctatacggac gccgaggcgt actccacatc cgaaggcctt cacttgaacc tgcggctcgc
     7441 cttcgaaggc gagctagccc tcagcccacc tgggttgcca ggtattgcgc tcgtggctgc
     7501 gtccggcggc atgggatgga cctcgctacg agcggaattc gtgattgggc cggaaccttc
     7561 tgccacgctc accgacgtgc cgctggtctt gcgcctcgat cgcagtctgt tgaaaccgat
     7621 gcggtcgcga acggaggtgg acgatagcaa acccggtgtg gacctcgcgc tgggcacggt
     7681 ggccctcaaa ttcagcccgg acggactaga aggcgaccta gacatctcgg tctcgcttcc
     7741 gctgagcatg attggcgata ccggtgttct catcgaagcc accaacgtgc gtccgattct
     7801 gggcccgatg aattcttctc tgcccggtgt cgacgcatcg ttccgcggcg ttttcatgga
     7861 gtcggtgcgc atttatcttc cggaaggact gcccgacttg gcgccgtcgg acctgcagat
     7921 cacgaactgc ttcattggta cgggcggctt cacaggagcg ctcgcgctca actacaaccc
     7981 ggtatacgat gcggcgacca agacattctc ggggcccggg tccggctcgc tcttcggcat
     8041 ggccctcggg ctcaccagac tatcgatccg attgacgcag aacacgttcg aggaagccgt
     8101 gattgccggc tcgatcgtcc tgccgttctt cgacgagctg ctcgatatcg agattgggct
     8161 cgctctggac ggcgggttca tggtagccat cgccagccca ggtggcctgg tcaccctcac
     8221 aaaaccggac ctactcgaac tttcgttgga gagcatccga ttctcactgg aggatggtgt
     8281 tttcaccgcg ggcgtgagcg gctccgtgca tcccctggtg ggcggaatcg agtggcccac
     8341 cttccgtgtg gaagagctct ccatcgattc ccagggccac gtccggatac agggcggctg
     8401 gctcgatctg cgcgagcaat acgtgctctc gctcggcggc ttccagttcg agatttcgcg
     8461 catcggattc ggcagcacag aagagggccg gcgctggatc gggttttcgg gcggcctgaa
     8521 gctcgtggac ggcattacgg ccggtgcctc ggtcgagggg ctgcgcgtgg cgtgggaccc
     8581 gaataccggc gatatttccg ttacgctgaa cggcgtcgac gtcgagttcc tcgttcccgg
     8641 cgcggtctac tttaaaggct atgtggcgat gacggagccg gcgccgggcg tcacccggtt
     8701 tgacggccag atcacgctcg acatcatatc ggtcggcctt ctgatcgaag cgcaactcgt
     8761 cgtcggctac gaccgggtga acgattaccc gttctttgcg atttatctcg gggtcgaact
     8821 gcccgcgggc attccgctag gccagacggg actcgggtta ttcggttttg ccgggctgtt
     8881 cgcgctgaac ctggaaccga accggcggcc cgatccagct cagccggaac tgccgtggta
     8941 cgggattcaa cctgggccaa gctggtacca tgcgccgccc agggtggggg tcgcggaact
     9001 ccggaagtgg gtcaatcggc aaggcagcct ggcgctcggc gcgggtgtaa ccataggaac
     9061 cgtatcggac aacggctacc ttttcgcggg aaagttcctg ctcggtatca tctttccggg
     9121 gccgattctg tttatcgaag ggcgcgcgaa cattctcaaa gagcgcgcca gcctgcgcga
     9181 tgaaccggtg ttccgcgcct tggtcgtgct cgatgcgcga gccggcacgt tcctgatggg
     9241 tctcgatgcc cgctacgcct atgatgacac cggcgccctc atcgagatcg gcggctgcga
     9301 ggcgttcttc gacttccacg acgtctcggc gtggcacatc tacatgggca tcgacgagcc
     9361 ccgcgagcga cgaatccgcg cctggatctt cgagcgcttg ttcgaggcca acgcgtattt
     9421 catgctcaat gccgagcgcc taaggactgg agcatggatc ggctacgaag cgcactggga
     9481 tttcgggccg cttgcggttg agctcgaggc gtggatcgag ggcaacgccg aactgagctt
     9541 caagccggtc tacttcaagg gcagcctgtg gctgcatggg aaaatcgccg cctcagtgtt
     9601 cggttttggc ttcgggctgg gggcggatgc gcgcatcagc gcggaagtct tcgaccctct
     9661 gcacattctg gcggagttgt cggtcagcgt gaacctacct tggccgctgc cggacttcga
     9721 tgcgtcgatc aagctggagt ggggtccaga gccggacccg ccgctactac ccgttccgct
     9781 caaagaggtc tcggtggagc acctgaaagt gaccacgaca tggccgctgc cggttaccgg
     9841 cggcacgatc ctgacgctgc ccgacccgga cggcgatggc gacgggtttt ttgacggtac
     9901 tgcccccgcg ccgccgtccg acacccagct cggaccctcc gacctttcgc cggttgtgcc
     9961 gctcgacgcg cggccgcgga tcacgttcgg caggagcgtc cacgatcttg ccacgattgg
    10021 cgagaacccg tcgccggtat ttcccgattc gcagccggag gccggctggg aatggatcgg
    10081 agatccggcg ctcaaccagg gaccggcgcg ggtacgcccc gccctcaccg agcttgcgct
    10141 cgagtgctgg aacggcacgc gatgggaacc cattgcccgt aaggccacta cttcaaaccc
    10201 cgtcggggtc cgcgctctct acggctcctg ggcgccggtg ccgcaactgc ccgcaggcaa
    10261 cgtgcagccc ggatcgccgc cgcctacagc caacgtcaag ctgtggctgt ggtcgaagtc
    10321 cccctatgat ttcagccgcc acaccggcgg ccagtgggat gagtggttcg atcacgcgta
    10381 tcccgactac ccctgccttc cgctccctcc agaccgtgag gactgctgtg atttctccgg
    10441 actgagcgct ggtgatatcc cggctccgcc ctgggggtgc agcgcgcatc cggagatcga
    10501 actcacctgg gcggcgccgc cgcgtccggt ggtgcgggtg aatgcggggc agaaggcgct
    10561 ttgctttccg aggggatccc atgtgacggt acaaatcggc gcgcgcacca agcgcgtgcg
    10621 cgtttccgtc cagtacgagg ccgggaagga aaccagggct tgtgcgaatt tcacagatga
    10681 ggctgtaggt gacggcgaga acccgcggcg cacgggggcc tttcgcttcg tcgtgtacga
    10741 tgcggccggc caactgctgc cgcgcaccag ggtgatccaa gtggcaacca gcacgggcgg
    10801 tcggcggggc ctggatgcgg gattccgcgc caccatcgac ttgccggcgt cggacttcgt
    10861 ggagctggac ttgacacggt taaaccagcc tgcgaggctc caagctattg ccgctggcgg
    10921 gggcctgatc gctgagacgg tgatgtcggc cgcaatcggc gcctcgcaag tggttcggct
    10981 cgagcgcgtc cgaggtcagc gcgagatcgt ccgggtgacg gtaacctcgc cggcggaccg
    11041 ggttgccatc catagcgtgt gctacggcgt ccggccacgc agcatcgtgg gccgtgcgat
    11101 cgaccggagt gggagagagc tgggcgccta ccgggatgtc aacgggctga tcgacgtccc
    11161 gggacgggac gtggttgcgg tgcgtctcga tgcggacgga ggcgccttct gcgtcacgcg
    11221 aatctgcgtg accgtcggcc tggaccaggc cgaccgcatt cagcgcgaag agatgctgcg
    11281 ccacatgatg gacgaactgg tccgctggga ggcagatgac gaggtgctgg cgccgtggac
    11341 ccggtatcgg ctcaagatcg tgactaccct ggatgtccgc ggcttctcct atgacgcggc
    11401 gttcaattcc acccggacga tcacgcagtg cgcctacttc cgcaccgagg gcccgcccgg
    11461 attgactacg tattccgtgc ccatcggaca tccccaggaa aacgccgccg cgcccgcggg
    11521 agcaagcgac ccgccgcagt tcgacagcgg attgcaggat ctttcgcggt acgttgccca
    11581 gaccgtgccg ccgaccgtgc ccgctgccgg cgagaagccg cctctgccga agcctgtcta
    11641 ccgcgcctac gatgtcgggg tgcggttcaa cgaaaactat gtggaaacga tgtatcgcct
    11701 ggccggccgc gatctgacgc tcgcaattta cgacaacaac aacgagccgg tgcgcgatca
    11761 tcgcggccgc ctcctcattt ctccgaaccg ctgggggcgc gccgatacgg tcgatcttgc
    11821 cgcctccgag gagcgatgga tctcccacat caatgaaacg gcctgcttgc aactcgaccg
    11881 cacacgcatc gcgcgcgacg tcacgcttgg tgccgacacc catgtgctcg atccccagac
    11941 cgtgtacgaa gcgcgcctgc tgccgctgtt gatgcacgac gctttcgcgg attacacggc
    12001 cggcgcacag gcgcgcggcg caggagctac gctcgtgccc acaaccggcg cgggctggac
    12061 ggtccgcgat gagggaaccg accaggggcc gtcgcagtgg atcgttcgtg aggcgggcaa
    12121 tccgccatca cgctatgttg agcagacgac caacgtatcc gccggcgcgg cgcggcgatc
    12181 ttccgtattc gcggggggca cgctgctgat ttgcgccgcc gatccgcgcg ccggcgtgcc
    12241 gcaagccgac cagcctgcga actggacgga ctaccgcctc tcggtctata tacgatcgct
    12301 cgacgatgac attgtcggcg ccggagtgcg ctggtcggga gcaagcggtt atctcttgac
    12361 tttggaccgg gaactcaacc ggcggcggct cctcagactg gtgaacggcg cagcaacggt
    12421 gctggacgag tcggcgggcg gctatgacac gaaccgcgat tacctcgcca gcatcgaggc
    12481 tgctggacct cgcatccgcg tgtttctgga cggggcgcca ctgttcgacg tctccgataa
    12541 ccggttcgca tcggggagcg tggcgctgta ctgcggacag aacgccggcg gccacttccg
    12601 cgacgcccgc gtggacgacc tgcgcggcac ggcgcctgtg gtttaccgcc tcaagttcac
    12661 gacctccgac ttcaccgact ttcgccacca tatccacagc tttccgagcc aggtgttccg
    12721 tgccgcgttt cccgacctca acgatgtcgc ggcatccatc ggtgcggcgc tgccgctgac
    12781 cggcgcgacc caagcgccgc aggaagcgga agcacgcgcc tacgacacgc ttgccacgaa
    12841 ggcgctcggt accgcggcgc ggcagccggt cgcgctgctg gatgtgaccc gggtcgagca
    12901 gggtggagcg gcgttgggat tcttggtgcg tacagccgat ccgattgact ggagacgcac
    12961 cgcgctgtcc ctgctgcggg cgccgcagca gccattgatg cccgatgcgc ccagcattcc
    13021 gcggttgatc cgcgcaacat tcgcgacggg cgccacgccc cagccgaacg acgaatcgat
    13081 aactgtgttg ctcgacaccg gtatggatct agctggatgg cagatccaac gccgaaccct
    13141 gccgagcgcc aatgcgcagg ccctggccgc ggaaaccgtg ctgttccggg ccaacctcga
    13201 cacggaccag gggccggact ccatccaatc ggactctctc tggaaaccgc ggctgaacaa
    13261 tctaaacgac tttgacatgg cggattctcc gggaggcatc ggcacatcga tgtgggcggc
    13321 cggggcgggc ttcttgcggc aggacggcaa tttccgcgtc acggatccgc ctttgaccga
    13381 accgccgcct cgcttgggca cttatgccgt gcgtgccctt gccggagagt ggcgtgatgt
    13441 ccgcttctca gttcgcctgc gggccaacac cggcgcggcc gcgggagtgg tcttccgcta
    13501 ccaggacgtt cagaaccatt accgcttcgc cttcgacagc gcgcgcaacg tgcaggagtt
    13561 gaccaagcgt gtgaacggca ggaccagcct gctcttcagg aagcctttta atttcgccgc
    13621 aggccgcttc catgcgctgg tcgtggaggc tctgggcgca agactgcgag tcactgtcga
    13681 cagactgcag atcgccgagg tgttcgacga cgatctggcg caaggtgccg ccggcttcta
    13741 cacgttcctg caaccgctgc cgcgcttcga ccaggtgaca gcggcatcgc tcacgcgaac
    13801 gctcggcccc tggacgatct acgatcatgg cgacgtgcgc gcgacttcgg agtggcgcgt
    13861 ggagaaccgg ctgctacggc agagcgtcga tcttttcacg gcgccgccag cggctgccac
    13921 tgcccagcgc tcgggcagcg cggcagtaac cggcgatgcc gcgtggaccg acgttcgcat
    13981 tactgcggcg gcgcagccgg aggccgcggg gatagccggc atcgtgttcc gctggcggga
    14041 ttcggataac cattaccggc tcgtgtttga tggggtcaac gggcagcggt ggctggtcag
    14101 gcggatgggc ggagtctcta cgagactgtg gtccggcgcg gggcagctcg gcacgatgga
    14161 agttgccgtc gaggcgatcg gcaacaggct gcgcgcctgg gtcgatggcg cggtactctt
    14221 cgacgtgtac gacgatgccc tggcagccgg ccgggccggt gttctgtcga tggatgacag
    14281 cgcgggcgtc tggtccgctt tcgaggtccg gcatgtgcag cccgcgtggg aggactggca
    14341 tgtcttcggc accaacgagg gttggcgcgc cgctggccgc cgcttcgaaa tccgcgccgg
    14401 ccgcagcgcc gactcggcag cgcctgcatc cggagcgggc gaggagcggc tgtttcagga
    14461 attggcctct gccggattcc agccgcggct ccgcgcgccc ggcgtcgacc tccgtcttgt
    14521 ggggaacgat ggcgagccgg gacacatgcg ccgctttctt cccgacggag cgtacgttgc
    14581 gcaggccact gcacgcattc tccgatcagc cgacggcacg ggtatgtttg tgtttgtgcc
    14641 cggagctgga ccggccggtg ccggccttcc atccggcgaa taccgcctcg agttcaccta
    14701 ccgccgcgat aataccaacg ccgatccggc tagtctggtc ctttcccagg ccagcgatac
    14761 ggcggacgag acggcatggc tcgacatacc ctggcctgct cactgagcgg tcagcccgct
    14821 cacggctttc gcatttggat tggtacggct gatcgggatc ggccggttcc cggccaattg
    14881 cctcagcgcc tcacaggagt gtaaccagca gccggtgcgg cacgactcca cagaattggc
    14941 accttggtcg gagcactctg cgattgctgg tcgaacagac ttagcatctg aggctgctcc
    15001 ggttctggct tggcgggcgc cggtggcgtt gctggcagcg aatcagtcga cttcttcccg
    15061 ccgttaccga cgaccttgtg cttcttcctt gcttcctccc gcgctgcttt ctccgcctcg
    15121 gcaatttcct tctcgatcgc cgccaggttg gtattcaggc cgacgtggga agccacgaag
    15181 ttggagacct gacacaccag ctcggcgtcg agttcggcgg ccgtgccggt cacgcagagc
    15241 ggaattgtca gtgctgcgtt ctcgccgtcc ttcaactgcc gcggacagat attcacctga
    15301 agctgatccc cttcaagttt cgacacggtg atgagcacgc gcgatgggcg agcaacggct
    15361 ggagttgact gaagagtcct tgaggctgtg tcgttggtgt catacgattg gctccctata
    15421 gagcgggcca ccgagaacgc ggcggcccgc gatgatcaga acggttttac gcggatttcg
    15481 aagtgttcgg attgataagc ggtctgcgga ccgttcactt tcaggggtgt tcggttgaac
    15541 gcccggtttc agttcagccc tcgatgtgga cgctttttgc gccgttgatg taggcggtat
    15601 agccgaccga acagcggtgt cagaactaac gtcagcagac ccgcgattac gaagatggca
    15661 atgccgatcg tgcgcaatag ggatgccaac gcgaagccga cgacagcatc cttccacgcc
    15721 cccggatcgg tctgaacgag tagctttgga tctgtcgcgc tgagctcccc gccgtcgcgc
    15781 cgaatgtcga ggacaaccga gtagtgctga ccctttcggg cggcaaaacg tccaatctcg
    15841 cgcgctactg tgccgctata gaatccaccc caagaattcc gcgaggatcc gtttgccatc
    15901 accagaccgt cgtgcagaac gctccaatcg atgtctatca tgtcgggggc atctgcgcag
    15961 gtttgagccc gccgctagtc aaccattccc agcatgcatt caagcctttt gaactcgatc
    16021 cggcgttcgc tctcaaccag cagacggtag ttcgtatcaa gcccggcttc aaatacatct
    16081 gtgtggactc tgccaacctt gagctggatg ggcacagcga tgggtttcca ccgccaaatg
    16141 tcgacggggg cgggatggat acaatagacg acacccgcta gcgccagtag aacgagggca
    16201 acgtactggt gccgcttcgg tcgatatctc atccgttctt aaagtacgcc atttggggac
    16261 agtttgttct ggacacgccg agaatcgggg accattgcgg gctcatctgc gccagttgct
    16321 gtgttgaaat tacccctatt ccaggtcggg caactcttcc cgagggatta gcagccgaat
    16381 cgaccgaccc tgcccaggca cccgcgcgat caagccattg gcctccagcg tgaccaccat
    16441 ctggtgtacc gacggcggcg acaccttgaa atacttttgc atgtcggatt cggctggagc
    16501 gaacccgtgg attttcgtgt agtagaaaat aaacgccagg tactgtcctt gcttcgcggt
    16561 gtagggtctc ct
//
LOCUS       DASTMK010000109        14252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_549275, whole genome shotgun sequence.
ACCESSION   DASTMK010000109 DASTMK010000000
VERSION     DASTMK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549275"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /gene="ligD"
                     /locus_tag="VFQ79_04390"
     CDS             complement(<1..2538)
                     /gene="ligD"
                     /locus_tag="VFQ79_04390"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEU0138921.1"
                     /translation="MSLKEYVRKRKFDQTPEPKPSAATPPVASRFFVQRHDATRLHYD
                     FRLEMFGVLKSWAVPKGPSLDPGVKSLAVHVEDHPLDYGTFEGNIPKGNYGAGSVMLW
                     DQGMYELLGDTSAADQYERGDVKFRLMGEKLRGTFALVRMKNRGKGNEWLLIKKRDEE
                     AVPGWDIEQYAWSVSTGRGQEEIAKDLPSVNRKKSRKRSTASSKNGPATLAGAVAAAM
                     PESVTPMLASLASAPPEGKQWIFEVKWDGVRAICYVDGGKMRIVSRNGLSFNRQYPEL
                     SVIPHRVQAASFILDGEIAALDEQGRSRFELIQSRIMVANANAVAHLSRKTPVFFFAF
                     DLLYWDGYDLRRVPLLERKKALEALIQPGECLRYSQHFQVPGAEIVEAARQMGIEGVI
                     AKQADSLYEGKRSSKWLKIKIVNEQDAVICGFTHGEREFFSSLILGIYDKNRLTYAGN
                     VGTGFDDKLIKKIYEQLSPLITETCPFRPKPKMIRKASWVSPELVCRVKYTEWTAEGR
                     LRAPVFLGLRSDIDPHQCVRESPGNNSAESAAEPKRERPLLRPGEEEVTRAIDGRSIK
                     FTNLNKLYYPDDGYTKRDVINYYDAVAPLILPHLRDRPLSLKRYPNGIRGEFFFQKDI
                     PATYPSWLRIEPIHSEHRNAPIRYVVADDRATLLYLANLGCIDQNPWMSRIGTLDYPD
                     FILIDLDPLECSYDQIVEAARITKSKLDLLGLEGVPKTTGGDGMHVYVPLEAKYTYEQ
                     VRSFAELLSHLVVEENPDLFTTPRAVTKRRKGRVYFDYLQNSSSKTIAAPYVLRAHPK
                     APVSTPLRWSEVKHGLRPEQFTIANAVQRFDRVGDLFAPVLENRQTLE"
     gene            complement(2554..3135)
                     /locus_tag="VFQ79_04395"
     CDS             complement(2554..3135)
                     /locus_tag="VFQ79_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138922.1"
                     /translation="MFLFIEQGGRLLQKGTREGLKGPFWGDYFRSVKNLGGWNDGDTV
                     MANFVGHPMQGAVSGYIQVQNDDFGKYQEFGLEPDYWRSRMRAMFFSAAYSLNFELGP
                     ISEASIGNVGKKPGTMGAIDLVFTPIGGLGWQITEDAMDRFFIRWVERKTDNLGVLII
                     TRSMLNPSRAFANILRFRVPWHRDTRPGITFRR"
     gene            3272..4531
                     /gene="tyrS"
                     /locus_tag="VFQ79_04400"
     CDS             3272..4531
                     /gene="tyrS"
                     /locus_tag="VFQ79_04400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU0138923.1"
                     /translation="MTQRSPAIEHSLISYNEVVSFPSVDEQVIYLKKGLAELIREDEL
                     RDRLKRSRETGKPLLVKVGFDPTAPDLHLGHTVLLRKMKHFQDCGHTVIFVIGVMTGL
                     IGDPTGRNITRPPMTREEIDQNAETYKAQVFKVLDRERTEIRYNSEWLEPLRLEDVIR
                     LASKYTVARLLERDDFSKRFKEGIPISVHELFYPLAQAYDSVALACDVEMGGNDQKFN
                     LLVGREIQRDYGQPPQIVATTPLLEGLDGVEKMSKSKGNYVGITESPEEMFKKLMSIS
                     DDTMFRYYELLTDTTMVELDAMRRRIAGGSLHPKQAKIDLAHQVISDFHSRDDADRAA
                     AEWQRVVASGEVPSDIETVRMTGPAVRIDKMLAKAGLADSVSDAVRKIKAGAVEVNGS
                     RVGDLIQLEFPATYVVRVGKRWKQITA"
     gene            4552..5370
                     /locus_tag="VFQ79_04405"
     CDS             4552..5370
                     /locus_tag="VFQ79_04405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0138924.1"
                     /translation="MNDILYLQPTDVPEAMHLSRAAGWNQTEDDWQRVISIAPDGCFG
                     IRTEGRLVATATAVCYGKELAWIGMVLTGPDYRGRGYARRLMKQALEYLAGRDVAWIK
                     LDATDMGRPLYSSLGFEDECLVERWWRGGTEGTSGGLSFNPILPLELDGAAFGTDRSD
                     LLAKLSPYGCAAGENGYAMWRPGEKALYFGPCVARQEEEGRRLFDGFLAMHGGQDIFW
                     DLLPGNSAAVQLASAAGFECRRRLARMTLKGRGNARPLGNNDQYVYAIAGFEYG"
     gene            5387..6232
                     /gene="folP"
                     /locus_tag="VFQ79_04410"
     CDS             5387..6232
                     /gene="folP"
                     /locus_tag="VFQ79_04410"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU0138925.1"
                     /translation="MTRKRYSWKLKNREIQLGEKTLIVGILNVTPDSFSDGGRYTDPD
                     RAFARAVEIEEQGADIIDIGAESTRPGSARISESEEMGRLVPVLKRLRDKLGIPISVD
                     TYKAAVAERALELGAEIINDPTGLTFDPHLAKVVMNRDAGLILNHMRGTPDTWEKLAP
                     LKDVMGTIAMEMEATVSRARLAGMDRTRIVIDPGLGFGKRKEQNAEILAQLHALARFD
                     LPLLVAPSRKSFLAQKTEQGTAYATAAAVTAAILNGAHLVRVHDVVEMAVVARVADAV
                     LEYSG"
     gene            complement(6229..7020)
                     /gene="mutM"
                     /locus_tag="VFQ79_04415"
     CDS             complement(6229..7020)
                     /gene="mutM"
                     /locus_tag="VFQ79_04415"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU0138926.1"
                     /translation="MPELPEIETIVRELAPALAGRLIRDVEFLSRLILLDSAQAGIDF
                     LRGRRMVSLIRQGKFIVCRLDRGFLTFHLGMTGKLLLDSGKGPYTRAVFALDSGSLRF
                     DDIRQFGRIEWGECVPARVERLGADPLSISASEFIRQVRRHSGHIKPLLLNQTFLAGL
                     GNIYTDEVLHRAGIHPRTRATRLGRERCLRLHRVIVEVLSLAIEHRGSSISDYVDASG
                     RQGSFQLLHQVYGKPGQPCPRCGTPIRRIVIAQRGTHFCPDCQKH"
     gene            complement(7021..7326)
                     /locus_tag="VFQ79_04420"
     CDS             complement(7021..7326)
                     /locus_tag="VFQ79_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEU0138927.1"
                     /translation="MALVRVGPAAALPSGSMLQVDTDCGVYAVCNIDGEIHAVDGICP
                     HRGGLLAHGALHGAAIVCPWHAWEFDCRSGEYDFDPAIRLKKHSVAIEDDIIFVDLP"
     gene            7395..8303
                     /locus_tag="VFQ79_04425"
     CDS             7395..8303
                     /locus_tag="VFQ79_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEU0138928.1"
                     /translation="MHIPDGFLSTPVWLTMNAISVPAVGVLARRAHSHLDDSRIPLLG
                     VMGAFVFAAQMINFPVGAGTSGHLVGGALLACTLGPAAATVVMTAILAVQALVFQDGG
                     VLSLGANVFNMAVLGVLAGYLPYHFWGAGRLRKFSFFAGGVLSVLVSAALTVSQLLIS
                     GIPMRTGVLAISAGLFLISALIEGLITVAVIGALEKLNPNWIHKPMSEGRLGMGLLAS
                     AAVLLAVVGVVVASAAPDGLESLAEQIGLADKATVLFSTPLADYEANFLNSDWFRKAA
                     AGMAGLMLIFGVCILCGRMISRRRSA"
     gene            8315..9031
                     /locus_tag="VFQ79_04430"
     CDS             8315..9031
                     /locus_tag="VFQ79_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU0138929.1"
                     /translation="MVLDRWSRRTSPLHRRDARAKILAALALLISIATTDAGMLPMLA
                     AYGAIVAAGFLAARLPLFQAAARAGVVLPFSLAMALVSWVSGDPARAASVVVKSYLSA
                     SIVLLLIATTPLTELLSGAEKLGAPRVVTLVVQFLYRYLFVISEQAQHMLQAARCRSG
                     SRNRGTRRRFQAAAGALAVLFSKSYIRAEGIHNAMLARGFQGRLPAIRVQRFELADFG
                     FLLAALLVAAGTGLALGGIA"
     gene            9031..9729
                     /locus_tag="VFQ79_04435"
     CDS             9031..9729
                     /locus_tag="VFQ79_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0138930.1"
                     /translation="MKTVIEVRNLRFRYSDGTVALDGVDFDLYSGETVALLGANGSGK
                     TTFILHLNGLLSGEGSVRVCGIPVEKKTLPLIRQKIGLLFQDSDEQLFMPTVLEDVAF
                     GPMNAGLSRDEAITKAGKALHQTGMAHVSDKAPYHLSAGEKKRVALAGVLAMEPEILV
                     LDEPTTYLDPPGQRELLRLITGLPQPKIVVTHDIGFASRMGARAVFFEKGRIVETGTL
                     RDIVRRFHWEVSSD"
     gene            complement(9749..10084)
                     /locus_tag="VFQ79_04440"
     CDS             complement(9749..10084)
                     /locus_tag="VFQ79_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138931.1"
                     /translation="MARKSSVNDISLLEAALEGLELQRKRIDEQITHVRSLLGKRPGV
                     KSSVSSVPVSTGQKRKLSPAARRRIAAAQKKRWAEYRKNAASNSSRKPRVRPDRRAVS
                     AVKSASEAA"
     gene            complement(10116..10580)
                     /gene="dtd"
                     /locus_tag="VFQ79_04445"
     CDS             complement(10116..10580)
                     /gene="dtd"
                     /locus_tag="VFQ79_04445"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU0138932.1"
                     /translation="MAAYMIMVLQRAGSSHVQVGADTVGSIESGLVVLLGVAKGDTRK
                     DADYLVEKALSLRIFPDETGKMNKNIQQAGGSLLVVSQFTLLGDCRKGRRPSFDRAAD
                     PQQAKELYEYFVSAARASGIRVETGIFQAFMSLRLTNEGPVTIICESHGNNK"
     gene            10621..11664
                     /locus_tag="VFQ79_04450"
     CDS             10621..11664
                     /locus_tag="VFQ79_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEU0138933.1"
                     /translation="MIECYAYARAGLLGNPSDGYFGKTISFLVRNFRARVLLYPSARL
                     EIRPSKADMPVFENLEDLYKTTRWRGYYGGIRIIQALLVRFLDYCAEQGIEIEDRNFT
                     LEYETNIPLRLGMGGSSSIITAALRAIMQYYGIAIPLPVQAKLILETETRELGVPAGL
                     QDRVIQVYEGLVYMDFSRELMERQGHGNYEKLDPACLPDMYVAYRTSLSEGTEVFHND
                     VRERWRRGDPEVTGAMQEWASIAERGKTAILNRDYGLLDELINRNFDLRATLYRISPG
                     NLEMVETARRAGASANFAGSGGAIVGSYRGEDMFRQLTAAMQSIGVAVIKPRIDAGRT
                     NHIDLRSCADSAR"
     gene            complement(11676..12929)
                     /gene="icd"
                     /locus_tag="VFQ79_04455"
     CDS             complement(11676..12929)
                     /gene="icd"
                     /locus_tag="VFQ79_04455"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HEU0138934.1"
                     /translation="MRSYNNIPVPDGGAPIQVQDEKLVIPDNPVIPFVEGDGTGRDIW
                     RASRKVFDAAVERAYSGKRRVYWYEVFAGEKAFNQFNNWLPQDTLDAIQQFHVAIKGP
                     LTTPVGGGIRSINVALRQLMNLYVCMRPVRYYPGVPSPVKSPEKLDVVIFRENTEDVY
                     AGIEWREGTGEAHRVIEFLNTQMGTKIRTDSGIGIKPISVTGSKRLIRSAIRYAIENG
                     RRSVTLVHKGNIMKFTEGAFRDWGYELVKEEFSDTCITEADLGSSAPNGKIVVKDRIA
                     DSMFQQVLLRPDEYDVIATPNLNGDYLSDACAAQVGGLGMAPGGNIGDEHAVFEATHG
                     TAPKYADQDVINPSSLILSGSMMFRFMGWREASDLIEKGITGTIREKRVTYDLHRLME
                     GAVKVKTSEFADAIIENMSGRSVAA"
     gene            13064..14167
                     /gene="gcvT"
                     /locus_tag="VFQ79_04460"
     CDS             13064..14167
                     /gene="gcvT"
                     /locus_tag="VFQ79_04460"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU0138935.1"
                     /translation="MNPAVSLKTTPLNEIHRKLGAKMVDFGGWDMPVQYTSILEEHHA
                     VRRAVGLFDVSHMGEIEVRGPEALNLVEYVTTNSASRLEIGQAQYSGLLYRHGGFVDD
                     VLVHKVDDDHYFLCVNASNQEKDHEHISIANSWKTNVEFASDRYAQMAIQGPKALATL
                     QKLTPVKLDGIRNYHFTDGEVSGVPARIARTGYTGEDGFEIYVAPDEAPAIWAELLVA
                     GEEFGIRPCGLGARNTLRLEAKMALYGHEIHASITPYEADLGWIVKLDKPDFIGKQAL
                     EKQKAEGIRRKLVGFEMRGRGIGRDGYEVLIDGLPAGWVTSGGPSPTLNKNIGLCYLP
                     AELAAPGRTIHILIRNQPVEAITVATPFYKRAK"
     gene            14171..>14252
                     /locus_tag="VFQ79_04465"
     CDS             14171..>14252
                     /locus_tag="VFQ79_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEU0138936.1"
                     /translation="MYPENLRYTKEHEWVLVEGDTATVGIT"
BASE COUNT         2916 a         4132 c         4270 g         2934 t
ORIGIN      
        1 ttccagcgtc tgtctatttt cgagaacggg ggcgaagaga tcgcccactc gatcaaaacg
       61 ctgaacggcg tttgcgatcg tgaactgctc cggccgcagt ccgtgcttca cttcactcca
      121 gcgcagcggc gtcgacacgg gagctttcgg atgtgcacga agtacgtaag gagccgcaat
      181 ggttttcgat gacgaattct gcaaatagtc gaagtaaacc cgtcctttgc ggcgcttggt
      241 cacagcccgg ggcgtggtga acaggtccgg attttcctcg actacaagat gcgacagcag
      301 ttcggcaaac gatcgcacct gttcgtaggt gtactttgcc tcgagaggga catagacatg
      361 cataccgtca ccgccggtgg tcttcggaac gccttcgagt ccgagaaggt ccagcttcga
      421 ttttgtaatg cgcgcggctt ccacaatctg atcgtaagag cactccagcg gatccaggtc
      481 aataagaatg aagtccggat aatcgagcgt cccgatccgg ctcatccatg gattctgatc
      541 gatgcaccca agattggcga gatacaacag cgtggcccga tcgtccgcca ctacgtaacg
      601 gatgggcgcg ttgcggtgct ccgagtggat tggctcgatt cggagccacg acggataagt
      661 agccggaatg tccttctgga agaagaactc gccgcgaata ccgttagggt agcgtttcaa
      721 tgaaagcggc cggtctctga ggtggggcag aatgagaggc gccacggcat cgtagtaatt
      781 gatcacgtcc cgttttgtat atccgtcgtc gggatagtaa agcttgttca ggttcgtgaa
      841 cttgatggat cggccgtcaa tcgcgcgcgt cacttcctct tcgccggggc gcagcagagg
      901 acgctcccgt ttcggctcgg cagcgctttc agcactgttg ttgccgggcg actcgcgcac
      961 gcactggtgg ggatcgatgt cggagcgcaa tcccagaaaa acgggtgcgc gcagcctgcc
     1021 ctctgccgtc cattccgtgt attttacgcg gcataccagc tcggggctga cccaactggc
     1081 tttgcggatc atcttgggtt tggggcggaa ggggcaggtc tccgtaatca gcgggctgag
     1141 ttgctcgtag atttttttga tgagcttgtc gtcgaagccg gtaccgacat taccggcgta
     1201 cgtcagccgg tttttatcgt atataccgag gatcagtgag ctgaaaaatt cacgctcgcc
     1261 gtgtgtgaaa ccgcagatga cggcgtcctg ctcattcacg atcttgatct tcagccactt
     1321 cgagctccgc ttcccttcgt acaggctgtc ggcctgcttg gcgatgactc cttcgatccc
     1381 catctggcgg gcggcttcca cgatctcggc cccgggcacc tgaaaatgct gcgagtaccg
     1441 tagacactcg ccgggttgta ttaaagcttc gagcgctttc ttgcgttcga gcagagggac
     1501 gcgccggaga tcgtagccat cccaataaag aagatcgaag gcaaagaaaa ataccggggt
     1561 cttgcgggaa aggtgggcga cagcattcgc attcgccacc ataatgcgag actgaatcag
     1621 ctcaaagcgg gagcgtccct gttcatccag cgcggcgatt tcgccatcca ggatgaagct
     1681 tgcggcttgg acacggtggg ggatgacgct caattccgga tactggcggt tgaacgacag
     1741 cccgttgcgt gagacgattc gcatcttacc gccatcgaca tagcagattg cgcgcacacc
     1801 atcccatttc acttcgaata tccactgctt cccttcgggc ggcgcggagg ccagggacgc
     1861 aagcatgggt gtaaccgatt caggcatggc ggcggctacc gcccctgcaa gcgtggctgg
     1921 cccgttcttc gaactcgcgg tcgaacgttt gcggctcttc tttctgttta ccgaaggcag
     1981 atctttggca atctcctcct ggccgcgtcc ggtcgacacg ctccatgcat actgctcgat
     2041 atcccagccc ggaacagctt cttcatcgcg cttcttgatg agtagccatt cgttcccttt
     2101 accgcgattt ttcattcgca ccagggcgaa agtgccgcgt agtttctcgc ccatcaggcg
     2161 aaatttcaca tcgccgcgtt catactgatc ggccgcggag gtgtcgccca gcagctcgta
     2221 catcccctgg tcccataaca tcacgctacc cgcgccgtag ttccctttgg ggatgtttcc
     2281 ctcgaaagta ccgtagtcga gcggatgatc ttccacatgc acggcgagag actttactcc
     2341 cgggtcgagg gacggacctt tgggaactgc ccaggacttg agcacgccga acatttccaa
     2401 ccggaagtcg taatgcaggc gggtggcgtc gtggcgctgg acgaaaaacc ggctggccac
     2461 cgggggcgtg gccgccgacg gcttcggttc gggagtctga tcgaacttgc gtttcctgac
     2521 gtattccttg agggacataa ccgaattggg gcactaccgc cggaaagtaa ttcccggccg
     2581 cgtgtcccgg tgccagggta cccggaatcg caggatattg gcaaacgcgc ggcttggatt
     2641 aagcatcgaa cgggtgatga tcagaacgcc aaggttgtct gtcttccgct ctacccaccg
     2701 gataaaaaag cggtccatcg cgtcctcggt gatctgccag ccgagccccc cgatgggcgt
     2761 gaagaccagg tcgatggctc ccatggtgcc cggtttcttg ccgacattcc cgatcgatgc
     2821 ctcgctgatg ggacccaact cgaagttgag actataggcg gccgaaaaaa acattgcccg
     2881 catcctgctt ctccaataat ccggctccag cccgaactcc tggtattttc cgaagtcgtc
     2941 gttctgtacc tgaatatagc cggataccgc cccctgcatc gggtgcccga cgaaattggc
     3001 cattacggtg tccccgtcgt tccatccgcc aagatttttg acggatcgga aatagtcgcc
     3061 ccaaaagggg cctttcagcc cttcccgcgt acctttctgg agaagccggc cgccttgttc
     3121 gataaataaa aacatcgtgg cctgccttag ggcgggcccc caatgaaaac ctggctccgg
     3181 ttccgaatcg ccttgagcgg ccacagtaga ggggatcata accagaagac aggaaattgc
     3241 aactgttttc ctcattgatc ttccatagaa aatgacgcag cgaagccccg ctattgaaca
     3301 cagtttaatc tcctacaatg aagtggtgag tttcccctcc gtcgatgaac aggtgatcta
     3361 tctcaagaag gggctggccg agctgattcg tgaagacgag ctgcgcgacc ggttgaaacg
     3421 aagccgcgag accggaaagc ccttactggt aaaggtggga ttcgatccga ccgcccccga
     3481 tctgcacctg ggccataccg tgctgctgcg aaagatgaaa cattttcagg actgcggcca
     3541 tacggtgatc ttcgtaatag gcgtcatgac agggctcatc ggcgacccga ccggacgcaa
     3601 tatcacccgt ccaccgatga cacgcgagga gattgatcag aacgccgaaa cctacaaagc
     3661 acaggtattc aaggtcctgg atcgcgagag aaccgagatt cgatacaaca gcgaatggct
     3721 ggagccgctg cggctggagg acgtaatccg gctggcgtca aagtataccg tggcgcggct
     3781 gctcgaacgc gacgattttt cgaaacgttt caaagaaggc attccgatct ccgtgcatga
     3841 actgttctat ccgctcgccc aggcttatga ctccgtggcg ctcgcctgcg acgtggaaat
     3901 ggggggcaac gaccagaagt tcaatcttct ggtcggccgc gaaatccagc gcgattacgg
     3961 acagccgccg cagatcgtcg ccaccacccc tcttctcgaa ggactggatg gcgtcgaaaa
     4021 gatgtcgaaa tcaaagggca attacgtcgg catcacggaa tcgccggaag agatgttcaa
     4081 gaaactgatg agcatcagcg acgacaccat gttccgctac tacgagttac tgacggacac
     4141 caccatggtg gagctcgacg ccatgcggcg gcgcattgcc ggcggcagtc tgcatccgaa
     4201 acaggcaaaa atcgacctgg cacaccaggt aatttccgat ttccattcgc gtgacgatgc
     4261 ggaccgtgcg gccgccgaat ggcagcgtgt cgtagcctcc ggcgaagttc cttccgacat
     4321 cgagaccgtc aggatgacgg ggccggcggt acgcatcgac aaaatgctcg cgaaggcggg
     4381 tctggcggac tcggtcagcg atgccgtacg caagatcaag gcgggagccg tggaagtcaa
     4441 cggcagccgc gtgggagacc tgatccaact cgagtttccc gcgacctacg tcgtgcgtgt
     4501 cggcaaaaga tggaaacaga tcacggctta gcccttctcc acccgggaac catgaacgat
     4561 atactctatt tgcagccaac cgatgtgccg gaagcgatgc acctgagccg tgctgccgga
     4621 tggaaccaga cggaggacga ctggcagcgg gtgatttcca tcgcgcccga tggctgtttc
     4681 ggcattcgca ccgaaggacg gcttgtggcc accgcgaccg cggtctgtta cggcaaggaa
     4741 ctggcgtgga tcggcatggt gctgaccggc ccggattacc gcgggcgcgg ctatgcacgc
     4801 cgcctgatga aacaggcgct cgaatatctg gcggggcgcg atgtcgcctg gatcaagctg
     4861 gatgcaaccg acatggggcg tccgctctat tcgagcctcg gatttgaaga cgagtgcctg
     4921 gtggagcgct ggtggcgggg tgggacggag ggaacctccg gcggtttgtc cttcaatccg
     4981 atattgccgc tggagttgga cggcgccgcg ttcgggacgg accgttcaga ccttttggcg
     5041 aaactctctc cttacggctg tgcggcaggc gagaacggct acgcgatgtg gcgtccggga
     5101 gagaaggcgc tctatttcgg accctgcgtt gcccggcagg aagaggaggg acggaggctg
     5161 ttcgacggct tcctcgccat gcacggcggc caggatattt tctgggacct gctccccggc
     5221 aatagcgcgg cggtccaact cgcttccgcc gcgggattcg agtgccggcg ccggctggcg
     5281 cgcatgacgt tgaagggtcg cggcaacgcc cgcccgctcg gaaacaacga ccagtatgtc
     5341 tatgccatag cgggtttcga atacggttaa aaatgagcgc cctaccatga cacgcaaacg
     5401 gtattcctgg aagctcaaga accgcgaaat ccaattgggc gagaagaccc tgatcgttgg
     5461 gatcctgaat gtgacgcccg actcgttctc cgatggcggg cggtacaccg accccgatcg
     5521 cgcctttgcg cgggccgtcg agattgagga acaaggggcg gatatcatcg acatcggcgc
     5581 cgagtcgacc cgtcccgggt ccgcacggat ttcggaatcg gaagaaatgg gccggcttgt
     5641 gccggttttg aagcggcttc gcgacaaact gggaattccc atctccgtgg atacgtataa
     5701 ggccgcggta gccgaaaggg cgctggagct tggggcggag atcatcaacg acccgaccgg
     5761 gctcaccttc gatccacacc tggcgaaggt ggtcatgaat cgtgatgcag ggctgatcct
     5821 gaaccatatg cgaggcacgc ccgatacctg ggagaaactg gctccattga aagacgtaat
     5881 ggggaccatt gcgatggaga tggaagccac ggtaagccgc gccaggctgg ctggaatgga
     5941 ccggacacga attgtgatcg accccggcct gggattcggc aagcgcaagg agcagaatgc
     6001 cgagatcctc gcgcaacttc atgccctggc ccgcttcgac ttgccgctgc tggtggcgcc
     6061 atccagaaaa tcgttcctgg cgcagaaaac ggaacagggc accgcctatg caaccgcggc
     6121 cgcggtgacc gccgccattt tgaatggcgc tcacctggta cgcgtacacg atgtggtgga
     6181 aatggccgtg gtcgcgcgtg tggcggacgc ggtcctggaa tactcgggtt aatgcttctg
     6241 gcagtccgga cagaagtgag tgccccgctg cgcgatcacg atgcgccgga tgggtgtgcc
     6301 gcagcgtggg catggttgac cgggcttgcc atacacctgg tggagaagct ggaagctccc
     6361 ctggcggccc gacgcatcca cgtagtctga gatggacgat ccgcggtgct cgatcgccag
     6421 agataacact tccacaatga cgcgatgcag ccggaggcag cgctcccggc ctaatcgcgt
     6481 tgcccgggtg cgcggatgaa tgcctgcccg gtgcagtacc tcgtccgtgt agatgttgcc
     6541 gagtcccgcc aggaaggtct gattcagcag cagcggcttg atgtgcccac tgtgccgccg
     6601 gacttgccgt atgaactccg aggcggagat cgaaagggga tccgcaccca ggcgctcgac
     6661 acgtgccggc acgcactcgc cccattcgat acgcccgaat tggcgtatgt cgtcgaaccg
     6721 gagacttccc gaatcgagtg cgaagaccgc gcgtgtataa ggacctttac ccgaatccag
     6781 cagcagcttg cccgtcatcc ccagatgaaa ggtgagaaaa ccccggtcca ggcggcagac
     6841 gatgaacttg ccttgccgta tcaggctgac catgcggcgg ccgcgtagaa agtcgatgcc
     6901 ggcctgggcg gaatccaaca aaatcaaacg cgaaagaaat tcgacgtcac gaatgagacg
     6961 gcctgccagg gcgggcgcga gctcacggac gatggtttcg atttccggca gttcaggcac
     7021 tcagggcagg tccacaaaaa tgatatcgtc ctcgatcgcg acggagtgct tcttcaaccg
     7081 gatggctgga tcgaaatcgt attctccgct gcggcagtca aactcccagg cgtgccacgg
     7141 acagacgatg gcagccccgt gcagagcgcc atgagcgagc agcccgccgc gatgcgggca
     7201 gataccgtcc acggcgtgga tttcgccatc gatgttacag acggcgtaca cgccgcagtc
     7261 tgtatccacc tgtagcatcg aaccggatgg aagcgcggcg gccgggccca ccctcacgag
     7321 agccataaga gatacgctag cacggacccg tcgtgtcacg aatagacatt ccgtgctact
     7381 gtaaaatcaa tccgatgcac ataccggacg gattcctgtc gactcccgtg tggctcacga
     7441 tgaatgccat cagcgtgccc gcggtagggg tgctggcgcg ccgcgcgcac agccatctgg
     7501 acgattcgcg gattccgctg ctcggggtga tgggcgcttt tgttttcgcg gcgcagatga
     7561 tcaatttccc cgtcggcgcg ggcaccagcg ggcacctggt gggtggagcg ctgctcgcgt
     7621 gcactctggg tccggctgcc gccacggtag tgatgacggc gattctggcc gtgcaggcac
     7681 tggtgtttca ggacggaggc gtgctgagtt tgggcgccaa tgttttcaat atggcggtgt
     7741 tgggggtcct ggcgggctat ctgccctatc atttctgggg cgcgggccgc ctccgcaagt
     7801 tctcattctt cgccggcggc gtcctctcgg tgctggtcag tgccgctttg acggtatctc
     7861 aacttctgat ttcgggcatt ccgatgcgga cgggtgttct ggcgatttcg gcgggtctgt
     7921 ttctgatcag tgcgctgatt gaaggcctca tcacggtagc ggtgatcggc gcgctggaaa
     7981 aactgaatcc caactggatt cacaagccga tgtccgaagg ccggctgggt atgggcttgc
     8041 tcgcatcggc cgccgtactg ttggcggtgg tgggtgttgt ggttgcgtcc gccgctcccg
     8101 atggcctgga gagtctcgcc gaacagattg gactggcgga caaagccacc gttcttttct
     8161 cgacgccact ggcggattac gaagccaact ttctgaattc ggattggttt cgcaaagccg
     8221 cggccggaat ggcaggattg atgttgatct tcggcgtctg catcctatgc ggacgcatga
     8281 tttccaggcg taggagcgcg taaaattgca tcacgtggtg ctcgaccgct ggagcagacg
     8341 caccagcccg ctgcaccggc gcgatgcacg agcaaaaatc ctggcggcac ttgcactgct
     8401 gatctccata gcgacgacag acgcgggcat gcttccgatg ctggctgcct atggcgccat
     8461 cgtggctgcg ggttttctgg cggcccggct gccgctattc caagccgccg ctcgcgccgg
     8521 tgtggtgctt cccttttccc ttgcgatggc gctggtcagt tgggtttcag gagacccggc
     8581 gcgcgccgcg agcgtggtag tgaagagcta tctctcggct tcgatagtgc tgctgctgat
     8641 tgccaccacg ccgctgaccg aactcctctc gggcgccgaa aaactcggtg ctccaagagt
     8701 ggtgacgctc gtcgtccaat tcctctatcg atacctgttc gtgatctccg aacaggcaca
     8761 acacatgctt caggccgccc gctgccggtc cggcagcagg aacaggggga ctcgcaggcg
     8821 tttccaagcg gcggccggcg cccttgcggt cctcttctcg aaatcctata tacgggccga
     8881 aggcattcac aacgcgatgc ttgccagagg ctttcagggc aggctgccgg ccattcgggt
     8941 tcaacggttc gagttggccg atttcggatt ccttcttgct gctctccttg tcgctgccgg
     9001 tacggggctg gccctcgggg gcatcgcctg atgaagaccg ttatcgaggt ccggaacctg
     9061 cgatttcgct actccgacgg caccgtggct ctggatggtg tcgattttga cttgtattcc
     9121 ggggaaacgg tggcgttatt aggggcgaat ggatccggaa aaacaacctt tatccttcac
     9181 ctgaacggac tcctttccgg cgaggggtcc gttcgcgtct gcggcattcc cgtggaaaag
     9241 aagactctgc ctctaatccg ccagaagatc ggcctgctgt ttcaggactc tgacgagcag
     9301 ttatttatgc cgacggtgtt ggaagatgtc gcgttcggtc cgatgaatgc cggcctcagc
     9361 cgggacgaag caattacaaa ggccgggaaa gctctccacc aaaccggcat ggcgcatgtc
     9421 tccgacaagg cgccttatca tctcagtgcg ggtgaaaaga agagagtggc gctagcgggc
     9481 gtacttgcaa tggagccgga gatactcgtt ctggacgagc cgaccaccta tctcgacccg
     9541 ccgggccagc gcgagttact ccggttgata acgggcttac cgcagccgaa gatagtggtt
     9601 actcacgata ttggtttcgc ctcccggatg ggtgcccgag cggttttttt tgagaaaggc
     9661 cggatagtcg agaccggcac gcttcgagac atcgtccggc gcttccactg ggaagtaagt
     9721 tcagactgag cgcagggcgc cgctcgcctc acgccgcctc ggaggcgctt tttacagcac
     9781 tgacggccct gcgatccggc cgcacacgtg gctttctcga agagttcgac gctgcgttct
     9841 tgcggtattc cgcccaccgt ttcttttgcg cggccgcgat cctgcgtctc gcagccggac
     9901 taagtttgcg cttctgcccc gtactcacgg ggaccgacga tacgctgctc ttgacacccg
     9961 ggcgtttgcc taacaacgag cgcacatgcg tgatttgttc atcgatgcgc ttgcgctgta
    10021 attcaagtcc ttccagagcg gcttcgagga gggatatgtc gttcacggaa gactttcttg
    10081 ccatgtaagc tcccattaaa accataacat actattcatt tattgtttcc atgagattca
    10141 caaataatag tcaccggtcc ttcgtttgta aggcgcaagc tcatgaaagc ctgaaaaatt
    10201 ccggtttcaa cccggattcc gctggcgcgc gcggcactta caaagtattc gtaaagttct
    10261 ttagcctgct gaggatcggc cgcgcggtcg aaactgggac gccgcccctt ccggcagtcc
    10321 cccagaagag taaactgcga gaccaccagc agactgccgc cagcctgctg gatatttttg
    10381 ttcattttcc ccgtttcatc tggaaaaatc cggagagaaa gcgccttttc tacaaggtaa
    10441 tccgcatcct tccgggtgtc tccttttgcg actccgagca gcacaacaag tccggattcg
    10501 atagacccga cggtgtcggc ccccacttga acatgagagc tgccggcgcg ctgaagcacc
    10561 atgatcatgt aagccgccac ggcatgctat aagtatgcca gaggccaact ggaatcacgc
    10621 atgatcgagt gctacgccta cgcgcgcgcg ggactattgg gcaacccgag cgatggatac
    10681 ttcggaaaga cgatctcttt tcttgttcgc aacttccggg caagggtact gttatacccg
    10741 agcgcccggc tcgagatacg gccctccaag gccgacatgc ccgtatttga gaacctggag
    10801 gacctctata agaccacccg ctggagaggt tactacgggg ggatccggat catccaggca
    10861 ttactggtca gattcctcga ttactgcgcg gaacagggca tcgagatcga agaccggaat
    10921 ttcactctcg agtatgagac gaacatcccg ctgcgccttg gaatgggagg atccagttcc
    10981 atcattacag cggcccttcg ggcgattatg cagtattacg ggattgctat ccccctgccc
    11041 gtgcaggcca agctgatact ggagacggaa accagggaat taggggttcc cgccggattg
    11101 caggaccgcg tcattcaagt gtacgagggc cttgtatata tggacttttc gcgcgaactc
    11161 atggagcggc agggccacgg caattacgaa aagctggatc ccgcctgtct cccggatatg
    11221 tatgtggcct atcgcacttc cttgagtgag ggaaccgaag ttttccacaa cgatgtgcgg
    11281 gagcgttggc ggcggggcga ccccgaagta accggcgcga tgcaggaatg ggcctccatc
    11341 gccgagcgag ggaagacggc gattctcaat cgcgactacg gcctgttgga tgagttaatc
    11401 aaccggaact tcgatctgcg ggccacgttg taccgcatta gcccgggaaa cctggaaatg
    11461 gtcgaaacag ccaggcgtgc gggcgcctcc gcgaattttg ccggctccgg cggcgccatc
    11521 gtcggcagct accggggaga agacatgttc cgccaactca ccgccgcgat gcagagcatc
    11581 ggcgtagccg taatcaagcc acggatagac gccgggcgta ccaatcacat cgatctcaga
    11641 agctgcgcgg attcggcccg ttgatctgaa gccgatcagg cggcgacgga gcggccgctc
    11701 atgttttcga taatggcgtc cgcaaactcg ctggtcttca ccttcaccgc gccttccatc
    11761 aggcggtgca ggtcgtaggt tacacgcttc tcgcgaatgg tgccggtgat tcctttttcg
    11821 atcaggtccg aagcttcgcg ccagcccatg aagcggaaca tcatggaacc ggaaagaatc
    11881 aggctggacg ggttgatgac atcctgatcc gcatacttgg gcgccgttcc atgcgtggcc
    11941 tcgaacaccg cgtgctcgtc gccgatattg ccgccgggcg ccatccccag gccgccgacc
    12001 tgcgcggcgc aagcgtccga cagatagtcg ccgttcagat tcggggtagc gatcacatcg
    12061 tattcgtccg gcctgagcag tacctgctgg aacatcgagt cggcgatccg gtctttcacc
    12121 acgatcttgc cgttcggagc cgagctgccc aggtccgcct cggtgatgca cgtatcgctg
    12181 aactcttctt tcacgagttc atagccccag tcgcgaaagg caccttcggt gaacttcatg
    12241 atgttgcctt tgtgaaccag tgtgacgctg cgccggccat tctcgatcgc gtagcggatg
    12301 gcgctccgga tcagccgttt cgatccggtg acactgatgg gcttgatccc gatcccggaa
    12361 tctgtccgga tcttcgtgcc catctgggta ttcagaaatt cgataacccg gtgcgcttcg
    12421 cccgtacctt cgcgccactc gatacccgcg tagacatctt ccgtgttctc gcgaaagatg
    12481 acgacatcca gcttttccgg actcttcacc ggggaaggaa cacccggata gtaccggacc
    12541 ggccgcatgc agacatacaa gttcatcaat tgacgcaatg cgacattgat gctgcggatt
    12601 cctcctccga caggcgtggt caacgggccc tttatggcga cgtggaactg ctggatcgca
    12661 tccagggtgt cctgcggaag ccagttgttg aactggttga acgccttctc gcccgcgaag
    12721 acctcatacc agtaaacgcg gcgcttgcct gaataggccc gctcgaccgc ggcatcgaac
    12781 accttccgtg acgcgcgcca gatatcccgg ccggtgccgt cgccttcgac gaaaggaata
    12841 acgggattat ccggaatgac gagcttttca tcttgaacct gtatcggcgc tccgccatcg
    12901 ggaacgggaa tgttgttata ggatcgcatt gcttcttacg ggctccagtc gaaaaggaca
    12961 aacgatgata caacgtccgc cactatacca tgccaaccga gcctgccgcc atggacggcc
    13021 tggtttgaca cccctttcaa ccgcttgcta gattgggact tccatgaacc cggcagtatc
    13081 tttgaagact actcctctca atgagattca ccgcaagctc ggagcgaaga tggtcgattt
    13141 cggcggctgg gacatgccgg ttcagtacac ctctatcctc gaagagcatc acgccgtgcg
    13201 gcgggcggtg ggactgtttg acgtcagcca catgggcgag attgaggtcc gcgggccgga
    13261 agcgttgaac ctggtggagt atgtgaccac gaattcggcc tcgcggctgg agatcggaca
    13321 ggcgcagtat tcgggtttgc tgtaccgcca cggcggcttt gtcgacgacg tgctggtcca
    13381 caaggtggac gacgaccatt actttctgtg cgtgaacgcg tccaatcagg agaaagacca
    13441 cgagcacatt tcgatcgcca atagctggaa aacgaacgtg gaattcgcaa gcgaccggta
    13501 cgcgcagatg gcgattcagg gccccaaggc cctggcgacg cttcagaagc tgaccccggt
    13561 caaactcgac ggcatccgca actaccactt caccgatggg gaagtctccg gcgtgccggc
    13621 gaggattgcc cgcaccggct ataccggtga ggatggattc gagatttacg tggcaccgga
    13681 tgaagcgccg gcgatctggg ccgaactgct ggtggccggg gaagaattcg ggatacggcc
    13741 ctgcgggctc ggggcgcgca acactctgcg gctggaggcc aaaatggcgc tctatggtca
    13801 tgagattcac gcttccatca ctccttatga agcggatctc ggctggatcg tgaagctcga
    13861 caagccggat ttcatcggca agcaggcgct cgaaaagcag aaggcggaag ggattcgccg
    13921 taagctggtg ggcttcgaga tgcgcggccg gggaatcgga cgagacggat atgaagtact
    13981 gatagacggc ttgcccgccg gatgggtcac cagcggcggc ccatccccaa ccctgaacaa
    14041 aaacatcgga ctgtgttatc ttcccgcgga gttggccgcg cccggccgga cgattcacat
    14101 cctgattcgc aatcaaccgg tggaggcgat cacggtcgca acacctttct ataagcgagc
    14161 gaaatagtcc atgtacccag aaaacctgcg atatacgaaa gagcatgagt gggtcctcgt
    14221 cgagggggac actgcgacgg ttggcattac cg
//
LOCUS       DASTMK010000110         5755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_101989, whole genome shotgun sequence.
ACCESSION   DASTMK010000110 DASTMK010000000
VERSION     DASTMK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101989"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFQ79_04470"
     CDS             complement(<1..316)
                     /locus_tag="VFQ79_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138937.1"
                     /translation="MSLILHDAFGGGTMHALSGIALGQGIASARLAVLRRMVGAAVGQ
                     SATIGALARAHALAGSASGQSTAIGALRIARALAGIAANEATLSARLGVVRSLAGAPA
                     GLA"
     gene            complement(405..848)
                     /locus_tag="VFQ79_04475"
     CDS             complement(405..848)
                     /locus_tag="VFQ79_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138938.1"
                     /translation="MSVRRGAALTVSFAAVDVELRPQRKGGTPWSGGDVLVSKDGSPF
                     ANAANLPSEIGSTGRYALTLTAAEMDAAWLHVVVERDGIDPVDLLIGTAGNPSGTVFA
                     DAGNTASQFKTDRTEGTTDHWKDCLLLFTSGALAGQVKRSPASTD"
     gene            complement(838..1398)
                     /locus_tag="VFQ79_04480"
     CDS             complement(838..1398)
                     /locus_tag="VFQ79_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138939.1"
                     /translation="MTTLALLRRGSSRCRNPALPLEYSPLTIINGSGVDLRILRVGLQ
                     EVTDTDYPFWTLTFIDGLEGGEPGDVEKLDSANRDLPPGVLVRRNATVLRPGAKRGAS
                     AGGINIREILHSNVGLWTTTNWIVNDKFYNFGKYASFSGRLALREGQGIALVQRNQSG
                     LGLFEVYLRFSLSSARDAQGGALYVS"
     gene            1956..2032
                     /locus_tag="VFQ79_04485"
     tRNA            1956..2032
                     /locus_tag="VFQ79_04485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1990..1992,aa:Pro,seq:tgg)
     gene            complement(2127..2690)
                     /locus_tag="VFQ79_04490"
     CDS             complement(2127..2690)
                     /locus_tag="VFQ79_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0138940.1"
                     /translation="MGWSEQQKIEFAAPEYLKNLIQIVTETGLRIYKELAPLRKEHLD
                     LMNRTVWIPDSKTVNGVAEVPLTDIAVEAFRRQLAISGSGPFLFPSEEDPEKHQKTFK
                     TTWRATLRRAGVPYFRIYDLRSTYATRLSAGGVADEFVTQLLRQGDAKVFKKYSQMKL
                     QMKREALGKLNRNANEGGKGFDTGRPN"
     gene            complement(3248..3517)
                     /locus_tag="VFQ79_04495"
     CDS             complement(3248..3517)
                     /locus_tag="VFQ79_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0138941.1"
                     /translation="MNAIHSSIATTGSAAAAGTEVRAEERPLTVRQAAHYLGVSAQTV
                     YLWVERKQIPHLRVMGRNIRFLKSDLEPFRATFKQEMGYGTTDKV"
     gene            complement(3545..3739)
                     /locus_tag="VFQ79_04500"
     CDS             complement(3545..3739)
                     /locus_tag="VFQ79_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0138942.1"
                     /translation="MENTLDSLTVKEVAGILRCSKTHALNVIEGRVRGLPKLPHLTFG
                     RRKVVRKEWLAAWMEENKTR"
     gene            complement(4247..5608)
                     /locus_tag="VFQ79_04505"
                     /pseudo
     CDS             complement(4247..5608)
                     /locus_tag="VFQ79_04505"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
BASE COUNT         1050 a         1836 c         1686 g         1183 t
ORIGIN      
        1 tggccaaccc tgccggcgct cctgcgaggc tgcggaccac tcccaggcgg gcggacaagg
       61 tagcttcgtt ggcggcgatc ccggccaacg cccgcgcgat acgaagcgcg cctatggcgg
      121 tactttgtcc ggatgcactg ccggccaacg catgggcgcg cgcgagggcg ccgatggtcg
      181 ccgactggcc gaccgctgcg ccaaccatgc gccggagcac agcaagacgc gcactggcga
      241 tgccttggcc gagggcgatc ccggagagcg catgcatcgt cccgccgccg aacgcgtcgt
      301 gaaggatgag cgacatggag tgcgtggtta caggttgatc agaacgaaac ggtcgccagc
      361 attcggcgcg ccggtgaacg ccgagctaac cgctgcaaac ttggtcaatc cgtcgaagcc
      421 ggagacctct tcacctgccc ggcgagtgcg ccgctcgtga acaagaggag gcagtccttc
      481 cagtggtccg tcgtgccttc agtgcgatcc gtcttgaact ggctcgccgt gttcccggca
      541 tcggcgaaca ccgttcctga tggattgcct gccgtgccga tcaataggtc cacgggatcg
      601 atgccgtcgc gctccacgac tacatgcagc cacgccgcat ccatttcagc agcggtgagt
      661 gtcagggcgt accgtccggt gctgccgatc tcgctgggca ggttggccgc gttcgcgaac
      721 ggactaccgt ctttgcttac gagcacatca ccgcccgacc agggagtccc gcctttgcgc
      781 tgcggtcgta gctccacgtc cacagccgcg aaggacaccg tcagtgcggc gccgcgccta
      841 actgacatac agcgcacctc cctgcgcgtc gcgcgccgag gacaacgaga accggaggta
      901 cacctcgaac aggccgaggc ccgactggtt cctctgaacg agcgcgatcc cctgtccctc
      961 ccgcagcgcc agccgcccgc tgaatgaggc gtacttgccg aagttgtaga acttgtcgtt
     1021 gacgatccag ttcgtggtcg tccaaaggcc aacgtttgaa tgcaagatct cgcgtatgtt
     1081 gatgccgccc gccgaagcgc cccgcttcgc tcccggccgt aggacagttg cgttgcggcg
     1141 aacaagcaca cccggcggca ggtcccgatt cgcggagtcg agtttctcta cgtcaccagg
     1201 ctcgccgccc tcgagcccgt cgatgaacgt gagcgtccag aatggatagt ccgtgtccgt
     1261 cacctcctga aggcccacgc gaagaatccg cagatcgacg ccgctgccgt tgatgatcgt
     1321 caggggcgaa tactcgagcg gcagcgccgg attccggcag cgtgatgaac ctcttcgaag
     1381 tagcgcgaga gttgtcacac cggctcgctg gcatattcgt tagggacgct ggcggacgca
     1441 gggccgtttt cggcggagtg gataagttcc agatcgatcc ttactggcgt gattgcatac
     1501 ctttttatga gtactttcac agcgacaacg gagccggcat cggcgcgagt catcagactg
     1561 gctggacggg actggtagcc aggttcatcc agtttttcgg ccacgtcgct ggtccggaac
     1621 tgcttaacgc cgatgggacg cggcccctga cgcgcgcttt tctgcgacgt ccaccgcggg
     1681 tgactcagct gagcgcatga atcgaccaag ggtcagcccg tgatcgaggg cgatcggagg
     1741 ggcgggctgt gtaggtggcg ccagttgagt gctggcggta ggcaatggcc tgattgcctt
     1801 gtgaattctt tgaaagcagg tgcgtgaggg acgttactcg atcaagagaa tcaatggatt
     1861 gccacaattt agactagcgc aatgggccga ttgggccagt ccggacagtg tcgcaagttc
     1921 aggaagtggt ttaaaatcaa agaagcttca aatgtcgggg cgtagcgcag cctggtagcg
     1981 cacctgcctt gggcgcaggg ggtcgggcgt tcaaatcgcc ccgccccgac caaatcttca
     2041 gtgattctaa aagacttgat cttctgcctg ccatcgttcc cacccgtttc cagccctcac
     2101 tgtgtcagaa ctgtgtcaaa acccctctag tttgggcggc ctgtgtcaaa acccttcccg
     2161 ccttcgttgg cgttccggtt caacttgccc agggcctccc gcttcatttg caatttcatc
     2221 tgtgagtact tcttgaagac cttcgcgtcc ccctgccgga ggagctgcgt cacgaattca
     2281 tccgcgactc cgccagcact cagccgcgtg gcgtatgtgg atcgcaggtc gtagatccga
     2341 aagtacggaa ccccggcccg acgcaaggtg gcacgccagg ttgtcttgaa agtcttctga
     2401 tgcttttccg ggtcttcctc gctcgggaac aagaacggcc ccgagcctga aatcgccagt
     2461 tgccgccgga acgcctcaac cgcaatgtcg gttagcggca cctcggcgac tccgttgact
     2521 gtcttggagt ccggaatcca aaccgttctg ttcatcaggt cgagatgctc cttccgcagg
     2581 ggcgcgagct ctttgtaaat ccggagcccg gtctccgtga cgatctggat caggttcttg
     2641 aggtactcgg gagcggcaaa ctcgatcttc tgttgttcgg accaccccat gtaatgcggc
     2701 cgaaagagac catcgacccg cgtggggaac tcgaccccag gagcacgggt ttgccgcgat
     2761 gtacttcttc cgaaccgcta cgttcaacgc gcgacgaagt acacgatact cctggtgcac
     2821 cgtcgtcgcc ttcagtagtt ccttctgaat aacccccttc ccggttctaa cctcaacgcg
     2881 cttcttcagg cgctcgcgca gatacgcctc gatgtcgtcg gcggtgagat cacccaatcg
     2941 cgtttcttcg aatgaagctc tcaggtgccg cattgcacgt tgattgacct cgtgagtctt
     3001 cggcgcgcga aacgggggcg tggagaagtt cttcaaatag aactccgccc attccccgaa
     3061 cgtgagccct tgccctctgc gcacggcggg aagggtgttg ttgtccctgg cttgcaagcg
     3121 ttccctgaga atctgttgcg cttggtccca atcctcagtc ccggtcgact ccctctggcg
     3181 gtggccggcc ttgtcccggt actgcatcca ccagactgcc gattcaggtc gtctaaacaa
     3241 gctgccatca tactttatcg gtcgtgccat atcccatctc ctgtttgaaa gttgcgcgga
     3301 agggctcaag atccgatttc aggaaccgaa tattgcggcc catcacgcgg agatggggaa
     3361 tctgctttcg ctcaacccaa agatacactg tttgcgcgct cactccgaga tagtgagccg
     3421 cctggcgaac cgtaagcggg cgctcttccg cccggacttc cgttccagcc gccgccgcgc
     3481 tgccggtcgt cgcgattgag gaatggattg cgttcatgtt gatgactcga actcggcccg
     3541 tgcgctatcg ggtcttgttc tcctccatcc atgcggcgag ccattccttc cgcacgacct
     3601 tgcggcgacc aaacgtgaga tgcggcaatt tcggcagtcc gcgcacgcgg ccttcgatca
     3661 cgttcaaggc gtgcgtcttc gagcagcgta gaatgccggc gacctctttc accgttaggc
     3721 tgtccaatgt gttctccatg cgtccctcca gtctgttcgg cgtgaggacc gcgcagcgtg
     3781 acgccaattc tcatgggcgg ccacatttgc cgttgaagac cgtggcgggc ggagtcgcaa
     3841 agacagctac ggtggtatcc gcaaggtcac ccagttcggc attctagggg cgctgccttc
     3901 gtttccgtgg tcgccgcatc ggcattctag gggcgctcag cgactttgtc gatgcctgat
     3961 cgtgtcttcc gggggaacgc tgaggcggtc gaagcggcag tcggcattct agcctgcata
     4021 tctcacgagg gcggtggcga agcatctcgg ggaggttgat gagcaagctg aagggccggg
     4081 tcggcaagtt ggtgtggccg atgggcctga ttgggctccg cggctgtgat tgagcgtcgc
     4141 ggacccctcg gaattggatg ccgatcacgg tctgtgggcg cactttggct gagccggaaa
     4201 atgcaaaacg gcgaagtgat tcgttctcaa cgggcgattt tgtgagctag acccgccaag
     4261 cggcaaacgc ggcctcaaac tgactccgcc atgcatacgc gctgctgtag ctcacccaca
     4321 cacgacgcgc cgtcaccgtg atccgcgcgg cgaccttcag cagccgtagc cggatggttc
     4381 cggcttgggc ctcggcccat tcggtccggc gcagcgccag tcgccgcagt gcgtgtacca
     4441 gcacgtaggc cagcgacgac aagtacagac gcagttggtt ggagcgcatg gtctccgcgc
     4501 tcagccggtc cgcaaacagg ctcatctgtt ctttgatgcg gttctccatc tcgccccgct
     4561 cgcagtacag cttctcctac agcagccgcg ctggccacgc cacagcagcc agcgatgtca
     4621 ccacgtagcg cggattctcc tggcccgcga tctgttcggc cttggccact atccggcgcg
     4681 cccggctcca actctcccgc gtctggtaat caaattcagt aaacacgcgc gcggctttgc
     4741 tcgtgcgctc gtgctcagtc ctggcctgct gcatctgcgg ttcgatcagc gcccgcagcc
     4801 gctcattgcg tgcgaagcca aaaacataat cgactccatt cgcttcgcac caactcatca
     4861 actcgtcgcg gcacaagccc gagtcggccc gcaggatgat ttgcgtcttc ggccagcgtg
     4921 cacggatctg cgccgccacc gcggccactt cctccagagc ccccgcgctg ccgtcgatgt
     4981 gggctggacg cagccgcgcc cgcagcagat gatcgccgca gaaaatgtac agcggcaggt
     5041 agcagtagtg gttatagtag ccgtgaaaga agcgctgctc ctgccggccg tgcagcgcca
     5101 cgtcggttga gtccagatcg agcacgatgc ggcgtggcgc ccgggcgcgc gactccagaa
     5161 acaccgtcac cagcaactcg tcgatggcct cggccgagta gctgatcctg gaatagcgat
     5221 ccttcagtgg atcgccctcg ggcgtccatt ccatccggtt cagcgtgctc ttgccacacc
     5281 gcagctcgcc gatcttcctc ttcccggcca gcaccgccat cagcggatcg cgccgcaact
     5341 gctcatgatc gttgacgtcc tcatagccca atgctagacc gtagatccgc tgcgccagca
     5401 tctcgccgac cgggtgctcc acacgatccg cccggcggca atccgtaaag catccagcca
     5461 gccgcttcaa aagcccgatc cgcttgtcgg cttgcctcag caacagtgcg cctccgttgc
     5521 tggtgagcgt cccgccgtca aaactcgccg tcacctgcct cgaataatgc gcttcaaact
     5581 cgaaccgttc ctggctacac tctgtcatag cgaaagccgt ctcctgtctg gtcacaactt
     5641 tgtctggaaa ctcagttatg ccccaggaaa cggctttttg ctactactct tcgtgagaaa
     5701 tccgggctta gccccaatac tgttcactta atgattttga tagatgcgga gaagt
//
LOCUS       DASTMK010000111         6271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_198056, whole genome shotgun sequence.
ACCESSION   DASTMK010000111 DASTMK010000000
VERSION     DASTMK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198056"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VFQ79_04510"
     CDS             <1..1069
                     /locus_tag="VFQ79_04510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU0138943.1"
                     /translation="LFTKLGYVVALLTGSSTQREKTQIKRLVAAGMVHVLIGTHAVIE
                     ADVEFHKLGLAVIDEQHRFGVVQRLRLVQKGVHPDVLVMTATPIPRTLALTIYGDLDT
                     SVIDELPPGRKPVVTKHVSEDRIEQVYSFLKKQVEAGRQAYVVYPVIEESETRAMKAA
                     QKMHKHLSEIVFPGITVGLLHGRLSSEEKEAVMDLFKRGEVKILVTTTVIEVGVDVPN
                     ATVMVIEHAERFGLAQLHQLRGRVGRGSTQSYCILVTGKLNESGRERIRTMVDSTDGF
                     HIAEMDLKLRGPGEFFGTKQSGLPVLRFGNILRDQEVLEVAQREAASFVEHPPTEEEL
                     KHAVQYIRDHWQRRYGLVQVG"
     gene            1070..1603
                     /gene="rsmD"
                     /locus_tag="VFQ79_04515"
     CDS             1070..1603
                     /gene="rsmD"
                     /locus_tag="VFQ79_04515"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU0138944.1"
                     /translation="MRVIGGEFRSRRIRSLSGMDVRPTPDRLRETLFDVLAPRIEGVV
                     FVDAYAGTGSVGIEALSRGAKHVIFIERTRSSAEIIRANLASLGIERRARVVEGKVAS
                     RLKSVRGDIVFADPPYRLEPEYGTVLSILGASPPPLAILQHASRFQLKDEYPGLRRQR
                     IIQQGDNSLSFYEPASG"
     gene            1645..2898
                     /locus_tag="VFQ79_04520"
     CDS             1645..2898
                     /locus_tag="VFQ79_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0138945.1"
                     /translation="MSTSSALPADTGTPEPQNAGAQRIIDLEAQYLLQNYKRAPIVIH
                     KGKGCYVYDTAGTRYLDLVSGIGVMALGHAHPRILRVIKEQSALLIHCSNLYYNEHQG
                     PLAERLAKISGLERSFFCNSGTEAMEGALKMVRAHGRKVSPEKYEILSLDNSFHGRTL
                     GALSATGQPKYRTDFEPLLPGVRFIPPNDLAVLEAAVSDQTAGILIEGIQGEGGVYRL
                     SAEYLRKARELADRYNALLVLDEIQCGVGRTGSYFSFQTADAGIQPDVLTAAKPLACG
                     LPLGVIVTNEKAASAIGPGMHGSTFGGGALACRVALEFLDILEELLPSIMEVGGYFRD
                     RLETLAKKHSFIKEVRGYGLMIGVELDIPGKDMVQQAMAQGMLINCTQDVVLRFLPPY
                     IVTKKEIDKAVKILERVFKSAKSAQ"
     gene            complement(3003..4082)
                     /locus_tag="VFQ79_04525"
     CDS             complement(3003..4082)
                     /locus_tag="VFQ79_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0138946.1"
                     /translation="MPRATYYGLIMAGGRGTRFWPQSRKTRSKQVLRFFGDQSLIHQT
                     VERLRPVLPPEHIWIITNEFLRDEIVRQLPQVPRKQIIAEPAQRGTAPCIGVAAHILQ
                     SVDPNAVMGVFPADHLILKPARYMRLLRRAFRSAAEGRMTVLGIQPKWVETGYGYIEF
                     PKNVEAGSLELIPVRSFKEKPKEAVARRYVKAGHYFWNAGMFFWRASVYLEALRIHQP
                     KTATVLAALPRFTDKAFSKVFKDSYALCDAESVDRGVLEKAANIVGLAADDIGWSDVG
                     SWNSVYQLADPVKDGNVARSEAVFEDSTGNFVDAGRKFVALLGIRNVVVVDTPDALLI
                     ADRDRSQDVGKIVKRLEAMKRDDLL"
     gene            complement(4126..4947)
                     /gene="lepB"
                     /locus_tag="VFQ79_04530"
     CDS             complement(4126..4947)
                     /gene="lepB"
                     /locus_tag="VFQ79_04530"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0138947.1"
                     /translation="MPGKNMEKEKSAVAEQTPEPPKGDKDPPRGTIAEWTVTILLLLF
                     GTTTLVQAFVIPTGSMEDTLLIGDHLLVDKLAYAPPGPISRYILPYQEVERGDIIVFR
                     YPVDIRQTFVKRVIGVPGDRIRIENKQVYLNGTKIVEPYKYHKMEYIDSYRDNFPGDP
                     NTRLSDSGMEMLAEHVRDGEVIVPPDHYFAMGDNRDSSLDSRYWGFVPRINIIGKPLI
                     IYWSYDAPTERLAAPGIGLDHLLDLAQNFFTKTRWRRTFMLIHGYDWSEAPQTTQ"
     gene            complement(5004..5312)
                     /locus_tag="VFQ79_04535"
     CDS             complement(5004..5312)
                     /locus_tag="VFQ79_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138948.1"
                     /translation="MPLDEDMFAMAEPAQRQQPKHHWRQKVIGLLVTILCFEIGFFLL
                     VFPWTELWSQNYFAHFGPRWNLVWNSPYFRGAVSGIGIIDLYISFLEILRLRRFSGGV
                     "
     gene            complement(5303..5992)
                     /locus_tag="VFQ79_04540"
     CDS             complement(5303..5992)
                     /locus_tag="VFQ79_04540"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU0138949.1"
                     /translation="MPAFRCVGTYERQARRDGYCLVAGIDEAGRGSLFGPVFAAAVIL
                     PEDHRIRGLRDSKLVQLEEREELEDSIRQRAICWSVSAADVFEIDRYNIYHASRLAMK
                     RAVQNLLPAANFLLVDAMKIDLPIPQRALIHGDARCQAIAAASILAKTHRDRCLEEWD
                     RVFPEDGLRSHKGYSTPEHRRALRAYGPTTLHRFTFDPVRSTSPPEMLPPGAAEQTLL
                     FPAEAEEAACL"
     gene            complement(6008..>6271)
                     /gene="rplS"
                     /locus_tag="VFQ79_04545"
     CDS             complement(6008..>6271)
                     /gene="rplS"
                     /locus_tag="VFQ79_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU0138950.1"
                     /translation="VHVKIREADKERTQAFEGTVIARKNSGLGETVTVRKISFGHGVE
                     RIFPLHAPVIDHIDVIRTGRVRRSKLYYLRALRGKAARLKERE"
BASE COUNT         1306 a         1819 c         1839 g         1307 t
ORIGIN      
        1 cctgttcaca aaactcggct atgtcgtggc gctgttgaca ggctcgtcca ctcaacgcga
       61 gaaaacacag atcaaaagac tggtggccgc cggaatggtg catgttctca taggcaccca
      121 cgcggtgata gaagccgatg tcgaattcca caagttgggc ttggcggtca tcgacgaaca
      181 gcaccggttc ggtgtcgtac agcggctccg cctggtgcag aaaggggttc acccggacgt
      241 gctcgtgatg acggccaccc ccattccgcg cacactcgcc cttaccatct acggggatct
      301 ggatacttcc gtgatcgacg agctgcctcc gggacgcaag cctgttgtca cgaagcatgt
      361 ctcggaggac cgcatcgagc aggtgtacag tttcctcaaa aagcaggtgg aagcgggcag
      421 acaggcttac gtggtttacc ccgtgatcga agaatcggaa acgcgggcaa tgaaagcggc
      481 gcagaagatg cacaagcatc tctcggaaat cgtcttcccc ggcattaccg ttggactgct
      541 gcacggccgc ttaagctcgg aagaaaagga agcggtgatg gacctcttca agcgaggtga
      601 agtgaagatc ctcgtgacaa ccaccgtgat cgaggtgggc gtggacgtgc cgaacgcaac
      661 ggtcatggtg attgagcacg ccgaacgctt cggcctggcg caattgcacc agttgcgagg
      721 ccgcgtaggg cggggctcga cgcagagcta ctgcattctg gtgactggca agctcaatga
      781 aagcggccgg gagagaattc ggaccatggt ggattccacc gacggcttcc acatcgccga
      841 gatggatctg aagctgcgcg gaccggggga gttcttcggc acgaagcaat cgggcctgcc
      901 ggtactccgg tttggcaata tcctccggga tcaggaggtg ctggaggtag cacagcgaga
      961 agcggcctcc tttgttgagc atccgccaac tgaggaagaa ctcaagcatg ccgtacagta
     1021 cattcgcgac cactggcaac gacgctacgg cctggtgcag gttggttaga tgcgggtgat
     1081 tggaggtgaa tttcgcagcc gccgcatcag gagcctctcc ggtatggacg tacgcccaac
     1141 gccggaccgg ctgcgggaaa cgctgttcga cgtgctggcg cctcgaatcg agggcgtcgt
     1201 gttcgtggac gcctacgcgg gcaccggctc ggtcggcatt gaggcactga gccgcggagc
     1261 gaaacacgtc attttcatcg agcggacccg gtcgtcggcg gaaatcattc gagcaaatct
     1321 tgcatcgctc ggtatcgagc gccgggcgcg ggttgtcgaa ggcaaagtag cgtcacgcct
     1381 caagtctgtg cggggcgata tcgtttttgc ggacccgcct tatcgtctcg aacccgagta
     1441 cggcacggtg ttgtctattc tgggagcttc gccgccgccc ctcgctatcc ttcaacacgc
     1501 ttcccgcttc cagttgaaag acgagtatcc cggactgcgg cggcagcgga tcattcaaca
     1561 gggcgacaac agcctctcct tttatgaacc agcctccgga tgaagcggac agagcgcctt
     1621 tggcgcgtga agtatcatcg aaagatgagc acttcttccg cccttccggc ggacactgga
     1681 acccccgaac cgcagaacgc cggtgcacag cgaatcatcg atctcgaagc gcaatatctg
     1741 cttcagaact ataaacgtgc acccatcgtc attcacaaag gcaagggctg ctacgtgtac
     1801 gatacggcag gcactaggta tctggacctt gtctccggca tcggagtgat ggcgcttggc
     1861 catgcgcacc cgcgcattct gcgtgtcatc aaagagcaga gcgcactgct cattcactgc
     1921 tcgaatctct actacaacga gcaccaggga ccgttggctg agcggttggc gaagatcagc
     1981 gggctggaac gaagtttctt ctgcaactcg ggaacggagg cgatggaagg tgcgctgaag
     2041 atggtccggg cgcacgggcg taaggttagt ccggaaaagt atgaaattct ctcgctcgac
     2101 aattcctttc acggccgcac gctcggtgcg ctctcggcga cagggcagcc caagtatcga
     2161 acggattttg aaccgctgct ccccggcgtg cgatttattc ccccgaatga cctggccgtg
     2221 ctcgaagccg cggtcagcga tcagacggcc ggtattctga tcgaagggat ccagggcgaa
     2281 ggcggcgttt acaggctctc cgccgaatat ctgcgtaagg ctcgcgagtt ggcagaccgc
     2341 tacaatgcgc tcctggttct cgatgaaatt cagtgcgggg tcggccgcac cggctcctat
     2401 ttctctttcc agaccgcgga tgcgggaatt cagccggacg tattgactgc ggccaagccc
     2461 ctggcgtgcg gtctgcctct gggcgtgatt gtcaccaacg agaaagcggc atccgccatc
     2521 ggcccgggaa tgcacggctc tacatttgga ggcggagcgc tggcatgccg cgtcgcattg
     2581 gaattcctgg atatcctgga ggaactgctg ccctcgatca tggaagtggg tggctatttc
     2641 cgcgacaggc tcgaaacact cgcgaagaag cactccttca tcaaggaagt tcgcggctat
     2701 gggctgatga tcggcgtcga actcgatatt cccggcaagg acatggttca acaggcgatg
     2761 gcgcagggta tgctcatcaa ctgcacgcag gacgtggtgt tacgttttct gccgccctac
     2821 atcgtcacga agaaagagat cgacaaggcg gtgaagatcc tggaacgggt ttttaaaagt
     2881 gcgaaatccg ctcaataatc gcgccagttc gccaagaccg cttgctggcg cgcgcggctc
     2941 cgtaatcgct tctgagccgc gacgtaagtg agcggttggc atggttattc tgtgacagtc
     3001 ccttagagca gatcgtcgcg cttcatcgct tccaggcgtt tcacgatctt cccgacgtcc
     3061 tgtgacctgt cgcgatcggc aatcaacagc gcatcgggag tgtccaccac caccacattc
     3121 cggatgccca acagtgccac gaattttcgt cccgcatcga cgaagtttcc ggtactatct
     3181 tcgaataccg cttccgatcg agcgacatta ccatccttga cggggtccgc aagttgatag
     3241 acggagttcc aactgccaac atcgctccag ccaatgtcgt ccgccgcgag cccgacgatg
     3301 tttgcagcct tttcgagcac accgcggtca accgactcgg catcacagag cgcatagctg
     3361 tccttgaaaa cctttgaaaa cgccttgtcc gtaaaacggg gcagcgccgc cagcaccgtg
     3421 gcggtttttg gctggtgaat ccggagagcc tccaggtaca cgctggcacg ccagaagaac
     3481 atcccggcat tccagaagta atggccggct ttcacatatc gtctggcaac cgcttccttc
     3541 ggtttctcct tgaacgatcg cacgggaatt agttcgagcg atcccgcctc gacgttcttc
     3601 ggaaattcga tgtagccata gccggtctca acccatttcg gctgaatgcc tagaacggtc
     3661 atccgtcctt ccgcggcgga acgaaaagcg cggcgcagca ggcgcatata gcgcgccggt
     3721 ttcagaataa ggtggtctgc cggaaaaacg cccattacgg cattggggtc gacggactgg
     3781 agaatgtgtg cggccacgcc gatacacggc gcggtgccgc gttgtgccgg ttccgcgata
     3841 atctgttttc gcggcacctg gggcaactgg cggacaatct catcccgcag gaactcgttg
     3901 gtgataatcc aaatatgttc ggggggcaga acgggacgca gacgctccac ggtctgatgg
     3961 atcagcgatt ggtccccgaa gaaacgcaga acctgtttgg atcgtgtctt tctgctctgg
     4021 ggccagaatc gcgtgcccct tccgccggcc atgataagcc cgtagtaggt cgctcttggc
     4081 atgtagtcct ccccgccggc gcgctgcaat gaacgccggg cggagctact gcgtggtttg
     4141 cggcgcttcg ctccagtcgt agccgtgtat cagcatgaac gttcgccgcc atctggtttt
     4201 agtgaaaaaa ttctgagcca gatcgaggag atgatcgagc ccgattcccg gcgccgccag
     4261 ccgttccgtc ggcgcatcgt aggaccagta gataatcaac ggtttgccta tgatgttgat
     4321 ccggggaaca aagccccaat agcggctgtc cagggacgaa tcgcggttat ctcccatcgc
     4381 gaagtagtgg tccggtggaa cgatgacttc tccatcgcgg acgtgttccg ccagcatctc
     4441 catgccggaa tccgaaagac gggtattcgg gtcacctggg aaattatcgc ggtaggagtc
     4501 gatgtactcc atcttgtggt acttatacgg ctccacgatt ttggtgccgt tcaaatagac
     4561 ctgcttgttt tcgattcgga tccggtcgcc gggaacgccg ataacccgct tgacgaacgt
     4621 ctggcggatg tcgacagggt agcggaaaac aatgatgtcg cctctttcca cctcctgata
     4681 cggcaggata tatctgctga tggggcccgg cggtgcgtag gccagtttgt ctaccagcag
     4741 gtggtcgcca atcagaagcg tgtcctccat ggagccggtt ggaatcacga acgcctgcac
     4801 cagcgttgtc gttccaaata gcagcaggag gatggtcacc gtccactcgg cgatggtgcc
     4861 ccgaggcgga tccttgtcgc ccttaggcgg ctccggcgtc tgctccgcca ctgcactctt
     4921 ctctttttcc atgttcttac ccggcaatgg agattctaac ctttaattgt atcgaaatac
     4981 ggcttccatc attccgaact tactcaaacg ccgccggaaa agcggcgcaa tcgaaggatc
     5041 tcgagaaacg agatgtaaag atcgatgatg ccaataccgc tcactgcccc cctgaaatag
     5101 gggctgttcc agaccaggtt ccagcgcggg ccaaagtggg cgaagtagtt ttgggaccaa
     5161 agctccgtcc acggaaatac gagcagaaag aagccgatct caaaacaaag aatggtcacc
     5221 agcaggccga tcaccttttg ccgccagtga tgtttcggct gctgcctctg ggcaggttcg
     5281 gccatcgcaa acatgtcttc gtctaaaggc atgctgcctc ttcggcctct gccgggaaaa
     5341 gcagggtctg ctcggcagcg ccgggtggca acatttcggg aggactggtg gaacgcaccg
     5401 ggtcaaatgt gaaccggtgc agcgttgtag gaccatacgc gcgcagcgcc cgccggtgtt
     5461 ccggtgttga atatcctttg tggctgcgga gcccgtcttc cggaaacacg cgatcccatt
     5521 cttccaggca gcggtcgcga tgtgttttgg ccaggatgga agcagccgca atggcctggc
     5581 acctggcgtc tccgtggatc agtgcccgct gcggtatggg caggtcgatt ttcatcgcgt
     5641 ccaccagcag gaaattggcc gccggcaaaa ggttctggac agcccgtttc atcgccagcc
     5701 tggatgcgtg atagatgttg tagcgatcga tttcgaaaac gtccgcggcg gagacggacc
     5761 agcatatggc ccgctggcga atcgagtctt ccagttcctc ccgctcctcc aactgaacca
     5821 gtttgctatc ccgcaggcca cggattcgat gatcttcggg cagaatgacg gctgccgcga
     5881 acaccggtcc gaacagcgat ccgcgcccgg cttcgtcaat cccggctacc agacagtatc
     5941 cgtcccggcg agcctgacgc tcgtatgtgc cgacgcaacg gaaggctggc aacctgctgt
     6001 tttgcaacta ctcgcgctct ttcaatcggg ccgctttgcc tcgcaaggcg cgcaaataat
     6061 acaatttcga tcttctaact ctgccggttc ggatcacgtc gatgtggtcg atgacgggcg
     6121 catgcagtgg aaagatgcgc tcgacgccat gtccaaagct gatctttctg acggttaccg
     6181 tctcacccag ccccgaattc ttgcgggcaa tcaccgtgcc ctcaaaagcc tgcgtccgtt
     6241 ccttgtcggc ctcccggatc ttcacgtgta c
//
LOCUS       DASTMK010000112         8836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_70260, whole genome shotgun sequence.
ACCESSION   DASTMK010000112 DASTMK010000000
VERSION     DASTMK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70260"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFQ79_04550"
     CDS             <1..747
                     /locus_tag="VFQ79_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138951.1"
                     /translation="SGSADYKINRAVPLLFSLVAISILATVIMLALPPLLLGTRLPRI
                     EGAWSLLSYFLFLGAGYIMIQVALIQKFVLFLGHPTYALTVIIFSMLLASGLGSYFSR
                     RLVAGSDRRLEYILASITGLVIALALAATPLTSHAVGLPLPLKVIMAVLLITPAAFLM
                     GMPFPSGLARVEQRYHPSVRWAWSLNAASSVLGSASAVFLAIYLGLRDTLLVGGVMYV
                     CALLCVRLTEPARVTAAPLKAQPVPVTHRD"
     gene            complement(751..1050)
                     /locus_tag="VFQ79_04555"
     CDS             complement(751..1050)
                     /locus_tag="VFQ79_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138952.1"
                     /translation="MPVPIDFIRATLAVLAVIFAYFFGRYAVRSRQGRARQSLVLTWG
                     FRLAASLLAVFWTRQFDLIVILALIFAGASLGAGIWMELHPKQEPDLTKTMFPEE"
     gene            1135..2847
                     /locus_tag="VFQ79_04560"
     CDS             1135..2847
                     /locus_tag="VFQ79_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEU0138953.1"
                     /translation="MRYFALASDYDGTLAHHGAVEDSTIDALKRVRDSGRKLLLVTGR
                     ELPELQMIFPQLDLFDSVVVENGALLYTPSTKKEQVLADPPSERLISALRGRGIDRLS
                     VGRVIVATWEDYKSTVLEVVNELGLELQLIFNKGALMILPSGVNKASGLAAALEAMCL
                     SRHNVAGIGDAENDHAFLSLCECGVAVANALPALKERADVVMNGVAGEGVRELIDSLL
                     KDDLRSYAPRLSRYDILLGTDEAGQKVCVHPYGTSLMVAGTSGGGKTTLTTGFLERLT
                     EAKYQFCVIDPEGDYQNFTGAVVLGDTKNSPTVNEILSVLETPSEQVIVNLVGIDLDK
                     RPEFFDTLLPRLHEMRSRTGRPHWIIIDEAHHLLPASWQPSTITIPKEMTSMMLITLE
                     PDHVAPSMLACVNTLVAVGKNPEETIRKFSGAANLVTPDMQPVELKRGEVLLWCAKDG
                     RPPVRVKIQPGAAQSSRHARKYAAGELPPDRSFYFRGPEGKLNLRAQNLVMFLQLMDG
                     VDEETWLYHLRRRDYSRWFLEEIKSDDLSAATADIEGRKRLSAAESRALIREEIQRRF
                     TLPA"
     gene            complement(2858..3205)
                     /locus_tag="VFQ79_04565"
     CDS             complement(2858..3205)
                     /locus_tag="VFQ79_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0138954.1"
                     /translation="MKVHQWSQITQEQLNPLCLRQVIHCANMTLGRLRLSKGCVVPEH
                     SHPNEQFTMLLEGKLKFVFGDRIQELGPGEMLEIPPHAPHSVEALEDSVAIDLFAPRR
                     EDWLRGDDAYLRK"
     gene            complement(3255..6005)
                     /locus_tag="VFQ79_04570"
     CDS             complement(3255..6005)
                     /locus_tag="VFQ79_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEU0138955.1"
                     /translation="MKPPVVSGKPGALEPGQKIARRREHPLDVFFTPNNIAVIGATEN
                     PGTVGRTTLWNLISSPFGGTVFPVNPKRGSVLGIKAYPNIDAVPEQCDLAVIITPPPS
                     IPGIIRDCGRNGVKGAIVISAGFKEIGAPGVELERQVLEEAQKADIRIIGPNCLGLMS
                     PPTGVNATFAAGMARPGNVGFISQSGALCTAVLDWSFQEMVGFSAFISIGSMLDVGWG
                     DLIDYLGNDPRTRSILIYMESIGDARSFLSAAREVALRKPIIVIKAGRTSAGGKAAAS
                     HTGALTGSDDVLDAAFRRSGVLRVNNIADLFYMAEVLAKQPRPKGPRLTILTNAGGPA
                     VLATDALITSDGELGDLNTDTIEELNKFLPPTWSKGNPIDIIGDAAPERYAKALEVAA
                     KDPHSDGLLVVLTPQAMTDATQTAEHLKPYAKIGDKPVLASWMGGPEVAAGEKILNAA
                     GIPTFPYPDTAARAFVYMWRYTYNLRGLYETPIFAEGDGIDRKKAEDIIAKVRQEKRT
                     ILTEFESKQVLASYGIPVVETRIARTEEEAAKAAAEIGFPVVLKLFSETITHKTDVGG
                     VQLNLRDEAAVRRAYQSISSAVAEKAGAEHFLGVTVQPMIKLDGYEIILGSSLDPQFG
                     PVLLFGLGGQLVEVFKDRALALPPLNTTLARRMMEQTKIYKALKGVRGRKPVDLAALE
                     QLMVRFSQLVVEQRWIKEIDINPMLASPERLVALDARVVVHDAELTEDKLPKLSIRPY
                     PTQYVSKFTMKNGTEVEIRPIRPEDEPAMVQFHETLSDRTVYLRYLQLLKLSQRVAHE
                     RLTRICFIDYDREMALVVERKNPATGNPEILGVGRLQKAHGTNEAEFAAVVSDSHQGQ
                     GLGKELVRRLLLVARGEGIRRVRADILGDNTTMQRICESVGFKLHRDLSDPTIEAEAL
                     IE"
     gene            6156..7076
                     /locus_tag="VFQ79_04575"
     CDS             6156..7076
                     /locus_tag="VFQ79_04575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0138956.1"
                     /translation="MRRRNFLSSSLAGGLMFNAAAATSIPKRAYRDGVNLSVIGFGGI
                     VVVGHDQSDANRIVAESVDRGINYFDVAPTYGDGEAEQKLGPALEPFRKNAFLACKTT
                     ERTADGARRELEQSLKRLRTDHFDLYQFHAVTTQEDVDKILAPGGAAELFVKARKEGK
                     VRYLGCSAHSASAAIALMDRFPLDSILFPVNYVLYSQGSFGPQILEHAKQKGVARLAL
                     KSLAKTRWAKGEKRDYPRCWYRPVSEPELAKQALRFTLSEDLTAAIPPGDVSLYRMAL
                     DLAAGFKPLSQAERDELLASAQGLDPLFRA"
     gene            complement(7082..>8836)
                     /locus_tag="VFQ79_04580"
     CDS             complement(7082..>8836)
                     /locus_tag="VFQ79_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0138957.1"
                     /translation="VVNSNSDSISVIDTRKHAVVETISLKPFHTEVKGIAPTAIRLSS
                     DKRRLYVACGGINAVVVLDPASGRFLGAIPTAWYPNDLALSPDGKHLAVTALLGVGSG
                     WQGSPDRRFVHAYRGAVSVIPLPDEAQLANYTTAVAENNRMQPVGAVRPAARPANVRV
                     APKAIPLRSGDPSLIEHVVYVIKENRTYDQLFGDLGKGNGEPRLVMFGEPVAPNHRRM
                     AEQFVLLDNFYATGGNSADGHQWVTQANQTAYALWPGYIGRSYPFDGTDPIAYSEGGF
                     LWDLALKAGKSVRVYGEFAPRMPEPTSRERFGLLEAWKRGEDFSSRWSVSSPIPPLDK
                     LLARNFPTYTNAIPDMVRAQIFLKDFAGWEKDGKMPNLTILQLPCDHTFGTTPDVSTP
                     KAMVADNDLALGQIVEALSKSRFWSKMAILVVEDDAQNGVDHVDGHRTVALAVSPYVR
                     RGHVDSTFYSHQSMLKTIELILGLPTMSLFDLIANDMRASFQDTPNLEPYSAVEPQQS
                     LFELNPPAKALRGKAREGALASARMRWDVPDAAPTETLNRILWHSIKGWDVPYPAPKT
                     ALFAPLSLDIDDEDREMR"
BASE COUNT         1734 a         2643 c         2621 g         1838 t
ORIGIN      
        1 tcaggcagcg ccgattacaa aatcaacagg gctgtaccgc tgctgtttag cctggtagcg
       61 atcagtattc tggccaccgt cattatgctc gcgctgcctc ccctgttgct gggaacgaga
      121 ctgccccgga tagaaggtgc ctggtcgctc ttgtcttatt tcctctttct cggcgcgggc
      181 tacatcatga tccaggtggc tttgattcag aaattcgtgc tcttccttgg ccatcccaca
      241 tacgccctca cggtgatcat tttctccatg ctgctggcca gcgggcttgg aagttacttt
      301 agccggcgcc tggtcgcggg ttcggaccgg cgtctcgaat acatcctggc aagcatcacc
      361 ggccttgtca tcgcgctggc attggccgca acaccattga cgagccatgc tgtcggtttg
      421 ccgctgccgc tgaaagtgat tatggcggta ctgctgatta cccccgcggc tttcctcatg
      481 ggaatgccgt ttccatcggg gcttgcacgg gtggaacaac ggtatcatcc ttccgttcgc
      541 tgggcttggt ccttaaacgc ggcttccagt gtgctcgggt ccgccagcgc cgtattcctg
      601 gctatttatc tcggtctgcg ggatacgcta ctggtgggcg gagtcatgta cgtctgtgca
      661 ttgctttgcg tccgcctgac ggaacccgca cgagtcaccg ccgcccccct taaagcgcaa
      721 cccgtacccg tgacccatcg cgattgagaa tcactcttcc gggaacatgg ttttcgtgag
      781 atcgggttcc tgcttcgggt gcagctccat ccagatgccg gctccaaggc ttgctccggc
      841 gaagatcaaa gccagaatga cgatcaggtc gaactggcgc gtccagaata cggcaagaag
      901 ggatgccgcc agacgaaagc cccaggtgag tacaaggctc tggcgggccc tgccctgccg
      961 gctccttacc gcataacggc cgaagaagta ggcgaatatc accgcgagaa cggctagcgt
     1021 tgcccggatg aaatcgatcg ggaccggcat gtcttcgata ttaccaataa cgctttccgc
     1081 cggcttcgag agattgtctc tgtaacagcc actgccggca atcatctaac cagcgtgcgc
     1141 tacttcgctc ttgcctccga ctacgatggt acgctcgctc atcatggcgc cgtagaggat
     1201 agcaccatcg atgcgctgaa gcgggtacgg gattccggcc ggaaactgct gctggtgacc
     1261 ggccgcgagc ttcccgagtt gcagatgatc tttccgcaac tcgacctgtt tgacagcgtt
     1321 gtcgtggaaa atggcgctct tctatacact ccttccacga agaaggagca ggtgctggcc
     1381 gacccgcctt cagaaaggct gatctccgct ttgcgcggac gcggcattga ccggctgtcc
     1441 gtgggcagag tgatcgttgc cacctgggaa gactacaaaa gcacggtgct cgaggtcgtg
     1501 aacgagctcg gcctggaact gcaactcatc tttaacaagg gtgcgctgat gattctgcct
     1561 tccggtgtaa acaaggcgag tggtctcgcg gcggcgctgg aagcgatgtg cctttcacgc
     1621 cacaatgtgg ccggcatcgg ggacgccgaa aatgaccatg cgtttcttag cctgtgcgaa
     1681 tgcggcgtgg cggtagccaa tgcgctgccg gcgttgaagg agcgcgccga cgtagtaatg
     1741 aacggggtgg cgggtgaagg cgtgcgggag ctgattgaca gtctcttgaa agacgacttg
     1801 cgctcttacg cgccgcggtt gtcccgctac gatatcctgc tcggcaccga tgaggccgga
     1861 caaaaggtct gcgtgcatcc ctatggaacc agcctgatgg tggccggcac atccggcggc
     1921 ggcaaaacta cccttactac gggcttcctg gagagactga cagaagcgaa ataccagttt
     1981 tgcgtcatcg atcccgaggg cgattaccag aatttcaccg gtgcggtcgt gctgggagat
     2041 accaaaaaca gccccacggt taatgaaatc ctgagtgtgc tggagacacc gtccgagcag
     2101 gtcattgtaa acctggtggg tattgatctg gacaagcggc cggaattctt cgacaccttg
     2161 ctgccgaggc tccacgaaat gcggagccgc acgggacgtc cccactggat catcatcgac
     2221 gaagcgcatc acctgctgcc cgcgtcgtgg cagccgtcga cgatcaccat cccgaaagaa
     2281 atgaccagca tgatgctgat cacgctggag cccgatcacg tcgcgccctc catgctcgcc
     2341 tgcgtgaaca cgctcgtggc ggtgggcaaa aatcccgaag aaaccatccg caagttctcc
     2401 ggggccgcga acctggtaac tccggatatg cagccggtgg aactcaaacg cggcgaagtt
     2461 cttctgtggt gtgcgaagga cgggcgtcct ccggtgcgcg tcaaaatcca gccgggagcg
     2521 gcacagagtt cgcgccacgc ccgcaagtat gcagcgggcg agttacctcc cgatagaagc
     2581 ttctacttca ggggtcccga aggcaaactc aatctgcggg cacaaaacct ggtgatgttc
     2641 cttcaactca tggacggcgt agacgaagag acatggctct atcacttacg ccgccgcgac
     2701 tattcgcgat ggttcctcga agaaattaaa agtgacgatc tctcggccgc cacggcggac
     2761 atcgaaggcc ggaagcggct ttcggcagcc gaaagccgcg cgctcatccg cgaggaaatc
     2821 cagcggcgat tcaccctgcc cgcatgaacg gcagcgatca cttcctgaga taagcatcgt
     2881 cgccgcggag ccaatcctcg cggcgtggag cgaacaggtc gatcgcgacg gaatcctcca
     2941 gcgcttctac gctgtgcggc gcatggggag gaatttcgag catctcgcca ggaccaagtt
     3001 cctgtatccg atctccgaaa acgaacttga gctttccctc cagcagcatg gtgaactgct
     3061 cattgggatg gctgtgctcg ggaacgacgc accctttact caaacgcagg cgccccagag
     3121 tcatgttggc gcagtgaatc acctgccgca agcagagagg attaagctgt tcctgggtga
     3181 tttgggacca ctggtgaact ttcatagaac tcgctatctg gctaacttac cagatagcga
     3241 gttcaaatgc tctgctactc gataagcgct tccgcctcga tcgtcgggtc gctcaggtcc
     3301 ctgtgcagtt tgaaaccaac gctctcgcaa atgcgctgca ttgtcgtgtt gtcgccgagg
     3361 atgtccgccc ggacgcggcg aatgccttca ccgcgagcta cgaggagcag ccgccgtacc
     3421 agttccttgc cgaggccctg tccctggtgc gaatcgctga caaccgcggc gaattccgct
     3481 tcattcgtgc catgggcctt ttgcaggcgt ccaacaccaa gaatttccgg gttgccggtg
     3541 gcgggattct ttcgttcgac gaccagggcc atctcgcgat cgtaatcgat gaaacagatc
     3601 cgcgtgagcc gctcgtgcgc aacacgctgg ctcagcttca gcagttgcag gtaacgcagg
     3661 tacacggtgc gatccgagag cgtctcgtgg aactgcacca tggccggttc atcttccgga
     3721 cggatgggcc ggatctccac ctccgttccg ttcttcatcg tgaacttgga aacgtactgt
     3781 gttgggtatg ggcggatgga cagtttaggc aacttgtctt ccgtcaattc cgcgtcgtga
     3841 accaccacgc gcgcgtccag cgcaaccagc cgctcgggcg aggccagcat cggattgatg
     3901 tcgatctctt taatccaccg ctgttcgaca acaagctggc tgaaacgaac catcaattgc
     3961 tcgagggcgg ccagatccac cggcttgcgg ccgcgcacac ctttcagtgc cttgtagatc
     4021 ttggtctgct ccatcatgcg gcgcgccagt gtggtattaa gcggcggtaa agccagcgcg
     4081 cggtctttga acacctcgac gagttggccg cccagaccga acagcagcac aggcccgaac
     4141 tgcgggtcca ggctgctgcc gaggatgatc tcatagccgt cgagtttgat catcggttgc
     4201 accgtcacac caaggaagtg ctccgcaccg gccttctcgg ccacagccga cgaaatcgat
     4261 tgatacgccc ggcgcactgc cgcttcgtcg cgaaggttga gttgtactcc gccgacatcc
     4321 gtcttgtggg tgatggtctc cgagaataat ttcaacacca ccggaaaacc gatctccgcc
     4381 gcggctttcg ccgcttcctc ttcggtgcgg gcgatcctgg tttcaaccac gggaattccg
     4441 taagaggcga gcacctgctt cgattcgaac tcggtcagaa tcgttctctt ttcctgccgg
     4501 accttggcga tgatgtcttc ggccttcttg cgatcgatgc cgtcgccctc cgcgaagatc
     4561 ggtgtttcat agaggccgcg caggttgtag gtatagcgcc acatgtagac gaaagcgcgc
     4621 gccgcggtat cggggtaggg gaatgtcggg attccggcgg cgttcaggat cttttcgccg
     4681 gccgctacct cggggccgcc catccaactg gccagcacgg gtttatcgcc gattttggca
     4741 tagggcttca ggtgctccgc ggtttgggtg gcatcggtca tggcttgcgg cgtgaggacc
     4801 accagcaggc cgtcgctgtg cgggtctttc gccgccacct ccagcgcctt cgcatatcgc
     4861 tccggggcgg catccccgat gatgtcgatc gggttccctt tgctccaggt gggcggcagg
     4921 aacttattca actcctcgat ggtatcggta ttaaggtcgc ccaactcgcc gtcgctggta
     4981 ataagggcat cggtcgccag aacggccggt cctcccgcat tggtgagaat cgtcagccgt
     5041 ggtcctttcg gccttggctg cttggcaaga acctccgcca tgtagaacag gtcggcgatg
     5101 ttattcacgc ggagaacgcc gcttcgccgg aacgccgcgt caagcacgtc atcgcttccg
     5161 gtgagtgcgc cggtatgcga cgccgcggcc ttgcccccgg cggatgtgcg gccggctttg
     5221 atgacgatga tcggcttacg cagcgcaacc tcgcgcgccg ccgaaaggaa ggaacgcgca
     5281 tcgccgatcg attccatgta aatcaaaatg ctgcgcgtac gcgggtcgtt tccgagataa
     5341 tcgatcaggt cgccccaacc gacatccagc atcgagccga tggagatgaa cgcgctgaag
     5401 ccaaccatct cctgaaagct ccagtcgaga acggcggtgc aaagcgctcc gctctggctg
     5461 atgaatccca cgttgcccgg ccgggccatt cccgcggcga aggttgcgtt cacgccggtc
     5521 ggagggctca tcagcccgag gcaattcgga ccgatgatgc gaatgtccgc cttctgcgcc
     5581 tcttcgagaa cctgtctttc gagttcgacg cccggagctc caatctcctt gaatccggcg
     5641 gaaatgacga tagcgccttt cacgccgttc cggccgcagt cccggataat tcccggaatg
     5701 ctcggcggcg gagtgatgat gaccgccagg tcgcactgct ccggcaccgc gtcgatgttc
     5761 ggataagctt tgatgcccag cacgcttccg cgcttggggt tcacagggaa caccgtcccg
     5821 ccgaaaggcg agctgatcag gttccacagg gttgtacgcc ccaccgtgcc cggattctcc
     5881 gtggcgccga tgacagcgat gttgtttggc gtaaaaaaga catccagggg atgctcgcgg
     5941 cggcgggcga tcttctggcc gggttcaaga gcaccaggtt ttccggatac tacgggaggc
     6001 ttcatgatga ttcctttaga gacaattaaa gagcagttta tgattttctt ctctaatact
     6061 cttctttatc atccgatttc agagaagcgg gcgcaactgc acttcgcccc attgtctcca
     6121 aaaaccggcg gtacaatggc cgcaggagtc cacctatgcg gcgacgcaac tttctaagct
     6181 ccagcctggc gggaggattg atgttcaacg cagccgctgc cacctcaatt cccaaacgag
     6241 cctatcgcga tggcgtgaat ctgtccgtca tcggattcgg cggcattgtc gtcgttggcc
     6301 acgatcagtc cgacgccaac cgcattgtcg ccgagtccgt ggatcgcggc atcaattatt
     6361 tcgatgtggc cccgacgtac ggggacggcg aggccgagca gaaattgggg cccgctctcg
     6421 aaccgttccg caagaacgcg ttcctcgcct gcaaaactac cgagcgtacg gcggatggcg
     6481 cgcgacggga actcgaacaa tcgttgaaga ggcttcggac agatcacttc gacctttatc
     6541 agttccatgc ggtcacgacg caggaagatg tagataagat tctcgctccg ggcggcgccg
     6601 cggagctatt cgtgaaagcg cggaaagagg gaaaggtccg ctatctggga tgctcagcgc
     6661 attcggcaag cgcggccatt gcattgatgg accgtttccc gttggattcg atcctgttcc
     6721 cggtgaacta cgttctctat agccagggct ctttcggccc acagatcctg gaacatgcca
     6781 agcaaaaagg tgtggcgcgg ctggctctga aatccctcgc caaaacccga tgggcgaaag
     6841 gcgagaagcg ggattatccc aggtgctggt accggccggt atcggagccc gaactggcga
     6901 agcaggcgct gcgcttcacg ctttccgagg accttacggc ggccattcct cccggcgacg
     6961 tttcgctgta tcgcatggcg ctggacctgg cggccggatt taagccgctc agccaggcgg
     7021 agcgcgacga gctgctggcg agcgcccagg gactcgatcc gctcttccgg gcatgaggcg
     7081 cttacctcat ttcccggtcc tcatcgtcta tatcgagcga taacggcgca aacagcgcgg
     7141 ttttgggcgc cgggtagggc acgtcccaac ccttgatgct gtgccataga atgcgattga
     7201 gtgtttcggt gggcgcggcg tccggcacgt cccagcgcat acgcgccgat gccagcgcac
     7261 cctctcgcgc cttgccccgc agcgccttcg cagggggatt gagctcgaaa agcgattgct
     7321 gcggctcgac ggcggagtac ggctccagat tcggcgtgtc ctgaaaactg gcgcgcatgt
     7381 cgttcgcaat cagatcgaac agggacatgg tgggcaatcc gaggatcagc tcgatcgtct
     7441 ttaacatgct ctggtgcgaa taaaacgtgg aatcgacgtg accgcggcgc acatagggac
     7501 tgacggccag cgccacggta cgatgcccgt ccacgtggtc tactccattc tgggcatcgt
     7561 cttccaccac gagaatggcc atcttactcc agaagcggct cttagatagc gcttccacga
     7621 tttggccgag cgcaaggtca ttgtccgcca ccatagcctt gggagtggag acatcgggcg
     7681 ttgttccgaa cgtatggtcg cagggcaatt gaaggatggt gaggttgggc attttgccat
     7741 ctttctccca accggcgaaa tctttgagga agatctgcgc gcgcaccata tccggaatcg
     7801 cattggtgta ggtgggaaag tttcgcgcca gcagcttatc cagcggtggg atcggagaac
     7861 tgacgctcca ccggctgctg aagtcctccc cgcgcttcca cgcttcgagc agcccgaatc
     7921 gctcccggct cgtgggctcg ggcatgcgag gagcgaactc cccgtataca cgcacggatt
     7981 tccctgcttt caacgccagg tcccacagga agcctccctc cgagtaagca atggggtcgg
     8041 tgccgtcgaa cggatagctt cggccgatgt agccgggcca cagggcgtac gcggtctgat
     8101 tggcctgcgt tacccattgg tgaccatcgg cgctgtttcc tccggtggca tagaaattat
     8161 cgaggagcac aaactgctcg gccattcgac ggtggttcgg agcgacgggc tcaccaaaca
     8221 tcaccaggcg aggctctcca ttgccttttc ccaggtcgcc gaatagctgg tcgtaggtgc
     8281 ggttttcttt gatgacgtag acgacgtgct cgatcagcga ggggtcgccc gaacgcaacg
     8341 gaatggcctt cggagcaact cgaacgttcg ccggacgggc agcggggcgg acggcgccca
     8401 cgggttgcat acgattgttt tcggcgaccg cggtcgtgta gtttgccagt tgcgcctcat
     8461 ccggaagcgg aatcacggag acggcgcctc gataggcatg cacaaaacgt ctgtccgggc
     8521 tgccctgcca tccggagcct acccccaaca aggcggtcac tgccagatgt tttccatcgg
     8581 gactcaaagc caggtcattc ggataccagg cggtcggaat cgcacccagg aagcggccgg
     8641 aagccgggtc cagaaccaca accgcgttga ttcctccaca ggccacatag agcctgcgct
     8701 tgtcgctgga caggcggatc gccgtcggag caattccctt cacttccgtg tggaaaggtt
     8761 tgagggagat cgtctcgacc acggcatgct tgcgagtgtc gatgacagag attgaatcgg
     8821 agttcgaatt gactac
//
LOCUS       DASTMK010000113         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_486099, whole genome shotgun sequence.
ACCESSION   DASTMK010000113 DASTMK010000000
VERSION     DASTMK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486099"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VFQ79_04585"
     CDS             <1..929
                     /locus_tag="VFQ79_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138958.1"
                     /translation="YMETFNLTLEHEVARNLVARASYIGNLGRRLSYTDDLNYARYAP
                     GATTGNIQQRRPYGDFGAILTTYSAGTSSYHGLQLSMERRVTRGFSFEVNYTFSKSID
                     ETSADTTPQNPSQTVPMNRTLNRGVSDFDQRHRLIASHVWTLPELSGQNALVRGVLGG
                     WQFSGITTIRSGGPFSVMSGTDRAFSGLGSTFADVIGDPFLDSGRPRAERIERYFNTS
                     AFTLAAPGTFGTSPRNTMRGPGAINFDFGLMKIFPVTEQVRLQFRAEMFNAFNTPSFN
                     NPFANVNTPSRFGRIEGAGSPRIMQFAMKISF"
     gene            981..1868
                     /locus_tag="VFQ79_04590"
     CDS             981..1868
                     /locus_tag="VFQ79_04590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0138959.1"
                     /translation="MVIRSFSRAVALLVARILAFLALVVLAMGFLMAVLRNARERLLA
                     PSDFDTLALGAIAGLAAALAATLLCMKFFERLRWVDVGLWWRPPAQWNLAVGLFAGLV
                     SASVATVVPVLFGAGEFLPVASSNADLPQLIYFLVVITIAATGEELQFRGYLFQVLVR
                     ATGITPALLITGVLFGMLHLGNPGSSRLGTLNTVLAGVVLGYAFWRSGDLWLPIGAHI
                     GWNCVFPLVGDELSGIGVHLTGYRIFWKSAVLWHGGSYGPEGGLYGTAGLLAMLVLVR
                     RLPVKRQDAPLLESGATPL"
     gene            complement(1855..2865)
                     /locus_tag="VFQ79_04595"
     CDS             complement(1855..2865)
                     /locus_tag="VFQ79_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0138960.1"
                     /translation="MRRRSFLGAVAGFPACRAKRPRLNVFNWSSYVARNTIPDFEREF
                     DVDVRYSIYESNEEMLARVLSGNSGWDVVFPSNYLILPMRENNLLAPLQHGWLPNADH
                     LDRRFQNPVWDPALDYSIPYMWGASGILFNRSLSPAPASWADLWKPRLNGRITMLDDP
                     AEVLGACLKKIGESINATDPAVLLRAKEEAVRQKRLLRAYLNAEVRDQVVAGDVLAAQ
                     LWATTAQQAMDAASNLAFAYPSEGFAVYADTAVILRESGRVRLAHQFIDYLLRPEVAA
                     AVVRETRTATANRAARELLREVSPALYPADDVMARGEWFEALPAAAQRLRDRLWTEIK
                     AA"
     gene            complement(2862..3620)
                     /locus_tag="VFQ79_04600"
     CDS             complement(2862..3620)
                     /locus_tag="VFQ79_04600"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138961.1"
                     /translation="MKHWLPVHAAAVFTFLYLPLAVLVVFSVNSSRFTAWTGFSLDWY
                     REMLRNPQLAEAAWNSIVIAAVATAISVSAGTLAAYALWKRTSRLLAGAVYLSLVTPE
                     IVMGISLLAFFQWIFRHLDLRLGMHTVILAHVSFTIAYVIVVVLARLRTLDGTLEEAA
                     QDLGATEWQAFRTVTLPLLVPGIAAAGLLAFAVSFDDYVITSMVAGVDSETLPMVIYA
                     MARRGATPVLNAVSTAIVLVIGTLTLLSARLMKQ"
     gene            complement(3617..4447)
                     /locus_tag="VFQ79_04605"
     CDS             complement(3617..4447)
                     /locus_tag="VFQ79_04605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138962.1"
                     /translation="MNRLRALFLAPVWVIVGSLLFAPLAIVFLYSLLTRGAYGGLAPP
                     WTFENYQRLADSLYLSIAAQSFLLAGISTLLCLLIGYPLALFISRSGRRKNLYLLLVI
                     TPFWISFLVRTYAWMFLLRDTGLINTVLEAAGLIREPLPLLYNPAAVVLGLVYGYLPF
                     MVLPVYAALERLDPALLEAAADLGAAPRASTLRILLPLSWPGIRAGIILVFIPCLGAY
                     LTPDLLGGGKAMMIGNLVQNQFTTARDWPFGAAVSLALMMPVALLLVLLLRRKGEELL
                     "
     gene            complement(4444..>4769)
                     /locus_tag="VFQ79_04610"
     CDS             complement(4444..>4769)
                     /locus_tag="VFQ79_04610"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU0138963.1"
                     /translation="TGTPEEIYLKPATRFVASFVGHVNWIDGIGVRPEATRIGREAPA
                     TGARSVPATAVNCVFLGNCVQVEARLLSGEPVVVELPFGHERPTPGDTTHIWWRPEDE
                     LRFRE"
BASE COUNT          925 a         1506 c         1475 g          863 t
ORIGIN      
        1 gttacatgga gacctttaac cttacgctgg agcacgaggt cgcgcggaac ctggtcgccc
       61 gggcgagtta catcggcaat ctcgggcgcc ggttaagcta tacggacgac ctgaattacg
      121 ctcgctatgc tccgggcgca accaccggaa acattcaaca gcggcgtcct tacggcgact
      181 tcggcgctat tctcacaacc tactctgccg gcacatcgag ttatcacggg ctgcaactgt
      241 cgatggagcg ccgtgttacg aggggtttct ccttcgaggt taattacacc ttctcgaagt
      301 cgatcgatga gacatcggcc gatacgaccc cgcaaaatcc gtctcaaacc gtccccatga
      361 accgcacgct caatcgggga gtttcggact tcgaccagcg gcaccggctc atcgcctcac
      421 acgtctggac tttgccggaa ctctcggggc agaacgcact ggtgcgcggg gtgctcggtg
      481 ggtggcagtt cagcggcatc acgacgatcc gaagcggtgg tccgttctcg gtcatgtccg
      541 gtaccgatcg cgcgttcagc gggctcggct caaccttcgc cgacgtgatc ggcgacccct
      601 tcctcgacag cggaaggccg cgggcggaac gtatcgagcg ctacttcaac acctccgcgt
      661 tcactctcgc cgcgcctggc acgtttggga catctcctcg caatacaatg cgcggaccgg
      721 gcgccatcaa cttcgatttc ggtctgatga agatattccc ggtgacggaa caggtccggt
      781 tgcagttccg cgcggagatg ttcaacgcct tcaacacgcc gagcttcaac aacccgttcg
      841 ccaacgtcaa cactccgtcg cgcttcggac gcatcgaagg cgccggttcg ccgagaatta
      901 tgcagttcgc catgaagata tccttctgag ggccgcccgg aaaccgagcc gcgcgcgccg
      961 gcaaacgcta ggctcaagaa atggtgattc gctcgttttc ccgggccgtc gcgctactgg
     1021 tcgcgcgcat cctggcgttc cttgcgctgg tggtgttggc gatgggtttc ctgatggctg
     1081 tgctgcgtaa tgcgcgggaa cggctgctgg ccccatcgga tttcgatacc ctcgcgctgg
     1141 gggccatcgc cggacttgcc gccgcattgg ccgccaccct cctctgtatg aagtttttcg
     1201 agaggctgcg ctgggtggac gtgggcctct ggtggcgccc gccagcacaa tggaatctgg
     1261 cggtgggttt gtttgccggg ctggtatctg ccagcgtcgc tacagtggtt cccgtgctgt
     1321 tcggagcggg cgaattcctg ccggtagcct cctcgaacgc cgatctgccc caactcatct
     1381 acttcctggt ggttatcacc attgctgcca ctggcgagga actgcaattc cgtggttacc
     1441 tgtttcaggt gctggtcaga gccaccggca tcacccctgc cctgctgatc accggtgtgc
     1501 ttttcgggat gctccacctt ggcaaccccg ggtcctcgcg cctgggaacg ttgaacactg
     1561 ttctggcggg cgtcgttctg ggatacgcgt tctggcggag cggcgacctg tggcttccca
     1621 tcggagcgca tatcggatgg aattgcgtct tccccctggt gggcgatgag ttgagcggta
     1681 tcggagtgca tttgaccggg tatcggatct tctggaaatc cgccgtgctg tggcatggag
     1741 gctcgtacgg acccgaaggc ggactctatg gtaccgccgg attgctcgcc atgctggttc
     1801 tggtgcggag gctgccggta aagcgccagg acgctccgct gctggaatcc ggcgctacgc
     1861 cgctttgatc tcggtccaaa gacggtcgcg caatcgctgt gccgctgccg gaagggcttc
     1921 gaaccactcg ccccgggcca tcacatcgtc tgcgggatac aaggccggac tcacctcccg
     1981 cagcagttcc cgcgcggcgc ggttcgccgt cgccgttctg gtctcgcgaa cgacggcggc
     2041 cgccacttcc ggccgcagaa gatagtcgat gaactggtgc gccagccgca cccgtccgct
     2101 ctcgcgcaga ataacggcgg tgtcggcgta gacggcaaag ccctccgacg ggtaggcgaa
     2161 tgcgagattc gaggccgcat ccatagcctg ttgcgcggtt gtggcccaga gttgcgcggc
     2221 gagcacgtct cccgcaacca cctggtcgcg cacctcggcg ttcaggtagg cgcgcaacaa
     2281 tcgcttctgc cggacagcct cctctttcgc gcgaagcagc accgccgggt ccgttgcgtt
     2341 gatcgattcc cctattttct tgaggcaggc gccgagcact tccgccggat cgtccaacat
     2401 cgtgatgcgc ccgttgagcc gcggcttcca gaggtccgcc caacttgcgg gcgccgggga
     2461 tagagaacgg ttaaacagaa tcccgctggc gccccacata tacggaatgc tgtagtccag
     2521 ggctggatcc cacaccgggt tctggaaacg ccggtccaga tggtccgcat tcggcagcca
     2581 gccgtgctgg agaggcgcca gcagattgtt ttcgcgcatg ggcagaatca gatagttgct
     2641 cgggaagacg acatcccagc cgctgttgcc ggagaggacc cttgccagca tctcctcgtt
     2701 gctctcatag atgctgtagc gcacatcgac gtcgaactcg cgctcgaaat ccgggatggt
     2761 attgcgcgcg acatagctcg accagttgaa gacgttaagc cgtggccgtt tcgcacggca
     2821 ggccggaaag cccgcgacag caccgagaaa gctccggcgt ctcattgctt catcaacctt
     2881 gccgaaagca gagtaagggt tccgatgacc agcacgatcg ccgtcgaaac cgcattcaga
     2941 accggtgtcg ccccgcggcg tgccatcgca tagatcacca tcggcagcgt ctcggaatcc
     3001 acacccgcca ccatactggt gatgacgtag tcgtcgaagg acacggcaaa cgccagcagt
     3061 ccggctgcgg cgattccggg aacgagcagc ggcaacgtga cggtgcgaaa cgcctgccat
     3121 tccgtggcgc cgagatcctg cgccgcctcc tcaagtgtgc catcgagtgt ccgcaagcgc
     3181 gccagcacca ctacaatcac gtaggcaatg gtgaacgata cgtgcgccag aatcaccgtg
     3241 tgcattccca gccgtaagtc gaggtgccgg aagatccatt ggaagaaggc gaggagggaa
     3301 atgcccatca ctatctccgg tgtgaccagc gacagataca ccgcacccgc gagcaggcgc
     3361 gatgttcgtt tccacagcgc atacgccgcc agcgtaccgg cggagacgga aatggccgtc
     3421 gccaccgccg cgatgacgat gctgttccag gcagcttcgg cgagttgcgg gttgcgaagc
     3481 atctcccgat accagtcgag cgaaaagccg gtccaggcgg tgaaccggga cgaattcacg
     3541 ctgaagacca ccagaaccgc cagcggcaga tagagaaacg tgaacaccgc ggccgcatga
     3601 accggcagcc aatgcttcac agcagctcct cccctttgcg gcgcagaagc aggacgagca
     3661 gcagcgcgac cggcatcatg agcgccagcg acaccgcggc accaaacggc cagtcgcgcg
     3721 cggtggtgaa ctggttttgt accaggttgc cgatcatcat cgctttgcct ccgccgagca
     3781 ggtccggtgt gagataggcc ccgaggcagg ggataaagac caggatgatc ccggcgcgga
     3841 ttcccggcca gctcagcggc agcaatatgc gcagtgtgct cgcgcggggc gccgctccca
     3901 ggtcggctgc ggcttcgagc agtgccgggt ccagccgttc gagcgcggca tagacaggca
     3961 gcaccatgaa gggaagatag ccgtagacaa gcccgaggac cacggcggcc gggttgtaca
     4021 acaacgggag cggctcccgg atgaggccgg cagcttcgag gacggtgttg atgagccccg
     4081 tatcgcgcag caggaacatc caggcgtagg tccgtaccag aaagctgatc caaaacggcg
     4141 tgatgaccag cagaaggtac agatttttcc gccgtccgga tctcgaaatg aacagggcca
     4201 gcggatagcc gatcagcagg cacaacagcg tggagatacc ggccagcaga aacgactgcg
     4261 ccgcaatact gagatacagg gaatcggcca gccgctggta attttcgaaa gtccagggcg
     4321 gtgccaagcc gccgtacgcc cccctggtaa gcagactgta gagaaagaca atcgccagcg
     4381 gcgcgaagag cagagacccg acaatcaccc aaacgggcgc gagaaacagg gcgcggagac
     4441 gattcattcc cggaaccgaa gctcgtcttc gggacgccac cagatgtgcg tggtgtcgcc
     4501 cggtgtgggc ctctcgtgcc cgaagggcag ttcgaccacg acgggctcgc cggaaagcag
     4561 gcgggcctcc acctgaacac agttgcccag aaagacgcaa ttcaccgccg ttgccggcac
     4621 acttctcgct cccgtcgcgg gcgcctcgcg tccgatccgg gtggcctcgg gacgaacgcc
     4681 gatgccgtcg atccagttga catgcccgac gaaggaagcc acgaatcgcg tagccggttt
     4741 caggtagatc tcctccggtg taccggtct
//
LOCUS       DASTMK010000114         8695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_294543, whole genome shotgun sequence.
ACCESSION   DASTMK010000114 DASTMK010000000
VERSION     DASTMK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294543"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1299)
                     /locus_tag="VFQ79_04615"
     CDS             complement(103..1299)
                     /locus_tag="VFQ79_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU0138964.1"
                     /translation="MPDYPPLNPPKRLLYGPGPTMVEPRVYDAMSKPIVGHLDPYFFT
                     VFEDIRKLLAQVFGTSNDFTMVISGTGSGGMEAAVVNFVEPGTKFAVLANGYFCDRIS
                     EMAGRHGANVVRLEKPWGEVFEDGEVRDFIHREKPAVVAYVNAETSTGALQSGAGISR
                     AAHEVGAVVIADCVTSLGAMPIMMDNTGIDIAYSCTQKGLSCPPGLAPISVSARAMER
                     LRARKRPVDTWYLDLRLLDEYYEGSHRYHHTAPISMFYALREALTLIAEEGLENRWKR
                     HLRCHLALVSGLEAMGMTMHVADGHRIWNLNTPRVPEGVDDAKVRRRLLESHGIEILG
                     GFGPLAGKIFRIGIMGPLATEESILSLLEALETALQAEGYKAKESGRAAAREAFADLD
                     RKPQPA"
     gene            complement(1335..2474)
                     /gene="ribB"
                     /locus_tag="VFQ79_04620"
     CDS             complement(1335..2474)
                     /gene="ribB"
                     /locus_tag="VFQ79_04620"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEU0138965.1"
                     /translation="MPFASIPEALDDFRQGRMLVVVDDEDRENEGDLTIAAEKITPDI
                     INFMATHGRGLICLALSAERCDELRIPLMSPNNTSKFGTAFCEAIDAREGVTTGISAA
                     DRAHTIRISMDPATKPEDLARPGHVFPLRAREGGVLVRAGQTEAAVDLARLAGLSPGG
                     VICEIMNNDGSMARVPELENFCRLHDLKMISVAELIRYRLQHEKFVRRMAEGCLYTPF
                     GEFKTIAYSSALERETHLALVRGSVAGTENVVVRMHSHCVYGDVFGSVDCECRSLIEH
                     SLGRIAKEGAGVLVYLHQTGPGVRLQYTGAELPRLVSHGRDFMHYTTPDGQRLLQYES
                     GIGAQILSDLGLSTIRLLTNHPRKIVGLEGFGIEIVEQIPVHEPY"
     gene            2689..5007
                     /locus_tag="VFQ79_04625"
     CDS             2689..5007
                     /locus_tag="VFQ79_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0138966.1"
                     /translation="MSLQSDGANFGFTRSDNNRTVFSYPMYAALRDHNEVFSGLIGRT
                     GSPATLSWGGDAARAAAEIVTGNFFSVLGVKPALGRFFMPEDETQRNSVIVLSHGYWT
                     GRLGQDPKVLNSRVLMNSHPVLVIGVAPQGFQGLVSGQSPDFFAPLSLMPMISPGWQR
                     NEQVDAYWLNLIGRLKPGVSPQQASAALLPSFRAILQDHLQRMSDVTEEVRTTARSKP
                     LRIEPASQGINVLRDQWQSPLMVLLAMVGLVLLITCSNLASLMVVRATARRREIAVRF
                     AVGASRWQVARQLMVESVTLALAGGLLGLFVCGALTEGLLRLLPENVAGGWLTSNMDL
                     RLLAFSMALGMAAGVLFGLIPALRALKADLAPALKEHSSGLTAADHQSRSRQVFVAAQ
                     ICFSLLLLAGAGLFTHTLLNLMSTDPGFRTGHLITFSLDPSLSGHPRSRALALFRELE
                     DRLRALAPVKSVARAAYQPLGGSTVVNGIKAPGTRAASDKYVDCGQNAVSAGYFQTLG
                     IPLLAGREFNPRDNEKAPKVAILNETFARFLFEGQNPIGRSIHVGSDDADLSIVGVVS
                     DSKYGDIREKSTRFLYVPYEQAGQIITQQAVFFLRAQGDEQQVMVAARGAAKETAAGV
                     PIERMTSMDSTLARSIYKERLIASLAIAFGLLATILAAVGLYGAISYSTERRTREFGI
                     RLALGANPKSLLALVMREAAWMIAVGIAAGLPLSYALAKAVQSQLYGVAAHDPWAFVG
                     AALLIAAVTCCAALSPSLRAMRIDPVRALRYE"
     gene            complement(5019..5807)
                     /locus_tag="VFQ79_04630"
     CDS             complement(5019..5807)
                     /locus_tag="VFQ79_04630"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0138967.1"
                     /translation="MQLDFSGKTAVVTGGANGIGLAAARALAQGGASVWIFDLEKEKP
                     SEVAAEFGARGCVMDVTSRESLEAAFVQSGVPDVVVANAGTVWFRDIFSTSPEVWERT
                     LAVNLSGVFYTVQVAAGLMKPRRSGSIVLTASTNSYDGEAELMAYNVTKAGLLGLLHT
                     AANEFGPHQIRINAVCPGLIRTRLTTSFYDNPAVIKEYFRQIPLGRGGETSEVANAIA
                     FLASDLASFITGATLFVDGGQMSAKFGTWNDDTGEFVKDRWRLK"
     gene            complement(6862..7284)
                     /locus_tag="VFQ79_04635"
     CDS             complement(6862..7284)
                     /locus_tag="VFQ79_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138968.1"
                     /translation="MLTSTIWGRVNPRVAMPILLGVAFVMCAAALLLYVALVRPAARV
                     SESGVITSKQILPARTIVRRQASPRREMWTTNEFELPDRIEFQIRLEGSSEQVRYALP
                     VNAAAGYEVGQQVNVTYERRSIPLIWSRVYVRGIAAAE"
     gene            complement(7312..7827)
                     /locus_tag="VFQ79_04640"
     CDS             complement(7312..7827)
                     /locus_tag="VFQ79_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138969.1"
                     /translation="MPGLEPMLTALPMSKRLAFAAACGERILNEVANLGEEIPGEEPV
                     RRGIDFGWRAAGGETLDRAEAEAIYENVRNAIPITEDSSEFEMACAGAGGIPALMALE
                     PDDSASHAASVAEQMVSLVGLYYEDSRRVELEEERWQRTLIERLESAPGRVTREVVNT
                     VPDYDRGERVE"
     gene            complement(7865..>8695)
                     /locus_tag="VFQ79_04645"
     CDS             complement(7865..>8695)
                     /locus_tag="VFQ79_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEU0138970.1"
                     /translation="GLDANPEAPARYFLFTNQIGAPERVEDDSGRTAWRAEIRAYGQC
                     RVDPDSTLELALRFPGHYYDRETGLHYNRHRYYSPELGRYLQPDPLDLAGGLNLYAYP
                     SQPLNLVDVDGLRCPHMSPRRLRKLRHRQRNLARNLRKRIMRIPFEKDGRTYVVATVR
                     HRQTGEVRTVVTSNVGPKDPNRRTPHPYARDAGVTDEMYVRPQTRRKGEAEHAEDRLT
                     TWAQENDYDVVSLTPTRPCCEGCTSIVHNRLNDPGLDRVDRLPNMPKSRSQPSRGDPG
                     "
BASE COUNT         1671 a         2694 c         2562 g         1768 t
ORIGIN      
        1 ggcgccaccg ccgcgctgac ggtgatcatg ggccgcgaag cgatctaccg gcggcgcatg
       61 gtgacgtgga aagaactggg cgtagaggtc tgagcccggc tctcaggcag gctgcggctt
      121 ccggtcgagg tcggcaaacg cctcgcgcgc cgcggctctg ccgctttcct tcgccttgta
      181 accttccgcc tggagagcgg tctccaacgc ctccagcaaa gacaaaatgc tctcttcggt
      241 ggccagcggc cccataatgc cgatgcggaa gatctttccc gcgagcggcc cgaagcctcc
      301 caggatctcg ataccgtgcg actcgagcag ccgcctccgg accttcgcgt cgtccacacc
      361 ctccggcacg cgcggcgtat tcagattcca gatgcgatgg ccgtcggcta cgtgcatcgt
      421 cattcccatc gcttcgagcc cggacaccag ggcgaggtgg caccgcagat gccgcttcca
      481 gcgattctca agaccttctt ccgctatgag cgtgagcgct tcccgcagcg catagaacat
      541 cgatatcggt gccgtgtggt gatagcgatg cgagccctcg tagtattcgt cgagcagacg
      601 caggtcgaga taccaggtat ccaccggacg tttgcgcgcg cgcagccgtt ccatggcgcg
      661 ggcggaaacg ctgatcgggg ccagtcccgg cggacagctc agaccttttt gcgtgcaact
      721 gtacgcgatg tcgatgccgg tgttgtccat catgatgggc atggcgccca gcgaagtaac
      781 gcaatccgcg ataacgacgg cgcccacttc atgagcggct ctcgaaatcc ccgcgccgga
      841 ctgaagcgcc cctgtcgagg tctcggcatt cacataagcg acaaccgcgg gcttctcgcg
      901 gtggataaag tcacggacct caccgtcctc gaatacctcg ccccagggct tctccagccg
      961 gacgacgttc gccccgtgac ggcccgccat ttccgaaatg cggtcgcaga aatacccgtt
     1021 cgccagcacg gcaaacttcg tgcccggctc cacgaagttc acgaccgcgg cttccattcc
     1081 gccgctccca gtgccggaga tcaccatggt gaaatcgttc gacgtcccga acacctgcgc
     1141 caatagcttg cggatatcct cgaacaccgt aaagaagtac ggatccagat gcccgacaat
     1201 cggcttcgac atggcatcat agacgcgcgg ctcaaccatg gtcggcccgg gtccgtagag
     1261 cagacgcttg ggaggattca aaggagggta atcaggcata caaggatagt ctatccggtt
     1321 tccggcggct ttcttcaata cggttcgtgt acggggatct gctcgacgat ctcgatgccg
     1381 aatccctcca gcccgacaat cttgcgcgga tgattggtca gaagccgtat ggtgctgagg
     1441 ccgagatcgg agagaatctg cgctccgatg ccgctttcat attgcagcag ccgctgcccg
     1501 tccggcgtcg tatagtgcat gaaatcgcgc ccgtgtgaaa cgagccgcgg aagttcggcg
     1561 ccggtatatt ggagccgcac gccgggtccg gtctggtgca ggtacaccaa tacgccggcc
     1621 ccttctttcg cgatgcgccc aagcgaatgt tcgatgaggc tgcggcactc gcaatccacg
     1681 gaaccgaaaa catctccgta aacgcagtgc gaatgcatcc gcaccacgac gttctctgtg
     1741 cccgccacgg accctcgaac cagggcaaga tgcgtctcgc gttccagcgc actggagtaa
     1801 gcgatggttt tgaactcgcc gaacggagtg tacaggcagc cctcggccat gcgccggacg
     1861 aacttctcgt gctgcagccg gtagcgaatc aactcggcca ccgaaatcat cttcaggtca
     1921 tgaaggcggc agaagttctc gagttccggc accctcgcca tggagccgtc gttgttcatg
     1981 atttcgcaaa tgacgcctcc gggactgaga ccggcgaggc gggccagatc gaccgccgcc
     2041 tccgtctgtc cggcccggac cagaacgccg ccttcccgtg cgcgcagcgg aaagacgtgc
     2101 ccgggacgcg ccaggtcttc cggcttggtc gccggatcca tggagatgcg gatcgtgtgc
     2161 gcgcggtccg cggcggagat gcccgtggtg accccctcgc gggcatcgat cgcttcgcaa
     2221 aaggcagtgc cgaacttcga tgtgttgttc ggcgacatca gcggaatgcg cagctcgtcg
     2281 cacctctccg cgctcaaggc caggcagatg agcccccggc catgcgtagc catgaagttg
     2341 atgatgtccg gagtgatttt ctcggccgca atggtcaggt cgccttcgtt ctcccggtcc
     2401 tcgtcatcga caaccaccaa cattctgcct tgccggaaat cgtcgagcgc ttccggaatc
     2461 gaagcaaatg gcatattgtg gaactcttat tcaatatagc ctgtaccccc gaaggaggga
     2521 gcatctcatg tggaatgatg tgcggtttgc cctgaggacc ctacgccgct caccgggctt
     2581 cactcttgta gcggtgctgt ccctggcgct cggcatcgga gcgaatacag cgattttttc
     2641 tctacttcac caggttgctc ttcgctcgct tccggtccgt cccgtctggt gtcgctccaa
     2701 tccgacggcg cgaatttcgg attcacgcga agcgacaaca acagaaccgt attctcctac
     2761 ccgatgtatg ccgctcttcg agaccataac gaagtcttca gcggcctgat tggacgaacg
     2821 ggttcgccgg caactctgag ttggggcggc gatgctgcgc gcgccgcggc ggaaatcgtc
     2881 accggcaatt ttttctccgt gctgggggtc aagcccgcgt tgggacggtt tttcatgccc
     2941 gaagacgaaa cacaacggaa ttccgtgatc gttctcagcc acggatattg gaccggccgt
     3001 ctcggacagg accccaaggt tttgaacagc cgcgtcctga tgaacagcca tccggtgctg
     3061 gtgatcggcg tcgctccaca aggcttccaa ggtcttgtct ccggccagag tcccgacttc
     3121 ttcgcacccc tgtcgttgat gcccatgatt tcgcccggtt ggcagcgaaa cgaacaggtg
     3181 gacgcttatt ggctgaacct gattggccgg ttgaaaccgg gagtttcacc gcagcaggca
     3241 agcgccgcgc tgttgccttc cttccgcgcc attcttcagg accacttgca gcgcatgtcc
     3301 gatgtcacgg aggaagtccg cacaacagca cgctcgaagc cgctcagaat agaacccgct
     3361 tcccagggta taaacgtgtt gcgggaccag tggcagtctc cattgatggt gctgttggcg
     3421 atggttgggt tggtgcttct gatcacctgc tccaatctgg ccagcctgat ggtggtgcgc
     3481 gccacggcaa ggcggcggga aatagcagtg cgtttcgcgg tgggcgcgtc ccgatggcag
     3541 gttgccagac agttgatggt agagagcgta actcttgcct tggccggcgg cctgttgggc
     3601 ctctttgttt gcggagcttt gacggaagga ttgttaaggc tactaccaga aaacgttgcg
     3661 ggcggttggt tgacgtcgaa catggacctt cgcctgttgg ccttcagcat ggccttgggg
     3721 atggcggcgg gtgtgctttt cgggctgatt ccggcgcttc gcgcgctcaa agccgatctt
     3781 gcaccggcgc tgaaggagca tagttcagga ttgacggccg ccgatcatca atccaggagc
     3841 cggcaggtgt ttgtggcagc acagatctgc ttttccttgt tgctgctcgc aggcgccggc
     3901 ttgttcacgc acacgttgct gaacttgatg tccaccgatc cgggtttccg gaccggtcac
     3961 ctgattacgt tttcgcttga tcccagcctc agtggccacc ccaggagccg cgcactggct
     4021 ctgttccggg aactggagga ccgcttgcgc gctctggccc ccgtgaaatc ggttgctcga
     4081 gccgcctacc agcccttggg cggttctacc gtggtcaacg gcataaaggc tcccggaacc
     4141 cgcgcggcga gtgataagta cgtcgattgc ggacagaatg ccgtcagcgc cggctacttc
     4201 cagacacttg gcatcccact gctggctgga cgggagttca acccccggga caatgaaaag
     4261 gcaccaaagg tcgcgattct caacgagaca ttcgcgcgtt ttcttttcga aggccagaac
     4321 ccgatcgggc gttccataca tgtgggctcc gatgacgcgg acctgagcat tgtgggtgtc
     4381 gtaagcgatt ccaagtacgg cgacattcgg gaaaaatcca cgcgattcct gtatgtgcct
     4441 tacgagcagg ccgggcagat aatcacgcaa caggccgtat tttttctccg ggcacaagga
     4501 gacgaacagc aggtgatggt ggccgcccga ggggctgcga aagagacggc tgccggcgtt
     4561 cccatcgagc gcatgacctc gatggactca acgctggcgc gttccatcta taaggaacgg
     4621 ctcatcgcga gcctcgctat cgcgttcggg ctgctcgcca cgatcctggc ggcggtcggg
     4681 ttgtatgggg cgatctccta ctcgacagag cgccgcactc gtgaattcgg aattcgcctg
     4741 gcgttgggcg cgaatcccaa aagcctgctg gcattggtca tgcgtgaagc agcctggatg
     4801 attgccgttg gtattgctgc cggattgcct ttaagctatg ccctcgcgaa ggcagtgcaa
     4861 tcgcagttgt atggcgtcgc ggcacacgat ccgtgggctt tcgtcggagc ggctctcctg
     4921 atcgcagccg taacctgttg cgccgcgttg agtcccagtt tgcgcgcgat gagaatcgat
     4981 cctgttcgcg ctctgcgata cgaataggat ctgcgatttc acttcaaacg ccagcggtct
     5041 ttcacgaact cgccggtgtc gtcgttccag gttccaaact tcgccgacat ctggcctcca
     5101 tccacaaaga gggtggcgcc ggtgatgaac gacgccaggt ccgatgccag gaacgcgatg
     5161 gcgttggcta cttccgaggt ctcgcctcca cgtccgagcg ggatctggcg gaagtattcc
     5221 ttgatcacgg cgggattgtc gtagaaggaa gtcgtcagcc gggttcggat gaggccggga
     5281 caaacggcgt tgatgcggat ctggtgcggc ccgaattcat tggcggcggt gtgcagcaac
     5341 ccgagcaggc cggctttggt gacattgtag gccatcaact cggcctctcc gtcataggaa
     5401 ttcgtggaag ctgtaaggac gatcgatccg gaacgcctgg gtttcatgag acccgccgcc
     5461 acctggaccg tataaaagac ccctgagaga ttgaccgcca gcgtgcgctc ccacacctcg
     5521 ggcgaagtcg agaaaatatc gcggaaccac accgttccgg cgttcgcaac cacgacgtcc
     5581 ggcacacccg attgcacaaa cgccgcttcg agcgactcgc ggctggtcac gtccatgacg
     5641 catccacggg cgccgaactc cgccgcaact tcggaaggtt tctctttctc cagatcgaag
     5701 atccagaccg atgcccctcc ctgggcgagg gcacgcgccg ccgccagacc gattccattc
     5761 gctcctccag ttacaaccgc ggtcttaccg ctgaagtcca gttgcatata acgtgtgttc
     5821 taccacactg tttgtgataa cgttgtgctg agtggaaaag gtggccctcc acgagccgcc
     5881 aggttgacgc ccaagcaatc gagtgaaagc gcacgcaagg cagttctgac acgatgggcg
     5941 aaagccgaaa aatggaaatc aaaacgacct cgacgaacgc atccgacaaa gctgtcttgg
     6001 ccttgttgaa gcgcatcaag gaaaccaaca accaaagcga gctccgtcaa ctttccgatc
     6061 aactcgagcg actaattttt cacaagcaag ccgccaatag ctaacggact tccgagtctg
     6121 gcttcctcgt tcctgcgcag gttcacgagc taccgttcaa tgccaagttc aagttgcctc
     6181 caccgaagag aagtcgcgat ggcaagtggt tcaaacgctt caagagcggc tttctcgccg
     6241 gtaatggaga acgcgtatcg acattcctgg agccgggtat ggctgtggaa ggcgacgagg
     6301 ttcagtgagc cggcgggcca ttactccgcc gcaactacgt gtcttctacc acactgtttg
     6361 tgataaaagt gattcggact cttacatgtc tttaagtgat attatccaga gtttaggagc
     6421 ctgaaaggcg aggttgtcct accatcatgg atggtctcct gattcgtagc cccttcatcg
     6481 aagagattct ctcaggaata aagacctggg aaattcgtgg gtccaatacc aggaagcgcg
     6541 gtatcatagc gctgattagg gccggctccg gcctgatcgt tggcacctgt gagttagtcg
     6601 acgttcaagg gccgcttacg cttgccgaga tgcgacgtaa cacggccaga catcgcattc
     6661 cggcagccta tctacgcgat agcctcccgt atgtgagaac gttcgcgtgg gtcttgcgtg
     6721 acgcaaagcc cctcaagaag tcggtgccgt atgaacaccc gtccggtgca gtaatctggg
     6781 tgaagttgcc cgagttccgg gtagccgccg cacggtgacg gcggagttct tagctggcta
     6841 caatacgcac ggccctgcgc ctcactccgc cgccgcgatt ccgcgcacat acacgcggct
     6901 ccaaatcagc gggatactgc gtcgctcata ggtgacgttt acctgctggc ccacctcata
     6961 gccggccgcg gcgtttaccg gcaatgcgta tcgcacctgc tcggaggatc cctccagtcg
     7021 aatctggaat tcgatgcggt cgggcagttc gaattcattc gtcgtccaca tctctcttcg
     7081 cggcgacgcc tgccgacgga cgatggtgcg ggccggaaga atctgcttgc tggtaatgac
     7141 gccggattcc gagacacggg ccgccgggcg gaccaatgcg acatacagca gcagggccgc
     7201 cgcgcacatc acgaacgcca caccaagaag aatgggcatc gccacccgcg gattaacccg
     7261 gccccagata gtagaagtga gcattcgatg gcgtcccttc cgcgccgcgg atcactccac
     7321 gcgttctccg cgatcgtagt ccggcaccgt attcacgacc tcccgtgtca cgcgccccgg
     7381 agcggactcc aaccgttcga tcagcgtccg ctgccatcgt tcctcctcca attccactct
     7441 ccggctgtct tcgtagtaaa ggcccaccag gctgaccatc tgttccgcca cgctcgcggc
     7501 atgagacgcc gaatcatccg gctccaaagc catcagcgcc ggaatgcctc ccgctccggc
     7561 gcaagccatc tcgaactcgc tcgagtcttc ggtaatcgga atcgcattgc gaacgttttc
     7621 gtaaatcgcc tcggcttcgg cgcggtcgag ggtttcaccc ccggccgcgc gccagccgaa
     7681 atcgattccc cggcggaccg gctcttcgcc cggaatctct tcgccgagat tcgcaacctc
     7741 gttcagaatg cgctcgccgc acgcggccgc aaacgccaga cgtttggaca tcggcaaagc
     7801 cgtcagcatc ggttccaatc caggcaccag ctttcctcct ccaaagaatt atctccgata
     7861 accatcagcc cgggtctccg cggctcggct gcgagcggga cttgggcata ttcggcagcc
     7921 ggtccaccct gtcgagcccc gggtcattca agcggttgtg cacaatactc gtacaccctt
     7981 cgcagcacgg acgtgtcggg gtaagcgaga ccacgtcgta gtcattctcc tgcgcccatg
     8041 tggtcagccg gtcctcggca tgctccgcct ctcccttgcg ccgggtttgg ggccggacat
     8101 acatctcatc cgtgacgccg gcatcgcggg catacggatg cggtgtgcgg cggttcggat
     8161 ccttcgggcc gacattggaa gtaacgacgg tccggacctc tccggtttgg cgatgccgga
     8221 ccgtggcgac cacataagtt ctgccgtcct tctcgaaagg gatgcgcatg atccgcttcc
     8281 ggaggttgcg cgccaggttc ctctggcggt gcctcagttt ccggaggcgg cgcggcgaca
     8341 tgtgcgggca gcgcagcccg tcaacgtcca ccagattgag cggctggctg ggataggcgt
     8401 aaaggttgag acctcctgcc aggtcgagag ggtcgggctg aaggtatctt ccaagctcgg
     8461 gactgtaata cctgtggcgg ttgtagtgaa gcccggtctc ccggtcgtag tagtgaccag
     8521 ggaaacgaag cgcaagctcg agcgtgctgt ccggatctac gcggcactgc ccgtacgcgc
     8581 ggatctccgc tcgccaggcg gttcgtccgg aatcgtcttc cacgcgctcc ggcgcgccga
     8641 tctggttggt gaacagaaaa taccgcgcag gcgcctccgg gttcgcgtcc agtcc
//
LOCUS       DASTMK010000115        20248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_614546, whole genome shotgun sequence.
ACCESSION   DASTMK010000115 DASTMK010000000
VERSION     DASTMK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20248
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614546"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFQ79_04650"
     CDS             <1..609
                     /locus_tag="VFQ79_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138971.1"
                     /translation="PEKLIGFCSVDPQDPDAVEQLDHCVQDLGLRGLKMGPIYQNVAP
                     TDSRFRRILHRAEELRIPTLIHQGTTFCTNVSLEIANPIQLQPLALEFPRLKMVIAHL
                     GHPWVNETLVLIRKNRNVFADISALYYRPWQFYNALVSAMEYGVLDRLLLGSDYPFTT
                     PASTIDALRAVNRMVEGTHLPRIPEAKIEALIARDTLDLLGL"
     gene            complement(626..1777)
                     /locus_tag="VFQ79_04655"
     CDS             complement(626..1777)
                     /locus_tag="VFQ79_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0138972.1"
                     /translation="MKITNVEVLVLKSRGLYNRPEGAEEPQGPTYMGLVRVSTDAGIT
                     GYSDMETSAPVAKAAVEAPSWSEAGVECFEGLANLIVGENPLEVERLWYKMYRGSIYY
                     GRRGVAIQAISAIDIALWDIMGKFYGQPVCVLLGGKWRNRVRAYASTLFRPTPEAMGR
                     AARGYLDLGFTAVKFGWGVFGEDPRRDVELVEAARAELGDRNDLLVDTGWFIERTPKQ
                     AIQVIRSLEPYRPFFVEEILHPEDYDGYRRVAAEVETLIACGEQEATEWGFRELIERA
                     RVDILQPDLTRCGGFTVARKIVHMAEMANVLVIPHSWSSDLLTAASLHLNAFQRRAVF
                     VEFNTSQGPLSREMVKEPLQLEDGYLRVPEAPGLGVEVNEDTIERYRIA"
     gene            complement(1779..2789)
                     /locus_tag="VFQ79_04660"
     CDS             complement(1779..2789)
                     /locus_tag="VFQ79_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU0138973.1"
                     /translation="MLLHDPAKIDFSLPGKHHYQAAFHLDSAWGYSLVPVTVINGLRG
                     DGPAGVAVFGGTHGNEYEGQVAVKRLCRDLDPERMRGCAILIPQLSESACRANLRISP
                     LDGVNMNRAFPGNPRGTLSYRIAHFVKTCVFPRVRIVLDIHSGGNEAVFPLCTSLHPR
                     PDPAQQAEMVEVAKLFDPPFIFVYSRQMASGLLTDEAEDEGKIAIGGEFGFAESASPK
                     GVRHAYEGVKNVLRHYGLLEGEVEPIDTARGTPPRLVSAPELRDYIPCPRDGVWEPLF
                     QPGADVEAGQLIGRIHDFSDHASDPLEVTAHRSGVIIAQYSPAVCRKGLTLFVIAQDV
                     IL"
     gene            complement(2940..5108)
                     /locus_tag="VFQ79_04665"
     CDS             complement(2940..5108)
                     /locus_tag="VFQ79_04665"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HEU0138974.1"
                     /translation="MRSVGPLVVCVFILRAALAGQPGEANGTRQTVAVRISERIHVDG
                     VLSERVWRENGGIGELVQREPVPGVRPSEATEVHVAYNDDAIYVAIRCYDSRPGELRA
                     TQMQRDGDLLFDDYVQILFDTYADRRNAYYFATNPAGVMVEGTISENNTPNLNWDGIW
                     DVRPQRDSEGWSVEMEIPFKTIAFNPGSRTWGFNISRRIARLREESRWTSSNLDSEFF
                     LVARAGDVNGLEGLTQGIGLDVRPYALGGYSRDIGLRDRHQFARDAGADIFYRITANL
                     VSSTSINTDFAETEVDTRQINLTRFPVLFPERRQFFLEDSGIFEFAASPGTRDFTPYF
                     SRRIGLVDGLEVPILFGQKVTGKIGRFDVGLMDVKTRDSEVAPGRNFFVGRTKANFWS
                     QSYIGALVTDGEPTGKLENRQVGVDLKIATADLFKTGKNFRSLFYGSKTSTTGRKGDD
                     MFWGGEVVYPNDLLYVWYRWRFIGKNYNPALGFMRRTGVRESAMGSNFRPRPDFWNMR
                     QVSFRFYYTGFYNSIYKQVETRRFTTTPFEVEFNSGEKFIYRWTPSFERLFEPFRIHE
                     GVAIPAGAYWFHRHEFSFETADNRPLAFKIKTETGSFYSGKSHEVSPQLIWRKDHRLT
                     TSFELQQYWVNLREGEFRTRLALWRLSYSFGPRLTLANFVQYDTDSRNLGLQSRLRWI
                     IRPGNELFFVVNHAWQYNSMDRFEAITTNVRAKLNYTVRF"
     gene            complement(5125..5607)
                     /locus_tag="VFQ79_04670"
     CDS             complement(5125..5607)
                     /locus_tag="VFQ79_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0138975.1"
                     /translation="MTNEHEYRIATTWTGNLGTGTSGYRAYRRDHEIHAAQKSASIPG
                     SSDPIFRGDPSRYNPEELLVAALSACHMLSYLHLCAEAGITVTDYRDEAAGYMAMEKD
                     GGGRFTRVVLRPQVAITDTARAQEAISLHDKAHGLCFIARSVNFTVEHEASITEPRAG
                     "
     gene            complement(5597..6562)
                     /locus_tag="VFQ79_04675"
     CDS             complement(5597..6562)
                     /locus_tag="VFQ79_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0138976.1"
                     /translation="MDLMLSWLVVGIGDITQKRVLPAILAGTRCKLTGIVTRDPAKAA
                     PYGVRAWTSLEDALEESDAEAVYVATPVFLHAPQTIASLHAGRHVLCEKPMAMNYAEA
                     CSMERAAKDAGKLLGIAYYRRMYPKISRTVELLKAGAIGVPVLAEASSHDWFFPADRE
                     RDWFIDPRRAGGGPLYDIASHRIDLMNYLFGQPARASGHISTLIHPMDVEDNATVLID
                     YANGVRGVVDVRWHSRTPRDEFRIRGTEGEINLTPLNGPSLVYPGGEESIPPHGNLHY
                     PCIENFVGAVLDGAPLRSSAETALWTDWVTEQIMAAQEGLTSHDK"
     gene            6653..7033
                     /locus_tag="VFQ79_04680"
     CDS             6653..7033
                     /locus_tag="VFQ79_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0138977.1"
                     /translation="MALVNLSRRERQIMEILYQRGMASASEIREAMEDAPSYSAVRSI
                     LRVLENKGHIRHQAENFKYVYLPVVNREKAKRSAVKHVLGTFFEDSPDQMVAALLDVS
                     SKRLKREDLDRMAELIERARREGK"
     gene            7034..8974
                     /locus_tag="VFQ79_04685"
     CDS             7034..8974
                     /locus_tag="VFQ79_04685"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0138978.1"
                     /translation="MFGWNVSEWVPLLAGVAVKSAVVLGAAWMAALLLRKQSAAARHV
                     VWTTAFAALLALPLLSVSLPALRVPVAPQWLPPDLFFETTATLGTSEVPAVQPASTAV
                     NTGSAPWRPNWRLWLAFAWLGGAALSFSHMLVGWIAVQRMRRNARPFPAAIEGEIDIL
                     ETMPGSMPAACGLFRPVILLPADAREWSEERRKFVLLHEAAHVRRGDMATHFLARIAL
                     SLYWWNPLAWTAWREFLKERERAADDLVLNSGARASDYAAHLLDIARSMQSTAGVAAA
                     AVPMARVSQLEGRLLAILDAGRNRASSGRMSVVAAALLAAVFVLPVAAFQVQAPATEI
                     ADADLDAAIRAVASARNPERLEQAAQMAQRLRKYDIARKLLDSALSIRAETSGRQSPE
                     YATALLKLGDLERKRGRLSGAVPFYTEALSILGDRSETGSALIALGIQTLNPKKPDQA
                     IAYLERARVVSPAHAASALMWMAVAREQEANFPAAEMLYQQALAAAVPGTPETATIME
                     LYALFLGRQGRESEAANFRNQAGTLRRSFLEQAGTKSPGSGDNVYRIGGDVAPPKLLF
                     KLEPEYSEEARAAKYQGTAVISVEIGLDGKPGNLKIQRSLGLGLDEKAIEAVNQWKFE
                     PGTRNGQPVTVAATIEVNFRLL"
     gene            complement(8987..10756)
                     /locus_tag="VFQ79_04690"
     CDS             complement(8987..10756)
                     /locus_tag="VFQ79_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEU0138979.1"
                     /translation="MRGRAFAAAFCLIGLATVSHAGVRAIWAVNDGEKIERDDLSNPN
                     KARNSAWDGKTIRLFGARNEVIAFQVIVESDAKPVEAVSIALPKLSGGRSVIQYRPPA
                     ADPSNYAGRNIQLFSVNCMLVKEPSRANWVWRPDSPAAPKDPTGWKPVQIVPENARKG
                     RGGFPLCIEPESNQAFWVEIYTPRDLAAGTYRGEVAVQADGRRHRVPVQLELFDFTLP
                     DENSMHAMLYYSGDQPELYQGRNMDAEYHRFAHRNRVELVHAYNESSLKAAMSRFDGK
                     AFLAAEGYSGPGEGTGNRIVPLTFYGPGKQFDEKADAWKRSDAWMSFLAEAVPKALTF
                     LYLPDEPGRRQFEYIRRLGDNIHSNPGPGGKLPTFVTHSYTPELEGAIDYWCSGPRGY
                     RIEMALKERAQGRDYWIYNGGRPAGGAITIDAPATDPRSMIWASFKHGIGLYFYWYAT
                     HWRHNSQKQGERNQNVWADTVTFDNRGQPNKPIRDQGFIHGDGVLIYPGEDKLHPDQD
                     RGIAGPISSVQLANFRRGLQDHQYLTIAKQLGLDEVVRTSVEKIVPRVFSEAGETASF
                     SESGNDYEEARYKLAQAIAQAKR"
     gene            complement(10753..14001)
                     /locus_tag="VFQ79_04695"
     CDS             complement(10753..14001)
                     /locus_tag="VFQ79_04695"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HEU0138980.1"
                     /translation="MTLQRTAFRAATLLLAALPVFAQVKVWEGTLELPTYEEDLPNPN
                     PPFDLFATSRFSNYPYTIRDNLTNRRVTHAWRALYLENEYLKCSVLPDIGGHLYSCTD
                     KVNGQEIFYANPSIKKANIGYRGAWAAFGIEFNFPVSHNWMSMSPVDFASRQNADGSA
                     SVTVGNVDRVYGMQWRVELTLRPASMVLEQKVQLYNRSDLRHRFYWWNNAGVEVWNDS
                     RVFYPMRFTASHGFTDVDTWPVNRRGRDVSLIANQLDGPVSEFVHGSREPFMGVYHPR
                     TNAGTVHYADYAELPAKKIWSWGVNPDGLDWRKTLSDNNSAYVEVQGGLFRNQETYAF
                     LEPQKTLEFTEYWMPVRDIGGISRANLHGVMHFLRKESAAGKVTLTAGLNVNHAVRDA
                     RVRILDGTKTLFEQRLPLDPRRTFSHSVPDLPADRQYTFEVADADGHVLLAHTEGKYD
                     WTPADQIKTGPQKAYEFPPVDKRTEGDFVELGKDQELNGLLLRAYETYTSGLKRFPQS
                     FEINKAMGRLAVDLMRYKEAVERLRLAQQRVSNDDEIHYYLGQAFKALGQLRDARTEF
                     EGAQRLPALRGAANLELARLSARQNDLRKALEEISAALKAEPQSIRTGEAEVTLLRHL
                     DNASAARKRLDHWMKVDPTSGTLRYEHTKLGGQDEGLWSHLAGDPERVLAMADDYMAL
                     GFWSDALDVLSRKYPAVSPLQAEPGTPLPQDHPMVSYYAGYAREMLKQSGKADFQRAS
                     RLSTAYVFPSRASGLAVLGKALEVNPGDATARFLLGSIQFSNGQVEESIALWQQARKL
                     NPKIPVLHRNLGRTLLDVKGETTQGVDVLREGLKADPRNMELYLSLNRGLSLLRRPAT
                     ERTEVLRQYPDRKALPQSLLYELILSLAESGQAEEAEKLFHNRFFAREEGGINVRQVW
                     VEVQLRKALALARSGRGKEAVAVLDRAAKPVEGLAFTNDGMDVFTRGARFEYYSGVVR
                     EAAGDKDGARAAFQRAKSGGGPGYQLGFAALASRKLDGSVDSALKSRVDAALARSSGG
                     ARNASMTAYSRGVLLQAMGRGDEAESALLTALKAPDRLLAHYLTRIALEEKQ"
     gene            complement(14003..15223)
                     /locus_tag="VFQ79_04700"
     CDS             complement(14003..15223)
                     /locus_tag="VFQ79_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0138981.1"
                     /translation="MEQSNRREWLARLGTVAAGAAAVSTGCTQAPDSAKEATPTKKPL
                     DLSEFQPTSMLRVPEHKVERAKYPVIDIHTHLAWAAESKNGVSLTGNLSFIVPPEELL
                     VVMDRRNIRAMVNLTGGYGDGLRESVQKYDQAHPGRFYTFTEPMWTQVNQPGYPKQQA
                     DAIAQAAKDGARGLKILKTLGLYLRDNVTSGALVKVDDPRFDPMWEACGANNLPVAIH
                     VSDPAAFFLPTDRFNERFEELNNHPDWSFHGQDFPKNEELLEARNRVLARHPKTQFIV
                     LHVGNYAEDLGNVSEAMDRYPNMTVELGARIGELGRQPRTARKFFEKYQDRILFGTDA
                     VPKGDEYPQQVFGDLLYQIYYRFLETEDEYFDYAPAAVPPQGRWQIYGVGLPDRILEK
                     VYNANAARLLRIKV"
     gene            complement(15230..16165)
                     /locus_tag="VFQ79_04705"
     CDS             complement(15230..16165)
                     /locus_tag="VFQ79_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU0138982.1"
                     /translation="MKILVAGELNPDLILQGYEIFPTPGKEILVDDLNLTMGSASAIC
                     AMGLARLGNRVSFLAKVGKDPWGEFCTGILEEGGIDTSRVIRDANLKTGLTVSVSAPG
                     KDRALVTYLGAISELEGGDITDEVLSGFDHLHISSYFLQQKLRRDVRSVFALARRLGL
                     STSLDPGYDPSERWENDLIETLPEVHVFFPNEVELSAITGEEQPAEALRKLANGRTLT
                     VAKLGGNGCMAFDGGEIVEEPAIPVKPVDTTGAGDSFNAGFIHAWLRKFSLRDAMRFA
                     SVCGGLSTQGVGGTSAQATEGQVHTYLNSSTGSFR"
     gene            complement(16162..17343)
                     /locus_tag="VFQ79_04710"
     CDS             complement(16162..17343)
                     /locus_tag="VFQ79_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0138983.1"
                     /translation="MVTELRHLLEQPEAAQQEAGYLHTVREIHQQPQTWVETAKNVAE
                     LRPLLDKALSGIETICLTGSGSSEYAGDCIAPALRHDLGRPVQVIGGGTLLTHGASFL
                     SPKRPALMISLARSGNSPESAEAVRFLLETEPGVRHLMITCNAQGRLATAHTGRSDVF
                     VVTLDDRTNDRSLVMTSSFTNMVVAALFLGLLNDAPAYTKIAGKLSEAGRFILDNHTD
                     AIAGIARGGFRRAFYLASAPAYGAARESALKMTEMTGGRVMTVAETFLGLRHGPMSAI
                     HADTLVICYLSSDPLVRSYETDLIRELNRKSLGAAKLLVGENVPADLLHASDVVLEVP
                     GLGEIGDRYAPVLHVMVGQLLGLFRSMAEGLKPDAPSESGVINRVVESFTIHRLDGVS
                     R"
     gene            complement(17402..18991)
                     /locus_tag="VFQ79_04715"
     CDS             complement(17402..18991)
                     /locus_tag="VFQ79_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0138984.1"
                     /translation="MAEAIVYDGGVMKNRRQFLAGAVSATAALAQQPRNPSRPNILHI
                     MSDQQQWETIAGRSECRTPNIDRLAKSGMLFERSYTPSAVCCPARAILLSGAYHWHNG
                     VYNQIHSSPSVHRDMFPDVVLYSQRLRDAGYRLGYVGKWHSSWLRGPFEFGFQEVADL
                     TSYRGAAKDNERSNVKVQRSKDQLRRVVKRTMQWPGSEPFPMWGYADGPEEATPEYYR
                     TDCAIRVMQRLAAGNGPWHLEVHYIQPHDPYFPLKKYYDRYDPRSIPVPDNFRDDFKG
                     KPGLHRRESETWGKVSEDDVRASRAAYYAQAEQLDAQIGRLLDALDKTGQAENTMVVF
                     TTDHGDMVGGHRMWIKGWIPYEECYRVPMIVRWPGRIRPGSRTRRLVQTHDLAHTYVT
                     AAGAAAMPYADGRDLHPLFADPEGPGWADEILCAYYGGEFLYTQRMAITSRFKYVFNG
                     FDFDELYDLENDPGEIRNQVDNPQYRTDADQMRERIYALMTRFGDPYGGGAQPPGGVG
                     ERPNRYGAPRYLFLSRQNTTR"
     gene            complement(18958..>20248)
                     /locus_tag="VFQ79_04720"
     CDS             complement(18958..>20248)
                     /locus_tag="VFQ79_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0138985.1"
                     /translation="AGSLQPTANGLYDMIGNVWEWVYDYANPTSAVYSAAGSSGPGIG
                     VARIVRGGSYASTTSRWARGYRSSIEPDRKSRFTGFRVARNQPAPAAKPARPAWLSEV
                     LTPPAEIGDSTGKLSALVPEGDRLDTWNENRAELRKKWNGILGSMNLTPPTPAVRVVE
                     TFEEPAYSGRLMYLQVEPDYWEKILVMTPHTRVSKPLPVVIVPYYDVDTPAGKDLGGR
                     AFTPLGTRSFAHLAVQQGFIAVAIRWFGESYAEHYAEAVANLKLRHPDVTGLGKWVWD
                     SQRLLDYIYTLPDVDRSRIGIIGHSLGGKMALYASAMDDRITAVVSSEPGIGLSFSNY
                     EDYWYLGEKTRSLPAGTDHHELLAMIAPRPFLLIGGDDSDNAKSWYYINAAKAVYSLL
                     GKPDHIAYLNHHSGHSPTPDSVYKAMEWLKRSFMTEE"
BASE COUNT         3908 a         6271 c         5998 g         4071 t
ORIGIN      
        1 cccgaaaagc tgatcggctt ctgttcggtg gacccgcagg accctgacgc ggtcgaacag
       61 ctcgaccact gcgtgcagga tctggggctt cgcggcttga agatgggtcc catctaccag
      121 aatgttgccc cgacggattc acgatttcgc cgcatccttc accgggccga ggagttgcgg
      181 attcccacgc tcattcatca gggcaccacg ttttgcacga acgtgtcgct tgaaatcgcc
      241 aacccgattc agcttcagcc gctcgcgctg gagttcccac ggctcaagat ggtgatcgcg
      301 catctcgggc atccgtgggt gaacgaaaca ctcgtgctca tccggaagaa tcgtaacgtg
      361 ttcgccgata tttcggctct ttactaccgg ccctggcaat tctataacgc cctggtatcg
      421 gcgatggagt acggagtgct tgaccggcta ttgcttggct cggactaccc gttcactaca
      481 ccggcatcga ccatcgatgc cctgcgcgcg gtgaaccgga tggtggaagg cacccatttg
      541 ccgcgcattc ccgaagcgaa gatcgaagcg ttgatcgcgc gcgacacgct ggatctgctg
      601 ggactgtgag cgtcgcgcag agttcttacg cgatgcggta acgttcgatc gtatcttcgt
      661 tcacctccac gcccagcccg ggcgcttcgg gcacgcgcag gtagccgtct tcaagctgca
      721 gcggctcttt taccatctcg cggctcaatg ggccttgcga cgtgttgaac tcgacgaata
      781 cggcgcgccg ctggaacgca ttcagatgca gcgaagccgc cgtcagcagg tccgacgacc
      841 acgagtgcgg aatcaccagc acattcgcca tttccgccat gtgcacaatt ttgcgtgcca
      901 ccgtgaagcc gccgcagcgc gtaaggtcag gctgaagtat atcgacgcgg gcacgctcga
      961 tgagctcgcg gaagccccat tcggtggctt cctgttcgcc gcacgcgatg agagtttcca
     1021 cctccgcagc aacccggcga tagccgtcgt agtcttccgg gtgcagaatc tcttccacga
     1081 agaacggccg gtacggctcc aggctgcgta tcacttgaat ggcctgcttg ggcgttcgct
     1141 cgatgaacca gccggtgtcg acgagcagat cgtttcgatc gccgagctct gcgcgcgcgg
     1201 cttccaccaa ctccacatcg cggcgagggt cttcaccaaa cacgccccag ccgaatttca
     1261 ccgccgtgaa cccaaggtcc agataaccgc gcgcggcccg ccccatggct tccggcgtcg
     1321 gacggaatag cgtgctggcg taggccctca cccggttgcg ccacttgccg cccagcagaa
     1381 cacagacggg ctgtccgtag aacttgccca tgatgtccca cagcgcaata tcgatcgccg
     1441 atatcgcctg gatcgcgaca ccgcggcggc catagtaaat gctgccgcga tacatcttgt
     1501 accagagccg ctccacctcc agcggattct cgccaacgat gaggttcgcg agcccttcga
     1561 aacactcgac gcccgcttcg ctccaactcg gcgcttccac cgcggccttg gcaaccggtg
     1621 cggacgtctc catgtcggaa tagcctgtga tgccggcatc ggtcgagact ctgaccagtc
     1681 ccatgtaggt gggtccctgc ggctcctccg ctccttcggg gcggttgtag aggccgcgcg
     1741 acttcaagac gagcacttcg acgttcgtga ttttcatatc agagaatcac atcctgggcg
     1801 atgacaaaca gcgtgagacc tttgcggcac acggctggag aatactgcgc gatgatcact
     1861 ccgctgcggt gcgcggttac ctccagcggg tctgatgcgt gatcggaaaa gtcgtggatg
     1921 cgcccaatga gctgcccggc ttcgacatcg gctccgggct ggaacagcgg ctcccagact
     1981 ccgtcgcgcg ggcacggaat ataatctcgc agctcaggcg cgctcaccag gcggggcggt
     2041 gtgccccggg ccgtatcgat tggctcgact tcgccctcca gcaatccata atgccgcaag
     2101 acgtttttca ccccctcgta ggcgtgccgc acaccctttg gactggcgct ctccgcgaaa
     2161 ccgaattcgc cgccgatggc gatcttccct tcgtcttccg cttcgtccgt caacaagccg
     2221 gacgccatct gccgcgaata cacaaaaatg aacggcgggt cgaacagttt ggccacttcc
     2281 accatttccg cttgctgcgc gggatcgggc cgaggatgca gcgaagtgca gagcgggaat
     2341 acggcctcgt tgccgccgga gtgtatatcc agcacgatgc gaacacgagg aaacacacac
     2401 gtcttcacga aatgggcgat acggtaggag agcgtgcccc gagggtttcc cggaaacgcg
     2461 cggttcatgt tgacaccgtc gagcggggag atgcgcaagt tcgcccggca cgcgctctcg
     2521 ctcaattgtg gtatcaggat cgcacagccg cgcatccgct ccggatcgag gtcccggcag
     2581 agacgcttca ccgccacttg tccttcgtac tcgttgccgt gggtcccgcc gaagaccgcg
     2641 actcccgcag ggccatcacc gcgcaagccg tttatgaccg tcaccgggac cagcgaataa
     2701 ccccacgcgg agtccagatg gaacgccgcc tggtagtgat gcttaccggg caaggagaaa
     2761 tcaatttttg ccggatcgtg taggagcacc ttgcgattgt agtatcactc gcgcccgcat
     2821 acacacgagc ggaacagatt ctgtccaata aatgggaaac tgtggtgaag gcgcagccgc
     2881 tccgtgaccg tcgcggctca gaacaaaggt gtaacggagc cgcgcgcgtc agcaagcggc
     2941 tagaaccgca ccgtatagtt cagcttggcg cgcacgttgg tggtaatcgc ttcaaagcgg
     3001 tccatcgagt tgtattgcca ggcgtggttc acaacgaaaa acagctcgtt ccccggccgg
     3061 atgatccatc gcagccggct ttgcagaccc aggtttcgcg aatcggtgtc gtactggaca
     3121 aaattagcga gcgtcaacct ggggccaaac gaatagctca gccgccagag cgccagccgc
     3181 gtacgaaatt cgccctcccg taagttcacc caatactgct gcaattcgaa cgatgtcgtc
     3241 aggcggtggt ccttgcgcca gatgagttgc gggcttactt cgtgactctt cccggaataa
     3301 aaggaaccgg tttcggtttt gatcttgaac gcgagcggcc ggttgtccgc tgtctcaaaa
     3361 gagaactcat gccggtgaaa ccagtaggcg ccggccggga tcgcgacacc ctcgtgaatc
     3421 cggaagggct cgaacagcct ttcgaaagat ggcgtccatc ggtaaatgaa cttctcgccg
     3481 ctgttgaatt ccacttcgaa cggtgtggtg gtgaatcgcc gtgtctccac ctgcttgtag
     3541 atggagttgt agaagcccgt gtagtaaaag cggaagctca cctgccgcat gttccagaaa
     3601 tccggccgcg gacggaagtt gcttcccatg gccgattccc gcacacccgt gcggcgcatg
     3661 aacccgagag ccggattgta gttcttcccg atgaagcgcc accggtacca gacgtacaac
     3721 aggtcgttcg gatacaccac ttccccgccc caaaacatat cgtcaccctt ccggcccgta
     3781 gtggatgttt tcgatccgta gaagagcgag cggaaattct tcccggtttt gaacaggtcc
     3841 gccgtagcga tcttcaggtc cactccaacc tgccggtttt cgagcttgcc cgtcggctca
     3901 ccgtccgtca ccagcgcacc gatgtaggat tgcgaccaga agttcgcctt cgtccgcccg
     3961 acgaaaaagt tgcgtcccgg agcgacctcg gaatcgcgcg tcttcacgtc catcaggccg
     4021 acgtcgaagc ggccgatctt tccggtcacc ttctgaccga acagaatcgg cacttccaag
     4081 ccatccacca gtccgatgcg ccgggagaaa tagggcgtaa agtcgcgagt gccggggctc
     4141 gcggcaaatt cgaagatgcc tgaatcttcg aggaagaact ggcggcgttc cgggaacaac
     4201 accgggaacc gcgtgaggtt gatctggcgc gtgtccactt ccgtctcggc gaaatcggta
     4261 ttgatggacg tgctcgaaac caggttggcg gtgatgcggt agaagatgtc ggctccggcg
     4321 tcgcgtgcga actggtggcg gtcccgaagg ccgatatcgc ggctgtagcc acctagcgca
     4381 tacggcctca catccaagcc gatgccctgt gtcagcccct ccaatccatt aacgtcgccg
     4441 gcgcgggcca ccagaaaaaa ttcgctgtcg agattcgaag acgtccagcg cgactcctcg
     4501 cgcagccgcg cgattcgccg ggaaatattg aaaccccatg tgcgcgaacc gggattgaaa
     4561 gcgatcgttt tgaatgggat ttccatttcc acgctccacc cctcgctgtc gcgctgtggg
     4621 cgcacgtccc agatgccgtc ccagttcaga ttgggcgtat tgttttccga aatggtgcct
     4681 tccaccatca caccggcggg gttggtagcg aagtagtagg cattccggcg gtcggcatag
     4741 gtgtcgaata ggatctgcac gtagtcgtcg aacagcaggt cgccgtcgcg ctgcatctgc
     4801 gtcgcccgaa gttctccggg ccgcgagtcg tagcagcgga tagccacgta gatggcgtca
     4861 tcgttataag ccacgtggac ctcggtcgct tcagacggcc gcacgcccgg tacaggttct
     4921 ctctgcacca gctctccaat gccgccattt tcgcgccaga ctcgttccga aagaacgccg
     4981 tctacatgga ttcgctccga tatccgtacc gcaaccgtct gtctggttcc atttgcctca
     5041 ccgggttgcc cggccagtgc cgctcgaagg atgaaaacgc acactacgag cgggccgacg
     5101 cttcgcataa agcatcatgg taccctagcc ggcgcgcggc tcagttatcg acgcctcgtg
     5161 ttcaacagtg aagttcacgg accgggcaat aaagcacaat ccgtgtgcct tgtcgtgcaa
     5221 agaaatcgcc tcctgggcgc gagcggtgtc cgtaatggct acctgcggcc gcaaaaccac
     5281 gcgtgtgaac cggccgccgc cgtctttctc catggccatg tacccggcgg cttcatcgcg
     5341 gtagtctgtc accgtaatac cggcttccgc gcaaaggtgc aaatacgaaa gcatgtggca
     5401 ggccgatagt gcggccacca gcagctcttc gggattataa cgcgagggat cgccccggaa
     5461 tatcggatcg gaagagcccg gtatggatgc agacttctgc gcggcgtgaa tctcgtgatc
     5521 gcgccggtac gcgcggtagc cgctggttcc agtccccaga ttgccggtcc acgttgtggc
     5581 aatccggtat tcgtgttcat ttgtcatgtg aagtgagccc ttcctgtgct gccatgatct
     5641 gctcagtcac ccaatcggtc catagcgctg tttctgcgct ggagcgcagc ggcgcaccat
     5701 cgaggaccgc acccacaaaa ttctcgatgc agggatagtg aagattcccg tgcggcggaa
     5761 tcgactcttc gccgccggga tatacaagcg acggcccgtt cagcggcgtc aggtttattt
     5821 cgccttccgt tccacgaatg cggaattcgt cacgaggcgt gcgcgaatgc caccggacat
     5881 cgacaacgcc tcgaacacca ttcgcgtaat cgatgagcac agtggcgttg tcctccacgt
     5941 ccatggggtg aatcaatgtc gagatgtggc cgctggcccg cgccggctga ccgaaaagat
     6001 aattcatcag gtcgatgcgg tgcgaggcga tatcgtacag cgggccgccg cccgcgcgcc
     6061 gcgggtcgat gaaccagtcc cgctcccggt ccgctggaaa aaaccaatcg tgcgaagacg
     6121 cctcggcaag tacgggcacg ccgatcgcac ccgcttttag cagctcgaca gtccggctta
     6181 tcttcggata catgcgccgg tagtatgcga tgcccagcag ttttccagcg tccttcgctg
     6241 cccgttccat agagcaggct tcggcatagt tcatcgccat cggcttttcg cataaaacat
     6301 gcctacccgc atgcaaagaa gcgatagtct gcggggcgtg caggaacacg ggtgtggcaa
     6361 cgtaaaccgc ctctgcatcg gattcctcga gcgcgtcctc aagactggtc cacgccctca
     6421 ctccgtacgg cgcagcctta gccggatcgc gcgtcacaat gccggtgagt ttgcagcgcg
     6481 tcccggcaag aatcgccggg agaacgcgct tttgcgtgat gtctccaata ccaaccacca
     6541 gccagctgag cataagatcc atggtagccg ttcaaaaaac ggttgacatc gggatcggta
     6601 ggtgctaatt tttgagcatg gtgctgaaat ttaagcatag aggacaccgc ctgtggcact
     6661 cgtaaacctg agccggcgcg aacggcagat catggagatt ctttaccagc gtggtatggc
     6721 atccgcttcc gagattcgcg aagccatgga ggatgctccc agctattcgg ccgtgcgttc
     6781 gattctgcgc gtgctggaga acaagggaca catccggcac caggcggaaa acttcaagta
     6841 tgtatacctt ccggtggtga accgggagaa agcaaagcgc tcagccgtga agcacgtact
     6901 gggcactttt ttcgaggact cgccggatca gatggtcgcc gcgcttctgg atgtttcttc
     6961 caagcgcctg aagcgggaag acctggatcg tatggcggaa ttgattgaaa gagccagacg
     7021 ggagggcaaa taaatgttcg gctggaacgt atccgaatgg gttccgctct tggcaggcgt
     7081 ggcggtcaag agcgcggtgg tgttgggtgc ggcctggatg gccgcgttgc tgttgcgaaa
     7141 acagtctgcc gccgccaggc acgtagtgtg gaccaccgcg ttcgccgccc tgctggcgtt
     7201 gcctctcctt tccgtctcgt tgccggcgtt gcgcgtgccg gttgcgccac aatggctgcc
     7261 gccggactta tttttcgaga ccacggcgac gctaggaacc agtgaggtgc cggccgtcca
     7321 acctgcaagc acggccgtca ataccggttc cgcaccatgg cgccccaact ggcggttgtg
     7381 gctggcgttt gcctggctgg ggggagcggc gttgagtttt tcccacatgc tggtgggatg
     7441 gatcgccgtt caaagaatgc gccgcaacgc acgtccgttc ccggccgcca ttgaagggga
     7501 aattgacata ctcgaaacca tgccgggcag tatgcctgcg gcgtgcggcc tgtttcgtcc
     7561 ggttattctc ctgccggcgg atgcaaggga atggagcgag gagcggcgca agtttgttct
     7621 gctgcatgag gcggcccatg tgcgccgcgg cgacatggcg acacactttc tggcgcgcat
     7681 agcgttgagt ctctattggt ggaacccgct ggcctggacc gcctggcgtg aattcctgaa
     7741 agagcgcgaa cgcgcggccg acgatcttgt tctgaactcc ggcgcgcgcg catcggacta
     7801 cgccgcccac ctgctcgaca tcgcgcggtc gatgcaatcc accgccggtg tggctgctgc
     7861 cgccgtaccc atggcgcgag tttcccaact ggagggacgc ctgctggcga tactggatgc
     7921 aggacgcaac cgtgcctctt ccggccggat gtcggtggtt gccgctgcgt tgctcgcggc
     7981 cgttttcgtg ctgccggttg ccgcctttca agtacaggcg ccggccacgg agatcgccga
     8041 cgcagacctg gacgccgcca ttcgcgcagt cgcatccgcg agaaaccccg aacggctcga
     8101 gcaggccgcc cagatggctc aaagactccg caaatacgat atcgcccgaa agctgctcga
     8161 ttccgcactg tcgattcggg cagaaacttc cggcaggcag agtccggaat acgcgacggc
     8221 cttgttgaag ctaggcgatc tggagcgtaa gcgcggccgc ctttccggcg cggtgccttt
     8281 ctacacggag gccctctcga tcctcggaga tcggtccgaa accggttcag ccctcatcgc
     8341 tctcggcatc cagacactga atccgaaaaa gccggaccag gccatcgctt atctcgaacg
     8401 ggcacgggtg gtatcgccgg ctcatgccgc ttccgcgctg atgtggatgg ccgtcgcgcg
     8461 cgagcaggaa gcgaacttcc ctgcggcgga aatgctctat cagcaggcct tggcggctgc
     8521 ggttcccggc acgccggaaa cggccaccat catggagctg tatgcgctat tcctcggccg
     8581 gcagggccgc gagagcgagg cggccaactt ccggaaccag gccggcacac ttcgaaggtc
     8641 cttcctcgaa caggccggca caaaatctcc cggctccgga gacaacgtat atcgcatcgg
     8701 aggagacgtc gcgcctccca agctgctgtt caaattggag ccggagtata gcgaggaagc
     8761 tcgcgccgcg aagtatcagg gaacagcagt catctccgtc gaaatcggcc tcgatggaaa
     8821 acctggcaat ttaaagatcc aacgcagtct cggactcggc ctggacgaaa aggcgatcga
     8881 agccgtcaac caatggaagt tcgagcccgg caccagaaac gggcagccgg tcaccgtagc
     8941 ggcgaccatc gaagtgaact tccgcctcct ttaagggggg cggaagttat ctcttcgcct
     9001 gtgcgattgc ctgcgccagc ttgtagcggg cttcttcgta gtcgttgccg gattccgaaa
     9061 aggatgccgt ctcaccggct tcggaaaaca cgcgcggtac gatcttttct accgatgtcc
     9121 ggaccacctc gtccaacccg agttgcttcg ctatcgtcag atactggtgg tcctgcaagc
     9181 cgcggcggaa gttggccaac tgcaccgaag agatgggacc ggcaatgccc cggtcctgat
     9241 ccgggtggag cttgtcttca cccggataga tcaacacgcc gtcgccgtga atgaagccct
     9301 ggtcccgtat cggtttgttc ggctgcccgc gattgtcgaa ggtcaccgta tccgcccaca
     9361 cgttctggtt ccgttcaccc tgcttctggc tattgtgccg ccagtgggtg gcgtaccagt
     9421 agaaatagag cccgatgcca tgtttgaaag acgcccatat cattgagcga gggtcggtag
     9481 cgggcgcatc aatggtgatc gctccacccg cgggccggcc gccgttgtat atccagtagt
     9541 cgcgtccctg ggcgcgctct ttgagcgcca tctcgatccg gtagcctctg ggtccggagc
     9601 accaatagtc gatagcgcct tcgagctccg gcgtgtagga gtgcgtgacg aaggtcggca
     9661 gtttgcctcc gggacccgga ttgctgtgta tgttgtcccc tagccgccgg atgtattcga
     9721 actgccggcg gcccggctcg tccggcagat aaaggaatgt cagcgccttc ggaacggcct
     9781 ccgccagaaa gctcatccac gcgtccgatc gtttccaggc atcggctttt tcatcgaact
     9841 gcttgccggg gccgtaaaac gttagcggga caatccggtt accggttccc tcacccggac
     9901 cagagtatcc ctccgcggcg aggaacgctt tgccgtcgaa acggctcatg gccgctttca
     9961 aggaggattc attgtaagca tgcaccaact ccacccggtt gcggtgtgcg aagcgatggt
    10021 actccgcatc catgttccgg ccctgataca actcgggctg atcgcccgaa tagtacagca
    10081 tcgcgtgcat gctgttctca tccggcaatg tgaagtcgaa cagctcgagc tgcaccggaa
    10141 cgcgatggcg ccggccgtcc gcctgaaccg caacttcgcc gcgatacgtg cctgccgcaa
    10201 gatcgcgcgg cgtgtagatc tcaacccaga acgcctggtt cgactcgggc tcgatgcaca
    10261 gcggaaaacc gccccgcccc ttgcgtgcat tctcgggaac gatctgtacg ggtttccatc
    10321 cggtcggatc tttcggcgcc gcgggcgagt ccggacgcca gacccagttg gcccgcgaag
    10381 gctccttcac cagcatgcag ttgacggaga acaactggat attgcgccct gcgtaatttg
    10441 acgggtcggc cgcgggcgga cggtactgta tcaccgaacg cccgccagag agctttggca
    10501 gcgcgatcga aaccgcctcc accggcttcg catccgactc gacaatcacc tgaaaagcga
    10561 ttacttcgtt gcgcgcgccg aacaggcgga tggtcttgcc gtcccacgcg gaattgcgcg
    10621 ccttgttcgg attcgacaga tcgtcccgct caatcttctc gccgtcgttt accgcccaga
    10681 tggcacgaac gccggcgtgg gaaaccgttg ccaggccaat cagacagaat gctgccgcaa
    10741 acgcgcggcc tctcactgct tctcctcgag cgcaatacgc gtgagatagt gtgccaacag
    10801 gcggtccggc gctttcagcg ccgttagaag cgcggattcg gcttcgtcgc cgcggcccat
    10861 ggcttggagc agtacaccgc gcgaatacgc agtcatcgac gcgttgcgcg cgccgccgga
    10921 actgcgcgcc agagcggcat cgacgcgcga cttcagcgcg gaatccacac tgccatccag
    10981 cttgcgcgaa gcgagcgccg cgaaaccgag ctgatagcca gggccgccgc cggacttggc
    11041 gcgctggaat gccgcccgcg ctccgtcctt gtcgccggcc gcctcccgaa ccacgcccga
    11101 gtaatattcg aagcgcgctc ctcgcgtgaa aacatccatg ccgtcattag tgaacgccaa
    11161 tccttcaacc ggcttggcgg cgcggtccag caccgctaca gcctctttgc cacggcccga
    11221 gcgcgccagg gccagcgcct ttcgcaactg gacctccacc caaacctgcc gcacgttgat
    11281 gccgccctct tcccgcgcaa agaagcgatt gtggaaaagc ttctccgctt cttccgcctg
    11341 gccggattcg gcaagactca ggatcaactc gtataacagc gattgcggca gagccttccg
    11401 gtcgggatac tgccgcagca cttccgtgcg ttcggttgcg gggcggcgca gcaggctgag
    11461 gccgcggttc aggctcaaat acagctccat gttacgcggg tcggctttca acccctcgcg
    11521 aagcacgtct actccttgcg tagtctcacc tttcacatcc agcagcgttc gccccaggtt
    11581 gcggtgcagc accggaatct tcggattcaa cttccgtgcc tgttgccaga gggcgatgga
    11641 ctcctccacc tggccgtttg aaaactgaat cgaacccaac agaaagcgcg ccgtcgcatc
    11701 gcctggattt acttccagtg ccttgccgag caccgccaga ccggaagcac ggcttggaaa
    11761 aacataagcc gtcgacagcc gggaggcgcg ctgaaaatcc gccttcccgc tttgcttcag
    11821 catctctctg gcgtaaccgg cgtagtagga aaccatggga tggtcctgcg gaagaggtgt
    11881 tcccggctcc gcttgaagcg ggctgacggc cggatacttg cggctcagaa catcgagggc
    11941 atcgctccaa aaaccgagcg ccatgtaatc gtcggccatg gccagcacgc gctcgggatc
    12001 gcccgccaaa tggctccaca atccctcatc ttgcccgccc agcttggtgt gctcgtagcg
    12061 cagagtgccg ctcgtcgggt ccaccttcat ccagtgatcc agccgcttgc gcgccgccga
    12121 ggcattgtcc agatgccgca agagcgtcac ctcggcttcg cccgtgcgaa tggattgcgg
    12181 ttcggccttt agcgccgccg atatctcttc caacgccttt cgcaaatcgt tctgccgcgc
    12241 cgaaagccgg gccaattcca gattcgccgc gccacggagc gcgggcagac gctgcgcacc
    12301 ttcgaactcc gtgcgcgcat cccgcagttg cccgagcgct ttgaacgcct gtcctaagta
    12361 atagtgaatc tcatcgtcgt tgctgacgcg ctgctgggcc aggcgcaaac gctccacggc
    12421 ttccttgtaa cgcatcagat ctactgcgag ccggcccatc gctttgttga tttcgaagct
    12481 ctgcggaaag cgcttcagac cgctggtgta agtctcgtat gcgcgaagca gcaggccgtt
    12541 cagctcctgg tctttgccga gctcgacgaa atcgccttcg gtgcgcttgt caacgggtgg
    12601 aaactcgtac gctttctgcg gccctgtctt gatctggtct gccggtgtcc agtcgtactt
    12661 gccttcggtg tgtgccagca gcacgtgccc gtcggcgtcc gccacctcga acgtgtattg
    12721 ccggtccgcc ggaaggtctg gaacagagtg cgaaaacgtg cggcgcggat cgagcggaag
    12781 gcgttgttca aacagagtct tcgttccatc caggatgcgc acacgcgcgt cgcgaaccgc
    12841 gtggttcaca ttcagtcccg ccgtcagcgt tactttcccg gcggccgatt ctttccgcag
    12901 gaagtgcatt acgccgtgga ggtttgcgcg ggagatgccg ccgatgtcgc gaacgggcat
    12961 ccagtattcc gtaaactcca gcgttttttg cggctccagg aaggcgtatg tttcctggtt
    13021 gcggaacagc cccccctgca cttcgacata agcgctgttg ttgtcggaaa gcgtcttgcg
    13081 ccagtcgagc ccgtccgggt taacgcccca cgaccagatc ttcttcgcgg gcaactcggc
    13141 ataatcggcg taatgaacgg ttccggcgtt cgttcgcgga tgatagacac ccatgaaagg
    13201 ttcgcggcta ccatgcacga attccgatac cggtccgtcc aactggttgg cgatcaggct
    13261 tacatcgcgg ccccggcgat tcaccggcca cgtatccacg tccgtgaagc catggctggc
    13321 cgtgaatcgc atgggataga agacgcgcga atcgttccag acctcaacgc ccgcgttgtt
    13381 ccaccagtaa aagcgatggc gaaggtcgga gcggttgtag agttgcacct tctgctccag
    13441 caccatgctg gcggggcgca gcgtcagttc cacgcgccac tgcattccgt aaacacggtc
    13501 gacgttgccg accgttaccg aagcgcttcc gtcggcgttt tgacggctcg cgaagtccac
    13561 cggagacatc gacatccagt tgtgcgaaac ggggaaattg aattcgatgc cgaaggccgc
    13621 ccacgcaccg cgatacccga tattcgcttt cttgatggag ggattcgcgt agaagatctc
    13681 ctgcccgttc accttgtcgg tgcagctata gagatgacct ccgatgtccg gtaacacgga
    13741 gcatttcaga tattcatttt cgagatacag tgcccgccag gcgtgcgtta cgcgccggtt
    13801 cgtcaggttg tcacgaatcg tataaggata gttagaaaag cgtgaagtgg cgaaaagatc
    13861 gaacggcggg ttcgggttgg gaaggtcttc ttcataagta ggcagctcga gagtgccttc
    13921 ccaaaccttt acctgtgcaa aaacgggtag cgcggccagc aggagcgtgg ccgcgcgaaa
    13981 agcagttctc tgaagcgtca tgtcaaacct tgattcttag caagcgggcg gcattcgcgt
    14041 tgtacacctt ctcgagaatg cggtccggca gccctactcc gtaaatctgc cagcgccctt
    14101 gcggcggcac cgctgccggc gcatagtcga agtattcgtc ttcggtttcg aggaaccggt
    14161 aatagatctg gtagagcaga tcgccgaaga cctgctgcgg gtattcgtcg cccttgggaa
    14221 cggcatcggt gccgaacagg atgcggtcct ggtatttctc gaagaacttg cgcgcagttc
    14281 gcggctgccg gccgagttcg ccgatgcgtg caccgagttc cacggtcatg ttggggtagc
    14341 ggtccatcgc ttccgagaca tttcccagat cctcggcgta gttgccgacg tgcagaacga
    14401 tgaactgcgt cttgggatgc cgcgcgagca ctctgttgcg tgcttcgaga agctcttcgt
    14461 ttttcgggaa atcctgaccg tggaaggacc agtccggatg gttgttgagt tcttcaaagc
    14521 gctcgttaaa acggtccgtc ggcagaaaga acgccgccgg gtccgatacg tgaatggcca
    14581 ccggcaggtt gttcgcgccg caagcctccc acatcggatc gaaacgcggg tcgtccacct
    14641 tcaccagcgc gccggaagtg acgttgtccc gaagatagag tccgagcgtc tttagaattt
    14701 tcagcccgcg ggcgccgtcc ttcgccgcct gtgcgatggc gtctgcctgc tgctttgggt
    14761 aaccgggctg gttcacctgc gtccacatcg gttcggtgaa cgtgtaaaaa cggcccgggt
    14821 gcgcttgatc gtacttttgc acggactcgc gcaagccgtc gccatagccg ccggtgaggt
    14881 tcaccatggc ccgtatattc cggcgatcca tcacgaccag caactcctcc ggcggcacaa
    14941 tgaaggagag attgccagtt agggaaacgc cgtttttcga ttctgcggcc catgcaagat
    15001 gcgtgtggat atcgatgacc ggatacttcg cccgttccac cttgtgctca ggcacgcgca
    15061 acatgctggt aggctgaaac tcgctcaggt caagcggttt cttcgttggg gtcgcctcct
    15121 ttgcggagtc gggggcttgt gtgcagccgg tgcttaccgc cgcggccccg gcagcgactg
    15181 ttcccaaccg ggccagccat tctcgtctat tggattgttc catgaaagct cacctgaatg
    15241 atcctgtgga tgaattgaga tacgtgtgga cctgtccttc ggtggcttga gcggacgtgc
    15301 cgcctacacc ctgcgtcgaa agaccgccgc aaacggacgc aaaccgcatc gcgtcccgta
    15361 gagaaaactt cctaagccag gcatgaatga agcctgcgtt gaaagaatcg cccgccccgg
    15421 ttgtatctac cggtttcacc gggatcgccg gttcttcgac gatctcgccg ccgtcgaaag
    15481 ccatgcagcc gttgccgccg agctttgcca cggtcagcgt gcgcccgttt gccagtttgc
    15541 gaagtgcttc ggcgggttgt tcttcgcctg taatggcgct caactcaact tcattcggaa
    15601 aaaagacgtg tacttccggc agcgtctcga tcaagtcatt ctcccaacgt tcggagggat
    15661 cgtaacccgg atcgagcgat gtcgaaagtc ccagccgccg cgccagggcg aacacgctgc
    15721 gcacgtcccg ccgcagcttt tgctggagga agtacgacga gatgtgcagg tgatcgaagc
    15781 cactcaggac ctcgtccgta atatcgccgc cttcgagctc ggagatagcg cctagatacg
    15841 tcacgagcgc ccggtctttg ccgggagccg ataccgaaac ggtcagtccg gtcttcaggt
    15901 tcgcatcgcg aatcacccgg cttgtgtcga tgccaccttc ctccagaatg ccggtgcaaa
    15961 actcgcccca gggatctttg ccaaccttgg cgagaaagga aacgcggttg cccaaccgcg
    16021 ccagccccat cgcgcagatc gcggacgcgc ttcccatggt cagattcaga tcgtcgacaa
    16081 gaatctcctt accaggcgtg gggaaaatct catagccttg caggatcaga tcggggttca
    16141 actcacccgc aaccagaatc ttcatcggga aactccgtcg aggcggtgta tggtaaagct
    16201 ctctaccacg cgattgatga cgccgctctc ggacggtgcg tccggcttga gcccttccgc
    16261 catcgagcgg aacaggccca gcaactgtcc caccatcacg tgcagcaccg gcgcatagcg
    16321 gtcgccgatc tcacccaggc caggcacttc cagaaccaca tccgaggcat gcagcagatc
    16381 cgccggcaca ttctcgccca caaggagttt cgccgcgccg agcgatttgc ggttcaactc
    16441 gcggatcaga tccgtttcat acgaacgcac cagcgggtcg gaggacaaat agcagataac
    16501 cagcgtatcc gcgtggatgg ccgacatcgg tccatgacgc aaaccaagaa acgtctcggc
    16561 gacagtcatg acgcgtccac cggtcatttc ggtcatcttc agagctgatt cgcgcgcggc
    16621 gccgtaggcg ggcgcgctgg caagatagaa ggcacggcgg aagccgccgc gcgcaatgcc
    16681 cgcgatcgca tccgtatggt tgtcgagaat aaaccggccc gcttcgctca gcttcccagc
    16741 gatttttgtg taggcggggg cgtcgttcag caagcccagg aacagcgccg caaccaccat
    16801 gttcgtgaaa ctgctggtca tcaccaggct acggtcgttc gtgcggtcgt cgagcgtcac
    16861 gacaaacaca tcggagcggc cggtgtgtgc cgttgcgagc cgcccctggg cgttgcaggt
    16921 aatcatgagg tgccgcacgc cgggttcggt ttcaagcagg aatcgcaccg cttccgcgct
    16981 ttccgggctg ttgccggagc gcgcgagtga aatcatcagc gcgggacgtt tcggggaaag
    17041 gaaggatgca ccgtgcgtca gcagtgtccc tccgccaatc acctgcacgg gccgccccag
    17101 atcgtggcgc agcgccgggg cgatgcagtc gccggcatat tcagaacttc cggaaccggt
    17161 cagacaaatg gtctcgatgc cggacaacgc cttgtcgagc agcggccgca gctccgcaac
    17221 gttcttcgcc gtctccaccc aggtttgcgg ttgctggtgg atctcccgca cagtatgcaa
    17281 atagcctgct tcctgctggg cagcctcggg ctgttccagc aggtggcgca gttccgtcac
    17341 catgaatcca ttccttcaaa aaaagtataa caatacataa gtctttcggt ttcttttctc
    17401 tttatctggt tgtgttttgc ctggaaagaa aaaggtaccg cggcgcgccg taccggttcg
    17461 gccgttcccc aaccccgccg gggggctgag cccctccgcc atagggatct ccgaagcgtg
    17521 tcatcaatgc atagatccgc tcgcgcatct gatcggcatc ggtacggtat tgcggattgt
    17581 ccacctggtt ccggatctcg cccggatcgt tctcgagatc gtagagctcg tcaaaatcga
    17641 atccgttgaa gacatacttg aagcggctgg taatggccat cctttgtgtg tatagaaact
    17701 cgcctccata gtaggcacag aggatctcat ccgcccagcc gggtccctcg ggatcggcga
    17761 acagcgggtg aagatcgcgc ccatcggcgt acggcatcgc ggctgcgcct gcggcggtga
    17821 cataggtgtg cgccagatcg tgagtttgca ccaggcggcg ggtgcggctg ccgggccgga
    17881 tacgtcccgg ccatcgcacg atcatcggca cgcgatagca ttcttcatac ggaatccagc
    17941 cttttatcca catgcgatgc ccgccgacca tgtcgccatg gtcggttgta aacacgacca
    18001 tcgtgttttc cgcctggccg gtcttatcga gcgcatccaa cagccggccg atttgagcgt
    18061 cgagttgctc ggcctgcgcg tagtaagcgg cccggctggc gcgcacatcg tcctcggaaa
    18121 ccttgcccca tgtttccgat tcgcgccggt gcaatccagg cttgcctttg aagtcgtcgc
    18181 ggaaattatc gggcacggga atggagcgcg ggtcgtaccg gtcgtagtat ttcttcaagg
    18241 ggaaataggg gtcgtgcggt tggatgtagt gaacctccag gtgccacgga ccgttgcctg
    18301 ccgccaggcg ttgcatcacg cgaatggcgc aatcggtgcg gtaatactcc ggggtggcct
    18361 cttcgggacc gtccgcgtac ccccacatcg gaaacggctc cgaaccgggc cactgcatgg
    18421 ttcgttttac aacgcgccgt aattggtcct tcgagcgttg caccttcacg ttgctgcgct
    18481 cgttgtcctt cgccgcgccg cggtaggaag tgagatcggc cacttcctga aaaccgaatt
    18541 cgaacggccc gcgaagccag gacgagtgcc atttcccgac gtatcccagc cggtatccgg
    18601 catcgcgcag gcgttgcgaa tacagcacga cgtccgggaa catgtcgcga tgaactgaag
    18661 gcgacgaatg tatctggttg tacacgccgt tgtgccagtg ataggcgccg gaaagaagga
    18721 tggcgcgcgc cggacagcac acggcggaag gtgtatagga tcgttcaaac agcatgccgg
    18781 actttgccag ccggtcgatg tttggggtcc ggcactccga tcttccggca atcgtctccc
    18841 attgctgctg atcggacata atgtgcagaa tattgggccg ggacgggttt cgcggctgct
    18901 gcgccagcgc cgccgttgcc gaaacggctc cggcgaggaa ctgcctccgg ttcttcatca
    18961 ctcctccgtc ataaacgatc gcttcagcca ctccatggcc ttgtaaacgg aatccggtgt
    19021 cgggctatgg cccgaatgat ggttgaggta ggcgatgtga tcgggtttgc cgagtaagct
    19081 atagaccgcc ttcgccgcat tgatgtaata ccagcttttc gcgttgtcgg agtcgtcgcc
    19141 gccgatcagc aggaatggcc gcggcgcaat catcgcgagc agttcgtgat ggtccgtgcc
    19201 ggccggcagc gagcgggtct tctcgccgag ataccagtag tcttcatagt tggagaaact
    19261 gaggccgatg cccggttccg acgacacgac ggcggtaata cgatcgtcca tggcgctggc
    19321 atagagcgcc atcttgccgc ccagcgaatg gccgataatg ccgatgcggc tgcggtccac
    19381 atccggcagc gtgtagatat aatcgagcag ccgctgcgaa tcccaaaccc actttccaag
    19441 gccggtgacg tccgggtgcc gcagtttgag attggcgacc gcttccgcat agtgctcggc
    19501 atagctttcg ccaaaccagc gtatggcgac ggcgatgaag ccctgctgca cggcgagatg
    19561 ggcgaaggag cgggtgccga gcggcgtaaa cgcccgtccc cccaggtctt tgcccgccgg
    19621 cgtgtcgacg tcgtagtacg gcacaatcac caccggcagc ggcttggaca cgcgggtatg
    19681 cggagtcatg acgagaatct tttcccaata gtccggctcc acttgcaaat acatcagccg
    19741 gccggaatag gcaggctctt cgaacgtttc cactacgcgc acagcgggcg ttggtggtgt
    19801 cagattcatg ctgcccagaa tcccattcca cttctttctg agctccgccc ggttttcgtt
    19861 ccaagtgtcg aggcggtcgc cttcgggaac caatgcagag agcttgcctg tcgaatcgcc
    19921 gatttccgcc ggtggagtca gcacctcgct taaccaggcg ggccgagcgg gcttcgccgc
    19981 gggagcgggt tgattgcggg cgacgcggaa tcccgtgaag cggctcttgc gatcaggctc
    20041 gatcgatgag cggtatccac gggcccagcg ggaagtcgtg ctggcatagg agcccccgcg
    20101 gacgattcga gcgacgccga tccccggccc cgaagagccg gccgccgagt acacggcgga
    20161 cgtggggttt gcgtagtcgt agacccattc ccagacgttg ccgatcatgt cgtagagtcc
    20221 attcgcggtg ggttgaaggc tgccggca
//
LOCUS       DASTMK010000116        24951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_6974, whole genome shotgun sequence.
ACCESSION   DASTMK010000116 DASTMK010000000
VERSION     DASTMK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24951
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6974"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VFQ79_04725"
     CDS             <1..1536
                     /locus_tag="VFQ79_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138986.1"
                     /translation="KFNDYYDFFLNTFATPGRRPEAAGIRAGAVNTLGEVPDSAWYTN
                     RHGRKRMSIEELVKGPGISHEPSEKKPWKVTGAKLEGVSPGFAMEDAEGRKYLVKLDP
                     ESNPEMATGADVIGSKFFYALGYYTPENYIVTFRKEDLTIKKGTLIEDTRGYERPMRS
                     RDIDELLLHAAQYPDGTYRAVASRFIAGEIIGPFRFNGVRKDDPNDIVAHENRRDLRG
                     LYVFSAWLGHNDVKSLNTLDSIVEEGGLRYIRHYLIDFGAAFGSDSFEAKSPRAGHRY
                     LFAWDSAAAQVFSLGLYVPAWMRANYPKIPGVGNFEYEVVDALEWKPNYPNPAFLNRL
                     PDDEFWAARQVMQFTDEQINAIVKSGQYSDPRAVDWLTRCLIERRDKIGRAYFAKVLP
                     LDEFRVENGMLKFRDLGVDYGFTQSRRYRIRWSRFDNSAARESPIPSATTFELPRQFG
                     QARSPDFAVARIQADDRRQTVLVYLRFTDGRAEIVGIDRQWNSENGGSGRPDFARGNY
                     STK"
     gene            1484..3082
                     /locus_tag="VFQ79_04730"
     CDS             1484..3082
                     /locus_tag="VFQ79_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138987.1"
                     /translation="MAAVAALTLLAATTLQSEKFYPDDPLWTVPKPRNVEKLSKRKLN
                     DYYDFFLNTFGRPGSRHLPGEPVRALSANTLGEIPDSEWYTNRHAKKRMSLEELARGP
                     NTGTGPSESGDWTIISAKNQGITPGFLIKDAAGARFFIKFDPVENSEMATAADVIGSK
                     FFYALGYYVPQNYIVHFPRSRLRLEPHTTYRDSFGKRREMHERDVDEILRRVPRDEDG
                     RYRAVASLFLEGEVVGEFRYHGTRRDDPNDIIPHEHRRELRGLFVFAAWLGHDDSRAI
                     NTLDTLVEADGTRFVRHHLIDFGSILGSASTSSNSARSGHQHLFEFKPSVVEVLSLGL
                     WVPDWARVRYPDLPSIGRFDCSIFDPERFKTEYPNPAFENRLPDDTYWAAKVVMSFTP
                     DDIKAIVKTGAYSDPEAERWIVDCLLVRRERIGRTYFAKVVPLENLAIREGRLFFDDL
                     AVQYGFSKPREYDIRWFSLDNRSQQNTDVPGSGPAWPAELRDPAATPYFGAVIRVPNQ
                     HAALTAYFRHTPQGPQLVGVERTW"
     gene            complement(3093..3821)
                     /locus_tag="VFQ79_04735"
     CDS             complement(3093..3821)
                     /locus_tag="VFQ79_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0138988.1"
                     /translation="MKRSALLITLFGAALWIAGANRMTGQAVPAFQDLFNGKDLTGWV
                     NVNTDPETWSVKDGILVCKGRPIGVMRSEKQYENFILHVEWRHMEPGGNSGVFVWSNA
                     VPGEKNRLPNGVEVQMLDPEWVNLHKIKGETPPLAYVHGELFGVGGVETVPDNPRGPR
                     SKSIENRVQGNKEWNTYDVVAVDGVIKLAVNGKFVNGITRSTQKKGYLCLESEGAEIH
                     FRNIKLLELPPGVTTPEQTAPELD"
     gene            3893..4993
                     /locus_tag="VFQ79_04740"
     CDS             3893..4993
                     /locus_tag="VFQ79_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU0138989.1"
                     /translation="MHRYLGLFFLLATAFGQTSGVTWMAVPDARFEIDGLPWLSENRG
                     ELFRLPVRLKDNLPKAVWGLGESPSGGRIRFRTDSNKLLIRLEYPRAPGMRNMHAFGQ
                     TGVDLYLDGAYRGTAIATPEAKTGIPLEHTYFDFEDRPRVEREVTLYLPLYMPVKVLG
                     IGVDGKAGFSAPRPFALPKPVVFYGTSITQGGCASRSGLSYQAILGRMLNLDFVNLGF
                     SGNGKGEPEVARMVAEIEASCFVLDFAQNNGTVESLAAVYEPFIGTLREKYPEVPILA
                     VTPISNAREALAQDSRNEGMRRHIRQVVSRRIAQGDTNLQLMEGTDLLGPDRLDGLVD
                     GTHPNDLGFQWMAEGLAARLGKLLGLASASNR"
     gene            5047..5961
                     /locus_tag="VFQ79_04745"
     CDS             5047..5961
                     /locus_tag="VFQ79_04745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU0138990.1"
                     /translation="MKRRMLGLLAGLLVLSFTLAAAPFTTISVFGDSLSDNGNTFGVA
                     DYPPPPYYEGRFSNGPVAVEIFAGITGANLENHAWGGATTGSGNNVDGGNVSTAVALP
                     GMRTVFDATDLQPVSPETLFIVWGGPNDFVAPAPEDAGNPGAIAARAVGNLVYIAQGL
                     QAAGASNIWVLGLPDLGLTPFVRSQGAALSMAASFLTDQFNAALAAQISLLPNARFID
                     IAGTQRAIIGNPAAYGFSNVTDACLTSMGVCSDPGSYFYWDDFHPTTAAHSLLASDLA
                     AVVVPEPGTLVLLAGGLAALAALRRTRR"
     gene            5990..7180
                     /gene="lhgO"
                     /locus_tag="VFQ79_04750"
     CDS             5990..7180
                     /gene="lhgO"
                     /locus_tag="VFQ79_04750"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEU0138991.1"
                     /translation="MDIIVIGGGIVGLASALHISSRLPDARITVLEKEVCVGQHQTGH
                     NSGVLHCGLYYKPGSIKARMAVTGIHRMVEFCQENAIPHEICGKLVVATSDAQIPALN
                     DLFERGAANGLQGLRRVCREELREIEPHVAGVAGIHVPQEGIVDYAAVCDAMVRKLNM
                     AGVRVVTGARVDRVRRLGSNWLLETPAGSFEASFLVNCAGLHSDRVSALVSGRRELRI
                     VPFRGEYYKIKPERQYLVRNLIYPVPDPRFPFLGVHFTRLIHGGIEAGPNAVLAFARE
                     GYRKTDISIRDLADTFAYRGFWSFLSRYPGMSWQELRRSFSKERFCRSLQELVPEIGV
                     DDLETGDAGVRAQAIEPDGTLVQDFRFVEDSGALHVLNAPSPAATASLAIGEEIATRV
                     ATAK"
     gene            7241..9244
                     /locus_tag="VFQ79_04755"
     CDS             7241..9244
                     /locus_tag="VFQ79_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0138992.1"
                     /translation="MHPPVPPDEERRLAAVERYHILDTPPEVAFNRITALTAHLFEVP
                     FSSVTFVDRTRQWFKAVHGLEVREAPRDTALCSYAILSNEVLAVLDTTRDPRFSSNPL
                     VTGPPYLRFYAGAPLATPDGFLLGTLNIMDTLPHEDFSGRDRRLLEDLAATVMDEIEL
                     RLVAHHTGDPSSSLIDASPLAIISTGRDGRIRSWNTAAEQIFGWTAAEVIGRPPPIVP
                     DGAADEFDGHRVSVLRGEVLRNIETACCRKDGATIPVNLSAAPLRDQSGEITGSMAII
                     DDITERHLREQQSAHTQKMEAVGQLAGGIAHDFNNLLTVISGYSSLLLQSLAEVDPAA
                     AQLKEIALAADRAAVLTRQLLAFSRKQVLQPLNVDVNTVVRESERMVRHLIGENIELC
                     LALAAEPLRAVVDAGQLVQVVINLVLNSRDAMPDGGVLTIQTSAVEIGPEFVQAHPAV
                     APGAYVMLAVSDTGVGMDQATMSRIFEPFFTTKGPGAGTGLGLATVYGIVTQSNGHIF
                     VYSEPGRGTTFRIYIPRIFDSASTTPSAGSTVAPARTVPTILLVEDESALRKLTRDIL
                     RRSGFKVIEAANGGEALLACEQSSDEISLMIADLVMPGMTGLELAKRLAPLRPAMKVL
                     FMSGYSSHAALASGALEGSLPFLQKPFAPGALTEKIREILNHG"
     gene            complement(9269..9658)
                     /locus_tag="VFQ79_04760"
     CDS             complement(9269..9658)
                     /locus_tag="VFQ79_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0138993.1"
                     /translation="MGVSKKPEGYSSVSPYLVVESAQRVIDFLKKTFDATELRRYGRP
                     DGSIMHAEVRIDDSVIMMGDGVEQWPPFPSSLHVYVDDVDATYARALQAGGTPVQEPQ
                     RKGDDPDRRGGVKDPCGNTWWIATQID"
     gene            10216..11058
                     /locus_tag="VFQ79_04765"
     CDS             10216..11058
                     /locus_tag="VFQ79_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138994.1"
                     /translation="MQRGTVVLLIVIASAAALLISCSSGPAPLRPGTPGFYWVAAKEA
                     YRSGNYVRTSENLARITASENEYKQRAQVWLMVISSGIAGGLMEVADTFEQGARYART
                     NAPVFRRQVAAARASANQAAFQCAETFHEFLEGDKTANLLLEFGYPTGTATEPVQLSK
                     IAKGIVLPAAEYELLEKMMIQRGVVMAASRVSGAGKDYAKAADLFQSGTAQVPREVFV
                     LGMAEALYNQSELYKANKLDLPNRLKLMCDEALEGLDTIPTTSDARKLKTRIQGTIQK
                     IKLS"
     gene            complement(11121..11441)
                     /locus_tag="VFQ79_04770"
     CDS             complement(11121..11441)
                     /locus_tag="VFQ79_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU0138995.1"
                     /translation="MIITTTPGIDGKRIREYRGIVTGEAILGANIFRDLFAGLRDIVG
                     GRSAAYEKELRNAREIAVAEMSASASEQGANAIVGVDIDYEVLGQNNGMMMVSVSGTA
                     VIVE"
     gene            11518..11808
                     /locus_tag="VFQ79_04775"
     CDS             11518..11808
                     /locus_tag="VFQ79_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0138996.1"
                     /translation="MPGILVHGDNHFIVRGPLPDRGTAIALAAHWSIIRIGGTTPAAL
                     QHWRISTKEFREDLEWAVIVPGDGETSAAVAQLLSELAQRGVTIRDASVDSW"
     gene            11866..12564
                     /locus_tag="VFQ79_04780"
     CDS             11866..12564
                     /locus_tag="VFQ79_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0138997.1"
                     /translation="MEPSRILLIDDDVELASLMDEFFTGHGYALKAVYDGPSGLQEAL
                     SGAYDLVLLDVMMPGYDGFEVLRRLREQSRLPVIMLTARTESQSRILGLEAGADDYLP
                     KPFEPLELLARIHAVLRRTRPGEPARAIVLDISGVRLDPNARRVSHLGEDVEMTTVEY
                     DILETLMRSAGRPVSRDELMQRLYQREATPFDRSIDVHISHLRRKFGSRSGLIRTVRG
                     SGYQFVIEETTVGR"
     gene            12574..13860
                     /locus_tag="VFQ79_04785"
     CDS             12574..13860
                     /locus_tag="VFQ79_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0138998.1"
                     /translation="MFRKILLWFAGMLLLSFLGYIATSYWLSQRRVPRGTIFRSTIGY
                     QFTQAIRAYETGGPSALEEELLRMRQFFTGRHFLLDESGRNLVTGEDYSALLKEVPSK
                     RPPPFRRSDRPMLRRTSLDGRYNFVVEFEPRPEPLADLALYGWIVVVIVLLTYVLALT
                     LVRPVRQLHDVVVRFGQGDLSSRVRSRRKDELGELARAFDRMADRIETLLTAERRLLQ
                     DVSHELRSPLARLRFALELSRSNPDSEAALARVDKEVDRLSTLVGELLHVTRAEGDPA
                     ARNLSPIDLAGLLQSLVDDCRIEAHAHGCEIQLNAPGSLQWTGDGELLHRAAENVLRN
                     AISHSPAGTAVRVDLVEDSGQIVVRIRDYGPGVPEDQLSQIFHPFYRVEQDRNRTRGG
                     VGLGLSIAERAVVAHHGEIEARNANPGLLVEIRLPK"
     gene            14021..16978
                     /locus_tag="VFQ79_04790"
     CDS             14021..16978
                     /locus_tag="VFQ79_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0138999.1"
                     /translation="MMRYLCVLIAVFVCGLASAEPQPASTSTLRGTVTDPSAAVVPGA
                     LVVVQSGSESRQATTDGKGQYTLNQIRAGSYTVRITAKGFAPFEVEGYAISDSKVLDV
                     QLTIATEAQTLTVQDEANQVTTEPSSNASAIVLREKDLEALSDDPDELAQQLQALAGP
                     GAGPEGGEILVDGFSGGNLPPKASIREVRINANPFSPEYDRPGFGRIEILTKPGGDRI
                     RGQAFFQFNNEALNSRSPLFAGSTLPPYQQRFFGFNLGGPIRKQKASFGLDVERRSID
                     ENAFILATTLDDRFNPQQVNQAVVAPNIRTSISPRLDYSINAANTLVLRYEHTRMSRE
                     NEGIGDFSLASRGYDQRNTGNVLQLTETAVLSPRAINETRFQYLRTNLSRIGDNSVPA
                     LTVQGAFEGGGAQIGNSGAINNRWELANSTTLTSGKHTVKWGGRLRQAFTDDTSMSNF
                     GGTYTFLGGVGPELDANNQPIDGTSMQLTALERYRRTLLFLAAGYAPAEIRALGGGAS
                     QFSITAGTPAASVNQFDIGLFVNDDWRVRPNLTLSYGLRYETQTNISDFANFSPRLGI
                     AWGIDGGSGRSARTVLRAGFGIFYDRIADSLTLQALRYNGLSQISYFILNPDFYPSIP
                     SIDALTEGQQPVRIQVLDRNLQAPRTYQASIGIDRQLNSYARLSVQYIASRGVHLLRS
                     RNINTPVDGIYPFGDSGPRLLSESTGFSRGNQVVFSPRINYKGLSLFGFYGLSYGKTD
                     VEGQPADPYNLRAEWGPSSFADVRHRAVIGTSLPLPWKITANPFVSMSSGSPYNITIG
                     RDLNGDTLSSERPALLAGLSAAGCSGGDLLYAAGFGCFNLNPAASDSTIGRNSARGPA
                     NFTVNLRLARTWSFGTRGESGMEPGPPRRMGGFRGGSGGGPGRGPGGGGPPPGDGPPP
                     GMFGASSGRKYNLTLSVSAHNLLNSVNYSAPNGDLSSPFFGEYRTLAGFGPFGGGNST
                     YNRKIDLQLRFTF"
     gene            16989..17381
                     /locus_tag="VFQ79_04795"
     CDS             16989..17381
                     /locus_tag="VFQ79_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139000.1"
                     /translation="MITFVILFLLAASGNDTAVEERYVIELTAPPAAEAAARAEPAAE
                     KAAALERARAVALYQQQRVRAELAKWKARVITVTVNLTNTITVEASPAAIAAIETMPE
                     VRSVQPVRVRKTPDSSPRRRWTSVAELR"
     gene            complement(17373..19313)
                     /locus_tag="VFQ79_04800"
     CDS             complement(17373..19313)
                     /locus_tag="VFQ79_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU0139001.1"
                     /translation="MTRNLFLTALLTITLASAESRKIAVSEYREKVYASWLAQCVANI
                     YGLPHENQYIENPGPDQFPLGNSNAAQLRKHNGAYSDDDTDFEYMYLLAMEKFGPEPT
                     YEHLAGLWKHHVRNRVWLANRAALGFMHHGFTPPATGWKQINPHWFQIDPQLVNEIWA
                     VTAPGMVRYAAAKSGWAAEITDSDYGIEPTIHYGAMYAAAFFETDVTKLIDIGNAALP
                     RGSRFRATVEDMKALYRKYPNDWKAARQEMAKRYYHNEPASTKTIWNANLNGAAGILA
                     LLYGRGDFQRTLDLSCSIGFDADNQAATMSGLLGVVHGLKGIPRDLLFPFPDLDWKEP
                     LNDSYKNVTRHDMPDGSLKDMAVRMAEQGERIILKHGGRKIVENGQDYYVINSSATFT
                     PPLEIPAQPAPLIEVGLPVRHPFAMVGPKGAASGITGNLPEGLTAKGLTLSGKASKPG
                     VYPVTLTARSGEAAAQRDFTLAVRPENLASKAERVLAGVPKTDDRFAKMRITVSRSIY
                     ASDVEVIRDGRSLGDGTAFVSVGPAKEPKTDFYGYEWKSEQEIGLIGFHTGVMEETGG
                     WFKDLRVEYRDRDGNWQTVTGVSMTPALPGGNSPYDKPHFAEYLIAFPAVRTTAIRIL
                     GEAGGSEHFTSISELRVYGPLP"
     gene            complement(19310..20305)
                     /locus_tag="VFQ79_04805"
     CDS             complement(19310..20305)
                     /locus_tag="VFQ79_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HEU0139002.1"
                     /translation="MFVIDSTGMAILFCVITMLGWGSWANTQKMSGKDNWPFQLYYWD
                     YAIGVFVLALILLLTLGSAGDRGMSAMDNLAQASGTAIGTAVLSGVLFNIANILLVVA
                     IDAAGLSVAFPVGIGLALVIGTVASYIQEPKGDPGLLFSGVGLVVLAMILSALAYRLL
                     PRSEGKGWARGVVFAVIAGCLMGFFYPQLASSISPDFRSAPIQPGMLTPYTALLFFSL
                     GLLASNFVVNTIFMRAAGRTYSEYFRGSLRLHSLGVLGGAIWMLALGFNLIASGVAGP
                     AISYALGQGATLIAAIWGVLIWREFRNAPQAATTFIVLMFAGYTAGLTLIGMATL"
     gene            complement(20410..21462)
                     /locus_tag="VFQ79_04810"
     CDS             complement(20410..21462)
                     /locus_tag="VFQ79_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0139003.1"
                     /translation="MATIKQVAKAAGVSVGTVSNVLSGSVPVSAELKTRVWAVIRKLD
                     YHPNHVARSLKTKQTRMLGMVIPDFTNPFFPQVVRGAEEVVLKHGYLLVTFNTDDDVK
                     REREVLAILRSRHIDGVLLVVAPNDGDGSHITTAVSAGVRIVCLDRAPAGVTDLDSVF
                     VDNHEGAMLAVRHLIRQGHRRIGMIAGSMLLQTAVERLAGYKAALREAGLKVDQDLVR
                     TGNFRINSGYKCAKDLLLAHRRPSAIFASNGQMALGALKAMEEIRLHCPDDVALVSFD
                     DLPESDLFRPHLTAVAQPAWEIGRQGAELLIQRVEGQLTSETPVVVRLKPELKIREST
                     SKRVSEGVSRRLTRAL"
     gene            complement(21487..22965)
                     /locus_tag="VFQ79_04815"
     CDS             complement(21487..22965)
                     /locus_tag="VFQ79_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU0139004.1"
                     /translation="MTALKLAVSLIAGAVLIAADVDASGPSGRWWERAAAIRRISLKP
                     NRAGETLSLDPVKLGEQLDRMKQQGFDVLEIFAPPEGGFSYAGLDAKDRYRIDPEVGT
                     MEDFRRLVRTAHAKGMAILSFDNLGYSSVEAPHFLKACDDIREGRDTPETRWFLWSDR
                     ADAPPPGTGDTYFMIRPKLPGYDAVKHELWVYSERAKRYYWSKWGGVDSAGNKIRLPQ
                     YNWASREWQEEAGKVVRFWMDTGIDGMVIDAVNWYPGYTWEIGKKRITDVIASYGEKF
                     SQPEGAGGFHEDPVAWVTEGGWNCIQDYGLGIWWEKETEVLRKAIEAGDPRPIEPALR
                     DYHDRVVREGAVLYFEPPTFDDRARHYLAVATLAGIGDLMFFDHRSAPKELDAPLTGI
                     LRLKTEHPALHQLGTRRRMPVRSSDKHYAFLKTAADGSERVLVVLNFQVVPQTVEIDL
                     SGLGNSVLTNLETGESRNRTAWFQVALPAYGYAFFKVEVPAR"
     gene            complement(22982..>24951)
                     /locus_tag="VFQ79_04820"
     CDS             complement(22982..>24951)
                     /locus_tag="VFQ79_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139005.1"
                     /translation="RVFSANLTNEFIFGVGKGQLFNVDQDTRDYMHQTDTLRNQFFQN
                     IGSGEDLGYHEIGFWSNGWPGVGYGEVFMASNPTLQFSDNLSWVKGSHSLKFGFNYFR
                     KEELDWDYIRGVYFSDQFTRAGSVNGSRGGDAMASFMLGLPSGIQQRYQFQGGEPELN
                     FVLPYWGFYAEDRWQVNPRLTLSLGLRYDLPVPVYSGNRYGNAVMDFSYPDWQLMIPG
                     RAEGLSKRYVPVDKNNFAPRISVAFRPTSSLVVRASYGIFYMAGISTGGSVLDNAFGS
                     VPGYVGDWYDNARAGLHDDLPHLQFSDIFPAPASFTLGTYPVSTGTATGYFDYPADVR
                     LSDKMSNTTPYYQRYLLEVQKGLGTNTALSFSYLGGRGTKLPYYEQVNKPEYRTGWTS
                     QDEFNQARPNNSGRFGDVGLLRHGLNSFYNGFTVKVQQNFTRGLQFVSHYTFSKTVAD
                     YNEFTPESLGLNRSAWQWNRKLGRGEAEFSHPHRFVTALSYQVPWGASLNPLAKALVW
                     GWNISAITTFESGNALNVWNGVTEARDYEPNMPNLNGNPNLSRSERRFHRYFNTSVFS
                     APEQDTKGNAGTGIVRGPGINNWDLSFSKTFRPKERLGVEFRGDLLNAFNHAQWGGVD
                     TDFTDSPSSTFGWVTWAREGRIVQLGLRVTF"
BASE COUNT         4964 a         7455 c         7303 g         5229 t
ORIGIN      
        1 aaattcaacg actactacga ctttttcttg aatacgtttg ccacgcccgg ccggcggcct
       61 gaagccgccg ggattcgcgc cggcgccgtc aacacacttg gggaggttcc ggacagcgcc
      121 tggtacacca accggcatgg gcgtaagcgg atgagcatcg aggaattggt gaaagggccg
      181 ggtatcagtc atgagccctc cgaaaaaaag ccctggaaag taaccggtgc gaaactggaa
      241 ggcgtttcgc ccgggtttgc catggaggat gcggagggac ggaaatacct tgtcaaactc
      301 gacccggagt caaatccgga aatggccacc ggcgcggatg ttattggctc caaatttttc
      361 tacgcccttg gttactacac gccggagaac tatatcgtaa cctttcgcaa agaggatctc
      421 accatcaaga agggaaccct gatcgaagac acgcgcggat acgagcggcc gatgaggagc
      481 cgggacattg acgaactgct gctgcacgct gcgcagtacc ctgacggcac ctaccgcgcg
      541 gtcgcaagcc gcttcattgc gggcgagatc atcggcccgt tccgtttcaa cggcgttcgt
      601 aaggatgacc ccaacgatat cgtcgcccac gaaaaccggc gcgatctgcg cggcttgtat
      661 gtcttttcgg cgtggcttgg ccacaacgat gtgaagtcgc tcaatacgct ggactctatc
      721 gtagaggaag gtggtcttcg ttacatccgc cactatctca tcgatttcgg agcggccttc
      781 ggcagcgata gtttcgaagc caagagcccg cgggctggcc accgttatct attcgcatgg
      841 gattccgccg cggcccaggt cttctccctc gggttgtatg tgccggcatg gatgcgtgcg
      901 aactacccta aaatcccggg ggtggggaac tttgaatatg aagtagtcga tgctctcgaa
      961 tggaagccga attacccgaa tccggccttc ctcaacaggc ttcccgatga cgaattctgg
     1021 gctgccagac aggtgatgca gtttacggac gaacagatca acgcgattgt caagagcggg
     1081 cagtatagcg atccgcgcgc tgtcgattgg ctcaccaggt gcctcattga gcgtcgcgac
     1141 aagatcggga gagcctattt cgccaaggtg ctgccgctgg atgaatttcg tgtcgaaaac
     1201 gggatgctga agttccggga tttaggtgtc gactatggtt ttacgcaatc tcgccgctat
     1261 cgaatccgtt ggtcgcgttt cgacaattcg gccgctaggg agagtcccat cccgagcgca
     1321 acgaccttcg agttgccgcg gcagttcgga caagccaggt ctcccgattt tgctgtcgcg
     1381 cgtatccagg ctgatgaccg gcggcaaacc gttctcgtat acttgagatt cacggacggt
     1441 agggcggaga ttgttggcat cgaccggcaa tggaacagcg agaatggcgg cagtggccgc
     1501 cctgactttg ctcgcggcaa ctactctaca aagtgaaaag ttctatccgg acgatccgct
     1561 ctggacggtt ccgaagcccc ggaatgtcga gaagcttagc aagcgtaagc tcaatgacta
     1621 ctacgacttc ttcctgaaca cgtttggacg tcccggtagc aggcacctgc ccggcgaacc
     1681 ggttcgcgcc ttgagtgcga atacactcgg ggaaattccc gatagcgagt ggtacaccaa
     1741 ccgccatgcg aagaaacgta tgtcgctcga ggaactcgcg cggggtccca acaccggaac
     1801 ggggccttcg gaatcggggg attggacgat tatctcggcc aaaaaccagg gcatcacgcc
     1861 gggcttcctg atcaaagacg ctgcgggcgc acgatttttt attaagtttg atcccgtcga
     1921 aaactccgag atggccaccg cggccgatgt catcggctcg aagttttttt acgctttggg
     1981 ctattacgtt ccgcagaact atatcgtcca cttcccacgc tcgcgcttgc gcctggagcc
     2041 gcataccact taccgtgatt cgttcggcaa gcgccgcgaa atgcacgaac gtgatgtcga
     2101 cgagatcctg aggcgcgttc cccgggacga ggacggccgc taccgcgctg tggcaagcct
     2161 gtttttggag ggcgaggtgg tgggtgagtt ccgttaccac ggcacccgcc gcgacgaccc
     2221 caacgatatc atcccgcacg aacaccgccg ggaattgcgc gggctctttg ttttcgccgc
     2281 gtggctgggc catgacgatt cgcgggccat caatacgctg gatacgctgg tcgaagcgga
     2341 tggaacgcgg ttcgtgcggc accatctgat cgatttcgga tccatcctcg gtagcgccag
     2401 cacgtcgtcg aatagtgccc gtagcgggca ccagcacttg ttcgaattca agccctccgt
     2461 ggttgaagtg ctctcgcttg gcctttgggt tccggattgg gcgcgagtcc gctatccgga
     2521 cctgccttcc atcggccgct ttgactgctc catcttcgat cccgagcgat tcaaaaccga
     2581 atacccgaac cctgccttcg aaaaccgctt gccggacgat acgtattggg cggcgaaggt
     2641 ggtcatgtcg tttactcccg acgatattaa agccattgtg aagacaggcg cgtattccga
     2701 ccctgaggcg gaaaggtgga tcgtcgattg tctgcttgtc aggcgtgaac ggattggcag
     2761 gacctacttc gcgaaagtag ttccactgga aaatcttgcg attcgcgaag ggcgtctgtt
     2821 ttttgacgac ctcgccgttc aatatggctt ctcgaagcca cgcgaatacg acatccggtg
     2881 gttttcgctc gacaatcgct cacagcagaa tacggacgtt cccggcagcg gcccggcatg
     2941 gccggcggaa cttcgcgatc ccgcggccac accgtatttc ggagccgtaa tccgagtgcc
     3001 gaaccaacac gccgctctca ccgcttattt ccgccacacg ccacagggtc cgcagttggt
     3061 gggtgtcgag cgcacctggt agaaaccgga tatcaatcca gctcaggagc cgtctgctcg
     3121 ggagtagtca cgcccggcgg cagttccagc agtttgatgt tgcggaaatg gatctccgcg
     3181 ccttccgatt ccaggcacag atagcctttc ttctgggtgg agcgggtgat gccgttcacg
     3241 aatttcccgt tcacggcaag cttgatcacg ccatccaccg ctaccacatc ataggtgttc
     3301 cattccttgt tcccttggac acggttttcg atggacttgc tccgcggccc tcgcggatta
     3361 tccggcaccg tctccacgcc gccaacgccg aacagttcgc cgtgaacata ggccaggggc
     3421 ggggtctcac ctttgatttt gtgaagattc acccattccg ggtccagcat ctgaacctcg
     3481 acaccgtttg gcagacggtt cttttcacct ggaaccgcgt tactccacac aaacacgccg
     3541 gagtttccgc cgggttccat gtgcctccac tcgacatgaa ggatgaagtt ctcgtactgc
     3601 ttctcgcttc gcatcacgcc gatcggacgc cccttgcaaa ccagaatgcc atctttaacc
     3661 gaccacgttt ccgggtccgt attcacgttc acccatccgg tcagatcctt gccgttgaat
     3721 aaatcctgaa aagcgggaac ggcctggcct gtcatccggt ttgcgccggc gatccataaa
     3781 gccgcgccaa acaaagttat tagcagtgca gatctcttca tcaatcgtta acctcgttcc
     3841 gaattatctc gcaaaacgca gatgctttgg ctggaatata ctgatgtggc acatgcacag
     3901 atacctcgga ctcttcttcc tccttgctac cgcctttggg caaacgagcg gtgtcacatg
     3961 gatggccgtg ccggacgccc gctttgaaat cgatggactg ccgtggctct ctgaaaacag
     4021 aggtgagctt ttccggctcc ccgtacggct gaaagacaat ctccccaagg ccgtctgggg
     4081 tctgggcgaa tcgcccagcg ggggccgcat ccgctttcgc acggactcga acaagcttct
     4141 catccggttg gaataccctc gcgcgcccgg catgcgcaac atgcacgcgt tcggacaaac
     4201 cggcgtcgac ctctacctcg acggcgccta tcgcggcacc gccatcgcta ctccggaagc
     4261 gaagacgggc ataccgctcg aacacacata tttcgatttc gaggaccgtc cgcgcgtgga
     4321 acgtgaggtc acgctctatc tcccgctcta catgcccgtg aaagtgcttg gaatcggtgt
     4381 ggacggaaaa gccggcttct ccgctccccg cccgtttgcg cttcccaagc ctgttgtgtt
     4441 ctacggaacc tctatcacgc aaggcggctg tgcgagccgc tccgggttga gctaccaggc
     4501 catcctcggc agaatgttga atctcgattt cgtgaatctc ggcttttccg gaaacggaaa
     4561 gggagaaccc gaggtcgccc ggatggtggc cgagattgaa gcttcctgct tcgttctgga
     4621 cttcgcgcag aataacggca ccgtcgaatc gctggcggcg gtgtatgaac cttttatcgg
     4681 caccttgcgc gagaagtacc ccgaagtgcc catcctggct gttactccca tttcgaatgc
     4741 gcgcgaggcc ctggcacagg atagccggaa cgaaggcatg cggcggcaca tccggcaggt
     4801 ggttagccgg cgcatcgcac aaggcgatac gaacctgcaa ctgatggagg gtaccgacct
     4861 gcttgggccg gaccgcctgg atggactggt cgatggcaca caccccaacg acctcggatt
     4921 tcaatggatg gcggaaggcc tcgccgccag gctcggtaaa ctgctcggcc tggcatctgc
     4981 ttcgaatcgc tgaagccgaa atcgaagtat gatcgaatct gctgtttcag accagagggg
     5041 atacaaatga agagacgaat gcttggcctg ctggcgggtc tgctggtgct gtcctttacc
     5101 ttggccgcgg ctccgtttac gaccatctcc gtattcggag acagcctgtc ggataacggc
     5161 aataccttcg gagttgcgga ctatccgccg ccgccgtact acgagggacg gttttccaat
     5221 gggcccgtgg cggttgagat cttcgccggc atcaccggag ccaatctcga gaaccacgcc
     5281 tggggcgggg caaccacagg gtccgggaac aacgtcgatg gcggcaacgt gtccactgct
     5341 gtagctcttc cgggaatgcg gacggttttc gacgctaccg atttacaacc tgtttcgccg
     5401 gagacgctct ttatcgtgtg gggcgggccg aacgacttcg tggctcctgc gccggaagac
     5461 gccggcaatc ccggtgcgat tgcagcccgc gccgtgggca atttggtgta catcgcacaa
     5521 gggttgcagg cggccggtgc gagcaacatc tgggtgctag ggctgccgga tttgggtctg
     5581 acgccgttcg ttcgctcgca aggggccgcc ctctcgatgg cggcttcgtt tctcacggac
     5641 caattcaatg ccgccctcgc tgctcaaatc tcgctcctcc ccaatgcgcg ttttatcgac
     5701 atcgcgggaa cgcagcgggc gatcatcggg aatccggcgg cctatggttt ctcgaatgtc
     5761 acggatgcct gcctgacgag catgggggtc tgctccgatc ccgggtctta tttttactgg
     5821 gacgacttcc accctacaac cgccgcgcat tccctgctcg cttccgatct ggcagcggtg
     5881 gtggttcccg aacccggcac cctggttctt ctggctggcg ggctcgcggc gctggcagcg
     5941 ttgagacgca cgcgccgctg acgaagtgat ttggcgatga tgggtaggaa tggacatcat
     6001 cgtcatcggc ggcggcatcg tcggtcttgc ctccgcactt cacatcagtt cacggctgcc
     6061 cgatgcacgg atcacggtgc tggagaaaga ggtctgtgta gggcagcatc agaccggcca
     6121 caatagcggt gttctgcatt gcgggctgta ttacaagccc ggctccatca aggctcgtat
     6181 ggcggtgacc ggcatccacc ggatggtcga gttctgccag gagaatgcta tcccgcatga
     6241 gatctgcggc aaactcgttg tcgcgaccag cgatgcacag attcccgcgc tgaacgatct
     6301 tttcgaacgg ggtgccgcca acggtttgca gggcttacgc agggtctgcc gcgaagaact
     6361 acgcgagata gagccccacg tggccggtgt ggcgggtatt cacgtgccgc aggaaggtat
     6421 tgtcgactat gccgcggtct gtgacgcgat ggtgcgcaag ctgaacatgg cgggggttcg
     6481 cgtagttacg ggcgcgcgcg tcgaccgcgt ccggcggctt gggtccaact ggctcctcga
     6541 aacaccagcg ggcagcttcg aagcaagctt tcttgtcaac tgcgccgggt tgcactccga
     6601 ccgcgtcagc gcattggttt ccggccgccg tgagctccgc atcgtgccat ttcgcggtga
     6661 gtattacaag atcaaaccgg agcgccaata cctggtgcgc aaccttattt atccggtccc
     6721 ggacccgcgc tttccgtttc tcggcgttca cttcacgcgg ctcattcacg gcggcattga
     6781 agccggcccc aacgcggtgc ttgcctttgc gcgcgaagga tatcgaaaga cggacatttc
     6841 catccgcgac ctggccgata cgtttgccta tcgtggattc tggtcctttc tttcgcgata
     6901 tcccggaatg tcctggcagg agctgcgccg ctccttcagc aaagagcggt tctgccgctc
     6961 cctccaggaa ctggtgccgg agatcggtgt ggacgatctc gaaaccgggg acgccggtgt
     7021 acgcgcgcag gcgatcgaac ctgacggaac cctggttcag gacttccggt ttgtggaaga
     7081 ttcgggcgcc ctgcatgttc tcaacgcacc cagccccgcc gccaccgcct cgctcgctat
     7141 cggagaagaa atagccacgc gcgtcgccac cgcgaaatag gttccatttt ggtacagtca
     7201 gtggccctat aatgatgcta agttcgctgg tggagagtct atgcatccgc ccgtccctcc
     7261 ggatgaagaa agaagattag ccgccgtaga gcggtatcac attctggaca cgccgccgga
     7321 ggtagcgttc aatcgcataa cggcgcttac ggctcacctg tttgaggtgc ccttttcctc
     7381 ggtgacgttc gtcgacagga cccgccagtg gttcaaggcc gttcacggac tggaggtgcg
     7441 agaggctcct cgcgataccg cgctttgctc ttacgctatt ctttccaacg aagtgctggc
     7501 ggtattggac accacccggg atccgcgctt cagctccaat ccgctggtga ccggcccacc
     7561 ctacctgcgt ttctatgccg gcgctccgct cgctacgcca gatggttttc tcctcggaac
     7621 gctaaacatc atggatacgc ttccgcacga agatttttcc ggccgggacc ggagactcct
     7681 ggaagatctg gcggcaacgg tgatggacga gatcgagctc cgtttggtgg cccaccatac
     7741 cggcgaccca tcgagcagtt taattgatgc ttcccctctt gcaatcatct ccaccggccg
     7801 ggatggcagg atacgcagtt ggaataccgc cgcagaacag atcttcggct ggaccgcggc
     7861 cgaggtgatc gggcggcctc cgccgattgt gccggatggc gccgcggacg agttcgacgg
     7921 gcatcgtgtt tccgtattgc gtggtgaagt ccttcgcaat atcgagaccg cctgttgccg
     7981 gaaagacggc gccacgattc cggtcaacct gtcggcggcg ccgctacgcg atcaatccgg
     8041 cgaaatcacc ggcagcatgg ccattatcga tgacattacg gagcgtcact tgcgggagca
     8101 gcaatcggcg cacacacaga agatggaagc cgtagggcaa ttggccggag gcatcgctca
     8161 cgacttcaat aacctcctga cggtgatctc cggttattcc agcctgctgc tgcaatcgct
     8221 ggcagaagtg gatcccgcgg cggcccagtt gaaggaaatt gcgctggccg ccgaccgcgc
     8281 tgccgtgctc acccgccagt tgctcgcctt cagccgcaag caggtcctcc agccgttgaa
     8341 cgtcgatgtc aacaccgttg ttcgtgaatc tgaacgtatg gtgcggcatc ttatcggcga
     8401 aaacattgag ctttgcctgg cgctcgcggc tgaaccgctg cgagcggtcg tcgatgccgg
     8461 gcaactcgtg caggttgtca taaacctggt gctcaattcc agggatgcga tgccggatgg
     8521 aggtgtcctg acgattcaga catccgcggt tgaaatcgga cccgagttcg tacaggcgca
     8581 tcccgctgta gctccaggcg cttatgtgat gctggcggtc agcgataccg gtgtagggat
     8641 ggaccaggcc acgatgtccc gcatctttga gccgttcttc acgacgaaag gccccggagc
     8701 gggaaccgga ttgggacttg ccacggtgta tggcatcgtt acgcagagca atgggcacat
     8761 attcgtctat agtgagccgg ggcgtggaac caccttccgc atctatatcc cgcgtatttt
     8821 cgattctgcc tccaccacac cttctgccgg ttccacggta gccccggcac gcaccgtccc
     8881 caccatactt ttggtggaag atgagtctgc cctgcggaaa ttgacccggg acattctgcg
     8941 gcgctccggc tttaaagtga ttgaagctgc caatggcggc gaagccctgc tcgcgtgtga
     9001 gcagagttcg gatgagatct ctttgatgat cgcggacctg gtcatgccgg gaatgaccgg
     9061 gctcgaactt gcaaaacgcc tggcgcccct gcggccggca atgaaggtgc tcttcatgtc
     9121 aggctacagc agccacgccg ctctcgcctc cggcgcgctg gaaggcagtc tgcccttcct
     9181 tcaaaagccc ttcgccccgg gggctctgac agaaaagatc cgcgagatcc tgaatcacgg
     9241 ttgagggtat cgcgcggccc cggtgccctc aatctatttg tgtcgcaatc caccaagtgt
     9301 tgccgcaggg atctttcaca ccgccacgcc ggtccggatc gtctcccttg cgctgcggct
     9361 cctgcaccgg tgtgccgccg gcctgcaacg cccgcgcgta cgtggcgtcc acatcgtcga
     9421 catagacatg cagcgacgaa ggaaatggcg gccattgctc taccccgtcg cccatcataa
     9481 tcaccgagtc gtcgatgcga acttcggcat gcatgataga gccatccggc cggccgtacc
     9541 ggcgaagttc ggtggcatcg aatgtcttct tcaggaaatc gataacgcgc tgtgcgctct
     9601 cgacaaccag atatggagaa acgctgctgt atccctctgg tttttttgaa acgcccatgg
     9661 tgaaatccta gcaggttccg aaaaagctgc gtcatccggc ataggttcca ggttcttgag
     9721 ggtcttgtac tagcaggcct tatactccac tttagttttg cgctttcagg gtgtaaccta
     9781 tatgcggagc ccgtttggcc cggaaaggag aatatctccg cgatgggcga tctcgccatt
     9841 tgtactccgt gccagacatc ctgcgcttcc gggaggcggc catgagccgt atccttgcgg
     9901 cggccgccgc tcccgggcgc tcgatattgc atctggcatt cttcaaggtt cgccgcgact
     9961 cccggtgttc gtctgcttgc gtttgggcaa ttacttgtcc cgaatccttt cgatcagctc
    10021 tcgtacaggc agacacaccg cggcagttca caggaccatt gatagatctg cgtatccatg
    10081 actgcacgct gtggccgcgg tataagaact gcgcccgcgc gtgcctggaa gtgattgtca
    10141 acaattccgg cgagcgcttc gctggcatgg gtccggcgaa gcacggcggc tgatgtcaga
    10201 cgtagaggcg atgtcatgca aagaggaact gttgtcttgt tgattgtcat tgcttcggcg
    10261 gcggcgctcc tgatctcttg cagttccggt cccgcaccac tgaggccggg gacccccggg
    10321 ttttattggg tggccgcaaa agaggcttac cgttcgggca attatgtgcg gacaagcgag
    10381 aatctggctc gcattacggc gagcgagaac gagtataagc aacgcgcgca agtgtggctg
    10441 atggtgattt cctccgggat tgccgggggg ttgatggaag tcgccgatac tttcgagcaa
    10501 ggggccaggt acgcgcgcac caatgccccc gtttttcgca ggcaggttgc tgccgcccgg
    10561 gcttcggcta accaggctgc cttccaatgc gccgaaacgt ttcacgaatt tctggaagga
    10621 gacaagactg cgaatcttct gctggagttc ggttacccca ccggcaccgc tactgaaccc
    10681 gtacagttga gcaaaatcgc aaaggggatc gtcttgcctg ctgcggaata tgaactgctc
    10741 gagaagatga tgattcagcg cggggtagta atggctgcca gccgtgtctc gggcgccggg
    10801 aaggattacg ctaaggccgc ggatttattc cagagcggca cggctcaggt gcccagggaa
    10861 gtattcgtac tcggcatggc agaggcgctc tacaatcagt ccgaattgta caaagccaat
    10921 aagctggatc tgccgaaccg cctgaaactg atgtgcgatg aggctctgga aggcttggac
    10981 accattccga cgacctcgga cgccaggaaa ctgaagacca ggatccaggg cacgatacag
    11041 aagatcaaat tgagttagca ccggcgaaac cacggaacgg catttgtccg cgcgggtaag
    11101 gggtgtggtt tgtatagaat ctattccacg atgacggcag ttccgctgac actcaccatc
    11161 atcatgccgt tgttctggcc gagcacctcg tagtcgatgt cgacgcccac aatcgcgttc
    11221 gccccttgct ccgacgcgct ggcggacatc tcggccacgg cgatttcgcg cgcgttccgc
    11281 aactctttct cataagccgc cgaacggccg ccgacgatat cgcgaagccc ggcgaaaaga
    11341 tcacggaaga tattcgcccc cagaatcgcc tctcccgtga cgattccccg atactcccga
    11401 atgcgtttgc cgtcaatacc cggtgttgtg gtgataatca tatggcgtta tacgaatttc
    11461 gctaagagtc cgttccgtca atcctcgttc cgctccggct atgctgttaa gagccgcatg
    11521 cccggcatcc ttgttcacgg cgacaatcac ttcattgtgc ggggcccttt gccggatcgc
    11581 ggcactgcga tcgcgttagc cgcccattgg tccatcattc gcatcggagg gacaactccg
    11641 gctgctctcc aacactggcg cattagtacc aaagagtttc gcgaggacct cgagtgggcc
    11701 gttatagtgc ccggcgacgg cgagacgtcc gctgctgtcg cacagttgtt gagcgaactg
    11761 gcgcagcggg gtgtcaccat tcgcgacgcg agcgtcgact cgtggtaagg acgccgcgct
    11821 tcgtgctgcc tgcgctttta agagagcggg tggacaatgt aagtggtgga accaagccgc
    11881 attctcctga tagacgacga cgtcgaactg gcttcgctga tggatgagtt ctttaccggg
    11941 cacggctacg cattgaaagc ggtgtatgat ggcccatcgg gtttgcagga ggccctgagc
    12001 ggcgcctacg acctcgtgct gctcgatgtg atgatgccgg gttatgacgg tttcgaggtg
    12061 ctgcgccggt tgcgggagca aagccggttg cctgtaatta tgttgaccgc gcgcacggaa
    12121 tcccagagcc gtattctggg actcgaagcg ggtgccgacg attacctgcc aaaaccgttt
    12181 gagccgctgg aattgcttgc gcgcattcat gcggtcctgc ggaggacacg gcccggcgag
    12241 cccgcgagag ccatcgtgct ggacatcagc ggcgtgcgtc tcgaccccaa cgcaaggcgt
    12301 gtttcgcacc tcggggaaga tgtcgagatg acgacagtgg aatacgacat cctcgaaacc
    12361 ctgatgcgtt cggcgggccg gccggtctcc cgcgatgaat tgatgcagcg cctctatcag
    12421 cgcgaggcaa cgcccttcga caggtcgatc gatgttcaca tcagtcacct gcggagaaaa
    12481 ttcgggagcc gatccggcct gattcgcacc gttcgcggct ctggctatca gttcgttatc
    12541 gaagagacca cggtgggtcg ctgaggcgcg cctatgtttc gaaagattct cctctggttc
    12601 gcgggaatgc tgctgttgag cttcctgggc tacatagcca cgtcgtactg gctgagccag
    12661 cgccgtgttc cgcgcgggac gatttttcga agcacgatag gatatcagtt cactcaagct
    12721 atacgcgcct atgagaccgg tggaccttcg gctctcgaag aagagttgct tcgcatgcgg
    12781 caattcttca ccggacggca tttcctgctt gacgagagtg ggcgaaacct ggtgaccggc
    12841 gaagactact cggccttgct caaggaggtt cccagcaagc gtccgccccc gttcaggcgg
    12901 tctgaccgtc caatgctcag aaggacctcg ctcgacggac gctacaactt cgtcgtggag
    12961 tttgaaccac gcccggaacc gctcgccgac ctcgctctgt acggatggat cgtggttgtg
    13021 atcgtcctgt tgacctatgt gctggcgctc acgctggtga ggccggtgcg gcagcttcac
    13081 gatgttgtcg tgcggttcgg acaaggcgat ctttcctcgc gcgtacgctc tcgccgcaag
    13141 gacgaattgg gggaactggc gcgtgcgttc gatcgtatgg cagaccgcat cgagacgttg
    13201 ctgaccgccg agcggcggct gttgcaggac gtttcgcacg aactgcgctc accgcttgcg
    13261 cggctgcgtt tcgcgctgga gctgtcccgg tcgaatcccg attccgaagc ggctctggcg
    13321 cgcgtggaca aagaggtgga ccggctttcc acgctggtcg gcgagctgct ccacgtgacg
    13381 cgtgccgaag gggatcccgc tgccaggaac ctgtcaccga tcgatctcgc cggattgctc
    13441 caatccctgg tggacgattg ccggatagag gcccatgcgc atggttgcga gatacaactg
    13501 aacgcgcccg ggagcttgca gtggacgggt gacggcgaac tgctgcaccg ggcggcggag
    13561 aacgttctgc gaaacgcgat cagccattcc cccgcgggaa cggccgtgcg cgtggatctg
    13621 gtcgaagact ccgggcaaat tgtcgttcgg attcgcgact atggtcccgg tgtgcccgag
    13681 gatcaattga gccagatttt tcacccgttc tatcgcgtcg agcaggaccg gaaccgcacg
    13741 cgcggtggtg tgggactggg actgtctatt gcggagcgcg cggtagtggc acatcatggc
    13801 gagattgagg cgcgaaacgc gaatcccggt ttgctcgtcg agatccggtt accgaagtaa
    13861 ttctcccctc gcagaggctt ttacaaaact taactacccg aattgcgatt ctgccgttta
    13921 atgaaagtgt aaggaccgct accgcaaaac cggccgcctg ctgacgcaaa cggctcagac
    13981 ggaagcgcaa cttcacgtca gaactaacga aaggacgttc atgatgaggt atctgtgtgt
    14041 cctcatagcg gtattcgtgt gcggtttggc gtccgcggag ccgcagcccg cttccacttc
    14101 cacattacgc ggcacggtta cggatccctc ggcggcggtc gtgccgggtg ccttggtcgt
    14161 cgtgcaaagc gggtcggaat cgcggcaggc cacgacagac ggcaaagggc agtacacact
    14221 caaccagatt cgagcgggca gctacacggt ccgcatcacc gcaaagggct tcgcaccgtt
    14281 tgaagtcgag ggctacgcga tttcggattc taaagtactc gacgtccaac tcaccatcgc
    14341 cacagaggcg cagaccctca ctgtgcagga tgaagccaat caggtgacga ccgaaccaag
    14401 ctcgaacgca agcgctattg tgctgcggga gaaagacctc gaagcgctat ccgacgaccc
    14461 ggatgaattg gcgcagcagc tacaggcgtt ggctggtccg ggtgcgggtc cggaaggtgg
    14521 cgagattctc gtagacggct tcagcggagg gaatctgcca cccaaggcgt cgatccgtga
    14581 ggtccgtatc aatgcgaatc ctttctcgcc ggaatacgat cgcccggggt ttggccgcat
    14641 tgagattctg acgaagccgg gcggcgatcg tattcgcggt caggcttttt ttcaattcaa
    14701 caatgaagcg ctgaactcgc gcagccctct gtttgcaggt tcaacgctgc ctccctacca
    14761 gcagcgcttc ttcgggttca acctgggcgg tcccatccgg aagcagaaag cctccttcgg
    14821 tctggacgtg gagcgacgca gtatcgacga gaacgcattc attctggcta caacgctgga
    14881 cgatcgcttc aatccgcaac aggtcaatca ggctgttgtt gcgcccaata tccgcaccag
    14941 catcagcccg cggcttgact attccattaa tgctgccaac accctcgtgt tgcgctacga
    15001 acacacgcgt atgagccgag agaacgaggg aattggagac ttcagcctgg cctcgcgcgg
    15061 gtatgaccag cgcaatacgg gaaacgttct ccagcttacg gaaacggcag ttctgagtcc
    15121 tcgggccatc aacgaaacgc gcttccaata tctccgcacc aacctgtcgc gaatcggcga
    15181 caattcggtt cctgcgctta cggtgcaggg agcgttcgaa ggcggcggcg cacaaattgg
    15241 aaactcggga gccatcaaca accgctggga gctggcgaat tccactactc ttacgtcggg
    15301 caaacacacg gtgaagtggg gaggaagact gcgccaggcc tttaccgacg acacctccat
    15361 gagcaacttc gggggcactt atacgtttct tggcggcgta gggcccgaac tcgatgcgaa
    15421 taaccagccc atcgatggaa cctccatgca gttgaccgca ctcgaacgat accggcgcac
    15481 tctgctgttt ctcgccgccg gttatgcgcc ggcggaaatt cgggcgcttg ggggaggcgc
    15541 ttcgcaattc agcatcactg ccggtacgcc ggccgcatcc gtgaaccagt tcgacatcgg
    15601 actgtttgtc aacgacgatt ggcgcgtgcg gcccaatctc accctcagct acggactgcg
    15661 atatgagacg cagaccaaca tcagcgattt tgcgaacttt tctccgcgcc ttggaatcgc
    15721 ctgggggatt gacggcggct cgggccgttc tgccaggacg gttctcagag ctggttttgg
    15781 catcttctac gatcgcatcg ccgactcgct tacgcttcag gctcttcgtt ataacggtct
    15841 gagccagatc tcgtacttta ttctgaatcc ggatttctat ccttccattc cttccattga
    15901 tgcgcttacg gaaggacaac agccggtgcg catacaagtg ctggatcgaa atcttcaagc
    15961 cccgagaacc taccaggcga gcatcgggat cgaccggcag ctcaactcct acgcgcgcct
    16021 gagtgtgcaa tacatcgcca gccgcggtgt gcatctgctg cggtcgcgaa acatcaacac
    16081 gccggttgac ggcatatatc cgttcggcga ttccggcccg cgcctgctga gcgaatcgac
    16141 gggcttcagc cgcggcaacc aggttgtgtt cagccccagg atcaattaca aaggattgtc
    16201 cctgttcggc ttctacgggc tgtcttatgg gaagacggat gtggaagggc aacctgccga
    16261 tccttacaat ttgcgagctg aatggggacc ctcgtctttc gccgatgtgc ggcaccgcgc
    16321 cgtgatcggc accagccttc cgttgccctg gaaaatcacc gccaacccat ttgtatcgat
    16381 gagcagcggc agcccttaca acatcaccat cgggcgcgat ttgaatggcg acacgctaag
    16441 tagtgaacgg ccggcgctgc ttgctggcct gagcgccgcg gggtgctcgg gcggcgattt
    16501 gctatatgcc gccggtttcg gttgcttcaa tttgaatcct gccgcctcgg attcgaccat
    16561 cggacgcaac tccgcgcgag gaccggcgaa ctttaccgtc aacctccgcc ttgcccgcac
    16621 gtggtcgttt ggcactcgtg gggaatcggg aatggaaccc ggtcctccgc ggcgaatggg
    16681 aggttttcgc ggcggctctg gtggcggtcc cggccgcgga ccgggtggag gaggccctcc
    16741 gccgggcgac ggccctcctc ccggtatgtt cggtgcgtca agcggtagga aatacaatct
    16801 gacgctgagc gtatcggcac ataaccttct caacagcgtg aactacagcg cgccgaatgg
    16861 cgatctttcg tctccgtttt ttggagagta ccggacgctg gcaggcttcg gaccgttcgg
    16921 aggaggaaat tccacctaca accggaagat cgacctgcaa ctccgattca cgttttgaag
    16981 caacgatcat gattactttt gtgattcttt tcctgttggc cgcgtccggc aatgacacag
    17041 cggtggaaga acgatacgtc atcgaattaa ccgcgcctcc agccgctgag gccgcggcgc
    17101 gggccgagcc ggcggcggag aaagcggcag ccttggagag agcgcgcgcc gtcgcgctct
    17161 accagcaaca gcgagttcgg gcggagcttg caaagtggaa ggcccgcgtc atcactgtga
    17221 cggtaaacct cactaacacg atcaccgtcg aagcttcgcc cgccgccatc gccgcgatcg
    17281 aaacgatgcc ggaagttcgc agcgtccagc ctgtccgcgt ccgcaagacg ccggattcct
    17341 cgccccggcg gcgctggacg tctgttgccg aattaaggta gcggtccgta gacgcgcagt
    17401 tccgagatgg aagtgaaatg ctcgcttccg ccggcttctc caagaatgcg aatcgccgtt
    17461 gtgcggaccg cggggaatgc aatcaggtac tcggcgaaat gcggcttgtc atacggactg
    17521 ttgccgccag gcaaagccgg ggtcatggag acacccgtta cggtctgcca gttcccgtca
    17581 cgatcccggt attccacgcg gaggtccttg aaccatcccc cggtctcttc cattacgcct
    17641 gtgtggaaac caatcaggcc gatctcctgc tccgatttcc actcatatcc gtagaagtct
    17701 gtctttggtt ccttcgcagg cccgacgctc acaaacgcgg tgccatcgcc gagagagcgt
    17761 ccatcgcgaa tcacttctac atcggaggcg taaatcgacc ggctgaccgt gattcgcatc
    17821 tttgcgaagc gatcgtccgt cttcggcacg ccggcaagaa cacgctccgc tttggacgcc
    17881 aggttttccg gacgaacggc gagcgtaaaa tcacgttgtg ctgccgcttc gccggaacgg
    17941 gctgtcagag tcacgggata gacaccgggc ttcgaagctt tgccggaaag tgtcaagcct
    18001 ttggccgtca agccttccgg cagatttccg gtgataccgg atgcagcacc ctttggacct
    18061 accatcgcga acggatggcg gaccgggaga ccaacttcga tgagcggagc cggctgcgcg
    18121 gggatttcga gtggcggcgt gaacgttgcg ctggagttga ttacatagta atcctggcca
    18181 ttttcaacaa tcttacgccc gccgtgtttg agaatgattc gctcgccctg ttccgccatg
    18241 cgcacggcca tgtctttcaa gcttccatcc ggcatgtcgt ggcgcgtcac gttcttataa
    18301 ctgtcgttca acggttcctt ccagtcgaga tccggaaacg ggaacagcag gtcacgtgga
    18361 atgcctttga gaccatggac gacaccgaga agccccgaca tcgtggctgc ctggttgtcc
    18421 gcgtcgaaac cgatggagca ggagagatcg agcgtgcgct gaaaatcgcc gcggccgtac
    18481 agcagcgcga gaatgcccgc ggcgccgttg agattggcgt tccagattgt tttcgtgctt
    18541 gccggttcgt tgtggtagta acgtttcgcc atctcctgac gcgctgcctt ccagtcattc
    18601 gggtacttgc gatagagcgc cttcatgtcc tcgacggtcg cccggaaccg gctgcctctg
    18661 ggcaacgcag cgttgccaat gtcgatcagc tttgtcacgt cggtttcaaa gaaggcggcg
    18721 gcatacatgg cgccgtagtg aatcgtcggc tctatgccat agtccgagtc tgtaatctcc
    18781 gcggcccaac cgctcttcgc cgcggcgtaa cgaaccatgc ccggggccgt caccgcccag
    18841 atttcattca ccaattgcgg atcgatctgg aaccagtgcg ggttgatctg cttccatccg
    18901 gtcgccggcg gggtgaatcc atggtgcatg aagcccagtg ccgcccggtt cgccagccag
    18961 acgcgattgc gcacgtggtg tttccataag ccggcgagat gctcatacgt tggttccggc
    19021 ccgaacttct ccatggcgag caaatacatg tactcgaagt ctgtatcgtc gtcggagtag
    19081 gcaccattgt gtttccgcag ttgggcggca ttcgagttac cgagcgggaa ctggtcgggg
    19141 cccgggttct cgatatattg attttcgtgc ggcagcccgt agatattcgc cacgcattgt
    19201 gccagccagg aggcgtagac tttctcccgg tactcggaaa ccgctatctt cctggactcg
    19261 gcggaggcca gggttatcgt cagaagcgca gttaagaaca aatttcgagt catagtgtcg
    19321 ccattccaat aagagtgagc ccggccgtgt agcccgcaaa catcagcaca atgaaggtgg
    19381 tggccgcttg cggggcgttg cggaactcgc gccagatcaa tacaccccag atcgcggcaa
    19441 tcaaggtcgc gccttgtcct agtgcgtacg agatggccgg cccggcaacg ccggaggcga
    19501 tcaggttaaa gccgagagcg agcatccaga tagcgccgcc gagcacgccg agcgagtgca
    19561 gccgcagcga tccgcggaag tactccgagt aggtgcgccc cgcggcccgc atgaaaatgg
    19621 tgttcacgac aaagttgctc gccagcagtc cgagcgagaa aaacagcaga gccgtatagg
    19681 gtgtcagcat gccgggctgt atgggagccg agcggaagtc cggcgatatc gagctcgcaa
    19741 gctggggata gaaaaagccc atcaggcagc ccgcgataac ggcaaagaca acgccgcgcg
    19801 cccaaccttt accctcgctg cgaggcagca gccggtaagc cagcgcggac aggatcatcg
    19861 ccagtacgac caggccgacg ccgctgaata ataatcccgg gtctcctttc ggttcctgga
    19921 tgtagcttgc aacggtcccg ataacgagcg ccagcccgat acccacagga aatgcaacgg
    19981 acaggcccgc cgcatcgatg gcgaccacca gaagaatatt cgcgatgttg aagagcacac
    20041 cgctcagtac agccgtgccg attgccgttc ccgaggcttg tgccaggttg tccattgcgc
    20101 tcatgccgcg gtcgcccgcg ctgcctagcg tcaacagcag gataagagcg agcacgaaga
    20161 cgccgattgc atagtcccag tagtagagtt gaaaaggcca attgtctttg cccgacatct
    20221 tctgcgtgtt ggcccacgag ccccacccaa gcatcgtgat tacgcaaaag agaatcgcca
    20281 ttccagtgct gtcaatcaca aacatcgtgc cacctgcctg cctgtaagaa tgtcattttt
    20341 cagatccttg aatcgattca attttattgc tttgcttcag taagctctcc ggtgccaccg
    20401 cggcgcccgt taaagcgcgc gtgtcaggcg gcgggatact ccttctgaca cacgcttcga
    20461 tgtggattcg cgaattttta actccggttt cagccgaacc actaccgggg tttcggaagt
    20521 cagttgacct tccacgcgct ggatcagcaa ttccgcacct tgccgtccga tttcccaggc
    20581 aggctgggcg accgcggtca aatgggggcg gaacagatcg gattcaggaa ggtcgtcgaa
    20641 ggaaaccagc gcgacgtcgt cggggcaatg cagtctgatc tcttccatcg ccttcagcgc
    20701 ccccaaagcc atctggccgt ttgacgcaaa aatcgcactg gggcgccgat gagccagcaa
    20761 caaatccttg gcgcatttgt agccggagtt gatccgaaag tttcccgttc gaaccaaatc
    20821 ctggtccact ttgagcccgg cctcgcgcaa cgcggcctta taaccagcga gccgctcgac
    20881 cgccgtttgc aagagcatgg agccggcgat catgccgatg cgccggtgcc cctgtcgtat
    20941 aagatgccgc actgcaagca tggcgccctc atgattgtcg acgaacaccg aatcgagatc
    21001 cgtaacgcct gccggagcac gatccaggca cacgatccgc acgccggccg agacagcagt
    21061 cgtgatgtgc gatccatcgc cgtcgtttgg ggcgaccacc aacagcaccc catcgatgtg
    21121 ccgtgaccgc aggatcgcca gaacttcacg ctctcgcttt acatcgtcgt cggtgttgaa
    21181 ggtgaccagc agatatccat gcttcagcac gacttcttcc gcaccgcgga ccacttgggg
    21241 aaagaaggga ttcgtgaagt cgggtatcac catcccgagc atcctggtct gcttcgtttt
    21301 caggctgcgg gcgacatggt tcggatgata atcgagtttg cggataacgg cccaaacgcg
    21361 cgtcttcaac tcggcactca ccggtacaga cccggaaagt acattggaga ccgtgccaac
    21421 ggacacgccc gccgcttttg ccacttgctt gatcgttgcc atatccggat ggttccgcct
    21481 tctatactat cgggccggga cctccacttt gaagaacgcg tacccgtacg ccggaagggc
    21541 cacctggaac caggctgtcc ggtttcgcga ctcgcccgtt tcgaggttgg tcagcacaga
    21601 attgccgagc ccacttagat cgatttcgac cgtctgcggc acaacctgga aattcagaac
    21661 gaccagcacc cgttcggaac catccgcggc cgtcttcaga aaagcatagt gcttatcgct
    21721 cgagcgaacc ggcatccggc gcctcgtccc gagttgatgc aatgccggat gctccgtttt
    21781 cagacggaga atcccggtaa gcggcgcatc cagttccttc ggagcgctcc tgtggtcgaa
    21841 gaacatcagg tcgccgatgc ccgccagtgt tgcgaccgca aggtaatgcc gggcgcgatc
    21901 gtcgaaagtg ggcggttcga aatacaggac agcgccttcg cgcaccactc gatcgtgata
    21961 gtctcgcaac gccggctcga tgggccgcgg atcgcccgct tcaatggcct tgcgcagaac
    22021 ctcggtttcc ttttcccacc agatcccgag cccgtaatcc tggatgcaat tccagccgcc
    22081 ttcggtcacc catgccaccg gatcttcatg gaatccaccg gcgccttccg gctggctgaa
    22141 tttttcaccg tagcttgcaa tgacgtcagt gatccgtttt tttccaatct cccaggtgta
    22201 gccgggatac cagttgacgg cgtcgatcac catgccgtcg atgccggtgt ccatccagaa
    22261 tcgtacgacc ttcccggctt cctcctgcca ctcccggctg gcccagttgt attgtggaag
    22321 acgtatctta ttgccggccg aatccacgcc gccccacttc gaccagtaat agcgctttgc
    22381 acgctcgctg tagacccaga gctcgtgctt gaccgcgtcg taaccaggga gcttcgggcg
    22441 gatcatgaaa taggtatctc cggtgccggg cggcggtgca tccgcgcgat cgctccacaa
    22501 aaaccaacgt gtctccggcg tgtcgcgccc ctcccgaatg tcatcacacg ccttcagaaa
    22561 atgcggcgct tccacgctgc tgtagccgag attgtcgaac gagagaatcg ccatcccctt
    22621 ggcgtgggcg gtccgtacca ggcggcggaa atcctccatg gttcccactt ccggatcgat
    22681 ccggtaacgg tccttggcgt ccagaccggc gtagctgaaa ccgccttccg ggggcgcgaa
    22741 gatttccagc acgtcgaagc cctgctgctt catgcgatcc aattgctcgc cgagtttcac
    22801 cgggtcgagc gacagcgttt cacctgcacg attgggcttc agagaaatcc ggcgaatggc
    22861 cgccgcccgt tcccaccacc ggccagaggg accggaagca tccacatcag cggcgatgag
    22921 aacggcgccg gcaatcaggg agaccgcaag ttttaaagct gtcaaggcgc ggcctctggt
    22981 gtcagaaggt cacccgcagc cctaactgga cgatgcggcc ttcgcgagcc caggttaccc
    23041 atccgaaagt gctgctcggc gaatccgtaa aatcggtgtc gacgccgccc cactgggcat
    23101 gattgaaagc gttcaggaga tctccccgga actccacgcc cagtctctct tttggccgaa
    23161 aggttttcga gaacgacagg tcccagttgt tgattccggg cccacgcaca attcccgtac
    23221 cggcattgcc tttcgtatcc tgctccggcg ccgagaaaac gctcgtattg aaatagcgat
    23281 ggaagcggcg ttcgcttcgc gagaggttgg ggttcccgtt caagttcggc atgttcggct
    23341 cataatcccg ggcctctgtc acgccgttcc agacattgag tgcattcccc gattcaaagg
    23401 tcgtaatggc gctgatgttc catccccaaa ccagggcctt tgcaagggga ttcaggctgg
    23461 cgccccatgg cacctggtag ctcagggccg tgacaaagcg atgcggatgc gagaattcgg
    23521 cctcgccgcg gccgagcttc cggttccatt gccacgcgga ccggttgagt cccagcgatt
    23581 cgggagtgaa ttcattgtaa tcggcgacgg ttttcgaaaa ggtgtaatga ctgacgaatt
    23641 gcaggcccct ggtgaagttc tgctggacct ttactgtgaa accgttgtag aaggagttca
    23701 gtccgtgccg gagcagtccg acgtcaccga agcgcccgct gttgttcgga cgcgcctggt
    23761 tgaactcgtc ctgcgatgtc cagccggtcc gatactcggg tttattcacc tgctcgtaat
    23821 aggggagctt cgtcccgcga ccgccgaggt aagaaaacga gagggcggta ttggtgccca
    23881 agcccttctg gacttcgagc aggtatcgct ggtaataagg cgtcgtgttg gacatcttgt
    23941 cggagagacg cacatcggcg ggatagtcga agtagcctgt ggcggtacct gtgctgaccg
    24001 gataggtgcc cagggtaaag ctggcgggcg ccggaaagat gtcgctgaac tgcaagtgcg
    24061 gcaggtcgtc gtgcaagccc gcgcgcgcat tgtcgtacca gtcgccgacg tagccgggca
    24121 ccgacccgaa cgcgttgtcc agcacactgc cgccggtgga gatgcccgcc atataaaaga
    24181 tgccgtagct ggcccttaca accagtgacg acgtcggccg gaaagccaca ctgatacgcg
    24241 gagcgaagtt gtttttgtcg acggggacgt accgtttcga cagcccttcg gcgcggcccg
    24301 gaatcatcaa ctgccagtcc gggtaagaga agtccatcac ggcgttgccg taccggttgc
    24361 ccgaatagac gggcacgggc aggtcatagc gcaagcccag gctgagggtc aaccggggat
    24421 tcacctgcca tcggtcttcg gcgtagaacc cccaataagg aaggacgaaa ttcagctccg
    24481 gctcacctcc ctgaaactga tagcgctgct gaatgccgct cggcaatccc aacatgaagg
    24541 acgccatcgc atctccgccc cggctcccat ttacgcttcc ggcacgcgtg aactgatcgg
    24601 agaagtagac tccgcggatg tagtcccaat ccagttcttc tttccggaaa tagttgaagc
    24661 cgaacttcag cgaatggctt ccttttaccc aactcagatt gtcggagaat tgcagtgtgg
    24721 ggttggatgc catgaagacc tctccatatc ccaccccggg ccagccattg ctccaaaaac
    24781 cgatctcgtg atatccgaga tcttcaccgg agccgatgtt ctgaaagaac tggttccgca
    24841 gtgtatcggt ctggtgcatg tagtcccgcg tatcctgatc gacattgaaa agctggccct
    24901 ttccgacgcc gaagataaac tcgttggtca gattggccga gaacacgcgc g
//
LOCUS       DASTMK010000117         5562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_38291, whole genome shotgun sequence.
ACCESSION   DASTMK010000117 DASTMK010000000
VERSION     DASTMK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38291"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFQ79_04825"
     CDS             <1..745
                     /locus_tag="VFQ79_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU0139006.1"
                     /translation="ITMRRFGRKLDVERMRAELPLQPFWFDLLHINGADVTEEPQTRR
                     FAALREIASEENIIPRIMTADSAAARDFLLLALKHGHEGIMAKALSAPYAAGKRGQGW
                     LKVKQARTLDLVVLAAEWGHGRRRGWLSNLHLGARDTKRGGYAMLGKTFKGLTDAMLS
                     WQTQELLKFEIARDEYTVYVEPKLVVEIAFNEIQVSPRYPSGLALRFARVKRYRPDKS
                     AAEADTFQTVQEWAGLQAAEPPGDNAYGS"
     gene            complement(733..1203)
                     /locus_tag="VFQ79_04830"
     CDS             complement(733..1203)
                     /locus_tag="VFQ79_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139007.1"
                     /translation="MARFNWWAKTDLLPGSTAEFFVFETASLPGLTTAYVQSGVVPEA
                     PLNIPAGAAEALLEATKLENNSVRPLTIGPKIALSSEAQRRGVMRALAEEMRFAAAFS
                     EFREIQKVLLDLAQRTESGETGLDKAFSSAGASALQRAFSQAMQVSLQYAYQLP"
     gene            complement(1148..1609)
                     /locus_tag="VFQ79_04835"
     CDS             complement(1148..1609)
                     /locus_tag="VFQ79_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139008.1"
                     /translation="MIRDVAVLLALPFVHYGQSAHIIPGVPYDTMGAQNSGSTTVMPD
                     GVLLVSGTTSDGRPFKHQTWLANQVKPQVTASVEPAGAGTVRYQYSLSNQAGARDGIR
                     LFAVGMPQPDQLKNIGSPGSLSRERPFFSKLGRGGKIQLVGKNGSAAGIHR"
     gene            complement(1606..2208)
                     /locus_tag="VFQ79_04840"
     CDS             complement(1606..2208)
                     /locus_tag="VFQ79_04840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0139009.1"
                     /translation="MTLPGPRVADLPIARELRNTACELGFEMSCTPRMGALLQVLAAT
                     KPVGRLLELGTGVGVGTSYLAAGLAPSASLTTVELDGTLQAHARQLLSRPNIRFVTGD
                     AAEYITNCGQKFDLIFADTWAGKFTHLSETLELLNIGGLYVIDDLLPQPAWPDGHAPK
                     VPVLLNTLAGSEFLEGIEMDWDTGLALYVRLRREQEEDHS"
     gene            complement(2240..5404)
                     /locus_tag="VFQ79_04845"
     CDS             complement(2240..5404)
                     /locus_tag="VFQ79_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139010.1"
                     /translation="MSIKGRSLFLYLVGAACIGAQTIDTGILGTVSDSAGGRMAGAVV
                     TITQPQTGLTRSIQTDASGQFEVRYLVPGEYIVEVAATGFQRARRAGIQLQIGQQARL
                     EFTLQVGDVQQTVEVTGAAPLLQTENATLGEVVSHERIVNLPLNGRSFTQLAALTPGV
                     RVVDPSLYTASTDGSRIIANGARDIWMQVNLDGVTMVNNRSNYVNLYPSIDALQEFRV
                     QSGNYSAEYGGNGGANVNLQLRSGTNQFHGSVFEFLRNDAFDARGYFRPRPLSKDVLK
                     RNQFGAVVSGPILRDKTFFMAGYEAIRSERESPGTNIVLTPAQRGGDFSASAAPVVDP
                     LSGNPFPNNVIPQNRLNPVSVNLINTYMPLPNSAGTVNYNGVLRGTLKTDQGLTRLDH
                     YLSSKDQVFFHYIYSRRDFPNIDLNPNFRYNGTFPNSSLAVQHVHTFSPSLLNEVRFG
                     WNKANVSKLPPRANTDFTIESLGITGLQVGGPSGRPLRPDEQGFPVMNITGYLGMGDS
                     QASSNLDNSRTYQIVDNVSWIRGRHAVKFGGDIRRLLDDATTNNWPFGNMTFSGDLSG
                     NAAADFMLGYPRTTLTPEGVPISAVRQWRYGMYVQDDWKATSNLTLNLGLRYDLFGQP
                     REINGVTRTLRFDLDASGPVLWPELGEVVDVWKNEYNYIAPRFGFAYRLPQRMVLRGG
                     YGIFYSAAQFDNINILQLNPPTGGSLTVINPARNPVATIQNPVPAELYPENPIFNVVS
                     VPPDRKRRNAYLQNWNLQIARELSSNDVIEVGWVGSKGTHVDTSLNNFNQPDPGPGPI
                     QARRPYPQYARIRMIAPDTNTIYHSLQARFERRFHTGLSLTSAYTWSHMIDDAAQTIN
                     RGGCGCQDPRNRGRAERGDSIQDQRHRIVVGYVWELPFARSLRGVGGVFLSGWSTGGI
                     LTLASGFPFNVQQSGDTQNNDALWPRPHLVAGQRAKLDDPDPALWFNTAAFERSVLEY
                     GTAPRNPLVGPGTRTFDASISKDFRMPYAEAHRLLFRAELFNAFNTPQFSNPGGTLGT
                     GTFGRVTGTSQDNRQIQLALKYMF"
BASE COUNT         1076 a         1660 c         1714 g         1112 t
ORIGIN      
        1 aatcaccatg cggcggttcg ggcgcaaact ggatgttgag agaatgcgcg cggagcttcc
       61 gctccagccg ttctggttcg acctactgca tatcaatgga gccgacgtaa cggaagagcc
      121 gcaaacgcgc cggtttgcgg ccctccggga gattgcatcc gaggagaaca tcattccgcg
      181 gatcatgact gccgattccg ccgcggcccg cgatttcctg ctgctcgctc tgaagcacgg
      241 gcacgagggg atcatggcga aagcgctcag cgcaccgtac gccgcgggta agcgcgggca
      301 gggctggctc aaggtgaagc aggcgcgcac gctggacctg gtggtgctcg cggctgagtg
      361 ggggcatggc cgccgccgcg gctggctcag caatctgcac ctgggcgcgc gcgatacgaa
      421 acgcggtggc tacgccatgc tgggcaagac cttcaaagga ctcaccgatg cgatgctgtc
      481 atggcagact caggagttgc tgaaattcga aatcgcccgc gacgagtaca cggtgtacgt
      541 cgagcctaag ctggtggtgg agatcgcgtt caacgaaatc caggtcagcc cgcgataccc
      601 gagcgggctc gcgttgcgct ttgcacgtgt gaagcggtac cggccggaca agtccgccgc
      661 tgaagccgac acgtttcaaa ccgtgcagga gtgggccggt ctccaggcgg cggagccgcc
      721 tggagacaac gcttacggta gctgataggc gtattgcagg ctgacctgca ttgcctgtga
      781 aaacgctcgc tgtaacgccg atgcgcctgc gcttgaaaac gctttgtcca gtccggtttc
      841 tccagattcg gttctctgtg ccagatccag cagaaccttc tgaatctcgc ggaactcgga
      901 aaatgcggct gcgaaccgca tttcctcagc taatgctctc ataacgccgc ggcgttgcgc
      961 ctcagacgat agggcgatct tgggtccaat tgtgagaggc cggacactgt tattctcaag
     1021 ctttgttgct tcgagaagag cctctgcggc gcccgccggg atattcaagg gcgcctccgg
     1081 cactacaccc gattgaacgt acgccgtggt aagacccggc agcgacgcgg tttcgaagac
     1141 aaaaaattca gcggtggatc ccggcagcag atccgttttt gcccaccagt tgaatcttgc
     1201 caccccgccc cagcttgcta aagaagggcc gctcgcgcga aagggagccc ggcgatccga
     1261 tgttcttcaa ctggtcgggc tgaggcattc ctacggcaaa cagacgaatg ccatccctgg
     1321 cgccggcctg gttggaaagg ctgtactggt aacggacggt gcctgcaccc gctggctcga
     1381 cggacgctgt cacttgaggc ttaacttgat ttgcaagcca ggtttgatgc ttgaacggcc
     1441 tgccgtcaga tgtggtcccc gacaccaaaa ggacgccatc cggcatcacg gtggtgcttc
     1501 cggaattctg cgctcccatg gtgtcgtaag gcacacctgg gattatgtgt gcactctggc
     1561 cgtagtgcac gaaaggtaaa gccaggagca cggcaacatc gcgaatcatg agtgatcttc
     1621 ctcctgttct cgcctgagcc gaacgtagag cgcaagcccg gtatcccaat ccatttcgat
     1681 tccctcgagg aactcgctgc cggccaacgt attcaacaac acaggaactt tgggagcatg
     1741 tccgtcgggc cacgccggct gaggcagaag atcgtcgatg acgtaaaggc cgccgatatt
     1801 gagtagttcg agcgtctcgg agagatgcgt gaacttgccc gcccaggtat ccgcgaagat
     1861 cagatcgaat ttctgaccgc agtttgttat atattcggcg gcgtctccag tcacgaaccg
     1921 gatgtttggt ctggataaga gttgccgcgc gtgtgcttgc agtgtcccgt cgagctcgac
     1981 ggtcgtgagc gatgccgaag gcgccagccc tgccgccaga taacttgtcc caacccctac
     2041 cccggtgccc agctcaagca ggcggcccac cggcttggtc gccgcgagca cctgcaacag
     2101 cgcgcccatg cgcggcgtac aagacatctc gaatccgagt tcgcaggcgg tgttccggag
     2161 ctcccgtgca atcggcagat cagcgacacg ggggcctggc aatgtcatgc tttaccgtac
     2221 ttcatctaca gcttccggct caaaacatgt atttcaatgc cagttggatc tggcggttgt
     2281 cctgtgacgt gccggtaacc cggccgaagg tcccggttcc gagcgtgccg cccgggttcg
     2341 aaaactgtgg cgtgttgaac gcattgaaga gctcggcacg gaacagcagc cggtgtgcct
     2401 cggcgtaggg catgcggaag tctttcgaga tggaagcgtc gaacgtcctg gtgcccggcc
     2461 caaccagcgg attacgcgga gccgtaccgt actcgagcac gcttcgctca aacgccgccg
     2521 tgttgaacca tagggcggga tcggggtcgt ccagtttggc gcgctgtccg gctaccaggt
     2581 gtggccgcgg ccacagcgcg tcgttattct gcgtgtcgcc gctctgctgc acgttgaagg
     2641 gaaagccgct ggccagcgtc agaatgccgc cggtggacca gccgctcagg aacactccac
     2701 ctacgccgcg aaggctcctg gcgaatggca actcccaaac gtaaccgacg acaatacggt
     2761 ggcgttgatc ctggatgctg tcgccccgtt cagcgcgccc gcgattccgc ggatcctggc
     2821 agccgcagcc gccgcgatta atggtctgcg ccgcatcgtc gatcatgtgg gaccacgtat
     2881 aggcggaagt gaggctcaag cccgtgtgga agcggcgctc gaaccgagcc tgcaacgaat
     2941 ggtagatcgt attcgtatcg ggcgcgatca tgcggatgcg cgcatactgc gggtatggcc
     3001 gccgcgcctg aatcggcccc ggcccgggat cgggctggtt gaaattgttg agacttgtgt
     3061 ccacatgcgt ccctttgctc ccaacccagc cgacttcgat tacatcgttg gaactgagct
     3121 cgcgcgcgat ctgaaggttc cagttctgta gataggcgtt gcggcgcttg cggtcggggg
     3181 gcacgctcac gacgttgaaa atcggattct ccggatagag ttcggccggc acggggttct
     3241 gaatggtcgc gaccgggttc cgtgccgggt taatcaccgt caggctgccc ccggtcggcg
     3301 ggttcagttg caggatgttg atgttgtcga actgcgccgc cgaatagaag atgccgtagc
     3361 cgccgcggag caccattctt tgtggcaggc gataggcgaa gccgaagcgc ggtgcgatgt
     3421 agttgtattc gttcttccag acatccacca cttctccaag ctcgggccag agcaccggac
     3481 cgctcgcatc cagatcgaag cgcagagtac gcgtcacgcc attgatctcg cgcggctgcc
     3541 cgaacaggtc gtaacgcagc cccaggttga gtgtcagatt ggaggtcgcc ttccagtcgt
     3601 cctgaacgta catgccgtac cgccactggc gcaccgcgga aatcggcacg ccttcgggcg
     3661 tcagcgtggt tcgcggataa cccagcatga agtcggccgc agcattgccg ctcaggtctc
     3721 cagagaaggt catatttccg aacggccagt tattggtggt ggcatcgtcc agcagccggc
     3781 gaatatcgcc gccgaacttg acggcgtggc gcccgcggat ccaactgacg ttgtcgacga
     3841 tctggtacgt ccggctgttg tccagattcg atgacgcctg gctgtctccc atgccgaggt
     3901 agcctgtaat attcatcacc ggaaagcctt gttcgtcggg acgcagcggc cggccgctgg
     3961 ggcctccgac ttgcagcccg gtgattccca gcgactcgat ggtgaaatcc gtgttcgccc
     4021 ggggaggcag tttggagaca ttggccttgt tccagccgaa ccggacttcg ttcaatagcg
     4081 atgggctgaa cgtgtggacg tgttgtaccg ccaggctgct attggggaac gttccgttgt
     4141 agcgaaagtt cggattcaga tcgatgttcg ggaagtcgcg ccgcgagtaa atatagtgga
     4201 aaaacacctg gtccttggag ctgagatagt ggtccaggcg cgtaagtccc tggtctgtct
     4261 tcagcgttcc tctgagcaca ccgttgtagt tcaccgtacc cgccgaattc gggagaggca
     4321 tgtaggtgtt gatcaggttc accgacaccg ggttcaaccg gttttgaggg atgacgttat
     4381 tgggaaaagg atttccgctg agcggatcga cgaccggcgc ggcagacgcg gagaagtcgc
     4441 cgccgcgctg cgcgggcgtc aacacgatgt tggtgcccgg gctttcccgc tccgaccgga
     4501 tcgcctcgta cccggccatg aaaaaggtct tgtcgcgcag gatggggccg gagaccacgg
     4561 cgccgaactg gttgcgtttg agtacgtctt tcgacagtgg acgcgggcgg aaatagccgc
     4621 gcgcgtcgaa ggcgtcgttt cgcaggaact caaacacgct gccgtggaat tgatttgtgc
     4681 cggaacgcaa ttgcaggttc acattcgccc caccattgcc gccgtattcg gccgagtaat
     4741 tcccgctttg tacgcggaac tcctggagcg catcgatcga tggatagagg ttgacgtagt
     4801 tgctgcggtt attcaccatc gtcacgccgt ccaggttcac ctgcatccag atatcccgcg
     4861 caccgttggc gataattcgc gacccgtctg tgctggcggt atagagcgat gggtctacca
     4921 cgcgcacccc cggcgtcagt gcggccagtt gcgtaaaact ccgcccgttc aaaggcaggt
     4981 tcacaatgcg ttcgtggctc accacctcgc ccaaggtggc gttctcggtc tgaagcagcg
     5041 gagcggctcc cgttacttcc acggtctgtt gtacgtcgcc gacctgaagc gtaaactcca
     5101 ggcgcgcctg ttgcccgatc tgaagttgga taccggcgcg gcgtgctctc tgaaatcccg
     5161 tcgccgccac ctctacgatg tactcgcccg gtacaaggta gcgaacctcg aactggccgc
     5221 tggcgtcggt ctggatactg cgagtcagtc cggtctgcgg ctgggtgatg gttacgaccg
     5281 caccggccat ccggccaccg gcggaatccg atacggttcc cagaatccct gtgtcaatcg
     5341 tttgcgcccc gatgcaagcg gcaccaacga ggtaaagaaa gagactgcgc cctttgatag
     5401 acatgtgttc cccactttca tccccgcggg aatcccctcc caccgcacct ctgcccagat
     5461 tcctcccgcc agggaagctt ccatccaagg aacctatatc acaaccgccg ggagcgaaaa
     5521 agggcgggat gacggggaaa actgtaaaac cggtgcccaa ac
//
LOCUS       DASTMK010000118        28033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_166192, whole genome shotgun sequence.
ACCESSION   DASTMK010000118 DASTMK010000000
VERSION     DASTMK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 28033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28033
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166192"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFQ79_04850"
     CDS             complement(<1..874)
                     /locus_tag="VFQ79_04850"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU0139011.1"
                     /translation="MVRNWSSLTILTVLFPVALAQTPKHVIRPAEIHHVLVNPGMGIQ
                     TFQRFNGEALYPTLRWSEAGPVEPGKQASVKPDFPGTSLAYCRWFWSELEPQPGKYRW
                     EIIDNALEQARKHNQTLDIRIMPYDQGHPLPEWYRNSGARRANKPEDKDGAIWSPDAD
                     DPLYLKHWGALVRAAGARYDGHPYLDSVDVSTVGYWGEGWGPYLPSWQTQKELIDLYF
                     EAFPTTILLMNFDEPRALAYGVKKGAGWRLDCWGDMGGRGRGMMHMLDMYPQQVVRTD
                     IEDAWRKYPVSLETC"
     gene            1006..1881
                     /locus_tag="VFQ79_04855"
     CDS             1006..1881
                     /locus_tag="VFQ79_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139012.1"
                     /translation="MTDSTPTYSHQPGGSGLKIPILFGVVIALVAANVYLFLQVDRLH
                     ADVAKMRESILTEITNLRESSTVTTQANRRNVETLRDELEAARRQASMAAGQAKTEAT
                     KRAEDLARRLEIEAKRQQEQVSSALTEVKQANEETAQKVGAVSTEVGAVKTEVAATKS
                     ELDQTLSNLKRVFGELDSHASLIATNGKELDALRALGDRNYFEFRIRKAKQPQKVGDI
                     GVKVKKTDTKRNKYTIELLADDKTVEKKDRTINEPVQFYVAKARQPYELVVNQVNKDE
                     IVGYLATPKLQSARK"
     gene            complement(1893..3968)
                     /locus_tag="VFQ79_04860"
     CDS             complement(1893..3968)
                     /locus_tag="VFQ79_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0139013.1"
                     /translation="MSGHLLSGVRSRPVSYALAVVLSLAAIVVDAQLSPEDLPEATLA
                     LLAAIVLSVWVGGKGPGLVSVALSCCYLAYAVIEPRGSIQVGGFKGAFELVFFGVISF
                     LLVWFVGRLRETKELLAAMFSSVGDAMIVADSRRRILLMNARAEEWTGVPQHVAGRKR
                     LIDVWPAAAELAKGRFESKGRTFEKFEIPLLKYGVLYVFRDITERLKWEEQRLLVDRL
                     ISGMKDGFNVVDLEDGKILRTNEAFDRMFGYETGELIGRPISVVNAGGPESQRQIAES
                     IWAEVTRSGFWEGEVLNKRKDGSEFLTFANITLHKERGRSYLTTIQQNITERKRAEEA
                     LREAKDVLQAVIETSPLAKIVFDLEGRVALWNPAAERLFGWSAAEALGRFNPILPEDH
                     VAGESALELAETPGPPSGHETRRRRKDGSIIDVALWTAPLRDHSGAMRGVLAIFADIT
                     ERRRLENSIQERTAELMRSNEDLQQFAFIISHDLQEPLRIVSSYAQLVAKRYPDALDE
                     EGHEFLRHITEGVSRMRQLIQDLLAYSRASQGGGLQMEETDVQGVVAWALMNLQVSIQ
                     ESGAHVTYEKLPAVYGDRAQLAQLFQNLIGNAIKYRSAAPPRIHIQAESRKGEWMFSV
                     QDNGIGIAPEYRERVFGIFKRLHGREYPGTGIGLAICKRIVERHGGRIWVESEDGAGS
                     TFCFTIAAD"
     gene            complement(4017..4745)
                     /locus_tag="VFQ79_04865"
     CDS             complement(4017..4745)
                     /locus_tag="VFQ79_04865"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEU0139014.1"
                     /translation="MDSLSIVIPAYNEANRLPASLDAVLAYAEGRFPRCEVIVVDDGS
                     TDKTAETVEALGDARVRLLRNPGNRGKGYSVRHGMLAATGDWRLFTDADLSAPIDELG
                     RLFAEAQRTGAAVAIGSRAIDRRLISVRQSWIRETAGRIFNIAMRAVTGLDFEDTQCG
                     FKLFTAEAAQRIFKRQLLDGFGFDVEDLYIAKKLGIKVVEVPVRWAHAEGTKVGLASG
                     LKAFFDPLRIRWNNLKGLYNSEIG"
     gene            4857..6362
                     /gene="lysS"
                     /locus_tag="VFQ79_04870"
     CDS             4857..6362
                     /gene="lysS"
                     /locus_tag="VFQ79_04870"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU0139015.1"
                     /translation="MSLETELLKQRAQRIHQIQELGYEPYGRRFEFSHTIPEILHDYG
                     SKSADELADPPVRVRLCGRVETIRRMGKAGFAHLMQRGERLQIYIRKDAVPPSDFELY
                     QLVDLGDIIGAEGYLFRTRTGELSLHVEKLDFLSKTLLTMPEKWHGLEDVETRYRQRY
                     LDLIANPEVQEIFRKRSKIIASLRRQLDTRGFIEVETPMMQPLYGGAIARPFVTHHNT
                     LDLDLYLRIAPELYLKRLIVGGLERVYEINRNFRNEGISTQHNPEFTMLEFYQAYTDY
                     RGLMDLSEELLRQTALDVNGTALIEYQGTQLDFDHIRRFSMREAVLEFWSGADKPSAA
                     DLADPDWLGRHSTKLTSGEALAEIFERVCEPHLIQPTIIYDYPVETSPLSKNKPGNPE
                     FVERFEIYAAGMELGNAYTELNDPQEQRRRFEMQIAMRERGDEEAHQMDEDYVRALSY
                     GMPPTGGEGIGIDRLTMILTNSRSIRDVILFPLLRPEGPIGLLEQLRELDA"
     gene            6369..7610
                     /locus_tag="VFQ79_04875"
     CDS             6369..7610
                     /locus_tag="VFQ79_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139016.1"
                     /translation="MGKFNGFEAFIARRYLRAKRKQAVISVITAISIVGVAAGVMALV
                     IALAVNNGFRNTLQRNLLGATAHVSVMEKEPSYGIQDWREITERLRKLPHVVAVAPAL
                     YGNVFLAGPVRSDGAVLKGVDVDSELGIASVLRNLKEGSIDPLRHAGDEMYLVLGSGL
                     ARETGMLTGGRVQVISPQGELTPFGPRPSYYRMRVAGIFESNFYELDMRWAYTSLASA
                     QHVLSLPDVVNAIELKLDDIYRAPEVARVAEEAAGPKYAATNWQEQNRQLLNALKMER
                     AVTIITIGLIELVAALNILITLIMMVMEKYKDIAILMSMGARKEQIRKIFMFQGVMIG
                     VVGTVIGLIAGYGLSFLANHYRLVPLEASVYSMSFVPFEPRWVDGLWIAGLAIFVSFI
                     ATIYPARSASRIAPAEAVRYE"
     gene            7603..9384
                     /locus_tag="VFQ79_04880"
     CDS             7603..9384
                     /locus_tag="VFQ79_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0139017.1"
                     /translation="MSERPRAYEILVVAVAAIIFLGTAFSPPHLMDDVDAVQAQISRN
                     MLESGDWVTARLDGVKYLEKPPLKYWMIAVSYMIFGIHDWAARLPIALSAVTLCWVVF
                     RFGRWAFSPRAGFCAALALATCIGLFLFTRVLIADVILTLAVAVSLYGFLRCLDEQER
                     NTRLWSAVFAGGIGCGLLLKGLIAAVFPIAAAGLFLLFTKELFARRTWRRLHPFTGLL
                     VIFAIAAPWHLLAIIRNPPYFDFTMHSGPGQYRGFFWFYFLNEHVFRFLNMRYPRDYN
                     TVPLVYFWLLHLVWLFPWSAFLPGTLKLGYKPVDRAGRTRLLALCWIGFVLVFFSFST
                     TQEYYSMPAYPAFALLLGCAVAADTAWLRAGLKVLVIVCSLAAAACAAILVQVWNLPS
                     PGDISNALTQNPDAYTLSMGHMGDLTLASFAYLRPPLALAAVAFLIGAAAAFFWKSHR
                     AYLGVALMMTLFTHAARVAMIQFDPYLSSWHLAEALRSSPPGKLIAEDQYYTFSSVFF
                     YAGRDALLLNGRVNNLEYGSNAPGAPDVFLGDDDLKRLWHGSGSWYLVARKQAVPHLE
                     SVVGPGALNVVKESGGKLLFSNRAAVH"
     gene            9409..9687
                     /locus_tag="VFQ79_04885"
     CDS             9409..9687
                     /locus_tag="VFQ79_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139018.1"
                     /translation="MDERAFYTESQTTKPARLSCPWCKTAETYQLRWLLRKKKDRLPG
                     GGGERDRARFQKAQSYMVLMEDKVMCKNMRCRKRFDVSGIKSMAFLTE"
     gene            9684..10283
                     /locus_tag="VFQ79_04890"
     CDS             9684..10283
                     /locus_tag="VFQ79_04890"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEU0139019.1"
                     /translation="MISKRTGLFLFLALAVGAGVLLMFLLWSVWPFRGGFQRFIDAPV
                     IVREVQQLSELVSVKYSVQKVVGLKEDKIPFGTESILLVVEGRVLGGINLGALREQDV
                     EVRGRKVWVRLPPPEILHVYLNEEQTKVWDRRITWWTPWVPFSPDLEQRARLAALESI
                     KESALEMGLYAEARKSAETAIRGLLRAMGAESVEFAPSS"
     gene            complement(10287..11537)
                     /locus_tag="VFQ79_04895"
     CDS             complement(10287..11537)
                     /locus_tag="VFQ79_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0139020.1"
                     /translation="MNRRDLFRFAPAALIPSAALSQAPLAGEKSGLKITSVRLVGMRP
                     RRPVPSYTPAPGSWSTGGVEVANPMSIYPEYKASRALFLPDPGKLGGFWVEIATDKGV
                     KGYGTGGPGGGYVVEQHLTKLLIGEDPFNLERIWDILWRSTMSYGRAGIAINGISGVD
                     IALWDLVGKAVGLPVYKLLGGQTKARIPAYCTGNDIEQHVEFGYTKLKLAIPHGPADG
                     REGQRTNVELVKRARTALGPDGEIMLDCWMAWDERYTLEMADLVAPYRVYWMEECLQP
                     HDYEGFGRLNAQIKSTRIVTGEHEYTRYGFRQLLEHQAAAIWQPDIHWCGGLTELRRI
                     GALAAAYDIPVIPHGGGSRDSVHFIMASVNSPWAEMFMPAPGGPPEVYKRYEEDNLLT
                     RGPEGIYTRPAGRPGFGWDLEAVS"
     gene            11641..12672
                     /locus_tag="VFQ79_04900"
     CDS             11641..12672
                     /locus_tag="VFQ79_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU0139021.1"
                     /translation="MSKLVVTFGEIMLRLAPPGFERFLQSPQFVATFGGGEANVAVAL
                     ANFGNPARYVTVLPSNPIADAFVGELRRFGVDASAIVRSKGRMGIYFVEPGANQRPSK
                     VVYDRENSAIALAKPGDVDWNKTFDGASWFHVTGITPAISQGAADLALESVRVAREKG
                     LTVSCDLNYRKNLWKYGKTAKEVMSELFKSVDVGVANEEDCQMSLGIQADVDVHSGKL
                     DGEQYRKLTDKVLAQYPNLKMLGVTLRESFSASHNGWSACLNDREQFIVSRRYDITHI
                     VDRVGGGDSFAGGLIYGLLNLSSHQEALEFAVAASCLKHSIPGDFNRFTVDEVKGLLK
                     GGGSGRVQR"
     gene            12693..13691
                     /gene="pta"
                     /locus_tag="VFQ79_04905"
     CDS             12693..13691
                     /gene="pta"
                     /locus_tag="VFQ79_04905"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU0139022.1"
                     /translation="MALPESAVTFLSRHIERIQRLARKKRIVFPEGNDPRVIAAAVRL
                     ERDGVVEPILIGPKPAGVSGVASIDPADSPLVRKYAAVLYEKRRARGVTESEAAEFAR
                     RPLYFAALMVAAGDADGGVGGAATSTADTVRAALHSIGMPPRFKTVSSMSILASRESG
                     LGHHGLLAFADCAIVIEPSAVQLAEIAIASAESTRVLIGVEPAVALLSFSTKGSAKHK
                     QVDKIIEALRIVKARAPDLHVDGELQADAALAEAVGRAKAPGSTVAGHANTLVFPDLN
                     SANIGCKIAERIGGVAVMGPFLQGLEKPFNDLSRGCSVDDIYNTAVVTAVQTQTYY"
     gene            13705..15261
                     /gene="garD"
                     /locus_tag="VFQ79_04910"
     CDS             13705..15261
                     /gene="garD"
                     /locus_tag="VFQ79_04910"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HEU0139023.1"
                     /translation="MDPLYVRVHPRDNVAIIVNSEGLPSGTEFPDGLRLKDSIPQAHK
                     VALHEIGTAEPIVRYGQNIGFANRPIPAGAWVREEMLDLPAAPALDALPLATAVPPPL
                     PELAGYTFEGYRNAGGTAGTRNILGITTTVQCVAPTVEHAVKRIRSEILPHFPGVDDV
                     AAITHSYGCGVAIDAPGAAVPIRTLRNIVRNPNFGAAPLVVSLGCEKLQPSRLLPDQR
                     LPSASVSELPVLEDEPYVIRLQDSELHGFGDMVAAIMRFARKRLEELDRRRRVECPAS
                     DLVVGLQCGGSDAFSGVTCNPALGFAADLLVRAGATVMFSEVTEVRDAVHLLTPRAVN
                     EQVARDLIREMRWYDEYLAGGLADRSANPTPGNKRGGLSNVVEKALGSIAKAGSSALM
                     AVAGHGERVTAKGLVFAATPASDFICGTQQLTSMNMHVFTTGEGSPYGLALVPVVKVS
                     SRTALAERWHDLIDVDAGRIATGNASIESVGWEIFRLILDVASGRKRTWAEKWGLHNA
                     LSPFNPGPVT"
     gene            15327..18788
                     /locus_tag="VFQ79_04915"
     CDS             15327..18788
                     /locus_tag="VFQ79_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139024.1"
                     /translation="MKGTLIWAISLAVLAGTLLGQDPRGGITGRVVDSSGAIVPGVRI
                     RATNNETGVSAPATSGPAGTFTIPYLLPGFYRVTAELPGFKSFVRDKVEIRVSELVDL
                     TINMEVGDVTETVEVSAESPLLDTANSSLGQVMDQRRIAELPQRGANPLELALLTPGV
                     VNATNLRLRKAMAPEATSQIAADGAGTYNNEFQIDGISNVSADRGSGYGRVAFSPPQS
                     AVREFKMQTSAYDASVGNTMGALLNVSTASGTNEYHGEAHYFLRHSALDAPNFFANKN
                     NQRQPVYQDHRYGGSIGGPLIIPKLYNGRNRTFWFYTAEQNPFGVPVQFTRTVPTVAQ
                     RMGDFSALLAVNPTTYQIYDPLTTTPAANGRFQRLPFAGNIIPQNRLDPTGLALVNLY
                     PMPNVPGTNDGRNNFFIAGKAFQDYYVHLVRLDHAFSDTHRAFLRVHYDMWREQKNND
                     FGTWINGIHQNRPNRGIAVDDVIVLNQSTVLNLRYGMTSTKWWQYRVTRGYDLASLGF
                     SPNLLRYLDPGQSPIPRISAGAYSQISWWENPGDGVNSSLTHMFLANVNRLQGRHNIK
                     FGSDFRVFRSFNNRRPIGVAPDFIFGTTYTRGPLDTSAAAPIGQDLASMLLGIPDGEM
                     QIVASSALQHKYLGLYIHDDVRLTRKLTVNLGLRYELERPFTERYNRLVADFDSGSSS
                     PIEAEARANYALNPIPELPVDAFRVRGGLSWVDENRRSPYRGETNNFMPRIGFAYQAT
                     NSTVLRGGYGLFYDSIGIMKVVPIQTGFTQTTPIQASLDNGQTYFATTSNPFPNGLIV
                     PQGPEGGLRTSLGQNLDFYNPDLKHGYSQRWSFGVQQTLPAQFLIDASYVANRATRLG
                     VDRQINVTPAQYLSTSPTRDQAHINYLSQQFPNPFRGLDPIYGANISRANLLRPYPQF
                     GNITVEEPIGYSWYHSLQVRGERRFAQGFTFQLAYTFSRLMEATQFLNTVDPMPYEIV
                     SSLDRPHRLAASGIWEVPVGRGRRFGSGMHRALDYAIGGWQIGGILTKQSGPPLDFGN
                     IIFNGDPDNIKLPSGQRDVDRWFNVDAGFNRNAQQQLASNLRTFPLRFSGLRGGGQNS
                     WDFSIVKNFPLHEQARAQFRADVYNAWNQTNLNTPNMAPTNTAFGRVTSTTGDARNWQ
                     LSLKVMF"
     gene            18835..19908
                     /locus_tag="VFQ79_04920"
     CDS             18835..19908
                     /locus_tag="VFQ79_04920"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEU0139025.1"
                     /translation="MEEVRVTSWAHLDEQLYAESWFQPHGRFRSPYVFRGVAAAGSDL
                     KTCLMRLGGHYGELEDDLLRNFRKYAHREAAPGGSWWNWLAVAQHHGLPTRLLDWTFS
                     PFVALHFATADFSLFAADAVVWAVDCIAVQEFLPEKLREILKQEGATVFSAEMLDRYA
                     GNLADFDRRVREEQGECVVFFEPPSLDERIVNQMALFSMMSSAEADLWTWLARREQKS
                     KRKLYRKIVIPAPLKWEIRDKLDMANVNERVMFPGLDGLSAWLRRYYSPNNIIEITYF
                     ESDGQPHTYPAVITSISNAMMEVAYHCEREGRRTARLFTREGSIWWDECRDSAVAVNP
                     EPAAEKTFPGNLPGAHQAGPAQV"
     gene            19938..20771
                     /locus_tag="VFQ79_04925"
     CDS             19938..20771
                     /locus_tag="VFQ79_04925"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0139026.1"
                     /translation="MDKRRMCPNCRAFITTSDKVCPYCDTPVGPRAVDRRKSAEISAG
                     FIPHARFVTVLILTINFGLYAATALFSMQNSGASFMDIDGRTLLLFGAKYNEFIAAGQ
                     WWRLITAGFLHGGLFHILMNSWVLFDLGAQVEELYGARRLIVIYFLATVFGFMVSTWW
                     SPSLSIGASAGIFGLLGAMIALGMRSGTAMGAAIRGLYIRWAVYGLLIGLLPFFRIDN
                     AAHIGGLIAGFVTGFVAGTPLPRRSVPEKLWGLAALACLLMTAFAFLQMFLFFGVASR
                     F"
     gene            complement(20768..22963)
                     /locus_tag="VFQ79_04930"
     CDS             complement(20768..22963)
                     /locus_tag="VFQ79_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0139027.1"
                     /translation="MKTYLAYTKITLKLTFRDRVVIFFNYVFPLLFFFMFGQLSEAAQ
                     GAAIYQVVSMVLIIGVLGNGFFGAGVRAVQDREFNILRRYKVTPITATPVLVSSLITG
                     WVNYFPSGLMILVLAHLIYGMPWPERWPELLVFLSIGVFAFRAMGLIIASVVNSLQES
                     QIIIQLFYMPMLILSLFPLTSMPHWLQAIAQFLPSPYLFMGMQSILVRREGLAANIVP
                     IVALLVTTLLATFIGVKLFRWEKEEKVRRSSKLWLAAILAPFLLLGAWQFYSRDNIVK
                     ARILERQMYRDRTWLIRNVRVFAGDGTVIPAATVLIRGGRIDTLYPSDPSGAGVKAET
                     IEGAGKTLLPGLIDTHVHLAVPGGVYEEPGEYEPHKAIRRALAAYLYSGVTVVRSVGD
                     PLELVRGAGGQIASGERLGAEVFTTGPVFTAPEGYGTEYFDTVPQPFRDALTRTVVRT
                     PASVEQARQEVKDLAGQGVQAIKAILEAGASGRVFPRLDTSILKAIVVEAHAHSLPAV
                     VHTGDSRDVREAVEAGADAIAHGSTRDEIPDEIWNAMARRKVMFVPALVAVEALQQIA
                     QGSEALLERSLAQQVGPAPLLHATHDFLVSGQAAPLREKVSLLQPNLKLAMTNLQRAY
                     KAGVPLVAGSDAGNLLVTHGPAVHRELQLWVEAGVPARDALTAATGNAAAALRASDRL
                     GLIRPGCEASMLLVDGNPLEEIAATERISAVFFRGERLDRASLFKQGDN"
     gene            complement(22960..23895)
                     /locus_tag="VFQ79_04935"
     CDS             complement(22960..23895)
                     /locus_tag="VFQ79_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139028.1"
                     /translation="MAQSQVAPIKVCGLRKSYGAFEAIRGIDFEVAEGEVFGLLGPNG
                     AGKTTTVEILEGLRTRTAGDVSVLGCDPAVQVRALKDRIGVCLQATNLPEKITVREAV
                     DLFSAFYSRAADATRLLERLQLWQKRDEYYVHLSGGQKQRLALALSMVNDPQLLFLDE
                     PTTGLDPQVRLEIHSLIQQLKHAGCTVLLTTHYIEEAEKLCDRVAIVDGGKIIAIGTP
                     REIQSKTVGDSLIEMRCETPVAEGVMPPVAGAQRISLSKDRMMLTATSTHPTRTLVEL
                     VKWVDQQGIELADIHLKRPTLEDVFIELTGKSLRD"
     gene            complement(23910..24446)
                     /locus_tag="VFQ79_04940"
     CDS             complement(23910..24446)
                     /locus_tag="VFQ79_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEU0139029.1"
                     /translation="MELKGKRIAVLVDNLYQEMEVWYPYFRFKEAGAEVVAVGARAGT
                     TYTSKLGYPIVCDVSYDEVKAQDYDGVVIPGGFAPDHIRRHERANQFVKEMDDQGKLV
                     AAICHGLWVPCSARILKGRRVTCFFAIKDDVIHAGGLYEDSEVVVDNNLVSSRKPEDL
                     PAFCRSAIEVLSKAPVAV"
     gene            complement(24545..24820)
                     /locus_tag="VFQ79_04945"
     CDS             complement(24545..24820)
                     /locus_tag="VFQ79_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139030.1"
                     /translation="MSEQPPLKPLHPASSLSPAKLAVFEKFTTEKLKASLAPGQEHCL
                     KARPDGTILDGHHQVHVLKGRAENVDALPREVIRQSQYEIEREKYMK"
     gene            complement(24817..25320)
                     /locus_tag="VFQ79_04950"
     CDS             complement(24817..25320)
                     /locus_tag="VFQ79_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEU0139031.1"
                     /translation="MMWTKLHWVDGPWPGKLALAARPRGGEWLEDEITSWQREGVNTV
                     FSLLTDEEERDLGILKEKAEVEAHGMKFLSYPIPDRQVPDSEANLGKALAKLEAELAA
                     GRNVVLHCRQGIGRTGLVAACLLLTKGIDPESAITRLSAARGTSIPETPEQRRWIDHY
                     AESFAPR"
     gene            complement(25403..>28033)
                     /locus_tag="VFQ79_04955"
     CDS             complement(25403..>28033)
                     /locus_tag="VFQ79_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU0139032.1"
                     /translation="SMEDVLIALEMGEVETLTPIKLRYTGRVIDLSKAFDNQNVMHTE
                     PIEFNKQYMDTTVGRVILNDVLPEDMPFINGLLKKKGLSQLVHYCYLRFGLQTTVRML
                     DEIKSLGFLYATRAGISIGVDDMVVPGSKAALVKDAQKQVIEVQQQYQEGAITQGERY
                     NKIIEIWSKVTERVSEEMFRVMEEDDRTGRYLNPIYIMADSGARGSKQQIRQLSGMRG
                     LMARPSGEIIETPITANFREGLNVLQYFISTHGARKGLADTALKTADSGYLTRRLVDV
                     AQDVIVTELDCGTGDGIYVEPIIESGEIIEPLRDRIVGRVALEDQLDYEGNVIVNVNH
                     EITEELAGAIQAAGIERVKIRSVLTCESKRGVCQLCYGRNLATGRMVERGEAVGVIAA
                     QSIGEPGTQLTMRTFHIGGAATRVSEQSTQDAKSDGFVKYIGISTVRNARGELIAMNR
                     TGILAIVDDKQREKERYPVVYGAKVLVGDGDPVKANQILLEWDPYTFSILTEVSGVVH
                     FKDLQEGLTVREQVDEITGMSQWVVSDSPDEKRLPSVIVRSGTSRADEKRYLMPTHAH
                     LMVREGDEIFAGDVVAKIPRATTKTKDITGGLPRVVELFEARKPRETAVIAEINGTVK
                     YGDVSKGTRKIYIMGDDGQEREYSLPRGVHINVQESERVRAGDPLMDGPRNPHDILHV
                     LGEKELQKYLVNEIQEVYRLQGVNINDKHLEVIVRQMMRWVKIDDIGDTEFLPEEVVD
                     RFKFREENQRVMDAGGRPAQGRPVLLGITKASLSTDSFISAASFQETTRVLTEAAING
                     KVDNLRGLKENVIMGRLIPAGTGMEYYRTVKIAGEDIEEEPITEPETVMEGIPGYEEE
                     TRLTFTGGLSEDSGEGSLAE"
BASE COUNT         5565 a         8686 c         8115 g         5667 t
ORIGIN      
        1 cgcacgtttc aagcgaaacg ggatatttcc gccaggcatc ctcaatgtct gtgcgtacca
       61 cctgttgcgg gtacatatcg agcatgtgca tcatgccccg gccgcgcccg cccatgtctc
      121 cccaacaatc caaacgccaa cctgcgccct tcttcacgcc ataggccagc gcgcgtggct
      181 catcgaagtt catcaggaga atggtcgttg ggaacgcttc gaaatagagg tcgatcaatt
      241 ctttctgcgt ttgccagctc ggaagataag gaccccaacc ttctccccaa taacccacag
      301 ttgaaacgtc gaccgaatcg agataggggt gtccgtcgta gcgcgcgccg gcggcacgaa
      361 ccagtgctcc ccagtgtttg agatacagag gatcgtccgc gtccggcgac cagatcgcgc
      421 cgtctttatc ctccggcttg ttcgcccgcc gggcgccgga gttgcggtac cactccggaa
      481 gaggatggcc ctggtcatag ggcatgattc tgatgtcgag cgtctggttg tgcttgcgcg
      541 cctgctccag ggcattgtcg atgatctccc accggtactt gccgggctgc ggttcgagct
      601 cagaccagaa ccagcggcaa taggccagcg aagtgccggg aaaatccggc ttcacggacg
      661 cctgcttgcc gggttcaacc ggcccggctt cggaccatcg aagggtcgga tataacgctt
      721 cgccgttgaa gcgttgaaag gtttgtatgc ccatgccggg attgacgagc acgtggtgaa
      781 tttccgccgg ccggatgaca tgcttcggtg tctgggcgag ggcgacagga aaaagtacgg
      841 ttaaaatagt aagtgacgac caattacgga ccatgctcag gttctatcac aacgcggacg
      901 gccggtcaca aaaccccatg tccccgctgg tggtattcac ccgacgggca taggtgctaa
      961 agtaaacctg aaggtggcac agccgacacg aggagggaac attttgtgac cgacagcacc
     1021 ccgacgtact ctcatcagcc aggtggatcg ggcttgaaga tcccaatcct gttcggagtt
     1081 gtaatcgctc tggtggcagc caacgtttat ctgtttttgc aggtcgaccg tctccatgcg
     1141 gacgttgcaa aaatgcgaga gtccattctg acggagatca cgaatcttcg cgaatcgtcc
     1201 accgttacaa cacaggcgaa tcggcggaat gtggagacac tccgcgatga attggaagct
     1261 gcgcgccggc aggcgtccat ggccgcgggg caggctaaga ccgaggccac gaagcgtgcg
     1321 gaagacctgg cacgccggct ggaaatagag gcgaaacgcc agcaggagca ggtctcaagc
     1381 gctctcacgg aagtgaagca agccaacgaa gagactgcgc aaaaagtggg tgccgtcagc
     1441 acagaagtgg gcgccgtaaa aaccgaggtt gccgctacga aatcggaact cgaccagacc
     1501 ctttcaaacc tgaagcgggt atttggcgaa ctggatagtc acgcctccct gattgcgacg
     1561 aacggaaaag agctggatgc cctgcgtgcc ctcggtgacc ggaattactt cgagttccgt
     1621 atccgcaaag ccaagcagcc gcagaaggta ggcgacattg gcgtaaaggt gaagaaaacc
     1681 gatacgaagc ggaacaagta cacgatcgag cttctggcgg acgacaagac ggtggaaaag
     1741 aaggacagaa ccatcaatga accggtgcag ttctacgtcg ccaaagcacg gcagccctac
     1801 gaactcgtcg tgaatcaggt aaacaaggat gagatcgtgg gctaccttgc gacgcctaag
     1861 cttcaatcgg cccgcaagta acagcgccct gctcaatcag ccgcaatcgt gaagcaaaac
     1921 gtggaaccgg ctccatcctc cgattccacc cagatacggc ctccgtgccg ttcgacgatc
     1981 cgcttgcaga ttgcaaggcc aatgccggta cccgggtatt cgcgcccgtg cagccgtttg
     2041 aagataccga acacccgctc gcggtactcc ggggcgatcc cgatcccgtt gtcctgaact
     2101 gaaaacatcc attcgccttt tctgctttcg gcctggatgt ggattcgtgg aggagcggca
     2161 ctgcgatatt tgatcgcatt gccgatcaga ttctgaaaaa gctgagcaag ctgcgcccgg
     2221 tcgccatata cggcaggtag tttctcgtaa gtgacgtgcg cgccgctctc ctgaatggag
     2281 acctgaaggt tcatcagcgc ccaggcaact acgccctgca catcggtctc ttccatctgt
     2341 aatccgccgc cctggctcgc acgggagtaa gccaacagat cctgaatgag ttgccgcatg
     2401 cggctcacac cctctgtgat gtgccgcagg aattcgtggc cttcctcgtc aagtgcgtcc
     2461 ggataccgct tggccaccag ttgagcgtag ctggagacga tgcgaagagg ctcctgcaga
     2521 tcatgcgaaa tgataaacgc gaactgttgc aggtcctcgt tagagcgcat cagctccgca
     2581 gtgcgttcct ggatgctgtt ttccagcctc cggcgctccg tgatgtcggc gaaaatggct
     2641 aacaccccac gcatcgcgcc ggagtggtct cgcaaaggcg cggtccataa cgctacgtcg
     2701 atgatactgc cgtctttgcg ccggcggcgg gtttcatgtc ccgacggggg gcctggcgtt
     2761 tcggcaagct cgagcgccga ttcgcccgca acatgatctt caggcaggat cggattaaaa
     2821 cggccaagcg cttccgcggc agaccagccg aacaatcgct ccgcggcggg attccacaac
     2881 gcgacccgtc cttcaagatc gaaaacaatc tttgccagcg gtgaggtttc gatcaccgcc
     2941 tgaagcacat ctttcgcttc gcgaagcgcc tcttccgcgc gcttcctctc ggtaatattc
     3001 tgctggatgg tggtcagata actgcgcccg cgttctttat ggagggtgat gttggcaaag
     3061 gtcaggaact ccgagccgtc cttgcgcttg ttcaatactt cgccttccca gaagccgctg
     3121 cgcgtgacct ccgcccagat cgattcggca atttgacgct gactctccgg ccctccggcg
     3181 tttacgacgc ttatgggccg gccgatgagt tctcccgtct cgtatccgaa catccggtcg
     3241 aacgcctcat tggtgcgcag gatttttccg tcttcgaggt cgacgacgtt gaacccatcc
     3301 ttcatgccgg aaatcagccg gtctaccaga agccgctgtt cttcccactt cagccgctcg
     3361 gtgatgtcac ggaaaacgta gagcacgcca tatttaagca gcggaatctc aaacttttca
     3421 aaggtacggc ccttgctctc gaatctgccc tttgccagct ccgccgccgc gggccatacg
     3481 tcaatcagcc tcttcctgcc ggcaacatgc tggggcactc ctgtccactc ctccgccctg
     3541 gcgttcatca gcaggatgcg gcgccggctg tcggccacga tcatcgcgtc cccgacgctc
     3601 gaaaacatcg cggcgagcag ttctttcgtc tcccgcagtc ttccgacaaa ccagacgagc
     3661 aggaacgaga taacgccgaa aaagacgagt tcgaaagcgc ccttgaagcc ccccacctga
     3721 atagagccgc ggggctcgat tacggcatat gcgagatagc agcacgagag tgctacgctc
     3781 accaggcccg gccccttgcc tcctacccag acgcttagaa caatcgcggc gagcaaggca
     3841 agtgtcgcct ccggcaaatc ttcgggcgaa agctgggcat caacgacgat tgcggcgagc
     3901 gacagcacga cggccagtgc gtacgaaaca ggtcgtgaac gaacgccact aagaagatga
     3961 ccgctcatag ttcccgagtt tctctcgcac caggatctcg taccgactga gtgattctat
     4021 ccgatttcag aattgtacaa gccctttaag ttgttccagc ggatgcggag aggatcgaag
     4081 aacgctttca aaccggaggc caagcctacc ttcgtgcctt ccgcatgcgc ccaccggacc
     4141 ggcacttcca ctaccttgat accaagcttc ttggcaatgt ataggtcttc cacatcgaat
     4201 ccgaaaccgt ccagcaactg gcgcttgaaa atcctctgag ccgcttccgc ggtgaatagt
     4261 ttaaacccgc actgcgtatc ctcgaaatcc aggcccgtca cggcgcgcat ggcgatgttg
     4321 aatatccgtc ccgccgtctc acgtatccag gactggcgaa ccgagatcag ccgtctgtca
     4381 attgcacggg aaccaatggc aactgccgcc ccggtgcgct gcgcttcagc aaatagccgc
     4441 cccagttcgt caatgggtgc cgacaagtct gcgtccgtaa acagccgcca atcaccagtc
     4501 gcggccagca tgccatgccg gacagagtag ccctttcccc ggttgccggg atttcggagg
     4561 agccggactc gcgcgtcgcc caacgcctct accgtctccg cagttttatc cgtggagccg
     4621 tcatcgacaa cgatcacctc gcaccgtgga aatctcccct cggcataagc aagaaccgcg
     4681 tccagggagg caggaagacg atttgcttca ttataggcgg gaataacaat cgagagcgaa
     4741 tccaatcgac aattataata ggagattcag ggccattgga caaattgcgt ttccgggcgg
     4801 ccctgtccag ccataccagc cagcatcgaa ccgttcttga ttcgagagga gacgaattgt
     4861 cgcttgaaac cgagctactg aagcagcgcg cccaacgcat ccaccagatc caggaactgg
     4921 gttacgagcc ctacgggcgc cgcttcgagt ttagccatac cattccggag attctgcacg
     4981 actatggatc gaaatccgcc gacgaactcg cggacccgcc ggtccgggtt cgcctctgcg
     5041 gtcgggtcga gaccatccgg agaatgggaa aggcggggtt tgcgcacctg atgcagcgcg
     5101 gcgaacgtct ccagatctac atcaggaaag atgccgtacc tccctccgac ttcgagcttt
     5161 atcagttggt cgatctcggc gacatcatcg gtgcggaagg ctacctgttt cgtacccgca
     5221 ccggtgagtt gagcctgcac gtggaaaagc tcgacttcct ctcgaagaca ctgctcacca
     5281 tgccggagaa gtggcacggt ttggaagacg tggagacccg ctaccggcag aggtacctgg
     5341 acctgatcgc gaatcccgaa gtacaggaga ttttcaggaa acgcagcaag atcatcgcgt
     5401 ccctacgccg gcagttggat acccgcggct tcatcgaagt ggaaacgccg atgatgcagc
     5461 cactctatgg aggagcgata gcgcggccgt tcgtcacgca ccataacacg cttgatctcg
     5521 atctgtacct gcgtatcgcc cccgagcttt acctgaagcg gctgatcgtg ggcgggctcg
     5581 agcgggtcta cgaaatcaac cgcaatttca gaaacgaggg catctcgacg caacacaacc
     5641 ccgaattcac gatgctcgag ttctaccagg cttacacgga ctaccgcggg ctgatggatt
     5701 tgtcggagga actgctgcgc cagacagcgc tcgacgtgaa tggaaccgcg ctgatcgagt
     5761 atcagggaac ccaattggat ttcgatcaca tccggcgttt cagcatgcgt gaagcggtcc
     5821 tcgaattctg gagcggcgcg gacaagccct ccgccgccga cctcgcggat cccgattggt
     5881 tggggcgcca ttccacgaaa ctcacgtccg gcgaggcctt ggccgaaatc ttcgagcgcg
     5941 tgtgtgaacc gcaccttata caaccgacca tcatttacga ttaccccgtg gaaacctctc
     6001 ccctttcgaa gaacaagccc ggcaacccgg aatttgtgga gcgctttgaa atctacgccg
     6061 cggggatgga actcgggaat gcgtacaccg agctgaacga tccgcaggaa cagcggcgcc
     6121 ggtttgaaat gcagatcgcc atgcgtgaac gtggtgatga agaagcgcac cagatggatg
     6181 aggattacgt gcgggcactg agctatggca tgcctccgac gggaggcgaa gggatcggaa
     6241 tcgacaggct cacgatgatt ctcaccaatt cccgctcgat ccgcgatgtg attcttttcc
     6301 cactgctgcg ccccgaaggc cccatcgggt tgctcgaaca acttcgtgag ctcgacgcat
     6361 aacggatcat gggtaagttc aacggcttcg aggcgttcat cgcccggcgg tatctcaggg
     6421 ccaagcggaa acaggcagtt atttccgtaa tcaccgctat ctccatcgtc ggagttgccg
     6481 ccggagtcat ggcgctggtg attgcgctgg ccgtcaacaa cggctttcgc aacaccctcc
     6541 agcggaatct tctgggagcg accgcgcacg tcagcgtcat ggagaaggag ccgtcgtatg
     6601 gaattcagga ctggcgtgag atcacggagc gcctgcggaa attaccgcat gtagtggcgg
     6661 tcgcgcccgc gctctacggc aatgtgtttc ttgccgggcc ggttcgctcc gacggagctg
     6721 tgctgaaggg cgtcgatgtc gattccgagt tgggaatcgc ttctgttctg cgaaatctga
     6781 aagaaggctc catcgatccg ctacggcacg ccggcgatga gatgtacctc gttttgggtt
     6841 cagggctggc gcgcgagaca ggcatgctca ccggcggccg cgtgcaggtc atcagcccgc
     6901 agggcgaact cactcccttc ggtccccggc cgtcgtatta ccgcatgcgc gtcgcgggaa
     6961 ttttcgagtc caatttctac gagctggata tgcgatgggc gtacacgtct ctcgcttcgg
     7021 cgcagcatgt cttgtcgctt ccggacgtgg tgaacgcgat cgaactcaag ctcgacgata
     7081 tctaccgggc gccggaggtc gcgcgggtgg ccgaagaggc ggcgggtccg aaatacgcgg
     7141 cgaccaactg gcaggaacag aaccggcaac tgctgaacgc gctgaagatg gagcgggctg
     7201 tcaccatcat caccatcggc cttatcgaac tggtggccgc gctgaatatt ctgatcacgc
     7261 tcatcatgat ggtgatggag aagtacaagg atatcgccat cctcatgtcc atgggcgcac
     7321 gaaaggagca gatcagaaag atcttcatgt tccagggcgt catgatcggc gttgtgggaa
     7381 ccgtcatcgg actgattgca ggctatggcc tcagcttcct ggcgaaccac taccgcctgg
     7441 tcccgctcga agcatccgtc tattcgatga gctttgtccc cttcgagccg cgctgggtgg
     7501 acggattgtg gatcgcgggc ctggcgattt tcgtaagctt cattgcgacg atttatcccg
     7561 cccgcagcgc ttcgcgtatt gctccggccg aagcggtgcg ctatgagtga gcggccgcga
     7621 gcctatgaaa ttctggtagt ggccgtggcg gccattatct ttctgggaac cgcgttcagc
     7681 ccgccgcacc tgatggacga tgtggatgcc gtccaggcac agatctcgcg caacatgctg
     7741 gagtcgggcg attgggtgac ggcccggctc gacggcgtga agtatctgga aaagccgccg
     7801 ctcaaatact ggatgattgc cgtctcctac atgattttcg ggatacacga ctgggccgcg
     7861 cggctgccta ttgcgctctc ggcggtgacg ctgtgctggg tggtgttccg gttcggccgc
     7921 tgggcctttt ccccgcgagc gggcttttgc gccgcgctcg cgctcgcaac gtgtatcggc
     7981 ctgttcctct tcacgcgcgt gctgattgcc gatgtgattc tcacgcttgc cgtggctgtg
     8041 tcgctgtatg gcttcctgcg ttgcctggac gaacaggaac gaaacacgcg cttgtggtcc
     8101 gcggttttcg cgggcggcat cggatgcggc ctcctgttga agggcctgat tgccgccgtg
     8161 tttccgattg cggcggcggg gctgttcctg ctgttcacaa aagaactgtt cgcacggcgg
     8221 acatggcgcc gccttcaccc cttcaccggc ctgctcgtaa tctttgccat cgccgccccc
     8281 tggcatctat tggccatcat cagaaatcct ccatatttcg atttcacgat gcacagcggg
     8341 cccggccagt atcgcggatt cttctggttt tatttcctga atgagcacgt cttccggttc
     8401 ctgaatatgc ggtacccccg cgattacaac acggtgccgc tggtgtactt ctggcttctg
     8461 cacttggtat ggctgttccc ctggagcgcg ttcctgcccg gcaccctcaa gctgggctac
     8521 aagccggtgg accgcgccgg acgcacgcgc cttctggcgc tctgctggat cggatttgtg
     8581 ctggtgtttt tcagcttttc gacaacgcag gagtattatt cgatgccggc ctacccggcg
     8641 tttgcgctac tgctcggatg tgctgtggcg gcggatacgg cgtggttgcg ggcgggtctg
     8701 aaagtgctcg taatcgtttg ctcgctggcg gccgccgcct gtgccgccat tctggtccaa
     8761 gtctggaatc tgccgtcacc gggcgacatc tcgaacgcgc tcacgcagaa tcccgatgcc
     8821 tacactctct ccatgggcca catgggcgac ctgacgctcg cgtcgtttgc ctacctgcgc
     8881 ccgccgctgg cccttgccgc cgttgccttc ctcatcggag ccgccgctgc attcttttgg
     8941 aagagccatc gggcttacct cggagtggcg ttgatgatga ccttgtttac gcatgccgcg
     9001 cgagtggcca tgatccaatt cgatccgtat ctttcgtcgt ggcacctggc cgaggcgctg
     9061 cgcagttccc cgcccggcaa actgatcgcg gaggatcaat attacacgtt ctcctcggtt
     9121 ttcttctacg ccggccgcga tgcgctgctg ttaaacgggc gcgtgaacaa cctggaatac
     9181 ggctctaacg cgcccggtgc tcccgatgtc ttcctcggcg atgacgactt gaagcgccta
     9241 tggcatggtt ccggatcgtg gtacctggtt gcccggaaac aggctgttcc gcacctggag
     9301 agcgtggtcg gacccggcgc gctcaacgta gtaaaagaaa gcggaggcaa gctccttttt
     9361 tcgaatcgcg cggcggtaca ctgaaatcca ggccgaggct acccaataat ggacgaacgc
     9421 gccttctaca ccgaatcaca gactacgaaa ccggccagat tgagctgccc gtggtgcaaa
     9481 accgccgaga cgtaccagtt gcgttggctg ctacgcaaga agaaagaccg gctgccggga
     9541 ggcggcggcg aacgcgaccg ggcacgcttt caaaaagctc aaagctacat ggtgctgatg
     9601 gaagataagg tcatgtgcaa gaacatgcgg tgcagaaaac gtttcgacgt ctccggcatc
     9661 aagagcatgg cgtttctcac ggaatgattt cgaagcgtac gggcttgttc ctgttcctcg
     9721 cactggcggt tggcgcgggg gtccttctga tgttcctgct gtggtccgta tggccgttcc
     9781 gtggaggctt ccagcgcttc atcgacgctc cggtcatcgt gcgggaggtc cagcaactca
     9841 gtgaactggt cagcgtgaag tacagcgtgc agaaggtagt tgggctgaaa gaggataaga
     9901 tcccctttgg cacggagtcc atccttctag tggtggaggg gcgtgtgctc ggcggcatca
     9961 acctcggtgc gctgcgggaa caggatgtcg aggtgcgagg gcgcaaagtg tgggtgcggc
    10021 tgcctccgcc cgaaatactg cacgtctatc tgaacgaaga acagacgaag gtgtgggacc
    10081 ggcgtatcac ctggtggaca ccctgggtgc cgttcagccc cgatctcgag caaagagcgc
    10141 gcctggccgc tctggagtcg atcaaagagt ctgcactgga gatggggctg tacgcggaag
    10201 cgcgaaagag cgccgaaacc gcgattcggg gcctgctgcg agcaatgggc gccgaatcgg
    10261 tggaatttgc gccaagctca tagtttttac gaaacggctt ccagatccca tccgaaaccc
    10321 ggccttccgg cgggccgcgt gtagattcct tcgggaccgc gagtcagcag gttgtcctct
    10381 tcgtaccgct tgtagacctc cggcggacct cccggtgcgg gcatgaacat ctccgcccac
    10441 ggcgagttga cgctggccat gatgaagtgc acggaatcgc gcgagccgcc cccatgcgga
    10501 atcacgggga tatcgtaggc cgcagcgagc gcgccaatcc ggcgcagttc ggtgaggccg
    10561 ccgcaccagt gtatatccgg ctgccagatc gcagccgcct ggtgctctaa aagctgccgg
    10621 aatccatagc gggtgtattc gtgctcaccc gtcacgatcc tggtggattt gatctgggca
    10681 ttcaggcggc cgaacccttc atagtcgtgc ggctgaagac actcttccat ccagtacaca
    10741 cgatatggcg caaccaggtc cgccatctcg agcgtgtacc gctcatccca cgccatccag
    10801 cagtccagca tgatttcgcc atccgggccc agagcggtgc gagcgcgctt caccagttcc
    10861 acgttcgttc gctgcccttc gcggccatcg gcgggaccgt gcggaatcgc cagcttcagt
    10921 tttgtgtagc cgaattcgac gtgctgctcg atgtcgtttc cggtgcagta ggctggaatc
    10981 ctcgccttgg tttggcctcc caaaagctta tacaccggca ggcccactgc ttttccgacc
    11041 agatcccaga gggcgatatc cactccgctg atgccgttga tggcaattcc cgcgcggccg
    11101 taagacatcg tagaccgcca gaggatgtcc cagatccgct ccaggttgaa gggatcctca
    11161 cctatcagca gcttcgtgag atgctgctcg acaacatatc cgccgcccgg cccacccgta
    11221 ccatatccct tcacgccctt atccgttgcg atctccaccc agaatccgcc cagtttcccg
    11281 ggatccggca ggaatagggc ccggctcgct ttatattcag ggtagatgga catcgggttc
    11341 gcgacctcca ccccacccgt agaccacgaa cccggcgccg gagtgtagga tggaacgggc
    11401 ctgcgaggcc gcatacccac cagccgcacg gacgtgattt tcagcccgct cttctcgccc
    11461 gccagaggag cctgcgatag agcggctgat ggaataagcg ccgcgggagc gaaccggaac
    11521 agatctcttc gattcatgtc tggtatgtat agagctttcg cacgtagaat tcaagccgtc
    11581 gcctacaggc atggtccgcg ccggatggca ccgatttgat acaatcgact ccgattcctt
    11641 atgtcgaaac tcgtcgtcac cttcggtgaa atcatgttgc gcctggcacc cccgggcttc
    11701 gagcgatttc tgcaatctcc tcagtttgtc gcgacgtttg gcggcggaga ggcgaacgtg
    11761 gcggtcgcgc tcgcgaactt tggaaacccg gctcgatacg tcaccgtgct cccgagcaac
    11821 ccgatcgccg atgcgttcgt tggtgagctg aggcgttttg gagtggacgc ttccgccatc
    11881 gtacgcagca aaggccgcat gggcatctac ttcgtagagc ccggagcgaa ccagcggccg
    11941 tcgaaagtag tctacgatcg cgagaacagt gcgattgcgc tggccaaacc cggtgacgtg
    12001 gactggaaca agacattcga cggcgcatcg tggttccatg ttaccggtat cacccccgcc
    12061 atcagccagg gtgccgcgga cctcgcgctc gaaagcgtac gggtggcccg cgaaaagggc
    12121 ctcaccgtat cctgcgatct taactaccgc aagaatctgt ggaagtacgg caagaccgcc
    12181 aaggaagtga tgtcggaact gtttaaaagt gtggacgtcg gtgtcgccaa cgaagaggac
    12241 tgccagatgt cgctcggcat ccaggccgac gtcgacgtac actcgggcaa actcgacggg
    12301 gaacagtacc ggaagctcac ggacaaagtg ctagcgcaat atcctaacct gaagatgctc
    12361 ggtgtcacgc tgcgtgaatc tttcagtgcg tcgcataacg ggtggtccgc ctgcctgaac
    12421 gaccgcgagc aattcatagt cagccgccgg tacgatatca cgcacatcgt ggatcgcgtg
    12481 gggggcggcg attcgttcgc cggtggcctc atctacgggt tgttgaacct gagctcgcac
    12541 caggaagcgc tggagttcgc ggtggccgca tcgtgcctga agcattctat tccgggcgac
    12601 ttcaatcgct tcactgttga cgaggtgaag ggcctgctga agggcggcgg ctccggacgc
    12661 gtgcagcgtt gagtacaatt taagttccca ctatggctct accggaatcc gccgtcacct
    12721 ttctgtccag gcacatcgag cggattcagc ggctcgccag gaaaaagcga atcgtctttc
    12781 ccgagggcaa tgacccgcgt gtcatcgccg ctgcggtgcg gctggaacgc gacggcgtgg
    12841 tggagccgat actgattggt ccaaagcctg ccggcgtttc cggtgtcgcc tccatcgacc
    12901 cggccgattc tccgctggtt cgtaagtatg ccgcagtgct ctacgaaaag cggcgcgcca
    12961 ggggtgtgac ggagtccgag gcggcggagt ttgcccgcag gcccctctat tttgcggcct
    13021 tgatggtggc ggcgggcgat gccgatggcg gtgtcggcgg agcggcaacc agcaccgcgg
    13081 ataccgttcg ggcggccttg cactctatcg gcatgccgcc gcgattcaag accgtgtcga
    13141 gcatgtccat cctcgccagc cgcgaatccg gcttgggcca tcacggcctg ctcgctttcg
    13201 cggattgcgc cattgtcatc gagccctcgg cagttcagtt ggcggaaatt gctattgcgt
    13261 cggcggaaag cactcgtgtc cttatcggtg tggaaccggc tgtagcgctg ctttcgttct
    13321 ccaccaaagg cagtgcgaaa cacaagcagg tggacaagat tatcgaagcg cttcgcatcg
    13381 tgaaggcgcg cgccccggac ttgcacgtcg acggggaatt gcaggccgat gccgcgctgg
    13441 cggaagcggt gggtagagca aaagctcccg gctccaccgt ggcgggccac gcgaacacgc
    13501 tcgtattccc cgacctcaat tccgcgaaca tcggctgcaa gatcgccgaa agaatagggg
    13561 gagtcgcggt gatgggtcct ttcctgcaag ggctggagaa gccgttcaac gatttatcgc
    13621 gcggctgctc tgtcgacgac atctacaata cggcggtggt cacagccgtt cagacccaga
    13681 cgtactactg agggagccga ctacatggac cctttatatg ttcgcgtcca cccacgcgac
    13741 aatgtcgcca ttatcgtaaa ctccgaaggt cttccttccg gtaccgaatt cccggacggc
    13801 ctgcgtctga aggactccat cccgcaagca cacaaagtgg cgctccacga aattggaacg
    13861 gcggagccca ttgtgcgata cggccagaac ataggatttg cgaaccggcc gattccggcg
    13921 ggggcatggg tccgcgaaga aatgctggac ctcccggccg caccggcgct ggatgcactg
    13981 ccgctggcca cggcggttcc gcccccactg cctgaactcg ccggttacac cttcgagggg
    14041 taccgcaacg ccggcggtac cgccgggacc agaaacattc tgggcatcac cacgacggtg
    14101 caatgcgtgg ccccgacggt ggaacacgcc gtcaaaagaa tccgctcgga gattctaccc
    14161 cactttccag gcgtggacga tgtggccgcc atcacgcatt cctacggttg cggtgtagcg
    14221 attgacgcac cgggcgccgc cgttcccatc cggactctgc ggaacatcgt ccgcaatccc
    14281 aacttcggcg ccgcgccgct ggtggtcagc ctgggttgcg agaagttgca gccgtcgaga
    14341 ttgttgccgg accaacggct accctcggcg agcgtgagcg aattgccggt gctcgaagat
    14401 gagccctacg tcatccgctt gcaggattcg gaactgcacg gattcggcga tatggtggca
    14461 gccatcatgc gcttcgcccg gaagcgtctg gaagaactcg accggcggcg acgggtggaa
    14521 tgccccgctt ccgatctcgt ggtggggctt cagtgcggcg gaagcgatgc gttctccggc
    14581 gtcacctgca atcccgccct gggatttgcc gccgaccttc tggtccgggc aggcgcgacg
    14641 gtgatgtttt ccgaagtgac cgaagttcgc gacgcagttc acctgctgac accccgcgcc
    14701 gtcaacgagc aggttgcccg cgatctgatt cgtgagatgc gctggtacga cgaatatctg
    14761 gccggagggc tggccgaccg gagtgcaaat cccacgcctg gaaacaagcg gggcggcctt
    14821 tcgaatgtcg tcgagaaagc gcttggatcc attgccaagg ccggaagttc ggcgttgatg
    14881 gcggtcgccg ggcacggaga gcgtgtgaca gcgaaaggcc tggtattcgc cgccacaccg
    14941 gcgagcgatt ttatctgcgg cacacagcag ctcacgtcga tgaatatgca tgtctttacg
    15001 accggtgaag gctcgcccta cggcctggcc ctggtacctg tggtgaaggt gtcctcacga
    15061 accgcgctgg ctgagagatg gcacgatctc atcgacgttg acgccggccg catcgctacc
    15121 ggaaatgcct ccatcgaaag cgtagggtgg gaaatcttcc gcctgattct cgacgttgcc
    15181 agcggcagaa agcgcacctg ggccgaaaaa tgggggctgc acaacgcgct ttctcccttc
    15241 aacccgggac cggttactta gcgcgggtta gacggcaccc tgtcgcgttc cctgcgaacc
    15301 ggccgggaaa ttgtacacta acaaacatga aggggacttt aatctgggca attagtctcg
    15361 ctgtgcttgc cggaactttg cttgggcaag acccgcgggg aggtattacc ggccgtgttg
    15421 tggatagctc cggagccatc gttcccggag tccgcatccg cgcaaccaac aatgaaacgg
    15481 gcgtgtcggc acccgcgaca tccggcccgg caggcacgtt tacgattcct tacctgctac
    15541 ccggctttta ccgcgtgacc gcggaactgc cgggcttcaa gagctttgtc cgcgataagg
    15601 tagagatccg cgtatcggaa ctcgtggacc tcaccatcaa catggaagtc ggcgatgtaa
    15661 cggaaacggt ggaggtctcc gccgaatcac cattgctcga cacggctaat tcttcgctcg
    15721 gccaggtgat ggaccagcgc cgcatcgcgg aactgccgca gcgcggcgcc aacccgctgg
    15781 aactggcgct gctgacgccc ggcgtggtga acgccaccaa cctccgcttg cgcaaagcca
    15841 tggcgccgga ggcgacttcg caaatcgccg ccgacggcgc gggcacgtac aacaacgagt
    15901 tccagatcga cggcatcagc aacgtctccg ccgaccgggg cagcggctac ggccgcgtgg
    15961 ccttcagccc gccccagtcc gccgtgcgcg agttcaagat gcagacctcc gcctacgatg
    16021 cgagcgtcgg taacaccatg ggcgccctgc tgaatgtcag cacggccagc ggcaccaacg
    16081 agtaccatgg cgaggcgcac tacttcctgc gccacagcgc tctggacgca cccaattttt
    16141 tcgccaacaa gaacaaccag cggcaacccg tttaccagga ccaccgctac ggcggctcca
    16201 tcggcggccc gctgatcatc ccgaagctgt ataacggcag gaacaggacg ttctggttct
    16261 acacggcgga gcagaacccg ttcggggttc cggtgcagtt cacgcgcacg gtgcccacgg
    16321 tcgcgcagcg catgggcgac ttctccgccc tgctcgcggt gaatcccacg acgtaccaga
    16381 tctacgatcc gctcaccacc acgcccgcgg ccaacggccg tttccagcgc ctgccgttcg
    16441 cgggcaatat catcccgcaa aaccgcctgg accccacggg cctggccctg gtgaaccttt
    16501 atccgatgcc gaacgtgccc ggcaccaatg acgggcgcaa caacttcttc atcgcgggga
    16561 aagctttcca ggactactac gtgcacctgg tacgcctgga ccatgccttt tccgacacgc
    16621 atcgcgcgtt cctccgcgtg cactacgaca tgtggcggga acagaagaac aacgatttcg
    16681 gcacctggat caacggcatc catcagaacc gtcccaatcg cgggatcgcc gtggacgacg
    16741 tcatcgtgct gaaccagagc acggtgctga acctgcgcta cggcatgaca agcaccaagt
    16801 ggtggcagta ccgcgtgacc cgcggttacg accttgccag cctcggcttc tcgcccaacc
    16861 tgctgcggta cctcgatccg ggacagtcgc ccatcccgcg catttcggcg ggcgcatatt
    16921 cgcagatctc ctggtgggaa aacccggggg acggcgtgaa ctccagcctg acacacatgt
    16981 tcctggccaa tgtgaaccgg ctgcaagggc gccacaacat caagttcggc tcagatttcc
    17041 gggtcttccg tagcttcaac aaccggcggc cgattggcgt cgcaccggac ttcatattcg
    17101 gcacgacgta cacgcgaggt ccgctggata cgtccgccgc cgcgcccatc gggcaggacc
    17161 tggcgtcgat gttgctaggc attcccgacg gcgagatgca gatcgtggca agctcggcgc
    17221 tccaacacaa gtacctcggg ctgtacattc atgacgacgt gcggctgacc cggaagctta
    17281 ccgtaaatct cggattgagg tatgaactgg agcggccctt caccgaacgt tataaccggc
    17341 tggtggcgga tttcgactcc ggctccagca gccccatcga ggctgaggcg cgcgccaatt
    17401 acgcgctgaa cccgatcccc gaactgccgg tggacgcctt ccgtgtgcgc ggcggtctct
    17461 catgggtgga cgagaaccgg cgcagcccgt atcgcggcga gacaaacaac ttcatgccgc
    17521 gcatcggctt cgcctaccaa gctaccaaca gcaccgtgct gcgcggcggc tacggcctgt
    17581 tctacgactc catcgggatc atgaaagtgg tgccgatcca gaccgggttc acccagacca
    17641 cgccgattca ggcgtcgctc gataacggcc agacgtattt cgcgaccacg tccaatccgt
    17701 tcccgaacgg actgatcgtg ccacagggac cggagggcgg actccgcacc agcctcggcc
    17761 agaacctgga cttctacaat ccggatctca agcatggcta ctcgcagcgc tggtctttcg
    17821 gcgtacagca aaccctgccc gcgcagttcc taattgacgc ttcctacgtc gcaaatcgcg
    17881 ccacgcgcct cggcgtggac cgccagatca acgtgacccc ggcgcaatac ctgagcacca
    17941 gccccacgcg tgaccaggcg cacatcaact acctgagcca gcagttcccc aatccattcc
    18001 gaggcctgga cccgatctac ggcgccaaca tctcgcgcgc gaacctgctc cgcccgtatc
    18061 cgcagttcgg gaacatcact gtcgaagagc ccatcggtta ctcctggtac cattccctcc
    18121 aggtgcgcgg ggagcggcgc ttcgcccagg gattcacgtt ccagttggcg tacaccttct
    18181 ccaggctgat ggaggccact cagtttctga acaccgtgga tccgatgccc tatgagattg
    18241 tttcgagtct cgaccgccca catcgccttg ccgcgagcgg catctgggaa gtgcccgtag
    18301 gacgggggcg gcgcttcggc agcggcatgc accgcgcgct cgactacgcc atcggcggat
    18361 ggcagatcgg cggcatcctg accaagcagt ccggcccgcc gctcgatttc ggcaacatca
    18421 tcttcaacgg cgacccggac aacatcaaac tgcccagcgg ccagcgggac gtggaccgct
    18481 ggttcaacgt ggacgcgggc ttcaaccgca atgcgcagca gcagttggcc tccaacctgc
    18541 gcaccttccc gctgcgtttc agcggcttgc ggggcggcgg ccagaacagt tgggacttct
    18601 ctatcgtgaa gaacttcccc ctgcacgagc aggcccgggc ccagttccgc gccgacgtat
    18661 acaacgcctg gaaccagacc aatctgaaca cgccgaacat ggcgccgaca aacaccgcgt
    18721 tcggtcgcgt caccagcact acgggcgacg cccgcaactg gcagctttcc ttgaaggtga
    18781 tgttttaacc agggacccgg acttccgctt acactcgggc attacactga gattgtggaa
    18841 gaagtacgtg tcacaagttg ggcacacctc gacgaacagc tttatgcgga atcgtggttc
    18901 cagccgcacg ggcgttttcg ctctccttat gtttttcgcg gagtggcggc cgccggctcc
    18961 gatttgaaga cctgcctcat gcggttgggc gggcactacg gagagttgga ggacgacctg
    19021 ttgcgcaatt tccgcaagta cgcgcatcgc gaggcggcgc ccggcggctc ctggtggaac
    19081 tggctcgccg tagcgcaaca tcacggtctg cccacccggc tgctggattg gaccttttct
    19141 ccctttgtcg cgcttcactt tgccaccgcg gatttttcgc tgttcgcggc cgacgccgtc
    19201 gtctgggccg tcgactgcat cgcagtacag gagttcctgc ccgaaaagct gcgggagatt
    19261 ttgaaacagg aaggcgccac ggttttttca gccgaaatgc tggaccgcta tgccggaaac
    19321 ctcgcggatt tcgaccggcg cgtgcgggaa gaacagggcg agtgcgtggt cttttttgag
    19381 ccgccgtccc tcgacgaacg catcgtcaac caaatggctc tattttcgat gatgtccagc
    19441 gccgaagcgg atctgtggac ctggcttgcc cgacgggagc agaaatccaa acggaagtta
    19501 taccgcaaga tcgtcatacc cgcgccgctg aagtgggaaa tacgggataa gctcgatatg
    19561 gccaacgtga atgagcgggt tatgtttccc ggcctcgacg gacttagcgc ctggctgagg
    19621 aggtactaca gtccgaataa catcattgaa ataacgtatt ttgaaagtga cggccaacct
    19681 cacacttatc cggcggtcat caccagcatc agcaatgcca tgatggaagt cgcttaccat
    19741 tgcgagcgcg aaggccgccg cacggcacgg ttgtttacgc gggaaggcag catttggtgg
    19801 gacgaatgcc gggatagcgc ggtagcggtc aatcccgaac cagccgcgga gaagaccttt
    19861 cccggcaatc tcccgggagc gcaccaggct ggcccggccc aagtgtaagc ccgggccgat
    19921 tgtacaatag gggttccgtg gataaacgaa gaatgtgccc gaactgccgg gcgtttatta
    19981 ctacatccga caaggtctgt ccctactgcg atactccggt ggggccaaga gcagtggatc
    20041 gcaggaaatc cgccgaaata tccgccggat tcattcccca cgcccgattc gtcaccgttc
    20101 tcattcttac tatcaatttc gggctgtacg ccgccactgc ccttttcagc atgcaaaaca
    20161 gcggtgcgtc attcatggat atcgacggcc gcactctgct gctgttcggg gcgaaataca
    20221 acgagtttat cgccgccgga cagtggtgga ggctgatcac cgccggcttc ctgcacggcg
    20281 gtctctttca catcctcatg aactcctggg tgcttttcga tctcggcgcg caggtggagg
    20341 agctatacgg cgccaggcgc ctgatcgtca tctatttcct ggccacggtc ttcgggttca
    20401 tggtgagcac gtggtggtcg ccgtctcttt cgataggagc ttccgccggc atcttcggcc
    20461 tgctgggagc gatgatcgcc cttggaatgc gctccggcac ggccatgggt gcggcgatcc
    20521 ggggactcta cattcgttgg gcggtgtacg ggctgctcat cggtctcctc ccgtttttcc
    20581 gcatcgataa cgccgcccat atcggtgggc tcattgcagg attcgtcacg ggatttgtag
    20641 ccggaactcc cctgccgcgc cgctctgtac cggagaagtt gtggggcctc gccgcgctgg
    20701 cgtgcttatt gatgacagca ttcgcgtttc tccagatgtt cctgttcttc ggtgtggcat
    20761 ccaggtttta gttgtcgccc tgtttgaaca ggctcgcacg gtccagacgc tcaccccgga
    20821 aaaacacggc ggagatacgc tccgtagccg cgatttcttc gagtgggttg ccatccacaa
    20881 gaagcatact cgcctcacag cccggacgta tcagccccaa ccgatccgac gcgcgcaggg
    20941 cagccgccgc attacctgtt gcggccgtga gagcatctcg cgccggaacg cctgcttcca
    21001 cccacaattg cagttcgcgg tgcacggccg gaccgtgtgt aacgaggaga ttgccggcat
    21061 cggatccggc caccagcgga acccccgctt tgtaagctcg ctgcaggttg gtcattgcga
    21121 gcttcagatt gggttggagc agactgactt tctcacgcag cggggccgcc tgtccggaaa
    21181 caagaaagtc gtgcgtcgca tgcagcagcg gcgccgggcc aacctgctgt gcaagagagc
    21241 gttccagcaa ggcttcggag ccttgtgcaa tttgttgaag agcttccacc gcaaccagtg
    21301 ccggcacgaa catcactttg cgccgtgcca tggcgttcca tatttcatcc ggaatttcgt
    21361 cgcgcgttga accgtgcgcg atggcgtcgg cgcccgcttc caccgcttcc cgcacatccc
    21421 tgctgtcgcc ggtgtgcacc acggcaggca ggctgtgcgc atgggcttcc acgacgattg
    21481 ctttcagaat ggacgtgtcc aggcgaggga acactctccc cgaggcgcct gcttcaagaa
    21541 tggccttaat ggcctgaacc ccctgcccgg ccaggtcctt cacttcctgc cgggcctgtt
    21601 caaccgaggc cggcgtacgg accacggttc gtgtaagggc atcgcggaac ggctgcggca
    21661 cagtgtcgaa atactctgtt ccgtatccct ccggcgccgt gaaaaccggc cccgtcgtaa
    21721 agacttccgc tcccaaccgc tctccacttg cgatctgccc gcccgctcct ctgacaagtt
    21781 ccaaggggtc tcccacgctg cgcaccaccg tcacaccgct gtacaggtat gccgccagcg
    21841 cgcgacggat ggctttatgc ggttcgtatt cgccgggctc ctcatacacg cctcccggta
    21901 ctgcgaggtg aacgtgagtg tcgatcaatc ccggcagcag ggtctttccc gcgccttcga
    21961 tcgtttcggc tttcacgcca gcgccggacg gatcggaagg ataaagggta tcaatccggc
    22021 ccccccgaat gagaacggtt gccgccggaa tcacggttcc gtccccggcg aagacccgca
    22081 cgttgcggat gagccaggtg cggtcgcgat acatctgccg ttcgagaatc ctagccttca
    22141 cgatattgtc ccggctgtaa aactgccatg cgcccagcag caggaagggc gcgagaatcg
    22201 cggccagcca gagcttgctg gagcgtcgca ccttttcttc cttttcccag cggaaaagct
    22261 tcactccgat aaacgttgcg agcagcgtag tcaccagcag cgcaacaatc ggaacgatat
    22321 tcgcggccag gccttcccgg cggaccagga tgctctgcat tcccatgaac aaatacggcg
    22381 aagggaggaa ctgcgcgatg gcctgaagcc aatgcggcat cgacgtcagc ggaaaaaggc
    22441 tgaggatcag catcggcatg tagaaaagct ggatgatgat ctggctctcc tggagagagt
    22501 tcacaaccga tgcgataatc agtcccatgg ctcgaaaggc gaagactcca atcgagagga
    22561 acaccagcag ttcgggccag cgctccggcc aaggcatgcc gtagatcagg tgtgcaagca
    22621 ccagaatcat caagccggaa gggaagtagt ttacccaacc cgtaatcagc gaggacacca
    22681 gcaccggagt ggccgtgatc ggcgttactt tgtaccggcg caggatgtta aactcacggt
    22741 cctgcaccgc acgaactcca gccccgaaga aaccattgcc gagcacaccg ataatcagca
    22801 ccatgctcac cacctgatag atggcggcgc cctgcgcggc ttcagacaac tgcccgaaca
    22861 tgaagaagaa cagcagcggg aatacgtagt tgaagaaaat caccacgcga tcgcggaacg
    22921 tcagcttcag ggtgatcttc gtgtatgcca ggtaagtctt caatctctga ggctctttcc
    22981 ggtcagctcg atgaacacgt cttcgagcgt cggcctcttg agatgaatgt cggcgagttc
    23041 gatcccctgc tgatccaccc actttacaag ctccaccagg gtgcgcgtcg gatgcgtcga
    23101 cgtggccgtg agcatcatac gatctttgct gagcgatatc ctttgcgcac cggcgacggg
    23161 cggcatcacg ccttcggcca caggggtttc acagcgcatc tcgatcaggg aatcgcccac
    23221 ggttttcgac tgaatctcgc ggggtgttcc aatcgctata atctttccac catccacgat
    23281 cgccacacgg tcgcacagct tctcggcttc ctcgatgtag tgcgtagtaa ggagaaccgt
    23341 acatccggca tgcttgagtt gctggattaa gctatgaatc tccagccgga cctgcggatc
    23401 gaggcccgtg gtgggttcgt ccagaaacag caactgcgga tcgttcacca tcgatagagc
    23461 gagggcgaga cgctgcttct gtccaccgga gagatgcacg taatactcgt cgcgcttctg
    23521 ccaaagctgc agccgctcca gaagccgggt ggcatcggcc gcgcgcgaat aaaaggccga
    23581 aaacaggtct acagcttcgc gcacggtgat cttttccgga aggttggttg cttgcaggca
    23641 gactccgata cgatccttca gcgcccggac ctgtacggcg gggtcgcacc ccagaaccga
    23701 cacatcgccg gcggttctcg tgcggaggcc ctccagaatt tcgaccgtgg tcgtcttgcc
    23761 ggcgccattc ggccccagaa gaccgaatac ttcgccctcg gctacttcaa aatcgatacc
    23821 gcgaatggcc tcgaaagccc cgtagctctt gcgcagtccg cacaccttga tgggagctac
    23881 ctgtgattgg gccattcgac acgaaggagt cagaccgcga caggagcttt ggacagtacc
    23941 tcgattgcgg accggcagaa ggcgggcagg tcctccggtt tgcgggacga taccaggttg
    24001 ttgtccacca cgacttcgga atcctcgtac agaccgccgg catggatcac gtcgtctttg
    24061 atcgcgaaga agcacgtcac gcggcgccct ttgagaatgc gcgccgagca cggcacccac
    24121 agcccgtgac aaatggcggc aaccagtttg ccctgatcgt ccatctcttt cacgaactgg
    24181 ttcgcccgtt catgacgccg tatatggtcg ggagcgaagc cgcccgggat caccacaccg
    24241 tcgtaatcct gcgctttcac ctcgtcatac gatacatcgc aaacaatcgg atatcccagc
    24301 ttgctggtgt aggttgtacc ggcgcgcgcg cccaccgcca cgacctccgc gcccgcttcc
    24361 ttaaagcgaa aataagggta ccaaacctcc atctcctggt agaggttgtc gaccagaaca
    24421 gcgatcctct ttccctttag ttccatagtc tatttattgt agcccggaca gaaaaaaagc
    24481 cggggttggt tgcaccccgg caaagccgca tcgatagact accccatgac ctgtcccggt
    24541 gaacctactt catgtacttt tcccgctcga tctcgtattg ggattgccgg atcacctcgc
    24601 gcggcaaagc atcaacattt tcagcacggc ctttgaggac atgcacctgg tgatgaccgt
    24661 ccagtatcgt gccgtccggc ctcgccttca ggcaatgctc ctgccccggc gctagcgatg
    24721 ctttcaactt ctcggttgtg aacttttcaa acacggccag cttcgcggga ctgagcgaac
    24781 tagccgggtg cagtggcttt aacggtggct gctcgctcat cggggagcaa agctctccgc
    24841 gtaatgatcg atccatctcc gctgctcggg cgtttcagga atggatgttc cccgcgccgc
    24901 gcttaaccgg gtgatcgcgc tttccggatc gatacctttg gtgaggagta aacacgcggc
    24961 tacaagcccg gtacgcccta tcccctgcct gcaatgcagc acgacattcc ggcctgccgc
    25021 gagctcagct tccagctttg caagagcctt tccgagatta gcttccgagt ccggcacctg
    25081 gcggtcgggg atcgggtaag agagaaactt catgccgtgc gcctcgactt cagccttttc
    25141 ctttagaatg ccgaggtcgc gttcctcttc atcggtcagc agcgaaaaca cggtattcac
    25201 gccttcgcgc tgccagcttg tgatttcatc ttccagccat tcgccgccgc gcggacgcgc
    25261 ggccaaggcc agctttcccg gccatggtcc gtcaacccag tgcagctttg tccacatcat
    25321 cggcgctgat cttcatttgc ggtaatcagg gagccggaac aaaacaccgg tgccggctcc
    25381 ttgattcgta cgcagcttca ggctattcgg ccagcgatcc ctcaccgctg tcttcggaaa
    25441 ggccaccggt gaacgtgagg cgggtctcct cctcgtatcc cggaattcct tccatcacgg
    25501 tttcgggttc ggtgatcggc tcttcttcga tgtcctcacc cgcgatcttc accgtccggt
    25561 aatactccat gccggtaccg gcgggaatca accggcccat gatcacgttc tccttcaaac
    25621 cgcggaggtt gtcgaccttc ccattaatcg ccgcttcggt cagaacacgc gtagtctcct
    25681 ggaaggaggc cgcggagatg aacgagtccg tggagagcga cgctttcgta ataccgagca
    25741 gcaccggccg gccctgtgcc ggacgcccgc ccgcatccat tacgcgctgg ttttcctcgc
    25801 ggaacttgaa ccggtccact acctcttccg gcaggaactc cgtgtctccg atatcgtcga
    25861 tcttcaccca gcgcatcatc tggcggacga tcacttccag gtgcttgtcg ttgatgttga
    25921 caccctgaag ccggtagact tcctggatct cgttgaccag gtatttctgc agctcctttt
    25981 cgccgagcac gtgcagaatg tcgtgcgggt tgcgggggcc atccatcagc ggatcgccgg
    26041 cccgtacccg ttcgctctcc tgaacgttga tgtgaacgcc gcggggcaag gagtattccc
    26101 gctcctggcc atcgtcaccc atgatgtaga tcttccgggt gcctttcgaa acatcgccgt
    26161 acttcaccgt tccattgatc tcggcgatga cggccgtctc gcgcggtttg cgcgcttcga
    26221 acaactcgac gacgcgcggc agaccgcccg tgatgtcttt ggtctttgtg gtggcacgcg
    26281 ggattttcgc gacgacgtcc cccgcgaaga tctcgtctcc ctcgcgcacc atcagatggg
    26341 catgagtcgg catgaggtat cgcttctcat cggctcgcga cgtaccgctc cggacaatga
    26401 cggatggcag ccgcttctcg tcgggcgaat cgctcacaac ccactgcgac atacccgtga
    26461 tttcgtccac ctgctcgcgt accgtcaggc cttcctgcaa atctttgaag tgaacgacgc
    26521 cgctcacctc cgtcaggatc gagaaagtat agggatccca ctcgagcaga atctgattcg
    26581 ctttcacggg atcgccgtcg cccaccagaa ccttggcgcc gtagacgacc gggtagcgtt
    26641 ccttctcgcg ctgcttgtcg tcgacgatgg ccaaaattcc ggtccggttc atggcgatca
    26701 actcgccgcg ggcgtttcga acggtggaaa tcccgatgta cttcacgaaa ccgtcggact
    26761 tcgcgtcctg cgtcgactgt tcgctgaccc tggtagccgc accaccgatg tggaacgtcc
    26821 gcatcgtcaa ctgcgttccc ggttcgccga tggattgcgc cgcgatcaca ccgacggctt
    26881 ccccacgctc gaccatgcgg ccggtcgcga gattccgtcc ataacaaagc tggcacacac
    26941 cacgtttcga ttcgcaggtg agaaccgagc ggatcttcac ccgctctata ccggccgcct
    27001 gaatcgcgcc cgcaagctct tccgtaatct cgtggtttac gttgacaatc acgttgcctt
    27061 cataatccaa ctgatcttcg agcgctacgc gtccgacgat gcggtcccgt aacggttcaa
    27121 tgatttcgcc ggattcgatg atcggctcca cgtaaatgcc gtcgccggtg ccgcaatcga
    27181 gctcggtgac aatgacgtcc tgcgccacgt ccacaagccg cctggtcagg tagccggaat
    27241 cggccgtctt cagagcggta tccgccagac ctttccgcgc gccgtgcgtc gagataaagt
    27301 actgcaacac gttcagtcct tcgcggaaat tggcggtaat gggcgtttca atgatctcgc
    27361 cggagggcct ggccatcagg ccgcgcatgc cggaaagctg ccggatctgc tgtttcgaac
    27421 cacgagcacc cgagtccgcc atgatataaa tcggattcag gtaacggccg gtccggtcgt
    27481 cttcctccat cacccggaac atctcttcgg agacgcgctc ggtgaccttg gaccagattt
    27541 caatgatctt gttgtagcgc tcgccctggg taatggcgcc ttcctggtac tgctgctgga
    27601 cttcgatcac ctgcttctgg gcgtccttta ccagcgccgc cttcgagccg ggcaccacca
    27661 tgtcgtcgac gccgatggag ataccggcgc gcgtagcata caggaagccg agactcttga
    27721 tttcgtcgag catccggacc gtggtttgaa gaccaaacct cagatagcag taatgcacga
    27781 gttgggaaag acccttcttc ttcagcaggc cgttgatgaa gggcatgtct tccggcagaa
    27841 cgtcgtttaa aatgacgcgg ccgacggtcg tgtccatgta ctgcttgttg aactcgatcg
    27901 gctccgtgtg catgacgttc tgattgtcga acgccttcga gaggtcgatg acgcggcccg
    27961 tatacctcag cttgataggc gtgagtgttt cgacctcgcc catttccagc gcgatcagga
    28021 cgtcttccat cga
//
LOCUS       DASTMK010000119        11259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_133940, whole genome shotgun sequence.
ACCESSION   DASTMK010000119 DASTMK010000000
VERSION     DASTMK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133940"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFQ79_04960"
     CDS             complement(<1..681)
                     /locus_tag="VFQ79_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139033.1"
                     /translation="MKEKHEAGASRRQFLKAATSVVPALPIGASAYAQGSDIIRVGVI
                     GCGGRGTEAATQAMHVDKGVRIAAMADLLMERAQEKRNLIRVKYPDQVTADDAHCFSG
                     FDGYKHVIESVDVVIIANASKFHPLHMTAAIEAGKHVFVEKPHAIDPAGCKAVRAACD
                     LAKKKNLCVVSGLQSRYHPGYRETIQRIHDGAIGEIIAVEENFLRAPYVLYPRKPDLS
                     EVEWQGSNQ"
     gene            complement(698..2158)
                     /locus_tag="VFQ79_04965"
     CDS             complement(698..2158)
                     /locus_tag="VFQ79_04965"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU0139034.1"
                     /translation="MRKALLTFGLLTSLCSAAAAEPVRALIITGETDLPYHDWRISTP
                     FLREVLERTGRFVVKVIEEPRAIDSDALSGYDVLVLNYTGPRWGERAEAAVEEFIRSG
                     KGMIAIHGVSYGPFYGQNLKTRQMAGAPWPAYGDLMGASWKLENIGHSRRHVFPVKWV
                     DSNHPVARGLASTFLANDELYHKMDLKPGVQVLASAFSDTKMSGTGKEEPIIWTVAFG
                     KGRVVHMTLGHDLSAMTQPGFVTAFARGAEWAATREVTLPASISAHPEPAKDAVRVLA
                     VTGGHSYPTSFYSVFEGYSGIAWSHATSQSQAFRENMKSRFDVLVLHDMHETIADKER
                     ANLQAFVEAGGGIVSIHHAIVDYTSWPWWYEEVIGGKYFTKAVAGHAASSYREGVEVI
                     ANPVRAMSSHPVLRGVGPIVVTDEVYKGMWHSPRITVLMETEHPENDRPVVYLGPNAK
                     VKSIYIQLGHESETFHHPGYRQLVRNGILWAAGRLE"
     gene            complement(2151..3020)
                     /locus_tag="VFQ79_04970"
     CDS             complement(2151..3020)
                     /locus_tag="VFQ79_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0139035.1"
                     /translation="MFQIGFHTDAFNSSYWNFEQCLAWAQRNQVHFIECGTIDGVSWI
                     HGLGYQPHIALWEDPLLLRRKMDRFGVEFSQLDAAFPLSLPEGATLGVEYVLHTIRWA
                     KLAGCPRVDTTDDRNRPSGMTDREALDHMRRIYSEIVKIAEAYQVVINIEPHGYYTTR
                     PEFMAEMLGFVESPYLGMNMDTGNTFIAGADPVVFIDRFHGKITHVHIKDVSATLAAA
                     ARGELTGIAVSHCAIGEGVNADNIRRCLELLRRCGYDGVLSIECEGQGGPMIERSLEW
                     VRRVVAEVEQAHA"
     gene            3170..3454
                     /locus_tag="VFQ79_04975"
     CDS             3170..3454
                     /locus_tag="VFQ79_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0139036.1"
                     /translation="MIFSYRDKRTQDFANGRRVKAFSGFERSAQIRLDRLEAATSMKD
                     LAALPGNFLEALKGDRRGQYSIRINEQWRICFEWPNGSPGPRNVEIVDYH"
     gene            3471..3773
                     /locus_tag="VFQ79_04980"
     CDS             3471..3773
                     /locus_tag="VFQ79_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEU0139037.1"
                     /translation="MARTPIHPGEHLREELETLQMSAAELSRQLGVPTNRVTGILNGQ
                     RSITGDTALRLAHFFGTTPEFWLNLQSLYDLRLAQQKSGKAIERLPTLKQRTLIPA"
     gene            complement(3895..7902)
                     /locus_tag="VFQ79_04985"
     CDS             complement(3895..7902)
                     /locus_tag="VFQ79_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU0139038.1"
                     /translation="MRRPAKILFWAGGAIIVLVVGISIAAFFVLQSEWFYNKVRDRIV
                     YEVERASGGSSQIGSFQIDWKTLTARVRGFVLHGTEPPDAPPLFRAGQIEVGLKIVSL
                     LRKDVDIASLIVDQPRIHILVDENGKTNIPEPKVKRTSTKSPIETILDLAVRDFRLND
                     GEITYDSRTVPISARGEDLNAHFTYNPAVPRYEGNVEFSRLNVDTPRFSPVAFALDAT
                     LAMERDRLEISKARLGMGQTSITLSGTVQDFRVPQAGLNFVADLSVPELAGQFKLPVE
                     RRGTVAIRDGHASWSSEAGYLVTGKLTGRDLGFRRAGVNIPRAAFQADLRVSPQGLRL
                     DGLHTAALGGVFNGSLGLEEFERLRVAGEINGFLLSELTQVVNRPSLAWSGVVSGPVR
                     VSGTLTGGGLRGVEVTGGFKVSPAEGPNPVHGSVEVAYNQRTGTLRLGDSSIEAGDTR
                     IDFSGTLGENLRVHLLSRDLTDLLPAIHMVSEKAPEQLPVELAAGGTAEFSGNVLGKL
                     NAPQITGRLQLTGFVVQNQRFDRLAATFKIENDLAGVDKLSVRRGEMVAVAQGEAGLE
                     NWRLAPDSTIRASASLEKAQLGTLLAEIGRKEPVDGTLRGSVDVSGTYGSPDATIKAT
                     IQNFRAWEETFDSVRADLRVTGGRVELIAGQLQLGAARLDLVGAYDHPEDDWKNGKMH
                     FQTSTKGFTLGQFKTVQRLGPQVQAAIGARAEGSADIVNGAFRLGSLNGEVSIRDLMF
                     EKERVGNIRLLATSKDDRLTVQTNAVLRRSKVEGNAEFRLAGDYPGKGHIRFTPLSFS
                     TIQQLRPGQKGPLPFEGLIAGQATFEGPVLKPDNLAGQVELTELRVWPNAGGALRTTA
                     DPKELALQNDGPIVLNFRGKTLDVQRAVFTGKGTNLSASGSFSYQDKNPWNLGLKGSV
                     DLSILEVFDREIRSSGTMSVDASLKGSIADPQVFGQVRFTAASFYHEALPNGIDGLNG
                     SIDFDRGRATIEEMKGTTGGGAVAVTGFLGFGGEELVYRLGVNLKEVRVRYPEAVSTT
                     LNAALNWTGTARQSLLSGTATIIRTGFNPQTDIAGLLASSARPVPTPTSPNEFLQGMQ
                     LDIHVENAPNLMLQTSYTQDLQADIDLHVRGTGTRPALLGRVTVSEGEINFFGSKYQI
                     NRGEVNFFNPVKIEPVLDIDLQTEARGVTVNITLSGTPTRMNVTYRSDPPLAPSDIVA
                     LLAMGREPDAAQNLASSQVVTNQSFLATGANTLISQAIATPVSSRLQRFFGVSRLKID
                     PMLTGLEGTPQARLTIEQQISPNITLTYVTNLTETQQQIVRLEWDFSRQWSVVAVRDA
                     NGIFGIDFNYKKRFK"
     gene            complement(7899..8549)
                     /locus_tag="VFQ79_04990"
     CDS             complement(7899..8549)
                     /locus_tag="VFQ79_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139039.1"
                     /translation="MKHAVCALLLLPVASYGAGGELIDRIAVRVGATVITQSEIVRDL
                     RITAFLDGRPAELNPDTMRKAAERLVERALIRREMDANRYPPAQFVEANHLLDQIKGR
                     FANEDAFRSSLAEYEIREDDVRDHLLLQLTTLRFIEYRFRPAIQVPARDIETYYETVF
                     LPQAGQNHAERIPPLDEVRGDIEKILTNQRVDQSLERWLQETRLQVDIEFRDEVFS"
     gene            complement(8549..>11259)
                     /locus_tag="VFQ79_04995"
     CDS             complement(8549..>11259)
                     /locus_tag="VFQ79_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEU0139040.1"
                     /translation="EAELREGGVVLRFLTKPAWFIGRVTVQGVPDPPNRGQLENATKL
                     ELGSQFTERAFRQAIEKLEQLLKSNGFYRPRIESDFEAQPEVNQVNIRFFIEPGERAK
                     FAHPTLTGDLERPPEDIVGATSWKRLWGLLGWKSVTENRLQRGLENIRTAYRKDDRLL
                     ASVTLDDLKFRPDPNTVTPVLKIDAGPTIEIRTEGAKISGGKLRQLVPVYQEQSVDKD
                     LLVEGAGNLQSYLQSEGYFDARVDFKTAGPNTDKEVITFVLDRGPRHSLKHLEIAGNS
                     YFDTGTIRERMYLRTASLLQFRHGRYSEEYLERDRQAITDLYRGNGFREVKVSSRIEA
                     NHGGDPNDIAVYLQIEEGPQWFVSALEISGVNLRDYEYIQSLLHSSEGQPYSEINIAA
                     DRDAILSFYYNNGFPDASFDFTATPGPGPHQMQLKFIINEGPRYFIRQVLVGGLETTD
                     PELVRERILLRPGDALSQIAMNESQRNLYELGIFAKVDTAVQNSEGKERNKNVLYEVE
                     EARKYSLNAGVGAEIARIGGGAATNLTSPGGSAGFSPRISIGISRSNVLGVAHTVSLN
                     TRFSSFQRRGVINYLAPQFKGNQDINLNFTGIYDDSRDVRTFAAKRMEASAQVGHRLS
                     KANTAQYRIQIRRVSVDENTLKITPGLIPLFSQPVRVTVVGGSFIQDRRDDPLDARRG
                     IYNTVDLGVTAKFLGSQTDFSRLLARNATYHPLTRQVVFARQTTFGWMYSFASEDDPL
                     QDIPLPERFFAGGASSHRGFSENQAGGRDLTTGFPIGGKALLMNTLELRYPLVGENLT
                     GVLFHEAGNVYSSLRRLSLRQTQKDDSNFDYMVHAVGFGIRYRTPVGPVRVDLAYVPN
                     SPRFWGFEGTREELLFNCAAGPGPQCPRTLQRLSRFQFHFSLGQAF"
BASE COUNT         2284 a         3409 c         3203 g         2363 t
ORIGIN      
        1 ttgattgctg ccctgccact ctacttccga aagatccggc tttcgcgggt aaaggacata
       61 gggcgcccgc aggaagttct cttccacggc aataatttcg ccgatggctc cgtcgtgaat
      121 gcgctgaatg gtttcccgat agccgggatg ataacggctc tgcaaaccgg agaccacaca
      181 gagatttttc tttttggcca gatcgcaggc tgcgcgcact gccttacatc ccgccgggtc
      241 gatcgcatgg ggcttttcca caaacacgtg cttaccggct tcaatcgccg ccgtcatgtg
      301 aagggggtgg aacttggagg cgttggcgat gatcaccaca tcaacgcttt caatcacgtg
      361 cttataaccg tcgaagcccg agaaacagtg cgcgtcgtcc gcggtcacct ggtccggata
      421 cttcacccgg atcagattgc gtttctcctg cgcccgctcc atcagcaggt ccgccatggc
      481 ggcgatccgg acacccttgt cgacgtgcat tgcctgcgtt gccgcttccg tgccgcgccc
      541 tccgcatccg atgacgccta cccggataat gtcactcccc tgggcgtacg cgctggcgcc
      601 gatcggcagc gcgggaacca cgctggtggc ggccttcaaa aactgccggc gcgatgcccc
      661 ggcttcgtgt ttctccttca tcgattcctc caagccttca ttccaggcgc cccgctgccc
      721 agagaattcc gttgcgcacc agttggcggt atcccggatg gtgaaaggtc tcgctttcat
      781 ggccgagctg aatatagatc gacttcactt tcgcattcgg accgagatag acaacaggac
      841 gatcgttttc cggatgctcc gtttccatca gcacggtaat tcgcggcgaa tgccacatgc
      901 ctttatacac ctcatcggtc acgacaattg gaccgactcc gcgcagaacc ggatgggagc
      961 tcatcgcccg caccgggttc gcaatcacct ctaccccttc ccggtacgag gacgctgcgt
     1021 gacccgcgac ggccttcgtg aagtacttgc cgccgatcac ctcttcatac caccacggcc
     1081 acgatgtgta gtcgacgatg gcgtgatgaa tggagacgat gccacctcct gcttcgacga
     1141 aggcctggag gttcgcccgt tccttgtcgg ctattgtctc gtgcatgtcg tgcagcacca
     1201 gcacgtcgaa gcggctcttc atattctcgc ggaaagcctg gctttggctg gtcgcgtgtg
     1261 accacgcgat accggaataa ccttcaaaaa cgctgtaaaa cgaggttgga tacgaatggc
     1321 cgccggtgac ggccagcacc cgaaccgcat cctttgccgg ctctggatgg gcgctgatag
     1381 acgccggaag cgttacttcg cgggtagcag cccactcggc gccacgggcg aacgccgtga
     1441 caaagcccgg ctgcgtcatc gccgaaaggt cgtggcccag cgtcatgtgc accacgcggc
     1501 ctttgccgaa cgccaccgtc cagatgatgg gctcttcctt tccggtgccg ctcatcttcg
     1561 tgtcgctaaa ggccgaagca agcacttgga caccgggctt cagatccatt ttgtgataca
     1621 gctcgtcgtt ggccaggaac gtggatgcaa ggccgcgggc gacggggtgg ttggagtcca
     1681 cccactttac cgggaacaca tgccggcggg agtgtccgat gttctccagt ttccagctcg
     1741 ctcccataag atccccgtaa gccggccagg gcgcgcccgc catttgccgc gtcttcaggt
     1801 tctggccgta gaagggaccg taactgacgc catgaatcgc gatcatgcct ttgccagagc
     1861 gaatgaactc ttccaccgcc gcttcggccc tttctcccca gcgcgggccg gtgtagttga
     1921 gaaccagaac gtcgtaaccg gatagcgcgt cggagtcgat ggcgcgcggc tcttcgatca
     1981 ccttgacaac aaagcgtccc gttcgttcca aaacttctcg aagaaacggc gtgctgatcc
     2041 gccaatcgtg atagggaagg tccgtttcgc cggtgataat cagcgcgcgc accggctccg
     2101 ctgctgcggc agagcacaac gaagtgagca gcccaaaggt gagtagagct ttacgcatgc
     2161 gcttgttcca cttccgccac cactcgccgg acccattcga ggctacgttc gatcatggga
     2221 cctccttgtc cttcgcactc gatgctcaga accccgtcat agccgcagcg gcgcagcagc
     2281 tcgaggcagc gccggatgtt gtccgcgttt accccttctc cgatagcgca gtggctgaca
     2341 gcgataccgg tgagttctcc gcgcgcggcc gccgcgagtg tcgcgctgac gtctttgatg
     2401 tgcacgtggg taatcttccc gtgaaagcgg tcgatgaaaa ccaccgggtc cgcacccgct
     2461 atgaaggtgt ttccggtatc catattcatt ccgagatacg gtgattcgac aaagccaagc
     2521 atctcggcca taaactccgg cctggtcgtg taatatccgt gcggctcaat gtttataacc
     2581 acctggtatg cttccgcgat cttcacaatc tccgaataga tgcgccgcat gtggtcaagc
     2641 gcctcgcgat cggtcatgcc ggaaggcctg ttccggtcgt ccgtggtatc cacgcgcggg
     2701 cagccggcca gcttagccca tcggatggtg tgcagaacgt actctactcc aagcgttgcg
     2761 ccctcgggaa gcgacagcgg aaaagcggca tcgagttgcg agaactcgac gccgaagcgg
     2821 tccatcttgc gccgcagtag cagcgggtct tcccaaagcg cgatgtgcgg ttggtacccc
     2881 aggccatgga tccaactcac cccgtcgatg gtcccgcact cgatgaaatg aacctggttc
     2941 cgctgtgccc aggcgaggca ctgttcgaag ttccaataac tcgaattgaa ggcgtccgta
     3001 tgaaacccga tctgaaacat gtcttccgtt ggacgccgga accaaacctc aaggtccgat
     3061 gttccggtgt cgaggcatca ttatatccga gacgggttgg tgaagtgcgg agcggggcaa
     3121 catgtagaat tacgcttgac gtacagcata acacgcgcta gtctatttag tgatcttcag
     3181 ttaccgggat aagcggacac aggatttcgc gaacggcaga agggtaaagg cattttccgg
     3241 gttcgagcgc agcgcgcaga tcaggctgga ccggcttgaa gcggcgacgt cgatgaagga
     3301 tttggcagct ttgccaggca acttcctcga ggcgctgaaa ggcgacaggc gcgggcagta
     3361 tagcatccgc atcaacgaac agtggaggat ctgtttcgag tggccgaacg gttcgcccgg
     3421 tccacggaat gtcgaaatcg tggattatca ctgagggaag gagagaagac atggcacgaa
     3481 ccccgattca ccctggcgag cacctgcggg aggaactgga aacgctgcag atgagcgccg
     3541 ccgaattgtc gcggcaactt ggcgtaccaa caaaccgcgt gacgggcatc ctgaacggcc
     3601 agcgatcgat caccggcgac actgcgttgc gcctcgcgca tttcttcggc acgaccccgg
     3661 agttctggct gaacctgcaa agcctctatg atctccgctt ggcgcagcaa aaatctggca
     3721 aggcgatcga acgcctgccc actctgaaac agcggacgct gattcctgcc tgatcgtggg
     3781 aacaaacaca cgctgtggct cccaaacagg ccgccgctcg cgatctactc cagccggacg
     3841 atcagcggaa agtattgaac ttgatccgcc tatcgtctgc ggtaccgggt gtgcttactt
     3901 gaaccgcttc ttgtaattga agtcgatgcc gaaaattccg tttgcgtcgc ggaccgcgac
     3961 aacagaccat tgccggctga aatcccactc cagccttacg atctgctgct gcgtctcggt
     4021 cagattcgtc acataggtta gcgtaatgtt cggagagatt tgttgctcaa tggtaagccg
     4081 cgcttgcggc gtaccttcca gtcccgtgag catcggatca atcttcagcc tgctgacacc
     4141 gaagaaacgc tgaagacggc ttgagacagg cgtggcaatc gcctggctga tcagtgtgtt
     4201 cgctccggtc gcaagaaagc tctggttggt cacgacctgg ctggaagcaa gattctgcgc
     4261 cgcatccggc tctcgtccca tcgcgagcag cgccacgata tcggagggcg caagcggcgg
     4321 gtcggaacgg taagtgacat tcatccgcgt aggagtgccg gagagagtga tattcactgt
     4381 cacaccgcgg gcttccgtct gcaagtcgat gtcgagcacg ggttcgatct ttaccgggtt
     4441 gaagaaattt acttcgcccc ggttgatctg atatttgctc ccgaagaaat tgatctcccc
     4501 ttcgctcacc gtgacacgtc ccagcagcgc gggccgggta cctgtgccgc gaacgtgcag
     4561 gtcgatatcc gcctgaagat cctgcgtgta agacgtctga agcatgaggt tcggcgcgtt
     4621 ctcgacgtgt atatcgagtt gcatgccttg caggaattcg ttggggcttg ttggagtcgg
     4681 taccggccgt gcggaggaag ccagcagccc ggcgatatcg gtttgcggat taaagcctgt
     4741 gcgtatgatg gtggcggtgc ccgacagcag gctctgcctg gccgtccccg tccaattcag
     4801 cgccgcattc aaagtcgtgc tgaccgcttc gggatatcgt acgcgaactt ccttgaggtt
     4861 gacacccagg cggtaaacaa gctcttcccc accaaaaccg agaaatccgg tgaccgcaac
     4921 ggcaccgccg ccggttgttc ccttcatttc ttcaatcgtc gcgcgcccgc ggtcgaagtc
     4981 gatcgatcca ttgaggccgt cgatgccgtt cggaagtgct tcatggtaga acgacgcagc
     5041 cgtaaaccgt acctgcccga aaacctgagg atcggctatc gaccccttaa gcgatgcatc
     5101 caccgacatc gttccggaag atcgtatctc gcgatcgaaa acttccagaa tcgaaagatc
     5161 gacagaacct ttcaacccaa gattccacgg atttttatcc tgataggaaa agctcccact
     5221 cgccgagagg ttggtgcctt tgccggtaaa aaccgcgcgc tggacgtcca gggttttgcc
     5281 gcggaagttg agcacgattg gaccgtcatt ctgaagcgcc agttccttag ggtccgctgt
     5341 tgttcggaga gcaccgcccg cgttcggcca aacacgaagt tccgtcaact ccacctgccc
     5401 cgccaggtta tcgggtttga gaacgggccc ctcaaaggtg gcctgccccg caatgagccc
     5461 ttcaaagggc aacgggcctt tctgtccggg ccgcagttgt tggattgtgg agaagctgag
     5521 aggtgtgaat cggatatgac cttttcccgg gtaatcgccg gcgagccgga actcggcgtt
     5581 accctctact ttcgatctgc gcagcacggc gttcgtctgt accgtaagcc ggtcatcttt
     5641 cgacgtggcc agcagccgta tattgcccac acgctctttc tcgaacatca ggtcgcgaat
     5701 cgagacctca ccattcagtg agccgagacg aaaagctccg ttcacgatat cggcggaccc
     5761 ttcggccctg gccccgatgg cggcttgaac ctgcggcccc aacctctgaa ccgtcttgaa
     5821 ttgccccagc gtgaacccct ttgttgacgt ttgaaagtgc atcttgccgt tcttccaatc
     5881 gtcctcgggg tgatcgtacg ctcccacgag atcgaggcgc gcggcaccga gctgtaactg
     5941 gccggcgatc agctccacac gcccgcccgt cacacgcagg tcggcccgca cactgtcgaa
     6001 ggtttcctcc caggcacgaa aattctgaat ggtggctttg atagttgcat cgggtgagcc
     6061 atacgtcccc gacacatcca cggagccgcg aagagtgccg tcaaccggtt ccttccggcc
     6121 tatctccgcg agcagcgtcc cgagttgtgc cttctcgaga gacgcacttg cacggatcgt
     6181 gctatccggc gccagcctcc agttttcgag tccggcttca ccctgtgcta ccgccaccat
     6241 ttctccgcgc cggacggaaa gcttatctac gccggccaga tcgttctcga tcttaaatgt
     6301 cgcggcgagc cggtcgaacc gctgattctg aactacaaag ccggtcaatt gcagccgtcc
     6361 ggtgatttgg ggggcgttca gtttgccgag cacattgccg ctgaattccg ctgtgccacc
     6421 ggcagccaat tctaccggca attgctccgg ggccttttcg cttaccatat gaatggccgg
     6481 aagaaggtcc gtcaagtcgc gggaaagaag atgtacccga aggttttcgc caagcgtccc
     6541 gctgaaatcg attcttgtat cgcccgcttc gatggaggaa tctccaagac gcaaggttcc
     6601 ggtgcgctgg ttataagcga cttcaacgga cccgtgaacc gggttcggtc cctcggcggg
     6661 agacacttta aatccacccg ttacttcgac gccgcgcaac cctccgccgg tcaacgttcc
     6721 actgacacgc accgggccgg acaccactcc gctccaggca agactcggcc ggttcacaac
     6781 ctgggtcaac tcgcttaaca ggaatccgtt gatctctccg gccacgcgca aacgttcgaa
     6841 ctcttcgagg cccagactcc cgttgaacac accgcccagg gccgcggtat gcaggccgtc
     6901 caagcgaagc ccctgcggcg acacgcgcag gtccgcttgg aacgccgccc gaggaatgtt
     6961 gacccccgcc cggcggaagc caagatcgcg ccccgtgagt ttgccggtta caagatatcc
     7021 cgcctcgctg gaccagctcg cgtgtccgtc acgaatcgct acggttcccc gtcgctcgac
     7081 aggcagcttg aactggccgg ccagttcagg aaccgacaga tccgccacga agttgaggcc
     7141 ggcctgcggt acgcggaaat cctgaacagt tcccgagagc gtgatggagg tctggcccat
     7201 tccgagccgt gctttggaaa tttccagcct gtcccgctcc atcgccagcg tggcatccag
     7261 ggcgaatgca accggcgaga atctgggtgt gtcgacgttc aggcgggaga actccacgtt
     7321 cccttcatag cgcggaacgg cggggttgta ggtgaaatga gcgttcaggt cctctccgcg
     7381 cgcgctgatc gggacggttc gcgagtcata agtaatctcg ccatcgttca gccggaaatc
     7441 ccgcacagcc agatccaata tcgtctcgat gggactcttc gtcgaagtgc gcttcacctt
     7501 tggttccgga atgttcgttt tgccgttctc atcgacgagt atgtggatgc ggggctggtc
     7561 cactatcagc gaagcgatat cgacgtcctt cctgaggagt gagacgatct tcaaacccac
     7621 ttcgatctgc ccggcgcgga acaaaggagg cgcatccggc ggctcggtcc catgcagcac
     7681 gaatccgcgg accctggcgg taagggtttt ccaatcgatt tggaacgaac caatttgtga
     7741 actcccgccg ctggcccgct ccacttcata gacgatgcga tcccgaactt tgttgtagaa
     7801 ccactcggac tgaagtacga agaaggcagc tatggagatg ccaacgacaa gcacgatgat
     7861 cgcgccgccg gcccagaaca ggatcttagc cgggcgcctc acgaaaagac ctcgtcccga
     7921 aactcaatat ccacctgcag ccgcgtttcc tgtagccagc gttccagcga ttgatccacg
     7981 cgctggttcg tcaggatttt ctcgatatcg cctcgaactt cgtcgagcgg cgggattctt
     8041 tccgcatggt tctgtccggc ctgtggcaag aaaaccgttt catagtacgt ctcgatgtcg
     8101 cgtgcgggaa cctgtatggc cggacgaaag cggtattcga tgaagcgtag cgtagtgagc
     8161 tggagcagaa gatgatcgcg aacatcgtct tccctgattt cgtattcggc caggctactc
     8221 cggaaagcgt cctcgttcgc gaaacgcccc ttgatctgat cgagcaggtg gttggcctct
     8281 acaaactggg cgggcggata acggtttgcg tccatctccc tgcggatcag agcgcgctcc
     8341 accaggcgtt cggcggcctt gcgcatcgta tccggattca gttcggcggg tctgccgtca
     8401 aggaatgccg tgattctaag atcccgcacg atctcgcttt gagtgatgac tgtagcgccc
     8461 actctcacgg caatgcgatc gatgagttct ccccccgccc cgtaggacgc aaccggcaac
     8521 agcagcaatg cgcaaacagc gtgcttcatc aaaacgcctg tccgagagag aagtggaact
     8581 gaaagcggct gagccgctga agcgtccgcg gacattgagg acccggaccc gcggcacagt
     8641 tgaacagaag ctcctcgcga gtcccctcaa aaccccaaaa ccggggcgaa ttcggaacgt
     8701 acgcaagatc cacgcggacg ggacccacag gcgtacgata ccgtattccg aaacccaccg
     8761 catgcaccat gtaatcgaag ttggaatcgt ccttctgtgt ctggcgcagc gacagccgcc
     8821 tcaaactcga atagacgttt cccgcttcgt gaaagagtac gcccgtcagg ttttcgccga
     8881 cgagcgggta tcttagctcc agggtgttca tcaggagcgc tttgccccca atcgggaagc
     8941 cggttgttaa atcgcggccg ccggcctggt tctcggaaaa cccgcggtgt gaggaagcgc
     9001 caccggcaaa gaaccgctcg ggaagaggga tatcctgtag cggatcgtcc tccgatgcaa
     9061 aactgtacat ccacccgaag gtagtttgac gggcaaagac aacttgccgg gtcagcggat
     9121 gataagtcgc gttccgcgcc agcaaccggg aaaaatcggt ctgcgaaccc aaaaatttgg
     9181 cggtaactcc caggtcgacg gtgttgtaga taccacgtct cgcatcgagc gggtcgtcgc
     9241 ggcggtcctg aatgaagctg ccgccgacaa cggtaacacg aaccggctgc gagaacagag
     9301 gaatcagacc aggcgtgatc ttcagcgtgt tttcatccac gctcacacgc cggatttgaa
     9361 tgcggtactg cgcggtgttc gccttggaaa gcctgtgacc cacctgggcg cttgcttcca
     9421 tgcgctttgc cgcaaatgtc ctcacatcgc gtgagtcgtc gtatatgccg gtgaaattga
     9481 gattgatgtc ctgattccct ttgaactgcg gtgcaaggta gttgattaca cccctgcgct
     9541 gaaaagagga aaagcgcgta ttcaggctga cggtgtgagc cacacccaga acgttcgaac
     9601 ggctgatacc aatggagatg cgagggctga accccgccga gccgcccggc gatgtgagat
     9661 tcgtcgccgc gccgcctcca atgcgggcga tttcggcgcc cacaccggcg ttcagggaat
     9721 acttccgcgc ctcctctact tcgtaaagta cgttcttgtt gcgctccttg ccttcggagt
     9781 tctgaacggc cgtatccacc ttggcgaaga tgccgagctc gtacaggttc cgctggctct
     9841 cattcattgc gatctgggag agggcatcgc caggccgcag gagaatgcgt tcccgcacaa
     9901 gctcgggatc ggtggtttca agccccccca ccagcacctg ccggatgaaa taacgcggac
     9961 cctcattaat aatgaacttg agctgcatct ggtggggacc gggtcccgga gtggctgtga
    10021 aatcgaagct cgcgtcgggg aagccgttgt tgtagtaaaa gctcaggatc gcgtcgcggt
    10081 cggcggcgat gttgatctca ctatacggct gcccttcgct cgaatgcagc agcgactgga
    10141 tgtattcgta gtcgcgaaga ttgaccccgc tgatttcgag cgccgacaca aaccactgcg
    10201 gcccctcttc aatctgaagg tataccgcta tgtcgttcgg atcgcctccg tggtttgctt
    10261 caattcgcga ggaaaccttt acttcccgaa aaccattgcc tcgatagaga tccgtgatcg
    10321 cctggcggtc acgctccagg tattcctcgc tatagcgccc gtgccggaac tgaagaaggc
    10381 tggcggtgcg caagtacatg cgctcgcgga ttgtgccggt gtcgaaatag gagttgccgg
    10441 cgatttccag gtgcttgaga ctgtgccgtg gtccgcggtc gagcacaaac gtgattactt
    10501 ctttatccgt gttgggaccg gcggtcttaa aatcgacccg ggcatcgaag tacccctcgg
    10561 attgcaaata ggattgtaag ttgccggcac cctccaccaa caagtccttg tcaacggact
    10621 gctcctggta gacaggtacc aactggcgta gtttgcctcc cgagatcttc gcgccttccg
    10681 tacggatttc gattgttggt ccggcatcga ttttcagtac gggggtgacg gtgttgggat
    10741 cgggccggaa cttgagatcg tccagggtga cgctggccaa caagcggtcg tctttgcgat
    10801 aggcagtccg gatgttttcg agcccacgct gaaggcggtt ctccgtaacg cttttccatc
    10861 cgagcaggcc ccatagcctt ttccaacttg tggcgccgac gatgtcctcc ggagggcgct
    10921 caagatcgcc ggtaagcgtc ggatgggcga atttcgctcg ttcccccggc tcgataaaga
    10981 accggatatt cacctggttc acttcgggct gcgcctcgaa gtccgactcg attctgggcc
    11041 ggtaaaaccc attgctctta agaagctgct ccagtttctc gattgcctgg cggaaagcgc
    11101 gctccgtgaa ctgggagccg agttccagct tggtcgcgtt ctcgagttgt ccccggttcg
    11161 gcggatccgg aacaccctga acggttacac ggccaatgaa ccacgccggc ttcgtgagga
    11221 accgcaagac aacaccgccc tcgcgcaact ccgcttcca
//
LOCUS       DASTMK010000120         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_614642, whole genome shotgun sequence.
ACCESSION   DASTMK010000120 DASTMK010000000
VERSION     DASTMK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614642"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFQ79_05000"
     CDS             <1..526
                     /locus_tag="VFQ79_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0139041.1"
                     /translation="SLRDNPDFSNANARRRRSESRSALPVGLGTGIRLLHHYCIIYAT
                     VYKGTGFPVASTQWGRWGTLICYDRQMPETARILAVKGAQLILVPAWGSYSELNDIMM
                     RVRAYENGVWLAFVHPKRCLVIDPRGRIIAQNDREQDQIVTAQISTAKVRRVLLERRR
                     PEIYGEILGQAKEK"
     gene            528..2507
                     /locus_tag="VFQ79_05005"
     CDS             528..2507
                     /locus_tag="VFQ79_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0139042.1"
                     /translation="MTVVCPSFRQCAFAAVAFLLLSASQGVGQTSPRQVGRLLDPAIQ
                     TPDAIAVEMQRYLAARVPELRVPPTAEEFTREASRVRRRLLDEVVLHGWPKEWVESPV
                     KVEDLGLVPSGKNYRMRKLRFEVFPGFQTTGILYEPETVQGRIPAILNVHGHVGAEGN
                     AVEYKQKICINQALRGILALSLEWLGSGELNHAENQHRYAAHLDLVGANGVGVFYLTM
                     RKALDYLYQHPTVDRSRIGMTGVSGGGWQTILLGALDERVFAAVPAAGYAPYRYRLAR
                     IQEAGDHEQNPTDLFRGVDYTHLTALLAPRPALLIYNAEDNCCWRASLVKPELFDLVA
                     PIFRLYRGEDRFFWHENIDPGDHNYHLDNRLRAYRFFSSSFGLPEITQEIPVDAQIKS
                     VEELRVDLPPGNATLITLARTLAQRIERAPIPSRTSERAAWADRQRKGLAETVRFPQF
                     ILSHAWGVASTKHSGLETISYKFEFSNGLTATAVRVKAMTTPDNAPVTIVLNDKGKKA
                     SGAPVSDRVNRGEIVLAADLLLTGDMTPGPRPGPGGYAQLLATFGERALGLEAAQLVT
                     LSRWLSERSQDRTLRLEATGVRSQVVSLVATALHPALFSDVVIREGIPTLGQLLEVPT
                     EPRAAPELFCLGLYRDFDIDRLIAMGAPARISSQR"
     gene            3013..3366
                     /locus_tag="VFQ79_05010"
     CDS             3013..3366
                     /locus_tag="VFQ79_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139043.1"
                     /translation="MPWKTEVQAQLANSFALAVTVAHYPTGASNWNPIEHRLFSEISR
                     KWAGEPLDSYQEILNYARTTRTQTGLKVTAYLDRRHYPCGVKPTPEQIVSLRLHRHET
                     LPHWNYTIKLNVNRP"
BASE COUNT          723 a         1117 c         1069 g          708 t
ORIGIN      
        1 ctcattgaga gacaaccccg acttcagcaa cgccaatgcg cgccgacgcc ggtctgaaag
       61 cagatccgcg ctacccgtcg gtcttggcac aggaattcgt ctcctgcacc attattgcat
      121 tatttatgca acggtctata agggtacggg gtttccggtg gcatcgacac agtggggccg
      181 gtgggggacg ctcatctgtt acgaccggca gatgccggag acggcccgga tccttgcggt
      241 caagggcgct caattgatcc tggttcccgc atggggctcc tacagcgagc tgaacgacat
      301 catgatgcgc gtgcgagcgt acgagaacgg cgtttggctg gccttcgtgc atccgaaacg
      361 ctgtctggtg attgacccga gaggccgcat tatcgcgcag aacgataggg aacaggacca
      421 aatcgtcacc gcgcagatca gcaccgccaa ggtccggcga gtgctgctcg agcggcgacg
      481 gccggaaata tacggcgaaa ttctcggcca ggcaaaggag aaatgagatg accgtcgtct
      541 gtccttcttt ccggcaatgc gcctttgcgg ccgtcgcatt cctccttcta tccgcatcgc
      601 aaggggtcgg ccaaaccagt ccgaggcagg tcggccgcct tctcgatccc gcgattcaaa
      661 cgccggacgc tatcgccgtc gagatgcagc gttatttggc ggccagggtc cccgagttgc
      721 gcgtgcctcc aaccgccgaa gagtttaccc gcgaggcgag tcgggtgagg cggcgcctgc
      781 tcgatgaagt tgtgcttcac ggatggccga aggagtgggt ggagtcaccg gtgaaggtgg
      841 aagatctggg cttggttccc tcgggcaaga actatcgcat gcgcaagctc cgcttcgagg
      901 tcttcccagg attccagacg acagggattc tttacgagcc ggagaccgtc caagggcgga
      961 ttccggcgat tctaaacgtc catggacacg tcggcgcaga aggtaacgcg gtcgagtaca
     1021 agcagaagat ctgcatcaat caggcgcttc gggggatatt ggccctgagc ctggaatggc
     1081 tcggcagcgg cgaactgaat cacgccgaga atcagcaccg gtacgctgcg cacctcgacc
     1141 tggtgggcgc caacggagtc ggcgttttct atctcacgat gcggaaagcg ctcgactacc
     1201 tttaccaaca ccccactgtg gaccgcagcc ggatcggcat gactggggtt tccggcggcg
     1261 gctggcagac gatccttctg ggcgccctgg acgagcgcgt gttcgccgca gtcccagccg
     1321 ccggctacgc accgtatcgg taccgccttg ctcgaattca ggaggccggc gaccacgagc
     1381 agaacccgac cgacttgttt cggggagtcg attatacgca cttgaccgcc ttgctcgcgc
     1441 cgcgaccggc cctgctcatt tacaacgcgg aggacaactg ctgctggcgg gcctccttgg
     1501 tgaagcccga gttgtttgac ctggtcgcgc caatctttcg tctctatcgc ggcgaggatc
     1561 gctttttttg gcacgagaat atcgacccgg gggaccacaa ctaccatctg gacaaccggc
     1621 tccgggccta ccggttcttc tccagcagtt tcggcctgcc ggaaatcacg caggagatcc
     1681 ccgtggacgc acagatcaag agtgtggagg agttacgagt cgacctgccg ccggggaacg
     1741 ctacgttgat cacgctggcg cggacgctcg cacagcgcat cgagagagcc cccataccgt
     1801 cccggacttc ggagcgtgct gcctgggctg atcggcagcg caaaggtctt gcagagacag
     1861 tccgatttcc ccagttcatc ctgagtcatg cgtggggagt cgccagtacc aagcactccg
     1921 gcctcgagac gatctcgtac aagttcgagt tctccaacgg gctgacggcg acggccgttc
     1981 gcgttaaggc aatgacaact ccagacaatg caccggtcac aattgtgctt aacgacaagg
     2041 gcaagaaggc ttcgggagcc cccgtgtccg accgtgtgaa ccgcggggag atcgttctgg
     2101 cggccgattt gctccttacc ggcgacatga cgccggggcc gagacccgga ccgggaggct
     2161 acgcgcagct tctggcaact ttcggtgaac gagctctggg cctggaggcg gcccaactgg
     2221 tcacgctcag ccggtggctc agcgagcgtt cacaagaccg caccctccgc ctggaagcca
     2281 ctggtgttcg gagccaggtc gtgagtttgg tggccacagc gttacacccg gcgctctttt
     2341 ccgatgtggt cattcgcgaa gggatcccca cgctgggcca actgctggag gtgccgacag
     2401 aacctcgggc ggcgcccgaa ctcttctgcc tgggtcttta tcgggacttc gacatcgacc
     2461 gcctcatcgc catgggcgcg ccagcgagaa tttcctccca gcggtaagaa ataacggttg
     2521 taaatctggg gcccgacatt ggtggttttt gaggccccgg gccgggtgcg gccttcgccg
     2581 ccaccccatt gtgggatccg gagactcaaa cgctggtatt ctcatttcat tccatggcgg
     2641 ctcgaccgcc gtggcggatc agcgccgctg gctctacagg tcacagtaga gcgcctgtgg
     2701 agcaataaca aagtgcggtc tgtgttcagc aatctgctgc tggttggcgg cgtgatttac
     2761 atggcgcggg gatcctacgg tccgggcttc ctgacgtctg ccgacgtcaa aaccgctttc
     2821 tgaatgccaa cttactccag gcggatggaa agctgatcat cctcgatgaa gacggcaggc
     2881 tcgtgcttgc gaggcccaaa ccagacagta gcctcgaaat cctctcaaag gcacacgtgc
     2941 tcagccgcaa tgcctggacc atcccgacgc tcgcggggac cactcccggc ggcagcaaca
     3001 gttgccgatg ctatgccctg gaaaaccgag gtccaggccc aactggccaa ctctttcgct
     3061 ctcgccgtga cggttgccca ttatccgact ggcgcttcca actggaatcc catcgagcat
     3121 cgcctcttct ccgaaatctc gcgcaaatgg gcgggcgaac ccctcgattc ctaccaggag
     3181 attctcaact acgcacgcac gacgagaacc caaaccggcc tgaaagtcac ggcctacctc
     3241 gaccgccgcc actacccgtg tggtgtcaag cccaccccag aacaaatcgt gtcactccga
     3301 ctgcaccgtc acgaaacgct tccgcactgg aactacacca tcaaactcaa tgtgaatagg
     3361 ccttagcgta caaaattccc ggaatggtcc ctcctggcct acatcaaatg tgcccaggcg
     3421 tggtcgaagc cagagatcct ggttgggtcg cacgctctat cggattcgac gatcgcaggc
     3481 cgaatcccac ctagccccaa tactgttcac ttaatgattt tgatagatgc ggagaagtca
     3541 gttttgattg tcgactgcgg cattcgcggc cgcaacggat agttgctcat ctttcgcttc
     3601 acacctcgcg ggttgat
//
LOCUS       DASTMK010000121         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_453940, whole genome shotgun sequence.
ACCESSION   DASTMK010000121 DASTMK010000000
VERSION     DASTMK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453940"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFQ79_05015"
     CDS             complement(<1..538)
                     /locus_tag="VFQ79_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU0139044.1"
                     /translation="MDPQRQTVFLSYNRLDKPAVEAIAGKLQDREIETWLDRWNLIAG
                     EPWQEAVERALTDCETCAVFVGPSGQGPWQNNEMRAALDLRMKAGGTGRRVIPVLLPR
                     AKEESIPLFLAGNTFVQFESLEDEEALYQLLCGIKGVMPGRRDTPSRQPVRGQCPYRG
                     LDAFDIADGPLFFGRNALV"
     gene            596..2047
                     /locus_tag="VFQ79_05020"
     CDS             596..2047
                     /locus_tag="VFQ79_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139045.1"
                     /translation="MKTVRRLKQAVVGLLFVAAFTMNSAAAQESGNLMVGWASVSITP
                     DKPVALAGQMHTRVSKSVRDPVTATVLALETKGSAADQAILISCDLVGIVPEIEEELR
                     RRVRERLPDFDSNKLFLNATHTHTAPETRDGRYQIPSGAMQPAEYRAFAVDRIASAVF
                     EAWSGRKPGGVSWALGHAVVGHNRRAIYQDGSARMYGKTDGPDFRALEGYEDHGLEML
                     FLWDAQDKLTGIAINIACPSQVLESQLYVSADFWHDVRKLIKDQFPENPFVFPMTGAA
                     GDQSPHFLFRRRAEERLRERLGVSQTEEIARRIVNAVNYVLPAARKDIRHAVPFVHKV
                     EELSLPVRKITVEEADAAKQEYERLSKLPETERSRYVLLNRSRNVMDRYEKQKEEPYY
                     KMKLHAIRLGDIAIATNPFELFLDYGIQIKARSQAEQTFVVQLAGGGTYLPTAKAVAG
                     GHYGAEAASNLVGPEGGQVLVDRTVDAINSMWK"
     gene            complement(2028..>2966)
                     /locus_tag="VFQ79_05025"
     CDS             complement(2028..>2966)
                     /locus_tag="VFQ79_05025"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU0139046.1"
                     /translation="EWSAPFELADTPNFPDTNCTMFVDSKKSLWLFWPVILANEWETA
                     LMKYKRSSNYNQAGPPRWDDAGNVLIIPKNMVERMQEVWKQDLKPTPFGRYATRLVAM
                     ASDKLTSRLGWFTRTHPLELPSGRILLPLYSDGFSFSLMAISDDGGETWFGSEPIVGY
                     GNIQPSVVRKKDGTLVAYMRDNGPPPKCIHISVSKDEGVSWSPAQDTELPNPGASVEA
                     IALRDGRWIIVYNDLPSGRHSLAVSMSDDEGATWKWTRHMEQKPEGRYHYPSVIQAQD
                     GSVHVTYSYFVREGGKEVKSIKHARFEPSWITSTSN"
BASE COUNT          620 a          891 c          882 g          573 t
ORIGIN      
        1 gcaccagggc gtttcgccca aaaaacagcg gtccgtcggc gatgtcgaac gcatcgagcc
       61 cccggtaggg gcactggccg cgcaccggtt gccgggatgg agtatcgcgg cgcccgggca
      121 ttacgccttt gatgccgcag agcagttgat acagggcttc ttcgtcttcg agcgactcga
      181 actgcacgaa ggtgttcccg gcaagaaaca gaggaatgga ttcctctttg gctcgtggca
      241 gcaggacggg aattactcgc cgcccggtcc cgccggcttt catacgcaga tcgagggcgg
      301 cacgcatttc gttgttttgc cacggaccct gcccggaagg acccacaaac acggcgcacg
      361 tctcgcaatc ggtcaacgcc cgctccaccg cttcctgcca cggctccccc gcgatcaggt
      421 tccatcggtc aagccacgtt tcgatttcgc gatcctgaag ctttcccgca atggcctcca
      481 cggcgggctt gtccagacgg ttgtaggaca agaacaccgt ctgcctctga ggatccatgg
      541 tggctatgat atccgaagtg cgatttcact ccgcgctcaa tataatctgt gaggaatgaa
      601 aacggtgcga agacttaagc aagcggtggt tggcctgttg ttcgtggccg cgttcacaat
      661 gaattcagcc gcggcgcagg aaagcggcaa cctgatggtg ggttgggctt cggtgagcat
      721 tacgcctgac aagcccgtcg ccctggccgg acagatgcac acgcgcgtgt cgaaatccgt
      781 tcgcgatccg gtgacggcta ccgtcctggc gctcgaaaca aagggttctg ccgcggacca
      841 ggcgatcctg atctcctgcg atcttgtcgg catcgttccc gaaatcgagg aagaactgcg
      901 gcgccgcgtc cgggagcgat tgccggattt cgatagcaac aagctcttcc tgaatgcgac
      961 ccatacacac acagctcccg aaacaaggga cggccgttac cagattccgt ccggagcgat
     1021 gcagcccgcc gagtaccgcg cattcgcggt ggaccggatc gcatccgccg ttttcgaagc
     1081 atggagcggc cggaaaccgg gcggtgtgag ctgggcgctt ggccatgccg tcgtgggaca
     1141 taaccgccgc gcgatctatc aggacggctc ggcccggatg tacggaaaaa cagacggccc
     1201 ggactttcgc gccctggaag gttatgaaga tcacggactg gaaatgctgt tcttgtggga
     1261 tgcacaggac aagctgaccg gaatcgccat caacatcgcg tgcccatcgc aggtgctgga
     1321 aagccagctt tacgtctccg ccgatttctg gcacgacgtg cgaaagctga tcaaagacca
     1381 gttccccgaa aatccgtttg tgtttcccat gactggtgcg gcaggggacc agtcgcccca
     1441 cttcctgttc cgccgccggg cggaagagag gctgcgcgaa cggctcgggg tatcgcaaac
     1501 ggaagagatc gcccgcagaa ttgtgaacgc cgtaaactac gttctgcccg cggcacgcaa
     1561 agatatccgt catgccgtcc ccttcgtgca caaagtcgaa gagttaagtc tgcccgtgcg
     1621 gaagattacc gtggaggaag cagacgcggc aaaacaggaa tacgagcggc tctcgaaact
     1681 gccggagacc gaacgatcgc gatacgtcct gctgaatcgc agcaggaatg tgatggaccg
     1741 ctacgagaag cagaaggaag aaccctatta caagatgaag cttcacgcga tccggctcgg
     1801 cgacatcgcg atcgccacca atccatttga gctgtttctc gactacggga ttcagatcaa
     1861 agctcgcagc caagccgagc agaccttcgt ggtgcaactc gcgggcggag gcacgtacct
     1921 gcccaccgcg aaggcggttg cgggcgggca ctatggcgcg gaagccgcca gcaacctggt
     1981 aggtccggaa ggtggacaag ttctggtcga ccgcaccgtg gatgcgatca attcgatgtg
     2041 gaagtgatcc aggacggttc gaaccgggcg tgcttgatgg acttgacctc tttgccgccc
     2101 tcccggacga agtagctgta ggtcacgtgc accgatccgt cctgcgcctg aatgacggaa
     2161 gggtagtggt atcgcccctc cggtttctgt tccatgtggc gcgtccattt ccacgttgca
     2221 ccttcgtcgt cggacatcga aaccgccagc gagtgccggc ccgaaggcag atcgttgtac
     2281 acaataatcc aacgcccatc gcgcagcgca atggcctcga cgcttgcgcc cgggttggga
     2341 agctctgtat cctgcgcggg actccagctc accccttcgt cctttgaaac acttatgtga
     2401 atacacttcg gtggagggcc gttgtcccgc atgtaagcca ccagcgtccc atccttcttg
     2461 cggaccaccg agggctgaat attgccgtag ccgacaatcg gctcgctgcc gaaccacgtc
     2521 tctccgccat cgtcgctgat ggccatcaaa gagaacgaga agccatccga atacagcggc
     2581 agcagaatac ggccggaggg aagctccaac ggatgcgttc gcgtgaacca gccgaggcgg
     2641 gatgtcagct tgtccgatgc catcgcgacc agccgtgtgg cgtagcggcc aaagggagtc
     2701 ggcttcaggt cctgcttcca tacctcctgc attctctcca ccatattttt cggaattatg
     2761 aggacgttgc cggcatcgtc ccagcgcggc ggtcccgcct ggttgtaatt cgacgaccgc
     2821 ttgtacttca tcagcgctgt ctcccactcg ttggcgagaa tgacgggcca aaaaagccag
     2881 aggctcttct tcgaatccac aaacatggtg cagttggtgt cggggaaatt gggtgtgtca
     2941 gccagttcga acggagcgct ccattc
//
LOCUS       DASTMK010000122         4717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_70506, whole genome shotgun sequence.
ACCESSION   DASTMK010000122 DASTMK010000000
VERSION     DASTMK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70506"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VFQ79_05030"
     CDS             <1..1189
                     /locus_tag="VFQ79_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139047.1"
                     /translation="IIRAGGAIVDALEWVFAEVKAEIHDLWAEVIEGFRAAGRAISDV
                     FHWALKKGKDVLEVIVAVWKKIGQAVDEVLEWAKSVAGFAWDVFAGIVTRVKYSVEYI
                     LSWLEDDFLPGMRQFIAGLLKIGYKVVDLVSYMAGKTAIVVAEIVAAVFQAGVVFGDF
                     IAATIDHPDDVLENLLRAAKEYGKTVKEMVSAAVEQAVEDMKKRTIDNLRDIGYSAVE
                     VVKAAAEIGGGAVATVVTIVLQWFGDYRPLTAEEMTDATSVYGKSIDLDKVQVAVLSP
                     PVDLVEYLNGERPFTTMYLLNFASWDEVKRDTLIHELTHVWQGLVEGPFYMLQALHAQ
                     MGEGYDYGGTTGLQNAGGDFTKFNREQQASIIQDYFRIRFEQGNPESSYIDYVPYAQA
                     VFA"
     gene            complement(1258..1821)
                     /locus_tag="VFQ79_05035"
     CDS             complement(1258..1821)
                     /locus_tag="VFQ79_05035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0139048.1"
                     /translation="MSHKKTIAAIALACVLAIAPAAWAQSAPTRSPDVVFVPTPQEVV
                     DAMLSLAQVGKGDTVYDLGCGDGRIVITAAKKFGANAVGIDIDPERIQEANANARREG
                     VTGKVSFRQEDLFQADIKSASVVTLYLLSTLNQKLKPKLLAELKPGTRIVSHAFDMGD
                     WKPEKESDVNGRRIYLWRVPAKTASAR"
     gene            complement(1954..2904)
                     /locus_tag="VFQ79_05040"
     CDS             complement(1954..2904)
                     /locus_tag="VFQ79_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0139049.1"
                     /translation="MPVDADVNVVIGASGGLGKAVVHELVRQGKPVRAVSRSGKAPLP
                     DSVEVQMADVSDTGMARRVCDGARVVYLCANPSYQHWVIQLPRIMTAVIDAAGRSGAR
                     LVFGDNLYMYGPVGVPMREDLPSNAEGPKGRLRAEIAQMLLYAHRAGKVRATIGRASD
                     FFGPGVVNALMGERVFRPALAGGTVWIAGNPDVPHTYSYIEDVACALVILGERTEALG
                     EVWHTPNPETVTTRRFLEMIFEKAGQRPKIRSARRWLVKTMGLFSRQMQEMEEVLYQF
                     ERPFVVDHSKFGRTFGAEATPLAEAIGRTLDWCRTHPPAT"
     gene            complement(3074..>4717)
                     /locus_tag="VFQ79_05045"
     CDS             complement(3074..>4717)
                     /locus_tag="VFQ79_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139050.1"
                     /translation="GAELTALLLGIPGGQMDVTGSYAEQTSNFSFYVHDDWKISRNLT
                     LNLGLRYEYESPITERYNRSVAGFAFDTANPLDAAARENYAKIAIPERPVDQYRLMGG
                     LTFAGVGGNSRQYWKGESNNLMPRLGFAYQLTPLTVLRGGFGMFYSSMGILTSSSIQT
                     GFSQSTPIQASLDSGLSFIATTANPFPNGLQEPLGAAGGLMTNIGQAVSFFPEERKHP
                     YTQKWSFGVQRQLPAQYLIDISYVGSKSVRLGVDRNLSFTPAEYLSTLPYRDEKTIEY
                     LSATFPSPLAGLHPIYGANTSRAGLLDPYPHFGNVTILDQPVGYSWYHSMQTRFEKRF
                     SRGYTFQLSYTWAKAMEAVEYLNASDPMPYESISSMDRTHRLVASGIYELPFGRGRAL
                     GSNWHPVTNFILGGWQLSGVVQRQSGAPLGFGNAIFNGDLKDIVLPKSERSVDRWFNT
                     EAGFNRNSAQQLANNIRAFPLRFSGIRNDGQARWDFSAIKNFKVTEKATMQFRAECFN
                     AWNHPNLSGPNTTPTNSNFGTITGQNPPRSWQGALKLTF"
BASE COUNT          983 a         1403 c         1360 g          971 t
ORIGIN      
        1 catcattcgc gccggtgggg ctatcgtgga tgccctggag tgggtgttcg ccgaagtgaa
       61 ggcggaaata cacgatttat gggcggaagt tatcgaagga ttccgcgccg ccggacgtgc
      121 catctcggac gtgttccatt gggcgctcaa aaaaggtaaa gacgtcctgg aagtgatcgt
      181 cgcggtctgg aaaaagatcg gccaggctgt cgatgaggtg ctcgagtggg ccaaatccgt
      241 tgccggattc gcctgggatg tctttgccgg tatcgtcacc cgagtgaaat attccgtcga
      301 gtacattctc tcgtggctgg aggacgactt cctgcccgga atgcgccaat tcatcgccgg
      361 cctgctgaaa atcgggtaca aagtcgtcga cctggttagc tacatggccg gaaaaaccgc
      421 aatcgtcgtc gccgagatag tagccgcggt gtttcaggcg ggagtcgtgt tcggcgattt
      481 cattgcagcc accatcgacc accccgacga cgttctggag aacctgcttc gagccgctaa
      541 agagtatggc aaaaccgtga aggaaatggt tagcgcggcg gtggagcaag ccgtcgaaga
      601 tatgaagaag cggacgatcg acaacctccg ggatatcggc tactcggcag tggaggtcgt
      661 caaggcagcg gcggagattg gcgggggagc cgtcgccacg gtggtcacca tcgtgctcca
      721 atggttcggc gactaccggc cgctaacggc agaagaaatg accgatgcca caagcgtcta
      781 cggcaaatcg atcgacctgg acaaagtaca ggtggccgtt ctttcgcctc cagtcgacct
      841 ggtcgaatac ctcaacggcg agcgcccgtt cacaaccatg tacctgctga acttcgcttc
      901 ctgggacgag gtgaagcggg atactctgat tcacgaactc acccacgtat ggcagggact
      961 ggtggaaggg cccttctaca tgctccaggc actacatgcg caaatgggag aaggttacga
     1021 ctacggcgga accaccggat tacagaacgc cggtggcgac ttcaccaaat tcaatcgcga
     1081 acagcaagcc tcaataattc aggattactt ccggatacgc ttcgagcaag gtaaccctga
     1141 aagcagctat atcgattacg tgccttacgc acaggccgtt ttcgcatagg ctgttcgctg
     1201 aagggatgcg tgcgcccgtt cctacggacg cacgcaccgc aacgattctg ctctctctca
     1261 gcgtgccgag gcggttttgg ccggaacgcg ccacagatag attctgcgtc cgttcacatc
     1321 agattccttc tcgggcttcc agtctcccat gtcgaacgcg tgcgatacga tgcgagtgcc
     1381 gggcttcagt tcggccaaca gcttgggctt caatttctga ttcagcgtac tcagaagata
     1441 aagcgttacg acacttgcgc tcttaatgtc ggcctggaac agatcttcct gacggaaact
     1501 gactttgccg gtgacgcctt cacggcgcgc gttggcattt gcttcctgaa ttcgctccgg
     1561 atcgatatcg atccctaccg cattcgctcc gaacttcttc gccgcggtga tgacgatgcg
     1621 cccgtccccg cagcccaggt cgtaaacggt atctcccttt cctacctgcg ccagcgacaa
     1681 catggcatcg accacttcct gaggagttgg gacgaacact acatccggcg accgcgtggg
     1741 cgcactctgg gcccacgccg ccggagcgat ggccagtacg cacgccagcg cgatcgcagc
     1801 gatcgttttc ttatgtgaca tatacttaat ctcctgcctc ctgcccggac gccgcggtct
     1861 ccatgaccgt agccgggtgc tcagattaga tttagcgcgg cggaatgcgt ttcgtcaaat
     1921 ctcgtattgg ctcgtattgg ctccatcccg gcgttatgta gctggcggat gggtccggca
     1981 ccagtcgagc gtccgcccaa tggcctctgc caaaggtgtc gcctctgcgc cgaatgtacg
     2041 tccgaatttg gaatggtcca cgacgaacgg acgttcgaat tgatacaaga cctcctccat
     2101 ctcctgcatt tgcctgctga ataaccccat cgtttttacg agccaccggc gcgcggagcg
     2161 aatcttaggc ctctggcccg ccttctcgaa gatcatttcc aagaaacgcc gcgtcgtgac
     2221 ggtttcggga ttcggggtgt gccagacttc gcccagcgcc tccgtacgtt cccccagaat
     2281 aacgagcgcg caggccacgt cttcgatata ggaatacgtg tgcggcacgt ccgggtttcc
     2341 ggcgatccag acggtgccgc cggccagcgc gggacggaaa acacgctctc ccatcaatgc
     2401 gttcaccaca cccggtccaa aaaaatcgga agcgcggccg atcgtcgcac gtactttccc
     2461 cgcccggtgc gcatagagaa gcatctgcgc gatttccgcc cgcagccgcc cttttggccc
     2521 ctccgcgtta ctgggcaggt cctcccgcat cggcacgccc accggtccgt acatgtagag
     2581 gttgtcacca aataccagcc tcgccccgga ccggcccgcg gcatcgatta ccgccgtcat
     2641 gatccggggg agttgaataa cccaatgctg gtaggaggga ttggcgcaga ggtaaacaac
     2701 ccgggctcca tcgcagacac ggcgcgccat gccggtgtcc gaaacgtcgg ccatttgcac
     2761 ttcgacagag tccggcagcg gcgctttgcc cgacctgctc acagcgcgaa ccggtttgcc
     2821 ctgacgcacc aactcgtgaa ccacggcttt ccccaaacct ccgctcgctc cgataacgac
     2881 gtttacatcc gcatcaaccg gcataagatt cattctgcat cagcgcttga gcgcagacgt
     2941 aaaagccccg ctccgggcac attccgggag cggggcaagc tacaaccgtg ttgccgattc
     3001 tagtccgctc cgtgaccgtc gcggctcaga aacaaaggct ttactgagcc gcgcgtgtga
     3061 gcaagcgggc tcattaaaag gtcaatttga gagcaccctg ccaggaccgt ggaggattct
     3121 gtccggtgat ggtgccgaaa ttggaattgg tcggtgtggt attcggaccg ctcaggttcg
     3181 ggtggttcca ggcattgaag cactcggcgc gaaactgcat agtggccttc tcggtcactt
     3241 tgaagttttt gatggccgag aaatcccacc gcgcctgccc gtcattccgg atgccgctga
     3301 agcgtagcgg gaacgcccgg atattgttcg caagctgctg ggcggagttg cggttgaaac
     3361 cggcttcagt attgaaccac cggtccacgc tccgttcgct cttcggcagc acaatgtcct
     3421 tcaggtcgcc gttgaagatg gcattgccga aaccgagtgg cgcaccgctt tgccgctgca
     3481 caacaccgct caactgccag ccgccgagga tgaaattcgt caccggatgc cagttggagc
     3541 cgagggcgcg tccgcggccg aacggcaatt cataaatacc gctcgccacc aggcggtgcg
     3601 tgcgatccat gctggagatg gattcatagg gcatggggtc ggaagcgttc aggtactcga
     3661 cggcttccat cgcttttgcc cacgtgtagg acaactggaa cgtgtagcct ctcgagaatc
     3721 gcttttcgaa gcgcgtttgc atagagtgat accaggagta gcccaccggc tggtccaata
     3781 tcgtgacatt accgaagtgc ggatatgggt ccagcagtcc cgcacggctg gtgttggctc
     3841 cataaatcgg atgcagcccg gccagcgggc ttggaaatgt agcgctcagg tactcgatgg
     3901 tcttttcatc ccgatagggc agagtgctca ggtattcagc cggcgtaaag ctcagattac
     3961 gatcaacgcc gagccggact gacttgctgc ctacgtagga aatgtcgatc aggtactgcg
     4021 ccggcaactg ccgctgaaca ccgaacgacc acttctgcgt gtagggatgc ttcctctcct
     4081 cgggaaagaa gctgactgcc tgcccgatat tcgtcattag cccgccagcc gctccgagcg
     4141 gttcctgcaa gccattcgga aacggattgg cagtggtggc tatgaaactc agtccgctgt
     4201 cgagcgacgc ctgaatggga gtggactggc tgaaaccggt ttgaatcgaa ctggaggtga
     4261 gaattcccat ggaggaataa aacatcccga atcctccccg taacacggtc aggggcgtca
     4321 gttggtacgc gaagccgagc cgcggcatca ggttgttgct ttcgcccttc cagtactggc
     4381 gcgaattgcc accgacaccg gcgaacgtca agccacccat cagtcggtac tggtccaccg
     4441 gacgctccgg aatggcgatc ttcgcataat tctcccgcgc ggcggcatcc agcgggtttg
     4501 ccgtatcgaa cgcgaaaccg gccacggacc ggttgtagcg ctcggttatg ggagactcat
     4561 actcgtaacg caggccaagg ttgagcgtaa gatttcgcga gatcttccaa tcgtcgtgaa
     4621 cataaaacga gaaattggag gtctgttcgg cgtagctgcc cgtcacgtcc atctggccgc
     4681 ccggaatgcc gagcaacagg gcggtcaact ccgcgcc
//
LOCUS       DASTMK010000123         6448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_70511, whole genome shotgun sequence.
ACCESSION   DASTMK010000123 DASTMK010000000
VERSION     DASTMK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70511"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..338)
                     /locus_tag="VFQ79_05050"
     CDS             complement(21..338)
                     /locus_tag="VFQ79_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139051.1"
                     /translation="MNIRVHGGQGLSLDEMGAFLQSSSELSFQGQSRQEIYGWVDQTL
                     RQQHYERLGRTERGLIRQYVAIEFRIIQLHFMLLMFWTCESSGLTLGHGGSARTTSGL
                     PGS"
     gene            468..1730
                     /locus_tag="VFQ79_05055"
     CDS             468..1730
                     /locus_tag="VFQ79_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139052.1"
                     /translation="MPNPGDNTHPLRSESRRHQPPMLDRRDLFRAAGVGALAMTAGSY
                     SRIMGANDRVQLGLIGCGGRGRGVMGTFIKTDQVDVKAVCDVYADRIDRAKQQAPNAS
                     SFTDHRKLLESKDLDAVLIATPDHWHAAITIDALNAGKDVYVEKPLTLRIEEGPPIVK
                     AARVNNRICQVGMQQRSGQIYIQARDEYIKPGKLGKITLARTWWHGNGAHLMKAPPAL
                     KTKPSNLDWARYLGPVKWREWDPQQYWNFRAYLDFGGGQITDLFTHWIDVVHMFMEQD
                     NPTSAVASGGVYCYKDGRTAPDTICVLLGYPAEWTATFEATLTPGITGAAVEMCGTEG
                     RLNITRSRFEFHSAEKGAPPVIVKSPREQTIDHVENFLDCMRTRKLPNGDVYIGHRSA
                     QASHLGNVAYLEKRRINFDPDREEILPL"
     gene            complement(1746..2180)
                     /locus_tag="VFQ79_05060"
     CDS             complement(1746..2180)
                     /locus_tag="VFQ79_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU0139053.1"
                     /translation="MIQFTHEVRVDTRGKGLYEFTTEVEFWVRSKRVSMGLLTIYVRH
                     TSASLTIQENADSDVIYDLSKFFERLVPEDNRLYRHTVEGPDDMPAHIRAALTQTQLS
                     IPVANGKPVLGTWQGIYLFEHRAAPHRRSVVLHFIGEAQEAG"
     gene            complement(2177..4030)
                     /gene="glmS"
                     /locus_tag="VFQ79_05065"
     CDS             complement(2177..4030)
                     /gene="glmS"
                     /locus_tag="VFQ79_05065"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU0139054.1"
                     /translation="MCGIVGYIGNRKAVPIILDGLRRLEYRGYDSAGLAVIGDGNELC
                     LRRASGKLRNLEESVRMHPIEGVFGIGHTRWATHGRPTEENAHPHRDCKGDIVVVHNG
                     IIENYLSLKHQLRGEGHTFVTETDTEVIAHLIEKHYTGDLEEAVRKAVKQLSGVFALA
                     VIARSDLNKIVVARMGPPAVIGLGDGEYFVASDVPAILNHTRDMFFLADGDMAVLTRE
                     GVHIKDFDGRPVNRQVSHILWDPIMAEKGGYKHFMLKEIYEQPRAVRDTILGRVGRES
                     GRVFLDEMDITEREFREFHNVRIVACGTSWHAALAGKFMIERLARIPVEVDYGSEFRY
                     RDPILDAGTLTVVISQSGETADTLAAQREAKSKKSKTLAICNVIGSMITREAAGTLLT
                     HAGPEIGVASTKAFTCQLTALFILAMYLGQQRQTIDPETSRFLVQELLTIPAKLETVL
                     TRDAIYEDLVRVLFRSTDFLFLGRGIHFPIALEGALKLKEISYIHAEGYPAGEMKHGP
                     NALIDEKLPVVVLATCDRDVEASRLRYEKTLSNIQEVKAREGLVIAVASDGDEQVSKI
                     ADDVLWVPPSNELLLPILEIVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(4033..4281)
                     /gene="purS"
                     /locus_tag="VFQ79_05070"
     CDS             complement(4033..4281)
                     /gene="purS"
                     /locus_tag="VFQ79_05070"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU0139055.1"
                     /translation="MKARVYVSLKPTVLDPQGQTVRSALSGLGHSSVSEVRQGKFFEL
                     TLNTQAAPDAVKAEIEQIAHDVLANPVIEEYRVEILER"
     gene            4401..6179
                     /locus_tag="VFQ79_05075"
     CDS             4401..6179
                     /locus_tag="VFQ79_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0139056.1"
                     /translation="MKRRSFLQAAAVSGTSMLAAAARPNILWIIGEDFSPDLGCYGNS
                     LVHTPHLDRLASEGVRFSHAFVTAPVCSASRSAIATGIYQTSIGAHHHRSHRTDGYRL
                     PAPVQVFTEYFRKAGYHTSNVVTAATGLRGTGKTDFNFHAERVFDGTDWQQRKPGQPF
                     YAQVNFPETHRAFRPSPERHIDRAKAIPPPYYPDHPAAREEWAVYLETAQNLDANAGK
                     VLDRLRDEGLLEETIVFFFGDHGRPMPRGKQFLYDEGIRIPLIVRIPEKFQPPGFRPG
                     SVRDDLVSAIDITATSLKLAGIEPPGHMQGRPFLGANTKPREYVFAARDRCDETSDRI
                     RAVRDARFKYIRNYHPDRAWTQPNNYKDTAYPMLRLLRDLDRTGKLNAAQARFMAAKR
                     PVEELYDTHSDPYELRNLADSPEHARMLERMRGVLKEWIASTGDKGEIPEQPAAVEDT
                     SYRTQVDGWCTRAFAAATRTGEALTVVCKGKTNSILRSCVTEGGDMVLEFRARSAAIR
                     PQMVFWGTIDKMVNQPDPENGAKLEFRPDGKWHDYSVPFRAAGFLGVIGLDLGPGEGS
                     IEFDSIRLLRKTGSKTEIVERWDFAV"
BASE COUNT         1325 a         1973 c         1861 g         1289 t
ORIGIN      
        1 cggcttgctt gcgccgttgc tcaagagcct ggaagtcccg acgttgtccg cgccgaacca
       61 ccatgcccca gtgtaaggcc actgctctcg cacgtccaga acataagcag catgaaatgt
      121 aactgtatta tgcgaaactc aatagccacg tactggcgga tcagcccccg ctccgtccgg
      181 cccaaccgct cgtagtgctg ctgccgcaac gtctggtcca cccatccata gatctcctgc
      241 cggctctggc cctggaagct cagttcgctg ctgctctgca aaaatgcccc catctcgtcc
      301 aggctcaacc cctgtccacc gtgcactcgg atgttcaatc cccagcatcg ccaatcctct
      361 cttcaggctc atcttgtgtg agaaacgcag ccccgcttca ggctcatctt gtatgagata
      421 agactgggat ctgcttgaag acccggacag tgacataatc gtgttctatg ccgaaccccg
      481 gagacaacac ccacccgctg cgttcagaat cccggcgcca ccaacccccg atgctcgacc
      541 ggcgcgatct ctttcgcgcc gccggcgttg gcgcactcgc tatgacggcc ggctcgtatt
      601 cgcgcatcat gggcgccaat gatcgggtgc aacttggcct gatcggctgc ggcggacgtg
      661 gtcggggtgt catgggtaca ttcatcaaaa ccgaccaggt ggatgtgaaa gccgtctgcg
      721 acgtctacgc cgaccgcatc gatcgcgcca aacagcaggc gcccaatgcc agcagtttca
      781 ccgatcaccg caaacttctc gagtcgaaag acctggatgc cgttctgatc gccactcccg
      841 atcactggca cgcggccatc acgatcgatg cattgaatgc cggcaaagac gtgtatgtcg
      901 aaaaaccgct gacgcttcga attgaagaag ggccgccgat tgtaaaggcg gcacgcgtca
      961 ataaccgcat ttgtcaggtc ggcatgcagc agcggtccgg ccagatttac atacaggcgc
     1021 gcgacgaata catcaagccg ggcaaactcg gcaagatcac gttggcacga acatggtggc
     1081 acggaaacgg cgcgcacctg atgaaggcgc caccggcgct caagacaaag ccttccaacc
     1141 tcgattgggc gcgctatctg ggtccggtga agtggcggga gtgggacccg cagcaatatt
     1201 ggaactttcg cgcttatctt gatttcggcg gcgggcagat taccgattta ttcacgcact
     1261 ggatcgatgt tgtccacatg ttcatggagc aggacaatcc tacttcggct gtcgcctccg
     1321 gcggtgttta ctgctacaag gacggccgca ccgctcccga taccatttgc gtgctgctgg
     1381 gctatccggc ggaatggacg gccactttcg aagccacatt gacgcctgga atcactggcg
     1441 ccgcggtgga aatgtgcgga accgaagggc gcttgaatat cacacgaagc cgctttgaat
     1501 tccattccgc ggaaaaaggg gctccgccgg tcatcgtgaa gtcgccgcgc gagcagacca
     1561 tcgatcacgt ggagaacttc ctagattgca tgcgcacgcg caagctgccc aacggcgacg
     1621 tctatatcgg gcatcggtcc gcgcaggcat cgcacctcgg caacgtggcc tatctcgaaa
     1681 agcggcgcat caatttcgat ccggatcgcg aagagattct gccgctgtaa gcgcgccgtt
     1741 gtgtgctatc cggcttcctg cgcctcgccg ataaagtgca gcacgacact ccggcggtgc
     1801 ggagccgcgc gatgctcgaa gagatagatg ccctgccagg ttcccagcac gggctttccg
     1861 ttggccaccg ggatggagag ttgagtctgt gtcagggccg cgcggatgtg agcgggcatg
     1921 tcgtccggcc cttcgacagt gtgcctatag agccggttgt cctcgggaac cagccgctcg
     1981 aaaaacttgg agagatcgta gatcacatcg ctgtcggcgt tctcttgaat ggtgagggag
     2041 gccgatgtgt ggcggacata aatcgtcagc agtcccatgg atacgcgctt cgagcggacc
     2101 cagaactcca cttccgtcgt aaactcgtac aacccctttc cccgggtgtc gacccgaact
     2161 tcgtgcgtaa actggatcat tcgacggtaa cactcttagc gagatttcga ggctgatcca
     2221 cgtcgcagcc acggcgaacg gcaatatggt aagcgagcag ttgcaacggg acaatttcaa
     2281 ggatgggaag cagcaactcg tttgatggag gcacccagag gacgtcatcc gcgatcttgg
     2341 atacctgctc gtctccatcg gaagcaacgg cgatcaccag gccctcccgc gccttgacct
     2401 cctgaatatt ggaaagtgtt ttttcgtatc gaagccggct cgcctccacg tcgcgatcgc
     2461 aggtggccag taccaccacc ggaagctttt cgtcgatcag cgcattcggt ccgtgtttca
     2521 tttccccggc cggatatcct tccgcgtgga tataggagat ctccttcagc ttgagcgcgc
     2581 cctccaatgc gatcgggaaa tgaatgccac ggccgagaaa caggaagtcc gtcgagcgga
     2641 acagaacgcg caccaggtct tcgtaaatgg catcgcgcgt cagtacggtc tccagtttcg
     2701 ctggaatcgt cagcagttcc tgcaccagga acctggatgt ctccggatcg atcgtctgcc
     2761 gttgctggcc caggtacatg gccagtatga acagtgcggt caactggcac gtaaaggctt
     2821 tcgttgacgc cactccgatc tccggaccgg cgtgcgtgag cagcgttccc gccgcctcgc
     2881 gtgtgatcat cgacccgatg acgttgcaga ttgcaagggt cttggatttt ttggacttgg
     2941 cctcccgctg cgcggcgagt gtgtccgcgg tctcgccgga ttgggagatg acgaccgtca
     3001 gcgttcccgc atcaagtatc ggatcgcgat accggaactc actaccatag tccacttcaa
     3061 ctggaatgcg ggccagccgc tcgatcatga acttaccggc cagggctgcg tgccaactgg
     3121 tgccgcaagc gacgatccga acgttgtgga actcgcggaa ctcccgttcg gtgatgtcca
     3181 tctcgtcgag gaatacacgg cccgactccc gccccacacg gccaagaatg gtatcgcgga
     3241 cggcgcgcgg ctgctcataa atttccttca gcatgaaatg cttgtagccg cctttttccg
     3301 ccatgatggg atcccacaaa atatgcgaca cctggcgatt cacggggcgc ccgtcgaaat
     3361 ccttgatgtg aacgccttcg cgcgtgagca cagccatgtc gccgtcggcg aggaagaaca
     3421 tatcccgggt gtgattcaga atggccggca cgtcggaggc aacgaaatac tctccgtcgc
     3481 ccaggccgat caccgccggc gggcccatac gtgccaccac gattttattc aggtcggagc
     3541 gtgctataac agccagcgca aacacgccgg atagttgctt caccgccttg cggaccgctt
     3601 cctccagatc gcccgtgtaa tgcttctcga tgagatgggc gatcacctcc gtatcggttt
     3661 ccgtaacgaa agtatggcct tcgccgcgga gctgatgctt gagcgagagg taattctcaa
     3721 tgattccgtt gtggacgaca acgatatcgc ctttgcagtc ccgatgtgga tgagcgttct
     3781 cttcggtcgg gcgcccgtgt gtggcccacc tggtatgccc gatgccgaac accccttcga
     3841 tcggatgcat ccgcacagac tcttcgaggt tgcgaagctt accggatgcc cggcgcaagc
     3901 acaactcgtt gccgtcgccg attaccgcca gaccggcgga atcgtagccc cgatattcga
     3961 gccgccgaag cccatcgagg atgatgggga cggccttcct gttaccgatg tatccgacaa
     4021 tgccacacat aactaccgct ccagtatttc cacccgatat tcctcaatca ccgggttggc
     4081 aaggacatcg tgcgcaattt gttcgatctc ggccttaaca gcgtccgggg ccgcctgcgt
     4141 gttcaacgtg agttcgaaaa acttaccttg cctcacctcg gagacgctgc tgtggcccag
     4201 tccggagagc gcggaccgta cggtttgacc ctgcggatcc aacaccgtcg gtttcaaaga
     4261 aacgtatacg cgagctttca tggattctcc tattctagac ttacccgcgg tccggaaccc
     4321 ggttccggct gtcgacggcc accggccggg ccggcgcgaa tcgttcagtt cgggctgaag
     4381 gtagaatatt aggtcattgg atgaaacgac gatcattttt acaggcggcg gccgtatcgg
     4441 gcacttccat gctggccgcg gcagcacggc ccaacattct ctggattatt ggtgaggatt
     4501 tcagccccga tctcgggtgc tatggcaaca gcctggtcca cacgccgcat ctggaccggc
     4561 tggcttccga gggcgtccgg ttctcgcacg cttttgtgac tgcgccggtc tgctcggcgt
     4621 cgcgctcggc gatcgccacc ggaatatatc agacgagcat cggggcgcac caccatcgca
     4681 gccaccggac agacggctat cggctgcctg cgcctgttca ggtgttcacg gaatacttcc
     4741 gcaaggcggg ctatcacact tccaacgtcg tcacggccgc cacaggtttg cgcggcacgg
     4801 gtaaaaccga tttcaatttt catgccgagc gagtcttcga cggaaccgac tggcaacaac
     4861 gcaagccggg gcagccgttc tatgcgcagg tgaactttcc ggagacccac cgcgcgttcc
     4921 gtccctctcc ggagcggcac atcgaccgcg cgaaggcaat cccaccgccc tactaccctg
     4981 accaccccgc ggcgcgcgag gagtgggcgg tgtacctgga aacggcccag aatctggacg
     5041 ccaatgccgg caaggtcctg gaccgtctcc gcgacgaagg cctgctggaa gagacgatcg
     5101 tcttcttctt cggcgaccat ggccggccga tgccgcgcgg caagcaattc ctttatgacg
     5161 aaggaatcag aattccgctc atcgttcgta ttccggagaa gttccagccg ccgggcttcc
     5221 ggcccggctc ggtgcgcgac gatcttgtga gcgctatcga tatcacggcg acgtcgctga
     5281 aactggccgg catcgagccg ccaggccaca tgcaaggccg cccttttctg ggagccaaca
     5341 ccaagccgcg tgagtacgta ttcgccgcgc gcgatcgctg tgatgagact tccgaccgca
     5401 ttcgcgcggt gcgcgacgca cgcttcaaat atatccgcaa ctaccatccg gaccgcgcct
     5461 ggacgcagcc caataactat aaggacacag cctacccgat gctgcggctg ctgcgcgatc
     5521 tcgatcgtac gggcaaactc aatgcggccc aggcacgctt tatggcagcg aaacgtccgg
     5581 tagaggagtt atacgacacc cactccgatc cctacgaact gcggaacctt gcggattcgc
     5641 cggagcacgc caggatgctc gagcgtatgc gcggggtgtt gaaagaatgg attgcgtcta
     5701 ccggtgacaa aggtgagatt ccggaacagc cggccgcggt agaggacacc agctaccgga
     5761 cgcaagtgga cggctggtgc acgcgcgcct ttgctgctgc gaccaggaca ggcgaagcgc
     5821 tgacggtagt ttgcaaagga aaaacgaaca gcattctgcg gtcgtgtgtg acggaaggtg
     5881 gtgacatggt gctggagttt cgtgcgcggt cggccgctat tcgtccacag atggtgttct
     5941 ggggaaccat cgacaagatg gtcaatcaac ccgacccgga gaacggagcg aagctggagt
     6001 tccgccccga tgggaaatgg cacgattact ccgtgccctt ccgtgccgcc gggtttctgg
     6061 gggtcatcgg attggacctc ggtcctggcg aaggaagtat cgaattcgac tccatccggc
     6121 tcctgcgcaa gactggctcc aagacggaaa tcgtggagcg atgggatttc gccgtataag
     6181 gttcagcgtt cggtcacgcg tacagcgccg cccgccgtac caaggaactc gggatccgta
     6241 tcgccgccgg tacctcacac ggaaaagtgg ctcggctcat cccgctcgaa gcaacggctc
     6301 tcgttgcagt gcgctggagc ggaaccgaag atggttcgat cctggatctt cggggttgaa
     6361 caggaaacct gaaagaggaa atctgaattg agatacggaa cctgcgggat tattcttgtg
     6421 ctcgccgcct gtttcacgag cgaagcca
//
LOCUS       DASTMK010000124        15467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_454126, whole genome shotgun sequence.
ACCESSION   DASTMK010000124 DASTMK010000000
VERSION     DASTMK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15467
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454126"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..635)
                     /locus_tag="VFQ79_05080"
     CDS             complement(120..635)
                     /locus_tag="VFQ79_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0139057.1"
                     /translation="MVTKVAFGLMLAAVAFADDGSFVFAGLSLTPEPWNKEANLAKLE
                     RYVRQAAAMGAQVVATPEGFLEGYVGNRGRSRDLTREKYFPVGEELNGAMMTRIRGLA
                     QQLKIHLLVGFAERRQEKMYNSAVIFSPEGSVVLHYSKSHTADDEPFNTIDLCINNAT
                     VNAKKGEVSTA"
     gene            765..1898
                     /locus_tag="VFQ79_05085"
     CDS             765..1898
                     /locus_tag="VFQ79_05085"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0139058.1"
                     /translation="MSKVLFVGLDVHAETIAVAVAEQGGEVRSVGIIPNRLESVRKMV
                     HKFGPVQHLRACYEAGPTGYVLYWQLTQLGVDCQVIAPSLVPTKAGDRVKTDRRDAKK
                     LARCHRAGELTAVWVPTAEQEALRDLVRAREAAKKDQLKARHRLGKFLLRHGRQRPDE
                     IKAWTKKHREWIKSHVRFEQAALEATLADYVDEVDHVAERIVKLEKAIDEAIRQAPPE
                     LRAVIEALQALRGVAQTTAASIVSEVGSLSRFPTAPQLMGYSGLVASEHTSGNGVQRG
                     GITKTGNGHLRRVLVEAAWAYQHRPDVIGHLARRQKDLTLSDDVKNIAWKAQQRLHKR
                     YKAMAARGKNKNQIVAALGRELLGFIWAIGVKTEQQQKLAKAA"
     gene            complement(2295..3440)
                     /locus_tag="VFQ79_05090"
     CDS             complement(2295..3440)
                     /locus_tag="VFQ79_05090"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0139059.1"
                     /translation="MAQSSAAIVGTVRDASGAVVAGATVTVTNVGTGLSQTRSTSGDG
                     AFSFPLLPVGEYRLAVESPGFQRHVRTGIRLAVNDKPTIDVGMQLGQITESVTVTGSA
                     PLIEAQTGTLRGLVDQERITNLPLNGRDMVQLISVQAGAIKTADSSAGGEGVAYAVNG
                     SRQNGVYYLMDGGYNTSTYRNWSGTFPNPDAVQEFSVQRSNFTAEYANATGAVVNVVT
                     KSGTNEFHGSAFWFMRNAVLNARNFFAATRDSLKRNQYGATLGGPVVKDKLFFFAAYQ
                     GTTLQSDPRLSPQFLPTAAMRSGDFSAVGRTIRDPITGQPFPNNQVPVARASYVGNLG
                     RRLAYPEDINYARYVPGSSSVQNIQARRPYNRDYTRVLIWSDLSSPS"
     gene            3723..5183
                     /locus_tag="VFQ79_05095"
     CDS             3723..5183
                     /locus_tag="VFQ79_05095"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0139060.1"
                     /translation="MSPRCCAQSAVLLLAFLPLLAADPFDPVREFIRKGLVETGMPSI
                     SVAVAKNGEIVWEEGFGWADRERRIKATEHTMYSLASISKPFTATGLMVLVQQGKLDL
                     DHAVNNYLGNAIIRTRVGDGAQATVRRLANHSSGLPLHVQFFYSDTSRWPPSMDETIL
                     RYGNLVTAPGERFQYSNLGFGILDYIIERISRKSYADFMREQVFIPLGLTHTSVHIGP
                     GLESFASTRYGRDGLPVAFYEFDHTGASAVWSSAHDLARFGMFHLKAHLPDQKAILSD
                     EWINAMQKPTFPSGKNVGYGIAWVVTDTAGGYRTISHNGAMHGVATTLRLVPSEKLAV
                     IVLCNGSDQLPHQVADEIFKVMLPNWRQPERPSDSHPVYTVPSQLKGAWNGQIITYKQ
                     DLPLTLRLLDSHEVHAQLGDQKKVLVNDPRWEDGYFSGEFIGNLGTEDTGPGPVQIRL
                     LLKLRGDKLNGSATATALPGMRGGFNLTHWVELKRE"
     gene            5180..7306
                     /locus_tag="VFQ79_05100"
     CDS             5180..7306
                     /locus_tag="VFQ79_05100"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0139061.1"
                     /translation="MNTSMKAIFCVLPVVLAVGVAAQEFRALDPRLHGAPEQMLSSYL
                     QQIAAQQLEERRHRVDSVRSKEDYEQRKTRLRTTALRILGGLNEPRTPLNLRRTGTLD
                     RGDYRVEKIVYESRPRYYVTANLYIPQKGNGPFPAILHPLGHSITGKNRAFYQRLSIG
                     LVKQGFVVLTYDPIGQGERRIFWDADLADSKVGGPTQEHSMVGWQSLLAGETVARHRI
                     WDGIRSVDLLESLKEVDQNRIGVTGCSGGGTLTTYIAALDDRLKAAAPACYISSWDEQ
                     LKGTGPQDAEQQFPDQLREGLNHSDWIGLAAPKPYLIVSTDQDFFPLEGARKTFAEMK
                     RIYELYDAAVKVEWFHEPGGHGTPTASREAIYAWMNRWLRGEPGPVKEPSMVFEHEED
                     LNATRTGQVATSLGGETASTDNIRRFRDRLPPRPEKPDAARVRAEILRLTRYKPATVP
                     LNLSRGAAIDRSGHRIEMLTYRSEEGLIVPAALVTPAKPRAGRVALLVDSRGKSAALA
                     ADGDVLQLAQLGYTVLAIDPAGIGETAFQRHAAAPWSSPQLTFLALMVGRPLIGIRMN
                     DILRGIDALKELNIGMTEGVLGVARGYIGTTLLHAAAIDSRLSRLIVEGNLVSYQAVG
                     AAPIHRNIDDLVLPGVLGRYDLPDLVAAVAPRPVALRNLISPAGRALFRAEMQDAYAY
                     PLRVANADVGLRNGNEKLTDAYPFLR"
     gene            complement(7427..10588)
                     /locus_tag="VFQ79_05105"
     CDS             complement(7427..10588)
                     /locus_tag="VFQ79_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139062.1"
                     /translation="MCSVPAFAQFTTASLNGVVFDATGAAVPEAKVTIHNTETGFEAS
                     SVTGVSGLFVFPRLPVGNYRLTVEKPGFATYLQEGIVLTVNQSATQNVALRVGQVSER
                     VVVSANVELVTTGTATVGQLIDERRVIDLPLNGRRAQSLLYLSAGTVDLDQSRSLGYA
                     GVYPGEQLANVNGTGPGQVNYQLDGAGHNDTYLNLNLPFPNPDAIQEFALQSDNLSAQ
                     YGNAAGGVVNIVTKSGTNEIHGTAFEFLRNGKLNARNFFAPRQDSLKRNQFGGSIGGP
                     IKRDRLFFFGTYQGTPIRTAPEGRIAFVPTEAQRRGDFSSISRQLIDPVTKQPFPGNI
                     IPESRLSPVARYVVDRIPLPNGSGQQITFTGGAANSDEHQWMAKLNYNAGKHQLDGRY
                     FFTDFNRPGDFSETNLLIASGNVMGVRVQNVAVSYTFTATPSLLFSTWFGWNSQTGGQ
                     KPNSTFGFPEAGMKIAAPPEREVDFSVDGYFSVTTSQPGKNDRSDWTIREDVTKIRGA
                     HEWHFGGEVVRIRNTMASNWLQSGNFTFADQLSGNNLADFMLGRVSAFTQGAGEFKDM
                     TGTKLSLYIQDNWRATPRLTLNLGLRWDPSLPFVEEKGRVACFVPGAKSARYPNAPVG
                     LVYGGPDPDARCPSRGYESNLANFGPRVGFAYRLTNDGKTSLRGGAGIYYTPFLSALV
                     YMHVDPPFGPIYDFEDVSFDDPWGSLGTPSPFPAQFGANIPGPEAEFTTPVSLYYMQR
                     DLRIPQIASWNLTLERQLIGSWVVRASYVGNKGTYLSGSDDYNPAQEMNPAIYVPGQS
                     TRGNTQQRRLYSDFSGVSAIISGHNSNYHSAQLTVEKRFSQGFSLLASHTWAKAIDDF
                     GWANPFNRRFDYGISRDDIAHSFKLSSIWEAPRLNLGGPAGAILNGWGVTGNVLWRGG
                     FPFGVRSGVDNSFSGVSRNRADFMGTDINDADLGSGRPHAEMISRFFDTSLFQQNAEG
                     TFGNSGKNVLRGPRHFNADLGILKNTRVNERVNVQFRAEFFNIFNNVNFRLPNATLTS
                     PAVGRITTAFDSRIIQFGLKVAF"
     gene            10845..12134
                     /locus_tag="VFQ79_05110"
     CDS             10845..12134
                     /locus_tag="VFQ79_05110"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139063.1"
                     /translation="MEIAGAGSLAVPLTGTPTPVRVGVIGIGRRGTGLLRILLNIPGV
                     QVPAVCDTNADNLAAAAASVEKKGQKPPEQYGGGPEAFRKLLARDDLQAVVIATPWEW
                     HAPMAVAAMKAGKYAGVEVPAAVTLEECWELVSTSEETGLPCMLLENDCFGDGALMAL
                     NLVEQGVLGEIVHCEGGYQHDIRAGILRAGQLSWRARHALKRNGDLYPTHPAGPMAWW
                     SSINRGDRFAYLTSTASKSRGINHYIKKYYGADHPHANAKFALGDVVTTVLKSENGVT
                     MVISHDTSLPRPYSDSGETSIPLMVIRLQGTEGIFSGSLDRIYIEGRNGPNSKMHKWQ
                     DIQPYYQRYRHQLWKNLGAEAAGYEHGGEDYVCLSQFVEAVRRRKPTPVDVYDAACWS
                     AISPLSEQSVAARSAPVEFPDFTRGKWKTPRRVEFLL"
     gene            complement(12347..12433)
                     /locus_tag="VFQ79_05115"
     tRNA            complement(12347..12433)
                     /locus_tag="VFQ79_05115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12397..12399),aa:Leu,seq:gag)
     gene            complement(12448..12834)
                     /gene="secG"
                     /locus_tag="VFQ79_05120"
     CDS             complement(12448..12834)
                     /gene="secG"
                     /locus_tag="VFQ79_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU0139064.1"
                     /translation="MVILFTILHVIVCLFLIVVVLLQSGKAADLAGAFGGMGSQTAFG
                     PRGAATVLSKATTIAATVFMLTSMSLSIMATRGSSTGGGGSVLERTAPATQQQTPAPG
                     TPAAPAQQPPAQQTAPPAQQPPADKK"
     gene            12970..13413
                     /locus_tag="VFQ79_05125"
     CDS             12970..13413
                     /locus_tag="VFQ79_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0139065.1"
                     /translation="MSTSSTDLAPGDYQALAEFRYQIRRFLQFSEHAARESGVEPQQH
                     QALLAIRGMKPETQPTIGEIAARLFLRHHSTVELVNRLEHGGLVARERHSEDGRQVLV
                     RLTPAGERVLRRLSIAHRSELQVTGPELARALQRLTRRNRKSEAA"
     gene            13410..15248
                     /locus_tag="VFQ79_05130"
     CDS             13410..15248
                     /locus_tag="VFQ79_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0139066.1"
                     /translation="MTPKEVCQSAEGHTRSVDELGDFTATPRMIPICLLAVVIGAGSS
                     FVALALLKLIGLFTNLFYFQRLSTELVPPAGNQLGWMAALVPVGGALIIGLMARYGSE
                     KIRGHGIPEAIESILINGSRVQPRLAILKPLSAAVSIGSGGPFGAEGPIIMTGGAFGS
                     MFAQFFRLSSAERKTLLVAGASAGMSATFAAPLSAVLLAVELLLFEWKPRSMIPVALA
                     SATAGAARRYLIGVGPLFPAPDHPVFAGIWVLVACAIAGVLAGLLSTILTAAVYAAED
                     AFQRLPMHWMWWPAIGGAVVGLGGLAFPQALGVGYETIGELVQGDVAKGTLAGILLVK
                     SVIWAVALGSGTSGGVLAPLLMMGGALGGLEAMFLPDAGLGFWPLISMGAILGGTMRS
                     PFTGVVFAIELTHDVNVLLPLLIASVVAYAVTVLLLKRSILTEKVSRRGFHLSREYSV
                     DPLEILFVREVMRTTVAAFPASATLDELAASFRPDHTPRGQHLYPVVDGGNRLIGVIT
                     RKDIVSLLARRARGTVAEFARTDPIVACAQEPLRAVVFRMAETGFTRMPVVDRTSGDR
                     LAGMVSLEDLLRARARNLTEERHRERVLRIRLPFSLAAKQASTGAD"
     gene            complement(15268..>15467)
                     /locus_tag="VFQ79_05135"
     CDS             complement(15268..>15467)
                     /locus_tag="VFQ79_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0139067.1"
                     /translation="PKQMAEIVDFLGMKFPLAIYLNFLLLHEVHHRGQLSAYLRPMGA
                     KVPDIYGGSADEPFQMAAEQP"
BASE COUNT         3161 a         4482 c         4728 g         3096 t
ORIGIN      
        1 gtccttggcc aagcgagatc tggccaacgg ttgccctcac aatgtggaag agttgatgga
       61 cgaccttgtc cgctccatcg aaagaatccg ccgatcgccc gccaaactcc gaggttgcat
      121 caagcagtcg aaacttcccc cttttttgcg ttaactgttg cattatttat gcatagatct
      181 atagtgttga aaggctcgtc gtccgccgta tgactcttgg agtaatgtaa aacgaccgag
      241 ccttcgggag aaaagatgac cgccgaattg tacatcttct cctgccgccg ttcggcgaag
      301 cccacgagta gatggatctt aagttgctgc gccagacctc gaatgcgagt catcatagcg
      361 ccattcaatt cttcgccgac cggaaagtac ttctcccggg tcaggtctcg tgaacgcccc
      421 ctgttgccga cgtagccctc caggaaccct tccggtgtgg cgacgacttg cgcccccatg
      481 gcggcggctt gccgcacgta gcgctccaat ttggcgagat tcgcctcctt gttccacggt
      541 tcaggagtca agcttagacc cgcaaagacg aacgacccgt cgtctgcaaa ggccacggcg
      601 gccagcatca atccaaatgc aactttggta accatttttg cccttgttgc caaacgccta
      661 gtcattatgg agcgaccagc cgccgattcg cagttgtcgc gggttacgct gcgaagaggt
      721 gaatccagcg gcgcagagcc gcatacgaaa ggaactggtc gctcatgtct aaagtactgt
      781 ttgttggtct ggatgtccac gccgaaacga tcgcagtggc agtcgcggaa caaggcgggg
      841 aggtccgctc ggtcggtatc atcccaaacc ggttggagtc ggttcggaag atggtccaca
      901 agtttgggcc ggtccagcat ctgagagcct gctacgaagc cggcccaacc gggtacgtgc
      961 tgtattggca gcttacgcag cttggcgtcg attgccaggt gatcgcgccc agcctcgtgc
     1021 cgactaaggc gggagaccgg gtcaagacgg accgccgcga cgcgaagaaa ctggcccgct
     1081 gtcatcgtgc cggggagttg acggcagttt gggtgccgac tgcggagcag gaggccttgc
     1141 gcgatctggt gcgcgcccga gaagcggcga agaaggatca gctaaaagcc cgccaccgac
     1201 tcgggaagtt cctgttgcgg cacggacggc agcggccaga cgaaatcaaa gcgtggacta
     1261 agaaacatcg agagtggatc aagagccatg ttcgattcga gcaagccgcg ctggaggcaa
     1321 ctctggccga ttacgtggat gaggtggatc acgttgcgga gcggattgtg aaactggaga
     1381 aggccatcga cgaggcaatc cggcaggcgc cgccggagct tcgagctgtc atcgaagctc
     1441 tgcaggccct ccgcggagtg gcgcagacga cggcagccag tatcgtctcc gaagtcggct
     1501 cgctgtcgcg cttcccgacc gctccgcagt tgatgggata cagcggcctg gtcgccagcg
     1561 agcacaccag cggcaatggg gtgcagcgcg gcggcatcac caagaccggc aacggtcacc
     1621 tcagacgggt gttggtggaa gcggcgtggg cataccaaca tcgaccggat gtcatcggtc
     1681 atctggcgcg tcgacaaaag gacttgacgc tcagtgacga tgtaaagaac attgcctgga
     1741 aggcccaaca gcggctgcac aaacgctaca aagccatggc agcgcgcggt aagaacaaga
     1801 accagatcgt cgcggcgctg ggacgcgaac tgcttgggtt catctgggct atcggagtga
     1861 aaacggagca gcagcagaag ctcgccaagg cggcgtaact cgctactgaa atgcactact
     1921 ggacagcaga gccgatgaca gcgcagggcc cacgaaaagg agaatcctcc tctcgttcta
     1981 tgcggtacgg tttccgaacc caacccgcgc acctagtgcg aggcagctcc cgacggatca
     2041 tgaccatgcg gttccgaccc gcgaatatca gagtgatcaa tcgtcgcgta tgctgctggg
     2101 ctgtcatcgg ccctgctgtc ccaacgagcc aaggggaacg aaacctacgg tttctttccc
     2161 ctttggaatc cccattcttc cctaaacgcg gctctgagcc caccagtggg aacacttcac
     2221 ggacacccgc aaaaagaaaa aggccggctt ccctcctgcg gaggaaagcc ggcctgaaaa
     2281 ccaaacccct caaattacga gggacttgac aggtcgctcc atatcagaac gcgcgtataa
     2341 tcccggttgt acgggcgtcg ggcttgaatg ttctgcaccg acgaactccc gggcacatac
     2401 cgtgcatagt ttatgtcctc cggatacgcc aatcggcggc ccagattacc cacgtaactg
     2461 gcgcgcgcga ctgggacttg gttgtttgga aaaggctggc ccgttatggg gtccctgatc
     2521 gtcctgccca ctgccgaaaa atcgcccgag cgcatggctg cggtcggaag gaactgggga
     2581 ctgagccgcg gatcgctctg caacgtggtg ccctgataag cggcgaaaaa gaacagcttg
     2641 tccttcacca cgggcccgcc gagggtcgct ccgtactggt tgcgtttcag agagtccctg
     2701 gtagcggcaa agaaattgcg tgcattcaaa acggcgttgc gcatgaacca gaaagcagag
     2761 ccatggaatt cattcgtgcc ggatttcgtc accacgttga cgacggcacc cgtagcgttt
     2821 gcgtactctg ccgtgaaatt gctgcgctgc acgctgaact cctgcacggc atcgggattc
     2881 gggaaagttc cgctccaatt ccgataggtg ctcgtgttgt agccgccatc catgaggtaa
     2941 tagacaccat tctggcggct gccattgaca gcataggcta ccccttctcc accagccgac
     3001 gaatccgccg tcttaattgc cccggcctgc acgctaatca attgcaccat gtcgcgtccg
     3061 ttcagcggca ggtttgtaat tctctcctgg tcaaccagtc cgcgcagcgt tcccgtttgt
     3121 gcctcgataa gcggtgcgga tccggtcacc gtaacggatt cggtgatctg gcccagttgc
     3181 ataccaacat cgatggtggg cttatcattc accgcgagcc ggataccggt acggacatgc
     3241 cgttgaaatc ccggcgattc gacagctagc ctgtattctc cgaccggcaa cagcggaaac
     3301 gagaaagcgc catccccgct ggttgagcgg gtctgcgaga gtccggtccc tacgttcgta
     3361 acggtgacgg ttgcgccggc gacgactgct cccgacgcat ctcttacggt tcccacgatt
     3421 gccgctgaag actgtgccac cgctacctct gggagaagca ggcacaagct gagcaacatg
     3481 gaaagataca aaactgatcg ccggtcgatt tgatttaagc tcacggcggg aacctccttg
     3541 agtgatttga agtatattac aacgttctca gcagggatgc agttcaggac tcgggctcgt
     3601 agtagtgccg gacttcacgc gcggcaaatg gaagaaccgc ggacctgtcg agatcccacg
     3661 gggtctgaca gtgccacatc ggagcacttc gataacggac gacagtggat atactaagcg
     3721 ggatgagccc gagatgttgt gcgcagtccg ccgttctgct gctcgctttc ctgccgctcc
     3781 tcgccgccga cccctttgat ccggtccgtg agttcatacg caaaggtctt gtcgaaaccg
     3841 gtatgccgtc catctctgtc gccgtcgcca aaaacggaga aatcgtctgg gaagaggggt
     3901 tcggctgggc tgaccgtgaa agacgcatta aagccactga gcacacgatg tactcgcttg
     3961 catcgatctc gaaacccttc acagccactg gcctgatggt gctcgtgcag cagggcaaac
     4021 tcgacctgga ccatgctgtc aacaattacc tcggcaacgc aattatacgc acacgagtgg
     4081 gcgatggcgc gcaagctacg gtgcggcggc ttgcgaatca ttcgtccgga ctgccgctgc
     4141 acgtccaatt tttctacagc gacacgtcgc ggtggccgcc gtcgatggat gaaaccatcc
     4201 tgcgatacgg caatctcgtg acggcgccag gcgaacgatt tcagtattct aacctcggct
     4261 tcggcatcct ggattacatc atcgagcgca tctcgcgcaa aagctatgcg gatttcatgc
     4321 gggagcaggt gttcatccct cttggactta cgcacacgtc ggttcacatc ggccccggtc
     4381 tcgagtcgtt tgcctcgacc cgatacggac gcgacggtct cccggtcgcc ttttacgaat
     4441 tcgaccatac cggggcctcc gctgtctggt ccagcgcgca cgatctcgcg cgctttggca
     4501 tgttccacct gaaagcgcac ctgccggatc aaaaagcgat tctttcggat gaatggatca
     4561 atgccatgca gaagccgacc ttcccgagcg gcaagaacgt aggctacggg atagcctggg
     4621 tggtgaccga cacggctggc ggttatcgca ccatctccca taatggagcc atgcacggtg
     4681 tcgcgacgac gctgcggctg gtaccttcgg aaaaactcgc ggttatcgtt ctgtgcaacg
     4741 gtagcgacca acttccgcac caggtggctg atgagatttt caaagtcatg ctgccgaatt
     4801 ggcggcagcc cgagcggccg tccgattcgc accctgtcta taccgtcccc tcccagctta
     4861 agggcgcttg gaacggacaa atcatcacct acaaacaaga cttgccgctc acgctccgcc
     4921 tgctcgattc tcatgaggtt catgcccagc tcggcgatca gaagaaggtg ctcgtcaacg
     4981 atccgcggtg ggaagatggc tacttcagtg gcgagtttat cggcaatctc ggaacggaag
     5041 acacaggtcc cgggcctgtg cagattcgcc tgctattgaa actccgtggc gacaagctca
     5101 acggttccgc aacagccacc gctctgccag gaatgcgagg aggcttcaac ctcacgcact
     5161 gggtggagct caagagagaa tgaatacttc catgaaagcg atcttttgtg ttctgccagt
     5221 cgtacttgcc gtgggcgtag cggctcagga gttccgagct ttggacccgc gtcttcacgg
     5281 cgctccggaa cagatgctga gttcctactt acagcagatt gcagcgcaac agttggaaga
     5341 gcggcggcac cgggtggact ccgtccggag caaggaagac tatgagcagc gaaagacgcg
     5401 gctgcggacc acagcgcttc ggatacttgg tggattgaac gagcccagga caccgctgaa
     5461 cttacgacgc acggggacgc tcgatcgtgg cgattaccgg gtagagaaga tcgtctacga
     5521 aagcaggcca cggtattacg taactgcgaa cctgtatatc ccgcagaagg ggaacggccc
     5581 gtttccggcg atcttgcatc cgctcgggca ctcgattacg ggcaagaatc gggcctttta
     5641 tcagcggctc tcgattggat tggtgaagca gggcttcgtc gtgctgacct atgatccgat
     5701 cggccaggga gagcggcgaa tcttttggga cgcggatctg gccgactcaa aagtcggtgg
     5761 accaacacag gaacacagta tggtgggctg gcaaagcctt ctggccggag agaccgttgc
     5821 acggcatcgg atttgggacg ggattcgcag cgtcgatttg ctggaatcac tgaaagaagt
     5881 agaccaaaat cgaatcggcg ttacgggatg ctctggaggc ggaaccctca caacctatat
     5941 cgcggcgctc gatgatcgac tgaaagccgc ggcgcccgca tgctacatca gttcgtggga
     6001 tgagcagtta aagggcacag gtccacaaga tgctgagcag caattcccgg atcaactgcg
     6061 ggaaggcttg aatcattccg actggatcgg gctggccgcg cccaagccgt atctgatcgt
     6121 gagtacagac caggattttt ttccgctgga aggggcaaga aaaacgttcg cggaaatgaa
     6181 aaggatctat gagctctacg atgccgccgt aaaagtggag tggttccacg agccgggtgg
     6241 acacggcaca ccgacggcaa gccgcgaagc catctatgca tggatgaatc gctggctgag
     6301 aggtgagccg ggcccggtca aggaaccatc gatggtgttc gagcatgaag aggatttgaa
     6361 cgcaacgcga acgggtcagg tggcgacatc gctgggcggc gagacagcga gtaccgataa
     6421 catacggcgt tttcgagata gattaccgcc gcggccggag aaaccggatg ccgctcgcgt
     6481 ccgtgcggaa atcttgaggc tcacacgata caagcctgcg accgtaccgc tgaacttgag
     6541 ccgcggtgcg gcaatcgacc gaagcgggca tcgaatcgag atgttgacct atcggagcga
     6601 ggagggcctg atcgtgccgg cagctctggt gacccccgcg aagccgcgtg cggggcgtgt
     6661 ggccctcctc gtggattcgc gaggtaagtc tgctgcgctt gcagcggacg gcgacgttct
     6721 ccaactcgca cagttgggct acacggtact tgcaatcgat cccgccggaa ttggagagac
     6781 ggcttttcag cggcatgccg cggcgccgtg gtcctcacca caactgacgt ttctggcgct
     6841 gatggtaggc cgcccattga ttggaattcg aatgaacgat atcctgcgcg gcatcgatgc
     6901 cttgaaggag ctcaatatcg gaatgacaga aggtgtgttg ggcgtggcgc gaggatacat
     6961 cgggacaacg ctgctgcacg cggcggcaat cgatagtcga ttgtcgcggc tgatcgttga
     7021 aggaaacctg gtttcatatc aggccgtcgg tgcagcaccc attcatcgca atattgatga
     7081 cctggttttg ccaggagtgc tcggccgata tgacttgccg gacctggtag cggcagtggc
     7141 tccgcgtccg gtggcgctgc gaaacctgat ctcaccggca ggacgagctc tatttcgggc
     7201 tgagatgcag gatgcgtacg cgtatccgct gcgggtggcg aacgcagacg tgggtttgcg
     7261 aaacgggaac gagaaactga ccgatgctta tccgttcctt cggtagtctg cccaaccgat
     7321 gtgctgctga gcgttcatgc ccgccccacc ggtaccagac tccaatcggg aggagaaagc
     7381 gaagccgcct gattgcgcct gattgctttt cgctaagcgc gtggattcag aacgccacct
     7441 tcaatccgaa ctggatgata cgcgaatcaa acgccgtggt gattcgtccc accgcgggag
     7501 atgtgagcgt ggcattcggc agacggaagt ttacgttatt gaaaatgttg aaaaactccg
     7561 cccgaaactg cacgtttacc cgctcgttga cccgcgtgtt cttcagaatg ccgagatcgg
     7621 cgttaaagtg cctgggacca cggagcacgt tctttccgga attgccgaag gtgccttccg
     7681 cgttttgctg gaacagagag gtatcgaaaa atcgcgagat catctccgca tggggccgtc
     7741 cggacccaag atccgcgtca ttgatatccg tgcccatgaa gtccgcccgg ttgcggctga
     7801 ctccgctgaa tgaattgtcc actccgctgc gtactccgaa cgggaatccg ccgcgccata
     7861 agacgttgcc ggtgacgccc cacccgttga gaatcgcgcc cgcggggccg ccgaggttca
     7921 ggcgcggggc ctcccagatg gacgagagct tgaaggaatg ggcgatgtcg tcgcgcgaga
     7981 tcccgtagtc gaaacggcga ttgaaggggt tggcccatcc gaaatcatca atcgctttgg
     8041 cccaggtgtg gcttgccaga agcgagaatc cctggctgaa gcgcttctct accgtgagct
     8101 gggcggaatg gtagttcgaa ttgtgccccg agatgatggc gctcacgccg ctaaagtcgg
     8161 agtaaaggcg ccgctgctgt gtgttgccgc gcgtagactg accgggaaca tagatggcag
     8221 ggttcatttc ctgagcaggg ttgtagtcgt ccgaaccgga aaggtaggtg cccttattcc
     8281 cgacatagga cgcgcggacg acccagctgc cgatcaattg ccgctccagg gtgaggttcc
     8341 aggaggcgat ctgggggatt cgcagatcgc gctgcatgta atacagggag accggtgtgg
     8401 tgaactcggc ttccgggcct gggatgttgg ccccgaactg cgccgggaat gggctgggtg
     8461 ttccgaggct gccccagggg tcatcgaagg agacgtcctc gaaatcgtag atggggccga
     8521 acggcgggtc cacgtgcatg tagaccagcg cgctcagaaa cggcgtatag tagatgcccg
     8581 cgccaccccg caaactggtt ttgccgtcat tcgtgagccg gtaggcgaag ccgacacgag
     8641 gaccgaagtt agccaagttg ctctcatagc ctctcgaggg acagcgcgcg tccggatccg
     8701 gcccgccata gacgagtcca acgggcgcgt tgggatagcg cgctgacttc gcgccgggca
     8761 cgaaacaggc gacgcgtccc ttctcttcca cgaacggaag cgagggatcc caacgcagtc
     8821 ccagattcag cgtcagccgc ggcgtggcgc gccagttgtc ctggatgtac aggcttaact
     8881 tcgttcccgt catgtctttg aactcgccgg cgccctgcgt gaacgctgac actctgccga
     8941 gcatgaaatc cgccagattg ttgccggaca actgatccgc gaaagtgaaa ttgccgcttt
     9001 gtagccagtt ggaggccatg gtgttgcgga tgcgcacaac ctcgccgccg aagtgccact
     9061 cgtgagcgcc ccggattttg gtgacgtctt cgcgaatcgt ccagtcgctg cggtcgttct
     9121 tccctggttg gctggtggtg acgctgaagt agccgtccac ggagaaatcc acctcgcgtt
     9181 cgggcggggc tgcgatcttc atgcccgctt ccgggaatcc gaaggtggag ttcggtttct
     9241 gtccgccggt ttgcgaattc cagccgaacc aggtgctaaa gagcagcgac ggcgtcgccg
     9301 tgaacgtgta gctcacggcc acgttctgca cgcgcacgcc cataacgttg ccggacgcaa
     9361 tcagcagatt ggtttcggag aagtcgccgg gccggttgaa gtcggtaaag aagtaacgtc
     9421 catccaactg gtgttttccc gcgttatagt tcaacttcgc catccactga tgctcgtcgg
     9481 agttggcagc gccgcccgtg aacgtgatct gctggccgga gccattcggc aacggaatgc
     9541 ggtccacgac atacctggcg acggggctga ggcgcgactc cgggatgatg ttaccgggga
     9601 acggctgctt ggtgaccgga tctattagct ggcgggaaat gctcgagaaa tccccccggc
     9661 gctgcgcttc ggtgggaacg aaggcgatgc gaccctcggg cgccgttctt attggtgtgc
     9721 cctggtacgt gccgaagaag aacagcctgt cccgcttgat gggcccgccg atgctgccgc
     9781 cgaactggtt ccgcttgagc gaatcctgtc gcggagcgaa aaaattccgt gcgttgagct
     9841 tgccattgcg gagaaattcg aacgcagtgc cgtggatctc atttgtcccg gacttggtca
     9901 cgatgttgac cactccaccg gcagcattgc cgtattgcgc gctcagattg tcggactgca
     9961 acgcgaattc ctgaatcgcg tcaggattgg gaaagggcag attcaggttc agataggtgt
    10021 cgttgtggcc ggcgccgtca agctgatagt tcacctggcc cggcccggta ccgttcacgt
    10081 tggcaagttg ctcgccgggg tacacgcccg catagcccaa gctgcgcgac tggtcgaggt
    10141 ccacagtacc tgccgacagg tacagcaggg attgggcgcg ccggccgttt agcggcaggt
    10201 cgataacgcg gcgttcgtcg atcaactgcc cgacggtggc ggttcccgtc gtgaccaact
    10261 cgacattcgc cgacacgacg actcgctcgg agacctggcc aacgcgcagc gctacgtttt
    10321 gcgtcgcgga ctggttcact gtcaggacga tgccttcctg aaggtatgtc gcaaagccgg
    10381 gtttctccac cgtaagccgg tagttgccca ccggcagcct tggaaagaca aacaggccgc
    10441 tcactccagt cacactggag gcctcgaatc ccgtttcggt gttgtgtatt gtgactttcg
    10501 cttcaggcac agcggcgccg gttgcgtcaa aaaccacgcc gtttagactg gcggtagtga
    10561 actgggcgaa ggccggaacc gaacacatgg ccaagatcag aaaccttttc gcgatcccca
    10621 taccagaccc tctggcacgg aattatacag gggatctgtt gtcatgtcac tgcaatattt
    10681 tcgtctgtca atatttttgt tttctcactc ggcctgtccc cattcctcga ctttttccgg
    10741 tgccgggttc agaatggggc gagcaagcat tcctatcgcg cacgcgcgtg ttcagcgcgg
    10801 ttgcgccaag gagtcttatg accggcatag gaagaagacg atttatggaa atcgcgggcg
    10861 cgggctcttt agccgtgccg ttgaccggca cgccgacgcc ggtccgcgtc ggcgtgatcg
    10921 ggattggacg gcgcggaacc ggcctgctcc ggattctact caacatccct ggggtgcagg
    10981 ttccggcggt atgcgatacc aatgcggaca acctggccgc cgcggccgcc agtgtcgaaa
    11041 aaaaaggtca aaaacctccg gaacaatatg gcggcggtcc ggaggcgttc cgtaaactgc
    11101 tggcacgcga cgacctgcag gcggtggtga tcgccacgcc gtgggaatgg catgctccca
    11161 tggccgtggc ggcgatgaag gcaggcaagt acgccggcgt ggaggtcccc gctgcggtaa
    11221 cgcttgagga atgctgggaa ttggtcagca cctccgagga gaccggcctg ccgtgcatgc
    11281 tgctggagaa cgattgcttc ggtgacggtg cgctgatggc gctgaatctg gtagagcagg
    11341 gcgtgctcgg cgagatcgtc cactgcgagg gaggatacca gcacgacatc cgcgcgggca
    11401 ttctccgggc cgggcagttg tcgtggcgcg cgcggcacgc gctgaagcgc aacggagacc
    11461 tctatcccac tcatcccgcc ggtccgatgg cgtggtggtc gagcattaac cgcggagatc
    11521 gcttcgcata ccttacctcc acagccagca aatcgcgcgg catcaaccac tacatcaaga
    11581 agtactatgg cgccgaccat ccgcacgcta atgcgaagtt cgcactgggg gacgtcgtca
    11641 cgactgtcct gaagagcgag aacggcgtca ccatggtgat ttcccacgac accagcctgc
    11701 cccgccccta ttcggattcc ggcgaaacca gcattccgct catggtcatc cgtcttcagg
    11761 gaaccgaggg aatcttttcc ggaagcctgg accgcatcta tatcgaaggg cgaaacgggc
    11821 cgaattcgaa gatgcacaag tggcaggaca tccagcccta ttaccagcgt tacagacacc
    11881 agttgtggaa gaacctcggc gcggaagctg cgggctacga acacggaggc gaggactacg
    11941 tgtgcctcag ccagttcgtg gaagccgtgc ggcggcgcaa gcccacgccg gtggacgtct
    12001 acgacgccgc atgttggagc gccatctcgc cgctcagcga gcaatccgta gctgcacgga
    12061 gcgcaccggt ggaatttccc gatttcacgc gcggcaagtg gaagacgccg cgccgggtcg
    12121 agttcctcct ttgattaggg aatgaaggtg cctacggggt gccgtgctgg gtcgatcccc
    12181 accggccgag tgcaggatcg acgggttccg aaggagctgg aacttggcat gccaagccgc
    12241 aggcgtcccc gagctgctgt cgagcgggtg tcgctgagtc ggtgattatg aatcgatcaa
    12301 aaacggtaaa ggccgctgta tcaatgcgat acagcggcct caaatatggt gcggagaggg
    12361 ggacttgaac ccccacgaga ttgctcccgc tagcacctca agctagtgcg tctgccaatt
    12421 ccgccacctc cgcagtacaa cctaattcta cttcttatcc gcaggcggtt gttgtgctgg
    12481 aggcgccgtc tgctgagctg gcggttgctg tgccggagcg gctggcgttc ctggcgccgg
    12541 tgtctgttgt tgtgttgccg gtgccgtccg ctctaatacg gaacccccgc ctcccgtcga
    12601 actgccgcgc gttgccatga tcgacaatga catggatgtc agcatgaaca ccgtagcagc
    12661 aatcgttgtg gccttggaca ggaccgttgc cgcaccccgc ggcccaaacg ccgtctgcga
    12721 gcccattcca ccgaaagcac ccgcgaggtc tgccgccttc ccgctttgca aaagcacaac
    12781 cacaatcaga aagaggcaca caatgacgtg caagattgta aacaaaatga ccatatcaag
    12841 ggctcaacag gctggatgtc tgttctttct cagtataaaa gaccgttcgg tcccggtcaa
    12901 acagccccgc gctctctttg ttggacccca tcgctccaga aaataaatat atcgtagtac
    12961 gatgtaaaag tgagtacttc ttcgacagac ctggctcccg gtgactacca ggcgttggcg
    13021 gaattccgct atcaaatccg gcgctttcta cagttcagcg agcatgcggc gcgcgagagt
    13081 ggcgtggaac cgcagcagca ccaggctctg ctggctatcc gcgggatgaa gccggaaaca
    13141 caacccacga tcggggaaat cgcagcgcgt ttgttcctgc gccatcacag caccgtggaa
    13201 ctggtgaacc gcctggaaca cggcggcctg gttgctcgcg aacggcattc cgaagacgga
    13261 cggcaggttc tggtgcgtct cactcccgca ggtgagcgtg tgcttcgcag actttccatc
    13321 gcacaccgct cggaattgca ggtgaccggc cccgaactcg cgcgagcctt gcagcggctg
    13381 acccggcgaa acaggaaatc cgaagcggca tgacaccaaa ggaggtctgc caatcggccg
    13441 aggggcacac ccggagcgtg gacgaactcg gcgactttac cgcgacaccg cgcatgatcc
    13501 caatttgctt actggcagtt gtgatcggcg ccggcagttc gttcgtcgcg ctagcactgc
    13561 taaagctgat cggcttattc acgaacctgt tctatttcca acggctaagt acggagctgg
    13621 tgccgccggc gggcaaccaa ctggggtgga tggctgcctt ggttccggta ggcggcgcgc
    13681 tcatcatcgg attgatggcc cgctacgggt cggagaagat caggggccat ggcattcctg
    13741 aggcgatcga gtccattctc atcaacggaa gccgtgtaca accgcgcctg gcgattttga
    13801 aacccctctc cgcggcggtt tcgatcggtt ccggcggtcc atttggcgcg gagggcccca
    13861 ttatcatgac cggcggcgcc ttcgggtcga tgtttgccca gttcttccgg ttgagtagcg
    13921 ccgagcgcaa gacgcttctg gttgccggag cgtcagccgg tatgtccgcc acattcgcgg
    13981 ctccattgtc ggcggtgttg ctggctgttg agctactcct ttttgagtgg aaaccccgga
    14041 gcatgattcc cgtcgccctg gcgagtgcga cagcaggagc cgcgcgccgg tatttgatcg
    14101 gcgtaggacc gctatttcct gcgcccgatc acccggtttt cgccggcata tgggtcctgg
    14161 tggcatgcgc catcgccggc gtgctggcgg gcttgctttc gacgatatta acggcggccg
    14221 tctatgcggc cgaagatgcg ttccagcggc tgcccatgca ttggatgtgg tggcccgcca
    14281 tcgggggcgc cgtagtggga ctcggcggac tggcctttcc tcaggcgctc ggcgtcggtt
    14341 acgagaccat cggggagctt gtgcagggtg atgtcgcgaa agggacgctg gcaggcatcc
    14401 tgctcgtcaa atcggtcatc tgggctgtgg cattgggctc cggcacctcc ggcggggtac
    14461 tggcgccgtt gctgatgatg ggcggggcgc tcggcggact cgaagcgatg tttcttcccg
    14521 atgcagggct gggattctgg ccgctgatca gcatgggagc gatcctcggc ggcacgatgc
    14581 gttccccatt taccggggtt gtttttgcca ttgagttgac acacgacgta aacgtactgc
    14641 tgccgttgct gatcgccagt gttgtggctt acgccgttac ggtacttctg ctcaagcgat
    14701 cgatactcac cgaaaaagtg agcagaaggg gctttcacct gagccgcgaa tactcggtgg
    14761 atcctctgga gatcctgttc gttcgcgagg tgatgcgaac caccgtggcg gcgtttcccg
    14821 cctccgccac ccttgatgaa cttgctgctt ctttccgtcc cgatcacacg ccccgagggc
    14881 aacatcttta tccggtcgta gatggcggca atcgcctgat cggagtgatt acccgtaagg
    14941 acatcgtgag ccttctggca cgccgcgcga gagggactgt agcggagttc gcccgcacag
    15001 accccattgt cgcctgcgcg caagagccgc tgcgagctgt tgtttttcga atggcggaga
    15061 ccgggttcac gcgcatgccc gtcgtggaca ggacgagtgg agaccggctg gccggcatgg
    15121 tgtcgctcga ggatctgttg cgagctcgag cccgcaacct caccgaggag cgccatcgtg
    15181 agcgtgtgct tcgcatcagg ctgccattca gcttagccgc caaacaggct tccaccggtg
    15241 cagactgaga ctcgcggacc ggcaccacta aggttgttcc gcggccatct ggaacggttc
    15301 gtcggcgctg ccaccgtaga tgtccggcac cttcgctccc atcggccgga gatacgccga
    15361 aagctgccct ctgtgatgca cttcgtggag gagcaggaaa ttcaggtaga tggcaagagg
    15421 aaatttcatg cctaagaagt cgacgatctc tgccatctgt ttagggg
//
LOCUS       DASTMK010000125        15602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_390040, whole genome shotgun sequence.
ACCESSION   DASTMK010000125 DASTMK010000000
VERSION     DASTMK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15602
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390040"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3019)
                     /locus_tag="VFQ79_05140"
     CDS             complement(<1..3019)
                     /locus_tag="VFQ79_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0139068.1"
                     /translation="MQRVMLIAALMTACAGIMLAQATSGVITGLIIDPSGAPVPNVTV
                     EIRNQGTNELRRVASNQVGMYEVPNLVPGLYTVKAQAQGFRSIENRDLHLEALRTLRV
                     DFKLEVGSVETAVTVIGGAPVVETETATIYSVRTIRQLQEMPLNLRTMQGLTGDTGAW
                     AYINVTPTGQGRGVTMSLGGGRASMSDVNVDGISTRSPAFGNLLPTAPAIDTVQEVRF
                     DYVNNRAEFSEMGNITLVTRGGSNEFHGRVLWDHGNAALNARNFFATARPAYIRNDFG
                     GTVSGPIVGDKVFFLANFEAQRVRQAATIAPSLPTLKMRGGDFSELLGLSSPIVLQNP
                     FTNSPYPGNIIPPDMLNQGALRYQDRFLPEPNFGPPDLFVSNFRQNYPQAHRQDHSTS
                     RIDYNISANHRLYGRITFQRSDFNPLESGLPPENVGYREQVRNNRNVVISETWTVSPT
                     VINEFKAGYSRDYNPRSGPIAGQTVTELLGMRGIVPQPESNKAYPSVGISGFTTLSQT
                     VPAYPAQNVFQASDQVTIIRGRHTFKTGIDFIPQQSNETTPAPFPSLSFNGNYTGFAY
                     ADFLIGLPNSTGRSFQSLPRTYGYYYLNGFVQDDFKVSQTLTVSFGLRYEYNSPVVDR
                     FDTVTNFSPAIGGYVIPNEATRAKLNPLYPKEVPIVLAQEAGYPERSLRNPDKNNFLP
                     RFGFAWRPFGGTKTVLRGGYGLFNDSLTSQHARLSGGPYSGSESFFNSITNGVPLLTL
                     QTPTLDQGTPTLRTGSLSASGMDVNLRNPYAHQWNLTIERDLGFETALRVSYIGTKAA
                     QLLYSRNINQLPPSTQPFDQSRVPYPNFTSISMLENGANQTFNALSIDVEKRWSRGLY
                     FQAAWTWSKSLTDVDEWAGVAETGLQIENAYDRSRERGDSQYTPRHKFIANVIWELPV
                     GSGRSFLNRTGLLNYVLGGWNLSSSYQGQGGHYLSPVFSGPDPSNTRTFGGLPDRIGD
                     GNLPKDQRTVDRWFDASAFTVPPNGRFGNSGRGI"
     gene            complement(3269..4435)
                     /locus_tag="VFQ79_05145"
     CDS             complement(3269..4435)
                     /locus_tag="VFQ79_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0139069.1"
                     /translation="MKDDRKTGAAGYRLETVSRACSLLRIFREGESLRLNEMVSRTGL
                     NKSIVFRIVRTLEEAGMLRNTGAHRYECNIRFPADRKFRMGYAAQADNSQFSLAVTES
                     LRRAAARELIDLIVLDNRYSARAALRNAERLISERVDLAFEFQTYERVAGVVSSRFQE
                     AGIPVIAIEIPHPGATFYGANNYRAGLTAGRALARWAKQNWAGAVERVLLLELRIAGA
                     LPQLRLEGAEAGIREILPGLSPLSFVHLDARGDFDCVLEATRKHLRRTADLHTLLVGV
                     NDPSALGALRAFEEAGRSPSCAAIALGATADARAELCRPGSRLIGSVAFFPELYGEDL
                     IRLALDILAKRHTPPAVYARHQLITSENLPQYYPHEFSARAVAGKTHPASAAAL"
     gene            4928..5725
                     /locus_tag="VFQ79_05150"
     CDS             4928..5725
                     /locus_tag="VFQ79_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0139070.1"
                     /translation="MDGLGFYEEAVDPPLAHAQSSSDVEHYPWPDCSEWDAGGLRAEC
                     LSWKGYPIVGASYEPLYLYCRLRGMEQALEDLVLGSPVVDAAMERIYEIHAGIVRRVL
                     DAAGDLIDFIYVAEDLGTQNSLLMSPRLFRRYIKPWLAKMIELAHSRGVKAFHHDDGA
                     IRGVLADLIEIGIDVLSPIQWRCRGMERKALVRDFGNAVAFHGGIDNQQTMPFGTPAD
                     VWNEVAANVSIFKKAKGYIVAPCHNLQANTPTANILALYEAVQELSA"
     gene            5722..7773
                     /locus_tag="VFQ79_05155"
     CDS             5722..7773
                     /locus_tag="VFQ79_05155"
                     /inference="COORDINATES: protein motif:HMM:NF019120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139071.1"
                     /translation="MKAGWRERLRRRGLALCLVAVSLPVLGAAPPAVVIGNGLAGDLS
                     LELAAAELRAALARTEAAGKRILLDVRGPLENRAVEHQAYVIEPDSRGLRITSSGAGG
                     IYGAYRLAEMIRSDGVSWDLRVSAEPAFAERMFSYEGTVFSLPDEGYYFRDPPYVNHI
                     VLNEQIEAAKEAMRKLARYGFNSVAFLHLNIEDYVNYDLLGDGTRVYSPDSLHRRRSE
                     VFIKALSDLAAYAHRLHMQLFIQVYEFSLPDHLDGRRIGDDSAASWEMIDAKYTELLR
                     KAPIDGIVVTATEPSPRLNYRGFILWKTQAGAGRMAARMQQVIVNKNHRRMIFRLWRV
                     ANDAAQFEQVLSTATDPDILFDAKHTDGDFFLNVGENRVLREGGSTGRAFMVTFDSFR
                     EFDGWGRTIFYPRFWGERFRGLKRNGVVAVNAWGPWVPGCIYPGVWVGKYDEYDFVRH
                     GHSPSLATLYLFSRLAWDPDLDPDVVAEDWVKLEFGPESKPIREALALSEELWLTTYI
                     DSHPYSQIAFKWTMLFLARPEIIERMGPGWSVSTIRDLNARGVALAKKMRQLVYSVTP
                     GRQFDPALEEMRRAAGLTLLFFETFTRWRELLYLRYRSPSGMAAEDRSAAHRLVAELE
                     GILPEWRQYPHEAKDWLIFRFDPDLITAPAWMTRTSVAETVAGLKKELADQSAGTGHK
                     P"
     gene            7770..10733
                     /locus_tag="VFQ79_05160"
     CDS             7770..10733
                     /locus_tag="VFQ79_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEU0139072.1"
                     /translation="MKFKPSRGIAPCILSLSFFALPRAIAFEGVPFHVADTLGIARTG
                     EVVTSGLPLAKGALRDGEAVHVSTAEGRALPTQSRVLGRWPDGTVKWLLVNFPVDSPA
                     NGNARYHLAHGSGSAQPTRLHIRDEASQFIVDTGAIRAVVAKNRMTLLSGVERGGMAI
                     LSEGAPLRIETADAGSYSSADAIPESVELEERGPVRATIRVRGWLAGSERRYRLDVRL
                     RFYAGQPYVCAEYTCIVLGGPKVHQVREMRVDLRLSSVAGDFVMPHGFAGTLTKPAIL
                     EVRPDRNAAFGFEGDVKPAQRPIEGWALLESALGVAVADFRHTGPKAIELNREGIKVA
                     LWSGRRGDVLRFGRGRARTHHLLLDFGGSQARLRAFEQPLIATPDPEYFCRTEAMGML
                     SPEGAPATAGYDRKLRRAFNELYRQRMETPRESGFLHYGDYYHGGYGNEVTRGSLEYD
                     TAHGAFMLWARSGRREYYDYALTSNRHFVDMDINHETGDQLFHGYTETAENHEEIKTR
                     TEYGHVFVDGPADAYYLTGDERSLEAVRTIAGKVAEISAGEDYSRIRAIMGRAERNLG
                     WPLQLLCRAYEVTGDERYLAAGRKIVEYVKIYARDPLAEYRQGNWWRSWMMDGSKAFM
                     IGALHDGLEAHWQVHPDPELRQVFTKSLDWMIDHMWRPTGEFVYEFNAMNRPHRTQLS
                     SLNFLFIDAFRFGYTLTGDRRYLAVALAGFNARVTEWEFPLDGKQFSIDIRKSPHTAA
                     FFYREKLLHDKLPPAPQPVFQTAKPDPRTPRPDLLLRASFEGNLEVDAPAGKVQGGLV
                     GKADFTDGKRGQGIAVGKNGYAWLPAPPEMLMAPGTIELWVRLNFKKQPLEPEQSAVF
                     HVEGKTPLADTLGAMTIYHEMRVRMKDSAGHLNGTAEGEITQWNPGEWHHLAVTWDAT
                     RIRLYLDGKEQIRPDEGKYLWDGVALLPAGGQTRINLGWRFGNWYCDSTIDELAIHGR
                     AFRPDEIRARFESFQ"
     gene            10730..13369
                     /locus_tag="VFQ79_05165"
     CDS             10730..13369
                     /locus_tag="VFQ79_05165"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU0139073.1"
                     /translation="MMRIALLILLAFAPPAFSNDWESGFADPGPEHAVGVYWWWFGPA
                     QTKDEVDRELRVMKEARIGRVLIFPIYPIAADGPARGIRNYPYLSAEFLDVLRQTVQT
                     AQRLGIEVDLLLSTGWPYGGSWIPESLSARRLRVEGKSAPQILPEHSVIARIGPMVFF
                     DAPTGMRVKRPALGAEGFVMDHLSREALSHHLTEVGDKLLDAAPGIRAIHSDSMEVYG
                     QNWTKNFASEFKKRRSYALEPHLPALFDKEHPQAPDIRHDYWLTISDLVVDHYIKPLA
                     QWTRSRGVGLQAESYGTPAVDMRTFAHVDHPMGESYDWKTFVASRWASSAAHQFGKRI
                     TAAEALTWLRQPRYISTLEDMKVGADLHFACGINKIIAHGYAYSPPSAGVPGWGYYAS
                     VMLNDRNTLWPFFPLLSNYIRRASYALSLGKPRVDIGVYLPEDDVRAAQGLDSGPDLY
                     KTSLNLYMATKYHLGTGAVPEFGLPAAYASETPLIKTILSAGFGFDGFDHAILRPELH
                     TEGGRLAIGDVAYRIAVLPNVKGISTAMLERLADFCRAGGVLIATRRLPDTAYGLKDR
                     ERSVARVRELVAELFGKQQSGETRRRTYGKGLAIFVPDETKELAAVLSSLRPQIRLGH
                     PDSDLVFIQRGAEERELYFLANTSDRKKTFRALFRDGKSRPRMWDAMTGQAWDLPDDG
                     NSVALTLEPYASIFVAFEGLVETTAAKSAPAAFAERASIAAPGPWLLDIEGRQVKLDD
                     LKSWTAIPDYHYYSGRGDYRTEIRIPANWLNPGDSVWLDLGSVKEVADISINGKPAGV
                     CWMRPYRLEVSDLVRPGMNQIKIGVTNLLINAVLGQPTPDFSKLKVIQFPLPTEKKMI
                     KEPLPSGLLGPVRVVQGGSRNLQ"
     gene            complement(13370..14269)
                     /locus_tag="VFQ79_05170"
     CDS             complement(13370..14269)
                     /locus_tag="VFQ79_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0139074.1"
                     /translation="MRQRFFFAIWFAWLAAFTLQAAPESPIPLWPGTAPGDTGGIGPE
                     HDTTAAKDALVAGGRVTRITNVTTPTLTLFRAPDDKRTGAAVLVFPGGGYRILAWDLE
                     GTEICEWLNKIGITAVLVKYRVPAREGLPRYAAPLQDAQRAMGIVRARAKEWDIDPGR
                     IGVLGFSAGAHLSAALSNNFEKRTYPRVDANDDINCRPDFAVLIYPGLIVPRNSSSMD
                     IEFQVTSATPPAFLVQTADDGVRVENSLAYYSALKAAKVPVEMHLYPSGGHGYGLRPS
                     PNLVSTWPARAEEWMRSLGVLRR"
     gene            complement(14285..15502)
                     /locus_tag="VFQ79_05175"
     CDS             complement(14285..15502)
                     /locus_tag="VFQ79_05175"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139075.1"
                     /translation="MSTMQSSNTKPVRLGFVGLGARGTYHLKAALMVQGVEVAALCDV
                     DTARLEQAAGLVRGAGKAASKRYGKGEKDYERMCGEETLDAVICSTPPQSHAAVCLAA
                     NRNGKHAASEGPLALTLENAWALIEAYEKTKKWSALALETTLLEEENGNNLTLLNLVR
                     NGLLGDMIHCEDGVLRDRRAAKLREMQRSSTALGDSGNFAPDAPMNRLIPLMDINHGD
                     RFDSLMSVSSRPAAMAEFASTKLGKEAAAKFRNGPGDYNASILHTVDGKLVTLNYDTS
                     TPNPREYCRFQGTKGVYMSAPGLPGPMIYIDGISVRPHRWEKVDSYFEQHRHPLLGKA
                     AGNKTPLTWQLLVAALREGKQPYFDVYDSVTSSAVIALTQKSVSSRSRPVEFPDFTRG
                     QWKTRKPIDLLTV"
BASE COUNT         3189 a         4456 c         4683 g         3274 t
ORIGIN      
        1 tgatgccccg tcctgaattg ccaaagcggc cgttcggagg gacggtaaat gcgctggcat
       61 cgaaccaccg gtctaccgta cgctggtctt tcggaagatt tccatcgccg atgcggtccg
      121 ggagacctcc gaatgtccgg gtgttcgaag gatccggtcc actgaataca ggcgacagat
      181 agtgaccacc ctgaccctga tacgaactgc tgaggttcca tcccccgaga acatagttca
      241 gcaggccggt ccgattcaga aagctcctgc cgctgcccac cggcagttcc cagattacat
      301 tcgcaatgaa cttatggcga ggcgtgtact gggaatcgcc gcgctcccgc gaccggtcgt
      361 aggcgttttc gatttgcaga ccggtttccg ctacgcccgc ccattcgtcc acatcggtca
      421 ggctcttgct ccaggtccag gcagcctgaa agtataggcc tcggctccaa cgcttctcga
      481 catcgatcga gagagcgttg aaagtctggt tagcgccatt ctcgagcatc gatatgctgg
      541 tgaagtttgg atatggcaca cgagactggt cgaagggctg tgtgcttgga ggcaattggt
      601 tgatatttcg ggaatagaga agttgcgcgg ccttggttcc gatgtaggag acacgcaacg
      661 cagtttcaaa tcccagatcc cgctcgattg taaggttcca ctggtgagcg tacggattgc
      721 gaaggttgac atccattccg gaagcgctca agctgcccgt gcggagggta ggggttccct
      781 gatcgagcgt aggcgtttgc agcgtcaaca aaggaacacc attggtgatg gaattgaaaa
      841 aactctcgga gcccgagtac ggcccgccgc tcagccgtgc gtgctgtgac gtgagcgagt
      901 cgttaaaaag cccgtatcct ccccgtagaa ccgtcttcgt tcctccaaaa ggacgccagg
      961 cgaatccgaa acgcggaagg aagttgttct tatccgggtt gcgaagcgac cgctccggat
     1021 agccggcttc ctgcgcgagc acgatcggca cttcctttgg gtataaaggg ttcagcttgg
     1081 ccctggttgc ctcgttggga atcacgtaac ccccaatcgc cggactgaaa ttcgttacgg
     1141 tgtcgaatcg atcgaccacc gggctgttgt attcatagcg caatccgaag ctgaccgtca
     1201 gagtctgcga gactttgaaa tcgtcctgga caaagccgtt caggtaatag tagccgtaag
     1261 tgcgcgggag tgattgaaaa cttcgtcccg tgctgttcgg gagcccgatc agaaaatcgg
     1321 cataggcgaa accggtgtag ttcccgttga aactcaagct cgggaaaggc gccggcgtgg
     1381 tttcattgga ttgctgcggt atgaaatcga taccggtctt gaaggtgtgc cggccgcgaa
     1441 tgatcgtcac ctggtcggag gcctggaata cattctgtgc cggatatgca ggtacggtct
     1501 ggctgagtgt ggtaaagccg ctgattccga cggacggata cgctttgttc gattccggct
     1561 ggggaacaat cccacgcata cccaacagct ccgttaccgt ttgtccagcg ataggaccgc
     1621 ttcgcggatt gtaatccctt gagtaaccgg ctttgaactc attgatgaca gtcgggctga
     1681 cggtccacgt ctccgagatt acgacgttgc ggttattgcg cacctgttca cggtagccga
     1741 cgttttcggg aggcaaaccg ctttccagtg gattgaaatc agagcgctga aacgttatgc
     1801 ggccgtacag gcgatggttg gcgctgatgt tgtaatcgat tcgcgaagtg ctgtgatcct
     1861 gccggtgtgc ctgcggatag ttctgcctga aattcgaaac gaaaagatcc ggtggaccga
     1921 agttcggttc cggcagaaaa cgatcctgat accgtagagc cccctgattc agcatgtccg
     1981 ggggaatgat gttgccagga tacggactgt tcgtaaacgg gttctggagc acaataggac
     2041 tggacaagcc cagcaactca ctaaagtccc cgccacgcat cttcagagta ggcagactcg
     2101 gagcaatcgt ggcagcctgg cgcacgcgtt gtgcctcaaa atttgcaagg aaaaacacct
     2161 tatcgccgac tatggggccg ctcactgttc ctccgaaatc atttctgatg tatgcgggtc
     2221 tggccgtagc gaagaaattc ctcgcattca gtgctgcatt gccatggtcc cagagcactc
     2281 gtccgtggaa ctcgttgctg ccgcctcgcg tgaccaaagt gatgttcccc atctcggaaa
     2341 actccgccct gttgttcacg taatcaaagc gcacttcctg tacggtgtcg atcgctggtg
     2401 ctgtcggcaa aaggttgccg aaggccggcg agcgtgtgct aatgccgtca acattcacgt
     2461 cgctcatgct tgcgcggccg cccccaagag acatcgtgac gccacggccc tgtcccgtcg
     2521 gagtcacatt gatataagcc caagcgccgg tgtcgcctgt gaggccctgc atcgtccgaa
     2581 gattgagggg catctcctgg agttgcctta tggttcggac ggaatagatg gtggcagtct
     2641 ccgtttctac cacaggagcc ccgccaatca ccgtcaccgc cgtctccacg ctgccaacct
     2701 ccagcttgaa gtcgacccgc agcgtgcgga gagcttcgag gtgcagatcg cgattttcga
     2761 tactcctgaa accttgcgcc tgggccttca ccgtatagag gccgggcaca aggttaggca
     2821 cttcgtacat ccccacctgg ttgctggcga ctcttcgaag ctcattggtg ccctggttgc
     2881 ggatctcgac ggtgacgttc gggacgggag caccgcttgg atcgatgatc aaacccgtaa
     2941 tcacgccgga agttgcctgc gcgagcatga tgccggcgca cgctgtcatt aacgcagcaa
     3001 tcagcatgac cctctgcatc ggaagcctcc tgaagactat cggtgacctg gcacacgtgg
     3061 cgcctcacca gagtggagta tcggcgctga tgccgccagg gtcaacactt atattggagg
     3121 gagcaattcg tttcggaaaa cgaatcgagc gttcgtccag ttttgagtcc gaaaggtttc
     3181 tttatcagtg cgtaaggaca aggacgcttc cacttccaaa ctccggcatc ggccgcgcat
     3241 agtttctgtt tgcgcaacgc tgctgaagtt acagggcggc agcgcttgcc ggatgtgttt
     3301 tgccggccac tgcacgtgcg ctgaactcat gaggatagta ctgcggcaag ttttccgacg
     3361 taatgagttg atgccttgca tagacagcgg gtggtgtgtg gcgcttcgcc agaatgtcca
     3421 gcgcaagccg aatcaggtcc tcgccatata attcggggaa gaaagcaacg gacccgatca
     3481 gccgggaccc tggcctgcac aactccgccc tggcatcggc tgtggcgccc agagcgattg
     3541 ccgcacagga gggagaacgt cctgcttcct cgaacgcccg cagcgctccg agagcgctgg
     3601 ggtcgtttac accaacgagc agcgtgtgca ggtccgccgt gcggcgtaag tgttttcgtg
     3661 tggcttccag gacgcaatcg aaatctccgc gagcatcgag atgaacgaag ctcagagggc
     3721 ttaggccggg aagtatctcc cgaatgcccg cttcggcgcc ctccagccgc agttgcggta
     3781 acgcaccggc gatgcgcagt tcgagcagaa gcacgcgctc cacagcgccg gcccaattct
     3841 gcttggccca ccgggccagc gcccgtccgg cggtgaggcc ggcccgataa ttgttggcgc
     3901 cgtagaaggt ggcccccggg tgaggtattt cgatcgcaat tacggggatt ccggcttcct
     3961 ggaaccgcga cgagaccacg cccgccaccc tttcgtaggt ttgaaattcg aaagcgagat
     4021 ccacgcgctc cgagatcagc cgctcggcgt tccgtaaagc agctcgagcg ctatagcggt
     4081 tgtcgagcac gatcaagtcg attagctctc tggccgcggc gcgccggagg ctctcagtta
     4141 ccgcgaggga gaactgcgaa ttgtctgcct gagcggcgta gcccatccgg aacttccggt
     4201 ctgccgggaa gcggatattg cattcatagc ggtgggctcc ggtgttgcgg agcattccag
     4261 cctcttcgag ggtacgcaca attcggaata cgatggactt gtttagcccg gtgcggctta
     4321 ccatctcgtt caggcggaga gattctccct cgcgaaatat acgcaaaaga gagcacgccc
     4381 gggagacggt ttccaaacgg tagccggcag cgcctgtctt cctatcgtcc ttcattgcct
     4441 ctggaacaac cccagtcggg gacggttcgg atatagaaat tttgctcgtc gatgacaacc
     4501 gcccctacca gcactactgg cgacaatata tcacacgcta gcgatgataa tgttgacgcc
     4561 caggccccaa ccggacatct tcgaggcaat tcgatatccg gcacgatgcc gccgcccgga
     4621 agcccatgat tgacgggatg cggagcgcag accatacttc gtgatgatga ccgccagaga
     4681 gcgatggctg tccaccatcg agggcaggtg ccccgatcgt gtgccgtgcg actattgggg
     4741 gacttctgag gtgacgcgtc gtttgatgcg gcatctgagc tgcgattcgg aacgcgcatt
     4801 gtgggaatgt ttgcgaatcg ataaatgcat cttcctcgcc ccgaaacacc caagcgcgaa
     4861 ggagaacaca tggcacattc catcgcagtt tagcgtttgg ggaattccca cagcgatggt
     4921 tccctatatg gacggcctcg gattttatga ggaggcggtg gatcccccgc tagcgcacgc
     4981 tcaatcctca tcggacgtgg agcattatcc ttggcccgat tgcagcgaat gggacgccgg
     5041 cgggcttcgg gccgagtgct tgagctggaa agggtacccc atcgtcggag catcttacga
     5101 gccgctttac ctctactgcc ggctgcgtgg catggagcaa gctctcgaag acctggttct
     5161 aggttctcca gtggtggatg cggccatgga acggatctat gaaatccacg ccggcatcgt
     5221 tcggcgggtg ctggatgccg ccggcgacct catcgacttc atttatgtgg ctgaggacct
     5281 tggtacacaa aactccctgc tcatgagtcc tcgtttgttc cgccgctaca taaagccatg
     5341 gctggccaag atgatcgaac tggcacattc gcgcggagtt aaggcgttcc accacgatga
     5401 tggcgccatc cgcggcgtgc tggccgattt aatcgagata ggcatcgacg tgctgagccc
     5461 gattcagtgg cgctgccgcg gcatggagag gaaagcgctg gtacgcgatt tcggcaatgc
     5521 ggtagcattc catggcggta ttgataatca gcaaactatg ccgttcggga cgccggcgga
     5581 tgtttggaac gaggtcgcgg ccaatgtgtc aattttcaaa aaagcgaaag gctacatcgt
     5641 cgctccatgc cataacctac aggcgaacac tccaacggcg aacatcctgg cgttgtacga
     5701 agcggtgcag gagttgagcg catgaaagcg ggatggaggg agcggctgag gcggcgcggt
     5761 cttgcgttgt gcctggtagc agtgtcgctc cccgtcctcg gcgctgcgcc tcccgccgtc
     5821 gtcattggca acgggttggc cggtgacctc tcgctcgaac ttgccgcggc agagttgcgt
     5881 gccgcgcttg cccggaccga ggcggcggga aaacgcattc tgctcgatgt tcgcggcccc
     5941 ctcgaaaacc gtgcggtgga gcatcaagct tatgtcatcg agcccgacag ccggggactg
     6001 cgaatcactt cctccggcgc tggaggaatt tacggtgcct accgccttgc cgaaatgatc
     6061 cggagcgatg gcgtgtcatg ggacctgcgt gtgtcggcag agccggcctt cgccgagagg
     6121 atgttttcct atgaaggaac agtcttcagc cttccggatg aaggctacta tttcagagac
     6181 ccgccttatg tgaatcacat cgtcctgaac gagcagatag aagcggcgaa ggaagcgatg
     6241 cgcaagcttg ctcgctatgg cttcaacagc gttgcgtttc ttcatttgaa tatcgaagac
     6301 tatgtcaact acgacttgct tggcgacgga actcgggtct atagccccga cagcctgcac
     6361 cgccggcgct ctgaggtatt tatcaaggcg ctttcggatc tcgccgccta cgcccatcgt
     6421 ctgcatatgc agttgttcat acaggtgtac gagttctctc tcccggatca tctggacggc
     6481 cgccggatcg gcgacgattc cgccgcaagc tgggagatga tcgatgcgaa gtacacggag
     6541 cttttgcgga aagctcccat tgacgggatc gtcgtgactg ctaccgagcc atccccccgt
     6601 ctgaattatc gtgggttcat tctctggaag acgcaggcgg gagctggacg catggccgcc
     6661 aggatgcagc aggtaatcgt gaataagaac caccggcgaa tgattttccg tctgtggcgt
     6721 gtggccaatg atgccgcaca gtttgaacag gtgctctcaa cggccactga tcccgatatc
     6781 ctgttcgacg ccaagcatac cgacggcgat ttctttctga acgtcggaga gaaccgcgtc
     6841 ctacgcgagg gcggctcaac cggccgcgcg ttcatggtga cgttcgacag ttttcgcgag
     6901 tttgacggat ggggccgcac cattttctat ccgcgcttct ggggtgagcg attccgtggt
     6961 ttgaagcgca atggggtggt tgcagtaaac gcctggggac cttgggtgcc gggatgcatc
     7021 tatccgggag tgtgggtagg caaatacgac gagtatgatt tcgtgcggca cggccactcc
     7081 ccttccctcg ctacccttta tcttttttcc cggctcgcct gggacccgga ccttgacccc
     7141 gatgtggttg cggaagattg ggtgaagctc gagttcggtc cggagagcaa gccgatacgc
     7201 gaggcgcttg cgctgtcaga ggagctgtgg ctcaccactt atatcgattc tcatccctac
     7261 agccagatcg ccttcaagtg gacgatgctg tttttggcgc ggccagagat catcgaacgg
     7321 atgggtccgg gatggagcgt gtccactata cgggatctca atgcacgcgg cgtggcgctc
     7381 gcaaaaaaga tgcggcaact ggtgtactcg gttaccccgg ggaggcaatt cgacccggct
     7441 ctggaggaga tgcggcgcgc ggccggcctg actcttcttt ttttcgagac gttcacacgc
     7501 tggagggaat tgctatacct gcgatacaga tcaccgtccg gtatggcggc agaggatcgt
     7561 tccgccgcgc accggctggt ggccgagttg gaaggaattc tgccggagtg gcgccaatac
     7621 ccgcacgagg cgaaagactg gctgattttc cgcttcgatc cggatctgat caccgcgccc
     7681 gcgtggatga ctcgtacaag cgtcgccgaa acagtggcgg gtctcaaaaa ggagctggcc
     7741 gatcagagcg ccggcacggg gcataagcca tgaaattcaa accttcccgc gggattgcac
     7801 cctgtatact ctccctttcg ttcttcgccc tgccgcgcgc aatcgcattt gaaggagtgc
     7861 cgttccacgt cgctgacact ttgggtattg ccagaaccgg tgaagtcgtc acttccggcc
     7921 tccccttggc gaaaggagca ttgcgggatg gggaggcggt gcacgtctcc actgcggaag
     7981 ggcgcgcact ccctacgcag agcagagtct tgggccgttg gcccgacggc actgttaagt
     8041 ggctgctggt caactttccg gtggattccc cggcgaacgg caacgcgcgg taccacctcg
     8101 cgcacggttc gggttccgca caaccgaccc gtcttcatat tcgcgatgaa gcctctcaat
     8161 tcatcgtcga tactggcgcg atacgagccg ttgtcgccaa gaaccgcatg actctgctaa
     8221 gcggcgtcga acgcggcggg atggcaatat tgagcgaagg agcgccgctc cgtatcgaga
     8281 cggcggatgc gggttcgtat tcgtccgcgg acgctatacc tgaatccgtg gaattagaag
     8341 agcgtggtcc tgtccgtgcg acgattcggg tccggggatg gctggctggt tccgaacgcc
     8401 gctacagact ggatgtacgc ctgcgtttct acgcggggca gccctatgtc tgcgctgagt
     8461 atacgtgcat cgtgctcgga ggaccgaagg tgcaccaggt gcgcgagatg agggtggacc
     8521 ttcggctctc gagtgtagcg ggcgacttcg tgatgccgca cggcttcgcg ggaacgctca
     8581 caaaaccggc aattctcgag gtacgcccgg atcggaacgc cgcattcggc tttgagggag
     8641 atgtcaaacc agcacagcgc ccgatcgaag ggtgggctct gcttgaaagt gcgctgggtg
     8701 tagccgtggc ggattttcgc cacaccggtc cgaaggcgat cgaactcaac cgggaaggca
     8761 ttaaggtggc gctttggagc gggcgccgcg gcgacgtact gcgttttggc cgcgggcgcg
     8821 cccgcacgca tcatctgttg ttggattttg gcggcagcca ggcgcggctt cgcgctttcg
     8881 aacagccgtt aatagcgact ccggatcccg agtatttctg ccgcacggaa gccatgggta
     8941 tgctttctcc ggaaggtgcg ccggcaaccg ccgggtacga tcgtaagctg cgccgcgcgt
     9001 tcaacgagtt gtaccggcag agaatggaaa ctccgcgtga gagcggattc ctgcattatg
     9061 gcgactatta tcacggcggc tacggcaacg aggtgacacg cggcagcctc gaatacgaca
     9121 cggcacatgg cgcattcatg ttgtgggcgc gcagcggacg gcgcgagtac tacgactacg
     9181 ctctcacttc gaaccggcat ttcgtggata tggacattaa ccacgagacc ggcgaccaac
     9241 tgttccacgg ctatacggag accgcggaga accacgagga gatcaagacc cggacggagt
     9301 acggacatgt attcgtcgac ggccccgccg atgcatacta cctgacaggt gacgaacgaa
     9361 gcctggaggc cgtgcgcacg atcgccggca aagttgcgga gatttcggcc ggtgaagatt
     9421 acagccggat ccgggcgatc atgggacgcg cggaacgcaa cctgggatgg ccgctgcaac
     9481 tgctctgccg cgcctatgaa gtaaccggag acgagcgcta tctcgcggcg ggacgcaaga
     9541 tcgtcgaata cgtcaagatc tatgcccgcg atccgctggc cgagtaccgc cagggaaact
     9601 ggtggcggtc ttggatgatg gatggaagca aagcgtttat gatcggggcg ttgcacgacg
     9661 gcctggaagc ccactggcag gtacaccccg accccgagtt gcgccaggta tttaccaaaa
     9721 gcctcgactg gatgattgac cacatgtggc ggccaacagg tgagttcgtc tacgagttca
     9781 acgccatgaa ccggccgcac cggacccaac tctccagcct gaattttctt tttatcgatg
     9841 cgttccggtt cggctacacg ctgacagggg accgccgtta tctggcagtg gcgctcgcgg
     9901 gattcaacgc gcgtgtgacg gaatgggagt ttccgctcga tggcaagcag ttttcgatcg
     9961 acatccgcaa atcgcctcac acggcggcgt tcttttatcg cgaaaagctt ttgcacgata
    10021 aactaccacc tgccccgcag cctgtgtttc agacggccaa acccgatccc cggacgccgc
    10081 ggccggacct gcttctgcga gcttcttttg agggcaacct ggaagtggat gcaccggcgg
    10141 gcaaagtaca aggcggcctt gtcgggaagg cggatttcac cgatggcaag cgcggacaag
    10201 gcattgccgt aggcaagaac ggatacgcct ggctccccgc tccgccggaa atgctgatgg
    10261 cgccgggcac gatcgagttg tgggtgcggc tgaacttcaa gaagcagccg ctcgaaccgg
    10321 agcagtccgc ggtctttcac gtggagggca aaacgcccct tgcggatacg ctgggcgcaa
    10381 tgacgatcta tcatgaaatg cgcgtgcgca tgaaagacag cgcagggcac ctgaacggta
    10441 ccgccgaagg cgaaattact caatggaacc cgggtgagtg gcaccatctg gcggtcacct
    10501 gggatgccac ccgcatccgc ctgtatctgg atggcaaaga gcagatcagg ccagacgaag
    10561 gcaagtatct ttgggatggt gtcgccttgc taccggctgg tggtcagaca agaattaacc
    10621 ttggctggag attcggcaac tggtactgcg attcgacgat cgatgaactg gctatccacg
    10681 gacgcgcgtt tcggccggac gagatccgcg cacggtttga aagcttccaa tgatgcggat
    10741 cgcgcttctg attttgctgg ccttcgcgcc gcccgccttc tcgaacgact gggaaagcgg
    10801 cttcgccgat cccgggcccg agcacgctgt gggtgtctac tggtggtggt tcggacctgc
    10861 ccaaacgaag gacgaggtgg accgtgaact gcgcgtgatg aaggaagcgc gcattggccg
    10921 tgtccttatt ttcccgattt acccgattgc ggcagatggc ccggcacgtg gcatccggaa
    10981 ctacccttat ctgtcggcgg agttccttga cgtattgcgc caaacggtgc agacggcaca
    11041 acggctcggg attgaagtgg atcttcttct cagcaccggg tggccatacg gcgggtcctg
    11101 gattccggaa tcactgtcgg cacggcgatt gcgtgttgag ggaaagtccg ccccgcaaat
    11161 actgccggaa cattcggtga ttgccagaat cggcccaatg gtcttttttg acgcgccgac
    11221 cggcatgcgg gtgaagcggc ctgcgcttgg agcggaggga ttcgttatgg atcacctcag
    11281 ccgtgaggcc ctgtcgcacc atctcaccga agtaggagac aagctgctgg atgctgcgcc
    11341 gggtattcgg gcgattcact ccgacagcat ggaggtgtat gggcagaatt ggacgaagaa
    11401 tttcgcctcg gaattcaaga aacgccggag ttacgcactc gaacctcact tgcctgcgct
    11461 gttcgataag gagcatccgc aggcgcctga catccgtcac gattattggc tgacgatcag
    11521 cgacctggtc gttgatcatt acatcaagcc gctcgctcag tggacccgaa gccgtggcgt
    11581 gggcctgcaa gcggaatctt atggaacacc cgcggttgac atgagaacct tcgcccatgt
    11641 cgatcatccg atgggtgaga gctacgattg gaaaacattt gtggcatcgc gctgggccag
    11701 ttcagccgca caccagttcg gcaaacgcat cactgccgcc gaggcactga cgtggctgcg
    11761 gcaaccgcgc tacatcagca cgctggaaga tatgaaagtc ggagccgacc ttcacttcgc
    11821 ttgtggaatc aacaaaatca tcgcgcatgg ttacgcatat tcgccacctt cggccggagt
    11881 accgggatgg ggctactatg cttccgtcat gctaaatgac cgcaatactc tctggccgtt
    11941 tttccccctt ctttccaact acattcgccg cgccagctat gcgctgagtc tcggaaagcc
    12001 gcgcgtggat atcggtgtct atcttccaga ggatgacgtg cgggcggcac aggggctcga
    12061 ttcaggaccc gatctctata agacatccct aaacctttat atggcgacga agtatcatct
    12121 cggcacgggc gccgtcccgg agttcggact gccggccgcg tatgcatcgg aaaccccact
    12181 gataaaaaca atactctcgg ccggatttgg gttcgacggc ttcgaccatg ccatcctccg
    12241 gcccgaattg cacacggagg gcggccggct ggcaatcgga gatgtcgcct atcgtatagc
    12301 cgttttgccc aacgtgaagg gcatctcaac ggccatgctg gagcggctgg cagatttctg
    12361 ccgcgccggc ggagtgctga ttgcgacacg ccgcctgccc gatacagcgt acgggctgaa
    12421 agatcgtgag cgttcggtgg cgcgagtccg cgaactcgta gccgagttgt ttggaaagca
    12481 gcaatccggt gagacccggc gccggactta tggcaaggga ctggcgattt ttgttcccga
    12541 tgaaacgaaa gagttggcgg cagttctatc ctcacttcga ccgcaaatcc gcctggggca
    12601 ccccgattcg gatctggtat ttatccaacg cggcgcggag gaacgtgagt tgtactttct
    12661 ggcaaatacg tcggatcgga agaagacgtt ccgcgcgttg tttcgcgacg gaaagagccg
    12721 gcccagaatg tgggatgcca tgacgggaca agcctgggac ctgccagacg atgggaacag
    12781 cgtcgctttg acgctggaac cttatgcctc aatctttgtc gcttttgaag gactagtaga
    12841 aacaacagcc gcgaagtccg cgccggccgc tttcgccgag cgcgcgtcca ttgccgcgcc
    12901 tggaccatgg ctgctcgaca tcgaaggccg gcaagtgaaa cttgatgacc tgaagtcatg
    12961 gacggcgatc ccggattatc actactattc cggacggggg gattatcgga ctgagattcg
    13021 cattcccgca aattggttga atccaggcga ctctgtttgg ctcgatcttg gatcggtgaa
    13081 agaagtagca gacatcagca tcaacggcaa acccgccggt gtgtgctgga tgcgccccta
    13141 tcggctcgaa gtcagcgatc tggtccggcc tggtatgaac cagattaaaa ttggagttac
    13201 taacctgctc ataaacgccg tgctcgggca acctacgccc gacttctcca agctcaaggt
    13261 gatacagttt ccgctgccca ccgagaaaaa gatgatcaaa gagcctcttc cttcggggtt
    13321 attggggcca gtcagggtcg tacagggcgg ctcgcgcaat ctccaatagc taacgccgga
    13381 ggacgccaag cgagcgcatc cactcctcgg cacgcgcggg ccaggtgctt accaggtttg
    13441 gcgaaggtcg caatccgtaa ccgtgtccgc ccgatggata caggtgcatc tccaccggta
    13501 ccttcgctgc cttcaatgcc gaataataag cgagactgtt ctcaactcgt acgccgtcgt
    13561 cggcagtctg aaccaggaaa gcgggtgggg tagcagacgt tacctggaac tcgatgtcca
    13621 tcgaactcga attccgggga acaatcagac ccggataaat cagcaccgcg aaatcgggac
    13681 ggcaattgat atcgtcattc gcatctacgc ggggataggt gcgcttctcg aagttgttgc
    13741 tgagcgccgc cgacaggtgc gcacccgccg agaaaccgag cacgccaata cggccaggat
    13801 cgatgtccca ctctttcgca cgtgcgcgaa cgatgcccat tgcgcgttgt gcgtcctgga
    13861 gaggagcggc gtaacgcggc agtccctcgc gcgccggtac gcgatatttc acaagaaccg
    13921 ccgtgattcc gatcttattc agccattcgc agatttccgt gccttcgaga tcccaggcca
    13981 gaatgcgata gccgccgccg ggaaaaacga gcaccgcagc cccggtgcgt ttatcgtcag
    14041 gcgcccggaa cagagtgagt gtaggggttg tgacgttggt gattcgagtg acacgcccgc
    14101 cggcaacaag ggcgtccttg gcagccgttg tatcgtgctc gggtccgatc ccgccggtat
    14161 ctcccggagc cgttcccggc caaagcggaa tcggactttc gggcgctgcc tgcaaagtaa
    14221 aggcggcgag ccaggcaaac cagatggcga agaagaagcg ttgacgcata gtctatgaac
    14281 cccgtcacac tgtgagcagg tcgattggct tacgcgtctt ccactgtccg cgagtaaagt
    14341 cgggaaactc cacaggtctg ctccggctgc ttactgattt ctgagtaaga gctattaccg
    14401 cactcgatgt tacagaatca tacacatcaa agtacggctg cttaccttcg cgaagtgccg
    14461 caaccagtaa ctgccaggta agtggagtct tgtttcctgc ggccttcccg agcagtgggt
    14521 gccggtgttg ttcaaaatac gaatccactt tttcccaccg gtgcggcctg accgaaattc
    14581 catcgatata gatcattgga ccgggtaaac ccggcgcaga catgtacaca cccttggttc
    14641 cctgaaaacg gcagtactcc ctgggattcg gcgtactggt gtcgtaattc agcgtcacca
    14701 gcttcccgtc gaccgtgtgg agaatggacg cgttgtaatc tcccggccca ttgcgaaatt
    14761 tggctgcagc ttcctttcca agctttgtac tcgcaaactc cgccatcgcc gccggacggg
    14821 aactgaccga catcagagaa tcgaaacggt cgccatggtt aatgtccatc agggggatga
    14881 ggcggttcat gggagcatcc ggggcgaaat tgccgctgtc gccgagtgcc gtagaggagc
    14941 gctgcatctc ccgcagcttc gctgcacggc ggtcgcgcag cacgccatct tcgcagtgga
    15001 tcatgtcgcc gagcaaccca ttgcggacca gattgaggag cgtgagatta ttgccattct
    15061 cctcctcgag cagtgtcgtc tcgagcgcga gcgcggacca cttcttcgtt ttctcgtacg
    15121 cttcgatcaa ggcccaggca ttctccagcg tcagcgcgag cggaccctcg ctagcggcgt
    15181 gtttcccatt gcggttggcg gccaggcaaa cggccgcgtg gctttggggc ggcgtcgaac
    15241 agatgacggc atcgagcgtt tcttcgccgc acatccgttc gtaatccttt tcgccctttc
    15301 cgtagcgctt ggaggcggcc ttccccgctc ctctcaccag cccggccgct tgctccaggc
    15361 gtgcggtgtc gacgtcgcac agagcagcaa cttccacgcc ctgcaccatc agggctgctt
    15421 tcaggtgata ggtaccccgc gctcccaaac cgacgaatcc gagccgcacc ggctttgtgt
    15481 ttgaactctg cattgtagac atgggctggt tctccttacg atttcattcc cttccggaac
    15541 gcctcttcaa tgcgagggct cgtcttccat ttcccttttg tgaaatcggg aaagtcgacc
    15601 gg
//
LOCUS       DASTMK010000126         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_614861, whole genome shotgun sequence.
ACCESSION   DASTMK010000126 DASTMK010000000
VERSION     DASTMK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614861"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1960)
                     /locus_tag="VFQ79_05180"
     CDS             complement(11..1960)
                     /locus_tag="VFQ79_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139076.1"
                     /translation="MLDMRKAVFLALLIAASAVAQTQPGDVTRYLSTPILPPHVAESE
                     LRMYLMRKVQISPLPARAENWTAEARHLKERILKQIVFHGWPREWVDAPLKFEDLGVI
                     EANPEYRMRRLRYEIVPGMQSTAILYEPANMQGKVPAILNVNGHVGPLGKSIEYKQKR
                     CINQARRGILALNLEWLSFGELAHPENVHWFAAHLDLVGSNSLGLFYLAMRRGLDYLY
                     QHPSVDRSRIGMTGLSGGGWQTIVLSALDERVFAAVPVAGYSSLASRIERPADVGDVE
                     QNATDLLTIADYPLLTAIRAPRPTLLVYNAEDDCCFRAPLVKPYIFDFVRPYFKLYGD
                     ADRLSWHENTDPSTHNYQLDNRQQSYRFFARHFGMPAAVREIPVDAEIKSYDELVVGL
                     PKDNLTILGVAKVLAARIERRGGPSGDTSRSAWVQSQRDLLRQTVRYRPVDVRHAWAV
                     GNTHQRGLETRSYRFEFDNGLSASAVWLQALSAPQEGPATIVLNDDGRKAAGGEVSDR
                     VNRGERVLALDPLFVGDAQPQATGLRTFGQLLAATGDRALGIEAAQVASTGRWIRESG
                     SAKMLRIETSGIRSQVIALVAAVLEPDLFSEVVFRKGMKSLAHLLDAPVEFSNSADLF
                     CLDLYRHFDIEPLTQAATKTRIVWR"
     gene            complement(1962..2159)
                     /locus_tag="VFQ79_05185"
     CDS             complement(1962..2159)
                     /locus_tag="VFQ79_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139077.1"
                     /translation="MEGAIDGRRLRHARQDPAGRNQVDFGKGGFVILGFVKPGSGVEA
                     GLQGRRLSRNRGDLDWLFEEE"
     gene            complement(2202..3449)
                     /locus_tag="VFQ79_05190"
     CDS             complement(2202..3449)
                     /locus_tag="VFQ79_05190"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0139078.1"
                     /translation="MNKQFITLRGHLGAWSTPTQAVRAGDLVLVGGQMSLDERGRAAG
                     SDIATQARNAFESMKCVLAEAGASMSDIVKHNVYFHCNGDAAALERFMDDFNKVRLAY
                     FSDPGPTTTEVQVGLDREGSLILVDAWAVVGANKQRLAPSGHWNWKKRLQFSHGWKVG
                     DMIFIGGQRSLDHEGRLIGSGDIESQTDNAFHNLDTMLREAGGSRHNLMRQNTYYVFF
                     GEGQQVTSYWEKMTNVRRRYMSVPSAAGAGLRISGLPYPDELIQVEGIGILGENKQRL
                     QPANHWDWSIPHSQFTQGWRIGNLAFIGGQISADRNARAVGGDMATQAHNVFAFIRSV
                     LREGGLDESDVIKLYIYYHAPGDWLQVAETTATITRIQRQFYPEPGPAVTAVRVSGFA
                     FEDLLIEIEAIAVCRDESAAAQA"
     gene            complement(3442..>4857)
                     /locus_tag="VFQ79_05195"
     CDS             complement(3442..>4857)
                     /locus_tag="VFQ79_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139079.1"
                     /translation="NEPRAMPDAFFQKHPEIRGAKHMNLYAMCTSVPAVREWLADSLA
                     HVVRHVPDLGGIYCITMSENLTNCFSKGDAWGKKAPIAGDCRRCARRKSWDVIAEVIR
                     AMREGVRRHSKTSDVIAWDWGWGDELTDNVLPLLPKDARVLSEPGIGEFVLSVVDPGP
                     RSLRNWEQARTTGLAPMAKVQFNNTWEISAVPYIPVMHLVLQHCENLAKAAITGLVAS
                     WTCGGYPSPNLALAKPYYFEPRPSKDEILKTEAVRRYGKHAADTAVQGWKQFSDAFVK
                     YPYGIGDVSLTYVVPTQHGPANPLRVRRTGYRAGMILFPYDDYRSWCGRHSPETVQKG
                     FAAMAALWKEGLATFRRALPMVPLHKKRFAELDLAIAETCYYHFQSTANQLEFYILRE
                     KTGDRAARARMRRIAEEEAELARRQFSIARDHSIIAYEASNHYFYTPLDLVEKVLNCQ
                     NVIRELERHRKPTNRRNNANE"
BASE COUNT          945 a         1468 c         1457 g          987 t
ORIGIN      
        1 gcatccccgt tcatctccac acaatgcggg tcttcgtggc ggcttgtgtg aggggttcga
       61 tatcgaagtg cctgtaaagg tcgaggcaaa acaagtccgc gctattcgaa aattcgactg
      121 gcgcatccag cagatgtgcc agggacttca tccccttacg gaaaacaacc tctgagaaga
      181 ggtctggctc aagcacagca gcgacgaggg cgatgacctg gctgcgaatg ccagaggtct
      241 cgatgcgcag catcttcgca ctcccagact ccctgatcca ccgcccggtc gaggcaactt
      301 gcgcagcctc gatcccgaga gcccggtcgc ctgtcgctgc cagcaactgt ccaaaggtgc
      361 gaaggccggt ggcctggggt tgtgcgtcac caacaaatag aggatcgaga gccagcaccc
      421 gctctccgcg gttcacgcgg tcggatacct ccccacctgc ggctttcctc ccgtcgtcgt
      481 tcagcacaat tgtcgcgggc ccttcctgcg gcgccgaaag cgcctggagc catacggcgc
      541 tggcgctcaa gccattgtcg aactcgaagc ggtacgaccg ggtctcgagt ccgcgttgat
      601 gggtgtttcc gaccgcccag gcgtggcgca cgtccacggg cctgtagcga acggtctggc
      661 gcaacaaatc ccgttggctt tgtacccatg cactccgcga cgtgtcgccc gaaggccccc
      721 cgcgccgctc gatccgcgcg gcgagcactt tggcgacgcc gaggatcgtg agattgtcct
      781 tcggcagccc cactacgagt tcgtcatagc ttttaatttc ggcgtccacg gggatttccc
      841 gcacggcggc gggcattccg aagtgccggg caaagaagcg gtaggactgc tgccggttat
      901 ccagttggta gttatgggtg gagggatccg tattttcgtg ccaggagagc cggtcggcat
      961 cgccatacag tttgaagtac ggccggacaa aatcgaagat atagggcttg acgagcggtg
     1021 cgcggaaaca gcagtcgtcc tccgcgttgt agaccagcaa cgtggggcgt ggtgcccgta
     1081 tcgctgtcag caaagggtaa tccgcgatgg tgagaaggtc ggttgcgttt tgttcaacgt
     1141 ccccgacgtc tgccggtctc tcgatacgag aggccaggga ggaatagccg gccacgggca
     1201 ctgctgcgaa gaccctctca tccagagcgc tgagcacgat ggtctgccaa cctcccccgg
     1261 aaagtcccgt cataccgatg cggcttcgat cgacagaagg gtgctggtag aggtagtcca
     1321 gcccccggcg catcgcaaga tagaacaggc cgagggagtt ggaccccacc aggtccaggt
     1381 gtgcggcgaa ccagtgaacg ttctccggat gggcaagctc gccgaaggac agccactcca
     1441 ggttgagcgc caggatgcct cttctcgcct ggttaatgca acgtttttgt ttgtattcga
     1501 tgctctttcc caatggcccg acatggccgt tcacgttgag gatggccggc acttttcctt
     1561 gcatgttcgc cggctcgtac agaatggcgg tggactgcat ccccggtacg atctcgtagc
     1621 gcaatcggcg catccggtac tccgggttcg cctcgatcac gccgaggtcc tcaaacttga
     1681 gcggtgcatc gacccattca cgcggccagc catgaaaaac gatctgtttc aggatgcgtt
     1741 ccttcaggtg ccgagcttca gcagtccagt tctcggcccg ggccggcaag ggagagatct
     1801 ggaccttgcg catcaggtac atgcgcagct ccgattccgc cacgtgggga ggcagaatag
     1861 gggttgagag atagcgcgtc acatctccgg gttgcgtttg ggcaacggcg gatgcggcga
     1921 ttaacagagc gaggaataca gccttccgca tgtcaagcat gctactcctc ctcgaacaac
     1981 cagtcgagat ctcccctatt gcgcgaaagg cggcggccct gaaggccggc ctccacaccg
     2041 ctgccgggct tcacgaaacc gaggatgacg aagccaccct tgccgaagtc aacctggttg
     2101 cgaccggcgg gatcctgacg tgcatgccgg agccgtcgac cgtctatcgc tccctccact
     2161 ccacaaacag gcggccattc gtaggggata tggttgccgg atcatgcctg agcggcagcg
     2221 gattcgtcgc ggcacacggc tatcgcctcg atttcgatca gcagatcttc gaatgcgaaa
     2281 ccggaaacgc gaacagccgt gacggccggc ccgggctcag ggtagaactg ccgctggatg
     2341 cgggtgatag tcgccgttgt ctcggctact tgtagccaat ccccgggagc gtggtaataa
     2401 atgtacagct taataacgtc actttcgtcg agtccgcctt cccgcagcac gcttcggatg
     2461 aaggcaaaaa cgttatgagc ctgtgtcgcc atgtctccgc cgacggcacg cgcgttgcgg
     2521 tcggcggaga tctggccccc aatgaaggca agatttccga tccgccaacc ctgggtgaac
     2581 tgcgaatggg gaatgctcca gtcccagtga ttcgcgggct gcagccgctg cttgttctca
     2641 ccaagtatgc cgatgccctc gacctggatc agttcgtccg ggtacggtaa gccagagatc
     2701 cgtaggccgg cgccagccgc cgacggtacg gacatgtacc gcctgcgcac attcgtcatc
     2761 ttctcccaat agctcgttac ctgctggccc tctccaaaga acacatagta ggtgttctgc
     2821 cgcatcaggt tatggcggct gccgccagct tccctgagca tcgtatccag gttatggaaa
     2881 gcgttatcgg tctgtgattc gatgtcgccg gagccaatta gccggccttc gtgatcgagc
     2941 gatcgctgac caccgatgaa gatcatgtcg ccgaccttcc atccgtgcga aaactggagc
     3001 cgcttcttcc agttccagtg gccagagggg gccaggcgct gtttgtttgc cccgactacc
     3061 gcccacgcgt cgacgagaat cagagatcct tcgcggtcga ggccaacctg tacttcggtc
     3121 gtggtaggac ccgggtcaga aaaatacgcc aggcgaacct tattgaaatc gtccatgaag
     3181 cgctcgagtg ccgcggcgtc gccgttgcaa tggaaataca cattatgttt gacgatatcc
     3241 gacatgctgg ctcccgcttc ggcgagaacg cacttcatcg attcaaacgc gttgcgcgcc
     3301 tgcgtggcaa tgtccgaacc agcagcacga ccgcgctcgt ctagggacat ctggccgccg
     3361 acaagaacaa ggtcgccggc gcggaccgcc tgggttggcg tagaccaggc gccgagatga
     3421 cctcgcaggg tgatgaattg tttattcatt ggcattgttc ctccgattgg tgggcttacg
     3481 gtggcgctcc agttcgcgga tgacgttttg gcaattcaag accttttcga caaggtccag
     3541 cggagtataa aagtagtgat tcgaagcctc gtaggcgatt atcgagtgat cacgcgcgat
     3601 agaaaattgc cggcgtgcta gttcggcctc ttcttccgcg atgcggcgca ttcgcgcgcg
     3661 tgcggcccgg tcgccagtct tttcccggag aatataaaac tccagttggt tggccgtact
     3721 ttggaaatgg taataacacg tctcggcaat agccagatcc aactcggcaa agcgcttctt
     3781 atgcagaggc accatcggga gcgcccgtcg gaaagtggcg agaccttctt tccacagcgc
     3841 cgccatggcc gcaaatccct tctgcaccgt ttccggtgaa tgtctgccgc accagcttcg
     3901 gtagtcatcg taagggaaga ggatcatgcc ggcgcgatag ccggtacgac ggacccggag
     3961 cgggttggcc gggccgtgtt gcgtgggaac cacataagtg aggctcacgt ccccgatgcc
     4021 gtatgggtac ttcacaaacg cgtcgctgaa ctgcttccag ccttggaccg cggtgtcggc
     4081 ggcgtgcttc ccataacgcc gcacggcctc ggttttgagg atctcgtcct ttgacgggcg
     4141 aggctcgaag taatacggtt tcgccagcgc gagattgggt gacgggtagc ctccgcacgt
     4201 ccacgaggcc acgaggccgg ttatggcggc cttggctaag ttctcacagt gttgcagaac
     4261 caggtgcatg acgggaatgt acggaacggc cgagatctcc caagtattgt tgaactgcac
     4321 cttcgccatc ggggcgaggc cggtcgtgcg ggcttgctcc cagttccgca gtgagcgtgg
     4381 gccagggtcc acgacggaca agacaaactc gccgatgccg ggctcgctca gcacgcgcgc
     4441 gtctttcggt agaagtggaa gcacattgtc cgtgagttca tcgccccagc cccagtccca
     4501 ggcaatgacg tccgaggtct tgctgtgccg ccgtacaccc tccctcattg ccctgattac
     4561 ctcggctatg acgtcccaac tcttgcgcct cgcgcagcgt cggcaatcgc cggcaatcgg
     4621 cgccttcttg ccccaggcat cgcctttcga gaagcagttg gtgaggttct ccgacatggt
     4681 gatgcagtaa atcccgccta agtccggcac gtgccgcacg acgtgcgcca agctgtccgc
     4741 tagccattcg cgcaccgccg gaacgctggt gcacatcgca tacaggttca tatgcttagc
     4801 accgcggatt tccgggtgtt tctgaaagaa agcgtccggc atggctcgcg gctcgtt
//
LOCUS       DASTMK010000127         6281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_358681, whole genome shotgun sequence.
ACCESSION   DASTMK010000127 DASTMK010000000
VERSION     DASTMK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358681"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFQ79_05200"
     CDS             complement(<1..464)
                     /locus_tag="VFQ79_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEU0139080.1"
                     /translation="MRADLELLFDQPPPAYTEEHFRLFQRFKDALNQGEIRAAEPDAG
                     AASGWRVNGWVKKGILLGFRMGALVDMSVDATRQPFFDKATYPVKRLSPSSGVRIVPG
                     GSSIRDGCYVGKGVTCMPPMYINAGSYVSEGTMIDSHALVGSCAQIGKSCHIS"
     gene            complement(454..1356)
                     /gene="dapA"
                     /locus_tag="VFQ79_05205"
     CDS             complement(454..1356)
                     /gene="dapA"
                     /locus_tag="VFQ79_05205"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU0139081.1"
                     /translation="MFTGCGTALATPFRRDGSLDEPTLRSLVRRQIEAGINFLVPCGT
                     TGESPTLTRAEHLRVIEITLEEAKGVVPVLGGAGGYNTAEVIELARELAALGVDGVLS
                     VTPYYNKPTQEGLYQHYKAIAGAVRNLPIIVYSVQSRTGVNVEPATLKRLAEIDNIAG
                     VKEASGNLAQVASVVHHVPPEFVVLSGDDAVTLPVIALGGRGIISVAGNEIPGEMTRL
                     AQLALQNDFPAAREIQRRYLPLMEVNFVESNPIPVKAAMALMGLLEPVWRLPLVAPQP
                     ASQAKIEKVLQAVGLLADRGVHAG"
     gene            complement(1374..2063)
                     /locus_tag="VFQ79_05210"
     CDS             complement(1374..2063)
                     /locus_tag="VFQ79_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0139082.1"
                     /translation="MNLAIVGYGKMGRLIEQLAPEYGCQALVKLDEFNNADYAGLTPE
                     NFKGVDVAVEFSTPATVVENIERLSALGVNLVVGTTGWTEHLDRVKDAVARNGTGLVW
                     SPNFSIGVNVFFRLAAEAARLLAPHEQYGAWAWEIHHHTKKDAPSGTLLKLVDGMKAA
                     GYARAVDTASNRAGAHPGTHEIGFDSSADTITLRHTARSREGFARGALTAAQWIAGKK
                     GMYEFSEILFE"
     gene            complement(2065..3435)
                     /gene="lysC"
                     /locus_tag="VFQ79_05215"
     CDS             complement(2065..3435)
                     /gene="lysC"
                     /locus_tag="VFQ79_05215"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HEU0139083.1"
                     /translation="MIVMKFGGSSVESAAAIERVASIVRSRVAMRPVVVVSAMGKTTN
                     RLLAIANDAVHGRRDQALEQLQELRSYHVEESTQLVRDEDRQELDEVIDSHFQELGEL
                     VRGLAILGELTPRSVDAISSFGERLSSHIVTFAFRRCGMKAAHVDSRKVIKTDNRHTH
                     ANPIFPETYNRLSEEIPTFAENGVVVMGGFIGSTMDLVPTTLGRGGSDFSASIVGAGI
                     GADEIQIWTDVDGMLTCDPTLLDGCHRVKTISFAEAAELAYFGAKVLHPATVVPAVEK
                     NIPVLILNSRRPDVEGTRIVADAVHCENVVKSIACKRKITVVNIHSTRMLMTHGFLKK
                     IFEVFERYETPVDMVATSEVSVSLTIDDTERLQSIVDELKRFSEVSVEENQAIVCLVG
                     DNIRFTPGVAARVFSALERVNIRMISQGASKLNLSCVVAQTDLKEAATRLHDEFFSEL
                     DPAVFD"
     gene            complement(3435..4484)
                     /gene="asd"
                     /locus_tag="VFQ79_05220"
     CDS             complement(3435..4484)
                     /gene="asd"
                     /locus_tag="VFQ79_05220"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU0139084.1"
                     /translation="MEQRIEAGVLGATGMVGQHFVKFLQNHPWFELKWVGASDRSAGK
                     KYGEATNWRLDGVMPENISGLTVEESKPGNAPRLLFSAMDASVATEIERAFAEAGHVV
                     VSNSRNHRMEPDVPLLVPEINADHLRIVPVQQRRRGWKGQIVTNPNCSTIVLTMGLAP
                     FKQFGIRRVLATTMQAISGAGYPGVASMDINANVIPFIGGEEEKMQQETQKILGEIGG
                     DEIRYLDASVSAHCNRVPVVDGHMVATSVELREKPDEAAILEAISNFRGVPQERNLPS
                     APARPVVYLEQKDRPQPRRDVERERGMTVFIGRLRPCPVLDFKFMALGHNTVRGAAGA
                     AVLNAELMCSEGMLD"
     gene            complement(4524..5624)
                     /locus_tag="VFQ79_05225"
     CDS             complement(4524..5624)
                     /locus_tag="VFQ79_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0139085.1"
                     /translation="MTGESGGALSRSEALPRIPLPFASYPNPDYIMNVFELTRALIDI
                     ESITGNEKEVGRYLAGLLGELATAFGGSAETMPVEADRFNVLAWWGEPMVTLSTHIDT
                     VPPFFSSSEDSEYIWGRGACDTKGIIASMILAVQGLLEQNTRNVALLFVVGEERNSAG
                     AYFAAQHPLGSRFLINGEPTENRLALGSKGALRYEITAAGRMAHSAYPHLGESAIEKL
                     LDVLEKVRKIPLPVDDLLGPSTLNIGVISGGRAPNIVPDHAKAEVMIRLVEDGESTRK
                     AMQAAVEGKAEAKEILAIPALRLGSLEGFETTVVSYTTDIPAFGGNWGEPFLFGPGTI
                     HVAHTAEERVPKRQLEEAVVLYQSMVKQLLQK"
     regulatory      complement(5590..5817)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         1212 a         1978 c         1802 g         1289 t
ORIGIN      
        1 gagatatggc aactctttcc gatctgtgcg cagcttccca ccagcgcgtg cgagtcgatc
       61 attgtgcctt cgctgacata ggaccccgcg ttgatgtaca tcggcggcat gcaggtgacg
      121 cccttgccga cgtaacagcc gtcgcgaatg ctggagccgc cgggcacgat ccgcacgccg
      181 ctcgaaggac tcagccgttt caccgggtac gttgccttgt cgaagaacgg ctggcgcgtc
      241 gcatcgacgg acatatcgac cagcgcaccc atgcggaaac cgaggagaat gcctttcttg
      301 acccagccat tcacgcgcca tccgctggcc gcgccggcgt ccggttccgc cgcgcggatt
      361 tcgccctggt tcaaagcgtc cttgaaacgc tggaacagcc ggaaatgttc ttcggtataa
      421 gccggtggag gctggtcgaa cagcaattcg agatcagccc gcatgcacac cccggtcggc
      481 aagcagtccc accgcttgca aaaccttttc gattttcgcc tggctggccg gctgcggcgc
      541 caccagcggc aggcgccaca cgggttcgag caaacccatc agcgccatgg ctgctttcac
      601 cgggatcgga ttggactcca cgaagttcac ctccatcagc ggcagatacc gccgctggat
      661 ttcccgcgcc gccggaaaat cgttttgaag cgcaagttga gcgaggcggg tcatctcgcc
      721 tggaatttcg ttgcccgcaa ccgagatgat gccgcggcct ccaagtgcga tcacgggcag
      781 cgtcaccgcg tcgtcgcccg aaaggacgac aaactccggt ggaacgtgat gcaccacgga
      841 cgcaacctgc gccaggttgc cggatgcttc ttttacaccg gcgatgttat cgatctccgc
      901 caggcgcttg agggtagcgg gttccacatt cactcccgtg cggctctgca cgctgtagac
      961 gatgatgggc aggttccgga cagcgccggc gatggctttg tagtgctggt acaggccttc
     1021 ctgcgtgggt ttgttgtagt acggcgtcac cgagagcaca ccgtccacgc ccaaagccgc
     1081 caactcgcgg gcgagctcga tgacctcggc ggtattgtag cctccggcgc cgccaagtac
     1141 cggcactacg cctttcgctt cttcgagcgt gatctcgatc acgcggagat gctccgcgcg
     1201 cgtgagcgtc gggctctctc ccgtcgtgcc gcagggaacc aggaaattga tcccggcttc
     1261 aatctgccgc cgcactaaag agcgcagcgt tggctcgtcc aacgaaccat cgcggcgaaa
     1321 cggggttgcc agggcagttc cgcatcctgt aaacatgtgc cacctccgga gtcctactcg
     1381 aaaagtatct cgctgaattc gtacattcct ttttttccgg caatccattg tgccgcggtc
     1441 agcgcgccgc gcgcaaagcc ctcacggctg cgggccgtgt gccggagagt gatggtgtcg
     1501 gcggaggaat cgaaaccgat ttcatgggtg cccggatgcg ctccggcgcg attcgaagct
     1561 gtatcgaccg ctcgcgcata gccggcggct ttcatcccat ccaccagctt cagcagggtg
     1621 cccgatggag cgtccttctt cgtatggtga tgaatctccc acgcccatgc gccatactgc
     1681 tcatgcggcg cgagaagccg ggccgcctcc gccgcgagcc ggaagaacac gttcacaccg
     1741 atggaaaaat tcggactcca caccaggccc gtgccgttgc gggcgacggc gtccttcacc
     1801 ctgtcgagat gctccgtcca tccggtggtg cccacaacca ggttgactcc cagcgcgctc
     1861 aggcgttcga tgttctccac taccgtggcg ggcgtcgaaa actccactgc tacgtccacc
     1921 cccttgaagt tctccggcgt caatcccgcg tagtccgcgt tgttgaactc atcgagcttt
     1981 accagcgcct ggcatccgta ttccggtgcc agttgctcga tgagccggcc catttttccg
     2041 taaccgacaa tcgccaggtt catttcaatc gaagaccgcc ggatccagct cgctgaagaa
     2101 ctcgtcgtgc agtcttgtag ccgcctcctt caggtccgtc tgggcgacga cacagctcag
     2161 gttcagtttc gacgcgccct gcgaaatcat ccggatgttc acacgctcca gcgcggaaaa
     2221 gacgcgcgcg gcgacgcccg gcgtgaagcg tatattgtcg cccaccaggc agacgatcgc
     2281 ctggttctct tcgacgctga cctcggagaa tcgtttcaac tcgtcgacaa tagattggag
     2341 acgctcggta tcgtcgatgg taagggacac gctgacttcc gaagtggcaa ccatgtcgac
     2401 gggcgtttcg tagcgttcaa atacctcgaa gattttctta agaaagccat gcgtcatcag
     2461 catccgcgtc gaatggatgt tgacgacggt gatctttctc ttgcacgcga tggatttcac
     2521 cacgttctcg caatggacgg cgtcggccac gatgcgggtc ccctcaacgt ccggcctcct
     2581 ggagttcagg atcagcacgg gaatattttt ctccaccgcc ggaacaactg tggcggggtg
     2641 cagcaccttc gcgccgaagt aagccaactc cgccgcttcg gcgaacgaga tggtcttcac
     2701 ccggtggcag ccgtcgagca gagtcggatc gcaggtgagc atgccatcca catcggtcca
     2761 gatctgaatt tcgtcggctc cgatgcccgc gcctacgatg gaggcgctga agtcggagcc
     2821 gccgcggccg agcgtcgtag gcaccaggtc catggtggat ccgataaagc cgcccatcac
     2881 caccacgccg ttctcggcga atgtcgggat ctcttccgac agccggttgt aagtctccgg
     2941 gaaaatcgga ttggcgtgcg tgtggcgatt gtccgtttta atgacttttc gcgaatccac
     3001 atgcgccgcc ttcatgccgc agcggcgaaa ggcgaacgtg acgatatggc tggagagccg
     3061 ctcgccgaaa ctggaaatgg cgtccacgga gcgcggcgtc aattcgccga gaatcgccag
     3121 gccccggacc agctcgccca actcttgaaa atgcgaatcg atgacttcat ccagctcctg
     3181 gcggtcctca tcgcgaacaa gctgggtgga ttcctccacg tggtaagacc gcagttcctg
     3241 gagttgttct aaagcctggt cgcggcggcc gtgaaccgcg tcgttggcga tggcgagaag
     3301 tcgattggtg gtcttaccca tcgcggaaac cactaccacc ggcctcatgg ccacccgcga
     3361 tcgcacgatg gaagctacac gctcgatggc tgccgccgat tccacggacg agccgccgaa
     3421 tttcatgacg atcatcaatc gagcattcct tccgagcaca tcagttcggc gttgagcacc
     3481 gccgcgccgg ccgccccgcg aaccgtattg tgacccagcg ccatgaactt gaaatcgagt
     3541 acgggacagg gccgcagccg gccgatgaac acggtcatgc cgcgttcgcg ttccacatcg
     3601 cggcggggct ggggccggtc cttctgttcc aggtacacca ccggacgggc cggcgcgctg
     3661 ggcagattgc gttcctgcgg tacgccccgg aagttggaga tcgcttcgag gatggctgct
     3721 tcgtccggct tttcgcggag ctcgacggac gtcgccacca tgtggccgtc caccaccggg
     3781 acgcggttgc agtgcgcgct tacactggca tcgaggtagc gaatctcgtc accgccgatt
     3841 tcgccgagga tcttctgcgt ctcctgctgc atcttctcct cttcgccgcc gatgaacgga
     3901 atgacgttgg cattgatgtc catcgaggcc acgccgggat agccggctcc ggaaatcgcc
     3961 tgcatcgtcg tcgccagcac gcgccggatg ccgaattgtt taaacggtgc gagccccatg
     4021 gtgaggacga tggtggagca gttgggattc gtcacaatct gccccttcca tccgcggcgg
     4081 cgctgctgca cgggcacaat gcggagatgg tccgcgttga tctccggcac cagcagggga
     4141 acgtccggct ccatgcgatg attgcgcgaa ttcgatacca ccacgtggcc cgcttcggcg
     4201 aaggcccgct cgatctccgt ggccacggaa gcgtccatgg ccgagaacag aagtcgcggc
     4261 gcgttgcccg gcttcgattc ctccactgtc aggccggaga tattttccgg catgacacca
     4321 tccagccgcc agtttgtggc ttcaccatac ttctttccgg ccgagcggtc gcttgcgcca
     4381 acccatttca gctcgaacca gggatggttc tggaggaatt ttacaaagtg ctgcccaacc
     4441 attccggttg cgccgagcac gccggcttca attctctgtt ccatttcgct tctttctcca
     4501 aattcttgga cggcaacggc cgcctacttc tgcaacaact gcttcaccat cgattgatac
     4561 agcaccactg cttcttcgag ctgccgtttg ggcacgcgct cctccgcggt atgagcgacg
     4621 tgaatggttc cggggccgaa gagaaagggc tctccccaat tcccgccgaa cgccggaata
     4681 tccgtcgtgt aggaaaccac ggtggtttcg aaaccttcga gcgagccgag gcgcagcgcg
     4741 ggaatcgcga ggatctcttt cgcttccgcc ttgccctcca ccgcggcctg catcgccttg
     4801 cgagtggatt cgccgtcttc caccaggcgt atcatgacct cggccttggc gtggtccggc
     4861 acgatgttcg gagcgcggcc gcctgagatc acgccgatat tcagcgtgct ggggccgaga
     4921 agatcgtcca ccggcagcgg gattttccgg accttttcta atacgtccag cagtttctcg
     4981 atcgcggatt ccccgagatg cggataggcc gaatgcgcca tgcggccggc ggctgtgatt
     5041 tcataacgca gcgcgccttt cgaccccagc gccagccggt tctccgtcgg ttcgccgttg
     5101 atgagaaacc gtgagccgag cggatgctgc gccgcgaagt aagccccggc gctgttgcgc
     5161 tcctcgccta caacaaatag gagcgcgacg ttccgggtgt tctgttcaag cagtccttga
     5221 accgcgagaa tcatcgacgc gataatcccc ttcgtgtcgc acgcgccgcg gccccaaata
     5281 tactcactgt cttccgagga agagaaaaaa ggcggaaccg tatcgatgtg tgtggagagc
     5341 gtcaccatcg gttcgcccca ccaggcaaga acgttgaacc tgtccgcctc gacgggcatg
     5401 gtctccgcgg agccgccaaa cgcggtggcg agctcgccca gcagtccggc caggtagcgg
     5461 cccacttcct tctcgttgcc cgtgatcgac tcgatatcga tcagggctcg tgtcagctcg
     5521 aagacattca taatatagtc gggattcgga taggaggcga aaggaagagg aatccgtgga
     5581 agggcttcgc tcctcgatag cgctccaccc gattctccgg tcaacggttt ctccgttgcc
     5641 aaaagacccg gatgacagtg ggaaggattt agccttgccc cccagccgcg gcggcggatg
     5701 ctgcatccag cggcttcggc gggatcgtta ggaatacggc cccttttgcc cgaccctccg
     5761 aagctcccgg agactgccgg tcaggctact catgaccccc gcacctctac caagtaaata
     5821 tcaaaaatag cgaaccgcac ccccggaagt caattcgagg agccgcaccg ccgcaagtaa
     5881 cagcagtgat agcatgccgc ataagcgcgc cgtctgttgc ctctaccgcc aatctgcgcc
     5941 gcaatgcaca actgcccttg gccactctgg aaaatatgct tttattgtgc ggaagaacgc
     6001 aaattttacc ggattattct actctcgaac cgccactcat atagccacgc cttttgtaga
     6061 catagcgata tcaagagaat ggatttcaac tcaaacactc tgcaaaaaat gagatagttt
     6121 ctcccgctcg gcgaaataaa atcgtaatgg aaggcgtatt gctaatttct cagatgtcag
     6181 gaactgcgcg agttgatcaa ggaatcaacg gaggttaatt tatgaatcgt gcgtttgttt
     6241 tgtgtgcgtt tcttttgtgt tcgtcggtat cgagcactgt a
//
LOCUS       DASTMK010000128        10106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_358748, whole genome shotgun sequence.
ACCESSION   DASTMK010000128 DASTMK010000000
VERSION     DASTMK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358748"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..756)
                     /locus_tag="VFQ79_05230"
     CDS             complement(19..756)
                     /locus_tag="VFQ79_05230"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEU0139086.1"
                     /translation="MITLEARAVDLIHGAIETYGDRFAIVTGFQLEGMVLVDIAARIG
                     KTVRVLSLDTGRLPSETFSIAQLIKSRYGIEVEFIKPAAEEVDAMVERYGADLFYSSV
                     AHRMLCCQVRKVRPLERRLQPLAAYAVGLRRGQTEARVDIGHVTEEPGGRLKLAPLAD
                     WTREMVADYTARHHVPQHPLYARGYASIGCEPCTRAIQPGEAERAGRWWWEDGAQKEC
                     GLHFSPDGKAERQVDVLLREILADAHA"
     gene            831..1661
                     /locus_tag="VFQ79_05235"
     CDS             831..1661
                     /locus_tag="VFQ79_05235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0139087.1"
                     /translation="MRLLFVLVLASTVAGAYTVDPVAPRCGDVIQVRGDAVAARMNGR
                     TVPLFAGSGPARVGLMPVPLEEKPGKYPLEFLGTDGRVAGTVQITVREPHYPKQNIVI
                     GKEKKELRPSPGETETVQAFRETVSATRYWKEPFVLPVRGCMTSPFGVARLHNGQPTG
                     SYHRGLDQRGATGTPVRATAAGVVKVARMFNLHGGTIGIDHGQGLTSMYLHLSKLAAE
                     EGAHVEKGAVIGYVGTTGFSTAPHLHWALYAHGVSVSPLQWVQLKPCAAPVRGKKKSR
                     "
     gene            complement(1671..2324)
                     /locus_tag="VFQ79_05240"
     CDS             complement(1671..2324)
                     /locus_tag="VFQ79_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139088.1"
                     /translation="MKKRLSLFTIAATAVLILGGCAATTGTASRTNTSPVYTSSNQPG
                     VIPAGTTLSVRLNETIDSAQPIEGRTFSGEVVREIVDSSGTALVPQGSPVQVYVYDFE
                     KGGTLDASRAKMGVRSLTVGGRTYSVDVLEESRRSEGGLGANRRTAEMVGGGAALGAI
                     IGAVAGGGSGAAIGAAVGAGGGAAAQVITRDKQLRIPAETVLNFQLENPIRLVGYSR"
     gene            complement(2377..2844)
                     /locus_tag="VFQ79_05245"
     CDS             complement(2377..2844)
                     /locus_tag="VFQ79_05245"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU0139089.1"
                     /translation="MDSQFLEEAIRLAVENVRKGVGGPFGAVIVQRGRVIGRGTNLVT
                     STNDPTAHAEIVAIRQACQALGDFQLTDSEIYTSCEPCPMCAGAIYWCRPARVYFAAG
                     HEDASRAGFDDSFIYSDLAVPPEKRTVPFLKVAVPDFLLPFREWEGKSGNARY"
     gene            2892..3716
                     /locus_tag="VFQ79_05250"
     CDS             2892..3716
                     /locus_tag="VFQ79_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU0139090.1"
                     /translation="MAATVWKGFLTFGLISIPLRLHSAARSKRVEFHQLHAPDLTRVK
                     QQLYCPADERVVDRSEIVKGYETAKDHWIVVEDEEAQKVAPPSSETMEILQVVQLEEV
                     DPIYFETSYYSVPEPPGRKAYSLLFQSMKSGGLAAIAKLAMHRREYTVVIRPYRNGLA
                     LHTIYYADEVRGVEEYENLETVRVTSKEIQLAAQLLQNLQAPFKIEAYHDEYRERLER
                     LVEAKANGKTIRAAKPRKMAPVIDLMQALEKSLKRRPQPEKRPRRAITHTKVRKAG"
     gene            3866..4498
                     /locus_tag="VFQ79_05255"
     CDS             3866..4498
                     /locus_tag="VFQ79_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0139091.1"
                     /translation="MKLPDKRTCAILGGLGIGAGLMYLLDPNNGKRRRSIVKDKARKA
                     YHTSGRAAGRWGRKIANRGKGALSEVKARWMEEDVPDSILEARVRSHVGHHVSRTGWV
                     EMTAHNGVIVLTGSLPKQEASTLLAQVEAVRGVKGVDDQLNIQSEGNGRSKGWSRTLG
                     MVASAVGGGLALYGVRKRGALGTALSSVGRGAAKLSGWGQEAVQHQPGER"
     gene            4495..4884
                     /locus_tag="VFQ79_05260"
     CDS             4495..4884
                     /locus_tag="VFQ79_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139092.1"
                     /translation="MKRVLVAAALVAALFSCSRPASTGEDTAQAEDRRRYQEELESKL
                     DRVSRELEDAKKEIRAKWDAWKPEIREQRREALTELERAEADARDKLAKLKTASAEGW
                     AEFRRGVDKAVEDVQAGWKRFKAEKLD"
     gene            5143..5805
                     /locus_tag="VFQ79_05265"
     CDS             5143..5805
                     /locus_tag="VFQ79_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEU0139093.1"
                     /translation="MTVILVLCTFLVFIGIDLWLNRKKAITVAAEPAAAKVPAGENYI
                     EGFLVPSTVRYHAGHSWLHRERKNVIRVGMDEFAAALAGRVEKFELPKPGHWVRQGQK
                     AWSFLRAGEKGEMVSPIEGEIVEINQEVVENPSLVREDPYGKGWLFTVFAPDEESTLR
                     NMLPQNLVSEWMRSSIEKLYGLQPELAGAVAADGGRPAEDLCAALPNVNWKEVTREFF
                     LT"
     gene            5898..6668
                     /locus_tag="VFQ79_05270"
     CDS             5898..6668
                     /locus_tag="VFQ79_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0139094.1"
                     /translation="MARAILFDSTQCVGCKACEEACATKWGNPYDEKVAAEERVSSHK
                     LTAVRTFGEHYSRKLCMHCLEPSCASVCPVGALQKTALGPVVYDESKCMGCRYCMVAC
                     PFQVPSYEWDSRLPRVKKCDGCFERQSKGQPTACTEACPAGATIGGEREELIAEARKR
                     IAENPGQYYNHIYGLEEVGGTSVLMLSAVPFDQIGMPGGLPKDALPERTWAVLSHIPD
                     VAGLGAVLLGGVYWITHRRDEVAKAEREEAGKEKENVR"
     gene            6665..7837
                     /gene="hybB"
                     /locus_tag="VFQ79_05275"
     CDS             6665..7837
                     /gene="hybB"
                     /locus_tag="VFQ79_05275"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HEU0139095.1"
                     /translation="MTGTVRLPRITFWRTVFWFIMAAAVYATYVRFVDGLGSSTNLSD
                     EFPWGIWIGFDVLCGVALAAGGFTLAATVHIFNIEKYKPIVRPAILTAFLGYLLVVVA
                     LLFDLGRPYRIWHPLIMWNPRSVMFEVGWCVTLYSTVLSLEFAPVVFERFGWRAPLRY
                     IKAIMIPLVIAGVILSTLHQSSLGTLYLIFPTKLHALWYSPMLPVMFFVSAIALGLAM
                     TIFESWHSSRAFGRHLELPLLSSLGRVLAVLMLFYLCLRFLDLGKRGALNLLTVGSIE
                     TYLFGLEIALLLTPTFLLFWSRVRRSPVALYLSAVMVVLGMIVNRLNVSITGMERGSG
                     VSYIPAWTEVAVTLGIIAAGFAIFRLAVQHLPIFKEEEQAEPASAPVYQMAGALGD"
     gene            7837..9519
                     /locus_tag="VFQ79_05280"
     CDS             7837..9519
                     /locus_tag="VFQ79_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0139096.1"
                     /translation="MEMGPGGPTTRKVRAPLALKLAVCLVIGTAGFFAFAGYLNLNMQ
                     RRNYERLVLASADRVSDIIQRSTRYQMLLNNREGVYHLIRDVGSEPGIRRVRIFNKEG
                     LISYSTEPGEVGRLVDKRAEECYACHAQEQPLTKLDRPDRARIFKDPGGERILAVIQP
                     IENQPDCSNAACHAHPESKRILGVIDAQLSLRAVDEQMAGHQTQITTLTALAVLLFSA
                     LSAVFIWGVVHRPIKELMAGTKKVAGGELDLRLPVRSHDELGALAQSFNKMTSELEQA
                     HAELTEWARTLEDRVRIKGEALERAHSSLVASEKMASLGKLAATVAHEVNNPLFGMLT
                     YARLTIKTLQAGDIDPKARAAMIENLKIIERESLRCGEIMKNLLTFARQQRPHRAPTD
                     LNTLVDRAVKLVRHKLEMQDIDLEVTLDPAIPEIRCDSGQIQQVILVLTVNAAEAMPQ
                     GGRLTMATACDTTKRTAEVRVRDTGSGIPPEALNKIFEPFFTTKEDQHRTGLGLAIAK
                     NIVEQHAGSLAVESQPGKGTEFVLTLPFDVPEPDTPALSAAGGSGNSEKEEA"
     gene            9516..>10106
                     /locus_tag="VFQ79_05285"
     CDS             9516..>10106
                     /locus_tag="VFQ79_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0139097.1"
                     /translation="MNNAAVKAGARILIVDDDPVVRDSLGKWFESEGFQATTLPGARE
                     VLEQIQNSRWDAALIDIKMPGIDGMELQQRLREIDPELPVIIMTGYASIDTAVKALKR
                     GAYDYITKPFDPDELVHLVSNAVEHRWAKREVVRLRENLQEVFPNTRLIGQSEPMKRV
                     IESIETVAPTDATVLITGESGTGKEIVARAVHAGSPR"
BASE COUNT         2108 a         2803 c         3193 g         2002 t
ORIGIN      
        1 aaccacagcg tgaatccttt atgcatgcgc gtctgcgaga atctcccgga gcagcacgtc
       61 gacctggcgt tcggctttgc cgtccggcga aaagtggagg ccgcattctt tttgcgcgcc
      121 gtcttcccac caccagcggc ctgcacgctc tgcttctccc ggctggatag cccgcgtgca
      181 cggctcacag cctatactcg cataacctcg ggcatacaac ggatgctgtg gtacgtggtg
      241 gcgcgcggtg tagtccgcta ccatctcgcg tgtccagtcg gccagtggtg ccagtttcag
      301 gcggccgccc ggttcctcgg tcacgtgacc gatatcgacg cgtgcctcgg tctgtccgcg
      361 ccgtaagccc acggcgtacg cggccaacgg ttggagccgc ctttcgagcg gccgcacttt
      421 acggacctgg cagcacagca tcctgtgcgc aaccgaagag taaaacaggt cggccccgta
      481 gcgttccacc atcgcgtcaa cttcttccgc ggcgggtttt ataaactcga cctctatgcc
      541 gtaccgcgac tttatgagtt gcgcaatcga gaaggtctcg gagggaagcc ttccggtgtc
      601 caggctcagt acccggacgg ttttgccgat tcgcgcggcg atatccacca acaccatccc
      661 ttccagttgg aaaccggtca cgatggcgaa ccggtcgcca tatgtctcta tagcaccgtg
      721 gataaggtcg actgcgcgtg cttccagagt aatcacccta atgcctatta ggataggggt
      781 ttttgaggct attcgcaact gtcctgcccc gccgcgataa gctgtaaaca atgaggctac
      841 tgtttgttct ggtcttggct tccaccgtcg cgggggcata cacggtggac ccggttgcgc
      901 cgcgctgcgg cgatgtcatt caggttcgcg gcgatgcggt cgcggcgcgg atgaacgggc
      961 gcactgttcc gctgtttgcc ggctccggcc cggccagagt gggactgatg cccgtaccgc
     1021 ttgaggagaa gccagggaag tatccgctcg agtttttagg aaccgacggg cgggttgcag
     1081 gcaccgtcca gataacggtg cgtgagcctc actacccgaa gcagaacatt gtcatcggga
     1141 aagaaaagaa agaactgcgc ccgtctcccg gagaaaccga gacggtgcag gcctttcgcg
     1201 aaaccgtgag cgcgacccga tattggaagg agccttttgt cctgccggtg cgtggatgca
     1261 tgacctctcc ttttggtgtg gcacgcctgc acaacgggca gccgacgggc agctatcacc
     1321 gcgggctcga tcaaaggggc gccacgggga cgcccgtccg ggcaacggcg gcgggcgtcg
     1381 tcaaagtcgc ccggatgttc aatctgcacg ggggcacgat cggaattgac cacggacagg
     1441 gcctgacatc gatgtacctg catctgtcga agctggcggc ggaagaaggc gctcacgtcg
     1501 aaaaaggagc cgtcatcggc tacgtgggca ctaccggttt ttcgacggct ccacacctgc
     1561 actgggcctt atatgcgcat ggcgtttcgg tgagcccgct gcaatgggtt caactgaaac
     1621 cttgcgcggc gccagtgcgt ggcaaaaaga aaagccgtta agcccctggt ttaacggctg
     1681 tagcctacca ggcgaatcgg attctccaac tggaaattca aaacggtttc ggcgggaatg
     1741 cggagctgct tgtccctggt gatcacctgt gcggccgctc cgccgccggc tcccactgct
     1801 gctccgatcg cagcacccga gccgcctccg gctacggctc cgatgatggc gcctaaagcg
     1861 gctccaccgc cgaccatttc ggctgttcgc cggttcgcgc ccaggccgcc ttcactgcgg
     1921 cggctttctt ccagaacgtc cacggagtag gtccggccgc ctaccgtgag agaacggacg
     1981 cccatcttcg ctcgcgaagc gtcgagtgtt cctccctttt cgaagtcgta gacatacact
     2041 tgcacaggcg aaccctgcgg caccagcgcc gtacccgaag agtcgacgat ttcgcgaaca
     2101 acttcaccgg aaaaagtgcg gccttcgatg ggttgggcgc tatcaatggt ttcgttcagc
     2161 cggacggaaa gcgtagttcc tgccggtatc acgccgggtt gattggaact cgtgtaaacc
     2221 gggctggtat tcgtacgcga ggccgttccg gttgttgccg cgcatccgcc cagaatgagc
     2281 acggcggttg ccgctatcgt gaatagcgat agtctttttt tcatacggtc tgctcctccg
     2341 ccaatcgggg agcaaccggc ttgccagggc gcgaaatcaa tatcgagcgt tgccgctctt
     2401 gccttcccat tcacgaaaag gaaggagaaa gtccggtacc gctactttca ggaacggcac
     2461 ggtccgtttt tccggcggca cagctaagtc gctataaatg aaggagtcgt cgaacccggc
     2521 gcgggatgcg tcttcgtgcc ccgcggcgaa atagacccgg gccggccggc accaatagat
     2581 agcgcccgcg cacatggggc agggttcaca ggaggtataa atttcgctat cggtgagctg
     2641 aaaatccccg agagcttggc aagcctgccg gatggcgacg atttcggcat gggcggtggg
     2701 gtcgttggta gaagtgacga ggttcgtccc ccggccgatg acgcgtcccc gctgaacgat
     2761 aaccgctccg aacgggcctc ccacgccttt tctgacattt tccacagcca gacggatcgc
     2821 ctcttccaga aattgcgagt ccatgcgcaa tagtttacag cgtgatgcat ccataaatgc
     2881 ggagagatgg aatggctgca accgtttgga agggctttct cacgttcgga ctgatctcga
     2941 ttcccctgcg gctgcattcg gcagcgcgaa gcaagcgtgt tgaatttcac caattgcatg
     3001 cgcccgacct cacgcgggtg aagcagcagc tttattgtcc tgccgacgag cgcgtggtcg
     3061 accgctcgga gatcgtgaaa ggatacgaaa cggcgaagga ccactggatt gtagtggagg
     3121 acgaagaggc gcagaaggtg gcgccgcctt cttccgagac catggaaatt cttcaggtcg
     3181 tgcaactgga ggaagtggac cccatctatt tcgagacttc ctattactcc gtaccggagc
     3241 cgccgggccg gaaagcgtac tcacttctgt tccagagcat gaaatcgggc ggcctggccg
     3301 cgattgccaa gctcgccatg caccggcgtg aatatacggt tgtgatccgc ccttatcgaa
     3361 acggactggc gcttcatacc atctactatg ccgatgaggt ccgcggcgtc gaagagtacg
     3421 agaacctgga aacggttcgc gtgacctcga aagagattca gcttgccgca caactgcttc
     3481 agaacctgca ggcgcccttc aagattgagg cgtatcacga cgagtaccgc gagcgtctgg
     3541 aacgcctggt ggaagcgaaa gcgaacggca agacgattcg ggcggccaaa cctcgcaaaa
     3601 tggcgcctgt gatcgacctt atgcaagcgc tcgaaaagag cctgaaacgc cggcctcaac
     3661 cggaaaaacg gccccggcga gccataacgc acaccaaggt tcggaaggcg ggttgacgat
     3721 tgaacgggaa acgttcagcg gacggatgga gtcccacatt aagaccgctc cctcacggtc
     3781 acggctccga accgagccgc gcgcgggttg cggtattacc gtaattccga atagaggtac
     3841 taaggacaca ggaggagatg aggagatgaa gctgccggac aaacgaactt gcgcaattct
     3901 cggaggcctc ggaataggcg ccgggttgat gtaccttctg gaccccaata acggaaagcg
     3961 caggcggtcc attgtcaagg ataaggctcg gaaagcgtac catacctcgg gccgggcggc
     4021 cggacgctgg gggcggaaga tcgcgaaccg ggggaaggga gccctgtcgg aagtgaaggc
     4081 ccggtggatg gaagaagatg tcccggacag cattctggaa gccagggttc ggtcacacgt
     4141 gggacatcat gtctcccgga cgggttgggt ggagatgacc gctcacaacg gtgtgattgt
     4201 tctgacggga tcgctaccta agcaggaagc ttcgacactg ctcgcccagg tggaagcggt
     4261 gcgcggcgta aagggcgtgg acgaccaatt gaacattcaa tcggaaggga acggacgctc
     4321 gaagggctgg tcccgaaccc tgggtatggt cgccagcgcc gtgggtggcg ggcttgccct
     4381 gtatggggta cggaagcgcg gtgctctggg taccgccctt tccagcgtgg gccggggggc
     4441 ggcgaaactt tccggctggg gccaggaggc ggtgcagcat cagcccggag aacgatgaag
     4501 cgcgtgctgg tagccgctgc tttggtagcg gcactttttt cctgctcgcg cccggcaagt
     4561 acaggtgaag ataccgcaca ggcggaagat cggcgcaggt accaggagga attggaatcc
     4621 aagttggatc gggtgagccg ggagttggaa gacgccaaga aggagattcg ggccaagtgg
     4681 gatgcctgga agccggaaat ccgcgaacag cggcgggagg cgctgaccga gttggagcgg
     4741 gcggaggcgg acgcacgcga caagctggcc aagttgaaaa cggcttccgc ggagggctgg
     4801 gccgaattcc ggcgtggggt agataaggct gttgaagatg ttcaggcggg ttggaagcga
     4861 ttcaaggcgg agaagctgga ttagatgtag tgaaacacta catgcctgta ggaaaattca
     4921 accaagcatc ctgttccttc attttcaacc actaaggcgt cgatgttgcc ctttgccaca
     4981 ttgttgctgt tgtaattctc cacattgcct accgttgatg aactccacaa ttacctgtaa
     5041 accattgatt taaaatatta ttatacaatg gttttgcgat tgctacctta aagtgtgaag
     5101 tttcaggaga ttaagacctg aaacagaaat gagggtagcc atatgacagt cattttagtg
     5161 ctttgcacgt ttctggtatt catcggcatc gacctctggt tgaaccgtaa gaaagcgatt
     5221 accgtcgcgg ccgaaccggc agccgccaag gtgcccgccg gcgagaacta cattgaagga
     5281 ttccttgtcc cttcaactgt aaggtatcac gcgggtcaca gttggctgca ccgggagcgc
     5341 aaaaacgtca ttcgtgtcgg tatggatgag ttcgccgccg ctcttgccgg acgggtcgag
     5401 aagttcgagt tgccgaagcc gggtcactgg gttcgccagg gacaaaaggc atggtcattc
     5461 ctgcgggcag gggagaaggg cgaaatggtg tcgcccatcg agggggaaat cgtggaaatc
     5521 aaccaggagg tggtggaaaa tccttcactg gtgcgagagg acccgtacgg aaaaggttgg
     5581 ctgttcacgg tattcgcacc cgatgaagaa agtacccttc gcaatatgct gccgcaaaac
     5641 ctggtttccg agtggatgcg gagctccata gagaaactgt acgggttgca gccggaactg
     5701 gctggagctg tagcagccga cggcggccgg cccgcggagg atctgtgcgc cgctctcccg
     5761 aacgtcaact ggaaagaagt cacaagagaa tttttcctca cttaacagcg cgttttagtg
     5821 ttgcagaaat aaacaggaga aagagataat gatatccaga tggatgtttc agcccacgaa
     5881 agctagagga gaacggaatg gctagggcga tcctgtttga cagcacgcag tgtgtcgggt
     5941 gcaaagcctg tgaagaggcg tgcgccacta aatggggcaa tccttacgat gagaaggtcg
     6001 ccgcggaaga gcgcgtttct tctcacaagc tgaccgcggt ccggacgttt ggcgagcact
     6061 actcgcggaa attgtgcatg cattgtctgg agccgagttg cgcttcggtc tgtccggttg
     6121 gtgctctcca gaagacggcg ctcggccccg tggtctatga cgagtcgaag tgcatgggtt
     6181 gccgctattg catggtggcg tgcccattcc aggtgccgtc ctacgagtgg gacagccgtc
     6241 tgccccgggt gaaaaagtgc gacggttgtt tcgagcgcca gtccaagggc cagcccacag
     6301 cgtgcaccga ggcatgtccc gcgggtgcga ccatcggcgg ggagcgcgag gaactgatcg
     6361 ccgaagcgcg caagcgaatc gccgagaacc ccgggcagta ttacaaccac atctacggat
     6421 tggaagaagt gggcggtacc tcggtgctga tgctctcggc ggtccccttt gaccagatcg
     6481 gcatgccggg aggattgccg aaagacgcgc tcccggaacg tacctgggcg gtgttgtcgc
     6541 acataccgga tgtcgccggg ctcggagccg tattgttggg cggagtttat tggattaccc
     6601 accggcggga tgaagtggcc aaagccgaga gggaagaagc gggcaaggag aaggagaacg
     6661 tgcgatgaca ggcacagtgc gtcttccgcg catcactttt tggcggaccg tattctggtt
     6721 cattatggcg gcggctgtct atgccactta tgtgcggttt gtcgatgggc tcggcagctc
     6781 cacgaatctc tcggatgaat ttccgtgggg catttggatc ggcttcgacg ttctctgcgg
     6841 ggttgcgctc gcagcgggcg ggtttacgct ggccgcgacg gtccatatct tcaatatcga
     6901 gaaatacaag ccgattgtgc gcccggcgat cctgacggcc tttctgggct acctcctcgt
     6961 cgtggtcgcg ctcttgttcg acctcgggcg gccgtaccgg atctggcacc ccctgatcat
     7021 gtggaatccg cgctcggtaa tgttcgaggt gggatggtgc gtaacgctgt acagtacagt
     7081 gctttcgctc gagtttgccc cggtggtatt tgaaagattc ggttggcggg cgccgctacg
     7141 atatataaaa gccatcatga tcccgctggt aattgccgga gtgattcttt cgacgctgca
     7201 ccagtcctct ctcggtacgc tctatctcat cttcccgacc aaattgcacg cgctgtggta
     7261 ctcgcccatg ttgccggtga tgttctttgt cagcgccatc gcgctcggcc tggcgatgac
     7321 gatcttcgag tcctggcata gcagccgggc gtttggccgc cacctggaat taccgttgtt
     7381 gagcagcctg gggcgtgtgc tcgccgtact catgctgttt tacctttgcc tgcgtttcct
     7441 tgatttagga aaacgcggcg cgctgaacct tttgacggtg ggcagcatcg agacctacct
     7501 gtttggcctc gagatcgctc tgcttctgac tccgacattt ctgcttttct ggtcgcgcgt
     7561 gcggcgaagc ccggtcgcgc tgtacctctc ggccgtaatg gtggttctcg gaatgatcgt
     7621 caaccggctg aatgtcagca tcaccggaat ggaacgggga tcgggtgtga gctacattcc
     7681 ggcatggacg gaggttgccg ttacgctggg catcatcgcc gcgggatttg cgatcttccg
     7741 gcttgccgtc cagcatttgc cgatcttcaa agaggaggaa caggcggaac ccgcttccgc
     7801 acccgtgtat cagatggcgg gcgccttggg agactgatgg aaatggggcc gggcgggccc
     7861 acaacgagaa aagttcgcgc acctctcgca ttaaaactcg cggtgtgcct ggtcatcggc
     7921 acggcgggtt tttttgcgtt tgccggatac ctgaacctga acatgcagcg gcgcaattac
     7981 gaaaggctgg ttctggcgag tgcggatcgt gtcagcgata tcatccagcg cagcacgcgc
     8041 taccagatgc tgctgaacaa tcgcgagggc gtctatcatc tcatccgcga tgtgggaagt
     8101 gagcccggca tccggcgggt gcgaattttc aacaaggaag gtttgatcag ctactccacc
     8161 gaacccggag aagtaggccg gctggtggat aagcgcgctg aggagtgcta tgcctgccat
     8221 gcgcaggaac agccgctgac caagctggac cgtcccgacc gggccaggat cttcaaggat
     8281 ccgggcggcg agcgcatcct ggccgtgatt cagcctatcg agaaccagcc cgactgctcc
     8341 aacgccgcct gtcacgctca tccggaatcg aagcgcatcc ttggtgtcat tgacgcgcag
     8401 ctctcgctcc gcgcggtaga cgaacagatg gccgggcatc agacccagat cactacgctc
     8461 acggcgctgg cggtcctgct gttttccgca ttgagcgccg tgttcatctg gggtgtggtc
     8521 catcggccga tcaaagaatt gatggccggt acgaagaagg tggccggagg ggaactggat
     8581 ctacggctgc cggtgcgttc gcacgacgag ctcggcgcgc tggcgcagtc tttcaataag
     8641 atgaccagcg agttggagca ggcccatgcg gaattgacgg agtgggcgcg cacgctcgaa
     8701 gaccgcgtac ggataaaagg ggaagcgctg gaacgggcgc attcgtcgct ggtggcgagc
     8761 gagaaaatgg catcgctcgg caaactggcc gctaccgtgg cgcacgaggt caacaatccg
     8821 ctctttggca tgctgaccta tgccaggctt accatcaaga ccctgcaagc gggcgacata
     8881 gacccgaagg cgagggccgc gatgatcgag aatctgaaaa tcatcgagcg ggagagcctg
     8941 cggtgcggcg aaatcatgaa gaacctgctg acctttgcgc gccagcagcg gccgcaccgg
     9001 gcgccgaccg atctgaatac gctggtagat cgcgccgtaa agctggtacg ccataagctc
     9061 gaaatgcagg acatcgatct ggaagtaacg ttagatcccg cgattccgga aatccgttgc
     9121 gactccgggc aaatccagca ggtgatcctg gttctgacgg tgaacgccgc cgaggcgatg
     9181 ccgcagggcg gccgtctgac gatggctacc gcgtgcgaca ctacgaaacg taccgcggaa
     9241 gtcagagtcc gcgatacggg gagcggcatt ccgccggaag ccctgaacaa gatcttcgag
     9301 ccgtttttca ccaccaaaga agaccagcac cgtacgggtc tcgggctggc cattgcaaaa
     9361 aacatcgtgg aacagcatgc cggcagcctg gcagtggaat cgcagcccgg caaaggaacc
     9421 gagttcgttc tgacgctgcc tttcgatgtg ccggaacccg acacccctgc gctcagtgcg
     9481 gccggaggaa gcgggaattc cgaaaaggag gaagcgtgaa taatgccgcg gtgaaagctg
     9541 gcgcgcgaat actgattgtg gatgacgacc ctgtcgtccg ggattcgctc gggaaatggt
     9601 ttgagagcga aggcttccag gccaccacgc tgccgggggc gcgggaggtg ctcgagcaga
     9661 ttcagaatag ccgctgggac gccgcgttga tcgacatcaa aatgcccggg atcgacggca
     9721 tggagttgca gcagagattg cgcgaaatcg atccggagct gccggtcatc atcatgaccg
     9781 gctatgcctc tatcgacacg gcggtcaaag cgctgaagcg cggcgcctac gattacatca
     9841 ccaagccgtt cgaccccgac gagcttgtgc acctggtgag caatgcggtg gaacaccggt
     9901 gggcgaaacg tgaagtggta aggcttcgag agaacttaca ggaggtgttt ccaaataccc
     9961 ggctcatcgg ccagagcgag cccatgaagc gcgtgattga atcgattgaa acggttgcgc
    10021 cgacggatgc caccgtgctg atcactggtg agagcggcac ggggaaggag attgtggctc
    10081 gcgccgttca cgccggcagc ccgcgg
//
LOCUS       DASTMK010000129         6362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_295165, whole genome shotgun sequence.
ACCESSION   DASTMK010000129 DASTMK010000000
VERSION     DASTMK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295165"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..629)
                     /locus_tag="VFQ79_05290"
     CDS             complement(39..629)
                     /locus_tag="VFQ79_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0139098.1"
                     /translation="MCSREITLDMHNEPTRLDETSRWMGVVRRAKAGDAAAFEEILIA
                     HERPVLRLALRLLGSLEDAQDAAQDVFLRLHRHLGRFDEERDLSPWLYTMTVNVCREL
                     GRKRKPHVQLQDVGRESPVESAVVDEERRRLLGVALRGLPRKERAALVLRDLEGHSTK
                     DVAAMLRTSEATVRSQVASARLRLREIVERLTRRRS"
     gene            710..1591
                     /locus_tag="VFQ79_05295"
     CDS             710..1591
                     /locus_tag="VFQ79_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0139099.1"
                     /translation="MNRRQLLTASLAAAAARPMPAAEWKHDWHLRYAPRIGFVPDTPI
                     PRQLEIYADYGFRAFEYNGLPQHSNAEIQTFRKKMDELKMSMGVFVVNSGGWKGDAMV
                     DPRFHPKFLADVKRAVEIHNIIGNEAATVTSGLASKSLSHEQQTKNSIEVLKRAADIV
                     DKTKMLLVLEPLNIRVDHAGYFVVFSEHAHQIISGVNHPNVRILFDIYHQQISEGNII
                     NHIHKYWDWIGYFQVGDVPGRREPFTGEVNWTNVFKAIHEKGYKGILGMEHGLSVRGM
                     EGVKKCFEAYRKADTWT"
     gene            complement(1667..1939)
                     /locus_tag="VFQ79_05300"
     CDS             complement(1667..1939)
                     /locus_tag="VFQ79_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139100.1"
                     /translation="MPCIVALLALLFPRIVLVLVFFLTNYIQKAFPDSLLWPLLGFIF
                     LPLTTLVYAWAINVAGAITGVYLVVMILAVLFDLGLLGGSAKSRRD"
     gene            2017..2895
                     /locus_tag="VFQ79_05305"
     CDS             2017..2895
                     /locus_tag="VFQ79_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0139101.1"
                     /translation="MKHPISGVSMQLLAIGAVFGTGALLISGGGCAQAQEEKKNRTAP
                     AGYDDTPFLPDQKWRVHDIKRPHPRVVTPAADDCRHQAPSDAIVLFDGKDLSKWYARG
                     RGANRGKMIEPRWKVQDGYMEIAGGTGDLISKEKFGNAQFHIEWAAPAEIDGTSQWRG
                     NSGVVIMGNYEIQVLDSYDNPTYADGQAASLYGQWPPLVNASRKPGEWQTFDIVFEAP
                     VFEGDKLAKPAYATVFHNGVLMHHRQQFIGRMAHRIVRPYAPHGAEEPLVLQDHDTKV
                     RYRNIWVRKLTGYDQP"
     gene            complement(2914..4131)
                     /locus_tag="VFQ79_05310"
     CDS             complement(2914..4131)
                     /locus_tag="VFQ79_05310"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0139102.1"
                     /translation="MTNFLKRLLSRRDLFGTGAVAALAPLTRAESKPLYHESIYTRLL
                     GVRPLLSCRGHTTIYGGSLMPQEVLRAMEEANDQFVDMYELNAAAGRRIAEVMKTEAA
                     LVTAGSFSAMLLGAAACLTGTDREKIAALPHPAWDRKECLIPKGHRVVYDRAYRAAGM
                     TIVEVENRDQLTNAIGGKTAMLAGLGSTERSRRPDIIPLEELIGIGRKAGVPVLIDAA
                     SELPPVSSLTHYTTMGADLTVISGGKGLLGPQSTGILAGRKDLIEAAALNHSPNANIG
                     RGMKVGKEEIVGLIAALNRYVDLDHETVMETWNRKARFMADQLKDVPGVKAEYRINGA
                     GFGEMRLTWDRNIIPLTGKEAFRKLMSGEPRILYYEDDEGGTFQTRCMKDGDEILAAR
                     RLRQFFLEEARKA"
     gene            4131..5489
                     /locus_tag="VFQ79_05315"
     CDS             4131..5489
                     /locus_tag="VFQ79_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0139103.1"
                     /translation="MHRLPIGVSIPEAYTVWPPYHIFMWRLAGSIILLCSTSALLRAE
                     VRKLTLKQTVGIAMKQNPDIVSARLDEQQAEQAVRIAKDPFIPKVFVGSGAAYSYGYP
                     MSIEGSAPSVFQARAVASIFNRPLSYQVAQAKEEARGRTLDAASRSEEVAMRTTSLFL
                     DAERAARAADIARRQVESARKVAEETAARVKEGRELAIESRRAELNVAVARQHAQELQ
                     GQQEYAQRSLAMVLGFDPQDQVEPAADERALPEPPGSEESAAEIALRNNREVKRLESS
                     LLAKGYEVRANKAAWLPTVDLVAQYGLFSKFNNYEDFFRKFQRHNGQIGVSFQVPIYA
                     GAASSARTARAELEVSRIRVEVNRARGRIALEARQSYQQLENAKTAAEVARLDLEVAR
                     EQLSILLARMQEGRVSMREVESARYTENEKWIAFLNAQYAVERAAYELAWRTGSLMGL
                     LL"
     gene            5596..>6362
                     /locus_tag="VFQ79_05320"
     CDS             5596..>6362
                     /locus_tag="VFQ79_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0139104.1"
                     /translation="MMSRKLLLCLTLLLAFTACNRDPKVVSKKYVDSGNKYFEKGKYK
                     EAALMYRNALKKDGLNGEAYYRLGLTGLRMSRPGEASRALRRAVELQPANVDAAARLA
                     DIYLAAYLAQPQHPRELLEETQQIADRLLKRDPKLFEGLRLAGYLGLARQDLPKALEY
                     FRQAHEVKPYQPDVSLAMMQTLSASNQFDEAEKLAKTAIEKNKEYPQLYDGLYMEYLR
                     RNRQAEAEEILKLKIRNNPTSTESRVQLAGFYAATNRR"
BASE COUNT         1334 a         1895 c         1917 g         1216 t
ORIGIN      
        1 cacccagagc gcgatctcca tttcgtaatc tttgcaattc atgaccttct cctcgtgagg
       61 cgttccacga tttcgcgcag tctcagccgc gcggatgcca cctgtgagcg taccgtcgct
      121 tccgacgtgc gaagcatcgc cgcgacgtct tttgtcgaat gtccttccag gtcccgtaag
      181 acaagcgccg cccgctcttt ccgcggcaga ccacgcagcg cgacgcccaa cagacggcgc
      241 cgctcttcgt ccaccaccgc gctctctacc ggcgattcgc ggccaacgtc ctggagttgt
      301 acatgcggct ttcgcttccg gcccaactcg cggcagacgt tgaccgtcat cgtgtagagc
      361 cagggcgaga ggtcccgttc ctcatcgaac ctgcccaggt gccggtgcag acggagaaac
      421 acgtcttgcg ccgcatcctg tgcatcttcg aggctgccca atagccgcaa tgccagccgg
      481 agcaccgggc gctcatgcgc gatcaggatt tcctcgaatg cggcggcatc ccccgccttc
      541 gcccgccgca caactcccat ccaacggctg gtctcatcaa gccgcgtggg ctcattgtgc
      601 atgtcgagcg tgatctcccg ggaacacatc cgtcacagtt atatactgaa ccggcgcccg
      661 aaacgtggaa agaacttgca gtttttttgt ggtatagctt tggtcaagta tgaatcgacg
      721 tcaattgctc accgcatcgc tggcggctgc cgccgcccgt cccatgcccg ccgcggaatg
      781 gaagcacgac tggcacctgc gttacgcgcc ccgcatcggc ttcgtccccg atactccgat
      841 tccacgccag cttgagatct atgccgatta cggcttccgc gcgttcgaat acaacggtct
      901 gccgcagcat tcgaatgccg agatccagac gttccgcaaa aagatggacg agttgaagat
      961 gagcatgggc gtatttgtcg tcaattcggg cggatggaaa ggcgacgcca tggtcgatcc
     1021 ccgttttcat ccgaagttcc tggcggacgt caagcgggcg gttgaaatcc acaacattat
     1081 cggcaacgaa gccgccactg tcacttccgg tcttgcttcc aagtccctgt cccacgagca
     1141 gcagacgaag aactccatcg aagtgctgaa acgcgccgcg gatatcgtcg ataaaaccaa
     1201 aatgctattg gtgctggaac ctttgaacat ccgtgtggat catgccggat atttcgtcgt
     1261 attctccgag cacgcccacc agatcatcag cggcgtcaat cacccgaatg tgcggattct
     1321 cttcgacatc tatcaccagc agatttccga aggcaacatc atcaaccaca ttcacaagta
     1381 ctgggactgg atcggctatt ttcaagtggg cgacgtgccg ggccgccggg agccgttcac
     1441 cggggaagtc aactggacca acgtattcaa agccattcat gagaagggct acaaaggcat
     1501 cctcggcatg gagcatggac tgagcgtccg cggcatggag ggcgtaaaga aatgtttcga
     1561 agcctatcga aaggcggaca cctggaccta agagcttgtt gaaaaacgcg ttgcagccac
     1621 cgctcagaaa cggagccgcg cgcgtcagca agcggttgac ggtgcattaa tcccgccgcg
     1681 actttgcact gccacccagg agcccgaggt cgaaaagcac cgcgagaatc ataacgacca
     1741 ggtacactcc tgtaatcgcc cccgccacgt tgatggccca ggcgtagacc agcgttgtca
     1801 ggggaagaaa aataaacccg agcagcggcc atagcagcga atcggggaat gctttctgaa
     1861 tgtagttcgt caagaagaac accagcacga gaactatacg ggggaagagc agggcgagca
     1921 acgcgacgat gcaaggcatg gttgtcatta tgcaacgccg catccacaag ggaaaccggc
     1981 gggcgctatt ctgtaaggtt agtttgaggg aaggtcatga aacatcccat atccggtgta
     2041 agcatgcagt tgctggctat tggcgccgtt ttcggtaccg gcgcgttgtt gatttcgggt
     2101 ggaggctgcg cacaggccca ggaagagaag aagaaccgga ccgctcccgc cggctatgac
     2161 gacactccgt ttctgccgga ccagaagtgg cgtgtgcacg atatcaagcg gccgcatccg
     2221 cgtgttgtga caccggccgc ggacgactgc cggcaccagg cgccgtccga cgcgattgtt
     2281 ctattcgatg gaaaggacct gtcgaagtgg tacgcgcgcg gccgcggcgc caaccgcggc
     2341 aagatgatcg agccccgctg gaaggtgcag gacggttaca tggagattgc cggcggcacg
     2401 ggcgatctca tcagcaaaga aaagttcggc aacgcgcagt ttcacatcga atgggcggct
     2461 ccggcggaaa tcgacggcac cagccagtgg cggggcaaca gcggcgtggt gattatgggc
     2521 aactatgaaa tccaggtgct tgactcttac gacaatccca cctatgccga cggccaggcc
     2581 gcttccctct atgggcaatg gccgccgctg gtaaatgctt cccgcaagcc cggggaatgg
     2641 cagacgttcg acatcgtctt cgaagcgcct gttttcgaag gcgacaagct cgcgaaaccg
     2701 gcgtatgcca cggtgtttca caacggcgtg cttatgcatc accggcagca gttcatcggc
     2761 cggatggccc atcgcatcgt gcgtccctat gctccgcacg gcgccgagga accgctggtg
     2821 ttgcaggatc acgatacgaa agtgagatac cgcaacatct gggtgcgcaa gctcaccggc
     2881 tacgatcagc cctgacggcg ccgcccgcgg gcgtcaggcc ttgcgcgctt cttcaagaaa
     2941 gaactgccgc agccggcgtg cggcgagaat ctcgtcgccg tctttcatgc agcgcgtctg
     3001 aaatgttcca ccctcgtcgt cctcgtaata gaggatgcga ggctcgccgc tcatcaattt
     3061 ccggaaagct tcctttccgg taagaggaat gatgttgcgg tcccaggtaa ggcgcatctc
     3121 gccgaacccc gcgccgttga tccggtactc ggccttgacg ccgggcacat ccttcaactg
     3181 atcggccatg aaacgggctt tcctgttcca cgtttccatc accgtttcgt gatcgagatc
     3241 gacgtagcgg ttcagcgcgg caatcaggcc tacgatctct tctttcccca ccttcattcc
     3301 tcggccgatg tttgcattgg gggaatggtt caacgcggcg gcctcgatga gatctttacg
     3361 gcccgcgaga atacccgtcg attgagggcc gagcaatcct ttgcctccac tgatgacagt
     3421 gaggtccgcg cccatcgtag tatagtgcgt caggctggat accggcggca attccgacgc
     3481 ggcgtcgatg agcacgggta cgccggcttt cctgccgatg ccgatcagtt cttcgagtgg
     3541 gatgatatcg gggcgccgcg agcgttccgt actgcccaac cccgcgagca tcgccgtttt
     3601 cccgccgatg gcgttcgtaa gctgatctct attctccacc tcgacgatcg tcatgccggc
     3661 ggcgcggtac gcgcggtcgt aaaccacccg gtggcctttc ggaatcaggc attctttccg
     3721 gtcccatgcc gggtgcggca gcgccgcaat cttctcccgg tcggtgccgg tgaggcaggc
     3781 ggccgcaccc agcagcatcg cggaaaagga accggccgtc accagagcgg cctcggtctt
     3841 cattacttct gcaatccggc gaccggccgc tgcattcagt tcatacatgt cgacgaactg
     3901 atcgtttgcc tcctccatgg cccgcaggac ttcctggggc atcagggagc cgccataaat
     3961 ggtcgtgtgt ccccggcagg agagcagcgg acgcacgccg agcaggcgcg tgtaaatact
     4021 ttcgtgatac agaggtttgg actccgcgcg tgtaagcggc gccagtgccg ccaccgcgcc
     4081 ggttccgaac aggtcccgcc ggctgagcag gcgtttgagg aagttcgtca ttgcatcgcc
     4141 tcccgattgg agtgagtata ccggaagcgt acaccgtctg gccgccgtac catatcttta
     4201 tgtggaggct tgccggttcc atcatccttc tttgttccac ctccgccctc ttgcgggcag
     4261 aggttcgaaa gctgaccctg aaacagaccg tcgggatcgc gatgaaacag aatcccgata
     4321 tcgtgagcgc gcgactcgac gagcagcagg cggaacaggc tgttcgaatt gcgaaagacc
     4381 ctttcattcc gaaagtcttt gtaggtagcg gggccgccta cagctacggc tatccgatga
     4441 gcatcgaagg ctcggcgcca tcggtgtttc aggcgcgggc tgttgcgtcc atctttaacc
     4501 gcccgctgag ttaccaggtg gcgcaagcga aagaagaagc gcgcggccgc accctggatg
     4561 cggccagccg cagtgaagag gtcgccatgc gtaccacttc gctgttcctc gatgcagaga
     4621 gggcggctcg tgcggcggat atcgcccgtc gacaggtgga aagtgcgcgc aaggtggccg
     4681 aagagaccgc cgcgcgtgtc aaggagggcc gggagctcgc gatcgaatcg cgccgggccg
     4741 aattgaacgt tgcggtggcc cggcagcacg cacaggagct tcaggggcag caggagtatg
     4801 cgcaacgctc actggccatg gttttgggct ttgaccctca ggaccaggtc gagcccgccg
     4861 cagacgagag ggccctgccg gaaccgcctg gttcggaaga atccgcggcg gaaatcgcgc
     4921 tcaggaataa ccgcgaggtc aagcggctcg aatcctctct gctcgccaag ggctatgaag
     4981 tccgggctaa caaggccgca tggctgccga ccgtggacct ggtggcgcag tacggcctct
     5041 tctcaaaatt taacaactac gaggatttct tccggaagtt tcagcggcac aatggacaga
     5101 tcggagtttc cttccaggta cccatttatg ccggcgcggc ttcctcagcg cggaccgcac
     5161 gagcggagtt ggaagtctcg cgcatacggg tggaggtgaa ccgggcccgc ggccgtattg
     5221 cgctggaggc acggcaatcg tatcagcagc tcgaaaacgc gaaaacggcc gccgaagtgg
     5281 cacgtctcga cctggaagtc gcgcgagagc agttgtcgat cctgctggcc cgcatgcagg
     5341 aaggccgcgt atcgatgcgg gaggtggaaa gtgcgcgcta cacggaaaac gaaaagtgga
     5401 tcgcttttct caacgcccag tacgccgtgg agcgggccgc ttacgaactc gcctggcgga
     5461 cgggcagcct gatgggacta ctcctgtaat attgcgggga gcggtaccag cgtactgggt
     5521 ccgcataacc cgcagtccat tgcgcgcctg tggccggtat ggtgaagtag agggagaacg
     5581 gcaaaggaag aatcgatgat gtctcggaag ctgttgttgt gcttgacctt actgttagct
     5641 ttcacggcgt gcaatcgcga ccccaaggtc gtgagcaaga agtatgtgga tagcggcaac
     5701 aagtactttg agaagggcaa gtataaagaa gcggccctga tgtatcgcaa tgccctgaag
     5761 aaggacggtc tgaacggtga ggcatactat cgcctggggt tgaccggcct gcggatgagc
     5821 cggcccggcg aagcttcacg agccctgcgc cgtgctgtgg aattgcagcc ggccaacgtg
     5881 gatgcggcgg cgaggctggc ggacatctat ttagcggcgt acctagctca accgcagcat
     5941 ccgagggaat tgctggagga aacgcagcag attgccgatc gcctgctgaa gcgggatccc
     6001 aaattgtttg agggtttgcg gctggcgggc tacctggggc tcgccaggca ggacctgcca
     6061 aaggctctcg agtatttccg gcaagcgcac gaagtcaaac cgtaccaacc tgacgtctcc
     6121 ctggccatga tgcagacgct gagcgccagc aaccagtttg acgaagccga gaagctggcc
     6181 aaaacggcga tcgaaaagaa caaggagtat cctcagctct acgacggcct ctatatggaa
     6241 tacctgcgcc ggaaccggca ggcggaggcc gaggaaatcc tgaagctcaa gattcggaac
     6301 aatcccacgt cgacggaatc ccgtgtgcag ctagccggct tttacgcggc tacgaaccgg
     6361 cg
//
LOCUS       DASTMK010000130         6442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_615124, whole genome shotgun sequence.
ACCESSION   DASTMK010000130 DASTMK010000000
VERSION     DASTMK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615124"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VFQ79_05325"
     CDS             complement(<1..128)
                     /locus_tag="VFQ79_05325"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU0139105.1"
                     /translation="MRGRVQGVGFRDFAQRAASDLSLNGYVKNLDDGRVEVYAAGPR"
     gene            329..826
                     /locus_tag="VFQ79_05330"
     CDS             329..826
                     /locus_tag="VFQ79_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139106.1"
                     /translation="MKLLRAATCMTVIFALGCAARAQDEKKNEARRLESVTWNSMEHK
                     LTWVIATGEKQGKQPFKSISQQTYTIQMDKAVMTFEGQQRKFSPQEAVTVHRILDLIS
                     KYAVDSTVWWDAGQGVPLDKDGEPRPDLKTDPEIPKAEPVKHIRPGNRLALLELLPAS
                     FQDAQ"
     gene            867..1838
                     /gene="hemB"
                     /locus_tag="VFQ79_05335"
     CDS             867..1838
                     /gene="hemB"
                     /locus_tag="VFQ79_05335"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU0139107.1"
                     /translation="MPFPTHRMRRLRRTESLRSLVRETGLEPRNFILPLFVCPGEGVR
                     REIGAMPGNFQMSVDQIVAECSEAAGLSIGGVLLFGIPETKDETASGAYDDDGIVQRA
                     IRAIKSSVPKLLVLTDVCNCEYTSHGHCGKIAGDDVDNDATLEWLAATALSHAKAGAD
                     IVAPSDMMDGRVAAIRKALDGAGYLNTPILAYAAKFASVFYGPFREAAESAPQFGDRR
                     SYQMDPPNAREALREMELDLEEGADMLMVKPAMPYLDIVRLARDRFDVPLAAYQVSGE
                     FSMIMAAARNGWLDEERAMMESLTCIKRAGAGIILTYFAKQAARLLA"
     gene            1949..3679
                     /gene="polX"
                     /locus_tag="VFQ79_05340"
     CDS             1949..3679
                     /gene="polX"
                     /locus_tag="VFQ79_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEU0139108.1"
                     /translation="MENREFARLLAETGDLMEIAGEDSFRIRSYRIAAGVIEGYPERI
                     ADILADPDRKVTDIPGIGKGIGAILHEIAARGSFERRDELLKKYPATALELLKIQGLG
                     PKAVALLYEHLHVATIDDLERVCKEQKLRTLPRMGARLEEKVLKAICQYRESAGRYLL
                     NYAEKMAEDVSTYLRAGDGVERIAAAGSFRRGRETVGDLDLLVTGPDAEAVLDRFAVY
                     PRVYSVLGKGTNKVSAKVGLDGMQVDVRALPAESYGAALQYFTGSKDHNVALRTRALK
                     MGLTLNEYGLFQLEDNRRVAGETEEQVYAALGLAWIPPELRENQGEIEAAVEGRLPRL
                     LEQGDIRGDLHMHTRETDGRATLEEMAEAAKALGYEYIAVTDHSKSLAMANGLDETRA
                     VAFAQRVRDINQRGELGIHVFSGLECDILRDGTMDLSHDALAELDLVIASVHSHMNLD
                     PAEMTDRLMKALECPHVRILGHPTGRILLHRDPFPFDFERVAAEAARRVVAMEINSSP
                     ERLDLNGTNARIAKSKGCRFILSTDAHHPRHLDNMRYGLVTARRGWLEAGDVLNTLGL
                     EQFSALLRHS"
     gene            3686..5059
                     /locus_tag="VFQ79_05345"
     CDS             3686..5059
                     /locus_tag="VFQ79_05345"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0139109.1"
                     /translation="MQWLKLAALTLIAIAVPAADLRIPVEAYKLDNGMRIILSRDTSA
                     PVVSVYMLYGVGARSETKGRTGFAHLFEHMMFQGSKNAPKGTMDEMIASAGGYLNGST
                     HYDYTDYYEVMPANKLPVALWLEADRMRGLDITAENLANQKEAVKQERRMRLDNQPYA
                     TAIVDLWHEVAFENWANSHSIIGSFEDLNAAGVEDVAKFFKTYYAPNNAILAIAGDLD
                     IPATKKLIQTYFGDIDSQPQPKHPDLSEPESGGAKHEVHKDPLARVPGIIIGYPGPKR
                     RSADYYALVMLDAVLTGGDSSRFQQNLVKGRQSVVQYETNLGWPFASYTDYQDPGVFG
                     MFLLHKPDVTGKQILSQVEEELEKIRKEGVPAKELERAGAFLRSYRLRELQSSLDRAK
                     LLAQHQLLDGDAGYINTEMDRFLAVTPEQIRNVANKYFKTEVRRALEIVPAPEKPAAN
                     TGNTEEK"
     gene            5059..6438
                     /locus_tag="VFQ79_05350"
     CDS             5059..6438
                     /locus_tag="VFQ79_05350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0139110.1"
                     /translation="MKPWILALLAAAAGAQTVDLTKPPETPPLADYKLPPVTLTRAAN
                     GLRIFVVEDKRFPIVTVRWGFQAGSKFDPGDLSGLAETTAALLKEGTTTRTSREIAEE
                     LATIGGAISASTTPDHVILAASALAEHLPKLMEILGDVVLNPSFPEEELELYKQNRVQ
                     ELLAARSQADTLADERFNQVIYGKHPYARILPAGDSVARITRKDVMAFYSKFFALNNS
                     VLIAVGSIPGKIPLPPAYSDWKRKAAPSPPAVAIPAPERRLVLVDRPGSVQADVRVGQ
                     VAVKRSSPEYFPLLVANSMLGGGMNSRMFREIREKKGYAYDAHSEFVPRLEAGSLAAV
                     TQVRNEVAQPALADLLAIMKDLGNTPAPAAELATAKNLLSGTFVLSIETQQGLADRLA
                     SVKLYGLPDDYLDKYVTRIRSVEPAQVRSVASKYIDAGDSALVVVGDASKIAKDLEKL
                     GKFTVEKPQ"
BASE COUNT         1421 a         1877 c         1949 g         1195 t
ORIGIN      
        1 cgcggaccgg cggcgtaaac ttccaccctg ccgtcgtcca gattctttac atagccatta
       61 aggctgagat cgctggcggc acgctgagcg aagtcccgga agccaacgcc ctgcacccgg
      121 cctcgcacgt aatactttcg ggccgattcc gcctccggct ttctggttcc ccgcacgttc
      181 tgatattgta attccaagac gcactcaggg aaaaaaatgc acggctcttc ggatgtcagg
      241 cgggatttgc agaattttga acatggcctc caacttgcat catcaaaatg gaaaggactt
      301 ctctgtgagg tcttttgaag gacgaataat gaagcttttg cgtgctgcca catgtatgac
      361 ggtgattttc gccctgggat gcgcggcgag ggcacaggac gagaagaaaa acgaagccag
      421 acggttggaa tcggtaacct ggaactccat ggagcataaa ctgacctggg tgattgcgac
      481 aggggagaag cagggcaagc agcctttcaa gtccatttcg caacaaacct acacgatcca
      541 aatggacaag gcggtcatga cttttgaggg ccagcagcgt aagttcagtc cgcaggaagc
      601 agtgactgtc catcggattc tcgacctgat ctcgaagtac gccgtggatt ccacggtttg
      661 gtgggatgcc ggacagggag ttccgctcga taaagacggt gaacccaggc ccgatctcaa
      721 aacagacccc gagataccca aggccgagcc ggtgaagcat attcgtcccg gtaatcgtct
      781 ggcgttgctc gagttgcttc ccgcttcttt tcaggacgcg cagtagcagc ggttgagagg
      841 gaagcggcgc gctacaatcc agttgtatgc ccttccccac gcacaggatg cgtagactgc
      901 ggaggacgga atccctccgg agtctcgtac gtgagaccgg cctcgaaccc cggaatttca
      961 ttcttccgtt gtttgtgtgt cccggcgaag gtgtgcgccg tgaaatcgga gccatgccgg
     1021 gcaacttcca gatgtcagtg gaccagattg tcgcggagtg ttccgaagcg gcagggctca
     1081 gcatcggcgg tgtactcctt ttcgggatcc cggaaaccaa ggacgaaacc gcttcgggcg
     1141 cctacgacga cgacggcatc gtacagcgtg cgatccgcgc catcaaaagc agcgtgccga
     1201 agctgctggt gctgaccgat gtgtgcaact gtgagtacac cagccacggc cactgcggaa
     1261 agatcgctgg cgacgacgtc gacaacgatg cgacgctcga atggctggct gctacggcgt
     1321 tatcacacgc caaagcggga gccgacatcg tagcaccctc ggacatgatg gacggccggg
     1381 tcgcggccat tcgcaaggcg ctggatggcg ccggctacct gaacaccccc atcctggctt
     1441 acgccgctaa attcgcctcg gtgttctatg gccctttccg cgaagcggcg gaatcggcgc
     1501 cccaattcgg cgaccgccgc agttaccaga tggatccacc caacgcgcgg gaggcgcttc
     1561 gagaaatgga attggatctc gaagagggcg ccgacatgct gatggtaaag cccgcgatgc
     1621 cttatctcga tatcgtgcgc ctcgcccgcg accgttttga cgtgccgctg gcggcctacc
     1681 aggtaagcgg tgaattcagc atgattatgg cggcggcgcg caacggatgg ctcgatgaag
     1741 agcgcgcgat gatggaatcc ctgacctgca tcaaacgcgc aggcgcgggc atcattctta
     1801 cttatttcgc aaaacaggct gcacggctgt tagcttagta catggtttca ggaccgctcc
     1861 ctcacggtca cggctccgaa cagagccgcg cgcgccagca agcggtaccg gatagcacct
     1921 taattccgaa tagaagcagt tagaccacat ggaaaatcgc gagttcgccc gactgctcgc
     1981 cgagaccggc gacttgatgg agatcgccgg agaagactcc tttcgaattc gcagctaccg
     2041 cattgcagcg ggggtcatcg aaggctatcc cgaacggatc gcagacatcc tcgcggatcc
     2101 ggatcgtaaa gtcaccgata ttcccggcat cggcaaaggc attggggcga ttttgcatga
     2161 gatcgccgcg cgcggttcct tcgaacggcg tgacgaactg ctgaaaaaat accccgccac
     2221 cgcgctcgaa ttactgaaaa ttcagggact cggccccaag gcggttgcgc tgctgtacga
     2281 gcatctgcat gttgcgacga tcgacgacct ggagcgcgtc tgcaaagagc aaaagctgcg
     2341 cacgctgccc cgcatgggcg ccaggctcga agaaaaagtg cttaaagcca tctgccagta
     2401 ccgcgaaagc gcgggccggt acctgctcaa ttacgcggag aagatggcgg aggatgtcag
     2461 tacgtatctt cgcgcgggag acggcgtgga gagaatcgcc gcggcaggca gtttccgaag
     2521 gggccgggaa acggtgggcg acctcgatct gctcgtcacc ggcccggacg cggaagctgt
     2581 cctcgatcgc ttcgccgtgt atcccagagt atacagtgtg ttaggaaagg gcacgaacaa
     2641 agtgagtgcc aaggtcggcc tggacggcat gcaggtggac gtccgggcgc tgcccgccga
     2701 aagctacggc gccgctctgc aatacttcac cgggagcaaa gatcacaacg tcgcgcttcg
     2761 cacgcgtgcg ctgaagatgg ggctgactct gaacgaatac ggcctgttcc agttggaaga
     2821 caaccggaga gttgcgggag aaaccgagga gcaggtctat gccgctctgg ggctcgcctg
     2881 gattccgccc gagctccgtg aaaaccaggg tgagatcgag gccgccgtgg aaggacggct
     2941 tccccggctg ttggaacagg gggacattcg cggcgatctg cacatgcata cgcgcgagac
     3001 tgacgggcgc gccactttgg aagagatggc cgaggcggca aaagctctgg ggtacgagta
     3061 catcgccgtt acggaccact cgaagtcgct ggccatggcg aacgggctcg atgaaacccg
     3121 cgccgtcgct tttgcacaaa gggtccgtga catcaaccaa aggggcgagc ttggaatcca
     3181 cgttttctcg gggctggagt gcgacatcct gcgggatggc accatggatc tgtctcacga
     3241 tgctttggcc gaactggacc tggtgatagc cagcgtgcac agtcacatga atctggatcc
     3301 cgccgagatg acagaccgcc tgatgaaggc cctggagtgc ccgcacgtgc gcatcctggg
     3361 ccaccctacc ggccgcatcc ttctgcaccg cgatccgttc cccttcgatt ttgaacgtgt
     3421 agcggctgag gccgcgcgca gggtcgttgc catggaaatc aattccagcc cggagcggct
     3481 cgacctgaac ggcaccaacg cacgcatcgc aaaatcgaag ggatgccgct tcatcctgtc
     3541 cacggatgcg catcacccca ggcatctcga caacatgcgc tacggattag taacggcccg
     3601 ccgtggatgg ctggaggcgg gagacgtgct caacacattg ggcttggaac aattctcagc
     3661 tttactccgt catagttaaa aacttatgca gtggcttaaa cttgctgcgc taaccttgat
     3721 cgcaatcgcc gtgccggcgg cagacctccg gatacccgtc gaggcctaca agctcgacaa
     3781 cggaatgcga atcatcctgt cacgggacac ctccgctccg gtggtaagcg tttacatgct
     3841 gtacggcgtg ggagcgcggt cggaaaccaa gggccgcacc ggctttgcgc acctctttga
     3901 acacatgatg tttcagggtt cgaagaacgc gccgaaaggc accatggacg agatgatcgc
     3961 ctcggcgggc ggctatttga acggctccac gcattatgat tacaccgact attacgaggt
     4021 gatgcccgcg aacaaacttc ccgtcgcgct atggctggag gcggaccgga tgcggggcct
     4081 ggatatcacc gcggaaaacc tggcgaatca gaaggaagcg gtcaagcagg agcgccggat
     4141 gaggctggac aaccagccgt atgccacggc catcgtggac ttgtggcacg aggtggcgtt
     4201 tgagaattgg gcgaattcac actcaattat cggctctttt gaagacctga acgccgccgg
     4261 tgtcgaggac gttgcgaagt ttttcaaaac ttactacgcc ccgaacaacg ccattctggc
     4321 cattgcgggc gatctggaca tccccgcgac gaagaaactg attcagacgt attttggaga
     4381 catcgattcg cagccgcagc cgaaacatcc cgatctttca gaacccgaga gcgggggcgc
     4441 aaagcacgag gttcataagg atcccctggc aagggtgccc ggcatcatca tcggctatcc
     4501 cggacctaaa cgccggtcgg cggattacta cgcgctggtc atgctcgacg ccgtcctcac
     4561 cggcggcgac agctcgcgct tccagcagaa cctggtgaaa ggccgacagt ccgtcgtgca
     4621 atacgaaacg aatctcggtt ggccttttgc ttcgtacacg gattatcagg atccgggcgt
     4681 cttcggaatg tttctgctgc ataagccgga cgtaacgggc aaacagattc tttcccaggt
     4741 ggaggaggag ctcgaaaaga tccgtaaaga gggcgtgccg gcaaaggaac tggaacgcgc
     4801 cggagccttt ctgcgttcct accgcctcag ggagttgcaa tcgtcgctgg accgggcgaa
     4861 attgctggca cagcatcaac tgctggatgg ggatgccggt tatatcaaca cggaaatgga
     4921 ccggttcctc gccgtgacgc cggagcagat ccggaacgta gcaaacaagt acttcaagac
     4981 agaggtgcgg cgcgcgctgg agatcgtccc cgcgcccgaa aagccggcgg cgaacaccgg
     5041 caatacggag gagaagtgat gaagccctgg atactggcgc tcctggccgc tgccgctggc
     5101 gcacaaacgg tggatttgac caaaccgccc gagacacctc cgctggccga ttacaaactg
     5161 ccgcccgtaa cgctcacgcg ggccgccaat ggcctgcgga ttttcgtcgt cgaggacaag
     5221 cggtttccca tcgtaacggt gcgctgggga tttcaggccg gctctaagtt cgatcccggg
     5281 gacctgagcg gattggcgga aactacggcg gcgctgctca aagaaggcac caccacgcga
     5341 acttcgcgcg agatcgccga agagcttgca accatcggag gagccatcag cgcgagcacc
     5401 actcccgatc acgtcatcct cgcggcctcc gcgctcgcgg aacacctgcc caagctgatg
     5461 gagatcctgg gcgacgtcgt cctgaacccc agctttccgg aagaggagct ggagctttac
     5521 aaacagaatc gcgtgcagga actgctggcg gcgcgttccc aagccgacac gctggccgac
     5581 gagcgcttca accaggtcat ttacggaaag catccttacg cgcggatact gcccgcgggc
     5641 gattcggtag cgcgaatcac acgcaaagac gtgatggcgt tctattcgaa gtttttcgcc
     5701 ctgaacaaca gcgtgctgat cgccgtaggt tcaatacccg ggaagattcc ccttccccct
     5761 gcgtactccg attggaaacg caaagcggcc ccctcgccac cggcggtggc catcccggct
     5821 cctgaacgga ggttagttct ggtcgaccgg ccaggttcgg ttcaggcgga cgttcgcgtc
     5881 gggcaggtag cggttaagcg gtccagcccg gagtactttc cattgctggt ggcgaattca
     5941 atgctcggcg gtggaatgaa ctcccgcatg tttcgcgaaa ttcgggaaaa gaagggctac
     6001 gcttacgatg cccactccga gtttgtgccc cgcctggaag ccggatcgct ggccgccgtt
     6061 acgcaggtcc gcaatgaagt cgcacaaccc gcattggcgg atctgctggc catcatgaaa
     6121 gatttgggca acaccccggc acccgccgct gagcttgcaa ctgccaagaa tctcctgagc
     6181 ggaaccttcg ttctgagcat cgagacccag caaggccttg ccgaccggct ggcctccgtg
     6241 aaactgtatg ggctgccgga cgactacctg gacaagtatg tgacgcgcat acggagcgtc
     6301 gagccggcgc aggtgcgctc ggtggcgtcg aaatatatcg atgcagggga ctccgctctg
     6361 gtggtggtcg gcgacgcctc gaaaatagcc aaggatctcg aaaaactcgg taaattcacg
     6421 gtggaaaaac cgcaataatg aa
//
LOCUS       DASTMK010000131         5672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_390499, whole genome shotgun sequence.
ACCESSION   DASTMK010000131 DASTMK010000000
VERSION     DASTMK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390499"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VFQ79_05355"
     CDS             <1..1285
                     /locus_tag="VFQ79_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0139111.1"
                     /translation="DGIQQACEMARIRVIVTSRTFLEKARLQEMVQKLQRVQVVYLED
                     LRKTFGVLDKLWLMAWALWFPRAAMSKVRPEEPAAILFTSGSEARPKGVVLSHGSILA
                     NIEQIKAIIEFAPSDKFMAALPLFHAFGLTAGMLLPLLNGSPVFIYPSPLHYRRIPET
                     AYEYDCTVLFGTNTFLANYAKVAHPYDFARMRYVVEGAEKLTAEVRQIYMEKFGVRIL
                     EGYGATECSPVLAVNTPFAYRTGSVGELLPRIEARLESVEGIEEGGILHVRGPNVMLG
                     YLKEGHPDGIEPPSSIYGPGWYNTGDIVSIDADGLVHIIGRVKRFAKVAGEMVSLEMV
                     ERIAGAASPGVQHAAVAVPGTTRGETVVLFTEDAGLKREQLQDAARRLGAPDLALPRH
                     VLYAERLPLLASGKRDYPAINRMAKDRTAIAGRGR"
     gene            1287..2609
                     /locus_tag="VFQ79_05360"
     CDS             1287..2609
                     /locus_tag="VFQ79_05360"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0139112.1"
                     /translation="MLPELNRKREQRAWYVYDWANSAFSTTVVTLFLGPYLTALARAA
                     ADSNLRIHPLGISIDARAYWSYLVALSVALQVVVLPMAGAIADHSRRKKLLLGLFAYA
                     GALCTMALYFVAGDRYGLGGALFVVANVAFGASIVIYNSYLPEIATPEERDRVSSKGW
                     GMGYLGGGLLLALNLLLYQNAAAWGLSESMAVRISLCSAGVWWAIFSIMPLRVLRNHG
                     PIRSPAGGVRSPLSDGFRQLLKTLRGLRHYPQTLLFLLAYLLYNDAIQAVINLSTQFG
                     NDELGISISSLTMVILMVQFVAFFGAIGFGWFAQGVGAKPAVMVALVIWTAVLVAMYL
                     WVRTTAQFFVMAGVVGLVLGGSQALSRSLFSLMIPKGKEAEYFSLYEISDKGTSWMAP
                     LIFGLALQFTASYRLAILSLIVFFVLGLVLLWKVDVARAAREAAGRDR"
     gene            2894..4789
                     /locus_tag="VFQ79_05365"
     CDS             2894..4789
                     /locus_tag="VFQ79_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0139113.1"
                     /translation="MKSLISVEDTYAVLFERNPHPMMVYDPSGGEIVAVNEAAVSTYG
                     YSREQFLRMNIRDISAPEELPRLEAHISRQRPSYEYSGVWRHLRRNGSIIAVEVNSSE
                     LPYNGRPARLISVNDVSRSVLAEEALRQSDANFRTLIQSAPLAIVTLDRNGLVQMWNP
                     AAEHLFGWREAEVLNRPLLTVPENDIDEHRLLLARAAGGDPVVGIERTRRRKDGTGVL
                     ISISVAALRDDRGGIAGFIATYMEVGTRRALEEQFRHAQKMESVGRLAGGVAHDFNNL
                     LTVILGYGHMLRAMDTLDASVVSGIGEIVDAAERAADLTRQLLAFSRKQVLQPEILTL
                     DRIVRRMGRLLKRLIGEDIELVTVIPQSTHPVRVDPAQIEQVVANLVVNSRDAMPGGG
                     RLTIEISNVELDETYASRRVDFQPGKYVMLAVTDTGTGMDQAVQERIFEPFFTTKEPG
                     KGTGLGLATVYGIVKQSGGDVWAYSEPGTGTTFKVYLPAVITTAPPEAEAPAVIPAVG
                     GSETILVVEDEEKVRMLAAGVLKAAGYHVFQARSAGEALFILRQRPDEIQLLLTDAVL
                     PMMSGEQLAKRAVEIRPGIRLLFMSGYTDTSVVANGVIAEGNVFLQKPFTPEILLRKT
                     REALERQ"
     gene            complement(4810..>5672)
                     /locus_tag="VFQ79_05370"
     CDS             complement(4810..>5672)
                     /locus_tag="VFQ79_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU0139114.1"
                     /translation="FLDPLGSPDTVNAIARTGATAFSMELIPRITRAQSMDALSSMAT
                     VAGYKAVLLAADRLPKLFPMLMTAAGTVTPAKVFVIGAGVAGLQAIATARRLGAVVQA
                     YDVRPAVRDQVESVGAKFVELPLETSDTEGKGGYAKAQDEAFYTKQRELMGRVVADSD
                     VVITTAAVPGKTAPVLVTKEMVAGMASGSVIVDLAAERGGNCELTVPGQTVTEHGVSI
                     LGPLNLASAVPYHASQLYAKNIATFLLHIAKGGTINLDGDDEILRETLVARDGQVVNS
                     RVRELMEAKS"
BASE COUNT         1105 a         1574 c         1785 g         1208 t
ORIGIN      
        1 cgacggcatc cagcaggcct gcgaaatggc tcgcattcgg gtgattgtca cgtcgcgaac
       61 gttccttgaa aaagcacggt tgcaggagat ggtgcagaag ctgcagcgag tgcaggtcgt
      121 ctatctcgag gatttgcgca agaccttcgg tgtgttggac aagctctggc tgatggcctg
      181 ggccctatgg tttccgcggg ccgcaatgtc gaaggtgaga cctgaagagc cagccgctat
      241 cctctttacc tccggctcag aggcccggcc gaagggtgtt gttctgagcc atggctcgat
      301 tctcgcgaac atcgaacaga tcaaagcgat catcgaattc gctccttcgg ataaattcat
      361 ggccgcgctc ccgctgttcc atgcgttcgg gttgacggct ggcatgttac tgccgctgct
      421 gaacggctcg ccggttttca tctatccttc gccgcttcat tacaggagga tcccggagac
      481 ggcgtacgag tacgactgca cggtcctttt cggaacgaat acatttcttg cgaactacgc
      541 gaaggtggcg cacccatacg atttcgcccg gatgcggtac gtggtggagg gagccgagaa
      601 gctgaccgcc gaagtgcggc aaatctacat ggaaaaattc ggcgtccgca ttctggaagg
      661 gtatggcgca acggaatgtt cgccggttct ggcggtgaac acgccgtttg cttaccggac
      721 gggcagcgtc ggcgaactac taccgcgcat cgaggcccgt ctcgaaagtg tagagggaat
      781 cgaggagggc ggcattctcc atgtccgcgg acccaatgtc atgctcggat acctgaaaga
      841 agggcatccg gacgggatag agcctccttc ttcgatttac ggacccggat ggtacaacac
      901 gggggacatt gtttcgattg atgcggatgg gctggtgcac attatcggac gggtgaagag
      961 attcgcgaaa gtggccggag agatggtatc gctcgagatg gtggagcgga ttgccggggc
     1021 cgcatcaccc ggagtacaac atgccgccgt ggccgtgccc ggaaccacac gtggtgaaac
     1081 ggtcgttctt tttacggagg acgcgggact gaaacgcgaa cagttgcagg acgctgcacg
     1141 ccggcttggc gcgcccgacc tggcgctgcc gcgccatgtc ctgtatgcgg agcggctgcc
     1201 gctgctggcg agcggaaaac gggactatcc ggccatcaac cggatggcga aagatcgaac
     1261 cgccatcgcg ggccgaggcc gttagcttgc tcccggagct taaccgcaag cgtgaacagc
     1321 gcgcctggta cgtctacgac tgggcgaact ctgcgttctc caccaccgta gtgacgcttt
     1381 tcctgggacc gtacctgacg gccctggcga gggcggcggc cgattcgaac ctgcgcattc
     1441 atccgctggg tatctcgatc gacgcgcgcg cctactggag ttacctcgtt gcgctctcag
     1501 tagcgctcca ggtagttgtg cttccgatgg ccggagccat tgccgatcac agccggcgca
     1561 agaaactgct gctcggcctg tttgcttatg ccggagctct ttgcaccatg gccctgtatt
     1621 ttgtcgcggg cgaccggtac gggttgggcg gtgcattatt cgttgtcgcg aacgtggcgt
     1681 ttggcgcctc catcgtcatc tacaactcgt atctgccgga gatcgccacg cccgaggagc
     1741 gcgacagggt ctcttcgaaa ggttggggca tgggctacct cggagggggc ctgttgctcg
     1801 cgctgaatct gctgctgtat cagaacgccg ccgcttgggg cctgtcggaa tccatggcgg
     1861 tacgcatcag tctctgctcc gccggggtgt ggtgggcgat attctcgatc atgccgctca
     1921 gagtactacg gaatcatggc ccgatccgga gcccggccgg cggcgttcgg agcccgttat
     1981 ccgacggctt ccggcaactt ttgaaaaccc tgcgaggatt acggcattac cctcagacgc
     2041 tgctgttcct gttggcatac ctgctctata acgacgcgat tcaggccgtg atcaacctgt
     2101 cgacgcagtt cggaaacgac gaactcggaa tctcaatttc tagtctcaca atggtgattc
     2161 tgatggttca gttcgtggcc ttcttcggcg caatcggatt cggatggttt gcccagggag
     2221 tgggtgcgaa gccggcggtc atggtcgcgc tggtgatctg gacggcggtt ctggttgcga
     2281 tgtatctttg ggtccgcacc actgcccagt ttttcgttat ggctggagtg gtgggcctcg
     2341 ttctgggcgg cagccaggcc ctgagccgct cgctgttttc tctcatgatc ccaaaaggaa
     2401 aagaggcgga atatttcagc ctgtacgaga tcagcgacaa agggaccagt tggatggctc
     2461 ccctgatttt cgggcttgcg ctacagttca ccgccagcta ccgtttggcg atcctgtcgc
     2521 tgatcgtgtt ctttgtgctg gggctggtgc tgctgtggaa agtggacgtc gcacgagcgg
     2581 caagggaggc tgcagggaga gatcgctaag caggaggact acactggcgg attccgggta
     2641 taaagcagaa tatgaattgc gtggaacccg ccagcctgta atgaatccgg gaattagtcc
     2701 gggaaacttc ctaaacttga ttttagaggt tccagtctga aaagagaaga actccattgc
     2761 gggtatccaa ctgttttcac gtgggtactg aatccacccc agctagggaa atagactgag
     2821 aactttctgt aatcacacac tactctctta agcgaggtgt cttaggtcac tggttgaatt
     2881 cttatctata ttcatgaaaa gcttgatatc ggtggaagac acatatgcgg tgctattcga
     2941 gcgtaacccc cacccgatga tggtgtatga cccctccggg ggggagatag tcgcggtgaa
     3001 cgaagctgcg gtatctacgt acgggtactc gcgcgaacag ttcctccgga tgaatatccg
     3061 cgatatcagt gcgccggaag agctgccccg gctcgaggct catatttcgc gccagcggcc
     3121 cagttacgaa tacagcggtg tgtggcgcca cctgcgccgg aatggcagca tcatcgccgt
     3181 ggaggtgaac tcctccgagc tgccgtataa tggccgtccg gcgcgcctca tttccgtcaa
     3241 cgatgtttcc cgtagtgttc tggcggagga ggcacttcga cagagcgacg caaactttcg
     3301 cactctgatc caatcggctc cgctcgctat cgtcaccctg gaccgcaacg gactggttca
     3361 aatgtggaac ccggctgccg agcatctttt cggctggcgg gaagccgaag tcttgaaccg
     3421 tccgcttctc acagtgccgg agaacgatat cgacgaacac cgcttgctac tcgcccgtgc
     3481 ggctggcggc gacccggtgg tgggcataga acggacccgg cgccgtaagg acgggacggg
     3541 tgttctgatc agcatctccg tcgccgctct ccgcgatgac agaggcggta ttgccggatt
     3601 catagcgacc tacatggagg ttggtaccag gcgggctctg gaggagcagt tccggcatgc
     3661 ccagaaaatg gaatcggttg gaaggcttgc cgggggagtg gcgcatgatt ttaataacct
     3721 gctgaccgtc atcctcggtt atggtcacat gctccgcgct atggacacgc tggacgcatc
     3781 cgtcgtatcc ggcatcgggg agattgtgga tgccgcggaa cgggctgccg atcttacgag
     3841 gcagttgctt gctttcagcc gcaagcaggt attgcagccg gaaatcctga cactcgaccg
     3901 cattgtccgg cggatggggc ggctgttaaa gcgcctgatc ggcgaggata ttgaactggt
     3961 gacggtcatc ccccagagca ctcatccggt acgggtggat cccgcgcaaa tcgagcaggt
     4021 ggttgccaac ctggtggtga attcgcgcga tgccatgccg gggggcggcc gtctgacgat
     4081 cgaaatatcg aacgtcgagc tggacgagac ctacgcgagc agacgggtgg attttcagcc
     4141 gggcaaatat gtgatgctgg cggtcaccga caccggaacc ggtatggacc aggccgtaca
     4201 agagcggatt tttgaaccgt ttttcaccac gaaggagccc gggaaaggta cgggacttgg
     4261 actggcgacg gtgtacggca tcgtcaagca gagtggcggg gatgtttggg cctacagcga
     4321 gcctggcact ggaaccacat ttaaggtcta tctgcctgcg gtgataacga cggctccgcc
     4381 cgaggccgag gcgcccgcgg tgattccggc cgtggggggc tctgagacca ttctggtggt
     4441 tgaggacgaa gagaaggtac gcatgttggc ggcgggggtt ctgaaagcgg ccggctacca
     4501 tgttttccag gcgcgcagcg cgggtgaggc gctgttcatc ctgcgccagc gtccggatga
     4561 gatccaattg ttgttaacgg acgcggtgct tcccatgatg agcggtgagc aactggcgaa
     4621 gcgcgcggta gaaatccggc ccggcatccg gctcctcttc atgtcgggat ataccgatac
     4681 ctcagtcgtc gccaacggag tgattgccga aggaaacgtc tttttacaaa agcctttcac
     4741 cccggagatc ctgttaagga aaacacggga agcgctcgaa cgccagtaag ctgcggccca
     4801 ggagttccgt caggattttg cttccatcag ttctctgacc cgcgaattga caacctggcc
     4861 gtcacgagcg accagtgttt cgcgcaggat ctcgtcatcg ccgtcaaggt tgatggttcc
     4921 tcctttggcg atgtgaagaa ggaacgtggc gatatttttc gcatacagtt ggctggcgtg
     4981 atagggaacg gcggaagcaa ggtttaacgg cccgagaata cttacgccgt gctccgtcac
     5041 cgtttgcccg ggcactgtca gctcgcagtt gccgccgcgt tcagccgcga ggtccacaat
     5101 cactgatccg gaagccatac cggcgaccat ctctttcgtg acgagaacgg gtgccgtttt
     5161 gccgggcacc gccgcggtgg taatgacgac atcactgtcg gcgacgacac gtcccatcaa
     5221 ttcgcgctgc ttggtgtaga aagcctcgtc ctgtgccttg gcatagccgc ccttaccttc
     5281 cgtgtccgag gtttcaaggg gaagctcgac gaacttggct cctacacttt ccacctggtc
     5341 gcgcacagcg ggacgtacat cgtaagcctg cacgacagcg ccgagacggc gggccgtcgc
     5401 gatggcctgt agacccgcta ctccggcgcc gatgacgaat accttcgcgg gcgttacggt
     5461 cccggcggct gtcatcagca tgggaaacag cttcggcaac cggtcggctg cgagcaggac
     5521 cgccttgtat ccggcgacgg ttgccatgga ggagagcgca tccatactct gcgcgcgcgt
     5581 aatgcgcggt atcagctcca tgctgaatgc ggtggcgccc gtgcgtgcga tcgcgttcac
     5641 cgtatcgggt gagccaagcg gatccaggaa cc
//
LOCUS       DASTMK010000132        11844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_38890, whole genome shotgun sequence.
ACCESSION   DASTMK010000132 DASTMK010000000
VERSION     DASTMK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11844
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38890"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VFQ79_05375"
     CDS             complement(<1..1265)
                     /locus_tag="VFQ79_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139115.1"
                     /translation="MRWWRTVVEWRLWSSRQRRETDFDRELRDHLDLEAEERQESGIS
                     PDEAQYAARRAFGNVTLVKEDMRGIRTWTSVEILIKDLRYALRTLGANPLFTLTAVLS
                     LALGIGANTAIFTLLYVSVWKPLPVKEPRQIFQVMRSNAHGEFGSSYVLLRELSDAVR
                     SFGEIFAKAAYGVRKFGVDGDSSERVVGEPVSANFFSSLRVNPAIGRMLEPPDDSVLG
                     GNRVAVLSHAFWTRRFQSNPAVLGKTILYEETPYTVVGVAQPGFTGMDPQTPIDIWVP
                     VTADADKAWLTNAYNSWLTFFMRLAPGSEPARIQSVLEGVFRSHLKREVLPGVSTHFR
                     PLLEAQHVTLQPASSGMSITGRRYEKPLMLLMTVVALVLLISCANVANLVMARNSSRQ
                     REINVRLAIGASRRRLVSQLFTEALVIALL"
     gene            complement(1267..1620)
                     /locus_tag="VFQ79_05380"
     CDS             complement(1267..1620)
                     /locus_tag="VFQ79_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139116.1"
                     /translation="MAKESSGRIDLLQGTLDMLILRTLLFGPAHGHQIAKHIQRTTGD
                     VLQVQHGSLYPALHRLERRGWVASKWEMAKDRNREFKYYRLTPAGRKQLTVEESKWKQ
                     LAGAIARVMWSPEEG"
     gene            complement(1851..2015)
                     /locus_tag="VFQ79_05385"
     CDS             complement(1851..2015)
                     /locus_tag="VFQ79_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139117.1"
                     /translation="MRKPGRKRWHEREALEQPLAVHQHWHIDISYINISGTFYYLCSI
                     LDGCSRYIVN"
     gene            2125..2469
                     /locus_tag="VFQ79_05390"
     CDS             2125..2469
                     /locus_tag="VFQ79_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139118.1"
                     /translation="MTGRQKRRDEIPYGTLDMLILKTLSTAGEMHGFQIADYIQENTE
                     SVLNVEEGSLYPALQRLLVHGWVTAAWGRTEGNRRARYYRLTPAGRKQLEREMEQYYR
                     LTHAIGRILQPA"
     gene            2479..5181
                     /locus_tag="VFQ79_05395"
     CDS             2479..5181
                     /locus_tag="VFQ79_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139119.1"
                     /translation="MGIADIWRRILFLVRRTRADEELAEEMRLHMELRAAKLQQQGVE
                     ADDAALTARRRFGNTTAIRETSRDTWGWRWLEDALQDLRFGLRMLARSPGFAAIVVLT
                     LGLGIGASTAIFNVVNGVLLAGLPFQEPDRLVALHERMEKRIDRDFRFSPPDYIVLTQ
                     QKDWFSSVGSFRNIQYEITGSGTPERVTGAKVTASLFETLGVDALMGRTFTDAEDHEG
                     HQVALLSHAYWKRKFGQDASVIGRGIRVDRRLYTIVGVLPPSFVFPPRGMPSNGTPAD
                     VFLPMSFTAGQKQAYGMQYSHSVVARLKPDIGIEQVRAAAPMLARRFEERYPAFFQKI
                     PGFSLSVTIAPFRDKIVGNVQTLLLVILAAVLMVLLIGCADVASLMLARATGRAREMA
                     IRVALGAGRFRLIRQVLVESGVLAFLGGAFGVFLASISTNALMAASPIELPRLEAVVM
                     NQHALLFAAAISMLTALLFGLVPAIESSRGEAADALREGGHGRTPSLRRRRWLRSFVA
                     VQVALAVVLAISAGLLMRSFTKLLEVNPGFRPQQVLGLSLNLPFESYRQAAQVTGFWE
                     RLREHLSTIPGVEAVGIGDLPLAVRESRAIWPEDTSAFESNPPQIRQSWVHGEYFRAL
                     GVPLLEGRWFTDRDGKDSQRVIILNETMARIFYPGKNPIGQRLKWGGSAESPNPWMTV
                     IGVVGDIKQESLQERTAPMTFTPLMQEPDEAIAPPLSMLRAMNVALRTSGDPPAIASA
                     VRKRVAGLDPALPVADMKTMEDAVRQAAAPQRFSTYLLGAFAAIALLLATLGIAGVVA
                     YSVGQRTREISLRIALGASRRTILSLTLREGLLYAGLGLAAGAGLALGLTWLMGSLLY
                     GVQATDAVTFAGVMGVVGVVAAASSLLPGYRATRLDPMVALRNE"
     gene            5226..6533
                     /locus_tag="VFQ79_05400"
     CDS             5226..6533
                     /locus_tag="VFQ79_05400"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0139120.1"
                     /translation="MLRLVLLSLLTLPLAAQDRIATVQVRVSTDRSDWRYEPGQPVRF
                     RIVAVEDGHALSGVKVTYRVGPEMMPPKIERTETLTADGLIIDGGTMKEPGFLRCIAT
                     VEQNGKTYRGLATAAFHPESIQPTQKDPADFDAFWAAGKAALAKLPIDARITPLPEYG
                     NASADCYEVNLQNIGMGNAPSRFYGILCEPKAPGKYPALLAVPGAGVRPYRGLAAMAA
                     RGILTLQVGIHGIPVTLDQSVYDSLGAGALMNYNTFGLDHRDRYYYRRVYLGCVRAND
                     FLTSHPKWDGVNLAVTGGSQGGALSIVTAALDPRVRGLAAYYPALSDVTGYLENRAGG
                     WPHMFRAADGPLSHRSPDKIETSRYYDVVNFARRVKVPGLYSWGFNDQTCPPTSMYSA
                     YNVIPGQKKLLLALETGHNNIPEQVAEVQAWLEAFLKTASAGN"
     gene            6620..7855
                     /locus_tag="VFQ79_05405"
     CDS             6620..7855
                     /locus_tag="VFQ79_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0139121.1"
                     /translation="MNSINLGQLLICTLLGHALAQPPKPAHIIPNDPLFRLQYSFFNP
                     NQNSVRFPAARAGQPDVEMPATPEVDLAVTKAWTLTTGNRSVIVALLDDGFFYQHEDI
                     RDNIWKNPGETGTDATGRPKETNGVDDDGNGFIDDVAGWDFAFDDPDPDAYVFDGMDR
                     TRIQPFWHSISALGIIGAKGNNGTGVAGINWDLSMMLLKIGAQGIRRGETDTNRKTRA
                     ARAIRYAVDNGARVINWSGFVEDKSNLEELQGAFEYAEQHGVLIVVAAGNSGLDLDVP
                     GNAGYPGSFTNSNIIRVAELTFTGGLDRYSGRDRISSSNFGARTVEIAAIGRNYTTDV
                     RNGLSAYRLSGGTSSSAPVVTGVAALVLSVRPDLRAEQIKQILLRSARRLPEIEGKVI
                     ANGIIDAYAAVTTALRWSR"
     gene            8064..9965
                     /locus_tag="VFQ79_05410"
     CDS             8064..9965
                     /locus_tag="VFQ79_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139122.1"
                     /translation="MQRKLTSATTLDNLRREVKRWLKALREDNPEARERFERAYPKQT
                     TTPVLRDVQYALAREYGFENWKELKLAVQQAARSRTREVYERAARDFADAYKGDAAAL
                     DRLNRHYGRSFSLADLKAEIWRRDYAYRQRSFRAPESYFPVEEAQIIVAQDAGFGSWK
                     KLMDALATGVPPQGAPYALDTKDNRIGPRRRMPGTDWDELIGVMKERRIPALDANGFM
                     TDEALARIAGLNFVTSLSLGGSRELTDDGLRHLVRMPQLEHLDLNEYPGGKLTDRGLE
                     VLRHLPNLRKFKMTWQSGVTDRGVANLRFCERLEEVNLMGSPTGDGGIEALQGKAKLR
                     RFSTGRLVTDAGISLLHNFPLLETADGEGARLLVDGPFTNTGLAALAGLEGIFELDLF
                     WHVDRITTDGFAHLVHLPNLMALGCDGKLSDDVALRHIAAMPRLKRLRIQESVATDDG
                     FVALARSRTIEGIWGRVCPNFGSRGFIAFSKMPSLRSLGIGCKNVDDAALALLPEFPT
                     LRELTPIGFQDDGFRHIGRCRNLERLTCMYCRETGDTATEHIRELAIKYYYSGLTQIT
                     DRSLEILGGMESLEQVDLYECLKVTDAGLPFLARLPRLREVHLDSLPGVTLEGTKVFR
                     PGVHVYYST"
     gene            complement(9976..10554)
                     /locus_tag="VFQ79_05415"
     CDS             complement(9976..10554)
                     /locus_tag="VFQ79_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139123.1"
                     /translation="MSCVKPVDAAILADYWLAALPESEEEAIEEHLFACDECGTRLRE
                     MILLANAIRDLARDGSLLMVVSDAFLKCAAEEGLRIREYAPPAGGSVACTVTAEDDFL
                     IGRLTADLSAATRVDLCICDKHGMERFRLADIPFDAAAGSVAFQQSITYAKAASSETM
                     VARLVAFDDSGSERLLGEYTFNHTRTLPGPGA"
     gene            complement(10551..11702)
                     /locus_tag="VFQ79_05420"
     CDS             complement(10551..11702)
                     /locus_tag="VFQ79_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0139124.1"
                     /translation="MAASKKNAARLGAHIVFADESGFLLIPHVVKTWAPRGQTPVHRH
                     CYRRDKISVISGVSVSPKRQRLGLFYQLYFNNIGHDEVCVFLRDLLRHLRGHVIAILD
                     NSQTHHGQPLAELQMRHRRLHIEHFPSYAPELNPDEGVWSLAKRDLANGCPHNVEELM
                     DDLVRSIERIRRSPAKLRGCIKQSKLPLFFALTVALFMHRSIVEIELNEADLVHRIGT
                     GQDPAAEEELFRRVAPRIRLYGLRHLRDEHAAEDLVQQVLVIAIEALRAGRLRDSGKL
                     TSFVLGTCRMTVLDLRRSTLRKERLLEQFGVDMLAPAPTSTPRLDREELRRCIQKLRE
                     RERAVIVMSFYGEETSLEVARFLGVSEANVRVIRHRAIHQLRHCMEAAA"
     gene            complement(11627..>11844)
                     /locus_tag="VFQ79_05425"
     CDS             complement(11627..>11844)
                     /locus_tag="VFQ79_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0139125.1"
                     /translation="IRCEFGVSYHRAHIGRLMHTLNWSHQKPEKRAVERNEEEIERWK
                     QKEWPRVKKTLRGWAPTSSLPTNPDSC"
BASE COUNT         2359 a         3544 c         3603 g         2338 t
ORIGIN      
        1 aggagcgcga tcacgagagc ctccgtaaac aactggctca ccaggcgccg gcggctggcg
       61 ccgatcgcaa gacgtacatt gatctcgcgt tgccggctgg aatttcgagc cataaccaag
      121 ttggcgacgt tggcacacga gatcaacagt accagcgcga ccacagtcat cagcagcatc
      181 agcggttttt cgtaccgccg tccagtaatc gacatgccgg aactggcggg ttgcagcgta
      241 acatgttggg cttcgaggag ggggcgaaag tgcgtcgaca cacccggcag aacttcgcgc
      301 ttcaggtgag accggaacac gccctccaag acgctttgga tccgtgccgg ttcggagccg
      361 ggagcgaggc gcataaagaa agtcagccac gagttatacg cattcgtcag ccatgctttg
      421 tcagcatccg cggtgaccgg tacccaaatg tcaatcggtg tttggggatc catacccgtg
      481 aatccgggtt gtgcgacacc cacaactgta tagggagtct cttcatacag gatcgttttg
      541 ccgagcacag ccgggtttga ctggaagcgg cgtgtccaga atgcatgact gagcacggcg
      601 acacggttcc caccaaggac gctatcatcc ggtggctcga gcatgcgccc aatggccggg
      661 ttcacccgca gggaggaaaa gaagttcgcc gagaccggct cgccaacaac gcgctcactg
      721 gagtcaccat cgacgccgaa cttcctcaca ccataggctg ccttcgcaaa gatctcaccg
      781 aatgagcgca ccgcatcgct gagctctcgc aggagcacat atgaggagcc gaactcgccg
      841 tgcgcgttgc ttctcatgac ctggaagatc tgtcgcggtt ccttcacggg cagaggcttc
      901 cagacagata cgtagagaag cgtgaaaatc gctgtgttgg ctccgatacc gagggcgagg
      961 gaaagcacgg cggtaagcgt gaatagcgga ttcgctccca gcgtgcgaag agcatagcgc
     1021 aggtccttga taagaatttc aaccgatgtc cacgtccgta ttccccgcat gtcctccttc
     1081 actaaagtca cattgccgaa ggcgcgccgt gcagcgtatt gagcttcatc gggagatatg
     1141 cctgactcct gccgctcttc cgcctctagg tccagatgat cgcgcagttc gcggtcaaag
     1201 tctgtttcac gtcgttgccg tgaggaccag agccgccatt ccacaaccgt acgccaccaa
     1261 cgcatttcag ccctcctcgg gcgaccacat cacccgtgcg atcgcaccag ccaattgctt
     1321 ccacttagac tcctccaccg tcaattgttt cctaccggcc ggtgtgagcc ggtagtactt
     1381 gaactctcga ttacggtcct tggccatctc ccacttcgag gccacccaac cccttcgctc
     1441 cagccggtgc agggccgggt agagggagcc gtgctggact tgcaggacat ccccggtggt
     1501 gcgctggatg tgcttggcga tctgatgtcc atgggcagga ccgaacaaca gcgttctcaa
     1561 aatcagcatg tcgagcgtgc cctggagcag gtcgatacgg ccggaagatt cctttgccat
     1621 gtcgctggta gatattctac ctccaaattt atcgttaggg tagacatgcg accatcagtg
     1681 tcgttacgcg gcgaattggc tggcctcggc agaagccaga tgtggggcca ctaccggtat
     1741 ggcggcttca ggccgttatg ccaagccttt tcgcaagctt ctgggtgaag ttccttgggg
     1801 tattccaaga tgatctcgat gtctgcctcg ctcatcgact cccacagatc tcagttcacg
     1861 atgtagcggc tgcaaccgtc cagaatgctg cacaggtaat agaaagttcc gctgatgttg
     1921 atgtacgaaa tgtcgatatg ccaatgttgg tgaactgcca gcggctgctc aagtgcttcc
     1981 ctctcgtgcc atcgcttccg gcctggtttc ctcattccat gctttgcgaa ccctctgctc
     2041 ccctcccgat aaccactccc ttgcggtcgt ggctcggaaa acgaatgtat tgacaacaac
     2101 ctcgtattaa catagactgt ctatgtgact ggcagacaga aacggcgcga tgagattccg
     2161 tacggcactc tcgacatgct gattctgaaa acactttcaa ctgccgggga gatgcacggc
     2221 tttcagattg ccgactacat tcaggaaaac accgagagcg tcctgaacgt ggaagaaggc
     2281 tcgctctatc cggcactcca gcgtttgctg gtgcacggct gggtgaccgc ggcgtggggc
     2341 cggacggaag gaaatcgccg cgcccgctac tatcgtctga cgcccgcggg gcgcaaacaa
     2401 ctggaacgcg agatggagca gtattaccgg ctgacgcatg ccatcggccg cattctacag
     2461 cccgcctgag gaggactcat gggcatcgcc gacatctggc gccggatcct ctttcttgtc
     2521 cggcgcaccc gcgcggacga agaactcgcc gaggaaatgc ggctgcacat ggagctgcgc
     2581 gccgccaagc ttcaacagca aggcgtcgaa gcggacgacg cggccctcac tgcacgccgt
     2641 agattcggca acacgaccgc gatccgcgaa accagcagag acacatgggg ctggcggtgg
     2701 ctcgaggatg cattgcagga tttgcgattc ggccttcgaa tgctcgccag gtcgccgggc
     2761 tttgcggcca tcgtggtgct gaccctcggg ctgggcattg gcgccagtac cgcgatcttc
     2821 aacgtggtga atggtgtgct cctcgcgggg cttccatttc aagaaccgga ccggcttgtc
     2881 gcgctgcatg agaggatgga aaagaggatc gatcgcgatt tccggttcag cccgcccgat
     2941 tacattgttc tgacccaaca gaaagattgg ttctcgtccg ttggttcctt ccggaatatc
     3001 caatatgaga tcacgggttc cggcactccc gagcgtgtca cgggagcaaa ggtcacggcg
     3061 agtctgtttg aaacgctcgg tgtggacgcg ctcatgggcc gcaccttcac ggatgcggaa
     3121 gatcacgaag gacaccaggt tgccttgttg agccatgcct actggaaacg gaagttcggt
     3181 caggacgcat cggtgatcgg ccgcggcatc cgcgtggacc gccggcttta cacgatcgtc
     3241 ggcgttcttc cgcccagctt cgtctttccg ccgcgaggaa tgccctccaa tgggacgccc
     3301 gccgacgttt tccttccgat gagcttcacg gccggacaga agcaggcgta cggcatgcag
     3361 tacagccaca gtgtcgtggc ccggctcaag ccagacatag gcattgaaca agtgagagcg
     3421 gccgcgccta tgctcgcgcg ccgtttcgag gaacgctatc ccgcattctt ccagaagatc
     3481 ccgggctttt cgctttcggt aacgattgcc ccgtttcgcg acaaaatcgt cggcaacgtg
     3541 cagacgctgt tactggtgat actcgcagcg gtgctgatgg tgttattgat cggctgtgcc
     3601 gatgtggcca gcctgatgct ggcgcgagcc accggccgcg cgcgagagat ggcgattcgc
     3661 gtggcgctgg gtgcgggccg tttccggctg attcgccagg tgctggtgga aagcggcgta
     3721 ctcgctttcc tgggcggcgc attcggcgtg ttccttgcga gtatatcgac aaacgccctg
     3781 atggctgcct cgccgatcga actcccgcgg ttggaagcgg tcgtcatgaa ccagcacgcg
     3841 ctcctgttcg cggccgcgat cagcatgctc accgcgctgt tgttcggact ggttccggcc
     3901 attgagagct cgcggggcga agccgcggat gcgctgcggg aaggcgggca tgggcggact
     3961 cccagtctgc gccgccgccg ctggctgaga tcgttcgttg ccgtgcaagt ggcattggcg
     4021 gtggtgctgg cgatcagtgc ggggctgctg atgaggagtt tcaccaagct gctggaagtc
     4081 aacccgggtt tccggccgca acaagtcttg gggttgtcgc tgaatctgcc cttcgaaagc
     4141 tacaggcagg ctgcgcaggt aaccggtttc tgggagcggc tgcgcgagca cctttccacg
     4201 attccaggcg ttgaagcggt tggcataggc gatctgccgc tggctgtccg tgagagccgt
     4261 gccatttggc cggaggacac gagcgcattc gaaagcaatc ctcctcagat tcgacagtca
     4321 tgggtgcacg gcgaatattt ccgcgcgctg ggcgtgccgc tcctcgaagg ccgctggttc
     4381 acggatcggg atgggaaaga ttcacagcgg gtgatcattc tgaatgaaac gatggcgcgc
     4441 atcttttatc ccggcaagaa cccgatcggc cagcgtttga aatggggagg ctccgccgaa
     4501 tccccgaatc catggatgac ggtcatcggc gtggtggggg acataaagca ggaatcactc
     4561 caggagagga cagcgcctat gacgtttaca cccttgatgc aggagccgga cgaagcgata
     4621 gcgcctccgc tcagcatgct gcgcgcgatg aacgttgccc tccgcacgag tggtgatccg
     4681 ccggcaatcg catcggccgt ccgcaagcgt gtcgccgggc ttgaccccgc gctgccggtt
     4741 gcggatatga aaacgatgga ggatgcggtg cgtcaggcgg cggcacccca acgtttcagt
     4801 acgtatctgc tgggcgcgtt cgcggcgatt gctctattgc tggcgacgct gggaattgcg
     4861 ggcgtcgtcg cttactcggt ggggcagcgc acgcgcgaga tcagtctccg cattgccttg
     4921 ggcgcttccc gaaggacgat tctctccctc acgttgcggg aaggcttgct gtatgcaggg
     4981 ctcggcctcg cggctggagc cggcctggcg ctcgggctga cgtggttaat gggctccctg
     5041 ctctatggtg tgcaggccac ggatgctgtg acgttcgccg gcgtgatggg tgttgtcggg
     5101 gtggtggccg cggcatccag tctgctgccg ggttatcgcg cgacgcgcct cgatccgatg
     5161 gttgcgttga gaaatgagta acaaacgcgg acggggccgg ctgcggtatc gtgataagtc
     5221 acgctatgct gcgactcgta ctcctctctc ttctgactct tcctctggcc gcgcaggacc
     5281 gcatcgccac ggttcaggtt cgcgtttcca cggaccggtc cgattggcgc tatgaacccg
     5341 gccagcctgt tcgcttccgc atcgtcgccg tcgaggacgg ccacgcgctg agcggcgtta
     5401 aagtgactta ccgggtcgga cccgaaatga tgccccctaa aatcgagcgg actgagacgc
     5461 tcaccgccga cggcctcatc attgatggcg gcacgatgaa ggagcccggc ttcctccgat
     5521 gtatcgccac cgtcgagcag aacgggaaga cgtaccgcgg actcgcaacc gccgcttttc
     5581 atccggaaag catccagccc acgcagaagg accctgccga cttcgatgca ttctgggccg
     5641 cgggcaaagc cgctctcgcc aagttgccca ttgacgcgag aatcaccccg ctgcccgaat
     5701 atggcaacgc ttcggccgat tgctacgagg tcaatctaca gaacatcggc atgggaaacg
     5761 cgccctcgcg tttctatggc attttgtgcg agccgaaagc gcccggcaag taccctgcgc
     5821 ttctcgccgt cccgggcgcc ggtgtccgtc cctatcgcgg tctggccgcc atggcggcgc
     5881 gcggcatcct cacgctacag gtagggattc acggcattcc ggtcacgctg gaccagtccg
     5941 tctacgactc gcttggcgcc ggtgccctga tgaactacaa caccttcgga ctcgaccatc
     6001 gcgaccgcta ctattaccgc cgcgtctatc taggctgtgt gcgcgccaat gactttctga
     6061 caagtcatcc gaagtgggac ggcgtcaacc ttgccgttac cggaggcagc cagggtggag
     6121 cgctctcgat cgttaccgcg gcgctggatc cgcgtgtacg gggcctggcc gcttactacc
     6181 cggcgctttc cgacgtcacc gggtatctcg agaatcgcgc cggcggttgg ccgcacatgt
     6241 tccgcgccgc cgatggcccg ctgtcccatc gctcgccgga caagatcgag acctcgcgct
     6301 attacgatgt cgtgaacttc gcccggcgcg tcaaagtgcc cggcctctac tcctggggct
     6361 tcaatgacca gacgtgccca cccacttcca tgtattcggc ctacaatgtg attcccgggc
     6421 agaagaaatt gcttctggcg ctcgaaaccg ggcacaacaa tattccggag caggttgccg
     6481 aggtgcaggc atggcttgaa gcgtttctga agacggcttc cgcgggaaac tgagaccggc
     6541 ggacacgtct attcaccgtg cactagtgca cggttgcgat cccggaagcg tcttcccttg
     6601 aagaggcact tctgacaaca tgaacagtat caacttgggg cagttgctca tctgcacgct
     6661 gctcgggcat gcacttgcgc agcctccgaa acctgcccat ataattccga acgatccgtt
     6721 attccggctc caatacagct tcttcaatcc caatcagaac agcgtccgct tcccggcagc
     6781 cagagccggc caacccgatg tcgaaatgcc ggcgacgccg gaggtggatt tggccgtaac
     6841 aaaagcctgg acgttgacga ccggcaaccg gagcgtaatt gtcgctcttc tggacgacgg
     6901 gttcttctac cagcacgaag atattcggga caacatatgg aagaaccccg gggagacagg
     6961 aacggatgca actggacgtc ctaaagaaac aaacggcgtt gatgacgatg ggaacggttt
     7021 cattgatgac gtggccggct gggatttcgc gttcgatgat cccgaccctg acgcgtatgt
     7081 cttcgatggt atggaccgga ctcgaattca acctttttgg cattcgatca gcgcactcgg
     7141 aatcatcggc gccaaaggca acaatggtac cggcgtggcg ggcattaact gggacctatc
     7201 gatgatgttg ctgaagattg gggcgcaggg aatccgccgg ggcgaaacgg atactaatcg
     7261 aaagactcgc gccgcgaggg ccattcggta tgcggttgac aacggggctc gtgtaatcaa
     7321 ttggagtggc ttcgtcgaag acaagtccaa cttagaggaa ctccagggag cgtttgaata
     7381 cgccgaacag cacggcgttc tgatagtcgt cgccgccggc aattcgggtc tcgacttgga
     7441 cgtgccgggt aatgcagggt accccggatc gttcacaaac tcgaatatca ttcgcgtagc
     7501 tgaactaact ttcaccggtg ggttggatcg gtattcaggg cgggaccgga taagcagttc
     7561 caacttcggt gctcgaacgg tggagattgc cgctattgga cggaattaca ctacagatgt
     7621 gcgcaacggc ctatcggcgt accgcctcag cggtggcaca tcgagtagcg cacccgtggt
     7681 aacaggcgtg gcggcattag ttctctcggt tcgccctgac ctccgtgcgg aacagatcaa
     7741 acagatcctc ctcagatctg cgagacgcct ccccgaaatt gaagggaagg tgatcgctaa
     7801 tgggataatc gatgcctacg ctgcggtcac gacagcgctt cggtggagcc gctaacccaa
     7861 cgcaacgtgc ggggacgcgc cgttacagac cgagattgac aggtgcgccg gctgtgtgac
     7921 agtctagctt cagcgcgctc tggggcgcct cctgtccgcg gcaagggagc ctgctcccac
     7981 ccaacctttg agtttgtgtt ccgtacggac ctttcatccg gccctgagat cagcggacct
     8041 gggcgacgac ggaaggaagg tatatgcagc gaaagctcac ctctgccacc actctcgaca
     8101 acctgagacg ggaagtcaag cgctggctta aagccctacg cgaagacaac ccggaagcgc
     8161 gcgaacggtt cgagcgcgcc tacccgaaac aaacgactac gcccgtgctg cgcgatgtgc
     8221 aatatgcgct cgcgcgggag tacggattcg agaactggaa agaactgaag ctcgccgtcc
     8281 agcaggcggc gagatcgcga acgcgcgagg tgtacgagcg ggccgcgcgt gacttcgccg
     8341 atgcttataa gggcgatgcc gcggcgctcg accggttgaa ccgccactat ggccggtcct
     8401 tcagcctcgc tgacctcaag gcggaaatct ggcggcgcga ctatgcgtac cgccagcgat
     8461 cgttccgtgc cccggagagc tacttccccg tagaagaagc acagatcatc gtcgcccagg
     8521 acgcggggtt cggcagttgg aaaaaactga tggacgccct cgcgactggc gtgccaccgc
     8581 agggtgctcc ttatgcgctc gacaccaaag acaaccgaat cgggccgcgc cgccgcatgc
     8641 cgggcactga ttgggacgag ttgatcgggg tcatgaaaga gcggcggatt ccggcgctgg
     8701 acgccaatgg cttcatgaca gacgaagcgc tggcccggat cgccgggctg aactttgtga
     8761 cgagcctgag cctgggaggg tcgcgcgaac taacggatga cggtctgcgg catcttgtgc
     8821 gcatgccgca gcttgagcat ctcgatctga acgagtatcc cggcggcaag ctcacagacc
     8881 gcggcctgga agttctccgc catttaccga acctacggaa gttcaagatg acgtggcaat
     8941 ccggggtcac agatcgcggc gtcgcgaatc tccgcttctg cgagcggttg gaggaagtca
     9001 acctgatggg ctcgccgaca ggggacggcg ggatcgaagc gcttcagggc aaggcgaaac
     9061 tacgccggtt cagcaccggg aggcttgtta cggatgccgg catttcgctg ctgcacaact
     9121 ttcctctgct cgaaaccgcc gatggcgaag gggcgcggct gctcgtcgac ggcccattca
     9181 ccaacaccgg gctggctgcg ctcgccggac tcgaaggcat attcgagctg gacctgttct
     9241 ggcacgttga tcgaatcacg acggacggct tcgcgcacct ggtccactta ccgaacctga
     9301 tggcgctcgg ctgcgacggc aagctgagcg acgacgttgc cctgaggcat atcgcggcga
     9361 tgcctcgcct gaagcggctg cgaatccagg agtcggttgc gaccgacgac ggattcgtcg
     9421 cgctggccag gtcccggaca atcgagggca tctgggggcg cgtctgccca aacttcggaa
     9481 gccggggatt cattgcgttc tcgaagatgc catcgctgcg gagcctgggg atcggctgca
     9541 agaacgtcga cgacgcggcg ctcgccttac tgccggagtt ccccaccctg cgagagttga
     9601 cgcccatcgg ctttcaggac gacggcttcc ggcacatcgg ccgctgccga aatctggagc
     9661 ggctgacgtg catgtactgc cgcgagaccg gcgacacggc gacggaacac atccgggagc
     9721 ttgcgatcaa gtactactac tccgggctga cgcagatcac cgaccggagc ctcgagattc
     9781 tcggcggcat ggaatcgctg gagcaggtcg acctgtacga atgcctgaag gtcaccgatg
     9841 ccggtctgcc gttccttgcc cgcttgccga gactccgcga ggtgcatctg gacagcctgc
     9901 ccggcgtcac actggaagga accaaagtct tccggccggg tgtacacgtt tattactcga
     9961 cgtgatgctt ggttactaag cgcccgggcc gggcagcgtg cgcgtgtggt tgaaggtata
    10021 ttcgcccagc agacgctcgc tgccggagtc gtcgaaggca accaggcggg cgaccattgt
    10081 ttccgacgag gcggcctttg cgtaagtaat ggactgctga aacgcaacgc ttcccgccgc
    10141 ggcatcgaag ggaatatcgg ccaggcggaa ccgttccatg ccgtgcttat cgcaaataca
    10201 gagatcgact cgcgttgcgg cgctcaaatc cgcggtcagc cgcccgatca aaaaatcgtc
    10261 ttcggccgta acggtgcagg caacgcttcc gcccgccgga ggggcgtact cgcgtatccg
    10321 cagaccctct tcggcggcac acttcagaaa agcgtcgctc acaaccataa gaagcgaacc
    10381 gtcacgcgcc aaatcacgga ttgcgttggc cagcaaaatc atttcgcgaa gacgtgtgcc
    10441 gcactcgtca caggcgaaga gatgctcctc gatagcctct tcttcggatt cgggaagtgc
    10501 ggcaagccaa taatcggcca gaatagcggc atcgaccggt ttcacgcagc tcacgcggct
    10561 gcctccatgc aatggcgcag ttgatggatg gcgcggtggc ggatcacacg cacgttggct
    10621 tcagaaaccc ccaggaaccg cgcgacctcc aagctggtct cttcgccgta gaaggacata
    10681 acaatcaccg cgcgttcgcg ttctctgagc ttttgaatgc agcgccgcag ttcctcccga
    10741 tccagccggg gtgttgacgt gggcgccggc gcgagcatat ccacaccgaa ctgctccagc
    10801 aggcgttcct ttctcagggt gctgcggcgg aggtcgagca cggtcatccg gcatgttccc
    10861 aatacaaatg aggtcagctt gccggagtcc cgcaggcggc ctgcccgtaa cgcttcgatc
    10921 gcaatgacga ggacctgctg aacaaggtcc tccgcggcgt gctcatctct caagtgccgc
    10981 aggccataga ggcggatacg gggagccaca cgccggaaca gttcttcctc ggccgccgga
    11041 tcctgcccgg tgccgatccg atgcaccagg tccgcctcgt tcaactcaat ttccactata
    11101 gatctatgca taaataatgc aacagtcaac gcaaaaaaaa ggggaagttt cgactgcttg
    11161 atgcaacctc ggagtttggc gggcgatcgg cggattcttt cgatggagcg gacaaggtcg
    11221 tccatcaact cttccacatt gtgagggcaa ccgttggcca gatctcgctt ggccaaggac
    11281 cagactccct cgtcggggtt gagttccggc gcgtaggagg ggaagtgctc gatgtgaagg
    11341 cggcgatggc gcatctgaag ttccgcgagc ggttgcccat gatgagtttg tgagttgtcc
    11401 aggatggcga tcacatgacc gcgcaagtgc cgcagcaggt cgcgaaggaa cacgcacacc
    11461 tcatcgtgcc caatattgtt gaagtagagc tgatagaaca gtcccaagcg ttggcgcttg
    11521 ggactgacgg agactcccga gatgacggag atcttgtcgc ggcggtaaca atgccgatgc
    11581 accggagtct ggccgcgcgg cgcccaggtc ttgactacat gcgggatcag caggaatccg
    11641 gattcgtcgg caaagacgat gtgggcgccc aaccgcgcag cgtttttttt actcgcggcc
    11701 attccttctg cttccagcgc tcgatctcct cctcgttccg ttcgaccgcg cgtttctccg
    11761 gcttttgatg actccagttc agagtgtgca tcaagcgccc gatatgcgcc cgatggtagc
    11821 tcactccaaa ttcgcaacgg atca
//
LOCUS       DASTMK010000133         7693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_102775, whole genome shotgun sequence.
ACCESSION   DASTMK010000133 DASTMK010000000
VERSION     DASTMK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102775"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2628)
                     /locus_tag="VFQ79_05430"
     CDS             complement(<1..2628)
                     /locus_tag="VFQ79_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0139126.1"
                     /translation="MNITEFFIRRAVTTTLLMAGISIFGVLAYLGLPVSDLPNVDFPT
                     IQVSASLPGANPETMANTVATPLEREFTSVQGISSINSTSGVGATRITLQFDLERNID
                     AAAQDVQAAISAAARELPDNMPQPPTYRKVNPAEQPILYLGLSSATLPLSEVDEYAQI
                     NMAQRISTVNGVAQVDVFGSQKYAVRVQADPDRLASLGIGIDEVRAALDQGNSNLPAG
                     TLDGASKAYTIETTGQLTSAAQFRPLIVTYRNGRPVRLHEIARVVDSVENNKTASWFN
                     GERSIVLAIQRQPGTNTVQIVDKIRELLPMLREQMPAGMNLGVLFDRSEPIRHSIWDV
                     QLTLIIAIVLVVGVIFVFLRNPSATVIPSLAVPLSLLGTFAVMKLLGYTLDNLSAMAL
                     TLCVGFVVDDAIVVLENIVRHREMGKGRLEAAIDGSREITFTIISMTISLVAVFIPVL
                     FMSGIVGRLLQEFAVVISVAILVSGVISLTLTPMLGSRFLYHAHSDKRSRLFDWSERQ
                     FDRTLHFYEWTLHAVLRARLATLLVSFLVVGVTGYLFVKIPKGFFPTEDTGFLFGSTE
                     ASEETSFEQMMGYQQQVAAAVSKNPYVESYSSSAGGGGAMGGGVNTGRIFIHLKDRKD
                     RPGAEEIVQQLRPLLAEIPGVNTFLQIPPAIRIGGTLTKSLYQFTLQDTDLKTLYEWS
                     PKAEASIAQLPGLQDVTSDLRIASPQITVDIDRDQSRTYGVTAADIENALYDAYGQRQ
                     VSTIYTASNQYYVILEVLPEYQRDPEALSQLYIRSSGGTGVARGALVPLASVATLRHE
                     VAPVQVSHYGQLPAVTVSFNLAPGVSIGQAVDRVDEAMRQINLPSTITTTFQGTAQAF
                     QESLRNMWVLLIVAILVIYL"
     gene            complement(2625..3908)
                     /locus_tag="VFQ79_05435"
     CDS             complement(2625..3908)
                     /locus_tag="VFQ79_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0139127.1"
                     /translation="MFRSLPAGRQVSCRIRVIAAGIGLLFVVGLNGCGTAAPEESSKQ
                     EGAAGPAPPVVVAEVRRQEVPLEIRAIGNIEAFSRVQVKSQVAGQIERVHFQEGDDVR
                     QGQILFQIDPRQFRQAVREAEAALANAKAALAQSEANYERDLAEAKNARSQANRYAEL
                     ASKGIIPRIQNEQYETSAIAAEKGAAASKVAIESARAAVEGAEAKLSDARLQLSYTTI
                     RAPISGRTGNLTQKAGNLVAVNADTPLVIINQITPAYATFSIPEQSLSELRRYADRGK
                     LLVEALPSQAQGPPAQGQVDFLDNEVDQSTGTILMKARFPNTDRRLWPGQFVNVVVRL
                     TRETANLVPTAAVRTSQTGNYVFVVKSDSTAEQRTVDAARTWNDFTVVGKGVEPGERV
                     IVEGQVRVRSGTKVQVVRTWEPTREQARNTGVARP"
     gene            3979..5916
                     /locus_tag="VFQ79_05440"
     CDS             3979..5916
                     /locus_tag="VFQ79_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0139128.1"
                     /translation="MKDRKGFLVLCAFYLCSAQGVYPQQPAARAPLPASLPGTLESER
                     GIFDRYRGSPVSPVNFSDSPRLETLIRAGTLYLSLRDAITLAIENNLDVELQRLTPII
                     AETDILRAQAGGSIRGITASVQEGPSGLGSTAQGAAGQTQGISTTPVETQSSQQSVST
                     VTATGASGPPVPSLDPALVGTIGWNRTNRPQTNTFITGTNELTSTSQLGDLRFQKGFL
                     WGGTASVGLDATRQNTNNLRANINPATTAGLAFTYVQPLLQGFGLAVNNRFIRIAKNN
                     RQVSDLVFEQQVMTTAYTVARLYWDLVSLNSELRVQEQSLALSERLLNDNILQEQAGT
                     LAPIDVVRARAEVAGARRDLTVAQTRVRQQETILLDYITRQTLDAQRLGTLRITPTDA
                     ISPPAQDPVQPFQDLVAEAERRRPELAAALLQVENSRISLTGTRNALLPSLDLVVNGR
                     SNALFGAISSLPPIPGTDGGLIDRTADPAFVGGAGTGLSQIFRARFPDYGVQLQLTIP
                     LANRAARADYTRDQLSARQQEIRLRQIEKQIGVDVSNALIAVEQSRAAHQAAEEARMF
                     QEQSLEAEREKYAVGVSTNFLVIQYQRDLAEAQSAEVAALADYAKARATLDRVTGGIL
                     ERYGISIVEAYRGRVSGPPRP"
     gene            complement(5926..7140)
                     /locus_tag="VFQ79_05445"
     CDS             complement(5926..7140)
                     /locus_tag="VFQ79_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139129.1"
                     /translation="MIAAVIKIAMRALGRNKARTFLTMLGIIIGVASVIAMVSLGQGA
                     QKQVQEQIQSMGTNMLIVSAGSQTSGGLRGGAGTTTTLIPEDVDALLRDAPAVAAARP
                     SVNASVTLVSGSQNWTTRAEGVGSQYPAVRNRTVSSGEFFSEADVRTAARVAIVGQTV
                     ANELFPGMDPVGQTLRVRNLPFRIVGVLEAQGQSQWGQDQDDTLLVPYTTAMKKLLSI
                     TYVPTIYVSAVSSSATSDAERQITEILRIRHNIRTGQPDDFRVRNLTEVAEAAEATTR
                     VMTMLLGSIAAVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGARSKHVQAQFLIESV
                     VLGFAGGLAGIVMGLGVSALLSTVFGWPQSISPLAAVGSALFSMAIGVFFGYYPAKKA
                     ASLDPIEALRFE"
     gene            complement(7137..>7693)
                     /locus_tag="VFQ79_05450"
     CDS             complement(7137..>7693)
                     /locus_tag="VFQ79_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139130.1"
                     /translation="VDVSGLDKDQRAGIRNGKIGFVFQGFNLLRRTSALENVELPLIY
                     SGVPAAERARRSRIALSIVGLAHREESHPNQLSGGQQQRVAIARALVNNPSLLLADEP
                     TGNLDTRTSLEIMGVFQALNRDAGITIILVTHEPDIADFASRVVTVRDGRIIEDRRVR
                     KVRDATQELAALPPAEEDFELEEK"
BASE COUNT         1529 a         2410 c         2280 g         1474 t
ORIGIN      
        1 caggtaaatc accaggatcg ccacaattag cagaacccac atattacgca gcgattcctg
       61 gaaagcctgc gccgtgccct gaaaggtcgt ggtgattgtg gagggaagat tgatctgccg
      121 catcgcttca tcgacgcgat ctaccgcctg cccgatggaa acgccgggcg ccaggttgaa
      181 cgataccgtg accgcgggaa gctggccata atgcgatacc tgcaccggcg ccacctcatg
      241 ccgcaacgtg gcaaccgatg ccagcggaac cagcgcgccc cgcgccacgc ccgtaccgcc
      301 gctgctgcgg atgtaaagct gtgagagggc ttccgggtcg cgctggtatt ccggaagcac
      361 ttcgagaatg acgtagtact ggttgctggc tgtataaatg gtcgagacct gccgctggcc
      421 atatgcatca tacagcgcat tctcgatatc ggctgccgtc actccgtagg tacgggactg
      481 gtcgcgatcg atgtcgacgg tgatctgcgg actggcgatg cgaagatcgg acgtgacatc
      541 ctgcaacccc ggcagttgag cgatgctcgc ttcggccttt ggcgaccact cgtaaagcgt
      601 ctttagatcc gtatcctgca acgtgaactg gtacaggctc ttggtgagcg tgccgccaat
      661 ccggatcgca ggcggaattt gcaggaacgt gttgacgccg ggaatctcgg caagcaaggg
      721 ccgcagttgc tgcacgatct cttcggcgcc cggcctgtct ttgcggtctt tgagatgaat
      781 gaatatgcgc ccggtgttca cgccgccacc catggcgccg ccgccgcccg cggaggagga
      841 gtaagactcc acgtaaggat tcttgctcac ggccgccgca acctgttgct ggtaccccat
      901 catctgctcg aacgaggttt cttcggatgc ctctgtgctg ccgaacagga agccggtgtc
      961 ttcggtcggg aaaaagcctt tgggaatttt cacgaacagg tagcccgtaa ctccgacaac
     1021 caggaaggag accaggagcg tcgccaggcg ggcacgcagc accgcgtgca gcgtccactc
     1081 gtagaagtgc agtgtacgat caaactgccg ctcggaccaa tcgaacagcc ggctgcgttt
     1141 atcgctgtgg gcatggtaca ggaaccggct cccgagcatc ggcgtgagcg tcagcgaaat
     1201 cacacccgag acaagaatcg ccaccgaaat cacgacggcg aattcctgaa ggaggcggcc
     1261 gacaataccg gacatgaaca acaccggaat gaacaccgcc accagcgata tggtcatcga
     1321 gatgatggtg aaggtaattt cccgcgaccc atcgatagcc gcttccagcc tgcccttccc
     1381 catttcccgg tgccgcacga tattttcgag cacgacgatg gcatcgtcga cgacaaagcc
     1441 gacgcatagt gtcagggcca ttgcggagag gttatccagc gtgtagccga gcagcttcat
     1501 tacggcgaaa gtccccaata gcgatagagg aacggcaagg ctcgggatga ccgtcgccga
     1561 gggattgcgc aggaatacga agatgacgcc caccaccagc acaatggcga tgatcaacgt
     1621 cagttgtaca tcccaaatag aatgccggat cggctcggag cggtcgaaca gaacgccgag
     1681 gttcataccc gccggcatct gctcgcgaag catcggcagc agctcgcgga tcttgtccac
     1741 gatctgcaca gtgttggtac cgggctgcct ttgaatggcg agcacgatcg atcgttcccc
     1801 gttgaaccag ctcgccgtct tgttattttc gacgctgtcc acgactcgcg cgatttcgtg
     1861 caggcgcacg ggacggccgt tgcggtaggt gacaatcagc ggacggaact gcgcggcgga
     1921 ggtaagctgg cccgttgttt cgatggtgta cgccttgctc gcgccatcca gcgtgccggc
     1981 ggggaggttc gagttgccct gatcgagagc ggcccgtact tcatcaattc cgataccaag
     2041 cgaagcgagc cggtcagggt cggcttgcac gcgaacggca tacttctgtg acccgaagac
     2101 atccacttgt gcgacaccgt tgacagtgga aatacgctgg gccatgttga tttgcgcgta
     2161 ctcgtccact tccgacaacg gcagcgtcgc cgagctcagc ccaaggtaaa gaatcggctg
     2221 ctcggcgggg ttcaccttgc gataggtggg aggctgcggc atattgtcgg gcagttcgcg
     2281 cgccgccgcc gaaatggcag cctgcacgtc ctgagccgcc gcgtcgatat tccgttccag
     2341 gtcgaattga agcgtgatcc gcgtcgctcc aacaccactg gtggagttga tcgaggagat
     2401 gccctggacc gacgtgaatt cccgttcgag cggagttgcc acggtatttg ccatggtctc
     2461 cgggttggcg ccgggcaggc tggcgctcac ctggatcgtc ggaaaatcca cgttgggaag
     2521 atcgctaaca ggtaagccga gataggcgag gacgccaaaa atgctgatgc ccgccatcaa
     2581 cagcgtcgtt gtgacagccc gccgtataaa gaactcggtg atattcatgg ccgcgccact
     2641 ccagtgttcc gcgcctgttc ccgggtgggt tcccatgtgc gcaccacttg caccttggtc
     2701 ccggatctca cccgcacctg cccttcgaca atgacccgct cgcccggttc cacgcctttg
     2761 ccaaccacgg tgaaatcgtt ccatgtgcga gcggcgtcaa ccgttcgctg ttcggctgtg
     2821 gaatcggact tcaccacgaa aacgtaattg ccggtttgtg acgtacgcac agccgcggtc
     2881 ggcaccaggt tggccgtctc ccgcgtcagc cgcacgacaa cattgacgaa ctggcccggc
     2941 cagagccttc gatcggtatt ggggaagcgg gctttcatca ggatggtccc ggtgctctgg
     3001 tccacttcgt tatccagaaa atcaacctgt ccctgggccg gcgggccttg cgcctgcgag
     3061 ggtagcgcct ccaccagaag ttttccccgg tctgcatacc ggcgcaactc gctcagggac
     3121 tgctccggaa tagagaaggt ggcataggca ggcgtgatct ggttaatgat gacaagcggc
     3181 gtgtcggcat tcaccgcaac gagattgccc gccttctggg tcaggtttcc tgtcctgccg
     3241 ctgatgggcg cccgtatggt ggtgtagctt aattgcagcc gcgcatcgga caacttcgct
     3301 tcggcgcctt ccaccgcggc gcgcgcactc tcaatggcta ctttcgacgc agccgcacct
     3361 ttttcggctg caatggcgct ggtttcgtat tgctcgttct ggattcgggg aatgatgcct
     3421 ttcgaggcaa gctctgcgta gcggttggct tgcgaacggg cgttcttcgc ctccgccagg
     3481 tcccgctcgt agttggcttc cgattgtgcg agagccgctt tggcattcgc cagggcagcc
     3541 tcggcttctc tgaccgcctg ccggaactgg cgcgggtcga tctgaaaaag aatctggccc
     3601 tgacggacgt cgtcgccttc ctggaaatgg acccgttcga tctgcccggc gacttgcgac
     3661 ttcacctgca cccgcgaaaa cgcctcgata ttacctatgg cccggatttc aagcggcacc
     3721 tcctgccggc gcacctcggc caccacaacg ggaggcgcgg gaccggcggc gccctcctgc
     3781 ttagaggact cctccggtgc ggctgtaccg cagccgttca atcccactac gaacaacaga
     3841 cctatgccgg ctgcgataac ccggatgcgg caactgactt gccgtccggc gggcaaactt
     3901 cgaaacacaa cgaacagacg ctacccctct ctacgatgtt acttaatccc ttgctaccct
     3961 gtcctacagt cacctgccat gaaagacaga aaaggctttc tcgttttgtg cgcgttctat
     4021 ctttgcagcg cacagggggt ttacccgcaa cagcccgccg cccgcgcgcc tcttcctgcc
     4081 agtttaccgg gtacgctcga gtccgaaagg ggcatcttcg atcgatatcg aggctctccg
     4141 gtttcgccgg tcaatttctc cgactcgccc cgtctggaaa cgctgatccg tgcgggaacc
     4201 ctttatctct cgctccggga cgctattacg ctcgccatcg agaacaacct ggatgtcgag
     4261 ctgcaacggc tgacgcccat catcgccgaa accgacattc tcagggctca agccggcggc
     4321 tcgattcgag gcattacggc aagtgtgcaa gagggcccct ccgggctggg ctccactgcg
     4381 cagggagccg ccggccagac ccagggaatc tcgacaacac ctgtagagac gcaatcctcg
     4441 cagcagtccg tgtctacggt aaccgcgaca ggcgcctcgg gaccgccggt ccccagcctc
     4501 gatcccgccc tggtgggaac catcggctgg aaccgtacca accgcccaca aaccaacact
     4561 ttcataaccg ggacgaacga actgacctcc accagccagt taggagacct gcgctttcag
     4621 aaaggcttcc tgtggggagg aactgcctcg gtgggcctcg atgccacgcg ccagaacacg
     4681 aacaacctgc gcgcgaatat taatcctgct accacggcgg ggctggcgtt tacctacgtt
     4741 cagcctctgt tgcaaggttt cggattggca gtgaataacc ggtttatccg aatcgcaaag
     4801 aataaccggc aggtctccga cctggttttt gagcagcagg tgatgaccac ggcctacact
     4861 gttgcacgcc tttactggga cctggtgagc ttgaacagcg aattgcgggt tcaggaacaa
     4921 tcgctggctc tttcggaacg tctgttgaac gataacatcc tccaggagca agccggaacg
     4981 ctggcgccca tcgatgttgt tcgagcccgg gcggaagtgg ccggagcacg gagggacttg
     5041 accgtggccc aaacccgcgt gcgtcagcag gagacgatcc tgctcgacta catcacgcgg
     5101 cagacactgg acgcgcagcg gctgggtacg ctgcgcatca cgcccaccga cgccatcagc
     5161 ccacctgccc aggatccggt tcaaccgttt caagacctcg tcgccgaagc ggaaagacgc
     5221 aggccggaac tcgccgccgc gcttctgcaa gtggaaaact cgcgcatttc gctcaccggg
     5281 acgcgcaatg cgctgcttcc ttccctcgat cttgtcgtga atggccgcag caatgccctc
     5341 ttcggcgcca tcagttctct gccgccaatt ccaggcaccg atggcggact catcgaccgc
     5401 acagccgatc cggcattcgt aggaggggcg ggtacgggcc tgtcccagat ttttcgagcg
     5461 cgatttccag attatggagt gcagttgcag ctcacgattc cgctggcgaa ccgggccgcg
     5521 cgggccgatt acacgcggga tcaattatcg gcgcgccagc aggaaatccg gcttcggcaa
     5581 atcgaaaaac agatcggcgt ggacgtgtcg aatgcgctca tcgcagtgga acagagccgg
     5641 gcggcgcacc aggcggccga ggaggcgcgc atgtttcagg aacagtcgct cgaggcggaa
     5701 agggaaaaat acgctgtggg agtttctacg aacttcctgg tgattcaata ccagcgggat
     5761 ctggctgaag cacagtcggc cgaggtggcg gcactggccg actatgcaaa agcgcgggcc
     5821 acgctggaca gggtaaccgg tggaatcctg gagcgctacg gcatctcgat cgtcgaggcc
     5881 tatcgtggcc gggtttcggg cccgccgcgc ccgtgatccc gataatcact cgaaccgcag
     5941 ggcttcaata gggtcgaggc tggcagcctt cttcgccggg tagtaaccga aaaagacccc
     6001 gatcgccatc gaaaacagcg ccgagcccac ggcggcgagc ggcgaaatgg actgtggcca
     6061 gccgaatacc gtcgatagca gcgcggacac accgaggccc atcacaatgc cggctaaacc
     6121 tccggcgaac ccaagcacca ccgactcgat caggaactga gcctggacat gcttcgagcg
     6181 cgcacccacg gccattcgga ttccgatttc ccgcgtgcgc tcggtaaccg acaccagcat
     6241 gatgttcatg atcccgatgc cgcccaccaa tagcgaaaca gcggcaatgc ttcccagaag
     6301 catcgtcatg acgcgcgtcg tcgcctccgc ggcctccgcc acttccgtaa gattgcggac
     6361 ccggaagtcg tccggctgac cggtgcgaat gttatggcgg atgcgaagaa tttcggtgat
     6421 ctgccgctcc gcgtcgctgg tggcggagga actcacagcc gaaacataaa tcgtcggcac
     6481 gtaagtgatg gacaggagct ttttcatcgc cgtcgtatag ggcaccagga gcgtatcgtc
     6541 ctgatcctgc ccccattggc tttgaccttg tgcttcgaga acccccacga tgcgaaacgg
     6601 gagattgcgg acgcgcagcg tctgtccgac ggggtccatg ccggggaaga gctcgttggc
     6661 cacggtctgc ccgacaatcg cgacgcgggc cgcggtccgc acatccgctt cactgaagaa
     6721 ttcaccgctc gacaccgtgc ggttccgcac ggcgggatat tgcgagccga ctccttcggc
     6781 gcgcgtggtc caattctggc tgccggagac aagcgtcacc gaagcgttca cactgggacg
     6841 tgccgccgcg acggcaggcg cgtcccgcaa cagcgcgtcg acgtcctcgg ggataagcgt
     6901 tgtcgtggtg cccgcgccgc ctcgcaatcc tccgctcgtc tggctgcccg ccgagacgat
     6961 cagcatgttt gttcccatgc tctggatctg ctcctgcacc tgtttctgag cgccctggcc
     7021 gagactcacc atcgcgatga ccgaagccac gccgatgatg atgccgagca tggtcaggaa
     7081 cgtccgcgcc ttgttacggc ccagcgctcg catggcgatc ttgatgacag cggcgatcat
     7141 ttctcctcca gttcaaaatc ctcttcggcg ggcggaagcg ccgccagctc ttgtgttgca
     7201 tcgcgcacct tgcgcactcg ccggtcctcg atgatgcgcc cgtcccgaac ggttaccacg
     7261 cggctggcga aatcggcgat atcgggctcg tgcgtcacca ggatgatagt gataccggca
     7321 tcgcggttca acgcttgaaa aacacccata atctcaaggg aggtccgcgt atcgaggttc
     7381 cccgtgggct cgtcggcaag caacagcgac ggattgttga ccagcgcccg ggcgatggcc
     7441 acgcgctgct gctggcctcc ggaaagctgg ttcggatggc tctcttcgcg atgcgcgagg
     7501 ccaacaatgc tcaacgcaat acggctgcgg cgcgcgcgct cggcggccgg cacgccggag
     7561 tagatcaagg gtaactcaac attttccaga gcgctggtcc tccgcagcag gttgaatccc
     7621 tggaatacga acccgatctt tccattgcgt atgccggcac gctggtcttt atcgaggccc
     7681 gatacgtcca cgc
//
LOCUS       DASTMK010000134         7486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_230848, whole genome shotgun sequence.
ACCESSION   DASTMK010000134 DASTMK010000000
VERSION     DASTMK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230848"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VFQ79_05455"
     CDS             <1..1164
                     /locus_tag="VFQ79_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139131.1"
                     /translation="KLPNARVAAVCDIDDAQNERAIAFTQKQQQGLPPRSYRDLRKLL
                     EDKDIDAISIATCNHWHALATIWACQAGKDVFVEKPASHNIFEGRKMVEAARKYNRIV
                     QTGMQSRSIEHKIQAIQLLRDGAIGKVYMAKGLCYKQRKSIGKQADGPVPAGVDYDLW
                     LGPAPMRPFNPNRFHYNWHWFWDTGNGDIGNQGVHEMDVARWGLGKDTLPGNVHSGGG
                     KFVYDDDQETPNTQLATFDYGDCELVFEVRGLIAGTEGNIAPEGAHYIGNLFFGSEGY
                     MSVDSGGFRIYLGEERKLAREVMHAEPRIWDTYPLVANFIKAVETRKHQDLTCDIVEG
                     HLSAALCHMANASYRTKRKLTFDPATEQFQDDPEANACITRKYRPPYVVPENV"
     gene            1161..2084
                     /locus_tag="VFQ79_05460"
     CDS             1161..2084
                     /locus_tag="VFQ79_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0139132.1"
                     /translation="MTPRAAIMPRYHVNANSLNVRSTPQVRNDNKIAVLPFGHLVEKV
                     ADAGGWWKIQTRLQEAAIEGFVSSEFLSAEDGFTAPQAATSIRPVHLEENNPKITRVS
                     SSGRAFPLGEPAMPLRTGTSPEEKRAQLAEIVRFLDVEKNARYTPEPKATYCNIYAYD
                     YCYLARVYLPRVWWTEGAIAQLARGEPVFPSYGHTVRELSANALYDWLSDFGNLFGWK
                     RATSLEELQGAANQGAVCVICAQRTDRNQSGHISAVVPEAPPNAARRKGNDVRLPLQS
                     QAGRKNFCYSCGITSWWQGTQFGRFGFWIHP"
     gene            2097..3653
                     /locus_tag="VFQ79_05465"
     CDS             2097..3653
                     /locus_tag="VFQ79_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0139133.1"
                     /translation="MNAAEALVEGLQQHGVEWMATLCGHGLDPLLHAAKRAGLRLVDV
                     RNEQTAGYMADACGRLTRRPGVCAVSSGVAHINALTGVANAWFDQAPMLLISGAGALS
                     TAGRGHFQEMDQVALAAPITRYARVIESADRTLEILEEALAAAVRPEPGPAHLTFPLD
                     VQVTEAGAAAGRRKSVAEVAEGAAGDGPEAVAETLSKCRRPLIIAGSGVYYAGSGDEL
                     MRFSEQFSIPVVVPIWDRGSIERPIETFLGVIGAASGGPRLLGDSDCIVMAGALPDYR
                     VGYLETGAVMDGAAIVRMTQGWDELGAALGRRRATPFKAWLGEARKRREAFCRAVKER
                     AIRPASSGTHAVHIVQALREVMESTTVLLIDGGSIGQWAHQLLLDRYPGHWLTCGRSG
                     VVGWGIGGAMAARLVYPDRPVVLLSGDGAFTFNVAELERAARQELPFVAIVADDQGWG
                     ITRTGHIRQFGEPISSSLGAIDFAELAESLGARGVLIRKSEELVPALRDAISSRSVTV
                     LHVPIVGGNP"
     gene            complement(3666..4991)
                     /locus_tag="VFQ79_05470"
     CDS             complement(3666..4991)
                     /locus_tag="VFQ79_05470"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU0139134.1"
                     /translation="MRGFGPQFMSNVIVLGAQWGDEGKGKVVDLFSERFDIVARYQGG
                     HNAGHTVFIGEKKFILKLIPSGVLRGKMAVIGNGVVIDPGALIEEMETLEKAGIQVGK
                     QLAISNRAHVIFPFHRMVEKVSEARENRVAIGTTSRGIGPCYEDKIGRRGIRMADFID
                     RDQFHALYDLLAEDKQTLAKAFQVEEQIDFARIRDQYEEYAERIRPMVCDAAGLLNTA
                     MRDGKRVLFEGAQGTMLDVDHGTYPFVTSSSATAGGACTGAGVPPTRIDGVIGVSKAY
                     ITRVGGGPFPTEVQGEAGEKIRTAGKEFGAVTGRPRRCGWFDVPLLRYTSMINGFDSV
                     VITKLDVLDGFAEIPVCVAYKVNGREISDMPASIRDVESIEPVYEKLPGWCGPTFGMT
                     DYNGLPDRAKDYLKFLAGRAGVDIGCISTGPERDETIVIPNSKLASLIG"
     gene            5024..5485
                     /locus_tag="VFQ79_05475"
     CDS             5024..5485
                     /locus_tag="VFQ79_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139135.1"
                     /translation="MRLSDAFLSLGEEPFGALLRSISIGKLKTYQMYEGLKVRLHLNK
                     LNAETIRKAAPRLWTRLNERDEEFAQDLAQAILVSHIGMIRAVLDFLGIPNEEGFFAK
                     DVDATPYLTEGWQERVYEKFKDDFPRPLLLFYVNHLAWELARESHIFAPAA"
     gene            5785..6411
                     /locus_tag="VFQ79_05480"
     CDS             5785..6411
                     /locus_tag="VFQ79_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139136.1"
                     /translation="MGNDHSDDPNTRTLAIIAGYEQPLTSEIMAEMRKETEKVVLVPD
                     LNIVWRQLDANRPQEVFDHVLVARFRGSCGVADLPLPAGFDNVLGFTHLSDGVILPFV
                     EVDCERVRGLLRREFQRRSQTVRELLLGRALARVLAHELYHVLAGTAKHSARGIAKAH
                     MSPDDLVCDELAFEDGDLAKIRMHIFPNLELAAQPAVPLENRSAKQAE"
     gene            complement(6355..7167)
                     /locus_tag="VFQ79_05485"
     CDS             complement(6355..7167)
                     /locus_tag="VFQ79_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU0139137.1"
                     /translation="MESILAFLAGLLIGSFLNVCIYRLPRDLSVVRPRSFCPSCKQTV
                     AWYDNLPILSYLLLFGKCRHCGSRIPVRYPVVELLTAFAFGWIVHGHGATGPAIKLCV
                     FAALLIALVFADLEQRILPDELTLGGAAAGVLFASFVPLNGFLVFFLPAGLNAALASM
                     AESIFAALLGAAALWLVGELYYRIRGREGLGFGDVKLVAMIGAFTGLEGMLLTLLAGS
                     VAGALLGILYILVTRKDPSSYELPFGSFLGAAALLLSLFGGPVFEWYGGLGG"
     gene            complement(7168..>7486)
                     /locus_tag="VFQ79_05490"
     CDS             complement(7168..>7486)
                     /locus_tag="VFQ79_05490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0139138.1"
                     /translation="YITRQEGWGSGVLLAMAHGVPVIASGVGGLLEVVQPERSGLLVD
                     NQPAAIAGAMRRLLSDASLRALLAEGGYRRVREHFSEEQMINGTLEVYDDVLSGKYGE
                     PAT"
BASE COUNT         1559 a         2248 c         2233 g         1446 t
ORIGIN      
        1 aagctgccga atgctagggt ggccgcggtg tgcgatatcg acgacgcgca aaacgaacgc
       61 gccatcgcct tcacccaaaa gcagcagcaa ggcttgcctc cgcgttctta ccgggacctg
      121 cgcaagctgc tggaagacaa agatatcgat gccatctcca ttgccacctg caatcactgg
      181 catgcactgg cgacgatctg ggcgtgtcag gcgggcaaag atgtgttcgt ggagaaacct
      241 gccagccaca acattttcga aggccggaag atggtggagg cagcgcgcaa atacaaccgc
      301 atcgtgcaaa ccggcatgca gagccgcagc atcgagcaca agatacaggc gattcagctt
      361 ttgcgcgacg gcgcgattgg caaggtctat atggctaagg ggctgtgcta caaacagcgg
      421 aaatcgatcg ggaagcaggc ggatggcccc gtcccggcgg gcgtcgacta cgatctgtgg
      481 ctcggtcccg ctcccatgcg gccgtttaat ccaaaccgct tccattacaa ctggcactgg
      541 ttctgggata ccggcaacgg cgacatcggg aaccagggcg tacatgagat ggatgtggcc
      601 cgatggggtc tgggcaagga cacacttccc gggaacgtcc actccggcgg aggcaagttc
      661 gtctacgacg atgaccagga gactccaaac acgcagttgg cgacattcga ttacggcgac
      721 tgcgagctgg tctttgaggt acgtggcctc atcgccggga cggaaggcaa cattgcaccg
      781 gaaggcgcgc actatatcgg caacctgttt ttcggaagcg aaggatacat gtccgtcgat
      841 tcgggcgggt ttcgcattta cctgggcgag gaacgcaaac tggcgcggga agtgatgcat
      901 gccgagcctc gcatttggga tacctatcct ctcgtcgcca atttcataaa agcggtggaa
      961 acacggaaac accaggacct gacgtgcgac attgtcgagg ggcatctctc ggcggcgctt
     1021 tgccacatgg caaatgcaag ctaccgcacg aaacggaaac tcacgtttga ccccgccacg
     1081 gaacagttcc aggacgatcc cgaggcgaat gcctgcatca cgcgcaaata tcgcccgcca
     1141 tacgtcgtac cggaaaatgt gtgacgccca gagccgccat catgcctcga taccatgtaa
     1201 acgcgaacag cctcaatgtc cgttcgactc cgcaggtacg gaacgataat aagatcgccg
     1261 tcctgccgtt cgggcacctt gtagaaaaag tcgccgacgc cgggggctgg tggaaaatcc
     1321 agacccggct gcaggaagcc gccatcgaag gatttgtgtc gagtgagttc ctgtccgccg
     1381 aagatggctt tacggctccc caagcagcta catcgattcg cccggtgcac ctggaggaga
     1441 acaacccgaa aatcacccgg gtctcctcgt cgggccgtgc gtttcctctc ggcgagccgg
     1501 cgatgccgct ccggacggga acatcacccg aagagaaacg cgctcaactt gcagagatcg
     1561 tccgtttcct cgacgtcgag aagaacgccc gctacacgcc ggagcccaaa gcgacctatt
     1621 gcaacattta cgcctatgat tactgttacc tggcgcgcgt gtacctgccg agggtatggt
     1681 ggacggaagg cgcgatcgcg cagcttgccc gcggtgaacc agtctttccc agctacggac
     1741 atacggtccg cgaactgagc gcgaacgcgc tttatgactg gctgagcgat tttggcaacc
     1801 tcttcgggtg gaaacgcgct acgtcgctcg aagaacttca gggagcggcc aatcagggcg
     1861 cggtgtgcgt gatctgcgca cagcggaccg acaggaacca gtccggtcac atctccgccg
     1921 tcgttccgga agcccctcct aacgctgccc ggcgaaaagg aaacgatgtt cgtcttccgc
     1981 tccagagcca ggcgggacgg aagaacttct gttattcctg cggcattacg tcctggtggc
     2041 agggcacgca gttcgggcgg ttcggttttt ggattcatcc ttgacagggg acggttatga
     2101 acgcagcaga agcacttgtc gaaggtctgc aacagcacgg cgtcgagtgg atggctaccc
     2161 tttgcggcca cggcctcgat cctctattgc atgcggctaa acgcgccggc ttgcggctgg
     2221 tggatgttcg caatgagcag acggccggtt acatggcgga cgcctgcgga aggctcacgc
     2281 ggcggccggg tgtatgtgcc gtctcgagcg gggtcgccca catcaatgcg ctgaccggcg
     2341 tggcaaacgc ctggttcgac caggcgccaa tgctgctcat cagcggcgcg ggggcgctct
     2401 ccaccgccgg ccgcggccac ttccaggaga tggaccaggt tgctttggcg gcgcccatca
     2461 cacggtatgc gcgtgtcatt gaatccgcag accgcacgct ggagattttg gaggaagcgc
     2521 ttgccgccgc tgtgcggccg gaacccggtc ccgcgcatct gacgtttccg ctggatgttc
     2581 aagtgaccga agcgggcgct gcggccggaa ggcgcaaatc tgttgcggaa gtagctgaag
     2641 gcgcggccgg agacggtccc gaagcggtgg ctgagactct ctcaaaatgc cgccggcctt
     2701 tgattatcgc cggttccggc gtctattacg ctggctcggg cgacgagctg atgcgatttt
     2761 cggaacagtt ttccattccg gtggtggttc ccatttggga tcgcggctcg atcgaaagac
     2821 cgattgaaac gtttctgggc gtcatcggag cagccagcgg cggcccgcga ttgctcggcg
     2881 attccgactg tatcgtgatg gcgggcgccc tgccggatta ccgcgttggg tatctggaaa
     2941 ccggcgccgt catggacggt gcggccatcg tgcgaatgac ccagggctgg gacgaactcg
     3001 gcgcggcctt gggacggcgg cgcgccacgc cgttcaaggc gtggctgggg gaggcgcgga
     3061 agcggcggga agcgttttgc cgcgcggtga aggagcgggc gatccgcccg gcgtcctcgg
     3121 gcactcacgc agtgcacatc gtccaggcgc tgcgtgaggt aatggaatcg acgacggtgc
     3181 tgctgatcga cggcggcagc atcggccagt gggcacatca gcttctactg gaccgttatc
     3241 cgggccactg gctcacctgc ggccggagcg gtgtggtggg atggggcatt ggcggcgcca
     3301 tggcggccag gctggtttat cccgatcgtc ccgtcgtact gctctccggc gacggcgcat
     3361 tcaccttcaa tgtcgcagaa ctcgagcgcg ccgcccgtca ggaattaccg tttgtcgcca
     3421 tcgtcgcaga cgatcagggt tggggcatca cgcgaacggg ccacatccgc cagttcggcg
     3481 aacccatcag cagttcactt ggggcgatcg acttcgcgga actggccgaa tctctgggcg
     3541 cccggggagt gctcatccgg aaatccgaag aactggtccc ggcgcttcgc gacgcgatct
     3601 ccagccgcag cgtcaccgtg ctgcatgtgc ctatcgtggg cggcaatccg taagcaagac
     3661 aggtgtcagc cgatcaggga tgcgagcttc gaattcggaa tgacgatggt ttcgtctcgt
     3721 tcaggacccg tcgaaatgca gccgatgtcc acgccggcgc gcccggcgag gaacttcaga
     3781 taatccttgg cgcgatccgg caggccgtta taatccgtca tgccgaaggt cggaccgcac
     3841 cagcccggca gcttctcata cacgggctcg atcgattcca catcgcgtat ggatgcgggc
     3901 atatcggaaa tctcgcggcc gttcactttg taggccacgc agacgggaat ctcggcaaag
     3961 ccgtcgagca catccagctt cgtgatgacc acggaatcga acccgttaat catgctggta
     4021 tagcggagca gcggcacgtc aaaccagccg cagcggcgcg ggcgccccgt gacagcgccg
     4081 aattccttcc ccgcggtacg gatcttctca ccggcttcgc cttgcacctc ggtgggaaaa
     4141 ggtccgcctc ccactcgtgt gatgtacgcc tttgaaacgc ctatcacgcc atcgattcgc
     4201 gtcggcggca ctcctgcgcc cgtacaggcg ccaccggccg tcgcgctgga tgaggtgaca
     4261 aaggggtacg tgccatgatc gacatcgagc atcgtgccct gcgcgccttc aaacagcacg
     4321 cgcttgccat cgcgcatcgc ggtgttcagc aacccggcgg cgtcgcatac catcggccgg
     4381 atccgctccg catactcttc gtactggtcg cgaatgcgcg cgaagtcgat ctgttcttcg
     4441 acctgaaagg ccttcgcaag agtctgcttg tcttcggcca gcaggtcgta gagcgcatga
     4501 aactggtcgc gatcgataaa gtcggccatg cgtatgcccc gccgtccgat cttgtcttca
     4561 tagcaggggc cgatgccgcg cgatgtggta ccgatcgcga cgcgattctc gcgcgcttcg
     4621 gagacttttt ccaccatccg atggaagggg aaaatcacgt gcgcgcggtt gctgatcgcg
     4681 agctgcttcc ccacctggat gccggccttt tcaagcgtct ccatctcctc tatgagagca
     4741 ccggggtcga tgacaacgcc gttgccgatg acggccatct tgccgcgcag gacgccgctc
     4801 ggaatcagct tcagaatgaa tttcttctcg ccgataaaca cagtgtgccc ggcgttatgc
     4861 ccgccctggt agcgggcgac gatatcgaag cgctccgaga aaagatcgac gactttacct
     4921 ttaccttcgt cgccccattg cgcacccaaa acgatcacat tgctcatgaa ctgtggacca
     4981 aaacccctca ttgtatcagc ccgctatgct acgctggcgg ataatgcggc tctccgacgc
     5041 ctttctaagt cttggtgaag aaccgtttgg cgcgcttttg cgaagtattt ccatcggcaa
     5101 actgaagacg taccagatgt atgaagggct caaggtgcgg cttcatctga acaagctcaa
     5161 tgcggaaacc atccggaagg cggcgccccg cttgtggacg cgcctgaacg agcgcgacga
     5221 agagtttgcc caggatctgg ctcaggcgat tctggtgtcc catatcggga tgattcgtgc
     5281 cgtcctggat tttctgggta tccccaacga ggaaggattt ttcgccaaag acgtggatgc
     5341 gacgccgtac cttaccgaag gctggcagga acgtgtatac gaaaagttca aggatgattt
     5401 cccccgccct cttctgttgt tctacgtgaa tcaccttgcc tgggagctcg cccgggaatc
     5461 gcatatcttc gctccggctg cctgatagcc ccgattgaag aaaaccgcca ctcgcaaaat
     5521 tccgttcaca cacttcggcg tgcggctttt ctctttaact ctcaagcact tacgtaaaac
     5581 cctcggccgc accgggcaag tccggctgct acatttcggc caggggtaag gcattccgtg
     5641 tccctcaggg cctttctgta gaagtcagta ttatttccta caaaggataa agggctctga
     5701 tacactgaga tttctgccga tagaaatgct gtgagacagg tcctaataac tttacttctg
     5761 ataacatcgg cttccacaac tcttttggga aacgatcatt cggatgatcc caatacccgg
     5821 acactggcaa tcatcgcggg ttatgagcag ccgctcacat ctgaaatcat ggcggagatg
     5881 cgcaaggaaa ccgaaaaagt ggttcttgtg cccgacctca acatcgtctg gaggcaactc
     5941 gacgccaacc gcccacagga agtttttgat cacgtgttag ttgcgcgctt tcgcggatct
     6001 tgcggagttg ccgatcttcc actcccggcg ggcttcgata acgtcctggg gtttacacat
     6061 ctttccgacg gagtcatcct gcctttcgtg gaggtggatt gcgagcgggt ccgcggtctc
     6121 ttacgccggg aattccagag aagatcgcaa acggttcgcg agttgctgct cgggcgggcg
     6181 ctggcgcgtg tcctggccca cgagttgtac cacgtccttg cggggaccgc caaacatagt
     6241 gcgcgcggta tagccaaagc gcatatgtcg ccggatgacc tggtttgcga cgagttggcg
     6301 ttcgaggatg gcgacctcgc caagattcga atgcacattt tcccgaatct ggaactagcc
     6361 gcccagcccg ccgtaccact cgaaaaccgg tccgccaaac aggctgagta gtagcgctgc
     6421 cgcgcccaga aaacttccga agggcagttc gtaggaggag gggtccttgc gcgttacaag
     6481 gatgtacaga atccccagga gagcgccggc cacggagccc gccaaaagcg tcagcagcat
     6541 tccttccaga cctgtaaagg cgccgatcat agccaccagt tttacgtcgc caaacccaag
     6601 gccctcgcgg ccgcggatgc ggtaatataa ctcgccaacc agccaaagcg cggcggcgcc
     6661 cagtaaagcg gcaaaaatcg attctgccat cgacgcgagt gcggcattca agccggcggg
     6721 cagaaaaaag actagaaatc cgttcagagg gacaaacgac gcgaacaata caccggctgc
     6781 cgctccgccc agcgtcaact cgtcaggaag aatccgctgc tcgaggtcgg caaaaaccag
     6841 cgcgatgagc aatgctgcga acacgcacag tttgatcgcc ggcccggtcg cgccatgtcc
     6901 gtgaacgatc catccaaatg caaacgcggt gagcagttcc acgaccggat aacggaccgg
     6961 aatccggctc ccgcaatgcc ggcattttcc gaagagcaga aggtaactga ggatgggcag
     7021 gttgtcatac caggcgacag tctgtttgca ggaaggacag aacgagcgcg gccgcaccac
     7081 ggagagatcg cgtggcaggc ggtaaatgca tacgttcaga aagctcccga tcagcaggcc
     7141 tgccaggaat gcgaggatcg attccattta tgttgcgggc tcaccgtact tgccgctaag
     7201 cacatcatca tatacttcaa gagttccgtt gatcatctgc tcttcgctga aatgctcgcg
     7261 gacgcggcgg tagccccctt cagccagcaa cgcccgcaaa gaagcatccg acagaaggcg
     7321 ccgcatcgcg ccggcgattg ccgccggttg attgtccacc agcagcccgg aacgctccgg
     7381 ttgaacgact tcgagcaagc cgcccacgcc actcgcgatc actggtactc cgtgcgccat
     7441 tgcgagtagc actcctgagc cccaaccctc ctgccgtgtg atgtag
//
LOCUS       DASTMK010000135        37260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_358974, whole genome shotgun sequence.
ACCESSION   DASTMK010000135 DASTMK010000000
VERSION     DASTMK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 37260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37260
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358974"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1910
                     /locus_tag="VFQ79_05495"
     CDS             <1..1910
                     /locus_tag="VFQ79_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0139139.1"
                     /translation="ENMSAPSRRSILGGAIAGSALSAVAARPSVGFPVERVFAGLTLI
                     RGPVNSAIFERNGKLLVIDSGSLGSRELGGVPQWLLFTHHHRDQAMGADKLVVAGARA
                     IAPDAEAHLFERAPEVWEKADSVIYHRYKFRPHLFTLYNAVPLAKRVRDRDVHEWEGL
                     RFEVIDTPGQTDGSVTYLVETEGCRIAFTGDLIYGPGQIQNMYSLQKTWPGMPGGYWG
                     FGGAVPDLKASLRRVLDRSPDMLIPSHGKPMRDPRKAVEELNHNLDMVMKNYLTTTAW
                     RTFFKQVLPPEAEPRMLQPMSKPDYPSWFRDIAVTTKAIVAGDKSVFLFDCGFSAPVS
                     KLVELQKNGEIGRIEKLWITHYHDDHVDCVNRIKERFGAELYVQEEMADIIEHPEAYN
                     MPCLSREPARVDRVLRDGEVFEWKGFRMTPYYFPGQTLYHAALLVEKGGVRLLLTGDS
                     FGNWGIDDYCSENRCFVGSGRGFEKCLELLLRLKPDMLVNAHSGAIRFSEDYVLKTLE
                     VFRQREMLYRKLLPWDNPNFGLDPFWLRAYPYKQSALPGSRVELEVRVLNHSDQTRMV
                     QASLVLPDGWRPVVVSGTSRLAAGQEGRIRLSAIAPETRNPRRYVLGLSAVFNGTPVG
                     EVAESIVDLLN"
     gene            complement(2321..2914)
                     /gene="rdgB"
                     /locus_tag="VFQ79_05500"
     CDS             complement(2321..2914)
                     /gene="rdgB"
                     /locus_tag="VFQ79_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU0139140.1"
                     /translation="MKLYCATTNPGKLREFRIAAEQRGIGIEPVPGLERIAPFEEAAL
                     SFEQNAAEKAAYYSRFAPGPVFTDDSGLEVEALGGDPGVLSARFAGENATDEENNRLL
                     LERLQGVTGRHARFVCVIAVARAGELLRLFRGAVEGRILEAPRGSNGFGYDPLFLYPP
                     FGRTFGEVTDEQKMRVSHRSQALRAMLDWYTEAPLAG"
     gene            complement(2911..3630)
                     /gene="rph"
                     /locus_tag="VFQ79_05505"
     CDS             complement(2911..3630)
                     /gene="rph"
                     /locus_tag="VFQ79_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU0139141.1"
                     /translation="MRIDNRQPAELRPVTITTGYLLTAEGSAIIEIGNTRVLCAASVE
                     DSVPSFLRGTGKGWVTAEYSMLPRATATRTAREVNRGRPSGRTLEIQRLIGRSLRSIV
                     DPVALGERTVILDCDVLQADGGTRTAAITGAFVSLSLAIRQLLQFGVLKKSPIRDSVA
                     ATSVGILQGELLLDLCYEEDSRAEVDMNVVMTGAGRFVELQATAEQTPFDDGRLLELI
                     DLARGGIQQLTAAQKKVLESA"
     gene            complement(3640..3780)
                     /locus_tag="VFQ79_05510"
     CDS             complement(3640..3780)
                     /locus_tag="VFQ79_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139142.1"
                     /translation="MARFKPAKGQKKKSASSARGAIPCLLVLLGAMLLMYLLFVSVLS
                     PR"
     gene            complement(3799..5685)
                     /locus_tag="VFQ79_05515"
     CDS             complement(3799..5685)
                     /locus_tag="VFQ79_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139143.1"
                     /translation="MLLALYHDNPSSQSAFQIFQEKNVFERLKALDSRIQNKGLGDLD
                     PARNTLIRIGMETDGSARIIRNSYGVFNLAWRAQEHPEWPDQVLRELEEIRGRIADTH
                     GVPLRYVIWAGMGGSAEDKSMYQTVGLLRRGPRLYVLDSTDPAKMKAVLADIVHRSRL
                     PLEKVLPQVLVVGMAMGMTSYEPVVNLERLYRLYKRHKIDSRPNFVYLTLTGSLLDQF
                     AGPRGYRRVELQLDGGNTTAGRHSAPLTRGSLYPLGLAKNDLRLWIDATLLTQEEIDT
                     AWRLASFLHEQGKAGRDKVALLLPREWSGAALWTKQNFEESLGKSEDLGLKIVIGEKP
                     KLANYVSPKLGRQDRVFLALSVKGLTPPDSQKTTMLRRAGYPLATLVAPRGTLLSHYM
                     QFIHYVVFGVAYLRGMNFVTQPSVELYKSITGRLHGDAVKAGAVESTREWQAMQNSSC
                     QLKWRGGITFAWDRLALPVDPAGRDAPAVYAGILRKLFCPRETEYGELTFFGDTRYSA
                     PGKQVKRILERAAELVFRDRLKVPVDVYEGPAMNHSYHEMIIGHGKCLSTVLISDTSE
                     QIPAAGHTADYHRAQFLATQMALAQRGRAVVSIRLRDLEPATLAALDRFFAETAKSLK
                     NGQA"
     gene            5736..6113
                     /locus_tag="VFQ79_05520"
     CDS             5736..6113
                     /locus_tag="VFQ79_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139144.1"
                     /translation="MLWYGAFLAGCGGERGGGAPPDTLGGKWKLASVEPVSADTAPQE
                     VRALGLREARCAVYTGTPDVTVMLYEMTTGPGAFEMVQKWRPRPGAVSFYKDRFFVVC
                     EAAGNNEAELLDFSRLMEASLAE"
     gene            complement(6136..6708)
                     /locus_tag="VFQ79_05525"
     CDS             complement(6136..6708)
                     /locus_tag="VFQ79_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU0139145.1"
                     /translation="MDTNSRSTPETPGSGSLRAVMEHIVERHHTFCRNEVVRLSSLFG
                     EVTAEWGKQHPELKRMGALFAAMAKDLHMHLIKEEQTLFPYIARLEEAVQRGTAVSWP
                     PFGTVENPIRIMIEEHDQTDHELKEMRSLSGGYMFPPDAPASYAALYEGLRSFEREME
                     DHIRLENEELFPRAIAMEQDACARKTAGQG"
     gene            complement(6716..8149)
                     /locus_tag="VFQ79_05530"
     CDS             complement(6716..8149)
                     /locus_tag="VFQ79_05530"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU0139146.1"
                     /translation="MRILKRVWNWFDDITGISAALGPLLKHPVPRARKSAWFYVLGSA
                     TLISFFLQIVTGIALASGYVASSGQAYDTLVFITGTDIGRIIRGMHAFGASAMVILIG
                     LHTIRVYLMGAYKYPRQMNWLTGAGLLLLTLLMAFTGQLLRWDQTGFWSVVIAAEQAG
                     RTPVIGQWLARFIMAGDTVGGATLSRFFAAHVFFIPALIFLFVGFHLYLVVHNGISEP
                     PKAGRPVDPKTYRSWYRALLARDGQPFWPDAAWRDAAFGLLVIVAVAVLAIVVGPPEL
                     GPPPDPTIVEASPRPEWYLLWYYAVLSLIPKWSEDWVIILGPAIFGALLILLPLVAGK
                     GERSPIRRPWSFVIVAFTVFMIAYYGTVGHIAPWSPRMESPPLPAEVVGTTSGPVAEG
                     ARVFHYKGCLYCHLISGYGGIRGPDLTYAGDVLTPAQMTTRIYSGGTNMPSYTGNLTS
                     EELSALIAFLSSRHRQPVFPLPLPRKQ"
     gene            complement(8169..8684)
                     /locus_tag="VFQ79_05535"
     CDS             complement(8169..8684)
                     /locus_tag="VFQ79_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU0139147.1"
                     /translation="MSETMQQQSEIQTGKQQERRQFLNRLTILLGSIGAAVVAIPSIA
                     FLLGLRRTPQIWRAVGKVSAFEIGSTTNVSFQDSSPLPWAGVTAQTAAWLRRVDERNF
                     VAFSMNCTHLGCPVRWLAEANLFMCPCHGGVFYSDGSVASGPPPKPLRRYTVRVNNDE
                     VEILTSPVPIG"
     gene            complement(8681..9385)
                     /gene="narI"
                     /locus_tag="VFQ79_05540"
     CDS             complement(8681..9385)
                     /gene="narI"
                     /locus_tag="VFQ79_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEU0139148.1"
                     /translation="MNAFLFIALPYVALVLAIGVGIYRKLRKPYTYSSLSSEMLENRK
                     LFWGSVPFHYGITLILMAHLLAALFPSAAGWLLGGEIRRLVLELTGIALGIYTLFGLI
                     VLIARRLAPKSLAQSVTSYMDGIVLFVLLVQVASGVGVAVFNRWGGVWYLHTAVPWFW
                     SLVALNPDTSTVDSLPALVKVHFVAGFVLILLFPFSRLVHLVMFPLQYLWRPYQVVVW
                     NDRPAVRTSVRRENAR"
     gene            complement(9404..9982)
                     /locus_tag="VFQ79_05545"
     CDS             complement(9404..9982)
                     /locus_tag="VFQ79_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEU0139149.1"
                     /translation="MALYSLFATVFDYPEAPLQESLGCCMALLPPGASESYAQLAAFE
                     SAVRGKSSGQLQEIYTSAFDLHPDCTPNLGYHLFGDDGRRGLFLAELKDRMEARHIPL
                     GVELPDHISLVLRYLDVADEEDRLCLIGDCLIPALSRIAARFEQSGNPYRYVLQALLS
                     LISYQHEELVISPGAVVHHIAAQREAPFHILE"
     gene            complement(9982..11472)
                     /gene="narH"
                     /locus_tag="VFQ79_05550"
     CDS             complement(9982..11472)
                     /gene="narH"
                     /locus_tag="VFQ79_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEU0139150.1"
                     /translation="MDIRAQVSMVFHLDKCIGCHTCSLACKNLWTDRPGTEYMWWNNV
                     ETKPGTGYPTLYEDQEQYHGGWIVENGELRLKLGGKKSELVNIAYNPRLPVLDDYYHP
                     FTYRYKDLIDAPAGDGQPAARPISMITGGDLNIEAGPNWDDDLSGSPVYAANDPNLAV
                     LSEEDRRRFFELQQLVYFYLPRICNHCLNPSCVAACPSGAIYKRGEDGIVLVSQEKCQ
                     GWRMCISGCPYKKVYFNWNSGKAEKCILCYPRMEIGEAPACMHSCVGKIRYLGVVLYD
                     ADRILETASRPEAELVEAQRDMILDPFEPEVIREALNSGVDRKIIESAQKSPVYRYVK
                     EWRMALPLHPEWRTLPMLFYVPPLLPVTATLDQQGNYEMETDFFSSLQSARLPIRYMA
                     SLFSAGDEAEVIAVYRKLLAVRIYKRAQTVGDISMEAARVALEEAELTPENVEDIYYL
                     TALAGFEERMVVPPFLREQVVELGIDVQTFQQERGAGFLHWPKRGL"
     gene            complement(11484..15098)
                     /locus_tag="VFQ79_05555"
     CDS             complement(11484..15098)
                     /locus_tag="VFQ79_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEU0139151.1"
                     /translation="MSWIKDIVNPELRAWEEFYRNRWQHDKVVRSTHGVNCTGGCSWM
                     VFVKNGIVTWEMQAIDYPKLESGLPLYEPRGCQRGIVYSWYIYSPLRIKYPYLRGVLM
                     DLWETARARHKDPVEAWASIVENEESRGAYQMARGKGGFRRASWDQCLEIVVASMLYT
                     AKKYGPDRVVGFSPIPAMSMISYAAGSRFLQLFGGVMLSFYDLYADFPPASPETWGEK
                     TDVAESADWFNSKYIVVTGSNLNMTRTPDAHFAVEARHHGAKLVVLSPDFSQVSKHAD
                     WWIPVNAGMDGAFWMAVNHVILKEFYAGRQVPYFTDYLKRYSDTPFLIEIEKSADGHV
                     AGRFLRANRIARYRDVENGEWKCLMLDRASGQARMPQGSIGFRWQDRKGQWNLELKDS
                     LDGTEIDPQLSLIDSHDDILQIPFIDFGRERTFHRGVPVRYIESVDGPVAVTTGFDLL
                     MAQYGVPRGLVGDYPAGYDDEEIAYTPAWQEKFSGTGRKTVVQFAREFATTAELTKGK
                     CTIIIGSGVNHWYHNNLHYRTGISALILCGCVGVNGGGLNHYTGQEKITPMASWVTLA
                     MALDWSRPPRLQNGPSFHYVHSDQWRYERAHADYHPPAGRFSREHFIDLQVDAVRMGW
                     LPFYPQFNRNPIQLVRDAEEAGAHTIPEIAQWTVDQLQTGKIRFSVEEPALPENWPRV
                     WLIWRANALHSSAKGHEYYLRHYLGTHSGAIAGEVARESVQEVVWREAATQGKMDLVV
                     DLNFRMDTSALYSDMILPSASWYEKDDLNTTDLHSYFHPLGAAVPPCWESRSDWDIFR
                     ALAEKTSELARTHFPEPFRDLVTTPLLHDTADEIAQPEVRDWSRRECDPIPGKTMPHI
                     TVVERDYVNLAHRFNCLGPGIAEHGVEDHGIDMDVADLYEEFAKTVPSYEWNGRRYPS
                     LVDAVSAVNMVLFFAPEANGEIAYRGFQERERQVGLPLADLGENNRGVRYDFEDLTKQ
                     PRRILTSPCWSGIVNHGRAYSGYVMNVERLVPWRTLTGRQHLYLDHEAYRAFAESCPT
                     FKPRISLENSRSIVTSKPIGKSITLACVTPHGKWHIHSTYYDDLRMLTLSRGVEPFWL
                     NDKDAAEIGVLDNDWVEAYNDNGVIVTRAVVSARIPRGIGIFYHAPERTISFPKSPVR
                     NNYRGGGTNSVTRMRLKPVLMAGGYAQNCYRFNDYGPPATDRDTYVIVHKLAGKPRWD
                     "
     gene            complement(15923..17845)
                     /locus_tag="VFQ79_05560"
     CDS             complement(15923..17845)
                     /locus_tag="VFQ79_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139152.1"
                     /translation="MRAQTALFVNQLRVGWNRMQEFELFGTTNNPAFDIAGQLGIPLT
                     SRRPEDFGPPRVQIDGPEGGFSVFDIQNQIGPRDRGYEVWQITDGVSWQRGRHLLKFG
                     AEFNKRYYTKEQARDPRGLYRFDGSFTGSGLADFLLGYLISATINPVPTVIDMDSLWQ
                     SYYVNDEWKVTSNLTLTLGLRYDYFQRWRQFDDRIVNIGQNGFAVTGFITPATSPFGR
                     SLLSPDRNNLGPRIGFAWRPGWLRETVVRGGYGIYYQQEHPNANATMVEGAQATAGAT
                     ILGNPSGAPNVLWSDPFASAQGASANLASATSIDPLMRDAYIQQWNFTVQRRLPGNVL
                     ADVGYVGSKGTRLSIAFDPGALALNRPIELVDPRTPGLAGIDARRPNQAFPRAVSAVK
                     SIGNSNYHSLQAKLERRMSSGFTFLTAYTWAKSLSGPHDQGALIGNGSFIGTPQDYYN
                     LKNEHSLSGFDLRHRFVQTVLYELPALGGGAFARHLTGGWKFATIITAQSGFPAGIVD
                     GRDTTGTGQGSRADIVVGQSPNLSRDQRTWERWFNADAFTLAEWGRFGTSVRTGAITL
                     PGLINADFSIDKSFRLGERSRLELRSEFFNVFRHFNPEPGSVDRGVRSRTFGAVGGGV
                     QGVATRIIQLGAKFYF"
     gene            complement(17846..19249)
                     /locus_tag="VFQ79_05565"
     CDS             complement(17846..19249)
                     /locus_tag="VFQ79_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139153.1"
                     /translation="MRSFAMRQTLTITVLSILLHVSAAAQQITGSIVGAVTDPSGAVV
                     PNAVLTIVNVNTNASRQTRTDASGNYSVPFLPAGTYRLTSMASGFQQNVVDGVSLQVG
                     QTSRVDVQLQVGDTSQTVEVQGLVAVLQTENPTVGAVLDGQKIVDLPLNGRNFAQLAH
                     LIPGIQPGTPGSITVRRGRGSIGQVDSPFGSTGMSANGIRDTANRYFIDGVESMDGDA
                     FSYAFSPSIDSLAEFKVETSSYSAENGAAPGAQISLITKSGTNTFHGTLWEFNRNDAL
                     TSAYNAIGRVDLKAPRLNRNQFGANIGGPVRLPKLYNGTDRTFFFFNWEAGRQALGAV
                     AGFRIVPTQAQRNGDLSGLVDARTGQPLTLKDPLGVGIVNNRIPQSALSPQAQTILSY
                     TAMPNTQNGIFNFINTPQSPVSKQDNYTARVDHNFSTKDIVSARYVFNDTVESGTPFW
                     GNDERNNLARTQNVSTS"
     gene            19480..20631
                     /locus_tag="VFQ79_05570"
     CDS             19480..20631
                     /locus_tag="VFQ79_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139154.1"
                     /translation="MCQSTAFAGSEVLRNMLSYLSRASAEHPGRPAKEYELGVAVLGR
                     KDGFDPRLDSSVRVHCARLRTRLLEYYLEEGASDPVELTIPKGAYNLVARYRDVPRKP
                     EIQTTVLPTMPAPTGRHPLLWTSWAVSAVAVATAIWFWSLLPPPVSAELKHFWNTFDS
                     RPDETLVVFSNPRFVGSVSNGGLLYEPPNSQVPLKEINDRYTGIGEAMALQQLTRLFE
                     RLRLPLRAKRSGLLTWDEARSRNFIFIGAPEVNRPQMELPKLEQFGFKPADEEPQSGR
                     GGIINLQPQAGEQQYYLNSGPPYTHDYAVIALVPGLSAGRRALILAGRTTYGTQGAVE
                     FLLDQKSTSGLLSKLDAQRSRLPFFEALLRVKVSGGVPVQPELVILRRR"
     gene            20709..21749
                     /locus_tag="VFQ79_05575"
     CDS             20709..21749
                     /locus_tag="VFQ79_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139155.1"
                     /translation="MPTASKKPLKIAVCGLGFMGSTHLKALKNVKEAELVAVVSDVPE
                     KLTGDLSAIQGNLGGPGEKWDFSHLKKYNNVYDAIRDKDIDAVDLCLPTHLHKPVTIE
                     ALRAGKHVLVEKPMALDGAECDEMVEEGEESGRVLMVAQVLRFFPEYVSLRETLRSKT
                     LGEVRAAIFRRRCAGPTWQRWVYDKSRSGGGVFDLVIHDVDFCLQVFGQPEAISAVGY
                     EKLENGVDWLTGTLYYPNIPGGIVIQGGWHHPKTFPFSMEYSVVADGATLEYSSAGRP
                     ATQYNADGEKPIDVKQQDGYEAELRYFAECCVEGKRPELCPPSESSLAVKLCQALVDA
                     RGENGKRLEWKG"
     gene            complement(21788..23455)
                     /locus_tag="VFQ79_05580"
     CDS             complement(21788..23455)
                     /locus_tag="VFQ79_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0139156.1"
                     /translation="MPTGAELFVDAAQRLGINEFFTLVGDHLNEVLRVAADRGVRVID
                     FRHESGAAHAADAWARIHRKPALAIVTGGPGHTNAMTGLATAHLAASPLILVSGAPAA
                     SMAGRQLFQVIDQVGMAAPVAKYAAQPASAGQIPFHLGRAYAESMAGRMGTAHLTIPV
                     DLFVAEAAQPLRMPEPYAAPAAVPADSEIDRAIELIANATRPIVIAGSGVWWAGAEQE
                     FSEFLKHSRLPFYTITMARGIVSDLEPYSMGYADPALNKAVHVAFQEADVVLVLGKRI
                     DYRLALGGPRLFRPEAKFIQVDIHSAELGLNRTLEAAICADLKQTLQAMCDRLGERGW
                     EPREEWIERQRKLVSAWRDDLRNAAGGDSSPMHPAAFYREFERALPEGTLFSWDGGDF
                     AHWGRAILPAVTPGGWLRLGPLGTIGSALPNSIALQMANPARPVVMITGDGSLGFYIA
                     ELDTIVRYNLPIVIVVGNDAGWGLERELQSEFYPDRETVGCELRRTRYDLVMKGFGGE
                     GENIERIEDIRPAVERAFASRRPYLLNVNVQGTRSPFTEWQIAGKKR"
     gene            complement(23457..24575)
                     /gene="dgt"
                     /locus_tag="VFQ79_05585"
     CDS             complement(23457..24575)
                     /gene="dgt"
                     /locus_tag="VFQ79_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEU0139157.1"
                     /translation="MKPTLGHLRFPVSQARGRRYPELLHPYRNEFQRDRDRIVHARAF
                     RRLEDKTQVFPPGISDHFRNRLTHTIEVSQIARTAAAVLDLDEDFTEALALAHDLGHP
                     PFGHAGEQELNRQMRKYGDRFDHNLHALRIVETFEQRYARFPGLNLTFEVREGMAKHS
                     RDFKAGQFPMLDEYLPGRKPPVEAQLIDPADEIAYNTADLDDGYTARLFSVDDVCAAV
                     PEFAEVWNNMEFQFPGAPERVRFSEVVRGLIDTLASGLIEGTSQSARQAGLETVDDVR
                     HHSSRIARLTPAVEATNTALKSFLQTKVYQSDVVLRERQASAGMITALFEAFMKDPEK
                     LPEPYVEQATEDSVHRVVCDYIAGMTDGFLRRTYDQMI"
     gene            complement(24592..25296)
                     /locus_tag="VFQ79_05590"
     CDS             complement(24592..25296)
                     /locus_tag="VFQ79_05590"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0139158.1"
                     /translation="MDRISRKELKSDRFQERVGSTVEYLDEHRRQVILIGGIALAVLL
                     VGWGAYWYSGKQKIVRQQALTQALDIQNSAVGPPSGNPLVKTFPTAEARREEATKAFQ
                     QIINDHSGSEEATIARYYLGVIAAEAGKEADAAKHFQEVADAGRNPYSSLAQFALAQL
                     YQGQGKTAEAEKLLRALIDRPSMLVSKEQATIALAKVIAPKNPAEARKLLEPLRTERS
                     AVSRMALTALSDIPAQ"
     gene            complement(25367..26434)
                     /gene="recA"
                     /locus_tag="VFQ79_05595"
     CDS             complement(25367..26434)
                     /gene="recA"
                     /locus_tag="VFQ79_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU0139159.1"
                     /translation="MDEKERARVLGVTLGQIEKQFGKGSILRLGSREAIVPVSAISTG
                     SISLDAALGVGGFPRGRISEIFGPESSGKTTIALQVIAQAQKNGGVAAFIDVEHALDP
                     GYASRLGVDVDNLLVSQPDYAEQALEITNALIASSSIDVLVLDSVAALVPKAELDGEM
                     GDSFMGVQARLMSQAMRKLTGVVSKSNTALIFINQIREKIGVMFGNPETTTGGRALKF
                     YSSVRADIRRIAAIKDGDTVTGNRTKMKIVKNKLAPPFREAEFDILYGEGVSREGDLI
                     DLGVAQNVVEKSGSWYSYKGERIGQGRENARQFLKDHADTRDQVDAELRKVLGLIRPE
                     TPAPAAPENGPRPAATGARTR"
     gene            complement(26536..27489)
                     /locus_tag="VFQ79_05600"
     CDS             complement(26536..27489)
                     /locus_tag="VFQ79_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0139160.1"
                     /translation="MQFRRRLLTAAVCFFALLAGSLRVAGEVDQKKGTPAPDDPNRII
                     LDVTRVNILFTVADRKGRFVTDLIKDDFLVFEGKNRQEILEFTAESDLPLRLAILIDT
                     SNSIRDRFRFEQEAAVEFIQSLLRPGQDKAVVVSFDTAAELVADLTDDQRQLENAIRD
                     LRPGGGTALYDAIFFACRDKLMLDQPRHKFRRAMVVLSDGDDNQSRYTRDQALEMAQK
                     ADVVIYTISTNITRIPSQGDKVLKYFAEETGGLTFFPFKVQDLSQSFENIANELRHQY
                     NLLYRPEPLRADGQYHEVEVKVKDRKGLLVRARKGYYAPKL"
     gene            complement(27508..28425)
                     /locus_tag="VFQ79_05605"
     CDS             complement(27508..28425)
                     /locus_tag="VFQ79_05605"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU0139161.1"
                     /translation="MRLESTTSGRGKRLDHFLKDHLPQYSRARIQQWIRQGLVLVDGR
                     PGKSSYTLRGGETIDVEPGPLPPLHAEPEDLPLDILYEDQDLVAVNKPAGVVVHAGAG
                     RHSGTLVNALLHRFQNLSAVAGDLRPGIVHRLDRQTSGVLLVARTDFAHRRLAHQFAS
                     REVRKTYLALVHGNVRADAGKVDKPISRDPVRRIRMSVKSGRGRESLTEYRVLERFED
                     FTLLEVRIATGRTHQIRVHMAAIGHPVAGDRLYGAPRSEPPTERFFLHAQRISFHSPS
                     TGAEITLEAPLPEELKLWIERLKNRSGPI"
     gene            28466..29374
                     /locus_tag="VFQ79_05610"
     CDS             28466..29374
                     /locus_tag="VFQ79_05610"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEU0139162.1"
                     /translation="MIRSAIVPVAGYGTRLLPATKSQPKEMLPVARKPIVQYVAEELA
                     ANGVRQILFITGRNKSSIENHFDSDPELLRILKAANKRDLLQELGFEDLDVKYLYTRQ
                     RQQMGLGDAVLCGENFAGEEPFVVALGDSILGLHAQSRSVSRMADLFESRRASCVIAV
                     EEVPIEETRHYGIVQAGDGEGEAGAYRVLNLVEKPRPEEAPSNLAIAGRYIFSPLIFD
                     MIRRIAPDARGEIQLTDAIRMMCEEGKRVLAVKLPPGEKRYDIGNFPSYFETFVEFAL
                     ADPVYGAGFRASLERLLEDKARQALK"
     gene            29371..30783
                     /locus_tag="VFQ79_05615"
     CDS             29371..30783
                     /locus_tag="VFQ79_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139163.1"
                     /translation="MTTRAVFALIAVVLVVSRLCHSQVLWAEEGLPLAAALQMISGKF
                     LYSEIWFDKPPLTTAIYVLWGAHTGWLLRLAGALYALLACVLAYLIARDLWSAREGRI
                     AAALLAFFLTFGIQSAVIPLAADLLMLVPHLAAVWFAWQRRALWSGLAAGVALLTNAK
                     AAFVILFCGVLLYRYLVRFSLGVFIPNAAVLLWLQQAGALDDYILQVWELGRLYSAHT
                     FVTEPWKEGAIRTLNWAGFHAALILPAAWFWRKEAGSQRALFALWAALSLAGVFTGWR
                     FFPRYYFQLLPVFTLAAARGTAGMGRMRWLAVLALAIPFFRFAPSYVSLGASALAGQP
                     SGWRDVALDRDSQDASRILMRETASGDTLFVWGFRPAIYAYTRMPAASRFLESQPLTG
                     VLADRHLRRTDALSAGWIQTNRKELTESRPAFIVDGLGLYNPGLGITRFRDLDLWLSN
                     YEIAGKTRGTIIYRRKPASK"
     gene            complement(30837..31619)
                     /locus_tag="VFQ79_05620"
     CDS             complement(30837..31619)
                     /locus_tag="VFQ79_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0139164.1"
                     /translation="MASPKLFGVSLGALLLLGVTGTGCATKKHVAKVVAPVEQRVGEV
                     EKQNQDQQARLGELGNEVSRADERAMDAEKKASAAGQAAAQAGDAAGQARQRADGAYG
                     LAEQTQTRLGEVQQNVDRTFGNLDNYRLVTEDKVYFGFDRSVLTKEAKADLDSIAGSF
                     GNMQNFVVEIQGYTDHTGSSNYNLELSQKRASAVVRYLATEHNVPLRRISVLGVGEAP
                     TPGKTRAERQENRRVDVKVYALDLKGEGVNMESRTKPPAQQQ"
     gene            31901..32179
                     /gene="rpsT"
                     /locus_tag="VFQ79_05625"
     CDS             31901..32179
                     /gene="rpsT"
                     /locus_tag="VFQ79_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU0139165.1"
                     /translation="MANHISALKRMRVERRRTKINRARKTRLRHQVRAMRRLLNDKNG
                     EGAQAALPATYSIVDRAAKWGVIKKNTAARYKARLTGRLKAVLAASAQ"
     gene            complement(32181..33251)
                     /gene="holA"
                     /locus_tag="VFQ79_05630"
     CDS             complement(32181..33251)
                     /gene="holA"
                     /locus_tag="VFQ79_05630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU0139166.1"
                     /translation="MTPQQFISHVQRSHPAPAYLFVGPEHYQRTACRQALLAAMLGPG
                     EENGFVRHDLDEVSLAEVLDDARSLSLFATRRVIWVASAEVALPRTRASAEEDGSERS
                     PSKTGPKALLETYMQSPSGDTVLVFDAARSGSGSEEKSMLDRVRKFYAAVPNHVEFER
                     FSLDSARQTAESLARQADLKIASPELALLVETLDADVARIAVEIEKLRLHTGGTRPVN
                     REDIFLMVPDARAANIFALVASLGRGDREQSLTLLDFLVREGEYLPLVLTFLATQFRL
                     ALVAKEVRLNSASQIQGHFSRQGVQMWRSRAEQVAQTAAAFSKERLETLIRRIYETDK
                     ALRDANPDDRIVMERFVLELAG"
     gene            complement(33255..33770)
                     /gene="lptE"
                     /locus_tag="VFQ79_05635"
     CDS             complement(33255..33770)
                     /gene="lptE"
                     /locus_tag="VFQ79_05635"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEU0139167.1"
                     /translation="MKNAAALAAVLLASAGCGYRITGRSEVLPPTTRVIAIPAFQNLT
                     SRPKLADRIASALTREFITRTKYNVSSDPDTADVIMDGAVTNYASYPEVFDPITGRAS
                     GVQIIVTLHISLRERTTGKILFARPGLVVRERYEISVSELSYFEESDTAGERLSRDVA
                     RGIVSAILTSF"
     gene            complement(33767..34783)
                     /locus_tag="VFQ79_05640"
     CDS             complement(33767..34783)
                     /locus_tag="VFQ79_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEU0139168.1"
                     /translation="MSKSVAIVGSESLLGRELREVLSDKWANQTVTLIGAEETGGILT
                     QDAKEAAFIAPLEREHLMDAGLAFMAGPGTATWKVLDLLSDTGRTPVLVDLSFALEDR
                     PEARLRAPMIEPGGYDVAATAIHVIAHPAAITIALFLDRLHGIEPVARSVIQIFEPAS
                     ERGRRGLDELHQQTVNLFSFKPMPKEVFDAQLGFNMLPRYGTEAAEKLQDVEVRIDRH
                     LATLLGIASHAPLPSLRLVQAPVFHGYSISAWVEFATPPSIARFRDALASPRVEVRDD
                     GLEPPSNVGAAGQSGITLGAIEADRNNPRACWFWVVADNLRISAENAVMVARDLLARG
                     GGSR"
     gene            complement(34776..35660)
                     /gene="pssA"
                     /locus_tag="VFQ79_05645"
     CDS             complement(34776..35660)
                     /gene="pssA"
                     /locus_tag="VFQ79_05645"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEU0139169.1"
                     /translation="MRFPLQDRLQVSGPGGRQPRRAAYALPTLFTAGNIFLGFLSILR
                     SFEGSLLPWGENRHFEVAALAIGVAFALDGLDGRIARMTNTVSDFGREMDSLADVITF
                     GIAPAVLAFTWGFQYIDLPQDGFIRNNLFNLAYFFSFLFLLCGSVRLARFNVQTNPVP
                     RNPGRPDRKYFVGLPIPSAAAMVGAVVYASSGVPITSWPLGIAWICLLALLSFLMVST
                     WRYRSFKDLNLMRPRSPLSIVLLGATIYMIWNFSKPVLVTMAAIYVASGIVVWIGGVL
                     RRLRKGHVPAHGMESADV"
     gene            complement(35657..36271)
                     /locus_tag="VFQ79_05650"
     CDS             complement(35657..36271)
                     /locus_tag="VFQ79_05650"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEU0139170.1"
                     /translation="MIVADGIYYAIGLTAGGLIVSWIGRPLYGLPFYVLAVFCLYFFR
                     DPDRQIPEGDVAVSPADGKVVAVLAQGAGAYRISIFLNVFDVHVNRAPIAGAITQVKY
                     QQGRFAVASKEIASSENEMNTVTVEGGGSTVVFKQIAGLIARRIVFNKKPGDKVAKGE
                     RVGMIKFGSRTDVIFGSEWEIAVREGDRVQAGSSIIARRRDSAR"
     gene            complement(36313..>37260)
                     /locus_tag="VFQ79_05655"
     CDS             complement(36313..>37260)
                     /locus_tag="VFQ79_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0139171.1"
                     /translation="RIEFRLPGRRKTKQLLLLGHSDTVWPLGTLARMPFRERDGRLWG
                     PGVLDMKSGIAFAIQAARALRELDIPVSRRVVLLLNADEEVGSDSSRPLTEREARRSA
                     AVLVLEPGTGLEGKLKTARKGIGGFKVSVAGRASHAGVDFAAGASAIVELARQIAVIA
                     GFTDLDRGITVNPGVVVGGSRSNVVADEASADVDFRFVRQKDGAGLERKFRSLRPIDK
                     RCRLTVEGGVNRPPMERSRAGLELFRHARDLGRTVGLDIAESSTGGGSDGNFTAALGI
                     PTLDGLGGVGEGAHALNESILTDRIADRTAMIAALLSVL"
BASE COUNT         7544 a        10945 c        11444 g         7327 t
ORIGIN      
        1 aggagaatat gagtgcacct tcccgacgat ccattttggg cggagcaatc gcgggatccg
       61 cgttatcagc cgtcgcggcg cgcccgtccg ttggtttccc cgtagagcga gtgtttgcgg
      121 ggctgacgct gatccgaggg ccggtcaact cggcgatctt cgaacgcaat ggtaagctcc
      181 tcgtcatcga ttcaggctct ctgggctcgc gggaactagg cggagtgcca cagtggctgc
      241 tttttacgca tcatcatcgc gatcaggcga tgggggcaga taagttagtc gtggccggcg
      301 cccgcgcgat tgctcccgat gccgaagctc atttgttcga gcgcgccccg gaagtgtggg
      361 agaaggccga ttccgtcatc tatcaccggt ataaattccg ccctcatctg ttcactctct
      421 acaacgccgt ccccctggcg aagagagtga gggatcgcga cgtacatgaa tgggagggcc
      481 tgcgcttcga ggtcatcgac acgccgggcc agacagatgg ttccgtgacc taccttgtgg
      541 agacggaggg atgccggatc gcctttaccg gggatctgat ttacggtccc ggacagatcc
      601 agaacatgta cagtctccag aagacatggc cgggaatgcc gggaggatat tggggcttcg
      661 gaggggcagt gccggacctg aaagccagcc tgcggagagt gctggatcgc agcccggaca
      721 tgctgattcc atcacatggc aagccgatgc gggatccccg gaaagccgtt gaggaattga
      781 accacaatct ggacatggta atgaaaaatt acctcactac gacggcgtgg cgaacctttt
      841 tcaaacaggt actgccgccc gaagcagagc cccggatgct gcagcccatg agcaagcccg
      901 actatccgtc ctggtttcgc gatatcgccg tgacgacgaa ggcaattgtg gccggggaca
      961 agtcggtctt cttattcgac tgcggctttt cagcgcccgt ttcgaaactc gtggaactac
     1021 agaaaaacgg agagatcggc cgaatcgaaa agctatggat cacgcactat catgacgacc
     1081 atgtggactg tgtgaaccgc atcaaggaaa ggttcggcgc cgagttgtat gtgcaggaag
     1141 agatggccga tatcatcgaa catccggaag cgtataacat gccgtgcctc tcacgtgaac
     1201 cagcgcgtgt cgatcgagtt ctccgggatg gcgaggtgtt cgagtggaaa ggttttcgca
     1261 tgacgcctta ctactttccc ggtcagaccc tttaccatgc tgcgctgctt gtcgaaaaag
     1321 gcggagttcg cctgctgttg actggagact cgttcggaaa ctggggaatc gacgattact
     1381 gtagcgaaaa ccggtgtttc gttggaagcg ggcgcggttt tgaaaagtgt ctggaactgc
     1441 tcctgcgcct caagcccgac atgctggtga acgcccattc cggcgccatt cgcttttccg
     1501 aggattacgt tttaaaaact ctcgaggttt ttcgacagcg ggagatgctt tatcgtaagc
     1561 tcctgccctg ggataacccc aatttcggtc tcgatccttt ctggttacga gcatacccct
     1621 ataaacagtc cgcactgccc ggctcaagag tagagcttga ggtccgggtt ctgaaccaca
     1681 gcgaccagac caggatggtg caggcttcac tcgtgttacc cgatgggtgg cgtcccgtcg
     1741 tggtatccgg aacatctcgt ctggcggcag gacaggaagg gcgaatcagg ttaagcgcga
     1801 ttgcgcccga gacacggaac ccaaggcgtt acgtcctggg gctttccgct gtgttcaacg
     1861 ggactcctgt tggtgaggtc gctgagtcga tcgtagatct gctgaactga ggtaatcgcc
     1921 gggacacctc atgagttcga agcatgggaa cacgtgtggc gtcgcgatgg cgcctccgct
     1981 tgcttacgcg cgcggctcgg taaggtgtgc gcgggtcggt gttgctcgct gatcggtgat
     2041 gtttactggt gcgcatttgc ccgcgcaccg ggaccggtcc ctgtcccccg cgcaaaggtg
     2101 tagggggact taggcaggcc tgtcacgtcg acgcgccgcg gtctcaggtt ttacactccc
     2161 cggggtagtc ctgcgcgggt ctcggtgggt tgcctctcgg ccaacgagca actcctcgct
     2221 cataacatac accggcgttc gaagcggcgg tgtgcttgga cgtgcggaga tgcctgaaaa
     2281 gaaacggtga aacgcgcagt ggagtgtatg tgaacgccga tcagcccgcg agtggggcct
     2341 cggtgtacca atcgagcatg gcgcgcagag cctggctgcg atggctgaca cgcatcttct
     2401 gctcgtccgt cacctcgccg aacgtgcgcc cgaacggcgg gtacagaaaa agcggatcat
     2461 agccaaaacc gttgctgccg cgtggagcct ccaggatgcg gccctccacc gcgccacgga
     2521 agagacggag caactcgccc gctcgggcaa cggcgatcac gcagacgaaa cgcgcatgcc
     2581 ggccggttac accctgcagc ctttcgagga ggagccggtt gttctcttca tccgtcgcgt
     2641 tctcgcccgc aaaacgcgcc gagagcacgc cggggtcgcc gccgagggct tccacctcca
     2701 ggccggaatc gtcggtgaat accgggcctg gggcgaaccg gctgtaatag gccgccttct
     2761 ccgccgcatt ctgttcgaag ctcaacgccg cctcttcaaa aggcgcgatc cgctccaggc
     2821 cgggaacggg ttcgatcccg atgccgcgct gctcagcggc tatccggaac tcgcgaagct
     2881 tcccgggatt ggtggtggcg caatagagtt tcaagcggac tccagcacct tcttctgagc
     2941 ggcggtaagc tgctgaattc cgccgcgcgc gaggtcgatg agctcgagca gacgaccgtc
     3001 gtcgaacggt gtctgttcgg cggtagcctg aagttctaca aaccggcccg ctccggtcat
     3061 gaccacattc atgtcgactt ccgcccggga gtcctcttca tagcaaagat cgagcagcag
     3121 ttctccctgg aggattccga cgctggtagc ggcaaccgaa tcgcgaatcg gggatttctt
     3181 caggacgccg aactgcaaca gttgccggat ggcgagcgag agcgagacga aggccccggt
     3241 gatggccgcc gttcgcgtac ctccatcggc ctggagcacg tcgcaatcga ggataacggt
     3301 ccgttcgccc aaggccaccg gatcaacaat cgagcggagc gatctgccaa tgaggcgctg
     3361 gatttccagc gtgcggccgg acggtctgcc gcggttgact tcgcgcgccg tccgggtggc
     3421 cgttgcccgc gggagcatcg agtactccgc cgtgacccaa ccctttccgg tgccgcgcag
     3481 aaacgaagga accgagtctt cgacactggc cgcgcaaagc acccgggtgt tgccaatttc
     3541 gatgatcgca gacccctcgg ccgtcaacaa ataacccgtc gtgatggtga ccggtctcag
     3601 ctcggcgggt tgccggttat cgattcgcat aacgcagttt catctcgggc tcagcacgga
     3661 aacgaacagg agatacatga gcagcatggc gccgaggagc accagcaggc acggaatggc
     3721 gccgcgagca gaagaagcgc tctttttctt ttgccctttg gccggcttga atctggccat
     3781 aagaaattga tcgtaccatc aggcttgccc attcttaagc gatttggcgg tttcggcgaa
     3841 aaaacggtcc agcgccgcaa gcgtggcggg ctcgaggtcg cggaggcgga tggagaccac
     3901 ggcgcgcccg cgttgcgcca aggccatctg cgtagcgaga aattgcgcac ggtgataatc
     3961 cgccgtatgc ccggcggcgg gaatctgttc ggaggtgtcc gagatcagca ccgtcgaaag
     4021 gcactttccg tgtcctatga tcatctcgtg ataggagtgg ttcatcgccg gaccctcata
     4081 cacgtccacc ggcactttca agcggtcgcg gaatactaat tcagccgccc gttccagaat
     4141 tcgcttcacc tgctttcccg gcgccgagta tctcgtgtcg ccgaaaaagg tgagctcgcc
     4201 gtattcggtt tcgcgcgggc agaagagctt gcgcagaatg cccgcgtata cggccggcgc
     4261 atcgcggccg gccggatcca ccggcaaagc aaggcggtcc caggcgaatg taatgccacc
     4321 gcgccacttc agttgacagg aggaattctg catcgcctgc cattcgcgcg tgctctccac
     4381 agctccggct ttcaccgcgt cgccgtgcaa gcggccggtg atggacttgt acaactcaac
     4441 gctgggctga gtgacgaagt tcatcccccg aaggtacgcg actccgaaga ccacgtagtg
     4501 aataaactgc atgtagtgcg agagcagggt tccgcgcggc gcgaccaatg tggcaagagg
     4561 atagccggcg cggcgcagca tggtagtctt ctgcgaatcg ggaggagtga gccctttgac
     4621 ggagagcgcg agaaagacgc gatcctggcg gccgagcttg ggtgaaacat aattggccag
     4681 ttttggtttt tcgccgatga cgattttcag tccgagatcc tcgctcttgc cgaggctttc
     4741 ttcaaagttc tgcttggtcc acaaggccgc gccgctccat tccctgggaa gcagcaaggc
     4801 caccttgtcc cttccggctt tcccctgttc atgcagaaag gaagcaagcc gccaggcggt
     4861 atcgatctcc tcctgggtga gcagggtggc atcgatccag agccgcagat cgtttttggc
     4921 caatccgagc ggataaaggc tgccgcgggt gagcggagcg ctatggcggc cggcagtggt
     4981 gttgccgcca tcgagttgca actcgactct gcggtagccg cgcggcccgg caaactggtc
     5041 cagaagagag ccggtaagcg tcaagtacac gaagttcggc cggctatcga tcttgtgccg
     5101 cttataaaga cggtacagcc tttcgagatt taccaccggc tcataggagg tcatgcccat
     5161 ggccataccc accacgagga cctggggcag aactttctcg agcggcaggc gcgagcggtg
     5221 cacaatgtcc gcgagcacgg ctttcatttt ggccgggtcc gtcgaatcga gcacatagag
     5281 gcgcggcccc cgccggagca ggccaacggt ctgatacatc gatttgtctt ccgctgagcc
     5341 gcccattccc gcccagatca cgtagcgcag cggcacgccg tgggtgtccg cgatacggcc
     5401 gcggatctcc tccaattcgc ggagcacctg gtccggccat tccggatgtt cctgcgcccg
     5461 ccaggccagg ttgaacacac catagctgtt cctgatgatg cgcgcggagc catccgtctc
     5521 cattccaatg cgaatcagcg tgttcctggc ggggtcgaga tccccaagac ccttgttttg
     5581 aatgcgcgag tccagcgcct tcagccgttc gaatacgttc ttctcctgaa aaatttgaaa
     5641 agcggattga gaggaaggat tgtcgtgata taaggcgaga agcacctacc agatgtagca
     5701 tatgagacaa cagcatgaat cgaagagaat gtttgatgtt atggtatggt gccttcctgg
     5761 cgggatgcgg cggagaacgc ggcggcggcg caccgccgga cacgctcggg ggcaagtgga
     5821 agctggcgtc agtcgagccg gtaagcgcgg atactgcgcc gcaggaggtg cgcgcgctcg
     5881 gactgcgcga ggcccggtgc gccgtgtaca cggggactcc cgatgttacg gtgatgctct
     5941 atgaaatgac cactggcccc ggagctttcg agatggtgca gaaatggcgg ccgcggccgg
     6001 gcgccgtttc cttttacaag gaccgctttt tcgtggtgtg cgaagcggcc ggcaacaatg
     6061 aagcggaatt gctcgacttc tcgcgattga tggaggcgag cctggcggaa tagagcagcc
     6121 gcctacactc gcgggctagc cttgacccgc tgttttgcgg gcacacgcat cctgttccat
     6181 agcgattgcg cgcggaaaca gttcctcgtt ctcaagccgg atatgatctt ccatttcacg
     6241 ctcgaaagag cgaagtccct catagagcgc ggcatagctg gcaggtgcgt ccggtgggaa
     6301 catataacca ccgctcaggc tgcgcatctc cttaagctca tggtccgttt gatcgtgctc
     6361 ctcaatcatt atccggatgg ggttttccac ggtgccgaaa ggcggccatg acacagcggt
     6421 cccccgctga acagcctctt ccaggcgagc aatgtaagga aacagcgtct gctcttcctt
     6481 aataaggtgc atgtgcaggt ctttcgccat cgcggcaaac agcgctccca tgcgtttcag
     6541 ttccggatgt tgcttgcccc actccgccgt tacttcgccg aagagggacg aaagccgcac
     6601 aacctcattc cggcaaaacg tgtggtgtct ctctacgatg tgctccatga ctgccctgag
     6661 cgagccgctt cccggcgttt cgggtgtact gcgcgagttc gtatccattt gtctcctatt
     6721 gtttgcgggg caggggtaaa gggaacaccg gctgacggtg ccgggaactg agaaaggcaa
     6781 tgagagcgga cagctcttca gaagtcaaat tcccggtata agagggcata ttggtgccgc
     6841 cgctgtaaat tcgtgtcgtc atctgcgcgg gcgtcaagac atcaccggca taggtcaggt
     6901 ccgggccgcg aattccgcca tagccggaaa tgaggtgaca gtagagacac cccttatagt
     6961 gaaagacccg cgcgccctcg gcaaccggtc cgcttgtggt gccgaccact tccgctggaa
     7021 gcggcggcga ttccatgcgg ggcgaccatg gcgcgatgtg ccccacggtt ccgtaatagg
     7081 caatcatgaa aactgtgaac gctacaatca caaacgacca gggtcgccgg atggggcttc
     7141 gttccccttt tccggcgacc aaaggaagca gaatgagaag ggctccgaag atggcaggcc
     7201 cgagaataat aacccagtct tcagaccatt tgggaatcaa cgagagcacg gcatagtacc
     7261 agagcagata ccactcgggc ctgggagaag cttcgacgat agtagggtcg ggcggcggtc
     7321 ccaattcggg ggggccaacg acgatcgcga gcaccgcgac cgctacaatg accagcagtc
     7381 cgaaggcggc atcgcgccag gcggcatcgg gccagaaggg ctggccgtct cgcgcgagca
     7441 gcgcccggta ccaggagcgg taggttttgg gatccacggg gcggccggcc ttgggaggtt
     7501 ccgagatccc gttatgaacg acgagataga gatgaaaacc tacgaacagg aaaatgagcg
     7561 ccgggataaa gaatacatgg gcggcaaaga agcggcttag cgtggcgccc ccgactgtat
     7621 cgccggccat gataaaacgc gccagccatt gaccgattac cggcgtcctt cccgcttgct
     7681 cggctgcaat caccactgac cagaagccgg tctgatccca gcgcagaagc tggccggtga
     7741 acgccatcaa tagcgtcagc agcagcagtc ccgcaccagt cagccagttc atctgccgcg
     7801 gatacttgta ggcccccatc aaataaacgc ggatggtatg caggccgata agaattacca
     7861 tcgcggaggc gccgaaggca tgcatacccc gtatgattcg cccgatatcc gtccctgtga
     7921 tgaagacgag cgtgtcgtag gcctgcccgg aggaagcaac atagccgcta gccagtgcga
     7981 tgccggtgac gatctggagg aaaaacgaaa tcagggttgc actgcccaga acatagaacc
     8041 atgccgactt tcgcgcgcgc ggtaccggat gcttgagaag cggccccagc gcggctgaaa
     8101 tccctgtgat gtcgtcgaac cagttccaca cccgcttgag aattcgcata tccttcgccg
     8161 cgctccaatt acccaatagg aaccgggctc gtcagtatct ccacctcatc gttgttaacg
     8221 cggaccgtgt accggcgcaa aggtttgggc ggagggccgg aagcaacgga gccgtcggaa
     8281 tagaagactc ctccatgaca agggcacatg aacaggttcg cttccgccag ccagcggacc
     8341 ggacagccga gatgagtgca gttcatggag aacgcgacaa agttccgttc gtcgacgcgg
     8401 cgcagccagg cggcggtctg ggcggtgact ccggcccagg gcaggggcga cgaatcctgg
     8461 aacgagacat tcgttgtcga tccaatctca aacgcgctca ccttgcccac ggcgcgccag
     8521 atctgagggg ttctgcgcaa accgagcagg aaggcaatgc tcggtatggc aaccaccgcc
     8581 gcgccgatgg acccgagaag aatggtgagg cggttgagaa attgccggcg ctcctgctgc
     8641 ttgccggtct ggatttcgct ctgctgttgc atggtttcac tcaacgggcg ttctcccttc
     8701 gaacagacgt tcgcactgcc ggccgatcgt tccagacgac cacctggtaa ggcctccaca
     8761 ggtattgcag cggaaacatg accaggtgaa ccaggcggct gaaggggaat aaaagaataa
     8821 gcacgaagcc ggcaacgaaa tgaactttca ccaacgccgg aagcgaatcc acggtgctcg
     8881 tatcggggtt tagcgcgacc agggaccaga accacggaac agcggtgtgc aagtaccaga
     8941 cgccgcccca gcggttgaat accgcgacgc cgaccccgga cgccacttgc accagaagta
     9001 caaaaagaac aatgccgtcc atgtacgatg tgaccgattg cgccagcgat ttcggagcca
     9061 ggcgtcttgc gatcagcacg atcaatccga aaagggtata aatgcccaaa gctataccgg
     9121 tcaactccag caccaggcgg cgaatctcgc cgcctaaaag ccacccggcg gccgacggaa
     9181 acagggccgc cagcaagtga gccatcagaa tgagcgtgat gccgtagtgg aaaggcaccg
     9241 aaccccagaa cagtttccgg ttctccagca tctccgagga aagactcgag taggtataag
     9301 gctttcgcag tttgcgataa atgccaacgc ctatcgccag aactagcgcc acgtagggga
     9361 gagcgatgaa aaggaaagcg ttcatagcat tctctgaaac aaatcactcc aggatatgga
     9421 agggagcctc gcgctgggcc gcgatatggt gtaccacggc tccgggagag atcacgagtt
     9481 cttcatgctg gtacgaaatc agactcaata gagcttgcaa gacatagcgg tacggattac
     9541 cggactgttc gaaccgggcg gcgatccgcg aaagcgccgg aatcagacaa tccccaatga
     9601 ggcacaaccg gtcttcttca tcggctacgt ccaggtatcg aagcacgagg gagatgtgat
     9661 cgggcaattc cacgcccagc gggatgtggc gagcctccat ccggtctttg agttccgcca
     9721 ggaagagtcc ccttcgtccg tcgtcgccga acaggtgata tcccagattg ggtgtgcaat
     9781 cggggtgcag atcgaaggcg ctcgtataga tctcttgcag ttgcccggaa ctcttgccgc
     9841 gaacggcgct ttcgaaagcc gcgagttgag cgtacgattc agacgcgccg ggagggagta
     9901 gggccatgca acagcccagg gattcctgga ggggtgcttc cggatagtcg aaaacggttg
     9961 caaagagcga ataaagcgcc atcacaatcc tctcttgggc cagtgaagaa acccggcgcc
    10021 gcgctcctgc tgaaacgtct ggacgtcaat cccgagttcg accacctgtt cgcgcagaaa
    10081 cggaggaact accattcgct cctcgaaacc ggcgagagcg gtcagatagt aaatatcttc
    10141 cacattttcg ggggtcaatt ccgcttcttc cagcgcgacc cgcgccgcct ccattgagat
    10201 gtccccgacg gtttgcgcgc gcttatagat gcggaccgcc agcagtttgc gataaaccgc
    10261 aatcacctcc gcctcatcgc cggccgaaaa caggctcgcc atatagcgaa taggcagccg
    10321 cgcgctctga agcgagctga agaaatccgt ttccatttca taattgccct gctgatcgag
    10381 cgtggcggtt accggcagca gaggcggcac atagaacagc attggaaggg ttcgccattc
    10441 cggatggagc gggagcgcca ttctccactc tttcacgtag cggtaaaccg gcgacttctg
    10501 agcggactcg ataatcttcc ggtcgacacc gctgttcagg gcttcccgga tcacttccgg
    10561 ttcgaaggga tcgaggatca tatcgcgctg tgcttccacc agttcggctt caggtctgga
    10621 ggcggtttcg aggatacgat ccgcgtcgta gagaacgact ccgagatagc ggatctttcc
    10681 gacacacgaa tgcatgcatg cgggggcctc gccgatttcc atgcgcggat agcaaaggat
    10741 gcatttttcc gctttgccgc tattccagtt gaaatacacc tttttataag gacagccgga
    10801 aatgcacatg cgccagccct gacatttctc ctggctgacc agaacgatac catcctctcc
    10861 gcgtttgtag atggctccgg agggacaggc cgcgacacaa ctcgggttca ggcagtgatt
    10921 gcagatgcgc ggcaagtaga aatagacgag ttgctggagt tcgaaaaacc gccgccggtc
    10981 ctcttcgctc agcacagcca ggttagggtc gttggccgcg taaaccggcg atccgctcag
    11041 atcgtcgtcc cagttcgggc cggcctcgat attcaagtcg ccgccggtga tcattgaaat
    11101 cgggcgggcg gcaggctggc cgtcgccggc aggcgcatcg atcaggtctt tatagcggta
    11161 ggtgaacgga tgatagtagt cgtcgaggac gggcaaccgc gggttatacg cgatgttgac
    11221 caactcgctc tttttgccgc cgagcttcag gcgaagctct ccattttcaa cgatccaccc
    11281 gccgtgatac tgctcctggt cctcgtataa ggtcggatag cccgttcccg gcttggtctc
    11341 gacgttgttc caccacatgt actcggtgcc tggacggtcg gtccaaaggt tcttgcaggc
    11401 aagactgcac gtgtgacatc cgatgcactt gtcgaggtgg aacaccatcg agacctgagc
    11461 gcgtatatcc atcgggttcc tccctaatcc cagcgtggtt tgccggcgag tttatgaacg
    11521 atcacgtacg tatcgcggtc cgtggccggc gggccgtaat cgttgaagcg gtaacaattc
    11581 tgcgcgtacc ctccggccat cagtaccggt ttgagccgca ttcgggtgac actgttggtt
    11641 ccgcccccgc ggtaattatt gcggaccggc gattttggaa atgagatggt gcgctcaggg
    11701 gcgtgataga agatgccgat gccgcggggg atgcgcgcgc tcactacagc gcgggtgaca
    11761 atgacgccat tgtcattgta ggcctccacc cagtcgttat cgagaacgcc gatctcggcg
    11821 gcatctttgt cgttgagcca aaagggttcg acgccgcgcg acagcgtcag catgcgcaga
    11881 tcgtcatagt aggtggaatg gatgtgccac ttgccgtggg gcgtgacgca ggccagtgtg
    11941 atggattttc caatgggctt gctggtgacg atacttcgcg aattctcgag gctgattcga
    12001 ggcttgaaag tcggacagga ctccgcgaag gcgcgatacg cttcgtgatc cagatacaag
    12061 tgctggcgtc cggtgagggt tcgccagggc acgaggcgct cgacgttcat gacgtagccg
    12121 ctgtaggcac ggccgtggtt aacaattccc gaccagcatg gactggtcag gatgcggcga
    12181 ggctgcttcg tcaagtcttc gaagtcgtag cgcacgccgc ggttattttc tcccaaatcc
    12241 gccagcggca ggccgacctg gcgttcccgt tcctggaagc ctcgataggc gatctcgccg
    12301 ttcgcttcgg gcgcgaagaa gagcaccatg tttacggcac tcacagcatc caccagggag
    12361 ggatagcgcc gtccgttcca ttcataagac ggaacggtct tcgcgaattc ctcatagagg
    12421 tcggcgacat ccatgtcgat tccgtgatct tccacaccgt gttcggcgat gccgggaccg
    12481 aggcaattga agcggtgcgc caggttgacg taatcgcgtt caacgaccgt aatatggggc
    12541 atcgtcttgc cgggtatggg gtcgcattcg cgcctgctcc aatcgcgcac ctccggctgc
    12601 gcgatctcat cggcggtatc gtgcagcagc ggagttgtga ccagatcccg gaacggttcc
    12661 ggaaagtggg tgcgcgcgag ttcgcttgtc ttttccgcaa gagcgcggaa aatatcccag
    12721 tcgcttctgg attcccagca cggcggaacc gccgccccca gggggtgaaa gtaggaatgg
    12781 aggtcggtgg tgttgagatc gtccttttcg taccagcttg ccgagggcag aatcatgtcg
    12841 gagtagagtg ccgacgtatc catgcggaag ttgagatcga cgacgaggtc catcttcccc
    12901 tgcgtggcag cctcgcgcca gacgacctcc tggaccgatt cccgagcgac ctcaccggcg
    12961 attgcgccgg agtgagttcc gagataatga cgaagatagt attcatggcc cttagcgctg
    13021 ctgtgtagtg cgttggcgcg ccagatgagc cacacgcggg gccagttttc gggaagtgcg
    13081 ggctcttcca ccgaaaagcg gatttttccg gtttgaagct gatccacggt ccactgcgcg
    13141 atctccggaa tggtgtgtgc gccggcttct tccgcgtcgc gtacaagctg tatcgggttg
    13201 cggttgaact gcggataaaa gggaagccag cccatgcgca cggcatccac ctgcagatct
    13261 atgaagtgtt cgcgtgaaaa gcggccggcg ggcggatgat aatcggcatg ggcgcgctca
    13321 tagcgccact ggtcgctgtg gacatagtga aacgagggcc cgttttgcag tcttggcgga
    13381 cggctccaat cgagagccat ggccagagtg acccaggagg ccatgggtgt gatcttttcc
    13441 tgtccggtgt agtgattcaa cccaccgcca ttcaccccga cgcatccgca caagatcaac
    13501 gccgagatcc ccgtacggta atgcaaattg ttgtggtacc aatgattgac accggagccg
    13561 ataatgatgg tgcacttgcc cttcgtcaat tcggcggtcg tggcaaattc acgggcgaac
    13621 tgaacgacgg tcttgcggcc ggtaccgctg aatttttcct gccaggctgg cgtgtaggcg
    13681 atttcctcat cgtcgtagcc ggccgggtaa tcgccgacga gcccgcgcgg cacaccgtat
    13741 tgagccatca gcaggtcgaa accggtcgtg accgcgacgg gaccgtccac cgattcgata
    13801 tagcggaccg ggacaccgcg gtggaacgtt cgctcgcggc cgaaatcgat aaacgggatc
    13861 tgcaggatgt cgtcatggct atcgatcagg ctcaattggg gatcaatttc ggtgccgtcg
    13921 aggctatctt tcagttcgag attccactgc cccttgcgat cctgccagcg aaagcctatg
    13981 gaaccctgcg gcatgcgcgc ctgcccgctg gcgcgatcga gcatgaggca tttccattcg
    14041 ccgttctcca catcgcgata acgggcaatg cgattggcgc gcagaaacct tccagcaacg
    14101 tggccgtccg cgcttttctc gatctcgatc agaaacggag tatcggagta acgcttcagg
    14161 taatcggtaa aatacggaac ttgccggccg gcgtagaatt ctttgaggat gacatggttg
    14221 acggccatcc agaaggcgcc gtccatgccg gcgttgactg gaatccacca atcggcgtgt
    14281 ttggaaacct gactgaagtc cggcgacaga accaccagtt tggcgccgtg gtgccgcgct
    14341 tcgacggcaa aatgcgcgtc gggagtacgg gtcatgttga ggttactgcc agtgacgacg
    14401 atatatttgc tgttgaacca gtccgcgctt tccgcaacgt cggtcttctc accccaggtc
    14461 tccggagagg cgggcggaaa atcggcatag agatcgtaga agctgagcat gacgccgccg
    14521 aataactgca agaagcggga tccggcggcg tagctgatca tagacattgc cggaatgggc
    14581 gagaagccaa cgacacgatc gggaccgtat ttcttggccg tatacagcat ggaagcgaca
    14641 acgatctcca ggcactggtc ccagctcgca cgccggaatc cgcctttgcc acgtgccatc
    14701 tggtatgcgc cgcggctttc ttcgttctcg acaatcgatg cccaggcttc aaccgggtcc
    14761 ttatgcctgg ccctggcggt ctcccataga tccatcagca ctccgcggag atagggatac
    14821 ttgattcgca gaggactgta tatataccag gagtaaacga tcccgcgttg acatccgcgc
    14881 ggctcgtaca gaggcagacc gctttccagt ttgggataat caatcgcctg catctcccag
    14941 gtgacgatgc cgttcttgac aaagaccatc cacgaacagc cgcccgtgca gttgactccg
    15001 tgcgtgctgc gtacgacttt gtcatgctgc caccggttcc gatagaactc ttcccaagcg
    15061 cgcagctccg gattcacgat atccttgatc cagctcaaaa tcgaactcct cgctccatcg
    15121 ttttgtggac catagtccgc cgcaccgatc gcacccgatc tcgccagact atccctgtga
    15181 gaaccacaaa aactgtggct agaccgagac cgatgagcag caggatctga gtattccacc
    15241 gcggagccgg attggatcca gcgtctgcaa gaaaggccac cagatccgtt tgctcatccc
    15301 gggtgagcgt gtgcgcacgg taaataggcg tcatgacgcg gaagtacaga gtctgcatcg
    15361 ccgcgtgcgt accttcagcg ccgagtttgc gatacgcgtc cgtaaggtcg ggaccgagcg
    15421 tgcctccatt cgggaacggg agtccggcga tggtatggca gccgatacat gcggggcctt
    15481 gattgcgaag aggaagcgtt ccggtgaata gctgggcgcc acgctgaatt gcacttcggg
    15541 cggaatcgga tgccggcggg gctggctgag cccaagcagt cgcggggagc aaggaaataa
    15601 cagcggcggc caacattcga cggccggaat taccggtcca gcggccaccg gtataaaagg
    15661 tgggctcggg atggcgagcg ctggttttcc aatgcaccga ctcgggtact ccgttcacgg
    15721 aaatacagac gcccccggga tgcgggaggt gcgccgatcc tgacgccgat tatgttccga
    15781 agacagcgca aatacaatga atcagttgtc actcgatgca ggacattagt aacggagagt
    15841 cgcgaatcct cttgaggctg cgaacaaacc gctccgtgcg gttgagatat tgcgcaaagc
    15901 ggagggcagc cgccgatccg gatcaaaaat aaaatttcgc gccgagttgg atgattcgcg
    15961 tcgcgacgcc ctgcacgccg ccgccgacgg ctccgaacgt tctggagcga acgccgcgat
    16021 cgaccgagcc gggttccggg ttgaaatggc gaaacacgtt gaagaactcc gacctgagtt
    16081 ccaaacggct acgttcaccg aggcggaacg acttgtcgat tgaaaaatcg gcgttgatca
    16141 gtcccgggag cgtgatcgcc cccgtgcgga cagaggttcc gaacctgccc cattcggcca
    16201 gcgtaaaggc gtcagcgttg aaccagcgtt cccaggtgcg ctgatcccgg gacaggttgg
    16261 gcgactgacc aacaacaatg tcggcgcgag atccctgacc ggttccggtt gtgtcgcggc
    16321 cgtcaacgat accagcggga aatccgctct gtgccgtgat gatcgttgcg aacttccagc
    16381 cgcccgtaag atgacgggca aaggctccgc cgcccaaagc cggcaattcg tagagcaccg
    16441 tctgtacaaa ccggtgacgc agatcgaaac ccgacaggga atgttcgttc ttgagattgt
    16501 aatagtcctg tggcgttccg atgaaggacc cgttcccgat gagcgccccc tgatcgtgag
    16561 ggccggacaa cgactttgcc caggtatagg cggtcaggaa agtgaatcct gacgacatgc
    16621 gccgttccag tttggcttgc agggaatggt agtttgaatt gccgatcgat ttcaccgcgg
    16681 aaaccgcccg ggggaacgcc tgattgggcc tgcgcgcatc gatgccggcg agccccggcg
    16741 ttcgcgggtc gaccagttcg atgggccggt tcaatgcgag agcaccaggg tcaaaggcga
    16801 tcgagagccg tgttcctttc gaaccaacgt agccaacatc ggcgagcacg ttcccgggaa
    16861 gtctcctttg aaccgtgaaa ttccattgtt ggatgtaagc atcgcgcatc aatgggtcaa
    16921 tactcgtggc gcttgcaagg tttgccgagg ccccctgagc ggaagcaaac ggatcactcc
    16981 agagaacgtt gggcgcgcct gacggattgc ccaggatggt cgcaccggca gttgcctgag
    17041 cgccctccac catcgttgca ttggcgttcg ggtgttcctg ctgataatag attccgtatc
    17101 cgccgcggac gactgtttct ctgagccagc ccggcctcca ggcgaagcct atccggggac
    17161 cgaggttgtt cctgtccggc gagagaaggc tgcggccgaa cggggatgtt gccggagtga
    17221 tgaacccggt gacggcgaat ccattctgtc cgatgttgac gattctgtcg tcgaactgcc
    17281 gccatctttg aaagtagtcg tatcgcagcc cgagcgtcag cgtcagattg gacgtcacct
    17341 tccattcatc gttcacataa taggactgcc acaagctgtc catgtcaatg acagtgggga
    17401 ctggattaat ggtggcgctg atgagatatc cgaggagaaa gtcggcgagt ccgctgccgg
    17461 tgaacgatcc gtcaaagcga tagaggcccc gtggatcgcg agcttgctcc tttgtgtaat
    17521 agcgcttgtt aaactcggcc ccgaacttca gaaggtgccg gccgcgctgc caggagacgc
    17581 catcggtgat ctgccagacc tcgtaaccgc ggtcgcgcgg tccaatctgg ttctgaatat
    17641 caaaaacact gaacccgcct tccgggccgt cgatctgaac gcggggcgga ccgaaatcct
    17701 ccggccgccg cgaggtgagc gggattccca gttgaccggc tatatcgaac gcgggattat
    17761 tcgttgttcc gaatagctcg aactcctgca tgcggttcca gccgacccgc aactggttga
    17821 cgaagagagc ggtctgcgcg cgcacctacg aagttgaaac gttctgcgtg cgggccaaat
    17881 tgttgcgctc gtcattaccc caaaacggcg tcccgctttc cacagtgtcg ttgaacacat
    17941 agcgggcgct cacaatgtcc ttggtggaaa agttgtgatc gacgcgcgcc gtatagttgt
    18001 cctgcttgga taccgggctt tgcggcgtgt tgatgaaatt gaagattccg ttctgcgtat
    18061 tcggcattgc cgtataggac aggatcgtct gcgcctgtgg gctcaacgcg ctttgcggga
    18121 ttctgttgtt gacaatcccg acgcctaaag ggtcctttaa agtcaaaggc tggccggttc
    18181 gggcatctac aaggccactc aggtcgccgt tgcgctgcgc ctgggtgggg acgatccgga
    18241 aacccgccac cgctcccaac gcctggcgcc ccgcctccca gttgaagaag aaaaaggtcc
    18301 gatcggtgcc gttgtagagt ttgggaagcc gcacgggacc gccgatgttt gcgccaaatt
    18361 gattgcgatt caaccgcggc gccttcagat ccactctgcc gatggcgttg tacgcggacg
    18421 tcagggcgtc atttcgattg aactcccaga gagttccgtg aaaagtattg gtaccgcttt
    18481 tggtgatcag gctgatctgg gctcccgggg cagctccgtt ttcggcggag tagctgcttg
    18541 tttcaacctt gaactcagca agagaatcga tggagggcga gaaagcatag gaaaacgcat
    18601 cgccatccat cgactcgact ccgtcgatga aatagcgatt cgccgtgtcg cgaattccgt
    18661 tagcagacat acccgtcgat ccaaagggcg agtcgacctg tccaatggag ccacgtccac
    18721 ggcgtaccgt gatcgagccc ggcgtgccgg gttgaatgcc cggaataaga tgcgccagtt
    18781 gcgcgaagtt tcgaccgttc agcggcaggt cgacgatttt ctgtccgtcg aggacggcgc
    18841 ccacagtcgg gttttccgtc tgcaggactg ccaccaggcc ttgcacctct accgtctgag
    18901 aggtgtcgcc tacttgcaac tgaacgtcca cgcgactggt ctgccccacc tgtagtgaaa
    18961 cgccatccac gacgttctgt tggaagcccg aggccataga ggtaagacga taggttcccg
    19021 cgggcaggaa ggggacggag tagttaccgc ttgcgtcggt ccgggtctgc cgcgatgcgt
    19081 ttgtattgac gttaacaatg gtcaggacgg cgttcggcac gacagcgccc gatggatcgg
    19141 tgacggctcc aacgatggat ccggtgatct gctgcgcggc ggcggagacg tgcaacagaa
    19201 tagacaggac ggtaatagtc agcgtctggc gcatggcaaa ggacctcact tgtctacggg
    19261 cagacgcggg ttggatggaa cgaacctgta accgcgggca gcccgagcta gcagcaagta
    19321 tttggcctga gcagccgcca agcaagttcg aagtatgtat aataccgtta acaactgggg
    19381 caaatgccac ctttctcatt tactacctcg catcggagag ggccgcccga acctgtccac
    19441 agtcccacat tcgaagaaca acaacagcag gttgggcgag tgtgccagag cacggccttt
    19501 gccggttccg aagtgctgcg taacatgctg tcctaccttt ccagagcatc cgccgagcac
    19561 ccgggacgac ccgctaaaga atacgaactc ggagtggcgg tgctgggcag aaaagacgga
    19621 ttcgatcccc ggctcgattc gagtgtacgc gtccactgcg cacggctgcg gacccggttg
    19681 ttggagtact acctggagga gggcgcctcc gatccggtgg agctcaccat tccgaaaggc
    19741 gcctacaacc ttgttgcgcg ctatcgagat gttccgcgta agcctgagat ccagacgacg
    19801 gttttgccga ccatgccggc gcccaccgga cggcacccgc tgctttggac atcctgggcc
    19861 gtctcggcgg ttgcggtcgc cacggcgatc tggttttggt cgcttctccc ccctccggta
    19921 agcgccgagc tgaagcattt ttggaataca ttcgactcgc gcccggacga gacactcgtc
    19981 gtgttctcta atccccgctt tgtcggaagc gtgtcgaacg gcggactgct ctatgagcct
    20041 cctaactccc aggttccttt gaaagagatc aacgatcgct atacaggaat cggcgaagcg
    20101 atggcgctac agcaactcac cagacttttt gaaaggcttc ggttaccgct ccgggcaaag
    20161 cgaagcggtc tgcttacgtg ggatgaggcg cgaagccgca actttatctt catcggtgcg
    20221 cccgaagtaa accggcctca aatggaactg ccgaaactcg agcagttcgg gttcaaaccg
    20281 gccgatgaag agccgcagtc agggcgtggc ggaattatca atcttcagcc acaggcgggc
    20341 gaacagcaat actatctaaa ctccggtcct ccatacacgc acgactatgc ggtcatagcg
    20401 ctcgtgcccg ggctgagcgc ggggcgccgc gctcttatcc ttgcgggccg aaccacgtac
    20461 ggtacacaag gagccgtgga gtttctgctc gaccaaaaga gcacatccgg cctgctcagc
    20521 aaactggatg cgcaacggtc gaggcttccc ttcttcgagg cgctgttgcg cgtcaaggtg
    20581 agcggcggcg tcccggtcca gccggagctt gtcattctcc gcaggcggta attgatccgc
    20641 tcccggcacg gccctttgat acaataaatg ctccctcatg agtgacgtta cctccagagt
    20701 tccggtctat gcctactgcg agtaagaaac ctttgaagat tgccgtttgc ggcctcgggt
    20761 tcatgggcag tacccatctg aaagccctga agaacgtgaa agaggccgag cttgttgccg
    20821 tcgtcagcga cgtgccggag aagttaacgg gtgacctcag cgcgattcag ggaaatctcg
    20881 gcggcccggg agagaagtgg gacttttcgc atctcaagaa atataacaac gtgtacgacg
    20941 ccatccggga caaggatatc gacgcggtcg atctctgcct gccgacccat ctgcacaaac
    21001 cggtgacaat cgaagcgctg cgcgcgggga agcatgtact ggtggaaaag ccgatggcgc
    21061 tcgatggagc cgaatgcgac gagatggtgg aggagggtga ggagtccggc cgcgtgctga
    21121 tggtggcgca ggtgctccgg ttctttcccg agtatgtttc ccttcgggag actttgcggt
    21181 ccaagacgct tggagaggtg cgggcggcta ttttccggcg ccggtgcgcg ggtccgacat
    21241 ggcagcggtg ggtgtacgac aaatcgcgaa gcggcggcgg cgttttcgac ctggtgattc
    21301 acgacgtgga cttctgcctt caggtgttcg gacaaccgga ggccatttcg gcggtcggct
    21361 acgagaagct ggagaacggc gtggactggc tgacgggaac gctttactac cccaacatcc
    21421 cgggcggaat cgtgattcag ggcggctggc accacccgaa gacgtttccg ttttcgatgg
    21481 aatattcggt ggttgccgac ggcgccacgc tggaatacag ctccgccgga cgtccggcta
    21541 cgcagtacaa cgctgacggc gagaagccga tagacgtgaa acagcaggac ggatacgaag
    21601 cggaactgcg atactttgcc gagtgctgcg tggagggcaa gaggcccgag ctatgcccgc
    21661 cttcggaatc gtctctggca gtgaagctgt gccaggcgct ggtggacgca cgcggcgaaa
    21721 acggcaagcg gctggaatgg aaaggctgaa gcgggcgtaa gggcaaccgc tccgtgaccg
    21781 tcgcggctca gcgttttttc cctgcgatct gccattcggt aaacggcgaa cgcgtgccct
    21841 ggacattcac attcagcaga taaggacggc gtgacgcgaa agcgcgctcg accgcgggcc
    21901 ggatgtcctc aatccgctcg atgttctcgc cttcgccgcc gaagcctttc atcaccaggt
    21961 catagcgcgt gcgccgcaat tcgcatccga ccgtctcgcg atcgggatag aattcactct
    22021 gcaactcccg ttcgagtccc cagcccgcgt cgttgccaac aacgatgacg atgggcaggt
    22081 tgtaacggac gatggtgtcc aactcggcga tgtagaaacc gagcgaaccg tcgccggtga
    22141 tcatgacaac gggccgggcc gggttcgcca tctggagcgc aatggagttc ggtaacgcgg
    22201 agccgatggt tccgaggggc ccaaggcgca gccagccgcc gggcgtcaca gcgggcagga
    22261 tcgcgcgacc ccagtgggcg aagtctcctc catcccagga aaatagcgtt ccttccggaa
    22321 gggcgcgctc aaactcccgg taaaatgccg ctgggtgcat gggggaggaa tcgccgccgg
    22381 cggcgttccg aagatcgtcg cgccaggccg ataccaactt acgctgccgt tcgatccact
    22441 cctctcgcgg ctcccatccg cgttcgccga ggcggtcgca catagcttgg agagtctgct
    22501 tgagatcggc gcagatggcg gcttccaggg tccggttgag tccaagctca gcggagtgaa
    22561 tgtccacctg aatgaatttg gcttcgggac ggaacaggcg ggggccgccc aatgccagcc
    22621 ggtagtcaat acgcttgcca agcacgagaa ccacatccgc ttcctgaaac gcgacatgaa
    22681 ccgccttgtt cagggcgggg tccgcgtagc ccatcgaata gggctcaagg tcggagacga
    22741 tgccgcgcgc catcgtgatg gtgtagaagg ggagccggga atgcttcaga aattccgaga
    22801 actcctgttc ggcgcccgcc caccagacgc cgctgccggc gatgacgatg gggcgcgtgg
    22861 cgtttgcgat caactcgatc gcgcgatcga tttccgaatc cgcgggtacg gctgcgggcg
    22921 cggcgtaggg ctccggcatc cggagaggct gcgcagcctc ggcgacgaac aggtccaccg
    22981 ggatggtgag atgggcggtg cccatgcgcc cggccatcga ttcggcatac gcgcggccga
    23041 gatggaacgg gatctgtccg gcggaggcgg gttgcgccgc atacttcgcc acgggcgcgg
    23101 ccatgcccac ctgatcgatc acctggaaca actgccgccc ggccatggag gccgccggcg
    23161 cgccactgac cagaatcagc ggactcgcgg caagatgggc cgttgccagc cccgtcatcg
    23221 cattcgtatg tcccgggccg cccgtgacga tggcaagagc gggttttcga tgaatgcgcg
    23281 cccacgcatc ggcggcgtgc gcggcaccgg attcgtgacg gaagtcgatc acccgcacgc
    23341 cgcggtcggc ggccacacgc aggacttcgt tgaggtggtc tcccaccaat gtaaaaaact
    23401 cgttgattcc cagacgctgt gcagcgtcga cgaacaattc ggcaccagtt ggcatatcag
    23461 atcatttgat cgtatgtgcg ccggaggaat ccatcggtca tacccgcaat ataatcgcag
    23521 acaacgcggt gcacggaatc ctcggtggcc tgctcgacat aaggctcggg cagcttttcg
    23581 ggatctttca tgaacgcttc gaacagggcg gtaatcatac cggccgatgc ctgcctttcc
    23641 cgcaaaacga catcggactg atacaccttg gtttgcagga agctcttcag agctgtattc
    23701 gtggcctcca cggccggagt caggcgcgca atgcgggaag agtgatgccg gacatcgtcg
    23761 acggtctcta agccggcctg ccgcgcggat tggctggtgc cttcgatgag cccggaggcg
    23821 agcgtgtcga tcagcccgcg gaccacttcg gagaagcgga cccgctccgg cgcgcccggg
    23881 aactggaact ccatgttgtt ccagacctcg gcgaattcgg gaacggccgc gcacacatcg
    23941 tcgaccgaga agagccgggc ggtgtatccg tcgtcaaggt ctgccgtgtt gtaggcgatc
    24001 tcgtcggcgg gatcgatcaa ctgcgcctcc acgggaggct tccggccggg caggtattca
    24061 tccagcatgg gaaactggcc ggccttgaaa tcgcgggagt gctttgccat gccttcccga
    24121 acctcgaagg tgaggttcaa ccccgggaag cgggcgtagc gctgttcgaa ggtctcgacg
    24181 attcgcaggg cgtgcagatt gtgatcgaaa cggtcaccgt acttccgcat ctggcgattc
    24241 agctcctgct ctcccgcgtg gccgaacggc ggatgcccca ggtcgtgcgc cagggccagg
    24301 gcctccgtaa aatcctcgtc cagatcgaga accgcggcgg cggtgcgggc aatctgcgag
    24361 acttcgatgg tgtgcgtcag ccggttccgg aagtggtcgg agatgccggg agggaagacc
    24421 tgggtcttgt cttccaggcg gcgaaatgcg cgggcatgga caatgcggtc gcggtcccgc
    24481 tgaaactcgt tgcgatacgg atggagaagt tcggggtagc ggcggccccg agcctggctg
    24541 accggaaatc ggagatggcc gagtgtcggt ttcatctgcc ggggcggctt gctactgagc
    24601 tggaatatcg gagagagccg tcagtgccat gcggctgaca gcactgcgct ccgtgcgcag
    24661 cggttcgagc aacttccgcg cttccgcggg attcttcggc gcgattacct ttgcaagagc
    24721 aatggtggcc tgttctttcg ataccagcat gctgggacgg tctatcagcg cacgaagcag
    24781 tttttccgcc tcggcggtct tgccctgccc ctgataaagc tgcgccagcg cgaactgcgc
    24841 aagcgatgag taaggattgc ggcccgcatc ggccacttcc tggaaatgct tggcagcgtc
    24901 cgcttccttg cccgcttcgg cagcgatcac acccaggtaa tagcgcgcga tagtcgcctc
    24961 ttcagagcct gagtggtcat tgatgatctg ctggaacgcc tttgtggctt cctcgcggcg
    25021 tgcttcggcg gtagggaatg ttttcaccaa tggattgccg cttggaggcc ccacggcgga
    25081 gttttgaata tccaatgcct gggtcaaagc ctgctggcgc acaattttct gtttaccgga
    25141 ataccagtac gcaccccaac cgacgagcaa tacggcgagt gcgataccgc cgatgaggat
    25201 gacctggcgg cggtgctcgt ccagatattc gacagtactg ccgacacgct cctgaaaccg
    25261 gtcgctcttc aattctttac ggctgattct atccacaaga aacctctggg gaacttctat
    25321 cttatcattc gctcgtggcg gcgccggcgc aaaggacggg agcagctcag cgggttctgg
    25381 cccctgtcgc ggccggcctg gggccgtttt ccggtgcggc gggcgccggt gtttccggcc
    25441 ggatcaaacc cagaaccttt ctcaattccg catccacctg gtcgcgggta tccgcgtgat
    25501 ccttcaaaaa ctgccgtgcg ttctccctgc cctgcccgat ccgctcaccc ttgtagctgt
    25561 accaggagcc gctcttctcg acgacgttct gagcgacgcc gaggtcaatc agatcgccct
    25621 cgcgcgacac tccctcgccg taaagaatgt cgaattcggc ttcgcggaac ggcggcgcga
    25681 gcttgttctt gacaatcttc atcttcgtcc ggttgcccgt aacggtgtcc ccgtctttga
    25741 tggcggcgat gcggcgaata tcggcgcgca cggaggagta gaacttcaac gcgcggccgc
    25801 cggtggtggt ttccgggttt ccgaacatga cgccgatctt ttcccgaatc tggttaatga
    25861 agatcagcgc ggtattcgac ttcgagacaa cgccggtgag cttgcgcatg gcctgcgaca
    25921 tcagccgcgc ctgtacgccc atgaaggaat cgcccatttc gccgtcgagt tcggctttgg
    25981 ggacgagcgc agccacggaa tcgagcacca gaacatcgat ggaactggag gcgatcaggg
    26041 cgttggtaat ctcgagcgcc tgctccgcgt agtccggctg tgaaaccaaa aggttgtcga
    26101 catccacacc caacctggag gcatagccgg ggtcgagcgc gtgttccacg tcgatgaagg
    26161 cggccacccc gccattcttt tgcgcctgcg cgatcacctg cagcgcgatg gtcgttttgc
    26221 cggaggattc cgggccgaaa atctcgctga tgcggcctcg cgggaagccg cctacaccga
    26281 gcgcggcatc gagagaaatg gagccggtcg aaatggccga tacgggaacg atggcttcgc
    26341 gcgacccgag ccgaagaatc gagccctttc cgaactgctt ctcgatttgg cccagagtca
    26401 caccgaggac gcgggcgcgt tccttctcat ccattacgtg ttttccttag cggggaatcg
    26461 gaaaatccga ttataccagc ggctcccagg gaagggtgaa agccgcttgc ttgctcgcgc
    26521 gcggctccgt tccgattaga gctttggcgc gtagtaacct tttctcgcac ggaccagcag
    26581 gcccttacga tctttcacct tgacttccac ctcatgatat tggccgtcgg cgcgcagagg
    26641 ctccggccgg tagagcaggt tgtactggtg gcgaagttcg ttggcgatgt tttcaaacga
    26701 ttgcgagagg tcctgcacct tgaacggaaa aaacgtgaga ccgcccgttt cctcggcgaa
    26761 gtacttcaac actttgtcgc cctggctcgg tatgcgggtg atgttcgtgg agatcgtata
    26821 gatcaccacg tccgctttct gcgccatttc caaagcctga tcgcgtgtgt agcggctctg
    26881 gttgtcgtct ccgtcgctga gcaccaccat ggcacggcgg aacttgtgcc gcggctggtc
    26941 gagcatcagt ttatcccggc aggcgaaaaa gatcgcatcg tagagggccg tcccgccacc
    27001 gggccgcagg tcgcggatgg cgttttcgag ctgccgctgg tcgtccgtca ggtcggctac
    27061 cagttccgca gcggtatcga agctgacgac gaccgctttg tcctgtccgg ggcgaagcag
    27121 actctgaatg aattccaccg ccgcttcctg ttcgaagcgg aagcggtcgc gaatgctgtt
    27181 gctggtatcg atgagaatcg ccagacgcaa cggcaggtcc gattcggcgg tgaactccag
    27241 gatttcctgg cggttctttc cttcgaaaac caggaagtcg tccttgatga ggtccgttac
    27301 aaaccgccct tttcgatcgg ccaccgtgaa caggatgttc acacgcgtga cgtccaggat
    27361 gatgcggttc ggatcgtcgg gcgccggcgt tccctttttc tggtccacct cacctgcgac
    27421 acgcaagctt ccggcgagca gagcaaagaa acagactgcg gccgtcagca gccgtctacg
    27481 gaattgcatg aaaacctcta ccgacgatta tatcggaccg gacctgtttt tgagccgctc
    27541 gatccaaagc ttcaactcct ccggaagcgg cgcctccagc gtaatttcag ccccggttga
    27601 cgggctgtgg aacgagatgc gctgcgcgtg caggaagaag cgttctgttg ggggttcgct
    27661 gcggggcgcg ccatagagac ggtctccggc gacgggatgt ccgatggcgg ccatatgaac
    27721 gcgaatctga tgagtgcgtc cggtggcgat gcgcacttcc agcaaggtga aatcttcgaa
    27781 ccgctccagg acgcgatact cggtgagcga ttcgcgcccg cggccggact tcacgctcat
    27841 gcggattcgc cggacagggt cgcgggaaat gggtttgtcc accttacccg cgtcagcccg
    27901 cacgtttccg tgcaccaggg cgaggtaggt cttgcggact tcgcgggatg caaactgatg
    27961 ggccaggcgg cggtgtgcga aatccgtacg ggccaccaga agaacgccgc tcgtctgccg
    28021 gtcgagccgg tggacgatgc cgggtcgcag gtccccggcg acggctgaga ggttctggaa
    28081 ccggtgcagc aacgcgttca ccagagtgcc cgagtggcgt cctgcgcccg catgcaccac
    28141 gactcccgcg ggcttgttta cggcgacaag atcctgatct tcgtagagga tgtcgagcgg
    28201 caggtcttcc ggctcggcgt gcaaaggagg aagcgggccg ggctccacgt caatcgtctc
    28261 gccgccgcgc agcgtgtaag aggacttgcc gggccgtccg tccacgagca ccagaccctg
    28321 gcggatccac tgctgtattc gcgccctgct gtattggggc aggtggtctt tcagaaagtg
    28381 atcgaggcgc ttaccgcggc cggacgtggt ggattcgagg cgcacgttca aatcaacgga
    28441 gaaatcaagt gtttataatt cagatgtgat ccgaagcgca attgtacctg ttgcgggcta
    28501 tgggacgcgg ctgctgcctg ccaccaaatc gcaaccgaag gaaatgctgc cggttgcccg
    28561 caaaccgatc gtgcagtatg tggccgaaga actggcggca aacggggtcc ggcagattct
    28621 gttcatcacg gggcgcaaca agagctcgat cgaaaaccat ttcgacagcg acccggagct
    28681 gctgcgtatt ctgaaggccg ccaacaagcg cgacctgtta caggaactcg gattcgagga
    28741 tctcgacgtc aaataccttt atacgcggca acggcagcag atgggtctgg gcgacgcggt
    28801 gctgtgcggt gaaaattttg caggggaaga gcccttcgtg gtggcattag gcgactccat
    28861 tttgggcttg catgcgcaat cgcgctcggt gtcgcggatg gcggaccttt tcgaatcgcg
    28921 ccgcgccagt tgcgtgattg ccgtggaaga ggtgcccatc gaggagacgc ggcattacgg
    28981 aatcgtgcag gcgggcgacg gcgaggggga agccggcgcg taccgcgtgc tgaacctggt
    29041 ggagaaaccg cggcccgagg aagcgccgag caacctggcc atcgcagggc gctacatctt
    29101 ctctccgttg atcttcgaca tgatccggcg gatcgcaccg gatgcgcgcg gcgaaattca
    29161 gttgacggac gcgattcgga tgatgtgcga ggaaggaaag cgggtgctgg cggtgaaact
    29221 tccgccggga gagaagcgct acgacatcgg aaacttcccc agctactttg aaaccttcgt
    29281 cgaatttgcc ctggcggacc cggtgtacgg ggccggcttt cgcgcgtcgc tcgaacggct
    29341 cctggaagac aaagcgcgac aggcactcaa gtgaccaccc gcgcggtctt cgcgctcatc
    29401 gccgtggtgc tggttgtctc acggctatgt cattcgcaag tgctgtgggc ggaggaaggc
    29461 ctgccgctgg cggcggccct ccagatgata tccggcaagt ttctgtacag cgaaatctgg
    29521 ttcgacaagc ccccgctgac aactgccatc tacgtcctgt ggggagcgca cacgggatgg
    29581 ctgctccgac tggcgggcgc gctctacgcg cttctggctt gcgtgctggc gtatctcatc
    29641 gcacgcgatc tgtggagcgc ccgtgaaggc agaatcgccg ccgcgctcct ggccttcttt
    29701 ttgactttcg gcatacaatc cgcggtcatt ccgctggcgg cggacctgct gatgctggtg
    29761 ccgcatcttg cggcggtatg gttcgcctgg cagcggcgcg cgctctggag cgggctcgcg
    29821 gccggcgtgg cgcttctgac aaatgcgaag gccgccttcg ttattctgtt ctgcggcgtc
    29881 ctgctttacc gctacctggt gcgattcagc ctgggggttt ttataccgaa tgccgccgtc
    29941 cttctgtggc ttcagcaggc tggcgcattg gacgattaca tcctgcaagt atgggaactg
    30001 gggcggctct attcagccca tacgttcgtg acggaaccgt ggaaggaagg cgccatccga
    30061 acgctgaact gggcggggtt ccatgccgct ctcatcctgc ccgccgcctg gttctggagg
    30121 aaggaagccg gatcgcagcg cgcgcttttc gccctgtggg cggctctgtc gctggctggc
    30181 gtgttcacgg gctggcggtt cttcccgcgc tactattttc agcttctacc tgtattcacc
    30241 ctggccgcgg cacgcggtac cgccgggatg ggacggatgc gctggcttgc cgttctggcg
    30301 ctggcgattc ctttcttccg tttcgcgccc agctacgttt cgcttggtgc atcggcgctg
    30361 gccgggcagc ctagcgggtg gcgcgacgtg gcgctggacc gggacagcca ggacgcctcg
    30421 cggattctta tgcgagagac cgcctccggc gatacgttat tcgtgtgggg tttccggccc
    30481 gcgatctatg cctacacgag aatgccggcc gcttcccgct ttctcgaatc ccagcccctg
    30541 accggcgttc tggccgaccg ccacctgagg cggacggatg cgttgagcgc gggctggatt
    30601 cagacgaaca gaaaggaact gacagaaagc cggccggcct ttatcgtcga cggtctgggg
    30661 ctctacaatc ccggcctggg tatcacacgg tttcgagatc tggatctatg gttgtccaac
    30721 tatgaaattg caggcaagac aagggggacc atcatttacc ggaggaagcc ggcttccaaa
    30781 taggcggggt cctccgtccc cgcctattcc agcaacgagc acctgaagcg caataattac
    30841 tgctgctgag caggcggctt cgtgcgggat tccatattta cgccttcccc ttttagatca
    30901 agggcgtaga cctttacatc tactcgcctg ttctcctgac gttcggcccg cgtcttaccc
    30961 ggggtgggcg cttcaccaac accaagcacg gagatccgcc gtaacgggac gttatgctcc
    31021 gttgccagat accggactac cgcggaggca cgtttctggc tcaactcgag gttatagttt
    31081 gatgagccgg tgtggtccgt gtagccctgt atttctacaa caaaattctg catgttgcca
    31141 aagctgcccg cgatagaatc caagtcggcc ttcgcctcct tggtgaggac gctccggtcg
    31201 aacccgaaat aaaccttatc ctcggtaacc agccggtaat tatccaggtt tccgaaggtt
    31261 ctatcgacgt tctgttgtac ctctccgagc cgggtctggg tctgctccgc cagtccatag
    31321 gcgccatccg cgcgctgacg ggcctgaccg gccgcgtcgc ctgcctgtgc tgccgcctgt
    31381 ccggccgcgg acgctttctt ctccgcatcc atcgcgcgct catccgcgcg ggacacctcg
    31441 tttcccaact cgcccaacct cgcttgttga tcctggttct gcttctcaac ttcgccgaca
    31501 cgctgttcta caggcgccac cactttcgcg acatgctttt ttgtggcgca acccgtcccc
    31561 gtcactccca aaagaaggag agcccccagg gaaacaccaa agagctttgg actagccata
    31621 tgtttctcct cgtcgaacct cgaatgcact tcaactgcca gcccacatgc aagctgtttt
    31681 gaggtactta gacccactac aacgccgctg ttgggcaaga attacccata gtacgtgtaa
    31741 ctttggtact tgggtcatga gagtactaga tcatagtagg ggaggcgagg ggcggcgtca
    31801 atggcaagct gcttcaaaac gctggattga gctggtttga cctgaggtgc cggtccgcgg
    31861 tatcttgaac agagaacgtc aaacgtttgg tatttcctga atggccaatc atatttctgc
    31921 cctgaagcga atgcgcgtgg agcgccggag aacgaagatc aatcgggctc gtaagacccg
    31981 tttgcgccac caggttcgcg caatgcggcg cctcctgaac gataagaacg gagagggagc
    32041 gcaagctgcg ctgcccgcca cttattcgat tgtcgaccgc gccgcgaaat ggggcgtcat
    32101 caaaaagaac acggctgcgc gttacaaggc gcgtctgacc gggcggttga aagctgtatt
    32161 ggccgccagc gctcagtagc tcagccggca agctccagaa cgaatctctc cattacgatc
    32221 cggtcgtccg ggttggcatc tctcaacgcc ttatccgtct catagatgcg acggatcagc
    32281 gtctcgagtc tctctttcga aaaagcagca gccgtctgtg ccacctgttc ggcacgcgac
    32341 cgccacatct gcaccccctg acgggagaag tgcccctgaa tctgcgacgc cgaattgaga
    32401 cgcacttctt tggccaccag cgccaggcgg aactgggttg cgaggaaggt caacaccagc
    32461 ggaagatact ccccttcgcg aaccaggaaa tcgagcagag tcaacgattg ctcgcggtct
    32521 ccccggccga gtgatgctac cagggcaaag atgttggctg cccgggcatc cggaaccatc
    32581 agaaaaatat cttcgcggtt caccggcctc gtgccgcccg tatgaaggcg gagtttctcg
    32641 atctcgacgg caattctcgc tacgtcggcg tccaatgttt ccaccaacag cgcaagttca
    32701 ggggaggcga ttttaagatc ggcctgccgc gccagagatt cggcggtctg ccgtgccgag
    32761 tccagggaga aacgttcgaa ctcaacatga tttgggactg cggcgtagaa tttgcggacg
    32821 cggtcaagca tcgacttctc ctcactcccg gatccggacc gcgcggcatc aaagacgaga
    32881 acggtatcgc cggaaggcga ctgcatgtag gtttcgagaa gcgccttggg accggttttc
    32941 gaaggtgagc gttcggagcc gtcctcctcc gccgacgcgc gggtgcgcgg gagagcgact
    33001 tcagcgctcg ccacccagat cacgcggcgg gtggcaaaca gagacaagga tcgggcgtcg
    33061 tcgaggacct cggccaggga tacctcatcg agatcgtggc ggacaaagcc attctcttcg
    33121 ccgggcccga gcatggcggc cagcagcgcc tgccggcagg ccgtccgctg gtaatgctcc
    33181 ggcccgacga ataagtaggc gggagcggga tgggagcgct gaacatgaga aatgaactgc
    33241 tggggagtca tacgctaaaa gcttgtcaga atggcgctca caattccgcg tgcgacatcg
    33301 cggctcaggc gctcgccagc ggtatcgctt tcttcaaaat acgagagttc cgacacactg
    33361 atttcgtaac gctcgcgaac gaccagaccc ggccgcgcaa acaggatttt gccggtggta
    33421 cgttcccgca gcgatatatg aagtgtgacg ataatctgga ctccgctggc gcggcccgtt
    33481 ataggatcga acacttcggg atacgacgca tagttcgtga cggcgccgtc catgatcacg
    33541 tcggcggtat ccgggtccga agacacgtta tatttcgttc gtgtgatgaa ctcacgggtg
    33601 agcgccgaag cgatgcgatc cgccagtttg ggtctcgagg tcaggttctg aaaggccgga
    33661 atcgcgatca cgcgagtggt tggcggcagg acctccgacc ggccggtgat acggtagccg
    33721 caaccggccg aagcaagaag gacggccgcc agagcagccg cattcttcac cgggagcctc
    33781 cgccgcgagc cagcaggtcc ctggcaacca tgacggcgtt ttcggcggag atccgcaggt
    33841 tgtcggcaac gacccagaac cagcaggcgc ggggattgtt ccggtccgct tctatcgcgc
    33901 ccagagtgat tccactctgg ccggccgcgc cgacgttcga aggtggctcg aggccgtcgt
    33961 cgcgaacctc tacgcgcggg gacgcgaggg cgtcgcgaaa acgtgcaatg gaggggggcg
    34021 tggcaaactc tacccatgcc gagatgctat agccgtggaa cacgggcgcc tgtaccaacc
    34081 ggagcgatgg caaaggcgca tgtgaggcga tgccgaggag tgtcgccagg tgccggtcga
    34141 tacggacttc tacatcctgg agcttctcgg cagcctccgt tccatagcgc ggcagcatgt
    34201 tgaacccaag ctgcgcgtcg aatacctcct tcggcatcgg cttgaacgag aagaggttca
    34261 cggtctgctg atgaagctca tcgagaccgc ggcgtccgcg ctcgctggcg ggctcaaaga
    34321 tctgaatgac agaccgcgcg acgggctcaa taccgtgcag gcgatcgaga aacaaagcga
    34381 tggtgatggc ggccggatgc gcaatcacgt ggatggccgt tgcggccaca tcgtacccac
    34441 cgggctcgat catgggcgcc cgcagtctgg cttccgggcg gtcttcgagc gcgaagctca
    34501 agtccaccag cacgggcgtg cgtccggtat ccgaaagcag atccagaact ttccaggtgg
    34561 ccgtgccggg gccggccatg aacgccagtc cggcatccat caggtgctcg cgctccagag
    34621 gtgcgatgaa cgccgcctcc ttcgcatcct gtgtgaggat gccgcccgtt tcttcggctc
    34681 cgatcagcgt gaccgtctgg ttggcccact tatccgaaag cacctcgcgt aattcccttc
    34741 cgagaagcga ttcgctgccg acgatggcga cggatttaga catcagcgga ctccattccg
    34801 tgcgctggca cgtggccttt ccgcaagcgc cgcaggacgc cgccaatcca gacgacaatg
    34861 ccgctggcga cataaatggc cgccatggtg accagcaccg gctttgagaa gttccagatc
    34921 atgtaaatcg tcgctccaag caatacgata ctgagcggcg atctcgggcg catcaggttg
    34981 aggtccttga agctgcggta acgccatgta ctgaccataa gaaacgagag caacgccagc
    35041 aggcagatcc acgcgatacc gagaggccac gacgtaatgg gaacgccgct cgaagcgtac
    35101 accaccgcgc cgaccatggc agcggcggaa ggaataggca gtcccacgaa gtatttcctg
    35161 tccggcctgc ccgggtttcg gggcacggga ttggtctgta cattgaagcg ggcgagccgc
    35221 accgaaccgc aaagcaggaa gagaaacgaa aagaagtacg cgaggttgaa aagattgttg
    35281 cgaatgaacc cgtcctgcgg caggtcgata tattggaacc cccaggtgaa cgccagcacg
    35341 gccggcgcga ttccgaaggt gatcacatcg gcgagagagt ccatttcgcg gccgaaatcg
    35401 ctgaccgtgt tcgtcatccg ggcgatgcgg ccatcgaggc catcgagcgc gaaggcgaca
    35461 ccgatggcga gggccgccac ctcgaagtgg cggttttcac cccaggggag caaggaacct
    35521 tcaaacgatc tcagtatgga cagaaatccc aggaaaatat tgccggcggt gaacaacgtc
    35581 ggaagcgcgt acgcggcccg tctgggctgg cgcccgcccg gtcccgaaac ctgcaaccgg
    35641 tcctgcaatg ggaacctcac cgcgcggaat ctctgcggcg tgcgatgatg ctcgatcccg
    35701 cctgcacacg atccccttca cgcaccgcga tctcccattc ggaaccgaag atcacgtcgg
    35761 tgcgagaccc gaacttgatc atgccgacac gctctccctt cgccactttg tcgccgggct
    35821 tcttgttgaa gacaatccgc cgcgcgatca atccggcgat ctgcttgaac accacggtag
    35881 aaccgccgcc ctcgacggtc accgtattca tttcgttttc agacgatgca atctctttgc
    35941 tcgccacagc gaaccggccc tgctggtatt tcacttgggt gatggcgccc gcgatgggtg
    36001 cgcgattgac atgaacatcg aagacattca gaaagatact gatccggtat gcgcctgctc
    36061 cctgcgcgag cacggccacc actttaccgt cggcgggaga tacggctaca tcgccttcgg
    36121 gaatttgccg gtccgggtcg cggaagaagt aaaggcaaaa cacagcgagg acgtagaatg
    36181 gcaacccata caaaggccgg ccgatccagg aaacaatcag cccgcccgct gtaagcccga
    36241 tcgcatagta gatcccgtcg gcgactatca ttcgtcctaa tcaattgtcg cagcaggggg
    36301 agggttcagc aatcacagca cagagagaag cgccgcgatc attgccgttc tatccgcaat
    36361 gcggtcggtg aggatgcttt cgttcaaagc atgtgcccct tccccaacgc ctccgaggcc
    36421 atcgagcgtg ggaatgccga gcgctgcggt gaaatttccg tccgagccgc cgccggtgct
    36481 ggattcggcg atatccaggc ccacggtccg cccaaggtcc cgggcgtggc ggaacagttc
    36541 gaggccggca cgcgagcgct ccatgggcgg gcggtttact cccccttcga cggtcaggcg
    36601 gcaccgtttg tcgatgggtc gcaacgaccg gaatttgcgt tcgaggccgg ccccgtcctt
    36661 ctggcggacg aaccggaaat cgacatcggc agaggcttca tcggccacca cgttgcttcg
    36721 cgacccgccg acgacgaccc ccggattcac cgtgatgccg cgatcgagat cggtgaaccc
    36781 ggcgatgacg gcgatctggc gggcgagctc gacaatcgcg ctggcgcccg ccgcaaaatc
    36841 gacgccggcg tgagatgccc ggccggcaac gcttacctta aaccctccga tgcctttccg
    36901 tgcggtcttc agcttacctt cgagtccggt gcccggttcc agaaccagca cggcggcgct
    36961 gcgccgcgcc tcccgctccg tgagaggccg cgagctgtcg ctgccaacct cttcgtcggc
    37021 attgagcagc agcacgacgc gccgggaaac agggatatcg agctcccgca gcgcgcgcgc
    37081 cgcctggatg gcgaacgcaa tgcccgattt catgtcgagc acacccggac cccacagacg
    37141 cccgtcacgc tcccggaaag gcatccgggc gagagtgccc aggggccata ccgtgtcgga
    37201 gtggccgagc aacagaagct gctttgtctt gcggcgtccc ggaaggcgaa attcgatgcg
//
LOCUS       DASTMK010000136         9947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_71152, whole genome shotgun sequence.
ACCESSION   DASTMK010000136 DASTMK010000000
VERSION     DASTMK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71152"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFQ79_05660"
     CDS             complement(<1..250)
                     /locus_tag="VFQ79_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU0139172.1"
                     /translation="MGRSAPEKAGTSRKQFMLLEGSPILVHTVRKFFAAPSVTDIFVS
                     LRAEDMSWARELLHAESSPKTVRLVEGGDSRQQSVANAL"
     gene            complement(353..577)
                     /locus_tag="VFQ79_05665"
     CDS             complement(353..577)
                     /locus_tag="VFQ79_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139173.1"
                     /translation="MATKKAVKKTAAKKSGVKKAAVKKVAVKKSTAKKSMPASKKGAK
                     ATKTTASKKATTKKSAAKKPAAKKGGAKKS"
     gene            799..1989
                     /locus_tag="VFQ79_05670"
     CDS             799..1989
                     /locus_tag="VFQ79_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0139174.1"
                     /translation="MNTDRDERICRALDGFEIEIPSWGFANTGTRFGKFLQRAAAATT
                     EEKFADAGQVHAFTGCCPSVAVHVLWDLPKGKDSASEALATAQRYGVRIGSINPNVFQ
                     DQQYKLGSLGNPDAAVRRQALSHLLDCVEIAGVTGSRDISLWFADGSNYPGTANIRRR
                     KKWFEEGLRAAHGALADHQRLLVEYKPFEPAFYHTDIADWCMALLLARVAGPQARVLV
                     DTGHHYQAQNIEQIVAWLLAEDMLGGFHFNDRRYADDDLTLGSIDPYQIFRIFHEIFL
                     FTWETGTTPAIAYMIDQSHNLKPKIEEMIQTAVTAQELYAKASLVDWGSLSRFQDRGA
                     LVDAEECLKAAFAEDVRPAIREWRKLKGLPEDPLAAFRASGYLERAANERGERNQAAL
                     SSYA"
     gene            complement(2004..4700)
                     /locus_tag="VFQ79_05675"
     CDS             complement(2004..4700)
                     /locus_tag="VFQ79_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139175.1"
                     /translation="MRALRRFLSRLHALFTRQQYERRLKEEFEEHLALLTEEKIRAGL
                     SPAEAHRQARLKFGSVEALKDAYRDQRILPRIDALASDIVFGWRQLRKHPAASAASIL
                     SLALAIGAATAAFRLVDAVLLRTLPVAEPERLFFLATKVAGRESGSDYRDQFDYPTFR
                     RYREIIADRADAMLVGIINRRDTVIGAADEPEKLYRQYVSGNLFTVFGLQPALGRLLT
                     PDDDLTPGAHPVAVLSYDCWTSRFARDPGVIGKTFRMEDRHFEIVGVGPKGFIGTEPG
                     EVADVFIPAMMNAEAINHSGWLWFRFWVRPKPGISPEQVLQPLQAALIHERRERLESF
                     HSGTPRQVIDAFLSEKLSLLPAGVGTGRLQHQYRRPLLILSTLVALVLLVACVNVANL
                     MTARTAARAREIALRVSIGAGRWRLVQLVLVESALLAIAGSILGAMFASWAAPLVTSM
                     LRVPGDPVRLVLNTGWRELAFGAALALLVTLLFGLAPALRAAAVRPISALKGTGDPHS
                     RRRLMNALLSAQIAFCILVLFVAGLFVSTFQRLTNRPLGFSPGRLLLIDASATREQPV
                     QTWIQVADHLRQTPGVRSVSLSAWPLLSANRWTGFVRLPGRAIELRQPYFLEVSGGFF
                     ETMGIGLIDGRDFRAGDLPPRLKAPAQPLPGAGIVNETFARVFYNGANPVGRSIDVFQ
                     SKDLSAPMEIVGCVRDAAYANLREPIHPTVYLPMGPRTHTFFLVRTAGDEQALAPVLR
                     REISRVRPDLRIYTIQTQNDSVVWHLVRERLLATLSLFFAIVALALAAVGLYGVLNYS
                     VTRQRREIGIRMALGARVPHVVKGVTANIAVMICLGSAAGVAGSLLGARFLEAFLFEV
                     KATDIGMVVMPVIALTGVALLAALPPAMRAASIDPAETLRSD"
     gene            complement(4697..5044)
                     /locus_tag="VFQ79_05680"
     CDS             complement(4697..5044)
                     /locus_tag="VFQ79_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139176.1"
                     /translation="MKDKSEVQQGTLALMVLKTLEMLGPLHGYGIARRIEQISGDLLV
                     VNQGTLYPVLLKLEQEGAISSEWGVSENNRKARFYRLTRAGRKQLQSETHQWEQTAAI
                     IRRFFTVKAEDLR"
     gene            complement(5171..7900)
                     /locus_tag="VFQ79_05685"
     CDS             complement(5171..7900)
                     /locus_tag="VFQ79_05685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0139177.1"
                     /translation="MKHLSAVLAALYLVSVPANAQEAKIDIPYEKFILDNGLTVLVHE
                     DRKAPIVAVNVWYHVGSKNEKTGKTGFAHLFEHLMFNGTENFNQDYFKVLEKLGATDL
                     NGTTNNDRTNYFQNVPTSALDTVLWMESDRMGHLLGAVTQAKLDEQRGVVQNEKRQGE
                     NQPYGLVYNMITENTYPAGHPYSWTVIGSMEDLNAASLDDVKEWFRTYYGPSNAVIVL
                     AGDIDLKTAKEKAQRYFGAIPPGPPIPKHESWVARREGSHRAILQDRVPQARLYKVWN
                     TPQAYSLDSDLLNLGAAILGQGKTSRLYKRLVYTDQIATDATAFAYARELGGQFIIQV
                     TARPGEDLAKVEKAVDEELRNFLEAGPTDEELQRAKTRYVAGFLRGVERIGGFGGKSD
                     VLANGQVFAGDPGVYRSTLKNRQQATVADIRRVADEWLSDGVFVLEVQPFANYKALSK
                     SDVDRSKVPEPGAPPELRLPKLQRTTLSNGMKVILAERHEIPIVNFQLLVDAGYAADQ
                     FATPGVARLTMTMLDEGTKTRDSIRISEELEDLGAQLGAYSDLDMSHVSLSALKANLD
                     KSLDIYADVILNPVFPEQDFKREQKQQIAAIQREKVQPITMALRVFPALLYGKAHAYG
                     TPLTGSGTEETAAKITRADLVKFHQTWFKPNHSTLVVVGDTTLAEITPKLERIFASWQ
                     RGDIPKKNIAPVEHQKSASVYIVDKPGSIQSMILAGHIAPPKSNPDEIAMETLNTILG
                     GAFISRMNMNLREDKHWSYGAGTVLWNARGQRPFITYAPVQTDKTKESVAEIVKELQG
                     VVKDRPASKDELAMAQDNQTLRLPGSRETMNAVANSIVDLVKYGLPDDYYETYTGKVR
                     ALTTQDISTAAEKLLRPDKVVWIVVGDRSKIEAGLKELNLADVKYIDADGNPL"
     gene            8006..8923
                     /locus_tag="VFQ79_05690"
     CDS             8006..8923
                     /locus_tag="VFQ79_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0139178.1"
                     /translation="MSLVVVGSVAYDGIETLHGRRDRILGGACTYISLAASYFTKPSV
                     VAVVGEDFEQDDYDFLAGHGIDLGGLDRVPGKTFYWSGVYSADMNDRTTLRTDLNVFA
                     DFAPKLPDRYKQSPYLLLGNIQPSLQRSVRAQMNGLRLVGGDTMNYWINDYRQELLDT
                     IREWDFLLINDSEARLLSGESNLRRAACKILEMGPHTLVIKRGEYGAMLFRRDKYFIV
                     PGYLLEDVFDPTGAGDCFAGGFSGYLAQQGLDIHKCDIEPQELHRAVIYGSVMGSFCC
                     EMFGVERFRTLTRQEIDARYQEFKRFTEF"
     gene            8982..>9947
                     /locus_tag="VFQ79_05695"
     CDS             8982..>9947
                     /locus_tag="VFQ79_05695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0139179.1"
                     /translation="MAVALAAISAAAVWWFYSRGHLLDYGDALAHLNIARRIIDSRTP
                     GPEQIGTVWLPLPHLLMLPFVANDFLWRTGLAGSLPAAGCFIAAGLFLFAAARRLFGS
                     NAAGAAAALCFALNPNALYLQSIAMTEAFLFAALLGLLYFAVLFKQTQQFYAVVMAGA
                     ACFAATLVRYEGWFLIPFAAGFFFFAARGRRLAAAIVLGCIASLGPLLWLAHNGYYYS
                     NFLEFYNGPYSAKAIYQRALDGGMARYPGDHEWTKAWLYFRSAAQLCLGWPLVWLGLA
                     GAVAALFKRAWWALALLALPPLFYVLSLYSSGTPIFVPHLWPNSYY"
BASE COUNT         1911 a         2913 c         2979 g         2144 t
ORIGIN      
        1 ccagggcgtt ggcgaccgat tgctggcggc tatcgcctcc ctcaaccagg cgcactgttt
       61 tgggagagga ttcggcatgg agcagttctc ttgcccagct catatcctcc gcgcgcagcg
      121 aaacgaaaat gtccgtaaca ctgggagcag cgaaaaactt gcgaacagta tgcacgagga
      181 tgggagaccc ttcgagaagc atgaactgct tgcggctggt acccgccttc tcgggagcac
      241 ttcgtcccat ccgcgtgcct aggcccgcgg cgggcaagat cacagcgact ttcatggctg
      301 cggccattga aagacgattg tagctgtttt gaggacagta agaacagcca actcagcttt
      361 tcttggctcc gcccttcttg gctgccggct tctttgcagc gctcttcttc gtcgtagctt
      421 tcttcgacgc tgttgtcttg gtggccttag cgcctttctt cgacgcaggc attgacttct
      481 ttgcagtgga cttctttaca gcaactttct taacagcagc cttcttcaca ccggacttct
      541 ttgcagcagt ctttttcacg gctttctttg tagccatcaa gtcctccttc cgaaagtata
      601 accgcgacta catctttatg tctccatttt ttatcgaaat tgttttatac atcacgagtt
      661 gaccgcaaaa acatttaccg cttcgtgcgt ttcaaggtgg tagtactcct tccgaagtgg
      721 atcgtaagcc ggcggaaagc ctctcgcgtt tcgttcttcg cggtgcgata acatacatgt
      781 gctggttgga aagatccgat gaacactgac agagacgagc gcatttgccg ggcgctggac
      841 ggattcgaaa tcgagattcc ttcctggggt tttgcgaaca cgggtacgcg attcggcaag
      901 ttccttcagc gcgcggccgc ggccacaacg gaagagaagt tcgccgacgc cgggcaggta
      961 catgctttca cgggttgctg tccttcggtt gcggtgcatg ttctctggga tctgccgaag
     1021 ggtaaagata gtgcgagcga ggccctcgca acagcacaac ggtacggagt gcggataggg
     1081 tcgatcaatc cgaacgtctt tcaggatcag cagtacaaac taggatcgtt gggtaacccc
     1141 gacgcagcgg tgcgccggca ggcgctttcg cacctcctcg attgtgttga gatcgccggt
     1201 gttaccggca gccgcgatat atccctttgg tttgctgatg ggtcaaatta tccggggacc
     1261 gcgaacatcc ggcggcgcaa gaaatggttc gaagaaggat tgcgtgcggc gcacggggcg
     1321 ctagcggacc atcagcgtct gttagttgag tacaagccgt tcgagccggc tttttaccac
     1381 accgacatcg cggattggtg catggcgctg ctgctggcgc gcgtggccgg tccacaagcg
     1441 cgcgtgctcg ttgataccgg tcatcactat caggctcaga acatcgagca gatcgtggct
     1501 tggctgctcg ccgaagacat gcttggcgga ttccatttca acgaccgccg ttatgcggat
     1561 gacgatctga ctcttggttc catcgatccc taccagatct tccgtatctt tcatgagatc
     1621 tttctgttca cgtgggagac cggaactaca cccgccattg cgtacatgat cgatcaaagc
     1681 cacaacttga agccgaagat cgaagagatg attcagaccg cggttacggc ccaggagcta
     1741 tatgcgaagg catccttggt ggattgggga agcctttcgc ggtttcagga ccgtggcgcg
     1801 ctggtagatg ccgaggagtg tttgaaagca gcctttgccg aggatgtgcg gcccgctatc
     1861 cgggaatggc gcaagctgaa gggactacct gaagacccgc tcgcggcctt tcgcgccagt
     1921 ggatacctgg agcgtgccgc aaatgagcgc ggcgaacgta accaggcggc gctatcgagc
     1981 tacgcgtaag tctctcggcc gcactaatcg ctccgcaacg tctccgcggg gtcgatactc
     2041 gctgctcgca tggccggagg cagcgccgcc agcagagcca cgccggttaa cgcgatcacg
     2101 ggcataacaa ccatcccgat atcggtagcc ttgacctcga acaaaaatgc ttcgaggaaa
     2161 cgggcgccca ataggcttcc cgctacgcct gccgccgagc cgaggcaaat catcaccgcg
     2221 atattggccg tcaccccttt cactacatga gggacgcgag cgccgagcgc cattcgtata
     2281 ccgatctcgc gccgctggcg ggttacagaa tagttgagga cgccataaag tccaacagcc
     2341 gccagcgcaa gcgcgacgat ggcgaagaac aaggacagcg tcgcaagcag ccgttcgcga
     2401 accaggtgcc agaccacgga atcgttctga gtctgaatgg tgtagatgcg gagatcgggc
     2461 cggacacggg aaatttcgcg gcgcagaacg ggtgcgagcg cttgctcatc cccggccgtg
     2521 cgcaccagga aaaaggtgtg ggttcgcggc cccatcggca ggtagaccgt gggatggatc
     2581 ggttcccgca ggttggcata agctgcatcg cgcacacagc cgacgatttc catcggtgcg
     2641 gaaaggtcct tgctttgaaa gacgtcgatg gaccggccca cggggttcgc accgttatag
     2701 aaaacccggg cgaaagtctc attcacaatt ccggctccag gcagaggctg cgcaggggct
     2761 ttcagacgtg gtggaaggtc gccggcccgg aagtcccgtc catcgatcag cccgattccc
     2821 atcgtttcaa agaagcctcc cgatacttcg aggaagtaag gctggcgaag ctcgatggcg
     2881 cggccgggaa gccgtacgaa accggtccag cgattggccg acagcagcgg ccaggcggac
     2941 agcgatacgg atcgcactcc aggcgtttgg cgaaggtgat ccgcaacctg aatccaggtt
     3001 tgcaccggct gttccctggt ggccgaagcg tctataagca gcaaacgccc gggcgagaat
     3061 ccgagggggc ggttcgtcag gcgctggaag gtgctgacga agagtcccgc gacgaacaac
     3121 accaggatgc agaatgcaat ctgagcggac agcagggcgt tcatcaggcg gcggcgcgaa
     3181 tgtggatcgc cggtcccttt cagcgcagag atcgggcgca ccgcggcggc ccggagcgcc
     3241 ggtgcgagtc cgaagagcag agtaaccagt aacgcaagtg cggcaccgaa tgcaagttca
     3301 cgccagcccg tattgagaac cagccggacg ggatcgccgg gtacacgcag catggacgta
     3361 accagtggcg cggcccacga cgcaaacatg gcgccaagga tcgatccggc gatggccagc
     3421 agcgcactct ccaccagcac cagttgaacc aggcgccacc ggcccgcacc gatcgagact
     3481 cggagtgcta tctcgcgtgc gcgagccgca gtccgggccg tcatcagatt ggcgacgttg
     3541 acacaggcga caagaagaac cagggccacc agggtgctca ggatcagcaa agggcgccgg
     3601 tattggtgct gaaggcggcc ggtgccgacg cctgcgggca ggagagagag tttctcactg
     3661 agaaacgcat caatcacctg cctgggagtg ccggagtgga agctctcgag gcgctcccgg
     3721 cgttcatgga tcaacgccgc ttgcaagggc tgtagaacct gctcgggtga gatgcccggc
     3781 ttgggacgaa cccagaagcg aaaccacaac cagccagaat ggttgattgc ttcggcgttc
     3841 atcatagcgg ggatgaaaac atcggccacc tcaccgggct cggttccgat aaaacccttt
     3901 ggcccgacac caacaatttc aaagtggcga tcctccatgc ggaacgtctt tccgatgaca
     3961 ccgggatcgc gggcgaaacg gcttgtccaa cagtcgtagc taaggacggc gaccggatgg
     4021 gcgccgggtg tcaaatcgtc atcgggggtg agtaagcggc caagagccgg ctggagtccg
     4081 aagactgtga agagattgcc ggagacatat tggcggtaaa gcttttccgg ctcatcggca
     4141 gctccgatga cggtgtcgcg ccggttaatg atgccgacga gcatcgcgtc tgcgcgatcg
     4201 gcaatgattt cgcgataccg gcgaaaagtc gggtagtcga actgatcgcg atagtcggag
     4261 ccgctttccc ggccggcaac ctttgtggcg agaaagaaga ggcgctccgg ttccgctacc
     4321 ggcaaggttc gcagcaggac ggcgtcaacc aggcggaatg cggcggtagc ggcgccaatt
     4381 gccagcgcga gcgagaggat ggaagcggca ctggcggccg ggtgcttgcg taactgccgc
     4441 catccgaaaa cgatgtcgga tgccagcgca tcgatccgcg gcaggattcg ttgatcgcgg
     4501 tatgcatcct ttagtgcttc gacggagccg aatttcagcc tggcctggcg gtgtgcctcg
     4561 gccggggaca atccggcgcg gatcttttcc tctgtcagca gcgcgagatg ctcctcgaac
     4621 tcctctttca gccgtcgctc gtattgctgc cgggtgaaca gtgcatggag ccgtgaaaga
     4681 aatcttctga gcgccctcat cgcaggtcct ccgctttcac cgtgaagaag cggcggatga
     4741 tagcggcagt ctgctcccat tgatgggtct cgctttggag ctgcttgcgt ccggcgcgcg
     4801 tcagacgata gaagcgggct ttgcggttgt tctccgaaac gccccattcc gaggagatcg
     4861 cgccttcctg ctcgagcttc agcagcacag gatacagcgt tccctggttg acgacgagca
     4921 ggtctccgct gatctgctcg atgcgccgtg cgatgccgta gccatggagc ggtcccagca
     4981 tctccagggt cttaagaacc atgagcgcca gtgtgccctg ttgtacttcg ctcttgtcct
     5041 tcatgtccgc ccccctccat tgggtaccca acaggaatca tatcgcattt cttttgggaa
     5101 accaacagga gggaggacgg gcgcgcagcc cggtttgaat caggttaccg ggccgcgcgc
     5161 gtgagcaagc ttataacgga ttcccgtccg cgtcgatata cttcacgtcg gccaggttca
     5221 gttctttcaa tccggcctcg attttcgaac gatcgcccac gacgatccac acgactttat
     5281 ccggacgtag caacttttcc gccgccgtcg agatgtcctg cgtcgtcagt gcgcgtactt
     5341 ttcccgtata ggtttcgtag tagtcgtccg gaagtccgta tttcaccagg tccacaatgg
     5401 aattcgcgac ggcgttcatg gtttcacgcg agccgggcag gcgcagcgtc tgattatcct
     5461 gcgccatcgc aagttcgtcc ttcgacgcgg gacgatcctt tacaacgcct tgaagctctt
     5521 tcacaatctc ggccacggat tccttggttt tgtcggtctg caccggtgcg tacgtgatga
     5581 acgggcgctg gcctcgtgcg ttccacagca ccgtaccggc gccgtaggac cagtgtttgt
     5641 cctcgcgaag attcatgttc atacgcgaaa tgaacgcccc gccaagaatc gtattcagcg
     5701 tctccatggc gatctcgtcg gggttcgatt tcggtggtgc gatatgtccg gcgaggatca
     5761 tcgactgaat tgagcccggt ttgtcgacga tgtaaacgct ggcggacttc tggtgctcga
     5821 ccggcgcgat gttcttcttc ggaatatctc cgcgctgcca gcttgcaaag atccgctcga
     5881 gcttgggagt gatttcggcc agcgtcgtat ccccgaccac aaccagcgtg ctgtgattcg
     5941 gcttgaacca ggtctggtgg aatttcacga gatcggcgcg cgtgatcttc gccgcggttt
     6001 cttcggttcc cgacccggtc agcggcgttc cataggcgtg cgctttcccg tacagaagcg
     6061 ccgggaagac tcgcagcgcc atcgtgatag gctgcacctt ctcgcgctgt atggccgcga
     6121 tctgttgctt ctgctcccgc ttgaagtcct gttcgggaaa cacggggttc agaatgacat
     6181 ccgcgtagat gtcgagcgac ttgtccaggt tcgccttcaa cgccgacaac gatacatgcg
     6241 acatatccag gtccgaatac gctccgagtt gcgctcccag atcttccagt tcttcgctga
     6301 tgcgaatgga atcccgggtc ttggtgcctt cgtcgagcat cgtcatggtg agccgggcta
     6361 ctccgggcgt ggcaaactga tcggccgcat agccggcatc caccagcaac tggaagttga
     6421 cgatagggat ctcatgcctt tccgcgagga tcactttcat gccattgctc agcgtagttc
     6481 gctgtaactt cggcagccgc agttcgggcg gcgcaccggg ttcgggaacc ttcgagcggt
     6541 ccacatcgct tttggaaagg gctttatagt tggcaaacgg ctgcacttcc agaacgaaaa
     6601 ctccgtccga gagccactcg tccgcaactc tgcggatgtc ggcaaccgtc gcctgctggc
     6661 ggttcttcag ggtggaccga taaacccccg gatctcccgc gaagacttgc ccgttggcca
     6721 gaacgtcgga tttaccgccg aatccgccga tgcgctcaac gccccggagg aagcccgcga
     6781 cataccgcgt cttggcgcgc tgcaattcct cgtcggtcgg cccggcttca aggaaattcc
     6841 gcaactcctc gtctacggct ttctcgacct tggccaggtc ctcgcccggg cgcgccgtca
     6901 cctgaatgat gaactgtccg ccaagctcgc gcgcataagc gaatgcggtg gcatccgtgg
     6961 cgatttgatc cgtgtatacc agcctcttgt acagacgtga tgtcttgcct tgccccagaa
     7021 tggcggcgcc cagattaagc aggtccgaat cgagcgaata ggcctggggc gtattccaca
     7081 ctttgtagag gcgcgcctgc ggcacgcggt cctggagaat ggcgcggtgg cttccctcgc
     7141 gacgcgcaac ccacgattcg tgtttgggaa taggaggtcc gggcgggatc gcgccaaaat
     7201 acctctgcgc cttctccttc gcggtcttga ggtcgatatc gccggcaaga acgatgaccg
     7261 cgttcgacgg cccgtagtag gtcctgaacc attccttcac gtcgtccagg ctggccgcgt
     7321 tcaggtcctc catggaaccg atgaccgtcc acgagtaagg atggccggcc gggtacgtgt
     7381 tctcggtaat catgttgtac acgagaccat acggctggtt ttcgccctgc ctcttctcgt
     7441 tctgcaccac cccgcgctgt tcatcgagct tggcctgggt gacggcgccg agcagatgtc
     7501 ccatgcggtc ggattccatc cacagcaccg tgtcgagcgc cgacgtaggt acgttctgga
     7561 aatagttggt gcggtcgttg tttgtcgtcc cgttcaggtc ggttgctccg agtttctcca
     7621 gcaccttgaa ataatcctgg ttgaagttct ccgtaccgtt gaacatcagg tgttcgaaca
     7681 ggtgtgcaaa tccggtcttc ccggtctttt cgttcttgga accgacgtga taccagacgt
     7741 tcaccgcgac aatcggcgct ttgcgatcct catgaacgag aacggtcagg ccattgtcca
     7801 gaatgaactt ctcgtaaggg atatcgatct tcgcttcctg cgcgtttgca ggaacggaca
     7861 ccagatacag ggcagccagg acggcggaaa ggtgtttcac gattgcctcc atttgggacg
     7921 cggttcaccg ctacccacca tgccgttaag gtagcacctg cgtaaggctt ccgcaggacg
     7981 gctccggagc cgatagaatc gaggaatgtc actggttgtt gtaggctcgg ttgcctacga
     8041 tggaattgaa accctgcatg gccgccgcga ccggattctg ggcggggcct gcacttacat
     8101 ctcgctcgcc gccagctatt tcaccaaacc cagcgtagtg gccgtggtgg gtgaggattt
     8161 cgaacaggac gactacgact tcctggccgg gcacggtatc gatctcggcg gtctcgatcg
     8221 cgtccctgga aagaccttct actggtccgg agtttattcc gccgacatga acgaccgcac
     8281 taccttgcgg accgacttga acgtgtttgc agatttcgcg cccaagttgc cggaccgtta
     8341 taaacagtcc ccgtacttgc tgttgggcaa tattcagccg tctttgcagc ggtccgtgcg
     8401 ggcacagatg aacggacttc gtctggtggg cggcgacacg atgaactact ggattaacga
     8461 ttaccgccag gagttgctcg acactattcg tgaatgggat tttctgctca tcaacgatag
     8521 tgaagcccgg ctgctctccg gtgaaagcaa cctgcgccgc gccgcttgta agatcctcga
     8581 gatgggtccg cacacgcttg tgatcaagcg cggtgaatat ggcgcgatgc tgtttcgccg
     8641 ggacaagtac ttcattgttc cgggctacct gttggaggac gtattcgacc ccaccggagc
     8701 gggcgattgt ttcgccggcg gattctccgg ataccttgcc cagcaaggcc tggatattca
     8761 caagtgcgat attgaacccc aggaactgca tcgcgcagtg atctatggtt ccgtcatggg
     8821 aagtttctgt tgcgagatgt tcggcgtgga gcgcttccgc accctgacgc gccaggagat
     8881 cgatgctcgt taccaggaat tcaaaagatt cacggaattt tagagccacc gcccggcgcc
     8941 gcggtgaatc cgcgagtctc tccaccgggc cgctggtagc gatggctgta gctcttgccg
     9001 ccatcagcgc agcggcggtg tggtggtttt actcgcgagg gcatctgctc gactatggcg
     9061 atgcgctggc gcatctgaat atcgcccgcc ggattatcga ttcacgaacg ccgggcccgg
     9121 aacagatcgg gacggtatgg ctcccgcttc cccacctgct gatgctgccg ttcgttgcga
     9181 acgatttcct ttggcgcacg gggttggcgg gttccctccc ggcggctggg tgcttcatcg
     9241 ccgcgggtct gttcctgttc gccgcggcgc gccggctgtt cggcagtaat gcggcgggag
     9301 ccgccgcggc gctctgcttt gccctcaacc cgaatgccct gtacctgcaa tcgatcgcga
     9361 tgacggaggc gtttctgttc gcggccctgc tcgggctcct gtattttgca gttttattta
     9421 aacagacgca gcaattctat gcggtcgtga tggccggtgc cgcctgtttc gccgctacgc
     9481 tcgtacgtta tgaaggatgg ttcctgatcc cgtttgccgc ggggttcttc ttttttgccg
     9541 cgcgtggccg gcggctcgcg gccgccatcg tgttgggctg catcgcatca ctaggccccc
     9601 tgctttggct ggcacataac gggtactact acagcaattt cctggagttt tacaacggcc
     9661 cctactccgc caaagcgatc tatcagcgcg cgctggacgg cgggatggcg cgatacccgg
     9721 gcgaccacga atggacaaag gcctggctgt acttccggtc ggccgcgcaa ctgtgccttg
     9781 gctggccgct ggtttggctg gggctggccg gcgctgtggc cgctctgttc aaaagagcgt
     9841 ggtgggcact cgcgttgctg gcactgccac ctttgtttta tgtgttgagt ctctactcct
     9901 ccggcacgcc gattttcgtc cctcacctct ggccgaacag ctactac
//
LOCUS       DASTMK010000137         5838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_103061, whole genome shotgun sequence.
ACCESSION   DASTMK010000137 DASTMK010000000
VERSION     DASTMK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103061"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VFQ79_05700"
     CDS             complement(<1..1483)
                     /locus_tag="VFQ79_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0139180.1"
                     /translation="MPLRTWAAFVPGFGTTLLLALLCSTCLFAQGRRPVVAVAGISHE
                     SASFNPAKTRLEDFGDRASGRELEFLKEAELANTTVSGYVTGAKQYGLDLLPVVVAGA
                     SPKGPVTDDAFNTLAERILAGLKKAPKLDGVLLALHGAMVVESYPSGDLELVRRVRQT
                     VGAQLPVIVTHDFHANVDPEISNYCDVLITYKENPHIDPKERGIQAAKIMAGMISGKV
                     KPVQAIVKPSMMYNIVFQHTRTPPLKPIVDESRRLEQNPKILAVSVSGGYQYADVPQM
                     GPSVIVVTDNDKDLAQTEAKRLADMLWATRDQIRLNLPDAAQAVKQAMASDKAPVVLI
                     DMGDNIGGGSSGDSTFLLEELLKQKAQGWVMVIADREAVKTAFEAGIGKTVEMNVGGK
                     TDNLHGRPVRVRGRVKSMHEGKYTETEIRHGGGRYYDMGYTAVIEVEGSTREFPNLLL
                     VTTARSSPNSLHQLISNGVYPTRQKIIVVKGAIAPRAAYEPVAG"
     gene            complement(1542..2012)
                     /locus_tag="VFQ79_05705"
     CDS             complement(1542..2012)
                     /locus_tag="VFQ79_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139181.1"
                     /translation="MNRWFIRFVCLAILLAFAVMTAQIAGLNGTWKLNVERSRWGKVQ
                     KPVSVVLQIQHAEPHLTYSGNVIYSNEETRTFTFDGAIDGREYHMDRSYGGGSIVIRR
                     LNSQAIKSIFRSTDGRFTETATTTISAGGTVLTRQLQVDSPDGRRSWTEVYNKQ"
     gene            complement(2124..2672)
                     /locus_tag="VFQ79_05710"
     CDS             complement(2124..2672)
                     /locus_tag="VFQ79_05710"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0139182.1"
                     /translation="MDLFIDRMPSPVGELILIWSPGALRVLEFDHDEQRLQRLLRAHY
                     DAYRLTSELAPAELRNPIEAYFRGELSAIDTIPVETGGTPFQRKTWAALREIPAGSTI
                     TYGELAEKIGRRGACRAVGLANGANPVSIVVPCHRVIGTNGALTGYGGGLNRKRWLLE
                     HERAHMPGASQQTTLPGFHTFF"
     gene            complement(2656..3681)
                     /locus_tag="VFQ79_05715"
     CDS             complement(2656..3681)
                     /locus_tag="VFQ79_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0139183.1"
                     /translation="MRRQIWALLMTSFAFSQQTPEAKLLEANRLLEEGEYARAEAVYR
                     DLSGGVSHSALTAKSNLCVVLQRQARYDEASGCTRELLAELTATDIGTRRLRVNVLNN
                     YAAQLLRHRDTLPEAEATILEAIATSAVPPSLSRAEIALPHYTLALIRARQDRIGEAA
                     EQFRDVLKVQRKELGPEHPDVAATLNNLGQIEIRRGDYIEAETLVREALQIWTRRLGP
                     YHPHIAVACNNLAQIYRLSGRRAEAEPFYRRAIEALEHTTGPYHPDLAIMLGNFAGYM
                     IDIGRLGAALSLYERALEAQSRSGGNTADLQRKIAGVTAHLRRQTAGVLVNAQAADYP
                     GRSGSVH"
     gene            3792..4205
                     /locus_tag="VFQ79_05720"
     CDS             3792..4205
                     /locus_tag="VFQ79_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139184.1"
                     /translation="MPSSSSPDAVVQRLTILRNGLLALHKSLLDSERDSYEKDIGRIT
                     SSGQLLNLVLHDPWFAWLRELSELIVLIDEGLAAEQPPVMSDAERFLSQARSLVAPDE
                     NGSGFRKNYFDAMQRDPAIVLAHRDMTKLLELAEK"
     gene            4276..5505
                     /locus_tag="VFQ79_05725"
     CDS             4276..5505
                     /locus_tag="VFQ79_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0139185.1"
                     /translation="MKPLAFLLCAAAAAVAQQPYDLLLKGGHVIDPKNGIDAVRDVAV
                     KDGRIAAVAANIPASDARRVADASSLYVTPGLVDIHVHAFAGSMGREYTGEFCVRPDG
                     FTFRSGVTTVVDAGSSGWRNFGEFRDQIINRSRTRVLAMLNIVGSGMGGRGDVEQNTR
                     DMEPARTAEVAKRHSDVVVGIKIAHYAGPEWIAVDRAIEAGKLAGLPVMVDFATFRPE
                     RPFQDLVLKRLRPGDIYTHAYLSAVPMLDDAGKLLPYLFEAQKRGVIFDVGHGGGSFS
                     FRQAVPAVKQGFLPDSISTDLHVQSMNAGMKDMLNVMSKFLNMGVTLQDVILRSTWNP
                     AREIHREELGHLSVGSAADIAVLRLESGSFGFLDASARRLRGNRKLIAELTVRDGRVV
                     WDLNGLASEDWDRPRTR"
     gene            5512..>5838
                     /locus_tag="VFQ79_05730"
     CDS             5512..>5838
                     /locus_tag="VFQ79_05730"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU0139186.1"
                     /translation="MRLAIGMAVTLVLFASELRIERVFGPEVSTGPYKHPANIDVHRN
                     GDLYLVYYGGAGEYAVDTGVFGSRLRKGETKWSQPKRIAHDPFRSVGNAVVWQAPDGL
                     VWLFYVV"
BASE COUNT         1081 a         1716 c         1800 g         1241 t
ORIGIN      
        1 aaccggcgac cggctcgtag gcggctcgcg gagcaatggc tcccttcacg acgatgatct
       61 tttgccgggt gggatatact ccgttgctga ttaattggtg aagactgttc ggactggatc
      121 gcgcggttgt caccagcagc aggttgggaa attcgcgagt tgagccttct acttcaatga
      181 cggcggtata tcccatatcg tagtagcggc cgccgccgtg ccggatctcg gtttcggtgt
      241 acttgccctc gtgcatcgac ttgacgcggc ctcgcacccg caccggacgc ccgtgcagat
      301 tgtcggtctt tcctccaacg ttcatctcca ccgtctttcc gatgccggct tcgaaagcgg
      361 tcttgacggc ctcccggtcg gcgatgacca tgacccagcc ttgcgccttc tgcttgagga
      421 gttcttccag aaggaacgtg ctgtctcccg acgaaccacc accaatgttg tcgcccatat
      481 cgatgaggac caccggggct ttgtcgctcg ccatcgcctg cttgacggcc tgggcggcat
      541 ccggcaggtt gagcctgatt tgatcgcgcg tcgcccagag catgtcagcg aggcgcttgg
      601 cctcggtttg cgcaagatct ttatcgttat cggttacgac gatgacgctg gggcccatct
      661 gcggcacgtc cgcatactgg tatccaccgg agacgctgac ggcgagaatc ttcggattct
      721 gttccaaacg ccggctctca tccacgatcg gcttcagcgg tggcgtcctg gtgtgttgga
      781 agacgatgtt gtacatcatg gacggtttca cgatggcctg caccggtttg accttgccgg
      841 aaatcatgcc cgccataatt ttcgccgcct gtatgccgcg ttccttgggg tcgatgtggg
      901 gattctcttt ataggtgatc aacacatcgc aatagttgga gatctccggg tccacgttgg
      961 catgaaaatc gtgtgtgacg atgacaggca actgtgcgcc tacggtctgg cgaaccctgc
     1021 ggaccagttc cagatcgccg cttggatagc tctcaacgac catcgcaccg tgcagcgcca
     1081 gaagcactcc atccagcttc ggcgccttct tgagcccggc gagaatgcgt tccgccagcg
     1141 tgttgaacgc atcatccgtc acggggcctt tgggcgaggc gccggcgaca accaccggga
     1201 gcaagtcgag tccgtattgc ttcgcacccg tcacgtaacc gctcacggtt gtgttggcca
     1261 gttcggcttc ctttaaaaac tcgagttcgc gtccggaagc gcggtcgcca aagtcttcga
     1321 gccttgtctt tgccggattg aaggaggcag actcgtgtga aatgccggca acggccacca
     1381 caggccgccg tccttgagca aacaggcacg tcgaacacag gagtgccaac aggagtgtcg
     1441 ttccgaagcc cggcacaaag gccgcccagg tccgtagagg catattgttg ccctagccta
     1501 tcgcggggcg gccaggcttt tcaagcttgg ggtctaccag tttactgttt attgtaaacc
     1561 tcggtccacg accttctgcc gtccgggctg tcgacctgca actgcctggt gagaaccgtt
     1621 ccgcctgccg atatcgtggt tgtggccgtc tcggtgaacc ttccgtctgt ggaacggaat
     1681 atcgacttga tcgcctggga gttgagccgg cggatgacga tgctgccgcc cccgtacgac
     1741 ctgtccatgt gatattcccg tccgtcgatt gcaccgtcaa aagtaaaagt cctcgtctcc
     1801 tcgttggagt agatgacgtt tcccgaatag gtcaagtgag gttcggcatg ttgaatctgt
     1861 aagactacac tgaccggctt ttgcactttg ccccaacgcg atcgctcgac gttaagcttc
     1921 catgttccgt tcaaccccgc aatctgcgcg gtcatgactg cgaacgcgag taggatggcg
     1981 aggcaaacga accgtatgaa ccacctgttc atggttgcct ccgtttctcg aggtgctgaa
     2041 gccgccggag tgcgctggtt catcggcgcc ttgccgggcg gccgacccag acaaagtata
     2101 gcgccggggt gaccccgttt caatcaaaaa aaggtgtgaa accccggaag cgttgtttgc
     2161 tgcgatgcgc ctggcatatg ggcccgctcg tgttccagca gccatcgctt ccggttcagc
     2221 ccgccgccgt aaccggtaag ggcaccattg gttccgatga cgcgatggca cggtacgacg
     2281 atactgacgg gattggctcc attggctaaa cctaccgcgc ggcaggctcc gcgccggccg
     2341 atcttttccg cgagttcgcc ataagtgatc gtgctcccgg cgggtatctc ccgaagcgcc
     2401 gcccatgtct tccgctggaa tggagtgccg cccgtttcaa ccgggatggt atcgatggcc
     2461 gaaagctctc cgcgaaaata cgcctcgatc gggttacgca gttccgcggg cgcaagttca
     2521 gatgtcaggc gatacgcgtc atagtgtgct cgcagaagcc gctgcaggcg ctgctcgtca
     2581 tggtcgaact ccagcacccg cagggcgccg ggcgaccata tcagtatcag ttcgccgact
     2641 ggcgacggca tcctgtcaat gaacagatcc actacgacca ggataatccg ccgcctgagc
     2701 gttgactaac acgcccgcgg tctgccggcg gagatgagcg gtgacgcctg ctattttccg
     2761 ctgaaggtcc gccgtgtttc cacccgagcg cgattgagct tccagggcgc gttcgtagag
     2821 actgagcgcg gccccgagac ggccgatgtc gatcatataa cccgcaaagt taccgagcat
     2881 aatagcgagg tcggggtggt aggggccggt agtgtgctcc agagcttcga tggcgcgccg
     2941 gtaaaagggt tcggcttccg cacgcctgcc ggagaggcga tagatctgcg cgagattgtt
     3001 gcaggcgacg gcgatgtggg gatggtaggg cccgagccgg cgggtccaga tttgaagagc
     3061 ctcccgcacc agagtttcgg cttcgatgta atctccgcgc cggatctcga tctgtcccaa
     3121 attgttcaac gttgcggcaa cgtcggggtg ttcgggcccg agttctttcc tctgaacttt
     3181 caggacgtct ctaaactgtt ccgcggcttc tccgatgcga tcctgccgcg cacgaatgag
     3241 cgcaagcgtg tagtgcggca gggctatctc cgcgcgtgaa agagacggcg gaaccgcgct
     3301 ggttgcgata gcttccagaa tagtggcttc tgcctccgga agggtatccc gatggcgcag
     3361 cagttgcgcc gcatagttgt tcaggacgtt gacgcgaagc cgtcgggttc cgatatcggt
     3421 ggctgtaagc tccgccagca gttcccgggt gcagccggaa gcctcgtcat agcgcgcctg
     3481 ccgctggagg accacacaca ggttgctctt cgccgtcaaa gcggagtgcg agacgccgcc
     3541 ggataagtcc cggtaaacgg cttcggctcg cgcgtattca ccctcttcga gcaggcggtt
     3601 ggcctccagc agcttcgctt ccggcgtctg ctggctgaaa gcgaaagatg tcatcaaaag
     3661 cgcccagatc tgtcgtctca ttgcatcccc tcgcaaccca gaatgagtct tcgttgcggg
     3721 tcgcgggcag tgaaaacagg taggtttacg gtagagtttt acggtaaaag gcgccgcggt
     3781 aggatggggt tatgccgtcc agctcgtcac cagacgcagt tgttcagcgc ctgacaattc
     3841 tgcgcaatgg gcttctggcg ctccacaaat ccctgctcga ttccgagcgc gattcctacg
     3901 agaaggacat cggacgcatc acatcgagcg ggcaattgct gaaccttgta cttcacgacc
     3961 cttggtttgc ctggctgcgt gagctctcgg aactgattgt cctgatcgac gagggcctgg
     4021 cggccgagca gcctccggtc atgagcgatg ccgagcgctt tctttcccag gcgcgaagcc
     4081 tggttgctcc ggacgagaac ggaagcggct tccggaagaa ctatttcgat gcgatgcaac
     4141 gcgacccggc cattgtgctg gcgcaccggg atatgaccaa actgctggag ttggccgaaa
     4201 agtaagaatt gcggcaaccc ccgcgatagc ggagaatggt cgaacgcgga tgctacgcac
     4261 cgcttcgggt agaagatgaa acctcttgct tttctgctat gtgccgcggc cgccgctgtc
     4321 gcgcagcaac cttacgatct gcttctgaag ggtggacacg taattgaccc caagaatggc
     4381 atcgatgccg tccgggacgt ggccgtaaaa gatggccgca tcgccgccgt ggcggcgaac
     4441 atcccggcct ccgacgcgcg tcgcgtggcg gacgcttcct cgctttacgt gacgcccggt
     4501 ctggtagaca ttcatgtcca cgcctttgcc ggctcgatgg gccgggaata cacgggcgag
     4561 ttttgcgtgc gtccggacgg gtttaccttt cgcagcggcg ttaccaccgt cgtggatgcc
     4621 ggcagttccg gctggcggaa ttttggcgag tttcgggatc agatcatcaa ccgttcgcgg
     4681 acacgcgtgc tggcgatgct gaacatcgtc ggaagcggta tgggcgggcg cggcgatgtg
     4741 gagcagaaca ccagggatat ggagcccgcg cgcacggcgg aagtggcgaa gcggcacagc
     4801 gatgtcgtcg tcggcatcaa gatcgcgcac tatgccggcc cggaatggat cgcggtggac
     4861 cgggcgatcg aggcgggtaa gctggccgga ctgcccgtga tggtggactt cgccaccttc
     4921 cggccggaga gacctttcca ggacctggtc ttgaagcgct tgcgccccgg cgacatctat
     4981 acacatgctt acttgtccgc cgttccgatg ctggacgacg ccggaaagct cctgccctac
     5041 ctcttcgagg cgcagaagcg cggcgtgatc ttcgacgtgg ggcacggggg tggcagtttc
     5101 tcattccggc aggcggttcc ggccgtcaag cagggattcc tgccggattc gatttcaacc
     5161 gacctgcacg ttcaaagcat gaacgccggc atgaaagaca tgctgaacgt catgtcgaaa
     5221 ttcctcaaca tgggtgtcac gctccaggat gtcattctgc gaagcacctg gaaccccgcg
     5281 cgcgagattc accgcgagga gcttggacat ctctccgtag ggagcgctgc ggatattgcg
     5341 gtcctgcgcc tcgaatccgg gtcgttcgga tttctcgacg catcggcgcg gcgtttgcgc
     5401 ggcaaccgga aattgattgc cgaactcaca gtacgcgacg gacgcgtggt ttgggatctg
     5461 aacggactgg cgagcgagga ttgggaccgt ccgcggacgc ggtagagtcg catgagactt
     5521 gcaataggaa tggcggtgac gctggtcctg tttgcgagcg agttgcgcat cgaacgtgtt
     5581 ttcggccccg aggtctcgac gggcccttac aagcaccccg ccaatatcga cgtccatcga
     5641 aatggcgatc tctatctcgt ctactacggc ggcgcgggcg agtacgcggt ggacaccggc
     5701 gtgttcggat cgcggcttcg taagggtgaa acgaaatgga gccagccgaa acgaatcgct
     5761 cacgatccgt tccgctccgt tgggaatgct gtcgtgtggc aagctccgga tgggcttgtc
     5821 tggctgttct acgtcgta
//
LOCUS       DASTMK010000138         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_199134, whole genome shotgun sequence.
ACCESSION   DASTMK010000138 DASTMK010000000
VERSION     DASTMK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199134"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1551
                     /locus_tag="VFQ79_05735"
     CDS             433..1551
                     /locus_tag="VFQ79_05735"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0139187.1"
                     /translation="MPLSAIGVPPRVLTALAAALLTLLLPGQVRADCGKCKDIAEKFA
                     REEGFNGTLLVARNGREIYSGAFGTADAEAGTPMTPQTRFEAGSISKWVAAIVTMRLV
                     EQGRLALDEPISTYLPDYRADTGHKLTLRWLMSHSSGVPNQWDAAAKRDPLLKEQSLD
                     NATAVRAYASGDLTFEPGTDWDYSHSNWLIVKAIVERVSGQPYAALVRTLLTVPLGLR
                     DSGTFFGDSINVTGMAVGYASLQPIVTPRRNPVPDFTMLAGGYYTSVGDLQRLMDAVF
                     HHRVLSAASVATLIEVVRPAQSYALGGRIKTLPVAGKPRKVAWEYGSNGAFRVLAWRV
                     LEDGHSVILANNTSFDHMRIGDLATALLDASYEEPASP"
     gene            complement(1595..2200)
                     /locus_tag="VFQ79_05740"
     CDS             complement(1595..2200)
                     /locus_tag="VFQ79_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139188.1"
                     /translation="MAGRDRWIAVLVVVVAQWMLFNLLRTVGPATGQDVESLSLRVRY
                     ITRAEALPPSLKSEVQGTPGIHVSGQRGPAPARLRSDTSTRPETGKDAGQTGKGPLRP
                     LSLELPNSHVPDFSRQVLKGDTRLDRFAAPPERIPMRKPLTGKDVIEGTAQTLGFWPP
                     GYTTDPCPRIRRNIDEWKTDGSAAGRERLEQEMARQARYCP"
     gene            complement(2267..2998)
                     /locus_tag="VFQ79_05745"
     CDS             complement(2267..2998)
                     /locus_tag="VFQ79_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139189.1"
                     /translation="MRRSSEVAANKKAMLVVVALLMGAGYWIFFRGDGGQLAGSTLPM
                     EQEFGQEAAAPLEDVPRAASISRPATPSSLLQRYRCPPGAEDCERNPNVASTPEEAQW
                     LLTHGYPTVKQIQDLRTRSTADYRLEYQQTGSKVAQSLYTMSLAADGEARTAIGLLWQ
                     ATAQGNIYAFHLLSDLYGRTEELANLITSAAYLRAAYLAGDHRAADRYALRFGILSPP
                     ENLLADKEALRIFKDFGQGRSDLRP"
     gene            3497..3676
                     /locus_tag="VFQ79_05750"
     CDS             3497..3676
                     /locus_tag="VFQ79_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139190.1"
                     /translation="MSVLNILPFVLIAVVFLVLRSTKDKMEAGTRLKLLYFSFGYIAH
                     AIVAPFPAWAGKGSS"
     gene            complement(3896..>4158)
                     /locus_tag="VFQ79_05755"
     CDS             complement(3896..>4158)
                     /locus_tag="VFQ79_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139191.1"
                     /translation="NLLHDTRSPFAAYVAGRVMSSGTLGGQPEAIAASFQLARDLGDP
                     RADRFRDAFLDQHPGMDVATVMTAYSYFKSSMERTAADGKGR"
BASE COUNT          764 a         1332 c         1272 g          790 t
ORIGIN      
        1 tgcttggtca gaaacattcg atttctcctt acgtctgggg tgtcgggaat ataacggtct
       61 ttttgccgga gtcatcagca gccgttagtt aacgtttgtg tacgtggatg cgatgtgaag
      121 atgtgtccgc ttccggccag atgcggacgg cacgagccgc cttcccaaaa aaaggccggc
      181 ttttcaggct tctcccgcca gatggagccg aggccgtcgc gacagtgggc cgtgttgggt
      241 ccgccgggct gcggcgatca cagacgactc acggcacggc gcagggcgcc ccccgtaacc
      301 ctccatgcgg tccttctggg cgatggtcat gctccctttc cggtaccgat tcagtgaagg
      361 tcgccggcgg gcaagtaaac cctcaagccc gcagcggcat ccaatggcag tcttcctctt
      421 cttgccgcgc ccatgcccct gtccgccatc ggtgttccgc cccgcgttct gacagctctc
      481 gcggctgccc tgctcaccct gctcctgccc ggccaggttc gcgccgactg cgggaagtgc
      541 aaggacatcg cggagaaatt cgcccgcgag gaaggcttca atggcaccct gctggtcgcg
      601 cgaaacggcc gtgagatcta cagcggcgcc ttcggcacgg cggacgcgga agccggcacg
      661 ccaatgacgc cgcagacgcg cttcgaggcc ggttcgattt ccaagtgggt cgccgccatc
      721 gtgacgatgc ggctggtcga acagggccgc ctggcgctgg acgagccgat ctccacctac
      781 ctgccggact accgggccga taccggccac aagctcacgc tgcgctggct gatgagccat
      841 tccagcggcg tgcccaacca gtgggacgcc gcagccaagc gcgacccgtt gctcaaggag
      901 cagtcactgg acaacgccac ggcggtacgc gcttacgcca gcggcgacct gacgttcgaa
      961 ccgggcaccg actgggacta ttcacactcg aactggttga tcgtcaaggc gattgtcgaa
     1021 cgcgtcagtg gacaacccta tgccgcactg gtgcgaaccc tgctgaccgt cccgttgggg
     1081 ctgcgcgata gcggcacctt cttcggcgac tcgatcaacg tcaccggcat ggccgtgggt
     1141 tatgcctcgt tgcagccgat cgtcacaccc cggcgcaatc cagtgcccga cttcacgatg
     1201 ctggccggcg ggtactacac cagcgtcggt gacttgcagc ggctgatgga tgcggtcttc
     1261 catcatcgcg tcctgtctgc cgccagcgtc gccacgctga tcgaggtggt ccgccccgcg
     1321 caaagctacg cgctgggtgg ccggatcaag acgctgccgg tggcgggaaa gccgcgcaag
     1381 gtggcgtggg aatacggctc caacggcgcg ttccgcgtgc tcgcctggcg ggtgttggaa
     1441 gacggacaca gcgtgatcct ggccaataac accagcttcg accatatgcg catcggcgat
     1501 ctggcgaccg cgctgctgga tgccagctac gaggaaccgg catcgccgtg agggacagtg
     1561 gggtcaccgc gttggcacgg cccctgcgga aggctcaggg gcaataccgc gcttgccgcg
     1621 ccatttcctg ttccagacgc tcgcgacccg ccgcgctgcc atccgtcttc cattcatcga
     1681 tgttgcgacg gatgcgggga cagggatcgg tggtgtaacc cggcggccag aagccgaggg
     1741 tctgggcggt gccttcgatg acgtccttgc cggtcagggg cttgcgcatg ggaatgcgct
     1801 cgggtggagc ggcgaagcgg tccaggcggg tgtcgccttt caggacttgc cgggaaaagt
     1861 cgggaacgtg tgagttcggc agttcaagac tgagcggcct caaaggtcct tttcctgtct
     1921 ggccggcgtc ttttcctgtt tccggtcgtg ttgaagtatc ggaacgaagt ctggctggcg
     1981 caggcccccg ctggcctgac acgtgaatgc caggagtgcc ctgcacttcc gatttcagac
     2041 tgggcgggag cgcttccgca cgcgtgatgt agcgcacccg cagcgataga gactccacgt
     2101 cttgccctgt tgccggcccc accgttctca acagattgaa cagcatccat tgcgccacga
     2161 ccaccacgag gaccgctatc caacggtcac gtcccgccat caatcgcgaa ccttagaaac
     2221 aagccaaggt gccgccgaac agggccgcca aggtggtgcc cggctgtcaa ggacgcaggt
     2281 cactgcgtcc ttgcccgaag tccttgaaga tccggagtgc ttccttgtcg gcaagcaagt
     2341 tctccggagg agacagaatg ccgaaccgga gggcatagcg atctgccgca cggtggtcgc
     2401 ccgccagata agcggcgcgc agatacgctg cgctggtgat caggttggcc aattcctcgg
     2461 tccggccata cagatcggac aataaatgaa aggcatagat gtttccctgt gccgttgcct
     2521 gccaaagcaa accgatcgcc gtccttgcct cgccgtccgc agccagactc atcgtataga
     2581 gtgactgggc gaccttgctc ccggtctgct ggtactccag ccggtagtcc gccgtggagc
     2641 gcgtacggag gtcctgaatc tgctttacgg ttgggtagcc gtgcgtcaac agccactggg
     2701 cttcttccgg agtggaggcc acgttgggat ttcgctcgca gtcttcagcc ccggggggac
     2761 accggtagcg ctgaaggaga ctggatggcg tcgcaggcct cgagatgctc gccgctcgcg
     2821 gcacatcctc caatggagca gccgcttcct gcccgaattc ctgttccatc ggaagggttg
     2881 acccggctag ctgcccgccg tctcctcgaa aaaagatcca gtagccggcc cccatgagca
     2941 aagccaccac gaccagcatc gccttcttgt tggcagccac ttctgatgac ctcctcatat
     3001 cccactacga ccatcgatgt tcgtccggca acatgggcag tgagtacgaa gtggacctgc
     3061 cggaggaagc ccgccccagt gcgaagcaat ccattcaaca actacacatc cagaaacagg
     3121 tggatcacac gcactccgtg cgctagcggt catgtcgttc agggccgtgg atagttgcgt
     3181 ccatgtccga agatcttgaa aagccggaat gcctccttgt cggcgaccag gttttccggt
     3241 ggcgacccgg tacggtcatc ggcaatggat cgacaacaca gacagtgggt acgaagtggg
     3301 cccacaggaa aggtccatca ccataggaat cgtctgaaac atcaggtggc atgcggattc
     3361 aggccatgcg atgcattggc accggcacat gcgtctgtgg cgggttcccc gaccgtgcgc
     3421 tggcggccaa ccgcctctcg tcctatgctc gctctcgccg gttggcgcag ttcagggctt
     3481 cccaaggaca ggaccaatga gcgttttgaa cattctcccc tttgtactga tagcggtcgt
     3541 gtttcttgtc ctccggtcga ccaaggacaa gatggaggcc ggaaccagac tcaagctgct
     3601 ctatttctcg tttgggtaca tcgcccatgc catcgtggcg cccttccccg cctgggcggg
     3661 gaagggctct tcctaacgac gttgcgggcg atgccgcgat ttcagtatca gaatcgctac
     3721 cagcacgcag acagcaccag ccaaacagcg cagcgaatca agcccgccct ctggcggggc
     3781 cacgagtccc agatataggt tccagccgcc ggacgcgaga aatatcagtg ccgccatcct
     3841 cttcaattgc gtggtacgcc gtcgcctatc cacgccgtcg aacaccttcc atcccctacc
     3901 gccctttccc atccgctgct gtcctttcca tgctcgactt gaagtacgag taggcggtca
     3961 tcaccgtcgc gacatccatg ccggggtgct gatcgagaaa cgcatcgcgg aagcggtcgg
     4021 cacgtgggtc tcccagatcc ctggccagct gaaaggatgc ggcgatggct tcgggctggc
     4081 cgcccagcgt tccggaagac atcaccctgc ccgccacgta ggcggcaaag gggctgcgag
     4141 tgtcgtgcag caggttgg
//
LOCUS       DASTMK010000139         9704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_71357, whole genome shotgun sequence.
ACCESSION   DASTMK010000139 DASTMK010000000
VERSION     DASTMK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71357"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..880)
                     /gene="ribA"
                     /locus_tag="VFQ79_05760"
     CDS             complement(269..880)
                     /gene="ribA"
                     /locus_tag="VFQ79_05760"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEU0139192.1"
                     /translation="MSISTFRLTKVADADFPSRFGQFRIYGFEGQRGDTLEEAVVLRM
                     GDMEGDPPLVRIHSQCLTGDVFHSLRCDCRLQLEMALTCIAEEGRGLLIYEHQEGRGI
                     GLLNKLRAYALQDNGADTVEANELLGFKADLRNYELPAEIIRYFGIPAVRLLSNNPDK
                     IQALERAGVQVAERVPCLVEPIQSTEGYLRTKKEKMRHLLEGF"
     gene            1019..2326
                     /locus_tag="VFQ79_05765"
     CDS             1019..2326
                     /locus_tag="VFQ79_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0139193.1"
                     /translation="MVEQELVTLLLKVAVAASLASILIRFSAFKRMLMREERTLVQRL
                     KLAFGFSLIYGSGVATRVVTRSYHAVDLGLEGSFLSGILGGYVTGLISGVIISIPAMA
                     NGEYLSMPLFAGVGVLGGLLRDGAPDKEEVWRFSPFIDLSLYRLIRRWDDHRRTAFHV
                     VFLVAILFAEGVRWAAVEVFGHRAVFSLHVQDAPPLLVATVYATTLFVVTVPLKIWNS
                     ARNELKLDSQQRLLTEARLEALRSQINPHFLFNTLNSVSSLIRTNPEQARVVVYKLSS
                     ILRRLLRKHENLNPLRDELAFIDDYLSIEMVRFGPKLRFLKEIDPETLGWQVPSMILQ
                     PLVENCVRHGISGKVDGGTIRLRTSIVGGKLHLLVEDDGVGIPEAKLATLFERGIGVT
                     NVNQRLKVFFGNDYRMWIDSKPGEGTRTEIEIPSMQPALAAVS"
     gene            2365..3051
                     /locus_tag="VFQ79_05770"
     CDS             2365..3051
                     /locus_tag="VFQ79_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0139194.1"
                     /translation="MDNVQFPLVGWRADGLPKTIADRTRKVYDRVAMVYPVSTFFFHS
                     RAHRHALQASGITDGMRVLEVATGSGEMFRRLVKVNRSGSTVGFDLSPNMAARTQKRV
                     QREFPSSHTICHAVDARYIPFRDEAFDAVVCCYLLELLATDDIYRALNECYRVLRRNG
                     KFALICIGQNTRMFNRVYKVAGKLAPAFWGRQVEQRVPEMIEHVDFDIISDRVVTQTG
                     YPSRVLVARK"
     gene            3126..4301
                     /locus_tag="VFQ79_05775"
     CDS             3126..4301
                     /locus_tag="VFQ79_05775"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139195.1"
                     /translation="MDTDRRDFLRSAALATAPVFIPRKVWGANDRVTYGVIATGGRGR
                     YLNNKFQSLGAQCAALCDVYEPNLELAVKDSPAAKTYVEYRELLERKDLDAVILAGPD
                     HHHCPMMLESLSAGKDIYAEKPLSKTLEESAKMVNAVRKSKQVVQIGMQRRSADAIMK
                     AKQLVDDGALGFVSMVKPQWHWNVSRPLDNSPLPGKIDWTRFLGNAPKRPVTPMRYRR
                     WRYFWDYAGGNMTDQGTHLMDVVQWFMKSGPPKSAVCYGYVGKMEGAEHPDVFSAVFE
                     FEKFMATWTLDYSNSYENGWSITFMGDRGTMILDEKGYRVYAEPWKPENPPTHQEDAP
                     VPVEAHIQNFLDCIKSRKNPNCTVEIAQAAVAGPHLANIALVKGRRMHLGSDMVKVS"
     gene            complement(4306..5082)
                     /locus_tag="VFQ79_05780"
     CDS             complement(4306..5082)
                     /locus_tag="VFQ79_05780"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEU0139196.1"
                     /translation="MSDVLRIADREFRSRLIVGTGKYRSFQEMQRCHEASGTDMVTVA
                     VRRVNLTDKSKESLLDYIDRTKIFILPNTAGCYNADEAIRTARLAREVGLSNWVKLEV
                     IGDPETLFPDNEGLLEATRVLAKEGFVVLPYTNDDLVNAKKLIDAGAAAVMPLAAPIG
                     SGMGVQNAANLRILREKITAVPLVVDAGVGTASDASVAMELGADGVLMNTAIAAANEP
                     EKMALAMKLAIQAGRLAYEAGRMPKKLYATASSPLEGVVR"
     gene            5132..6184
                     /locus_tag="VFQ79_05785"
     CDS             5132..6184
                     /locus_tag="VFQ79_05785"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEU0139197.1"
                     /translation="MKKLSPAYFAMVMATGIVSIAARLLNMPVLASALFYLNAGIYGV
                     LAVLTALRFVRFRREFLADLIDHQRGPGFFTSVAGSCILGSQFVLISGRYEIASALWA
                     TGIVLWVFLTYTIFTAFTIKTSKPPLAEGLTGGWLLAVVATQSIAVLSALIAVHWEQP
                     YRLELNFFALSMWLWGGMLYIWMISLIFYRYTFFKLAPGDLTPPYWINMGAMAISTLA
                     GARLIENSPHAPFLHSLLPFLKGFTVFYWATGTWWIPMLAVLALWRYGYQRFPLRYDP
                     LYWGAVFPLGMYTVCTFEMARTMKLDFLMGLPRYFFFIALAAWMAAFAGFGWRMATGV
                     VRSTGPRRGTSTPLPL"
     gene            complement(6181..7575)
                     /locus_tag="VFQ79_05790"
     CDS             complement(6181..7575)
                     /locus_tag="VFQ79_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0139198.1"
                     /translation="MTAPLIAQTRKRRNVLFIASDDLNECLGAYGHPLVRTPNIDRIA
                     KQGVRFDRAYCQFPLCSPSRTSLMTGLAPDTTTVYNLQRHFREALPDVVTLGQLFQKN
                     GYYTARSGKIYHYGVPGQIGTDGLDDKPSWNEVANPSGVDRRREEPLLTNYTPQRGLG
                     SAISFYASGAKDEEHTDGMVASEAIRMLEQHRGDPFFIAAGFYRPHVPWIAPSKYFDR
                     YPLNSIELIPFDESEMRIAPELAYFTRPAHWGMNEKQRKDAMRAYYASISFMDAQVGH
                     VLDALERLKLADNTIVVFWSDHGYQLGEHGQWMKQTLFEPSARVPLLIGGPGVEARGA
                     GSARTVELLDLYPTLAELCSLKGTPSNLHGCSLAPLVRKPDSRWEKPAVTQVRRARQD
                     GAVMGYSIRTERYRYTMWAGGSEGEELYDYRGDPRELRNRAADTSLASQKRRLRDELD
                     RILRQRGGAEGQAR"
     gene            complement(7725..8981)
                     /locus_tag="VFQ79_05795"
     CDS             complement(7725..8981)
                     /locus_tag="VFQ79_05795"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0139199.1"
                     /translation="MVHIIAGIDIHKRMLMVAAGVMDMHTENGEITEQVAFENRRFGT
                     TDTELRRLAAWLSRFGVQEVVMESTAQYWKPVWLALEGSFRLQLAQAWSNRAPRGKKT
                     DYRDAQRLVRRHFAGGLTLSFVPDAEQRQMRTITRRRTQLTRERVRLRNQLEALLEEG
                     RIKLSSVISDLLGASGRRILEALADGVDNPESLAALGDERLKCTREQLVDALSGNLTA
                     IHRKLVRQYLDQLSLLDQQREELSQMSADSLRDYSEAVRRLAQIPGIRALAAQQIVAE
                     AGPRAEAFPSPAQFSSWIGACPGREESAGENHSSRCPKGNKYLRRVLCQAAQAAVRTR
                     NTRFQQKFRRLVPRLGYSKAIWAIVRHLAVVIWKILHQGATYEERGLPTSPQAAKRRA
                     QRLAKELRALGYSVEIKPMTTELAGV"
     gene            complement(9310..>9704)
                     /locus_tag="VFQ79_05800"
     CDS             complement(9310..>9704)
                     /locus_tag="VFQ79_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139200.1"
                     /translation="SGRDNSFTGIGRDRADFLGGKADLGSDRPHGEMIAQYFDTSLFA
                     FNAIGTFGNSGKNNLRGPKYFNTDLGVLKVTDVTERVSVQFRAEFFNVFNNVNFNAPN
                     ANVSSAQFGRITSALDPRILQFGLKLLF"
BASE COUNT         1844 a         2948 c         2783 g         2129 t
ORIGIN      
        1 tgaggatcaa cgatctcatc cccgatctca tccccgagac gccggaccac gaaaaaaatt
       61 ccgtgcctcc ggtgatctca gccgcctatt tgaacacacg taaagactta acggctgaat
      121 agaatttgga ttccgacgtg ctaaaagcct taccgccatg gaactgatga tcattgggat
      181 ggttcttcca gcgagcttcg ggtttgctta cgcggttcag cggatggcgc ttcggctgtt
      241 tctgcgcgcc ctggagaccc gttagccgtc agaagccttc gagcaggtgc cgcatctttt
      301 cttttttcgt tcgaaggtag ccctcggtcg attggatcgg ctccacaaga cagggcacgc
      361 gttcggcgac ctgtactcct gcccgctcca gcgcttgaat cttatccggg ttattcgaaa
      421 gcagccgcac cgccgggatc ccaaaataac ggatgatctc agccggcagt tcataattcc
      481 tcagatcggc tttgaacccc agcagttcgt tcgcctctac cgtatcggcg ccattatcct
      541 gcaaagcata cgcgcgtaat ttgttgagga gcccgatccc cctgccttcc tggtgctcgt
      601 aaatcaggag gccacggccc tcttccgcaa tacaggtgag ggccatctcc aattgcagac
      661 ggcagtcgca ccgcaaactg tggaacacgt ccccggtgag gcattgcgaa tgaattctga
      721 caagcggagg atcaccttcc atgtccccca tacggagaac cacggcttcc tccagcgtat
      781 cgcctcgctg tccttcaaat ccgtaaatcc tgaactggcc gaaccggctg gggaaatcgg
      841 cgtcagctac tttcgtcagc cggaatgtgg aaatgctcat cgctatcggt gggcagttcg
      901 cgcgagataa gaaatcgtcc ccggctatcg gatggattcc ggccttacgg cctgtggctc
      961 gtctgccctg gtgaccttcc tgtggagcta agtccattat aatgttcagc agccacctat
     1021 ggtggaacag gaactggtca cgctgctgct aaaggtggct gttgccgcat cgctcgcgag
     1081 cattctcatc cgctttagcg ccttcaaacg catgctcatg cgggaggaac gcacgctggt
     1141 gcagcggctg aaactcgcct tcggtttttc cttgatttat ggctccggcg tcgccacacg
     1201 cgtggttacc cgaagctatc atgcggtgga cctcggcctg gagggaagct tcctttccgg
     1261 cattctcggc gggtacgtga ccgggctgat ctccggtgtg attatctcga tcccggcgat
     1321 ggcaaacggc gaatatcttt ccatgccgct gtttgcgggt gtcggggtgc ttggcggcct
     1381 gctgcgggac ggcgctcccg acaaagagga ggtctggcgg ttttcgccgt ttatcgactt
     1441 gagtctctac cgcctcatcc gccgctggga cgaccaccgc cgcactgcct ttcatgtcgt
     1501 attcctggtc gccatcctct ttgcggaagg cgtccggtgg gccgccgtcg aggtgtttgg
     1561 tcatcgagcc gtcttttccc tgcacgtgca ggatgcccca cctctgctcg tggcaaccgt
     1621 gtatgcgacc acgctttttg tagtaacggt gccgctgaag atctggaaca gcgctcgaaa
     1681 cgagctgaaa ctggactcgc agcagcgcct gctcacggaa gcgcggctgg aggccctgcg
     1741 gagccagatc aatccgcact tcctctttaa caccttgaat tccgtctcct cgttgattcg
     1801 tactaatccg gaacaggcgc gagtggtggt ctacaagctc tccagtattc tcaggaggct
     1861 gctcaggaaa catgagaacc tgaacccgct tcgagacgaa ctcgcgttca tcgacgatta
     1921 tctttccatc gaaatggtcc ggttcgggcc gaagttgcga tttttgaaag aaatagatcc
     1981 ggagacgctc ggatggcagg tgccgagcat gattctccag ccgctggtgg aaaactgcgt
     2041 ccgccatggc attagcggta aggttgatgg tggcacgatc cgcctgcgaa catccatagt
     2101 tggaggaaag cttcatctgc tggtggaaga cgacggcgtc ggcatcccgg aagcgaaact
     2161 ggcgactttg ttcgaacggg gaatcggcgt cacgaacgta aaccaacgcc tgaaagtatt
     2221 cttcggaaac gattacagga tgtggatcga cagcaagcct ggcgaaggca cccggactga
     2281 aattgaaatc cccagtatgc agcctgccct cgccgcagta tcctaggagt ttgtttgata
     2341 cctactttat acagggagca tagattggat aacgttcaat tcccgctcgt gggctggcgc
     2401 gcagacggat tgcccaaaac cattgcggac cgtacgcgga aggtctacga ccgggtcgcg
     2461 atggtttatc ccgtctctac gttctttttc cattcccggg cacaccggca cgccctccag
     2521 gcttcgggta ttacggatgg catgagggtg ctcgaagttg ccaccgggtc cggcgagatg
     2581 ttccgccgcc tggtgaaagt gaaccggtcc gggtcgacgg tgggtttcga tctctcgccc
     2641 aacatggccg cacgcaccca gaagcgcgtc cagcgtgaat tcccgtcgtc ccacaccatc
     2701 tgccacgcgg tggatgcccg ctacatccca ttccgggatg aggcctttga tgccgtcgtt
     2761 tgttgttatc tgctcgagtt gctggccacc gacgatatct atcgggcttt gaacgagtgc
     2821 taccgcgtac tccgccgcaa tggcaagttc gcgctgatct gcatcggcca gaatacacgc
     2881 atgttcaacc gtgtttataa ggttgccggc aaactggctc cggcattctg gggccggcaa
     2941 gtggagcagc gcgttccgga gatgatcgaa catgtcgact ttgacatcat ctccgaccgt
     3001 gtagttaccc agacaggcta cccctcacgc gtactggtcg cccggaagta gcacggcccg
     3061 cctattcctg atagagtatt ttctgccggc gcccgcgtgg cgcctccaat ctacaggagt
     3121 tcgcaatgga taccgatcgc cgggattttc tccgaagcgc agcactggca accgcgcccg
     3181 tctttattcc acgaaaggtc tggggcgcca acgatcgcgt cacttatggc gtcatcgcca
     3241 cgggcgggcg gggccgttat cttaacaata aatttcagtc gttgggcgct cagtgcgcgg
     3301 cgttatgcga cgtgtacgag cccaacctgg aactggcggt gaaggactca cccgcggcca
     3361 aaacctatgt agagtaccgc gagttgctcg agcgcaagga tctggacgcg gtgatcctgg
     3421 ccggcccgga ccatcatcat tgcccgatga tgctcgagtc gctctccgca ggaaaggaca
     3481 tctacgcgga aaagccgctc tcgaagacgc tcgaagagag cgcgaagatg gtgaatgccg
     3541 ttcgcaagtc gaaacaggtg gtgcagatcg gaatgcagcg ccgcagcgcg gacgccatca
     3601 tgaaggccaa gcaactggtg gacgacgggg ccctcggttt tgtctccatg gtgaagcccc
     3661 agtggcattg gaacgtgtcc aggccgcttg acaattcccc gctgcccggc aaaatcgatt
     3721 ggacgcgatt ccttggcaac gctcccaagc gcccggtgac gccgatgcgc taccggcgct
     3781 ggcgctattt ctgggattac gcaggcggga acatgaccga ccagggcacc cacctgatgg
     3841 acgtggtcca gtggttcatg aaatcggggc caccgaaatc cgcggtctgc tacggttacg
     3901 tcggcaagat ggaaggcgcc gaacatcccg atgttttctc ggcggtcttc gaattcgaga
     3961 agttcatggc cacctggacg ctcgactaca gcaattccta tgagaacggc tggtccatca
     4021 cgttcatggg cgaccggggc accatgattc tcgatgagaa gggttaccgc gtgtacgccg
     4081 agccatggaa gccggagaat ccgcccacgc accaggagga tgcccccgtt ccggtcgaag
     4141 cgcacattca gaatttcctg gattgcatca aatcgcgcaa aaatcccaat tgcacagtcg
     4201 aaatagcaca ggcggctgtt gcgggaccgc acctggcgaa catcgccctg gtgaagggac
     4261 gccgcatgca cctgggtagc gatatggtga aggtgtcctg aagatctacc ggactactcc
     4321 ctcgagcggg ctgctggccg tggcgtaaag tttttttggc atgcggcccg cttcgtaagc
     4381 caggcgtccc gcctgtatcg cgagcttcat cgccagtgcc atcttttcag gctcatttgc
     4441 cgccgcgatg gccgtgttca tcagcacacc atccgccccg agctccatcg caacggaagc
     4501 gtccgacgcg gttcctacac ctgcatcgac aaccagcggc accgccgtga tcttttcgcg
     4561 cagaatgcgc aggttcgcgg cattttgcac tcccatgccg gacccgatgg gcgcggccag
     4621 tggcattacc gccgccgctc cagcgtcgat caatttcttt gcgttcacca ggtcgtcatt
     4681 ggtatagggc agcaccacaa aaccctcttt cgccagaacg cgcgtcgctt ccagcagccc
     4741 ttcattgtcg gggaacaggg tctccggatc gccgattact tcgagcttca cccaattgga
     4801 aagccctact tcacgcgcca gccgcgccgt gcggattgcc tcgtctgcgt tataacagcc
     4861 ggccgtattc ggcagaatga atatcttcgt gcgatcgatg tagtccagca gagattcttt
     4921 tgatttatcg gtcaggttca cgcggcgcac ggccactgtg accatgtccg ttcccgaggc
     4981 ctcgtggcag cgctgcattt cctggaagct gcgatactta ccggtgccta cgatcaagcg
     5041 cgagcggaac tcacgatcgg caattcttaa tacatctgac atatggtttc attgtagctg
     5101 cggcacgggc tgccggattg catcacaccc tatgaagaag ctgtctcccg cctacttcgc
     5161 gatggtcatg gccacaggca tcgtgtccat tgcggcccgg cttctgaaca tgccggtact
     5221 tgcgagtgct cttttctact tgaatgccgg catttatggg gtgctggccg tgctgaccgc
     5281 tctgcgtttc gttcgcttcc gccgcgagtt tttggccgac ctgatcgatc accagcgggg
     5341 tccgggattc ttcacctccg tagcgggctc ctgtatcctg ggcagccagt tcgtgttgat
     5401 ttccggccgg tatgaaatag cgagcgccct ttgggccacc ggcatcgtgc tgtgggtgtt
     5461 cctcacctac acgatattta cggcgttcac gatcaaaacc agtaagccgc cactggccga
     5521 gggactgacc ggaggctggc tgctggccgt ggtggcaacg cagtcgattg cagttctgag
     5581 cgcgctcatt gcggtccatt gggaacaacc ctacaggctg gagctcaact tcttcgcact
     5641 gtcgatgtgg ctgtggggcg gaatgcttta tatctggatg atctcgctca tcttctaccg
     5701 gtatactttc ttcaagctcg ctcccgggga cctgacgccg ccgtattgga tcaatatggg
     5761 ggcgatggcg atttcgacgc tggcgggcgc ccgcctgata gagaactcgc ctcatgcgcc
     5821 gttccttcac tcgctgctgc cgtttctgaa ggggttcacg gtcttctatt gggccacggg
     5881 gacatggtgg attcccatgc tcgccgttct ggcgctgtgg aggtacggct accagcgctt
     5941 cccactccgg tacgatcctc tgtactgggg cgccgtgttt cctctgggca tgtacacggt
     6001 ttgtacgttc gagatggcgc gaacgatgaa gctggatttc cttatggggc tcccccgcta
     6061 cttctttttc atcgcccttg cggcgtggat ggcggctttt gcgggattcg gatggcggat
     6121 ggcgactgga gtggttcgtt ctaccgggcc gcggcgcggc acttccaccc cattaccgct
     6181 ttagcgagcc tggccctctg ctccgccgcg ttggcgtaag atacgatcaa gctcatcgcg
     6241 gagccggcgc ttttgcgatg ccagcgacgt atcggccgcc cggttgcgca attcccgggg
     6301 atcgccccga taatcatata gctcctcccc ttccgagccg ccggcccaca tggtgtagcg
     6361 gtaacgttcg gttcgaatcg agtagcccat caccgcccca tcctgccggg cgcgccggac
     6421 ctgtgtgacg gcaggcttct cccagcggct atccggtttt cgaacgagcg gcgcgagact
     6481 gcacccgtgc aggttggagg gtgtcccttt gagcgagcac aactccgcca aggttgggta
     6541 aagatcgagc aactccacgg tacgcgccga gccggcgcca cgcgcttcaa caccgggccc
     6601 accgatcaac agtggcacac gggcggaagg ctcgaacagc gtctgtttca tccattgtcc
     6661 gtgttcaccg agctggtaac catggtcgct ccagaacacg acgatcgtgt tgtccgccag
     6721 ttttaaccgt tcgagcgcat ccagaacatg gccgacctga gcgtccatga aggagatgga
     6781 agcgtaatag gcgcgcatgg cgtccttacg ctgcttctca ttcattcccc agtgcgcggg
     6841 tctggtgaag taagcaagct ccggtgcgat ccgcatctcg ctctcatcga acggaatgag
     6901 ctcgatgcta ttcagcggat agcggtcgaa atacttcgat ggagcaatcc acggtacatg
     6961 ggggcggtag aacccggccg ctataaaaaa cggatcgccc ctatgctgtt cgagcattcg
     7021 aatcgcttca ctggcgacca tgccgtcggt gtgctcttcg tccttcgccc ccgatgcata
     7081 gaacgatatc gcagacccga gtccccgctg cggagtgtag tttgtcagca gaggctcttc
     7141 ccgccggcgg tccactccac ttgggttcgc cacttcgttc cacgaaggct tgtcatccag
     7201 gccgtccgtt ccgatctgcc ccggaacgcc gtaatggtaa atcttgcccg agcgcgccgt
     7261 gtagtatccg ttcttctgaa agagctggcc cagcgtcacc acgtccggca atgcctcccg
     7321 gaaatgacgc tggaggttgt agaccgtggt cgtatcgggt gcgagcccgg tcatcagcga
     7381 cgtacgcgac ggactgcaca gcgggaactg gcagtaagcg cggtcgaacc gcactccctg
     7441 cttcgcgatg cgatcgatgt tcggagtgcg aaccagcggg tgcccatagg ctccgaggca
     7501 ttcgttcaaa tcgtcggagg cgataaacag aacattgcgg cgcttgcggg tctgcgcaat
     7561 cagcggagcg gtcattgcgc ctgcagtaac gaaaaactcc ctgcggctga gatctggcat
     7621 agtaaaccgg ccgggttccc tccccggccg gatgccacat tctatcgcgc ccggctgctt
     7681 agcaggatgc tgaaaaagga gttcggggtc cgctccgtcg aaagtcatac cccggcaagc
     7741 tcggttgtca tgggtttgat ctcgacggaa taaccgagag ctcgcaattc tttggcgagt
     7801 ctctgggcgc gtcgtttggc ggcctgtggc gaggtgggta gtccgcgttc ctcgtaagtg
     7861 gcgccctggt ggagaatttt ccagatcact accgcgaggt ggcggacgat ggcccagatg
     7921 gcctttgagt atccgagtct tggtacaagg cggcgaaatt tctgttggaa gcgggtgttg
     7981 cgggtgcgga ctgccgcctg ggccgcctga catagaacgc gtcggagata cttgttgcct
     8041 ttcggacagc ggctgctgtg gttctctccg gcgctttctt cacggcctgg acaagcgccg
     8101 atccaggaac tgaactgtgc ggggctggga aaagcctcgg cacgcgggcc agcttccgcc
     8161 acgatctgct gtgcggccag agcgcggatg ccgggaatct gtgccagtcg cctcaccgcc
     8221 tcgctgtagt cgcgcaggga gtcggcggac atttgcgaca gttcctccct ctgctggtcg
     8281 agcagcgaaa gctgatccag atattgccgc accagcttac gatgaatcgc cgtgaggttc
     8341 ccgctcagcg catcgaccag ttgttcccgt gtacacttca accgctcatc gcccagggcg
     8401 gccagcgact ccggattgtc caccccgtcg gccaacgcct ccagtattct tcttccgctg
     8461 gcccccagca ggtcactgat cacgctggat aacttgatcc gcccttcttc cagcagcgcc
     8521 tccagttggt tccgcagccg cacacgctcg cgcgtcaact gggtacgccg gcgtgtgatc
     8581 gtccgcattt gccgctgttc ggcatccgga acaaagctta gcgtcagtcc cccggcaaaa
     8641 tgacggcgca ccagccgctg cgcatccctg tagtccgtct tcttgccacg cggcgcccga
     8701 ttgctccaag cttgggccaa ctgcaagcgg aaacttccct ccagcgccag ccacaccggc
     8761 ttccagtatt gcgctgtgct ctccattacc acttcctgca caccgaatct actcagccac
     8821 gcagccaagc gccgcaactc ggtgtccgtc gtaccaaacc ggcggttctc gaaggcaacc
     8881 tgttcggtga tttcgccgtt ttcggtgtgc atgtccatca ccccggcggc caccatcagc
     8941 atccgtttgt gaatgtcaat gccagctata atatgaacca cagtatcctc cagcgtggaa
     9001 gcggagaaca cgtaatggtt ttaaacgcac cgtcccgtgc taccgcttcc ccgctggagg
     9061 ttgcagcgca cattcattgg ctctcgcacg tattccgggt caaactccct aacggcttca
     9121 actgatccag agcggaaccg acctcgaccg ctccacgcca ttcactaagc tactccttcg
     9181 gcgacaagac ctgcccagac tttcatcgcc ttgggcggcg cgaagcgcca tggcaactcc
     9241 ctcgcggtca cggctcggtt ccgagccgcg tgcgccagca agcggttttt gccctttttc
     9301 agcaggctct caaaacagca gcttcaagcc gaactgcaaa atgcgcggat cgagcgccga
     9361 ggtgatcctg ccgaattgcg ctgacgacac attggcgttc ggggcgttga agttcacgtt
     9421 gttgaacacg ttgaagaact ccgcccggaa ctgtaccgat acccgctcgg tcacatccgt
     9481 tacctttagc acaccgagat cggtattgaa gtatttggga ccgcgcaggt tgtttttacc
     9541 ggaattgccg aaggtcccga tcgcgttgaa ggcgaagagc gaggtatcga agtactgcgc
     9601 gatcatttca ccgtgcggcc ggtccgagcc caggtccgct tttccgccca ggaaatcggc
     9661 gcggtcgcgg ccaatgccgg taaaggaatt atcgcggccg ctgg
//
LOCUS       DASTMK010000140         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_550640, whole genome shotgun sequence.
ACCESSION   DASTMK010000140 DASTMK010000000
VERSION     DASTMK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550640"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFQ79_05805"
     CDS             complement(<1..802)
                     /locus_tag="VFQ79_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0139201.1"
                     /translation="MEIFRNYVNGEWIAGETFENRNPANTDEVVGLFVKGNAGNITEA
                     AEAAAAALPAWSSMPAPARGNYLFKVADILEREFDQIAREMTCEEGKTLPEAKGEVRR
                     SINIFRYFAGEGSRLPGFLVPSERERVHMFAVRKAIGVVGLITPWNFPSAIPAWKLAP
                     ALICGNTAVVKPASVAPLSAWRIVEACHQAGIPKGVVNFVAGSGGELGGALLRAAPIK
                     GVSFTGSCSVGDWVHQEASKRRLRIQLEMGGKNPTIVLADADFPAAVEN"
     gene            complement(861..1427)
                     /locus_tag="VFQ79_05810"
     CDS             complement(861..1427)
                     /locus_tag="VFQ79_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU0139202.1"
                     /translation="MKILLIVIGILVVIALFFGTQMVGVRNDLVTQRESIKGAWSQVD
                     VAIQRRADLIPNLVETVKGFAAQEKEVLQSIANARSALINARNPQERIQANSQLDSAL
                     GRLLVVVENYPTLKSNENFLRLQDELAGTENRIAVERRKYNETVQKYNTTIELFPNNI
                     AATLFGFERNDAYFQTEPASRQVPAVKF"
     gene            complement(1430..2140)
                     /locus_tag="VFQ79_05815"
     CDS             complement(1430..2140)
                     /locus_tag="VFQ79_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139203.1"
                     /translation="MERVLNQLIEKLKKAHGDILVSTVLYGSAATGDYQGGFSDLNIL
                     CVLRQVTPSELQHSEPIFRWWRELGNPSPLLMSTEEVRTSTDSFPIEFYDIKARHRIL
                     YGEDVVVNLDIHFDHHRAQVEHELRAKVLRLRQKAAGVLSDKALLLQLMADSISTFCT
                     LFRHVLLLAGEEPRWQKRDVVAAAAGRFGFDPDPFYTLLDLRDEKRKARHIDPVPLLD
                     TYLRQLVIVVEAVDRLER"
     gene            complement(2146..>2822)
                     /locus_tag="VFQ79_05820"
     CDS             complement(2146..>2822)
                     /locus_tag="VFQ79_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU0139204.1"
                     /translation="AGARVQVEQYLTKVEQATGAEIAIVTLPTLAGEPVEDVANALFR
                     KWGIGKKGKDEGILLLLVTQDRRSRLEVGYGLEPLIPDGFTGSVLREMRPALRENQYG
                     DAVMAAAQVLGSRIAQAKGVSLDVNLRPRRAPRSDPFPWPIVLGGIFLLFWILGAGGR
                     GRGGRGGGRGIGGGFLPGMIIGNMMGRSLGGGFSGGGFGGYDSGGGFGGFGGGDSGGG
                     GASSNW"
BASE COUNT          577 a          896 c          788 g          561 t
ORIGIN      
        1 cattttcgac ggcggcaggg aagtcggcgt cggccaggac gatggtcggg ttcttgcctc
       61 ccatttccag ttggatgcgg aggcgccgct tcgaggcctc ctggtgcacc caatcgccca
      121 cagagcagga gccggtgaac gacacaccct tgatgggagc ggcgcggagc agcgcgccac
      181 ccaactcccc tcccgaaccg gcgacaaagt ttaccactcc cttcggaatt cccgcctggt
      241 ggcacgcctc cacaattcgc caggcgctga gcggcgcaac ggacgcgggt ttcacaacag
      301 ccgtattgcc gcaaatgagc gccggcgcaa gtttccaggc cggaatggca ctcggaaaat
      361 tccagggcgt aatcagtccg acaaccccaa tggccttgcg aacggcgaac atgtgaacgc
      421 gctcgcgctc tgaggggacc aggaaacccg gcaggcgcga accttcaccg gcgaagtacc
      481 ggaagatgtt gatgctgcgg cgcacttcgc ccttcgcttc cggcagcgtc ttaccttctt
      541 cacacgtcat ttcgcgcgcg atctggtcga actcccgttc gaggatgtcg gcgactttga
      601 agagataatt tccgcgcgcc ggggccggca tcgacgacca ggcgggcagg gccgccgcag
      661 ccgcttcggc ggcttccgtg atattgccgg cgttgccctt cacaaacagc ccgactactt
      721 cgtcagtgtt ggcaggattg cgattctcga aagtctcgcc tgcgatccat tcgccattta
      781 cgtaattccg aaatatctcc atatttcaaa aaacccgtct gccttttgtg cgtgccgctc
      841 gattggcgcc ggaggaaaga tcagaatttc acagcgggca cctgccgcga tgccggctcg
      901 gtctggaaat aagcgtcatt gcgctcgaaa ccgaagagag tcgcggcgat gttattgggg
      961 aatagttcga tggtcgtgtt gtacttctga acggtttcgt tgtacttccg ccgttccacg
     1021 gcgatacggt tttcggtgcc ggcgagttca tcctgcaagc gcaggaagtt ctcattggat
     1081 ttgagggtgg gataattctc caccaccacc agcagccgtc cgagagcact gtcgagttgc
     1141 gagttcgcct gaatacgctc ctgtggattg cgggcattga tcagagcgga tcttgcgttg
     1201 gcgatggatt gcagcacttc cttttcctgc gcggcgaatc ccttcacggt ctccacgagg
     1261 ttgggaatca ggtccgcgcg gcgctgaatg gccacgtcca cctgcgacca ggcgcccttg
     1321 atgctttccc gttgggtaac gaggtcgttc cgtacgccca ccatctgcgt tccgaagaag
     1381 agagcaatca ctaccagaat cccgataaca atcagaagga tcttcattct tacctttcca
     1441 gtctatccac ggcttccacc acgataacga gctgtcgcag gtacgtgtcc agaagaggaa
     1501 ccgggtcgat gtgccgcgct ttccgcttct catcgcggag atcaagaaga gtatagaacg
     1561 gatcggggtc gaatccgaac cggccggccg ccgctgctac cacatcgcgc ttctgccacc
     1621 ggggttcttc gcccgccaat aacaacacat gccggaacaa cgtgcagaac gtagagatgg
     1681 aatcagccat caactgcagc agaagcgctt tatcggaaag cacacctgcc gccttctggc
     1741 gcaatcgtag cacttttgcc cggagttcgt gttccacttg cgcgcgatga tggtcgaaat
     1801 ggatgtccag gttcacgact acatcttcac cgtaaagaat ccggtgacgc gccttgatat
     1861 cgtaaaactc gatggggaag ctgtcggtgg acgtccgaac ctcttcggtg ctcatcaaca
     1921 gcgggctggg gttgcccagc tcacgccacc aacgaaagat cggctcggaa tgttggagct
     1981 ctgacggagt cacctggcgc aacacgcaaa ggatgttgag gtcggagaat ccgccctgat
     2041 agtcgcccgt cgcggcggac ccgtaaagga cagtggaaac cagaatatcc ccgtgggcct
     2101 ttttgagctt ctcaatcagt tggttcagta cccgttccat gacacctacc agttgctgga
     2161 cgcgcctccg ccacccgaat cgccgccgcc aaaaccgcca aaaccgcctc cggaatcgta
     2221 gccgccgaac cctccgccac tgaagccgcc acccagggaa cggcccatca tatttccgat
     2281 gatcatgccg ggaagaaagc cgccaccgat gccccttcca cctccgcggc cgccacgtcc
     2341 acgcccgccg gcaccgagaa tccagaacaa cagaaatata ccgccgagga caatcggcca
     2401 gggaaaagga tccgagcgcg gcgcgcggcg cggccggagg tttacatcga ggctgacgcc
     2461 cttggcctgc gcgattctcg aaccaagaac ctgggccgcc gccataacgg catctccgta
     2521 ttggttctcc cgcagcgcgg gccgcatctc ccgcagcacg ctcccggtga aaccatccgg
     2581 gatcagtggc tccaggccat atcccacttc cagcctgctg cgccggtcct gtgtgactag
     2641 cagcagcaga atgccttcat ccttgccctt ctttccgata ccccacttac ggaacagcgc
     2701 attggcaaca tcctcgacgg gctcgcccgc cagcgttggc agggtcacga tggcgatttc
     2761 ggctcctgtc gcctgttcga ctttggtgag atactgctct acctgaacgc gcgctccggc
     2821 gt
//
LOCUS       DASTMK010000141        21479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_295691, whole genome shotgun sequence.
ACCESSION   DASTMK010000141 DASTMK010000000
VERSION     DASTMK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21479
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295691"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..623
                     /locus_tag="VFQ79_05825"
     CDS             138..623
                     /locus_tag="VFQ79_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEU0139205.1"
                     /translation="MAVDLYLKIDGIEGESMDSKHGKEIEVESFSWGETQSGTSTHGA
                     GMGAGKVNMQDFHFVMRVNKSSPKLMLACASGEHIKSAILTCRKAGKDQQEFLKITFS
                     DLLVSSYQTGAASGVDSIPMDQISLNYSKIEYEYKEQKADGTLAGAIKTGWDLKLNKK
                     V"
     gene            642..1355
                     /locus_tag="VFQ79_05830"
     CDS             642..1355
                     /locus_tag="VFQ79_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEU0139206.1"
                     /translation="MTARELFQAGRLNESVQALGAELRKDPTDTRRRTFLFELLCFAG
                     EYERAEKQLDILASEKKESDLGALLYRAAIHAERTRNEMFSKRDFPSGPASEQVDGTF
                     EGQPFSTIEDGDPRIGPRLEVFAAGAYLWIPFEHLASIEIPEPKRLRDLIWTPALVRT
                     GPAFKGKELGEVLLPVLTPLSSRHPDDNVRLGRSTIWEETEDGRVVPAGQKMIVVDGE
                     EVPILEIRSIHFVATQAAA"
     gene            1365..3200
                     /gene="tssA"
                     /locus_tag="VFQ79_05835"
     CDS             1365..3200
                     /gene="tssA"
                     /locus_tag="VFQ79_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEU0139207.1"
                     /translation="MRDDLLTPIPGANPGGENLRYSTIVDKIKEARREEDDAPQGEWQ
                     RERKVADYVTVIKLAGDAIAAKSKDLQLATWLTEALLKREGIAGLRSGLQLIKSLLEN
                     FWDHLYPELEDGDPEMRAVPLEWIGTKLDDAVRKAPLTKSGYDWYSYKDSRAVGYEEA
                     CESDSAKLEARALKIADGKLTPEEFDKAFTATPKDFYAKTTEDLEATLVLLEELSALG
                     EEKFGDVAPGYGRLKTAIEEVQNTVRILLQKKREVEPDDEPAVEEQPEEPSFIEETVT
                     AGESGSETAAAPVRAPVRTAKKVTSIEPVDRGDAVDRVTAAARFLRQQDVYDPVPYLM
                     IRGLRWGELRTSETIDATLLVPPATEMRQRIKQASLEGNWEAVLNAAEEAMAMPCGRG
                     WLDLQRYVVRACQELGSYYDRIAAAVKSSLRELLADYPHLPEMTLMDDTPVANPETQQ
                     WLRGEIVPVSPSPFSTAITATPIRSGPEDSAEENAEPKPPDAFEIAMQAVHNGHVQEA
                     IEILMREAAQEQSGRGRFQRKVQLAQVCMSTGYEQVALPILEEISAEIDEHRLEEWEA
                     PEVVAHSLALLCRCIDKIAPDSPERRKIYARICRLDPMQALSCTG"
     gene            3204..3698
                     /gene="tssE"
                     /locus_tag="VFQ79_05840"
     CDS             3204..3698
                     /gene="tssE"
                     /locus_tag="VFQ79_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEU0139208.1"
                     /translation="MARGETGTAITQSVLDRLIDREPDSQAERPPTRAQSVRQLKAAV
                     RRDLEWLLNTRRNPEAAPDAASELARSLFVYGLPDITSFSLNSTKDQNRLAWLLENAI
                     KEFEPRLKAVRVRMETPGSGLRALRFQIEGMLAMDPVPERVTFDTLLELTSGSYRVKG
                     EPGA"
     gene            3691..5535
                     /gene="tssF"
                     /locus_tag="VFQ79_05845"
     CDS             3691..5535
                     /gene="tssF"
                     /locus_tag="VFQ79_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEU0139209.1"
                     /translation="MRDDLLDYYERELIFLRQMGAEFAQKYPKIASRLLLEPTQCDDP
                     HVERMLEAFAFLAGRVHLKIDDEFPEITDALLSVLYPHFVRPVPSMSIAEFHADPEQG
                     GLSTGLPVPRGTVLYSKPVDGFPCKFQTCYDTVLWPIAVADAQWKTPDRLQPALKYPG
                     AAACLRIELRCPPDIKFNLLEIPKLRIYLNGESNLISHLYELLNCNCLQILIRDVNPE
                     SRKKPVSLPPEALRPVGFAEDEGLMPGGRRSFIGYRLLQEYFSFPEKFFFLDITQLER
                     LASSQFEQGIEILFLISQFERPDRQQMLEQGVMQRTIRLGCSPIVNLFGQTAEPILLT
                     QMKPEYDVIPDVRRRTAMEVFSIDEVLSSDPQTGDTVAYDPFYSFRHSRARDRKQTFW
                     HAKRRASTRPNDAGTEVSLSLVDLSGRTSRPELDTITVRCTCTNRDLPFRLPFGNEAS
                     GDFEPEGLTPVRRVVALRKPTPAVRPPLGKGALWRLISHLSLNYLSLVDDGKDALQEI
                     LRLYEFTGSGFLEKQIAGIVGLSSKRHFARVISENGVAFVRGTRCEIEFDEEQFAGGG
                     VYLFASVLEHFLGLYVSINSFSQLVARTRQRRENLREWPPRAGQKILV"
     gene            5532..6533
                     /gene="tssG"
                     /locus_tag="VFQ79_05850"
     CDS             5532..6533
                     /gene="tssG"
                     /locus_tag="VFQ79_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEU0139210.1"
                     /translation="MRESPVEDLLRREPYRFEFFQAMRLLKRLQPGRAPVGRFTPPST
                     EAVRIGANPSMAFPASQIQALEWEGSGQPSMIVNFMGLTGPLGVLPLYYTELLLERKR
                     AGDTALLAFLDIFNHRITSLFYQAWEKYRFYVTYERGERDRFSRYLLDLIGLGSKGLQ
                     NRQAVLDDSLIYYSGLLALQPRSAAGLKQLIGDYFDVPVEVEQFAGGWYRLDRPTQTR
                     FQDRRTHSEQLGVGAVVGDEVWDPQSAVRLRLGPLPLARYLEFLPNGSAYDALRALTR
                     FYSNDGLDFEIQLVLERTEVPQCELGAGYEGGPLLGWVTWAASAPMERDPDETILKM"
     gene            6550..9213
                     /gene="tssH"
                     /locus_tag="VFQ79_05855"
     CDS             6550..9213
                     /gene="tssH"
                     /locus_tag="VFQ79_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEU0139211.1"
                     /translation="MSVNLKSLIGKLNDATRSALEGAAGLCLSRTHYDIELEHYLTKL
                     LDQTDGDAARIFRHFGVDTSRLNAELTRSLDKLKSGNARTPSLSPSVLKMMTDAWLLA
                     SVDFGWAEVRTGFTVLAMLTNEELVRIVRDVSKELQKIQPDALRNEFLSIVSGSHEEA
                     AGAAPADARSPEQVRAAGGKTPNLDQYTVDLTARARAGKIDPVLGRDFEIRQVIDILM
                     RRRQNNPILTGEAGVGKTAVVEGFAIRIAQGDVPPSLKNVILRTLDLALLQAGAGIKG
                     EFENRLKGLIEEVKASPTPIILFIDEAHTMIGAGGQAGQNDAANLLKPALARGELRTI
                     AATTWSEYKKFFEKDAALARRFQVVKVEEPTETQCMVMLRGITSNLEAHHGVRILDEG
                     LAGAVKFSHRYLAGRQLPDKAVSVLDTACARLALGQNSVPAAIEDAHRQIDDLIVQER
                     VLRREAAVGADHAERLEEIAKKQAETQERLANLEQRYEKEKDLVERVRKIRLELEGSP
                     AGAQSSDGSGTAAQPAKDPETLRSELALLNTELDALQGEAPLMRVCVDAHIVGEVISA
                     WTGIPVGKMMKDEISTVLELPKHLGSRVIGQGHALDAISQRIRTSRARLEDPNKPIGV
                     FLLVGPSGVGKTETALALADLLYGGERNLVTINMSEFQEAHTVSTLKGSPPGYVGYGE
                     GGVLTEAVRRRPYSVVLLDEVEKAHPDVLELFFQVFDKGMMEDGEGREIDFKNTIIIL
                     TSNAGTDTLMKLTADPETTPDSEGLVKALKPELNKIFKPAFLGRLVIIPYFPIRDEAM
                     KRIIVLKLGKIQRRIQENHKIILGYDDAVVDEVANRCTEVESGARNVDNILTNTLLPE
                     ISRQLLARMAAGEKLSSIRVEIGDGGSFMYS"
     gene            9265..11310
                     /gene="tssI"
                     /locus_tag="VFQ79_05860"
     CDS             9265..11310
                     /gene="tssI"
                     /locus_tag="VFQ79_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEU0139212.1"
                     /translation="MDLEFKQTHLPAQIQTPLPDSTLLLRSFTGREAISQLFSFELDL
                     IAQNHKTVEFEKLLGQKVTIGLEMPHDRGKRYWNGIVQRFSRGGRGVDFTSYRAALVP
                     SVWLLTKITRARIFQNMAVPDILRKVFTGFDVGYELTGRYEPHDFCVQYRESDFDFAS
                     RLMEEEGIFYFFKHSSGGHKMVVADTPQSHPDLQPERDVIFEELAGGARDEERVHAFE
                     KVQELRSGKYTLWDNTFLLPRRALDAEARTLESVQAGATTHKLNVGGNDKLELYDYPG
                     GYALRYDSGTTPSRVQRVFEDNRRTVGIRMQQEAAPALTIEGNGNCRHFVSGYKFSLQ
                     RHFNDDGQYVITAVDHMFSQANEFSVEDAGVDAGAIYENRFECIPAALPYRPQRLTPV
                     PSVHGTQTATVVGPAGEEIYTDEYARVKVQFHWDREGKNNADSSCWVRVATSWAGSNW
                     GAISLPRIGQEVVVDFLEGNPDRPIVIGSVYNAEQMPPYALPAKRTQSTIKSRSSKGG
                     APSNFNEIRFEDLKGSEQMFLHGEKDMDLRVKNDLREFVKRDRHLVVEGSQKELVKGN
                     KDVHAQGVHKQKVDGDVHLIAGGKRNEKVGTVLAVEAGTEIHLKAGSKIIIEGGQQVS
                     IKGAGGFIDIGPAGVTIQGTMVKINSGGAAGSGSGSNPQEPADPDVADDGTKVGKLN"
     gene            11320..12021
                     /locus_tag="VFQ79_05865"
     CDS             11320..12021
                     /locus_tag="VFQ79_05865"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEU0139213.1"
                     /translation="MPPAARITDNVTHPLPPVLTQGPGSPDVIIGGLPAWRGIPGAAV
                     AGLQAAKQASDVAIQTAEAATLAAAGTPGAPAAKAAEETTKATAAATMGSMISNLGAG
                     TDIHMCSTPLPVPPHGPGVVIDGSPTVLINNMPACRMGDTILEAVGPPNKIVRGEPTV
                     MIGNTGGGSPAAGGGGGGQGPGASGTGQTSSTGPQEGTGAQPVSTETGGVPRFGNALA
                     EAAETGDAFCEICLE"
     gene            12041..12886
                     /locus_tag="VFQ79_05870"
     CDS             12041..12886
                     /locus_tag="VFQ79_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HEU0139214.1"
                     /translation="MLATITIDAGPRAGESLKLRSGQSITIGRGAKAEFQITDDLFLS
                     SLHCLLEFRGPSLWIRDLGSSNGTLVNGRRVTECRLAGGDRIDAGRTKLVVSVSGDGA
                     PAGELKQAVLRVLRAQPGLFAILDAARDPMLLGKVRAFGEEFDSLFEGPHAEALVDFA
                     PYLCPLPPEGRLLAELVDSGWGNAWGVFLTCDETFAALRRHLRRFLTVKTEDGEKLYF
                     RFYDPRVLRIYLPTCNSAETRLFFGPIGSYLVEDRDPGRVIRFTPGADGVTREVIAAD
                     GDGGS"
     gene            12886..13284
                     /locus_tag="VFQ79_05875"
     CDS             12886..13284
                     /locus_tag="VFQ79_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139215.1"
                     /translation="MLVIRNAQMEAFKKHAEEQFIEDIIRHLNEHHRECIGELPDAEL
                     RTRVEKGLARARTYGIDDGRGLAAFVGLMFEIAPNFDEQPAVQTVLKDTSIPPGRRMD
                     ELIHRTSEDDWEQAEAMRDERVWTARGQGG"
     gene            13288..14451
                     /locus_tag="VFQ79_05880"
     CDS             13288..14451
                     /locus_tag="VFQ79_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU0139216.1"
                     /translation="MPKSKKAKLGKAKEKAKRTIAETGKVKKPEAKRQCRQKAVDYTA
                     EQVISESGAVVQDAESGLSIAKDLTTACSGQKKVQMPDWWLGTLGEQLVIGDLVGNGF
                     DGNKLTLKNVIQIKNESDNGIDLIVEAPDGSLYFFEVKSTETGKPGVLSKAQKDPEYF
                     VQACLADAASRSGRFARLSDQDVATAKRLYRSVTKGPPPEVHYIKADVMFPPKADPTD
                     PVDMTYSDWETGDVIDERQPVDDASTYSYMTAAGMGAMGEDAVIECLKKQGFDVLKSL
                     HNNSDNGIDIIARHPGSGRLCFLEVKTTTRDVAGKLQQAQRDAEGFAQSRLQRALAGT
                     HGWPPPDDDTKKVIRELLDDMTGDERYLKANVYMPQLGKKDSPVLVFEDWKKE"
     gene            14448..15332
                     /locus_tag="VFQ79_05885"
     CDS             14448..15332
                     /locus_tag="VFQ79_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139217.1"
                     /translation="MSVNPNWNAGELEQIIRTWLADESNHENLVVQPTAEIESYLSRH
                     GESDGGDAASELLWIFLWRLRQALIDLLDEKPDAPADMNRAHLYDFWRFRIRSAIFLQ
                     AAGRKRRAAFRFSEAGLLMAHALALGRFDEAATVGRILAAGIAEGMVSGEGVSVLGSF
                     MVQLYCRWRRQPLPQYRSEAADPGDYRRLLDTWSLQDEVAFAAAVEAACDFHTQLSGD
                     LDNEQYYEYTNEAWRLYPVEILAVLRLRQALGLNVPEISHPLLDSPLGVLYDPQSPPP
                     DPLLQRVIEKYRAELPAL"
     gene            15552..16331
                     /locus_tag="VFQ79_05890"
     CDS             15552..16331
                     /locus_tag="VFQ79_05890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0139218.1"
                     /translation="MRFDGKCVVVAGGGSGIGRAAAGLLALRGARVAVVDQNGKLAEE
                     TATAIRNEDGDAAAYTADISKFAEIDAAVSSEAERSGGIDALIVSAGIQRYGNALDTS
                     EEEWDRVLDVNLKGAWLAARAALPYLIVRQGAIVNVSSVQGMATQKNVLAYTVSKHGM
                     LGLTRSMAVDFAPNGVRVNAVCPGTVDTPMLEWSAGLAPDPAAVYEACAAMHPLGRIA
                     RPEEVAHVILFLAHSNSSFVTGSAWTVDGGLLTLLGGSPKG"
     gene            16350..17435
                     /locus_tag="VFQ79_05895"
     CDS             16350..17435
                     /locus_tag="VFQ79_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139219.1"
                     /translation="MTEIVRRREFIYSALGFSFAGAGWTQNRPERLDRNQLLVYRDAA
                     GQVKPVRTRGDWLKRRAEILRGMQEIMGPLPGREKRCPLDVRTEEEADAGTYVRRLVT
                     YAAEPGGRVPAYLLVPKALLDSGKRAPAVLCLHPTDNKIGHKVVVGLGGRPNRQYASE
                     LAERGYVTLAPPYPQLADYQPDLKALGYRSGTMKAIWDNMRGLDLLETLPFVRKGAFG
                     AIGHSLGGHNSVYTAVFDSRIQVIVSSCGLDSYLDYKDGDIRGWTSDRYMPGLLAYKD
                     RLQDIPFDFHEMIGALAPRMCFISAPLKDDNFKWQSVDRIAAAARPVYNLYGKPENLR
                     VEHPDCDHDFPDAMREIAYSLFDRHLR"
     gene            17448..19220
                     /locus_tag="VFQ79_05900"
     CDS             17448..19220
                     /locus_tag="VFQ79_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEU0139220.1"
                     /translation="MNRLALVLLFLVPLWAAETVKEHSVVVDASDATYTIDVAGTMDP
                     ENIAIELENLGDASVVSPRVTVNGKFDWYDVKSIAGEVTRGLTTDEEKAMALFQWVLY
                     KRYQRSPHDRSALHPVRAMNGYGYGICGHTSAWMKALWTVAGLRARVQELWGHTVSEV
                     FYDGAWHMLDANVKVFYLNRDNRTIASLAELEKDRTLIERTIHSHDPWVRQPDTREHN
                     EELVQYLVTAKDNFEEHAYDGEIEKNHTMAMSLKPGEKLVRWWKPVLGKFEGRDKRPE
                     VPSTYANGQLIWEPDLKRVDLKGYIHAMDNVATRGEGGRGLPVHIADLQDERHTRPSR
                     FTIPIHSSYPIIGGRLWCTLVKEGNSSLDQAAIFYGEPGWGGGNLYTFQWGKGSRDVE
                     FDLDPNIMRSGVVYNYTIGFNLRGNAKSDPPSQAGLERVRVVTDLQVSPHSLPALSLG
                     KNLVQFRDESAAGRRVRITHRWREVHDRRPPAAITVASTPRDGGEVQALAPVLKWNAP
                     TNNASGEIVDYQVMVSLRPDCRWPLSPTLHQNTGTAKTEWTVPQSFLNPGTTYYWKVR
                     ARNVHGDVGPWGSVFHFRTAAAAK"
     gene            19342..20973
                     /locus_tag="VFQ79_05905"
     CDS             19342..20973
                     /locus_tag="VFQ79_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139221.1"
                     /translation="MKKCATLLFVAVLFLQAQHNHHESTHQKPVELLEGLGDHTHPIA
                     TNSQMAQKFFDQGLALVFGFNHDEAARLFARAAELEPKSPMPHWGIALALGPNYNMPP
                     MPEREEKAWQAIEKAVELSKRAPENERAYVNALVKRYSKDPEEDRKQLAVHYKDAMKE
                     VMKRYPDDLDAATFYAEALMNLRPWQLWSKDGVPAEGTLEILEVLEGVLRRDPRHPGA
                     NHYYIHSVEASKNPERALPSAMRLGSLMPGAGHMVHMPSHIFLRVGDFEQSAMVNETA
                     SEVDRKYIERSGAKGIYPLMYYSHNLHFVSYARMLQGRFEQALDYSKRLRKNVDGAID
                     EMPMLGPYGAFEWLVLTRFGKWKEMLAEPEPSEKHPFMQAMYRYSRGLAFAGLGNVPE
                     AEAERERMGAFCTRIPESEMLMVNTMRSVLDIGMVELDARIARAKGDSAAEVAHFRRA
                     AELEDQLNYMEPPDWHYPVREALGGALLRQGKAAEAEAVFRRDLELHPRNGRSLFGLM
                     EAMKMQKKQVSVDWIQKEFQEAWRDSPMTLKVADL"
     gene            complement(21033..>21479)
                     /locus_tag="VFQ79_05910"
     CDS             complement(21033..>21479)
                     /locus_tag="VFQ79_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0139222.1"
                     /translation="ILMSPRMWRKLVAPHHERLNRRVHELGTRVMYHSCGAVRPFIPA
                     LIDIGTDVLDVLQFSAEGMNPQEIKSTFGDRLCFHGGMDVQATLPRCTPGEVHQTARE
                     HIAVLGAGGGYILSPTHNIQADTPPANILAMYEAAGSLSPELATQK"
BASE COUNT         4539 a         5974 c         6906 g         4060 t
ORIGIN      
        1 ctctgcgcct ggtcgcggag ctgccggcct cggctcgcaa gtaagtaaga cggtacgctt
       61 tctgatgatg gaacggagcg gacggggtag gtctgtcttc gaaacggcaa ctttgtatcg
      121 gaaggagaag aatcgaaatg gctgttgatc tttatttgaa aattgatgga atcgaaggcg
      181 aaagcatgga ttccaaacac gggaaagaaa tcgaagtaga atccttcagt tggggtgaaa
      241 cgcaatccgg cacctcaacg cacggggccg gcatgggggc gggaaaagtc aacatgcagg
      301 actttcactt cgtgatgcgc gtgaacaagt ccagtccgaa gttgatgctt gcctgcgcca
      361 gcggcgagca catcaagtcg gcgatcctca cctgccgcaa ggcgggcaag gaccagcagg
      421 agtttctgaa gatcacgttc tcggatctgc tggtgtcgtc ttaccagacc ggcgccgcct
      481 ccggggtcga ttcgataccg atggaccaga tttccctgaa ttattcgaaa atcgagtacg
      541 aatacaagga gcagaaggcg gacggcacgt tggccggagc catcaagacc ggttgggacc
      601 tgaagctgaa caagaaggtc tagcgaatcg agcccgacgc tatgacagcc agagaactgt
      661 ttcaggcggg ccggctcaac gaatccgtgc aggcgctggg tgcggaactt cggaaagatc
      721 cgaccgacac ccggcgccgc acctttttgt tcgagctgct ttgcttcgcc ggcgagtatg
      781 aacgcgccga aaagcaattg gatattctgg ccagcgaaaa aaaagaatcg gacttgggtg
      841 ccctgctgta tagggctgcc atccatgccg aacggacgcg gaacgaaatg ttctcgaagc
      901 gggatttccc ttccggtccg gcatcggaac aggtagacgg tactttcgaa gggcagccct
      961 tctccaccat tgaggatggc gacccgcgaa ttggaccgag gctcgaagtg tttgccgccg
     1021 gggcttacct gtggattccg ttcgagcatc tcgcctccat cgaaatcccg gagcccaaac
     1081 gccttcggga tctgatctgg acacctgcac tggttcgcac cggacctgcc tttaagggca
     1141 aggaactggg agaggttttg ctgccggtgc tgactcctct ttcgagcaga caccccgatg
     1201 acaacgttcg cctgggccga tctacgattt gggaggagac ggaagacggc cgggtggtgc
     1261 cggcggggca aaaaatgata gtagtggacg gggaggaagt gccgatcctc gaaattcgca
     1321 gcattcattt cgtcgcaacg caagcggccg cttagaaaat gcctttgagg gacgatctgc
     1381 tgacgccgat tccgggcgct aatccgggcg gcgagaacct acgctattcg accatcgtcg
     1441 acaagatcaa agaagcgagg cgcgaagagg acgacgcgcc gcagggcgaa tggcagcgcg
     1501 agcgcaaggt agccgattac gttaccgtca tcaaactggc cggcgatgcc attgcggcca
     1561 aaagcaaaga tctgcaactg gccacatggt taacggaagc gcttctgaag cgcgagggta
     1621 ttgccgggct tcgttcgggc cttcaactca ttaagagctt gctggagaat ttctgggacc
     1681 acctgtatcc ggaactggag gacggcgatc cggaaatgcg cgccgtaccg ctcgaatgga
     1741 ttgggacgaa gctggatgat gcggtgcgca aggcgcctct gaccaaaagc gggtacgact
     1801 ggtacagcta taaggactcc cgcgccgtcg gctatgagga ggcgtgcgag agcgattccg
     1861 ccaagttgga agcccgcgcc ttgaagatcg ccgatggcaa gctgacgccg gaggagttcg
     1921 acaaggcatt caccgccaca ccgaaagatt tctacgcgaa gaccaccgaa gaccttgaag
     1981 ctactctcgt tcttctggaa gagctgtctg cgctgggcga agagaaattt ggcgatgtcg
     2041 cgccgggtta cggcagactg aagaccgcta tcgaggaagt gcagaatacg gtccgcatac
     2101 tgcttcagaa aaagcgcgag gtggagccgg acgacgagcc cgccgtggaa gagcagccag
     2161 aggagccttc tttcatcgaa gaaacggtaa ccgccggcga gagcggctcg gagaccgcgg
     2221 cggcgcctgt acgcgctccc gtccggacgg caaaaaaggt gacgagtatc gaaccggtgg
     2281 accgcggcga tgccgtggat cgcgtgacgg ccgccgcacg atttctgcgg caacaggacg
     2341 tatacgatcc ggttccctac ctgatgatcc ggggactgcg ctggggcgaa ctgcggactt
     2401 cggagaccat cgacgccacg ttgctggttc ccccggcgac ggaaatgcgg cagcgcatca
     2461 agcaggcttc gctcgaagga aattgggaag cggtgctcaa cgccgccgaa gaggcgatgg
     2521 ccatgccctg tggccggggc tggctggatc tgcaacgata tgtcgtgcgt gcgtgccagg
     2581 aattggggag ttattacgat cgcatcgcgg cggcggtgaa gtcgtcgctt cgggagctgc
     2641 tcgccgatta tccccacctg ccggaaatga cgttgatgga tgacacgccc gtggcaaatc
     2701 cggagacgca gcagtggctc cgcggagaga tcgtacccgt gtcccccagt cctttctcca
     2761 ccgcaataac ggccacccct atccggtccg gcccggagga ttccgcggag gagaatgcgg
     2821 aaccgaagcc gcccgatgcg tttgagatcg caatgcaggc cgttcacaac ggacacgtgc
     2881 aggaggcgat tgagatcctg atgcgtgaag cggcgcagga gcagtcgggc cgcggccgtt
     2941 tccagcgtaa ggtacagctt gcgcaggttt gtatgagcac cggctacgag caggtggcgc
     3001 taccgattct ggaggagatt tcggccgaaa tagacgagca ccggctggag gagtgggagg
     3061 cgccggaggt ggtggctcac tctctcgcac tgctctgccg ctgcatcgac aagatcgcgc
     3121 cggattcacc ggagcggcgc aagatttatg cgcgaatctg ccgcctggat ccgatgcagg
     3181 ccctttcgtg cacggggtaa gcgatggcga gaggggaaac cggaactgca atcacacaat
     3241 cggtactgga caggttgatc gatcgggaac ctgattcaca ggcggagcgg ccgcctacgc
     3301 gggcgcagtc cgtacggcag ctcaaagcgg cggtccggcg ggatctcgag tggctgctga
     3361 acacgcgcag gaatccggag gcggcgccgg atgccgcgag cgaacttgcg cgctctctat
     3421 tcgtctacgg cttgcccgat atcacatcct tcagcctgaa ctcgaccaag gaccagaacc
     3481 ggctggcctg gctgctggag aacgccatta aggagttcga gccgcgcctg aaagcggtaa
     3541 gagtgcgaat ggaaacgccc ggctccgggt tgcgggcgct tcgctttcag attgagggaa
     3601 tgctggcgat ggatccggtg ccggagcgtg ttacgttcga cacgctgctg gagctcacca
     3661 gcggctcgta ccgcgtaaag ggagagcccg gtgcgtgatg acctgctcga ttattacgag
     3721 cgtgaactga tttttttgcg ccagatgggt gcggagttcg cacagaagta tccgaagatc
     3781 gcatcgcggc tgctgctcga acctacacag tgcgacgatc cgcacgtcga gcgaatgctc
     3841 gaagcattcg cattccttgc cggccgcgtt catctgaaaa tcgatgacga gtttcccgag
     3901 atcaccgacg cgctgctcag tgtgttgtac ccgcatttcg tgcggccggt gccatcgatg
     3961 tctatcgccg agtttcacgc ggaccccgag caaggcggct tatctaccgg gctgccggtt
     4021 ccacgcggaa cggtcctcta ttcgaaaccg gtggacgggt ttccgtgtaa gtttcaaacc
     4081 tgctatgaca cggtgctatg gcccattgcc gttgccgatg cgcaatggaa gacgccggac
     4141 cgtctgcaac cggccttgaa atatcccggt gcagccgcct gtctgcgtat cgagctgcga
     4201 tgccctcccg acatcaagtt caacctgctg gagattccca agctccggat ttacctgaac
     4261 ggcgaaagca atctcatcag ccacctctat gaactgttga actgcaattg cctccagatt
     4321 ctcatccggg acgtgaaccc cgagagccgc aagaaaccgg tttcgctacc gcctgaagcg
     4381 ctacgtcctg tcgggtttgc cgaagacgaa gggctgatgc ccggcggaag acgatctttc
     4441 atcggctacc ggctgttgca ggagtatttc agttttcccg agaagttctt tttcctcgat
     4501 atcactcaac tggagcgtct ggcgtcttcg cagttcgaac agggaatcga gatccttttt
     4561 ctgatatcgc agtttgagcg tcccgacagg cagcagatgc tggaacaggg cgtcatgcag
     4621 aggaccattc gtttgggatg cagcccgatc gtcaacctgt tcggacaaac agccgagccg
     4681 attctgctga cccagatgaa gccggaatac gatgtgattc ccgacgtgcg ccgccggacc
     4741 gccatggagg ttttctccat tgatgaggtg ctcagctccg acccgcagac cggcgatacc
     4801 gtcgcctacg atccgttcta ttcgttccgg cactcccggg cgcgcgacag gaaacagacc
     4861 ttctggcacg ccaagcggcg cgcttctacg cgtccgaatg acgctggtac cgaagtgagc
     4921 ctgtcgctcg tggatctttc cgggcggacc tcgcgcccgg agctcgatac cattacggtg
     4981 cgatgtacgt gtacaaacag ggacctgcca ttccggttgc ccttcgggaa tgaggcatcg
     5041 ggggacttcg agccggaggg gctgacgccg gtccgccgcg tggtggcact gcggaaaccg
     5101 accccggcag tgcggccgcc gctcggcaag ggggcgctgt ggaggctgat ttcgcatctg
     5161 tctctgaact acctttccct tgtggacgac gggaaagacg cgctgcagga gattctgcgg
     5221 ctgtatgagt ttacaggatc cgggtttctc gaaaagcaaa tcgccggcat cgtgggcctg
     5281 tcgagcaaac ggcacttcgc tcgcgtcatt tcggaaaacg gcgtggcctt tgtgcgcggt
     5341 acgcgatgcg aaatcgaatt cgatgaagag cagttcgccg gcggcggcgt atacctgttt
     5401 gcgagcgtgt tggaacattt tctggggctg tatgtttcca tcaacagctt cagccagttg
     5461 gttgcccgga ccaggcagcg aagggagaat ctgagggagt ggccaccgcg ggccggacag
     5521 aaaattctcg tttgagggaa tcgccggtag aagacctgct tcggcgggag ccctaccgtt
     5581 tcgaattctt tcaggcaatg cggctgttga agaggcttca gcccggccgt gcgccggtgg
     5641 ggcgttttac gccgccttcg accgaagcgg tgcgcattgg cgcgaacccg tcgatggcgt
     5701 ttcccgccag ccagattcag gcgcttgagt gggaaggtag cggacagccg agcatgatcg
     5761 tcaatttcat ggggcttacc ggtccattag gagtgctgcc gctctactat acggaactgc
     5821 tgctcgaacg gaaacgtgcg ggagacaccg ccctgctcgc gttcctcgac attttcaacc
     5881 accgcattac gtcgctgttc taccaggcgt gggagaagta ccgtttttac gttacctatg
     5941 aacgcggtga gcgcgaccgg ttttcgcggt atctgctcga cctgattggc ctgggaagta
     6001 aaggactgca aaaccggcag gcggttctgg acgattcgct gatttactat tccgggctgc
     6061 tcgcactcca gccgcgttcg gccgcaggcc tcaagcaatt gatcggggac tacttcgatg
     6121 tgcccgtaga agtggagcag tttgccggcg gatggtatcg gctggaccgt cccacgcaga
     6181 cgcgattcca ggaccgccgg acgcattccg agcagttggg cgtgggcgcc gtcgtgggcg
     6241 atgaagtctg ggacccgcaa tcggccgtgc ggctccgcct gggcccgctg ccgcttgcgc
     6301 gctacctgga gtttctgccg aacgggagtg cgtacgacgc gttgcgcgcc ctgacgcgat
     6361 tttactcgaa cgatggtttg gacttcgaga ttcaactggt tctcgaacgg acggaggtgc
     6421 cgcagtgcga actcggcgcc ggctatgaag gtgggcccct gttggggtgg gtcacctggg
     6481 cagcgtccgc gcccatggag cgcgaccccg atgaaacaat tctgaagatg tgagcggaag
     6541 gagatattga tgagcgtcaa tctgaaatca ttgatcggga agttgaacga tgccacccga
     6601 agcgcgctgg agggggcggc cgggctgtgc ctttcgcgca cacactatga catcgagctg
     6661 gaacattacc tgacgaaatt gctggatcag acggatggtg acgcggcgcg cattttccgg
     6721 catttcggcg tcgacacctc gcgcttgaat gcggagctga cacgttcgct ggataaattg
     6781 aaatccggga acgcgcgaac accgtccctg agtccttcgg tgctcaagat gatgacggat
     6841 gcctggctgc tggcgtcggt ggatttcggc tgggccgagg ttcgcacggg ctttacggtg
     6901 ctggcgatgt tgacgaatga ggaactggtc cgtatcgttc gggacgtcag taaggagctg
     6961 caaaagatcc agcccgatgc gctgcgcaac gaattcctga gcatcgtgtc cggctcgcat
     7021 gaagaggcgg ccggtgcggc tccggcggac gcgagaagcc cggaacaggt gcgcgcggcc
     7081 ggcggtaaga ctccaaacct cgatcagtat acggtcgatt tgaccgcccg cgcccgggcc
     7141 gggaagatcg atcccgtttt aggcagggat ttcgaaatcc gccaggtgat cgacatcctg
     7201 atgcggcgcc gtcagaataa ccccattttg acgggagagg cgggcgtagg aaaaacggcc
     7261 gtggtggagg gattcgctat tcgcattgcg cagggggatg tgccgccttc tctcaagaac
     7321 gtcattctgc gcaccctcga cctggcgctg ctgcaagccg gcgcgggcat caaaggcgaa
     7381 ttcgagaacc ggctgaaagg attgattgag gaagtcaagg cgtcccccac acccatcatt
     7441 ctttttatcg atgaagcgca cacgatgatc ggggcgggcg ggcaggccgg tcagaacgat
     7501 gcggccaatc ttttgaaacc ggcccttgcg cgaggcgagc tgcggaccat tgcagccacc
     7561 acgtggtccg agtacaaaaa gtttttcgag aaagacgcgg ccctggcccg ccggttccag
     7621 gtcgtgaagg tggaagaacc aacggagacg cagtgtatgg tgatgctgcg cggcattact
     7681 tcaaacctcg aagcgcatca cggtgtccgg attctggacg aagggctcgc gggtgcagtg
     7741 aagttctcac accggtatct tgccgggcgt cagttgcccg acaaagccgt tagcgtgctc
     7801 gataccgcgt gcgcccggct ggcgctgggc cagaattccg tcccggcggc gatcgaggat
     7861 gcacaccggc agatcgacga cctgatcgta caggagcgag tgttgcgacg tgaggctgcc
     7921 gtgggagctg accatgcgga gcgcctcgaa gagatcgcga agaagcaggc cgaaactcaa
     7981 gagcgcctgg cgaatctcga acagcgctat gaaaaggaga aggacctggt ggagcgtgtt
     8041 cgcaaaatca ggttggagct ggaaggatcg cccgccgggg cgcaatcgtc cgacggcagt
     8101 ggaacggcag cgcagccggc gaaagatccg gagacgctgc ggtctgagct ggccctgctc
     8161 aacacggaac tggatgcgct gcaaggcgag gcgccgctga tgcgggtttg cgtggacgca
     8221 cacatcgtgg gcgaagtgat ctcggcgtgg accggcattc cggtcgggaa gatgatgaaa
     8281 gacgaaatct ctaccgtgct ggaactgcca aagcatctcg gctcgcgggt gattggacag
     8341 ggccacgcgc tcgatgccat cagccagcgc atacggactt cgcgtgcgcg cctggaagat
     8401 cccaataaac cgatcggtgt tttcctcctg gtaggtccca gcggtgtcgg taaaacggaa
     8461 acggcgctgg ctctggccga cctgttgtac ggaggggaaa gaaaccttgt caccatcaac
     8521 atgtcggagt tccaggaggc gcacaccgta tccacgctga aaggttcgcc tcccggatat
     8581 gtcggctacg gagagggcgg cgtactgacg gaagcggtcc ggaggcgtcc ctattccgtg
     8641 gtgctgctgg atgaagtgga aaaggcgcac ccggacgtgc tggagctttt cttccaggtc
     8701 ttcgacaagg gaatgatgga agatggcgaa ggccgcgaga tcgatttcaa aaatacgatc
     8761 atcattctga cgtcgaacgc cggcaccgat acgttgatga agctgacggc ggaccctgaa
     8821 acgacgcccg attcggaagg tctggtaaag gcgctcaaac cggagcttaa caagatcttc
     8881 aagcccgcat ttctcggccg gcttgtcatc atcccgtact tcccgattcg cgacgaggcg
     8941 atgaagcgga tcatcgttct gaaacttgga aagattcagc gccgcattca ggaaaaccac
     9001 aagatcattc tcggctacga tgacgccgtc gttgacgaag tggcgaaccg gtgcactgag
     9061 gtggagagcg gcgcccgtaa cgtcgacaac attctcacca atacgctttt gcctgaaatc
     9121 tcgagacaat tgctggcgcg gatggcagcg ggagaaaaac tcagttcgat ccgtgtggag
     9181 attggggatg gcggctcctt catgtactcc tgacacgcgt tcaaccgcgg ggcgctttgc
     9241 gccccggtag ttcggaggta ggaattggac cttgaattca agcagactca ccttccagct
     9301 caaatccaga cgccgcttcc ggacagtacg ctgctgctcc ggagttttac aggtagagaa
     9361 gccatctcgc agttgttcag cttcgaactc gatctcatcg cccagaatca caagacagtg
     9421 gaattcgaga agcttctggg gcagaaggtc accatagggc tggagatgcc gcacgaccgg
     9481 gggaagcgtt attggaacgg tatcgtccag cgcttttcac ggggagggcg gggtgtcgac
     9541 ttcaccagct accgggccgc tctggttccg tcggtgtggc tgctgaccaa gatcacgcgc
     9601 gcgcgtatct ttcagaacat ggcggtgccg gacatcctca ggaaggtttt caccggtttc
     9661 gacgtgggct atgaactcac cggacgctat gagccgcacg acttttgtgt gcaataccgg
     9721 gagtccgatt tcgacttcgc gagcaggctc atggaagagg aaggaatttt ctatttcttc
     9781 aaacactcct ccggggggca caaaatggtg gtcgccgata cccctcaatc ccatccggat
     9841 cttcagccgg agcgggacgt catcttcgaa gaactggcgg gcggcgcccg ggatgaagag
     9901 cgcgtgcacg cgttcgagaa agtgcaggag ttgcgatccg gaaaatatac gctgtgggac
     9961 aacacgttcc tgctgccccg gcgggctctg gatgccgaag ccagaacgct tgaaagcgtg
    10021 caagccggtg caaccaccca caagctgaat gtcgggggca acgacaagct cgagttgtac
    10081 gattacccgg gtggatatgc attacgctac gactcgggaa caacgccgtc ccgggtgcaa
    10141 cgtgtcttcg aagacaaccg ccggaccgtg ggcatccgca tgcaacagga ggcggcgccg
    10201 gcgctgacca tagagggcaa cggaaactgc cggcattttg tctccgggta caagttctcg
    10261 ctccagcggc acttcaacga cgatgggcag tacgtcatca cggcagtgga ccacatgttc
    10321 agccaggcga acgaattttc ggtggaagac gcgggcgtgg acgccggcgc catctacgag
    10381 aatcgtttcg aatgcatacc ggccgcgctg ccctaccgtc cgcagcgcct gacgccggtt
    10441 ccctcggtgc acggtaccca gaccgcaacc gtggtgggtc ctgccggaga ggagatctac
    10501 acggacgaat acgcgcgtgt gaaagtgcag tttcattggg atcgcgaagg gaagaacaac
    10561 gcggacagct cctgctgggt tcgtgtggcc accagttggg cggggagcaa ctggggcgcg
    10621 atctcgcttc cgagaatcgg gcaggaggta gtggtcgact ttctggaagg aaatcccgac
    10681 cggcccatcg tcatcggcag cgtgtacaac gcggaacaga tgccgcctta tgcgctgccc
    10741 gccaagcgca cccagagcac gatcaagagc cgcagctcga agggcggggc accctccaat
    10801 ttcaatgaga ttcgcttcga agacctgaaa ggctccgaac agatgttcct tcacggcgaa
    10861 aaggacatgg atctgcgcgt gaagaacgat cttcgcgagt tcgtcaaaag ggaccggcac
    10921 ttggttgtcg aaggcagcca gaaggagttg gtcaaaggaa acaaggacgt ccacgcccag
    10981 ggtgttcata agcagaaggt ggacggagat gtgcacctga tagccggagg caagcgcaac
    11041 gagaaagtgg gcacggtgct ggccgtcgaa gcgggcacgg aaatccacct gaaggccggc
    11101 agtaagatca tcatcgaagg cgggcagcag gtcagcatca aaggcgcggg gggattcatc
    11161 gacatcggtc ccgcgggcgt caccatccag ggcacgatgg tgaagatcaa cagtggcggc
    11221 gcggcgggat cgggtagcgg atcgaatccc caggaaccgg cggaccccga tgtcgccgac
    11281 gacggcacga aggtaggcaa gctgaattag gagaaatgga tgccaccggc agcgcgcatc
    11341 acggataacg tcacccatcc gctccccccg gtgctgaccc aggggcccgg cagtccggac
    11401 gtaattatag gcgggctgcc cgcgtggcgt ggcattcccg gcgcggccgt tgcgggactt
    11461 caagcggcca agcaggcgtc ggatgttgcg attcagactg cggaagcggc tacgctcgcg
    11521 gcggcgggca ccccgggtgc gcctgccgcg aaggcggctg aagagaccac caaggcgacg
    11581 gcagccgcca cgatgggaag catgatttca aacctcggcg cgggcaccga catacatatg
    11641 tgcagcacgc cgctgcccgt gccgcctcac ggtccgggcg tggtcatcga cggatctcct
    11701 acggtactca tcaataacat gccggcgtgc cgcatgggcg acacgatcct cgaagctgtc
    11761 ggaccaccca ataagatcgt aaggggcgag cccaccgtca tgattggcaa cacaggcggc
    11821 ggaagcccgg ccgcaggagg tggcggcggt ggacaaggtc cgggtgcatc cggcaccggc
    11881 cagacgtcca gcacgggccc acaggaaggc accggagcgc agccggtcag caccgaaacg
    11941 ggcggcgttc cccgtttcgg aaacgcgctc gctgaagccg cggagaccgg agacgccttc
    12001 tgcgaaatct gtctcgaata ggatcccaag ctgagttgag atgctcgcga caataacgat
    12061 cgatgccggg ccgcgcgccg gtgagagcct gaagttgcgg tcaggccaga gtatcacgat
    12121 aggcagaggt gcaaaagccg agtttcagat tacggacgat ttgtttctat cgtcgctcca
    12181 ctgcctgctg gaatttcgcg ggccctcgct gtggatcagg gatcttggca gctcgaacgg
    12241 tacgctcgta aacggccggc gcgtgacgga gtgccgtctg gccggcggag accggatcga
    12301 cgcggggcga accaagctgg tggtctccgt gtccggcgat ggagcgcccg cgggtgaact
    12361 caagcaagcc gtgctgcgtg tcctgagagc gcagccagga ctcttcgcta ttctggatgc
    12421 ggcgcgggac ccgatgctgc ttgggaaagt gcgcgcgttt ggagaggagt tcgactcgct
    12481 gttcgaaggc ccgcatgccg aagcgctggt ggattttgcc ccctacttat gtccccttcc
    12541 gcctgaaggc cggctgcttg ccgagttggt agatagcggc tggggcaatg cgtggggtgt
    12601 cttccttact tgcgacgaga cgtttgccgc gctgcggcgg cacttgcggc gcttcctgac
    12661 ggtgaagacc gaagatggcg aaaagctata tttccgtttc tacgatcccc gcgtgttgcg
    12721 aatctatctg ccgacctgca attccgccga aacccggctg ttttttggtc ccatcggctc
    12781 ctacctggtc gaagaccgcg accccggacg ggtcatccgg tttacgccgg gagcggatgg
    12841 cgtcacccgt gaagtgatcg cggcggacgg cgatgggggc tcctgatgct ggtcattcga
    12901 aacgctcaga tggaagcttt caagaagcat gccgaggagc aatttatcga agacataatc
    12961 cgccatctga acgaacacca ccgcgaatgc atcggcgaac tgccggatgc ggaattgcgt
    13021 actcgcgtcg aaaaggggct cgcgcgcgcg agaacatatg gaatcgacga cggccggggc
    13081 ctggcggcct tcgtgggact gatgtttgag atcgctccga acttcgacga gcagccggcg
    13141 gtccaaactg ttttgaagga cacgtcaata cctcccggac gcaggatgga cgaactgatc
    13201 caccgaacct ccgaggacga ttgggagcag gcggaagcga tgcgggacga acgggtgtgg
    13261 acggcccgcg ggcagggggg ctgagtcgtg ccgaagagta aaaaggcgaa gctggggaaa
    13321 gcaaaggaga aggctaagcg cacgatcgcc gaaacgggca aagtaaaaaa accagaagcg
    13381 aagcggcaat gccggcagaa agcggtcgac tatacggccg agcaggtcat ttccgagagt
    13441 ggcgccgtgg tgcaggacgc cgagagcggg ctctccatcg ccaaagacct caccacagcc
    13501 tgtagcggcc agaagaaggt gcagatgccg gattggtggc tgggaacgct cggcgaacaa
    13561 ctggtgatcg gcgatctggt gggaaatggt ttcgacggca acaagctcac gctcaagaac
    13621 gtgatccaga tcaagaacga gagcgacaac ggcatcgacc tcatcgtgga ggcgcccgac
    13681 ggcagcctct atttcttcga ggtgaaatcg acggagaccg ggaaaccggg ggtgctctcg
    13741 aaagcccaga aagacccgga atatttcgta caagcatgcc ttgccgatgc ggcgtcacgc
    13801 agcggcaggt ttgcccgctt gtcggaccag gatgtcgcga cggccaagcg gctataccgc
    13861 tccgtgacga agggtccccc gcctgaagtg cattacatca aagcggacgt aatgtttcct
    13921 ccaaaggcgg accccaccga tccggtggac atgacgtaca gcgactggga gacgggagat
    13981 gtcattgacg agcggcagcc ggtagacgat gcaagcacct atagctacat gaccgcggcc
    14041 gggatgggcg caatgggaga agacgccgtc atcgaatgtc tgaagaagca gggctttgat
    14101 gtgttgaagt ccctgcacaa caactcggat aacggcatcg atatcattgc acggcatcct
    14161 ggttccggcc ggctctgctt cctggaagta aagacgacaa cccgggatgt tgccggcaaa
    14221 ctacagcagg cgcagcgcga cgcggaaggt tttgcgcagt cgcgcctgca acgcgccctt
    14281 gcgggaacgc acggttggcc cccgcccgac gacgatacaa agaaagtcat tcgtgagctg
    14341 ctggatgaca tgacggggga cgagcgatac ctgaaagcta acgtttacat gccccagtta
    14401 ggtaagaagg attcgcccgt tctggtattc gaagattgga aaaaagaatg agtgtgaacc
    14461 cgaactggaa tgccggggag ctcgaacaga ttatccgcac atggttagcg gatgagagca
    14521 accatgaaaa cctggtggtg cagcctaccg ccgagatcga aagctatctg agccggcacg
    14581 gcgaaagcga tggaggggac gctgcgtccg agttgttgtg gatcttcctt tggcggctgc
    14641 gccaggcgct gatcgatctt cttgacgaga agccggacgc gcccgctgat atgaaccgcg
    14701 cgcacctgta cgacttctgg cggttccgca ttcgaagtgc gatcttcctt caggcggccg
    14761 gcaggaagcg tcgtgccgcg ttccgattca gcgaggccgg ccttctgatg gcgcacgcgc
    14821 tcgcgctcgg gcgattcgac gaagctgcaa ccgtagggcg gatactggcg gccggcattg
    14881 cggagggcat ggtctcggga gaaggagtga gcgtcctggg gtctttcatg gtgcagcttt
    14941 attgccgttg gcggcggcag ccgttacccc aatacaggag cgaggccgcc gatcccggcg
    15001 attaccggcg gctgcttgac acctggtccc ttcaggatga agttgctttc gcagcggcgg
    15061 tggaagccgc ctgtgatttc cacacccagt tgagcggtga cctggataac gagcagtact
    15121 acgagtacac gaatgaagcg tggcgcctct atccggtcga gattctggct gtgctacggt
    15181 tgcgccaggc attgggtctg aatgtgccgg agatctcgca tccgcttctc gattcccctc
    15241 ttggcgtcct gtacgaccca cagtcgcctc cacccgatcc tcttctccaa cgtgtgatcg
    15301 aaaagtaccg tgcagaactg ccggcgctgt agaccgctag aactcgtgcc actataacta
    15361 aacacgacgt cctaaatgtt tgcgcgacga tttgattggc cacggatgaa cacggattca
    15421 cacggataag aaaaacgcaa ggcttgattc gtgtgcatcc gtgtccatcc gtggctgatt
    15481 ttgcttttct agattgctgg gatgcgcaat tatttatgac gttctgttta tatttgacat
    15541 aggagacgcg aatgcgattt gacggcaaat gcgtagtagt ggcaggaggt ggctcgggta
    15601 ttggcagggc ggcggcggga ttgctggcct tgcgcggcgc gcgtgtggca gtcgtcgacc
    15661 agaacggaaa actggctgaa gaaaccgcta cggcgattcg aaacgaagac ggcgatgccg
    15721 cggcctatac ggccgacatt tcgaagttcg cggagatcga cgctgccgtt tcttctgagg
    15781 cggagcgatc cggcggtatc gacgcgctca ttgtatccgc cggcatccag cgctacggaa
    15841 atgctctcga tacaagtgag gaagaatggg accgggtcct ggacgtgaat ctcaagggtg
    15901 cgtggctggc cgcgcgcgct gccctgccgt acctgatcgt ccggcagggg gcaatcgtca
    15961 acgtctcgtc cgtgcaggga atggccacgc agaaaaacgt gttggcctat acggtaagca
    16021 agcacggcat gttgggcttg acgcgctcga tggccgtgga cttcgcgccg aatggagtgc
    16081 gcgtgaatgc ggtctgcccc ggcacggtag acacgccgat gctcgagtgg tccgcggggc
    16141 ttgcgccgga tcccgccgcc gtgtatgaag cgtgcgctgc gatgcatccg ttgggccgga
    16201 tcgcgcggcc ggaggaggtc gcacacgtga ttctctttct cgcccattcg aattcgagtt
    16261 tcgttaccgg atcggcctgg acggtggatg gcggcctgtt gacgctgctc ggcggatcac
    16321 cgaaagggtg atagcgtaat aaagttacta tgaccgaaat tgtccgccgc cgcgaattca
    16381 tatactcggc gcttggtttc agcttcgcgg gagcgggctg gacccaaaac cgcccggagc
    16441 gcctggatcg caaccagctt cttgtctacc gcgacgccgc cgggcaagtg aagccagtac
    16501 ggacgcgcgg cgactggctg aagaggcggg ctgaaatcct gcgtggcatg caggaaataa
    16561 tgggaccgct gcccggacgc gaaaagcgtt gtccgctcga tgtacgaacg gaagaggagg
    16621 ccgatgccgg aacctatgtg cggcgcctgg tcacctacgc cgcggagcct ggcggccgcg
    16681 tgccggctta tttgctggtc cccaaggcgt tgctggattc gggcaagcgg gctccggcgg
    16741 tgttgtgcct gcatcccacg gataacaaaa tcggccacaa ggtcgtggtg ggactgggcg
    16801 gccgtccgaa ccggcagtat gcgtcggagc ttgcggagcg cggttatgtc accctggcgc
    16861 caccgtatcc gcaactggcc gattatcagc ccgacctgaa ggcgctcggc tatcggagcg
    16921 gcacgatgaa ggcgatttgg gacaacatgc gcgggctgga tttgttagag acgctgccat
    16981 ttgtccgcaa aggcgctttc ggcgcgatcg gacattcgct gggtggacac aattcggtat
    17041 acacggccgt gttcgattcc cgaattcagg tgatcgtctc gagctgcggc ctcgattcat
    17101 atctggatta caaagacggc gatattcgcg gctggaccag cgaccgttac atgcccgggc
    17161 tgctcgctta caaagaccgg ctccaggaca ttccgttcga ctttcacgag atgatcggag
    17221 cgctggcgcc gcgcatgtgc ttcatcagcg cgccgctcaa ggacgacaac ttcaagtggc
    17281 agagtgtgga ccggattgcc gccgcggcgc ggcctgttta taatttgtac ggcaaaccgg
    17341 aaaatctgcg agtagaacat cccgactgcg accacgattt tccggatgcc atgcgcgaga
    17401 tcgcgtattc acttttcgat cggcacctgc gttagcgaag gagcggcatg aatcgccttg
    17461 cacttgtctt acttttcctc gtaccgctat gggcggcgga gactgttaag gagcactctg
    17521 tcgtcgtcga tgcttcagac gccacttaca cgatcgatgt ggccggcacg atggatccgg
    17581 agaacattgc catagaactg gagaatctgg gcgacgcatc ggtggtttct ccccgcgtta
    17641 ccgtgaacgg aaaattcgac tggtacgacg tgaaatcgat tgccggcgag gtaacgcgcg
    17701 gattgaccac tgacgaggag aaggcgatgg cgctcttcca gtgggtgctg tataaacgct
    17761 accagcgatc gccccacgac cgctccgcgc tgcatcccgt gcgcgcgatg aacggctatg
    17821 gatacggcat ctgcgggcac acctcggcgt ggatgaaggc gctctggacc gtggcaggat
    17881 tgagggcgcg tgtgcaggaa ttatggggac acacggtttc cgaagtgttt tacgacggcg
    17941 cctggcacat gctggacgcc aacgtgaaag tgttctacct gaaccgggac aaccgcacca
    18001 tcgcgagctt ggcggaactc gaaaaagaca gaacgctcat cgagcgcacc attcattcgc
    18061 acgatccatg ggtccgccag ccggacacac gcgagcacaa tgaagaactg gtgcaatacc
    18121 tggtgacggc gaaggacaac ttcgaagagc atgcttacga cggcgagatc gaaaagaacc
    18181 acaccatggc gatgtcgctg aaacccggtg aaaagctggt ccgatggtgg aagccggtgc
    18241 tggggaagtt tgaaggccgc gacaaacgcc cggaagtccc ctcgacgtat gcaaacgggc
    18301 aattgatctg ggagcccgat ttgaagcggg tggatctcaa aggctacatc catgcgatgg
    18361 acaatgttgc cacgcgcggc gagggcggac gcggtctgcc cgtacacatc gcggacttgc
    18421 aggatgagcg gcacacacgt ccgtcgcgct tcaccatccc gatccacagt tcctatccga
    18481 tcatcggcgg gcgcttgtgg tgcacgctcg taaaggaggg gaactccagc ctcgaccagg
    18541 cggccatttt ttacggagaa cccggctggg gaggcggaaa cctgtatacg ttccagtggg
    18601 ggaagggatc aagagacgtc gagttcgatt tggaccccaa catcatgcga tccggtgtgg
    18661 tttataacta caccatcggc tttaacctgc gcgggaatgc caagtccgat ccgccgtcgc
    18721 aggcagggtt ggaacgagta cgagtggtga cggaccttca ggtctctccg cattcgctgc
    18781 ctgctttgtc gcttggaaaa aatctggtgc aattccgcga cgaatcggcg gcaggccggc
    18841 gggtccggat tacgcaccgc tggcgggaag ttcacgaccg ccgcccaccc gccgctatca
    18901 ccgtggcatc caccccgcgc gatggcgggg aagtgcaggc gcttgcgccg gttctgaaat
    18961 ggaatgcccc aacgaataat gcttcaggcg aaatcgtcga ttaccaggta atggtgagtt
    19021 tgcgcccgga ctgccgttgg ccgctttcgc ctacccttca ccagaacacc ggcacggcga
    19081 aaacagaatg gacggtgccg cagagttttc tgaatccagg cacaacgtat tactggaagg
    19141 tgcgcgctcg taacgttcat ggcgacgtcg gcccgtgggg ttccgtcttc cacttccgga
    19201 ccgctgcggc cgcgaaataa cccgagtgat ccaaagaggt ctgattcctc gcgttagtta
    19261 gtcgaagatt atctgggata ccgccggggc cgttccggtt gcggcggttg gcgtaagcat
    19321 ctattcgacg gagttgctgt tatgaagaag tgtgcgacgc tcctttttgt tgcggttctg
    19381 tttttacagg ctcagcacaa ccatcacgaa tccactcacc agaagccggt cgaattgctg
    19441 gaggggttgg gcgaccacac gcacccgatt gccacgaatt cgcagatggc ccagaagttt
    19501 ttcgatcagg gcctggcgct cgtgttcggt ttcaatcacg atgaagccgc ccgcttgttc
    19561 gcacgcgccg cggaactgga gccgaaatcg ccgatgccgc attggggaat cgcgctggct
    19621 ctgggaccga attacaacat gccgccaatg cccgaacggg aagagaaggc gtggcaggca
    19681 atagaaaaag cggtcgagct gtccaaacgg gcgccggaga acgagcgcgc ctatgtaaac
    19741 gcactggtga agcggtactc gaaagacccg gaagaagacc gcaagcaact cgcggtgcat
    19801 tacaaagacg cgatgaagga agtcatgaag cgctatcccg acgacctgga cgccgcgacg
    19861 ttttacgccg aagccttgat gaacctgcgg ccgtggcagc tatggagcaa agatggtgtg
    19921 ccggcggagg gaactctcga gattctggaa gtactcgagg gtgtgctgcg gcgcgacccg
    19981 cgccaccccg gcgccaacca ctactacatt cactcggtgg aagcgtcaaa gaacccggag
    20041 cgggcgctgc cgagcgcaat gcgtctcggt tcgctcatgc ctggcgcggg ccacatggtc
    20101 cacatgccgt cgcacatctt cctgcgcgtt ggcgattttg agcagtccgc catggtcaac
    20161 gagacggctt ccgaggtgga ccggaaatac atcgagcggt ctggcgccaa ggggatctac
    20221 ccgctgatgt actattcgca caacctgcac ttcgtatcgt atgcgcgcat gttgcagggc
    20281 cgcttcgagc aggcgttgga ttattcgaaa cggttgcgga aaaacgttga cggtgccatc
    20341 gacgaaatgc ccatgctggg gccgtacggt gctttcgaat ggctggtgct cacgcgattt
    20401 ggcaagtgga aggagatgct ggcggagccc gagcccagtg agaaacatcc gtttatgcag
    20461 gcaatgtacc ggtactcccg tgggctggcg ttcgccggtt tggggaatgt gccggaggcg
    20521 gaagcggaac gggaacgcat gggagctttt tgtacccgga ttcccgagag cgaaatgcta
    20581 atggtcaaca ccatgcgaag cgttctggat attggcatgg tggagctgga cgcgcgcatc
    20641 gcgcgcgcga aaggggattc agccgcggaa gttgcacact tccgccgtgc cgccgaactc
    20701 gaggaccagt tgaactatat ggagccgccg gactggcact acccggtccg tgaagcgctt
    20761 ggcggggccc tgttgcggca gggcaaagcg gccgaggcgg aagcggtttt ccgacgggat
    20821 ctggaactgc atccgcgcaa cggccgttcg ctgttcgggc tgatggaagc gatgaagatg
    20881 cagaagaagc aggtgagcgt agactggatt caaaaagaat ttcaggaggc ttggcgagac
    20941 tcgccaatga cgctcaaagt ggccgatctt taagccgtaa taagtttttt cctcaccccc
    21001 accctccgag gcgcgccggt ctttccggcg cgctattttt gtgtagccag ttccggcgac
    21061 aggctccccg ctgcctcata catcgccagg atgttcgccg gtggagtgtc ggcctggatg
    21121 ttgtgtgtgg ggctgaggat gtagccgccg ccggcgccga gaacggcgat atgctcccgc
    21181 gccgtctggt gtacctcgcc aggtgtgcat cggggaagcg tcgcctgtac atccatgccg
    21241 ccgtgaaagc agagcctgtc gccgaaggtg cttttgattt cttgcgggtt catgccttcg
    21301 gcgctgaatt gcagcacgtc cagcacatcg gttccgatat cgatgagcgc ggggatgaag
    21361 gggcgcacag cgccacagga atggtacatc acccgggtgc ccagttcgtg aacgcgccgg
    21421 ttgagacgct catgatgcgg cgcgacgagc ttgcgccaca tgcgcggcga catcagaat
//
LOCUS       DASTMK010000142        29139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_39442, whole genome shotgun sequence.
ACCESSION   DASTMK010000142 DASTMK010000000
VERSION     DASTMK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 29139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29139
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39442"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VFQ79_05915"
     CDS             complement(<1..1628)
                     /locus_tag="VFQ79_05915"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEU0139223.1"
                     /translation="MQVKKTKPAGFVRDPATGAMARLRAKYYCAGDPVDVVTGVNTDE
                     TLELELPGPIPFQWRRYYSSARNAEPLPLGWGHTHEYDCSFAADLDGYRFRGPTGLTY
                     FPYIPEDGGVAAANGLLLRRVTSLQFEIHCDARPVMEFVFHNPEAPAPLNRLIRGRYA
                     MVFEYTAAGALSQITGGGPHQLLEVQTDAAGRITLLSVLKDGAPRRLMEFEYDAAGNL
                     IRGTDAYSNSFYFAYDRANRMIRKTDRRGYSFEYEYDDSGRCIRSRGEDGLHDVQLRY
                     LPAERTTVVTRPDGGVWKYFYDDAGALTNIVDPYGGTQILKTDEDGRLVSVTGANGDT
                     SHILYSPEGLPLATVDPLQKATLTDAPEQQPVELPELLVSEPPLSRLATQALGHPPLF
                     WERKAADRTPLALEYGDWIDPEFLAPPTRALVETLVPEWRPFFVAVDSTSSDPSAWRK
                     TKETDFGPGTLDRDFAGKIFRYTDPAGHSEHWRYDSAGNVTHYRDRNGSQWFYEYTSW
                     NLLDREVDPLSGATAYEYTYAERISKCVDAGSTGT"
     gene            complement(1619..2563)
                     /locus_tag="VFQ79_05920"
     CDS             complement(1619..2563)
                     /locus_tag="VFQ79_05920"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEU0139224.1"
                     /translation="MRLPTVPPPSPPARPTNVMHDVASGIGAVVGAPMQVVNLANEGF
                     AKATNVIAQALPCFPAATLGSIAMGMPHGHLHPPSLVPPAPLVPLPPIGPVMFGTSVQ
                     VLINGMPAARCGDLGITPTCCGLPPIYEIFTGSSKVFIGGARAARALDVTYHCKPIPP
                     AGAAARGTAAAMKTSMKAAMMASAFAQKALMGAQIAGMAAQAAAIAGDMIEAVEADDA
                     AMSAALGMSAAMMAAQMASDAVGMAMGAAMGKDPCIPPGTLGAITLNTSPNVVIGGFP
                     MPSWLAIARGLLKIVKGLRARRARKPDALGSCGAPCCR"
     gene            complement(2560..3804)
                     /locus_tag="VFQ79_05925"
     CDS             complement(2560..3804)
                     /locus_tag="VFQ79_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139225.1"
                     /translation="MRKLFKALESELEEFVNQRDDLVMIASANDNDAPILLKLLRDIE
                     QKQGTDIFLLFADDFVQPGAYVSVIAERLREEHRMACEALKEEGKPPLRAMPDDIFNA
                     ALPPLDRLWKAICFARSLVPEDGGHRLVWVACPQKIADRREYLRLVTCFVPRGMVQPW
                     MRGVRLVFRDQPDSLGFAPGLARAPRTRVLPVDLGPAAMEKSMRDDVEDDGLPLEDRM
                     QSLLMLASLDNAHGRSDDAIAKYKVLLGHYQSTNNQPMQAFVLNAFGDMFQRKSELDR
                     AQYWYECAVPPVVESKDPVLMATVVKNLGEVSYKRRQFSDAEQYFDGLDRLAAHNLDP
                     ETKVQAMEWKGLSQEKQGRVREAVASWDGAATLCRSIGLPPLLRKILEHQERGYKSLG
                     ERDRARAVRAEIDASEREEVPA"
     gene            complement(3810..5837)
                     /gene="tssI"
                     /locus_tag="VFQ79_05930"
     CDS             complement(3810..5837)
                     /gene="tssI"
                     /locus_tag="VFQ79_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEU0139226.1"
                     /translation="MPASITQANRTISIDTPLGQDVLILRAFTGTEAVSQLFRFQIDL
                     VSDDAEIDYDAIIGRNVTIAVRLEDESTRRYFNGHVSRFTQLPSEGRLARYQAVVEPW
                     LWFLTRTSDCKIFQKKSVPDIVQEIFRTFGFQNFELQLNRSYTPWEYCVQYRETAFNF
                     ISRLLEQEGIFFFFRHEQGRHTLILADTPSAHKPCPDQPDARYHLVGGGGYAPGDDTV
                     SSWRMEQEIRPGKYALSDFNFETPRDSLLSSLASKIDQGGNSRYEIYDYPGEFANRRE
                     ADETVKLRMEEEEACHTTITGESSCRGFCSGFRFNLTGHPRSDQNGGYVLTSVTHSAN
                     TGSLLSESDGEGARYSNNFTAMPANAPLRPARITPKPMVQGPQTAIVVGPAGEEIFTD
                     KYGRVKVQFHWDRYGKRDENSSCWVRVSHPWAGKGWGAVSIPRIGQEVVVDFLEGDPD
                     QPLITGRVYNAEQMPPYGLPSGAVVSGIKTNSTKGGGGYNEMSMDDTKGKEKITIHAQ
                     YDMGTTVEHDDTQTVHNNRTIGVDGTHTETIKKDTTIKITEGNLVHDVEKGTATYHVK
                     DAVTENFDNTQTTTVKKAVEIWSTDATILVDAKTSIELHTGSSRMVMKSDGSIRIDGK
                     NIEIIGTQEVKLGVGTQTITLNNQEVASAGAGIKATAVGVHEISGALVKIN"
     gene            6098..6448
                     /locus_tag="VFQ79_05935"
     CDS             6098..6448
                     /locus_tag="VFQ79_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139227.1"
                     /translation="MSRHDKSRTDEAGLLQGTLDLLILKVLALEPLHGYGIAQRLKQI
                     SREALQIRQGSLYPALYRLEKRGMLKAQWRTAGGREAKYYSLTRAGHQQLQSETEGWE
                     RLCAAVRLVLETSR"
     gene            6457..9048
                     /locus_tag="VFQ79_05940"
     CDS             6457..9048
                     /locus_tag="VFQ79_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139228.1"
                     /translation="MWARFEKRLDAEIRFHLDELIQKYMKQGLTRDEARQRALREFGA
                     VELAKDEVRDLRLFDWLQQFGRDLRVAVRVARRAPAFTVAVIATLAIAIGSAVAVFGV
                     VYAVLLRPLPYPDPERLVSIENRTGKESKQDAVHMQQYQDWVSHASSFESVAAYNLFF
                     EHGTFNLTGLGEPERLRGINVTANLLPMLGARAVIGRLFAKGEDQPGAVPVVVLSYSF
                     WQRRFGAGSSIVGQQLIINDIPHIVAGVIAPELAYAGTLILSSGFDVYLPLIQGKQAY
                     RYGMFLAVVGKLRPGVSRQQAREAVEALHRASFGNSELRDMRQDVRLLSERVNSTLQT
                     PLWMLLGAVALLVFTGCANLANLFLARAAARTREFSVRAALGAGRSRLARQVLTECLL
                     LAGIGAGIGVALAAAILRYLRGAEWLQMPRLAEMAIHWPVVLFAVAACGLTAILFGLA
                     PALRVARAGVVSGLKEGARGTPGGGVRGGLRWALIVVQVSMSCVLLTGSGLLIRSLLK
                     LLEVNPGFQPEQLVAMRVDPGERREHGPKMTAFFENILERVRALPGVNSAALGVNLPL
                     DRNMRWGYTIRGQKEQSSLPPTAAVRMVSPGYFQTLGIRMYAGRDFSPHDNAQAPPVV
                     VVNRTLAREIGAVIKPLGSKLRIAGREHEVIAIVEDTKHEGLDRESGTEYYLALAQTL
                     PFPVVDVVVRSPLPVGSVSAAVRGAVWAVDRDQPVGKPQTLQALLDRSLSPRRFFTWI
                     LTAFAVFSALLAFAGVYGVVSYGVAQRTSEIGIRMALGATSSDVMRLFAAESIAVGFL
                     GLAFGVAGALAFARVLRSQLYGITASDTMTLCGAALLISGCVVAATLFPARRATRLDP
                     RQALGAE"
     gene            complement(9058..9396)
                     /locus_tag="VFQ79_05945"
     CDS             complement(9058..9396)
                     /locus_tag="VFQ79_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139229.1"
                     /translation="MRVFDHVGIPVVEKQPEEMYVEASKVWVTDPLRHPNRIEYLRYE
                     PDSPVSGPVRDLPHMAFRVENLEREMEGAEVLLGPFNPAGALRVVFVLKEGAVFEFME
                     NAADGHWFQR"
     gene            9465..10421
                     /locus_tag="VFQ79_05950"
     CDS             9465..10421
                     /locus_tag="VFQ79_05950"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0139230.1"
                     /translation="MRRREFLFSALAGGFAPAALPQQRDEPPEYTAHGSLVVERSLSG
                     KPHAGKVLAAIQPHSDDIPLFAAGTVAKLLNEGYTGHLIRVTNDDMAGPGTIGTTVLA
                     NEKDNDEVARVLGLKSIFNLNYPNHNMDGTSRPELRGRFIFLFRLLKIDTVISYDPWS
                     HYEENPDHYVTAACVEAACWMAGGSKDYPEHFAAGLKPHSVREKYYFARFDQRVNRVV
                     DIGSTVEKKIDANLVNVAQGPAGINGSKLRSMLAGRGQRLPLLGSDDTTANREYIREF
                     VLSRDAEIGQKYGLQYAEQFHYIGPGENRIEQYIKRNAVPLK"
     gene            complement(10393..11031)
                     /locus_tag="VFQ79_05955"
     CDS             complement(10393..11031)
                     /locus_tag="VFQ79_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0139231.1"
                     /translation="MAYRTTPKMAARKQERRQAILAVATRLFGRHGYHATTVPMIVAE
                     AGTSTGNFYFYFRNKEDIFAAALAGLAERISAATGAAAAKAPAAPLAQMKAAVEGFVL
                     FLAQNPGEARILIVESSGLSPRLEQIRRSVMASHACGVEKVLRRLAPKLPLSDSAIMA
                     RCWTGAVYEAVYSWLELPAKKRPPAKVLAGAVARFNLRAVGAPVTLTEQRCV"
     gene            complement(11035..11487)
                     /locus_tag="VFQ79_05960"
     CDS             complement(11035..11487)
                     /locus_tag="VFQ79_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139232.1"
                     /translation="MAVPETKSANGVGRAWLALCFALSLHVLDEASTGFLAVYNPTVM
                     AIREKLPWLPLPVFDFKLWIAGLIAAILLLFSLSPFVFWGARWTRPVSYAFAAFMTVN
                     ALGHITGTLLGSSGPMPGFYSSPFLLAASIYIFFQLRASRPRLPLTAE"
     gene            complement(11550..12500)
                     /locus_tag="VFQ79_05965"
     CDS             complement(11550..12500)
                     /locus_tag="VFQ79_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0139233.1"
                     /translation="MVAAVVALALAASGSNAAKLELRGQVQPSYPKMLANLSAQQFPF
                     DATVTCDKAGRFRFTKLASGAYTLSVFVRGEGLVRKSVQLDAAKADSKGVVHMDVAFK
                     VPAGDTSIVQYAKRTGTVPLWALQIPPKARREYRNARRALSEQRIEDTIRHLEKAVEM
                     SPEFAAAWQLLGQIAFQSDRLAEAETYFRRVLAQDPDALESIVNLGAILLNRNRCAEA
                     LPYSERAVRELPADAATNSQLGKNYLCLGDTVRALQYFSAAKKIDPGHYSKPQLALAM
                     IYMAMGADPLARTELDEALARSPDDSLAIDLKRKLASRAR"
     gene            12630..13193
                     /locus_tag="VFQ79_05970"
     CDS             12630..13193
                     /locus_tag="VFQ79_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139234.1"
                     /translation="MTPSILRDSPDAKWRLLELVLQAETHAGKPREQWPEIILIGDAF
                     APGTLAAARALAGAYEVQIRFAESLSGAPRFHEAPDLSAMGHFFSRGGLPHRRIFFTG
                     GHLRWSARARRRFAYVRQSGRFPDDFDFWRKRLSDPNSVRPSTRENRIQFRLHTQQDF
                     DRFEEAVRQLETANWTGLAADPDEDAG"
     gene            complement(13206..14780)
                     /locus_tag="VFQ79_05975"
     CDS             complement(13206..14780)
                     /locus_tag="VFQ79_05975"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HEU0139235.1"
                     /translation="MIFSSLKHRLIVSCQAPDGDPFRDPDSMARFAEAAALGGAAGIR
                     ANGPEDIRAIRARVDLPIFGIQKSIASDGRILITPSVESAQAVVEAGASIVAIDCTVR
                     GRRYGALERLKEIRDRLRVPVMADIATVEEAIRAVEAGADTVASTMRGYTDETAHVDR
                     FEPEFIAELVHSVPVPVLAEGRISTPEEAASALRAGAHAVIVGTAITAPRRIVEVFAG
                     AMDRERRRLVTGQVAGIDLGGTNTKYGVVASDGRLLSEGVAPTPWSAGREALLGHLKR
                     IARECVEGGGAAAIGIATAGWVDPSSGEVVYATGNLPGWTGAQIAAEVSEAVGLPVAV
                     ENDANALAVAEFRFGAARSARNFVVITLGTGLGGGCYIDGKLNHGSHFFANAIGHMPV
                     EPDGAPCTCGLRGCLEAYTNAAALLRRAPQFANAEQLIHAANGGDESARAAISAQAVY
                     LASGLAAVVQLLDPELLVLSGGLVQNNTLLLTRVREELSRRLTVWDQRHLEVCASGLG
                     YHGGVLGAAAVAFERC"
     gene            complement(14777..15760)
                     /locus_tag="VFQ79_05980"
     CDS             complement(14777..15760)
                     /locus_tag="VFQ79_05980"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEU0139236.1"
                     /translation="MIGLGVFYATLSGIANGLFSAPMKVIPVWKWENIWLVFIVTACV
                     LMPPIVVLPAADPSAVIAAAPTAAVWAALTFGFAWGFGAILFGLSVHTLGVSLANSLV
                     IGLSSALGSVIPLIVTGGLKAEPRVFVLFAGVAVFIAGVWLCGRAGRMSEQQTSREWR
                     GYFFAIGSGVMSAVFNIGYSLALPVADAGERLGLTRFTSTNVIWLLMLGAGAVPNIAY
                     CLQLLSRHRSAKLFNAGPPAKTWGLGLLMGLLWGGSIFLYGAATPLLGDIGPSIGWPL
                     SLAVGLLVANLMGYLLGEWRTAPLAAVRTMQFGIVTLLIAIVITAASTTLS"
     gene            complement(15794..18346)
                     /locus_tag="VFQ79_05985"
     CDS             complement(15794..18346)
                     /locus_tag="VFQ79_05985"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HEU0139237.1"
                     /translation="MNRVLFIVFAAMFPVSALYSQKTDLYDGKVFGVFEPLNVTETPK
                     TITITGGTFRYVVDRSIGQIVSAKALNDEFVAAGTSFPNPYIGVMPDDDPGARREGGV
                     DRPRFGYEKSVEMRPRLWSGDLTDAFRFDAAKGTGIRTELVRANAELVEVRATGSYGS
                     TPLSWQIDYLFDVDGFTKVTVSLTTSRPVQLRWNCFNHAFLAKEPIQYLTKVSDPKSP
                     PIDVRPEPTVSIAAAERDQPVFESHWNAFFHLANRVTGIEFSKQDFADRWSGYRDSSV
                     ILESGKRVDTGAVETVDGRELRGWDSRGRVNVFTQLYRRADALELEEFDIRNATYPLN
                     PGDVRKRVFWVQTTPAKHPRNDLNSIRAVWPGPHQIVMARWRGTREPWQPPTDEQVKL
                     WAQMGVNLIIGGANYWSGDYARPLEAEKIRHFLRTAHQYGIKVIPYVTFTDFNFAAPA
                     YQQHAAEWMASAGIEYANETTLMCYNAEGWRNHLEKQWDQLLADFDFDGLYIDHWINI
                     RFCNNARHGCGGYLGSFATEGYHDFAKRARRVVARHTAGKGVMLLNANMLLFSGMVPW
                     FDIRLNGENDDPIKMREETIVTTWNGWGQGVQSLAMWRPNQDTRTMINLLTTFMIPFR
                     LNRAIAPKTVEEWRVSKEPEVALARELWGYWRFFGLNGAEKYSSFGTADVLAMSQPGS
                     IVNGFARDGRALIVMGVLGGSGKRSEPLKILAPEKLGLERGSRYRIADLRRNRYLSDR
                     AYTTPELAQIPVTLEGDDPLILFIEPERKGPRLVYFSGADGVEASLSGGKLVLKVSAV
                     PGSPIELHVDAAGREFRAATPGFERAPVSGDFAVFTGSVPPGGIVTLSPSIQ"
     gene            18387..19517
                     /locus_tag="VFQ79_05990"
     CDS             18387..19517
                     /locus_tag="VFQ79_05990"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0139238.1"
                     /translation="MRVIGLAMCGVLLAGVCATAQLRRDVPSAAGMSAERLNRASALL
                     AAEVESGRVGAASLLVARRGRIVFHKGFGVPADAVFLLASITKPVTACALMLLVERGQ
                     VGLNDPVNLYFPEFTGDEREKIRVRDLLSHTSGLPDMLPENIELRRSHAPLSEFVNRA
                     LKTPLLFPPGTGFRYQSMGTLLAGTIVEKVTGQPLREFEKKEMFEPLGMKDSSLGLGG
                     RKVADLVPCMTSPNANKADEERFGANSDYWRDMGHPWGGMHSTTADLAILLQTFLDGG
                     TYGGTRLFSPATVAQMTRNQNEGLNDPWGLGWSLAPSRAWNALGDLVSARAFGHAGAT
                     GTVAWADPETKLLCVILTNRSYGSDNGRLLRRVSNAVAAAVE"
     gene            19561..20805
                     /locus_tag="VFQ79_05995"
     CDS             19561..20805
                     /locus_tag="VFQ79_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0139239.1"
                     /translation="MSFHENRRAFLKTVAALPLLARASMAKPPKTNVYTRIGVRPLIN
                     ARGTWTYLSGSLELPEVRAAKHEASLQFVDMFELQRGVGKRLAELSGAESGMVTSGAA
                     GAMAAATAACIAGTDPKNVWQLPDTTGLKHEVVMFGGRSAFDNAIRLAGGKLVLTRSF
                     EELEAAINDNTAMVYTTADGERLEKALAITKRRGVPMLLDQAAGIPPIENLSRYATMG
                     VDLYTFSGGKGLAGPQCSGLLLGRKDLIEAALANYNPWEGAVCRPMKVGKEEIMGCLA
                     AVEAWKKRDLASLDREWGERVKRIAKMVETVAGVTTDIQIPQGGNRYPTLTVNWDAEA
                     WGFTVQDCALQLRDGEPRIEALTNDNPSLVPAVREGYPKADPKRSARPDRLRIVPSTL
                     QPGEDLIIGKRLRQILGSARKG"
     gene            20844..21749
                     /locus_tag="VFQ79_06000"
     CDS             20844..21749
                     /locus_tag="VFQ79_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0139240.1"
                     /translation="MKDKKSVQDPKRKDPKKQGAKPQFSQKKIKVPGLEREMSPKADH
                     GEESYEGLGRLKDRAALITGGDSGIGRAIAIAYAREGADVLISYLPEEKEDGEETARW
                     VEMAGRRAVKVPGDIRKERHCQALVDRAFKEFGKLDVLVNNAGFQMSHDRIEDFTTEE
                     FDRTFKTNVYAMFWLSKAALPRMKAGSAIINTASIQSFDPSPNLLAYAATKGTIVNFT
                     KGLSKMAMQQGVRVNAVAPGPVWTPLIPSTMPKEKVKSFGEHTLFERPAQPVEIAPVF
                     VFLAGNESRFVTGEVYGITGGQTPY"
     gene            complement(21724..22545)
                     /locus_tag="VFQ79_06005"
     CDS             complement(21724..22545)
                     /locus_tag="VFQ79_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0139241.1"
                     /translation="MAKTIVLLLIVAACWALLSFLAHRSIYYPLRDGWWEQKDRVGAE
                     DVWIDTTDGVRLHGWWIEAPGNGLATLYLHGNGGNITHRSHRVPAITSAGSSLLLLDY
                     RGYGKSRGAPGEAGLLRDAQAGYEFLLSRGIPPERIVLYGESLGTAVAVSLAAVRPPA
                     GLVLEAPFTSARAVAARVLPIIGPLLISGYDSLKTITRVHVPLLVLHGDQDEVIAFEF
                     GERLFAAANGPKFFWRVRGAGHNDIPETAGPEYVERLRAFYAKLRPPLNTESGPR"
     gene            22931..23134
                     /locus_tag="VFQ79_06010"
     CDS             22931..23134
                     /locus_tag="VFQ79_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139242.1"
                     /translation="MGPTECFPNPAGDNEEKPFTLLPPADRGEVLHEQLRYLMEHPRG
                     CAEDCAECQRLRSVADLLMAAFR"
     gene            complement(23240..26431)
                     /locus_tag="VFQ79_06015"
     CDS             complement(23240..26431)
                     /locus_tag="VFQ79_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139243.1"
                     /translation="MYSRNVLCIAALAAILPFSYLHAQVSTATILGTVTDRSGAVVPN
                     ARVTVTNLETNFSRSMDTDQLGQYSIPFLPTGTYSVEVVTSTFKKFVQTGVVLDVGRS
                     ARVDPVLDIGALTEVVSVTADAPLVNTTSAVVGRTVENTEIINLPLVNRNVYSLLNLT
                     PGVEASESGNAFGFREQRTMVNGGSYGGSGTVNYFLDGGNNTTGLRGTGNAVPNPDAV
                     QEFRVITNSYGAEFGRFAGGVIDVVTKSGTNEFHGSLFEFIRNDKLNATTWNASSKAP
                     LRRNQFGGTLGGPVLRDRTFFFGSYSGLRQREYEFKNTAIVPTSLERAGNFSQSAIQP
                     VDPATGQPFPGGVIPSNRFDPTAKRILDEYIPQANLPGNFVEVTQPEPYDTDELQFKV
                     DHMLSQSHQLTGSYFRDTGTSLESLAGNLIWSQRQFKWTQQNFNVSDTWTVGPTAVNQ
                     LRLTYVRNFGGRLNIPEISLGDLGSGFQIQGAPSLPQIQVSGYFNLTQAIAGPSAGSN
                     YYGLRDNFSLNRGNHTLKFGGEVYLEKFIHDTTLNNYGTFSFNGTISKNALADFLLGR
                     PVTMNQDAPVTKIDNVWYTGLFIQDDWRIHPRLTLNLGLRYDFQTPATDPHDRKLTFI
                     QGRQSTVVPNAPVGLLFPGDEGIGRGIISSDRNNFAPRIGLAWDPFGDSKTSIRTGFG
                     LFYGGISGNEWNSTADNQPFTIRQRFNNVASLTDPYGLLPGGVSPYPYSYTSSAPQFI
                     FPASIAGPSLDFRWPYTYQMNFSVQRQLSSDLSVEAGYVSSLSHKLPFARDLNYPVYG
                     AGATTGNVNNRRPIQPGTLAVITELNSIMNSAYHGLQLTADKRMSRNFQVKAFYTFSK
                     SLEGARMQNDTTSGGAQNMNNLAAERGRTNNDRRHNFVASIVWQTNYFHDVRYAHLLF
                     DGWTISAITTFRSGAPFTVTNGTDRNLDGDSGDRANLVGNPRLDPNRSRDAVTAMWFD
                     PSAFAVTPLGQDGTSGRNILDEPGRRNVDFAVFRDFTIRENVALQFRAELTNGFNLVN
                     LNAPTSNLNSSAVGTIRAAQDMRQVQLGLRLRF"
     gene            complement(26559..27362)
                     /locus_tag="VFQ79_06020"
     CDS             complement(26559..27362)
                     /locus_tag="VFQ79_06020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0139244.1"
                     /translation="MILQLDEFKTKQRAIWEAGEYSALSPYIADVAERIVAKAGIEPG
                     MKVLDVACGTGNAARIAARAGARTTGLDFVPKLLQAGKSKAEAEGLEMEWREGDAENL
                     PFEDGSFDRVLSTFGHMFAPRHKRTAEEMARVCRRGGAIVTATWTPEGTVGHIFKVSA
                     AYMPPPPGYALPPILWGSEEHVRELFAGTAAGFEFERHVNIIQWDSLDAWTDFFMERF
                     GPMVMARTMLGDRFGELRQHVVEIWRNANQAKDGALRLPQEYLVSLIRL"
     gene            complement(28197..28400)
                     /locus_tag="VFQ79_06025"
     CDS             complement(28197..28400)
                     /locus_tag="VFQ79_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139245.1"
                     /translation="MSVARKITVEVPAELLERAQQASGTGITRTARTGLQLIAASQNY
                     AQLRALRGKVRFLRTAAKLKRDR"
     gene            complement(28482..>29139)
                     /locus_tag="VFQ79_06030"
     CDS             complement(28482..>29139)
                     /locus_tag="VFQ79_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0139246.1"
                     /translation="RIRLRGLKPELREIVGVVRDAKHYGAREHTWDMTYLPGAKEGSF
                     LVRVHEHTSLLSSDIREAVSAAGSTAQVEHIRQLPAIVEGTFSRERLIATLSTAFGAL
                     ATLLACIGLYGVMAYHLSRRTGELGIRMALGAQPSHIKWLAFRETLVLVVVGSAIGIV
                     GAVAATHYVSSMLYGVKPAEGSVVAGCTLLLSAVALAAGFVPARRASRIDPVTALRHE
                     "
BASE COUNT         5823 a         8708 c         9008 g         5600 t
ORIGIN      
        1 gtcccggtgc tgcccgcgtc gacgcatttt gagatccgct cggcgtaggt gtactcgtaa
       61 gcggtggccc cactcaaagg gtccacttcg cggtccagca gattccagga agtatactcg
      121 taaaaccatt gcgaaccgtt ccggtcccgg tagtgcgtaa cgtttccggc gctgtcgtag
      181 cgccagtgct cggaatggcc cgccggatcc gtgtaccgga agatcttgcc ggcgaagtcg
      241 cggtccaagg tgcccggacc gaaatcggtt tccttggtct ttctccacgc ggacggatcg
      301 ctggaggtgg aatcgaccgc tacgaagaaa ggccgccact ccgggacgag agtttccacc
      361 agggcacgcg ttggcggcgc gaggaattcc ggatcgatcc aatcgccata ttcgagcgcc
      421 agcggcgtcc ggtccgccgc cttccgctcc cagaacaaag ggggatgccc tagcgcctgc
      481 gttgccagcc gggacagcgg cggctcggaa accagcaact ccggaagctc aaccggttgc
      541 tgctccggcg catcggtcag ggtggctttc tggagcgggt caacggttgc caagggcagt
      601 ccttcgggac tgtagagaat gtggctggta tcgccgttcg cgccggttac cgacacgaga
      661 cggccgtcct cgtccgtttt gaggatctgc gttcccccgt acggatcgac gatgttcgtc
      721 aacgcgcccg catcgtcata gaagtacttc cagactccgc cgtcgggccg ggtgacaacg
      781 gttgtacgtt ccgccggaag gtaacgcaat tggacgtcgt gaaggccgtc ttcaccgcgc
      841 gaacgtatac agcggccgct gtcgtcgtat tcatactcga aggagtagcc gcggcggtcc
      901 gtcttccgga tcatgcgatt cgcccggtca taggcgaaat agaaagaatt gctgtaagca
      961 tcggtcccgc ggatcaggtt gcccgcggcg tcatactcga attccatcag ccgccgtgga
     1021 gcaccgtcct tcaggacact cagcagagtg atccgtccgg cggcatcagt ttgcacctcg
     1081 agcagttgat gcgggccgcc gccagtgatt tgcgacaggg ctccggcggc ggtgtactcg
     1141 aagaccattg cgtaccggcc acggatcaga cggttgagcg gagccggcgc ttcggggttg
     1201 tgaaacacaa actccatcac tggccgggca tcgcaatgga tctcgaactg aagagaagtt
     1261 acacgccgca ggaggagtcc gtttgccgcg gccactccac cgtcttccgg aatgtacggg
     1321 aaataggtca gcccggtggg accgcggaaa cgatatccat caagatcggc ggcgaaagaa
     1381 cagtcgtatt catgagtgtg tccccacccg agcggcagag gctcggcgtt tcgggcgctg
     1441 ctgtagtaac ggcgccactg aaacgggata ggaccgggta gctccaactc gagagtctcg
     1501 tcggtgttga cgccggttac cacatcgacg ggatcgcctg cgcagtaata tttcgcgcga
     1561 aggcgggcca tggcacccgt tgccggatcg cgtacaaaac cagccggttt tgtttttttt
     1621 acctgcaaca cggtgctcca cagctcccga gcgcgtccgg cttgcgcgcg cgcctggccc
     1681 gcaaaccctt gacgattttg agaagccctc tggcgatcgc cagccagctt ggcatgggga
     1741 agccgccgat cacgacgttg ggtgaggtat tcaatgtgat agcgcccaac gtgcccggag
     1801 gtatgcaggg atctttgccc atcgccgcgc ccatcgccat gccaacggca tcggaagcca
     1861 tttgcgccgc catcatcgcc gcgctcattc ccagcgccgc cgacatggct gcatcatcgg
     1921 cctccacggc ttcgatcata tcgcccgcaa tcgcggcggc ctgtgccgcc atccccgcga
     1981 tctgcgcgcc catcagagcc ttctgggcga acgcactcgc catcatcgcc gccttcatgg
     2041 aagtcttcat ggcggcggcc gttccgcgcg cggcggctcc cgctggtgga atcggcttgc
     2101 agtgataagt tacgtcgaga gcacgggcgg cgcgcgcccc accgatgaac actttcgagg
     2161 aacccgtgaa gatctcgtaa atgggcggca acccgcagca ggtgggcgtg atgcccagat
     2221 cgccgcatcg cgcggccggc atcccgttga tgagaacctg caccgatgta ccgaacatca
     2281 ccggaccgat cggcggcagc ggcaccagcg gcgccggcgg aaccaggctt ggcggatgca
     2341 gatggccgtg cggcatgccc atggcgatcg agcctaaagt ggccgccggg aaacagggca
     2401 gcgcctgtgc gatgacgttg gttgctttcg caaatccctc gttggccagg ttcaccactt
     2461 gcatgggggc gcccaccact gctcctatac cgctcgccac gtcatgcatc acgttggtcg
     2521 gacgcgccgg aggcgaaggc ggcggcacag tgggcagcct catgcaggca cctcttcgcg
     2581 ctcggatgca tcgatctccg cccgcaccgc ccgcgcgcgg tcccgctcgc caagagattt
     2641 atacccgcgt tcctggtgct cgagaatctt cctgagcagc ggcggcaaac caatgctgcg
     2701 gcacagcgtg gccgccccat cccaacttgc caccgcttcc cgtacacggc cctgcttctc
     2761 ctggcttaac cctttccact ccatggcctg tacttttgtt tcggggtcca ggttgtgcgc
     2821 ggccagccgg tcgagaccgt cgaaatactg ttcggcatcg gagaactgcc gcctcttgta
     2881 agacacttcg cccaggttct tgaccacggt cgccatcagc accgggtcct tcgattcgac
     2941 aaccggtgga accgcgcact cgtaccaata ctgcgcccgg tccagctcgc ttttccgctg
     3001 gaacatgtcg ccgaatgcat tcaggacgaa cgcctgcatc ggctggttgt tcgtgctttg
     3061 atagtggccg agcaacacct tgtacttggc gatggcatcg tccgaccggc catgcgcgtt
     3121 atcgagggaa gcgagcatca gcagcgactg catgcgatct tcgagcggca acccatcatc
     3181 ttccacgtcg tcgcgcatac tcttttccat cgccgccgga ccgaggtcga cgggcaggac
     3241 gcgcgtgcgc ggcgctcgcg cgagccccgg cgcaaaaccg agagagtccg gctgatcgcg
     3301 aaacaccagg cgcactccgc gcatccaggg ttggaccatt ccacggggaa caaaacaggt
     3361 aaccagccga aggtattcgc ggcgatcggc gatcttctgc gggcacgcca cccacaccag
     3421 cctgtgcccg ccgtcttcgg gaaccaggct gcgcgcaaaa cagatggcct tccagagacg
     3481 gtccagcgga ggcagggcgg cattgaagat gtcgtccggc atggcgcgaa gcggaggctt
     3541 cccctcttcc ttcagggcct cacacgccat gcggtgctct tccctcaacc gttcggcgat
     3601 gaccgatacg tacgcgccgg gctgaacgaa atcgtccgcg aaaagcagga agatgtccgt
     3661 gccctgtttc tgttcgatgt cgcgcagcag cttgagcaga atgggagcgt cgttatcgtt
     3721 tgcggaagcg atcatcacca ggtcgtcgcg ctgattgaca aactcttcca gctcactttc
     3781 gagcgctttg aaaagcttgc gcatgacact cagttgattt tcaccaacgc tccgctgatc
     3841 tcatgcacgc cgacggcggt cgctttaatg cccgcaccgg ctgacgccac ttcctggttg
     3901 ttcaaggtga tggtttgcgt tccaactccc agcttcactt cctgcgtgcc gatgatctcg
     3961 atgtttttgc cgtcgatacg gatggagccg tcgctcttca taaccatgcg gctcgacccg
     4021 gtgtggagtt cgatgcttgt ttttgcgtcc accaggatgg tggcgtccgt ggaccagatc
     4081 tccaccgcct ttttgaccgt ggtggtttgc gtgttgtcga agttctccgt caccgcatct
     4141 ttgacgtggt aggtagccgt gcctttctcc acatcgtgca ccaggttgcc ttcggtaatt
     4201 ttgatggtgg tgtctttctt aatggtctcg gtatgcgtgc cgtccacccc gatagtgcgg
     4261 ttgttgtgca ccgtctgggt gtcgtcgtgc tcgacagtgg ttcccatgtc gtactgcgcg
     4321 tggatggtga tcttctcctt ccccttcgtg tcgtccatgg acatttcgtt gtaaccaccg
     4381 ccgcccttgg tgctgttggt cttgatgccg ctgaccacgg cacccgacgg aagtccgtaa
     4441 ggaggcatct gctcggcgtt gtaaacgcgt ccggtgataa gaggctggtc agggtcgcct
     4501 tcgagaaaat caaccaccac ttcctgcccg attcgcggta tcgaaacggc gccccagcct
     4561 tttcccgccc atgggtgcga tacgcgaacc cagcaggagc tgttctcatc ccgtttgccg
     4621 taacggtccc aatgaaactg cactttgacg cggccgtatt tgtcggtgaa gatctcttcg
     4681 cccgcgggcc ctacgacaat ggcggtctgc ggcccttgaa ccatgggctt tggcgttata
     4741 cgtgctggcc gtaaaggcgc gttcgccggc atggcggtga agttgttcga ataacgggcg
     4801 ccttcgccat ccgattcgct caacagcgaa cccgtattgg cgctgtgcgt gaccgaggtg
     4861 agaacgtagc ctccgttctg atcgctcctc ggatgtccgg tcagattaaa gcgaaacccc
     4921 gaacaaaatc cacggcaact gctttcgccg gtgatcgtcg tatggcacgc ttcctcttct
     4981 tccatgcgca gcttcaccgt ttcgtcggcc tcccggcgat tggcgaactc gcccggatag
     5041 tcgtagatct catagcggct gttgccaccc tggtcgattt tgctggcgag cgaagagaga
     5101 agactatcgc gtggggtctc gaaattgaag tcggacaggg cgtacttgcc ggggcgtatc
     5161 tcctgttcca tgcgccagga cgagaccgtg tcgtcccctg gagcatagcc gccgccaccc
     5221 accagatggt atcgcgcatc gggttgatcg ggacagggct tgtgggccga gggggtgtcc
     5281 gccaaaatca acgtgtgacg cccctgctcg tgccggaaga aaaagaaaat gccttcctgc
     5341 tccaacaacc gggagatgaa attgaacgcg gtctcccggt actgtacaca atactcccac
     5401 ggagtgtagc tccggttcag ttgcaactcg aaattctgaa agccgaaggt gcgaaagatc
     5461 tcctgaacga tgtcgggtac ggattttttc tgaaagattt tacagtcgct cgtgcgggta
     5521 agaaaccaca gccacggttc cacaacagcc tgataccggg cgaggcgccc ctccgaagga
     5581 agctgcgtga acctgctgac gtgtccgttg aaatagcgcc gggtggattc gtcctccagc
     5641 cggacggcaa tggtgacatt gcgtccgata atggcgtcat agtcgatttc tgcgtcatcg
     5701 gacaccagat cgatctggaa ccggaatagc tgcgacaccg cctccgtgcc ggtaaacgcc
     5761 cgtaggatca gaacgtcctg gccaagaggc gtatcgatcg atatggtccg gttcgcctgc
     5821 gtaatggacg cgggcataaa acactcccct cactcgtaat acgcgtaatt aacgaatgaa
     5881 atgcgtcacg gcggctaagt ggttaacagc acagcagtgc acggcatcat aagagcatct
     5941 acatacagcc aaggatactt gaaattttga agacgggagc tttcagcgat cggcggtcag
     6001 tgttcagctt gcgggaacca ccggcgggct cgagctgtcg gggcagccac ttttatgcct
     6061 tgacatccta ggcaatcgcc agtaagctat ggttcgaatg tctaggcatg acaaatcacg
     6121 cacggacgag gccggtttac tgcaaggcac gctcgacctt ctcatcctaa aggtgctcgc
     6181 cctcgaacca ttgcacggtt atggcattgc gcagcgtcta aaacagatct ctcgcgaagc
     6241 cttgcagatc cggcagggtt cgctgtatcc ggccctttac cggctggaaa agcgtgggat
     6301 gttgaaggct cagtggagaa cagccggtgg acgcgaagca aagtattaca gccttacccg
     6361 ggcaggtcac cagcagttgc agtcggagac cgaaggatgg gaacgcctgt gcgcggcagt
     6421 tcgcctggta ctggagacgt cgaggtaggt ggaggtatgt gggcacggtt tgaaaagcgg
     6481 ctggacgccg agatcagatt ccacttggac gagctgatcc agaagtacat gaagcaaggg
     6541 ctcacacgcg atgaagcgcg ccaacgcgcc ttgcgggagt tcggggccgt ggaactcgcc
     6601 aaggacgaag tgcgtgatct ccggctgttt gactggctcc agcaatttgg acgggatctt
     6661 cgcgtcgccg tacgagttgc acgccgggcg ccagcgttca cagtggcggt gattgcaacg
     6721 ttggccattg cgatcggctc cgcagttgca gttttcggtg tcgtatatgc ggtcctgctg
     6781 cggccacttc catatcccga tccagaacgg ctggtctcca ttgagaacag aacgggaaag
     6841 gaatccaagc aggatgccgt ccacatgcag cagtatcagg actgggttag ccacgcttcg
     6901 tcgttcgaat ccgtcgccgc ttacaatttg tttttcgaac acggcacatt caatctgacg
     6961 ggcctcggcg agccggagcg tctccgggga atcaatgtga cggcaaatct gctcccgatg
     7021 ctgggcgctc gcgctgtcat aggccgcctc ttcgcaaaag gagaggatca acccggcgcc
     7081 gtccctgttg tggtccttag ttactcgttc tggcagcggc gattcggagc cggctcctca
     7141 atcgtaggcc agcagctcat catcaatgac atcccgcaca ttgtggcggg cgtgatcgcc
     7201 ccagaactcg catatgcggg aacgctcatc ctgtcatccg gcttcgatgt atatctgccc
     7261 ctcattcaag ggaaacaagc gtatcgttat ggcatgttcc tcgctgtcgt gggcaaactg
     7321 cggcccgggg tctcgcggca gcaagcccgc gaagcagtcg aagcgctgca cagggcttca
     7381 tttggcaact cagagcttcg ggatatgcgg caggacgtgc ggctcttgtc cgaacgcgtc
     7441 aactccacgc tgcaaacgcc gttatggatg ctcctgggcg cggttgcgct cctcgtgttc
     7501 acgggctgtg ccaacctggc gaatctgttc ctggcgcgtg cggctgctcg aacccgcgag
     7561 ttctctgtgc gcgcggcatt aggcgcggga cgcagcaggc tcgcgcggca ggtactcact
     7621 gagtgcctgt tgctcgctgg aatcggcgcg gggatcggcg tagcgctcgc tgctgccatt
     7681 cttaggtatc ttcgtggcgc agagtggctt cagatgcccc gcctcgcaga gatggcgatc
     7741 cattggcccg tggtcctgtt tgcggtggcc gcctgtggat tgacagctat tctcttcggg
     7801 cttgcaccgg cattgcgtgt ggcgcgcgcc ggcgtggtca gcggattgaa ggaaggcgcc
     7861 cgcgggacgc cgggcggagg ggttcgtggc ggtctgcgtt gggcattgat agtcgtccag
     7921 gtaagcatgt catgcgtgtt gctgacgggc tcgggcctgc tgatccggag cctgttgaaa
     7981 ctgctcgagg tgaacccggg attccagccg gaacaattgg ttgccatgcg tgtagaccct
     8041 ggagaacggc gcgaacacgg tccgaaaatg acagcattct tcgaaaacat cctggagcgg
     8101 gtccgcgcgc tacccggcgt gaactccgct gcgctcggcg taaatcttcc cttagaccgc
     8161 aatatgcgct ggggctacac gatccgcggt cagaaagagc aaagcagcct ccctcctacc
     8221 gcagcggttc gaatggtgag ccctggctat tttcaaacgc tgggcatccg gatgtatgcc
     8281 ggccgcgact tcagtccgca cgacaacgcg caggctccac cggtcgtggt tgtgaaccgc
     8341 acattggctc gcgaaattgg cgcggttatc aaaccattgg gatcgaaatt gaggatcgca
     8401 ggccgcgagc acgaagtgat tgccatcgtc gaggacacga agcacgaggg gctcgatcgt
     8461 gagtcgggta ccgaatacta cctggcgctg gcgcaaaccc tgccgtttcc cgttgtcgat
     8521 gtcgtcgtgc gatccccgct accggttggc tcagtctcgg ctgcggtgcg aggcgcggtc
     8581 tgggctgtag accgggacca acccgtgggc aaaccgcaaa cgctccaagc gctgctcgat
     8641 cgctccctct cgccccggcg attcttcacc tggattctga ccgctttcgc ggtcttctca
     8701 gcattattgg cgtttgccgg ggtgtatggt gttgtttctt acggtgtggc acagcggaca
     8761 tccgagatcg gaatccgaat ggcattgggc gccacaagta gcgatgttat gcggttattt
     8821 gccgccgaat ccatagccgt ggggtttttg ggactcgcgt ttggggtagc tggggcactc
     8881 gcgttcgcgc gcgtgcttcg cagccaactc tacgggataa ctgcttcgga cacgatgaca
     8941 ctttgcggcg ccgcattatt gatatcgggt tgcgtagtgg cagcgacact ctttccggcg
     9001 cgacgcgcca cgagattaga cccgaggcag gctcttggtg cagagtgata caccggctca
     9061 gcgctggaac cagtgcccgt cggccgcatt ctccatgaac tcgaagaccg ctccttcttt
     9121 gagaacaaac accactctta gcgcgccggc gggattgaac ggacccaaca gcacttcggc
     9181 tccttccatc tcccgctcga ggttttctac ccggaaggcc atgtgtggga gatcgcggac
     9241 cggaccgctg acgggactgt ccggttcata ccgcaggtac tcgatccggt tcgggtggcg
     9301 gagcgggtct gtcacccata ccttgctggc ttcgacgtac atctcttcgg gctgtttttc
     9361 gacaaccgga attcccacgt gatcgaacac tcgcataggt actactttag tccggtgaat
     9421 cgcaggttgc acccgcccat cgtttcccct tatgatcgac tcccatgaga aggcgcgaat
     9481 ttctgttttc cgctctcgcc ggtggcttcg ctccggccgc cctgccgcag cagagagacg
     9541 agccgcccga atacacggct catggcagcc tggtcgtcga acgttccctg tccggaaagc
     9601 cgcacgccgg caaggtgctg gccgccattc aaccgcactc ggacgatatt ccgctgttcg
     9661 cggcgggcac cgtcgccaag ctgctcaacg agggctacac aggccacctg atccgcgtaa
     9721 cgaatgacga catggcgggg ccgggaacca tcggcacaac ggtgctggcc aatgaaaaag
     9781 acaacgacga agtcgcgcgc gtcctggggt tgaagagcat tttcaacctg aattatccca
     9841 accataatat ggacggcact tcacggccgg agcttcgcgg ccggttcatt tttcttttcc
     9901 ggctgctcaa gatcgatacg gttatcagct acgatccctg gagccactac gaagagaacc
     9961 ccgaccatta cgtcaccgcc gcctgtgtgg aagcggcgtg ctggatggcc ggaggcagca
    10021 aggactatcc ggaacatttc gccgcgggtt tgaagccgca ctccgtgcgc gaaaagtact
    10081 acttcgcccg gttcgaccag cgcgtgaacc gggtggtgga tatcggctct acggtcgaaa
    10141 aaaagatcga cgcaaacctg gtgaacgttg cgcagggccc ggctggaatc aacggctcca
    10201 agcttcgcag tatgctggcc gggcgcgggc agcggcttcc gctgctcggc agcgacgaca
    10261 ccaccgcgaa tcgcgaatat attcgggaat ttgtgctttc ccgcgacgca gagatcgggc
    10321 agaagtacgg cttacagtat gccgaacaat tccactacat cgggccgggc gaaaaccgta
    10381 tcgagcagta tattaaacgc aacgctgttc cgttaaagtg acaggtgcgc caacggctct
    10441 gaggttgaat cgtgcgacgg ctccggcaag aactttcgct ggaggacgct tcttcgcggg
    10501 aagctcgagc cagctatata ccgcttcata gacggcgccg gtccagcagc gggccataat
    10561 cgcggaatcg gacaaaggca acttcggcgc gagccggcgc aggacctttt cgacgccgca
    10621 ggcatggctg gccataacgg agcggcggat ctgctccagc cgtgggctga gcccggagga
    10681 ttcgacaatc agaatacgcg cctccccggg attctgcgcc aggaacagca cgaagccctc
    10741 gacagcggcc ttcatctgcg caagcggggc ggcaggcgct ttcgccgcag ccgcgccggt
    10801 tgccgccgaa atgcgttccg cgaggccagc gagagcggcg gcaaagatgt cttccttatt
    10861 gcggaaatag aaatagaagt tgcccgtgga cgtgccggct tctgccacga tcatagggac
    10921 cgtggtcgcg tgatatccat ggcggccgaa gaggcgcgtg gctaccgcaa gaatagcctg
    10981 gcggcgctcc tgcttgcgcg cagccatctt cggggtggtg cgataggcca tgcgtcactc
    11041 tgccgtgaga ggcaaccggg gccgcgaggc ccgcaattgg aaaaagatgt agatcgaagc
    11101 agccagcagg aagggcgacg agtagaatcc gggcattggg ccggagctgc cgagcagagt
    11161 acccgtgata tgtcccaggg cgttaacggt catgaaagcg gcgaaagcgt agcttaccgg
    11221 ccgtgtccaa cgcgcacccc agaatacgaa tggcgacaac gagaacagca gcagaatcgc
    11281 ggcgatcaga ccggcaatcc agagcttgaa atcaaatacc ggcagaggca gccacggcaa
    11341 cttctcccgg attgccatca ccgtagggtt gtagacggcc agaaatcccg tagaggcctc
    11401 gtccaaaaca tgtaggctga gcgcgaaaca aagcgccagc caggcccggc ccaccccgtt
    11461 tgcggacttc gtttcaggaa cggccattgg catatacctc cctcgttcca aggatagaat
    11521 gacgtgtcat tctatccttg tcaagggaat taccgggccc gcgatgcgag tttccgcttc
    11581 agatcgatgg cgaggctgtc gtcaggagac cgtgccagcg cttcgtccaa ctcggtccgg
    11641 gcaagcggat cggctcccat cgccatgtag atcatcgcca gtgcgagttg gggtttcgaa
    11701 tagtggccag gatcgatctt cttggccgct gagaagtact ggagggcgcg gaccgtatcg
    11761 ccgaggcaga ggtagttctt tcccaactgt gagttggtcg ccgcatccgc ggggagttca
    11821 cgaacggccc gctcgctata aggcagcgcc tccgcacacc ggttccggtt gagtagaatc
    11881 gcgccgagat tgacgatgct ttccagcgcg tccgggtcct gcgccaggac acgccggaaa
    11941 tacgtctccg cttccgccag gcggtccgac tggaacgcga tctgcccaag cagttgccac
    12001 gcggcggcga actccggcga catctctacg gccttctcca gatgccggat cgtgtcttcg
    12061 atccgctgct cggataacgc acggcgggca ttccggtact cgcggcgggc ctttggcgga
    12121 atttggagcg cccaaagggg aaccgtgccg gtgcgctttg catattgcac aatggaggtg
    12181 tcccccgccg gtactttgaa ggcgacgtcc atgtggacga caccttttga atcggccttg
    12241 gccgcatcca gttgtacgct cttgcggacc agtccttctc cacgaacaaa aaccgagagc
    12301 gtgtaggccc ctgacgccag tttagtgaag cggaaccgcc cagccttatc gcaggtgacg
    12361 gtcgcatcga acggaaattg ctgggcactc agattggcca gcatcttcgg atacgaaggc
    12421 tgaacctgtc cgcgtaattc aagcttcgcc gcgtttgagc cggacgccgc cagcgcaagc
    12481 gccacaacgg ccgcgaccat actccaattc tatgcccggg acgccgcgca cggtttagca
    12541 cgggggcggt aagcacaccg gaacccggca catacgatat cgttgaattg taaattgccg
    12601 ggccgggcat accgaccctt ccggactcca tgaccccttc tattctgcga gacagtcccg
    12661 acgcgaaatg gcggttgctc gaactcgtgc tccaggccga gacgcacgcc ggcaagccga
    12721 gggagcagtg gccggaaatc attctcatcg gcgatgcgtt cgctcccggt acgctcgccg
    12781 cggcccgcgc actggcgggc gcctacgaag tccagattcg attcgccgag tctcttagcg
    12841 gggcaccgcg gttccatgaa gctcccgacc tatcggcaat gggacatttc ttctcgcgcg
    12901 gcggcttgcc ccaccggcgg atatttttca ccggtgggca tctgcgctgg tcggctcggg
    12961 cacggcgcag gtttgcctac gtccgccagt ctggacgttt tccggatgat ttcgacttct
    13021 ggcgcaagag gttgagcgat ccgaactcgg tccggccgag cacgcgagag aaccgcattc
    13081 aattccgcct ccacacgcag caggattttg accgctttga agaggctgtc cggcagcttg
    13141 aaacggcgaa ttggaccggc ctggctgcag accctgacga agatgccggg tgaaccgtgc
    13201 gcggttcagc agcgctcaaa agcgacagcc gcggcgccca acacaccgcc gtgatagccc
    13261 aaaccggaag cacatacttc aagatggcgc tggtcccaga cggtcaatct gcgcgagagt
    13321 tcttcgcgca cacgcgtcaa tagcagcgtg ttgttctgga ccagtccacc ggaaaggaca
    13381 agcaactcgg gatcgagcaa ctgcactacg gcggccagac cgctggcgag atagacggcc
    13441 tgagcggata tcgcggcacg ggcgctctca tcgcctccgt tggcggcgtg gatcaactgc
    13501 tccgcgttgg cgaactgcgg cgcgcggcgc agcagagcgg cggcgttcgt gtaggcttcc
    13561 aggcagccac gcaatccaca agtgcaggga gcgccatcgg gttcgacggg catatgcccg
    13621 atagcgttgg cgaagaaatg agagccgtgg ttcagcttgc catcgatgta acagccgccg
    13681 cccagacccg ttccaagcgt gatgacgacg aagtttcgcg cggagcgcgc ggcgccgaaa
    13741 cgaaactcgg ccacggcgag cgcattcgcg tcgttctcca cagcaaccgg aagccccacg
    13801 gcttcggata cttcggcagc gatttgcgcg cccgtccaac ccggcaggtt cccagtggca
    13861 tacaccactt ctccgctcga aggatccacc caacccgcgg tggcgatgcc gatagccgcg
    13921 gcgccgcctc cctcgacgca ttcccgcgcg atgcgcttga gatggccgag caacgcttcg
    13981 cggccggcgg accaaggtgt gggcgccacg ccttcgctga gcaggcgtcc atcgctcgcc
    14041 accacgccat acttcgtgtt ggtgccgcct aaatcgatgc cggccacctg ccctgtcacg
    14101 agccggcggc gttcacgatc catagctccg gcgaacacct cgacaatgcg ccgcggcgcc
    14161 gtgatcgccg tgcccacgat gaccgcgtgc gcgcccgcac gcagcgcgga tgccgcttct
    14221 tcgggcgtcg aaatgcgccc ttcggcgagg accggcacag gaacggaatg aacgagctcg
    14281 gctataaatt cgggttcaaa ccgatccacg tgcgcggttt cgtcggtgta gccgcgcatg
    14341 gtggaggcaa cggtgtctgc tccagcttcc actgcccgaa tcgcctcttc aacggtggcg
    14401 atgtcggcca tcacgggaac gcgcaaccgg tcgcgaattt ccttcaggcg ttcgagcgcg
    14461 ccgtaccgcc gtccccgaac ggtgcaatcg atggccacga tggacgcgcc cgcttccaca
    14521 accgcttgcg ccgactcgac ggagggcgta atcaggatac gcccgtcgga cgcgatgctc
    14581 ttctgaatgc cgaaaatcgg cagatccacc ctggctcgta tagcgcgaat atcttccgga
    14641 ccattggcgc gtatgccggc cgcgccgccg agcgccgccg cttcggcaaa gcgggccatg
    14701 gagtccggat cgcgaaacgg atcgccgtcc ggagcctggc aggaaacaat gagccggtgt
    14761 ttgagagaag aaaaaatcat gagagcgtcg tggaagcagc cgtgatcaca atggcaatga
    14821 gaagagtgac aattccgaac tgcatggtgc gcacagcggc aagaggcgca gtgcgccatt
    14881 cgcccagcag atagcccatc aggttcgcca ccagcaatcc aacggcgagg ctgagcggcc
    14941 agccgatgga tggcccgatg tcgcccagca gcggtgtggc cgctccgtat agaaaaatac
    15001 tgccgcccca cagcaggccc atcagaagac ccaaccccca cgtcttcgcc ggcggaccgg
    15061 cgttaaacaa ttttgcgctg cgatgccggg agagcaactg caggcagtag gcgatattcg
    15121 gaacggcgcc cgctcccagc atcagcagcc agatgacgtt cgtcgaagtg aagcgcgtga
    15181 gccccaacct ttcccccgca tcggcaaccg gcagcgccag actgtagccg atgttaaaca
    15241 ccgccgacat cactcccgag ccgatggcaa aaaaataacc gcgccattcc cgcgaggtct
    15301 gctgctccga catgcggccc gcgcgcccac acagccagac gcccgcgatg aacaccgcga
    15361 cgcccgcgaa caggacgaaa acccgcggct ccgctttgag gccgccggtc acgatcaacg
    15421 ggattaccga acccagcgcc gagctgagtc cgatgacgag actattagct aatgagacgc
    15481 ccagcgtgtg tacgctcaaa ccgaaaagga tggcgccgaa cccccacgca aagccgaagg
    15541 tgagagcggc ccaaaccgcc gcggtgggcg cggctgcaat cacggcagac gggtccgccg
    15601 ccggtagcac gacgatggga ggcatcagca cacacgcggt tacgatgaag acgagccaga
    15661 tgttttccca tttccagact ggaatcacct tcattggcgc ggagaacagg ccattcgcga
    15721 tgccggaaag agttgcgtag aacacgccga gaccgatcac ggaagttgct tcctcccttg
    15781 tagaggcact ctgtcattgt atagagggcg acagggttac aatgccgccc ggtggaacgc
    15841 ttcccgtgaa gaccgcgaaa tctcccgaaa ccggggcacg ctcgaaaccg ggtgtcgcgg
    15901 cgcgaaactc gcggcccgcc gcatcgacgt gcaattcaat aggcgagccg ggaacagccg
    15961 agaccttcaa cacgagcttg ccgccggaga ggcttgcctc gacgccgtcc gctccgctga
    16021 aatagacgag ccgggggcct ttgcgctcgg gctcgatgaa gagaatcagc ggatcgtccc
    16081 cttccagggt gacaggaatc tgcgccagtt ccggcgtggt gtaggcgcga tcggacaagt
    16141 agcgattgcg gcgaaggtcc gcaatgcggt aacggctgcc gcgttcgagc ccaagctttt
    16201 ccggcgcaag aattttcagc ggttcgctgc gctttcccga tcctccaaga acgcccatca
    16261 caatgagggc gcggccatcg cgtgcgaagc cgttgacaat cgatccgggc tgcgacatgg
    16321 cgagcacgtc cgcggtgcca aagctcgaat acttctccgc tccgttgagc ccgaagaagc
    16381 gccagtaacc ccacaattcc cgcgcgagcg cgacttccgg ttctttggat acgcgccact
    16441 cctccaccgt cttcggcgca atcgcgcgat tcagacgaaa gggaatcata aacgtcgtca
    16501 gcaggttgat catggttcgc gtgtcctggt tcgggcgcca catggcaagc gactgcacgc
    16561 cttgtcccca gccgttccag gtggtgacga tggtttcttc ccgcatcttg atgggatcat
    16621 cgttttcgcc gttcaggcga atatcgaacc acggcaccat gccggaaaac agcagcatgt
    16681 tggcgttgag cagcatcact ccttttcccg ccgtgtgcct tgccaccacg cggcgcgcgc
    16741 gcttcgcgaa atcgtgatag ccttccgtgg cgaagctgcc cagataaccg ccgcagccgt
    16801 ggcgggcgtt gttgcagaaa cggatgttga tccaatggtc gatgtagagg ccatcgaaat
    16861 cgaaatccgc cagcagttgg tcccattgtt tttccaggtg gttgcgccag ccttcggcgt
    16921 tgtagcacat cagagtagtc tcgttggcat attcaatgcc cgccgaggcc atccattccg
    16981 ccgcgtgttg ctgataggcg ggggccgcga aattgaagtc ggtgaacgtg acgtagggga
    17041 tcacctttat gccgtactga tgggcggttc gaaggaagtg cctgatcttt tcggcttcga
    17101 gaggccgggc gtaatcgccg gaccagtagt tcgctccgcc gatgatcagg ttgacgccca
    17161 tctgggccca caacttcacc tgttcatccg ttggcggctg ccacggttcc cgtgtaccgc
    17221 gccagcgcgc catgacgatc tggtgcggtc cgggccagac ggcacgaatg ctgttcaggt
    17281 cattccgggg atgcttcgcg ggagtcgtct gcacccagaa aacgcgcttg cgaacgtcgc
    17341 ccggattgag gggatacgtg gcgttgcgaa tatcgaactc ctccagttcg agcgcatcgg
    17401 cgcgcctgta gagttgggta aacacgttca cccggccgcg tgaatcccat cccctcaatt
    17461 cccgcccgtc cacggtttcc accgcgccgg tgtcgacccg ctttccgctt tcgagaatga
    17521 cactcgaatc gcgatagccg ctccagcggt ccgcgaagtc ctgcttggag aactcgattc
    17581 cggtgacgcg gttcgcaaga tggaaaaacg cgttccaatg cgactcgaac acgggttgat
    17641 cccgctcagc agcggcgatg gagacggtcg gctccggacg cacgtcgatg ggcggagatt
    17701 tcggatcgct gaccttggtc aggtattgga tgggctcctt ggcgagaaaa gcgtggttga
    17761 agcagttcca gcgaagctgc accggccggg atgtcgtcag gctcacggtg accttcgtga
    17821 aaccatccac atcgaagaga taatcgattt gccaggaaag cggcgtgctg ccgtaagagc
    17881 ctgtggcccg cacctcgacg agctctgcgt tcgctcgcac cagttccgtg cgtatgcctg
    17941 tgcccttggc ggcatcgaag cggaaggcat cggtaagatc gccagaccac aatcgcgggc
    18001 gcatctcgac ggatttttcg tagccgaagc gcggccggtc cacaccacct tcgcgccgcg
    18061 cacccgggtc gtcgtcgggc atcacgccga tatagggatt cggaaaggac gttccggcgg
    18121 cgacaaactc gtcgttcaac gctttggccg acacgatctg gccgatggaa cgatcgacaa
    18181 cataccggaa ggttccgcct gtgatggtga tcgtcttcgg ggtttccgtt acgttcagcg
    18241 gctcgaaaac accgaacact ttgccgtcgt aaagatccgt cttctgcgag tagagagcgg
    18301 aaacagggaa catcgcggcg aatacgataa aaaggactct attcatgatt cagggccttc
    18361 tctatcttct gataatatga gtcgccatgc gcgttatcgg acttgcgatg tgtggagtgt
    18421 tgctggcggg ggtgtgtgcg acggcgcaat tgcggcgcga cgtgccttcc gccgcgggaa
    18481 tgtcggcaga gcggttgaac cgcgcttccg cgctgctggc ggccgaggtg gaatcgggac
    18541 gcgtcggggc ggcatcgctg ctggtggcgc gccgcggacg catcgtattt cataaaggat
    18601 tcggtgtgcc ggcggacgcc gtgtttttgc tggcgtccat taccaagccg gtgacggcgt
    18661 gcgcgctgat gctgctggtg gagcgcgggc aggtggggct gaacgatccg gtgaacctct
    18721 acttcccgga attcaccggc gacgaacgcg aaaagattcg cgttcgggac ctgctcagcc
    18781 atacgtcagg attgccggac atgctgccgg aaaatatcga gctgcggcgc tcgcacgctc
    18841 cgttgagcga gttcgtcaac cgggccttga aaacaccgtt gctgtttccg ccgggaaccg
    18901 ggttccgcta tcagagcatg gggacgctgc tggccggaac gatcgtggaa aaagtgacgg
    18961 ggcaaccgct gcgcgagttc gaaaagaaag agatgttcga gccgttgggc atgaaggaca
    19021 gctcgctggg cctcggcggc aggaaggtgg cggacctcgt cccatgcatg acgtcgccga
    19081 acgcgaacaa ggccgatgaa gagcggttcg gcgcgaacag cgattactgg cgcgatatgg
    19141 gacacccgtg gggcggcatg cacagcacta cggccgacct tgcgattctg ctacagacgt
    19201 ttctcgatgg cggcacgtac ggcggcacgc gcctgttcag tcccgcgacc gtcgcccaaa
    19261 tgacccggaa ccagaacgag gggctgaacg atccatgggg gctcggctgg tcgctcgcgc
    19321 catcgcgcgc gtggaacgcg ctgggcgatc ttgtatccgc cagggcattc ggacatgccg
    19381 gcgcgacggg cacggtagca tgggccgacc cggagacgaa actgctgtgc gtaatcctga
    19441 cgaaccgctc ctatgggagc gacaacggac gcctgctgcg gcgggtgtcg aacgccgtgg
    19501 ccgcggcggt ggagtaaagg cggccgcaca gctttcgaaa gcatgagata atctgcccaa
    19561 atgagcttcc atgagaaccg ccgcgctttt ctaaagacag tggccgcact gccgctgctg
    19621 gctcgcgctt caatggcgaa gccacccaaa acgaacgtct acacacgaat cggtgtgcgc
    19681 ccgctgatca acgcacgcgg aacgtggacg tacctgagcg gctcgctcga gctgccggaa
    19741 gtacgggccg cgaaacatga agcctctctg caattcgtgg acatgttcga gttgcagcgc
    19801 ggcgtaggga aacggctggc ggagctgtcc ggcgcggaat ccggcatggt cacctccggc
    19861 gccgcgggag cgatggcggc ggccacggcg gcttgcatcg cgggcaccga tccgaagaat
    19921 gtctggcaac tgccggacac taccggcttg aagcacgagg tggtgatgtt cggcggccga
    19981 agcgctttcg acaatgcgat ccggctggcg ggaggcaagc tggttctgac gcgcagcttc
    20041 gaagaactgg aagcggcgat caacgacaac accgccatgg tgtacacaac ggccgacgga
    20101 gagagactgg aaaaggcgct ggcgattacc aagcgccgcg gcgtaccgat gctgctggac
    20161 caggcggcgg gaattccgcc catcgagaac ctttcccggt acgcgacgat gggcgtagac
    20221 ctgtacacct tcagcggcgg gaaggggctg gcgggcccgc agtgttccgg gctgctgctg
    20281 ggccgaaagg acctgatcga agcggcactg gcgaactaca atccctggga aggcgcggtc
    20341 tgccggccga tgaaagtggg caaggaagag atcatgggat gcctggccgc tgtggaggcg
    20401 tggaagaaga gggacctggc atcgctcgat cgcgagtggg gcgaacgggt gaaacgcatc
    20461 gccaagatgg tggagacggt ggccggcgtg acgaccgaca tacagattcc ccagggtgga
    20521 aatcgttatc ccacgctcac cgtgaactgg gacgccgagg catggggatt caccgtgcag
    20581 gattgcgcac tccagcttcg cgacggcgag ccccgcatcg aagctcttac gaacgataat
    20641 ccaagcctgg ttcccgccgt gcgcgagggg tatccgaaag cggatcccaa gcgcagcgcc
    20701 cgtcccgacc ggcttcggat tgtgccttcg acgctgcagc ccggtgaaga cctgattatc
    20761 ggcaagcgat tgcggcagat cctgggttcc gcgcgaaaag ggtagtaagt tttcgcgccg
    20821 gcggcgtcta tgtcctgaag ggtatgaaag acaagaaatc cgttcaggac ccgaagcgca
    20881 aggaccccaa gaagcaaggg gccaaaccgc aattttcaca aaagaagatc aaggttccgg
    20941 gcttggagcg cgaaatgtcg cccaaggccg atcacggcga ggagtcctat gaaggtctgg
    21001 gacggctcaa ggaccgcgcg gcgctcatca ccggcggaga tagcggaatc ggtcgcgcca
    21061 tcgccatcgc ttatgcgcgg gagggcgccg acgttctgat cagctatctg cccgaagaaa
    21121 aggaagacgg ggaggagacc gcacggtggg tggagatggc ggggcggcgg gcggtgaagg
    21181 tcccgggcga tatccggaaa gagaggcact gccaggcgct ggtcgaccgc gcgttcaaag
    21241 aattcgggaa gctggatgtg ctggtcaaca acgccggttt tcagatgagc cacgaccgca
    21301 tcgaagactt cacgaccgag gagttcgacc ggacgttcaa gacgaacgta tacgcgatgt
    21361 tctggctgtc gaaagcggca ttgccgcgaa tgaaagcagg cagcgccatt atcaacaccg
    21421 cgtccattca atccttcgat cccagcccga acctgctcgc ctacgcagca acaaagggaa
    21481 cgattgtgaa cttcacgaag gggctttcga agatggcgat gcagcagggc gtgcgcgtga
    21541 acgcggtagc gccgggcccc gtctggacac ccctgattcc ttcgaccatg ccgaaggaaa
    21601 aggtgaagag cttcggcgaa cacacactat ttgaacgccc cgcccagccg gtggaaattg
    21661 cgccggtctt cgtattcctg gccggcaacg aatcgcgttt cgtgacgggt gaggtgtacg
    21721 gcatcaccgg gggccagact ccgtattgag cggcgggcgg agcttcgcgt agaaggcccg
    21781 cagcctctct acatattcgg gaccggccgt ttcagggatg tcgttgtgcc ctgccccacg
    21841 tacacgccaa aagaatttgg gtccgttggc ggcggcgaaa aggcgttccc cgaactcgaa
    21901 cgcaatcact tcgtcctggt ctccgtggag gacgagcagc ggaacatgga cacgtgtaat
    21961 tgttttgagt gagtcgtaac cggagatcaa caacggaccg atgatgggca gaacgcgcgc
    22021 ggcaacggcg cgggcggagg taaacggcgc ttcgagcacg agccccgccg gcgggcggac
    22081 agcagcaaga gatacggcga cggccgtacc cagcgactcc ccgtagagca cgatccgctc
    22141 cggcggaatt ccacggctga gaagaaattc gtatcccgcc tgcgcgtcgc gaaggagccc
    22201 ggcttcacct ggagcgcccc ggcttttgcc atagccgcga tagtcgagca ggagaagaga
    22261 agaaccggca gacgtgatcg ccgggacgcg gtgcgaacgg tgtgtgatgt tgccgccgtt
    22321 gccatggagg tagagagtcg caaggccgtt tccgggcgct tcgatccacc agccgtgcag
    22381 acgcactccg tccgttgtat cgatccagac atcctccgcg ccgaccctgt ctttctgttc
    22441 ccaccagcca tcccgaaggg gatagtaaat ggatcgatgg gcgagaaaag acaggagcgc
    22501 ccagcaggcg gcgacgatga gcagcaggac aatcgttttc gccaacaccc gaatgtatta
    22561 tcgcgcccgg cggcgcgttc cggtttatga ttcgaacatt ttgcgatgcg cgcgttttgc
    22621 ccgcgcgtag ggaccggtcc cgggctcccg cgcaataggg aatgagagcc ttgggcaggc
    22681 cttcgacgtc gcctccagcg tgatcaggat ttacacttcc cgggatagtc ctgcgcgagg
    22741 ttcggtggtt gcctctcggc cagcgagcaa ctactcgatt ccaaaataaa ccagcggttt
    22801 ggaggagaga ggcgcaggcg gcgtgagggc gttgtaaaaa caagagataa aaattgcgta
    22861 ctggcgtgaa cgaacagacc ggtgtaccta aagccgcacg gtaaaacgtc gatagtagtg
    22921 tgaggctatt atgggaccca ccgagtgctt cccgaatcca gccggcgaca acgaggaaaa
    22981 gccgttcacg cttttgccgc ccgccgatcg cggtgaagtt cttcatgaac agcttcgcta
    23041 tctgatggaa catccacgcg gttgtgccga agactgtgcg gaatgccaac ggcttcgctc
    23101 ggtggcggat ctgctgatgg cagcgttccg gtagggaccg aagtacttct attcgcttgc
    23161 ttacgcgcgc ggctctgtaa ctgcttcctc gcggtcgcgg ctcgaaaacg aatgtgagcc
    23221 gcgaccgcgc tgacaccact tagaatcgca agcgcaatcc caactggacc tggcgcatgt
    23281 cctgcgctgc gcgaatcgta cccaccgccg aggaattcag attcgaagtg ggggcattca
    23341 ggttgaccag gttgaagccg ttggtcaact cggcacggaa ttgcagggcg acgttctcac
    23401 gaatggtgaa gtcgcggaat acggcaaaat ccacgttcct gcggccaggt tcatccagaa
    23461 tattccttcc ggaggtacca tcctggccga gcggggtgac ggcaaacgca gatggatcga
    23521 accacatcgc cgtaacggcg tcacggctcc ggttggggtc gaggcgcggg ttgccgacca
    23581 gattggcccg gtcgccactg tcaccgtcga gattccggtc ggttccattc gtcaccgtga
    23641 acggcgctcc gctgcggaag gtggtgatgg ccgaaatggt ccagccgtcg aacagcaggt
    23701 gggcgtagcg cacatcgtgg aaatagttgg tctgccaaac aatggaagcg acgaagttgt
    23761 gccgcctgtc gttgttggta cgcccgcgct cggcggcgag attgttcatg ttctgcgcgc
    23821 cgccggaggt ggtgtcgttc tgcatgcggg ctccctccag gctcttgctg aacgtgtaga
    23881 acgccttcac ctggaaattt ctggacatgc gcttatcggc agtcaactgc aacccgtgat
    23941 acgcgctgtt catgatggag ttaagctccg tgataaccgc cagggtgcca ggttggatgg
    24001 gacgccggtt gttcacgttc ccggtggtgg ctccggcgcc gtaaacggga taattcaagt
    24061 cccgtgcgaa cggcagtttg tgagagagcg aactgacgta tccggcctcg acgctgagat
    24121 cgctgctcaa ctggcgctga accgagaagt tcatctggta ggtatagggc caccggaaat
    24181 ccaggctggg tcccgcgatg gaagccggaa agatgaactg aggggccgag gacgtgtagc
    24241 tgtaaggata aggagagaca ccgcccggca acagaccgta aggatcggtc agactcgcca
    24301 cgttattgaa gcgctggcga atggtaaacg gctggttatc ggccgtggag ttccattcgt
    24361 ttccggagat gccgccatag aacaaaccga agccggtgcg aatagaggtc ttactgtcac
    24421 caaacggatc ccaggccagt ccgattcgtg gcgcgaagtt gttcctgtcg gatgagatga
    24481 tgccccggcc aatgccttca tctccgggga agagcagtcc cacaggagcg ttgggaacaa
    24541 cggtggactg ccgcccctga atgaatgtca gcttgcgatc atgcgggtcg gtggcgggtg
    24601 tctggaaatc gtaccgcagc ccgaggttga gcgtcagccg cgggtgaatg cgccagtcgt
    24661 cctgaatgaa cagaccggtg taccaaacgt tgtcgatctt ggttaccggg gcatcctggt
    24721 tcatggtgac agggcggccc agcaggaaat cggccagcgc gtttttgctg atggtgccgt
    24781 tgaaggagaa ggtgccgtag ttgttcagcg tggtgtcgtg gataaacttc tccaggtaaa
    24841 cctcgccgcc gaacttcagc gtgtggttgc cccgattgag gctgaagttg tcccggaggc
    24901 cgtagtaatt gctcccggcg ctgggtccgg caatggcctg cgtgagattg aagtagccgg
    24961 agacctgaat ctgcggaaga gacggcgcac cctgaatctg aaacccggac ccgagatcgc
    25021 ccaaagagat ttccggaatg ttcaggcgtc cgccgaagtt acggacatag gtgagccgga
    25081 gttggttgac ggcagtgggg ccgacggtcc aggtatcact cacattgaaa ttctgctgtg
    25141 tccacttgaa ctgccgctgc gaccagatga gattgccggc aagcgattcc aggctggtac
    25201 cggtgtcgcg gaaatagctg cccgtcaact ggtgcgactg agagagcata tggtcgacct
    25261 tgaactgcag ctcgtcggta tcataaggct ccggttgcgt gacctcgacg aagttgcccg
    25321 gcaggttggc ctggggaatg tactcgtcga ggatccgctt cgcggtggga tcgaaccggt
    25381 tggacggaat cacgccgccg ggaaagggct ggcctgtcgc cgggtccacc ggctggatgg
    25441 cggattgcga gaaattgccg gcgcgttcga gagacgtggg aacgatggcg gtgttcttaa
    25501 actcgtactc ccgctgacgc agcccggagt agctgccgaa aaagaaggtt ctgtcccgca
    25561 gaaccggtcc acccagcgtg ccaccgaact gattgcggcg gagcggagct ttgctactgg
    25621 cgttccaggt ggtggcattc agcttgtcgt tgcggatgaa ctcgaacaaa gacccgtgga
    25681 attcgttcgt tccggatttc gtcacaacgt cgatgacgcc gccggcaaag cggccgaact
    25741 cagcgccgta gctgttggtg atgacgcgaa actcctggac ggcgtcggga ttcggtacgg
    25801 cgtttccggt tccccgaagg ccggtggtgt tgttgccccc gtcgaggaag taattcaccg
    25861 taccggaccc accgtaagaa ccgccattca ccatggtgcg ctgttcgcgg aacccaaagg
    25921 cgttcccgct ctcggaagct tccactccgg gcgtgaggtt gagcagcgaa tagacattgc
    25981 ggttcaccag cggaaggttg atgatttccg tgttctcgac ggtacgtccg accacggccg
    26041 aggtggtatt gaccagcggc gcatccgcgg tgactgaaac gacttcggta agcgctccaa
    26101 tatcgagtac cggatcgacg cgcgccgagc ggccgacgtc tagaacgaca ccggtctgca
    26161 cgaacttctt gaaggtgctg gtgaccactt cgacgctata ggtaccggtt ggcaggaacg
    26221 gaatcgaata ctggcccaac tggtcggtat ccatcgaacg cgagaagttt gtttcgagat
    26281 tcgtaaccgt gacgcgggca ttgggcacca cagcaccgct acggtctgtg actgtgccga
    26341 gaatagttgc tgtgcttacc tgtgcatgga ggtaagaaaa aggaagaatt gccgcaagcg
    26401 cggcgatgca gagcacattt cgcgagtaca cgtgacctcc tcaaaaaatg cggcccgccc
    26461 cgtttcctcg agcagacctt aagacccaag ctagttactc gcagtataca ctgcggcgga
    26521 gtattgtttc aggtacaggt gtagaggtcg cattattttt acaagcgaat cagcgagacg
    26581 aggtactcct gcggcaaacg caacgcgcca tccttcgcct ggtttgcatt tctccagatc
    26641 tcgacgacgt gttgcctcag ttccccgaaa cgatctccga gcatggtccg cgccatgacc
    26701 atgggaccga aacgttccat aaaaaagtct gtccacgcgt ccagcgaatc ccactggatg
    26761 atgtttacat gacgctcgaa ctcgaagccg gccgcagtgc ctgcgaacag ttcgcgcacg
    26821 tgttcctcgc taccccaaag aattggcggc aacgcatagc cgggcggcgg aggcatatag
    26881 gcggcgctca ctttgaaaat gtgcccaacg gtgccctccg gcgtccaggt cgcggtgacg
    26941 atcgcaccgc cgcggcggca gacgcgcgcc atctcctcag cggttctttt gtggcggggc
    27001 gcgaacatat ggccgaaggt agatagaacg cggtcgaagc tcccgtcctc aaagggaaga
    27061 ttctcggcgt caccctcacg ccattccatc tcaagtcctt cggcttcggc tttggacttc
    27121 cccgcctgaa gcagctttgg cacgaagtcg agccccgtgg tccgggcgcc ggcgcgggcc
    27181 gcgatgcgcg cggcattgcc ggtgccacac gcgacatcga gtactttcat ccccggttcg
    27241 attccggcct tggcaacaat ccgttcggcc acgtcagcga tgtacggtga gagcgcgctg
    27301 tattcccctg cttcccagat tgcgcgctgt ttcgtcttga actcgtccag ttgaagaatc
    27361 atacgatccc ctcattggat atgcagctta tcgcttgata atattacccc catccaggta
    27421 tggtgcgcag ctgcatacgg cgcaccaagt cctctgtgaa gggtgcattc acgaaggaat
    27481 tcacttgcgt ttcgacctgt gagcagagcg gctcaggcac gcgcgatgta tccatcgaac
    27541 aagccggggc tcggtgacct tctgtccggt gctgtaggcg aaccacacag aatacacttt
    27601 gcggtgactc ttccatccag tgcgattcaa tcttcgggtc caatgcggcg atgtccgctt
    27661 tgatccgaag catgagatcc gccgtaaacg gagcatttcg atagggcgcc aaacgcgcgt
    27721 ctacctgcga gcggcggggc cggtcaccgg acgctttcag ggctgtagaa acggaagcgc
    27781 gagcattccg aagaggacgg cctgcaacac gatcaactgc gcgcgggcgg aacggactcg
    27841 cagcagagcg ggcatgatga cggcccaccg caacaagaat catcgactgc accgcccatg
    27901 caagcagatt ggcgatcatt ccttgctccc tttcttcttc aacattcgcc tgatttcttc
    27961 aatttcggcc gggctgacct gttcgttccg tacgatgtgg agcagcaggg gtttggccga
    28021 tccgttgaag agagcaaagt ttgtgcaggg ggacttccgc ttccattcgc tacagtgcga
    28081 ctgagatcct gcggttcgcg gccgcctcga atgctcggag gcgcgccttg cgcctcatgt
    28141 ctagcaaaaa ccgctccgga ctttcaggag cggcagtccg gcaagggtgc gatccatcac
    28201 cggtcccttt tcagtttggc ggccgtgcgc aaaaaccgaa cctttccccg gagcgcccga
    28261 agctgcgcgt agttctgcga cgcggcgatc agttggagac cggttcgagc tgttcgtgtg
    28321 ataccggttc cgctggcctg ctgtgccctt tccagaagtt cggccgggac ctcgacggta
    28381 atcttgcgag cgacgctcac gcccggagga taccatcctt cgtaccagca tcgagtatgg
    28441 ggaacaacgt caatccgagg ggcgcccctc gctacctctg atcactcgtg gcgcaaggcg
    28501 gtcaccgggt caatgcgcga ggcgcgccgg gccggtacga acccggcggc cagggcgaca
    28561 gcgctcagca atagagtgca tcccgcgaca acggatccct ccgccggttt cactccgtag
    28621 agcatgctcg aaacgtagtg cgttgccgct acagccccaa cgataccgat cgcactgccc
    28681 acaacaacga gcactagcgt ttctcgaaaa gcgagccact tgatgtggct gggctgagct
    28741 cccaaggcca tgcgtatgcc gagttcaccg gtgcgccgcg acagatggta tgccatcacg
    28801 ccgtaaagtc cgatgcaggc cagaagtgtg gccagggcgc cgaatgcggt cgagagagtg
    28861 gcgatcaatc gttcccggct gaacgtaccc tccactatgg cgggaagctg acggatatgc
    28921 tccacttgcg cagtgctgcc ggctgcggag accgcctcgc ggatatcgga gctaagcagg
    28981 ctggtatgct catggacgcg gaccaaaaaa ctgccttcct tcgcgcccgg cagataagtc
    29041 atgtcccacg tatgctcgcg cgcaccgtaa tgcttcgcgt cgcgtactac gcctacaatc
    29101 tcccgaagct ctggcttaag gccccgcagt ctgatgcgg
//
LOCUS       DASTMK010000143         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_8263, whole genome shotgun sequence.
ACCESSION   DASTMK010000143 DASTMK010000000
VERSION     DASTMK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8263"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFQ79_06035"
     CDS             complement(<1..534)
                     /locus_tag="VFQ79_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0139247.1"
                     /translation="MPEEQPGVHELFDIEGRVAVVTGGARNLGYDMALALAEAGADVA
                     VTSRTLQAAEASAARIAARTGRRISGFLCDVAEEASVESMVNAVLEAFGRIDILVNNA
                     GNVVSTPENAPLERRPIEEWRHTIDVNLLGVFLCSKHVVAKAMIPARSGVIVNIGSTA
                     GMIGKDRRIYRGTSMGGA"
     gene            complement(551..1447)
                     /locus_tag="VFQ79_06040"
     CDS             complement(551..1447)
                     /locus_tag="VFQ79_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0139248.1"
                     /translation="MNVNRGILPALVTPFDADGVFAARSFERLLERVYAADVDGVYVC
                     GQTGEGLLQPAAQRKCVAEVAVRNSPPGKTVIVNIGAYTTADAVDLARHAARAGVHAI
                     SSLPPAGNYSFDEIRAYYQAIASASDLPLLVYYFPELCPALANTGQLLELCSIPNVIG
                     LKFTDFDLYKLSLLKREGAVVFNGRDEVLAAGLLMGADGGIGSFYNLVPDLFVTLFRH
                     SEAGEWEQAREAQQRINELISISLRFPVFPAIKVMLAWSGIPCGTCLPPRRPLTPAEE
                     SCLQEMLGESSLSDRAFSFAAR"
     gene            complement(1498..2199)
                     /locus_tag="VFQ79_06045"
     CDS             complement(1498..2199)
                     /locus_tag="VFQ79_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0139249.1"
                     /translation="MKITFRSTRKPGPLKTPVADSIRRAIYAGKLQPNDALRELHLAR
                     ELKVSQATVREALVQLEQEGLVVRVPNKETTVTNFTREEVRERLELRIVLEGIAFVEA
                     AARMDGKHHAELSRLAAAIEDALRNKGPHELAQADLRFHRYVWEQSGNNTLYRVLVNL
                     SAPLFAFLGILRRKGVEQPVPDVQPHEALIAALRTRNPQQIEEAVREHIRGSTYTNFL
                     GSGVSDFKQYAQKAG"
     gene            2328..>4558
                     /locus_tag="VFQ79_06050"
     CDS             2328..>4558
                     /locus_tag="VFQ79_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEU0139250.1"
                     /translation="MSSNRRTGRFLALLAVLCTNVFAEAAPEILRWTRLPELPRAIAG
                     GFAGEANGALLVGGGTYFENSGSGNGAVVPSDLVFVLTPGGAGWKIRRLERPRAFAAA
                     VPSNRGLILIGGTDPKGPSATVNSIYWDGDRLKVEVLPHLPAPLGHSRAALLDGVIYL
                     AGGQRSTAAAPVREFRALRLDGTGVGWQELEPWPGPARISPIMAAADGSVYLFGGIGS
                     TAESESDRHLRDAWRYRPGVGWTRLPDLPRPVADAPALAYGQSHILVFGGRAAGGDRD
                     VWAFHTLTETWARMGTLPESVPATAAVHWQGQVVIPGGTSHANQPSSRVLAATAAESR
                     QSFGILDYMVLALYLAGIVLMGVYLSRRNRNTGDFFLGGRRVPWWAAGLSIYGTQLSS
                     ITFMSIPAKAYATDWTYALVNLCVLLVAPVIVFFYMPFFHRLRVTTAYEYLEKRFNRA
                     VRLLSSALFLAFQLGRVAVVMFLPAIALATVSDTNLYACILAMGGMAIVYTVLGGMQA
                     VIWTDVVQSFVLLGGALLSLYLAVSAAGSDLAGLFSIANDAGKFHIFDWRWDWTIATV
                     WVILIGNGASVLATYTTDQTVVQKYLTTADERKSASSIWTNALLTIPSTLIFFGMGTA
                     LFVYYRAHPAELNPHLPTDAILPWFMVQALPAGIAGLVLAGLFAAAQSTLSSSMHSMA
                     TVVMVDFYRPFYPETNERRELHLARLLTLLLGVLGTGLALLLATIDIRSLWDVFLTLL
                     SLLG"
BASE COUNT          898 a         1349 c         1333 g          978 t
ORIGIN      
        1 tgctcctccc atcgaggtgc cccggtaaat gcggcggtcc ttaccgatca tgccggcggt
       61 ggagccgata ttaacaatca cgccggaacg cgcgggaatc atggctttgg cgacgacgtg
      121 tttggaacaa agaaacacgc ccaacaggtt cacgtcgatc gtgtgtcgcc attcctcgat
      181 cggccgtctt tcgagaggcg cgttctcagg tgtgctgacg acgttccccg cgttgttcac
      241 gagaatgtcg atgcggccga atgcttccag tacagcgtta accatacttt cgaccgatgc
      301 ctcttccgcg acgtcgcaaa gaaaacccga gattctgcgt ccggttctgg cggcgatgcg
      361 agcggcggac gcctcggccg cctggagcgt ccggctcgtc acagcgacgt ccgcacccgc
      421 ttcggctaaa gccaaagcca tgtcgtagcc caagttccgg gcgcctcccg tgacgacggc
      481 aacgcgccct tcgatatcga acaactcgtg aacgccaggc tgctcctccg gcatgagttt
      541 cgcctccgtt ttaacgggcc gcaaaactga aggcgcggtc cgacaaactc gattcgccga
      601 gcatctcctg aaggcaggat tcttccgcgg gcgtcaaagg gcgccgcggc ggcaggcacg
      661 ttccgcaagg aattcccgac cacgcaagca tcactttaat ggcagggaat acgggaaagc
      721 gaaggctgat ggagatcagc tcgttgatcc tctgctgcgc ctcgcgagcc tgttcccact
      781 cgcctgcctc actgtgtcga aacaacgtca caaagaggtc cggaacgaga ttatagaagc
      841 tgccaatgcc gccatcggcg cccatcagca ggcccgcggc aagaacctcg tctcgcccgt
      901 tgaagacgac ggccccctcc cgtttgagaa gcgacagttt gtagagatcg aaatccgtga
      961 acttcagccc gattacattc ggaatggagc aaagctccag cagttgtccg gtattcgcga
     1021 gggcaggaca aagttccgga aagtagtaga cgagaagtgg aaggtccgaa gccgacgcaa
     1081 ttgcctggta gtaagcccgg atttcatcaa atgaataatt accggccggc ggaaggctgc
     1141 tgatggcgtg aacccccgcg cgcgcggcgt gccgtgccag atcaaccgcg tccgcggtag
     1201 tgtaggcgcc aatatttaca atgacggttt tgccgggagg cgagttccgg actgctactt
     1261 ccgccacgca cttccgttgc gcggccggtt gcaggagacc ctcaccggtc tggccacaga
     1321 cgtaaactcc gtccacatcg gcggcgtaaa ctcgttcgag cagccgctcg aacgatctgg
     1381 cggcaaacac accatccgca tcgaaaggtg tcaccagggc aggcagtatt cctctattta
     1441 cgttcatagg caattagcgg tcctatctcc acaaagtatc gatttcgccg cccaacctta
     1501 gccggctttc tgcgcgtact gcttaaaatc actcacaccg gaaccaagga agtttgtata
     1561 ggtcgaaccc cggatgtgtt cgcggacggc ctcttcaatc tgttgcgggt tgcgagtgcg
     1621 aagggccgct atcagcgctt cgtgcggctg aacatccgga accggctgct cgacaccttt
     1681 gcgacgcagg atgcccagga atgcgaagag cggagcgctc aaattcacca gcacacggta
     1741 caaggtgtta ttgccggact gttcccacac gtaccggtga aaacggagat ccgcctgtgc
     1801 gagttcatgc ggccccttgt ttctaagggc gtcctcgatg gccgcagcga gccgggacaa
     1861 ttccgcgtgg tgttttccgt ccatacgagc cgcagcctca acgaaagcaa tgccctcgag
     1921 cacaatgcgc agttcgagcc gttcccgcac ttcctcgcgg gtgaaattcg tgacggtcgt
     1981 ttctttgttc ggcacccgca caacaagacc ctcctgctcc agttggacca gggcttctct
     2041 gacggtcgcc tgactgactt tcagttcgcg ggcgagatgc agttcgcgca aagcgtcgtt
     2101 tggctgaagc ttgcctgcgt agatcgcgcg gcggatcgaa tcggcgacag gcgtctttag
     2161 cggaccgggt ttcctcgtcg agcgaaacgt gattttcata ggtaccgata tcgataatag
     2221 tgtgtttccc ggcattccgt caatcaaata tagaaatcta aatcctcatc aacaactact
     2281 ggcacgattt ggcaaaagtg cgaagatcat ctattatcga tatcgttatg tcatccaata
     2341 ggcggacggg gcggtttcta gcgctgctgg cagtcctctg cacaaacgtg ttcgcagagg
     2401 ctgcaccgga aattctgcgg tggaccagat tgccggagtt accgcgcgcg atcgcaggag
     2461 ggttcgccgg agaagccaac ggtgcgttgc tagtgggcgg cggaacttat ttcgagaact
     2521 ccggttccgg caatggcgcc gtagtaccca gtgatctcgt attcgtactc acgcccggcg
     2581 gagcaggctg gaagattcga aggctcgaac gcccccgggc gttcgccgca gcggtcccct
     2641 cgaaccgcgg gttgattctg attggcggca cagacccgaa gggaccttca gccaccgtga
     2701 attccatata ctgggacggc gatcgtctca aagtcgaagt cttgccccac cttcctgccc
     2761 cgctaggcca cagccgcgca gctctgttgg acggcgtgat atatctggcc gggggacagc
     2821 ggtccacggc ggccgcgcct gtccgtgaat tccgggctct gcgtctggac ggtacgggtg
     2881 tgggttggca ggaacttgag ccttggccgg ggcccgcccg aatctcgcca attatggcgg
     2941 cggcagatgg atccgtttat ctgttcggcg gtatcggaag cacggcggag tccgagagcg
     3001 atcggcatct tcgcgacgca tggcgctatc gccctggggt aggctggacg cggttaccgg
     3061 acttgccccg gccggtcgcc gatgctcccg cgcttgccta cgggcagtcg catatcctcg
     3121 tgtttggcgg gcgggccgcc ggcggcgacc gggacgtatg ggcgtttcat acactcacgg
     3181 aaacgtgggc ccgcatggga acccttccgg aaagtgtgcc ggcaacggcc gctgtgcact
     3241 ggcaggggca ggtggtgatt ccgggcggca catctcacgc aaatcagccc tccagcagag
     3301 tgttagccgc gacagcagcc gagtcgcggc agtcgttcgg aattctggac tatatggtgc
     3361 ttgcacttta tcttgcgggc atcgtgttga tgggcgttta cctgagcagg cgcaaccgga
     3421 acaccgggga tttttttctt gggggaagac gagtgccgtg gtgggccgcc ggcctgagca
     3481 tctatggcac gcagttgagt tcaatcacct tcatgtcgat ccccgcgaaa gcctatgcca
     3541 ccgattggac gtacgcactc gtaaacctgt gcgtactgct ggtagcgccc gttattgttt
     3601 tcttttacat gccgtttttc caccgcctgc gtgtcacgac ggcctacgaa tacctggaga
     3661 agcgattcaa ccgggcagtg agacttctct cgagcgccct gttcctcgct tttcaactcg
     3721 gacgcgtggc tgtcgtgatg tttctgcccg ctatcgccct cgcgacagtc agtgatacca
     3781 acttatacgc ctgcattctg gcgatgggtg gaatggctat cgtatacacc gtgcttggcg
     3841 gaatgcaggc ggtaatctgg accgatgtcg tgcaatcatt cgtactgctg ggtggcgccc
     3901 tgctcagttt gtacctggca gtatcggcag caggttccga tctggcaggc ctgttttcca
     3961 tcgcaaatga tgcgggcaag ttccacatct tcgactggcg ctgggactgg accatagcaa
     4021 cggtttgggt gatcctgatc ggcaacggag cttcggttct cgccacgtat acaacggatc
     4081 aaacagtagt tcagaaatat ctgactaccg cggacgagcg caaatcggcg agttcgatct
     4141 ggacgaacgc gctactgacg ataccatcca ccctcatctt cttcggaatg ggcacggcgc
     4201 tcttcgttta ttacagagcc catcccgccg aactcaaccc gcatttgcct acggatgcga
     4261 tactgccctg gttcatggta caggcactgc ctgctggaat cgcgggcctg gtgctcgccg
     4321 ggctctttgc ggccgctcag tcgacgctat cgagcagcat gcacagcatg gccacggtgg
     4381 tgatggtgga cttttaccgt cctttctatc ctgagacgaa cgaacggcgc gaactccatc
     4441 ttgcgagact cctcacactt ctgcttggcg ttttgggcac agggctcgca ctgctgctcg
     4501 ccaccatcga tataaggtca ctttgggatg tgttcctgac cctgctctca cttttagg
//
LOCUS       DASTMK010000144         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_231477, whole genome shotgun sequence.
ACCESSION   DASTMK010000144 DASTMK010000000
VERSION     DASTMK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231477"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1339)
                     /locus_tag="VFQ79_06055"
     CDS             complement(164..1339)
                     /locus_tag="VFQ79_06055"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU0139251.1"
                     /translation="MTGKLALVLALLLAAAGCGSSRTDSPVQAVSAAGGSPLLIDTHN
                     DVTSDTVKGLDIGPRRTEGHTDIPRLREGRTGAVFFAAYVAADYAKKGKAAYRALEMI
                     DTIRHDIVARHPDTFVLATTADEIEAAHRDGKIAALIGIEGGHAIENSLRLLRQFHAL
                     GVRYMTLTHSNTNDWADSSGDMDNPGVKHHGGLTVFGREVVAEMNRLGMMVDISHVAD
                     KTFWDVLEISKAPPFASHSSCRALSNIPRNMTDEMIVALAKKGGTIQINVGCEFLSQV
                     SADASSWTNKEIAARLRRLPPEERRKAAREIDAKMPRPTLADVVTHIDHVVKIAGIDA
                     VGIGTDFDGVSCVPVGFEDVSRFPALEKALLEKGYSAAQIGKIFGGNILRVMRNAEQ"
     gene            complement(1336..2313)
                     /locus_tag="VFQ79_06060"
     CDS             complement(1336..2313)
                     /locus_tag="VFQ79_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0139252.1"
                     /translation="MKLCTFEVRTPLGRQRRAGAVLDGGILDLNFGCAALLARRGEPL
                     PHRLADALVPAAMLEFLDVWSRSFPHALEVIASVEDGPGPNGETLLYRPEEVRVLAPL
                     PNPRSLRDFYAFEGHVKKGFEKRGEPMPQEWYEMPVYYKSGHHNIIGSGEDVLWPSFT
                     EKFDYELELAAVIGKQGRNIPVERAREHIAGFTVMNDFSARDIQRKEMKVRLGPAKGK
                     DWATALGPWLVTADELPNPYELRMTARVNGELWSNGNSGSLFWRFEQMIEFLSRDDTI
                     YPGDVLGSGTVATGCGLELDRWVQPGDVIELEIEKIGVLRNRVVRHGKS"
     gene            complement(2332..3462)
                     /gene="hppD"
                     /locus_tag="VFQ79_06065"
     CDS             complement(2332..3462)
                     /gene="hppD"
                     /locus_tag="VFQ79_06065"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEU0139253.1"
                     /translation="MPQSLENRNTSPGTDFLPLNGIDHLEFFTGNAKQAAHYYRTAFG
                     MTLSAYCGPETGVRDKVSYVLESGKIRFVLTTALGPDHPAARHALLHGDGVHSIALEV
                     EDADAAYRETVARGAAGVTEPYTLRDASGEVRLAVIAAYGDTVHTFVERARYGGAFLP
                     GFLPVQSTDRVARPAGLRYIDHIVGNVGWGEMNRWARYYEEVLGFRLYQTFDDKDIST
                     EYSALMSKVMANGNGRIKFPINEPAEGRRRSQIEEYLNFYGGPGVQHIAMGTADIIDT
                     VTKMRDQGVEFLHIPTAYYNDLVSRVGAIDEPIDRLASLGILVDRDDEGYMLQIFTKP
                     VQDRPTLFYEVIQRKGSRSFGKGNFKALFEAIEREQARRGNL"
     gene            complement(3419..4540)
                     /gene="hisC"
                     /locus_tag="VFQ79_06070"
     CDS             complement(3419..4540)
                     /gene="hisC"
                     /locus_tag="VFQ79_06070"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0139254.1"
                     /translation="MPLVPPYIKNLRPYEPGRNAEEVRKQFHLAHVVKMASNENPLGP
                     SPLATDAIATCLPRLNLYPDGGLGLRRELASRYDLKVENVIAGSGSEGIMSNIIRTFL
                     CDEDEVLTTESAFIGFQVLARSRGVRYRTVPYREWHYDLDALAAAVNDRTKIIYLANP
                     NNPTGTIFTRQEFDRFYRHIPERVLIILDEAYFEYAKDNPRYPDSMHYRYDNVITLRT
                     FSKIYGLAGLRIGYGFAHEQLIANLLKVKLPFEPSTPAQTAGLAALSDREFLHRSLEL
                     NARGLRYLAKNLGELGLEVVPPEANFLMIALASAEQAGALSLDLLQQGIIVRQLGAFG
                     LPRCIRISTGTDDENELCVQALRRSYAAISGKPEYQSRH"
     gene            4684..5403
                     /locus_tag="VFQ79_06075"
     CDS             4684..5403
                     /locus_tag="VFQ79_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEU0139255.1"
                     /translation="MNMAALDASERKPSMVDRIIGMFNIFLDPAGAAKNIRLSWSWVA
                     PLVLSSIVIGICGYLLIPTTLRIMQMNPPPNMTAEQFEKARPIIEMSQKVSALASPVI
                     IGVFTALFAGILTAACAVLDIRSRFRDHFSLLAHTGLIGALGYLCGFLVIRLRGDAVQ
                     SLLELRPGFGLDLFLGEHANKFVYGLLNFFSLFQLWNIVMLGLTFAFLTNVSKGRAFA
                     ATAPVWLLGLVLALVGVMFQR"
     gene            5426..>5671
                     /locus_tag="VFQ79_06080"
     CDS             5426..>5671
                     /locus_tag="VFQ79_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139256.1"
                     /translation="MNAGKIEEVTEAALPAVPLTVEGSSVLHQMLRFRWAQWKALAPG
                     RRREIAAEAASALEQMEQPGRKQSAVYSLLGHKGDLML"
BASE COUNT         1095 a         1641 c         1722 g         1213 t
ORIGIN      
        1 ggggtatgct ttgcgcgtgc atcgatcacc tgatgaaccg gtattaccag aagcgccggg
       61 gaggaggaga cggctgctgc tgctgaaaca aactcccgcc ttgtcatttc cgctcctgag
      121 taaccgcttc ctggcggtag cggctcggta acctaattga attttactgc tccgcgttgc
      181 gcatgactcg cagaatgttt ccaccgaaga ttttgccgat ctgcgcggcg gaatatccct
      241 tctcgaggag cgccttttcg agagcaggaa atcgggagac gtcctcgaac ccgacaggaa
      301 cacaactgac accatcgaag tccgtaccga tgcccaccgc gtcgatgccc gctattttca
      361 cgacgtgatc gatgtgtgtg acaacgtcgg ctaacgtggg gcgcggcatc tttgcatcga
      421 tctccctggc ggctttcctg cgctcttcgg gcggaaggcg gcggagacgg gccgcaattt
      481 ccttgttcgt ccaggaggaa gcatcagccg agacttgtga caggaattcg catcccacgt
      541 tgatctgaat ggttccgcct ttctttgcca gggccacgat catttcgtcg gtcatattcc
      601 ggggaatgtt ggatagcgcg cggcaggaag agtgcgaagc aaagggcggc gccttcgata
      661 tctccagcac atcccaaaag gttttgtcgg cgacatgcga gatgtccacc atcattccca
      721 gccggttcat ttccgccacc acttcgcgtc cgaagaccgt gagcccgccg tggtgcttca
      781 caccgggatt gtccatgtct cccgaggaat cggcccaatc attcgtgttc gagtgggtca
      841 gtgtcatgta gcggacaccg agcgcgtgga actgccgcag cagccgcaaa ctgttttcga
      901 tggcgtgtcc gccctcgatt ccgatgagag cggcgatctt gccgtcgcgg tgtgcggctt
      961 cgatctcatc ggccgtcgtt gccaggacga acgtgtccgg gtggcgcgcg acgatatcgt
     1021 gccggatggt gtcgatcatc tcgagtgcac gatacgcggc tttgcctttt ttggcgtagt
     1081 ctgccgcgac ataagccgcg aagaacaccg cgccggtacg gccttctcga agacgtggga
     1141 tgtccgtatg tccttcggtg cggcgcgggc cgatgtccag acctttgacc gtatcgctgg
     1201 tgacgtcgtt gtgcgtgtcg atcaaaagcg gggagccgcc ggcggcggac acggcctgaa
     1261 cggggctgtc tgttcgagag ctgccgcaac cggctgccgc cagaagaaga gccaggacga
     1321 gagcaagttt gccggtcatg atttgccgtg gcgcactacc cgattccgca gcacgccgat
     1381 cttttcgatc tcgagctcta tcacgtcgcc cggttgcacc caacggtcca actccaggcc
     1441 gcagcctgtc gccaccgtac cggagcccag cacatcaccg ggataaatcg tatcgtcgcg
     1501 cgatagaaat tcgatcatct gttcgaatct ccagaatagc gagccggagt ttccattgga
     1561 ccacagctct ccattcaccc gcgccgtcat gcgaagctca tacgggttcg gaagctcgtc
     1621 ggccgtcacc agccacggcc ccagcgccgt cgcccagtcc ttgcccttgg ccgggccgag
     1681 gcgcaccttc atttccttgc gttgaatgtc ccgcgcgctg aagtcgttca tgaccgtgaa
     1741 tccggcgatg tgctcgcggg cgcgctccac cgggatgttg cggccctgct ttccgatgac
     1801 ggcggcaagc tccagttcgt agtcgaattt ttccgtgaac gacggccaca acacgtcttc
     1861 accgcttccg atgatgttat gatggccgct tttgtagtag accggcattt cgtaccactc
     1921 ctgtggcatc ggctcgccac gcttctcgaa accctttttc acgtgtcctt cgaacgcgta
     1981 aaaatcgcgc agcgagcgcg ggttgggcaa tggcgccagc acgcggactt cttccggcct
     2041 gtacaacaga gtctcgccgt tcgggcctgg gccgtcttct accgatgcga tcacttcgag
     2101 cgcgtgcgga aacgacctgg accagacgtc gaggaattcg agcattgccg ccggcaccag
     2161 cgcatccgcc aaacggtgtg gaagcggttc gccgcggcgt gccagcaggg cggcgcagcc
     2221 gaagttaaga tcgaggattc ctccatcgag gaccgcaccg gcacgccgct ggcgtccaag
     2281 cggagttcga acttcgaatg tgcagagttt catcgaactc gaaagaaccc gtcacaagtt
     2341 accacggcgg gcctgctcgc gctcaatcgc ttcgaacagc gctttgaaat tgcctttgcc
     2401 gaagctgcgg ctgccctttc gctggatcac ctcataaaac aatgtcggcc ggtcctgaac
     2461 gggtttggtg aagatctgca gcatatagcc ctcgtcgtcg cggtcgacga gaattccgag
     2521 ggaggcaagc ctgtcgatgg gttcatcgat ggcacctacc cggctgacga ggtcgttgta
     2581 atacgcggtc gggatgtgaa gaaactcgac tccctggtcg cgcatcttgg tgacggtatc
     2641 gataatatcg gcagtgccca tcgcgatgtg ttgaacgccc ggcccgccgt aaaagttcag
     2701 gtactcttca atctgcgagc gccgcctgcc ctcggcgggt tcgttgatcg ggaacttgat
     2761 gcggccattg ccgttcgcca ttaccttgga catcagggcg gagtattcgg tactgatgtc
     2821 cttgtcgtcg aaggtctggt agagccggaa gccgagcacc tcttcgtagt agcgcgccca
     2881 ccggttcatt tcgccccaac cgacgttgcc gacgatgtga tcgatgtagc ggagccccgc
     2941 tggccgggcg acgcgatccg tggactggac cggcaaaaag cccggcagga aggcaccccc
     3001 atagcgcgcc cgttctacaa acgtgtgtac ggtgtcgcca taagcggcga tgaccgcaag
     3061 acgaacttca ccgctcgcgt cgcgaagcgt gtatggttcg gtcacgccgg ccgcgccccg
     3121 cgccaccgtc tcgcgatacg ccgcgtccgc gtcctccact tccagcgcga tgctgtgaac
     3181 gccgtcgccg tgaagcagcg cgtgccgggc cgccgggtgg tccggaccca gcgcagtagt
     3241 caacacgaaa cggatctttc cggactcgag cacatacgat actttgtcgc gaacaccggt
     3301 ttccggaccg caatacgccg aaagcgtcat gccgaacgcg gtccggtaat agtgcgcggc
     3361 ctgtttcgca ttgccggtaa agaattcgag atgatcgatg ccgttgagag gcagaaaatc
     3421 agtgcctgga ctggtattcc ggttttccag agattgcggc ataactcctc ctgagagcct
     3481 ggacgcagag ttcgttctcg tcgtctgtgc cggttgaaat gcgaatacat cgcgggagac
     3541 cgaatgcgcc aagctgccgg acaataattc cttgttgcag caggtctaag ctcagagccc
     3601 cggcctgctc tgcgctggcg agcgcgatca tcaggaagtt cgcttccggc ggaaccacct
     3661 ccaagccgag ctcgcccaga tttttggcca ggtagcggag cccgcgggca ttcaactcca
     3721 gcgaccggtg aagaaattcg cgatcggaca gcgcggcgag gcctgccgtc tgcgcgggcg
     3781 tgctcggttc gaatggcagc ttcactttga gaaggttggc gataagctgt tcatgcgcga
     3841 agccgtagcc gatacgaaga cccgccaggc cgtaaatctt cgagaacgta cgaagcgtga
     3901 tgacgttatc gtaccggtag tgcatcgaat ccggatagcg cggattgtct ttggcgtact
     3961 caaagtacgc ctcatccaga atgatgagca cgcgctcggg aatatggcgg tagaaacgat
     4021 cgaattcctg acgggtgaaa atcgtgccgg tgggattgtt cgggttcgcc aggtagatga
     4081 tcttggttct atcgttcacc gcggcggcga gggcatccag gtcatagtgc cactcgcggt
     4141 acggcaccgt ccggtacctt accccgcgcg agcgggccag cacctggaac ccgatgaacg
     4201 cggattccgt ggtgagcact tcgtcctcat cacaaaggaa cgtccggata atgttggaca
     4261 tgataccttc cgagccgctc ccggcgatta cgttttccac cttcagatcg tagcggcttg
     4321 ccagttcgcg ccggagtcca aggcctccat cgggatagag attgagacgc ggcaggcaag
     4381 tggcgatggc atctgtcgcg agcggcgacg gacccagggg attttcgttt gacgccatct
     4441 tgaccacatg cgcgagatgg aactgcttcc gaacctcttc ggcgttgcgg ccgggttcat
     4501 acggccttag atttttgata tacggcggaa cgagcggcat cggcgatccc agtgcttact
     4561 ccatcctaca accacctgaa ggtggttgta ggatgagtcc ggtgttgcag aacgggtccg
     4621 cgggactatg atgaggtagt ccggggaaag aagacactcc gtcccggcag aaagaaggct
     4681 ggcatgaaca tggcagcttt ggatgcatcg gaaagaaagc ccagtatggt ggatcgtatc
     4741 atcggaatgt tcaatatctt cctggacccg gcgggcgcgg ccaaaaatat tcgtctttcc
     4801 tggtcgtggg tggcgccatt ggtgttgagc agcatcgtca tcggcatttg cgggtatctg
     4861 ctgattccga cgaccctccg gatcatgcaa atgaatccgc cacccaatat gacggcggag
     4921 cagttcgaga aggcgcgtcc cataattgaa atgagtcaaa aagtgagcgc gctcgcctcg
     4981 ccggtgatca ttggtgtttt cacggcgttg tttgcgggca ttctgacagc ggcgtgcgcg
     5041 gtcctggata ttcgctcgcg attccgggac catttctccc ttttggcgca cacggggttg
     5101 atcggcgcgc tgggttacct gtgcggattt ctggtcatcc ggcttcgcgg agatgcggtc
     5161 caatctttgc tggaactgag gccgggtttc ggactcgacc tgttcctggg ggagcatgcg
     5221 aacaaatttg tttacgggct gctcaacttc ttttcgcttt ttcaattgtg gaatatcgtg
     5281 atgctgggcc tgaccttcgc gttcctgaca aacgtatcga aaggcagggc gttcgcagcg
     5341 actgcgcctg tttggctgct ggggctggta ttggcgctgg taggcgtgat gtttcagcga
     5401 tgaccgtata attcttagtg aagacatgaa cgctgggaaa atcgaagaag tcacggaagc
     5461 ggcattgccg gccgttccgc tgaccgttga aggttcgagt gtattgcacc agatgctgcg
     5521 gtttcgctgg gcgcaatgga aggcgcttgc tcccgggcgg cgccgggaaa ttgccgccga
     5581 agcggccagc gcgctcgaac agatggagca gccggggcgg aaacaaagcg ccgtctattc
     5641 gctgctgggg cacaagggcg acctgatgct g
//
LOCUS       DASTMK010000145         6071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_391042, whole genome shotgun sequence.
ACCESSION   DASTMK010000145 DASTMK010000000
VERSION     DASTMK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391042"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFQ79_06085"
     CDS             <1..214
                     /locus_tag="VFQ79_06085"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU0139257.1"
                     /translation="AGPGLQDATRLALSDYEIWSDILQTNTGNIRAALREYIDRLRQF
                     EESLNSDATGAYFRSAAAFARLLRER"
     gene            complement(221..538)
                     /locus_tag="VFQ79_06090"
     CDS             complement(221..538)
                     /locus_tag="VFQ79_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEU0139258.1"
                     /translation="MSKKYRSDAMAAIHETVEALHKVGAIDKQTMRRFDDACLTPVQP
                     LSPKQIRALREREHVSQTVFANYLNVTPNLVSKWERGEKRPSGSALKLLSLVEKHGIA
                     AVA"
     gene            complement(516..923)
                     /locus_tag="VFQ79_06095"
     CDS             complement(516..923)
                     /locus_tag="VFQ79_06095"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0139259.1"
                     /translation="MERRPGSPRVFKTRWFERFARKEKIRDRVLVDAVARAEGGQVDA
                     DLGGGVIKQRIARPGEGKSGGHRTIIFFRRGARAVFAYGFAKSDRANIDADEEKQFKE
                     AARHVLRLTEKQIEELVKNGGFVELRYEQKISK"
     gene            1203..2435
                     /locus_tag="VFQ79_06100"
     CDS             1203..2435
                     /locus_tag="VFQ79_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU0139260.1"
                     /translation="MKQVVAIALVLWVLPAFGNQDVEINVNSRYTVEDVEVQAKNKSR
                     LSPALLADIQALVGEKLDHKLLDEIGKRIRGALRVHTVVTRVARGSSPEYVRVIFEAR
                     QRPNDIDISAPKFLYHSRQGWSGVLEVKQPVNANEFSFGILSDGDELLERFAGLKAGY
                     RRMSLGTDNVRLGFQFESYHQQWNRATLIALEQNPDVPGIYRTRQNFEPSMTVIPAGS
                     ITWTFGASFQRFQTQFPAARTEAANAVVNSLRYHRSWEGSENQHDLAAGYSLRAATTL
                     LDTDFVYTRHTWDAVYSFTRGRNHVLARFNAGLLSGRAPLYERFVVGNTSTLRGWNKF
                     DLDPLGGTRLAHGTVEYGYRIFRIFYDTGSVWDRREEPDVQHSLGLGFRKGRFMLALA
                     FPVKDGRAEPIFMAGMNY"
     gene            2436..3053
                     /locus_tag="VFQ79_06105"
     CDS             2436..3053
                     /locus_tag="VFQ79_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139261.1"
                     /translation="MNSAAGLHFVTRRKWLLAGFVFFPHAGPAAAELVVRPKSGGLHV
                     SAPGLHFLAGKSLERLRDGASVFFDFQLALLDEAKVRMLARTLERFAVSYDLWEEKFA
                     VTSVQPVRKPVSNLSARAAESWCIEQLMVSTESLSPQRPFWVRLDVRSEDAGERAPVV
                     GDPGINLTSLIEIFSRTTRSQNPRWSVEAGPMRLGDLRQSGGKDR"
     gene            3055..4536
                     /locus_tag="VFQ79_06110"
     CDS             3055..4536
                     /locus_tag="VFQ79_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0139262.1"
                     /translation="MSVRARLIAVFVAATVVPLGLTLWMTTSLLEHSLTYASTRELDE
                     ISRSLETTGREYYQQARQELAAAVERGEVKPVRMLPENRAKWPPAVAEFSDSGEDTRF
                     NLAGEEGDRLDYLVRRGAAVDVYSKDLGAVAMDRLSEQYARARSLIETAKARDLRRGF
                     TYTYVLLMSAIWMVSLLVLVYLASRISRPIGELTNSLLKLSAGDLSVRLHTDRRDEVG
                     KAIRAFNHMAGQLHHSRERLVFLTRLAGWQQLARKMAHEVKNSLTPIRLTIEEISARG
                     ADGDRAFLEQAAQIVVDEVNSLERRVRAFSEFAAEPPVRPAILNVNALLEERVAFLRT
                     AHPEVAYQLRLVEDAPQAFADEDLVKGILTNLLENAAEAAGAGGAVLAATRVERDTGQ
                     EMIALEVHDSGPGLSMQAIETLFEPAISFKKGGMGLGLSIARRSALLCGGDIVLIQGE
                     LGGAAFRLLLPARESPMPDDINDPSCPQHELSSSTTKKISAAR"
     gene            4479..5825
                     /locus_tag="VFQ79_06115"
     CDS             4479..5825
                     /locus_tag="VFQ79_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0139263.1"
                     /translation="MPATRIVVLDDEENIGRSLKLILERENYSVTVCRTATEFQARAE
                     ARRGDAYLIDVRLPDGNGIDILRSMRQLGITAPVVMISGHGTIADAVESTRVGAFDFL
                     EKPLSRDRVLLALKNALEQSNLRAENERLRELVGSAPRMIGSSAPFTRVVEQATMAAR
                     SDARILLIGESGTGKELLAAHVHRESPFSSGPFVKVNCAAIPTELIESELFGHEKGAF
                     TGAVSARRGKFELADGGTIFLDEVGDLHGASQAKLLRVLQEGEFHRVGGEQPIRVSVR
                     VIGATNRDLEEMVAQEKFREDLYYRLSVVPIRVPPLRERVEDIAPLADYFLVEFCARN
                     NFRARKIEPEVYAILSRYPWPGNARELRNAVERMAILTPGETLTSSSVPVEIRYPKTS
                     QPRSTIQEARHSAERDHVLRALEQANWNVSNAARTLGMERTNLHKRIRALGISRQR"
     gene            5871..>6071
                     /locus_tag="VFQ79_06120"
     CDS             5871..>6071
                     /locus_tag="VFQ79_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HEU0139264.1"
                     /translation="MALTRRQKEVLDFICDFVTENGFSPSYEEIAGGLQLASLATVHK
                     HIAALELKGYLRHNFNQSRSVDV"
BASE COUNT         1159 a         1834 c         1849 g         1229 t
ORIGIN      
        1 ggccggaccg ggcctccagg acgcaacccg cctggccctg agcgactacg agatctggag
       61 cgatatcctc cagacgaata ccggcaatat tcgcgcggcc ttgcgcgaat acatcgaccg
      121 gttgcggcag ttcgaggaat cgctgaactc ggacgccacc ggtgcgtact tccggagcgc
      181 ggccgccttc gcacgcctcc tgcgcgagcg gtgagagttt tcaggctact gcggctattc
      241 cgtgcttctc gaccagagac aacagcttga gcgccgaacc ggatggccgc ttctcgcctc
      301 gctcccattt actcaccagg ttcggcgtca cgttgaggta gttggcaaag accgtctgac
      361 tcacgtgctc ccgctcccgc aacgccctaa tctgcttggg cgagagcggt tgcactggcg
      421 tgaggcaggc atcatcgaag cgacgcattg tctgcttgtc gatggcgccc accttgtgta
      481 gcgcctccac agtttcgtgg atggcagcca ttgcgtcact tcgatatttt ttgctcatat
      541 ctcagctcca caaaaccgcc gttcttgacc agttcctcga tctgtttctc cgtcaaccgc
      601 agcacgtggc gtgcagcctc cttgaactgc ttctcttcat ccgcgtcgat gtttgccctg
      661 tcgctcttcg cgaaaccgta cgcgaaaacc gctcgcgcgc cgcggcggaa aaagatgatc
      721 gttcggtgcc cgcccgactt gccctctcct ggccgggcga tcctctgctt gatgacgcca
      781 ccgccgaggt cggcatccac ctgtcctcct tcagcacgcg ctaccgcgtc caccagcacg
      841 cggtcgcgaa tcttttcctt tcgagcaaag cgctcaaacc acctcgtctt gaagactctc
      901 gggctgcccg gtcggcgttc cacacgcctc cattgtatag tacctggtac cacatttcca
      961 tcaaccgtgt tcaaccagcc aacaagcgcg cccacgaaac taccagccgc cccccccggg
     1021 tgtgcatttc cgggaatgca gcatgagtcc gcgcgagccc tgcgaacgtc tccggcgaag
     1081 tacacaccta taacgccgtc gtcccgtcac agcgtgaatt cccttcacta cggccgccgg
     1141 gtttttcctt gtaatggttg gcttttgcga atggcatggc aactgcttca caggtaaatc
     1201 gtatgaagca agttgtcgct atcgcgctgg tgctgtgggt acttccggcg ttcggcaacc
     1261 aggatgtcga gatcaacgtc aactcccggt ataccgtgga ggacgtcgag gtacaggcga
     1321 agaacaaatc gcggctaagc cccgcgttgc tggcggatat tcaggcactg gtcggggaaa
     1381 agctggacca taagctgctc gacgagatcg gcaagcgaat ccggggagcg ttgcgcgttc
     1441 atacggtggt gacgagagtg gcgcgcgggt cgagccccga gtacgtgcgg gtgatcttcg
     1501 aggcgcggca gcggccgaac gatatcgata tctcggcgcc caaattcctc tatcactcca
     1561 ggcaaggctg gagcggcgtg ctggaagtga agcaaccggt gaacgccaat gagttcagct
     1621 tcggcatttt aagcgacggc gatgaactgc tggagcggtt tgccggcctg aaggcgggat
     1681 accggcgaat gtcgctcggg accgacaacg tccgcctcgg cttccagttc gaaagctacc
     1741 atcagcaatg gaaccgggcg acgctcatcg cattggagca gaacccggac gttccaggca
     1801 tctatcgcac acggcagaat ttcgaaccgt cgatgactgt gattcccgcg ggttcgatca
     1861 cgtggacatt cggcgccagc ttccagagat ttcaaactca gtttccggcc gcgaggaccg
     1921 aagccgctaa tgcggtggta aattccctgc gctatcaccg ttcttgggag ggttcggaga
     1981 accagcacga cctggctgca ggttacagcc tgcgcgcggc caccacactc ctcgacaccg
     2041 atttcgttta tacgcggcat acatgggatg cggtctacag ttttacacgc ggccgcaatc
     2101 atgttttagc ccgtttcaac gcgggtctgc tttccggccg tgcgccgctg tatgagaggt
     2161 tcgttgtggg aaatacatcc accctgcgcg gctggaacaa gttcgatctg gacccgctgg
     2221 gcgggacccg gctggcgcac ggtacagtcg agtacggtta tcggattttc cggattttct
     2281 acgacacagg ttccgtctgg gaccggcgtg aagaaccgga tgtacagcat tctttgggac
     2341 tgggcttccg caaagggcgg ttcatgctgg cgctggcatt tcccgtgaaa gacgggcgtg
     2401 ccgagccgat atttatggca ggtatgaact attaaatgaa ctccgcagcg gggctgcact
     2461 tcgtcacccg gcgaaagtgg ctgctggccg gcttcgtctt cttcccccac gcggggccgg
     2521 ccgccgcgga gctggtggtg cgcccaaagt ccggcggcct gcacgtctcc gccccggggc
     2581 ttcatttcct ggcgggcaag tctctcgagc ggctgcgtga cggagcatcg gtgtttttcg
     2641 atttccagct tgctctcctg gatgaggcca aggtgaggat gctggcacgc accctcgaaa
     2701 ggtttgcggt aagctacgat ctctgggagg agaagttcgc ggtaacgagc gtgcagcctg
     2761 tccgaaaacc cgtctcgaat ctgtccgccc gcgccgccga gagctggtgc atcgagcagt
     2821 tgatggtgtc taccgaatcc ctttccccgc agcgtccgtt ttgggtcaga ctggatgtcc
     2881 ggtcggaaga tgcgggggaa cgtgcccccg ttgtgggaga tccgggtatc aatctcacca
     2941 gcctgattga aatcttcagc cggacgacac gatcccagaa tccccgctgg tccgtggaag
     3001 cggggccgat gcgtttaggc gacctgcgcc agtccggtgg taaggaccgg tgatgtgagc
     3061 gttcgtgccc gtctgattgc ggttttcgta gccgctaccg ttgttcctct cgggctgacg
     3121 ttgtggatga cgacgtcact gctcgaacac agtctgactt acgcctccac gcgcgagctg
     3181 gacgagatct cccgctctct tgagaccacc ggacgcgagt attaccagca ggcgcgccag
     3241 gaactggccg cggccgtcga gcgcggagaa gtgaagccgg tgcggatgct gcccgagaac
     3301 cgcgcgaaat ggccccccgc tgtagcggag ttttcagaca gcggggaaga tacgcgcttt
     3361 aatcttgccg gagaggaagg cgaccggctg gattacctgg tgcggcgcgg cgcggcagtg
     3421 gatgtctact cgaaggatct cggcgccgtt gccatggacc gcctgtccga acaatatgcc
     3481 cgcgctcgca gcctcatcga gacggcaaag gcccgcgacc tgcgccgcgg cttcacctat
     3541 acctacgtgc ttctgatgtc cgctatatgg atggtttcgc tgctggtgct cgtctatttg
     3601 gcctcgcgaa tcagccgccc cattggagag ctgacgaaca gcctgttgaa gctgtccgcc
     3661 ggtgatctgt ccgtgcggct tcatacggac cggcgcgacg aggtgggcaa agcgattcgg
     3721 gcattcaatc atatggccgg gcagcttcat cacagccgcg agcgcctcgt atttttgacg
     3781 cgcctggcgg gctggcagca gcttgcgcgc aagatggcgc acgaagtaaa gaactcgctg
     3841 acgccgatcc ggctgacgat tgaggagatc agcgcgcgag gcgccgatgg cgaccgcgca
     3901 tttctcgaac aagccgcgca gatcgtcgtg gacgaagtga acagcctgga acgccgtgtg
     3961 cgtgcgttct ccgagttcgc cgccgaaccg cccgtacgcc ccgctatcct gaatgtgaat
     4021 gcgctgttgg aggagcgggt cgcgttcctg cgaacggcgc atccggaggt ggcctaccag
     4081 ttacggctcg tggaggacgc cccacaggcg tttgcggatg aggatcttgt gaaaggaatc
     4141 ctgacgaacc tgctggagaa tgcggccgaa gccgccggcg ccggaggtgc tgtcctcgcc
     4201 gccacacggg tcgaacggga tacaggccag gagatgatcg cgctggaggt tcacgattcc
     4261 ggtcccggcc tgagtatgca ggcgatcgag accttattcg agccggccat ctctttcaag
     4321 aaaggcggaa tggggctggg cctgtccatc gcacgccgca gcgccttgtt gtgcggcggt
     4381 gacattgtac tgattcaggg agaactcggc ggggcggcct ttcgtcttct gctgccggcc
     4441 cgtgagtctc ccatgcccga cgacatcaac gatccatcat gcccgcaaca cgaattgtcg
     4501 tcctcgacga cgaagaaaat atcggccgct cgctgaagct gatcctggag agggaaaact
     4561 actcggtgac ggtctgccgg accgccaccg agttccaggc ccgcgcagag gcgcgacgcg
     4621 gtgatgccta cctgatcgac gtgcgcctgc cggacggcaa cggtatcgat attcttcgat
     4681 cgatgcgcca gctcggaatc accgcgccgg tcgtcatgat ctcgggacac ggaacgattg
     4741 ccgatgccgt ggaatccact cgcgtgggcg cgttcgactt tctcgaaaag ccgttaagcc
     4801 gcgacagggt cctgctggcg ttaaaaaacg cgctggaaca atccaacctg cgagccgaaa
     4861 acgaacggct gcgcgagctg gtcgggtcgg ccccgcggat gataggaagc tcggccccgt
     4921 tcacacgcgt tgtggaacag gccaccatgg cggcacgatc cgacgcgcgc attcttctga
     4981 tcggagagtc cggcaccgga aaggaactgc tggccgccca cgttcatcgc gaaagcccgt
     5041 tctcttccgg gccgttcgtc aaggtgaact gcgccgccat tccaaccgag ctgatcgaga
     5101 gcgagctttt cggccatgag aaaggcgcgt tcaccggggc tgtgtccgcg cgccggggca
     5161 agttcgagct ggccgacggt ggaacgattt ttctagatga agtcggcgac cttcacgggg
     5221 cttcgcaggc caaactgctt cgtgttttgc aggaagggga atttcatcgg gtaggcggtg
     5281 agcagccgat ccgcgtctcg gtgcgcgtca ttggcgccac caaccgcgat ctcgaggaga
     5341 tggtggcaca ggagaagttt cgagaggatc tgtattaccg gctctcggtg gttccgattc
     5401 gagttccacc gctgcgggaa agggtcgaag atatcgcacc tttggccgac tattttctcg
     5461 ttgagttctg cgcccgcaac aatttccgcg cgcggaagat cgagcccgag gtttacgcga
     5521 tcctcagccg ctatccatgg ccgggaaatg cccgcgaact gcgcaatgcg gtagagcgca
     5581 tggccattct cacacctggc gagacactga cctccagttc ggttccggta gagatacggt
     5641 accccaaaac gtcccagccg cgttccacca ttcaggaggc gcggcattcg gccgagcgtg
     5701 atcacgtgct ccgcgcattg gaacaggcga actggaacgt ttcgaacgcc gcccgcacac
     5761 tgggaatgga gcgtacgaat ttgcacaaaa gaatccgtgc gctcggcatc agccggcagc
     5821 gataggttct ttcctattga cttttccttt ttcttcgcca aaaatgaagc atggcgctaa
     5881 ccaggaggca aaaggaagtc ctggacttca tctgcgactt tgtcacagag aacggcttca
     5941 gtcccagtta cgaagaaatc gcaggcgggc tgcaactggc ttcgctggca accgttcaca
     6001 agcacatcgc tgctctggag ttgaagggat acctgcggca caacttcaac cagagccgtt
     6061 ccgtcgatgt c
//
LOCUS       DASTMK010000146        14331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_519823, whole genome shotgun sequence.
ACCESSION   DASTMK010000146 DASTMK010000000
VERSION     DASTMK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14331
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519823"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFQ79_06125"
     CDS             <1..331
                     /locus_tag="VFQ79_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139265.1"
                     /translation="CAGAGLVSDFVRSPLNSRLRSRSVRLDLDHHADGYHEGEFHSTG
                     TPDLASAMGAASSPNTAAFGTAVVVPGWPLTLFKTISWLPPAVGDAPPAAYFCFTSPG
                     RPPPSLL"
     gene            503..1183
                     /locus_tag="VFQ79_06130"
     CDS             503..1183
                     /locus_tag="VFQ79_06130"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU0139266.1"
                     /translation="MKRILSGLSRFQNEIFPQTRQLFERLASSQTPEALFLTCSDSRI
                     VPELMTQTAPGDLFICRNAGNIAPAYGEVNGGVSATIEYAVLVLEVKDIIICGHSDCG
                     AMKGLLHPEQLAAMPNVAAWLKHAESARFMVQDNYPHLKGADLLNALIEENVVAQLEN
                     LRTHPSVASRLRKGKLQLHGWVYQIDHGTVTAFDPELTRFVPIGKTTDESENGFTRGA
                     AAVEGANV"
     gene            1176..2576
                     /locus_tag="VFQ79_06135"
     CDS             1176..2576
                     /locus_tag="VFQ79_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEU0139267.1"
                     /translation="MSSSAQVANVQVSLGPKPEATTPDAAPLFKRLLYYINPVDGRYE
                     DLRKGNRALNVFRDFTAGLIVAMVAIPLAMGFAMASGLRPEQGIVGGAIAGLIGALWG
                     GSKYQVYGPTAAFIPVIGGLMAKYDHGVLVLASVLAGLILMGFGVARLGRIVAKVPHS
                     IVVGFTIGIAITIALSQIGEVLGLKAKMGYGFFEKLGVIAANFGAINYFAVILGLGTF
                     LLTKYILKVSVFIPAPLLAIGVTTVVAATMWSDKGFTLVRDRYGYIPTDFWVFTKPAA
                     ITWNAQFLGDLTYFVIAIVFVAAVESLLCSRMADRLADNRGMPYNPNKELWGQGLVQV
                     VIPLLNGFPHTGALARTATNIKLGAISPLAGVFKCFLKLGLALYLASYLELVPMACIG
                     GILLYVATAMVKPAEVKQVLAHNRFHIGLMVYTAVMVVVTDFLTGVLSAVILWGLLHR
                     LLDKPVPAAEQALAQS"
     gene            2598..3473
                     /locus_tag="VFQ79_06140"
     CDS             2598..3473
                     /locus_tag="VFQ79_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0139268.1"
                     /translation="MALFHRVMVALARAESDRSLISYARLVAGMAPGAEFHFIHALDW
                     PEESRGLQMVNHGQAVSQIKSLVEEQFGNSGQCYVRVLPGDRTDQLLEYAAEWSVDLI
                     LMGHRRNRNGRRSMARRLAMKAPCSVWVVPEGSAVKISKVLAAVDFSKHSASAMYTAA
                     AITRDNRLPECVAVHVHFGGDGARPPSPQDLSDFITQSNPHNVRVKPVVTAAATVSEA
                     VHRLMPAESLDLVVLGSRGSSDSSSILLGAESEQVIMQSPIPVLVVKQPGERLEVLEV
                     LLDRTLCLREEARPA"
     gene            3509..4414
                     /locus_tag="VFQ79_06145"
     CDS             3509..4414
                     /locus_tag="VFQ79_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0139269.1"
                     /translation="MAYNRHVQPFRKILVALAGIDTDKQLLDYAAMLAGISAGAELHC
                     VHVTGASSAGSRWIASALESEVRAALEPLPSGQVSCHVLEGERTDALLSFATTAGTDL
                     ILLGHRRNRSGRRSLARRLAMEAPCSIWMVPEGSPASLRTILVPIDFSRRAADALSVG
                     TAIAEAAGLDECLALHVYFNKATVTFDEYDEIIAEEKGQAFCLFVAPIDLHGVHAKPL
                     FVEGVNVADTINRVAAEQKADLIIMGTRGRSRSAAVLLGSETEQTIIDTKIPILAVKH
                     FGARRKLVEVLLDKRLRQRGDQRFT"
     gene            4501..6093
                     /locus_tag="VFQ79_06150"
     CDS             4501..6093
                     /locus_tag="VFQ79_06150"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEU0139270.1"
                     /translation="MAIVAGVDFGTQSVRVSIVDSQRGRLGSGAVDYPLHRRKQDPDY
                     ATQSHADQMRALVEAAGKALAAAGVPGGSVEALAIDTTGSTVIPVGEGLEPLDEYYLW
                     CDHRAWREAAGITETAHRLGMEAINWSGGAYSSEWGFSKVLHWLRNNPSKRDKLVTAL
                     ENCDMVTAELCGIREPSRVPRSICAMGHKWMWNEALGGLPPEDFFVAVDPLLAGIRDK
                     LNGRYATSNTIAGELSPEWADRLGLRAGIPIPVAGIDAHWDAIGAGCRVGDVVNVIGT
                     STCIMAIGERISLVPGVCGVVEGSIHPGMTGVEAGLSATGDIFDAIARRAGSTVAALS
                     DGLEKYQAGQTGLLRLTWDNGDRTVLVNPELGGITLGWKLTHTAQDELFAAIEGTAFH
                     TRIILERMAEHGVPIQRVINGGGIPQRNEVLNRVYANVLNKPVLVPESEVTSLGSAIF
                     AFLAAGTFQSVEEAQDALCPKYRVVEPDGAATRVYGELYELFRRVYFGFGVPSANPMK
                     AGDVLSALRRIAGEVQMAAGAA"
     gene            complement(6101..9055)
                     /locus_tag="VFQ79_06155"
     CDS             complement(6101..9055)
                     /locus_tag="VFQ79_06155"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEU0139271.1"
                     /translation="MRWTVLALLITLSFAGAARAAVTVLENFEGTTPSGSWTGGKVEV
                     SEEQSSHGNRSGRITFEQSRGEIRLQPRQRDWRPFDRLLFDVYSDRSEIRTFSLRIYE
                     RTNGAGLDDYFEARNKIVVKKGWNHIEVKLPGMEAASYQRKLSLGNIEELSFFAEGMR
                     FPWTVFVDNIRLVQGQEDEATKSRSEPDDIVTTIDNRWFTVRQVARPEEVPESDDVAR
                     LRKEAHQQLDRLRETIRAAQVQGIDTIYAERHLVTAELGLQVRPRLPWFNNDADKTKM
                     FTYVAESCVRGRRHLEDLIAGVVRLPHVDDTQATGPDVPLYPNLKGLKSDGWFYRDSE
                     GKPLMVISLHSPSTLLQRFFATPRQHIESYTVGGGSRWTIEESPVYEAFHKYPDTHRV
                     GWDGWCGHLIRDLFSMGGSKMENVVICLESPHIRDAVDKYIRINIPKFHNNPQLLYDV
                     MAYELMYICYCERSQKMFREWLANKHGTIARVNEIYGTSYTGFDGIVAPPVKNSRPLA
                     GTNRALWYDWTRFNQDRFTDYLLWVRSRVRAVDSTVPLAAGGSSSMLGGGTGTSGIDE
                     ERIVNEVGDLILHEGGGSTLGVDLQMALPETKKPLADPEMSLQSVYNLLPHALHGKSV
                     MQFFHWPVQPQNEFFSMNASALPHSWRFSLAEVGDLMRSTLDARRLAPEIAAFVDAPA
                     EVAILYSQTSTLQLPPEMLTWRATPYLAELGKMYEGSRYLDAKTTFITERQAAKGWLS
                     RYKLLLVPGVRNLPAGVVQKIWEFAESGGRVLITPESLLGDEYNRQQPFLSKIGIAIE
                     QTRHPKPGTIGAMTQGYDQSFSQDVAFTGGSSQKLRASAWDGMELETGGVSQKLQLTG
                     DAESIASFSDGGTALARTRTGRGVVYYSAASLTDKSYWRLLDRIFHDAGVTRPVRVQD
                     ADDPEAWKLESRYVVSGNRRLLYVTSHKNAPVRVEITNAPEGAFVFDLRDRIAVENIT
                     LPARETGIYELR"
     gene            complement(9052..11052)
                     /locus_tag="VFQ79_06160"
     CDS             complement(9052..11052)
                     /locus_tag="VFQ79_06160"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0139272.1"
                     /translation="MMLLRCLLPFLCLSSVFAQSRNTVEERYKAFENYLVRRAMAVTG
                     SFPGGIKTLDQWKQVRPDVRKRFLYTMGLDPMPKRTPLNARVMGQFERDTYRVEKIVF
                     ESMPGLYVTGNLYLPKKPAGKLPTVLYLCGHSPGPWGAKVRYQHHGIWLARNGYAAFL
                     IDTIEFGEIPGIHHGTHDLEMWDWLSLGYTPAGPEVWNAIRALDYLETRPEVDTSKLG
                     VTGISGGGAMTWYTAAADDRVQVAATVCATWTTGHHTALDAVHENCDCIYFVNTFLDD
                     LPAVGALIAPRPLKIISARRDPSFPTAGYHDAFERIRSIYQLYGAEEKVVEYDHDAPH
                     ADILPFRKEANEWLNRWLKNDNTPFEEGKIEEEKPEVLTVLERHPENAINGHIHKTFI
                     ASPAVKPPATLNAWKTRRATILSELKDKSLRAFPAATVPFNAAKVASKDWATNYTDAF
                     RVEFATEEGIRVTGQLYVPRGAKPEYPGLIYVKGRQDIVYPVDYDLLLPAWGNHVVLV
                     VQPRATDYPMNNYKIATVKRTAALIGATVESMQLWDILRSIDFLLKESGAKVTSVSLY
                     ARKDMTVPAMYAAALDERVSRLIVEDPPYSHWQGPAILNALRFTDLPEVAAMVAPREI
                     VALTPLPSAYGLTRTVYALYGKKGGMRQAPGLAGALQVWKHQ"
     gene            complement(11105..>14331)
                     /locus_tag="VFQ79_06165"
     CDS             complement(11105..>14331)
                     /locus_tag="VFQ79_06165"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0139273.1"
                     /translation="GYGQATQGSILGNVMDATGATVPGAMVTVRNEGTNFTREVKTDD
                     SGDYRVSGLEPGNYEVTITSQGFKTSKQTKVDVVANQIKRVNATLEVGDVSTTVTVEG
                     GTAQVETETATLSNVKTSRDFTELPLSIYGRGWANITNVTAGIQSQQCCAFLANGARG
                     SGVNFTTDGIYFMNTISSSQGPNGFSGEIEFIQEVKVMTANNSAEFAQVSQFAAVSKA
                     GTNELHGSLYWGNFNNKFSARQWQDTQKPEFTNHNMFAITNGGPVYIPRLYDGRNKTF
                     YFFSYGGARYRVGSRTRVSLPTPAFRQGDFSSIASLVTIVDPLNGQPFANNRIPANRI
                     SPVSAALQDIIYPDPNQPGQGDYSLTENFYADPGGRFDSDVYSIRGDQKISDKNFLYA
                     RVGLTINNKDSYPGPLKDGYGPGAWQGNHPARSVVISDTHTFSPAVVNEIKLGYSRDF
                     GYWFDTNYGRDVLSQIGLQGISNPGNDPAIGGMPSLSFGGSVGFAGTDTWANGNFQAQ
                     NTYQLIDNISWYRGRHNLKMGFDVRWYQINDQSKPQSMRGAFTFDDQLSGFNYANFLL
                     GYPSFATRSIARPNAYPRSSQYMFYVQDDFKVSPRVTLNYGLRYEYQTPWVEKFDRMF
                     TFDPTRASLVTAGDSIPTDLVPAVAATLPIISAAEAGYPVRSLMKTDSNNITPRLGLA
                     VRPFSDASTVVRLGWGIYSQMWPGSIGLGNTGGPWQSEEAFFIEGNTPTIRFPNPFLT
                     TSEFAGLQSVSGLSPSFPTERTQQWSASFGRQIWQTAVDIAYVGTSVKNIPYTEDLNL
                     LAPSATPYNSARRPYQLFNSVNFVQTGGSSIYHGMTITANRRMGDGLWYNVNYTWAKA
                     LTDTDLGNFAQGAQQNQYQRFLERADEQRHRRHQLRFSYVYELPVGRGKHFGSNMPTV
                     ANWVIGNWNVSGITTFVTGQVLSPAFSGTDPANTNQFGGRPDRIGDGNFDSGEMRDRI
                     KSREPIFDSSAFVRPETGRGFYGNSARYILTGPGEMTWNTVLSKNFPIAAERARLQFR
                     WEMFNALNRPNFGNPNTNLQSGAFGLVTGAGSARSMLFGLRLDY"
BASE COUNT         2736 a         4179 c         4382 g         3034 t
ORIGIN      
        1 gtgtgcgggg gcagggctcg tctcggattt tgtacgctct cccctcaact ctcgccttcg
       61 cagccgctcg gtccggctcg acctcgacca tcatgccgat gggtaccatg agggcgagtt
      121 ccactccacc gggacgcctg acctcgcttc ggcgatgggc gcggcaagtt cacccaacac
      181 tgccgctttc ggaaccgcgg ttgttgtgcc gggatggcct ttaacactct tcaagaccat
      241 aagctggctg ccgccggcag ttggggatgc gccgccggct gcctatttct gcttcaccag
      301 ccctggacgt cctccgccgt ctctccttta acaactcgct ttatacaggt tccgtttgca
      361 cccgtagtgt acgtccgcgc gtgtggctct cacgcgcggt gaacggggct tgggggagag
      421 ccatatgaaa aattcatatg ccctgcggtc cacggccggg aacgccgtcc gtgtgaagca
      481 ggaggacgaa ggagttctat aaatgaagcg aattctaagc gggttatccc gtttccagaa
      541 cgagatcttt ccacaaaccc ggcaactgtt tgagcggctc gcatccagtc aaacgcccga
      601 ggcgttgttc cttacctgct cggattcacg gatcgtgccc gagttgatga cacagacggc
      661 gccgggggac ctcttcatct gccgcaatgc gggaaatatc gctcccgcct atggcgaagt
      721 gaacggcggc gtgtctgcca cgattgaata cgccgtcctg gtgctggaag tcaaagacat
      781 tatcatctgc ggccattccg attgcggggc catgaaagga ttgctccatc ctgagcaact
      841 ggccgccatg ccgaatgtgg ctgcatggtt gaagcacgcc gagagcgcgc gtttcatggt
      901 gcaggacaac tatccgcacc tgaagggcgc ggatctgtta aatgcgctca tcgaagagaa
      961 cgtggtggcg cagctcgaaa acctccgcac tcacccttcc gtcgcctcac ggctgcgtaa
     1021 ggggaaactc caactgcatg ggtgggttta ccagattgac catggaacgg tgactgcttt
     1081 tgatcccgag ttgacacgct ttgttccgat cggaaaaaca accgacgaat cagagaacgg
     1141 cttcacacgc ggagccgccg ccgtggaggg cgccaatgtc tagtagcgca caggttgcaa
     1201 acgtacaggt ttcattagga ccaaaaccgg aagctaccac gccggatgcg gcaccgcttt
     1261 tcaagcggct gctgtactac ataaacccgg tcgacggccg ttacgaagat ctccggaaag
     1321 gcaatcgggc cttgaatgtt ttcagagact tcacggccgg cttgatcgtc gctatggtgg
     1381 cgattcctct ggcgatgggt ttcgctatgg cgtccggcct gcggcccgag caaggaattg
     1441 tgggaggggc catcgccggt ctcatcggcg ccctttgggg cggctcgaag taccaggtgt
     1501 acggtcctac ggcggccttt attcccgtga tcggcgggct gatggcgaaa tacgatcacg
     1561 gcgtgctcgt cctcgcctcc gttctcgccg gcctcatcct gatggggttt ggagtggcac
     1621 ggctgggccg catcgtggcg aaagtgcccc actcgatcgt cgttggtttt accatcggca
     1681 ttgccatcac gatcgcgctg tcgcagattg gcgaagtcct cgggctcaag gcgaaaatgg
     1741 gctacgggtt cttcgagaag ctcggggtaa tcgcggcaaa ttttggtgcg atcaactact
     1801 tcgcggtgat tctggggctg ggtacctttc tgctgaccaa gtatatcctc aaagtttcgg
     1861 tattcattcc ggcgccgctg ctcgcgatcg gcgtgacgac tgtcgtcgcc gccacgatgt
     1921 ggtcggataa aggtttcacg ctggttcgcg atcgttatgg ctacatcccg acggattttt
     1981 gggtgtttac caagccggcg gcgatcacgt ggaacgctca atttctcggc gacctgacgt
     2041 acttcgtcat cgcgattgtc tttgtcgcgg ctgtcgagag cctgctgtgt tcccgaatgg
     2101 cggaccgcct ggccgataac cggggcatgc cttacaaccc gaacaaagag ctgtgggggc
     2161 aaggcctggt gcaggtcgtc attccgcttc tcaacggctt tccgcacacc ggcgcgctgg
     2221 cgcggacggc aaccaacatt aaactgggtg ccatctcgcc gctggcggga gtcttcaaat
     2281 gctttttgaa gctggggctt gcgttgtatc tggccagcta tctcgaactg gtgccgatgg
     2341 cctgcatcgg tggcattctg ctctatgtcg ccacggcgat ggtgaagccc gccgaagtca
     2401 aacaggtact cgcgcacaac cgattccata taggtctgat ggtttacacc gctgtgatgg
     2461 tcgtggtgac ggattttctc accggcgtac tctccgcggt gatcctgtgg gggctgctgc
     2521 atcgcttgct ggataagccg gtgcccgcgg ccgagcaagc cttggcacaa agctgattgc
     2581 cggaaggagg ttcaagcatg gcgctatttc atcgtgtaat ggtagcgctg gcccgggccg
     2641 aatcggatcg ctctttgatc tcttacgcac ggctcgttgc tggcatggca cccggcgcag
     2701 agttccattt catacatgcg ctggattggc ccgaggagag ccggggactc cagatggtca
     2761 atcacggaca ggctgtatct cagatcaaat cgttagtgga ggaacagttt ggaaattcgg
     2821 gtcaatgtta cgtacgggtg ctccccggcg accgcacgga tcaactgctg gagtatgcgg
     2881 ccgagtggag cgtcgacctg atcctgatgg gccaccgccg gaaccggaac ggccgccgct
     2941 ccatggcgcg ccggctcgcc atgaaagcac cgtgctccgt ctgggtggtt cccgagggct
     3001 cggcggttaa gatctcgaag gtcctggccg cggtcgattt ctccaaacac tccgcatcgg
     3061 cgatgtacac ggcggccgcc atcacgcgcg acaaccggct tccggaatgc gtcgcagttc
     3121 acgtccattt cggcggcgac ggtgcgcgcc ctccgtcgcc gcaggatctc tcggatttca
     3181 ttacccagtc gaacccgcac aacgtcaggg taaagccggt ggtgaccgct gccgcgaccg
     3241 tgtccgaggc cgtgcatcgc ctcatgcctg ccgaaagtct ggatctggtt gtgctcggct
     3301 ctcgcggctc gagcgattcc agttccatcc ttcttggcgc cgaatccgag caggtcatca
     3361 tgcagtcgcc gatacctgtg cttgtcgtca aacagccggg cgaacgcctt gaagtgctgg
     3421 aggtacttct ggaccggacg ctgtgcttac gagaggaggc acgaccggct tgagcgtgca
     3481 gagtagcggt tcaccgcagg cctcgccgat ggcctacaat cggcacgtgc agccgtttcg
     3541 aaaaatcctg gtggcgctgg ccggtatcga cacggataaa caattgctgg actatgctgc
     3601 gatgctggcc ggcatcagcg ccggtgcgga gcttcattgt gtccacgtga cgggagcctc
     3661 ctccgccggc agccgctgga tcgccagcgc cctggaatcg gaggttcgag ccgcgctcga
     3721 accgctgcct tccgggcagg tatcgtgtca tgtgctggaa ggcgagcgga cggacgcgct
     3781 gctttctttt gccactaccg ccgggacaga tctgatcctg ctgggtcacc ggcgcaaccg
     3841 gagcgggcgc cggtcgctgg cgcgccgcct tgcgatggaa gctccgtgct ccatctggat
     3901 ggttccggaa ggctcgcccg cttcgctccg aaccattctg gtgcctattg atttttcccg
     3961 ccgggccgcg gacgcgcttt ctgtcggcac ggctatcgcc gaggccgccg ggctggatga
     4021 atgcctggca ctgcacgtct acttcaacaa agccaccgtt actttcgacg aatacgacga
     4081 gattatcgcg gaagagaagg gccaggcctt ttgcctgttc gtcgccccta tcgacctgca
     4141 tggggtgcat gccaagccgt tattcgtgga aggcgtcaac gtggcggaca ccatcaatcg
     4201 ggtggctgcg gagcagaagg cagacctgat cataatgggc acgcgcggcc gcagccgttc
     4261 cgccgcggtt cttctcggaa gcgaaaccga acagaccatc atcgacacga aaatccccat
     4321 tctcgccgtg aaacacttcg gagcgcggcg aaagctggtg gaggttctgc tggataaacg
     4381 tctgcgccag cgcggcgatc agcggtttac gtaactggtc cggttcgaag atcgtccggc
     4441 aacagccgta ttcgatacac tggtctcgca atcattcgga tttgcgaacc caggagactt
     4501 atggctattg tcgcaggagt agatttcgga acgcagagtg ttcgtgtttc catcgtggat
     4561 agccagcggg gccggctcgg ttcgggcgcc gtggactatc ctctgcatcg cagaaaacag
     4621 gatcccgatt acgctactca aagccatgcg gaccagatgc gggcgcttgt ggaagcggct
     4681 ggcaaagcgc tcgcggccgc tggtgtgccg ggcggcagcg tcgaggcact ggcgatcgac
     4741 accacgggct cgacggtgat tccggtaggc gaaggactcg agccgctgga cgaatactat
     4801 ctctggtgcg atcatcgcgc gtggcgcgaa gcagccggga tcacggaaac cgcccaccgg
     4861 ttgggtatgg aagccatcaa ctggtcgggc ggcgcgtact cctccgaatg ggggttctcg
     4921 aaagttctgc actggctccg caacaacccc tcaaagcgcg acaagctcgt gactgccctc
     4981 gaaaactgtg acatggtgac cgccgagctg tgcggtatac gggagccctc acgcgtgccg
     5041 cgctccatct gtgcgatggg tcacaagtgg atgtggaacg aagcgctagg cgggttgccg
     5101 cccgaggatt ttttcgtggc ggtggatccg ttgctcgccg gtatccgcga caaattgaat
     5161 gggcgctacg ccacgtcgaa cacgattgcc ggtgagttgt cgccggagtg ggcggaccgt
     5221 ctcggcctgc gcgccggcat cccgattccc gtcgccggca tcgatgccca ctgggatgct
     5281 atcggtgcag ggtgccgcgt tggcgacgtt gtcaatgtca tcggcacgtc cacctgcatt
     5341 atggccatcg gcgaacgcat cagcctggtg ccgggtgtct gtggagtcgt ggagggatct
     5401 atccaccccg gtatgactgg tgtcgaagcg gggctctcag ccaccggcga catattcgat
     5461 gcgattgccc ggcgcgcggg ctccacggtc gcggcactat cggatgggtt ggagaaatac
     5521 caggcggggc agaccgggct tctacggctt acgtgggaca acggcgaccg tacagttctg
     5581 gtgaacccgg agttgggcgg catcacgctc ggctggaagt tgacgcatac ggcgcaggac
     5641 gagttgttcg ccgcgatcga gggtacggcc tttcatacac ggatcattct ggagcgcatg
     5701 gcggagcacg gagttcccat tcagcgtgtc atcaacggcg gcggcatccc gcaacggaat
     5761 gaagtgctga accgcgtcta cgccaatgtg ctgaataagc ccgtcctggt accggaatcg
     5821 gaagtgacga gtctcggttc ggcaattttc gcgtttctcg cggcgggaac cttccaatcg
     5881 gttgaggaag cgcaggacgc gctctgcccg aagtacagag tggtggagcc ggatggcgcc
     5941 gccacccgag tgtacggtga gttgtacgaa ttgttccgcc gagtgtactt cggttttggc
     6001 gttccgtccg cgaaccccat gaaagcgggc gatgtgctgt ccgctttgcg gcgtatcgct
     6061 ggcgaagtgc aaatggcggc aggtgcggct tagccgcgtg ttaccgcaat tcgtaaatcc
     6121 ccgtctctct agccggcagc gtgatgttct ccacggcgat gcggtcccgc agatcgaaaa
     6181 caaaggcgcc ttccggtgcg ttcgttattt caacgcgcac cggcgcgttt ttgtggctcg
     6241 tgacatacaa caatcgccgg ttgcccgata caacgtaacg cgattcgagt ttccatgctt
     6301 cggggtcgtc cgcatcctgg acgcgcaccg gtctggtgac acccgcgtcg tggaagatgc
     6361 ggtccagcaa gcgccagtag ctcttgtcag tcagtgatgc cgcagagtag tagaccacgc
     6421 cgcggccggt cctggtacgg gcgagtgcgg ttccgccatc gctgaagctc gcaatggatt
     6481 cggcatcgcc ggtgagctgg agtttctgcg aaacgccgcc ggtctcgagc tccatgccat
     6541 cccaggcgga agcgcgcagc ttctggctgg agccgccggt gaaagcgaca tcctgcgaga
     6601 agctctgatc gtatccctgc gtcatcgcgc ctatggtgcc aggtttcgga tggcgggttt
     6661 gttcaatggc aattccaatt ttggagagga acggttgttg gcggttgtac tcgtcgccca
     6721 gcagcgattc cggagtaatc aggacgcgcc ctcccgattc ggcgaattcc catatcttct
     6781 gcaccacgcc ggccggcaga ttgcgcacgc ccggcaccag gagaagttta tagcggctca
     6841 accaaccctt cgcggcctgc ctctcggtga tgaacgtcgt cttcgcatcc agataacggc
     6901 tcccttcgta catctttccc agttccgcca ggtaaggtgt agcgcgccag gtcagcatct
     6961 ccggaggtaa ctgcaaggtg gaagtctgcg agtataaaat cgccacttcc gccggagcgt
     7021 cgacgaacgc cgcgatctcg ggcgccagcc tgcgggcatc cagcgtggac cgcatcaaat
     7081 cgccgacctc ggcaagcgag aaacgccagc tatgcggcag cgccgacgcg ttcatgctga
     7141 agaactcgtt ctgcggctgc acgggccaat ggaaaaactg catcacgctc ttgccgtgca
     7201 gcgcgtgcgg cagcaagttg tagacggact gaaggctcat ctcaggatcg gcgaggggct
     7261 tcttcgtctc cggcaaagcc atctgcaaat cgacgccgag agtggatcca ccaccctcgt
     7321 gaagaatcag gtcgcctact tcattgacga tacgttcttc gtcgattccg ctggtcccgg
     7381 ttccacctcc caacatgctg gaactgccgc ccgcggccag cggcaccgtt gaatccaccg
     7441 cccggacacg gctacgcacc cataacaggt agtctgtgaa gcgatcctgg ttaaatcgcg
     7501 tccagtcgta ccagagcgct cgattcgtgc cggcgagcgg ccgtgagttc ttgaccggcg
     7561 gcgccacaat cccgtcgaat ccggtgtagg acgtgccgta gatctcgttc acgcgcgcaa
     7621 ttgttccatg cttgttggcg agccattcac ggaacatctt ctggctgcgc tcgcagtagc
     7681 agatgtacat cagctcgtaa gccatcacgt cgtacaacaa ctgcgggttg ttatgaaact
     7741 tcgggatgtt tatgcggatg tatttgtcga cggcgtcccg gatatgcgga ctctccaggc
     7801 agatcacaac gttctccatt ttggagccgc ccattgaaaa cagatcgcgg atcaggtgtc
     7861 cgcaccagcc gtcccaaccc acacggtgag tatccgggta tttgtggaac gcttcataaa
     7921 cgggtgactc ctcgatggtc caacgtgaac cgcctcccac tgtgtagctc tcaatatgct
     7981 gccgtggtgt tgcgaagaag cgctgtagca gagtgctggg ggaatgcagc gaaatcacca
     8041 tcaagggttt gccttccgaa tccctgtaaa accagccgtc gcttttgagt cctttcagat
     8101 tgggatagag cggcacgtcc ggtccggtcg cctgcgtgtc gtccacgtgc ggaaggcgga
     8161 cgacaccggc gatcagatct tccaggtgac gccgcccccg gacacagctt tcggcgacgt
     8221 aggtaaacat cttcgtcttg tccgcatcgt tattgaacca aggcagacgc gggcgcacct
     8281 gcaatcccag ctcggccgtc accaggtggc gctccgcgta aatcgtgtcg atcccctgaa
     8341 cctgtgcggc gcgaatggtt tcgcgaagcc ggtccagttg ctggtgcgcc tccttgcgca
     8401 aacgggctac atcatccgat tccgggacct cctcgggcct ggccacctgg cgcacggtga
     8461 accagcggtt gtcgatggtg gtaacgatgt cgtccggctc gctccgcgat ttcgtagcct
     8521 cgtcttcctg gccctgaacc aggcggatgt tatccacgaa aacggtccag gggaatcgca
     8581 ttccctcggc gaagaacgat aattcctcga tgttgccgag tgaaagcttc cgctgataag
     8641 aggccgcctc catcccgggc agcttcactt cgatgtggtt ccatcccttc ttgaccacaa
     8701 ttttgtttct ggcttcgaag tagtcgtcca gcccggcgcc attggttcgt tcgtaaatac
     8761 gaagagaaaa ggtccgtatt tcactgcgat cgctatagac gtcaaacagg aggcggtcga
     8821 acggacgcca gtcccgttgc cgcggttgaa gacggatctc accgcgcgat tgctcgaagg
     8881 taatccggcc ggaccggttc ccatgcgacg attgctcttc ggagacctcg accttccctc
     8941 cggtccaact gccggagggc gtggtacctt cgaagttctc caaaaccgtt acagccgcgc
     9001 gggccgcgcc ggcgaacgag agtgtgataa gaagtgcaag aacggtccat ctcattgatg
     9061 tttccaaacc tgtagagcgc cggccaaacc aggagcctgc ctcataccgc ccttctttcc
     9121 ataaagtgca tataccgtcc tggtcaaccc gtacgcggaa ggcagcggcg tcaaggcgac
     9181 gatctctctc ggcgcgacca tcgcggccac ttccggcaga tccgtaaagc gaagtgcatt
     9241 gaggatcgcc ggcccctgcc agtgggaata gggcggatcc tccacaatca ggcgcgagac
     9301 acgttcgtcc aaggcggcag catacatcgc ggggacggtc atgtccttgc gcgcatagag
     9361 cgagacggaa gtgaccttcg ccccgctctc cttcaacaga aagtcgatgg aacggagtat
     9421 gtcccaaagc tgcatggatt cgacggtcgc cccgatgagt gcggcggtcc gcttcaccgt
     9481 cgcgatcttg taattattca tcgggtagtc cgtcgctctc ggttggacga ccaggacgac
     9541 gtgattgccc caggcgggaa gaagcaaatc gtaatcgacg ggatagacga tatcctgccg
     9601 ccctttgacg tagatcagcc cgggatactc aggcttggcg ccgcgtggga catacaactg
     9661 tccggtgacg cggatgcctt cttcggtcgc aaactcgaca cgaaacgcat ccgtgtaatt
     9721 tgtggcccaa tccttcgaag ccactttcgc cgcgttgaag gggactgtcg cggcggggaa
     9781 tgccctcagg ctcttatctt tcagctccga gaggatggtg gcccgccgtg tcttccacgc
     9841 gttgagggtc gcaggcggct tgaccgcggg gctcgcgatg aaggtcttat ggatgtgtcc
     9901 gttaatcgca ttctccggat gacgttcaag aacggtcagc acctccggtt tttcctcttc
     9961 gatctttcct tcctcgaacg gcgtgttgtc gtttttcagc catcggttca accactcgtt
    10021 cgcttccttg cggaaaggga ggatgtcggc atgcggcgcg tcatgatcgt actcgacgac
    10081 tttctcttcc gctccgtaga gctgataaat ggaacgaatt cgttcaaacg catcgtggta
    10141 tccggcggta gggaacgacg ggtcgcgcct cgcgctgatg atcttcagag gacgcggagc
    10201 gatcagcgcg cccaccgccg gaaggtcgtc gagaaaggtg ttgacgaaat agatgcaatc
    10261 gcagttttcg tgcacggcat cgagggcggt gtggtgtccg gtggtccagg tagcgcaaac
    10321 ggttgccgcc acctgcacgc gatcgtccgc cgccgcggtg taccacgtca tggctccgcc
    10381 gccggatatg cccgtcacac ccagtttgga agtatccact tccgggcgtg tctccaggta
    10441 atcgagggcg cgaatcgcgt tccagacttc cggaccggcc ggtgtgtagc cgagcgagag
    10501 ccagtcccac atctcgaggt cgtgtgtacc gtggtgaatc ccggggatct ctccgaattc
    10561 gatcgtgtcg atcagaaatg ccgcgtagcc gttgcgcgcc agccagattc cgtggtgctg
    10621 gtaacggacc ttggcgcccc acgggcccgg ggaatgtccg cagaggtaaa gcacggttgg
    10681 caatttgccg gcgggctttt tcggcagata cagatttccg gtgacgtaca acccgggcat
    10741 actttcaaag acgatctttt cgacgcggta ggtgtcgcgt tcgaactgcc ccatcacacg
    10801 cgcattcagc ggggtacgtt tcggcatcgg gtcgagaccc atcgtataga gaaaccgctt
    10861 gcgcacgtcg ggtctcacct gcttccactg gtcaagcgtt ttgatgccgc cggggaagct
    10921 gccggtgacc gccatcgcgc ggcggacgag atagttctcg aaagctttgt accgttcttc
    10981 gaccgtgttg cggctttgcg cgaaaaccga agaaaggcag aggaaaggca ggagacaacg
    11041 taggagcatc atgattacgg gcggggccgg accggtccgg ccccctcttt aaagcacagt
    11101 ctactcaata atccaaacgc aaaccaaaaa gcatgcttct ggccgagccg gctccggtga
    11161 ccaggccgaa cgcgccgctt tgcaggtttg tattcggatt accgaaattc ggacggttga
    11221 gagcattaaa catttcccac cggaattgta gacgggcacg ctccgccgcg atgggaaaat
    11281 tcttcgagag taccgtgttc caggtcattt cgccgggacc ggtcaggata tagcgcgcag
    11341 agttgccgta gaacccgcgg ccggtttccg ggcgtacgaa tgccgaggag tcgaaaatcg
    11401 gctcgcggct tttgatccgg tcccgcatct cgccggaatc gaagttgccg tcgccgatgc
    11461 ggtccggacg gccgccgaac tggttggtat tggcgggatc ggtgccggag aatgcgggac
    11521 tcagcacctg gccggtgaca aacgtggtga tgcccgaaac attccagttt ccgatcaccc
    11581 agttcgccac agtcggcatg ttgcttccga agtgcttacc acggcccacc ggaagctcat
    11641 acacgtagct gaaacgcaac tgatgccggc ggtgccgctg ctcatcggcg cgctccagga
    11701 aacgctggta ctggttttgt tgtgcgcctt gggcgaagtt gcccaaatcc gtgtccgtca
    11761 gcgccttggc ccaagtgtag ttgacgttgt accaaagccc gtcgcccatg cggcgattgg
    11821 cggtgatggt catcccgtga tagatggagg aaccgcccgt ttgaacgaaa ttcacggagt
    11881 tgaagagctg atacggacgc cgcgcggaat tgtacggagt agcactgggc gcaagaagat
    11941 tgaggtcttc cgtgtagggg atgtttttaa ccgacgtccc tacataggcg atatcgaccg
    12001 ccgtctgcca gatctgcctg ccgaaagaag cgctccactg ctgcgtgcgc tcggtcggaa
    12061 agctggggct caggccgctg acgctctgaa gaccggcgaa ttccgaagtg gtaaggaacg
    12121 ggttggggaa ccggatggtt ggcgtgttcc cttcgataaa gaacgcctct tcggattgcc
    12181 acgggccgcc ggtatttccg aggccgatgg agcccggcca catctgcgaa tatatgcccc
    12241 accccaggcg cactaccgtc gacgcgtcgg agaagggccg cacggcgagc cctaggcgcg
    12301 gagttatgtt gttggaatct gtcttcatga ggctccgcac ggggtagccg gcctccgcag
    12361 ccgagatgat aggaagcgtt gcagcaacgg cgggaaccag gtccgtgggg atggagtctc
    12421 ccgctgtcac caggctcgcc cttgtgggat cgaacgtgaa catgcgatcg aacttctcca
    12481 cccacggcgt ctgatactca taccggaggc cgtagttcaa cgtcacacgc gggctgactt
    12541 tgaagtcgtc ctgcacgtag aacatgtact gtgagctgcg gggataggca ttggggcgcg
    12601 cgatgctgcg cgttgcaaag ctcggatagc ccagcagaaa gttggcgtaa ttgaacccgc
    12661 tcaactggtc gtcgaacgtg aatgcgccgc gcatggattg aggcttcgac tgatcgttga
    12721 tctgatacca gcgaacgtcg aaccccatct tcaagttgtg gcgtccgcga taccaggaga
    12781 tgttgtcgat gagttggtac gtgttctgcg cctgaaagtt gccgttggcc cacgtatcgg
    12841 tgccggcgaa gccgacggat ccgccaaaag agaggctcgg catcccgcct atcgccggat
    12901 cgttgccagg attactgatc ccctgaagac caatctgcga gaggacgtcg cgcccgtagt
    12961 tggtgtcgaa ccagtagccg aagtcgcggc tgtatccgag cttgatctca ttcaccaccg
    13021 ccgggctgaa agtgtgggtg tcagagatca cgacactgcg ggcagggtga ttgccctgcc
    13081 aggcgccggg accgtatccg tccttcaaag ggccgggata actgtccttg ttgttgatgg
    13141 tgaggccgac gcgggcgtag agaaagttct tatcgctgat cttctgatct ccccggatgg
    13201 aataaacatc cgaatcgaac cggcctcccg gatccgcata gaagttttcg gtgaggctgt
    13261 agtcgccctg acccggttga ttggggtccg gatagatgat gtcctggagg gcggcggaaa
    13321 cgggactgat gcggtttgcc ggaatgcggt tgttggcaaa cggctgtccg ttcaacggat
    13381 ccacgatcgt cacgaggctg gcgatcgagg aaaagtctcc ctggcgaaac gccggcgtgg
    13441 gaagggacac gcgggtgcga gagcccactc gatagcgcgc accaccgtag gagaagaaat
    13501 aaaacgtctt gttgcggccg tcgtaaagac ggggaatgta caccgggcca ccattcgtga
    13561 tggcgaacat attgtgattg gtgaactccg gtttctgtgt atcctgccat tgccgcgcag
    13621 agaacttgtt gttgaagttg ccccagtaga ggctgccgtg cagttcgttg gtgcccgctt
    13681 tgctgaccgc ggcgaactgc gacacctgcg caaactccgc ggagttattg gcggtcatga
    13741 ctttcacttc ctgaatgaac tcgatctcgc ccgagaagcc attggggcct tgtgagctgc
    13801 tgatcgtatt catgaagtag ataccgtctg ttgtgaagtt gactcccgaa ccccgggccc
    13861 cattcgccag aaatgcgcaa cactgctgcg actgaatgcc cgcggtcacg tttgtgatgt
    13921 tcgcccatcc acggccgtaa atgctgagag ggagttcggt gaagtcgcgc gacgtcttga
    13981 cgttcgaaag cgtagcggtt tcggtttcaa cctgggccgt gccgccttcg acggtaaccg
    14041 tcgtgcttac atctcccacc tccagcgtgg cgttaacccg tttgatctga ttggccacca
    14101 cgtcgacttt ggtttgcttg gatgtcttaa agccttgact tgtaatggtc acttcgtaat
    14161 tgcccggctc gagcccggac acgcggtagt cgccgctatc gtcggtctta acctcccgtg
    14221 tgaaatttgt accctcgtta cggacggtca ccatcgcccc agggaccgtg gccccggttg
    14281 cgtccataac gttgccgaga atgctgcctt gagttgcctg tccgtatcca a
//
LOCUS       DASTMK010000147        13168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_359520, whole genome shotgun sequence.
ACCESSION   DASTMK010000147 DASTMK010000000
VERSION     DASTMK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13168
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359520"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1214
                     /gene="selD"
                     /locus_tag="VFQ79_06170"
     CDS             192..1214
                     /gene="selD"
                     /locus_tag="VFQ79_06170"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU0139274.1"
                     /translation="MASGKLTAHVKAGGCASKLSPKILDRVLAQLPRVADENVLVGFD
                     TADDAGVYKLTPECALVQTVDFFTPVVDDPFTFGAVAAANALSDVYAMGGRPVSALSI
                     VAYPAKGDIEELEQILRGGAEKMREAGCVVLGGHSVADDEIKFGYAVTGIVHPERVKT
                     NAGARAGDVLLFTKSIGIGVVSTALKRGIADPADVEASIASMLTLNRAACEAMLEEDV
                     HGCTDVTGFGLMGHAREMAMASGLTLEIAADCVRFLPGALDYARQGAIPGGLRNNMDF
                     ASCAVEITRELPQEVESLLYDPQTSGGLLIALPEEDAVKLANRFPQAYRIGRAIPRAA
                     KPICIV"
     gene            1211..1906
                     /gene="pyrF"
                     /locus_tag="VFQ79_06175"
     CDS             1211..1906
                     /gene="pyrF"
                     /locus_tag="VFQ79_06175"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU0139275.1"
                     /translation="MNNNPLIIALDFDAPEQALSLVDRLGNDIDFYKVGMELYAAAGM
                     DFVRNLLGAGKQVFLDLKLYDIGETVKRAVRQIATTGVRFVTVHGSGPVMRAAVEGRS
                     GSSLQLLAVSVLTSFDQEDLADLGYQCPVEDLVSLRVRKAMESGLDGVVCSPLEASRV
                     RELAGPGAILVTPGVRSEGAARGDQKRVATPAEALRDGANYLVIGRQVTRSSDPRSEV
                     ARIQAEISPLVRR"
     gene            2037..5621
                     /locus_tag="VFQ79_06180"
     CDS             2037..5621
                     /locus_tag="VFQ79_06180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0139276.1"
                     /translation="MTSVGGLVTDPSGAVVERALISLKNPAAGITRQTRSDEAGRYLF
                     AQVPPGNYEIGASASGFNDVVITGITLLVSSPGTVNIAFSSIGTVAETVSVTANTVQV
                     NTTDATIGNAFGASAITQLPLEGRNVVGLLALQPGVVFIGEEQMDSRNGAVNGGRSDQ
                     ANVTLDGIDVNDHQDRFAFTSVLRVTLDSVQEFRVTTSNASADRGRGSGAQIELVTRS
                     GTNDLHGSAYEYHRNTVTTANSFFNNSAQPSVERPKLIRNTFGVSLGGPARRNRLFYF
                     LNYEGRRDAREDSVERLVPTESFRTGTLKYIRTDNSIGELTPEDLRTRVDPAGIGVNA
                     EVLKLFQSYPLPNSFNSGDTLNTAGYRFKAPVGLRWNTYIARLDYNLTPNGTHGLFLR
                     GNLQNDRNTGLPQFPGDPPSTVDLTNSKGLAASYNAVFRPNLIGTFRYGFTRQGVENS
                     GLQTASAVRFRTLDDRYALTTAFTRITPVHHTTQDLTWVKGAHSVQFGGVQRWIRNSR
                     VNYASSFHFAQSRASWLENSGSNLERNVTDLASRFRTSYQDAAAAILGIISQGTANYN
                     YDLQGNVLPIGAPVSRRFANNEYELYVRDTWRASREWTITGGIRWSLMPPVYETNGMQ
                     IAMSPSAEAWFNERSALASSGRPQSEAARVRYVPADDAAGGDLYPFHKRNFGPHLAIA
                     YSPQSEGWKWLTGGPGQSVIRAGWGMYYDLFGQGLIRYFDSNSFGLSSSIQNASSTLT
                     ELTAPRFTGVFDLPSQLIPPAPPGGFPVEAPDVFAFGGTVDSRLRPPYTMEMNFSVAR
                     EFPGGFYVQAAYVGRLSRRSLVQSDIAAPTNLTDPKSGMSYFEAAGQLAQMGRQRVPV
                     EQVGAIPFWENLWPGAAGAGLTATQRVYQRFLAAEPDYTAAIQNLDIRCQPACSIYGP
                     YAMFNRQFSSMTAWRSIANGSYHAMQWTLRKRLSRGVQFDVNYTWSKSIDLASRAERD
                     AVNNLGIIPNPWDPGLRRAVSDYDMTHQFNANWIIELPFGRGKKWLHRNRGIRDAVAG
                     GWQWSGIWRQTSGLPISVSNGRIWPTNWQWQANATQVGPVPAQQTTKNAPAPVPGGFG
                     GPNVFADPAAALAAYDFTYPGGVGQRNGIRGDGYFTIDMGLAKRFYMPYSEKHSLQFR
                     WEVFNATNSVRFDVRNLSLSLGDQATFGRYFGVLTSPRVMQFGLRYEF"
     gene            5625..7283
                     /locus_tag="VFQ79_06185"
     CDS             5625..7283
                     /locus_tag="VFQ79_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0139277.1"
                     /translation="MNKSVLVLFSLLPVLSSAAEPQASAVATFESLGLYYNHAGGEAK
                     CPVRYRVAGGAEWREGYPLVYDQRERQYRGSLVGLTPDTLYNIRLEPGGAPVEIEART
                     LSEKFPVGKTTHLSGGTTDQTLYIREGGTAAGWHLVTPTPGTKFVSDVFNLSDYNVVV
                     EADYVILRGLDLRNAGIHGVLIREGVQHVAVEDSRITGWGRIGGARIWGVFHGSDSGI
                     YAENDVGHLVIQRNLIEHPRGGANDWESGHPVGPQGITLIDSRGGNIIRYNTIRSTDD
                     HGFNDGIGGGSNYSFKGSPNRDSDIYGNIVSHCWDDAIESEGANRNVRIWSNYIHHTF
                     VHIATAATSMGPLYIFRNVFGKSRVSHQDSSGGMMIKTGMNYLTIDGERVSTGLGHRF
                     IFHNTALQPGGGLDVFSSHELHNAVSRNNIFYARGRAYPRDRGAPPNDFEHDLTGGYL
                     GGGFVQSMFLTGNPLEWFLAPVMKSVKWGRVEYTRNGKNFAITDPVVEVANPALDTAV
                     RLPGFNNDYAGAAPDIGAFENGRPPLRFGRDMAPGFERAPWERY"
     gene            complement(7393..8745)
                     /locus_tag="VFQ79_06190"
     CDS             complement(7393..8745)
                     /locus_tag="VFQ79_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU0139278.1"
                     /translation="MPPRRRCLSALLAGLMMLPACAGRKPGDPLKPGYNVYSKEQDIQ
                     LGQEAAAQIKQQADIVKNSALQEYVNRIGQRLASSPAAEGYPYSFTVINEPGINAFAL
                     PGGPIFVHADLIEAADNEAQVAGVMAHEIAHVALRHGTNQASKSQLIQLPAVLAGAVI
                     GDGSLLGQLGQLGLGLGVNSILLRYSRDAETEADALGARLMAEAGYNPIEMARFFEKL
                     EAEGGSRAPQFLSSHPNPGNRMKAVEAEIQTLPQRQYGFSAGNFQQMKAAVTKLPEPK
                     RPQQQAAATPASAGPPAAPTGGFQNFQTDRFALAYPQGWETFGDRRSRVLTIAPRQGL
                     VQTGSGQVSLGYGAVLSYFTPNARSLGPATNELISQLRQANSSMRTTGQRQVNVGGNP
                     GLLTDMVSASPYGGTERDVVLTVMRPEGLFYMVFVSPEQSSAQLGDTFNQMLQSIRFN
                     "
     gene            8828..11443
                     /gene="glgP"
                     /locus_tag="VFQ79_06195"
     CDS             8828..11443
                     /gene="glgP"
                     /locus_tag="VFQ79_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU0139279.1"
                     /translation="MKPIQVFSVAPALPERLEPLGTLAQNLHWAWSHETIELFRRLDA
                     NLWESVNHNPVMLLGSIDQQKLENAAMDESFLAHMDGCLKDLDTCLNGDGTWFHRNHG
                     KDGDTPLVAYFSAEFGLTECLSIFAGGLGVLAGDHLKSSSDLGIPLAGVGLLYQQGYF
                     RQRLNARGWQEEEYESNDFHTLPLSPVLDPASGSPLAISVAFPGRSVFARVWKASVGR
                     VSLYLLDTNVPENSPADRDITDQLYGGGIEMRLEQEILLGIGGCRMLDALGLSPTVFH
                     MNEGHSAFLALEHVRTLMRAHGLSFSEARQVAFGSLIFTTHTAVPAGHDYFPPDLMER
                     YFREYRPELGLSLHDFLALGRQNPDDGNEHFCMTILALRMAAFRNGVSKLHGEVTRNM
                     WQGIWKNLPVDEIPIGHVTNGVHFRSWISRELNQLYDRYLGPRWRDEPGNAELWRRVE
                     SIPPEELWRTHERRRERLVAFARRRLKAQLQRRAAPESEIELSDEVLDTEALTIGFAR
                     RFATYKRATLLLRDVERLKQLLNNRERPVQLIFAGKAHPKDEPGKELIRQVVDLARDP
                     GIRNRLVFLEDYDMAVGRYMVQGVDIWLNNPLRPLEASGTSGMKAAANGALNASILDG
                     WWDEAWLSQQPGAEPIGWAIGTGERRPDAAEQDQAEAEALYNLLERDIVPMFYDYAGG
                     RLPRRWIARMKSSTSNLCYFFNTHRMVREYTERFYLMAHTNHRELVAGGGARARLLAQ
                     WGAQVQQAWPRVHLEIPNGRIAADLRVGGRIQVKVLVNLDVLTPEQVSVELYLGPLNP
                     QGEIVEPIAQQMSVSGRDARGWYIYETSAAPCCRSGQYGYTARVLPYHVNMIDGFMPG
                     LISWAVPGQANAASR"
     gene            11520..11864
                     /locus_tag="VFQ79_06200"
     CDS             11520..11864
                     /locus_tag="VFQ79_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU0139280.1"
                     /translation="MEHEHMEHESRFSYFFLGVGIGVAIGIVFAPKSGRDTRTLLKTK
                     ADESKEYLAKTGEQIRESATGLYERGKNVVSKQRDQVSAAVEAGKQAYHEAIRSSQTG
                     ESAPPAPDAGKL"
     gene            11917..12396
                     /locus_tag="VFQ79_06205"
     CDS             11917..12396
                     /locus_tag="VFQ79_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139281.1"
                     /translation="MTAAVVVSAVAIVVQLLILLGMYRSARGLQEQVTAFIPKAESLV
                     AAAQSTLEQSRNQISDITAKANDILDKTRVQVGRADEILSDATARAKVQMDRLELVFD
                     DTVGRLHETVAIIHNGILAPLREVNGIFSGIRAALGHLSRGGRPTVAQATQDDEMFI"
     gene            12462..>13168
                     /locus_tag="VFQ79_06210"
     CDS             12462..>13168
                     /locus_tag="VFQ79_06210"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEU0139282.1"
                     /translation="MIDIHSHILWNLDDGARDFDESVQMLRLAAVSGTSDIVATPHAN
                     LCYTFDLDAVNERIQRLSGKIDSIRIHRGCDFHFYTDNINDALANPSRYTINGRRYLL
                     VEFSDLLIFQNTGEMFDEMLAAQMVPVITHPERNPLLYSRTGDLAAWVGRGVCLQVTA
                     QSLLGRFGSDARDFAHELIERGLVAFLASDAHDCVDRPPRLDLGYRQLAQRYAERIAR
                     ALCIENPRAVLTGDPLP"
BASE COUNT         2609 a         3915 c         3977 g         2667 t
ORIGIN      
        1 tgagacaccg ccaaaattgc gtctatggga caaagagatg ctcatgccct cttgaatctc
       61 taatgaatta tgtgatccag ggccgcggga ctgtcaagaa gaaaatgcaa gtggattgca
      121 actgatgccg aaaggcacaa acaggggctc cagcggcttc ctaccccgcg gctccgctac
      181 actggtgggg aatggcctcc ggaaaactta ccgctcatgt gaaagcggga ggatgcgcgt
      241 cgaaattgag tccaaagatt ctggaccgcg tgctcgcgca gcttccacgc gtggcagatg
      301 agaacgtact ggtcggcttc gacaccgccg acgatgccgg ggtatataag ctcacgccgg
      361 aatgtgccct ggtccagacc gtcgattttt tcacgccggt cgtcgacgat ccgtttacgt
      421 ttggcgccgt cgcggcggcg aatgcgttga gcgacgttta cgccatggga ggccgcccgg
      481 tttccgccct gtcgattgtc gcgtatcccg ccaagggcga tatcgaggaa ctggagcaga
      541 tactgcgcgg gggtgccgag aagatgcgtg aagccggatg cgtcgttctc ggcggtcaca
      601 gtgtagccga cgatgagatc aagttcggct atgccgtcac cggcatcgta catcccgaac
      661 gcgtgaagac taacgccggc gcccgcgcgg gcgatgtgct tctgtttacc aagagcatcg
      721 gcatcggggt ggtttccacg gctctcaagc gcggcatcgc cgaccctgcc gatgtggaag
      781 cgtccatcgc atcgatgctg actcttaatc gagccgcctg cgaagccatg ctcgaagagg
      841 atgtacacgg ctgtaccgac gttacgggct tcggcctgat ggggcacgcc cgggagatgg
      901 ctatggcaag cgggctgaca ctggaaattg ccgccgattg tgtgcgattt cttcccggcg
      961 cgctcgacta cgcccggcag ggagcgatac caggtgggct cagaaacaac atggactttg
     1021 cctcctgcgc ggtcgaaatc acacgcgaac tgccgcagga ggtcgagagc ctgctctatg
     1081 atccacagac ttcaggtggc ttgctcatcg ccttgccgga ggaggacgcg gtgaaactgg
     1141 caaacagatt tccgcaagcc tatcgcatcg gccgggccat cccgcgcgcc gcaaaaccca
     1201 tttgcatcgt atgaacaata atcctctcat cattgcgctc gacttcgatg ctccggaaca
     1261 ggcgctctcg ctcgtggacc ggctgggcaa cgacatcgat ttttacaagg taggcatgga
     1321 actgtatgcc gccgcgggaa tggatttcgt gcgcaacttg ctcggcgccg gcaagcaggt
     1381 gttcctggac ctgaaacttt acgatatcgg cgagacggtg aaaagagccg tcagacaaat
     1441 cgccactacc ggtgtgcgct ttgtcactgt acacggcagc ggtccggtga tgcgcgcggc
     1501 cgtggagggg cgttccgggt cttcgctcca attgctggcg gtctccgtgc tgacaagctt
     1561 cgatcaggag gacctcgccg acctgggtta tcaatgcccg gtggaggatc tggtatcgct
     1621 gcgcgtgaga aaggcgatgg agtcgggcct cgacggcgtc gtctgctcgc cgttggaagc
     1681 ctcgcgcgtg cgggagttgg ccgggcccgg ggcgattctc gtcacgccgg gtgtgcgctc
     1741 cgagggcgcc gcccgtggag accagaagcg tgtagccaca cccgccgaag cccttcgaga
     1801 cggcgcgaat tacctggtca tcggacggca ggtgacgaga tcgagcgatc cgcggtccga
     1861 ggtggcccgg atccaggcgg agatcagccc gctggtccgg cgatagggtg tggcgagcct
     1921 accgtctgtt aagatgaacc gcaaggcgtt ttcggaacgg tgaagtgaac cgactgcttt
     1981 cagcttcgct tttgttttcg gcctcatcct gcttctgcgc gatgttcgcg caatcgatga
     2041 cttccgttgg cgggctggtt acagaccctt cgggcgcggt cgtcgagcgg gcgctcattt
     2101 cactcaaaaa tccggctgcc ggtatcacgc gccaaacccg ctcggatgaa gccgggcgat
     2161 acctgttcgc acaggtgcct cccggcaact atgaaattgg agcttccgcc tcgggattta
     2221 acgatgtcgt catcaccgga attacgctgc tggtaagttc tcccggaacc gttaatatcg
     2281 ccttttcctc tatcggcacg gtggccgaaa cggtatcggt cacggccaat accgtacagg
     2341 tgaacacgac ggatgccacc atcggcaacg ccttcggtgc gagtgccatc acgcaactcc
     2401 cgctggaggg ccgcaacgtc gtcggcctgc ttgcgcttca gccgggtgtc gtcttcatcg
     2461 gcgaagaaca gatggattcg cgcaacggcg cggtaaacgg cggcaggagc gatcaggcaa
     2521 atgtgacgct ggatggcatc gatgtaaatg accatcagga caggttcgcc ttcacgagcg
     2581 tgctgcgcgt gaccctcgac tcggtacagg agtttcgtgt caccacgagt aatgccagcg
     2641 cggaccgggg gcggggctcc ggcgcccaga tcgagcttgt caccaggagc ggtacgaacg
     2701 atctgcacgg ctcggcttat gaataccatc gcaacaccgt caccactgcc aattcgttct
     2761 tcaacaactc cgcgcagccg tccgtggaac ggccgaagct cattcgtaac accttcggcg
     2821 tgtcgctagg cggccctgcg cgcagaaatc ggctcttcta tttcctgaac tatgaaggcc
     2881 gccgcgacgc ccgcgaagac agcgtggagc ggttggttcc taccgaaagc tttcgcaccg
     2941 gcacgctgaa gtacatcagg acagataaca gcatcggcga gttgactccg gaagacctgc
     3001 ggacgcgcgt cgacccggct ggtatcggag taaacgcgga ggtgctgaag ttgtttcagt
     3061 cctatccgct gccgaactct ttcaactccg gagacacgct gaatacggct ggataccggt
     3121 tcaaagcccc tgtcgggctg cgctggaaca cctacatcgc gcgcctcgat tacaacttga
     3181 caccaaacgg tacgcacggc ctgttcctgc gcggcaacct tcagaacgac aggaatacgg
     3241 gcttgccgca gttcccgggc gacccgccgt caaccgtcga tttaacgaac agcaagggtc
     3301 tggccgcaag ctacaatgcc gtgtttcgcc cgaacctcat cgggactttc cgctacggat
     3361 tcacgcggca gggcgtagag aactcgggcc tgcaaacggc gtccgccgtg cggtttcgaa
     3421 cgctcgatga ccggtacgct cttacgacgg ccttcacgcg tattacccct gtccaccaca
     3481 cgacgcagga cctgacgtgg gtgaagggcg ctcacagtgt gcagttcggc ggggttcagc
     3541 gctggatccg caattcgcgg gtgaattacg ccagttcttt tcatttcgcc cagtccaggg
     3601 catcgtggct cgaaaactcg ggttcgaatc tcgaacgcaa tgtcaccgat ctggctagcc
     3661 gtttccggac atcgtatcag gacgccgctg cggcgattct cggcattatt tcgcaaggta
     3721 ccgcgaatta caattacgat cttcagggca acgtgttgcc catcggcgca ccggtttcca
     3781 ggcggttcgc caacaacgaa tacgagttgt acgtgaggga cacctggaga gccagccgcg
     3841 agtggacgat caccggaggg attcgctggt ccttgatgcc tccggtatac gagaccaatg
     3901 gaatgcaaat cgccatgtcg ccctccgcgg aggcatggtt caacgaacgg agcgcgctgg
     3961 cttcgagcgg aaggccgcaa tcggaagcgg ctcgcgtgcg ctatgttccg gcggacgatg
     4021 cggcgggcgg agacctttat ccatttcaca agagaaattt cgggccgcat ctggccattg
     4081 cctattcccc gcaatcggaa ggttggaagt ggctcaccgg cggtccgggc cagagcgtga
     4141 ttcgtgccgg atggggcatg tattacgatc tcttcggcca gggtttaatc cgctatttcg
     4201 acagcaactc gttcggcctc tcgtcttcta tccagaatgc gtcgagcacg ttgaccgaac
     4261 tcacggcacc gcgtttcacc ggcgtattcg atcttccatc gcagctcatc cctcccgcgc
     4321 ctccgggtgg ctttcccgtg gaagcacccg atgtgttcgc tttcggcggc acggtggaca
     4381 gccgcctgcg cccgccctac acgatggaaa tgaacttctc ggtggcgcgc gagttcccgg
     4441 gcggcttcta tgttcaggcg gcgtatgtgg ggcgtctctc gcgccgctcc ctggtgcaga
     4501 gcgacatcgc cgctcccacg aacctgaccg atccgaaatc cggcatgagc tactttgagg
     4561 cagccggaca actggcccag atgggccgcc agcgcgtacc tgtcgaacag gtgggggcga
     4621 ttccgttctg ggagaacctc tggcccggcg cggccggtgc cggcctgacc gccacgcagc
     4681 gtgtttacca gcggttcctg gcggccgagc cggattacac cgccgcgatc cagaatctcg
     4741 acattcgttg tcagcctgca tgtagcatct acgggcctta tgcgatgttc aaccggcagt
     4801 tttcgtccat gaccgcatgg cgctccattg cgaacggcag ctatcatgcg atgcagtgga
     4861 cgctacgcaa gcgcctgagc cggggcgtgc agttcgacgt gaactacacg tggtcaaaat
     4921 cgatcgatct tgcctcgcgg gccgagcgcg atgccgtaaa caacctgggc atcattccca
     4981 acccctggga ccccggactc cgccgcgctg tgtccgatta cgacatgacg caccagttca
     5041 atgcgaactg gatcattgag cttccttttg gccgcggtaa gaagtggctg cacaggaaca
     5101 ggggaatccg cgatgcggtt gccggcggtt ggcagtggag cggaatctgg cgccagacca
     5161 gcggtcttcc gatcagtgtc agcaacggcc gtatctggcc cacaaactgg caatggcagg
     5221 cgaatgcgac gcaggtgggc ccggtgcccg cgcagcagac gacaaaaaac gcaccggcgc
     5281 ccgtgcccgg cggcttcgga gggccgaacg tgtttgcgga tccggccgcg gctctcgccg
     5341 cttatgattt cacgtatccc ggcggcgtag gacagaggaa cgggattcgc ggcgacggct
     5401 atttcaccat cgacatgggt ctggcgaagc gtttctacat gccctacagc gagaagcata
     5461 gtcttcaatt ccgttgggaa gtcttcaacg ccaccaattc cgtccgtttc gacgtgcgca
     5521 atctgtcgct cagcctcggc gaccaggcga cgttcggcag gtattttggt gtcctgacca
     5581 gcccgcgagt aatgcagttt gggttacgct acgaattctg agaaatgaat aaatccgtcc
     5641 ttgttctttt cagcctgttg cctgttctct ccagcgccgc cgaaccgcag gcgtcggccg
     5701 ttgcaacatt tgaatcgctg ggcctgtact acaaccatgc gggtggggaa gcgaagtgtc
     5761 cggtgcggta tcgcgttgcc ggtggtgcag aatggcgcga aggatatccc ctcgtctacg
     5821 accagcgtga gcgccagtac cgcggctcgc tcgtgggtct cacgccggat acgctctata
     5881 acatccggct ggagccaggc ggtgctcccg tggaaatcga agcccggacc cttagcgaaa
     5941 aattccctgt tggaaaaacc acgcacctca gcggggggac caccgatcag actctctaca
     6001 tacgcgaggg cggcactgcc gccggatggc acctggtcac gccaaccccc ggcacgaagt
     6061 tcgtgagcga tgtgttcaac ctgtccgact acaacgtggt ggtcgaggcg gactatgtca
     6121 tccttcgcgg tcttgatctg aggaacgcgg ggattcacgg cgtgctcatt cgggagggcg
     6181 tgcagcacgt tgccgtcgag gattcacgga tcaccggatg gggaagaatc ggcggcgcgc
     6241 gcatctgggg tgtcttccac gggtcggata gcggcatcta cgccgaaaac gatgtgggcc
     6301 atctggtgat ccaacgcaac ctgatcgaac atcctcgcgg cggcgctaac gattgggagt
     6361 cggggcaccc ggttggacca caagggataa cgcttatcga ttcgcgaggc ggcaatatca
     6421 tccgctacaa caccatccgc tcgacggacg atcacgggtt caacgacggc atcggcggcg
     6481 ggtcgaatta cagcttcaag ggaagcccga atcgcgattc ggatatttac ggcaacatcg
     6541 tctcgcactg ttgggatgat gccatcgaga gcgaaggcgc aaaccggaat gtgcggatct
     6601 ggagcaacta cattcaccac acgttcgtac atatcgcgac cgcagccacc tccatgggcc
     6661 cgctctatat tttccggaat gtgttcggga agagccgcgt ttcacaccag gattcttccg
     6721 gcggaatgat gatcaagacg ggtatgaact acctgaccat cgacggcgag cgcgtcagca
     6781 cgggcctcgg ccaccgcttt atcttccata acacggcgtt gcagccgggt ggcggcctcg
     6841 acgtattcag tagtcatgaa ctgcacaacg cggtcagccg gaacaatatc ttctacgcgc
     6901 gtgggcgcgc ctatccacgg gaccgcggcg caccgcccaa cgacttcgag cacgatctga
     6961 ccggcgggta tcttggcggt ggatttgtcc agtcgatgtt tctcacaggc aacccgctgg
     7021 agtggttcct ggcgcccgta atgaagagcg tcaagtgggg cagggtagag tatacgcgga
     7081 acggaaaaaa cttcgccatt accgatccgg tggtcgaggt ggcgaatccg gcactcgaca
     7141 ccgccgtacg cctaccggga ttcaacaacg attacgccgg ggccgcgccc gacatcggag
     7201 cctttgaaaa cgggcgtccg ccgctgcgct tcggccgcga catggcgcca gggttcgaac
     7261 gcgctccgtg ggagcggtat taaacatggc gtcagaaatg actgcggtcg cggctcggca
     7321 ttcgtttccg agccacggcc gccagggagt ggttatcggg atacgcaaac agaagcggcg
     7381 tcgaagcttc cctcaattga aacggatcga ttggagcatc tggttgaatg tgtctccgag
     7441 ctgggccgac gattgctccg gcgacacgaa taccatgtaa aagagccctt ccggccgcat
     7501 cacggtcaac accacgtccc gctcggttcc accgtaaggg gacgcgctga ccatgtcggt
     7561 aagcaacccc ggattcccgc ctacgttgac ctgccgctgc cctgtcgtcc gcatgctgct
     7621 gtttgcctgc cgcaactggg agatcaattc gttggttgcc ggacccaggc ttctggcgtt
     7681 cggagtgaaa tagctcaaca ccgcaccgta gcccaggctt acttgtccgg atcctgtttg
     7741 caccagcccc tgccgtgggg caattgtaag cacgcgcgac cgcctgtcgc cgaaagtctc
     7801 ccaaccttgc ggataggcga gtgcgaaccg gtccgtctgg aagttctgaa aaccgccggt
     7861 gggcgcagcg ggtggcccgg cggaagcagg ggtagcggct gcctgctgtt gcggccgctt
     7921 tggttcgggc agcttcgtga cggcggcctt catctgctga aagtttcctg cgctgaagcc
     7981 gtactgccgc tgcggcagcg tttggatctc cgcctctact gctttcatcc ggttgccggg
     8041 attgggatgg gaggagagga actgcggcgc gcgcgagcct ccctcggctt cgagcttctc
     8101 gaagaaccgc gccatctcaa tagggttgta gccggcttct gccatcagcc gggctcccag
     8161 tgcgtccgct tcggtttccg cgtcgcgcga ataccgcagc aaaatggaat tgacgcccag
     8221 tcctagcccc agttggccca gttgccccag aagggacccg tctccgatga ccgcccccgc
     8281 cagcaccgcg ggaagttgaa tcaattgcga tttcgatgcc tgattcgttc cgtgacgcaa
     8341 agcgacatgc gcgatctcgt gtgccatcac gcccgcgacc tgcgcctcat tatcggcggc
     8401 ttcgatcaga tcggcgtgca cgaagatcgg accgcccggc aaggcaaagg cgttgattcc
     8461 gggttcgttg attaccgtga aagagtacgg atatccctcc gccgcgggtg atgacgccag
     8521 cctctgtccg atgcgattca catattcctg aagcgcggaa tttttaacga tgtcggcctg
     8581 ttgcttgatt tgcgcggctg cttcctggcc cagttgaatg tcctgctctt tggagtacac
     8641 gttatagccc ggcttgagcg ggtcaccagg cttcctgccc gcgcaggccg gtagcatcat
     8701 cagtccggcc agcagtgcgg acagacacct tcttctcggt ggcattcact ttccccttgc
     8761 aatcagagac tccggacaag cggcaggtgt ttcagccgaa attcaggtgt ataaaggagt
     8821 taaccgtatg aaaccgattc aggtcttttc cgtggcccca gcccttcccg agaggctcga
     8881 acctctgggt accctggcac agaatcttca ctgggcatgg agccatgaaa ccatcgagct
     8941 gttccgccgc ctcgacgcca atctctggga atcggtaaat cacaacccgg taatgctgct
     9001 cggaagcatt gaccagcaga agctggagaa tgccgcgatg gacgagagct ttctcgccca
     9061 catggacggg tgcctgaagg atttggatac ctgtctgaat ggcgacggaa cctggtttca
     9121 tcgaaaccat ggcaaggacg gggatacacc gctggtggcc tacttctccg cggaattcgg
     9181 actcacggaa tgcctgtcga tattcgccgg cggccttggt gtgctggctg gggaccattt
     9241 gaaatcgtcg agtgacctcg gcattccgct tgccggcgta gggctcctgt atcagcaggg
     9301 ctatttccgc cagcgcctga acgcacgcgg atggcaggaa gaagagtacg agtccaacga
     9361 ttttcacacc ttgccgcttt cgcccgtact cgatccggca agcggttcgc cgctcgcgat
     9421 ctccgtcgca tttccgggcc gcagcgtctt cgcccgggtt tggaaagcat cggtgggccg
     9481 cgtctcgctt tatctgctgg acaccaacgt tcccgagaac agtccagcgg atcgcgatat
     9541 caccgatcag ctctatggcg gcggcattga aatgcgcctc gaacaggaga tcctgctggg
     9601 tatcggcggt tgccggatgc tcgatgctct cggcttatcg cccaccgtgt ttcacatgaa
     9661 cgagggccac tcggcgtttc tcgcgctgga gcatgtcagg actctcatgc gcgcgcatgg
     9721 gctctcattt tccgaagcgc ggcaggtggc ttttggcagt ctcattttta cgactcacac
     9781 ggcggttccc gcgggtcacg actattttcc cccggacctg atggagcgct acttccggga
     9841 gtaccggccc gaactcgggc tatcgttgca cgactttctc gctctcggac gccagaaccc
     9901 cgacgatgga aatgagcatt tctgcatgac catactggcg cttcgaatgg ccgcgttccg
     9961 caacggcgtc agcaaactgc atggcgaggt cacgcggaac atgtggcaag gcatttggaa
    10021 aaaccttccc gtggacgaga ttcccatcgg acatgtcacc aatggagttc acttccgttc
    10081 gtggatttcc cgggagctga accagctcta cgaccgctat ctcggtccga ggtggcgcga
    10141 cgagcccggc aacgccgagt tgtggcggcg cgtggaatcc ataccgcccg aggagctgtg
    10201 gcggacgcac gagaggcgcc gcgagcgcct ggtggctttt gcacggcgcc ggttgaaagc
    10261 gcagctccag cggcgcgcgg cccccgaatc ggagatcgag ttatccgacg aagttctgga
    10321 caccgaggcg cttaccatcg ggttcgcccg gcggtttgcc acctacaaac gggcgacact
    10381 gctgctgcgc gatgtcgagc ggctgaagca gcttttgaac aatcgcgaac gtcccgtcca
    10441 gcttatcttt gccggcaagg cgcacccgaa agatgagccg ggcaaggagt tgatacgcca
    10501 ggtggtggat ctggccaggg accccgggat ccgtaaccgc ctcgtatttc tggaagacta
    10561 cgacatggcc gttggcagat atatggtcca gggagtggat atctggttga acaatcccct
    10621 gcggccgctc gaagccagcg gcaccagcgg tatgaaggcg gcagccaatg gagcgctcaa
    10681 tgccagtatt ctggacggct ggtgggacga ggcttggttg tcgcagcagc cgggcgccga
    10741 gcccatcggg tgggcgattg gcaccggaga acgccgtccc gacgccgccg aacaggacca
    10801 ggccgaagcg gaagctcttt ataacctgct cgaacgcgat attgtcccga tgttctatga
    10861 ctacgccggt ggacgcctgc cacgccgctg gatcgcgcga atgaaatctt ccacctccaa
    10921 cctgtgctac ttcttcaata cgcaccggat ggttcgggaa tacaccgagc ggttctacct
    10981 gatggcccac acgaaccatc gggaactggt tgccggcggc ggcgcccgag cccggctgtt
    11041 ggctcagtgg ggggcgcagg tccaacaagc ctggccgcgc gttcatctcg aaattcccaa
    11101 cgggcgcatt gccgcggacc tgcgtgtggg cggccgtatt caagtgaagg tgctggtgaa
    11161 cctcgacgtg cttactcccg agcaggtctc agtcgaatta tatctgggcc cgctcaatcc
    11221 acagggagaa atcgtggagc ccatcgcgca acagatgtct gtttcgggca gggatgcacg
    11281 tggctggtat atttacgaaa cctccgccgc tccctgctgc cgtagcggtc agtatggcta
    11341 taccgcgcgt gtgcttccat atcacgtcaa catgatcgac gggttcatgc ccggcctcat
    11401 ctcgtgggcc gttcccggcc aggcaaacgc cgcttctcgc tgaccgttta caaacgtcgg
    11461 tatcgccatc cctgctagta tttgagtgag agtgcacgaa ctcggaaagg aacacgctta
    11521 tggagcacga gcacatggag cacgaaagca gattttcgta cttcttttta ggagttggca
    11581 tcggagtagc gatcggaatc gtgttcgccc cgaaatcggg ccgtgacaca cggactctgc
    11641 tgaagacgaa ggcggatgaa agcaaagagt atctcgcaaa gaccggagaa caaatccgcg
    11701 agtccgccac tgggctgtat gaacgcggaa aaaatgtcgt aagcaagcag cgggatcagg
    11761 tttccgcggc tgtggaagca ggaaaacagg cgtatcacga ggcgattcgt tcctcgcaaa
    11821 cgggcgaatc ggctccaccg gcgcccgatg cgggaaaact ctgatcaaaa gattttcagg
    11881 tactagaccg tgattccaga taccgtcatc tatgttatga cggcagcggt agttgtttcc
    11941 gccgtcgcca ttgtggtgca gttgcttatt cttttgggaa tgtaccgctc cgccagaggt
    12001 ctacaggagc aggtaacggc ctttatcccg aaagcggaat cgctggtggc ggcggctcaa
    12061 agtactcttg aacagagccg gaatcagatt tccgatataa ccgcgaaagc caacgatatt
    12121 ctggacaaga cccgggttca ggtcggccgt gcagatgaaa tactcagcga cgccaccgcc
    12181 cgggccaaag tccagatgga ccgcctggag ctggttttcg acgataccgt cggacgtctg
    12241 cacgagacgg tggcaattat tcacaacggc atcctggcgc ctcttagaga agtaaacggg
    12301 atcttttcgg gaatccgcgc ggcgcttggg catttatccc gcggggggag gcccaccgtt
    12361 gcgcaggcaa cgcaggacga tgagatgttc atttaagcta tgtacgggtc cgcttttgtc
    12421 gtacaggccg gcgccgccgc gcgtcgctgc ttgccggtcg catgatcgat atacatagcc
    12481 acatcctctg gaatctggat gatggcgccc gggacttcga tgaaagtgtt caaatgctgc
    12541 gcctcgcggc ggtctccggg acttcggata tcgttgctac gccccacgcg aatctgtgtt
    12601 acacgtttga tcttgacgct gtaaacgaac gcatccaacg tttatccggc aaaatagact
    12661 ccattcgaat tcatcgcggc tgcgatttcc acttctacac cgacaacatc aacgacgcgc
    12721 tcgcaaatcc ctcccgctac accattaacg gccggcgtta tctgttggtc gaattttccg
    12781 atcttctgat tttccagaac accggagaaa tgttcgacga gatgcttgcg gcgcaaatgg
    12841 tgcccgttat tacgcatccg gaacgaaacc cgctgctcta tagcaggacg ggcgacctcg
    12901 ccgcgtgggt ggggcgcgga gtttgtttgc aggttacggc gcagtcgctg ttgggacgct
    12961 tcggaagcga tgccagggat ttcgcgcatg agttgattga acgcggattg gtcgcatttc
    13021 tggcaagcga cgctcacgac tgcgtcgaca ggcccccccg gttggactta ggctacagac
    13081 aactcgcgca gcgctatgcc gaacgaatcg cccgcgctct ctgcatagaa aaccctcgcg
    13141 ccgttctgac aggcgaccct ttgcccat
//
LOCUS       DASTMK010000148         6546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_583839, whole genome shotgun sequence.
ACCESSION   DASTMK010000148 DASTMK010000000
VERSION     DASTMK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583839"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFQ79_06215"
     CDS             <1..303
                     /locus_tag="VFQ79_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0139283.1"
                     /translation="FPAALTNFRKALDGDSTDPDYHFNIGYVLWKEGNFSDAAESFRA
                     VLERDSEDSEAKTMLSRCLKASGPRPGDPNADLERLKEDFNETVYLQLIEALGPKS"
     gene            350..2962
                     /locus_tag="VFQ79_06220"
     CDS             350..2962
                     /locus_tag="VFQ79_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139284.1"
                     /translation="MIFSFRASPVFTFLSLCMGLAAQTVAPAGSGPAAVRTPPGKPAQ
                     NAARPSIGASAQRNENVAIYQIDNNAVKESNIRLGSRATVVTDAPVEASYYAAEHGQS
                     ASEPVFLAPVPAPASWHGELYHSHQNSVFNARTFFQAGPVMPSRRNFYGGRFTSTVPV
                     LGALTVTADQRKVRGMVNGNVLVPLASERQPLTADPRLRQIIAGFLNAYPNQLPNRPD
                     FDPRALNTNAPQTSDDTDLGLRIDRPFANKNRISASHVLTRRRQYAFQLVAGQNPDTE
                     IHTHRSRLTWERIFSEGTQLNLGFSFNRTRSLLLSEPNAVGPRVRFGYQIEELGPDSQ
                     FPINRAQNAYRWGALFRRQPAGSSHLFTIGGDVTRYQLNGVEANNQRGYFQFTNNFGR
                     TAIQNLLVGTPSLYEVTIGDLYRAFRSSSANLFFADKWSVSQRFQLYFGLRYSLEGAP
                     SEVRRASVIPYGCDCNNFSPRFSLAYRATDHWTARAAYNVSFGQIPPVTYQQVRNNLP
                     LVRYVQVQNPDLIEPLRNVDPNSGRTSPTLLAPDLVSPYAHQYSFTLERPVGKSVALR
                     LGYLGSRSLKLINVYITNRAVPVEGIPLTTATVDQRRPDPRFYEVNNIVNGGIAYFDA
                     GQLTVTSALRSGLAWSATYTFGKAIDEGSDYTFTAANRDLTTGRSQWQYDSLKDKKGL
                     STFDSTHSLTVAYSYELPSPAAHTAIGRAVLGSWQISGMTLFKTGTPLTLFIGSDGPG
                     YGNVDGGPSDRPNILDPSILGMTISNPDVAPRIITRDRFVYITPGENRGSLGRNTFRK
                     AGIANLNAALTKELRWRGAREWAALLRIEAYNLANHPQFDEPQRNLSSPAFGKITNTL
                     NDGRVLQIGLRIVL"
     gene            3033..3740
                     /locus_tag="VFQ79_06225"
     CDS             3033..3740
                     /locus_tag="VFQ79_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEU0139285.1"
                     /translation="MLEAFGLSDPGCVRANNEDYYLLAPSLGLYIVADGMGGANAGEH
                     ASKLAAQTVADFVWQAEEPCAQVLMKAFEEANRKVISAASNDSMLEGMGTTLVAALEC
                     GDDILIASVGDSRAYVYHAGQLLVVTEDQTWVHEVGRRLGIDEASLRTHPMRHVLTMA
                     IGVSEQLRVHSYAVKPQPGANFLLCSDGLHGVLDEDFIAETLSSDASLEAKCERLVHA
                     ARQNGGPDNITTVLLRS"
     gene            3838..4479
                     /locus_tag="VFQ79_06230"
     CDS             3838..4479
                     /locus_tag="VFQ79_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139286.1"
                     /translation="MKPRRIAALAVAALLLNGQTPAAAAESPSEIAILQMKVMEGDGA
                     IHAVSSRSTGRLVVTVTDETGKPVEAAAVSFRLPDEGPGGAFRSGLRNEILLTGPDGR
                     AAVGGIQWNDIPGPVRIRITAVKDKVRAGIVVDQYLSDQIPARSGVSRSGRDAHPWKR
                     GKWLAIALIAGGAAAAGVALGARGNGSGGGGPAGTSNTPVQIGAPTISVGGPR"
     gene            4476..5234
                     /locus_tag="VFQ79_06235"
     CDS             4476..5234
                     /locus_tag="VFQ79_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139287.1"
                     /translation="MRKTFLLLAMGACLAGGQSLDFPTLGYILAGDRVAHLFGVAGAF
                     SIGEPQYNDVLSAAFSGQAGLLKTAGEVLVLDSKGRTTASVAAPEGPALFAFSAQGSP
                     GGALFPAAGEIYLFRDEEVTRIAAELPAGEPVALGVSDSGTVTVLLDRDDGLWKSRFS
                     IANGQFETAEFVPEIQAPAVFLPGGSILGTTEEGLVVFFPEKARQQIPLHFRPSAIER
                     IGSGWLLLQADSRARYGIRLDQPEHLYELPGGPQ"
     gene            5231..>6546
                     /locus_tag="VFQ79_06240"
     CDS             5231..>6546
                     /locus_tag="VFQ79_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEU0139288.1"
                     /translation="MTIRLLALLACLPFVGAAQLRVFVVDGEAERELTAQFDMGKTDP
                     GDAIETRFRVRNSGVSAGTVQILTLGGAGFSFSNRPSIPYIIAPGFFMDFRVKFEPTG
                     PGSYSASLTVNSITAILRASSPPVLTLTAEGVVLTTSSPLNFGKVELGSSAARRIVIA
                     NNTSGALTLSTVALSGEAFELETVLTLPRVLQPRTAVNLETIFTPKVVGSFEGKLLVN
                     DRLFTLKGSAASPPVPRATISISPAVTSSGKQPKVSVELASPAAFAVTGVLTLGFQAN
                     ASHSDPTILFVRGGSKSIGFTVAQGETRATFPGGETETVLQTGTTAGTITLQIVSNSD
                     TLSIAIPASPVVVDMARGTRTASGLDIELTGFDNTRSVSQLRFQFFDRNGRAIGSGPV
                     SVDAGADFRSFFDASALGGVFSLRASFPVSGDASQIAGAEVELVNSA"
BASE COUNT         1291 a         2054 c         1897 g         1304 t
ORIGIN      
        1 ttccccgcgg cgctgaccaa ctttcgaaaa gcgctggacg gcgattccac cgatcccgat
       61 taccacttca acatcggcta cgtgctctgg aaagaaggca acttctccga cgcggcggaa
      121 agctttcgag ctgttctcga acgcgattcg gaagatagcg aagccaagac catgctgtcc
      181 cgctgcctga aggccagcgg tccccggccg ggcgacccca atgcagacct cgagcggttg
      241 aaagaggact tcaacgagac cgtgtacctc caactgatcg aagcactggg cccgaaatcc
      301 tgaaacggtg ccggcaccga acgaattaca ccgatacaat agcgagttca tgatcttttc
      361 tttccgtgcc agtcctgtct tcacattctt gtcgctctgc atgggattgg cggctcaaac
      421 cgtggcgccc gccggttccg gaccggccgc cgttcgaact ccacccggaa aacctgcgca
      481 gaacgccgcc cgcccaagca tcggcgcctc cgcacagcgc aatgaaaacg tggcgattta
      541 ccagatcgac aacaacgccg ttaaggaatc gaacatccgg cttggcagcc gcgcaacggt
      601 cgtgacagac gcgccggtcg aggcaagcta ctacgccgcg gagcatggac agagcgcgag
      661 cgaaccggta ttcctcgcac cggttccagc ccccgcgagc tggcacggcg agttgtacca
      721 ttcgcatcaa aacagcgttt tcaatgcacg aaccttcttc caggccggtc cggtgatgcc
      781 gtcccggcgg aacttctacg gcggccggtt tacatccaca gtccccgtcc tgggtgcctt
      841 gaccgtcacc gcagatcagc gcaaagttcg cggcatggtg aatggaaacg tgctggtccc
      901 tctggcctct gaacggcaac ccctgacggc agacccgcgg ctccggcaga ttatcgccgg
      961 ctttctgaac gcctatccca atcaactccc caaccggccc gatttcgacc cgcgcgcgct
     1021 caacacaaac gcgccgcaaa ccagcgatga cacggaccta ggcctgagaa tagaccgccc
     1081 tttcgcaaac aagaaccgga tctccgcttc tcatgtcctt acccgccggc ggcagtacgc
     1141 cttccaactt gttgcgggcc aaaatcccga cactgaaatc cacacccacc ggtcgcggct
     1201 gacctgggag cgcatctttt ccgagggcac acagttgaac ctgggcttct cgttcaaccg
     1261 cacccgttct ctcctgcttt ccgaaccgaa tgccgtgggt ccgagagtgc gattcggtta
     1321 tcagattgag gaacttggcc ccgacagcca gttccccatc aaccgggcgc aaaacgctta
     1381 ccgctggggc gctcttttcc gccggcagcc ggcaggatcg tcacacctct tcaccattgg
     1441 cggcgatgtc acccgctatc aactaaatgg cgtggaggcc aataaccagc gcggctattt
     1501 ccagttcacg aacaacttcg gccggacggc gattcagaat ctgctggtgg gtacaccttc
     1561 tctctacgag gtcacgatcg gagatttgta ccgtgcgttc cggagttcct ccgcaaatct
     1621 gttcttcgcg gataaatggt cggtctcgca acgatttcag ctttacttcg gcctccggta
     1681 ctctctcgaa ggcgcccctt cggaggtgcg gcgtgccagc gtcatcccct acggctgcga
     1741 ttgcaacaac ttcagcccga gattctcgct ggcttaccgc gccacagatc attggaccgc
     1801 gcgagccgct tacaacgtgt cgttcggaca gattccaccg gtcacctacc agcaggttcg
     1861 gaataacctg cccctggtgc ggtatgtgca ggttcagaac cccgatttaa tcgagccttt
     1921 gcgcaacgtc gatccgaatt ccggccggac ctcgccgacc ctgctcgcac cggacctggt
     1981 gtccccctac gcgcaccagt acagcttcac tctggaacgt ccggtgggca agagtgtcgc
     2041 cttgcggctc gggtatctgg gcagcagaag cctgaagctg atcaacgtct acatcaccaa
     2101 tcgggcggtt ccggtggaag gcattccgct gacgaccgcc acggttgacc agcgccgtcc
     2161 cgacccgcgc ttctatgagg tcaacaacat cgtcaatggt ggaatcgcct acttcgatgc
     2221 gggccaactg accgtcacgt ccgctcttcg gagcggcctg gcctggagcg ccacctacac
     2281 ttttggcaag gcaatcgacg aaggctcaga ctatacattc accgcggcca atcgggacct
     2341 gaccacggga aggagccaat ggcaatacga ttccttaaag gataagaaag gcctgagcac
     2401 cttcgattca acacactcgc tgacggttgc ttactcgtac gaattaccca gcccggcagc
     2461 tcacacggcc atcgggagag cggtgctggg aagctggcag atttccggta tgacactatt
     2521 caaaaccggc acccccctga cgctgtttat cggctccgat gggcccggtt acggcaacgt
     2581 cgacggcggg ccgagcgacc ggcccaatat tctcgaccct agtattctcg gaatgacgat
     2641 ctcaaacccg gacgtcgcgc cgcggatcat taccagggac cggttcgtct acatcacgcc
     2701 gggcgaaaac cggggctcgc ttggacggaa cactttccga aaggcgggaa tcgcgaatct
     2761 taacgctgcc ctgacgaaag aactgcggtg gcgcggtgcg cgtgaatggg cggcgctgct
     2821 cagaattgaa gcctacaacc tggcgaacca tcctcaattc gacgagccgc agcgcaatct
     2881 ttcatcgccc gcgttcggca agattacgaa tacattgaac gatggcagag tgctgcaaat
     2941 tggattgcgg attgtattgt gaaatagtac ctgagccttg gtactgcgcg ttgccgcgac
     3001 aaatggtaga ggggctataa tcggaacacc gcatgctgga agcgtttggc ttgtccgatc
     3061 ccggctgtgt gcgggctaat aacgaggact attacctact cgctccttcg ctcggcctct
     3121 atattgttgc agatggcatg ggcggagcca atgcgggcga acatgcctcg aagctcgccg
     3181 cgcaaaccgt cgccgatttc gtgtggcagg ccgaagagcc ttgtgcccag gttttgatga
     3241 aggctttcga agaggccaac cgaaaggtga tatcggctgc cagcaacgat tccatgcttg
     3301 aaggcatggg aactacgctg gtcgcggcgc tggaatgcgg cgatgatatc ctgatcgcca
     3361 gcgttggcga tagccgcgcc tacgtctacc acgccggcca gttgctggtc gtcaccgaag
     3421 atcaaacctg ggtgcatgaa gtcggcagac gcctgggtat cgacgaagca agccttcgca
     3481 cccaccccat gagacacgtg ctcaccatgg ccatcggagt cagcgagcag ttgcgcgtac
     3541 attcctatgc cgtaaaaccg caaccgggtg caaatttcct gttgtgcagc gacgggttgc
     3601 acggcgtcct ggacgaggat ttcattgctg aaacgctcag cagcgatgcc tcgctggaag
     3661 ccaagtgcga gcggctcgtg cacgctgccc ggcagaacgg cggcccggac aacatcacca
     3721 ccgttctttt acgctcctga tcttccctcc cgcgccgcgg aattcgcatt ttacttaggc
     3781 cagtactacc ccaatggaga taatcccaac ccgcccgcca ctattctttt tcaggctatg
     3841 aaaccgcgca gaattgccgc tttggcagta gccgcgctgc tcctcaacgg gcagactccc
     3901 gcggccgctg ccgaatcgcc ttccgaaatt gccattctcc agatgaaggt gatggaaggc
     3961 gacggcgcta tacacgctgt aagctcccgt tcgacagggc gccttgtcgt aacggtgacc
     4021 gacgagacgg gcaagcccgt cgaggccgcc gccgtcagct tccgcttgcc cgatgagggg
     4081 cccggcggcg cattccggag cggcctgcga aacgaaattc tgttgaccgg gcccgacggg
     4141 agagcggcgg tgggaggcat ccagtggaac gacatcccgg ggccggttcg catccgaata
     4201 acggccgtca aggataaggt tcgggcggga atcgttgtgg atcaatatct ttccgaccag
     4261 atcccggccc gaagcggcgt ttcccgaagc ggaagagacg cgcatccttg gaagcgcggg
     4321 aaatggctcg ccattgccct tatcgccgga ggtgccgctg cggcaggggt cgcgctgggc
     4381 gcccgcggca atggctccgg cggcggtgga ccggctggca cctcgaacac cccagtccag
     4441 atcggcgcac ccaccatcag tgtcgggggc cctcgatgag aaagacgttt cttttgttgg
     4501 ccatgggagc atgtctggcg ggcggtcagt cgctggattt tccgacgctc ggctacatct
     4561 tggcaggcga tcgggtggcg cacctgtttg gggtggcagg ggcgttttct attggagagc
     4621 cgcaatacaa cgacgtgctc tcggctgcgt tttcaggcca ggcggggctc cttaaaaccg
     4681 ccggtgaagt gctggtgctc gattcgaaag gcaggacgac tgcttccgtg gccgcgccgg
     4741 aaggcccggc cttgtttgct ttcagcgcac agggatcccc cggcggtgcc ctcttcccgg
     4801 cggcaggtga gatctatctg tttcgagacg aagaagttac acgcatcgcc gccgaacttc
     4861 cggcgggtga gccggtggcg cttggtgttt ccgattccgg cacggtaacg gttctgctgg
     4921 accgcgacga cgggctctgg aaatcgcgtt tttccatcgc caacggtcaa ttcgagaccg
     4981 ccgaattcgt tccggaaatc caagcacccg cggtgttcct gcccgggggc agcatcctcg
     5041 gtacgaccga ggaggggctt gttgtttttt tccccgaaaa agctagacaa caaattccat
     5101 tgcatttccg cccttccgcc atcgaacgga ttggtagcgg ctggttgctg cttcaagccg
     5161 atagccgagc gcgttacggc atccggctcg atcagccgga gcacctgtat gagttgccgg
     5221 gaggccccca atgaccatcc ggctgctcgc cctgttagca tgcctgccgt tcgttggagc
     5281 ggctcaactc cgcgtattcg tggtagatgg ggaggccgaa cgcgaattga cggcgcagtt
     5341 cgatatggga aaaaccgatc cgggcgacgc gattgaaacg cgattccggg tgcggaattc
     5401 gggcgtgagt gccgggacgg tgcagattct taccctcggc ggcgccggat tctccttttc
     5461 gaaccggccc tccatcccct atatcatcgc cccggggttc tttatggact tccgggtgaa
     5521 gttcgagcca accggccccg gctcttacag cgccagcctg acggtgaaca gcatcacggc
     5581 cattctccgc gcgtcgtctc ccccggtatt gacgcttacg gcggaaggtg ttgtcctcac
     5641 gacaagctcg ccactcaatt tcggcaaggt tgaacttggg tctagcgccg cacggcggat
     5701 tgtcatcgcc aacaacacgt caggggcact gacgctttcc accgtcgcct tatcgggcga
     5761 agcctttgaa ctcgaaaccg tcttgacgct gccgcgagtc ttgcagcccc ggacggcggt
     5821 gaacctggaa accattttta cgccaaaagt ggtggggtcg ttcgaaggta aactcctggt
     5881 caacgaccgg ctgtttacgc tcaagggctc ggcggcgtcc ccgcctgtgc ctcgcgcgac
     5941 gatttcgatt tctcccgccg tgacctccag cgggaaacag ccgaaggtga gtgtcgaact
     6001 ggcctcaccc gccgcgttcg ccgtcacggg agtgctcaca ctcggctttc aggcgaacgc
     6061 ctcccacagc gaccctacca tccttttcgt gcgcggcggg tcgaagagca tcgggttcac
     6121 ggttgcacag ggcgaaacga gagcgacgtt tccgggggga gaaaccgaaa cggtgctgca
     6181 aaccggcaca actgccggga ccatcaccct ccagatcgtc tcgaacagcg ataccctctc
     6241 gattgcgatt ccggcatcgc ccgtcgtcgt cgatatggcg cgagggacac ggaccgcttc
     6301 cggtctcgac atcgaattga cggggttcga caatacacgt tcggtgtcgc agttgcggtt
     6361 ccaattcttc gaccgcaatg gacgtgccat cggctcagga ccggtttccg tggatgccgg
     6421 tgcggatttc cgcagcttct ttgacgcctc ggcgctgggc ggcgtctttt ctcttcgggc
     6481 ttcctttccg gtttccggcg atgcctcaca aatagcgggc gctgaagtgg aactggtgaa
     6541 ttcggc
//
LOCUS       DASTMK010000149        29009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_167670, whole genome shotgun sequence.
ACCESSION   DASTMK010000149 DASTMK010000000
VERSION     DASTMK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 29009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29009
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167670"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..822)
                     /locus_tag="VFQ79_06245"
     CDS             complement(31..822)
                     /locus_tag="VFQ79_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0139289.1"
                     /translation="MVPNSNHSHPLALITGASSGIGETFARRLAARGFNLVLVARRRD
                     RLEELRREFESSGDIAVEVLAVDLTQPAGMCVVEEKIASTENLQLVVNNAGFGSRGLF
                     HTISIEKQELMHRLHVIATMRLSHAALRRMAIQRSGSIVNVSSVAGFLQGPSNVSYCA
                     TKAWMNSFTEGLYLELRRARLPIKVQALCPGFTHSEFHDTMGMDRGVVPKNWWMTAED
                     VVDASLRGLERGDLFVVPGWRYNSFVKLLRLLPRPLRHTLVRNAR"
     gene            complement(843..1358)
                     /locus_tag="VFQ79_06250"
     CDS             complement(843..1358)
                     /locus_tag="VFQ79_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0139290.1"
                     /translation="MNYSEPPMEVYLLRHGIAENGKPGSRDADRALTPEGKDKLRETI
                     AVAGRAGLAPSLILTSPYRRAVETARIAAERLKYKNEPLVSDAFLPDSTPDQAWNEIR
                     LHKDADQLLIAGHEPLLGYLTAYLLGAPSIDVDFKKGAIVRVDLDRFGPHPRGTLKWM
                     LTPKLAARDNL"
     gene            1382..2614
                     /locus_tag="VFQ79_06255"
     CDS             1382..2614
                     /locus_tag="VFQ79_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU0139291.1"
                     /translation="MRSSGTALERAAFYFTCGSAVAILFSIAASHILLALALAALLMS
                     GVPLRFPPVKLPLALFFAGTVISMVLSSDPASGRPQIRKFFVFLLLLLVYSVFRHTGQ
                     ALWLIWLWIGVATLSSLRSLVQFSEKVAEAERLGRPFYTFYMTERITGFMSHWMTLGG
                     QLMIVLLMMSALVFFGRESTRRLVLWVICGAICSLGILLSFGRGVWLATALAGLYLVW
                     YWRKVLVLALPVVLAIVVVAGPDFIRARVRSSFQPHGDVDSNQHRVVTYRTGVEMIKA
                     HPLFGLGPEQVNAQFEKYLPGDIPRPLPTGWYGHLHNIYLHYAAERGIPTLLALLWLL
                     GKVLYDFWRALKKVPPGPSMEKAILHGAIAVVLAILIEGVFELNLGDTEVLSMFLIVV
                     ALGYVVRDNVLRPGSAHA"
     gene            2607..3329
                     /locus_tag="VFQ79_06260"
     CDS             2607..3329
                     /locus_tag="VFQ79_06260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0139292.1"
                     /translation="MPDGLKGRQIILAGGSGGLGSATARLLAHEPVRLIVSYRSNRDR
                     AILLSPLAAIIQADLASAEDRKHLLDEAPELYGLVVFSGDPARVSNPAELEEAMRRSH
                     EVNYLGPVLLAREAAERMKNSGTPGAIVLVATMQALALFPGSTAYAAQKAALVQAAKI
                     LAKECRGPANIRVNVVSPGVMQAGMAEASIRSGKYDSYISGGTISRFGRAEDIARAVR
                     LLLEPDSYITGQVLAVDGGMTL"
     gene            complement(3336..5978)
                     /locus_tag="VFQ79_06265"
     CDS             complement(3336..5978)
                     /locus_tag="VFQ79_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139293.1"
                     /translation="MNIRMLFGRGRRERELDEEINAHLAMAIRERIARGEEPQAAELA
                     AQREFGNRALIQETTRDMWGWSFLAQFLQDARYALRGMRNSPGFTAVAVLSLALGIGA
                     NTAIFSLMYTVMLRALPVTHPEQLVELLQKYPGEPRGNGYWTWRSYEHYRDHNHVFSA
                     LTGTAIDNRARLQTGDSEPATGVAEYVLGNYFPELGVKPALGRLIGPGHDPASPDGAV
                     AVVSWSLWASRFNRDPGVLGKQILVNDTPAVVIGVAPREFVGLRVNARTDVWLPFRPA
                     MKLALLGRLKPGVTLEQARAEMALLYRFTIEERAAASTDPQIRRMRVEVEPCGAGLSS
                     VRDRIGKPLTVLMAVVAVLLFLACVNIAGMLLARGASRAREMALRLGLGASRGRLVRQ
                     MLTESLMLSIAGTLAGAVVAYFGTTVLLRIIDSGREHERIHLEVQPDGGILLFAAGVA
                     ILTGLLFGLIPAVNAFRSTPALALRQAGRAVETRLSRLVGKTLLAAQVALSVLLLSTA
                     GLFIAHLSNLNQADLGFRRDHVLLVTLDPSRSGYRAERLSGAYQELLQRIQAIPGVRS
                     ASLSAPTPLHGAGASGFGTVEGFEERPEDRRWISISYVAPKYFETLETPLLSGREFDF
                     QDQVNPRVAIISRTLSRHYFAGRNPIGKRITLDHVTLSREPATYEIVGVAEDANYMEI
                     REPEERRIIYLPAFRNERVLAHTFVIRTGVGPWSITGDVRRAVRETAQTISVARITTL
                     SDQIDASIVLERMMAVLSGFFAVLGALLAGIGVYGLLAYTVARRTNEIGIRLALGATP
                     GGVLRMITWEAFIAVAAGLVLGLPMVIWGRTLAAALVQDLTTKTTASFAIGAAAIVAV
                     ALLASYIPARRAARVNPMESLRQE"
     gene            complement(5975..6340)
                     /locus_tag="VFQ79_06270"
     CDS             complement(5975..6340)
                     /locus_tag="VFQ79_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139294.1"
                     /translation="MGKTEAYKNRIELLQGTLDMLILQTLRWGSQHGYGIAQAIRSGS
                     GDVLQVETGSLYPALHRLEKQGWIRSEWKNSEYNQRAKYYQLTAAGKKHLASELSRWN
                     QLSAAIARLMNPAERESEA"
     gene            complement(6387..7892)
                     /locus_tag="VFQ79_06275"
     CDS             complement(6387..7892)
                     /locus_tag="VFQ79_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU0139295.1"
                     /translation="MNPSIGEPGKFPFTRGVYPGMYRSRLWTMRQYAGFGTAEESNRR
                     YRYLLSQGITGLSVAFDLPTQMGMDADHSLATGEVGRVGVAICSLRDMERLFEGIDLA
                     RITTSMTINSTAAILLAMYVLVAKSQGADPRKLSGTVQNDILKEYIARGTYIFPPRPA
                     MRIITDLFAWAGREIPEWNTISISGYHIREAGSTAVQEVAFTMANAIAYIQAALDAGL
                     DLDSFAPRLSFFFNAHSNFLEEIAKYRAARRLYARLMKERFGAKNPRSMMLRFHAQTA
                     GSTLTAQQPDVNVIRVTMQALAAVLGGAQSLHTNGRDEALSLPTEESVRLALRTQQII
                     GYESGVTATADPTGGSYYIEQLTDSIEAGAREYLDRIDALGGTLAAIEKGYIQGEIQN
                     AAYEYQKAIEKGESIVVGVNRFRMGEETPIPTFRLDPAIENQQIESLRQVRASRDAAR
                     VGTRLEALAAAARSGDNLMPHIIDAAEAHATVGEISDSLRQVFGIYRETGV"
     gene            complement(7889..8143)
                     /locus_tag="VFQ79_06280"
     CDS             complement(7889..8143)
                     /locus_tag="VFQ79_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139296.1"
                     /translation="MVRKFLQHVVPGVIRPLRVLWNQVIGFVFLAFAVFSSRSIYTAV
                     KEYDGDAESLFRVVLTGFFGAIMAYFGVFSFLRARRISRS"
     gene            complement(8177..8686)
                     /locus_tag="VFQ79_06285"
     CDS             complement(8177..8686)
                     /locus_tag="VFQ79_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139297.1"
                     /translation="MPVDELLEMILGVSGKDVERVFEILSRGTHVSGASRLRWAGFAP
                     IREDIESALARLPDAEPGRAFDPQRCSRAVLRGTAARIDLPRTAAEKRRLLRRHSFWD
                     QLLGVLASEEIAYVTYSYSEKADVYRAQLDPAGLARLRQSAGLLAYTKLEQQVRSAPI
                     TSIDLFVER"
     gene            8837..9763
                     /locus_tag="VFQ79_06290"
     CDS             8837..9763
                     /locus_tag="VFQ79_06290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0139298.1"
                     /translation="MDIENTNNPVERLVHLYEDGAFNRRELVRRIATYTGSIATAIAA
                     LGAFALPAEAQACPDDVRVPPDASDLESEPVTFPGQAGTVYGYLSRLRSLQVVPAQPA
                     VLVIHENRGLNDHIKDVTRRVARAGFVALGIDLLSRAGGTDWYPDPADALAAYNSLNS
                     QTHLEDMQSAVEFLRRMSFVDGSHLGAVGFCAGGGNCFNLAVTTPLQAAVVFYGAPPN
                     PVEQVGSLAGPVLMIYAELDRGFTSRVPAAVTALLQAEKTFGLHVYEGARHAFHNDTG
                     ALYNAEAACDAWAKTIAFFNRHLRPPADANEA"
     gene            9767..10633
                     /locus_tag="VFQ79_06295"
     CDS             9767..10633
                     /locus_tag="VFQ79_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139299.1"
                     /translation="MTPVAALAWLLLQTTQQNPAQAPKPAPQNPSPMVEHTRSHPRLQ
                     QETPPGERIPLGMGTLYVPAAAGRRSGVPLLVFFHGGKWLPEVGAHRTRQAAIVIQLG
                     AGSGTYAREFEDRERFPELLTEAEQKSGLRFGSITLGGWSAGCAAVREILRGEHAYER
                     ISNVLCIDGIHTGYVDGKPGPLESSIETANLATWLKVGRDTIAGRKRVVVTHSEIFPG
                     TFASTTETADYLVRELGIKRRAVLRWGPMGTQQLSEARQGKLLIVGYAGNSAPDHVDQ
                     LHSLPEYLRWLD"
     gene            10664..13819
                     /locus_tag="VFQ79_06300"
     CDS             10664..13819
                     /locus_tag="VFQ79_06300"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0139300.1"
                     /translation="MKIIFWAAAIALGLTGSAYAQRNTATIIGTVRDSSGGIISEASV
                     RAVNKATGVDYPTTSDRLGNFIVPDLPPGVYTVTVSQTGFKQYVQEELVLNVDQRPNL
                     DVVLQVGSVSDSITVDASAPMIEATHANIGGVVDKVNVQQLPLNGRQFLQLALLLPGT
                     SPAAGGQTVARGGGPRNVGFQGGGNRATNNTFLVDGVDSFGFRFKNTSLRPSVSSIQE
                     FKILESPYDAQYGVASGVHVSVITSSGTNQFHGELFEYIRNDKLDARNFFDQQKPPYR
                     QNQFGGAIGGPVIRNRTFFFGNYEGFRARRGQTNSAQVPSPEQLAGDFRSDPRAIIDP
                     LSGLPFPDKTIPQSRISEVSKRFFQFFPAPNSALAAPNFINSASSEVVEDQWVGKLDH
                     TFSEKWRVFGRYSYSKVESFVPGSVPLFGTINDMYVHNAVIGSTLILSPRTFLDVRIG
                     YNREDAINRSEQIGQIPSREFGIPGLFVPPEVDGVPQVAVQGFATIGDAINSPEGRVE
                     NSEQFIANWTRVQGRHTIRAGGNLWPIQLNKVFLSGIQRGQFNFQNIHAQGTTGLPEF
                     LLGLPQQAQRNIGLVRNDARTVLFNAYASDDVRLSRRRTLSFGLRYELRPSFIDKEDR
                     RAVFIPEGEGKFVVAGDPNNGFTGRMNRSLYRTPVKNFAPRFSLAADLTGDGKTVLRT
                     GYGIFYNMEIYNSQLLDALNPPFSIFQIYQADPGIGVILRWENPFDATDPRAGGLPSG
                     YGITPWFRQGYMQQWSFGLQRQLVENLALDVSYVANKGTALDGVRLINQGALPGASNA
                     VYVRQFPNFGYHITADSHGDSIYHSLQSRITRRFSKGLTFMASYTWGHAIDNSNGEGN
                     GSGGQFFAMDDRNLRLDRGSSDFDTRHRATFSGIYSLPFGRGRLLSGWQIAAMYQVQT
                     GFPSTVGQSGNRSGTFAIGERADILCDGRLGRGERTRTRWFDTACFAPSPLGKFGNSS
                     RGAIVRPGQNNLDFSIIKDTKVTERMQVEFRAEFFNLPNHTQFSMLGNLGTSVTTPAT
                     FGVITAAQDPRIIQFGLKLKY"
     gene            complement(13827..14909)
                     /locus_tag="VFQ79_06305"
     CDS             complement(13827..14909)
                     /locus_tag="VFQ79_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0139301.1"
                     /translation="MNRRLFLQTAAAAALSRPAAANTGFLKLGYDTYSLRAFRWKALQ
                     HLDFAAAQKLDTIQLSGLGDYESLDPAYLQKVKDAATRHGISIDAGIGCICPTTKSWN
                     PKTGDPVEYIRTGLRVAKAVGATSMRCFIGSSADRLGSLPIEAHIEKTVEVLRAARSH
                     AADAGVKIAIENHSDLQARELKALIEEAGKDYVAACLDTGNPMSVVEDPVVTMEVLGP
                     YTVTTHVRDSVVYEHPRGAAMQWTAVGDGILDFKRILELHRRLCPAASMQLEIITGRP
                     PRVLPYLESEFWAAFPKTDAAEFARFVALAKRGRPFSGMMIIADAGTKVPPEYQAALK
                     QQQMVDLEHSLEYCRKTLDAGVRWRT"
     gene            14947..15225
                     /locus_tag="VFQ79_06310"
     CDS             14947..15225
                     /locus_tag="VFQ79_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139302.1"
                     /translation="MTPLEKVEHEIRRYEHTLLKFSAREKKDGSVELVIELKEPAPGV
                     HTYYAPIHPRDIENSQFPWTFQRYLYDCLHDYLVELFVRTPQSREAQP"
     gene            15222..16190
                     /locus_tag="VFQ79_06315"
     CDS             15222..16190
                     /locus_tag="VFQ79_06315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0139303.1"
                     /translation="MTEAGRILAEEIQREGPIPFRRFMEVALYHPKFGYYTSGKDPFG
                     RAGDFYTAEQIQPVFGILMARYIRSLLDEMGGGTGVVLELGAGRQEMKPFLSDLEYVP
                     VEAGKGTMPARFSGVVFANEFFDALPVDVAVLREGKYREMLVDFDGNGFVWRDGNAVR
                     PEVREYLDRFAAAEEEGCIREVHLESLAWVEKISAALENGFLIVIDYGYTRRELMRFP
                     RGTLMSYRRHTASEDVLKDPGERDITSHVFFTTLEDVAASHGFRTAAFESLSRTLLRV
                     GEADWFASVLEGVSESEQLRRRLQLKTLLFGMGETFRTLVLGRGPK"
     gene            16553..17203
                     /locus_tag="VFQ79_06320"
     CDS             16553..17203
                     /locus_tag="VFQ79_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU0139304.1"
                     /translation="MRSRAFLMGVAVVLLLSGCGKKKPQAAIPRPAGWQEKGIASWYG
                     NPYHGRAAANGEIYDMEKLTAAHRTLPFDTWVRVRNLSNGKTVEVRITDRGPFVGDRI
                     IDLSRAAARNIDMIGPGIVNVRLQVIAPPRYVQPVDYFAVQVGAFQERKRAERLRQEM
                     EKRYGPARVVIRDGKPPLWRVLVGRAPTLEAANGIAEQLRAARHSAFVVRLDDPAM"
     gene            17251..18297
                     /locus_tag="VFQ79_06325"
     CDS             17251..18297
                     /locus_tag="VFQ79_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0139305.1"
                     /translation="MSYMGISGCPPICADRDLVGDPIGVRNTLAEIERAGRNRLPVLL
                     LGETGTGKGIIARRIHAVRGTGPFVTIDCSALVGELMESELFGHVKGSFTGAVSGKIG
                     LIEMADGGTAFLDEIGELPLELQAKLLRVIQEKEFRPVGSTQIRRSDFRVIAATLRDL
                     ATDVAEKKFRSDLYFRLKGLTIRLKPLRERREDIPVLVNHFLRRYGNNHSITAEVMDA
                     LVAYDWPGNVREVELCVQHMIALSPEPLIHTAALTSMVLNDMRQRRTAYRVHGAAAGV
                     ETLQREFLPASPVPDSIGPAVFGSEVIPIPELERRAILHALEHTKGDRTMAAHLLQIG
                     RTTLYRKIRDYGLD"
     gene            complement(18289..19086)
                     /locus_tag="VFQ79_06330"
     CDS             complement(18289..19086)
                     /locus_tag="VFQ79_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU0139306.1"
                     /translation="MKNLIPGALVCLALGSGLFAVESSQFHWDGEIGKGQVLEIKGVN
                     GGIAAEAASGSRAEVSARKSANRSDPASVEIVVVPHSGGVTICAVYPSKDAQPNECKP
                     GNSGRNNVSNNDVQVHFTVRVPAGVKFVGKTVNGKIDARQLQGDAEAYTVNGQVAIST
                     NGNAQARTVNGAIDVVCGDANWTEAREFKTVNGAISVTLPETTNTAVNANTLNGGIET
                     DFPLTVSGFVGKKLTGTIGSGGRQLNLSTVNGRIRILKQGGRRTGSV"
     gene            19189..19674
                     /locus_tag="VFQ79_06335"
     CDS             19189..19674
                     /locus_tag="VFQ79_06335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0139307.1"
                     /translation="MAKQYPSAPAMAIEPNKSYTAVFETSKGNIECQLFAEDAPVTVN
                     NFVFLAREKFYDGTVFHRVIKDFMIQGGDPTATGTGGPGYRFADEVKNNPRKHQVGSL
                     SMANAGPNTNGSQFFITHVVTQWLDGKHTVFGQVTSGQDVVNAIQQGDQLKSVTIQES
                     A"
     gene            complement(19690..21630)
                     /gene="speA"
                     /locus_tag="VFQ79_06340"
     CDS             complement(19690..21630)
                     /gene="speA"
                     /locus_tag="VFQ79_06340"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEU0139308.1"
                     /translation="MKLSEGATTWEPSERWTVTDAAELYDVASWGKGYFSVGENGHLW
                     VHPDKDVNRRTDLKELVDKLVLRGISVPILIRFADILKHRLGDLYQAFQSSITEHGYK
                     GDYVCVYPIKVNQQRHVVEEVFRFGRPYGFGLEAGSKPELLAVLAVADNETPVICNGF
                     KDDEYIETVMLARKMGRQITPVIEKFTELDLIVKHAQNVGVRPAIGLRVKLASRGAGK
                     WKSSGGYRSKFGLTVSEALRVLQRLKELGMEDCLELLHFHLGSQITNIRHIKGAVTEA
                     ARVYVELKRAGAGLKYMDVGGGLGIDYDGSQTDFESSVNYTLQEYANDVVYHIQNICD
                     EAEVPHPTIVTESGRAVAAYHSVLVFNVLGVTGLGEEDVPQDLPEEAEQPLVDLHQTY
                     RSLSMKNLLEGFHDAQQALDAALNLFSLGYLPLEQRSLAENLYWGIGRKIQKMIKDME
                     YVPEELEGLEAMLSDTYFCNFSLFQSMPDSWAVKQLFPIMPIHRLGEPPTRNAILGDI
                     SCDSDGKIDQFIDRRDVKKTLPLHTFNGKHYYLGAFLVGAYQEILGDLHNLFGDTNAV
                     HVSLDASGEVVLDAVIQGDTVREVLNYVQFSAQTLLEEFRRDAETALREGRIGYQELA
                     RLLRFYEEGLRGYTYLEDGRQQ"
     gene            complement(21802..24456)
                     /locus_tag="VFQ79_06345"
     CDS             complement(21802..24456)
                     /locus_tag="VFQ79_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0139309.1"
                     /translation="MAPGAAIAHYRVLSRLGAGGMGEVYLAEDTRLERLVALKILPER
                     IAADSERMRRFVQEAKAVAVLSHPNIAHIYDIDEADGVHYMAMEYVEGQTLAARLNGR
                     PLPTSDIIEIGTQIAEALAEAHSKGITHRDIKPANVMITPRGQAKVLDFGLAKVRKPD
                     HTLQTETVSGRLTDAGIVMGTVQYMSPEQALGQDVDHRTDIFSLGSVLYEMATGRRAF
                     PGPTTAAVFSALLNKHPDPPSKVNGSIPPELDRIVLKALEKDAELRYQTASDLTADLK
                     RTKRDADSGRTTAPAEVGSRSLRGRSRLPLLIVSVAAMIALASWYFNSRSSARTEPIA
                     LKATPLTAYPGNESQPSFSPDGSQVAFTWNSETEENWDIYVKVVEAGAPLRLTSSPAT
                     DFSPAWSPDGRQIIFLRQESDSAAFYLIPPLGGLERKLADANPQRTGVDAPYASWSPD
                     SNTVALVDKTAQQEPMSIFLHNIETGERRKLTNPPDKTYGDSSPQFSADGRMLAFVRT
                     GALAVQDIYVVPVAGGEPERLTFDNRRIYGLAWNPSDGLIYFSSARAGSSRLWKISPK
                     GGAPERIIGIGENASFIAISPQGKRLAYTRSILDTNVWRFDLTGSAGAAGKGTRLISS
                     TRYEQGPQYSPDGRRIVFASNRSGTPEIWLCDKDGNFPTRLTYFEGAATGSPHWSPDG
                     KTIAFDSRPNANPDVYIVSAEGGTPRRMTTEPSDDVVPTYSNDGRWIYFASNRTGTYQ
                     LWKIPAEGGAGIQVTKNGGFYGIESPDGKYIYYAKSPRAPGLWRVPIAGGDEEPVLES
                     LRAGFWGYWAIVPRGVYFLERREIQDIGIRYYLRLLDLKTMRESTVMPIEKRPFNAGL
                     SVSPDGRHFLYTQVDQSDTDIMLVEDFR"
     gene            complement(24483..24995)
                     /gene="folK"
                     /locus_tag="VFQ79_06350"
     CDS             complement(24483..24995)
                     /gene="folK"
                     /locus_tag="VFQ79_06350"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU0139310.1"
                     /translation="MTTVYLSLGSNLGDRERHLQRAVECLDSAGLQVLRISSVFETDP
                     VDKLDQPCFLNTVIEARTSLLPRQLLARTSKLERRLGRKRLVDKGPRTIDIDILLYGA
                     AIVNTHDLTLPHPRMTERRFVLEPLAELAPGLRHPVTRRTIAELLASTMSQGCRKTGI
                     ELHLHQAGMP"
     gene            complement(25000..26025)
                     /gene="ruvB"
                     /locus_tag="VFQ79_06355"
     CDS             complement(25000..26025)
                     /gene="ruvB"
                     /locus_tag="VFQ79_06355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU0139311.1"
                     /translation="MREKGIVSPAPRDEERQFEAGLRPRRLDDFTGQAKLKENLAIAI
                     EAARRRDEAMDHVLLYGPPGLGKTTLATIIASELEAGFEQTSGPILQKKLDLTGILSN
                     IRARQVFFIDEIHRLLPDVEEMLYSALEDFRVDILIGAGPGARTHSLPMPRFTAIGAT
                     TRQGLVSAPLRGRFGLVLRLNHYDVAELERIVLRSAGLLDVAIDEEGAREIARRSRGT
                     PRIANRLLRRVRDYAQVRAEGHIDHGIATTALDLLEVDRFGLDEIDQKIMLTVIEKYS
                     GGPVGLNTIAASIDEEPATIEEVYEPYLMQLGFLDRTPRGRVATDHAFDHFNIPRHMR
                     GLQRSLF"
     gene            complement(26051..26650)
                     /gene="ruvA"
                     /locus_tag="VFQ79_06360"
     CDS             complement(26051..26650)
                     /gene="ruvA"
                     /locus_tag="VFQ79_06360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU0139312.1"
                     /translation="MIAYLRGTLLEKHPNQVVVDVAGVGYDVTIPVSTFSSLPDTNSE
                     VRLRIHTHVREDALLLFGFLTMDEKSLFEKLIGVSGIGPRLAITILSGLPASDLITAI
                     RGAQLEHLVRIPGIGKKTAERMVLELRDKVAGIGAGAAGIPAQKQPALEPVEEDILSA
                     LLNLGCNRGAAETALRKAKAAGAPAEFEPLFRRALELVR"
     gene            complement(26737..27117)
                     /locus_tag="VFQ79_06365"
     CDS             complement(26737..27117)
                     /locus_tag="VFQ79_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0139313.1"
                     /translation="MRAVDTNVLVRLITRDDLRQAASAEAFIENGAWVSVLALAEAIW
                     VLSAVYELGPKDQAKAIEMLLNHRDLVLQEPETVAAALALFRVKPALGFSDCLMFHLA
                     RKAGHLPMGTFDRNLAKVEGAQKL"
     gene            complement(27104..27367)
                     /locus_tag="VFQ79_06370"
     CDS             complement(27104..27367)
                     /locus_tag="VFQ79_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU0139314.1"
                     /translation="MAIARSKITAQGQISIPADVRRKLGAGPGSIVEWDDEDGKIVVR
                     RAGRFTSEDIHRALFGARTPKKRGLAELKSGIRNYVWKRHARG"
     gene            27448..28185
                     /locus_tag="VFQ79_06375"
     CDS             27448..28185
                     /locus_tag="VFQ79_06375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEU0139315.1"
                     /translation="MPNLIVGMARRLFFVNEVHSDRAEISGEDAKHLTRVLRVERGQR
                     YEISDNRTLYLAEIETARKEHVVFRIIERLEYTAPTLTISLCPALIRFERFEWILEKA
                     TELGVAAITPFAAVRSEKGLDRASAKRMARWRKIVLESSQQSRRPVMPVLHEAIPFQV
                     VLRAETQRKLLLDEAREAPPIRSVLTASESIAVLTGPEGGWTDAERAGAIAAGWTPVS
                     LGPNILRAETAAVAALAVLFCASDSWR"
     gene            28202..>29009
                     /locus_tag="VFQ79_06380"
     CDS             28202..>29009
                     /locus_tag="VFQ79_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139316.1"
                     /translation="MNFSRRQFLGAAGAAALSTARSYSQVAGAGERLRIGVIGCGGMA
                     NGHMRTLMKMRESDNVEITAVCDIYDKRLDAAAQLTGAKPVKDYRTLLQAKDIDYVLI
                     ATPEHWHYQMTMDAADAGKHIYCEKPMTHTVEESRKVMDKITKTGLKMQVGVQGMSDE
                     SYATAYEYMTKGALGKVVLAQIDYSRNYKGDFWAYPIDEGVRPGENLNWNAWLGSAPK
                     HPWDPERYFRWRRYWDYSGGIASDLFVHRVTRIIKALGLKFPSMGVATGGK"
BASE COUNT         5671 a         8500 c         9038 g         5800 t
ORIGIN      
        1 aaccgccgcg cacgttaaag aagtaagcgc tcagcgggcg tttcgaacca gcgtatgccg
       61 cagaggccgc ggcagcagtc tcaggagctt gacgaaggaa ttgtaccgcc agcccggaac
      121 cacgaacaga tccccgcgct ccaggccacg cagtgatgcg tcgaccacat cttccgctgt
      181 catccaccag tttttgggga caacgccacg atccatcccc atggtgtcgt gaaattcgga
      241 atgcgtgaaa ccggggcaca acgcctgtac ttttatgggc aggcgggccc tgcggagttc
      301 cagatacagg ccttccgtga aactgttcat ccaggccttc gtggcacaat aactcacgtt
      361 gctcggcccc tgtagaaaac ccgcgacgga ggaaacgtta acgatactgc cacttcgctg
      421 gatggccatt cgccggagag cggcgtgact aagccgcatg gtggcgataa catgcagccg
      481 gtgcatgagc tcctgctttt cgatcgaaat cgtgtggaac aatcctcgcg agccgaagcc
      541 ggcgttgttg acgacgagtt gcagattttc ggtggaagcg atcttctctt caacgacgca
      601 catccccgca ggctgggtga ggtcgacggc aagcacctcg acggcgatat cgccgctgga
      661 ttcgaactcg cgccgcaact cctcgaggcg gtcgcggcgt cgtgccacaa ggaccaggtt
      721 gaatccgcgg gccgcgagac gccgggcgaa tgtttcgccg atgccgctgg aggctccggt
      781 aatcagcgcg agtggatggg aatggtttga attagggacc ataaaaaaat cgggaccgaa
      841 cctcacaaat tgtcacgggc agccagtttc ggcgtaagca tccatttcag ggttccccgg
      901 ggatgcggtc cgaaccggtc gagatcgacc cggacgatag cgcctttctt gaaatcaacg
      961 tcgatggatg gtgcgccgag cagatacgcg gtcagatacc cgagcagggg ctcgtgaccg
     1021 gcgatcagaa gttgatccgc atctttgtgc aggcggatct cattccatgc ctgatccggg
     1081 gtgctgtccg gaaggaaggc gtccgagaca agcggctcgt ttttatactt gagccgttcg
     1141 gcggcaatcc gcgccgtctc caccgcgcgc cggtacggac tggtaagaat gagcgaaggc
     1201 gcaaggccgg cgcgcccggc aaccgcgatg gtttcacgga gcttgtcctt gccttcggga
     1261 gtaagagcgc gatcggcgtc gcgtgagccg ggcttgccgt tttcggcgat gccatgcctc
     1321 aacagataga cttccattgg tggttctgaa tagttcattc tgtcatactg ggattcgtcg
     1381 aatgcgctca tccgggaccg cgttagagcg agctgccttc tatttcacct gcggctccgc
     1441 cgtagcaatc ctgttttcca tcgcggccag ccacatcctt ctggcgctgg cgctggcggc
     1501 gctgctgatg tccggggtgc cactgcggtt tccgcctgtc aaactaccgc ttgccttgtt
     1561 ttttgcgggc acggttatct cgatggtgct ctcgtccgat ccggcatcgg ggcgtccgca
     1621 gatccgcaag ttttttgtct ttcttttgct gctgctagtg tacagcgtgt tccgccatac
     1681 cggccaggcg ctgtggctga tctggctgtg gattggggtc gccacgttat cctccttgcg
     1741 aagcctggtg caattttcag agaaggttgc cgaagcggag cggctcggcc ggccgtttta
     1801 taccttctac atgaccgagc ggattacagg attcatgagt cattggatga ccttgggcgg
     1861 gcagttgatg attgtgctgc tgatgatgtc ggcgctggtg ttcttcgggc gggaaagcac
     1921 gcggcgcctg gtgttatggg tgatttgtgg cgcgatctgc tcgctcggca tactgctgag
     1981 ttttggacgg ggcgtatggc tggcgacggc tcttgcagga ctgtatcttg tctggtactg
     2041 gcgcaaggta ctggttctgg ctctgccggt ggtgctcgcg attgtggtgg tggccgggcc
     2101 ggatttcata cgcgcgcgag tgcgatcgtc gttccagccg cacggggatg tcgactccaa
     2161 tcagcaccgg gtggtgactt accgcacggg agtggagatg atcaaagccc atccgctatt
     2221 cggcttgggg cctgagcagg tgaatgcgca gttcgagaag tacctgccgg gcgacatccc
     2281 gcggcccttg cccacgggct ggtacggcca tctgcacaac atctatttgc actatgcggc
     2341 ggagcgtgga atccctaccc tgctggcgct gctgtggctt cttgggaagg tgctctacga
     2401 tttctggcgc gctttgaaaa aagtaccgcc gggtcccagc atggagaaag cgattcttca
     2461 tggcgcgatc gcggtggtgc tcgccattct catcgaaggt gttttcgagc tcaacctggg
     2521 cgatacggag gtgttgtcca tgttccttat cgtcgtagct ttgggatacg tggtacggga
     2581 taacgtactc cggcccggta gcgcccatgc ctgacggcct caagggccgg cagatcatcc
     2641 tggcaggcgg ttccggcggg ttgggaagcg ccactgcccg gttgctggcg cacgagcccg
     2701 tccgtttgat tgtgagttat cgatcgaacc gggaccgggc cattcttctt tctccgttgg
     2761 cggcgatcat ccaggcggac cttgcctcgg cggaagaccg gaaacacctg ctcgatgaag
     2821 caccggagtt gtatgggctc gttgtgttct ccggcgaccc ggcgcgcgta tcgaacccgg
     2881 cggagttgga agaggcgatg cggcggtctc acgaagtgaa ctatctcggt cccgtgctgc
     2941 tggcacgcga ggcggccgag cggatgaaga actcgggtac gcccggcgca atcgtgctgg
     3001 ttgccaccat gcaggcgctc gcgctgtttc ccggttcgac cgcctatgct gcccagaaag
     3061 ccgccctggt tcaggcggca aaaattctgg ccaaggaatg ccgcggaccg gcgaacattc
     3121 gcgtcaacgt ggtgtcgccg ggcgtgatgc aggcgggcat ggccgaagcc agcatccggt
     3181 cgggaaaata cgacagctac atttccggag gcacgatttc gcgcttcggc cgggcggaag
     3241 atatcgcgcg cgcggtgcgc ctgttgctgg agccggacag ctatattacc gggcaggtgc
     3301 tggccgtgga cgggggcatg acgctgtaag tcgttctatt cctgccggag agattccatc
     3361 ggattcacgc gagcggcccg ccgggccgga atatacgaag ccagaagcgc cactgcgacg
     3421 attgctgccg ctccaatcgc gaatgacgca gtggtctttg tggtgaggtc ctgaaccagc
     3481 gccgcagcca gtgtccggcc ccagatgacc atcggaagcc cgagaacgag acccgcggca
     3541 acggctatga acgcttccca cgtgatcatc cggagcacgc cgccgggcgt cgcgccgaga
     3601 gcgaggcgaa tgccgatttc attggtgcgg cgcgccaccg tgtacgccag cagaccgtag
     3661 acgccgatgc ccgccagcag cgctcccaaa acggcgaaga accccgacaa gacggccatc
     3721 atccgctcca ggacgatgga ggcgtcgatc tggtccgaga gcgttgtgat gcgggctacg
     3781 gaaatggtct gagccgtctc gcgtaccgct cgcctcacgt cgccggttat gctccagggt
     3841 ccgacgccgg ttcgaatcac gaaggtatgg gccagcactc tttcattgcg aaaggcgggc
     3901 aggtagatga tgcgccgctc ctccggctcg cgaatctcca tatagtttgc gtcttcagcc
     3961 acgcctacga tttcataggt agcgggttcg cggctgagcg taacgtggtc cagtgtgatc
     4021 cgtttcccga ttggattgcg gccggcgaag tagtgtcgcg acaatgtccg gctgatgatc
     4081 gccacgcgtg gattcacctg gtcctgaaag tcgaactcgc gtccggagag gagcggtgtt
     4141 tcgagcgttt caaagtactt cggcgcaaca taactgatcg agatccagcg gcggtcctcg
     4201 ggcctttcct caaaaccctc gacggtgccg aaacccgacg ccccggcgcc atgcagtgga
     4261 gtcggcgcgg agagagaagc cgatcgtacg ccgggaattg cctgtatgcg ttggagcaac
     4321 tcctggtacg cgccggacaa tcgttcggcg cggtatccgc ttcgagatgg gtcgagcgtc
     4381 accaataaca cgtgatcgcg gcggaagccg agatcggcct ggttgagatt ggacagatgg
     4441 gcgatgaaca gaccggcggt gctcaacagc aacaccgaca acgctacctg cgctgccagg
     4501 agcgtctttc caaccagccg gctcagccgc gtttcgaccg ctcttccagc ttgccgtaac
     4561 gccaatgcgg gcgtggatcg gaacgcgttc accgcaggga tcaacccgaa gagaagcccg
     4621 gtgagaatgg ctactcccgc ggcgaacagc aggataccgc catcgggctg cacctcgaga
     4681 tgaatgcgct cgtgttcacg tcccgaatcg atgatccgca gcaataccgt tgttccgaag
     4741 taggcgacaa ccgctcccgc cagggtgccg gcaatcgata gcatcaagga ctcggtcaac
     4801 atctggcgca ccagccggcc gcggctcgcg cccagtccga gccgcagtgc catttcgcga
     4861 gcgcggctgg ctccgcgcgc cagaagcatc ccggcgatgt tcacgcaggc gagaaacaac
     4921 agcaccgcca ccacggccat tagtacggtc aggggcttgc caatgcggtc gcggacggac
     4981 gaaagtcccg cgccgcatgg ctccacttcg acacgcatcc ggcgaatctg gggatctgtg
     5041 ctggccgcgg ctctttcttc gatggtgaac cggtaaagca acgccatttc agcgcgcgcc
     5101 tgctccagtg tcacgccagg tttcagccgg cccaacaggg ccagcttcat ggccggcctg
     5161 aagggcagcc agacatcggt cctggcattg acccgaagtc ccacgaactc gcgcggcgct
     5221 accccgatga cgaccgccgg tgtgtcgttc acaagaatct gcttgcccag gacgcccgga
     5281 tccctattga acctgctggc ccagagagac cagctcacga ctgcaacagc gccgtcaggg
     5341 ctcgccgggt cgtgcccggg accgatcaga cggccgagag cgggcttcac tcccagttcc
     5401 gggaaatagt ttcccagcac atactcggcg actccggtcg cgggttccga atccccggtt
     5461 tgcaggcgcg cgcgattgtc gatggcggtg ccggtaaggg ccgaaaagac gtgattgtga
     5521 tcgcgatagt gctcgtagct ccgccatgtc caatagccgt ttcctctcgg ctcgccggga
     5581 tatttttgga gaagctccac aagctgctcg ggatgcgtga caggcaatgc gcggagcatg
     5641 actgtgtaca tcaggctgaa gatcgccgtg ttggcgccga tgccgagagc cagcgacaac
     5701 accgcaactg cggtgaatcc cgggctgttc cgcattccgc gaagagcgta gcgggcatcc
     5761 tggaggaact gcgcgagaaa actccatccc cacatgtcgc gcgtggtttc ctggatgaga
     5821 gctcgattgc cgaattcgcg ctgcgccgcc agttcggccg cttgcggctc ctctccgcgt
     5881 gcaatccgct cacgaatcgc catcgcgaga tgtgcattaa tttcctcgtc cagctcccgc
     5941 tcccggcgtc cgcgtccgaa caacatccgg atgttcatgc ttcgctttcg cgttctgccg
     6001 ggttcatcag cctggcaatg gctgcgctca actggttcca gcggctcaat tcactggcga
     6061 gatgcttctt tccggccgct gtgagctgat agtatttggc ccgctggttg tactcagaat
     6121 tcttccactc cgagcgaatc caaccctgtt tttcaagacg atgcaacgcc gggtagagcg
     6181 acccggtttc cacctgcagc acgtcgccgg agccggagcg gatagcctga gcgatcccgt
     6241 atccgtgttg tgatccccag cgcagcgtct gaaggatgag catatcgagc gttccctgta
     6301 gaagctcgat ccgattctta taggcttcgg tctttcccat ggatgtaggt attctacatc
     6361 catccacgta gattgtctac agggtatcag acgccggttt cgcgatagat tccgaacacc
     6421 tggcgcagcg agtccgagat ttctcccacg gtagcgtggg cctcggcggc atcgatgatg
     6481 tgaggcatca ggttatcccc actccgcgcg gcggcggcaa gggcttccag gcgggtacct
     6541 actcttgcgg cgtcgcggga agcgcgcacc tgccggaggc tctcgatctg ctggttctcg
     6601 atagcggggt ccagccggaa ggtgggaatc ggcgtttcct ctcccatgcg gaagcgattc
     6661 actcccacga cgatggattc gcccttctcg atggctttct ggtattcgta cgccgcgttc
     6721 tgaatctcgc cctggatgta ccccttctca atagcggcca gcgttccgcc gagcgcgtcg
     6781 attcgatcca ggtactcgcg cgcgcccgct tcgatggaat cggtcaactg ctcgatgtaa
     6841 tagctgcctc cggtgggatc ggccgtagcc gtgacaccgc tttcataacc gatgatctgc
     6901 tgcgtccgga gcgccagccg gacggattcc tcggtgggca gcgacagcgc ttcgtcgcgg
     6961 ccgttggtat gcagcgactg tgcgcctcct aaaacggcgg ccagcgcctg catcgtgacg
     7021 cgaatcacgt tcacgtccgg ttgttgtgcc gtcagggtcg atccggcggt ttgcgcatgg
     7081 aaccgcagca tcatggagcg tgggttcttc gcgccgaagc gttctttcat caggcgggcg
     7141 tagagccgcc gcgcagcccg gtacttcgcg atctcttcca ggaagttgct atgagcgttg
     7201 aagaaaaacg agagccgggg ggcgaaggaa tccaggtcga gaccggcatc gagggccgcc
     7261 tggatgtagg cgatcgcatt cgccatggtg aacgccactt cctgcacagc cgtggaaccg
     7321 gcttcgcgga tgtgataacc gctgatggag atcgtgttcc actcgggaat ctcccggccc
     7381 gcccaggcga acaggtccgt gatgattcgc atggccggcc gcggcggaaa aatgtacgtg
     7441 ccgcgggcga tgtattcctt cagaatgtcg ttctgaaccg ttcccgataa ctttcgcggg
     7501 tccgcaccct gcgacttcgc caccagcacg tacatcgcaa gcaaaatggc cgcggtggag
     7561 ttgatggtca tcgaggtagt gatgcgcgcg aggtcaatgc cctcaaacag tcgctccatg
     7621 tcgcgaagcg agcagatggc gacaccgacg cgcccgacct cgcctgttgc gagcgagtgg
     7681 tccgcgtcca tgcccatctg cgtcggtaaa tcgaacgcga ctgacagtcc ggtgatgcct
     7741 tgggaaagaa gataccggta gcggcggttg ctttcttccg ccgtgccgaa gccggcatac
     7801 tggcgcatgg tccagaggcg gcttcgatac atgccggggt aaacgccgcg ggtaaacgga
     7861 aatttgccgg gctccccgat ggaagggttc atgaacggga gattctgcgg gcgcgcagga
     7921 aagagaaaac cccgaaatag gccatgatcg cgccgaagaa tcccgtcagc acgacgcgga
     7981 acaggctttc ggcatcgcca tcgtattcct tgacggcggt atagatcgaa cggctgctaa
     8041 aaaccgcgaa cgccagaaat acaaatccaa tgacttgatt ccacagcacg cgcagagggc
     8101 gaatcacgcc cgggacgaca tgttggagaa acttgcgaac catgctggat gcttctcctt
     8161 aaatcatatc ttcttttcac cgttcgacga acagatcgat cgacgtaatc ggagcggagc
     8221 ggacctgttg ttcgagcttg gtatatgcga gcagcccggc gctttggcgc aggcgggcca
     8281 gtcccgccgg atcgagctgg gcgcgataaa cgtcggcctt ttcgctatag gaataggtaa
     8341 cgtaagcgat ctcttcggaa gccagcacgc cgagcaactg gtcccaaaaa gaatgacggc
     8401 gcagcagacg gcgcttttcc gctgccgtgc gcggaaggtc gatgcgggcg gcggtcccgc
     8461 ggagcacggc acgcgagcag cgctgcggat cgaatgcacg ccccggttcc gcatccggca
     8521 ggcgcgccag ggcggattca atgtcttcgc ggatgggcgc gaatccggcc caccgaaggc
     8581 gggaagcgcc gctgacatgt gtcccacggg aaaggatttc gaagacccgt tccacgtcct
     8641 tgcccgagac cccgagaatc atctccagca attcgtcgac tggcatatcg cgggcgacaa
     8701 ccggggttat ggaaatcgat cccgcggctt cggagctgat tttgacgcga attgtatccg
     8761 gcagcggcac tctctgataa atttaacgcg tccggcggcc ctgttgagca tagaatcgac
     8821 ggaaaggagt ttgccgatgg atatcgaaaa caccaacaac cccgtagagc gcctggtgca
     8881 cctttacgag gatggggctt tcaaccgccg cgaactggtt cgcaggatcg ctacctatac
     8941 gggaagcatc gcaaccgcta tagccgcgct cggtgcgttc gcgctaccgg ccgaggcgca
     9001 ggcttgtccg gacgacgttc gggtgccgcc ggatgcgtcg gacctcgaat ccgaaccggt
     9061 gactttcccg ggacaggccg gaaccgtgta cggatacctg tcacgcctcc ggagcctcca
     9121 ggtggttcct gctcaaccgg cggtgcttgt gattcacgag aaccgcggtt tgaacgatca
     9181 catcaaagac gtaacgcggc gtgtggctcg cgccggattc gttgcgctgg gcatcgatct
     9241 gctttcaagg gcgggcggga cagactggta tccggacccg gcggatgcac tggcggcgta
     9301 taacagcctg aacagccaga cccacctcga agacatgcaa tccgcggtgg agtttctgag
     9361 gcggatgtcg tttgtggacg gtagccattt gggcgcggtg ggcttctgcg ccggaggagg
     9421 caactgcttc aacctggccg tgaccacacc cttgcaagcg gccgtggttt tctatggcgc
     9481 gccgccgaat ccggtggagc aggttgggag cctggccggg cctgttctga tgatctatgc
     9541 cgagctggac cgcggtttca caagccgggt acctgccgcg gttaccgctc ttctccaggc
     9601 ggagaagact ttcggcctgc acgtgtacga gggagcccgc cacgcctttc acaacgacac
     9661 gggcgctctt tacaacgcgg aagccgcttg tgatgcgtgg gcgaagacga tcgcgttctt
     9721 taaccggcat ctgcgtccgc cggcggacgc aaacgaggca tgacgaatga ctcccgtcgc
     9781 ggcgctggcc tggttgctgc tgcaaaccac acagcagaac cccgcgcaag ctcccaagcc
     9841 agcgccgcag aatccttctc cgatggtgga gcacacacgc tcgcatccgc ggctccagca
     9901 ggaaacaccg cccggcgagc ggataccgct cggaatggga acgctctatg tgccggctgc
     9961 ggcaggccgg cggtccggcg tgcccctcct ggtttttttt cacggtggca agtggcttcc
    10021 ggaggtcggg gcgcatcgga ctcgccaggc agctatcgtt attcagcttg gcgccggctc
    10081 tggaacctac gcgcgggaat tcgaagaccg ggagcgcttt ccggaactgt tgacggaagc
    10141 ggaacagaaa tcgggtctgc gcttcggttc gatcacgctg ggcggctgga gcgcgggctg
    10201 cgccgccgtt cgcgagatcc tgcgtggaga gcatgcttat gaacgtattt cgaatgtgct
    10261 ctgtatcgac ggcattcata cgggatacgt ggatggaaag ccgggaccgc ttgaatccag
    10321 catcgaaacc gccaacctcg cgacatggct gaaggtggga agagacacta tcgccgggcg
    10381 caagcgggta gttgtaacac actccgagat ttttccaggc acgttcgcca gcacgacgga
    10441 gacggcggac taccttgtgc gtgagttggg aatcaaacgg cgggcggtgc tgcgctgggg
    10501 ccctatggga acgcagcaac tgagcgaagc ccgccagggc aaattgctga ttgtggggta
    10561 cgcaggtaac tcggcgcccg accacgtaga tcagttgcac tcgttgcccg agtacctgcg
    10621 atggctcgat tgaatgacgt taagacgata gaggggaatc gccatgaaga ttattttctg
    10681 ggcagccgct atcgcgctcg ggctgacagg ctccgcctac gcccagcgca acaccgccac
    10741 cattatcggg accgttcgcg atagcagcgg cggaatcatc tccgaagctt ccgtgagggc
    10801 ggtgaataag gcaacggggg tggattatcc caccaccagc gaccggttgg gtaacttcat
    10861 cgtgccggac cttccgccgg gcgtctacac ggtgaccgtc tcgcagaccg gtttcaaaca
    10921 atatgtgcag gaagagcttg tactgaacgt ggaccagcgt ccgaatctcg atgtcgtgct
    10981 ccaggtgggc agcgtcagcg atagcatcac ggtggatgcc tcggcaccga tgattgaagc
    11041 tacacacgcc aacatcggcg gtgtcgtcga caaggtcaac gtacagcagc ttccactgaa
    11101 cgggcggcaa tttttgcaac tggcattgct attgcccggc acaagccccg cggccggagg
    11161 acaaaccgtg gcgcggggag gcggtccacg gaacgtgggt ttccagggcg gcgggaaccg
    11221 cgcgaccaac aatacattcc tggtggatgg ggtggattcg ttcggcttcc ggttcaagaa
    11281 tacgagcctg cggccgtcgg tgtcgtcgat ccaggaattc aagatcctgg agtcgcccta
    11341 cgatgctcaa tatggcgtcg cctcaggggt tcacgtcagt gtcatcacga gcagcgggac
    11401 caaccagttc cacggtgaac ttttcgagta tatccggaac gataagctgg acgcccgcaa
    11461 ctttttcgac cagcagaagc cgccctatag acaaaaccag ttcggcggcg ccatcggagg
    11521 acctgtgatc cggaacagga ccttcttttt tgggaactac gagggattcc gggcgcggcg
    11581 cgggcagacc aattccgctc aggttccctc gccggagcaa ctggcggggg actttcgcag
    11641 cgacccgcga gccatcatcg atccgctaag cggtctgcca tttccggaca agacgattcc
    11701 gcaaagccgc atctcggagg tgtccaaacg cttcttccag tttttcccgg ctcctaacag
    11761 cgcgctggcg gcgcccaact tcatcaactc ggcctccagc gaagtggtgg aagaccagtg
    11821 ggtgggcaag ctggatcaca ccttcagcga gaaatggcgt gtattcggcc gctacagcta
    11881 ctcgaaggtg gagagtttcg ttcccggaag tgtccccctg ttcggaacca tcaacgacat
    11941 gtatgttcac aacgccgtga tcgggagcac gctcattctg agcccgcgga cttttctgga
    12001 tgtgcgcatc ggctacaacc gtgaagacgc gatcaaccgg tccgaacaga tcggccagat
    12061 tccgagccgg gaatttggga ttccggggtt gtttgtgcct cccgaggtgg atggcgtgcc
    12121 ccaggtcgcg gtacaaggtt ttgccaccat cggcgatgcg atcaacagcc cggaaggccg
    12181 ggtggaaaat tccgaacagt tcatcgcgaa ttggacccgc gtgcagggac ggcacacgat
    12241 tcgcgcgggc ggcaacctct ggccgatcca gttgaacaag gttttcctct ccggcatcca
    12301 gaggggccag ttcaacttcc agaacattca tgcgcaggga accaccggac tgccggagtt
    12361 tctgctcggt ctgccgcaac aggcgcagcg caacatcgga ctggtacgga acgacgcccg
    12421 cactgtgttg ttcaatgcgt atgccagcga cgatgtaaga ctcagcagaa gacggacgct
    12481 gtcattcggg ctccggtatg aactgcggcc gtcgttcatc gacaaggaag accgccgtgc
    12541 tgttttcatt cccgagggcg aaggaaagtt cgtggtagcg ggcgacccca acaacggttt
    12601 caccgggagg atgaaccgat cgctctaccg cacgccggta aagaacttcg cgccgcgatt
    12661 cagcctggcc gccgatctga ccggggacgg caagacggtc ctgcggaccg gatacgggat
    12721 cttctacaac atggagatct acaactcgca attgcttgac gccctgaatc cgcccttcag
    12781 cattttccag atctaccagg cggaccctgg catcggcgtc attctgcgtt gggaaaatcc
    12841 gtttgacgcc acggacccgc gcgccggtgg tctgccttcc ggctacggca tcacgccctg
    12901 gttccgccag ggatacatgc agcagtggag cttcggtttg caacggcaac tggtggagaa
    12961 ccttgcgttg gatgtttcct atgtggcgaa caagggcacg gcgctcgacg gcgtccggct
    13021 gatcaatcaa ggggcgctgc ccggcgcgtc gaacgccgtg tacgtgcgcc agttccccaa
    13081 cttcggatat cacatcacgg ccgacagcca cggcgactcc atttaccact cgctccaaag
    13141 ccggatcaca cggcggtttt cgaaggggct gacgtttatg gcgtcctaca cgtggggaca
    13201 cgccatcgac aactcgaacg gagagggcaa cggcagtggt gggcagtttt tcgcgatgga
    13261 tgaccggaac ctgcgcctgg accgcggaag ctcggatttc gacacgcggc accgggcaac
    13321 cttcagcggc atctacagtt tgccgtttgg acgcggccgt ctgctctccg ggtggcagat
    13381 tgccgcgatg taccaggtgc aaaccgggtt tccgtccaca gtcggtcaat ccggtaatcg
    13441 aagcggcact ttcgctatcg gcgagcgcgc cgacattctt tgcgacggac ggctcggccg
    13501 cggagagcgg acacgcacgc ggtggttcga caccgcctgt tttgctccgt cgccgctcgg
    13561 aaagttcggg aactcctcgc gcggcgcgat tgtgcggccg ggacagaaca acctcgactt
    13621 ttcgatcatc aaggatacaa aggtgacgga gcggatgcag gtggaattcc gcgccgagtt
    13681 cttcaacctg ccgaaccaca cgcagttctc catgctgggg aatctcggta cgagtgtcac
    13741 aacacccgcg acgttcggcg tcattacggc ggcacaggac ccgcggatca ttcagttcgg
    13801 cttgaaactg aaatattgac gagcatctac gtccgccagc ggacgccggc atccaacgtt
    13861 ttccggcagt attcgaggct gtgttcgaga tccaccatct gctgctgttt gagtgcggcc
    13921 tgatactccg gcggaacctt tgttccggcg tcggcgatga tcatcatgcc ggaaaacggc
    13981 cgcccgcgct tggcaagggc aacgaagcgg gcgaattccg cggcgtccgt tttagggaag
    14041 gccgcccaga attcagattc caggtaaggc aggacgcgcg gaggccgccc ggtaataatc
    14101 tccagttgca tggaagcggc ggggcacaaa cggcggtgca attcgaggat gcgcttgaaa
    14161 tccagaatgc cgtcgccgac agcggtccat tgcatcgcgg cgcctcgcgg gtgctcgtag
    14221 acgacggagt cacggacatg agtggtgacg gtataggggc cgagcacctc catcgtaacg
    14281 accgggtctt ccaccaccga catagggttg ccggtatcga ggcacgcggc aacgtagtcc
    14341 ttcccggcct cttcgatgag agctttgagt tcgcgggcct gcaggtcgga atgattttcg
    14401 atagcgatct tcacgcccgc gtcggccgcg tgagagcggg cggcccgcag cacttccacg
    14461 gtcttctcaa tatgcgcttc gataggcagt gagccgaggc ggtccgccga gcttccgatg
    14521 aaacaccgca tggatgtcgc gccgacagcc ttcgctaccc gaagcccggt gcggatgtat
    14581 tccaccgggt ccccggtttt cggattccag cttttcgttg tcggacagat gcagccaatg
    14641 cccgcatcga tggagatgcc gtgcctggtg gccgcatctt tcaccttttg gagataagca
    14701 ggatcgaggc tctcatagtc cccgaggccg gagagttgga tggtgtcgag cttctgggcg
    14761 gctgcgaagt cgaggtgttg aagagctttc cagcggaagg cgcgaagaga atacgtatcg
    14821 tagccgagtt tgaggaagcc ggtattggcg gcggccggac gggacagcgc ggcggcagcg
    14881 gcggtctgga ggaacaggcg tcggttcatt ggaagagccc taactcacga gtgtaataat
    14941 acagccttga cgccgcttga aaaggtcgaa cacgagatac gccggtacga gcataccctg
    15001 ctgaagtttt cggcgcgaga gaagaaggac ggcagcgtgg aactcgtcat cgaattgaag
    15061 gaaccggccc caggagtgca cacctattat gccccgatac acccacggga catcgagaac
    15121 tcgcaattcc cgtggacgtt ccagcgctat ctgtacgatt gcctgcacga ttacctggtg
    15181 gagttgttcg tcaggacgcc gcagagccgt gaggcgcagc cgtgacggaa gccggacgga
    15241 ttctcgcgga ggaaattcag cgtgaagggc cgattccgtt tcgccgtttt atggaagtgg
    15301 cgctctatca tcccaaattc ggctactaca ccagcgggaa agacccgttc ggccgggccg
    15361 gcgacttcta caccgctgaa caaatacagc ctgtcttcgg aattctgatg gcacgataca
    15421 tccggtctct tctcgacgag atgggcgggg ggaccggcgt ggtcttggaa ctcggagcgg
    15481 gtcgtcagga gatgaagccc tttttgtcgg atctggaata cgttccagtg gaagccggga
    15541 aaggaacgat gccagcgcgc ttttccggag tggtgttcgc caacgagttc ttcgatgcgc
    15601 ttcctgtgga cgtcgcggtg ctgcgcgagg ggaagtaccg cgagatgctg gtggactttg
    15661 atggcaacgg ctttgtctgg cgggacggga atgctgtacg gcccgaggtg cgggagtatc
    15721 tcgaccggtt cgccgccgcg gaggaagagg gatgcattcg cgaggtgcat ctcgaatcgc
    15781 tcgcctgggt cgagaaaatc tccgccgcgc tcgaaaacgg atttctcatc gtgatcgact
    15841 acggctatac ccgccgggaa ctgatgcgat tcccacgcgg cacactgatg agctaccgga
    15901 ggcataccgc gtctgaggac gtattgaaag atccggggga gagggacatc acctcccatg
    15961 tgttttttac caccctagag gacgtggcgg catcgcatgg gttccgtacg gccgcgttcg
    16021 aatcgttgtc gcgcacgctg ttgcgggtgg gagaagcgga ctggttcgct tccgtgctgg
    16081 agggagtcag tgagagcgaa cagctccgga ggaggctaca gttgaaaacg ctgcttttcg
    16141 gaatggggga gacgttccgg actcttgtac tcggcagggg gccaaaataa aagcggcccc
    16201 gactcaccgg ggccgctcta actttgactg ccgtcctcgt gggcggctcg ggaactactt
    16261 cttccctttt ttcgccgggg ccttcttctt tgcaggggcc ttcgcggctt tcttggttgc
    16321 gttcttcatc gattgattct ccctaaacat caaatttgcg atctcacgtg agatcgcagc
    16381 gtgattcata tataaatttg tttgcatcga aagtcaagaa aaaagtgaac gtaacgtgca
    16441 aggggaattg ctgaaacgtg acatataaga attgttttgg ccgaaattca cgggaacaaa
    16501 ggcgcgcgag gcccttgccg aaggctctgc gggcgggtta gcatcacagc agatgaggtc
    16561 gcgagcgttc ctgatgggag tggcggttgt gctgctgctg tccggttgcg gaaagaagaa
    16621 accgcaggcc gcgattcccc ggccggctgg atggcaggaa aagggaatcg caagctggta
    16681 cggaaatccc tatcacgggc gggccgccgc caatggtgag atctatgaca tggagaagct
    16741 cacggcggcg caccggactc tgcctttcga tacgtgggtc cgggttcgga atctctccaa
    16801 cggaaagacg gtggaggtgc gcatcacgga tcgcggtccg tttgtcggcg atcgtatcat
    16861 tgacctctcc cgcgccgccg ccagaaatat cgacatgatc ggccccggca tcgtaaacgt
    16921 gcgattgcag gtgatcgcac ccccacggta tgtgcagccg gtggattatt tcgccgttca
    16981 ggtaggtgcg tttcaggaac ggaagcgagc ggagcggctg cgccaggaga tggagaaacg
    17041 gtacgggccg gcccgcgtcg tgatccgcga cgggaagcct ccgctgtggc gcgtgctggt
    17101 agggagggca ccgacactcg aagcagccaa cggcatcgcc gagcagctcc gcgccgcgcg
    17161 gcactctgcc tttgtggtcc gcctggacga tcccgcgatg taggcgattc gccttaaaac
    17221 ccttaagaat tttccgggtg tcactacacc atgtcttata tgggaatttc cggctgcccc
    17281 cctatctgcg cggaccgcga cctggtcggc gacccgattg gagtcaggaa cacgcttgcc
    17341 gaaatcgagc gcgccggccg caacaggctt ccggtgcttt tgctcggcga aaccggaacg
    17401 ggtaaaggga ttatcgcccg tcggattcat gcggttcgag gaaccgggcc gttcgtcacc
    17461 atcgactgct ctgccctggt gggcgagttg atggagagtg aattattcgg ccacgtgaaa
    17521 ggatcgttca ccggagccgt ctcgggaaaa atcggattga ttgagatggc cgatggcggg
    17581 acggcgtttc tggatgagat cggagagttg ccgctggagc tgcaggctaa actattgcgg
    17641 gtaatccagg agaaggagtt ccgtccggtc ggatcgacgc aaatccgccg ttcggatttc
    17701 cgggtgatcg ccgccacttt gagggacctt gccacggacg tggcggaaaa aaagttccgc
    17761 agcgacctct acttccgtct gaaggggttg accatccgcc tgaaaccgct tcgcgagcgg
    17821 agagaagata ttccggtgct ggtgaatcac ttcctgcgcc ggtacggaaa caatcactcc
    17881 atcacggcgg aagtgatgga tgccctggtg gcatacgact ggcccggcaa cgtgcgggaa
    17941 gtggagctct gcgttcagca catgatcgca ctgagtccgg agccgctgat tcatacggcc
    18001 gcactgacct caatggtgtt gaacgacatg cggcaaagga ggactgcata ccgggttcac
    18061 ggcgcggcag cgggtgtgga aacgttgcag cgggaattcc tgccggcctc ccctgtgccg
    18121 gattctatag gccccgccgt gtttggatcg gaagtgatac ctatccctga attggagcgg
    18181 agggccatcc tccacgccct ggagcacacc aagggcgacc gcacgatggc ggcgcacctg
    18241 cttcaaatcg ggcgcaccac cctttacagg aagatcagag attacggcct agactgatcc
    18301 cgtccttcgg cctccctgtt tcagaatccg gatacggccg ttcaccgtgc tgagattgag
    18361 ctgtcttccg ccggagccga tggtgcctgt cagcttcttg ccgacgaagc cgctcacggt
    18421 aagggggaag tcggtctcaa tacctccgtt caacgtgttg gcgttgacgg cggtattggt
    18481 ggtttcgggc agggtgacgc tgatggcgcc gttcactgtc ttgaactcgc gggcctccgt
    18541 ccaattcgca tcgccgcaga ccacgtcgat agctccgttg acagtacgcg cctgcgcatt
    18601 cccgttggtg gagatggcca cctggccgtt caccgtgtaa gcttcggcat cgccctgcaa
    18661 ctggcgtgca tcgattttgc cgttcacggt ttttccgacg aacttgacgc cggccggcac
    18721 acggaccgtg aagtgcacct gcacatcgtt gttcgatacg ttgttgcggc cactgttgcc
    18781 gggcttacat tcattcggct gggcatcctt actgggataa accgcgcaga tggtgactcc
    18841 tccggagtgg ggtacgacca cgatttcgac gctggcaggg tcgctccggt tggcggactt
    18901 ccgcgccgat acctctgccc gggagccgga cgccgcctcc gccgcgatac cgccgttcac
    18961 gcccttgatc tccaatacct ggcctttgcc gatctcaccg tcccaatgga attggctgga
    19021 ctcgacggca aacagcccgg agccgagcgc caaacatacg agcgcacctg gaataaggtt
    19081 tttcatcctg cctcctgtat ccgcaatacg aaatccggaa tccgccggtt cctcaaatgt
    19141 tccaggtgta gacgtcggcg ggggaaaaag gttagctaga ctggttgaat ggctaagcaa
    19201 tatccgtccg cgccggcgat ggcgatcgaa cccaacaaga gctacacggc cgtcttcgaa
    19261 acatccaaag gcaacattga atgccagttg tttgcagaag acgctcccgt cacggtgaac
    19321 aacttcgttt tcctggcgcg ggagaagttc tatgacggga cggtgtttca ccgcgtcatc
    19381 aaggatttca tgattcaggg tggcgatccg acggcgacgg gaacgggagg gccggggtac
    19441 cgcttcgcgg acgaagtgaa gaacaatccg cgcaagcacc aggttgggtc gctatcgatg
    19501 gcgaatgccg gtcccaacac gaacggcagc cagtttttta tcacgcatgt ggtaacccag
    19561 tggctggacg ggaaacatac ggtcttcggc caggtgacgt ccgggcagga tgtcgtgaac
    19621 gccattcaac agggcgatca gctcaagagt gtcacgattc aggaatccgc gtaaggaccc
    19681 ggggcgccgc tactgctggc gcccgtcttc caggtaggtg taaccccgga gcccctcttc
    19741 gtagaaccgc aagagacggg cgagttcctg atagccgatc ctgccttcac gcaaagccgt
    19801 ttccgcgtcg cgccggaact cctcgaggag cgtctgtgcg ctgaactgca cgtagttgag
    19861 cacctcgcgc acggtgtcac cctggatcac ggcgtcgagc accacctcgc ccgatgcgtc
    19921 caggctcacg tggacggcgt tggtgtcgcc gaacaggttg tgcagatcgc cgagaatctc
    19981 ctggtaagcg ccgacgagaa atgcacccag gtaataatgc ttgccgttga aagtatgcag
    20041 cggcagcgtt ttcttgacgt cgcggcggtc gatgaactga tcgattttgc cgtccgagtc
    20101 gcacgaaata tcgcccagga tggcattcct ggtgggcggc tcccccagac ggtggatggg
    20161 catgatcgga aaaagttgtt tgacggccca actgtccggc atgctctgaa acagcgaaaa
    20221 gttgcagaag tacgtatcgg agagcatggc ctccaaccct tcgagctcct ccggaacgta
    20281 ctccatatcc ttgatcattt tctgaatctt ccggccgatg ccccaataga ggttttccgc
    20341 caggctgcgc tgttcgagcg gcaggtatcc gaggctgaag agattgagcg ccgcatccag
    20401 ggcctgttgt gcgtcgtgga aaccttcgag caggttcttc atgctgagcg agcggtaggt
    20461 ctggtgaaga tccaccagcg gctgctcggc ttcttcgggc aagtcctgcg gcacgtcctc
    20521 ttcgccgagc ccggtgacac cgaggacgtt gaataccaga acgctatggt aggccgcgac
    20581 cgcccggccg ctctctgtaa cgattgtcgg atgcgggacc tccgcttcgt cgcagatgtt
    20641 ttgaatatga tagacgacgt cgttggcgta ttcctgcagc gtataattca cgctcgattc
    20701 gaaatcggtt tgggagccat cgtaatcgat gcccagaccg ccgccaacgt ccatgtactt
    20761 caaccccgct ccggcgcgtt tcaattccac gtacacgcgc gccgcttcgg tgaccgcgcc
    20821 cttgatatgg cggatattgg taatctggct gcccaagtgg aaatgaagca actccaggca
    20881 gtcttccatt ccgagttcct tgagccgttg gagcactcgt agagcctcgg aaacggtgag
    20941 gccgaatttc gaacggtaac cgccggagga tttccatttg ccggcgcccc ggctcgccag
    21001 tttcacgcgc aaaccgatgg cgggacgcac accaacgttc tgcgcatgct ttacgatcag
    21061 gtcgagctcg gtaaatttct cgattacggg agtgatctgc cggcccattt tccgggccag
    21121 catcaccgtc tcgatgtact cgtcgtcttt aaaaccgtta cagatgacgg gggtctcgtt
    21181 atcggcaaca gccagcacgg cgagtaattc gggtttggag cccgcttcca gtccgaaacc
    21241 gtagggccgc ccgaagcgga acacctcctc cactacgtgc ctttgctggt ttaccttgat
    21301 cgggtaaacg caaacgtaat cgcctttgta gccatgttcg gtaatggagc tctggaatgc
    21361 ctggtacagg tctcccaggc ggtgcttcag aatatcggcg aaacggatca ggatgggaac
    21421 ggagataccg cggagcacca gcttgtcgac cagttctttc agatccgttc gccggttcac
    21481 atccttgtcc gggtgtaccc agaggtggcc attctcaccg acggagaaat agcctttgcc
    21541 ccagctcgcc acatcgtaga gttcggcggc gtccgtcacg gtccagcgct cgctcggctc
    21601 ccaggtggtg gccccctcag acagtttcaa cgtcaattac ttcaattcct cccgaaagct
    21661 tcagtgtcct tcgaatccca ccaaacagca agcgaaagtt ctttcaccat tcctgagcgc
    21721 ttgttcacac cgaagtgagt gctggcgggg aatcgaaagg ctgccagatt aagacgccgc
    21781 ggagcgggaa ttggttccca attaccggaa atcctcgacc agcatgatat ccgtatcgct
    21841 ctggtctacc tgcgtgtaga gaaaatgacg gccgtcgggt gaaaccgaga ggccggcatt
    21901 gaacggccgt ttctcgatcg gcatgacggt gctctcccgc atggtcttca aatcgagcag
    21961 gcggaggtag tagcgaatgc caatatcctg aatctctcgc cgttccagaa aataaacgcc
    22021 ccgcgggacg atagcccaat atccccagaa gcccgcgcgc aagctctcca gcacgggttc
    22081 ttcgtccccg ccggcgatgg gtacccgcca cagcccgggt gccctcgggg atttcgcata
    22141 ataaatgtac ttgccgtccg gcgactcgat gccataaaaa ccgccgtttt tggtgacctg
    22201 tatccccgca ccgccttcgg ccggtatttt ccagagctgg taggtgcccg tccgattgga
    22261 ggcgaagtag atccagcggc cgtcattcga atacgtcgga acgacgtcgt ccgaaggttc
    22321 ggttgtcatc cggcgtggag ttccaccttc cgcgctaacg atataaacat ccggattggc
    22381 attcggccgt gaatcgaaag ctatagtttt accgtcggga gaccagtgag gactgcccgt
    22441 cgcagcaccc tcgaaatagg taagccgggt agggaagttc ccgtccttgt cgcacagcca
    22501 gatttcggga gtgccggagc ggttcgatgc gaatacaatg cgccggccat cgggcgaata
    22561 ctgcggaccc tgctcgtagc gggtcgatga aatcaagcgc gtccctttgc ccgctgctcc
    22621 ggcgctgcct gtcaggtcga agcgccacac gttcgtatcc aaaatcgagc gggtataagc
    22681 cagtcgtttg ccctggggcg agatggcaat aaaggacgcg ttttcaccaa tgccgatgat
    22741 gcgctccggc gcgccgcctt ttggagagat cttccagaga cgcgaactgc ccgcccgagc
    22801 ggaggaaaaa taaatcagac catcggacgg gttccatgcc aggccataga tgcgccggtt
    22861 gtcgaacgtc aggcgttccg gttccccgcc ggcgacgggc acaacgtaaa tgtcctgcac
    22921 ggccagcgct cccgtccgga cgaaggccag catccggccg tcggcggaga actgggggct
    22981 gctgtccccg taagtcttat ccggtggatt cgtgagtttg cggcgctcgc cggtttcgat
    23041 gttgtgcaga aagatgctca tcggttcctg ctgcgcggtc ttatccacca gggctaccgt
    23101 gttgctgtcg ggggaccagc ttgcataggg agcgtcgacg cccgtccgct gcgggttcgc
    23161 gtccgcgagt ttgcgttcca ggccgcctag gggtggaatc aggtagaacg cggcagagtc
    23221 gctctcctgc cgcaggaaga tgatctggcg gccatcgggc gaccacgccg gactgaaatc
    23281 ggtggcgggg ctcgaagtaa gccgcaacgg agcgcccgcc tccaccactt ttacgtagat
    23341 gtcccagttt tcttcggttt cgctattcca ggtgaatgcg acctggctgc cgtccggcga
    23401 aaagctgggc tggctctcgt tgcccgggta cgcggtgagc ggagtcgcct tcaaagcgat
    23461 gggctcggtt cttgccgaac tccggctgtt gaaataccac gaagccagtg cgatcatggc
    23521 cgccacgctt acaatcaaca gcggcaggcg ggaccgtccg cgaagagaac gcgagccaac
    23581 ttccgccggt gcggtggtac gcccggaatc ggcgtcccgc ttggtacgtt tcaaatcggc
    23641 ggtgagatcc gaagcagtct ggtatcgcag ttcagcgtcc ttctccagcg ccttcagcac
    23701 aatccgatcc aactccggcg gaatcgaccc gtttaccttg gagggcggat cgggatgctt
    23761 gttcaacaac gcactgaaga cagccgcggt agtcggtccg ggaaacgcgc ggcgacccgt
    23821 cgccatctcg tacaacacgc tcccaagact gaagatgtcg gtgcggtggt ccacatcctg
    23881 tccgagcgct tgttcaggac tcatgtactg taccgtcccc atcacgatgc cggcatcggt
    23941 gagacgaccg ctgacagtct ccgtctgaag cgtgtggtcg ggcttgcgga ctttcgccag
    24001 cccgaagtcg agaactttcg cctgcccccg cggcgtgatc atcacgttgg ccggtttgat
    24061 gtcgcggtgt gtgatgccct tggagtgcgc ttcggcgagg gcttcggcaa tttgcgtgcc
    24121 gatctcgatg atgtcgctcg tggggagcgg acggccgttc aacctggcgg cgagggtctg
    24181 cccctcgacg tactccattg ccatgtagtg gacgccgtcg gcttcgtcaa tatcgtaaat
    24241 gtgggcgatg ttcggatggc tgagaacggc gacggccttg gcctcctgga cgaaccgtcg
    24301 cattcgctcg gaatccgcgg cgatgcgttc gggaaggatc ttgagcgcta ccaggcgttc
    24361 cagccgggtg tcttcggcga gatagacctc ccccatgccg cctgcgccga ggcgcgataa
    24421 aacccgataa tgcgcaatag cggcaccggg ggccatttta tgtagtattc cgagtttacc
    24481 attcagggca ttcctgcctg gtgcaggtgt agttctatac cggtcttgcg gcatccctga
    24541 gacattgtgg aagccagcag ttcggcgatc gtacggcgtg tcaccgggtg cctcaagccg
    24601 ggagcgagtt ccgccaaagg ttccagaacg aatcgccgct ccgtcatgcg gggatgcggc
    24661 agcgtcagat cgtgagtgtt cacgatcgcg gccccataga gcagaatatc gatgtcgatg
    24721 gtccggggtc ctttgtcaac cagccgtttg cggcccagcc ggcgttcaag tttcgaagtc
    24781 ctggcgagaa gttgccgtgg cagcaggctg gtccgggcct cgatcacggt gttcaggaaa
    24841 cagggctgat ccagcttgtc gaccgggtcg gtctcaaaaa cagaggagat gcgcaatacc
    24901 tgcaagccgg cggagtccag gcactcgaca gcccgttgca ggtgccgctc acggtctccg
    24961 aggtttgacc cgagggagag gtaaacggta gtcatcgaac tagaagaggc tgcgctgcaa
    25021 acctcgcatg tggcggggaa tgttgaaatg atcgaaggcg tggtcggtgg ccacgcggcc
    25081 gcgcggcgtc cggtcgagaa atccaagctg catcaggtac ggttcgtaaa cctcctcgat
    25141 ggtggcgggc tcttcatcga tggaagccgc gatcgtattc aaaccgacgg gaccgccgct
    25201 gtacttttcg atcactgtca gcatgatttt ctggtcgatt tcatccagac cgaagcgatc
    25261 cacctccagg agatcgagcg ccgtagtggc gatgccgtga tcgatatgtc cttcggcgcg
    25321 gacctgggca taatcgcgaa cgcgccggag caggcggttc gcgattcgcg gtgtgccgcg
    25381 gctgcggcgc gcgatctcac gggcgccctc ttcatcgatg gccacatcca gcagtccggc
    25441 ggagcgcagc acgatgcgct ccaactcagc cacatcgtaa tgattcagcc gcagaaccag
    25501 accgaagcgg cctcgcaggg gagcgctgac aagcccctgc cgcgtcgttg cgccaatcgc
    25561 cgtgaagcgg ggcatcggaa gagagtgcgt gcgggcgccg gggccggctc cgatcaggat
    25621 gtctacgcgg aaatcttcga gcgccgagta cagcatctct tccacgtcgg gaaggaggcg
    25681 gtgaatctcg tcgataaaga acacctgccg ggcccggatg ttgctgagga tccccgtcag
    25741 gtccagtttc ttctgaagaa tcgggccgga ggtctgttca aagcccgcct ccaactcgga
    25801 cgcgatgatg gtggcgagcg tcgttttccc caagcccggc ggaccatata agaggacgtg
    25861 atccatcgct tcgtcacgcc gccgcgcggc ttcgatcgcg atcgcgaggt tctctttcag
    25921 tttggcttgt ccggtgaagt cgtccaaccg gcgcgggcga agaccggctt cgaactgccg
    25981 ctcttcgtcg cgcggagcgg gcgacacgat acccttttca cgcatgcttt atctgccggc
    26041 ttagaccaat ttagcgcacc agttccagcg cacgccggaa caggggctca aattcggccg
    26101 gagcgcccgc cgctttcgct ttccggagcg ccgtttcggc ggcgccgcgg ttgcagccga
    26161 gattcaacag agcggaaagt atatcttctt ccacaggttc gagcgcgggc tgcttctgtg
    26221 cagggatccc ggcggcgccg gcgccgattc cagctacttt gtctcgcaac tccagcacca
    26281 ttctttccgc cgtctttttt ccgatgccgg gaatgcggac cagatgctcc aactgagcgc
    26341 cacgaatcgc ggtgatcagg tcggaagcgg gcaggcccga aaggatggtg atagccagcc
    26401 gcgggccaat gccgctgacg ccgatcagct tttcgaagag tgacttctca tccatggtca
    26461 gaaacccgaa aagcaaaagg gcatcttccc ggacatgagt gtggatgcgc aggcgaacct
    26521 cggaattggt atccggcaag gaagagaatg tggaaacggg gatggtgacg tcatagccca
    26581 cgcccgcgac gtccaccaca acctggttcg ggtgcttttc gagcagcgtc ccccgcaggt
    26641 aggcaatcat agatcaacat tatctccgat gatgcctacc gtctgagcac ggcagccgcc
    26701 ggagccaatt cggcgggtag attcccggat acgctcctag agcttctggg cgccctcgac
    26761 cttggccaga ttccggtcga acgtacccat ggggagatgt ccagccttgc gggccaaatg
    26821 aaacatcagg caatccgaaa agcccagcgc gggcttgacg cggaacagcg ccagcgccgc
    26881 ggctacggtc tccggctcct gtagcaccag gtcccggtga ttgagcaaca tctcaattgc
    26941 cttcgcctga tctttcgggc ccagttcata gacggcggaa agcacccaga tcgcctcggc
    27001 cagcgcgaga acagaaaccc aagcaccgtt ttcgatgaat gcctcggccg aggcggcttg
    27061 gcggaggtcg tcgcgggtga tcaggcgcac gaggacgttc gtgtcaaccg cgcgcatgac
    27121 gtttccagac gtagttgcgg ataccgctct tcagttcagc caagcctcgt ttctttggcg
    27181 tccgcgctcc gaacaacgcc cgatgaatgt cctcggaggt gaaccgtccc gcgcgccgga
    27241 cgacaatctt gccgtcctcg tcgtcccatt ccacgatcga gccgggaccg gctccaagct
    27301 ttcgccgcac gtccgcgggg atcgatattt gcccctgcgc cgtaattttc gagcgcgcga
    27361 tagccatgct tttatattac cagggtaatg gacggatcac ggcgggccgg ccgggagcgg
    27421 ttcccttgaa tacgtaagcg gtaaactgtg ccaaatttga tcgtaggcat ggctcgcagg
    27481 ctatttttcg tgaatgaggt gcactcggac cgcgcggaga tcagcgggga ggacgcgaag
    27541 cacctgacgc gcgtgctgcg cgtcgagcgg ggccagcggt atgaaatatc ggacaaccgc
    27601 acgctgtacc tggccgaaat tgaaacggcg cgcaaggagc atgtcgtgtt ccggattatc
    27661 gaacggctgg aatacacggc tcccaccctc acgatttcgc tctgtcctgc attgatccgg
    27721 ttcgagcgct tcgagtggat cctggagaag gccaccgaac tcggcgtggc ggcgattacg
    27781 ccgtttgcgg ctgtgcgttc ggagaaaggc ttggaccgcg cgtccgccaa gagaatggcc
    27841 cggtggcgca agatcgtgct cgaaagcagc cagcagtcgc ggcggccggt gatgcctgtg
    27901 ctccatgaag cgataccgtt ccaggtggtg cttcgagcgg aaacgcagcg gaagctgctg
    27961 ctcgatgaag cgcgggaggc tccgcccatc cgctccgtgc tgaccgcgtc ggaatccatt
    28021 gcggtactaa cgggaccgga aggtggctgg accgatgccg agcgcgctgg ggccatcgcg
    28081 gcaggttgga cgcccgtctc gctgggtccg aacatcctcc gcgccgagac ggcggcagtt
    28141 gcggcgctgg cggttctgtt ttgcgcatcc gacagctggc ggtaaagtta caattcgaga
    28201 tatgaacttt tccagaaggc agtttctcgg cgccgccggg gccgccgcgc tctccaccgc
    28261 gcgcagttat tcgcaagtcg ccggcgcggg ggagcgattg cgtattgggg tgattggttg
    28321 cggaggcatg gcgaacggcc atatgcgcac gctgatgaag atgcgcgaat ccgacaacgt
    28381 cgaaatcacc gccgtctgcg acatttacga caaacggttg gatgccgccg cgcaactgac
    28441 cggtgcgaag cctgtaaagg actaccgcac gctgctacag gccaaggaca ttgattacgt
    28501 tctgattgcg actcccgagc actggcatta tcagatgacg atggacgcgg cggatgcggg
    28561 caagcatatc tactgcgaga agcccatgac gcacacggtc gaggagtccc ggaaggttat
    28621 ggacaagatc accaagaccg ggctgaagat gcaagtgggt gtacagggaa tgtcggacga
    28681 gtcctacgcg acggcgtacg agtatatgac caagggcgcc cttggaaaag tggtgcttgc
    28741 gcagatcgac tactcccgca attacaaagg tgatttctgg gcctatccga tcgacgaagg
    28801 cgtgcggccc ggcgagaatc tgaactggaa tgcctggctt ggttcggccc cgaagcaccc
    28861 ctgggacccc gaacgttatt tccgctggag gcgctactgg gattactcgg gagggatcgc
    28921 ttccgacctg ttcgtgcatc gcgtaacacg tatcatcaag gcgctcgggc tgaagttccc
    28981 cagcatgggt gtggcgaccg gaggtaagt
//
LOCUS       DASTMK010000150         6050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_199655, whole genome shotgun sequence.
ACCESSION   DASTMK010000150 DASTMK010000000
VERSION     DASTMK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199655"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..886)
                     /locus_tag="VFQ79_06385"
     CDS             complement(8..886)
                     /locus_tag="VFQ79_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU0139317.1"
                     /translation="MDSGRPDEESNLALKEKIREDLKELREDLKEPQKTQLWKSVFRV
                     KHERADPRTRALGVLTNVFLHLHPARINRDAVRYSYTWGMGGITFYLFIVLTFTGVLL
                     MFYYHPTKIQAFRDILFLEHDVPFGKLLRNMHRWAAHLMIITLWLHMFRVVLTGSYKK
                     PREFNWCVGVILLVLTLLLSFTGYLLPDDQLGFWAVTVGTNMARATPLLGSEGPLGPE
                     LGMTPFNDVRFALLGGSIVDANALLRAYIWHCIAIPLIAGVFMGVHFWRVRKDGGISG
                     PAPVMLESEIKPVLKR"
     gene            complement(994..1608)
                     /locus_tag="VFQ79_06390"
     CDS             complement(994..1608)
                     /locus_tag="VFQ79_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU0139318.1"
                     /translation="METTRSPGLGGETIPKPPSKEKVTAKPAVVEAVGPAKPQSDGKE
                     PLTLRRRLVWTAIGGFLITNFLMFLRFFFPRTLFEPSSRFRIGYPGDFGLGVDERFKQ
                     SHRIWVVRQPDKLFVVYAKCTHLGCTPNWVEPENKYKCPCHGSGYDMEGRNFEGPAPR
                     PMDRAHVEFDADGQIVVDVARLYACPPGAPCQFETDPNSYLVVS"
     gene            complement(1756..2148)
                     /locus_tag="VFQ79_06395"
     CDS             complement(1756..2148)
                     /locus_tag="VFQ79_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139319.1"
                     /translation="MTEKAAELVRLLEAELDLIESGAYEPSAGKPTEDKPMFYHSLIC
                     INHWFVPGHEPECHDDCALLDAVPDKRKSEALPCHHIPLNEAGDTVKSLEEKGQHERV
                     KEEVANWLRNAIRQLKMEEKPVRSDVKY"
     gene            complement(2166..4322)
                     /locus_tag="VFQ79_06400"
     CDS             complement(2166..4322)
                     /locus_tag="VFQ79_06400"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEU0139320.1"
                     /translation="MKITVFYVGSSLLAPLKNAEREINEQYRLGLAVAVHNCTLPVPD
                     AEWPAVERDIDDAEIVLIIHVTDNDNAARIAAALDRCRSRHRAVIAINCMRDLMVRTR
                     LGKLDGMKLFHLWKEKERSVIYRAVRDAGSWMGSYARARNRDGKSAGGHKHSQLLKQM
                     PSILRLAPSVGVLRDVKHYLTAFCYLLQPTPGNIRSLLLYTIRHYIPEQARGIRRIEA
                     PENRPSTGIYHPDAASLFSSFEEYRAWYEARPFENDCHPRMDVNRAVGLLLTRPQIVS
                     GACRHYDYLIRLLESEGLPVVPVLSTFMDNREACQEFLVDAQTNTPRVAQLVSLTGFS
                     FVGGPAMNDSEAAVEYLKVLNRPFRSIVSLEMQRIEQWTESFIGLNPVQTTMQVAIPE
                     IDGATEPFVFGGLAAGKDEPEALEERCERIVRRLVRWDRLRLVPRSELRLAFIVYCFP
                     PDKGNLGTAAELDVFPSVWDVLRRLQAEGYDVDVPETPDALRSLLLGANGNIARAGEH
                     LAAVAYRMPVEEYYRSCPYVREIEEEWGPAPGRINAHGRDLLIHGVQLKNVFLGVQPT
                     FGYEGDPMRMMMAKNGTPHHGFMAFYLYLENIFRADALIHVGTHGALEFMPGKQAGLS
                     GRCWPDRLIREFPNIYIYSVNNPSEGSIAKRRSYAELVSYLTPPIENAGLYRDLAALK
                     ELLSNYRQVQDETQKEQLFVSIEEKARHLNLELKVS"
     gene            complement(4319..>6050)
                     /locus_tag="VFQ79_06405"
     CDS             complement(4319..>6050)
                     /locus_tag="VFQ79_06405"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEU0139321.1"
                     /translation="ERDRIVLRLYEQLLEIEQRLIPTGMHVFGKPGAQTERGDMLRMV
                     ASFDRPEMGFRSLPGQIAASLGFPEYPVLMEESATSGERLLQRERVDAILRSAIEKLL
                     AEGGGIEEAASLLAREVSIPTEDSTRMLTFLSDLDRQLGINHETDELMRALRGEYIEP
                     GPGGDLVQNPKILPTGRNTHAVNPYAVPSRVAFQKSQETIGRLLNRCLEERGRYPETI
                     GMVIWGMDNIKTQGEGVAQALLLMGVVPKRDGMNRVTDVEVIPPETLGRPRIDVVFVL
                     SGIFRDLFGTTMELLDKAARKVASLDEPPEVNFLRKHVQQAQQEGMSWCDAATRLFSN
                     APGNYGTNVNFMVIGGHWDRTEQIGDLFVTRKCFAYGRGLDGDPARTLMDKALSRVEV
                     TYQNLDNAEIGITDVDHYFEYLGGLTQAVEQRTGERPATYVCDSLSPLVKIRSLNETV
                     RLEVRTKLLNPKWYGGMLKHGFSGVAEIEHHVSNTFGWSATAGAVDDWIYSEIANTYV
                     VDEAMRQRLAELNPHATRSLAQRLLEAHGRGFWAAQQSVLDHLHNALDKLEDHLEGVN
                     GQASRASQGS"
BASE COUNT         1269 a         1795 c         1691 g         1295 t
ORIGIN      
        1 cgtgctctta ccgcttcagc accggtttga tctcggactc cagcatcacc ggcgccggcc
       61 cggaaatccc cccatcttta cgtacccgcc agaagtggac gcccataaac acaccggcaa
      121 tcagcgggat tgcaatacag tgccagatat aagcccgaag cagcgcgttc gcatcgacga
      181 tggagccccc cagtaatgcg aagcggacat cgttgaatgg cgtcattccc agttccggtc
      241 ccaacggccc ttcgctgccc agaagcggag tggcccgcgc catgttcgtt ccaacggtta
      301 cggcccagaa accaagctgg tcatccggta aaagatagcc tgtaaatgag agcagaagag
      361 taagaaccag cagaatgacg cccacgcacc agttgaactc gcgcggcttt ttgtacgatc
      421 cggtgagaac cacacggaac atgtggagcc agagcgtgat gatcatcagg tgcgcagccc
      481 agcggtgcat gttacgcagc aacttaccga agggcacatc gtgttccaga aacagaatgt
      541 cccggaacgc ctgaattttt gtaggatgat aatagaacat cagtaatacg ccggtaaatg
      601 ttagaacgat aaagagatag aaggtgattc ctcccattcc ccaggtgtaa ctgtaacgga
      661 cggcgtcacg gttgatccgg gcaggatgaa gatgcagaaa aacgttggtg agaacgccga
      721 gtgcccgggt gcgcgggtcg gcccgctcgt gtttgacgcg aaagaccgat ttccagagct
      781 gcgttttctg cggctctttc agatcttcgc gcagctcttt caagtcttcg cggatcttct
      841 ctttgagagc gagattactc tcttcgtcgg gacgcccaga gtccatcttc gtgtctcccc
      901 agaaagacag ttcggagtcc gctccctcac ggtcgcggct cagaaacgga gccgcgcgcg
      961 ccagcaagcg gtctttcgtt acccggtaag tgactacgaa acgaccaaat atgaattagg
     1021 gtccgtttca aactgacacg gcgcgcccgg aggacatgca tataaccggg ctacatcgac
     1081 tacgatctgg ccatccgcgt cgaactcgac atgtgcgcgg tccatcggcc ggggagcggg
     1141 accctcgaag tttctccctt ccatgtcata tccgcttcca tgacacgggc atttgtactt
     1201 gttttcgggc tccacccagt tcggagtgca tcccaggtgg gtgcacttcg catagactac
     1261 aaacaactta tccggttgcc gcaccaccca gatacgatgg gattgtttaa accgttcgtc
     1321 cacaccaaga ccaaaatccc ccggatagcc gatacgaaac ctggaactgg gctcgaaaag
     1381 agtgcgcggg aagaagaagc gcaggaacat caggaagttg gttatcagaa atccgccaat
     1441 cgccgtccag accagacggc gccgcaacgt cagtggttcc tttccgtctg actgcggttt
     1501 ggctggcccc acagcttcga ccactgcggg tttagcggtt actttttctt tcgaaggcgg
     1561 ctttggaatc gtctcgccac ccagacccgg agaacgcgtg gtctccatgc cttgccatat
     1621 ccttcccagt agattggctt gccgccacat tatattggcg aatgaacttt ttgtcaacaa
     1681 gaagaatctg tgcgccggcg gcgatagaat ttcaggcgcg cgagaactgc ccgaagcggc
     1741 gcagaacctc gcaggtcagt atttcacatc cgaacgaacc ggcttttctt ccattttgag
     1801 ctggcgaata gcattgcgca gccagtttgc cacttcttcc ttgacacgct catgctgccc
     1861 cttttcttcc agggatttca ccgtgtcgcc cgcttcgttt aggggaatat gatgacaggg
     1921 cagcgcctcc gacttgcgct tgtcagggac ggcatcgagt agcgcgcagt cgtcgtgaca
     1981 ttccggctca tgtccgggaa cgaaccagtg attgatgcaa atcagggagt ggtagaacat
     2041 cggcttgtct tcggttggct tccctgccga aggctcgtaa gctccacttt cgatcaagtc
     2101 gagttcggct tcgagcagcc tgaccagctc agcagccttt tctgtcatct ggactcctcc
     2161 tttacttacg atactttgag ttccaggttg agatggcggg ctttttcttc gatggacacg
     2221 aagagctgct ccttttgagt ctcatcctga acctgccggt agttggaaag taattccttt
     2281 agcgcggcca ggtcccgata caggcctgcg ttctcaatcg gcggtgtaag atacgacact
     2341 aactcagcat aagaccggcg tttggcgata ctgccttccg aagggttgtt cacactgtag
     2401 atgtagatat tcggaaactc cctgataagg cggtcgggcc agcaacggcc cgatagcccc
     2461 gcctgtttgc caggcatgaa ttccaatgcc ccgtgcgtgc ccacatggat aagggcgtcc
     2521 gcgcggaaga tgttttccag atacaaataa aaagccatga aaccatggtg aggcgttccg
     2581 ttctttgcca tcatcatccg catcggatcg ccttcgtaac cgaaggtcgg ctgcacgccc
     2641 aaaaaaacat ttttcaactg cacgccgtgg atcagaagat cgcggccgtg agcgttgatg
     2701 cggcccggtg caggtcccca ctcctcttca atttcgcgca cataaggaca ggaacgataa
     2761 tactcctcga caggcatgcg ataggccacc gccgccaggt gttcacctgc ccgagcgata
     2821 ttgccgtttg cgcccagcag gagactgcga agcgcatctg gggtttccgg aacatcgacg
     2881 tcgtatccct cggcctgaag gcgccggaga acatcccaga cgctgggaaa cacgtctaac
     2941 tccgccgctg ttcccaggtt gcccttgtcc ggaggaaaac agtaaacgat gaatgccagg
     3001 cggagttcgg aacgcggcac aaggcgcagc cggtcccatc gaaccaggcg gcgaacgatc
     3061 cgttcgcagc gttcctcgag cgcctccggt tcgtctttac cggccgcgag cccaccgaat
     3121 acgaagggct ccgttgcgcc gtcgatttcg gggatagcca cctgcatggt cgtttgtacc
     3181 ggattcagac cgatgaaact ctccgtccat tgctcaatgc gctgcatctc caggctgacg
     3241 atagaccgga acgggcgatt cagaactttg agatactcca cggccgcttc gctgtcgttc
     3301 atcgcaggcc cgccgacaaa gctgaaaccc gtaagcgaga caagctgggc tacgcggggt
     3361 gtattggttt gagcatccac cagaaattcc tggcaggctt cgcggttgtc catgaacgtg
     3421 gaaagcacgg gaacaactgg aagcccctct gattcgagca gccggatcag gtagtcgtaa
     3481 tggcggcagg cgccgcttac aatctgcggc ctggtcagca acagcccgac agcccggttc
     3541 acatccatgc gtgggtggca gtcattttca aacggccgcg cctcgtacca cgcgcgatac
     3601 tcttcgaaag aagagaacaa cgaagccgca tccggatgat aaatcccggt cgagggcctg
     3661 ttttcgggcg cttcgatacg acggatgccc cgggcttgtt ccgggatgta gtgacggatt
     3721 gtatagagca gcagcgaccg gatgttccca ggcgtgggct gaagcagata gcagaaagcc
     3781 gtcaggtagt gtttcacgtc ccgcaggaca cctaccgaag gcgccagccg cagaatggac
     3841 ggcatctgct tgagaagctg ggaatgcttg tgtccgccgg cgctctttcc gtcccggttt
     3901 cgagcgcgtg cgtaactgcc catccaggac cctgcatcgc gtacagcccg gtaaatgacg
     3961 ctccgctctt tttctttcca gagatggaag agtttcatcc cgtccagctt gccgagcctg
     4021 gtgcgcacca tcaggtcgcg catacagttg atggcaatca ccgcgcgatg gcgcgagcgg
     4081 cagcggtcca gggctgcggc gatccgtgcc gcgttgtcat tatcggttac gtggatgatc
     4141 aaaacgatct cagcatcgtc tatgtcgcgc tccaccgccg gccattcggc gtcgggaacc
     4201 ggcagcgtgc aattatgcac ggcaacggcg agcccgagcc ggtactgttc gttgatctcg
     4261 cgctcggcgt tcttgagcgg cgccagcagg ctggatccga catagaagac agtgattttc
     4321 atgatccctg gctcgccctc gatgcttgcc cgttaactcc ttccaggtga tcttccaact
     4381 tgtcgagcgc gttgtgcaga tgatccagaa cgctctgctg cgcggcccag aagcctctgc
     4441 cgtgcgcttc cagcaggcgc tgggcgagac tgcgtgttgc atgaggattc agctctgcca
     4501 ggcgctgccg catcgcctcg tccaccacat acgtgttcgc gatctcgctg tagatccagt
     4561 cgtccacagc accggccgtc gcgctccagc cgaatgtatt gctgacgtgg tgctcgatct
     4621 cggcgactcc gctaaaaccg tgctttagca ttcctccata ccacttcgga tttagaagct
     4681 ttgtacgcac ttccagacgc acggtctcat tgagcgagcg gatcttgacg agcggactca
     4741 ggctgtcaca aacgtacgtt gcggggcgct ctccggtgcg ctgctcgacg gcttgcgtga
     4801 gaccgccgag gtattcgaaa tagtgatcga cgtcggtgat cccgatctcc gcgttatcga
     4861 gattctggta agtgacttcg acccgcgaga gagccttgtc catcagcgtc cgggcagggt
     4921 ctccgtccag cccacggccg taagcgaagc acttacgcgt cacgaagaga tctccaatct
     4981 gttcggtacg atcccagtga ccgccgatga ccatgaaatt gacgttggtg ccgtagttgc
     5041 cgggcgcgtt cgaaaacagc cgggtcgctg cgtcgcacca ggacatgccc tcctgctgcg
     5101 cctgttgaac gtgtttgcga agaaaattca cctcgggcgg ttcatcaagg ctcgcgactt
     5161 tgcgtgccgc tttatccagc aactccatgg tggtgccgaa gagatcgcga aagatgccgg
     5221 acagcacaaa gacaacatcg attcgcggac ggccgagcgt ttccggcgga atgacctcca
     5281 catccgtgac acggttcatg ccgtcgcgct tcggaaccac gcccatcaac aagagcgctt
     5341 gcgcaacgcc ttcgccttgc gtcttgatat tgtccatacc ccagatgacc atcccgatgg
     5401 tttccgggta gcggccacgt tcttcgaggc agcggttgag caagcgcccg atcgtttcct
     5461 gggatttttg gaacgccact cgcgacggca cggcatatgg gttgacagcg tgcgtgttcc
     5521 ggcccgtggg aaggatcttc ggattctgaa ccaggtcgcc gcccggacct ggttcgatgt
     5581 attcgccgcg cagagcgcgc atcaactcgt ccgtttcgtg gttaataccc aattgccggt
     5641 ccaggtcgct taaaaatgta agcatccggg tggagtcttc ggttgggatg gaaacctcac
     5701 gcgccagcag ggaagcagcc tcttcgattc caccaccttc cgccagaagc ttttctatcg
     5761 cgctgcgaag gattgcatcc actcgttcac gctgcaagag gcgttcgccg gacgttgcgc
     5821 tctcctccat caggaccggg tattcgggaa aaccgagact cgccgcgatc tggccgggca
     5881 acgagcggaa ccccatctcc ggccggtcga acgacgcgac cattcggagc atgtctcccc
     5941 gttcggtttg cgcgccaggc ttgccgaata cgtgcattcc ggtgggaatc agccgctgct
     6001 cgatttcgag caactgctca tagagacgta ggacgatccg gtcccgctct
//
LOCUS       DASTMK010000151        39885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_8601, whole genome shotgun sequence.
ACCESSION   DASTMK010000151 DASTMK010000000
VERSION     DASTMK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 39885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39885
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8601"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VFQ79_06410"
     CDS             complement(<1..1298)
                     /locus_tag="VFQ79_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139322.1"
                     /translation="MAPGTEQAPRGRPRFETYLFFFTTFFLILLFVHAPFLSMPFFWD
                     EAGQFIPASLDLYRDGALIPFSTMPNVHPPGLMAVLAVVWTLAGYSITVTRVTMLALA
                     AAAVLVSFLLAIRLSRGLDGAPAFAAVWFLLASPLFYTQSMMAQLDMPAMLFTGLALL
                     FFLHDRFRLAALACTLLVLTKETGVVVPLVLGAWLLREGRRSAAALFLAPLLALAAWL
                     SVLALATGSALGNQDFERYNLVYPLHPVRLTLALLRRFHYLFFAEFRWIGWIAVAWAW
                     KRTDIFRTRAWRVAAAVACVQVLIVTVLGGAVLERYLLPALPVCYAAMAAAGAAVSPR
                     WRIVGQGALILGLVAANFWTSPVWPGPHENNLAMTRLIDVHRQTAAFLEMHLRSGARV
                     ATAWPLSDALRRPELGYVSRPLNVVALEDFERDSIGSLRG"
     gene            complement(1308..1607)
                     /locus_tag="VFQ79_06415"
     CDS             complement(1308..1607)
                     /locus_tag="VFQ79_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEU0139323.1"
                     /translation="MNLTQQPQPNIQLAKRTDYRESYSNSVQVRVNLWDFFLMFGIVE
                     QTAPDQVSIENFQGIYLSPQQAKALMNVLQQNVSQYESTFGEIKLEPQSPDSTIQ"
     gene            complement(1806..2261)
                     /gene="rplQ"
                     /locus_tag="VFQ79_06420"
     CDS             complement(1806..2261)
                     /gene="rplQ"
                     /locus_tag="VFQ79_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU0139324.1"
                     /translation="MRHKRAGYKLKRDVGARRSLLRGLVTSVIEQERIITTVPKAKAA
                     KPLVEKMITLAKRDSLHSRRQAASFLQTPASVKKLFEKLGTRFGQRNGGYTRIVRLGW
                     RKGDGAEQAMLELVGSELVKRAAERAKRREERLRAVKEGREEEDGEEKS"
     gene            complement(2269..3348)
                     /locus_tag="VFQ79_06425"
     CDS             complement(2269..3348)
                     /locus_tag="VFQ79_06425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU0139325.1"
                     /translation="MFKGFQKPKRLVANTETLTERYGMFWAQPFERGFGTTIGTGLRR
                     VLLSSIEGAAITAVRIDGVAHEFSPIPGVVEDATDIILNLKQVPFKMMTDVVKTVRLR
                     VDQPGEVMSGQIETDADVEVLDRNMHIATIGEGGRLNIEMRLKIGRGYVSADRNFDED
                     LPLGYIPIDSVHSPVRKVNFSVEAARLGQMTDYDKLTLEVWTNGAISPQDSIGQAAKL
                     LKDHMAIFINFEELPEVAEEPAERAMNQMNEILNRSVEELELSVRSYNCLKNANIQSI
                     GDLVQKSEAEMLRTKNFGRKSLNEIKEILGGLGLSFGMKFDAQGRLVSPSGQPAPLSP
                     GADFGDTGAPGDEDGPDEDVEHINE"
     gene            complement(3363..3995)
                     /gene="rpsD"
                     /locus_tag="VFQ79_06430"
     CDS             complement(3363..3995)
                     /gene="rpsD"
                     /locus_tag="VFQ79_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU0139326.1"
                     /translation="MARYNGPVCRLCRREGMKLFLKGERCHTEKCAIEKRNFFPGQHG
                     KSRRPKIVGYGLQLREKQKTRRYYGLLEGQFRNLFEKAAKKKGITGETMLDMLERRLD
                     NVIYRMGFGTSRAQSRQIVRHGHIQVNGRKVNIPSFQVKPNDVVEVREKSRNNPAILG
                     ARDATAHAPTPNWLEVDREALRARVLSIPKRDELVQIQLNEQLIVELYSK"
     gene            complement(4080..4487)
                     /gene="rpsK"
                     /locus_tag="VFQ79_06435"
     CDS             complement(4080..4487)
                     /gene="rpsK"
                     /locus_tag="VFQ79_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU0139327.1"
                     /translation="MAKAPAKAGKKKSFKKKEKKVVPMGVVHIQATFNNTIITFTDPL
                     GNVLAWSSSGSLGFRGSRKGTPFAAQQASLTAANKVKEYGLRVAEVRVSGPGAGRESA
                     VRALATVGIEVRQIKDTTPIPHNGCRPPKKRRV"
     gene            complement(4503..4889)
                     /gene="rpsM"
                     /locus_tag="VFQ79_06440"
     CDS             complement(4503..4889)
                     /gene="rpsM"
                     /locus_tag="VFQ79_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU0139328.1"
                     /translation="MARIAGVDLPNRKRAEIGLTYIYGVGRTRSKSVLHRAGVDANKK
                     VVDLSEDEVNRIRQILETEGAVEGDLRKELSMNVKRLIEMGAYRGLRHRRGLPVRGQR
                     THTNARTRKGPRRGTVANKKKASAKT"
     gene            complement(4901..5014)
                     /gene="rpmJ"
                     /locus_tag="VFQ79_06445"
     CDS             complement(4901..5014)
                     /gene="rpmJ"
                     /locus_tag="VFQ79_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU0139329.1"
                     /translation="MKVRASVKKMCDKCKLIRREGVVRVICVNPKHKQRQG"
     gene            complement(5066..5299)
                     /locus_tag="VFQ79_06450"
     CDS             complement(5066..5299)
                     /locus_tag="VFQ79_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU0139330.1"
                     /translation="MDEGAERENRSREAVVIELLPSATYRVELSDRRQMLAHLASAQV
                     RNFVRLRVGDRVQVEPSLEDETRGRIIELLKKR"
     gene            complement(5309..6079)
                     /gene="map"
                     /locus_tag="VFQ79_06455"
     CDS             complement(5309..6079)
                     /gene="map"
                     /locus_tag="VFQ79_06455"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU0139331.1"
                     /translation="MIIRKSPAELEKMRRAGLLVWEILQKLSGVVAEGVTTRDLEAIA
                     EKMMSEAGARPAFKGYYVPAAGSKYPYVLCTSVNDEIVHGMPSTKRVLKKGDVVSIDT
                     GVQLDGYFGDSAITVPVGDIPDETKKLLTVTRESLELAIGKMRVGNRLFDICGTVEKH
                     VTSHGFSIVREYVGHGIGTRLHEEPQVPNYVDRRNENPRLKEGMVLAIEPMVNAGGAE
                     SRLASDKWTAVTKDGSYSAHFEHCVAVTSNGPWVLTRP"
     gene            complement(6076..6750)
                     /locus_tag="VFQ79_06460"
     CDS             complement(6076..6750)
                     /locus_tag="VFQ79_06460"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEU0139332.1"
                     /translation="MPKIGIRTVAVLLFGPPGCGKGTVGKHLTGCFDIPHISTGDILR
                     ENVQAGTELGRQVQSLMENGGLVPDGMVNRLVQDRISRDDCLDGFILDGYPRTLEQGK
                     ALAGMLEEQGLEPLVIHLKVDYNRIVSRLTARRSCPQCGAVYNVISKPPKSDGVCDGD
                     GAGLILRGDDCETVIRQRIEAYEAQTLPLVEYFRQLASKFYTVDGNDGSPTDIAERAC
                     HHIRGE"
     gene            complement(6817..8214)
                     /gene="secY"
                     /locus_tag="VFQ79_06465"
     CDS             complement(6817..8214)
                     /gene="secY"
                     /locus_tag="VFQ79_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU0139333.1"
                     /translation="MKFFEAFANVFRVPDLRNRVLFTLAMLAIYRLGGHIPTPGIDAA
                     RLQEAFQQNKGTLLGFIDLFSGGMFSQMAIFALGIMPYITASIILQLMTVVVPTLEKL
                     QKEGELGRRKITQWTRYLTIILALLQSLGIATGLQSMEGNFVLNPGIGFVLMTMLSLT
                     TGTAFIMWLGEQITERGIGNGMSLIIFAGIVVGLPHAITEIYNNVFVTRNWSALHLII
                     ILLVMVAVVAFIVLVERGERRIPVQYAKRVVGRRMMGGQSTHLPLKVNAGGVIPVIFA
                     SSLLAFPQTLLQVPAFRDNRWLRGMLESLGYAEPLHALLFVLGIVFFCFFYVSIIFNP
                     NEAADNMRKYGGFVPGIRPGKNTAEYMNKILTKITMVGGLYLALLSLIPQIMISGIKL
                     QHLPLAGNFIDAYFPRWLLDGLGVNFYFGGTSLLIVVGVAMDTVNQVEAQLIMRHYEG
                     FTPRAGRIRGRRSTF"
     gene            complement(8234..8683)
                     /gene="rplO"
                     /locus_tag="VFQ79_06470"
     CDS             complement(8234..8683)
                     /gene="rplO"
                     /locus_tag="VFQ79_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU0139334.1"
                     /translation="MNLSGLKPPAGQKHSRRRIGRGMGSGRGKTSGRGQKGQHSVSGF
                     NQMRGFEGGQMPLHRRMPKRGFTNIFKKEYAIVNVGHLGELEGDSFDPARLVELGVVK
                     KLGDGLKILGDGELKRAIRVQAHLFSKAAAEKIQAAGGSVEVVGRTK"
     gene            complement(8692..8874)
                     /gene="rpmD"
                     /locus_tag="VFQ79_06475"
     CDS             complement(8692..8874)
                     /gene="rpmD"
                     /locus_tag="VFQ79_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU0139335.1"
                     /translation="MSEGKIKIKLVRSPICTPEKHKKIVRSLGLKKINQVVERPDSPS
                     FRGMVAKVPHLLAVVE"
     gene            complement(8878..9405)
                     /gene="rpsE"
                     /locus_tag="VFQ79_06480"
     CDS             complement(8878..9405)
                     /gene="rpsE"
                     /locus_tag="VFQ79_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU0139336.1"
                     /translation="MKKVDPQLFNLKEQVVSINRVTKVVKGGKNLSFSALVVVGDAGA
                     KVVGFGTGKAKEVPSAIKKGIEAAKKNLRKINVLETTISHQVVGRFGSGLVLLKPAPE
                     GTGVIAGGPVRAVIQAVGIPNVLTKSIGSRNPHNVVKATIEGLEQLRDRTAMADLRGV
                     AVEKLDTRRTGPSGN"
     gene            complement(9423..9782)
                     /gene="rplR"
                     /locus_tag="VFQ79_06485"
     CDS             complement(9423..9782)
                     /gene="rplR"
                     /locus_tag="VFQ79_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU0139337.1"
                     /translation="MIRKVSKNNVRIRIHRRIRKGIRGTPERPRLVVFRSLKHIYAQV
                     IDDREGRTLAAASSGEKSAAGNGGNVSGAKEVGKLLAERAREKGVTKVVFDRGGYIYH
                     GRVKALADAAREAGLEF"
     gene            complement(9815..10351)
                     /gene="rplF"
                     /locus_tag="VFQ79_06490"
     CDS             complement(9815..10351)
                     /gene="rplF"
                     /locus_tag="VFQ79_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU0139338.1"
                     /translation="MSRVGKKPIPLPKGVKITAAGELQVEGPKGKLSVPIPPGVVIRQ
                     DDGNVEIARDGDEYAALHGLTRALAANAIQGVSGGFTRELDIVGIGYRCDVKGKIATF
                     ALGYSHPIEFLLPDGVDLKVDKQTHLTLSGIDKQLLGQVASDIRALRPPEPYKNKGIR
                     YTGEVLRKKVGKTGAGSK"
     gene            complement(10449..10844)
                     /gene="rpsH"
                     /locus_tag="VFQ79_06495"
     CDS             complement(10449..10844)
                     /gene="rpsH"
                     /locus_tag="VFQ79_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU0139339.1"
                     /translation="MVSDPIADMLTRIRNAQIARHPKVDVPGSKLKVEIARILKEEGY
                     ILNYKLADEGIRGVIRVYLKYTPGNVPVMSHLERVSRPGCRVYAGSKEIPRVLGGMGI
                     NILTTPRGVMTGSTARKEGVGGEILCQVW"
     gene            complement(10866..11075)
                     /locus_tag="VFQ79_06500"
     CDS             complement(10866..11075)
                     /locus_tag="VFQ79_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU0139340.1"
                     /translation="MATTAKIARDLRLEKAKQARTKKLRCRHRNRCRRCGRPRGYLRK
                     FNLCRICFRGLALAGEIPGVIKASW"
     gene            complement(11097..11639)
                     /gene="rplE"
                     /locus_tag="VFQ79_06505"
     CDS             complement(11097..11639)
                     /gene="rplE"
                     /locus_tag="VFQ79_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU0139341.1"
                     /translation="MGARLREHYTKNVIPGLVKEFGYKNVMAVPKLEKISLNIGLGEA
                     TQNSKLMDPAVAELAQIAGQRPVVTKASKSIAAFKLREGMSVGCMVTLRGDRMYEFFD
                     RLVNVSLPRVRDFRGVSTKSFDGRGNYTLGIKDQLIFPEIDYNKVEKIKGMNICITTT
                     ARTDAEGLALLRQLGMPFRQ"
     gene            complement(11730..12125)
                     /gene="rplX"
                     /locus_tag="VFQ79_06510"
     CDS             complement(11730..12125)
                     /gene="rplX"
                     /locus_tag="VFQ79_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU0139342.1"
                     /translation="MARQQQKATPPRHQGVPKTRIKSKIKKDDIVKVITGRDKGKTGR
                     VLNVDREKGRALVEGVMLIKRHTRPNPSKQIKGGIAERESTIHLSNLMVVTSGGVATR
                     IGYRVEGTGAGQRSVRIARKTGEVLDKKS"
     gene            complement(12125..12493)
                     /gene="rplN"
                     /locus_tag="VFQ79_06515"
     CDS             complement(12125..12493)
                     /gene="rplN"
                     /locus_tag="VFQ79_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU0139343.1"
                     /translation="MIGMRTILDVADNSGAKRLSCILPRGGDLGLRAGLGDIITASVK
                     EAAPDSNIKKGKVVRCVIVRMRKETRRRDGTYIRFDSNAAVLITETGEPVGTRVFGPV
                     ARELRDKRFMKIVSLAPEVI"
     gene            complement(12490..12798)
                     /gene="rpsQ"
                     /locus_tag="VFQ79_06520"
     CDS             complement(12490..12798)
                     /gene="rpsQ"
                     /locus_tag="VFQ79_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU0139344.1"
                     /translation="MTEAPVAGIRNEKTGQVVSTKMQKTIVVEVTRRVPHPLYKRIVT
                     RRKKFYAHDEEQTARPGDIVRIIESRPLSKLKRWNLAEVLRRAAQVGTLEEEEKETSL
                     "
     gene            complement(12801..13028)
                     /gene="rpmC"
                     /locus_tag="VFQ79_06525"
     CDS             complement(12801..13028)
                     /gene="rpmC"
                     /locus_tag="VFQ79_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU0139345.1"
                     /translation="MRAEKVRELDSKELNIKLREMEDQLFRLKFQIGMGQADGLKKLR
                     QIRKDRARMLTILHERALAGQGRDRRRKGAR"
     gene            complement(13032..13448)
                     /gene="rplP"
                     /locus_tag="VFQ79_06530"
     CDS             complement(13032..13448)
                     /gene="rplP"
                     /locus_tag="VFQ79_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU0139346.1"
                     /translation="MLMPKKVKHRKQQRGRMRGKAWRGSDLSFGDFGLKVMECGWITD
                     RQIEAARIAMTRFIKRGGKVWIRLFPDKPITKKPAETRMGKGKGAPEQWVAVVRPGKV
                     LFEMEGVPRDVAEEAMRLAAQKLPLPCKMVARERVE"
     gene            complement(13802..14437)
                     /gene="rpsC"
                     /locus_tag="VFQ79_06535"
                     /pseudo
     CDS             complement(13802..14437)
                     /gene="rpsC"
                     /locus_tag="VFQ79_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(14441..14788)
                     /gene="rplV"
                     /locus_tag="VFQ79_06540"
     CDS             complement(14441..14788)
                     /gene="rplV"
                     /locus_tag="VFQ79_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU0139347.1"
                     /translation="MEARAEARYIRVSPQKARLVVDQIRGRKAGDAITILRSTNKGIA
                     PAVEKVLRSAISNAENRFNDVDVDALLVTQAYVNEGPRMKRIRPAPMGRAFRYQRRSA
                     HIVVAVGDRKTEE"
     gene            complement(14801..15085)
                     /gene="rpsS"
                     /locus_tag="VFQ79_06545"
     CDS             complement(14801..15085)
                     /gene="rpsS"
                     /locus_tag="VFQ79_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU0139348.1"
                     /translation="MGRSLSKGPFVDSHLMVKVEAMNSKNEKKVLRTWSRRSTILPEF
                     IGHTIAVHNGKKFIPVYITENMVGHKLGEFSPTRTYKGHAAKTEKSSRPS"
     gene            complement(15099..15929)
                     /gene="rplB"
                     /locus_tag="VFQ79_06550"
     CDS             complement(15099..15929)
                     /gene="rplB"
                     /locus_tag="VFQ79_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU0139349.1"
                     /translation="MAIKTYRPTTPTRRFQTVVSRNEITKDKPEKSLVTGKKRSGGRN
                     ALGRITSRFIGGGAKKAYRIIDFKRDKAGVPARVAAIEYDPNRSARIALLNYADGEKR
                     YILAPVGLQVGGKVVSGPEADILVGNALPLQQIPAGTFVHNVELRPGKGGQMVRSAGS
                     QAQLVSKEGGYALLKLPSGEVRRVQLECMATVGQVGNLDHENVSLGKAGRKRWMGKRP
                     HNRGVTMNPVDHPHGGGEGRTSGGRNPVTPWGQPTRGFKTRNNKRTDKWVVTHRSKKR
                     "
     gene            complement(15940..16227)
                     /locus_tag="VFQ79_06555"
     CDS             complement(15940..16227)
                     /locus_tag="VFQ79_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU0139350.1"
                     /translation="MNRFDVIRRPIITEKGVEKKDNERTLCFEVAVDANKTQIRQAVQ
                     QLFKVKVEEVRTATQEGKLRRRGRFTGYRPDWKKAYVRLKPGEKMPEYAEI"
     gene            complement(16224..16850)
                     /gene="rplD"
                     /locus_tag="VFQ79_06560"
     CDS             complement(16224..16850)
                     /gene="rplD"
                     /locus_tag="VFQ79_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU0139351.1"
                     /translation="MPSIDVFDLNNQKVGELELADSVFGAAVNEALMYEAVRHYMASR
                     RSGTVKTKTRWEVSGSGKKLWRQKGTGRARVGSIRSPLWRHGGTAHGPQPRDYSYKLP
                     RKMLLGALRSALSAKLRDGELKVIQAFTLPDHKTRSMRSALGKIEAGRKVLLVENEEN
                     RNLQLGCRNLPGVTLVASREVTVYDLLGHTQVVLSESAARKLSEALAL"
     gene            complement(16856..17488)
                     /gene="rplC"
                     /locus_tag="VFQ79_06565"
     CDS             complement(16856..17488)
                     /gene="rplC"
                     /locus_tag="VFQ79_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU0139352.1"
                     /translation="MSPGILGKKIGMTQVFRPDGQVVPVTLLKAGPCVVVQRKTPVVD
                     GYDAVQLGLMEFVKASRLTKPAAGHLKKANVEGVKFTKELRIRPGDDDLKPGDRILVD
                     QFAPAQKVDVTGISKGRGFAGFVKRHHFRGGEGSHGSMFHRAPGSIGASSYPSRVFPG
                     MKGAGHMGTQQVTVRNLEIVGIDAEDNVLMVKGAVPGPNGGYVVVRRAKR"
     gene            complement(17560..17883)
                     /gene="rpsJ"
                     /locus_tag="VFQ79_06570"
     CDS             complement(17560..17883)
                     /gene="rpsJ"
                     /locus_tag="VFQ79_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU0139353.1"
                     /translation="MLRERIRIRLKAYDHRVLDQSTTEIVETAKRTGAQIAGPIPLPT
                     SKNRFTVLRSPHVDKKSREQFEIRTHKRLLDILEPTQDTVDALMKLDLPAGVDVEIKA
                     FGKGK"
     gene            complement(17982..19169)
                     /gene="tuf"
                     /locus_tag="VFQ79_06575"
     CDS             complement(17982..19169)
                     /gene="tuf"
                     /locus_tag="VFQ79_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU0139354.1"
                     /translation="MAKEKFERSKPHINVGTIGHIDHGKTTLTAAITKCLAKHNPKVK
                     FRSFDSIDNAPEEKARGITIAVSHVEYETPNRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPYVVVALNKVDAVDDAELLELVELEVRELLK
                     SYQFPGDEVPVVRVSALEALNGDPEWEKKIDELMEAVDNYIPVPERAIDKPFIMPIED
                     IFSIQGRGTVVTGRIERGIVKVGEEMEIVGFRDTRKTVVTGVEMFKKLLDEGRAGDNV
                     GLLLRGIDKDDVERGQVIAKPGSITPHKKFRGEVYVLSKEEGGRHTPFFKGYRPQFYF
                     RTTDVTGVAQLPEGTEMVMPGDNVNLEVELITPVAMDKGLRFAIREGGRTVGAGTVTE
                     ILE"
     gene            complement(19209..21302)
                     /gene="fusA"
                     /locus_tag="VFQ79_06580"
     CDS             complement(19209..21302)
                     /gene="fusA"
                     /locus_tag="VFQ79_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0139355.1"
                     /translation="MPRTVDIEKMRNIGIMAHIDAGKTTTTERILYYTGRTYKIGEVH
                     EGTAVMDWMEQEQERGITITSAATTCQWRDTQINIIDTPGHVDFTAEVERSLRVLDGA
                     VAVFDAVAGVQPQSETVWRQADKYQVPRICFINKMDRIGADFYRAVDTIVQRLKCRPV
                     PIQIPIGSEDHFKGVIDLVEMRARIWHEETLGAKFDDQGIPEDLAAKAQEYREQLVEA
                     VSESDEALFEKFVNGQPLTNEELRTGIRRATIALKIFPVICGSSFKNKGVQNLLDAVV
                     DYLPSPTEVPPVQGTAIDNPDEIMVRESSDAEPFAGLIFKIMTDPFVGQLAFLRVYSG
                     RVVSGESVYNVAKGRRERVGRLVKMHANKREEVQDVYAGDIVAAVGLKTVVTGDTVCD
                     EKMPILLESIDFPTPVIQLAIEPKTKADQEKLGMAIAKLVTEDPTLKVSTDPDTGQTI
                     LAGMGELHLEIIVDRMQREFGVGANVGKPQVAYRETIRKPSQAEGRFVRQSGGKGQYG
                     HVKIRVEPLPAGTGFEFENAIVGGSIPKEYIPAAEKGMVEALEGGVLAGYPMSDLKAV
                     LYDGSYHDVDSSEMAFKIAGSMAIKAAATAAKPVLLEPIMAVEVVVPEEYTGDVYGDL
                     NSRRGRIEGIELRGTSQIIKSMVPLSEMFGYATELRSRTQGRGSFTMHFGRYDEVPAA
                     ISEEIVSRVQGKVTR"
     gene            complement(21334..21804)
                     /gene="rpsG"
                     /locus_tag="VFQ79_06585"
     CDS             complement(21334..21804)
                     /gene="rpsG"
                     /locus_tag="VFQ79_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU0139356.1"
                     /translation="MPRRRRPELKEIAPDPVYNSTLVEKFVNSMMWQGKKTVAQGVFY
                     TAMDTIRERTGDDPLKLFKKAVENCKPLLEVKTRRVGGANYQVPIEVPQNRRTSLAIR
                     WLILNARGRAEKGMPEKLANELNDAANMRGGAIKKKDDVHRMAEANKAFAHYRW"
     gene            complement(21814..22188)
                     /gene="rpsL"
                     /locus_tag="VFQ79_06590"
     CDS             complement(21814..22188)
                     /gene="rpsL"
                     /locus_tag="VFQ79_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU0139357.1"
                     /translation="MPTFNQLVRKGRKLQRYKTASPALQQCPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVVRGTL
                     DAAGVANRKQGRSKYGAKRPKS"
     gene            complement(22563..23951)
                     /locus_tag="VFQ79_06595"
     CDS             complement(22563..23951)
                     /locus_tag="VFQ79_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139358.1"
                     /translation="MFETRSEWRDSPVADWSPLLASAAVAAALALAVNHIFRSPVESF
                     PKAILRPTFAVLSVFLASLCAALLAYRFTRINSNAPRLNVALAFAGWSAWMAPLVLIL
                     MTWNGAVWLALLSGALAIGFGRLVQRYRRLLASGMETGIDLPSTPVFAEIFASLAERP
                     PPWSRCALGLSLCIQSGIAALMFGNKILGALLFAAAGLFLAWSLRKVEDNFTPIRFRP
                     SRSVVSVLCAIALMAWIGGFGRRPLYPGSRTGGSQGKAAVRADSGLLSSVILKSKNPR
                     HVVLIAPRLERPPSATPALARKSTRIPFSGEYWFLSWLSSRPSATPMIQFGSPMEWLF
                     TNMDRHPLTMIARQPLGTQLSMSCCNSVEVEVSALPQEASTVSVDMYLVRTGEEGRFR
                     RQSLGTQELSELQPPEKGLAAGRLQGTLHFAMPASSVIDDFDEIHAQFFLHMPRSTRS
                     ARVEVLGFTLRQ"
     gene            complement(24072..24665)
                     /locus_tag="VFQ79_06600"
     CDS             complement(24072..24665)
                     /locus_tag="VFQ79_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU0139359.1"
                     /translation="MPDLTEIRVAGFGGQGVILAAMIIGKAASIFEDGYATLTQNFGP
                     EARGSACSAQVILSNEPVLYPYITQPDLLIAMSQDAFNKFVPELKDGGLLLVEEDLVR
                     VDNLRAGIRVYSVPATRLAEQLGKRLVLNIVMVGFFAAVSGVLNPDALRKAVADSVPP
                     SFKDLNLKAFDKGYEYGLTQSPVSPEESESPVGMEVE"
     gene            complement(24653..25531)
                     /locus_tag="VFQ79_06605"
     CDS             complement(24653..25531)
                     /locus_tag="VFQ79_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU0139360.1"
                     /translation="MSEVLEQVEINPVEPFLRSERMPHIWCPGCGIGTTVNCFARALV
                     ESGANLDKVAVVSGIGCTGRVAGYVELDSFHTTHGRAIPFATGLKLANPELQVVVYSG
                     DGDLTAIGGNHLIHAARRNIDIKVICVNNLIYAMTGGQSAPTTPQLAISTTAPYGSYE
                     PTFNLPSLAEAAGASYVARWTTFHVRQLTRSMAEVFNRKGFCFIEVLSPCPTLYQRRN
                     KMGDGLDAMKFYKEKSKVRHGASTSEVDLTFDGEIVVGKFVDRERPDYLDVMKTQMRS
                     KLHEEYVDPLEAPCQI"
     gene            complement(25528..26685)
                     /locus_tag="VFQ79_06610"
     CDS             complement(25528..26685)
                     /locus_tag="VFQ79_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU0139361.1"
                     /translation="MHADPKGVLTGAHFLDGDQACTEGALAAGARFAAGYPITPSTEI
                     VERFASRIPTVGGTFIQMEDELAASITILGAVWGGAKAFTVTSGPGFSLMMEHIGYAV
                     MTETPCVFVNVQRGGPSTGLPTLPAQADMMQARWGSHGDYEIIAICPSSPQECFDLTI
                     EAFNLSEEFRVPVMVMMDECVGHMTEKVVIPPASEIRITPRRRATKPPGQFHLYATNG
                     DAVPEMVHAGEGYKFHITGLTHDERGYPAMNPQMQDKLVRRLKDKIRNASKRLTLVEE
                     EGTEDADVIVVSYGITARVAQRAIELARQQGIKAGKLRLIAAWPFPEQKIKELAARTR
                     VFVVPELNLGQISLEVERAAGGQARTVPVTHAGGAVHRPEDILHAILEALR"
     gene            complement(26675..26923)
                     /locus_tag="VFQ79_06615"
     CDS             complement(26675..26923)
                     /locus_tag="VFQ79_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU0139362.1"
                     /translation="MAKGSVSILVERCKGCGFCVEFCPTKVLSLSSAFNSKGYHPPHM
                     IAPEKCSGCDLCGMYCPDFAIFGYKFRQAAGTGVVHAR"
     gene            complement(26987..27712)
                     /locus_tag="VFQ79_06620"
     CDS             complement(26987..27712)
                     /locus_tag="VFQ79_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139363.1"
                     /translation="MADDIIETRQLTKEFIRDEFHVVALKDVDLSIAKGEFVALMGPS
                     GSGKSTLLHLIAAMDRPTGGQILVLGSDLRALSDREIARWRNMHIGFVFQSFNLIPVL
                     TAMENVELPLKLTNLKKKERLEHAATALKLVGLGDRMAHYPRQLSGGQEQRVAIARAI
                     VTDPDLILADEPTGNLDASSAQEVLTLLSRLNQEYGKTIVMVTHDPHAARSATKIRYL
                     DKGALLPEGRTPEDWTTIATRGH"
     gene            complement(27715..29058)
                     /locus_tag="VFQ79_06625"
     CDS             complement(27715..29058)
                     /locus_tag="VFQ79_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0139364.1"
                     /translation="MPMETELKSLRIDRARKEAPRGSGWSRRWIIAGIALLVIAGAAL
                     YGYSLLNRAAEVEVHRVQAQTTGGNAANTIVLNATGYIVAAHKIQVASKVLGRVAWIG
                     VDKGDKVREGQVIVRLEDDEYRAQLQQAKGQLAALEARLSELLNGSRPEEIERARAEV
                     EEARADLANARITLDRSNKLVGGGVLARQTLDDARAAYEARAARVIALEKAYELVRIG
                     PRQEEIAAARGQVEQARGAVAFGETQLENTVIRAPVTGTILERAVEKGEFVTTSFVGE
                     RGAKGYVVSLADLRDLEVELDISQNDFAKLDPRQRGIVTTDAFPDRKYEGVIDEISPE
                     ANRQKATVQIKVKVLEPDDYLRPEMNASVAFYSEEKAEPGGAPVRTVVIPPSAVRDNA
                     VFVVLDGKAVRRTVKPAGTTPQGVRIAEGLIGGEDLIVNPPADLKDGDRVKERKG"
     gene            complement(29115..30281)
                     /locus_tag="VFQ79_06630"
     CDS             complement(29115..30281)
                     /locus_tag="VFQ79_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139365.1"
                     /translation="MAIPLAYNLRNLAVRKTTTVMTALGIALTVAVLLAIMALVEGLQ
                     TSLGATGNPLHVLVMRKGSTSELTSNFNRSTFQDLIFKPGIARSKNGEPMASLEMVTV
                     ISLESPDNPDGINITLRGLAPIGIEMRDEIRLSAGRWFQPGRREVVVGRSIANRYSAA
                     QIGNRIRFGRGDWEIVGVFDAGRSAANSEIFADLNQVASDYNRSEVLSSALVRAVDPV
                     AVQALVNDLQSDRRFNVMAQTERSYYEQQMQSAAPIEFMGMFVAVIMAIGSSFAAMNT
                     MYAAVARRSAEIGTLRVLGFSKAGILSSFLIESLLLSLVGGLLGCLLVLPLNSIETGI
                     GSFVTFSEIAFNFRVTGELMLKGLLFALLMGAIGGVFPARMAANKEILTALREI"
     gene            complement(30293..31450)
                     /locus_tag="VFQ79_06635"
     CDS             complement(30293..31450)
                     /locus_tag="VFQ79_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0139366.1"
                     /translation="MSRYFPLVVKNVLRNRRRSILTVLSIAASLCLLGVLMAMYHAFY
                     FGEATPEQALRLITRNRISLANVMPVSYRDRMKQVPGVRDITIIQWFGGIYKDNDFKN
                     FFARMAVEPEKLYVLYPEYRIPDDQKEAFRRERSACLVGRPLMERYGWKLGDRVTLIG
                     DIFPVTMEFTIRAVYDAPNDNENFFFHYEYLRESIPEARRDAVSTFAILADSPDSVPQ
                     IAHDIDAMFQNAPAQTKTEPERAFQLSFLGYLGNVKLFLLSICGAITFTLLLVSGNTM
                     AMSVRERVREVGIMKTLGFTSPAVLGIIISEAVFIALIGGVLGLALSEVICAFIRQGP
                     ITMVDLKTLSLPPAVILGGAALACFIGAVSSFLPAWSASRRSIVDSLRFSD"
     gene            complement(31507..31941)
                     /locus_tag="VFQ79_06640"
     CDS             complement(31507..31941)
                     /locus_tag="VFQ79_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0139367.1"
                     /translation="MRQFLRRHHRIAIDTSIFIYEMEANGRYVTLADGIFQWLEQSGH
                     AGVTSTITMTELLVPPYREKDEDRVNLFYGLLTTYPNLEWIPPGLEIADLAARFRAEH
                     RLKTPDALHAATAVWSGATGFVTNDPVFKRVTAFETFVLDDA"
     gene            complement(31947..32168)
                     /locus_tag="VFQ79_06645"
     CDS             complement(31947..32168)
                     /locus_tag="VFQ79_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU0139368.1"
                     /translation="MTEATLSTKNQIVIPREAREALALKAGDKVLIVVRGSTVVMLPK
                     PASHQAALRGLAKRVYSPQHLRKERRSWD"
     gene            complement(32226..32651)
                     /locus_tag="VFQ79_06650"
     CDS             complement(32226..32651)
                     /locus_tag="VFQ79_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HEU0139369.1"
                     /translation="MTAEQLFSILNLVTVAAWLPLVFLPRMRWASRIVPVVMPLLLAA
                     VYVVLMAVVLMRSEGGFSSLAGVRALFDNPWALLAGWTHYLAFDLFIGGWEARDAQRR
                     GIPHLLIVPALVLTFLLGPAGLLLYRTIRWLAPPSEPRA"
     gene            complement(32648..33643)
                     /locus_tag="VFQ79_06655"
     CDS             complement(32648..33643)
                     /locus_tag="VFQ79_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139370.1"
                     /translation="MDLKQRTVRFHPWVRAAGLLDRLWRTSPPLTAVGALMTVVAGFS
                     LLGMLVDPRMITGAPAWLKPFKFAVSTAIYSLTLAWIFTWLPDWPRVRRLVGWTTAIV
                     FVLEVAIIDAQAWRGTTSHFNVSTALNMVLFLVMGAAILIQTFVSVAVAVALWRSHFS
                     DRTLGWALRLGMTLTIAGALTGPLMTRPTAAQLTHARAGGQMTTAGAHSVSGVDGGPG
                     VPVTGWSREHGDLRVPHFIGLHSIQVLALIGAGLRRWRRPEAVRVRALLAAAASYAAL
                     FLLLLWQALRGQSVVAPDATALASMAIWAVTTVLVLGWIGRGSHATSAGALKEAS"
     gene            33770..34837
                     /locus_tag="VFQ79_06660"
     CDS             33770..34837
                     /locus_tag="VFQ79_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0139371.1"
                     /translation="MTRREMIAILPALMVPRGTGAAPAIEYAGLSGPVGAGVGAKRVR
                     TLRIEEPGVYENILVDGEWAEEDLVRIRTDGVVLRNCTIRYGRRDALEVYGRNVRVEN
                     CHIHHVINGTYRAEKNLDAHGITGRPLNLTIRNTEISHVSGDAIQFDPGRRAEPYPWD
                     NVLVDHCFLWTSPLDKDYAGYKQGERPGENAFDSKTHAGEPRKSRITFRNCLLKGWGH
                     GAIGNGAALNLKEKVQAVVDNCVLVENDIGFRCRGTLGSAWVTARNCTVYRTSRVFRL
                     EHEVRNIKIFHLALGEGIGQLYDNAPGPGEGFEEHGSRKAPPLAQWPYNRLPVGEVIA
                     PGTPPSSADDAVPTALATGWS"
     gene            complement(34839..36116)
                     /locus_tag="VFQ79_06665"
     CDS             complement(34839..36116)
                     /locus_tag="VFQ79_06665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0139372.1"
                     /translation="MKKGISLSLITVLLAGAMFAQTAASSAALSGSAGTVLAQERLAR
                     VGQLLQRYTDEKRIAGAVALVLRDGQPVYERAVGWADREAGRRMTVDTIFRIASQTKA
                     ITSAAILALVEEGKIGLNEPVGNFIPTFKKTTVAVKDGSGMKTEPARRAITIQQLLTH
                     TAGISYGRESHIEALYAQKGLGPAAGNGWYTADKDEPICITMERLGTLPFAAQPGESF
                     VYGYNTDILGCVVEKASGMPLDQFIQTRITGPLGMKDTRFFLPAAERDRLAAVYASGS
                     DGLIVRAPEGPRGQGSYVDGPRKSFAGGAGLTSTARDYARFLEMIRNGGKLDGVRILA
                     PRTVALMTTNQSGTLPSGDGLGFGLGFQTIDRYGAKGMAGVGAFGWGGAYGTTYEVDP
                     ASRMVLVLMLQLMPNATNIGEKFPLTVYQSLVD"
     gene            36244..37533
                     /locus_tag="VFQ79_06670"
     CDS             36244..37533
                     /locus_tag="VFQ79_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0139373.1"
                     /translation="MVLIIADDLGWGDIAINGAPDIRTPNIDGIARGGVRFTQAYANA
                     PECTPTRCALMTGRYQQRAGGLECAIGVNNIGRYDEAEWLQKRGELGLPASEQTISKM
                     FQAAGYDTALTGKWHLGYLEKHWPREHGFQYSFGILGGNADYYTHEEQGEGAGKTQMF
                     ENGSKVRRQGYMTDLIADAAIGWLKQRTSKPFFLYLPFTAPHSPIQAPEDFDPRTGTA
                     PHRPKHRPTYAKMVERLDTRIGDVLRQLDAMGVARNTIVVFASDNGGDANGRNDPFKG
                     RKSSVFEGGIRAPLHIRWPGVIPAGRAIPQVAMTMDLAPTLLGAVGAGGSARFDGIDL
                     MPFLNGTQTPVRRTVFWRYKRGTNIRKAVRHGRLKYVDDNGVKALYDLRVDSKETTDL
                     LKSRPDAVKDLEDRLAAWEKDVESPRLKEFRKGHTQC"
     gene            37527..39119
                     /locus_tag="VFQ79_06675"
     CDS             37527..39119
                     /locus_tag="VFQ79_06675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0139374.1"
                     /translation="MLKGTWMKNITSVAIALFVSGAAAFGAGDLPLPRFADPARRAKL
                     EKALPELEKVFEEYRLRRGIPGLVYGVVIDGDLVSVKTMGVRDRESNAPVTPDTVFRI
                     ASMTKSFTALAILKLRDEGKLSLDDPVSKWIPQFARLEYPTRDTAPLRVRQLLTHSAG
                     FPEDNPWGDQQLGVSEDQLTAWLEKGLPFSTPPGTAYEYSNYGFALLGRIVSNASGMP
                     YRDYLEEQVLAPLGMTHSTLEPSSVPADVRATGYRKTGETYSVEKPLAHGGFGSMGGL
                     LTSARDLGRYVAFQLSAFPPRDEEDGGPVKRSSRREMQQVWRQGTFNVRRSAGDSELQ
                     VEAGGYGYGLAVWRNCRFSHVVGHGGGLPGFGSYMLWLPEHGVGMFAMANLTYAGPRQ
                     PIEESLNILRKTGALKPRELPASPVLLSTRDAIVRLWHSWSEKGAEQMAADNLFLDRP
                     AAERRAEIERIKGAVGACRPPEKIDAENWLRGTFRMNCERGAVDVFFTLAPTKPPRVQ
                     HLSFARTSAGVARSTQTSACSE"
     gene            39345..>39885
                     /locus_tag="VFQ79_06680"
     CDS             39345..>39885
                     /locus_tag="VFQ79_06680"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU0139375.1"
                     /translation="MQASSTPQAVAIRSRIVLAAASGLSNQQIALKLDIPEVTVGKWR
                     RSFADQGLEGLRDAARSGRPPKHDASVWQRVQTAACQQPESQGRWTVRTLAREVGLPH
                     STVHAILNASELQLHRIRTFTFSPDPDFEAKLLDIVGLYLNPPENALVLCVDEKPGIQ
                     ALDRTQPLLPLRAKQPRSWT"
BASE COUNT         7634 a        12899 c        11242 g         8110 t
ORIGIN      
        1 ttgccgcgca gactcccgat ggaatcccgc tcgaaatctt ccagcgctac gacgttgagc
       61 ggtcttgaaa catagccgag ctccggacgc cgcagagcgt cgctgagcgg ccatgccgtc
      121 gccacgcgcg ctccggaacg aagatgcatt tcgagaaaag cggcggtctg gcggtgcaca
      181 tcgatcaggc gcgtcatcgc caggttgttc tcgtgcggcc ccggccatac gggggaagtc
      241 cagaaattgg cggccaccag gccgaggatc aatgcgccct gccccacgat tctccatcgc
      301 ggggaaacgg cggcgccggc ggccgccatg gcagcgtagc aaaccggtag cgcgggcagc
      361 aggtagcgct ccaacaccgc gcctcccaaa accgttacga tgagcacctg aacacacgcc
      421 accgccgcgg ccacccgcca ggcgcgtgta cggaagatat ccgttcgctt ccaggcccat
      481 gccacggcga tccagccgat ccaccggaac tccgcaaaga agagatagtg gaaccgccgg
      541 agcaaggcca gcgtgagccg gaccgggtgc agcgggtaga cgaggttata ccgctcgaag
      601 tcctggttcc ccagcgcact gcccgtcgcc aacgccagca ccgacaacca ggccgccagg
      661 gccagcagcg gcgccaggaa cagggcggcg gcgctccggc ggccctctct caggagccat
      721 gcccccaaca caagcggcac caccacgccc gtctctttcg tcagtacgag tagcgtacag
      781 gcaagcgccg ccagccgaaa ccggtcgtgc agaaaaaaca gcagcgccag cccggtgaag
      841 agcatggccg gcatgtcgag ctgcgccatc atcgactggg tatagaacaa aggcgaggcc
      901 agcaggaacc atacggcggc gaaagccgga gccccgtcga ggcccctcga tagccggatg
      961 gccagcagga aagacaccag cacggccgcg gcggcgaggg ccagcatcgt gacgcgggta
     1021 acggtaatgg agtaccccgc cagagtccag acgaccgcca acacggccat caaccccggc
     1081 ggatgcacgt tcggcatcgt gctgaaggga atcagcgccc catcccggta caggtcgagc
     1141 gaggcaggga tgaactggcc ggcttcgtcc cagaaaaacg gcatgctcag gaagggagca
     1201 tgcacaaaca gcaaaatcaa aaagaaggtc gtgaaaaaga acaggtatgt ttcaaagcgg
     1261 ggccgcccgc ggggcgcttg ctcggtgccg ggcgccatcg ccaatttcta ttgaatcgtg
     1321 gaatccgggc tctgcggctc cagcttgatt tcgccgaagg tcgattcata ttgcgacaca
     1381 ttctgctgaa gcacgttcat cagagccttc gcctgttgcg ggctcagata aataccctgg
     1441 aagttttcga tggaaacctg gtccggggcg gtttgttcga cgatgccgaa catgagaaag
     1501 aaatcccaca ggttcacacg cacctgcacg ctgttggaat aactttcacg ataatccgtc
     1561 ctcttcgcca gttggatatt gggctggggc tgttgcgtaa gattcatata aaaaattcgt
     1621 ctcccgttcc gcaagcagca gccacccggc ggcgctggaa cgcataaaca gcatacacaa
     1681 cccggcacac ggcgtcactg gaaagaagct tgtttggaga gcttcacggc agggtgtagt
     1741 accagtgggg gtacagggga gggaggaacg ggcgactcca ccaaaaggat gccgcccgtt
     1801 cgaaactagg acttctcttc gccgtcttcc tcttcgcggc cttccttcac ggcacgcaac
     1861 cgctcttcac ggcgtttggc gcgctccgcg gcgcgtttca ccagttcgga accgaccagt
     1921 tcgagcatcg cctgttcggc gccatcgccc ttccgccagc cgaggcgaac gatgcgggtg
     1981 taaccgccgt tccgctggcc aaatcgggtt cccagctttt caaacagctt cttgacggaa
     2041 gccggggtct ggagaaagga ggcggcctgc cggcgcgaat gcaggctgtc tctcttggca
     2101 agcgtgatca tcttttccac cagcggcttc gcggcttttg ccttcggcac ggtggtgatg
     2161 atgcgctcct gctcaatgac gctggtgacc agccctctca acagcgacct gcgagccccg
     2221 acgtcccgct tcagtttgta tccggcgcgc ttatgtctca tatgctacct attcgttgat
     2281 gtgttcgacg tcctcgtccg gcccgtcctc gtcccctgga gcgccggtat ctccaaaatc
     2341 ggccccgggg gaaagcggcg cgggttgccc gctcgggctc accagccgtc cctgtgcgtc
     2401 gaacttcatc ccgaaggaga gccccaggcc gccgagaatt tccttaattt cgttcagcga
     2461 cttgcggccg aaattcttgg tccggagcat ctccgcctcg ctcttctgca caaggtctcc
     2521 gatgctctga atattggcgt tcttcaggca gttgtacgaa cggacgctga gttcgagctc
     2581 ttccaccgag cggttgagga tctcgttcat ctggttcatg gcccgctcgg ccggctcttc
     2641 agcgacctcc ggcagctctt cgaaattaat gaagatcgcc atgtggtctt tcaacagctt
     2701 cgcggcctgg ccgatggaat cctgcggcga tatcgcacca ttggtccaaa cctcgagcgt
     2761 cagcttgtcg tagtcggtca tctgcccgag gcgcgcggcc tcgacgctga agttcacctt
     2821 ccgcacgggc gaatggaccg aatcgatggg aatgtagccg agcggaagat cttcatcgaa
     2881 gttccggtcg gcgctgacat acccgcggcc gatcttgaga cgcatttcga tattgagccg
     2941 gccgccttcg ccgatggtcg cgatgtgcat gttgcggtcg agcacctcga catcggcatc
     3001 ggtttcgatc tggccgctca tgacttcacc aggctgatcg acacgcagcc gtaccgtttt
     3061 caccacgtcc gtcatcatct tgaaaggcac ctgtttcagg ttcaggataa tgtcggtcgc
     3121 atcttcgaca actccgggaa tcggactgaa ctcgtgagcc acgccatcga tccgcacggc
     3181 ggtgatggca gcgccctcta tagaactcaa cagcacacgg cgtaaccccg taccgatcgt
     3241 ggttccgaac ccacgctcga agggctgagc ccagaacatg ccgtaacgct cggtaagggt
     3301 ctccgtattg gcgaccaggc gttttggctt ttggaagccc ttgaacataa agccttctcc
     3361 ttttacttcg aatacagctc gacgatgagc tgttcgttca actgaatctg cacgagttcg
     3421 tcgcgcttcg ggatactcag gacacgggcg cgcagggcct cccggtccac ttccagccaa
     3481 ttcggcgtcg gagcgtgcgc cgtggcgtcg cgtgcgccga gaatcgcggg attgttccgg
     3541 ctcttctccc gcacctcgac cacgtcgttt ggcttcacct ggaaggaggg gatattgacc
     3601 ttgcggccgt tcacctggat gtggccgtgc cgcacgatct ggcgcgattg ggcgcggctt
     3661 gtgccaaagc ccatccggta gatgacatta tccagccggc gctccagcat atcgagcatc
     3721 gtctcgccgg tgatgccctt tttcttggcg gctttctcaa agagattgcg gaactgccct
     3781 tcgagcagcc catagtagcg gcgggtcttt tgcttctcgc gcaattgcag gccgtagcct
     3841 acgattttcg gtctgcggct cttcccatgc tgccccggga aaaagttgcg tttctcgatc
     3901 gcacacttct ccgtatggca acgctcaccc tttaaaaaca acttcatccc ttcgcgccgg
     3961 cacaaccggc acacgggacc gttatatctt gccaatgtct gactctcctt acctgttttc
     4021 caggtgcagt ttacaactcc cggatgggag ccttcgatcc tgcttcccta acgcaatgct
     4081 tacacccttc gtttcttcgg cgggcggcat ccattgtgcg gtatcggcgt ggtgtctttg
     4141 atctgccgca cttcgatgcc gaccgtggcc agcgcgcgca ccgcggattc gcggccggcg
     4201 cccggaccgc tgacgcgcac ttccgccaca cggagcccat attctttcac tttattcgcc
     4261 gccgtcagcg acgcctgttg cgccgcaaac ggggtgccct tgcgggatcc ccggaagccc
     4321 agcgaaccgg aactcgacca cgcgagcacg ttgcccagcg ggtccgtaaa cgtaatgatg
     4381 gtgttgttga aggtggcctg gatgtgaacc acacccatgg gtacaacctt cttttccttc
     4441 tttttgaacg acttcttttt tcctgctttc gccggtgctt ttgccattcg ctctccgctc
     4501 gcttaggttt tcgccgaagc tttcttcttg ttggcgaccg tgccgcgccg tggacccttg
     4561 cgggtacggg cgttcgtgtg tgtgcgctgc cctctgaccg gtagcccccg gcgatgtctc
     4621 aatccccggt atgcgcccat ttcgatgagc cgcttcacgt tcatcgagag ttctttgcgc
     4681 agatcgccct cgaccgcacc ctccgtctcg aggatctggc ggatgcgatt cacctcgtct
     4741 tcactgaggt cgacgacttt cttgttggcg tccacgcccg cgcggtgaag gaccgatttc
     4801 gaccgcgtgc gcccaacgcc atagatgtaa gtgaggccga tctccgcccg ctttctgttg
     4861 gggagatcga cgcctgcaat acgtgccata gtccctcttc ctatccctga cgttgcttat
     4921 gtttcgggtt cacacagatc acccggacca cgccttcgcg acggatcagc ttgcacttgt
     4981 cgcacatttt cttgactgaa gctcgtactt tcattttcgc tttcccggcc ttgcccgacc
     5041 cggcgtctcg ccggatggcc gccttttaac gcttcttcag aagctcaata atcctgcccc
     5101 gggtttcatc ttcgagactg ggttccacct gaaccctgtc ccctacccgg agacgcacaa
     5161 aattgcggac ctgtgcgctc gccaagtgcg ccagcatctg ccgccggtcg ctcaactcaa
     5221 cccggtaagt cgcgctcgga agcaactcga tcactactgc ctcccgcgac cggttctccc
     5281 gctcggcgcc ctcgtccatt cccggcgttc agggcctcgt cagtacccac ggcccattcg
     5341 aggtcaccgc cacgcagtgc tcaaaatgcg ccgagtaaga cccatccttt gtgacagcgg
     5401 tccacttgtc cgacgccagc cgcgattccg ctccccccgc attcaccatc ggctcgatgg
     5461 ccaggaccat cccttccttc aagcggggat tctcgttccg gcgatcaaca taattcggaa
     5521 cctgcggctc ttcgtggagc cgggtaccga ttccatggcc cacatactcc cggacaatac
     5581 tgaatccgtg ggaagtcacg tgcttctcca cggtcccgca aatatcgaac aggcggttgc
     5641 cgacccgcat tttgccgatc gccagttcca acgactcgcg ggtcaccgtc agcaattttt
     5701 tcgtctcgtc cgggatatcg cccaccggaa ccgtgatcgc cgaatcgccg aaatacccgt
     5761 ccaactgtac tccggtatcg attgaaacca cgtcgccttt cttcaacacc cgcttcgtag
     5821 aaggcatccc gtggacgatc tcgtcattca ccgacgtgca caacacgtag ggatacttgg
     5881 aacctgccgc cggaacgtag tagcctttga aagccggccg ggctcccgcc tccgacatca
     5941 tcttttccgc gatcgcttcc aaatcccggg tggtcacacc ttcagcgacc accccggaga
     6001 gcttctgcaa aatctcccaa accagcagcc ccgcgcgccg catcttttcc agttcggccg
     6061 ggctcttacg aataatcatt ctcccctgat atggtggcag gcccgctccg cgatatcggt
     6121 aggcgaaccg tcgttgccat ccacggtata aaacttcgaa gccaattggc ggaagtattc
     6181 gaccagcggc agggtctgcg cctcgtacgc ctcaatccgc tgccggatca ccgtctcgca
     6241 atcgtctccc cggagaatca atccggcacc gtccccatcg cacacgccat cgctcttcgg
     6301 aggcttggag atgacgttgt aaacggcccc gcattgagga caagaccggc gagccgtcag
     6361 acgggaaaca attctattgt aatccacttt caaatggatc accaagggct ccaggccctg
     6421 ctcttccagc atcccggcga gcgctttccc ttgttcgagc gtccgggggt atccgtccag
     6481 gatgaagcca tccaggcagt cgtctcgcga aatccggtcc tgaaccaggc ggttcaccat
     6541 tccgtcggga accagcccgc cgttttccat caggctttgc acctgcctgc ccaattccgt
     6601 accggcctgc acgttttccc ggagaatgtc gcctgtcgaa atatgaggga tatcaaagca
     6661 acccgtcaga tgtttgccta cagttccttt cccgcaaccc ggggggccga acaacaacac
     6721 cgccacggtg cggataccga tcttcggcat atcaactcac cttccctatc ctcgtaagcc
     6781 gcggaccgtc ccggaacagt cagaacagcc tcacggtcag aaagtgctcc tccgcccgcg
     6841 tatccggccc gcgcgcggag tgaacccttc gtagtgccgc ataatcagtt gcgcctccac
     6901 ctggttgacg gtgtccatgg caacgcccac cacaatcagc aaagaggtac caccgaaata
     6961 gaaattcacc cccaagccgt ccagcaacca ccgtgggaaa taggcatcga tgaaattgcc
     7021 ggccagaggg aggtgttgca gcttgatccc ggagatcatg atctgaggta tcagagaaag
     7081 aagtgccagg tacaatcctc cgaccatggt gatctttgtc aggatcttat tcatatattc
     7141 ggcggtgttc ttcccgggcc ggatccccgg cacgaacccg ccgtacttcc gcatgttatc
     7201 ggctgcttcg ttcggattaa aaataataga aacgtaaaag aagcagaaga aaacgatgcc
     7261 cagcacgaac aaaagagcat gaagcggctc ggcataaccg agcgattcga gcattcctct
     7321 cagccaccgg ttgtcacgga atgccggcac ctgtaagagg gtctgcggaa acgcgagcaa
     7381 cgaggaagcg aaaatcacgg gaatgacgcc gccggcgttc actttgagcg gcaggtgcgt
     7441 ggattgtccg cccatcatgc ggcggcccac cacgcgcttg gcatactgca ccggaatgcg
     7501 ccgctcgccg cgctccacca gcacgataaa cgcaaccacc gccaccatga ccagcagaat
     7561 gatgatcagg tgcagcgcgc tccagttgcg ggtgacaaaa acgttgttat aaatctccgt
     7621 gatggcgtgc ggtaagccga ccacgatacc ggcaaaaatg atgagggaca tcccgttgcc
     7681 gataccgcgc tcggtaatct gctcgccgag ccacatgatg aaagcggtgc ccgtcgtcag
     7741 gctgagcatc gtcatcagca caaagccgat tccggggttc aatacgaaat tcccctccat
     7801 cgactgcagt cccgtagcga ttcccaacga ctgcaacaaa gccagaataa tcgtaagata
     7861 gcgggtccac tgcgtaatct tgcgccgccc gagctcgccc tctttctgga gtttttcgag
     7921 cgtgggcacc accacggtca tcaactgcag aatgatggat gccgtaatgt acggcatgat
     7981 gcccagggcg aaaatcgcca tttgcgaaaa catgccgccc gaaaagaggt cgatgaatcc
     8041 gagcagcgtt cctttgttct gctgaaaagc ttcctgcagg cgtgccgcat cgatgccggg
     8101 agtggggata tgcccgccca gccggtaaat cgccagcatg gccagagtga acaagacccg
     8161 gttgcgaaga tcgggaaccc gaaatacgtt cgcaaacgcc tcgaaaaact tcatgatatc
     8221 gctcgatttc cgcctatttc gtccggccga cgacttccac gctaccgccc gccgcctgga
     8281 tcttctccgc ggccgccttg gaaaagaggt gggcctgaac ccgaatcgcg cgcttcagct
     8341 cgccgtcacc caggatcttc aacccgtccc ctaatttctt caccacaccc aactcgacca
     8401 ggcgggccgg gtcgaaggaa tcgccctcta gctcgccgag atgaccgaca tttacgatgg
     8461 cgtactcctt cttgaaaata ttcgtgaagc cgcgtttggg catacgccgg tgcaagggca
     8521 tctgcccgcc ctcgaaaccg cgcatctgat tgaatccgga aaccgaatgc tgccccttct
     8581 gtccgcggcc tgacgtcttg ccccggccgc tccccatacc acgcccgatt cgccggcggg
     8641 agtgcttctg tccggcggga ggcttcaatc cactgagatt catacggtcc cctattcgac
     8701 aactgcgagc agatgcggca ccttcgccac cattccgcgg aaggaaggac tgtcgggccg
     8761 ctccaccacc tgattgatct tcttcagccc gagcgaacgc acgatcttct tgtgcttttc
     8821 gggcgtgcag atcggactcc gaaccagctt gatcttgatt ttaccttcag acattgctca
     8881 atttcctgat gggcctgttc gacgagtatc cagtttttcg acggccacgc cgcggaggtc
     8941 cgccatcgcg gtccggtctc gcagttgctc gaggccctca atcgtagcct tcaccacgtt
     9001 gtgcggattg cgggagccga tcgatttcgt caggacgttc ggaataccca ccgcttgaat
     9061 cacggcgcgc accggtccac ccgcgatcac gccggttcct tcgggcgccg gcttcagcag
     9121 cacgagcccg ctgccgaacc ggccgacaac ctggtgcgag atggtggtct cgaggacgtt
     9181 gatcttgcgc aggttcttct tggccgcttc gatgcccttc ttgatggcgg agggaacttc
     9241 cttggccttg cccgttccga atccgacgac cttcgccccc gcgtcgccga caacaaccag
     9301 agcggaaaag ctcaggttct tgcctccctt gacgaccttc gttacacggt tgatcgatac
     9361 cacctgctct ttgaggttga acaactgcgg atcgaccttc ttcactgccc gcatggttcc
     9421 catcaaaact ccaatcccgc ttcgcgtgcc gcgtcagcca gcgctttcac gcggccgtga
     9481 tagatataac cgccacgatc gaacaccact ttggtgacgc ccttttccct ggcccgctcc
     9541 gccaacagct tgcctacctc tttggcgccg ctcacgttgc cgccatttcc cgcggcgctc
     9601 ttctcgcccg aggaagcggc agctaaagtc cggccttcgc ggtcgtcgat cacctgcgcg
     9661 tagatgtgct tcagactgcg gaatacgaca agtctcggcc gctccggcgt cccgcgaatc
     9721 cccttgcgga tgcgccggtg aatccggata cggacgttgt tcttcgatac cttgcgaatc
     9781 attcctcgat tgcctccgga ctacgcataa cggcctactt gcttccggcc ccggtcttgc
     9841 cgaccttctt ccgcagaact tcaccggtat accggatacc tttgttcttg tacggctccg
     9901 gcgggcgcag cgcacggatg tcggatgcca cctgtccgag caactgcttg tcgatcccgc
     9961 tcagcgtgag atgcgtctgc ttgtccactt tcaggtccac cccatcgggc agaagaaact
    10021 cgatcggatg agaatacccg agggcgaacg tcgcaatctt ccccttgaca tcgcagcgat
    10081 atccgatgcc cacgatatcg agctctctcg tgaatccgcc cgatacgccc tgtatggcat
    10141 tcgccgccaa tgcgcgcgtc agcccgtgca gggcggcgta ttcgtcgccg tcccgggcaa
    10201 tctccacatt tccgtcgtcc tggcggataa ccactcccgg cggaatgggt acgctcagct
    10261 ttcccttggg gccttccacc tgcaattcgc ccgcggcggt gatcttcacg cctttcggca
    10321 acggaatcgg cttttttcca actcgtgaca tgcttccctc tccagtcaac cggtcttcaa
    10381 ggttgactgc tcgcaccgcc gcgcaggcgg ctcaatagcc cgatggcgcc cacgcttgcg
    10441 aagagcggct accacacctg gcacaaaatc tctccaccca cgccctcttt ccgggccgtg
    10501 gaaccggtca tgacaccccg cggcgtggtc aggatgttaa tgcccatgcc acccaaaacc
    10561 cgtgggatct ccttacttcc ggcatatacg cggcagcccg gacgggacac gcgctccagg
    10621 tgcgacatca ctggaacgtt gcccggcgta tacttcagat aaaccctgat gacgccccgt
    10681 atcccttcgt cggccagctt gtagttgaga atatagccct cttctttcag aatgcgggcg
    10741 atctccacct tgagcttcga accgggcaca tccactttcg gatgccgcgc gatttgcgca
    10801 ttgcggatcc gcgtcaacat atcggcgatc ggatcagata ccattctgtc ttaaccctcc
    10861 gatacctacc agctagcctt gatgacaccg gggatctctc cagccagcgc caacccccgg
    10921 aaacagatcc ggcagagatt gaacttgcga agatacccgc ggggccgccc acaccggcgg
    10981 cagcggttcc gatgccggca gcggagcttt tttgttctcg cctgcttggc tttttcaagc
    11041 ctcaggtccc tagcgatttt ggccgttgtt gccatccacg atcccttcaa tttcctctat
    11101 tgacgaaaag gcataccgag ctgccgcagc agagccagac cttcggcatc cgtcctggcc
    11161 gtcgtcgtta tacagatatt cattcccttg atcttctcga ccttgttgta gtcgatctcg
    11221 ggaaagatca actggtcctt gatccccagc gtgtaattgc cgcgcccgtc gaacgatttc
    11281 gtggacacgc cccggaagtc gcgaacccgc ggcagggaaa cgttcaccag ccggtcgaag
    11341 aactcgtaca tgcggtcgcc gcgcagcgtc accatgcaac ccaccgacat gccctcgcgc
    11401 agcttgaagg ccgcgatgga tttactcgcc ttcgtcacga ccggcctctg accggcaatc
    11461 tgcgccaact cggccaccgc cggatccatc agcttcgagt tctgggtagc ctctcccaaa
    11521 ccgatgttca aggagatctt ctccagcttc ggaacggcca tcacgttctt atagccgaat
    11581 tccttgacca accccggaat cacgttcttc gtgtaatgct ctctcaacct cgcgcccata
    11641 gcactattcg tccatttcct tcatcccacg ctccctcgcg gccgcggctc cgaagccggt
    11701 actgagccgc gcgagtgagc aagcggtcac tagcttttct tatccagaac ctcaccggtc
    11761 ttcctggcga tgcgcacgct ccgctggccc gcgccggtcc cttccacacg gtagccgatg
    11821 cgtgtagcca caccacccga agtcaccacc atcaggttgg aaaggtggat ggtactttcc
    11881 cgttcggcga tgccgccctt gatctgtttg gaaggattcg ggcgggtatg ccgcttgatc
    11941 agcatcacac cttccaccag cgcccttcct ttttcgcgat cgacgttcaa tacacgcccc
    12001 gtcttgccct tgtcgcgccc ggtgatgact ttgacgatgt catccttctt gatcttggac
    12061 ttgatccggg tcttcgggac gccctggtgc cgcggaggcg tcgctttctg ctgctgccgc
    12121 gccatcagat cacctccggc gccagcgaga cgatcttcat gaatcgcttg tcgcgcagct
    12181 cgcgagccac cgggccgaac acacgcgtgc cgaccggctc gcccgtctcg gtaatcaaca
    12241 ccgcggcgtt cgaatcgaac cggatatagg tgccgtctct gcgccgtgtc tctttccgca
    12301 tccgcacgat cacacaccgg acgaccttgc cttttttaat attggagtcc ggcgcggctt
    12361 ccttgacgga agcggtaatg atgtcgccca atccggccct cagcccgagg tcgccgccgc
    12421 gcggcagaat acaggagagc cgtttggctc cgctgttatc cgccacgtcg agaatggttc
    12481 gcattccgat catagggatg tctccttctc ttcctcttcg agagtgccaa cctgcgccgc
    12541 gcgcctcaac acttccgcaa ggttccagcg cttcagcttg ctcaacggcc gcgattcgat
    12601 gatgcgcacg atatcgcccg gcctggcggt ctgctcttcg tcatgcgcgt agaacttctt
    12661 gcgcctggtc acaatccgct tgtacagcgg atgcgggacc cgccgggtca cctccaccac
    12721 gatggtcttc tgcatcttgg tggaaaccac ctggccggtc ttctcgttcc ggattccggc
    12781 gacgggtgct tccgtcatgg ttatctggcc cccttccggc gccggtcccg accctgtccg
    12841 gccagcgcgc gctcgtgcag aatggtcaac atccgcgcgc ggtctttccg gatctgccgc
    12901 agcttcttca gtccgtccgc ctggcccatc ccgatctgga acttcagccg gaaaagctga
    12961 tcttccatct ctctcagctt gatgttcagc tctttgctgt cgagttcccg aaccttttcc
    13021 gctctcatct gctactccac cctctcgcgg gcgaccatct tacacggcag cgggagtttc
    13081 tgcgccgcca gccgcatcgc ttcctccgcc acatcgcgag gaacgccttc catttcaaac
    13141 agaaccttgc ccggccgcac caccgctacc cactgctccg gcgcgccctt gcctttaccc
    13201 atcctggtct cggccggctt cttggtaatg ggcttatccg gaaacagacg gatccagacc
    13261 ttccctccgc gcttgatgaa acgggtcatg gcgatacgcg ccgcttcaat ctggcggtcg
    13321 gtaatccagc cgcattccat caccttcaac ccgaaatcgc cgaaggaaag atcggagccg
    13381 cgccaggcct taccgcgcat gcggccgcgc tgctgcttcc tgtgcttcac tttcttgggc
    13441 atcaacatgg cgatatctcc cttctacgct ccgattactg ctgctctccc ggctccggac
    13501 cctcgcctgg tttcccggcc tcgggctccg ccgccggctc cggctcctgc ttcagttcct
    13561 gtttccacgc gggcagcggc ggcgccagcg gcggaaggat gggcgcggtg ctgcgcgccg
    13621 gctgcggcaa ttcgcccgga gcctgttgtt ccaccggacc ggcctgtgcc gccggagcct
    13681 gggaaggcaa cggtgccggt ccccttcgtt cgccccggtc ccgatgatcc cggtcaccgc
    13741 cgtgtccccg ttcccggcga tcgcgctgcg gccgccgcgg ctcgggttcc ggtgtgaaag
    13801 ttcctcgctt gccgtccaga atctcgccct tataaaccca ggtcttgatt ccgataacgc
    13861 cataggtcgt gtacgcctgc gcaaatccga aatcgatgtc ggcgcgaagc gtgtgcagag
    13921 gcaactggcc ttgcaggtac cactcgctcc gggcgatctc cgctccgttc aaccggccgg
    13981 atacccggac cttgatccct ttgcagccga accgcagggc ggaatccacc gccttgcgca
    14041 tcgcccgccg gaacgccaca cgcttttcca gttggagcgc gatcgactcg gccacaagct
    14101 gagcatccag ttccggcttg tgcacttcct gaatgtcgat gaaaatatcc cgcttggtct
    14161 tccgggcgag atcctgcttc agcttctcga tctcggcgcc tttgcgtccg atgatgatgc
    14221 ccggccgcga cgtgcggatc gtgatgcgca gtttgttgcc cggccggtcc acttccaccg
    14281 aactgacacc ggcggatttc agccgcgttc gcaggttctc tttcaactcg agatcttcga
    14341 gcagcagttt cgcgtaatcc tgcttggaga accagcggga tttccaggtc ttggtgaccc
    14401 cgagccggaa tccgtaagga tgaacttttt gacccatgat ctactcttcc gtcttccggt
    14461 cgccgaccgc gaccacaatg tgcgcgctcc tacgctgata ccggaatgca cggcccatcg
    14521 gggccggccg gatgcgtttc attcgcggcc cctcgttcac gtaggcctgc gtcaccaaca
    14581 gcgcatccac gtcgacatcg ttaaagcggt tttccgcgtt gctgatcgcc gatcgcaaaa
    14641 ccttctccac cgccggtgcg atccctttat tcgtggaccg caggatggtg atggcatccc
    14701 ccgctttccg gccgcggatc tggtccacga ccagccgcgc cttctgcggc gagaccctga
    14761 tgtacctggc ttccgctctc gcttccataa cactctcttc ctaggagggc ctcgacgact
    14821 tctcggtctt cgccgcgtgc cccttgtagg tccgcgtggg tgagaactcg cccaacttgt
    14881 gtcccaccat gttctcggtg atgtataccg gaatgaactt cttgccgtta tgcaccgcga
    14941 tggtgtggcc gatgaactcc ggcaggatcg tggaacggcg ggaccacgtc cgcaaaacct
    15001 tcttttcgtt tttcgagttc atcgcttcca ccttcaccat caggtggctg tccacgaaag
    15061 gtcccttgct taaagaacgt cccatatgct atccgccgct acctcttctt cgaccggtgg
    15121 gtgaccaccc acttgtccgt acgtttgtta ttccttgtct tgaagccgcg cgtgggctgg
    15181 ccccagggcg tcaccggatt ccggccgccc gacgtccggc cctcgccgcc gccgtgcggg
    15241 tgatccaccg ggttcatcgt cacaccccgg ttatgcggcc gtttgcccat ccaccgcttc
    15301 cggccggcct ttccaagcga aacgttctcg tgatcgaggt tgcccacctg gcccacggtt
    15361 gccatgcact ccagttgcac acgccggacc tcgcccgaag gcagcttcag cagcgcgtat
    15421 ccgccttcct tcgacaccag ttgcgcctgc gagccggccg aacgcaccat ctgtccaccc
    15481 ttcccggggc ggagctcgac gttatgcacg aacgttccgg cagggatctg ctgaagcggc
    15541 agcgcgttgc ctaccaggat gtccgcttcg ggtccggaga ccacctttcc acccacctga
    15601 agcccgacgg gagccagaat gtaccgcttc tcgccgtcgg cataattcag cagcgcgata
    15661 cgggcggagc gattgggatc gtattcgatc gccgccaccc tcgccggcac accggccttg
    15721 tcccgcttga agtcgatgat gcggtatgct ttcttcgctc cgccgccgat gaatcgcgag
    15781 gtgatccggc ccagcgcgtt ccgtccgccg ctgcgttttt ttccggtcac cagagacttc
    15841 tccggcttgt ccttcgtgat ctcgttccgg gaaaccaccg tctggaatcg ccgggtcggc
    15901 gtcgtcgggc ggtatgtctt tatcgccata tcgctgtcct cagatctcgg cgtactccgg
    15961 catcttctcg ccgggtttca gccttacgta tgccttcttc cagtccgggc ggtatcccgt
    16021 aaaccggccc cgccgccgca gcttgccttc ctgggttgcg gtccggacct cttccacctt
    16081 cactttgaaa agctgctgca ccgcctggcg gatctgggtc ttgttcgcat ccacggccac
    16141 ttcgaagcag agcgtccgct cgttgtcttt cttctcaaca cccttttccg taataatggg
    16201 gcggcgaatc acatcaaagc ggttcataaa gccagcgcct ccgacaattt cctggccgcg
    16261 ctctccgaca gcaccacctg cgtatgcccg agcagatcgt aaacggtcac ctcgcggctg
    16321 gcgaccagcg tcacacccgg caggttccgg cagccaagct gcaggttgcg gttctcttcg
    16381 ttctcgacca gcagaacctt ccgccccgcc tcgatctttc cgagcgccga ccgcatggac
    16441 cgcgtcttgt ggtccggcag cgtgaacgcc tggatcacct tcagttcgcc gtcgcgcagc
    16501 ttggccgaca gcgccgaacg cagcgcgccc agcagcatct tgcgcggaag cttgtaggag
    16561 tagtcccgcg gctgaggccc atgtgctgta ccgccgtgcc tccacagcgg cgagcggatg
    16621 gagccgacgc gcgcgcggcc ggtccccttc tgccgccaca gcttcttgcc ggaacccgac
    16681 acttcccacc gcgtcttcgt cttcaccgtg ccgctgcggc gggacgccat gtagtggcga
    16741 acagcctcat acatcagagc ttcgttgacg gcggcgccga aaaccgaatc ggccagttcc
    16801 aactcgccga ccttttgatt attcagatcg aaaacatcaa tactcggcat tcccgctacc
    16861 tcttcgcccg ccggaccacg acatagccgc cgttcggccc ggggaccgcg cctttcacca
    16921 tcagcacgtt gtcttcggca tcgattccca cgatctccag gttccggacc gtcacctgtt
    16981 gcgtgcccat atggcccgct cctttcatgc ccggaaaaac ccgcgacgga tagctcgaag
    17041 cgccgatcga tcccggagcg cggtggaaca tggagccgtg gctcccttcg cctccgcgga
    17101 agtgatggcg cttcacgaat ccggcaaaac cccgcccctt gctgataccg gtcacgtcga
    17161 ccttttgcgc gggcgcaaac tggtccacca gaatgcggtc ccccggcttc aggtcgtcgt
    17221 cgcccggccg gatacgaagc tctttcgtaa acttcacacc ctcgacattg gcctttttca
    17281 aatggcccgc ggcaggtttc gtcagcctgg acgccttcac gaactccatc agaccgagtt
    17341 gcacggcatc gtagccgtcc acaaccggtg ttttccgctg taccacgacg cacgggccgg
    17401 cttttaacaa agtcaccggc accacttgtc catcaggccg gaacacctgc gtcatcccga
    17461 tcttcttgcc tagaattcct gggctcatac actcacctgt cgtcgctggt tcgcttcctg
    17521 cggccccctg ggggccccgc tatcaggcaa cccggcgacc tacttgcctt ttccgaacgc
    17581 tttgatctcc acatccacgc ccgcgggcag atccagcttc atcaacgcgt cgaccgtatc
    17641 ctgcgtgggc tcgagaatat ccagcagccg tttgtgagta cggatctcaa actgctccct
    17701 ggacttcttg tctacgtggg gcgagcgcaa aacggtaaaa cgattcttgc tggtaggcag
    17761 cggaatcgga ccggcgatct gcgcaccggt tctcttcgcc gtctccacga tctcggtggt
    17821 ggactggtcc agaacccggt ggtcgtacgc ctttaagcga atgcgaatgc gttctctaag
    17881 cattgtttcc tgtccgagcc ggacattgcc ggcccgctta acgagttggc ccgagcctcg
    17941 aaccgaactc tgtccggtcc cgggccaacc ttcggtgcct gctactccag gatctccgtg
    18001 accgttccgg cgccgaccgt gcggccgcct tcgcgaatgg cgaaacgcag tcccttgtcc
    18061 atcgccaccg gcgtgatcaa ctccacttcc aggttcacgt tgtcgccagg cattaccatc
    18121 tcggtccctt ccggtaactg cgccactccc gtcacgtccg tcgtccggaa gtaaaactgc
    18181 ggccggtagc ccttgaaaaa cggcgtgtgc cgcccgcctt cttccttgct caatacgtac
    18241 acttctcccc ggaacttctt gtgcggcgtg atcgaccccg gcttggcgat cacctggccc
    18301 cgctccacgt catccttgtc gatgccccgc agcagcagtc ccacgttgtc gcccgcccgg
    18361 ccttcgtcga gcagcttctt gaacatctcc acgcccgtca ccaccgtctt gcgcgtgtcc
    18421 cggaaaccga cgatctccat ttcctcgccg accttcacga tgccccgctc gatgcgcccc
    18481 gtcaccaccg tgccccggcc ctggatcgag aaaatgtctt caatcggcat gatgaacggc
    18541 ttgtcgatcg cacgctccgg caccggaatg tagttgtcca ccgcctccat cagctcgtcg
    18601 atcttctttt cccactccgg atccccgttc agcgcctcca gcgcgctgac ccgcaccacc
    18661 ggcacttcat cgcccggaaa ctggtagctc ttcaacagct cgcgtacttc caactccacc
    18721 agttccaaca gttccgcgtc gtccaccgcg tccactttgt tcagcgccac cacgacgtag
    18781 ggcactccca cctgccgcgc cagcagaatg tgctcccgcg tctgcggcat cggcccgtcg
    18841 gttgccgcca ccaccagaat cgccccgtcc atctgcgccg cgccggtgat catgttcttg
    18901 atgtagtcgg cgtgacccgg acagtccacg tgcgcgtaat gccggttcgg cgtctcgtat
    18961 tccacgtgcg acacggcgat cgtgatgccc cgcgccttct cttccggcgc gttgtcgatg
    19021 gaatcgaagc tccggaactt caccttcgga ttatgcttcg ccaggcactt ggtgatcgcc
    19081 gctgtcagcg tcgtcttgcc gtgatcgata tgcccgatcg tccccacgtt tatgtgcggc
    19141 ttgctgcgct cgaatttctc cttcgccata tctcggaatc tcctctgcct ttctggatcc
    19201 tgttagacct acctggtgac ctttccctga accctgctga cgatctcttc ggagatcgcg
    19261 gccggcactt catcgtagcg gccgaagtgc atggtaaaac ttccgcgccc ctgcgtccgc
    19321 gaccggagct cggtcgcgta tccgaacatc tcggagagcg gcaccatcga cttgatgatc
    19381 tgcgacgttc cgcgaagctc gatgccttcg atgcggcctc tgcgcgaatt cagatctccg
    19441 tagacatccc ccgtgtactc ttccggaact acaacctcga cggccatgat gggctcgagc
    19501 agcaccggct tggctgctgt ggccgcggct ttgatcgcca tggacccggc gatcttgaag
    19561 gccatttccg aggagtccac atcgtggtag ctgccgtcat acagtaccgc cttcaaatcg
    19621 gacatcggat agccggccag cactccgcct tccagcgctt ccaccatacc cttctcggcc
    19681 gcggggatat actctttggg aatcgacccg cccacgatgg cgttctcaaa ctcaaacccg
    19741 gtacctgccg ggagcggctc aacgcgaatc ttcacgtgcc cgtactggcc tttgccgccg
    19801 ctttgccgca caaatctgcc ttcggcttga gacggcttgc gaatggtctc gcggtaggca
    19861 acctgcggct tgccgacgtt ggcgcccact ccgaattcgc gctgcatacg gtcgacgatg
    19921 atttcgagat gcagttcgcc catgccggcc agaatggtct ggcccgtatc cggatccgtc
    19981 gagaccttta aagtcggatc ttccgtcacc agcttggcaa tcgccatgcc cagcttttcc
    20041 tggtccgctt tcgtcttggg ctcgatggca agctggataa cgggcgtcgg gaaatcgatc
    20101 gattccagca gaatcggcat cttttcgtcg cagacggtat ctcccgtcac taccgtcttc
    20161 aaccctacgg ccgcgacgat atcgccggca tagacgtcct gcacttcttc ccgcttgttg
    20221 gcgtgcatct tgaccaggcg tccaacgcgc tcccgcctgc ctttggccac gttatagacg
    20281 gactcgccgc tgaccacgcg ccccgagtac acccgcagaa acgccaactg gccgacaaag
    20341 gggtcggtca taatcttgaa gatcagcccg gcaaacggct cggcgtccga ggactcccgc
    20401 accataattt cgtccggatt gtcgatggcc gtgccctgca ccggcggaac ttcggtcggc
    20461 gaaggcaggt aatccaccac cgcgtcgagc aggttctgca cgcctttgtt cttgaacgaa
    20521 gagccgcaga tcaccgggaa aatcttcagc gcgatcgttg cgcggcggat gcccgtgcgc
    20581 agctcctcgt tggtaagcgg ctgtccgttg acgaactttt cgaatagcgc ctcatcggac
    20641 tcggataccg cttccaccaa ctgttcgcga tactcttgcg ctttggcggc cagatcctcc
    20701 gggatcccct gatcgtcgaa tttcgcgccc agggtctctt cgtgccagat acgggcgcgc
    20761 atctccacca gatcgatgac gcccttgaag tgatcttcgg aaccgatcgg gatctgaatc
    20821 ggcacgggac ggcacttcag ccgctgcaca atcgtgtcca cggcacgata aaagtccgct
    20881 cctatccggt ccatcttatt gatgaagcag attcgcggaa cctggtactt atcggcctga
    20941 cgccacaccg tttccgattg cggctggaca ccggcgaccg cgtcgaaaac cgctaccgct
    21001 ccatcgagaa cgcgcaggct gcgctccacc tccgcagtga aatccacgtg gccgggagtg
    21061 tcgatgatgt tgatctgggt gtcgcgccac tggcaggttg tcgcggcgga agtaatcgtg
    21121 attccgcgct cctgctcctg ctccatccaa tccatcacgg cggtgccttc atggacctca
    21181 ccgatcttat aggtgcgtcc cgtgtaatac aggatgcgct cggtggttgt cgtcttaccg
    21241 gcatcaatgt gggccatgat gccgatattg cgcattttct cgatgtcgac cgttcgtggc
    21301 ataaaaacgc tacttgcccg tcagaacctc cggctaccag cgataatggg cgaatgcctt
    21361 gttggcctcc gccatgcggt gcacgtcgtc cttcttctta attgctcctc cacgcatgtt
    21421 cgcggcatcg ttcagctcgt tggcaagctt ctccggcatg cctttctcgg cccggccgcg
    21481 ggcattcaaa atcagccagc ggatggcgag gctggtgcgg cggttctgcg gcacctcaat
    21541 cggcacctgg tagttcgcgc cgccaacacg ccgcgttttc acttccagca aaggcttgca
    21601 gttctcgacg gccttcttga acagcttcag cggatcgtct cccgtcctct cccggatggt
    21661 gtccatagcg gtatagaaga cgccttgtgc aaccgtcttc ttcccctgcc acatcatgga
    21721 gttgacgaac ttctccacca gggtggagtt gtagaccggg tcgggtgcga tctctttcaa
    21781 ttcgggtctg cgtctgcgtg gcatagttct aaattacgat ttcggcctct tggctccgta
    21841 cttcgaccga ccttgtttac gattcgccac gccggcggcg tccagcgtgc cgcgaacgac
    21901 gtgatatcgc acgcccggaa ggtccttcac acgtccaccg cggatcagca cgatggagtg
    21961 ctcctgcaaa ttgtgtccaa cccccggaat ataggtagtg acttcgattc cgttggtcag
    22021 gcggacgcgg gcaaccttac gcaaagcgga attcggcttt ttcggcgtcg tcgtatagac
    22081 gcgcgtgcag acgccgcgcc gctgcgggca ttgttgcagc gccggacttg ccgtcttgta
    22141 gcgctgcagc ttgcgccctt ttcgcacaag ttggttgaag gtcggcacga aaaatctatc
    22201 tccttatcgc ggcacccggt tgcacattgc accacaatcc aggcgcattc ttaacgctat
    22261 ttgctcgaaa tgggctgccg atcgacaatg gggcaccttt caaattcacc ttcacggcga
    22321 agggcctgtc ggtcaaccgc ttaagaaagc cggcccgcct ggaccgcttt cagttgcgag
    22381 ggttacttgc ttcggactcg cccatgctcc gccggcttcg atatccgccg gaacctcggg
    22441 gctgacccct ggccggactc ctcaacacaa aagagtggta gcacaggagc agaagaattc
    22501 aactcgccaa accttattag aataacggag cgcagaaatc ctgtcaacct cctccaaccc
    22561 ggctattgcc gaagcgtgaa gccgagcacc tccactctgg cgctgcgcgt gcttcgaggc
    22621 atatgcagaa agaactgggc gtggatttca tcgaagtcgt cgatcacgga acttgcgggc
    22681 atcgcaaagt gcaaagttcc ctgcaaacgt cccgcggcga ggcctttctc cggcggctgg
    22741 agttcgctga gttcttgggt gcccaggctc tgccggcgga agcgcccttc ctctcccgtc
    22801 cgaacgagat acatgtccac gctcaccgtc gaggcttcct ggggaagtgc ggaaacttcc
    22861 acttccacgg agttgcaaca ggacatcgaa agctgagtgc cgagcggctg gcgcgcaatc
    22921 atggtgagag gatgacgatc catgttggta aacagccact ccatgggaga accgaactgg
    22981 atcatcggcg ttgcggatgg ccggctgctg agccaggaaa ggaaccagta ttcaccgctg
    23041 aacgggattc tggtgctttt gcgggcgagg gccggcgtcg cgcttggggg gcgctccaga
    23101 cggggcgcga tcaaaactac gtgccgcggg tttttgctct tcagaattac gcttgagagc
    23161 aagccgctat ccgcgcgaac cgcggccttc ccttggctcc caccagtcct cgatccggga
    23221 taaagcggcc gcctcccgaa tccgccgatc caggccatca acgcaatcgc acacagaacg
    23281 gaaacgactg aacggctggg acgaaatctg atcggagtaa aattgtcttc aacttttctc
    23341 aggctccagg cgaggaacaa gcctgccgcg gcaaacaaga gcgctccaag gattttgttg
    23401 ccgaacatca gcgcggcaat cccggactgg atgcataacg acagcccgag agcgcaccgg
    23461 ctccagggag gaggacgctc tgcaagactt gcgaaaatct cagcgaaaac cggcgtagat
    23521 ggcagatcaa tgccggtctc catgccggat gcgagcaacc ggcgatagcg ctgaaccagc
    23581 cggccaaatc caatcgcgag ggcgcccgat agaagcgcca gccaaaccgc cccattccag
    23641 gtcatcagaa tcagaaccag aggtgccatc cacgcactcc atccggcaaa cgcaagtgca
    23701 acgttcagcc ggggagcgtt cgagtttatc cgggtgaacc ggtaggccag gagcgccgcg
    23761 caaagcgatg caagaaacac cgacaagacg gcaaatgtgg gtcgcaggat cgctttcggg
    23821 aaggactcaa ccggggaacg gaaaatgtgg tttacagcta aggcgagggc ggccgcaacc
    23881 gcagccgatg ccagcagtgg actccaatcc gccaccggag aatcccgcca ttcgctgcgg
    23941 gtttcgaaca tccgcggcac ctccccggga cctgcaattg tccttcttag acaaatgcac
    24001 cgctgataaa gttcccatac tgggggcgga tgtattggga cagaacggcc cggaagcgga
    24061 gctgcgcgcg gctactctac ttccattccc accggagatt cggactcttc cggggaaacg
    24121 ggcgattgcg tcagcccgta ctcgtagcct ttgtcgaacg ctttcaggtt caggtctttg
    24181 aacgaagggg gcacggagtc cgcaacggct ttgcggagcg cgtcggggtt gaggacgccg
    24241 gaaacggcgg caaagaagcc gaccatcacg atattgagca ccagccgttt ccccaactgt
    24301 tcggccagcc gcgtggcggg cacgctgtag acccgaatgc cggcccgaag gttatcgaca
    24361 cgcaccaggt cttcttccac cagcagaagg ccgccatcct tcaactcggg cacgaacttg
    24421 ttgaaggcgt cctgggacat ggcgatcagc agatcaggct gtgtgatgta gggatacagt
    24481 accggctcgt tcgatagaat cacctgtgcg ctgcaagcgc ttccgcgcgc ttccgggccg
    24541 aagttctgcg tgagcgtggc gtagccgtct tcaaaaatag acgcggcttt tccgatgatc
    24601 attgcggcca ggatgactcc ctgcccgcca aaacccgcca cacggatctc ggtcagatct
    24661 ggcatggggc ctccaggggg tcaacatact cctcgtgaag cttgcttcgc atctgcgtct
    24721 tcatcacatc gagataatcc gggcgttcgc gatccacgaa tttgccgacc acgatctctc
    24781 catcgaaggt caagtccact tcgctggtgg aggcgccgtg ccggactttg ctcttctcct
    24841 tgtagaattt catcgcgtcg agtccgtcgc ccattttgtt gcgccgctgg tacagcgtgg
    24901 ggcacggcga caatacttcg atgaaacaga atccctttcg gttgaatacc tccgccatgg
    24961 agcgcgtgag ctgccgcaca tgaaacgtgg tccagcgcgc cacgtaggag gctccggcgg
    25021 cttccgccag ggaaggcagg ttgaacgtag gctcgtaaga gccgtaaggc gcggtggtgc
    25081 tgatggccag ttgcggcgtg gtgggtgcgc tctgcccacc ggtcattgca taaatgaggt
    25141 tgttgacgca gatcactttg atgtcgatat tgcgccgcgc ggcgtgaatc aggtgattgc
    25201 cgccgatggc cgtcaggtcg ccgtcgcccg aataaaccac cacctgcaac tcggggttgg
    25261 caagcttcaa accggtggca aacggaatcg ctctgccgtg cgtggtgtgg aaagaatcga
    25321 gctcgacgta gcccgcgacg cggccggtac agccgattcc ggaaaccact gccactttgt
    25381 cgaggttggc accggattcc accaaagccc gggcgaaaca gttcaccgtc gtgccgatac
    25441 cgcagccggg acaccagatg tgaggcatgc gctcgctgcg caggaacggc tccacgggat
    25501 tgatttcaac ctgttcgaga acttcactca tcgcagagcc tccagaatgg cgtgcagaat
    25561 atcttccggg cgatggaccg cgcctccggc gtgagtcacc ggcactgtcc tggcttggcc
    25621 gcccgcggcg cgctccactt cgagcgaaat ctgacccagg ttcagttcgg gaacgacaaa
    25681 gaccctggtc cgggccgcga gttctttgat cttctgttcc ggaaaaggcc acgcggcaat
    25741 caaccggagc ttacccgctt taatgccctg ctgccgcgcc agttcgatgg cacgctgcgc
    25801 cacgcgcgcc gtgatcccgt aagagaccac tatcacgtcc gcgtcttccg tgccttcctc
    25861 ctcgaccagc gtcagacgct tcgacgcgtt ccggatcttg tccttcaacc ggcggaccaa
    25921 cttgtcctgc atctgcggat tcatggcggg ataaccacgt tcgtcgtgag tcaatccggt
    25981 gatgtgaaac ttgtagcctt cccctgcgtg aaccatttcc ggcacggcgt ccccattggt
    26041 tgcatacagg tggaattgac ccggtggctt ggtcgcgcgg cgccgcggcg tgatgcggat
    26101 ttcactggcg ggtggaatca ccactttttc cgtcatatgc cccacacact cgtccatcat
    26161 caccatcacc ggtacgcgaa attcctcaga gaggttaaat gcctcaatgg tgaggtcaaa
    26221 acactcctgg ggagaggagg ggcaaatagc gatgatctcg taatcgccgt gcgagcccca
    26281 gcgcgcctgc atcatatcgg cctgcgcggg gagagtgggg aggcctgttg acggcccgcc
    26341 gcgctggaca ttgacgaata cgcaaggagt ttccgtcatg acggcataac cgatgtgctc
    26401 catcatcagg gagaagcccg ggccggacgt cacggtaaaa gctttcgcgc cgccccaaac
    26461 ggcaccgaga atggtaatgg aagcagccaa ttcgtcttcc atctggatga acgttccgcc
    26521 gactgtcggt atgcggctgg cgaatctttc aacaatttca gtggagggag tgatgggata
    26581 ccctgcggca aatcgcgcgc ctgcggctaa tgccccttcc gtacacgcct gatcgccatc
    26641 caaaaaatgc gcgcccgtca gaacgccttt cgggtcagcg tgcatgaacc actccggtgc
    26701 cggcggcctg ccgaaacttg tagccgaaga tcgcaaagtc cgggcagtac atgccgcaca
    26761 ggtcgcaacc actacatttt tcaggagcta tcatatgggg cgggtgatac cccttggagt
    26821 tgaaagcaga agaaagcgag agtactttcg tcgggcagaa ctctacacag aagccgcaac
    26881 ccttgcagcg ttccaccaaa atagaaacgg atccttttgc cattgccacc tcagagagga
    26941 tgagtgcaat gggatgaaat gcacgcacgc tgccaagcgg acggcctcag tgcccgcggg
    27001 tggcgatggt tgtccagtct tcgggtgtcc ggccttccgg caggagggcg cctttatcca
    27061 gataccggat ctttgtggcg gagcgggcgg catggggatc gtgcgtcacc atcacgatgg
    27121 tcttgccgta ctcctggttc aggcgggaca gcagcgtcag cacttcctgg gcggaagagg
    27181 catcgaggtt gccggtaggt tcgtccgcga gaatgaggtc cggatcggtg acaatggccc
    27241 gggcgatcgc cacgcgctgt tcctggccgc cggaaagctg gcgcgggtag tgcgccattc
    27301 gatcacccag tcccaccagc ttcagcgccg tggctgcgtg ttccaggcgt tctttcttct
    27361 tgaggttcgt gagcttcaac ggcagctcca cgttttccat cgcggtcagc acggggatga
    27421 ggttgaagga ctggaacacg aacccgatat gcatgttgcg ccagcgggcg atttcacggt
    27481 cgctcaacgc tctcaggtcg cttcccaata ccaggatctg gcctccggtg ggccggtcca
    27541 tggccgcgat caggtgcagc agcgtggact ttccggagcc ggacggtccc atcaacgcga
    27601 cgaattcgcc tttggcaatg gagaggtcca cgtctttcaa cgcgaccacg tggaattcat
    27661 cgcgaatgaa ctccttggtg agttgccgtg tctcgatgat gtcatccgcc atgctcagcc
    27721 tttcctctct ttcacacggt cgccgtcttt caaatccgcg ggcggattga cgatcagatc
    27781 ctcaccgccg atcagccctt ccgcgatccg taccccctgc ggagtagttc cggcgggctt
    27841 gaccgtccgc cgcactgcct ttccatccag cacaacgaat actgcgttat cgcggacggc
    27901 ggagggtgga ataaccaccg ttcggaccgg cgctccgccc ggctccgctt tttcttccga
    27961 atagaaagcg acgctggcgt tcatttccgg acgcagatag tcgtcgggct ccaaaacttt
    28021 gaccttgatt tgcaccgtcg ctttctggcg gttggcttcg ggcgagatct cgtcgatgac
    28081 gccctcatac ttccggtccg gaaatgcatc ggttgtgacg atgccccgct ggcgcgggtc
    28141 cagtttcgca aaatcgttct ggctgatgtc cagttcgact tccaaatccc gcaggtccgc
    28201 gagcgaaacc acgtatcctt tggcgccccg ttcgccgacg aaactggtgg tcacaaattc
    28261 gcccttttcc accgcgcgtt ccagaatcgt gcccgtcacc ggtgctcgga tcacagtgtt
    28321 ctcgagttgc gtctcgccga atgcgaccgc gccgcgtgcc tgttccacct gccctcgcgc
    28381 ggcggcgatc tcttcctgcc gcgggccgat gcgcaccaac tcatacgcct tctccagcgc
    28441 gatcacacga gcggcccgcg cctcataagc ggcgcgcgca tcgtccagag tctgccgcgc
    28501 cagaactcct ccaccgacga gtttgttgct ccggtccagt gtgatcctgg cgttggcaag
    28561 gtccgcgcgc gcttcttcca cttccgcccg cgctcgctcg atctcttccg gacgtgaacc
    28621 gttcagcagc tcgctcaggc gggcttcgag agcggcaagc tgcccttttg cctgctgcaa
    28681 ctgcgcgcga tactcgtcgt cttcgagccg gacgatcacc tggccttcgc gaaccttgtc
    28741 gcccttgtcc acgccaatcc acgccacgcg cccgagtact ttcgaggcaa cctgaatctt
    28801 gtgcgccgcg acgatatagc cggtcgcatt cagcacaatc gtattggcgg cgttgccgcc
    28861 ggttgtttgc gcctgcaccc ggtggacttc cacttccgcc gcccgattca acaggctata
    28921 gccgtaaagc gcggcgcccg cgatcacgag cagagcgatg cccgcgataa tccagcgccg
    28981 ggaccaaccg gagccccggg gtgcttcttt cctggctctg tcgatacgaa gactcttgag
    29041 ttccgtttcc attggcatcc gggttcctca gttacccgct tgctgacgcg cgcggctccg
    29101 ttgaaaccat gtactcagat ctcccgaagc gctgtcagga tctctttgtt cgccgccatg
    29161 cgggccggaa aaacccctcc aatggctccc atcagcagcg cgaagagaag acccttcagc
    29221 atcagttccc ccgttacgcg aaagttgaac gcgatctcgc tgaatgtgac gaagcttccg
    29281 atgccggttt cgatgctgtt gagcggcagc accagcagac atcccaaaag acctcccacg
    29341 agcgacagta gaagcgattc gatcagaaag ctggacaaaa ttccagcttt cgaaaacccg
    29401 agcacgcgaa gcgtaccgat ttcggcggag cgccgtgcca ccgccgcata catcgtgttc
    29461 atcgccgcaa agctgctgcc aatcgccatg atgacggcca cgaacatccc catgaattcg
    29521 atgggtgcgg cggactgcat ctgctgttca tagtaagagc gctcggtctg cgccatgaca
    29581 ttgaagcggc ggtcgctctg aagatcgttg acgagggcct gcaccgctac ggggtccacc
    29641 gcacgcacca gggccgaact cagcacctcg ctccggttgt agtcggatgc gacctggttc
    29701 agatccgcga agatctcgct gttcgcggcg gagcggcccg cgtcgaacac gccgacgatc
    29761 tcccaatccc cacgcccgaa gcgtatgcgg ttgccgattt gagcggcact gtagcgattt
    29821 gcaatcgacc ggcccaccac cacctcgcgc cgtcccggtt ggaaccaccg tcccgcactc
    29881 agccgaatct cgtcgcgcat ctcgatgccg atcggcgcca ggccgcgcag agtgatgttg
    29941 ataccgtcgg gattgtccgg gctttcgaga gaaatcaccg tcaccatttc cagggacgcc
    30001 atcggctcgc cgtttttgga gcgcgcaatt cccggtttga agatcaggtc ctgaaatgtg
    30061 cttcgattga aattactggt caactcggaa gtggaccctt tccgcatcac cagaacatgc
    30121 aaaggatttc ccgtggcgcc cagggacgtt tgcaagcctt ccaccagcgc catgatggcg
    30181 agcagcacgg ccacggtcag ggcgattccc agggccgtca tcacggtggt ggtcttccgt
    30241 acggcgaggt tgcgaagatt gtacgccaat ggaatggcca cagtaccctc tcttaatccg
    30301 agaaccggag cgaatctaca atggaccggc gcgaggcgct ccaggcgggc agaaacgaac
    30361 tgaccgcgcc aatgaaacag gccagcgccg ctcctcctaa tatcaccgcg ggagggagcg
    30421 agagcgtttt gagatcgacc atcgtgatcg gcccctgtct gataaacgcg caaataactt
    30481 ccgaaagagc caacccgaga acgcctccga tcaatgcaat gaaaaccgcc tccgaaataa
    30541 tgatccccag aacggcgggc gatgtgaagc ccagcgtttt catgatgccg acctcccgca
    30601 cccgctcgcg caccgacatc gccatggtgt tgccggaaac cagcaacagc gtgaaggtga
    30661 tggcgccgca gatgcttagc aggaagagct tgacgttgcc cagatagccg aggaagctta
    30721 actggaaggc gcgttccggt tccgtcttgg tctgtgcggg ggcgttttgg aacatcgcat
    30781 caatgtcgtg ggcgatttgc ggcaccgagt ccggactgtc ggccagaatc gcaaacgtgc
    30841 tcactgcgtc gcggcgtgcc tccgggatgc tttcgcgcag gtattcatag tggaagaaaa
    30901 agttttcgtt atcgttgggg gcgtcgtaga cggcgcgaat ggtgaactcc atggtcaccg
    30961 ggaagatgtc gccgatcagc gtgacgcggt cgcccagttt ccagccgtag cgctccatca
    31021 gtgggcgtcc caccaggcag gcgctccgct ctcgccggaa ggcctctttc tggtcgtccg
    31081 gtatccggta ttccgggtac agcacgtaca acttttcggg ctcgacggcc attcgtgcga
    31141 agaaattttt gaaatcattg tccttgtaga tgccgccgaa ccactggatg atcgtgatgt
    31201 cccgaacacc gggcacctgc ttcatgcgat cgcggtagga taccggcatc acattggcaa
    31261 gcgaaatgcg attgcgggtg atcaggcgca gcgcctgctc cggggtagct tccccgaagt
    31321 agaaagcgtg gtacatcgcc atcagcactc cgagcaaaca cagcgatgcc gcgatgctca
    31381 gcaccgtcag aatgctgcga cgccggttgc gtaacacgtt tttaacaacc agggggaaat
    31441 accgggacat aaccgcttaa ttatagggta cgccttgcgg gagtagtttg ttccgtgagc
    31501 gcgccactaa gcgtcatcga gcacaaaggt ttcaaaggca gtgacccttt tgaagacagg
    31561 atcattggtg acgaatcctg tggcgcccga ccagacggca gtcgcggcgt gaagagcatc
    31621 cggtgtctta agccggtgct ctgcccggaa tcgagccgcc aggtccgcaa tctccagtcc
    31681 gggagggatc cattccagat ttggataagt cgtgagcagc ccatagaaga ggttgaccct
    31741 gtcttcatct ttctcacggt acggcggaac gagcaactcg gtcatggtga tcgtggatgt
    31801 gaccccggcg tgaccggact gctcgagcca ttggaagatc ccatccgcca gggtgacata
    31861 gcggccattt gcctccatct catagatgaa gatactggtg tcgatggcga tccggtgatg
    31921 acgccggagg aactggcgca acgatgtcag tcccagctcc gccgctcctt acggaggtgc
    31981 tgcggggaat agacacgctt cgctaaaccc cggagcgccg cctggtgaga agccggtttc
    32041 ggcagcatga ccacggtcga ccctcgcacg acgataagca ctttatcgcc cgctttcaga
    32101 gccaacgctt cccgcgcttc ccggggtatg acgatctggt ttttcgtgga aagagtcgcc
    32161 tcagtcatcc ctttacatta tcgcaaaacg taaagcagct cgcttcaaaa gtcacgaccg
    32221 cttgctcacg cgcgcggctc cgaaggcggt gcgagccacc ggatcgtccg gtacaacaat
    32281 aaccccgccg gtcccaggag gaaggtcaga accagcgcgg gcacgatgag cagatgggga
    32341 atgccgcgcc gttgtgcgtc gcgcgcctcc catccgccga tgaacagatc gaatgccagg
    32401 taatgggtcc acccggccag cagcgcccag ggattgtcga agagtgccct tacgcccgcc
    32461 agggaggaga acccaccctc gctccgcatc aggaccacag ccatcagcac tacgtagacg
    32521 gctgcgagca gcagcggcat caccaccggc acgatccttg aggcccagcg catccgcgga
    32581 agaaatacca gcggcagcca cgccgccacg gtcaccaggt tcaggatgga aaagagttgt
    32641 tcggctgtca tgatgcctcc ttcaaggcgc cggctgaggt ggcgtgtgag ccgcgaccga
    32701 tccagccaag gacgagcacc gttgtcacgg cccagatcgc catcgaggcg agcgccgtcg
    32761 catcgggcgc tacgacgctc tgcccccgca gcgcttgcca gagcagcagc agaaacagcg
    32821 ccgcatagct tgccgcggca gccagcagcg ccctgacccg aacggcctcg ggccggcgcc
    32881 atcgccgcaa gccggccccg atgagcgcca gcacctgaat cgaatgcaga ccgatgaaat
    32941 gcggtacgcg cagatcgccg tgttcccggc tccacccggt aaccggcaca ccgggaccgc
    33001 catccacgcc gctgacacta tgcgcgcccg cggtcgtcat ctgcccaccg gcacgggcat
    33061 gcgtaagctg cgccgccgtg gggcgggtca tcagcgggcc tgtcagcgcg ccggcaatcg
    33121 tcagcgtcat gccgagacgc agcgcccatc cgagtgtccg gtcggagaaa tggctccgcc
    33181 aaagcgcgac ggcgaccgcc acgctcacga acgtctgtat caaaatcgcc gctcccatca
    33241 cgaggaacag caccatattg agcgccgtcg atacattgaa atggctggtg gttccgcgcc
    33301 aggcctgggc gtcgatgatc gccacctcca gcacaaacac gatggcggtg gtccaaccca
    33361 caaggcggcg gacgcgcggc cagtccggca gccatgtgaa aatccacgcg agcgtgagac
    33421 tgtagatcgc ggtggagacc gcgaatttga agggtttgag ccaggcgggc gcgccggtaa
    33481 tcatgcgggg atcgacaagc atgcccagca gcgaaaaccc cgccacaacg gtcatgagcg
    33541 ctcccacggc ggtcagcggc gggctggtac gccacaagcg atccagcagg ccggcagctc
    33601 gaacccatgg gtgaaaccga acggtgcgtt gctttaaatc catgatcagc ccctctgatg
    33661 tggccagtgt caacatcaga ataacccaag atgttgacga tgtcaacact gttaatgcac
    33721 ggccgtgata tcttagcggt gctgaatgat tcccggaaca cgggggcgta tgaccagacg
    33781 ggaaatgata gcgatactgc cggcactgat ggtgccacgc gggaccggcg ccgcgccggc
    33841 gatagaatat gccggacttt ccggaccggt gggcgccgga gttggcgcca aaagagtgag
    33901 gaccttgcga atcgaagagc ccggagttta cgagaacatc ctcgtagacg gagagtgggc
    33961 cgaagaggac ctggtaagga tccggaccga cggtgtcgta ctccggaact gtacgatccg
    34021 ctacggccgg cgcgatgccc tcgaagttta tggccgcaat gtacgggtgg agaactgcca
    34081 cattcaccac gtgatcaacg gcacctaccg ggcggagaag aacctggatg cgcacgggat
    34141 tacgggccgc ccgctgaatc tcaccatacg gaataccgaa atttcacacg tcagcggcga
    34201 tgcgatccag ttcgatccgg ggcggcgagc cgagccgtac ccatgggata acgtcctggt
    34261 ggaccattgc tttctctgga ccagtccgct tgacaaggac tatgccggtt acaagcaagg
    34321 ggagcgtccc ggagaaaatg ctttcgacag caaaactcat gccggcgaac cgcggaagtc
    34381 ccgtatcacg tttcgcaatt gccttttgaa gggatgggga cacggagcca tcggaaacgg
    34441 agccgctctc aacctgaagg agaaagtgca ggcggtggtc gacaattgtg ttctggtcga
    34501 aaacgatatc gggttccggt gccgcggcac actgggcagc gcgtgggtga ctgcacgcaa
    34561 ctgcacggtc tatcggacct cgcgggtatt ccggctcgag catgaggtgc ggaacatcaa
    34621 aatcttccac ctcgctctcg gtgaaggcat tggacaactg tacgacaacg cgcccggtcc
    34681 gggcgaaggt ttcgaagagc acgggtcccg gaaggcacca cccctggcac agtggccgta
    34741 caaccggctg ccggtggggg aagtgatcgc gccgggcacg ccgccgtcct cggcggacga
    34801 tgccgttccg accgctctcg cgacgggttg gtcgtgagct agtcgaccag ggactggtac
    34861 accgtgagcg ggaacttctc gccgatgttg gttgcgttcg gcatcaactg gagcatgagc
    34921 acgaggacca tacggcttgc ggggtctact tcgtaagtgg ttccataggc tccgccccat
    34981 ccaaacgcgc caacgccggc catgcccttg gcgccgtaac ggtcgatggt ctggaatccc
    35041 agtccgaagc cgagcccgtc acctgaaggc agcgtgccgg attgattggt cgtcatcagc
    35101 gcgaccgtac gcggagcaag gatgcggact ccatccaact tgccgccgtt gcgaatcatc
    35161 tccagaaaac gagcatagtc acgtgcggtg gaggtgagcc cggcgccgcc cgcgaagctc
    35221 ttgcgcggac cgtccacgta ggagccctgg cccctcggtc cttcgggtgc acgaacaatg
    35281 agaccatcgc tgccgctcgc atatacggcg gccagacgat cgcgctccgc ggccggaagg
    35341 aagaagcgtg tgtccttcat tcccaagggc ccggtgatgc gggtctggat aaattgatcc
    35401 agcggcattc cggacgcctt ctccacgacg cagcccagaa tgtcggtgtt gtagccatag
    35461 acgaaggact caccgggctg ggcggcaaag ggaagggttc ccaaacgctc catggtgatg
    35521 cagatcggct cgtccttatc ggctgtgtac cagccgttgc cggcggcggg ccccagccct
    35581 ttctgcgcat acaatgcttc gatgtggctt tcccttccgt aggagatccc ggcggtatgg
    35641 gtcaacagtt gttgaatcgt gatggcgcgg cgcgcgggct cggtcttcat cccggagcca
    35701 tctttcaccg cgacggtggt cttcttgaac gtgggaataa aattccctac cggctcgttc
    35761 aaaccgatct tgccttcctc caccagagca aggatcgcgg cgctggtaat cgccttggtt
    35821 tgcgaggcga tacggaagat cgtgtccacg gtcatgcgcc gtcccgcctc ccggtcggcc
    35881 cagcccacag cgcgttcgta caccggctgg ccgtcacgga gcaccagggc aacggcgcct
    35941 gcaatccgct tctcgtccgt atagcgctga agcaactggc cgacgcgcgc cagccgttcc
    36001 tgcgcaagca ctgtccccgc ggatccggat aaggcggcgg aggaggctgc cgtctgggca
    36061 aacatcgcgc ccgccagcaa caccgtgatc aacgataaag agattccttt tttcattttg
    36121 gctctccacg atttgtacca cgggaacggg cgtatgatgg gctccatgtt cccgataagc
    36181 cgccgaattt ttctcggcgc actcgccgcg tccaggctac cggcacaatc gccgaggccg
    36241 aatgtggttc tcatcatcgc cgacgacctt ggctggggcg acatcgcgat caatggcgcg
    36301 ccggatatcc gtacaccgaa tatcgacggc atcgcgcgcg gtggcgtgcg attcacgcag
    36361 gcctatgcga acgctcccga atgcacgccg acgcggtgcg ccctgatgac cgggcgttac
    36421 cagcagaggg caggaggcct ggaatgcgcg atcggcgtca acaacatcgg ccgttacgac
    36481 gaggcagagt ggcttcagaa gcgcggcgaa ctgggcctgc cggcttctga acaaaccata
    36541 agcaaaatgt ttcaggccgc cggctatgac acggcgctca cgggcaagtg gcacctcggc
    36601 tacctggaga agcactggcc gagggagcac ggcttccaat attcgttcgg gattctaggc
    36661 ggcaatgccg actactacac gcacgaggaa cagggggagg gagccggtaa aacgcagatg
    36721 ttcgagaacg gctcgaaggt ccgccgccag ggatacatga cggacctgat cgcggacgcg
    36781 gccatcgggt ggctgaagca gcggacgtcg aagccgttct ttttatacct gccgttcact
    36841 gcgccgcact cgccgattca agcccccgag gatttcgatc cgcgcaccgg gacagcgccg
    36901 caccggccga agcatcgccc cacctacgcg aagatggtgg agcggctgga cacgcgcatc
    36961 ggcgacgtac tgcggcaact cgatgcgatg ggcgttgccc gcaacacgat tgtcgtgttt
    37021 gcctcggaca atggaggaga cgcgaacggc cgcaacgatc cgttcaaagg cagaaagagt
    37081 tccgtttttg aaggcggcat ccgcgcgccg cttcacatac ggtggcctgg tgtgattccg
    37141 gcgggccgcg caatcccgca ggttgcgatg acgatggacc tggcgccgac gctgttggga
    37201 gctgtgggcg ccggcggcag cgcccgcttc gatgggatcg acctgatgcc gtttctcaac
    37261 ggcacacaga ctccggtgag gcgcaccgtc ttctggcgtt ataaacgcgg gacgaatatt
    37321 cgcaaagcgg tccggcacgg ccgcttgaag tacgtggacg acaacggtgt gaaagcgctt
    37381 tacgatcttc gggtggactc caaagagacg accgatcttc tgaaaagccg gcccgatgcg
    37441 gtcaaggacc tcgaagaccg gctcgccgcg tgggagaagg acgtggaatc gccgcggtta
    37501 aaggagtttc ggaaaggcca tacgcaatgc tgaaaggtac ctggatgaag aacatcactt
    37561 ccgtcgctat tgccttgttc gtttccggcg cggcggcgtt cggcgccggc gacctgccgc
    37621 ttccacgttt cgccgatccg gcacgccggg cgaagctcga gaaggcgctc ccggaattgg
    37681 aaaaggtgtt cgaggaatac cgcctccggc ggggcattcc cgggctggtg tacggcgtgg
    37741 tgatcgacgg cgacctcgta tcggtgaaga ccatgggcgt tcgcgaccgg gaatcgaacg
    37801 caccggtcac gccggacacg gtgtttcgaa tcgcctccat gaccaagagc tttacggcgc
    37861 tcgccattct gaaattgcgc gatgagggga agctttcgct cgacgacccg gtttcgaagt
    37921 ggattccgca atttgcccgc ctggaatatc ccacgcgcga taccgctccg cttcgtgtgc
    37981 gccaactgct gacgcacagc gcaggattcc ctgaagacaa tccgtggggt gaccagcagc
    38041 ttggagtgag cgaggaccag cttaccgcct ggcttgaaaa aggactgcct ttttccactc
    38101 cgccgggcac ggcctacgag tactcgaact atggtttcgc tttgctcggg cgcattgtgt
    38161 cgaacgcctc gggaatgcct tatcgcgact acctggaaga gcaggttctt gcgccgctcg
    38221 gaatgacgca ttccacgctc gagccgtcca gcgtgcccgc tgatgtacga gcgacgggtt
    38281 atcgcaagac gggtgagacc tactcggtag aaaagccgct tgcacacggg ggattcggct
    38341 ccatgggcgg gcttctcaca tcagcacgcg atctcggacg ctatgtggcg tttcagttat
    38401 ccgcattccc accgcgcgat gaagaagatg gaggaccggt gaagcgaagc tcgcggcgcg
    38461 agatgcagca ggtgtggcgg caaggcacct tcaacgtgcg gcgatccgca ggcgattcgg
    38521 agttgcaggt ggaagccggc ggatacggct atgggctggc cgtctggcgc aactgccgct
    38581 tctcgcacgt cgtcgggcac ggaggaggat tgcccggttt cggatcgtat atgttgtggc
    38641 tgcccgaaca tggagtgggt atgttcgcca tggcaaacct gacatacgcc ggaccgcggc
    38701 agccgattga ggaatcgctc aacatcctgc gaaagaccgg cgcgctcaag ccccgcgagt
    38761 tacctgcttc gccggtgttg ctttcgacac gcgacgcgat cgtacgcctg tggcactcct
    38821 ggagtgagaa aggtgcggaa cagatggcgg cggacaatct gttcctggac agacccgctg
    38881 ccgagcggcg tgcggagatc gaacggatca aaggggctgt cggtgcatgc cgcccgccgg
    38941 aaaagataga cgccgaaaac tggctgcgcg gaaccttccg catgaactgc gagcgcggcg
    39001 ctgtggatgt attcttcact ctcgccccca cgaagccgcc tcgggtgcaa cacctgtcgt
    39061 tcgcgcgcac cagcgcgggc gtggcgcgtt ccacgcaaac gtccgcgtgc agcgaataaa
    39121 caaaacgtca tagataattg cgcatctcag caatctagaa aagcaaatta gcccaagttc
    39181 gtcacgaaaa ggatcaaaaa taggctaagc ccttcagaat cagtctagtg catcgtccaa
    39241 atagttcttt atagtataat gaatttctgg tacgctgggg tgtgtcccgc accgctcctg
    39301 taatttccct cgacccggag acgcgttcaa ccctgaatgg ttgggtgcaa gcctcctcca
    39361 cgccgcaagc tgtggcgatc cgcagtcgga tcgtgttggc tgcggccagc gggctctcca
    39421 accagcagat tgcgttgaaa ctcgatatcc ctgaagtcac ggtggggaaa tggcgccgat
    39481 cgtttgccga tcagggactg gagggactgc gagatgcggc ccgctcgggc cggccgccca
    39541 agcacgatgc ttcggtctgg caaagagtgc agacggcggc gtgtcaacag ccggagtccc
    39601 aaggacgctg gaccgttcgc acactggcgc gagaggtagg actgccacac agcaccgtcc
    39661 acgcgatctt gaacgcctcg gaactccaac tgcaccggat tcgaacgttc accttcagcc
    39721 ccgatcccga tttcgaggcc aaactactcg acattgtcgg cttgtatttg aatccacccg
    39781 agaatgcgct ggttctctgc gtggacgaga agcccggcat tcaggcgctg gaccgaacgc
    39841 aaccgttgct accactacgc gccaaacagc cccgcagttg gacca
//
LOCUS       DASTMK010000152         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_520075, whole genome shotgun sequence.
ACCESSION   DASTMK010000152 DASTMK010000000
VERSION     DASTMK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520075"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1463)
                     /locus_tag="VFQ79_06685"
                     /pseudo
     CDS             complement(280..1463)
                     /locus_tag="VFQ79_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611503.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1511..2332)
                     /locus_tag="VFQ79_06690"
     CDS             complement(1511..2332)
                     /locus_tag="VFQ79_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEU0139376.1"
                     /translation="MRWYILATLATMAFAADLKQDLTLHATFDGSTDAVFAGGDKRLY
                     TAPSYKEQASARPGIPDDVVLARGQGRSGDALHFRKKNTRAVFYEAERNIHFDFSNWS
                     GTISFWLNLDPETDLEPGFCDPIQVTDKAYNDSAIWVDFTKDEKPRHFRLGVFGNLKD
                     WNPGNTPANKNPAFNKRLIVVEQPPFARGKWTHVAIAFSGLGGGKGSARLYLDGKLRG
                     RTAAIEEPFQWDLSKGAIRLGVAYVGLMDDVAIFRRALTDEEITLLAKGAFWSEP"
     gene            2400..>3202
                     /locus_tag="VFQ79_06695"
     CDS             2400..>3202
                     /locus_tag="VFQ79_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139377.1"
                     /translation="MRETTYWERTDTFEEKLRLLEAAYRKNDYRLARAVADSIRQSIT
                     LEQQQQASLGEPVLKAAGSSSIAELPASWREWARGWSHYRILALDETVAVPRSREPVE
                     LIAAFPADQIASARREVRVARVDGGIPQEVPSQVTEEVRRGSQIHCRITFFADSPAHS
                     RTHWLVLHGNPDAELPDYPTDLRVTGEGFGLDLENEYYRAMLSKQMGQLERLVYKREH
                     GLELFAGGEGHGEPPGIDWAHDYVTSGNFQKLRITNWPSCPDYEVLRGPL"
BASE COUNT          665 a          976 c          911 g          650 t
ORIGIN      
        1 tctgaagtga acccctggag tgagacacaa agttaagggg gtaattaccg ttcaataaaa
       61 aatcgtggac ggcaaggttg cagttgctcc actctggaac tacctgagcg gaggataggg
      121 cactccaggg atggaacccg agcgtcgact cagcgaccgg aatggcaggg gcggcttcag
      181 ccgccctgtc gtgatacagc cattctggcg caactgcgtc aaatcccggg gccgcagggg
      241 cagagccccc gttgctggca cgcgttagat ggatgccgat catgatggag caccgttctc
      301 gaactcggcg ggcggaagat acccgagtgc cgaatgcagc cgtttctgat tgtagacgcg
      361 ctccaggaat tcaccaacgc tggcaagggc atcatgataa tcgcggtact cgttgcggta
      421 aacctcctct tgtttcaggg ttttcatgaa tgactcgcaa gccgcattgt catacgggtt
      481 tcccttacgg ctcatgctga tggtggcata atgttgcttc agcaactccg tgtaggcgtg
      541 cgccgcatac tgcacgccgc ggtccgagtg atgtaccaaa ccgcgagagg gttgccgatc
      601 gaccagcgcc atacgcaatg cggcgaccgt cagttcggct tccagggtgc ggcccagcgc
      661 ccagccgatc acgcgccgcg aaaatgcatc cagaaccaca gccaggtata cgaactcgga
      721 acgcaatcga atgtaggtga tgtcggcgac ccaaagctgg ttgatggctg ccggcgtgat
      781 ctcgcgggcc aagttcgggt aaaccggcag gttgtgccgc gagtcggtcg tgaccacgaa
      841 ggcacgccgc cgtacacaca gcagattgtc ttctcgcatc atccgcagca cgcgcttgtg
      901 gttgacctca aagccgcgtt gctgtagttc cgctgtgatt ctccgataac cgtacgcggg
      961 cgattccagc gcaaccgcct gtatttggtc gcgcagttcc atctccaccg gggtggcttg
     1021 gcgcggcagg cgccaacgat agaaaccggc acgggccaag ccggtcatct gacatagcgc
     1081 attcacggct tgacctttcg ccgaggcttg ctggatttct tcaaacaggc atccgcgcca
     1141 cttacgacgg ccggcggatg atgatccctg aaatgatgca atgccttttt taaaaaatca
     1201 ttctccaccg tcagttggcc gatcttccgc tcaagctccg cgatccggcg aacatcgtcg
     1261 agcgccggat caatgacacc acgtcgacca atgcccggaa atgccaactc tcccttggcc
     1321 cgccattctc cacgccaacg ttccagtagc ttcgggctca gttgatactt ccgcgcgatc
     1381 tcagcatgtg tcgatccagc gtccaaggcg tgcatggcgg cgatcttcag atcgcgactg
     1441 taaacagctc gggacaaatc cattcctcac tccattcaat gtatttgttc ccttaactcg
     1501 ttgtctcact ttagggttca ctccaaaacg cccctttcgc aagcagggta atctcctcat
     1561 cggttaacgc ccggcggaag atcgcaacgt cgtccatcag gcccacatag gcgacgccca
     1621 aacggatggc gcccttcgac aagtcccact ggaagggctc ttcgatcgcc gccgtgcggc
     1681 cccgcagctt accgtcgaga tacaaccgcg cagaaccctt ccctccgccg agcccgctga
     1741 aagcgatggc cacatgcgtc cattttccgc gcgcgaacgg cggctgctcg acgacgatca
     1801 ggcgcttatt gaatgccgga tttttgttgg ccggggtgtt gcccgggttc cagtccttca
     1861 ggttcccgaa gacaccgagg cggaaatgcc ttggtttctc gtcttttgtg aagtccaccc
     1921 agatggcaga gtcgttgtat gctttgtccg tcacctggat tggatcgcag aagcccggct
     1981 ccaggtctgt ttcaggatcg aggttcaacc agaaggagat cgttccggac caattcgaaa
     2041 aatcgaaatg gatgttgcgc tcggcctcgt aaaacacggc tcgcgtgttc ttcttccgga
     2101 agtgcaacgc atctcccgag cgtccctgcc ctcgcgcgag cacgacatcg tccggaatgc
     2161 cgggccgagc cgatgcctgt tctttgtaag aaggtgcggt atagagccgc ttgtccccac
     2221 ccgcgaaaac ggcatccgta gagccgtcga aggttgcgtg caaagtcaaa tcctgtttga
     2281 ggtccgcggc gaaagccatc gttgccagcg tagcgagaat gtaccagcgc attcgatcag
     2341 tttactcgag cgcgcagccc gcaattcatc cgtaataaga tgactaggca ctttcagcca
     2401 tgcgcgaaac tacctactgg gaacgaacag atacctttga agagaaattg cgcctgctcg
     2461 aagccgccta ccgcaaaaac gactaccggc tggcgcgggc cgtcgccgat tccatacggc
     2521 agtcgataac gctcgaacaa cagcagcagg cgtcacttgg agaaccggta ttaaaggcgg
     2581 ccggctcttc ctccatcgcc gaactccccg catcctggcg ggaatgggca cggggctggt
     2641 cacactacag gattctggcg ctcgacgaaa ccgtagcggt gccccgctcc cgcgaacccg
     2701 tcgagctgat cgcggccttt ccggcggacc agatcgcttc ggcccggcgc gaagtccggg
     2761 tggcgcgcgt cgacggaggc attccgcagg aagtcccgtc gcaagtgacc gaggaagtgc
     2821 gtcgcggctc tcaaattcac tgccgcatta ctttctttgc cgattcgccg gcacacagcc
     2881 ggacccattg gctggtgttg cacggcaacc cggatgccga gcttccggat tatcccaccg
     2941 atctgcgcgt gaccggcgag ggtttcggtc tcgatttgga gaatgaatac taccgcgcca
     3001 tgttgtcgaa gcagatggga caactggagc gcctcgtcta caagcgcgaa cacggcctgg
     3061 aattattcgc gggcggcgag gggcacggcg agccgccggg aatcgactgg gctcacgatt
     3121 acgtgacgtc cggcaatttc cagaaattgc ggattacgaa ctggccctcc tgccccgact
     3181 atgaggtcct gcgcggtcca ct
//
LOCUS       DASTMK010000153         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_551070, whole genome shotgun sequence.
ACCESSION   DASTMK010000153 DASTMK010000000
VERSION     DASTMK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551070"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1415
                     /locus_tag="VFQ79_06700"
     CDS             420..1415
                     /locus_tag="VFQ79_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEU0139378.1"
                     /translation="MKAAFIDKFGAPEGIRFDEFPTPVVGDSDVLVSVAAVTVNPIDT
                     YIRAGAFDAAVPLPFIIGRDMTGVVEAVGRHVTSFRRGDRVWANNQGYHGRQGTFAEF
                     CCVQEELLYRLPPEADLRDSVAVVHSGLTALLGLRLRAAINPGETIFINGGDGNVGSA
                     VLQFAKAAGARAIVTSAHHEKAAWSRELGADLVINYRKDDVVRTVLDFCPGGVDVYWD
                     ASATPDATRALEMTARRARLVFMAGLKHRTTLPVGAFYSKNLTLYGFTATDATTEELS
                     VCADEINRALQARTLRPRIAARLPLSSAAEAHHLIENTTQFGKIVLLPDPGEIAA"
     gene            complement(1504..1893)
                     /locus_tag="VFQ79_06705"
     CDS             complement(1504..1893)
                     /locus_tag="VFQ79_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0139379.1"
                     /translation="MKNKTLQFLLASVLVSALAAQQPKVTSLMSRELTETPGREGVVI
                     VVEYPPGGADPVHRHNAHGFIYVLEGSVVMQVKNGRKVTLTPGQTFYEDPGDVHVVGR
                     NASSTKPAKFLVFLVKDKGAPIFIPTR"
     gene            1963..>2143
                     /locus_tag="VFQ79_06710"
     CDS             1963..>2143
                     /locus_tag="VFQ79_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139380.1"
                     /translation="MRITSGQYSPKELNIGVVWAPWRRLEETPPRRWMVRGGCGHAME
                     ITPFGKWQCHTPTLSH"
BASE COUNT          438 a          655 c          603 g          447 t
ORIGIN      
        1 atccgcactc gttacttcca aatcttgcga tctgacgccc gctactacga gatcctgcag
       61 cgcatgaatc tcgacttgtc gcttccgagc catgctgatc ctcgctcaag agtttgtagc
      121 tttacctgat gcgtgctcag gaggccgaac cactgtggca gcacaaatct cgaacgacgg
      181 gcagccgacg ccggtcgacc ttaaatcgag accgcggttt attccccgga gcactcttaa
      241 tgggtggttg tgaaagttat aaagagcgat cccagaacat acgaacggag ccagcaccga
      301 attctgcacg gtgagtccgt ttttgactcc cccgggaccc gcgatactgc ctacgtcacg
      361 cggtcggcgg acacaccagc ggtatgttca ggcattcacc caggcaacga tcgaaaatca
      421 tgaaagcagc cttcatcgac aagttcggcg cacccgaagg gattcgtttc gacgagtttc
      481 caactcccgt cgttggcgat agtgacgtcc tggtcagtgt ggcggccgtc acggtaaacc
      541 cgatcgatac ctacattcgc gcgggcgctt tcgatgcggc ggttccgcta ccattcatca
      601 tcggccgaga catgaccggt gtagttgaag cggtcggccg gcatgtcaca agctttcggc
      661 gcggcgaccg cgtgtgggcg aacaatcagg gctaccacgg tcggcagggc acatttgcag
      721 agttttgctg cgtccaagaa gaactcctgt accggctgcc tccggaagcg gacctgcgcg
      781 attcggtggc agtcgtccac tcgggactga ccgcattgct cggcctgaga ctccgggccg
      841 ccatcaaccc aggcgagacg atcttcatca atggtgggga tggcaacgtg ggctccgccg
      901 tgctgcaatt cgcaaaagca gctggcgccc gcgcaatcgt cacctccgct catcacgaga
      961 aggctgcttg gtcacgcgaa ctcggagccg acctcgttat caattataga aaggacgatg
     1021 tcgttcgcac agtgctggac ttctgccccg gcggcgtcga cgtttattgg gatgcttcgg
     1081 caacgcccga cgcaacccgc gcgttggaaa tgaccgcccg acgagcgcgc ctggtgttca
     1141 tggctggact aaagcatcgc accaccttgc ccgtcggagc cttctacagc aagaatctga
     1201 ccctatacgg attcacggcc acagatgcca ccaccgagga actcagcgtg tgtgctgacg
     1261 agatcaatcg cgctctacag gcgcgaaccc tgcgccccag gattgcagcc cggcttcccc
     1321 tttccagcgc ggccgaagct catcatctaa tcgaaaacac gacgcagttc ggcaagattg
     1381 ttttgttacc cgatccaggg gagatcgccg cctgaccatt ggtgccgtat tcgccgacac
     1441 ctctcgagtc cgcggcgcgg gccgacgttc cggacgatcg gccctaagct gccagctggc
     1501 tcttcagcgt gtggggatga atatcggcgc acccttatct ttgacgagga acactaggaa
     1561 cttcgcgggc tttgtactgc ttgcattgcg cccaacgacg tgcacgtcgc cgggatcttc
     1621 gtagaaggtc tggcccggcg tcagggtaac tttcctgcca ttcttcacct gcatcaccac
     1681 cgatccctcc agcacgtaaa tgaagccgtg tgcgttgtgc cgatggacgg gatctgcccc
     1741 tccgggagga tactcaacaa cgataaccac accttctcta ccgggagttt ccgtgagttc
     1801 cctggacatg agcgaggtga ccttaggttg ttgagctgcc agcgcgctga ccagaacgct
     1861 tgcaaggagg aattgtagtg tcttgttctt catgacgagc ctttcactct ctctgctgca
     1921 atggcgattc catcacgctg ctccagcaat tgcgagtctg ccgtgcgaat aacttccggg
     1981 cagtacagtc caaaagagct caacatcggc gtcgtttggg ctccttggag aagactcgag
     2041 gagactccac cgcgccgctg gatggtgcgc ggcgggtgcg gccatgccat ggagatcacg
     2101 ccatttggta aatggcagtg ccatacgccc acgctgagtc acg
//
LOCUS       DASTMK010000154        19072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_167811, whole genome shotgun sequence.
ACCESSION   DASTMK010000154 DASTMK010000000
VERSION     DASTMK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19072
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167811"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VFQ79_06715"
     CDS             complement(<1..877)
                     /locus_tag="VFQ79_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0139381.1"
                     /translation="MLSLLWLVPAIPFATAAILALFGARMTRRAVAVVGAGSIGVSCA
                     ISLLIAPALLASEAPYQQTLWTWIDVAQFQPRIGFYLDAMSLLMMLVVTFVSFLIHLY
                     SAEFMEEDEGYSRFFAYMNLFVASMVVLILADNLLLLYLGWEGVGLCSYLLIGFWYRD
                     AANGLAARKAFIVTRVGDTAMAVGLFILFHSLGTLEIQPLMEQAQAQWAGGSPYATAA
                     ALLLLGGAVGKSAQLPLQTWLPDAMAGPTPTSALIHAATMVTAGVYLIARTNVLYTLA
                     ADAQLAVAVVGAVTLL"
     gene            complement(879..1184)
                     /gene="nuoK"
                     /locus_tag="VFQ79_06720"
     CDS             complement(879..1184)
                     /gene="nuoK"
                     /locus_tag="VFQ79_06720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU0139382.1"
                     /translation="MPILVRDGLLLAGTLFALGLIGLLVRRNVIFTLMSIEIMLNAAG
                     LAFVAAGARWGQADGQVMFLFVLAMAAAEVSVGLALVLRLYHHYRSLDTDAASRMRG"
     gene            complement(1102..1701)
                     /gene="nuoJ"
                     /locus_tag="VFQ79_06725"
     CDS             complement(1102..1701)
                     /gene="nuoJ"
                     /locus_tag="VFQ79_06725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU0139383.1"
                     /translation="MEIAFYIAGIVALISTMMTITRLNAIHALLYLVVSLLAVAVVFY
                     ILGAPFVAALEVIIYAGAIMVLFVFAVMLLNTGESDIVRERLLLSSEAWLGPSILSSV
                     LMAEVGWLLFQEPPTGAFPTAIEPKQVGIALFGPYLIGVELASLLLLAGLVGASHLGR
                     RDPGAGETEETENADTRARRPAAGGDLVRAGADRVTSAP"
     gene            complement(1709..2224)
                     /gene="nuoI"
                     /locus_tag="VFQ79_06730"
     CDS             complement(1709..2224)
                     /gene="nuoI"
                     /locus_tag="VFQ79_06730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU0139384.1"
                     /translation="MLSIVETIWRVFLHAFRKRVTVLYPEEKPYLAPRYRGRIILSCD
                     PDGGERCVACYLCAVVCPVDCIALQATEDEHGRRYPDFFRINFSRCIFCGFCEDACPT
                     YAIQLTPDFEMSEFNRQNMVYEKEDLLISGPGKYPGYNFWNVSGVAIGGKDKGQAENE
                     GRPVDVRSLLP"
     gene            complement(2239..3189)
                     /gene="nuoH"
                     /locus_tag="VFQ79_06735"
     CDS             complement(2239..3189)
                     /gene="nuoH"
                     /locus_tag="VFQ79_06735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU0139385.1"
                     /translation="MTSPYPIASILGILFASLTIAAGLIWLERRLLALWQDRYGPNRV
                     GPFGLAQVIADAVKIFFKQDWIAPFADKPVFIIAPAIILVTVLLSFAVVPFAPGIVVS
                     DPNIALLFFLGMSSLGAYSVVLAGWSSNNKYALLGSLRAAAQLLSYEVFMGLSVMGVV
                     MLAGSFQLSEIVHAQRGLWFVVPQFLGFVLFLIAGVAETRRLPFDLPEAESELVAGFH
                     SEYSGMKFGMFFVGEYLGVTLISAMITVLFFGGWLGPVLPPIVWFLLKMFLFISFFIL
                     LRASLPRPRFDQLMSWAWKLLLPLALLNLIVTGAVVLVLA"
     gene            complement(3186..5819)
                     /gene="nuoG"
                     /locus_tag="VFQ79_06740"
     CDS             complement(3186..5819)
                     /gene="nuoG"
                     /locus_tag="VFQ79_06740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEU0139386.1"
                     /translation="MAKVYVDNRAFEMNEKQNLLHGCLSLGFDLPYFCWHPAMGSVGA
                     CRQCAVKQYKDENDTKGSIVMACMTPASDGARISIRDPEAVEFRAAIIEGLMLNHPHD
                     CPVCDEGGECHLQDMTVMTGHDYRRYRFEKRTFRNQYLGPFVHHEMNRCIQCYRCVRF
                     YREYAGGEDLNVFSLRNTAFFGRESDGVLESEFAGNLVEVCPTGVFTDATLKRHYTRK
                     WDLQMAPSICVHCGLGCNVTAGERYGVLRRVVNRYHSGINGYFLCDRGRYGYEFVNSD
                     RRIRQPLLAGEPVTPEVARESLSAAVSAGRAIGIGSPRASLESNFALRTLVGEKNFYA
                     GIQDDQHRVLSLIVDVLRSRPTQVASLADVEASDAVLVLGEDLTNTAPRMALSLRQSV
                     RQKPNRVAEKLRIPLWLDHAVREAVQDDRGPLFIVTPAPTRLDNAATRTWRAAPDDIA
                     RLGFAVAHVLDPDAQDAPGLGESERALAGEIAAALRDAGRPLVVAGSSLNSTALIQAA
                     ANIAKALDAAGRRTGIVFTVPEPNSIGLALMNPKPLQEVFAQAAGTVIVLENDLYRRA
                     PAATVDEFLRQSENVIALDHLINRTVEQAELVLPAATYAEGDGTFVNNEFRAQRFFQV
                     LAPVDSIRDSWRWLATSWSVLDEVVAAISESMPELRRIREAAPSAAFRIAGEKIPRAP
                     ERYSGRTAMLANIDVSEPKPPEDPDSALAFSMEGTGKQPPPSLLPYFWSPGWNSIQSV
                     NKYQEEIAGPLRGGPAGVRLIEDGDGTLAKYFEPAPDAFRPREGEWLLVPLHHIFGSD
                     ELSRHAPAVRELSADAYLALSPADAAELEIAAGGELIVEGFRAPVKIVTGMARGMVGV
                     PAGLPPFDGLRLPMWSRLSKP"
     gene            complement(5841..7127)
                     /gene="nuoF"
                     /locus_tag="VFQ79_06745"
     CDS             complement(5841..7127)
                     /gene="nuoF"
                     /locus_tag="VFQ79_06745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU0139387.1"
                     /translation="METPLTQNISAGREPFGVREYEHAGGYQALRKALRDLTPAQVQE
                     EVKASNLRGRGGAGFPTGLKWSFSPVGDQAARPKYLVANADEMEPGTFKDRLLMEGDP
                     HQLIEGILLASYATEVDIAYIFIRGEYRTAAQRLSKAIAEAYESNYLGRNILGSGYSL
                     EMYVHSSAGRYMCGEETGMLNALEGKRANPRTKPPFPGVSGLFGKPTIVNNVETLCNV
                     PHIVNRGAAWFRNLGLTRDGGTKIFGASGKVKRPGLWELPMGTTVREILEEHAGGMRD
                     GVRFRGLLPGGASTDFLVEEHLDVRMDFTEPQKVGSRMGTGTMIVLDDKTCPVGMLWN
                     LEHFFAQESCGWCTPCWSGLAWVERILQAMEDGHGQPGDIEKLTFQTKFFGPGHTFCA
                     LAPGAAEPLQSGVKYFRDDFERHIREKRCPWAAGAV"
     gene            complement(7132..7596)
                     /gene="nuoE"
                     /locus_tag="VFQ79_06750"
     CDS             complement(7132..7596)
                     /gene="nuoE"
                     /locus_tag="VFQ79_06750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEU0139388.1"
                     /translation="MLSDEERREIEQEFPRYPTRQAVSIDAMKIVQKHRGWVSDEALH
                     DIAELLGMPASELDSVATFYNLIFRRPVGRHVIFLCDSVSCWIMDYERQRRHITEKLG
                     IGFGETTGDGRFTLLPIVCLGACDHAPAMMIDGDYHLDLDPQKIDTILEDYT"
     gene            complement(7607..9361)
                     /gene="nuoC"
                     /locus_tag="VFQ79_06755"
     CDS             complement(7607..9361)
                     /gene="nuoC"
                     /locus_tag="VFQ79_06755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HEU0139389.1"
                     /translation="MVASAPPMADVLRNRFEAGAVTAAPEARDSIPTFRVARERLREV
                     LGYLKTGVERPYTMLYDLTAIDERARTHRDGEPAGDFTVVYHLLSFERNEYLRIKTAL
                     DSGALSLDSITSLWPAANWYEREVWDMFGIVFSGHPHLERMLMPKTWVGHPLRKDHPA
                     RATEMGPFELPQEKEDAEQEALRFRPEAWGMQRTRAGADFIFLNLGPQHPGTHGVLRI
                     VLQLDGEEILDAVPEIGFHHRGAEKMGERQTWHTYIPYTDRVDYLGGVMNNLAYLTAL
                     EKLAGISVPPRAQVIRVLMSELFRIVSHLVWYGTFAQDLGQLSPVFYTFNDRERAFGI
                     VEAVCGARMHPNWFRFGGVAQDLPHGWEKMFRDFIEYFPPKLAEYDRTVLRNRIFRGR
                     TKGVGAYTASEAIEWGVTGPNLRACGFEWDFRKRQPYSGYEQFDFDIPTGQHGDCYDR
                     AVVRVEEMRQSLRIIGQCVDQMPEGPYKSDHPLATPPLKDRTMHDIETLITHFLGVSW
                     GPVVPPGEALGAIEATKGNNGYYLISDGGTTSYRTRIRTPSFPHLQMLPLICRGRTIP
                     DLLAILGAMDYVLADVDR"
     gene            complement(9361..9996)
                     /locus_tag="VFQ79_06760"
     CDS             complement(9361..9996)
                     /locus_tag="VFQ79_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU0139390.1"
                     /translation="MRWSLSKPEDNLRPAPGAGFEEEVKRSVILSRLQDLLAWSRKNS
                     IWPFNFGLSCCYVEMATSLTSKYDLARFGAEVIRGTPREADLIVIAGTVFLKMAPVIQ
                     RLYEQMMEPRWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVPGCPPRPDAFIEGV
                     TLLRDAVGTERRPLSWVVGPQGVERAPMPSMRDLKRGQRQLVTNLRPPEEV"
     gene            complement(9996..10367)
                     /gene="ndhC"
                     /locus_tag="VFQ79_06765"
     CDS             complement(9996..10367)
                     /gene="ndhC"
                     /locus_tag="VFQ79_06765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU0139391.1"
                     /translation="MMWPFVVYSLLVLLLAAGILAASHLLGERHRARATGSPYEGGIV
                     SEGSARVRLSAKFYLIAMFFVIFDLEAVFLFGWAIAARELGWAGFWEAFVFAGILVAA
                     LFYLARIGALDWVSPRGRTGN"
     gene            complement(10364..11761)
                     /locus_tag="VFQ79_06770"
     CDS             complement(10364..11761)
                     /locus_tag="VFQ79_06770"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEU0139392.1"
                     /translation="MTCVEQLATFVCRSVYEDLSSAAREQLKIRVLDSLGCAGGAVEG
                     DPIRILRRSIAGSGPEDRCTLIGGGSASPQNAAFYNGALVRYLDFNDSYVAPGETCHP
                     SDNLAPVLAAASYADRTGEELMTALAVAYQVQCRLSDVAPVRDKGFDDATLGAYSVAA
                     GLCRILDLDEERTANAIAICGAALNCLRVTRTGQLSHWKGLASPHTAACCTRIVFLAK
                     DGIGGPREVFEGDKGFMDAISGVFDIDWQREDLERVKRTILRRYNAETHAQAAIQGIL
                     DLRKRYRFEAPDVESVEVETFEVAFKLIGGGEEGDRTHCVTTKEQADHSLPYAIAAAI
                     LDGDVMPAQYAPERIAHPGIQELLRRVHVVANPAYSQRFPEEMPCRIQVLLRDGRTLA
                     KESRDYPGFVTQPMSWDAACSKFHSLMQPFAEEAERAAMIEAIAELESTSVRRLMRLF
                     AGVRPARNGREKEES"
     gene            complement(11891..12544)
                     /locus_tag="VFQ79_06775"
     CDS             complement(11891..12544)
                     /locus_tag="VFQ79_06775"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0139393.1"
                     /translation="MLAGDIYKLLEGTANAAFVVSLESEIRFWNRAAERLFGYPAAEV
                     LGKTCHSVLHGRGALGTFVCGAGCSVYSCAAGKMEVPNFDLEVVTKPGLSLWVSVSTI
                     VFVDTRMHERLVAHLAHDITGRKKIEEAFTHWLEFSKEALALGNGQPHLAPVTALSEQ
                     EKRILRLFAKAKNSGQVAKELGITLPTVRNHLSAINQKLRTHNRLEAVLHAMKRGLI"
     gene            12727..14064
                     /locus_tag="VFQ79_06780"
     CDS             12727..14064
                     /locus_tag="VFQ79_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0139394.1"
                     /translation="MQLVLLLIVIGLMHAARSFSPQSGLGTGPAGTTMAGGFLLLSAL
                     FAGNLSARLRMPQLTGYMIIGILVGPHVLALVTDQMLVQLRVFNGVAISLIALTAGVE
                     MDFQVMRPLMRGVAWVTGIAVVGTMVVLSAAAYLLRDLLPFMYGMSTVQLLAVSLVLG
                     VTLSAQSPAVVVALRKELDADGPLTRTVLGVVVLSDLVIVVLFALVSSLARSLLGGSA
                     ADSITTGILAWEIFGSALAGVFIGLLVTTYLRSIEGGGALFVITLGFLVAEVGQRIHL
                     DPLLVALFAGMFIRNLTSHGDRLHKEIEAASLPVYIGFFAVAGATIHLDALMTVGIPA
                     VILVLVRGLSFVAGSNAAARVAGSVEVVRKFTGFGLLSQAGLALVLALLFSRSFPQLG
                     AGASALVFGVVALNQIFAPILYRWALIRSGEAGSAHREPPVRVSAGGLVTENP"
     gene            complement(14077..14484)
                     /locus_tag="VFQ79_06785"
     CDS             complement(14077..14484)
                     /locus_tag="VFQ79_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU0139395.1"
                     /translation="MADQEHVELIKKDVDEWNRLREDDPTLSPQLAGADLRYLNLMFA
                     NLSRAVLRNADLALANLKGADLRWSDLRNANMVGAQMIGVDLMGADLSGADLRTAEDL
                     TTEQLAEARGDETTHLPDDLPRPPQWSSIAGAR"
     gene            complement(14593..15036)
                     /locus_tag="VFQ79_06790"
     CDS             complement(14593..15036)
                     /locus_tag="VFQ79_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139396.1"
                     /translation="MSVTEISLGNLRVAQMLPDVDLAPASGGSPVQLRPVRSETTVIL
                     LPHAFMCAACERYAMELDALAAEFRAWDARLLVITAAPDDWLQRLSFAKAAIHERDTA
                     SEAAVLVADRYGQVFHITRPGKEHAFPSPPEIVEWLKYLGTLCPE"
     gene            15086..15445
                     /locus_tag="VFQ79_06795"
     CDS             15086..15445
                     /locus_tag="VFQ79_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEU0139397.1"
                     /translation="MIYLHRVYDHETAPKGERYLVERLWPRGIRKEQLHVKEWLKEVA
                     PSTELRKWFSHDPAKWDAFKERYFAELDKHPDAWAPLEQAARKGAVVFFYSSKDTEHN
                     NAVALKAYIESKLKGTR"
     gene            15451..17655
                     /locus_tag="VFQ79_06800"
     CDS             15451..17655
                     /locus_tag="VFQ79_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139398.1"
                     /translation="MRHWNRRQLLRAAALFSAPLRSRGQDAPVAHPRALITPDMVREM
                     AEKVKGPFAAEYRMMLETASLGPQGLDNRWGIPAAFMEAGLAYQIERASGRDGQPFAE
                     KILDIWRDPEWQKPGLRRHFGWQGLLYDWIYDAMTDAERNRFGELLGEWVATWWNNGE
                     VNIPRSGWWYNQHWGPAHLDEPNHRVALLAKLFIDLAVMGHAGKFEDAVRRNLETFKA
                     RFLADGLPALDEMGGVWSESSGHGNYGPLLTVVLGYQAVKSGLGFDPFEKSAPHGFAR
                     EYVRAAIYGMMPHNDKLAYIDDSSSGYPEQQARAAPLFARHYGDRVARWLSDLALEKH
                     WLRSSRVPQDEVWQRIAFLPEKVQPQSPAEARWPLACLFRGAGHVYMRSAWNDANATW
                     AFFGAGPSYAGHSRDDEGHFLITRRGHVVNRSGGGGHNDNCYYAGGSLIYNIMTVYHP
                     DEQMRRAQYNENDGGLIRYVYSNDFPMERGHLTAFWHDDRLATYAAADLTKGYWAGKV
                     RQVTRQFLYLRGSHECFVIFDRVEATAAELPKVWMLHLPAEPEVDGTPRVVTPEHVTA
                     YEGSIARWWSEMAGDTDAISQGRTRAVLQTLAPRASRITKRGGEGHDFWGHPYNPAAQ
                     YNHTLDIKGIENKAYRRPPYSPWRLEVEPVESRARDYFLHVLFLKNEGDPDGARAERI
                     EQQNRLGARIPLGSRTATVLFDLEGLPGGRLTITDGGRTIHEADLTREIPRS"
     gene            17682..>19072
                     /locus_tag="VFQ79_06805"
     CDS             17682..>19072
                     /locus_tag="VFQ79_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0139399.1"
                     /translation="MSSDPAETQEVPLPAQPARPNAWVVLWRTIRRFDRAQIQPWLAL
                     RNSIGVGISLAAGFATGSVSSGLVAATGALNVSYTDGSDPYLPRVQRMLAANVLGTAA
                     VIAGPLCAPYPVFLLAVLVLWAAGAGILVALDAAAANIGIISLVTLVVYAAQPMPPEV
                     AIYSGLLAFAGGLLQTALSAALWPVRRYEPERRVLGALYSELSRVAASPVRAMSAPPA
                     SAQSTEAQEVLSGLWRSRTVQGERYISLLNQAERLRLALMMMGRLRIRLARHSEAQAA
                     ADMIESGLQMSSKLLASIGRSIESGVPLNPDPSWLSGVEDTGERLREFLSRDSSSALH
                     AMVDDARHQVDAAAGQLRAAIDLSASSTLPARAAFERRESHKPWRLRLAGTFAILRAN
                     VTPRSAAFQHGIRLSLCMALGEPISRALQLDRPYWVPMTIAIVLKPDFTATFSRGALR
                     VVGTYIGLIFATLL"
BASE COUNT         3783 a         5926 c         5716 g         3647 t
ORIGIN      
        1 ccagcagcgt tacggcgccc accacggcaa ccgcaagctg ggcatccgca gcgagagtat
       61 acagaacgtt cgtgcgcgcg atgaggtaga caccggcggt caccatggtg gctgcatgga
      121 tgagcgcgct ggtcggggtc ggaccggcca ttgcgtccgg caaccaggtc tgaagcggta
      181 gttgtgctga ttttccgacg gcgccgccta acagcaacag agccgcggcg gtagcatacg
      241 gcgaaccgcc tgcccattgt gcctgggcct gttccatcag cggttgaatt tcgagcgtcc
      301 ccaggctgtg aaacagaatg aacaggccaa cggccatggc cgtgtcaccc acacgcgtaa
      361 cgatgaatgc tttgcgggcg gcaagtccgt tcgctgcgtc cctgtaccag aagccgatca
      421 acaggtagct gcacaggccg actccttccc agcccagata cagcagaagc agattgtcag
      481 ccaggatcag gaccaccatg gatgcgacga acaggttcat gtatgcaaag aagcggctgt
      541 acccttcgtc ttcctccatg aactcggcgg aataaagatg gatgaggaag ctgacgaagg
      601 tgaccaccag catcatcagg agcgacatcg cgtccagata gaaaccgatc cgcggctgga
      661 actgtgcaac gtcaatccag gtccacagcg tttgctgata tggtgcttcg gaggcgagca
      721 gcgcgggtgc gatcagaaga gatatggcgc acgaaactcc gatggagccg gcgccaacca
      781 ccgcaaccgc acgccgcgtc attctcgcac cgaagagcgc aagaatcgcc gcggtggcga
      841 acgggatggc aggcaccagc cacagcagag acaacacgtc agcctctcat cctgctggcc
      901 gcatccgtgt caagggaacg atagtgatgg tacagccgca gcaccagggc cagcccaacg
      961 gaaacttcgg ccgccgccat ggccagaaca aataggaaca tgacctggcc gtccgcctgt
     1021 ccccaccgcg cgcccgccgc gacaaaggcc agcccggcgg cgttcagcat gatctcgatg
     1081 gacatcagag tgaagatcac gttacggcgc actagtaacc cgatcagccc cagcgcgaac
     1141 aaggtccccg ccagcagcag gccgtctcgc acgagtatcg gcattctccg tttcctccgt
     1201 ctctccggcg ccaggatcac gccggcccag atgactggct cctacgagcc ccgccagcag
     1261 cagcagggag gccagttcca ctccaatcag gtatggcccg aacaatgcaa tgccaacctg
     1321 tttcggttcg atcgccgtgg gaaatgcgcc cgtgggaggc tcctggaaca gcagccagcc
     1381 gacctcagcc atgagcacgc tcgaaagaat ggatggtccc agccaggctt cactgcttag
     1441 caacaggcgc tcccgcacaa tatcgctctc gccggtgtta agcagcatga cggcgaaaac
     1501 gaagagcacc ataatcgctc ccgcatagat gattacttcg agcgccgcta caaacggagc
     1561 gcccagaatg tagaaaacca ccgctacggc gagcagggac accaccagat acaggagcgc
     1621 gtgaatggca ttgagcctcg taatggtcat catcgtggag atgagggcca ctatccccgc
     1681 tatgtagaaa gcgatttcca tggcttgctc acggcaaaag ggaccgcacg tccacggggc
     1741 ggccttcgtt ttctgcctgt cctttatctt ttccgccgat cgccacaccg gacacgttcc
     1801 agaagttgta cccgggatac ttgcccggcc cgctgatcaa caggtcctct ttttcgtaga
     1861 ccatgttctg gcgattgaat tcactcatct cgaaatcggg cgtcaactga atggcataag
     1921 tcgggcacgc atcttcgcag aatccgcaaa aaatgcagcg ggaaaaattg atgcgaaaga
     1981 aatccgggta tcgccgcccg tgctcgtctt cggtagcctg tagcgcaatg caatccaccg
     2041 gacagacgac tgcacaaaga taacaggcga cgcaacgctc gccgccgtcc ggatcgcacg
     2101 acaaaatgat gcgtccacgg taacggggag cgaggtacgg cttctcttcc ggatatagaa
     2161 cggtcacccg cttgcgaaac gcatgcagga agacgcgcca gattgtttcc acgatgctaa
     2221 gcatccgaaa tcctccctct aagccagcac caacacaacc gctccggtca cgatcagatt
     2281 cagcagcgcc agcggcagca gcagtttcca ggcccacgac atgagctgat cgaatcgcgg
     2341 ccgcggcaac gatgcccgca atagaatgaa aaagctgatg aagagaaaca tcttcaacag
     2401 aaaccagaca atgggcggca gcaccgggcc cagccatcct ccaaaaaaga gaacggtaat
     2461 catcgccgag atcagcgtta cgcccaggta ttcgccgaca aagaacattc cgaacttcat
     2521 gccggagtat tcggagtgaa accccgccac aagctcgctt tccgcttcgg gaagatcgaa
     2581 gggcagccgc cgcgtttccg ctacgccggc gatcagaaac aggacgaacc ccagaaactg
     2641 cggcacgacg aaccagagtc cccgctgtgc gtgaacaatt tcgctcaact gaaacgaacc
     2701 ggccagcatc acgacgccca tcacggacag gcccatgaag acctcatagc tcaacaactg
     2761 cgccgcggcg cgcaaggaac caagcagcgc gtacttgttg ttcgacgacc aaccggccag
     2821 aactacgcta taggcgccca gcgacgacat ccccaagaaa aagagcagcg cgatattggg
     2881 gtcggatacg acgatacccg gcgcgaacgg caccactgca aacgacaaca gtaccgtgac
     2941 cagaataatc gcgggtgcga tgatgaacac cggcttatcg gcgaacggtg caatccagtc
     3001 ctgcttgaag aagatcttga ccgcatcggc gatgacctgc gcgagtccga agggtcccac
     3061 gcgattgggt ccgtagcggt cctgccatag agccagcagc cggcgctcca gccagatcaa
     3121 tcccgccgca atcgtaagcg acgcaaacag gatgccgagg atcgaggcaa ttggatacgg
     3181 gcttgtcatg gcttcgataa cctgctccac atggggaggc gcaacccgtc aaaaggcggt
     3241 agcccggccg gtacccctac catgccccgc gccattccgg taacgatctt taccggcgcc
     3301 cggaaacctt cgacgatcag ttcgccgccg gctgcaatct ccagttccgc cgcgtcagcc
     3361 gggctcaacg ccagatacgc atccgccgac aactctcgaa cagcgggcgc atgccggctc
     3421 aactcgtccg agccgaagat gtgatggagg ggaaccagca gccactcgcc ctcacgagga
     3481 cggaacgcat ccggtgccgg ttcgaaatac ttcgccaggg tgccatcgcc atcttcgatg
     3541 agccgtactc ccgcggggcc gccccgcagc ggacctgcaa tctcctcctg gtacttgttc
     3601 acggattgta tggaattcca tcccggcgac cagaagtacg gcagcaacga tggcggcggc
     3661 tgttttccgg tgccctccat gctgaacgca agcgcggaat ccggatcttc gggcggcttc
     3721 ggttcgctca cgtcgatgtt tgcgagcatc gcggtacggc cgctgtaacg ctccggcgcg
     3781 cgcggaattt tttcaccggc gatgcgaaac gcggcggatg gggcagcttc gcgaattcgc
     3841 cgcaactcgg gcatcgactc gctgatcgct gcgaccactt catccagcac gctccaggaa
     3901 gtggccagcc atcgccagga atcccggatg ctgtccaccg gcgccaacac ctgaaaaaaa
     3961 cgctgcgcgc gaaactcgtt gtttacgaac gtgccatcgc cttcggcgta cgtagccgca
     4021 ggtagaacaa gctccgcctg ttctactgtg cgattgatga ggtggtcgag cgcgatgaca
     4081 ttttcggact gtcgcaggaa ttcgtccacc gttgcggccg gggctcggcg gtacaaatcg
     4141 ttttcgagca cgatcaccgt gccggccgct tgtgcaaaga cttcctgtaa gggcttcggg
     4201 ttcatcagcg ccaggccgat actgtttggc tccgggacgg tgaacacgat gccggtgcgc
     4261 cggcccgccg catcgagggc tttcgcgatg ttcgcggctg cctgaatgag cgcggtgctg
     4321 tttaagctgg aacctgccac caccaacgga cgcccggcat cgcgcagcgc cgccgcgatt
     4381 tcgcccgcca gcgctcgctc gctctccccg agcccgggcg cgtcctgcgc atccggatcc
     4441 agtacatgcg ccacggcaaa gcccagccga gcaatatcgt ccggcgcagc ccgccacgtg
     4501 cgtgttgcgg cattgtcgag gcgcgtgggc gcgggtgtga cgatgaacag aggtcccctg
     4561 tcgtcctgca ccgcttcccg caccgcgtgg tccagccaca acggaatccg taatttttcg
     4621 gcaacccgat tgggcttctg gcgcaccgat tgccgcaggc tcagcgccat gcgtggagcg
     4681 gtgttcgtca gatcctcacc caatacgagc accgcgtccg acgcttccac atcggccagc
     4741 gacgccacct gcgtgggcct gctgcgcaga acgtcaacga taagcgagag gacgcgatgt
     4801 tgatcgtcct ggatgccggc atagaagttc ttctcgccaa ccagcgttcg cagcgcgaag
     4861 ttcgattcca acgacgcgcg cggggagccg atgccaatcg cgcggcccgc cgaaaccgcg
     4921 gcgctcaggc tttcgcgtgc aacctcaggc gtgacgggtt ctcccgccaa aagcggctgc
     4981 cgtatgcggc gatcgctgtt gacgaattca tagccgtagc ggccacggtc acagaggaag
     5041 tagccgttta tcccgctgtg atagcggttc acgacacgac gcagcacacc gtaacgctca
     5101 cccgcggtga cgttgcaccc caggccgcag tgcacgcaga tggagggcgc catctgcagg
     5161 tcccatttcc gcgtgtaatg gcgtttcaac gtggcgtctg tgaagacacc tgtcgggcac
     5221 acctccacca ggtttccggc gaactcactt tccagcacgc cgtcgctctc gcggccgaag
     5281 aatgcggtat tccgcagcga aaagacattc agatcttcgc cgcccgcata ctcacggtag
     5341 aaccggacgc aacgatagca ctggatgcag cggttcatct cgtgatggac gaaaggcccc
     5401 agatactgat tgcgaaacgt ccgcttctcg aaccggtagc gccggtaatc gtgcccggtc
     5461 attaccgtca tgtcctgaag atggcactct ccgccttcat cgcacaccgg acaatcgtgc
     5521 ggatgattca acatcaggcc ttcgatgatc gccgcgcgga actccaccgc ttccggatcg
     5581 cggatggaaa tgcgtgcacc atcgctggcc ggcgtcatgc aggccatcac gatgctgccc
     5641 ttggtgtcgt tttcgtcttt gtattgcttc accgcgcatt gccggcaagc gcccaccgaa
     5701 cccatcgccg gatgccagca aaagtaagga agatcgaaac cgagcgacaa acatccgtgg
     5761 agcagattct gcttctcgtt catttcgaat gcccggttgt ctacgtatac ctttgccatc
     5821 gtgcggctag ctccggctct tcacactgct cccgcggccc acgggcaccg cttctcgcga
     5881 atgtgccgct cgaaatcgtc gcggaaatat ttgaccccgc tctgcaacgg ttccgccgcg
     5941 ccgggcgcaa gcgcgcagaa cgtgtggccg ggaccaaaga atttcgtttg aaacgtgagt
     6001 ttttcgatat ctccgggctg cccgtgcccg tcttccatcg cctgaagaat tcgttccacc
     6061 caggcgagcc cgctccagca gggagtgcac cagccgcagg actcctgggc gaaaaaatgc
     6121 tccaggttcc ataacatccc gaccggacag gtcttgtcgt cgagcacgat catcgtgccg
     6181 gttcccatcc ggctgccaac tttctgcggc tcggtgaagt ccatgcggac atcgaggtgt
     6241 tcctccacga gaaagtccgt cgatgcaccg ccgggcagca gtccccggaa tcggacgccg
     6301 tcgcgcatgc cgcccgcgtg ttcctccaga atttcgcgaa ccgtggttcc catcggcaac
     6361 tcccacaggc ccggccgctt cacctttccg ctggcgccga aaatcttggt accgccgtcg
     6421 cgcgtcaggc cgagattccg gaaccaggcc gcgccgcggt tgacgatgtg cgggacgttg
     6481 cacaaggtct cgacgttgtt gacgatggtg ggttttccaa acaggccaga gacacccgga
     6541 aacggaggtt tggtccgcgg gttcgcgcgc tttccttcga gcgcgttcag catcccggtt
     6601 tcttcgccgc acatgtaacg gcctgcgctc gaatggacat acatttcgag gctgtagccg
     6661 gagccgagaa tgttcctgcc cagataattg ctttcgtagg cttcggcgat cgctttggaa
     6721 agacgctggg ccgcggttcg atattcgccg cgaatgaaaa tgtaggcgat atccacctcg
     6781 gtcgcatagc tcgccagcag tatgccttcg ataagctgat gcggatcgcc ttccatcaac
     6841 agacggtctt tgaacgtgcc gggctccatc tcgtcggcgt tggcgaccag gtacttggga
     6901 cgcgcagcct ggtcccccac gggagagaag ctccatttca gacccgtcgg aaaaccggcg
     6961 ccgccgcgac cgcgaaggtt tgaagccttc acctcctcct gcacctgggc gggggtcagg
     7021 tcgcgcagcg ccttgcgcag cgcctgatat ccgcctgcgt gctcatactc cctgacgccg
     7081 aacggctcgc gtcccgcgga gatgttttga gtaagcggcg tttccatggc gctacgtgta
     7141 gtcctcaaga atcgtgtcga tcttttgcgg atcgagatcg agatggtaat cgccgtcgat
     7201 catcatggcg ggcgcgtggt cgcacgctcc aagacagacg atggggagca gcgtgaaccg
     7261 tccatcgccc gtcgtttcac caaagccgat gccgagcttt tccgtaatgt gccgccgctg
     7321 ccgctcgtag tccatgatcc agcagctcac gctgtcgcaa agaaagatga catgtcttcc
     7381 cacgggccgg cgaaagatca ggttgtagaa agtggcaacg ctatccagtt ccgaggccgg
     7441 cattcccaac agttcggcga tgtcgtgcaa tgcctcatcg gagacccatc cacggtgctt
     7501 ctgaacgatc ttcatggcgt cgatcgagac ggcctgccgc gtggggtaac gtggaaactc
     7561 ctgttcaatc tccctgcgtt cctcgtccga tagcattcct tcctccttaa cggtcgacat
     7621 ccgccagcac gtagtccatg gcccccagaa tcgcgagcag atcgggaatg gttctcccgc
     7681 ggcagatcag cggaagcatc tgcaaatgcg gaaacgacgg cgtacggatg cgagtccggt
     7741 aggaagtggt tccgccgtcg ctgatcaggt agtagccgtt gttgcccttg gtcgcttcga
     7801 ttgcgcccaa agcttcgccc ggcggcacga ccggccccca actgacgccc agaaagtgcg
     7861 taatcagtgt ttcgatgtcg tgcatggtgc ggtctttgag cggcggcgtt gccaggggat
     7921 ggtcggactt atacgggcct tcgggcattt ggtcgacgca ctgcccgatg attcgcaggc
     7981 tctgccgcat ctcctccacc cgaaccaccg cgcgatcgta gcaatcgcca tgttgtcccg
     8041 tggggatatc gaagtcgaat tgttcgtaac ccgaataagg ctgccgcttc cggaaatccc
     8101 attcgaagcc gcacgcccgg agattggggc cggtgacgcc ccactcaatg gcctcgctgg
     8161 cggtgtaagc gccgacccct ttggtacgcc cgcgaaaaat gcggttgcgc aggacggtgc
     8221 ggtcgtattc ggccagcttt ggagggaagt actcaatgaa atcgcggaac atcttctccc
     8281 atccgtgagg cagatcctgt gcgacgccgc cgaaacgaaa ccagttggga tgcatacgtg
     8341 cgccgcagac cgcttcgaca attccgaaag cgcgctcgcg gtcgttgaac gtatagaaca
     8401 ccggcgagag ttgcccgaga tcctgcgcga aggttccata ccagaccagg tggctgacga
     8461 tgcggaacaa ctccgacatc aatacgcgga tcacctgtgc gcgaggcggg acagagatac
     8521 ccgcaagttt ttcgagggcc gtcagatacg ccaggttgtt catgacgccg ccgagatagt
     8581 cgaccctgtc tgtatacgga atgtaggtgt gccaggtttg ccgctcgccc atcttttcgg
     8641 cgccgcgatg atgaaatccg atttccggga cagcgtccag aatctcctct ccgtcgagtt
     8701 gcagcacgat gcgcaaaact ccgtgcgtgc cgggatgctg cgggccaaga ttcaggaaga
     8761 tgaaatccgc cccggctcgt gtccgctgca ttccccacgc ctcgggacgg aagcgaaggg
     8821 cctcctgctc ggcgtcctct ttttcctgcg gcaactcgaa aggccccatt tcggtagctc
     8881 gcgccggatg atcttttcga agcggatgcc ccacccaggt cttcggcatc agcatgcgct
     8941 ccaggtgcgg atgcccggaa aatacaatgc cgaacatgtc ccacacctcg cgttcatacc
     9001 agttggcagc aggccagagg gacgtgatgc tgtcgagcga gagcgcgccg gaatcgagcg
     9061 ccgtcttgat gcgcagatac tcgttgcgct cgaaggagag cagatgatag accaccgtga
     9121 agtcgccggc gggctcgccg tcccggtgcg tccgcgcgcg ctcgtcgatg gcggtaaggt
     9181 cgtaaagcat cgtgtaggga cgctccacgc cggttttgag gtatccgagc acttcccgca
     9241 gccgttcgcg agcaacccgg aatgtaggga tcgaatcgcg agcctccggc gcggcggtta
     9301 ccgctccggc ttcgaaccgg tttctcaaca cgtcggccat cggcggcgcg gaggcaacca
     9361 tcaaacctcc tccggcggac gcagattggt tacaagctgc cgctggccgc gtttcaggtc
     9421 gcgcatggac ggcatggggg cgcgctcaac accctgcggg cccaccaccc aacttagcgg
     9481 ccggcgttcg gttccaacgg catcgcgcag cagcgtgacc ccttcgataa acgcatcggg
     9541 ccggggcgga cagccgggca catacacatc gacgggaagg aatttatcca cgccttgcac
     9601 gacgctatag atgtcgtaca taccgccgga gttcgcgcag gagcccatag aaatcaccca
     9661 gcgcggctcc atcatctgtt catagagacg ctgaatgacc ggcgccatct tcaggaacac
     9721 cgttcccgct ataacaatca ggtctgcctc ccgtggagtg cctcggatca cttccgcgcc
     9781 gaagcgcgcc aggtcgtatt tgctggtaag gctggtggcc atttccacgt aacagcagga
     9841 aagaccgaag ttgaaaggcc agatggagtt cttgcggctc catgccagca ggtcctgaag
     9901 gcgggaaagg atgacactgc gcttcacttc ctcctcaaaa ccggcgcctg gtgccgggcg
     9961 caaattgtcc tccggcttgc ttaacgacca acgcatcaat ttcctgtcct tccccgcggc
    10021 gatacccagt ccagcgcccc gatgcgagcg agataaaaca acgcggccac caggattccg
    10081 gcgaagacga acgcctccca gaagcctgcc catccaagtt cgcgagcggc tatggcccag
    10141 ccgaagagga agaccgcctc cagatcgaaa atgacgaaga acatggcgat gagatagaac
    10201 ttggcggaca ggcggacgcg cgcggagccc tccgatacaa tgccgccctc gtaaggggag
    10261 ccggttgcgc gagcgcggtg ccgttcaccc aacaaatgcg aagcggcgag gatacccgcg
    10321 gccagcagca gcaccagcag cgagtaaaca acgaaaggcc acatcatgat tcctcctttt
    10381 cgcgcccgtt gcgtgccggg cggacaccgg cgaacagccg catcaggcgt cgcacactcg
    10441 tgctttcaag ctcggcaatg gcctcgatca tggcggcccg ctccgcttcc tccgcgaagg
    10501 gttgcatcaa cgagtggaac ttggagcagg ccgcatccca cgacatgggc tgagtgacga
    10561 aaccgggata atcgcggctt tctttggcga gagtgcggcc gtcgcgaagc agaacctgaa
    10621 tgcggcaggg catctcctcg ggaaagcgct gcgagtaagc gggattggca accacgtgaa
    10681 cccggcgcaa caactcctgg atgcctggat gggcgatgcg ttccggtgcg tattgggcgg
    10741 gcatgacatc gccgtccaaa atcgccgcgg cgatcgcgta aggcaggctg tggtccgcct
    10801 gctctttcgt ggtgacgcag tgtgtacggt ccccttcttc gccgccgccg atcagcttga
    10861 atgcgacttc gaacgtttca acctcgacgc tctccacgtc gggtgcttcg aaccggtagc
    10921 gtttgcgaag atccagaatt ccctgaatcg ccgcctgcgc gtgggtttcg gcgttgtacc
    10981 gccggaggat ggttcgtttc acgcgctcca ggtcctcccg ctgccagtcg atatcgaaca
    11041 caccggagat ggcatccatg aagcccttgt ctccttcgaa cacctcgcga gggccgccga
    11101 tgccgtcctt tgcgaggaac acgattcgcg tgcaacaggc ggcggtgtgt ggagacgcga
    11161 gtcccttcca gtgagagagc tgacctgttc tggtgacgcg caggcaatta agcgccgcgc
    11221 cgcaaatagc gatcgcgttg gcggttcgct cttcgtcgag atcgaggatt ctgcataagc
    11281 cggccgctac cgagtaggcc ccgagggtgg cgtcatcgaa ccccttgtca cgaaccggcg
    11341 cgacgtcgct cagccggcat tgaacctggt aggcaacggc taaagctgtc attaactctt
    11401 cgccggtgcg gtccgcgtag ctcgcggcgg cgaggaccgg cgccaggttg tcgctcgggt
    11461 ggcacgtttc tcccggcgcc acgtaggagt cgttgaaatc caggtaccgg accagcgcgc
    11521 cgttatagaa cgcggcgttc tgcggcgaag ccgagccacc gccaatcaat gtgcaccggt
    11581 cttccggtcc ggagccggcg atgcttcgcc ttagaatccg gatcgggtct ccttcgacgg
    11641 caccaccggc gcagccgagc gaatcgagga cccgtatctt caattgctcg cgagccgcgg
    11701 acgagagatc ctcatagacg gatcggcata caaacgtggc taattgctcc acacacgtca
    11761 tgcggcaatc cctgcgaatc cagactgcag cgcgccgcgc tgcaggctct gccttccaat
    11821 gtgaagcgga atgccggaag ttccaatagt ccgtttgtaa cgccggcagg gataaccgtc
    11881 acaagccagt tcagatcagc ccgcgtttca tggcgtggag tacggcctcc aagcggttgt
    11941 gcgtccggag tttctgattg atggccgaga gatgattacg aaccgtaggt aatgtgatgc
    12001 ccaattcctt ggcgacctgg ccggaattct tcgctttcgc aaacagcctg agaatgcgct
    12061 tttcctgttc cgagagcgcg gtgaccgggg caaggtgagg ctgtccgttg cctaacgcca
    12121 gcgcctcttt cgaaaattcg agccagtgcg tgaacgcctc ttcgattttt ttgcgcccgg
    12181 tgatgtcgtg ggccaggtgc gccaccagcc gctcatgcat tcgagtgtcg acaaacacga
    12241 tggtcgaaac gctcacccac agcgacaggc ccggcttggt taccacttcg agatcgaagt
    12301 tcggaacttc catcttcccg gccgcgcaac tatagacact acaaccggcg ccacaaacaa
    12361 atgtgcccag ggcgccccgc ccgtgaagga cgctgtggca ggttttgccg aggacctccg
    12421 cggccggata gccgaataat cgctcagcgg cgcggttcca gaaccggatc tcgctttcga
    12481 gcgacactac aaaggctgcg ttggccgttc cttcgagtaa tttatagatg tcgccagcca
    12541 gcatcgcaat ctccaaccgg atttcgctgc gctcggacgg acttcactcg gggggtggca
    12601 ccagagagtg ccgggaatgt cacggtatcg gatgccaccg ttccagtaag cgaattttag
    12661 cacgctctcc ggtgcgcgtt tctccgatgc cggaatgata gggtggattg tggagccgcg
    12721 tccggaatgc aactcgttct gttgctgatt gtcattggcc tgatgcacgc cgcacgttcg
    12781 ttttcgcccc agtccggcct cggcactggt ccggcgggaa cgaccatggc cggaggcttt
    12841 ctcctgttgt cggcgctgtt cgcagggaat ctctcggccc ggttgcgaat gccgcagctt
    12901 accgggtaca tgatcatcgg cattctggtg gggccgcatg ttctggcgct ggtgacggac
    12961 cagatgctgg tgcagttgcg agtcttcaac ggcgtggcga tctccctcat cgcgttaacg
    13021 gccggcgtgg agatggactt ccaggtcatg cggccgttga tgcgcggcgt cgcgtgggtt
    13081 accggcattg cagtcgtggg gacgatggtg gtgctttccg cggccgcgta tctactcaga
    13141 gatctgctgc ccttcatgta cgggatgagc acggtgcaat tgctggcagt gtcgctcgtg
    13201 ttgggggtca cgctgtccgc ccagtcgccc gccgtcgtgg tggcgttgcg gaaagagctg
    13261 gacgccgatg ggcctttgac ccggacggtg ttgggtgtgg tagtgctctc cgacctcgtc
    13321 attgtcgtgt tgttcgccct ggtatcctcg cttgcccggt cactgcttgg tggaagcgcg
    13381 gccgattcca tcaccacggg catcctggca tgggagatat tcggctcggc gctcgcaggc
    13441 gtgttcatcg gtctgctggt tactacctac ctgcgaagca ttgaaggcgg aggagcgctc
    13501 ttcgtaatta cgctcggctt tctggttgcc gaagtggggc agcgaattca tctggacccg
    13561 ttgctggtgg ctcttttcgc cggcatgttc attcgcaatc tgaccagcca tggagaccgc
    13621 ctgcacaagg agattgaagc ggcctctctt ccggtctata tcggattctt cgccgtggcc
    13681 ggggctacca ttcatttgga cgcattgatg accgtgggga ttcccgcggt aatcctggtg
    13741 ctggtaagag ggctttcgtt cgttgcgggc agcaatgccg cggcgcgcgt ggccggttca
    13801 gtggaagtgg tacggaagtt caccggattc gggctcctgt cccaggcggg tttggcgctg
    13861 gtgctggccc tgttattttc ccgcagcttt cctcaactgg gcgcgggggc ttcggcgctt
    13921 gtcttcggag tagtggcctt gaaccagatc tttgcgccta tcctctacag gtgggccctt
    13981 atccggagcg gtgaggcggg ctcagcccat agggaacccc cggtgcgtgt gtccgccggc
    14041 gggctcgtga cggaaaatcc ctgagtcgca gaaatttcag cgcgcaccgg cgatgcttga
    14101 ccattgcggc ggacgaggca ggtcatcagg cagatgcgtg gtttcgtcgc cgcgcgcttc
    14161 cgcaagctgc tccgtcgtca ggtcttcggc ggtgcggagg tcggctccgc tcagatccgc
    14221 gcccatcagg tccacaccga tcatctgcgc gcccaccatg ttcgcgtttc gaaggtccga
    14281 ccagcgaagg tcggctcctt tcagatttgc cagtgcgagg tccgcattcc gcaacaccgc
    14341 tcgcgaaagg tttgcaaaca tgaggttgag ataacgaagg tcggctccgg ccaactgggg
    14401 cgaaagcgtt gggtcgtctt cccggagacg gttccattcg tccacgtcct ttttgatcag
    14461 ctctacgtgt tcctggtcag ccattcgcga ttaccgaatt tccgttatag caaatagcgc
    14521 gaacccggcg ccaagccccg ctcactgtta ctcatcttcc cagtcgcccg gaccgggtat
    14581 atccggaacg tctcactccg ggcagagcgt gccaagatac ttcagccatt ccacgatttc
    14641 cggcggggaa gggaaggcgt gttccttgcc cggccgggtg atgtggaaga cctgtccgta
    14701 acggtcggcg acgaggacgg ccgcctctga tgccgtatcg cgttcgtgaa tcgccgcctt
    14761 ggcgaaggaa agccgctgca gccagtcgtc cggcgccgcc gtgataacca acaggcgggc
    14821 atcccaggcg cggaattcag cggcaagggc atccagctcc attgcatatc gttcgcacgc
    14881 cgcgcacatg aatgcgtggg gcaacagtat aaccgtcgtc tcgctgcgca ccggccggag
    14941 ttgcaccggc gaaccgccgc ttgccggcgc cagatccaca tcgggcagca tctgcgccac
    15001 ccggagattt cccagcgata tctccgtaac gctcataatt tcatcatgtc aggaatacag
    15061 gaataagata attacgagtt aggacatgat ctatcttcat cgagtctacg atcacgaaac
    15121 agcgccgaaa ggcgaacggt acctggtgga acgcctttgg ccgcgtggca tccggaaaga
    15181 gcaactgcac gtgaaagagt ggctgaaaga agttgctccc agcacggagc tccgaaagtg
    15241 gttctcacac gatccggcga aatgggatgc gttcaaagag cgctactttg cggaattgga
    15301 caagcatccg gacgcgtggg ctccgcttga acaggcggcg cgcaaaggcg cagtggtctt
    15361 tttctatagc tcgaaggata cggaacacaa caacgccgtg gcactaaagg cgtacatcga
    15421 atcgaaactg aagggaactc gatagacgtc atgagacatt ggaatcgccg ccaacttctg
    15481 cgggccgcag cgctcttctc cgcgccgttg cgctcacgcg ggcaggacgc gcctgtcgcg
    15541 catccgagag cgttgatcac tccggatatg gttcgggaaa tggccgagaa ggtgaaaggc
    15601 ccatttgccg cggaatatcg catgatgctc gaaaccgcga gtctgggtcc gcaggggctc
    15661 gacaaccgct ggggcattcc agccgcgttc atggaagcgg ggctggctta ccagatcgag
    15721 cgcgcctctg gccgcgacgg gcaaccgttc gccgagaaaa tactggacat ctggcgggat
    15781 cccgagtggc agaaaccggg gctgcgaagg cacttcggct ggcaagggct gctctacgac
    15841 tggatctacg acgcgatgac cgatgccgag cggaaccgtt tcggcgaact gcttggcgaa
    15901 tgggttgcca catggtggaa caacggcgag gtgaacatcc ctcgctccgg ctggtggtac
    15961 aaccagcact ggggcccggc tcacctggat gagcccaacc accgcgttgc actgctggca
    16021 aagctgttca ttgaccttgc cgtgatggga cacgcgggca aattcgagga tgccgtccgg
    16081 cgcaacctgg aaacgttcaa agctcgcttc ctggccgacg gtctaccggc cctggacgag
    16141 atgggcggcg tctggtccga atcgagcggc cacggcaact atggaccgtt attaacggtc
    16201 gttctgggct accaggcggt gaagagcggg ctgggtttcg atccgttcga aaaatccgca
    16261 ccgcatggat ttgcacgcga atatgtccgg gcggcgattt acggaatgat gccgcacaac
    16321 gacaagctgg cttacatcga cgattcctcc agcggctacc cggaacagca ggcgcgcgcc
    16381 gccccgctgt ttgcgcgcca ttacggcgac cgcgtagccc gctggcttag cgatctggct
    16441 ctcgagaagc attggctgcg ctcctcgcgc gtacctcagg acgaagtatg gcaacgcatc
    16501 gctttcctgc cggaaaaggt gcagccccag agcccggccg aggcgcgatg gccgctggct
    16561 tgtctgttcc gcggcgccgg ccacgtctat atgcgaagcg catggaatga cgcaaacgcc
    16621 acatgggcgt tcttcggcgc cggcccttct tatgccgggc actcgcgcga cgacgagggc
    16681 catttcctca ttacccggcg cgggcacgtc gtcaatcgct ccggcggcgg aggccataac
    16741 gacaactgct actatgcggg cggctcgctg atctacaaca tcatgaccgt ctatcatccg
    16801 gacgagcaga tgcgccgcgc gcaatacaac gaaaatgacg gagggctgat tcgctacgtt
    16861 tattcgaatg attttccgat ggagcgcggc cacctcaccg ccttttggca tgacgacagg
    16921 cttgctactt atgcagccgc ggacctgacg aaaggatact gggccgggaa ggtgcgccag
    16981 gttacgcgcc agttcctcta tctgcgcggc tctcacgagt gtttcgtgat tttcgatcga
    17041 gtggaagcca ccgctgccga attgccgaaa gtctggatgc tgcacctgcc cgccgagccg
    17101 gaagtggatg gtacgccgcg ggtcgtcaca ccggagcacg tcaccgcgta cgaaggaagc
    17161 attgcccggt ggtggagcga aatggcgggc gatacggacg cgatttcaca ggggcgtacc
    17221 cgtgccgtcc tgcagacgtt ggcgccgcgc gcttcccgta tcaccaagcg cggcggtgag
    17281 ggacacgact tctggggcca tccctacaat cccgccgcgc agtacaacca cacgctcgat
    17341 atcaaaggaa tcgagaacaa ggcgtaccgg cggcctccct actccccctg gcgtttggaa
    17401 gtggagcctg tcgaatcgcg agcgcgggat tatttcctgc acgtactgtt tctgaaaaac
    17461 gaaggggacc cggacggtgc gcgagcggag cgcatcgaac aacaaaaccg tctgggcgct
    17521 cgcataccgt tgggcagccg caccgcgacc gtgcttttcg atttggaagg gctgccgggc
    17581 ggacgcctca ccattaccga cggtgggcga acgatccacg aagcggacct gacgcgcgaa
    17641 ataccacgga gttaagtaag acactttgat gtgtgcgtag tatgtcttcg gatccagccg
    17701 agacgcagga agtcccgctg cccgcgcaac cggcccgtcc gaatgcctgg gttgtgctct
    17761 ggcgcaccat tcgccgcttc gaccgcgccc agatacagcc ctggctcgcg ctgcgaaatt
    17821 cgatcggtgt tgggatttca ttggcggcgg ggtttgcgac cggcagcgta tcgagcggcc
    17881 tggttgccgc caccggcgcg ctcaacgtaa gttatacgga cggcagcgac ccctatctgc
    17941 cgcgcgtgca acgaatgttg gcggctaacg tcctcggtac ggcggcggtg attgcgggcc
    18001 ctctgtgcgc gccctatcct gttttcctgc tggccgtgct ggtgctctgg gccgccggcg
    18061 ccggcatact ggttgcactg gacgccgcgg cggccaatat cggcatcatc agcctggtga
    18121 cgctggtcgt ttatgcggcg cagccgatgc ctcccgaagt agccatctac tcaggcttgc
    18181 tcgcgtttgc cggcggactg ctgcaaacgg ctctctccgc cgccctgtgg cctgtgcggc
    18241 gatatgagcc ggagcgccgt gtactgggag cgttatacag cgaactgtcg cgtgtcgccg
    18301 cctctcccgt gcgtgcaatg agcgctcctc ctgccagcgc gcaaagtacc gaagcgcagg
    18361 aagtgctgtc cgggttgtgg cgcagccgta cggtccaggg agagcgctac atctcgctct
    18421 tgaaccaggc tgagcggctg cggcttgccc tgatgatgat gggaaggctt cgcatccggc
    18481 ttgcgcgtca ctccgaagcc caggcagcgg cggacatgat tgagagcggg ctccagatga
    18541 gttcgaagct tctcgcctcc atcggccgtt ccatcgaaag cggagttccg ttgaatcccg
    18601 atccgtcgtg gctctccggc gtcgaagata ccggtgagcg gctgcgcgaa tttctgtcgc
    18661 gcgattcatc atcggccttg cacgccatgg tagacgatgc gcgccaccag gtggatgcgg
    18721 ctgccggaca acttcgcgcc gccatagacc tgagcgcctc ttccacgctt ccggcgaggg
    18781 ccgcgttcga acgccgcgag tcccataaac cgtggcgtct ccggttggcg ggcactttcg
    18841 ccatcctgcg ggccaatgtc acaccgcgct cggcggcttt tcaacacgga atacggctgt
    18901 ctttgtgcat ggcactggga gaaccgattt cccgggcgct ccaactcgac cgtccctatt
    18961 gggttcccat gacaatcgcc atcgtgctga agccggattt tacggccaca ttcagccgtg
    19021 gcgctctgcg tgtggtgggc acttatatag ggctcatttt tgccactctc ct
//
LOCUS       DASTMK010000155         5215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_231852, whole genome shotgun sequence.
ACCESSION   DASTMK010000155 DASTMK010000000
VERSION     DASTMK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231852"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFQ79_06810"
     CDS             complement(<1..753)
                     /locus_tag="VFQ79_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139400.1"
                     /translation="MRDRLIHRLTSFSVDHPWLVISVTLAITMAFGIQLSKMTTDTDP
                     KHMLPITSPVRQYNDQVEREFGLHADNLVVGIVNERGVVNSQTLSHIADLTRQIQEIP
                     GVVVRDVTSFPTIDNVAVEGGGLTVRPLLSEVPTSHKDLAALRMALLENPLLRNRVIS
                     DDTKATAIYVPLEPSANGREIAGRIREMLPKSNSGDQFFLAGDPIARDTFGYEMFQQM
                     GLFSPVAGMVMCLALWLMFRSWSVVIANMAVAM"
     gene            complement(760..1437)
                     /locus_tag="VFQ79_06815"
     CDS             complement(760..1437)
                     /locus_tag="VFQ79_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0139401.1"
                     /translation="MASRNRRFKTAIYEQFARIGKAISNPSRLELLDLLCQGPRTVEA
                     LAKEAGLGLANTSQHLKALREARLLEAEKAGLYVTYRLADEQVCQFFRSLRSLAETRL
                     AEVGEITRRFLEARQGLQPVDREQLLAKVRDGAVTVLDVRPPEEFRAGHLPGALSIPL
                     KELERRLADLPHDREIVAYCRGPYCVLAVEAVEMLRASGFVAFRLEDGVPDWQAKGLP
                     VAVGEET"
     gene            1504..2349
                     /locus_tag="VFQ79_06820"
     CDS             1504..2349
                     /locus_tag="VFQ79_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU0139402.1"
                     /translation="MQVVEFGIINSKVEMSNQQKVFPLFEHSLDAPSAFTPESLLEAV
                     RVERSLPQDPVPEICVLEFDGDLTDWLVATGQATKWRPWACFHTSMERVEVDGHPIGV
                     IARTIGGPYAVLVAEQLATSGARIILGLTSAGQVSPGLKIPSLVVPTKALRDEGTSYH
                     YLPAAETVDGDVDLATVLQDELSDLDSPVVTGTVWTTDAPYRETSEQLERHARNGILA
                     VEMQAASLFAFGAARGVRCGVVAHVTNGVDHSSEDQFDKGSHQLGFEILKAMSRAGRR
                     CLQDR"
     gene            2583..2786
                     /locus_tag="VFQ79_06825"
     CDS             2583..2786
                     /locus_tag="VFQ79_06825"
                     /inference="COORDINATES: protein motif:HMM:NF025810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein C"
                     /protein_id="HEU0139403.1"
                     /translation="MQIEPMCREFSYCSLRLADPDGRLTPEQVRDFYSSSYPEITTAS
                     IEGPEAVDGVLKFRFIRVLGTKG"
     gene            2783..2992
                     /locus_tag="VFQ79_06830"
     CDS             2783..2992
                     /locus_tag="VFQ79_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139404.1"
                     /translation="MTRRQAVAELEAFGQDPNRQQLQEKFKRCPESRKISELLGAAVL
                     GRKGYSGQIRREDSLPVPPSLVHPG"
     gene            3061..4005
                     /locus_tag="VFQ79_06835"
     CDS             3061..4005
                     /locus_tag="VFQ79_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139405.1"
                     /translation="MFTDGAQFAANLLRAGVAEAEDWNATRNIGAFLQRTVERFVGDR
                     AAKIDYAFDISFCLGTTASSWREPEEIDPRRILLTFRVAHTVGWVNLTPALDLLKAEH
                     ELLPTLFYHRLDDSLSRWFRVFNVQEARWRWESWNEMREEDEAERKEECEREGVPYEP
                     GERLDEPRLPECIGKMRRGKFPDVLALAHSPHAARLIRAAEHLNRASRRACCPRFDAQ
                     DREDLFPDSEAPVPLTALAFGEHDVITEFLNMELDSAGQVETEPWPILKMDGTDPRSI
                     RKAFRCASVALDTLEAASRTLSLVPGFEAMVKHNPYGV"
     gene            4008..4712
                     /locus_tag="VFQ79_06840"
     CDS             4008..4712
                     /locus_tag="VFQ79_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein B"
                     /protein_id="HEU0139406.1"
                     /translation="MYLDAEYGQSVPMNLVHAILLYGSEKMVRLATVHNPIKDPQGGP
                     PLLDAGEPLTRDFLDALVKGLGSELPAEILPGNVLVYSTSLTAWWEPAQVRSMFFAPD
                     CDGKTLNGKLFPHPPLVFGVHNGRLMVWALAEDRRPDADSWLYMAPYWNTYDDGSVCH
                     GSMPAPKTATIDNLPQWSHAFFASRFTGSNLGIQQCSHPEGFLGLWRSLIGKKKFPVE
                     HLLRKRRLREALCRNR"
     gene            4697..>5215
                     /locus_tag="VFQ79_06845"
     CDS             4697..>5215
                     /locus_tag="VFQ79_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system ThiF family protein"
                     /protein_id="HEU0139407.1"
                     /translation="MPKPLTAYRIKHWMLRDRITIVVVGCGGTGAALICGLPYLHHAL
                     LAAGHPSGIKACVIDGDRITETNCVRQPFTLSEVGLYKAQVLVNRLNVFWGLDWSAIP
                     THVRCGRDVPDCDFLISCVDTRAARATLAKVTRLKTRKFHLWIDTGNNADSGQFVMGE
                     PIRPGRKDRSTRL"
BASE COUNT         1047 a         1534 c         1607 g         1027 t
ORIGIN      
        1 catcgccacg gccatgttgg cgatgacaac cgaccagctc cggaacatga gccacagcgc
       61 gaggcacatg accatcccgg cgacagggga gaacagcccc atctgctgga acatctcgta
      121 gccgaaggtg tcgcgcgcaa ttgggtcgcc ggccaggaag aactgatcgc cggagttgct
      181 cttcggcagc atttctcgaa tccgcccggc aatctctctc ccgttggccg atggctcgag
      241 cggaacgtaa atcgcggtgg ctttcgtatc atccgagatc acgcggttcc gcaacagcgg
      301 gttctccagc aacgccattc gcaacgccgc gaggtctttg tgagaggtgg gaacctcgct
      361 cagtagagga cgcaccgtca ggccgccgcc ttccaccgcc acgttgtcaa tcgtggggaa
      421 gctggtcaca tcccggacca ccacgcccgg aatctcctga atctgccgcg ttaagtcggc
      481 gatatgggag agcgtctggc tgtttacaac accgcgctcg ttcacgatgc ccacgaccag
      541 gttgtcggcg tgaagcccga actcgcgctc cacctggtcg ttgtactgcc ggaccggaga
      601 ggtgatcggc agcatgtgct tgggatcagt gtcggtggtc atcttcgaca gttggatgcc
      661 gaaagccatg gtgatcgcca gggtgacgct gatcaccagc caggggtggt ccaccgagaa
      721 ggacgtgagt ctgtgaatga gacgatcgcg catgagcctc tacgtttctt ccccgacggc
      781 gacaggaagg cctttcgctt gccagtcggg gacgccatcc tcgagccgga aggccacgaa
      841 accgctggcc ctgagcatct cgacagcctc gaccgcgagc acgcagtagg gaccgcggca
      901 gtaggccaca atctcgcggt cgtgtggcag gtctgcaaga cgacgttcca attccttcag
      961 ggggatggac aaagcgcctg gcagatgccc ggcgcgaaac tcctcgggcg gccgcacgtc
     1021 gagcacggtg acggcgccat cccgcacttt ggccaggagc tgttcgcgat ccaccggctg
     1081 caaaccctgg cgagcttcca ggaagcggcg agtgatctcc ccgacctcgg ccaaccgggt
     1141 ctcggccaat gatcgaagag accggaagaa ctggcacact tgctcgtcgg ccaggcggta
     1201 ggtcacgtag aggccggcct tttccgcctc cagcagccga gcctcacgga gcgccttcag
     1261 atgttgagag gtgttggcca gacccagacc ggcttccttg gcgagggctt ccacggtcct
     1321 cgggccttgg cagagaaggt caagcagttc cagccggctg gggttcgaga tggccttgcc
     1381 gatgcgagcg aactgctcgt agatggcggt tttgaaacgg cggtttcggc ttgccatgat
     1441 tccttcagta atctataatg tactgaacta ctataacaac gtcaagagtc ttgtgcgact
     1501 ctattgcaag tcgttgaatt tgggattatt aatagcaagg tagaaatgtc gaaccagcag
     1561 aaggtctttc ccctgttcga acactcccta gacgcaccga gcgcgttcac cccggaatcg
     1621 ctgctcgagg cagtgcgtgt cgagcggagc ctgccgcagg atcccgtgcc cgaaatttgc
     1681 gttctggaat tcgatggcga cttgacggac tggctggttg cgaccgggca ggccaccaag
     1741 tggcgtccat gggcctgctt ccacacgtct atggaaaggg tcgaagttga cggccatccg
     1801 atcggcgtga tcgcacggac gataggcggc ccgtacgcag tgttggtggc tgagcagctt
     1861 gcgacctccg gcgcccgcat cattcttggg ctgacgtcag cgggccaagt gtcgcccggc
     1921 ttgaagatac cgagcctggt ggttccgacg aaggcccttc gagatgaagg aacgtcgtac
     1981 cactacctgc cggcagcgga gaccgttgat ggcgatgtcg accttgcgac agtcttacaa
     2041 gatgagctct ccgatctcga ttcgccagtc gtcactggca ccgtatggac gactgacgca
     2101 ccgtaccgcg agacctctga gcagttggag cggcacgcga gaaacggcat ccttgccgtg
     2161 gagatgcagg ccgcgtccct cttcgccttt ggcgcagcac gcggagtccg atgcggcgtg
     2221 gtcgcccacg tcaccaacgg cgttgaccac tcgtcggaag atcagttcga caaaggctcc
     2281 caccagcttg ggttcgagat tctcaaggcg atgtctcgcg ctggcaggcg ctgcttgcag
     2341 gatcgctgat cgccgagtgc tgcacccgtc gaagcgacgg cgaacatgac ccctcactca
     2401 gcgggcaaga cgtagagctt gattcttgca gcgtgacttg tacagtaact ccggcccggc
     2461 tctatgtcat caaattggac atcttgtgtc attctggtgg accccattag tcacttctac
     2521 gtcagaactt gtcagtaatt acgtcaacct acaaaaacaa caacaacaac aaggagaaat
     2581 ctatgcagat cgaacctatg tgccgtgagt tttcctattg cagtttgcgg cttgcggatc
     2641 cggacggacg gctcacaccc gagcaagtca gagatttcta cagcagttct tatccagaga
     2701 tcacgactgc atcgatcgaa gggccggaag ccgttgacgg tgttctgaag ttcaggttca
     2761 tccgggttct gggaactaag ggatgacgcg cagacaggct gtggccgaac tcgaggcgtt
     2821 tggtcaagat ccgaatcgac aacaacttca ggagaagttc aagcgatgcc cggaatcccg
     2881 caaaatatcc gaattgctgg gcgcagcagt gctgggccga aagggttatt cggggcagat
     2941 tcgccgcgaa gactcgctgc ccgtgccgcc aagcctggta cacccgggct aatcgctcct
     3001 gggccggggc tgtgcctacc acgtctgcag gagcaccatt ggcagttctc gcgcaacgct
     3061 gtgtttaccg atggcgctca gtttgccgcc aacctgttgc gggccggagt ggccgaagcc
     3121 gaggactgga atgcgacacg caacatcggg gcgtttctcc aacggacagt tgaacgcttt
     3181 gtcggcgatc gtgcggccaa gatcgactat gccttcgaca tcagtttttg cttaggcacg
     3241 accgcttcca gttggcgcga gccagaggag atcgaccccc ggagaatcct gctcacgttc
     3301 cgtgtggcgc atactgtcgg ctgggtcaat ctcacgccgg cgctcgatct gctcaaagcc
     3361 gagcacgaac tgctgccgac actcttctat caccggctgg acgattcgct gtcccgctgg
     3421 tttcgcgtct tcaatgttca ggaagcccga tggcgctggg aatcgtggaa cgagatgcga
     3481 gaagaggacg aggccgaacg caaagaggaa tgcgagcgcg aaggcgttcc gtatgagcca
     3541 ggcgaacggc tcgatgagcc aaggctgccc gagtgcattg ggaagatgcg tcgtggcaaa
     3601 tttcctgacg tcctggctct ggcgcacagt ccacatgcgg ctaggctgat tcgggcagcc
     3661 gagcacctga accgggcttc gcgacgtgcg tgctgtccaa gattcgatgc tcaggaccga
     3721 gaggatctgt tcccggacag cgaggcgccg gtccctctga cggcactcgc gttcggcgag
     3781 catgacgtga ttaccgaatt tctgaatatg gagctcgaca gcgccggcca ggtcgagacc
     3841 gagccgtggc cgattctcaa gatggacggc acggatccac gatctatccg caaggcgttt
     3901 cggtgcgcca gcgtcgctct ggacacgctt gaggccgcct cccgcaccct gtcgctggta
     3961 ccgggttttg aggccatggt caagcacaat ccctacggag tttgagaatg taccttgatg
     4021 ccgaatacgg ccagtccgtg ccgatgaatc tggttcacgc gattctgttg tacggcagcg
     4081 aaaagatggt ccgcctcgcc accgtacaca atccgatcaa ggacccgcag ggcggaccac
     4141 cgctgctcga tgccggcgag ccactcacac gggacttcct tgacgccttg gtgaagggtc
     4201 tcgggagcga gcttccagcc gagatccttc caggcaacgt tctcgtttat tcgacttcgc
     4261 tcacggcgtg gtgggagccg gcgcaggtcc gcagcatgtt ctttgcgccc gattgcgacg
     4321 gcaagacttt gaatggaaag ctgttcccac atccaccgct cgtgtttggc gtgcacaacg
     4381 gtcggttgat ggtctgggcc cttgctgagg accgtaggcc agacgcggac agctggctat
     4441 acatggcgcc gtattggaac acgtacgacg acggaagcgt ctgccatggc tcgatgccgg
     4501 cgccgaaaac agcaacgatc gacaatctgc cgcaatggtc gcatgcattc ttcgccagcc
     4561 ggtttaccgg ctcgaatctg ggcattcagc agtgtagtca cccggagggc ttcctcgggt
     4621 tgtggcggtc gttgatcgga aagaagaagt tcccggtcga gcatctgctc cggaagcgga
     4681 ggctcaggga ggcgctatgc cgaaaccgtt gacggcgtat cgaatcaagc actggatgct
     4741 gcgcgatcgc atcacgatcg tggttgtagg ctgcggcgga accggggccg ctctgatctg
     4801 cggcctgcct tatcttcatc atgcgttgct ggcagctggc catccctccg gaatcaaggc
     4861 ttgcgtgata gatggggacc gcatcacgga aacgaactgc gtgcggcagc cgttcacgct
     4921 cagcgaggtg gggctgtaca aagcgcaggt gctggtcaat cggttgaacg tgttctgggg
     4981 tctcgattgg tccgctattc cgacccacgt gcggtgcggg cgtgatgtgc ccgactgcga
     5041 cttcctgatc agctgcgtgg atacacgggc agcaagggcc acgctggcca aggtgacccg
     5101 gctgaagaca cgcaagttcc acctctggat cgataccggc aacaatgccg actccgggca
     5161 gtttgtcatg ggagagccaa tacggccggg ccggaaagac cgctcaacac gcctg
//
LOCUS       DASTMK010000156        14708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_296279, whole genome shotgun sequence.
ACCESSION   DASTMK010000156 DASTMK010000000
VERSION     DASTMK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296279"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFQ79_06850"
     CDS             complement(<1..1283)
                     /locus_tag="VFQ79_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0139408.1"
                     /translation="MFWLIVFFACSLHTPYAAAFQAPPAPEAEQSKPAAASESQPDTT
                     IDFAAEGMKALESEKYERAVELFSKAAEADSSDYAAHFHLGFAYSMLQRDSEAISEYR
                     KVLELKPGLYQAQLNLGILLLRNKRPKEALPVLEAAGAQKPGEFRPVFYLAEAQFQSA
                     DFAKAAESYGKALELDAKSAPAALGMARSFVKLGRPAEAIPHYRKAAELDPSYRDTLL
                     ELATVLESAQQREEAIALYRQFPDHAAAQERLGELLIETGKPGEAISPLEFAVKQSPT
                     AANRLALATAYLRSNETDKALGMLKEALRMEPANTGLRLHYGRVLRDQKNYAAAAEQF
                     EAVARAEPRSVEVWNEVAAARILLEKYDRALEALDKLKSLGAETASHYYLRALALDHM
                     KVYKPALENYQAFLAMSRNQHPDEEFKARERIKRI"
     gene            complement(1324..2130)
                     /locus_tag="VFQ79_06855"
     CDS             complement(1324..2130)
                     /locus_tag="VFQ79_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU0139409.1"
                     /translation="MSRMARRLPALILLLTCLAPAQTDMAPFFKKGAIRALIFSGRNN
                     HDWRTTTPFLRELLLATRRFDVRVTEEPAGITARTLAPYDVVVLDYNGPRWGETAEKA
                     LVEFVRSGKGLVAVHAASYAFGGLDTLGDRHVRTGIIEPPWPEYAEMTGATWSEQAPK
                     TGHGKRHSFRVKFVDPDHPIAKGLGPDFLANDELYHSLRMRPGVKVLATAYDSPDIGG
                     TGKDEPILWTVDYGSGRVFHTALGHDLDAMREKGFVATFTRGTEWAAGHR"
     gene            complement(2127..3314)
                     /locus_tag="VFQ79_06860"
     CDS             complement(2127..3314)
                     /locus_tag="VFQ79_06860"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139410.1"
                     /translation="MIVSSISRRAFVGSAVPAASYSRVLGANDRVRLGIIGTGNRGSY
                     LMRVANKVGGIEWVAVSDAWDRRRDEAEKTAGVPVKKDADYRQLLDRTDVDAVIVATF
                     DHLHTPISVAACQAGKDVYVEKPMTSIPEQGRELVKAVRSAKRVLQVGVQQRSMAVFQ
                     EAKQRFFDTGLIGRVHMVRTIWNANGGYLTPVPPDMDRKPAGLNWDACLGWLPKRPWD
                     PKRYFNRFAYWDFSTGGQTGGLFVHMVDVVHWYLGLTRPDSAVALGGIYQHDDGRDTP
                     DNINLILNYPERLNVTFEASISDMVAKEAASIVFMGTGGRLSIFRGGYRFIPRERNAP
                     EITAPGSPEEAHMANWLDCVRSRKEPNANVVDGHYGSMACHIGNIAYQKKSRIEWRPE
                     WNV"
     gene            3465..4148
                     /locus_tag="VFQ79_06865"
     CDS             3465..4148
                     /locus_tag="VFQ79_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0139411.1"
                     /translation="MPVASQAEFKHANRAQLSLFAPLEKRVLVWMAQRMPAWVSSDHL
                     TFLGFAAMLLAGLSYWAARWNSYALLLVVVWLAVNWFGDSLDGTLARVRDRQRPRYGF
                     YVDHITDAFGTFFLLGGLALSGYMSPLIALGLLIAYFLLSIEVYLATYTIGTFHLSFW
                     KFSPTELRVLLAIGNIALLFRPVVELFGHKYLLFDIGGVCGIVGMAVMVVTAAVRHTR
                     TLYNAERLP"
     gene            4148..4624
                     /locus_tag="VFQ79_06870"
     CDS             4148..4624
                     /locus_tag="VFQ79_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU0139412.1"
                     /translation="MLLVRWLKFNAVGGLGIGVQLGVLALLAGACSVNYLAATIIAVE
                     AAVLHNFTWHERYTWADRTSAPGGFWLRLVRFHTSNGLISIAGNALLMRLLVGGLRWN
                     YLIANLVAIAACGLLNFAAAEWFVFRRPVKLFAHLPIASMWKEDANPDWRRVKPPC"
     gene            4573..4959
                     /locus_tag="VFQ79_06875"
     CDS             4573..4959
                     /locus_tag="VFQ79_06875"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0139413.1"
                     /translation="MERRREPRLAASQAALLTTLTEPCLKRNGTIVNVSGRGINVSLE
                     QPLPVDVPVRIDLDDTLALGEVCYCEPVEGRYSVGIQVDQVLNGLESLLRLNEVLLGE
                     TPRKSSGENSPEAAHALKDGHHQNQK"
     gene            5180..6061
                     /gene="rfbD"
                     /locus_tag="VFQ79_06880"
     CDS             5180..6061
                     /gene="rfbD"
                     /locus_tag="VFQ79_06880"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU0139414.1"
                     /translation="MAKRVVIFGSAGQLGVELVREFSQRNYSVMGYDRATVDIADSSA
                     VEREIAAADPELVVNAAAYNQVDLAEQEPQAAFLVNALAVRNIAVACRQIDARLVHFS
                     TDYVFDGTARQPYSEEDRPHPLGAYAVSKLAGELYAQAYLDNALIIRTSGVFGPGGLH
                     TARSNFVELMLRLYSAGGPIRVVADHIASPTYAPQLARCTVELTEGGHCGVFHVGGGT
                     AISWFDFASMIFRAAGISDAPLRPTSEREYRTAARRPKYSALKNARLEQLGMDSMPPI
                     AAALDMYFAARQSAASS"
     gene            6065..6727
                     /locus_tag="VFQ79_06885"
     CDS             6065..6727
                     /locus_tag="VFQ79_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0139415.1"
                     /translation="MPGTRSARVPRPELLDNAEASAASRSLADLVRINRYFGGHRTVR
                     NTLRKLFAPVDSFSVLDVGAGSGDSAEAIRSAFPNAQVASLDYRLTHVRLASGPRVVA
                     SAFLLPFRPKAFDVVFCSLFLHHFDEAQIAELLKYFGSLARKAVVAVDLERHWFARRF
                     LPATNLFFSWHEITLHDGPASVESGFLPAELADLARTAGLREVQVRRHLPWFRLSMSA
                     RP"
     gene            6745..7656
                     /locus_tag="VFQ79_06890"
     CDS             6745..7656
                     /locus_tag="VFQ79_06890"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU0139416.1"
                     /translation="MKPLRLAILGILLAVIALGLYLYPPTRLAALVIAGRSPVCPLGQ
                     AVRSADNLKQQIAIKDRILEASKLLETDPEGYRLWETPKGRYWIPEGSDYVLPFNLAE
                     QERRIYGTGERGIQKGDIVLDCGANIGVYTREALDAGASKVIAIEPAPENIECLNRNF
                     RAEITEGRVIVYPKGVWDKDDFLTMHVDPHNSAADSFLIQREGSVEVARLPLTTIDKL
                     VEELKLSRVDFIKMDIEGAEPNALIGGRQTLAKFHPRLALSAYHAPDHPEKIPALVTA
                     AWPGYRMECGPCAEANGRIRPDILYFY"
     gene            7679..8653
                     /locus_tag="VFQ79_06895"
     CDS             7679..8653
                     /locus_tag="VFQ79_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0139417.1"
                     /translation="MVHVVGGGLAGCAAALTACRAGAKVRLYERSALPRHKVCGEFLS
                     PGILPQLEQLGLLESFLAAEPARIHRMVLNYGRVEKAAKLPDTAYGLSRFVLDKLLYD
                     AAVSAGVQAAGASAPMGATPAVLATGRRAVAPRGRRLFGFKSHYRGPAGDAVELYFFR
                     GGYVGVSPVESGLTNVCGLASEDILRSWNFRFDDLVASVPALNRRVAPLTRAMDWLST
                     GPLVFRTESRKNSVEGLYPAGDALAFIDPFTGSGMLIAVITGSMAGSAAAQNLPVRDY
                     LQKCARLLHRPFEFSSIFRRVLTAGVPPVLARLAPGALLYRLTRPRLS"
     gene            complement(8718..8915)
                     /gene="rpsU"
                     /locus_tag="VFQ79_06900"
     CDS             complement(8718..8915)
                     /gene="rpsU"
                     /locus_tag="VFQ79_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU0139418.1"
                     /translation="MAEVHVQDGETLESALRRFKRKVQQEDIIKEIKRHSFYLKPGEK
                     RRVKEALARKRNRKKRSRDIE"
     gene            complement(9000..10316)
                     /locus_tag="VFQ79_06905"
     CDS             complement(9000..10316)
                     /locus_tag="VFQ79_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0139419.1"
                     /translation="MERRDFWKGILGAPAISAAAAQQYEKNTRGLPALTIKDVKVIPT
                     SGGGRYRWVFLKIITGEPGLYGIGSAGNHYQTHAVIAALEKHLKPWLIGKDPNRIEDL
                     WQSAHVRTYWRNGPVNNNVLSAMDMALWDIKGKRAGMPVYELLGGKARDAVPCYDHAG
                     GSDRESAVESVRKSMENGYRFIRVQYGGNYGGGGFIPPGEGSRPEGGYNGPAFDEELY
                     VETIPPLFEYVRSKVGFGPKLCHDVHSHLTGINAVEFSRRMQLFQMLFIEDVLPPEQI
                     AWYKNIRNVCSTPQAVGEVFSHPYEYLPLVFERLIDFVRCRVSAIGGITPAKKLATLC
                     EICGVKTAFQEGGENDPVNQMASYHVDISSTGFGIQEENGFPPVVHEMMPGTAQIRRG
                     YLYGSEKPGIGVDIQEQLAAKYPLGEMRNGAAYGTDRAMDGSVVKP"
     gene            10414..10944
                     /locus_tag="VFQ79_06910"
     CDS             10414..10944
                     /locus_tag="VFQ79_06910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0139420.1"
                     /translation="MKRSQTIAIGVAVACGLFAQTPPKTHLKVGDMAPDFTLPSTAGK
                     PVTLSDFRGQKNVVLAFYPAAFTGGCTKEMQAYQLGLDKFTGAGTEVFGVSTDNTPSQ
                     RRFAEDLKVTFPMLSDFATREVSRKFGVLMPERGIANRATFVIDKEGRIQHIEEGSAA
                     IDITGAANACSRLSRR"
     gene            11053..11352
                     /gene="hfq"
                     /locus_tag="VFQ79_06915"
     CDS             11053..11352
                     /gene="hfq"
                     /locus_tag="VFQ79_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEU0139421.1"
                     /translation="MEKLAQNIQEAFLNNARKEKIFLTIYLMSGVKLSGRIKSFDKYS
                     LILESNSQEQLIFKHAISTVVISKPAHAGPHTTVGAGTGVPHAPSPGAPADSVEG"
     gene            11422..12681
                     /gene="hflX"
                     /locus_tag="VFQ79_06920"
     CDS             11422..12681
                     /gene="hflX"
                     /locus_tag="VFQ79_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU0139422.1"
                     /translation="MRRAEAVASVLDYTPGESLDELTSLAISAGAEVVERLVQSRPRM
                     DAATLVGSGKVDELAGLVQAHSAGLVLFDQELTPTQQRNLERALQAKVVDRTQLILDI
                     FARRARTREGQLQVELAQLNYLLPRLAGRGIDMSRLGAGIGTRGPGETQLETDRRRIF
                     RRIRKIEGDLNSVRAGRSTQRQKRRSVPLATIALVGYTNAGKSTLFNRLTGAGVLADA
                     RMFATLDPTVRAIALPSNRRALLSDTVGFIRSLPTTLVRAFRATLEEVTEAALLLHVI
                     DASSAHAAQQTQHVFEVLSDIGAGATAQILVLNKADRLGEAVSNAAAAARRITGSDVP
                     APAVAVSAASGLGIPDLIAAIDEALPTEPFSRALFRFPVSEGAGINYLYEHGRVTDRQ
                     DTDDYCEITADVTAAVRQRLQRYLVPE"
     gene            12762..13427
                     /gene="pgsA"
                     /locus_tag="VFQ79_06925"
     CDS             12762..13427
                     /gene="pgsA"
                     /locus_tag="VFQ79_06925"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU0139423.1"
                     /translation="MNLPNLLTLLRIFFVPLLVAALVQENRTHEILGVPVTNAFLALG
                     IFLAAAATDMLDGYLARRWKQITTVGTLLDPVADKLLVSSALISLVEVQVVSSWMVIL
                     IIGREFAVSGLRSIAAAEGYTIRASDLGKTKMATQVVAISLLMLSVQWPVLSGAADFS
                     MWVVVIFAVISAADYFRKFWRKVDVQVKERRRRELLELARNRRKARHATAAESRAGVK
                     PLG"
     gene            13459..13555
                     /locus_tag="VFQ79_06930"
     tRNA            13459..13555
                     /locus_tag="VFQ79_06930"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13493..13495,aa:Sec,seq:tca)
     gene            13672..14328
                     /locus_tag="VFQ79_06935"
     CDS             13672..14328
                     /locus_tag="VFQ79_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139424.1"
                     /translation="MKLQQADGFPLTFNLPDAAAMTGARVPALRTYIARNILNHVGQF
                     PLAGQERRFTVTGLVEVGIVDALAESLSLAAASKVADYGLEAAFSVARLCGADSHENF
                     DQKPELWMNGPQWDHRDLANPVFWAFVLNENNNVAGFDVAFGWSDIGRACKDVAKQTF
                     RVTLPSVPWGGPATQVSSLPQPSEEFIGTATPHVHVLNLTAILTKIDRAISEKQGTAS
                     "
     gene            14529..>14708
                     /locus_tag="VFQ79_06940"
     CDS             14529..>14708
                     /locus_tag="VFQ79_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0139425.1"
                     /translation="MEITEEQFAHIKDSLPVQRGNVRLTNLQVLNAILYVAEQGCKWR
                     GLPKRFGRWHTIYMRM"
BASE COUNT         2843 a         4397 c         4308 g         3160 t
ORIGIN      
        1 tgaattctct taatacgctc ccgcgccttg aattcttcat ccgggtgctg gttgcggctc
       61 atcgcgagaa acgcctgata gttctccaga gcgggcttgt agaccttcat atgatccaga
      121 gccagcgccc gcaaataata atgtgacgcc gtttcggcgc ccagtgattt cagtttatcg
      181 agagcttcga gcgcccgatc gtatttttcc agcaggatac gcgcggccgc gacctcattc
      241 catacctcga cggaccgagg ctcggccctg gccaccgctt cgaactgctc cgccgctgcc
      301 gcgtagttct tctggtcgcg caaaacccgg ccgtagtgaa gacggagccc ggtattcgcc
      361 ggctccatac gcaacgcttc tttgagcata ccaagcgcct tatccgtctc attacttcta
      421 aggtacgccg tagccagagc cagccggtta gcggctgttg gcgactgctt caccgcaaac
      481 tcgagaggcg aaatcgcttc gccgggcttg ccggtttcga tcagcaattc tcccagacgc
      541 tcttgcgctg ccgcatggtc cggaaactgg cgatagagcg cgatggcttc ctcccgctgt
      601 tgggccgact ccaaaacggt tgcgagttcc aataaagtgt cccggtagga aggatcgagc
      661 tccgctgctt tgcggtaatg cggaattgcc tccgccgggc ggccgagttt tacgaacgaa
      721 cgggccattc cgagtgcggc cggcgcggat ttggcgtcaa gctccaacgc tttaccataa
      781 ctttccgcgg ccttcgcaaa atcggccgat tgaaattgtg cctccgccaa ataaaacacc
      841 gggcgaaatt cgcctggttt ctgcgctccg gcagcctcta aaacaggcaa cgcttctttc
      901 ggccttttat tccgaagcag caaaattccc aggtttaatt gtgcctgata gaggcccggt
      961 ttcagctcca gaactttccg atattcgctg atcgcctccg aatcccgctg cagcatgctg
     1021 taggcgaaac caagatggaa gtgcgctgcg taatcgctcg aatcggcttc ggcggccttc
     1081 gaaaataact caacggcgcg ctcgtacttc tcgctttcaa gggccttcat gccctccgcg
     1141 gcgaaatcaa ttgtggtgtc cggctgggat tcggacgcgg cagcgggctt tgactgctcc
     1201 gcttccggcg ccggaggggc ctgaaatgcg gcggcatacg gagtgtgcaa gctgcacgcg
     1261 aaaaatacaa tcagccaaaa caaccacact ccttaatttt agcggttaaa gagatgcaga
     1321 tcgttaccgg tgccccgcgg cccattctgt tccacgagta aacgtcgcga cgaatccttt
     1381 ctcgcgcatg gcatcgaggt cgtgacccag agccgtgtgg aacactcttc ccgagccgta
     1441 gtccaccgtc cagaggatgg gctcgtcctt tcccgtgccg ccgatatcgg ggctgtcgta
     1501 tgcagtggcg agcaccttta cacccgggcg catccgcagc gagtgataca gttcgtcgtt
     1561 cgctaaaaaa tccgggccca agcccttcgc gataggatga tccgggtcga caaacttcac
     1621 ccggaacgaa tgccttttac cgtggcccgt tttcggagct tgttcggacc atgtagcacc
     1681 tgtcatttcg gcgtactcgg gccagggagg ttcgatgatg ccggttcgga cgtgacggtc
     1741 gccgagcgta tccagtcctc cgaacgcgta gctggcggcg tgcaccgcaa ccagaccctt
     1801 gccggagcgt acgaattcca ccagggcctt ctcggctgtt tcaccccatc gcggcccgtt
     1861 gtagtccagc accaccacat cgtagggcgc gagcgtgcgg gcggtaattc ccgcgggctc
     1921 ttcggtcaca cgcacatcga agcgcctggt agccagcagc aattcacgga gaaacggcgt
     1981 ggtggtgcgc cagtcgtggt tgttgcgtcc ggagaagatc agcgcccgga tggcgccctt
     2041 cttgaaaaac ggcgccatgt ccgtttgcgc cggagccagg caggtaagca acaggatcag
     2101 cgcgggcagg cgccgcgcca tcctgctcat acgttccact ccggccgcca ttcgatccgc
     2161 gacttcttct gataagcgat gtttccgata tggcatgcca tcgatccata atggccgtcg
     2221 accacgttcg cgttcggctc ttttcgcgac cggacgcaat cgagccagtt cgccatgtgc
     2281 gcctcttcgg ggctgcccgg cgcagtgatt tcaggagcat tccgttccct gggtatgaaa
     2341 cggtagccgc cgcgaaagat cgacaggcga ccgccggtcc ccatgaagac gatggaggcg
     2401 gcctccttcg ctaccatgtc ggaaatagag gcctcgaagg tcacgtttaa ccgctcggga
     2461 taattgagaa tcaggttgat gttgtccggc gtgtcgcggc cgtcgtcgtg ttggtagatg
     2521 ccgccgagcg caacggcgga atcgggtcgc gtcaggccca gataccagtg cacaacgtcc
     2581 accatgtgaa cgaacagccc gccggtctgc ccgccggttg aaaaatccca ataggcgaag
     2641 cggttgaagt aacgcttcgg gtcccaggga cgctttggca gccagccgag acaggcatcc
     2701 cagttgagcc ccgccggttt ccggtccatg tccggcggaa cgggagtaag atatccccca
     2761 ttggcgttcc agatggtgcg gaccatgtgg acgcgtccga tgaggccggt atcgaagaac
     2821 ctctgtttcg cctcctggaa aacggccata ctccgctgct gcacacctac ctggagcacg
     2881 cgctttgcgc tgcgcacggc cttgacaagt tcgcggccct gttcggggat ggatgtcatc
     2941 ggcttctcga catagacgtc cttacccgcc tggcaggcgg cgacggaaat cggcgtgtgc
     3001 agatggtcga aggtggcaac gatcaccgca tccacatccg tccggtccag aagctggcga
     3061 tagtcggcat ctttcttcac gggcacgccg gctgttttct ccgcttcgtc cctgcgccgg
     3121 tcccacgcat cgctcaccgc cacccactca atgccgccca ctttattcgc gacgcgcatg
     3181 aggtagctcc cacgatttcc ggttccgatg atgcctagcc ggacgcgatc gttcgcgccc
     3241 agcacgcgcg aataggacgc agccgggacg gcggacccga caaatgctct gcgggatatg
     3301 ctggatacga tcatgaaaac ctccaccttg acctcttcag ggccgcggcg aggttatttt
     3361 atcaattttt ccaggtattc gtaccttttt gacgtaaagt gatctttagg tgaaggccgg
     3421 caacccggaa ggaacaaggc acgtcttcaa ctcaggatga aaccatgcca gtcgcttccc
     3481 aggcagaatt caaacacgcg aatcgcgccc aactgagctt gttcgctcct cttgaaaaga
     3541 gggtcctggt gtggatggcg caacgcatgc cggcgtgggt aagttcggat catctgacgt
     3601 ttctcgggtt cgcagcgatg ctgcttgcag gcctttcgta ctgggcagcg agatggaact
     3661 cctacgcgct gttgctggtg gtggtttggc tggccgtgaa ctggttcggc gacagcctgg
     3721 atggcacgct ggcgagagta cgcgaccggc agcgtccacg ctacggtttc tacgtcgacc
     3781 acatcaccga tgcgttcggc accttctttc tgctgggcgg gctggcgctc tccggctaca
     3841 tgagcccgct cattgccctg gggttgctga ttgcatactt cctgctttcc attgaggtct
     3901 atctggctac ctacaccatt ggcaccttcc acctctcctt ctggaagttc agccccacgg
     3961 aactgcgcgt cctgctggcc atcggcaata tcgctttact gttccggccg gtggtcgaac
     4021 tgttcgggca caagtatctg ctgttcgata tcggcggagt ctgcggcatc gtgggtatgg
     4081 cagtgatggt ggttacggcc gccgtgcgtc acacccggac cttgtataac gccgagaggc
     4141 tgccctgatg cttctcgtgc gctggctcaa gttcaatgcg gtcgggggac tcggcattgg
     4201 ggttcaactt ggggtgctgg cgcttctggc gggtgcgtgc agcgtcaatt atcttgccgc
     4261 cacgatcata gcggtggaag cggcagtgct gcacaacttc acttggcacg agcggtacac
     4321 ctgggctgac cgcacaagcg cccctggtgg tttttggctg cggttagtgc gatttcacac
     4381 gagtaatggg ttgatctcga tcgccggaaa tgcacttttg atgcgccttc tggtgggcgg
     4441 tttgcgctgg aactatctga tagcaaatct ggttgccatc gccgcctgcg gtttactcaa
     4501 ctttgcggcg gccgagtggt tcgttttccg gcggcctgta aagctattcg cgcatctgcc
     4561 gatagcatct atgtggaaag aagacgcgaa cccagactgg cggcgagtca agccgccctg
     4621 ctgactactc tcacggagcc gtgtctgaaa agaaacggca ccatcgtcaa cgtttcggga
     4681 cggggcatta acgtttcgct tgaacaacca ctgccggtcg atgtcccggt gcgcatcgat
     4741 ctcgacgaca ccctggcctt gggtgaagtt tgctattgcg aacccgtgga agggcgatac
     4801 tccgtgggga ttcaggtgga tcaggtcctc aacgggctgg agtcgctgct tcggctcaac
     4861 gaggtactgc ttggcgaaac acctcgaaaa tcttcaggag agaactcccc cgaggctgct
     4921 cacgccctta aagacggaca ccaccagaac cagaagtagg gtgatgaaca gcagcagtcc
     4981 gatgcggtcg gcaaatctac cccttctcat gtctttttgg atgaggcgct tcatatcccc
     5041 tcccagagtc ctggcgacat agctattgac gacgatctta acgcaacggt ttcagaatgc
     5101 aagccggaaa tcggatccgc tcgtgtaaat ggtgctcagc cgtgaaataa gtctccattc
     5161 gcgctattct cgtgcgtgaa tggcaaagcg cgtggtgatc ttcggcagtg cgggacagct
     5221 cggcgtcgaa ctggttcgcg aattttctca aagaaattat tcggtcatgg ggtacgaccg
     5281 ggctacggtc gatattgccg acagctcagc cgtcgagcgg gaaatagcag cggccgatcc
     5341 ggaactggta gtgaacgccg cggcctacaa tcaggtagat cttgccgaac aggaacctca
     5401 agcagctttt ttggtgaatg ccctggcagt ccggaatatc gctgtcgcat gccggcaaat
     5461 cgatgcccgc ctggttcatt tctccacgga ctatgtattc gatggaaccg caaggcagcc
     5521 ctacagcgag gaggaccgcc cccatccgct gggagcctat gccgtttcga agctggcggg
     5581 tgagttgtat gcccaggcct atcttgacaa tgcgctcatt atccggacct cgggagtatt
     5641 cggtccgggg ggcttgcata cggcgcggag caattttgtc gaactgatgt tgcggctgta
     5701 ttcggcgggg ggacccatcc gggtagtggc ggatcatatc gcatccccca cctatgcccc
     5761 gcagcttgcc cggtgcactg tcgagctaac ggagggcggg cactgtggtg tttttcacgt
     5821 tggcggtgga acggccattt catggtttga ttttgcaagc atgatttttc gggccgccgg
     5881 aatctccgat gcgccccttc gtcccaccag cgagcgcgaa taccggactg cggcaaggcg
     5941 tccgaagtat tccgcgctca agaacgcacg gctcgaacag ctcggcatgg attcgatgcc
     6001 gcccatcgcc gccgccctgg atatgtactt cgccgcacgc caatcggccg cctcgtcctg
     6061 atccatgccc ggaacgcgct ctgcccgggt gccgcggccg gaactcctcg acaacgccga
     6121 agcgagtgcc gcttcccgca gcctggcgga cctggttcgg atcaaccggt acttcggcgg
     6181 acaccggacc gtccggaaca cactccggaa gcttttcgcc ccggtggatt ccttttccgt
     6241 gctggatgtg ggagccggct cgggagatag cgcggaagcg atccggtccg cctttccgaa
     6301 tgctcaggta gcttcgctcg actaccgcct cacgcacgtg cgactggcat cagggcccag
     6361 ggtggttgcc agcgcgtttc tgcttccttt ccgtccgaag gcctttgacg tcgttttctg
     6421 ttccctcttc ctgcatcatt tcgatgaggc gcagattgct gagcttttga agtatttcgg
     6481 ctccctcgcg cggaaggcgg tggtcgccgt tgacctggag cgccactggt tcgcccgccg
     6541 cttcctgccc gcgacgaatc tctttttttc ctggcatgaa attacgctgc acgatggtcc
     6601 ggcgtcggtt gagtcgggtt tcctgccggc ggaacttgcg gatctggcgc gcacggcggg
     6661 gctgcgggag gtgcaggtac gccgccacct gccctggttc cggctatcga tgagcgcgcg
     6721 gccgtaatgc gctatagtcg gcctgtgaag ccactccgtc ttgccattct cggtatcctg
     6781 ctggccgtca tcgccttggg cttatacctg taccctccca cccggctcgc tgccctggtc
     6841 atagccggcc gcagccctgt ttgcccgctt ggacaagcgg tcaggagcgc ggataacctc
     6901 aagcagcaga tcgcgattaa ggaccgcatc ctggaggcaa gcaagctcct ggagaccgac
     6961 ccggagggat accgcttgtg ggagacgccc aagggccgtt actggatccc cgagggaagc
     7021 gattatgtcc tcccgttcaa cctggccgag caggagcgcc ggatctatgg caccggagaa
     7081 cggggcattc agaaaggcga cattgtcctg gattgcggcg caaacatcgg cgtctacacc
     7141 cgggaagctc tcgacgccgg ggcgtccaaa gtgatagcca tcgagccggc tcccgaaaat
     7201 atcgagtgcc tgaatcgcaa tttccgcgcg gagattacgg agggccgcgt tatcgtctac
     7261 ccaaagggcg tttgggataa ggacgacttc ctgacgatgc acgtggatcc gcacaactcg
     7321 gccgccgaca gttttctgat ccaacgcgag ggttccgtgg aagttgcccg gctacccctg
     7381 acgacgatcg acaaactggt ggaggaactg aagctcagca gggtcgattt cataaaaatg
     7441 gacatcgagg gcgcggaacc gaatgccttg atcggcgggc gccaaacgct ggcgaagttc
     7501 catcctcgcc tggcgctctc ggcctatcat gctccggacc accccgaaaa aatcccggcg
     7561 ctggttaccg cggcgtggcc gggatatcgc atggaatgcg gtccgtgcgc cgaggcgaac
     7621 ggccgcatcc ggccggacat tctgtatttc tactaggagc gggaaaatta gaggcgggat
     7681 cgtccatgtc gttgggggag ggctggcagg gtgtgctgcc gcgttgacgg catgccgggc
     7741 gggcgcaaag gtgcggctct atgagcgctc cgcgctgccg cgccataagg tgtgcggcga
     7801 gtttctttcg cccggcatcc ttccccaact ggagcaactg gggctgctcg aatcctttct
     7861 ggcggcggaa ccggctcgta tccacaggat ggtcctgaac tacggccgtg tggagaaggc
     7921 ggcaaagcta ccggacacgg catacggcct gagccggttc gtactcgaca aactgctcta
     7981 cgacgcggcc gtgtcggcgg gcgtgcaagc ggccggcgca tctgccccaa tgggagcaac
     8041 acccgcggtt ctggctaccg gccggcgggc ggtggcaccc cgcggcaggc gcctgttcgg
     8101 ctttaagtca cactatcgcg gaccggccgg cgacgcggta gaactctatt tcttccgggg
     8161 cggctacgtg ggcgtgagcc ccgttgagtc aggcctgacg aatgtttgcg ggctggcatc
     8221 ggaagacatt ctgcgaagtt ggaatttccg gtttgacgac ctcgtggcgt cggttccagc
     8281 gctgaaccgg agagtagcgc cgttaactcg cgcaatggac tggctgagca ccggacccct
     8341 ggtattccga acggagtcgc gcaaaaactc cgtcgaagga ctctaccccg cgggcgatgc
     8401 cctggcgttt atcgacccgt tcactgggtc cggtatgctg atcgccgtca taaccgggag
     8461 tatggcggga tcggcggcgg cgcagaacct gccggtgcgt gactatttgc aaaaatgcgc
     8521 caggttgttg caccggcctt tcgaattttc ttccatcttc cggcgggttc ttaccgcggg
     8581 cgttcccccg gttctggcaa gactcgcacc cggagcgctg ctttaccgcc taactcgtcc
     8641 ccggctgtcc tagcacaaaa gaaaaggcag gccgaagcct gccctctcgt ttgtaatccg
     8701 gtttggactc tctcgaacta ctcaatatcc cggctccgct tcttgcggtt ccgcttccgt
     8761 gcgagcgcct cttttacacg gcgcttctca cccggtttca agtagaacga gtgtctcttg
     8821 atttctttaa tgatgtcctc ttgctgaact ttgcgcttga atcgccgcaa ggcgctttcg
     8881 agggtttcac catcctgcac atgaacttct gccaaatgtc ttcaccccct tttcgcgctg
     8941 gcaagccata gattgaatct cttccatcat cgaaggcact atatctggaa gtcaagttgc
     9001 tacggcttaa ccaccgagcc gtccatggcc cggtccgtcc cgtaggcggc gccgttcctc
     9061 atctcgccga gtgggtattt ggccgcgagc tgctcctgaa tatcgacccc tatacccggc
     9121 ttctcgctgc catacaaata gccacgtctt atttgtgccg tgccgggcat catctcgtga
     9181 accaccggcg gaaacccgtt ctcttcctgg attccaaagc cggtgctgga gatgtccacg
     9241 tgatagctgg ccatctggtt caccgggtcg ttttcgccgc cctcctgaaa agccgtcttc
     9301 acgccgcaga tctcgcatag cgtagctaat tttttcgccg gtgtgatccc gccgatcgcc
     9361 gacacgcggc accggacgaa atcgatcagc cgttcaaaga ccaggggcag gtattcataa
     9421 gggtgagaga agacttcgcc cacggcctgg ggtgttgagc agacgttgcg gatgttcttg
     9481 taccaggcaa tctgttcggg aggtaacaca tcttcgatga acagcatctg gaaaagctgc
     9541 atgcgccggg aaaattcgac ggcatttatc ccggtcaaat gggaatgcac atcgtggcac
     9601 agtttgggac cgaagccaac cttcgatcga acgtattcga agaggggcgg aatcgtctcc
     9661 acgtacagct cttcgtcgaa tgcaggcccg ttgtagcctc cctcggggcg gctgccttcc
     9721 ccaggcggaa taaaacctcc gccgccgtag tttcctccgt actgtacacg gataaagcgg
     9781 tagccgtttt ccatggactt gcggacgctc tcgacggccg attccctatc tgaccctccg
     9841 gcatggtcgt aacagggaac cgcgtcgcgc gccttgccac cgagcaattc atagacgggc
     9901 attccggcgc gcttgccctt gatgtcccac agcgccatgt ccatcgcgct caatacattg
     9961 ttgtttaccg ggccgttccg ccaatacgtg cgtacatgcg cgctttgcca cagatcctcg
    10021 atccgattcg ggtccttccc gatgagccac ggcttcagat gcttctcgag cgcggcgatc
    10081 acggcatgag tctggtagtg attgcccgcc gaaccaatgc catacaggcc gggctcgccg
    10141 gtgataatct tcaggaacac ccaccggtac cttccaccgc cactcgtcgg gatcaccttc
    10201 acatctttga tcgtgagcgc gggaaggccg cgtgtattct tttcgtactg ttgggccgca
    10261 gcggcggata tcgcaggcgc gccaaggatc cctttccaga agtctctgcg ttccataatg
    10321 agttccccgg actcctcagg atcgtatcaa atgcggtgcg ggtcgcagct tccgccacag
    10381 actgctagac tattgtcaca agctggaggg acgatgaagc gctcacaaac catcgccatc
    10441 ggcgttgccg ttgcatgcgg gctgttcgcc caaacgccgc cgaagacgca tttgaaggtc
    10501 ggagatatgg ctccggattt taccctgcca tccactgcgg gtaaaccggt gacgttgtcg
    10561 gatttccgcg gacagaagaa cgtcgtcctg gcgttctacc cggcggcgtt caccggcggc
    10621 tgtacgaaag agatgcaagc ttatcagtta ggcctggaca agttcaccgg tgccggcacg
    10681 gaggtgttcg gtgtcagcac cgacaacact ccatcccaaa ggcgctttgc ggaagacctc
    10741 aaggtcacgt ttccgatgct gagcgacttt gccacgcgtg aggtttcgcg aaagttcggg
    10801 gtcctgatgc ctgagcgcgg cattgctaac cgggcaacct ttgtgattga caaagagggc
    10861 cgcatccaac acattgaaga aggctctgcc gctatcgaca tcacgggagc ggcaaatgcc
    10921 tgtagccggt tatcgcgccg gtgagttccc taccggccgc tgctcagggc cgtatcggga
    10981 atatgttgta caatctggaa cgccaaggga ttcgaggagc cttggcctat tccgccgagt
    11041 aaaggaaaac agatggaaaa gctggcgcaa aacatacaag aagcttttct aaacaatgcg
    11101 cgcaaagaga agatctttct gaccatttat ctcatgagcg gggtgaagct gtccggcaga
    11161 attaaaagtt tcgacaagta ctcgctcatt ctggagtcta atagccagga gcagttgatt
    11221 ttcaaacatg caatttcaac cgttgtaatc tccaagcccg cccatgccgg cccccataca
    11281 acggtgggcg ccggaacggg tgttccccat gctccctcac ccggcgcacc agcggattcc
    11341 gtggagggtt gaatttcagc ggcagcgaac ccggctaaag agagagcagt actggttggc
    11401 ctccatataa aagaccgcca gttgcggcgc gctgaggctg tcgccagcgt actcgattac
    11461 actcccgggg aatcgctcga cgaacttact tcgctggcta tcagtgccgg agccgaagta
    11521 gtggagcgtc tcgttcaatc gcgtccgcgc atggatgcgg ctaccctcgt cggttcagga
    11581 aaggttgacg agcttgccgg tctggtccag gcgcattcag ccggccttgt gctttttgac
    11641 caggaactga cgccaacaca acagagaaac ctggaacggg cgttgcaggc taaagttgtg
    11701 gaccgcacgc aattgattct ggacatcttt gcgcgaagag cacgcacccg tgaggggcag
    11761 ttgcaggtgg aactggccca gctcaactac ctgctccccc gcctggccgg ccgcggcatc
    11821 gatatgtctc gcctcggcgc aggcattgga accaggggtc ccggcgaaac gcaattggaa
    11881 acagaccggc ggcggatttt tcgccgcatc cggaaaatcg agggcgatct caacagtgtt
    11941 cgtgccggcc gttctaccca gcggcaaaaa aggcgatcgg tgccgctggc caccattgcc
    12001 ctggtcggat acacgaatgc cggtaagtcg actttgttca accgattaac cggcgcggga
    12061 gtgctggcag acgcgagaat gttcgccacg ctcgacccca cggtacgcgc gattgctttg
    12121 ccgtccaacc ggcgcgctct gctgagcgat accgtcggtt ttatccgcag cctgccgaca
    12181 actctggtgc gggccttccg cgccacgctc gaagaggtca ccgaagcagc gttgttgttg
    12241 cacgtgatcg acgcctcgtc ggcgcacgct gcccagcaga cgcagcatgt ttttgaggtt
    12301 ctctccgata ttggcgccgg agccaccgcg cagattctgg tcctgaacaa ggcggatcgc
    12361 cttggagagg cggtttccaa tgcagccgca gccgcccggc gcattacagg cagcgatgtc
    12421 cccgcgcctg ccgttgcggt ttctgccgct tccggcctgg gcattccgga tctgatcgca
    12481 gcgatcgacg aggcgcttcc gaccgagccc ttttcgcgcg ctctgttccg gtttccggtc
    12541 agtgaaggcg ccggcatcaa ctacctgtat gagcatggcc gtgtgacaga ccggcaggac
    12601 acagacgatt actgcgagat tacagcggac gtcacggcag cggtgcgcca gcggctgcag
    12661 cgttatctgg tcccggaata aaagcgatgc cttttcaacg taaaaccggt acgcgccggt
    12721 tggcaaacca gttgaatcgg ggtttatagt tgagttgacg gatgaatctg ccgaaccttc
    12781 tgacgctgct ccgtattttc tttgtcccgc tgttggtagc ggcgctggtt caggagaacc
    12841 gaacacacga aatcctcgga gtaccggtaa cgaatgcttt cctggcgctg gggatcttcc
    12901 tggccgcggc cgctacggat atgctggacg gctatctcgc gcgccgctgg aagcagatca
    12961 ccacggttgg cactctgctg gaccctgtcg ccgataagct tctggtgtct tcagccttga
    13021 tctccctggt ggaagtgcag gttgtctcaa gctggatggt catcctcatc atcggccgcg
    13081 aattcgccgt ctcgggctta cgaagcatcg ccgcggcaga aggctacacc atccgggcaa
    13141 gcgatctcgg taaaaccaag atggccaccc aggtagttgc tatttctctt ttgatgttga
    13201 gcgtacagtg gcctgtcctc agcggagccg ctgatttctc catgtgggtg gtggtgatct
    13261 ttgccgtgat atcggcggcc gattacttcc gcaagttctg gcgaaaagtg gatgttcagg
    13321 tgaaagagcg ccggcggcgg gaactgctgg aactcgcccg gaatcgccgg aaggcccggc
    13381 acgccaccgc agcggaatcc cgggccggcg taaagccgtt aggctagcag cgcctttcgc
    13441 aatggtagaa tctcccctgg aagctagcct tggccggtgc cgggcctggt cttcaaaacc
    13501 agtgtgcggc gcgacgagtg tcgcgggtgg gttcgactcc cactagcttc cgccaaacaa
    13561 accattgaaa acaagtcatt tgtaagcaga aatcgaaaac gtaccaaacc attgccttct
    13621 gttgtgacaa aacaatttga tatgacatgt cagatcgttt acagtggggc tatgaaactt
    13681 caacaggcgg atgggttccc actgactttc aatcttcccg atgctgcggc tatgaccggc
    13741 gctcgcgttc ccgcgctcag gacctacatc gcgcgcaata tccttaacca cgttggtcag
    13801 tttcctctcg ccggccaaga gcggcggttc accgttaccg ggctggtgga ggttggcatc
    13861 gtcgatgccc tcgccgaaag cctgtcgttg gccgccgcct cgaaggtcgc ggattatgga
    13921 ctcgaggcgg cgttctcagt ggctagactg tgtggggcag actcgcacga aaacttcgat
    13981 caaaaacccg aactctggat gaacggtccc cagtgggatc acagagacct cgccaatccc
    14041 gtcttctggg cgttcgtgct caatgagaac aacaatgtcg ctggctttga cgtggccttt
    14101 ggttggtcgg acatcgggcg tgcttgcaaa gacgtggcca agcaaacatt tcgtgtcacc
    14161 ctcccttctg tgccctgggg cggaccagct acccaagtgt catcgctccc tcagccgtcc
    14221 gaagaattca ttgggacggc aaccccgcat gttcacgtcc tcaacctgac cgcaatcctc
    14281 acgaagatcg accgcgccat cagcgagaag caagggactg cgtcttgatt gccgcggcct
    14341 gccccaaacg tctctcgggc gcaaccgaga ccccaaaaga cgcgggagaa ccgtatcgtg
    14401 tccctgtaag gtttccgaat agggagttag cctaccacgc cacgcgcgat caggaggccg
    14461 agttgcgcgc ggccgaccta gggcgtgttc acactaatag aacacaatga tggtaaaatg
    14521 tttggaggat ggaaatcacg gaagagcagt ttgcgcacat taaggatagc ctgccggtgc
    14581 agcgcggcaa tgtccgtctg accaatctgc aggtgctcaa cgcgattctc tatgtggccg
    14641 agcagggttg taagtggcgc ggacttccca agcggttcgg ccgctggcac acgatctaca
    14701 tgcggatg
//
LOCUS       DASTMK010000157         9375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_8862, whole genome shotgun sequence.
ACCESSION   DASTMK010000157 DASTMK010000000
VERSION     DASTMK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8862"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1860
                     /locus_tag="VFQ79_06945"
     CDS             <1..1860
                     /locus_tag="VFQ79_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139426.1"
                     /translation="TWFGFDSQVGGSRSGNPPDAPITFPAAGVKVEGGDKGISPAIEG
                     LSVSGLFSLASGHWGDFNRGDWTLREVVTKVKGPHELIFGGEVVRLIQDISNTNTQSG
                     SFNFSSQFSGSNLADFLLGWATSWNQGAGQYQNVRGAKFSMFIQDNWRVTPSLALNMG
                     LRWDPFFPYTEIYNRVPCWSPGQQSTVYPNAPAGIIYGGDTGCPWGKGANASAGNFAP
                     RFGFAYRVHRNTVIRGGVGVYYVIPPSRIFNGPNSVAPFMPRYLLSGMLRFEDPYGSF
                     GMANPFPAEYVGAAGAQVSNDVKFAVPLQIYGSFSGNYRLTTLGVWNLRVERQFGSDW
                     MVSAAYVGNGVSHLSISGQMNPAVYIPGASTVGNTQTRRLYKDFTSVAETWTDGNAGY
                     HALQLNLEKRFSRGLSLLSNYAWSKKMDNAGTITPFRGRDFNYGRASDDIPHIFHFTA
                     LWEVPRSRFQGAMGRILNGWDLSSVTTWQSTAPFTVTSGVDNSFTAVGGDRADFIGTN
                     LNEAKLSGQSHAEMVARYFNTSLFVANKVGTYGNTGKNILRGPRFFNTDVGVIKNTNI
                     TEQVRTQFRVEFFNLFNKPNFDAPSSSLTSASFGQITSARDPRILQLTLKLMF"
     gene            1923..2810
                     /locus_tag="VFQ79_06950"
     CDS             1923..2810
                     /locus_tag="VFQ79_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0139427.1"
                     /translation="MRKMAVLLCVLSGMLCVAAEDPITKLKVAAVQFRSSSDLDDNAT
                     RMCEILGRLADEGVQVAIFPECALTRYNADSIASKSAEAIAAAEEKIRACCGQKKIAA
                     IVGSVYKVNGRTYNSAVVFSSRGELVERYAKIYLAGEKWATPGNHISFFDLEGIPSTV
                     MICHDERYPELVRLPAIQGARIVYYISHESGLRRDYKLAGYRAQLMARAVENNMFIVS
                     SNAPADPKDFTGSHGQSRIVNVDGNVLKEASFFSEEILIETLAVKPAKLERPLKELMK
                     AWWQEGVDHMMKNRTRPLD"
     gene            2821..4782
                     /locus_tag="VFQ79_06955"
     CDS             2821..4782
                     /locus_tag="VFQ79_06955"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0139428.1"
                     /translation="MQGRTVRACVLLGCAWPVLCQTDPRQLVPFLEEQIQAPEVTAYQ
                     LRRYILARIPRLPAPASSGQWTAQAGRLRQSVLNNAVLHGWPKEWVDAPPRFEDLGSV
                     PGGKGYRIRKLRYEIVPGFAGVAILYEPEEIRGKIPAILNVNGHVGAEGKAVEYKQKR
                     CINQALRGILSLNLEWFACGELLQKGNEHSYGAHLDLAGANGVGLFYLAMRRGLDYLY
                     AHPVVDRSRIGMTGLSGGGWQTIVLSALDERVRVAVPVAGHASYLSRIERMRDIGDME
                     QNATDMFASIDFAHLAAMRAPRPTLLIYNAEDSCCFRAPFVKRENFDAVAPFFRLYGR
                     EDSLGWHENTDPSTHNYQLDNRLQAYRFFEKHFALPASGGEIPVDHHIHSAAELTVGL
                     PKENLTILGLARKLAEGIQRPPLPSGEAAIRAWRQQQRDVLKETVRFRPVEVAHAWIA
                     GNSKHRGLETLSYRLEFSNQLSAAGTWLKAIASPENAAATLTIADGGRKTSQSEVSDR
                     LNRGEQVVAMDLLLMGEMSPGPKAGGSNFIGILSALGERALGIQAAQLIATGHWAQKL
                     SGRPSVRLEASGMRSQVVALVAAALEPGLFSELVVRNGVPSLRHLFDGPVEPRAAPEL
                     FCLDLYKRFDIPELTRLASDAVVRNETTR"
     gene            5031..7298
                     /locus_tag="VFQ79_06960"
     CDS             5031..7298
                     /locus_tag="VFQ79_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139429.1"
                     /translation="MSRCQGADRLEGNGFAPDAIRAELERILRSELFACSGRLSRFLR
                     FAVEQALAGRAEDLKESVLAVEVFDRQATFDSHVSSIVRVEARRLRERLQRYYDSEGR
                     EAIIRIDLPKGRYSPAFRKVERPGTQPLEVVESPAAPREDARQARRVIHVKLMIVVAC
                     AAVAAAAWLATSLEIPRRTISEPVVVRLTSDTGLTCQPTLSLASKLIAYTSDRAGDGR
                     FDIWAQPIDGGRPSRLTDDQGNNTEPAFSPDGSTLAYRSDRDGGGIYLLSSLGGKSTL
                     LARGGLRPRFSPDGRFVAYWDGERSFRSARIFVISVAGGQPAQLVPGFQYAAYPVWSP
                     DGHKVLFVGISRAGEQWDWWLTPINGGAPVRTGIREVFARQGLESPDRGWGSVPRFAP
                     GGWTRNGYVVFSARSGRYTNLWRIRLSPKDGRPAGEAERLTSGAGCEDHVSVAGEWVV
                     FSVVAKKSDIWRMTLDENTGEPTGVPVRLTNNAAANVWPQVSNDGRTLVFLSDRDGNY
                     DVFVRNMLTGEDKPLTSTRQDEFCALISMDGSQVAFQRSGQPKPSIYVVPVSGGEAKV
                     VCEDCGEPRTWSPDNRILLYQNGASSHSRIGVLERPGMGTQWLGDPEQSFYSPRFSPD
                     GRWIAFVARTPPERHRLVIVPFRKQAAGSRHEWISLEGSGYWVDKPRWSPDGNLLYFL
                     SDRDGSVCVWAQRLSATDKRPSGEPFPVWHMHATRDSLASTVGRELAVARGSVFLSLA
                     HSLGNVSMMTRSGRD"
     gene            7541..7795
                     /locus_tag="VFQ79_06965"
     CDS             7541..7795
                     /locus_tag="VFQ79_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139430.1"
                     /translation="MPAPEELAYGNVLQLAGAVVLFCWTAAFAAGQNVLHVPAGGNLQ
                     QAIDAALPGDTIAIKPGENLPVINTKSVGKLPVAILSSAT"
     gene            7808..8038
                     /locus_tag="VFQ79_06970"
     CDS             7808..8038
                     /locus_tag="VFQ79_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139431.1"
                     /translation="MVDSTSLTFGSTGRERSLAFCNPNGEDVNGDGRLDLMCHFNTAG
                     TALKSSDKQAVLKGRTVTDGLIEGSQEIRIVH"
     gene            complement(8062..>9375)
                     /gene="secF"
                     /locus_tag="VFQ79_06975"
     CDS             complement(8062..>9375)
                     /gene="secF"
                     /locus_tag="VFQ79_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU0139432.1"
                     /translation="LFLFGTGPVRGFAVTLVIGLIANLFTAVFVSRSIFDLKLWRHPR
                     LSTLSIGKELFQQTSIDFLSKRALTLGLSAAAIAISIGALAMKGGPRYGLDFRGGTEM
                     YVRFDPRPPIDDVREALSELNASLQETTAGRGEFIIGTELSDESSLEQARQAVEGALR
                     EKYANLGGKLDLNNASQSTLADRLRDTLPSAGVNLGEEELQALVSRILGYRDREKGGI
                     IRNLDELAAVPGLDQNVLNTIRQECGVGSFNIRQVGIVGPRAGEQLRRQALLATACAL
                     GGMLIYIAFRFQLISGAAAVIATIHDVIITLGLFAITNREIDLTIIAALLTLIGYSMN
                     DKIVVFDRVRENMRGKRIRGTSFLDLVNQSINQTLSRTLLTAGPTLLACIALYFLGGE
                     VLNGIAFALFVGIVVGTYSSIFVASALLVMWEQYRSAPKPARAAA"
BASE COUNT         1893 a         2749 c         2942 g         1791 t
ORIGIN      
        1 acgtggttcg ggttcgactc gcaagtgggc ggctcacgct cgggcaaccc gcccgatgct
       61 ccgatcacct tcccggcggc aggagtgaag gtcgagggag gagataaggg catttcgccg
      121 gccatcgaag ggttgagcgt atcgggatta ttcagtctcg cgagcggcca ctggggcgac
      181 ttcaaccgcg gcgactggac actccgcgag gtggtgacca aggtgaaagg accgcacgag
      241 ctgatcttcg gaggcgaagt agtgcggctg atccaggata tctccaacac caacacgcaa
      301 tcgggcagct tcaatttctc cagtcagttc tccggttcga acctggcgga tttcctgctt
      361 ggctgggcga cgagctggaa tcagggcgca ggacagtatc agaacgtgcg cggagccaag
      421 ttcagcatgt tcatccagga caactggcgg gtgacgccaa gtctggcgtt gaacatggga
      481 ttgcgctggg acccgttctt cccatatacg gagatctaca accgcgtgcc atgctggtcg
      541 ccagggcagc aatcgacggt gtatcccaac gcgccggccg ggatcatcta cggtggcgat
      601 accgggtgcc cctggggcaa gggagcaaac gcctcagcgg gcaatttcgc accgcgcttc
      661 ggcttcgcct atagagttca tcgcaacacg gtgatccgcg gcggggtcgg ggtctattat
      721 gtcattccgc ccagcaggat ctttaacggg cccaacagcg tggcgccatt catgccccga
      781 tatctgctct caggcatgct tcggtttgaa gacccttacg gcagctttgg aatggcgaat
      841 ccgttcccgg cggaatacgt gggcgccgcc ggagcgcagg tgtcgaatga cgtcaagttc
      901 gccgtccccc ttcagatcta tggctccttt tcgggaaatt atcggctgac cacgctaggt
      961 gtctggaacc tgagagtgga aaggcagttt ggctccgact ggatggtcag cgccgcttac
     1021 gtcggcaatg gcgtctccca tctctcaatt agtgggcaga tgaacccggc tgtttacatt
     1081 ccgggcgcct cgaccgtggg caacacgcag acgcgccggc tgtacaagga tttcacctcc
     1141 gtggccgaga cttggacgga cggcaacgct ggatatcatg cgctccagtt gaacctggag
     1201 aagcgcttca gcagaggatt gtctctgctg agcaactacg cgtggtccaa gaagatggac
     1261 aacgccggga ccatcacgcc gttccgcggc cgcgacttca attacggccg ggcgagcgat
     1321 gacatcccgc acatcttcca cttcacggcg ctttgggagg tccccagatc caggttccag
     1381 ggcgccatgg gccgcatact caacgggtgg gatctaagtt cagtgaccac gtggcaatcc
     1441 acagctccgt tcacggtaac gagcggagtg gataattctt ttaccgctgt tggcggcgac
     1501 cgggccgatt tcattggcac gaacctgaac gaggcgaagc tgagcgggca gtcgcacgcc
     1561 gagatggtcg cgcggtactt caacacgtcc cttttcgtgg ccaacaaggt cggcacttac
     1621 ggcaataccg gaaagaacat cctgcggggg ccgcggttct tcaacaccga cgtgggcgtg
     1681 atcaagaaca ccaacatcac ggagcaggtg aggacgcaat tccgggtgga gttcttcaac
     1741 ctcttcaaca agccgaactt cgacgctccg agcagctcat tgacttccgc aagtttcgga
     1801 cagatcacct cggctaggga tccccggatc ctgcagctta ccttaaagtt gatgttttga
     1861 ttccaggacg gggcgtcgaa aacgtcccgt cgcatttgaa tttggcgacg gaaaggcaag
     1921 cgatgagaaa aatggctgtg ctgttgtgtg ttctgagcgg catgctctgc gtggccgccg
     1981 aggatccgat caccaaactg aaggtggcgg ccgtgcaatt ccggtcgtcg tccgatctcg
     2041 acgataacgc cactcggatg tgcgaaattc tggggcggct ggccgatgaa ggggtgcaag
     2101 tggcgatctt tcccgagtgc gccctcaccc gctacaacgc ggactcgatc gcaagcaaga
     2161 gcgccgaggc gatcgcggca gcggaggaga agatccgcgc ctgctgcggc cagaagaaga
     2221 tcgccgcgat tgtggggagc gtatataaag tcaacggccg cacatacaac agcgccgttg
     2281 tgttcagctc ccggggcgag ttggtcgaac gctatgccaa gatctacctg gcgggcgaga
     2341 aatgggccac tccgggcaac cacataagct tcttcgatct ggagggaatc ccctccacgg
     2401 ttatgatctg tcatgacgag cgctaccctg aactggtccg gcttcccgct atccagggcg
     2461 cgcggatcgt gtattacatc agccacgagt ccggtctgcg gcgggactac aaactcgcgg
     2521 ggtatcgcgc gcaacttatg gccagggccg tggagaacaa catgttcatc gtgtcgtcca
     2581 acgcccccgc cgatcccaag gacttcaccg gatcgcacgg ccagagccgc atcgtgaatg
     2641 tggacggcaa cgtactcaag gaagcttcct tcttcagcga ggaaatcctc atcgagacgt
     2701 tggccgtgaa gcctgccaag ctggagcgcc cgctgaaaga gctcatgaaa gcatggtggc
     2761 aggagggagt tgaccacatg atgaagaacc gcacgaggcc tctggattag gacagctccg
     2821 atgcagggga gaactgtccg cgcgtgcgtg ctactgggct gtgcgtggcc ggtgctgtgc
     2881 cagaccgatc cgagacagct cgtcccgttc ctggaggagc agatacaggc tccggaagtc
     2941 accgcgtacc aattacgccg ctacatcctc gcccggattc cacggcttcc agcgccggct
     3001 tcgtccgggc agtggaccgc gcaagccggg cggttgcgtc agagcgtcct taacaacgcc
     3061 gtgctgcacg gttggcccaa ggagtgggtc gatgcgccgc cgcgattcga ggacctggga
     3121 tcggttccgg gcggcaaggg ctaccgcatc cgtaaattgc ggtacgagat cgtgccgggg
     3181 ttcgcgggcg tcgccatcct ttacgaaccc gaggagatca gaggcaagat ccccgccatc
     3241 ctgaatgtga acggtcacgt cggcgcggag ggcaaggcgg tggagtacaa gcagaagagg
     3301 tgcatcaatc aagcattgcg cggaattctc tccttgaatc tggaatggtt tgcgtgcggc
     3361 gagttgctcc agaaggggaa cgaacactcc tacggcgccc atctcgacct ggcgggtgcc
     3421 aatggagtgg gcctgttcta tctggcgatg cgccggggcc tggactatct gtatgcgcac
     3481 cccgtggtag accggagccg catcgggatg accggtctgt ccgggggcgg atggcagacc
     3541 atcgtgctca gcgcgctgga tgaacgggtc cgtgtcgctg tgccggtggc cggacacgcg
     3601 tcgtacctct cccgaatcga acgcatgagg gatatcggcg atatggagca gaatgccacc
     3661 gacatgttcg cctccatcga ttttgctcac ctcgccgcca tgcgcgcccc gcgtccgacc
     3721 ttgctcattt acaacgccga ggatagttgc tgtttccgcg ccccctttgt gaagcgcgaa
     3781 aacttcgatg cggtggcgcc gtttttccgc ttgtatggcc gggaggatag cttgggatgg
     3841 cacgaaaata ccgacccgtc cacgcacaat tatcagctcg acaaccggct gcaggcctat
     3901 cggttctttg agaaacactt cgccctgcct gctagtggtg gagagatccc ggtcgaccac
     3961 cacatccaca gcgccgcgga gctgacggtg ggacttccca aggagaacct gacgattctc
     4021 ggccttgcca gaaagctggc ggaaggcatt cagaggccgc ccttgccctc cggagaggct
     4081 gcgattcgcg cctggcggca gcagcagcgc gatgttctga aagaaacggt ccgtttccgg
     4141 cctgtcgagg tggcgcacgc atggatcgcc ggcaacagca agcacagggg cttggagaca
     4201 ttgtcgtacc gcttggagtt cagcaaccag ctcagcgccg cgggaacctg gttgaaagcg
     4261 attgcgtctc cggaaaacgc cgccgccacg ctgaccatcg cggatggcgg caggaagacc
     4321 tcccagtcgg aggtttcgga tcgcctcaac cgcggcgaac aggttgtggc tatggatctg
     4381 ttattgatgg gagagatgtc gcccgggcca aaagccggcg gatccaactt catcgggatt
     4441 ttatccgcac tgggtgaacg cgcgttgggc attcaggcgg cacagctcat cgcgaccggt
     4501 cattgggcac agaagctatc gggtcgccca agcgtcaggc tggaggcgtc cgggatgaga
     4561 agccaggtag tggcgctggt ggcggcggcg ctagagcccg gactattctc cgaattggta
     4621 gtcagaaacg gcgtgccctc gctgcggcat ctgttcgacg ggccggttga gccgcgcgcc
     4681 gcgccggaac tgttttgcct ggatctctac aagcgatttg acattccgga gctcacaagg
     4741 ctggcgtccg acgccgtcgt gcgcaacgag accaccagat gagccgcccg attcgagtct
     4801 gggcagagtg agcaaatttc cggccgtgcc ggttcagcat cctgaacttt gccgtctggt
     4861 ctcccacacc cttcgacaag gcctctggcg gttcgcagtt gtcaacgcaa agcgacgatg
     4921 tgatagaatc actctgcctt agggagtacc ccacacactc agcacttggg cacactcagc
     4981 acttggggat agatatggca ccaggtggaa agagggcaga accgcccgac atgtcacggt
     5041 gccagggcgc ggacagactg gaaggcaatg gcttcgcacc tgatgcgatt cgggcagaac
     5101 tcgagcgtat tctgcgaagc gagctgtttg cctgctccgg gcgtctcagc cggtttcttc
     5161 ggttcgcagt tgagcaggcg ctggccgggc gagcggagga tttaaaagag tccgttctgg
     5221 cggtggaggt gtttgaccgg caggcgacct ttgattcgca tgtcagctca atcgtccgcg
     5281 tggaagcacg cagactgcga gaacggctgc aaaggtacta cgatagcgaa ggacgcgagg
     5341 cgatcattcg catcgatctg cccaagggga ggtatagccc ggcattccgg aaggtcgagc
     5401 gtcctgggac acagccgttg gaagttgtgg agtctcccgc tgcgcctcgg gaggacgcgc
     5461 gacaggcgcg tcgcgtcatc catgtcaagc tgatgattgt ggtcgcatgc gcggcggtgg
     5521 ccgctgcggc gtggttggca acgtcactcg agattccgcg ccgcaccatc tcggaaccgg
     5581 tggtggtcag acttaccagc gatacaggac tgacctgcca gccgaccctt tccctggcat
     5641 cgaaactgat tgcgtacacc tccgaccgcg ccggagacgg ccgatttgat atctgggcac
     5701 agccgattga cggcggccgg ccgtcgcggc tgacagatga tcagggtaac aatactgaac
     5761 ccgcgttctc gcccgacggc agcacacttg cttaccgctc ggaccgtgac ggcggcggca
     5821 tctatctcct ttcctccctc gggggcaaga gcacgctgct agcgagaggc gggttgagac
     5881 cacgcttctc tccggacgga cgattcgtcg cttactggga tggagaacga tcctttcgca
     5941 gcgcgcgaat tttcgtcatc agcgtggcgg gtggccagcc ggcacaactt gttcccggct
     6001 tccaatatgc ggcctacccc gtatggtcgc ccgatggcca caaggtactg ttcgtcggca
     6061 tcagccgtgc cggcgagcag tgggactggt ggctcactcc cataaacggt ggagcgccgg
     6121 tcagaaccgg gattcgcgag gttttcgcca ggcaggggct ggaatcgccg gaccgggggt
     6181 ggggatcggt cccacgtttc gctcccggag gctggacgcg gaacggctac gtcgtcttct
     6241 cagcacgctc gggccgctac acgaatctct ggcgcatccg cctttccccc aaagacggga
     6301 ggccagccgg tgaggccgag cggttaacat ccggcgccgg gtgcgaagac catgtttccg
     6361 tcgccggcga atgggtggtg ttctcagttg tcgcgaagaa atcggacatc tggcgcatga
     6421 ctctagacga gaacacaggc gaaccaaccg gcgtcccggt ccgcctgaca aacaatgctg
     6481 cggctaacgt ctggccccag gtgtcgaacg atggcaggac gcttgtattt ctctccgatc
     6541 gcgacggcaa ctacgatgtc ttcgtgcgga acatgctgac cggagaggac aagcccctca
     6601 cgtccacacg gcaggacgag ttttgcgcgc ttatctcgat ggacggatcg caagtagcgt
     6661 tccagcgcag cgggcagccg aaaccatcga tttacgtcgt gccggtcagc ggcggcgaag
     6721 cgaaagtagt ctgcgaggat tgtggagagc caaggacatg gtcacccgac aatcgcatct
     6781 tgttgtatca aaacggtgct tcgagccatt cgagaattgg ggtgctcgag agacccggca
     6841 tgggcacgca atggttgggc gatccggagc agagcttcta ttccccgcgt ttttcacctg
     6901 acggccgctg gattgcgttc gtcgccagaa cgccgccgga gaggcatcgg ttggtgatcg
     6961 tcccgttcag aaaacaggcg gccggtagtc ggcacgagtg gatttcactc gaaggatccg
     7021 gatactgggt ggacaaaccg cgctggtctc cggacggcaa tctgttgtac tttctttcgg
     7081 atcgggatgg ttccgtttgt gtttgggccc agcggttgag tgccacggac aagcgcccgt
     7141 caggggagcc gttcccggtg tggcacatgc atgccaccag ggactctctg gcgtctaccg
     7201 tcggccggga acttgcagtg gcgcgaggga gcgtcttttt gagccttgcc cattctcttg
     7261 gcaatgtctc catgatgacg cggagcggcc gggattgacg cccgccgccc gtcgatcctg
     7321 gcagctccgc gtggctgcgg gcccacccgc gtcaggcgaa gggggccaga tgcctcagcg
     7381 atttagcgac ccagtgcgca gagcatcccg gccgcgtaac gccatgtaac gggttatggc
     7441 atgacgatgg cgattcgcca acgatcagtg tgcccaggtg aagttcatcg ggagaaaatg
     7501 aacgatgcct acacatggaa atagaacatc cacttctcga atgcctgcgc cggaagagct
     7561 ggcttacgga aacgtgcttc agcttgctgg cgctgtagtg ctgttttgct ggaccgcggc
     7621 gttcgctgcc ggccagaatg tcctgcatgt ccctgcgggc ggaaacctcc aacaggccat
     7681 tgatgccgcc ctgccgggtg acacgatcgc gatcaagccg ggtgagaacc tgccggtgat
     7741 caacacaaaa agcgtaggaa aactgccagt ggcgattctt tcgagcgcca cctgaccggc
     7801 tccagccatg gtcgatagca ccagccttac ctttggctcc acgggccggg agcgcagcct
     7861 ggctttctgc aaccccaacg gggaggatgt gaacggagac ggacgcctcg acctgatgtg
     7921 ccatttcaat accgccggta cagcgttgaa gagcagcgac aaacaggcag tcctaaaagg
     7981 aaggaccgtc acggacggcc ttatcgaagg aagccaggag atccgcatcg ttcactagaa
     8041 gcaagtgagc cttgccgatc cttaggcggc ggcgcgggcc ggtttgggtg cgctgcgata
     8101 ctgctcccac atcacgagta gggcgcttgc gacgaaaatc gacgagtacg tgcctacaac
     8161 aatgcccacg aatagcgcga aggcgatccc gttaagaacc tcaccgccaa gaaaatatag
     8221 agcgatgcaa gccagaagag ttggccctgc cgtaagcagg gtccggctga gcgtctgatt
     8281 gatcgactgg ttaaccagat ccaggaacga cgtacctcga atcctctttc ctcgcatgtt
     8341 ctcccgcaca cggtcgaaca cgacgatctt atcgttcatg gagtagccga tgagagtcag
     8401 cagcgcggcg atgatcgtca agtcgatctc gcggttggtt atagcgaaca ggccaagcgt
     8461 gatgatcacg tcgtgtatcg tggcaatgac ggctgccgct ccggaaatca actggaagcg
     8521 gaatgcgata tagatcaaca tgcccccaag ggcgcaggcc gtcgcgagca gggcctgccg
     8581 ccggagctgc tccccggcgc gcggtccgac aatccccacc tggcggatat tgaagctgcc
     8641 aactccgcac tcctggcgaa tcgtattcaa cacgttctga tccaggcccg gcacggccgc
     8701 aagttcatcc agattgcgga taatgccgcc tttctcccga tcgcgatagc ccaggatccg
     8761 cgataccaaa gcttgaagct cctcctcgcc aagattcaca ccggccgagg gaagagtgtc
     8821 acgcagacga tctgccagtg tcgactggct ggcgttattg agatcgagct tgccacccag
     8881 attcgcgtat ttctcccgaa gcgcgccttc tacggcttgc cgggcttgct ccaggctgct
     8941 ttcatccgac aactctgtgc cgatgatgaa ttcgccccgg cccgcggttg tctcctgcag
     9001 gctggcattc agttccgaaa gagcctcccg gacatcgtcg atggggggcc tggggtcgaa
     9061 ccggacgtac atctcggtgc cgccgcggaa gtccaggcca tacctcgggc cgcctttcat
     9121 cgcaagagct ccgatactga tcgcgatagc cgcggcggag agccccagtg tcaaggcgcg
     9181 tttcgaaagg aagtcgatgc tggtctgctg gaacaattct tttccgatgc tgagcgtgga
     9241 caggcgcgga tgccgccaca gcttcagatc gaagatggat cgcgagacga ataccgcggt
     9301 aaacaggttc gcgatcagtc cgatgacgag ggtcacggca aagccccgca ccggaccggt
     9361 gccgaacagg aatag
//
LOCUS       DASTMK010000158         4853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_231951, whole genome shotgun sequence.
ACCESSION   DASTMK010000158 DASTMK010000000
VERSION     DASTMK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231951"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..1197)
                     /locus_tag="VFQ79_06980"
     CDS             complement(454..1197)
                     /locus_tag="VFQ79_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139433.1"
                     /translation="MPFDHSESGATAPQGKVPKKPAGKIRTEHCYCSHPAADHDSESG
                     CSQCGCQQHQARPCVCGHGAAQHNPACESCDCRQFREDGSPEFLLRRGIRAGRWQFIT
                     SEQLRFLMRPDQDFQVRVWACGMLHSVGQQRRLAVKWVNGKPATVPLSPGDIVAELNA
                     LDMIGCATKQNVRRAIADLEAQGAARRVGRHRNNVRPELLHERNRREWESWLADPDTS
                     EDDKAFARRILQENAPDWRQTRRRGWERG"
     gene            1446..2270
                     /gene="uppP"
                     /locus_tag="VFQ79_06985"
     CDS             1446..2270
                     /gene="uppP"
                     /locus_tag="VFQ79_06985"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEU0139434.1"
                     /translation="MPLYQVITLAIVQGFTEFLPISSSAHLALAPWLAGWKDQGLTFD
                     IALHFGTLAAVAIYFFRDWLQITASGFGIDYGNDPSLKQNPRLFWLLVAATIPLGVFG
                     LLAKEYAETVWRTPYVIGTMLILVGILMLVAEHTARLKKDIDRISAADAMAIGVSQAL
                     AVVPGTSRSGITITAGLFRGITRPAAARFSFLLSTPAIAAAALSAFKDLLESGQPIDL
                     MPFIVGMSVSALTGILVIAFFLRFLRNHSLKFFIYYRIVFGIIVIALAAFFRHPAG"
     gene            2267..4660
                     /locus_tag="VFQ79_06990"
     CDS             2267..4660
                     /locus_tag="VFQ79_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU0139435.1"
                     /translation="MRLLGPTEHPRLNEAVGLVFLFASVFLTLSLVSFHPQDPSWNTA
                     TSSVKPQNLIGYPGAHISDFLLQVFGIPAFLLPVLLVVLSWRWLLSRRIEAPFVKVAG
                     AALFLTSAAGLLSLGPDWRMFEDTVAPGGLAGTLLAAYLLDNLNQTGAILVAGMSLVF
                     SLYFVSTFSMRHVARLLRGPVRAWRSLLGRWSAWRARHRALRAEKARRKAELKAAAKA
                     ERKARRQKPEPAAGSLHLADTPPWEEESSSRTHPEEPEEEVELEEHDIPIRPLEEPPA
                     VSTHASVAPPVEFAPAARPVVEHLPERKQYELPPTSLLNEPAARNPYDSQELKEIAAR
                     IKSKFEEFNVLGSVVQINPGPVVTTFEFKPEAGIKYSRITTLTEDLCLGLQAESILIE
                     RIPGKPTVGIEVPNSKREVISLRQVLESEEFHNSPSRLTISLGKDINGRIKVTALESM
                     PHLLIAGSTGSGKSVMLNSMIMSILYKSTPDEVRLIMVDPKRVELGIYEGIPHLLTPV
                     ITDAKKATNALRNAVLEMERRLKLLAAQGVRNIDQYNRKMKQLAEQPRKLFEDDGAEL
                     EDLRPLPFILIIIDELADLMMLERGAVEETVTRLAQMARAVGMHLVLATQRPSVDVIT
                     GLIKANFPARISFRVATRVDSRTILDVMGAEHLLGKGDMLILPPGSSRLVRVHGAYVS
                     ESETNRVVEFWKSQAQPEYDRTFLIAPPADDEEEEPSGLDDAEDPMYQDAVRVVLEMG
                     KASTSTLQRRLRLGYGRAARILDMMQRDGIIGPPDGSRPRDVLKRPDWLEEVENQTR"
     gene            4660..4848
                     /locus_tag="VFQ79_06995"
     CDS             4660..4848
                     /locus_tag="VFQ79_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139436.1"
                     /translation="MFLWRATRGYRLRPWKSPYLRWRIETYSGVPADRIGFREFWCFV
                     WQHRSDLLRFVRWAGRMR"
BASE COUNT          938 a         1461 c         1412 g         1042 t
ORIGIN      
        1 tctccgtcag aaactggtca aatcgtctca ggcgattcgg acgcgtgatg aattagaacg
       61 cgaaattgcc gaccgcagga cggggcgaca cagctaatac atgatctgca cctttctgga
      121 tgcaggtgtg ctcatcgccg ctgcgaattt ccgccagact ccagcagggc tgcgcgcgtt
      181 tcaggtattg gacgacccgt aaagagagtt tgaatctttc cgttctcgtg atcgaaaggg
      241 acctgaaggg ggaacaattc gatccggaaa tccatcatgt cgatcacgtc tggccattcg
      301 caaaaggcgg tagtaatacc accgataacc tgagagtggt taggaaaaag gacaatctgc
      361 gcaaaggttc gaagctgccc agactcaggg atttctaggg caccggaatc tctcccagat
      421 gccctcagtt tcgttatgtc tcacttccgc gggctaaccc ctctcccagc cccgccggcg
      481 cgtctggcgc caatctggcg cattttcctg cagtatgcgg cgggcaaatg ccttgtcgtc
      541 ttccgaagta tcggggtcgg cgagccagct ttcccactcc cgccggttcc tttcgtgtag
      601 cagctcgggc cggacattgt tccgatgcct gcccactctt cgggcagcgc cctgggcctc
      661 cagatccgct attgcgcggc gtacgttctg tttggttgca cagccgatca tatcgagcgc
      721 gttcagctcg gctacgatgt ccccagggct cagcggcacg gtggccggct ttccattgac
      781 ccacttgacc gccaggcgcc tttgctggcc aacggaatgc aacatcccgc aagcccacac
      841 gcgcacctgg aagtcctgat cgggacgcat cagaaaccgg agctgctcgc tggtgatgaa
      901 ctgccagcgt ccggcccgga tgccccggcg caaaagaaac tccggtgagc catcctcccg
      961 gaactggcgg cagtcgcagg actcgcaggc aggattgtgc tgtgcagctc catgtccgca
     1021 gacgcagggg cgcgcctggt gctgctggca gccgcactga gaacagccgc tttcgctgtc
     1081 gtgatcggcc gccggatgcg agcagtagca gtgctcggtc cggatttttc ccgccggctt
     1141 ctttggtact tttccttgcg gggctgttgc cccggattcg ctatgatcga atggcatatt
     1201 tgatctccag aaattttttc gaacacgctt ctggaatcgc gccgcgctgg tgtgacagaa
     1261 tggcagggac ggccagactg tcacacccgt ggcaaccgga ccgttaccct ttcggcgcga
     1321 ctcaacgtgc cccggaacgc tgtttggact tgggatgagc ttgggcagca ttcggggaaa
     1381 ggttaaccgg aggggatctt ccaaggggca taggcagtaa gtcgcgtttg cgataattgc
     1441 tgtctatgcc cctttatcag gtcatcaccc ttgccatcgt gcaggggttc acagagttcc
     1501 ttcccatcag cagttccgcc cacctggctc tggcgccatg gttggctggt tggaaggacc
     1561 aggggctcac gttcgatatc gcgctgcatt tcgggacgct ggcggccgtg gcgatctatt
     1621 tctttcgcga ctggcttcag atcaccgcaa gcggttttgg catcgattac ggaaacgatc
     1681 ccagcctgaa acagaatccg cgcctgttct ggctgttggt cgcggccacc attcctctgg
     1741 gcgtattcgg ccttctggcg aaggaatacg ccgaaacggt gtggcgcaca ccgtatgtca
     1801 tcggtacaat gctgatcctt gtcggcattc tgatgttagt tgccgaacat accgcccgct
     1861 tgaagaaaga catcgacaga atatcggcgg cggacgccat ggcgatcggc gtgtcgcagg
     1921 cgcttgccgt tgtgccggga acatcgcgta gcggcatcac catcaccgca ggcctgtttc
     1981 gtggaattac acggccggcg gccgcccgct tctcgtttct tctgtccacg cccgcgatcg
     2041 ccgctgccgc cctcagcgct tttaaagacc tgctggaaag tggacagccg attgacctca
     2101 tgccttttat cgtcggtatg tctgtcagcg ccttaaccgg aattttagtg attgcatttt
     2161 ttctccgttt tctccgcaac cacagcttga agttcttcat ttactacaga attgtttttg
     2221 gcataatagt gattgctctg gctgctttct tccgccaccc cgcgggatga ggctcttagg
     2281 tccgacggaa caccccaggc tgaatgaagc cgtaggtttg gtgtttttat tcgcaagcgt
     2341 gtttctcacg cttagcctgg tttcttttca cccgcaggat ccctcctgga acaccgccac
     2401 cagctctgtc aagccgcaga atcttatcgg ctatcccgga gcgcacattt ccgatttcct
     2461 tcttcaggta ttcggcatcc cggcatttct ccttccggtc ctgttagtgg tgctgtcgtg
     2521 gcgttggctg ctgtcccggc gcatcgaagc gccattcgtc aaagtcgccg gcgctgcgct
     2581 ctttctcact tccgccgccg gactattgtc gcttggcccg gattggagga tgttcgaaga
     2641 tacggtagcg cccggcgggc tggcaggaac gctgctcgcg gcctatctcc tcgacaacct
     2701 gaaccagacc ggagcgattc tggttgccgg aatgtcgctg gttttctcgt tgtattttgt
     2761 ttcgacattc tccatgcgcc atgtcgcccg cctgttgcgc ggaccggtcc gtgcctggcg
     2821 ctccctgtta ggacgctgga gtgcctggcg cgcccggcac cgtgctctta gggctgaaaa
     2881 agcccgccgg aaggccgaac tgaaggccgc cgccaaagct gaacggaaag cccggcggca
     2941 gaaacccgaa cctgccgctg gctcgctcca tctcgccgac actccacctt gggaagagga
     3001 atcgtccagc cggactcatc cggaagagcc ggaagaggag gtggaactgg aagagcacga
     3061 cataccgata cgcccgctgg aggagccgcc tgccgtttcc acacacgcca gcgtggcgcc
     3121 gccggttgaa ttcgcgccgg cggcaaggcc cgtggtggag catcttcccg aaagaaagca
     3181 atatgaattg cctcccacca gcttgctgaa cgagccggcg gcccgcaatc cgtacgacag
     3241 tcaggaactc aaagaaattg cggctcgcat caagtcgaaa tttgaggaat tcaacgttct
     3301 cggctcggtt gtgcagatca atccgggccc ggttgttacc acgttcgagt tcaagccgga
     3361 agcaggcatc aaatacagcc gcatcacgac tctgaccgag gacctttgcc ttggcctgca
     3421 ggcggagtcg attctcatcg agcggattcc gggcaagccc accgttggca tcgaagttcc
     3481 caactccaaa cgcgaagtga tcagcctccg ccaggttctc gaatcggagg agtttcacaa
     3541 ctcgccgtcg cgcctcacca tttcgctcgg aaaagacatc aacggccgca tcaaggtgac
     3601 agcgctcgaa tccatgccgc acctgctgat cgcaggctcc actggatcgg gcaagagtgt
     3661 gatgttgaat tccatgatca tgtcgattct ctacaaatcg acgccggacg aagtgcggct
     3721 gatcatggta gatcccaaac gggtggagct cggcatttac gagggcattc cacacctgct
     3781 cactccggtg atcaccgacg caaaaaaagc gacgaacgcg ctgcgcaatg ctgtgctcga
     3841 gatggaacgg cggttgaagc tgcttgcggc gcagggcgta cggaatatcg atcagtacaa
     3901 ccggaagatg aagcagttgg ccgaacagcc gcgcaaactg tttgaagacg atggcgccga
     3961 actggaggat ctgaggcccc tgccgttcat cctcatcatc atcgacgagc tggccgacct
     4021 gatgatgctg gagcggggcg ccgtcgaaga aacagttaca cggcttgcgc aaatggcccg
     4081 cgcggtcggc atgcacctgg tgcttgcgac gcagcggccc agcgtggacg tcatcaccgg
     4141 cctgatcaaa gcgaatttcc cggcccgcat cagtttccgc gtggcgacgc gtgtggattc
     4201 gcgcaccatt ctcgacgtga tgggcgcgga gcaccttctc ggaaagggag acatgctgat
     4261 tcttccgccg ggctcatcaa gacttgtgcg cgtacacggc gcttatgtat cggagtccga
     4321 aacgaatcgc gtcgtggagt tctggaagtc gcaggctcaa cccgagtatg acagaacgtt
     4381 cctgatcgct ccccctgcgg acgatgagga ggaagaacct tccggattgg atgacgcgga
     4441 ggacccgatg taccaggacg ccgtgcgtgt tgttctcgaa atggggaaag cctccacatc
     4501 gaccttgcag cgccgtctgc gcctgggcta tgggcgcgcc gcccggattc tcgatatgat
     4561 gcagcgtgac ggaatcatcg ggccgccgga cggcagccgc ccccgtgacg tgctgaaacg
     4621 gccggattgg ctcgaagagg tcgaaaacca gacacggtga tgtttctctg gcgcgcgaca
     4681 cgcggctacc ggctccgccc ctggaagagc ccctatctcc gctggagaat cgagacctat
     4741 tcgggagtgc ccgccgaccg catcggcttc cgcgagttct ggtgcttcgt gtggcaacac
     4801 cgctccgacc tgctccggtt cgtccgctgg gccggcagaa tgcgttgaat acg
//
LOCUS       DASTMK010000159         6251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_200131, whole genome shotgun sequence.
ACCESSION   DASTMK010000159 DASTMK010000000
VERSION     DASTMK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200131"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1869
                     /locus_tag="VFQ79_07000"
     CDS             115..1869
                     /locus_tag="VFQ79_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0139437.1"
                     /translation="MADTAAGVLIDTIHDWGVDVVFGMPGDGINGMIEALRVRQDKIR
                     FIQVRHEESAAFMACGYAKFAGKLGCCIATSGPGGIHLLNGLYDAKLDGQPVLALTGL
                     QFHDLIGTHTQQDVALDKLFMDVSVYNERIMGAAHVEPLANLACRTALAYRGVAHLTM
                     PVDLQEEKVESGDASKRNVPHHSSEIYARSARLPLPADLRHAADVLNAGRKIAILCGR
                     GALHATDELEHVAEILGAPIIKALLGKAAVPDDSPYTTGGIGLLGTKPSQEAIEECDT
                     LLMVGTSFPYIEFLPKPGQARCVQIELDPSRIGLRYPVEAGLVGDSKRSLQELIPLLR
                     RNENRQFLTKAQEGMREWWKVMDERASYPEMPMKPQVLARELGKRLSDTAIVTCDSGT
                     ITTWWARHIPVKRQQMHSLSGNLATMATGLPYAIAAQVAYPDRQVVAFTGDGGFTMLM
                     GELATCVKYDLPVKVIVVRNNSLGQIKWEQMVFLGNPEYVCDLQPIDFAAVARACGVT
                     GFTVDNPKNAGRVIEEALNTPGPVVVDAIVDPHEPPMPPKTTVEQATKFAQSLLRGTP
                     HAGKIALTVAKNKVRELV"
     gene            2065..2619
                     /locus_tag="VFQ79_07005"
     CDS             2065..2619
                     /locus_tag="VFQ79_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0139438.1"
                     /translation="MRILKVLSAISVFCVTPLFAQADYAELAGFGGFVSGIGTHGTVG
                     GSLGYSTSRHIMPILEFSYIPLGSDEFAFGDRFSRTLVRRDSRAYEFNGGLHIRFPMS
                     RWRAVPYGAFGLGFVRSSVEIDSASFLQQYSNRLSDTDFAASFGGGVRYPIAGGFGLR
                     PEIKVFTTGSTWVRFSAGIYYEFR"
     gene            complement(2616..3518)
                     /locus_tag="VFQ79_07010"
     CDS             complement(2616..3518)
                     /locus_tag="VFQ79_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0139439.1"
                     /translation="MLFVTFRAADGAVEPGIVRDGKVMGLSFAGFADLIGIIRNGEQA
                     RAAVESAASNPPADSVHDVSDVQLLAPIPRPPKFICVGLNYRDHAREARMEIPKVPTI
                     FCKFPTCVIGPGEPIVLPANSAKPDYEAEFAFVVGKGGRHLPRESWQEHVFGYTNVND
                     VSARDFQMATTQWIMGKTFDTFGPMGPYLVSADEISDPHALDISMTINGERLQHSNTR
                     ELIFRIPELIEYLSSVFTLEPGDVVATGTPAGVGFAHKPPRWLRDGDDVVVRIEGLGE
                     LRNPVVAEKRIQTERHKGLRHLSN"
     gene            complement(3547..5427)
                     /locus_tag="VFQ79_07015"
     CDS             complement(3547..5427)
                     /locus_tag="VFQ79_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139440.1"
                     /translation="MAGESEKGSSAPRIRSMLPDVWQLVKPRWKALAVGLVLIAINRV
                     AGLVLPYSTKFLVDDVIGQRRFDLLQPIVFAVLGATAIQGTTSFSLTQLLAKAAQRLI
                     ADLRRKVQAHVGRLPVSYYDRNKTGTLVSRIMSDVEGIRNLVGTGLVEFAGGLITASV
                     ALVVLLRINPLLTAIAVSMILVFVLVLRKAFKTIRPIFRERGKIYAEVTGRLTESLGG
                     VRVVKGYHAEAREAEVFSGGVERLLNNVLRSLTGTSWMSFSATLMMGVVSAVVMYMGA
                     REIFSGSLTLGEFVTFTAFLAFLVAPMFQIVSIGTQVTEALAGLERTKEVLNEHLEDD
                     DPNRIVSLDRIRGEVEFRNVSFGYEPGKTVLHDVSFVARPGTVTALVGPSGSGKSTII
                     GLVAAFHNPDSGSVRIDGTDLSRVSLPSYRVQLGVVLQETFLFDGTIRENVAFGRPDA
                     TEEEILNACRIARVDEFAEGFEKGYDTVVGERGVKLSGGQRQRVAIARAVLADPRILI
                     LDEATSSLDSESEAAIQKALAYLMQGRTTFVIAHRLSTIRRADQILVIESGRVVEQGT
                     HNSLYAARSRYYDLYTKQHGVESNLFLAPGEGAQADGPEQVAEEAAADARLEVLKAIR
                     PV"
     gene            5669..>6251
                     /locus_tag="VFQ79_07020"
     CDS             5669..>6251
                     /locus_tag="VFQ79_07020"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722061.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEU0139441.1"
                     /translation="MGQLSVDLSTRASDLKRALPREQRTGVSFPRYFTGRLEPGKTPY
                     DEVQWELRTASIGNDKGTVIFEQRDVEIPIDWSQTATNIVASKYFHGKIASPDRERSV
                     AQLVHRVVDTITDWGREGRYFKSDEDAGNFRDELAHLMLNQKACFNSPVWFNVGVKES
                     RGYGWYYDEATDSIRKLRNDLAKPQCSACFINSV"
BASE COUNT         1278 a         1861 c         1847 g         1265 t
ORIGIN      
        1 gtacctctac ggcgtcctgt ttaaaccgcc gcaaccggcg gcgggccact ttctctgtaa
       61 caccatcctc cggcatcgat ctaccaagtg ggaccgaaag aacggaggag atacatggcc
      121 gatacagccg ctggtgttct gatcgacacc atacacgatt ggggcgtcga tgtcgtgttc
      181 gggatgcctg gagacggcat caatggcatg attgaggcac tccgggttcg acaggacaag
      241 attcgcttta tccaggtgcg tcatgaagaa tccgccgcgt tcatggcctg cggatacgcc
      301 aagttcgccg ggaagctggg ctgctgcatc gccacctcgg ggcccggcgg cattcacctg
      361 ctgaacggac tttacgatgc gaaattggac ggccagcccg tgctggcgct caccgggctg
      421 caatttcacg atctcatcgg aacgcatacg caacaggatg tggcgttgga caagctgttc
      481 atggatgtga gcgtttataa cgagcgcatc atgggggcgg cacacgtaga gccgctggca
      541 aacctcgcct gccgcacggc gcttgcttac cgcggcgtgg cgcatcttac gatgccggtg
      601 gacctgcaag aggaaaaggt ggaatccggc gatgcttcca agcgcaacgt accgcatcat
      661 tcgagcgaga tttacgcccg gagcgcgcgc ctacctttgc cggcggacct tcgccacgca
      721 gcggatgtat tgaacgccgg ccgcaaaatc gccattctgt gcgggcgcgg cgctctacat
      781 gcgaccgatg agttggagca cgtggcggag attctgggcg cgcccatcat caaggcgctg
      841 ctcggcaaag ccgccgtgcc ggacgatagc ccttacacca ccgggggcat cggacttctc
      901 ggcaccaaac catcgcagga agcaatcgaa gaatgcgaca cgctgctgat ggtgggcacc
      961 tccttcccct atatcgagtt cctgcccaag ccagggcagg cgcgatgtgt acagattgag
     1021 ctcgatccga gccggatcgg gctacggtat ccggtggaag ccggtcttgt gggcgatagc
     1081 aaacgatcgt tacaggagtt gatccccttg ctccggcgca acgagaaccg gcagtttctg
     1141 acgaaagcac aggagggcat gcgggaatgg tggaaggtga tggacgagcg cgcgagctac
     1201 cctgaaatgc ccatgaagcc acaggtattg gcgcgcgagt taggcaagcg gctgtccgac
     1261 acggccattg tgacgtgcga ctcgggcacc atcaccacct ggtgggcccg ccacattccc
     1321 gtaaaaaggc agcaaatgca ttcgctgtcg ggaaaccttg ccaccatggc gacaggactg
     1381 ccgtatgcga ttgccgcgca ggttgcctat ccggacaggc aggtggtggc gtttaccggc
     1441 gacggcggat ttaccatgtt gatgggcgag ctggctacgt gcgtaaagta cgatcttccg
     1501 gtgaaggtca tcgtggtgcg gaataattcg ctgggacaaa tcaagtggga gcagatggtt
     1561 tttctcggaa accccgaata cgtctgcgac ctccagccca tcgactttgc cgccgtcgcc
     1621 cgcgcctgcg gtgtcacggg attcacggtc gacaacccga agaatgcggg tcgtgtgata
     1681 gaggaagccc tgaacactcc gggtccggtt gtagtcgacg ccattgtgga tccgcatgag
     1741 ccgccgatgc cgccgaagac cacggtggaa caggccacga agttcgctca atcgctgctc
     1801 cgcggcacgc cgcacgcagg caagatcgcg ctgacggtag ctaagaataa ggtgcgcgaa
     1861 ctggtatagg caacaggctc gctgcaccag acggcgggat gtaggggatt ccagtatact
     1921 ggaatttctt gcatggacgt cgtgggagcg agtctgctca tactcctcgg cgctgcgtta
     1981 gcgaatctgc tcgatacagc gttcgaactt acaccaaaga ttacgtcatg gattggacgt
     2041 tttctggatt gacctgtcag gttcatgaga atactaaaag ttctatcagc catcagtgtg
     2101 ttttgcgtca cgccgctgtt cgcccaggcg gattacgcgg aattagcggg ctttggagga
     2161 tttgtatccg gcatcggcac gcacggaacg gtgggaggca gcctcggtta ctccacgtcc
     2221 cgccatatca tgcccatcct ggagttctcg tacatcccgc tgggcagtga tgagttcgca
     2281 ttcggcgacc ggttcagccg gacgctggtt cgccgcgatt cgcgcgcgta cgaatttaac
     2341 ggcggattgc acatccgttt tcctatgtcg cggtggcgcg ccgttcctta tggagctttc
     2401 ggcctggggt tcgtacgctc ttcggtggaa atcgatagcg ccagcttttt acaacagtat
     2461 tcgaacaggc tcagcgatac cgacttcgcc gccagtttcg gcggtggcgt gcgatacccg
     2521 atcgccggcg gatttggcct ccgtccggag atcaaggtgt tcactaccgg atcgacatgg
     2581 gtgcggttct cggccggaat ctattacgag ttccgctaat tgctgagatg gcgcaatcct
     2641 ttatgacgtt ctgtttgtat tcgcttttct gcgactactg gattccgtaa ttcgccgagc
     2701 ccttcgatcc ggaccacgac atcgtcgcca tcgcgcagcc agcgcggcgg tttatgagcg
     2761 aagccgacac ccgcgggcgt tcccgtcgcc acgacgtcgc ccggttccag cgtgaagacg
     2821 ctggagagat attcgatcag ttcgggaata cggaagatga gctctcgcgt gttggagtgc
     2881 tgtagccgtt cgccgttaat cgtcatcgat atgtccagtg cgtgcggatc ggagatctca
     2941 tcggcgctca cgaggtaggg ccccatcggt ccgaacgtat cgaacgtctt gcccatgatc
     3001 cactgcgtgg tcgccatctg gaaatcgcgg gcgctgacgt cgtttacgtt tgtatagccg
     3061 aacacatgct cctgccagga ctccctgggg agatgccttc cgccctttcc gacgacgaaa
     3121 gcgaactcag cttcgtaatc cggcttggcg gaatttgcgg gcagcacaat cggctcgccc
     3181 gggccgatga cgcaggttgg aaatttacag aaaatcgtcg gcactttggg aatttccatc
     3241 cgtgcttctc ttgcgtggtc gcggtagttc aatccgacgc agatgaattt cggcggcctc
     3301 ggaatgggcg cgagcaactg gacgtccgat acatcgtgaa cggaatcggc gggcggattg
     3361 gaggcggcgg actcaaccgc cgcgcgcgcc tgctctccgt tccggatgat gccgatcaga
     3421 tcggcaaatc ccgcgaacga caaccccatg acttttccat cccggacaat acccggttcc
     3481 actgcgccgt ccgcggcgcg aaaagttacg aaaagcatat gtgattagaa attccctccg
     3541 gggacttcat accggccgga tggctttgag cacttccagc cttgcatctg ccgcggcctc
     3601 ttcggccacc tgctccgggc catccgcctg tgccccctcg cccggcgcca ggaacaggtt
     3661 cgattctacc ccgtgctgtt ttgtgtagag atcgtaatac cggcttcggg ccgcatacaa
     3721 cgagttgtgc gtgccctgct ccacgacccg gcccgattcg atgaccagga tctgatcggc
     3781 gcgacggatc gtagacaagc ggtgtgcgat cacgaacgtt gttcttcctt gcatcaggta
     3841 ggccagtgct ttctgaatgg ccgcttcaga ttcggaatcc aggctcgaag tcgcttcatc
     3901 cagaatcaga atgcgagggt cggccagaac cgcgcgcgca atcgctacgc gttgccgctg
     3961 cccgcccgaa agcttcacgc cgcgctcgcc caccacggtg tcgtaaccct tctcaaagcc
     4021 ctcggcaaat tcatcgacac gcgcgatccg gcacgcgttc agaatctctt cctccgtggc
     4081 atcgggtctg ccgaaggcga cattctcccg aatggtgccg tcgaacagga acgtctcctg
     4141 taacacgacg ccaagctgaa cgcgatacga cggcaggctg acccgcgaca ggtccgttcc
     4201 gtctatccga accgagccgg aatccgggtt gtggaacgcg gcaaccaggc cgatgatggt
     4261 cgacttgccc gagcccgacg gcccaaccag cgccgtcacc gtgcccggcc tcgccacgaa
     4321 agagacgtcg tggaggaccg tcttgcccgg ttcgtagccg aagctcacgt tgcggaattc
     4381 cacttccccg cggatacgat cgagcgagac tatgcggttc ggatcgtcgt cctccaggtg
     4441 ctcgttcaac acctctttgg tgcgctccaa tccggcgagc gcctcggtga cctgcgttcc
     4501 gatcgaaacg atctgaaaca tcggcgccac caggaacgcc agaaacgcgg tgaacgtgac
     4561 gaactcgccc agcgtgagcg atccggagaa gatctcccgg gcacccatgt acatcacgac
     4621 agcgctaacc acgcccatca tgagcgtggc tgaaaaactc atccaggagg tgccggtcag
     4681 cgagcgcagg acgttgttca ggagcctttc gacgccgcct gagaacactt ccgcctcgcg
     4741 ggcttcggcg tgatacccct tgaccacccg gaccccgccc agcgattccg tcagccgccc
     4801 cgtgacttcg gcatagatct ttccgcgttc gcggaagatg gggcggatgg ttttgaaggc
     4861 tttccgcagc accaggacaa aaaccagaat catcgagacg gcgatggccg tcagaagcgg
     4921 attgattcgc aacagcacga ccagcgccac cgaggcggta atcagtcctc cggcaaactc
     4981 taccaggccg gtacccacca gattccgtat cccttcgacg tcgctcataa tgcgcgatac
     5041 gagcgttccg gtcttattgc ggtcatagta ggagacgggc aggcgtccga cgtgtgcctg
     5101 cactttgcgg cgcaagtccg cgatcaggcg ttgcgcggct ttcgcaagca actgggtcag
     5161 ggagaatgaa gtggttccct gaatggctgt cgccccgagc accgcgaaga cgatcggctg
     5221 caacaggtca aaccgccgct gcccgattac gtcatcgacc aggaatttcg tggagtaggg
     5281 caggaccagg cctgccaccc ggttgatggc gatcaggaca agtcccaccg ccagcgcctt
     5341 ccaccgcggt ttgacaagct gccaaacatc gggcagcatg gaccggattc gcggcgcgct
     5401 cgagcccttc tctgattccc ctgccactta agttaatcct aaaggatttg gatgcgcctg
     5461 cgcctgccgc cggcgctggg tgcctgcgga cttgctcctg ttttttcacg tcaccgtaac
     5521 acactgtact attggtagat aaagtccttg accagccact atatattggg ccatacttac
     5581 gaacgttcgc tattatagtg gttgatccgc tagagcccct ggatggctgg taacaagcga
     5641 aaatcctaaa tggaggtaaa caaagagaat gggccaattg agcgtcgatc tcagcactcg
     5701 cgcgtccgat ctgaagcgcg ccctgccgcg ggaacaacga accggtgtgt cgtttccccg
     5761 ttattttacc ggccggctgg aacccggcaa aaccccctat gacgaagtgc agtgggaact
     5821 gcggaccgcg tctatcggga acgataaggg cacagtcatc ttcgaacagc gcgatgtcga
     5881 gatccctatc gattggtcgc aaaccgcgac gaacatcgta gccagcaagt atttccatgg
     5941 caagatcgcc tcccccgacc gtgagcgtag tgtcgcccaa ctggtgcatc gcgtggtcga
     6001 caccataacg gattggggca gggaagggcg atatttcaag tcggacgagg acgcggggaa
     6061 tttccgcgat gagctggcgc acctgatgct gaaccagaaa gcctgtttca attcgcccgt
     6121 ctggttcaat gtcggggtga aggaatcgcg cggctacggc tggtattacg acgaagccac
     6181 cgatagtatc cgaaagctcc ggaacgatct tgccaagccg cagtgttcgg cctgcttcat
     6241 caattcggtg a
//
LOCUS       DASTMK010000160         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_135832, whole genome shotgun sequence.
ACCESSION   DASTMK010000160 DASTMK010000000
VERSION     DASTMK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135832"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /gene="msrB"
                     /locus_tag="VFQ79_07025"
     CDS             <1..245
                     /gene="msrB"
                     /locus_tag="VFQ79_07025"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU0139442.1"
                     /translation="TSETKFDSGSGWPSFWAPAADENVGTEVDRSHGMTRTEVHCSRC
                     GAHLGHIFEDGPAPTHLRYCINSAALDFEQAKKAGE"
     gene            274..1245
                     /locus_tag="VFQ79_07030"
     CDS             274..1245
                     /locus_tag="VFQ79_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0139443.1"
                     /translation="MERKQLGDSDMQLTRIGVGAWAMGGGGWAFAWGPQDDDQSIAAI
                     RAALDAGVNWIDTAAVYGLGHSEEIVARAVEGRAERPYVFTKCERVWNERREISGSLK
                     ADSVRRECEDSLRRLKLDYIDLYQIHWPDPDEDIEEGWAEMAKLKQEGKVRYIGLSNF
                     NVPQMERAMKIAPITSLQPPYSLISRDAERDLLPFAAQNNIGVIVYSPMQSGLLSGAM
                     TRERVASFPPDDFRRRKPQFQEPQLSRNLQLAGKLGEIGRRHGRSAGEVAIAWTLRHP
                     AVTAAIVGLRSAEQFEGVKGALEFRLSAEEIQEIEDFQTRLNEPVRV"
     gene            complement(1270..1482)
                     /locus_tag="VFQ79_07035"
     CDS             complement(1270..1482)
                     /locus_tag="VFQ79_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139444.1"
                     /translation="MTTLKQRLEIGAAILDRMERRRSEQNSPALGSGIEKLIVDRELR
                     ELEVDLQPERTAGMVLARLRRRRTAQ"
     gene            complement(1539..>2507)
                     /locus_tag="VFQ79_07040"
     CDS             complement(1539..>2507)
                     /locus_tag="VFQ79_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0139445.1"
                     /translation="PGPWSTKLGHPTIEPAAFLEGTRKYREYFWKELMGRFDDPMLAP
                     NPRTRKIYDTEKWIGYEVVLDVWPDVFAWGVLLVPKNIGPGEKRPVVVCQHGRNGVPK
                     DVIEVEGNAYNRFAARLADRGFVVFAPHNLYRGEDRYRWLSRKANGVKASLFSFIIGQ
                     HDQLLRWLETLPFVDGSRMGFYGLSYGGETAVRVPAVLEKYALSICSGDFNNWTRKVA
                     ATDEPFSFMYTIEWEMPYFNLGSTFDYSEMTYLIFPRPFMVERGHHDGVGRDRWVAYE
                     YAKTQYLYTQFAMPERTEIEYFNGGHAINGQGTFRFLERHLGWTPR"
BASE COUNT          502 a          744 c          755 g          506 t
ORIGIN      
        1 ttacttcgga gacgaaattc gattccggca gcggttggcc cagtttctgg gcgccggccg
       61 cggacgagaa cgtcggcacc gaagtggacc ggtctcacgg catgacacgc accgaggtgc
      121 attgcagccg gtgtggagcc catctcggtc atatcttcga ggacggaccc gcgcctacgc
      181 acctgcggta ttgcatcaac tccgccgccc tcgacttcga acaggcaaag aaagccggcg
      241 aataactctc gcgcagttgt tataacgaaa gggatggaac ggaaacaact aggcgatagc
      301 gacatgcagt tgacgcgcat cggagtcggc gcgtgggcga tgggtggcgg cggctgggcc
      361 tttgcctggg ggccgcagga cgacgaccaa tccattgccg caattcgagc cgctttggat
      421 gccggggtca actggatcga caccgcggcg gtttacggat tgggccattc cgaagagatc
      481 gtggctcgcg cggtcgaggg ccgtgcggag cgcccgtatg tctttacgaa atgcgagcgc
      541 gtctggaatg agcgccgcga gatcagcggc agccttaaag cggactccgt gcggcgcgaa
      601 tgcgaagaca gcctgcgccg cctgaagctc gattacatcg atctctatca gattcactgg
      661 cccgatccgg acgaggatat cgaagaaggc tgggccgaaa tggcgaagct gaagcaggaa
      721 gggaaggtac gctacatcgg cctgtcaaat ttcaacgtcc cgcagatgga gcgggcgatg
      781 aagatcgcac ccattacgtc tcttcagccg ccttactcgc tgatttccag ggacgccgaa
      841 cgggatctgc tgccgtttgc cgcccaaaac aatatcggcg taattgttta ctctcccatg
      901 cagtcgggtc tgctctccgg cgccatgaca cgggagcgcg tcgcgtcgtt tccaccggac
      961 gatttccgcc gccgcaagcc gcagtttcag gagccgcagt taagccgcaa ccttcagctt
     1021 gcgggcaaat taggagagat tggcaggcgt catggccgtt ccgccggcga ggtggcaatc
     1081 gcctggaccc tgagacaccc ggcagtgacc gccgccattg tgggacttcg atccgcggag
     1141 caatttgaag gagtcaaagg ggcgctggag tttcgtttga gtgcggaaga gatccaggaa
     1201 atcgaggact tccaaaccag gctgaacgag ccggtgcggg tctgaacgga caccgctccg
     1261 gttcctctgc tattgtgctg tccgccgccg ccgtaggcgc gccagaacca taccggcggt
     1321 ccgctccggc tggaggtcga cttcgagttc gcgcaattcg cgatcgacga tcagcttttc
     1381 gattccggat cccagcgccg gtgaattctg ctcgcttctg cggcgttcca tccggtcgag
     1441 aatcgctgct ccgatctcca acctttgctt cagggtagtc attaccactc tctcccttcg
     1501 tttctaaacg cgcaaacgga gggcagaacc tgtcatcgct atcggggagt ccatcccagg
     1561 tggcgctcca ggaaccggaa tgtcccctgc ccgttgatgg catgcccgcc gttgaaatac
     1621 tcgatttcgg tccgttcggg cattgcgaac tgcgtgtaga gatattgcgt cttcgcatac
     1681 tcgtaggcaa cccagcggtc gcgccccaca ccatcgtggt ggccgcgctc caccatgaac
     1741 gggcgaggaa aaatcaggta cgtcatctcg gagtagtcga aagtgctgcc caggttaaag
     1801 taaggcatct cccactcgat ggtgtacatg aagctgaacg gctcgtctgt ggccgctacc
     1861 ttgcgggtcc aattgttgaa gtcgccggag cagatggaga gcgcgtactt ttccagtacc
     1921 gccggtactc gcaccgccgt ttcgccgcca tagctcaggc cgtaaaagcc catccttgag
     1981 ccatccacaa acggcagggt ttcgagccac cgcagaagct gatcgtgctg gccgatgata
     2041 aaggagaata gcgacgcttt cacgccgttc gctttccggc tcaaccagcg gtagcgatct
     2101 tcgccgcggt agagattgtg cggagcgaag acgacgaatc cacggtccgc cagccgggcg
     2161 gcgaagcgat tataggcgtt gccttctact tcgattacgt ctttcggtac accattgcgc
     2221 ccgtgctggc aaaccaccac cgggcgtttt tctcccggcc cgatgttttt cggcaccagc
     2281 agcactcccc aggcgaagac atccggccag acgtcaagca ctacctcata gccgatccac
     2341 ttttccgtgt catagatctt gcgcgtcctg gggttcggcg caagcatcgg atcgtcgaac
     2401 cggcccatca gttctttcca gaaatactca cgatattttc tggtgccttc gaggaacgcg
     2461 gcgggttcga tggtgggatg gccgagtttg gtagaccagg gtccggg
//
LOCUS       DASTMK010000161         5463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_200176, whole genome shotgun sequence.
ACCESSION   DASTMK010000161 DASTMK010000000
VERSION     DASTMK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200176"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFQ79_07045"
     CDS             complement(<1..582)
                     /locus_tag="VFQ79_07045"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0139446.1"
                     /translation="MTLHLGLKVAAVLYLAVTIVNSQEPSGGISGSVTDPSGATVPRA
                     RVIVKHKQTGQSQVRTTLESGFFTFPALPAGDYELLAEADGFAPLRVPQVKVEIDRTI
                     RLSLELAITGSERVEVSGVAETVDHDSNTLGQTVTERQITDLPLNGRNFTQLGLLQAG
                     VVPLTNGLISAGGSIKATQAFAVNGQRPESNNYL"
     gene            676..1842
                     /locus_tag="VFQ79_07050"
     CDS             676..1842
                     /locus_tag="VFQ79_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0139447.1"
                     /translation="MPITRRHLLQLAAATAASAGPSPAITKIEVFPTPYPVGARFKFL
                     PKPERPSVLVKITAEDGAIGWGQSVPVPTWSYETTESVTLTLRNYIAPVLIGRDPFDI
                     AGAHALMNRAISPSFSTGMPIAKAGIDLALHDLTARIRGQNVAERWGRKTPEKLTLSW
                     TINPQTLDETAKLVEEGKRHGYKHFNVKVAPDPKFDIEMCRIVRKMAPDAFFWADANG
                     GYDLATALAVAPKLADIGVDVLEQPIAANRLTGYREIRKQRALPILMDEGVVSSVELI
                     EFIRLGLLDGVAMKPARTAGLWDARRQIEIVQDAGLLFLGSGLTDPDIALAAAIHLYG
                     AYNLKFPAALNGPQFLTGSFLEKPLVVSGGDIEIPRGSGFGVQIDEKRIRSMQN"
     gene            1875..3149
                     /locus_tag="VFQ79_07055"
     CDS             1875..3149
                     /locus_tag="VFQ79_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0139448.1"
                     /translation="MKRLLLFLAAVSMAYAQTNYDLLLKGGHVIDPKNNLDALTDVAI
                     RDGKIAAIEPSIEATRAKKTIQVNGLYVTPGLVDIHVHLFTTTGIPDAWAGDNSIRPD
                     DFSFRTGVTTMVDAGSSGWRNFETFRHTVIDRARTRVFAMINIAGFGMLTDIVEQPPT
                     EMRPEEVARLARKHKDVVVGIKTAHYQGPEWVSVERAVQAGESAGIPIMVDFGYFLKE
                     RPYWQLVTEKLRPGDISTHAFRGPAPWINDSGKVYSYLYEARKRGVKFDVGHGGGSLV
                     FRNAVPAIKNEFYPDSISTDLHTGSMNGGMMDMPTTMSKFLVMGMPLKEVVLRSTWNP
                     AQMIHHPEVGHLTTGSPADVAVWSLLDGNFGYVDASGGRLEGRQRLHCEMTLKDGRIA
                     WDWSGRAATDYRQLPADYGVRDVEFIVPPPKP"
     gene            complement(3156..4190)
                     /locus_tag="VFQ79_07060"
     CDS             complement(3156..4190)
                     /locus_tag="VFQ79_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEU0139449.1"
                     /translation="MKDRPLRTTVIGSYPFPGWLEFAAAHLDRFGPADLAEMQKDAVI
                     AAVHDQVAAGLDVVTDGEQTRLDFNLSFYGYLEGIDLEAAPERRFGPPAHDQRGKHQI
                     KGTLEAPRGLGVVAEYQRLHGVATPGPVLKASIPGPYTLSGRIQPGGPYKDRWAVTEA
                     LLPLVRKELDALVAAGCREITVDEPSMSCYAYREDPRRFVDIFARTVESVRGKCRLST
                     HLCFGNYKARAVGPRRYAPMFPAFLDLPVDEIHLEMASREFAEIEMIAEIAKRCDVAV
                     GIVDVKSYYIEPPEEIAARVRLCLRHAPAGKLSFAPDCGLSQTARWAAFAKLRNMVEG
                     VRLVRSELGV"
     gene            complement(4187..4966)
                     /locus_tag="VFQ79_07065"
     CDS             complement(4187..4966)
                     /locus_tag="VFQ79_07065"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0139450.1"
                     /translation="MKLAGKVCLITGSTGIAAATARLSVEEGARVFLAGIDETSCASL
                     SGEIGAAHHVADLTIPAAADEAIERCLERFGRLDAVYNVAGISGRRFGDGPLHECTEE
                     GWDVTLTANLKTTFLVSRAALRHFLKAEPAASGLRGTILNMTSVLAFSPEPRHFAAHA
                     YAASKGAIVSLTKAMAAYYASHQIRVNAIAPGLVRTPMSKRAQENPEILQYMETKQPL
                     AGGILDAEDVAKASVFLLSDESRHITGEIVTVDGGWHVSSI"
     gene            complement(4963..>5463)
                     /locus_tag="VFQ79_07070"
     CDS             complement(4963..>5463)
                     /locus_tag="VFQ79_07070"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HEU0139451.1"
                     /translation="PLYLEKYEGYAGEILKGFRFGPHDAFLIISTSGLRPVIVEMAAG
                     VKARGLPVIALVSRQHSDTAPPGHSSGKKLADLADIVIDNQCPPGDCVVELEGLDWRT
                     GPASTVTGAMIINMLRCRTAELLVERGHKPELLPSHQFVGNTSAEEQLERFYEAYRKS
                     LAHLYK"
BASE COUNT         1082 a         1610 c         1717 g         1054 t
ORIGIN      
        1 cagatagttg ttcgactcgg gacgctgccc gttcaccgcg aatgcctgcg tcgctttgat
       61 ggagccgccg gcgctgataa ggccgtttgt caaaggcaca actccggctt gcagcaatcc
      121 aagttgagta aagttgcgcc cgttcagcgg gagatccgtt atttgcctct cggtgacggt
      181 ctggcccagc gtattcgaat cgtgatcgac cgtttccgcc actccggaca cctcaacgcg
      241 ctcggagcct gtaatggcca gttcgagcga aagccggatg gtgcggtcga tttctacctt
      301 cacctgcggc actcgcaatg gtgcaaagcc atccgcttcg gccaggagtt cgtagtcgcc
      361 ggccgggagc gcggggaaag tgaaaaaccc cgattcgaga gtggttcgaa cctggctttg
      421 ccctgtttgc ttgtgtttta cgatcacgcg ggcgcgtggc accgtggcac ccgatggatc
      481 ggtaaccgag ccgctgatac cgccggacgg ctcctgtgag ttcacaatcg tgacggcaag
      541 gtacagaaca gccgcaacct tcaaccccag atggagagtc atcccagtgt gtgtctcggg
      601 ccagaatatc agcgcttgcg gctgcacaca acagaattta taggcgatac tcagggttcc
      661 agggccatac acgccatgcc gatcaccaga cgccatcttt tacaacttgc ggccgcgacc
      721 gctgccagcg ccggacccag cccggccatc acaaagatcg aggtattccc cacgccgtat
      781 cccgtcggcg cgcggttcaa gtttttgccg aaacccgagc gcccgtcggt gctcgtgaag
      841 atcacggccg aagacggcgc tatcgggtgg ggacaaagcg ttcccgttcc gacctggagc
      901 tatgagacca ccgagtccgt caccctgacg ctgcgcaact atatcgcgcc ggtgctgatt
      961 ggacgcgatc cgtttgacat cgccggtgcg cacgcgctga tgaaccgcgc catttcgccc
     1021 tcgttttcga cgggaatgcc gattgcgaag gcaggcatcg acctcgccct gcacgatctc
     1081 acggcaagga ttcggggtca gaacgtcgcc gagcgttggg ggcgcaaaac gccggagaag
     1141 ctgacgctga gctggaccat caatccgcaa acgctcgatg aaaccgccaa actggtggag
     1201 gaaggcaagc ggcacggcta caagcatttc aacgtgaagg ttgcgccgga ccccaagttc
     1261 gatatcgaga tgtgccgcat tgtccgcaaa atggccccgg atgccttctt ctgggcggat
     1321 gcgaatggag gctacgacct ggctacggcg cttgcggtgg cgccgaaact cgccgacatc
     1381 ggcgtcgatg tgctggagca gcccatcgcg gcgaatcgcc tgacggggta cagggagatt
     1441 cgcaagcagc gcgccttgcc gatcctgatg gacgagggcg tagtttcttc cgtcgagttg
     1501 atagagttca tccggttggg tttgctcgat ggtgtggcga tgaaacccgc gcgcaccgct
     1561 ggtttatggg atgcgcgccg gcagattgag atcgtgcagg atgccgggct gttgttcctg
     1621 ggcagcggtc tcaccgatcc ggatatcgcg ctggccgccg cgattcacct gtatggggct
     1681 tacaacctga aattccctgc cgcgctcaac ggaccacagt ttctgaccgg cagtttcctt
     1741 gaaaaaccgt tggtggtgag cggcggagac atcgaaattc cgcggggatc gggattcggt
     1801 gttcagatcg atgaaaagcg aattcggtcc atgcagaact gatgggactt cagcgccgtt
     1861 ctacaatgat ctggatgaag cgcttgctgc tgttccttgc ggccgtctcc atggcttacg
     1921 cacagacaaa ttacgacctg ctgctgaagg gcggccacgt tatcgatcca aagaacaacc
     1981 tcgatgcact gaccgatgtc gcgattcgcg acggaaaaat cgccgctatt gaaccgtcca
     2041 tcgaagccac cagggccaaa aaaacaattc aggtgaacgg gctctacgtg acgccgggcc
     2101 tggtggatat tcacgttcat ctattcacaa caacaggcat accggacgcc tgggccggtg
     2161 acaacagcat ccgtccggac gatttcagct ttcgcacggg tgttacgacg atggtggacg
     2221 cgggcagctc cggctggcgc aacttcgaga ctttccggca taccgtcatc gatcgcgcca
     2281 ggacgcgggt attcgccatg atcaacattg ccggcttcgg gatgttgacc gacatcgtgg
     2341 agcagcctcc aaccgagatg cgccccgagg aagtggcgcg gctggcccgc aagcataagg
     2401 acgtcgtcgt aggaataaag accgcacact accaggggcc cgaatgggtc tcggtggagc
     2461 gcgcggtaca ggcgggggag tcggccggga tacctatcat ggtggatttc ggttacttcc
     2521 tgaaggaacg gccgtactgg caactggtaa cggaaaagct ccggcccggc gacatcagca
     2581 cgcatgcctt ccgcggaccg gcgccatgga tcaatgatag cggtaaagtt tacagctatc
     2641 tctatgaggc gcggaagcgc ggtgtgaagt tcgacgtggg acacggggga ggcagtctcg
     2701 tattccgcaa cgcggttccc gccatcaaaa acgaatttta tccggactcg atttcaaccg
     2761 atctgcacac gggcagcatg aacggaggaa tgatggacat gccgactacc atgtcgaagt
     2821 ttctggtgat gggcatgccg ctgaaggaag tggtgctgcg ctcgacgtgg aatccggcgc
     2881 agatgattca tcatcccgag gtgggacatc ttactaccgg gtcccccgcg gacgtggcgg
     2941 tttggagcct gctggacggc aacttcggat atgtggatgc gagcggcggc cggttggaag
     3001 gccggcaacg cctccactgc gagatgacgt tgaaagacgg ccggatagcc tgggactgga
     3061 gcggacgcgc cgcaacggac tacaggcagt tgcccgcgga ctacggcgtg cgcgatgtcg
     3121 aattcatcgt gccgccgcca aagccgtgag gcgctttaca cgcccagttc cgaacgaacc
     3181 agccggacgc cttccaccat gttgcggagc tttgcgaagg ctgcccaccg ggcggtctgg
     3241 ctgagaccgc aatcgggcgc aaacgacagc ttgccggccg gagcatgtct caggcacagg
     3301 cgcacgcgcg ctgcgatctc ctctggcggt tcgatgtaat agcttttcac gtccacaatg
     3361 ccaacggcca catcgcagcg cttcgcgatt tccgcgatca tctcgatctc ggcgaattcg
     3421 cggctcgcca tctccagatg gatttcgtca acgggcaaat cgagaaaggc cgggaacatc
     3481 ggcgcgtagc ggcgcggccc caccgcgcgc gccttgtaat tgccgaagca caggtgcgtg
     3541 gacaggcggc atttgcccct gacggattcg accgtccgcg cgaaaatgtc gacaaaccgg
     3601 cgcgggtctt cgcgatacgc atagcagctc atcgacggct cgtcgaccgt gatttcgcgg
     3661 caacccgccg cgacgagcgc atcgagctct ttccggacga gagggagcag cgcctccgtg
     3721 acggcccagc ggtccttgta gggtccaccg ggttgaatac gtccgctgag agtgtacggg
     3781 ccgggtatac tcgccttcag gacgggcccc ggcgtcgcga cgccgtgcag gcgctggtat
     3841 tccgccacaa cacccaaccc gcgcggggct tcgagtgttc ccttgatctg gtgttttccc
     3901 cgctggtcat gcgcgggcgg accgaagcgg cgctccggcg cggcttcgag atcgattccc
     3961 tcgagatagc cgtaaaaaga gaggttgaaa tcaagccggg tctgctcgcc gtccgtgacg
     4021 acgtcgaggc ccgcggcgac ttggtcgtgc accgccgcaa tcaccgcgtc tttctgcatt
     4081 tcggccaggt cggccggccc gaaacggtcg agatgagcgg cggcgaattc cagccagccg
     4141 ggaaatggat agctgccaat gacggtggtg cgaagagggc gatctttcat attgagctca
     4201 catgccagcc gccatctacg gtcacgatct cgcccgtgat gtgccgggac tcgtcactca
     4261 atagaaagac ggatgctttg gcgacatctt cggcgtcgag gatccctccg gcgagcggct
     4321 gcttggtttc catgtattgc aggatttccg gattttcctg ggcgcgcttg ctcatgggcg
     4381 tgcgcaccag tcctggagcg attgcgttca ccctgatctg atggcttgca tagtaggcgg
     4441 ccatggcctt cgtcagagag acgatggcgc ctttgctggc ggcataggca tgcgcggcga
     4501 aatggcgggg ctcgggggaa aacgcaagaa cgctggtcat gttcaggatg gtgccgcgca
     4561 gaccggaagc ggcaggttcg gctttgagga aatgccggag agcggcgcga gagaccagaa
     4621 acgtcgtctt gagattggcg gtaagagtca catcccagcc ttcctctgta cattcatgca
     4681 gtggtccgtc gccgaagcgg cgcccgctga tgcctgcgac gttgtagacg gcatccagcc
     4741 ggccgaagcg ctccaggcaa cgctcgatag cttcatcggc cgccgccggt atcgtgagat
     4801 ccgcgacgtg atgggcggcg ccgatttcgc ccgacaggct cgcgcaggaa gtttcatcga
     4861 tgccggcgag aaaaacgcgc gcgccttcct cgaccgatag ccgcgcggtg gccgcggcaa
     4921 tgccggtcga cccggtgatg aggcagacct tgccggccag cttcatttgt ataaatgagc
     4981 gaggcttttc cggtacgcct cgtagaagcg ctccaattgc tcttccgcgc ttgtattgcc
     5041 gacgaactgg tggctgggaa gaagttcggg tttgtgaccg cgctctacca gtaattcggc
     5101 ggtacggcag cgaagcatgt tgataatcat cgcgccggtt acggtggatg ccggtcctgt
     5161 gcgccagtcc agcccctcca actccaccac acagtcgccg ggcgggcatt gattgtcgat
     5221 cacgatatcg gcgaggtccg caagcttctt gccggacgag tgtcccggcg gcgcggtatc
     5281 ggagtgctgc cgggaaacga gcgcgatgac gggcaagccg cgcgccttca caccggcggc
     5341 catttctaca attaccggcc tgagcccgct ggtggagatg atgaggaaag catcgtgcgg
     5401 accgaatcga aagcccttca gaatttcccc ggcgtagcct tcatattttt cgagataaag
     5461 cgg
//
LOCUS       DASTMK010000162        13182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_328019, whole genome shotgun sequence.
ACCESSION   DASTMK010000162 DASTMK010000000
VERSION     DASTMK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13182
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328019"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFQ79_07075"
     CDS             <1..412
                     /locus_tag="VFQ79_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0139452.1"
                     /translation="MCEQFGLDAFHGRNLEIDGYQVAGLGYSTPTPFDTPGEYSEEEM
                     ARRLNAFHPLDPLILICHCPPLGTTLDRIRAGAHAGSRAVLEFLETKQPAYFFCGHIH
                     EAEGTTEKIGQTTAINVGKRGFLLDFDKIKEVSL"
     gene            409..1543
                     /gene="prfB"
                     /locus_tag="VFQ79_07080"
     CDS             join(409..480,482..1543)
                     /gene="prfB"
                     /locus_tag="VFQ79_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_106861312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU0139453.1"
                     /translation="MTIEELEREFESLRQQGEAVRSWLDAPAKRSQLQKIEEQLNHPE
                     FWSQPEKSQKIMQERKRLEEVLSSDSLIGTATSDLETLFELSTEGEDVAAEISRELSA
                     FRKRLEELETGMLLSGDNDSRSAIVTIHPGAGGTESQDWAEMLLRMYLRWAERHNFEA
                     VVTDRLEGEGAGIKSVTFEVNGENIYGLLQGEVGVHRLVRISPFDTNARRHTSFASVF
                     VYPQIDDEIKIEIRPEDLRVDTFRASGAGGQHVNRTDSAIRMTHLPTGIVVQCQNERS
                     QHKNRASAMKQLKARLYEHEMEKKRAEDRKLEDTKLDIDFGSQIRSYVLAPYRLIKDH
                     RTKLSIGDVDRVLDGDIDELIHAFLVYRKTGRVSGDGRDELPE"
     gene            1550..2485
                     /locus_tag="VFQ79_07085"
     CDS             1550..2485
                     /locus_tag="VFQ79_07085"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU0139454.1"
                     /translation="MVDEEVLGAAASLIREGKLVAFPTETVYGLGANALDPEAVARIF
                     EVKGRPFTSPIIVHVQSVDMARNLVERWPDSAERLAKKFWPGPLTLVLRKHAKLPDVV
                     TGGLDTVGLRVPAHPVALELLRISGVPIAAPSANRFSELSPTTAEHVRQALGNSIEMI
                     VDGGPSTVGIESTVLSLCDDTPVLLRPGMISQPDIEAVIGPIEAMLGIVTSGAHPSPG
                     LHPRHYSPRTPLLLVVQGRVPKRGNGMYIGFEARSKAARTMILPLEPAAYAAELYQIL
                     HEADNGKWDWIAVECPPDTPEWAGILDRLNRAAQK"
     gene            2527..4923
                     /locus_tag="VFQ79_07090"
     CDS             2527..4923
                     /locus_tag="VFQ79_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139455.1"
                     /translation="MRWEYPLLLLLLVPALLHAQATATLTGRVLDPAGAPVESATAIV
                     ENALAGFQKEANTDAEGRFTIANLPFQSYVLTVRKPGFAMERQKVSLRTNVPVPVEVR
                     LALEQQVTQVEVSGTDPGALIEPEATGTRTELSATSFSRMPLPAGNRGLESVLLSFPG
                     FAANANGAIHPRGAHNQMTYVIDGMPVSDQLTGSFANAVDPSIVQNIELFTGNIPAEY
                     GNKISGVAVITTRSGSGTGRSFSGSAQIGAAQFGTLSQVSQFAGGTDRFGYFASFSAV
                     KSNRFLDQVSLDNLHNGGNSERGFARFDYHASPRHQFRMNLLAGRSSFELANLRSQHA
                     ARQDQRQLLRDFSASLGWLHTLNARATFDSTVSYRTSIAQLFPSAGDTPVTAAQARHL
                     STLVVANRISLLAGPHTLRAGVDVQHFPISENFSFGITDGNFNHPASQAFIPTLLRHD
                     LSRGGSLFYFSERAAGNMYSGFVQDNIRWRRFAFSLGFRYDAYRFLVDGNQLQPRIGV
                     AFHVPETGTVFRASYNRTYQTPPNENLLLSSSDQAAVLVPAGVRDALGGAYARIRPER
                     QNVYELGLQQAVGRKVSFNGSFYHKNSRDLQDNDNFFNTGIIFPTSLLQSRVNGAEAR
                     VALIPTRGFTASVSLTHYHVVVTPPFTGGLFLGSTAVDLLNSGPFVIDHDQTLAAHGM
                     VQYSFARGFWASTSIRYDSGLVSNPSDPAKVAADPDYFDLLPYVNLDSNIPRVRPRTI
                     VDAAVGYEKRSNERTIWDVSVQLSNITNATALYNFQSIFVGTRLVQPRTAGVKLRFFF
                     "
     gene            4969..6357
                     /locus_tag="VFQ79_07095"
     CDS             4969..6357
                     /locus_tag="VFQ79_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0139456.1"
                     /translation="MVRALATAILFAVSLGAQTGSPLADRAVEYLQALIRIDSSNPPG
                     NESRVAEYLQTLANEHGIENELLGGDPERLNFIARLRGTGRERPLLLMAHMDVVPAET
                     EQWNAPPFSGEVRDGFIYGRGALDAKNLLAAHFAVLVELKRRSVKLKRDIILLAEADE
                     EAGASGIRWLIENAYPKLDAEFAINEGGSAVDMSSGVRLFQIQTAEKIPTRLLLTAQG
                     TAGHGSMPRPDNAIVRLSRAITRLMDEEQPVRWNSTTRRYFRQIAELPGYRWLEKLLG
                     RLETDPVNAAAEIRPRAPELDAMLRTTISPTMLTSGVKINVIPNTAQAGLDVRRLPNE
                     TREEVISRFSRIINDRTVSISSAGEPEAPAAEPSSLSTALYRAMHFVFLNSHPKAAVA
                     PFMSRGATDAAFLREKGMAVYGAPLFLKEPGESRAHGNDERISLWNMRLGTEMLWNIL
                     IAVAVEDNAGAG"
     gene            complement(6304..7098)
                     /locus_tag="VFQ79_07100"
     CDS             complement(6304..7098)
                     /locus_tag="VFQ79_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0139457.1"
                     /translation="MPVPKRILSIHAHPDDAEILAGGTLALLSQRGHHLTIVTMTPGD
                     CGSHDYSSEEIARIRRVEAANAASLIGANYICAEFRDLSIFIDDPSRRRVVELLRKTQ
                     PDLVLTASPTDYMCDHEATSKLVTDACFAAPAPNYLTGDPNPAPPLAAIPHLYFMNPI
                     SGLDRDEVPVIPDFVVDVASTFQLKRDMLARHESQRKWLLEHHGMDDYLMTMEMWTRD
                     TGAGVGIEYGEGFRRYKGHPYPQTPLLEELAGAGVIFYRHSDEYVP"
     gene            7166..8971
                     /locus_tag="VFQ79_07105"
     CDS             7166..8971
                     /locus_tag="VFQ79_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139458.1"
                     /translation="MQEHSSVLPVGVTAEAVREQLERILESDMFAHSMRLSRFLRFTV
                     AEALEGRREHLKEHTIGVHVFDRTMDFDPRTDSIVRVEARRLRSKLERYYTSIGSRDP
                     IVILFRKGRYWPVIRLRESLVQDRRGTGFETVRTWKSIAVLPFRNLSGAPGNDYLSEG
                     VVQELVSALTKIDNLRVAARTSLSRFGGEERDAVRAGEELGADMILDGTISVQPGSIH
                     TSATLTSVRAGQVLWSDSFEAQSDDLLRVQEKIAAYIVRSIAATVSRELSAAQTTTSP
                     AILRQERIAAYMGRHLTEKALRSALACYEAAAAGAPADGRLNSGIAECWLLLALFGRT
                     ASANAAAKAGSFAAMALESDSLDPAANAVLSVLSGLPPSGASGRDRHLKICLSQSGRA
                     SARTLAVCAQSLACAGRLEEAIRTAHAANDFDPVEPYANAVLGELLWATGFPGMAISH
                     LRESIEIEPGCAPLHWALGLAASGTQDHVLALRHLEVAWELSDGAGYAGAGLAHALAM
                     DGQPSRAFELLHALEERAAECWFERSFVYLALGDVQKALNSLLKAIQSRSIWYPRIYV
                     WPQFAQLRSHPQVAAAVDTSEDSTLQASSAGGNGG"
     gene            complement(9000..9332)
                     /locus_tag="VFQ79_07110"
     CDS             complement(9000..9332)
                     /locus_tag="VFQ79_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0139459.1"
                     /translation="MSSRQVGDVTVIDAAGRITLGEGSSTFRDTVRDHIAKGNKKLLI
                     NLAEVSYIDSSGIGELVTAFTTVTNNGGQLKLLNLTHRVQDLLQITKLYTVFEVFSDE
                     AAGVRSFV"
     gene            complement(9370..9813)
                     /locus_tag="VFQ79_07115"
     CDS             complement(9370..9813)
                     /locus_tag="VFQ79_07115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0139460.1"
                     /translation="MPSNRSGENRSVETVLDSTLDSVDTAEQIVLRAATDVGFGEDEL
                     HKIGVAIREAMVNAVVHGNRYSAHKKVRLAVSEAPDRLTIRIQDEGEGFDVASLPDPL
                     AAENLLRQSGRGILLIRAFVDEFHVRHLESGGTELSLVLYRTRSS"
     gene            complement(9988..11370)
                     /locus_tag="VFQ79_07120"
     CDS             complement(9988..11370)
                     /locus_tag="VFQ79_07120"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HEU0139461.1"
                     /translation="MRFSILLLLAAMPGLSAERPTEDGYRGIWYYNQPSNDKYVYKYS
                     GGFVTYPQQHAPIAIYSKEANKTFFCYGGTAKGTHNRLLHMVSYFDHATGEVPRPVLL
                     LDKKTDDAHDNPTISIDGEGYIWIFSSAHGTSRPSYIHKSAKPYSIIDFELIQETNFS
                     YPQPWYVPGRGFLFLHTRYQKAEKGRSPGRALYWMVSPDGREWSSPRRLAFALQGHYQ
                     VSWSGGARVGTAFNIHPPPLGLNQRTNLYYLETRDFGDTWVTAGGETVDIPVTTEQNP
                     ALVRDYRSQGLLVYLKDVQYDSRGRPVILYMTSKGYESGPENGPRMFRTARWTSKNWD
                     FQPVAASDHNYDYGSIYLESDGTWRLIAPTGAGPQPHGTGGEIEMWTSGDEGKSWKMV
                     RKLTQGSRYNHTYVRRPLHAHPDFYAIWADGNALAPSDSSLYFADRNGGVWQLPTQMK
                     GAFAKPEKVR"
     gene            11390..11779
                     /locus_tag="VFQ79_07125"
     CDS             11390..11779
                     /locus_tag="VFQ79_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU0139462.1"
                     /translation="MSTETFRRHCLSFPHATEQVQWGDNLVFKVGGKLFAITSLEPGG
                     HFASLKCSPERFHELVERPGIVPAPYLARAYWIALEREDAAGHAELLELLREAYEIVL
                     AGLPKKIRESLGTTNANQPKRSRKRTA"
     gene            complement(11793..12125)
                     /locus_tag="VFQ79_07130"
     CDS             complement(11793..12125)
                     /locus_tag="VFQ79_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU0139463.1"
                     /translation="MSIGEPSKLLRIHIGEQDVFDGKPLYEAIVAKCQETGIAGATVI
                     PGIEGYGDSPPGGGHPLREERPVLISIVDTAENLALLVPEVEQMIGKGLIAMSDVKVI
                     RVEKSRRK"
     gene            complement(12112..12504)
                     /locus_tag="VFQ79_07135"
     CDS             complement(12112..12504)
                     /locus_tag="VFQ79_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU0139464.1"
                     /translation="MTETEVTRHPQERRQGRAKMLRIFLGEADQWQGEPLYEAIVKKL
                     QTLQVAGATVYRGILGYGAKTGPDSEQSRRLSKDRPIMISTIDTALRIAEAATAIEEM
                     LEDGLIVVSDVDIIRIIHNHPEISNVDR"
     gene            complement(12528..>13182)
                     /locus_tag="VFQ79_07140"
     CDS             complement(12528..>13182)
                     /locus_tag="VFQ79_07140"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0139465.1"
                     /translation="GGWVNGTKESMVRNFCLPYLEKGFVVANVEYRLAKAAPAPAAVE
                     DVLKAANWFRDNARRYNVDRDRIVVTGGSAGGHLALMVGMVPKSARLGKPAKVAAVVN
                     FYGITDVGDQLEGPNMRKYAVTWVPEQEARLVLARRVSPASYVRKDLPAILTIHGDAD
                     ETVPYEHGVNLTRELRDEGADAELIPIAGGKHGFSQEQLAEIYPRIFAFLERRGILK"
BASE COUNT         2677 a         3958 c         3865 g         2682 t
ORIGIN      
        1 catgtgcgag cagttcgggc tggacgcgtt ccatggccgg aacctcgaaa tcgacggcta
       61 tcaggtagcc ggcctgggct actccactcc gacaccgttc gacacccccg gcgaatacag
      121 cgaggaagaa atggcgcgca ggctgaacgc gttccatcct ctcgatccgt taattctgat
      181 ctgccactgc cccccgctgg gaaccacgct cgacagaata cgggcgggcg cgcacgccgg
      241 aagccgcgcc gtgctcgagt tcctcgaaac gaagcagccg gcgtattttt tctgcgggca
      301 tattcacgag gcggagggca ccaccgaaaa gattggtcaa acgaccgcaa tcaacgtggg
      361 aaagcgtgga tttttacttg attttgataa gattaaagaa gtaagcctat gacaatagaa
      421 gaacttgaaa gggagtttga atcgctccgc cagcagggtg aagctgtgcg gagctggctt
      481 tgacgctccc gccaaaaggt cccagttaca gaaaatcgaa gaacaattga accatcctga
      541 attctggtct cagcccgaga agagccagaa gatcatgcag gaaaggaagc gcctggaaga
      601 agtgctttca agcgacagcc tgattggaac ggcgacctcc gacctcgaaa cgttgttcga
      661 gttgtcgacc gaaggcgaag atgtagccgc ggaaatttcc cgggagctga gcgcattccg
      721 gaagcggttg gaagagctcg aaaccggcat gctgctgagc ggggacaacg attcgcgcag
      781 cgccatcgtg acgattcatc ccggagccgg cggcaccgaa agtcaggact gggcggagat
      841 gctcctgcgc atgtatctgc gctgggcaga gcgacacaat ttcgaagcgg tggtaacgga
      901 ccggctggag ggcgaagggg cgggcatcaa atcagtcacc ttcgaagtga acggtgaaaa
      961 tatttacggt ctgcttcagg gtgaggtcgg agtacaccgg ttggtgcgaa tttcaccgtt
     1021 cgataccaat gcgcggcggc acacttcgtt tgcctccgtt tttgtttacc cgcagattga
     1081 cgatgaaatc aagatcgaga tccgtccgga agatctccgc gtggacacgt tccgggcaag
     1141 cggcgcgggc ggtcaacacg tcaatcgcac agactcggcc atccggatga cgcacctgcc
     1201 cacgggcatc gtcgtgcaat gccagaacga acgctcgcag cacaagaacc gcgcgagtgc
     1261 gatgaagcag ctcaaggcgc gcctttacga acacgagatg gaaaagaagc gtgccgagga
     1321 ccggaaactc gaagatacca agctcgacat cgatttcggc agccagatcc gcagctatgt
     1381 gctcgcaccg taccgcctga tcaaggacca ccgcaccaaa ctgtctatcg gagatgtcga
     1441 tcgcgttctg gatggagaca tcgacgagct tatccacgcc ttccttgtgt accggaagac
     1501 cggcagagtg tccggagatg gccgcgacga gttgccggag tagcagggca tggtggatga
     1561 ggaggtgctg ggagccgcag cctcactgat ccgcgaaggc aagctggttg catttcccac
     1621 cgaaaccgtt tacgggcttg gcgcgaacgc cctcgatccc gaagcggtgg cgcgcatctt
     1681 cgaggtgaag ggccggccct tcaccagccc gatcatcgtc cacgtccaat ccgtcgacat
     1741 ggcgagaaat ctggtcgaac gatggccgga ttccgccgaa cgcctggcga agaagttctg
     1801 gcccgggccg ctcaccctcg tgctccgcaa acacgcgaag cttccggatg tggttacggg
     1861 gggattggat accgtaggac tgcgcgtgcc agctcacccg gttgctctcg aattgcttcg
     1921 catttccggc gtgccgatcg ctgcccccag cgccaaccgt ttctcggaac tctcgccaac
     1981 caccgccgag cacgtccgcc aggcgctcgg caactccatc gagatgatcg tggacggagg
     2041 gccgtccacc gtcggaatcg aatccactgt gttgtcgcta tgtgacgaca cgcctgtgct
     2101 gctacggccc ggaatgatct cgcagccgga tattgaagcg gtgatcggcc ctatcgaagc
     2161 aatgctggga attgtgacgt ccggagcaca tccgtcgcca ggacttcatc cccgccatta
     2221 cagcccgcgc acaccccttc tcctggtagt ccagggacgt gtcccgaagc gaggcaacgg
     2281 gatgtatatc ggctttgagg cgcgctcgaa ggccgcccga acgatgatcc tgccgcttga
     2341 accggcggct tacgcagcag agctctatca aatactccat gaggctgata acggcaaatg
     2401 ggactggatt gccgtggaat gtccgccaga taccccggaa tgggctggta tcctggaccg
     2461 gctcaaccgc gcagctcaaa aatagcttgt taaccgtagt ttaatcgtgt aagataatcc
     2521 tagtttatgc gctgggaata tcctctgcta ttgttgttac tggtccctgc ccttcttcat
     2581 gcacaggcga ccgccaccct cacgggccgt gttcttgatc cggccggagc gcccgtcgag
     2641 tcagcgacgg ccatcgttga aaatgctctt gccggcttcc agaaagaggc gaacaccgat
     2701 gccgaaggcc ggttcaccat cgccaatctg ccgtttcaga gttatgtgtt gacggtccgg
     2761 aaaccgggct tcgcaatgga gcggcagaag gtgagcttac gtaccaatgt tcccgtgccg
     2821 gtggaagtac ggctcgcgtt agagcaacag gtgactcaag tggaagtgag cggaaccgat
     2881 ccgggcgctc tgatcgaacc ggaagcgacg ggaaccagga ccgaactgag cgcaaccagt
     2941 ttttccagaa tgcccttgcc cgccggaaat cgcggtttag agtccgtgct tctgagtttt
     3001 ccggggttcg ccgcgaacgc caacggcgcc atccacccac gcggagccca caaccagatg
     3061 acctacgtta tcgacggaat gccggtgagc gatcaactca ccggatcgtt cgcgaatgcg
     3121 gtcgatccga gcattgtgca gaacatcgaa ctgttcacgg gcaacatccc ggcggagtat
     3181 ggcaataaga tctcgggtgt ggcggtcatt accacacgct ccggttccgg gaccggccgc
     3241 agcttttcgg gaagcgcaca gatcggcgcc gcgcaattcg gcacgctgtc gcaagtgtcg
     3301 cagttcgcgg gtgggacaga ccgcttcggc tacttcgcat ctttcagcgc cgtcaaaagc
     3361 aaccggtttc tggaccaggt ttcgctggat aacctgcaca acggcggcaa ctccgaacgc
     3421 ggcttcgcgc gattcgacta tcacgcctct ccgcgccatc agtttcgaat gaacctgctg
     3481 gcgggccgtt cttccttcga actggcgaat ctacgttcgc agcacgcggc ccgccaggac
     3541 cagcgccagt tactccggga tttttcagcc tccctgggat ggctgcatac cctcaacgcg
     3601 cgtgcgactt tcgattctac ggtgtcctac cgaacgtcca ttgcacaact gtttccgagt
     3661 gcgggcgata caccggtgac agccgcacag gcccggcacc tttccaccct tgtcgtcgcg
     3721 aaccgtatca gccttctggc ggggccgcac acgttacggg ccggcgtcga cgtacaacac
     3781 ttccccatca gtgagaattt cagtttcggt atcacggatg gcaacttcaa ccaccccgca
     3841 tcgcaagcat tcatccctac gcttctgcgc cacgatctga gccgtggcgg atcgctgttt
     3901 tacttttccg aacgtgcggc ggggaacatg tattccggat tcgtgcagga caacatccgc
     3961 tggagaaggt tcgccttctc acttgggttc cgatatgacg cttatcgctt cctggtggat
     4021 gggaaccagt tgcagccgag aatcggcgtt gcctttcacg ttcccgaaac cggcactgta
     4081 ttccgggcct cgtacaaccg gacctaccag acgccgccga atgagaatct gctgctttca
     4141 agctccgatc aagctgccgt cctggtaccg gcgggagtgc gggatgcctt gggcggggcc
     4201 tatgcccgca tccggccgga gaggcaaaac gtgtatgaat taggcctcca gcaggccgtc
     4261 ggacggaagg tgagcttcaa cgggtctttc taccacaaga attcgcgcga ccttcaagac
     4321 aacgacaact ttttcaacac gggcatcatc tttccgacgt cgctgctcca atcgcgcgtg
     4381 aacggcgctg aggcgcgcgt cgccttgatc cctacacgtg gattcactgc gtcggtcagt
     4441 ctcactcact accatgtggt ggttacgcct ccgtttacgg gcggcctgtt tctgggcagc
     4501 acggccgtgg atctgctcaa ctcgggaccc ttcgtgatcg atcacgacca gacgctcgcc
     4561 gcccacggca tggtgcaata tagctttgca cgcggtttct gggccagtac gtcgattcgt
     4621 tacgacagcg ggctggtatc gaatccttcc gatccggcaa aagtggcggc cgatccggac
     4681 tatttcgacc tgttacccta tgtgaacctg gattcgaaca ttccgcgcgt gagaccccgg
     4741 accattgtgg atgccgccgt gggatacgag aagcgcagca acgaacgcac catctgggac
     4801 gtttcggtcc agctatcgaa tatcacgaac gccaccgctc tgtataattt ccagtcgatt
     4861 ttcgtaggca ctcgcctggt gcaacctcga acggccggtg tgaagctccg gtttttcttt
     4921 tgatttcccc atccacgcgg tcgcggtaac tgctactatt catggaaagt ggttagggca
     4981 ctcgctacgg cgatcctttt cgccgtctct ctgggagcac aaaccggctc gccgttggcc
     5041 gatcgcgccg tggaatatct gcaggccctg atccgaatcg attccagtaa tccaccgggt
     5101 aacgaaagcc gggtcgccga atatctccaa accctggcaa atgaacacgg cattgagaac
     5161 gagcttctcg gcggcgatcc ggagcggctg aatttcatcg cgcgcttgcg cggcaccggc
     5221 agggagaggc cgctgctgct gatggctcac atggatgtcg tgcccgcgga aaccgagcaa
     5281 tggaatgcgc cgcccttttc cggcgaggtt cgggacggct tcatttacgg acgcggcgcc
     5341 ttggatgcca agaacctgct ggccgcccat tttgccgtat tggtggaatt gaagcgccgc
     5401 tcggtgaagt tgaagcgcga tatcattctg ctggcggaag cggacgagga ggcaggggcg
     5461 tccggaatcc ggtggctgat cgaaaacgcc tatccgaagc tcgatgccga gtttgcgatc
     5521 aatgagggcg gaagcgccgt ggacatgtct tccggcgtgc gcctgttcca gattcaaacg
     5581 gcggagaaga ttcccacacg cctgttgttg accgcgcagg gaacggccgg acacggttcc
     5641 atgccccgcc cggacaacgc catcgtccgc ttaagccggg ccatcacgcg tctgatggac
     5701 gaagaacagc cggtacgatg gaattctaca acgcgccggt acttccggca gatcgccgaa
     5761 ttgccggggt accgctggct ggagaagctg ttgggacggc tcgaaaccga tccggtaaac
     5821 gccgcggcag agatccgtcc gcgcgccccg gaactcgacg caatgctgcg taccaccatc
     5881 agcccgacca tgctgacgtc cggcgtgaag atcaacgtca ttcccaacac cgcccaggcc
     5941 gggctggatg tacggcgatt gccgaatgaa acgcgtgaag aagtgatcag ccggttttcg
     6001 cgcatcatca acgaccgcac ggtgagcatt tcatcggccg gagaaccgga ggcacccgcg
     6061 gccgagccga gttccctgtc aaccgcgctg taccgggcca tgcatttcgt gttcttgaac
     6121 tcgcatccca aggctgccgt agcacccttc atgagccgtg gagccaccga tgcagccttt
     6181 ctgcgggaga agggtatggc cgtctacgga gcgccgctct tcctgaaaga acccggagaa
     6241 agccgcgctc acggaaacga cgagcgaatc tcgctttgga acatgagact gggcaccgag
     6301 atgttatgga acatactcat cgctgtggcg gtagaagata acgccggcgc cggctagctc
     6361 ttcgagaagg ggcgtctgcg gataggggtg tcctttgtaa cgtctgaatc cctctccgta
     6421 ctcgatgcct acgccggctc cggtgtcgcg agtccacatt tccatggtca tcaggtagtc
     6481 gtccatgcca tgatgctcca gcagccattt ccgctggctt tcgtgccggg ccagcatgtc
     6541 ccgctttaat tgaaacgttg aggcgacgtc tactacgaaa tcggggataa cgggaacttc
     6601 gtcgcggtcc aggccgctga tgggattcat aaaatacagg tgcggaatag cggccagcgg
     6661 cggagcggga ttcggatcgc ccgtcaggta gttgggagca ggcgccgcga agcaggcatc
     6721 cgtcaccagc ttgctggtgg cttcgtggtc gcacatgtag tccgtggggg aagcggtcag
     6781 taccaggtcc ggctgcgttt tccgcagcaa ctcaaccacc ctgcggcgcg aggggtcgtc
     6841 gataaaaatc gaaagatcgc ggaattccgc acaaatatag tttgctccaa tcagggaagc
     6901 cgcattcgcc gcttccacgc ggcggattcg cgcaatctcc tcggaagaat agtcgtgcga
     6961 gccgcagtcg ccgggagtca tagtcacgat ggtgaggtga tgaccgcgtt gggacaggag
     7021 ggcgagagtg ccccctgcca gaatctccgc atcgtccggg tgtgcgtgaa tcgagagaat
     7081 gcgcttggga acaggcattc tgttattcta tcgctcttgc gcgaatcacc cttgtaaaga
     7141 cggcgactta tgagaggatt tatacatgca ggagcacagt tcggttctgc cggttggtgt
     7201 caccgccgaa gctgtgcgag agcaactgga gcgcattctg gaaagcgaca tgttcgcaca
     7261 ctccatgcga ctgagccggt tcctgcgatt cacggttgcc gaggccctgg aggggcgcag
     7321 ggaacatctg aaagaacaca ccatcggggt tcacgttttc gaccggacca tggatttcga
     7381 tccaagaacc gactccatcg tcagggtgga ggcacggcgt ctgcggtcga aactggaacg
     7441 ctactacact tccatcggga gcagggaccc cattgtgatt ctgttccgga agggccgtta
     7501 ttggccggtc atccgcctca gggaatcgct ggtacaggac cgtcgaggca ccggcttcga
     7561 gacggtcaga acctggaagt cgatcgcggt gttgcccttc aggaatctca gcggcgcgcc
     7621 cggcaacgat tatctttcag agggcgtggt tcaggaactc gttagcgccc tgacgaagat
     7681 cgataacctg cgcgtcgctg ccagaacctc tctatcgcgc tttggcggcg aggaacggga
     7741 cgccgtgcga gcgggcgagg aactcggggc cgacatgata ctggatggta ctatcagcgt
     7801 gcagcccgga tcgatccata ccagcgccac gctcacttct gttcgcgccg gccaggtgct
     7861 atggtcggat tctttcgaag cccagtcaga cgacctgctg cgcgttcagg aaaaaatcgc
     7921 cgcttatatc gtccgttcca tcgcggcaac ggtgagccgg gaattgtcgg ccgcacagac
     7981 taccacatcc ccggcgatct tgcggcagga gagaattgca gcctacatgg gccggcacct
     8041 tacggagaaa gcgttgcgaa gcgcgctcgc ctgctatgag gcagcggccg ccggagcgcc
     8101 ggcggacggg cggttgaact ccggtattgc cgaatgctgg ctactgttag ccctcttcgg
     8161 ccggacggct tccgccaatg ctgccgcgaa agccggctcc ttcgccgcaa tggcgctcga
     8221 atccgattcg ctcgatccgg ctgccaatgc ggttctgtcc gtactatcgg gtttgccgcc
     8281 gtccggcgcc tccggcaggg atcgccatct taagatctgc ctttcgcaaa gcggccgcgc
     8341 aagcgcccgg acactggcgg tttgcgcgca gtcgctggcc tgcgccggac gtctggagga
     8401 agccatacga accgcgcacg cggcgaatga cttcgacccc gtagagccgt atgcgaatgc
     8461 cgtgctcgga gaactcctct gggccacggg cttcccgggc atggcgatct cacacttgcg
     8521 ggagagcatc gaaatcgaac ccggctgcgc tcccctccac tgggcactcg gtctcgcggc
     8581 atcaggaaca caggatcacg tgcttgccct acgtcatctg gaagtggcgt gggagctttc
     8641 ggacggcgcc ggctatgcgg gagccggact tgcgcacgca ctcgctatgg acggccagcc
     8701 atcccgcgca ttcgagttgc tgcacgcgct cgaagaacgc gctgcggaat gctggtttga
     8761 acggagcttt gtgtacctgg cgctgggaga tgttcagaaa gccctcaatt cacttctcaa
     8821 agcgattcaa tcgagatcga tctggtatcc ccgcatctac gtatggcctc aatttgccca
     8881 actgcgatca cacccgcaag ttgcggcggc ggttgatacg tccgaagact ccactctcca
     8941 agcatcctcg gcgggaggca acggcggata gccctgcccg ggcctccgcc gcaccccgat
     9001 catacgaagc tgcgcacccc cgcggcttca tccgagaaaa cttcaaacac cgtgtacaac
     9061 ttcgtgatct ggagcagatc ctgaacacgg tgcgtgagat tcagcagctt caactgtccg
     9121 ccattattgg tgacagtcgt gaatgcagta accaattccc cgataccgga actgtcgata
     9181 tacgacactt cggccaggtt aatcagcaac ttcttattgc cctttgcaat gtgatcgcgg
     9241 acggtatcgc ggaacgtact cgaaccctcg cccagcgtga tcctgccggc cgcgtctatc
     9301 acggtcacat cgcccacttg tcgactactc aacttaacgc tcaatgatgg aacctcctca
     9361 aatgaaccct cacgaactgc gcgtacggta gagcaccagg ctcagttctg ttccgccgct
     9421 ttcgagatgc ctgacatgaa actcgtccac gaatgccctg atgagcagga taccgcggcc
     9481 ggattgacga agcaggtttt ccgccgccag cggatcggga agcgatgcca cgtcaaaccc
     9541 ctcgccttcg tcctgaatac gtatcgtcag ccggtccggc gcttcagaca ccgccaggcg
     9601 taccttctta tgcgcgctgt atcgatttcc gtgaacaacc gcattcacca ttgcttcgcg
     9661 aatggcgacg ccgatcttat gcagttcgtc ctcgccgaag ccgacatccg tggccgcacg
     9721 caggacaatc tgctcggcgg tatccacgct gtccagggtc gaatccagca ccgtttccac
     9781 gctcctgttc tcgccgctgc ggtttgatgg cataatttcg agggcggccg tggcgtggcc
     9841 cggtcgaacc cgttaactta ttacgaaaga aggacaaaag tcaaggccag ggcattctcc
     9901 gagattcacc gggtggcaga aaaactcgta gctgagccgc gacggcaacg gagcggtcat
     9961 gtcgcacgcc cggcactctc cggctcctca gcgcaccttc tccggcttcg cgaaagctcc
    10021 cttcatttgt gtgggcaact gccagacgcc gccgtttctg tccgcaaagt aaaggctgga
    10081 atcggaaggc gccagggcgt tgccgtccgc ccaaattgca taaaagtccg gatgcgcatg
    10141 aagagggcgg cgcacgtagg tatggttgta tctgctgccc tgcgtcaatt ttcttaccat
    10201 tttccagctt ttgccttcgt cgccgctggt ccacatctcg atctcgccgc cggtgccatg
    10261 cggctgaggt ccggcgccgg ttggggcgat gaggcgccag gtcccgtcgc tctcgagata
    10321 gatcgagccg taatcgtagt tgtgatcgct ggctgcgacg ggttgaaaat cccaattctt
    10381 actggtccag cgggcggtcc ggaacatgcg cgggccgttt tccggaccag actcgtatcc
    10441 cttgcttgtc atgtaaagaa taaccggccg gcccctgctg tcatactgca cgtccttcaa
    10501 atacacaagc agtccctggc tccggtaatc gcggacaaga gccgggttct gttctgtagt
    10561 gaccggaatg tcgacggtct cgccgcccgc cgtgacccac gtatcgccga agtcgcgggt
    10621 ttcaaggtaa tagaggttcg tgcgctggtt caggcccaat ggcggaggat gaatgttaaa
    10681 cgcggttcca acgcgcgcgc cgccggacca actcacctgg taatggccct gtagcgcaaa
    10741 ggcaagccgc cgtggcgaac tccattcccg gccatcggga ctgaccatcc agtacaaggc
    10801 acggcccggg gaccggccct tctccgcctt ctgataccgc gtgtgaagga acaggaatcc
    10861 ccggcccggc acataccaag gttgcggata ggagaaattc gtctcctgaa tcagctcgaa
    10921 atcgatgatg gaatacggct tggcgctctt atggatgtaa gacggccggg aggtgccgtg
    10981 cgcgctcgaa aagatccaga tgtaaccctc gccgtcgatg gagatcgtgg ggttgtcgtg
    11041 ggcgtcatcg gttttcttgt ccagcagcaa aacgggccgg gggacctcgc cggtcgcatg
    11101 atcgaagtag gagaccatgt gcagcagccg gttatgcgtc cctttggccg tccctccgta
    11161 gcagaagaag gttttgttcg cctctttcga ataaatcgcg atgggagcgt gttgctgcgg
    11221 ataagtgacg aagccgccgc tatatttgta aacgtacttg tcgtttgaag gttggttgta
    11281 ataccagatg ccgcggtaac cgtcttccgt cggccgctcc gccgaaagtc cgggcatagc
    11341 cgcgagcagc agtaggatgc tgaatctcat cgttcttaga atagaaccta tgagcacgga
    11401 aacgttccgc cggcactgcc tctcttttcc acacgcgacc gagcaggtgc aatggggcga
    11461 caacctggtg ttcaaggtgg gcggcaagct gttcgcaatt acgtcactgg agccgggagg
    11521 ccacttcgcc tcgctgaaat gttctcccga acggtttcac gaactggtgg agcgtcccgg
    11581 aatagtgccc gctccatacc tcgcgcgagc gtattggatc gcgttggaac gcgaggatgc
    11641 ggccgggcac gcagagttgc tcgaactcct gcgcgaggct tatgagatcg tcctggcggg
    11701 actaccgaaa aaaattcgcg agagccttgg aaccacgaac gcaaaccaac ccaagcgcag
    11761 ccgcaaacga acggcgtaat aggacgttac gcctatttcc gccggctttt ctccacccgg
    11821 attaccttga catccgacat cgcgatcagc cctttgccga tcatctgctc cacttccggt
    11881 accaggaggg ccaggttttc ggctgtatcc acgatgctga tcagcaccgg gcgctcttcg
    11941 cggagggggt gccccccacc tggcgggctg tcgccgtagc cttcaatgcc gggaatcacc
    12001 gtcgcaccag cgatccctgt ttcctggcac ttggccacga tggcttcgta cagcggcttg
    12061 ccgtcgaaca cgtcctgttc accgatatgg atccgaagca atttcgaggg ttcaccgatc
    12121 gacattggag atctccggat ggttgtggat aatgcggatg atatccacgt ccgagacaac
    12181 tatgaggccg tcctccagca tctcttcgat ggccgtggcc gcctccgcga tgcggagcgc
    12241 cgtatcgatg gtggatatca tgatcgggcg atctttggaa agccggcgag actgctcgct
    12301 gtcagggcct gtctttgccc catatccgag aatgccgcgg tagacggttg caccggccac
    12361 ctgcaacgtc tgcaacttct tcacgatggc ttcgtatagg ggttcaccct gccattggtc
    12421 tgcttcgccc aggaagatgc gcagcatctt cgcgcggccc tgcctgcgtt cctggggatg
    12481 tctggttact tccgtttcag tcattttcta ttggtcgtgt caatcgacta tttcaagatg
    12541 ccccggcgct ccagaaaggc gaatatcctc gggtatatct ctgccaactg ctcttgcgaa
    12601 aagccgtgct tgccgccggc gatgggaatc agctccgcat cggcgccctc gtcgcgcagc
    12661 tccctggtaa ggttcacacc gtgttcatag ggaacggtct catcggcatc gccgtgtatg
    12721 gtgagaatcg caggcaggtc tttccggacg tagctggcag gtgagacgcg cctggcaagc
    12781 accaggcggg cttcctgctc cggaacccat gtcaccgcat atttgcgcat gttagggcct
    12841 tcgagttgat cgccaacatc cgtgatgcca taaaaattca cgactgccgc aactttcgcc
    12901 ggtttgccca gccgggccga tttcggtacc atgcccacca tcaaggccag gtgcccgccc
    12961 gccgaaccgc cggtgaccac aatacggtcc cggtcgacgt tatagcggcg tgcgttgtcg
    13021 cgaaaccagt tggctgcctt caggacatct tccactgctg ccggggcggg cgcggctttc
    13081 gccagccggt actccacgtt cgcgacgacg aatccttttt caaggtacgg cagacagaag
    13141 tttcgaacca tgctttcctt tgtgccgttc acccatccgc ct
//
LOCUS       DASTMK010000163         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_296569, whole genome shotgun sequence.
ACCESSION   DASTMK010000163 DASTMK010000000
VERSION     DASTMK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296569"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFQ79_07145"
     CDS             <1..675
                     /locus_tag="VFQ79_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0139466.1"
                     /translation="EKERKWQAGDKEFTTPHGRSIFVQDALADGPILAFEMNGEPLAA
                     EHGYPLRLILPGWYGMANVKWLRAIEVIDRRYEGRHMARNYHSLRKAGTAGSGIWLDT
                     SISRMNLKSVVARVIRKRSGTQVEHVIHGAAWSGESPVRTVEIRVDSGPWVEARVDRR
                     ESDAAWLFWSCLWKDAKPGPHTLVSRAIDTRGAIQPTEEELRERLASAREDNAQWPRT
                     LIVPPY"
     gene            642..2240
                     /locus_tag="VFQ79_07150"
     CDS             642..2240
                     /locus_tag="VFQ79_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0139467.1"
                     /translation="MASYLDRAPILTRRAVLSLVAPAAAFQRNETKLGKIDFPASGSP
                     QARERFLRGVMYLHSFEYDDAREEFAAARQADPAFVMAYWGEAMTYNEPLWYRQDAES
                     ARKALARLAPARDERLKRAATERERAWLGAVEVLYGSGSKEQRDFAYSDAMRQLWSAY
                     PDDLEAASFYALSLLGTSHHGRDTRIYMQAAAAAEEVFARNREHPGALHYLIHCYDDP
                     LHAPLGLRAARIYAKVAPAAAHALHTPSHIFIAMGMWDDVVSSNEDSWAASEARYKRK
                     NLPLEDRGYHALWWLGYAYLQQGRYKEARGLLSIVEEDAVRSPSPFIRFHAAHMRSAY
                     AVETGQQEVYQKALDLSDLDIPAAVSYLFAHGFLAAVQGDAEPAAAALTRIRERRDAG
                     RTHEPAGGHEHHFYPPDLQVAGIVEKQLEGVIAMAGRETDRGLQLLSDAAAIEDKLPF
                     EFGPPVPPKPAHELLGEMLLAANRPREAQKHFALALERAPRRSLSLLGIARAARKAGD
                     AVSAKAAYAELAQVRKKADVEWAE"
     gene            complement(2345..>3554)
                     /locus_tag="VFQ79_07155"
     CDS             complement(2345..>3554)
                     /locus_tag="VFQ79_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139468.1"
                     /translation="TDKTVLRTGYGISIDPYSLSRPLRTNHPILIELVEPSPNNMLPA
                     SRLADGIPPIQPVDLDNGIIDVPANVSTQTVPLNFRRGYIQSWNFTVQQDLKWGFVGE
                     VGYVATRQIRQLGYQQLNWAPVGGGNNGRQLFQKFGRTANTREVSPIGGSHYDSLQAQ
                     LQRRFAQGISMNVAYTFAKSITNSGSDVSDGTPPVAIPDLYYLNRSVSAFDRTHNLQI
                     TNITDLPFGRGRRWLKDNGVLTTIAGGWQLNSIVSLMSGRPFSVTASGTSLNAPFSTQ
                     RADQIKPEVEILGGVGRGTPYFDPFAYAAVNDARFGTAGFNSLRGPGRVNWDLSMFRD
                     FRITEKVNLQFRAEGFNITNTPKFGNPGANVSNIQYNADGSVRNLNGFGEITSASEER
                     EFSLGLRLSF"
BASE COUNT          680 a         1073 c         1118 g          683 t
ORIGIN      
        1 gagaaagaac gcaagtggca ggccggcgat aaggagttca ccacaccgca cggtcgcagt
       61 atcttcgttc aggacgctct tgcggacggc ccgattctgg ctttcgagat gaacggcgaa
      121 ccactcgctg ccgaacacgg ctatcccttg cgcctgattc ttcctggatg gtacggcatg
      181 gccaatgtga agtggctgcg cgccatcgag gtgattgacc gccgctatga aggccgccac
      241 atggcccgca attatcacag cctgcgaaaa gccggcacgg cgggtagcgg catctggctc
      301 gacacgtcca tttccagaat gaacttgaaa tccgttgtgg cgcgggttat acgcaaacgc
      361 tccgggacac aggtggaaca cgtcatccat ggggcggcat ggagcggaga gtctcctgtc
      421 cgtaccgttg aaatacgtgt ggattccggc ccatgggtgg aagcgcgcgt ggaccgccgt
      481 gaatcggatg ctgcgtggct gttctggtcg tgtctgtgga aagacgcaaa gcccgggccg
      541 catacactgg tatcgagagc aatcgacacg cgcggcgcca tccagcctac agaggaagaa
      601 ctgcgggagc ggctggcgag cgcccgcgaa gataatgcgc aatggcctcg taccttgatc
      661 gtgcccccat attgactcgg cgtgccgttc tcagcctggt tgcgcccgcc gctgccttcc
      721 agcgcaatga aacaaaattg gggaagattg attttcccgc gtccggttct ccccaggccc
      781 gggagcgatt cctccgcggc gtaatgtatt tgcacagctt cgaatacgac gacgcgcgcg
      841 aggagttcgc cgccgcccgg caggccgatc ccgctttcgt catggcgtac tggggcgaag
      901 ccatgacgta caacgagccg ctgtggtacc ggcaggacgc ggagagtgcc cgcaaagcgc
      961 tcgctcgcct ggcgcctgca cgcgatgagc gcctgaaacg cgcggcgacc gaacgcgaac
     1021 gggcctggct tggcgccgtc gaggtgctct atggctccgg atcgaaagag cagcgcgatt
     1081 ttgcgtactc cgacgccatg cggcaactct ggagcgcgta tcccgacgac ctggaggccg
     1141 cgtctttcta cgccttgtcg ctgctcggca caagccacca cggaagggac acgcgcatct
     1201 acatgcaagc cgccgctgcc gccgaagagg tgtttgcgcg gaaccgggag cacccgggcg
     1261 cgctccacta cctgattcat tgctacgacg atcctctcca cgcgccgctc gggctccggg
     1321 ccgctcgcat ctatgccaag gtggctcctg ctgccgctca cgctctgcac acgccctcgc
     1381 acatcttcat cgcgatgggc atgtgggatg atgtggtttc ctcgaacgag gattcctggg
     1441 ccgcctcgga ggcccgttac aagcgaaaga acctgccgct tgaagaccgc ggctatcacg
     1501 cgctctggtg gcttgggtac gcttacctgc agcagggacg gtataaagaa gcacgcgggc
     1561 tgttgagcat cgtagaggaa gatgctgtgc gcagtccctc tccgttcatc cggttccacg
     1621 ccgcgcacat gcgctccgcc tatgccgtgg aaacaggcca gcaggaggta tatcaaaaag
     1681 ccttggatct atcggacctc gacatcccgg cagccgtcag ctacctgttc gcgcacgggt
     1741 ttctcgcggc cgtacagggc gatgccgaac cggccgctgc tgctctcact cggatacggg
     1801 agcgccgcga cgcgggcagg acgcacgagc ccgccggcgg gcatgagcac cacttctatc
     1861 caccggacct ccaggtcgcc gggatcgtag agaagcagct cgaaggagtc atcgccatgg
     1921 caggccgcga gacagatcgc ggcttgcaac tgctttcgga tgccgccgcg atcgaagata
     1981 aactgccttt tgaattcgga ccgcccgtgc ctccgaaacc ggcgcacgag ctgctgggag
     2041 agatgttgct ggcggccaac cgtccgcgtg aagcgcaaaa acatttcgcg cttgctttgg
     2101 agcgcgcgcc ccggcgctcc ctgtcgctgc tggggatcgc ccgggccgcg cggaaagcag
     2161 gtgatgcggt cagtgcaaaa gcggcctacg cggagcttgc ccaggtgaga aaaaaggcgg
     2221 acgttgaatg ggcggagtga ggttcgctca ctccgcctgc tcgccaagac cgcttgctgg
     2281 cgcgcgcggc tccgtaatcg cttctgagcc gcgaacgtga gtgagcggtt gtctgtgaca
     2341 gtccctaaaa gctgagccgc aagccaaggc tgaactcgcg ctcttccgac gcgctggtga
     2401 tttcaccgaa cccgttcagg ttgcgaacgg agccgtcagc gttgtactgg atattgctca
     2461 cattggctcc cggattgcca aacttcggtg tgttggtgat gttgaaacct tccgcccgga
     2521 attgcaggtt cactttctcc gtgatgcgga aatcgcggaa catgcttaaa tcccagttca
     2581 cgcggccggg gccgcgcagc gaattaaagc cggccgtgcc gaaacgggcg tcgttcaccg
     2641 ccgcataagc aaagggatcg aaatacggcg tgccgcggcc gacgcctccc agaatttcca
     2701 cttccggctt gatctggtcg gcacgttgcg tactgaacgg tgcgttgagc gaggttcccg
     2761 atgcggttac cgagaacggc cgtccggaca tcaggctgac gatgctgttg agttgccagc
     2821 cgccggcgat tgtggtgagt acgccgttat ccttgagcca gcggcgcccg cggccgaaag
     2881 gcaggtccgt gatgtttgtg atctgaagat tgtgcgtacg gtcgaaggca ctgacggagc
     2941 ggttcagata atacaaatcg ggaatggcga caggcggcgt gccgtcgctt acgtccgaac
     3001 cggagttggt gatcgacttg gcaaacgtat atgccacgtt catcgatata ccctgcgcga
     3061 agcggcgttg gagctgcgct tgcagcgaat cgtagtggct tccgccgatc ggcgatacct
     3121 cgcgcgtatt cgccgtccgt ccgaacttct ggaacagttg ccgtccgttg tttcctccgc
     3181 ccaccggcgc ccaattcaac tgctggtagc cgagttgccg gatttgccgg gtcgccacat
     3241 atcccacttc tcctacgaag ccccacttca aatcctgctg cacggtaaag ttccacgatt
     3301 ggatgtaacc gcgccggaaa ttgagcggaa cggtttgcgt ggaaacattc gcgggcacat
     3361 cgatgatccc gttgtcgagg tctacgggct gaatcggcgg aattccatcg gccagccggc
     3421 tggccggaag catattgttg ggagacggtt ctaccagttc gatcaaaatc gggtggttgg
     3481 tacgcagtgg ccgcgagagc gagtacgggt cgatgctgat accgtagccg gtgcggagga
     3541 cggtcttatc agtc
//
LOCUS       DASTMK010000164         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_264195, whole genome shotgun sequence.
ACCESSION   DASTMK010000164 DASTMK010000000
VERSION     DASTMK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264195"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VFQ79_07160"
     CDS             complement(<1..978)
                     /locus_tag="VFQ79_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139469.1"
                     /translation="MLLRNLCSLALGLSLAFAQDVPVAFTGGRVIPIAGPEIPDGVVV
                     IEKGKIVAVGPSASTRIPPNARRQDIRGKTVMPGLIDSHSHIGGIDGGDSSVPIQPEV
                     RVLDSLDVRDARIQKAQAGGITVVNVMPGSGHLLSGQTLYLKLRDGRTVYDLLIPGAD
                     GKPLGGIKMANGTNSRRNAPFPGTRAKSAALVREEYVKAQEYREKIRKTNGDASKLPV
                     RDLRMEALAEVLDGKRVVHHHTHRHDDILTVLRVAKEFNIRVVLHHVSDGWKVAEELG
                     AAKAMCSIIVLDSPGGKEEAKDVSLVTGGALEKAGALVGFHTDDGITDSR"
     gene            1114..1788
                     /locus_tag="VFQ79_07165"
     CDS             1114..1788
                     /locus_tag="VFQ79_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU0139470.1"
                     /translation="MKITISKENYLKAIAEAESEGETVIAATLARWLNVSAPAVAMAV
                     RRLKRDGLIQVAAQGEITLTRAGRDIATRLLNRHHLIERMLTEIFGMEWYKVHDEAEQ
                     LEHAVSEDFERRLAERLGTSQACPHGNLVGLDRPEDRRQKGLIPLAEAPGGTDATVVS
                     VFERDRQLLEYLDTLGVRPGARFGVLNQNYDQTLTLKINGRDVPLGRAAAEKVWVRMA
                     DPSARM"
     gene            1797..>2923
                     /locus_tag="VFQ79_07170"
     CDS             1797..>2923
                     /locus_tag="VFQ79_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139471.1"
                     /translation="MPSKLFVFMLPLTLLAQGEWTIYSSRGIEVISNASNRAARRTLN
                     EIEQFRNAVGTMLGVQDAGSRWPIRVLVLKRSRDLTVPPSLRLARDAYIGGVIAESDL
                     PALFRRDLTRILMDDNTGRMPANIEKGLETLFTTLQIDGTRVTLGAPPPAPERDRDWA
                     RVHMLAVTPEYSGKLRVLLGNLQRGVPEDVAYRNAFGKLPQQIEREVDTYARGENSGT
                     IPLSGLPLNPQRDFVEKPYSDLAAKIALADLTPGRAAYEAILKLEPGAAAAHEGLGLI
                     ALREKQPEEARRHFAAAVEAGSRSAQAQLEAGNARQAAELNPKWAEPHIRLARDTPEP
                     AQKIDHLTKATAIDPRNSKLWKELAELLEAENRFADAAKAWA"
BASE COUNT          588 a          892 c          913 g          530 t
ORIGIN      
        1 gcgcgagtcg gtgatgccat cgtcggtgtg aaacccaacc agcgcgcccg ctttttcgag
       61 tgcgcctccg gttacgaggc tcacatcttt ggcttcttcc tttccgccgg gactatcgag
      121 gacgatgatg gagcacattg ccttcgccgc gccgagttcc tccgctactt tccagccatc
      181 gctgacatga tgcaggacga cgcggatatt gaactctttc gctacgcgga gcaccgtcag
      241 gatgtcgtcg tgccgatgcg tgtgatggtg gacgacccgc ttcccgtcca gcacctccgc
      301 caaagcttcc atacgcaaat cccgcaccgg cagcttgctc gcatcaccgt tcgtcttgcg
      361 gattttctcg cgatactcct gcgctttgac atactcttcg cggaccagtg ctgcggattt
      421 cgctcgtgtg ccggggaacg gggcgttgcg ccgggaattg gtgccgttcg ccatcttgat
      481 tccgcccagt ggcttgccgt ccgcgccggg aatgagcagg tcgtacactg tgcggccgtc
      541 ccgcaacttc agatacagcg tctggccgct gagcagatgg ccggaacccg gcatcacgtt
      601 gacgacggtg atgcccccgg cctgcgcctt ctgaatccgg gcgtcccgca cgtcgaggga
      661 gtccagaacg cgcacctccg gctgaatggg aacgctcgaa tcgcctccgt cgatgccgcc
      721 gatatggctg tgcgagtcga tcaggccggg catgaccgtc ttgccccgga tatcctggcg
      781 gcgcgcattg ggcggaattc gtgtggaagc ggacgggcct accgccacga tttttccctt
      841 ctcgataacg accacaccgt cgggaatttc cgggccggcg atgggaatga cacggccgcc
      901 ggtgaaggca acggggacat cctgcgcgaa agcaagagac aacccgagcg cgagcgagca
      961 aagattccgg aggagcataa aagcagaccc gcaaaagaag ccgattccac cgaatcagat
     1021 acttacttgt gggtttcctc gaaccaattt atcatcctcg aacgcgcgga tgaggaaatc
     1081 cggcacgcgt atcacgctac aattctgagt aaagtgaaga tcacgatctc caaagagaac
     1141 tatctcaagg cgatcgccga agctgaatcc gaaggagaga cggtaattgc agcgacgctg
     1201 gcgcgctggc tgaatgtatc tgcgcccgcc gttgcaatgg ccgtccggcg cctgaaacgg
     1261 gatggactca tccaggtggc ggcccagggc gagattacgc tgacgcgggc cgggagggat
     1321 attgcgaccc gcctgctgaa ccgccatcac ctgatcgaac gcatgctcac cgagatcttc
     1381 ggcatggagt ggtacaaggt tcatgacgaa gccgagcaac tggagcatgc ggtttccgag
     1441 gatttcgaac gccgcctggc ggagcgcctg gggaccagcc aggcctgtcc ccacggcaac
     1501 ctcgtgggat tggaccgccc tgaggaccgc cggcagaaag ggctgattcc cctggccgaa
     1561 gcgcctgggg gcaccgatgc taccgtcgtc tccgtcttcg aacgtgaccg ccagttgctc
     1621 gaatacctcg atacccttgg ggtccgtccg ggggcgcgct tcggtgtcct gaatcagaac
     1681 tacgatcaga cgctcacgtt gaagatcaac ggccgcgatg tgccgctggg ccgcgccgcg
     1741 gcggagaagg tctgggtcag gatggcggac ccgtctgcga gaatgtagat acggtgttgc
     1801 ccagcaagct tttcgtcttt atgctgccgc tcacgctgtt ggcacaaggc gagtggacca
     1861 tctatagctc tcgcggcatt gaggtcatct ccaatgcaag caaccgcgcg gcacgccgga
     1921 cattgaacga aatcgagcaa ttccgcaacg cggtgggcac catgctcggc gtccaggatg
     1981 ccggttcgag gtggccgatt cgcgtcctgg tgctgaaacg cagccgggac cttacggttc
     2041 cgccatcgct ccgcctggcg cgcgatgcct atatcggagg cgttatcgcg gaatcggatc
     2101 tgccggcatt attccggcgg gatcttacgc gcattctgat ggacgataac acgggccgca
     2161 tgcccgccaa tatcgagaag ggactggaga cgcttttcac caccttacag attgatggca
     2221 cgcgcgtaac attgggcgct cctccgcccg cgccggagcg tgaccgggat tgggcgcgcg
     2281 tgcacatgct ggcggtgacg ccggagtata gcggcaagct gcgagtgctg ctcgggaacc
     2341 tgcagcgcgg cgttcccgag gacgtagctt accgaaacgc gttcggcaag ctgccacagc
     2401 agatcgaacg cgaagtggat acctatgcgc gaggggagaa ttccggtacg atcccacttt
     2461 cgggtctccc gctcaatcct cagcgcgatt tcgtggagaa gccctacagc gatctcgcgg
     2521 cgaaaatcgc tctggcggat cttacgccag gcagggcggc gtacgaagcc attctgaaat
     2581 tggagccggg cgccgcggcc gcgcacgaag ggctagggct catagcgctg cgggagaagc
     2641 agccggagga agcgcggcgg catttcgcag ccgctgtgga agcgggaagc agaagcgccc
     2701 aggcacaact ggaggccggc aacgcccggc aggccgcgga attgaatccg aagtgggccg
     2761 aacctcacat ccgcctggcc cgcgacacac cggaaccagc ccaaaaaatc gaccatctga
     2821 caaaagccac ggcgattgat ccgcgcaatt cgaaactgtg gaaggaactt gccgaattgc
     2881 tcgaagccga aaaccgtttt gcggacgcgg caaaagcctg ggc
//
LOCUS       DASTMK010000165         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_360338, whole genome shotgun sequence.
ACCESSION   DASTMK010000165 DASTMK010000000
VERSION     DASTMK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360338"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VFQ79_07175"
     CDS             complement(<1..1203)
                     /locus_tag="VFQ79_07175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0139472.1"
                     /translation="MRLAFFSPLPPSRSGIADYSAALLERLRHSMQVDVFDSEPRDFS
                     PEGYDAILYQIGNNPDHTFVYDAALKYPGIVVMHEANLHHLVAEMTIRLGDWDSYLSE
                     LELNGGAQAVEYGRRVQALEAGPDYDGVPMLRRLLSSARALVAHSHTVEASMRAAGFT
                     GPAAVIPHGAWLLETDRMGYRGRLGIDNSTPLIGVFGHLKPYKRIPESLRAFRRLLRV
                     QPDARMILVGQPHPDLPLQPLITSLGLAGAVRVLGRVQMEDFSGYLGACDIVLNLRYP
                     TVGETSGTLLRALGLGKAVLVSGVGAFCEFPAEVCLHVPVDATEEDVIFEYLNLLVSR
                     PDLAAHLGTKARTWVERECSWDLVASRYASFLEAVAKGTSWQDERAPEAHEDGEPSAA
                     SAPVEPEYL"
     gene            complement(1204..1893)
                     /locus_tag="VFQ79_07180"
     CDS             complement(1204..1893)
                     /locus_tag="VFQ79_07180"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU0139473.1"
                     /translation="MIELALLSLWLLATPEQKPAESGNEVLEYNINWPSGLSLGEAKL
                     TASSVSEEGKKLLSFEFNIDAAVPGFAVRDEIRSTATSEFCSASLEKHLSHGNRKPRE
                     RTDFNQQARVATRATEGGGKSELPLPACAKDALTFLHYLRSELKQGRLPPPQTVFFGA
                     PYQLRLEYGGTRSMQLGDSMVQADRLTGTVKGAVSENRFEIVVARDASRTPLLVSLPL
                     ALGTFSMELVQ"
     gene            complement(1886..>2479)
                     /locus_tag="VFQ79_07185"
     CDS             complement(1886..>2479)
                     /locus_tag="VFQ79_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0139474.1"
                     /translation="YILTVGDLQPRKNQTGLIRAYEQLVRNHPEIRHSLVLVGKQTWH
                     APQVLEAARRSDLANRIVFTGWVSDEELLQIYGGCDLFVFPSFYEGFGLPILEAMACG
                     RAVACSGTSAMPEVADSAAILFDPRSVEEMVRAMRDVLLDAGLRGRLERLGLQRASLF
                     SWDRAARRTLDVYYDVAGRSMRERSERARSVPVTIHD"
BASE COUNT          529 a          780 c          711 g          459 t
ORIGIN      
        1 cagatactcc ggctccacgg gcgcggaagc ggcagacggt tcgccgtcct cgtgtgcctc
       61 gggcgctctt tcgtcctgcc aggatgttcc cttggcgacc gcctccagaa acgaagcgta
      121 acgcgaggcc accagatccc aactgcactc gcgctccacc catgtcctcg ccttcgtgcc
      181 aaggtgagcg gcgagatcgg gccgcgacac cagaaggttg aggtactcga atatcacatc
      241 ctcctcggtg gcgtccacgg gaacgtgcag acaaacctcg gctggaaatt cgcaaaacgc
      301 gccgacaccg gagaccaata ccgctttccc cagcccaagg gcgcgcagca acgtgccgga
      361 cgtttctcct accgtcgggt atcggaggtt caatacgatg tcgcacgcac ccaggtaacc
      421 cgagaaatcc tccatctgca cacggcccag cacgcgaacg gcaccggcaa gccccaggct
      481 ggtaatcaac ggctggagcg gcagatccgg atgcggctgc cccaccagta tcattctagc
      541 gtcgggttga acgcgcagca gcctgcggaa cgcacgtaga gattccggaa tgcgtttata
      601 cggcttgaga tgcccgaaaa cgccgatcag cggcgtggag ttatcgatac ccaaccggcc
      661 gcgataaccc atccggtctg tctcgagcaa ccatgctccg tgaggtatga ccgcggccgg
      721 tcctgtgaag ccggctgcgc gcatggatgc ttccaccgtg tggctgtgag cgaccagcgc
      781 cctggcagaa ctgaggagcc gccgcaacat gggtacgccg tcgtaatcgg ggcccgcttc
      841 gagtgcctga acacggcggc catattccac cgcctgcgcg cctccattga gttcgagctc
      901 actgagataa ctatcccaat ccccgagccg gatcgtcatc tcggctacaa gatgatgcag
      961 gttcgcttca tgcatcacca cgattcccgg atactttaaa gcggcatcgt agacaaacgt
     1021 atgatccggg ttgtttccga tctggtaaag aatcgcgtcg tagccttcag gggaaaagtc
     1081 gcgtggctca gaatcgaaaa cgtccacctg catggagtgc cggaggcgtt cgagaagagc
     1141 cgctgagtaa tcggcgatcc cgctcctcga cggtgggagc ggcgagaaaa acgccaggcg
     1201 catctactga accagttcca tcgaaaacgt gcctagcgcc aacggaagac tgacaagcaa
     1261 cggcgtacgc gatgcatcgc gggcaactac gatctcgaag cggttctccg agacggcgcc
     1321 tttcacagtt cccgtgagcc tgtcggcctg caccatggaa tcgcccagtt gcatgctacg
     1381 tgtgcctccg tactccagcc gcaactggta gggtgctccg aaaaatacgg tttgaggggg
     1441 cggcagccgg ccttgcttca actccgaccg caaatagtgg agaaaggtaa gggcgtcttt
     1501 tgcacacgcg ggaagcggta gttccgattt cccgccgcct tcggtagctc gtgtcgcaac
     1561 tctcgcctgc tggttgaaat cggtccgctc ccgcggcttt ctgtttccgt gcgacaggtg
     1621 tttttccagg ctagcggaac agaattcaga cgtagccgtg gagcgaatct catcgcgcac
     1681 cgcaaacccc gggacagcgg cgtcgatgtt gaactcaaac gagagcagtt tctttccctc
     1741 ttcggagaca gaagacgccg ttaactttgc ctcacccaga ctgagtccgc tgggccagtt
     1801 gatgttgtat tcgagcacct cattccccga ttccgcgggt ttttgttcgg gggtggcgag
     1861 gagccataga gataggagtg caagctcaat catgaatcgt cacgggaacg gacctggcgc
     1921 gttcagagcg ttcgcgcatc gaccgtcctg ccacatcgta ataaacatcg agcgtccggc
     1981 gcgccgcccg gtcccaactg aatagagagg cacgctgcag gccgagccgc tcgaggcggc
     2041 ctcgcagccc tgcatcgagc agcacgtcac gcatggctcg aaccatttcc tccaccgagc
     2101 gcggatcaaa aagaatggcc gctgaatccg cgacctccgg catggccgac gtgccggaac
     2161 aagccaccgc gcgcccgcag gccatcgctt ccagaatcgg cagtccaaaa ccttcgtaga
     2221 aagacggaaa cacgaacagg tcgcacccgc cgtaaatctg cagcaattct tcgtcgctca
     2281 cccatccggt aaaaacaatg cggttggcca ggtcagagcg ccgggctgcc tccagaacct
     2341 gtggcgcatg ccacgtctgc tttcctacca gcaccagcga atgccgaatt tccggatggt
     2401 tgcgaacaag ctgctcataa gcccggatga gtccggtctg gttcttgcgc ggctgcaggt
     2461 cgcctactgt caggatgta
//
LOCUS       DASTMK010000166         8758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_296693, whole genome shotgun sequence.
ACCESSION   DASTMK010000166 DASTMK010000000
VERSION     DASTMK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296693"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFQ79_07190"
     CDS             complement(<1..339)
                     /locus_tag="VFQ79_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139475.1"
                     /translation="MTSIPHFGRIRRLALSGVTCLSAEERRSLARFLEAGGTVIATGP
                     TGHHDERARPLAKGWLARFGIPAALEEPVRGGAFPPNSTLGKTPETARCRVPDEFRTK
                     MSGGWFRTTVG"
     gene            322..2586
                     /locus_tag="VFQ79_07195"
     CDS             322..2586
                     /locus_tag="VFQ79_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU0139476.1"
                     /translation="MRNRGHRPVPMYDWKIDAWAQPWKVRCPHCNGLFPKNDFEAFYR
                     SGLDERGVFDPKRADRALLFNAEHPNPSDPLHRFGVDDGEGYVEGDKRWRFIGAYLLY
                     GHWSQRIEAGLRHLATAYTVTGDRAYAHKAGVLLDRIADLWPSFDYATQGLVYERARY
                     GGGVAGYVYYAITSAYTVMRLVSTYDKIFDGIRDDTALVEFLRGKDPRKNSFAEIQQN
                     IENNILRHVLANPRQIRTNYPGAERTLALTRTVLEWPANRSQLRSDLDEIVRKGVAAD
                     GLSGEKGLSSYAAIAPRELAEILAEYSHLDPGLLPYLLERNPKFRDTYRFHFDTWINR
                     EYYPNSGDAGSFARKSLEYPALTFNGPVYSLLGRLARVTRDPLYMQLAWMGNGGRAEG
                     LPHDIFAEDPAGFAAEVKAAVSRHGPWPAAASVNKKDWRIAILRHRTDPASAVWLDYD
                     SVPEGDLKSHYHFDAMNLGLYFGGLNLLPEFGYPAVQFGDWHTPQALWHRKTAAHNTV
                     VVDGRDQAGGPAECTLWNAGGPLQAIRASSPSQIKGSVYERTIAMIETGPTGYYVFDV
                     FRVQGGKEHAKHTHTTFAAPTVFGVRLTPSPPLYDSGTLMRAFQTGRAGPSWGVDWKI
                     EDKHGYVQPPRDLHLRYTDLTRDAEAYTAESWTVASATSTTEYWIPTAIVRRRSDSGD
                     LTSTFVGLLDPYEGQPQLKRITRLDRDDAAVQVEVELSGGTRDRLLSNAAGVTLERRD
                     ASGKVILTASAIGR"
     gene            complement(2561..3742)
                     /locus_tag="VFQ79_07200"
     CDS             complement(2561..3742)
                     /locus_tag="VFQ79_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0139477.1"
                     /translation="MERRNFLSLPMVAMQARSPYVSWSWERWREITGENRPVLRTEQT
                     GQAALADLLQFQGGKAVARGDWPERRVSIEQTVLAILGAPPENKPPLAPRILEEVKLP
                     THIRRKLVYQAEPGEFVPAYLLVPHKLESRKPAVVCPHQTVQFGKDEPAGLAGNPRLH
                     MALELVNRGYVTFTYDAACFGERHDPSTGHYGEAIPFYRKHPRWSMMGKMAWDLSRAV
                     DYLQTLDFVDSARIASIGHSHGGYTTLFAMALDPRITVGVSSCGFDTFRHDGNTWRWS
                     HATALMPRLGFYLSSPYLKNGFYAGVPDSETVQTPLDLHYVLAMIAPRPLFLSTSDED
                     SIFPNAGWSARQALARLDPVYQLLGAGDRLDSYFFRGGHGFPPEASERAYAFIDRWLR
                     P"
     gene            3824..4150
                     /locus_tag="VFQ79_07205"
     CDS             3824..4150
                     /locus_tag="VFQ79_07205"
                     /inference="COORDINATES: protein motif:HMM:NF002586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEU0139478.1"
                     /translation="MTSVFWYLLAAAGEIAGCFTFWAWLRLNKSPLWLLPGLVALIVF
                     ATALTRIETANAGRAYAAYGGVYILSSLLWLWAVESVRPDRWDLLGASICLAGAAVIL
                     FGPRVV"
     gene            complement(4216..7674)
                     /locus_tag="VFQ79_07210"
     CDS             complement(4216..7674)
                     /locus_tag="VFQ79_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139479.1"
                     /translation="MRFAVLSVSLCVAAAGVLAQTTSTEILGTVMDPAGASVPSARVT
                     LLRTATGQARETLTNDDGNYVFPLIEVGDYTVKVEREGFKTQEQRGITVQLQQKARID
                     FRMEIGATTETIEVVASGVALKTEDAAVGGNIDNKRVVELPLNGRNVATLAVLVPGVQ
                     YGIRMGLDGTGGFPIPGNGVAISANGQREVNQQVTLDGVIATEPRVNTAAFSPSIDAL
                     EEFKVQTSSYSAEYGQNNGAILQMVVKAGTNNLHGTFWEFLRNDKLAAKDYFLNFQLP
                     AGAREGSKSVLRRNQFGTFVSGPVILPKIYNGKDRTFWSFNYEGRRETRETVEEAAWF
                     PEAFRRGDFSALLTPPIGPNGAPIRSPTIIFDPATGEPFRDSSGRITNIIPASRINKA
                     TQDFINTYQPLPMFQPADVLDINARGSVPNIINTNQYMFRIDHNFSSSDKVFVRYITD
                     RGKWTNGALNPNFTYFVQTLNWNLATQYVKILSPTTLNEFRYGINKADDDTFNPRTNT
                     GFDLDSLGLGSFRVTSGRKLTDREVGIPSTIIAGDRDGGNGWDRNTVHQFADNLSFTR
                     GPHNFKAGFEYRRLILDRAAANVPRGSIACCPGGNSLAGWLMGYFSNTETGEGFPFTA
                     PRQNRWSAYFLDDWKATRKLTINLGMRWDFFQTPRDSAGAWRSLRLDILSRAADGRQL
                     PTVIPEVGLKNWTPYNRENRYYMPRVGIAYRATDKWVMRAGAGWFANAQQLNNYTILN
                     LVPPVSGTIAYQIQQDILTPFSYEYAGRTYQIQTNRLRPGFANQVGNIYGAGSGSVSS
                     VRNTVLVPPDNRSSNHWQWSLDLQRNLPWNTVLTVGYIGSKTNHLDNTITANNPDPAY
                     LDTNIQARRPYPFHISEGEVDANGNPAVFGTGNIRYLDSYGNGNYNALQVSVDKRYDS
                     AGLVLGLAYTYGKALGEGYGRNEGGAGVSNGYQNPRDRRQARARYGFDVTQNAVVNFV
                     YEMPFLRNIRGVPGAFIGGWQMNGVVTLRTGFPFTVTGGSDLNTGGFTLPDRVEDGRL
                     GDAATRQMWYDITAFRRTACNINGRPDLCHYGNAAMDAMVSPGGQNVDFSVYKNWRLP
                     LGEQGRLQFRAEFFNLFNHPNFGQPGGISYTSLNSVIPDGPRNGEIRSLRNPMRIIQF
                     GAKIYF"
     gene            complement(7869..>8758)
                     /locus_tag="VFQ79_07215"
     CDS             complement(7869..>8758)
                     /locus_tag="VFQ79_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0139480.1"
                     /translation="DYAAVQKNATFLKGRKALDFNLTRLDLPVRAGYAAFEMALYDIV
                     GKAFGWPVYKLLGGLAQRKVLVGYWTGRRTPRGMIRVAERAVAGKFTSVKTKCAQGDP
                     VVETAEILAKHAPGIKYIVDPNTRYKSYADFLHVAKALDAIGNALVFEDPFDKNDFEG
                     YRKLRREVKTHVALHLGDPKAMIRAIKEDACSIFNTGGNPGMSGFVANCYLAGAAGIP
                     VWHGSGNDCGIVDASYLHSCAASPNCTMPSDILSFLREDDLIVEPIDIRDSYATVSDR
                     PGLGVDLDKDAIERYRVKA"
BASE COUNT         1654 a         2748 c         2573 g         1783 t
ORIGIN      
        1 gcccaccgtt gtacggaacc atccgccgct catcttcgtc cgaaactcgt ccggcacgcg
       61 gcaccgcgcg gtctccggcg tcttccccaa cgtactattg ggcggaaacg cgccgcctcg
      121 aaccggttcc tccaaagccg cgggaatccc gaaccgggcg agccagcctt ttgccagcgg
      181 acgcgcacgc tcgtcgtgat gacccgtcgg acccgtcgcg atcaccgtcc ccccggcttc
      241 gaggaagcgc gcgagcgaac gccgttcctc cgccgacagg cacgtcacgc cgctcagcgc
      301 caggcgccgg attctgccga aatgcggaat cgaggtcacc gccccgtgcc catgtatgac
      361 tggaagatcg atgcctgggc gcagccctgg aaagtccgct gcccgcactg caacgggctg
      421 tttccgaaaa acgacttcga agcgttctac cgcagcggcc tggacgagcg cggcgtcttc
      481 gatcccaaac gcgcggaccg cgccctcctc ttcaacgcag agcatcctaa tccatcggac
      541 ccgcttcatc gcttcggtgt cgacgatggt gaaggctacg tggaaggcga caagcgctgg
      601 cgcttcatcg gtgcctacct tttgtatggg cattggtccc agcgcatcga ggctggtttg
      661 cgccatcttg ccaccgccta taccgtgacg ggcgaccgtg cctatgcgca caaagccggc
      721 gttctcctgg accgcatcgc cgacctctgg ccttctttcg actacgccac gcaaggcctg
      781 gtatacgaac gcgcccgcta tggtggcggc gtcgccggct atgtctacta cgccatcacc
      841 tccgcctaca ccgtgatgcg cctggtttcc acctacgaca agatcttcga cggcattcgc
      901 gacgacacgg ccctggtcga gttcctgcgc ggtaaagatc cgcgcaaaaa cagcttcgcg
      961 gagattcagc agaacatcga gaacaacatt cttcgccatg ttctcgcgaa cccccgccag
     1021 atccgtacca attatcccgg cgcggaacgc acgctcgctc tcacgcgcac cgtactcgaa
     1081 tggcctgcca atcgctcgca gctccgcagc gatctggatg agatcgtgcg caagggcgta
     1141 gccgccgatg gactctccgg cgaaaaaggc ctgtcctctt acgctgccat cgctccgcgc
     1201 gagctcgccg aaattcttgc ggagtactcg catctcgacc ccggcctgct tccttacctg
     1261 ctggaacgca atccaaaatt ccgggatacc taccgcttcc atttcgatac gtggatcaac
     1321 cgcgagtact atcccaactc cggtgatgcg ggttcgttcg cgcggaagtc cctcgaatat
     1381 ccggcactca cgttcaatgg cccggtgtac agtctgctcg gccgcctcgc acgagtcacg
     1441 cgcgatccgc tctacatgca gttggcgtgg atgggcaacg ggggacgcgc cgaaggcctg
     1501 ccgcacgaca tcttcgccga agatccggct ggttttgccg cagaggtgaa agcggccgtc
     1561 agccgccatg gcccgtggcc ggcggccgcc agcgtcaaca aaaaggactg gcgcattgcc
     1621 atccttcgcc accgcaccga tcccgcctcc gccgtatggc tcgactacga ttcggttccc
     1681 gaaggggatc tcaagagcca ctatcacttc gatgccatga acctggggct ctacttcggc
     1741 ggcctgaatc tgcttccgga gttcggctat cccgcggtgc agttcggcga ctggcacact
     1801 ccgcaggcgc tctggcacag gaagacggca gcccacaaca ccgttgttgt cgacggacgc
     1861 gaccaggctg gtggacctgc cgaatgcact ctgtggaacg cgggaggtcc gctgcaagcg
     1921 attcgcgcgt cctcgccttc gcagatcaaa ggctccgtct atgagcgcac catcgccatg
     1981 atcgaaaccg gccccaccgg ctattacgtt ttcgatgtgt tccgcgttca gggcggcaag
     2041 gaacatgcca agcacactca cacgacgttc gccgcgccca ccgtgttcgg cgtccggctc
     2101 acaccgtcac cgccgctcta cgattcgggc acattgatgc gcgcgtttca gaccggccgc
     2161 gccggaccat cctggggcgt ggattggaag atcgaagaca agcacggcta tgtccagccg
     2221 ccgcgcgatc ttcatcttcg ctataccgac ctcactcgcg atgcggaagc atatacggcc
     2281 gagagctgga ccgtcgccag cgccacttcg accaccgaat actggattcc caccgcgatt
     2341 gtgcgccggc gctcggatag cggcgatttg acatctactt ttgtgggctt gctcgaccct
     2401 tatgaaggcc agccgcagtt gaagcgcatt acgcgcctcg accgggatga cgcggccgtg
     2461 caggtggaag tggaactcag cggcggtacc cgcgaccggc tcctttcaaa tgccgcaggc
     2521 gtcacactgg aacgccgcga cgccagcggc aaagttatcc ttacggcctc agccatcggt
     2581 cgataaacgc ataggcccgt tccgacgctt ccggcggaaa cccgtggccg ccgcgaaaga
     2641 aatagctgtc cagccggtcg cccgcgccca gaagctgata caccggatcg agccttgcca
     2701 aagcctggcg ggcgctccaa ccggcgttcg gaaagatcga atcttcgtcc gaagtggaca
     2761 gaaacagcgg acgcggcgcg atcatcgcca gaacataatg cagatcgagt ggagtctgca
     2821 cggtttccga atccggcaca ccggcataga acccattctt gagatacggg ctcgagaggt
     2881 agaaaccgag ccgcggcatc aacgccgtgg cgtgcgacca gcgccatgta ttgccgtcat
     2941 ggcggaacgt gtcgaacccg caactgctta ctcccaccgt gatgcgcggg tccagtgcca
     3001 tcgcgaacag agtggtgtag ccgccgtgcg aatgaccaat ggaggcgatg cgcgcggagt
     3061 cgacaaaatc gagcgtctga agatagtcca cagcccggct caggtcccag gccatcttgc
     3121 ccatcatcga ccagcgcgga tgtttgcgat agaacggaat cgcctcgccg tagtggcctg
     3181 tggagggatc gtgcctttct ccgaaacacg cggcatcgta ggtgaaggtg acgtatccac
     3241 ggtttacaag ctccaacgcc atatgcagcc gcggattgcc ggccagcccg gccggttcgt
     3301 ctttgccaaa ctgcaccgtc tgatgcgggc agaccacggc cggtttgcgg ctttcgagtt
     3361 tgtgtggcac cagcaaatag gcgggcacga actcaccagg ctccgcctga tagaccagct
     3421 tccggcgtat atgcgtggga agcttcacct cctccaggat tcgcggcgcc agaggcggct
     3481 tgttttccgg cggcgcaccg agaatggcga gaaccgtctg ttcgatggag acgcggcgtt
     3541 cgggccaatc gccgcgcgca accgcctttc cgccttgaaa ctggagcaag tcagcgaggg
     3601 ccgcctggcc ggtctgctcc gttcgtagta ccggacgatt ctcgccggtg atctcccgcc
     3661 agcgctccca actccaggag acatacggac tgcgcgcctg catggcaacc atcgggagag
     3721 atagaaagtt ccgccgttcc atccctcgaa ttgtataggc gccggctgta aaacggcaca
     3781 tcaaaaccag gcaaaccagc gagggaagtt gctatcgtcg tggatgactt ccgtgttctg
     3841 gtacctgttg gcggccgcgg gggagatcgc cggctgcttt accttctggg catggctgcg
     3901 gctgaataag agcccgctct ggctccttcc cggacttgtt gccctgatcg tcttcgccac
     3961 cgccctgacc cgcatcgaga cagcgaacgc cggccgcgcg tacgcggcct acggaggcgt
     4021 ctacattctg tcctcattgc tttggctttg ggccgttgag agcgtccgcc cggaccggtg
     4081 ggaccttctt ggtgcgtcga tttgcctggc tggcgcggcc gtcattctct tcggtccgcg
     4141 agtggtctga acatgctcac cggcggtcgc ggctcagcat tcgtttcaga gccacgaccg
     4201 ccagggaggg gtcaatcaga agtaaatctt cgcgccgaat tggatgattc gcatcgggtt
     4261 gcgcaggctg cggatttctc cgttgcgcgg gccgtcgggg atcaccgaat tgagtgacgt
     4321 gtaactgatg ccgcccggct gtccgaagtt tggatggttg aacaggttga aaaactcggc
     4381 ccggaattgc agccggccct gctctcccag cggcagccgc cagttcttgt acacggagaa
     4441 atcgacgttc tgcccgccgg ggctgaccat ggcgtccatc gccgcattgc cgtaatggca
     4501 aaggtcggga cggccgttga tgttgcaggc ggtgcgccgg aatgcagtga tgtcgtacca
     4561 catctggcgg gtggctgcgt ctcccaaacg gccgtcctct acccggtccg gcaatgtaaa
     4621 cccaccggtg ttcaagtcgc tgccgccggt gacggtgaac ggaaatcccg tccgaagggt
     4681 gactacgccg ttcatctgcc agccgccgat gaatgctccc ggtacgccgc gaatattcct
     4741 gaggaaaggc atctcgtaaa cgaagtttac caccgcgttt tgtgtgacgt cgaagccgta
     4801 gcgcgcccgc gcctgccgcc ggtcgcgggg gttctggtaa ccgttgctga cgcccgcgcc
     4861 gccttcgtta cgcccatagc cctctccaag cgctttgccg tacgtgtatg ccaggccgag
     4921 aaccaggccg gcgctgtcgt aacgcttatc tacgctcacc tgcagcgcgt tgtagttccc
     4981 gttgccgtag ctgtcgaggt aacggatgtt gccggtgccg aacaccgccg ggttgccgtt
     5041 cgcgtccacc tcgccttcgg agatatggaa cggatagggc cgtcgcgcct ggatatttgt
     5101 gtccagatat gccgggtcgg gattattggc cgtgatcgtg ttatccaggt ggtttgtttt
     5161 gctgccgatg tagccgaccg tcagcactgt gttccacgga aggttgcgtt gcagatcgag
     5221 actccactgc cagtgattcg acgacctgtt atccggcggc acaagcacgg tgttgcgaac
     5281 agaggagacg ctgccgctac ccgcgccata gatgttgccc acctggttgg caaagcccgg
     5341 acgcagacgg ttggtctgga tctggtacgt ccgcccggca tactcatagc tgaacggcgt
     5401 caggatgtcc tgctgaatct gataagcgat ggtgccggat accggaggca ccaggttcag
     5461 aatcgtgtag ttgttcagtt gctgtgcgtt ggcgaaccaa cccgctccgg cgcgcattac
     5521 ccacttgtcg gtggcgcgat aggcgatacc gacgcgcggc atgtaatagc ggttttccct
     5581 gttgtagggc gtccagttct tcagacccac ttcggggatg acggtcggaa gttgccgccc
     5641 gtccgccgcc cggctcagga tatcgagccg gagagaacgc caggcgccgg cgctgtcgcg
     5701 cggggtttga aagaagtccc agcgcatgcc gaggttgatg gtcagcttgc gcgtcgcttt
     5761 ccagtcgtcg aggaaatagg cggaccagcg gttctgccgc ggcgctgtga acggaaaacc
     5821 ctcgccggtc tccgtgttgc tgaagtaccc catcagccag ccggcaagac tgttcccacc
     5881 ggggcagcac gcgatgctgc cgcgcggcac attggccgcc gcgcggtcca ggatcagtcg
     5941 ccggtactcg aacccggctt tgaagttgtg cggcccgcgt gtaaacgaca ggttgtcggc
     6001 gaactggtgc accgtgttgc gatcccatcc gtttccaccg tcgcggtcac cggctatgat
     6061 cgtcgagggt atacccactt cccgatccgt cagcttgcgc ccgctggtga cgcggaacga
     6121 acccaggccc agcgaatcga ggtcgaaacc ggtgttagtg cgcggattga atgtgtcgtc
     6181 gtcggccttg ttgattccgt aacggaattc attcaatgtg gttggcgaga ggatcttcac
     6241 gtattgcgtc gccagattcc agttcagcgt ctggacaaag taggtgaaat tcgggttcag
     6301 agcgccgttc gtccactttc cccggtccgt aatgtatcgc acaaaaacct tatcgctcga
     6361 cgagaagtta tgatcgatgc gaaacatgta ctggttcgtg ttgatgatgt tgggcactga
     6421 cccacgggca ttaatatcca acacatcggc tggctggaac atcggcaaag gctggtacgt
     6481 gttgataaaa tcctgcgtgg ccttgttgat ccggctggcc gggatgatat tcgtaatccg
     6541 cccggagctg tcgcgaaagg gctcgcctgt ggcgggatcg aaaatgatgg taggcgaccg
     6601 gatcggcgcc ccgttcggcc caataggcgg cgtcaggagt gccgagaaat cgccgcggcg
     6661 aaatgcctcc ggaaaccagg ccgcttcttc gacggtctcg cgcgtctcgc gccggccttc
     6721 atagttgaag ctccagaacg tcctgtcttt gccgttgtaa attttcggca gtattaccgg
     6781 accgctgacg aacgtaccga actggttgcg ccgcagaacg cttttcgaac cttcgcgagc
     6841 gcctgcgggt aactggaagt tcaggaagta gtccttcgcc gcgagtttgt cattgcgcag
     6901 aaactcccag aaggtgccgt gcaggttatt ggttcccgcc ttcaccacca tctgcaggat
     6961 tgctccattg ttctgcccgt attcggcgga gtaggacgac gtttgcacct tgaactcttc
     7021 aagtgcatcg atggaaggag aaaacgcggc ggtgttcacg cgcggttcgg ttgcgatcac
     7081 accatcgagc gtcacctgct ggttcacctc gcgttgccca ttcgcgctga tggcgacgcc
     7141 gttgcccgga atcgggaagc cgcccgttcc atccagcccc attcggatgc cgtactgaac
     7201 gcctggcacc aacacggcaa gtgtggccac attgcgtcca ttcagcggaa gctccacgac
     7261 gcgcttgtta tcgatgttgc cgcccaccgc cgcatcttcc gtctttaacg cgactccgct
     7321 ggcgacaact tcgatggttt ccgttgtggc gccgatctcc atccggaaat cgatacgcgc
     7381 cttctgttga agctgaacgg tgatgcctct ttgctcctgc gttttgaatc cctcccgttc
     7441 caccttgact gtgtagtcgc cgacttcgat caacgggaac acgtagttcc cgtcgtcgtt
     7501 cgtcaaggtt tcacgagcct gtccggtggc ggtgcgcaag agcgtgactc tcgcgctggg
     7561 aacggaagct cccgccggat ccatgaccgt gcccagaatt tcggttgacg tagtttgtgc
     7621 gaggacccca gcggccgcta cacaaaggga aactgacaga acggcaaacc tcattatctc
     7681 cccctctagc cagataagtt gcacccagac tggataggta gaaagtaacc gaagattagc
     7741 ggactgtcaa gctatccggg cataatattc gcaagtgaaa ctcgcccggc tcgcgcggcc
     7801 aaatatgtcc tcaatagtac attttctact tgaaatgtag taaggagaat cagttaggag
     7861 gcaagttact aagccttcac gcggtaccgc tcgattgcgt ctttatccag atccacgccc
     7921 aggccagggc ggtccgatac cgttgcgtag ctgtcccgaa tgtcgatggg ttcaacaatg
     7981 aggtcgtcct cgcgcaggaa actcaggatg tcgctgggca tggtgcagtt cggcgatgcc
     8041 gcgcaggagt gaaggtacga tgcatccacg atgccgcaat cgttgcccga accgtgccat
     8101 accggaatgc cggccgcgcc cgcgagatag cagttcgcga cgaagccgct catccccggg
     8161 ttcccgcccg tgttaaaaat ggagcaggca tcttccttga tcgctcgaat catcgccttc
     8221 gggtctccca ggtgcaacgc cacatgtgtt ttcacttcgc ggcgcagctt gcggtaacct
     8281 tcgaaatcgt tcttgtcgaa cgggtcttca aagaccagcg cattgccgat ggcatccagc
     8341 gctttcgcaa cgtggagaaa atcggcgtaa cttttgtacc gggtgttagg gtcgacgatg
     8401 tacttgatgc cgggcgcatg cttggccagg atctctgccg tttccaccac cggatcgccc
     8461 tgcgcacact ttgtctttac agaggtgaac ttgccggcga cggcgcgttc ggcgacgcgg
     8521 atcatccccc tcggcgtacg ccggcctgtc caatagccga ccagcacctt gcgctgtgcc
     8581 agaccgccca gcagcttgta tacgggccag ccgaaagcct tgcccacaat gtcgtatagc
     8641 gccatttcga atgcagcata gccggccctt accggcagat ccagacgagt caggttgaaa
     8701 tcgagcgcct tccttccttt cagaaacgtg gcgttctttt gcacggccgc gtaatcct
//
LOCUS       DASTMK010000167         8462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_232410, whole genome shotgun sequence.
ACCESSION   DASTMK010000167 DASTMK010000000
VERSION     DASTMK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232410"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFQ79_07220"
     CDS             <1..240
                     /locus_tag="VFQ79_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU0139481.1"
                     /translation="AGREEVFAFMDRLRLVVRATSLGDVHTMILYPAMSSHRDLSPKQ
                     RERIGIHDNLLRLSVGIEAVEDIIADFEQALGGQG"
     gene            complement(246..698)
                     /locus_tag="VFQ79_07225"
     CDS             complement(246..698)
                     /locus_tag="VFQ79_07225"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0139482.1"
                     /translation="MSSVFLLGRLLFGGYFLYNGINHFRNHESLTQYAAAKDVPNPDA
                     AVSGTGALLLLGGASIVLGLKPKLGAAALTMFLAGVSSMMHDFWRVEDSGQRMNETIN
                     FTKNVALLGAALALMGTEEPWPASLPHCPRHAGRLTGESRPPSSPVPV"
     gene            923..2251
                     /locus_tag="VFQ79_07230"
     CDS             923..2251
                     /locus_tag="VFQ79_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139483.1"
                     /translation="MQRDERATRRDFISIAALSAAGAAISTTSAQGQTTKRLRVAIVG
                     TGGRGIGTWGRPVASEYADVVEIVGLCDINSKRAKVAQSQIGTDAPIFSDFDRMVRET
                     KPNAVIVTTVDATHYRYTVRALELGCDVITEKPMCTDEEQCQAILDTQKRTGRKVRVT
                     FNARHSPEAKKLKSLLMEKAVGEIVSVDFHEYLDTSHGADYFRRWHHLKENSGTLLVH
                     KASHHFDQANWWLDSQPVEVTATGELKFYGKNNSFRGTHCRACPYKQECKFYWDITRN
                     ANAVRLYVDCESEDGYLRDACLWRENTNIYDTMSVIVKYENGVLLTYTANTYLPYEGQ
                     AIAFNGTDGRLDLNEFRGGGFKTNEIRLTRSFGKSEVVRDLEARQRGGHGGADTGIRD
                     LMFRNQDGPDPLNLRADLRAGALSSLIGIAAYRSIERGGVPVKIDSLVKL"
     gene            2273..3436
                     /locus_tag="VFQ79_07235"
     CDS             2273..3436
                     /locus_tag="VFQ79_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0139484.1"
                     /translation="MYRSAIIPMTALLLMGVSCSQAPDAASKEETGKAKGGAEELKYG
                     IRVQSGPMDSILLKDYKPASSLVVPETMVPKAKYPVIDIHSHTFMNGIKTSEDVAAWV
                     RTMDEVGIEKSIVFTGATGADFDKQVELFKPYASRFQLWCDLDTTNIDAPDYPERAAA
                     ELERCYRMGARGIGELSDKGSGLQSEKRPRGKRLHYDDPRLDLLWKKCAELKIPVNFH
                     VADHPSCWQPLGPNQERTPDFQHFNLLDKDVPSYEELIAMSDKMLEKNPKTTFIACHL
                     RNQGNNLGELSKAMERYPNLFLDISARDYEIGRQPRAAAKFLTRYRNRVMFGTDMGRG
                     KEMYQGWWRLLETADEYLPGRVWWRIYGLELTPQVLESLYRSNALKILNWEKL"
     gene            complement(3450..3908)
                     /locus_tag="VFQ79_07240"
     CDS             complement(3450..3908)
                     /locus_tag="VFQ79_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139485.1"
                     /translation="MKIRTGVYRFAVLAAGMLLFSGLLVAGEKKLMHCFYFTAIESAT
                     QADWDAFHKATDQLTKQIPGLTRVWHGKLIRPMAQFNPDAETRKKFDDQTNSATGQFS
                     RRIRGHGVCMEMNDQQTLKTYANHPAHKEWEQVYAKVRQPGTMTFDLIGE"
     gene            complement(3944..4639)
                     /locus_tag="VFQ79_07245"
     CDS             complement(3944..4639)
                     /locus_tag="VFQ79_07245"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0139486.1"
                     /translation="MRNIFLVFCAGLFALGITVLSAQKSGDYRDPDYQYSEDWKVLFN
                     GKDFTGWVPILRTPEGKARRYRENETGDQNTFYIKDGLLMTTGTPNGYVRTTDVYDNF
                     VFHVEVRFPQSGNSGVLIYVPRDDVWPKGVECQLYQTHMGRIFPIQGATLDGGEMIHA
                     AAKPIGEWNTYEVYSEEGRVATVVNGVLVGLASNANPRVGYICLQSEGVPAEFRNIKV
                     KRYTPAYHLRSDK"
     gene            complement(4686..5372)
                     /locus_tag="VFQ79_07250"
     CDS             complement(4686..5372)
                     /locus_tag="VFQ79_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU0139487.1"
                     /translation="MADDTERKRFAVIGLGHFGAYVARSLYESGKEVLAVDSSAEVVK
                     DATEWSTEAVVADATDRETLEAIGLAEVDAAIISLGERMDVSTLAALHVKELGVSYIV
                     VKALSEDHGKILTALGVHEVIHPEKDMALRLASRLARTDVIEVLPILPGYSIREIRAP
                     SEFVGRSLRDLAIRNTLHVQLIAIQSGDGEHRRMNIVPRADDVIQEGDLMVLLGEDRD
                     LDRIREILAR"
     gene            complement(5372..6796)
                     /locus_tag="VFQ79_07255"
     CDS             complement(5372..6796)
                     /locus_tag="VFQ79_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU0139488.1"
                     /translation="MADATDRRLAIRKIHPARIIAGGFAGVIAMGTALLSLPTATVNG
                     TRIPLIDAMFTAVSATCVTGLAVVDTGTVFSRFGQAVILACIQIGGLGLMTMTTVFLV
                     AFGRRLAITDRIAMQDSFHHTRDPQLGALIKYIAAATFATEGLGAAILSIYWIATGRF
                     ESPWTAVYHAVFHAVSAFCNAGFALYSDNLTGFQSDPVVLMVICGLIITGGLGFLVGL
                     DVTTCLRQSFLLRFSPPGRREHAMPRLSLHSKLVLTGTAALLAAGMVSYYLLERRALF
                     ARMGEFEAWLNAFFCSVTARTAGFNTIDYSDMSSPALLCTIVLMFIGASPGSTGGGIK
                     TSTFVLLIAYAYSRWRGNEDLHVFNRRVPQETLDKAQSVVFAALATFVLGGSALVGIE
                     AGAMSPAASHDQFLSLIFETTSAMGTVGLSMGQTPLLSAPGKLILMLLMFMGRIGPLT
                     MALAISIRRTRSRFRYADENVMVG"
     gene            complement(6796..8196)
                     /locus_tag="VFQ79_07260"
     CDS             complement(6796..8196)
                     /locus_tag="VFQ79_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEU0139489.1"
                     /translation="MRRGGPPALHPARIIAVSFAAVVAMGTALLSLPAATVSGTRIPF
                     IDAFFTAVSATCVTGLLALDTSTVFSPFGQIVILACIQIGGLGLMTLTTIFMVAFGRR
                     LAIADRTAIQDSFHHSRSPRLGTLIKYIVAGTFATEAAGAAILSAYWLATHRFDSAGT
                     AIYHAVFHSISAFCNAGFALYTDNLVGFRDDPVVLAVISGLVIAGGLGFLVGLDLKDY
                     LQQKYLLRGVSPGTRPVARLSLHTKLVLIGSSALLAAGTVSYYLLERKALFAEMSGFE
                     AALNAFFCSVTRTAGFNTVDYARMSSPSLLCTMVLMMIGASPGSTGGGIKTSTFVLLV
                     AYAYSRWRGNEDLHIFRRRVPQEVLDKAQSVVFVMLAAFVLALSALIGVEAQGIAPAE
                     SHKQFLQLSFETASALGTAGLSMGQTPLLSMPGKLILMLLMFMGRVGPLTLALAISIR
                     RSRSRFRYADENVMVG"
     gene            complement(8193..>8462)
                     /locus_tag="VFQ79_07265"
     CDS             complement(8193..>8462)
                     /locus_tag="VFQ79_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139490.1"
                     /translation="NPSEQMYDIRKDPGCLHNLADTPSHATIRTRLREQLDRRLLAEK
                     DPRATGSGDIWESYPRYSPMRPQLGGFAEMGKYNPKYQQARRPPV"
BASE COUNT         1873 a         2443 c         2511 g         1635 t
ORIGIN      
        1 gcggggcgcg aagaggtctt tgcctttatg gaccggttgc ggctggtagt gcgcgccacc
       61 tctctgggcg atgtgcatac gatgattctc tatccggcga tgagctctca ccgcgacttg
      121 tcgcccaagc agcgggaaag gatcggcatc cacgataatc ttttgcgcct gtcggtggga
      181 atcgaggcgg tggaggatat tatcgcggac ttcgaacaag cccttggcgg ccagggctga
      241 gcatcctaca ccggcaccgg tgacgacggc ggacgagact caccggtcag ccggccggcg
      301 tgccttggac aatggggcag gcttgcgggc cagggctctt ccgtgcccat caatgccagg
      361 gcggcgccga gtagagccac gttcttggtg aaattaatag tctcgttcat tcgttggccg
      421 gaatcttcca cgcgccagaa gtcgtgcatc atggacgaaa cgccggccag gaacatcgta
      481 agagccgctg cgccgagctt cggcttcaag ccgaggacga tactcgcgcc gcccagcagc
      541 aggagggcgc ctgttccgct caccgcggca tcggggttgg ggacgtcctt tgccgcggcg
      601 tattgggtca ggctctcatg gtttcggaag tgattgattc cattgtagag gaagtagccg
      661 ccaaacaaga gccgcccaag taaaaacaca gagctcataa aagtcacctc ccgtttgtaa
      721 gttcctgtca ttggactcgg ccgccggggg atgtgtttca tgctcccgcg ggtatctttc
      781 atgcagcggc atgcgcccgc cagggagcgg ttgcgcaatc atttctgacg ccacttttaa
      841 gtcacttaca gacctgtttc tcttgtatcc ttgaagggga tgagccgtgc gacgcggact
      901 cattcaccag gagaatacct tcatgcagag agatgagaga gcaacccggc gggacttcat
      961 cagtatcgct gcgctgtcgg ctgccggcgc ggcgatctcc accacgagtg cccagggaca
     1021 gaccacgaag cggctgagag ttgcgattgt cggaacggga gggcggggga ttggtacgtg
     1081 gggcagaccc gtcgcctccg agtatgcgga cgttgtcgaa atcgtcggcc tgtgcgacat
     1141 caacagcaaa cgggcgaagg ttgcgcagag ccagattgga acagacgcgc cgattttttc
     1201 cgatttcgat cgcatggtca gggagacgaa acccaacgcg gtgattgtga ccacagtgga
     1261 cgccacacac taccgctata cggtacgcgc gctggaactc gggtgcgatg tcatcaccga
     1321 aaagcccatg tgcaccgacg aggagcagtg ccaggctatc ctggacaccc aaaaacggac
     1381 cgggcgaaaa gtgcgggtca ccttcaatgc ccgtcattcg ccggaggcaa agaagctgaa
     1441 gtcgctgctg atggagaaag ccgtcgggga gatcgtgtcc gtggatttcc acgaatacct
     1501 cgatacttcg cacggcgcgg actacttccg gcgctggcac catctgaaag aaaattcggg
     1561 cacgctgctg gttcacaaag cgtcgcatca tttcgaccag gcaaactggt ggctcgattc
     1621 acagccggtg gaagtgacgg ctaccggcga gctaaagttc tacggcaaga acaattcgtt
     1681 tcgtggcacg cactgccgcg cgtgtcccta caagcaggaa tgcaagttct attgggacat
     1741 caccaggaat gcaaacgccg tccggctgta cgtcgattgc gagtccgagg atggctatct
     1801 gcgtgatgcg tgcctgtggc gtgaaaacac aaatatctat gacaccatgt ccgtgattgt
     1861 gaagtatgag aacggagtcc tgctcaccta cacagccaat acttatctgc cttacgaggg
     1921 gcaggccatc gcgttcaacg ggaccgatgg acgcctggac ctgaacgaat ttcgaggcgg
     1981 cggattcaag acaaatgaaa tccgcctgac ccggagcttc ggaaaatccg aagtcgtccg
     2041 cgacctcgaa gcccggcagc ggggtggaca cggcggggcc gacacgggca tccgggacct
     2101 gatgtttcgc aaccaggacg gtccggatcc gctcaacttg cgggcggatc tccgcgcggg
     2161 cgctttgtca agcctgatcg gaatcgcggc ctaccgcagt atcgagcgcg gcggagtgcc
     2221 tgtcaaaatt gatagtctag tcaagctgta actacaaacc tggaaggatg acatgtatcg
     2281 atcagcaatt attcccatga ccgctcttct gctgatggga gtttcctgca gccaggctcc
     2341 ggacgctgcg agcaaagagg aaaccggaaa ggccaaagga ggcgcggagg agctcaaata
     2401 cggcatccgg gtgcaatccg gaccgatgga ttccatcctg ctgaaggact acaagcccgc
     2461 ttcctcgctt gttgttccgg aaacgatggt gccgaaggcg aagtatccgg tgatcgacat
     2521 ccacagccac acgttcatga acggcattaa aacttccgag gacgttgcgg cctgggtgcg
     2581 aaccatggac gaggtcggaa tcgagaaatc gattgttttc acgggagcca ccggtgcgga
     2641 tttcgacaag caggtggaac tgttcaagcc gtacgccagc cgctttcagc tctggtgcga
     2701 tctggatacc actaacatcg atgcgccgga ctacccggaa cgcgcggcgg cggagttgga
     2761 gcggtgctac cgcatgggcg cgcgcggtat cggagagttg tcggacaagg gctcgggact
     2821 acagagtgag aagcgccctc gaggtaaacg gctccattat gacgatccgc ggctcgatct
     2881 gctctggaag aaatgcgccg agctgaagat acccgtgaat ttccacgtgg cggaccaccc
     2941 atcctgctgg cagccccttg gcccgaatca ggagcggaca cccgattttc agcacttcaa
     3001 tctgttggat aaggatgtgc cttcgtatga agagctgatc gcaatgagcg acaagatgct
     3061 ggagaagaac ccgaaaacga cgttcatcgc gtgccatttg cggaaccagg gcaacaacct
     3121 tggggaactc agcaaggcga tggagcgtta tccaaacctg tttctcgata tttcggcgcg
     3181 cgactacgag attggccggc agccgagggc agccgcgaaa ttcctgacac ggtaccggaa
     3241 tcgcgtcatg ttcgggacgg acatgggtcg aggcaaggag atgtaccagg gctggtggcg
     3301 tctgcttgaa acagccgacg aatatctgcc aggccgtgtc tggtggcgga tctacggcct
     3361 tgaactcaca ccacaggttc tcgaaagcct ctatcgaagc aatgcgctaa agattctgaa
     3421 ctgggaaaag ctgtagccgc ttaccgcggc tactcgccga tgagatcaaa agtcattgtg
     3481 cccggctgcc gcactttggc gtagacctgc tcccactctt tgtgggccgg gtggttggcg
     3541 taggtcttca gtgtctgctg gtcgttcatc tccatgcaga cgccatgacc gcgaatgcgc
     3601 cgcgaaaatt gtccggtggc gctgttcgtc tggtcgtcga acttcttgcg ggtttcggcg
     3661 tccggattga actgcgccat gggacggatc agtttcccat gccagacacg agtaagcccg
     3721 ggaatctgct tcgtaagctg atcggtagct ttgtggaaag cgtcccaatc ggcttgggtg
     3781 gcgctctcga ttgcggtgaa gtagaaacag tgcatcagtt tcttctcgcc ggccacaagc
     3841 agcccggaaa acagcaacat gccggccgcc aggacggcaa agcggtacac acctgttcgt
     3901 attttcatcc agtcctcccg tgccagaaat tctatcagac ggatcactta tccgaacgca
     3961 ggtggtaagc cggtgtgtaa cgcttcacct tgatattgcg gaactccgcc ggcacccctt
     4021 ccgattgcag gcaaatatat ccgacccgcg gattcgcgtt cgacgccagg ccgaccagga
     4081 cgccgttcac caccgtcgct acgcgtcctt cctcggagta aacctcgtag gtgttccatt
     4141 cgccgatcgg cttggcggcg gcgtggatca tttcgccgcc atcgagcgtg gctccttgaa
     4201 taggaaagat gcggcccatg tgggtctggt acaactggca ttcgacgcct ttgggccaga
     4261 catcgtcccg cggtacgtaa atcagcaccc cgctgttgcc gctttgcgga aaccgcactt
     4321 cgacgtgaaa aacgaaattg tcgtacacgt ccgtggtgcg cacgtacccg ttcggcgtgc
     4381 cggtcgtcat gagaagtccg tccttgatgt aaaacgtatt ttgatcaccg gtttcattct
     4441 cgcggtatcg cctggctttg ccctcgggcg tccttagaat cggaacccag cctgtgaaat
     4501 ctttcccgtt gaacaggact ttccaatcct cggaatattg gtagtccgga tcgcgataat
     4561 cgccactctt ttgagcggac aggacggtga ttcccagggc gaagaggccc gcacagaata
     4621 caagaaatat gtttcgcatc ggacctatta caacagaaga gagaccggac tgccagacag
     4681 cgctttcacc tggccagaat ttcccggata cgatccaggt cgcgatcttc gccgagaagc
     4741 accatgaggt caccttcctg gatcacgtcg tcggcgcgcg gaacgatgtt catccgccgg
     4801 tgttctccat ccccgctctg tatggcgatc aactgcacgt gcagagtgtt ccggatcgcc
     4861 aggtcgcgaa gcgaccgtcc cacaaattcg gaaggcgctc tgatctcgcg aatcgaatag
     4921 ccaggcagaa tgggaagcac ttcgatcacg tcggttctgg ccaggcggct ggccaggcga
     4981 agcgccatgt ccttttccgg atggatcacc tcgtgcactc caagagcggt gagaatcttg
     5041 ccatgatctt ccgagagcgc cttgacgaca atgtaactta cacccagttc tttgacgtgc
     5101 agcgccgcca gcgtactcac gtccatgcgc tctccgaggc tgatgatggc ggcatcgacc
     5161 tcggcgaggc cgatggcctc cagcgtttcc cggtcggtgg cgtccgccac caccgcttcc
     5221 gtcgaccatt cggtagcatc ttttactacc tcggcactcg agtcgacggc aagaacctcc
     5281 ttgccggact catagagcga tcgtgcgaca taggcgccga aatgccccag tccaatcacc
     5341 gcgaatcgtt ttctttccgt gtcgtctgcc atcaacccac catcacgttc tcgtcggcat
     5401 agcggaaccg gcttcgggtc cggcgaatac tgatcgccag cgccatcgtc aaaggcccga
     5461 tccggcccat gaacatcaat aacatcagaa taagttttcc cggcgcgctt agcagcggcg
     5521 tctgtcccat cgagaggccg acggtgccca ttgccgatgt ggtctcaaag atcagactca
     5581 ggaactggtc gtggcttgcc gcgggagaca tcgcgccggc ctcgatccca accagtgccg
     5641 aaccccccag aacaaaggtt gccagggcgg cgaaaacgac cgactgggcc ttatcgagcg
     5701 tctcttgtgg aacgcggcga ttgaacacat gcagatcttc attgccacgc catcgcgagt
     5761 aggcataggc gatcagcaat acaaaggtgc ttgtcttgat gcctccgccg gttgagcccg
     5821 ggctggcccc gataaacatc agcacgatgg tgcagagcag agcggggctg ctcatgtccg
     5881 aatagtcgat ggtgttgaag ccggccgtcc gcgccgtgac cgaacagaaa aacgcattga
     5941 gccaggcttc gaattcaccc atccgcgcaa acagggctct gcgttccaac agataatagg
     6001 aaaccatccc ggccgcgagc aacgccgcgg ttccagtcaa caccagtttc gagtgcaggc
     6061 tgagccttgg catcgcgtgt tcccgcctcc caggcggcga aaagcgaagg agaaaactct
     6121 gtcgcaggca ggtggtgacg tcgagcccca cgagaaagcc caggccgccc gtaatgatca
     6181 gaccgcagat caccatcaat accaccgggt cgctctgaaa accggtcaga ttatcgctgt
     6241 agagtgcgaa gccggcatta cagaacgcgg aaacagcgtg aaagacggcg tgataaacgg
     6301 ccgtccaggg cgattcaaaa cggcccgttg caatccaata gatggataga atcgcagctc
     6361 ccaaaccttc cgtcgcaaag gtggcggccg cgatgtattt aatgagcgca cccagttgcg
     6421 gatcgcgggt atggtggaag ctgtcctgca tggcgatgcg gtccgtgatg gccagcctgc
     6481 gcccgaatgc caccaggaaa acggtagtca ttgtcatcag gcccagcccg ccgatctgaa
     6541 tacaggcgag aatgacggcc tggccgaagc gcgagaagac tgtgcccgtg tcgacgacgg
     6601 ccagtccggt tacacaggtg gcggaaaccg ccgtaaacat cgcgtcgatg agaggaatac
     6661 gcgttccgtt caccgtagcg gtggggagag aaagcagcgc ggtccccatt gcgataacgc
     6721 cggcaaaacc tcccgctata atccgtgccg gatgtatctt gcggattgcg agacgtctat
     6781 ccgtggcgtc cgccatcaac ctaccatcac gttctcgtcg gcgtaacgga aacggcttcg
     6841 agaacggcga atactgatcg ccagcgccag tgtcagaggg ccgacccgtc ccatgaacat
     6901 caataacatc agaatgagtt ttcccggcat gctcagcaga ggtgtctgcc ccatggagag
     6961 cccggccgtt ccaagagccg atgcggtttc aaaggagagc tgtaaaaact gcttgtgact
     7021 ctccgcgggt gcaatcccct gggcttccac tccgatcaga gccgaaagcg ccagaacgaa
     7081 ggcggcgagc atgacgaaaa caaccgactg cgccttgtcg agaacttcct gcggaacacg
     7141 acggcgaaaa atatgcagat cttcgttgcc gcgccatcgc gagtacgcgt aagcaaccag
     7201 cagcacgaag gtactcgtct tgatgccgcc gccggtggaa ccggggctcg cgccgatcat
     7261 catgagaacc atcgtgcaga gcagggaagg gctgctcatc cgggcgtaat cgaccgtatt
     7321 gaacccggcg gttcgtgtca cggaacaaaa gaacgcgttg agggccgctt cgaatccgct
     7381 catttccgcg aacaacgctt tgcgttccag caggtaatag gaaaccgtgc cggccgctag
     7441 cagagctgag gacccgatga gcaccagttt cgtatgcagg ctcaaccttg ctaccgggcg
     7501 agtaccgggc gataccccac gcagcaaata cttttgttgc agataatcct tcagatcgag
     7561 gccgaccagg aaacccaggc cgcccgcaat gacaaggccg ctgatgacgg ccaagaccac
     7621 cggatcgtca cggaacccaa ccagattgtc ggtgtacaga gcaaacccgg cattgcagaa
     7681 tgccgagatc gaatgaaaaa cggcgtgata gatcgccgtg ccggccgaat cgaaacgatg
     7741 ggtcgccagc cagtatgccg acaggatggc ggcgcctgcc gcctccgtcg caaaggtgcc
     7801 ggccacgatg tatttgatga gcgtgcccag gcgcggactg cggctgtggt ggaagctgtc
     7861 ctgaatggcg gtgcgatccg cgatcgccag tctccgccca aaggcgacca tgaaaatcgt
     7921 agtgagtgtc atcaggccca ggccgccgat ctgaatgcag gcaagaatga cgatctggcc
     7981 aaaaggcgaa aacaccgtgc tcgtatcaag tgcgagaagg ccggtgacac atgtcgcgga
     8041 aaccgccgtg aaaaacgcgt cgatgaatgg aattcgggtg ccgctgacgg tcgcggccgg
     8101 aagcgaaagc aaagcagttc ccatcgcgac cacggcggcg aaacttacgg caataattcg
     8161 cgccgggtgc agtgccgggg gtccgccacg cctcatacag gcggacgccg tgcctgctga
     8221 tatttagggt tgtatttccc catttccgcg aacccgccga gttgcggacg catcggcgaa
     8281 tatcggggat agctttccca aatgtcgccg cttccggtgg cacggggatc cttttccgcc
     8341 agcaggcgcc ggtcgagctg ctcgcgtagc ctggtccgga tcgtcgcgtg ggacggtgtg
     8401 tccgccaggt tgtggagaca gcctggatcc ttccggatat cgtacatctg ttcggacggg
     8461 tt
//
LOCUS       DASTMK010000168         5551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_456139, whole genome shotgun sequence.
ACCESSION   DASTMK010000168 DASTMK010000000
VERSION     DASTMK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456139"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFQ79_07270"
     CDS             complement(<1..1163)
                     /locus_tag="VFQ79_07270"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEU0139491.1"
                     /translation="MFSTFSQAAPETIWKAGTAKISIAPGEPVWMAGYAARTRPSEGV
                     ALPVRAKALALEDTSGKKSVLVTADLLGFTARLSQAVAKRAQAKYGLPRERILFNASH
                     THSGPVIDRQLAVAYLIDDKQWAAVDHYTKALEDKLVDVIGQALKNMQPASLSFGRTK
                     AGFAANRRKAFGPGPVQHEVPVLRISGKDGKLLAVVFTYACHNTTLGADFCKLHGDYA
                     GVAQKEIEDRHSGAVAYFVTGCGADANPNPRGTLDHVNRYGSELATAVTEKISGDLRA
                     ISGPLRTAFHTEELDFATPPTRDDLKKKLSHENVYERRHAEEMLKIMDRDGRLPTKYS
                     YPLQVWQFGPDLTLIAMAGEVVVDYNLRLRKELGAENTWVAGYSNDVSAYIPSL"
     gene            complement(1203..3356)
                     /locus_tag="VFQ79_07275"
     CDS             complement(1203..3356)
                     /locus_tag="VFQ79_07275"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEU0139492.1"
                     /translation="MKLFFALLATLMLAGSATPQKPWWEQGPLRIIDLTTSISRIDYR
                     DPALLARQKAEQGYDTEHLDIMQMPGGLDDQGFFFVSKVAGKSNPDYLRKYIAEAKKQ
                     GIRVFIYFNVHWYTTKFGQQHPDWRQIRENGKPLDGVYDTGTDFCVNTPWRKWCFQVL
                     RDLAAYPIDGIFYDGPVYRPDTCYCKYCREKFRAKYGADMPSKQVRRGREFKNLVDFQ
                     AASLADFLRDSNAILKSISPHLALYMNGGVRGSNWATGRLNRVLVPEQDLLGSEGGFI
                     SGDLTRVPLWKPGLTARLLETQAGGKPTVIFAAASHKPWTFSLLPAAELRLMYADSIA
                     NGASVWFGITPFEFDQPDMDAIAAMNRFVAGNADYYRGTRSEARVALVWSETTANFYA
                     GAGAQLIDIDQIAVSGAGNLETEFSGFAAALARAHIPFDVIDDTTLESEPLDRYSAIV
                     LPNAACMSNGVAARLREYVRNGGNLAASFETSMYDDTGIRRSDYALAEVFGAKFAGKI
                     AGPMRWDFIKPMGKHALLAGAEREMIPAPIYHLQAKATTAAPLANFTVPLAGRYEGVP
                     AVSDDPALLLNRFGRGTVVYSSGDIGATIAGFHTPELLQFVSNVAREFAPPQVELENL
                     PGSVELVVRSQNKGDRLLVHLVNFTGEMTRPIRNVVPLSGGVLKLKQGAGFGSASALM
                     SRSQLPVEKQGDGGLRVKLPQLNEYEVVVLERSTP"
     gene            complement(3356..>5551)
                     /locus_tag="VFQ79_07280"
     CDS             complement(3356..>5551)
                     /locus_tag="VFQ79_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139493.1"
                     /translation="LIDKVPLPNQAGAGPAGTLTNYVVNLPNISDVNRWGVRVDHRFS
                     EKDSIWVNLNYSKGNPYFVAQAYPEGYGSWQNGGYSTQSANITHSHSFSPNTLNEFRF
                     GYLRHASVRQGMNRDFDPLSLFPDLYPVSYGGLPNININGHVAIGDYGGSDLTPQLTP
                     QFINNLTHIRGRHTIKTGFDIANNRAASFPATAGMLSGLANNAGLGRFDFNGRFTNNN
                     PTGAAQPAHGFADFLLGYPNVTYRSTTSPVGLFYSTRYSAFVQDDWQVTPRFTLNFGV
                     RFMVQTVWKERDRTQAQFDFASNQLVVPGDRFPSQALPRLIEAYPITTTAAAGLSENL
                     LETDKNNWAPRVGFAFRPFGGNKTVIRGGAGVYYNTLPVYIGFRQLGFNNPPFLLGET
                     FESDPGRTPSLTLASPFPGAGALSPNPSATAVQRDIRNSESYQWNFTVERELKSNLGV
                     RASYVGNKSTHLPWYNYPVNEPRQAPGAIQARRPYQPWAGILLLASGGDSNLHQLQLE
                     AIQRYSNGLSFQAEYSWTRSLDNVPVVGGPQDPYNARADRGNSDQIRRHIFTVAYSYE
                     LPFGPNKPFLKSNGPLKYVVGGWQIGGITYLRTGPPFGVSFTASQPGWLSSRADQVGD
                     PRLSRSERSISKWFDASAFAVPAPFTFGNSARNMLFAPGDIVFDVSLLKDFSIRERAS
                     IQFRAEFFNMPNHANFGGPGANISVPSTVGRIFGAGDPRQIQFGLKVLF"
BASE COUNT         1101 a         1624 c         1722 g         1104 t
ORIGIN      
        1 agggatggga tgtaagcgga aacgtcattg ctgtatccgg ccacccaggt gttttccgcg
       61 ccgagttcct tccttagccg caggttgtaa tcgaccacca cttcgcccgc catcgcgatc
      121 agcgtgaggt ccggaccaaa ctgccagacc tgcagcgggt aggagtattt cgtagggagc
      181 cgtccgtcgc ggtccatgat tttcagcatc tcttccgcgt gccggcgttc gtacacgttc
      241 tcgtgcgaca gtttcttctt cagatcgtcg cgcgtgggag gcgtcgcgaa gtccagctct
      301 tccgtatgaa aggccgtgcg caacggaccg gagatggcgc gcagatcgcc ggatattttc
      361 tccgtgaccg ctgtggcaag ctcgctgccg tagcggttca catggtcgag agtaccgcga
      421 gggttcgggt tcgcatcggc gccgcacccg gtcacgaagt aggccaccgc gccggaatgc
      481 cggtcctcga tctctttctg cgccacgccg gcataatcac cgtggagttt gcaaaagtcg
      541 gcgcccagcg tcgtgttgtg acacgcatag gtaaacacca ccgccagaag cttcccgtct
      601 tttccactga tgcgcaacac cggaacttcg tgctgtacgg gcccaggtcc gaacgccttg
      661 cgccgattcg ccgcgaaacc ggctttcgtg cgcccgaaac tcaggctcgc gggctgcata
      721 tttttcagcg cctgcccgat gacgtccacc aatttgtctt cgagggcctt ggtgtaatga
      781 tcgacggccg cccactgctt atcgtcgatg agatacgcca ccgcgagctg ccggtcaatg
      841 acgggaccgg aatgcgtgtg ggacgcgtta aacagaatgc gttcccgggg cagcccgtat
      901 ttcgcctgcg ctctcttcgc aaccgcctgg ctcaatcgcg ccgtaaaacc aagaaggtcg
      961 gcggtgacca ggacggactt cttccccgaa gtgtcttcga gcgcgagcgc cttggcgcgc
     1021 accggcaacg ctacgccttc ggatggcctg gtacgcgccg catagcccgc catccaaacc
     1081 ggttcaccgg gagcaatcga aattttggcc gtgcccgctt tccagatggt ttcgggggcg
     1141 gcttgactga aagtggaaaa cataaagagg ctaatcagta acaggcggaa catacagatt
     1201 cctcatggtg tgctgcgttc cagcacgacg acttcatatt cgttcaactg aggtaatttt
     1261 acacggagcc cgccgtctcc ttgtttctcc accggcaact ggctgcggga catcagcgcc
     1321 gaggcgctgc cgaatccggc gccctgcttc aacttcagca cgcctccgga aagcggaacc
     1381 acgttgcgta tcggccgcgt catttcgccg gtgaaattca ccaggtggac cagcagccgg
     1441 tcacccttgt tctgcgaacg caccacaagt tccaccgatc cgggcaggtt ttccaactct
     1501 acctggggcg gtgcgaactc acgcgccacg ttgcttacga actgcaacaa ctccggcgtg
     1561 tgaaaaccgg cgatagttgc gccgatgtcg ccgctcgaat agactaccgt tccacgcccg
     1621 aaccggttca gcagcaatgc gggatcgtcg gataccgcgg gaacgccttc atagcgcccg
     1681 gccagcggca ccgtaaagtt ggcaagcggc gcggcggtgg tcgccttggc ttggaggtga
     1741 taaatcggag cgggaatcat ctcccgttcc gccccggcca gcagggcgtg cttgcccatc
     1801 ggcttgatga aatcccatcg catcgggccg gcaatttttc ccgcaaactt cgcgccgaaa
     1861 acctccgcga gcgcataatc ggagcgccgg atgccggtgt cgtcgtacat ggatgtttca
     1921 aaactcgcgg cgagattgcc gccgttgcga acatactcgc gcaggcgggc ggcgacgccg
     1981 ttgctcatgc aagccgcgtt gggcagaaca atggcggaat accggtccag cggttcgctt
     2041 tcgagagtgg tgtcgtcaat tacgtcaaac gggatatgcg cccgggcgag cgccgcggcg
     2101 aacccggaaa attcggtttc gaggttgccg gcgccggaga ccgcaatctg atcgatgtcg
     2161 atcaactggg ctcccgcacc cgcatagaag ttcgcggtgg tttccgacca caccagcgcc
     2221 acgcgcgctt ccgaccgcgt gccccggtag taatcggcgt tgccggctac aaagcggttc
     2281 atcgccgcga tcgcatccat gtccggctgg tcgaattcaa aaggagtgat accgaaccag
     2341 acgctggctc cattggcgat actgtcggcg tacataaggc gcagttcggc cgcaggcagc
     2401 agcgaaaagg tccagggctt gtggctggcg gccgcgaaga tcaccgtcgg ttttccaccc
     2461 gcctgtgtct ccaacagacg cgcggtcagg ccgggtttcc acagcggtac tctcgtgagg
     2521 tcgcccgaaa tgaagccgcc ttcgctgccc agcaggtcct gctcgggaac cagcacgcga
     2581 ttcagtcgtc cggtggccca gttcgatccg cgtacaccgc cgttcatgta gagtgccagg
     2641 tgcgggctga tggatttaag aatggcgttg gagtcacgca aaaaatcggc gaggctcgcc
     2701 gcctggaaat cgacaaggtt cttgaactcc cggccgcggc ggacctgctt ggacggcata
     2761 tcggcgccgt acttcgcacg gaacttctcg cggcaatact tgcagtagca ggtgtccggc
     2821 cggtagaccg gcccgtcgta gaaaatgccg tcgataggat aagcggccag atcgcgcagt
     2881 acctggaaac accatttcct ccagggtgtg ttgacgcaaa aatccgtgcc cgtgtcgtaa
     2941 acgccgtcca gcggtttgcc attttcacga atctgccgcc agtccggatg ctgctggccg
     3001 aatttggttg tataccagtg aacattgaaa tagataaaga cacgaatgcc ctgcttcttc
     3061 gcttcggcga tgtatttcct gagatagtcg ggattcgatt taccggctac ttttgagaca
     3121 aagaagaagc cctggtcgtc caggccgccg ggcatctgca tgatatccag gtgttcggta
     3181 tcgtagccct gctcggcttt ttgtcttgcg aggagagccg gatcgcggta atcgatgcgg
     3241 ctgatgcttg tggtgaggtc gatgattcga agggggccct gctcccacca gggcttttgg
     3301 ggagtggcgc tgcccgccag cataagcgtg gcgagcagcg cgaaaaacaa cttcatcaga
     3361 acagcacctt caatccgaac tggatctgtc tcgggtctcc ggcgccgaag atacggccaa
     3421 cggtggatgg cacggagata ttcgcacccg ggccgccgaa attagcgtga ttcggcatgt
     3481 tgaagaactc cgcccggaac tggatgctgg cgcgttcccg gatggagaag tccttcagca
     3541 ggctcacatc gaagacgata tcacccggtg cgaacagcat attccgggcc gagttgccga
     3601 aggtgaaagg cgcgggcaca gcgaacgccg aggcgtcaaa ccatttgctg atggaccgct
     3661 cggatcgcga gagcctggga tcgcccacct ggtccgcacg gctgctcagc cagcccggtt
     3721 gcgaagcggt aaacgaaacc ccgaagggag gccccgtgcg caaataggta attcctccga
     3781 tctgccaacc tccgactaca tacttcaacg gaccgttgct cttcagaaag ggtttgttgg
     3841 ggccgaacgg cagctcataa gagtaagcga cggtgaagat gtgccggcgg atctggtcgg
     3901 agttgccgcg gtcggcgcgg gcattgtaag gatcttgcgg gccgcctacg acgggaacgt
     3961 tatcgagtga gcgggtccag gaatactcgg cctggaagct gaggccgttg gagtagcgct
     4021 gaatggcctc caactgaagc tggtgcagat tggagtcacc cccgcttgcg agtaacaaaa
     4081 tgccggccca gggctgatag ggccgcctcg cttgaatggc ccctggcgcc tgacgcggct
     4141 cattgaccgg gtagttatac cagggcaggt gcgtggactt gttgccgacg taagaggccc
     4201 gcacaccgag gttactcttc aactcccgct ccaccgtgaa attccactgg taggattcgc
     4261 tgttgcggat gtcccgctgg acggcggttg cactcggatt cggagagagc gcgcccgcgc
     4321 ccgggaacgg agaggcgagc gtgagcgacg gagtccggcc gggatcggat tcgaacgtct
     4381 ccccgagcag gaaaggtgga ttgttgaacc cgagttggcg gaagccgatg tagacgggca
     4441 gggtgttgta gtaaacgcca gccccaccgc gtataacggt cttgttccct ccgaacggcc
     4501 ggaaagcgaa gcctacacgc ggcgcccaat tgttcttgtc ggtctcgaga aggttctcag
     4561 acaggccggc cgcggccgta gtggtgatgg gataggcttc aatcaaccgg ggcagcgctt
     4621 gcgatggaaa ccggtcgccc ggaaccacaa gctggttgga ggcaaaatcg aactgcgcct
     4681 gtgtgcggtc gcgctctttc caaacggtct gcaccatgaa gcgcaccccg aagttcagcg
     4741 tgaaccgcgg cgtcacctgc cagtcatcct gcacgaaggc gctgtaacgc gtcgagtaga
     4801 acaagccgac agggctggtg gtggagcggt acgtgacgtt cggatagccc agcagaaaat
     4861 ccgcgaagcc gtgcgcgggt tgcgccgctc cggtggggtt gttgttcgtg aaccggccat
     4921 tgaaatcgaa ccggccgagc cccgcgttat tggccagccc cgatagcatg cccgcggtgg
     4981 ccgggaaaga tgccgcccgg ttattcgcga tatcgaagcc cgttttgatc gtgtgacggc
     5041 cgcggatgtg cgtgaggttg ttgataaact ggggtgtcag ttgcggcgtg agatcgctcc
     5101 cgccgtaatc tccaattgcg acgtgtccat tgatgttgat gttgggcagc ccgccgtatg
     5161 aaaccggata cagatccggg aacagggaga gcggatcgaa atcgcggttc atgccctgcc
     5221 ggacggacgc atgccggaga tagccgaaac ggaactcgtt cagcgtattt ggtgagaacg
     5281 agtggctgtg ggtgatgttc gcgctttgcg tgctgtagcc gccattctgc caggatccat
     5341 agccttcggg ataagcctgg gcgacaaagt agggatttcc cttcgaatag ttcagattca
     5401 cccagatgga atccttttcc gagaagcgat ggtccacccg aacaccccag cggttgacgt
     5461 cggagatatt cggaagattc actacgtaat tggtcagtgt gccggcgggt ccggctcccg
     5521 cctggttggg cagcgggact ttgtcgatca g
//
LOCUS       DASTMK010000169         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_104569, whole genome shotgun sequence.
ACCESSION   DASTMK010000169 DASTMK010000000
VERSION     DASTMK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104569"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFQ79_07285"
     CDS             <1..322
                     /locus_tag="VFQ79_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139494.1"
                     /translation="PQNPARRGEILQVFATGLGAVTPSGVTGRAGVPGQEVVAPVIVG
                     VNNEGVRVVSAEYAPNMIGIYIVTFEVQANAAGGPDRPFALAAVGSDGNLVFGNGSAI
                     PIEP"
     gene            complement(361..855)
                     /locus_tag="VFQ79_07290"
     CDS             complement(361..855)
                     /locus_tag="VFQ79_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139495.1"
                     /translation="MSVNAMWANRTPALAEVMRAATQGITSGSSEEEFANVLADGLKQ
                     AGVNPAEVDVREVEGSQVSGARQFLVTVKDAVEPAENTPEPLDLTDPVAVLKNALKGA
                     GVDPESLGLTMSDDLIHYPGGAYRSRQISLGDRGGDRYDGDAVLRTPDVAVIEIMNRL
                     GIRA"
     gene            949..1179
                     /locus_tag="VFQ79_07295"
     CDS             949..1179
                     /locus_tag="VFQ79_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU0139496.1"
                     /translation="MSLSERIIVDPEILAGKPVIRGTRLAVEFILELLAAGQSENEIL
                     ANYPGLTHEDILACLAYASYLAREYKAFPIPA"
     gene            1180..1542
                     /locus_tag="VFQ79_07300"
     CDS             1180..1542
                     /locus_tag="VFQ79_07300"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEU0139497.1"
                     /translation="MRLLADENFPRPIVDSLRAEGHDVLWARTDLAGWKDLALLDLAE
                     SETRTILTLDKDFWQIAVQRRRPLEQSGVVLFRVHPATSEQLGPLVRAFAGAGAIWTG
                     HVSIITPNGVEMVAVRRK"
     gene            1607..3127
                     /locus_tag="VFQ79_07305"
     CDS             1607..3127
                     /locus_tag="VFQ79_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU0139498.1"
                     /translation="MALFRTRSAAVYGIDAHLIDVEVDMYPSGTARDFVTVGMPDTAV
                     RESRERIKSALINSGFGYPSKSVTINLAPANVRKEGAGFDLPMAAGILGAMGVIGGAG
                     GYLLVGELSLDGSVRAVRGALSIAICARKADIPNVIVPFENGAEAAVVEGVSVYGVRH
                     LAEVVSLLNQPEQFTARRRETAATPSQDVLAPDFAEVRGQTTSKRALEVAAAGAHNVL
                     MIGPPGSGKTMLAKRIAGVLPPLTFEEALESTKVHSVAGVLPKGAGVLVERPFRAPHH
                     TISDAGMIGGGAGTPRPGEVSLAHHGVLFLDELPEFPRNVLELLRQPLEERSVTVARA
                     NMTLTFPANLMLVAAMNPCPCGFYGDSTRECRCTGGIIQRYLAKISGPLLDRIDLHIE
                     VPAVAYKELRGNGNGTSSAEMRERVERARRTQQARGFNNAQIPSRLLRKLCALDESGE
                     RTLEMAVRRLGLSARAHDRILKVARTIADLEGAENIAAKHLAEAVQYRSLDRNYWT"
     gene            complement(3115..>3742)
                     /locus_tag="VFQ79_07310"
     CDS             complement(3115..>3742)
                     /locus_tag="VFQ79_07310"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0139499.1"
                     /translation="TPGQEVIHPLSNPIKKSGGLVILKGSLAPEGCVIKVTGIERKGH
                     TGPARVFNCEEDAMAAVTRGEIKSGDVIVIRYEGPRGGPGMREMLGVTGAIVGAGLGE
                     SVALLTDGRFSGATRGFMIGHVAPEAAAGGPIAAVHESDTIAIDIENRTIDLEVSQDV
                     LKERLSRWTPPEPRYTTGVFAKYCALVSSASEGAITSPVYVARALQVQ"
BASE COUNT          707 a         1161 c         1143 g          731 t
ORIGIN      
        1 gcctcagaat ccggcgcggc gcggcgagat cctgcaagtg tttgccaccg ggctcggcgc
       61 agtcactccg tccggagtaa ccggccgggc tggcgtgccg ggtcaggaag tcgtcgcacc
      121 cgtgatcgtc ggcgtgaaca acgagggggt tcgtgttgtc tcggcggagt acgcgccaaa
      181 catgatcggc atctacatcg tcacgtttga agtgcaggct aatgcagcgg gagggccgga
      241 tcgtccattc gctttagcgg cggtcggctc agacggcaat ctcgtgttcg gtaacggatc
      301 ggccatcccg atcgaaccgt aggcatgttg cgtaaggcgg tacggtagct ccgtgccgcc
      361 ttacgcgcgt attcccaagc ggttcatgat ttcgatcacg gcgacatcgg gagttcgcaa
      421 cacggcgtct ccgtcgtagc ggtctccacc ccggtcgcca agcgagatct gccggctgcg
      481 ataggcccca ccgggatagt gaatcagatc gtccgacatc gtcaagccaa gtgattccgg
      541 gtccaccccg gcccctttca gagcattttt caacaccgcg acgggatcgg tgagatccag
      601 cggttccggc gtgttctccg cgggctctac agcatctttc acggtgacca aaaattgccg
      661 tgcgccagaa acctgactcc cctctacttc gcgtacgtcg acctccgcag ggttaacccc
      721 agcctgcttc agcccgtccg ccagcacgtt cgcgaactct tcttcacttg aacccgatgt
      781 gattccttga gttgccgcac gcatcacctc ggccagcgcc ggcgtccggt ttgcccacat
      841 tgcatttaca ctcaacaaag acaacctccg cccaattcca ttgcacgtgc gataccgcct
      901 gagagttccg gcttggcccg gtcgaacatg gcacggtaga ctgttggtat gtcgctttcc
      961 gagcgtatca tcgtggaccc ggagattctg gccggcaaac cagtgattcg gggaacgcgt
     1021 ctcgccgtcg agttcattct ggaactcctg gcagccgggc agtctgagaa cgagattctc
     1081 gcaaactacc ctgggctgac acacgaggac attctcgcct gcttggcgta cgcaagctat
     1141 ctggcccgcg aatacaaggc gttcccgatt ccagcgtaaa tgcgcctcct tgccgacgag
     1201 aattttccga ggcccatcgt ggattcgcta cgggccgaag gtcacgatgt gctctgggcc
     1261 cgcacggatc tcgccggatg gaaagacctt gccctgctgg atctggctga atccgaaact
     1321 cgcaccatcc tcacgctcga caaggacttc tggcagattg ccgttcagcg ccgaaggcca
     1381 ttggaacaat ccggcgttgt tctgttccgg gttcatcccg cgacttccga acaactcggg
     1441 ccgctggtac gcgcgtttgc cggcgcaggc gccatctgga ctggacatgt cagcatcatc
     1501 acacccaacg gagttgagat ggtggcagtt cgcagaaaat gaggcggctc atcgggtaat
     1561 tcgttgcgaa aaataccttc agacacgcac tattcctgtc tgtaccatgg cactattccg
     1621 cactcgaagc gcggcagtgt acggcatcga tgcccacctg atcgacgttg aagtcgacat
     1681 gtatccctcc ggaacggcgc gcgactttgt caccgtggga atgccggaca cggcggtacg
     1741 ggagagccgg gagcgtatta aatcggcact gatcaattcg ggattcgggt acccgagcaa
     1801 atccgtaaca atcaatctgg ctccggcgaa cgtgcggaag gaaggcgccg ggttcgatct
     1861 gccgatggcc gcgggaatcc tgggcgctat gggagttatc ggcggcgccg gcggctatct
     1921 cctggtggga gagttgtcgc tggacgggtc cgtgcgcgcg gtcaggggag cgctgtccat
     1981 cgccatctgt gcacgcaaag cggatatccc gaacgtcatc gtccctttcg agaatggagc
     2041 tgaggcggcg gtggtggagg gagtcagcgt ctacggcgtc cggcacctcg ccgaagtcgt
     2101 gtcgctgttg aaccagccag aacagtttac cgcccggcgg cgggaaacag cagcgacacc
     2161 gtcacaagat gtacttgcgc cggactttgc cgaggttcgt ggccagacca catcaaagcg
     2221 cgctctggag gtggctgctg ccggtgcaca caatgtcttg atgattggcc cgccgggctc
     2281 gggaaagacg atgctcgcca agcgcatcgc cggcgtcctg ccgccgctca cgttcgaaga
     2341 agcgttggaa agcacgaagg ttcatagcgt ggcgggtgtt ttgccgaagg gtgcgggcgt
     2401 gctggtggaa cggcccttcc gggcgccgca tcacaccatc tccgatgcgg gcatgatcgg
     2461 cggcggagcg ggaacgccga gaccgggcga ggtcagcctg gcgcatcacg gagtgctctt
     2521 tctggatgag cttccggagt ttccccgcaa cgtcctcgaa ttgttgcggc aaccgctcga
     2581 agaacgttcg gtgaccgtgg cgcgcgcgaa catgaccctc acgtttcccg cgaacctgat
     2641 gctggtggcg gcgatgaatc cctgcccttg cgggttctac ggcgactcca cccgcgaatg
     2701 ccgttgcacg ggtggaatca tccaacgata cctggcgaag atcagcgggc cattgctgga
     2761 ccgcatcgat ctgcacatcg aagttccggc agtcgcctat aaagagctcc gcggtaacgg
     2821 gaacggaact tcgtccgccg aaatgcggga acgggtcgag cgggctcgcc gcacgcaaca
     2881 ggcacgcggt ttcaacaacg cgcagatccc atcgcgcctg ttgcgcaagc tttgcgcact
     2941 ggacgaatcc ggcgagagaa cgctcgaaat ggcggtccgc cgcctgggcc tgtcggcgag
     3001 ggcgcacgac cgcattctga aggtcgcacg caccatagcc gatctggaag gcgccgaaaa
     3061 catcgccgcc aagcacctgg ccgaagccgt acaataccgc agtctcgacc ggaactattg
     3121 gacctgaagc gcgcgtgcca cgtagaccgg ggaagtgatg gcgccttccg aggccgagga
     3181 taccagcgcg caatacttcg cgaaaacgcc ggttgtgtag cgtggctcgg ggggcgtcca
     3241 ccggctgagc cgctccttca gcacgtcctg tgagacttcg agatcgatgg tcctgttctc
     3301 gatgtcaatg gcgatggtgt cgctttcgtg cactgcggcg atgggtcctc ctgccgccgc
     3361 ttcgggcgcc acgtggccga tcatgaatcc gcgcgtagct ccactgaagc gtccatcggt
     3421 gagaagcgcc accgattcgc ccaggcccgc ccctacgatg gctccagtaa caccgagcat
     3481 ctcccgcatt cccgggccgc cgcgcgggcc ttcatagcgg atcacgatta cgtcgccgga
     3541 tttgatttct ccacgcgtca ccgcggccat cgcatcttcc tcacagttaa agacgcgagc
     3601 cggtcccgtg tgccccttgc gctcgattcc ggttaccttg atgacgcaac cttcgggagc
     3661 cagcgagcct ttgaggataa ccaggccgcc gcttttctta atcggattgg aaagtggatg
     3721 gatcacttcc tggcccggtg tt
//
LOCUS       DASTMK010000170         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_200533, whole genome shotgun sequence.
ACCESSION   DASTMK010000170 DASTMK010000000
VERSION     DASTMK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200533"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFQ79_07315"
     CDS             complement(<1..957)
                     /locus_tag="VFQ79_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139500.1"
                     /translation="MYILRHAAIFLAVAPLFAQTVEQPVRSVTDPGMVTTRQAITPAG
                     VPTVFQGRVYGVVFGKSESELWVLHANRIYRLDWLRNTVLNSVPFKGRPGLQGIAFDS
                     HSERAIAAVAPPSGVELHSTDGTTSRLLAGKLGDALAGSLEIASSANADGQRLAAIPI
                     VKDNKLALVDLASQRVLGTAATGIAPFGCVLSRDGRTAYVSNWGGRIPQEKDLTAPTG
                     LAKDADQVVIDKRGIASTGTVTRIDLQSLKTTHTVAVGLHPTAMVWDEESARLYVVNS
                     NSDSISVIDTRKHAVVETISLKPFHTEVKGIAPTAIRLSSDKR"
     gene            complement(1638..1883)
                     /locus_tag="VFQ79_07320"
     CDS             complement(1638..1883)
                     /locus_tag="VFQ79_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU0139501.1"
                     /translation="MRYDSHVQKFRRITMDPEVMGGKPCIRGMRVTVGMIVEALAAGR
                     TIEQLLADFPYLEEADIREALAFAAKLAQGHEVRLAS"
     gene            complement(1915..>2158)
                     /locus_tag="VFQ79_07325"
     CDS             complement(1915..>2158)
                     /locus_tag="VFQ79_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139502.1"
                     /translation="MLPVAVTVGLLLEVLKYLTLILWPWLEQKLKIEYGPFIYSVTIV
                     LWSFFASMLLLAGAEWATRNAGIKPEAASLTQSSST"
BASE COUNT          444 a          631 c          633 g          450 t
ORIGIN      
        1 gcgcttgtcg ctggacaggc ggatcgccgt cggagcaatt cccttcactt ccgtgtggaa
       61 aggtttgagg gagatcgtct cgaccacggc atgcttgcga gtgtcgatga cagagattga
      121 atcggagttc gaattgacta cgtacagcct ggcggactct tcatcccaaa ccatcgctgt
      181 cgggtgcaga cccactgcaa cggtgtgagt ggttttcagg ctctgtaaat cgatccgcgt
      241 aaccgttcct gtagaggcta tgccccgctt gtcgatcacc acctggtcgg catccttagc
      301 gagcccggta ggagccgtca ggtctttttc ttggggaatt cgcccgcccc agttcgagac
      361 gtacgccgtt ctgccatccc ggctgagcac gcacccaaac ggggcgatgc ctgtcgcggc
      421 cgtgcctaga acgcgttggg acgccagatc gacgagtgcc agcttgttgt cttttactat
      481 ggggattgcc gccagacgct ggccgtccgc attcgccgaa gaggcgattt cgagcgaacc
      541 ggcgagcgcg tctcccagct tgccggcaag gagccgggac gtagtgccat cggttgaatg
      601 cagttccacc ccggacggcg gtgccaccgc ggcgatcgct ctttccgaat ggctgtcgaa
      661 ggcgatgccc tgcaaacccg ggcgtccctt gaacggcact gaattcagca ccgtatttcg
      721 aagccaatcc aaacggtaga tgcggttggc gtgaaggacc caaagctcgg attccgattt
      781 gccgaaaacc actccgtaaa cgcgtccctg aaacaccgtc ggaacacctg ccggcgtaat
      841 agcctggcgg gtggtcacca taccgggatc ggttacggaa cgtacgggct gttcaaccgt
      901 ctgcgcaaag agcggcgcaa cggccagaaa aatagcggcg tggcggagga tgtacacgcc
      961 gcatgatatc aattttttag ttaatgcttg atttcgagag ggaccggcga ccgccggtta
     1021 tcatcgcgct caagtgaaac ggcctcctcc tccggatggt ccgcagccgc cggtagcaaa
     1081 agacgatagc tgccgttcga cctggcggga gccgcactcc gaacagttca aatcgcggtc
     1141 ggcatcctgc atccgccgca gttgttcaaa catttttccg cagtcctggc agcggtactc
     1201 gtacattggc atggctgttc cacgatacag gatgcaggat cagcgatagg gtgacagagt
     1261 tgtgggaacc taatgcagcg gtagcactcg ggcgcgcggt tggtccgacc agtcgagcga
     1321 tatgacagca ccctgggcta tttctctttg acatttgaca agagcctcaa ggagcgcccg
     1381 gccccgggac tcgggtacca gtggctcgcc acgcaggagg acgacgctcg gtcctgtatc
     1441 acgcgtgtga gcaagaatct gtggaaaatc aagatcgttc gtcaggagca cgagtctgtt
     1501 tgtgcgggcg tactcgcaga tttcgcgatc ttttgccgac gcagcgccta tcaatgacca
     1561 atggtacgtt tcatgtcccg caccgttgag atattcaacc caacgcggcg tcaggttcat
     1621 gtcgaccaga atgcgcatca gctcgcgagg cgaacttcat gtccttgcgc cagcttggcg
     1681 gcgaaggcaa gcgcctcccg gatgtccgct tcttccaggt aggggaaatc ggcgagcaac
     1741 tgctcgatcg tgcgtcccgc agcaagcgct tccacgatca tgccgaccgt tacacgcatg
     1801 cctcgaatgc acggttttcc gcccatgacc tccggatcca ttgttatccg ccggaacttc
     1861 tgcacgtggc tatcataacg catcgggtga aaccgggccg cgccggggcg tgtctcacgt
     1921 tgaggaactc tgtgtcaaag aagcagcttc gggcttgatt cctgcattgc gtgtggccca
     1981 ttccgcgccg gccaggagca gcatgctggc gaaaaagctc cacaatacga tggtgaccga
     2041 gtagataaac gggccgtact cgatttttag tttctgttct agccacggcc ataggatcag
     2101 cgtcaggtac ttcagcactt cgagaagaag acctaccgtc accgcgaccg gcaacatg
//
LOCUS       DASTMK010000171         7100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_456264, whole genome shotgun sequence.
ACCESSION   DASTMK010000171 DASTMK010000000
VERSION     DASTMK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456264"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /locus_tag="VFQ79_07330"
     CDS             <1..1642
                     /locus_tag="VFQ79_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139503.1"
                     /translation="WRLAEAAQESDFILLSELLAKGLKQMPENTVFRQMASGACRGQN
                     QPRMAERIPAMFPCEQVNPVPWSVDLPPMPSGAPAWAVAQRMLATRLEKLTAWWIEER
                     QQENGELGGGWGDDVEILRSWGPQALGFGSQVAAQGLRKLAEGLWRNGNIVNGYAKEI
                     SDSEHSAEDIADTQPLLAATFPEDPEIIARLKQTTACSENWLIRQPDGFWRFRGSWFN
                     CSEFSPNPDHAIDVHYNVRAMGPALWYASLTRDPTVIERVVRWAESWVRALRSTDHGK
                     PAGVIPPVLISSNGSYLVRSQHWDKPNAFWDYFHWSGYSQEGLTNLLLAVYDLTGEKK
                     WLDAVGETFQVLEHCRTYPRICSEIRQRPEAFYEWRRLTGSTRYDAQFPNLPDTDATR
                     ALALMEAEAQKAVERIGYNFDLYTSEPMWTDRIPYGLLSAEYKSYLFGGDAPRGERYP
                     TFAVTWPATKASFARAVLNTTGTSAELRLYSFEPQTVHIPVRLWRLQRGKYEWESSPV
                     GRGPSMRREFEFNRSAQKIELPLPPREEVTIKIKRISD"
     gene            complement(1652..2320)
                     /locus_tag="VFQ79_07335"
     CDS             complement(1652..2320)
                     /locus_tag="VFQ79_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0139504.1"
                     /translation="MSEKKPLNPRNPTQTERAYAALKDAILQGEIHEGVFLSEREIMR
                     RYRIGRTPFREACNRLHHEGLLEVVPRRGYLVPELSFHAVRDLFELRLILEGAIAELA
                     TIRGTDSEIDELEDSTRHAALKLKKGVDSLEFVRLNSDFHLKLARMTRNRELERLLVE
                     VLEKTQRLMYIELRWSRLPQGQGKMLHEPIVAALRRRTPGAVREALLEDIRQAQSETL
                     GKGL"
     gene            2388..3242
                     /locus_tag="VFQ79_07340"
     CDS             2388..3242
                     /locus_tag="VFQ79_07340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0139505.1"
                     /translation="MSILSRRELIATPLVLAQGAEPKPSIWDVHCHLTSAEGSTPEER
                     MAYLIRFMDRLGIERVMLSLGYPLLADPSPEQLREANDQVLKAVARWPDRALGFVYLN
                     PNQLAFSLGEFDRCVRDGPMVGVKLWVAKRCNAPELDSIVERAAAMQAPILQHAWFNL
                     KGNLPGESTPNDLAELAMRHPKAVFIDAHTGGDWERGILALRPAKNVATCVAGFDPTA
                     GIVEMAVRELGAERVMFGSDAAGRSFASQLGKVMGAEIPERSRRLILGENLRRILTPI
                     LREKGRPV"
     gene            3239..4009
                     /locus_tag="VFQ79_07345"
     CDS             3239..4009
                     /locus_tag="VFQ79_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0139506.1"
                     /translation="MIVDVNVNLSRWPFRRLPGDEPQVLAAKLRKLGVTQAWAGSFDG
                     VFHKDIAGVNARLAADCREFGAGLLLPFGSVNPKLPDWREDLRRCVEEHKMPGIRLHP
                     NYHGYTLDDPEFQELLKLAAARKLVVQLALCMEDERTQHPLMRVPPVDIGPLANAVAR
                     ERQLKLVILNSYPQLPLERLRPLSSAGQVYFDLSMVERVGGVTRLSEYVSPGRVLFGS
                     NYPFFVFESALLKMQEAELAETQRAAIFEGHARRLLAR"
     gene            4018..5988
                     /locus_tag="VFQ79_07350"
     CDS             4018..5988
                     /locus_tag="VFQ79_07350"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0139507.1"
                     /translation="MTRLILLCTTTLSAAFAQDPLLAWMNRIAQEQLAKRDAAVAKIQ
                     TPSDAEQRRQWIRSTLLDILGGLPEYRGPLNARVLGRLENPHYTIEKVIFESLPKYYV
                     TANLYRPNQPGRYPAVLMSAGHTTLGKTENHIMSAHLAAKGFVALAYDPVGLGERVQS
                     YDRNAHKRIAGCCADEHLHAGAQSLLIGESVARYFIHDAMRGIDYLVSRPDVDPERIG
                     AAGCSGGGCVTTYVAALDPRVKAAAPACFLNSLRVLFAGRFPDSEMSLPRFLASGLDH
                     ADFLAVSNGIPWLILATEEDFFTPAGVLPVYEEARRWYRLFGAEDKMQMFMGPGKHGT
                     PLETREKLYEFMIRWLQPGSGVDYREGNITLYPDQELQVTQAGQVEFEEGSRWLYQVI
                     RDRFQLRKRPRGVPQLLAELRRLGIPSEGQPPAFTTMERRQGRTRLTLESEPGIEIGA
                     TLYLPGAEGRKPALLLVKDAATAQLAETAAARGNVVLELEPRDSPAANDNRPFLGNWL
                     TNKRADSIGRNLPAMRAYDILRGVDHLHARNDVDRSRIRAAARNVEGVWLLLAAAADP
                     RIAKIWLDRTPHSLSAAMDGPIHTRLFDSVIPGFLLYWDFEDLVRAMGKRQVMWTDPP
                     DWMLRTRRGLGSGFRYREPGQTEEALLAEFLQ"
     gene            complement(5998..>7100)
                     /locus_tag="VFQ79_07355"
     CDS             complement(5998..>7100)
                     /locus_tag="VFQ79_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0139508.1"
                     /translation="ERPTHHSLRPAPPVLKPQVKSSGKFRVGITPDFETEANGALSPA
                     LREVLEPVAGVQYEIMPDTGGIGDPAVLDRYDGVIAYSYGFPASSFRGIGRLSVLALW
                     GVGFDRVDVAACTEAGVILALTRDSVARPVAEGIFALIFSLAKNLRALDIQCRFGRWR
                     QNPPTLMDLAARTLGCIGFGGIAREMFRMAGSLGFERLLAYAPRATAAEADAAGVQLA
                     SLETVLRDSDFVTINCPLTDETRHMIGARELGLMKSTAYLINTARGAVVDESALADAL
                     RWKKIAGAGLDVFETEPAAAGNPLFELDNVIVTPHLIARTEACIRNTSLSACRNVLAV
                     ASGTPPPYVANPEVLQQPRVRARLEDRRFQRK"
BASE COUNT         1377 a         2071 c         2222 g         1430 t
ORIGIN      
        1 ctggcggctg gccgaggccg cccaggaaag cgacttcatt cttctgtccg aactgttggc
       61 caaaggtctg aaacaaatgc cggagaacac ggttttccgg caaatggcct ctggagcttg
      121 ccgcggtcag aatcagccgc gcatggcgga acggattccg gcgatgttcc cctgcgagca
      181 ggtgaaccct gttccctggt cggtagattt accgcctatg ccttccggcg ctcctgcctg
      241 ggcggttgca caacgaatgc tcgctacgcg tctggagaag ttgaccgcct ggtggattga
      301 agaacgtcag caagagaacg gagaactcgg cggaggctgg ggcgacgacg tcgagatcct
      361 gcgaagttgg ggtccacaag cattgggctt tggctcgcaa gtcgccgcgc aagggctgcg
      421 gaaactggcg gaaggcttgt ggcgcaacgg gaacatcgtc aatggctatg cgaaagagat
      481 ctccgattcc gaacactcgg cggaggatat cgcggatacg cagcctctat tggcggccac
      541 gtttccggag gatccggaaa ttatcgcacg cctgaaacag acaaccgcgt gttccgaaaa
      601 ctggctgatt cgtcagccgg acggtttttg gcgtttccgc ggttcctggt tcaactgttc
      661 tgaattcagc ccgaatccgg atcatgcaat cgacgttcac tacaacgtcc gtgcgatggg
      721 cccggctctc tggtatgctt ctttgacccg cgatccgacg gtcattgaac gggtggtgcg
      781 gtgggcggag agttgggttc gcgcacttcg ctcgacggac cacggcaagc cggcgggagt
      841 gattcctccc gtgctgattt cctccaacgg ctcttatctg gtccggtcgc aacactggga
      901 taagcccaac gcgttctggg actatttcca ctggtcggga tattcgcaag aaggtctcac
      961 caaccttctg ttggccgtgt acgatcttac cggcgagaag aaatggctgg atgcggtagg
     1021 cgagactttc caggtcttgg agcactgcag gacctatccg cgaatctgct cagagatccg
     1081 gcagcggccg gaggctttct atgagtggcg gcgtttgaca ggttcgactc gctacgacgc
     1141 tcagttcccc aacctgccgg acaccgatgc gacgcgagcc ctggcgctga tggaagccga
     1201 ggcacaaaaa gccgtggaac gcatcggcta caacttcgac ctatacacat ccgagcccat
     1261 gtggacggat cgcattccgt acggcctgct atcggccgaa tacaagtcgt acctctttgg
     1321 aggcgatgcg cctcgcggtg aacgctaccc gacatttgcc gttacctggc ctgctacaaa
     1381 ggcgagtttc gcgagagcag ttctgaatac gaccgggacc agtgcggaat tgaggctcta
     1441 cagcttcgaa ccgcagactg tgcatatacc ggtgcggctc tggcgccttc aacgcggcaa
     1501 gtacgaatgg gaaagcagtc ccgtcgggcg agggccgtcc atgcggcgtg aattcgaatt
     1561 caaccggagt gcacaaaaga ttgaacttcc gttgccaccg cgggaggaag tgacgataaa
     1621 gatcaagagg atatcggact gaaccggacg ctcagagtcc tttcccgagc gtctccgact
     1681 gggcttgccg gatatcttcg agcagggctt cccgcacggc accgggggtc ctccgccgca
     1741 atgctgccac gatgggctcg tggagcattt tgccttggcc ttgcggcaga cggctccaac
     1801 gcaattcgat gtacatcaga cgctgtgttt tctcgagtac ctcgacgagc agtctctcca
     1861 actcgcggtt gcgagtcatt ctggcgagtt tcagatggaa atccgaattg aggcgaacga
     1921 actccaggga gtccactcct tttttgagct tgagcgccgc atgcctggtc gagtcctcca
     1981 gctcgtcaat ttcggagtcc gttccacgaa tcgtagcgag ttcggcaatt gcgccctcca
     2041 ggatgagccg tagctcaaac aggtcacgca ccgcatggaa gcttaactcc ggcaccaggt
     2101 agccgcggcg cggcactact tccaggagcc cttcgtgatg aagcctgttg caagcctcgc
     2161 gaaatggggt ccggccgatt cggtaacggc gcatgatttc gcgctcggac agaaaaacgc
     2221 cttcatggat ctcaccttgt aggatggcgt ctttcagcgc cgcataagct ctttcggtct
     2281 gagtaggatt tcgtggattc agcggctttt tctcgctcac gccggatagt gtagcctaaa
     2341 ccgggacggt atagtaggct cggcttagcc gacgtagaat atattctgtg tctatattaa
     2401 gtcggaggga gttgatcgcg acgcccctgg tgcttgccca aggcgccgaa ccgaaaccgt
     2461 ccatctggga tgtgcattgc cacctcactt cggcggaagg atctacgccg gaagaacgga
     2521 tggcttatct catccggttt atggaccgcc tgggaatcga gcgtgtaatg ctctctctcg
     2581 gctatccttt gcttgccgat ccatctccag agcagcttcg ggaggcgaac gatcaggtgc
     2641 tgaaggcggt cgcccgctgg ccggaccgcg cgctgggctt tgtgtatctc aatccgaatc
     2701 agctcgcgtt cagcctgggg gagttcgacc ggtgcgtgcg ggacggcccc atggtggggg
     2761 tgaagctgtg ggttgcgaaa cgctgcaacg cgcccgagct cgactccatt gtcgagcgcg
     2821 ccgcggccat gcaggcgccc atactgcaac acgcgtggtt caacctgaag ggcaacttgc
     2881 cgggtgaatc gacgccgaac gatctcgccg aattagccat gcggcatccg aaggctgtgt
     2941 ttatcgatgc gcacaccggc ggcgattggg aacgtgggat tctcgctctc cggccggcca
     3001 agaatgtcgc gacgtgtgtc gccggtttcg atccgacagc cggcatcgta gaaatggcgg
     3061 tccgcgaact cggagcggag cgcgtgatgt tcggaagtga tgccgccggg cggagtttcg
     3121 cctcgcaact cggcaaggtg atgggagctg agattccgga acggtcccgc cgcctcattt
     3181 tgggagaaaa cctgcgccgc atactaactc cgatcctgcg ggagaagggg cggccagtat
     3241 gattgtcgac gtcaatgtga atctgtcgcg ctggcctttt cgccgcctgc cgggcgacga
     3301 gccgcaggtg cttgccgcca agctacggaa gctgggcgtt acgcaggctt gggccggcag
     3361 cttcgatggc gtattccaca aggacattgc gggcgtcaat gcccggctcg ctgccgattg
     3421 tcgcgaattc ggcgccggac tgctgctgcc cttcggctcg gtgaacccca agctgccgga
     3481 ctggcgggaa gacctgcgcc gctgtgtgga agagcacaag atgcccggca tccggctgca
     3541 tcccaactat cacggatata cgctcgacga cccggagttt caggagttgt tgaagctggc
     3601 tgccgctcgc aagctggtgg tgcaactcgc tctgtgcatg gaagacgagc gcacgcagca
     3661 tccgttgatg agagtgccgc cggtagacat tgggcctttg gcgaacgccg tcgcccgcga
     3721 gcgtcagttg aagctggtga tcttgaactc ttatccacaa ctgccgctcg agagattacg
     3781 gccgctgagt tccgcgggcc aggtctattt cgatctctcc atggtggagc gtgtgggcgg
     3841 cgtgacgcgg ctcagcgagt acgtctcgcc cggtcgcgtc ctcttcggct cgaactaccc
     3901 ttttttcgtt ttcgagtcgg cgttgctgaa gatgcaggaa gcagagctgg cggaaactca
     3961 acgggcggcg atcttcgagg gccatgcacg ccggttgctg gcaaggtaac gatgcgattg
     4021 acgagactca tactattgtg cacgaccacg ctctccgctg cgttcgccca ggaccccctg
     4081 ctcgcatgga tgaatcgcat cgcacaggag cagcttgcga aacgggatgc tgctgtcgcg
     4141 aagatccaga cgccgtctga cgccgaacag cggcggcaat ggattcgctc gacgctgctc
     4201 gacatcctcg gcggcttgcc ggaatatcgc ggaccgttga acgcgcgtgt gcttggccgt
     4261 cttgaaaacc cgcactacac aatcgaaaaa gtcatcttcg agagccttcc gaaatattac
     4321 gtgacggcca acttgtaccg gccgaatcag ccaggccgct atccggcagt gctcatgtcc
     4381 gctgggcata caacgcttgg aaaaaccgag aaccacatca tgtcggcgca tctggcggcg
     4441 aaaggctttg tggctctggc ctatgatccg gtgggtttgg gagagcgcgt acagtcctat
     4501 gaccggaatg cgcacaagag aatcgcgggc tgctgcgcgg acgaacacct tcacgccggg
     4561 gcgcagagcc tgcttatcgg tgagagtgtc gcccggtatt ttattcatga cgcgatgcga
     4621 ggtatcgact atctggtgag ccgccccgat gtagaccccg agcgtattgg agctgccggc
     4681 tgctcgggcg gcgggtgcgt caccacctac gtagcggcgc tcgaccctcg cgtcaaggca
     4741 gccgctccgg cgtgtttcct caacagtctc cgtgtgctct tcgcaggccg gttcccggat
     4801 tcggaaatga gcctgccgag atttcttgca tccgggctgg accacgcgga tttcctcgcc
     4861 gtatcgaacg gaataccctg gctcattctg gcgacggagg aagacttctt tacccctgcc
     4921 ggtgttctgc cggtttacga agaggcgcgg cggtggtatc gcctcttcgg cgccgaagac
     4981 aagatgcaga tgttcatggg tccggggaaa catggtacgc ctctcgaaac gcgcgaaaag
     5041 ttgtatgagt ttatgattcg ctggctgcaa ccgggaagcg gtgttgatta tcgcgaaggg
     5101 aatatcacgt tgtacccgga ccaggagttg caggtaaccc aagccggcca ggtggagttc
     5161 gaggagggaa gccgttggct ctaccaggtg attcgagacc gctttcagtt acgcaaacgt
     5221 ccacgcggcg ttccgcagct tctggctgaa cttcgacggc tcggcattcc ttccgaaggc
     5281 cagccgccgg cgttcaccac gatggaacgg aggcagggcc gcacacgcct gacgttggag
     5341 agcgagcctg gtatcgagat cggagcaacg ctttatctcc ccggcgccga agggcgcaaa
     5401 ccggcgctgc tgctggtgaa ggacgctgcg accgcgcagc tcgccgaaac agcggctgca
     5461 aggggaaatg tggtgttgga actggagcct cgcgactctc cggccgcaaa cgacaaccgc
     5521 ccgtttctgg ggaactggct gaccaacaaa cgcgcggatt caattggccg caatctgccg
     5581 gcgatgaggg cctacgacat tctgcgtggc gtggaccacc tgcacgcacg taacgatgtg
     5641 gatcgctcac gaattcgtgc ggcggcacgc aatgtcgagg gtgtctggct gctgctcgca
     5701 gcggctgcgg acccacgcat tgcgaagatc tggctcgacc gcacacctca cagtttaagc
     5761 gccgcaatgg acggcccgat ccatacacgg ctgttcgata gcgtcattcc ggggttcctg
     5821 ctgtactggg acttcgagga cctggtgcgc gcgatgggta agcggcaagt gatgtggacc
     5881 gatccgccgg actggatgct gcgcacgcgt cgtggcttgg gctcgggatt ccgctatcgc
     5941 gagccggggc aaacagaaga agcgctgctg gcggagtttt tacagtagag cctgatccta
     6001 tttccgttgg aagcgccggt cctccagccg ggcacgtact cggggttgtt gcagaacctc
     6061 cggattggcg acatagggcg gcggcgttcc ggatgcgacc gccagtacat tgcggcaggc
     6121 ggagaggctc gtattacgga tgcacgcctc ggttcttgcg atcaaatgcg gcgtgacgat
     6181 tacgttatcg agctcgaata gcggattgcc ggctgccgcc ggttcggttt cgaagacatc
     6241 gagacccgcg cccgcaatct tcttccaccg cagagcatcg gctagcgccg attcgtccac
     6301 caccgctcct cgggcagtgt tgatcagata tgccgtcgat ttcataaggc cgagttctcg
     6361 tgcgccaatc atgtggcggg tctcgtctgt cagcgggcaa ttgatggtga cgaaatcgct
     6421 gtcgcgcagg acagtctcga ggctggcgag ttgtactccg gcagcgtccg cctcggcagc
     6481 cgtcgctcgg ggagcataag cgagcagccg ctcaaacccc aggctgcccg ccatgcgaaa
     6541 catctcgcgc gcgatgccgc cgaatccgat gcacccgagc gttcgtgcgg cgagatccat
     6601 cagcgtcgga gggttctgcc gccaacggcc gaagcggcac tggatatcga gcgcgcgcag
     6661 attcttggca agcgaaaaga tcagcgcaaa aatcccttcc gctacaggac gggcgaccga
     6721 atcacgcgtc agggccagaa tgacgccggc ttcggtgcat gcggcgacgt cgacgcgatc
     6781 gaagccgacg ccccaaagcg ccagcaccga cagacgcccg atgccgcgaa aactgctggc
     6841 tggaaagccg tagctatatg cgatgacgcc atcgtagcgg tcgagcaccg ccggatcgcc
     6901 gatgccgccg gtgtccggca tgatctcgta ctgaacaccg gcgacgggct ccagcacctc
     6961 ccttaacgcg ggagagagtg ctccgtttgc ctcggtctcg aagtctggcg taatacctac
     7021 gcgaaatttg ccgctgctct tcacctgcgg tttcagaact ggaggcgcag gccgaagcga
     7081 atggtgcgtt ggtcgttctc
//
LOCUS       DASTMK010000172         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_297080, whole genome shotgun sequence.
ACCESSION   DASTMK010000172 DASTMK010000000
VERSION     DASTMK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297080"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="VFQ79_07360"
     CDS             <1..1529
                     /locus_tag="VFQ79_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139509.1"
                     /translation="AVRFVFGYTRNAQDGPALSTVQQFGPLQDEFVLFSRVRRLRNEY
                     RVGGEIRVFGATLLWLRGWDNFKEDSTYDLDQPAGNNAVDPVVLSRFGRVEPYHGNTP
                     YWRFALLKEASGWLGVNMRYTYSEGRRNFVLDELASGMAFGAERNRAILVFGTGRRPV
                     SAGSITVSVFPRERVSLTNHTAFHNTRMEGDNTYREFSNSQALLRSLNFSLLAIRTIT
                     NSTDLNVRASRWLGLFTGYRFSSRKIESIQQSDFGFGVESLAAKQTNNLHAGAAGIRL
                     LPAKGVRVNLEGEIGRANRPFFPISDRNYHTASGRVQYRAGQISLSASAKLFYNTNSV
                     SLSAHSAQSRTYSFDAAWTPATWFSVDGGYSKAHLDTTSGLAYFADSTLIEGTSSIYI
                     SNIHSANAGLRLSLGRRADFYAGYVKVQDTGDGRAGLLQPAPAAQALQQTGPAFDTFY
                     PAQTFPLTYHSPMARISFRIHSKLRWNAGYQFYDYGEKFQFPQNYRAHTGYTSLLWSF
                     "
     gene            complement(1539..1877)
                     /locus_tag="VFQ79_07365"
     CDS             complement(1539..1877)
                     /locus_tag="VFQ79_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0139510.1"
                     /translation="MKDAKSRVKEINIEEYRALKEAGTPHVLVDTREDSEWAAGHATD
                     AVHLGKGVIERDIETKIPDKNTKLILYCGGGFRSALAADALQKMGYTDAISLDGGWRA
                     WQTVGLPVTK"
     gene            complement(1913..>3161)
                     /gene="murA"
                     /locus_tag="VFQ79_07370"
     CDS             complement(1913..>3161)
                     /gene="murA"
                     /locus_tag="VFQ79_07370"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU0139511.1"
                     /translation="FSVRGGIPLKGEIAVSGSKNSGLPALAACLLTDQPVVLHRVPRV
                     RDIRTMKSLLASTGAKVDRRGSGPLSVHAERIVRPEAPYDLVKTMRASSLVLGPLVAR
                     TGRARVSLPGGCAIGARPINLHMRALEQLGARIRQEHGYVEAEAPEGLHGAAIHFDRI
                     TVTGTEDLMMAAVLAKGETILENAAREPEVSDLAELLAKMGAQIQGAGTSTIRIQGVD
                     HLGGAEHTIIPDRIEAGTFLIAGAITGGDITVTGAIAEHLTALLLKLGEAGVEVNTDG
                     GLIHVQSSGSLRAVDATTEEYPGFPTDLQAQFMALMTQAEGISFITETIFENRFMHVP
                     ELVRMGANIRIEGRQAIVAGKRDLSGAGVIASDLRASASLVLAGLVAKGETVVDRVYH
                     IDRGYEKIEGKLMRAGAQIRRFD"
BASE COUNT          608 a          980 c          895 g          678 t
ORIGIN      
        1 ccgccgtaag gttcgttttc ggatataccc ggaatgcaca ggacggcccc gcgttatcta
       61 ccgtgcagca gttcgggcct cttcaggatg agtttgtgct gttctcgcgc gtgcggcggt
      121 tgaggaacga ataccgggtc ggcggtgaga tacgagtatt cggcgccacg ctgctgtggc
      181 tccgaggttg ggacaacttc aaggaggata gcacgtacga tctcgatcaa cccgccggca
      241 acaatgctgt cgaccccgtt gttctctcga ggtttggacg cgtggagccg taccacggca
      301 acaccccgta ttggcgcttt gcgctgctca aggaagcgag cggttggctc ggagtgaaca
      361 tgcgctacac ttactcggaa gggcgccgca acttcgtttt ggatgaactg gcctccggaa
      421 tggcgtttgg cgcggagcgc aaccgcgcga ttctcgtgtt cgggacaggg cgccgtcccg
      481 tcagcgccgg cagcatcacc gtaagcgtct tccctcgtga aagagtcagc cttaccaatc
      541 acactgcctt tcacaacaca aggatggaag gcgacaacac ttaccgggaa ttttcaaatt
      601 cgcaggcgct gttgcggtcc ctgaacttca gcctgcttgc tatccggaca attacgaact
      661 ccacggatct caatgtccgt gcgtcacggt ggttgggttt gtttacgggt taccgctttt
      721 ccagcaggaa gatcgagtcc atccagcaat cggatttcgg tttcggcgtg gagagcctcg
      781 cggcgaagca gaccaacaac ctgcatgcgg gcgcggctgg aatccggctt ttacccgcca
      841 aaggcgtgcg cgtgaatctc gaaggcgaaa tcggacgcgc aaacaggccc ttctttccta
      901 tttcggatcg aaactaccac accgcttcgg ggcgggtgca ataccgggct gggcagattt
      961 cgctttccgc gtcggcaaag ctcttctaca acacaaattc ggtctctctt tccgcgcaca
     1021 gcgcacagtc gcggacttat tctttcgatg cggcctggac tccggctacg tggttttctg
     1081 tagacggcgg ctattcgaag gcgcatctgg acactacttc cggactggct tatttcgcgg
     1141 actccaccct gatcgaagga accagttcca tctacatcag caacatccat tccgcgaatg
     1201 cgggcctgcg gttgagcctc ggacgccgcg ccgattttta tgcgggatac gtaaaagtgc
     1261 aagacaccgg agatgggcgt gcgggcctcc tgcaaccggc gccggcagcg caggcgctcc
     1321 agcagaccgg cccggccttc gacacattct atccggcgca gacgttcccg ctcacctacc
     1381 actcacctat ggcgcgtatc tcattccgca tccatagcaa gctgcgctgg aatgccggct
     1441 accagttcta tgactatggt gagaaattcc agttcccgca gaactacagg gcgcacaccg
     1501 gctacacgag cctgctgtgg tcgttctgag gccacctgct acttggtgac cggaagaccc
     1561 accgtctgcc atgcccgcca accaccatcg agcgaaatgg catccgtgta ccccatcttt
     1621 tgaagcgcgt ccgcggccag cgcggagcgg aacccgccgc cgcaatacaa aatcaatttg
     1681 gtgttcttgt ctggaatctt tgtttcaata tcgcgctcga tcacgccttt gcccaagtgg
     1741 acggcgtccg ttgcgtgccc ggccgcccac tcgctgtctt cacgagtgtc gacaaggacg
     1801 tggggagtgc ctgcttcctt cagagcccgg tactcctcaa tattgatctc tttcacgcgc
     1861 gacttcgcgt ccttcactag agacagaaaa ccagggttat gtggcatagt cttcaatcga
     1921 aacgccggat ctgtgcgcca gcgcgcatca atttaccttc aattttctcg tatccgcgat
     1981 caatgtgata cacgcggtcc acgacggttt cgcctttggc aacgagtccg gcgagcacca
     2041 gcgaggcgct ggcgcgaaga tcggacgcaa tgacacccgc gccgctcaaa tcccgcttgc
     2101 cggccacgat ggcctgccgc ccctctatac ggatattggc acccatccgg accagttccg
     2161 gcacgtgcat gaagcgattt tcgaagatcg tttccgtgat gaacgaaatc ccctcggcct
     2221 gcgtcatcag cgccatgaat tgcgcctgga gatcggtcgg aaaaccaggg tattcttcgg
     2281 tagtggcgtc caccgctcgg aggctgccgg aggattgcac atggatcagg ccgccgtcgg
     2341 tgtttacttc cactccggct tctcccaatt tcaacagcag cgctgtcagg tgctcggcga
     2401 tagcacccgt caccgtgata tcgccgcccg taatcgcacc cgctatcaga aaggttcctg
     2461 cttcaatgcg gtccgggatg atggtgtgct cagcgccccc aaggtggtcg acaccttgaa
     2521 tacgaatggt ggaggtgcct gccccctgta tttgcgcgcc catcttggcg agcagttctg
     2581 ccaggtcgga gacttccggt tcccgggcgg cattctcaag aatcgtttcg cctttggcca
     2641 aaaccgcagc catcatcagg tcttcggtgc cggtaaccgt aatacggtcg aaatgaatgg
     2701 ctgctccgtg aagcccttcc ggcgcttccg cttccacata gccgtgttcc tgccggattc
     2761 gtgctcccag ttgttcgaga gccctcatat gcagattgat gggccgcgcg ccgatggcgc
     2821 agcctccggg aagcgaaacc ctggccctgc cggtgcgtgc caccaggggc ccgagcacca
     2881 ggctggaggc gcgcatcgtc ttcacgagat cgtagggcgc ctcggggcgg acgatccttt
     2941 ccgcatgcac cgacagcggg ccgcttcctc tccgatcgac cttcgcgccg gtggaagcaa
     3001 gcaggctttt catggttcgg atatcgcgca ctctcggcac acggtgcaga actaccggtt
     3061 gatcggtgag cagacaggcc gcgagagcgg gtaaaccgga atttttcgat ccgctgaccg
     3121 cgatttcgcc tttcagcggg atcccgccgc gtacagagaa c
//
LOCUS       DASTMK010000173        19436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_360762, whole genome shotgun sequence.
ACCESSION   DASTMK010000173 DASTMK010000000
VERSION     DASTMK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19436
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360762"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFQ79_07375"
     CDS             <1..559
                     /locus_tag="VFQ79_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139512.1"
                     /translation="FAPGTGAGDDTIQFSTGGRTVDFSIPAGSTAAVFSSPQVALQTG
                     TVAGTITVAARLASAGVEITPSSPPTHAIRIEQSRPMLTSARLTRTATGFSIEITGYS
                     TAREVTQAVFRFGAAPGQRLEQPEITMDVRNLFTAWFEDAASAPFGSQFTFTQTFTVS
                     GDAAAVTPQTVVLRNSLGESSITVQ"
     gene            complement(637..1062)
                     /locus_tag="VFQ79_07380"
     CDS             complement(637..1062)
                     /locus_tag="VFQ79_07380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0139513.1"
                     /translation="MSFSLECCSAYGAVLMTSDDEERQGPLTERRRRTRLPLQLPVYL
                     CRRSETHPMESRTVNLSSEGFYCIVQEPLTPGEHIDCDILFPSHNKEKKDTFLCLRCQ
                     ALVVRVETIGRSARFGLACRIEHYRVIQREETPITSSRV"
     gene            1479..2732
                     /locus_tag="VFQ79_07385"
     CDS             1479..2732
                     /locus_tag="VFQ79_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0139514.1"
                     /translation="MNVLLVSNDAELQKLIRAVLAECLGAAWRLVIATQQDLTRKADV
                     YIWDYHSGLRMSDFIDPSEHQKHFVLVQRKQLSQFREESLPDVNVVLKPVSAAALKAF
                     LGEACARWQRQQAEHEDEAGSVRADRDEVLQSLIEANLKLQEYDQDRTNFLARAIHDF
                     RAPLTALTGYCGLLLGEQLGALTADQREVIQRMQFSAKRLSRLASEMFQLSICHRVVT
                     RPNLRRHDIHECVDQALHEIAPFAETKRISVSVDLQQPAEPLAFEPSELEQVLVNLLD
                     NACKFTPRSGSIDVKGYPFFWERRKDGITEMPLRADRRTSNGHAPNSFRIDISDSGPG
                     IPPNHIETIFEEYTSYAGGQDRSGGGLGLAICKMIMNRHHGRIWAESSPRGAVFSLVL
                     PSPAAEHLLATDAAPSENKYYTQVS"
     gene            2736..4223
                     /locus_tag="VFQ79_07390"
     CDS             2736..4223
                     /locus_tag="VFQ79_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0139515.1"
                     /translation="MQAIKNGRTILVGEDEPEVRSYLEMALRCQGYTVELAENGDEVL
                     ACLQDSGEHISAVLLDIVMPRKDGLETLKEIRRIRDDLPIIMVSGASSPLNVVEAMKN
                     GATDFIGKPVNHEDLRKVLRGVLERQAPPAPVEAPEQRNPILPSGDVFFGGNPHMREI
                     HDLVAQIGWSEAPVLIQGETGSGKEVLARALHTQSPRSKKPFLKLNCAALPSELVESE
                     LFGYERGAFTGAFQKKPGMFEMADGGTILLDEIGDMDFKLQAKLLQVLQDQEFQRLGG
                     KETVRVDVRVIAATHRDLEKSIVEETFREDLYYRLNVISLRVPPLRERKEDIIALAEF
                     LLAKHATQGNAPVITPGLKQAFLAYDWPGNVRELENVVRRFIILRSPEKLAADLRRKV
                     AQRASVQSVHAIHTLPDEQRTADATILEQVSKAKQKAEQTAILAALDSTHWNRKKAAL
                     VLKIDYKALLYKMKKLGIEDKMVALAEARDGNEEPAAEPVAGSTA"
     gene            complement(4316..5026)
                     /locus_tag="VFQ79_07395"
     CDS             complement(4316..5026)
                     /locus_tag="VFQ79_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0139516.1"
                     /translation="MSFGGDMQLKNISRVLCLLVLVTGVSFAEFIDFTIPAGGGGGSI
                     SYAGGANPLIGTNLAVSTITGINGVPLNNGVALSCVGCVINFQTGAYTGGSALSWTFA
                     SGGTFTLTGSIPSIAGGAPTTLMSGYFMSDSIVGQLPLPQIPGLGAFTIAGAAFSTIL
                     DPALTNFYGTPADPMRYASNFNISFYTNGSPAGAFSSNAIASGNLTAAAPEPVSIVLL
                     GTALIGCAAIGRRRLKRS"
     gene            complement(5175..7442)
                     /locus_tag="VFQ79_07400"
     CDS             complement(5175..7442)
                     /locus_tag="VFQ79_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0139517.1"
                     /translation="MRVYFAGLVAVILLFSLIACSRSPEEKQARYLASGRGFVAQKDY
                     ARAILEFKNAIEAKPNAPEAYYEIGLAYLATGEWQQAAVNLKRATDLNPGHAGAQIKI
                     SQLMAASGDRALLEEARKRLEGVFQTSPDDVEAMTALAVTEWKLAKPEEAEKYLRRAF
                     EKAPQNLGSSVALARLKAEQKQFDEAEKILKQAVGEPPSAGILVALGEFYYQRNRPLE
                     AAEQFERALRADPKHAPALLDLAAMAVRAGDTAKAEQIYKRISELPDKQYRPMHAVYL
                     FQSGKRDLAIAELRRLHQKAPEDRTVRDHLLAALIWSDRTPDAERLLSEALKKNPDDV
                     DALLQRAGLYLTLRRYGEAETDLSKALPARPDSAETHYLFSKIREARGQILTQRQELD
                     EALRLKPDLLPARLELAALLIRTNHAKIALDVLDRTPEEQKKLAPVAIQRGWALFAMR
                     KTDELEQALKGDLGRLDTPEAHVLSAALRLAQNRMAEARRLAQQALAGNPEDLRALEI
                     LTRGYTEEKNLPEAVRQVAVYAGQRPKSAPLQQFLGSLLLASGDRAKARAAFSRSRAV
                     DPAFLPARLGLVQIDVAEGKWNEAKTSLVQIVSEHGDNLVARLWLGDIEEKAGNASAA
                     IEQYQRVIAGDQDNIMALNNLAFLLADSAGKPDEALKYAEKAVELAPSNPAVVNTLGW
                     VLYQKGLYELAVKRLSEAVKLEGTARRNLHLGMAYLKHGDRNNARAALDTALKLSPPG
                     APEARQAKELMAVLN"
     gene            complement(7460..8866)
                     /locus_tag="VFQ79_07405"
     CDS             complement(7460..8866)
                     /locus_tag="VFQ79_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEU0139518.1"
                     /translation="MMSICHHYVSGKSILLAGLESILFTAALLCGVQIRFWRDPEAVH
                     AYMQNPAFIFQVLAVVLTFQICFYYGDLYNVSALRPPSRQLVSLGNSLGIGCLILGVS
                     YFVFPNLLIGRGVFFISLFFAGTFVLLTRMAVDKAWPVAASGQKIAILGTGGLAQTVA
                     SVIAARGDLGLRLVGFIAAGTEPSRNGNGLEKPALGVVDELEPIIEQCGISRLVIAVE
                     DRRGMLPVNSLVRLRTRGIRIEDAHTAVSSLTGRVWLELVQPSWFIFSNGFSRSPFLL
                     FLKRVADVCFAGVGLAVGWPLMLLIALAIRLDSRGPAIFRQTRVGLNGRHFEVLKFRS
                     MYADAEAHTGARWAAEDDPRVTRVGWILRKFRLDEMPQFVNILRGDMSFVGPRPERPV
                     FVDQLRSQVPYYDQRHSVRPGLTGWAQVEYSYGASVEDAIRKLEYELFYLKNMSAFFD
                     LVIVLKTIRVVLRGRYGR"
     gene            9088..9966
                     /locus_tag="VFQ79_07410"
     CDS             9088..9966
                     /locus_tag="VFQ79_07410"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEU0139519.1"
                     /translation="MATTAECAQTEQQQPQSQSASWPGYLLIGVLIAALYGYVLYDLG
                     RDWWFEPSLSQGLLIPPLALFVAWSRRELTLALPAVPDWRGVLLASVACLFYLAGKLG
                     AEFFLPRISFVILLAALVWTFWGVQRLRTLTFPLLLLATMVPLPSILYNTISAPLQLL
                     ASDAASRVIELTGVTVHRDGNMIHLADITLGVEEACNGLSSLSTLLVASLLLGFILCS
                     RPAARWLVFLAAVPVAIATNILRIAGTAWIADYRVELALGFYHLFSGWLVFLVGFTGL
                     YAVAKISNAILPGAPK"
     gene            9963..10613
                     /locus_tag="VFQ79_07415"
     CDS             9963..10613
                     /locus_tag="VFQ79_07415"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEU0139520.1"
                     /translation="MKPTSSRAVAALCLLLAATLAASRLSERRVPEYLAQPLDSIGTE
                     LAGWRAAQELSVDDKVLGQLRPTSLLSRLYRKDQDELGLFIAYYAEQRAGESMHSPKH
                     CLPGSGWEIWKRNSVEVPFEDRMVTINRYAIRKGMQRMLVLYWYQSRSRVIANEYTGK
                     VLLVRDALLDGRTAGSIVRINIADNPHSEEAGLIFAKAVMAELQRCLKPRRAPEQT"
     gene            complement(10610..11497)
                     /locus_tag="VFQ79_07420"
     CDS             complement(10610..11497)
                     /locus_tag="VFQ79_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0139521.1"
                     /translation="MIDAGGHRLHLNCTGKGSPAVLIEPGLGTDWISWQAVRQKISQF
                     TCVCVYDRAGYGWSDSGPFPRTSGQIAAELELLLKNSGIAGPYILAAHSFGAYDARIY
                     AARNPAQVAGLVLVDPSHEDEARLSPPPQEPAPVEHPFGLRDLLQLVPPLGTDHIIRL
                     WKGRAILPPELSDAPPDVQNRFVMGTPLRQRDAEISELASLSESEAQVARASLPRNMP
                     LRVLTSMKLESPRTHKESVPDVSRIHIEVQARLAASSTNGEQIRAHASGHFIQLDEPE
                     LVVRAIQDVVEQSRRNRTN"
     gene            complement(11852..13429)
                     /locus_tag="VFQ79_07425"
     CDS             complement(11852..13429)
                     /locus_tag="VFQ79_07425"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEU0139522.1"
                     /translation="MNGTNHGVSRRGFLGRAGALASVAGSLEVLHAASKPGAQPKNII
                     FMVADGMSPSVLPLAEHFSQHVRGKGLLWRALVDRPEAARALMDMASLNSVTTDSSAA
                     SSSWGSGVRILNGYVNSLPDGTELTPLAAIARDRRKRVGLVTTTTVTHATPAGFAATE
                     KSRGNEDGIAVQYLAKADVILGGGRQFFEEATRKDKADLIERYRQASFSYVRSRSELK
                     ALPAKTSARVLGLFSPGMIPYTLDRNNSVELQESVPTLAEMTSAALDALKSSPKGFLL
                     QIEGGRVDHAAHNNDTAALLWEQLAFDEAIEVALRFAEHSPLRQQCRPGTLADIEVAL
                     RFAEHSPETLIVITSDHGNSNPGLGGKWAELNGASNGFARLAKAKSSFAELMPKLDGK
                     QKLASSAVHELMREACGCEFAEDEVEAVRSAAAGVRKLSLNSRLDKLAGILGQAVGNH
                     TGIGWVSTDHTSDFVLLTALGPGSKLFNGQIRNTKCFDNIVDLMGSKFRNPMMTFEQA
                     KRFLRASAARHESRHWV"
     gene            13546..14388
                     /locus_tag="VFQ79_07430"
     CDS             13546..14388
                     /locus_tag="VFQ79_07430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0139523.1"
                     /translation="MPEETTTEFQKEVREGQRFEFGKNWQQFLRELTEERIQAAAASL
                     KSNLEVETLRGLTFLDVGCGSGLFSLAAHRLGALVHSLDYDPHSVACALELRRRYGSE
                     DLPWTIEQGSALDSEYLRSLGTFDVVYSWGVLHHTGSMWAALENMVPLVAPRGKLYIA
                     IYNDQGTASRRWLAAKKLYNEKTWLRPLLSGLCLVYVWWRPLVKDVLRGRPGHSWRDY
                     RRRRGMSPWRDVVDWIGGYPFEVAKPEEIFQFYRGRGFVLEKLTTCGGSLGCNEYVFR
                     LGDS"
     gene            14491..14766
                     /locus_tag="VFQ79_07435"
     CDS             14491..14766
                     /locus_tag="VFQ79_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU0139524.1"
                     /translation="MSKRLVIDRAGRVVIPKPVREELHLEPGDSLEMESTGEQITLRP
                     VRGTGPLTKEHGVWVFYSDEPLAASVTGDMLQQIREERDLANLGKGE"
     gene            14763..15188
                     /locus_tag="VFQ79_07440"
     CDS             14763..15188
                     /locus_tag="VFQ79_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0139525.1"
                     /translation="MKGFFDTSVLVPVFYGNHVHHRASLDLFIQFDKSTGCCGAHSLA
                     EVYSTLTRMPGKHRIGAEQAMLFIGSIRERLSIIALTGDEYADALQAASVRGIVGGGI
                     YDALLAHCAIKAQAETIYTWNVRHYSLCASEVTQRLRTP"
     gene            complement(15185..17188)
                     /locus_tag="VFQ79_07445"
     CDS             complement(15185..17188)
                     /locus_tag="VFQ79_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0139526.1"
                     /translation="MALPIRLLVYGGVCATALLVLASCRSANQKRTEEAAPLRRMNLV
                     LVTIDTLRADRLGCYGANAETPHLDKLAQRGVLFENAIAQAPLTAPSHASMFTGLYPT
                     AHKVRDTGGFILPSQHTTLAEVLQAQGWDTAAFVGASVLKQRFGFNQGFAVYDDEMDA
                     GGAASEAPERRAEEVVRRAIQWLDAQSGKPYFLWVHVFDPHLPYDPPSPFREQYRGRP
                     YDGEVAYTDRELGRLLDRIESKSSGNTLIAVLSDHGESFSEHGEYAHGIFLYDTTLRI
                     PFLLAGPGVPPGVRKKQQARSIDLLPTVLELMGGKAPAEVQGASLVPAFNSEEAPAAY
                     AYVETLFPKINMGWTELRGIRTNQWKYIRAPRPELYDLVHDPGESTNVIAAHAEKARE
                     LEAQLKTLSPEVEKVESGASDRRTLEQLRSLGYLGGSSQREFTLTGKGVDPKDRIEVL
                     RLLHFAVYADSKIPPSKSVALLRQAVALDPANPTLYTHLGDVYRRAGRRSEEIHLYED
                     GIAKGIRNASLYSRLGALYLQQGNKEKAIADFEQAAQLNPHDYESLQNLAVAYRGTGR
                     VADAERVLNAIIASGEEFAPAYNELGMVAFQKGEIAKAREHFEKAARLDAAYQLNLGR
                     LYRMAGEGDKARHAYEAFLASADNRPEYRDLVREARKELALIR"
     gene            complement(17259..17903)
                     /locus_tag="VFQ79_07450"
     CDS             complement(17259..17903)
                     /locus_tag="VFQ79_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0139527.1"
                     /translation="MSLNTCCIIIFLCLAPSAICQHLSVAIGGGAGPASGNARTDPAG
                     RDAKPETGGTLLLNAGLEFLHHKSVSIGIDVPVALHGSRSSDVFASGFYASIYTERLT
                     AALTPGFRVRFGPGRRLSPWLSFGAGLGTIRRTGTDFQSGQQTASQSDSNLTVVLTPG
                     AGADIRVARRWFLRGELRNYLYRTPATGFVSSFTYWNRWNYNPVVAGSVGFRLP"
     gene            complement(18019..18603)
                     /locus_tag="VFQ79_07455"
     CDS             complement(18019..18603)
                     /locus_tag="VFQ79_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139528.1"
                     /translation="MANANEGFLVLPRLVPPTTAVLDRPFIAAFHTVFVEKLKEPRLA
                     CAQGRQYRNSAGITKIELLQPNAPEKGVTFAQRWNPSDRKMEVYVEGKQSVWPPSIEE
                     VWLPRGGTGPLVHMKRTDESRLIHGQECRRIQLWLPEEDPNLITNEVWICEELAIVVS
                     DMVETRTQLSHWELNCIDLQEPDPESLDLPGENR"
BASE COUNT         3995 a         5782 c         5649 g         4010 t
ORIGIN      
        1 cttcgcgccc ggcaccggcg ccggagatga tacgatccag ttctccacgg ggggccgcac
       61 ggtggatttc agcattcccg cgggcagcac ggcggctgtt ttctcctcac cgcaggtggc
      121 tcttcagacg ggcaccgtgg cgggaaccat caccgtagcc gcccgcctgg cttccgccgg
      181 tgtggagatc acgccttcct cgccacccac acacgccata cgtatcgagc agtctcggcc
      241 aatgctcacc agtgcccgcc ttacgcggac tgcaacgggt ttcagcatcg aaattaccgg
      301 ctattcgacg gcgcgtgaag tgacgcaggc ggtatttcgt tttggagccg ctcccggcca
      361 gagactggaa cagccggaaa tcacgatgga tgtgagaaac ctgtttaccg cgtggtttga
      421 agacgccgca tccgcgccgt tcggcagcca gttcacgttc acacaaactt tcaccgtaag
      481 tggagacgcc gcggcagtta ctccgcaaac tgtcgtactg cgtaacagcc tgggcgaaag
      541 tagtatcact gtgcagtaac tgcgggaagt tgtgccgggc gtgtgtacgg gggatcgcgc
      601 aaagggcggc cggtgagcac gaaggttcgc cggtcctcag acccgcgagg aggttatcgg
      661 agtctcttcc cgctggatca cccgatagtg ttcgatgcgg caggcgaggc caaaacgggc
      721 ggagcggccg atggtttcaa cgcgaacgac gagcgcctgg catcgcagac acaaaaaagt
      781 atcttttttt tccttattgt gtgaaggaaa caggatgtcg cagtcgatat gttctccagg
      841 tgtcaatggc tcctggacga tgcaatagaa accctcgctg ctcagattca cggtccggct
      901 ttccattgga tgcgtctcag acctccggca aagatagacc ggcaactgca gcggcaggcg
      961 cgtgcgcctg cgcctttcag tcagaggtcc ctgcctctct tcgtcatctg aggtcatcag
     1021 cacggcaccg tacgcagaac aacattccaa ggagaaagac atgtaagccg aacggacacc
     1081 gcgtgaatgt gcacttttgc taccacgcac aaccggctgg aactgggcaa ctccgttgca
     1141 gaatgtacga aagtataggc ccaaagttcg gggtacgaat gggctatacg gatgaatttt
     1201 tggggcgaat cgtcgaacaa cggcatcgga agttgtcaag tcctaaaggt tgggactaga
     1261 acttacaaga tggtgttcta gtaccagcaa ccatttgtaa atcattgaca ataagtggga
     1321 ctacgctatg cttgatggca acttaataaa actccggact catcgcactt agacctttcg
     1381 ggtctcctga atgtgccggt cctgccacag ggagggctaa cgggacggca aaatagcgga
     1441 ttttgtgctc ttttgtttca cggttttgaa atatgtctat gaacgttttg ctggtttcaa
     1501 acgatgctga gctgcagaag ctgatccggg cggtattggc cgagtgcctg ggtgcggcct
     1561 ggcggctggt gattgctacg cagcaagact taacccggaa ggcggatgtg tatatctggg
     1621 actatcactc cgggctgcgc atgagcgatt tcatcgatcc ttccgagcat caaaaacact
     1681 ttgtgctggt gcagcgcaaa caactttcac agttccgcga ggaatctctg ccggatgtca
     1741 atgttgttct gaagccggtc tccgccgctg cgctgaaggc gtttctcggc gaagcttgcg
     1801 cccgctggca gcgccagcag gcagaacacg aggacgaggc cggcagcgtg cgggccgacc
     1861 gcgatgaagt actgcaaagt ttgatcgagg ccaatctcaa attacaggag tacgaccagg
     1921 accggactaa ctttctggcc cgggcgattc acgatttccg tgccccgctg acagccctga
     1981 cagggtattg cggcctcctg ctgggggagc agttaggcgc attgaccgcc gaccagcggg
     2041 aggtaatcca gcgcatgcag ttcagcgcca agcggctgtc gcgtctggcc agcgaaatgt
     2101 ttcaactgag tatctgccac cgtgttgtta cgcggcccaa cctccggaga cacgacatcc
     2161 acgaatgcgt agaccaggcg ctgcacgaaa tcgctccgtt tgccgagaca aagcgcatct
     2221 ccgtgtccgt cgacctgcaa cagccggccg agcccttggc tttcgagccg agtgaactcg
     2281 aacaggtact ggtgaacctg ctggacaacg cgtgcaagtt tactccgaga tccggttcca
     2341 tcgacgtcaa gggatatccg ttcttctggg agcgcaggaa ggacggaatt acggaaatgc
     2401 cgttgcgcgc cgaccggcgg accagcaacg gccatgcgcc gaactcgttc cggatcgata
     2461 tttccgattc gggtcctggc attcctccga accacatcga aacgattttc gaggagtata
     2521 cctcgtatgc gggagggcag gaccggtcag gcggcggcct ggggctggcc atctgcaaga
     2581 tgattatgaa ccggcaccat ggacgcattt gggcggaaag cagtccgagg ggcgccgttt
     2641 tctcgctggt gctgccttct cctgcggccg aacatctgct cgccaccgat gcggcgcctt
     2701 ccgagaacaa gtactacacc caagtgagtt gagatatgca agcgataaaa aacggccgca
     2761 cgattctggt gggtgaagac gagcctgaag ttcgcagcta cctggagatg gccttgcggt
     2821 gccagggcta cacggtcgaa ctggccgaaa acggcgacga ggtgctggcc tgtcttcagg
     2881 attcgggcga gcacatctcc gcagtcctgc tggacattgt catgccgcgt aaagacggcc
     2941 tcgaaacgct gaaagagatc cgccgtatcc gggacgatct ccctatcatc atggtatccg
     3001 gcgcgtcctc accgctgaat gtcgtggagg cgatgaagaa cggggccacc gatttcatcg
     3061 gcaagccggt caaccacgaa gacctgcgga aagtgctgcg aggcgtgctg gaaagacagg
     3121 ctccaccagc tccggtcgaa gcgcccgagc agcggaaccc gatattgccg tcgggagatg
     3181 tgttttttgg cggcaatcca catatgagag agattcacga cctggtcgcg cagatcggct
     3241 ggtctgaagc gcccgtattg atccagggcg aaacgggctc tggaaaagag gttctggcgc
     3301 gggcgctcca tacgcagtct cctcgctcca aaaaaccatt tttgaagctt aattgtgccg
     3361 ccctgccttc cgaactggtg gaaagcgaac tgttcggtta tgaacgaggc gctttcaccg
     3421 gtgcgttcca gaagaagccc ggcatgtttg aaatggccga tggcggtacg attctgctcg
     3481 atgaaatcgg cgacatggat ttcaagctgc aggcaaaact ccttcaggtc ttgcaggacc
     3541 aggagttcca gcgtctcggc ggcaaggaaa ccgtgcgggt ggatgttcgg gttattgccg
     3601 ccacccatcg cgacctggag aaatccattg tggaagagac gtttcgcgaa gacctgtatt
     3661 accggttgaa cgtcatcagc ctccgtgtgc cacctttgcg cgaacggaag gaagatatta
     3721 tcgcgctggc ggaattcctg ctggcaaaac acgccacgca gggaaacgct cccgtgatca
     3781 cgccaggcct caaacaggca ttcctcgctt atgactggcc cggcaatgtg cgtgaactcg
     3841 agaatgtcgt ccgccggttc attattttgc gaagcccgga gaaattggct gcggatcttc
     3901 ggcgcaaagt tgctcaaagg gcatcggtgc aatcggtgca cgccatccat accttgcccg
     3961 acgaacagcg caccgctgat gcgaccattc tcgaacaggt gagtaaagcc aagcagaagg
     4021 cggaacagac ggccattctg gcagcgctcg attccactca ctggaaccgt aaaaaagccg
     4081 ctctggtgtt aaagattgac tacaaagcgc tgttgtacaa gatgaagaag ctggggattg
     4141 aggacaaaat ggtggcgctg gcggaggcgc gggatggaaa tgaagagccc gccgctgaac
     4201 ccgtggccgg tagtaccgcg tgagtgcgct atagcaagac caccggaaca aaaaagcagg
     4261 ggctccaccc aagtggaaca tcgcccctgc caactaccga ctgttcaaga tcaactcagg
     4321 atcgtttcag acgccttctt ccaatagccg cacatccgat cagcgccgtg ccgagcagca
     4381 cgatcgagac cggttcggga gcggcggcgg tcaggttccc gctcgcgatg gcattgctcg
     4441 agaacgcacc cgccgggctg ccgttggtat agaacgagat attgaaatta ctcgcgtacc
     4501 gcatcggatc ggccggcgtt ccgtagaagt ttgtgagcgc cgggtcgagg atggtgctga
     4561 acgcagcgcc ggcgatggta aatgcgccca ggccggggat ctgaggcaga ggcaactgcc
     4621 ccacaatgga atccgacatg aaatagccgc tcatcagcgt cgtcggcgcg ccgccggcaa
     4681 tagaggggat cgaacccgtc agcgtaaagg ttccaccgga agcaaaagtc cagctcagcg
     4741 ctgatccacc cgtataggct ccggtttgaa aattgatcac gcacccgaca caggacaggg
     4801 caactccgtt attcagcggc accccgttaa tccccgtgat ggtgctgacg gccagattcg
     4861 tgccgatcag gggattggcg cccccggcat acgaaatcga accgccgccc ccgcccgccg
     4921 gaatagtaaa atcgataaac tcagcgaaac tcacgccggt caccaacacc aaaaggcaga
     4981 gtacccggct tatgtttttc aactgcatgt cgcctccgaa agacatcgtt gtagagaata
     5041 caccttaatt cgcattaagg aattgcacgt agcactccaa agttgcatct gttgcgctgc
     5101 ttatagttac gtaccacata acccctggat agtgcgaaaa gcccgtatgt ttggagattt
     5161 tgtcgtataa acccttagtt cagaaccgcc atcaactctt tcgcctggcg ggcttcgggt
     5221 gcgccggggg gcgacagttt caaggccgta tccaacgccg cacgggcatt gttccggtcg
     5281 ccatgcttta aataagccat acccagatgc aggttccggc gggccgttcc ttcgagcttt
     5341 accgcttcgg aaagccgttt tacggccaac tcatacaggc ctttctggta cagtacccag
     5401 cccagcgtat tcactacggc gggattggac ggagctaatt ccaccgcctt ctccgcatat
     5461 ttgagcgcct cgtccggttt gccggcgctg tccgccagaa gaaatgcgag attgttcagg
     5521 gccatgatat tgtcctggtc ccccgcaatc accctctgat actgttcgat cgcggccgag
     5581 gcgttgcccg ctttttcctc gatgtccccc agccagagcc gggccaccag attgtcgccg
     5641 tgctcggaaa caatttgcac caggcttgtt tttgcttcat tccatttgcc ttcggcgacg
     5701 tcgatctgta ccaggcccag ccgcgccggg aggaaggccg ggtccaccgc gcgggaccgg
     5761 gaaaaagcag cccgggcttt ggcgcgatct ccggacgcca gcagaaggct gcccagaaac
     5821 tgctggagcg gcgcggactt cggccgctgg ccggcgtaca ccgccacctg ccgcaccgct
     5881 tccggaagat tcttttcttc ggtatagccg cgggtcagaa tctcgagcgc ccgcaggtct
     5941 tccggatttc ccgccagcgc ctgttgagcc agccggcgtg cctccgccat gcggttctgc
     6001 gccagccgca gcgcggccga aaggacatgc gcctccggcg tatcgaggcg gcccagatct
     6061 cccttcagcg cctgttcaag ctcgtccgtc ttcctcatcg caaacagcgc ccaaccgcgc
     6121 tggatcgcga ccggcgccag tttcttttgt tcctccggag tcctatccag aacatcgagg
     6181 gcgattttgg cgtggttggt ccggatcaaa agcgccgcga gttccagccg tgccggcaac
     6241 aaatccggct tcagccgcaa ggcttcgtcc aactcctggc gctgtgtcag gatctggccg
     6301 cgcgcctcgc gaattttcga aaacagatag tgcgtttccg ccgagtccgg ccgggcggga
     6361 agggctttgc tcaagtcggt ctccgcttcg ccgtagcggc gcagggtgag atacaagccg
     6421 gcgcgctgca gcagcgcgtc cacatcgtcc gggtttttct tcagggcttc cgataacagc
     6481 cgctccgcgt ccggcgtccg gtccgaccaa atcaacgcgg ccaggagatg atcgcgcacc
     6541 gtacggtctt cgggggcctt ctggtgcagg cggcggagtt cggcaatggc gagatccctc
     6601 tttcccgact ggaacagata taccgcatgc atgggccggt attgcttatc cggaagctcg
     6661 gagatccgct tgtagatttg ctccgctttg gcggtatcgc ccgcgcggac cgccatcgcc
     6721 gccagatcga gcagcgctgg tgcgtgcttc gggtcggcgc gcagcgcgcg ctcgaactgc
     6781 tccgcggcct ccaacggccg gttgcgttga taataaaatt ccccgagcgc caccagaatg
     6841 ccggccgacg gcggttctcc caccgcctgt ttcagaattt tttcggcttc gtcgaactgc
     6901 ttctgttccg ctttcagcct ggccagcgcc acggaagaac ccaggttctg gggcgctttc
     6961 tcaaaggcgc gccgcagata tttttccgcc tcttccggct tcgccagttt ccactccgtg
     7021 acggccaggg cagtcatcgc ctccacatcg tccggagagg tttgaaacac tccttcgagc
     7081 cgcttgcggg cttcttcgag aagcgcccga tcgccggagg ccgccatgag ctgcgaaatt
     7141 ttgatctgcg ctccggcatg cccgggattg agatcggtgg cccgcttcag gttcaccgcc
     7201 gcctgctgcc attcgcccgt agccagatag gcaagaccga tttcgtagta cgcttccggg
     7261 gcattcggct ttgcctcgat cgcgttcttg aactcgagaa tcgcccgggc atagtctttt
     7321 tgagccacga aaccccggcc tgacgccagg tatcgggctt gcttttcttc cggagatcgc
     7381 gaacacgcaa tcaggctgaa aagaagaatc actgccacta aacctgcaaa ataaactctc
     7441 atgattagtc tccgccgcat catctgccgt accggccgcg cagcaccacg cggatcgttt
     7501 tcaaaacgat caccagatcg aaaaaggccg acatattctt gaggtagaag agttcgtatt
     7561 cgagttttcg aatcgcatcc tccacgctgg cgccgtagga atattcgacc tgggcccacc
     7621 ccgtcaaccc aggccgtacc gaatgccgct ggtcgtaata agggacctgg gagcgaagct
     7681 ggtctacaaa aaccggccgc tccgggcgcg gtccgacaaa actcatatcg ccgcgaagga
     7741 tattgacgaa ttgcggcatt tcatccaatc ggaattttcg taaaatccag cccacccggg
     7801 tgacgcgcgg gtcgtcttcc gccgcccagc gggcgccggt atgcgcttcc gcatccgcat
     7861 acatcgaccg gaatttcaat acttcaaaat ggcgtccgtt caggcctact cgcgtctgcc
     7921 ggaagatcgc cgggccccgc gagtccaacc ggatggccag ggcgatcagc agcatcaagg
     7981 gccatccgac cgcgagtccc acgccggcga agcacacatc ggcaacccgc ttcaggaaga
     8041 gaagaaaggg agaccggctg aagccgttcg aaaagatgaa ccagctcggc tgaaccagtt
     8101 ccagccatac gcgccccgtc agggaagaga cggccgtatg agcgtcctct atgcggattc
     8161 cccgtgtgcg cagcctcacc aggctgttta ccggcagcat gccgcgccgg tcttccaccg
     8221 cgatcaccag ccgcgaaatg ccacactgtt cgatgatcgg ctccaactca tccaccacgc
     8281 ccagagccgg cttttccagg ccattgccgt tccgggacgg ctccgttccg gccgctatga
     8341 accccaccag gcggagcccc aggtcgccgc gagcggcgat gaccgaggcc accgtttgcg
     8401 ccagcccgcc agtcccgaga atggcgattt tttgaccgga ggccgcgaca ggccaggcct
     8461 tgtccaccgc catccgtgtg agcaacacaa atgttccggc gaagaatagc gaaatgaaaa
     8521 acacgccccg gccgatgagc agatttggaa atacgaaata acttactccc aatatcaggc
     8581 agccaatccc gagggaattc cccagtgaaa cgagctggcg ggatggtggc cgcaacgcgc
     8641 tgacgttata cagatcaccg taataaaaac agatctgaaa ggtaaggaca accgccagaa
     8701 cctggaaaat aaaggccgga ttctgcatgt aggcgtgaac cgcctccggg tctctccaaa
     8761 agcggatttg cactccgcat agcagggccg ctgtaaacag gatgctttca aggcccgcca
     8821 gcaggatact tttgccggag acgtagtggt ggcagatcga catcatgcaa gacgcataag
     8881 acagtaaaca cttgcatcgc aatccccttt ccaaaacctc taccgccgtc aacgctgcaa
     8941 aaacaggggg aatacgagct gctgtgcgct tttttgtcaa agaatacaag ccttttgccc
     9001 aatagatctg gaaatgcttc gaacgccatg gaaaacgaat ggtttgagct ggtcggccct
     9061 gtgcgtgcat cgtggatgag ctgacggatg gcgacgacgg ccgagtgtgc gcaaacggaa
     9121 caacagcagc cccaaagcca gtctgcgtcc tggcccgggt atctgctgat tggcgttctg
     9181 atagcggcac tctacggata tgttctgtat gatctggggc gcgactggtg gtttgagccg
     9241 tctctgtcgc agggactcct gattcctccc ctcgcgctgt ttgtcgcctg gagccggcgc
     9301 gaattaacgc tggcgctgcc tgcggtaccg gactggcgcg gcgtgctgct ggcgtccgtg
     9361 gcctgccttt tttatctggc cggaaaactc ggagcggaat tcttcctccc gcggatttcg
     9421 tttgtcattc tgctggccgc tctggtgtgg acgttttggg gcgtacaacg cttgcgcacg
     9481 ctcacgtttc ccctgttgct gctggcgaca atggtgccgc tgccgtcgat tctctacaac
     9541 acgatctcgg cgccgcttca actgctggct tccgatgcgg cttcgagagt catcgaactc
     9601 acaggagtaa ccgtacaccg cgacggaaac atgatccatc tggcggatat tactttgggg
     9661 gtcgaggagg cctgcaacgg cctcagttcg ctgtcaacgc tgttggtggc cagcctgctt
     9721 ctcggcttta tcctctgctc gcgccctgct gcgcgctggc ttgtttttct tgccgccgtg
     9781 ccggtcgcaa tcgccacgaa tatcctgcgg atcgcgggca ccgcctggat tgccgattac
     9841 cgcgtcgagc tggcgcttgg tttttatcac ctgttctccg gctggctggt tttcctggtg
     9901 ggcttcaccg gactgtacgc ggtcgctaaa atttccaacg ccatcttacc cggagcgccg
     9961 aaatgaaacc cacctcctcg cgggccgtgg ccgcactctg tctgctgttg gctgcgacgc
    10021 ttgccgcttc cagactcagt gaacgccgcg tgccggagta tctcgcgcag ccgctcgatt
    10081 cgatcgggac ggaactggcc ggatggcggg cggcgcagga actgagtgtc gatgataagg
    10141 tcctcggaca gctccggccg acgagcctgt tatcgaggct ctaccggaaa gatcaggatg
    10201 agctgggcct gttcatcgcc tactatgccg agcagcgcgc cggcgagagt atgcattccc
    10261 ccaagcattg tcttccggga tcgggatggg agatctggaa acgcaattcc gtcgaggtac
    10321 cgttcgagga caggatggtc acgatcaatc gctatgcgat ccggaaaggg atgcagcgga
    10381 tgcttgtgct gtattggtac cagtccaggt cgagagtgat cgccaatgaa tatacaggca
    10441 aggttcttct ggtgcgcgat gccctgctgg acggacgcac tgccgggtcg atcgtccgga
    10501 tcaacattgc cgacaacccg cattccgaag aagcgggcct gatcttcgcc aaggcggtaa
    10561 tggccgagct ccagcgatgt ttgaagcccc ggcgggcgcc tgaacaaact tagttggtcc
    10621 tgttgcggcg cgactgttcc acaacatcct gaatggcgcg gacaaccaac tccggttcat
    10681 ccagttgaat gaagtgtccg ctggcgtgag cccggatctg ctccccattg gtcgaggaag
    10741 cggccagccg cgcctgcacc tcgatatgga tccgcgaaac atccggtacc gattctttat
    10801 gcgttcgggg gctttccagc ttcatggaag tcagcacccg tagaggcatg ttgcgcggca
    10861 gactggcgcg ggctacctgg gcttcactct cagacaggga ggccagttca cttatttccg
    10921 cgtcccgttg acggagcggc gtgcccatca cgaagcggtt ctgaacatcc ggaggagcat
    10981 cgctcaattc cggcggcaga atagcccggc ccttccaaag gcgtattatg tgatccgttc
    11041 cgagcggcgg aaccagttgc aatagatctc tgaggccaaa agggtgttca accggcgcgg
    11101 gctcctgcgg aggcggagac aggcgcgctt cgtcttcatg gctgggatcg accagcacca
    11161 accccgctac ctgcgcgggg tttcgcgccg cataaatacg cgcgtcgtaa gctccaaacg
    11221 aatgggcggc cagtatatac ggacccgcta ttccagaatt cttcaggagc agttcgagtt
    11281 cagcggcgat ctggccgctg gtacgcggga acggccccga atcactccac ccataaccgg
    11341 cacggtcgta cacgcagacg cacgtgaatt gcgatatttt ctgccgcacg gcttgccagg
    11401 atatccagtc tgttcccaga cccggttcga tcagaactgc cgggctgcct ttgccggtgc
    11461 aattcaaatg caggcggtgg cctccggcat cgatcatttc tccaggcgga gggtattgct
    11521 ccgttaatac aaaagaaaaa gttttactgg cgccgcgaat gatggggatg cttaaaagca
    11581 acccggctat gaggaaaagt cccattaaag agaggcgcgg caatgacaga gcgggacgga
    11641 gcgatcggcg cataggctga tagggcaatg caaaattgac ccctgggcta caggaacaag
    11701 cccgtgagcg cggatcggag aaaacttcac cgaggggcct ggaaggcttt tccggtgagg
    11761 ctaaacgcct ctttctccgg ggcgcacatc agtattgtgc cgtcagaaat ttagagtgaa
    11821 acagacccga aggtactaga acgagtggcg cttataccca gtgacgcgat tcgtgccggg
    11881 ctgccgacgc acgcagaaac cgcttggcct gctcgaaagt catcattggg ttccggaact
    11941 tcgagcccat caggtccacg atgttatcga agcacttcgt attgcggatc tgcccgttga
    12001 agagtttgct gcccggcccg agtgctgtca gcaggacaaa atcggatgta tgatccgtac
    12061 tcacccagcc gattcccgtg tgattgccaa ccgcctgtcc cagaatgccg gcaagtttgt
    12121 cgaggcgtga attgaggctg agtttccgca cgccagccgc cgcggaccgc accgcttcca
    12181 cttcatcttc ggcaaattcg cagccgcagg cttccctcat taactcgtga acggctgagg
    12241 aggcgagctt ttgtttgccg tccagtttcg gcatcagttc agcaaaggag ctcttcgcct
    12301 tcgcgagcct ggcgaagccg tttgatgccc cgttcagttc cgcccatttt ccgccgagac
    12361 ccggattcga atttccatga tcggacgtga taacaatcaa ggtctcggga ctgtgttcgg
    12421 caaaccgcag agccacttcg atatcggcaa gcgttccagg ccgacattgc tgtcgcaggg
    12481 gactgtgttc ggcaaaccgc agagccactt cgatggcttc gtcgaaggcg agttgttccc
    12541 ataacagcgc cgccgtgtcg ttgttgtgcg ccgcgtggtc cactcgtccg ccttctattt
    12601 gcagcagaaa tcctttcggt gaagatttca gtgcgtccaa tgcggcggaa gtcatctcgg
    12661 ccagcgtagg cacggattcc tgtaactcga cactgttgtt gcgatccagc gtgtacggaa
    12721 tcataccggg ggagaatagc cccagtacgc gggcggatgt ctttgcaggg agcgccttca
    12781 actcgctgcg ggaacggaca taggagaagg acgcctgtct gtagcgttcg atcagatcgg
    12841 ctttgtcttt gcgggtggcc tcttcgaaaa actgtctgcc gccacccaga atgacgtctg
    12901 cttttgccag atattgaacg gcaatgccgt cttcgttgcc acggctcttc tcggttgcgg
    12961 cgaatccggc cggcgtggca tgcgtcaccg tggttgtggt gacgagcccg acgcgttttc
    13021 tccgatcccg ggcgatggcg gcgaggggcg tcaactcggt gccgtccggc aatgagttga
    13081 catagccatt gagaatccgc acgccggatc cccaactgga cgaggcagcc gaggaatccg
    13141 tcgtcaccga gttcaacgac gccatatcca tcaaggccct tgcggcttcc ggacggtcga
    13201 ccagagcgcg ccacaacaac ccctttcccc ggacgtgctg ggagaagtgc tccgccagcg
    13261 gcaggacgct ggggctcatc ccgtcggcga ccatgaaaat gatgttcttc ggctgtgcgc
    13321 caggtttgct ggcggcatga aggacctcga gcgatcccgc tacggaagcg agcgctccgg
    13381 cgcgaccgag aaatccgcgc ctgctgacgc catggttggt tccgttcata tcggtacttt
    13441 cgtaggttta tatcttgttc acaacataac ataggcgcct cagacgtccc ctgtctctgg
    13501 tttgattccc tcgcgaaatt cgcagtacgc tacgatttgt gctccatgcc cgaagaaact
    13561 acgacagaat tccaaaaaga agtccgggag gggcagcgct tcgaatttgg taagaactgg
    13621 cagcagtttt tgcgggaact taccgaagaa cgcattcagg cggcggccgc ttcactcaag
    13681 agcaatctcg aagtggagac tcttcgcggc ctgacgttcc tcgatgtcgg ctgcggcagc
    13741 ggattgttca gcctggcggc gcaccggctg ggcgcgcttg tgcattcgct cgactacgat
    13801 ccgcactccg ttgcctgcgc gctggagttg cggcggcgct atggctcgga agatctgccc
    13861 tggaccatcg aacaggggtc ggctctcgat tcggaatatt tgcgctcact gggaacgttc
    13921 gacgtggtgt attcctgggg cgtcctgcat catacaggct cgatgtgggc cgcgctggaa
    13981 aatatggttc cgctggtggc gccccggggc aagttgtaca tcgccattta caacgaccag
    14041 ggcaccgcga gccgccgatg gcttgcggca aagaagctgt acaacgaaaa gacctggttg
    14101 aggccgctgt tgtctggcct gtgcctggtt tacgtctggt ggcgtccact ggtgaaggat
    14161 gtgttgcggg gccggccagg gcactcctgg agagactacc ggcgccggcg cggcatgtcc
    14221 ccctggcggg atgttgtcga ttggatcggc ggctaccctt tcgaggtggc caagcccgaa
    14281 gaaatcttcc agttttaccg cggtcgcgga tttgtgctcg aaaagctgac gacgtgcggc
    14341 ggctcgctgg ggtgcaacga atacgtgttc aggctggggg atagctagcc acagccatcg
    14401 ctgcgctcga tgggaaacgg cggttcgcga ctcggctacc cagtctacaa tggtattaac
    14461 tgatggttcg acccacagta ttgtgccaaa atgagcaaac gcctagtgat cgatagggcc
    14521 ggtcgcgttg tgatccctaa gccagtaagg gaagaactgc atttggagcc cggtgattcc
    14581 cttgaaatgg agagtaccgg cgagcaaata actctgcgcc cggtgcgcgg cacaggtccg
    14641 ctcaccaaag agcatggtgt ctgggtgttt tattccgatg agcccctggc cgcttccgtc
    14701 accggcgaca tgctgcaaca gatccgcgag gaacgtgact tggcgaacct cggcaaaggt
    14761 gaatgaaggg atttttcgat acgtcggtgc tggtgccggt gttctatggc aatcacgtcc
    14821 accacagggc gagtctcgat ctcttcattc aattcgacaa gtcaacgggc tgctgcggag
    14881 cgcacagcct ggcggaagtc tattccacgc tcacgcgaat gccgggcaag catcgcatcg
    14941 gcgcagagca ggccatgctc ttcattggca gcatacgcga gcgactctcg atcatcgcgc
    15001 tcaccggtga cgaatatgcc gatgcgttgc aggccgcctc tgtccgcggc atcgtgggcg
    15061 gcggaattta tgacgccttg ctcgcgcact gcgccatcaa agcccaggcg gaaacgatct
    15121 acacctggaa cgtgcgccat tattcgctgt gtgcttcaga agtgacgcag cgactgagga
    15181 caccttagcg gatcagagcc agttctttgc gagcttcccg cacaagatcg cgatattccg
    15241 gccggttgtc cgccgacgcc aggaatgctt cgtaggcatg gcgagccttg tccccttcgc
    15301 ccgccatccg gtatagccgg cccagattga gttgatacgc cgcatccagc cgggcagcct
    15361 tttcgaagtg ctcgcgcgcc ttggcgatct cccccttctg aaatgcgacc atacccagtt
    15421 cgttgtatgc gggagcgaat tcctcgcccg aagcgataat cgcgttcaat actctttccg
    15481 catcggcaac acgtccggtt ccccggtacg ccacggccag attttgcagg ctttcataat
    15541 catgcgggtt caactgtgcc gcctgttcaa agtccgcaat cgccttctcc ttgttcccct
    15601 gctgcaaata gagcgcaccg agccgggagt acagagaagc gttgcgaatt cctttggcga
    15661 tgccgtcctc gtacaggtgg atctcctcgc tccggcgccc ggcccggcgg tagacatcac
    15721 ccaggtgcgt atagagcgtc gggttggcgg gatccaacgc aacggcctgc cgtaacagcg
    15781 ccacgctctt cgatggcggg atcttcgagt ccgcatatac cgcgaagtgc agcagcctca
    15841 aaacctcgat ccggtccttc ggatcgactc ctttgccggt cagcgtgaac tcccgctgcg
    15901 aggacccgcc cagatatccg agcgacctca actgctcgag ggtgcgccgg tccgaggctc
    15961 cactctcgac cttctccacc tccgggctca acgttttgag ctgtgcttcc agctccctcg
    16021 ctttctccgc gtgagccgca atcacattcg tgctctcgcc ggggtcgtgc acgagatcgt
    16081 acagctcggg ccgtggcgca cggatgtact tccactggtt cgttctgatt ccgcgcagct
    16141 ccgtccaccc catgttgatt ttcggaaaga gggtttccac atatgcatat gccgccggcg
    16201 cctcttcgct gttgaaggca ggcacaaggc tggcgccctg cacttccgcg ggtgccttgc
    16261 cgcccatcag ctcgagcacc gtcggcagca ggtcgatgct gcgcgcctgc tgttttttcc
    16321 gcacacccgg cggaactccc ggtcccgcta gcagaaatgg aatccgcagc gtggtgtcgt
    16381 acagaaaaat gccatgcgca tactcgccgt gctctgaaaa actctcgccg tgatcggaaa
    16441 gcaccgcgat cagagtgttg cccgaagact tgctttcgat gcggtcgagc aaccgcccca
    16501 gttcccggtc ggtgtaagcc acctcgccgt cataaggcct tccacggtat tgttcccgga
    16561 agggagaagg cgggtcataa ggcagatgtg gatcgaacac gtgcacccag agaaaatacg
    16621 gttttcccga ttgcgcatcg agccattgaa tcgcacgccg gacgacctct tccgcacgac
    16681 gctccggcgc ttccgatgct gcgccgccgg cgtccatctc gtcgtcgtag accgcgaacc
    16741 cctggttgaa tccgaatcgc tgtttcagca cggatgcgcc cacgaacgcg gccgtgtccc
    16801 agccctgcgc ttgtagtacc tccgccagcg tcgtgtgctg cgacggcagg atgaagccgc
    16861 cggtatcccg cactttgtgt gccgtcggat acaaaccggt gaacatgctc gcgtgcgacg
    16921 gcgccgtcag cggagcctgg gcaatggcgt tttcgaacag gactccccgc tgcgccagct
    16981 tgtccagatg cggcgtttcg gcattcgcgc cgtagcagcc gagccggtcc gcgcggagcg
    17041 tgtcaatggt caccagaacc aggttcatcc ggcgaagcgg cgccgcctct tccgtccgct
    17101 tctggtttgc agaccggcag gaagccagca ccagcagagc cgtggcgcat acgcccccat
    17161 agaccagcaa tcgaatcggg agtgccattc tcctgctcac gagaccacgg tacactaccg
    17221 cccaaccagg ccgcagccgc cgaagtccgc gagcggaatc aaggcaatcg aaaccctacg
    17281 cttcccgcga cgacagggtt gtaattccac cggttccagt aagtaaacga ggaaacaaat
    17341 ccggtggccg gggtccggta gaggtagtta cgcagttctc cacgcaggaa ccagcggcgg
    17401 gcaactctta tgtcggcgcc tgcccccggt gtgagcacta cggtgagatt tgagtcgctt
    17461 tgcgaggctg tttgctggcc actctgaaag tcagtgcccg tgcggcgaat tgtcccgagg
    17521 ccggccccga acgatagcca gggggaaaga cgccgcccgg ggccaaatcg gacccgaaag
    17581 ccgggcgtga gggcggcggt cagccgctca gtgtagatgc tggcatagaa tccgctggca
    17641 aagacatccg aactgcggga tccatggagc gcgaccggaa cgtcaatccc aatcgaaaca
    17701 ctcttgtggt gcagaaactc gagtccggcg ttcaacagga gagttccgcc ggtttccggt
    17761 ttcgcatccc gtccggccgg gtccgtgcgg gcattgcccg aggcgggacc cgcacctccg
    17821 ccgattgcga cggacagatg ctggcaaatg gcgcttggcg ccaggcaaag aaagatgatt
    17881 atgcaacagg tattcaggct catattggct tagaaagcaa agaccggtga accaccgctt
    17941 tcaactacac gagcctgggc cggaacgccg gaaaccggct ccgcacgtgc agcgccaatg
    18001 ctacgagtga acgttctgct agcgattctc tcccggcagg tccaacgatt ccggatcagg
    18061 ctcctgcaga tctatgcagt tcaactccca atgcgataac tgagtgcggg tttcgaccat
    18121 atcgctcacg acgatggcga gttcctcaca gatccacact tcgtttgtga ttaagtttgg
    18181 atcttcctcc ggtagccata gttggatccg gcggcactcc tggccgtgaa tcaggcggct
    18241 ttcatcagtt cgcttcatat gtacaagcgg gccggttcca cctcttggga gccacacttc
    18301 ctctatgctg gggggccata cactctgctt gccctcaaca tatacttcca tcttccgatc
    18361 cgaggggttc cagcgttgcg cgaaagttac acctttttcg ggcgcgttcg gctgcagcag
    18421 ctcgatcttg gtgatgcctg cggagtttcg gtactggcgg ccttgtgcac acgccagccg
    18481 gggttcctta agcttttcga cgaaaaccgt atgaaaggcc gcgatgaacg gacgatccaa
    18541 gaccgccgtc gtggggggaa ctaatcgcgg aagtaccaga aatccctcgt ttgcgttagc
    18601 cacggcctgt ctctccaaac actataggat aggacagagt gatcaattta ggcgactcca
    18661 ttcacacgac ctaagaccca tctcgaaagt gcaaaatctg gtcgcagcag ctaccagctt
    18721 aaggaatcac tgacacggac ctggtgaagg agatgtgaag tctggtccac ccattttgaa
    18781 gcactgcaaa ctggctccag caatttgggc ggacaggagc cgccacgtcg catatggcat
    18841 gggacacgga gtctgccagc cagtgatata ggtgaggttg ggaccacaag acggtgtcat
    18901 gcccccgatg caccctggga cgtaggagca caagttgtag acaccgggca caacctgaga
    18961 accagtattc cggcccgtgt ataagcggta tccgatccgg atttccgacg agacagaggt
    19021 tcctaagaac cccccacacc cggttctatg tgaactagta gccgaatagg ttccctcaac
    19081 cgcaatgagg ttgacggata cgctgtgata tccagatcct acgccttcgg caacgggggt
    19141 tccgtttggt ccgtagagag ttgtttgcac tatcgcctca tgttggaccc cgtggtgcaa
    19201 gtcgtccgtt accccgactg tccgtatcct aactgtgtcg ccgtcactca attgttcagc
    19261 ccatatgtca gtgtagacaa gggtaccctg gctgaaggca aagtcaagct ttttctgtaa
    19321 attcattctt cccgcgtaat cgggccactg ttttccattc gtaagtagat cttttcggtt
    19381 tcccactcct cgctgatttc catcagtacg gcgctgacca ggcgcaacag cgcagc
//
LOCUS       DASTMK010000174         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_104830, whole genome shotgun sequence.
ACCESSION   DASTMK010000174 DASTMK010000000
VERSION     DASTMK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104830"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFQ79_07460"
     CDS             complement(<1..779)
                     /locus_tag="VFQ79_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0139529.1"
                     /translation="MHSVCYPCRQNGPALPNGRADLILIECRGSEQDCGFELFKCLRT
                     VDAVTPVILVVRGGSEALAIEALRLGAHDYLKMPVEARDLVTAVKHCLGACPTGSDRE
                     TPGLIGGSEAMQNVRESIRRLAPTDTTLLITGETGTGKELVAQMLHRMSRRRNKPLVC
                     INCAAIPDTLLESELFGYERGAFTGAVAAKPGKLKMADGGTVFFDEVGDMSPFAQAKI
                     LRAIEAKEVHSLGSSHGTRVDIRIICATNRDLEKLMGEDKFR"
     gene            1030..2145
                     /locus_tag="VFQ79_07465"
     CDS             1030..2145
                     /locus_tag="VFQ79_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU0139530.1"
                     /translation="MSGVRVLVGTRKGAFLLTSDGKREQWDISGPHFAGWEIYHVKGS
                     PADPNRLYASQSSGWFGQMIQRSNNGGKTWEAVGNQFTYDGVPGTHQWYDGTPHPWEF
                     KRVWHLEPSLMDPDTVYAGVEDAALFRSTDGGQSWQELSGLRGHGSGPHWMPGAGGMC
                     LHTILLDPEDSRRMFIAISAAGTFRSDDGGTTWRPINRGLKSEHIPDPDAEVGHCVHR
                     IAMHRSRPNVLFMQKHWDIMRSDDAGESWREVSGNLPTDFGFPIDVHAHEPETIYVVP
                     IKSDSEHYPPEGKLRVYRSRTGGNEWEALTRGLPQSDCYVNILRDAMAVDSLDSCGVY
                     FGTTGGQVYASADAGDNWAPIVRDLPPVLSVEVQTLS"
BASE COUNT          419 a          662 c          692 g          433 t
ORIGIN      
        1 cggaatttgt cttcgcccat gagcttttcg aggtcccggt tcgtcgcgca aatgatgcga
       61 atgtccaccc gggtaccgtg cgagcttccg aggctgtgga cctccttcgc ttcgatagcg
      121 cggagaatct ttgcctgggc aaaaggactc atatcgccga cctcgtcgaa gaaaaccgta
      181 ccgccatcgg ccatcttcaa cttgccgggt ttcgccgcga ccgcgccggt gaaggcgccg
      241 cgttcgtatc caaacagctc gctttcgagc agcgtgtccg gaatggccgc gcagttgatg
      301 cacaccagcg gcttgttccg gcggcggctc atccggtgca gcatctgagc gacgagttct
      361 ttgccggtgc ccgtttctcc ggtgatcaga agcgtcgtat ccgtgggcgc cagccgccgt
      421 atggattcgc ggacgttttg catcgcctcg cttccgccaa tcaagccggg cgtctcccga
      481 tcacttcccg ttgggcaggc gccaaggcaa tgttttaccg cggtgacgag atcgcgggcc
      541 tcgaccggca ttttcagata atcatgcgcg cccaggcgca acgcttcaat ggccagcgcc
      601 tcggaccccc cgcgtaccac taaaatgacc ggcgtaaccg cgtccacggt ccggaggcat
      661 ttgaacagtt cgaacccgca atcctgctcc gaaccgcggc attcgatcag aatcaggtct
      721 gcccgcccgt ttggcaacgc cgggccgttc tggcggcatg gataacagac gctgtgcaac
      781 ccgctatctc gtagcaaaga ctggagttca gagccgagag gaggttcctg cgtaaaaatt
      841 gctatgacgg gcgtcacggg tttttgaatc cagtttgctg ctttgcctat actgaatcag
      901 cctggtacca cgtttcaagg gctccagtac ctgggtgggg ttgtggatcc aaataatttg
      961 tgccgggttg tcgatcctgg aaagggccgt tcgatccatt gccagaggga cttaaaaagg
     1021 gataacaaga tgagcggtgt acgagtactg gttggtacgc gcaaaggtgc ctttctcctg
     1081 acatcggatg gaaagcgcga acaatgggac atcagcggtc cgcattttgc gggatgggag
     1141 atctaccacg taaagggatc ccccgcggac ccgaatcgcc tgtacgcttc ccaatcgagc
     1201 ggctggttcg gacagatgat ccagcgttcc aacaacggcg gcaagacgtg ggaggcggtg
     1261 ggaaaccagt tcacctacga tggcgtgccg ggcactcacc agtggtacga cggcacgccg
     1321 cacccctggg agttcaagcg ggtctggcat cttgaaccat cgttgatgga cccggacacc
     1381 gtgtatgccg gggtggaaga cgccgccctg ttccgttcga cggacggcgg gcagagctgg
     1441 caggagcttt cgggattgcg cggccacggt tcgggtcccc attggatgcc gggcgccggc
     1501 gggatgtgcc tgcataccat ccttctcgac ccggaggata gccggcggat gttcatcgcc
     1561 atttcggcgg cgggcacctt ccggagcgac gatggcggca caacctggcg gccgattaac
     1621 cgcgggctga agtcggaaca catccccgac ccggacgccg aagtcgggca ctgcgttcac
     1681 cgcatcgcga tgcaccggtc gcgtcccaac gtcctgttca tgcagaagca ctgggatatc
     1741 atgcgcagcg acgatgcggg cgagtcctgg cgcgaggtga gcggaaatct gccgacggac
     1801 ttcggatttc cgatcgacgt tcacgcgcac gagccggaaa cgatctacgt ggttccgatc
     1861 aagagtgact ccgagcacta tccgcccgag ggcaagctgc gcgtgtaccg cagccggacg
     1921 ggtggaaacg aatgggaggc gctcacccgg ggacttccgc aaagcgactg ctacgtcaac
     1981 atactgcgcg atgccatggc ggtggattcg ctcgattcct gcggcgtgta cttcggcacc
     2041 accggcgggc aggtatatgc ttcggccgac gccggcgaca attgggctcc cattgtccgg
     2101 gaccttccgc cggttctttc cgtcgaagtc cagacgctgt catgatccgc gtagtgcttc
     2161 cggcgcacct gcgcacgctg gcgcgggtgg aaggcgaggt aaaggt
//
LOCUS       DASTMK010000175        24061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_136517, whole genome shotgun sequence.
ACCESSION   DASTMK010000175 DASTMK010000000
VERSION     DASTMK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24061
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136517"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFQ79_07470"
     CDS             complement(<1..683)
                     /locus_tag="VFQ79_07470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0139531.1"
                     /translation="MEAGKPRFLANSLLHVTVVMPLRDDWLSAGELIRHLDRTIPSSL
                     CHLEILLVDDGSTEACRPADFQHDFMSVKIIRTLRLRRNLGHQRAIAIGLAHIERTAS
                     CDAVLIMDADGEDTPDGALQLITEFVAGEGVHAIFAERSRRTEPIVFRFLYAVYKILH
                     RALTGLSVRVGNFSILPAKYLSTLVVVPEMWNHYAAAVFRSNLPFTMIPIPRGYRIAG
                     TSRMNFVSLV"
     gene            complement(792..2336)
                     /locus_tag="VFQ79_07475"
     CDS             complement(792..2336)
                     /locus_tag="VFQ79_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139532.1"
                     /translation="MRRIVIIITAVLSLIALCGLISAQNTVFPHRWVRISSQLRTDED
                     VEKIRRLARVASENGLTGVLLAIGLDSIDLKGPDYMTRLEAVKDILKQNRLEMIPNIF
                     SGGYGGAILAHDRNLAEGFEVRDLLYTAKEGQAHLVPEVVLDYNGPAVEQMWTREVTV
                     KPYRCYRVTFRAKTEGLPQTRAFTTGSFRLTVQTADGRNLTPWNARIPSSTDWREARW
                     GFNTQGYDKVTISIGIRDNTPGNAWVDRVHVEEVGLLNVLRRPGTPVVVRADESDTVY
                     EEGRDYDRIVDPQLNFRFDHDGPPIRLLPGSRIREGQKLRVSYYHGFSINDGQTTICM
                     GEPKTYEIWRDMARRMHAAAAPPRYVLSMDEMRSGGTCAACRGRDIAQLFGECVTRQF
                     QMLREVNPKVDVWIWSDMLDPNHNARDKYYLVNGDYTGSWNHVPRELGIVAWYYQRRA
                     LSLPFFSKLGFRTIAGAYYDADTLDNPRGWLEELRKTPNAQGIMYTTWLNKYELLAEF
                     GQLVSK"
     gene            complement(2368..3099)
                     /locus_tag="VFQ79_07480"
     CDS             complement(2368..3099)
                     /locus_tag="VFQ79_07480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0139533.1"
                     /translation="MLPKLYDQLSSWWALLSPPSDYADEAEFYARELIAAGSEPAHTL
                     LELGSGGGNNASYMKKHFHMTLVDLSPGMLSVSRDLNPECEHIQGDMRAVRLERQFDR
                     VFIHDAIVYMTTISDLRKAIETAFLHCRPGGAALFAPDHVQETFRSYTDHGGCDGEDR
                     GLRYLEWTWDPDPTDSTYLVDFAYLLRDKDGDVRVEHDRHVEGLFGRNEWLQLLTEAG
                     FQPRNMPLAIPDVEPGTYEVFLGVK"
     gene            complement(3129..4184)
                     /locus_tag="VFQ79_07485"
     CDS             complement(3129..4184)
                     /locus_tag="VFQ79_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU0139534.1"
                     /translation="MSRIALWITPFLLLFAQTDALLPSRDAVKLCERVTQLMEATGVA
                     IPELARAGAPVAENAKQALTNLRSSPGMSHAGYAYTFLSNVKAFLVLSDAVPKPFPLQ
                     ETTGKQLVELREASGRIEAHFRALLEQKERQLRNPDRDNLMRYAEANGNLPAPQANSR
                     VVFLGDSITDGWRLNQYFQGRDFVNRGISGQITGEMLGRFKADVIDLKPAAVLILAGT
                     NDLARGVRVNVIQNNLSMMVDLALANNIKPILASVLPIHDYNKGVNPQFEQSPRRSPE
                     LIRTLNTWMERYASDKHIAYLDYYSAMVDASGFLQRELADDGLHPNSAGYRVMAPLAL
                     AAIEKTLAPAPAGKRRR"
     gene            complement(4181..4987)
                     /locus_tag="VFQ79_07490"
     CDS             complement(4181..4987)
                     /locus_tag="VFQ79_07490"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU0139535.1"
                     /translation="MLCRVFSRRIAAPLVLALCLPTYSFQAKPERAESSVDRQKISNG
                     GETLHYSVEWRLIEAGRARLSWTPTKTGGEPGWQVDLQLESTGLVSKLYKVEDHYRVM
                     LTESLCATSSLLKANEGRRQRETKVTYDRINKKAYYLEWDLIKDAAVRQNEVAIPDCV
                     HDVVGALAALRAKDFDPPASMQFPISDGKKFVQAKVEAQEKETIQTPAGSYKTIRYEA
                     FLYNGVLYSKKGRLFVWLTDDERRMPVQIRIRLGFTVGTVTLQLEKEESA"
     gene            complement(5039..5941)
                     /locus_tag="VFQ79_07495"
     CDS             complement(5039..5941)
                     /locus_tag="VFQ79_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139536.1"
                     /translation="MRRREFLLSCAAAGAAPDSILVHEHVMVDFIGAEKISPGRYDAD
                     EVFRTAKPKLEEVKRLGCRRLQECTPAYIGRDAVLLARLQEATGVEIWTNIGIYGAAN
                     HKFVPDYARRESAEQLASRWIAEWHNGIEGVKPRFIKTGVNHGPLAELDRKLVRAAAI
                     TSRETGLTIASHTGNGIAAMEQAEIVLAEKVPASRFVWVHAQSEKDHSFHEKMAREGF
                     WVEFDGIGPKSADWHLECVRYMAERNLLSRTLISQDAGWYHVGEPGGGNYRGYTYLYT
                     DFLPRLKDSWRRTLMWDNPRMAFG"
     gene            complement(5941..7518)
                     /locus_tag="VFQ79_07500"
     CDS             complement(5941..7518)
                     /locus_tag="VFQ79_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU0139537.1"
                     /translation="MFEASRLLIPLIVLFVLLALFIATALFSRNYLKVPPNTVAVLSG
                     RKRKLAEGRTVGYRMVRGGAALRIPLLEKVDYLSLNVLTIPLEIKRAYTLKGVPVSVK
                     AVANVKIRSDETSLHAAAERFLGMTHDNIQRVIFQTLEGHLRSILGTLTVEEVNSDRQ
                     SFAQKLTSEAAADLEKMGLGVDVLTIQEVSDEEEYLNALGKRRTAEVKRDAVIGEADA
                     TRDAKIRSAHALQEGERAKLNADAEIAQAQRDLQIRQAQYQAEVEREKASADQSGPLA
                     SAKARQAVIAEEVRVERTRTQEMISVQQQEVLRKQQELEATVIKPADAERQAAVVRAE
                     GAKTAAILEAEGRRAALIAMAEAEQEKLRKEGAGRASALETEGRAEAAKIEAIGLAQA
                     KAIEAQGIAEAAAILRKAEAWKQFNEAARLQTILEKLPAILQASTGIFSSVAAPLGNI
                     DKLVVIDQGNGASEANSGISRLAKAGPAVVFGLLQQLEALGLNVPAVLNQLGISESAD
                     GRKATAAGVGDGAVKVD"
     gene            complement(7531..8070)
                     /locus_tag="VFQ79_07505"
     CDS             complement(7531..8070)
                     /locus_tag="VFQ79_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139538.1"
                     /translation="MAEVISQLSAFSIFLGIAAVGFLFLLVSLVFGEVFDHLGSDFDH
                     DLDHGPSFFSPRILSVFVTAFGGTGAVASHYGVGVVHASGAGFVSGVVFASIIFLFAR
                     FLYGQQASTDVRQIDVVGQNARVIVSIPSGGVGQVRCRVGEELVDKVAKSFDGGAIPE
                     NRIVKVEEALGEIVIVRPQ"
     gene            8143..10011
                     /locus_tag="VFQ79_07510"
     CDS             8143..10011
                     /locus_tag="VFQ79_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0139539.1"
                     /translation="MTLATRFTYILLLLVPAAFAQAPEGSYSVEKNVMVAMRDGVRLA
                     TDLYFPARGGVRLEGKFPAVLERIPYNKDGAGKGWAPWFAARGYVAVVQDTRGRYGSE
                     GVWHMMTDDVPDGFDTASWIVKQPWSDGGFGTVGTSYAGGTQHALALSNPPGLKAMVP
                     VDAVANAGYFGMRNGGAFELRFMNWIFTIGAPRGSRASRDPGTQAVLDEAGKNVRQYL
                     DNLPLRKGLTPLRLAPEYEDWLVEAMARGENDSYWKQPGFNVVDQVPAYKDIPVYLIG
                     GWYDSWARQTTMTYEALHKAKQSSIKMILGPWIHGAHNNHAHGQVDFGSDAAINGLEF
                     RARWFDRWLKSTTNGVESDAPVKIFVMGGGTEAKNAKGEHEHGGVWRDEKEWPLARTR
                     FTPYYLQAGGGLTTAKPTARNASAAYDFDPRNPVPTIGGNISSGVGIMLQGGWDQKCG
                     EHVWNCKDTLPLSARRDILVFQTPPLTEDVEVTGPIDVKLWASSSAPDTDFTAKLIDV
                     HPPSPDFPGGIDMNIEDGILRARFRDSLEKQELMKPGTVYEFTIKLYPTSNVFKAGHR
                     IRLDISSSNYPRFDVNPNTGEPLNQHRRMVTATNTVYFDAARPSHVILPVIPASAR"
     gene            10031..11098
                     /locus_tag="VFQ79_07515"
     CDS             10031..11098
                     /locus_tag="VFQ79_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139540.1"
                     /translation="MRRIFALLGLFALPVMAQEYKIAVVGLVHSHVWGHLGKMVSGQP
                     AKLVGIAETVPELVAEAKKRGATDIPFFDDYKKMLDDAKPDIVWAFVENNRHLEIVQA
                     CAPRKISVMFEKPLASTYKDAVAIQRLAKQHGIQVMTNYQMAWWPSNYTARKAAESGA
                     VGPVYRLHGIVGHGGPGGPTGLNKYFFAWLTDPVKNGAGALMDFGCYNALWSLWYLGK
                     PQTVYATVQQLRPQEFPKVEDNSTMILSYKDAVGIFEGSWDLPRSFQDLEVFGRSGSL
                     YVTGKGVEHRKGRSEPAQLPAVDLTPAESEPIAFMIDALRNKKPIEGLTALDINVDVV
                     EIIEAAKMSVKSGKAVVLPLK"
     gene            complement(11106..11801)
                     /locus_tag="VFQ79_07520"
     CDS             complement(11106..11801)
                     /locus_tag="VFQ79_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139541.1"
                     /translation="MSGNEQDFWGLNYFNYFTEVEEHFQKARGTGLFLLSPLDWALIE
                     AWKNAGIPLEAVLRGIDSAFEKWRSRKSRIQMVNSLAYCMQAVLTEAQVMAGAVPATK
                     KPVEAPFDIEELKDFLSMNVLDLRKRGAPAYEAVAKTLEKILEEADSHYKDLESLEQR
                     LTALEEKMIAIARAQQSDDDLLRARKELESELRPYRSKMTAEQLTMLEKQYLDRKLLE
                     SAGLPRLSLFYLR"
     gene            complement(11798..12568)
                     /locus_tag="VFQ79_07525"
     CDS             complement(11798..12568)
                     /locus_tag="VFQ79_07525"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU0139542.1"
                     /translation="MLLALDVGNTNVTVGAFQDDGLAARWRLRTVHEQTADEWGILLR
                     NLFAHSAVKLEAVDGIVISSVVPPVDSVLADMAGRYFDRRPVFVTPETDTGLKVMYDN
                     PKEVGADRIVNAVAAYDRLGGPCVVVDLGTAITFDAISNKAEYLGGLIYPGLGISIGA
                     LFHRTARLPKVDLREPARLIGTNSVASIQSGLYYGTIGTIDGILQRLIGALGPDTKVI
                     GTGGQARLISGGSRLLRDVDEDLTLKGLKLLWERNRKP"
     gene            complement(12569..13333)
                     /locus_tag="VFQ79_07530"
     CDS             complement(12569..13333)
                     /locus_tag="VFQ79_07530"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU0139543.1"
                     /translation="MPFDLEWVRSRLPGREIHWFDRIASTMPAAAQLASAGCTPGTIV
                     GADEQTAGQGRFGRKWQSERDAGLYFSVVLDVPSQPAEVPLLTLALGVAVARAIHDST
                     GLDCDLRWPNDVLIGGKKCAGVLTTLHEHRAVAGIGVNVNQTKFPAELAAIATSLEIA
                     SGREHSREVLLVSLAESIDAETQLLARGAKREILEAFSRASSFVRGRKVTVEDGASIQ
                     TGITDGLDPSGFLLLRKDGGGTSVILNGGVRPAPGA"
     gene            complement(13333..14169)
                     /gene="nadC"
                     /locus_tag="VFQ79_07535"
     CDS             complement(13333..14169)
                     /gene="nadC"
                     /locus_tag="VFQ79_07535"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU0139544.1"
                     /translation="MFDVTHPEVVEAVRRALAEDIGPGDVTSEACVPAERRASGRFIA
                     RGEIIVAGIELLPLIYEMRGGVEACELRKASGDSARDGEVLANVTGNARTLLACERVS
                     LNFLQRLSGVATLGRRYVEAVAGTKCRILDTRKTTPGLRWLEKMAAAAAGVTNHRMGL
                     FDAILIKNNHIAAAGGITPALERASRSGLPVEIEVRTRGEIEEALAAGAKHLLLDNMS
                     PEQAAEEIRHIGGRAAVELSGGINLESVRPYAEAGADFVSSGAITHSAAAVDINFRLE
                     LI"
     gene            complement(14174..16846)
                     /locus_tag="VFQ79_07540"
     CDS             complement(14174..16846)
                     /locus_tag="VFQ79_07540"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU0139545.1"
                     /translation="MQIEKVYEPQRFEPRWAQWWKESGIFHASPKAQGRVFSLVIPPP
                     NVTGSLHTGHMLEHSEIDMTVRWRRMLGYNTLWLPGTDHAGIATQMVVERRLAEEGLT
                     RHDLGREKFEKRVWEWKQEYGGTIRQQMIQLGASCDWSRERFTLDEGLSRAVRLVFVR
                     LFEKGLIYRGEYMVNWCPRCHTALSDLEVNHEETAGNLWHIRYPVKGTDRYLVVATTR
                     PETMLGDTAVAINPKDGRYTDLHGKTVQLPLMDREIPIILDSLADPEFGTGVVKVTPA
                     HDPNDFEAGRRHDLPKIKVIDEQARMTADAGPYAGLDRMEARKRVVEDLDKLGLLDKV
                     EPYTVAVGKCQRCKTAVEPLISTQWFVTMKPLAEPAIRAVEEGRIQFIPENWSKTYFE
                     WMYNIRDWCISRQLWWGHRVPAWHCRTCGRITVSADTPEKCAHCASDDIYQDPDVLDT
                     WFSSGLWPFSTLGWPEQTEDLAAYYPTSLLITGFDILFFWVARMIMMGIECTGEIPFR
                     QVYIHGLVRDAERQKMSKTRGNTIDPLEVSGRYGTDAVRISLLMGAAVGTDIVMTEDR
                     MISARQFANKIWNAARFIFLNMERSGVEPWIPSGPDSPRPEGSEINLAVPLEDRWIFS
                     RLNRCAEQVNRALEQYRYHEAAQTLWHFVWHEFCDWYIEIKKLRFQENSGLTADWRNI
                     LTVFESVLRLLHPAMPFLTEELWQRLASNAQEKPASIALARYPEHDAAISDHAAEREM
                     EILQEIVTSLRNLRADLKLDPKQQLDATVYSRTSALDVARSQKEAIERLANVKVTVSE
                     EHAPRGEGAVRSSAEFAVGLRVPVAQAEARRKRLVKDIEQLEKLIANSRRQLEDYEFL
                     SRAPEKVVQNIRQKLAEYEHQLEKNRADLDGLAA"
     gene            16927..17931
                     /locus_tag="VFQ79_07545"
     CDS             16927..17931
                     /locus_tag="VFQ79_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0139546.1"
                     /translation="MRLHFVLCLIGLATATVATPQQKKIVAVGLSPAAIEELNTVTPG
                     VRVVRVESDRLPGEVADADALFGTVNPRLIQAGKKLKWVQTYSAGVESYISPELQNSG
                     IVLTNAKIIQGPEIADHAMALLLALTRDLYLTIPKRTSQEWPRGEYRPVELNGKTAVV
                     IGVGGIGMQIAQRASAFGMEVIGVDPKGISYTTFLKEVVPPDRLDTVLPKADVVFVSA
                     PLTAKSEGMMGSKQFELMKKGSYFIAVSRGRLYDSAALVKALDSRQLAGAGLDVTNPE
                     PLPKGHPLWNFENVLITPHIAGQSDRIGERRMELLKENIRRFAKGETLINIVDKAEGY
                     "
     gene            18032..19150
                     /locus_tag="VFQ79_07550"
     CDS             18032..19150
                     /locus_tag="VFQ79_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEU0139547.1"
                     /translation="MEELRKHVEYRLTRQGRFLIAELLRPHNVLSTSVRNGGQQTSLR
                     YLVNHQSCEASDHRERQRLISEMGQEQYHNVACTEIGLDPQYVAMMGTAANMNYASIV
                     DMADDNLRVTAVVTAGVQGNAACAGDPAGWRETDEGWQKIAAAGTINTMLFVNHPVTE
                     GALARAAVTMTEAKSAALQRLAIRSLYSPDTATGTGTDQYCVAATIGGAKPLTSTSPH
                     VILGELIGRAVRDATLEALRWQNGLESSYTRGIFHALGQYGLNESSFFEDIASRLTDR
                     EMELLRRNNKAVFYEPMVGASAYAVASVLDRVRYQTLPPSAAREVIRQQAATLASNLA
                     AKPQAWAEFWSCLREADPAKPRELILDAIAAGWSSKWT"
     gene            19141..20115
                     /gene="cbiB"
                     /locus_tag="VFQ79_07555"
     CDS             19141..20115
                     /gene="cbiB"
                     /locus_tag="VFQ79_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEU0139548.1"
                     /translation="MDLAALHPNPQVLAGAVLLDLAIGDPVYRAHPVRLMGATLTWLE
                     NRLRAARLDGYGGGIALFLLLAVLWAGGIAAGLTALAQWSFWLCLAVHLFLVYSLLAL
                     HDLLRHAWAVERAARQHDLAATRVAVSRLVGRDTSRMDFAACRRAAIESLSENLADGF
                     VSPLFWYAIGGIPGLVVFKIVSTMDSMVGYKTERYLRFGWCGARLDDLMNLLPARMTW
                     LLISVVASFFPRCSALKALRIGWRYHSVLPGPNSGWSEAAIAGALQLRLIGPIWAQGL
                     LVTKVWVGDPADPPAGARSDVPRAAVFTALTGLLAAAAALAFIAAYPR"
     regulatory      20136..20339
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            20457..21035
                     /locus_tag="VFQ79_07560"
     CDS             20457..21035
                     /locus_tag="VFQ79_07560"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU0139549.1"
                     /translation="MSIATKRGDGGQTSLAGGIRVSKSCLRVEAYGTIDELNSVLGFA
                     RSICDDADLRERTRLIQRELFQVGSGLATPSVSRKPQIPVPAEIVDSLTAQVHEIEAT
                     EGLLSDWSLPGEHTAAAAYDVARTVCRRAERNVVRLMEAGETFDPNALAYLNRLSDLL
                     WLFGRKIELAAGVSGGLRAMNGKEGPRWSRAW"
     gene            21076..22071
                     /locus_tag="VFQ79_07565"
     CDS             21076..22071
                     /locus_tag="VFQ79_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0139550.1"
                     /translation="MIRIHRFSHALPLAVLVLAIGIIAVAVERFASSLGGLRITREGD
                     GYGRPEIRTESLKYPRAAVDSDLFRVRIAKPARRIVSQYWSIDEYLYSVVPPERVVAV
                     SQSAYERRISNVYPFAERFRPVIATDPEKVLRQTPDLILVSNSARLDFCDLVRTTGIP
                     IYRAFAMFTTLEQVAETIRLTGYLTGEDTAAAKEVDRFWSGIRRAQARKPANAPRPRI
                     LGMGGRSSYGDQTLFHDIVKTLGGINVGAEGGLRGYNSVNTEQIVQWNPQWIIAGADR
                     GKTKEVLDRMLQDPGIAVTQAARDGHIVVLENNVFLPASPFTTLLVNAIAEAIYG"
     gene            22064..23116
                     /locus_tag="VFQ79_07570"
     CDS             22064..23116
                     /locus_tag="VFQ79_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0139551.1"
                     /translation="MDKRRVRLELFYLVLLPVLALSGVIAIGIGSTEVSWRTILGVAA
                     QKLLPGGWVDTSGLTQADTVVVWLIRVPRVLVAAFVGAGLSAGGAVMQGLFRNPLAES
                     GLVGAGSGAALGSVIAFIAGWTVRSIVSLPVAAMIGALMALVVVYAMATRGGVTPVAT
                     LLLAGVAVTSLLTAVWSLLLSINIVNWQIAQEVLFWIMGGLDARSWTHVWLCAPFVTL
                     GIVAAIVQARELDLMQQGEETAAAFGVNVEHSKRMLILTASVLTGACVGVAGMVAFVG
                     LVVPHAVRLLIGPSHRPLLPACSIAGAAFLILCDLAARTIHPPTEIRLGVITSLVGGP
                     IFIALLMRRYREVGLA"
     gene            23113..23898
                     /locus_tag="VFQ79_07575"
     CDS             23113..23898
                     /locus_tag="VFQ79_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139552.1"
                     /translation="MSVLDATSISVSRGGRQILSDVTISLSSGEFLAIIGPNGSGKSS
                     LLRVLAGIWPAESGSVSLDGTPIGDMSRRDIARQVAFVPQDTRVDFAFAVEEIVAMGR
                     HPHRGRFTSERETDRRAINLALERCDVSSLRHRLVNTLSGGERQRVLIARSLAVEPAF
                     LLLDEPTANLDIQHALEILDLCRSLADGGQAIAFASHDINAVSRYASSVALMESGRIV
                     AAGTRNQVLTPRAVRDVFGVAIEVLAASNGQSLYLFHRRNNQI"
BASE COUNT         4705 a         7328 c         7046 g         4982 t
ORIGIN      
        1 acgagcgaaa caaaattcat ccgggatgtt ccggcgattc tgtaaccgcg cggaatcggg
       61 atcatggtaa acggcaggtt cgaacgaaac accgcagcgg cgtaatggtt ccacatttcc
      121 ggcaccacga cgagcgtgct aaggtacttc gccgggagaa tactgaaatt acctactcga
      181 acgctgaggc ccgtcaaagc gcgatggaga attttgtaca cagcgtacag gaatcgaaag
      241 acgatgggct ctgtccggcg gcttcgttca gcgaagatgg cgtgaacgcc ctctcccgca
      301 acgaattccg tgatgagctg taaggcgcca tccggtgtgt cctctccgtc ggcatccatg
      361 atcagcactg cgtcgcagga cgcagtccgt tcgatgtgtg caagcccaat tgcgatggct
      421 ctttgatggc cgagattgcg gcgcaggcgg agcgtccgga tgattttgac gctcatgaag
      481 tcatgctgga aatccgccgg acggcacgcc tcggtcgaac cgtcatccac aagcaggatc
      541 tcgagatggc acaaactcga ggggatcgtg cgatcaaggt gccggatcag ttcccccgca
      601 gagagccagt cgtcgcgcag aggcattact acggtcacat gcaacagaga attcgccagg
      661 aaacgaggct tgccggcttc caccactcac gatcaaggta tcatgccgtg cggtggtgct
      721 gagtgccggc actccgggcg cggcgacgcg gcagacggtg tggcgcgacg ccacaccgca
      781 ccgcttggag cttacttcga taccaactgc ccgaattcgg ccagcaactc atatttgttc
      841 agccaggtcg tatacatgat gccctgggcg ttgggcgtct ttctcaattc ttcgagccag
      901 ccgcgcgggt tatcgagcgt gtcggcgtcg tagtaggcgc cggctatcgt ccggaaaccc
      961 agcttggaga agaatggcag gctcagcgct cgccgttggt agtaccacgc cacgatgccg
     1021 agctcgcgcg ggacgtggtt ccaggaaccc gtgtaatcgc cattcaccag gtagtatttg
     1081 tcgcgcgcat tgtggttggg gtcgagcatg tcggaccaga tccagacgtc gaccttcggg
     1141 ttgacctcgc ggagcatctg gaactgccgg gtgacgcatt cgccgaagag ttgtgcgatg
     1201 tcgcgtcccc ggcaggcagc gcaggtaccg cctgagcgca tctcgtccat gcttagcacg
     1261 tagcggggcg gcgcagcggc ggcatgcatg cggcgggcca tgtcgcgcca gatttcgtaa
     1321 gtcttcggtt cgcccatgca gatggtggtc tggccatcgt tgatcgagaa gccgtgataa
     1381 tagctgaccc gcagtttctg tccctcgcgg atacggctgc cgggcaagag gcgaatgggc
     1441 gggccgtcgt gatcaaaacg aaagttcagt tgcggatcga caatccggtc gtagtcgcgg
     1501 ccttcctcgt aaacggtgtc gctttcgtcc gcgcgcacca cgacaggagt gcccggacgg
     1561 cggaggacgt tcagaagccc gacctcctcg acgtgcacgc gatcgaccca tgcattgccg
     1621 ggggtgttgt cgcggatgcc gatcgagata gttaccttgt cgtagccctg agtgttgaat
     1681 ccccagcgcg cctcgcgcca gtccgtgctc gagggaattc tggcattcca gggcgtgagg
     1741 ttgcggccgt ccgccgtctg gacggtgagc cggaacgacc cggtcgtgaa tgctcgtgtc
     1801 tggggaagcc cttcggtctt ggcgcggaag gtcacgcggt aacaacggta gggtttgaca
     1861 gttacttcac gggtccacat ctgctcgacc gccggtccgt tgtagtcgag cacgacctcc
     1921 ggaaccaggt gcgcctggcc ttccttggcg gtgtagagga gatcgcggac ctcgaagcct
     1981 tcagcgaggt tgcggtcgtg cgcgaggatg gcgccgccat atccgcccga gaagatgttc
     2041 gggatcatct cgaggcggtt ctgtttgaga atgtccttca cagcctccag ccgcgtcatg
     2101 tagtcaggcc ctttcaggtc gatcgaatcg agcccaatgg ccaggagaac gccagtgagg
     2161 ccgttctcgg atgcgacgcg cgcgagccgc cggatcttct ctacgtcctc gtcggtccgc
     2221 aactgagagg agatgcgcac ccaacgatgg ggaaacacag tgttctgagc ggagatcagt
     2281 ccgcacaggg caatgaggct gagtacagcc gtgatgatga tgacgatacg acgcatgcgt
     2341 aaattatatg gcggaaagcg gcgcctgcta cttcacaccc agaaatactt cgtatgtgcc
     2401 cggctcaaca tccggaatcg ccagcggcat gttacgcggc tgaaatccgg cttcggtgag
     2461 aagctgtaac cattcattgc gcccgaacaa gccctccacg tgacggtcgt gttcaacgcg
     2521 cacgtctcca tccttgtccc ggagcaggta cgcaaagtca accaaataag tgctgtcagt
     2581 ggggtccgga tcccaggtcc attccaggta ccgcagtccg cgatcctctc catcgcagcc
     2641 gccatggtca gtataagatc gaaacgtctc ctggacatga tccggagcga aaagggcggc
     2701 tcctcccggc cggcagtgca aaaacgcggt ctcgatagct tttcgcagat cggagatcgt
     2761 cgtcatgtac acgatggcat cgtggatgaa caccctgtcg aactggcgtt cgagccgaac
     2821 ggctctcatg tcgccctgaa tgtgctcgca ctccgggttt aagtcccggc tcacggacag
     2881 cataccggga gacaggtcga caagcgtcat gtggaaatgc ttcttcatgt acgacgcgtt
     2941 gttccctccg ccgcttccca gttcgagcaa cgtatgagca ggttcactcc cggccgcaat
     3001 aagctcgcgg gcgtaaaact ccgcctcgtc ggcatagtcg ctaggaggtg acaacagtgc
     3061 ccaccaggac gaaagctggt catacaactt cggcagcatt ggtatttccc tgctggaatt
     3121 aagcctgctt accgccgtct cttcccggct ggagcgggcg ccagcgtctt ctcgatcgcc
     3181 gccaatgcaa gcggcgccat cacccgatat ccggcgctgt tcggatgcag cccgtcgtcg
     3241 gccagctcgc gttgcagaaa tccggaggca tcgaccatcg ccgaatagta atcgagatac
     3301 gcaatgtgct tatcggaggc gtatcgctcc atccacgtat tcaatgtgcg aatcagctct
     3361 ggactccggc gcgggctctg ctcaaactgc gggttcacgc ccttgttgta atcgtggatc
     3421 ggcaacaccg aggccagtat cggtttgatg ttgttggcca gggccaggtc caccatcatc
     3481 gaaaggttgt tctggatcac attcacgcgt accccgcgcg ccaggtcgtt cgtccccgcg
     3541 agaatcagga ccgcggccgg tttcagatcg atgacgtcgg ctttgaagcg gcccagcatt
     3601 tcacccgtga tctgtccgct gatgccgcgg ttcacgaagt cccgcccctg aaaatactgg
     3661 ttcagccgcc agccgtcggt gatcgaatct ccgagaaaca cgactctcga attcgcctgc
     3721 ggcgccggca gattgccgtt cgcctcggca tagcgcatca ggttatcgcg gtcaggattt
     3781 cgcaactgcc tctctttctg ttcgagcaac gcccggaaat gcgcttcaat gcggccagag
     3841 gcctcgcgaa gctccaccaa ctgcttgccg gtggtctcct gaagcggaaa gggcttcggc
     3901 acggcgtcgg aaagcaccag gaaggctttc acgtttgaaa gaaaggtata ggcatagccg
     3961 gcgtgagaca tccccgggct gctccgcaag ttcgtgagcg cctgcttcgc gttttccgct
     4021 acgggcgcgc ctgcgcgtgc caactccggt atcgcgacgc ccgtggcctc catcaactgg
     4081 gtcacgcgtt cacaaagctt caccgcgtcg cgggagggca gcaacgcatc ggtctgtgcg
     4141 aacaacagga gaaacggcgt aatccatagt gcaattctgc tcaagcggac tcctcttttt
     4201 ccaattgcag cgtaacggta ccgacagtga atcccaggcg gatgcggatc tgcaccggca
     4261 tcctccgctc gtcatccgtc agccacacaa acagccttcc cttcttcgag tacaaaaccc
     4321 cgttgtagag aaaggcttca tagcgaatag tcttgtagga accggcgggc gtctggatcg
     4381 tttccttctc ctgcgcctcc accttcgcct ggacaaactt ctttccgtcg ctgatcggaa
     4441 actgcatcga ggccggcggg tcgaagtcct tggcccgtag cgcggcaagc gcaccgacga
     4501 cgtcatggac gcaatcggga atcgcgactt cgttttgtct tactgccgcg tccttgatca
     4561 ggtcccactc cagatagtag gccttcttgt tgatccggtc gtaggtgact ttggtctccc
     4621 gctgccgccg gccttcgttg gccttcaaca ggctgctggt ggcgcaaagg gattccgtca
     4681 acatcacgcg atagtggtct tccaccttgt acagttttga aacgaggccg gtcgattcca
     4741 gttgcagatc cacttgccag ccgggctcgc cgccggtctt cgtgggggtc cagctcaggc
     4801 gcgcgcgtcc tgcttcgatg agacgccact cgacggaata gtgcagcgtc tcgccgccgt
     4861 tggaaatttt ctggcggtca acggatgact ccgctcgctc cggcttcgct tgaaacgaat
     4921 aggtcggaag acacaacgca agcaccagcg gcgccgcgat gcgccttgaa aacacgcggc
     4981 aaagcatgaa acttgagttt ggcaaagccc cggttattcc gcaaccagcg ccccgtcctc
     5041 acccaaatgc catacgcggg ttgtcccaca tcagcgttcg ccgccaggag tctttgaggc
     5101 gcggcagaaa atccgtgtag agatacgtat agccccggta atttccgcct cccggctcgc
     5161 ccacgtgata ccagcccgca tcctgagaaa tcaaagtgcg cgacagcaga tttctttccg
     5221 ccatgtatcg aacacactcc aggtgccagt ccgctgactt gggaccgatg ccgtcgaact
     5281 ccacccagaa gccttcacga gccatctttt cgtggaacga gtggtccttc tcgctttggg
     5341 cgtgcaccca gacgaagcgc gatgccggga ccttctccgc aagcacgatc tccgcctgct
     5401 ccatcgccgc gatgccgttg cccgtatgag aggcgatggt cagcccggtc tcgcgcgatg
     5461 taatcgccgc agcgcgcacc agcttccggt cgagttccgc cagtggtccg tggttcactc
     5521 cggtcttgat gaatcgcggc tttacgcctt caatcccgtt gtgccattcg gcaatccagc
     5581 ggctcgcgag ttgctcggcg gattcccttc gcgcataatc gggaacaaac ttgtggtttg
     5641 cggcgccgta gattccgatg ttggtccaga tctcgacgcc ggtggcttct tgcaatctcg
     5701 ccagtaatac tgcatcacgg ccgatgtagg cgggcgtaca ttcctggagc cgcctgcacc
     5761 cgaggcgttt cacctcttcg agttttggct tcgccgtgcg aaagacctca tccgcatcgt
     5821 agcggccagg actgatcttc tcggctccga taaagtccac catcacgtgc tcatgcacga
     5881 gaatggaatc aggcgctgcg ccggccgccg cgcacgatag caggaattcc cgccggcgca
     5941 tcaatccacc ttcaccgccc cgtctccaac tccggcggca gtggctttcc tgccgtccgc
     6001 ggactccgaa atcccgagct gattcaatac cgctgggaca ttcaagccca gtgcttcgag
     6061 ctgctgaagt aatccaaata cgacagcggg accggccttt gccaagcgcg agatgccgct
     6121 gttcgcctcc gaggcgccat tgccctggtc gatgaccacc agtttgtcga tgttgccgag
     6181 cggcgccgcc accgaactga agatgccggt ggaagcttga agaatcgccg gcagtttctc
     6241 gagtatcgtc tgtaggcggg cggcttcgtt gaattgcttc cacgcttccg ctttgcgcag
     6301 aatcgcggcg gcttcggcaa taccttgcgc ctcgatggcc ttcgcctgcg ccagaccgat
     6361 cgcctcaatt ttcgccgctt cggcccgtcc ctcggtttcc agggccgatg cacgcccggc
     6421 gccctctttg cgcagctttt cctgttcggc ctcggccatc gcgatgaggg ccgcccgtct
     6481 tccttccgcc tcgagaatcg ccgctgtctt cgcaccctcc gcccgaacga cggcagcttg
     6541 cctctccgca tccgccggtt tgatgacggt ggcctccagt tcctgttgct tccggagcac
     6601 ttcctgctgc tgcaccgata tcatttcctg ggtgcgcgtg cgctccactc tcacttcttc
     6661 tgcaataacc gcctgtctgg ccttggcgct cgccaatggt cccgattgat cggccgaggc
     6721 cttttcgcgc tccacttcgg cttgatactg cgcctggcga atctggagat cgcgctgcgc
     6781 ctgtgcgatt tcggcatcgg cgtttagttt ggccctctcg ccttcctgca atgcgtgcgc
     6841 cgaccggatc ttcgcgtcgc gggttgcatc cgcctcgcca ataacagcgt cgcgcttcac
     6901 ttctgccgta cgccgcttgc cgagagcgtt cagatattcc tcttcatcgc tcacttcctg
     6961 aatggtgagg acatcgacgc cgagacccat cttctccaga tccgccgccg cttcgctggt
     7021 cagtttctgt gcaaagctct gccggtcgct gtttacctct tcaaccgtga gcgtgcccag
     7081 aatcgaccgc aggtgacctt ccaacgtttg aaaaatcact cgctggatgt tgtcatgtgt
     7141 catgccgagg aagcgttcgg cggcggcgtg aagcgacgtc tcatcgcttc gtatcttcac
     7201 gttcgccacc gctttcaccg acacgggaac acccttcagc gtgtaggcgc gcttgatttc
     7261 gagcgggatc gtaagaacat tcagggaaag atagtcgact ttctcgagca gcgggatgcg
     7321 caacgctgcg ccgccgcgga ccattcggta ccccacggtg cggccttcag cgagtttcct
     7381 tttgcgcccg ctcagcacgg cgaccgtatt gggcggcacc ttcaggtaat tgcggctgaa
     7441 aagagccgtg gcgataaaca gagccagcag tacgaacaag acaatcaatg gaattaaaag
     7501 ccgggatgct tcgaacatgg aatccttcct ttattgcggg cgaacgatga cgatttcgcc
     7561 cagcgcctct tctaccttga cgatgcggtt ctcaggaatt gcgccgccgt cgaacgactt
     7621 ggcgacctta tctacgagtt cctctccgac gcggcatcgc acctgtccga cgccgccgga
     7681 aggaatgctc acaatcacgc gcgcgttctg tccgacgaca tcgatttgcc gcacatccgt
     7741 tgaagcttgc tggccataca ggaacctggc gaacaggaag atgatggacg caaaaaccac
     7801 tccgctgacg aaaccggcgc ccgatgcgtg gacaacgccc acgccgtagt gggacgcgac
     7861 tgcgccggtc ccaccgaatg cggtaacaaa gacacttaaa attctggggc tgaaaaagct
     7921 cggaccgtgg tcgaggtcgt gatcgaaatc actgccaaga tgatcgaaca cttcaccgaa
     7981 taccagtgag accagtagaa acaaaaaccc aaccgctgcg atgccgagaa aaatcgaaaa
     8041 cgcagaaagc tgcgagatga cttcggccat aactggttct ccctgatagc aaaagtctat
     8101 aggagcgttt tactgaagtc aaaaccggat aagatcactg gaatgacact ggccacccgt
     8161 ttcacttaca tactgctgct gcttgtcccg gccgcgttcg ctcaagcgcc ggaaggttcc
     8221 tattcggtcg agaagaacgt aatggtggct atgcgcgatg gggtgcgcct ggcaaccgat
     8281 ctctactttc cggcgcgcgg cggcgtgcga ctggaaggca agtttcctgc cgtcctcgaa
     8341 cgcatcccct acaacaagga tggggccgga aaaggttggg caccctggtt cgcggcgcgc
     8401 ggctatgtgg ctgtggtgca ggacacgcgc ggccgctacg gctcggaagg cgtctggcac
     8461 atgatgacgg atgacgtgcc cgacggcttt gacaccgcct cgtggatcgt caagcagccg
     8521 tggagcgatg gcggcttcgg cacggtcggc acctcctacg ccggaggcac tcaacacgcg
     8581 ctggcgctat cgaacccgcc cggactcaaa gccatggtgc cggtggatgc ggtcgccaac
     8641 gcgggctatt tcggtatgcg caatggcggc gcattcgagc tgcgcttcat gaattggatc
     8701 tttacgatcg gcgcgccgcg aggcagtcgc gcttcgcggg atcccggaac ccaggcagtg
     8761 ctcgacgaag cgggaaagaa cgttcgtcaa tatctggaca acctgccgct gcggaaagga
     8821 ttgacaccgc tgcgcctggc gcccgaatac gaggactggc tggtggaggc gatggcccgc
     8881 ggcgaaaacg attcctattg gaagcagccg ggcttcaacg tagtggacca ggtgcccgcc
     8941 tacaaagaca tcccggttta cctcatcggc ggctggtacg actcctgggc gcgccagaca
     9001 acgatgacgt acgaagcact tcacaaagcc aagcaaagtt cgataaagat gattctcggc
     9061 ccctggattc atggggctca caataatcat gcgcacggcc aggtggactt cggatctgac
     9121 gccgccatca atgggctcga atttcgcgcg cgctggttcg accgctggct gaagagcact
     9181 acaaacggcg tggaaagcga cgctccagtg aagatattcg tcatgggtgg cggcacggaa
     9241 gcgaagaacg cgaaaggcga gcacgagcat ggtggtgtct ggcgggatga gaaggaatgg
     9301 ccgctggccc gcacccgttt cacgccgtat taccttcagg ccggcggcgg acttacgacg
     9361 gcaaagccca ctgcgcgtaa tgcttcagcg gcctacgact tcgacccgcg gaatcccgtg
     9421 cccaccatcg gcggcaatat ctcgtcgggc gtcggcatca tgcttcaggg tggctgggac
     9481 cagaaatgcg gcgagcacgt ctggaattgc aaagacacgc tgccgctttc ggcgcgccgc
     9541 gacatcctgg tattccaaac ccctccgctg acggaagacg tggaagtgac cggccccatc
     9601 gatgtcaagc tgtgggcgtc ctcatccgcg ccggatacgg atttcaccgc caagctgatt
     9661 gatgtgcatc caccgtcgcc cgattttccc ggcggcatcg acatgaatat cgaagatggc
     9721 attctccgcg cccgttttcg cgattcgctc gaaaagcagg aactgatgaa accgggcacc
     9781 gtctacgagt tcacgatcaa gctgtacccc acatcgaacg tcttcaaggc cgggcaccgc
     9841 atccgcctcg atatttcgag cagcaactat ccgcgcttcg acgtgaaccc caacaccggc
     9901 gaacccctga atcagcaccg ccggatggtg acggcaacca atacggtcta ctttgacgcc
     9961 gcgcggccgt cgcacgtgat tctgccggtg attccggcat ccgcgcgtta gcatgctaat
    10021 ttaaatgctt atgaggcgga tattcgcact tctaggtctc tttgcgcttc ctgtcatggc
    10081 gcaggaatac aagatcgccg tggtgggcct ggtgcactcg cacgtttggg ggcacctggg
    10141 aaagatggtt tccggccagc ccgcaaagct ggttggaatt gccgagaccg tccccgagct
    10201 cgtcgccgag gcgaagaaac ggggcgcaac agacattcct tttttcgacg actacaagaa
    10261 gatgctggac gacgcaaagc ctgacatcgt gtgggcgttc gtcgaaaaca accgccacct
    10321 ggagatcgta caggcgtgcg cgccgcgcaa aatcagcgtg atgtttgaga agccgctcgc
    10381 ttccacctac aaagatgcgg tggcaattca gcggttggcg aaacaacacg gcattcaggt
    10441 gatgacaaac taccagatgg catggtggcc gtccaactac accgcccgga aagccgccga
    10501 aagcggcgca gtgggcccgg tttacaggct gcacggcatt gtcggacacg gcggtcccgg
    10561 cggtcccacg ggcctcaaca agtacttctt tgcatggctg accgacccgg tgaaaaacgg
    10621 cgccggcgcg ctgatggact ttggctgcta caacgcatta tggagcttgt ggtacctggg
    10681 gaagccccag accgtctatg ctaccgtgca gcaactgcgt ccacaggagt ttccgaaagt
    10741 cgaggacaac tccacgatga ttctttccta caaggacgcg gtagggattt tcgaaggcag
    10801 ttgggatttg ccgcgcagtt tccaggacct tgaagtcttc ggccgttccg ggagcttgta
    10861 tgtcaccggc aaaggcgtgg agcaccggaa aggccgctcc gaaccggcgc aactgcccgc
    10921 cgttgatctc actccagcgg agtctgaacc gatcgcgttc atgatcgatg cgctccggaa
    10981 caaaaagcct attgaaggcc tcaccgcgct cgacatcaac gtggacgtcg tggagatcat
    11041 tgaagccgcg aagatgtctg ttaaatccgg aaaagcagtc gtgctgccgc tgaagtgata
    11101 tctccttacc ggaggtaaaa aagactcaac cgcggcaacc cagccgattc cagcagtttc
    11161 cggtccaggt actgcttctc cagcatcgtg agttgctccg ccgtcatctt gctgcgataa
    11221 gggcgaagtt cggattccag ttctttccgc gcccgcagca ggtcgtcgtc gctctgttgc
    11281 gccctcgcga tggcaatcat cttttcttca agcgccgtca agcgctgttc cagggattcg
    11341 agatctttgt agtgcgagtc tgcttcttcc agaatcttct ccagcgtctt ggcgacagcc
    11401 tcatacgcgg gagcgcctct cttcctgagg tcgagcacat tcatcgatag aaaatccttc
    11461 agctcctcga tatcgaacgg agcctcaacc ggtttcttcg ttgctggaac cgcgcccgcc
    11521 atcacctgcg cttcggtcag aacggcctgc atacagtagg cgagcgaatt caccatctga
    11581 attcgtgact tgcgggaccg ccatttttca aaggccgagt cgatgccgcg cagcacggct
    11641 tcgagcggaa tccccgcgtt cttccacgcc tcgatgagcg cccaatcgag cggcgatagc
    11701 agaaataacc cggtgccgcg cgctttctgg aaatgctctt cgacctcggt gaaatagttg
    11761 aagtagttga gaccccagaa atcctgctcg ttgccgctca cggtttgcgg ttccgctccc
    11821 agaggagttt caaacctttg agagtcaggt cttcgtccac atccctcagc agcctggaac
    11881 cgccggagat gagacgcgcc tggccaccgg tcccgatcac ttttgtatca ggccccagag
    11941 cgccgatgag ccgctgaaga ataccgtcga tggttccgat ggttccgtaa tacaagccgg
    12001 attggatgct cgccacggaa ttggtgccga tcagacgtgc cggttcacgc agatctactt
    12061 tcggaagcct tgcggtgcgg tggaacaatg cgccgatcga aataccaagc ccgggataga
    12121 tgaggccgcc gagatactcg gctttgttcg aaatcgcatc gaaggtaatc gcggtgccca
    12181 gatcgactac gacgcacggg ccgcccaacc ggtcgtaagc ggccaccgcg ttgacgatgc
    12241 ggtccgctcc cacctctttc gggttgtcgt acatcacctt caacccggta tcggtttccg
    12301 gcgtgacgaa caccggccgg cggtcgaagt agcggcccgc catgtccgcc agaacagaat
    12361 cgacgggcgg cactacgctg gaaatcacga tgccgtccac tgcctccagc ttcaccgcgg
    12421 aatgagcaaa caggttgcgc agcagaatgc cccactcgtc cgcggtttgc tcgtggacag
    12481 tgcgcagacg ccaccgcgcg gccagtccat catcctgaaa agctccaacg gtaacgttcg
    12541 tgttcccgac gtccaatgcc agcagcattt atgcccccgg ggcgggtctg acgccgccat
    12601 ttaaaatcac acttgttccg ccgccatctt tacgcagtag caggaaaccc gatgggtcga
    12661 gcccgtcggt gatgcccgtc tgtatcgaag cgccatcctc cacggtgact ttccttccac
    12721 gaacgaaact ggaagcccgg ctgaacgctt cgagtatctc ccgctttgct ccgcgcgcca
    12781 gcaactgcgt ttcggcatcg atggactcgg ccaggctcac caacagaacc tcgcgggaat
    12841 gctcgcggcc ggaagctatt tcaagcgaag tggcgatggc cgccaattca gccggaaact
    12901 ttgtctggtt gacgttgacg ccaatgcctg caaccgcccg gtgctcatga agagtggtaa
    12961 gaacaccggc acattttttt ccgccgatca gaacgtcgtt cggccagcgc aagtcgcaat
    13021 caagccccgt ggaatcgtgg atagcacggg ccacagccac acccagagca agcgtgagaa
    13081 gcggaacctc cgcgggttgc gaaggaacgt ccagcacgac ggaaaaatag agcccggcgt
    13141 cccgttccga ttgccacttt ctgccgaagc ggccctggcc cgccgtctgt tcatccgcgc
    13201 caacgatggt gcccggcgta cagccggccg aggcaagctg cgccgcagcg ggcattgtgg
    13261 atgcgattct gtcaaaccag tggatctccc ggccgggcag ccgggaacga acccattcca
    13321 ggtcgaaagg cattagatca gttccagcct gaagttgatg tccacggctg cagccgaatg
    13381 tgtgatggcg ccggaggaga cgaaatccgc gccggcttcg gcgtaaggac gaacgctttc
    13441 aaggttgatg ccgccggata gctcaaccgc agcccttccg ccaatatggc ggatttcctc
    13501 cgccgcctgc tccggagaca tgttgtccag cagcaggtgc tttgcgccgg ccgccagagc
    13561 ctcttcaatc tcaccccttg tgcggacttc gatttcaacc ggcaacccgg agcggctcgc
    13621 gcgctccagc gccggggtga ttccgcccgc cgccgcaata tggttgttct tgatgagaat
    13681 ggcatcgaac aggcccatgc gatggttggt gacaccggcg gcagccgctg ccattttttc
    13741 caaccacctc aggccgggcg tggtcttgcg cgtatccagg atccggcact tcgtacccgc
    13801 caccgcttcc acataccttc gcccgagcgt ggccacaccg ctgaggcgtt gcaggaaatt
    13861 cagagaaacc cgctcacatg cgagcagcgt tcgggcattt ccggtcacgt tcgcgagcac
    13921 ctctccgtcg cgcgcggagt ccccgcttgc tttcctgagc tcacatgcct cgacgccgcc
    13981 gcgcatttca taaatcagcg gcagaagctc gattccagca acaatgatct cgccccgcgc
    14041 aatgaagcgg ccggatgccc gccgctccgc cggcacgcac gcttccgagg tgacatcccc
    14101 cgggccgata tcttccgcca gcgcgcggcg gacagcttcc actacttccg gatgcgttac
    14161 atcgaacatt gttctatgcc gccagcccgt ccaggtccgc gcgatttttc tcgagttggt
    14221 gctcgtattc ggccagcttc tgccggatat tttgcaccac cttttccgga gcacggctga
    14281 ggaattcgta gtcttcgagt tgccgtctgg agttggcaat cagcttttcg agttgctcta
    14341 tgtctttcac gaggcgctta cgccgggctt cggcttgcgc caccggaacg cgcaggccga
    14401 ctgcgaactc ggcggacgag cgcacggcgc cctcgcccct cggcgcgtgc tcttcagaga
    14461 ccgtaacttt cacgttcgca agcctctcga tcgcctcttt ctggcttcgc gccacatcga
    14521 gagcacttgt cctcgaatac acggttgcat cgagctgctg cttcgggtcc agtttcaggt
    14581 ccgctcgcag gtttcgcagc gaggttacga tctcctgcaa aatctccatc tcccgctccg
    14641 cggcatgatc gctgatcgcg gcatcgtgct cggggtaacg ggcaagcgca atggaggcgg
    14701 gcttctcttg cgcattcgaa gcaagccgct gccacagctc ctccgtcagg aatggcatcg
    14761 ccggatgcag cagccggaga acgctctcga acaccgtcag gatattgcgc caatcggcgg
    14821 tcaaccccga gttctcctga aaccgaagct tcttgatttc gatgtaccag tcgcagaatt
    14881 cgtgccagac aaagtgccac aacgtctgcg ccgcctcgtg atagcgatac tgttcgagcg
    14941 cgcgattcac ctgttcggcg caacggttca ggcggctgaa gatccagcgg tcctcgagcg
    15001 gaacggccag gttgatctcg gacccttccg ggcgcgggct atcgggcccc gagggaatcc
    15061 acggctccac gccggatcgt tccatgttga ggaagataaa tcgtgcggcg ttccagatct
    15121 tgttggcaaa ctggcgcgca ctgatcatcc ggtcttccgt catgacgatg tcggttccca
    15181 ccgcggctcc catcaggagc gatatgcgaa ccgcatccgt tccgtaacgg cccgacacct
    15241 cgagcggatc gatggtgttg cctcgcgtct tggacatctt ctgccgctcg gcgtcgcgca
    15301 cgaggccgtg gatgtaaacc tgccggaacg gaatctcgcc cgtgcactcg atccccatca
    15361 tgatcatgcg ggccacccag aaaaacagga tgtcgaagcc ggtgatcagc agtgaagtgg
    15421 gatagtatgc cgccaggtcc tcggtctgct ccggccagcc cagtgtgctg aacggccaaa
    15481 ggccggaact gaaccaggtg tccaacacgt cgggatcctg gtagatgtcg tccgatgcgc
    15541 agtgcgcgca cttctccggc gtatccgcgg agaccgtgat cctgccgcac gtacggcaat
    15601 gccaggccgg cacgcgatgc ccccaccaaa gctggcgcga gatgcaccag tcgcggatgt
    15661 tgtacatcca ttcgaagtag gtcttgctcc aattctccgg tatgaactgg atgcgtccct
    15721 cttcgacggc ccgaatggcg ggctccgcca gcggtttcat cgtcacgaac cattgcgttg
    15781 agatcagcgg ctccaccgcc gttttgcaac gctggcactt tccgacggca acggtatacg
    15841 gctcgacttt gtcgagcagc ccgagcttat cgagatcttc gacaacccgc ttgcgcgcct
    15901 ccatgcgatc caggccggcg tacggacccg catcggctgt cattcgggcc tgctcgtcga
    15961 tgactttgat cttcggaagg tcgtgccgtc tgccggcttc aaaatcgttc ggatcgtggg
    16021 ccggtgttac ttttacgacc cccgtgccaa attcgggatc cgccagcgag tccaaaatga
    16081 tcgggatctc ccgatccatc agcggcaact gaacggtttt gccgtgcagg tcggtgtacc
    16141 gcccgtcctt ggggttgatg gcaacggcgg tatctcccag catcgtttcc ggccgtgtgg
    16201 tggcaaccac caggtagcgg tccgtgccct tgaccggata acggatgtgc cagagattgc
    16261 cggcggtctc ttcgtggttc acctcgagat cggaaagcgc ggtatggcag cggggacacc
    16321 agttcaccat gtactcgccg cgataaatca ggcccttttc gaatagccgg acaaagacca
    16381 ggcgcacggc tcgcgagagc ccctcatcga gggtgaagcg ctcacggctc caatcgcagc
    16441 tcgcgccaag ctggatcatc tgctgccgga tcgtgccgcc gtactcctgc ttccactccc
    16501 agactcgttt ctcgaacttc tcacgcccga gatcgtgacg ggtgagcccc tcttcggcca
    16561 ggcggcgctc cacaaccatt tgagtcgcga taccagcgtg gtccgttccc ggcagccaga
    16621 gtgtgttgta gccgagcata cggcgccagc gcaccgtcat atcgatctcc gaatgctcca
    16681 gcatgtggcc cgtatgaagt gaaccggtca cattgggagg cgggatgacg agagagaata
    16741 cccggccctg cgctttcggg gaagcgtgaa aaatgccgct ttccttccac cactgcgccc
    16801 accgcggctc aaaacgctgc ggctcataca ctttttcaat ttgcatcggg aattttcata
    16861 ataacatgca gccttttagc agcccctcgc gcgggagtgc tacaatgcgc gtctcaggag
    16921 gggctcatgc gtttgcattt cgttctctgt ctcattgggt tggccacagc tacagtcgcc
    16981 acgccgcagc aaaagaaaat cgtcgctgtg ggcttgtcgc ctgctgccat tgaggaactg
    17041 aatacggtca cgcccggcgt ccgggtagtg agagtggaat cggaccgtct tcccggcgaa
    17101 gtcgcggacg cggatgcgct ctttggcact gtcaatccgc gcctcattca ggcgggcaaa
    17161 aaactcaaat gggtgcagac gtacagcgcc ggcgtcgagt cctatatttc accagagttg
    17221 cagaacagcg gtattgtcct gacgaacgcc aagatcattc aggggccgga gatcgccgac
    17281 cacgccatgg cgctgctgct ggccctaacc cgggatctct acctgacgat tccgaaaagg
    17341 acctcccagg agtggccccg cggggaatac cgcccggtgg aactcaacgg caagacggcg
    17401 gttgtcatcg gagtcggcgg catcggcatg caaatcgcac agcgcgccag cgcattcggc
    17461 atggaggtga ttggcgtaga tccgaaaggc atctcctata cgaccttcct caaagaagta
    17521 gtccctccgg acaggttgga cacagtgctt ccgaaagccg atgtcgtctt cgtcagcgct
    17581 ccgttgaccg caaaaagcga aggcatgatg ggctcgaaac aatttgagct gatgaagaag
    17641 ggatcgtact tcatcgcggt atcgcgcggc aggctgtatg attcggcggc gctcgtcaaa
    17701 gctttggaca gccggcagtt ggccggagca gggctcgacg tcacgaaccc ggaaccgcta
    17761 cccaaaggcc acccgctctg gaatttcgaa aatgtgctga ttacaccgca catcgccgga
    17821 caatccgacc ggatcgggga gcgccggatg gagctgctca aggagaacat ccgccgcttc
    17881 gctaaaggcg aaacgctgat caacatcgtc gataaggcgg aggggtacta atccgaagtg
    17941 gcgtgtcgat ttcggagtac tccgggtctc acaactatcg aggctccagt cactattctc
    18001 gagaagctaa gctggagtgt ctgtcaacgc catggaagaa cttcggaaac acgttgaata
    18061 ccggctcacg cgccagggcc ggttcctgat cgctgaactt ctgcgccccc ataacgtact
    18121 gagtacatcc gttcggaacg gggggcagca gacttcgctc cgttacctcg ttaatcatca
    18181 gagttgcgag gcttcagacc accgggagcg gcagcggttg atttccgaaa tgggccagga
    18241 acaatatcac aatgttgctt gcacggaaat cggacttgac ccccaatacg tcgccatgat
    18301 gggcacggcg gcgaacatga actacgcaag tatcgtggat atggcggatg ataaccttcg
    18361 tgtcacggct gttgtaacag ccggcgttca agggaatgcc gcgtgtgcag gcgatcccgc
    18421 cggatggagg gagacggacg aaggttggca aaagatcgcg gcggcgggga ccatcaacac
    18481 gatgcttttc gtcaatcatc cggtcaccga aggagcactc gcccgagccg ctgtgacaat
    18541 gacggaagcc aagtccgcgg cactgcaacg gctggcaatt cgaagccttt attcgccgga
    18601 taccgcaacc ggaacaggca ctgaccagta ttgtgttgcg gcaacaatcg gtggcgcaaa
    18661 gccgcttacc tccacaagtc cgcacgtcat tctgggggag ttgatcggaa gagcggtccg
    18721 tgatgcgacg ctcgaagctc tgcgctggca gaacggactc gaatccagct acacacgcgg
    18781 tatctttcac gcacttggac aatacggtct gaacgagtca agctttttcg aggatattgc
    18841 cagccggctg acggatcggg aaatggagct tctccgtcgc aacaataaag ccgtattcta
    18901 cgagccaatg gtaggcgcct ctgcctatgc ggtcgcgtcg gtgctggacc gggtgcgtta
    18961 ccagacgctg ccgcccagtg cggctcgcga ggtaattcgg cagcaggctg cgactcttgc
    19021 gtcgaatctg gccgcgaaac cgcaagcctg ggcggagttc tggtcctgct tgcgcgaggc
    19081 cgacccagcg aaaccgcgag agctgattct cgatgcgatc gccgccggct ggtcttcaaa
    19141 gtggacctag cggcccttca cccgaatcca caggtcctgg ccggtgcggt tctgctcgac
    19201 cttgcgatcg gcgatccggt ttatcgggcg cacccggtcc ggctgatggg agcaacgctc
    19261 acatggcttg agaatcggct ccgagcggca cgcctggacg gttatggcgg gggcatcgcg
    19321 ctgttccttc ttctcgcagt gctttgggcc ggcggtatag cagccggttt gacagccttg
    19381 gcgcagtgga gtttctggct ctgccttgcg gtgcacttgt tcctggtcta ctcgctgctt
    19441 gcgttgcatg atctattgcg gcatgcctgg gcagttgagc gagcagctcg acagcacgat
    19501 cttgccgcga cacgggtagc agtctctcgt ctggtgggcc gcgacacgag ccgcatggac
    19561 ttcgccgcgt gccgcagagc cgcaatcgaa agcctcagcg agaacctggc cgatggcttc
    19621 gtcagcccct tgttctggta tgcaatcggt ggaattccag gtcttgttgt gttcaagatt
    19681 gtgagcacga tggattctat ggtcggatac aaaaccgaac gctatctgcg attcggctgg
    19741 tgcggcgccc ggcttgacga tctgatgaac ctgttgccgg ccagaatgac atggcttttg
    19801 attagtgtgg tcgcaagctt ctttcctcga tgctcggcac tcaaagcact acgaatcggc
    19861 tggcggtatc actcggtttt acccggtccg aactccggat ggagtgaggc ggccatcgca
    19921 ggagccctcc agcttcggtt gatcggaccg atatgggcgc agggcctgct ggtgacgaaa
    19981 gtttgggtgg gcgatccggc ggacccccca gcaggtgcgc gatctgatgt tccacgcgcc
    20041 gccgtcttca cggcgctgac aggattgctt gccgcggcgg cggcactcgc ttttattgct
    20101 gcgtatcctc gttgactcct gtaagcgact cgtgctagcg ttgagattcc ttaggtgccc
    20161 aaaacgggcc taacagggaa gtccggtgca aaaccggcgc ggtcccgcca ctgtaagtga
    20221 ggaatggccg tttgcaatat gccactcggt tcgaacaacc ggggaaggtg aaacggtctg
    20281 gcagctaacg acgctgcccc ctccgagcca ggagacctgc ctggggatta tgacggctga
    20341 ctttctgcgg agaacggaaa gaatgcgatt gatctccatc ctaattcgca ccccgattca
    20401 aaggctcttc gctccatcgt gggaagtctg tcgccaatca aaggaggaca gacctcatga
    20461 gcattgctac caaacgcggt gatggcggcc agaccagtct cgccggggga attcgcgtct
    20521 ctaaatcgtg cctacgcgtt gaagcgtacg gaacgattga tgagttgaac tcggtacttg
    20581 gctttgcccg ttcgatctgc gatgacgcag accttcggga acggaccagg ctcatccaac
    20641 gcgaattatt tcaagtcggc tccggtctcg ctaccccgtc cgtgagccgt aaaccgcaga
    20701 ttccggtccc tgctgaaatc gtcgattccc tcactgctca ggtccacgag atcgaggcga
    20761 cggaaggact actttcggat tggtcactcc ctggcgagca caccgccgct gcagcgtacg
    20821 atgttgcccg cacggtttgc cggcgcgccg agcgcaatgt agtgcggttg atggaagctg
    20881 gtgagacgtt cgacccgaat gccctcgcct acctcaatcg cttgtccgac ctgctctggc
    20941 ttttcggccg gaagatcgag cttgccgcag gagtgagcgg cgggctgcgc gccatgaacg
    21001 gaaaagaagg gccccgctgg tcccgggcct ggtaggggtc ccccgctgcc ggaagccgtt
    21061 tagcgatcaa ctgtcatgat tcggattcat cgattctcac acgccctgcc tctagccgtc
    21121 ctggttttgg ctatcggcat cattgccgtc gccgtggagc gcttcgcctc ttccctgggc
    21181 ggactgcgaa tcacgcgcga aggcgatggg tacggtagac ccgagatccg aaccgagtct
    21241 ttgaagtatc cgagagcggc tgtggatagc gaccttttcc gcgttcgaat cgcgaaacca
    21301 gcgcgccgga ttgtgtcgca atactggtcg atcgacgaat atctctattc ggtagtaccg
    21361 ccggagcgag tcgtcgcggt cagccaaagc gcctatgagc gccgcatcag caacgtgtat
    21421 ccgttcgcgg agcgctttcg tccggtgatt gcaaccgatc cggaaaaggt tcttcgccag
    21481 accccggacc tgattctcgt ttccaacagc gcacgtctcg atttctgcga tctcgtccgc
    21541 accaccggca taccgatata tcgcgctttc gcgatgttca cgacgctcga acaagtcgcg
    21601 gagacaattc gcctgactgg ttatctcacc ggggaagaca cagcggccgc taaggaagta
    21661 gatcgtttct ggtccgggat acggcgtgcc caggcgcgta agcctgccaa tgctccgcgc
    21721 cctcgaattt tagggatggg cggacggtct agctatggtg accagacatt gttccacgac
    21781 atcgttaaaa cgctcggggg gatcaacgta ggcgcggaag gtggattgcg cggctacaac
    21841 agtgtcaaca ccgagcagat tgtccaatgg aatccgcagt ggatcatcgc cggcgccgat
    21901 cgcggcaaga ccaaagaagt gctggaccgg atgttgcaag accctggaat cgcagtcacg
    21961 caagctgcga gagacggaca catcgtagtg ctcgaaaaca atgtgttcct gccggcgtcc
    22021 cccttcacta cgttgctcgt gaatgcgatt gctgaggcga tttatggata aacggcgcgt
    22081 tcggctcgag ctcttctatt tggtcctgct gcccgtattg gcgctatccg gcgtcatcgc
    22141 cattggtata ggaagcacgg aagtgtcctg gcggacgatc cttggagtcg ccgctcagaa
    22201 actgctgccc gggggttggg tcgataccag cggattgacc caggcagata ctgtggtcgt
    22261 ttggcttatc agagtgccga gagtgttggt cgcggccttt gtcggggccg ggctctcagc
    22321 cggcggcgca gtcatgcagg ggctattccg gaacccgctt gcggaatccg gcctggtggg
    22381 tgccggttcg ggcgctgcgc ttggttcggt gatcgctttc atcgccgggt ggacggtaag
    22441 gtccattgtc tcgttgccag tggcagcgat gatcggtgcg ctgatggccc tcgttgtggt
    22501 gtatgccatg gcgactcgcg gtggcgttac gcctgtcgcg accttgcttc ttgccggcgt
    22561 cgcggtgact tcccttctca cggccgtgtg gagtctgctg ctgtccatca acatcgtaaa
    22621 ctggcaaatc gcacaagaag ttcttttttg gattatgggc ggcttggacg cacgctcgtg
    22681 gacgcacgta tggctgtgcg caccgtttgt gactctcggc attgtcgccg caattgtcca
    22741 ggcacgcgaa ctcgacctga tgcagcaggg agaggaaacg gcggcggcat tcggcgtgaa
    22801 tgtcgagcac tccaagcgaa tgttgattct caccgcttct gtgctcacgg gggcgtgcgt
    22861 gggggtcgcc ggcatggttg cttttgtcgg cctggtggtt ccgcatgcgg tacggctatt
    22921 gatcgggccg tcgcatcgcc cgctgcttcc ggcctgttca atcgccggag cggcgtttct
    22981 catcctgtgt gacctggcgg cgcggaccat tcatccgcca acggaaatac gtcttggcgt
    23041 gatcacttcg ttagttggcg gcccgatttt tattgcgctg ttgatgcgcc gctaccgcga
    23101 ggttggactt gcatgagcgt gctcgacgct acttcaatct cggtcagccg cggcggccgt
    23161 cagattctca gcgacgtgac catcagcctg tcgtccggcg agttcctcgc gattatcggc
    23221 ccgaacggaa gcggcaagag ttcgctgctc cgcgtgcttg ctggaatctg gccggcggaa
    23281 tcgggatccg tgtctctcga cggaacaccc atcggcgata tgtcgcgtcg cgatatcgca
    23341 cggcaagtgg cttttgtgcc gcaggataca cgagtcgact ttgctttcgc ggttgaggag
    23401 atcgtagcga tgggacggca tccacatcgc ggccggttca ccagtgaacg ggaaacggac
    23461 cgccgcgcga tcaatctcgc gctcgaacgt tgcgacgtca gttcgctgcg ccaccgcctt
    23521 gtcaacacac tctccggagg cgaacgtcag cgtgttctga ttgcgcgcag ccttgctgtc
    23581 gagcccgcgt tcctcctgct cgatgaaccc accgcaaatc tggacattca acatgccctc
    23641 gaaattcttg acctttgccg gtctctcgcg gatggcggac aggccatcgc attcgcaagc
    23701 cacgatatca acgcggtgtc ccgatacgcc tcgagtgtgg cgctgatgga atccggccgc
    23761 attgtggctg cgggcacacg gaaccaggtc ctgactcccc gggccgttcg ggatgtattt
    23821 ggtgtcgcga tcgaagtcct ggccgcctcc aacggacaat cactctacct gtttcatcga
    23881 aggaacaacc agatatgaca cgattcctac tcttgcttgc atgcttctcc tcttccctga
    23941 ttgcgcagga aggaaccatc ctgcgcgggc gtgttgccga ccttcaaggc gcaacgattc
    24001 ccgatgccag ggttcgcctc tatcgccagg acacgggtgt ggccctccag actcgaagta
    24061 a
//
LOCUS       DASTMK010000176        10659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_360903, whole genome shotgun sequence.
ACCESSION   DASTMK010000176 DASTMK010000000
VERSION     DASTMK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360903"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..805)
                     /locus_tag="VFQ79_07580"
     CDS             complement(17..805)
                     /locus_tag="VFQ79_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139553.1"
                     /translation="MRVLAAVLLSIVAGAPLARSEDLTFDQRVEIVRGLTAEYATAKI
                     VLPRSKKTLVYKSTGEYDKQQWADVTKEFGPAARVGDLVQVSKVSIENDRIVLQINGG
                     FKGGRKWYERIEVGMGTRTTPISQGQSNAPGGTELAVSFPKRTPPLEAAAIKKMLAPI
                     LDFEKRSATELYVENLPAPIQAAIKEKRAAEGMDRDQVVLAMGKPRHKVRETLDGEDF
                     EDWIYGQPPGKITFVTFQGSKVVRVKDEYAGLGGTVVPPLEPQR"
     gene            complement(802..1659)
                     /locus_tag="VFQ79_07585"
     CDS             complement(802..1659)
                     /locus_tag="VFQ79_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU0139554.1"
                     /translation="MGATERKRIVGLALRVAVFTFLALVGMVVFVTILFNIGGYLVGA
                     TLGTFASAAVANAIALRIWEQGRLSHIGLAWTPGSGRNIGLGLAGGVAAALLVVGGPL
                     LTGAAELKPLAENPFNFGSLVFVSVVLLFGAAGEELLFRGYGFQVLVAVLGPYATVLP
                     VAVLFGLAHANNQNATALGLFNTILWGVIFGYAFLRSGDLWLPIGLHFGWNWTLPLFG
                     TNLSGFTMGITGCETQWNAGELWSGGNYGPEGGLLTTAVLIPLFVFLYKAPIRPQTSL
                     LLQSFEEPS"
     gene            complement(1663..2673)
                     /locus_tag="VFQ79_07590"
     CDS             complement(1663..2673)
                     /locus_tag="VFQ79_07590"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139555.1"
                     /translation="MKITSKSPCRVDMAGGTLDIWPLYLYHTGAVTLNFAVDRYTSCS
                     IQTRRDSQIVLKSQDQESEESFGSLAELQSAKKYKLPLLACLVRFFQPRTGIDITTNS
                     EVPAGGGISGSSSLIISTSSALNRLTGSGYNIEKIREISQNVEAQIVRVPTGCQDYYP
                     AMYGGVSAIELGPAGIRRIPLSLDLDDLNERVVLAYTGEPRNSGINNWEVMKAYIDGD
                     RAVHRNFDKIAAIANSMRQALAQSDWKEVARLLREEWSHRKKNAPGITTPLIDRLVEA
                     TRRAGAVGAKVCGAGGGGCVFFLVERGAKQRVTRIIEQEGGEILPVKVAPRGVQVRTV
                     AE"
     gene            2704..3255
                     /locus_tag="VFQ79_07595"
     CDS             2704..3255
                     /locus_tag="VFQ79_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0139556.1"
                     /translation="MNYYWSMPVRRILQLGDTSLRIVSAPLESAGEAIPLLSDLRDTL
                     HDFQRVRGFGRGISAVQIGDARRVIYLEFEGKPYSLINPELVWHSTATFRLWDDCFSF
                     PELLVYLERWYSVRVRYLDETAKQRELEAEGSLSELLQHEIDHLDGILAVDRAIDRNS
                     LCTRDEYERRKAIPCGTGTGTPQ"
     gene            3337..3708
                     /locus_tag="VFQ79_07600"
     CDS             3337..3708
                     /locus_tag="VFQ79_07600"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0139557.1"
                     /translation="MNERRIQPRLLCADLVDVEWKDGMGRRHSAIANLEDISLSGACL
                     QMEEAIPLHAKVSINYPKGELQGACRYCVYREIGYFIGVQFEPGVKWSRKNFKPQHLL
                     DPRRLLMRENSRTADVAGSRS"
     gene            3960..4322
                     /locus_tag="VFQ79_07605"
     CDS             3960..4322
                     /locus_tag="VFQ79_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139558.1"
                     /translation="MVLASAMLALVMYQWWRRTAAYPARRSDPDVDLPGNAEEIVEVP
                     PGESSNGTGYPAIRHELALAADHVHSIRVLLEVSRSHDQPIPLSVLRNLDLVGKHLRE
                     LERRSSDAAAESACGLLR"
     gene            4627..5433
                     /locus_tag="VFQ79_07610"
     CDS             4627..5433
                     /locus_tag="VFQ79_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0139559.1"
                     /translation="MAAFDKILVPVDLSHRSIGAARYACSLAEDFGSELVFLHVIRSG
                     WPLEAPAKEIRDQILGLMKLTSARFLVREGLPVATIIDTAAAEKADLILMPTRGVNTM
                     SRLLGRSITTQVLRAAPCTVWSGVDDLSIFSRRPIRNVLCGLSLGPRTSKVLGCAAGL
                     AAKFHATLSLVHATKALRGAPGYPCDDWRFQIRKLAKDGIQAAQEEVGTNAEVWVESG
                     TPVRAVPALAEILRSDLLVIGKSPDLRLLPDLRSVSYDIVNRAPCPVASV"
     gene            complement(5437..6300)
                     /locus_tag="VFQ79_07615"
     CDS             complement(5437..6300)
                     /locus_tag="VFQ79_07615"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0139560.1"
                     /translation="MENVYSDYVDAIFAVQKMGERLPLGGFDPLMAPPAISSDKKVMG
                     FAPHPDDMEIGGVLPVRLRRLGMPVLSVAVTLGSNKARRPGRLAELKGASKFLGYELV
                     EVQEGGFERINPAGRSGDPENWKRSVQRIAALISEHRPAVIVVPHSEDFNSTHIGTNL
                     LVMEALASIGPSFTCQVALTEFWFPIRNPNVMLEVEAPLVADLVGAVSYHVGEVERNP
                     YHLTLVPYMIDNVRRAELVLGIGAAAPPWKMAILMRVEHWNGTQLKPSFEGGRLIAAG
                     DTKGLNEVLSL"
     gene            complement(6320..7693)
                     /locus_tag="VFQ79_07620"
     CDS             complement(6320..7693)
                     /locus_tag="VFQ79_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139561.1"
                     /translation="MDLPKLVPVFDPDFRPAVLARRRYEKDAAGGRPIRIAIEQDENT
                     YVFNRTILNDDSSQGLSRNLRFLERDLKFLLWAVGGWRVHIDAPELLVGLLRDYHYRY
                     SWTQLFDAEVMGPTVYGRPFQIEHAEGNAFPEAAHQAISLGRHRGGCRLAIDLGASDI
                     KAYAEQDGKAAFAGEFPWDPKPQCDPAYHYQHVSNALRTAAATLPRVDCIGVSSAGVY
                     VSNEPRIASLFRGVPKDRFQAEIVPLFHRIQSDFGGVPLVVANDGDVAALAGSMSLGT
                     NRVLGLAFGSSLAVGYVDGNGNITSWLNELAFVPIDYNPRAPADEWSLDRGCGVQYLS
                     QQAVDRLLKPAGIEVPAGMPVPVRLEQVQALMNQGDPRAEKIYRTIGVYLGYALAHYA
                     SYYDIGTALIMGRVTTGPGGEMILDIATEVLAREFPEISIDLRLPDEASKRVGQAVAA
                     ASLPQIV"
     gene            7830..9875
                     /locus_tag="VFQ79_07625"
     CDS             7830..9875
                     /locus_tag="VFQ79_07625"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139562.1"
                     /translation="MTRILTFILLLAGAAPAAELFRDDFSGFPPGWLTYPVGQLNGAI
                     QEYHYLPYRGLPLGPWANTIVHLDAWVVGDEDGENYLEQHLMGSMASQFTNPLFITGD
                     PEWSDYTVSAKVKPLSFADFAGIVFRYHTNRHYYLFALTGGNKARLTVRLPLEKSFRV
                     WEWRDLAVKDFPYEAKRYYELRVENNGPVIRAFIDGKPVAEVNDSEILKGKVGVTASV
                     PARFKDFAVSASDATVQQITARISAREQELATLRAGNPQPRLWKRFTTPGFGAGRNVR
                     FGDLDGDGVIDMLIAQNIPRVRGDAFDHISCLTAVNLDGKILWQSGRPDPRNGLLTND
                     TPFQIHDLDGDGRNEVVLVRDFKLQVLEGRTGKPLKSEWMPTAPPENRERPYEMETGD
                     SIAFANVSGNKTARDIILKDRYRNFWLVDSELKPLWRGQGQTGHYPYPVDVDGDGRDE
                     ILIGYALWSHDGRLLWSRDKELRDHSDAIAMGNLSPDPKAPLRAYSSGSDEGFLLFDH
                     EGNILKHVRIGHAQSPSIAKYRMDVPGLQYMTVNFWKNPGIVTLFDWDGNMLAQEEPI
                     HSGSVLLPVNWRGDGQEFALLSGNVREGGMIDGQLRRVVMFPDDGHPDLTANVLNVTG
                     DARDEIILWDQERVWIYTQDRPFSGKKIYAPRRNPDYNESNYRLNVSFPEWQPVSGK"
     gene            9851..>10659
                     /locus_tag="VFQ79_07630"
     CDS             9851..>10659
                     /locus_tag="VFQ79_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0139563.1"
                     /translation="MAASERKMILALALLLVSASDALDELEQRARLSLESIRHADTLA
                     DAAAQRPALRARLRGSLGLDLLPPAADLKARVVGTLSRGGCRIEKIVYETLPGVLVPA
                     HLYLPERVDSRAPALLFYPGHWWADSKMRPDFQAFCLTMARLGFVVLSWDPFGQGERG
                     VSSRDHRRIEMLLAGVSQQGLAEYETECALRYLLSRREVDPKRIGITGASGGGYNTWI
                     TSALDDRIAAAVPVVGTSEFYEQIHVTRPLDWYRAAEHCHFVPGLIRYANN"
BASE COUNT         2104 a         3223 c         3136 g         2196 t
ORIGIN      
        1 ggatgtttat ccgaaactac cgctgtggtt cgaggggggg caccacggtg ccgccgagtc
       61 cggcgtattc gtccttgacg cgtacgacct tgcttccctg aaatgttaca aaggtaatct
      121 ttccgggagg ctgcccatag atccagtctt cgaaatcttc gccatcgagt gtttcacgaa
      181 ctttgtggcg tggtttaccc atggcgagga ccacctggtc gcgatccatt ccctccgcgg
      241 cgcgtttttc tttaatggcg gcctggatgg gggcgggcag gttctcgacg tatagttcgg
      301 tcgcgctgcg cttctcgaaa tcgaggatcg gcgccagcat cttcttgatc gcggcagcct
      361 cgagcggggg agtccgcttc ggaaaagaaa cggcgagttc cgtaccgccc ggagcattac
      421 tttgtccttg cgagatcgga gtggtgcgcg ttcccatgcc cacctcgatc cgttcgtacc
      481 acttgcgccc gcccttgaat ccgccgttga tctggagcac gatccggtcg ttttcgatgg
      541 acactttgga aacttgcaca aggtcgccaa cgcgtgcagc cggcccgaat tcctttgtga
      601 catcggccca ttgctgcttg tcgtattcgc cggtactctt ataaacaaga gtcttcttcg
      661 accttggcag cacgatcttg gccgtagcat actcggccgt aagcccccgt acgatctcca
      721 cacgctgatc gaaggtgaga tcttctgaac gggcgagcgg agcaccggcg acgatcgaaa
      781 gtagaaccgc ggccaggacc ctcatgatgg ctcctcaaaa gattgcagca gcaaagaggt
      841 ctgcgggcga atcggcgcct tgtaaaggaa aacgaacaac ggaatcagca ccgcggttgt
      901 aagcaggccc ccctcgggac cgtaatttcc gccgctccat aattcgccgg cgttccattg
      961 cgtctcgcaa cccgttatcc ccattgtaaa cccgcttaga ttggttccga atagcgggag
     1021 tgtccaattc cagccaaagt gcagccctat cggcagccag aggtcgccgc tccgcaagaa
     1081 tgcatatcca aaaatcacac cccaaagaat cgtgttgaat agacccagcg ctgttgcatt
     1141 ctggttgttc gcatgagcca gtccaaacag gacggcgacg gggagtacgg tggcgtacgg
     1201 ccccaatacg gcgacgagca cctggaagcc atatccgcgg aacagcagtt cctcgcccgc
     1261 ggctccgaac aagagcacga cggaaacgaa gaccaggctt ccgaaattga acggattctc
     1321 cgccagcggt ttcaactcag ccgcgccggt cagtaggggg ccgccgacta caagcagcgc
     1381 ggccgcaacg ccgccggcca gccccagccc gatgtttcta ccggagcccg gagtccaggc
     1441 gaggccgata tgcgaaagcc gtccctgctc ccagattcgc agagctatcg cattggcgac
     1501 ggcggcggaa gcaaacgttc ccagtgtggc acctacgaga tacccgccga tgttgaagag
     1561 gatggtgacg aacaccacca tcccgaccag ggcgagaaac gtgaaaacag cgacgcggag
     1621 cgcgagacca acaatacgtt tccgctcagt cgcgcccacg cgctactccg ccaccgttct
     1681 tacctgaacg ccgcgcggcg caaccttcac cggcaaaatt tccccgccct cctgttcgat
     1741 aatgcgggtg acccgttgtt ttgccccgcg ttcgaccaga aagaagacgc accctccgcc
     1801 gcccgcgcca cagacctttg cgcccaccgc gccggcacgc cgcgtagcct ctaccaacct
     1861 gtcgatcagc ggtgtggtga tgcccggtgc gtttttcttc cggtggctcc attcttcccg
     1921 cagcagcctg gctacttcct tccagtccga ttgcgccagc gcttgccgca tggaattcgc
     1981 gatggccgcg atcttgtcga aattccggtg tacggcccgg tccccatcga tgtacgcttt
     2041 catcacttcc cagttgttga tgccggagtt ccggggctcg cctgtatagg ccagcaccac
     2101 gcgctcgttc agatcgtcga gatccagaga tagcggtatg cggcgaattc ccgcgggccc
     2161 caactcgatg gcgctgacac cgccgtacat cgcgggataa taatcctggc agccggtggg
     2221 aacgcgtacg atctgcgcct cgacgttctg cgaaatttcc ctgatctttt cgatgttgta
     2281 cccgcttccc gtcagccggt tcagggcact cgaggtggaa atgatgagcg acgacgagcc
     2341 ggagattcct cccccggcgg gcacctcgga attcgtagtg atatcgattc cggtcctggg
     2401 ctgaaaaaac cgcaccaggc aggccagcag gggcagctta tactttttcg ccgactggag
     2461 ttcggcgagg gagccaaacg attcttcgct ctcctgatcc tgcgatttca gcacgatttg
     2521 tgagtcccgc cgcgtttgga tggagcagct cgtataccgg tccaccgcga aattcaatgt
     2581 cacagcgccc gtgtgataga gatacagcgg ccagatgtcg agcgtcccgc cggccatgtc
     2641 tacccggcac ggcgacttgg aggtgatttt catagctaag ccgtaatctt aacggattca
     2701 acgatgaact actattggag catgcccgtg cgccgcattc tccagttggg agacacttcg
     2761 ctgcgcattg tttccgctcc ccttgaatcc gccggcgaag caatcccgct cctgtccgat
     2821 ctgcgcgata cgctgcacga tttccagcgg gttcgcgggt tcggccgagg catcagcgcg
     2881 gtgcagatcg gcgatgcccg aagggtgatc tacctggagt ttgaaggcaa accttacagc
     2941 ctgattaatc ccgagttggt gtggcatagt accgcgacgt tcaggctatg ggacgactgt
     3001 ttttcgtttc cggagctgct ggtgtatttg gaacgctggt actccgttcg tgtccggtac
     3061 ctggatgaaa ccgcgaaaca gcgggagttg gaggcggaag gttccttgtc ggaactgttg
     3121 cagcatgaga tcgatcacct ggatggaatc ctcgccgtcg accgcgctat cgaccggaat
     3181 agcctctgca cccgggacga atatgaacgt cgtaaggcga ttccctgcgg aacaggcacc
     3241 ggaactcccc aataagcacc ccgtatgccg tatagggcaa aatgttaggc cccgttcgct
     3301 aaacattcga attttgtgga ccgataaaac agttgtatga acgaaagacg tatccaaccg
     3361 cggttgcttt gcgcagatct cgtcgatgtg gaatggaaag acggtatggg tcgaaggcac
     3421 tcggcgatcg cgaatctgga ggacatttcg ctttcgggag cctgccttca gatggaggaa
     3481 gcgattccac ttcacgccaa agtatccatc aattatccga aaggggagtt gcagggcgcc
     3541 tgccgctatt gcgtctatcg tgagatcggc tacttcatcg gcgttcagtt cgaacccggc
     3601 gtgaaatggt cgcgcaagaa cttcaaaccg cagcatctgc tggatccgcg ccggcttctc
     3661 atgagagaga attcccggac cgcggatgtc gccggctctc gtagctgaca cgggcgattg
     3721 ttcctcccac gtgcctgaaa gtaggcaccg gtgtctgttt tcggacaccg gaattacctt
     3781 gctacagtgt caaacaagcg attactgctc tggcaggcta tgtgctcttt gcgcgttgcg
     3841 atatgtcgga aatcgcgaaa ctttggctcg cgaccacaac aattcttgtg ctattcggca
     3901 tgggactgga agggcttagg gtagttcgat ggacacatcc tcttaactgg gtccttttga
     3961 tggttctggc ttcggcaatg ctggcgcttg tgatgtacca atggtggcgt cgaacggccg
     4021 cctatcccgc gcggcgctcc gatcccgatg tggatttgcc gggaaacgcg gaggagatcg
     4081 tcgaagtccc acctggggag agttccaacg gcaccggata cccggcaatc cggcacgaat
     4141 tggcactggc ggcagatcat gtgcatagca tccgggtcct tttggaggta agccgctctc
     4201 acgatcagcc tattccactc tccgtgcttc gcaatctgga cctggtgggc aaacaccttc
     4261 gcgaactgga gagacgctcg tcggatgccg cggcggaatc cgcatgtgga ttgctgcgtt
     4321 aagagagtga agtgagaaac tccacgcaat accggttgcg gcggtgcatt gcgcatctgc
     4381 cagcggggta tcgcgaagta tggcatctcc atcaaataga gcaaagggac tccaggtgga
     4441 tcgcctgccg gttgcggcta agctcctacg aggtgcaatt acggctctgg cgggccaaac
     4501 gaatgttgcg tgtaattctg cgtcgggagt ttggaagccg cttgcgcttg aggccgcgtc
     4561 tgctgcatgg accgcaatga ggcgcttctc cacagaggtg ggcatgtaat tgcaatgtaa
     4621 tggtagatgg cggcattcga caagatactc gttcccgtgg atctgtccca ccgatccatt
     4681 ggtgctgccc gctatgcgtg cagcctcgcg gaggacttcg gttccgaact cgtgtttctg
     4741 cacgtaatcc ggtccggctg gcccctggaa gctccggcaa aggagattcg cgatcagatt
     4801 ctcgggctca tgaaattgac gtccgccagg tttctggttc gtgagggttt gcctgtcgcc
     4861 acaatcatcg acacggcggc cgccgaaaag gcggacctca ttctcatgcc gacacgtggc
     4921 gtcaacacga tgtcgagatt gctgggacgg tccatcacca ctcaggtgct tcgcgctgct
     4981 ccgtgtacgg tctggagcgg agttgacgac ctctctattt tttcgcgccg tcccatccgg
     5041 aacgtactct gcggcttgtc gctgggccca cggacatcga aagttctggg gtgtgctgcc
     5101 gggctggcag cgaaattcca cgcaaccctt tcgctggttc atgcgacgaa ggccctgcgg
     5161 ggagcgcccg gctacccatg tgacgactgg cgcttccaga ttcgcaagct ggccaaggac
     5221 gggatccagg cggcacaaga ggaagtgggt acgaatgccg aggtgtgggt ggaatccggc
     5281 acccccgtac gcgccgtgcc cgccctggcg gaaatcctgc gaagcgatct gctggttatt
     5341 ggtaagagcc cggacctccg gctgctcccg gacttacgtt ccgtttctta cgatattgtg
     5401 aaccgggcac cctgcccggt tgcaagcgtc tgatcgttac agcgaaagca cctcgttcaa
     5461 gccctttgtg tcaccggcgg cgatcaggcg tccaccttca aacgatggct tcagttgcgt
     5521 gccgttccag tgctcgacgc gcatcaggat cgccatcttc cacggtgggg ccgctgcgcc
     5581 gataccgagc accagttccg cccgccggac gttgtcgatc atatagggga ccagcgtcag
     5641 gtggtacggg ttgcgttcga cctcacccac gtgatacgaa acagcgccca ccagatcggc
     5701 aaccagcgga gcttccacct ccagcatgac gttcggattc cggatcggga accagaattc
     5761 ggtcaaagcc acctggcacg tgaaggacgg accaatactg gcgagcgctt ccatcacgag
     5821 caggttcgtt ccgatatgag tgctgttgaa gtcttccgag tggggaacga cgatcaccgc
     5881 gggccggtgc tcgctgatca gcgcagcgat ccgctgaacg ctgcgtttcc agttttcggg
     5941 gtcgccgctg cgcccggccg gattgatgcg ctcgaagccg ccttcctgca cttcgacgag
     6001 ttcgtagccc agaaacttgc tggcaccctt caactcggca aggcggcccg ggcgtcttgc
     6061 cttattcgat ccgagcgtca cggcaacgct cagaaccggc attccgaggc gccgcagccg
     6121 gaccggcaga acaccgccga tttccatgtc atcaggatgc ggagcgaagc ccatcacttt
     6181 cttatccgat gaaatcgccg gtggggccat caacggatcg aatccgccca gcggcaggcg
     6241 ttcgcccatc ttctgaacgg cgaatattgc gtctacgtag tctgaataaa cgttctccat
     6301 tgcgcttcct cgtacggggt taaacaattt gcggcagcga cgcggcggct acggcctgcc
     6361 ccacgcgctt gctcgcctca tccggcaggc gcagatcaat cgagatttcg ggaaactcgc
     6421 gggcgagcac ttccgtcgcg atatcgagga tcatctcgcc gccggggccg gtggtcacgc
     6481 ggcccatgat cagcgctgta ccgatatcgt agtagctggc atagtgtgcc agcgcatagc
     6541 cgagatatac tccgatggtg cggtagatct tctccgcgcg cgggtccccc tgattcatca
     6601 acgcctgcac ctgctcgagc cgtaccggca ccggcattcc ggccggcacc tcgatcccgg
     6661 cgggcttcag caggcgatcg accgcctgct gcgacagata ctgaaccccg catccgcggt
     6721 cgagcgacca ttcgtcggcc ggtgcgcgcg ggttgtaatc gatcggcacg aacgccagtt
     6781 cattcagcca ggaagtgatg tttccgttgc cgtcgacgta gcccacggcg aggctgctgc
     6841 cgaaagcgag ccccagtacg cggttcgtgc ccaaggacat cgagccggcc agcgcggcca
     6901 catctccatc gtttgccacc accagcggca ctccaccgaa atcactctga atgcggtgga
     6961 agagcggcac gatctccgcc tggaaccggt ccttcggcac accccggaac agcgaggcga
     7021 tgcggggctc attggaaacg taaactcccg ccgagctgac gccgatgcaa tcgacgcggg
     7081 gcagcgtggc cgcggcagta cgcagcgcgt tgctgacgtg ctggtaatga tatgccggat
     7141 cgcactgcgg tttggggtcc cacgggaatt cgcccgcaaa ggccgccttg ccgtcctgct
     7201 cggcatacgc tttgatgtcg ctcgctccca gatcgattgc caggcggcat ccgccgcggt
     7261 ggcgccccag cgagatcgcc tggtgggctg cctcgggaaa cgcgttgccc tccgcatgtt
     7321 cgatctggaa aggtcttccg taaaccgtcg gtcccatcac ttccgcatcg aacagttgcg
     7381 tccacgaata ccggtagtga tagtcgcgca ggaggccgac caggagttcc ggagcgtcga
     7441 tgtgcacgcg ccacccacca actgcccaaa gcaggaactt caggtcgcgc tcgagaaagc
     7501 ggagattgcg ggacaggccc tggctgctat cgtcgttcag gatggtccgg ttgaacacat
     7561 atgtgttctc atcctgctcg atcgcaatcc ggatggggcg tccgccggcg gcatctttct
     7621 catagcggcg gcgggccagt acggcgggtc tgaaatccgg atcgaacacc ggcaccagtt
     7681 tcggaagatc caatgcaaat ctccttaatt gtattgttat ccccagaata ccgggaacct
     7741 ggcagcaagt ctaacggccg ctgcggttat gctgataggg agagtttgga ccttacgcgc
     7801 cttgaatcag gtcctgcttc caatctgata tgacccgtat actgaccttc atcctactgt
     7861 tggccggcgc tgcaccggcg gcggaactat tccgcgatga tttttcgggc tttcctccgg
     7921 gctggttaac ttatccggtc ggacagctca acggcgccat acaggaatac cactatcttc
     7981 cttatcgcgg cttgccactt ggaccgtggg caaacacgat tgtccacctg gatgcctggg
     8041 tcgttggcga cgaggacggt gagaactacc tcgagcagca cctgatggga tcgatggcaa
     8101 gccagttcac gaatccctta ttcatcacgg gcgatcccga atggagcgac tacaccgttt
     8161 ccgccaaagt gaagccgctg tcttttgccg attttgcggg aatcgtattc cgctaccaca
     8221 ccaaccggca ctactatctg tttgctctta cgggaggcaa caaggcgcgc cttactgtgc
     8281 ggctgccact cgaaaaatcg ttccgtgtgt gggagtggcg cgatctcgcc gtcaaagatt
     8341 ttccctacga ggcgaagcgc tattacgagt tgcgagtcga gaacaacggc cccgtcatac
     8401 gcgctttcat cgacggcaaa ccggtggcgg aagtgaacga ctccgaaatc ctcaaaggta
     8461 aggtgggggt caccgcgagt gtaccggcgc ggttcaagga tttcgccgtc tcggcctccg
     8521 atgccaccgt tcagcaaatc accgcccgca tttcggcgcg cgaacaggaa ctcgcgacgc
     8581 tgcgcgccgg caatccgcag ccgagacttt ggaagcgatt caccactccc ggatttggag
     8641 ccggacgcaa tgtccgcttc ggcgatcttg acggcgatgg tgtcatcgat atgctgatcg
     8701 cacagaacat cccgcgtgtt cgcggcgatg ccttcgatca catcagttgt ctcaccgctg
     8761 ttaacttgga cggaaagata ctgtggcaat ccggccggcc cgatccccgc aacggcctgc
     8821 tgacgaacga tacgcctttc cagattcacg acctggatgg agacgggcgc aatgaggtgg
     8881 tgctggttcg cgatttcaag ctacaggtgc tcgaaggccg caccggcaag ccgctgaagt
     8941 ccgaatggat gccaacggcg ccgcccgaaa atcgtgagcg gccctacgag atggaaaccg
     9001 gagactccat cgcgttcgcc aacgtgagcg ggaacaagac cgctcgcgac atcatcctca
     9061 aggaccggta tcgcaatttc tggctggtcg actccgagtt gaaaccgcta tggagaggac
     9121 aggggcagac cgggcactat ccctacccgg tggatgtgga cggcgatggt cgtgatgaaa
     9181 ttctgatcgg gtacgcgtta tggagccacg acggccggct gctttggagc cgggacaagg
     9241 agttgcgcga ccactcggac gcgatcgcca tgggcaacct cagcccggac cccaaggcac
     9301 cattgcgcgc ctattcgagc ggcagcgacg aaggcttcct gctgtttgat cacgagggca
     9361 atatcctgaa gcatgtgcgc atcggccatg cacaaagccc cagcatcgcg aaataccgca
     9421 tggatgttcc cggcctgcaa tatatgacgg tgaacttctg gaaaaacccc ggaatcgtta
     9481 cgcttttcga ttgggacggc aatatgctcg cgcaggagga acccatccat agcggtagtg
     9541 ttctgcttcc cgtgaactgg cgcggcgacg ggcaggaatt cgcgctgtta tcgggcaatg
     9601 tccgcgaggg cggcatgatt gacggccaat tgaggcgtgt cgtgatgttc cccgatgacg
     9661 gccatccgga cctgacggca aacgtcctga acgtcacggg cgatgcgcgc gacgagatca
     9721 tcctgtggga ccaggaacgt gtgtggatct acacgcagga tcgtccgttc tccggcaaaa
     9781 agatctacgc tccccggcgc aaccccgatt acaacgaatc gaactaccgt ttgaacgtct
     9841 cctttccgga gtggcagcca gtgagcggaa aatgattctg gcgctcgcgc tgctgcttgt
     9901 ttccgcaagc gatgctctcg atgagctgga gcagcgagcc cggctttcgc tcgaaagcat
     9961 acgccacgcg gatacgctgg ccgatgcggc ggcgcagcgg ccggccttgc gtgcgcgcct
    10021 gcgcggctct ctgggcctgg acctattgcc gcccgcggcc gatctgaaag cgcgagtcgt
    10081 tggcacgctc tcgcgaggcg gctgccgcat cgagaagatc gtctatgaaa ccctgccggg
    10141 ggttctggtt ccggcccatc tttatctccc ggagagggtc gactcgcgcg ctccagccct
    10201 tctcttttat ccggggcact ggtgggccga cagtaaaatg cgtccggact ttcaggcgtt
    10261 ctgcctgacg atggcacggc ttgggttcgt cgtgctctcc tgggacccct ttgggcaagg
    10321 cgagcgcggt gtctcgtcgc gcgatcatcg ccgcatcgag atgctgctgg ccggcgtctc
    10381 gcaacaggga ctggccgaat acgaaaccga gtgcgcgtta cgttatctgc tttcgcggcg
    10441 tgaagtggac ccgaaacgaa tcggcatcac gggtgcgtcg ggcggcggct acaacacatg
    10501 gatcacttcc gctctcgacg accgcatcgc cgccgcagtt cccgtcgtcg gcacaagcga
    10561 attctacgaa cagattcacg tcacccgtcc tctcgactgg tatcgcgcgg ccgagcattg
    10621 ccactttgtt cccggcttga tccggtatgc gaataacca
//
LOCUS       DASTMK010000177         9753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_9849, whole genome shotgun sequence.
ACCESSION   DASTMK010000177 DASTMK010000000
VERSION     DASTMK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9849"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFQ79_07635"
     CDS             complement(<1..427)
                     /locus_tag="VFQ79_07635"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU0139564.1"
                     /translation="MRKIVGKTVTLAAAGLLFIMPVAFAQEETDQQRTPAPQERTLPQ
                     QERTAPQEETTVPQTQPQTAESERADQARTATAGDKLSRGERKFLSKAAQGGMAEVQL
                     GQLAQEKAASDEVKQIGEKLVEDHSQANDQVQQIATAKGI"
     gene            complement(746..3865)
                     /locus_tag="VFQ79_07640"
     CDS             complement(746..3865)
                     /locus_tag="VFQ79_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0139565.1"
                     /translation="MFLSDLSIKQPVFATMLAVSLVTLGIYSYRELSVDLFPDVEIPV
                     LTIQTLYPGASPETVEREVTKRIEEAVNTISGVRHISSTTTEGFSVIVVEFRLGTNIH
                     TAQQDTQAKIDGIRADFPLSMEQPVVQRIDFNAMPVVSIAVESPTMDIKALSSIAEKQ
                     IKRRLENVPGVGQVNLIGLARREIQILVDRNKLKALGLTYAEVSAALRRENLDVPAGK
                     LDQGQIEPLVRVSGKFQQVEEFRDLIVATRNGRPVFLPEVARIVDSIEERRSASLVDD
                     RQALALDIVKQSGANTVDVADGVEAAVAQMNAEFPSHIRLRTVVDRSIFIRESVEDVQ
                     LTLIIGAALTVLIVFLFLNSWRSTVITGLALPVSVISTFIIMAALGFTLNILTLMGLS
                     LSIGLLIDDAIVVRENIVRHMHAGADHFTAAREATAEIGLAVMATTFTIVAVFVPVAF
                     MGGIIGKFFYEFGITVGFAVLVSLYVSFTLDPMLSSRWYDPAADPSIPRSVVGRALDK
                     LNAQIEKLRGVVAAALGWSLSHRWAVLLLGAVAIGSSFFLFGRLGSAFMPDADPGELR
                     VSYKADPGISLDRSIEIARSVVEEVRKNPAVRYTYTTAGGTGGQPVNEGSIYVRLTEP
                     GTRPHFTIVKADFRERLKRFHGLRTSVGEAEENAAETKPVQISVRGADLRQVASISEE
                     LMAQMRQVPGAADIDTTEEQPRPEVRIRIDRKATGDLGLDLGGVASTVTGLVAGEVVS
                     QFEDPDGDSYDVRLRLNREERASNADLLAIDLPGHGGRELVPLSQIARLEDGAAPSKI
                     RRLDLMREVRVSANTENRSLGEVVGDITARSAAMKIPPGYTLDLTGEYEDMMESFGYV
                     LQSLFLAVILIYAILASQFQSFLQPLAIMFSLPLSLLGVAVMLYLAFDTLNMMSMIGV
                     ILLMGLVTKNAILVIDFANVQRRAGLSRREALISASTVRLRPILMTTLAMIFGMLPLA
                     FELGSGAEFRAPMARAVIGGLITSTLLTLVVVPVVYTYLDDGAGILAKWWGHKPREKS
                     RMPVTASTD"
     gene            complement(3881..5014)
                     /locus_tag="VFQ79_07645"
     CDS             complement(3881..5014)
                     /locus_tag="VFQ79_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0139566.1"
                     /translation="MRSLLLVAALAGAFAAAGCHREKPESVSAAEVRPVTVKTIPVTS
                     ETFTATIAVTGTLVSSSRVDVKAETTGRIVRFPKEQGDRVQAGESVAWVDDENYRLAL
                     RQSESAVQVAQAALHRAKVMEAHTEAELERSRNLVKSGGITERDLRAAEVAEKDARAQ
                     VALASAQLDQAVSAVEVARKRLRDAVILAPVSGVIQEKHVNAGAYVEPPTPVFSLVDN
                     SRLELRSPVPAAEIGPIRTGQQVLFSVSSFPGVEFSGRVIELAPAVDEQSRAAHVRIQ
                     IANPDGRLRAGMFADGQIITGARPNTVVIPGSAIYREDQSRKDSYVFVAVADKAVKRG
                     VRIGREKGPLVEIVEGLAPGDILIAERSVELAEGVAIQQSAHP"
     gene            complement(5017..6363)
                     /locus_tag="VFQ79_07650"
     CDS             complement(5017..6363)
                     /locus_tag="VFQ79_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0139567.1"
                     /translation="MAGRFYLPAVLVMVCVALSPAWPQSDDLHPLTLAEAVRIALEKH
                     PDIGKARAGADALKGRIREVRAQALPEISILSTATRWRDPSLLNASGLDNFPEELREA
                     LVPSPVNLFDYSVSVKQPVYTAGKVGTALRLASVEAEGSLAEIDRAQQDVALDVVKAF
                     YAVMWSGRYYELVEATQQQRQLHLEMARTRLKNGVATEVDVLRSQVAVANGMPELVRA
                     RNAIRQARSRLNYFLVRPLNHQTAVSGAFEEKLWEQQDLEALWKEAQRRRPELQRLSI
                     AQRSAAAQIDLARAESRLRLDFASTYGIMARMPENLVNPQFARWTVGLNFTLPVFDGF
                     RRSGLVWQATANERAARLERQKMEQQVRLALSQGLDDLAAARETVEASRAAVNEAERV
                     LSMTQDNYKYGAATTLDIVDAQTAVSLAGTNLLRGLHDYSVARAELKWTMGLPPWE"
     gene            complement(6451..7866)
                     /locus_tag="VFQ79_07655"
     CDS             complement(6451..7866)
                     /locus_tag="VFQ79_07655"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEU0139568.1"
                     /translation="MAFEIFETMHFRLPMRTLALLAAAVCGLPAADFRVGAAAVKITP
                     PMGAPMSGYYYNRAADGVHDDLFAKALVFEQDGAKAAVVACDLSGVPHPIVEQARQAI
                     EQKTGISGARVMISATHTHTGPVLLSGATRYNLEGEMLAIGKKYAADLPQLIAESVEK
                     ANAALAPARISAAKGREESLTFNRRFHMKDGSVGWNPGKLNPNIVRPAGPIDPDVPVV
                     YFDSPDGKPIATYVNYALHLDTVGGLEYSADYPYTLGRQLSAVKGPGMLTLFTIGCAG
                     NLNHIDVTTREPQKGHGEAARIGTVLAGEVLKTFKRLEHLPTAAPKVRSETVLLPLPA
                     FEPRELEWAKKITPLFGKPNAAPFLDFVKAFRVTDTAARDGKPREAEVQVIALGNDLA
                     FVGLPGEIFTELGVAIKYASPYRYTVVAELANGSVGYVPNRKAYPEGAYEVVSARCAE
                     GSGEMLVQSATKLLVSLRAEK"
     gene            complement(7898..>9753)
                     /locus_tag="VFQ79_07660"
     CDS             complement(7898..>9753)
                     /locus_tag="VFQ79_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEU0139569.1"
                     /translation="IHCSSGSMRTLGISAFYHDSAAALVEDGAIVAAAQEERFSRKKH
                     DARFPRNAIGYCLAESGVALEDIDYIVFYDKPFLKFERLLETYLAFAPSGYRSFKMAV
                     PLWIREKLFQKSLLASELKSFAPKFDASKRMLFTEHHLSHAASAFFPSPFEEAVVLTM
                     DGVGEWATTSVAIGRGNRLEIAKEIHFPHSLGLLYSAFTYFTGFRVNSGEYKLMGLAP
                     YGRPVYVQQILSNLIDLKPDGSFHLNLEWFDYCTGLTMTNGKFDVLFGGPARSPEEPI
                     KQRHMDLAASIQAVLNEVVLRLTRSLADETGIENLCLAGGVALNCVANGKVLRDGKFK
                     RIWIQPAAGDAGGALGAALAAYHIYKENPRQPAETDAMKGSYLGPAFRQEEIEARLQK
                     AGARFTTLNDECLVRTCAASLVQEKALGWFQGRMEFGPRALGARSILGDARSPSMQSV
                     LNLKVKYRESFRPFAPSVLAEDVSEWFDLNDPSPYMLLVADVARHHRRTMSPEEEGLF
                     GIEKLNVPRSDIPAVTHVDYSARIQTVHRETNPRYHQLLSAFKDTTGCGVLVNTSFNV
                     RGEPIVCTPEDAFRCFMGTEIETLAVGNCFLQKEDQDPALKQKYAHRLEAD"
BASE COUNT         1884 a         3045 c         2847 g         1977 t
ORIGIN      
        1 ctattccctt tgccgtggcg atctgttgga cctggtcgtt agcctgtgag tgatcttcca
       61 cgagcttttc gccgatttgc ttcacctcat cactggctgc cttctcctgc gccaactgtc
      121 ctaactggac ctcggccata ccgccttgtg cagctttgct gaggaacttg cgttcccccc
      181 tgctcagctt atctccagct gttgccgtgc gagcctgatc cgcacgttca ctctccgcgg
      241 tctgcggctg tgtctggggc acagtcgtct cttcttgcgg agcggtcctc tcctgttgcg
      301 gtagagttct ctcctggggt gcgggtgtcc tctgttgatc ggtctcttcc tgtgcgaaag
      361 cgaccggcat aatgaacagc aagcccgcgg cggcaagcgt tactgtcttt cccacgatct
      421 ttctcatacc tttgcctcct cggttcagag tcagacacta gtactgcacg taatagaccg
      481 taagtaccac gcacggctca gaatcggagt cttgcattta cagcaactta cagagccgcg
      541 accgttagaa gctgtgaaga aattgaattt agccacggat gaacacggat gaacacagat
      601 aagtttcctg ttttcaatct gtgtttatct gtgtccatct gtggctctga atctttcaca
      661 ccttctaagg gagcggggct gaacattttt gccctaacgg gagggcggca tggaaagaat
      721 tacgtggtgg agcgacggca agcaactagt cggtcgacgc agtaaccggc attctgcttt
      781 tttcgcgagg cttgtgtccc caccacttcg ctagaattcc cgccccatca tcaagatacg
      841 tatagacgac cggtaccacc acgagagtga gaagcgtgga ggtaatcagg ccgccgatga
      901 cggcgcgggc cataggtgcg cggaactcgg cgccggatcc cagttcaaac gccaggggaa
      961 gcatgccgaa gatcatcgcg agcgtcgtca tcaggatcgg ccgcagccgc acggtcgaag
     1021 ccgagatcaa agcctctctc cgcgacaagc cggcccggcg ctgcacattc gcgaaatcaa
     1081 tcacgaggat ggcgtttttg gtgaccagtc ccatcagcaa aatcacaccg atcatcgaca
     1141 tcatgttcag ggtgtcgaac gccagataca acatgaccgc cacgccaaga agcgacagcg
     1201 gcagtgagaa catgatggcg agcggctgaa ggaagctctg gaattgcgac gccagaatcg
     1261 catagatcag aatcaccgcc aaaaacagcg attgcaggac gtagccgaag ctctccatca
     1321 tgtcttcgta ttctccggtg aggtcgagcg tgtaccccgg cgggatcttc atggccgcgg
     1381 atcgtgccgt gatatctccc accacctccc cgagcgaacg gttttcggtg ttcgcgctca
     1441 cccggacttc gcgcatcagg tcgaggcggc ggattttcga tggcgcagcg ccatcttcca
     1501 gacgcgctat ctggctgagc ggcacaagct cacgcccgcc atgccccgga aggtcgatgg
     1561 ccaacaggtc ggcattgctt gcgcgctctt ccctgttcaa ccgcaggcgc acatcgtagc
     1621 tgtcaccgtc gggatcttcg aactgcgaga cgacctcgcc cgcaaccagc ccggttaccg
     1681 tggaggctac tcctcccagg tccaggccca aatcgccggt ggcctttcga tcgatacgta
     1741 tgcggacttc cgggcgcggc tgttcttccg tcgtgtcgat gtcggccgcg ccgggtacct
     1801 gccgcatttg ggccatcagt tcttcgctga tcgatgcaac ctgccgcagg tcggctccgc
     1861 gcacgctgat ctgaaccggc ttggtttccg cggcattctc ttcggcttcg cctaccgaag
     1921 tccgaagtcc atggaacctt ttcagccgtt cccggaagtc cgctttaaca atggtgaagt
     1981 gtggcctggt tcccggttcc gtcaatcgaa cgtagatgct accttcattc accggttgtc
     2041 cgcccgtgcc cccggcggtc gtgtaggtat agcgaaccgc ggggttcttt cgaacctcct
     2101 cgacgacgct tcgggcaatt tcaatcgagc ggtcaagact gatgcctgga tcggccttgt
     2161 aggaaacgcg caactcgccg gggtccgcat ccggcatgaa cgccgatccc agccgcccaa
     2221 acaggaaaaa gctggagccg atcgcgacag cgcccagcag cagcacggcc cagcggtgcg
     2281 acaacgacca tccgagcgcg gcggcgacca cgccacggag cttctcgatc tgtgcattca
     2341 acttgtcgag cgcacggccc accacggacc ttggaatcga cggatcggcg gcgggatcgt
     2401 accagcgcga agacagcatc gggtcgagcg taaagctgac gtacagcgag accagcacgg
     2461 cgaagcccac cgtaatgccg aattcataga agaactttcc gataattccg cccatgaacg
     2521 ctaccggcac gaagacggca acaatggtga aggtcgtcgc catgacggcc agcccgattt
     2581 ccgccgtggc ctcacgtgcg gcagtgaaat gatcggcgcc cgcgtgcatg tggcgcacga
     2641 tgttttcgcg cacgacaatg gcgtcgtcga tcaacaaccc gatggagaga gaaagaccca
     2701 tcagcgtcag gatgttcaag gtgaaaccga gcgcggccat gataatgaac gtgctgatga
     2761 ccgacaccgg aagcgccagc ccggtgatca cggtgctgcg ccagctattg aggaacagga
     2821 agacgatcag cactgtcagg gcggcgccga tgatcagggt cagctggaca tcttccaccg
     2881 actcgcggat aaagatggac cggtccacca cggtacgcag ccgtatgtgc gatgggaact
     2941 ccgcgttcat ctgcgcgacc gccgcttcca cgccatcggc gacgtcaacc gtgttcgcgc
     3001 cggattgctt cacgatatcg agcgccagcg cttgccggtc gtccaccagc gaggcgctgc
     3061 gccgttcttc aatcgagtcg acgatgcgcg cgacttcggg caggaaaacc ggtctgccgt
     3121 tgcgcgtagc cacaatcagg tcgcggaact cttccacctg ctggaatttg ccggatacgc
     3181 gcaccagcgg ctcgatctgg ccctggtcca gctttccggc ggggacgtcc agattctcac
     3241 gccgcagcgc cgctgaaact tccgcatagg taaggccgag cgccttcagc ttgttgcgat
     3301 ctacaaggat ctggatttcg cgccgggcca acccgatgag gtttacctgt ccgacaccgg
     3361 gcacgttctc caggcgccgc ttaatttgct tttcggcgat ggaggacagc gctttgatgt
     3421 ccatagtggg gctctcgacc gctatggaaa ccacaggcat cgcgttgaag tcgatgcgct
     3481 gcaccacggg ctgttccatc gacaacggaa agtcggcgcg aatcccgtca atcttcgcct
     3541 gtgtatcctg ctgcgcggtg tgtatgttcg tgccgagccg gaattcaacg acgattacgg
     3601 agaagccctc ggtggtggtg gaagagatgt gacggacgcc ggagatcgtg ttcaccgcct
     3661 cttcgatgcg cttggtcacc tcgcgctcca ccgtctcggg ggaagcgccg gggtagaggg
     3721 tctgaattgt cagcaccgga atctcaacat ccgggaacag atcgacgctc aactcgcggt
     3781 aggaataaat tcccagcgtc accagcgata cggcgagcat ggtggcgaaa acgggctgct
     3841 taatggagag atcgctgaga aacatcggtt ccccggaaac ttacggatgc gcggactgct
     3901 gaatcgcgac gccctccgcc agttcgacac ttcgctccgc gatgaggatg tccccgggcg
     3961 ccaggccttc cacaatttcg accagcggcc ctttctcgcg cccgatgcgc acgccccgct
     4021 ttacggcttt gtccgctacg gcgacgaaga cataggaatc tttccggctt tgatcctcgc
     4081 gatagatggc ggagcccggg atgactacgg tgttcggccg ggcaccggtg atgatctgcc
     4141 cgtcggcgaa catcccggcc cgaagacgcc catccggatt cgctatctga atgcgcacgt
     4201 gtgctgcacg ggattgctcg tccaccgccg gagcaagttc aatgacacgg ccggaaaact
     4261 ctaccccagg gaaactgctc accgaaaata gaacctgctg ccccgtccgg atgggtccga
     4321 tctccgctgc cggcaccggg ctccgcaatt cgagacggct gttgtccacc aggctgaaca
     4381 cgggcgtcgg cggttccaca taggctccgg cattcacatg cttttcctgg attacaccac
     4441 tgaccggcgc gagaatcacg gcatcccgca agcgcttgcg cgcgacttcc accgccgaga
     4501 cggcctggtc cagttgcgcc gacgccagcg cgacctgggc ccgcgcatcc ttctccgcta
     4561 cttcggccgc tctgagatct ctttcggtaa tgccgccgga cttcaccagg ttgcgcgaac
     4621 gttccagctc cgcctcggta tgggcctcca tcactttggc gcgatggaga gcggcctgcg
     4681 ctacctggac ggccgattcg ctctgccgca gcgccaggcg gtaattctcg tcgtccaccc
     4741 aggcgacgga ttctcctgcc tggacgcggt ctccctgctc ctttggaaag cgaacgatcc
     4801 gcccggtggt ttccgccttc acgtcgacgc gcgagctcga aaccagcgta cctgtcacag
     4861 cgatggtagc ggtgaacgtt tccgaagtaa ccggaatcgt tttcaccgtc accgggcgca
     4921 cttcggcggc gctgacgctt tcgggttttt ccctatggca tccggctgcg gcaaaggcgc
     4981 cggccaaagc cgccacaaga agaagactgc gcatacttat tcccacggag gtagtcccat
     5041 cgtccacttc agctccgctc gtgcgaccga atagtcgtgc agaccacgca gcaggttggt
     5101 tccagccagc gagaccgccg tctgtgcgtc cacgatgtca agcgtggtgg cggcgccgta
     5161 tttatagttg tcctgggtca tcgagagcac gcgctccgcc tcgttcaccg cggcacgcga
     5221 cgcttccacc gtctcccggg ccgccgcgag atcgtccaga ccctggctca aagcgagccg
     5281 gacctgctgc tccatcttct gccgttccaa gcgtgccgcg cgctcgttcg ccgtggcttg
     5341 ccacaccagc ccgctgcggc ggaagccatc gaacaccggc agagtaaaat tcaatcccac
     5401 cgtccagcgt gcgaactgcg ggttcacaag gttttccggc atgcgcgcca tgatgccgta
     5461 ggtgctggcg aaatccagac gcaacctgct ctccgcccgt gccagatcga tctgtgccgc
     5521 cgcgctgcgc tgggctatgc ttagccgctg aagctcgggc cgtctgcgct gggcttcttt
     5581 ccacagcgcc tccagatcct gttgttccca gagtttctct tcgaacgctc ccgagacagc
     5641 cgtctggtga ttgagcgggc gcaccaggaa gtaattcagc cgcgaacggg cctggcggat
     5701 ggcgttccgc gcgcgtacca gctccggcat cccgttggcc acggcgactt gagaccgcaa
     5761 cacgtcgacc tcggtcgcga caccgttctt gagccgcgtc cgggccatct ccaggtgcag
     5821 ttggcgctgt tgctgagttg cttcgaccag ctcgtaatat cgcccggacc acatcacggc
     5881 atagaatgct ttcaccacgt ccagggcgac atcctgctgc gcgcggtcga tttccgccag
     5941 ggagccctcc gcctccaccg aagcgagccg cagcgccgtt cccactttgc ccgcggtata
     6001 aaccggttgt ttcacgctga cgctgtagtc gaacaggttg accggcgaag gcaccagcgc
     6061 ctcgcgcagc tcctccggga agttgtccag gccggaggcg ttcaacaggc tggggtcgcg
     6121 ccagcgcgtc gcggtgctca ggatggagat ctccggcagc gcctgcgccc gcacttcccg
     6181 gatgcggccc ttcagagcgt cggcgccggc ccgcgccttt ccgatatcgg ggtgcttctc
     6241 cagcgcaatc cggaccgctt ccgccagggt cagcgggtga agatcatcgc tttggggcca
     6301 cgcaggggat agggcaacac acaccatcac caacacggcg ggtagataga aacggcctgc
     6361 catcacgcgt cacctgggga caatcaggac tgattctcca ttatgacacg gtcagtgaag
     6421 tttccatccg ggaatcgggt ggggcacccc ctatttttcg gcgcgaagcg ataccagtag
     6481 cttggtcgcg gattgaacca gcatttctcc cgacccttcg gcacaacgcg cgctcaccac
     6541 ttcgtaagca ccttccgggt acgcctttcg attcggcaca taaccaacgg atccgttcgc
     6601 gagttcggcc acaacggtat accggtacgg ggaagcatac ttgatcgcta cgccgagttc
     6661 cgtgaaaatt tcgcccggca ggcccacgaa cgcgaggtcg ttgccgagcg caatcacctg
     6721 tacctcagcc tcgcgcggtt ttccatcccg ggcggcagtg tccgttaccc ggaaagcctt
     6781 cacaaagtcc aggaagggag cggcattggg tttgccgaat agtggcgtga tcttctttgc
     6841 ccattcgagt tcccgcggtt cgaaagcagg caaaggcagc agcaccgtct cgctgcgcac
     6901 cttcggcgcg gcggtgggca ggtgttccag gcgcttgaac gttttcaaaa cttcgccggc
     6961 caggacggta ccgatccgcg cggcttcgcc gtgccctttt tgcggctcgc gagtggttac
     7021 gtcgatatgg ttcagattgc ccgcacagcc aatcgtgaag agggtcagca tcccggggcc
     7081 cttcaccgcg ctcaactggc gtccaagcgt gtacgggtaa tccgccgaat attccaggcc
     7141 gcccaccgta tccaggtgca aggcatagtt gacgtaagtc gcgatcggct tgccgtcagg
     7201 agaatcgaag tacactaccg gcacatcggg gtcgatgggg cctgcgggac ggacgatatt
     7261 cggattcaac tttccggggt tccaacccac gctgccgtct ttcatatgaa agcgccggtt
     7321 gaaagtaagg ctctcttcgc ggcctttcgc ggcggagatc cgggccggcg ccagcgccgc
     7381 attcgccttt tccacggact cggcgatgag ttgcggcaga tccgcggcgt acttctttcc
     7441 gatggcgagc atctcgcctt caaggttgta tcgggttgcg ccgctcagca aaaccggacc
     7501 cgtatgggtg tgggtcgcgc tgatcatgac acgcgcgcct gaaatgcccg tcttctgctc
     7561 gatcgcctgg cgcgcctgtt ccacaatcgg gtgcggcacg cccgagaggt cgcaagccac
     7621 cacggccgct ttggctccgt cctgctcaaa aaccagggcc ttggcaaaca ggtcatcgtg
     7681 tacgccgtcg gcggcccggt tgtagtagta gccggacatc ggcgcaccca tgggaggagt
     7741 aatttttaca gcggcggcgc caacccggaa atcggcagcc gggaggccgc atacggcggc
     7801 agcaagcagg gccagtgtgc gcatcggcag gcggaagtgc atggtttcga atatctcgaa
     7861 cgccacgatt cttgtcaaat gcgcgggcgg gagtgggtta gtccgcttcc aggcggtgcg
     7921 cgtacttctg tttgagagcg ggatcctggt cttctttttg aagaaagcag tttcccacgg
     7981 cgagcgtttc gatttcggtt cccataaaac agcggaaggc gtcttcgggc gtacagacga
     8041 tcggctcgcc gcgcacgttg aaacttgtgt tcacgagcac tccacagccg gtcgtatctt
     8101 tgaaagcgga cagcagttga tgatagcggg gattcgtttc ccgatgcacg gtttgtatac
     8161 gggccgagta atccacgtgt gtgaccgccg gtatgtcgga ccgcgggaca ttgagcttct
     8221 cgattccaaa gagaccttcc tcttcggggc tcatcgtgcg ccggtggtgt cttgcaacat
     8281 cggccaccag tagcatgtag gggctcggat cgttcagatc gaaccattcg gacacgtcct
     8341 cggcgagaac cgaaggcgcg aacgggcgga acgactcacg gtatttcact ttcagattca
     8401 ataccgactg catgctgggg gaccgggcat ctccgaggat ggaacgtgcc ccgagcgcgc
     8461 gcgggccgaa ctccatgcgt ccctggaacc atccgagagc cttctcttga accagtgacg
     8521 cggcgcaagt gcgaaccaga cattcgtcgt tcagtgtcgt gaacctggcg cccgcttttt
     8581 gcaaacgggc ttcgatctcc tcctgccgga acgcgggccc caggtatgat cctttcatcg
     8641 cgtcggtttc agccggttgt ctgggatttt ccttgtaaat gtgataagcc gcgagcgctg
     8701 cacccagcgc tccaccggca tcgccggccg ccggctgaat ccagatgcgt ttaaattttc
     8761 cgtcgcgcag caccttccca ttcgctacgc agttgagagc gactccgccg gcgagacaaa
     8821 gattttcgat tcccgtttcg tccgccagcg agcgcgtcaa ccggaggacc acttcattca
     8881 gaacggcctg gatggaggcc gccagatcca tgtggcgttg cttgatcggt tcctcggggc
     8941 tgcgtgccgg tccgccgaac aacacgtcga acttgccatt ggtcatggtg agaccggtgc
     9001 agtagtcaaa ccactccagg ttcagatgaa acgatccgtc aggcttcaaa tcgatgagat
     9061 tcgaaagaat ctgctgcacg tacacgggcc ttccgtacgg cgccaggccc atcagcttgt
     9121 attcgccgct attgacccgg aatccggtga agtaagtgaa cgcggagtag agcaatccga
     9181 gcgaatgtgg aaagtgaatc tctttggcga tttccagcct gttaccacgt ccaatggcga
     9241 cggaggtagt ggcccattca ccaactccgt ccatcgttaa caccactgct tcttcgaacg
     9301 gcgagggaaa gaaagcgctc gcggcatggc tgagatgatg ctcggtgaac agcatccgtt
     9361 tgctggcgtc gaacttcggc gcaaacgact tcagctcact cgccagcagg ctcttctgga
     9421 atagcttctc ccgtatccag agtggcacag ccatcttgaa ggagcggtag cccgaaggcg
     9481 cgaaagcgag ataggtttca agaaggcgtt cgaacttcag aaacggtttg tcatagaaga
     9541 cgatatagtc gatgtcttcc agagccacgc cgctctccgc caggcaatat ccaatggcgt
     9601 tgcgggggaa ccgcgcatca tgcttcttac ggctgaagcg ctcttcctgg gctgctgcca
     9661 cgatcgcgcc gtcttcaacg agtgccgccg cgctgtcgtg gtaaaacgca gagataccga
     9721 gcgtccgcat agaaccgcta gaacaatgta tag
//
LOCUS       DASTMK010000178         9503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_489292, whole genome shotgun sequence.
ACCESSION   DASTMK010000178 DASTMK010000000
VERSION     DASTMK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489292"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VFQ79_07665"
     CDS             <1..1045
                     /locus_tag="VFQ79_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0139570.1"
                     /translation="PLTGIMGMIDLLMETSVDEEQREYIRAARLCAETLNEILDSTLE
                     LAALSADQVVCERAEFHLAGLLHTAVMEYKAKAEAQGLELVYIRADGLPPVAIGDAIR
                     IRRIISILLNNAIKYTPRGRVEVRAQGERHHDRFHLSVAVADTGIGIAREQLDSIFES
                     FQQADTGLSRRYAGLGLGLSVAQKLVALMRGQITVESRLGEGSTFTLAIPLDLPGDAP
                     PAAPLAPGKKARILVVDDNSVVQQVVEHILKPGPYQVSGATSGTAALEALATTRFDAV
                     LMDLQMPDLDGFETTRRLRKIPGCATIPVLAFTANETDECRWLCRKAGMRGFVSKPVQ
                     REHLISALEECTE"
     gene            1216..1593
                     /locus_tag="VFQ79_07670"
     CDS             1216..1593
                     /locus_tag="VFQ79_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0139571.1"
                     /translation="MNGHEANRKRTILLVEDEASTRQFLQAVLTGSDFSVLESPNGME
                     ALRLMESHRPEIDLLLADVIMPQMGGRELAARLRVSNPNLKVLFISGYEHSLIGRLDT
                     GTDFLQKPFTVDKLRLKIKKLLG"
     gene            1639..2766
                     /locus_tag="VFQ79_07675"
     CDS             1639..2766
                     /locus_tag="VFQ79_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0139572.1"
                     /translation="MEAQERQSEPREQAFPGSVNVFSALGVPIRLHFTFLLLAIFLIV
                     LGLSGTQSAPMNVAYISALFLSVLLHELGHAAVAKRYGIRTLEIVMFPIGGLARLNRN
                     PKAREELWIALAGPLVNMLIAGVIFASLFRWGTILPWEQIVAASDGNLLQRIAVGNLI
                     LALFNMLPAYPMDGGRVLRSLLARFQSEDEATRMAARAGQVLAMLMGLIGLLYMQFFL
                     VFIALFVYLGASHEAAAVVGRSLTQGVPVKAAMVTDFRTLSHGDTIGDAANLLLSTSQ
                     QDFPVLHGGSVIGLLGRNSLVRSMAMEGPEAYVASAMNRNFTALAPDMDLAEALPKMG
                     QAGSCALVMDGAQLAGLLTAENISEFLVLRRIGMKPELRTV"
     gene            2793..4136
                     /locus_tag="VFQ79_07680"
     CDS             2793..4136
                     /locus_tag="VFQ79_07680"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0139573.1"
                     /translation="MHRVVIIGGGFGGLQAAKMLGRSPAQVVLIDRRNFHLFQPLLYQ
                     VATGGLSPGDIASPLRSILRKQKNTAVLMGTVVDVDADNRKVILENGELEYDSLVVAT
                     GCTHSYFGHPEWEISAPGLKTIEDATGIRRKILFAFEAAEREADPERRRALLTFVVVG
                     AGPTGVELAGSLGSIANDTLKDDFRSIRPQEARILLLDAAPRVLPTYPPELSEKAEKS
                     LIALGVRPMPNCTVIGVDDEGLDIKNGSGQGRIASHTVLWAAGVAASGLGRVLSERTG
                     ALLDKAGRVIVESDLTIPGRPEIFVIGDLAHFEQDGKPLPGVAPVAMQQGRYVGKLIT
                     ARLKGTRLPSFRYLNKGNLATIGRASAVADFGFVRFGGLFAWITWLFVHLMYLVGFQN
                     RLVVFLKWAYEYLTFNRGARLITGENPPLPEIVPPDPETCRSSRTNTPVSLDALR"
     gene            4404..4793
                     /locus_tag="VFQ79_07685"
     CDS             4404..4793
                     /locus_tag="VFQ79_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139574.1"
                     /translation="MSSVSVTGTDATIDASAREGGSIIFAGSGGHVHLSADLGIDVRI
                     SQQNYVGVFEATARGPVRVRLPHRFASCFEANVRSRAEFACRVELGSPLRFRRDHGRI
                     AVHHGSEDPPRMRLTSLEGAVVIDKSS"
     gene            4820..6097
                     /locus_tag="VFQ79_07690"
     CDS             4820..6097
                     /locus_tag="VFQ79_07690"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0139575.1"
                     /translation="MKHPKPLSRRRFIQTTAAAGAALGPVGRGQSTQQPRSGETEYFW
                     YREQPPGKYIDSQRGRKAFAYADGKVFLSTDNGHTWQHSAEFPDASKITFSHIMKNGN
                     ILFATGSKLYLSTDNLKGYREVIVKTPDGADYIPHTPKNPELPGWYFHTLPGVVSWEV
                     NGKEMMVWGNYCNVLGGATPVNIYYSTDSGRTVKIAYSFGQNPFFSDTGAPGGGKGGA
                     LIGNPDNKTIARHVHTVAYNPVENAFYATTGDGTRGFGHECHWLRGTYDARRDRWNWD
                     VIISDVSNSRYKCGGINFVDGKLYWISDSNGPPPYDRGIFVCDPKDIRNPEKHTRLFN
                     PEVESACMIIQDGTFLATHCAPASPLNTGFIVSNDMGKTWAQPDLKEFGKRSPVRLHE
                     KNDEGWFRVDLRSGWIQHAEVLFIKPKPTRRKA"
     gene            complement(6098..6523)
                     /locus_tag="VFQ79_07695"
     CDS             complement(6098..6523)
                     /locus_tag="VFQ79_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU0139576.1"
                     /translation="MTPVESQYFLAKTDPETYSLADLERERQTVWDGVRNPQALKAIR
                     DMKPGDRVFIYHSGGKAAVVGLAEVVSKPREDPRNPKLSVVDLRFVTSLDPPTTLVEI
                     KESGLFSDWALVRQGRLSTMSAPARFVDWMRERYPALQL"
     gene            6689..7348
                     /locus_tag="VFQ79_07700"
     CDS             6689..7348
                     /locus_tag="VFQ79_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEU0139577.1"
                     /translation="MKKSFACLLLFTISAVQAPAQQPSGGEEQKQRSEDASEVKEETT
                     GVKPTAEQLASGPNAPVDPKSFQLGPEDVLMVRVWNQPQISGIVSVRPDGKIGLPLIG
                     EIEAKGTTPHDLTQRITQALSTYYKDPLVMVTVQAVRSRKYYVTGQIAKGGMFPLVTP
                     TTVLQALSIAGGPSEWADLKKITILRNGKVLKFNYKDAIKGKNLDQNILLESGDHIIV
                     P"
     gene            7390..9099
                     /locus_tag="VFQ79_07705"
     CDS             7390..9099
                     /locus_tag="VFQ79_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139578.1"
                     /translation="MQPQAVESNYTDIVRRSLDIEDYIQIARRYKAWIIGPMFAGLVI
                     GVVVAFLLPNRYVSNAIIRVLPPPVPEKYVPPNVTSAISDRFTEMAQSVTSRSTLATL
                     VTTYDLYPQKRKRMPVEDIVEGMTKDLEIRPFQPLGQQGRRYEGAAAFRIAFTYENRF
                     LAQKVVTDVVGRIINEHLRMRASQSTMTTTFLREQWEQARKELDELEKKVTNFRVANQ
                     GRLPEQAQFNAQQMGSAQMRLSSLNSAISSVNQEKLVLEAQLRSFKQEAEALRRPPVA
                     AVAGGGEAFAIARNERIAELEKRIVSIESALNTMRQQLTENHPDIKTAQSTLASLKSR
                     RDEMVAEEESRLQKLAAEAKANPGRPAVVDPATMSKIRATDAAIEQVEAQIKARDLRL
                     QELSQEVQNVDRRMALYQTRMEATPVGEQEYTQLVREVALAKIRYEDLTRKKEQSETA
                     TDLENRNQGETMEILESASLPQTPVSPDRLAVVGIATALGCVLGIALAGTWEVKDTSL
                     KGLKDVRAYTNLPILGSVPLLENDVIIRRRKRMALLAWSTAVLIGIVIMSTSVYYHYA
                     TGV"
     gene            9118..>9503
                     /locus_tag="VFQ79_07710"
     CDS             9118..>9503
                     /locus_tag="VFQ79_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEU0139579.1"
                     /translation="MSRVHDALRRAEVSSSAPVQPPVLETPGQVSAVTPVVPTGSILD
                     NIKEIPFHPAPEAHLLNTTQPHEPPSEEFRTLRTKLNHIQTLQPIHSVVVTSPSPAEG
                     KSFAAANLALAESHLEGNRTLLADFD"
BASE COUNT         2033 a         2791 c         2809 g         1870 t
ORIGIN      
        1 gccactcacc ggaatcatgg gcatgatcga cttgttgatg gagacctccg tcgatgagga
       61 acaacgggaa tacattcggg cagcccgtct gtgcgcagag acgctgaacg agatcctgga
      121 ctccacgctc gaactcgcgg ctctctcggc agatcaagtg gtctgtgaac gggcggaatt
      181 tcatctcgcc gggctgttgc acacagccgt aatggaatac aaggctaaag cggaggcgca
      241 gggactcgaa ctcgtgtata tccgcgcgga cggccttccg ccggttgcga ttggcgacgc
      301 tatccggatt cgccgcatca tcagcatcct gctgaacaat gcgattaagt acacgccgcg
      361 cggccgggtc gaagtgagag cgcagggcga gaggcaccac gaccgcttcc acctgagcgt
      421 cgccgttgca gataccggaa tcgggattgc ccgggaacaa ctcgactcca tcttcgaatc
      481 cttccagcag gccgacacgg gcttgtcgcg ccggtacgcc ggcctcgggc ttggcttgtc
      541 ggtagcccag aagctggtgg cgttaatgcg ggggcagatc accgtggaga gccgcctggg
      601 cgagggcagc acgttcactt tggcaattcc gctggatctg cccggcgacg cgcccccggc
      661 ggcgccgctt gcaccgggaa agaaagcacg gatcctggtg gtagacgaca acagcgttgt
      721 gcaacaggtt gtggagcaca tattaaagcc tgggccctat caggtttcgg gcgccacctc
      781 cggcacagcg gcgctcgaag cactggcaac cacgcgattc gacgccgtgc tgatggacct
      841 gcaaatgcct gatctggatg ggtttgagac caccaggcgc cttcggaaga tacctggatg
      901 cgcaacgatc ccagttctgg cgttcacggc caacgaaacg gacgaatgcc gttggttgtg
      961 ccggaaagcg ggcatgcggg gcttcgtatc gaagccggtg cagcgggaac acctgatctc
     1021 ggcgctcgaa gagtgtaccg aatagtcata ttcaccggac cggtccgtaa caggtggggc
     1081 aaaccgcgag tctactttgc atggcggaac aagccgcttg aagtcacaag gttcggcgca
     1141 cacgttgtac gatgaaaatc caggcggcac gtcatgcggc ggacagatat cagacggtgt
     1201 ccggtctcct gaactatgaa cggccacgag gcaaatcgca aaaggaccat cttgctggtg
     1261 gaagatgagg cttccacccg gcaattcctc caggccgttc ttaccggatc tgatttttcc
     1321 gtgcttgaat cccccaacgg catggaagcc ttgcggctga tggaaagcca ccgcccggaa
     1381 atcgacttgc ttctggcaga cgtgattatg ccccaaatgg gcggcagaga gctcgccgcg
     1441 cgcctccgcg tatccaaccc aaatttgaaa gtattattca tttccggcta cgagcattcc
     1501 ctgattggcc ggctggacac cggcacagac tttcttcaga agcccttcac agtcgacaag
     1561 ctccggttga agatcaagaa actgctgggg tgattggcga atgcgctatg gtgaattgag
     1621 gagtggtccg ggagcggcat ggaagcacaa gaacgccaga gcgagccacg cgagcaggct
     1681 ttccctggct cggtgaacgt gttttccgcc ctgggtgtgc ctatacgcct tcactttacg
     1741 ttccttctgc tagccatttt tctgattgtg ctcgggttga gcgggaccca gtccgcgccg
     1801 atgaacgtcg cctacatctc cgccctgttc ctgtccgttc tattgcatga gctcggccac
     1861 gccgccgtag caaaacggta cggcatccgc acactcgaga tcgtcatgtt tccgattggc
     1921 ggcctggcac gcctcaaccg gaaccccaaa gccagagaag aactgtggat cgcgctcgcg
     1981 ggccctctgg taaatatgct gatcgccggc gtcatcttcg ccagtctgtt ccggtgggga
     2041 accattcttc catgggagca gatcgtcgcc gcctccgacg ggaatctgct gcagcgcatc
     2101 gccgtgggca acctcatact cgcgcttttc aacatgctgc cggcatatcc gatggatggc
     2161 ggaagggtgc tacggtcgct cctggcgcgg ttccaatccg aagacgaagc gacacgcatg
     2221 gcagcccgcg cggggcaggt gctggccatg ctgatgggat tgatcggcct gctctatatg
     2281 cagttctttc tggtgtttat cgcgctgttc gtgtacctgg gagcatcgca tgaggcggcc
     2341 gccgtagtgg gccggtcgct cacacagggt gttccggtaa aggcggccat ggtgacggat
     2401 ttccggaccc tctcgcatgg cgacaccatc ggggatgcgg cgaacctgct gctttccacc
     2461 tcgcagcagg actttcccgt cctgcacggc ggcagcgtga tcggactact cggccggaat
     2521 tccctggtgc gttccatggc gatggagggc ccggaggcct acgtcgcctc cgcgatgaac
     2581 cgcaatttca ccgcgctcgc tcccgatatg gatctggcgg aagcgttgcc taaaatgggg
     2641 caggcaggct cctgcgctct cgtcatggac ggcgcacagt tggccggatt gctgacggct
     2701 gagaatattt cggagtttct tgtgctccgg cgcatcggaa tgaaaccgga actccgaacc
     2761 gtctgacgtc tctcgatcat ccataaaatt ctatgcaccg ggttgtcatt attggcggag
     2821 gtttcggcgg acttcaagcc gccaagatgc tgggccgctc gccggcgcag gtggtgctga
     2881 tcgaccgccg caattttcat ctttttcaac ccttgcttta ccaggtggcg actggaggat
     2941 tgtcgcccgg ggacattgca tcgccccttc gctccattct gcgaaaacaa aaaaataccg
     3001 cggtattgat gggaacggtc gtcgatgtcg atgccgataa ccggaaggta atccttgaga
     3061 acggcgaatt ggaatacgat agcctggttg tcgcgaccgg atgcacgcat agctatttcg
     3121 ggcaccccga atgggagata tcggcgcccg gtctgaaaac catcgaagac gctaccggga
     3181 ttcgaagaaa aattcttttc gcattcgaag ccgccgaacg cgaagcggac ccggagaggc
     3241 gtcgtgcgtt gctcacgttt gtggtggtgg gcgccggtcc aacgggagtg gaactggcgg
     3301 gttcgctggg aagtatcgcc aacgacacgc taaaagacga tttccggtcg attcgtcctc
     3361 aggaagcgcg aatcctgctg ctggacgccg ctccgagagt gctgccaacc tatccgccgg
     3421 agttgtcgga aaaagcggag aagtcgctga ttgccttggg cgtccgcccc atgccgaact
     3481 gcacggtgat tggagtggac gatgaggggc tcgatataaa gaacggctcc ggccaggggc
     3541 gcatcgcctc gcacacggtg ctctgggccg ctggtgtggc tgcgtccggg ctcggacggg
     3601 ttctgagtga aaggaccggt gcgctcctgg ataaggccgg tagggtgatt gtagaaagcg
     3661 atttgacgat tcccggccgg ccggagatct ttgttatcgg cgatcttgcc cacttcgagc
     3721 aggatggcaa gccgctgccc ggggtcgctc cggtcgcaat gcagcagggg cgctatgtgg
     3781 gaaagctgat aacggcccgg ctgaagggca cgcggctgcc gtctttccgc tatttaaata
     3841 agggaaacct cgccaccatc ggccgcgcaa gcgcggtcgc cgatttcggc ttcgtccgat
     3901 tcggcgggct tttcgcctgg atcacctggc tgttcgtgca cctgatgtac ctggtcggct
     3961 ttcagaaccg gctggtggta ttcctgaagt gggcttatga atacctgaca ttcaatcggg
     4021 gtgcaagact catcaccggc gagaatccgc cgctacccga aattgtgcca ccggacccgg
     4081 aaacctgccg ttcttcccgc acgaataccc cggtctcgct tgacgctctt cgatgacgcc
     4141 ggcctcaaca gtttccaggg cggcggctac aatcagctat gagccttcgg tggataaatg
     4201 cagccttccg gcgcgttaac ctcgaagctg cgccggagtt cttgtttctc ccacagcgca
     4261 ttggaaggcc atttgatcct tcccttgtac caggccagtt tagattatcg gcttcccccg
     4321 gcccggtccg ggtttaccaa agagctgagc gcttccactt ttcgtcaaca aatctcgctg
     4381 ccttcaacct tcacgttggg ccaatgtcct ccgtgagtgt cactggcacg gacgccacca
     4441 tcgacgccag cgctcgggaa ggaggctcga tcatctttgc cggcagcggc ggccacgtgc
     4501 acttaagtgc cgatctcggg attgacgtca ggatttcgca gcagaactac gtcggcgtct
     4561 ttgaggcgac ggcacgcggc cccgtccgtg ttcgattgcc gcaccgcttt gcctcctgtt
     4621 tcgaagcaaa tgttcgtagc cgtgcggagt tcgcttgtcg agttgagctg ggatcgccgc
     4681 tgcggtttcg aagagatcat ggcaggattg ccgtgcatca tggcagtgaa gatcctccac
     4741 ggatgcggct gacgtctctc gaaggagcgg tggtcataga caaaagttcg tgatagcctc
     4801 tacagtcaga tatcgcacca tgaaacaccc gaaaccactc agcaggcgca gattcatcca
     4861 aaccaccgcg gccgcgggcg cggcgctcgg ccctgtgggc cggggacagt cgactcagca
     4921 accgagatcc ggcgagacgg aatatttctg gtaccgtgag cagcctcccg gcaagtacat
     4981 cgattcccag cgcggcagga aggctttcgc ctacgcggac ggcaaggtgt tcctgtctac
     5041 tgacaacggc cacacctggc agcacagtgc ggaattcccc gacgcctcta agatcacctt
     5101 cagccatatc atgaagaacg gcaacatcct cttcgccacc gggtcgaagc tctacctgag
     5161 cacggacaac ctgaagggct atcgtgaggt gattgtcaag actcccgatg gcgcggacta
     5221 catcccgcac acgcccaaga acccggaact ccccggctgg tatttccaca cgctccctgg
     5281 agtagtttcc tgggaagtca acggaaaaga gatgatggtc tggggcaact actgcaatgt
     5341 tctgggcggc gcgacgccgg tgaacattta ctactccacc gacagcggcc gtaccgtcaa
     5401 aatcgcgtac agctttggac agaacccgtt tttctccgac accggcgcac ccggcggagg
     5461 taagggcggc gcgcttatcg ggaatcccga caacaagacc atcgcccgcc acgtgcacac
     5521 ggtcgcctat aacccggtcg agaacgcctt ttatgccacc accggcgacg gcactcgcgg
     5581 attcggacat gaatgccatt ggctgcgggg aacttacgac gccaggcggg accgctggaa
     5641 ctgggacgtc atcatctccg acgtctcaaa ctcacgctac aaatgtggcg ggatcaactt
     5701 cgtggacggc aagctttact ggatcagcga ctccaacggt ccgccgccat acgaccgggg
     5761 aattttcgtc tgtgacccga aagatatccg aaaccccgag aagcacaccc gtcttttcaa
     5821 cccggaagtc gaatccgcct gcatgatcat tcaggacggc acctttctgg ccacgcactg
     5881 cgctccggct tcacccctga acaccggctt catcgtgtcc aacgacatgg gaaaaacctg
     5941 ggcacagccc gacctgaaag agttcggaaa acggtcgccg gtccggctcc atgagaagaa
     6001 cgacgaaggc tggttccgtg tggacctgcg gtccggctgg atccagcacg ccgaagttct
     6061 cttcatcaag cccaagccga cgcgccggaa ggcgtaatca gagttggagc gccggatagc
     6121 gttccctcat ccaatcgaca aaacgggcgg gcgcggacat cgtggaaaga cgtccctggc
     6181 gtaccagcgc ccaatcggaa aacaggccgg attctttgat ctcaaccaga gtcgtcggag
     6241 gatcgagaga cgttacaaac ctaaggtcga cgacgctcag cttggggttc cgcggatcct
     6301 cacgaggctt cgagaccacc tccgctaacc ccaccactgc cgcctttccg ccgctgtggt
     6361 agataaacac gcggtcgcca ggcttcatat ctctaatagc ttttagcgcc tgaggattgc
     6421 gaaccccatc ccaaaccgtc tgccgctccc gctcgaggtc ggcgagggag taggtctcgg
     6481 gatctgtttt ggccagaaaa tactgcgatt ccaccggcgt caccacctgt tccagtacta
     6541 gaaacgaaca gattcttgat gatagcgcat cctcaaaccc ggtctcagga ggaccggttg
     6601 gtgtaaaatg gctgctagtg ccgtattccg ggatgttaga gaaagggcga tcccacacgg
     6661 cgccaagttc cgtcgaggcc acatcacgat gaagaagtct tttgcctgcc tgcttctatt
     6721 cacgatatcc gctgtacagg cgcccgcaca acagccgtcc ggcggcgaag agcagaaaca
     6781 gcggagcgaa gatgcgagcg aggtaaaaga ggaaaccacc ggcgtgaagc ctaccgccga
     6841 gcaacttgca agtggaccga atgctcccgt cgatcccaag tcttttcaac ttggtccgga
     6901 ggatgtcctg atggtgcggg tctggaacca gccgcagatc tccgggatcg tctcggtccg
     6961 gcctgacggt aaaatcgggc ttcctcttat aggcgagatc gaagcgaaag gcaccacacc
     7021 ccacgacttg acacagagga tcacccaagc gctttccacc tattacaaag atccgctggt
     7081 aatggtcacc gtgcaagctg tccgcagcag gaagtattac gtcaccggcc agatcgcgaa
     7141 aggcggcatg ttcccgctgg ttacgcctac cactgtgctc caggccctca gtatcgccgg
     7201 ggggccgtcg gaatgggcag atctcaaaaa gatcaccatc ctgagaaacg gtaaggtatt
     7261 gaaattcaat tacaaagatg cgattaaagg caaaaacctc gatcagaaca tcctgctcga
     7321 gagcggcgac cacatcattg ttccctgaag ttcatgcagc aatcaggcga aagcaggcat
     7381 tgagggttca tgcaaccaca ggcggttgaa tcgaattata cggatatcgt acgccggagt
     7441 ctggacattg aggattacat ccagatcgcc cgccggtaca aggcgtggat tatcggtccg
     7501 atgttcgcgg gcctggtgat cggtgttgtt gtggcgtttc ttttacccaa ccggtacgtg
     7561 tcgaatgcta tcattcgagt gcttccgccg ccggtaccgg aaaagtacgt cccgccaaac
     7621 gtgacgtcgg ctatttccga ccggtttacg gagatggctc agtccgtcac cagccgaagc
     7681 acgcttgcca cgctggtgac cacttacgac ctttacccgc aaaagcgtaa gcgcatgccg
     7741 gtggaagata tcgtcgaggg aatgacgaag gacctcgaga tccgtccgtt tcagccgtta
     7801 ggccagcagg gccggcgtta cgagggtgct gccgcgttcc ggatcgcttt cacgtatgag
     7861 aatcgtttcc tggcgcagaa agtggtgacg gatgttgtcg gcaggatcat caacgagcac
     7921 ttgcgaatgc gggcgagcca gtccaccatg acgacgacat tccttcgcga gcagtgggag
     7981 caggcccgga aggaactgga cgagttggag aagaaggtta caaatttccg cgtggccaac
     8041 caggggcgcc tgccggaaca ggcccagttt aatgcacaac agatgggatc ggcgcagatg
     8101 cgcctgtcca gtttaaattc cgcgatctcc agcgtcaacc aggagaaact ggtgctggaa
     8161 gcgcagctcc ggtcgttcaa acaggaggcg gaagcgttgc ggcggcctcc ggtggctgct
     8221 gttgccgggg gcggcgaagc cttcgccatc gccagaaatg aacggattgc ggaactggaa
     8281 aaacgcatcg tctccatcga gagcgcgctg aacacgatgc ggcaacaact cactgaaaac
     8341 catcccgaca tcaaaaccgc gcagtcgacg ctcgcaagtc tgaagtcgcg gcgggacgag
     8401 atggtggctg aagaagagag ccggcttcag aaactcgccg cggaagccaa ggccaatccc
     8461 ggccggcctg cggttgtgga tcccgcaacc atgtcgaaga ttcgcgcgac ggatgccgcc
     8521 atcgagcagg tggaggcgca gatcaaagcg cgggatcttc gcttgcagga actcagccag
     8581 gaagtgcaga acgtggaccg ccggatggct ttgtatcaga cgcgcatgga agccacgccc
     8641 gtcggcgagc aggaatatac gcagttggtt cgcgaagtgg cgctcgccaa gatccgctac
     8701 gaggatttga ctcgcaaaaa agagcagtcg gaaaccgcta cggacctgga gaacagaaac
     8761 cagggcgaaa cgatggagat cctcgagagt gcatcactgc cacagacgcc tgtttcaccg
     8821 gatcggctcg cagtggtcgg gattgcaacg gcgctcggct gcgtgctcgg cattgctttg
     8881 gccggaacgt gggaggtcaa ggacacatcg ctcaaagggc tgaaagacgt gcgggcctat
     8941 acaaaccttc cgatcctcgg aagtgtaccc ctgctcgaaa acgacgtgat tattcgccgc
     9001 cgcaagagga tggcgctgct ggcgtggtcg acggcggttt tgattggaat cgtcattatg
     9061 tccacgtcgg tctactatca ttacgctact ggggtttgaa ggagaccagc tactaatatg
     9121 agccgagttc atgatgccct acgccgcgcg gaggtctctt ctagcgctcc tgtccaaccg
     9181 cccgtcctcg agactccagg gcaggtgtcg gcggtaactc cggtagttcc gaccggttcc
     9241 atcctggaca atatcaagga aatccccttc caccccgcgc cggaggccca cctgctgaat
     9301 accacgcaac cgcacgagcc gcccagcgag gagttccgca cgcttcgaac caagctgaac
     9361 cacatccaga cgctacagcc catccactcg gtcgtggtga ccagcccttc gccggcggaa
     9421 ggcaagtctt tcgccgcagc caacctcgca ttggcggaat cacatcttga aggaaaccgg
     9481 accttgctgg ctgactttga ttt
//
LOCUS       DASTMK010000179         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_233071, whole genome shotgun sequence.
ACCESSION   DASTMK010000179 DASTMK010000000
VERSION     DASTMK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233071"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VFQ79_07715"
     CDS             complement(<1..163)
                     /locus_tag="VFQ79_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0139580.1"
                     /translation="MAYFDLSGQTAVVTGAATGIGEAIARRLAKAGARVAIADIDAGA
                     AAQVAAAIGD"
     gene            complement(190..636)
                     /gene="rplI"
                     /locus_tag="VFQ79_07720"
     CDS             complement(190..636)
                     /gene="rplI"
                     /locus_tag="VFQ79_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU0139581.1"
                     /translation="MEVILREDIEKLGGRGQVVKVAAGYARNYLLPKRLAVEATAANK
                     KIVEQERQAHLRREAKEVSEAQDLAKMLAAVTVTILQKAGEQDQLFGSVTAKDIAEGL
                     EKQNFTIDRRKIHLDEPIKQLGEHKVPVRLHRDVTMDITVNVVREE"
     gene            complement(653..964)
                     /gene="rpsR"
                     /locus_tag="VFQ79_07725"
     CDS             complement(653..964)
                     /gene="rpsR"
                     /locus_tag="VFQ79_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU0139582.1"
                     /translation="MAEQPRSGGGRPIAASQRPTGGDKAIVTGKKQYFRRKKVCRFCV
                     EKIDDINYKDVRMLSAFISERGKITPRRISGVCTPHQRRLSEAIKQARNIALLPFASA
                     V"
     gene            complement(966..1394)
                     /gene="rpsF"
                     /locus_tag="VFQ79_07730"
     CDS             complement(966..1394)
                     /gene="rpsF"
                     /locus_tag="VFQ79_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU0139583.1"
                     /translation="MRIYEELFIVRPNATDEEVDPLIQQFTQVITADGGEVQKTEKWG
                     VRRLAYRVQKQTDGLYILLQFTATPNAVKELERRMRVSDLVLKFLTVRIDEKLKRIEK
                     RKKSREKRAQRKPAQPQSAPTMPTAEQVMASAPETEEKEA"
     gene            complement(1403..1990)
                     /gene="pth"
                     /locus_tag="VFQ79_07735"
     CDS             complement(1403..1990)
                     /gene="pth"
                     /locus_tag="VFQ79_07735"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU0139584.1"
                     /translation="MDKSPVVVVGLGNPGPEYEFTPHNLGFLVVDRIADRNTIRISRK
                     ESMALVGQGRIADTPVVLAKPQTFMNLSGSSVKPLLARLSLGAGDLVVVYDDLDLPWG
                     GMRIRPGGSAGGHHGVESLIRSLGTSDFARLRLGVDPGHPTADNVQFLLSPLRKERRK
                     ELDELLDHAAQAVESIIAEGVEKSMTKFNRRAQGS"
BASE COUNT          369 a          650 c          519 g          475 t
ORIGIN      
        1 catcgccaat agcggcagcc acttgcgccg ccgcaccggc atcgatatcc gcgatggcta
       61 ctcgcgctcc cgccttcgcg agcctgcgtg cgattgcttc tccaataccc gtagccgcgc
      121 ctgtcacgac ggcggtctgt ccactgagat cgaagtatgc catggagaaa cgtggatacg
      181 ctgtgcccgc tactcttccc gtacgacgtt gaccgtgata tccatggtca catcgcggtg
      241 gagccgcacc ggaaccttgt gctcgcccag ttgcttgatc ggctcatcca gatgaatctt
      301 ccggcggtcg atggtgaagt tctgtttctc gagcccttcg gcaatgtcct ttgccgtgac
      361 ggagccgaac aactgatcct gttcgccagc cttctgcaaa atggtgaccg tgaccgcagc
      421 gagcattttc gccaggtcct gcgcttcaga gacttctttg gcttcgcggc gtaaatgggc
      481 ctgccgctcc tgttcaacaa tcttcttatt cgccgccgta gcttcaacgg caaggcgttt
      541 gggcaacagg taattccggg cgtagcccgc cgccaccttg acgacctggc cgcgcccgcc
      601 gagcttttcg atatcttccc gcagaatgac ttccatcgta tttcctccgg ccctaaacgg
      661 cagacgcgaa cggaagcagg gcaatgtttc gcgcctgctt aatcgcctcg ctaagccgcc
      721 tttgatgcgg cgtacagacg ccggaaatcc ggcggggcgt gatctttccc cgttcggaga
      781 tgaacgcgga aagcattctg acgtctttgt aattgatatc gtcgatcttt tccacgcaga
      841 accggcagac cttcttacgc cggaagtact gcttctttcc cgtgacgatc gccttgtccc
      901 cgccggtggg ccgctgagag gccgcaattg gccttccgcc cccgcttctc ggttgttccg
      961 ccatgctatg cctccttctc ctcagtctcc ggtgcgcttg ccatcacttg ttccgctgtc
     1021 ggcatagtcg gcgcgctctg cggctgggca ggcttgcgct gcgccctctt ttcgcggctc
     1081 ttcttgcgct tttcaatgcg tttcagcttt tcgtcgatcc ggacggtcaa aaacttcagc
     1141 accagatcgg aaacgcgcat acgccgttcg agttccttga cggcattcgg cgtggccgtg
     1201 aactgcaaca ggatgtacaa tccatccgtc tgtttttgta cacggtaggc gagccggcga
     1261 acaccccatt tttcggtctt ctgcacttcg ccgccatcgg ccgtaatcac ctgcgtgaac
     1321 tgctggatca gcggatccac ttcttcgtcc gtggcatttg gccggacgat gaacaactcc
     1381 tcgtaaattc tcatccttgt tctcaggaac cttgggcgcg acgattgaat ttcgtcatgg
     1441 acttttcgac gccttcggcg attatggatt ccactgcttg agcagcatgg tcaagcaatt
     1501 cgtccagttc ctttctccgc tctttccgca ggggagagag caaaaactgc acattatccg
     1561 cggtaggatg gccaggatcc acacccagcc gaagccgggc aaaatcgctg gttcccaggc
     1621 ttcttatcaa cgactcgacg ccgtgatgcc cacccgcgga acctccgggg cggattcgca
     1681 tgccgcccca gggtagatca agatcgtcat aaacaaccac aagatcgccg gcccccaacg
     1741 acaacctcgc gagcagcggt ttcaccgacg agccgctgag attcatgaac gtctgcggct
     1801 tggcgagcac caccggtgta tccgctatcc tgccctggcc caccagggcc atactttctt
     1861 ttcgcgaaat ccgaatcgta ttccggtctg cgatcctgtc gaccaccaga aaacccaggt
     1921 tgtgcggagt gaattcgtac tccggaccgg gatttcccaa cccgacaaca acgaccggtg
     1981 acttgtccat ttactccccg atcgtccatc gta
//
LOCUS       DASTMK010000180         9419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_456734, whole genome shotgun sequence.
ACCESSION   DASTMK010000180 DASTMK010000000
VERSION     DASTMK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456734"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFQ79_07740"
     CDS             complement(<1..689)
                     /locus_tag="VFQ79_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139585.1"
                     /translation="MCLVFLTLTPLLSGEQHPGFSGMVTDSSGAAVADVKLELKNSAG
                     AVLAISRTNAEGHFVWHGIPSGVYQVRVTAAGFAARELAVNTAVTRETTITLEPESVY
                     TRVTVSSTRGAVDETTSSSYVAIVKDSEALQSRPLPTIGNVLEHEPGILVQQTTYAQV
                     SPFLRGFTGYHVLNLVDGIRFNNSTFRSGPNQYLAFVEPSQAQRLEALLGPTGAQYGS
                     DSLGGTINVVTL"
     gene            815..2149
                     /locus_tag="VFQ79_07745"
     CDS             815..2149
                     /locus_tag="VFQ79_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139586.1"
                     /translation="MSESVSRRRFLGAAASSAALPVTAQSYSRIVGANDRIQIGQIGC
                     GHRAVGHWRMLKLSAATDPNFDLRSVCDIWSVNREKAANHVAEMFGKRPRTYKYSEEM
                     LADPELDAVMIATGDHQHARILAEVVRAGKDCYCEKPMANTLEDAKLARDTVLRSKQV
                     VQMGSQWLSDPYQHKVREIVRSGKLGKIVAIDQSWNFNGPRWHVPSDSNVAAIREQDT
                     DWKRWLLGRRSRPFDPRAYFEFRIFKDFSGGITDQWYSHGSGLVHFYLDTFIPDDTVS
                     NGGIFAWHDVRENPDTFQCVSTFQEKQVLYTYSTTFGNGYGDHTIIRGTRGTLYSPGG
                     EGSPQWWYTPESRSAWRTNVVFNNPSRSKPEPVRVAEETELAPVNQSDDLKYHTDDWF
                     RCMRNRKTPNGSIETGFAHSVAVIMATRSYREGRKMYWDRTREQIVDRPVSG"
     gene            2202..3431
                     /locus_tag="VFQ79_07750"
     CDS             2202..3431
                     /locus_tag="VFQ79_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEU0139587.1"
                     /translation="MNASETKLVAASAIFLGTYAVLALGRLPGLRIDRTGAAIIGASL
                     MLAFGVLTVEEAYAAIDYKTIVLLFGMMIVVANLRLSGFFTFAAAAIIEHLHRPIALL
                     GAVVAVAGVFSAFFVNDTICLALTPLIAGLTRAAGLNPVPYLLAVAMASNIGSVATIT
                     GNPQNMMIGAFSGIPYREFSAALAPVAIFGMFICFGAIALLYRRELGAHAAVCPSRRI
                     VRVNRVMMWKSLVVSGGMILFFFLGWPVAQVALIGGAVLLITRRVKPQKVYHEIDWPL
                     LVLFIGLFIVVAGIEKALAAEGWLQQTSQFPLYRQGVLTTITAILSNLLGNVPAVLVL
                     RPVVAELPNPQHAWLIVAMASTLAGNLTLLGSVANLIVVERARRENIHISFRDYLKAG
                     LPLGTLSLAAGMLFLWD"
     gene            complement(3393..5057)
                     /locus_tag="VFQ79_07755"
     CDS             complement(3393..5057)
                     /locus_tag="VFQ79_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0139588.1"
                     /translation="MEELRSASGRWPRHIDSPTLARKVAVFSAMIVAWVVATFLALDI
                     HVSTVDLPRTIVLCSMVILVAAALASFTLRILVRPLSILQRGMKAVEQGRLETIEFSR
                     SGDEIEFLGRSFNGMIEALLASREQIREHQEHLERRIRERTEALEVAMERACAASQAK
                     SEFLANMSHELRTPMSGILGMIGIVLDSKLEPEQHEQLETAQRCALSLLGLLNDLLDL
                     SKIEAGRMTLERIPFDIQEEIGILTKTHTMAVRQKGVDFILEISPEMPRFVWGDPLRF
                     RQIVTNLLSNAVKFTSKGWVKCSVSVSQPLIEPAASIVVRVMDTGPGIPRDKIDHIFD
                     KFTQVDGSISRRFGGTGLGLAIVRSLVDMHHGDLRVDSEPGMGSTFTVTLPLEPAACA
                     SCSGPEDSSKMDGVSEHPAGKRILLVEDNPVNQKVIAATLRRAGYQVVVASDGEQAIA
                     ALDRDEFALVLMDVQMPVLDGLEATRQIRANPRWANLPVLATTAHAMSGDRERCLEAG
                     MNGYISKPVHPAHLLTTVNEYLNGTAAGSVRASSSIPKEQHSGSKR"
     gene            5203..6471
                     /gene="aroA"
                     /locus_tag="VFQ79_07760"
     CDS             5203..6471
                     /gene="aroA"
                     /locus_tag="VFQ79_07760"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU0139589.1"
                     /translation="MNSVSGVIRVPGDKSISHRYAMLASIAEGASRIANYSSGADCQS
                     TLTCIEALGIRVSRENGIVRIEGKGLDGLRQPEGDLDAGNSGSTIRMLSGILAAQPFT
                     TRISGDDSLCRRPMQRIMKPLLQMGARIEARDLQFPPLAIEGARLQPIEYHLPVASAQ
                     VKTCVLFAGLFADGRTAVYEPAQTRDHTELALEAFGVKVERGKGLAAVKGRARLVGRE
                     LTVPGDLSSAAFFLVAALALPHSRLVIEGVGLNPTRSALLDFLMGSGASIRVLNLTQT
                     AGEIVADIVVTTSAVRGGVIEKGLTAALIDEIPVLAVLGAVSEEGLTVRDASELRVKE
                     TDRIATVAENLRRMGIAVEVSEDGFQVPGRQKFQAAEVDSFGDHRIAMAFSVAALLAG
                     GECKVRNAGAAAVSYPEFFDTLRSISTGDK"
     gene            6625..>9419
                     /locus_tag="VFQ79_07765"
     CDS             6625..>9419
                     /locus_tag="VFQ79_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139590.1"
                     /translation="MNSLRLMPVFNRPIPLLLCLLLACLPAAAQYTTASFSGIVIDPS
                     GATLPDSKVTVRNVDTGLTQTVTTDTTGSFLFSRLPVGNYELRVEREGFSAYVQTGIT
                     LTVNQAATQTVTMRVGQLTESVTVEANADLVVTQTGTVSQLVDQKRVVELPLNGRMAQ
                     TLVFVSAGTVDLGRNGCRICGHGGVYPGEQTAGVNGAGMAQVNYQLDGAGHNDTYLNV
                     NVPFPNPDAVQEFNLQASNFTAEYGNAAGGVVNIVTRSGTNDIHGTLFEFLRNGKLNA
                     RNFFAPTQDTLKRNQFGGSVGGPIVKDKLFYFGTYQGTRVRTAPAGRVAFVPTEAERR
                     GDFSSLSQQIVDPVTREPFPNNQIPASRISPVANYFLRYIPLPNRGGRELNFVGTQSI
                     ETENQFMVKVDYNLSKHQISGRYFFTDFDKPAVIPEDNVLAASSAGNAVRVQNISLNH
                     TYTATPTLLINSTFGLNRQRGGSLSSAPFGFPDAGVRIASAGMSELKAPPELALSVTG
                     AFSIGTNHLGDFDRGDFTIRENVTKIHGSHEFHFGGEAVRLTNNIINTFRMDGNFSFN
                     GQLSGDGLADFMTGRSSTFIQGGGEFKNLKGTRWSLFAQDNWRATQKLTLNLGVRWDP
                     YFPYYDRSGRVVCFQPGVRSQRYPNAPVGMIYGGDNHDETCPVGGSDANVWNLSPRLG
                     FAYRLTGDGKTSLRGGFGYFYTPIQASAFNPFTNIAPFAPGFSFDAVDFQDPFGSVGV
                     ANPFPEQYGPRVPGPEVQFTVPTEIRATFAKDFRTPLLTSWNLTLERQVGNDWVFRGA
                     YIGNKGTYFFGAAESSREINPAIYIPGQSTNANTQARRLYQDFSRIGLYESSNNTNYH
                     SLQINAEKRFSMGLSVLANYTWSKKMDDYRWTTPDNRRFDYGLSSEDIPHNFKFSNVW
                     QVPQTAAHGVLGKILNG"
BASE COUNT         1936 a         2736 c         2792 g         1955 t
ORIGIN      
        1 agtgtaacga cgttgatggt gccgcccagc gaatcgcttc cgtattgcgc cccagtcggc
       61 cccagcaacg cctccaggcg ctgggcctgg ctcggttcaa cgaacgccag gtactggttt
      121 ggcccggagc ggaatgtcga gttgttgaac cggataccgt ccaccaggtt cagcacatga
      181 taaccggtga agccgcgcag gaagggagac acctgcgcat aagtggtctg ttgaacgagt
      241 attcctggct cgtgctccag cacattgccg attgtgggaa gaggcctcga ctgcaacgct
      301 tcagaatctt ttacgatcgc gacgtaagag gaagaagtcg tttcatccac ggcgccgcgc
      361 gtcgacgata ccgtcacgcg tgtatagacc gactccggtt cgagcgtgat ggtggtttcg
      421 cgtgtgaccg ccgtgttgac ggcaagttca cgggcggcga aaccagcagc cgtcacgcgc
      481 acctggtaga ccccggacgg aataccgtgc cagacgaagt gcccctccgc attcgtgcgc
      541 gagatagcca gaaccgcccc cgccgagttc ttcagttcca gtttcacgtc cgcaacggcc
      601 gcgccggaag agtccgtaac catccccgag aagccgggat gctgttcgcc cgaaagcagc
      661 ggcgtcaagg tcaaaaaaac gagacacaaa agagcaggaa gaggattcca ggacatatta
      721 gcgataattt agcagcatac gttcaatatc cgtttaaaaa cacgtttcaa aaagttacca
      781 tgaaatacgg agcaccacct tcaaggaggt acacatgtct gagagcgttt ccaggcggag
      841 atttctcgga gcagcggcaa gttcggcggc gctgcccgtc acggcacaat cgtacagccg
      901 catcgttggc gccaacgatc gcatccagat cgggcagata ggctgcgggc acagagcggt
      961 ggggcactgg cgcatgctga aactttccgc cgcaaccgat ccgaattttg acctccggag
     1021 tgtgtgcgat atctggtccg tgaatcgcga gaaagctgcg aatcacgtgg cggaaatgtt
     1081 cgggaagcgg cccaggacct acaagtattc cgaggagatg ctggcagacc cggaactgga
     1141 cgcggtgatg atcgccacgg gtgaccatca gcacgcgcga attcttgccg aggtggtgcg
     1201 ggcggggaag gattgctact gcgagaagcc gatggccaac acgctcgaag atgccaaact
     1261 ggcgcgcgat acggtccttc gaagcaaaca ggtggtccag atggggtcgc aatggctcag
     1321 cgatccgtat caacataaag tccgcgaaat cgtccgcagc gggaaactgg gaaaaatcgt
     1381 cgccatcgac cagagctgga acttcaacgg gccgcgctgg catgtgccgt cggattcgaa
     1441 cgtagcagcc attcgcgagc aggatacgga ttggaaaagg tggctgctcg gacgccggtc
     1501 gcgtccgttt gacccgcgtg cgtatttcga attccgcatc ttcaaagatt tctccggcgg
     1561 cattacggac caatggtaca gtcatggctc cgggctggtt cacttctatc tcgacacgtt
     1621 catccccgac gacactgtct cgaacggcgg cattttcgcc tggcatgatg ttcgcgagaa
     1681 tcccgacacg ttccagtgtg tgtccacgtt ccaggaaaag caggtgcttt atacatacag
     1741 caccacgttt ggaaacggct acggagatca caccatcatc cgcggaacgc gcggcacgct
     1801 gtattctccc ggcggcgagg ggagccccca atggtggtac acaccggaat cgagaagcgc
     1861 ctggcgcacg aatgttgtat tcaacaatcc gtcccggtcg aaacccgaac cggtgcgggt
     1921 ggcggaggaa acggaactgg cgcccgtgaa tcaatcggac gatttgaagt accacaccga
     1981 cgactggttc cgctgcatgc gaaaccgtaa aacgccgaac ggtagcatcg agaccgggtt
     2041 cgcgcactcc gtagcggtca tcatggctac ccgcagctac cgggaaggca ggaagatgta
     2101 ctgggaccgg acgagggagc aaatcgtcga ccgtcccgtg tccggttgaa ccgcccgcat
     2161 ttcgtttcga tggccggtca ggacttattc gccgtacacc cgtgaacgcc tcggaaacca
     2221 agctcgtcgc agcctcggcg atttttctcg gcacgtatgc cgtgctggcg ctggggcgat
     2281 tgcccggttt gcgtatagac cgtactggtg cggcgatcat tggcgcaagc ttgatgctcg
     2341 cctttggggt gttgaccgta gaggaggctt acgccgccat cgattacaag accatcgtcc
     2401 ttctctttgg gatgatgatt gtggtcgcga accttcgcct ttcaggtttt ttcacctttg
     2461 ccgcggccgc gattattgaa catctgcacc gccccatcgc gctgttggga gcggttgtcg
     2521 ccgtcgcggg tgtattttcc gcattcttcg tgaacgacac catctgcctg gcgctgacgc
     2581 cgctcatcgc cggattgaca cgtgccgccg gattgaatcc cgtgccgtat ctgctcgcgg
     2641 ttgccatggc ttcgaatatt ggcagcgtag ccaccatcac gggaaatcca caaaatatga
     2701 tgatcggcgc gttctcgggc attccttacc gcgagttttc cgcggcgttg gcgccggtcg
     2761 ccatattcgg gatgttcatc tgtttcggtg cgatcgcgct cttataccgc cgcgagttgg
     2821 gagcgcatgc cgcggtgtgc ccatcgcgca ggatcgtgcg cgtgaaccgc gtgatgatgt
     2881 ggaaatctct cgttgtgtcg ggcggcatga tcttgttttt ctttctgggg tggccggttg
     2941 cccaggtggc gctcatcggc ggcgcggtgc tgctcatcac ccgccgcgtg aagcctcaaa
     3001 aggtatacca tgagatcgat tggccgctgc ttgtgctgtt cataggcctg tttattgttg
     3061 ttgccggcat cgaaaaagcc ctggctgccg aaggctggct tcaacaaacg tcgcagttcc
     3121 ctctttacag gcagggtgtt ctcaccacga tcacggcgat tctttccaat cttcttggca
     3181 atgtcccggc ggtgctcgtc ctgcgcccgg tggttgcgga attgccaaac ccgcagcatg
     3241 cctggctcat tgtcgctatg gcatctactt tagccggtaa cctcacgctg ctcgggtccg
     3301 tggctaacct gattgttgtg gaacgcgcgc ggcgcgaaaa catccacata tccttccggg
     3361 attacctcaa ggccggcctt ccgttaggga ctttatctct tgctgccgga atgctgttcc
     3421 tttgggattg atgaagacgc tcttacgctg ccggcagccg tcccgttcaa atactcgtta
     3481 accgtggtca ggagatgtgc gggatgaacg ggcttggaaa tatacccgtt cataccggcc
     3541 tccaggcagc gttcgcgatc gccgctcatc gcgtgcgccg ttgtggcgag gaccggtaga
     3601 ttggcccaac gagggttggc ccggatttgc cgcgtcgctt caaggccgtc cagcacgggc
     3661 atctggacat ccatcagcac cagggcgaat tcgtcccggt ccagtgccgc gattgcctgc
     3721 tcgccatcgc tggccaccac aacctggtat cccgcgcgcc gcagagtggc tgcgatgacc
     3781 ttttgattga cgggattgtc ttccaccagc agaatgcgct tgccggccgg atgttcggat
     3841 acgccgtcca tcttcgagga atcttccggt ccggaacacg atgcacaggc agcgggttcc
     3901 agcggtagcg tcaccgtgaa ggtgctgccc atgccgggct cgctgtcgac acggagatcg
     3961 ccgtggtgca tatcgacgag gctgcgcacg atggccagac ccagtccggt acccccgaaa
     4021 cggcggctga tgctgccgtc tacctgagtg aacttatcga agatatgatc gatcttgtcc
     4081 cgggggatgc ccgggcccgt atccattacc cgcactacga tggaagccgc cggctctatg
     4141 agaggctggg aaaccgaaac gctgcacttc acccatcctt tcgacgtgaa cttgacggca
     4201 ttgctcaata gattcgtgac aatctgccgg aagcggagag gatcccccca cacgaacctg
     4261 ggcatttccg gcgagatctc caggatgaag tccactcctt tctgccggac ggccattgtg
     4321 tgcgtcttcg tgaggatgcc gatctcttcc tgaatgtcaa aggggatccg ctccagcgtc
     4381 atgcggccgg cttcgatttt ggagaggtcc agcaaatcat tcaatagccc gagcaaagag
     4441 agggcgcagc gctgcgcggt ttcgagttgc tcgtgctgct ccggctccaa cttgctgtcg
     4501 agcacgatgc caatcatccc caggatgccg ctcatcggtg tgcgcagctc atgcgacatg
     4561 ttggctaaaa actcgctttt agcctggctg gcggcgcatg cccgctccat tgcgacctcg
     4621 agagcttccg tccgttcccg tattctccgt tcaagatgtt cctggtgctc acggatttgt
     4681 tcccgcgagg ccagcagcgc ttcaatcatg ccattgaagc tgcggcccag aaactcgatt
     4741 tcatctccgc tgcggctaaa ctcgatagtc tccagccggc cctgctcaac cgctttcatt
     4801 cctcgctgca aaatggatag aggccgtacc aggattctta acgtgaaact cgcgagtgcg
     4861 gccgcaacca ggatgaccat cgaacagagc acgatcgttc tgggtaaatc gacagtgctt
     4921 acatggatat cgagagcaag aaaggtggcg acgacccagg ccacgatcat tgcggagaac
     4981 acagccactt tccgggcgag cgtcggtgaa tcgatgtggc gcggccatct cccagatgcg
     5041 gaacgcagtt cctccatatt caacttttcg gcatctcgcg atctgtcgaa gatcagggga
     5101 ttacactagt acctcggact ttccttctta tgctgttcgc tcgaacgagc acacgccgaa
     5161 gctagaatag gaattccagg aatggacata acagtttctc ccgtgaactc cgtatccggc
     5221 gtcatccgag tgccgggcga caaatcgata tctcatcggt acgccatgct ggcgagcatc
     5281 gccgaaggag caagccgcat tgcaaactat tcgagcggcg cagactgcca atccacgctc
     5341 acctgcattg aagccttggg aatccgggtg tcccgcgaaa acggaatcgt ccgtatcgag
     5401 ggtaaagggc tcgacggtct ccggcagccg gaaggcgatc tggacgccgg caattcgggc
     5461 tcgaccatac gaatgctgtc cggaattctc gcggcccagc cgttcacgac gcgcatttcg
     5521 ggagacgatt cgctttgccg ccgccccatg cagcggatta tgaagccact tctgcagatg
     5581 ggtgcgcgga tcgaagcgcg ggacctgcaa tttccgccgc tggcaatcga aggcgcgcgg
     5641 ctccagccca tcgagtacca cctgccggta gccagtgctc aagtgaagac ttgcgttctg
     5701 ttcgccgggc tattcgccga tggccgcacg gccgtgtacg agccggcaca aacccgcgac
     5761 cacacggaac tggcgctcga agcgttcggc gtgaaggtgg aaagaggaaa aggactggcg
     5821 gcggtcaaag gaagggcgcg cctggtgggc cgcgaactga cagttccggg cgatctctcc
     5881 tcggccgcct ttttccttgt agccgcactc gcgttgccgc attcgaggtt ggtgatcgaa
     5941 ggtgttggtc tgaatcccac tcgctccgcc ctgctcgatt ttctgatggg ctccggcgcc
     6001 tccatacgcg ttttgaacct gacgcaaacg gcaggtgaaa ttgtggcgga tatcgtggtg
     6061 accacgtcgg ctgtgcgggg cggcgtaata gaaaagggcc tgaccgccgc attgatcgac
     6121 gagataccgg tgctcgccgt tctcggcgcg gttagtgaag agggcctgac ggtgcgggat
     6181 gcgtccgaat tgcgcgtgaa agagacggat agaatcgcga ccgttgcgga aaacttgcgc
     6241 cgcatgggta ttgcggtcga agtgtctgaa gacggatttc aggttcccgg acgccagaaa
     6301 ttccaggcgg cagaggtgga ctcgttcggc gaccaccgga tcgccatggc cttttcggtg
     6361 gcggcattac tggccggcgg cgaatgcaag gtacgtaatg ccggcgcagc cgcggtgtct
     6421 tatcccgagt ttttcgacac gctccgcagc atcagcactg gagacaagta aattttcgat
     6481 tttatcgtgt taacttgccc ctaataaccc ggtatttaaa ctgccccgcc gattttatgc
     6541 ccttgacgtg cggaagccgc cgtacatatc atcaataaaa ccaactctta agtagcacag
     6601 acttggtttc atgggggcaa ctgaatgaat tccttgcgtc tgatgccggt tttcaaccgg
     6661 ccaattccac tgttgttgtg cctgctattg gcgtgcttgc cggccgcggc acaatatacg
     6721 acggcgagtt tcagcggcat cgtcattgac ccgagcgggg cgacgctgcc cgacagcaaa
     6781 gtaacggtac gaaacgtgga cacggggctt acgcagaccg tgacgaccga taccacggga
     6841 tcttttctgt tctcgcgcct tccggtcggc aactacgaac tgagagtgga gcgcgagggc
     6901 ttttccgcct atgtgcaaac cggcatcacg ctgacggtga accaggcggc gacacaaacg
     6961 gtcaccatgc gggtggggca attgacggaa agtgtgacgg tagaggcgaa tgccgatctt
     7021 gtcgtcacgc agaccggtac ggtcagccag cttgtagacc agaagcgcgt agtcgagttg
     7081 ccgctgaatg gccgtatggc tcaaacgctg gtgttcgtct cggccggcac ggtagacctc
     7141 ggccgcaacg gctgccgcat ttgcgggcac ggcggcgtct atccaggcga gcagacagcg
     7201 ggggtcaatg gcgccggcat ggctcaagtg aactatcaat tggacggagc gggccacaac
     7261 gacacttact tgaacgtcaa tgtgccgttt ccgaatcccg acgccgtaca ggagttcaac
     7321 cttcaggcga gcaacttcac cgccgaatac ggcaacgccg ccggtggtgt agtgaacatc
     7381 gtcacccgtt ccggaaccaa tgacattcac ggaacgctct tcgagtttct gcggaacggg
     7441 aaattaaacg cgcgaaattt cttcgcaccc acgcaggaca cgctaaagag aaaccagttt
     7501 ggcggcagcg ttggcgggcc gatcgtcaaa gataaactct tctatttcgg aacgtatcag
     7561 ggaacgcgcg tgcgaaccgc acccgccgga cgcgtggcgt ttgtgccgac ggaggccgag
     7621 cggcggggcg atttctcctc cctttcccag cagatcgtcg acccggttac aagggagccg
     7681 tttccgaaca accagattcc cgcgagccgc atcagcccgg tggcaaatta cttccttcgc
     7741 tacatccctc ttccgaaccg cggtgggcgg gagttgaatt ttgtcggcac gcaatcgatt
     7801 gaaaccgaga accagttcat ggtgaaggtg gactacaacc tgagcaaaca ccagatcagc
     7861 gggcggtatt tcttcacgga tttcgacaag ccggccgtca ttccggaaga caacgtgctc
     7921 gctgccagta gtgccggcaa tgccgtccgg gtgcagaata tctcgctgaa ccatacgtac
     7981 acggcgacgc cgacattgct tatcaacagc acgttcggcc tgaaccggca gcgcggcggg
     8041 tccttatcga gcgcgccgtt cggatttccg gatgccgggg tgagaatcgc gtctgccggc
     8101 atgtcggagc tgaaagcgcc gcccgagctt gccctttcgg ttacgggcgc attttcgatc
     8161 ggcaccaatc acctgggcga tttcgaccgg ggcgatttca cgatccgtga aaacgtgacc
     8221 aagatccacg ggtcgcatga gtttcacttt ggcggcgaag cggtgcggct gaccaacaat
     8281 atcatcaaca cattccgcat ggacggcaac ttcagcttca acggccagtt gtcgggtgac
     8341 ggactggccg acttcatgac cgggcgatct tcgacgttca tccagggcgg cggcgaattc
     8401 aaaaacctga agggtacgcg ctggagcctg ttcgcgcagg acaactggcg tgcgacccag
     8461 aaactgacgc tgaatttggg ggtgcggtgg gatccgtact tcccctatta cgaccgttcc
     8521 gggcgcgtgg tctgctttca acccggggtg aggtcgcagc gatatcccaa tgccccggta
     8581 ggcatgattt acggcggcga caatcacgac gagacatgtc ctgtgggcgg ttccgatgcg
     8641 aatgtctgga acctttcgcc ccggctggga tttgcctaca ggctcaccgg cgacggcaaa
     8701 accagtctgc gcggcggttt tggttacttc tatacaccga ttcaagccag cgcgttcaac
     8761 ccgttcacca atatcgctcc gtttgccccg ggcttctcgt ttgatgcggt agattttcag
     8821 gacccgttcg gcagcgtggg cgtggcgaac ccgtttccgg agcagtacgg accgcgagtg
     8881 cccgggccgg aagtgcagtt cacggtgccc acggagattc gcgccacctt cgcgaaagat
     8941 ttccgcacgc cgctgctgac aagctggaac ctgacgctgg agcggcaagt gggtaacgat
     9001 tgggtgttcc gcggcgctta tatcggcaac aaaggcacct atttctttgg agcggcggag
     9061 agctcgcggg agatcaatcc ggcgatctac atccctggac aatcgacgaa cgcgaacacg
     9121 caggcccggc gtctctacca ggatttcagc cggatcggtc tctacgaatc gagcaacaac
     9181 accaattacc attccctgca gatcaatgcg gagaagcgtt tctcgatggg gctctcggtg
     9241 ctggcgaact acacctggtc gaagaagatg gacgactacc ggtggaccac gcccgataac
     9301 cggcgcttcg actatggcct gtcgagcgag gatattccgc acaacttcaa gttttccaat
     9361 gtctggcagg taccgcagac cgcagcgcac ggcgtgctcg gaaagatctt gaacggttg
//
LOCUS       DASTMK010000181        13008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_41055, whole genome shotgun sequence.
ACCESSION   DASTMK010000181 DASTMK010000000
VERSION     DASTMK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13008
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41055"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFQ79_07770"
     CDS             complement(<1..360)
                     /locus_tag="VFQ79_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl-terminal protease"
                     /protein_id="HEU0139591.1"
                     /translation="MNSRIKFVVMTSSTCLVTLLLWGAVAGRSATTPEDTYRHLAVYS
                     EVLSRIKSDYVEEPDMKNVTVGALNGLLESVDPYASYLSPEQYKQYQKSKDTQKGEVG
                     LVLSKKFGYVGVVDAIPG"
     gene            complement(538..702)
                     /locus_tag="VFQ79_07775"
     CDS             complement(538..702)
                     /locus_tag="VFQ79_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139592.1"
                     /translation="MNESREDSMINEMRFQSSLLHLAENAGGADGGSAWAAEAQQVSQ
                     LVSRIGTERT"
     gene            complement(734..4735)
                     /locus_tag="VFQ79_07780"
     CDS             complement(734..4735)
                     /locus_tag="VFQ79_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEU0139593.1"
                     /translation="MRHAIAILTICSLAGALPCRAAEPVEAAVITSYPGAFQKALDAF
                     HARYGAGRLSLILTGSNVSCAALQRARAVYVEGGFWSAEMSACTDAVREMARRGAAFS
                     GTMAPLITANWRIPPDARLAEASRYLSAGGPENLLGFLVLMHNAAGGMPEIPVPPIKP
                     SAPSGIYYPGAPAHFATLDEYLAWRKSANHEAADGPRVGILFFKSALNYGATAHIDAL
                     VAALERRSLVPVPAFGWPLKSVESLLTPGGNAAVELMLLVDPVMPSLENAQFLEKHRL
                     HAMNLMTTSVPEKQWRESESGLPPGRLGIQVINAEQSGITEPILIAATASQSEGGGLR
                     PITAQIELAAARARRWINLRTKTNFEKTIALVYFNNPPGKGTLGASYLDLIPSLHNVV
                     SRLSREGYRVSGALPDEEKLKKLLLLSGRNIGEYAPGELDQLLKESHVTLLPATRYEE
                     WFRQLPEEFQRNVMRAWGPPRASRLMTVHSGDRTYFVIPGIRLGNLFLLPQPLRGELA
                     QADAKTHDRKTPPPHSYIAAYLWIQHQLKADAIVHFGRHGTLEWLPGKDVAQQESDPG
                     AVLTGDLPHAYYYLVDGGGEFLQAKRRSSAVIISHLTPLLASAGVPADYRALKGLIEN
                     YERNRETAPVVASEHARQAWTEAKRLKLDAQLKLTDDMEESIRMERLDSYLHEMEEQA
                     IPLGLYGIGKLPSKDVIREAVTAYLLSAATARTKAGVEKHAAAWAESLVAGLPPEPVD
                     DLYKDLVNDACEWLARLRESPEAELQSLVDVLNGRFVASGVSGDPLRAPGALPTGRNL
                     HDQDPRGFPSKAAWAAGQRMAGELVRQYRQKHGHLPKRVSFVLWYGESGRTQGLQEAQ
                     ALALLGVRPVWNGRGQVADVELIPPKELERPRVDVVLTMSGLYRDGMPEKLPLLDKAV
                     RLVREAPEDNAVQAHTVAVENDLIAAGIDPVKARQAARARLFGPQPGVFGVGMAGMME
                     FSRDAGDSHAAANLYLRNMNYAYGAGLNGEAVPGALKQQLKRNEVVVHGRSSNLYGLL
                     DNDETYQFAGGLNAATRAASGRVPEFLIANARKAGSERYDEANHFVRRELATRLENEK
                     WIEGMKSAGYAGAQQISKEIEHLYGFRATAPEQVDPEVWQQLLDTYIRDKRNLGLEKW
                     FRESNPHARQMVAARLIEIDRQGVYRYSAEDRRSLVSAYIKSVNESGVSCYANACGNR
                     KLIRHVAQVARETGGMDAKDVQRYEIALQRTATPATANLTSHNAKRGPPVRMSGASPL
                     RDMFNGVRIFDAGPIQLRAPETALGWIMLLLPVPFGIAIGIVRRRIGQRPSMGLLCYV
                     QPRQVQAYAVKER"
     gene            complement(4754..4972)
                     /locus_tag="VFQ79_07785"
     CDS             complement(4754..4972)
                     /locus_tag="VFQ79_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139594.1"
                     /translation="MTSPIYRGVTSGQRVYMDGFEQFGFNTQRDLTSRIALRCTLRNL
                     TGNVDPAYRLTSPFGAEVSLRFKLGSVE"
     gene            complement(5110..6909)
                     /locus_tag="VFQ79_07790"
     CDS             complement(5110..6909)
                     /locus_tag="VFQ79_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139595.1"
                     /translation="MRKESFFIQIIVLGLLLLPAPAWSQRLCIAVKDPQGLPVNAAAI
                     RVEPLATSAVLRGVTGEDGEWCADTGAGVYVVRAFGSGMEGAAASVTVEPRGEQRLSL
                     ALRLSTVASQIEVTASRLPESLLDAPLPVRQVDAGAITQIGARQLTDALQEQPEVVTF
                     AGGTMSGGGSTNVQGFASRDVEILVDGEPLTGRVSGYVDLNQFDASMFESVEIKTGAS
                     AMTYGQQGMGGAINLITKRAGTGSHASVETGYGSFNTGLLRADGGYAKDGFAIYAAGA
                     AQRSLGYDLESDILGKTQSANRMRNLFSSLYLPQWKRLNTGLTFLWTDQNFWGFEGST
                     VTGIYDFERPKRRLVFLPRASLLLGSDSLLTVRGRKLYYRSDENLAYRDPLSLRPQST
                     HNEANGGDVEWSLARSRGFRLSTGLFFNRQSMVGDRINTEGNRAETDVWSQVTTAEIP
                     LVRSLRLMAGYRADHDTNFGGRLSPQAALAWKPFQRLSLSASVTRGMRAPDFNELYIL
                     HTHGGGRVRILGDPNLRPQKSWSYSASALTTVGSRTRVEARLFDHDMTDLIQTSLIGR
                     EGAASVYLYGNVGQARIRGGSTSYRMRRIANWN"
     gene            complement(7519..9468)
                     /locus_tag="VFQ79_07795"
     CDS             complement(7519..9468)
                     /locus_tag="VFQ79_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0139596.1"
                     /translation="MNCVKKCLSIACAFLLMAPWTAAQQPRANGSVLDEGGAWHSSLT
                     DPYRGKEIPPINLANSSRLDALIRAGRIYLSLQDAIALAIENSLDIEVARYGPRLAET
                     DLRRAQAGGSIRGVSTTVSQGASSATGSGTRGADLTGASLAQGGVGGSGGIGGSGTTG
                     GVPSLDPSFTSTISWGHRTQPQVNSFLVGANNVVSTNKNFNFELSQGFLTGTRVSLGM
                     TNQDQSTSSGRFDFNPYTTSALNLTLTQPLLQGFGLALNSRFIHIAKNNVQVSDLVFR
                     RQLIATVASVINLYWDLVAFNEDVAVRQQALKLAEKLLSDNRKQVEIGTLAPIEIIRA
                     EAEVAARQQDLTVAETNVLQQETILKDALSRVGVAGPLLAEARIVPTDHIRIPDVEAI
                     EPIQDLVASAMEKRPELSETRFQLENSRIGLRASKNALLPRLDGFIQLQNNALAGQVN
                     TIPVPSQTGGAPVIRDPDAIDPFFRGGFGTAVGQLFRRNFPDYSFGVQMTIPLRNRSA
                     QADVIANQLTLRQNELRQQQQVNAIRVEVQNALIGIQQARARYQTAVKNRVLQEQTLD
                     AEQKKYALGASTIFQIIQSQRDLALAKANEVTAMSQYARSRVEMDRATGRTLEINNVL
                     IEDAVRGEVSTRPSPIPVIDPQQNK"
     gene            9607..10206
                     /gene="pyrE"
                     /locus_tag="VFQ79_07800"
     CDS             9607..10206
                     /gene="pyrE"
                     /locus_tag="VFQ79_07800"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU0139597.1"
                     /translation="MIPTSEVLELFKQTGAYLKGHFRLTSGLHSPEYMQCALVLQYPE
                     YAERLGRELARTLRSLVGESAIDVVASPAIGGLIIGHEVARALGARHIFAERDASGRM
                     ILRRGLSAASGERAVVVEDVITTGGSSREVIQLLRESGANVVAAASIVDRSGGRANLE
                     APHTALATLGVTSYPPGECPLCRQGLPVEKPGSRPVSSQ"
     gene            10203..10952
                     /gene="truA"
                     /locus_tag="VFQ79_07805"
     CDS             10203..10952
                     /gene="truA"
                     /locus_tag="VFQ79_07805"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU0139598.1"
                     /translation="MRRIRLTLSYDGTGFYGWQKQPGLATIQGTLEQAVSEIEGAPVH
                     VAGSGRTDAGVHAVAQVAAISIENPIPLPNLKKALNRLLPCGIRVTAVDDVPPDFHPR
                     YHALAKTYEYRIVRTEVCSPFERHYAYHHPYPLDTARMLELAPMLEGEHDFTAFAASD
                     ERDALGLSKIRTIFRSELEEVDDRLIYRVRGSGFLKHMVRNIVGTLLEAGKGNLDSEG
                     LRTLLRSRGRTGCGPTAPARGLFLISVEYQM"
     gene            complement(10943..11389)
                     /locus_tag="VFQ79_07810"
     CDS             complement(10943..11389)
                     /locus_tag="VFQ79_07810"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0139599.1"
                     /translation="MKDKRRTVLKAAIGMAAGAGLAKAQSGPQKKVHYRGGKKPQGTP
                     LFSGAVSYGNLLFISGKGAHFEGDIKAHTKHVLDEVQKELENAGSSMNKVLKCNVYLN
                     DLKDYKEMNEVFRGRFGDEPPVRTTIAAAGGIPGDSLVEIDVIAYI"
     gene            complement(11431..11679)
                     /locus_tag="VFQ79_07815"
     CDS             complement(11431..11679)
                     /locus_tag="VFQ79_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139600.1"
                     /translation="MQTLSLVWGILALVGMLVGMFPCLGALNWLNIPFALAGLVVSII
                     ALATAGPNSRTGSIAGTICCAIATGLGLLRLAIGGGVL"
BASE COUNT         2599 a         3988 c         3727 g         2694 t
ORIGIN      
        1 acccggaatg gcgtccacca cgcctacata tccgaacttt ttggagagga caagccccac
       61 ctcgcccttc tgggtatcct tggacttctg atattgcttg tactgctcgg gactgagata
      121 gctggcgtag ggatcgacac tttccaggag accgttcagc gccccaaccg ttacgttctt
      181 catatccggc tcttccacgt agtcgctctt aatgcgcgac aaaacctctg agtagacggc
      241 cagatgacgg taggtatctt cgggggtggt tgcgctgcgg ccggcgacgg ctccccaaag
      301 gagcaacgtc accagacacg tcgatgacgt catcactaca aacttgatac ggctgttcat
      361 aaacggtgag ccttgtcagg tggaacttgc tgcacccatt ataacagaca cttcaaatcg
      421 ctaaagtgca acacaatcct tataaagccc gttagtacag ccgtaactat aacttacgtt
      481 gggtggctgg accgcggcgc cgcgcgcgga tcgaagtaaa atcagcccgt gccgaggtca
      541 ggttcgttcg gtcccgattc gagaaaccaa ctgcgacacc tgttgtgctt cggcggccca
      601 ggccgaaccg ccatcggccc cgcccgcgtt ttcagccagg tgcagcaagc tggactggaa
      661 tcgcatttcg ttaatcattg agtcttcacg cgattcgttc atagcagctc tatcggacat
      721 ttcaggagaa tctttagcgc tctttgacag catacgcttg aacctgccgt ggctgcacat
      781 agcacaggag ccccattgaa ggtctctggc cgattcgacg ccgcacgata ccgatggcga
      841 ttccgaatgg caccggcagg agcagcatga tccagcccag cgccgtctcg ggggcacgga
      901 gttggatggg gccagcgtcg aaaattcgca cgccgttgaa catgtcacgc agagggctcg
      961 ccccgctcat tcgcacggga gggccgcgtt tggcgttgtg cgaagtgagg ttcgcggtgg
     1021 caggcgttgc cgttctttgg agtgcgatct cataccgttg cacgtccttg gcatccatgc
     1081 cgccggtctc acgagccact tgcgcaacat gccggatcag cttccgattc ccacaggcat
     1141 tcgcgtaaca gctcacccca ctctcattca cggatttgat gtacgcggag acgagcgagc
     1201 gcctgtcttc cgcgctatac cggtacacgc cctggcgatc gatctcgatt agtcgggccg
     1261 cgaccatctg gcgcgcatgg ggattactct cgcggaacca cttctccagc cccaggttcc
     1321 gcttgtcgcg aatgtaggtg tccaaaagtt gctgccacac ttccggatcc acttgctccg
     1381 gcgccgtggc gcggaagccg taaagatgct cgatctcctt ggagatctgc tgcgccccgg
     1441 catagccggc ggatttcatg ccctcaatcc acttctcgtt ctccaggcgc gtggcgagtt
     1501 ctctccggac gaagtgattc gcctcgtcgt atcgttcgct tccagccttg cgcgcgttgg
     1561 cgatgagaaa ttcgggaacg cgtccggaag cggccctggt cgcggcattc aggcccccgg
     1621 cgaactggta ggtttcatcg ttgtccagca atccgtacaa gttggacgaa cgcccgtgga
     1681 cgaccacttc atttcgcttg agctgctgct tcagcgcccc cggcacggct tcgccattga
     1741 gtccggcgcc gtaagcgtag ttcatgttcc tcaaatagag gttggccgcg gcatgcgagt
     1801 cgccggcgtc gcgcgagaac tccatcatgc ctgccattcc gactccgaat acccccggct
     1861 gtggtccgaa caagcgcgcc cgtgcggctt gccgcgcctt tactggatcg atgccggcag
     1921 caatcaagtc gttctccaca gctactgtgt gcgcctgtac cgcgttgtct tccggcgcct
     1981 ctcgcaccag ccgcacggcc ttgtccagca gaggaagctt ctcgggcatc ccatcgcgat
     2041 agagtccgga catcgtcagc acgacatcga ctcgtggccg ttcgagttcc ttcggcggaa
     2101 tcaactcgac atcggcgacc tggcctcgtc cgttccacac cggccggaca cccagcaggg
     2161 ccaatgcctg tgcctcctgc agtccttgtg ttcgcccgct ttcgccatac cagagcacaa
     2221 acgacacacg cttgggcagg tgcccatgct tctgccggta ctgacggacg agttctcccg
     2281 ccatgcgctg gcctgcagcc catgccgcct tggacgggaa tcctctcgga tcctggtcgt
     2341 gcagatttct ccccgtcggc aaggctccag gcgcgcgcag cggatcgccg gatacaccac
     2401 ttgctacgaa ccggccgttc agcacgtcca ccaggctctg cagttccgct tcgggcgact
     2461 cacgcagccg cgccaaccat tcgcacgcat cgttgacaag gtccttgtac aagtcgtcga
     2521 cgggctccgg cggcaatcca gctaccagac tttccgccca ggcggcggca tgtttctcca
     2581 cgccggcctt tgtccgcgct gtcgccgcac ttagcaagta agcggtcacg gcttcacgaa
     2641 tgacgtcctt cgagggaagc ttgccgattc cgtacagccc aagaggaata gcctgctcct
     2701 ccatctcgtg aagataggaa tcgaggcgtt ccatccggat ggattcctcc atgtcatcag
     2761 tgagcttgag ttgtgcatcc aacttgagcc gcttggcctc cgtccaggcc tggcgagcgt
     2821 gttcagatgc gaccacaggc gcagtttccc gattccgctc gtagttctcg atcaacccct
     2881 tgagtgcccg atagtccgcg gggacaccgg cggacgccag caaaggtgtg agatggctga
     2941 taatgacggc gctcgaccgg cgtttggcct gtaggaactc gcctcctcca tccaccaggt
     3001 agtaataggc gtgtggaaga tcgccggtca acacggcgcc gggatcgctc tcctgttgtg
     3061 caacgtcctt gccgggcagc cactccaggg tgccgtgacg gccgaaatga acgatggcgt
     3121 cggccttcag ttggtgctgg atccaaaggt acgcggcgat ataggaatgg ggcggcgggg
     3181 tcttccggtc atgagtcttc gcgtccgcct gcgccagttc tccccgtaag ggttgaggga
     3241 ggagaaacaa gttgccgaga cgaatccccg ggatgacaaa atacgtcctg tccccggagt
     3301 gaacggtcat cagccgggac gcgcgcggcg ggccccatgc ccgcatcacg ttccgttgaa
     3361 actcctcagg caattgccgg aaccactcct cgtagcgtgt cgccgggagc aaggtcacgt
     3421 ggctctcttt gagcagttga tccaactcgc cgggtgcgta ttcaccgatg ttgcggccac
     3481 tcagcaacag cagctttttg agcttttcct cgtccggaag cgctccgctc acccgatagc
     3541 cctcgcggct cagccgcgat acgacgttgt gcagactggg gatcaggtca agatagctgg
     3601 cacccagcgt gcctttaccc ggcgggttgt tgaagtagac cagcgcgatt gtcttctcga
     3661 agttcgtttt ggtgcgcagg ttgatccagc gccgtgcccg ggccgccgcc agttcgatct
     3721 gcgcggtaat cggccgcaga ccgccacctt cggattgcga tgccgtagcg gcgatcagga
     3781 tcggctcggt gataccggac tgctcggcat tgatcacttg aatccccagc cgccccggag
     3841 gcaaaccact ctccgattcc cgccactgct tttccgggac agacgtggtc atcagattca
     3901 tggcgtgcag ccggtgtttt tccaggaact gcgcgttctc cagactgggc atcaccggat
     3961 ccacgagcag catgagttcg actgccgcgt tgccccctgg agtaagcagc gattccaccg
     4021 acttcaaggg ccatccaaac gccggcaccg gaacgaggct gcgccgttcc agcgcggcca
     4081 ccagggcatc gatatgtgca gtcgctccat agttcagcgc ggatttgaag aagaggattc
     4141 ccactcttgg accgtccgcc gcttcgtggt tggcgctttt tcgccaggcc agatattcat
     4201 cgagcgtggc gaagtgcgcc ggcgctccgg gatagtagat tccagaggga gcgctcggct
     4261 tgatgggtgg cacggggatc tccggcattc cgcccgcggc gttgtgcatg agaaccagaa
     4321 accccaacag gttctccggt cctcctgcag acagatatcg tgatgcttcg gcaagacgtg
     4381 cgtcaggcgg tatgcgccaa ttggccgtga tcaacggagc catcgtgccg ctgaaagccg
     4441 cgccgcgccg ggccatctcg cggacggcat ccgtgcaggc cgacatttcg gcggaccaga
     4501 atcctccctc gacgtacact gccctggctc gctggagcgc cgcacacgag acattggagc
     4561 cggtcagaat cagcgacagg cgtcccgctc cgtaccgggc atgaaaagcg tcgagtgcct
     4621 tttgaaacgc ccccggataa gaagtaatga cggccgcttc aaccggttcg gccgcccggc
     4681 acggcagcgc cccggcgagt gaacaaatcg tgagaatcgc tatggcgtgt ctcatgcatt
     4741 tctctcccgg cggttattcc acgctgccca gcttgaaccg cagggaaacc tcggcgccga
     4801 agggagaagt gagacggtaa gctgggtcga cgttcccggt caggttgcgc agcgtgcacc
     4861 gtagagcgat ccggctggtc aaatccctct gagtgttgaa gccgaactgc tcgaagccgt
     4921 ccatatagac gcgctgtccg gaagtgacgc ccctataaat ggggcttgtc atattgccaa
     4981 acaggctgac cagaagacca tgacgagggg acgcccaggt tgcccgcagg ctgccgcgat
     5041 gacggggtgc atcttccagc gtcgtccact cttgaagatt gagcgtgtcc aggtattgat
     5101 acgaccctgt cagttccagt tggcgatacg gcgcatacga tatgaggtgc tgccgccacg
     5161 gatacgtgcc tggccgacat tgccatatag gtaaacactg gccgccccct ctcgtccgat
     5221 gagactcgtc tgaatcaggt ccgtcatatc gtgatcgaac agccgggctt caacgcgcgt
     5281 cctggacccg actgtcgtca gcgcactcgc ggaataggac catgacttct gcggccggag
     5341 attcggatct ccgagaattc gcacacgccc tccgccatgg gtatggagaa tatagagttc
     5401 gttgaaatcc ggcgcgcgca taccgcgagt caccgacgcg gaaagcgaca gccgctgaaa
     5461 gggtttccac gccaacgctg cctgtggaga cagccgccca ccaaaatttg tgtcgtggtc
     5521 cgcgcgataa cccgccatca atcgcagtga gcggaccagc gggatctcgg cggttgtcac
     5581 ctgcgaccag acgtcggtct cggcacggtt gccttccgtg ttgatccggt cgccgaccat
     5641 gctttggcga ttgaagaaca aacccgttga aaggcgaaat ccgcgcgacc gcgcgagcga
     5701 ccactccaca tctccaccgt tggcttcatt gtgcgtcgac tgaggccgca gactgagagg
     5761 gtcccggtaa gccagattct catccgaccg gtagtagagc ttgcgtccgc gcacggtcaa
     5821 caggctgtcg ctccccagca gaagactggc tcgcggcagg aataccaggc gccgtttcgg
     5881 tctttcgaaa tcatagattc ccgtgacggt gctgccttcg aagccccaga agttctgatc
     5941 ggtccagagg aaggtcaggc cggtatttaa cctcttccac tgcggcagat acaaactgct
     6001 gaacaggttt cgcatccggt tggcgctttg tgtcttccca aggatgtccg attcgagatc
     6061 gtagcccagg ctacgctgcg ccgctccggc cgcatagatc gcgaagccgt ctttcgcata
     6121 gccgccgtct gcacggagca accccgtatt gaacgaaccg tagccggttt ccacggatgc
     6181 gtgcgatccc gtgccggctc gcttggtgat cagattgatc gcaccaccca tgccctgctg
     6241 tccgtacgtc atggcgcttg caccggtctt gatctccaca gactcgaaca tggaggcgtc
     6301 gaactggttg agatcaacat agccgctcac tcttcccgtg agcggttcgc cgtccaccag
     6361 aatctctaca tcccgcgaag caaagccttg cacgttggtg ctgccgccgc ccgacatggt
     6421 gcctccagcg aaggtgacga cctccggttg ctcctggagt gcatcggtca actggcgcgc
     6481 accgatctgc gtgatggcgc cggcatccac ctgacgcacc ggaagcggcg catccaggag
     6541 cgattccgga agacgcgatg ccgtcacctc aatttgactg gcgacagtcg agagccgcag
     6601 agcgagactc aaacgctgtt cgcccctggg ttccaccgta acgcttgccg ccgccccttc
     6661 catcccactc ccaaaggccc tgacgacata cacgcctgcg cccgtatcgg cacaccactc
     6721 accgtcttcg cccgtgacgc cgcgcaggac tgccgatgtc gccaatggtt caacgcgaat
     6781 ggcggcggcg ttgaccggaa gcccttgcgg gtctttgacg gcgatacaaa gacgctggct
     6841 ccatgcaggg gccggcaaca gcaggagccc caacacgatg atctgaatga aaaatgattc
     6901 cttacgcatg ttcttactcc tttccgccag tgcaaatcat ttgcatatca atccgcttaa
     6961 ggcaggacgt tggcccgcat tatccaatca tgcctttgcg cggagatctg atgcgtcaaa
     7021 cgcaataatg caattgactt gcattatatg actgtgactg tagtcacgca ataggcgatt
     7081 tcgcgttcca cactgctaca ttggtctggc acgacgagcg aaattcgcaa atgggttgcc
     7141 gatggcaagg agaatccggt cgaatgagca cgtcgccgag gggctggaac tactggggct
     7201 tgaggaattg gtcgggaggg gtttcgaggt gggtaaattt cttagcagta ctacagtgcg
     7261 gatgattcag aaggtttgtg ccgggagttc aacctttttc ctcaagccct cgaacttttt
     7321 tgcttacgct acgggtaatg caatcccaag accaatccgt aagtttaaga ttcaaaagga
     7381 aaccgcgagg aagggttctt tatcgctctg gaaaatttgc acgggcggaa cagggcgtcg
     7441 tgcaattctg aaacagcttg tctgctcagc gggggccggg ggcgagccgg attgtctggc
     7501 cgcgcccccg agcccgtttc atttgttctg ttgcggatcg attaccggga ttggagacgg
     7561 ccgggtggag acttccccgc gcaccgcatc ttcaatcagg acattgttga tttcgagcgt
     7621 tcttccggtt gcccgatcca tctccacccg cgaacgcgcg tattggctca tcgccgtcac
     7681 ctcgtttgcc tttgccaggg ccagatcgcg ctgcgactgg atgatttgaa agatggtgga
     7741 agcgcccagt gcatattttt tctgctcggc gtcaagcgtc tgctcctgca gcacccggtt
     7801 cttcaccgcg gtctggtagc gcgcccgggc ctgctggatt ccgatcagag cgttctgcac
     7861 ctccactcga atggcattca cctgctgctg ttggcgtagc tcattctgcc ggagggtcaa
     7921 ctggttagcg atcacgtccg cctgcgccga gcggttacgc aacggaatgg tcatctgcac
     7981 gccaaacgaa tagtccggga agttgcgccg gaacaactgg ccgacagcgg tgccaaaacc
     8041 gccgcggaag aacggatcga tggcgtccgg atcgcgaata accggcgctc ccccggtttg
     8101 ggagggaacc gggatcgtat tcacctgccc ggccagggca ttgttctgca attggatgaa
     8161 accatcgagg cgcggaagca gcgcgttctt ggaagccctc agcccgatgc gggagttctc
     8221 gagctgaaac cgcgtttcag acagttccgg acgcttttcc atcgcggagg ccaccaggtc
     8281 ctgtatcggc tcgatcgcct cgacatcggg aatccgaatg tggtccgtag gaacgatgcg
     8341 cgcctcggca agtagcggcc cagccacacc cacgcggctt agcgcgtcct tcagaatcgt
     8401 ctcctgctgc aggacgttgg tttcggctac ggtcagatcc tgttggcggg cagctacttc
     8461 cgcttccgcc cggatgattt cgatcggcgc aagagttcca atttccactt gtttccggtt
     8521 atccgacaac agcttctcgg ccaacttcaa cgcctgctgc cgcaccgcta catcttcgtt
     8581 gaatgcaacc aggtcccagt agaggttgat taccgaggcc acggtggcga tcaactggcg
     8641 ccggaacacc aggtccgaca cctgcacatt attcttcgcg atgtggatga accgggagtt
     8701 cagcgcaagt ccaaacccct ggagcagagg ctgcgtcagc gtgagattca gggccgacgt
     8761 tgtgtaaggg ttgaagtcga aacggcccga actggtggac tggtcttgat tggtcatgcc
     8821 aaggctgacg cgagtacccg tcaggaatcc ttgtgaaagc tcgaaattga agttcttgtt
     8881 ggtcgacacg acgttgttgg cgcccaccag gaacgagttc acctgcggtt gggtgcggtg
     8941 gccccaactg atggtgctgg taaaggaagg atccaggctt ggcactcctc cagtcgttcc
     9001 gcttccgccg attccgccac tgccgcccac acccccctgg gccagtgagg cgccagtcaa
     9061 atccgcgccg cgggtcccgc tgcccgtcgc actcgaagct ccctgcgaca ccgtcgtcga
     9121 gaccccgcgg atgctgccgc cggcttgcgc gcgccgcaaa tcggtctcgg ccaaccgtgg
     9181 accgtaccgg gcgacttcaa tgtccagact gttttcaatg gccagcgcga tggcgtcctg
     9241 tagcgataga taaatcctgc ccgcacggat cagcgcatcc agccggctgg agttcgccag
     9301 attgatcggc ggaatttctt ttccccggta gggatccgtg aggctggaat gccatgcgcc
     9361 tccctcgtcc aggacggaac cgttcgcccg cggttgctgg gcggcggtcc atggcgccat
     9421 cagcaaaaac gcacaggcga tactcaggca cttcttaacg cagttcatgg tatcgatacc
     9481 tcgatagact caagtggaac ctgtaaaact ctatgacgga atgacgagcc ggtctgttcc
     9541 ttgatacgta catcgtagca agcgcgagag agagaaaaat cttccatcgc ggtatattgg
     9601 ggtcttgtga tccccaccag cgaggttctc gaactcttta agcaaaccgg agcttacctg
     9661 aaagggcatt tccgcctgac cagcgggctt catagtcccg agtacatgca atgtgcgctg
     9721 gtgctccaat acccggaata cgcggaacgg cttggacgcg aactcgcgcg cacattgcgc
     9781 agccttgtgg gagaatccgc catagacgta gtggcctcgc cggcgatcgg ggggctcatc
     9841 atcggccacg aggttgcccg cgccctgggt gcacgccata tcttcgccga acgcgatgcc
     9901 agtgggcgca tgatcctgcg ccgtggactc tcggccgcct ccggtgagcg cgcggttgtc
     9961 gtcgaggacg ttatcacgac gggtggcagt tcgcgggagg tcatccagct cctgcgcgaa
    10021 tccggcgcca acgtggtggc ggccgcttcc atcgtcgacc gcagcggcgg ccgcgcaaac
    10081 ctggaggcgc cccacacggc gctcgctacg ctcggcgtca ccagctatcc gcccggtgag
    10141 tgcccgctgt gccggcaagg tctcccggtc gaaaagcccg gatcgagacc cgtttcatcc
    10201 caatgaggcg catccgcctt acactctcct acgatggaac ggggttttac gggtggcaga
    10261 agcagcccgg actcgccacc atccagggaa cgctcgaaca ggcggtgagt gaaatagaag
    10321 gcgcgccggt gcacgttgcc ggctcgggac gcacggatgc cggcgtacac gccgtggcac
    10381 aagtggcggc tatcagcatt gaaaacccca ttcccttacc gaacctgaag aaggcgctca
    10441 accgtctgct gccgtgcggc atacgcgtaa ctgccgtgga cgatgttccg cccgattttc
    10501 atccccgcta ccatgcgttg gcaaaaacgt acgaataccg cattgtacgc acggaggtct
    10561 gctcgccgtt cgaaagacat tacgcgtatc accatcctta tcccctggat acagcgcgca
    10621 tgttggaatt ggctccgatg ctcgaagggg aacacgattt cactgcattt gccgcttccg
    10681 atgagcgcga tgcgcttggg ctttccaaga tccgtacgat tttccgctcg gaactcgaag
    10741 aagtggacga ccggctgatt tatcgtgttc gcggcagcgg ctttctcaag cacatggtcc
    10801 gcaatattgt gggaacgctg ctggaagcgg gcaaaggcaa tctggacagt gagggactca
    10861 ggacacttct ccgctcgcgc gggcgaacgg gctgcgggcc aacggcaccc gcgcgaggat
    10921 tattcctgat atcggtcgaa tatcaaatgt aggcgatgac gtcgatctcg acaagcgagt
    10981 cgcccggaat gccgcccgct gcggcgatgg tggtgcgaac cggtggttca tcgccgaagc
    11041 ggcctcgaaa gacctcgttc atctctttgt agtctttgag atcgttcagg taaacgttgc
    11101 atttcaggac cttgttcatc gaggacccgg cattttcgag ctccttctgc acttcgtcga
    11161 gaacgtgctt cgtgtgtgcc ttgatgtcgc cctcgaaatg cgcgcctttg ccggagatga
    11221 acaggaggtt gccgtaagat accgcgccgg aaaacaaggg cgtgccttga ggctttttcc
    11281 cgcccctgta atgcaccttt ttctgcgggc cggactgggc ctttgcgaga cctgcgccag
    11341 ccgccatgcc gatcgctgcc ttcagaaccg ttctgcgttt gtctttcatg attttcctcg
    11401 tttttcaggt attagttgtg ccggcatctc ttacagcact ccgccgccga tggcaagcct
    11461 cagcagccct aacccggtgg cgatcgcaca acaaatggtg ccggcgatcg agccggtgcg
    11521 tgaattcgga ccggccgttg ccagagcgat gatgctcacg accaggccgg ccagggcaaa
    11581 tggaatgttc aaccaattca gggcgccgag gcacggaaac atccccacca acatcccgac
    11641 gagtgccaga atgccccaca ctaagctcaa cgtttgcata tcagatttct ccagaataac
    11701 tggacagggg tgagcggcgg cttcgaatcc gcctgcaaag aaataccagc agtgttgctt
    11761 cgttcgccgt gaaggccgtg tacagcggaa ggctcgcccg gttttcgcgg ctggcgtttt
    11821 gaaactgccc ccgcgacaga cagagaaaag cccgagtcat gccgcacagc gaacacccgg
    11881 tgccgtgaat tctcttcgct tcacacaccg gagaccagcc acgaatcgtt tcttcagaca
    11941 gcagaaaggg cgagacaagg attgcgagaa tcgccacgct caggacaatc cacgataaaa
    12001 caaacgctcg ctttaattcg gggcagcggc ccggcatccc cgttattgta tgcgagcaag
    12061 cgccgtcagt gaataagggt gcggctgatg gcctcgaaat gggtatggct tcccatctct
    12121 cgccctctca gcgattgcgg atgcagcagc ggttgacttg cccatagtgt tcctcagcga
    12181 gtggctttgc cagatcgtgt tggctggtgc ccgatgggta attcaatatc agatctccct
    12241 cgtgcaggat cgcaaccggc aaaaagtaag tgataggcct ctccaggttc caaatcgctg
    12301 aacgacttca agcgaatact ccgagcatgt aggtttgtac cggcaccgaa taaacttgct
    12361 cgtcaatggc cgcccgtatc gctgatacga tcgcacggaa gccatccaga gccttgcggt
    12421 catctgttta cttggcggac gcgcaacgtc tgctgccgcg agtccaatca acagaattag
    12481 ggcatacaag taaatatgtg gtgttcttac gcatttccgc agtgggggca tcgggcactc
    12541 gactgaaggc gcctattgtg gcaatgagag cattgaatta cgtttccctg gggccgcgac
    12601 aacagataga taacaaaccc aacgggacca gtaaacataa ctaacagcat ccagatgacg
    12661 gcactatcca tcccacgagc cttggcgtct ctcgcaaccc aaatcagcag cgcgatattc
    12721 aagacgatca acacaattgg gattaggatc aaagacccac atgctgcaca gccggcggcc
    12781 tcgcccgcgc cgttcgacct acgctgtcca atagcgcttt ccacgaatat caatgtcagg
    12841 ctcaaaaacc acaggtatct gaggcgcaca aacagcccat cctttccgat agcatctcaa
    12901 tctttcgtga tctgctcgcc ctaatatcgc caagtccaaa gacagaaatt agctaacgct
    12961 gtgcggcggc agcgcttggg ttcttgacgt agtcttgccc aaccatga
//
LOCUS       DASTMK010000182         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_265172, whole genome shotgun sequence.
ACCESSION   DASTMK010000182 DASTMK010000000
VERSION     DASTMK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265172"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VFQ79_07820"
     CDS             complement(<1..1535)
                     /locus_tag="VFQ79_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0139601.1"
                     /translation="MKKLASRLLVALIALAVLLTPVLAQTGTGTVRGTVYDPTQAGIP
                     GATVILMNQKTNDQRRTSTTSEGIYSFAAVPLGSYTVSIEASGFKKWEGTLELQVGQT
                     AVANATMQVGSVDTVVEVTGAAPVITTESAEVADVKDALRIQQLPLNGRDVSSLFTLT
                     PGVEGGGSPRVNGLKVGATEMLLDGISVVDRFGGGISRVQPGLDTVQEFRIETIGSSA
                     QYSRPATITLVTKSGTNEFHGTLFETHRNNAGGLRARQRQDGNTAAKLIRNEYGVSAG
                     GPVYIPQLFDGRDRTFWFAAWEGLKQRESLFARGSVPTEAMWRGDFSGLVDSAAQRTT
                     TYDPMTTNAEGRRQPFAGNLIPTSRQNPFFTKMRDVTPMPTNDVNPYIGENFQAVYPN
                     TNDVYKFTAKGDHRFSDKDTLTGRFSVSNLSQKLHGGRFSIPPPTMENAYGSGRTDAK
                     IYTPNIRYTRIFSPTLLNELNLGLHRSAKSSGTLADNTSWANDLGLPNPFGAQGWPTL
                     YTD"
     gene            1704..2981
                     /locus_tag="VFQ79_07825"
     CDS             1704..2981
                     /locus_tag="VFQ79_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU0139602.1"
                     /translation="MRKGGGLRGLALHHVRGANRATVLQLLDRQQQISRADLARQTGL
                     SEGTVSRIVGELIRERLVIERGVENSTGGRPGKRLELNQSGIHAIGVEIQSWETRISV
                     GTLGGRILEDRSFLTPENPLKTLDLVSEHIAAIRRRHAADMFEGVGVAVRGIVNSATG
                     VLELGSLPAWEGIAIREYLEASVAGPVVLENNVRAAALAEYRHGPADIQNSRSMLFIK
                     IDEGIGMGLIFDGHMYFGPHMAAGELGQMVIADSPGGGREDRPGCLESLASNPALCER
                     YAQLSGSPYLTKAGETTARARRICHLAMQGDAAARAALAENCRFLGIGIANAVWALDA
                     DAVIIDGVITEAWPLVVPAIREQFPAGRVFRNFHELMLRPSSLGSDGTIIGAVATAFS
                     PLFASGHRAGVKAAPGKRRHAVSSFVTPGTNTG"
     gene            complement(2936..>3822)
                     /locus_tag="VFQ79_07830"
     CDS             complement(2936..>3822)
                     /locus_tag="VFQ79_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139603.1"
                     /translation="AIDPAGSKAVRAACDLAKKKNLCVVSGLQSRYHPGYRETIQRIH
                     DGAIGDIIAVQEHFLRAPYVLYPRKPDLSEVEWQGSNQYHFHWLCGDDVPQSLIHNLD
                     RASWAMHNDAPLKCHGMGGRSTLRGEIYGSVFDHHAVVYEFASGVRIYAFCRTIPDCY
                     NENSSIILGSKGRCDVMRMTIEGETKWQYTGPRTYSGPTSNPYFIEHAELFKAIRSGR
                     AINNGDYMVRSTLIANMGQISCYTGKEVTWEQISTSDFYFAPKPEEVRPDMEAPVKPD
                     ADGNYPVFVPGVTKLLTA"
BASE COUNT          735 a         1110 c         1150 g          827 t
ORIGIN      
        1 cagtccgtat aaagtgtggg ccaaccttgc gcaccgaacg gattcggcag gcccagatcg
       61 ttcgcccagg atgtattgtc agccaaggta ccgctgcttt tggcggaccg gtggaggcca
      121 aggtttaact cattcaacaa agtcggagag aagatgcgcg tgtagcggat attcggcgta
      181 tagatctttg catcggtgcg gccgctacca taggcgtttt ccatcgtcgg gggtggaatg
      241 ctgaaccggc cgccgtgtaa tttctgggag aggttggaca cactgaacct tccggtcagc
      301 gtgtctttgt cggaaaaacg gtggtcaccc ttcgcggtga acttgtaaac gtcgttcgtg
      361 ttcggataga cggcctgaaa gttctcgccg atgtaggggt tcacatcgtt ggtgggcatc
      421 ggcgttacgt ctcgcatctt tgtgaagaac ggattctggc ggctcgtggg aatcaggttc
      481 ccggcaaacg gctgccgccg tccctcggca ttcgttgtca tggggtcgta agtagtcgta
      541 cgctgtgcgg cggaatccac caggccgctg aaatcgcccc gccacatggc ctctgtcggt
      601 acggagccgc gggcaaataa actttccctc tgcttcagtc cttcccaggc ggcaaaccag
      661 aatgtgcggt ccctcccatc gaagagctgc gggatgtaaa cgggtccgcc agccgaaacg
      721 ccgtattcgt tgcggatcag tttcgcggcc gtattgccgt cctgcctctg gcgggcgcga
      781 agtccgcctg cattattccg gtgcgtttca aagagcgttc cgtgaaattc gttggtcccg
      841 ctcttggtaa ctaaggtaat agtcgccggg cgtgaatact gtgccgaaga gccgatggtt
      901 tcaattcgaa attcctggac ggtatcgagg cccggctgca cgcgcgagat gccgccgcca
      961 aagcggtcca ccacggatat gccgtccagc agcatttccg tcgcgccgac tttcaagccg
     1021 ttgacacgcg gcgagccgcc gccctccaca ccgggtgtca gtgtaaacag gctgcttacg
     1081 tcgcgaccgt tcaaaggaag ttgctggatg cgaagcgcgt ccttcacgtc cgccacttcc
     1141 gcgctttccg tggtgatgac aggggccgcg ccggtcacct ccactacggt gtcaacgctt
     1201 cccacctgca tggtggcatt cgccaccgcg gtttggccca cctgcagctc gagcgtccct
     1261 tcccacttct tgaaacccga tgcttcgatc gacacggtgt agctcccgag tggaaccgcg
     1321 gcaaaagaat agatcccttc ggaggtcgtg ctcgtcctcc gttgatcatt ggtcttctga
     1381 ttcatcagaa taacggtggc gcctggaatg cccgcctggg tgggatcgta aacggtgccg
     1441 cgcaccgttc cagtaccggt ctgagccaac actggggtga gcaatacggc gagagcaatc
     1501 agcgcaacga gcaagcggct cgcaagcttt ttcataacta tatcccctga tcgatttcgg
     1561 ttaaagtatc tttacttgcc actcgcacga atggcatttc atctactcaa cgagttgctt
     1621 ggagtataga caagctaatt tcgggtgtca agtcattcga aaaggtattt actttccttt
     1681 cacaattaag taggctgttc atcatgcgaa aaggtggcgg gcttcgcggg cttgcgttgc
     1741 atcacgtgcg gggtgcgaac cgcgctaccg ttctacagtt gctcgaccgg cagcaacaga
     1801 tctcgcgggc cgacctcgca cggcaaaccg gactctccga aggcaccgtt tccagaatcg
     1861 tcggcgaact gatccgcgag cggcttgtca tcgagcgcgg cgttgaaaat tccaccggag
     1921 gccgtccagg caaacggctg gaactgaatc agtccgggat acacgcaatc ggggtagaga
     1981 ttcagagctg ggaaacccgc atctccgtgg ggactcttgg cggccgtatt ctcgaggatc
     2041 gctcgttcct gaccccggag aacccgctga agacgctgga tcttgtttcg gagcacattg
     2101 cagcgatccg gcggcgccat gcggcggaca tgttcgaagg ggttggagtg gcggttcgcg
     2161 gtattgtgaa cagcgcgacc ggagttctgg aactaggcag tttgccggcg tgggaaggaa
     2221 tcgcgattcg cgaatatctc gaagcgagcg tcgccggtcc ggtggtgctt gaaaacaacg
     2281 tacgcgccgc ggctctcgcg gaatatcggc atggcccggc ggatatccag aacagccgct
     2341 cgatgctatt cataaagatc gatgagggca tcggcatggg gctgatcttc gacggccaca
     2401 tgtacttcgg accgcacatg gcggccggcg agcttggcca gatggtgatc gccgattctc
     2461 cggggggcgg acgcgaagat cgtcccggct gtctcgaatc gctggcatcc aatcccgcgc
     2521 tgtgtgaacg ctacgcgcaa ctcagcggat cgccgtatct gacaaaggcc ggggaaacca
     2581 ccgcgcgcgc gcgccgcatc tgccacctcg ccatgcaggg agacgcggca gccagggccg
     2641 ccctcgccga gaactgccgt ttccttggca tcggcatcgc gaatgccgta tgggcactgg
     2701 atgcggacgc cgtaatcatc gacggcgtca tcacggaagc gtggccgctt gtcgtcccag
     2761 ccattcggga gcagtttccg gccgggcggg tcttccgcaa ctttcacgag ctgatgttgc
     2821 gtccttcatc gctcggctcg gatggaacca ttatcggggc agtggcgacg gccttttctc
     2881 cgctgttcgc gtcgggacac cgcgcaggcg tcaaagcggc gcccgggaag cggcgtcacg
     2941 ctgtgagcag ctttgtcaca cccggtacaa atacgggata atttccatcc gcgtcgggct
     3001 tcaccggagc ttccatgtcc ggccgcacct cttcaggttt cggagcgaag tagaaatccg
     3061 acgtggaaat ctgctcccag gtcacctctt tccccgtata acaactgatc tggcccatat
     3121 tcgcgatgag cgtgctgcga accatgtagt cgccgttgtt gatggccctt ccggagcgga
     3181 ttgctttaaa caattccgca tgctcgatga agtaaggatt ggaggtgggg cccgagtagg
     3241 tgcggggacc ggtgtattgc cacttcgttt cgccttcaat ggtcatccgc attacgtcgc
     3301 agcggccttt gctgcccagg atgatgctgg agttctcgtt atagcagtcc ggtatggtgc
     3361 ggcagaacgc gtaaatgcgg acgccgctcg cgaattcgta tacgacggca tgatgatcaa
     3421 atacgctccc gtagatctcg cctcgcagcg tagagcggcc tcccatgccg tgacacttca
     3481 gcggcgcgtc gttatgcatc gcccagcttg cgcgatccag attgtgaatc agcgattgcg
     3541 gcacatcgtc gccgcacagc cagtggaaat ggtattgatt gctgccctgc cactctactt
     3601 ccgaaagatc cggctttcgc gggtaaagga catagggcgc ccgcaggaag tgctcttgca
     3661 cggcgataat atccccgatg gctccgtcgt gaatgcgctg aatggtttcc cggtagccgg
     3721 gatgatagcg gctctgcaag ccggagacca cgcagaggtt tttcttcttc gccagatcgc
     3781 aggccgcgcg cactgccttc gatcccgccg ggtcgatcgc at
//
LOCUS       DASTMK010000183         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_105363, whole genome shotgun sequence.
ACCESSION   DASTMK010000183 DASTMK010000000
VERSION     DASTMK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105363"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..601
                     /locus_tag="VFQ79_07835"
     CDS             257..601
                     /locus_tag="VFQ79_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEU0139604.1"
                     /translation="MCPELLDAPGFCMAHAKKLRQADDQRRGSSASRGYGGQWRKRRA
                     AWLAAHPLCEDPFGAHGGRPVPATDVDHMIPKRFGGADDESNYQSLCHECHSRKTRTE
                     MNQSSERFRMSA"
     gene            634..1362
                     /locus_tag="VFQ79_07840"
     CDS             634..1362
                     /locus_tag="VFQ79_07840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU0139605.1"
                     /translation="MAKIVREGVNLVVCDLPYGITRCEWDRPINLKRLWEQYERVLAP
                     NGAVLLFAQSKFMAALANSSPHFRYDLVWDKSYCTGFQNARRMPLRRHESILVFYRSL
                     PAFHPQGLVPLSKDRSPTIKPSEVYGRLRGVAKGKSMTGFPQSILRFPRDHKAKPCEK
                     PIDLLEYLIRTYSNEGDVVMDNTMGLGGTGLAAVQSGRRFIGIEIDRERFAIAKARIT
                     HLMASGGGCGKRVEGEGRVESLAA"
     gene            1424..1867
                     /locus_tag="VFQ79_07845"
     CDS             1424..1867
                     /locus_tag="VFQ79_07845"
                     /inference="COORDINATES: protein motif:HMM:NF016975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEU0139606.1"
                     /translation="MAGRPKAAKAKNLEGNPGKRPVREPVRGELGIDRMPSHLAGQAA
                     VHWRFFVDHLQKRGDLEKIDGPALADCCICFARMLGAEKAIEELGILVEGARGLVKNP
                     ALQIAREYRTKAQRYIELFGFAPGPRNRMTMPDLNEDADPQGLLD"
     gene            1874..2830
                     /locus_tag="VFQ79_07850"
     CDS             1874..2830
                     /locus_tag="VFQ79_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEU0139607.1"
                     /translation="MPFDAKQAGDAVRFFECYLKHTKGRFAGTPFLLPPWQAKIIRDI
                     FGTLGPDGFRQYRTAYVEIPRKNGKSEIAAGIALKLLLADGEPGAEIYSAAADKNQAG
                     IVFRVAASMVRKSPALRKRCRIIDSSKTIVVPKTESIYRVLSADGPRQHGLNPSGIIF
                     DEVHTQTELGDGSLWEALTTGSDTRRQPLMFAITTAGIPGESPLWEQLNKEAKAVLHD
                     QTADPHFYAVLYAAGEEDDWTSEATWRKANPAIGDFLDIEKVRADCLKAQRNPVYENT
                     FRRLRLNQRTQQETRYIPIAEWDACTVPLLDLSYARNLATAA"
     gene            2764..3390
                     /locus_tag="VFQ79_07855"
     CDS             2764..3390
                     /locus_tag="VFQ79_07855"
                     /inference="COORDINATES: protein motif:HMM:NF042863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HEU0139608.1"
                     /translation="MGRLHCPITGPELRKKPCYGGLDLSSRIDLTAFSLVWPVEDRFL
                     CAEWYWMPSKAVRERTRKDGRPYEVWVRQGWIEAIEGPVVDERIVRRRIEQLGEIFDI
                     REIAYDKFRAQVLQMDLTDAGFQMIEFGQTTGWYDEPTKRLLELILQRRIVNEPNPVT
                     RFCADCVSVRKDVDERIKPVKPDRDKSGKRIDGIVTRIMALAVRLRSR"
     gene            3387..3581
                     /locus_tag="VFQ79_07860"
     CDS             3387..3581
                     /locus_tag="VFQ79_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139609.1"
                     /translation="MKVQSGTSGAMSSPVDRITFAAANAVGSQWGSAIGPITDTWFQV
                     SWTITGTTPSFTFICAVGAL"
     gene            3611..4021
                     /locus_tag="VFQ79_07865"
     CDS             3611..4021
                     /locus_tag="VFQ79_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139610.1"
                     /translation="MAVLVLKDARVEVNSVQLSDHVRSVTLTVNADEVEDTNMGDNTH
                     VRLAGLKDWSISVEFAQNFAAASVDATLFPLIGAAAFPVKVRPTSGVVGPTNPEYSGN
                     AILTEYSPIDGAVGDLATTSVNFVAAGDLTRATA"
     gene            4021..4389
                     /locus_tag="VFQ79_07870"
     CDS             4021..4389
                     /locus_tag="VFQ79_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139611.1"
                     /translation="MSRPTAPRFSIQLDRERHLCFDFYAQWKFEDQMGRPFFSTFLPL
                     AKALQAADITQISSKDLVALLWCGLVHEDPCLSMEDVAYMLHLGNIQEVMGSIVQAQV
                     AAAARNEDLPERPPEAQPAQ"
     gene            4401..4625
                     /locus_tag="VFQ79_07875"
     CDS             4401..4625
                     /locus_tag="VFQ79_07875"
                     /inference="COORDINATES: protein motif:HMM:NF021092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEU0139612.1"
                     /translation="MGLWSTGRYDLRLSDEEFWSMTPREFYALHRRYRDSIELLDTLV
                     ASISHHAHQLHRGRQPAKALKDFFLFQRKK"
     gene            4622..>5589
                     /locus_tag="VFQ79_07880"
     CDS             4622..>5589
                     /locus_tag="VFQ79_07880"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEU0139613.1"
                     /translation="MTLQELLVKLGLDASAFESGLNDSQSHLEKWADKIDDFGSAAMG
                     AGATLTAGFTLPLVGLGAAALSVAGDMEQNRIAFTQMLGSAEAAGQFLEELREFAAKT
                     PFEFPELVQAARNMKAMGFEAAAVIPMMTVIGDAAAAMGGGAAMVDRITTALGQMQAK
                     TKVAAQEMIQLTETGIPAWKMLADGIGVSIPQAMKMAEKGAIDAGTAINAILAGMQSE
                     FGGMMEQQSRTLQGQWSNLKDQLTAVLETIGAALIPFATTAIQAAAPILDVIKGMAEG
                     FGTLPGPVQTIIVAVAGLAAAIGPVLVAVGAMAAGISSIISLAATLG"
BASE COUNT         1179 a         1628 c         1753 g         1029 t
ORIGIN      
        1 ccgcacctat atcgaccgcg gcgacgagct gtggaagttc gcactgtacg acgacatcag
       61 ctacagcggc aaaatcttta gccagaacta tcgctggagc ggcgactact taaggtatcg
      121 cgggcagtga gcgcgacgaa tggcctgcta ccaggacgat tttccgggta ctaaaccatt
      181 acccgggtcg agaaaatcgc gtacaggtca aatctgcgca aatgcctcgc agccgaagca
      241 tccctgcaaa gggccgatgt gccccgagct cctggacgca cccggcttct gcatggcgca
      301 cgcgaagaag ctgcgccagg cggacgacca gcggcgcgga tcgtcggcgt ctcgagggta
      361 cggcggccag tggagaaagc gacgggcagc gtggctggcg gcacatccgc tctgtgaaga
      421 tccgttcggt gcgcacggtg gccgaccggt gccggcgacg gacgtggatc atatgatacc
      481 gaagcggttc ggcggcgccg acgacgagag caactaccag tcgctgtgcc atgagtgcca
      541 cagccggaag acacgcaccg agatgaatca atcaagcgag cggttccgga tgtcagcgtg
      601 atcaggcttt accgcgggga ctgcctgcag aaaatggcaa agatcgtgcg agagggggta
      661 aacctcgtcg tttgcgatct gccttacggt atcacccgct gcgagtggga tcggcctatc
      721 aatctgaagc gtttgtggga gcagtacgag agagtgcttg cgcctaacgg cgcggtgttg
      781 ctctttgcgc agtcgaagtt tatggctgca ctcgcgaact catcgccgca cttccgctac
      841 gacttggtct gggataagag ctactgcacc ggatttcaga acgcgcggcg gatgcctcta
      901 aggcggcacg agtctatcct cgtgttctat cgatcgcttc cagcgttcca cccgcaagga
      961 ttggttcctt tgagcaaaga tcggtcgccc acaataaaac cctccgaagt gtacgggcga
     1021 ttgaggggtg tcgcaaaggg caagtcgatg actggtttcc cgcagagtat tcttcggttc
     1081 ccacgcgatc ataaggcaaa accgtgcgaa aagccgattg acttgctcga atacctcatc
     1141 cgcacgtatt cgaatgaagg cgatgtcgtg atggacaaca cgatgggcct tggcggaacc
     1201 ggactggcgg cagtacaaag cggccgtagg ttcatcggca tcgagatcga tcgcgagcgg
     1261 ttcgcgattg cgaaggcgcg catcacccac ctgatggcga gtggcggcgg ttgcggcaag
     1321 cgggtcgaag gggaggggag ggtcgaatcc ctggccgcct agcgcggaga ccgcgctccg
     1381 cggaacgcga aaacgcccgc agtttgaaac cgatttttta cacatggcag gcagaccgaa
     1441 agccgcgaag gcaaagaatc tcgaaggcaa ccccggcaag cgccctgtcc gggaaccggt
     1501 ccggggcgag ttgggcattg accgtatgcc ctctcatctg gccggccagg cagcggtgca
     1561 ctggcggttc ttcgtggacc atctccagaa acgtggcgac ctcgagaaaa tcgacggacc
     1621 ggccctggct gactgctgca tctgctttgc gcggatgctc ggtgccgaaa aggcgatcga
     1681 agagctcgga atcctggtgg aaggcgcccg cggactggtg aagaacccag ccttgcagat
     1741 cgcacgggag taccgaacca aggcccaacg ttatatcgag ctattcggat ttgcgccggg
     1801 cccgcggaac aggatgacaa tgccagacct gaatgaggac gcggatccgc aggggctgct
     1861 ggactgatcg gatgtgccgt ttgatgcgaa gcaagctggc gatgccgttc ggttcttcga
     1921 gtgttacctc aaacacacca agggacggtt tgccggcact ccgttcctgc taccgccctg
     1981 gcaagcgaag atcatccggg acattttcgg gacgctcggc ccggacggat tccgccaata
     2041 ccgcactgcc tacgtcgaaa tcccgcgcaa gaacggcaag tccgaaatcg ctgccggcat
     2101 cgctctcaag ctgctactgg ccgacggtga gcccggcgcc gagatctact ctgccgcggc
     2161 agacaagaac caggcgggca tcgtgtttcg cgtcgccgcg tccatggtgc ggaaatcacc
     2221 ggcgctgcgc aagcggtgcc gcatcatcga cagctcgaag acgatcgttg taccgaagac
     2281 ggagagcatc taccgtgtgc tgtcggccga cggtccacgc cagcacggcc tcaatccatc
     2341 cggaatcatc ttcgacgagg tccacaccca gacggagctg ggcgacggca gcttgtggga
     2401 agctctcacg acgggtagcg acacccggcg gcagccgctc atgttcgcta tcacgacggc
     2461 gggtatcccg ggcgaatctc cgctgtggga gcaactcaac aaagaggcca aagcggttct
     2521 gcatgatcag acggccgatc cgcacttcta cgcagtgctc tatgccgccg gcgaagaaga
     2581 tgactggacc agtgaggcaa cctggcgcaa ggcgaacccc gcgattggcg acttcctgga
     2641 catcgagaaa gtccgggccg actgcctcaa ggcacagcgc aatccggtct atgagaatac
     2701 gttccggcgc ttgcggctca accagcgtac ccagcaggag acgcgctata tcccaattgc
     2761 ggagtgggac gcttgcactg tcccattact ggacctgagc tacgcaagaa accttgctac
     2821 ggcggcttag atctgtcttc ccggatcgat ctcactgcgt tctcgctggt gtggcccgtc
     2881 gaggaccgct tcctttgcgc tgagtggtac tggatgccct cgaaggcggt ccgggagcga
     2941 accaggaaag atggcaggcc ctatgaggtc tgggtccggc agggctggat cgaagctatc
     3001 gaaggaccgg tggtcgatga gcgcatcgtt cgccggcgga ttgagcagtt gggtgagatt
     3061 ttcgatatcc gggaaatcgc ttacgacaag ttccgggcac aagtgctgca aatggatctg
     3121 accgatgccg gttttcagat gatcgaattc ggccagacaa cgggttggta tgacgagcca
     3181 accaagcgac tgctcgagtt gattctccag cggcggatcg tgaatgagcc caatccggtg
     3241 acacggtttt gcgccgattg cgtgagcgtc cgcaaagacg tggacgaacg gatcaagccc
     3301 gtgaagccgg accgcgataa gagcggcaaa cgcatagacg gaattgtgac cagaatcatg
     3361 gcgctggcgg tacgccttcg ctcacggtga aggtgcaatc cggcaccagc ggcgcaatgt
     3421 cgtcgccggt cgaccggatc acgttcgcgg cggcgaatgc ggttggatct cagtggggct
     3481 ccgctattgg ccccatcacc gatacctggt ttcaggtgtc ctggaccatc accggaacca
     3541 ctcccagctt cacgttcatt tgcgccgtcg gcgctctgta aaacccctca acaaaagaaa
     3601 aggaaatcct atggctgtct tggtattgaa agatgcccga gtggaagtga actcggtgca
     3661 gttgagcgat cacgtgcgca gcgtcacgct gacggtgaac gctgatgaag tcgaggatac
     3721 gaacatggga gacaacaccc atgttcgttt ggccgggctg aaggactggt cgatctcggt
     3781 ggagttcgcg cagaacttcg cggccgcgtc cgttgatgcg acgctgtttc cactcatcgg
     3841 cgccgccgcg ttcccagtga aggtgcggcc cacttccggc gtcgtcggcc caacgaaccc
     3901 ggagtacagc ggtaatgcga tcctcaccga gtattcgccc atcgacggcg cggtgggcga
     3961 tctcgcgacg acgagcgtca atttcgtggc ggccggcgat ctgacgaggg cgacggcgta
     4021 atgagcagac ccacggctcc gcgcttttcg attcagctcg atcgggaacg gcacctctgc
     4081 ttcgatttct acgctcagtg gaagttcgaa gaccagatgg gaagaccttt cttctcgacg
     4141 tttctgcctt tggcaaaggc tctgcaagcc gccgacatta cgcagatctc cagtaaggac
     4201 ctcgtcgcgc tcctgtggtg cgggctggtg catgaagatc cctgcctatc catggaggac
     4261 gtggcctaca tgcttcatct aggcaatatc caggaggtga tggggtcgat cgtccaggca
     4321 caggtcgccg cggcggcccg caacgaggat ctgcccgaac gccctcccga ggctcaaccg
     4381 gcgcagtaag cactgactgg ttgggcctat ggtccaccgg ccgctacgat ctgcggctgt
     4441 cggacgaaga gttctggagc atgacgccac gcgagttcta cgcccttcac cggcgttatc
     4501 gcgattcgat cgagttgctg gacacactgg ttgcctcgat cagtcaccac gcccaccaac
     4561 tgcaccgcgg gagacaaccc gcgaaagcac tcaaagactt tttccttttc cagcgtaaga
     4621 aatgaccctt caggaattgc tcgtaaagct ggggctcgat gccagcgcgt tcgagagcgg
     4681 actgaacgac tcccagtccc atctcgaaaa gtgggcagac aaaatcgatg acttcggcag
     4741 cgccgcgatg ggggcggggg caacgcttac tgccggattc acgctaccac ttgtggggct
     4801 cggcgcagca gctctttccg tggccggcga catggagcag aaccgcatcg cgttcacgca
     4861 gatgctgggc tcagcggaag cggcaggcca gttcctcgag gaactgcgcg agttcgcggc
     4921 aaaaactcct ttcgaattcc ccgagctggt gcaagcagca aggaacatga aagcgatggg
     4981 attcgaagcc gctgctgtca ttcccatgat gacggtcatc ggtgacgctg ccgcggcgat
     5041 gggtggtggc gccgcgatgg tggaccgcat cacgaccgcg ctcgggcaga tgcaggcaaa
     5101 gaccaaggtg gctgcgcaag agatgattca gctcaccgag accggcattc ctgcgtggaa
     5161 aatgttggcc gatgggatcg gtgtgagcat ccctcaagcc atgaagatgg ccgagaaagg
     5221 ggccatcgat gccggcacgg ccatcaatgc cattctggcc ggcatgcagt cggagttcgg
     5281 gggcatgatg gagcagcagt cccgaacgct ccaagggcaa tggtcgaacc tcaaggacca
     5341 gttgactgcc gtcctcgaaa caatcggtgc agcactgatt cctttcgcaa ccactgcgat
     5401 tcaggcggcg gcgccgatcc tggatgtaat caaggggatg gccgaaggat tcggcaccct
     5461 gcctgggccc gtgcagacga tcatcgtcgc agtcgcaggc ctcgccgcgg ccattggtcc
     5521 agttctggta gccgtcggcg cgatggctgc cggcatatca agcatcatca gcctcgccgc
     5581 gacgctggg
//
LOCUS       DASTMK010000184         4392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_10244, whole genome shotgun sequence.
ACCESSION   DASTMK010000184 DASTMK010000000
VERSION     DASTMK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10244"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VFQ79_07885"
     CDS             <1..1327
                     /locus_tag="VFQ79_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HEU0139614.1"
                     /translation="THKGPILTVANWSGEWPGLVGMLNLNGCLTKAGVRYSTLWSKDF
                     DDEFFLNNLRKWLAGEQIVHDTSHVRKLSDFKLPARAEETGRELAAGLVRNKAILGIF
                     DEGCMGMYNAIIPDELLHQTGVFKERLSQSALYARMREVPDDEAHPVRAWLDGKGMKF
                     RTGPNPETDLTDDQILKQCKMYIAALRIAGDFGCDAIGIQYQQGLKDLAPASDLVEGL
                     LNNVDRPPVKAEGNGRVLYEGQALPHFNEVDECAGLDALVTNRIWNKLGFDPENTLHD
                     VRWGEAYAVNGKEEFVWVFEISGAVPPKHLTGEYAGAVSERQPPMYFRLGGGTIKGVS
                     KPGEIVWSRVFVDGGNLKADLGRARAVELPQEETERRWRSTTPQWPIMHAVTYGVTRD
                     QMMARHKANHIQVAYAPDVEGANLALAAKAAMFRELGLEVSICGDRHGL"
     gene            1377..2402
                     /locus_tag="VFQ79_07890"
     CDS             1377..2402
                     /locus_tag="VFQ79_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139615.1"
                     /translation="MRVGIIGTGAISHKHAQAYKNIGYTVTVCTDVYEPAGRKFAEQY
                     GCTFVPTYEELCRHPEVDFVDVCTFPDFRLQPLEICAEAKKHIQVQKPISTNLETARK
                     MIDIAKNAGIQLGVVSQHRFDDSSQFLSKAIEAGRLGKILQADAYVKWYRSAEYYSRP
                     IKGSWETEGGGAMINQAIHQVDVLLWLVGPVKELFAYWQLGALHKIESEDVVNALMRY
                     ENGATGVIQASTAFWPGYTERLEIHGTKGTAVISGDKLTTWDVQDDSGDPAPVEKEVM
                     SGSSDPMAISLTPFERQFRSFGEAIMTGTKPFISGEDGYRALELVLSVYKSSREGQKV
                     TLGGKAN"
     gene            2409..3323
                     /gene="thrB"
                     /locus_tag="VFQ79_07895"
     CDS             2409..3323
                     /gene="thrB"
                     /locus_tag="VFQ79_07895"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU0139616.1"
                     /translation="MSSWLTVRVPGSSANLGPGFDALGLALRIYLTCRFRRSETLIIR
                     TCGRDASMISTGEDNLIWQTVLAVARDTGMTATPLELEIDNDIPIGKGLGSSAAALTA
                     GVVIASRLFDLGWKPLRILDEAAQIEGHPDNVAACVLGSIVASAIDSGGVARAVRIEL
                     PERYGVAVVVPDFILPTSEARSVLPNSYSRADTIFNVQRSALLIAALATGTTSAFPAA
                     LEDRLHHPYRAPLVPGLEDILKLRAPGLLGCALSGAGPSVLVFYERNCAQVCDLVSQI
                     FKLHGHSSEILWAQIADHGYELVESPAS"
     gene            3320..4162
                     /locus_tag="VFQ79_07900"
     CDS             3320..4162
                     /locus_tag="VFQ79_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0139617.1"
                     /translation="MNKKRRHFTILLVAFALLPAAWAEQLKVMSFNVRLPSKADGENH
                     WDHRRDLLVRTIQKYDPDVFGTQELFHEQGEYIAAKAPEYKWFGVSRRGNQTDEHMGV
                     FYKPSKLTLLDSGNFWLSETPEVPGSMSWNVSLPRMVTWGLFQTTAGKKFYLYNTHFP
                     HRREDAEARTNCARVIAKRIGSLPTGTPLIVTGDFNTAAESSEPYTILTGESKDAWTT
                     APDRSGPTGTFNGFKGRDTGPRIDWILYRGDVQPVSAETITYNESGRYPSDHFPVMAV
                     FELK"
     gene            complement(4165..>4392)
                     /locus_tag="VFQ79_07905"
     CDS             complement(4165..>4392)
                     /locus_tag="VFQ79_07905"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEU0139618.1"
                     /translation="TGPNFYLLRGLVWLFIGIGFAIFLVVMSFADPDPETRAAAALGL
                     IPAGVGVAYLIVYRAENKKSRNTEIANSRVL"
BASE COUNT          939 a         1273 c         1344 g          836 t
ORIGIN      
        1 cacgcacaag gggcctattc taacggtggc gaactggagc ggcgagtggc cgggtctggt
       61 gggcatgctc aacctgaacg gctgcctcac caaggcggga gtccggtaca gcacgctttg
      121 gagtaaggat ttcgacgacg agttcttcct gaataacctc cgcaagtggc tggcgggtga
      181 acagatcgtg cacgacacca gccatgtccg gaaattgagc gatttcaaac tgcccgcgcg
      241 cgcggaagag accggccgcg agttggccgc cggcctggtt cggaacaaag ccatcctggg
      301 cattttcgac gaaggatgca tgggcatgta taacgccatc atccccgacg agcttctgca
      361 ccagaccggc gttttcaaag agcggctcag ccagtccgcc ctgtacgcga gaatgcggga
      421 agtgccggac gacgaggctc atccggttcg cgcgtggctc gatggcaaag ggatgaagtt
      481 ccgcacggga cccaatcctg aaacggatct cacggacgat cagattctga aacagtgcaa
      541 gatgtatatc gccgcgcttc gcatcgccgg cgatttcggc tgcgatgcca tcggcattca
      601 gtaccagcaa ggactgaagg acctcgcccc ggcatcggac ctggtggaag ggctgctgaa
      661 caacgtggac cggcccccgg tgaaagcgga aggcaacggg cgagttctct atgaaggcca
      721 ggctctgccg cacttcaacg aggtggatga gtgcgccggc ctcgacgcgc ttgtaacaaa
      781 ccgcatctgg aacaaactgg gttttgaccc ggagaacaca ctgcacgacg tccgttgggg
      841 ggaagcgtac gcggtgaacg gcaaagagga attcgtgtgg gtgttcgaaa tctccggcgc
      901 cgttccgccg aagcacctca ccggtgaata tgcgggcgcc gtgagcgagc gccagccgcc
      961 tatgtatttc cggctgggcg gcggcaccat caaaggcgtc agcaagcccg gtgaaattgt
     1021 ctggagccgt gttttcgtcg atggcggaaa tttgaaagcc gacctcgggc gagcgcgcgc
     1081 agtggagttg ccccaggaag aaaccgaacg ccgctggcgg agcaccacgc cacagtggcc
     1141 catcatgcac gccgtcacct atggcgtaac tcgcgaccag atgatggcac ggcacaaggc
     1201 gaatcacatc caggtggcat atgctcccga tgtggaaggc gcgaacctcg cgctggcagc
     1261 gaaagctgcc atgttccgcg agcttggatt ggaagtcagc atttgcggcg accgtcacgg
     1321 gctgtagccc tgtggcgctt gttatactgc cctattcacc caaggagaat tgaacgttga
     1381 gagttggaat catcggcaca ggagccattt cgcataagca tgcgcaggca tacaagaata
     1441 ttggctatac ggtaacggtc tgtacggacg tgtacgagcc cgccggacgc aagttcgccg
     1501 agcagtacgg ctgtaccttc gttcccactt atgaggagct ttgccgtcac cccgaagtgg
     1561 atttcgtaga cgtttgcacg tttcccgatt tccggcttca accgctggag atctgcgcgg
     1621 aggcgaagaa gcacatccag gtgcagaagc cgatctcgac caacctggaa actgcccgca
     1681 agatgatcga catcgcgaaa aacgccggca tacagttggg cgttgtcagc caacatcgct
     1741 tcgatgattc gtcacaattt ttgtcgaagg ctatcgaagc cggacgcctc ggaaagatcc
     1801 tgcaagcaga cgcctacgtg aaatggtacc gctccgccga gtactattcg cggcccatca
     1861 agggcagttg ggaaaccgag ggcggcggcg caatgatcaa tcaggccatt caccaggtgg
     1921 acgtgctgct gtggctggtc ggtcccgtga aggaactgtt cgcctactgg caactcggag
     1981 cgctgcacaa gatcgaatcg gaagatgtcg tcaacgccct gatgcggtat gagaacggcg
     2041 ccaccggtgt gattcaggcc agtacggcgt tctggcccgg gtacaccgag cggctggaga
     2101 ttcatggcac caaaggaacg gcggtcatct cgggcgacaa gctcacaacc tgggatgtac
     2161 aagatgattc gggcgatccc gcgccggtgg agaaggaagt gatgtccggc tcttcggacc
     2221 cgatggccat ctcgctgact ccgttcgagc ggcagtttcg cagcttcggg gaagcgatca
     2281 tgaccggaac caagcccttc atttcgggcg aggacggcta ccgcgcactg gagctggttc
     2341 tgtccgtgta caagtccagc cgcgaggggc aaaaggtaac gttgggcggc aaggcgaact
     2401 gattcccgat gtcgtcctgg ctcactgtcc gcgtcccggg ttccagcgct aacctgggac
     2461 cgggcttcga tgcactcggc ctcgcgctgc gcatctatct gacctgccgc ttcaggcgaa
     2521 gcgagactct catcatccgt acgtgcggcc gggacgcgtc gatgatatcg accggcgagg
     2581 acaacctgat ctggcagacg gtgctggcgg tggcacgcga caccgggatg acagccacgc
     2641 cgctcgaatt ggagatcgac aacgatattc ccatcggtaa agggctgggg tcgagtgccg
     2701 cggcgctcac cgccggggtc gtcattgcca gccgtctttt cgatctcggt tggaagccgc
     2761 tccgcattct cgatgaagcc gcgcaaatcg aggggcatcc cgataatgtc gcggcgtgcg
     2821 ttctcggctc gatcgtcgcc agcgcaatcg actcaggcgg tgtcgcccgt gccgtccgca
     2881 ttgagttgcc ggaacgctac ggtgtcgcgg tcgtcgtgcc ggacttcata ctgccgacct
     2941 cggaagcgcg ctccgtgctc cccaattcgt actcgcgagc cgacaccatt ttcaatgtgc
     3001 aacggtcggc cctattgatt gccgcgcttg ccacgggaac caccagcgcg ttcccggcgg
     3061 cgctggaaga ccgtctgcat cacccctacc gggctccgct ggttcccggc ctggaagata
     3121 tattgaaact gagagcaccc ggtctgctgg ggtgtgccct aagcggtgcg ggcccgtcgg
     3181 tgcttgtttt ttatgaacgg aactgcgcac aagtctgcga tctcgtgagc cagatcttca
     3241 agctgcatgg gcacagttcg gagattctgt gggctcaaat cgcggatcac ggctatgaac
     3301 tggtagagag tccggcatca tgaacaaaaa gcgacggcac ttcacgatcc tgctggtggc
     3361 ctttgcgctc cttcccgcgg cgtgggccga acagctcaag gtgatgagct tcaatgtccg
     3421 gcttccctcg aaagccgacg gcgagaatca ttgggaccat cgccgcgacc tgctggtcag
     3481 gaccattcaa aagtacgatc ccgatgtttt cggcacacag gaactgtttc acgagcaagg
     3541 cgaatatatt gcagcgaagg ctcccgaata caaatggttc ggcgtaagca ggcgcggcaa
     3601 tcagaccgat gagcacatgg gcgttttcta taaaccttcc aagctcacgc tgctcgattc
     3661 cggcaatttc tggctgtctg aaactcccga ggttcccgga agcatgtcgt ggaacgtgag
     3721 ccttccgaga atggtgacct ggggtctgtt ccagacgacg gcgggtaaga agttctatct
     3781 gtacaataca cattttccgc accggcgcga agacgccgaa gcgcgaacga actgtgcccg
     3841 cgtgatcgcg aagcgtatcg ggagcttgcc caccggtact ccgctgatcg tcaccggcga
     3901 cttcaatacg gcggcggaat cgagcgaacc ttacacgatt ctgacgggcg agtcgaaaga
     3961 cgcctggaca acagcacccg atagaagcgg ccccaccggc acgtttaatg gcttcaaggg
     4021 acgagacacc ggtcctcgca ttgactggat tctataccgc ggcgatgtgc agccggtctc
     4081 ggcggagacc attacctaca acgaaagcgg acgctatccg tccgatcact tccccgtgat
     4141 ggcagtattc gagctgaagt aaactcacaa tacccgggaa ttcgcgatct ccgtgttgcg
     4201 ggattttttg ttctccgcac ggtagacgat gagataggca acgcctaccc cggcggggat
     4261 gagaccgagt gcggcggcgg cgcgcgtctc cggatcgggg tcggcaaagc tcattacgac
     4321 gagaaatatt gcgaatccga taccgatgaa cagccacacc agaccccgca acaggtagaa
     4381 gttcggtcct gt
//
LOCUS       DASTMK010000185        12465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_105396, whole genome shotgun sequence.
ACCESSION   DASTMK010000185 DASTMK010000000
VERSION     DASTMK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12465
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105396"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2939
                     /locus_tag="VFQ79_07910"
     CDS             <1..2939
                     /locus_tag="VFQ79_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139619.1"
                     /translation="ARKRQDGSAKPPQLIRNEFGGSAGGPVLLPKIYDGRDRTFWFFT
                     YEGNRTINPSTKGYQVPTEAMRNGDFTGLVDASGRQYAIYDPYTTNPATGEKQQISYQ
                     GRLNVIDPARMSPLAKYLYSITPLPTDPSVNPLIDNNWWGPAINTTRNWTTTARVDHR
                     FSDYDQFYARYTQGDLFRINDFYGQPMLDNVAGTNRTLAPNKSLATSYLHTFSPTLFN
                     ELMVSVSRENAFSGTGEPGVYYNDMLGLPNPMGVPGWPGIYDTGLSSYYFETQNTNRA
                     AFTNYILDNNATKIFGRHEFQFGGHLRYDQLNYLPEQQHPQGNHNFNSRATALVDPAG
                     ASTNPQPLPYTGSNLANMYLGLMNYSNQFVRGYFYARAKEYALYFQDNFRVNQRLTLN
                     IGLRWEKWPAYYEKNNMMTSFDPASNRIVLGQDLDTMYRLGGSVPSIVNRLETLGAKF
                     ATYKEAGMPKHLMSSGWRDFGPRLGFAYRAGDGTGSFVLRGGYRVSYFPIPLAPWGQR
                     MRQNAPLTARFVTSQTDAAQSPDGLPNWGMRSVPTVVAGVNSKDAVQLTNASLITRGQ
                     AVVSYFSPDQPDSKVHDWNFTIEKEVAKNTVARVAYLGNHVSNLEQYFRFNEATPEYI
                     WYATTGTRLPTGEFASVARRPFNQEVYGTMEEYRKSGWSNFQGAQFELERRYSQGFAY
                     QIFYNFGNAFTAGGKSYSGVVPNTNVFLPGAVPQDYDERNRLFNYQRDVTIPKHRLRW
                     NWVADLPFGKGKKFGGNAGGVLDKVIGGWQITGMGSLWSSYWQMPTTVYPNGNNVEVY
                     GYDYPIQDCRSGACRPGYLYWNGYINPNQINSYDANGKPNGVMGVPDNYKPAGQPLIP
                     WPLNPSTSDPMYAYYGSNTVWVPLNDGTTQRVNFDDGLHPWRQQYLPGPRQWNVDASI
                     VKNIRLTESVNLRFNADIFNVFNHPNNPAWTGSNTMPVAADGVYSTFTSGLESRELQL
                     SLRLSW"
     gene            3023..3856
                     /locus_tag="VFQ79_07915"
     CDS             3023..3856
                     /locus_tag="VFQ79_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139620.1"
                     /translation="MRKIICFWTLLCLPVAAQQKPLELVEAVFAQYEDGPPVPSGHKY
                     RAGETVFISFKVANYRVNQNNRIHLKYTVEALDPAGLKLVPPQSDQVSEELSPQDKNW
                     MPKIRFDALVPPTAAPGEYKIAATVTDEMGSGQKAAKEFPFQVDGSNMQPSDTITVRD
                     FAFYRTSEFTEPLQIAAFGPSDEVWARFLMTGYKFGPDNRVMVEYGVELLNPEGKVVF
                     SQSKAADFNEESFYPRRYVPGTASFRLTKVQPGEYTVRLTIRDLVGGQSNESTHPFRV
                     E"
     gene            3862..4509
                     /locus_tag="VFQ79_07920"
     CDS             3862..4509
                     /locus_tag="VFQ79_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEU0139621.1"
                     /translation="MESLSPVEVRVLGALIEKESTTPEYYPLSLNALVNACNQKSNRE
                     PAVNYDEDTVLAAIEALKARRFAAEIIPSSGSRVHKFAQRVSERLNLGRRELAVLCVL
                     LLRGPQTLGELRDRTERLHSFGDLDEVASVLERLAEWDPPLAVKLPRQPGTKEPRYAQ
                     LLGGTPDVQPASAEAPVAAQPRDERLASLEAEIAELRKEIEQLKQQFSDFRRQFE"
     gene            complement(4572..5909)
                     /locus_tag="VFQ79_07925"
     CDS             complement(4572..5909)
                     /locus_tag="VFQ79_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEU0139622.1"
                     /translation="MKVVLISTYELGRQPFGLASPAAWLKERGHDVMCVDLAITTLPS
                     LPIREAQLVALYLPMHTATRLAAQVIPKVRLLNPKVHLCAYGLYAPVNAGYLKKLGVE
                     TILGGEFEQALADLAERLVQDEYPVQQQLISLDRLEFSVPDRSSLPPLARYAKLHVNG
                     ATRIAGYTEASRGCKHLCRHCPVVPVYNGRFRVVQHDTVMADIRRQVEAGAQHITFGD
                     PDFLNGPAHARRIVEELCREFPGVSYDATIKIEHLIQAPDLLPVLRDTGCLFVTTAVE
                     SVDDRVLAKLEKGHTRADFIRAAGLLDAAGLVMAPTFIPFTPWTTRSGYSDLLRVIAE
                     LGLVENVSPVQLALRLLVTTGSRLLELPDIQERIERFDEAALIYRWRHEDPDIDQLAA
                     RVLSLVHDEQRRHAPRREIFRNIWQIVTDDPLPENFDLMPRATIPYMDEPWYC"
     gene            complement(5950..6753)
                     /locus_tag="VFQ79_07930"
     CDS             complement(5950..6753)
                     /locus_tag="VFQ79_07930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0139623.1"
                     /translation="MQAETTRNEGPWVLVTGASSGIGAELAKIFAAQGYSVALAARSV
                     SKLEELAAELANRYKARSRVIQSDLGDPDAPPRVYEELGKLGIALEALVNNAGFGLRG
                     FFAESDTGRLLEMMQVNMTALTHLTRLFLPGMIARRSGRILNVASTAGFVPGPTMAVY
                     YASKAYVVSLTEALANELSGTGVSITALCPGPTHTGFAETAGTSNARLFKRSSVMDAA
                     SVARIGFEGLMAGKTIVIPGLQNRIVMAAARIAPRKMLAGITRGLNSPE"
     gene            6776..8716
                     /gene="thrS"
                     /locus_tag="VFQ79_07935"
     CDS             6776..8716
                     /gene="thrS"
                     /locus_tag="VFQ79_07935"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU0139624.1"
                     /translation="MSNISITLPDGSRQEVQAGTTPLDLAKSNSRRLADDAVVARVNG
                     QLYDLTRPLESDASVQILTSRDPEALEVYRHSTAHLLAAAVLELYPGTKLGIGPPIDN
                     GFYYDFERETPFTPEDLERIEKKMWELQASDLPYERKLTPKEEGLKKYAAMGEPMKCE
                     LIEERADGIFSEYTLGPHFIDFCRGPHVPSTSRIKAFKLLSIAGAYWKGNEKNQQLQR
                     IYGTAFFSKKELEEYLKQLEEAKKRDHRKLGQELDLFSVQELAGPGLIFFHPKGGIVR
                     KLLEDWMRDQYLARGYSLVYTPHIARSDLWKTSGHYNFYAENMFKRMELDDAEYQLKP
                     MNCPFHILIYRDRMRSYRDLPVRLGELGTVYRYERSGVMHGLLRVRGFTQDDAHIFCT
                     PQQIEDEVVNCLQFALDVLSTFGFDKYEVELSTWDGGASGKYDGTPDQWQLGENALRK
                     ATERLDMQVKLSPDEAAFYGPKIDVKLVDAIGRLWQLSTVQFDFTLPRRFGLEYIAED
                     GKAHQPLMCHRALYGSVERFFGILLEHYAGAFPVWLAPVQAAVLPITDRQLEYARQVT
                     EQLRAAGIRPHLDDRNEKVNFKIREAQLQKVPYMLVVGDREAENGTVSVRNRKHGDQG
                     AKTLDAFLAEVKKLIDTKAPSE"
     gene            8721..9857
                     /gene="hpnI"
                     /locus_tag="VFQ79_07940"
     CDS             8721..9857
                     /gene="hpnI"
                     /locus_tag="VFQ79_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEU0139625.1"
                     /translation="MAVALALLLAAVAAGSLVYCILTVIAVSRYLAVAPPPLSSAEPV
                     SILKPLSGADEGLEENLRSFFRQKYPHFEILLAVRSESDAAVKLVRGLQAEYPEIPAR
                     LLITGEPPYPNAKVFSLDRMMAEARYDLLVMSDSDIRVTPELLQTIAGEFQDSNLGVA
                     TCPYRAVPGSSIWSRLEAIGMNTEFLGGVLVARMIEGMKFAVGPTIVARKRVIEKIGG
                     FARLREYLAEDFVMGKFAADAGYGVSLSSYVIEHRIGTEGLRANFAHRLRWARSTRRS
                     RPAGYVGQIFTNPLPVAVILVALEPAWWPALPVLAAARAAAAWAVSQRVLKDPLTRAH
                     WWLVPVQDFLSFVFWVAGFFGNSIQWRGRRYYLYRNGKFALLSK"
     gene            complement(9815..10342)
                     /locus_tag="VFQ79_07945"
     CDS             complement(9815..10342)
                     /locus_tag="VFQ79_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0139626.1"
                     /translation="MKQLIVFSGPPASGKSLIGGMLARRFGIVHFEMDRIRVALMPDS
                     AHTRQDRIVAYRAMHAAAIAVLDSGSSVIVNAGYSHTGEREELRRIASGHDAQLVLIE
                     CAVPVEIAVERCRARAATHPGLDLTGERVRELVNGFPYTHAGLLLDSTLPADRLLDLV
                     EDYLESSANLPFRYR"
     gene            complement(10339..>12465)
                     /locus_tag="VFQ79_07950"
     CDS             complement(10339..>12465)
                     /locus_tag="VFQ79_07950"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU0139627.1"
                     /translation="YRPLPQTSGELAAPVSSRVSIVRDSLGVPHISAANIEDAIFAQG
                     FVTAQDRMFQMDALRRLATGELAEIVGPVAADSDIEARRLRLRRIAAQHAAALKPEER
                     RYFSAYARGVNYYIETHRSRLPVEFSLLRYDPRPWTVADSIAAGLQMFRTLTTSWKRD
                     LAKEAMLRDGNPDKVNTLFRTGPGSDEVSGSNAWAVAGRHTQTGKPVLASDPHLELTL
                     PSTWYMNHLRAPGMDVTGVSLPGLPAVIIGHNARIAWGVTNLQFDVQDLYIEKLDPAS
                     GRYLFDGKVEQARLEREIVRVRGAASVPLAVWVTRHGPIFVSDRNRFLALRWSAAEPG
                     GFAFPFFDLNRASEWQEFRTALSTYPGPAQTFVYADVDGNIGWQAAGKLPVRSGFQGD
                     VPLDGSSGTNEWTAFIPYDELPSSFNPPSGIVVSANQNSFPPDYKYPVQGNFASGHRA
                     ALIRKMLAAGKVDPAGMLVIQKDVYSPFLHYLAQEVVAAYSRAGNKSERADDAAALLK
                     NWNGQMEKGNAAPLIVQLTYEHLSRALAASAAPQNALAYEHAGTPGVVEEIVRNRPQG
                     WFEDYDRLLAGTFKEAVDEGARMQGNDPRKWDYGRYIEVRLVRPVLSRVPWVGRYFNI
                     GPAPMSGSSTTIKQTTRRVAPSMRMVADLANWDNSLQNITIGQSGQILSSHYSDQWKA
                     YYTGQSFPMQFNHVAADSTLVLEPSR"
BASE COUNT         2573 a         3739 c         3653 g         2500 t
ORIGIN      
        1 aggctcgcaa gcggcaggat ggctccgcga aaccgccaca gttgatccgc aacgaattcg
       61 gcggctccgc cggcggcccc gtccttttgc cgaaaattta cgacggcagg gaccgcacgt
      121 tctggttttt cacctacgaa gggaaccgca ccatcaaccc ttctacgaag ggctaccagg
      181 ttcctaccga ggccatgcgc aacggcgact tcaccggcct ggttgatgct tcgggcaggc
      241 aatacgcaat atacgacccc tataccacga atccggcgac cggcgaaaag cagcagatct
      301 cctaccaggg ccggctgaat gtgatcgacc ccgccaggat gagccctttg gcgaagtatc
      361 tgtacagcat cactccgctt ccgactgatc cttccgtcaa tccgctgatc gacaacaact
      421 ggtggggccc ggcgatcaac acgacccgca actggacgac gactgcgcgc gtcgatcacc
      481 gtttctccga ctacgatcag ttctacgccc ggtatacgca gggcgatttg ttccgcataa
      541 acgatttcta cggccaaccc atgctggaca acgtagccgg caccaatcgc acgctggctc
      601 cgaacaagag cctggcgacg tcgtacctgc acaccttcag ccccaccctc ttcaacgagc
      661 tgatggtcag cgtctctcgc gagaacgcgt tttccggcac gggtgagccc ggcgtctatt
      721 acaacgatat gctgggattg ccgaatccga tgggtgtccc cggctggccg ggcatctatg
      781 acacgggtct cagttcctat tatttcgaga cgcagaatac aaaccgtgcc gcatttacca
      841 actatatcct cgataacaac gccaccaaga tttttggccg tcacgagttt cagtttggcg
      901 ggcatctccg gtacgaccag ctaaactatc tgccggagca gcagcacccg cagggcaacc
      961 acaacttcaa ctcgcgggca acggcgctgg tcgatccggc cggcgcgtcg acaaacccgc
     1021 agccgttgcc ctataccggg agcaatctcg ccaatatgta tctgggcttg atgaattatt
     1081 ccaaccagtt tgtccgcgga tatttttatg cacgcgcgaa agagtatgct ctctatttcc
     1141 aggacaactt ccgtgtcaac cagcggctga ccctgaatat tggtttgcgc tgggagaaat
     1201 ggccagccta ttacgaaaaa aataacatga tgaccagctt cgatcccgcc agtaacagga
     1261 tcgtactggg tcaggacctg gacaccatgt acaggctggg cggctcggtt ccgtccatcg
     1321 tcaaccggtt ggagacgctc ggcgcaaagt tcgcgaccta taaagaagcc ggcatgccga
     1381 agcacctgat gagtagcggc tggcgcgatt tcggacctcg tctcggcttc gcctaccgcg
     1441 ccggcgacgg caccggttcg tttgttctcc gcggcggtta ccgcgtttct tatttcccga
     1501 ttccgctggc tccgtggggc cagagaatgc gccaaaatgc tcccttgacg gcgcgtttcg
     1561 ttacttctca gaccgacgcg gcgcagtcgc cggacggcct gccgaactgg ggcatgcgtt
     1621 ccgtgccgac agtggtcgcc ggtgtcaaca gcaaggatgc cgtgcagttg accaacgctt
     1681 ccctgatcac acgcggacag gcggtggtaa gctacttcag tccggaccag cccgatagca
     1741 aggttcatga ctggaacttc acgattgaga aggaagtcgc aaaaaatacg gtagcccgcg
     1801 tcgcttacct cggcaaccat gtcagcaatc tcgagcaata tttccgcttc aacgaggcga
     1861 caccggagta tatctggtac gccacaacgg gcaccagact gcccaccggt gaattcgcca
     1921 gcgtggcgcg gcggcctttc aaccaggagg tgtacggcac gatggaagag taccgcaaga
     1981 gtggctggtc caacttccag ggtgcgcagt tcgagttgga gcgccgctac agccagggct
     2041 tcgcttacca gatcttctac aacttcggca acgccttcac cgccggtggc aagagctata
     2101 gcggtgtcgt gccgaacacg aacgtgtttc tgcccggcgc cgtgccccag gattacgacg
     2161 agcgcaatcg tcttttcaat taccagcgcg acgtgaccat tccgaagcat cgcctgcggt
     2221 ggaactgggt ggccgatctg ccgtttggca aaggcaagaa gttcggcggc aatgccggtg
     2281 gcgtcctgga caaagtcatt ggcgggtggc agatcaccgg tatgggctcg ttgtggagct
     2341 cgtattggca aatgccgacc acggtctatc cgaacggcaa caatgttgaa gtttacggct
     2401 acgactatcc cattcaggat tgccgcagcg gcgcctgccg gcccggctat ctctactgga
     2461 acggctacat caacccgaac cagatcaaca gctacgacgc caatggcaaa ccgaacggtg
     2521 tgatgggtgt ccccgacaac tacaaaccgg ccggccagcc gttgattccg tggccgctca
     2581 acccgagtac cagcgatccg atgtacgcct attacggttc caacaccgtg tgggtgccat
     2641 tgaacgatgg tacaacccag cgagtcaact tcgatgacgg actgcatccg tggcgccagc
     2701 agtacctgcc gggaccgcgg caatggaacg tggatgcttc catcgtaaaa aacatacggc
     2761 taaccgagag cgtcaatttg cggttcaatg ccgacatctt caacgtcttc aaccacccga
     2821 acaatccggc ctggaccggc agcaatacca tgccggtcgc cgcggacggc gtctacagca
     2881 ccttcacctc cggcctcgaa tcgcgcgaac tgcaattgtc gctacgtttg agctggtagt
     2941 tacttaatcc ttccggggca ggtcttcggg cctgccctta gccgtaccag cgcaccggtt
     3001 cgcgtcttac actggaatca aggtgcgtaa gatcatttgc ttttggacgc tgctttgcct
     3061 gcctgttgct gcgcaacaga agcctctgga gctggtggag gccgtctttg cacaatacga
     3121 ggacgggccg ccggttcctt ccggacacaa gtaccgcgcc ggtgaaacgg tctttatctc
     3181 atttaaagtc gccaactatc gtgtgaacca gaacaaccgg atccatttga aatacacggt
     3241 cgaagccctg gatcccgctg gcctgaaact ggtcccgccg cagtcggatc aggtatccga
     3301 agaactgtcg ccgcaggata agaattggat gccgaaaatc cgctttgatg cgctggttcc
     3361 acccacggcg gcgccgggcg aatacaaaat agccgccacg gtcaccgatg aaatggggag
     3421 cggtcagaaa gccgcgaaag agtttccctt ccaggtggat ggaagtaaca tgcaaccgag
     3481 cgacaccata acggtgcgcg acttcgcttt ctaccgcacc agtgaattca ccgaaccgct
     3541 gcaaatcgcg gcgttcggtc cgagcgacga ggtctgggcg aggttcctga tgaccggcta
     3601 taaattcggg ccggacaatc gcgtgatggt ggagtatggt gtggaactcc ttaaccccga
     3661 agggaaggtt gttttttcgc aatcgaaagc tgccgatttc aacgaggagt ctttctaccc
     3721 caggcgctac gtacccggta cggccagttt ccggctgacc aaagttcagc ctggtgagta
     3781 tacggtccgt ttaacgatac gcgacctggt aggcggccag tccaacgaat cgacgcatcc
     3841 atttcgcgta gagtagtttc agtggaaagc ctgagcccgg tcgaagtcag agttcttggt
     3901 gcgctgattg agaaagagag caccacgccc gaatactatc cgttgtcgtt gaatgcgctg
     3961 gtaaacgcct gcaaccagaa atcgaatcgc gagccggcgg tgaactatga cgaagacacg
     4021 gtgctggctg ctatcgaagc gctgaaggcc cgccgtttcg ccgcggagat cattccttcc
     4081 tccgggtcgc gtgtccacaa gttcgctcaa cgcgtttcgg agcggttgaa tctcggccgc
     4141 cgcgaactcg ccgttctgtg tgtgctgctg ctgcggggtc cccaaacact gggcgaactt
     4201 cgcgaccgga cagaacgcct tcattccttc ggcgatctgg acgaggtggc ttcggttttg
     4261 gaaagactcg ccgagtggga cccgccgcta gcggtgaagt tgccccgcca gccgggcacg
     4321 aaagaacccc gctacgcaca actgctgggc ggcacgccag atgttcaacc ggcatcggcc
     4381 gaggcaccag ttgcagcgca gccgcgcgac gagcgcctcg cgtcgctgga ggcggaaatt
     4441 gctgaattgc gcaaggagat cgaacaattg aagcaacagt tcagcgattt ccggcgccag
     4501 ttcgaatgag cgtggaccgg ctctctccgt ccgaactata gaagagcaac ctgctcctca
     4561 gttggctccg ctcaacagta ccagggctcg tccatgtagg ggatcgtggc gcggggcatc
     4621 aggtcgaaat tctcgggaag cggatcgtcc gtgacaattt gccagatgtt tcggaaaatc
     4681 tcgcgcctgg gtgcgtgccg cctctgctcg tcgtgaacca gcgaaagcac ccgggcggct
     4741 aattgatcga tgtccggatc ctcgtgccgc cagcggtaga tgagcgccgc ttcgtcaaaa
     4801 cgctcgatcc tctcctggat atcgggcagt tccagaaggc gggaacccgt cgtcaccagc
     4861 agccgcaagg caagctgcac gggactgacg ttctccacca atccgagctc ggcaatcacc
     4921 cgcagcaggt cgctatatcc ggaccgtgtg gtccatggtg taaaaggaat gaacgtcggt
     4981 gccatcacca ggccggccgc atccagcaac ccggcggcgc gaatgaaatc ggcacgtgtg
     5041 tggccctttt cgagtttcgc taatacccga tcatccaccg actcgacagc cgtcgtcaca
     5101 aagaggcagc ccgtgtcgcg gagcacgggg agcaggtccg gtgcttgaat cagatgttcg
     5161 attttgatcg tggcgtcata gctcacgccc ggaaattcac ggcacaactc ctcgacaatg
     5221 cggcgtgcgt gagctggccc attcaggaaa tcggggtcac cgaacgtgat gtgctgcgca
     5281 ccggcctcca cctggcgccg gatatccgcc atcacggtat cgtgttgtac aacgcgaaag
     5341 cggccgttgt agaccggcac tacgggacag tgacggcaca ggtgcttgca gccccggctc
     5401 gcttcggtgt agccggcgat tcttgtagcg ccgttgacgt gtagtttcgc gtaacgcgcg
     5461 agcggcggaa ggctgctgcg gtcgggcacg ctgaattcga ggcggtcgag cgagatcaac
     5521 tgctgttgca ccggatattc atcttgcacc agtcgctccg ccaggtccgc cagtgcctgt
     5581 tcgaattccc cgccaaggat ggtctcgacg cccagtttct tcaggtaacc tgcgttgact
     5641 ggcgcgtaca ggccataggc gcataaatgc acttttggat tcagcaaccg gaccttcggg
     5701 atcacttgtg ccgccagacg cgtcgcggta tgcatcggca ggtacagcgc aaccagttgc
     5761 gcttcacgaa tgggaaggga aggcagcgtg gtaatggcaa gatccacgca catgacatcg
     5821 tggccgcgtt ccttcaacca cgcggccggc gaagccagcc cgaatggctg ccggccgagt
     5881 tcataggtgg atatcaaaac aactttcaac gcatagaacc ccaacttcca ttatgggcag
     5941 agcggttcgt cactcgggtg agttcagtcc ccgtgtgatg ccagccagca tttttctcgg
     6001 tgcgatccgg gcggctgcca tcacgatacg gttttgcaga cctgggatga caatcgtctt
     6061 gcccgccatc aagccttcga agccgatgcg cgccacgctc gccgcatcca tcacggagct
     6121 tctcttgaac aatcgcgcgt tcgaagtgcc cgcggtttcc gcgaagcctg tatgcgtggg
     6181 cccggggcaa agtgcggtaa tgctgacgcc tgtgccggac aactcgttcg ccagtgcttc
     6241 agtcagcgag acgacgtaag ccttggatgc atagtagacc gccatggtgg gccccggaac
     6301 gaagccggcg gtggatgcca cgttcaaaat cctgcccgag cgccgggcga tcatgcccgg
     6361 caggaacagg cgggtgagat gagtgagagc ggtcatgttg acctgcatca tctccagcag
     6421 tctaccggta tccgattccg cgaaaaagcc tcgcaagcca aaaccggcgt tatttaccag
     6481 cgcctcgagt gcgatgccca gttttcccag ttcttcatac acccgcggcg gtgcgtccgg
     6541 atccccgaga tccgattgga tcacgcggga tcgggctttg taccggtttg cgagttccgc
     6601 ggccagttcc tccagcttgc ttacgcttct tgctgccagc gccacgctgt atccctgtgc
     6661 ggcgaagatc ttcgccaact cggcaccgat tccgctggag gctccggtaa ccagaaccca
     6721 aggcccttcg tttctcgttg tttcggcttg catgatacgc tgaaaattca agcttatgag
     6781 taatatcagt attaccttgc ccgacggcag caggcaagaa gtgcaggccg gaacgactcc
     6841 gctggatctg gcgaaatcga acagccgccg gctggctgac gacgctgtcg tcgcccgagt
     6901 caacggccaa ctgtacgact tgacccgtcc cctggagagt gacgcgagcg tgcagatcct
     6961 cacttcacgc gacccggaag cgctcgaggt atatcgccat tccacggctc accttctggc
     7021 ggcggctgtg ctggagttgt atcccggcac caaactgggc attggtcctc cgatcgataa
     7081 cgggttttat tacgatttcg aacgggaaac gccttttacg ccggaagacc tggagcgcat
     7141 tgaaaagaag atgtgggagc ttcaggctag cgacctgcca tatgaacgca agctgacacc
     7201 gaaagaagag ggtttgaaaa aatatgccgc catgggcgaa cccatgaaat gcgagctcat
     7261 cgaagagcgc gccgacggca tcttctccga atacaccctg ggaccgcatt tcatcgattt
     7321 ctgccgcggt cctcacgtgc cctccacgtc gagaatcaag gctttcaagc tgctttccat
     7381 cgccggtgct tactggaagg gcaacgagaa aaaccagcaa ctgcaacgca tctacgggac
     7441 cgcgttcttt tcgaaaaaag agctggaaga gtacctgaag caactcgaag aagccaagaa
     7501 gcgcgaccac cgcaaactcg gacaggagct ggacctgttc agtgttcagg agcttgcggg
     7561 accgggcttg attttcttcc acccgaaggg cggcatcgtc cgaaagctgt tggaagactg
     7621 gatgcgcgat cagtatctcg cgcgcggata ttcccttgtc tatactccgc acatcgcgcg
     7681 ttcggacctg tggaagactt cgggccacta caatttctac gcggagaata tgttcaagcg
     7741 catggagttg gacgacgccg aataccagct caagcccatg aattgtccgt tccacattct
     7801 gatctacagg gaccggatgc gcagttaccg cgatcttccg gttcgcctgg gcgaactggg
     7861 aacggtgtac cgctatgaac gttccggcgt gatgcacggc ctgctgcggg ttcgcggctt
     7921 cacccaggac gatgcgcaca tcttctgcac gccccagcaa attgaagacg aagtcgtgaa
     7981 ctgtctccaa ttcgcgctgg acgtcctctc tactttcgga ttcgacaagt atgaagtgga
     8041 gctgtccacc tgggatggcg gcgccagcgg gaagtacgac ggcactccgg accagtggca
     8101 gctcggcgaa aacgcactgc ggaaagcgac cgaacggctt gatatgcagg tcaaactgag
     8161 cccggacgaa gctgctttct acggaccgaa aatcgacgta aagctggtgg atgccatcgg
     8221 acgtctgtgg cagctctcca cggtgcagtt cgattttacg ctgccgcggc gcttcgggct
     8281 cgaatacatc gcggaggacg gcaaagccca tcagccgctg atgtgccatc gcgcgctcta
     8341 cggctccgtc gagcgattct tcggaatcct tctggagcac tacgccggtg cgttccccgt
     8401 gtggctcgcg cccgtgcaag cggcggtgct tcccatcacc gaccgccaac tcgaatatgc
     8461 gcggcaggtt acggaacagt tgagagccgc gggtataaga cctcacctgg acgatcgcaa
     8521 cgagaaggtg aacttcaaaa tccgcgaagc gcagttgcag aaggtgccgt atatgctggt
     8581 ggtcggcgat cgcgaagccg aaaacggcac cgtctcggtg cggaaccgta agcatggcga
     8641 ccagggcgcg aaaacgctgg acgcctttct cgcggaggtc aagaaactga tcgacacgaa
     8701 agctccgtcc gagtaggcag atggcggtgg cgctcgcgct gttgctggcc gccgtggccg
     8761 ccggatcgct ggtgtactgt atcctcaccg tgattgcggt ttcacggtat ctcgccgtcg
     8821 cgccgccgcc gctttccagc gcggagccgg tcagcatcct gaagccgctt tccggcgctg
     8881 acgaagggct cgaagaaaac ctgcgcagct tcttccgtca gaaatatccc catttcgaaa
     8941 ttttgttggc tgttcgctcc gaatccgatg cggcggtgaa actcgtgcgc ggattgcagg
     9001 cggagtatcc ggagattccc gcacggcttc tgattaccgg cgaaccgccg tacccgaatg
     9061 cgaaagtctt cagcctcgat cgcatgatgg ccgaagcccg ctacgatttg ctggtgatga
     9121 gcgacagcga tattcgggta acgcccgaac tgctccaaac gatcgcgggt gagttccagg
     9181 attccaacct cggcgtcgct acctgtcctt atcgggccgt gccgggttcc agcatctggt
     9241 cgcgattgga agcgatcggg atgaatacgg aatttctcgg cggtgtgctg gtagcacgga
     9301 tgattgaggg aatgaagttt gccgtcggtc ccacgattgt tgctcgcaag cgggtgattg
     9361 aaaaaatcgg cggcttcgcg cggctgaggg aatatctcgc ggaagatttc gtcatgggga
     9421 agtttgccgc cgatgccggt tacggtgtgt cgctctcctc ctacgtgatc gagcatcgaa
     9481 tcggcaccga aggtctgcgg gctaattttg cacatcgcct gcgttgggcg cgaagcacga
     9541 gacgatccag acccgcgggc tacgtaggac aaattttcac gaatcctctg cctgtggccg
     9601 taatccttgt ggctcttgaa cccgcatggt ggccggcgct tccggttttg gcggcggcgc
     9661 gtgcagcagc cgcctgggcc gtttcccagc gagtgctcaa ggacccgctg acgcgcgcgc
     9721 attggtggct ggttccggtg caggacttcc tgagctttgt gttctgggtt gcgggcttct
     9781 tcggcaattc cattcagtgg cgggggcgcc gctattacct gtatcggaac ggcaaattcg
     9841 cgctgctttc caagtaatct tccaccagat ccaacaggcg atctgcgggc agcgtactgt
     9901 ccagaagaag ccccgcgtgc gtatacggaa aaccgtttac cagttcgcgc acgcgttcgc
     9961 cggtgaggtc gagccccggg tgagttgcgg cgcgcgcacg gcagcgctcg actgcaatct
    10021 cgacgggaac cgcgcactcg ataagcacga gttgagcatc gtgcccggag gcgatacgcc
    10081 ggagctcttc ccgctcgccg gtgtggctat agcctgcatt tacgatcacg ctgctgccgc
    10141 tatccaacac cgctatagcg gcggcgtgca tcgcgcgata ggccacgatg cggtcctggc
    10201 gcgtgtgcgc ggaatccggc atcagggcca cgcggatgcg gtccatttca aagtgcacga
    10261 tgccgaaccg gcgggccagc atcccgccga ttaaagactt gccggacgcc ggcggtccgg
    10321 agaagacgat cagttgtttc accgggaagg ctccagcacc agagtcgaat cggcagcaac
    10381 atggttgaat tgcatgggaa agctttggcc ggtgtaatat gctttccatt gatcggaata
    10441 atggctggac aggatctgtc ccgattgtcc aatcgtgatg ttctgaagcg agttatccca
    10501 gtttgccagg tccgccacca tccgcatgga aggcgcgacg cggcgcgtcg tctgcttgat
    10561 cgttgtagag gatccgctca tgggtgccgg tccgatattg aagtagcgcc ccacccacgg
    10621 cacgcggctg aggactgggc gcaccaaccg cacctcgatg tagcgtccgt agtcccactt
    10681 gcgcggatcg tttccctgca tgcgcgcgcc ctcatcgacc gcctctttaa acgtgccggc
    10741 gagcaggcga tcgtagtcct caaaccagcc ttgcgggcgg ttccgaacga tctcttccac
    10801 tactcccggc gtacccgcat gctcgtacgc cagggcgttc tgaggtgccg cgctcgcagc
    10861 cagagccctg ctcagatgtt cgtaagtgag ctggacgatc agcggcgcgg cattgccttt
    10921 ttccatctgc ccgttccagt tcttcagcag cgcggcggcg tcgtccgctc gctcgctctt
    10981 gttgcccgcg cggctgtacg cagccactac ttcttgcgcc aagtaatgca ggaatggcga
    11041 gtacacatct ttctgaatga ccagcatgcc ggcgggatcc acttttccgg cagccagcat
    11101 tttgcgaatc agggcagcgc gatgacccga tgcaaagttc ccttgaacgg ggtacttata
    11161 gtccggaggg aaggagttct gatttgcgct tacaacaatt cctgatggtg ggttgaagga
    11221 ggaaggcagc tcgtcgtaag gaataaaagc cgtccattcg tttgttcccg aactgccatc
    11281 cagcggaaca tctccctgga atccgctccg gaccggcagc tttccggcgg cttgccatcc
    11341 gatgttgccg tccacatcgg catagacaaa tgtttgcgca ggtccgggat aggttgaaag
    11401 cgcggtgcga aactcctgcc attcggacgc gcggttgaga tcgaagaatg gaaacgcaaa
    11461 accaccgggc tccgccgcgc tccatcgcaa tgcaagaaag cggttccggt cggagacaaa
    11521 gatgggcccg tggcgcgtca cccatacagc cagcggcacg ctagccgctc cgcgcacgcg
    11581 gacgatctcc cgctcaagcc gcgcctgctc caccttgcca tcgaagagat agcgcccgct
    11641 ggcggggtcg agcttctcga tatacaggtc ctggacgtcg aactggaggt tcgtaacgcc
    11701 ccatgcgata cgcgcgttgt gtccaatgat gaccgcggga aggccgggca acgagacgcc
    11761 cgttacgtcc attcccggcg cgcgaagatg attcatgtac cacgtcgacg gcagcgtcag
    11821 ctccagatga ggatcgcttg ccaataccgg cttgcccgtc tgagtatgcc gtcccgccac
    11881 cgcccaggcg ttggaaccag aaacctcgtc cgatcccgga ccggtccgga acagcgtgtt
    11941 cactttgtca ggattgccat cacgcagcat cgcctccttc gcaagatccc gcttccacga
    12001 agtggtgagt gtgcggaaca tctgaaggcc ggcggcgatg gaatccgcaa cggtccacgg
    12061 ccgtgggtcg taacggagca gggaaaactc cacggggagc cgtgagcgat gcgtctcgat
    12121 gtagtagttc actccccgcg cgtaggcaga gaagtagcgc ctctcttccg gcttcagcgc
    12181 ggcggcatgc tgcgcggcga tgcgccgcaa gcggagtctc ctggcttcga tatccgagtc
    12241 ggcggccaca ggaccgacga tttccgccag ctcaccggtg gcgagccgcc tcagcgcatc
    12301 catctgaaac atgcgatcct gtgccgttac gaagccctgt gcaaagatcg cgtcttcgat
    12361 gtttgccgcc gagatgtgcg gtacgcctag cgaatcgcga acgatggata cacgcgagga
    12421 aaccggtgcg gccagttctc cggacgtttg cggcaacggc cggta
//
LOCUS       DASTMK010000186        25452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_297644, whole genome shotgun sequence.
ACCESSION   DASTMK010000186 DASTMK010000000
VERSION     DASTMK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 25452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25452
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297644"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFQ79_07955"
     CDS             complement(<1..364)
                     /locus_tag="VFQ79_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0139628.1"
                     /translation="MNPAEPTDFPLGRWNLYIGGAGRTVQGYINIDLLAIPGVDVAAD
                     AEQLPFPADAFQRVECDAVLEHVRNPERVLSEIRRVLVPGGYVHLVTPFCHPFHEYPK
                     DFRRFTIDGLKELAGPLDP"
     gene            complement(456..1364)
                     /locus_tag="VFQ79_07960"
     CDS             complement(456..1364)
                     /locus_tag="VFQ79_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU0139629.1"
                     /translation="MTVMHSEKGKPISDLADRHELITDIFRSPQTAAEWDQYRLTREQ
                     VAFFHEHGYLAGIRVLTEEQADRILPEIDEAMNPSSPGIELFNQYYSNESEDSSRILF
                     HALGGWRFRPGLHDLLWNPAFLVPAMQLLGDSVRFWHDQLFCKPAHHGGVVAWHQDYS
                     YWTRTQPMAHLTCWIGLDDSTIDNGCVHYVPGSHRWPLLPITGLAGDMDEIRTVLSPE
                     QEAQFHPVPVQLKRGEASFHHPLMVHGSYENRTGSPRRAAVINVFRDGVHSASDAEPL
                     PGVPPIPTGKKMEGKFFPLLIDARNA"
     gene            1445..2638
                     /locus_tag="VFQ79_07965"
     CDS             1445..2638
                     /locus_tag="VFQ79_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0139630.1"
                     /translation="MTGRERIARTMRHHEPERVPVMCQLSIGHMNLNGGYKPHEIWYD
                     TEAFADASVKLARRYGFDGILVVLTGRPPDYLDNVQSIREDSDGEWITWRNGDRTFLP
                     WDDMPQHYPCDTSAPVRAVFETFDPERDILRLDHYLGYVWNVLYHIQEVPGKPDAGPL
                     RSGSVPEYLLRAFDLVRSRVGAELSVHGSIYSPLTHYFELFGYETALLTFVDDPGRAH
                     AILERLSDGVIAWSLALLARGADALDHSSAFVAAPFLSRKLYEEFVVPYEARVNEAIR
                     AAGGIVYTHTCGRIADRLDLIEATRTMGIDTLDPPPLGNCDLAQAKRAVGERLFLKGN
                     MNSVALLDYKTPEQVRLEAVRALEAGKPGAGYILSTACSVAPRVEPWKLEMLAPIANE
                     LGQYE"
     gene            2660..2806
                     /locus_tag="VFQ79_07970"
     CDS             2660..2806
                     /locus_tag="VFQ79_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139631.1"
                     /translation="MRFWYLFWVVTFAISAVAFAAIAAVVAVRGVGDLRQMLAALKDG
                     ADKS"
     gene            2803..4233
                     /locus_tag="VFQ79_07975"
     CDS             2803..4233
                     /locus_tag="VFQ79_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU0139632.1"
                     /translation="MNFTPLDWVIVLVYLAVTMAAGLAGKKYVLGVSDFLVAGRKIGP
                     FVGIATLAATEIGTITFMYYAELGYKTGFASFINGLIAGCVMIFLGRTGFMVHKLREL
                     RIMTVPEFFELKYSRRLRVLTGVLVSIGGILNMGVFLRVEGTFLTIIAGLPLDYLQIT
                     MTVILLLEIVYTVLGGMVSIVITDFIQFVMLSIGTVLVTVYAMTVAGWGNMFDAVAGT
                     MGEPGFNPLTNPEYGWAYILFQILLWMSVDTCWQTTAMRTFSTRDPETSKKVFFWTGF
                     IYLGRGMMPMLWGIAALALLGPQQDSLHAMPQMIARILPAGILGLVVAGMLAATMSVN
                     SSYLLGWSSVIAQDVIAPLRKTPMSPRRQIVVNRITNLLVSLFVLFWGVWYTLPGPVW
                     FYLNITGTIFLAGAFSAFIGGLFWKKANVVGGYAAMLAGASGSIAFFFFKVPVSYAGL
                     GSFALAAVGMVGGSLIGDRMRKRSAA"
     gene            complement(4264..4440)
                     /locus_tag="VFQ79_07980"
     CDS             complement(4264..4440)
                     /locus_tag="VFQ79_07980"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEU0139633.1"
                     /translation="MPVAESVWAIPSTARSKQLTVRLDADVVAWLRRQGRGYRTRLNQ
                     VLREAMLEDIKKSA"
     gene            4417..5033
                     /locus_tag="VFQ79_07985"
                     /pseudo
     CDS             4417..5033
                     /locus_tag="VFQ79_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(5046..5966)
                     /locus_tag="VFQ79_07990"
     CDS             complement(5046..5966)
                     /locus_tag="VFQ79_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139634.1"
                     /translation="METNDNSRWVAERLLKVEPAWTPTVAQARVTLNRKLRPEPQRKA
                     WIAASALAGLALAILALPQGRALAQELWFRLFLNRIDVVRVDLSDAPFDTDFTGSGGQ
                     RVVAGLSEAEAQAGFRPRLPVFDSWPGPPAISVIGPMTMRQTIRTSELRSALARIGAS
                     DVVVPDEWDQLTMRASMGPLVVADYPGEIQILQAKPVQLFLPTGFPLSQFAETAFRSL
                     GLSWWEARALARRYVAQPSLLIGIPTDEAARIEEVSLRWGSGLLVEEFKDDGETERVT
                     LVFGTKNGIYAVMSPSREVCLRVADLLPSN"
     gene            complement(5950..6468)
                     /locus_tag="VFQ79_07995"
     CDS             complement(5950..6468)
                     /locus_tag="VFQ79_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0139635.1"
                     /translation="MSRAGTTGQMDLDTLFNAHYGRIARVIGRVVRDQARAEELAVEV
                     FVKWQRNPRAQGEQAEGWLYRTAVREAIDEWRKQARRERFERLFSSLRGSPPTPQELY
                     EADVEQTNVRAALAAIDPRHAQVLLLWAEELTYREIAAAADINLTSVGSLLSRAQTAF
                     RKEYERRYGNKR"
     gene            6697..7584
                     /locus_tag="VFQ79_08000"
     CDS             6697..7584
                     /locus_tag="VFQ79_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0139636.1"
                     /translation="MRRFLLRATRRLLKEIDLHLERDGDSAGRVIAAGNGRETGIERF
                     LRAAILPDVARQYLDHHLPRLIRTFTLLPPNGGSALELGSYVYGAAVLERVLGYRAVR
                     GAYYGAQPGSDRKTLEIAGQPVFELDIDLFDAETHSYPYPDGTFDAVLCCEVIEHLLR
                     DPMYLLFECRRVLKEGGLLLLTTPNAASLTSVARTLHGRKSPQLFSAYPAPGNADTPH
                     VREYTVSELAAVVTAGGFHTEVIFTEVLEGFENTSWVKPLLEREGFDTAMRGEQAYCL
                     ARKQSTLPCERYPRWLYAI"
     gene            complement(7787..10465)
                     /locus_tag="VFQ79_08005"
     CDS             complement(7787..10465)
                     /locus_tag="VFQ79_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139637.1"
                     /translation="MRSAFRRLSALLAGTCAERDLEDEVRFHLDTLAEEYQRKGMPQV
                     EARAAARRDFGGVARMKEAYREQRGVPLIETTLQDLRYGLRGLWRSPGFTLAAMISLA
                     LGIGANTAIFSLFNTLMLRMLPVREPEQIVSLYRVGGWGRGFTSYPLYQELLKQTDLF
                     EGVLARSAPWRVRYTTAGSTEQPDFLFREFVSANYFEVLGVQPALGRLFAGEESSPQA
                     QPYAVLNYDFWQARFYGDPNVPGKQIVIDDQPLTIIGVARKGFRGVQVEGQADVWVPI
                     TMHRARFQDAGMHWLWLLARLQPGVQGEEAQAKVNVLVRNFLNSNYANDPNTPWQRKR
                     MGQRLELRAGGTGVSFLRDQFQKPLLLLMALVTLILLIACSNVANLLLARGTARRREI
                     ALRLSLGASRLRLVRQWLTETSLLAAVGIPAGIALGIWGSRNIVQFLPVDDANLTLDV
                     KPDANVILFTVTVSLAAMFLFGLLPALVSTNVPPGAVLKEGSAGSGRGCRHRVRKTLV
                     VTQVALCIVLVSGATLFSRSLFALNTTDTGFHHYDVLTFQFDSPRSYKSSQVASIRER
                     LVQRIRAIPGVTAVSYGFPGPYQGGTWSAGIRVPGSERTAVEPASVELQSVAARYFET
                     IGSLPVRGRDFQDSGAEQMRKVAIVNEAFSREYLNGADPVGHTLSFDDKEPQGGIPIY
                     IVGQVRNMLHQGVREHPVPTVYLPVTQMESTSDPILLVRAGVSTGTLVPLLRRELAAL
                     DRAVTLSGVRTVRQRFEDSIFLDRLIASVSVLFAILSLVLAGVGLYGVMSYLVAQRTA
                     EIGIRVALGAAPGRMLWLVLRDGLILIFAGAAIGIPLALIGARLAAGLLFGVKPADPA
                     AFAASAVFMLLIGLAAAAVPGLRAASVDPMQTLRRE"
     gene            complement(10465..10815)
                     /locus_tag="VFQ79_08010"
     CDS             complement(10465..10815)
                     /locus_tag="VFQ79_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139638.1"
                     /translation="MSKQESQPEVLQGTLDLMVLRTLETMGPQHGLGLAKRIEQISEG
                     TLSLNQGTLYPALLRLEQRGWIRSKWGTSDNNRRARFYSLTAAGRRQIAEEQAGWTRI
                     VSLMSRFLRTGEEG"
     gene            complement(10882..11301)
                     /locus_tag="VFQ79_08015"
     CDS             complement(10882..11301)
                     /locus_tag="VFQ79_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0139639.1"
                     /translation="MNAKTFVDTNILIYAHDADAKAKHEIAKGILRELWERRTGVLST
                     QVLHEFYVNVTRKIASPLPKSSARAVVDSYAVWCVDTTLAEISAAFRIEDEACIGFWD
                     ALIIAAAWKAGAGRILSEDLNAGQTIAGVRIENPFSR"
     gene            complement(11291..11560)
                     /locus_tag="VFQ79_08020"
     CDS             complement(11291..11560)
                     /locus_tag="VFQ79_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139640.1"
                     /translation="MAKKEDMARENIKKQNVTISISPDIVRKAKILAARRSTSISGLL
                     AEQIEILVGADEAYERSERSALALLEEGFHLGGFPPASRDELHER"
     gene            11669..12172
                     /locus_tag="VFQ79_08025"
     CDS             11669..12172
                     /locus_tag="VFQ79_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0139641.1"
                     /translation="MTDAAILAAWYGHKDTIYRFAWRMTGSASIAEDVLQEVFLALLR
                     VPDHFDVRRAPLRAFLLAVARNQVRKRWEKERRWDTLDDDMFEAAPMNLLAAEIADSV
                     ARAVQSLPPRQRETLVLFEYEGLSLEEVARAVDAEVGAVKSRLNRARENLRLILAPLR
                     GAYGAFK"
     gene            12156..12467
                     /locus_tag="VFQ79_08030"
     CDS             12156..12467
                     /locus_tag="VFQ79_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139642.1"
                     /translation="MEPLNDHELQTLLKAWDAPEAPEHLQPPLPRRRWYAVLWGASVH
                     IPVPVLALILMAIIALQVFPRRERTPIPPDALREIRLSDFEPVPEAKPVVVRRTHYEN
                     H"
     gene            12454..13317
                     /locus_tag="VFQ79_08035"
     CDS             12454..13317
                     /locus_tag="VFQ79_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139643.1"
                     /translation="MRIISLLLLSATLSPAYQVISSGSGSKDGVGFRYETRVEPRIEG
                     QKVIGFSGGGIYIRDTFHRYLRDDATRRYVGYDLILNKRTDGMLVVTFGQLSVSGEKL
                     GLKGEWPQVPLRVNSTPQTIKPGDEIAIDLFTNPATGQKILDHLVFGEAPEVVRLPEG
                     SPRDFTMDDVPLTLTSPKILLNGKQLESMEAAISGEAVYFYLPGHGRYAMSLAPNLEL
                     GFRKLDEVRGSRISFRNGNDTWTIDCTHPVAPGGGVFNLYVYPDPVWQPSRGADTYQL
                     GASSARAMIRK"
     gene            complement(13450..13698)
                     /locus_tag="VFQ79_08040"
     CDS             complement(13450..13698)
                     /locus_tag="VFQ79_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139644.1"
                     /translation="MPSTSIRIDEQALAVLRDLARRQRQPVQTVLKQAINSYRRRKFI
                     KEANAAFAALRNNPEAWTGEQQERDLWDRTSGDGLKRE"
     gene            complement(13722..14606)
                     /locus_tag="VFQ79_08045"
     CDS             complement(13722..14606)
                     /locus_tag="VFQ79_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0139645.1"
                     /translation="MNELTSAPAFAVQPSPDGSNDASLVELWLSLKVSSHTRRAYAAD
                     VERFLMFVAKPLGWVTLADVQAYAAALEQGSLKPASQNRALTAVKSLLSFGQETGYLP
                     FNVGAAVKLRPNRDSLAQRILEESEVAKLIGAAPEGRDRVLLKLLYVSGVRVSELCGL
                     KWCDALPRTEGGQITVFGKRGKTRTILLKPKFWSQLLAIRGEAGPVDPVFRSRKGGGH
                     LDASQVRRIVYAAAKKAGLEQKVSPHWMRHAHASHALDRRAPIHLVQATLGHASVSTT
                     GRYLHARPTESSSFYLPD"
     gene            14681..14923
                     /locus_tag="VFQ79_08050"
     CDS             14681..14923
                     /locus_tag="VFQ79_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139646.1"
                     /translation="MASRKFTFTLPQDLATEFLHRVPASSRSQYAAAAIAAKLREREQ
                     QLALACEVANNSADVREIETSFDALADEADTVQEPW"
     gene            15453..15797
                     /locus_tag="VFQ79_08055"
     CDS             15453..15797
                     /locus_tag="VFQ79_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139647.1"
                     /translation="MHRIGAIRVSRELAVEQCDLIRWLEQMLSDPSVAIEQRRHERVI
                     SRIVELKAETAARAVRIVLPDPAPSVDFPEGVSLQPGSLTVSFENEQQLLERLFLLAR
                     VLATQPQILNNI"
     gene            complement(15814..16548)
                     /locus_tag="VFQ79_08060"
     CDS             complement(15814..16548)
                     /locus_tag="VFQ79_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139648.1"
                     /translation="MAKRKRQTSEDFDVTEFLRPTDADMRSAVSFLLRGPQVVEVPRP
                     AESGTPASGIAEEGGPVPEETSSSTEQMAPEAGIPVLKIPESSVPDKEGIDIAIPERG
                     IPASGVTPHNVAVTQIALLRRIRQAFRAQDGHSLGEQAVYQALWDHGVPRDPHSRIVT
                     IGYRTLSAVCGLTANNCKANLQGLRDKLAIEETERHTPSAGTTYLVYSADAILKRRQS
                     AGLTHYIKNRGVVFVDPLTGNPIRHT"
     gene            complement(16520..17308)
                     /locus_tag="VFQ79_08065"
     CDS             complement(16520..17308)
                     /locus_tag="VFQ79_08065"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU0139649.1"
                     /translation="MIRVVVANQRGGVGKTTCAVNYAWYLAGKGHRTLLIDTDSQGSI
                     SVMLNLKPRNFFNQFLIHKFHLDECTVKVTDKLDILCGNKDTIQAESAMAGAVVRELA
                     LKIMMEPYESRYDAVVIDVAPSINLLQSCAMVYARNVVIPVDMDLLSLNGANAAYDMM
                     RLLNDLIKANIRAIGLMPCQVDQRLSVTALVQQSLQKMSEMFEVPILPAIRTDQTVNK
                     AFRARKALLDYDPNSKAAQDYLAAFAQITAMIEASDGQTQTANV"
     gene            complement(17305..17493)
                     /locus_tag="VFQ79_08070"
     CDS             complement(17305..17493)
                     /locus_tag="VFQ79_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0139650.1"
                     /translation="MRRHYLKTAQVAESLGISLATLYRWLKESKIPEPYRNPSNNYRL
                     WTVEDVERIRRQAVPEVR"
     gene            complement(17564..18715)
                     /locus_tag="VFQ79_08075"
     CDS             complement(17564..18715)
                     /locus_tag="VFQ79_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0139651.1"
                     /translation="MNDLAIGTFLLSAIFIVYVLMGYPVLLALLSRRGASIRKKPSHH
                     FSVSVLLPVHNGELWIQQKLESIQALKYPRELVEIIVVSDGSTDRTDEIVKQFESARI
                     RFYRIPRSGKACALNTAVQHATGDILFFTDVRQQLEPSSLAHLVSCFSDPQVGVASGE
                     LIIRKGETLGELNVGLYWRYEKWIRKHLSRVDSVIGASGCIYAMRRELAAPLSPDTLL
                     DDVQLPLGAFFRGYRIILDESAKAFDYPTSLKAEFHRKVRTQAGVYQTIRSFPALLGP
                     SNRMWFHFISHKVGRLLLPFALLLLLASSFGLPPVWKWAALSAQAGFYLVALLNPLIP
                     EGSLIKRITSPCWTFVVLIAAALCGISVLFIPSRKLWKQTEVRQSSSAA"
     gene            complement(18964..20088)
                     /locus_tag="VFQ79_08080"
     CDS             complement(18964..20088)
                     /locus_tag="VFQ79_08080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0139652.1"
                     /translation="MLIPEWPIATDRELESLKEVLCSRQWGGFHEVVERFERTFAEFQ
                     HCKYGVTAVNGTVTLEMALQAAGVKEGDEVIVPAISFISTATAVSRVGGIPVFVDIGP
                     LSFQMDPECAARAITSKTRAIIPVHFGGPLADMDQLPRLAAGHGLALIEDAAHAHGSE
                     WRGRRAGSFGLAGSFSFQNSKVMTAGEGGILLSNDAGFDEQVRSFANQGRRQDQGWFH
                     HYTLGTNYRITAFQAAVLLAQLERLPEQISLRTRNAQFLRSDLADVKGLQFQEIAPEV
                     SSNSWYLFLGRVDQKKLGVSRDDFCRSLANAGVPCTPFYPHPLYGNPLYRAGGCRVEP
                     CPNAEARIEDAFWLPHRALMSNAEVAGQIAEAIRKSCQKN"
     gene            20279..21616
                     /locus_tag="VFQ79_08085"
     CDS             20279..21616
                     /locus_tag="VFQ79_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139653.1"
                     /translation="MRYRLAALISILWAVSAVAQVMPSGGPSILSRQPSLRTGPADAL
                     FTIRPSASIVGRYDHGLFLPTTDAEGRLSGQDSYGIAAVLGLDGFHQTRNSVVSLDYR
                     GQFRHYTANDYLDGSDHALSLGYERRLTNRTLVAGTAFGGLYSRSFGVPGLGRMSPPI
                     VDIGPEIIPTNEIFDSRVYYAGAGANIVHQKSMRLSFGAGANGFMVHRRSRSLAGVIG
                     TSAQGNVAYRLSRRHTVGADYTFTKFDFTRGFGISDMHIVSLNYAAQLTRRWTFSARA
                     GIFRFENLRVVTVQLDPVIAEILGRRTGLETQHQVNYGPIVRAVLTREFQRGSFNASY
                     SRIVSPGNGIYLSSIAESARVGYNYRGSRRWNSGVAAGVTQYGALTQTIGKYHNLYGS
                     AQTSYRLKRFLHLTGSFIVRSHGIGEGSDFKRTSYSASIGLAFAPGEIPLSLN"
     gene            complement(21647..22144)
                     /locus_tag="VFQ79_08090"
     CDS             complement(21647..22144)
                     /locus_tag="VFQ79_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0139654.1"
                     /translation="MEIAQLLNEIEPKVDPEEKPLPPDGKKRRTRNSRNAGVRQGGGF
                     AMRTELYRRFGVDVLQIPGLEQSGLSLLTELGPDLSRFPTVGQFVSWLALCPDNDKSG
                     NRWWRGSRDVNNRAGQIFRQCAHSLHRNQSQLGVYLRRMKSRLGPQGATIAPARKIAT
                     IFGSG"
     gene            complement(22178..22438)
                     /locus_tag="VFQ79_08095"
     CDS             complement(22178..22438)
                     /locus_tag="VFQ79_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139655.1"
                     /translation="MAAQHVQHMQKALTQMNVQIHRVINDIAGLAELAIVDAILDGER
                     DPAKLAELRHYRIQADTETIRKSRVGNWRREHLFTLRQSREA"
     gene            complement(22444..22863)
                     /locus_tag="VFQ79_08100"
     CDS             complement(22444..22863)
                     /locus_tag="VFQ79_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0139656.1"
                     /translation="MCLTDRPMIEGSAAGVDIGAREIYAAVPPGRDQNPVRVFATFTE
                     DLNALADWLEACRIRTVAMESTGVYWVPLYEILDRRGLQPCLVNARHMKNVPGRRTDW
                     HDCQWIQYLHSVGCCAAPSGRTMKSVPCAPSCGTGAN"
     gene            complement(23414..25348)
                     /locus_tag="VFQ79_08105"
     CDS             complement(23414..25348)
                     /locus_tag="VFQ79_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139657.1"
                     /translation="MLRYCYLFLLVVPAFADQHFAFDYRRELLGSPPSLSIAVTAADS
                     ATGVVVINGGDTRQPSTPFTFDWGDGQTTSGFFPQQHRYAQVKNYIVTVAANYGGGET
                     DRAQALTRLVPPAIQPVPLPSGFDVTVALSGQLPTSRLLYAVLPTLAPFATTDFSATP
                     RVTIEYVLSAIAAAQKDLINDDITLINGAFRQQVWKDTAGGFYSIWYANPVSIAAGNH
                     GFAAAIPWSSLAHEIGHNFTLNFPSGFIYGGRVDGNANAIYSETMAQIFQHAVIHEVV
                     NNSTAWGLSSEIAFELAQDARTTISVVRQNYENYLRTGKPFATWNNPSTPTDETLQTF
                     MTLAYLFLANVEQAGTGYRIPVKRLTKFLQRFNTQWQSSYAPSENTVQADTFRSTLMA
                     AAISYGVGRDLRLDFRTLGFPVDNAIYDSLNIGLENEITTKILPQFAFGGGWYSALYL
                     TNTSTNPVYFTVDFVGDNGERLIVPRLGSSSINASLGRRATAVVEAPNVGPLNQGFVE
                     LSLPSAVVGYGLFRQSIIGWADQEAVVPLSRMGTKASTLIWDERNLVTAVAIVNPGSQ
                     SNIVTIVGRDSEGGMVGTGILPMGPKTKVTALLRDLPGMGNIVGRSGSAEFVVDQGSI
                     AVLGLRFGGEAFTSIPTADR"
BASE COUNT         5174 a         7458 c         7468 g         5352 t
ORIGIN      
        1 tcggatccag cgggccggcc agctctttca gaccgtcgat cgtgaaccgg cggaagtcct
       61 tggggtactc atgaaaggga tgacaaaagg gcgttacgag gtgaacgtat ccacccggca
      121 ccaaaacgcg gcggatttcg ctcaggaccc gttcgggatt acggacgtgt tccaggaccg
      181 cgtcacattc cacacgctga aaagcatccg ccgggaatgg taactgctcc gcatccgcag
      241 ccacgtcgac gccgggaatt gcaagcagat cgatgttgat gtagccctga accgtacggc
      301 ccgcaccacc gatatacagg ttccaccggc caagggggaa atccgtgggc tcggctggat
      361 tcatcacgaa agggtctgga ggatgtaaag cgcgtagaag ccgatcactc cagcggtgtg
      421 aagctcgctt ccgataccgt aattcaaaat ccgtctcagg cattccgggc atcgatcagg
      481 agcggaaaga atttgccctc catctttttc ccggtgggaa tgggcggcac tcccggtaat
      541 ggctccgcgt cgctggcgga atgaacccca tcgcgaaaga cgttgatgac cgcggcgcgc
      601 cgcggactgc cggttcggtt ctcataggag ccgtgcacca tcaagggatg gtggaacgaa
      661 gcctctcccc gtttcaattg cacgggcaca ggatggaact gcgcctcctg ctccggcgag
      721 aggaccgttc gaatctcgtc catatcgccg gccagtccgg tgatcggtag cagcggccat
      781 cgatggctgc cgggcacgta atgtacgcat ccgttgtcga tcgtgctgtc gtccaagcca
      841 atccagcatg tcaagtgagc catgggctgc gttcgcgtcc agtaggagta gtcctggtgc
      901 caggcgacca ctcctccgtg atgggcgggc ttgcagaaaa gctggtcgtg ccagaacctt
      961 accgagtcgc caagcagttg catcgcggga acaagaaacg ccggattcca gagcaggtcg
     1021 tgcagccccg gacgaaatcg ccacccgccc agcgcgtgaa agagaatccg gctggagtct
     1081 tccgattcat tcgagtagta ctggttgaac aactcgattc cgggactcga cgggttcatc
     1141 gcctcgtcga tctcgggcag gattcggtcc gcttgctctt cggtgagtac ccgaatgccc
     1201 gccaggtatc catgctcgtg gaagaacgcc acctgttccc ttgtcaggcg gtactggtcc
     1261 cactccgcgg cggtttgcgg tgaacgaaag atgtccgtga tgagttcgtg ccggtcagct
     1321 aagtctgaga tcggcttgcc cttctctgaa tgcatgacag tcatttgcgt attctctctt
     1381 atcggaagcg gcaaacccag cgaggtgtca tcatagagac ttccctcccg aattgagaat
     1441 gaatatgacc ggtcgcgaac gcattgcccg gactatgagg catcacgagc ccgagagggt
     1501 tccggtgatg tgccagcttt ccataggcca catgaatctg aacggcggtt acaagccgca
     1561 cgagatctgg tacgacacgg aagccttcgc cgacgcttcc gtgaaactgg cgcggcgata
     1621 cggcttcgac ggcattctgg ttgttcttac gggccgcccg ccggattacc tcgacaatgt
     1681 tcaatccatc cgcgaagaca gcgacggcga gtggatcaca tggcgcaatg gagatcgtac
     1741 gtttctgccg tgggatgata tgccgcagca ctatccgtgc gatacctcgg ccccggtacg
     1801 ggccgttttc gaaacgttcg atcccgaacg cgacatcctg cggctggacc attatctggg
     1861 ctacgtctgg aacgtgctgt atcacatcca ggaggtgccg ggtaaacccg atgccgggcc
     1921 tctacgatcg gggtctgttc cggagtatct gttgcgcgcc ttcgacctgg tccggagccg
     1981 ggtgggagcc gaactgtccg tgcacggctc catctactca cccctgacac attatttcga
     2041 actgttcgga tatgaaaccg cgctcctcac ttttgtggac gacccgggaa gagcgcacgc
     2101 gattttggaa cggctgagcg acggagtgat tgcctggtcg ctggcgctgc ttgcacgggg
     2161 cgccgacgca ctcgaccact cctccgcttt cgtcgccgct cctttcctgt cgcggaagct
     2221 atatgaggag tttgtcgttc cctatgaagc gcgagtcaac gaggcgatcc gcgcggcagg
     2281 cggcatcgtc tacacccata cgtgcgggcg catcgccgac cggctcgacc tgatcgaggc
     2341 caccaggacc atgggaatcg atacgctcga cccgccgcct ttgggaaatt gcgatttggc
     2401 gcaagccaag cgcgccgttg gcgaacggct gttcctgaaa ggcaacatga actccgtggc
     2461 gttgctcgac tataaaacac ccgagcaagt gaggctcgag gccgttcgtg ccctcgaagc
     2521 gggcaagccc ggagcgggct atattctgag caccgcgtgt tcggtagcac cccgcgtcga
     2581 accctggaaa ctggagatgc tggcgccgat tgcgaatgag ctgggacagt atgagtgaaa
     2641 cggcaggcac acgacttcca tgcgcttctg gtacctgttc tgggtagtca cttttgccat
     2701 ttccgcggtg gcgttcgcgg cgattgcggc tgtggtggcg gttcgcggtg tgggcgatct
     2761 gcgccagatg ctggcggcgc ttaaggacgg agcggacaag tcttgaactt cacccccctc
     2821 gattgggtca tcgttctggt ttaccttgcc gtgaccatgg ctgcgggtct cgccggcaag
     2881 aagtacgtcc tgggtgtgtc cgactttctg gtagcggggc gaaagatcgg gccgttcgtc
     2941 ggaatcgcga cgctcgcggc aaccgaaatc ggaacgatca ccttcatgta ctacgccgag
     3001 ctcggctaca agaccggttt tgcgtccttc atcaacggcc tgatcgccgg ctgcgtcatg
     3061 atctttctcg gccgtaccgg cttcatggtt cacaagctgc gcgagcttcg catcatgacc
     3121 gtccccgagt tcttcgagtt gaagtacagc cgccggttgc gggtgctcac gggcgtgctt
     3181 gtctcgatcg gcggaatcct caacatggga gtcttcctgc gtgtcgaggg aacctttctc
     3241 accatcattg cgggtcttcc gctcgattat ctgcaaatca cgatgacggt catactcctg
     3301 ctcgagatcg tatatacagt gctgggaggc atggtttcca tcgtcatcac ggacttcatt
     3361 cagttcgtca tgctgagcat tggcacggtg ctggtgacgg tgtatgcgat gacggttgcc
     3421 ggatggggca acatgttcga cgcggtggcc gggacgatgg gtgagcccgg cttcaacccg
     3481 ctgacgaatc cggagtacgg ctgggcgtac attctttttc agatcctgtt gtggatgtcc
     3541 gttgatacgt gctggcagac gacggcgatg cgcacgttct ccacccgcga cccggaaaca
     3601 agcaagaagg tttttttctg gacgggcttc atctatctcg gccgcggcat gatgccgatg
     3661 ctgtggggca ttgcggcgct ggcgcttctc ggcccgcaac aggattcgct gcacgcgatg
     3721 ccgcagatga tcgcgcgcat tctgccggcg ggaatcctgg ggctggtagt ggcagggatg
     3781 ctggcggcta cgatgtccgt gaacagttcg tatctgctcg gttggagttc cgtcattgcg
     3841 caggatgtga ttgcgccgtt gcgcaagaca ccgatgagcc cccggcgcca gatcgtcgtc
     3901 aaccggataa cgaacctcct ggtcagcctg tttgtacttt tctggggagt ctggtacaca
     3961 ctgcccggcc ccgtctggtt ttatctgaac atcaccggca ccattttcct cgccggagcc
     4021 ttttccgcgt tcatcggcgg tcttttctgg aaaaaggcga atgtggtggg tggatacgcc
     4081 gcgatgctcg ccggcgcttc cggatcgatc gcgtttttct ttttcaaagt gccggtgagc
     4141 tacgccggac tcggctcgtt tgcgctggcg gccgtgggga tggtgggcgg ctccctcatt
     4201 ggcgacagga tgcgaaagcg ctctgcggct tgatgctgtt actcccggca gcttgcagcg
     4261 gggttaagcg ctcttcttga tgtcttccag catcgcttcc cggagcacct gattgagccg
     4321 tgtccgatag cctctgccct gccggcgaag ccacgccacg acatcggcgt ccagccggac
     4381 ggtgagctgc tttgaccggg cggtagaagg gattgcccaa acgctttcgg caactggcac
     4441 acgatttatg tacgcatgaa tcgctgggcc aagggcggcg tgctggacag ggtgttcgag
     4501 caactgcaac gtgaacaact ggtgcgcatc aagatcgaag cgatcagcct ggattcaact
     4561 tcggtgaaag tccatccgga tgcaacgggc gttaaaaaaa cgggccacaa gccatcggaa
     4621 aatcgcgcgg cggatggacc accaagattc atctggttgc cgcgaatgct cgaacggctc
     4681 taaccttcgc gttgtcgccc ggccaagcgc acgatgcggc tccaggccgg gagttgctgc
     4741 ggaatctgcc cccgttgccg gaagacttgc cggtcctgat ggatggggcc taccaggaca
     4801 accagacccg gcaactggtc ctgggtctgg gttgggttcc ggtggttcca cccgcggcga
     4861 atcgcatcga accgtgggag tacgatcgtg cgctgtacaa gaaacgcaac gaaatcgagc
     4921 gtttattccg caggcttaag ggcttccgca ggatcttctc ccgcttcgag aaactcgatg
     4981 tgctcttcct tggcttcctt cactttgcgc tcatcgccga tgccttgcga tagtgtgaac
     5041 acgccctagt ttgaaggcaa cagatcggct actctcaggc acacttctcg tgacggactc
     5101 attacggcgt agatgccgtt tttggtccca aaaacaagcg tcacacgttc agtttcaccg
     5161 tcgtccttga actcctcgac aaggaggccc gagccccacc gcagggatac ctcctcgatc
     5221 ctcgccgcct cgtcggtcgg aatgccgatg agcaatgagg gctgtgccac gtaacgtcgt
     5281 gccagggcgc gagcttccca ccaggacaat ccgaggctcc ggaatgctgt ttctgcgaac
     5341 tgcgacaagg gaaatcccgt agggagaaac agctgcaccg gcttggcttg cagaatctgg
     5401 atctcgcccg gataatccgc cacgaccagc ggtcccatgc tggcacgcat cgtcagctga
     5461 tcccactcgt ctggaactac gacgtccgat gcaccaatgc gcgcgagagc agatcgcaac
     5521 tcgcttgtgc gaatagtctg ccgcattgtc ataggcccga tcaccgatat ggccggcggg
     5581 cccggccagg agtcaaacac cggcaaacgg ggccggaaac cggcttgcgc ctctgcctcg
     5641 gagagtcccg ccacgacacg ttggccgccg gagcccgtga agtcggtgtc aaacggcgca
     5701 tcagacaggt ccacccgaac aacatcgatc cgattcagaa agagtcggaa ccacagctcc
     5761 tgcgccaacg ctcggccttg tggcaacgcg agaatcgcca aggccaatcc tgcaagcgcc
     5821 gaagccgcta tccacgcctt tctctgcggt tctggccgca gtttccggtt gagcgtcaca
     5881 cgcgcctgcg cgacggtggg agtccatgcg ggctcaacct tcaagagccg ctcagcgacc
     5941 cagcgactgt tatcgtttgt ttccatatcg tctctcgtac tccttccgga acgcggtttg
     6001 tgcacggctc aagagacttc cgacagaggt gaggttgatg tcggccgccg cagcgatttc
     6061 gcgataagtc agttcctcgg cccacagcag cagcacttgt gcgtgccgcg ggtcaatggc
     6121 cgcgagcgcc gcacgtacgt tcgtctgctc gacgtccgcc tcgtacaatt cctgaggcgt
     6181 tggaggtgat ccgcggagtg acgaaaacaa acgctcaaat cgttcccgtc gtgcttgctt
     6241 ccgccactca tcgatcgctt ctcgcactgc tgtgcggtat aaccagcctt cagcctgctc
     6301 gccttgtgct ctgggattgc gctgccattt gacgaacacc tcgacggcta actcttccgc
     6361 gcgggcctgg tcacggacga ctcggccaat gacgcgggca atgcgcccat aatgggcgtt
     6421 aaaaagcgtg tcgaggtcca tttgtcccgt cgtcccagct cttgacatcg ccatgctgct
     6481 tgcgttcaca gtacaagaac gtttgggtgg gacggtttgt gccagtgtca gccggttttt
     6541 gtcgttatgc agcggacgtc attcttaggc tggtgcgttc tgcggcagat gcacgccgtt
     6601 aagcgtcaga agcgcttagc gtacactttt ctccttttgg tgccgcgtgg cctgttatgg
     6661 cagtgcggct gttgttgggg catatacgat aggttggtgc gccgttttct gctccgcgcc
     6721 acacgccggc tactgaaaga gatcgatctc caccttgagc gggatggcga ttcggccggg
     6781 cgtgtcattg ctgcgggcaa tggccgggag accgggatcg aacggttttt gcgcgccgca
     6841 atactgccgg acgtggcccg ccaatatctc gaccaccacc ttccgagact aatccgcaca
     6901 tttactctgc ttccgcccaa cggcggctcg gcgctcgaac tcgggtcgta cgtctacggc
     6961 gcggctgtgt tggagcgcgt cctgggatac cgcgcggtac gtggcgccta ttatggcgcg
     7021 cagccaggat cggatcgaaa gactctcgaa atcgccggtc agcccgtctt cgaactcgac
     7081 atcgatttgt tcgacgcgga aacgcattca tacccgtatc cggacgggac gttcgacgct
     7141 gtgctttgct gcgaagtgat cgaacacttg cttcgagatc cgatgtatct tctattcgag
     7201 tgccggcggg ttttgaaaga gggcggtctg cttctgctca caacgccgaa cgccgccagc
     7261 ttgacgagtg tggctcggac cctgcatgga aggaaaagcc cgcaactgtt ttctgcctat
     7321 ccggcgcccg ggaatgcgga tacaccgcac gtccgcgaat acacggtttc cgaattggcc
     7381 gctgtcgtga cagcaggcgg cttccacacc gaagtgattt ttactgaggt gctggagggc
     7441 tttgagaaca cctcgtgggt gaagcctttg ctcgaacggg aaggcttcga taccgcaatg
     7501 cgcggcgaac aagcctattg cctcgcgcgg aagcagtcta cattgccttg cgaacggtac
     7561 ccgcgttggc tctatgccat ttagcagggt tacgccactg agtcgcatcg gcagagcggg
     7621 aagtgctcac gcggccgcac cgcaacgaga agagcgccgt gttcgctccg attcccagcg
     7681 cgagggaaac cagggcggcc aacgtgaaac ccggtgaacg ccataggccg cgcaatccgt
     7741 aacggagatc ctgaagcgtt ggcagccggc aaggcagacc cttcttttac tcccggcgga
     7801 gggtctgcat cgggtccacc gacgcagcac gcaggcccgg cacagccgct gcggcaagcc
     7861 cgatgagcag catgaaaacg gcacttgcgg cgaatgccgc cggatcggcc ggtttgacgc
     7921 cgaataacaa ccctgctgcc aggcgcgcgc cgatgagcgc aagtggaatc ccgatcgcag
     7981 cgcccgcgaa gatcaatatc agcccgtcgc ggagcaccag ccaaagcatt cttcccggcg
     8041 cagccccgag tgctacgcga atgccgattt ccgcggtgcg ttgcgcaacc aggtaggaca
     8101 taactccgta cagccctaca ccggcgagaa cgagcgacag gattgcgaac agcacgctta
     8161 cagaagcgat aagccggtcc agaaaaatag agtcttcaaa ccgctggcgg acggtacgca
     8221 cgccgctgag agtgacggcc cgatccagcg cggccagttc acgccgaaga agcggaacca
     8281 gggtcccggt gctgacaccc gcacggacaa gaagaatggg atcggaagta ctctccatct
     8341 gggtgaccgg caaataaacg gtcggtacag gatgttcccg cacaccttgg tgcaacatgt
     8401 ttcgaacctg ccccacaatg tagatgggaa ttccaccttg cggctcctta tcatcgaagc
     8461 tgagggtgtg tcccacggga tcggctccat tcagatattc gcgggagaat gcctcattta
     8521 caatcgcgac tttccgcatc tgctccgcac cgctgtcctg aaagtcacgc ccgcggaccg
     8581 ggaggctacc gattgtctcg aagtacctgg ccgccacgga ttgcaattcc acagaagccg
     8641 gttccacggc tgtacgctcc gaaccgggaa ccctgattcc ggcgctccag gttccgccct
     8701 ggtaaggccc tggaaacccg tagctgacgg cagtgacacc gggaatcgca cggatacgtt
     8761 gcaccaggcg ttcgcggatg gatgcaacct ggcttgattt gtacgagcgc ggagagtcga
     8821 actgaaaagt cagaacatca tagtggtgga atccggtgtc ggtagtattc aatgcgaaga
     8881 ggctgcggct gaataaggta gcgcccgata caagtacgat gcagagcgca acctgagtaa
     8941 caacaagggt ttttcgcacg cggtggcggc accctcgccc gcttccggca gatccttctt
     9001 tcagcacagc acccggtgga acgttcgtgg aaactaaagc gggcagaaga ccgaacagga
     9061 acatcgcagc cagggagaca gtcacggtaa ataagataac gttcgcatcc ggcttaacat
     9121 cgagagtcag gttggcatcg tccaccggca ggaactgcac gatgttgcgg ctgccccata
     9181 tgcccagcgc gattccagcc ggtattccca cggcagcgag aagggacgtc tccgtaagcc
     9241 attgacggac caggcgcaag cggctcgcgc caagcgacag gcgcagcgcg atctcgcggc
     9301 ggcgcgcagt tccccgcgcg agcagcagat tagcgacatt cgagcaggcg attaaaagaa
     9361 tcagcgtcac caaggccatg agcagcagca gcggtttttg gaactggtcg cgtaggaagg
     9421 aaacaccggt gcctccggcc cgaagttcga gccgctgccc catccgcttg cgctgccagg
     9481 gcgtattggg atcgttcgca taattgctat tcaaaaaatt gcgaaccagc acattcactt
     9541 tcgcctgtgc ctcttcgccc tggacgccgg gctgcagccg cgccagcagc cagagccaat
     9601 gcatacccgc atcctgaaaa cgcgcccggt gcatggtgat ggggacccat acatccgcct
     9661 gtccttccac ctgtacgccc cgaaaaccct tccgggctac gccaatgatc gtaagaggct
     9721 ggtcatcgat gacgatttgt ttgccgggga cgttcgggtc gccataaaag cgggcttgcc
     9781 agaaatcgta attgagaacc gcatacggct gcgcctgtgg actgctttcc tctccggcaa
     9841 agagacgtcc gagcgccggc tgcacaccga gcacttcaaa gtagttcgcg gaaacgaatt
     9901 cgcgaaacag aaaatccggt tgttctgtgc ttccggccgt ggtgtagcgc acgcgccatg
     9961 gtgcgctccg cgccaatacg ccttcaaaca ggtccgtctg tttgagcagt tcctgataga
    10021 ggggatacga cgtaaaccct cgcccccaac cgcccacccg gtacaaggac acgatttgtt
    10081 ccggctcgcg gaccggcagc attcgaagca tcagcgtgtt gaacaacgag aagatcgccg
    10141 tgttcgctcc gattcccagc gcgagggaaa tcatggcggc caatgtgaaa cccggtgaac
    10201 gccataggcc gcgcaatccg taacggagat cttgaagcgt cgtctcgatc agcgggacgc
    10261 ctcgctgctc gcggtaagct tccttcatcc gggcaacgcc gccaaaatca cgccgggccg
    10321 cggcgcgagc ttcgacctgc ggcatcccct tgcgctggta ttcttcggca agcgtatcga
    10381 ggtgaaaccg gacctcatct tcgagatcgc gttctgcgca tgttccggcc aacagcgccg
    10441 acaaccgccg gaaggcggat cgcatcagcc ctcctctccg gttcgcagaa accggctcat
    10501 gagggatacg attcgcgtcc agcccgcctg ttcttccgcg atctggcgcc gtccggctgc
    10561 cgtgaggctg tagaaacggg cccggcgatt gttatccgac gtaccccact tggaccggat
    10621 ccaacctcgc tgctcgagcc gcagcaaagc aggatatagc gtgccctggt tcagggacag
    10681 cgtcccttcc gagatctgtt caatccgttt ggcgagaccg aggccgtgct gcggccccat
    10741 cgtctcgagt gtccgcaaca ccatcagatc cagggttcct tgcagaacct cgggctggct
    10801 ttcttgttta gacatccaaa aggagtatgc ttccgcttgt ttagaatgtc aagaggaatg
    10861 tgccgcacgg tgttgaggtg gctatctgga aaatggattt tcgatccgca cacccgcgat
    10921 ggtctggcct gcattcaggt cttccgacag aattcgaccg gcaccggcct tccacgcggc
    10981 tgcaatgatc agcgcgtccc agaagccgat acacgcctca tcctcgatgc gaaaagcggc
    11041 agaaatttca gccagggtgg tatccacgca ccagacagca taactgtcca caacagcgcg
    11101 ggcagaggat ttcggcaggg gtgaggcgat ctttctcgtc acattgacgt agaactcgtg
    11161 gagcacctgt gtactgagga cgccggtgcg ccgctcccac aattcccgca gaatgccctt
    11221 ggcgatttcg tgcttcgctt tggcatcggc atcgtgggca taaatgagaa tatttgtgtc
    11281 cacaaatgtt ttagcgttca tgcaactcgt cccgactggc aggagggaac ccgcctaaat
    11341 gaaagccttc ttccagcaag gccaatgctg atcgttcaga gcgttcgtag gcttcgtccg
    11401 cgccaacaag aatttcaatc tgttcggcca ggaggccgct gatcgaagtc gaacgccgtg
    11461 ccgcaagaat tttagctttc cgtacgatgt ccgggctaat gctgatcgtg acattttgct
    11521 ttttgatatt ttcccgggcc atgtcttcct ttttagccac gtataatacg tgctacacgt
    11581 attatacgtg gaaaggtggc gcgaaagcag cgatcctttt gtagcgctgc ccgtaatctt
    11641 caggtgaggg acgcagacga gagccgaaat gacggacgcc gccatactcg ccgcctggta
    11701 cggccacaaa gacaccatat accggtttgc gtggcgtatg acgggatcgg cgtccatcgc
    11761 ggaagacgtg ctacaagagg tgttccttgc tctcctccgc gtgccagacc acttcgacgt
    11821 ccgccgtgct ccgctgcggg cattcctgct ggccgtcgcg cgcaaccagg tgcgcaagcg
    11881 atgggaaaaa gaacgccggt gggacaccct ggacgatgac atgtttgaag cggcgccgat
    11941 gaatctgctc gcggcagaga ttgcggattc ggtagcacgt gcggttcagt ccctgccacc
    12001 gcgacagcgg gagacactcg tgcttttcga gtacgagggg ctctcgctcg aagaagttgc
    12061 gcgggccgtg gatgcggagg ttggagccgt caagtcccgt ctgaaccggg cccgggaaaa
    12121 tctgcgcctt atactggcgc cgctgagagg agcttatgga gcctttaaat gatcacgagt
    12181 tgcaaaccct gctaaaggcc tgggatgcgc cggaggcacc agaacatctg cagcctcctc
    12241 tgccccggcg ccggtggtac gccgtgctat ggggggcgtc cgtacacatc ccggtgccgg
    12301 tgctcgcatt gattctgatg gccatcatcg ccctgcaggt atttccgcgg cgcgaacgca
    12361 cacctattcc gccggacgcc ctgcgcgaga tccggctttc ggacttcgag cctgttcccg
    12421 aagccaaacc tgtggtggtg aggaggacgc actatgagaa tcattagtct gctgctgctg
    12481 agcgccacac tgagtccggc gtatcaggtg atctcgagcg gatcgggttc gaaggacggg
    12541 gtggggttcc gttacgaaac ccgcgtggaa ccacgcatcg aagggcagaa ggtgattgga
    12601 ttttcaggcg gcggaattta catcagagat acgtttcatc gttacttgcg agacgatgcg
    12661 acgcgccgct atgtgggcta cgacctcatc ctcaacaagc ggaccgacgg aatgctggtc
    12721 gtgactttcg gacaactgag cgtgtccggg gagaaacttg gactcaaagg cgaatggccg
    12781 caggttcctc tacgcgtgaa ctcgacgccg cagaccatca agccgggcga cgagatcgcc
    12841 attgacctgt ttacgaatcc ggccactggc cagaagattc tggatcacct cgttttcggt
    12901 gaggctccgg aggtggtgcg cctgcctgag ggctctccgc gcgatttcac aatggatgac
    12961 gtgccgctga ccctgacgag tccgaaaatc ctgctgaacg ggaagcaact ggaatcaatg
    13021 gaggccgcta tcagcggcga ggcggtgtac ttctacctgc caggccacgg ccgctacgcg
    13081 atgtcgcttg cgccgaatct ggaactcggg ttccgcaagt tggacgaggt ccgtgggtca
    13141 cggatcagct tccgcaacgg gaacgatacc tggactatcg actgtacgca tcccgtcgca
    13201 ccgggcggcg gagtattcaa cctgtacgtc taccccgacc cggtttggca accatcgagg
    13261 ggcgcggaca cctaccagct tggggccagt tccgcacgcg cgatgatccg aaaatagccg
    13321 ttcatttcgt cagggcgttt cgtttatagc cccatcagaa tccgcagccg ggaccggacg
    13381 cctgctcgcg tccttgagta ggatcgagat taaaggacca gatctcaccc cccgaaggct
    13441 tgggaagact cactcccgct tcagtccgtc gccactcgtc cggtcccata gatcccgttc
    13501 ctgttgctcc ccggtccatg cctccggatt gttccgaaga gcggcgaacg cagcattggc
    13561 ttctttgatg aattttctcc ggcggtaact gtttatggct tgcttgagga cggtctgaac
    13621 tggctggcgc tgccttcggg cgaggtcccg cagtacggcg agagcctgct catcgatgcg
    13681 gatgctggta ctcggcatgg gctccaaagg ctacaacaat tttagtccgg caggtagaag
    13741 ctggaacttt ccgttggccg ggcatgcagg tagcggccgg ttgtcgaaac cgaggcatgg
    13801 ccgagggtcg cctgcacgag gtggatcggg gcgcggcggt ccagcgcgtg gctggcgtgg
    13861 gcgtggcgca tccagtgcgg tgagaccttt tgttcgagac cggctttctt tgcagcggcg
    13921 taaacaatgc ggcgcacttg ggaggcgtcg agatgaccgc cgcccttccg gctgcggaag
    13981 acgggatcga ccggaccggc ctcgccacgg atcgccagca gttgcgacca gaacttcggc
    14041 ttcagcagga tcgtacgtgt cttgccgcgc ttgccgaaaa cggtgatctg ccctccttca
    14101 gtccgcggca gggcgtcgca ccacttcagt ccgcaaagtt cgctgacgcg gacgccggag
    14161 acatagagga gcttcagcag tacccgatca cggccctccg gcgccgctcc aatgagtttg
    14221 gcaacctcgc tttcctcgag aatgcgttgg gcgagcgagt cgcggttcgg ccggagtttc
    14281 acggccgctc cgacattaaa aggaaggtag ccggtctcct gcccgaagga caggagcgat
    14341 ttaacggcgg tgagggcccg gttctggctg gcgggtttga gacttccctg ctccaaggcg
    14401 gccgcatacg cctgaacatc ggcgagcgtc acccacccga gcggtttggc aacgaacatc
    14461 aggaaccgct ccacatccgc ggcataggcg cgccgggtat gcgagctgac cttcagcgac
    14521 agccagagtt cgaccaggct ggcatcgttc gagccatccg gcgagggctg gacggcaaac
    14581 gccggcgccg atgtgagttc gttcactcct caagtagtat acacggtata tctcgttatc
    14641 gtgcattaaa taatatatgg gtatctggta atatggcgat atggcaagca gaaaatttac
    14701 ctttacctta ccgcaggatt tagccactga gtttctgcat cgcgtgccgg cgagcagccg
    14761 atcgcaatac gcagcggcgg cgattgcggc caagcttcgg gaacgtgagc aacaactcgc
    14821 cctggcatgc gaagtggcga acaactctgc tgatgtccgg gagattgaaa cttcttttga
    14881 tgcgctggcg gatgaagccg acaccgtgca ggagccatgg tagagccccg gcgcggtgaa
    14941 gtctggtggg ttcgtctgga tctcaccttg ggctcagaga ttgctaagac ccggccgtgc
    15001 gtgatccttt ctggtaacat tttcaataag ctacgccgga ccgtggtggt aattccgctg
    15061 tccagctcgc cgcaatccag cgcccccttg ctagtgcctg tgcggtgtga cggccgggac
    15121 gtagtagcgg taacggatca gatacgggcc gtctccaaac aacgactcga tcagcggctg
    15181 ggggagttgt catccgagga cctgaaggcg gttgaagggg gcgtgcggga agttctggaa
    15241 ttatagttcg caggatattg cacgatcgtg caatatcata gagaagccgc tatactttct
    15301 acaggtttcc ctccatgccg gacaacccct cgtatattca taaacttgag ggcatcctgg
    15361 ccgaggcgcg ctcgcctaaa ccaattccct tcttccgccg ccgagacatt gaggccctct
    15421 tcgggttaaa aaaacgccaa gccgtcaacc tcatgcaccg gatcggagcg attcgtgtca
    15481 gccgcgagct tgctgtcgag caatgcgatt taatccggtg gctagaacag atgctctcgg
    15541 acccttccgt tgcaatcgag cagcgccggc acgagcgcgt cattagccgg attgtcgaac
    15601 tcaaagccga aaccgcggcc cgggcggtcc gaatcgtcct acccgacccg gcgccttccg
    15661 ttgatttccc cgaaggtgtt tcgctccaac ctggctcgct gaccgtctcc ttcgagaacg
    15721 aacagcaact gctcgagcgg ctctttctgc tggcgcgtgt gctggctaca cagcctcaga
    15781 tactgaacaa catataaaac aggtataccc gtatcaggta tgccttatcg gattaccagt
    15841 cagtggatcc acaaagacaa ccccccggtt cttgatgtaa tgggtcagtc ccgctgactg
    15901 gcggcgtttc agaatcgcat cggcgctgta taccaggtag gttgttccgg cactgggcgt
    15961 atggcgctcc gtttcttcaa tagccagctt atcccgcagg ccttgcagat tcgccttgca
    16021 gttgttggcc gtcaggccgc aaacggcgct gagagtgcga tagccgatcg taacgatgcg
    16081 gctgtgcggg tcgcggggga cgccgtgatc ccagagtgcc tgataaaccg cctgttcgcc
    16141 gagagaatgg ccatcctgcg cgcggaaggc ttggcggata cggcgcagga gagcgatttg
    16201 ggtgacggca acattgtgtg gggttacacc tgaagcaggt atacctcttt caggtatcgc
    16261 aatatcgatc ccttctttat caggtacaga cgattcaggt atttttaaaa caggtatacc
    16321 tgcttcaggt gccatttgtt ctgtggaaga cgacgtttct tccggcacag gaccaccttc
    16381 ttcagcaata cctgaagcag gtgtacctga ttccgcaggg cggggaactt ccaccacctg
    16441 cggaccccgc agaagaaagc tgaccgcact ccgcatatcg gcatccgtcg gccgcagaaa
    16501 ctcggttacg tcgaaatctt cagacgtttg ccgtttgcgt ttggccatcg gatgcctcaa
    16561 tcatcgcggt gatttgtgca aaggcggcaa gatagtcctg tgccgctttg gagtttggat
    16621 cgtagtcgag aagggccttg cgcgcccgga acgccttgtt gacggtctga tcggtgcgga
    16681 tggcgggaag gattggaacc tcgaacatct cggacatctt ctgcaggctt tgctggacca
    16741 gagcggtaac cgacagccgc tggtccacct ggcagggcat caggccgatc gcgcggatat
    16801 tcgccttaat cagatcgttc agaagccgca tcatgtcgta agcggcattg gcgccgttga
    16861 ggctcagcag gtccatgtcg accggaataa cgacattacg ggcatagacc atggcgcagg
    16921 attgcagcag gttaatcgat ggggcgacgt caatgactac ggcgtcgtag cgggactcat
    16981 agggctccat catgatcttc aatgccagct cgcggaccac ggcgcccgcc atcgccgatt
    17041 cggcctgaat ggtgtccttg tttccacaca gaatgtcgag cttatcggtg actttcacgg
    17101 tgcactcgtc caggtggaac ttgtggatca gaaactgatt gaagaagtta cggggtttca
    17161 ggttcagcat cacgctgatc gacccctggc tgtcggtatc gatcagcagt gtcctatgcc
    17221 ccttgccggc caggtaccag gcatagttca cggcacaggt ggtcttgccc acgccgcctc
    17281 gctgattagc aaccactacc cggatcatcg gacctccggt acggcttgcc tgcgaatccg
    17341 ctccacatcc tcaacagtcc acaaacgata gttgttgctg gggtttcgat acggttcggg
    17401 gattttcgac tctttcagcc agcggtacag ggtggccagg gatatgccta gagactccgc
    17461 aacctgtgct gtcttcagat aatgccggcg catgcacaaa ttgtatgtgt gactagggtt
    17521 atggtgcaac tcacaaaagt cttgtcaaga aggatgcggt ccgtcaggcg gccgaactgg
    17581 actggcgtac ttcggtctgt ttccagagtt ttcggctggg aatgaacaaa acagagatgc
    17641 cgcacagggc tgccgcgatc agcacgacga acgtccagca gggagaagta atacgcttga
    17701 tcaacgagcc ttccggaatc agggggttta gtagagcgac aagataaaac cccgcctgag
    17761 cggagagcgc tgcccacttc cataccggcg gcaggccaaa gctggaagcc aaaaggagca
    17821 gcaacgcgaa cggcagcagc aggcggccca ccttatggga tatgaaatga aaccacattc
    17881 gattggaagg gccaagcagg gcggggaagg agcgaatggt ttgatacact cccgcctgtg
    17941 tgcggacctt ccgatgaaac tcggccttca gcgacgtggg atagtcgaac gccttggcgg
    18001 attcgtccag gatgatccgg tagccacgga aaaacgcgcc cagcgggagt tgcacgtcgt
    18061 cgagcagcgt atccggagag agcggagcgg ccagttcacg gcgcatggcg tagatacacc
    18121 cgctggcgcc gatcaccgaa tccacacggc tcaagtgctt ccggatccat ttttcgtatc
    18181 tccagtaaag ccctacgttc agctcaccaa gggtttcccc tttgcggata atgagctctc
    18241 cgctggcgac tcccacctgc gggtcggaaa aacagctcac cagatgagcc aggctggaag
    18301 gctcgagctg ctggcggacg tcggtgaaaa acaggatgtc acccgtggca tgctgcacgg
    18361 ccgtgttcaa cgcacaagct tttccgcttc ttggaatgcg gtaaaagcgg atacgggcag
    18421 attcgaactg cttgacgatt tcgtcggtcc tatcggtaga accatctgaa accacaatga
    18481 tttccaccaa ctcgcggggg tatttgagcg cctggatgga ttcgagcttc tgctgaatcc
    18541 aaagctctcc gttgtgtacg ggcagcagca cggatactga aaaatggtgc gagggtttct
    18601 tccggatgga ggcacccctg cggctaagaa gagccagcag cacgggatac cccatcaaca
    18661 catagacaat aaagatcgca ctcagtagga atgtgccgat tgcgaggtca ttcatgagtg
    18721 gtaggtaata ggggggtaga ccagcctgga taatgccggt ttggccggat tggataggtt
    18781 caattgtcgg cgtcctaacg tttgcatggc ctccgagccg gtgggcgccc tcggcgttcc
    18841 tcttgttgta tacccagcta cttagacgcc gggcagggaa ctaccttaca agattaacac
    18901 ttttttgcca agcatcccac gggaattaac ctggagtatg cccggttgcg gcgccacatg
    18961 gattcagttc ttttgacagc tttttcgaat ggcttcagcg atttggccgg caacttccgc
    19021 gttgctcata agtgcccggt gcggcagcca gaacgcgtct tcgattcggg cttccgcatt
    19081 ggggcagggc tcaacacggc atccgccggc gcggtacagc gggttcccgt aaagcgggtg
    19141 cgggtaaaaa ggagtacacg gcacaccggc attggcgagc gaccggcaga aatcgtccct
    19201 ggaaacgccg agctttttct ggtcaacgcg gccgaggaac aggtaccagg aattgctgct
    19261 gacctccgga gcgatctcct gaaactgaag tccctttaca tcggcgagat cggatcggag
    19321 gaattgcgca ttccgtgtac gcaacgagat ctgttccggc agcctttcaa gctgtgcaag
    19381 cagaacggct gcctgaaagg ctgtgatgcg gtagttggtt cccagggtgt aatgatggaa
    19441 ccagccctgg tcctgacgcc ttccctggtt ggcaaaggac cgcacctgtt catcgaaacc
    19501 ggcatcgtta gaaagcagga tgccaccttc gcccgccgtc atcaccttcg agttttgaaa
    19561 gctgaaggat cccgccagac cgaaactgcc cgcacggcgg cctctccatt ccgaaccgtg
    19621 ggcatgggcg gcatcctcaa tcagcgccag tccatgcccg gcggccaatc gcggcaactg
    19681 atccatgtcg gccagaggtc cgccgaagtg gacggggatg atggcgcgcg ttttcgatgt
    19741 gatggcgcga gcggcgcact cggggtccat ttgaaagctc aggggaccga tatcgacgaa
    19801 aaccggaatg ccacccaccc tcgacacggc ggtagccgtc gaaatgaaag agatggcggg
    19861 cacaatgacc tcgtcgcctt ccttaactcc ggcagcctgc agggccatct cgagcgtgac
    19921 cgttccattc accgcggtta cgccatactt gcagtgctgg aattcggcga atgtgcgctc
    19981 gaagcgttct accacttcat gaaaaccgcc ccattggcgg ctgcacagca cttccttcag
    20041 agactccagt tcacgatccg tagcaatagg ccattcagga ataagcatgg ggcaccgggc
    20101 aggattggca aataggtggc aatgaagtaa ccatgttagc ccagttgctc cgttgtgttc
    20161 gcttaccccg ttctcgtgag agacttaggg tttgactcct gcgagcgccg ctcgcaagaa
    20221 ttcaaaggag agcctgaaag gccggctgca cattttaagc tttcaacccg gagggacatt
    20281 gaggtataga ctcgccgctc tgatttccat tctttgggcc gtctccgccg tggcacaggt
    20341 tatgccctcc ggcggtccat ctatcttgag ccgccagccc agcctgcgca ccggaccggc
    20401 agatgcattg ttcaccatca gaccttccgc cagcattgta ggcagatacg atcacggtct
    20461 tttcctaccc acgacggatg ccgagggcag gttgtcgggg caggacagct acggtatcgc
    20521 cgccgtcctt ggattggacg ggtttcatca aacgagaaac agcgttgtca gcctcgatta
    20581 ccggggacag ttccggcact acacggcgaa cgattatctg gacgggagcg accacgcgct
    20641 atcgctcggc tatgaacgcc ggctgaccaa ccggacgctc gttgccggaa cggcattcgg
    20701 cggtctttac tcccggtctt tcggggtgcc cggattgggg agaatgagcc cccccatcgt
    20761 cgatatcggc cccgagatta ttccgaccaa cgagattttc gatagccgcg tttattatgc
    20821 gggcgccggt gcgaatatcg tccatcaaaa atccatgagg ctatcgttcg gcgctggtgc
    20881 aaacgggttt atggtacacc gacggtcccg gtctctggcg ggcgtaatcg gtacttccgc
    20941 ccagggcaac gtcgcctacc ggttgtccag aagacacacc gtcggcgccg attatacctt
    21001 tacgaaattc gacttcacca ggggctttgg catatccgac atgcacatcg tgtcgttgaa
    21061 ttacgcagcc cagctcacgc gccgctggac cttttccgcc cgggcgggca tcttccggtt
    21121 cgagaatctc cgggttgtta ccgtgcaact cgatccggtg atcgcggaga ttctggggcg
    21181 caggacagga ttggaaaccc agcaccaggt gaactatggg ccgattgtaa gggccgtgct
    21241 gacgcgcgag ttccagcgcg ggagcttcaa tgccagctat agcagaatcg tcagcccggg
    21301 caacggaatt tacctgagct ccatcgcgga gtccgcacgc gtgggctaca actaccgtgg
    21361 ctcgcgccgg tggaattccg gcgtggctgc cggagtgact caatacggcg ccctgaccca
    21421 gactatcggg aaatatcaca acctttacgg cagcgcccag acatcctacc ggctgaaacg
    21481 gtttctgcac ctgacaggtt ccttcatcgt caggagccat gggattggcg agggttcgga
    21541 tttcaaacgg acatcctact cggcatccat cggcctggcg tttgctccag gcgagatccc
    21601 tctatcgctg aattaaacac cgcgccactt ctgtttgcgt atcccgctaa ccgctcccaa
    21661 agatggtcgc aatcttccgg gccggggcta tggttgctcc ctgcggtccc agtctggatt
    21721 tcatccgtct cagataaacg cccaattgag actggttccg atgcagagag tgggcgcact
    21781 gccggaagat ctggccggcg cggttgttga cgtcacgaga gccgcgccac catcggtttc
    21841 cgctcttgtc gttatccggg cacaaggcga gccaggaaac aaattggccg accgtgggga
    21901 atcggcttag atctgggccc aactccgtca gcagcgacag gcctgactgc tccagcccag
    21961 gaatttgtaa gacgtctacg ccaaaccgcc gatacagttc ggtgcgcatt gcaaagccgc
    22021 cgccttgcct taccccagcg ttccggctgt tgcgggtacg acgcttcttg ccatctggcg
    22081 gcaacggttt ctcctccggg tctactttcg gctcgatctc gttgagcagt tgcgcgattt
    22141 ccatatcgca ctgctcgatc tgtcgctgat agtggcccta cgcttcacgc gattgccgta
    22201 gcgtgaacag atgctcgcgg cgccagttgc ccacccgtga tttgcggatc gtctcggtat
    22261 ccgcctgaat ccggtaatgc cgcaactcgg ccagtttcgc cggatcgcgc tcaccgtcca
    22321 ggatggcgtc cactatggcc agttccgcca acccggcgat gtcgttgatg acacgatgaa
    22381 tctgtacgtt catctgcgtt agcgctttct gcatgtgctg cacatgctgc gccgccatta
    22441 tcactaattc gccccggtgc cgcatgatgg cgcgcacggc acagatttca tcgtccggcc
    22501 ggaaggcgcg gcgcaacagc ccaccgaatg caggtactga atccactggc agtcgtgcca
    22561 gtcggtgcgg cgtcccggta cgttcttcat atgccgtgca ttcaccaggc atggctgcag
    22621 ccctcgccgg tccaggattt cgtatagcgg cacccagtac acgccggtcg attccatcgc
    22681 gaccgtacga atcctgcatg cctccagcca gtcggccagc gcgttcagat cttcggtaaa
    22741 ggttgcgaaa acccgtaccg ggttctgatc gcggcccgga ggaaccgctg cgtaaatctc
    22801 cctcgcgcca atatcgactc cggcggcact gccttcgatc atcggcctgt ccgtcaggca
    22861 catccctttt cttcttcttt tcctcggttc gttgtgtttg ccatcgctga ctccgccttc
    22921 gcgctgtcag cggccggatc gcgttcatac atactcttct aaaggaaatc aaccccgcat
    22981 cgctgccggg ccgttcatca ctgtgctgcg cacctgatcc ggggccaggc tccctctggg
    23041 gctcttacac accactgcgc catcggacct gcttcgcgct gacatcgccc cttaagggtc
    23101 tactatcgcc ttggttcctt cacactcctg cctcgccgga ggcgatgacc ggccggagac
    23161 atcattgata ctttagttag accgggtaca tcgctgacag tttgctctgc aaagaagtgc
    23221 tcgtcaatcc tccgattatc taccctccgg cggcagctac cgctagtgag accgagccag
    23281 ccgtagaatg tgtcggtgga tcaactcagc ggcgcgtcca caatctcggc tctatcagcg
    23341 cctgggggag cgacccgcca gccgaggtgc ttctccagtt cccgcaacag tgtcgccatg
    23401 ggtagagacc gacctatctg tctgccgtcg gtatcgaggt aaaggcttcg ccgccgaaac
    23461 ggagtccgag aacagcgatg ctgccttgat caacgacaaa ttccgcagag ccgctccggc
    23521 caacgatgtt gcccatgcca ggaaggtctc ggagaagagc ggtgaccttg gtcttgggtc
    23581 ccatgggtaa aatcccagtt ccgaccattc caccttcgga gtcacggcca acgatcgtga
    23641 caatgttgct ttgagatccg ggattgacaa tcgcaactgc agtgacaaga ttcctctcat
    23701 cccagataag tgtgcttgcc ttcgtcccca tccttgagag tggtacgacc gcttcctgat
    23761 ctgcccaccc tatgatgctc tggcgaaaga gcccgtaacc aacaacggct gaaggcaatg
    23821 acagctcaac gaatccctga ttaagcgggc ccacatttgg cgcttcaaca acggctgtcg
    23881 cgcggcgacc tagactagcg ttaatcgacg aactgcccaa ccgaggtacg atcagccgct
    23941 caccattgtc tccgacgaag tccaccgtga aatagaccgg attcgtgctc gtgttcgtaa
    24001 ggtataatgc tgaataccac cctccgccaa atgcgaattg agggagtatc tttgtcgtga
    24061 tctcattctc taatccaata ttcagactgt cgtaaatcgc attgtccaca gggaaaccaa
    24121 gcgttcggaa atcgagtctc aggtcgcgtc cgacgccgta tgaaatagcg gcggccatga
    24181 gtgtggagcg gaacgtgtcg gcctgcaccg tgttttcgct tggggcgtag ctgctctgcc
    24241 actgcgtatt gaatcgctgg agaaacttgg tcaacctctt gacaggaatc ctgtagccgg
    24301 ttcctgcttg ctcgacgttg gcgaggaaca gataggcaag cgtcatgaag gtttgaagtg
    24361 tttcgtccgt gggagtggat gggttgttcc atgtcgcaaa tggcttgccc gtgcgcaagt
    24421 agttctcata gttctggcgg accacactta ttgtcgtgcg ggcatcctgc gcaagttcga
    24481 aggcaatttc agagctcagc ccccatgcag ttgagttgtt gacgacttca tgaatgacgg
    24541 cgtgctggaa tatctgggcc attgtttctg aatagatggc atttgcattc ccatcgactc
    24601 ttccgccgta gatgaatcca gatggaaagt tcagtgtaaa gttatgacca atttcatgcg
    24661 ctagtgacga ccagggaatt gcagcagcga atccgtgatt gcccgctgcg atgctcactg
    24721 gatttgcgta ccatatactg tagaagccac cggccgtgtc cttccatacc tgctgccgga
    24781 atgctccatt aataagcgtt atgtcgtcgt tgatcagatc cttttgggcc gctgcaatcg
    24841 ccgacaacac gtactcgatt gttactctcg gcgtcgcact gaagtccgtt gttgcgaagg
    24901 gcgccagtgt tggcagcaca gcatacagca agcggcttgt aggtagctgc cctgatagtg
    24961 caaccgtgac gtcaaatccg gagggtaggg gaacgggctg aatggctggt ggcaccaagc
    25021 gggtaagtgc ttgtgcgcga tccgtctcac ccccgccgta attcgcggcg accgtgacga
    25081 tatagttctt cacttgtgca taacggtgct gctgcgggaa gaatcctgac gtcgtttggc
    25141 catctcccca atcgaacgta aatggagtgc tcggttgcct cgtatccccg ccgttgatta
    25201 caactacgcc tgtggcagaa tcggcggccg tcactgcaat tgaaagcgaa ggcggagaac
    25261 cgagcaactc cctgcggtag tcaaacgcaa agtgctgatc ggcgaaggcc ggaacgacca
    25321 acaggaagag atagcagtag cgcagcatac gaaagacatg atatgcgatg tccgcgtcta
    25381 ccggtactgg tgcagccgcc tacgcatgct ttgggcgtgg actgcggctt cgcgaacgag
    25441 gagcagctac gc
//
LOCUS       DASTMK010000187        21391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_10288, whole genome shotgun sequence.
ACCESSION   DASTMK010000187 DASTMK010000000
VERSION     DASTMK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21391
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10288"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFQ79_08110"
     CDS             <1..1161
                     /locus_tag="VFQ79_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU0139658.1"
                     /translation="PFVTDQHVPQFTVIDAPGFRADSAGDGTNSDVFIIVNFSKKLII
                     IGGTSYAGEIKKGIFSVLNYFLPARGVLPMHCSANVGASGDTALFFGLSGTGKTTLSA
                     DPGRRLIGDDEHGWSGRGVFNFEGGCYAKCIRLNKETEPQIWNAVRFGTVLENVAVDT
                     EWRLLDYDDASLTENTRAAYPVTFIEDAIVPGIGDHPKNIVFLTLDAFGVLPPVARLS
                     AEQAMYHFLSGYTAKVAGTERGLSKDPEATFSACFGAPFLPRSPQVYAELLGERVRKH
                     QARCWLINTGWSGGGFGVGKRMSLPVTRAMVSAALGGQLDRVSYRPDPTFRIEVPGEC
                     PGVDPKLLNPRETWSDPAAYDRAARDLAGRFRQNFEKFGIGGEIRAAEPVLA"
     gene            1210..2853
                     /locus_tag="VFQ79_08115"
     CDS             1210..2853
                     /locus_tag="VFQ79_08115"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEU0139659.1"
                     /translation="MKEALVSVLTEYPILTLFIVIGLGYLVGELSLFGFRFGVAGVLF
                     VGLAVGSLSPGIALPEIVSTLGLIIFVYTIGIQSGPAFFDSFRKKGYRDSLLAVGVLS
                     FGAALTIALALLIPIAGPRAAGLYCGALTNTPALAAAREGVLQQAKNNGATEEQARRM
                     ADEPTVAYSIAYPFGVIGVLLCFELMRRVWRIRLEPTDEAGDIAVKDFIVKNPGVIDR
                     TVGDVLRLHKEPGFVISRIRQGGETGLVTSGTRLNLGDVVAVVGDEEALERARQIFGE
                     PSATQIELDRTELDYRRVFVSSKEVVGKRIRELDLQNKFQATITRLRRGDVDVVPTPD
                     TRLELGDRVRVLTHRSNFHIITRLFGDSIRGTAETDFGSVSLGLVLGVMVGMFPIPLP
                     GGTIIRLGLAGGPLLTALVLGKLERTGRVTWAIPISANLTLRQIGLLLFLGGVGTKAG
                     YSFASTIQDRGLILLGGGAAITFGVTLATMIFGYKVLKIPFGTLMGIMSGVQTQPAAL
                     AYAVNAARSDAPNIGYASVYPAAMIAKIMLAQFLVSMLA"
     gene            complement(2890..3138)
                     /locus_tag="VFQ79_08120"
     CDS             complement(2890..3138)
                     /locus_tag="VFQ79_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139660.1"
                     /translation="MPDLTETQKSILWTVAQHERREPGSLVDADDLADYVASSTTEIQ
                     REIQGLIGRGLLANAESEEEAPLRLTPAGWKAVQHFEA"
     gene            3274..3846
                     /locus_tag="VFQ79_08125"
     CDS             3274..3846
                     /locus_tag="VFQ79_08125"
                     /inference="COORDINATES: protein motif:HMM:NF022736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 family protein"
                     /protein_id="HEU0139661.1"
                     /translation="MGVPVTRYSPSSRYVVAGLVALGSAAISAWLGLQFTPALIPAVL
                     FLASAAFFLLLAFRPVVEIQESQLMVGRRSIRWEDIRRVDHTTWWSLLVVQLTLSGDR
                     QLRIVYPGDLDAANSLLRNLRRLARTALIDGVPYRRFWGEEEQATEPKQYASPKYHLL
                     RPQDEEEVERLYQRLKAVGHLDPKNSTDEN"
     gene            3850..4449
                     /locus_tag="VFQ79_08130"
     CDS             3850..4449
                     /locus_tag="VFQ79_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU0139662.1"
                     /translation="MKAPRSWRFWIVIPVAIILLGYMSAPFWLSAAGGWLVDAEPPTP
                     ADVVVVLAGDRTGSRILRAAELVKQGYAPLVLVSGPYGMYGRSEDELAIGMAVANGYP
                     ASFFAGVPHVSHSTREEAGYLVAELKKRGARRVLLVTTNFHTGRAKSVYRELGQGLDI
                     RCVSAPDPFFSPENWWRNREGRKIFFFEVTKRLADAFGI"
     gene            4452..6194
                     /locus_tag="VFQ79_08135"
     CDS             4452..6194
                     /locus_tag="VFQ79_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139663.1"
                     /translation="MRNTLRMTWPYLWRYRRGLGWGFGALLLKDLTAAALPLTIKAGI
                     DSLTQGFHLQAVFWFAALLVGIAAIKGFFQYWMRVIIISISRDIEYDLRNDLFRHLTR
                     LSSGFYSRHRTGDIMARATNDLNAVRMMFGPGIMYWTETSLTFGLALIVMLAIDWRLT
                     LMALSPAPLVSIAVVFFGRRIHDRFEVIQGMFSDISSRVQENLAGVRVIRAYVQEDAE
                     LRTFEVLNRDYIDQNIRLVRLSGVFMPLLQALIGLTFLILLWVGGLQLMRGQLTLGTF
                     VMFNTYLSMLIWPMIAFGWVVNLMQRGNASLKRIAEFMREVPDIAAPDSPKLLPSPFQ
                     GRIEFRSVTVRYDGMPALSGIDLTIPAGATVAVVGHTGSGKTSLVRLVPRLMDPSEGA
                     VLLDGVDVREFSPADVRSVIGMVPQETFLFSATLAENIAFGVKEATDEQIRHAAELAG
                     LATDIEAFPEGFQTMVGERGITLSGGQKQRTAIARAILRNPKILILDDALSSVDTVTE
                     ERILTGLGRLMHGRTTILISHRVSTVRQADRIYVLDAGRIVEQGTHSELIELGGYYAE
                     LYQRQLLEEELEAI"
     gene            6330..7304
                     /locus_tag="VFQ79_08140"
     CDS             6330..7304
                     /locus_tag="VFQ79_08140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0139664.1"
                     /translation="MTNKSRETMRAVAIDEFGGIEKMKPRELPVPQVAADEILVRVDT
                     AGVGVWDPFEREGGFAKEFGLRANFPYVLGSDGAGAVEEVGGQVHNFKRGDRVYGTSL
                     MSPKGGFYAEYTVVKANNAAVIPQALSTRDAGVLAIDGVTALDGLDKTLRLKAGESIL
                     ILGASGGIGHLAVQLAKRMKARVLAVASGQDGVEFVKQLGADKVIDGHRDDILKAARE
                     FEPKGLDAALLTAGGKAAEQAIAALGEGGRAAYPNGVGDVPQERDGVKVMSYNGEPDP
                     KTFAKLNELIDDGPFEVHIARTFRLEEAAEAHRTLESHYLGKLALVLS"
     gene            7406..8230
                     /locus_tag="VFQ79_08145"
     CDS             7406..8230
                     /locus_tag="VFQ79_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0139665.1"
                     /translation="MKSSRRVFLGGSLAVAGLPAPVLAFAGKQPGGIRLGVATYSLRK
                     FSRPEAIGILKKLGVEYVSIKEFHLHYAESPEQTEEGAREFRNAGFVVLSGGNISLQK
                     PAELRTMFDYAKAAGMPMMVCAPTHETLPEVERLVKEYDIKAAIHNHGPEDKHFPTPQ
                     SVLKAVKNMDARMGLCLDIGHTARTGADIIEAVREAGPRLFDIHVKDLQSTTDKATQV
                     PVGDGVLPVAAMFKTLRAGNYKGAVMLEYEIDADNPFQGMQKSLAYMRGVLAGLAA"
     gene            complement(8318..9211)
                     /locus_tag="VFQ79_08150"
     CDS             complement(8318..9211)
                     /locus_tag="VFQ79_08150"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEU0139666.1"
                     /translation="MALGRLLYYEPRLSKSQTISCNTCHKLDAYGVDGEPTSDGHMGQ
                     QGDRNSPTVYNAAGHFVQFWDGRAPDVEEQAKGPILNPVEMAMDSEEDVVVVLRSIPE
                     YVEVFQRAFPGEKNPVTFDNAARAIGAFERGLVTPSRWDRFLRGDRNALTSEEQAGFK
                     TFTSIGCQACHMGAFVGGSIYQKLGVYEPWPDSKDPGRYKVTQNEGDWMIFKVASLRN
                     VEKTGPYFHNGKVQTLEEAVSLMADHQLGKKLKESEVRAILAWLKTLTGEIPAEYIKP
                     PVLPRSTSATPPPNRGLHTSD"
     gene            complement(9426..9890)
                     /locus_tag="VFQ79_08155"
     CDS             complement(9426..9890)
                     /locus_tag="VFQ79_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139667.1"
                     /translation="MLSRLINLISDHWEVIATRVIRQMRRNADLLEMGKLSERDLTER
                     TREILTQLPTWLISSEAEVASHYELLGRRRSAEGIPLYEVVRALQLIKESAIAYIRDQ
                     GMARSTVDLYTSEEFEHDMGRMFDKMIFSVVRGYEKSMRAAPEGVKPAASQV"
     gene            complement(9953..11209)
                     /gene="lysA"
                     /locus_tag="VFQ79_08160"
     CDS             complement(9953..11209)
                     /gene="lysA"
                     /locus_tag="VFQ79_08160"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU0139668.1"
                     /translation="MDFLGKSFSYRQGSLYCGNTRLSDVADEVGTPCYLYSAPDIVSK
                     FNAYAAGLEGQRHRVCYAVKANSNLAVLGLLARAGAGFDIVSGGELFRVLKAGGNASA
                     VVFSGVGKTADEIDYALREGIHSFNCESEAELTLIDAKAARLGKRAAVALRVNPDVNA
                     ATHPYISTGLREHKFGIPISQVEDVYERAKSLRNIDPAGVSCHIGSQLLETGPLLEAL
                     DALLALVDRLSSRGIAVRYLDVGGGLGVPYKAHQKSPCPTGYAGRVRERVAGRDLEIM
                     LEPGRSIVAEAGVLLSRVLYRKQTGGKEFVIVDAAMNDMLRPALYSAHHEIIPVRQKG
                     FGTVHADVVGPVCETGDFLAREREIDNVFPGDLVAICTAGAYGFVSASNYNARPRPAE
                     VLVENDGWRVVRERETYEDLIRGERI"
     gene            complement(11225..12973)
                     /locus_tag="VFQ79_08165"
     CDS             complement(11225..12973)
                     /locus_tag="VFQ79_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU0139669.1"
                     /translation="MADASITPIHFSERAELLDFLLEVVEATSSTLDLDRLLANVAGI
                     VKKVIPYDLLAILLYTERLPGLRIRYAIGHRPEIVNNLVVPLGEGLTGTAAATREPVL
                     VQEVRNDERYLNALDAVRSELAVPMVARGKLVGVIDLQAIRPGAFTDYDRTLLRLIAS
                     RVAVSIDNARLYRRVERQNRTLRTLATLSQEFTSILDLDQLLRKIATRVRSLINYDAF
                     SILLVDSKRQALRHRFSMRYDERVELDNIAIGKGITGAAVESRETIRVVDTLADPRYI
                     ASHPDIRAEVAVPLIVQDNVIGVMDLESSRVGHFTDDHVRTLSLLAPQIAISVENASL
                     YEKLAQRERRMEQDLKAARKLQSVLMPRKPPEIAGVDIGIARRAALEISGDIYDFFEQ
                     DEHAIIAFGDSSGKGAAAALYGALVSGLLRSLAPRRRAPSLLLRAINEILLERKVDAQ
                     YVTLLVLLWEARTRQITMANAGAVPPIICRGEEVLRPRVEGVPVGLLDDREYEEYRVE
                     AQSGDVLVLPSDGVLDQWNAAGEEYGKDRLAKVLREARDYPPQTIVDMILDDVDRHAG
                     GTRIFDDQTVIVVKVK"
     gene            complement(12982..13623)
                     /gene="deoC"
                     /locus_tag="VFQ79_08170"
     CDS             complement(12982..13623)
                     /gene="deoC"
                     /locus_tag="VFQ79_08170"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU0139670.1"
                     /translation="MIDHTVLNPGATRADIVQVCREARQYGFASVCVNPYWVGLAAAE
                     LTGSAAAVSTVCGFPFGAGTTEAKRTEAAAVLRTGAQEIDMVLNVGALRGGDHDSVRL
                     DIRAVAEICHNAGALLKVILETALLDDEQKTIACKLAMMAGADFVKTSTGFGPAGATV
                     HDVSLMRSVVGPEVGVKAAGGIRTLDDLKKMAAAGANRMGTSASVRIIEATAG"
     gene            14187..16781
                     /locus_tag="VFQ79_08175"
     CDS             14187..16781
                     /locus_tag="VFQ79_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU0139671.1"
                     /translation="MQDQDQDVIREFLVESHENLSRLDQELVELEKRPKDAVLLAGIF
                     RTIHTIKGTCGFLAFSTLEAITHQAESLLSQLRDGQRELNPSLVTLILDTVDATRKVL
                     ASIEATGEEGPDRFGELTERLRAAAQAPAGSKGQQASGKLPDPVEPPDNNDRLEHPEV
                     TKPEEEAAKSSAVVDANIRVGVGLLDKLMDLVGELVLTRNQILQFNTEREDAALNATS
                     QRLNLITTELQEGVMKTRMQPIGMVWNKLPRVVRDMAVALGKQIRLEMDGAGTELDRT
                     IIEAIKDPLVHLVRNACDHGIEPPDVRVRAGKPAQGRLTLRAYHEGGQVNIEIGDDGA
                     GVDVTRVKQKAIEKGLLRPEQAEKLSDREAVNLIFQAGFSTAQTVTNVSGRGVGMDVV
                     KSHIEKIGGIVDVFSRPGEGSTVKLKIPLTLAIIPGLVITSGGERFVIPQVSLLELIR
                     LEGDSKEKHIEHVHGTPVYRRRGSLLPIAYLNEVLGLSSAERAEAVSMVVLQAEDRQF
                     GLVVDGINDTQEIVVKPLGKQLKGLTVYAGATIMGDGRVALILDVLGIGQRSGVLAES
                     REPARAAAEQKTQSEIEQQRLLLFRAGSFDRLAVPLSLVARLEEFPRSAIEYAGGCQV
                     VQYRNRILPLVPLRAVLESGARDQDHSADPAQVIVFNDGDRSVGVMVDQILDVAEEAV
                     TVRQKTGREGLLGSAVVGKRVTDFLDLNHVICMAAKNWFQGVDGSAAGKNILVVEASA
                     FFRGLIRTNLDMAGYRVREAANLDEAIRVLEQQPVDIVVAALDLPPSGSSALSAAMRR
                     RPEWRKIPILELADSTEQVRTRASQSADFQDCQMKFNREAMLESVARLASALASADAS
                     PVLVGEEK"
     gene            16785..17264
                     /locus_tag="VFQ79_08180"
     CDS             16785..17264
                     /locus_tag="VFQ79_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU0139672.1"
                     /translation="MATEPATMRSEPAQTGGQFATFFVADLFFGVDVLRVQEVLRFQQ
                     MTRVPQAPEVIEGLINLRGQIVTAIDMRRRLGLPPRAGDQAPMNMVVRTEDGAVSLLV
                     DEIGDVLDVDAATHERPPENLDPAARELIRDVYKLKDRLLLILDAERTVDLAVERAA"
     gene            17373..19244
                     /locus_tag="VFQ79_08185"
     CDS             17373..19244
                     /locus_tag="VFQ79_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0139673.1"
                     /translation="MRKQVNGGRRIRPDRNPSISTAVLDRPVPSAPRIQTKDRLGEEI
                     LRVTSALQQGGLSERVRADLFEGGDRIIVDGVNGMLDAVIEPLNVAVGFLARVNRGDE
                     LHKITQEYQGDFQVLKDNINSVISRLYGLIDETQRRSQAGINGEVGTRADLSNYPGGW
                     GRIIKGFNDALDAFTGPLTVAAEYVDRISKGDIPPKITDTYNGDFNEIKNNLNTCVDS
                     LNSLIAEMKRMSVEHEKGDIDVTIPAGKFQGAYQVMAQEVNDMVAGHISDTRKAVACV
                     AEFGKGNFEAPLEKFPGKKGLLNDTIEQVRTNLKALIADTDTLVKAAVEGKLGTRADA
                     SKHLGDFRRIVEGINKTLEMVVEPLKVTAQNATALASSSEELTAVSHQMAGNAEETAT
                     QANVVSAASEQVSNNVSSVASASEQMQASIREIAKNANESARVAKNAVSVAHSTNTTV
                     TKLGESSQEIGNVIKVITSIAQQTNLLALNATIEAARAGEAGKGFAVVANEVKELAKQ
                     TAKATEDIGRKIEAIQGDTKGAVKAIEEISTIINQVNDISNSIASAVEEQTVTTNEIG
                     RSVTEAAKGVGDIAKNIGGVAVAAKNTTQGANDTQKASQELSQMAAQLQTVISKFTF"
     gene            19323..19697
                     /locus_tag="VFQ79_08190"
     CDS             19323..19697
                     /locus_tag="VFQ79_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0139674.1"
                     /translation="MAKALVVDDSKAVRMILAKILRELGYEVREAENGRGALEVIEAE
                     KNAVALVLADWNMPEINGLDLLKRLRQNPELSSLVVVMVTTETGLDQMMAALEAGANE
                     YVMKPFTKDILVEKLQLAGIHP"
     gene            19708..20805
                     /locus_tag="VFQ79_08195"
     CDS             19708..20805
                     /locus_tag="VFQ79_08195"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEU0139675.1"
                     /translation="MAAINKRLLQPGERIRVLVVDDSVVIRRLVTHALEQHPSVEVVG
                     TAANGVIALQKIPQVNPDVLTLDIEMPEMDGLETLRRIRREYPHLRVIMFSTLTERGG
                     AVTLEALSLGADDYVAKASNEGSLDRSMARLREELVPKIKQFFHIPALNSAVARPAPG
                     QAPVPPPIWRSTKVRPKVVVIGVSTGGPTALGEILPELPAGFPLPVLVVQHMPPLFTR
                     LLAERLHPICQLPVEEGSQGALVDAGSILIAPGDFHMKVASNGDRVRIHLDQSPPQNS
                     CRPAVDALFTSIGEVYGGAVIAVILTGMGQDGLRGTRILKAQGASVLAQDEASSVVWG
                     MPGAVVNANLADRVVPLDQVVPEILRIVGRA"
     gene            20816..>21391
                     /locus_tag="VFQ79_08200"
     CDS             20816..>21391
                     /locus_tag="VFQ79_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEU0139676.1"
                     /translation="MPSQTSIAEIRSENYRFLQQHVYSHAGIVLEEDKHYLFESRLAP
                     IVKQLGLNSINDLCTLLMATRETQVGRQVVEAMTTNETYFFRDPAQYDAIRTVLLPRL
                     RRERQNMRTLRFWSAAASTGQEAYSLAMVLLEDGFSDWNIQILGTDLSSPVLERARSG
                     KYQQIEVNRGLPAALLVKYFRRSGMDWHLSEA"
BASE COUNT         4375 a         6177 c         6515 g         4324 t
ORIGIN      
        1 ccgtttgtga ccgaccagca cgtcccccaa ttcacggtta tcgatgcgcc gggattccgc
       61 gccgattccg ccggagatgg caccaactcc gatgtcttca tcatcgtgaa cttctcgaag
      121 aagctgatta tcatcggagg aacttcttat gcgggtgaga taaagaaggg catcttcagc
      181 gtgttgaatt atttcctgcc ggcgcggggc gttctgccga tgcactgttc ggcgaacgta
      241 ggagcatccg gggatacagc ccttttcttc gggctctcgg gcaccggtaa gacaacgctg
      301 tccgccgacc ccggcagacg gcttatcggt gacgacgagc atggatggag cggccgtggt
      361 gttttcaatt tcgaaggcgg ctgctatgcg aaatgcatcc ggctcaataa agaaacggag
      421 ccgcaaattt ggaatgccgt ccgcttcgga acggttctcg agaacgtcgc cgtcgacacg
      481 gagtggcgtt tgctggacta tgacgatgct tcgttgaccg aaaatacacg ggccgcctat
      541 ccggtgacct tcatcgagga cgcgattgtc ccgggcatcg gagaccatcc gaagaacatt
      601 gttttcctga cgcttgacgc gtttggggta ctacctcccg tcgcgcgttt gtccgccgaa
      661 caagccatgt accacttctt gagtggttat accgcgaagg ttgcaggcac cgaacgcggc
      721 ttgagcaaag atccggaagc gacattcagc gcgtgtttcg gcgcgccttt tcttccgcgc
      781 agcccccagg tatatgcaga attactgggt gaacgggtgc ggaagcatca agccagatgc
      841 tggttgatca ataccggctg gtccggcgga gggttcggcg tcggaaagcg tatgagtctg
      901 ccggtcactc gcgccatggt gagcgccgcc ctcggcggac aattggatcg ggtgtcttac
      961 cggccggatc cgacgttccg tattgaggtg ccaggggagt gtcctggagt ggatccgaaa
     1021 cttctgaacc cgcgtgagac ctggtccgat ccggcggctt acgaccgggc agcgcgagac
     1081 ctggccggcc ggttccgcca gaacttcgaa aaattcggta tcggaggcga aattcgcgcc
     1141 gccgagccgg ttctcgctta aagtctgata ctaggggcag atgcacaacg cgcctgcccc
     1201 tccaccgaca tgaaagaagc actcgtcagt gttcttactg agtacccgat cctcacgctg
     1261 tttatcgtta tcgggctcgg atacctggta ggtgagctga gcctgttcgg attccggttc
     1321 ggagtggccg gagtattgtt cgtcggcttg gccgtaggtt ctctcagtcc cggcatcgcc
     1381 ttacccgaaa ttgtctcaac gctggggctg atcatcttcg tatatacgat cggcattcaa
     1441 tccggcccgg cgtttttcga ctcgttccga aaaaagggat atagggacag ccttctcgcc
     1501 gtgggcgtgc tctccttcgg cgcggcgctc acaattgcac ttgctctcct gattccgatc
     1561 gccggcccgc gcgcggccgg tttgtattgc ggagcgttga ccaatacgcc ggcgctggcc
     1621 gccgcgcgcg aaggggtttt gcagcaagcc aaaaacaacg gggcaacgga agaacaggcg
     1681 cgccggatgg ccgacgagcc gacggtggcc tatagcattg cgtatccctt tggcgtcatc
     1741 ggggtgttgc tgtgttttga gctcatgagg cgcgtctggc gcatccggtt ggagcccacc
     1801 gatgaagcgg gcgacatcgc ggtgaaggat ttcattgtca aaaaccccgg cgtgattgat
     1861 cgcacggtgg gggacgtatt gcgtctgcac aaagagcctg gtttcgtcat cagccgcatc
     1921 cggcagggcg gcgagaccgg cctggtgaca tccggcaccc gcctgaacct gggcgacgtt
     1981 gtggcggtag tgggagatga ggaagccctg gagcgggccc gtcaaatttt cggtgagccg
     2041 agcgccacgc agatcgaact ggaccgcacc gagctggact accggcgcgt ctttgtctcc
     2101 agcaaggaag tagtgggcaa gcggatccgt gaattggatc tacagaataa attccaggcc
     2161 accatcactc gtctgcggcg cggcgatgtg gatgtagtgc caactcccga cacccgcctg
     2221 gagttgggag accgggtgcg ggtgctgacg catcgcagca atttccatat catcacgcgc
     2281 ctgttcggcg attccatacg aggcaccgcc gagaccgact ttggctccgt gtcgctcggc
     2341 ctcgtccttg gcgtcatggt ggggatgttc ccgattccgc tgccgggtgg aacgatcata
     2401 cgtctgggcc tggccggcgg tccgttgctg acggcgctgg tgctagggaa actcgagcgg
     2461 acgggacgcg tcacgtgggc cattcccatc agtgcaaacc ttacactgcg gcaaatcgga
     2521 ctgctgctct tcctgggcgg tgtcggaacg aaggctggtt attccttcgc cagcaccatt
     2581 caggaccggg ggctgatcct gctgggcggc ggcgccgcca tcacctttgg ggtaacgctg
     2641 gccacgatga tcttcggata caaagtccta aaaattccgt tcggtacgct gatggggatc
     2701 atgtccggtg tgcagacaca gcccgccgcg ctagcttacg ccgtgaatgc cgccaggtcg
     2761 gacgctccca atatcggcta tgccagcgtc tatccggcgg cgatgattgc caagattatg
     2821 ctggcgcagt tcctggtcag catgcttgcg tgacgcgcga cttatccggc gtcgcttggt
     2881 taccgtgtac taagcttcaa agtgctgaac ggccttccag ccggccgggg tgagccggag
     2941 aggggcttcc tcttcactct cggcatttgc gagcaggcct cttccgatca gcccttgaat
     3001 ctccctctgg atttccgtgg tgctggaggc cacgtaatct gccaggtcgt cagcgtctac
     3061 gagagaaccc ggctcgcgcc gttcatgctg ggcaaccgtc cacagtatcg atttctgggt
     3121 ctcagtcaga tccggcatta ccaatctccc tgtctgtctt accacaaaac gcagccggaa
     3181 aagcggattg cgcggatgcc gtataccgcg gcataggaac cacggaaatt acccgcgttg
     3241 acgccgtcga agacccggcg ttagtctaaa ggtgtgggag ttccggtaac acgctattcc
     3301 ccctcgagcc ggtatgttgt ggccggtctc gtggcgttgg gctcagcggc tatttcggcg
     3361 tggctcggtc tgcaatttac gccggcactg atccccgcag tcctctttct ggccagcgcc
     3421 gcgtttttct tgcttctcgc tttccgccct gttgttgaga tacaggaatc acagcttatg
     3481 gtgggacggc gcagcatccg atgggaggac atccggcgag tcgatcacac cacctggtgg
     3541 tctcttctcg ttgttcagct tacactctcc ggcgaccgcc agctcaggat cgtgtatcct
     3601 ggcgacctcg atgcggcaaa cagcctgctg agaaaccttc gccgtctcgc acgcacggct
     3661 ttgatagacg gcgttccgta ccgccgcttc tggggtgagg aggagcaagc taccgaaccg
     3721 aagcagtatg cctcgccgaa gtatcatctg ctccggcccc aggatgaaga ggaagtcgaa
     3781 cggctgtacc aacgtctgaa ggccgtcggc catctcgacc caaagaactc gacggacgag
     3841 aattagttct tgaaagcccc gcgctcatgg cgtttctgga ttgtgattcc ggtagcaatc
     3901 attcttctcg ggtacatgag tgcacccttc tggctcagcg cggcgggcgg ttggctggtt
     3961 gatgcggaac ctcccactcc cgcggatgtc gtggtagtcc tcgccggcga ccggaccgga
     4021 tccagaattc tgcgagcagc cgaactggta aaacaaggct acgctccgct ggtgctcgtg
     4081 agtgggcctt atggaatgta cggccgctcg gaagacgagc ttgccatcgg aatggccgtt
     4141 gccaacggat acccggcgag cttcttcgcg ggcgttcccc atgtgtccca ttccacacgc
     4201 gaggaagcag gttacctcgt tgccgaactg aagaagcgcg gagccaggcg tgtcctgctg
     4261 gttaccacca actttcatac gggccgcgcc aaatccgtgt atcgggaact cgggcaagga
     4321 ctggacattc gctgtgtgtc agcacccgat cccttctttt cacccgaaaa ctggtggcgc
     4381 aatcgtgaag gccggaagat ctttttcttt gaggtcacta agcggctcgc ggatgcgttc
     4441 ggaatctagg aatgcgcaac acccttcgaa tgacctggcc ttatctctgg cgctaccggc
     4501 gtggactcgg atggggcttc ggcgccctcc tgcttaaaga tctgacggcc gccgcacttc
     4561 cgctgacgat taaagcgggc atcgactcgt taacccaggg gttccacctt caggccgtgt
     4621 tctggtttgc ggcgctactg gtcggcatcg cggcgatcaa gggctttttc caatattgga
     4681 tgcgtgtgat catcatcagc atctcgcgcg acattgaata cgatcttcgc aacgacctgt
     4741 tccggcactt gacgcggctg tcgtccggct tctattcgcg ccaccgcacc ggcgacatca
     4801 tggctcgcgc taccaatgac ctgaacgcgg tccggatgat gttcgggccc ggaatcatgt
     4861 attggaccga aacttccctg acattcggtc tggcgttgat tgtgatgttg gcgatcgact
     4921 ggcggctgac cctgatggcg ctgtctccgg cgccactggt aagtatcgcg gtggtgtttt
     4981 tcggccgccg catccacgac cgcttcgaag tcatccaggg gatgttttcc gatatcagca
     5041 gccgtgtcca ggaaaatctg gcgggggttc gcgtgattcg cgcttacgtt caggaagatg
     5101 cggagttgag gacgttcgag gtcctgaacc gcgactatat cgaccagaac atccgcctgg
     5161 tgcggctgtc gggcgtgttt atgccgctac tgcaggccct gatcggattg accttcctga
     5221 ttctgctctg ggttggcggc ctgcagttga tgcgtggcca gcttacgctc ggcacgttcg
     5281 tcatgttcaa cacctatctc agcatgttga tctggcccat gatcgccttc ggctgggtgg
     5341 taaacctcat gcagaggggg aacgccagct tgaagcgcat tgccgaattc atgcgtgagg
     5401 taccggacat cgccgcgccc gattcgccaa agctgctgcc ttctccgttc cagggccgca
     5461 tcgaattccg ctccgtgacg gtacgctatg acggcatgcc cgcgctttcc ggaatcgatc
     5521 tcaccattcc cgcgggcgca acggttgccg ttgttgggca caccggctct ggaaagacca
     5581 gccttgtgcg gctggtgccg agattgatgg acccaagcga aggcgctgtc ctgttggacg
     5641 gagtcgatgt gcgggagttt tcgcccgccg acgtgcgcag cgttatcggc atggttccgc
     5701 aggagacctt tcttttcagc gcgacgctgg ccgaaaacat cgcatttgga gtgaaagagg
     5761 cgacggacga acagatccgc catgcggcgg agctggcggg gcttgccacc gatattgaag
     5821 cctttccgga agggtttcaa accatggtgg gcgagcgggg catcaccctc tcgggaggcc
     5881 agaagcagcg cacggccatc gcccgggcca ttcttcgcaa tccgaaaatt ctgatcctcg
     5941 atgacgcgct gtcgagcgtg gatacggtga cggaggagcg cattctcacc ggactcggcc
     6001 gcctgatgca tggacgcacc accattctca tttcccaccg tgtttccacc gtgcggcagg
     6061 cagacaggat ctatgtgctc gacgccggcc gaatcgtcga acagggcaca cactccgagt
     6121 tgatcgaact cggcggttac tatgcggaac tctatcagcg gcagttgctc gaagaagagc
     6181 tcgaagccat ctgacgggtc actattggcc accccggcgc ggcgctgatt cagcatgcac
     6241 gagttcgtgt aacataacct ccctccggat tgtcaatacc tgagaaaggc aattatcagc
     6301 atggcatcgc gtgcggaaag gagatgctca tgacaaacaa atcacgtgaa acgatgcggg
     6361 cggtggcgat cgatgaattt ggggggatcg agaaaatgaa gccgcgagaa ttgcctgtgc
     6421 cgcaggtggc tgccgatgag atcctcgtcc gggtggatac tgcgggggtt ggcgtctggg
     6481 atccgtttga gcgcgagggc gggttcgcta aagagttcgg cttgcgagcc aactttcctt
     6541 atgtgctggg gtcggacggc gcgggagctg tggaagaggt gggcggtcag gttcataatt
     6601 tcaaacgcgg cgaccgagtt tacggcacta gtttgatgag tcccaagggt ggcttttatg
     6661 cggaatacac ggtggtcaaa gccaataacg cggcggtgat cccgcaggca ttgtctacac
     6721 gagatgccgg tgtcttggcc atcgatggcg tcaccgcgtt ggatggtctg gataaaacgc
     6781 ttaggcttaa agccggtgaa tcgattctga tcctcggtgc cagcggcggt atcggccatt
     6841 tagcagttca actggccaag cgcatgaaag cacgggttct ggctgtggcc tcaggccagg
     6901 atggcgtgga gttcgttaag cagttgggcg ccgacaaggt tatcgacggg catcgagacg
     6961 atattctcaa agcggcgcgt gaatttgaac cgaagggcct ggatgccgct ttgttgacgg
     7021 ctggcggaaa agcggctgag caggcgattg cggcactagg ggaaggtggc cgagccgctt
     7081 acccgaacgg cgtgggggac gttccccagg agcgtgatgg cgttaaggtg atgagctata
     7141 acggagagcc tgacccgaag acgtttgcca agctgaatga actgatcgat gacggaccgt
     7201 ttgaagtcca cattgcgcga acctttaggt tagaagaggc ggccgaggcc caccgaaccc
     7261 ttgaaagtca ttacctcggc aagctcgctc ttgttctgag ctgactggaa tgaagacccg
     7321 ctgatctttc ataatcgata ccggtccaag agctactata gcggcgctag aatagggatt
     7381 cacggctcag gaggagcaaa ccactatgaa atcgtcgcga cgtgtctttc ttggaggaag
     7441 cctcgccgtc gccgggctgc ccgcgccggt gctggcgttt gccggcaaac agcctggcgg
     7501 cattcggttg ggcgttgcaa cctattcgtt gcgcaagttc tcccgcccgg aagcgatcgg
     7561 cattttaaag aaactcggcg ttgagtacgt cagcatcaaa gaatttcatc tccactatgc
     7621 ggaaagcccg gaacagactg aggaaggagc cagggagttt cgcaatgccg gatttgtcgt
     7681 tctgagcggc ggtaatatct cactccagaa accggccgag ttgaggacga tgttcgatta
     7741 cgccaaagcg gccggtatgc ccatgatggt ctgcgcgccc acgcatgaga ctctgccgga
     7801 agtggaacgg ctggtgaagg agtacgacat caaagcggcg atccacaacc atgggccgga
     7861 ggacaagcac tttccaactc cgcagagcgt gttgaaggct gtgaagaaca tggatgcgcg
     7921 catggggttg tgtctcgata ttggccacac cgccagaacg ggcgccgata tcatcgaagc
     7981 cgtccgcgaa gccgggccgc gcctgttcga cattcacgtg aaagacctcc aaagcacaac
     8041 ggataaggct acccaggttc ctgtcggcga cggcgtcctg ccggtcgccg ccatgttcaa
     8101 gacgcttaga gcggggaatt acaagggcgc tgtcatgctg gagtatgaaa tcgacgcgga
     8161 taatccgttc cagggaatgc agaagtccct tgcttacatg cgcggtgtgc tggccggcct
     8221 ggcggcctga gacgctttaa gagcctgttg aaaaatgtag aaaaccgctt gctggcgcgc
     8281 gcggctcaga atcggagccg tgaccgtcag ggagcggtca atccgacgtg tgcaggcccc
     8341 ggttgggcgg cggagtggcg gatgtgcttc gaggcagcac cggcggcttg atgtattcgg
     8401 ccgggatctc gccggtaaga gtcttcaacc atgccaggat cgctcttacc tcgctttctt
     8461 tcagcttttt cccaagctgg tgatcggcca tcagcgacac ggcttcttcc aacgtctgta
     8521 ctttgccgtt gtggaagtat ggcccggtct tctccacgtt gcgcagtgag gcaaccttga
     8581 agatcatcca gtcgccctcg ttttgcgtca ccttgtatcg tcccgggtcc ttcgaatccg
     8641 gccatggctc gtaaacgccg agcttctgat agatactgcc tcccacgaac gcccccatat
     8701 gacatgcctg gcaccctatc gaagtgaatg ttttgaaccc agcctgctcc tcgctggtca
     8761 atgcgttccg atcgccgcga aggaaccggt cccatcgcga aggcgtgacc agtccgcgtt
     8821 cgaacgcccc gatagcccgc gcggcattgt cgaaagtcac ggggttcttc tcgcctggga
     8881 acgcacgctg gaagacctct acgtattccg ggatggagcg caacacaacg acgacatctt
     8941 cttccgagtc catcgccatt tcgacaggat tcagaatcgg gcccttcgcc tgctcctcga
     9001 cgtccggcgc tcgcccatcc cagaactgca caaaatgtcc ggcggcattg tagaccgtag
     9061 gcgaattccg atcgccttgc tgccccatgt gtccatcgga ggtcggttct ccatctactc
     9121 catatgcatc cagtttgtgg caggtgttgc aggaaatagt ctggcttttt gagagccggg
     9181 gttcgtaata cagcagacgg cccagcgcca cctttgcttc ggttgccgga ttggatttcg
     9241 cgggaagtgc ttccggaatc ggtcggaagg gacgaagatc gctggtgtct attgttccgc
     9301 tgtccttcga acaggaaaca gagcccccca ccaaaaccag aagcgcgcac agaagtaaga
     9361 aggcaggtct caacatagca gtcgaattca caacttacct gccattatct tcgattctca
     9421 cagactcaaa cctgcgaggc agccggtttt acgccctccg gcgcggcgcg catggatttc
     9481 tcatagcctc tcaccacgga gaagatcatt ttgtcgaaca tccggcccat atcatgttcg
     9541 aactcttccg acgtgtaaag gtcgacagtg ctccgcgcca tgccctggtc gcgaatgtag
     9601 gcgatcgcgc tctccttaat caattggagc gcgcgaacca cttcgtacag gggaattccc
     9661 tcggccgacc ggcgtctccc caggagctca tagtggctcg ccacctccgc ttcggaggag
     9721 atcagccagg taggcaattg cgtcaggatt tcacgcgtgc gctctgtcag gtcccgctcg
     9781 gacagcttgc ccatttcaag cagatccgca ttacgccgca tctgcctgat cacacgggta
     9841 gcaatcactt cccagtgatc cgagatcaaa ttgattagtc ttgacagcat ccgttgctcc
     9901 ccataatttc aaggtagcac cgggtggcgg gtgtccgtcg ggacagacct ggtcagatgc
     9961 gctcgccccg gatgagatcc tcataagttt cccgttcgcg cactacgcgc cagccatcat
    10021 tttctaccag gacttcggcc gggcggggac gcgcattata gttcgacgcg gatacgaagc
    10081 cgtatgcgcc ggcggtgcat atggccacca ggtcacccgg aaacacgttg tctatttccc
    10141 gttcgcgtgc cagaaagtcg cccgtttcac aaacaggccc tacaacatcg gcgtgaaccg
    10201 ttccgaagcc tttttggcgt acaggaataa tttcgtgatg cgcgctgtaa agggccggcc
    10261 gcagcatgtc attcatggcc gcgtccacga tgacgaactc tttaccgccg gtctgtttcc
    10321 ggtagaggac gcgggacagc aacacgccgg cttcggcgac gatagaccgc cccggctcca
    10381 gcataatttc cagatcgcgg cccgctaccc gttcgcgcac gcgtccggca tacccggttg
    10441 gacaaggcga tttttgatgc gctttgtaag gcacgcccag gcctccgcca acatccaggt
    10501 acctgacggc gatgccgcgc gaagagagcc ggtccaccag cgccaggagc gcgtccaagg
    10561 cttcgagcag tgggcctgtt tcgagcagtt gcgagccgat gtgacagctc accccggccg
    10621 gatcgatgtt ccgcagagat ttggcgcgtt catagacgtc ctcgacctgc gagattggga
    10681 tgccgaactt gtgctcccgc aaacctgtcg agatgtaagg atgagtagcc gcattgacat
    10741 ccgggtttac gcgaagcgcg acagcggcgc gcttgcccag acgtgcggct ttcgcgtcaa
    10801 tcagcgtgag ctcggcttcc gattcgcaat tgaagctgtg gatgccttca cggagagcgt
    10861 aatcgatctc gtccgccgtt ttgccgacgc cggagaacac cacagccgaa gcattcccgc
    10921 ccgctttcag cacgcggaac agttcgccgc cggagacaat gtcgaagccc gcgcccgcgc
    10981 gggccagcaa tcctaaaacc gccagattcg aatttgcttt tacggcgtag caaacacgat
    11041 ggcgctggcc ctccagcccc gccgcgtacg cgttgaactt gctgacaata tccggcgccg
    11101 aatagagata gcagggtgtg cccacctcat cggccacatc cgacaacctg gtattcccgc
    11161 agtaaagact tccctgccgg tacgagaacg acttgccaag aaaatccata atggaagcgc
    11221 tgatctattt gactttgaca acaatgacgg tctgatcgtc gaaaatcctt gttccaccgg
    11281 cgtgccggtc gacatcgtcc aggatcatgt cgacaatcgt ctgcggcggg taatcgcgag
    11341 cttcgcgcag aaccttcgcg aggcggtctt taccgtattc ttctccggct gcattccact
    11401 gatccagtac accgtcggaa ggcagcacca ggacatctcc ggattgcgcc tcgacccggt
    11461 attcctcata ttcgcgatcg tcgagcaacc ccacgggaac tccttccacg cgggggcgga
    11521 ggacctcttc gccccggcag atgatcgggg ggacggctcc cgcattggcc atggtgatct
    11581 ggcgggtccg ggcttcccaa agcagaacca gcagcgtcac gtactgcgcg tcgactttgc
    11641 gctcgagcag gatctcatta atggcccgga gcaacagcga gggggcgcgc cgtcggggag
    11701 ccaggcttcg gagcagtccg ctgaccagcg cgccatagag cgcggccgcc gcgcccttgc
    11761 cgctggaatc cccgaaggcg ataatggcat gctcgtcctg ttcgaagaag tcgtagatat
    11821 cgccgctgat ctccagcgcg gcccggcgcg cgatgccgat atcgacgccc gcgatctccg
    11881 gcggcttccg cggcatcaga accgattgca gtttcctcgc cgccttcagg tcctgctcca
    11941 tccggcgctc gcgttgcgcc agtttttcgt acaagctcgc gttttcgacg gagatcgcga
    12001 tctggggcgc aagcaaagag agcgtgcgga cgtgatcgtc ggtgaaatgt cccacacggc
    12061 tgctctccag gtccattaca ccgatcacgt tgtcctgcac aatcagcgga acggcaactt
    12121 cggcacggat gtcggggtgc gacgcgatgt agcgtggatc ggcgagcgta tccacgaccc
    12181 tgatcgtttc ccggctctcc accgccgctc ccgtgatgcc tttaccgatc gcgatattgt
    12241 ccagttccac ccgttcgtcg tagcgcatgc tgaaccggtg ccgcagcgcc tggcgtttac
    12301 tgtcgacgag cagaatgctg aaggcgtcgt agttgatcag cgaacgcacg cgggtggcga
    12361 tcttgcgcag caactggtcc agatcgagaa tggacgtgaa ctcctgcgag agcgtcgcca
    12421 gcgttcttag cgtccggttc tgcctctcca cgcgccggta aagacgggca ttgtcaatcg
    12481 aaaccgccac tctggatgcg atcaggcgca gtaaagtccg gtcgtaatcg gtgaaggcgc
    12541 cggggcgtat cgcctgaaga tcgataactc ctaccagctt gccgcgcgcc accatcggca
    12601 cagcgagttc cgatcttacg gcgtccaggg cattcagata ccgctcgtcg ttccggacct
    12661 cttgaaccag gaccggctcg cgagtggccg ccgccgtgcc ggttaaccct tcgcccagtg
    12721 gaacgacaag gttattcaca atctccgggc gatgtccgat ggcatagcga atccgcaagc
    12781 ccggcagtct ttcggtgtaa agcagaatgg cgagcaggtc gtacgggatg accttcttta
    12841 cgatccccgc gacattggca agcaggcgat cgaggtccag ggtcgaggac gtcgcctcga
    12901 caacttccaa aaggaaatcg agcagttcag cgcgttcgct gaaatgaatc ggcgtgatgg
    12961 acgcatcggc cataagggaa ctcaaccggc cgttgcctcg atgatccgga cactggcgct
    13021 tgtgcccatc cggttcgccc cggcggcagc catttttttt aaatcgtcga gcgtgcgaat
    13081 cccccccgcc gcttttactc ccacttccgg tccgaccacc gatcgcatca gggacacatc
    13141 gtgcacagtg gccccggcag ggccaaagcc ggtcgacgtc ttgacgaaat ccgcaccggc
    13201 catcatagcc aacttgcacg caatcgtttt ctgctcatcg tcaagaagcg ccgtctctaa
    13261 aatcactttc aaaagggctc cggcgttatg gcagatttcc gcgacggcac ggatatcaag
    13321 cctgacggaa tcgtgatcgc cgccgcgcag cgcgccgacg ttcagaacca tgtcaatctc
    13381 ctgtgcgccg gtccttagga cggcggccgc ttcagtgcgt ttggcctcag tggttcccgc
    13441 accaaacgga aacccgcaga ctgtggaaac cgccgctgcg gaaccggtta attcagcggc
    13501 agcgaggccg acccagtacg gattcacgca aacgctcgcg aagccatact gcctggcttc
    13561 ccggcagacc tgcacaatgt cggcacgtgt tgcacccgga ttaagcacgg tgtgatcgat
    13621 cacggcggcg atggctcggt cgaagccggc cgatggctca cttccggaaa ggatcggacc
    13681 agcgcttctt ggttgcgatt tcgaggagta taatcggttg cgccgcgcgg cattcccggg
    13741 cattcgcgcc agaatttcat cgccaatccg cgcggcgatc cgctctaatt ctgtggagtc
    13801 caagctgaat atcttcgttg acgtcgctgc tgttgagaac ccatcccata ccccggcccg
    13861 aatcctagct tcgagcgtaa gtatatgtta actcattctc ttcggggttt cttagcgcag
    13921 tattcatcat aacatagacc cgtcccggcg cccgctttcc cccgcgggcc ggaagcagca
    13981 acttcccgag atccgcgcgc ccggacgcgc gattcaaaga aggaaatatg taaaggattc
    14041 ccgctcttcc accgataagg taatcagtgc gagtgctcgt cgaccgccgc cgcagcaaag
    14101 tgcccgaccg tcgagagaga gcatggcgca aacccactaa aggacttcag ccaccgcctg
    14161 tttacagttc gtggagaaac aatggcatgc aagatcaaga tcaggatgtg attcgtgagt
    14221 tcctggtcga gagtcatgaa aacctctcgc gcctggatca ggagttggtt gagttggaaa
    14281 agcgccccaa ggacgcggtg ctgctggccg gcatcttccg caccatccat actatcaagg
    14341 gaacgtgcgg ctttctcgcg ttctcgactc tggaagctat cacccaccag gcagagagcc
    14401 tcctgagcca gttacgcgac gggcaacggg agttgaatcc ctctctggta acgctgatcc
    14461 tggacacggt cgatgcgacg cgcaaagtgc tggcttcgat cgaggccact ggggaggaag
    14521 gtccggatcg ctttggggaa ctgacggaac gtctgcgtgc cgccgcgcaa gcacctgccg
    14581 gcagtaaggg ccagcaggct tccggtaagc ttcctgatcc tgtggagcct cccgacaaca
    14641 acgaccgctt ggagcacccc gaagtcacaa agcccgaaga ggaggcggcg aaatcctcag
    14701 cggtagtgga cgccaacatc cgggtcgggg tggggctgct ggataagctc atggatctgg
    14761 tcggggagtt ggtgttgact cgcaatcaga tactacagtt caacaccgag cgggaggacg
    14821 ccgcactcaa cgcgacctca cagcggctga acctgattac cacggagttg caggaaggcg
    14881 tcatgaagac gcgcatgcag cccatcggca tggtctggaa caaactgccg cgcgtggtgc
    14941 gcgacatggc cgtggctctg ggtaaacaga tccggctgga gatggacggc gccggaacgg
    15001 aactcgaccg gaccatcatc gaagccatca aagacccgct ggtacatttg gttcgcaatg
    15061 cgtgcgacca cggcatcgag ccgccggacg tgcgcgtgcg tgcaggcaaa ccagcccagg
    15121 gcagactgac attgcgtgcc tatcacgaag gcgggcaggt caacatcgaa atcggagacg
    15181 acggagcggg cgtcgacgtg acgcgggtca agcaaaaagc catcgagaaa ggactgctgc
    15241 gacccgaaca ggccgagaaa ttgagtgatc gggaggccgt gaacctgatc ttccaggccg
    15301 ggttctcgac ggcgcaaaca gtcaccaacg tttccggacg cggagtcggc atggatgtgg
    15361 tgaagtccca catcgaaaag atcggcggta ttgtcgacgt gttcagccgg ccgggcgaag
    15421 gctccaccgt taagctcaag attcctctta cgctggccat cattcccggt ctcgtgatta
    15481 cgagtggagg agaacggttc gtcattcccc aggtcagcct gctcgaactg attcgcctgg
    15541 agggggattc caaggaaaag catatcgagc acgtgcatgg gacaccggtg taccgccggc
    15601 gtgggagtct tttgcccatt gcctacctca atgaggtgct cggactgagc tcggctgagc
    15661 gggccgaggc ggtcagcatg gtggttctcc aggctgaaga ccggcaattc gggctggttg
    15721 tggacggcat caacgatacg caagagatcg tagtaaagcc tttgggtaag caattaaagg
    15781 ggctcaccgt ctacgctggt gcgacgatca tgggcgacgg ccgcgtggcc ctcattctgg
    15841 acgtactcgg catcggccag cgctccgggg tcctggcgga atcccgcgag ccggcgcgag
    15901 ccgccgcgga acagaagacg caatcggaaa tcgagcagca acgcttgctc ctgttccgcg
    15961 ccggatcctt tgatcgcctg gcggttccac tttctctcgt cgcaaggctc gaagagtttc
    16021 cccgctcggc catcgagtat gcgggcggat gccaggtggt gcaataccgg aatcgcatcc
    16081 tccctctggt tcctctccgg gcggtactgg aatccggcgc gcgggaccag gaccactcgg
    16141 ccgatccagc gcaagtcatt gtattcaatg acggcgatcg cagtgtgggt gtgatggttg
    16201 accagattct ggatgtggcc gaagaagcgg tgacggtacg tcagaaaacg ggccgcgaag
    16261 gtctcctcgg ctcggcggtc gtgggcaagc gagtcactga tttccttgac ctcaatcacg
    16321 tgatctgcat ggccgccaag aattggttcc agggtgttga tggctccgct gccggcaaga
    16381 acattcttgt cgttgaagcc tcggctttct ttcggggtct gattcggacc aacctggaca
    16441 tggcaggcta ccgggttcgg gaggcagcga atctcgatga ggcgattcga gtgctggagc
    16501 agcaaccggt cgacatcgtc gtagcggcgc tggatctgcc acccagcgga agttccgctc
    16561 tgtctgcggc catgcgccgg aggccggagt ggcgcaagat cccgattttg gaactggccg
    16621 attcgaccga gcaggttcgg acgcgagcca gccagtctgc cgatttccag gactgccaga
    16681 tgaaatttaa ccgggaggcg atgctcgaat cggtggcgcg gcttgcttcc gcgttggctt
    16741 ccgctgacgc gtcaccagtg ctggtgggag aggagaaata accgatggca accgaaccgg
    16801 cgaccatgag atccgaaccg gcgcagaccg gcggacagtt tgcaacattt ttcgtggcgg
    16861 acctgttttt tggtgtggat gtgctccgcg tccaggaggt cttgcgtttc cagcagatga
    16921 cccgcgtgcc ccaggcaccg gaggtcatcg agggactgat caacttgcgc ggccagattg
    16981 tcaccgcaat cgatatgcgg cggcggctcg ggttgccgcc ccgcgccggg gaccaggctc
    17041 cgatgaacat ggtggtccgc accgaagacg gtgccgtcag tctgctggtc gacgagatcg
    17101 gagatgtatt ggatgtggat gccgccactc acgaacggcc tccagagaat ctggatccgg
    17161 ccgcgcgcga gctgattcgg gacgtctaca agttgaaaga ccggctgctc ttaattctcg
    17221 atgcggagcg aacggtggat ctcgctgttg agagagcagc gtagtgcata ggaactgccg
    17281 ccggcaactc gctggccagc gaagagagaa tccaggactt cgcgccgagg gcggcgtgcg
    17341 taacgcaatt gaaatacgaa ggagctaaga caatgagaaa acaagtaaac ggcggccgac
    17401 gcattcgtcc cgatcgaaac ccgtccatca gcacggccgt tttggaccgt ccagtgccga
    17461 gcgcaccgcg gatccagacg aaggatcggc ttggggaaga aatactccga gtgacttccg
    17521 ctcttcagca aggcggcctg tcggagcggg tgcgagccga cctgttcgag ggcggcgacc
    17581 gtatcatcgt tgacggcgtg aatgggatgc tggacgcagt aatcgagccg ctgaatgtag
    17641 ccgtcggctt ccttgcccgg gttaacagag gtgacgaact gcacaagatc actcaggaat
    17701 accagggtga ttttcaagtg ctgaaggaca acatcaacag cgtgatctcg aggttgtacg
    17761 gcttgatcga tgagacccag cgtcggtctc aggctggaat caacggagag gtcgggacgc
    17821 gtgccgatct gagcaactat ccgggtggat gggggaggat catcaagggt ttcaacgacg
    17881 cactggatgc ctttaccggc cctctcactg tagcggcgga gtacgtggac cgcatctcga
    17941 agggcgacat cccgccgaag attacagaca cctacaacgg cgacttcaac gagatcaaga
    18001 ataatttgaa tacctgcgtt gattcgctga atagcttgat cgccgaaatg aagcgcatgt
    18061 cagtcgagca tgaaaagggc gacattgacg tgacgattcc ggctggcaag ttccagggag
    18121 cgtaccaggt gatggcccaa gaggtcaacg acatggttgc gggtcacatc agcgacacga
    18181 ggaaggccgt ggcgtgcgtt gctgagttcg gcaaggggaa cttcgaagcg ccgctcgaga
    18241 agttccccgg caagaaagga ctccttaacg acaccatcga acaggtccgg acaaatctga
    18301 aagcgctgat cgccgacacc gacaccctgg tcaaggctgc cgtcgagggg aagctcggca
    18361 cgcgtgccga cgcatccaaa cacctgggag acttccgcag aatcgttgaa ggtatcaaca
    18421 agaccctcga aatggtggtt gagcccctaa aggttaccgc gcagaacgcg acggccctgg
    18481 catcctcatc cgaggagttg acggcggtca gccaccagat ggcaggcaac gcggaagaga
    18541 ccgccacgca ggccaacgtt gtctcggctg ccagcgagca ggtttcgaat aatgtcagct
    18601 cggtcgcttc ggcctccgag caaatgcagg cctcgatccg cgaaatcgcc aagaacgcta
    18661 acgagtcggc ccgggtggcc aagaatgctg tcagcgtggc gcattcgacc aacacgactg
    18721 taacgaaact tggcgagtcc agccaggaga tcggcaatgt gattaaggtg attacctcta
    18781 tcgcgcagca aaccaatttg ctggccctca atgcaacgat cgaggcggcg cgcgccggtg
    18841 aagccggtaa gggctttgcg gtcgtagcca acgaagtgaa ggaactcgcc aagcagactg
    18901 ccaaggccac tgaggatatc ggccggaaaa tcgaggcgat ccagggggac accaaggggg
    18961 ccgtcaaggc gatcgaggag atcagcacga tcatcaacca ggtcaacgac atctccaaca
    19021 gcatcgcctc ggcggtggaa gaacagacgg tgaccactaa cgagatcggc cgcagcgtaa
    19081 ccgaagcagc caaaggtgtc ggcgacatcg ctaagaatat cggcggtgtg gcggtggcgg
    19141 cgaaaaacac gacccaaggg gccaatgata cgcagaaggc gtcgcaggag ttgagccaga
    19201 tggcggcaca gctccaaacg gtcatttcga agttcacgtt ctgacaggtc aacggactgg
    19261 gggcgcggcc cggcggctgc agggccgcgt cccttcaatc aacaaacgaa ggcaggtgag
    19321 tgatggccaa agcgttagtg gtggatgatt cgaaggccgt tcgaatgatt ctcgcaaaga
    19381 tcttaaggga gctcgggtac gaggttcgcg aagcggagaa cggaagaggc gcccttgagg
    19441 tgatcgaggc cgagaagaat gcagtggcgc tggtcctggc agattggaac atgcccgaga
    19501 tcaatgggtt ggatctgctc aagcgattgc gccagaaccc ggaactttct tcgctcgtgg
    19561 tggttatggt cactaccgaa accgggctgg atcagatgat ggcagccctg gaggcgggcg
    19621 ccaatgagta tgtcatgaag cctttcacaa aagacattct ggtagagaaa ctccagcttg
    19681 cggggatcca tccttgagga gcggggcatg gctgcgataa acaagcgctt gctccaaccc
    19741 ggagaacgga tccgtgtgct ggtggtcgac gactcggtgg tcatccggcg cctggtcact
    19801 cacgcccttg agcagcatcc ctcagtcgag gttgtgggga cggcagcaaa tggggtgatc
    19861 gcgctgcaaa agatcccgca ggtaaatccc gacgtcctca cgttggacat tgagatgccg
    19921 gagatggacg gactggaaac cttgcggcgc attcggcgtg aatatcccca cctgcgggtc
    19981 atcatgttca gcacgctcac cgaacgcggt ggggcagtga ctctggaggc attgagcctc
    20041 ggtgccgacg actacgtggc caaggcttcc aacgaaggat cgctggatcg ctcgatggcg
    20101 cgcctgcgag aagagctggt tccgaaaatc aagcagttct ttcacatacc cgcgctaaat
    20161 agtgcggtgg cccggccggc gccaggacag gctccggtgc cgccgccgat ctggcggagc
    20221 acgaaagtgc ggcctaaagt agtcgtgatc ggcgtttcca cgggggggcc aaccgcgctg
    20281 ggtgagatcc tgccggagtt gccggccggg ttcccgctac cagttcttgt ggtccagcac
    20341 atgccacctc tttttacaag gctgcttgct gagcgtctcc accctatctg ccaactaccc
    20401 gtcgaggaag ggagtcaggg cgcactcgtg gacgctggca gcattctgat tgcaccaggt
    20461 gacttccaca tgaaagtggc ctcaaacggc gacagggtac gtatacatct cgaccagtca
    20521 ccgccacaaa actcctgccg cccagccgtc gatgcgctgt ttacatcgat cggtgaggtt
    20581 tatggtggtg ctgtcatcgc tgtgattctg accggaatgg ggcaggatgg gttgcgcggc
    20641 acgcggattc tcaaagcaca gggcgcgagc gttctggcgc aggatgaggc ttcgagtgtt
    20701 gtatggggaa tgcccggcgc cgtggtgaac gccaatcttg ccgaccgcgt ggtgccgctc
    20761 gatcaagtcg tgccagagat tctgcgcata gtggggcgag cttaagggga gttggatgcc
    20821 tagccaaacg tcaattgcgg agatccgctc ggagaactac cggttcctcc aacagcatgt
    20881 ctattctcat gcgggcatcg tgctggaaga agacaagcac tacctgttcg aaagccgcct
    20941 cgcacccatc gtcaagcaac tcggcctcaa ctcgatcaat gacttgtgta cgttgctcat
    21001 ggctacccgg gagacccaag tgggccggca ggtcgtcgag gccatgacga ccaacgaaac
    21061 ctacttcttt cgcgaccccg cccagtatga tgccatccga acggtcttgt tgcccaggct
    21121 gaggcgggag cgtcagaata tgagaacact gcgcttctgg tcggctgcgg catcgaccgg
    21181 gcaggaagcc tacagtctgg ccatggttct cctggaagac gggttcagcg actggaacat
    21241 tcagattctg ggcactgatc tctcctcccc ggtcttggaa cgggcccggt ccggtaagta
    21301 tcagcagatt gaggtgaacc gcggtttacc tgcggcgctt ctggtaaagt acttccgccg
    21361 ctcgggcatg gactggcact tgagcgaggc g
//
LOCUS       DASTMK010000188         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_585643, whole genome shotgun sequence.
ACCESSION   DASTMK010000188 DASTMK010000000
VERSION     DASTMK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585643"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFQ79_08205"
     CDS             <1..412
                     /locus_tag="VFQ79_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEU0139677.1"
                     /translation="MPAGGSRMPASAETVEKRLHYVHQEGQQVFKYAVRKMYEVCRDL
                     LEKNNLGPNDIAVMIPHQANRRIITACAERLGIDCNKVIINIDRYGNTTSATLPLATR
                     DAIQTGKLKTGDLVLFAAVGAGYTVGANLWRWSY"
     gene            663..3998
                     /locus_tag="VFQ79_08210"
     CDS             663..3998
                     /locus_tag="VFQ79_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0139678.1"
                     /translation="MIVPPPAKSQVLYGSVVGTVEDPTGAVVPGAKVTMTNTSTGLMR
                     EVHADDQGRYTVVSLPAGTYEMVVTASGFRTLARTGIEVTINNVTRVVAQLEVGQVTE
                     QVTVSASAAALQTDRADTRAEISSRTVVELPLPAYRNFQSMINLVPGATPAAFQNSMG
                     ASPQRSLTTNVNGTNRNNNNTRVDGAINVYIWLPHHTLYNPPVESIETVNISTSSFDA
                     EQGMAGGAAVTVATKSGTNTLHGSGYWYHDNQHLYARPYFYQQATPKPQLPKSIVNIP
                     GGTIGGPVVKNKLFYFFSFERTWERTGQFANSSTPPADVRAGDFSAYSGLGNIYDPLT
                     GDANGAGRVPFANNQIPASRFSPTWTKIQQLAPLPNQPAQDAFGLTGNYFSSATLGLT
                     RDQYDIKTNYNVNTQLMIWGKYSQMNAPVTGAGALGELTGPALGQLGKADVRVIIPSA
                     GFNLTLSPTFLIDGVFGHTRFKNDATGPDYGRNWGSEIWGIPGTNGANVFAGDIRYSG
                     QPCIANGFTSWGNCSASNPNFYNDRSYTYTTNFSKIERAHELRWGVDIVRHEMNHWQP
                     EIANPRGNLSPTGNAVVLQGQVARSIHTYAAGLLDILGSANKSVQFFDMKTREWQLGF
                     YFRDRWQVTRNFTVNLGARYELYPMMNRGDGRGIERWDPVTNLVTLGGVGNVPTDNGM
                     SVSKKLIAPRLGIAWRLNNDTVLRSGYGITFNPMVLSRPLRGLYPSTIAASWVAPTQF
                     GYFGTLTQGIPDVPTPDISSGIIELPATVNMGPRSPWGGQLNRGYIQSWNFTIERKLP
                     AEFIVSAGYVGNQTVRQFLDLDINAATYIGGGPSSRPLAATLNRLIETLMWDGWGNAN
                     YHSLQVSLNKNFSHGLFMKGAYTWSKAINMADDDGWQAMPLTNMPDALHRNRAVAGYD
                     RPHMLVMSWVYELPWGQGRSLPLSGILDTVAGGWRVNGIYSAYSGTPFNVTASTASLN
                     TPGSNTQTADQVGEIVKIGDVGPGTQYYDVTAFRDPNFQRPAGTFRFGSMGRHALRGP
                     GFQKVDLALFKDFRLTERFTLQFKAEAFNFTNTPRFGNPAANVSNMTVNASTGAITNV
                     NNFMAITSASDERKFRFGLRLSF"
BASE COUNT          900 a         1342 c         1155 g          813 t
ORIGIN      
        1 catgcccgcg ggcggaagcc gcatgccggc ttcggcggaa accgtggaaa aaaggctgca
       61 ttacgtccac caggaaggcc agcaggtatt caaatacgcg gtacgcaaga tgtacgaggt
      121 ctgccgcgat ctgctcgaga aaaacaacct cggcccgaac gatatcgccg tgatgatccc
      181 gcaccaggca aaccggcgga ttatcaccgc gtgtgccgag cgcctcggaa tcgactgcaa
      241 caaggtcatc atcaacatcg accgctatgg gaacacgaca tcggcgacct tgccgctggc
      301 cacgcgggac gcgatccaga ccggcaagct gaagacaggc gacctggtgc tgtttgcggc
      361 cgtaggcgcg ggttacactg tgggcgccaa cctctggcgt tggagctact gagaagttca
      421 gccaccctca ccccaccatc ccggccctta acgctacctc ccgttagaaa taggaattgc
      481 ttcgtagatt ggcaaatgtt aaggtttgat cgaatcggct acgtctggtt cccatttgcc
      541 tgacggagcc ggaaacgggg agcagtttct ttcccacaga gacgagagga tacgctatga
      601 aaaccagcgc cttgcagttg gaaagaacgt ttgtctgtct attggctatc ttgttgctag
      661 ccttgatagt cccaccgcca gcaaagagtc aggttcttta tggctctgta gtcggcacgg
      721 ttgaagatcc gaccggagcc gtggttcctg gagcgaaagt cacaatgacg aatacctcca
      781 ccggcctgat gcgagaagtt cacgccgatg atcagggccg ttataccgta gtgagtttgc
      841 cggccggcac gtacgaaatg gtagtgacgg cgagcggctt ccgtaccctt gcccgcaccg
      901 gcatcgaagt gaccatcaac aacgtcacac gcgtggtggc gcaactcgag gttggccagg
      961 tgaccgagca ggttaccgtg tcggcgagcg ccgccgccct gcaaacagac cgcgcggata
     1021 cccgcgctga aatcagctcc aggaccgtag tggagcttcc gctgccggcc taccgcaact
     1081 tccaaagtat gatcaacctg gtgccgggcg ccacgccggc ggcgttccaa aactccatgg
     1141 gcgcctcgcc ccaacgttcg ctcaccacga atgtcaacgg caccaatcgc aataacaaca
     1201 acacgcgcgt ggacggagct atcaacgttt acatatggct gccgcatcac acgctgtaca
     1261 atcctcccgt cgagtcgatc gagaccgtca atatttcaac ctcatccttc gatgcggagc
     1321 agggcatggc cggcggggcc gccgtcaccg tggccacaaa gtccggcacc aacacgcttc
     1381 atggttcggg ttattggtat cacgacaacc agcatcttta cgcacgtcca tatttctacc
     1441 agcaagcgac gccgaagccc cagcttccga agtcgatcgt caacattccc ggcggcacca
     1501 tcggtggtcc ggttgtgaag aacaagctgt tctacttttt cagcttcgag cggacgtggg
     1561 aacgtacggg ccagttcgcc aattcctcga caccgccggc ggacgtgcgc gcgggcgact
     1621 tttccgccta ctccggcctg ggaaatatct atgacccgct aacgggcgat gcgaacggtg
     1681 ccggccgtgt gcctttcgcc aataaccaga tccccgcgtc ccgcttctcg ccgacgtgga
     1741 ccaaaattca gcagttggcg ccgctgccca accagccggc acaggatgcc ttcggactta
     1801 cgggtaacta cttcagctcg gccactctcg ggctgacgcg cgatcaatac gacatcaaaa
     1861 ccaactacaa cgtcaacacg cagttgatga tctggggcaa atactcccag atgaacgctc
     1921 ctgtcaccgg cgcgggcgcg ctcggcgagc ttacgggccc cgcgcttggc cagcttggca
     1981 aagccgatgt acgggtcatc atccccagcg ccggtttcaa cctgactctt tcgcccacct
     2041 ttctgatcga tggcgtgttc ggccacacac ggttcaagaa cgatgccacc gggccggatt
     2101 atgggcgcaa ctggggctcc gaaatctggg gcataccggg aacgaacggc gccaatgtat
     2161 tcgccggcga catccgctat tcgggccagc cttgcatcgc aaacggtttc acgagttggg
     2221 gaaattgcag cgcctcaaat cccaacttct acaacgaccg cagctacact tacacgacca
     2281 acttctcgaa gatcgaacgc gcacacgaac ttcgctgggg cgtggacatc gtccgtcacg
     2341 agatgaacca ctggcagcct gagatcgcga acccccgcgg gaatctcagc cccacgggga
     2401 acgccgtggt gttgcaggga caggtggcgc gcagcatcca cacttatgcc gccggtctgc
     2461 tcgatatcct cggcagcgct aacaagtccg tgcaattctt cgatatgaaa actcgcgaat
     2521 ggcagctcgg cttctatttc cgcgatcgct ggcaggtcac acgcaacttc accgttaacc
     2581 tgggcgcacg gtacgagctt tatccgatga tgaaccgcgg cgacggacgc ggtatcgagc
     2641 gttgggaccc ggtgacaaac ctcgtcaccc tgggcggagt tggcaatgta cctaccgaca
     2701 atggaatgtc cgtatcgaaa aagctgatcg ccccgcggct cggcattgca tggcggctga
     2761 acaacgatac cgttctccgc tccggttatg gcatcacatt caatcccatg gtgctctcac
     2821 gcccgctacg cggcctctat ccttccacca ttgccgcgag ttgggtcgcg cccactcagt
     2881 ttgggtactt cggtacgctc acgcagggaa tcccggacgt gccgactccc gacatcagct
     2941 cgggcatcat cgaactcccg gccacagtca acatgggtcc tcgcagccct tggggcgggc
     3001 agttgaatcg cggctatatc cagagttgga acttcaccat cgagcggaaa ctgccggctg
     3061 aattcatcgt gtccgcgggc tatgtgggca atcagaccgt ccgccagttc ctggatctgg
     3121 acatcaacgc cgcgacctac atcggcggcg gcccctcaag ccgcccactt gccgccactt
     3181 tgaaccgtct catcgaaact ctcatgtggg acggttgggg caacgccaac tatcactcgt
     3241 tgcaggtctc actcaataag aatttctcgc acggcctgtt catgaagggc gcctacacat
     3301 ggtcgaaagc catcaacatg gcggacgacg atggctggca ggcaatgccg cttacaaaca
     3361 tgccggacgc gctgcaccgc aaccgcgccg tggcgggcta tgaccgcccg cacatgcttg
     3421 tcatgagctg ggtctacgaa ctgccctggg gccagggccg ctcgctgccg ctctccggaa
     3481 ttcttgacac agttgctggc ggctggcgcg tcaacggtat ttactccgcc tatagcggca
     3541 cgccattcaa cgtgacggcc agtactgcat cgctcaacac ccccggctcc aatacccaga
     3601 ccgccgatca ggttggagaa atcgtaaaga tcggcgatgt tggcccgggg acgcagtatt
     3661 acgacgtcac tgctttccgc gatccgaact tccagcgccc agccggaaca ttccgttttg
     3721 gtagtatggg acgccacgca ctacgcgggc ccggcttcca gaaagtcgac ctcgcattgt
     3781 tcaaggactt ccggctgacg gaacgcttca cgctgcagtt caaggcagag gcgttcaact
     3841 tcacgaacac gccccgcttc ggcaatcccg cggctaacgt cagcaatatg accgtgaatg
     3901 cgtcaacagg cgctatcacc aacgtgaaca acttcatggc gatcacgagc gcgagcgacg
     3961 agcggaaatt ccgcttcggc ctgcgattgt cattctaacg aaccggtgcg gcgccctctt
     4021 aaaaggcgcc gcactcttca atccacgaca tcatacagat tcgcttgata ctgcacgccg
     4081 gccaggaccg cctttatcgc gggccacgcc tgctggtgat cttcgcttgc cacccattgc
     4141 cgtctctgct cctcggtttc gaagcggatc agaagccggt gtgtaaagtc gcctggttcg
     4201 ctcccggagc
//
LOCUS       DASTMK010000189         5513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_392936, whole genome shotgun sequence.
ACCESSION   DASTMK010000189 DASTMK010000000
VERSION     DASTMK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392936"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..927)
                     /locus_tag="VFQ79_08215"
     CDS             complement(10..927)
                     /locus_tag="VFQ79_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0139679.1"
                     /translation="MLSRRDLLHRALPAIAASRMMAADASAQVRFAAISDIQYADQEN
                     AGKRLYRESLVKLENIIGQLRNQSPDFVIHLGDLVDRGAENADRIMPLFSRLPRPQYQ
                     VLGNHDFFGPRNNVLRRFGMKRPYYDFARPGWRFIVLDGMYVSVKGGWPEESPQYLEG
                     KKMLAELQERRAPNARDWNGAVGAEQRSWLRQTLEAASRRGERAIVCCHFPVLAAATT
                     PAHLLWDHEEVLGILDAQPAVAAYLCGHDHNGGYAERNGIHHITMKGVVENDVAECVR
                     MVELHSDRIELRKPGAKEGRVLALARRAG"
     gene            complement(948..3572)
                     /locus_tag="VFQ79_08220"
     CDS             complement(948..3572)
                     /locus_tag="VFQ79_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139680.1"
                     /translation="MNWWRRLLRKEELERQLDSELRFHFEYQVAEFVRNGMSEPEARR
                     RARIEFGGIEQTKEACRQARGTLWVETVVADIRYAARTMRRSPAFAIAAISTLALGIG
                     VNTAIFSTFNTVFLRPLPYVQPERLVWTTKYFPRFNGDRMFVPEYAAWRRQNTAFEHL
                     EAYGIGIGVNLTSENRSAERVRAGHVTPGFFTMLGVQPHLGRTFAPDEDQPGRNHVAI
                     LSNSVWRNFFESDPAVLGKAIALNGKPGIVIGVMPPGFIDPAADDTGIWLPDAVDAAS
                     AVPGRGMRPLSGVIGRLKPGATVESARASLEVAARSMSAQYPAPWSAYHAAARVRVVP
                     LQEQLTHGSKILIFVLMGAVGFILLIVCANLANMFLSRAVVRQKEIALRAAVGASRSR
                     LVRLLLTESLLLGLCGGLAGMVLAYWGASALSFLLPEAIPRHVPIDTRVLGFAAFCSV
                     ASGILFGLAPAVIASKPDLNTSLKEGGRSLARHQRSFRFQRVLVVAQVALSLVLLIGG
                     GLLLRSFLALMDVNPGFNPRKVLLAEVSLAPQELYGPPQQVRFFRRALEAIRNIPGVQ
                     HAAITDESPLATFQSVVSGLAAEGQLPTDDTVVPVAASAGYFNALQIPLLAGRFFNDR
                     DGEGGPRVAIVNQTLARILFLGVDPVGRRIHLGSETEPWVTVIGVVADIRHRGLDDKI
                     WPELYQPYMQAPTPWMTFVIKASADSSGLIPAVRKAIAGIDRNQPLFDIEWMERRLSD
                     SVAQRRQRAALLGSFALLALVIAAVGIYGVMAYSVAQRTHEVGVRMALGAQPGAILRM
                     VVADGMRLAVTGVALGIAGAIGLSRLLSSFLFGVTATDTATFLSVSVLLISAACLAAY
                     IPARRAARADPAFALRQD"
     gene            complement(3569..3916)
                     /locus_tag="VFQ79_08225"
     CDS             complement(3569..3916)
                     /locus_tag="VFQ79_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139681.1"
                     /translation="MHSPKADLPQGTLDLLILKVVAAGPIHGYAIAQRIRQISREVLQ
                     VQQGSLYPALHRLENKKLLSAEWKVSETGREAKFYRLTAQGRAQLKSETRDWTRLTEA
                     VGLILRGAEGEAL"
     gene            4023..5360
                     /locus_tag="VFQ79_08230"
     CDS             4023..5360
                     /locus_tag="VFQ79_08230"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0139682.1"
                     /translation="MNDKVRGNKARSRSGRGWILVAIGLLVVAIGGKWLFISVAGQVL
                     HPNPQAIPSVTHSDPSPEWSGAVDRARLIIRAGLAGQNLPGLSVAVGAGGDIVWAEGF
                     GWADIETYVPVTPDTRFRMGTASTVLTSAAAGVLLEKGLLTLDDEIQTYVPQFPKKQW
                     TVTLRQLMAHVGGVGTDGGDEGPLFRQRCERPVEALPHFAHNGLLFEPGTQYRHSKYG
                     WILVSAAVEAAADQPFLTFLREQIFLPLGMDNTGAESAMEENPERVGEPAEDAPFLTF
                     IRQMILEPLGIGGTKARPATDPATVYFPGFGYDPLVRYGLHVMRPHNLSCYAGSMAFF
                     STPSDLVRFGLAVNGGALLRPATVRLLQTSQQLTSGQETGYGLGWDLDTVTLAGEPTP
                     AAGHDGELLGRRVVSLMLFREAGIVVAVMSNISDADAPALALKVAEAFAQPAR"
     gene            5406..>5513
                     /gene="rpmI"
                     /locus_tag="VFQ79_08235"
     CDS             5406..>5513
                     /gene="rpmI"
                     /locus_tag="VFQ79_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU0139683.1"
                     /translation="MPKLKTHKGASKRFRKTGTGKLVRNHSTARHILTSK"
BASE COUNT         1108 a         1707 c         1651 g         1047 t
ORIGIN      
        1 gcaaagagtt catccggcgc ggcgcgccag cgcaagaacc cggccttcct ttgcacccgg
       61 ttttctgagc tcgatgcgat cggagtggag ttccaccatt cgtacgcatt ccgccacatc
      121 gttttccacc acgcccttca tcgtaatgtg gtgaatgccg ttccgttcgg catagccgcc
      181 gttgtgatcg tgtccacaca agtacgcggc gaccgcgggt tgtgcgtcga ggatgcccag
      241 cacctcttcg tgatcccaca acaggtgtgc tggtgtggtg gctgccgcca acaccggaaa
      301 gtggcagcag acaatcgccc gttcgccgcg ccggcttgcg gcttccaggg tttgccgcag
      361 ccaactccgc tgttctgctc ctactgcacc gttccaatcc ctggcgttgg gcgcgcgccg
      421 ctcctgtaac tccgcaagca tttttttgcc ttccagatac tgggggcttt cttccggcca
      481 ccctcctttg acgctcacat acattccatc caacacgatg aagcgccatc cagggcgagc
      541 aaaatcgtaa taggggcgct tcattccaaa gcggcgcagc acgttattgc gaggcccaaa
      601 aaagtcgtga ttgccgagaa cttgatactg cgggcgaggg aggcgactga agaggggcat
      661 gatacggtcg gcgttctctg caccgcggtc caccaggtcg cccagatgga tgacaaaatc
      721 cgggctctga ttgcgaagct gcccgatgat gttctcaagc ttcaccagcg attcgcgata
      781 gagccgcttg cctgcgtttt cctgatcggc gtactggata tccgagatgg cggcgaacct
      841 tacttgtgcg gacgcatcgg ctgccatcat tcgcgaagcg gcaatcgcgg gaagcgcccg
      901 gtgcaggaga tctcgtctgc taagcatcga tagcagtcta tcgcgaatca atcctgccgc
      961 aacgcaaatg ccgggtctgc tcgtgctgcc ctccgcgctg gaatatacgc ggcaagacag
     1021 gcggccgaga tcagcagtac agagaccgaa agaaaagtcg cggtgtccgt cgctgtcact
     1081 ccgaaaagga aactcgaaag caagcgcgac aacccgatcg ctccggctat gccgagagcc
     1141 actccggtca ccgccaggcg cattccatcc gccaccacca ttcggagaat ggccccgggt
     1201 tgggcgccca gagccatccg gacgcccact tcgtgcgtcc gctgcgctac tgagtaggcc
     1261 attacaccat agatgccgac ggccgcgatc acaagcgcaa gcagcgcgaa ggaaccgagc
     1321 aaggctgccc tttgccgccg ctgtgccacc gaatccgaaa gccgccgttc catccattcg
     1381 atatcgaaca atggttggtt gcgatcgata ccggcaatcg ctttccggac cgccggaatc
     1441 aaccccgaag agtctgcgga cgctttgatg acaaaagtca tccagggagt gggagcctgc
     1501 atgtacggct gatagagttc gggccagatc ttgtcgtcca ggccgcggtg acggatatcc
     1561 gccaccactc ctatcaccgt gacccacggc tccgtttcgc ttcccaaatg gatgcgccgt
     1621 cccaccgggt ctacacccaa aaacagaatg cgggcgaggg tctggttgac gatggctact
     1681 ctcgggcctc cctctccatc gcggtcatta aaaaaacggc ctgcaagaag cggaatctga
     1741 agagcgttga agtagccggc gcttgcggcg acgggaacaa cggtgtcgtc cgtgggcagc
     1801 tgtccttccg cagccaagcc gctcaccacg gactggaacg ttgcgagagg actttcatcg
     1861 gttatggccg catgttgtac gccggggata tttcgaatag cctccaatgc acgacggaag
     1921 aaccgtacct gctgaggcgg cccgtaaagc tcctggggag ccagcgaaac ctccgccagc
     1981 agaaccttgc gcggattgaa accgggattc acatccatga gcgcaaggaa gcttctgagc
     2041 agcaatcctc ctccgatcaa caacaccagc gacagcgcga cctgcgctac aaccagaaca
     2101 cgctggaagc gaaagctgcg ctggtggcgc gccaggctcc ggccgccctc cttcagactg
     2161 gtgttcagat ccggcttgga agcgataacg gccggcgcca ggccgaacag aataccgctt
     2221 gccaccgagc agaacgcggc gaaaccaagc acacgcgtat cgatcggaac gtgccgggga
     2281 attgcttccg gcagcaggaa gctcaaggcg gaagcgcccc aatacgccag caccattccg
     2341 gccagtccgc cgcaaagccc caacagcaga ctctccgtca gaagcaatcg caccaggcgg
     2401 gagcgggagg cgcccacagc ggcccgcaac gcgatttcct tttggcggac gaccgcgcgg
     2461 gaaagaaaca tgttcgcaag gttggcgcac acaatcagca gaatgaatcc caccgctccc
     2521 atcagaacga aaatcaaaat tttggagccg tgggttaact gctcctgaag cggcactacc
     2581 cgtacgcgtg cggcagcgtg ataagcagac cacggggcgg ggtactgggc actcatgctc
     2641 ctggccgcca cttcgaggct ggcgcgagcg ctctcgacgg tcgcacctgg tttcaaccgc
     2701 ccgatcacac cgctgagagg ccgcatgccg cgtcccggta ccgcactcgc ggcatccacg
     2761 gcgtccggca gccagatccc ggtatcgtcc gcggcaggat cgatgaaccc gggaggcatg
     2821 actccgatga cgatgccggg tttaccattg agcgcgattg ctttgcctaa aaccgccgga
     2881 tcagactcga aaaaattccg ccaaacggag ttgctcaaga tggcgacgtg attccggccc
     2941 ggctggtcct cgtccggtgc aaaagtccgt ccgaggtgcg gttgtactcc cagcatcgtg
     3001 aagaatcccg gtgtgacgtg accggcgcgc acgcgttcgg cggatcgatt ttcgctggtg
     3061 agattcactc caatgccgat tccgtatgct tccagatgtt cgaaagccgt gttttgccgt
     3121 ctccaggcgg catattccgg aacgaacatc cgatcgccgt tgaacctggg gaaatatttc
     3181 gtcgtccaaa ccaggcgctc gggctgaaca tacggcagcg gccgcaggaa taccgtgttg
     3241 aaggtgctga aaatggccgt attgaccccg attcccaaag ccagcgtgct gatggcggcg
     3301 atggcaaagg cggggctcct tctcatcgtg cgggccgcgt accggatatc cgccactacg
     3361 gtctccaccc atagagtgcc tcgcgcctga cggcatgcct ccttggtctg ttcgattcct
     3421 ccaaattcga tgcgtgcgcg ccgccgtgcc tccggttcgc tcattccgtt ccggacgaac
     3481 tcggcaacct ggtactcgaa atgaaaccgc agttcactgt cgagttgtct ttccaactct
     3541 tctttcctca ggaggcgcct ccaccagttc acagagcctc tccttcagca cctcgaagaa
     3601 tcagcccgac ggcttcggtc agccgggtcc agtcccgggt ctcactcttc agttgggcac
     3661 ggccctgagc cgtgaggcga tagaacttgg cttcccgtcc ggtttctgac actttccatt
     3721 ccgcggacag gagctttttg ttctccagcc ggtgtaacgc agggtagagc gatccctgtt
     3781 gtacctgcaa gacttcccgg gagatttgcc ggattctctg cgcgatcgca tatccgtgaa
     3841 tcggccccgc ggcgaccacc ttgagaatca gaaggtccag agtgccttgc ggcaaatcgg
     3901 ctttcggcga gtgcatcatt tgcgtttacc tggcgcttca acacatatca cacctacatg
     3961 tagaagttca aggtgtgtat gtcttgacat acataatact gttatgcata attctgttag
     4021 gcatgaacga taaagtaaga gggaacaagg cgcggagccg ctccgggcgt ggttggattc
     4081 tcgtagctat cggcctgctg gtggtagcga ttggggggaa gtggctattt ataagcgtcg
     4141 cggggcaggt cctgcacccg aacccgcaag ctataccctc ggtgacgcat tcggacccat
     4201 cgcccgaatg gtccggggcg gtggaccgcg cacggctcat catacgggct ggcctcgccg
     4261 ggcagaacct gccggggctg tcggtagccg ttggcgccgg cggcgacatc gtgtgggccg
     4321 aaggctttgg ctgggcggat atcgagacgt acgtgcctgt cacgccggat acccggttca
     4381 ggatgggaac agcctccacc gtactcacgt ccgccgcggc tggcgtgctg ctggagaagg
     4441 gcctgctaac actggacgac gagattcaga cctacgtccc gcagtttccg aagaagcagt
     4501 ggacagtgac actgcggcag ttgatggcgc atgtgggcgg tgttgggacc gatggcgggg
     4561 acgaggggcc gctgttccgt caacgctgcg agcggcctgt tgaagcgctg ccgcacttcg
     4621 cgcacaatgg gctgctgttc gagccgggga cgcagtacag gcattcgaaa tacggctgga
     4681 tcctggtgag cgcggcggtg gaagccgcgg ccgaccagcc gttcctcacg ttcctgcgcg
     4741 agcagatctt cctgccgctg gggatggaca acaccggcgc cgagtccgct atggaggaga
     4801 atccggaacg ggtgggcgag ccggccgagg acgcgccgtt cctcacgttc atccggcaga
     4861 tgatcctgga gccgctgggg atcggcggca cgaaggccag gcccgcgacg gatcccgcga
     4921 cggtctactt cccagggttc ggttacgacc cactcgtccg ctacggtttg cacgtgatgc
     4981 gtccacacaa cctgtcctgc tatgcgggat ccatggcatt cttctccacc ccgtccgact
     5041 tagtgcgctt cggcctggcg gtcaacggcg gcgcgctgct gcggccggcc acggtgcgat
     5101 tgctccagac gtcacagcaa ctgacgtccg ggcaggagac ggggtacggt cttggctggg
     5161 acctcgacac cgtcacgctg gccggtgaac ccacgccggc ggccggccac gacggcgagt
     5221 tgctgggccg gcgagtggtg tcgttgatgt tgtttcgcga agccgggatc gtcgtggccg
     5281 tgatgtcgaa catttccgac gcggatgcgc ccgctcttgc gctgaaagtc gcggaggcct
     5341 tcgcgcagcc cgcgcgttga tgaaacggca ggaaagtcta taaacacccc gcatcaggac
     5401 caagaatgcc taagctgaaa acacacaagg gtgcgtctaa acgtttccgc aagacgggga
     5461 caggaaagct ggttcgcaat cattcgactg cccggcacat cctgacttcg aag
//
LOCUS       DASTMK010000190        11912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_361556, whole genome shotgun sequence.
ACCESSION   DASTMK010000190 DASTMK010000000
VERSION     DASTMK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11912
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361556"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFQ79_08240"
     CDS             <1..809
                     /locus_tag="VFQ79_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEU0139684.1"
                     /translation="TFTLARLGVPAGLIGPAGNDPAGERMIELLKDAGADTSRIERLE
                     EQTATTIALVRSDGARAFLHRPGVSSVAFENPVSLDGISEEYSHFHLANVFALPKLRR
                     TAEETLRNALDQGMTTSLDTGWDAKGEWLNVAGPLLPHLDVLFVNEDEARHLSGASEP
                     EIAARFFLDRGTAAVVVKLGERGCYAASADFNFHAPSFEVNAIDTTGAGDCFTAGFLA
                     SLHYGAEPEDAARFANAVGALNVQSLGSVAGIRSYKETLEWMNSASTQRS"
     gene            851..1264
                     /locus_tag="VFQ79_08245"
     CDS             851..1264
                     /locus_tag="VFQ79_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139685.1"
                     /translation="MLRRRVLGFLTAVPLALAKAADPQSLRGKLVQLPDGTPVIESAG
                     GKRTFPSGDPDTNGVLKDKRLANMDVELLGHFEKPEHFEVDPIHTRSIFVHQNGRRLV
                     VTYWCDVCAIRTYTPGICWCCQKETDLDLREPDAN"
     gene            1264..2337
                     /locus_tag="VFQ79_08250"
     CDS             1264..2337
                     /locus_tag="VFQ79_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEU0139686.1"
                     /translation="MNGSCRHAFLIALLMTSLSPAQNYSAERVTVNSVEIVRLRDVAR
                     DVQVSIAPSLGNNAYEMKVRGKNILWFPYDSPAGLKSKPVQVGNPFLAPWANRIDQEA
                     YWVNGRKYLLNPHLENFRYDHKKQPIHGLLVFAAWSVVDLKAGEDVAEVTSRLEFWRH
                     PEWMAQFPFAHTIEMTYRLTSGVLEVETRVANESEQPMPLVIGFHTYYQLPGVPRDQW
                     RAHVPARESAVLSDALVPTGETRKVSFQNPVNLATTPLDDIFLDLGRNAQGRAEFRVD
                     DGAGSGISVEFGPKYTVGIVYAPPGRDFICFEPMTGITNGFNLAHKGVHKDLLQTVPA
                     RGTWKESFWIRPLADARGSDSEP"
     gene            complement(2371..3174)
                     /locus_tag="VFQ79_08255"
     CDS             complement(2371..3174)
                     /locus_tag="VFQ79_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139687.1"
                     /translation="MAFKRIAAIVLRYFYLVRGSFARFLPLFAWVAVDIVLWGFISRY
                     LNTVAAPGFNFVPALLGAVLLWDFFIRVMQGVTTAFLEDVWSRNFLNIFATPLSIWEY
                     LAGLVVSSTATSSIGLAVMLAMASAIFGLPLLIYGWLAVPFLLVLFLFGIALGIVGAA
                     IVLRLGPAAEWFVWPIPALVSPFAGVFYPISTLPEWMQSIARLLPPSYVFESLRSILS
                     GGAPPLPALFPAGGLVLLDIALACWIFVRVYRHAVRTGLIARYSAESIT"
     gene            complement(3180..3917)
                     /locus_tag="VFQ79_08260"
     CDS             complement(3180..3917)
                     /locus_tag="VFQ79_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139688.1"
                     /translation="MVLSVTALSKSYGDVAAVDRISFHVGRNEIVGLLGPNGAGKTTT
                     INMILGVLEPSGGSIEIQGLNLARRRRQALARTNFAAVYAPLPGNLTVYQNLRVFGLI
                     YGISDAAGRIEALLEEFDLQRFRNVRCGVLSSGEQTRAALAKAMLNNPDLLLLDEPTA
                     SLDPSTAREVRRKIRAVATHGKGGALWTSHNMYEVEEVCDRVLFLSRGRILLEGDPKT
                     LPREHGKENLEELFITVAREPLSLEER"
     gene            3980..5227
                     /locus_tag="VFQ79_08265"
     CDS             3980..5227
                     /locus_tag="VFQ79_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEU0139689.1"
                     /translation="MQNGPFLRRREALSLLGWLPAAASAATAGTGAPPDQPVDMRPVP
                     ADFGSLYASMERLAARRDYSHSFLGNRFRSVKEYRTEGRKILLNAFGYTPPRINPKAE
                     VVDRRDLGEFIREKIVFSTTPDFRIAAYLHLPSNAKGRVPAIVDLHSHGGMFLFGKEK
                     VIDFGTNHPAMVKYHKENYAGRPTATALARRGYAVITIDAFPFGERRLMMAEDAGYGW
                     ERSRYSVQDVEHLNAKCRRKESTIVKSLMYAGMTWPGIVAWDDMRTVDYLVTRPEIDP
                     DRIGCVGVSLGGYRSLLLSGLDDRIRAGCVAGFMSTVRPMIQRHMDTHSFVHFIPAVH
                     ANLDFPDVVALRTPKPLMVLQCSRDGLFPPAGMKDSVAKIAAIYKKAGAEDAFTSRFY
                     DVPHIFNLAMQDDAFAWFDRHLK"
     gene            5519..7243
                     /locus_tag="VFQ79_08270"
     CDS             5519..7243
                     /locus_tag="VFQ79_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU0139690.1"
                     /translation="MAGNQDEGRTDPTVRPEEPNYEQLIDDYSHFSPPSEGEILQGNV
                     LKVTENEVIVDFGYKSEGLVPIEQFRAPDGSINVQTGDVIDVMVDRSGEVEGYILLSH
                     DKATRIRAWDNLEKAYREGLLVAGRVLGRIKGGLSVDVGVQAFMPGSQADTRPLHNLD
                     ALIGQDIPVKIVKLNRRRGNVVVSRKLAMEEELRTRKTEALDHLYEGAIVTGTVKNLA
                     DYGAFIDLGGIDGLLHVSDMSYGRVGHPSEILNVNDEITVRILKFDRDKERISLGLKQ
                     LVPDPWEDIESRYPLESRVIGRVVSITDYGAFVELEPGVEGLIHISEMTWSRRMKHPS
                     RVVKVGDQVEAVVLEVKPKERRISLGIKQLEADPWTTVADRYSIGSLVEGRVRKLTDF
                     GAFIEIEEGVDGLVHISDLSWVKRVKHPSEILKKGQLVQAVILNIDASNRRLSLGVKQ
                     LQPDAWETFFRSHQVGDIVRGVVCRSANFGVFVELSPGVEGLCHNSETPGAADRRKDE
                     PPVPIGQEFDFKIIKMNEAEKKIGLSIRAVADDEERVRLEDYQRQAAAATMTIEEVMN
                     LKEQGEGR"
     gene            7303..7653
                     /locus_tag="VFQ79_08275"
     CDS             7303..7653
                     /locus_tag="VFQ79_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU0139691.1"
                     /translation="MTKADLIEEVSRVVEMTRKDSEVIVEAIFDSIVRSLRTGDKIEI
                     RGFGSFRTRQRQPRIGRNPKTGARVEVPSKRIPYFKPSKELKDLVNESAAAEAGNAEA
                     PPPPAPAEETPHGM"
     gene            7665..8150
                     /locus_tag="VFQ79_08280"
     CDS             7665..8150
                     /locus_tag="VFQ79_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU0139692.1"
                     /translation="MDRLWSPWRFNYVSKAEIPRGCLFCDKPAEQDDRKNLIVYRGDR
                     TFVILNLFPYTAGHLLIAPYAHIATLQDASDEIATEMIRLARLAEKHLRTIYKPEGLN
                     MGINLGECAGAGVAGHLHMHVLPRWCGDANFMTTVGETRVLPEDINVTFEKLAGAFRG
                     S"
     gene            8174..8968
                     /locus_tag="VFQ79_08285"
     CDS             8174..8968
                     /locus_tag="VFQ79_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEU0139693.1"
                     /translation="MKQANLFAELAAEFAGTTVLILFGNGVVAMVLLFGTGVPGEVVK
                     GGFTNITIAWGLGVTMGIYVAGRISGAHLNPAVTLALALFRGFPWRKVVPYSIAQTAG
                     AFSAAALVFWNYRPAFERVDPNLERTAAIFTTFPAFGDFPAAGFLDQVIGTALLLFLI
                     FALTDENNQQGAADLMPLLIGLIVVVIGMSFGGMHGYAINPARDFGPRVFTVLAGFRN
                     NGLTDGTWAFWIPIAGPMLGGALGAAAWDLGIGRCLPSAVRDRVER"
     gene            9043..9549
                     /locus_tag="VFQ79_08290"
     CDS             9043..9549
                     /locus_tag="VFQ79_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU0139694.1"
                     /translation="MEARKVRFDVTFPPGEIEFHDRKLRQITPLQVEGSAELMGVTQE
                     IRVRGRLSVGMEADCDRCLEPAPFPLESEFDLFYRPSEETAGDEKLLDEDESEIGFYE
                     EGGLDLKDIMREQILLSLPMQRICDEACKGICPVCGGNRNISVCGCHEKKLDDRWAAL
                     RELKQQKP"
     gene            9575..9757
                     /gene="rpmF"
                     /locus_tag="VFQ79_08295"
     CDS             9575..9757
                     /gene="rpmF"
                     /locus_tag="VFQ79_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU0139695.1"
                     /translation="MPNPKRRHSKRRTSTRRAHDSLRVSSHAECPNCHEPKLPHRVCP
                     HCGKYKGREIIEVAEE"
     gene            9782..10801
                     /gene="plsX"
                     /locus_tag="VFQ79_08300"
     CDS             9782..10801
                     /gene="plsX"
                     /locus_tag="VFQ79_08300"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU0139696.1"
                     /translation="MVTIAVDAMGGDHAPKTEVEGAIRAARSLGIRVILVGQQDLLQR
                     ELAQHDGVNHLSVEIHHASERVTMDDSAARAVRTKRDSSIRIASRLVREGIADGVVSA
                     GNTGAIMATVKMVQGMIPGVDRPALASAFPTLKGTPAVMVDVGANVDCSPQMLAQFAV
                     MGEIYSRVIFRTESPRVGLLSIGEEEHKGNDLTRSATPLLKTLRLNFIGNVEGRDVYT
                     GTADVIVCDGFIGNVALKVSEGLVDVIKHMLQESLEATITRKIGYVLSKAGFADFKKR
                     VDYSEYGGAPLLGVKGVCIICHGRSNANAIKNAIRVASEFASGNVNRRIEQELSRWQA
                     AARAD"
     gene            10803..>11912
                     /locus_tag="VFQ79_08305"
     CDS             10803..>11912
                     /locus_tag="VFQ79_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU0139697.1"
                     /translation="MKLSRAIATPLVLAFLAAPGFAQKLNPVQWSLTLEPSSAPPGGQ
                     VAVRLTAAMDAGWHLYSLTTPRPPIATTISLAENPAIAGFKVYQPAPVRKLDPNFNTE
                     TETFDGKAVFLIVATIAGDAAGGPVELAAGIRYQACDDKQCLPPRRNTVTATLTIDPA
                     AQPAQIAIPAGYSEPSSGAPSSAQTPAPPPPAAGGEPQPFGTFLLVAFGLGLAAIFTP
                     CVFPMIPITMSFFLKKQAATRRESIVQAGVFALGIIVLFSGLGLLTTAILGPFGVVQL
                     GSNPWVNTFIAAVFLVFGLSLLGAFEITIPSSVLTRLDQASQRGGMLGTLLMGLTFSL
                     TSFACVGPFVGTLLAASVQSGGIQPLLGMAAFATGL"
BASE COUNT         2492 a         3518 c         3551 g         2351 t
ORIGIN      
        1 ccaccttcac tctcgcacgg ctcggggtac ccgccggtct catcggacct gccggaaacg
       61 acccggccgg agagcggatg atcgagttgc ttaaagatgc cggggccgat acctcccgca
      121 tcgagcgcct ggaagaacag accgccacca ccatcgctct ggtgcgttcc gatggagcgc
      181 gagccttcct gcaccggccc ggtgtcagtt ccgtggcttt cgaaaatcct gtttcgctcg
      241 acgggatttc tgaagagtac tcgcatttcc acctggcgaa cgtttttgcg cttcccaagc
      301 tccgccgcac agcggaggag acgcttcgca acgcattgga ccagggcatg acgacatcgc
      361 tcgataccgg ctgggatgca aagggcgagt ggctcaacgt ggccggcccg ctgttgccgc
      421 acctggacgt tttgttcgtg aacgaggacg aggcccgcca tttgagcgga gcttcggagc
      481 cggagatcgc cgcccggttc ttcctcgacc gtggcacggc tgccgtcgtc gtcaaactcg
      541 gggagcgtgg atgttacgcg gcgtcggccg attttaactt tcacgcaccc tcattcgagg
      601 tcaatgcaat cgacacgacc ggcgcgggag attgctttac agcaggcttc cttgcgtctc
      661 tgcactatgg ggcggagccg gaagacgccg cccggtttgc gaacgccgtg ggagctttga
      721 atgttcaatc gttgggatcg gttgccggaa tccggtcgta caaagagaca ctcgagtgga
      781 tgaactcggc ctccacccag cgttcctgat ctcacgctcc cggacccggc ctcgttagaa
      841 tggaattgtt gtgttgaggc gccgcgtgct gggatttttg accgctgtgc cgctggcttt
      901 ggccaaagcg gccgatcctc aatcgcttcg tggaaagctt gtacagcttc ccgatggcac
      961 gcccgttatc gagtccgccg gaggcaaacg caccttcccc tcgggcgatc ccgataccaa
     1021 cggggttctc aaagacaagc gcctggccaa catggatgtg gaactgctcg ggcatttcga
     1081 aaagccggag catttcgaag tggacccgat tcatacccgc tccatcttcg ttcatcagaa
     1141 tgggcgccgc ctcgtcgtga catactggtg cgatgtttgc gcgatccgga cgtatacgcc
     1201 cggcatctgc tggtgctgcc agaaggaaac ggacctggat ctgcgcgaac cggatgcaaa
     1261 ctgatgaatg gctcctgccg gcacgctttc ctgatcgctc ttctcatgac ttctctttcc
     1321 ccagcccaga actattccgc cgaacgggtg acggtcaatt cggtcgagat tgtacgcttg
     1381 cgcgacgtgg ctcgtgatgt tcaggtctcc atcgccccct cgctgggaaa caacgcctac
     1441 gaaatgaagg tgcgggggaa gaacattctc tggttccctt atgacagtcc cgccggactc
     1501 aaatcgaaac cggttcaggt aggcaacccg tttctcgcac cgtgggccaa ccgcatcgac
     1561 caggaggcat actgggtcaa cggcaggaaa taccttctca acccccatct tgagaacttc
     1621 cgttacgatc acaagaaaca gccgattcac ggactgctgg tgttcgcggc ttggagtgtg
     1681 gttgatctga aggccggcga agacgtggcc gaagtcacca gcaggctcga attctggcgt
     1741 catcccgagt ggatggccca gtttcccttc gcgcatacca tcgagatgac gtaccggctt
     1801 accagcggag ttctcgaagt ggagacccgt gttgcgaacg agtccgagca accgatgcca
     1861 ctggtcatcg gcttccatac ctattaccaa ctgcctggtg tgccgcgcga ccagtggaga
     1921 gcgcacgtac ccgcgcgcga atcggcggtg ctctccgatg cactggtgcc caccggcgaa
     1981 acacgcaagg tctcgtttca gaatccggtg aaccttgcca ccacgccgct cgacgatatt
     2041 tttcttgatc tcggccggaa tgcgcaggga cgcgccgaat ttcgcgtgga cgacggcgcc
     2101 ggatcgggga tctcggtgga gttcggcccg aaatatacgg ttggcattgt ttatgcgccg
     2161 cccggacgcg acttcatctg cttcgagccg atgacaggca tcacaaatgg cttcaatctg
     2221 gcgcacaagg gcgtgcacaa agatctgtta cagactgtgc ccgcgcgcgg cacttggaaa
     2281 gaaagcttct ggatccggcc gctcgctgac gcgcgcggct ccgattcgga gccgtgaccg
     2341 tgagggagcg gcctcgttgg aaaccatgta tcaagtaatg ctttcggcgc tgtagcgcgc
     2401 gatcaggccc gtacgcaccg catgccggta cacgcggaca aaaatccaac atgccagcgc
     2461 gatatcgagc aacaccaatc cgccagccgg gaacagcgcc ggaagcggtg gcgcgccacc
     2521 ggagaggatc gaccgcaggc tctcgaatac atacgaggga ggcagcaggc gtgcgatgga
     2581 ttgcatccat tcgggaagcg tggaaatagg atagaagacc ccggcaaatg gcgaaaccag
     2641 cgcgggaatg ggccatacaa accattccgc cgccggtccc agccgcaata cgatcgccgc
     2701 acccacgatg ccgagcgcga taccgaacag aaacaggacc agcaggaacg gcacagccag
     2761 ccaaccatag atgaggagcg gcagaccgaa gatggcactc gccatcgcga gcatcacggc
     2821 cagcccgatg gaactggtag cggtgctgga caccaccagc cctgccaggt actcccatat
     2881 agagagaggc gttgcgaaaa tattgagaaa gttccgtgac cagacatctt cgaggaacgc
     2941 tgttgtgaca ccctgcatca cacgaataaa gaagtcccac aacaatacgg ctcccagcag
     3001 ggccgggaca aaattgaagc ctggtgcggc cacggtattc aggtacctgc tgataaatcc
     3061 ccagaggaca atatcgacgg ccacccatgc aaacaatgga aggaagcgcg cgaaactgcc
     3121 gcgcacaaga tagaagtagc gcaggacaat ggcggcaatg cgtttgaagg ccatcttgct
     3181 cagcgttcct caagcgagag cggttcgcgt gcaacggtga tgaacaactc ttcgagattc
     3241 tctttgccgt gctcgcgcgg cagcgtcttg ggatcgcctt ccagcaggat cctgccgcgc
     3301 gacagaaaca gcacgcggtc gcagacctcc tccacttcgt acatgttgtg cgaggtccac
     3361 agcgctccac ctttgccgtg tgtggcgacc gcgcggattt tccgccgcac ctcccgcgcc
     3421 gtagatggat cgagggatgc cgtcggctca tcgagcagca gcaggtccgg gttgttgagc
     3481 atggctttgg ccagcgcggc gcgcgtctgt tcgccggaag agagcacgcc acatcgcaca
     3541 ttccggaatc gttgaaggtc aaactcctcg agcagcgctt cgattcgccc cgccgcatcg
     3601 ctgatgccgt agataagtcc gaagacacgc aggttctggt acacggtaag gttgccgggc
     3661 agaggcgcat atacagccgc gaaattggta cgcgccaatg cctgccggcg ccgccgcgca
     3721 agattcaggc cctggatctc gatactccct ccgctgggtt cgagcacccc gaggatcatg
     3781 ttaatggtgg tcgtcttccc cgcgccattg ggacccaaca gcccgacgat ctcattgcgt
     3841 ccgacatgga atgaaatgcg gtccacggcc gcaacatcgc cgtagctctt gctcagggcg
     3901 gtgacggaga gcaccataat ttgagggtac cctgcggcgg ctactgcacc ggaacagcca
     3961 atctcgatac gctaggatca tgcaaaacgg acccttcctg cgacggcgtg aagcgctctc
     4021 tttactggga tggcttcccg ccgcggcctc cgcggccacc gccgggaccg gtgcgccacc
     4081 cgaccaaccg gtggacatgc gccctgtccc ggccgatttc ggttcgcttt acgcaagtat
     4141 ggaacggctc gccgcccgcc gcgactactc gcattccttt ctcggtaatc gttttcgatc
     4201 ggtaaaagag tatcggacag agggccgcaa aatcctgttg aatgcgtttg gctacacacc
     4261 gccgcgcatc aatcccaagg cggaggtggt ggaccgccgc gacctgggcg aattcatccg
     4321 cgaaaagatc gtcttttcga ccacgcccga tttccgcatc gcagcctacc ttcacctccc
     4381 aagcaacgcc aaaggccgtg ttccagctat tgtcgatctg cattcgcatg gcggcatgtt
     4441 cctgttcggt aaggagaagg tgatcgattt cggcacgaac catcccgcca tggtcaagta
     4501 ccataaggag aactatgccg gcaggccgac cgcaaccgcg ctcgcgcgcc gcggctatgc
     4561 ggtgatcacg atcgacgcct tcccgttcgg cgagcggcgg ctgatgatgg ccgaagacgc
     4621 cggctatggc tgggagcgct cccgctatag tgttcaggac gttgaacacc tgaatgcgaa
     4681 gtgccggcgg aaagaatcga cgatcgtcaa gtcgctgatg tatgcgggaa tgacctggcc
     4741 gggcattgtt gcctgggacg acatgcgaac cgtggattat ctggtgacgc gtcccgagat
     4801 cgacccggac agaatcggtt gcgtgggtgt ttctcttggc gggtaccgct cgttgctgct
     4861 gtcggggctg gatgaccgca tccgggccgg atgcgtggcc ggcttcatgt caacagtacg
     4921 cccgatgatc caacgccata tggacacgca ttccttcgtt cacttcatcc cggcagtaca
     4981 cgcaaacctg gattttcccg acgtggtggc attgcgcacg cccaaaccct tgatggtgct
     5041 gcaatgtagc cgcgacgggc ttttcccgcc tgccggcatg aaagactcgg tggcgaaaat
     5101 cgcggccatt tataagaaag ccggagcgga agacgctttc acttcgcgtt tctacgacgt
     5161 gccgcatatt tttaatctcg caatgcagga tgatgcgttc gcctggttcg accgccacct
     5221 gaagtgacgc ggaagggttg gggcgccgcg tttagtaccc ataactccgc ccgctgcgga
     5281 cgcacccccc tgcggttgac atcgctaccg gtgtccgcta tattgctgaa gtactgatct
     5341 atcaatggct tgtagtattt ggtggtgaga aattcaggcg cagaccagga actgaaggtt
     5401 atgtctgccg catggtggtg cctggagcgc ttctatcagg ttgacatacg tggtaccgca
     5461 tccggcgcag tcaccagctt cccaggcctt gaacgatcga cgggtcggag aattggctat
     5521 ggccggtaat caagatgagg ggcgtacgga tcctaccgtt cggcccgagg aaccaaacta
     5581 cgaacaactc atcgatgact acagtcattt ctcgccaccc tccgaaggcg agattttgca
     5641 gggtaacgtc ctgaaagtaa ccgaaaacga agtgatcgtc gatttcggat acaaatcaga
     5701 aggactcgta cccatcgagc agtttcgcgc cccggatggc agtataaacg ttcagacagg
     5761 cgacgtcatc gacgtcatgg tagaccgcag cggcgaagtg gaaggttaca ttctgctttc
     5821 gcacgacaag gccactcgca tccgcgcctg ggacaatctg gaaaaagcct atcgcgaagg
     5881 gctgctcgtg gccggacgcg ttctgggccg catcaaaggc ggcctttcgg tggatgtcgg
     5941 ggttcaggcg ttcatgcctg gatcgcaggc agacacccgc cctttgcata acctggatgc
     6001 tctcatcggc caggatattc cggtaaagat cgtcaagctc aaccgccgcc ggggcaacgt
     6061 cgtggtctcg cgaaagctgg ccatggagga agaactgcgc acccgcaaga cggaggcgct
     6121 ggaccatctt tatgagggcg ccatcgtcac gggaacggtc aaaaacctgg ccgattacgg
     6181 tgctttcatt gatttgggcg gcatcgacgg gctcctccac gtgagcgaca tgtcgtacgg
     6241 ccgtgtgggt catccctcgg aaatcctgaa tgtgaacgat gagatcaccg tcaggatcct
     6301 gaagttcgac cgcgacaaag agcgcatatc gctcggattg aagcagttgg tccccgatcc
     6361 ctgggaagat atcgagagcc ggtatcctct cgaatcacgc gtgatcggcc gtgtggtaag
     6421 tattaccgac tacggcgctt ttgtcgagct cgaacccggc gtcgaaggcc tgattcacat
     6481 ctccgaaatg acgtggtcgc ggcgaatgaa gcacccgagc cgggttgtga aggtaggcga
     6541 ccaggtggaa gcggtcgtgc tggaggtaaa acccaaggag cgccgcatct cactcggcat
     6601 caagcaactc gaagcggacc cctggacgac ggtcgcggac cgctacagca ttggctcgct
     6661 tgtcgaagga cgcgtgcgca aactgacgga cttcggcgcc ttcatcgaaa tagaggaagg
     6721 cgtggacggt ctcgtccaca tctccgatct ttcctgggtg aagcgcgtca agcatccatc
     6781 ggagattctg aaaaaaggcc aacttgtgca ggctgtgatt ctcaacatcg acgcgtcgaa
     6841 tcgccggctc tccctggggg tgaagcaact gcaaccggac gcctgggaaa cattcttccg
     6901 ctcgcaccag gtgggcgata tagtacgcgg agtcgtctgc cgctccgcta atttcggagt
     6961 gttcgtcgaa ttgagccccg gcgtcgaagg cctctgccat aactcggaga ctccgggagc
     7021 ggccgaccgg cggaaggatg aaccaccggt ccccattggc caggagttcg atttcaagat
     7081 catcaaaatg aacgaggccg aaaagaagat cggccttagc atccgcgctg tggcggacga
     7141 cgaggagaga gttcgcctcg aggattacca gcgccaggcc gctgccgcaa ctatgaccat
     7201 cgaggaagtc atgaacctga aagaacaggg tgagggccgc tgaatgcggc tcgcggcgga
     7261 atccattccc ggcgaatcaa taatacacaa ggagacaacg gaatgacgaa agcggatctg
     7321 attgaggagg tctcgagagt tgtcgagatg actcgcaagg attcggaggt aattgtcgag
     7381 gcgatcttcg acagcatcgt gcggtcgctg cgcaccggag acaagatcga gatccgcggg
     7441 ttcggaagct tccggacccg tcagaggcag ccgaggatcg gacgcaatcc gaaaacaggc
     7501 gcccgggtgg aagttccatc caaacgtatc ccgtatttca aaccgagcaa agagcttaag
     7561 gacctggtga acgaatcggc agccgccgaa gcgggcaacg cggaagcgcc cccgccccct
     7621 gcgccggcgg aagaaacgcc gcatggaatg tagcgcgtca gggtatggac cggttatgga
     7681 gtccctggag gttcaactac gtcagcaagg ctgagatccc tcgcggctgc ctcttttgcg
     7741 acaaaccagc ggaacaggac gaccggaaga accttattgt ctatcgtggc gaccggactt
     7801 tcgtcatcct gaaccttttt ccttacactg ccggccacct gctgatcgcc ccgtacgcac
     7861 acatcgccac tcttcaggac gcatccgacg agatcgctac agagatgatc cgactggcga
     7921 gacttgcgga gaaacacctt cggactattt ataagcccga ggggctcaat atgggcatca
     7981 acctcggaga gtgcgccgga gcgggagtgg cggggcatct gcacatgcac gttcttccca
     8041 gatggtgcgg cgatgccaat ttcatgacca ccgtcgggga aacccgcgtg ctcccggaag
     8101 acataaacgt tactttcgag aagctcgccg gcgccttccg tggaagctga ccttattctc
     8161 cgaacctgaa cccatgaaac aggccaatct ctttgcggag ctggcggcgg agttcgcggg
     8221 tactaccgtc ttaatcctct ttggaaacgg tgtagtggcg atggttctgc tgttcggcac
     8281 cggtgtaccc ggcgaagtcg tcaaaggggg cttcacaaac atcactattg cctggggcct
     8341 cggcgtgacg atgggaatct acgtggcggg ccgcatcagc ggagcgcatc tgaatccggc
     8401 cgttaccctc gcgctcgcct tgttcagagg atttccatgg cgaaaagtag tgccctattc
     8461 gattgcccag accgccggcg cgttttctgc tgccgcccta gtgttctgga actaccgccc
     8521 ggccttcgaa cgcgtcgatc ctaacctgga acgaactgct gcgatcttca ccacgtttcc
     8581 ggcatttggg gattttccgg cggcgggctt tctcgaccag gtcatcggca ccgcattact
     8641 gctgttcctg atcttcgcgc tcacggacga aaacaaccag cagggcgccg cagacctgat
     8701 gcccttgctc atcggattga ttgtagttgt gatcggcatg tctttcggtg gcatgcacgg
     8761 gtatgccatt aatcccgcgc gtgatttcgg tccacgggtt ttcacggttc tcgccggctt
     8821 ccgcaacaat ggactgaccg acggcacctg ggcgttttgg atacccattg ccggaccgat
     8881 gctgggagga gcactcggag cggccgcctg ggaccttgga atcggccgct gtctgccaag
     8941 cgccgtccgg gatcgggtgg aacggtgatt tacggctata atgatagcta acgccccaag
     9001 catcttgcca agaggtgtgt gttgttccta agtgtcaagg aaatggaagc caggaaagtg
     9061 cgtttcgacg tcactttccc tcctggagag attgagtttc acgatagaaa actccgccag
     9121 attacgccgc tccaggtaga aggttcagca gagctgatgg gcgtcacgca ggagatacgc
     9181 gtgcggggcc ggctttcggt gggtatggag gccgattgcg accggtgcct cgaaccggcg
     9241 ccgtttcccc tggaaagcga gtttgacctc ttctaccggc cttccgagga gacggcggga
     9301 gatgagaagc tgttggacga agacgagtct gagatcggct tttacgaaga aggcggcctg
     9361 gacttgaagg acatcatgcg cgagcagatt ttgctgtcgc tgcccatgca gcggatctgt
     9421 gacgaagcgt gtaagggcat ctgtccggta tgcggcggaa atcgtaacat ctccgtatgc
     9481 ggatgccacg aaaagaaact ggacgaccga tgggcggcgc tgcgggaact gaaacagcag
     9541 aagccgtgaa tacttgagac tggaaggatt atagatgcca aaccccaaac gcagacattc
     9601 aaaacgccgc acctctaccc ggagggcaca cgactcgctg cgcgtttctt ctcacgccga
     9661 gtgcccgaac tgccatgaac cgaaactgcc gcatcgtgta tgcccccatt gcggcaagta
     9721 caagggtcga gagatcatcg aagtcgctga agaataatcc gcccgagagc cgctctcccc
     9781 catggtgacc atcgctgttg atgcaatggg cggcgaccat gccccaaaaa ccgaggttga
     9841 aggggccatt cgcgccgcca gaagtctggg aatccgcgtc atcctcgttg ggcagcagga
     9901 tctgctccaa cgggaactgg cccaacacga tggagtgaac catctgtcgg ttgaaattca
     9961 tcatgcgagc gaacgcgtga ccatggacga tagcgccgct cgtgccgttc gtacgaagcg
    10021 cgacagttcc atccgcatcg cctcgcggct ggttcgcgaa ggaatcgccg acggcgtcgt
    10081 ctcggccggc aacaccggtg ccattatggc cacggtcaaa atggtacagg ggatgatccc
    10141 gggagtggat cgccccgcgc ttgcctccgc atttcccact ttgaagggaa cgccggcggt
    10201 aatggtggac gtgggagcca atgtcgattg ctcgccgcaa atgctggccc agttcgcggt
    10261 catgggcgaa atctattccc gggtgatttt ccgtaccgag agcccgcgcg tcggccttct
    10321 ctcgatcggc gaggaggaac acaaagggaa cgacttgacc cgctcggcta cgcctcttct
    10381 gaagactctg cggctgaatt tcatcggtaa tgtggaaggg cgcgacgttt atacgggcac
    10441 ggcggacgta atcgtttgcg acggtttcat cggcaatgtc gccctgaaag tcagtgaagg
    10501 cctggtggat gtcatcaagc acatgcttca ggaatcgctg gaggctacca tcacccgaaa
    10561 aatcggctac gtgctctcga aagcgggctt cgcggatttc aagaagcgtg tggattattc
    10621 ggaatacggt ggagcgcctc tgttaggggt gaagggcgtt tgcattattt gtcacgggcg
    10681 ctcgaacgcg aacgcaatca aaaatgcgat tcgcgtggcg tccgaatttg cgtccggtaa
    10741 cgtcaaccgg cgcatcgagc aggaattgag ccgctggcaa gctgcggcga gagcggactg
    10801 aagtgaagtt gtcacgggcc attgcgacgc cgctggtcct ggcgttcctg gcggcgccag
    10861 ggttcgcgca aaagctgaat cctgtccagt ggagccttac gctggagccc tccagcgctc
    10921 ccccgggcgg gcaggttgcc gtacgcctga cagccgccat ggatgccgga tggcacctct
    10981 actctctcac cacaccccgg ccgcccatcg caaccaccat cagccttgca gagaatccgg
    11041 ccatcgccgg cttcaaggta tatcagcccg cgcccgtacg aaaactcgat cccaacttta
    11101 acaccgagac ggaaacattt gatggcaaag ctgtttttct gatcgtagcc accattgccg
    11161 gcgacgccgc cggcggccct gtggaactgg ccgccgggat tcgctaccag gcctgcgacg
    11221 acaaacagtg cctgcctccc aggcggaaca ccgttacagc aacgctcacc atagacccgg
    11281 ccgctcagcc cgcgcaaatc gcgattcccg ccggatactc ggagccgtcg tccggagcgc
    11341 catcttccgc tcagacccct gcgccgccac ccccggcagc cggcggcgaa ccacaaccgt
    11401 tcggaacgtt tctgctggtc gccttcggat tgggactcgc cgccattttc acgccgtgcg
    11461 tcttcccgat gatccccatc acaatgtctt tttttctgaa gaagcaggcg gcgacccgga
    11521 gggaaagcat cgttcaggcg ggcgtgttcg cgctgggtat catcgtgctc ttttcaggat
    11581 tgggtctgct cacgacggcg attctcggac ctttcggcgt cgtgcagttg ggatcgaatc
    11641 cctgggtgaa cacgtttatc gccgcggtgt tcctggtctt cggcctgagc ctgctgggtg
    11701 cgttcgagat tacgattccg tcgagcgtat tgacccgcct cgaccaggct tcgcaacgcg
    11761 gcggcatgct ggggacgcta ctcatggggc tgacgttctc gctgacttcg ttcgcctgtg
    11821 taggtccgtt cgttggtacg ctgctggccg cgtcggttca gagcggagga atacagccgc
    11881 tgctcggaat ggccgcgttt gccaccggcc tc
//
LOCUS       DASTMK010000191         9492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_105771, whole genome shotgun sequence.
ACCESSION   DASTMK010000191 DASTMK010000000
VERSION     DASTMK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105771"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="VFQ79_08310"
     CDS             <1..1750
                     /locus_tag="VFQ79_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139698.1"
                     /translation="ISLYDMDHATLFGGAGGPLFLKSTANLSGTASKSQAARVITVRL
                     REELGGITPDEWKKARQLAFKTTVLALSTEPGMVCSLTHADMPGGHGEFRVTSWRLNR
                     DYSIDIQGHTTTDSMYDLISGPKPADVMPDAPTGEILIDTGVPGILSGTPRLGDYGTF
                     AIDDMSVAPDASGNANIVGAHEVTLALYYVDELTTDLWASIDVNIDAATDPMTIACTV
                     NPDTMRVFRVGDFVVFNDESADPDNPGRRSYECVQITGPGAPGDVVPGGEFHLQRAYP
                     GVPEGQATFGTLRCAHLAGVRFCKLDQKTFTFSVRKGFFRTPDLPARIEATLPSACLI
                     AALADVANHFGYGPFTVFPLSRHNEPYMPGLRTCNGGAYTFQVPGPLTAQENVVIPLK
                     VQDAASIRCAYAYLQQGTTDGQSAFLVKISRDAGATWEPLEYMGIAQALPDAYKNTYD
                     FLVNNEGYGLPVTRRLPYADYGLVLISAVTAGPEPQTLQTASYGANRLGLEAGGFVFL
                     DPGGANEEYVHVIRVDPENQSFQAIVTKDHAAGERIRPTIWPTPALYEGDDLAFDLLA
                     VASPDPGSDLTVVIQT"
     gene            complement(1772..2152)
                     /locus_tag="VFQ79_08315"
     CDS             complement(1772..2152)
                     /locus_tag="VFQ79_08315"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEU0139699.1"
                     /translation="MRFLVDNALSPELARLLRAAGHDAVHVRDYELHAAEDPIILERA
                     GAEDRILISADSDFAMLLATSRRTKPSFVLFREPDIIRPADYANAILLNLPAIEHDLL
                     AGCVATFRHGRVRVRNLPIGGPQQ"
     gene            complement(2149..2388)
                     /locus_tag="VFQ79_08320"
     CDS             complement(2149..2388)
                     /locus_tag="VFQ79_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU0139700.1"
                     /translation="MQVTNFERITVNPNQMGGVPCIRGLRIPVATVLRMLAGGMTEQE
                     ILADYPDLRSEDIRECLRFAAVSAMERDLPLSRTA"
     gene            2439..6344
                     /locus_tag="VFQ79_08325"
     CDS             2439..6344
                     /locus_tag="VFQ79_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139701.1"
                     /translation="MSADPLHIFDPRRTVQLQGFSGRAATTTLHDATAIGFQLSGIFQ
                     AAEDFANIQLFSAYDYFNHLRVKPLPVTDLSGLTLQYDMEILPVNGEEGNVRPDCVRY
                     ASVGWDKLTITTGAGDIYEIPLMHHAQIVSGDYDPGGFGFSLHDRDADTLDELLVGKP
                     TPALTDKAYVYFMGTRWSCSSAEAIAFCNLETRLLNNISAPDVPSCEQAIWWQEDPNF
                     WHYLRVNNDAAGIQEADATDATDIASRLASMVGISSYLVDCIASGNVITVTLEPGVNG
                     PVQVSTNSRSAPATLSRSGPGIYTAQVASSSEIRVGDYVGIDIGSANDEVVKVLAVGP
                     GTFTAYFTKPHYGRISNIQCRVLPRARHFGRVLKECIADGPLLDYGEQPNNLTVEQFT
                     MTNTSCELRLRLVGQLGEYGRDANGMPVRASVDSENKIVRIEKNDDEFGATSIATAVE
                     GAGNSRAYRFTLPFPLLSGYLNGNRNSLVPVPAGNIVKVHLTFAPRFEDVEAGLAVGG
                     LLKEGVSTSPAGTEEEWRVADAEAMLAGLKYYVGTANAEERITCLANFGLVQPDLDDT
                     ATWYYRLLVRRGEDSSMPQAWPPGTRIQRISTITGTRSDIEWQVRISNIAVTGDRSLK
                     VGGDAPRIEESDGRCRYEGFWENYAYGASWPTQWWSRGHARRCAPNDAQDQRAVTIRY
                     SYPSQHDLYLGTWLGRDAGRIQVVIDGGAPLVHDLYLNDYNGLAAMKKLAAALPGGTH
                     TVEIRALFDKHPASNGYYFYFDYLWPLEPQDPPDPPKVYNDVSLAIDFDTDHGYKKPP
                     AWHVWHLKKLGFLGHADVYLGVFWNNKRRRIEATYPNCTASIGGWRPDEPLWINLSGT
                     TLYFAPGTGLETKDIAAHLRAMINVTFPGVWCTSDGGSIHIRSRAPSYTFTISASPQL
                     SVSQGTGTYPKVTVDGKGRVTAGTNLAADDLPTHAHSAGDIVSGEFPHKIQKDGDDVG
                     TRGALNLVQGNRISLATADDPANDRVSMTISASPPEAGEITNALGYVPANRTGEHFTG
                     PIDCGPHQTIGGPLENMAKHSEDFAAAVWDKNGGSCSVTSNAIIAPDGNQTADVVTVV
                     TTTPVIQQQIAGLADSGTYTFYIRARVASGTRKVSIAIVDNPYAAYLAGPTQIMLTTA
                     WQRFKITGTLASGQTGLWIVVRQFAANGDDWTTGAIHLWGTCLQHGDDPQKAYARAWA
                     SQTLHSASGIAAGPTVIAASDTTTSPLKIHGPGSNLADSTLLELTANGELILAGGSGN
                     GYRFAELAAANNPSGWAGVLKVKTPAGTTLGYILLHTNP"
     gene            complement(6329..6490)
                     /locus_tag="VFQ79_08330"
     CDS             complement(6329..6490)
                     /locus_tag="VFQ79_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139702.1"
                     /translation="MVDGLLLLSRECKLVLKGPEGADGGDIAALGVKGGGSNVQTLNV
                     IKLDLHGFV"
     gene            complement(6696..7802)
                     /locus_tag="VFQ79_08335"
     CDS             complement(6696..7802)
                     /locus_tag="VFQ79_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0139703.1"
                     /translation="MPVSYTEVTAKSLINSIPHSKFSVLKGLSCRSSMNVAFGCETFC
                     SFCYIRFMTRWKGMEPNDIFQRIQIRTNAPELLEKELRRRPKEWIWVGSTSDPYQDYE
                     EQYGLMRRCLEVFREYHYPFEIITKSPLVARDADLLGELQDVGLVSVSFTSINENKRK
                     GVELRARSTLERLDALRKLNAAGVRTCALLLPIIPGHTDAEPELEDLIRKIVAAGTTN
                     LYAGVMRLYPITWAGMRKAMAPRLAALRNDIEQRYYETTKELSAGARVPDRQYRRALM
                     HRVGEIGKAAGIVSYSCEDNFFDLWYGLQDDHDIYRYAVHYDFWRERRELRVPALTLE
                     QAMAVARRFRHTPAYLRSIEQNLNLLNRLISVDV"
     gene            complement(7812..8102)
                     /locus_tag="VFQ79_08340"
     CDS             complement(7812..8102)
                     /locus_tag="VFQ79_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139704.1"
                     /translation="MLDWEMAAVADPVIDIAWLFARQGWSRKKREAFFETYDMPVAPP
                     HLVDFLGQLCLLDQAVWAHSCLDDIEVGRHAEALGAAQIPFLRASAEFLETI"
     gene            8135..8434
                     /locus_tag="VFQ79_08345"
     CDS             8135..8434
                     /locus_tag="VFQ79_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139705.1"
                     /translation="MVADIPMDKHWRCYGGHPESQKTLSSIPDEHHGASELARRKVRG
                     KKSSVHVVQQVEYRRRQVFGGTRPPKMRWKGEPMKLCDDSAEARDGVTIKLNRIT"
     gene            8531..8680
                     /locus_tag="VFQ79_08350"
     CDS             8531..8680
                     /locus_tag="VFQ79_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139706.1"
                     /translation="MPKNAILPLNPLVDPRNVQQQSAQHGRAERQSFDEVMGVVEPPP
                     QKTDG"
     gene            complement(8801..>9492)
                     /locus_tag="VFQ79_08355"
     CDS             complement(8801..>9492)
                     /locus_tag="VFQ79_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0139707.1"
                     /translation="NAAVIGAVNSGLFAAGQACMSPERFLVPAASVQDFREALVSAFA
                     EWPQGDPFSREIRLGLIADGRIVEEVQRQVREAVSAGAKVVHGGQIRELSVAGITMWE
                     LQPTILVGVTSDMDMMQRETMAPIFPVYGYRTVEEAMTVAEGTRYGLTATVFGRGQVY
                     RDAAQRLARSHGLVFQNVDMVAGFEPQHWGSGGFKRSGWIWEWRDGAFVRREGRRDLS
                     AEWHRIATGAK"
BASE COUNT         1864 a         3133 c         2760 g         1735 t
ORIGIN      
        1 catctcgctt tatgacatgg accacgcaac gttgttcgga ggcgcaggtg ggccgctgtt
       61 cctaaagtcc acagcgaatc tctccggcac ggcgtccaaa tcgcaagccg cacgggtcat
      121 cacggtccgc ctgcgtgaag agttgggcgg catcacaccg gacgagtgga agaaggcacg
      181 gcaactcgcg ttcaagacga cggtactcgc cctgagtacc gagccaggca tggtctgctc
      241 gctgacgcat gctgatatgc ccggcgggca cggcgagttt cgcgtcacct cgtggcggct
      301 gaaccgcgac tactcgatcg atatccaggg ccacaccacc acggactcga tgtacgatct
      361 gatctccgga cctaaacccg ccgacgtcat gccggatgcg cccactggcg agattctcat
      421 cgacaccggc gttccaggca ttttgagcgg tacgccacgc cttggtgatt acgggacctt
      481 cgccatcgac gacatgtccg ttgcgcccga tgcctccggc aacgcgaaca tcgtcggggc
      541 ccacgaggtt acccttgcgc tttactatgt cgacgaactg actacagatc tctgggcgtc
      601 gatcgatgtg aacattgacg ccgcgaccga tccgatgaca attgcctgca cggtgaaccc
      661 cgacacgatg agagtcttcc gtgtcggcga cttcgtcgtc ttcaacgacg agtccgccga
      721 tccggacaac cccggcagga gatcctatga gtgcgtccag atcaccggtc cgggcgcgcc
      781 cggcgatgtg gtgcccggag gcgagttcca cctgcaacgc gcctaccccg gagtcccgga
      841 gggtcaagcc acattcggca ctctccgttg cgctcacttg gccggcgtcc gtttctgcaa
      901 actggatcag aagacgttca ccttcagtgt ccgcaagggt ttcttccgca cgccggacct
      961 gcccgcgcgc atcgaagcta ctcttccgag tgcctgtctt atcgccgccc tcgccgatgt
     1021 ggcgaatcat ttcggctacg gtcctttcac cgtgtttccg ctctcgcgcc acaacgaacc
     1081 ttatatgccc ggccttcgga cctgcaacgg cggcgcctac accttccagg taccgggtcc
     1141 gctgacggct caggagaatg tcgtcatccc gttgaaagtg caggacgccg cttcaatccg
     1201 ctgcgcctac gcttacctcc agcagggcac aacggacggc cagtcggcgt ttctcgtgaa
     1261 gatcagccgg gacgcaggcg caacgtggga gccgcttgaa tatatgggaa tcgcgcaggc
     1321 gttgccggat gcctacaaga atacctatga cttcctggtg aacaacgaag gctacggcct
     1381 gcccgtcacg cgccgcctac catatgccga ttacggcctg gtgctgatct ctgccgtgac
     1441 cgccggccca gagccgcaaa ccctccagac ggcctcctac ggcgcgaacc ggctgggcct
     1501 tgaagctggc ggcttcgtct tcctcgatcc cggcggcgcg aatgaggagt acgttcacgt
     1561 gatcagagtc gatcccgaga atcaatcttt ccaggcgatc gtgaccaagg atcacgccgc
     1621 cggcgagcgc atccggccaa ccatctggcc aacaccggcg ctctacgagg gtgatgactt
     1681 ggcgttcgat cttctggcgg tcgcctcgcc ggaccccggt tcggacctga ccgtggtgat
     1741 ccagacgtga cgatgaactg tatgaagtcg actattgctg cgggccgccg atcggcaaat
     1801 tgcggacgcg gactcggccg tggcgaaacg tcgcgacgca cccagccagg agatcatgct
     1861 cgattgctgg cagattcagg aggatcgcgt tcgcgtagtc cgctgggcgg ataatatccg
     1921 gctcgcggaa cagcacgaaa gagggcttcg tacgtcgcga ggtcgccagc agcatggcaa
     1981 aatccgagtc tgccgagatc agtatccgat cttccgcccc tgcccgttct aggatgatcg
     2041 gatcttcggc cgcgtgaagc tcataatcgc ggacgtgaac ggcgtcgtgg ccagcggccc
     2101 tcaagagccg cgccaactcc ggcgacagcg cgttatcgac aaggaacctc acgccgtacg
     2161 cgagagaggc agatcacgtt ccatcgcgga gaccgcggcg aaccgaagac actcgcgaat
     2221 gtcctcactc cggaggtcgg gataatcggc gaggatttcc tgctccgtca tgccgcccgc
     2281 aagcatccga agcaccgtag caacagggat gcgcagaccc cggatgcagg ggacgccacc
     2341 catctgattg gggttgaccg taatgcgctc gaaattggtc acctgcatgt gtagcctcgc
     2401 cctattatcg caggtcacag gccaagttag ataccggcat gtccgctgac ccgctccaca
     2461 tcttcgatcc tcgccgtact gtccagctcc agggcttctc cggccgcgcc gcgaccacca
     2521 cgctccatga cgccaccgcg atcggtttcc aactctccgg cattttccag gccgccgagg
     2581 acttcgccaa catccagctc ttctcggcct acgactactt caaccatctg cgtgtgaagc
     2641 cgctcccggt gacggatctt tccggactga ccctccagta cgacatggag atcctgccgg
     2701 tcaacggcga ggagggcaac gtccggcccg actgcgtgcg gtacgcctcg gtcgggtggg
     2761 acaagctcac catcaccacc ggcgcgggcg atatctacga aattcctttg atgcaccacg
     2821 cccagatcgt ctcaggagat tacgatcctg gcggctttgg cttctcgctt cacgatcgcg
     2881 acgcggacac gctcgacgag ttgctcgtcg gcaagcccac gccggccctg accgacaagg
     2941 cttacgtcta cttcatgggt acgcgctggt cctgctcctc ggctgaggcg atcgccttct
     3001 gcaatctcga gacgcggctg ctcaacaaca tcagcgcacc cgacgttcct tcctgcgagc
     3061 aagccatctg gtggcaggag gacccgaact tctggcacta cctgcgggta aacaacgacg
     3121 ccgctggcat ccaggaggcc gacgcgacgg atgctaccga cattgcctcg cgcctggcct
     3181 cgatggttgg tatttccagc tacctggtcg actgcatcgc ttcgggcaac gtcatcacgg
     3241 tcaccctgga gccgggcgtg aacggcccgg tgcaggtttc gaccaatagc cgctcggcac
     3301 ccgccacgct gagccggtcg gggcctggca tctacaccgc acaagtggca tcctcgtccg
     3361 aaatccgtgt gggcgactat gtcggcatcg acatcggcag cgccaacgac gaggtcgtca
     3421 aagtcctggc cgtgggtccg ggcaccttca ctgcatattt cacgaagcca cattacggaa
     3481 ggatcagcaa catccagtgc cgcgtcctgc cgcgagcgcg gcacttcgga cgggttctga
     3541 aggagtgcat tgcggatggc ccgctgctcg attacggcga gcaacccaac aacctgacag
     3601 tcgagcagtt cacgatgacg aatacatcgt gcgaactcag attgcggctc gttgggcaac
     3661 tgggcgagta cggccgtgac gcgaacggca tgcccgtacg tgcatcggtc gatagcgaga
     3721 acaagatcgt ccggatcgag aagaacgacg atgagtttgg cgcgacttcg attgccaccg
     3781 ccgtcgaagg cgccggcaac agtcgcgcct accgctttac gttgcccttc cccttgcttt
     3841 ccggctactt gaacggaaac cgcaattcgc tcgtacccgt gcccgctggc aacatcgtga
     3901 aggtccacct taccttcgcg ccgcgcttcg aagatgttga agcaggtctg gcggtcggtg
     3961 gtctgctcaa agagggagtc tcgacgtctc cggcaggcac ggaggaagaa tggcgcgtcg
     4021 cggatgccga agccatgctc gccggcctca agtattacgt aggcaccgcg aacgccgagg
     4081 agcgaatcac ctgcctcgcg aatttcggcc tggtgcagcc tgatctggat gataccgcca
     4141 cgtggtacta ccgcctgctg gtacgccgcg gcgaggattc ctcgatgccg caggcctggc
     4201 cgccaggcac ccgcatccag cggatctcca ccatcacagg cacacgctcg gacatcgagt
     4261 ggcaggtgag gatctcgaat atcgctgtca ccggcgaccg gagtctgaaa gtcggcggcg
     4321 acgcgccccg gatcgaagaa tccgatggcc gctgccggta cgagggcttt tgggaaaact
     4381 acgcctatgg cgcaagctgg cccacgcagt ggtggtcgcg tggccacgcc agacgctgtg
     4441 cgcccaacga cgcgcaggac cagcgggcgg tcaccatccg ctactcctac ccgagccagc
     4501 acgatctcta cctcggcacc tggctcggcc gcgacgcagg ccggatccag gtcgtgatcg
     4561 atggcggcgc gcccctggtt cacgatctct atttgaacga ctacaacggc ctcgcggcga
     4621 tgaagaagct tgccgcagca ctgcctggcg ggacgcatac ggtcgaaatc cgggcgctgt
     4681 tcgacaagca tccggcgagc aatggttatt acttctactt cgactacctc tggccgctcg
     4741 agccgcagga cccgccagat ccgccaaagg tctacaatga cgtctcgctc gccatcgact
     4801 tcgacaccga ccacggctac aagaagccgc ccgcgtggca cgtctggcac ttgaagaaac
     4861 tgggtttcct gggccacgcc gatgtctacc tgggcgtgtt ctggaataac aagcgccggc
     4921 gtatcgaggc gacctaccca aactgcaccg cgagcatcgg cgggtggcgg ccggacgagc
     4981 cgctatggat caacctttcc ggcaccacgc tctacttcgc gcctgggaca ggtcttgaaa
     5041 cgaaggacat cgccgcgcac ctacgcgcga tgatcaacgt cacctttccc ggcgtctggt
     5101 gcacgagcga cggtggctca atccatatcc gctcccgcgc accgagctac accttcacca
     5161 tctcggccag tccgcagttg agcgtttctc agggtacagg cacctacccg aaggtcacag
     5221 tggatggcaa gggccgcgtg accgccggta caaatcttgc ggcagacgac cttcccaccc
     5281 acgcccacag cgccggcgac attgtgagcg gcgagttccc gcacaagatc cagaaagacg
     5341 gggatgacgt cggcacccgt ggtgcgctca acctcgtcca gggcaaccgc atctcgcttg
     5401 cgaccgctga cgacccggcg aacgaccgag tcagcatgac catcagtgcg agcccgccag
     5461 aggccggcga aatcactaac gccctcggtt acgtgccagc gaaccgcaca ggcgagcact
     5521 tcaccgggcc catcgactgc ggtccacatc agaccatcgg cggcccgctg gagaacatgg
     5581 cgaagcactc cgaggacttc gccgccgctg tgtgggataa gaatggcggc tcgtgctcgg
     5641 tgacgtcaaa cgccatcatc gctccggacg gcaaccagac cgccgacgtg gtcaccgtcg
     5701 tgaccaccac gcccgtcatc cagcagcaga tcgcgggtct cgctgacagc ggcacgtaca
     5761 ccttctacat ccgggcccgc gtcgcatccg gtacgcgcaa ggtgtcgatc gccatcgtgg
     5821 acaaccccta cgccgcgtat cttgctggcc caactcagat catgctcacg accgcgtggc
     5881 agcggttcaa gatcacgggc accctcgcat cagggcagac tgggctgtgg atcgtcgtgc
     5941 gccagtttgc cgccaacggc gacgactgga ccaccggcgc catccacctc tggggcacct
     6001 gtctccaaca cggcgacgac ccgcagaaag cctacgcccg cgcctgggcc tcgcaaacgc
     6061 tgcacagcgc ctctggcatc gcggccggcc ccaccgtaat cgccgcgtcg gacaccacca
     6121 cctcacccct caagattcac ggccccggct ccaacctggc cgacagcacc ctcctcgagc
     6181 ttaccgccaa cggcgaactc atcctcgccg gcggctccgg caacggctac cgcttcgccg
     6241 aactcgcagc cgcaaacaat ccctccggct gggcgggcgt tctcaaggtg aaaaccccag
     6301 caggcacaac gctcggctat attcttcttc acacaaaccc atgaagatcc agcttgatca
     6361 cgttcaacgt ttgaacgttt gaacctccac ccttgacgcc cagcgcggcg atgtcgccgc
     6421 catccgcgcc ctctgggccc ttcaggacaa gcttgcactc acggctgagg aggagtaggc
     6481 catcgaccac aagagggaat tcgtggcagg ccacgaacgc gttgcctgga attgctaggt
     6541 ggacggcagg ccgactatct gcgtcgtcct gcggacctca ggcgtcacct ccggcgaccc
     6601 atccgccaaa gccgatttcg ctcacggtcc tgccgaatgt ccgatattcc atttggcacc
     6661 ctattgaatc cggagattag cacgaaagag ccaggctaaa cgtccacgct gatcaaccga
     6721 ttgagcagat taaggttctg ttctatcgac cgaaggtagg ctggcgtgtg gcggaatcgc
     6781 cgagcgaccg ccatggcctg ttccagcgtc agtgccggaa cgcggagttc cctccgctcc
     6841 cgccagaaat cataatggac cgcgtatcga tatatatcgt gatcgtcctg cagaccgtac
     6901 cagagatcga agaaattgtc ttcgcacgag tagctcacta tgcccgcggc cttgcctatt
     6961 tccccgacac gatgcatgag cgcccggcga tactggcggt ccggcacccg ggctcctgcg
     7021 ctaagttcct tcgttgtctc gtagtaccgc tgctcaatgt cgtttcggag cgcggccagg
     7081 cgcggggcca tggccttgcg catgccggcc catgtgatcg ggtaaagtct catgacgccc
     7141 gcataaaggt tcgtcgtccc ggcagcgacg atcttcctga tgaggtcctc caattccggc
     7201 tcggcatcag tgtgcccggg gataatcggc aacaagagcg cacaggtccg aacgcccgcg
     7261 gcgttcagtt tccgcaatgc atccaggcgt tccagagttg aacgcgcccg gagctcaaca
     7321 cccttgcgtt tattctcgtt tatgctcgtg aagctgacgc tcaccaaccc gacgtcttga
     7381 agctcgccga gcaggtccgc atcgcgggcg accaggggac tcttggtgat gatctcgaac
     7441 ggatagtgat attcccggaa gacttccagg cagcggcgca ttaggccata ttgctcttcg
     7501 taatcctggt aggggtcgct tgtgctgccc acccagatcc actccttcgg gcgccttcgc
     7561 agctcttttt ccaggagttc cggggcgttt gtgcggatct gaattcgctg gaaaatgtcg
     7621 tttggctcca tacccttcca ccgggtcatg aaacggatat agcagaacga gcagaacgtt
     7681 tcgcacccga aggccacgtt catgctggaa cggcagctca gacccttcaa gacgctgaac
     7741 ttcgagtggg gaattgagtt gatcagcgac ttggctgtta cttcggtgta gcttactggc
     7801 acattgcctc cttatattgt ttccaagaat tctgcggatg cccgaaggaa ggggatctgc
     7861 gcggccccga gggcctccgc gtgccgcccc acctcgatgt cgtccaaaca ggagtgggcc
     7921 cagaccgcct gatcaagcag gcagagctgt cccaggaaat cgaccaggtg cggcggtgcc
     7981 acgggcatgt cgtaggtttc gaaaaacgcc tcgcgtttct tgcggctcca cccctgcctg
     8041 gcgaagagcc aagcgatgtc gataaccggg tcggcgactg ccgccatttc ccagtccagc
     8101 atcactaggg tccggccgac cgggagagga ggttgtggtc gccgatatcc ccatggacaa
     8161 gcactggagg tgctacgggg gccacccaga gtctcagaaa acgctttcga gcatccccga
     8221 tgagcaccac ggcgcctcgg aattggcgcg gcgaaaggtg agaggcaaga agtcttctgt
     8281 ccatgttgtc cagcaggtcg agtaccgccg ccgtcaggtc ttcgggggca ccaggccgcc
     8341 gaagatgcga tggaaggggg agccgatgaa gttgtgcgac gattctgccg aggcgcgcga
     8401 tggtgtcacc atcaaactga accgcatcac ctgactcgta cgtcattaag agccacggct
     8461 ggccaaacgc ccttccttcc gcatcacagc cgagcggcct cggcgcgtgg gcatatccgt
     8521 actctgccaa atgccgaaga acgcgatact cccgctcaat ccgctggtgg atcccaggaa
     8581 cgtccaacag caatcggcgc agcacggccg ggccgaacgg cagtccttcg atgaggtgat
     8641 gggagttgtt gaacccccgc cccagaaaac ggacggatga agccgagaag tgccgctgta
     8701 tcgcgttccg caagggaacg ctatctgcct catggaagtc gggaacctcc agcggcaaat
     8761 cccgccagtg ttcaacccaa ctgggccaga gatgcacgga ctacttcgct cctgtcgcta
     8821 tccggtgcca ttccgcgcta aggtcccgtc tgccttcccg gcgcacaaac gcaccgtcac
     8881 gccattccca gatccacccg gaccgtttga agcctcctga accccagtgc tgtggctcga
     8941 aacccgcgac catgtcaacg ttctggaaca caagaccgtg ggagcgggcg agccgctggg
     9001 ctgcatcgcg gtaaacttgg ccgcggccga acacggttgc ggtaagtccg taccgtgttc
     9061 cttcggcgac agtcatcgcc tcttccacgg tgcgatagcc gtacacggga aagatcggag
     9121 ccatggtttc ccgttgcatc atgtccatgt cggaggtgac gccgaccagg atggtcggtt
     9181 ggagctccca catggtgatt cccgccacgg acagctctcg gatttggccg ccatgcacga
     9241 cttttgcgcc ggccgatacc gcctcccgaa cctggcgttg cacttcttcg acgatacgac
     9301 cgtccgcgat cagaccgagg cggatttcgc gggagaaagg gtcgccttga ggccattctg
     9361 cgaacgcgga gaccagggct tcccggaagt cttggacgct ggcggctgga accagaaagc
     9421 gttccgggct catgcacgcc tgccccgctg caaacaaccc ggagttgacc gcgccgatca
     9481 cggcggcgtt ga
//
LOCUS       DASTMK010000192         6815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_457151, whole genome shotgun sequence.
ACCESSION   DASTMK010000192 DASTMK010000000
VERSION     DASTMK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457151"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFQ79_08360"
     CDS             <1..747
                     /locus_tag="VFQ79_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139708.1"
                     /translation="KNRVLRLLPSTKVPFETWKAPVKSFVDRYCDASDVEFTTEKDIR
                     VQGQIFIPREEKYRQSALIYVKRSDDIVHPIDWDPLLPLFGRRVVLVLHPRGVDYPVT
                     NLQLSTIKRTAALLGGTLETMQLWDILRSVDFLTAEQGLNLKSISIYGRGQMGPLGFY
                     AAALDNRITRVILDDPPGSHWQGSPLLNILRITDLPETAALLAPRELVSLTPLPHAYD
                     YTASIFALYGKRDAIRQAGGLGEAVRVWEP"
     gene            752..3433
                     /locus_tag="VFQ79_08365"
     CDS             752..3433
                     /locus_tag="VFQ79_08365"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0139709.1"
                     /translation="MKDKGITKRDFLQTMGAGVPTVLALNPGLAAAQTVTGHPPAVTR
                     KAEPIDCSNYFTASAADLGPREQAARLTRESQQDRLIRMPGGERGLRGLPFRLGPEDV
                     NRKSWIVLRQNGEKWAAAQVEIPVDQAASFVCMAAFADWTTTASTAADPAWARGHHLA
                     DLTLAYEDGSEQRYPVRWGFEVNPPLVGFGEECYKAVPHRELVSSSLGDPLSNASLWG
                     LLQTGVYPLDRSDPYHGSLWIAAFPSPHPNRKIRKIRFDARGEDLLFVCGLTVYKGAE
                     HPLRLDRSRTYRFTLPEGQQGRRIDWDLDVDLGVVVKVYRLPTFDGESWLASPLAGLG
                     ESSEYLAGDRYLYAEVAAAPDAVVTLRNKQGGPAFLFDLSDVASGRETAPRPSQPRIE
                     WIEPHNAWIRGRVLDASTERPTPVCLAFRASNGRYIPPYGHRAEINDGWFQDYGADVK
                     RDGTSYAYVDGTFQVELPVGEVFVEVLKGFEYQPIRRKLRIEPNQRELSLEISRFSNL
                     RAEKWASADTHVHFLSPSTAVLEGQAEDLNLIHLLAAQWGDLFTNVGDLTHRSLRSPD
                     GEMIVHVGTENRQHLLGHLSLLGAQGDPVFPMSADGPGEGQIGMPLWSSLAEWAERSR
                     RNDGLAVAVHFPLPIGELAADIALGKIDAVELMPRLVTEEFETLPFRDWYRYLNCGYR
                     LPAVGGTDKMRASNAVGFNRTYTYLGDDEFSVQNWSKAVRRGNTFMSSGPLLLFKADG
                     RAPGEEISFRAGGGRVAVEAHARCMAPIHRLEIVRNGEVVASQVEERGARELRLSETI
                     EVSGPGWLAARCFSRFQSSGSRIAAHTSPVYITVPGRELFSAPVASYMLRLIDGADSW
                     VRELATRPDPETLERMFRVLRDAKAAVEERLRRLATP"
     gene            3769..4869
                     /locus_tag="VFQ79_08370"
     CDS             3769..4869
                     /locus_tag="VFQ79_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0139710.1"
                     /translation="MATQRTIPWFSAILIVAVGSTASSSTQTGGVSPDLVDRVSKAVV
                     LIKGTGENGTITGSGFLVSSDGKLVTNLHVIREMKRGGVQLHSGETFDSFSVLAFDER
                     RDLTILQIAGFDLPTIELGNSNDAKTGEAVIAIGSPRGLNGTVTAGVISAIRDDASTG
                     FKIIQTDAAANPGNSGGPLINVRGQVIAVVTSKLRASEGLNFAVPVNYVRGLLNIANT
                     PLTLDALRAALAGGGTDIFRQETAFPTSWKSLISGNVYNIRRQSDLIYVERLFPPEAV
                     KVGFFSAWELRKKEQQYVGSIRSVVVCEYQPWIETIVKRCPFEFRAEFSLFTDSRIEG
                     RTLAPRNGAKLNCRKCEYNKPPVWQSFTWIPQ"
     gene            complement(5039..5311)
                     /locus_tag="VFQ79_08375"
     CDS             complement(5039..5311)
                     /locus_tag="VFQ79_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139711.1"
                     /translation="MGKAIPIPPFELLDLLDREVCQAEAGMAKNKERLVLRNLIRLVI
                     TEITAEGVNDAEKKQSRRIAQASAIRFPVLQAHFRRKQVFLYRFHG"
     gene            complement(6123..>6815)
                     /locus_tag="VFQ79_08380"
     CDS             complement(6123..>6815)
                     /locus_tag="VFQ79_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139712.1"
                     /translation="WSWAKSLTDCQSDSEQGCGVENSYARHSEYGNVSYQPRHRLVGS
                     LLWDLPFGRGRRFANSLPGVAQWVLGGWTLSSTLAAQTGSFFNPTFNGFDVSNTNVTS
                     GRPDRLADGNLPSSERALQRWFDSTAFVVPGDMDGDGRPDVNVGRFGNSAPNILVGPG
                     TFDVSGGLRKEFQLTERVRLMVEGTFRNVFNHPNYSTPATNIRAANVGTITSLNGLAG
                     PRVGQLGARLEF"
BASE COUNT         1413 a         1918 c         2030 g         1454 t
ORIGIN      
        1 aagaaccggg tgttgcgact gctgccgtcc acaaaggtcc cctttgaaac ctggaaagct
       61 cccgtgaaga gttttgtgga ccgctattgc gatgcatccg atgtcgagtt cacgacggaa
      121 aaggacatcc gggtgcaagg ccagatcttc attcctcgtg aggaaaagta tcgccagtcg
      181 gcactcatct acgtcaaacg atcggatgac attgtccacc cgatcgattg ggatcccctg
      241 ctgccactgt ttggccggag ggtggtgctg gtgttgcacc cgcggggcgt cgactacccg
      301 gtcactaatt tgcagctctc gacgatcaaa cgaacggcgg ccctgctagg gggcacgctc
      361 gagaccatgc agttgtggga cattcttcgc tcggtggatt ttcttacggc cgaacagggc
      421 cttaacctga agtcaatttc catctacggg cgcggtcaga tgggaccgct cgggttttac
      481 gcggccgcgc tcgataaccg catcacccgc gtgattctgg acgatccacc cggctcgcac
      541 tggcaaggtt cgccgctgct gaacattttg cggataaccg atctgcccga gaccgcagca
      601 ttgctcgccc cccgggaact cgtcagcctg acgccgctgc cgcacgctta tgattacacc
      661 gcttcgatct ttgctcttta tggcaaacgc gatgccatac ggcaggcggg cggtctgggc
      721 gaagctgtgc gggtatggga gccgtagcgg aatgaaagac aaaggaatca cgaagcggga
      781 ttttcttcag actatgggcg ccggcgtgcc gaccgtactg gctctgaatc cgggtctcgc
      841 ggcggcccaa accgttaccg gccaccctcc cgctgtgacg cggaaagcgg aacccatcga
      901 ctgctcgaac tacttcacgg cctcggcagc cgacctcggg ccgcgagagc aggccgcacg
      961 cttaacgaga gaatcgcaac aggaccgttt gatccgcatg cctggagggg aaagggggtt
     1021 gcgaggtctc cccttccgct tggggcccga ggacgtcaac cggaagtcct ggatcgtcct
     1081 ccggcagaac ggcgaaaaat gggccgccgc ccaggtggaa attcctgtgg atcaagcggc
     1141 gtcgttcgtg tgcatggcgg ctttcgccga ttggacaaca acggcgagca ctgccgccga
     1201 tccggcatgg gcgcgcggcc accacctggc tgatctcacc ctggcctatg aagacggctc
     1261 cgagcagcga tatcccgtac gctggggctt cgaagtaaat cctccgctgg tgggattcgg
     1321 cgaggagtgt tataaagccg tgccccatcg cgaactggtg tcgagcagtc tcggcgatcc
     1381 tttgtccaac gcatccttgt ggggtcttct gcaaactggc gtttatccgt tggacagaag
     1441 cgatccgtat cacggctcgt tgtggatcgc ggctttcccg tctccgcatc cgaaccggaa
     1501 gatccggaag atccggttcg atgcccgtgg cgaggatctg ctgtttgtct gcgggctcac
     1561 cgtttacaaa ggagcggaac atccgctgcg cctggatcgc tcgcggacct atcgttttac
     1621 tcttccggag ggacaacagg gacggcgtat tgattgggat ctcgatgtcg accttggcgt
     1681 tgtcgtaaag gtctaccgtc tgcccacctt tgacggcgaa tcgtggcttg cgtcgcccct
     1741 tgccggtctc ggtgagtcct ctgaatatct cgcgggcgac cgctatctct atgccgaggt
     1801 tgcggccgca ccggatgcgg tagtgacgct gcgaaacaag caaggtggcc cggcgttcct
     1861 cttcgacctc tcggacgtcg catccggacg tgagaccgcg ccccggccgt cgcagccgcg
     1921 aatcgagtgg attgagcccc acaatgcctg gatccggggc cgtgttctcg acgcgtcgac
     1981 cgaacggcca acgccggtct gtcttgcgtt tcgtgcgagc aacggccgct acatcccgcc
     2041 ttacggccac cgggccgaga tcaacgacgg ctggtttcag gattacggcg ccgatgtcaa
     2101 gcgggatgga acgtcgtatg cctatgtcga cggcacgttc caggtcgaac tgccggtagg
     2161 agaagtcttc gtcgaggttc tgaaaggttt tgagtaccaa cccatccggc gcaagctgcg
     2221 catcgagccg aaccagcggg aactatcgct cgagatctca cgattctcca atttgcgcgc
     2281 ggagaaatgg gccagcgccg acacccatgt tcactttctc tccccatcga cggctgtact
     2341 cgaagggcag gccgaggatc tgaatctgat tcacctgctg gcggcgcaat ggggcgatct
     2401 tttcaccaat gtcggcgatc ttactcacag atcgctgcgc tcgccggatg gcgaaatgat
     2461 tgtccatgtc ggcacggaaa atcgccaaca tctgttggga cacttaagtt tgctcggcgc
     2521 tcagggagat cccgttttcc cgatgtcggc ggatggcccc ggagagggac aaatcggcat
     2581 gcccttgtgg agttcgctcg ccgaatgggc agagcgcagc cgccggaacg atggtctggc
     2641 cgtcgcggtt cacttcccgc ttcccattgg cgaactggcg gcggatattg cgttggggaa
     2701 gatcgacgcc gtcgaactga tgccaagact tgtgacggag gagtttgaga ccctgccttt
     2761 ccgcgactgg taccgctatc tcaactgcgg ttaccgcctc ccggccgtgg gaggaaccga
     2821 caagatgagg gcatcgaatg cggtgggatt caatcgcacc tatacgtatc tcggcgacga
     2881 tgagttcagc gtgcagaact ggtcgaaagc ggtacggcgc ggaaacactt tcatgtcctc
     2941 cgggccgctg ttgctcttca aggccgacgg ccgggcgccc ggcgaagaga tttcattccg
     3001 tgcgggcggc ggccgggttg cggtagaagc tcacgcccgt tgcatggccc cgatccaccg
     3061 gctcgagatc gtgcgcaatg gagaggttgt ggcatcccag gtggaggagc ggggagctcg
     3121 cgagctccgc ctcagcgaga ccatcgaagt gtcgggccca ggctggctgg ctgcgcgttg
     3181 tttctctcga tttcagtcgt ccggatcgcg gatcgcggcg cacacgtcgc ccgtctatat
     3241 cacggtcccg ggccgggaac tattttcggc tccggtagcg tcgtatatgc tcaggctgat
     3301 cgacggcgcg gatagctggg tgcgcgagtt ggcaacccgt cccgatccgg aaacgctgga
     3361 acgcatgttt cgcgttctcc gggatgcaaa ggcggcagtg gaagaaagac tccgccgtct
     3421 ggccactccc tgacgcgcgc ggctcggtca caactgctac gctagcaact tcccggccaa
     3481 aaatgagccg ttaaaccctt ccacgccaat gcctgctcca aggagttggt gcaagatcga
     3541 gcggatctcc gactgaaaca tttttgacta tccatgtcgt ttttctgttg ataggcaaga
     3601 tcggttccgc aatgtgctgg tccacggaag gagtgccggc cgatcttcaa cgtagcggca
     3661 gttgcacgca cgggcatacc gctgctgtac tgtgagttcc gttgtcctca tgccatcgtc
     3721 tataatagat gccgctttct gaatatcact ggtgagcgat gatgcagcat ggcaactcaa
     3781 agaacaattc cgtggttcag tgcaattctc atcgtggctg ttggttccac agcgtcgtca
     3841 tcgacccaaa cgggcggggt ttcgccggat ctggtagaca gggtgtccaa ggccgtagtg
     3901 ctcatcaaag gaacaggcga gaatgggacg atcaccggaa gcgggtttct cgtttcttcg
     3961 gatggcaagc tggtaacaaa tctccacgtt atacgagaaa tgaagcgtgg tggtgtccag
     4021 cttcattcag gtgagacctt tgactccttt tcggtgctgg ccttcgacga gcggagggac
     4081 ttgacgattc tacagattgc tggattcgac ttaccgacga ttgaactcgg caattcaaat
     4141 gacgcaaaaa cgggtgaggc ggttatcgca atcggtagcc cccgtgggct gaacggaaca
     4201 gtaactgctg gcgtcattag tgcaataaga gatgatgcgt caacgggttt caagattatc
     4261 cagacagacg ccgcagccaa tcccggcaat agtggtgggc cgctcattaa cgtccgaggg
     4321 caagtgattg cggttgttac ctccaagttg agggcgtcag aaggtctgaa ttttgccgtg
     4381 ccagtgaact acgtccgagg tctcctaaac attgcaaata cccccctgac tcttgacgca
     4441 ctccgggcag ctttagcggg aggcgggacg gacattttta ggcaggagac tgcgttcccg
     4501 acaagttgga aatcgctaat cagtgggaat gtctacaaca tccgacgaca gagcgacttg
     4561 atctatgtcg agcgcctgtt tccccccgag gcggtaaaag tagggttctt tagtgcttgg
     4621 gaactacgaa aaaaggagca acaatacgta ggcagcatac gtagcgtagt ggtatgcgag
     4681 taccagccgt ggatcgaaac gattgttaaa cgatgcccat tcgagttccg tgccgagttc
     4741 tcactattca cggattcacg catcgaaggg cgaacattag ctccccggaa cggggcgaag
     4801 ctgaattgca ggaaatgtga atacaacaag cctccggttt ggcaatcctt tacgtggata
     4861 ccacagtgac cgcaaatccg taggctccaa cccgacatcc tgatttccac cttggcagtt
     4921 cgtgaaggag gtctgacacg gatgccatat cgaggggcag aacgttgcga cgcttgatca
     4981 cacgtcccgg aagttggaga atcgagacgt tgtggctatt caccttgaac ttgcggcatt
     5041 atccgtggaa gcgatagagg aatacctgct tacggcggaa atgtgcttga agtacaggaa
     5101 accggatggc ggatgcttgg gctatccggc gactctgctt cttttctgcg tcgttgacgc
     5161 cctcggcggt tatctcagta atgacaagtc gaatgagatt ccgaagcacg agccgttcct
     5221 tgttcttcgc catccctgct tcggcctgac agacctcacg gtcgagcaga tcaagaagct
     5281 cgaatggtgg tatcggaatg gccttgccca caatgcagtc cttccacccg gaacatgcct
     5341 gagtggtgaa gacggcgatc ccttcgagtt tgcaccgaac agcgatccct tcaaaatccg
     5401 tgttttctca tttcacagat tggtcagtga cgcatggaga aggtttgata ggacgctgat
     5461 tgttccttca aagattctgc acgaccgaaa aatgcctact gtcggtttca atcctgccga
     5521 tgaaacggga cctataatcg catcgacggg atgtccgcta ccgaagattt gagttgccta
     5581 tatagttgca tggacaacaa aaggtgtttg gggatcagtt gtcccatgca aatcggagaa
     5641 ttcgtttctc gaacgaagaa cggaggaagt ggggagagca aaagttgtta ccgtcgccag
     5701 cgaagtttga tttcttgaac acttacccgg aatggcgcga ctcaggcttt gacggactgc
     5761 ggctcgagca ctactactgg ctggaaatac agacgtgatc gaatgcgccg gattaaactg
     5821 tggagatggc tgatatcgtc aaatcgtctg cctctaccag ctactaacgc aaaatcatct
     5881 acgatgcaca ccgcaaccgg gcagacgttt ggatgcgaaa tgcctacggc gaaatctacc
     5941 tcgagttcag cttggaagat gtggcagcac tccgggagtt tgaagagcgt gcgatggcgc
     6001 tggcttgacg atcagggccg aatggtgaag aatcagttgg gtgaatattc aggccgatga
     6061 atatccaccc gcaacaacac ggattttagc tgattacccg ctggcacgag tctgtacggc
     6121 tcttaaaatt ccaggcgcgc accaagctgg ccgacgcggg gaccggccag tccgttcaaa
     6181 gacgtgatcg tgcccacgtt tgccgcccgg atatttgtcg ccggcgtgct gtagttcgga
     6241 tggttgaaaa cgttgcggaa cgtgccttcc accatcaggc ggacccgttc ggtaagctga
     6301 aattccttac gcagtccgcc gctcacgtcg aaagtgcccg ggcccaccag gatgttcggc
     6361 gccgagtttc caaagcggcc cacgtttacg tccgggcgcc cgtcgccgtc catgtcgccg
     6421 ggcacaacga acgctgtgga gtcgaaccat cgttgcagcg cccgctccga actgggcagg
     6481 tttccgtccg cgagacggtc cggccgcccg ctggttacat tcgtgttgga cacgtcgaac
     6541 ccattgaacg tggggttgaa gaacgagccg gtttgcgcgg ccagcgtgga actcagcgtc
     6601 catccgccga gcacccactg cgcgacgccg ggaaggctgt tcgcgaagcg gcgtccccgt
     6661 ccgaacggaa gatcccagag caggctgccc accaggcggt gccggggctg gtagctcacg
     6721 ttgccatact cgctatgccg ggcgtaggag ttctcgactc cgcagccctg ctcgctgtcg
     6781 ctttgacaat ccgtgagcga cttcgcccag gacca
//
LOCUS       DASTMK010000193         6043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_457235, whole genome shotgun sequence.
ACCESSION   DASTMK010000193 DASTMK010000000
VERSION     DASTMK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457235"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /gene="sufB"
                     /locus_tag="VFQ79_08385"
     CDS             complement(<1..1167)
                     /gene="sufB"
                     /locus_tag="VFQ79_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU0139713.1"
                     /translation="MSTNLDIVQDLANSEYKWGFVTDIKEDKVPKGLNEDIIRLISAK
                     KNEPEFMLDWRLKAYRHWTSLEKAQAEPKWANIKYSPINYQDIHYYSAPMKKTGLTLD
                     EVDPQILRTYEKLGIPLQEQKMLAGVAVDAVFDSVSVATTFKEKLAKEGIIFCSFSDA
                     VQNHPHLVQKYLGSVVPYADNFFAALNAAVFSDGSFVYVPKGVRCPMELSTYFRINAS
                     ETGQFERTLIVADEGAYVSYLEGCTAPIRDQNQLHAAVVELVALDNAQIKYSTVQNWY
                     PGDQDGKGGIYNFVTKRGKCAGVNSKISWTQVETGSAITWKYPSCILQGDNSVGEFYS
                     IALTNNYQQADTGAKMIHIGKNTKSTIVSKGISAGHGQNTYRGLVKILKNAVNAR"
     gene            complement(1374..2648)
                     /locus_tag="VFQ79_08390"
     CDS             complement(1374..2648)
                     /locus_tag="VFQ79_08390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU0139714.1"
                     /translation="MATEAMISTPASATATKPESAAAAWRRTIPETYTVRFSNGAEET
                     YGEGLPAFTLIVRDRRQMLDLFSSDTYSAALSFIRGDFNVEGDLVAAVRFQLNRHRRG
                     WRDLLYAAAARLAPHRIETWLQTRARAAENIRYHYDRSNEFYKQFLDSRMVYSCAYFQ
                     TPGASLEEAQVAKLDHICRKLDLRPGDRFLDIGCGWGSLVIHAAGRYGAQAIGCTLSR
                     NQTEYARESARRVTGAEVAIHEMDFREMSGRFDKIASVGMFEHVGRHRLRSYFRKVHD
                     LLEPDGLFLNHGLVRPEFVTDCAQTLFLQRRVFPGGELAHLSDVIRTAELAGFEILDV
                     EDLRPHYALTCRAWVGRLQRNADACLDAVDRETYRTWLLYLAGSAASFEDGLTGVSQL
                     LLAKRSSPARRLTREYMCAGANGPARAPESIS"
     gene            complement(2693..3430)
                     /locus_tag="VFQ79_08395"
     CDS             complement(2693..3430)
                     /locus_tag="VFQ79_08395"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0139715.1"
                     /translation="MPFRILAVFAHPDDETFLAGGTLAKYAAEGHDVFVVSATRGEAG
                     KRGEYEGLPVEEFALVREREMQAACRVLGLNEPVFLDCADKQLARTCWDSATNEVVRY
                     IRKLKPHVVITFGPDGVSGHPDHIAISQIVTSAVWGAGNRTLISAGEPSRPSSLYYVL
                     RSESVPSCCKPSAPVEAPPATTVIDINGFGERKLEAIRCYRSQNHLRPEDASRLSAVL
                     HAPEHFHRVLPACSSKQIEDHLFVPFL"
     gene            complement(3436..3951)
                     /locus_tag="VFQ79_08400"
     CDS             complement(3436..3951)
                     /locus_tag="VFQ79_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139716.1"
                     /translation="MPAWTGEHIEVDLLGGNQSLPEIDIENRIEHVTILAALPLKEDQ
                     IALRAILGGQPWDLQFARSWSEVHSALRVERVDSVTIVLTEARCPGGGSWKDILGEIE
                     GKANPPALIVADRLADERLWAEVLNLGGYDVLAKPFDAAEVLWVIGSALRLSRRGVKS
                     ASAPAAQTIEA"
     gene            complement(4056..4364)
                     /locus_tag="VFQ79_08405"
     CDS             complement(4056..4364)
                     /locus_tag="VFQ79_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU0139717.1"
                     /translation="MAYVIAEPCIGVKDTACVDACPVDCIHPKKNTTYEDGRPGFEQV
                     EKLYIDPVECIDCGACVPVCPVSAIFALEDLPEKWRHFAEIDASYVVGGKFRPDKFKK
                     "
     gene            complement(4434..4883)
                     /locus_tag="VFQ79_08410"
     CDS             complement(4434..4883)
                     /locus_tag="VFQ79_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0139718.1"
                     /translation="MIKHPGDPVLDIGTGDCACVASILASQGTQVVAVDKDRETIHAA
                     RGFLSTQPMKKAVRLLQDDITASGLASSSFQNIVCFNVLHHVSRFDSGLGELHRILTS
                     DGRLIISEYDENRDGFLKRLEQAVHRRFRGVTAYRQPKGRLVLVCEK"
     gene            complement(5109..5402)
                     /locus_tag="VFQ79_08415"
     CDS             complement(5109..5402)
                     /locus_tag="VFQ79_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU0139719.1"
                     /translation="MVTRIQVLSNLKEVVDPEIGANLVDLGLIRDVLIDDNRIQVRMV
                     LTIPGCPLARYLISQVENKVQAVAEGRTVDVQLLDEPWDPASMFWRDAGDLER"
     gene            complement(5416..5646)
                     /locus_tag="VFQ79_08420"
     CDS             complement(5416..5646)
                     /locus_tag="VFQ79_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139720.1"
                     /translation="MTEWEVLARGENGIGVNRCPEGHIHVELEQGELTLRFDEARFLA
                     FARIVAIAAAAVAGHEPVSPAAKRPEGRQWRN"
     gene            complement(5660..>6043)
                     /locus_tag="VFQ79_08425"
     CDS             complement(5660..>6043)
                     /locus_tag="VFQ79_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEU0139721.1"
                     /translation="VEHIGLATAADLLLIAPATANIIGKLASGIADDMLSTTVLAVRA
                     PLLIAPAMNERMWLNPMVQANVERLKNFGAHFVNPEYGPMACGGEGWGRLARLEEIVR
                     HASEVIRDEVRTAPDRSPEELVAQA"
BASE COUNT         1252 a         1861 c         1666 g         1264 t
ORIGIN      
        1 gcgcgcattg accgcattct tcaggatctt caccaggccg cggtaggtgt tctggccatg
       61 cccggcggaa atacccttgg agacgatcgt gctcttcgta ttcttcccga tgtggatcat
      121 cttagcgccg gtatcggcct gctggtagtt gttggtcagc gcgatcgagt agaattcgcc
      181 caccgaattg tcgccctgca ggatacagct gggatacttc caggtaatgg cggaacccgt
      241 ctcaacctgc gtccaggaga tctttgagtt cacaccggcg cacttgccgc gcttggtcac
      301 gaaattgtaa atgccaccct tgccgtcttg atcgcccgga taccaattct gcacggtgga
      361 gtacttgatc tgcgcattgt cgagcgcgac taactcgact acggccgcgt gcaactgatt
      421 ctgatcgcga atcggggcgg tgcagccctc gaggtagctg acgtaagcgc cttcgtcggc
      481 aacgatcagc gtgcgttcga actgcccggt ctcggaagcg ttaatgcgga agtaggtgga
      541 cagctccatc ggacagcgga cacccttcgg gacatagacg aaggagccgt cgctgaacac
      601 ggccgcattc agcgctgcaa aaaagttgtc agcatacggc accaccgaac ccaggtattt
      661 ctgcaccagg tgaggatggt tctgcactgc gtcggagaag gagcaaaaaa tgatgccctc
      721 tttcgccagc ttttccttga acgttgtggc cactgaaacg ctgtcgaaca cagcatccac
      781 cgctacgccg gccagcatct tctgctcctg aagcggtatg cccagcttct cgtaggtgcg
      841 caggatctgc gggtcaacct cgtccagggt caggcccgtc ttcttcatgg gcgccgagta
      901 atagtgaatg tcctggtaat tgataggaga gtacttgatg ttggcccact ttggctcggc
      961 ttgggccttt tcgagcgacg tccaatggcg gtatgccttc agacgccaat ccagcatgaa
     1021 ctccggctcg ttcttcttcg ccgagatcag tcgaatgatg tcctcgttga gccccttggg
     1081 cactttgtct tctttaatgt cggtgacaaa gccccatttg tactcggagt tggcgagatc
     1141 ctgaacaata tcgagattcg tgctcactgc gttctcccgg aatccggacc cgctgtgatg
     1201 atgagaccct ttatcatgtc ctttcctcca ggcgggaact agcaacagtt tcaatgtgcc
     1261 tctcgtccgc cacttcgagg cgagtcccta accgcaacaa gctgggcaac aacagtccgc
     1321 ctccgtcatc tgcatcatct tcattaagcg agcggtccgg ccagaagcgc gagttaagaa
     1381 atcgactcgg gcgctctcgc cgggccattc gccccagcgc acatatactc gcgggtcagg
     1441 cgcctggccg gactgctccg tttggctagc agcagttgag aaacgccagt gagcccgtct
     1501 tcaaaactgg ccgcggatcc cgccaagtac agcagccagg ttcgataggt ctcccggtcc
     1561 acggcatcga ggcatgcgtc cgcgttccgc tgcaggcgcc cgacccacgc gcggcaagtg
     1621 agcgcgtagt gtggccgcag atcctccacg tccaggattt cgaacccggc cagttcggcg
     1681 gttcgaatca cgtcggatag atgcgccagt tctcccccag ggaataccct tcgttgcagg
     1741 aaaagggtct gcgcgcagtc tgtcacgaat tccggacgaa cgaggccgtg gttcaagaaa
     1801 agcccgtctg gctccagcag gtcgtgcact ttgcggaagt agctgcgcaa gcgatggcgg
     1861 ccgacgtgtt cgaacattcc aacggaggcg atcttgtcaa acctgccgga catttcgcga
     1921 aagtccattt cgtggatggc cacttccgct ccggtgaccc gtctcgcaga ctccctcgcg
     1981 tactcggttt ggtttcgact cagagtgcag ccgatcgcct gcgctccgta gcgtccggcc
     2041 gcgtgaatca ccagcgatcc ccacccgcaa ccaatatcga ggaatcggtc gccgggccgg
     2101 agatcgagct tccggcaaat atggtccagt ttcgcgactt gcgcttcctc gagtgacgcc
     2161 cctggcgtct ggaagtaggc gcacgagtaa accatgcgcg aatcgaggaa ctgcttgtag
     2221 aactcattcg accggtcata gtggtagcga atgttctcgg cggcgcgcgc ccgcgtctga
     2281 agccaggttt cgatgcgatg cggagccagg cgcgctgccg ctgcatacag aaggtcccgc
     2341 caaccccggc gatgtctgtt cagttgaaac cgcactgctg cgacgaggtc accctcaaca
     2401 ttgaagtcgc cgcgaatgaa cgagagcgcc gcggagtagg tatcggaact gaacaggtcc
     2461 agcatttgtc gccgatctcg aacgatcagt gtaaaagcgg gaagaccctc cccgtacgtc
     2521 tcctcggccc cgttagaaaa acgcaccgtg tatgtttcag ggatcgtcct gcgccacgcc
     2581 gcagcggcag actcgggctt ggtcgctgta gcggaagcgg gggtggaaat catcgcttct
     2641 gttgccatcg gctctccttt tctatgacct caacgttgaa tagcacctca attcacagga
     2701 acggcacaaa cagatgatct tcgatttgtt tacttgaaca ggccgggagc acgcggtgaa
     2761 aatgctcggg cgcgtgtagc acggcgctca gtcgcgaagc gtcttcaggc cgcaagtggt
     2821 tttggcttcg ataacagcga atcgcctcca gtttgcgttc cccaaagccg tttatgtcga
     2881 tcacggtcgt tgctggcggc gcctcgaccg gagcggaagg cttgcagcac gagggaacgg
     2941 actccgatcg cagcacatag taaagagaag aaggccgcga cggttcacca gccgagatca
     3001 acgttcgatt gccggcgccc caaactgcgg aggtcacgat ctggctgatt gcgatgtgat
     3061 cgggatgtcc cgaaacccca tccggaccga acgtgatgac aacgtgcggc ttcaatttgc
     3121 ggatatatcg gacgacttcg ttcgttgccg aatcccagca ggtgcgggcg agctgtttgt
     3181 ctgcgcagtc gaggaacacc ggctcgttca acccgagtac acggcaagcg gcctgcatct
     3241 cacgctcccg aactagggcg aactcttcga cagggagacc ttcgtattcg ccgcgcttgc
     3301 cggcttcgcc acgcgtcgcg gaaacgacaa aaacatcgtg gccttcagcc gcgtacttcg
     3361 ccaacgttcc accggcgaga aatgtctcgt cgtcagggtg cgcgaaaaca gccagaattc
     3421 ggaatggcat attcttcaag cctcaatcgt ctgagctgct ggggctgacg cagacttcac
     3481 gccgcgtcgc gaaaggcgca aagccgaacc aatgacccaa aggacttcag cggcatcgaa
     3541 aggtttcgcc agcacgtcat agccgccaag gtttagcact tccgcccaca accgttcgtc
     3601 ggccaaccga tccgccacaa tcaaagctgg tggattggct ttgccctcaa tttcgcccag
     3661 aatatctttc caagaaccac cgccgggaca gcgcgcttcg gttagcacaa tagtgaccga
     3721 gtccacacgc tcgacgcgga gagcggagtg gacctcagac caactacgcg caaactgcaa
     3781 gtcccagggt tgaccaccca ggatcgcccg aagcgcgatc tgatcctctt tcaaaggaag
     3841 ggcggcgaga atcgtcacat gctcgatgcg gttttcgata tctatttccg gcagactctg
     3901 atttccaccg agcaggtcaa cctcaatatg ctccccggtc caggcgggca cggctcgatg
     3961 agagtttcca cttcccctga ggaccatttc ggtgtcggct ggttcctgac cacctgtcca
     4021 gagtccagtt cgcgagtttg tacctgcgca gagtctcatt tcttgaattt atcgggtcgg
     4081 aatttcccgc caacaacgta actggcatcg atctcggcaa agtgacgcca tttttcgggc
     4141 agatcttcga gagcaaaaat cgctgaaacc gggcagactg gaacacatgc accacagtcg
     4201 atgcactcaa cagggtcgat gtagagtttt tctacctgct caaagcccgg ccggccgtcc
     4261 tcataggtgg tgttcttctt cggatgaatg caatccaccg gacatgcgtc cacacaggca
     4321 gtatctttca caccgatgca cggttcagca atcacatagg ccacatgcca tctcctctcg
     4381 gccggggccg ccacggctcc caacaaacgc ggctcataaa tccgttcccg ccttcatttt
     4441 tcgcagacca ataccaagcg ccctttcggc tggcggtagg cagtgacacc cctgaaacgc
     4501 cggtgcactg cttgttccaa tcgcttcaag aaaccatccc gattctcgtc gtattcgctg
     4561 atgatcagcc ggccgtcgga agtcagaatt cggtggagtt cgcctaagcc gctgtcgaag
     4621 cgcgaaacgt gatgcaggac attgaaacac acgatgttct gaaaggagct ggaagccaga
     4681 cccgaggcgg tgatgtcatc ctgcaacagc cgaacagcct tcttcatggg ctgcgtactt
     4741 agaaaccctc gggcggcgtg tattgtctca cggtccttgt cgacagcgac cacctgcgtg
     4801 ccctggctgg cgaggatact ggcgacgcag gcgcaatccc cggtgccgat atccagcaca
     4861 ggatcgcccg gatgctttat caacgacagg gcaaagctcc gtgcgtcttg cgacccggtt
     4921 ttcgcggggg cgcatttaag tggcgctgac gccacacttt cttccgcaca cgcgtgcggt
     4981 tcagctatca cgttcgccat aagccaaccc ctctcgggcc acaccgcgga cggcttcaat
     5041 gtccgcggtg cctcggaccg gaaacgtatc gttttcaggg tgtgcgaaca tcccgagtaa
     5101 ccgagatctc atctctccag gtcccccgca tcacgccaaa acatcgaagc ggggtcccat
     5161 ggctcatcga gtaattgaac gtccaccgtc cttccctcgg caaccgcctg gaccttgttc
     5221 tcaacctgcg aaataaggta tctagccaga ggacagccgg ggatggtcaa taccatccgc
     5281 acctggatcc ggttgtcgtc aatgagcaca tcccgtatca agccgagatc caccaaattg
     5341 gcgccgatct ccggatctac aacttccttc aggttggaca gcacctgtat tctggtaacc
     5401 accgtgcgcc tcctatcaat tgcgccactg ccgcccctca ggccttttcg cggcggggct
     5461 caccggctca tggccggcga cggcggcggc ggcgatggcg acaatgcgag caaatgccag
     5521 gaagcgggcc tcgtcgaagc gaagggtgag ttctccctgt tcgagctcga cgtggatgtg
     5581 accttccggg caacggttga ctcctatccc gttttcaccg cgggccagga cttcccattc
     5641 cgtcatgatg gacctcctat cacgcctgtg cgacaagctc ttcgggacta cggtcaggcg
     5701 cggttcgaac ctcgtccctg attacctcag aggcatggcg cacgatttct tccagtcgcg
     5761 cgaggcggcc ccacccctcc ccgccacacg ccatcggccc gtactccgga ttcacaaaat
     5821 gcgctccaaa gttctttagc cgctcgacgt tagcctgtac catcggattt agccacatcc
     5881 gttcattcat ggctggtgcg atcagcagcg gagccctcac ggcgagaact gtcgtcgaaa
     5941 gcatatcgtc ggcgatcccg ctggcgagct ttccgataat attcgccgtc gccggggcga
     6001 tcaggagcag atcagccgcg gtcgccagtc caatatgctc cac
//
LOCUS       DASTMK010000194         4829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_585849, whole genome shotgun sequence.
ACCESSION   DASTMK010000194 DASTMK010000000
VERSION     DASTMK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585849"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..559
                     /locus_tag="VFQ79_08430"
     CDS             14..559
                     /locus_tag="VFQ79_08430"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEU0139722.1"
                     /translation="MNLKRTLMIALGSAGFLLAQDTAVVRPSIYPPPDREPARALKEY
                     LNLTEAQAQALQNVQKSRQEAEQVIYEQMRQKQLALDSLLQSGSTDYTQIGRLLAEIR
                     DLQKKLPISGEPYRTQALSVLSQEQRLKLPALVDVLKLQTPANDAVFWNLIDRPEQEP
                     EPPIILGGREAADVHILPARK"
     gene            679..1191
                     /locus_tag="VFQ79_08435"
     CDS             679..1191
                     /locus_tag="VFQ79_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0139723.1"
                     /translation="MDEARIVSLHAQVEQLYEDARQDVYYYLLTFGIDPGQAQDLSQE
                     TFLRLYMALREGQQIRNERAWIFRVAHNLGLNARASQKKIRSIGTDSELPLPVIAENP
                     ERNLLEAERRSLLDRAIAELSPQQRQCLLLRVEGLRYREIAGIVGISISTVAEFLRRA
                     VSKLKRVAHE"
     gene            1184..2821
                     /locus_tag="VFQ79_08440"
     CDS             1184..2821
                     /locus_tag="VFQ79_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0139724.1"
                     /translation="MSDASCSHIEDTELLAYADGELDARQAADIRRHLADCPECELRL
                     RRLRAGLEDYTWRIEEDKRRVSPPRPWQNLEPEFSRIDREAAARLRRAAPRTHRWLAV
                     AATVLVAVIIYQFSRENSVSAAELLDKATQTGVATEPGRRIRMKTRQNALVRPARLTA
                     TTPQRAVNDSSFIQIKALFEQARFSWEDPLSARAFAEWRNRLEEKSDEVTVLGSGNQR
                     AERQYRIRTTTPEGVLAEASLTLRANDLRPLHETLRFRNNEWVEIVEAEPAGDMPSRQ
                     MEPPPPIASSRESISPESVEETPAGADEELHVIAALNRIGADLGEPLEVRRETAERRI
                     LVTGIGIDPARQKQILGLVASIPGVSVQFEDPHPVESLPSEGVQAPVAAGPKPPLHDL
                     LKEKLGAGRPVEQFVDRVLALSDSALSRAHALHSLSERFPPEVERAFEAPDRSLLGSI
                     RLQHSRALLSIADQLERELDTLLSGTVRERAPPGQCGTWQQCAAGILRTTQELDETLN
                     LALAGTQPEPPDQLTSKLGETLDRWLQAVRTSGTGSR"
     gene            2818..3267
                     /locus_tag="VFQ79_08445"
     CDS             2818..3267
                     /locus_tag="VFQ79_08445"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0139725.1"
                     /translation="MKAVLTFLLFAAFAAQAQQRAQLTGVIRDSSGLGVPDTDVVMLN
                     VETGIRRSTRTGEYGSYAVSSLAPGEYKVTVRKSGFRTIARTGIRLGATERARLDFLL
                     EIGGMHEVVTVEGSASPIDTNDATSGFCQASRSGVSSDLAYCLGSKR"
     gene            complement(3404..>4829)
                     /locus_tag="VFQ79_08450"
     CDS             complement(3404..>4829)
                     /locus_tag="VFQ79_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139726.1"
                     /translation="AYGLGLASMYTQQFGDPVADAGKTVLGVFFQDAWRIAPRLTLNL
                     GARYDVEWADSLTPGSDAMRGVFERLSLRRSPPVDRNNIQPRAGFAYQVLRDGKLTVR
                     GSYGVFYDRLLNLTVYMGAASDGVQITRVTLPGAAAAAVFQTPAQKFAEFPASGGSTG
                     LTAFSNNWRLGNSQQANFTLSSQIRPGVTLDAAYVWVKGTHLARSRDYNPTDSQQAAA
                     FLGAGNSPATLLARNYFRPEPAVSEAMVVEGSASSAYNGLRLGIRGEVSSSLSVNASY
                     TFSKAIDDAEEIFPHTRAQDMRNFRAERGLALYDQRHRLVFAGIYRLRRPATTPLSRL
                     WNNWSVAPHLELGSGRPVNVLLGSDNNLDQDPGSNRPDLVPAGAANSYSTRYGAFAAP
                     VLGRAGNLGRNAFAGPGYASLNLRVQRDFRISETLDCQFIAESFNLFNRTNVRSVNPN
                     YQSAGEPLSAFDPRQIQVGVRLRF"
BASE COUNT         1015 a         1427 c         1492 g          895 t
ORIGIN      
        1 atcagaaaaa ataatgaatc tgaaacgcac gctgatgatc gctcttggca gcgccggttt
       61 ccttctggcg caggacacgg cagtggtcag acccagtatt tatccacccc ctgatagaga
      121 acccgccagg gcattgaagg agtatctgaa tctcacggag gcacaggctc aggctctaca
      181 gaatgtccag aagagccggc aggaagccga gcaggtcatc tatgaacaga tgcggcaaaa
      241 gcaactggcg ctcgacagtc tcttgcaatc cggctcgacg gactataccc agatcgggcg
      301 cctgctggct gaaatccgcg atcttcaaaa gaaactgcct atttccggcg agccgtaccg
      361 gacccaggcg ctatcggtgc tttctcagga gcagcgtctc aaattaccgg ctctcgtgga
      421 tgttctgaag ttgcaaacac cagccaatga tgcagtcttt tggaatctta tcgaccggcc
      481 ggaacaggag ccggaacccc caattattct cggcgggcgg gaagcggcgg acgttcacat
      541 cctgcccgcg agaaaatagt tgataactct gcgatcggag gagcgaaagg gggcgcggca
      601 gccagtccgc cctctctcga ctggctcttg ctgactttgc gagacacaac ccatgacgcg
      661 cggtccggaa aatcccgttt ggacgaggcg cgcattgtct cgctgcatgc gcaggtcgag
      721 cagctttatg aagatgccag acaggatgtc tactactacc tgctcacatt cggcattgat
      781 ccgggccagg cacaagacct gtcacaagag acattcctcc ggttgtacat ggcgcttcgg
      841 gaaggacagc aaataaggaa cgaacgggca tggatattcc gggtggcaca caatcttggg
      901 ttgaacgccc gtgcgagtca gaaaaaaatc cggtctatcg ggacagactc cgaattgccg
      961 ttgccggtta tcgcggaaaa tccggaacgg aatcttctcg aagcggaacg gcgctcgcta
     1021 ttagatcgcg ccattgcgga attgtcaccg cagcagcgcc agtgtctgct gctccgcgtg
     1081 gagggtttgc ggtatcgcga aatcgccggc atcgttggga tcagtatctc caccgtcgcg
     1141 gagtttctgc gccgcgccgt gtcgaaactg aagagggttg cccatgagtg atgcgagttg
     1201 ctcccatatc gaagacacgg aactgctggc ctatgccgac ggcgaactgg acgcccggca
     1261 ggctgccgac atccggcggc acctggcgga ttgtccggaa tgcgagctgc ggctgcggcg
     1321 tttgcgtgcc ggtttggagg attacacgtg gcggatcgag gaggacaagc gaagagtatc
     1381 gccgccacgg ccgtggcaaa atctggagcc ggagttcagc cgcatcgatc gcgaagccgc
     1441 agcccggttg cggcgggcgg cgccgcggac ccaccgctgg ctcgcggttg cggcaacagt
     1501 gcttgtggcc gtgattattt accagttctc aagggaaaac agcgtcagcg ccgcggaact
     1561 gctggacaaa gccacgcaaa ccggggtcgc cacggagccg ggccggcgca tccggatgaa
     1621 gacacgccag aacgctctcg tccgtcccgc aaggctcacc gctacaactc cgcagcgcgc
     1681 cgtgaatgat agctcattca tccagatcaa ggcgttattc gaacaagcga ggttcagttg
     1741 ggaagacccg ctcagcgccc gcgcatttgc cgaatggcgg aaccgtttgg aagaaaaaag
     1801 cgatgaagta acggtattag gcagtgggaa ccagcgtgcc gaacggcaat accgcatccg
     1861 gaccaccacc ccggaagggg ttctggcgga ggcatcgctt acgttgaggg cgaacgacct
     1921 gcggccgctg cacgagactc tccgcttccg aaacaacgaa tgggtggaga tcgtggaggc
     1981 cgaaccagcc ggagacatgc catccaggca gatggaacca ccgcctccga tcgcttcaag
     2041 ccgtgagagc atctctccgg aatccgttga agagacaccg gcaggtgccg atgaggagct
     2101 gcatgtcatc gctgccttga atcgcattgg cgcggatctc ggagagccgc tggaggtgag
     2161 gcgggagact gcagaacgcc gtatcctggt cacgggaatc ggcatcgacc cggcacgcca
     2221 aaaacagatt ctcggattgg tggcgagcat cccgggtgtg tcggtgcagt tcgaagatcc
     2281 gcatccagtg gagtctctcc ccagcgaggg cgtccaggcg ccggtggcgg cgggcccgaa
     2341 gccaccactt catgatcttc tgaaggaaaa actgggcgcc ggaagaccgg tcgaacagtt
     2401 tgtcgatcgg gtcctggcgt tgagcgattc ggccctctct cgcgcacacg ctttgcacag
     2461 cctctccgag aggttcccac cggaagtcga gcgggcattc gaggccccgg accgcagcct
     2521 gctcgggagt atccgcctgc aacatagccg cgctctcctc tcgattgcgg accagttgga
     2581 gcgcgaactg gacacacttc tttccggcac cgtgcgggag cgtgcaccgc ccgggcagtg
     2641 cggcacgtgg cagcaatgtg cggcaggcat actgcgcaca acccaggaac tggatgagac
     2701 gctcaatctc gcacttgcag gcacgcagcc ggagccgccg gatcagctta cgtcgaagtt
     2761 gggtgaaacg ctcgatcgct ggttgcaagc ggttcgcacc tcgggcacgg gttcccgatg
     2821 aaagccgtat tgaccttcct gttgttcgcc gcatttgctg cgcaagcgca gcagcgtgcg
     2881 cagttgacgg gagtgatccg tgattcctcg ggactcggtg tccccgatac cgatgtcgtg
     2941 atgctgaacg tggaaacagg catccgtcgt tcgacccgga cgggtgagta cggatcttat
     3001 gcggtctcct cgctcgcacc aggggaatac aaggtcacag tccgaaaaag tggatttcgc
     3061 accatcgcac gaacgggaat ccggctcggc gcaacggagc gggcacggct cgactttctg
     3121 ctggagatcg gtgggatgca tgaagtggtt acggtggagg gcagcgcctc gccgatagac
     3181 acgaacgatg caacttccgg cttctgccag gcgtcccgct ccggagtgtc aagcgacttg
     3241 gcatactgct tggggtcgaa gcggtgatcc atgtggggcc gctcaggagc ggattgagcg
     3301 gcgagcaccc cggccgccgc caacagcgca aaaaataagc gggttcggta catttcacgt
     3361 ctctccgttt gatttggatt cggcgctctt ggagtcgttc cacctaaaag cgaagacgca
     3421 cgcctacctg aatctggcgc ggatcaaacg cggacagcgg ttcgccggcg ctctggtaat
     3481 tcgggttgac gctcctgacg ttggtgcggt tgaaaaggtt gaagctttcc gcaatgaact
     3541 gacaatcgag cgtctcactg atgcggaaat ccctttgaac gcgcaggttg agcgatgcgt
     3601 atcccgggcc ggcgaacgcg ttccttccaa ggttgccggc cctgcctaac acgggcgcgg
     3661 cgaaagctcc gtagcgcgtt gagtagctgt tcgcggcgcc ggctggaacc agatcgggcc
     3721 ggttgcttcc cggatcctga tcaagattat tgtcgctccc gagaagcaca tttacgggcc
     3781 ggccgctgcc cagttccagg tgcggcgcca ccgaccagtt gttccacagc cgggacaagg
     3841 gggttgttgc aggccggcgc aatcgataga tgcccgcgaa cacaagccgg tggcgctggt
     3901 cgtacagcgc tagcccacgc tcggcgcgga aattgcgcat gtcctgcgcc cgcgtatggg
     3961 ggaagatctc ctcggcatcg tcaatcgcct tggaaaaagt gtacgacgca ttcacgctca
     4021 gagaagaact cacttcgcct cgaatcccga gtctcaggcc gttgtaggcg ctcgacgccg
     4081 atccctcgac taccatcgcc tcggacactg ctggctcggg ccgaaagtaa ttccgagcca
     4141 gcagcgtcgc cggagaattg cccgccccga gaaaggccgc tgcttgctgg gaatcggttg
     4201 gattgtagtc tcgggacctt gccagatgcg tccctttcac ccagacgtaa gcggcgtcca
     4261 gtgtaacgcc aggccggatc tggctgctca gagtgaaatt cgcctgttgg gaattaccca
     4321 gtcgccagtt gtttgaaaag gcggtcaagc ccgtcgatcc gccactcgcc ggaaattcgg
     4381 cgaacttttg cgccggcgtt tgaaaaacgg cagccgccgc ggcgcccggc agagtgacgc
     4441 gagtgatctg gactccgtcg ctggctgccc ccatatagac cgtcaggttc agcagacgat
     4501 cgtaaaacac tccgtaggaa cctcgaaccg tcaacttgcc gtctctcagg acctggtaag
     4561 cgaagccggc gcgcggctgg atgttattcc ggtctaccgg tggagagcgg cgcaacgaga
     4621 gcctctcaaa tacgccacgc atggcatccg agccgggtgt gagcgaatcc gcccattcca
     4681 cgtcgtaacg cgcccctaga ttcaacgtga gccgcggtgc gattcgccag gcatcctgga
     4741 aaaagacgcc taagacggtc ttgcccgcat ccgccaccgg atctccgaac tgctgggtgt
     4801 acatcgatgc aaggcccagt ccatacgct
//
LOCUS       DASTMK010000195         5608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_169770, whole genome shotgun sequence.
ACCESSION   DASTMK010000195 DASTMK010000000
VERSION     DASTMK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169770"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFQ79_08455"
     CDS             <1..658
                     /locus_tag="VFQ79_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139727.1"
                     /translation="AIAESGLVQPLLASGFAVLAVDLRGCGETLGHFRERWDTNFRLV
                     ANQVLFGRPLAGRRAFDLTRTVDYLTRRRLPLNGLTVTGIGDDALPVLLAAAIDPRIK
                     NIAVSGYFHSFLSQMRTFPRQLPNAWNDPQLRGRFDTGEYEVDFGSVIPSVLRYADIP
                     DLLSLAEPRRILFCDARDRNSIGETLRSRFRTVTNGKNVRYEPAAALDARLLLEWVGK
                     "
     gene            complement(677..973)
                     /locus_tag="VFQ79_08460"
     CDS             complement(677..973)
                     /locus_tag="VFQ79_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0139728.1"
                     /translation="MDYSVAAKWLLPKPGRDMAMRLLEAQKAFRLMEKCAPRLFDMRI
                     GLHRALDLALQYQLSLWGCIYLELAIEHECPFITSDLRLFRGSAARHPLIRLLS"
     gene            complement(996..1235)
                     /locus_tag="VFQ79_08465"
     CDS             complement(996..1235)
                     /locus_tag="VFQ79_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139729.1"
                     /translation="MKNIALKNIPEAVYRAMKREAKQQGRSLNAQIIGVLESAVAELE
                     RRRRLPGLRKKLNRFAVSLPPLEDSAPLIRQDRQR"
     gene            1291..4452
                     /locus_tag="VFQ79_08470"
     CDS             1291..4452
                     /locus_tag="VFQ79_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEU0139730.1"
                     /translation="MQFRLVLLLSGVAVAASGAPPQKPIDFNRDIRPILSDTCFACHG
                     PDEQHRMVGLRLDTKDGTFAERKGGPVIVQGDPVASRLYQRVSAEKKAMRMPPAGAGQ
                     ELTAAQIDLLRQWIEQGAKWETHWAYTPPVRPEPPAVREKGWSRNPIDNFVLARLERE
                     GLEPSREADRATLLRRVTLDLTGLPPTPAEVNAFLPDKSPDAYEKVVDRLLASPRHAE
                     RMAMQWLDLARYADTHGYHIDSHRDMWPWRDWLIRAFARNMPYDEFTIEQLAGDLLPS
                     PTRDQLIATGFNRNHMINFEGGAIPEEYHVEYVVDRVEATSNVWMGMTLGCARCHDHK
                     YDPVSQKDFYRFFAFFNNVSDKGLDGRYGNAQPFLQLSTPEQKSLLDNLTWQIECKES
                     DLDGEQLAPQITEWEKQRRAAVAPDSPKDGLVGHWEFDGHLADTSGKYRHATVPRGEV
                     TWAEGPVVRSAVFSGETHASLGGAADFDSSDTFSLALWIRRSGSNFTVLHKSDPGQDH
                     RGFDISFDEFVPVPDLKRASHLTFRLIHKWPSSAIVLRTRERLIQGDWDHIVINYDGS
                     GKAAGVKLFVNGKPYETDVLEDTLAGSFRTSAPLGIGDKHVARPYKGQIDDLRVYNRQ
                     LTPEEIDHLAIHYPIDTVLASAKRSKDQKSRVREYFLTYAAPENLRAEYRELNRLLKE
                     KKELEKQIPTTMVMDESKPRETFVLGRGDYRNRTEKVTADVPSALPPLPKDAPRNRLG
                     LARWLIDPAHPLTSRVAVNRYWQMYFGTGLVKTTEDFGSQGEPPSHPELLDWLATEFI
                     RTGWDIRAMQRLIVTSATYRQSSKVTPELVEKDPENRLLARMSRFRLPAETVRDNALA
                     ISGLLVEKTGGPSVHPYQPEGVWEAIAYGDVYSAQKYKQDHGESLYRRSMYTFWKRTA
                     PPPALATFDAPDREKCTARRARTNTPLQALVLLNDPTFVESSRKLAEHMMADGGRDAN
                     SRIRYGWKLATARNPESQEVRPLKALFEQEYANYRDDRESALKLTGVGESEASGKFDA
                     AELAAWTTVASVILNLDETITKE"
     gene            4455..>5608
                     /locus_tag="VFQ79_08475"
     CDS             4455..>5608
                     /locus_tag="VFQ79_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0139731.1"
                     /translation="MSIDNEIRLTMTRRHFFSRTATGIGTVALASLLNPKLLAKSGGL
                     PDLPHFTPKAKSVVFLHQSGAPSQMELFDYKPLLQKLHGTELPDSIRRGQRITGMTSG
                     QDSFPVAAPVYKFEQYGQAGAWVSELMPHTAKIVDDISIVKTVNTDAINHDPAITFIQ
                     TGSQQPGRPSLGSWVSYGLGSENEELPAFVVFVSQTSGLNIDQPLFSRLWSSGFLPSS
                     HQGVNFRSAGDPVLYLSDPPGMHRETRRRMLDALEKLNQIRSAEYGDPEIQTRIGQYE
                     MAFRMQTSVPDLMDLSNEPESTFEMYGPESRKPGSYAQHCLLARRLVERGVRFVQLYK
                     RGWDQHNDLPRDIALQSKAVDQPSAALVRDLKQRGLLDQTLVIWGGEFGRT"
BASE COUNT         1194 a         1688 c         1654 g         1072 t
ORIGIN      
        1 cgcgattgcg gaatcgggtc tggtgcagcc gttgctcgcg agcggctttg ccgtgctcgc
       61 ggtggacctg cgcggctgcg gtgagacact cggtcacttc cgggaacgat gggatacgaa
      121 ctttcggttg gtggcgaacc aggttctgtt cggacgtccg ctcgcgggca gacgcgcttt
      181 cgacttgaca cggaccgtcg actatctgac acgaagacgc ctgccactga acggcctcac
      241 ggtcactggt atcggagacg atgcattacc tgtgctgtta gccgccgcca tcgacccgcg
      301 catcaaaaac atagcggttt ccgggtattt tcacagcttc ctctcgcaaa tgagaacatt
      361 ccctaggcaa ctgcccaacg cctggaacga cccgcaacta cgcgggcgct tcgacaccgg
      421 cgaatatgag gtcgacttcg gctctgtgat tccgtcggtt ctccgctacg ccgacatccc
      481 cgatcttctc tctctggcgg aaccgcgacg gatattgttc tgcgacgcga gagaccggaa
      541 ctcgatcggg gaaaccctgc ggtcccggtt ccgcacggtc accaatggaa agaacgtccg
      601 ctacgagccc gctgccgctc tcgacgcccg cctgttgtta gagtgggtgg gtaagtgaag
      661 ggatggtgac agcttctcag ctcaggagac ggatcaacgg atgcctggca gcgctgcctc
      721 tgaacagacg gaggtccgag gtaataaacg gacactcatg ttcgatggca agttccaggt
      781 aaatacaacc ccacaaagac aactgatatt gaagcgcaag atccaaggcc cggtgcaggc
      841 ctatgcgcat gtcgaagaga cgaggtgcac atttttccat aaggcgaaaa gctttctgtg
      901 cttccagcaa acgcatcgcc atgtcgcggc caggtttggg cagcagccat ttagcggcga
      961 cactataatc cacccacaag ttttgccggc atggattacc gttgccggtc ctggcgaatc
     1021 aggggcgcgc tatcttccaa gggcggcagt gacactgcaa aacggttcaa cttcttccgt
     1081 aacccgggca gccgccgccg tcgttccagt tcggccacag cggattccag aactccgata
     1141 atctgggcgt tgagactacg gccctgttgc tttgcctccc gtttcatcgc ccggtagacc
     1201 gcttccggta tgtttttcaa cgcgatgttc ttcaacccac tccgcctctt aatgtgttat
     1261 acatgtttca aatagcagag gaggggatcg ttgcagttcc gcctggtttt gttgctctcc
     1321 ggggttgccg tcgccgccag tggtgctcca ccccagaaac ccatcgactt taatcgcgac
     1381 atccggccga ttctctccga cacgtgcttc gcctgtcacg gacccgatga acaacacagg
     1441 atggtagggc tccgtctcga taccaaagat gggacgtttg cggaacgaaa aggcggtcct
     1501 gtcatcgttc agggcgatcc ggtggccagc cgcctctatc agcgtgtgag cgccgaaaag
     1561 aaggcgatgc ggatgccgcc tgcgggcgcg ggccaggaac tcacggcggc gcagatcgat
     1621 ctgctacgac agtggatcga acagggcgca aaatgggaaa cgcactgggc gtatacgcca
     1681 cctgtccgtc cggaaccacc cgccgtccgt gagaaaggct ggtcacgcaa cccgatcgac
     1741 aattttgtgt tggcccggct cgagcgcgaa ggtcttgaac catcgcgcga agcagataga
     1801 gccacgctgc tgcgccgcgt aacgctcgac ctcacgggat taccccccac gcccgccgaa
     1861 gtcaacgcct tcctgccgga caagtccccc gatgcgtacg aaaaggtcgt ggaccggctg
     1921 cttgcctcgc cgcgccatgc cgaacgaatg gcgatgcaat ggcttgacct ggcgcgctac
     1981 gccgatacgc atggatatca catcgactcc catcgcgata tgtggccctg gcgcgactgg
     2041 ctgatccgcg ccttcgcgcg aaacatgccc tacgacgagt tcaccatcga gcagctcgcc
     2101 ggtgatctgc tgccgtcccc gacacgggat caactgatcg cgacaggttt caatcgcaat
     2161 catatgatca atttcgaggg cggagccatt ccggaggaat accacgtcga gtatgtcgtc
     2221 gaccgcgtcg aggctacttc gaacgtttgg atgggaatga cactcggttg tgcacgctgc
     2281 cacgatcata aatacgatcc cgtcagccag aaggacttct accggttttt cgcatttttc
     2341 aacaacgtat cggacaaagg cctggacggc cgctacggca acgcgcagcc ctttctgcaa
     2401 ctctccactc ccgagcagaa gagcctgctg gacaatctca cctggcagat cgagtgtaaa
     2461 gaatccgatc tcgatggcga acaactggct ccgcagatca ccgaatggga aaagcagcgg
     2521 cgtgcggcgg ttgcgccgga ctcgcctaaa gacggactgg tgggccattg ggaattcgac
     2581 ggccatctcg ccgacacgtc cgggaagtac cggcacgcca cggtgcctcg gggtgaagta
     2641 acgtgggccg agggtccggt agttcgttcc gccgttttca gtggtgaaac gcacgcgagc
     2701 ctgggcggtg ccgcggattt cgacagcagc gacaccttct cgctggcgct atggatccgg
     2761 agatccggct cgaatttcac ggttctccac aaatccgatc cgggccagga ccaccggggt
     2821 tttgacatct ctttcgatga gtttgtgccg gtcccggatc tgaagcgagc atcgcacctt
     2881 accttccgcc tcatccataa atggcccagc tcagcgatcg tgttgcgcac ccgggaacgc
     2941 ctcatacaag gcgattggga ccacattgta atcaactatg acggctcggg taaggcggcc
     3001 ggagtgaaac ttttcgtcaa cggcaagcct tatgagacgg atgtactgga ggacacgctc
     3061 gcgggttcat tccgtacgtc cgcgcccctc ggcatcggcg acaagcacgt tgcacgccct
     3121 tataaaggac agatcgacga tctgcgggtc tacaaccggc aactcactcc ggaggagatc
     3181 gatcaccttg cgattcatta tccgatcgac acggttctcg cttccgctaa gcggtcgaag
     3241 gatcagaaat cacgtgtacg ggaatacttt ctcacctacg ccgcaccgga aaacctgcgc
     3301 gcggaatacc gcgagttgaa ccggctcctc aaagaaaaga aggagctgga aaaacagatc
     3361 ccgaccacga tggtgatgga tgagagcaaa ccgcgcgaaa cgttcgttct tggccgtggg
     3421 gattatcgca accgtacgga gaaggtaact gccgacgtcc cgtccgccct gcccccgctg
     3481 cccaaagacg caccgcggaa ccgcctcggg cttgcacgct ggctgattga tcccgctcac
     3541 ccgctcacct cccgcgtggc ggtgaaccgg tattggcaaa tgtatttcgg caccgggctg
     3601 gtaaagacaa cggaggattt cggatcgcaa ggggaaccgc cgagccatcc cgaactgctt
     3661 gattggcttg ccaccgaatt catccgcacg ggatgggata tccgggccat gcagcggctg
     3721 attgttactt cggcgaccta ccggcagtcg tccaaagtca cacctgagct ggtcgagaaa
     3781 gacccggaga acaggctgct cgcccgcatg tcgcgcttcc ggttgcccgc cgagacggta
     3841 cgcgacaacg cgcttgccat cagcggcctg ttagtcgaga agactggcgg ccccagcgtt
     3901 catccctacc agccggaggg cgtgtgggaa gccatcgcct atggcgatgt ctattcggcg
     3961 cagaaataca aacaggatca cggtgaatcg ctgtaccgcc ggagcatgta tacgttctgg
     4021 aagcgcacgg cgccgccgcc cgcgctcgct acattcgatg cgcccgaccg cgaaaagtgc
     4081 accgcgcgac gggcacgcac gaacacgccg cttcaggcgc tggtgctgct gaacgatccg
     4141 acgtttgtcg agtcgtcgcg caagttggcg gaacacatga tggcagacgg cgggagggac
     4201 gcgaattccc gtattcgata cgggtggaag ctggcgacgg cacgcaaccc ggagtcccag
     4261 gaagtacgcc cgctgaaagc tctgttcgaa caggagtacg cgaactaccg ggacgaccgt
     4321 gagagcgcct taaagctgac cggcgtgggc gagtcggaag cgagcggaaa attcgatgcg
     4381 gccgaactgg cggcgtggac gacggtagcg agcgtcatcc tgaacctgga tgaaacgatc
     4441 acaaaggaat agcgatgagc atcgacaacg aaatccggtt gaccatgacg cgccgccact
     4501 tcttttcgcg gactgcgacg ggaatcggca cagttgcgct ggcgtcgctg ctcaatccga
     4561 agctgttggc taagtcggga ggtctgccgg accttcccca tttcactccg aaggccaaga
     4621 gtgttgtctt cctgcaccaa tcgggagcgc catcacagat ggagcttttc gattacaagc
     4681 cgctgctcca aaagcttcac ggaaccgaac tgccggattc catccgaaga gggcagcgca
     4741 tcaccgggat gacgtcgggg caggattcgt tccccgtcgc cgcgccggta tataagttcg
     4801 aacagtacgg gcaggccggc gcctgggtaa gcgaactgat gccgcacacg gcgaagatcg
     4861 tggacgacat ctcaatcgtc aagaccgtaa acacggatgc catcaatcac gatcccgcta
     4921 ttacgttcat acagaccggc agccagcaac ccgggcgtcc gagcctcggc tcgtgggtca
     4981 gctacggttt gggcagcgag aacgaggagt tgccggcgtt cgtcgtcttc gtatcgcaga
     5041 ccagcggatt gaacatcgac cagccgttgt tttcgcgatt gtggtccagt ggcttcctgc
     5101 cgtcgagcca tcagggcgtc aacttccgct cggccgggga tccggtgctc tatctttccg
     5161 atccgccggg catgcaccgg gaaacgcgcc gcagaatgct ggacgcactc gaaaagctga
     5221 accagattcg ttcggccgaa tacggtgatc cggaaataca gacgcgcatc gggcagtacg
     5281 agatggcctt ccggatgcag acttccgtgc cggatctgat ggatttgtca aacgagcccg
     5341 agagcacgtt cgaaatgtac ggaccggagt cgcgcaagcc aggctcgtat gcgcagcatt
     5401 gtttgctggc acgccgcctg gtggaacgcg gcgtccgttt cgttcagctt tacaaaagag
     5461 ggtgggacca gcacaacgat ctgccccgcg acatcgcgct gcaatcgaaa gcggtagatc
     5521 agccctcggc ggcactggtc cgcgatctga agcaacgagg attgctggac cagacgcttg
     5581 ttatctgggg cggcgagttc ggccgtac
//
LOCUS       DASTMK010000196        21625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_41699, whole genome shotgun sequence.
ACCESSION   DASTMK010000196 DASTMK010000000
VERSION     DASTMK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21625
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41699"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFQ79_08480"
     CDS             <1..420
                     /locus_tag="VFQ79_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139732.1"
                     /translation="VERLRAAAAKAGDRRYAALCQELNLASDSIDDIPDRLLLRRLVE
                     TLEREPNRAQSSNGGDNTAGSEAQTDGAAVLSDLRGRLLQQASRVSGATRRTLAQVIN
                     EAANGAFKFANLKDLNPEDAGKVRDALAALERMAITT"
     gene            1116..1481
                     /locus_tag="VFQ79_08485"
     CDS             1116..1481
                     /locus_tag="VFQ79_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139733.1"
                     /translation="MTPSGLIVNEVLRLQDPNAKRLGLDRLDEDKLILEFARWAEGQL
                     KKWLDYAKGALLFVMVPEEPESGMFYIYDRARQTFFMLDPAEVDRYGGYRLEEFEQMA
                     QVFGLKALAQNPQVLAETH"
     gene            1541..2521
                     /locus_tag="VFQ79_08490"
     CDS             1541..2521
                     /locus_tag="VFQ79_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139734.1"
                     /translation="MDHRLPATLDMARAFSVLFVNRRAYTIQSTRPHPESGRHYYYRP
                     KAREAGEPVELTLETVRRHLAGEITIGIYAINPITQRCKWMAIDADYKTALEDLIKVQ
                     RQLQDDGIEAALEKSKRGGHLWIFFETPVLAREARIYIYHVAGKLAVQVKGASLADGI
                     EIFPKQDQLRQEEFGNAIRAPLGVHWGARASRGWRYWYYGADYNLDDQLAYLKGLKKV
                     TEQHLQSIIAGKGVPQEFAQRARRTEVPKYFESRRGEFRILDYVQVRRQVGRNWVTRC
                     PSCDAQGHDKSGDNLAISVEEPRKYCCWAGCTKEMIRAAVGRPIEDRRLA"
     gene            2820..3470
                     /locus_tag="VFQ79_08495"
     CDS             2820..3470
                     /locus_tag="VFQ79_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139735.1"
                     /translation="MERRLQEAGIVCQPEVSMQYQTLAKRSVLRGVESGGATKEIGRY
                     FTFCDETGAPIAWLQPIDSVAVNGRHAVVIAPGLVSVDVFRVRNTYDVLIAKHAVVQT
                     AEGQRGRVEARVLFRGRQGHLPLDLAGDDKQMVGRIVPEFFNKAGEPIEIPAEFMSVL
                     KAAVRGANCVGCTHQHYVTTPKAAVPSPILLPIGSIVLPTNEGAGSARSGERRGSQ"
     gene            complement(3788..4198)
                     /locus_tag="VFQ79_08500"
     CDS             complement(3788..4198)
                     /locus_tag="VFQ79_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139736.1"
                     /translation="MRTWVYNPHTGGKSIPFAVKIRTERRILDYAAKHYPGKFTRIDV
                     RFRGALCYIDAYTEPDLPRGSTPPPGETRDQWIDRLRDTPTHLCRIRYFGNEERWSFA
                     WYTYAHEKYEPSFLITGEDHGTPEEAFETSAQFF"
     gene            complement(4211..5668)
                     /locus_tag="VFQ79_08505"
     CDS             complement(4211..5668)
                     /locus_tag="VFQ79_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HEU0139737.1"
                     /translation="MHVDVDDLATYESWDMTAAAIPPRSRFFNLSPAGFGTSMVECLT
                     SYISRLAQAHSVTPGVLHHREVMKYGAPRRNMFSCGIKARSRCFTCIINATGGVATDF
                     VSAIGRLTGRNDLQYLTLIPWKALFPVPMLMRGVAAWCPACLSGWVQAGKPVYVPLLW
                     TLEVVKFCPFHRHPLRVSCPHCGLPQPLLGQCSWVGFCTRCKRWLGSDSGPDGTERFS
                     VLRQETPDWEIWVANQIADMIEAGFHNPPLLTKAQFAQLVRVGTGLEGMSGLARILGV
                     SAGAISHWRMGDRNPILPAYLRLARVLNVTLVDLLTGKVSPDKVESLNLAGMPYWRNL
                     WNRRSALFNRAKAARQLDAALKEVPPPSLQTVQKRNQYHYATLHRHFPEQCRAIQERF
                     REHSAALLRERHANKIAEFRKIAYQLHEQGTTLCVNRILTRMSVPKTLYHPIARRLLA
                     EIKREILTKEHRPAAPRGKMAKQRTSPDALRAQTE"
     gene            complement(5669..6820)
                     /locus_tag="VFQ79_08510"
     CDS             complement(5669..6820)
                     /locus_tag="VFQ79_08510"
                     /inference="COORDINATES: protein motif:HMM:NF017437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0139738.1"
                     /translation="MPEPAFPRELLDQSPEARLGYFKSFTIAHPLLKEADAALKQAIQ
                     EPAGWSLIFVYGPTGVGKTTLRRRVEKHLQESMLETLCQDPGLVPVVSMEAAAPDSSQ
                     FSWKDYYRRALIALDEPLIERKLTHRVRDLFRDASGSVLTNSRAPGLELRMALESALI
                     HRRPAAFFIDEAQHLAKMKSGRKLQDQLDSIKSLASLASTVHVLVGTYELLPFRNLSA
                     QLSRRSIDIHFPRYHAERTEEYTAFRNAVFTLQRHLPLAGESDLLPHLEYCYERSIGC
                     VGLVKDWLTRASALAINQGAHSVSRKMMEKTAWSLDQSERMAREAVDGESEAAENGQK
                     RDRLKQLLQTGARASTPAQARVNPSPPLARTRVGQRKPVRDLVGATMEA"
     gene            complement(6824..9319)
                     /locus_tag="VFQ79_08515"
     CDS             complement(6824..9319)
                     /locus_tag="VFQ79_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEU0139739.1"
                     /translation="MLSPEAMGSWCKEIGVLPHARALIDQIRTSDPARRVGGGRSNVS
                     GRYPSRKMGVTIQFESHRVELAGIYEMEHDLEVLEFYDQPLQIKLNYESPGGKRLGVL
                     HTPDFFVIRRNAAGWEEWKSEEDLLQLAERSPNRYLGSEDGQWRCLPGEACAREFGFY
                     YRVRSSREINWVYQRNIQFLEDYLRSDLPIRAGTHERLRDLVSARFAIGLSDLFRETE
                     GIVTRDEIFAMIALGPLEVDLSAAPLTEPSNVRVLLNHPQAVTCPPAAISASASGPSG
                     AGLLPQMGPTQVAEANRRLKLIRAYQAREPLENTVSARTARHWLLRYRHAEALHGDGY
                     LGLLPRFHLCGNRNRKLPERTRALMSDIIGADYETVKQKRKFHVYGALILACEAEGLA
                     APSYKTFSVAINRRLQGDLVFKRRGRRAAYQLDPPYLELHLTTPRHGDRPFHICHSDH
                     TELDVEVVCSSTGRNLGRPWLTILIDAFSRRLLALFLAFDPPSYRSCMMILRECVHRH
                     GRLPQILVVDGGKEFESVYFETLLARYECTKKTRPPAQPRFGSVCERIFGTSTQLIYS
                     LAGNTQITRNVRQVTTSVDPRNHAVWTLEFLHSRLCEWAYEFYDTTMHPALGQSPREC
                     FTLGLERSGNRPHRQIPYDDEFRMMTLPTTAKGTALVHSGKGVKINHLHYWSTVFRDP
                     AVERTRVPVRYDPYDAGTAYAYVGNRWVSCYSEHFSSFRGRSEREMMIAAAELRKRAQ
                     DHSREFQVTAKRLADFVASVEAEESLLIQRLQDAAARRICQVKCEKDSRLEQDVETSC
                     DCALQSDPDAATLQPSMNAGGFVPPPPEQYEEY"
     gene            complement(9426..9743)
                     /locus_tag="VFQ79_08520"
     CDS             complement(9426..9743)
                     /locus_tag="VFQ79_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139740.1"
                     /translation="MNDETPADVRRWFAAAVGGIWPVATGSISLRKGPCIRKNCPACA
                     AGKGHSSYALYVRRGSRRFSIYVPQRLVPEVRKAIENGRLLQELINEAGLRYVSALKN
                     EEA"
     gene            10133..10333
                     /locus_tag="VFQ79_08525"
     CDS             10133..10333
                     /locus_tag="VFQ79_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139741.1"
                     /translation="MKLPGGQLALPNIDRRLTRADRIALIRRRLNGFELRQAAREQRR
                     RRRPPGAERETRQMNLLESAGE"
     gene            10335..10904
                     /locus_tag="VFQ79_08530"
     CDS             10335..10904
                     /locus_tag="VFQ79_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139742.1"
                     /translation="MSEETVGRHVERRTSEDGKQHWNPCLRDLPSMVLQIFEAGWTEC
                     LYRLDIREFPEWTVAVVRDFEMENFAWSIVPRADKLAAAICGRFDVNPARLTIIEQHD
                     TSWPWWAAAMLGRHTDRRYWVAIAKDGEHRWASEPADRKTVEDLLQRCVEEEGVRIWQ
                     NGEPVALWDWRLRSLCWRFHRPVRTFKER"
     gene            10808..11323
                     /locus_tag="VFQ79_08535"
     CDS             10808..11323
                     /locus_tag="VFQ79_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU0139743.1"
                     /translation="MAKRRAGSVVGLAIALLVLAVPSAGEDFQGKVVRIVDGDTIEVL
                     HSLRPERIRLHGVDAPEKSQAFGSRARQFTAGLVFGRIVTVREKGRDRYGRTLAEIAL
                     LDGRILNRELVANGFAWHYKRYSSDLGLARLEETARAQKRGLWVDAHPVAPWEFRRLE
                     TQKRQGAAIRR"
     gene            11354..11629
                     /locus_tag="VFQ79_08540"
     CDS             11354..11629
                     /locus_tag="VFQ79_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139744.1"
                     /translation="MERVKNASLVVCLFVVLLVIFASYFRATEVGAFFGALAGTPVLV
                     HVFNRLAPPKRERERGRPISAELMPKAEPAPREQVLVLRMEGPNGQR"
     gene            11805..11963
                     /locus_tag="VFQ79_08545"
     CDS             11805..11963
                     /locus_tag="VFQ79_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139745.1"
                     /translation="MHWLPAVAGLGALIAGLIRGITGRGSLRYLVFAGTVSAMKAVTV
                     GIEWLVGQ"
     gene            12053..12724
                     /locus_tag="VFQ79_08550"
     CDS             12053..12724
                     /locus_tag="VFQ79_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139746.1"
                     /translation="MKERSVLYVGLMGLVVGAASWGQQLQTGDEQRTQPSAVWERVNE
                     RKVSLPAGTMLAVRLTDHVSSDKSQAGDRFEGTLDTPVVAGGKVVADKGSSVVGRVVR
                     VRKAGRMTAPSELVLELVELVCLTDRVAIQTDPIERSGETSSHTGDAVAIGRTAALGT
                     ALGAIFGGGKGAAIGAAAGGAVGARGTLGAKGKATAVKSEAVLMFRLRSSATVMVIEK
                     KEARP"
     gene            12994..13593
                     /locus_tag="VFQ79_08555"
     CDS             12994..13593
                     /locus_tag="VFQ79_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139747.1"
                     /translation="MSVGTRSVLFGVHAFWLHPFFTAEAWRRIYGFPWDVRLWVAFFV
                     HDLGYWNSGDIDGPEGEAHVYVGARILRCLFGDAWGEFCLRHSRYWAKKHGCEVSRLA
                     AADKLAFVLTPWWLYLPMARATGELAEYMAVSQERQAGDHSFTESERMLLSSGNSRFW
                     LIGLHAYTRRWVEEHKDRCLRVEEIRAEQALAVSISVRA"
     gene            13604..13855
                     /locus_tag="VFQ79_08560"
     CDS             13604..13855
                     /locus_tag="VFQ79_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139748.1"
                     /translation="MFAQVLSVGRKRWKVLKISFWVLEGVLVLGTAILAWGYSRALLP
                     DLRPCTKTTMGCPAEHRDSRCAGWWCFASGRTSSSCGDR"
     gene            13930..14340
                     /locus_tag="VFQ79_08565"
     CDS             13930..14340
                     /locus_tag="VFQ79_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139749.1"
                     /translation="MGDRVYAEVICRAEDAEVFEDLGFGEQEYWKELPAGVSFLVDEE
                     ANYGHSSSLRELAERGCVFIARHDAGGDYDAARLVSDGTTFSEVDAMVHESRPCVAVN
                     ANGVVDKKQLQAVRKYWRAVARARQKLGFVDEEM"
     gene            14713..14931
                     /locus_tag="VFQ79_08570"
     CDS             14713..14931
                     /locus_tag="VFQ79_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139750.1"
                     /translation="MTTRERLACGGRMLDRIDAQRQRKGDPGIFAAVEGKIVGLELDE
                     LETEWRANSEMGAVLHQPNLSARRSVKP"
     gene            14928..15254
                     /locus_tag="VFQ79_08575"
     CDS             14928..15254
                     /locus_tag="VFQ79_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139751.1"
                     /translation="MIDADLICDFCSAPAPAWRYPARTFLAYCVANVAGESVGDWAAC
                     DACHKLIEAEDRKGLAQRSLDELILKHPETRPAAAALYEDLAGLHDQFFAHRSGTAVP
                     INANAA"
     gene            15276..16781
                     /locus_tag="VFQ79_08580"
     CDS             15276..16781
                     /locus_tag="VFQ79_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0139752.1"
                     /translation="MSAVLRPIEQFVRTLKASRRVSTPLVAIRTADPALTVGRIADAA
                     GKDAAVVQWDSVRGLVKVNEQGGKEMARAVGERNAASVGPVDALVLAGDFGEDAVLVY
                     ANAQRFWNNAEVAQAIWNLRDVFKANGRTLALLTTLGAVLPPELAQDVLVLDEPLPAE
                     EDLSRILEDTIAAAELPALDSDDGSKAVDALLGLAAFPAEQVLAMSLSKKGLDLEQLW
                     ERKRQVIEQVPGLSVWRGGETFDDIGGCENIKRFLTAVIQGEEAPRGVVFIDEIEKAF
                     AGTGTDLSGVKTELTGAILSWMQDRNADGCILIGPPGAAKSLVAKATGNTAGIPTIAF
                     DLGAMQNALVGESGGRLRAALKVVDAVTNGRSLWIATCNSIGTLPPELRRRFTLGTFF
                     FDLPSAEERASIWRIYQQKYGVSGELPDDDGWTGAEIKECCRKAFRLKQSLRESAEYI
                     VPVSRSAAEQVKTLRQQASGKFISASYPGVYQFQEAAAAPRAGRAFRGMES"
     gene            16976..17389
                     /locus_tag="VFQ79_08585"
     CDS             16976..17389
                     /locus_tag="VFQ79_08585"
                     /inference="COORDINATES: protein motif:HMM:NF018565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HEU0139753.1"
                     /translation="MSAYGEFETMVREQRFLIEGLTAMGYRVEEHPEGAALIGYHGDE
                     RPERAEVIIRRAQLDSASNDIGFVREKDGRFRAIVSEYDRSIGYDDKWLGKVHQHYKE
                     KQTLAMAQAKGYVFRGKEVVQTPNGPQIRLQFVAR"
     gene            17400..17600
                     /locus_tag="VFQ79_08590"
     CDS             17400..17600
                     /locus_tag="VFQ79_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139754.1"
                     /translation="MQQKTITVVIDENGNSSVDLEGFAGRGCEKVLKDFQGDDRAKVQ
                     LKKPVFFSQASEAQGRQLKSGR"
     gene            17629..17787
                     /locus_tag="VFQ79_08595"
     CDS             17629..17787
                     /locus_tag="VFQ79_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139755.1"
                     /translation="MVVLTFKTVDDREHEAEFDGREEAIARMDDIPYHVLEYAHVHDE
                     GGDLVAER"
     gene            18039..18641
                     /locus_tag="VFQ79_08600"
     CDS             18039..18641
                     /locus_tag="VFQ79_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HEU0139756.1"
                     /translation="MLLHAFVPATRANGPGLRAVIYFQGCTLNCSGCWNRRTHKFEGQ
                     EVAVRDVAQRFEEARRLEILEGVTFSGGEPMQQAEALRNLMDAIREEAPSVSFGVFTG
                     YTEAELATGQYLTRPKATPERKQDLWRGVQKLLDFAVMGRYDVTQPAMEPLRTSWNQR
                     LVLFSDRYQVADFGPQLIEISVGSTGKTVLTGFPVLGIPS"
     gene            18941..19162
                     /locus_tag="VFQ79_08605"
     CDS             18941..19162
                     /locus_tag="VFQ79_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139757.1"
                     /translation="MAFGYWTKAIFVMVETRLVTVTVLQPYVWASGDRIRRRAAQRVD
                     ATESAELCGPMLVFQDAIPDIRREVSGIE"
     gene            19199..20110
                     /locus_tag="VFQ79_08610"
     CDS             19199..20110
                     /locus_tag="VFQ79_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139758.1"
                     /translation="MPMALIEVRNMGQDLAQKTVCVKVRLGRLGNTRKVSNSQVEVDT
                     DKSLIRVSKHLLDSQELRMIANFDGEVRRYLYGTCLPFDAGIHLCPLGLLEQIEGKLR
                     EFGTRRQELVKAFLAAYPTLCQEAAKRLRSLYNPADYPPLEYVEQQFTFIWQYISFGV
                     PDQLREISTKIWQDEREKAAQVMAEAGLEIQQVLRTAMGELVSHMRDRLKDGPDGKPL
                     RFKETTVSNLVDFLGTFNFRNVTDDAELKSLVDRARTMLAGVSADALRTTGDLRAKVQ
                     QGMADLAAELDTMIVKTPGRKFRFNED"
     gene            20137..20352
                     /locus_tag="VFQ79_08615"
     CDS             20137..20352
                     /locus_tag="VFQ79_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139759.1"
                     /translation="MALDAGFVAGVLEGIEGTLDEVIERLDNSELKASLDALQTTVTA
                     LRVSIEPPTLGPGGNEESEEPVPLEAA"
     gene            20494..20946
                     /gene="ssb"
                     /locus_tag="VFQ79_08620"
     CDS             20494..20946
                     /gene="ssb"
                     /locus_tag="VFQ79_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU0139760.1"
                     /translation="MLGLNNIQIIGNLGNEPETRQLNGNNGQVVNFSVAVNERWKNRE
                     GEPQERTTWLRIVAFNGLGKACADHLIRGSAVYIEGRLQIRTYEDNAGHERTSVEVVA
                     SKIRFLGNGSNNGDEKRPIPGQPKTRKVPASGPPDDFDQAVNSGDCPF"
     gene            21085..>21625
                     /locus_tag="VFQ79_08625"
     CDS             21085..>21625
                     /locus_tag="VFQ79_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139761.1"
                     /translation="MFPTLRRNLLLQLDGKLPNLALMRLSAFLKQRGEAIEFRRIRSP
                     LMLEHGLFDEGWTSVYASLIFERTRPVAERLRQIYPGAIIGGTGWDRRTRLEQIGVTT
                     QALDYSLYPDYPHSIGFSQRGCRLRCPFCVVPDKEGRVTEERPIAEIWRGDPSPRNVL
                     LLDNDFFGAPQWRERVEELR"
BASE COUNT         4498 a         5888 c         7165 g         4074 t
ORIGIN      
        1 gtggaacgat tgcgtgcagc tgcggcgaag gctggcgatc gccggtacgc ggcgttatgc
       61 caggaactga atctggcaag cgattcgatc gacgacattc ccgaccggct gttgcttcgt
      121 cgtctggtgg aaacactgga gagggaaccg aaccgcgcgc aatcgtcgaa cggtggtgac
      181 aacaccgccg ggagtgaagc gcagactgat ggcgcggcgg tcctgagtga tctgcgcggc
      241 cgactgcttc agcaggccag ccgggtgtct ggcgccacaa gacggacact cgcgcaggtg
      301 atcaacgagg cagcgaacgg tgcgttcaag tttgcgaatt tgaaggacct caatccggaa
      361 gatgcgggca aggtgcgcga cgcactggca gcgctcgaac gtatggcgat aacaacgtga
      421 ggcgaccgag ggcggaatga ggaattaagc ggaggcaagc agcttgaagg aaatggtgct
      481 cttcacggac ggatcgtgcc tgggaaatcc tgggcccggt ggatgggcgt gcatcctgcg
      541 atacggaaag cacgagcgcg ttcttcgggg cggggagttg cacacgacga acaacaggat
      601 ggaactttcg gccgcgatct tcgggctgag agcactgatc gagtcatgcc acgtgaccgt
      661 gatcacggac tcccgttatt tgcagcaggg gatgacggga tatctttctc agtggcgaag
      721 ccgggggtgg ctcaacagcc gcggagaaag gatcgccaat cgggatctct gggaggagtt
      781 ggcggcggtg gccagtcggc acgagacaga gtggcagtgg gtacgtagcc atggatcaga
      841 tccagaccaa gatcgatgtg acgcgctggc ccaagagtct gcgaggtcgg tagcggcagg
      901 aaggacgccc ggggaactag cggcctgacc gtatcttcta tatatgggtg cgcactcggt
      961 tgcccagttc caggcttgga gccgctcgag ctccaggctt cctttgtaac ctccacaagc
     1021 acacgacaaa cgaccgagcc aacgtgcgtt cagtcgcagc gcggataccg tgtgcctgta
     1081 agcgattgac acacaaacac aaaaggagaa atgcaatgac cccgagtggt cttatcgtca
     1141 acgaagtgct gcggttgcag gacccgaacg cgaagcgtct ggggctggac cggctggatg
     1201 aagacaaact gatcctggaa tttgcgcggt gggcagaggg gcagctcaag aagtggttgg
     1261 attacgcgaa gggcgccctg ctgttcgtga tggttcctga ggagccggaa tcggggatgt
     1321 tctacatcta cgaccgggct cgacagacgt tcttcatgct ggatccggct gaggtcgacc
     1381 ggtacggcgg atatcggctt gaggagttcg agcagatggc gcaagtcttt ggcttgaagg
     1441 cgcttgcaca gaacccgcag gtgctcgccg agacccactg agatgacttt tgaaaagccg
     1501 agccgtttcg gtgtatagac gagcatagga gaaaacagcc atggaccacc ggctaccagc
     1561 gacactcgac atggcccggg cgttcagcgt gctgtttgtg aaccggcggg cttacaccat
     1621 tcaatccacg cggccgcacc cggagagcgg tcgccactat tactaccggc cgaaagcgag
     1681 ggaggctgga gagccagtgg aactgaccct cgaaaccgtg cggcgccacc tggcgggcga
     1741 gatcacgatc gggatctacg ccatcaaccc gattacgcaa aggtgcaaat ggatggcgat
     1801 cgacgccgac tacaagacgg cgctcgaaga tctgatcaag gtacagcgcc aactgcagga
     1861 tgatggcatt gaggcggcct tggagaaatc caaacgcggg gggcatctct ggatcttctt
     1921 cgagacgccc gtgctggcgc gcgaggcgcg catctacatc taccacgtcg cgggcaagct
     1981 cgcggtgcaa gtcaaagggg ccagcctagc cgacggcatc gagatcttcc caaagcagga
     2041 tcaattgaga caggaagaat tcggcaacgc gatccgggcg cccttgggtg ttcactgggg
     2101 cgctcgggcg agccgcggct ggcgctattg gtactacggc gccgactaca atctggacga
     2161 tcaactggcc tacctgaagg ggctcaagaa ggtgacggaa caacacctcc agagcattat
     2221 cgccgggaag ggggttccac aggagttcgc tcagcgggcg cggcgaaccg aagttccgaa
     2281 gtacttcgag tcccggcggg gcgagtttcg gattctggat tacgtccagg tgcggcggca
     2341 ggtaggccga aactgggtga ctcgttgccc atcgtgcgac gcgcaagggc acgacaagag
     2401 cggagacaat ctggcgatct ccgtcgaaga accgcggaaa tactgctgct gggcggggtg
     2461 cacaaaagag atgatccgcg cagcggttgg ccggccgatc gaggatcgga ggctggcatg
     2521 accggcggag ggtggaggtg gctgcgatcc gtgcgggtat tgatctcgga ctggacggtc
     2581 gtgtcggcat cactattcgt tcttgttggc catgtggtgt ggcgcattga gtcggggaaa
     2641 gtggtctccg gggctattgt gcgcgcagtt ggcggcaatc gtggccgtcg ccgtgggcac
     2701 tgagtactgg gtctcaaaca gaaggagagg ctatgacgac aactacacgg cagcagggaa
     2761 ggaaagaaac ggaaccgcgc cggcaaccca gcttagctgt gagaggagaa cggcgccgaa
     2821 tggagaggcg actacaagaa gccgggatcg tatgccagcc cgaagtatcg atgcaatatc
     2881 aaacgctagc caagcggtcg gtgctgcgcg gcgttgagtc ggggggagcg acgaaggaga
     2941 tcggacgata cttcacgttc tgcgatgaga ccggcgcccc catcgcttgg ctccaaccaa
     3001 tcgactcagt ggcggtgaac gggcgccacg ccgtggtcat tgcgccgggc ttagtgagtg
     3061 tcgatgtgtt ccgggtgcgg aacacctatg acgtgctcat cgcaaaacac gcggtagtgc
     3121 aaacggcgga aggccagcgc ggtcgcgttg aagcgagggt gttgttccgt ggacggcagg
     3181 ggcatctgcc gctcgacttg gcgggagacg acaaacagat ggtgggccgg atcgttcccg
     3241 agttcttcaa caaggcgggg gagcccatcg aaatcccagc ggagtttatg agcgttctca
     3301 aagcggcggt gcgaggggcg aactgtgtgg gctgcaccca tcagcattac gtgacgactc
     3361 cgaaagcagc tgtgccttca cccatacttt tgccgattgg aagcatagta ttgccgacca
     3421 atgaaggggc cggctcggcc aggtcggggg aaaggcgggg aagtcaatga ttctgcttgc
     3481 tctgctcggg ttctcttcgg cgcccccatg tccggagtcg tggcaaaact atgtttccac
     3541 ttcacaacag ttggcaaatc tttgcttcca gttgcggcag gtctgccgga agtgcccaac
     3601 gagaactcag tagtttgcgg gttgcaacgt ggcaaaggta tggttccaat tcggcaaaac
     3661 tatcggtaaa gggacagcag ctgtcacttc acccatagtc ttgccggatt ggaagcatag
     3721 tcttgccact cccaccggat tcatcgctcc cgaagacgcg cgcgtagtgg ccacgcccgg
     3781 aatttcctca gaagaactgg gctgatgttt cgaaagcctc ttcgggtgta ccgtggtctt
     3841 cgccggtgat cagaaagctg ggctcgtact tttcgtgagc ataggtgtac caggcgaagc
     3901 tccatcgctc ttcgttcccg aagtagcgaa tccggcagag atgcgtgggg gtatcccgga
     3961 gccggtcgat ccactgatca cgggtttcgc caggcggtgg agtacttccc cggggcaggt
     4021 ctggttctgt gtacgcgtcg atgtaacaga gagcgccgcg gaagcgcacg tcgattcgcg
     4081 tgaactttcc gggatagtgc ttggcggcgt aatcgaggat ccgccgctct gtacgtatct
     4141 tgacagcgaa cgggatgctc ttcccgccgg tatgggggtt gtaaacccac gtgcgcattc
     4201 tcgatctccc tcactccgtc tgcgcgcgga gggcgtcggg ggaagtacgt tgcttcgcca
     4261 ttttaccgcg gggtgcggct ggtcgatgct ccttcgtcag gatttcgcgc ttgatctcgg
     4321 ccagaagtct gcgcgcgatc gggtggtaga gcgtcttcgg cacggacatc cgcgtgagta
     4381 ttcggttcac gcaaagcgtc gtcccctgct cgtggagctg ataagcaatc tttcggaatt
     4441 ctgcgatctt gttggcgtgt ctttcccgaa gcagggcggc actatgctcc cgaaaccgtt
     4501 cttggatcgc cctacattgt tctggaaagt gtcgatgcag ggttgcgtag tgatattggt
     4561 tccgcttctg aacggtttga agggacgggg gcggcacctc tttcaatgcc gcgtcgagtt
     4621 ggcgtgcggc tttggcccgg ttaaaaagcg cggatcgccg gttccacagg ttgcgccagt
     4681 acggcatgcc cgcgagattg agtgactcaa ccttgtcagg cgacaccttt cccgtgagga
     4741 ggtcgaccaa agtcacgtta agcacacgcg ccagccgcag gtaggccggc agtattggat
     4801 tcctgtcacc catacgccag tggctaatcg caccagcgct gacacccaag attcgggcca
     4861 aaccggacat gccctccaaa ccagttccga ctcgtaccaa ctgcgcaaac tgcgccttcg
     4921 tcagcaacgg cgggttgtga aaccccgcct cgatcatgtc ggcaatctga tttgcgaccc
     4981 agatctccca gtctggtgtt tcctgacgga gaaccgaaaa gcgttcggtc ccatcggggc
     5041 ccgaatcgga gccgagccag cgcttacagc gagtgcagaa gcccacccac gagcactggc
     5101 cgagaagcgg ttgcggaagg ccgcagtgtg ggcaactgac ccgcaggggg tggcggtgga
     5161 acgggcagaa cttgacgact tccaacgtcc agagcaaagg cacatagacc ggcttaccgg
     5221 cctgtaccca accggacaga caagcaggac accaggcggc gacgccgcgc atcaacatcg
     5281 gtactgggaa gagagccttc cagggaatta gggtgaggta ctgaagatca tttcggccgg
     5341 tcagtctgcc gatcgccgag acaaagtcag tcgccacgcc gcctgtcgcg ttgatgatgc
     5401 aagtgaagca acgggacctc gctttgatcc cgcaactgaa catgtttcgg cggggtgcgc
     5461 cgtacttcat tacctcacgg tggtgaagaa ctccgggagt aacggaatgg gcctgcgcaa
     5521 gtcgggagat gtagctcgtc aggcactcaa ccatcgatgt cccgaacccg gccggactga
     5581 ggttgaaaaa ccgactacga ggtgggatgg cggcggccgt catgtcccaa ctttcgtacg
     5641 tggcaagatc gtcaacgtca acgtgcattc aagcctccat ggttgctcca acaagatccc
     5701 gaactggttt gcgctggcca acacgcgtac gcgcgagcgg aggtgacgga ttcacacggg
     5761 cctgcgccgg tgtcgaagcc ctagcccctg tctgcaggag ctgtttcaag cgatctcgct
     5821 tctggccatt ttcagcggcc tcgctttcac cgtcgaccgc ctccctggcc attctctcgg
     5881 actggtccag cgaccacgcc gtcttttcca tcatcttgcg gctcactgag tgggcgcctt
     5941 ggttaatggc cagcgccgag gctcttgtca accagtcttt aaccagcccg acacagccga
     6001 tggaacgctc atagcagtac tccaggtgag gcagaaggtc ggactcgccg gccaggggca
     6061 gatgtcgttg aagcgtgaag acggcgttcc gaaacgccgt gtactcctct gttctctcgg
     6121 catgataccg gggaaaatga atgtcgatgc tacgccggct taactgcgca ctgaggttgc
     6181 ggaaggggag caactcgtat gtccccacca atacgtgaac cgtactggcc aggctggcca
     6241 gagatttaat cgagtcgagc tggtcctgca gtttgcggcc gctcttcatt tttgccagat
     6301 gctgggcctc gtcgatgaag aacgccgccg gccgtcggtg gatcagcgcg ctctccaggg
     6361 ccatccgcaa ctcaagccct ggcgcacgag agttcgtgag cacgctgccg ctggcgtctc
     6421 gaaacaagtc gcggacccga tgcgtcagct tccgttcgat gagcggctcg tccaaggcga
     6481 tcagagcccg ccggtagtaa tctttccagc tgaactggct ggaatcgggc gccgccgctt
     6541 ccatgctgac caccgggacg agcccggggt cctgacaaag cgtctccaac atggattcct
     6601 gcaagtgctt ctccactcgc cggcgcaacg tcgtcttgcc cacgccagtg gggccgtaga
     6661 caaaaatgag ggaccaacct gccggttcct gaatggcttg ctttaaggcg gcatcagctt
     6721 ccttgagaag aggatgcgca atcgtgaacg acttgaaata gccgagccgt gcctcaggtg
     6781 actggtctag cagttctctc gggaatgcgg gttcgggcac gattcagtac tcctcatatt
     6841 gctctggtgg cggcggcacg aagccgccgg cattcattga tggttggaga gtggctgcat
     6901 cagggtcgga ttgcagtgcg cagtcgcatg aggtctcaac atcttgctcc agccggctat
     6961 ctttttcgca tttgacctgg cagattctcc tggctgctgc atcctgaaga cgctggatga
     7021 ggagcgattc ttctgcttcg acggaggcga cgaagtcggc gagcctcttg gcggtcactt
     7081 ggaattcacg ggagtggtct tgcgctcgtt tacgcagctc ggcggcggcg atcatcatct
     7141 cccgctccga ccggccacga aacgacgaga agtgttcgga gtagcagctg acccagcgat
     7201 ttccaacata ggcgtaggcg gttccggcat catacgggtc gtatcggacc ggcaccctgg
     7261 tgcgctcaac ggccgggtct cggaaaacgg tggaccagta gtgcagatgg ttgatcttga
     7321 cgcctttgcc ggagtggacc aaggcggtgc ccttcgccgt ggtcggcaga gtcatcattc
     7381 gaaactcgtc atcgtagggg atttggcggt gagggcggtt cccagaacgc tccaatccca
     7441 aagtgaaaca ctcccgggga ctttgcccga gagctggatg catcgtcgtg tcatagaatt
     7501 cgtaggccca ctcgcacaga cggctgtgga ggaactccag ggtccagacg gcgtgattcc
     7561 tcggatcaac actggtcgtc acctggcgca cgttcctggt gatctgagta ttgcccgcca
     7621 aactgtagat gagttgggtg ctggtcccaa agatccgttc gcagacggat ccaaaccgtg
     7681 gctgggcggg aggccgggtc ttcttcgtgc attcgtagcg tgcaagcagc gtctcgaagt
     7741 acacgctttc gaactccttt ccgccatcga ccacgaggat ttgcggaagg cgcccgtgcc
     7801 ggtgaacgca ctctcgcaga atcatcatgc atgagcggta cgatggcggg tcgaaggcca
     7861 gaaagagggc caacaaacgc cgagaaaatg cgtcaatcag aattgtcagc cagggtcggc
     7921 cgaggttccg ccccgtgctg gagcaaacga cctccacgtc gagttcggtg tgatcggagt
     7981 ggcagatgtg aaaaggccgg tcaccgtggc gcggcgtggt gaggtgtagt tccaggtaag
     8041 gcggatcgag ctgataggct gcccgtcggc cgcgtcgttt gaaaaccaaa tccccttgca
     8101 gacgccggtt gatcgccacg gaaaaggttt tgtagctcgg cgccgcaagt ccttcggctt
     8161 cgcaggcgag gatgagcgcg ccgtacacgt ggaacttccg cttctgcttg acggtctcgt
     8221 agtctgctcc aatgatgtcg gacatgagcg cccgggttct ttccggaagc ttgcggttcc
     8281 gattcccgca aagatggaag cgcgggagca gaccgagata gccgtccccg tggagcgcct
     8341 ctgcatgccg gtatcgcaat agccagtgtc gggcggtcct ggctgaaacg gtgttttcca
     8401 acggctcccg tgcctgatag gcccggatca gcttcaaccg ccgattcgcc tccgcaacct
     8461 gtgtaggacc catttgtggc aggagccccg cgccggacgg accagaggca ctggcgctta
     8521 tcgcagcagg gggacacgtc acggcttgag ggtggttgag aaggacacga acattgctcg
     8581 gttcggtcag cggcgcggcg gagaggtcga cttcgagagg tccaagggcg atcattgcga
     8641 agatctcgtc gcgtgtcacg atgccctccg tctctcgaaa gagatcgctc aatccgattg
     8701 cgaagcgggc ggagacaagg tcgcggaggc gttcatgcgt tccggcacgg attggcagat
     8761 ccgatcgaag ataatcctcc aggaactgga tgttccgctg gtacacccag ttgatctcgc
     8821 gggaagaacg gacccggtaa tagaacccaa attccctggc gcatgcttcg cccggcagac
     8881 agcgccactg gccgtcctcg gagccgagat aacggttcgg actgcgctcc gcaagttgca
     8941 ggagatcctc ttcggatttc cactcctccc aaccagcggc attccggcgt atgacgaaaa
     9001 agtcaggtgt gtggagtacg cccaggcgct ttccgccagg gctttcgtag ttcaacttga
     9061 tctggagggg ctgatcgtag aactcgagga cctcgagatc gtgctccatc tcatagatgc
     9121 cggcgagttc cacacgatgg ctctcgaatt gaatggtgac gcccatcttc cggctgggat
     9181 agcgtccgct gacgttcgac cggccgccac ccacgcggcg agccgggtca gatgtgcgga
     9241 tttgatcgat gagcgctcgg gcatgcggca ggacgccgat ttccttgcac caactcccca
     9301 tcgcttcggg actcagcatt ggcagcctcc tcaaaatacc ggcgctctcc gcagtacact
     9361 gaccacctcg ccggtgtttc gtcccgtgat cgacacagga caggattccc atattcctgg
     9421 ggacgctacg cctcctcgtt tttgagcgcg ctcacgtacc gcaaaccagc ttcgttgatc
     9481 aactcctgca acaatcggcc attttcaata gccttacgaa cttcggggac caacctttgc
     9541 ggaacatata tcgaaaagcg gcgcgatccc cggcgtacgt agagtgcgta gctgctgtgg
     9601 cccttgcccg cggcacacgc cgggcaattc ttgcgaatac agggaccttt cctcaacgag
     9661 atggatcccg tcgccacagg ccagatcccg ccgacggctg ctgcgaacca gcgccggaca
     9721 tctgcaggag tctcatcgtt catctgtata tgtgccagta tgcatgtata cagaacagaa
     9781 gtcaagcatt ttgtttcggt cctggctacg ctccggagac ttttgaatta atgcgtccgg
     9841 ccagactata cttccacatg ccaattacgg cgaaactatc cttccagggc cggtcctcag
     9901 cgggtaccgg cgatggcgaa tctgaggttt ctggccatgc aacggcaaag gtatggttcc
     9961 agtagcggca aaactatggt tcaaggcaca gcagcagctg ctgctcctgc tgcggccgcg
    10021 agggcggcaa ccaatggtgt cactgcgccc gcggctgaat cggcacctga tcccgcgctg
    10081 gcggtccgag ccaacggaag ccctgagagc gtgtaacgca gaggatcaaa ccgtgaaact
    10141 ccctggtggc caacttgcgc tccccaacat cgaccgacgt ctgacgcgcg ccgaccggat
    10201 cgcgctgatt cggcgccggc tcaacggctt tgagctgcgc caggccgcac gcgaacagcg
    10261 ccggcgccga agacctccag gggcagaacg cgagacgcgg cagatgaatt tattggagag
    10321 cgccggagag tgacatgagc gaagagacag tggggcggca cgtcgagcgc cgcacgtcag
    10381 aagacggaaa gcagcactgg aacccatgcc tgagggatct gccgtccatg gtcctgcaga
    10441 tcttcgaagc cggttggacg gaatgtctat acaggctcga cattcgggag ttcccggaat
    10501 ggacagtagc cgtcgttcgg gacttcgaga tggagaactt cgcttggagt atcgtcccgc
    10561 gagccgacaa gctggccgcg gcgatctgcg ggcggtttga tgtgaatcca gcgcggctga
    10621 ccatcatcga gcaacacgat acgagctggc catggtgggc ggcggcgatg ctcggcagac
    10681 acacggaccg gcggtattgg gtggcgatcg ccaaagacgg cgaacaccgc tgggcttccg
    10741 aaccagcgga caggaagacc gtggaggacc tgctccagag gtgcgtggag gaagagggag
    10801 ttcggatatg gcaaaacgga gagccggtag cgttgtggga ctggcgattg cgctccttgt
    10861 gctggcggtt ccatcggccg gtgaggactt tcaaggaaag gtagtcagaa tcgtggacgg
    10921 cgacacaatt gaggtccttc acagcctacg accagagcgg attcggctcc acggcgtgga
    10981 cgcgcccgag aagtcacagg ctttcgggtc gcgggcgcgc caattcaccg ctgggctggt
    11041 gttcggccgc atcgtgacgg tccgagagaa ggggcgagac cgctacggcc ggacgctggc
    11101 ggagatcgca ctgctggatg gccgcattct caaccgggaa ctcgtagcga acggctttgc
    11161 atggcactac aagcgctact ccagcgacct cggtctggcg cgattggaag agacggcgcg
    11221 tgcgcagaag agggggttgt gggtcgacgc ccacccggtt gctccgtggg agttccgaag
    11281 gcttgagacg cagaaaagac agggtgccgc gatccgccgg taggtggaga gacggttcta
    11341 agaaggagac ccgatggaac gagtgaagaa tgcaagctta gtcgtctgcc ttttcgttgt
    11401 actgctggtg atcttcgcca gctacttccg ggccacagaa gtcggcgctt tcttcggagc
    11461 actcgcggga acgccggtcc tggtccatgt cttcaatcgt ctggcgccgc cgaaacggga
    11521 gcgagagcga gggcggccta tctcggcaga attgatgcca aaggcggaac ccgcgccgcg
    11581 agagcaagtc ctggtgctgc gcatggaggg tccgaatggg cagcggtgac gcgggtgaac
    11641 cggctccggg gcggtcgctg tcggtggtga tctggctcat cgtgagtggg ggcgtgtttt
    11701 ggtgcttgtg gacaagctgg caagaagggg tgccgtggga tcggatcgtc gaaccgcgtc
    11761 gggagccggg gttcattctt gtgacaacgc cggatccgat agtcatgcac tggttgccag
    11821 cggtcgcagg gcttggagcg ctgatcgccg ggctcatccg tggcatcacg ggacgtggaa
    11881 gcctgcgtta cttggtattc gcaggaacgg tgtcggcgat gaaggccgtg acggtcggga
    11941 tcgagtggct tgtgggccag tgaactctcg gagaagtcaa tacgactcgc cgcaaatatg
    12001 gccggtgact gatagccgaa cagagagtcg cggaaatcga aggatggttg aaatgaaaga
    12061 acgatcggta ttgtacgtgg gactgatggg cttggtcgtg ggcgcagcaa gttggggaca
    12121 gcagttacag acgggcgacg agcagcgaac gcaaccgtcc gcggtatggg aaagggtcaa
    12181 cgaacggaag gtctcgctgc cggccggaac catgctggcc gtacggctca cagatcatgt
    12241 ctcgagtgat aagagccagg cgggtgaccg cttcgaggga acgctcgata cgcccgtcgt
    12301 ggcaggcgga aaggtcgtag cggacaaggg cagttctgtc gtcgggcgcg tggtgcgagt
    12361 acgaaaggcg gggcgaatga ccgctccctc cgagctggtg ctcgaactgg tggaactggt
    12421 ttgcctgacg gatcgcgtgg caatccagac tgatccgatc gagcggagcg gcgagacttc
    12481 gtctcacacc ggggacgcag tggcaatcgg caggacggca gcgttgggca cagcgctggg
    12541 agcgatcttc ggcggtggca agggcgccgc gatcggcgct gccgccggag gcgccgtggg
    12601 tgccagagga acactcggtg cgaaggggaa agcaacggcg gtgaaaagcg aagccgtact
    12661 gatgttccgt ctgcgatcgt cggccacggt catggtgatc gagaagaaag aagcgcggcc
    12721 gtgaaagttg cagcagaaca ggcgcggctg cccaagttgc agacccggcc tccgattagc
    12781 ctgccgtcgg tcttctgtct cgaccgccgg acgcaactgc atagatagtc gatggcaccc
    12841 ctaaaacgtt gtcgccgccg aaacgatgct gtcgaaggca gtcggaggat cattcggtgg
    12901 cattcggcgc tgagacaccg gatcggcggg gcatggatag atgtcggtca attccgcgcg
    12961 agcgtttact ttgtcgctta gaggagggta cttgtgagcg tcggcactcg aagcgttttg
    13021 tttggggtac acgccttctg gttgcacccg ttcttcactg cggaggcttg gcgacggatc
    13081 tatggttttc cctgggacgt tcgcctctgg gtggcattct tcgtccacga tctgggctac
    13141 tggaacagcg gggatatcga tgggccggag ggcgaggcgc atgtctatgt cggagcccgt
    13201 atcctgagat gcctctttgg agacgcatgg ggcgagtttt gtttgcgtca ctcacgctac
    13261 tgggccaaaa agcacggctg tgaggtctcg cgtttggcgg cggctgacaa gttggcgttc
    13321 gtgctgactc catggtggct ctatttgccg atggcgcgcg ctaccggcga actggccgag
    13381 tacatggcgg tgtctcagga gcgccaagcc ggcgatcatt catttacgga atccgagcgc
    13441 atgctgctat cgagcggcaa ctcacgcttt tggttgatcg ggttgcacgc atacacgagg
    13501 cgctgggtgg aagagcataa ggaccgctgc ctacgggtgg aggaaatccg cgccgagcag
    13561 gcgcttgcag tctccatttc agtgcgtgcc tgagggatga accatgtttg cgcaagtcct
    13621 gtcggtcgga cggaagagat ggaaggtgct gaagatcagc ttctgggtgt tagaaggcgt
    13681 tctcgttctg ggcacggcga ttctggcgtg gggctatagc cgcgcgctgt tgccggacct
    13741 gcggccctgc acgaagacga caatgggctg cccggctgag catcgtgata gtcgctgcgc
    13801 tggatggtgg tgctttgcat cggggcgaac gtcatcatcg tgcggcgatc gttagcacca
    13861 cagattaatc acaaaaaaac ggccacggcg gcggtctgct cgtggccgtt tcgcttttta
    13921 agaggagcca tgggagatcg ggtctacgcg gaagtcattt gccgcgctga agacgcggaa
    13981 gtgttcgagg acctcgggtt cggagaacag gagtactgga aggaactgcc ggcgggagtc
    14041 tcgttcctgg tggacgagga agccaactac ggccattcct cttctctgcg ggagttggct
    14101 gagagagggt gcgtcttcat cgctcggcat gatgcgggag gagactacga cgcggccagg
    14161 ctcgtgagcg acgggacaac cttttccgag gtcgatgcga tggtccacga atcacggccg
    14221 tgcgtggcag taaacgccaa tggcgtggtc gacaagaagc aacttcaggc ggtgagaaag
    14281 tattggcgcg cggtcgcgcg agcgaggcag aaactaggct tcgtagacga ggagatgtaa
    14341 gtgacgccgc cgaacgcgaa tcaaagccag tgaggcacgg cggcatagcg tagcggcatg
    14401 gggaccaagc ggccgacgcc ggactcaaga ccggcgtcgg ccgctttgtg cttttcagga
    14461 gggccagatg cggaggctgc aaacgaaaca gaccgaggct tggcgatgtc gccgggaaca
    14521 gtaccttcgg gaatcagggc gtcacgttga cttgatcaag gaaggttacg cgtctccaga
    14581 ccccacgacg agctggatcg tgaccgttgg aggacgcgcg ggggcttcga tggagcgcaa
    14641 tccagggatg aaactgttcc tggtgaaacg acagacgagc gtaatgaaca tgccggaggc
    14701 ggaggtgggc ttatgacgac gagagaacgt ctggcgtgcg gtgggcgcat gctggaccgc
    14761 atcgacgcac agcggcagag gaagggcgat ccagggatct tcgccgcggt agagggcaag
    14821 atcgttggtc tcgaactgga cgaactggaa acagagtgga gagcgaattc ggagatgggg
    14881 gccgtattac accagccgaa cctctccgcc aggaggagtg taaagccatg atcgacgcag
    14941 atctgatttg cgatttctgc tcagcgcccg ccccggcgtg gcgataccct gcgcggactt
    15001 tcctcgctta ctgcgtggca aatgtcgccg gtgagagtgt tggcgattgg gccgcatgcg
    15061 atgcgtgtca caagctgatc gaggcggaag accggaaagg tcttgcgcaa cgctcgctgg
    15121 acgaactgat cttgaaacac ccggagacaa ggccggccgc tgccgcgctg tacgaggatc
    15181 tggccggact tcacgaccag ttctttgcgc accgcagcgg cacggcggtg ccaattaacg
    15241 ccaacgcggc gtgagcgcga ggaaggagaa aggcaatgtc ggcggtgctt cgacccatag
    15301 aacagtttgt gcgaacgttg aaggcctcac gccgggtttc cacgccgctc gtggcaatcc
    15361 ggaccgcaga cccggcgctg acggtaggcc gtattgcgga tgcagcagga aaagacgcag
    15421 cagtcgtgca gtgggacagc gtgcgcggcc tcgtcaaagt gaatgaacag ggcggaaagg
    15481 agatggccag ggcggtcggc gaacggaatg cggccagcgt tgggccggtc gatgcccttg
    15541 tgctcgccgg tgatttcggc gaggatgctg tgctggttta cgcgaacgct caacggttct
    15601 ggaacaacgc cgaggtggcg caggcgatct ggaatctgag agacgtgttc aaagcaaacg
    15661 gccgaacgct ggctcttctg acaacgctag gtgcggtctt gccgcccgag ctagctcaag
    15721 acgtgctggt gctcgacgaa cctctgccgg ccgaggagga tttgagcaga attctagagg
    15781 acaccatcgc ggcggcggag ttgccggcgc tggactcgga cgacggcagc aaggccgttg
    15841 acgccctgct tggactggcg gcgtttcccg cggaacaggt cctcgcgatg tcgctgtcga
    15901 agaagggtct cgatctggaa cagctgtggg agagaaagcg gcaggtgatc gagcaggtgc
    15961 cggggctgag tgtgtggcgg ggtggagaga ctttcgacga catcggagga tgcgagaaca
    16021 tcaaacgatt cctgacggca gtcatccagg gagaagaagc gccgcgtggg gtggtgttca
    16081 tcgacgaaat cgagaaggct ttcgcgggaa caggtacgga cctgtcgggc gtgaagacag
    16141 agctcactgg cgcgatcttg tcgtggatgc aggaccggaa tgcagacggg tgcatcctga
    16201 tcggcccgcc cggagcggcc aaaagtctcg tcgcgaaggc taccgggaac acggcgggga
    16261 ttccgacaat cgctttcgac ctgggggcga tgcagaacgc gctggtaggc gaatccgggg
    16321 ggcgtctgcg agctgcgctc aaggtagtcg atgcggtcac aaacgggcga agcctgtgga
    16381 ttgcgacgtg caattcgatt ggcaccctgc cgccggagct gcggcggcgc ttcacgctcg
    16441 ggacgttctt ctttgatttg ccgtcggctg aggagcgcgc ttcaatctgg cggatctacc
    16501 aacagaaata tggcgtttcc ggcgaattgc cggacgacga cggatggacc ggagcggaga
    16561 tcaaagagtg ttgccgaaag gcgttccggc tcaagcaatc gctgagggag tcggcggaat
    16621 acatcgttcc ggtgtcgcgc tcggctgcgg aacaggtcaa gaccttgcgg caacaagctt
    16681 caggaaaatt catcagcgcg tcgtaccctg gcgtgtatca gttccaggag gccgcggcgg
    16741 cgccgcgagc gggacgtgcg tttcgcggca tggagagcta acggggccgc gtgcgggcgt
    16801 tccctcggat atcacagcgt atggagggaa ccgagacgct tacgggcgac ctgcaagctc
    16861 gtgaacccgt ttttgttgtc ttcgttagcg gtcacgtggt ggttttcaaa tcgaccggtt
    16921 gacggcacaa gtgaccgtcg tcgtcgtgta ggagatcgag aaaggagcac aggaagtgtc
    16981 ggcatacgga gagttcgaga ccatggttcg ggaacagagg ttcctcatag aagggctgac
    17041 cgcgatggga taccgagtcg aggagcatcc cgaaggtgcg gccctgattg gctaccacgg
    17101 tgacgagcgg ccggagcgcg cggaagtcat catcaggcgg gcgcaactgg acagtgcctc
    17161 gaacgacatc ggcttcgtgc gcgagaaaga cggccgcttc cgagcgatcg tgagcgagta
    17221 cgaccgcagc atcgggtacg acgacaagtg gctgggcaag gtgcaccagc actacaagga
    17281 aaagcagact ctcgcgatgg cgcaagcaaa ggggtacgtc tttcgcggca aggaagttgt
    17341 tcagacaccg aacgggccgc agattcgcct gcagtttgtc gcgcgatagg agcaaaggca
    17401 tgcagcagaa gacgatcacg gtggtcattg acgagaatgg caacagttcg gtggacctcg
    17461 agggctttgc aggccgcggc tgcgagaagg tgctgaagga ctttcagggg gacgaccgcg
    17521 ccaaagttca gcttaagaag ccggtgttct tcagccaggc aagcgaggcg caaggtcggc
    17581 agttgaaatc gggcaggtaa ggaccgccgg tctgacgagg agtctgcgat ggtcgttctc
    17641 actttcaaga ctgtggacga tcgcgaacac gaggcggaat tcgatggccg tgaggaagcg
    17701 atcgcgcgaa tggacgacat cccctatcac gtgctcgaat acgcgcatgt gcatgacgaa
    17761 ggcggcgatc tagtcgcgga gcgctgatgg tgcaaagagg ttgacggcag gcaatcaaga
    17821 gctgtcacgt acacgcaccc gtttggtttc gtttctccgt gacgagccaa aaggtgaacg
    17881 ttacgcggtt ttgacggaag gcggcgagag tttattcggg aatcgcgagc ccagtttcgc
    17941 agagtccgtg gagatgcgta ggcatctggc ggcgacatcg gagatgcggc gagcggtgta
    18001 gccggagtag agccgagccg ccaacgtgag ggggcggtat gcttctacat gctttcgtgc
    18061 cggcaacccg ggcaaacgga ccgggactgc gcgcggtgat ctacttccag gggtgcacct
    18121 taaattgcag cggctgttgg aatcgaagaa cgcacaagtt cgaaggccaa gaggtggccg
    18181 tgcgagatgt agcgcaacgg tttgaagaag cacgccggct ggagatactt gaaggcgtta
    18241 cattctcggg cggcgagccg atgcaacagg ctgaggcgtt gcgcaacctg atggatgcga
    18301 tccgcgaaga ggcgccttcc gtgagcttcg gcgttttcac cggatacacg gaagccgaac
    18361 tggcaactgg acagtacctg actcgcccga aggcgacccc ggagcgcaag caggatttgt
    18421 ggcgcggggt tcagaaactg ctcgactttg ccgttatggg ccgttacgac gtaacacagc
    18481 cagcgatgga gccgctacga acaagctgga atcaaaggct agtcttgttc agcgaccgct
    18541 atcaggtggc cgacttcggc ccgcaactta tcgagatcag cgttggcagt acggggaaga
    18601 ctgtgctcac gggatttccg gtactcggca ttcccagctg aggcaaccgg ccacggatag
    18661 gcgtcaacgc acggcgcttc cggcaatcgc accacaacaa cggaagtgtg aagagtcaca
    18721 ggtcggcgtt tgtgtccgaa tgcaaagagg ctcaagcgct gcggaactgc gcccttgaac
    18781 ttgtcgaaat cttgcgaagc gacctcggtg gaactaaagt cacgacaccc gtcgtgcacc
    18841 tggtgcaaag ggactgcctt gaaaacgagc gttcgtcccg ggaggggatg gcatcgaagg
    18901 agtcgaccgg tccctgctgt cacacctcgc gctggaacca gtggcgttcg gttactggac
    18961 caaagcgatc ttcgtgatgg ttgagacacg gctggtcacg gtaacggttc ttcaaccata
    19021 cgtctgggcg agcggggatc gaattcggcg ccgcgccgcg cagcgcgtcg acgccacgga
    19081 gagcgccgaa ttgtgcgggc cgatgctcgt ttttcaggat gcgattcccg acatcaggcg
    19141 tgaagtctcc gggatcgagt gatcaacaac aggaaaggag aagcaggaaa tggcagcgat
    19201 gccgatggct ttaattgaag ttcgcaatat gggccaggat ctggcccaga agaccgtttg
    19261 tgtcaaggtc cggctcggcc ggctcgggaa cacgcggaag gtcagcaatt cgcaggtcga
    19321 ggtagacacg gacaaaagtc tgatccgcgt ctcgaagcat ctcctggatt cccaggaact
    19381 gcgcatgatc gccaatttcg acggcgaggt ccggcgctac ctctacggca cctgcctgcc
    19441 atttgatgca ggaattcatc tttgcccgct cggactgttg gagcagatcg agggaaaact
    19501 gcgggagttc ggaacacgca gacaagagtt ggtgaaggcg ttcctggcag cgtaccccac
    19561 gctgtgtcag gaggccgcga aaaggctgcg atccctatac aacccggcgg attacccgcc
    19621 gctcgagtac gtggaacagc agttcacctt catctggcaa tacatcagct tcggtgttcc
    19681 cgaccaactg cgcgaaatct cgacaaagat ctggcaggac gaacgcgaga aagcggcgca
    19741 ggtgatggcg gaggcgggcc tcgagatcca gcaggtgctg cgaacggcga tgggcgagtt
    19801 ggtgagccat atgcgggatc ggctcaagga tggaccggac ggcaagccct tgcggttcaa
    19861 ggaaactacg gtgtcgaacc tggtggactt tcttggcacg ttcaatttcc gaaacgtcac
    19921 cgacgatgca gagctgaaat ctctggtgga cagggcgcga acgatgctgg caggcgtgag
    19981 cgccgatgca ctccgcacga ccggtgatct gcgcgcgaag gttcagcaag gcatggccga
    20041 cctggcggcc gaactcgaca cgatgattgt gaaaacaccg ggccgcaagt tccgcttcaa
    20101 cgaggactga ttggcctgga gaaaggagga accccgatgg cgctcgacgc aggatttgtc
    20161 gctggtgtct tggagggtat tgagggcacg ctcgacgaag tgatcgagcg cctcgacaat
    20221 tccgaactca aggccagcct ggatgccctt cagacgaccg tgacggcgct tcgggtttcg
    20281 atcgagccac cgacccttgg gccgggaggc aacgaggaat ccgaagagcc ggttcccctc
    20341 gaagcggcgt aagccgaccc catccgttaa tgcccgaggg cgttctgccc gtggggtcaa
    20401 tctcaacaat ctagccgcac tgaaagcccg tagggatctg tgtgtccgag cgggcagtgc
    20461 gttccgtacg aacaactgaa aggaaggaac gacatgctgg gtttgaacaa catccagatc
    20521 ataggaaatc tgggcaacga gccagagact cgccaattga acgggaacaa cggccaggtg
    20581 gtcaatttct cggtggccgt caacgagcgg tggaagaacc gcgaaggcga gccccaggag
    20641 cggaccacct ggctccgaat cgtcgcgttc aacgggctcg ggaaggcctg cgcggatcac
    20701 ttgatcagag gttccgccgt ctatatcgaa ggccgtctcc agatccgtac ctacgaagac
    20761 aatgccggtc atgagcgcac ttcggtcgaa gtggttgcgt ccaagatccg attcctcggc
    20821 aacgggagca ataacggcga cgagaagcga ccgataccgg ggcaacccaa gaccaggaag
    20881 gtgccggctt cgggtccgcc ggacgatttc gaccaagccg tgaattcggg cgactgtccg
    20941 ttctagggta tttgggggag tgtccggttt ctggcgcgcc actccctcag tgcctcacag
    21001 gagcgtggac gagattcaca ccccgcggat gttgcgcgcc tcgcccacga acagtccata
    21061 ccgcactagt ccggaggaat tccgatgttc ccgactctca ggcgcaatct gttgctccag
    21121 ctcgatggca agttgcccaa tctcgcactg atgcgtttaa gcgcgttctt gaaacagcga
    21181 ggtgaggcga tcgagttccg gcgcatccgt tctccgctga tgcttgagca tggcttgttt
    21241 gacgagggct ggacgtctgt gtacgcgagc ctcatattcg aacgcacgcg gccggttgcg
    21301 gagcgccttc gacagatcta tccaggcgcg atcataggcg gaaccggttg ggaccggcgc
    21361 actcggctgg agcagatcgg agttaccacg caggcgctcg actactcgct ctatcccgat
    21421 tacccgcact cgatcgggtt cagtcagcgg ggatgccggt tgcggtgtcc gttttgcgtg
    21481 gttcccgaca aggaggggcg cgtcacggaa gagcgcccta ttgcagagat ctggagggga
    21541 gatccctccc cacgcaatgt actgctgctc gataacgact tcttcggcgc gccgcagtgg
    21601 cgcgagcgtg tggaggaact gcggg
//
LOCUS       DASTMK010000197         8100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_393337, whole genome shotgun sequence.
ACCESSION   DASTMK010000197 DASTMK010000000
VERSION     DASTMK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393337"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="VFQ79_08630"
     CDS             <1..1836
                     /locus_tag="VFQ79_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEU0139762.1"
                     /translation="LQPVVLPGRDWLHEKPGDRDKVMWLHVFGRLQPGVRIETAQAAA
                     NVTFQQGLTAFYGSVERARRQWLKLRPAASGASQIRGQFAAPLTMLLVASGLVLLIAC
                     ANLGNLMLARTTGRIREMSVRLALGAGRGRLIRQLLTESMVVAFLGGMAGLGAAWILR
                     AGLLRLVPETIPLPASPDARILGFAFVLTLVVGLILGLLPALRTMSVNASVGLKAQGR
                     GLTGSAAWLRMGKLIVVGQVALSLPLLVGAGLLLRTLQNLQQVDLGYVKERLIQVRVD
                     VQMGGYEEQRRLPLFQRLLERVRAVPGVRAASYSKSGLFLGSRSSGDVEVEGYMQKGD
                     GGVWSVWDHVGPDYFSTLGIPLLQGREITERDQPSRNKVCVINEVFARQFFAGRNPLG
                     MHVNRYEIVGVARDSRNRNLRDEIEPRFYVPAGQPADVPPRFITFAVRTVAEPSSVLA
                     GVRQAILAEDPNLPVTAASTLTELIDRQMVQDRMLARLSMAFGVVALLLAALGLYGVL
                     SYGVARRTSEIGIRKALGAQHGAVMRMILRETGLLLVLGLIAGVALSAAGIRFITSRL
                     YGLAPTDPLALGAAIAVLTGVALLAAWLPAYRASRVDPLVALRYE"
     gene            complement(1842..2948)
                     /locus_tag="VFQ79_08635"
     CDS             complement(1842..2948)
                     /locus_tag="VFQ79_08635"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEU0139763.1"
                     /translation="MNRRQVLSVSAGAALAGAVPSPALTMQAGSPKRAQMKLGCQTGP
                     TDDRRLQFFKRHSVEHICGYPEDPENKGYYSVEDLSRLRDRCEKNGVNLDMIAPPFLA
                     STHIDRTPRPAIMLGQSPDRDRDIEHVQNMIRNCAKAGVPAIKYNMSILGVLRTERTS
                     GRGGSTYSTWRLKEAKASGKFAPETRAGRVQADLYWERITYFLDRVIPVANEYKIRMA
                     CHPHDPGVPPEGFQGVDAVLGTVGGLKRFISIRESPYHGLNFCQGTVSEMLQDPGKEI
                     YDVIRYFGSRKKIFNVHFRNIRGRRDDFQEVYPDEGDIDFVRAMTVYRDVGYPYMMMP
                     DHVPHHPDDKGGIQAFAFAYGYIRALIQAVDRIA"
     gene            2934..4529
                     /locus_tag="VFQ79_08640"
     CDS             2934..4529
                     /locus_tag="VFQ79_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0139764.1"
                     /translation="MSAIQRRYSLKDLSGTLSRQAGGAETARFGCVRIVKQYFRYRMN
                     RRTFLVSSLAPVHASALQAHPPAPFGAVPSPRQMRWHALEQYAFLHFTVNTFTDKEWG
                     YGDEDPNLFHPTAFDAGAIANALAAGGMKGVILTCKHHDGFCLWPTKTTQHSVRSTKW
                     RGGKGDVVKEISAAAREHGLQFGVYLSPWDRNTPLYGGPKYVEMYRVQLRELLTQYGP
                     IFEVWHDGANGGDGFYGGAREKRTIDKRTYYDWPGTWDLIRKLQPDAVIFSDIGPDVR
                     WVGNEKGIAAETCWATISPVGADGGPPAVGDVRQSDNQTGHRNGRQWLPAECDVSIRP
                     GWFWHERENDKVKTPVQLFDLYSKSVGRNASFLLNVPPDRRGLLHENDVKSLAGFGAT
                     LRDTFARNLAAGAKLQSSSVRGAAYRPENLLDGSRDTCWAARDGVLEPEVIFELRRPE
                     TFDVIRVREAIQFGHRVDSFAVDIFRDNAWVRCAESTSIGACRLILLPEPVTAGRLRL
                     RITKAAASPALAGFGLYRTSQQG"
     gene            4676..5944
                     /locus_tag="VFQ79_08645"
     CDS             4676..5944
                     /locus_tag="VFQ79_08645"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU0139765.1"
                     /translation="MNSQNGQNGKSNPYALAMQNFEAAADLLELNADIRNMIRAPERA
                     LSVTVPVRMDDGHIQCFEGYRVQHNTSRGPAKGGIRFHPNVTLDEVKALATWMTWKCA
                     VVDIPFGGGKGGVTCNPKFLSMAEIERLTRRYTTAILPIIGPGQDIPAPDVYTNSQIM
                     AWIMDTYSMTRGYLIPGVVTGKPISLGGSLGRNEATGRGVFHAIESACQHLRLPVKGA
                     KIVVDGFGNAGSVAAHLLDGAQADVIAVSDSRGAVYNPEGLHIPKLMLHKEKTGTVCN
                     FPGGECIAPVELLKLECDILIPAALENTVHGGNAPHVRAKVIAEAANGPLTPDADAIL
                     REKDTFVIPDILCNAGGVTVSYFEWVQNEQHLRWDLNEVNTRLEDVMKRSFNEVLEIQ
                     LAFKSSMRTAANVLAIRRVVEATRLRGHYP"
     gene            6007..8088
                     /locus_tag="VFQ79_08650"
     CDS             6007..8088
                     /locus_tag="VFQ79_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0139766.1"
                     /translation="MTLRRKPLLKWLLVALAAVGAASAQSRVYRVVDVQSVRAAGDPA
                     QWVRTVQVPEKVAEVRCDILVAGGGMGGVAAAMRALDRGHTVCLTEATDWLGGQMTSG
                     GVSALDEHKFIELTGGTRTYYELRRRVRQHYREQFTLAPQLAGRIDLNPGACYVSSLC
                     FEPRVGVQVIEDMLGPRKTKLQRFMRTEIIDLEVSGGMIQSALAYRLDRGEVFRIRPR
                     FVLDATELGDLLPLAGVPYTVGSEPRSETGEPHAGEKANPACVQSFTYPFAIERTATP
                     AHGSAKPPEYESFRDSQRFSFLLTYPPELGWKGPVQYVMFGEDPPIPNNQSPASFFSW
                     RRLLARKNFTGESAPADLALINWPRQDYSAESLLDREPLDFARVLQQAKRASLAFLYW
                     LHTEVPRDDRQGSGYPELKLRPDVMDTDDGLSKYPYIRESRRLKTGATVIEQDIVAEY
                     QPGSRARWFADSVGTAFYMVDIHPCGVGERGRMMMPRPFQIPMGTMIPQRVRNLLAAG
                     KNIGVTHVTNGAFRLHPVEWNVGEAAAVIASLTLARGSLPQPRPVQEELAAAGVPLVW
                     FDDLPVDHPAFAAIQLTAMSRIYPLSNTDLHAAPDAPVTRVEAAQALFVLFAAKPGSP
                     PPPADAAISVAVEHGWMATDHRNWFHPDLPFLWTDWREEKLPFTLPPMDTKRNGPVTR
                     AELAQRLVKAR"
BASE COUNT         1506 a         2428 c         2540 g         1626 t
ORIGIN      
        1 ctgcagcctg tggtgctgcc gggccgcgac tggctgcacg agaaacctgg cgaccgggac
       61 aaggtgatgt ggctccatgt tttcggccgc ctgcagcccg gcgtacgcat cgaaacagcc
      121 caagccgccg ccaacgtcac atttcaacaa gggctaaccg ccttctacgg ctcggttgaa
      181 agagccagga gacagtggct caaactgcgg ccggctgcca gcggcgcgtc acaaatccgc
      241 gggcagtttg cggcgccctt gacgatgctc ctggttgctt ccgggcttgt actgctgatc
      301 gcctgcgcga atctgggcaa cctgatgctg gcccggacta ccggaaggat acgtgagatg
      361 tccgtacggc tcgcgctggg agcaggccgg gggaggctca tccggcagtt gcttaccgaa
      421 agcatggtgg tcgcgtttct aggtggcatg gccggtttgg gcgctgcctg gatccttcgc
      481 gccggattac ttcgtctggt cccggagacc attcctctac cggcctctcc ggacgctcgc
      541 atcctgggct tcgccttcgt tctgacgctc gtcgtgggac tgattcttgg ccttcttccg
      601 gcgctgcgaa cgatgagtgt gaacgccagc gtgggattga aggcgcaggg acgcgggctt
      661 acggggtcgg ctgcatggct gcgtatgggt aagctcatcg tcgtcgggca agtggcgctg
      721 tctcttccat tgctggtcgg agcgggcctt cttctgcgga cactgcaaaa cctgcaacag
      781 gtggacttgg gttatgtgaa ggagcgattg atccaggtga gggtggacgt gcagatgggc
      841 ggatatgaag agcaacgccg cttgccgtta ttccagcggc tgctggaacg ggtgcgcgca
      901 gtacccggcg tgcgcgcagc gagctattca aagagtggtc ttttcctggg ctcgcgaagc
      961 tcgggcgatg tcgaggtcga gggttatatg cagaagggtg acggcggggt ctggtcggtt
     1021 tgggaccatg tcgggcccga ctatttctcg acactcggca tcccgttact gcagggccgc
     1081 gaaatcaccg aacgggatca gccgtcgagg aacaaagttt gtgtgatcaa cgaggtgttc
     1141 gcccggcagt tcttcgccgg ccgcaaccct ctcgggatgc acgtcaacag gtacgagatc
     1201 gtgggtgtgg cgcgggactc ccgcaaccgg aatctgcgcg acgaaatcga gcccaggttt
     1261 tatgtgcctg cggggcagcc ggccgatgtg ccgccgcgct tcatcacatt tgcggttcgg
     1321 actgtcgccg agccctccag cgtgctcgcg ggtgtgcggc aggcgattct cgcggaggat
     1381 ccgaacttac ccgttacggc cgccagcaca cttacagaac tcatcgatag gcaaatggtg
     1441 caggaccgga tgttggcgcg tctctcgatg gcattcggag ttgtcgcgct gctgttggcc
     1501 gcgctcggcc tttatggagt gctttcgtat ggcgtagcgc ggcgcacgag cgagatcggc
     1561 atccggaaag cgctaggcgc ccaacatggc gctgtcatgc ggatgattct acgagaaacc
     1621 ggattgctgc tggtcctggg gctgattgca ggcgtcgcct tgtcggcggc cggaatacgg
     1681 ttcatcacga gcagactcta cggtctcgcc ccaacggatc cgttggcgct tggagcggca
     1741 attgcagtgc ttacaggtgt ggcgttgtta gcggcctggc ttcccgcgta ccgtgcatcg
     1801 cgagtcgatc cgctggtcgc gctacggtac gagtagatcg ctcacgcgat tcgatccacc
     1861 gcctggatca gcgcccgtat atacccgtag gcgaacgcaa atgcctggat ccctcccttg
     1921 tcatcgggat ggtgcggaac gtggtccggc atcatcatgt agggatagcc cacgtcgcgg
     1981 tatacggtca tcgctctcac gaaatcgata tcgccctcgt cggggtagac ttcctgaaaa
     2041 tcgtcacgcc tcccgcggat attgcggaaa tgtacattga agatcttttt gcgcgagccg
     2101 aagtagcgga tcacatcgta aatctctttg cccggatcct gtaacatttc ggagacggtt
     2161 ccctggcaga agttcagacc gtgataggga ctctcgcgaa tcgaaataaa tctcttcaac
     2221 ccgcctaccg tcccgagcac ggcgtctact ccctgaaagc cttccggcgg cacacccgga
     2281 tcgtgcggat ggcacgccat cctgatcttg tattcgttgg cgaccggtat tacacggtcc
     2341 agaaagtagg taatccgctc ccagtacagg tctgcctgca cgcgccccgc gcgcgtttcc
     2401 ggcgcgaact tgccggacgc cttcgcttcc ttgaggcgcc atgtgctgta agtggacccg
     2461 ccgcggcccg atgttcgttc ggtgcgcaga acccccagaa tgctcatgtt gtacttgatg
     2521 gcgggaactc cggctttggc acagttccgg atcatgttct ggacgtgctc gatgtcccgg
     2581 tcccggtccg ggctttggcc gagcatgatg gcgggtctgg gagtgcggtc gatgtgggtc
     2641 gatgccagaa acggaggcgc gatcatgtcc aggttgacgc cgttcttttc gcagcggtcc
     2701 cgcaagcggg acaggtcttc cacggaatag tagcctttgt tttcggggtc ttccggatag
     2761 ccgcagatgt gctccacgga atggcgtttg aaaaactgga gcctgcggtc gtccgtaggt
     2821 ccagtctggc atcccagttt catctgtgcg cgcttcggcg agcccgcctg catggtaagc
     2881 gcgggggacg gaacggctcc cgccagcgcg gccccggccg atacggaaag aacttgtctg
     2941 cgattcaacg gcgctactcc ttaaaagatc tttccggcac tctatcacgc caggcaggcg
     3001 gcgcggaaac tgctcgcttt ggctgcgtcc gcattgtgaa acaatatttc cgttacagga
     3061 tgaaccgccg gacatttctc gtttcctcgc tggcgcccgt ccacgcatcg gcgcttcaag
     3121 cgcacccccc cgcgcctttc ggagcggtgc cgtcgccgcg gcaaatgcgc tggcacgcgc
     3181 tcgagcagta tgcgtttctg catttcaccg tgaacacgtt cacggacaag gaatggggtt
     3241 acggcgacga agatccgaac ctgtttcacc ctacggcgtt cgacgccggt gccattgcca
     3301 atgcgctggc ggcgggcggc atgaaaggcg ttattctcac ctgcaagcat cacgacggct
     3361 tttgcctctg gccgacgaag accacgcaac actccgtccg cagcacaaag tggcgcggtg
     3421 gaaaaggcga tgtcgtaaag gagatctccg cagccgcgcg agagcatgga cttcaattcg
     3481 gcgtctacct gtcgccctgg gatcgcaaca cgccgctcta cggcggcccg aagtacgtcg
     3541 aaatgtaccg cgtgcaattg cgcgagttgc tgacacaata cggtccgatt ttcgaagtct
     3601 ggcacgacgg cgccaacgga ggcgacggct tctacggcgg cgctcgcgag aagcgcacca
     3661 tcgataagcg aacctactac gattggcccg ggacctggga tttgatccgg aaactccagc
     3721 ccgatgcggt catcttcagc gacatcgggc cggacgttcg ctgggtcgga aacgaaaaag
     3781 ggatcgctgc cgaaacctgt tgggctacta tttcgccggt tggcgcggac ggtggacccc
     3841 cggctgtcgg cgacgtccgg caaagcgata atcagaccgg gcatcgcaac ggcagacagt
     3901 ggctgcccgc ggaatgcgat gtgtccatcc gtccgggatg gttctggcat gagcgcgaaa
     3961 acgacaaggt caaaacccct gttcagctct tcgatcttta ctcgaagtcc gtcggccgca
     4021 acgcgagttt tctgttgaat gtgccgccgg accggcgtgg attgctgcat gagaacgacg
     4081 tgaaatcgct ggccggtttt ggtgcaacgc tgcgcgatac gttcgcgcgc aatctcgccg
     4141 ccggggcgaa gctgcaatct tcgagtgtgc gcggcgccgc ataccggccg gaaaatctgc
     4201 tggacggcag ccgtgacacc tgctgggcgg cgcgcgatgg cgtactggag ccggaggtga
     4261 tcttcgagct tcgccgccct gaaacattcg acgtcattcg cgtgcgtgag gcaatccaat
     4321 ttggacatcg tgtggactcg ttcgccgtcg atatattcag agacaacgca tgggtgcgtt
     4381 gtgcggaaag caccagcatc ggcgcttgcc gcctgatcct tctgcccgaa ccggtgaccg
     4441 ccggtcgcct acggctgcga atcaccaagg ctgccgcatc tccggcgctg gccgggttcg
     4501 ggttatatcg aacttcgcag caaggctgaa cccttcggga cgattcgcgc tgatgtatcc
     4561 tgtaggagaa tcgcaatagt tcatggaatc gttgcatccc gcctggtgat catccgctcg
     4621 ggctgagatg gattcgcctc acagtttgtg aaaacgctac aggagtaaga cagtcttgaa
     4681 ttctcaaaac ggccagaacg gcaagagtaa tccctacgct ctcgcaatgc agaatttcga
     4741 agcggccgcc gaccttctcg aactgaatgc cgacatccgg aatatgattc gcgctcccga
     4801 acgcgccctc tccgtcacgg ttcccgtgcg aatggacgac ggacatattc agtgcttcga
     4861 gggatatcgc gttcagcata atacttcgcg cggccccgcg aaaggcggta tacgctttca
     4921 tcccaacgtc acgctcgatg aagtaaaagc tctcgccacc tggatgacct ggaagtgcgc
     4981 ggtggtggac atcccgttcg gcggtggaaa gggcggcgtt acgtgcaatc cgaagttctt
     5041 gtccatggcc gaaatcgaac gcctgacgcg gcgctacacg acagccatcc tgcccatcat
     5101 tggacccggc caggacattc ccgctccgga cgtctacacc aattcccaaa tcatggcctg
     5161 gatcatggac acctacagca tgaccagggg ctacctgatt cccggcgtcg tcaccggcaa
     5221 gcccataagc ctcggcggtt ccctgggacg caatgaagcc accggccgcg gagtgttcca
     5281 cgccatcgag agcgcctgtc agcatctccg gcttcccgtc aagggagcca agattgtggt
     5341 cgacggcttt ggaaacgcgg ggtccgtggc cgcgcatctg ctggacggcg cgcaagcgga
     5401 tgtcatcgcg gtaagcgact cccgcggcgc tgtttataac cccgaaggat tgcacattcc
     5461 gaagctgatg ctgcacaaag agaagaccgg caccgtgtgc aattttccgg gcggcgagtg
     5521 catcgctccc gtggaactcc tgaagctgga atgcgacatc ctgataccgg cggctcttga
     5581 aaacaccgtt cacggcggca acgcgcctca cgtgcgggcg aaagtgattg cggaggccgc
     5641 caacggcccc ctcacacccg acgcggatgc cattttgcgc gaaaaggaca ccttcgtcat
     5701 cccggacatt ctctgcaatg ccggcggcgt tacggtctcc tacttcgaat gggtgcagaa
     5761 cgaacagcac cttcgctggg acttgaatga agtgaatacc cggctcgagg atgtcatgaa
     5821 gcgcagcttc aacgaagtgc tggagattca actcgccttc aaatccagca tgcgcaccgc
     5881 ggcgaacgtc ctcgccatcc ggcgtgttgt cgaagcaacc aggttgcggg gccattatcc
     5941 ataagaccgg cgcggccggc tctgttccgc tactctggac ctccggccac catcggcagc
     6001 gttgagatga cactgagacg aaagcctctc ctgaaatggc tgctggttgc gctcgccgcc
     6061 gttggggcag cgagcgcgca gagccgcgta taccgtgtag tcgatgtgca gtctgtacgc
     6121 gccgcgggcg acccggctca atgggtgcgg accgtgcagg tgccggaaaa ggttgcggaa
     6181 gtacgctgcg acattctggt agcgggtgga ggcatgggtg gcgtcgcggc tgcgatgcgt
     6241 gctttggatc gcggtcatac ggtgtgtctg accgaggcga cggattggtt aggcggccag
     6301 atgacctccg gcggcgtctc ggctctcgac gagcacaaat ttatcgagct cacgggtggc
     6361 acgcgtactt actacgagtt gcgtcggcga gttcgccagc attaccggga acaattcaca
     6421 cttgcgccgc aactggccgg ccggatcgat ttgaacccgg gcgcctgtta tgtttcctcc
     6481 ctctgcttcg aaccgcgcgt gggggtacag gtgatcgagg acatgctcgg cccgcggaaa
     6541 acaaagctcc agcgctttat gcgcaccgag atcatcgacc tcgaagtaag cggcggcatg
     6601 attcaaagcg cgttggcgta tcgcctggat cgtggcgagg tcttccgcat ccggccgagg
     6661 ttcgtattag atgccaccga attgggggac ctgctgccac tcgccggcgt tccctacacc
     6721 gtcggctccg agccgcgcag cgagacgggt gagccgcacg caggcgagaa ggcgaacccc
     6781 gcgtgcgtgc agagcttcac ttatccgttt gcgatcgagc gcaccgcgac acctgcgcac
     6841 ggcagcgcaa agccgcctga atacgaatct tttcgggact cgcagcgctt cagttttctg
     6901 ctgacgtatc cgcccgagct cggttggaaa gggcccgtgc agtacgtgat gttcggagaa
     6961 gatccaccca ttccgaataa ccagtctccc gcgtcattct ttagttggcg gcggctgttg
     7021 gcccggaaaa atttcaccgg cgagagtgcg ccggcggacc tcgcgctgat taactggccg
     7081 cggcaggatt attcggcgga gtcgctgctc gatcgcgagc cgctcgattt cgcgcgcgtg
     7141 ttgcagcagg caaagcgtgc atcgctcgcg ttcctttact ggctgcacac ggaggtgccg
     7201 cgagacgacc ggcagggcag tggctatccc gagttgaaat tgcgtcccga tgtgatggac
     7261 acggatgacg ggctgtccaa gtatccgtac atccgggaat cacggcgtct gaaaacaggg
     7321 gccacggtga tcgaacagga tatcgtcgcg gaatatcagc ctggaagccg ggcgcgctgg
     7381 ttcgcggatt ccgtgggcac ggccttctat atggtcgaca ttcacccctg tggcgtggga
     7441 gagcgcgggc gcatgatgat gccccggccg tttcagattc ccatgggaac gatgattccg
     7501 cagcgggttc gcaacctgct ggcggcgggc aagaacatcg gcgtcaccca tgtgacgaac
     7561 ggagccttcc gcctgcatcc ggtggaatgg aacgtgggtg aggcggccgc cgtgatcgcc
     7621 agcctcacgc tggcccgggg ctctttgcca cagccgcggc cggtccagga ggaactggca
     7681 gcggcgggag tgcccctggt gtggttcgac gatctgccgg tggaccatcc cgccttcgcc
     7741 gcgattcagc ttactgcgat gtcgcggatc tatccgcttt cgaataccga tctgcacgcc
     7801 gcgccggatg ccccggtaac tcgcgttgag gccgctcaag cgctgttcgt gctattcgcg
     7861 gcgaagcccg gctctccgcc gccgcccgcg gacgcggcga ttagtgtggc cgtcgagcac
     7921 ggctggatgg caacggacca ccgcaactgg ttccatccgg acctgccctt tctctggacc
     7981 gactggaggg aagagaaact gcctttcacc ctgcctccga tggatacgaa acgcaacggc
     8041 ccggtcaccc gcgccgaact ggcacaacgg ctggtcaagg ctcggtaacg cgtgtcagct
//
LOCUS       DASTMK010000198         7974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_425609, whole genome shotgun sequence.
ACCESSION   DASTMK010000198 DASTMK010000000
VERSION     DASTMK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425609"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2835
                     /locus_tag="VFQ79_08655"
     CDS             <1..2835
                     /locus_tag="VFQ79_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139767.1"
                     /translation="GRNFTQLGLLQAGVVPLTNGLISAGGSIKATQAFAVNGQRPESN
                     NYLLDGVRNVNRMDGGFAIRTPIDALQEFRILTHTAPPEYGSASGAAVSVVTRSGGNS
                     MHGGLYYFGRNDVVDARNFFSADVEPLKQHQFGATLGGPLRRNKLFFFGYYEGFRNRQ
                     GVTKTTTVPTPAERAGDFSALQDPSTNNLLINFLTGQPFPGNRIPSGMFNPVSQRVLE
                     FYPLGNVSPSLFRATEVLRNDVDQGGVRIDAIASERDQFAVRYATSAGSNFNPLSIRG
                     ADVPGFPAGDDITTHSATVSHTHVFGPNALNTLRAAFFRNRFLFDQRFNHTTPRSLGF
                     QYDNTFEPALGPPYFIVNGYASVGNPITGPRNSVQNDYEIYEAFSLVRGAHTIKIGGD
                     FRRTHINISQGIAANGFFVFAPFPTNSSFANFLIGFPVVFFQGGGDFQRGLRSFEVSG
                     YAQDQWRVTRKLTLNYGVRYEIHSPFAEIRDRLNQFAPGRQSQVFPDAPRGLLFPGDP
                     GVAKRILNIYYKGLMPRVGLAYDPTGSGRTSLRAAYGIFYDAFSNGSSMPMQAAISAL
                     PWLQAVQLGPPALNYTDPWGASGVPFQPGFFPKPVTMLTENPVGRPPYAQNWNFSIQQ
                     SLPSAVLFEIRYVGTKGTRVPRFIEANPSVYTPGASSQSVDARRIHAGCPAEGGPCDF
                     ASVGLVDYSTNSTYHALQTTLSRRFHRGLTFSTSYWFSKTLDYVSSMNVAGSAPRLVS
                     GENDIAQNPFDLRAEHGPSLFDAKHRWTASATWELPFGKSLRGISRALAAGWQLNGIA
                     TVSSGTPFTVYDTRNVAQQGSHPEITGFAGSRPDLIAKPNGGPRTVEQWISPSAFRRL
                     DAVTEAGRFGNAGRNIARGPGQGTLDLSAFKNWSLSEHLRVQLRIEAFNVTNHANFGV
                     PVNDLVSPNFGRILEAGSPRVFQAGLKILF"
     gene            2844..3110
                     /locus_tag="VFQ79_08660"
     CDS             2844..3110
                     /locus_tag="VFQ79_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HEU0139768.1"
                     /translation="MKDEPMEPMFLPDVENNPRPGPYYDNLRRMQEAGLEYPQIWHLF
                     AFLPQATGHLARFTQEIMRGPAPISPGLRELIAAYTSYRNDCPF"
     gene            3162..3455
                     /locus_tag="VFQ79_08665"
     CDS             3162..3455
                     /locus_tag="VFQ79_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139769.1"
                     /translation="MWGVLRDLESSSLEEKEKALFRFIDKVNHDSPRITADDMKPLYA
                     AGWNDEAIYFAITVCSLFNFYNRWIDATGVHALSDEAHREGGKRMAMHGYDRG"
     gene            3442..4701
                     /locus_tag="VFQ79_08670"
     CDS             3442..4701
                     /locus_tag="VFQ79_08670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0139770.1"
                     /translation="MTGGSGQAAVIDRPRIYPGWLVVAAAFCGVMVSFGSLLVFTFGV
                     FLKPLSSEFGWSREGISTAFGIAAMTVAVCSPMLGRLLDRYGARRVVLFCMAVFGLAV
                     GSLSLLTPNLVHLYAAFFVLGAVGNGTTQMGYSRAVASWFDDRRGFALAVVVAGVGVG
                     SMLFPWLAQRIIDAHGWRTAYLVLGAMVLVLGIPLTALFVREHPGSVGPQNTAAGSSV
                     RDGLHSRAFWLLVAALFLSSIAINGAITHLAPLLTDRGISAEHSALAVSVLGAASLTG
                     RLVTGNLLDRCFGPAVSFALLAGVAAGIFLLATASSSGTGLLAAALIGFGLGGEADVT
                     PYLLTRYFGLRMFSTLYGLTWTAYAIAGAIGPIVMGRVFDVTQSYSALLTVLSALTLG
                     AAMLMLFLPAYPLKEQRDRVAGGLKCA"
     gene            4692..5003
                     /locus_tag="VFQ79_08675"
     CDS             4692..5003
                     /locus_tag="VFQ79_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139771.1"
                     /translation="MRLKLLGMILGGLAFLYFAVAACLYVAMRQPPERFGAIMSKVPM
                     AAMMVLPFKSLWMSARAGPLAVGDTAPDFALPVLDRSRTVRLSEEWQTRPVVLIFGSY
                     T"
     gene            5016..5402
                     /locus_tag="VFQ79_08680"
                     /pseudo
     CDS             5016..5402
                     /locus_tag="VFQ79_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
     gene            5449..7545
                     /locus_tag="VFQ79_08685"
     CDS             5449..7545
                     /locus_tag="VFQ79_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0139772.1"
                     /translation="MRRLRLLSAFAITVTVALAAGQAGAGETYDVVVVSGSSGGFGAA
                     LAAGRMGMRVALIEDTPVLGGMLSNGISNIDTYSFESLSGIFEEFRSRVKEHYRPLMA
                     TDPIFRAQRLRDYKPRQSNVAAEGGRWEPHVADRILKEMIAPVPGVTIFYDTWATDVV
                     LQGKHATGVVTMNKRGEKRVFTGKVIIDATHEADVAAWAGVPYRVGREARSPLEPHAG
                     DIYFFNGTGEIMEGSTGRQDRAIVSYGLRLCIQRYGGEETSHILASPPPGYDRSKYTH
                     SPYSGEITMPKSKAEMNANPVGNEMQEVNWVWPEASRAERLELYQQYKNHALGFLYFL
                     QHERGLKHLGLPKDEFVDNGHVPYRIFVREARRVEGEATMTEADINPFLTGRGLIPAL
                     RKDSIAIGHYPIDSKPVRGKTDMSTPEKGAGDFFLANVVTPFQVPYGAIVPAQVEGLL
                     VPVAISATHVAFSAIRMDPTWMVMGQAAGVAAALSIRENVMPRQLPIERLQRELIRQK
                     VRLLFYWDVPLDHPAFGAIQWLSVSKGLQGYPDRLFRPDQLLSRAEMSVLIANVFDLW
                     PSVSNFHFTDVPWSHWAFREIETLFNNRLLTPFGVRPRWPDAGGYDPVRNAGFRQGQT
                     FSAFEPDRPVTWGEMVGVLRTLQSRQIHPPVEWVNGIVKDSEFAVSLQKKAILPAEAL
                     TRAEACMLVAAVSERR"
     gene            complement(7546..>7974)
                     /locus_tag="VFQ79_08690"
     CDS             complement(7546..>7974)
                     /locus_tag="VFQ79_08690"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEU0139773.1"
                     /translation="LIFQNTGEVFNEMLAAQMVPVITHPERNSLLYSRTADLAAWAAR
                     GVCLQVTAQSLLGRFGRDARDFAHGLIERGLVAFLASDAHDCIDRPPRLDLGYRHLAE
                     RYAERVARALCIENPRAVLTGDPLPMPPEPVKSNRRWWFW"
BASE COUNT         1481 a         2440 c         2403 g         1650 t
ORIGIN      
        1 gggcgcaact ttactcaact tggattgctg caagccggag ttgtgccttt gacaaacggc
       61 cttatcagcg ccggcggctc catcaaagcg acgcaggcat tcgcggtgaa cgggcagcgt
      121 cccgagtcga acaactatct gctggacggg gtgcgcaacg tgaaccggat ggacggcgga
      181 ttcgcgatcc ggacgccgat cgatgcgctt caggaattcc gcatcctcac gcataccgcg
      241 ccgcccgagt acggcagcgc aagtggggcc gcggtctctg tcgtcacgcg gtccggcgga
      301 aactccatgc atgggggcct ctactatttc ggacgcaacg acgtggtgga cgcgcgcaac
      361 ttcttttcgg cggatgtcga accgttgaag cagcatcaat tcggcgccac cctcggcggt
      421 cccctgcgcc ggaacaagct gttctttttc ggttactacg aaggattccg gaatcgccag
      481 ggtgtgacga agacaacgac ggtgccgacg ccggccgaac gggcgggcga cttctcggcg
      541 cttcaggacc cgagcacaaa caatctgctg atcaattttc tgacgggaca gccgttcccg
      601 ggtaatcgta ttccgagcgg gatgttcaac ccggtgtcgc agcgagtgct cgaattctat
      661 ccgctcggca acgtctctcc ctcactgttc cgcgccaccg aagtcctgcg gaacgacgtc
      721 gaccaaggcg gcgtccggat cgatgcgatt gcgagcgagc gggaccagtt cgccgtgcgt
      781 tatgcaacgt ccgccggatc gaacttcaac ccgctttcca tccgcggcgc cgacgtgccg
      841 ggatttccgg ccggcgatga catcacgacc cattccgcga ctgtgtcgca cacgcacgtg
      901 ttcggcccga atgccctgaa caccctgaga gcggctttct tccgcaaccg gtttctgttt
      961 gatcaacgct tcaaccacac cacgccgcgc tccctgggct ttcagtacga caacaccttc
     1021 gagccggccc tcgggccgcc ttacttcatc gtgaacggct atgcgagcgt tggaaacccg
     1081 attaccggac cgcgcaacag cgtgcagaac gactacgaga tctacgaggc gttttcgctg
     1141 gtgcggggcg cgcacaccat caagatcggc ggcgacttcc ggcgcacaca catcaacatc
     1201 agccagggca ttgcggcaaa cgggttcttc gtgttcgcgc catttcccac caacagctcc
     1261 tttgcgaatt tcctcatcgg ctttccagta gtgttcttcc agggcggcgg cgactttcaa
     1321 cgcggtttgc gcagctttga ggtaagcggc tacgcgcagg accagtggcg cgtcacacgc
     1381 aaactcacac tgaactacgg agtgcgctac gagatccatt cgccatttgc cgaaatccgc
     1441 gaccggttga atcagtttgc acccggccgg cagtcgcaag tctttccgga cgcgccgcga
     1501 ggacttctgt ttccgggcga ccccggagtg gccaagcgca tcctcaacat ctactacaaa
     1561 ggattgatgc cgcgtgtggg attggcctat gaccccacag gaagcggccg gacgagtttg
     1621 cgcgcggcgt atgggatttt ctacgacgct ttttcgaatg gctcgagcat gccgatgcag
     1681 gcggccatca gcgccctccc gtggttacag gcggtgcaac tcggtccgcc ggcgttaaat
     1741 tacaccgacc cgtggggagc aagcggcgta ccgttccagc ccggcttctt tccgaagccg
     1801 gttaccatgc tgaccgaaaa tcccgtggga cgtccgccgt atgcacagaa ctggaacttc
     1861 tcgatccaac aatccctgcc ctcggcggtg ctctttgaga ttcgctatgt aggcacgaag
     1921 ggaacgcgtg ttccacgctt catcgaggcc aatccatcgg tctacactcc gggcgcgtct
     1981 tctcaaagtg tggacgcccg gagaattcac gccggctgtc ccgcggaagg gggcccgtgc
     2041 gatttcgcat cggtcggcct ggtggactat tccaccaact ccacttacca cgcgctgcaa
     2101 acaacgttgt cgcggcgctt ccatcgcggt ctgacgttca gcacctccta ttggttctcc
     2161 aaaactctcg attacgtttc gtcgatgaac gtcgccggat cggcgccgcg ccttgtttcg
     2221 ggtgagaacg atatcgcgca gaatccgttc gatctgcgcg ccgaacacgg gccctcgctg
     2281 ttcgatgcca agcatcgctg gaccgcgagc gctacctggg aattgccttt cggcaaatca
     2341 ttgcggggca tttcacgagc actggccgcg ggctggcagc tcaatgggat tgcgacggtc
     2401 tcctccggta cgccgttcac ggtttacgac acacgcaatg tggcccagca gggctcccat
     2461 ccggagatta ccgggttcgc gggcagccgg ccggatttaa tcgccaaacc gaatggcggt
     2521 ccacggacgg tggagcagtg gatttcgcct tccgcgttcc ggcgccttga cgctgtcact
     2581 gaggcaggac gtttcggcaa cgcgggccgc aacatcgcgc gaggtccggg tcagggaacg
     2641 ctggacctct ccgcatttaa gaactggagt ctctcggagc acctgcgcgt acagttgcgc
     2701 atcgaagcct tcaacgtgac caatcatgcc aatttcggtg tcccggtgaa cgacctggtg
     2761 tctccgaatt tcgggcgaat cctggaagcc gggtccccgc gggtcttcca ggcaggactc
     2821 aaaatcttgt tctagggcgt gccttgaaag acgaacctat ggagccgatg ttcctccccg
     2881 atgtggaaaa caacccgcgg ccgggtccct actacgataa tttgcgccgg atgcaggaag
     2941 ccgggctcga atacccgcaa atctggcatc tgtttgcttt tcttccgcaa gcgaccgggc
     3001 acctggctcg tttcacgcag gagatcatgc gcggcccggc tcccatcagt cccggtcttc
     3061 gcgaattgat cgcggcctat acgtcttatc gcaacgattg cccgttttga ctgaaggccc
     3121 acgccgcggt cgcggcggaa ctgctgggaa gcgagcaact ggtgtggggc gttctccggg
     3181 atctggagag ctcgtctctt gaggaaaagg aaaaggcact gttccgcttt atcgacaaag
     3241 tgaaccacga ctcgccgagg attacagcgg acgatatgaa gccactctac gcagccgggt
     3301 ggaatgacga ggcgatctac ttcgccatca cggtttgttc gttattcaac ttctacaatc
     3361 gttggatcga cgcaaccgga gttcacgcgt tgtccgatga ggcgcaccgc gaggggggca
     3421 agcgcatggc gatgcatgga tatgaccggg ggtagcggcc aggcggcagt catcgaccgt
     3481 ccccgtattt atcccgggtg gctggttgtc gcagccgcgt tctgcggtgt catggtgagc
     3541 ttcggctcgc ttcttgtctt cacgtttggc gtgttcctca agccgctcag cagcgaattc
     3601 ggatggagca gagaaggtat ttccacggct ttcggcattg ccgccatgac ggtcgccgta
     3661 tgctctccaa tgctgggccg cctgctcgac cgctacggcg cgcgccgtgt agttcttttc
     3721 tgcatggccg tgttcggact ggctgtgggc tcgctttcct tgctgacgcc aaacctggtc
     3781 cacctgtatg cggccttctt tgttcttgga gccgtgggga atgggaccac ccagatgggt
     3841 tattcccgcg cggtagcgag ttggttcgac gaccggcgcg gcttcgcgct ggcggtggta
     3901 gtggccggcg tgggagtcgg gtcgatgctc ttcccatggc tggcccaacg gatcatcgac
     3961 gcgcacggat ggcggaccgc ctatctggtg cttggggcga tggtcctggt tctgggaata
     4021 cctttgaccg ccctcttcgt tcgggagcat cctggcagcg ttggtccaca aaacacggct
     4081 gccggcagct cggttcgcga cggactccat tcacgcgcat tttggctgct ggtggcggct
     4141 ctattcctaa gttccatcgc catcaacgga gccatcacgc accttgcgcc gttattgacc
     4201 gatcgtggta tcagtgcgga gcactcggca ctggccgtct ccgttctcgg cgcggccagc
     4261 ctcaccggca ggctcgtaac gggcaatcta ctggaccgct gcttcggtcc agcggtttcg
     4321 tttgccctcc tggccggtgt cgcagccggg atctttttgc tcgcaaccgc ttccagcagt
     4381 ggaacagggc tgctggcggc ggccctgatc ggcttcggac ttggcgggga agcggatgtg
     4441 actccttatc tactgacccg ctacttcggt ctccgtatgt tttcgactct ctatgggctg
     4501 acgtggacag cctatgccat cgcgggagcg ataggcccca ttgtcatggg cagggtgttt
     4561 gacgtcaccc aatcgtacag tgcccttctt acggtgctat ccgcgctcac actgggtgcc
     4621 gcgatgctga tgttgttcct tcccgcttat cctctaaaag agcaacggga tcgagtggcc
     4681 ggaggtttga agtgcgcctg aaactgcttg gaatgatctt gggtggactg gctttcctgt
     4741 acttcgcggt agccgcttgc ctctacgtgg cgatgcggca gccaccggag cgctttggcg
     4801 ccatcatgtc gaaagttccg atggcagcga tgatggtcct gccattcaag tccctctgga
     4861 tgtctgcccg ggcgggacca cttgcggtgg gagataccgc tcccgatttt gcgctgccgg
     4921 ttctggaccg gagccgcacg gtcaggctgt cggaagaatg gcagacccgc ccggtggtcc
     4981 tgattttcgg cagctatact tgaccgccat ttcgccggga ggttcccgag ctcaatcggt
     5041 tataccagtc gtaccaggat cgcgtagcgt tctacgttgt ctacattcag gaggcccatc
     5101 ccaccgacgt ctggcagttg ccctccaatg tgacggatgg tgtgctgttc gcttcaccgc
     5161 gatcggacgg cgagcggacc tccaccgcac tggcgtgcgt acgcaatctc aaccttcggc
     5221 tcccggcggt tctcgatcac attgacaacc gaaccgagcg cgcctatacg gcttggcccg
     5281 atcgcatgta tctgatcgac agaaaaggcc gcgtagcgtt caagagcgcg cccgggcctt
     5341 atggtttctc aaccccggat ctcgagcagt ctcttcgaca gatcacaaca acgggaacct
     5401 gaccggcaaa cgggtgcatt cgcgtcgatc ggatagaatc ggagcatcat gcgtcgcctc
     5461 cggcttcttt ccgcgtttgc tataactgta accgtcgcgc tggcggccgg ccaggccggt
     5521 gccggggaga cttacgacgt tgttgttgtc agcggcagct cgggtggttt cggcgccgcg
     5581 ctggctgccg gacgcatggg gatgcgtgta gccctcatcg aagatacgcc ggtgctcggc
     5641 gggatgcttt ccaacgggat ctcgaacatc gacacttact ctttcgaatc gctgagtggc
     5701 atcttcgagg agttccgctc cagagtgaaa gaacactacc ggccgctgat ggctacagat
     5761 ccgatctttc gggcgcaacg gttgcgcgac tacaagccaa ggcaatcgaa tgtagccgcg
     5821 gaaggcggac ggtgggagcc gcatgttgcg gaccggatct tgaaagagat gatcgcgccg
     5881 gtgccgggcg tcactatctt ctacgatacg tgggcgacgg acgtggttct tcaggggaag
     5941 cacgccaccg gcgtcgtgac catgaacaag cgcggagaga agcgcgtctt caccggcaag
     6001 gtgattatcg atgctacgca cgaggccgac gtcgccgcct gggccggtgt gccttatcgg
     6061 gtgggccgtg aggcccgctc tcctctggag ccgcatgccg gagacatcta cttcttcaat
     6121 ggcaccggtg aaatcatgga gggcagcaca ggccgccagg accgggccat cgtgtcctac
     6181 ggattacggc tatgcatcca gcgctacggc ggagaggaaa catcgcacat ccttgcttct
     6241 cccccgccgg gctatgacag aagcaagtac acgcactccc cctattccgg cgagatcacc
     6301 atgccgaaaa gcaaagcgga gatgaacgcc aacccggttg gcaacgagat gcaggaagtc
     6361 aactgggtgt ggccggaggc gtcccgcgcc gaacggttgg aattgtacca gcagtataag
     6421 aatcacgcgc tggggtttct ctacttcctt cagcacgagc gcggattgaa gcacctggga
     6481 ttgccgaagg acgagttcgt ggacaacggc catgtcccgt atcgcatctt tgtgcgcgaa
     6541 gcccggcgcg ttgaaggcga agccaccatg accgaggccg atatcaatcc gtttctgacc
     6601 ggacgaggcc tgatcccggc gctgcgcaag gacagcatcg ccatcggaca ctatcccatc
     6661 gattcaaagc ccgtgcgcgg caagacagat atgtcgacac ccgaaaaagg cgccggagac
     6721 ttttttctgg ccaatgtggt tacaccgttc caggtgccct atggcgcaat agttccggcc
     6781 caggtagaag gcctcctggt tccggttgcg atatcggcga cgcacgtggc attctccgcg
     6841 attcgcatgg atccgacgtg gatggtgatg ggacaggcgg cgggggttgc ggcggcgctc
     6901 agcattcgcg agaacgtgat gccgcggcag ctcccgattg agcggttaca gcgcgagctg
     6961 atccggcaga aggtccgtct gctcttttac tgggacgttc ctctcgatca ccccgcattc
     7021 ggcgcgattc agtggctgag cgtgagcaag ggcttgcagg gatacccgga tcgcctgttc
     7081 cgcccggacc aacttctcag cagggcggag atgtccgttc tgatcgcgaa tgtcttcgat
     7141 ctatggccca gtgtcagtaa ctttcatttc acagacgttc cctggagcca ctgggccttc
     7201 cgcgaaatcg agacgctttt caacaaccgt ttgctcacgc ctttcggcgt ccggccccgc
     7261 tggccggacg ccggcggcta cgacccggtc cggaacgccg gattccggca agggcaaacc
     7321 ttcagcgcgt tcgaaccaga tcggcccgtg acatgggggg aaatggtcgg cgtgctccgc
     7381 acccttcaat cccgccagat acacccacct gtagaatggg tgaacggcat cgtcaaagat
     7441 tcggaattcg ccgtgagcct gcaaaagaaa gcgattttac ccgcagaggc gctcacccgc
     7501 gccgaggcgt gcatgctggt agcggctgtg agcgaacgca ggtagctacc agaaccacca
     7561 gcggcggttg ctcttgaccg gctccggcgg catgggcaag ggatctcccg tgagaacggc
     7621 tcgagggttc tcgatgcaga gcgcacgggc aactcgctca gcatagcgct ccgcaaggtg
     7681 cctgtagcct aagtccaacc gcgggggcct gtcgatgcag tcgtgagcgt cgctcgccaa
     7741 aaatgcgacc aggccacgtt caatcaaccc gtgagcaaag tccctggcat cccttccgaa
     7801 gcgccctaac agtgactgcg ccgtaacctg caaacagact ccgcgcgccg cccacgcggc
     7861 gagatcggcc gtcctgctat agagcagcga gttccgttcc ggatgcgtaa taaccggcac
     7921 catttgtgcc gcaagcatct cgttgaacac ttccccggtg ttctggaaaa tcag
//
LOCUS       DASTMK010000199         9685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_393439, whole genome shotgun sequence.
ACCESSION   DASTMK010000199 DASTMK010000000
VERSION     DASTMK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393439"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1365
                     /locus_tag="VFQ79_08695"
     CDS             253..1365
                     /locus_tag="VFQ79_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEU0139774.1"
                     /translation="MSQRERDRLVVLKKAQKKLITQKQAAVELSLTERHVRRLLVKLK
                     EAGDKAVIHGLRGRSSNRRVSEQTREEAVRILSQDVYQGFGPTLASEYLAKKHKLNVG
                     RETLRQVLMAAGLWRSRRQKVETVHQWRPRRSCRGELVQWDASEHDWLEGRGEKLYLI
                     HMIDDATSELTARFVRHDSSEENLRLLWSYLEKHGRPVAFYTDKASLFQTALKVRREE
                     KQRPREERERLPPTQIGRALRELAVTWIAAHSPQAKGCVERSFGTAQDRLVKGLRVAG
                     ARTLEQANAYLEEEFLPWWNQHLRVLPAHPADAHDPAASLSHVEMRQVGNDYTIRWDS
                     KLYRIDRTGVRPGMRGGLVRVEQRLDGTLAVRFRDR"
     gene            2188..2796
                     /locus_tag="VFQ79_08700"
     CDS             2188..2796
                     /locus_tag="VFQ79_08700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0139775.1"
                     /translation="MDRSNGYEGIAPEFLARRGSGRSTGIGVNQVRKWARTLPPGAAV
                     IDLGCGPGFPITEVLVAESLSTFGVDAAPSFVQAFQRNLPNTPVVCEAVQDSRFFDRT
                     FDGVLAWGLMFLLSPEDQRHLIERVAGILVPGGRLLFTSPAEPIVWNDAMTGLESRSL
                     GAAEYRRHMSAVGLSVASEYEDEGQNHYFDAFKDVHSLANPH"
     gene            complement(3070..4446)
                     /locus_tag="VFQ79_08705"
     CDS             complement(3070..4446)
                     /locus_tag="VFQ79_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0139776.1"
                     /translation="MNLNRRMFLQGGATAGLLRGQSRKPNIVMFLADDHGYFDSPVYG
                     NRSVGTPNMQRLASDGMTFTNMFSPSPTCVPSRSIVSTGLLPARNGAERNHSGVGPDI
                     RTLPTFMKSAGYHIAHFGKSHFQPKESFPDWEWVRSEIQGGRLNADLDTRALRSWIEA
                     RTNNTQPLCLFVCSHSPHVYWRGNEGYDAAKVDLPPTFVDTPETREARTQYYSDVTFA
                     DRQLGDAYDLVKQHLGGNTLFIYTSDNGAQWPFAKWSLYDAGIRLPFIASWPGVIRPG
                     SRTGAMASFCDLLPTFLDCAGAAAPSGIDGRSFAKVLRGTARTHRTAITATHSGDGDW
                     NVYPMRCLRTTRFKYILNLHPEFEYHTHIDLADPADGRSYFDSWVAKARTDAKAAAVV
                     RRYHERPAEELYDITADPHEMKNLAADTKYAGMLREMRERLRAIMNEQGDQGRVYGKP
                     RLISKAPQ"
     gene            4663..5103
                     /locus_tag="VFQ79_08710"
     CDS             4663..5103
                     /locus_tag="VFQ79_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0139777.1"
                     /translation="MPNTIRLHRVLRTTPEKLYRAFLESDALAKWLPPHGFTCKVHHM
                     DARVNGTYKMSFTNFTTGQGHSFGGTYLELIPGELIRYTDRFDDPNLPGDMQVTIQLK
                     RVSVGTELNIVQDGVPDVIPAEACYLGWQESLSQLAQLVEPDIP"
     gene            complement(5115..5657)
                     /locus_tag="VFQ79_08715"
     CDS             complement(5115..5657)
                     /locus_tag="VFQ79_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU0139778.1"
                     /translation="MGKPLIRTVGHSTRPVDALIEMLKAHGITRLIDVRTIPKSRHNP
                     QYNREALERSLRDAGIGYEHMKALGGLRHPRKDSPNLGWRNESFRGYADYMQTPEFES
                     ALNALTVAADREPLVIMCAEAVPWRCHRSLISDALVARGYRVDHIMSAAKADPHTMTS
                     FARVEGRSVTYPASTQERLF"
     gene            5762..7063
                     /locus_tag="VFQ79_08720"
     CDS             5762..7063
                     /locus_tag="VFQ79_08720"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0139779.1"
                     /translation="MVDRRSFVAAAAAGLGASRAAPGKPVREKPLVSTFWGTQHYDES
                     ERDQLAGVLQSRNPFRYYGHGSEAPQKAATFEKEFAARIRTRFALAVATGTAALDTAM
                     AALDIGPGDEVILPAWTFVSCYNTIVLAGALPVFAEVDESFNIDPDDIERRITPATRL
                     IMAVHLRGNACDMDRIMAIARKRNIKVLEDAAQSMGASYNGRPLGSIGDVGMFSLQPS
                     KPIAAGEGGIVTMNDPLLYERAVRYHDVGGCRPYHEKILGTPALEGFPGTNLRMNEWT
                     AAVALAQLRKLDGILAAVRAHSRRVYDGIRDLPGIRFRLRPDPEGELGAVVFIRFDDK
                     PRCDRFAAAMKAEGVPVTRPGSAAILPAVPFIEKKIAPRRNWPTFATGRGRSISYGAG
                     ICPRSIDIVARFAGPSIDPKYTRADVDDIVAAVRKVYPTVR"
     gene            complement(7077..>9685)
                     /locus_tag="VFQ79_08725"
     CDS             complement(7077..>9685)
                     /locus_tag="VFQ79_08725"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0139780.1"
                     /translation="VAVKVLKPGADSSFVLRRFSHEREIVANLHHPNIARFLDGGSTD
                     DGLPYFVMEFIEGRPIDEYCDARGLSTDDRLRLFVTLCSVVEFAHQKGVIHRDIKPNN
                     VLVAGTGVLKLLDFGIAKILDPDFSSQPISVTGSMMRLMTPDYASPEQIKGEPVTAAT
                     DVYALGVVLYELLTGRRPYHLKSGTPLELMQAICEKEPEKPSTAVAGGGSTTATAAIP
                     PGQPPPPSVEAHKLEGDLDAIILKAIRKQPGERYASVKTLQEDIERHLNGGTVLARLE
                     RPRGTAAPETGNHRTLWIAIAIAAVLLVFALAPLVRSYLASTRKVELPFVTPVTTFPG
                     VETQPYFSPDGKKIVYVWRREESRNSDIYVRTLATGEQLRLTTDPAEDLSPVWSPDGK
                     RIAFLRTSPKETAIFVSPAGGGVHGKIAEVYPTRFEAVGRHLDWSPDGKLLAVADKSA
                     PEEPFSIFLVSVADGGKTRLTHPSEKMLGDSNPAFSPDGKWVSFLRAPSSGVADVYLA
                     SLENGETRRITFDNRSIQSQTWDAQGQSILFSSDRTGALNLWRISVQGHAAPSRLPGM
                     GENATEPVYSKDGKMLAYSQISVDANIWRVPLSGGPAQSHIVSTYYDSSPSFSPDGRR
                     IAFRSNRSGSNEIWVSDADGRNTLQLTRFGGPLTGTPRWSPDGSWIAFDSRPQGQSDI
                     FLIRPEGGNTHHLTREPSEDVVASWSRDGKWVYFASNRSGQWQGWRTSIDGVQKQQMT
                     QNGGFGAMESADGRYIYFAKGPGEPGLARLTLASGQERLVLPSLKAGYWGYWALTRDR
                     IFFVDQDANDPQAAIYSVNLDGGEKRLVFKMEKPPSIADSAFAISPDQQWILYTQIDH
                     SGGDIMLADYSK"
BASE COUNT         1838 a         2983 c         3031 g         1833 t
ORIGIN      
        1 gtccttggcc aagcgagatc tggccaacgg ttgccctcac aatgtggaag agttgatgga
       61 cgaccttgtc cgctccatcg aaagaatccg ccgatcgccc gccaaactcc gaggttgcat
      121 caagcagtcg aaacttcccc ccttttttgc gttaactgtt gcattattta tgcatagatc
      181 tattagtgcc aagtagaaat gtcctgttga cagggtcacg ctggggagtt ggacgaacag
      241 ggagttctga caatgagcca gcgggaacga gaccgcctgg tggtgctaaa gaaagcacag
      301 aagaagctga tcacacagaa gcaggcggcg gtcgagctga gcctgacgga gcgtcacgta
      361 cgacgactgt tggtaaagct gaaggaggcc ggggacaagg cggtgatcca cgggctgcga
      421 gggcggtcct cgaaccggcg ggtgagcgag cagacgcggg aggaagcggt gcggatcctg
      481 tcgcaggatg tgtaccaagg cttcgggccg acgctggcga gcgagtacct ggcgaagaag
      541 cacaagctca atgtggggcg ggagacactg cggcaagtcc tgatggccgc cggactgtgg
      601 cggagccggc ggcagaaagt ggaaacggtg caccagtggc ggccgaggcg aagctgccgg
      661 ggcgagctgg tgcaatggga cgcctccgag cacgactggc tggaagggcg tggcgagaag
      721 ctgtatctga tccacatgat cgacgacgcg acgagcgaat tgacggcgcg attcgtgcgt
      781 cacgactcga gcgaagagaa cctgcgtctg ctgtggagct acctggagaa gcacggacgg
      841 ccggtggcgt tttacaccga caaggcgagt ttgtttcaaa ccgcgctgaa ggtccggcgg
      901 gaggagaaac aacggccgcg cgaggagcgc gagcggttac cgccgacgca gatcgggcgg
      961 gcgttgcggg agttggcggt cacctggatt gcggcgcact cgccacaagc gaaggggtgc
     1021 gtcgaacgga gcttcggcac ggcccaggac cggctggtca aaggactgcg cgtggcggga
     1081 gcgcgcaccc tcgagcaggc caatgcctac ctggaggagg agtttctgcc ctggtggaat
     1141 cagcatctga gggtgctgcc ggcccatcct gccgacgcgc acgatccggc ggcctccttg
     1201 agccatgtcg agatgcgcca ggtaggcaac gattacacga tccgctggga cagcaaacta
     1261 tatcgcatcg accggaccgg cgtgcgtccc ggtatgcggg gtggcctggt ccgtgtcgaa
     1321 cagcgtctgg acggcaccct cgccgtgcgt ttccgagatc gctaggtgcg gatcgaggaa
     1381 ggtgtgcggc gcgagaaaac ggtgccgcca caacggccag cggccaagca cagcccatcg
     1441 cgccacaaac ctaccgaggc ggcccggagc agcctcgatc gtctgtttcg ctcgcccagc
     1501 cggccaatct ggacggcggc cgagatcgat cgcacccgca cgcgcgatcg cctcgattaa
     1561 agctccctgc ggagcgccgg gcaaaagcag cccggcggtc ttcgcgcttt ctatatccga
     1621 agaccgctgc tccagagctt caccaaagca gatatccgtg caaaaaccaa aaccctcgga
     1681 acaggtcccg ctgtggaccc attgaccggg ggttgggtgc catctgcttc ggagccagcc
     1741 ccgtcgtagc ggtcttcgga tctgccccgg ggagcgcgac tccccaacaa catcgatctc
     1801 aacttaaacc cgctctcgcg gtgtggctct caataccgga catttctact tggcagagaa
     1861 ccggacattt ctatttggcc ttgacatggg attgggtagc ttaccgaaag taggttgttg
     1921 ctgccctgga gcaattcgag cgtccgcgcg ccctgtgacg ccccctggat ctggcggttt
     1981 cgggcagtgg gcagccagtt ggacggaaag tcgccgccga cgttaccagt cgtcggccca
     2041 cgtgagagca tcaggcgtta cccgtcaagt tgacgccggt tatgcgaaat tctggatgcc
     2101 acgcgctgca ggcggagtcc agcgttccgg ggttatgtcg aacaggcact cgccgggcgc
     2161 gaacccaagc tgatactgtg aggcgtgatg gaccgatcga atggatacga aggaattgcg
     2221 ccggagtttc tggcccggcg cggcagtggc cgttcgaccg gcatcggcgt gaaccaggtg
     2281 cggaagtggg cccgaacgtt accacccgga gccgctgtca tcgacctggg ctgcgggccc
     2341 ggttttccga ttaccgaggt tctggtcgcc gaaagcctta gcacgtttgg agttgatgca
     2401 gcaccatctt tcgtccaggc tttccaacgc aatctcccga acacacccgt cgtttgcgaa
     2461 gccgtccagg attcgagatt cttcgatcgc actttcgatg gtgtcctggc gtggggacta
     2521 atgttcctgc tttcgcccga ggaccagcgc cacctcatcg agagggtcgc cgggatactg
     2581 gtgccagggg gccggctgct gtttacgtct cctgcggaac caattgtttg gaacgatgcg
     2641 atgacggggt tggaatcacg ctcccttgga gccgcggagt accgtcggca catgtcagca
     2701 gttggtctct cggtcgccag tgagtatgag gacgaaggcc aaaaccacta tttcgatgcg
     2761 ttcaaggacg tgcattcttt agccaatcct cactgatgca accctggagg cgatccattc
     2821 tgacggttgt cgccatctca gagatgtgca ctggctggat tgctacggga tcacgcgcca
     2881 cccggaacca gcccggtgaa gtggctgagc gcgactctac tcaagccgcc tgcgccagcc
     2941 acaagggagt tcttccccaa gtgcactgtt atcggtgcgg atggagggaa gccattaagt
     3001 gcttcgaatc tgagattggt gagcgccgga gcggccattg ccgctgcgcc caacagaacg
     3061 ctcgccgctc tattgcggcg cctttgaaat cagccgcggc ttgccgtaca cgcggccttg
     3121 atcgccttgc tcattcatga tggcgcgcag cctctccctc atttcgcgca acattccagc
     3181 gtacttcgtg tccgcggcga ggttcttcat ctcatgggga tcggcggtaa tgtcgtacaa
     3241 ctcttcggcc gggcgctcgt gataacggcg cacgacggcc gctgctttcg cgtcagtcct
     3301 ggctttggcc acccaggaat cgaagtacga gcggccgtcg gccgggtccg cgagatcgat
     3361 atgcgtgtgg tactcgaatt ccggatgcag attgaggatg tacttgaacc gcgtggtgcg
     3421 caggcagcgc atcggataga cgttccagtc gccgtctccc gagtgcgtcg cggtgatcgc
     3481 ggtccgatgc gtgcgcgccg tgccccgcag tactttcgcg aacgatcgcc catcgatgcc
     3541 ggagggcgct gcggctccgg cgcagtcgag gaaggttggc agcagatcgc agaacgaggc
     3601 catcgcgccg gttcgcgatc ccgggcggat cactccaggc catgacgcga tgaacggcag
     3661 ccggatccct gcatcataga gcgaccactt tgcgaatggc cattgcgcgc cattgtcaga
     3721 tgtgtagatg aacagcgtat tgccgcccag gtgctgtttc accagatcgt aggcatcacc
     3781 aagctgacgg tcggcgaagg taacgtcgga atagtactgc gtgcgggctt cccgggtctc
     3841 tggcgtatcg acgaatgtgg gcggcaggtc caccttcgcc gcgtcgtagc cttcattgcc
     3901 gcgccagtac acatgcgggc tgtgagagca cacaaacagg cacagcggtt gtgtgttgtt
     3961 cgtgcgcgcc tcgatccagg aacgcaaggc gcgtgtatcc agatccgcat tgagccgtcc
     4021 accttgaatc tcgctgcgca cccattccca gtccggaaag ctctccttgg gctgaaaatg
     4081 gctcttgcca aagtgcgcaa tgtgatagcc cgccgacttc atgaacgtgg gaagcgtgcg
     4141 gatgtccgga ccgactccac tgtgattgcg ctccgctcca ttgcgcgcgg gcagcagccc
     4201 cgtggagacg atcgagcgcg atggcacgca ggtcggcgac ggcgagaaca tgttcgtgaa
     4261 cgtcatcccg tctgacgcaa gacgctgcat gttcggcgtg ccgaccgacc tgtttccata
     4321 aaccggcgaa tcgaagtatc cgtgatcgtc ggccagaaac atcacgatgt ttggcttccg
     4381 cgactggccg cggagcaatc ccgcggtggc gccaccctga agaaacatgc gtctattgag
     4441 attcatcgcc caatcccccg attactctcg aacgcaccgc gctcaggcca gtccgcatcg
     4501 aaatcatgct gccagccatt atgcgtgtta ggcacggccg ccgaagaccg gtgcctctcc
     4561 ggattactat tgtggacata ttccattgcg gggctgtcga atttgcggct gttcgatcga
     4621 ctaacaggca ggatgtctct taccctggaa aggaccaatc acatgcccaa tacgatacgc
     4681 ctgcatcgcg tactgcgcac cacgccggag aaactctacc gggcgttcct cgaatccgac
     4741 gccttggcca agtggctgcc gccgcacggt ttcacctgca aggttcacca catggacgct
     4801 cgcgtgaatg gcacgtacaa aatgtcgttc acgaacttta caacaggtca gggccactcg
     4861 tttggcggaa cgtacctcga gttgattccg ggtgagctca tccggtacac cgaccggttc
     4921 gatgatccga atctgcccgg tgatatgcag gtaacgatcc aactgaagcg ggtttcggtg
     4981 ggaaccgaac tcaacattgt tcaagacggt gtgcccgatg tcattccggc ggaggcatgc
     5041 tatctggggt ggcaggaatc cctttcccaa ctggcgcagc ttgtggagcc ggacatcccc
     5101 tagcagtcct gatcctaaaa cagtcgttcc tgtgtactgg caggatacgt aaccgatctc
     5161 ccttccaccc tcgcaaatga cgtcatggtg tgcgggtccg ccttggccgc gctcatgatg
     5221 tggtccaccc gataaccacg cgccaccagg gcatccgaaa tcagcgagcg gtggcagcgc
     5281 cacggcacag cctcggcgca cattatgacg agaggttcgc gatctgccgc cacggttaat
     5341 gcattcagag cagactcaaa ctcgggcgtc tgcatgtagt ctgcatagcc gcgaaacgac
     5401 tcgttgcgcc aacccaggtt tggcgaatcc ttgcgaggat gccgtaatcc cccgagggct
     5461 ttcatgtgct cgtagccgat gccggcgtcg cgcagcgacc gctccagcgc ctcgcgattg
     5521 tactgcggat tatggcgcga tttgggaatc gtgcgaacgt cgatgagccg cgttatgccg
     5581 tgggccttca gcatttcgat cagtgcatcc accggccgcg tggaatgccc gaccgtccgg
     5641 ataagcggct ttcccatatc gccattttcg atcacggggg cttgcttccg ctcatggttg
     5701 atgccgtgac cggccaatgt gaggtaccct gctgctgaat gcctggaaca aggagactga
     5761 cttggtagat cgcagaagtt tcgttgccgc ggcggccgcc ggcctggggg catcgcgcgc
     5821 cgcgccgggc aagcccgtgc gggagaagcc cctggtttcg accttctggg gcacgcagca
     5881 ttacgacgaa agcgagcgcg accaactcgc cggcgtgctc caatcgcgca acccgttccg
     5941 ctactacggg cacggcagcg aagccccgca gaaggcggcg accttcgaaa aggaattcgc
     6001 cgcgcgcatc aggacgcggt tcgcgctcgc ggtcgcaacc ggcacggcgg cgctcgacac
     6061 ggcgatggcg gcgctcgaca tcggcccggg tgatgaagtg atcctgccgg catggacgtt
     6121 cgtctcctgc tacaacacca tcgtcctggc cggcgctttg ccggttttcg ccgaggtgga
     6181 cgaatccttt aacatcgacc cggacgacat cgagcggcgc atcacgcccg ccacgcggct
     6241 catcatggcg gtgcacctgc gcggcaacgc ctgcgacatg gaccgcatca tggccatcgc
     6301 acggaagcga aacatcaaag tgctggagga cgcggcgcag agcatgggcg cctcttacaa
     6361 cggccggccg ctcgggtcga tcggggacgt gggaatgttc agccttcagc ccagcaagcc
     6421 cattgcggcc ggcgaaggcg gcatcgtgac gatgaacgac ccgctgctct acgagcgcgc
     6481 ggtccgctat cacgacgtcg gcggctgccg cccctatcac gagaagatcc tcggcacacc
     6541 cgcgctcgaa ggcttccccg gcaccaacct gcgaatgaac gagtggacgg cagcggtggc
     6601 cctggcccaa ctgcgcaaac tcgacggcat cctggccgcc gtgcgggcgc actcgcggcg
     6661 cgtctacgac ggcatccgcg acctgcccgg catccgcttc cgcctgcgcc ccgatcccga
     6721 gggagagctc ggcgccgtcg tcttcatccg gttcgacgac aagccgcgct gcgaccgctt
     6781 cgccgcggcg atgaaagccg agggcgtgcc cgtcacgcgc cccgggagcg ccgcgatcct
     6841 gccggccgtt cccttcatcg aaaagaaaat cgcgccgcgc cgcaactggc cgaccttcgc
     6901 caccgggcgc ggcaggtcga tttcgtacgg tgccggcatc tgcccgcgca gcatcgacat
     6961 cgttgcgcgc ttcgccgggc cttcgatcga cccgaagtac acacgcgccg acgtagacga
     7021 tatcgtcgcc gccgtccgca aggtctatcc gactgtgcga tgaggcagcc ccgctcctac
     7081 ttcgagtaat ccgccagcat gatgtcgccg ccgctgtggt cgatctgggt gtacaaaatc
     7141 cactgctggt cgggtgaaat ggcgaaagcg gaatcggcga tggaaggagg tttttccatt
     7201 ttgaagacca gcctcttctc tccaccgtcg aggttcacgg aatagatagc ggcttgcgga
     7261 tcgttcgcgt cctggtccac gaagaagatc cgatcgcggg tcagggccca gtagccccag
     7321 tatccggctt tgagcgaagg cagtaccagg cgctcctgtc ccgacgccag agtgagccgc
     7381 gccaggcccg gttcgccagg accttttgcg aagtagatat accggccatc ggcggattcc
     7441 atcgcgccga atccgccgtt ctgcgtcatc tgctgcttct gtacgccgtc gatggaggtc
     7501 cgccacccct gccattgccc ggaacggttc gatgcgaaat acacccactt tccgtcgcgg
     7561 gaccaactgg caaccacgtc ttccgacggt tctctggtca gatggtgtgt gttgccgcct
     7621 tcgggccgta tcaggaagat atcggactgc ccttggggcc gtgaatcaaa cgcgatccac
     7681 gacccgtcgg gggaccatcg cggcgtcccg gtcagtggcc cgccgaaacg cgtcaattgg
     7741 agtgtattgc gcccgtccgc atcgctcacc caaatctcat tgctgcccga gcggttggag
     7801 cggaaggcaa tgcgccggcc atccggtgaa aacgacgggc tcgaatcgta atacgtggag
     7861 acgatatggc tttgcgccgg gccaccggac agaggcacgc gccagatgtt ggcgtccacc
     7921 gaaatctggg aataggccag catcttgccg tccttcgagt acaccggctc ggtggcattc
     7981 tcacccatgc cggggagtct cgaaggagcc gcgtgtccct gcacggagat ccgccacaga
     8041 ttcagagcgc cggtacggtc cgaggagaac aggatgctct gcccttgcgc gtcccacgtc
     8101 tggctttgga tcgatcggtt gtcgaacgta atccggcgtg tctcgccatt ctcgagcgag
     8161 gccaggtaaa catcggctac accgctgctc ggcgccctca ggaaggagac ccatttcccg
     8221 tcgggcgaga aagcaggatt gctgtcgccg agcatcttct cggacggatg agtcagtcgc
     8281 gtcttaccgc catccgcaac cgagaccaaa aagatactga acggctcctc gggcgcggat
     8341 ttgtcggcaa ccgccagcaa cttgccgtcg ggagaccagt ccaggtgccg tcccactgct
     8401 tcaaaccgcg tcgggtagac ctcggcgatc tttccatgaa ctccgccgcc agcgggcgac
     8461 acgaatatgg ctgtttcctt cggcgatgtt cgcaggaagg cgatccgttt gccgtcgggc
     8521 gaccacaccg ggctcaagtc ctccgccgga tcggtggtca gccgcaattg ctcgccggtc
     8581 gcgagggttc gcacgtaaat gtcggagttc ctgctttcct ccctgcgcca gacataaacg
     8641 atctttttgc cgtcgggcga aaagtagggc tgtgtttcca cgcccggaaa ggtcgtcacc
     8701 ggcgttacga aaggtaattc cactttccgg gtggaggcga ggtaggaacg aaccagcggt
     8761 gccagcgcga ataccagcag cacggccgca atcgctatgg ctatccacaa cgtgcggtgg
     8821 ttcccggttt ccggcgccgc ggtgccgcgc ggccgttcga ggcgcgccag cacggtgccg
     8881 ccgttcaggt gtcgctcgat gtcctcctgc aatgtcttta cggaagcgta ccgctcaccc
     8941 ggctgctttc gaatggcctt cagaatgatc gcgtccaggt cgccttcgag cttgtgggcc
     9001 tcgacggacg gcggtggcgg ctggccgggt gggatggccg ctgttgccgt ggtgcttcca
     9061 cctccggcga cggccgtgct gggcttttcc ggctcttttt cgcagatggc ctgcatcaac
     9121 tcgagcggcg ttccgctctt caggtggtac ggccggcgcc cggtaagcaa ctcgtacaaa
     9181 accacgccga gcgcgtagac atcggtcgcg gccgttaccg gctctccctt gatttgctcg
     9241 gggctcgcgt aatccggggt catcaggcgc atcatggaac cggtaacgct gatcggctgg
     9301 gacgagaagt cggggtccag gatcttggcg ataccgaagt cgagcagctt gagaacgccg
     9361 gtgccggcca ccaggacgtt gttcggcttg atatcgcggt ggatgacgcc tttttgatgc
     9421 gcgaattcga ccaccgagca caaggttacg aacagccgca gccggtcatc cgtggagagg
     9481 ccgcgggcgt cgcaatactc atcgataggc cggccttcga tgaattccat cacgaagtag
     9541 ggcaatccat cgtcggtact gccgccgtcc aggaacctgg cgatattcgg gtgatggaga
     9601 ttcgctacaa tttcgcgctc atggctaaag cggcggagca cgaaactgct gtcggcgccc
     9661 ggcttcagta ccttcacggc caccg
//
LOCUS       DASTMK010000200         5456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_106049, whole genome shotgun sequence.
ACCESSION   DASTMK010000200 DASTMK010000000
VERSION     DASTMK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106049"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1440)
                     /locus_tag="VFQ79_08730"
     CDS             complement(28..1440)
                     /locus_tag="VFQ79_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0139781.1"
                     /translation="MKMTEGDVQMLALAGESETIEFKRSTGQLPRAGETLCGFLNGKG
                     GHVVFGVAPDGQMVGQSVSDATMQDVAQMIRRFEPPASVDIERVRLGSGQEVLVLRVS
                     ISGGETTPFTYDGRPYQRIGTTTSVMPQQTYQRLLLDRAHARQRWENLPSSFEIEDLD
                     SGEIARTREGAIAAGRLDPLMSGADEDFLERVGLRANGRILNAAVVVFGRRFLPDYPQ
                     CQLRLARFRGRNKTEFKDQRQVTGHALQLMSEAMEFLSRHLPIAGRVEPGVFERVDEP
                     LFPPIALREALVNAFCHRDYAQVGGAVSIGIYDDRLEVWSDGTLPFGLSPDDLKRDHL
                     SRPRNPLIAEVFYRRGLVERWGRGTQRLVELCVRAGHPEPEFLESGGAVCVRFLPSGY
                     IAPHRVAHDLTQRQRKLLQALASKEARSFSQLRAEVAPETPERTLRDDLSHLKRLSLI
                     DSKGMGRGAVWFLREPNEAE"
     gene            complement(1861..2223)
                     /locus_tag="VFQ79_08735"
     CDS             complement(1861..2223)
                     /locus_tag="VFQ79_08735"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0139782.1"
                     /translation="MRSSFRVERRTEARLLCSDLVTLSWRDPTGKSRQEIVNLEDISR
                     SGACLQSEVAVEEKTKVEITMGAITLRGQVRYSRRDELGYFIGVLFESGSRWSKEAYE
                     PKHLFDPRTLVRKQRNGS"
     gene            complement(2274..2714)
                     /locus_tag="VFQ79_08740"
     CDS             complement(2274..2714)
                     /locus_tag="VFQ79_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139783.1"
                     /translation="MKTIIFAIGNSLRRDDGAAHRVLELLEPHPGVLTRSLLQLAPEQ
                     SEEIAPARMVVFVDADIEPGPARIERLPRGGPPPRSPLAHSLRPAEIVALARSLFHFT
                     GQAYVCHVPGADFSEGEGLSGTASRNAIRAAELLRDLLYTPESR"
     gene            complement(2750..4174)
                     /locus_tag="VFQ79_08745"
     CDS             complement(2750..4174)
                     /locus_tag="VFQ79_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEU0139784.1"
                     /translation="MAERIIINPVTRIEGHAKISIYLDDSGQVDSAQFHVVEFRGFEK
                     FCQGRPFHEMPGLMSRICGICPVSHILASAKAGDHLLGVQVPPAAEKQRRLINYAQLL
                     QSHALSFFHLSAPDLLLGFDSPVENRNVFGLAEKDPQTARRGIRLRQFGQRIVELLGG
                     KKIHPAWATPGGVLSAVSADTRDEILARVPEAIDTLQKALGTMKSIMDTFHDEIEHIG
                     NFPSLFLGTVSREGELEYYNGLIRIVDGEGNIVADGLDPLRYHTFLGEASEEWSFMKF
                     PYYKPLGYPGGMYRVGPLARLNVARWAGTSLADRELREFKQRIRESVCQSFHYHMARL
                     IEMLHAVERIEEVASDAELLDTHVCARAGQNRNEGIGSCEAPRGTLFHHYTVDDHGLI
                     QKANLLIATAQNNLAINRAVEQTARRFVSGNTMTEGMLNRVEAAIRCFDPCLSCSTHA
                     IGQMPLVVELKSSRGDVLSRIEQG"
     gene            complement(4179..4727)
                     /locus_tag="VFQ79_08750"
     CDS             complement(4179..4727)
                     /locus_tag="VFQ79_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HEU0139785.1"
                     /translation="MGAVNGNPKPTLATVWLGGCSGCHMSFLDLDERLLDLASKMELK
                     ASPLTDYKDFPEVDVVLVEGAVANSEHLEHIRTVRERSKILVSFGDCAVTGNVTAMRN
                     AFGVDDVLNTAYRDNSTVIIGLPVGNHIVPRLLDRVRPVHEVVKVDYFLQGCPPSADA
                     IFEFVSALIEGREPSLEGRKFG"
     gene            complement(4715..5431)
                     /gene="hoxU"
                     /locus_tag="VFQ79_08755"
     CDS             complement(4715..5431)
                     /gene="hoxU"
                     /locus_tag="VFQ79_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HEU0139786.1"
                     /translation="MSVRTLEIDGKAVTGVAGQTIFSVAWDNGIHIPRLCHVGGVSDV
                     GACRLCMVEVEGQRKLLAACMTPVEEGMVIRTDTERLVAHRRMIVELLLAERNHVCAV
                     CVVNGACELQDLAASLGIDHVRLRYRFPALSVDTSHERFGLDHNRCVLCSRCIRVCDE
                     VEGAHTWDFSGRGANSLLISDLGEPWGESESCTSCGKCVQVCPTGALFAKGKMVSEMR
                     KDRRFLKYIVTAREKHEWVR"
BASE COUNT         1145 a         1692 c         1540 g         1079 t
ORIGIN      
        1 aaggcggaat gaggcggtgg cgccgcctta ttccgcctca ttgggttccc tcaaaaacca
       61 gaccgcacca cgtcccatac ccttggagtc gatgaggctc agccgcttca agtgcgaaag
      121 gtcgtctcgg agggtccgct ccggcgtttc gggtgccact tcagcgcgca actgactgaa
      181 agatcttgcc tctttgctgg cgagcgcctg aagcaatttc cgctgccgct gggtgagatc
      241 gtgtgcaacc cggtggggag caatgtagcc gctgggaagg aagcgcacgc agacggcccc
      301 tccgctctcc aagaactctg gctctggatg cccggccctg acacacagtt cgacgagcct
      361 ttgagtaccc ctgccccagc gctcgaccag gccacggcga tagaacacct cggcgatcag
      421 gggatttcgc ggccgtgaaa gatgatcacg tttcaagtca tctgggctta gcccgaaagg
      481 caaagtgccg tcgctccaga cttccagccg gtcgtcgtag atgccaatac tgaccgcgcc
      541 gccgacctgc gcgtagtccc ggtggcagaa tgcgttgacc aatgcttctc gaagcgcaat
      601 tggcggaaac agaggctcat ccactcgctc aaacacaccc ggctcgaccc ttccagcaat
      661 cggcaagtgc cgcgaaagga actccatcgc ctcggacatc aactggagag catgcccagt
      721 gacctgacgc tgatccttga actcggtctt gttccttccc cgaaaccggg ccaaacgcag
      781 ttggcattgc ggatagtcgg ggaggaaacg gcgtccaaag acgacaactg ccgcattgag
      841 aatcctcccg tttgcacgaa gccctacccg ctcaaggaaa tcctcgtcgg cgccggacat
      901 gagaggatcc aaccgcccgg cagcaatagc cccctccctg gtgcgcgcga tttcgcccga
      961 atcgagatct tcgatttcaa acgatgacgg caggttctcc cagcgctgcc gggcgtgcgc
     1021 gcgatcgagc agcaggcgtt ggtaggtctg ctggggcatt accgatgttg tcgtgccaat
     1081 gcgctggtag ggccgtccgt catacgtgaa gggggtcgtc tccccgccac taatcgaaac
     1141 acgtagaaca agaacctcct gcccagaacc cagtcgcacc cgttctatgt caactgaagc
     1201 gggtggctca aatcgccgga tcatctgcgc cacatcctgc atggtcgcgt ctgaaactga
     1261 ctgacctacc atttgaccat cgggggcgac gccgaagaca acgtgacccc ctttgccgtt
     1321 cagaaacccg cagagggtct ccccagcacg cggcaactgg ccagtcgaac gcttaaattc
     1381 aatggtctcg gactcgccag cgagcgcgag catctgaaca tcgccctcgg tcatcttcat
     1441 ctatcatgcc ctgattcgag agaagactga aatgtgcggg acgcgctcca gcgattcgtt
     1501 tgctctccgg gagccatgag cgtattctgg gttcgagggc tccttgggga aacgctttct
     1561 ggactcgaaa ccgcagccca acttggctgg cttcgttcga cttccagacg ggtgctccca
     1621 ggaagaaaag tgctaacaaa ttgctaacag gagatccaga aacaaccaga atcaaccaag
     1681 acatcctcga cgtaacttgt tgacggctct gaacagcgac acccaaccat aacgatgtgt
     1741 taaactcata acccaaaggt cgaaggttca aatccttccc ccgcaaccaa taaaacgtca
     1801 ggttgcgccg cttcaggctc tctgcaaact cccctttaac aagctaggcg aattgccgga
     1861 tcaggagccg ttgcgctgtt tccggacgag ggttctgggg tcgaagagat gtttaggctc
     1921 gtaggcttcc ttggaccacc tggatccgct ctcaaacagt actccgataa agtagcccaa
     1981 ctcatcccgc cggctgtagc ggacctgacc gcggagagtg atggcaccca tggtgatctc
     2041 aaccttggtt ttttcctcga cagccacttc ggattggagg caggcgccgg accgcgagat
     2101 atcctccaaa ttcacgattt cctgtctgct tttgcctgtc ggatcccgcc aggacaaggt
     2161 tacaaggtcg gagcatagca ggcgcgcttc cgtacgtctc tccacccgga aagagctacg
     2221 cacaggctgt tgtccttcca ttcgaattcg ccaaagcaga cggtgatttt agcctagcgg
     2281 gattcggggg tgtaaagcaa gtcgcgtaac agctccgctg cgcggatggc gttcctggag
     2341 gctgtgccag acaacccctc accctccgag aaatcagcgc cggggacgtg gcagacatag
     2401 gcctggcccg taaaatgaaa taggctccgc gccagcgcga cgatttcggc gggacgcagg
     2461 gagtgtgcca gtggacttcg gggaggaggg ccgcctcgcg gcaggcgttc gatgcgggcg
     2521 ggaccgggtt cgatgtcggc atcgacgaag acaaccatcc gcgccggtgc gatttcttcg
     2581 gactgttcgg gagcgagttg cagaaggctg cgggtgagga cgccggggtg cggctccaac
     2641 aactccagca cccggtgggc cgctccatcg tcgcgcctga gcgagtttcc gatcgcgaag
     2701 atgatcgtct tcatatcgaa ccttgggccg ccgcagcccg ccggggtgtc tatccctgct
     2761 caatacgcga taggacatcc ccccttgagg atttcagttc aaccacaagc ggcatctgtc
     2821 cgatcgcgtg agtagagcag gacagacacg gatcaaaaca gcggatggcc gcttccacgc
     2881 ggttcaacat accctccgtc atcgtattgc cggaaacgaa ccgccgggcc gtctgttcca
     2941 ccgccctgtt gatggcgaga ttattctgag cggttgcgat gagcaggttg gccttctgaa
     3001 tcaagccgtg atcgtccacg gtgtagtggt gaaaaagcgt gccgcggggg gcttcgcaag
     3061 atccgattcc ttcgttacgg ttctgccctg ccctggcgca aacatgcgta tccaataatt
     3121 cggcgtcgct ggcgacctct tcgatgcgct cgacggcgtg cagcatctcg atgagccgcg
     3181 ccatatggta gtgaaacgac tggcacaccg actcccgaat gcgctgctta aattctcgaa
     3241 gctcgcggtc ggccagcgat gttccggccc accttgcgac attcaaacgc gccagcgggc
     3301 ccacgcgata catgccgccc ggataaccga gcggtttgta gtaggggaac ttcatgaacg
     3361 accattcctc gctcgcttcc ccgaggaagg tgtgatagcg caggggatcc aaaccgtcgg
     3421 caacgatatt accttcgcca tcgacgatac ggatcagccc gttgtaatac tcgagttcgc
     3481 cttcccggct caccgtgccc agaaagaggc tcgggaagtt gccgatgtgc tcgatctcat
     3541 cgtggaaggt atccatgatg gatttcatcg tgcccagcgc tttttgcagg gtatcgatag
     3601 cttcgggaac ccgggccagg atttcatccc gggtgtcagc ggaaaccgcg ctcagtacac
     3661 cgccgggtgt ggcccacgcc gggtgaatct ttttgccgcc cagcaactcg acaatgcgct
     3721 gtccaaactg ccgcaggcga atgccacgcc gcgccgtctg cgggtctttt tcggcgagtc
     3781 cgaacacatt tcgattctcg acgggtgaat cgaaaccaag cagcaggtcc ggcgccgaga
     3841 ggtgaaagaa gctgagcgcg tgcgattgca ataattgcgc gtagttgatg agccggcgct
     3901 gcttttccgc cgccggtgga acctgaacgc ccaacaggtg atctcccgcc ttggcgctgg
     3961 cgagaatgtg gctgaccgga cagatgccgc agattcggga catcaggccc ggcatctcat
     4021 gaaacgggcg gccctggcag aacttctcga atccacggaa ttccaccaca tgaaactgtg
     4081 cggaatcgac ctgtccggag tcgtccaggt agatggaaat cttcgcgtgc ccctcaatgc
     4141 gggtcaccgg gttgataatg attcgttccg ccattgtttt aaccgaattt tcgcccttcc
     4201 agcgatggtt cacggccctc gatcagcgcc gacacgaact cgaagatcgc atccgccgaa
     4261 ggcgggcacc cctgaaggaa ataatccacc ttcaccactt catgcaccgg acggacgcga
     4321 tcgagcagcc tggggacaat gtgattccct acggggaggc cgatgatgac ggtcgagtta
     4381 tcgcgataag ccgtgttcaa aacgtcgtcc accccgaagg cgttgcgcat cgcggtgaca
     4441 ttccccgtta ccgcgcagtc gccgaacgat accagaattt tggaacgttc gcgcacggtg
     4501 cgtatatgct ccaggtgttc cgagttcgcc accgcgccct cgaccagcac gacgtcaacc
     4561 tccggaaaat ctttgtagtc ggtgagcgga gatgccttca gctccatttt ggatgccaga
     4621 tcgagcagcc gctcgtccag atcgaggaac gacatatggc atcccgaaca gcctcccagc
     4681 cataccgtcg cgagagttgg tttggggttg ccgtttaccg cacccattcg tgtttctccc
     4741 tggcggtcac aatgtacttg aggaatctcc ggtccttgcg catctcgctt accatcttgc
     4801 ctttcgcaaa cagagcaccg gtcgggcaca cttgcacgca cttgccgcac gacgtgcagc
     4861 tttccgactc gccccagggt tctccgagat ccgaaatcag cagagaatta gctccgcgtc
     4921 cggagaagtc ccacgtgtgc gcgccttcca cctcgtcgca aacccggatg cagcggctgc
     4981 aaagaacgca ccggttatga tccaggccga agcgttcgtg cgacgtgtcc accgaaaggg
     5041 cgggaaaccg gtaacgaagc cggacatggt cgatgccgag cgaagcggcc aggtcctgca
     5101 attcgcacgc gccattcacg acacacaccg cgcagacatg gtttctttcc gcgagcaaca
     5161 gctcgacgat catccgccgg tgggcaacca gccgctcggt atcggtgcgt atgaccattc
     5221 cctcttccac cggagtcatg caggcggcca gcagcttgcg ctggccttct acctccacca
     5281 tgcacagccg gcaggcgccg acatcggaca cgcctccgac gtggcacagc cgcgggatgt
     5341 ggattccgtt gtcccacgcc acgctgaaaa tggtctggcc ggcaacaccc gttacggcct
     5401 taccgtcgat ttcgagagtt ctgacactca tgcctgaacc ttcccatttc cgctca
//
LOCUS       DASTMK010000201         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_10832, whole genome shotgun sequence.
ACCESSION   DASTMK010000201 DASTMK010000000
VERSION     DASTMK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10832"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFQ79_08760"
     CDS             complement(<1..456)
                     /locus_tag="VFQ79_08760"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139787.1"
                     /translation="MPLAKAKILVEATGEQFEVMFNPEEYTINKDNNFASQNVPGLSG
                     PLLQFVHGNLRTLEMELLFDTYEKHTDVRDEAKKVLDLLNINRDLHAPPVVRVSWGSL
                     DFRCVLARASQKFILFFPDGRPARVRINVTFNEFIDADREAKEINRQTAD"
     gene            complement(460..1155)
                     /locus_tag="VFQ79_08765"
     CDS             complement(460..1155)
                     /locus_tag="VFQ79_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139788.1"
                     /translation="MKRSIASTNTRRYKGNRWLAWARAIRERRGLPQLRRAPLAMRFV
                     SRLRTLVLVHHKTMREVSTTSISYHDAATVSLFPRIDLHFTNLYSTVLAQRFQTAGAV
                     TVEPGVLRTGFSGGGHRTANDFAIPLPHYLAGLHTHVSVQRVIERTRRVEHRMPPASR
                     ILPQVAMKPVEAAAANGRGASIPVTGRTSDDATRFRPSAAPAQINVEQITDHVLRQLD
                     HRLLAWRERTGRS"
     gene            complement(1285..1650)
                     /locus_tag="VFQ79_08770"
     CDS             complement(1285..1650)
                     /locus_tag="VFQ79_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139789.1"
                     /translation="MFQTEYEFTLPFGYADNEGNLHRSGVMRLATAADEILPLRDPRV
                     QANQAYLIVILLSRVITRLGSLAQVNPKVIEGLYAADLAYLQEFYNRINRNGAATVEA
                     MCPKCEHEFAVELSAPGGF"
     gene            complement(1654..2097)
                     /locus_tag="VFQ79_08775"
     CDS             complement(1654..2097)
                     /locus_tag="VFQ79_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU0139790.1"
                     /translation="MPQTGQRVDPYGSFDFLVEIDGIARAAFSEASGFDSSIDVVEYR
                     EGGDIPTMRKLPAKTKYSNITLKWGSTNDRELYDWHMQWIKGDPAATRRNGSIVMLDR
                     GGQEVARWNFVNAWPMKWTGPSFNAKNNDVAIETLELAHEGIERA"
     gene            complement(2116..4125)
                     /locus_tag="VFQ79_08780"
     CDS             complement(2116..4125)
                     /locus_tag="VFQ79_08780"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEU0139791.1"
                     /translation="MPILDVPGVYIDEVTSPGVIAGVGTSTAAFIGPALAGPINQARR
                     ISSFDEFLQLYGQPQADGTINPYIVTPRVFYLAHGVRGFYENGGKQAYIVRVGTARAA
                     AWDIVNGNTPTAETAFRLQARQEGLSGNNITIQVQAASATSATGVSVATATANVTAVN
                     GINLTVNDASNFRVGDVVTESGGTENFGNRATITQIQGNNITLSNTIAGLAASENIRI
                     ASILPEQTTFRLAATTGIFPGTVAQISGDDADNPGTTVNEYVLVQTVDAGGFVTVAPT
                     PARATRYNLAAATAPVLISEEFRLIVTPPSGPAETFDNLSLSPSHPNYVFTAVSSGWV
                     DVLPPSTPPTAGIPENLIPAVAGDVAAAVTGVNDDPGSLTSAGFQTGINALRDIDDVN
                     ILAIPDAAAHPEALTIQLALINHCLLKRDRFAILDSRLGAPPSGPGSVEEHVLQVRSD
                     RGFAALYYPWLTVRDPMSTGPIPRTMLIPPSGAMAGIYARVDGERGVHKAPANTDVRG
                     VLGLERVLSDGQHGPLNLLGVDVLRIFPGQAQVTVWGARTTIDPNITDWLYVNVRRLM
                     LYIEESIEEGIRWAVFEPNGLPLWQKLKRNITEFLTRVWRDGALFGATAEKAFYVRID
                     EALNPPSTRALGRLYIEIGVAPVRPAEFIIVRIGLWDGGASISES"
BASE COUNT          747 a         1265 c         1293 g          844 t
ORIGIN      
        1 gtcggcggtc tgccggttga tctccttggc ttcgcgatcg gcatcgatga attcgttgaa
       61 ggtgacgttg atacgaactc gcgcgggcct tccatccggg aaaaacagga taaacttctg
      121 gctggcgcgc gccagtacgc agcggaagtc gagcgaaccc caggaaacgc gaaccaccgg
      181 cggcgcgtga agatcgcgat tgatgttcaa caggtcaagc actttcttcg cttcgtcccg
      241 tacatccgtg tgcttctcgt aggtgtcgaa cagcagctcc atttcgagcg tgcgcaggtt
      301 tccgtgcaca aactgaagca gagggccgct cagcccggga acattctgcg aagcgaaatt
      361 attgtccttg ttgatggtgt actcttcggg gttgaacatc acctcgaact gttcgccggt
      421 cgcttccacc agaattttcg cttttgccag gggcatgggc taggacctcc ccgttcgctc
      481 gcgccaggcg aggaggcgat gatcgagctg gcgcagaacg tggtctgtga tctgctccac
      541 gttgatttgc gccggagcgg ccgacgggcg aaagcgggtg gcgtcgtccg aagtgcggcc
      601 cgtcacaggt atcgaggcac cgcgtccgtt ggcagcggcc gcctcgaccg gtttcatcgc
      661 aacctgtggg aggatgcgcg aagcgggcgg catacggtgt tcgacgcgtc gcgtccgctc
      721 gatcacccgt tgcaccgata catgtgtgtg aagccccgca agatagtgcg gcagcggtat
      781 agcgaaatcg ttcgccgttc gatggccgcc gccggaaaat ccggttcgta acacacccgg
      841 ttcgaccgtg accgcaccgg ccgtttgaaa tcgctgcgcg agtacggtgc tgtataaatt
      901 cgtgaagtgc agatcgatgc gcggaaacag ggaaacggta gctgcatcgt gataggagat
      961 cgatgtggtg gaaacttcgc gcatggtctt gtgatgtacc agcacgagcg ttctcaaacg
     1021 cgacacgaat cgcatggcca gcggcgcgcg gcgaagctgc ggcaaccctc gccgttcgcg
     1081 tatcgcgcgc gcccaggcga gccagcggtt gcccttatac cggcgagtgt tcgtgctggc
     1141 gattgatctt ttcattgatc gcggaaatct cccgtgccca cctgcgccgg tccgcgtgct
     1201 cgagcgcgag caccgcctcc ggctgccaat gaaagtgata cgcgacgaag gctacctcct
     1261 cgtagagagc ttcgaggggg tagcctaaaa cccccccggc gcgcttagct ccaccgcaaa
     1321 ttcgtgctcg catttcgggc acatcgcctc tacggtggcg gcgccgttcc tgttgatccg
     1381 gttataaaac tcctgcaaat acgccaagtc cgcggcgtac agcccttcaa tcactttcgg
     1441 gttcacctgc gccagtgacc ccagccgcgt gatgacgcgc gagagcagga ttacgatcag
     1501 ataagcctgg ttcgcctgca cgcgcggatc gcgcagcggg agaatctcgt cggcggctgt
     1561 cgccagccgc atcacgccgc tccggtggag attgccctcg ttgtcggcgt atccgaacgg
     1621 cagcgtgaat tcgtattcgg tctggaacat gggttatgcc ctctcgatgc cttcgtgggc
     1681 cagttccagc gtctcgatgg cgacgtcgtt gttcttggcg ttgaagctgg gcccggtcca
     1741 cttcatgggc caggcgttga cgaagttcca gcgcgctact tcctggccgc cgcgatcgag
     1801 catgacaatg gacccgtttc tgcgcgttgc cgccggatcg cccttgatcc attgcatgtg
     1861 ccagtcgtac aattcgcggt cgttggtgga accccacttg agcgtgatgt tggagtactt
     1921 ggttttcgcc gggagcttcc gcatggtggg aatgtctcct ccctcgcggt actccaccac
     1981 gtcaatcgag gaatcgaagc cactagcttc ggagaaggcg gcccgggcga tgccgtcgat
     2041 ctccaccaga aaatcaaaac tgccgtaagg atctactctt tgtccggttt gaggcatagt
     2101 tcactcctgg aaactctagc tttcggaaat ggaagcaccg ccgtcccaca ggccgatgcg
     2161 aacaatgatg aattcggccg ggcgcaccgg agcgacgccg atctcgatgt acagccgtcc
     2221 cagagcgcgc gtcgacggcg gattcagagc ttcgtcgatg cgcacgtaga acgctttctc
     2281 cgctgtcgcg ccgaacaatg cgccgtcgcg ccaaacccgc gtcagaaact cggtgatgtt
     2341 gcgtttcagc ttctgccaga gcggtaggcc gttgggctcg aacaccgccc accgtatgcc
     2401 ctcttcgatg gactcttcta tgtacagcat caggcggcgc acattcacat acagccagtc
     2461 ggtgatgttg ggatcgatgg tggttcgtgc gccccaaacc gttacctgcg cctgccccgg
     2521 gaagatgcgg agcacatcca cgcccagcag attgagcggc ccgtgctggc cgtcgctcaa
     2581 cacgcgctcc aggcccagca cgccgcgcac atccgtattc gcgggcgcct tgtgcacgcc
     2641 gcgttctcca tccacgcgcg catagatgcc ggccattgct cccgagggtg gaatcagcat
     2701 ggtgcgggga atgggcccgg tgctcatggg gtcgcgcacg gtgagccacg gataatacag
     2761 cgcggcgaag ccgcggtcgg accggacctg gagcacatgc tcttccacgc ttcccggtcc
     2821 gctgggaggc gcgcccagcc gcgagtcgag gatcgcaaaa cggtcgcgct tcaacaggca
     2881 gtgattgatg agagcgagtt gaatggtgag cgcttcgggg tgggcggctg catccggaat
     2941 cgcaagaatg ttcacgtcgt cgatgtcgcg caacgcgttg atgcccgtct ggaatcccgc
     3001 tgacgtcagg cttcccggat cgtcgttcac gcctgtcacc gccgcggcca catctcccgc
     3061 cacggcagga atgaggtttt ccggaatgcc ggcggtgggc ggcgtggagg gtggcagcac
     3121 gtccacccaa ccggagctca ccgcggtgaa gacgtagttc ggatgcgacg ggctcagcga
     3181 gagattatcg aacgtttccg ctggacccga aggcggcgtc acgatcaggc gaaactcttc
     3241 agagatcaga accggcgcgg tggccgctgc caggttatat ctggttgcac gcgcgggcgt
     3301 gggcgccacg gtcacgaagc cgcccgcatc cacggtttgc accagcacgt actcgtttac
     3361 cgttgttccg ggattgtcgg catcgtcccc gctgatctgc gccacggtcc ctggaaagat
     3421 accggtagtg gccgcaaggc ggaacgtggt ctgctccgga aggatgctgg cgatccggat
     3481 gttttcactc gcggcaagcc ctgcgatggt gttgctcagc gtaatgttgt tgccctggat
     3541 ctgcgtgatg gtggcgcgat tcccgaagtt ttccgtgccg ccggattccg ttaccacatc
     3601 ccccacgcga aaattgcttg cgtcattcac ggtgaggttg atgccgttca ccgccgtcac
     3661 attcgccgtg gccgtggcaa cggaaacacc tgtcgcactg gtggcgctgg cggcctgcac
     3721 ctgtatggtg atgttgttcc cactcagccc ttcctgcctg gcctgaaggc gaaaagcggt
     3781 ctcggcggtg ggcgtatttc cgttgacgat gtcccaggcg gctgcccgag ccgtgcccac
     3841 tcgcacgatg taagcctgct tgccgccgtt ctcatagaac ccgcggacgc cgtgcgccag
     3901 gtagaagacg cgcggcgtca cgatataagg gttgatggtg ccatccgcct gcggctgccc
     3961 atagagttgc agaaactcgt cgaagctgct gatgcgccgc gcctggttga tggggccggc
     4021 aagcgcgggc ccgatgaaag cggccgtgct ggtgccgact ccggcgatca cccccgggct
     4081 ggtcacttcg tcgatatata cgcctggtac atcaagaatc ggcataacct gttcgctcct
     4141 gttctaagt
//
LOCUS       DASTMK010000202        13463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_106098, whole genome shotgun sequence.
ACCESSION   DASTMK010000202 DASTMK010000000
VERSION     DASTMK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106098"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1724)
                     /locus_tag="VFQ79_08785"
     CDS             complement(213..1724)
                     /locus_tag="VFQ79_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU0139792.1"
                     /translation="MAANTQVRLSLALSVCACCLVAPLSGAPADPEDPLFRAVDLNVG
                     ENREVRFPDGTAASVRLIARDEVRDNVRQAVRSARVRVEVNGRETMLNCGNYRLPLTV
                     GGVQIDCPVTKGYLDNARTNPWGLDKDARLRLWPADSPLLRPGTYVYPVQQRWFASDT
                     QMSNEPSFVDGSEEPARKKVYYHYGLDIGGVEGLTPVISATGGLVVVRGNDVLPAYKD
                     SPYIEINYDGVIVLDERGWFHWYFHLARIDPGVTLGERIRMGQNIGVIGKEGHAGCWS
                     HLHYEIRSLQPSGKPGIQEGYAFLWEAYQRQYSPDLVAVARPHSLAWAGEEVVLDGGS
                     SWSRSGKIELYEWTFTDGSRAEGSQVKRVYAKPGTYSEILKITDAAGHVAYDFAVVQI
                     VGKPENERADRLPPTIHAAYGPALDVRPGQSLTFTVRTCRTTHGQETWRFGDGSAPVV
                     VKSDGCAEPASPKGYAFTRHAYKNPGDYIVRVERANERGERAIGHLHVRVRGQ"
     gene            complement(1763..3589)
                     /locus_tag="VFQ79_08790"
     CDS             complement(1763..3589)
                     /locus_tag="VFQ79_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEU0139793.1"
                     /translation="MTDMQAFPYTEAAALALLHFLWQGTALAVAAAAALRILRARSPE
                     FRYWTACLFLLSMLLLPAITCFLLLRETPAFVPSGSLEPFETQAFSGLALEMKPDQPW
                     LSLVLVLWLAGVSVFSLRAIGGWLLAAWRFSTHRSIVSADLRAKTAALSHRIGIRRAV
                     RVFQSARVGVPCVFGVIRPVLMLPASMLSGIPPSHIEAILAHELAHIRRFDHIVNCLQ
                     AAVEALLFYHPAVWWLSARIRQEREMCCDAIAVAVCGDRVLYSRALLGLEERRQAFTI
                     AATAGNLKSRIERLFQPGAERKFHPAPAAFAMGLLLILLTTPVAVIAMLAPGGQGSSP
                     PHPAEALRDPDPSTPVNEAAEEHARRIRHANQHFSGSRPGSETARGRVYIENGPPHEM
                     EVTIYREIWRYPDGHTFEFAGLDHELQREYTSRLQHPPPVLERILLDRLSEPARSQLR
                     KRLAPFQRQPFTEGSRRSLTIAAGEIDPSMTYSWRSDPATGNVTLELSHEEPAFDGNP
                     HPGFAATGERRIRIGSRAMRSKLAYSPRPDNPAGETGTVRLAVLIGTDGRIRDLYVLR
                     GSPKPAAVAEKAVRTWQYQPTFLDGIPVEVATIVDVPFGALR"
     gene            complement(3586..3984)
                     /locus_tag="VFQ79_08795"
     CDS             complement(3586..3984)
                     /locus_tag="VFQ79_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0139794.1"
                     /translation="MRKKQAPLPKPTAAELEILAVLWRTGASTVREVHAGLANSQAVG
                     YTTTLKLMQIMAGKGLLIRDERDRAHVYHPAVKQEQTQRQIVHDLLDRVFGGSASQLV
                     LRALSGRSATKQEVEEIRRMLDDIEHRSKS"
     gene            complement(4116..7211)
                     /locus_tag="VFQ79_08800"
     CDS             complement(4116..7211)
                     /locus_tag="VFQ79_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0139795.1"
                     /translation="MRLSEICVNRPVFAFMLIMFLVVMGVFSFLQLGVDLFPKSDAAT
                     VYVRMRLPGASPEEMTSQVVLPLEESIAAISGIDEMRAIVSEGNANIVVTFVLERDIG
                     EAVEDVREKVSAAVRNLPPNILPPVVAKSDPDSDPVITLAVSGDRPVRELTEIADKQI
                     RRAIETVDGVGGVDINGGQLRQINVYLDINKLSGYQFTAQDVENAIRTENIEAPGGRI
                     VRGPTEVGVRTMGRVEHVADFNNIIVKNVGGAPIRIRDIGHVEDGMAEKRTFGFYQGT
                     PAVVLEVRRQVGMNTVKVVDGIQHRLEEIKDQLPSGVSINVVKEQATYIKNSVAALEE
                     HLLLGSLLASFIVWLFIRDWRTVLISSIAIPTSIIATFTFMRALDFTLNSMTLLGLTL
                     AVGIVIDDAIIVLENIYRHIEEHALPPMEAAVKATREIALAVMATTLSLVIIFVPIAF
                     MTGYARRYMNQFGWTMAISILVSMLVAFTLTPSLSSRLLKRRDPAKRGGHEGGHGHKH
                     GFFDRKYVRLLDWSLTHRWVVVGVAILTFASTFVLARFIGRDWMPQDDQSELSVHLEL
                     PEGSSIEATEKLTLEIAKRIQQIPGIRAVVPNTSSFLNRVTMSFITVLLEPQGERDDI
                     QVMGQQVRAVLRDYPYARPRVSFPNVLGGRDTFSPIRAMLLGPDMDKLVEIAKEVNVK
                     LTTQPELADIKVNLNLNNPELQVKIDRQLASDLGVRVSDVANAVRLLMSGEDEISTYK
                     EESEQYPVTMRLLPGQRDDPAVLSRLLVPSSRLGLIRLDSVATLERGLGPSRIDRFAR
                     QFAIGIYGNIAPGHTLGEGAAVTQRIINEVGLPPGYRLTFSGQVKILEETTLNMILAF
                     ALASIFMYMVLAAQFESLAHPFIILLTLPLSIPFALISLIGTGRSLNLFSALGVLLLL
                     GIVKKNGILQIDYMNRLRDQGRPLHEAILEANRIRLRPILMTTFSIIAGLIPTAIGIG
                     AGASQRSAIAVTIIGGQTLCLLLTLVVVPVGYAMVEDAKAWIARKRHVEQAATAPAAG
                     D"
     gene            complement(7332..9989)
                     /locus_tag="VFQ79_08805"
     CDS             complement(7332..9989)
                     /locus_tag="VFQ79_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139796.1"
                     /translation="MIQPFRTAWLRLKALMYRRRLDRDLEEELRLHTVMRAQKFTAEG
                     MPPKAAQAAAERQFGNRTRVRETCRELWTFSTIEALWQDVRYGLRTLAKAPVFSAVAI
                     ISLALGIGANTALFSLMDVMLMRELPVKNPQQLVEFIRASPSAMMTNLPYPVFSYFQR
                     DQEVLEDVFTISPSQAVVNAGAGAERAQAHLVSGSFFPALGVNALMGRSMGPSDDRHS
                     VAVLSHGFWSRRFGADPSILGRTVRVNGEPFTVVGVMPPAFFGVDRSEIPDLWLPIPA
                     GVRPQAEVWVLGHLKPGVSVSQAQTVLEPLFGQALESLTGEIERWPERDRKAFLSQKL
                     LIRSATSGTVDLEWTYWKGKATLKVLMGLTVLVLLIACVNLANLLMARSANRTREIGI
                     RLGIGAGRFRLVRQLLTENLLLSLAGGAIGLVVAAWGHRLLLGFLVRDPQSVALDFRL
                     DYRVLCFGLALSVTTCVLFGLMPALRATHPNLIGAIRGDANERGPARIPLARTLLPLQ
                     VALSMVLLMGAGLFARSLRNLGMADLGLVRSNLLMTEVRAPGKAPQEHQQFWTRFGEQ
                     VSAVPGVRSVALAGSVAFGSGGWNQTVWIDRAGQPAQDAKISHNIVSPGFFATVGIPV
                     LIGRGFGEHDRKGSPLVAVVNQTFARRYLPNENPIGKRFGDRGRDSSGRYEIVGVVGD
                     AKYGAVREPMRPMAFYPLLQQEPRNLMAVHVRTAAGPDALVPSIRRKILAIDGESLVS
                     EIRTLPQVVRSQLRQDRMFATLAAFFAALALALAAIGIYGIVAYRVTRRMPEIGVRIA
                     MGAQRKDVLWLVMKETLTLFAIGGLLGAACALVASRLIRSLLFALEPSDPATLASAGV
                     ILFTVGVFACLLPARRAASLDPLVALRIE"
     gene            complement(10001..10339)
                     /locus_tag="VFQ79_08810"
     CDS             complement(10001..10339)
                     /locus_tag="VFQ79_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139797.1"
                     /translation="MGAQDKPGDLVQGTLDMLVLKTLTRGAAHGYAIAEFIQATSNEV
                     LRVEEGALYPALHRLEIRGLLASEWGVSENNRRAKYYRLTAAGRKQLAAEADRWSRMT
                     AAISMVMNSA"
     gene            10458..12254
                     /locus_tag="VFQ79_08815"
     CDS             10458..12254
                     /locus_tag="VFQ79_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139798.1"
                     /translation="MGRLFGLFLLVSVSYGQYANLRTTDDGAILYFSSSLAMRGTSQF
                     DHPKLFAIDSGGLRLEAQRELQGPAVGTPYGLTNYYRLNSVEVSGDGMVRAIVSGRVC
                     SYGGSSCFGIPPKIQTEITGIPGKAAVTLTGQARLSRNGRYAVVCCDGSMVSQARLVD
                     LYTGEEVVLERDSYLGFYTSQPAGRMVTPDGRAVFRGGGGIVLGGLAGSEAIITSESP
                     GQIFVDDSGLFAAYDTHTPLVTITRNRLWRVDLGTGKETRLAEGLNLRLAGMSNDGLL
                     VLFLMDIGGTPQLFVSNERGEIRQLTTDTAGIPETVLSGDGTVVYAGTYSGRLLRIDV
                     ETGLTRELVGRAVVLDDPSLSSSSASIAGSAYCVSGFGLADTASFTFPPLPRKWNGLE
                     ILLDGEPVPLQSVTPTQACFQIPWDTGVGDHKFSVSTEISSAFESVVEGTLRLYHEVM
                     PYFVFSGDTGSQTFRQFAAAGHEDFRGRVTPHDPARPGEIVHFYMTGLGPVIPAIGNG
                     IAGPANPPSRTQAQLSCRMVGSPAEPTPAEVLFSGLAPGFVGYYQVSIRLPSQFPFSD
                     FGQGEVNATIACSVDGIRSAAVAAIPVKPDAI"
     gene            complement(12247..12681)
                     /locus_tag="VFQ79_08820"
     CDS             complement(12247..12681)
                     /locus_tag="VFQ79_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU0139799.1"
                     /translation="MADDKQQRTQQLKEKYGSALRVIEQQGVRLENVHVENNKLLIRG
                     VAPSEDAKNKVWDQIKLVDPSYSDLTCDLRVEQQAGQAAAAGQTQAAAQTYTVQAGDS
                     LSKISKQFYGDANAYMRIFEANRDKLSDPDKIKPGQQLTIPK"
     gene            13039..>13463
                     /locus_tag="VFQ79_08825"
     CDS             13039..>13463
                     /locus_tag="VFQ79_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139800.1"
                     /translation="MSTQPIHGNPAPDLVQIRSSIEDIIHQRLAEERARLEREAGLAN
                     HGVHHFKRPVERTFTKSERGRTTLLFGGLTWKHEKLVHGALEGLGYLAETIPTPNVAA
                     FQLGKEYGNNGQCNPTYFTVGNLVQYLQQLEEQGVPKQE"
BASE COUNT         2713 a         4034 c         3986 g         2730 t
ORIGIN      
        1 cgccgctgct gttttcattg gcacgctcat tgtctcctcc attgaaatcg gttcagtcag
       61 tacaacagac gcagttcgag ggccaaaaga tttggaaaat tcgtaaagat tcttaaactc
      121 gcaagtgtag gaagaacatc agcggcggcg tacgatcagc ggaccgggtg acggtaaatt
      181 gtcattggct actgacaaaa tttcccacgc ggctattggc cgcgcacccg gacgtgcagg
      241 tgtccgatgg ccctttctcc ccgctcattc gcccgctcta cccggacgat atagtcgccg
      301 gggttcttgt aggcatgccg ggtaaaggcg tatcccttgg gggatgcggg ttccgcacag
      361 ccatcggact tcaccacgac gggagcgctg ccgtcgccga atctccacgt ttcctggcca
      421 tgcgtggtgc ggcaggtccg gacggtgaac gtcaggctct ggcccggacg cacatccaat
      481 gctggtccat acgccgcatg gatcgtggga ggcagccggt ctgcccgttc attctccggt
      541 ttgcccacaa tctgcacaac ggcaaaatcg taggcgacgt gtcccgccgc atcggtgatt
      601 ttcaggattt cgctataggt gcctggtttt gcgtaaaccc gctttacctg cgagccttct
      661 gcgcggctgc catccgtaaa ggtccactcg tagagctcga tctttccgga ccggctccag
      721 gaagaaccgc cgtccagaac gacttcttcc ccagcccagg caagtgagtg cggccgggcg
      781 acggcaacga gatcgggcga atactgcctt tgataagcct cccaaagaaa ggcgtaccct
      841 tcctgaatgc caggttttcc agagggctgg agggagcgga tttcataatg cagatgagac
      901 cagcaaccgg catggccctc cttgccgatg acgccaatat tctgccccat cctgattcgc
      961 tcgccgagag taaccccagg atcgatccgc gcgagatgaa agtaccagtg gaaccatccc
     1021 cgctcgtcaa gcacgataac gccgtcgtag ttgatttcga tgtaagggct gtctttatag
     1081 gcgggcagta cgtcattgcc tcggactacc accaggccgc cggtagccga gatcacggga
     1141 gtgagtcctt cgacgccgcc gatgtcgagc ccgtaatggt agtagacctt ttttctggcc
     1201 ggctcctccg agccgtcgac aaaggacggc tcattcgaca tctgcgtgtc gctggcaaac
     1261 cagcgttgtt gcacagggta gacatatgtg cccggacgca gcagcggtga gtccgcgggc
     1321 caaagccgga gccgtgcgtc tttgtcgagt ccccaggggt ttgtacgggc gttgtcgagg
     1381 tatcctttcg tgacgggaca gtcgatctgc acgccgccca cggtgagcgg caaccggtag
     1441 ttgccgcagt taagcattgt ttcgcggccg ttcacctcta cgcggacgcg cgccgagcgt
     1501 accgcctgcc ggacgttatc gcggacctca tcgcgggcga tcagtctgac gctggccgcc
     1561 gtcccgtcag gaaaacgcac ctcccggttt tctccaacgt tgaggtcgac ggcccggaaa
     1621 aggggatctt cagggtctgc cggagcaccg gataacggcg ccacgagaca gcacgcacaa
     1681 accgagagcg cgagggacaa acggacctgg gtattggcag ccacgcatcc agtgtaggtc
     1741 gaagccagga cgcgaggaac ccttagcgca gagctccgaa ggggacgtct acgatggttg
     1801 cgacttcgac cggtataccg tccaggaacg tgggttggta ttgccatgtc cggaccgctt
     1861 tttcagccac cgcggccggc ttcggcgaac cgcgcagtac atagagatcg cgtatccgtc
     1921 catcagttcc aatcagcacg gcgagcctga cggtaccggt ctcaccggcc gggttgtccg
     1981 gccggggtga atatgccagt ttggatcgca tggccctgct tccgatgcgg atccgccgct
     2041 ccccagtggc agcgaaaccc ggatgggggt tcccgtcgaa ggctggttcc tcatgcgata
     2101 gttcgagtgt aacgttaccg gttgcgggat cgcttcgcca tgaataggtc atcgaggggt
     2161 cgatctctcc cgccgcgatg gtgagcgagc gcctggaacc ttcagtgaac ggctgtcgtt
     2221 gaaacggtgc aaggcgtttt ctgagctgtg agcgggccgg ctcagacaga cggtccaata
     2281 gaatgcgctc cagcaccggc ggcggatgct gtaaacgcga tgtatattcc ctctgtaact
     2341 catgatcgag gcctgcgaat tcgaatgtgt gtccgtctgg ataccgccag atttcacggt
     2401 aaatcgtcac ttccatctca tgcggcggac cgttctcaat gtaaacgcgc cctcgtgctg
     2461 tttcgctgcc cgggcgtgag ccggaaaaat gctgattcgc atgccggatc cgccgcgcgt
     2521 gttcctctgc cgcttcgttc accggcgtgg agggatccgg gtcccggagc gcttccgcgg
     2581 ggtggggagg cgatgatccc tggccgcccg gtgcgagcat ggcgataacc gccaccggag
     2641 tagtgagaag gatcagcagt aatcccatcg cgaaggctgc cggtgccggg tgaaacttac
     2701 gttcagcgcc gggttggaac aatcgttcga tccgcgattt cagattgccc gcggttgccg
     2761 ctatagtgaa cgcctgacgc cgttcttcga gccccagcag cgcgcgcgag taaagcacgc
     2821 gatctccgca aacggcgacg gcaatcgcat cgcagcacat ttcccgttcc tgccggatgc
     2881 gcgcggaaag ccaccacact gccgggtgat aaaacagcaa tgcctcaacc gccgcctgca
     2941 aacagttaac gatgtggtcg aagcgccgga tgtgggccag ttcgtgcgcg aggatggcct
     3001 cgatatggct gggaggaatg ccgctcaaca tgctggccgg aagcatcagg acaggacgaa
     3061 tgactccgaa gacgcaggga acgccgactc gcgcggattg gaagacgcgc acggcccggc
     3121 ggattccgat gcggtgggac agcgcggcgg ttttggcgcg cagatccgcg gaaacgatgc
     3181 tcctgtgggt ggagaaccgc caggccgcaa gcagccaacc gccaatcgcc cgcaggctga
     3241 aaaccgatac gcctgccagc cacagcacca ggaccaagga aagccacggc tggtccggtt
     3301 tcatctccag cgccaggccg gaaaacgcct gcgtctcgaa gggctccagg ctgccggatg
     3361 gcacaaaagc aggtgtttcc ctcaataaaa gaaagcaggt aatcgccggc aacaacagca
     3421 tggacagcag aaacaggcat gctgtccaat agcgaaattc agggcttcgc gccctcagaa
     3481 tccggagggc agccgcggcg gccacggcca gggccgtgcc ctgccataag aaatgcagca
     3541 gggcgagggc ggccgcttcc gtgtacggga acgcctgcat atccgtcatg acttgctcct
     3601 gtgctcgatg tcgtccagca tccgccggat ctcttccacc tcttgtttcg ttgcgctgcg
     3661 cccggaaagc gcgcgcagga cgagttgcga agccgatccg ccaaataccc ggtcgaggag
     3721 atcgtgaacg atctgccgct gcgtctgttc ctgtttcacg gcggggtggt agacatgcgc
     3781 ccggtcgcgc tcatcccgga tgagcaggcc tttcccggcc atgatctgca tcagtttcag
     3841 ggtggtggta tatccaaccg cctggctgtt tgcgagtccg gcatggacct cccgtacggt
     3901 ggacgcgccg gttctccaga ggactgccag gatctccagt tcagccgccg tcggctttgg
     3961 caggggcgcc tgctttttcc tcatgaagcc agcatatatg aaggtcttcg tatatgtcaa
     4021 cgaaatcttt cgtactattt ttaaacacga cgtcctaaat gtttgcgcga cgatttgctt
     4081 ttctgtttag caggcttgag gcggggaatt gccgctcagt cgccggcggc tggcgcggtt
     4141 gccgcttgtt cgacgtggcg ctttctcgcg atccaggcct tggcgtcttc caccatcgcg
     4201 tagcctaccg gtaccactac gagtgtcagc agcaggcaga gcgtctgccc gccgatgatc
     4261 gtgacggcga tggcagagcg ctgcgacgcg cccgcgccga tgccgatggc tgttgggatc
     4321 agacccgcaa taatcgaaaa tgttgtcatg aggatgggcc gcagccggat gcgattggct
     4381 tcgagaatcg cttcgtgcaa gggacgtccc tggtcgcgaa gccggttcat gtagtcgatc
     4441 tgcagaatac cgtttttctt aacgatacca agcaacagca gcacaccgag cgcgctgaag
     4501 aggttcaggc ttctaccggt gccgatcagc gaaatgagcg cgaatggaat ggacagaggc
     4561 agcgtcagca gaatgatgaa ggggtgagcc aggctctcaa actgcgctgc cagcaccatg
     4621 tacatgaaga tcgacgcgag ggcgaaggcg agaatcatgt tcagcgtcgt ctcttccaga
     4681 attttcacct gcccggagaa agtcaggcgg tatcctggcg gcaaaccaac ctcgttgatg
     4741 atcctctgtg tgaccgcggc gccttcaccg agcgtgtgcc cgggggcgat attaccgtag
     4801 atgccgatcg caaactggcg ggcaaaccgg tcgatacggc tggggccgag cccacgctcg
     4861 agcgttgcaa ccgagtccag gcggatcagc cccaaacgcg acgagggaac gagcaggcgg
     4921 cttaacaccg cagggtcgtc acgctgcccc ggcagaaggc gcatcgtaac agggtactgt
     4981 tcggactcct ccttgtaagt ggagatttcg tcttcgccgg acatcaggag ccttacggca
     5041 ttggcgacat cggagacgcg cactcccaga tcggaagcca attgtctgtc gattttcacc
     5101 tgcagctcgg ggttgttcaa gttgaggtta accttgatat cggccagttc aggctgtgta
     5161 gtcagcttga cgttgacttc tttggcgatc tccaccagtt tgtccatgtc gggaccgagc
     5221 agcatggcgc ggatcggcga gaacgtatcg cgccctccca gcacgttcgg gaaggacacc
     5281 cttggcctgg cataggggta atctctcaga accgctctca cctgctgtcc catgacctga
     5341 atgtcgtccc gttcgccctg cggttccagc agcaccgtga tgaaagacat ggtcacgcgg
     5401 ttcagaaatg acgatgtgtt cggcacaacc gcgcgaatgc cggggatctg ttgaatccgc
     5461 ttggcaattt cgagcgtcag tttctccgtc gcctcaatcg acgatccctc cggcagttcg
     5521 agatgaaccg acagctcact ctggtcgtcc tgcggcatcc agtcgcggcc gataaagcgc
     5581 gccaggacaa aggtggaggc aaaggtgaga atcgcgacac ccaccaccac ccaccggtgt
     5641 gtcaaagacc agtcgagcaa acgcacatac ttcctgtcga aaaacccgtg cttatgaccg
     5701 tgcccgcctt cgtggccacc ccttttcgcc ggatccctgc gcttcagcaa ccgcgaactc
     5761 agcgatggcg taagtgtgaa agccaccagc atcgatacca gaatcgaaat ggccattgtc
     5821 catccgaact ggttcatgta acggcgtgcg taacccgtca tgaaagcgat ggggacgaag
     5881 ataatgacca gcgacaacgt tgtcgccatc accgccagcg caatctccct ggtggccttg
     5941 acggccgctt ccatcggtgg aagcgcgtgc tcctcgatat gccggtaaat attttcgagc
     6001 acaataatcg cgtcgtcgat gacaatgcct accgccagcg tcagccccag caaagtcatg
     6061 gagttcaacg tgaaatccag ggcgcgcata aacgtgaagg tcgcaatgat ggacgtggga
     6121 attgcgatgg aactgatcag aaccgtccgc cagtcgcgaa taaacagcca gacgatgaag
     6181 gaggcaagca ggctgccgag cagaaggtgt tcttccagtg cggctaccga atttttaata
     6241 taggtggcct gttccttcac tacgttgatg gacacgccgc tcggaagctg gtctttgatt
     6301 tcttccagcc ggtgctgaat tccgtccaca accttcacgg tgttcatgcc gacttgccgg
     6361 cggacctcca ggacgacggc gggcgtgccc tggtagaagc caaaggtgcg cttttccgcc
     6421 atcccgtctt ccacatggcc tatgtcccgg atgcgaatcg gcgcaccgcc gacgtttttg
     6481 acgatgatgt tgttaaagtc ggcgacgtgc tccacgcgcc ccatggtgcg cacacccact
     6541 tcggtgggac cacgaacgat acggccgccg ggtgcttcga tgttctccgt acggattgcg
     6601 ttctctacat cttgagccgt gaactggtaa ccgctcagtt tgttgatgtc gagataaacg
     6661 ttgatctgcc ggagttgtcc tccgttgatg tcgacaccgc ccacgccatc cacggtctcg
     6721 atggcgcgcc ggatctgctt atccgcgatc tccgtcagtt cccgcacggg ccggtcgccg
     6781 ctgacggcga gtgtgatgac agggtccgaa tcgggatcgg acttcgccac caccggagga
     6841 aggatattcg gtggcaggtt gcgaaccgcg gcgctgactt tttcgcgaac gtcctccacc
     6901 gcttccccga tgtcgcgttc cagcacaaag gtgacgacga tgtttgcatt cccttcggag
     6961 acgatggccc gcatttcgtc gatgccgctg atggcggcga tcgattcctc gagcggcagc
     7021 accacctgcg aggtcatctc ctcggggctc gcaccgggca gacgcatcct gacatacact
     7081 gttgcggcgt cgcttttggg gaacaggtcg acaccgagtt gcaggaagct gaagaccccc
     7141 atgacgacca ggaacatgat cagcatgaag gcgaacaccg gccggtttac gcagatctcg
     7201 gataaacgca caaaaagcct ttcgaactag atacaacgat tgtagcgccg cggaggttac
     7261 agggccggca ccgtagaatg ccgccgacgc gcgcggcttc gcttccgagc cgcgagcgcg
     7321 agggagtcgg actattcaat ccgcagggcc accagagggt cgagggatgc agcccgccgt
     7381 gcaggcaaaa ggcaggcgaa aacccccacc gtaaatagga tgacgccggc gctggccagt
     7441 gtcgctggat ctgacggctc aagagcgaat agcaggctcc tgatcaaccg gcttgccacg
     7501 agcgcgcacg ccgcccccag aaggccgcca atcgcgaaaa gcgtcagggt ctccttcatc
     7561 accagccata gcacgtcttt acgctgcgca cccattgcga tacgcacgcc aatctcgggc
     7621 attctgcgcg tcacccgata tgccacgata ccgtagatcc cgatcgccgc cagagctaga
     7681 gccagcgccg cgaaaaaggc agccagcgtg gcgaacatgc gatcctggcg tagttgacta
     7741 cggaccacct gcgggagtgt tctgatttcg gataccagtg attcaccatc gatcgccagg
     7801 attttacgcc ggattgaagg caccagagcg tccggtcctg ccgccgtgcg cacatgcacg
     7861 gccatcaggt tccgcggctc ctgctgaaga agagggtaga aggccatggg ccgcataggt
     7921 tcgcgtacgg caccgtactt ggcgtcgccc acaacgccga ctatttcgta gcggccactc
     7981 gaatctcgcc cgcgatcacc gaaacgcttt ccgatgggat tctcgttggg caagtaccgg
     8041 cgtgcaaatg tctggttgac caccgcaacc agcggcgatc ccttgcggtc gtgctctccg
     8101 aacccgcgac cgatcagtac gggaattccc accgtcgcaa aaaatcccgg actcacgata
     8161 ttgtgcgaga tctttgcatc ctgcgccggt tgccccgccc gatcgatcca gaccgtttgg
     8221 ttccatccac cgctaccaaa ggcgacgctg ccggcgagtg ccacgctgcg cacccccggc
     8281 actgccgaca cctgctcccc gaaccgcgtc cagaattgct gatgctcctg cggtgccttt
     8341 cccggagccc gtacctccgt catcaacagg ttgctgcgga ccaggccgag gtcggccatt
     8401 ccgagattcc gtaagctccg cgcaaacagc ccggcaccca tcagcagcac catggaaagc
     8461 gctacttgga gcggcagcag cgtcctcgcg agcgggatgc gcgctgggcc acgctcgttt
     8521 gcatccccgc ggatggcgcc aatcaggttg ggatgggtgg cacgcaaggc gggcatgagt
     8581 ccgaacagca cacaggtcgt taccgacaat gccaacccga agcacagcac gcgatagtcg
     8641 agccggaaat cgagcgctac cgattgcgga tcgcggacca gaaatcccag cagcaggcga
     8701 tgtccccacg ctgccaccac tagaccgatc gcgccacccg ccagcgacag caggagattt
     8761 tccgtcagta gctgacggac caggcgaaag cgcccggcac cgatcccgag ccggatcccg
     8821 atctcgcgcg tacggttggc cgaccgcgcc attagcaagt tggcgaggtt cacacaggca
     8881 atgagcagca cgagtacggt gagccccatc aggaccttca gcgtagcctt gcccttccag
     8941 tacgtccatt cgaggtccac cgtaccactc gtggcactcc ggatcagtag tttctgcgaa
     9001 aggaaggcct tccggtcgcg ctccggccac cgttcgattt cgccggtaag ggactcgagt
     9061 gcttgaccga agagtggctc cagtacggtc tgggcttgag aaaccgagac acctggtttc
     9121 agatgtccaa gcacccagac ttcggcctgc ggacgcaccc ccgccgggat tggaagccac
     9181 aagtccggaa tctctgagcg atcaacccca aagaaagctg gtggcattac gcccactacg
     9241 gtgaatggtt cgccgttcac acggacggtg cgccccaaga ttgatggatc cgcgccgaag
     9301 cggcggctcc agaagccatg actgagtacc gccacggaat gccggtcgtc cgacggaccc
     9361 attgagcgac ccatcagggc attcactccc agagcgggaa agaacgagcc cgaaaccagg
     9421 tgcgcctgcg cccgctccgc gcctgcgccg gcattcacca ccgcctgtga cggggagatc
     9481 gtaaaaacgt cctccagcac ctcctggtcc cgctgaaaat aggagaacac cgggtagggg
     9541 agattcgtca tcatggcgga cgggctcgca cggataaact ccaccagttg ttgcggattt
     9601 ttcaccggca gttcccgcat cagcataacg tccatcaggc tgaacagtgc cgtgtttgcc
     9661 ccaatgccta gcgcgagcga gatgatcgca acggcactga acactggcgc tttcgcgagt
     9721 gtccgcaggc catagcgaac gtcctgccag agagcttcaa ttgtggagaa agtccatagc
     9781 tcacggcatg tctcgcgcac ccgcgtgcga tttccgaatt gccgttctgc cgctgcctgg
     9841 gcagctttcg gcggcatccc ttccgccgtg aatttttgcg cccgcattac cgtgtggagc
     9901 cgaagttctt cttcgagatc gcgatccagc cggcggcgat acatcaaggc cttcaagcga
     9961 agccatgcag tcctgaacgg ttgtatcacg gtcttctcct ctaggcgctg ttcatgacca
    10021 tcgaaatcgc cgctgtcatt cggctccagc ggtccgcctc cgcggccagt tgcttccgcc
    10081 cggcagcggt gagccgatag tacttggcgc ggcggttgtt ttccgaaaca ccccactccg
    10141 acgccaggag acctcgtatt tccagacgat gcagtgcggg ataaagtgca ccttcttcga
    10201 cacgaagcac ctcattcgag gtcgcctgga tgaactcggc aatggcgtag ccatgcgcgg
    10261 ctcctcgagt gagtgtcttt aggacgagca tgtccagtgt gccttggacg agatctcccg
    10321 gcttgtcctg tgctcccata aaccgtttat ggatatgtta gcccaaccgc gagataaaca
    10381 caataaggat ctgattttct ttaataatct acggtccccg gctggaattt cggacctggt
    10441 ggtattctga gaccgttatg ggtaggctgt tcgggctctt tctgttggtt tcggtttcct
    10501 atggacagta tgcgaacttg cgaacgaccg acgatggggc gattctctac ttctcctcct
    10561 ccctggcaat gcgcggcact tcccaatttg atcatccaaa actgttcgcg atcgactcgg
    10621 gaggtttgcg gctcgaggct cagcgcgagc ttcagggtcc ggcggttggc accccctacg
    10681 ggctaacgaa ctattaccgg ctgaacagcg tcgaggtcag tggcgatgga atggtgcggg
    10741 ccatcgtgag tggacgggtc tgttcttacg ggggcagtag ttgttttggg atcccaccga
    10801 aaatacagac ggaaatcact gggattccag gaaaggcggc tgttacgctg accgggcagg
    10861 cccgactgag ccgcaatggc cgctatgccg tggtttgctg cgacggatcg atggtatctc
    10921 aggctcgact ggttgatttg tacaccggcg aggaagtggt gctggaacgc gactcttacc
    10981 tcggcttcta cacgtcccag cccgccgggc gtatggtaac gccggacggc cgggcggttt
    11041 tccgtggcgg aggcggaatc gttctcgggg gtctggccgg cagtgaagcg atcattactt
    11101 cggaatcgcc ggggcagatc ttcgtcgatg attccgggct ctttgctgct tacgataccc
    11161 atacgccgct agttaccatt acacgaaaca gactgtggcg cgtggatctc gggacgggga
    11221 aggaaacgcg cctggccgag ggattgaatt tgcggctggc gggcatgagc aacgacggtc
    11281 tgctggtgct atttctgatg gacatcggcg gcactcctca gttgttcgtt tccaatgagc
    11341 gaggcgaaat ccggcaattg acaaccgata cggccggaat cccggaaacg gttctgtcgg
    11401 gcgatggcac ggtggtatat gccgggactt atagcggcag gctccttagg atcgacgtcg
    11461 aaaccgggct tacgcgcgaa ctggtaggac gcgcggttgt gctcgacgac ccgagtctca
    11521 gttcatcatc cgcgtcgatc gcagggtccg cgtactgcgt ctcgggtttc gggttggccg
    11581 ataccgcgag cttcaccttc cctccgctgc cgcgcaagtg gaacggtctg gagatccttc
    11641 tggacggcga accggtgcca ttgcagtccg tcacgcctac gcaggcctgt tttcagattc
    11701 cgtgggacac cggtgtagga gaccacaagt tctccgtttc caccgaaatc tcttccgcct
    11761 tcgagagtgt tgttgaaggc acccttcgcc tctatcacga ggtcatgccg tactttgtgt
    11821 tttccggaga cacaggcagt caaacgttcc ggcagttcgc ggccgccgga catgaagatt
    11881 ttcgcggacg ggtcacgccc cacgatcccg cacggcccgg tgagatcgta catttctaca
    11941 tgacgggact cgggccggtg atacccgcca tcgggaacgg aatcgccggc ccggcaaacc
    12001 caccgtcccg cactcaagct cagttgtctt gcaggatggt gggttcgcca gccgagccaa
    12061 ctccggcgga agttctcttc tcgggtttgg ctcccggatt cgtgggttat taccaggtat
    12121 cgatacgtct gccgagccag ttcccttttt ccgactttgg ccagggcgag gtgaatgcaa
    12181 cgattgcctg ctcggttgac ggtatccgat cggcggccgt agccgcgatc ccggtcaaac
    12241 cggacgctat ttagggatcg tcaactgctg cccgggtttg atcttatccg gatccgatag
    12301 tttatcgcga ttggcctcga aaatgcgcat gtaagcattc gcgtcaccgt agaattgctt
    12361 gctgattttc gacaacgagt cgccggcttg cacggtgtag gtttgcgcag cagcctgcgt
    12421 ctgtccggcc gcagccgcct gtccggcctg ctgctccacc cgcagatcgc aggtgaggtc
    12481 ggaataagac gggtcgacca gcttaatctg gtcccatacc ttgtttttgg catcttcact
    12541 gggcgcgacg ccccttatta acagcttatt gttctcaaca tgaacgttct ccaaccggac
    12601 gccttgctgc tcgatcaccc gtaacgccga tccgtatttt tccttgagtt gctgcgtgcg
    12661 ttgttgtttg tcgtctgcca tggtttttcc tggagatggt ttttcctgga gatggtttct
    12721 cctgaggttg gacgcagaat tgcccacttc tgagcaggaa atgagtcctg aaaccgcacg
    12781 gcccggtaaa tcgccgtctt gtcctttcag cgattaaatc acaccattta atggatttcc
    12841 attcatttca tagggttact ggctgaaact gcgtgttgag ctatatccgg acgttctacg
    12901 ggcgctcgcc gatttgaggt gtccgtcgaa gccggagccg ccggcaggaa attgtttcgg
    12961 gccggggcaa attgcgctgg gttctgggca cccggccgtc tggagtcaat aattcgttgt
    13021 cactcgggag ggagcaatat gagcactcag ccaattcatg gaaatcctgc gccggacctc
    13081 gttcaaatcc ggagttcgat tgaagatatc atccatcaac gactggcgga ggagcgggca
    13141 cggctggaac gtgaggccgg actggcgaac catggcgtcc accatttcaa acggcccgtg
    13201 gaacgcacat tcacgaagag cgagcgggga cgtacgacgc tgctgttcgg cggcctgacc
    13261 tggaaacacg aaaagctggt tcacggggcg ctggaaggcc tcggctacct ggcggagacg
    13321 attcccacgc cgaacgtcgc cgcattccaa ctgggtaagg aatacggcaa caacggccag
    13381 tgcaacccca cctatttcac ggtcggaaat ttagtccagt atcttcagca gctcgaagag
    13441 caaggcgttc ccaagcagga aat
//
LOCUS       DASTMK010000203         7953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_329818, whole genome shotgun sequence.
ACCESSION   DASTMK010000203 DASTMK010000000
VERSION     DASTMK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329818"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..519)
                     /locus_tag="VFQ79_08830"
     CDS             complement(172..519)
                     /locus_tag="VFQ79_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139801.1"
                     /translation="MLPKNWVEYLTTLYTPASEVWPRPVQREMGGTVSRWGYGAMWWV
                     WDAPIGTASANWTPFTGSYSAFGTDGQYITVIPSFDLVVAHKNANIDQEPNRNVGMLA
                     YQTILQMLISSKL"
     gene            complement(489..1166)
                     /locus_tag="VFQ79_08835"
     CDS             complement(489..1166)
                     /locus_tag="VFQ79_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0139802.1"
                     /translation="MASPEAAGYSRERLDLLRAWLKTHDTTSMMVVVGGRVLFEYGNV
                     VEISKVASVRKSVLGMLFGKYIAKDPRMLHATVEDLRLEDLESFEQIEKRATLQDLLM
                     ARSGIYWRTADRDSPRKGSIAPGTQFVYNNWDFNAAGTAFEKIAGKGIYEALKTDLAQ
                     PIGMQDYDTAKQKKIQVMPQKKLSVHPEYAMYLSTRDMARLGLLMARHRGGGTVRTCC
                     QRTGWST"
     gene            1503..4190
                     /locus_tag="VFQ79_08840"
     CDS             1503..4190
                     /locus_tag="VFQ79_08840"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU0139803.1"
                     /translation="MPELRKTHKPALTILLLLLPVSLAAQAPWPPLERDHKPWTRWWW
                     HGSAVDRENLTAELERLAAAGFGGVEITTIYGVKGTEARVLPYLSPAWTEMFRFAAEE
                     VARLGLGVDMPPGSGWRTGGPWVRKEHANTSVKISRLQAAPDKPVTVDAGKRLIDAVI
                     AYPERGEPLDLTSRLAEGKLRWAAPDGQWTVYVVERVPSGDSVKRPAPGGEGDAIDVY
                     SPAAFAAFFEAFEQRLAPAERRKVRSWFHDSFEYRGDWSPVFLDEFQKRRGYDLKREI
                     RALVGDESPERSARVLSDYRETISDLLRDNFVERLTAWAHAQGGLNRNQAHGSPGNLL
                     DLYAACDIPETEIFGRLGGPDSNPLINKFASSAAHVTGKRLVSSESYTWLNEHFQTTL
                     AEMKAATDQLFLTGVNHIFFHGTAYSPADAPWPGWVFYASVQANPRNPIWKDIPALTA
                     YIARCQSVLQSGHAGNDVLLYWPYYDVIDNPGPMFQQLSVHSPSWFYQEAIGGIAAEL
                     WRNGFGFDYISDRQLARPGVRAPYKAVIVPACRRMPAATFQALLEAARTGEAVIFQNQ
                     LPDDVPGLARLPEHRAEIEKLKASLRFHLNSTGQMEAAAGKGKILIAANILRSLDAVR
                     IPREPFSEHQIQFIRRSQETGHSYFLRNSGDAPFDGWISPAVKMASAVLMDPMSGASG
                     TVEQRANRQGAAEVRLQLDPGETIILRTYDTGTAPGGPWHYREPAGSAITLTGEWQVR
                     FVEGGPRLPAGYNTRRLESWTVRGGDAEAFAGTARYSLRFEENSSAAGSWLLDLGEVR
                     DSARVRLNGKDVGTLIAHPYRIQTGPLKSAGNLLEVDVTSVGANRIRDMDRRKIPWRI
                     FYDINVVNTKYQPFDASGWPLRPAGLLGPVRLIPLKAGQ"
     gene            complement(4239..4958)
                     /locus_tag="VFQ79_08845"
     CDS             complement(4239..4958)
                     /locus_tag="VFQ79_08845"
                     /inference="COORDINATES: protein motif:HMM:NF038125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THxN family PEP-CTERM protein"
                     /protein_id="HEU0139804.1"
                     /translation="MKQLRWAVVALVVFALCSGQLAAATITISSVTGSWMNPNPAGVT
                     ITNGADMSRVSWGVPIGSGSQSSYVFDSDVPPPLSVEVPPTVTPWLDLGNFTHNNFPI
                     NPPSLSSIELSLVLSMLVDGTPVSQSFLYTLTHNETNNVGPCAFPGMTTCPDQVSIST
                     PSSGTFVAGGVRYTLELRFSRDGGATVLDQFITEENRANTADLFGRFTSELVPIPEPA
                     SMGLIGLGLAGLLLAKRKLTA"
     gene            5150..7024
                     /gene="asnB"
                     /locus_tag="VFQ79_08850"
     CDS             5150..7024
                     /gene="asnB"
                     /locus_tag="VFQ79_08850"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU0139805.1"
                     /translation="MCGIAGYFSLEQPAGLALVKAMCNEIRHRGPDDEGFHIDGGCAI
                     GMRRLSIIDLATGHQPISNETGSVRVVLNGELYNYRELREDLAARGHRFSTQSDTEVL
                     VHLYEEEGLEGLHRLRGMFAFALWDAAEQRLVLARDRFGKKPLYYAVLPKGLYFASEL
                     KCLRVAGVPAELDEQALRLYFYLRYIPEPLTPWRAVKKLTPGGWLVYDQAGTLRTGRY
                     WKLPTPVVTEPAGFSYEDACERVRELFDDSVKVRLMSDVPLGAFLSGGLDSSSVVASM
                     AKQSDRPVRTFSIGFEEASHNELPYAEAVARHYHTEHHSILLRPDAVSLIPRLVRHFD
                     EPFGDSSAIPSFLVSEFAVQQVKVALSGDGGDEAFAGYISFQKLEEFRRYDRIPPPAR
                     KLISALADRLPYHAYGKNFLRMISRPTTLERYFDWNTMPRFLLEQLLQKKWLLPADGA
                     FLFRQWTDNLLPNGADALSQAFYFEATANLTGDMLVKVDRMSMANSLEVRCPLLDHRL
                     VEFAFSLPPHWKMQNGRGKRILVDALGGRLPPELLSLPKKGFSIPLPAWFRGPLREFL
                     CDHLLTPRFRQRGFVSEKFLDYLITEHVSGRRDNATSLYMLLMLEVWMREEESIRATA
                     "
     gene            7032..7280
                     /locus_tag="VFQ79_08855"
     CDS             7032..7280
                     /locus_tag="VFQ79_08855"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEU0139806.1"
                     /translation="MTLHIKNPEAHKLARAIQQVTGETLTRVVTEALRERYERVRKGD
                     PEALSADIRAIAQRASVHIKRPYLDHAEFLYDEHGLPK"
BASE COUNT         1622 a         2422 c         2265 g         1644 t
ORIGIN      
        1 ggttgcgatg cataagagta aagtaagtcg aagcgaacgc attgctgaaa ccagccggaa
       61 gatgattcac agtgcctgca gcgtgaatca aattacaata atacggggcc tccggaaagc
      121 tgcaaacgtg gcccactgcc ggcacgttaa tcaggcatta cgttgtccgc ctcaaagctt
      181 cgacgagatc aacatttgca ggatcgtttg ataggctagc attcccacgt tccgattcgg
      241 ctcctgatcg atgttggcgt tcttgtgcgc caccacgaga tcgaagcttg gaatgacagt
      301 gatgtattgc ccgtcggttc caaacgccga gtaggacccg gtgaacggag tccagttcgc
      361 ggacgctgtt ccgatcggcg catcccacac ccaccacatt gcaccgtagc cccaccgtga
      421 gactgtgccg cccatctcgc gttgaaccgg ccggggccac acttcggaag caggtgtata
      481 cagcgtggtc aggtactcca cccagttctt tggcagcacg ttcttaccgt tccaccgccc
      541 ctgtgcctcg ccatcagaag cccaagacgt gccatatcgc gcgtggagag atacatcgca
      601 tattctgggt gaacggaaag cttcttctgg ggcatcacct gaattttttt ctgcttggcc
      661 gtgtcgtaat cctgcattcc aatcggctgc gccagatccg tcttcagcgc ttcatagatt
      721 cccttacccg ctatcttctc gaacgctgtg cccgccgcgt tgaagtccca gttgttgtac
      781 acgaactgcg tgcctggagc gatgcttcct ttgcgcgggc tgtcgcgatc ggcggtgcgc
      841 cagtaaatgc cggagcgggc catcagcaga tcctgcagcg tggcccgctt ttcgatctgc
      901 tcgaaagact ccaggtcttc gagccgcaga tcctccaccg tagcgtgcaa catcctcggg
      961 tcttttgcga tgtatttgcc gaacagcatg cccagcacgc tcttgcgcac cgacgcgacc
     1021 ttgcttatct ccaccacgtt gccatactcg aagagcacac gcccgccaac aaccaccatc
     1081 atgcttgtgg tgtcgtgcgt cttcagccag gcacgcaaga gatcaagccg ctccctcgaa
     1141 tagcccgcgg cctcgggact tgccattctt tcccattcag ttgccggata ggtggacccg
     1201 gctgggggcg tgtattcgga cggtcgcgtg aagaccagac tgggctcctg acccagggcc
     1261 gcaaccgcaa ccaatgcggc ggtgcatatg tgcaagcgca acatggaccg atgatacacc
     1321 gggcagcgcg cgctgccggc acgtcttgcc aggttgtcat gccatgaagc ttcgcgtgga
     1381 cttccaacct cgttcgagta gccgtgcgat gacaccgaag cgccccacgc gcacgtaaac
     1441 cgcttaaggc tttgcgcaat actctgtatg aagatgttgc acgaatagtt ctcaccattt
     1501 ccatgccaga gttgcgtaaa acccataaac cggcgcttac gatcctgctc ctgctgttgc
     1561 cggtctcttt ggccgcgcag gcaccttggc ctcctctgga gcgggaccac aaaccttgga
     1621 cacgatggtg gtggcacgga agcgccgtcg atcgcgagaa cctcaccgcc gagttagagc
     1681 gattggccgc cgcaggattc ggtggtgtag agatcaccac tatctacggt gtgaagggca
     1741 ccgaggcgcg agtgcttcct tatctttcgc ccgcatggac agagatgttc cgcttcgcag
     1801 cggaggaagt cgcccggctt ggcctgggcg tagacatgcc gcccgggagc gggtggcgaa
     1861 ccggcggccc ctgggttcgc aaggaacacg cgaacacctc ggtgaaaata tcacggttgc
     1921 aggcggcacc ggacaaaccg gtcacggtgg acgcaggtaa gcgcctgatc gacgccgtta
     1981 tcgcatatcc cgaacgcggt gaaccgctgg acttaaccag ccgcctggcc gaaggaaagt
     2041 tgcgctgggc cgcgcctgat gggcaatgga cggtgtatgt agttgagcgg gtgccgtcag
     2101 gcgatagcgt caaacgcccg gcgccgggcg gggaaggcga tgccatcgac gtgtattctc
     2161 cggctgcctt cgcggcgttt tttgaagcat tcgaacagcg gctcgccccg gccgaacggc
     2221 ggaaggtccg ctcatggttt cacgattcct tcgaataccg cggcgactgg tcgcccgtct
     2281 tcctcgacga gtttcagaag cggcggggct acgatctgaa gcgggagatt cgagcgctcg
     2341 tgggcgatga gtcgccggaa cgctcggccc gcgttctgtc cgattaccgc gagacaatct
     2401 ctgatctgct gcgcgacaat ttcgttgagc gcctgacagc atgggctcac gcgcagggcg
     2461 ggctgaaccg caaccaggcg catggatcgc cgggaaacct gctggatctg tatgccgcct
     2521 gcgatatccc cgagaccgaa attttcggac gcctgggcgg accggacagt aatccgctca
     2581 tcaacaagtt cgcctcgtcg gcagcgcacg taacgggaaa gcgcctggtt tcctccgaaa
     2641 gctacacctg gctcaacgag catttccaaa ccactttggc ggagatgaaa gcggccaccg
     2701 atcagctatt cctgaccggc gttaatcaca ttttctttca cggcacggcg tactcgcccg
     2761 cggatgcgcc ctggcccggc tgggtcttct acgcttccgt tcaggcgaat ccgcgcaatc
     2821 ctatctggaa agacatccct gcgcttactg cctacatcgc acgttgccaa agcgtgctgc
     2881 aaagcgggca cgccggcaac gacgttctac tctactggcc ttactacgac gtcatagaca
     2941 atcccggccc gatgttccaa cagttgtccg tgcattcgcc ctcctggttt taccaggagg
     3001 ccatcggagg gatcgcggcg gaactatggc gcaacgggtt cgggttcgac tacatatcgg
     3061 accggcaact cgcccggccc ggagtgcgtg cgccttacaa ggccgtcatc gttcccgctt
     3121 gccggcggat gcccgctgcg accttccagg ctctcctcga ggcagcccgc acgggagagg
     3181 ccgtgatctt ccagaatcaa ctgccggatg acgtacccgg cctcgcgagg cttccggaac
     3241 accgcgccga aatcgaaaaa ctgaaagcca gtctgcgctt ccatctcaac tctacggggc
     3301 aaatggaagc cgcggcggga aaaggaaaga ttctgatcgc cgcgaacatc ctgcgctcac
     3361 tcgacgcggt gcggattccg cgcgaaccgt ttagcgagca ccagatccag ttcatccggc
     3421 gctcgcagga aaccgggcat tcctactttt tgcggaacag cggtgacgcg cctttcgacg
     3481 gttggatttc tccggcggtg aaaatggcgt ccgcggtttt gatggacccg atgagcggcg
     3541 catccggcac ggtcgagcag cgcgcgaatc gccagggagc agcagaggtg cgcctgcaac
     3601 tcgatcccgg cgaaacgata atcctgcgta cttacgatac cggtacagca cccggcggtc
     3661 cctggcatta tcgtgagcct gccggaagtg ccataacgct tacgggtgag tggcaggtcc
     3721 gtttcgtcga gggagggccc aggctccccg ccggatacaa cacgcggcgc ctggagagtt
     3781 ggacggtgcg tggaggtgat gccgaagcgt ttgccggaac cgcgcgctat tccctccgtt
     3841 tcgaggagaa ttcgtcagcc gccgggtcct ggctgctcga tcttggggaa gtgcgcgaca
     3901 gcgcgcgggt acggctgaat ggtaaggacg tgggaacgct catcgcccat ccctaccgta
     3961 ttcaaaccgg tcctctcaaa tcagccggaa atctactgga ggtggatgtc accagcgtgg
     4021 gagcgaatcg catacgcgac atggaccgcc gcaagatccc ctggcggatc ttctatgaca
     4081 tcaacgtggt gaatacgaaa taccagccgt tcgacgcatc ggggtggccc cttcggcccg
     4141 caggacttct ggggccggtg cggctgattc cattaaaggc agggcaatga gaggtgggta
     4201 gagggacgag ggagtgcagg acaccctcgt cttagaactt acgctgtcaa cttacgcttt
     4261 gccaacagca gaccggccag tcccaatcca atgagcccca tcgaggcggg ctccggaatc
     4321 ggcaccagct cggaggtgaa cctgccaaac aggtcagccg tgttggcccg gttttcttcg
     4381 gtgatgaatt gatcgagaac ggtggcgccg ccgtcccgcg agaaccgtag ttccagtgta
     4441 taccgcacac cgccggcaac gaacgtcccg ctagagggag tgctgatact cacctgatcg
     4501 gggcaagttg tcattcccgg aaatgcgcac ggacctacgt tattcgtctc attgtgcgtc
     4561 aacgtataaa gaaacgactg gctcactggc gtaccgtcta ccagcatgct caatacgagg
     4621 ctaagttcga tggaactcag cgacggcgga ttgatcggaa agttgttatg cgtgaaattt
     4681 cccaagtcga gccacggtgt tactgtgggg ggaacttcca cggaaagcgg tggcggcaca
     4741 tcactgtcaa aaacataact gctctgtgag ccgctgccta tcggaactcc ccaggatacc
     4801 ctgctcatgt cggcaccgtt agtaatcgtg acaccggcag ggttgggatt catccacgat
     4861 cccgtaaccg acgaaatcgt gatggttgcc gcggcaagct gaccagaaca caatgcgaac
     4921 acaaccaggg ctactaccgc ccatctaagt tgtttcacga gattcctccg agatgagacc
     4981 tcattttacc tcccaagatc tcttagatga atgtaaaaca taaccatgtc tagttgcaag
     5041 taaaacaagg aagttcaccg gatctttaaa gatcgctggc ggccggaccg gaccggccct
     5101 cgaacgtccg caccccatgt tacagtgggc aacaggtatc tgattctgga tgtgtgggat
     5161 agcgggctat ttttcactgg agcaaccggc cgggctggcg ctcgtaaaag cgatgtgcaa
     5221 cgagattcgc caccgtggcc ccgacgatga aggttttcat atcgatggcg gttgcgccat
     5281 cggcatgcgc cggctcagca tcatcgatct tgccaccggc catcaaccta tctcgaatga
     5341 aaccggctcg gtacgggtgg tcctcaacgg cgaactgtac aactacaggg aactgcgtga
     5401 ggatctcgct gctcgtggcc atcgtttctc gacgcaatcc gacaccgaag tacttgtcca
     5461 tctgtatgaa gaagaaggcc tcgagggact ccaccggctg cgcggaatgt tcgctttcgc
     5521 cctgtgggac gcggccgagc agcgcctcgt gctggcacgg gaccgtttcg gcaaaaagcc
     5581 gctttactat gccgttctgc cgaaagggct ctatttcgcc agcgagttga agtgtctgcg
     5641 cgtggcgggc gtgccggcag agctggacga gcaggcgctc cggctctact tttatctgcg
     5701 ctatattccc gagccgttga ccccctggcg ggcggtgaag aaactgaccc caggcggctg
     5761 gctggtctac gatcaggcgg gcaccctacg caccggacga tattggaagc tgccgacgcc
     5821 cgtggtcacg gagcctgccg gcttcagtta tgaagatgcc tgtgaacgcg ttcgggagct
     5881 tttcgacgac tccgtaaaag tccgcctgat gtcggacgtg cccctgggtg ccttcctcag
     5941 cggggggctg gattcgtctt cggtcgtcgc gtccatggcg aaacagtcgg accggcctgt
     6001 gcgcaccttt tccatcggct tcgaagaggc cagccacaac gagctgccct atgccgaggc
     6061 cgtagcgcgc cattatcata ccgagcatca ttccatcctt ctccggcccg atgccgtcag
     6121 cctgattccc cgcctggtgc ggcacttcga tgaaccgttc ggcgattcct ccgccatccc
     6181 ctcgtttctg gtctccgaat tcgccgtaca gcaggtaaag gtggcgctta gcggcgacgg
     6241 cggcgatgaa gcgttcgccg gttacatcag cttccagaaa cttgaagagt tccgccgcta
     6301 cgaccgtatt cccccacccg cgcgcaagct gatttccgcc ctggcggacc ggctgcctta
     6361 ccacgcctac gggaagaatt tcctgcgcat gatcagccgg cccacgacac tggagcgcta
     6421 tttcgactgg aatacgatgc cacggttcct gctcgaacaa cttctacaga agaaatggct
     6481 gctgcccgcc gatggcgcgt tcctgtttcg ccagtggacg gacaacctgc tgcccaacgg
     6541 ggccgatgcc ctgtcgcagg ctttctattt cgaggcaacg gccaacctta cgggcgacat
     6601 gctggtgaag gtcgaccgta tgtccatggc caactcgctc gaggtgcgct gccctcttct
     6661 cgaccaccgc ctggtggaat tcgctttctc gctgccgcct cattggaaga tgcagaatgg
     6721 gcgcggcaag cgcatccttg ttgacgccct cggcggccgc ctgccccccg aattactgtc
     6781 gctccccaag aaaggcttca gtattcctct accagcctgg ttccgcggcc cgctgcgcga
     6841 atttctctgc gaccacctgc tgacgccacg tttccgccag cgcggcttcg tatcggaaaa
     6901 attcctcgat tatctcatca cggagcacgt gtccggccgc cgcgacaatg ccacgtcgct
     6961 gtacatgctg ctcatgctcg aagtctggat gcgcgaagaa gaatctatcc gcgccacagc
     7021 ctgatagatt aatgaccctc catatcaaaa accccgaagc ccacaaactg gcccgggcga
     7081 ttcagcaggt gaccggggaa acacttacgc gcgtcgttac ggaagctctc cgggaacgct
     7141 atgagcgtgt ccggaaaggc gatcctgaag cgctctcggc tgacatccgc gccatcgcac
     7201 agcgtgcttc ggtccatatc aagcggccct atctggacca tgctgagttt ctttacgacg
     7261 aacacggcct cccaaaatga tcctggattc ctccccgcgc ttcgcgctgc cgtattttgg
     7321 gaggcaactt tatcgcggtc tcaatcgtgc tttttcgccg cgccgtcatc gagataaaac
     7381 ctgtgactgt cgagcaggca catattgcac gccaggcatt tcacaatttc ggcaaggggc
     7441 gccacctggc ccgcgttgaa cttcgggggt tgcttcgtat acgctctcgc caaacatacc
     7501 ggtgaacggc aagcgcatcc tcgtcgacgc cctcagcggc cgtctgcccc cgaattgctg
     7561 tcgcttccca aaaaaggctt ccgccagtgc ggtccggacg tcgccgcgat aacgccacgt
     7621 cgctatacat gctgctcatg ctcgaagtct ggatgcgtga cgaagagatc atcggcgaac
     7681 aatttacaat aaaatggcct gaatatcctt gattctagcc tcgaataggt ccaaactacc
     7741 cgtagttttt cctatgggac gagagattgc agtagagatt ccgtcgagaa cgctctacat
     7801 gctggcccga aaattcatgt tgccaggagc ataggagtcg aaagagttta tgaaattgag
     7861 catgaacata caatttctca agcgggtatt tttacccctt ttcgtgatga catcgcttta
     7921 tcaaaacgct ggagtcgtcg cgggtttggt ctc
//
LOCUS       DASTMK010000204        10538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_618222, whole genome shotgun sequence.
ACCESSION   DASTMK010000204 DASTMK010000000
VERSION     DASTMK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618222"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..881
                     /locus_tag="VFQ79_08860"
     CDS             141..881
                     /locus_tag="VFQ79_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139807.1"
                     /translation="MPKPALQFYGEPLPGIDSAELQGKLIVVEGPDAVGRSTQIAMLR
                     GWLEQEGHAVLDTGLARSALAGKGIQRAKEGNTLGPITMTLFYATDFADRLENEIIPA
                     LRAGFVVLIDRYIFSVMARAIARGEDRAWIEKVSGFALVPHAVYYLKADVQDLIKRVV
                     VGRGAFDHWESGMDMRFGSDMYDSFVKYQARLIRALDSMAAKYDFEIVDARRTPEEIF
                     ADLQKRITALFRPRRSSGTGRSSDTIQS"
     gene            878..1627
                     /locus_tag="VFQ79_08865"
     CDS             878..1627
                     /locus_tag="VFQ79_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0139808.1"
                     /translation="MNSPFRLDGRTALVTGGASGIGEAISRVFSDAGASVLIVDIDKS
                     RADALAAELPRAAVHDCDITNEEAVQALFGGLTGLDILVNNAGIGMVGSIEETPLEDF
                     QRLFRVNVEGLFLMTRYAMPLLLESHGSIVNIGSVAGLVGIKKRFAYCATKGAVVAMT
                     RQVAVEYAGRLRANCICPGTVDTPFVEAYLEKYHKHEKEKIRAELNQRQPVGRLGRPM
                     EVAHLALYLSSQEAEFATGTIATIDGGWTAA"
     gene            1850..2890
                     /locus_tag="VFQ79_08870"
     CDS             1850..2890
                     /locus_tag="VFQ79_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU0139809.1"
                     /translation="MKAGVAANPARKRRITYSRWTEPNYADSAAGDILAGEDLVVRQA
                     ALAALGLLNGTVVVVDPNTGRILTIVNQKLAFQSGFQPCSTVKVFTALAALSEGLIGR
                     TSPIRVSKRTSMNLTEALAVSNNPYFAALGQKLGFERVRYYGRLFGLGETAGLGIAEE
                     EPGILPEMPPANGGVGMMTSFGEGITLTPLELASLLSAIANGGTMYYLQHPRSQAELD
                     YFIPRVKRNLDIQPFIPEVKPGLLGATEFGTARRASYSADEPIYGKTGTCTDRRSSGT
                     HLGWFGSFNEVGGKKLVVVVLLTGGSAVSGPVASGVAGAVYRNLSMSQFFARESTPSP
                     ATLNVTGTCCVE"
     gene            complement(2897..3901)
                     /gene="obgE"
                     /locus_tag="VFQ79_08875"
     CDS             complement(2897..3901)
                     /gene="obgE"
                     /locus_tag="VFQ79_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU0139810.1"
                     /translation="MFIDQVRIHIKAGDGGNGCLAFRREKYVPRGGPSGGDGGNGGDV
                     VLVSNENYNTLLHFRFNPEYSAERGRHGEGSNRKGRDGQSIEIPVPPGTVVYDADTGE
                     LLEDLTAAGQRLVIAKGGRGGRGNARFATSTHQAPTEHEPGKPGQERNLLLELKLLAD
                     VGLVGFPNAGKSTLISRISAARPKIADYPFTTLEPGLGVVRLDDDRTFVVADIPGLIE
                     GAHKGAGLGIQFLRHVERTQVLAHLVDASEASGRDPVHDFDVIMEELGSFSAALAEKP
                     MILVANKIDATQDTSRIDALRQLAGERQLPFFAISAVTGKGVDQLLYALAAAVSAKVR
                     "
     gene            complement(3962..4219)
                     /gene="rpmA"
                     /locus_tag="VFQ79_08880"
     CDS             complement(3962..4219)
                     /gene="rpmA"
                     /locus_tag="VFQ79_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU0139811.1"
                     /translation="MAHKKGLGSSKNGRDSNAQRLGIKAFGGQTVSGGSILVRQRGTK
                     YKPGENVGRGKDDTLFARITGIVEFRDRGRMGKFISIREQA"
     gene            complement(4228..4539)
                     /gene="rplU"
                     /locus_tag="VFQ79_08885"
     CDS             complement(4228..4539)
                     /gene="rplU"
                     /locus_tag="VFQ79_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU0139812.1"
                     /translation="MYAVIQTGGKQYRVAPGQTIEVEKLPGDAGTQVELGQVIAVMKD
                     SDELVAGPDLGDAKVTATIAGHGRGSKVVVFKFKRKKQYKRTIGHRQSYTRLKVDDIV
                     I"
     gene            complement(4918..7575)
                     /locus_tag="VFQ79_08890"
     CDS             complement(4918..7575)
                     /locus_tag="VFQ79_08890"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0139813.1"
                     /translation="MSNRTRRRFLGTVGSGVPTLSLVSQAAGGMVFGQEGGASGRTSP
                     KFTPVDLSRFFNAGPAGFGPRDRARRLSGPSRADGLIRMPTGEQRFRGIPFRLGPEGA
                     AGSSWIWLSTQPGRAVSEVEIPLSNKAGFICFAHFCDWDPHETPDFDAVTVEQVGQTL
                     AEAVLIYSGGGEAAFPIRRRIEVNAPSITWGFPGYACVSHYQDSARKSRNELDEVGDW
                     GWFRLQSDVLEGNYTVDASTGAPKPSLWLFALPNPHPDRRLSALRLRAAGQDPLLVCG
                     VTLFHGRQHPLRYERLTLYRLTLPDASGQWSTEVDLGVVARSYHLGKFDPQEWLDAPD
                     AGLGSRTTAVDTRHLYIEVTASSEATLLLRDGRGGRQFEFALGQVTAGKEMPGTPAGS
                     RIEILETAKTWIHGSVADAATGRPTPVRLAFRSSDGRYIPPYGHRTEVDAGTFKDWGA
                     DLKLADTCYAYVDGTFQIELPVGDVYVEISKGFEYEPIRQRLAIQAGQRELKLEIART
                     LDFRSRGWVTADTHVHFLSPSTAVLEGQAEGLNIVNLLATQWGDLFTNVGDLSQGPLA
                     SSDGEMVVWMGTENRQHILGHLAVLGKSVFPMAAGGPRESYHGDPVWDTLAGWSDAAR
                     EQGGLAVSVHFPYPAGEQAADIVLGKTDAVELFPDTGLRLQHWYRALSCGYRLPVVGG
                     TDKMGAGTAVGANRTYAQMGVEPLNSTSWARAVRGGNTFMTTGPLLLFEADGRVPGQE
                     IRLGQGGGTIEVLAHARSVRPIHRVDVVWNGRVVASRQNREGARDITLKERIQVPGPG
                     WLAARCSSTLRPRIMAHTSPVYAQVPGQEVFSKTAAAYMLTQVEAVQTWVETLATRPD
                     PERFEKIRKVFADARTILHRRLHEHGVKH"
     gene            complement(7663..7788)
                     /locus_tag="VFQ79_08895"
     CDS             complement(7663..7788)
                     /locus_tag="VFQ79_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139814.1"
                     /translation="MSVSQITDNRNRSYGGCATVLYDDTVRQNLVEKLVGYPSRE"
     gene            complement(8006..9607)
                     /locus_tag="VFQ79_08900"
     CDS             complement(8006..9607)
                     /locus_tag="VFQ79_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU0139815.1"
                     /translation="MSRLFIALLLAATAYSVDPVSANRIRAHTKFLSSDLLEGCGVGT
                     RGDKLTTEYLATQLALAGAKPAGDNGTYFQKVPLIGVTTEPSARLSAVSTEQTIEFRW
                     LQDFVGASHRQGNLTEFQTEAIFVGHGIVAPEQGWDDYKGTDVRGKIVVLFTNEPQSD
                     NPALFGGRALTYYGRWTYKYEEALRQGALGAIIIHTDATAGYGWEVVRNSWAREDPQV
                     ELKPGQAALGFAGWLTREAGERLMGLARLKIDELLKLSGSRDFRPIAMGVRIKGRIPA
                     KIRPVETRNVAALIPGSDPALRDQAVLFSAHWDHLGIGEPVDGDAIYNGAVDNATGCA
                     IVLELARAWASLQEKPRRSALFLFVTAEESGLRGSEYYSKNPLMAPGNTAVALNFDGF
                     RPFGRTRDIVVSGAERTTLWPAVQEAAARMNFEIKPDPRPEQGLYYRSDHFSFARVGI
                     PAFSVSLGSEYLGKPAGYGEQLFREFNTRSYHQPSDEFDENWDFSGLEAVARFGFLLG
                     INTANEPSLPAWNEGDEFQKAREAK"
     gene            complement(9610..>10538)
                     /locus_tag="VFQ79_08905"
     CDS             complement(9610..>10538)
                     /locus_tag="VFQ79_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0139816.1"
                     /translation="PEDKQWFGDITKAVVHDEQDKGSYTRIRLDLPMEKDFYQQHLLL
                     LPKGQGDGPFPAVIAWTSSTPDYAKPEEWWGTWLAERGYVVLTSWSFIRNYRDGSTAR
                     NGAPEKVYERFGHWLSIAKMVHDAQRDAEYLRSLKQVDGGRIGFIGFSLSAKTAIYVA
                     AFAPEIHAVVALDPHIAVNGGTNWYAPWYLDWLRPFPNIPTKEKTVLSLLNPDPERPG
                     FEHDHHELLALAAPKPFLLIGGSQSEDGGGDSDDLQSWGYVNRAREVYKLIGAEDHVQ
                     FVSTGDGHKANGPNIDPAWQGFFERWLKRKEQ"
BASE COUNT         2057 a         3251 c         3124 g         2106 t
ORIGIN      
        1 tatgattccc gaaatgggct tccatgtgat cgacgctacc gggtccatcg aagagcagca
       61 gaagcacatg cgcgagattg tgatgaagga attaggggag agcctcgcga ccggggtgct
      121 gcacgtcaag gagagcagac atgccgaaac cggcgcttca attctacggt gagcctcttc
      181 cgggcatcga ctccgccgag cttcagggca agttgatcgt cgtcgaaggg ccggatgccg
      241 tcggacgatc cacgcagatc gccatgctcc gcggatggct cgagcaggaa ggtcacgccg
      301 tgctcgacac cggccttgca cggtccgctc tggcaggaaa gggcatacaa cgggctaagg
      361 aaggaaacac gttggggccg atcaccatga cccttttcta cgccaccgat tttgcggatc
      421 gcctggagaa cgaaatcatc cctgctctgc gcgccggttt tgtggtgctg atcgaccgct
      481 acatcttctc cgtcatggcc cgcgccatcg cgcgcggcga ggatcgtgcc tggatcgaga
      541 aggtatccgg atttgccctg gtgccccacg ccgtttacta cctgaaagcc gacgtgcagg
      601 atctcattaa gcgggtggtc gttggacgcg gcgcgttcga ccactgggag agcgggatgg
      661 acatgcgctt cggctcggac atgtacgata gcttcgtcaa atatcaggcc cggctcattc
      721 gcgcgctcga cagcatggct gccaaatacg attttgagat tgtggacgca cggcggacgc
      781 cggaagaaat tttcgccgat ttgcaaaaac gcatcaccgc cctgttccgg ccccggcgca
      841 gctcaggaac gggccgctca tcggatacaa tccaatcgtg aactcacctt ttcgcttgga
      901 tggacgcacg gctctcgtca cgggcggagc cagcggaatc ggcgaagcca tatcccgtgt
      961 tttctcggat gcgggagctt ccgtcctcat cgtagacatc gacaaatccc gcgcggatgc
     1021 gctggctgcg gaactgccac gcgccgctgt gcatgactgc gacatcacaa atgaagaagc
     1081 cgtgcaggcg ttattcggcg gcctgaccgg attggatatt ctcgtgaaca acgccggaat
     1141 cggaatggtc ggcagcatcg aggagacgcc gctggaggac ttccagcgtc tcttccgcgt
     1201 taacgtcgag gggctgtttc tgatgacgcg ttacgccatg ccgctactgc tggaatctca
     1261 tggctccatc gtcaacatcg ggtcggtggc cggcctggtt ggtatcaaga agcggttcgc
     1321 ttactgcgcc actaaaggcg ccgtcgtcgc gatgacgcgg caggtagccg tggaatatgc
     1381 cggccggctg cgggcgaatt gcatctgccc gggaacggtg gacaccccct ttgtcgaagc
     1441 gtatcttgag aagtaccaca aacatgaaaa agagaagata cgtgccgagc tgaaccagag
     1501 gcaaccggtg ggcaggctcg ggcggccgat ggaggtggcg cacctggcgt tgtacctcag
     1561 ttcgcaggag gcggagttcg ccaccggaac gatcgcgacc attgacggtg gatggactgc
     1621 agcttgacgc cggcagtgcc gatcttaaag aatgagtgct cgagttgagc cctgactgag
     1681 cgcgtatttg gaggccatga ggttacagat catcgcccga cttgtgccgg ccgcatgttt
     1741 gattgcgctg ctggccggcc ctttgccgtc agccgagacc cggtcaggtc cgggtactgc
     1801 ctctggaaag gcgcgggcgg cggcaagtac ctccaaatcc gggaaaaagg tgaaagctgg
     1861 agttgcggcg aaccccgcgc gcaagaggcg catcacctac agccgatgga ctgagccgaa
     1921 ctacgccgac tccgcggccg gcgacattct ggctggggag gatctcgtcg tccggcaagc
     1981 ggctttagcg gctctgggtc tgctcaacgg caccgtggta gtggtggatc cgaacaccgg
     2041 ccgtatcctg accatcgtca atcagaagct cgcgttccaa agcggatttc agccctgttc
     2101 cacagtgaaa gtgtttacgg ccctggccgc tttgagcgag gggctcatcg gacggaccag
     2161 cccgatccgc gtctccaaaa gaacaagcat gaacctcacg gaggcgcttg cggtctccaa
     2221 caacccctat ttcgcggcgt tggggcaaaa gcttggcttt gagcgtgtcc gctactatgg
     2281 ccgcctgttc gggttgggtg agactgcggg gctcggaatc gcggaggaag agccgggaat
     2341 tctgccggaa atgccgccgg ccaacggtgg tgtcggtatg atgacaagct tcggggaagg
     2401 catcacgctg acgccgctgg aactcgcatc cctgttgtcg gccatcgcga acggcggcac
     2461 gatgtactac ctgcaacacc cccggtcgca agcggaactc gactacttta taccgcgagt
     2521 gaagcggaac ctggacatcc agccgtttat tcccgaagtg aagcccggcc ttctcggagc
     2581 cactgagttt ggaacggcgc ggcgtgcgtc ttatagcgcc gatgagccca tatacggcaa
     2641 gacggggacc tgcacagacc gccgctcgag cgggactcac ctcggatggt ttgggtcctt
     2701 taacgaagta ggcggaaaga aattggtggt cgtggtgctg ttgaccggcg gaagcgccgt
     2761 gagcggcccc gtggcttccg gtgtggccgg agcagtctac cggaacctgt ccatgagcca
     2821 gttcttcgcg cgggagtcga cgccttctcc ggctaccttg aatgtcacag ggacctgttg
     2881 cgtcgaatag gcggtctcag cgaacttttg ccgaaacggc ggcggccagc gcgtagagga
     2941 gttgatcgac gccctttccg gtgacggcgg agatcgcgaa aaaaggcaac tggcgctcgc
     3001 cggcgagttg ccgcaacgcg tcgatgcgcg acgtatcctg cgttgcatcg atcttgttcg
     3061 ccaccaggat catcggcttc tccgccagtg ccgcgctgaa gctgccaagc tcttccataa
     3121 tcacgtcgaa atcgtgcacc gggtcgcgtc ctgatgcctc ggaggcatcc acgaggtgag
     3181 cgagaacttg cgtgcgttcc acgtggcgaa gaaactgaat ccccagcccc gcgcctttgt
     3241 gcgctccttc gatcaggccc ggaatgtccg ccaccacgaa cgtgcgatcg tcatcgagac
     3301 gcaccacccc gagaccgggc tcgagcgtcg tgaacgggta atccgcaatt ttcggccgtg
     3361 cggcggaaat cctggagatc agagtcgatt tgccggcgtt aggaaacccg accaggccga
     3421 catcggcgag cagtttcaac tccaggagga gatttcgttc ctgtcccggc ttaccgggtt
     3481 cgtgttcggt gggtgcctgg tgcgtggagg tagcgaagcg ggcattcccc cgcccgcccc
     3541 tgcctccctt cgcgataacc aggcgttgcc cggctgcggt gagatcttcc agcaactctc
     3601 cggtgtccgc gtcatagacg accgtgccgg gcgggacggg gatctcgatg gactggccgt
     3661 cccggccctt tcggttgctc ccctcgccgt gcctgccgcg ttcagcgctg tattccgggt
     3721 tgaaccggaa atgtaagagg gtattgtagt tttcattgga gaccaggacc acatcccctc
     3781 cgttaccgcc atcaccgccg ctcggcccgc ctcgcgggac gtacttctcc cgtcgaaacg
     3841 cgagacaccc gttccccccg tctccggctt tgatgtggat gcgaacctga tctatgaaca
     3901 cggcaaaaaa gaaaaggccg ccggaatacc cggcggcctc acggtggaga agtggccttc
     3961 ttcaggcctg ctctcgaatg ctgatgaact tacccatccg gccgcggtca cggaattcga
     4021 caataccggt gatcctggcg aaaagggtgt cgtcctttcc ccggccgaca ttttcgccgg
     4081 gcttgtattt cgttccccgc tgccgtacca ggatagaacc acccgaaacc gtctgcccgc
     4141 caaaagcctt gataccgagc cgttgggcgt tcgaatcgcg gccattcttt gagctgccta
     4201 aacctttctt gtgcgccata gaagctccta aataacgatg tcgtccactt tcaggcgcgt
     4261 atagctttga cggtggccga tagtccgctt atattgcttt ttgcgcttga acttaaaaac
     4321 gacaactttg ctgccgcggc cgtgccctgc aatcgtcgct gtcaccttgg cgtcacccag
     4381 atccggaccg gctaccagtt cgtcagagtc cttcattaca gcgataacct ggcctagctc
     4441 tacctgtgta ccggcatcgc ccggcagttt ctccacttct atcgtttgtc ctggagcgac
     4501 acgatattgt ttgccgccgg tttgaatgac tgcgtacatt aggaactctc tttcgtcaga
     4561 tcgggagcga tcacaaggac ctacacgcac tcatacacgt gcaggcgtaa gccgcaagca
     4621 gccagtgtag cacttcgcgg cgatggatgt aaaccagagt tgagcggttc tctccagacc
     4681 caggagtggg gcatacagca cacctgcggc gaaatgcccc aggcattttg cccaagacgg
     4741 cattcggctg tcatcaaaac atgcgaaaac cgaggaatcc cagtttggca agctgaatgc
     4801 aaataataaa gacaactact aagtcatcat tgacttaagc atcccaacgg ctagtcccag
     4861 ctagtcgttt agcccctctg agtgaagccc cgggtcccca cccggggctt ttctctatca
     4921 gtgcttcact ccatgctcgt gcagcctacg gtgcaggatg gttcgcgcat ctgcaaaaac
     4981 cttacgtatc ttttcgaagc gttccggatc aggccgcgtg gccagcgtct ccacccaggt
     5041 ctgcacagcc tctacctgcg tcagcatgta agccgcggcc gttttggaga atacctcctg
     5101 cccgggaacc tgtgcataaa ccggagaggt gtgcgccatg atccgggggc gtagggttga
     5161 ggagcagcgg gcggccagcc aacccggtcc gggtacctgg atacgctctt tgagtgtgat
     5221 atcgcgtgct ccctcgcggt tctgtcgcga tgccaccacg cgtccgttcc acacaacgtc
     5281 cacgcgatgg ataggcctta cggaccgggc atgcgccaaa acctcgatcg tgccgccacc
     5341 ctgccccaga cgtatctcct ggcctggcac tcgaccgtcc gcctcaaaga gcagcaatgg
     5401 gccggtagtc ataaatgtat tgccgccacg cacggccctg gcccaactgg ttgagttcag
     5461 cggctcgact cccatttggg cgtaggtgcg gttggcgccg acagcggtcc ccgcgcccat
     5521 tttatcggtt ccacccacga ccggcagtct gtagccgcaa ctcagcgcac ggtaccagtg
     5581 ctgaagccga agaccggtgt cgggaaagag ttctaccgca tcggtcttgc caagaacgat
     5641 gtccgccgcc tgctcgcccg ctgggtaagg gaagtgaacg gatacggcca gacctccctg
     5701 ctcgcgtgcg gcatcggacc agccggccag ggtatcccac acggggtcgc cgtggtagct
     5761 ctcccgcggg cctccggcgg ccatgggaaa cacggacttg ccgagcacgg ccaggtggcc
     5821 gagaatgtgt tgcctgttct cagtgcccat ccagaccacc atctcgccgt cggatgacgc
     5881 cagtgggccc tgcgacaagt cgcccacatt ggtaaagagg tcgccccatt gcgtggccag
     5941 gaggttgaca atattcaatc cctcagcctg tccttccaat accgccgtcg atggcgagag
     6001 aaagtggacg tgagtgtccg cggtcaccca gccgcgcgaa cggaaatcca gcgtgcgcgc
     6061 gatctccagt ttcagctcgc gttgcccggc ctggattgcc agtctttgcc ggatgggctc
     6121 gtattcgaat cccttgctga tttcgacata cacatcgccg acgggaagtt cgatttggaa
     6181 cgttccgtcc acatatgcat aacatgtgtc cgcgagttta agatcggcgc cccagtcttt
     6241 gaaggtgccg gcatcgacct cggtacgatg gccgtaaggg ggtatgtagc ggccgtcgct
     6301 ggagcgaaac gccaggcgta caggcgtggg tctgcccgta gctgcgtcgg ccacgctgcc
     6361 atgaatccag gtcttcgcag tttcaagaat ttcgatgcgc gatcccgcgg gggtgcctgg
     6421 catttccttg ccagcggtga cctgacccaa agcgaactcg aactgccgcc cgccacggcc
     6481 gtcccgcaac aacaaggtgg cctcggaact ggcggtcacc tcgatgtaca gatgccgggt
     6541 gtcgacggcg gtcgttcgag agcctaaccc ggcatcgggc gcgtccaacc attcctgtgg
     6601 atcgaacttg ccgaggtgat acgaacgtgc caccacgccc aaatcgactt ctgtcgacca
     6661 ctgccccgaa gcgtccggca aggtcaggcg gtagagcgtt agccgctcgt agcgcaaagg
     6721 gtgttgccgg ccgtgaaata gcgtaacacc gcaaaccagc agcgggtcct ggccggccgc
     6781 gcgcagccgc agggccgaca gtctgcggtc cggatgcggg ttcggcaacg caaacagcca
     6841 gagactcggc tttggtgcgc cggtggaagc atccaccgtg taattgcctt ccagaacatc
     6901 cgactggagc cggaaccaac cccagtctcc gacttcatcg agctcattcc tggatttgcg
     6961 cgcgctgtcc tggtaatggg aaacacaggc gtagcccgga aagccccagg tgatcgatgg
     7021 agcgttcacc tcgatacggc gccggatcgg gaaagccgct tcgccacctc cggaatagat
     7081 gagaacggcc tcagccagag tctgtcctac ctgctctacc gtcacggcat cgaaatccgg
     7141 agtttcgtgc gggtcccagt cgcaaaagtg ggcaaagcag atgaatccag ccttgttgct
     7201 cagcggtatt tccacctcgg agaccgctct cccaggctgc gtgctcagcc agatccagga
     7261 acttcccgcc gctccctccg gccccaaacg gaatgggata ccgcgaaagc gctgctctcc
     7321 cgtgggcatg cggatcagcc cgtcggcccg cgacgggccg ctcagtcttc tggccctgtc
     7381 gcgtggaccg aagccggccg ggccggcgtt gaaaaagcgg ctcaggtcca cgggcgtgaa
     7441 cttcggcgat gtgcgcccgc tcgcgccgcc ctcctgcccg aacaccatgc caccagctgc
     7501 ctgggaaacc aaagagagcg tgggtacacc ggatcccaca gttccgagaa agcgtcttct
     7561 tgtccgattg ctcatctgtt cacagcctct acggcgctcc gttataacac ggtagtctga
     7621 ctcacgtcga gagatctcga atgcgccaag ggcgctgagg aactactcac gggagggata
     7681 acccacgagc ttctcgacca ggttctgccg tactgtgtcg tcgtacagaa cggtggcaca
     7741 accgccatag ctgcgattac ggttgtccgt gatctgagaa acgctcacgg acaacccatc
     7801 tcccaccagt tccgcctcag caccgagttc aagaagtacc tttcgcaggt actcgatcgc
     7861 cctgaaaagt ccattagttc tcagttggaa actgatcagt atgaccggcg ccaacagatg
     7921 ctcgcgcagc taatgttacg cgatgggtgg gtttttcgtg atgttttgcc tgatgccgga
     7981 cagcttatgg accaaatcag gcgccctact tagcctcccg cgctttctgg aattcgtctc
     8041 cctcgttcca ggccggtagc gatggttcgt tagctgtgtt tataccgagc agaaagccga
     8101 accgggccac cgcttcgagt cccgagaagt cccagttctc atcgaactcg tccgagggct
     8161 gatgatagct cctggtgttg aactcgcgaa acaattgctc gccgtagccg gcgggtttgc
     8221 ccagatactc gctgcccagg ctgacggaaa acgccgggat cccgacgcgt gcgaaagaga
     8281 agtggtccga gcggtaatag aggccctgtt ccggccgggg atcgggcttg atttcgaagt
     8341 tcatccgggc agcggcttcc tgaaccgcgg gccagagagt ggttcgttcc gcgccggaaa
     8401 caacaatatc cctggttctc ccgaatggcc ggaaaccatc gaaattgagg gccacagccg
     8461 tgttgccggg cgccatcagg gggttcttcg aatagtactc ggacccgcgc agcccgctct
     8521 cttccgccgt cacgaacaag aagagcgccg agcgcctggg cttttcctgg agcgatgccc
     8581 aagccctcgc cagctccagg acgatggcac agcctgtggc gttatcgacg gcaccgttgt
     8641 aaatcgcatc cccgtccacg ggttctccaa tcccgaggtg gtcccagtgc gcgctgaaaa
     8701 ggacggcctg atcccgcaac gccggatcgc tgcccggaat caaggctgcg acattgcgtg
     8761 tttcgacagg ccggatcttt gccggaatgc ggccctttat gcgaacgccc atggcaatgg
     8821 gccggaagtc ccgcgaaccc gatagcttca gcagttcatc gatcttcaga cgcgccaggc
     8881 ccatgagccg ctctcccgcc tcgcgggtca gccatccggc gaaaccgagc gccgcctggc
     8941 ccggttttag ttcgacctgc ggatcttcgc gcgcccagga gttgcgcacc acctcccatc
     9001 catagcccgc cgtcgcgtcg gtatgaatga tgatcgctcc gagagcgccc tgtcgcagcg
     9061 cctcttcgta tttgtaggtc caccggccat agtaggttag cgcccgccct ccgaatagag
     9121 ccggattgtc agactgcggc tcgtttgtga agagcacgac gatcttgccc cggacgtccg
     9181 tgcctttgta gtcgtcccac ccctgttccg gggccacaat gccatgcccg acaaatatgg
     9241 cttccgtctg gaattccgta agattgccct ggcgatgcga cgcaccgacg aagtcctgaa
     9301 gccagcggaa ttcgatggtc tgctctgtcg acactgcgga gagacgggcg gagggctctg
     9361 tcgtaacccc gatcaaaggc accttctgaa aataagtgcc gttgtctcct gccggtttgg
     9421 cgccggcaag agcgagttgc gtggccagat actccgttgt gagtttgtct cctcgggtgc
     9481 cgacgccgca gccttccagc agatcgctcg acaaaaactt cgtatgcgcg cggatgcggt
     9541 tggccgagac cggatctaca gaataggccg tggccgccag cagaagcgcg atgaacagcc
     9601 tgctcatgac tattgctctt tccgtttcag ccagcgttca aaaaatccct gccaggccgg
     9661 atcgatgttg ggtccgttcg ccttgtggcc gtccccggtg gaaacgaact ggacatggtc
     9721 ctccgcgccg atcagcttgt acacttccct ggcgcgattg acatatcccc agctttggag
     9781 atcgtcggaa tcgccgccgc cgtcttcgga ttggctgcca ccgatcagaa gaaacggctt
     9841 gggagcggcc agcgccagca gttcgtgatg gtcatgctcg aagcccggcc tctccgggtc
     9901 gggattcaga agactgagaa cagtcttctc ctttgtcggg atattcggga agggccgcag
     9961 ccagtccagg taccagggcg cgtaccagtt ggtgccgccg ttcaccgcaa tatgaggatc
    10021 cagcgccacc acggcgtgga tctccggcgc aaaagcggca acatagatcg ccgtcttggc
    10081 gctcaaagaa aatccgatga agccgatcct cccgccgtcc acctgtttca ggctgcgtaa
    10141 atactccgcg tcgcgctgcg cgtcatgaac cattttggcg atggaaagcc agtggccgaa
    10201 acgctcatat accttttcgg gcgccccgtt tcgtgccgtg ctcccatccc ggtagttgcg
    10261 gatgaaggac cagcttgtca gaaccacgta gccgcgctcg gccaaccacg tgccccacca
    10321 ctcttccggc ttggcgtaat cgggagtact cgaagtccac gcgatgacgg ccggaaacgg
    10381 accgtctccc tgtcccttcg gcagcagcag gaggtgttgc tgatagaagt ctttttccat
    10441 tggcaggtcg agacggatgc gcgtgtagct gcctttatcc tgctcgtcgt gaacgacggc
    10501 tttggtgata tctccgaacc actgtttgtc ttccggcg
//
LOCUS       DASTMK010000205        18370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_106108, whole genome shotgun sequence.
ACCESSION   DASTMK010000205 DASTMK010000000
VERSION     DASTMK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18370
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106108"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="ffh"
                     /locus_tag="VFQ79_08910"
     CDS             complement(<1..1146)
                     /gene="ffh"
                     /locus_tag="VFQ79_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU0139817.1"
                     /translation="MFDTLSDRLSGIFTKLRGKGRLTDADIDATAREIRLALLEADVA
                     LPVVKSFIFNLKERARGAEVSQALNPAQQIIKIVHEELINVLGGEGRRLQFAKQPPTV
                     IMLAGLQGSGKTTLAGKLARWLKGQGHQPLLVAADLQRPNAVNQLQVLAGRAGVDVFA
                     PQPGNGVGDPVEVARESIEHARRTMHDVVVVDTAGRLGIDAELMAQAAAIRDAVRPDE
                     TLFVVDAMIGQDAVTTATAFQEGVGFSGVVLTKLDGDARGGAALSVRYVTGQPIMFAS
                     TGEKLADFDVFHPERMASRILGMGDVLTLIEQAEQAFDEEQAERMAGKLASREGFTLE
                     DFLEQMMAIRKMGPIANLLGMLPGAGQMKEQLKQIDDRDLDRTAAIIR"
     gene            complement(1212..2102)
                     /locus_tag="VFQ79_08915"
     CDS             complement(1212..2102)
                     /locus_tag="VFQ79_08915"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0139818.1"
                     /translation="MAGDVPVIDAHMHLFDTGRPDGVPWPDKEDIILYRPALPDRYCH
                     IAEPLGIRGAIVIEASPLLEDNDWLLDLAADCTIIVAVVGNIEPGTPEFGSHLERFVR
                     NPVFRGIRFGTLWGRSLTQALSTRQVIADLKLLASAELELDVIGNDWTLLGDVVRLTD
                     CVPDLRVVINHLPEIDPPTEPRFLDVYRANLQKLAQRPQVYAKLSALLRRQACVFFSW
                     EGSMGKAVAFVAAVVAALILVGWVVSSLIGPLFYLLLGALVVGGGIYLYARAKKSLAP
                     GTRTQRRIEAAAKTYRMRNR"
     gene            complement(2437..2513)
                     /locus_tag="VFQ79_08920"
     tRNA            complement(2437..2513)
                     /locus_tag="VFQ79_08920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2477..2479),aa:Arg,seq:tct)
     gene            2682..3857
                     /locus_tag="VFQ79_08925"
     CDS             2682..3857
                     /locus_tag="VFQ79_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139819.1"
                     /translation="MLRAALISNDNEVLEHLENLSRELGQFQIGRKFHRQPTESELIQ
                     CVRAYGPEVVFLSLEYVAAAGDIHALLEKASPGVQLVAVGRYGDPQILLQLMRMGVRE
                     FLTLPVNLNEFEQMMARIRAILEKQPPTARSSDLLYSFLPAKAGCGTSTIALNTSMAI
                     AEKQSTSVLLMDFDLNSGMIRFMLKLENPYSLLDAVEHCDALEEELWAQLVTHRGRLD
                     LLHSGGLNPHIRIDPAQVRKLSDYARRNYQVICADLSGNLERYSVEMMKQSKRIFLVS
                     TPEIASLHLAREKLAYLKSIELSDRVSLIVNRFHKRALISKEEIEDLMSLPVTMTLPN
                     DYQRVHRSLTSATEIERGSELGKQFSKLADVILERKQQPNTVTKKRFVEYFSTAPAR"
     gene            complement(3886..4137)
                     /locus_tag="VFQ79_08930"
     CDS             complement(3886..4137)
                     /locus_tag="VFQ79_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139820.1"
                     /translation="MPAPFTFGNASPSYNDVRNFRVFTEDFSAVKSTRIREATELEFT
                     ATFINAFNRHRFTDFTATRNTATFGQATGSSLGRIITLG"
     gene            4171..4302
                     /locus_tag="VFQ79_08935"
     CDS             4171..4302
                     /locus_tag="VFQ79_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139821.1"
                     /translation="MESKSPSGMPVERSTPLTTLGRSVRLKIGSVFDVLMLSGVPDW"
     gene            complement(4576..5727)
                     /locus_tag="VFQ79_08940"
     CDS             complement(4576..5727)
                     /locus_tag="VFQ79_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0139822.1"
                     /translation="MGDAFPSFTFGDSYSPLGRNSLSEGLEGSTVARNMLNWTRGKQL
                     LKFGVEYRRIRSRSKDWSTSAGNYTFSNQGTALPTSLSNTGDSYASFLLGQTATASMS
                     LPVQSESTRPYWGFFVQDDYRVTSRLTLNLGLRYEFTGAPHEHNDQYSLVDLNVPNPA
                     AGGRLGASVFAGKGEGHIGSSYLYNTDYSGIGPRFGFAWQVAANTVVRGGYGLFYGGN
                     DLDIVTTGYRTVASHATLDQGVTPAFILEEGFPGSYSPNPVLTPAMLNGQNVTARSSS
                     APRMPRTQNWSLSVQRELNASWVAELSYVANKATRLSSPSMINDNQVDPRYLPLGSLL
                     TQQVTSAAAVAAGIVKPYPDFTGSVAQALRPYPQYLTVTEQAAKAGSSF"
     gene            complement(5666..7147)
                     /locus_tag="VFQ79_08945"
     CDS             complement(5666..7147)
                     /locus_tag="VFQ79_08945"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0139823.1"
                     /translation="MHTSISSVHRGILLPALAAVLFAGSLWGQSAELSGRITDPSGEI
                     INGAKVTLTQQATMLQTGTQSNESGYYFFPRGQPGVYRLVISHQGFKAVERSGINMAP
                     ADRRRVDVQLELGSVAETINVMADASQLQADSAEVSTTVTSREFAQLPQIQYNRMRSP
                     ANFTYLSPGVHGNVATNGRETVAASNNIRINDSRSTSNEIYMDGMPGRTNFNETAPPV
                     DAIGEFKLQANQLSAEYGNTGSAVITFSVKSGTNNFHGLLSNIFRNEKLDARSFLSPN
                     RSTIRQNEFGGTFGGPVTIPKLYDGKNRTFFFFSYTGSRKRGLDQTQRLRVPSLAERE
                     GDYSANPRLIYDPATTRSNGTVYVRDPFAGNWIPASRFDAAAAKVTSLLPSPNLSGAG
                     LLNYTDSIGERLLDPNVYLVRLDHAFSEKHRVFCTYNHTYIPRQNQTAAMADPWSDRT
                     LQNISSHMVRANWLGRRAGPEERGGRRFPLLHLRRLLFSAGPQ"
     gene            complement(7224..8252)
                     /locus_tag="VFQ79_08950"
     CDS             complement(7224..8252)
                     /locus_tag="VFQ79_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0139824.1"
                     /translation="MKAVRIHQHGGPEVLVQEDIPEPQIRPDEILLRVRAVSLNHLDL
                     FVRAGIPGQKFAMPHILGSDIAGEVLETGALTGRIKPGQRVLLSPGLSCRQCERCVSG
                     NDNRCARYTLFGSGIPGGYRELMAVPEYAAIPIPDDLSFEDAAAVPLVFVTAWHMLFS
                     RARLEAGEDVLVLAASSGVGSAAVQIAKLFHCRVIATAGSDDKRAKAAELGADLTIDH
                     YRQDISAEVKKFTGRRGVDVVFEHVGAATWPKSMESLAAGGRVVTCGATTGFDVKVDL
                     RYLFFKQQSVLGSFMGTLGDLHRVLRFVFRKQLKPVVDKVFPLSAVREAHERLENKEQ
                     FGKIVLVP"
     gene            complement(8270..9934)
                     /locus_tag="VFQ79_08955"
     CDS             complement(8270..9934)
                     /locus_tag="VFQ79_08955"
                     /inference="COORDINATES: protein motif:HMM:NF011305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEU0139825.1"
                     /translation="MPRSSPLLPLVEIRLEDYLRFLDNFTAFCVAPLVVWIFVSGLDD
                     LLIALAFLYSWLRKLRPPVTDSQLRSEPEKRIAILVALWQEHEVIGSMIRHNVASLEY
                     SNYDFFLCTYPNDEETVAAVAELEDSFPNVHLAICPHDGPTSKSDCLNWVYQNLLLFE
                     QQRQVRFEILVIHDAEDVIHPAALKLINYYSRKWEMIQVPVLPLRTSLFELTHGVYCD
                     EFAEYQIKDIPARQTLGGFIPSNGVGTGFRRDTIEKLASCSSNRVFEPECLTEDYEIG
                     LRLHELGCRQLFVPVQFQGPEPMATREFFPRTFRSALQQRTRWITGIALQSWERHGWR
                     GGVRRAFWFWRDRKGVVGNPLSAFANLLLLYVLLTWAAGHTPAVPRSEAIPPFAMLLL
                     PWTLFLQIFQLVFRTACATRIYGPAFAAGVLVRSTWANVINSIATVLALHRYARAKWR
                     HEPLRWLKTEHTYPSLASLTQALAGFMPIDIDEVPSGVLRALPAHLVEKWHVLPFKVA
                     AGALHLAGPHPPPEGFQADIRRFTNLRIRFHLVDELDFEELKQRLG"
     gene            10043..10534
                     /locus_tag="VFQ79_08960"
     CDS             10043..10534
                     /locus_tag="VFQ79_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139826.1"
                     /translation="MQFWPVLFLAAAATAFGASPSRERIDDSAALVNQLQAEEWLRVW
                     QKRLSLDDWKVELRIVRSWELKPDTLGNLRWNSTARTATIRVLDPVDYDLPLAKIPRD
                     IEMTIVHELLHLQLSVLPKDSSKKADEERVVTRIAEALMNLDNERRPAPATLSRMGAE
                     AGQ"
     gene            complement(10528..10755)
                     /locus_tag="VFQ79_08965"
     CDS             complement(10528..10755)
                     /locus_tag="VFQ79_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139827.1"
                     /translation="MDPQKAIGRPPEDLSLTERTAFAGKTIALEIYSPQTLPVRRIEA
                     IGDSPEECARALMERGLDPRKFELISLAPPY"
     gene            complement(10792..11928)
                     /locus_tag="VFQ79_08970"
     CDS             complement(10792..11928)
                     /locus_tag="VFQ79_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139828.1"
                     /translation="MSTRNRLAAVLFAGALAAWPQSEPLDPRSTLHITLPEDSPISVV
                     SADWGESRATARGGAMVLDLRTSLSLRNSGQAKIRGVTLLVEAQDVTPGGKASVAVPS
                     LDAGEGDVFPVRVDMRLMRPLQSGTGPLVTIKLDGVLFDDLSFYGPNELNSRRSMRAW
                     EMEARRDRQYFKTVLDSGGPEGLRQAFLSSVAALSSRPRMDVRFARSGRATNFEAEHE
                     LQFAFLRFPDSPVDPISGVARASGSEIRAPRIDVRNRSKRAVSYVEIGWILKDRNGRQ
                     FLAGSVPSSDGLGLAPGQTGKILESTSLRFSSSSGRIVDIQGVTGYVSQVEFDNGEIW
                     IPSRSSLNQSGLQGVAAPSPEEQRLTNIYVKKGLEAVIQELRKF"
     gene            complement(11912..12484)
                     /locus_tag="VFQ79_08975"
     CDS             complement(11912..12484)
                     /locus_tag="VFQ79_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139829.1"
                     /translation="MTHPALAELALFAGGDHGVFSGWRIRLHLNRCERCREEVRAFRE
                     AQEKAVNLAEALPEGLNWQRLAGEMTANIRVGLAAAECVPVRPAPERRLGWRPVTALA
                     SATMLVATGWWLTMPRPNLDTAGFEARPAPEAVVNASSGGIELNRNGRTLTLLNSGEA
                     VVLVNAQGTARSRYVDSETGQVTINHVYTE"
     gene            complement(12481..13143)
                     /locus_tag="VFQ79_08980"
     CDS             complement(12481..13143)
                     /locus_tag="VFQ79_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0139830.1"
                     /translation="MVRLLHTPATEHSQSGPPAPVRGACGPAGAIEGLNRMMREAARP
                     PLESSPDESQQRRDRFVQEHMRRIFLQIYRMVGNIDDAQDLTQEAFIKALQREDQLKD
                     LDKAAHWLSRIASNTAIDFLRRHGRISFCELDDAPEPRSTPPYESPEDILLRSEQRAY
                     LDHGLRLLSGRERTALLLRDVEGLPAEEVARQLNCSKATVRSHIANARIKFRRYIERR
                     KP"
     gene            13215..14372
                     /locus_tag="VFQ79_08985"
     CDS             13215..14372
                     /locus_tag="VFQ79_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEU0139831.1"
                     /translation="MRDVAIIGIGKTPFGAFPDRDLRSLATEAGSKALTDANVKPAEV
                     EAFYLGNFAGPSFIGQSHLAPYIAAPLGLTGIPAVRCEAACASSGAAFFQAVSMVGAG
                     LYDLVLVIGVEKMTSQPTPRVTEILASAGDLEGEVKAGATFPALFAMIARRHIYQYGT
                     TREQMSLVAVKNHANGARNPYAHMRKPVTLEQAMAGRLVAEPLNLYDCSLVSDGAAAV
                     VVAPLERADEFNGKPVRVRGIAQASDFVALDSKDDITRFRATQKAADKAYRMADATAR
                     DIQFAEVHDCFTIAEIVALEDLGFVAKGEGGPYTQDGCTALNGPRPVNASGGLKSKGH
                     PVGATGAGQLCDLVLQIRGEAGENQVERHSLGLAQNLGGSGATSVVTILSV"
     gene            14369..14596
                     /locus_tag="VFQ79_08990"
     CDS             14369..14596
                     /locus_tag="VFQ79_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEU0139832.1"
                     /translation="MSDRPPLTAGTIYTETVVHSAPEALVNEAPYQIAIVEFDSGSRV
                     TGRIEGDRVSIGDRVSFSGFRNEVPFFRKSS"
     gene            14593..15756
                     /locus_tag="VFQ79_08995"
     CDS             14593..15756
                     /locus_tag="VFQ79_08995"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0139833.1"
                     /translation="MKLAERMSRLGTETAFEVLVRARALEAQGRSIIHLEIGEPDFET
                     PRHIREAAKKALDEGWTHYGPTQGYPELREAIASSICHSRGIPVDPSHICVVPGGKPI
                     LFFPMLALLEPGDEVIYPNPGFPIYESMIRFLDAVPVPMPLVESLGFSFDLDLFRQRL
                     SNKTKLVILNSPQNPTGGVIPAPDLAAMAEMLRDRDVMVLSDEIYSRIYYDEPPVSIT
                     RFPGMLEKTILLDGFSKIYAMTGWRMGYGVMPPWLVEAVNKLMVNSNSCTASFTQRAG
                     LAALNGPQGESEKMVAEFRRRRDAFCQGLNSLPGFRCAIPKGAFYAFPNIEGTGMRSR
                     ELADALLAETGVAALSGTSFGEYGEGYLRFSYANSLENLMEAVDRIRKFLNNR"
     gene            15757..16482
                     /locus_tag="VFQ79_09000"
     CDS             15757..16482
                     /locus_tag="VFQ79_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU0139834.1"
                     /translation="MEVSFQLKMGQRTMDAGVNLPDVPVRTLDLLPVLLPFASAIVDA
                     STADVEEEGKHISCRAGCGACCRQLVPISRPEALYLAAHLDTLPPERRTIIRERFQVA
                     VERLRETGMRERLTGFEDQGQDARREIGMDYFRLGIACPFLEEESCGIYEHRPMACRE
                     YLVTSPAANCADPHPETIDTVPLYARPSVILYRFGDGKGEDRPRFLPLVFALEWAAAH
                     AADTQAELPAPEMFRNFVRKVAE"
     gene            complement(16498..17460)
                     /locus_tag="VFQ79_09005"
     CDS             complement(16498..17460)
                     /locus_tag="VFQ79_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HEU0139835.1"
                     /translation="MKEIRAGAGQFEVRDGDKAYNLKRIAELSGKAAGEGAQLVSFNE
                     CSISGYTFLEKLSREELFALAECVPGGPSTEKLAEISRSLGVALAAGLVEREGDRMYN
                     CYVVVDENGFVAKHRKIHAFISQYLTCGDRYTVFDLLGCRFGILTCYDNNLPENVRLT
                     AMMGAEIILMPHVTGCLPSPMPGRGTVDPELWHNRERDPVRLRQEFDGPKGRGWIMRW
                     LPARAWENGVFAIYSNPIGVEGGTIKPGGSMILDPFGEVLVECRTLGDDVVVATLDPQ
                     KRELASGPSYIRARRPELYGMMTELNPHVGPDGRPEVWWQKHRK"
     gene            complement(17477..17920)
                     /locus_tag="VFQ79_09010"
     CDS             complement(17477..17920)
                     /locus_tag="VFQ79_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0139836.1"
                     /translation="MVETVCSILRRKGGEVWTISPESTVYDALLMLADRDIGALLVAE
                     SGRLVGIISERDYARKVILKDKRSKDTTVHEIMATDLVTVTPDHTVDECMRIMTRHRI
                     RHLPVLEEGKLAGLVSIGDLVNSIISAQAETIDQLSNYIKGKYPA"
     gene            18031..>18370
                     /locus_tag="VFQ79_09015"
     CDS             18031..>18370
                     /locus_tag="VFQ79_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0139837.1"
                     /translation="MHSRRSFTGALLGFSASQSLPAAAADPAWMGIAEGGGLLRRKKL
                     SPIEWTKACLDRIEKLNPRLNAFITVTAEQALADARRMESELQAGKVRGPLHGVPIAL
                     KDLIDTTGIRT"
BASE COUNT         3565 a         5544 c         5690 g         3571 t
ORIGIN      
        1 ccggatgatc gccgccgtgc ggtcgaggtc gcggtcgtcg atctgcttga gctgctcctt
       61 catctgcccg gcgccgggca gcatgccgag caggttggcg atcgggccca tcttgcggat
      121 cgccatcatc tgctcgagga agtcctcgag agtgaagccc tcgcgggagg cgagcttgcc
      181 cgccatccgc tcggcctgct cctcgtcgaa ggcctgctcg gcctgctcga tgagggtgag
      241 cacgtcgccc atgccgagga tgcgcgaagc catccgctcg gggtggaaga cgtcgaagtc
      301 ggccagcttc tcgccggtgg acgcgaacat gatcggctgc ccggtcacgt agcggaccga
      361 gagcgcggcg ccaccgcgcg cgtcgccgtc gagcttggtg aggacgacgc cggagaagcc
      421 gacaccctcc tggaaggccg tcgcggtggt gaccgcgtcc tggccgatca tggcgtcgac
      481 cacgaacagc gtctcgtcgg gccggacggc gtcgcggatc gcggcggcct gggccatcag
      541 ctcggcgtcg atgccgaggc ggccggcggt gtcgacgacg acgacgtcgt gcatggtgcg
      601 ccgggcgtgc tcgatcgact cccgcgcgac ctcgaccggg tcgccgacac cgttgccggg
      661 ctggggcgcg aagacgtcga cgccggcccg gccggcgagc acctggagct ggttgaccgc
      721 gttggggcgc tgcaggtcgg cggcgaccag caggggttgg tggccctggc ccttgagcca
      781 gcgggcgagc ttgccggcca gcgtcgtctt gccggaaccc tgcagaccgg ccagcatgat
      841 cacggtcggc ggctgcttgg cgaactgcag gcgccggccc tcgccgccga ggacgttgat
      901 cagttcctcg tgaacgatct tgatgatctg ctgggccggg ttgagcgcct gggagacctc
      961 ggcgccgcgc gcgcgttcct tgaggttgaa gatgaagctc ttgaccacgg gcagcgcgac
     1021 gtccgcctcg agcagcgcga gacggatctc gcgtgcggtg gcgtcgatgt cggcatcggt
     1081 gagccggccc ttgccgcgga gcttggtgaa gatcccggac aggcggtcac tcaaagtgtc
     1141 aaacacggtg caacgtcccg ttgtcagaag gtggtggcga tgccgaccag cctagccctc
     1201 cgggtgaaag ctcaccggtt gcgcatgcgg taggtcttcg ccgcggcctc gatgcgccgc
     1261 tgggtgcggg tgccgggggc cagcgacttc ttcgccctgg cgtagaggta gatcccgccg
     1321 ccgaccacga gcgcgcccag gagcaggtag aacagcgggc cgatcagcga gctgaccacc
     1381 cacccgacca ggatcagtgc ggcgaccacg gccgccacga aagccactgc cttgcccatc
     1441 gagccctccc agctgaaaaa aacacacgcc tgtcggcgga gaagtgccga aagcttcgca
     1501 tagacctgtg gacgctgcgc caacttctgc aaatttgccc gataaacgtc caggaacctg
     1561 ggttcagttg gcggatcgat ctccggcaag tgattgatca caacccgcag gtcgggaacg
     1621 caatctgtaa ggcgcaccac gtctccgagc agagtccaat cattacctat gacatccaat
     1681 tcgagctcgg cacttgcgag aagcttaagg tctgcgatta cctggcgggt agacagcgcc
     1741 tgagtcaggc tccggcccca cagagttcca aaccggatac ctcgaaacac cgggttccgg
     1801 acaaaccgtt ccaggtggct gccaaactcc ggtgtgccgg gttcaatatt gccgacgacc
     1861 gcgacgatga tcgtgcaatc ggctgccaaa tccagaagcc agtcattgtc ctccagcaag
     1921 gggcttgcct cgatgacgat ggcgccccga atacccaggg gctctgcgat gtgacagtac
     1981 cggtccggca gcgcgggtcg atacagaatt atgtcttctt tatccggcca aggcacaccg
     2041 tcgggccgtc cggtgtcaaa cagatgcatg tgcgcgtcaa taaccgggac gtcgcctgcc
     2101 atttatcgct cgtccttcac tttgctgcgg cagattcctc gcgtcggact ctgcaacttt
     2161 attggggttc cgaatcggcc ccacggcacc ggttgctcca ggtagatctg tgtgcttctt
     2221 ccgtttcata ccacccaccc aagtcggtgc ggattgttcc cggcataata gcatttacgc
     2281 gcacatgata ctttgccggt tccagtgcca tggcacgagt tagcatctga attcctcctt
     2341 tcgagacgca gtaagcagag gaaccgggat ccgctatctc tgcgtcatgc aggaaatgag
     2401 cgaagcttag tacccgcaag ttgttgattc tgaaagtggt aggcgcggca ggactcgaac
     2461 ctgcgaccct ctgcttagaa ggcagatgct ctatccacct gagctacgcg ccctttgcct
     2521 ggactacttg attttactat gtggggcgct cctcgatccg gcttcgaagc tgacatccct
     2581 tttggctcgt ctaaggtttt ctgccctggc caactaaggt tgacccgcaa ttgaagcgtt
     2641 gctgtttcca ccttactttg gaatttggaa gaggaggacg ggtgttacgc gccgctttga
     2701 tttcgaatga caacgaggtg ttagagcacc tcgaaaacct gtcgcgggaa ctcggacaat
     2761 ttcagatcgg gcgcaagttt catcgccagc ccacggagtc tgaactcatt cagtgcgtcc
     2821 gggcctacgg gccggaagtc gtatttctca gcctggaata cgtcgcggca gcgggcgata
     2881 ttcacgccct gctggaaaaa gcatcacccg gagttcagtt ggtggcggta gggcgttacg
     2941 gcgatcccca aattctgctc caactcatgc gcatgggggt gagagaattc ctgacgctgc
     3001 cggtcaacct gaacgagttc gaacagatga tggctcgaat tcgcgcgatt ctcgaaaagc
     3061 agcctcccac agcccggtct tccgatctgc tgtattcttt tcttccggcc aaagcggggt
     3121 gcggcacatc cacgatcgca ctgaatacca gcatggccat agcggaaaag cagagcacgt
     3181 ccgtcctgtt gatggatttc gacctgaatt caggcatgat ccggttcatg ctgaagctgg
     3241 agaaccccta ttcgcttctc gacgccgtag aacactgcga cgccctggaa gaggagttat
     3301 gggcgcaact cgtcacccac cggggacggc tggatctgct ccattcgggc gggctgaatc
     3361 cccatatccg catcgacccg gcccaggtaa ggaagctcag cgattacgca cgccgcaact
     3421 atcaggtgat ctgcgcggac ctctcgggca atctcgaacg gtattccgtc gagatgatga
     3481 agcaatcgaa gcgcattttc ctggtctcga cgcctgagat cgcttccctg catctggcgc
     3541 gagagaagct ggcttatctc aaaagcatcg agttgagcga ccgggtgagt ctgatcgtca
     3601 atcgctttca caagagagct ttgatttcga aagaagagat cgaagatctg atgagcttgc
     3661 cggtcaccat gacacttcca aacgattacc agcgtgtaca tcgctcgctc acctccgcga
     3721 ccgaaatcga acgcggctcg gaattaggca agcagttcag taaattagcc gatgtcatcc
     3781 tcgaaagaaa acaacagccg aacacggtga cgaaaaagcg gtttgtagag tacttctcta
     3841 ccgccccggc gcgctaaagc gggatggctg cctagaattt cagcttcagc ccagtgtaat
     3901 gatgcggccc aggctcgaac ctgtggcttg gccaaaggtc gcggtgttgc gcgtggcggt
     3961 gaaatcggtg aaccggtgcc ggttgaaggc gttgatgaac gtcgccgtga actccagctc
     4021 ggtagcttcc cggatgcgcg tgcttttgac ggccgaaaaa tcctcggtga agacgcggaa
     4081 gttgcgcacg tcgttgtagg aggggctggc gttgccaaag gtgaagggcg ccggcacggc
     4141 aaaggcgtcg gcgttgatgc ggcggctggt gtggagtcga aatcgcccag cgggatgccg
     4201 gtggagcgat caacgccact caccacgttg ggacgcagcg tgcggttgaa aatcggcagc
     4261 gtgttcgatg tgctgatgct gagcggtgtc ccggattggt agcggtgaat gccgttgatg
     4321 ttccagcccg aaaccagggc cgagagcacg ccgccgcggt tcagccacgg cttgcccgca
     4381 ccaaaaggga gatcgtaggt gtattggact accaacgcgt ggggaatgtc gtagttcacc
     4441 agagtccact cggcgccgcg gttgaaatga tcctggacgg tgtcggaagt gcccagcagc
     4501 cacgaagacg tacagtgcgc atctagcgtg acaccggccg agtagcgctt gcggacgcgg
     4561 gccacaaagc tctggttaga acgacgagcc cgccttggcg gcctgctcgg tgacagtcag
     4621 gtactgcgga taagggcgca gcgcctgcgc cactgagccg gtgaagtccg gatacggttt
     4681 gacaataccg gcggcgacgg cggcggccga ggttacctgc tgcgtcagca gcgagcccag
     4741 cgggaggtag cggggatcca cctggttatc gttgatcatg gagggcgacg acaggcgggt
     4801 ggccttgttg gccacataac tcaactcggc gacccaggaa gcgttgagtt cccgctgcac
     4861 gctgaggctc cagttctgcg tgcgaggcat gcgcggcgcc gaggaggagc gcgcggtgac
     4921 gttctggccg ttgagcatag cgggcgtgag cacggggttg ggcgagtaac tgcccgggaa
     4981 gccctcctcg aggataaagg ccggagtcac gccctggtcg agggtggcat gactggctac
     5041 ggtgcggtaa ccggtggtga cgatgtccag atcgttgccg ccataaaaca ggccatagcc
     5101 gccacggacc accgtgttcg cggctacctg ccaggcgaag ccgaagcgcg ggccgattcc
     5161 ggaatagtcg gtgttgtaca ggtagctcga gccgatgtgg ccctccccct tgccggcgaa
     5221 gaccgaggcg cccagacgcc cgccggcggc aggattcgga acgttgagat ccaccaggct
     5281 gtactggtca ttatgctcgt gcggcgcacc ggtgaactcg tagcgcaatc ccaggttgag
     5341 cgtcaggcgg ctggtgacgc gataatcgtc ctgcacgaag aagccccagt agggccgcgt
     5401 agattcggac tgaacaggaa ggctcatgga agcggtggcc gtctggccga gcaggaagct
     5461 ggcgtaggaa tcgccggtgt tgctgaggct ggtgggaaga gcggtgccct ggttggagaa
     5521 ggtgtaatta cccgccgaag tgctccagtc cttcgagcgg ctgcggatcc ggcggtactc
     5581 gactccaaat ttgagcagtt gtttgccacg cgtccagttg agcatgttcc tcgcgacggt
     5641 ggatccttcc aggccttccg agaggctatt gcggcccagc ggagaatagg agtcgccgaa
     5701 ggtgaaggag gggaaagcgt cgcccaccac gttcttcagg cccagctcgg cggcccaacc
     5761 agttggcccg caccatgtgg ctggagatgt tctggagcgt tcggtcgctc cagggatcgg
     5821 ccatggcggc ggtctggttc tgacgcggga tgtaggtgtg gttgtaggtg cagaagacgc
     5881 ggtgcttctc gctgaaggcg tgatccagcc gaaccaggta gacgttggga tcgagcagcc
     5941 tctcgccgat cgagtctgta tagttgagca acccggcgcc gctcaagttg ggagagggca
     6001 gcaggctggt gacttttgcc gccgccgcgt cgaagcgcga agcgggaatc cagtttccag
     6061 cgaagggatc gcgcacgtag acggtgccgt tagaccgcgt agtggcggga tcgtagatca
     6121 ggcgcgggtt ggcgctgtag tcgccctcgc gctcggccag ggaggggacg cgcaggcgct
     6181 gggtctggtc gaggccgcgt ttgcgcgaac cggtataaga gaaaaagaag aaggtgcggt
     6241 ttttcccgtc gtacagcttc gggatggtca ccgggccgcc gaacgtaccg ccgaattcgt
     6301 tctggcggat ggtggagcga ttgggcgaaa gaaagctgcg cgcgtccagc ttctcgttac
     6361 ggaagatgtt ggacagcagg ccgtggaagt tgttggtgcc ggacttgacg ctgaaggtga
     6421 taaccgccga gccggtgttg ccgtattcgg ccgagagctg gttggcctgg agcttgaact
     6481 cgccgatagc gtctacgggc ggcgcggtct cgttgaagtt ggtgcggccg ggcatgccgt
     6541 ccatgtagat ttcattcgaa gtcgagcggc tgtcgttgat acggatgttg ttgctcgccg
     6601 ccaccgtctc gcgtccgttg gtggcgacgt tgccgtgtac gcccgggctg agatacgtga
     6661 agttggccgg gctgcgcatg cggttgtact ggatctgcgg caattgggcg aactcgcgcg
     6721 aggtcactgt ggtcgaaacc tccgccgagt ccgcctggag ctgcgaagcg tcggccatga
     6781 cgttaatagt ctcggccacc gagccgagct cgagctggac gtccacgcgg cgccggtctg
     6841 cgggggccat attgatgccg gagcgctcca ccgctttgaa accctggtga gaaatcacca
     6901 gccggtaaac gcccggctgc ccgcggggga aaaaataata accggactcg ttgctctgcg
     6961 tcccggtctg tagcatcgtg gcctgctgcg tcaacgtgac cttggcccca ttgataatct
     7021 cacccgaagg atcggtgata cggccgctga gctcggcgct ctggccccac aagctgccgg
     7081 cgaaaagaac cgctgccagc gccggaagaa gaattccgcg gtgcacggag gaaatagaag
     7141 tatgcatgtg gtctccctgg caaagaaaca cactgtagca ccccgccgtg acaggggaat
     7201 tacggcgggg taacaactag ggctcaaggc acaaggacga ttttgccgaa ctgctccttg
     7261 ttttccagcc gttcatgcgc ctcgcgaacc gcggacagag gaaacacctt gtcgacaacg
     7321 ggttttaatt gtttccggaa aacaaaccgg agcacccgat gcaagtcgcc gagggtgccc
     7381 atgaaagagc cgagcacgct ctgctgtttg aaaaagagat accgcagatc gaccttcaca
     7441 tcgaaccccg tggtggcccc acacgtcacg acgcggcctc cggcggccag gctttccatg
     7501 ctcttgggcc acgtcgccgc gcccacgtgt tcgaatacca cgtcgacccc gcgccggccc
     7561 gtgaactttt tcacttcggc ggagatgtcc tggcggtagt ggtcaatcgt gagatctgcg
     7621 ccgagctcag cagcctttgc ccttttgtca tcgcttccgg cggttgcgat cacccggcag
     7681 tggaagagct tggcgatctg aaccgcagcc gatcccacgc cgctggatgc agccagaacc
     7741 aggacatctt ccccggcctc caagcgtgcg cggctgaata gcatatgcca cgccgtgaca
     7801 aaaaccagcg ggaccgctgc cgcgtcctca aagctgagat cgtccggaat gggtatggct
     7861 gcgtactccg gcacggccat aagttcccgg tatccccccg gaataccaga accgaaaagc
     7921 gtgtaccgcg cgcagcgatt gtcgttgccc gaaacacatc gctcacactg gcggcagctc
     7981 aatcccggtg agagcagaac gcgctggccg ggcttgattc ttccggtgag tgcgccggtc
     8041 tccagcactt cgccggcgat gtcgctgcct agaatgtgcg gcatcgcgaa cttctgcccg
     8101 ggaatgccgg cgcgcacaaa caggtcgagg tgattcagcg aaaccgcgcg tacccgcaac
     8161 aggatttcat cgggccggat ctgcggctcc gggatgtctt cctgaacgag aacttccggc
     8221 ccgccgtgtt ggtggatgcg aacggccttc atagtgcgtt ctcgtcagtt taccccaggc
     8281 gttgcttcaa ctcctcgaag tccagctcgt caacaagatg gaaccggatg cgcaggttgg
     8341 tgaaccgccg gatatcggcc tgaaagcctt ccggcggcgg atgggggccg gcgagatgca
     8401 gcgctccagc agcgaccttg aagggaagca cgtgccattt ttcgaccagg tgcgcaggca
     8461 gagcccgtag cactccggac ggaacctcgt cgatatcgat cggcataaag cctgccagcg
     8521 cctgagtaag cgatgccagg gagggatagg tatgctcggt tttgagccac cgaagaggct
     8581 cgtgtctcca cttcgcgcgc gcataccggt ggagagccag aacggttgcg atggagttga
     8641 tgacgttggc ccatgtgcta cgaaccagga caccagccgc gaaggcgggt ccatagattc
     8701 gcgtcgcgca cgcggtacgg aacacaagtt ggaatatctg gagaaacaaa gtccacggca
     8761 gcagcagcat tgcgaaaggc ggtattgcct cggacctggg aaccgcgggc gtatgtcccg
     8821 cggcccaggt tagaagcaca tacaacaaca gcaggttggc aaatgcactg aggggatttc
     8881 ccacgacacc tttacgatcc cgccagaacc agaaggcgcg gcggaccccg ccccgccacc
     8941 catggcgctc ccagctttgt agcgcgattc cagtaatcca gcgggtacgc tgctgtaagg
     9001 cggagcggaa tgtgcgcggg aagaattctc tggtggccat cggctccggt ccctggaatt
     9061 gaaccggaac gaacaactgg cggcagccga gttcatggag cctgagcccg atttcatagt
     9121 cttcggtcaa gcattcgggt tcgaagaccc ggttgctgga acacgatgcg agcttttcga
     9181 ttgtgtcgcg gcggaacccc gtgcccacac cgttcgacgg tataaatccg cccagggtct
     9241 ggcgggccgg gatgtctttg atctggtatt cggcaaattc gtcgcagtac acgccgtggg
     9301 tcaactcgaa taaagaggtc ctcaacggca gcaccggcac ctgaatcatt tcccatttgc
     9361 gggaataata atttatcaac ttcagagcgg cggggtggat gacatcttcc gcgtcgtgaa
     9421 tgacgagaat ttcgaagcgt acctgccgct gctgctcgaa gaggagcaga ttctgataga
     9481 cccagttcag acaatccgat ttcgacgtcg ggccgtcgtg cgggcagatt gccagatgga
     9541 cattcgggaa actgtcctcg agctccgcca cggccgccac agtttcttcg tcgttcggat
     9601 aggtgcagag aaagaagtcg taattggaat attcgaggga ggcgacgttg tgccggatca
     9661 tgctgccaat cacttcgtgc tcctgccaga gcgcaaccag aatggcaatc ctcttttcgg
     9721 gttcggaccg aagttgagaa tccgtcaccg gcgggcgcag tttacgcagc caggagtaaa
     9781 gaaaagccag agcaatcaac agatcatcga gcccgctaac gaagatccag acaaccagcg
     9841 gcgcgacaca aaacgccgtg aaattatcga ggaatctcag atagtcttcc agacggattt
     9901 ccaccagggg aagtagtgga ctactcctcg gcaatctctc aaatcagtgg cttcccccca
     9961 aatgaggttg actttcccct cgaagtacct ggtacttccc atacttaaat ctgagcattt
    10021 gaggtaagat tatgttttta aaatgcaatt ctggccggtt ctttttctgg ctgcggctgc
    10081 gacagccttc ggagcttctc cctcccggga gagaatcgac gattccgctg ctctggtgaa
    10141 tcaattacag gctgaagagt ggcttcgcgt ctggcagaag cggctcagcc tggatgattg
    10201 gaaggttgaa ctccggatcg tccgaagttg ggagttgaag cccgacactc tcggaaacct
    10261 gcgctggaac agcacagccc ggacggccac catccgcgtg ctcgatcccg tcgattacga
    10321 tctgcctctg gcgaaaatac cccgcgatat cgaaatgacg atcgtccatg agctattgca
    10381 tcttcagctt tcggtcctgc cgaaagacag ctcgaagaag gcggacgagg agcgcgtggt
    10441 gacgcgcatt gcagaagcct tgatgaacct ggataacgaa cgccggccgg cgccggcaac
    10501 gctatcccgc atgggggccg aggccggtca ataaggcggg gccagcgaga tcagctcgaa
    10561 ttttctcgga tcgagcccgc gctccatcaa cgcacgcgca cattcttccg gagagtcccc
    10621 aatcgcctcg atgcggcgga ccggcagggt ttgcggtgaa tagatctcca gggcaatggt
    10681 ttttcccgca aatgcggtgc gctccgtcag cgaaaggtct tcgggcgggc gtccgatggc
    10741 tttttgagga tccatgtcag gtggagccgc agggtgcggc taaaccctat tctaaaactt
    10801 ccgaagctcc tggatgacgg cttccagccc cttctttacg tagatattcg tcaaccgctg
    10861 ttcttccgga gaaggagccg ctacaccctg caatccgctc tggttcaaag aactgcggct
    10921 gggaatccag atctctccgt tatcgaactc cacctggctg acgtagccgg tgacgccctg
    10981 aatatccacg atgcggccgg acgaggacga aaaccggagc gaagtcgact ccagaatctt
    11041 acccgtctgt ccgggagcga ggcccagacc gtctgatgag ggcaccgaac cggcgaggaa
    11101 ttgcctgccg ttgcggtcct tcaggatcca tccaatttcc acataggaga cggcgcgctt
    11161 cgacctgttt ctcacatcga tgcgcggcgc ccggatctcg ctgccggagg cgcgcgctac
    11221 accggagatg gggtccaccg gcgagtccgg aaaacgcagg aaggcgaact gcaactcgtg
    11281 ttccgcctcg aagttcgtag cgcggccgct gcgtgcgaac cgcacatcca tccgcggccg
    11341 tgaggatagc gcggcgacgc tggagagaaa cgcctgccgc aggccctccg ggccgccaga
    11401 gtcgagaacg gttttgaagt actgccggtc ccggcgggct tccatttccc acgctctcat
    11461 cgaccgccgc gagttcagct cattcggccc gtaaaagctc agatcgtcga acaatacacc
    11521 gtcgagcttg atggtgacca gcggccccgt gcccgattgc aacggccgca tcaggcgcat
    11581 gtccacgcgc accggaaaaa catccccctc tccggcatcc aggcttggca cagcgacaga
    11641 ggctttgccc cccggagtca cgtcttgcgc ctccaccagc agggtcacac ctcggatttt
    11701 cgcctggccc gaattgcgca gcgacaggga tgtgcgaaga tccagcacca tggctccgcc
    11761 ccgcgcggta gcccgcgatt caccccaatc ggctgacacc acgctgatgg gcgagtcttc
    11821 cggtagcgtg atgtgcaaag tactgcgggg gtccagcggt tcgctctgcg gccacgccgc
    11881 gagcgccccg gcgaacagca ccgctgccag cctattccgt gtagacatgg ttaatcgtca
    11941 cctgacccgt ttccgagtcg acgtaacggg accgggccgt tccctgcgca ttcaccagaa
    12001 caacagcctc tccgctattc aacaacgtca gcgtccgccc gtttctgttc agttcgatcc
    12061 cgccgctgga tgcgttcacg actgcctccg gcgcgggcct cgcttcgaag ccggctgtgt
    12121 ccaggtttgg ccgcggcatg gttaaccacc aaccagtggc caccagcatg gttgccgaag
    12181 ccagagcggt cacagggcgc catccgagtc tccgttcggg agccggccgc accggcacgc
    12241 actcggcggc cgcaaggccc acccggatgt tggctgtcat ttcgccggcc agccgctgcc
    12301 agttcagtcc ctccggcaat gcttccgcca gattaaccgc cttttcctga gcttcgcgaa
    12361 acgctctcac ctcttcccgg cagcgttcgc accggttcag gtgcaggcgg atccgccagc
    12421 ccgaaaatac accgtggtcg cctcccgcga aaagcgccaa ctcggccagt gcagggtgcg
    12481 tcatggcttt ctcctctcga tataacgccg gaacttgata cgcgcgttcg cgatatgaga
    12541 gcgcaccgtc gccttggaac aattcagttg ccgtgccact tcttcggccg ggagcccctc
    12601 tacatcgcgc agcagcagcg ctgtccgctc acggccgctc agcaggcgca ggccatgatc
    12661 gagataagcc cgttgctcgg agcgaagcag aatgtcctcc gggctttcat acggcggagt
    12721 cgacctcggt tcgggtgcat cgtccagctc gcagaaagag atcctgccgt ggcgccgcag
    12781 aaagtcgatg gccgtgttgc tggcgatgcg cgacagccaa tgcgccgcct tgtccagatc
    12841 tttgagctga tcttcgcgct gcagggcttt aatgaaagct tcctgtgtca ggtcctgtgc
    12901 gtcatcgata ttgccaacca tgcgataaat ttgcaggaaa atccttcgca tgtgctcttg
    12961 aacgaaccgg tcccgccgtt gctgggattc gtccggggag ctttcgaggg gggggcgcgc
    13021 ggcctctctc atcattcggt tcaatccttc tatggcccct gccgggccgc aggctccccg
    13081 gaccggcgcg ggcgggccgg attgggaatg ctccgtcgcg ggagtatgta aaagcctgac
    13141 catgcgccta caaacaggat gacgagcgag ttcggaaaac gtgtagggat tgtcggcgct
    13201 atcctaattg tattatgcgg gatgttgcga ttatcggcat cggcaaaacg ccgtttggag
    13261 cctttcccga tcgtgatctg cggtccctgg ccacggaagc cggttccaaa gccttgacgg
    13321 atgccaacgt caagcctgcc gaggtcgagg cgttctatct ggggaacttc gccggccctt
    13381 cctttattgg ccaaagccat ctcgccccgt atatcgcggc ccctctcggg ctcaccggaa
    13441 ttccggcggt gcgctgcgag gccgcctgcg cttcgagcgg cgctgccttt tttcaggccg
    13501 tctccatggt tggggcggga ttgtacgacc tggtattggt cataggcgtc gagaagatga
    13561 cctcgcaacc tactccccgg gttacagaga ttctggcctc agccggggat ctggaagggg
    13621 aggtgaaggc cggcgccacc ttcccggcgc tgttcgcgat gatcgcccgc cggcacatat
    13681 atcagtatgg gaccacgcgc gagcagatgt cgctggtggc ggtgaagaac cacgccaacg
    13741 gagcgcgcaa tccttacgcg cacatgcgaa aaccggttac gctggaacag gccatggccg
    13801 gccggctggt tgccgaaccg ttgaatctgt acgactgctc cctggtgagc gacggcgctg
    13861 ccgccgtggt ggtggcgccg ctcgaacgcg ccgatgaatt caacggtaag ccggttcgcg
    13921 ttcgcggaat cgcccaggcg tcggatttcg tcgcgctgga ttcgaaagac gacatcacgc
    13981 ggttccgcgc cacccagaag gccgctgaca aggcttaccg catggcggac gccaccgcgc
    14041 gcgatatcca gttcgccgaa gtccatgact gcttcaccat cgcggagatc gtcgcgctgg
    14101 aggaccttgg ttttgttgcc aaaggcgaag gcggccccta tacccaggac ggctgtacag
    14161 cgctcaacgg gccgcggcca gtcaacgcca gcggagggtt gaagagcaaa gggcatccgg
    14221 taggcgcaac cggcgccgga cagctttgcg acctggtcct gcaaatccgc ggcgaggcgg
    14281 gggagaacca ggtggaacgg cactcgctcg gcttggcgca gaatctcggc gggtccggag
    14341 ccactagcgt tgtgaccatt ttgagcgtat gagcgatcgt cctcctctca cggcaggcac
    14401 catctacacg gagacggtag tgcactccgc cccggaagcc ctggtcaacg aggcgccgta
    14461 tcaaatcgcg attgtcgagt tcgattccgg ctcgcgcgtt acgggccgca tcgagggcga
    14521 ccgtgtgtcc atcggcgacc gcgtttcttt ttccgggttc cgcaacgagg taccattttt
    14581 caggaagagc tcatgaaact ggcagaacgt atgtcccgcc tcggcacgga gacggcgttt
    14641 gaggtactgg tgcgcgcccg tgcgctcgaa gcccaaggac gcagcattat ccacctggaa
    14701 attggagagc cggatttcga aactccgcgg catatccgcg aggcggcgaa aaaggcgctg
    14761 gacgaaggct ggacccatta cggccccacg cagggctatc ccgaactgcg cgaagcgatc
    14821 gcatcttcga tctgccacag ccgcgggatt ccggtggacc cctcccatat ctgcgtcgtg
    14881 cccggtggca agcccatcct gttttttccg atgctcgccc tactcgagcc gggtgacgag
    14941 gtgatttatc ccaacccggg atttcccatt tacgagtcga tgatccggtt tctcgatgcg
    15001 gtgcctgttc ccatgccgct ggtggaatcg ctgggcttct ccttcgacct ggatctgttc
    15061 cggcagcgtc tgagcaacaa gaccaaactc gtcatcctca attctcccca gaatccgacc
    15121 ggaggagtga ttccggcgcc ggacctggcg gcgatggcgg agatgttgcg cgatcgcgac
    15181 gtcatggtgc tttccgacga gatctactcc cggatctact acgacgagcc gcccgtgtcg
    15241 atcacccgtt ttccgggcat gctggagaag acgattctgc tcgacggttt ctccaagatc
    15301 tacgccatga cggggtggcg catggggtat ggtgtgatgc ctccgtggct ggtggaagcg
    15361 gtgaacaagc tgatggtcaa ttcgaattcc tgcaccgcca gctttacgca gcgagcgggg
    15421 ctcgcggcgc tgaacggacc gcagggcgaa tccgaaaaga tggtggcgga gttccgccgc
    15481 cgccgcgacg ctttttgcca ggggctcaat tcgctgccgg gcttccggtg cgccattcct
    15541 aaaggcgctt tctacgcatt tccgaacatc gaaggcacgg gaatgcgttc ccgcgaactc
    15601 gccgacgcgc ttctggccga aaccggcgtg gcggccttga gcggcacctc tttcggagag
    15661 tacggcgagg gttatctgcg gttcagctac gcgaattcgc tcgagaattt gatggaagct
    15721 gtggaccgta tccgcaaatt cctgaataat cgctgaatgg aagtctcatt ccagctcaaa
    15781 atgggccagc gcaccatgga cgcgggcgtg aacctgcccg atgtgcccgt ccgtacgctc
    15841 gacctgcttc cggtgctgct gcccttcgcc agcgcaatcg tagatgccag cacagccgac
    15901 gtggaggagg aggggaaaca catttcctgc cgcgcgggct gtggagcgtg ctgccgccaa
    15961 ctggttccca tcagccgccc ggaagctctt tatctggccg cgcatctcga caccctgccg
    16021 ccggaacgcc gcaccatcat ccgcgagcgg ttccaggttg ccgtcgaacg attgcgggag
    16081 acaggcatgc gcgaacggct caccggcttc gaagaccagg ggcaggacgc gcgtagagaa
    16141 atcggcatgg actatttccg cctggggatc gcctgcccgt tcctcgagga ggaatcgtgc
    16201 ggtatttacg agcaccggcc gatggcatgc cgtgaatatc tggtgacgtc gcccgccgcc
    16261 aactgcgcgg acccccatcc cgagaccatc gacacggttc cgctctacgc cagaccttcg
    16321 gttatcctct accgcttcgg cgacggcaag ggagaagacc ggccgcgatt cctgcctctc
    16381 gtgttcgcgc tcgagtgggc ggcggcacat gcggcagata cacaagcgga gttgcccgca
    16441 cccgagatgt tccgcaattt tgtccggaaa gtggcggaat gatcaggcga gaggtggcta
    16501 tttgcggtgc ttctgccacc agacttccgg ccgcccgtcg ggtccgacat gcggattcag
    16561 ctccgtcatc attccgtata actcgggccg ccgcgcccgt atgtaggacg gccccgaagc
    16621 aagctcccgt ttttggggat ccagggttgc caccacgacg tcatcgccca acgtgcggca
    16681 ttcgacgaga acttcgccga acggatccag aatcatggag ccacccggct tgatggtgcc
    16741 gccttccacg ccgatcggat tcgaatagat cgcgaagacg ccgttttccc atgcgcgcgc
    16801 gggaagccag cgcatgatcc agccgcgccc tttcggcccg tcgaattcct gccgcagccg
    16861 taccggatcg cgttcgcggt tgtgccagag ttcggggtcg accgtgcccc ttccgggcat
    16921 cggcgaaggc aggcagccgg tgacatgcgg catcagaata atttccgcgc ccatcattgc
    16981 cgtcagccgt acgttctccg gcaggttgtt gtcgtagcag gtgaggatgc cgaagcggca
    17041 acccagcagg tcgaaaaccg tgtagcgatc tccacacgtc aggtattggc tgataaaagc
    17101 gtggatcttg cggtgcttcg ccacgaaccc gttttcgtct acgacgacgt agcagttgta
    17161 catcctgtcg ccctcgcgtt ccaccaggcc ggcggccagc gcaacgccga gcgagcgtga
    17221 gatctcagcc agcttttcgg tggaaggtcc gccggggacg cattcggcca gcgcaaacag
    17281 ctcttcgcgg gacagcttct cgagaaacgt gtagccggaa atcgagcatt cgttgaagga
    17341 gacgagttgc gctccttctc cggccgcctt gccggacaat tccgcgattc ttttcaggtt
    17401 gtaggctttg tcgccgtcgc gcacttcaaa ctggccggcg cctgcccgta tctctttcat
    17461 cgatattcca gtccgctcaa gccgggtatt ttcccttgat gtagttgctg agctgatcga
    17521 ttgtctccgc ctgggccgag atgatggagt ttaccagatc tccgatcgat accagccctg
    17581 ccagcttgcc ctcttcgagg acaggcagat gacggatgcg gtggcgtgtc atgattcgca
    17641 tgcactcatc gacggtatga tccggcgtca cggtcaccag gtccgtagcc atgatctcgt
    17701 gtactgtggt gtctttcgac cttttgtcct tcagaatcac cttccgcgcg taatcgcgtt
    17761 cggaaatgat gcctaccagc cggccggatt ccgccacgag caaagcgccg atatcgcggt
    17821 cggccagcat aagcagcgca tcatagacgg tcgattccgg cgaaatcgtc cagacctcgc
    17881 cgcccttgcg cctcagaatc gagcatacgg tttccaccat tctgccaatt gctccttacc
    17941 cagattcgtt actcttaata tcagcccgcc aacgccgcgt caagtgtggc accgccatcc
    18001 gcgtacacgc cggatggtat tgtgtgcgtt atgcactcac gtcgcagctt cacaggagcc
    18061 cttctggggt tttccgcctc ccagagtcta ccggccgcgg cggccgatcc ggcgtggatg
    18121 ggaattgcgg aaggcggcgg cctgttacgc cgcaagaagc tctcgcccat agagtggacc
    18181 aaagcctgcc tcgatcgcat cgaaaaactc aacccgcgcc tgaatgcgtt tatcaccgtc
    18241 accgccgaac aggccctggc ggacgcgcgg cgaatggaga gcgagcttca ggcaggcaaa
    18301 gtacgagggc cgcttcatgg cgtgcccatc gctctgaaag acctgatcga cacgaccggt
    18361 atacgcacca
//
LOCUS       DASTMK010000206         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_10938, whole genome shotgun sequence.
ACCESSION   DASTMK010000206 DASTMK010000000
VERSION     DASTMK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10938"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFQ79_09020"
     CDS             <1..656
                     /locus_tag="VFQ79_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0139838.1"
                     /translation="VQRDDNSKKYSVGYKLLAFREGSAARTNLNLRALALPLMEELTE
                     ITGLTCHLATFEKDEAVYIEKAEPAGFIRLNTWVGKRNSLHSTAVGKALLMFTPENQI
                     RKILKDAGLPRKTDRTITTVEAFLEDMARCRERGFAVDDGEDENEGCCIAAPIFSGDD
                     HVVAAVGLSGTVAQIDAQRREAIGKLITNYARQISARSGQLYAVPLPRAAKNINRAG"
     gene            complement(661..1734)
                     /locus_tag="VFQ79_09025"
     CDS             complement(661..1734)
                     /locus_tag="VFQ79_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139839.1"
                     /translation="MNGRPRIGIVGLGDIARLHAQGLLSNSEIQLAVCRGRNPGSADL
                     FARDFGAVVYDNWAQMLDDERVVAVDLCVPNDLHRSYAESAASAGKHVICEKPIAMTS
                     GDGIAMIDACRSAGVQMLVAHVLRFWPEYRIMRDVLQSRQLGACRTVTLRRMLSLLVS
                     VPGREGWRNSPNRMGGAILDLQIHDIDFLLWTFGLPGTIYCAGVRSADGGLNHVYTIL
                     TYPDGPCALIEASYMLKGDPMIFTAKAVCDEGSLDYSMNLAQFEMHEIEEPAQGNGRP
                     GGASLTCFRPSAQAEVLAHSDPQVLENVFRAELAYFADCVLGRTENDVANAEDAIAAV
                     RVVEACRESVRTAGVVRIAAAGV"
     gene            complement(1731..2651)
                     /locus_tag="VFQ79_09030"
     CDS             complement(1731..2651)
                     /locus_tag="VFQ79_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0139840.1"
                     /translation="MTKRYKKGIVIRAFAEEPNFLGGCGFIRSVDDYARIFSRVRGAG
                     FTGVQPYLALEGGVLNLASSTGVLLSIRQFAADEGVDLPSLEIAPLEYSLTSGTETVR
                     ARGVEVVKRAIEVAAELNSAGVLVIPGYVGRMWDSQTDQVDYEDAWNRTVAGLREVVA
                     DAERLKVSVLVEPIWNMFLLSPLEMRRLIDEVASTHCGVLLDTGNVTLFGFAEQWMRI
                     LGHRVQQVHLKDFRRQVGNINGFVPLLAGDVNWPAVVDAARKTGFDGYWIAEQFPFQY
                     HGDSILEATSRAMDRIVGIAAGGALEASSQ"
     gene            complement(2656..3471)
                     /locus_tag="VFQ79_09035"
     CDS             complement(2656..3471)
                     /locus_tag="VFQ79_09035"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU0139841.1"
                     /translation="MQWKIIAAALLAAAADTQTTAVPGRDATRLQALILTGYNMHPWR
                     PMTEVLRQHLEASGRFEVRINEEPAGIGQETLNGYDVVILNYTNYLGRFGPTFPDRSR
                     MALLDHLKRGKGLVAYHASLSSFAEWPEYERMIGGAWRTGSKHAPYHTFRVEIVAPEH
                     PIVAGLPASFMQSDELNQGLHMQKDIAILATAFDDPNNCTEREPKVCGSGKQQPVMWA
                     STYGGGRVFTTALGHDMVSIKSAPFIATFVRGAEWAATGKVTIAPPAELTTSK"
     gene            3620..>4132
                     /locus_tag="VFQ79_09040"
     CDS             3620..>4132
                     /locus_tag="VFQ79_09040"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0139842.1"
                     /translation="MAQNSMRLGTPFLTLTLVLVAALALLSTPVVAQNLTGQISGLIL
                     DPTGKTVPDATVVLTNQETQQQRTVATDQEGRFLFTQLLRGTYTLTITAPGFKQLKKE
                     NVVLTATERLVLRDSILEVGNVTQSVLVTAEAARVATESSERAGVVTSTQIQQLPTKG
                     RDIMDVVRTLP"
BASE COUNT          917 a         1234 c         1139 g          842 t
ORIGIN      
        1 acgttcaacg cgacgacaac tcgaagaaat acagcgtcgg atacaagttg ctggcttttc
       61 gggaaggcag cgccgcgcga accaatctca acttgcgtgc tctggctttg cctcttatgg
      121 aagagttgac ggagattacc gggcttacgt gtcatttagc cacgttcgag aaggacgagg
      181 cagtgtatat cgaaaaggca gagcccgccg gtttcatacg cctcaacacg tgggtgggca
      241 aacgaaatag tctccactcc accgcggttg gcaaagccct gctgatgttc acgcctgaaa
      301 accagattcg caagatcctc aaggatgctg gtcttccgcg caagacggat cggaccatta
      361 ccaccgtcga agcgtttctt gaggacatgg cgcggtgccg ggaacgtggg ttcgcggtgg
      421 atgatggtga agacgagaac gaaggatgct gcatcgccgc acccatcttc tccggtgacg
      481 accacgtagt tgccgccgtc ggactatccg gaaccgtcgc ccagatcgat gcacaacgaa
      541 gggaagccat cggtaaactc attacgaact atgcacggca gatttcggcg cgcagcgggc
      601 agctatatgc cgtcccactg ccgcgtgccg ccaagaatat taaccgggcc ggctaggcat
      661 tcagacgcct gccgccgcga ttcgaacaac gcctgcggtt cgaacggatt cccggcacgc
      721 ctccacaacg cgaacggccg cgatcgcatc ctctgcgttt gcgacgtcat tttccgttct
      781 acccagcacg caatcggcaa agtaagcaag ctccgcccgg aacacgttct ccagtacttg
      841 cgggtccgaa tgagccagaa cctcggcttg cgcactgggg cggaagcagg tcagacttgc
      901 cccacctgga cggccattcc cttgtgccgg ctcttcgatt tcgtgcattt cgaattgcgc
      961 gaggttcatg gaatagtcca gcgagccttc atcgcagacg gccttggcgg tgaaaatcat
     1021 gggatcgcct ttaagcatgt agcttgcctc gattaacgcg caaggaccat ccggatacgt
     1081 caagatcgtg tagacgtgat tcaatcctcc gtccgcactg cgaactcccg cgcaataaat
     1141 agtgcccgga aggccgaatg tccagagcag gaaatcgatg tcatgaatct gcaaatcgag
     1201 aatggcgcca cccatcctgt tcgggctgtt cctccagcct tcccggcctg gcacgcttac
     1261 gagcaacgaa agcatacggc ggagcgtgac ggttctgcat gcacccaatt gtcttgattg
     1321 caggacgtct cgcataatcc gatactcggg ccagaatcgg agcacatgag caaccagcat
     1381 ctggacaccc gcgctacggc aagcatcgat catcgcgatc ccatcgccac ttgtcatggc
     1441 aatcggtttt tcgcaaataa cgtgtttgcc agcggaggcc gccgactcgg cataggagcg
     1501 gtgcagatcg ttcggcacac acagatcgac agcgacgacg cgctcatcat cgagcatttg
     1561 cgcccagttg tcgtacacca cagccccaaa gtcccgcgca aataaatcgg cggaaccggg
     1621 attcctccca cgacagactg cgagttggat ttcggagttg gagagcagtc cttgcgcgtg
     1681 tagtcgcgcg atatctccga gccccacgat cccgattcga gggcgcccgt tcattgcgat
     1741 gacgcctcca aggcacctcc ggcagcaatc ccaacaatgc gatccatagc acgtgaagtg
     1801 gcttcgagta tggaatcccc gtgatattga aaaggaaact gctcggcgat ccagtatcca
     1861 tcgaaccctg tcttgcgggc ggcatccacc accgcaggcc agttcacatc accagccaga
     1921 agcggcacaa atccgttgat gttcccgacc tggcggcgaa aatccttgag gtggacctgt
     1981 tggacacggt ggccgagaat gcgcatccac tgttccgcga agccgaacag agtgacattg
     2041 ccggtatcaa gaagaacgcc gcagtgtgtg ctggctacct cgtcgatgag ccttctcatt
     2101 tcgagcgggc tgagaagaaa catgttccag atcggctcca ccagtacaga gaccttgagc
     2161 cgttcggcgt ccgcgaccac ctcgcgcagg ccggcgaccg tgcgattcca cgcgtcttcg
     2221 tagtccacct ggtcggtctg ggagtcccac atcctgccga catacccggg tatcaccagc
     2281 acacccgcgc tgttcagttc ggcggcgacc tcgatcgcac gtttgacaac ctccacgccg
     2341 cgagcgcgaa cggtttcagt gccggatgtg agcgagtact cgagcggcgc gatctcgagg
     2401 ctggggagat cgaccccttc atcggcggcg aattgcctga tactgaggag cacaccggtc
     2461 gatgaagcaa ggttaagaac gccgccttcc agcgccagat aaggctgcac gccggtgaaa
     2521 ccggcaccgc gcacacggct gaaaatccgg gcatagtcgt caaccgaccg tatgaagccg
     2581 cacccgccga gaaagttggg ctcctccgcg aaggcgcgaa ttacgatgcc ttttttgtac
     2641 cgtttcgtca tcagatcatt tagacgtggt cagttcggcc ggtggagcga ttgtaacctt
     2701 gcccgtcgca gcccactccg caccacgtac gaaagtagca ataaaaggag cgctcttgat
     2761 cgacaccatg tcgtggccaa gcgccgtcgt aaatacacgt cccccaccgt aagtcgaggc
     2821 ccacatgacc ggttgttgct tgccggagcc gcagactttc ggctctcgtt ccgtgcagtt
     2881 gttgggatcg tcaaacgccg tggcaagtat cgcgatgtcc ttctgcatgt gcagtccctg
     2941 gttcagttcg tcggactgca taaacgaagc gggcaatccg gctacaatcg gatgttcggg
     3001 agccacaatt tcaacacgga aggtgtgata cggagcgtgt ttcgatccgg tccgccacgc
     3061 accgccgatc atgcgctcgt actcgggcca ctcagcaaag gaactgagtg aggcgtggta
     3121 ggctacgagt cctttccccc gcttcaagtg atcgagaagc gccatgcggc tgcgatccgg
     3181 aaaggtcggc ccgaacctgc caagatagtt tgtgtagttc aggatgacga catcgtagcc
     3241 gttcagggtt tcctgcccga tgccagcggg ttcttcattg attcgaacct caaagcggcc
     3301 ggaggcttcc aggtgctgcc tgagaacttc ggtcatcggc ctccacgggt gcatgttgta
     3361 gccagtcagt atgagcgcct gaagcctggt tgcgtcacgt cctggaacag cggtagtctg
     3421 ggtgtccgcg gcagccgcaa gtaacgctgc tgctatgatt ttccactgca tcgagcccct
     3481 cccctgaacg gtatccgtca aatcagaata tggatactat atcaaaaaat tgagtacaag
     3541 tgctgttttt taccttttta gacttgactt ctcagcttat tgaggatata tcagaaacat
     3601 tcgctcagga ggagctccca tggcacaaaa ctcaatgcga cttggcacac cctttctcac
     3661 actcactctg gttcttgttg ctgcgctggc tctactttca acgccagtcg tcgcgcaaaa
     3721 cctcactggc cagatttcag gattgattct cgatccaacc ggtaagacgg ttccggacgc
     3781 cacggttgtc ctgacgaatc aggagacgca gcaacaacgc accgttgcaa ccgatcagga
     3841 gggccgtttc ctgttcacac aactcttgcg gggcacgtac acgctgacca tcaccgcgcc
     3901 cggttttaag caactcaaga aggagaacgt cgtactgacc gccaccgagc ggctggtttt
     3961 gcgcgattcc atactcgagg tcgggaacgt gacgcaatca gtgctggtaa cggctgaggc
     4021 agctcgtgtc gccacggaaa gcagcgagcg ggccggagtc gtcacttcca ctcagatcca
     4081 gcaattgccg accaaagggc gcgacatcat ggatgtcgtc cggactctcc ct
//
LOCUS       DASTMK010000207         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_106154, whole genome shotgun sequence.
ACCESSION   DASTMK010000207 DASTMK010000000
VERSION     DASTMK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106154"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VFQ79_09045"
     CDS             complement(<1..1591)
                     /locus_tag="VFQ79_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0139843.1"
                     /translation="MSLPEFSVNRPVTTLMACLILILLGGIAFVRLPVDLMPELVYPT
                     ISVRAEYPGVAPEEMETLVARPLEEAFASAPGVEEITSNSSEGITQVRVGFEYGVDID
                     EAANELRIRLDRRRGTLPEDMEPPVMFKFDVSQRPIMFLTVAATDMNPKTLRYFVEKQ
                     IQYRLERVPGVAQFTVRGGLRREIHVDLDLTKLRALDLSVNHIVDLVRRENLNRPVGP
                     VEEGRFEVLLRTKGEFDDLNEIRNLVLTTRTGIPVYLRDVANVEDSHEETRQLVSVDG
                     APAVRLFVYKQSGANTVTVADAVWDEVDRIHRDYPNVRISATGDTSEFIKASIDNLKS
                     SAIMGSILAVVILLLFLRSFSSVLIIAIAIPISVIATFALMFFAGFTLNTVSFGGLAL
                     GVGMLVDNAIVVLENIFRHRQKGTGAKEAAVRGSGQVGLAIAASTFTTVAVFVPVFFL
                     SGMSVVTFQQLAYVVSFSLLCSLFVALTIVPMLCSKYLRVNEAAARRPGVFGAIYRWM
                     GDVQDRLAERYGNSIQWSLDHP"
     gene            complement(1598..>2237)
                     /locus_tag="VFQ79_09050"
     CDS             complement(1598..>2237)
                     /locus_tag="VFQ79_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0139844.1"
                     /translation="QAQAELNELKIQLQQTRIYAPMAGSVARRYVDPGALLGPSTPII
                     NLVNLSVMVTVANVPERDIGNIRVGNTAIVKVDAFGGEDFQGRVVRISPVLDAATRSA
                     SVEVEIPNRDARLKAEMFARVMLDLATFRESVLIPREALVYRGQQPGVYIPKGNQAEF
                     RPIETGLTQGEQVEVMANLLPGTSIVTSGATMLGEGDEIRVAGGRAGEEKPE"
BASE COUNT          487 a          686 c          617 g          447 t
ORIGIN      
        1 tcggatgatc gagtgaccac tggatggagt ttccgtatct ttccgccagc ctgtcttgca
       61 catctcccat ccaccggtaa atcgcaccaa atactcccgg ccgccgggcc gcggcttcat
      121 tgacacggag atactttgag cagagcattg gcacaatcgt caacgccacg aacaacgaac
      181 aaagcagcga aaacgataca acataagcga gctgctggaa cgtcactacc gacattccac
      241 tcaggaaaaa tacgggaacg aagaccgcaa ccgttgtaaa ggtggacgcg gcaatcgcaa
      301 gtcccacctg tccgctacct ctcaccgccg cttccttcgc tccagttcct ttttgccggt
      361 gccggaagat gttttcgagc accacaatgg cgttgtcgac cagcatgccg actccgagag
      421 ccaggccgcc gaaagatacc gtattgagtg tgaagcccgc gaagaacatc agggcaaatg
      481 tcgcaatcac cgagatcgga attgcaatgg cgatgatgag aacactcgaa aaacttctca
      541 ggaacaacag aagaatcacg acggctagaa tcgaacccat gatcgccgaa ctctttaagt
      601 tgtcgatgga agccttgatg aattccgatg tgtcgccggt ggcggagatg cgcacattgg
      661 ggtagtcgcg atgaatgcgg tctacttcgt cccagaccgc atcggcgact gttacggtat
      721 tcgcgccgga ttgcttataa acgaaaagcc ggaccgcagg ggcgccatcg acgcttacta
      781 attgccgggt ctcctcgtgc gagtcctcta cgttagcgac gtccctcaga taaacgggaa
      841 tcccggtgcg tgtagtaagc accaggttcc ggatctcatt gagatcgtcg aattcccctt
      901 tcgtcctcag cagtacttcg aacctgccct cctctaccgg tcccaccggc cggttcaggt
      961 tttcccggcg caccaggtcc acgatgtgat tgacggagag gtcgagcgcg cggagcttcg
     1021 tcagatcgag atcgacgtgg atctcacgcc gcagtcctcc gcgaacggtg aactgcgcga
     1081 cgccgggcac gcgttcgagc ctgtattgaa tctgtttctc aacgaagtac cgcagcgtct
     1141 tgggattcat gtccgtggcg gccaccgtca ggaacatgat gggccgctgg gaaacgtcga
     1201 acttgaacat taccgggggc tccatgtcct ccggcaacgt accgcggcgg cggtccaggc
     1261 ggatacgaag ctcgttggcc gcctcgtcga tatctacacc gtactcaaac ccgacacgca
     1321 cctgcgtgat tccctcgctg gagtttgagg taatctcctc cacgccggga gccgaagcga
     1381 atgcttcctc gagcgggcgc gccaccaggg tctccatttc ctccggggcc acgccgggat
     1441 attcggcgcg cacactgatc gtggggtaca ccagttccgg catcagatcc acgggaaggc
     1501 ggacaaaagc gatgcctccc agcaggatca gaatgagaca cgccatgagc gtggttacgg
     1561 ggcgattgac ggaaaattca ggaaggctca tggctcgcta ttccggtttc tcctcccctg
     1621 cccgcccgcc tgcgacccga atctcgtcgc cttcgccgag catggtcgca ccgctggtca
     1681 cgatgctggt accgggcagc aggttggcca tcacttccac ctgttcgccc tgcgtcagcc
     1741 cggtttcgat agggcgaaat tcagcctgat tccccttcgg aatgtatact ccgggctgct
     1801 ggccgcgata gaccagcgct tcccgcggaa tcaatacgga ttcacgaaat gtagccagat
     1861 ccagcatcac acgggcaaac atttccgcct tcaggcgggc gtcgcggttt ggaatctcca
     1921 cttcaaccga agcactgcgg gtggcggcgt cgagcacggg actgatccga accacgcggc
     1981 cctgaaaatc ctctcctccg aaggcatcta ctttgacgat cgccgtgttc ccgaccctta
     2041 tattgccgat gtcccgctcc ggtacgttcg ccacggtaac cattacggag aggttcacca
     2101 ggttaatgat gggcgtggaa ggaccaagaa gcgccccggg gtcgacataa cgcctcgcga
     2161 cagaacccgc catgggcgcg taaatccggg tctgctgcaa ctggatcttg agttcattca
     2221 actcggcctg agcctgg
//
LOCUS       DASTMK010000208        34709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_233919, whole genome shotgun sequence.
ACCESSION   DASTMK010000208 DASTMK010000000
VERSION     DASTMK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 34709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34709
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233919"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..435)
                     /gene="mce"
                     /locus_tag="VFQ79_09055"
     CDS             complement(19..435)
                     /gene="mce"
                     /locus_tag="VFQ79_09055"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU0139845.1"
                     /translation="MNGFSGFKIDHLGIAVRSLEIALGFYRDQLGMQASEPETVPAEK
                     VKVSMLAAGGPRIELLEAAASDSVIARFIEKRGEGLHHVALSVPDLDAVVERLRSAGA
                     KILNEPRTGAGGHRYVFVHPSSTGGVLLELIQEYTN"
     gene            complement(413..1318)
                     /gene="meaB"
                     /locus_tag="VFQ79_09060"
     CDS             complement(413..1318)
                     /gene="meaB"
                     /locus_tag="VFQ79_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU0139846.1"
                     /translation="MPEWARQIRSGNVRALARAASAVENRTPEADALLRELFPHTGNA
                     LVMGITGPPGAGKSTLADGIIRLLRREEQRVGVIAVDPTSPYSGGAILGDRIRMQSHY
                     ADPGVFIRSMATRGHLGGLAPATTDMALLFDAAGMDVVIVETVGVGQDEVDIVRLADV
                     TAVVLVPGMGDDVQAIKAGIMEIAGVFLINKSDQPGADRLEREIHAMLGLAHHDGPAP
                     PVVRVVASEEVGIAEALDAIRSVAGSRAGNGRSEHIWRRRLLEMYRERLLNRIPAAEL
                     DGAAHDVALRRKDPYSIVDEWLQRI"
     gene            complement(1318..2460)
                     /locus_tag="VFQ79_09065"
     CDS             complement(1318..2460)
                     /locus_tag="VFQ79_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0139847.1"
                     /translation="MEFDYTPEQLQLRKMVREFAESELAPHVMKWDETQTFPREAIAK
                     LSALGFMGAIFIEEYGGAGLNYIEYSILIEELSRVDPAVGLIVAAHNSLCANHIFTAG
                     SEEQKRRYIPKLASGEWIGCWSLTEPEAGSDAAGTRTTAVRDGDGWVLNGGKTFTTNA
                     HEADVCVAMAVTDRAAAHHGISAFIIDKDTPGFRLGKKENKLGMRASSTGEVRFMECR
                     LPKSQLLGRPGEGFIDSLRILDGGRISIAALSVGLAQGAYDAALGYSKQRKQFGRPIS
                     EFQAIQFKLADMATSIDAARLLVYRAAWMKDHGHRVTRESAMAKLFASEIAVRVADEA
                     LQIHGGYGFIKDYPVEKFYRDVKLCTIGEGTSEIQKLVIARQLLKS"
     gene            complement(2483..3685)
                     /locus_tag="VFQ79_09070"
     CDS             complement(2483..3685)
                     /locus_tag="VFQ79_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139848.1"
                     /translation="MNSRRTFIGQMASGIAGTLAAPASVLGANDRVRLGIIGAGARGM
                     ELVREVISQPNVEFAAFADIYARRLEEARRLAPTARTFLDYRHVLDDKSVDAVLIATP
                     QHLHAEHFIASLDAGKHVYQEKTMAFTVDHAKAMRAAYRKTNGLAVQIGHQSCSSGQI
                     ADATRFADPAAMGKITAIHAHMYRNTPHGKPQWTRPLYPDMTAENIIWNSFLGESPRR
                     EFDPNRYANWRFYWDYSGGNVYENMCHQLAFWYKVLGLQIPAAVTMVGGVFLWKDGRE
                     VPDTMNVAMEHSEEILFSWDSGFGNNQLGSSEDVLGTDGTIARAQQIRYLPQKVNRRD
                     GSELMGQTKTAPAAHMADFLECARTGKEPNCPFDIGYRVSIACRMAVESYRQGRTVRW
                     DPVKEDIV"
     gene            3832..5151
                     /locus_tag="VFQ79_09075"
     CDS             3832..5151
                     /locus_tag="VFQ79_09075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0139849.1"
                     /translation="MKQNVSRRHFMAAVPAAGLVAPMAKAAEKPALLGGKPVRTARFP
                     SWPRFDKTEEEGVLKTLQSGNWFRGNGKNVARFEEAYAAMTGAKHCLATANGTSALLT
                     SLGALGVGAGDEVILPPYTFVATVNVILMLSAMPVFVDSDRESSQIDASKIEAAITNR
                     TRVIMPVHLGGNAADLDAIMAIARKRNVPVVEDACQSHLGEWRNRKVGTYGTTGCFSF
                     QASKNLNSGEGGAILSNDSALIEKCYAFHNNGRGRASGGYDFSYASRGSNLRLAEFQG
                     ALLLAQMKRVEEQAATRDRNALYLSSLLREIPGIKPARMYEGCTRNAYHLYMALYDKN
                     EFAGLPRAKFLKALSAEGIPASAGYSPLNKEPFIKNTLNSRGYQAIYSKKQIAEWEER
                     NRCPENDRLCQEAIWFSQTMLLAGREDMEQIAEAVRKIHTHAKALVA"
     gene            complement(6170..7063)
                     /locus_tag="VFQ79_09080"
     CDS             complement(6170..7063)
                     /locus_tag="VFQ79_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139850.1"
                     /translation="MEIEQESKEQFVRQSETRLDAVVIPAGEAPCTTCAGTAASAPVS
                     HVYAIGRIEARFPRLSAEKEFAQAAGRAATAGQTGPEVLHSVLSKPGNRYLARQMCWV
                     FTVQGLETYILQPRDPADFDRLVEALRPQPSPMDLDAVIGVRGPIAPPEFCNGLMVPI
                     VVFDQVYSFDRVALIQAIPRPEKMTEKQFGAAAAEVLDRILHMTDNAGATDDHRALNY
                     LAMRYPGIYTKAREQFERNFSLTGVEVRPSPLNGARNIADVIFFYTSRTNDFTEKFFV
                     RVDVTEEFPFLVTKLSPYYDR"
     gene            complement(7103..7318)
                     /locus_tag="VFQ79_09085"
     CDS             complement(7103..7318)
                     /locus_tag="VFQ79_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139851.1"
                     /translation="MNKPDEHSDPMVQSEQEPRRLHLPGFTAGEEIGLGDVIKRATSY
                     VGIKPCGACQRRAAALNRRVVITGRRG"
     gene            complement(7324..8199)
                     /locus_tag="VFQ79_09090"
     CDS             complement(7324..8199)
                     /locus_tag="VFQ79_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0139852.1"
                     /translation="MVALEITKLMALMERTSGSPDVKIGLIDGPVMTRHPDLSRERLH
                     EVPGHHGGACTRANSAACLHGTFVAGILSARRGSSAPAICPGCTLLIRSLFIEGAAGG
                     DRMPSATPRELAAAIVDCIDAGAQVINLSLALTRSSSTGERSLEEAVDRATRRGVIVV
                     AAAGNQSTIGSSALTRHPWVIPVAACDLRGRPLSDSNFGGSIGRRGLTAPGAAITSLR
                     AEGQTVTLGGTSVAAPFVTGTIALLWSEFPAAPAARIMLAVTGRSGPRRASVVPPLLD
                     AEAAYQSLLRRNARR"
     gene            8384..10648
                     /locus_tag="VFQ79_09095"
     CDS             8384..10648
                     /locus_tag="VFQ79_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0139853.1"
                     /translation="MAKKRIIAHCMREEELATAVEMMPDGEKTESYVIGDIEESDIPK
                     LQAKGIIVQDIDAGLRPPLRGTEDRFEAFAFAGARAGFGAAEPVVDTTRANYYNLALQ
                     GPLLEEWRKELTDLGVKIIHSAPNHVITVKVEPDKVVDVDSLPFVRAMQLQSAAESLP
                     LPGEVEALSESDMTGAKAMLTFDVLLREPDGMQPVMTWLKQNHVNIAAAQGDKIRLYL
                     LEGSPLIDEIASLPEVEFMEPYVAPRLHNDEARSILGLDGPPADNPGFHFKYEGEGQT
                     VGVADTGIDESHPDFGQRIAGIVALGRPGDHSDPNGHGTHVAGSVLGDGSAANGRLRG
                     AAPKAKLFFQSLLDNRGGLGGLPFRLETLFDEAYQNGVRIHNNSWGAATASVYRVTSN
                     EVDAFVHNHRDMLLVISAGNEGTAADPATGKRNASKGFVDWLSIGSPATSKNALTVGA
                     SRSRRTAGGLSALNYGSVWPDAFAEAPISDEKVSGDINALAAFSSRGPCDDYRVKPDL
                     VAPGTDILSTKSSRAPLRNFWGPYPDNPRYAYMGGTSMAAPLVSGCAAVVREYLLKER
                     NHEPSAALLKAILINSTRALSSPDAMADHAFLPNYHQGFGCIFMPWAIPNELNSGMKL
                     EFFDNWRDTGSRFTRTGQRRRMQFTLEGGGPLRITMAYTDPPGRALQNNLNLFMAEPG
                     ANGRKWMGNEQVPMSLNIPDPTNNVEVIRIDDAPAGNYLIQVTAYNLLQPQPGQDFAL
                     VVTGKLASPLVQIG"
     gene            10653..11759
                     /locus_tag="VFQ79_09100"
     CDS             10653..11759
                     /locus_tag="VFQ79_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139854.1"
                     /translation="MFSIQMVQAGFGDCFLVEFGTPGHPRHILIDGGPPAVYEQHLRP
                     VLKCLAGNGGVLDLVVLSHVDNDHAVGLLDYFAEMRDTPGPNSGLPAISGLWHNSFSR
                     TIDRGSGIERRLQSLMTASRAQVMSQAGNAVNGIREGAALRTAALALGIPINAGFEDG
                     LITVETARRPLKLGNLLITIATPAGLQLEELQTKWREWLDKHEEAIANDEFLVMANSD
                     ASVPNLSSIGFLMEADGKKALFTGDGRSDHLLKGLEQARLLSDGKLHVDLLKLAHHGS
                     SRNTTKKFFRSVTADLYAVSANGRDGNPDLATLIWIVEAAREQGRKITITATNATPSI
                     KQLKEEYNPATYGYKLQILPPARHSIKLSMPHTA"
     gene            complement(11777..12031)
                     /locus_tag="VFQ79_09105"
     CDS             complement(11777..12031)
                     /locus_tag="VFQ79_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139855.1"
                     /translation="MFEIYRETQYTGLYKVVYFTELQDHNKDSEINHALAGEHYFDGF
                     LKNFGKEDAKQLIDGILARLNSGERLNPEQIAAELREHLA"
     gene            complement(12098..14641)
                     /locus_tag="VFQ79_09110"
     CDS             complement(12098..14641)
                     /locus_tag="VFQ79_09110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU0139856.1"
                     /translation="MKRFAFRLFAPVATLIVASGIASGYYHFVHYDTKTAPFLPIYEK
                     FDLASLPGKTIQYYISANGPSKLAPGDSLTSVISQVRAAATAWNIESSDLSLAFGGVS
                     PEGTAWNGPGIDVIFSDEIPPGLIALGGPTKRADLVEDANGRSFVPIVRSLLVFNRDL
                     SQRPSYGDAFYLSAVHEFGHTLGLQHTLTSSVMSTEITRATSKARPLAADDVAAISLL
                     YPKPQFQSATGVITGRVVLAGDGVGLASVVALPPRGQAISTLTNPDGTYRIEGLPPGD
                     YYLYTHPLPPALAAEVSAANIVLPRGPDQEPVPAGPNFETQFFPGTREWQQAGLVSVA
                     AGTITEGLNFSVQRRSAPFIHSVQTYSYPGEYGVKGAFVNFTSQQGFIVAAGAGILSG
                     SGTATRPTEGLTASVIGGGVSVTSLRPFTSEFLVIDLQSGSQLLDGPRHIVFSLDGSI
                     YVLPSAFTVVQKGPPSIDAISLAPGGPQNRTLALSGANLAPDTRYWFDGQPAALVQTA
                     ESNAVVVAPPAPAGHVAVVTALNSDGQTSMFTQLNAPPTYSYDVGLEGISNVTITPDR
                     LPVGAESVIEIGAPGAMFTQGQAQAGFGSADVSVQRVVVLSPGRVAATVLVGASAAAG
                     TSANLTIASGLKWFVQPAAVTLQQADPERLAFTSPIQLMATSGTPSALQPNSNATLRI
                     SNLTQADIAAGLSLTLGDVSVPILAAAPGQIAVRIPNLAAGPALARLTAGDKIALALL
                     EIDTPPPSILSVFGTFAMPDATRPASVGDLLTVIVTGVPDWGTQIASARATVTVGTTS
                     HPVSEVLRLWQEPVTYQMQFRLGSVPSGRQNVTVTIDARTSTPFAIWTR"
     gene            complement(14638..15201)
                     /locus_tag="VFQ79_09115"
     CDS             complement(14638..15201)
                     /locus_tag="VFQ79_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139857.1"
                     /translation="MKIATTMPFRFRLIVALLLAATVSSTATLEKLSVDQMIQKSTIV
                     VRGTVSGSYAAFRGPLIYTHWKIQVQEELKGSAAGSIEVVTPGGRANALEQTFSGSPL
                     LLKGSQYVLFVWRSPSGLHHLVGLSQGALAVKDDSKGAAVVTRMAISEPMLDVRSGKP
                     VRDEGLTLSLDELRQRVRSAAGAEALQ"
     gene            complement(15314..17212)
                     /locus_tag="VFQ79_09120"
     CDS             complement(15314..17212)
                     /locus_tag="VFQ79_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU0139858.1"
                     /translation="MITPNSADSSRSLAEVHGSVPTGQLSIWKRMFAFAGPAYLVSVG
                     YMDPGNWATDLEGGARFGYQLLWVLVMSNLMAILLQTLSARLGIVAHRDLAQACREAY
                     PRPIALVLWFFCEIAIAACDLAEVLGAAIGLNLLFGLPLLAGVIITAADTLFILWFTR
                     FGIRMIEAFILALVTVIGGCFLVQMILAKPSFTEMAAGLVPRLNRDSLYVAIGMLGAT
                     VMPHNLYLHSALVQTRRIGSTTSEKRAACRFNLIDSVVALNAALLVNAAILILAASVF
                     FKRGIVVTEIDQAHLLLAPLLGTAVASVLFAVALLAAGQSSTLTGTMAGQIVMEGFLN
                     IRMRPWLRRLITRMIAVIPAAITIYISGEEGTFRLLILSQVILSMQLPFAVIPLVHFT
                     SDRRRMGEFVSPRWVRALAWTVATIIVSLNLKLVYDTFAEWIGESGSYAVPVGVLLVL
                     LGAGLVALLLWVVFEPFLPAWLRQLGRAPVAIPDTSGALTLPAYRKILVPVDHSDRDR
                     DAISHAAALAQTHGATLYLIHVEEGVTSQVYGELSSTVEVREGAAYLGRLTRSLRERN
                     INAEAVILHSNNPAMEIVKYAKSLQPDLVVMGAHGHKGLKDIVFGTTINSVRHDLSVP
                     FLIVRDEK"
     gene            complement(17240..19519)
                     /locus_tag="VFQ79_09125"
     CDS             complement(17240..19519)
                     /locus_tag="VFQ79_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139859.1"
                     /translation="MQVAEIHHTDRIKPRRWLISITPVLLLFAIVAGFYWKLTLTNEY
                     TWLESPDLANQVLPWYQFQAGEWHAGRFPMWDPYQWYGQPLIGQGQPGAAYPPNWLFF
                     LTSLRNGWLRQSHLNWYFVLIHYLAALFCYWLCRDLGRSRPGSIVAGCAFALGGYIGT
                     TDWPQMLNGAIWAPLVFLYQLRCLRGERVYASAAASGAFLGVAWLSGHHQVPIFLTLA
                     SAGVWLYGLLVRVYERRRTMAAGALFTVVFILASGLQTLPALEYGKAAKRWVGSSEPV
                     GWNDKVPYTVHKTYSVTPDTLTGLILPGRSHNTDVFWGVVALSLAVLGAAALWRILEV
                     RIFTIVAIAGLFTALGGYSLLHGLLYSLVPMVEKARSPSMAILVFHFASAVLVAYGFD
                     TLLNPDSLWSRRVAIALAVFGLSVLALATALSATYKLAGDTRWILSGLVAVLMSAIIS
                     ARRTGKLGESALAVAIIAVALADIGNGTSYSFVSTEEKDRTIYLKKIAQSADVAGFLQ
                     KQPRPLRVEVADADIPYNFGDWYGIEQSGGYLASITTNLVAHEFYFARFRDLLSVNYS
                     VVNDPPRPGALREGAVEVYYSPASKLKVYFNPSAYPLVRSVHNIVTAGDGAITDLVRQ
                     DGLDLRTTAILREPPPEIARCDAGEDRFRITRRVPGSVDITADMKCAGLLVLADTYFP
                     GWKAWVDEEPAHIYDAYGAIRAVAVPEGTHTVTFRYRPASVFAGAAMSASGLLAAMLL
                     TLVRKPVRHIDAKPTVQSY"
     gene            19618..21939
                     /locus_tag="VFQ79_09130"
     CDS             19618..21939
                     /locus_tag="VFQ79_09130"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEU0139860.1"
                     /translation="MRATYRMLLLMMAGLASYTWADEDEGPGRGVARISVINGEVSVR
                     RGDSGDLVAATINAPLVVQDTVLSGRSSRAEVQFDYANMIRLASMTEIRLAELEYERY
                     LVQVVQGTVTFRVLRDTGAETEISTPSVSVRPIKKGAYRVTVQPDGSSEITVRSGEAE
                     IYTPRGTERLHSGRTMMARGSPSDPEFTVANDIPHDDWDRWNESRDRDLERARSYQYV
                     SRDVYGAEDLDGHGRWVSVAPYGWVWSPRVAVGWAPYRHGRWVWIDWYGWTWVSHDPW
                     GWAPYHYGRWFHSGSYGWCWYPGQIRARHYWRPALVAFFGWGRHSGVHIGVGFGSVGW
                     VPLAPYEPYYPWYGRGYYRGYRDRVYVDNSVRVVNNINITNVYRNARVRNGYTAVDAY
                     DFTQGRAGRYVDTAGVDLRRANLVKGQIPVAPSRDSLRLSDREVRAGNLPPSRAGDRF
                     FSRRTPQTIERVSFEDQRRSMEEVARRTVPDRGGAPGGTRSEAAAGARPGATGFVQGR
                     AGQVETGRTGNTTGWRSVSGSGGTERSDTQARPGNANEGWRRFGEPAGPGSTRAERSA
                     AQPGRTGQDGYRQIGEAGGRTEADGWRRFGNPATGNRSSNPGVRQAEPDASRGRVERT
                     EGASAPARSDTSNRRRFESGAGSAARGGGGGTETSGRVSRSDSTGGARSERSEPSRVT
                     PRNEGSRGGSRLSASEPIRINPPIVRERAPSRSDGGSRVGRSDMPRGGSSGGARVERS
                     TGGGGGGRSSGGASRSSSGGGGGGRESRGGRGR"
     gene            22581..22976
                     /locus_tag="VFQ79_09135"
     CDS             22581..22976
                     /locus_tag="VFQ79_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139861.1"
                     /translation="MLRATTKNTPDPDLIPMARQWLHGYMVKLGREKDRHPPDDRLVA
                     QFLSIAPWPQLERLLYDLLAEHREPGYQYAWFVTVALQRIHGIQPKQLQQRRAMLKLI
                     KPAAEQAAKEGQEWAQLLIGQVASNKTLK"
     gene            23004..23312
                     /locus_tag="VFQ79_09140"
     CDS             23004..23312
                     /locus_tag="VFQ79_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139862.1"
                     /translation="MDPFALIQQLHHQLDLLTRAIHQPPGTAISVGDLVQLAPESHPV
                     YGGYLMDVRNVSETRAGGPILATYRGAWTSIPLDRLVRVSALRAAAIARRKAHEARKA
                     "
     gene            23309..23656
                     /locus_tag="VFQ79_09145"
     CDS             23309..23656
                     /locus_tag="VFQ79_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139863.1"
                     /translation="MTTTPQLKGGRVGMAPEIELLTIGDLHVRLVHYMRALVRHGFLS
                     EAGLARRTHLSQPHIHHVLAGRRALTAASADLIIESLGLGILDLVTTAELAAYLDELT
                     RRVPAADVPPAVQ"
     gene            complement(23610..23795)
                     /locus_tag="VFQ79_09150"
     CDS             complement(23610..23795)
                     /locus_tag="VFQ79_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139864.1"
                     /translation="MTTIDEQLQSLMERRMAVERLIEALERYQRATGARLLPLCPVAE
                     RCATARRAGRPRLERVA"
     gene            complement(23944..24288)
                     /locus_tag="VFQ79_09155"
     CDS             complement(23944..24288)
                     /locus_tag="VFQ79_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139865.1"
                     /translation="MRLVGKVKQMEAARAKRASAVEAQGIVWTEQEELLERPLAAGEH
                     LAVDVEVISDATGEVPAVWRTRERATRTPGDYGVVTCCGVRVGRVMAIAGGLLGIDYD
                     GDAAEQAARGRR"
     gene            complement(24296..24802)
                     /locus_tag="VFQ79_09160"
     CDS             complement(24296..24802)
                     /locus_tag="VFQ79_09160"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HEU0139866.1"
                     /translation="MADKTKARQEGTAKRTAAGRFTPGASGNPAGRPRGARNRASLLC
                     QELLDGSAEAITARLVGLAKRGDRAALRLVIERVLPVRRDRVVEALELPKIRKAEDLV
                     QAASVVVAAAAAGQLSLEEARGFMALIEAERRAIETADLAVRIEALERAGVDDGGLRD
                     ELDRLLKG"
     gene            complement(25473..25691)
                     /locus_tag="VFQ79_09165"
     CDS             complement(25473..25691)
                     /locus_tag="VFQ79_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139867.1"
                     /translation="MSEASRGAARRGGLGVIKSEEREAQPNPVVKRAAHAAAKPHWRP
                     RERHGVWEGREARSGRERRGPGESARVL"
     gene            complement(25998..26219)
                     /locus_tag="VFQ79_09170"
     CDS             complement(25998..26219)
                     /locus_tag="VFQ79_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139868.1"
                     /translation="MVRLRNLGRLPGPHRMEFMRMGREAVAAGGMTGGGPRLAVALSP
                     QSERHARPRSHNCCCASVTECWSGGRERL"
     gene            26481..27023
                     /locus_tag="VFQ79_09175"
     CDS             26481..27023
                     /locus_tag="VFQ79_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139869.1"
                     /translation="MNTQREKVQFTPGETVILALEFDPPADPRPGRFGDQYMYWFAGD
                     KVAWLDPPVHAQIVATGARAGDEIAITRAEVRNGARKRVEWQVQRVEDEPADAYTRQL
                     PPEHRPTPPTAPAQPAAEPRQVAPPRPTAELASALIAAIDAAGVAERYAREQGLAIRF
                     TGEDIRALANAIFTRPERSL"
     gene            27112..27261
                     /locus_tag="VFQ79_09180"
     CDS             27112..27261
                     /locus_tag="VFQ79_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139870.1"
                     /translation="MTNEQLASINQRFDDMRDLWRAELRRLEEVLDARIKQLDARLKH
                     LEGRI"
     gene            27258..27467
                     /locus_tag="VFQ79_09185"
     CDS             27258..27467
                     /locus_tag="VFQ79_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139871.1"
                     /translation="MTIDERLEALTQTVEIIAGMQQETERLMQESDRRTEARIAQLTE
                     TMNRLANIVIRHEERLDQLDGGDPQ"
     gene            27464..27781
                     /locus_tag="VFQ79_09190"
     CDS             27464..27781
                     /locus_tag="VFQ79_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139872.1"
                     /translation="MTDQFIIAIAVAPVLSLVIVVAGYIVQNSNLNARMAEFAIRVGD
                     TRSELRSALEQDRSALRELLRAELSAVRAEMARNQSELLSKFAELDHRLSTLESRFRI
                     ERP"
     gene            27797..28033
                     /locus_tag="VFQ79_09195"
     CDS             27797..28033
                     /locus_tag="VFQ79_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139873.1"
                     /translation="MTSRWRNYAAPIIRRVLADTAGRPEAEVRAALRAAYPFGPRRNH
                     PYKIWLDEIAAQTGRQRRKRRHPSDPQQTFLSFL"
     gene            complement(28244..29572)
                     /locus_tag="VFQ79_09200"
     CDS             complement(28244..29572)
                     /locus_tag="VFQ79_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139874.1"
                     /translation="MRALKALILLSAAAVCAAAETRLEVIAPSNVFAGSKLRAKVIVR
                     MDAAPVSFSHLYVEQMPAGVAMSYAHLAPLHRCAKDGRSFVYNVQPGSAFLVEVEAGA
                     EPGRREAVIAMRLQPSAGGGVATAPGEAVELRIPYRAVSLRGALPPLDITRWESLAVS
                     LGRKWCDGRTYGFGNESEVWYYDGARVYFQLADWTGDKSWEACAFAIAAQYRDNLLSR
                     NGGIQGWRVFPHGLVMAYERSGDDSYRQAALLMARNSTFAARAGDPNDEYIRETAYMV
                     QAWIAAERLGEPRRPELARAVDYLLGHFDRLFVTGSFRLHQTFFDGLAAEALIAWYEH
                     SGDPRVVPAVRSMLDWLWEHGWDSEAGRMVYNPEPPGARCPNSCQSYGRRWLNLILPA
                     YGWYWAVTGDREYIRRGDELWGWALEDEISWSGKEYSQNYRWSFDYLRWR"
     gene            complement(29592..30983)
                     /locus_tag="VFQ79_09205"
     CDS             complement(29592..30983)
                     /locus_tag="VFQ79_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139875.1"
                     /translation="MKRFLLIVMLALPLCAQKTVPWFSGTGGAKVGPGSPPLTCQVGE
                     LFLQTSSTSIYGCTERDTWTLFSGEGWALPEPTVSAAAAVILDYETGQVVWSKAPDVE
                     LKQASTTKLMTALLAHEYDPELDDQVTVSATAAATVGTRAELVTGEVIAMSELLRGLM
                     LPSGNDAAVAIAEHVGRTYLGGTDGATGYAAFIARMNSRAGELGLGHTAYATPHGMDA
                     AGHYTTARELAALGRVYLGVPALRSISATLSYAGTAVSGGPANPHNWKNTNRALGQYP
                     GTISGKTGTTVQAGQCLMALHQMGGNAYIVAILGSTDRYQDATAMWEYAHTLAGTEPE
                     GLTGRSNTAHVDLTGSWPTTSYFPGSYPTNGYGIYSSSAGATATYRFWGTGVQVVTSR
                     SSARGRFDVALDGGTAEMVELYSATATPGVVVWEVQDLPLGMHTIVLTVRADRHASSS
                     ANTCPLEDFVVTR"
     gene            complement(30980..31279)
                     /locus_tag="VFQ79_09210"
     CDS             complement(30980..31279)
                     /locus_tag="VFQ79_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139876.1"
                     /translation="MAIHRLVRPQFAAGAEALVCVDGTRGVNKIRIQAPVTNSGRIYL
                     GDAADMTPETAWGELPPGYTEWLGDESYGNVLDVGAVYVLRETDGDQIRGAAFTR"
     gene            complement(31573..32496)
                     /locus_tag="VFQ79_09215"
     CDS             complement(31573..32496)
                     /locus_tag="VFQ79_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU0139877.1"
                     /translation="MARLRREREELAAAKRAPNSVRAYGFAWADFEGWCREAGRRPLP
                     ASGEAVELYLVDRSKVYKPATLELRLAAIRARHEAAGHKSPVSEETRELLGAIKRRRK
                     NSGPAGKAALTPEELFKASKALRWTARDVRDRAVLVLGFATGLRRSELAALELSDVRF
                     VEQGLVVHVGRSKTDQEGDGVSLGIFPGRRARTCPVRMLRRWLKVRGTAPGPLFHPFT
                     PRGERLDRSRGLGAGSILDIVKRCVALIGLDPAEYGAHSLRAGMVTAAIVDGVPEAVV
                     MRRSRHRSRAVMGRYVRIADLFRVNPLARAL"
     gene            32983..33243
                     /locus_tag="VFQ79_09220"
     CDS             32983..33243
                     /locus_tag="VFQ79_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139878.1"
                     /translation="MTNEQLYLAMAVPILFNGLMFALLLAYMNAKFKSIEERFNERFD
                     AINQRFESINQRFDDMRDLWRAELHRVEEVLDARLKHLEGRI"
     gene            33240..33437
                     /locus_tag="VFQ79_09225"
     CDS             33240..33437
                     /locus_tag="VFQ79_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139879.1"
                     /translation="MTIDERLEALTQTVEIIAGMQQETERRMQETERRMAQLADTMNR
                     LANVVIRHEERLDELDGGNRQ"
     gene            33738..>34709
                     /locus_tag="VFQ79_09230"
     CDS             33738..>34709
                     /locus_tag="VFQ79_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0139880.1"
                     /translation="MKTAFFFLFITVVLWGQERLVTPDDPYFKYQISFDNPGGTLTIP
                     QLSFRLSPTETYTTQRGLDHNMQRAWALTTGSKNIVVAILDDGFFYQHEDVKDNIWRN
                     PGETGVDANGLRKETNGVDDDGNGYVDDVMGWDFIFRDPDPDPYVFDGMDTTRIQPNW
                     HSIAAMGIIGAKGNNAIGVAGINWDISMMLLKRGAQGRKMGDPDPEEVGRVVRAIRYA
                     VDNGARVINWSGYTEDQRPASVAALKAAFEYADRHGVLIVIGAGNEMKNLEDPANCIY
                     LPQCFEMDNLLSVAQLAFEGDLFRFKGSEGSYVSGSNFGPRYVHIAAI"
BASE COUNT         7010 a        11319 c        10223 g         6157 t
ORIGIN      
        1 gaattcctat ctgtgctttc aatttgtata ctcctggatc agttctaaca gcactccgcc
       61 tgtcgacgac ggatggacaa acacataccg gtgtccgccc gcgcccgtgc gcggctcgtt
      121 caggatcttc gctccggctg aacggaggcg ctcgacaacc gcgtcgagat cggggacact
      181 gagcgccacg tgatgcaacc cctcgcctcg cttttcgatg aaccgggcaa taacggaatc
      241 cgaagccgcc gcttcgagca attcgattct cggtccgccg gcggcgagca ttgacacctt
      301 cactttttcc gccggcacgg tctccggttc gctcgcttgc attccaagct ggtcgcgata
      361 aaagccaagc gcgatttcga gcgaacggac agcgatacca aggtggtcga tcttaaatcc
      421 gctgaagcca ttcatcgacg atcgaataag gatcctttct gcgcaatgcc acgtcgtgcg
      481 cggcgccgtc caactccgcg gccggaatcc ggttcaacag ccgctcccgg tacatctcga
      541 gcaggcggcg gcgccagatg tgctcgcttc gcccgttccc ggcgcggctt ccggcgaccg
      601 agcgtattgc atcgagagct tcggcgatgc caacttcttc ggacgcgacc acacgcacaa
      661 ccggaggcgc ggggccatcg tgatgagcca gacccagcat cgcgtgtatt tcacgctcca
      721 agcggtccgc gccgggttgg tccgatttgt tgatcagaaa gacgccggcg atctccatga
      781 tacctgcctt gatcgcctga acgtcgtcgc ccatcccggg caccagcacg acggcggtga
      841 catcggcgag ccgtacgatg tctacttcgt cctgccccac gccgaccgtc tctacgatta
      901 cgacatccat gccggcggca tcgaacagca gcgccatgtc ggtagtagcc ggggcaaggc
      961 cgcccagatg gccgcgcgtt gccatggaac ggatgaacac gcccggatcc gcatagtggg
     1021 actgcatacg gatgcggtcg cccagaatgg cgccgccgga ataagggctg gtggggtcca
     1081 cggcaatgac gcccacacgc tgctcctcgc gccggagcag acggatgatg ccatcggcca
     1141 gcgtgctttt gccggcgccc ggagggccgg tgatacccat caccagcgcg ttgcccgtat
     1201 ggggaaaaag ctcgcgcagg agagcgtctg cctcgggcgt cctgttttcg acggcgctcg
     1261 cggcgcgtgc cagggcccgc acattgccgc tacggatctg tcgcgcccat tccggcatca
     1321 gctcttcagc agttgtcttg cgatcaccag cttctggatt tcactggtgc cttcgccgat
     1381 ggtacacagc ttcacatcgc gatagaattt ttcgacgggg tagtccttaa tgaaaccgta
     1441 gccgccgtga atctgtagcg cttcatccgc cacccggacg gcgatctcgg aggcgaaaag
     1501 cttcgccatg gcggattccc tggtgacgcg atgcccgtga tctttcatcc acgccgcgcg
     1561 gtataccagc aggcgggcgg cgtcgatgga agtcgccata tcggccagtt tgaactgaat
     1621 cgcctgaaat tccgagatgg gccgcccgaa ctgcttcctc tgcttcgaat agccgagggc
     1681 cgcgtcgtaa gcgccctgcg ccaatccgac cgataatgcc gcgatggaaa tgcgtccacc
     1741 gtccagaatg cggaggctat cgatgaaacc ttcgcccggc cggccgagaa gctgcgactt
     1801 aggtagccgg cactccataa atcgcacctc gccggtggag ctggcgcgca tacccagctt
     1861 gttttccttc ttgccgagcc ggaacccggg tgtgtctttg tcgatgatga acgcggagat
     1921 gccatgatgt gccgccgccc ggtctgtcac cgccatggcg acgcagacgt cggcttcgtg
     1981 cgcattagtg gtgaatgttt ttccaccatt gaggacccag ccgtcgccgt cgcgcacggc
     2041 cgtcgtgcga gtgcccgcgg catccgaacc cgcttccggt tcggtaagag accagcagcc
     2101 gatccactcc ccgctggcga gcttcgggat atacctgcgc ttctgctctt cgcttccggc
     2161 cgtgaagatg tggttggcac acagcgaatt gtgcgccgcc acgatgaggc cgacggccgg
     2221 atcaacgcgc gagagctcct cgatcaggat ggaatactcg atatagttca gtccggcgcc
     2281 accgtactct tcgataaaaa tcgcgcccat gaaacccaat gccgagagtt tcgcgatcgc
     2341 ctcgcgcggg aaggtctgcg tttcgtccca cttcatgacg tgcggcgcaa gctccgattc
     2401 ggcgaattcg cggaccatct tcctgagctg taactgctcc ggggtatagt cgaattccat
     2461 agcgtcagtg agtggtgtgc agctagacga tgtcttcctt cacggggtcc cagcggacgg
     2521 tgcggccctg ccggtaactt tccaccgcca tgcggcaggc aatcgacacg cgatagccga
     2581 tatcgaaggg gcagttcggc tctttaccgg tgcgggcgca ttccagaaaa tcggccatgt
     2641 gcgcggcggg ggccgttttg gtttgtccca tcagctccga gccatcccgg cggtttactt
     2701 tctgcggcag gtaacgaatc tgctgcgccc gggcaatggt gccgtccgtt ccgagcacgt
     2761 cctcggagct gccgagctgg ttgttgccga agcccgaatc ccagctaaac aaaatctcct
     2821 cggaatgttc catcgcgacg ttcatcgtgt cgggcacctc acggccgtcc ttccaaagga
     2881 atactccgcc gaccattgtc accgcggcgg gaatctgaag gcccagcact ttgtaccaga
     2941 acgcgagctg gtggcacatg ttctcataga cgttgccgcc cgaataatcc caatagaacc
     3001 gccagttggc gtaccggttc ggatcgaact cgcgccgggg cgattcgccg aggaacgaat
     3061 tccagatgat gttctcggcc gtcatgtccg gatagagcgg gcgggtccac tgcggcttgc
     3121 cgtgcggcgt attccggtac atgtgcgcgt ggatggcggt aatctttccc attgccgcgg
     3181 ggtcggcgaa gcgcgtggcg tcggcgatct gtccggaaga gcaggactgg tggccaatct
     3241 gtacagccag gccgttcgtc ttccggtagg ctgcgcgcat cgccttcgcg tgatcgacgg
     3301 tgaacgccat cgtcttttcc tggtaaacgt gtttgccggc gtccagtgag gctatgaaat
     3361 gctccgcatg cagatgctgc ggggtggcga tcaggaccgc atcgaccgac ttgtcgtcga
     3421 gcacgtgacg gtaatcgagg aaggtcctgg cagtgggggc cagtcttctg gcttcttcca
     3481 ggcggcgggc atagatatcg gcaaaggctg caaactcgac attcggctgc gagatgacct
     3541 cgcgcacgag ttccatacca cgagcgccag cgccgatgat gccgagccgg acgcggtcgt
     3601 tagctcccag aaccgaggcg ggcgcggcca gcgtaccggc aattccggag gccatctgcc
     3661 cgatgaatgt gcggcgtgaa ttcatacttc tattcctttg gattggaaag ggtagtttca
     3721 tcttagcatc gccctcggca atgccactcc ccgttcaatt gccagaagtg ctaatgtggc
     3781 aaactggagc gagagatcct caatccaagt ggcttactac aggaggccga attgaaacaa
     3841 aatgtttccc gcagacattt catggccgcc gtgccggcag cagggttggt agcacctatg
     3901 gctaaggcgg cggagaagcc cgctttactt gggggcaagc cggttcgcac ggcacgcttt
     3961 ccgtcctggc cgcgcttcga caagacggag gaagagggcg ttttgaagac gctccaaagc
     4021 ggcaactggt tccgcgggaa cggcaaaaac gtcgcccgct tcgaagaagc ctatgccgcc
     4081 atgaccggcg ccaagcactg cctggcaacg gcaaacggga ccagcgcgct gctaacgtcg
     4141 ctgggtgcgc ttggagtagg cgcgggcgac gaagtgattc tgccgcccta cacgttcgtc
     4201 gcgaccgtga atgtgatcct gatgctgagc gcgatgccgg tgttcgtcga ctccgaccgg
     4261 gaatcgtcgc agatcgacgc aagcaagatc gaagcggcca tcacaaaccg cacgcgcgtc
     4321 atcatgccgg tccatttggg cggcaacgcg gccgacctgg atgcgattat ggccatcgcg
     4381 cgcaagcgca atgttccggt ggtggaggat gcctgccagt cgcacctggg cgaatggcgc
     4441 aaccgcaaag tgggcacgta tggcacaacc ggatgcttta gctttcaagc gtcgaagaac
     4501 ctgaactccg gggagggcgg cgcgatcctc tccaacgact cggccctcat tgagaagtgc
     4561 tatgcgtttc acaacaacgg acggggcagg gcatcgggcg gatacgattt ctcttatgcc
     4621 agccgcgggt ccaatctccg gcttgccgaa ttccagggtg cgctgctgct ggcgcagatg
     4681 aagcgcgtgg aagaacaagc ggccacgcgc gaccgcaatg cgctgtacct gagcagtctt
     4741 ttgcgtgaga ttcccggcat taaacccgcc cgcatgtacg agggctgcac tcgcaacgcc
     4801 tatcaccttt acatggcgct gtacgacaag aacgagtttg cgggattacc gcgcgccaaa
     4861 ttcctgaaag ccctttctgc cgagggcatt cccgcatccg ccggctactc gcccctgaac
     4921 aaagagccgt ttatcaagaa cactctcaac tcgcgcggct accaggccat ctactccaag
     4981 aagcagatcg ccgagtggga agagaggaac cgctgtccgg aaaacgaccg gctatgccag
     5041 gaagccatct ggttttcgca gaccatgttg ctcgccggcc gcgaggacat ggagcagatt
     5101 gccgaagcgg tacggaagat tcacacccac gccaaagcgc tggtggcgtg agtgccctgc
     5161 gggagccggc ggcgcttcgc cggctaagga tgcaggaaaa tgagggtaag ttccaaagcg
     5221 gtcagtatct gccttctaat atgctggatc gctggtgcgc aggtaacaac taagaaggaa
     5281 tggtcaggcc ttaccgcatt atcaagcgga gagactatca aagtgaagtt gcaacatggt
     5341 aagccagtga ctggcgggtt cgccggcttg catgatacgg acatcgtggt gaggactcga
     5401 cagggtgaac gtgtcatccc caggactgat atccagagaa taagtgtgcg gcgccgggcc
     5461 gcgaagtacg cgccactgat cggattggcg acggggttcg gcgtaatggt tggcatctta
     5521 ggcaatggag gggatttggt tgcttcggga tatgcagctt atctcggtgc cggtgctggg
     5581 attggctacc tgggcggatg gctcgtaggg aaagccgcct ctgacaaggt cgtgtacgat
     5641 gcctccccat gacgatgctc cccggtgcgg ctgccgggcc atctggtggc gtgaaaaggt
     5701 caaacttgct ggcgcgcgcg gctcggtaga atcgctgaga gttaccgagc cgcgaccgtg
     5761 agcctagcac gcgaatgtgg tcgtatcgca atcggctctg acacaggttt ttgatcttcc
     5821 tgtttcagat tccaaatcgc ggcggcacgg agtgcaggcc ggacggcagc gcggaccacc
     5881 accgccgcca accccaccac ccccgccgga cccgtgctca tcactggcgc cgatatggtc
     5941 catccggtaa caggtgtccg cgcagaaagg gtcgctgccg gcaagggaac agccttggag
     6001 gcaaagaagc agatcgagtt ggcgcttgag ttgtggcact acggcctgtg tgcccgccgg
     6061 cgcgccggag tggccgacgg ctgcacgata gcggccgctg gagctgtaaa gcgatatgct
     6121 cgccgtaaat ccgggaatgc tcattgtgtt ctcctgttgg tttctcagtc taacgatcgt
     6181 agtaaggcga caacttcgtc accaggaagg ggaactcttc ggtgacatcc acccgcacaa
     6241 agaatttctc cgtgaaatcg ttggtgcggc tggtgtagaa aaagatgacg tcggcgatgt
     6301 tacgcgcgcc gtttaagggc gacgggcgca cttcgacacc ggtgagcgaa aaattccgct
     6361 cgaactgctc tcgcgccttg gtgtagatcc cgggatagcg catggccagg tagttcaacg
     6421 cgcggtgatc atccgtggcc ccggcgttat ccgtcatatg aaggattctg tcgagcactt
     6481 ccgccgccgc cgctccaaac tgtttttcgg tcattttctc gggccgcgga atcgcctgaa
     6541 tgagcgctac acgatcgaag gagtacacct ggtcgaatac gacgatcgga accatcagcc
     6601 cattacagaa ctccggcggc gcaatgggcc cccgcacgcc aatcacggca tcgagatcca
     6661 tcggacttgg ttgtggacgg agggcctcca ccagccggtc gaaatccgcg ggatcgcggg
     6721 gctgtaagat gtatgtctcc agaccttgaa ccgtaaacac ccagcacatc tgccgggcaa
     6781 gatagcgatt cccaggtttg gagagaacgc tatgaaggac ctccgggccg gtctgtccgg
     6841 cggtggcagc ccgtccagcc gcttgcgcga attccttctc ggcggaaagg cggggaaagc
     6901 gcgcctcgat acgtccgatg gcgtacacgt gcgagacagg cgcagaagcg gctgtgcccg
     6961 cgcatgttgt gcacggcgcc tcgccggcag gtattaccac ggcatcaagc cgggtctcac
     7021 tctggcgcac gaattgttct ttcgattcct gttctatttc cataggtaaa cctcctcgca
     7081 aaatcagtct gtaacgccgg agttacccgc gccggcccgt gatgaccaca cggcggttca
     7141 gcgcggcggc ccgccgctga cacgctccgc agggcttgat gcccacataa gatgtagctc
     7201 gcttgatgac gtcgccgagc ccaatctcct cacccgcggt gaatccgggc aggtgcaggc
     7261 ggcgtggctc ttgttcgctc tggaccatgg ggtcgctgtg ttcgtctggt ttgttcatgg
     7321 atttcatctc ctcgcgtttc ttctcaaaag ggattgataa gccgcctccg catcgagcag
     7381 gggcggaact accgaagctc gccgcggtcc ggacctaccg gtgacggcca gcatgatccg
     7441 ggcggccggc gcggcgggaa attccgacca cagaagtgca atcgtcccgg tcacaaacgg
     7501 cgccgcgacg ctggtgcccc cgagcgtgac cgtttgtcct tccgctcgca aactggtgat
     7561 ggcagctccg ggcgcagtta aaccacgcct gccaatcgag ccgccgaagt tcgaatcgct
     7621 caacggtctg ccccgaaggt cgcaagctgc cacgggaatg acccagggat gcctggtaag
     7681 agcggaactg ccgatggtgc tctggtttcc cgccgccgcg acaacaatca cgccccgccg
     7741 tgtggcccgg tctaccgctt cttccaaaga ccgttctccc gtagaggaag accgcgtgag
     7801 ggccaggctc aggttgatca cttgcgcgcc tgcatcgatg caatcgacga tggctgccgc
     7861 aagctcccgg ggtgtagcgc tcggcatgcg atcaccgccc gccgctcctt cgataaagag
     7921 ggaacgaatc agaagcgtac agccggggca gatggcgggt gctgaagagc ctcgccttgc
     7981 ggacaggatt ccagccacga acgtcccgtg caggcaggcc gcgctgttag cccgcgtgca
     8041 tgctccgcca tgatgacccg ggacctcgtg caggcgttcc ctcgacagat cgggatgccg
     8101 ggtcataaca ggcccatcga tcagcccgat tttcacgtca gggctgccgc tggtgcgttc
     8161 catcaacgcc atgagtttgg tgatctccag ggcgaccata gagtccttga gtaccgcctt
     8221 cgttatcgaa tccgctgtta tttctattgg cgagaaaaag ggggaaaatt agcgagcttt
     8281 cggaaaccaa gccagcctgg cagtaccagc cggcagtacc cctaacctat ccacatggaa
     8341 accatttcga tatgctaaag ccatcgtgat ggagaaaagt tgaatggcga agaagcgaat
     8401 tatcgcgcat tgcatgcgtg aggaagaact cgccaccgcg gttgagatga tgccggatgg
     8461 agagaagacg gagagctacg tcatcggcga tatcgaggag tcggacatcc cgaagctgca
     8521 agcgaaaggg atcattgtcc aggacatcga cgccggtctg cgcccgcccc tgcgcggcac
     8581 cgaagaccgg ttcgaagctt tcgcgttcgc gggtgcgcgc gccggcttcg gcgccgcgga
     8641 accggtagtc gatacgaccc gggcgaatta ctacaacctg gccttacagg gcccgttgct
     8701 ggaggaatgg cgaaaagagt tgacggacct cggcgtcaaa atcattcaca gcgcacccaa
     8761 ccacgtgatc acggtgaaag tggaacccga caaagtcgtg gacgtagaca gtctaccttt
     8821 tgttcgtgcg atgcaattgc aatcggccgc ggagtcgctg ccgctccccg gcgaggtgga
     8881 agccctgtcg gagtcggaca tgacgggcgc caaggcgatg ctgacgttcg acgtcctgct
     8941 gcgggaaccg gacggtatgc agccggtgat gacgtggctg aagcagaatc acgtgaacat
     9001 cgcggcggcg caaggcgaca agatccgttt gtacctgctg gagggctcac ccctgatcga
     9061 cgagatcgcg agcctgccgg aagtggagtt catggagccg tatgtcgcgc cgcggctgca
     9121 caatgacgag gcacgctcga tcctgggact cgacggtccg ccggcggata accccggatt
     9181 tcatttcaag tatgagggag aaggacagac ggttggtgtg gccgatacgg gcatcgacga
     9241 gtcgcatccg gacttcggcc aacgcatcgc cggcattgtt gcgctggggc ggcccggcga
     9301 tcattccgac ccgaatggcc acggcacaca tgtggcgggt tccgtgctgg gggacggctc
     9361 cgccgcgaat ggccgcctgc gcggcgcggc gccaaaagcg aagctcttct ttcaatcgct
     9421 gctcgacaat cgtggaggtt tggggggctt gcccttccgt ttggagactc tgttcgatga
     9481 ggcgtatcag aacggtgttc ggattcacaa taacagttgg ggcgcggcga cggcctccgt
     9541 ttaccgcgtg accagcaacg aggtggatgc ctttgtgcat aaccaccgtg atatgttgct
     9601 ggtgatctcc gccggcaacg aaggaacggc ggcggatccc gccacgggca agcgcaatgc
     9661 gagtaaagga ttcgttgact ggctgtcgat cggctctccg gcgacttcca aaaatgcact
     9721 gacggttggg gcgagccgca gccggcgcac ggcaggcggt ctttccgcgc tgaactacgg
     9781 ctccgtgtgg cccgatgcgt ttgccgaggc ccctatttcg gatgagaaag tctccgggga
     9841 catcaatgcg ctggcggcat tcagcagccg gggaccatgc gacgattacc gggtcaagcc
     9901 ggacctggtg gcaccgggga cggatattct ctccacaaaa tcttcgaggg cgccgctgcg
     9961 caacttctgg ggaccctatc cggacaatcc gcggtatgca tacatgggcg gtaccagcat
    10021 ggccgctccg cttgtatcgg gatgcgccgc ggtggtgcgc gaataccttc tgaaggaacg
    10081 aaaccacgag ccgagcgccg cgcttctgaa ggcgatcctc atcaacagca cgcgcgcgct
    10141 cagcagcccc gatgcgatgg cggaccatgc gtttctgccg aactaccatc aaggcttcgg
    10201 atgtatattc atgccctggg cgataccaaa cgaattgaac tcagggatga agctcgaatt
    10261 cttcgataat tggcgcgaca ccggctcacg gttcacacgc accggccaac ggcgccgcat
    10321 gcaattcaca ctcgaaggcg gcggcccctt gcggatcacc atggcctaca ccgatccgcc
    10381 tggccgcgct ctccagaata atttgaatct gtttatggcg gagcccggcg cgaatggccg
    10441 caagtggatg ggcaacgagc aggttcccat gagcctgaac attcccgatc ccaccaacaa
    10501 cgtggaagtg atacgaatcg acgatgcgcc ggcgggcaat tacctgattc aggtgaccgc
    10561 gtataatctg ttacaaccgc aaccgggcca ggatttcgcc ctggtggtga cagggaaatt
    10621 ggccagcccg ttggttcaga taggttgaag caatgtttag tattcagatg gtacaggccg
    10681 gattcggcga ctgctttctt gtcgagttcg gcacgcccgg ccatccccgc cacattctga
    10741 ttgacggagg tccgccggcg gtttacgagc agcatctccg gcccgtgttg aaatgtctgg
    10801 ccggcaatgg gggcgtcctc gacctggttg tgctgagcca tgttgataac gaccatgccg
    10861 taggtctact cgattatttc gctgaaatgc gcgatacgcc cggcccgaat tccggcctgc
    10921 ccgcaataag cggcctttgg cacaattcgt tttcgcgaac cattgacagg ggcagcggca
    10981 tcgaacggcg gttacaatcc ctgatgaccg cttcccgcgc gcaggtgatg tcgcaggcgg
    11041 gcaatgctgt gaacggcatc cgcgaaggcg ccgcactgcg cacggccgcg ctggcgctcg
    11101 ggatacctat caacgccgga tttgaagatg gactgattac ggtggagacg gcgcgccggc
    11161 cgttgaagct ggggaacctg ctgatcacaa tcgccacccc ggccgggctc cagcttgagg
    11221 aactccagac caaatggcgg gagtggctgg acaagcatga agaggctatc gcgaacgacg
    11281 agttcctggt gatggctaac tccgatgcaa gcgttcccaa cctcagcagc atcggcttcc
    11341 tgatggaggc ggacgggaag aaagcgctgt tcaccggtga tggaagaagc gaccatttgt
    11401 tgaagggact cgaacaggcg cggctgctct cagatgggaa gctgcatgtg gacctgctaa
    11461 aactggcaca tcacggcagc agccgcaata cgacgaaaaa attcttccgg tccgtgacgg
    11521 cagatctgta cgcggtttcc gctaacggac gggacggcaa cccggacttg gccacgctca
    11581 tctggattgt ggaagccgcc cgcgagcagg gccgcaaaat cacgattacc gcgacgaatg
    11641 cgactccttc aataaagcaa ttgaaggagg agtacaaccc agcaacttac ggctacaaac
    11701 tccagatcct gccaccggcg cggcatagca tcaagctctc gatgccgcat acagcttgaa
    11761 acggtccggg gttcgctcag gccaggtgct ctctcaactc ggcggcgatc tgttccggat
    11821 tcaaacgctc gccgctgttc agccgcgcga gaatgccatc gatcaattgc ttagcgtctt
    11881 ccttaccgaa atttttcagg aacccgtcaa agtaatgctc gcccgcgagg gcgtgattga
    11941 tttccgaatc cttattgtga tcttgcaact cggtgaaata aaccactttg tacaggccag
    12001 tgtactgcgt ctcgcggtaa atctcgaaca tagtcttttc tgagtcgaaa gcacccatat
    12061 ggcgttaact tagcacacgc ggcggccgga aggcaggtca gcgcgtccag atggcgaaag
    12121 gagtagacgt gcgggcgtca atcgtgaccg taacgttctg ccggccagag ggtacagagc
    12181 ccagacgaaa ctgcatctgg taggtaacag gctcctgcca aagccgtagc acttccgaga
    12241 cggggtgact ggttgtccct accgtcaccg tggcgcgggc cgatgcgatt tgtgttcccc
    12301 aatccgggac tccagtgaca atgacggtta acaagtcgcc aacggaagcg gggcgagtgg
    12361 cgtccggcat ggcgaaggta ccgaacaccg agagaatcga gggcggaggc gtgtcaatct
    12421 ccagcagggc gagtgcaatt ttatcaccgg cggtcaaacg cgccagggcg ggcccggcag
    12481 cgaggttagg gattcgcaca gcgatctgtc cgggagccgc cgcaaggatc gggacgctaa
    12541 catcgcctaa tgtcagcgac aaccctgctg cgatatcggc ctgagtcaga ttcgagattc
    12601 tcagcgtggc gttggagttg ggctgtagag ccgatggggt tccggacgtc gccatcaact
    12661 gaatgggcga agtgaaagcg agccgctcgg gatcggcctg ctgcaaagta acggcagccg
    12721 gctggacgaa ccatttcagt ccgctcgcaa tcgtaaggtt tgcgctcgta ccggcggcgg
    12781 cgctggcccc caccagaacc gtagccgcaa cacggccagg cgaaagcaca accactcgct
    12841 gtacgctgac atcggcggat ccgaagccgg cctgggcttg cccctgcgta aacatcgcac
    12901 ccggagcacc gatctcgatt acggactccg ctcctaccgg gagccggtcg ggagtgatcg
    12961 ttacgttgga gatgccctcg agacccacat cataactgta ggtgggaggg gcattgagct
    13021 gcgtaaacat cgaggtctgc ccgtcggaat tcagagctgt gaccaccgcg acatgaccgg
    13081 cgggagccgg cggcgcaaca actacggcgt tcgactccgc ggtttgcaca agtgccgccg
    13141 gctggccatc gaaccagtat cttgtatcgg gtgcgagatt ggcaccggat aacgccagcg
    13201 tgcgattctg cggcccgccc ggggccaggg atatcgcatc gatggaaggc ggcccttttt
    13261 gtactaccgt aaacgcactc gggaggacgt aaatggaacc atcgagcgag aagacgatgt
    13321 gacgcgggcc gtccagcagt tgggaaccgc tctgaaggtc gatgaccaga aactccgaag
    13381 taaacggcct caagctggta acggatacgc cgccgccgat gacgctagcc gtcaatcctt
    13441 ccgtaggacg ggtcgccgtc ccggaaccgg acaaaatccc cgcacccgca gccacgataa
    13501 acccctgttg cgaggtgaag ttcacaaacg cacccttaac gccgtattcg ccagggtagc
    13561 tgtacgtttg tacggaatga atgaagggcg cgctacggcg ctgaacggaa aagttcaagc
    13621 cttccgtgat ggtgcctgct gcaacactca ctaaccccgc ctgttgccat tctcttgtcc
    13681 cgggaaagaa ctgcgtctcg aagttgggtc ccgccggtac gggttcctga tccggccccc
    13741 tcggtaaaac gatgttcgcg gcgctgactt cagcagccag ggcgggcggc agcggatgag
    13801 tgtatagata gtaatcgcct ggaggcagcc cttcgatacg gtacgtgccg tccggattcg
    13861 tcaacgtgct gatggcctga ccccgtggcg gcagggctac aacggaagct aatccgacac
    13921 cgtcgccggc cagtaccacg cgcccggtga tgacgcccgt cgcggattgg aattgaggct
    13981 tggggtagag cagggagatt gcggcaacat cgtcagccgc cagaggccgt gctttcgaag
    14041 tcgcccgggt aatttcggtg gacatcaccg aggacgtgag cgtatgctgc aaacccaacg
    14101 tatgtccgaa ttcatgtaca gcggaaaggt agaatgcgtc cccataactg ggacgttggg
    14161 agagatcccg gttaaagacc agcagggagc gtacgatagg aacaaaacta cgtccattcg
    14221 catcctctac cagatcggca cgcttggtcg gtccacccag agcaatcagc ccgggaggga
    14281 tttcgtccga gaaaattaca tcaataccgg gcccattcca ggccgtccct tccggagaga
    14341 caccgccgaa agccagactc aggtctgacg actcgatgtt ccaggctgtg gcggcggcgc
    14401 gaacctgact gatgacggat gtcaggctat cgccgggagc aagtttggaa ggaccgtttg
    14461 ccgagatgta atactggatg gttttgccgg gcagggaagc gagatcgaac ttctcgtaaa
    14521 tcgggaggaa gggcgcggtt tttgtatcgt aatgaacgaa atggtagtag ccggaggcga
    14581 tgccggacgc cacgatgagc gtggctacgg gagcaaacag gcggaaagcg aaccgtttca
    14641 ctgaagcgcc tccgctcccg cggcactccg gaccctctgg cgcagctcat cgagagaaag
    14701 cgtcagcccc tcgtcccgta ccggctttcc cgaacggaca tcgagcatcg gttcggagat
    14761 cgccatgcgt gtaaccactg cggcgccttt ggaatcgtcc tttacggcaa gagcaccctg
    14821 cgacaggccg acaaggtggt ggagtccact cggagaccgc cagacaaaca agacatattg
    14881 cgatcccttt aagagcaaag gagagccgga aaatgtctgc tcgagggcgt tcgcccggcc
    14941 gccgggtgtg acgacctcga tggagccagc ggcactgccc ttcagttctt cctgaacctg
    15001 aattttccag tgcgtataaa tgagcggtcc gcgaaaggcg gcataagacc cggaaacggt
    15061 gcctcgaacg acgatagtgg atttctgaat catctggtcc acactcaact tctcgagagt
    15121 ggcggtagat gaaactgtcg ctgccagaag gagggcaacg atcaggcgga atcggaaggg
    15181 catggtagtg gcaatcttca tggagcatcc tgatcgccaa acctcaaata catggaaaag
    15241 cggggattga ctttacataa ccgcttatgt ctgtatacac gggacacgca ttgcggggca
    15301 ctctgggacg ctcttacttt tcgtcccgga cgatcagaaa aggcacactc aaatcatgac
    15361 gaactgagtt aattgttgtc ccgaaaacaa tgtccttcaa gcctttatgt ccatgggcgc
    15421 ccatcacgac gagatcgggc tggagggact ttgcatattt cacaatttcc atcgcggggt
    15481 tgttggagtg aaggatgacg gcttccgcgt tgatattacg ctcccgaagc gacctggtga
    15541 gacgtccgag gtaggctgcg ccttcacgca cttccaccgt ggaggataat tcgccgtaga
    15601 cctgacttgt gacgccttcc tccacatgaa tcaggtacag cgttgcgccg tgcgtttgcg
    15661 ccagggcggc ggcatgggag atcgcatcgc ggtcccggtc cgagtggtcg acgggcacga
    15721 gaattttccg gtaggccggc agtgtcagcg cgccggaggt atccggaatc gccaccggcg
    15781 cacggccgag ttgacgcagc caggcaggca ggaagggctc gaagaccacc catagaagca
    15841 aagcaaccag gccggcgccc aacaatacaa gcaggactcc gaccggtacg gcgtatgagc
    15901 ccgactcgcc gatccattcg gcgaacgtat cgtataccag cttcaaattc agtgacacga
    15961 tgatggtggc taccgtccac gccagggccc tgacccagcg ggggctgacg aactctccca
    16021 tccgccgccg gtcgctggtg aaatgaacca gcggaatcac ggcaaagggc aattgcatgc
    16081 tgagaatcac ctggctcaga atcagtagcc ggaacgtgcc ctcttcaccg gaaatgtaga
    16141 tggtgatggc ggccggaatg accgcgatca tgcgcgtgat gagccgacgg agccacgggc
    16201 gcatgcggat gttcaggaac ccctccatga cgatctggcc ggccatggtt ccggtaaggg
    16261 tcgaggattg gcccgccgca agcagcgcca cggcaaacaa aacactcgcc acggcggttc
    16321 ccagcagagg agccagcaat agatgagcct gatcgatttc cgttactacg atgccgcgct
    16381 tgaaaaatac cgacgccgcg agaatgagaa tggcagcgtt taccagcaag gcggcgttga
    16441 gcgccactac cgaatcgatc agattgaacc ggcaggcggc gcgtttttca ctcgttgtgc
    16501 tcccgatccg ccgcgtctgc accaatgccg aatgaaggta gagattgtgc ggcatcacgg
    16561 tagcgcccag cattccgatg gctacataga ggctatcgcg attcagccgc gggacaaggc
    16621 cggcggccat ttccgtgaac gacggcttgg ccaggatcat ttgcaccaga aagcagccgc
    16681 cgataacggt gaccagtgca aggatgaaag cttcgatcat ccggattcca aaacgcgtga
    16741 accaaaggat gaacagcgtg tccgcggcgg tgatgatcac ccccgccagc agaggcagtc
    16801 caaagagcag attcagcccg atggccgctc cgagcacctc cgccaaatca caagcggcga
    16861 tcgcaatctc gcaaaagaac caaagcacca gcgcgatggg gcggggataa gcttcgcggc
    16921 acgcttgcgc cagatcgcga tgggccacga tgccgagacg cgcgctgagc gtttggagca
    16981 gaatcgccat caggttcgac atcaccagca cccatagaag ctggtaaccg aagcgtgctc
    17041 caccttccag atccgtagcc caattgccgg ggtccatgta ccccacgctg accagatagg
    17101 cgggcccggc aaaggcgaac atccgcttcc agatggaaag ctgcccggtg ggaactgagc
    17161 cgtgaacttc cgccagagag cgtgaactgt cagccgagtt gggggtaatc atggattcgg
    17221 cagtagtgtt aactgtagtt tagtatgatt gtaccgtggg cttcgcgtca atatgtctta
    17281 cgggctttcg taccagggtg agtagcattg cggccagcag accgctggca ctcatggcgg
    17341 caccggcgaa caccgaagca gggcggtagc gaaacgtgac ggtgtgcgtg ccttccggga
    17401 cggcaaccgc ccggatggcc ccgtaggcat cataaatgtg agccggttct tcatccaccc
    17461 acgccttcca tcctgggaag taggtatcag ccagtacaag caggcccgca cacttcatgt
    17521 ccgccgtgat atcgaccgaa cccggtaccc gccgcgtaat gcggaaccgg tcctccccgg
    17581 catcgcaccg ggcaatttcc ggagggggtt cccgtaaaat ggctgtcgtg cgaagatcca
    17641 agccatcttg tcgaaccagg tcggtgatgg ctccgtcgcc ggctgtcacg atgttatgga
    17701 cgcttcttac aagagggtag gcggaaggat taaagtaaac cttgagcttt gatgccggtg
    17761 agtagtaaac ttccacggcg ccttcgcgga gagcacctgg gcgtggtgga tcatttacga
    17821 ccgaatagtt aacggacagc agatcccgga agcgggcgaa gtagaattcg tgcgctacga
    17881 gattggtggt gatgctcgcc agatagccgc cgctttgttc gatgccgtac caatctccga
    17941 aattgtaggg tatgtccgcg tctgcgactt cgactcttaa aggcctgggc tgtttttgca
    18001 aaaaaccagc cacatcagcg gattgggcta tcttcttcaa gtaaattgta cgatcttttt
    18061 cctccgtaga tacgaaggaa taagaagtgc cgttgccgat gtcggcaagt gccacggcaa
    18121 taatggcaac ggcgagcgca ctttcgccca gcttgccggt tcggcgcgcg gaaatgatgg
    18181 cgctcatcag gactgcaacc aggccggaca ggatccatcg agtgtcgccg gccagtttgt
    18241 aagtggctga caatgccgtg gccagcgcga gcaccgacaa tccgaatacg gccagagcga
    18301 tggcaaccct ccggctccac agcgaatcgg gattcagcaa agtatcgaat ccataagcga
    18361 caaggaccgc cgatgcaaag tgaaacacga ggatcgccat ggatggggat cgcgccttct
    18421 cgaccattgg aaccaatgaa tagagaaggc catgaagcag ggaatagccg ccaagggcag
    18481 tgaacaatcc cgctatcgca acaatcgtga agatccgcac ttcgaggata cgccacaacg
    18541 ctgccgcgcc cagtactgcg agcgaaagcg ctaccacgcc ccagaacacg tcggtgttgt
    18601 ggctacggcc cggtaaaatc agcccggtca aagtgtccgg cgtgaccgaa taggtcttat
    18661 gtacggtgta aggtaccttg tcgttccaac cgacgggctc cgagcttccc acccatcgct
    18721 tggcggcctt accgtattcc agcgcgggca gcgtttgaag gccgctggcg aggataaaca
    18781 caaccgtgaa caacgctccg gcagccatcg tccggcggcg ttcgtatacg cgcaccagca
    18841 acccgtacag ccaaacgcca gccgaagcga gcgtcaggaa aatgggaacc tggtggtgtc
    18901 cgctgagcca cgcgacgccg aggaaggctc cgctggcggc ggcgctcgca taaacgcgtt
    18961 caccacgaag acagcgaagc tggtagagaa acaccagcgg cgcccagatg gcaccattca
    19021 acatctgggg ccaatcggtg gtaccgatat atcccccgag cgcaaaggcg catccggcaa
    19081 cgatggaccc tggacggctg cgtcccagat cccggcaaag ccaataacag aacagcgcag
    19141 ccaggtaatg aatcaggacg aaataccagt tcagatggct ttgccgcagc caaccgttgc
    19201 ggagcgaggt cagaaaaaac agccagttcg gcgggtaagc cgctccgggt tgtccttgtc
    19261 caatcagggg ctgaccatac cactgatagg ggtcccacat cggaaaccgc ccggcgtgcc
    19321 attcgccagc ctggaactgg taccagggaa gcacctggtt ggcaagatcg gggctctcga
    19381 gccatgtgta ctcgttggtg agagtgagtt tccagtagaa gccggcgacg atcgcgaaca
    19441 gcagcaggac cggtgtgatg gagatcagcc agcggcgtgg cttaatgcga tcagtgtggt
    19501 ggatttcggc cacctgcatt cagagtcatc ataacggaac tccatcagga tacaaaggtt
    19561 tcctctttgg cctccggttt gcaatcgagt aatacgaaag tgaacgggag gccagtcatg
    19621 agagccactt atcggatgtt gttactgatg atggccggcc tggcgagtta cacgtgggcc
    19681 gacgaagacg aaggtccggg acgcggcgtc gcccggatca gtgtcatcaa cggcgaagtc
    19741 tcagtacgcc ggggtgattc cggcgacctg gtggccgcga caatcaatgc accattggtt
    19801 gtacaggaca cggttctgag cggccgttcc tcgagggccg aagtgcagtt cgactatgcc
    19861 aacatgattc gtctggcatc gatgacggaa attcgcctgg cggaactgga gtacgaacgc
    19921 tatctggttc aagtggtgca gggtaccgtc acattccgcg tgttaagaga cacgggcgca
    19981 gagacggaga tcagtactcc gagtgtttcc gtacgcccaa tcaagaaggg ggcttaccgc
    20041 gtaacggtac agccagacgg ctcatcggaa atcaccgtac ggtccggcga ggcggagatc
    20101 tatacccctc gcggcaccga acgtttgcat tccgggcgaa ccatgatggc gcgcggttcg
    20161 ccgtccgatc ccgagttcac cgtggcaaac gatattccgc acgacgattg ggatcgttgg
    20221 aacgagagcc gcgaccggga cctggagcgg gcccgaagct atcaatatgt aagccgtgac
    20281 gtctatggcg ccgaagacct ggacggtcat ggccgttggg tgagcgtggc gccctatggc
    20341 tgggtctggt cgcctcgagt ggcggtgggt tgggcgcctt accgtcatgg ccgctgggtc
    20401 tggattgact ggtacggctg gacgtgggtc agccacgatc cctggggctg ggcgccttat
    20461 cattacgggc gctggttcca cagcgggtcc tacggctggt gctggtatcc cggccagatc
    20521 cgtgcccggc attactggcg tcccgctttg gtcgcgtttt ttggatgggg acgccacagc
    20581 ggagtgcata tcggcgtggg tttcggctcc gtcgggtggg ttcctctcgc cccttatgag
    20641 ccctattacc cctggtatgg gcgcggctac tatcgcggtt atcgggaccg ggtttatgtc
    20701 gataacagcg tacgtgtcgt aaacaacatc aatattacca acgtgtaccg gaatgcacgc
    20761 gtgcgcaacg gctacacggc ggtggatgcg tatgacttca cacaagggcg cgcgggccgg
    20821 tatgttgaca ccgccggcgt cgacctacgg cgggcgaacc tggtaaaagg ccagattccg
    20881 gttgcgcctt cccgcgacag cttgcgattg tccgaccgcg aggtgcgggc gggcaatttg
    20941 ccaccgagcc gggcgggcga ccggtttttc agccggcgaa cgccccaaac catcgagcgc
    21001 gtctcgttcg aggaccagcg gcgcagcatg gaagaagtcg ctcgccgtac cgtacctgac
    21061 cggggaggag cgcctggtgg cactcggagt gaagcggcgg caggtgcacg gccgggggca
    21121 accggctttg tccaggggcg tgccggccaa gtcgaaacag gacggacagg aaatactacc
    21181 ggttggcggt ctgtcagcgg ctcgggcggt actgaacgtt cggacacgca ggcgcgaccc
    21241 ggcaatgcga atgaagggtg gcgcaggttc ggggagccgg cggggcccgg aagcacccgc
    21301 gctgagcggt ccgcagcgca acccgggcgg accggacagg atggctatcg ccagatcggg
    21361 gaagccggtg gccggacgga agcagatgga tggagacgtt ttgggaatcc ggcaaccggg
    21421 aaccggtcgt cgaatccggg agtgcggcaa gccgagcccg atgccagccg tgggcgtgtg
    21481 gaacgaacgg aaggggcatc ggcaccggca cgcagcgata cgagcaaccg gcggaggttt
    21541 gagagtggtg ccggctcagc ggcacgcggt ggcggcggcg gaacagaaac cagcggccgg
    21601 gttagccgtt ccgattcgac cggcggggct cgttctgaac gatccgagcc cagccgtgtg
    21661 acgccgcgca atgaagggtc gcgtggcggc agccggttga gcgcgagcga accgatccgt
    21721 atcaatccgc ccattgtccg ggagcgagcg ccttcgcgat ctgacggcgg ctcgcgcgtg
    21781 gggcgcagcg acatgccgcg cggcggcagc agcggcggag caagagtgga gcgcagcacc
    21841 ggtggaggag gaggtggccg cagcagcggc ggtgccagcc gaagcagctc tggcggcggc
    21901 ggaggcggac gagaatcgcg aggagggcgc gggcgctaaa aacggtttcg tctgactcca
    21961 tcctgaaagt tttgtttttc ttccctcaac ctggcctgtc cgtgaaagcg ggcaggccac
    22021 tttttttagt gctagacctg gccgacagaa aaagttaaca taatatccgt tatcagaact
    22081 tgaatccggc gccggaagac gccctccagc gcctggctga gatcgccccg acgctgaagg
    22141 aggccgagct ccgggtgctg attgagctcg tcaatcgagc cctcagcgtg ccatcccgag
    22201 ccgtccgcgc ttccagccgc gagctcgcaa acgccacaaa aaccgcacgt tccaaggttc
    22261 agccagcgct agacgctctc acggaacgcg gccttattac cacacgccaa ggcatgccca
    22321 cgcgggcggc agtccatcag atcaatctgc tactcaccgt ccggatgagt ggccctgtaa
    22381 caggaccacc ccctctcgca ggtggccccc gaaaggggcc accccaagac agcacctggc
    22441 ccccgaaagg ggccaccctg gctctcttcg gggaccactc tggcccctta gaggggccac
    22501 cccccacaga aaacacgcca cttgcgcctg accccgccgc cctcgatata tcgataggaa
    22561 ctaccgatgt aatcgatcga atgctccggg caacgaccaa gaacaccccc gatcccgacc
    22621 tcatccccat ggcccgccaa tggctccacg gctacatggt caaacttggc cgcgaaaagg
    22681 accgccaccc gccggatgat cgcctggtgg cacagttcct cagcatcgcc ccctggccac
    22741 agctcgagcg gctcctctat gacctgctgg ccgaacaccg cgagccgggg taccagtacg
    22801 cctggtttgt caccgtcgcg ctccagcgga tccacggcat tcagcccaaa cagctccagc
    22861 agcgccgcgc catgctgaag ctcatcaaac ctgccgctga gcaagccgcc aaggaaggcc
    22921 aggaatgggc acagcttctc atcgggcagg tcgcctccaa taaaaccctc aagtaacacc
    22981 tgaagcgcgc cgaagagccc cacatggacc cttttgccct catccagcag ttgcaccatc
    23041 agctcgacct gctcacccgc gccattcacc agccacccgg caccgccatc tctgttggcg
    23101 acctggtgca actcgccccg gaatcccatc ccgtatacgg cggctatctc atggacgtcc
    23161 gcaacgtatc cgagacccgc gccggcggcc caatcctcgc cacctaccgc ggcgcctgga
    23221 cctccatccc tctcgaccgg ctcgtccgcg tgtcggcgct ccgcgccgcc gcgatcgcgc
    23281 ggcgcaaagc gcacgaagcc cgcaaagctt gaccacaacg ccacaattga agggagggag
    23341 agtcggtatg gcgcctgaaa tagagctcct caccatcggc gatctgcacg tgcgcctggt
    23401 gcattacatg cgggcgctcg tacggcacgg cttcctgtcg gaagccggcc tcgcccgccg
    23461 cacgcatctc agccagccgc acattcacca cgtgctagcc ggccgccgcg ccctgacggc
    23521 cgcatccgcc gacctgatca tcgagagcct gggcctgggg attctcgacc tggtgaccac
    23581 cgccgagctt gccgcttacc tcgacgagct cacgcgacgc gttccagccg cggacgtccc
    23641 gcccgccgtg cagtagcgca ccgctcggcc acgggacaca gcggcagcag ccgcgccccc
    23701 gtcgccctct ggtaccgctc cagtgcctca atcaggcgct ctacggccat ccgccgctcc
    23761 atcaggctct gcaactgctc gtcaatcgtc gtcacagtat gcagtgtgcc agcacaccgc
    23821 gccctctcga cagtccccca atgcggttcc acgcccacaa tgatgcggct gcctccgcgt
    23881 acactggtac tggctccctt cgagcctcat gcacgcaccc ggccggcgct tcacaaccgc
    23941 atgtcagcgc cggccacgcg ccgcttgctc cgccgcgtcg ccgtcgtagt caatccccaa
    24001 cagcccgccc gcgatagcca tgacacgccc aacgcgcaca ccgcagcacg tcacaacgcc
    24061 ataatcgcca ggcgtgcggg tcgcacgctc ccgcgtgcgc cacaccgccg gcacttcgcc
    24121 ggtggcgtcc gaaataacct cgacatccac cgccaggtgc tcgccggccg ccagcggccg
    24181 ctcgagcaat tcctcttgct ctgtccagac aatgccctgt gcctcgaccg cggacgcccg
    24241 cttcgcgcgc gccgcttcca tctgctttac cttgccgacc agtcgcatgt ctccatcatc
    24301 ccttcagcag ccggtccagc tcatcccgca gcccgccatc gtccaccccg gcccgctcca
    24361 gcgcttcaat ccgcaccgcc aggtccgccg tttcgatggc tcggcgctcc gcttcgatca
    24421 gcgccatgaa tccgcgggcc tcttcgagcg acaactgccc cgcggcggcc gccgccacca
    24481 ctaccgaagc cgcttgcacc aggtcctccg ccttccggat cttgggtagc tccagcgctt
    24541 ccaccacgcg atcacggcgc accggcaata cgcgctcgat caccaggcgc agcgccgccc
    24601 ggtctccgcg cttcgccagg cctaccagcc tcgccgtgat ggcttccgcc gatccgtcca
    24661 gcaactcctg gcacagcagc gaggcgcggt tccgcgctcc gcgcggccgc cccgccgggt
    24721 tgccgctcgc gccaggcgtg aaccgccccg cagcagtgcg tttcgccgtg ccctcttgtc
    24781 tcgcttttgt tttgtcagcc atcgtatcca cccttctaag ggaaacacct tagccgcacc
    24841 ggattcggtg ctcgcgcccg ctcgatcatc cgttaggcga tccaccgccg cgaacgaccc
    24901 caccacgccc cccacgcgca gccccgagct ccggacgccg gggcgcggcc gtgcattggc
    24961 gcacacaacg cgccggcacg tccgcgcgcc gcacagcgat ccacggccgc cagcgacgca
    25021 gcaggcgcac agtggacgtg atagcgcggc gcttcgtgcc ctggtggcac tcgcaccggc
    25081 ccacggccgt gcgcaaggga agcaatcacg tttcggagct cgctccgctc gattttttag
    25141 gcgatcggcg agcgtgcgcg gtcaacataa cgccaaagtc attatcggca accgcggcca
    25201 accgaaagcg gaccataaag cgaaaggcgg ttccgataat ggcgcgttca tgttaaccgc
    25261 agcggcatcg cgcgcgcagc cccgcgccac aacacaggac ctcccgcgcg ggcgctcgcg
    25321 accgcttcgc gctcccggcg ctgcattgcc cgtgcgcggc gccccggcga tcctccaaca
    25381 cagcacatca agcgcgccgc tcgcgaagct cctgcatgcc cgatccgcgg gcccggcgcc
    25441 cgctccaccg cgcaaggatc caccgaagcg catcacagca cacgcgcgga ttcacccggg
    25501 ccacggcgct cgcggccgct tcgcgcttcg cggccctccc acaccccgtg acgctcgcgc
    25561 ggccgccagt gtggcttcgc ggccgcatgt gccgctcgct taactacggg attcggttgc
    25621 gcttcgcgct cttcgctttt aatcaccccc agcccgcccc ggcgcgctgc cccccggctc
    25681 gcttcgctca ccaccccccg cccagcccac gcaaactgcg cgtgggtccc gcggggggtt
    25741 cccatccggc cccctcaagg gggcctccct ggcgcgccag gcgcgcccaa agccgcccat
    25801 cgcgcatgcc cgcagcccaa cacaccacca ccgcgccagg cgaacgcaaa ccgcgcgttc
    25861 gcttggcact catcccgtgc gcggcgccta cccgccgtgc atcacagcac ctcgagcccg
    25921 cccgcttcgc gcgcgccgca cgatcgcgca ctaccgcacc gcgcgcgccg cagcactcca
    25981 gccgcggcgg caagcgctca cagccgctcg cgcccgccgc tccagcattc cgtgacgctc
    26041 gcgcagcagc aattgtggct ccgcggccgc gcatgccgct cgctctgcgg cgaaagggca
    26101 acggccaacc gcgggccgcc gcccgtcatt ccgccggctg ccaccgcttc gcggcccatt
    26161 cgcataaact ccatgcgatg gggcccaggc agccggccaa ggttgcgcaa gcgcaccatt
    26221 cctgccggct tttttcggct gacccgccag cacaacccgg cgattcggct gcgttggcgt
    26281 ccctgtaaag cggcccagcc acacaaaatt tcaaccgctc aaattttgtg tgcaaggtgg
    26341 cttcgccgct tcatttgacg ccgcttgaca gggccttcgc cgcagccgtt acaactcgcc
    26401 agtgctggcg ggtcagccga aaaaacccgc cggtcagccc ccgctacgcg ggctgaatct
    26461 ctcaacccga aaggtgcaca atgaacacgc aacgcgagaa agtgcagttc acaccaggcg
    26521 agacagtcat cctcgcgctc gaattcgatc caccagccga tccccggcca ggccgcttcg
    26581 gagatcagta catgtactgg ttcgccggtg acaaggtcgc ctggctcgat ccgcccgtcc
    26641 acgcgcagat cgtagccacc ggcgcgcgcg ccggtgacga aatcgctatc acgcgcgccg
    26701 aggtccgcaa cggcgccagg aagcgcgtcg aatggcaagt acagcgcgta gaggacgaac
    26761 ccgccgacgc ctacacgcgc cagctcccgc ccgaacaccg gcccacgccg cccaccgcgc
    26821 ccgcgcaacc cgcggccgaa ccgcgccagg tcgcaccacc gcggcccacc gcggagctcg
    26881 catcggccct catcgcggct atcgatgccg cgggcgtagc cgagcgctac gcccgcgagc
    26941 agggcctcgc gatccgcttc accggcgaag atatccgtgc cctggccaac gccatcttca
    27001 cccgccccga aaggagcctc tgacccaccc ccgtaaattg tctgcggttt gctatcttgg
    27061 aggtgtcgcg ccccctgagc gcacctcgcg ccacacgagg atagccacca gatgaccaac
    27121 gaacagctcg cctccatcaa ccagcgcttc gacgacatgc gcgacctctg gcgcgccgag
    27181 ctccgccgcc tcgaagaggt cctcgacgct cgcatcaagc aactggacgc tcgcctcaag
    27241 cacctggagg ggcgcatatg accatcgacg aacgccttga agccctcacc cagaccgtcg
    27301 agatcatcgc cggaatgcag caggaaaccg aacggctcat gcaggagagc gaccgccgca
    27361 ccgaagcccg catagcccaa ctcacggaga ccatgaaccg cctcgccaac atcgtcatcc
    27421 gccacgaaga acgcctcgat cagctcgacg gaggcgaccc ccaatgaccg atcaattcat
    27481 catcgccatt gccgtcgcac ccgtcctctc cctcgtcatt gtcgtcgccg gttacatcgt
    27541 ccaaaacagc aacctcaacg cccgcatggc cgaattcgcc atccgcgttg gtgacacccg
    27601 ctccgagctc cgctccgccc tcgaacagga ccgttctgcc ctccgcgaat tgctccgcgc
    27661 cgaactctcc gccgttcgcg ccgaaatggc cagaaaccaa agcgagcttc tctccaagtt
    27721 cgccgagctc gatcaccgcc tctccacgct cgaatcccgc ttccgcatcg agcgccccta
    27781 acatggaaga ctcgtcatga cctcacgctg gcgtaactac gcagctccaa tcatccgccg
    27841 cgtgctcgca gacaccgccg gccgtccgga agcggaagtc cgcgcagcgt tacgagcggc
    27901 ctatcccttc ggtccacgcc ggaatcaccc ctacaaaatt tggctcgacg aaatcgccgc
    27961 ccagaccggc cgccagcgcc gcaagcggcg acacccttcg gacccgcagc agacgttcct
    28021 gtcgttcctg taaatcccca gttctttcag gcgagaatgg ggacgtgaag aaaagcaaac
    28081 ccgcccctaa acccgccgca cctgccaagc acccaccgcc ccgtcaccgc gacgcgagcg
    28141 gccgcttcaa gcgctccccc gcctccaagt gatgcagagt gatgcagagt gatgcagacg
    28201 tgggacgagc acgcacccaa tcacgccgcc atccgccggc gtttcaccgc cagcgcagat
    28261 agtcgaagct ccagcggtag ttctggctgt actctttgcc cgaccacgaa atctcatcct
    28321 ccagcgccca cccccacaac tcatcacccc gccgaatgta ctcccgatca cccgtcaccg
    28381 cccaatacca gccatacgca ggcaaaatca gattgagcca cctgcgcccg tacgactggc
    28441 aactgttcgg gcaccgcgcg cctggcggtt cggggttgta caccattcgc cctgcctcac
    28501 tgtcccagcc gtgctcccac agccagtcca gcatcgagcg caccgcaggc accacccgcg
    28561 ggtccccgct gtgctcgtac cacgcaatca acgcctccgc tgccaacccg tcgaagaacg
    28621 tctggtgcaa ccggaatgaa cccgtgacga acaaccggtc gaagtgcccg agcagataat
    28681 ccaccgcgcg tgccagctcc ggacgccgcg gctcgcccaa ccgctccgcc gcgatccacg
    28741 cctgcaccat atacgccgtc tcccggatgt actcgtcgtt cggatcgccc gcgcgcgccg
    28801 cgaatgtcga gttccgcgcc atcagcagcg ccgcttgtct gtagctatcg tctccggagc
    28861 gctcataggc catcaccaag ccgtgcggga acacccgcca gccctgtatg ccgccgttcc
    28921 ggcttagcag gttgtcgcgg tattgcgccg cgatcgcgaa cgcgcaggcc tcccagctct
    28981 tatcgcccgt ccaatcagca agctggaagt aaacccgcgc cccgtcgtaa taccagacct
    29041 cgctctcatt tccaaaccca tacgtccgcc cgtcgcacca cttccggccc aggctcaccg
    29101 ccaggctctc ccacctggtg atatccagcg gcggcaacgc gccgcgcagc gacaccgcac
    29161 ggtacggaat ccgcagctcc accgcctcgc ctggcgccgt agccactccg ccgcctgccg
    29221 acggctgtaa tcgcatcgcg atcaccgcct cgcgccgccc gggctccgcg cccgcttcca
    29281 cttccaccag gaacgccgag ccaggctgca cgttgtacac gaagctgcgc ccgtctttcg
    29341 cgcaccggtg cagcggcgcc aggtgcgcgt agctcatcgc cacgccagcc ggcatctgct
    29401 cgacgtacag gtggctgaag ctcaccggcg ccgcgtccat gcggacgatc actttcgccc
    29461 gcagcttcga ccctgcaaat acattcgacg gcgcgatcac ctcgagcctc gtttcagcgg
    29521 ccgcgcacac cgcggccgcg ctcagcaata tcagtgcttt cagtgctctc atttcatgac
    29581 tccagacaaa ttcaccgcgt caccacgaaa tcttccagcg ggcaggtatt cgcgctcgag
    29641 ctcgcgtgcc ggtcggcacg cacggtcagg acgatggtgt gcatcccgag cggcaaatcc
    29701 tgcacctccc acaccacaac tcccggcgtt gccgtggcgg aataaagctc gaccatctcg
    29761 gcggttccac catcaagcgc cacgtcgaac cgcccgcgcg ccgaacttcg cgacgtgacc
    29821 acctgcacac ccgtgcccca gaaacggtac gtcgccgtcg cacccgcact cgacgagtaa
    29881 atcccatagc cgttcgtggg atagctgccg ggaaagtaac tggtcgtcgg ccacgaaccc
    29941 gtcagatcca catgcgcggt gttcgaccgc cccgtcagac cttccggctc cgtccccgcc
    30001 agtgtgtgtg cgtactccca catcgccgta gcgtcctgat accggtccgt gctgcccaga
    30061 atggccacga tgtaagcgtt gccgcccatc tgatgcagcg ccatcaggca ctgccccgcc
    30121 tgcactgtcg tgcctgtctt gccgctgatc gtccccggat actggcccag cgcccggttc
    30181 gtgttcttcc agttgtgcgg attcgccggt cccccgctca ccgccgtccc cgcgtacgac
    30241 aacgtcgccg agatgctccg cagcgccggc acgcccaggt acacccgccc caatgccgcc
    30301 agctcccgcg ccgttgtgta atgccccgcc gcgtccatcc cgtgcggcgt cgcatacgcc
    30361 gtgtggccca ggcccaactc cccggccctg ctgttcatcc gcgcgatgaa tgccgcatac
    30421 cccgtagctc catccgtgcc gcccaggtag gtccgcccca cgtgctccgc gatcgccacc
    30481 gccgcatcgt tgcccgatgg cagcatcagc ccccgcagca gctccgacat cgcgatcact
    30541 tctccggtca ccagttccgc ccgcgtccca accgtcgccg ccgctgtagc cgaaaccgtc
    30601 acctggtcgt ccagttccgg gtcgtattca tgcgccaaca acgccgtcat gagtttcgtg
    30661 gtcgacgcct gcttcaactc cacgtccggc gctttactcc agacaacctg cccggtctcg
    30721 tagtccagga tgaccgccgc ggccgccgat accgtcggct ccggcaaggc ccagccctcg
    30781 ccgctgaaca acgtccacgt atcgcgctca gtgcagccgt aaatactggt cgaacttgtc
    30841 tgcaggaaca gctcgccaac ctggcacgtc aacggcggtg agcccggccc caccttggct
    30901 ccacccgtgc cgctgaacca cggcaccgtc ttttgcgcgc acaacggcag cgccagcatc
    30961 acaatcagca ggaagcgctt catcgcgtga acgccgcccc ccggatctga tcgccatccg
    31021 tctcgcgcag cacgtacacc gcgcccacgt ccagcacgtt gccgtagctc tcatctccga
    31081 gccactccgt atagcccggc ggcagctcgc cccacgccgt ctcaggcgtc atgtccgccg
    31141 cgtcgcccag gtagatccgg cccgagtttg tcaccggcgc ctggatccgg atcttgttca
    31201 ccccgcgcgt cccgtccacg cacacgagcg cctcggcgcc cgcggcgaac tgcgggcgca
    31261 ccaatcgatg tatcgccatc gttcttcctt gttctgtttt ggaattgacc tacaaaaggg
    31321 aaagggcctc agcgagcaat actgaggccc tcaggtacaa gcacgaccag tgcccggcat
    31381 ggcagcccgg cccggctact actcccattg tcccaggccc caacaaagca aaagcccccg
    31441 cggggtgcac caacccaccc ccgcgagggc ttccacaaag gaccaatccg gcggtgtcca
    31501 ccagcccagc gctgccacca gctaacgcag accgcaccgc cgaatccggc taccactcca
    31561 attgtcggct gttcagagcg cgcgcgccag aggattcacc cggaaaagat ccgcgatccg
    31621 tacgtaccgg cccatcactg cccgcgaccg gtgccgcgat cggcgcatga ccaccgcctc
    31681 cggtaccccg tccacgatcg ccgccgtcac catgcccgcc cgcaagctgt gcgccccgta
    31741 ctccgccgga tccagcccga tcagcgccac acagcgcttc acgatgtcga gaatcgaccc
    31801 cgcacccagg ccacggctcc gatccagcct ctcaccacgt ggcgtgaacg gatgaaacag
    31861 cggtccaggc gccgtcccgc gcaccttcag ccaccgccgc agcatccgca ccggacacgt
    31921 gcgcgcgcgc cgcccgggaa agatccccag gctcaccccg tcgccctcct ggtcggtctt
    31981 gctccgcccc acatgcacca ccaggccctg ctcgacgaat cgcacatccg agagctccag
    32041 cgccgccagt tcgctccgcc tcaaccccgt ggcgaacccc aatacaagta ccgcccggtc
    32101 ccgtacgtcc cgcgcagtcc accgcagcgc cttcgaggcc ttgaacagtt cctctggcgt
    32161 cagcgccgcc ttccctgccg gaccgctgtt cttccgccgc cgtttgatcg ctccgagcag
    32221 ctcgcgagtc tcctcgctca ccgggctctt gtgccccgcc gcctcatgcc tggcacggat
    32281 cgccgccagc cgcagctcca gcgtcgccgg cttgtacacc ttcgaccggt ccaccaggta
    32341 cagctccacc gcctcgcccg acgccggcag cggccgccgc cccgcctccc ggcaccaccc
    32401 ctcgaaatcc gcccaggcga agccgtacgc ccgaaccgaa ttcggcgccc gcttcgcggc
    32461 cgccagttct tcgcgttccc gcctcaaccg cgccatatcg atccgtccat tcagctccag
    32521 ctcaggaaat aggcaaagtt gctcaagttg ctctggtggc atggaagggg ccgtcccgtg
    32581 gccaggacct cgacacacca gaatacctga agacaacaga tacttgtctg cgatatgcga
    32641 tatgctcaat ccaagcgccc gccaggcgga cgcagtcgcc cactgccacg tgggaagccc
    32701 gccgcggccg cccacgtatg cacgcgcgaa gcgcgtagcc cacagccagg gagattcaca
    32761 ttgtcggcca ccagcgacgc accagaccca caccccatcc ccaggcaaac tcaccagact
    32821 atccgttatc agaacttgaa tccggcctca aacacatcga gcgcccgtaa ccccggcacg
    32881 cgccagtttc cgccggcacc cccgtaaatt gtctgcggtt tgctatcttg gaggtgtcgc
    32941 gcccctgtac gcgcacacct ccgatgccac cggagcccag ccatgaccaa cgaacagcta
    33001 tacctcgcaa tggccgttcc cattcttttc aacggcctca tgtttgctct gctgctcgcc
    33061 tacatgaacg ccaaattcaa gagcatcgaa gaacgcttca acgaacgctt cgacgccatc
    33121 aaccagcgct tcgagtccat caaccagcgc ttcgacgaca tgcgcgacct ctggcgcgct
    33181 gaactccacc gcgtcgaaga ggtccttgac gctcgcctca agcacctgga gggacgcata
    33241 tgaccatcga cgaacgcctt gaagccctca cccagaccgt cgagattatc gccggtatgc
    33301 agcaggaaac ggaacggcgg atgcaagaaa ccgagcgtcg catggcccag ctcgcggata
    33361 ccatgaaccg tctcgccaac gtcgtcatcc gccacgaaga gcgtctcgat gagctcgacg
    33421 gaggcaatcg tcaatgacgg atacctcgct ttagcgtttg ggaagcagcg aaacagaaaa
    33481 atccggtgta agctctctcc tgtgacatgc gcaagcgggc tgatacggct ggagcgatga
    33541 atccagacag ccacgaggtt ctcaaaggtg gccgtcagcc gaacggtgta tgaaagctgg
    33601 ctggaatggt ccaaaacggt tctgccgtcc actaattcag atctgaaaat cttgtaagat
    33661 cgaacggttc gcaatatact ggcggacgat ccggagagta atgcatttgt ccggtaagag
    33721 gagacctatt ccagattatg aagactgcat ttttctttct tttcatcacg gttgtgcttt
    33781 ggggacagga gcggttggtc actccggacg acccttattt caaataccag atctctttcg
    33841 ataatccggg cggaactttg acgattccac agctctcctt ccggctgtcg cccacagaga
    33901 cctataccac gcagcgcggg ttggaccata acatgcaacg cgcctgggct ctcacaaccg
    33961 gctcgaagaa catcgtggtc gcgattcttg acgatggatt tttctaccag catgaggacg
    34021 taaaagacaa catctggcgt aatccaggag agaccggagt ggacgctaac ggcctgcgca
    34081 aagagaccaa cggcgtcgac gacgatggca acggctatgt cgacgatgtc atgggatggg
    34141 acttcatctt ccgtgatccc gacccggacc cctatgtttt cgatggcatg gataccactc
    34201 gaattcaacc aaactggcac tccattgcag cgatgggcat catcggagcg aaggggaata
    34261 atgccatcgg agtagccggt atcaactggg atatttcgat gatgcttctg aagagaggag
    34321 cgcagggacg caagatgggc gacccggatc cggaagaagt aggccgggtt gtacgggcca
    34381 ttcgctatgc ggtagacaac ggtgcgcggg tgatcaactg gagtggctac acggaagacc
    34441 agcggcccgc ttcggtcgcg gcgctgaagg ccgccttcga atatgcggac cggcacggcg
    34501 tattgattgt gatcggtgcg ggaaatgaaa tgaagaacct ggaggatccg gcgaattgca
    34561 tttacctgcc ccagtgcttc gagatggaca acctcctcag tgtcgcgcaa ctggcatttg
    34621 agggagatct gttccgcttt aagggctctg agggcagcta cgtcagcggc tccaacttcg
    34681 ggccccggta cgttcacatc gcggctatc
//
LOCUS       DASTMK010000209        11315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_457576, whole genome shotgun sequence.
ACCESSION   DASTMK010000209 DASTMK010000000
VERSION     DASTMK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457576"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFQ79_09235"
     CDS             <1..204
                     /locus_tag="VFQ79_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139881.1"
                     /translation="YVRMTKSRGRAGTAGMYSFIAMLSILYVLNAAGPPPPGVRAIAF
                     AGIAGWLFPFWAAWFDRNRAIEL"
     gene            225..2174
                     /locus_tag="VFQ79_09240"
     CDS             225..2174
                     /locus_tag="VFQ79_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0139882.1"
                     /translation="MKRRDFMAGLAMGPYSIVVLADQIPPAAAPPPSPDEIRRQLDRV
                     APPKAGRVSSEPHMDLVDLETDFLVAGGGLAGVCAAITAARHGAKVILVQDRSRLGGN
                     SSSEVKMHVCGSHALPGWRETGLVEELRIEDAVHNPQRSFEIWDLMLYDKVISEPNIT
                     LLLESTLYSAKRKGDRIEEVMVRCDKSEIAYRIKAKYFCDATGDCRLGLEAGAEMRTG
                     REARSEFNESLAMEKADDETLGSSILFTSRLFPRPMPFKPPQWARKITREHLRMRRIT
                     EWDYGYWWIAWGGDKNTIRDNERIRFELLSIVLGVWDYIKNSGDFPDAAHWALDWVGM
                     MPGKRASRRMVGDYILTEHDLIRGDIEDAVCIGGWPMDDHPPEGFDRSDLRPNVAVQM
                     KEAYNIPLRSLYSKNISNLFMAGRNISCSHVAFTSTRVMATCGTAGQAVGAAAALCAA
                     NGILPRQIYENKELLAELQQGLLRDDQTIKSRTNKDPRDLALRAKVKASGEETGAAAV
                     RIIDGHLRDIPGKPQRQIHQWNARMSADGAWIELEWDQPQRIRHVQITFDTGLQRRLM
                     LSASASVTRGQIRAPQPETVRDYAILYRGAGADGLKEIVSITENYERLRRHYFEAVDA
                     SALRIHVKATNGDDLARIYEVRCYA"
     gene            complement(2471..3355)
                     /locus_tag="VFQ79_09245"
     CDS             complement(2471..3355)
                     /locus_tag="VFQ79_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEU0139883.1"
                     /translation="MPTAQAGIFALGTSSHVYLELDLVNENQCRDFAATVAAIREPRT
                     TTGGVNFVIGFRPELWQEMAPEQTPADIEGFNKNIEGVEDFVMPATQHDALVWMSGSA
                     YDVVFDMARMVIQDLAGKATLKEETSSWSYRYDRDLTGFVDGSENPTLLDAPVDALLP
                     DGAPGAAGSVLLLQKWKHRTAKWEALSVAEQELVMGRTKAESIELENKPADSHVARTD
                     QDKFGHIFRRNMPYGNVDNHGTVFVGFSAEQHRLTKMLESMAGLVDGTRDALTRYTQP
                     LTGAYYFVPSVENLRELR"
     gene            complement(3787..6405)
                     /locus_tag="VFQ79_09250"
     CDS             complement(3787..6405)
                     /locus_tag="VFQ79_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139884.1"
                     /translation="MRWRRKERERDLDREIEADLELEAAEQRDRGLSKEEACYAARRA
                     FGNMALLKEDTRESWGWTALERLLQDIRYALRTLRRSPGFTTIAVLSLALGIGANTAI
                     FTFVNAALLKPLPYPNADRIVALFQRPLKGDEPAAVSPRSFVPWHDRARSFEALAIAQ
                     SIPVNTQGADGAEQVSGLWTTPELFRVFGAQPMLGRLFTGEEGLNREAIRRGLAGTNS
                     VVVLSHGYWQRRFGADPNILEKTIPLERGAATVIGVLPPGFQLGSQGVDLYLPVPLDR
                     TRPEAIGSRAFHCFGLLRPGVTLDAARAEMEVLAVEVGRQDPIEKDWGVVVLSLRDYF
                     VRDNRLVLFVLLGVVVLVLLIACANLAGLLLTRGIGRRNELALRASLGVGRGRLLQQL
                     LIESMVLSAMGGALGLLLGSLASRALVVLAQDAVAFGQIADASLDARVFVFTVVLSLL
                     TAIVFGLAPASQVSRFDLQAALREQGRGASDSRGQQRLRSALVVGEIALAVVLLAGAG
                     LLLQTFASLLQVKLGFQPANVLTMRIFVTGDAARRSNLVESVLERIRTLPEVRAVGAI
                     QFLPLTGFTNNWVFHFAGRPEPVDTEQTQADGSTVSHGYLAAMGIPILRGRDFDRRDH
                     LNSPPVALVNESFVRKYCRNEEPIGQRIVGDWANSKPAEIVGVVGDIRQTALAAESRP
                     TIFLAQAQTPGYITYLVARTQSEPQRLAAAVRRAVRQVDPNQPVTAVQPMEQYVSTAL
                     AKPRLYGVLVGTFAALALLLAAIGVYGLMAYSVSRRTHEIGIRLALGAQRQEVLFSIL
                     GQGIRLMLIGLALGLACAVALSRFVANLLYGISAADPGTYVAVAILLGGISLAAAYVP
                     ALRASRVNPMTALRYE"
     gene            complement(6407..6763)
                     /locus_tag="VFQ79_09255"
     CDS             complement(6407..6763)
                     /locus_tag="VFQ79_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0139885.1"
                     /translation="MPKDSPNRIELLQGTLDMLILRTLLFGPAHGHQIAKHIQRTTDN
                     LLQVEHGSLYPALHRLERKGWIAGRWEPAGKDLKREFKYYRLTPAGKKQLVTEESKWK
                     QLTGVIARIMWPAGEN"
     gene            6908..7795
                     /locus_tag="VFQ79_09260"
     CDS             6908..7795
                     /locus_tag="VFQ79_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU0139886.1"
                     /translation="MNNIWLHRYAVLLVVCTLFLVIAGASVTSNEAGLSVPDWPLSYG
                     RLMPEMTGGVFYEHGHRMIATTVGFMTIILAVWLWRVEPRAWLRRLGWIALATVIFQG
                     ILGGITVLYLLPKVISIAHACLAELFFSLTAAIALFTSPAWKRGAEMVEDSGWPSLRS
                     MAVVTPVVVLAQVALGAAYRHGLMDIIPHIVGASVVTGLVLYLSVSLLMQYGFHRGLR
                     RAGLALTIVTFVQVFLGIAAYISRISTMDSVYPMAVMVFFTVLHVGVGALTMASSVLA
                     SIQIMRSVRPSAVEVPQTA"
     gene            7792..8652
                     /gene="cyoE"
                     /locus_tag="VFQ79_09265"
     CDS             7792..8652
                     /gene="cyoE"
                     /locus_tag="VFQ79_09265"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU0139887.1"
                     /translation="MRMMRDYLELTKPRVTWLILMSTGVGYFFGLRGHWDLLVLLHTV
                     LGTALIASGTFTLNQWYEREADSKMRRTQARPLPTGRVTPRKAFAFGIILSIAGSLEL
                     WLGANWLASVLGLFTLLSYIGLYTPLKQWTWHSTTIGAVPGAMPPLIGYAASAGTLTL
                     EAWVLFAILFFWQFPHFYSIAWLYREDYARAGIQMLPVVEPDGQSTARQILITSVLLI
                     PVSMAPSLLSMAGKLYLVGAFALGVAFLYSGLRVALERTRARARGVLIASVLYLPALY
                     SLMLLDRPAL"
     gene            8649..9560
                     /locus_tag="VFQ79_09270"
     CDS             8649..9560
                     /locus_tag="VFQ79_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139888.1"
                     /translation="MIVVVEDLSYSYDSRIAIQDLSFAVPKGQIFGILGPNGGGKSTL
                     FRILSTMMHPSSGRASIAGFDVRTHPAEVRRRTGVVFQSQSLDKKLTVAENLHSQGHL
                     FGLHGRALQVRIEDLVQRLGLHGRASDLVGTLSGGLRRRVEIAKVLLHEPSVLLMDEP
                     STGLDPGARRDLWNYIERLRREQDVTVLLTTHILEEADRCDRLLLLHQGRKVIEGPPA
                     ELKSRVGGDVVVLETASENGFEESLAARFGVQPHRVDGRLRVEIANGHRFIAEVVEAF
                     PGAIDSIALHRPTLEDVFLSETGALIE"
     gene            9553..10302
                     /locus_tag="VFQ79_09275"
     CDS             9553..10302
                     /locus_tag="VFQ79_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139889.1"
                     /translation="MNSGFWLPAFTLWRRELIRFWREKARVAGFVGSPLVFWLVIGSG
                     FGNLAFFFPGALTLTVMFSAVFSTMSLIEDRREGFLLSMLVSPAPRSAMVLGKVMGSA
                     TLAWIQGLIFLAFFPLAGFRPSAVAIVELVAVLFLISFTFTSLGFLLAWQMDSTQGFH
                     AVINLLLFPLWMVSGAIFSIESAHGWMQVLMRMNPLTYSLSLVRHFLERGNGEGLPPL
                     ATSLVVSLATGIALWMASTFVASQRTTRNFG"
     gene            10299..10853
                     /locus_tag="VFQ79_09280"
     CDS             10299..10853
                     /locus_tag="VFQ79_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0139890.1"
                     /translation="MRLAAVVAIVLCLAGCREAPRLPEYGTVPGFQLTSQTGDVFDSS
                     VLEGKIWVADFFFTTCVGPCPRMSAQMRQVGKRVEDLPDLRLVSFTVDPEHDTPEVLA
                     AYSKRFQASPKRWVFLTGPVETLNMLSFDSFKLGKVDNKNEHSTRFVLVDRRSRIRGY
                     YATWESGVIRQLVDDIRSLYRENS"
     gene            10853..11266
                     /locus_tag="VFQ79_09285"
     CDS             10853..11266
                     /locus_tag="VFQ79_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEU0139891.1"
                     /translation="MDFTYLSTVNAILNATAAALLIRGYLLIRRKRIDAHRKTMLAAF
                     CVSVLFLVSYLTYHYQVGSVRFQKTGMIRTVYFAILLSHTVLAAFVAPMAIITLVRGL
                     RGRWDKHRRIARWTFPLWVYVSVTGVLVYLMLYHF"
BASE COUNT         2139 a         3360 c         3428 g         2388 t
ORIGIN      
        1 tatgtacgca tgacgaagag ccgtggccgt gccggtaccg ccggcatgta cagcttcatc
       61 gctatgctct cgatccttta tgtgctgaat gcggccggac ctccgccgcc gggcgtacgc
      121 gccatcgctt tcgccgggat cgccggttgg ctcttccctt tttgggcagc atggtttgat
      181 cgcaaccggg cgatagagct atgaatcttt tagagaggat tagaatgaag agacgcgatt
      241 tcatggcggg cctcgcaatg gggccgtact cgatcgttgt gttggcggat cagattccac
      301 cggccgcggc gccgccccca tcaccggatg aaatccgccg gcaactggat cgcgtcgctc
      361 cgccgaaggc cgggcgcgtg tccagcgaac cccacatgga cctcgtcgat ctggagaccg
      421 atttcctggt cgccggcgga ggattggcgg gggtatgcgc cgccatcacg gcggcacgtc
      481 atggcgccaa ggtgattcta gtgcaggacc gttcgcggct cggtggcaat tcctcgagtg
      541 aagtgaagat gcacgtgtgt ggctcgcacg cgctgcccgg ctggcgcgaa acgggtctgg
      601 tggaggagtt gcgcatcgag gatgccgtcc acaatccgca gcgctccttc gagatctggg
      661 acctcatgct ttacgacaag gtgatttcgg aaccgaacat caccctgctg ctcgaaagca
      721 cgctttacag cgcgaaacga aaaggcgatc ggattgaaga ggtcatggtt cgctgcgaca
      781 agagcgaaat cgcctatcga atcaaggcga agtatttctg cgacgcgacc ggagactgcc
      841 ggctggggct cgaagcgggc gccgaaatgc gcactggcag ggaggcgcga agcgaattca
      901 acgaatcgct ggccatggaa aaagcggacg acgagacgct tggcagcagc atccttttta
      961 catcgcgcct gttcccgcgc cccatgccgt tcaagccgcc ccagtgggcg cgaaagatca
     1021 cgcgggagca tctgcggatg cggcgaatca cggaatggga ttacggctat tggtggatcg
     1081 cctggggcgg tgacaagaat acgattcgcg ataacgagcg catccgcttc gagctactgt
     1141 cgattgtgct cggtgtctgg gactacatca agaacagcgg cgacttcccc gatgccgcgc
     1201 actgggcgct ggattgggtg ggcatgatgc ccggaaaacg cgccagccgc cgcatggttg
     1261 gcgattatat tctcaccgag cacgatctca ttcgcgggga catcgaagat gccgtctgta
     1321 tcggcggctg gccgatggac gatcatcccc ccgaggggtt tgatcgctcg gacttgcggc
     1381 ccaacgtcgc cgttcaaatg aaggaagcct acaatatccc gctgcgttcg ctgtacagca
     1441 aaaacatctc gaatctgttt atggcaggcc gcaacatcag ttgctcccac gtggcgttca
     1501 cctccacgcg ggtgatggct acttgcggca cggcggggca ggctgtggga gccgccgcgg
     1561 ctctctgtgc ggccaatggc atccttccga ggcagattta cgagaacaag gaattgcttg
     1621 cggaactgca gcaggggctc ctgcgcgacg atcagacaat taagagccgg accaacaagg
     1681 acccgcgcga tctggcgcta cgggcaaagg tgaaggcttc gggcgaagag accggggcgg
     1741 cggctgttcg tatcatcgac ggccaccttc gcgatatccc tgggaagccg caaagacaga
     1801 ttcaccagtg gaacgcccga atgagcgcgg acggggcctg gatcgaactg gaatgggacc
     1861 agccgcagcg catccggcat gtgcagatca cgttcgatac gggtttgcag cgaaggctga
     1921 tgctgagcgc ctccgcgtcg gtgacccgag gccagatccg tgctccgcag ccggaaaccg
     1981 tccgcgacta tgccattctc tatcgcggcg ccggcgcgga cggcctgaaa gagatcgtgt
     2041 cgatcacgga aaactacgaa cggcttcggc ggcattactt cgaagcggtc gatgcctcgg
     2101 ctctgcgcat tcacgtgaaa gccaccaacg gcgatgatct cgcccgcatc tacgaggtgc
     2161 gctgctacgc atgagaagca gccggtttag gcccatactg ttcacttaaa tgctaggatg
     2221 gccggaccgc cgcccttgac attactttcg ccataggtga acaaaaccgg tcaataatgt
     2281 ggagtttgta aatgctgcgt gtcaggggag atctatcaac caagtcatgt atcagcgttg
     2341 ggtgtgatac gtcaaggtgg agtacttccg ggtagcgcgt cgctcgtcaa caatcgccaa
     2401 accataagcc tgctttctgg tactaatccc aagccggcgt ttgtaatgat tagactgagc
     2461 gcttcgcaat ctaccgcagc tctcgcaggt tttcaaccga aggcacaaag taatatgctc
     2521 ctgttagcgg ctgcgtataa cgcgtcagcg catcacgcgt gccgtccacc aggcccgcca
     2581 tgctctcgag catcttcgtg agccggtgct gttccgcact aaaacccaca aagacggtac
     2641 cgtggttgtc gacattccca tacggcatgt tgcgccgaaa gatgtgtccg aacttgtcct
     2701 ggtccgttcg cgccacgtgc gagtccgcag gcttgttctc caactcgatg ctttcagcct
     2761 ttgtccgacc cattaccagc tcctgctccg ccaccgacaa tgcttcccac ttcgcagtcc
     2821 tgtgtttcca tttctgcagg agcagcacag aacccgcagc accgggtgca ccgtcaggca
     2881 ggagcgcatc gacgggtgcg tccagaagcg ttgggttctc cgacccatcc acaaaacccg
     2941 tcaggtcgcg gtcgtaccgg taggaccaac ttgaggtctc ttctttcagc gtggctttcc
     3001 cggcgagatc ctgaatcacc atccgagcca tgtcgaacac cacgtcatac gcgctgcccg
     3061 acatccacac caacgcatca tgctgcgtcg ccggcataac aaaatcttcg acaccctcga
     3121 tgttcttgtt gaatccttct atatcggctg gcgtttgctc tggcgccatt tcctgccaca
     3181 actcgggacg aaagccgatc acgaagttga ccccgcccgt ggtcgtccgt ggctcgcgga
     3241 tcgcagcgac agtagccgcg aagtctctac actgattctc gtttacgaga tccaactcca
     3301 ggtagacatg cgaggacgtt ccgagagcaa agataccggc ttgtgccgta ggcataccag
     3361 aagtatagcg agtacgattg ttaccggcac cgatggagcg acgtaaagat gaaaacctgt
     3421 ggggctttca tcaggtaatt acaccggaga tgactcgctc gataggtctg gatttttaag
     3481 ccccaacaaa ggccttgcat ctggcgactt ctggattgtc ggccatccag aagcaatcca
     3541 catgagcgtg tagtcgccta tgcgtggaag tgccgacgaa cttccaaggt ttagtaactg
     3601 attcgcagcg ggcggcccca acagccttgt gtcttccgca ttagcggtga aagaggaacc
     3661 gcttgggcgg tggcctcaac agcattgggc tttaggccca agtcgtgctg cctggtctct
     3721 gatacggcac cggcaacggc tttcatgaat aacatctggc tccatcgaga tggctgtact
     3781 cgccggtcac tcgtatctca aggctgtcat cgggttgacg cgggaagccc gaagcgcggg
     3841 gacgtaggcc gccgcgagcg aaataccgcc gagtaagatg gccacggcaa cgtaggtgcc
     3901 tggatcggct gcgctaatgc catagaggag attggcgaca aaccggctca acgcgacagc
     3961 acacgcaaga ccaagagcca gcccgatcag catcagccgg atgccttgac cgaggatcga
     4021 gaacaggacc tcctgccgct gggcacccaa agcgagccgg attccgatct cgtgggtgcg
     4081 ccggctgacc gagtacgcca tcagtccgta cacgccaatt gccgccagaa gcagtgcaag
     4141 tgcggcaaac gtgccgacca gaactccata cagcctcggt ttcgccagcg ccgtggacac
     4201 gtactgctcc atgggctgga ccgcggtcac cggctggttg ggatccacct gccggactgc
     4261 gcggcgaacc gccgcggcca gcctttgagg ctccgattgt gtgcgcgcca cgaggtacgt
     4321 aatgtagccg ggggtttgcg cctgtgccag aaagattgtg ggccgggact ccgccgccaa
     4381 tgcggtttgg cgaatatcgc ccaccacgcc cacaatttcc gccggttttg agttcgccca
     4441 gtcgccaacg atgcgttgac cgatcggctc ctcgttccgg cagtactttc tgacgaacga
     4501 ctcattcacc aatgccacgg gagggctgtt cagatggtcg cgcctgtcga aatcgcggcc
     4561 ccggagaatc gggataccca tcgcggccag gtatccgtga ctcaccgtgg acccatcggc
     4621 ctgcgtttgt tctgtgtcca cgggctccgg ccgcccggca aagtggaata cccagttatt
     4681 ggtgaagcct gtcagcggaa ggaactggat cgcacccacg gcacggactt ccggtaatgt
     4741 ccttatccgc tcgagaacgc tttcgaccag attcgatcgc ctggctgcat cgcccgttac
     4801 aaaaatacgc atggtcagaa cgtttgccgg ctggaagccg agcttcactt gaagaaggct
     4861 ggcgaacgtc tgaaggagaa gaccggcgcc ggccagcagc acgacagcaa gcgcgatctc
     4921 tcccacgacg agagcggatc gaaggcgctg ctgcccgcgg ctatcgctgg cgccgcggcc
     4981 ctgttccctg agcgctgcct gcaaatcgaa tcgcgatacc tgcgacgccg gcgccagccc
     5041 gaacacaatg gccgtcaaca gagatagaac cactgtgaac acaaacacac gcgcatcgag
     5101 agatgcatcg gcgatctgtc caaaggcgac cgcgtcttgt gcgagaacaa ccaaggcgcg
     5161 gctggcaagc gagccaagga gcaggccgag agcgccgccc atcgcggaaa gcaccatgct
     5221 ctcgatcagc aattgctgca acagccggcc ccggcccacg cccagcgaag cccgcagcgc
     5281 caactcattg cggcggccga tgccgcgtgt caggagcagg ccggccaaat tggcacaggc
     5341 gatcagcagg accagaacaa ccaccccgag cagaacaaaa agcaccaggc ggttgtcccg
     5401 cacaaaatag tcccgcagac tgagcacgac cacgccccag tctttctcga tggggtcctg
     5461 acgtccgacc tccacggcaa gaacctccat ctctgcgcgc gctgcatcga gagtgactcc
     5521 cggacggagc aatccgaagc agtggaaagc ccgcgagccg atcgcctccg gtctcgtccg
     5581 gtccaatggc acaggaaggt agagatcaac tccttggctc cccagttgga agccgggagg
     5641 cagtactccg atcacagtgg cggcgccgcg ctccagtggg atggtttttt ccaaaatgtt
     5701 ggggtccgca ccgaagcgcc tctgccaata accgtggctc agcacgacca cggagttcgt
     5761 accggccaac ccacgccgga tggcctcgcg gttgagccct tcctcgccgg tgaacaggcg
     5821 gcccaacatg ggctgggcgc cgaatacgcg gaacaattcc ggcgtcgtcc agaggccgga
     5881 tacctgctcg gcgccatcgg cgccctgcgt gttgaccgga atcgactgtg cgatggcgag
     5941 cgcttcaaaa gaccgggcgc ggtcatgcca cggaacgaag ctgcgagggg agacagccgc
     6001 cggctcgtct cccttcagcg ggcgttgaaa cagcgccacg attcgatcgg cgttcggata
     6061 agggagcggc ttcagaagcg ccgcgttgac gaaagtgaag atggccgtat tggcaccgat
     6121 gccgagcgcg agcgaaagca ctgcgatcgt ggtgaatccc ggactcctgc gcagggtccg
     6181 gagagcgtag cgtatatcct ggagcagtct ttcgagcgcc gtccagcccc aggattcgcg
     6241 cgtgtcttct ttcaacagcg ccatgtttcc gaaagcgcgc cgcgccgcat agcaggcttc
     6301 ctccttggac agcccccggt cccgctgttc ggcggcttcc aactcgagat cggcttctat
     6361 ctcgcgatcg aggtcccgct cccgttcttt ccgccgccag cgcatctcag ttttccccgg
     6421 ccggccacat gatccgcgca atgacgccgg ttaactgctt ccatttcgac tcctctgtca
     6481 cgagctgctt cttaccggcc ggagtgagcc ggtaatactt gaactcgcgc ttcagatctt
     6541 tgcccgcggg ttcccatctc cccgcgatcc agcccttgcg ttccaggcgg tgcagcgccg
     6601 gatagagcga tccatgctcg acctgcagca ggttgtccgt agttctctgg atgtgtttcg
     6661 cgatttgatg cccgtgagcg gggccgaaca gcaaagtacg cagaatcagc atgtccagcg
     6721 ttccttgcag caattcgatc cggttggggg agtctttcgg catgttcttc gccacactgg
     6781 gtagacggac taccgagaaa aaagttagcg ggccctactc ccgccacttt cgagtggcag
     6841 gtgacataga atgtaaacat acaggtcgtg cggcctggtc tctggtacgg cgtctgcaac
     6901 ggctctcatg aataacatct ggctccatcg atacgctgta ttgctcgttg tctgcacctt
     6961 atttctggtt attgccggcg cttccgttac cagcaatgaa gcagggttgt ccgtgcccga
     7021 ctggccgctt tcctacgggc ggctcatgcc cgaaatgacg ggcggcgttt tttacgagca
     7081 cgggcatcgc atgattgcga cgactgtcgg tttcatgacc atcatccttg ccgtgtggct
     7141 ctggcgggtg gaaccacgcg cctggctgcg ccgcctgggg tggatcgcat tagcaacggt
     7201 gatttttcag ggcatcctgg gcggcatcac cgtgctctac ctgctgccga aggttatcag
     7261 cattgcacat gcctgcttag ccgaactctt cttttcgctg acagccgcca ttgccctgtt
     7321 cacgtcgccc gcctggaaac gcggggcgga aatggtggag gattccggat ggccgtcgct
     7381 gcgctcgatg gccgtcgtga ctccggtcgt tgtgctggct caggttgcgc tcggcgcggc
     7441 ttatcgccac gggctgatgg acatcattcc gcacatcgta ggcgccagcg tcgttaccgg
     7501 cctggtactg tatctctccg tcagcttgct catgcagtac ggattccacc ggggactccg
     7561 gcgggcgggc cttgcgttga cgattgtgac gtttgttcag gtatttctgg gaatcgcagc
     7621 ctacatcagc cgcatctcca ctatggatag tgtttacccg atggcggtga tggtcttttt
     7681 cacggtgctg catgtaggag tcggcgcgct gacgatggcg tcgagtgttt tggcttcgat
     7741 tcagattatg cggagcgtgc ggccatccgc tgtcgaggtg ccgcagaccg catgagaatg
     7801 atgagagact acctcgaact gaccaagccc cgcgtcacct ggctgatcct gatgagcacg
     7861 ggtgtcggat acttttttgg gcttcggggc cactgggatc tgttggtgtt gctccatacg
     7921 gtattaggaa ccgcattgat cgcttccggt acatttacct tgaaccaatg gtacgaaagg
     7981 gaggcggatt cgaagatgcg ccgcacgcag gcgcgccccc tgccgaccgg acgtgttacg
     8041 ccccggaaag cgttcgcatt cggcatcatt ttgtctattg ccggctcgct ggagttgtgg
     8101 ctgggtgcaa actggctggc atccgtgctg gggctgttca cgctgctgag ctacatcggt
     8161 ctatacacgc cgctgaagca gtggacgtgg cattccacga ccatcggcgc cgtgcccggc
     8221 gccatgcctc ctctcatcgg atacgcggcg tccgccggca cgctcaccct ggaagcgtgg
     8281 gttctgttcg caattctgtt tttctggcag tttccccact tctattcgat tgcctggctg
     8341 tatcgggagg attacgcgcg cgccggcata caaatgctgc cggtagtgga gccggacgga
     8401 caatcgacgg cgcgccagat cctgatcacg tccgtgctgt tgattcctgt cagcatggcg
     8461 ccgtcgctgt tgtcgatggc ggggaagctt tacctggtcg gcgccttcgc gcttggtgtg
     8521 gcgttcctct attcaggttt gcgcgtcgcg ctggagcgca cccgggcccg cgcgcgcggc
     8581 gttctcatcg cttccgtgct gtatctgccg gcgctgtata gtctcatgct tctcgatagg
     8641 ccggccttgt gattgtagtt gtcgaggacc tttcttacag ctacgattcg cgcatcgcga
     8701 ttcaggatct ttcctttgca gtgcccaagg ggcagatttt cggcatatta ggcccgaacg
     8761 gaggcggcaa gtccactctc ttccggattc tctcgaccat gatgcacccc tcgtcggggc
     8821 gcgcctccat agccgggttc gatgtacgga cacatccggc cgaggtgcgc cggcgaaccg
     8881 gcgtggtctt tcaatcgcag agcctcgaca agaaactgac ggtggcggag aatctgcaca
     8941 gccagggaca tctgttcggg cttcatgggc gcgcgctgca agtccggatc gaggatctgg
     9001 ttcaacgcct cggcttgcac ggccgcgcct ccgacctggt tggaacgctt tccggcgggt
     9061 tgcggcggcg cgtggagatc gccaaggtgt tgctgcatga accatcggtc ctgctgatgg
     9121 acgagccttc caccggtctc gatcccggag cacgccgcga cttgtggaac tacatcgagc
     9181 gcctgcggcg ggagcaggat gtcactgttt tgctgaccac tcatatcctg gaggaagcgg
     9241 accgctgcga ccgcctcctt ctgttgcacc agggcaggaa agtgatcgaa ggacctcccg
     9301 ccgagttgaa atcgcgtgtg ggaggcgatg tcgtcgttct ggaaacagcc tccgaaaacg
     9361 ggttcgagga gtcgctcgcg gcgcgcttcg gcgtgcagcc gcatcgggtg gacggcaggc
     9421 tcagggtcga gattgccaat gggcaccggt tcatcgcgga agtggtggag gcatttccgg
     9481 gcgccattga ctctatcgcc ctgcaccggc ccacgctgga agatgtgttt cttagtgaga
     9541 cgggggcgtt gattgaatag cggattctgg ctgccggcat tcactctatg gcgccgggaa
     9601 ctgatacggt tctggcgcga gaaggcgcgc gtggccggat ttgtgggctc ccccctcgta
     9661 ttctggctgg ttatcggatc cggtttcggc aacctggcgt ttttctttcc gggcgcgctc
     9721 accttgacgg tgatgttttc ggccgttttc tcgacgatgt cgctcatcga agaccggcgc
     9781 gaagggttcc tgctttccat gctggtttca ccagcgccgc gcagcgccat ggtattggga
     9841 aaggtgatgg gttccgcgac gctggcgtgg atacaagggc tgattttcct ggcatttttc
     9901 ccgctggcgg gcttccgtcc ctctgccgtg gctatcgtgg agcttgtcgc ggtgctgttt
     9961 ctgatttcgt tcacgttcac gtcgctcggg ttcctgctgg cgtggcagat ggattctacg
    10021 caggggtttc acgccgtgat taacctgctg ctttttccgt tgtggatggt ttcgggagcg
    10081 atcttctcca tcgaaagcgc gcacggttgg atgcaggtcc tgatgcgcat gaatccgttg
    10141 acctattccc tctcgcttgt acggcatttt ctggagcgcg gcaatggcga aggtttgccg
    10201 ccgctcgcca ccagtctcgt tgtgtcgctc gccaccggca tagcgctctg gatggcatcg
    10261 actttcgtgg cctcccagag gacgacgagg aacttcggat gagactggcg gccgttgtgg
    10321 ccatcgtgct ttgtcttgcc gggtgccgtg aagcgccgcg gcttcccgag tatggaacgg
    10381 ttcccggatt tcagttgacc tcccagaccg gggatgtttt tgacagcagc gtactggaag
    10441 gcaaaatctg ggtggcggat ttctttttca cgacgtgtgt agggccatgt ccccgtatga
    10501 gcgcgcaaat gcggcaggtc ggcaagcgtg tggaagatct gcccgacctg cgcctggttt
    10561 ccttcacggt ggacccggaa cacgatactc cggaagtgct tgccgcttat tcaaaacgct
    10621 tccaggcgag cccgaagagg tgggtttttc taacgggacc cgtcgagact ctgaatatgc
    10681 tgagtttcga cagcttcaag ctgggcaagg tggataacaa gaacgaacac agcacgcgtt
    10741 ttgtgctggt cgaccggcgt tcgcgcatcc ggggttatta cgccacctgg gaatccggcg
    10801 tgatccggca actggtggac gatatccggt cgctataccg ggagaattcc tgatggattt
    10861 cacttatctc tccacggtga atgcaatcct caacgcgacg gcagccgccc tgctgatacg
    10921 cggctatctg ctgatccggc gcaagaggat cgatgcgcac cgcaagacca tgctggcggc
    10981 gttctgcgtc tccgtgcttt ttctggtcag ctatcttacg tatcactacc aggtaggctc
    11041 cgtacggttt caaaaaacgg ggatgattcg cacggtgtat ttcgcgattt tgttgtccca
    11101 taccgtgctg gcggcgttcg tcgcgccgat ggcgattatc acgctcgtgc gcggattgcg
    11161 gggaagatgg gacaaacatc gccggattgc acgctggacg tttccgctct gggtgtacgt
    11221 cagcgtgacc ggcgtgctcg tgtatctgat gctgtaccat ttctgacgtt cgtacggacg
    11281 tgggacggtt ctggaaaccg tcccacatgc cgtca
//
LOCUS       DASTMK010000210        17950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_234036, whole genome shotgun sequence.
ACCESSION   DASTMK010000210 DASTMK010000000
VERSION     DASTMK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17950
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234036"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VFQ79_09290"
     CDS             complement(<1..1457)
                     /locus_tag="VFQ79_09290"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HEU0139892.1"
                     /translation="MARQQISVESVLDSGDPSIYDIRSKTSGPEGALPLTEEMLLTRP
                     SGDLFGWTMNAGMGWDPAKLGGKEVLILSTHGGIRAPDGTPIALGYHSGHWEVGLLME
                     AAARELKDLDAVPFAAYCTDPCDGRTQGTTGMFDSLPYRNDAATIFRRQIRSLPTRSA
                     VIGVATCDKGLPAMMMALAAIHELPCILVPGGVTLLPEEGEDAGLVQTIGARYAHGQI
                     TLEHAAEMGCRACASPGGGCQFLGTAATSQVVGEALGMSLTHSALAPSGHPLWLDMAR
                     RSARAVLALESRGLTMSKLLTSASVRNAMVTHAAFGGSTNLLMHLPAIAYSGALRRPS
                     VEDWAAVNRRVPRLVDVLPNGPRNHPTIQVFLAGGVPEVMLHLRRAGLLELDALTASG
                     ETLGTMLDWWEHSERRARLRTVLKERDGVDPDDVIMDPATARRRGLTSTVCFVGGNLA
                     PEGAVIKATAIDPDVVDEDGVYRKTGPARVFTTER"
     gene            1496..2833
                     /locus_tag="VFQ79_09295"
     CDS             1496..2833
                     /locus_tag="VFQ79_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139893.1"
                     /translation="MSSTRRCFLGAMTAASCQRVVGANDRVRVGFIGYGLIGAQHVFD
                     FKNQKDCDPAAMCDVYQPRLEQGVAACGGAAKGYRDFRKLLDDTSIDAVVISTPDHWH
                     APMAILACAAGKDVYVEKPLTLFVREGRWLVTAARRYNRIVQVGTQQRSGLHYQEALG
                     LLRSGVIGRIHSVRMASFRNVMPGFGRPPDSTPPSDLNYDLWLGPAPRRTYNPHRALY
                     HFRWFWDYSGGQMTNLGAHQIDILQWVLELNGPTAVSSAGGRFVLEDDGETPDTQDAV
                     FEYPGLTAVWSHREASAGRGASDLEFCGTKGSMTVLRSGFEVFADVKRNPEDAIPVFQ
                     GTPAGGPKRSASPRAYWTTPVKKPGSSAQQLDLHVRNFLDCVKSRQKPIADVEEGHRT
                     ATTCHLANISLRIGRKIRWDQASEQIPGDSEASAMLERPYREPWAGIIGSLRL"
     gene            2830..3816
                     /locus_tag="VFQ79_09300"
     CDS             2830..3816
                     /locus_tag="VFQ79_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0139894.1"
                     /translation="MTRRELLLAGSSAAFLRGQNPRVRIGIATTCYRAARRPKDTIGF
                     LEFVHSAGGGGIQASLSSLDPVYLTRLRRRAEELDMYVEVMVDLPHTDTTPFERTVQA
                     AKYAGALCMRAACLSGRRYETFDSLGEWKEFVARSHSSLRQAARIVEKHRIPVAVENH
                     KDWTADELESLMKQYGGDYLGVCLDTGNNIALLDDPMEVVERLAPNAFSTHIKDMAVD
                     TYPDGFLLSEVPVGEGILDIKRIVSSVQRARPKTRLTLEMITRNPLPIPCLTDKYWAT
                     FPASRRPYLERTMRLVKERRSDLPRLDGLDDDAQHKLEDTNVTRCLRYFGNG"
     gene            3874..4512
                     /locus_tag="VFQ79_09305"
     CDS             3874..4512
                     /locus_tag="VFQ79_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0139895.1"
                     /translation="MKSMALNLQEWRRQNSLTQVEAAALLGVSQPYLSLLEKGVRPLT
                     KTVRARMKSVNRARPHISLDDRFREQLSALGYAPFAHVSPARLKPAPDVFLLSVLMQP
                     DVDARVVEALSWVVQTYAAKMNFPWLVQQAKLENLQNQMGFLFRVSDSKSPELAVALD
                     ALEKARLLKEATLCWDSMPSATRKWMRQHRSALARHWNLVTTLQQEQSMHAA"
     gene            4502..4696
                     /locus_tag="VFQ79_09310"
     CDS             4502..4696
                     /locus_tag="VFQ79_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139896.1"
                     /translation="MRRSCPEDKPAEPWRSFLTELDRFLDDSVDFHCIGGFVISQFYG
                     LGRETADLDILRVIPKYIVE"
     gene            5174..5668
                     /gene="purE"
                     /locus_tag="VFQ79_09315"
     CDS             5174..5668
                     /gene="purE"
                     /locus_tag="VFQ79_09315"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU0139897.1"
                     /translation="MDHQPKPLVAVIMGSKSDWDVMRQTAETLTRFDVPHECRVLSAH
                     RTPAAAAEYVSGAESRGVEVVIAAAGGAAHLAGVAAAHTILPVLGVPMESASLKGLDS
                     LLSTVQMPGGIPVGTLAIGKAGATNAALLAVAILGVKRPELRDKLRRFREEQANKILS
                     ETLA"
     gene            5719..6873
                     /gene="larC"
                     /locus_tag="VFQ79_09320"
     CDS             5719..6873
                     /gene="larC"
                     /locus_tag="VFQ79_09320"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEU0139898.1"
                     /translation="MKICYLDAFSGISGDMTVAALIDAGADAKTLIECLNSLETGASF
                     RVEKTKRRGIAASKFHVDVEDTRAHRHLPQILRMIERAKLPDGAREQATAIFEKLGEA
                     EASVHWVPIEKVHFHEVGAVDSICDIVGAAVGFRLLDVSQVYCSPLNVGSGTVHTEHG
                     VLPVPAPATAKLLSGKPVYSRGPEVELTTPTGAAIATTLAAGFGPMPPMRISSVGYGA
                     GDLEFAEHANVLRILVGEASQATEATTVAVIEANIDDASPQMLGYAMERLLENGALDV
                     TMTPLFMKKNRPGTLLSVIARPEDRERLCGLVFAETPTLGLRVYAAERRVEERRIVEV
                     DTAHGKIRMKVSGAGFAAPEYEDCRRLASEKGIPLRVVLADATNAYLNKR"
     gene            6870..8813
                     /gene="metG"
                     /locus_tag="VFQ79_09325"
     CDS             6870..8813
                     /gene="metG"
                     /locus_tag="VFQ79_09325"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU0139899.1"
                     /translation="MKYYLTTPIYYVNAAPHIGHAYTTIVADLVRRFKRMQGYEAVLT
                     TGSDEHGVNVERAAQRAGKSAKEFCDVISEEFRVQWERLGLSVDRFQRTTSPQHAKVV
                     QDLFERCRRNGYVYKGSYTGQYCPFDNLYVNDAKPGDPCPDCGRPTETVTEENFFFKL
                     SAFADRLLDLYETQPDFIQPATRRNEVIAFVKQGLQDLSITRTNIKWGIPVEGEAPHV
                     FYVWFDALSTYMSAVEGEGLWPADLHLIGKEIVRFHAIYWPAFLWAAGLELPKRIYAH
                     GWLLFESDKMSKSRGNIVRAIPISQVIGVDALRYFLLREIVFGQDGSFSYDALVGRYN
                     SDLANGLGNLASRTLSMIHQYRGGAVPSGTPAASSAVATQAADTIAAVIAAFENFEFS
                     KGLETLWSLLSVVDRFIVEKAPWVLAKKEDEASQAVLNETLYTAAEVLRIATVLLAPV
                     LPESAARIWRQLGMTQPLEEVRFAGLSWGQLAAGQMIGEATAVFPRIDAKPAIDKMRE
                     LEKQETARQAALFGKTAPETPGAAAAPQGDGMITIDDFSKIDLRVGEVRSAERVKGAD
                     KLLHMMIDIGEERPRSIVAGIALAYTPEQMVGRKVVIVANLQPRKLRGIESQGMIVAA
                     SLADGAPVLAGFLEDVPIGARLK"
     gene            8810..9637
                     /locus_tag="VFQ79_09330"
     CDS             8810..9637
                     /locus_tag="VFQ79_09330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU0139900.1"
                     /translation="MSLTDTHCHLDDEKFDSDRDAAIERAFEAGVTQIVAVGTGDGPP
                     DLEAGIRLADAHQNVYATVGVHPHDTSKADDGSLRRLEELLKHEKVVAVGEVGLDYHY
                     DFSPRDRQRAVFVEQMRIARDARKPIVIHTREAWDDTIALLAEHWTPSGLGGIMHCFT
                     GGAAQAAQCLQLGFHLSFAGILTFPKALEIREAAAMCPMDRLLVETDAPYLAPIPHRG
                     KRNEPAFVVHTAQALAGLRGLTLEEVARATTHNFGRLCLPENRDSGYTETIPWDSAS"
     gene            9619..10620
                     /locus_tag="VFQ79_09335"
     CDS             9619..10620
                     /locus_tag="VFQ79_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU0139901.1"
                     /translation="MGFSKLGQSFTAREILELVQDDLRKVEREIRLESVASVDAITTI
                     GKYLQDGGGKRLRPTLLLLACRLFSEPPESAIRLAAVIEIIHTATLVHDDVIDIAKTR
                     RGRPSPNVIWGNHTSVLAGDWLYMQAFQMALRERSFHVLDLLIGLTQMMVEGELLQLD
                     RIRKIDISEADYMELVDRKTASLFSVAARLGAITAGADDVSEARLGEYGWSLGIAFQL
                     VDDVLDFTAHESVLGKPVGNDLQEGKVTLPLIYALERTTGSERELVETVLADANYHRV
                     PFSTILELIERHDGISRAKERAQMFTDKARSIAMEFPESPYQRALVALTDLVTERDY"
     gene            10627..11976
                     /locus_tag="VFQ79_09340"
     CDS             10627..11976
                     /locus_tag="VFQ79_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEU0139902.1"
                     /translation="MKTRREWLTGAGSLLGAAAGGAFASQKQRGRRPRILLRSSWQSV
                     NIGDIGHTPGALSLLNKHFPEAGITLWPGRLDQASRDLLTHNYPDLKIVEGTVGADGR
                     PTPELARAWAETGLCLSGSGSGFPASAHAVAFHKATGKPVGVFGVSTDPISGIGEGRE
                     PEGGTLKELRAKALRLPPGHLPADLRYIIDRAAFFFCRDTISRDYLRAQKVKTPILEF
                     GPDAQLGMHIRDDAKGLAYLKASGLEEGRFICVIPRLRYTPYYRIRGTPRTPADDIRD
                     AINSRTTEADHAKLREMIISYVKNTGNRVLACPEMTYQIELAKEVLVDPLPEAIRKSV
                     VWRDSFWLPDEAASVYARAQAVISVECHSPLIALHNGTPAMYVRQPTDTCKGQMYRDF
                     GAGRWMFEIEETSGGELWSRVAEIVKDPGKARVRVRSILSGVEDRQKRMVAAVRAAC"
     gene            complement(11983..13557)
                     /locus_tag="VFQ79_09345"
     CDS             complement(11983..13557)
                     /locus_tag="VFQ79_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0139903.1"
                     /translation="MAGSDDVNEIQGEASGSPPGRDLLQRLGIEAIHSGACWGDWIPE
                     PEGGELISSSPHDGKEIARIRMAAEEEYDEVVWHAADAFHDWRLLPAPRRGDIIRDIA
                     GELRRVKDDLGALVSLEMGKILPEGKGEVQEMIDIADFAAGLSRQLYGLSMHSERPGH
                     RMYEQWHPLGPVGVITSFNFPVAVWAWNAMIAAVCGDCVVWKPSSETPLCAIAVQNIC
                     NRVLERHGWRGVFNLVIGKSSVVGERMSKDRRIPLISATGSTSMGYRVQEAVGNRLGR
                     TLLELGGNNGVIVMDDADLDLALRAVLFGAVGTAGQRCTTIRRLFLQKGIAPKLTGML
                     LNVYRQIRIGDPLRGDTLMGPLINRAAVKDMMEGLSQAREQGGEVIFGGKALDRAGGC
                     YVKPTLVKARPEMPILKEELFAPVLYLIEFDTLDEAIEWHNNVPQGLSSSIFTTNLIS
                     AETFLSARGSDCGIANVNIGTSGAEIGGAFGGEKETGGGREAGSDAWKTYMRRQTVTI
                     NWSSDLPLAQGIEFTL"
     gene            complement(13638..16115)
                     /locus_tag="VFQ79_09350"
     CDS             complement(13638..16115)
                     /locus_tag="VFQ79_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU0139904.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGSPYIETEHLLLGLLREDKAL
                     ANRFLRSVAAIESIRKQIEAHTTIREKVSTSVDLPLSHECKRVLAYGAEEAERLNHKH
                     IGTEHLLLGLLREEKCFAAEILHERGLRLSQVREEIARSSSEKMASSRPKESSLLSEF
                     SRDLTQAAMDGMLDPLIGRDYELERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQR
                     IADGDVPSFLSDKRILALDLSLIVAGTKYRGQFEERLKTIMKELMENQNAIIFIDELH
                     TLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPGEYRKSIEKDRSLERRFQAVKVP
                     PPTEADAVRILFGIKERYEKFHAVAYTDDSIESAVYASSRFIPDRYLPDKAIDLIDEA
                     GARVKLRQTTLPGEVADVQKRIKFIVHRMENAIANHEFEKARFYSDEERKERENLRLL
                     REKYNLDDTSTGVVGKDDIEDVVARWTGVPMTAIREEEVAKLLRIEEELHKRVISQEK
                     ALSALSRAIRRSRAGLKSPKRPAGSFLFLGPTGVGKTEVARALAEFLFGSEKSLIRFD
                     MSEFMEKHSVSKLIGSPPGYVGYEEGGQLTERVKRSPYSVILLDEIEKAHPDVYNILL
                     QVFEDGQLTDGLGNTVDFKNTIIIMTSNLGARFLDKKSPMGFSAPQEQGIPSKIEDQV
                     LSEVKRAFNPEFLNRLDEVILFTSLTDDDLIKIIDLMVLQINSNLVDKQITIRLAPDA
                     ARYILEKTCADRSYGARPLRRALQKYIEDPLSEALIQGSLPRPAELEVYLGETGIFCR
                     QLNKEAEPQPVGAGSEAPSQGTPLYMF"
     gene            complement(16136..16879)
                     /locus_tag="VFQ79_09355"
     CDS             complement(16136..16879)
                     /locus_tag="VFQ79_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0139905.1"
                     /translation="MKRGVCWRLMGSGTDTSFKALDLTRRYQSGQSELVVFAALNLDV
                     YRGERLALIGESGAGKSTLLHLLGGLDRPSEGTIFFETREISGLSDAELSNYRNREIG
                     FVWQTHYLLPEFTALENVMMPLLIRNVSQPEASVAATRMLEEVGLKERATHRAGELSG
                     GEQQRVALARALVGRPKALLADEPTGNLDFKTGEMIMDLLDELHRSHQLTSIYVTHNQ
                     KFAARCDRVLRLDKGELFSLDSSSQGLRE"
     gene            complement(17068..>17950)
                     /locus_tag="VFQ79_09360"
     CDS             complement(17068..>17950)
                     /locus_tag="VFQ79_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0139906.1"
                     /translation="IAKLIGSPPGYLGHRETHPLLSQEVINQYHTDRVKMGFILFDEI
                     EKASDALWNLLLGILDKATLTLGDNRKVDFSRAMIFMTSNLGAAEMNHYMSPKLGFNA
                     FEMAQKATNGSVDEKTSAKISRTGVEAARRKFTPEFINRLDKIVVFKPLGEQELRSVL
                     EIELRLVQQRIFNSVCDKPFVFTVTDAGKNYLLEEGTDMKYGARHLKRAIERLLVHPM
                     SNLIATEQVRGGDWIRVDFCPDTKTMTFSREAEALPIHQMAELIDNPVALPTGAVAAG
                     APYEVAKSQSARSSRRN"
BASE COUNT         3514 a         5357 c         5478 g         3601 t
ORIGIN      
        1 tcccgttcgg ttgtgaatac acgcgccgga ccggttttgc gatatacgcc gtcttcgtcg
       61 acgacgtccg ggtctatcgc cgtggcttta atcacagcgc cctcaggggc caggttgccg
      121 ccgacgaaac agaccgtgga agtgagcccc cgccggcggg ccgtggcagg atccatgatg
      181 acatcatccg gatcgacacc gtcgcgctct ttcaataccg tccgcaagcg cgcgcggcgt
      241 tcagaatgtt cccaccagtc gagcatggtt ccgagcgttt cccccgacgc ggtcaaggca
      301 tcgagctcca gcaacccggc gcgccgcaga tgcagcatca cctccggcac gccgcctgcg
      361 aggaacacct gaatggtagg gtggttgcgc ggcccgttcg gaaggacatc caccaggcgc
      421 ggaaccctcc ggttgaccgc ggcccaatcc tcgacagacg gacggcgcag agcgcctgaa
      481 taagcgatgg cgggcagatg cattaaaaga ttcgtcgaac cgccgaatgc cgcgtgcgtg
      541 accatcgcgt ttcgcacgga agcggacgtt agcagcttcg acatggtgag gccgcgcgat
      601 tcgagcgcga gcaccgcccg cgcggaacgg cgcgccatat cgagccagag cgggtggccg
      661 gacggcgcaa gtgcggaatg cgtcagcgac atgccgagcg cttctcccac cacctgggaa
      721 gtggcggccg tccctagaaa ctggcaccct cctcccggcg aagcgcaagc ccggcacccc
      781 atttcggccg cgtgctcgag cgtgatctgc ccgtgcgcgt agcgggcgcc gatggtttgg
      841 accaggcccg catcttcgcc ctcttcgggc aatagcgtaa cccctccggg gaccaggatg
      901 cacggcagct cgtgtatcgc cgccagtgcc atcatcatcg ccggcaagcc tttgtcacag
      961 gtcgccacgc cgatgacggc gctgcgcgtg ggaagcgacc ggatctgacg gcggaagatg
     1021 gtggcggcgt cgttccggta tggcaggctg tcgaacattc ctgtcgtgcc ttgcgtacgc
     1081 ccgtcgcatg ggtctgtaca ataggccgcg aagggcaccg catccagatc tttcaattcg
     1141 cgagccgcgg cttccatcag caggcccact tcccagtgcc cgctgtgata gccgagagcg
     1201 atgggagtcc cgtccggggc acggatgccg ccgtgcgtgc tcaggatcag aacttctttg
     1261 ccgccgagct ttgccggatc ccagcccatc ccggcgttca tggtccagcc gaaaaggtcg
     1321 ccggacggcc gcgtcagcag catctcctcc gttagcggga gcgcaccttc cggtccgctg
     1381 gttttcgagc ggatgtcata aatcgagggg tcgccggaat cgagcaccga ctcgacggat
     1441 atctgttgcc gtgccatcaa atgcctattc gatcaggctt tgatagcata ctgcaatgtc
     1501 ttctactcgc cgctgttttc tgggtgcgat gacggcggca tcctgccagc gggtggtggg
     1561 cgccaacgac agggtgcggg tcgggttcat cggttatggg ctgattggtg cgcagcacgt
     1621 attcgatttc aaaaatcaga aagactgcga tcctgcggcg atgtgcgatg tataccagcc
     1681 gcgtctggag cagggcgtag cggcctgcgg aggcgcggcc aagggttacc gcgattttcg
     1741 caagctgctg gacgacacgt ccatcgacgc cgtggtgatt tccacaccgg atcactggca
     1801 cgctccgatg gcgattctgg cgtgtgccgc cggcaaagat gtgtatgttg aaaagccgct
     1861 gacgcttttc gtccgcgagg ggcgctggct ggtaaccgcc gcccgccgtt acaaccgcat
     1921 cgttcaggtt ggcacccaac agcgtagcgg gcttcattac caggaagcgc tggggctgct
     1981 ccgaagtggc gtcatcggca ggattcacag cgtgcgcatg gcgtcctttc gcaatgtgat
     2041 gcccggtttc ggcaggccgc cggacagcac gccgccgagc gatctcaact acgatttgtg
     2101 gctcggcccc gccccgcggc gaacgtacaa cccgcatcgc gctttatacc atttccggtg
     2161 gttctgggat tactcgggcg ggcagatgac aaatctgggg gcacaccaga tcgacatcct
     2221 gcaatgggtc ctggaactga acgggcccac cgcggtttcg tcggcgggcg gacgattcgt
     2281 actggaggac gacggcgaaa cgccggacac acaggatgcg gttttcgagt accccggcct
     2341 caccgccgta tggtcgcacc gcgaagccag cgccgggcgt ggtgcgtcgg atctcgaatt
     2401 ctgcggaact aagggaagca tgactgtttt gcggtccggc ttcgaggttt tcgcggacgt
     2461 gaagagaaat cccgaggacg ctataccggt atttcagggc acgccggcgg gtggtccgaa
     2521 gcggagcgcc tcgccacgcg catactggac gacgccagtg aagaagccgg gttcctctgc
     2581 ccaacagctt gatctgcatg tccgcaactt cctcgattgc gtgaaatcgc ggcagaaacc
     2641 catcgcggat gtggaggagg ggcaccgcac ggctaccacg tgccacctgg cgaacatctc
     2701 tctgcgcatt ggccggaaga tccgctggga ccaggccagc gagcagatcc ccggcgacag
     2761 cgaagccagc gccatgttgg agcggcccta ccgcgagccc tgggccggaa tcatcgggag
     2821 cttgaggcta tgactcggcg cgagcttttg ctggcgggtt caagcgccgc cttcttgcgt
     2881 ggccagaacc ctcgcgtgcg gatcggcatc gcgacgacgt gttaccgggc tgcccggcgc
     2941 cccaaagaca ccatcgggtt cctggaattc gtgcattcgg ccggtggtgg cggcattcag
     3001 gcgagtctct cctccctgga ccctgtttat ctgacccggc tgcgccgccg cgcggaagag
     3061 ctcgatatgt acgtcgaggt gatggtggat cttccgcaca ccgacactac cccgttcgag
     3121 cggacggttc aagcggccaa atacgccgga gcgctttgca tgcgcgcggc gtgcctgagc
     3181 gggcggcggt acgaaacgtt tgacagcctc ggggaatgga aagagtttgt tgcccgttcg
     3241 cacagctccc tgcgccaggc tgcccggatc gttgagaagc accggatacc ggtagccgtc
     3301 gaaaatcaca aagactggac cgccgacgag ctcgaatcgc tcatgaagca gtacggcggc
     3361 gattatctgg gcgtgtgcct ggacaccggg aacaacatcg ccctgctgga cgatcccatg
     3421 gaggttgtcg aacggctcgc gcccaacgcg ttttcgacac acatcaaaga tatggcggtc
     3481 gacacttacc cggatgggtt tctgctatcg gaggtccccg tgggcgaagg catcctggac
     3541 atcaagcgga tcgtctccag tgttcaacgc gcgcgtccga agaccaggct aacgctcgaa
     3601 atgatcacca ggaatccact tcccataccg tgtctgacgg acaaatattg ggcgaccttt
     3661 cccgcgagca gacgtcctta tctggagcga accatgcgcc tggtgaagga gcgccggtcg
     3721 gacctgccgc ggttggacgg cttggacgac gatgcccaac acaagctcga agataccaac
     3781 gttacgcgct gtttgcgcta cttcgggaat gggtagaggc ctgcgtccgc atgaaacatg
     3841 cccgtaccat aaattgtaat attacatagt ataatgaaat cgatggcctt gaatctccag
     3901 gagtggcggc gacaaaacag ccttacccag gttgaggcgg cggcgctgct tggtgtctcg
     3961 cagccctatc tttcgctcct ggaaaagggt gtacgccctc taaccaagac cgtccgcgca
     4021 cggatgaaat cggtaaaccg agcacgtccg catatctctc tcgacgatcg tttccgcgag
     4081 caactgagtg ccttgggata tgcaccattc gcccatgtct ctcccgcccg cttgaagcct
     4141 gcgccagacg tcttcttgct ctccgttcta atgcagccag acgtagatgc gcgagttgta
     4201 gaggccttat cttgggtggt ccagacgtac gcggccaaaa tgaatttccc ttggctggtt
     4261 caacaggcaa aactggagaa cctacagaat cagatggggt ttttgtttcg cgtatcggac
     4321 tctaagagcc cagagcttgc cgttgcttta gatgcgctgg aaaaagcgcg gctcctcaaa
     4381 gaggccactc tctgttggga ttccatgccg tctgcaactc gcaagtggat gcgccaacat
     4441 cgttccgccc tggcgaggca ctggaatctg gtaacaacgc tgcaacagga acaatccatg
     4501 catgcggcgt agctgtcccg aagacaaacc cgcggaacca tggcggtctt tcctcaccga
     4561 actcgatcgg tttttggacg actcggtgga tttccattgt atcggcgggt tcgtcatcag
     4621 ccagttctac ggtctcgggc gcgagaccgc cgacctcgac attcttcgtg tcattccgaa
     4681 atacatagtc gaatgaatta ccgaggttgc gggccgcggg tcgcagcttc agaaaaggca
     4741 ccgggtctgc atcgactata ttcaagtggc gaactatcct gacagctacg agagccggct
     4801 ggttcgcgtg ttcccgaatt ggaagaatat tcggctttgg gcgctagagc ctcatgatct
     4861 tgcattgacc aagcttgaac gcagttctga acgtgatatc caggatgtaa tgtacctggc
     4921 gcaggcaggg ctcataaggc cagacatatt gttaaaacgc ttcgaaacag aaatggaacc
     4981 gtatgttgtt ggccgaacac ccacgtggca tcgcagcacg ctggacatgt ggatcgaatc
     5041 ctgttggccc aggtagtcca cgcctcgtcg aaataaggtg tgccacctga aattcccgga
     5101 agtccttcgc tttcatctgc gcttcccgca cttagtgcag ggagcccctc cgtgctaacg
     5161 tggttctttc cctatggacc accaaccgaa accgcttgtc gccgtaatca tgggcagcaa
     5221 gtccgattgg gacgttatgc ggcagacggc ggaaacgctc acacgtttcg atgtgcccca
     5281 cgaatgccgt gtgctctcgg cccatcgtac gccggcggca gcggcggagt atgtttcggg
     5341 cgccgagtcg cgcggtgttg aggtcgtgat cgccgccgcc ggcggagcgg cccacctggc
     5401 cggagtcgca gcggctcaca ctattctgcc ggtgctcggg gttccgatgg agagcgcgtc
     5461 tctcaaagga ctggattccc tgctttccac ggtgcagatg ccgggcggaa tccctgtcgg
     5521 aacgctcgct atcggcaagg cgggcgcgac gaatgcggcc ttgctggcgg tggcgatcct
     5581 cggcgtgaag cggcctgaac tccgcgacaa gctgcgccgt ttccgcgaag agcaggccaa
     5641 taagatcctt tcggaaacgc tggcttgatc ggcgataatc gtaagcggga gttgtcgtcc
     5701 ccgtgctgaa tcataatcat gaagatctgt tatctggacg cgttctcggg aatcagcggc
     5761 gatatgaccg tcgccgcgct gattgacgcg ggtgccgatg cgaagacgct gatcgagtgt
     5821 ctgaattcgc tcgaaacggg agcgtctttt cgagtcgaga aaacgaaacg ccggggcatc
     5881 gccgcttcga aattccacgt ggatgtcgag gacacccggg cgcaccggca tctgccgcag
     5941 attttgcgaa tgatcgaacg tgcgaagctg ccggacggcg cgcgtgaaca ggcaacggcc
     6001 atctttgaaa aactgggtga agccgaggca agcgtccatt gggttcccat cgagaaagtt
     6061 cacttccatg aagtgggcgc cgtggattcc atctgcgaca ttgtgggagc agcggtcggg
     6121 tttcgtctgc tggacgtgag ccaggtgtac tgctcgccgc tgaatgttgg tagtgggacg
     6181 gtccacacgg agcatggcgt gctccccgtg cccgcgcccg ctactgccaa gctgctttcc
     6241 ggaaaaccag tgtattcgcg gggaccggaa gtagaactga caacgcctac aggagcggcc
     6301 atcgccacaa cgctcgccgc cgggttcggt ccgatgcctc cgatgaggat ctcctcggtg
     6361 ggctacggcg ccggagatct ggaatttgcc gagcacgcga acgtgctgcg catcctggtt
     6421 ggtgaagcct cacaagcgac cgaggcaacc accgtagcag tgattgaggc gaacatcgac
     6481 gatgccagcc cgcagatgct gggctatgcg atggagcgat tgctcgaaaa cggcgctctc
     6541 gacgtaacca tgacaccgct gttcatgaag aagaaccggc cgggcactct gctgagcgtg
     6601 atcgcgcgtc cggaagaccg cgagcgcctc tgcggcctgg tattcgccga gacgccgaca
     6661 ctggggctgc gggtttatgc ggcggagcgg cgtgtcgaag aacgcaggat cgtggaagtg
     6721 gacactgcgc acggcaagat ccggatgaaa gtgtcgggtg cgggtttcgc cgcgcccgag
     6781 tacgaagact gccgcaggct tgcatcggaa aagggcattc cattgagggt ggtgctggcg
     6841 gacgccacca acgcatatct gaacaagaga tgaaatatta cctcacgact cccatctact
     6901 acgtcaacgc cgctccgcat atcgggcatg cctacaccac catcgttgcc gacctggtgc
     6961 ggcgctttaa acggatgcag ggctacgaag ccgttctgac gacgggctcc gacgagcatg
     7021 gtgtaaacgt ggaacgcgcc gcccaacgtg ccggcaagag cgcgaaggaa ttttgcgacg
     7081 tcatttccga agagttccgt gtccaatggg agcgcctggg gctgagtgtg gaccgctttc
     7141 agcgcactac cagtccccag cacgcgaagg tggtccagga ccttttcgag cgctgccgcc
     7201 ggaacggcta tgtctacaag ggctcctaca ccggccagta ttgcccgttc gacaacctgt
     7261 acgtgaacga tgccaagccc ggggacccgt gccccgattg cggccgtccg accgaaactg
     7321 tcacggaaga gaacttcttc ttcaaactgt cggcgttcgc cgaccgtctg ctcgatcttt
     7381 acgaaacgca gccggatttc atacagcccg ccacacggcg caatgaggtg atcgcgttcg
     7441 taaagcaggg gctgcaggat ctgtccatca ctcgaacgaa catcaagtgg ggcattccgg
     7501 tcgaggggga agcgccccac gttttctatg tctggttcga tgcgcttagc acctatatga
     7561 gcgcggtcga gggagaaggg ctctggcccg cggatcttca cctgatcggc aaagaaatcg
     7621 tgcggttcca cgccatctac tggcccgctt ttctgtgggc cgccgggctg gagcttccga
     7681 agcgcattta cgcgcacggc tggctgctgt ttgaaagcga caagatgtcg aagtcgcgcg
     7741 gcaatatcgt gcgggccatt cccatcagcc aggtgatagg tgtcgacgcg ctgcgctatt
     7801 ttctgctgcg cgaaattgtg ttcgggcagg atggcagctt cagctatgac gcgctggtgg
     7861 gccggtataa ctccgacctc gcgaacgggt tggggaatct tgcaagccgg accctgagta
     7921 tgattcatca gtatcgcggt ggtgctgtgc catcgggaac cccggcggcg tcttccgctg
     7981 tcgcgacgca ggcggcggat acaatcgcgg ctgtgatcgc ggcatttgaa aatttcgagt
     8041 tctcaaaagg gttggagacg ctctggtcgc tgctgtccgt cgtggaccgg ttcatcgtcg
     8101 aaaaagcacc gtgggttctc gccaagaagg aagacgaagc gtcacaggcg gttttgaatg
     8161 agacgctcta cacggccgcg gaagttctcc gcattgcgac ggtgctgctt gcgccggtgc
     8221 ttccggaatc cgccgcgcgc atctggcggc aactgggcat gacgcagccc ctggaggagg
     8281 ttcggtttgc cggtctgagc tggggacaac tcgccgcggg ccagatgatt ggcgaagcca
     8341 ccgccgtgtt tccacgcatc gacgccaagc cggccattga caagatgcgt gagctggaaa
     8401 agcaggagac ggcgcggcag gccgccttgt tcggaaagac cgcgccggaa acgcccgggg
     8461 cggcagcggc gccgcaaggc gacggtatga tcaccatcga cgacttttcc aaaatcgatc
     8521 tacgcgtcgg agaggtgcgt tcggccgagc gcgtgaaagg cgcggacaaa ctgctccaca
     8581 tgatgatcga tatcggagaa gaacggcccc gcagcatcgt cgctggaatc gcacttgctt
     8641 acacaccgga gcagatggtg ggcaggaagg tcgtcatcgt agcgaatttg cagccgcgca
     8701 aactccgcgg catcgaatcg caaggcatga ttgtagcggc gtcgctggcg gatggcgccc
     8761 cggtgttggc cgggtttctg gaagacgtgc ccatcggagc acgcctgaag tgagtcttac
     8821 cgatactcat tgccaccttg atgacgagaa gttcgattcc gaccgcgatg ccgccatcga
     8881 gcgggccttc gaagctggtg tgacgcaaat cgtcgccgtc ggaacaggcg atggacctcc
     8941 cgatctggag gccggtatcc ggctggccga cgcacatcaa aatgtgtatg ccacggtggg
     9001 agtgcacccg cacgacacga gcaaagccga tgacggcagc ctccgccggc tcgaggagtt
     9061 gttaaagcac gaaaaagtag tagcagtcgg ggaggttggc ctcgattatc actacgattt
     9121 ctcgccgcgc gaccggcagc gggcggtgtt cgtggaacag atgcgaatcg cgcgggatgc
     9181 gcggaagccc atcgtgattc acacacgcga ggcctgggac gacacgattg ccttgctcgc
     9241 cgagcattgg acgccatcag gccttggcgg catcatgcac tgctttacag gcggagcggc
     9301 acaggcggca cagtgtcttc agctcggctt tcatctgagc ttcgccggga tactgacttt
     9361 tcccaaagcg ttggagattc gcgaggcggc ggcgatgtgc ccgatggacc ggctgctggt
     9421 ggagaccgac gctccctatc ttgccccgat cccgcaccgg ggcaaacgta acgagcccgc
     9481 tttcgttgta catacggcac aggcgttggc cggcttgcgc ggactcaccc tcgaggaagt
     9541 cgcgcgggca accacgcata attttggccg gctctgtttg ccagagaatc gggacagcgg
     9601 gtacactgaa accattccat gggattcagc aagctagggc agtctttcac cgctagggaa
     9661 atcctggagc tggttcagga cgatctgcgg aaagtagaga gggagatccg tctcgaatcc
     9721 gtcgcctcgg tcgatgctat caccacgatc ggcaagtact tgcaggacgg cggcgggaag
     9781 cggctacgcc ccactctcct cttgctggca tgcaggcttt tcagcgagcc gcccgagagt
     9841 gcgatccgtc tcgccgcggt catcgagatc atccacacgg cgacactggt ccacgacgac
     9901 gtcatcgata tcgccaagac gcgccgcggc aggccctctc cgaacgtgat ttggggcaac
     9961 catacttccg tgctcgcggg ggactggttg tacatgcagg ctttccagat ggcccttcgc
    10021 gagcgcagtt tccatgtctt ggacctgctg atcggcctca cccagatgat ggtggaaggc
    10081 gaactgctgc aactggatcg catccggaaa atcgatatct cggaagcgga ttacatggag
    10141 ctggtcgatc gaaagacagc cagcttgttt tccgtggcgg cgcggctcgg ggcgattacc
    10201 gccggagcgg acgatgtttc cgaagcccgc ctgggcgaat acggatggag tctcggcatc
    10261 gcatttcaac ttgtggacga tgttctcgac tttacggcgc atgaatccgt gctgggaaaa
    10321 cccgttggaa acgatcttca ggaaggcaaa gtgacgctgc cgctcattta tgcgctggag
    10381 cgtacgactg gttccgagcg cgagctggtc gagaccgttc tagccgatgc gaactaccac
    10441 cgcgtgccgt tctcaaccat tctggagctt attgaacgcc acgatggcat ctcgcgagcc
    10501 aaagagcgcg cacaaatgtt cacggataaa gcgcgctcca ttgcaatgga gtttcccgaa
    10561 tcgccttatc agcgggcgct ggtggcccta acggacctcg tcaccgagcg ggattactga
    10621 gaaatagtga agaccaggcg tgaatggctg accggcgccg gctccctgtt gggagcggcg
    10681 gcggggggtg cattcgcgtc gcagaagcag cgcggccgcc ggcctcgtat tctgctccgc
    10741 agctcctggc agagcgtgaa catcggcgat atcgggcaca cgcccggagc gctctcgctg
    10801 ctaaacaagc acttccccga agccgggatc acattgtggc cggggcggct ggatcaagcg
    10861 tcgcgggacc tgctgacgca caactatccg gatctgaaga tcgtggaggg aaccgtgggg
    10921 gcggacggcc gtccaacgcc ggagctggcg cgtgcgtggg cggagaccgg cttgtgtctt
    10981 agcgggtccg gctcggggtt ccccgcgagc gcgcacgctg tcgcatttca caaggcgacc
    11041 ggcaagccgg tcggcgtgtt tggcgtcagc acagacccga tttcgggcat cggtgaggga
    11101 agagagccag agggcggcac gctgaaggag ctgcgcgcga aagcgctgcg gctgccgccc
    11161 ggacacctgc ccgccgatct gcgatacatc atcgatcgcg ctgcgttttt cttctgccgt
    11221 gacacgattt cgcgggacta tctgcgggcg cagaaggtaa agacgccgat tctcgagttc
    11281 gggcccgatg ctcagctcgg gatgcatatt cgcgacgatg cgaaggggct ggcgtacctg
    11341 aaagcaagcg ggctcgaaga ggggcgcttc atttgcgtca tcccgaggct ccgctacacg
    11401 ccgtattacc gcatccgcgg cacgccgcgc actccggcgg acgatatcag agatgctatc
    11461 aactcgcgca cgacggaagc ggaccatgcg aagctgcgcg agatgatcat ctcctacgtg
    11521 aagaacacgg ggaatcgcgt gctcgcgtgt ccggagatga cttatcagat cgagctggcg
    11581 aaggaggttc tggtcgatcc tctgccggag gcgatcagaa agagcgtggt gtggcgggac
    11641 agcttctggt tgcccgacga ggccgcgtct gtctatgcgc gcgcgcaggc ggtgatcagt
    11701 gtggaatgcc attctccgct gatcgcgctg cacaacggga caccggcgat gtacgtgcgg
    11761 cagcccaccg atacctgcaa gggccagatg tatcgcgatt tcggcgctgg gagatggatg
    11821 ttcgagatcg aggaaaccag cggcggcgag ttatggtcgc gggtggcgga gattgtgaaa
    11881 gatccgggaa aggcacgggt tcgtgtccgg tcgatcctgt ccggggtcga ggaccggcag
    11941 aagcggatgg ttgctgctgt gcgggcagcc tgctgagccg cctcacaacg tgaattcgat
    12001 tccctgtgcc aaaggaagat cactggacca gttgatggta accgtctggc ggcgcatgta
    12061 ggttttccag gcgtcactac ccgcctcgcg ccctccgcct gtttcttttt cgccgccgaa
    12121 ggctcctccg atttccgcgc cgctggtgcc gatattcacg ttggcaatac cgcaatcgct
    12181 gccccgggcg ctgagaaacg tctcggcgga gatcaggttt gtggtgaaaa tcgatgagga
    12241 aagcccttgc ggcacattgt tgtgccactc gatggcttca tcaagggtgt cgaattcaat
    12301 caggtacaac accggcgcaa agagttcttc cttcagaatc ggcatctccg gccgcgcttt
    12361 gacgagcgtg ggtttgacgt agcagcctcc ggcccggtcc agcgctttgc cgccgaaaat
    12421 cacttcgccg ccctgctccc gggcttgcga gaggccttcc atcatatcct tcacggcggc
    12481 gcggttgatc agcgggccca tcaacgtatc cccgcgcaag ggatccccga tgcggatctg
    12541 acggtagacg ttcagaagca tcccggtcag ttttggcgct atacctttct gcagaaagag
    12601 ccgccggatg gttgtgcagc gctggcccgc ggttccaacg gcgccgaaaa gcaccgcccg
    12661 taaggcgagg tcgaggtctg cgtcgtccat cacgatcacg ccgttgttgc cgcccagttc
    12721 gagaagtgtg cgtcccaggc gattgcccac tgcctcctga acgcggtacc ccatcgaggt
    12781 agagcccgtc gcgctgatca gtgggatccg ccggtccttc gacatgcgtt cgccgacaac
    12841 cgagctctta ccgattacga gattgaatac tccccgccag ccgtgccgct cgagcacgcg
    12901 gttgcagata ttctgaacgg cgatggcaca gagcggcgtt tccgatgacg gcttccagac
    12961 gacacaatcg ccgcaaacgg cggcgatcat cgcattccac gcccaaaccg ctaccgggaa
    13021 gttgaaggag gtaatgacgc ccaccggccc cagcggatgc cattgctcgt acatccggtg
    13081 gccgggccgc tcgctgtgca tggagagtcc ataaagctgg cgtgagagac cggcggcgaa
    13141 atcggcgatg tcgatcatct cctgcacttc ccccttgccc tccgggagaa tcttgcccat
    13201 ttcgagcgac accagcgcgc ccagatcgtc tttcacccgg cgcagctcgc cggcaatatc
    13261 gcggatgatg tcgccacgcc gtggggccgg cagcaggcgc caatcgtgaa aggcatcggc
    13321 tgcgtgccat accacctcgt cgtactcttc ttcggcggcc atccggatcc gcgcgatttc
    13381 cttgccgtcg tgggggcttg aggaaatgag ttcgcctcct tcgggttcgg gaatccaatc
    13441 gccccaacac gccccggagt ggattgcttc gatcccaagc ctctggagaa gatcgcggcc
    13501 gggcggcgaa ccggacgcct ccccttgtat ttcgtttacg tcatcagagc cagccatcga
    13561 agagcctccg catccgcaag ccaatgcaaa aagggccgga tttctccggc ccctccgaat
    13621 ttcactgtac cgctccgtca gaacatgtac aatggcgtgc cctgggacgg cgcttcgctg
    13681 ccggcgccga ccggctgagg ctcggcttcc ttgttcagtt gccggcagaa gataccggtt
    13741 tcgccaagat agacttccaa ctcggcggga cggggcagcg acccttgtat caaggcttcc
    13801 gataacggat cctcgatgta cttctgaagc gcgcgccgca acggacgtgc gccgtagcta
    13861 cggtcggcac aggtcttttc cagaatgtac cgggccgcat cgggcgccag ccggatagta
    13921 atctgtttgt ccaccaggtt ggagttgatc tgaagcacca tcaggtcgat gatcttaatc
    13981 aagtcatcgt ccgtcaacga ggtaaagagg atcacttcgt ccaggcggtt caggaattcg
    14041 ggattgaagg cccgcttcac ttcgctcagc acctggtctt caattttgct gggaatccct
    14101 tgctcctgcg gagccgagaa acccatcggg gacttcttat ccaggaatcg cgcccccagg
    14161 ttcgaggtca tgatgataat cgtgttcttg aaatcgacgg tgttgccgag accgtcggtc
    14221 aactggccgt cttcaaacac ctggagcaga atgttgtaaa catccgggtg cgccttctct
    14281 atctcatcga gcagaatcac cgagtagggc gaccgcttca cacgttccgt caactgcccg
    14341 ccttcctcgt agccgacata tcccggagga gaaccgatca acttcgaaac ggagtgtttc
    14401 tccatgaact ccgacatgtc aaagcggatc agggactttt cgctcccgaa gagaaactcg
    14461 gccagcgcgc gcgcgacttc ggtctttccg acgccggttg gtcccagaaa caggaaagaa
    14521 cccgcaggcc gtttcggaga cttcaagccc gcgcgggagc ggcggatagc gcgggaaaga
    14581 gccgaaaggg ccttttcctg gctgatgacc cgcttgtgca actcctcctc gatccggagc
    14641 aacttggcaa cttcctcttc gcggatagcc gtcatcggga cgccggtcca gcgggccacc
    14701 acgtcctcga tatcgtcttt tccgacgacg ccggttgaag tgtcgtccag attgtacttc
    14761 tcccgcagca ggcgtagatt ctcgcgttcc ttgcgttcct cgtccgagta gaagcgcgcc
    14821 ttttcgaatt cgtggttggc gatggcgttt tccatccggt gcacgatgaa cttgattcgc
    14881 ttctgaacat cggctacctc gcctgggagc gtagtctgcc ggagctttac tctcgcgccc
    14941 gcttcgtcga tcaaatcgat ggccttgtcg ggcaggtaac gatcgggaat gaaccggctg
    15001 gaagcgtaca cggcggattc gatcgagtcg tccgtgtaag cgacagcgtg gaatttctcg
    15061 tagcgctcct tgatgccgaa caggatccgc acggcatccg cttcggttgg aggcggcacc
    15121 ttgacagcct gaaaacgccg ctcgagcgag cggtcttttt caatcgactt gcggtattcg
    15181 ccaggggttg ttgcgcctat gcattgtatt tcgccccgcg acagagcggg cttcaggatg
    15241 ttggcggcat ccagcgatcc ttccgccgat cccgcgccga ccagggtatg caactcgtcg
    15301 atgaagatga tggcgttctg attctccatc aactccttca tgatggtctt cagccgttct
    15361 tcaaactggc cgcgatattt agttccggcc acaatcagcg agaggtcgag cgccagaatg
    15421 cgcttgtcgg aaagaaagga gggtacatcg ccatctgcga tccgctgcgc caatccttcg
    15481 acaatcgccg tcttgccgac gccgggctcg ccaatgagca cgggattgtt tttcgtgcgc
    15541 cggcacagga tctgtacgac gcgctcgagt tcgtaatccc ggccgatgag cgggtccaac
    15601 atgccgtcca tggccgcctg ggtcaggtcg cggctgaatt cggagagcag ggaactctcc
    15661 ttggggcggc tcgatgccat tttttccgag gaggaccgcg caatttcctc acgaacctgg
    15721 gaaagcctca acccccgttc atgcagaatc tccgccgcaa agcacttttc ctcgcgcaag
    15781 aggcccagca acagatgctc cgtgccgatg tgcttgtggt tcagccgctc ggcttcttcc
    15841 gccccataag cgagcacgcg tttgcattcg tggctcagag gcagatcgac ggacgtcgag
    15901 accttttccc gaatcgtggt gtgcgcctcg atctgcttgc gaatggattc gatggcagca
    15961 acggagcgca ggaaacgatt cgccagggcc ttgtcttcgc gcaaaaggcc gagcagcaag
    16021 tgctcggtct caatatacgg gctaccgaac tggctggctt cgtacctcgc gaagaaaatc
    16081 accctgcgcg ccttctcggt atatcgttca aacatagtct taccgtctac tctttctatt
    16141 ctctcaagcc ttggctactc gagtcgagag aaaacagttc acccttgtcc agcctcaaga
    16201 cgcgatcgca ccgcgccgcg aacttctggt tgtgagtaac atagatggac gtgagctggt
    16261 gtgagcgatg caactcgtcc agcaggtcca tgatcatttc gcccgttttg aaatcgagat
    16321 tgccggtcgg ctcatcggcc agcagcgcct tggggcgccc gaccagcgcc cgggcgagcg
    16381 cgacgcgttg ttgctctcca cccgacaatt caccggcccg gtgagtcgcc ctttctttca
    16441 accccacttc ctccagcatc cgggtagccg caaccgaagc ttccggctgc gagacattcc
    16501 ggatgagcaa aggcatcatc acattctcga gagctgtgaa ctccggcagc agataatgag
    16561 tctgccagac aaacccgatt tcccgattgc gatagttcga cagttcggca tccgagagcc
    16621 ccgagatctc cctggtttcg aagaatattg taccctccga gggccgatcg agccctccga
    16681 gcagatgtaa aagagtggac ttgcccgctc cggattctcc aatcaaagcc agccgctctc
    16741 ccctgtacac atccaggttc aaggcggcaa aaaccaccag ttcactctgc cccgactgat
    16801 aacgccgggt aaggtcgagt gctttgaaac tagtatcggt gccggaaccc attaaacgcc
    16861 agcagacccc tcgcttcacg ctatcatgaa ccggctgctg ctttggcggc cggccccgcg
    16921 cctgcccatg ggacaggtga cctgaagata agcctctagg aattcgatgc tggaacctgg
    16981 cttgaggttg ccgtctgggt ttgtgggcgt ccggagacgc ccgccggaga ggactatctg
    17041 tgtgtatccg tgtccatccg tggctaacta gttgcgccgc gacgatctcg cgctctgcga
    17101 cttggctact tcgtacggcg cgccggccgc gactgcgcca gtgggcagcg caaccggatt
    17161 gtcgatgagt tccgccatct ggtgaatcgg cagcgcttcc gcttcgcggg agaaggtcat
    17221 ggttttcgtg tcgggacaga agtctacgcg aatccaatcc cccccgcgga cctgttctgt
    17281 cgcaatcagg ttcgacatgg gatgcacgag cagccgttcg atcgctctct tcaaatggcg
    17341 cgcgccatac ttcatgtcgg taccttcctc cagcaaatag tttttgccgg cgtccgtgac
    17401 agtgaacacg aacggcttgt cacaaacgga attgaagatg cgctgttgca cgagccgcaa
    17461 ctcgatctcc agcacgctgc gaagttcctg ctctcccagc ggcttgaata ccacgatctt
    17521 atcgagccgg ttgatgaact ccggcgtgaa ttttcggcgt gccgcctcga cacccgttcg
    17581 tgagatcttg gccgaagtct tctcatccac tgaaccgttc gtggctttct gtgccatttc
    17641 aaacgcgttg aatcccagtt tgggactcat gtagtggttc atctccgcgg cgccgagatt
    17701 gctggtcatg aaaatcatgg cgcgggagaa atcgactttg cggttgtccc cgagcgtcag
    17761 cgtcgcttta tcaaggatgc ccaggagaag gttccacagc gcgtcgctgg ctttttcgat
    17821 ctcgtcgaaa aggatgaagc ccatcttcac ccggtccgtg tggtactggt tgattacttc
    17881 ctggctcagc aagggatgtg tttcacgatg ccccagatat cccggcgggg atccaataag
    17941 tttggcgatc
//
LOCUS       DASTMK010000211        29277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_330152, whole genome shotgun sequence.
ACCESSION   DASTMK010000211 DASTMK010000000
VERSION     DASTMK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 29277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29277
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330152"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFQ79_09365"
     CDS             complement(<1..475)
                     /locus_tag="VFQ79_09365"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0139907.1"
                     /translation="MCPNVQTDLQSPYRTLIEKLREQASTIARLSSGLDEAALSCRVV
                     EGKWSLIELVCHLWRVQRVFECRVIQMLESDNPQVEPYDPDQDPEFERLLAIGPARVK
                     EGFAEEREKLLSKLEALDAAAWHRPGRHREYLHYNVHFQIEYMVHHEAHHIYQMFQ"
     gene            548..1522
                     /locus_tag="VFQ79_09370"
     CDS             548..1522
                     /locus_tag="VFQ79_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEU0139908.1"
                     /translation="MLNTKMLLVLGLAVSSSLFAAQKKRVAVLDFDYATVSSHVAAIF
                     GTNQDVGKGISDILVDRLVKSGTYDVVERKAISKIIAEQNFSNSDRADASTAAKLGRV
                     LGVDAIIIGSITQFGRDDKTTNIGGQAVGGRLGRYGLGGIGRRQAKAVVGINARMIST
                     DTALILATAAGTGESQRSGTALLGSGGSAANHAGGAVDMSSSNFADTILGEAVHAAVG
                     QLATQLEQQTSRLPQNTVTVQGLVADVAGDTVVLNVGTKAGVKVGDRLRVERPVREVR
                     DPSTGKVIRRITDQVGEVAVTEADEVSSVGKFTGSAPAKVGDVVSSAQ"
     gene            1529..3121
                     /locus_tag="VFQ79_09375"
     CDS             1529..3121
                     /locus_tag="VFQ79_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0139909.1"
                     /translation="MEIGQRIGDYEIAGILGAGGMGKVYKVRNVISDRNEAMKVLLPS
                     LKADPELGDRFLREIRVQASLDHPNIAALHTAQSIGDQLVMIMEFVEGVSLDKLIQRG
                     PVPLDKALNYTAQVLSALAYAHARGVVHRDIKPQNIMLAEGGVVKLMDFGIARLAADR
                     RLTQTGRTVGSLYYMSPEQIKGALDLDGRADLYSLGVTLYEMVTGKKPFVGDSDFSIM
                     AAHLEKAPVPPVQVDPTLPPELNDVILMSIAKDPSQRFQTAEAFGNALAAVRGAVAAL
                     GATPTIGSTQQQPPAAAPAAATRSRRGLYMAVGSVLTLAVLIGAATQLPKFFRTSADG
                     PAADAIVEPAQTPLRPEPVAQTPEISTQTNAPQTAVEPAPAVEPPAVKMPDAAPKRVE
                     RQATTVAVPAPRASSPVPAAVNTPTQSVSPTAVAPTEPAGERSVQPAPDQAALQKLEQ
                     RMMLMAARVAGVSATLTRMEREMAAQGLGMRSDFVSAQRRLELHMDQGEAALKKGDVA
                     AGGRSLDAAERELETLEKALGR"
     gene            3225..4025
                     /locus_tag="VFQ79_09380"
     CDS             3225..4025
                     /locus_tag="VFQ79_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0139910.1"
                     /translation="MIENLRPGASAKRARVVLFDFDGTLSLIRSGWVEVMVPMMVEVL
                     AELKSGESEAELKQVVDEFVARLTGKQTIYQMIELENQVRARGGEPEDPLVYKKWYLD
                     RLWDRIRGRVDELKKGHCAPEKYLVPGSLELLEALRARGLKLYLASGTDESYMKREAE
                     LLGVTPYFNGVYGALDDYKSFSKAILIQRIIGSAECAGDEFLGFGDGYVEIENIKQVG
                     GVAVGVATDEPECERIDEWKRNRLAGVGADWIVPNYRSSDRLMEALFQ"
     gene            4033..5001
                     /locus_tag="VFQ79_09385"
     CDS             4033..5001
                     /locus_tag="VFQ79_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139911.1"
                     /translation="MPSRYPLFDRSRLLIKPLSERIHDLHVEHWLGLADPAPPFSHPA
                     LPDVAARTVCAREKGAARILMMGAHVLRAGVSRHLIDLMERGFIDHIAMNGAGAIHDY
                     ELARIGGTTESVARYIRTGEFGLWWETGELNEWITGAASAAEGLGEHIGKRLEASSFR
                     YRDVSVLAAAWRLGVPVTIHVGIGYDILHEHPNCDGAALGAASYTDFLIFARSVERLE
                     GGVLLNFGSAIMGPEVYLKALSMARNVAGQEKRVIRDFTTAVFDLVPIEGDIHHELTK
                     RDPGYFFRPHKTILVRTVADGGTSFYFCGDHRATVPALRNALLEAA"
     gene            4998..5987
                     /locus_tag="VFQ79_09390"
     CDS             4998..5987
                     /locus_tag="VFQ79_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0139912.1"
                     /translation="MTAERILAAFPRLSALVVGDICLDRWCTYDPAESDPSRETGIPR
                     IGVVSTQITPGAGGTVANNLAALGAGRVAVLGVIGDDGFGFELSRALRSRGIEPDLLI
                     TTADVQTFTYTKIINGMSGVEDRPRLDFINTRPLAPDVERVVIERLNASAGEFDVIFV
                     SDQAETNAGGVVTEGVRQWLAQWRTAYPDKIVWVDSRMRSDRFRKAIVKPNLQEAEEC
                     CRRLFSRVDFEALRRHIDSDLLFVTHGERGVLVVEPGSESWVEARPVENPVDICGAGD
                     SFSAGAGLALAATRLPVAAARIGNLVASVTIMKKGTGTASPDEVLAAEKRLQV"
     gene            5984..6829
                     /locus_tag="VFQ79_09395"
     CDS             5984..6829
                     /locus_tag="VFQ79_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0139913.1"
                     /translation="MRILAVDIGGTKFSMAAFENGRITSRFTLPTDRDGGPQWMVQQI
                     ARTGQEWQRDGRFERIGIGFGGPVLFDRQQIALSTHVGGWSGFPLPEVLEREFGTPAV
                     MDNDANVGALGEWRYGAGAGCDPLFYMTLSTGIGGGILVGGRLLRGADGYAGEIGHLT
                     IRPGGPECLCGARGCFERMCCGLWLERDHGRPPDELLRDTGFVREYVVDLALGLKACI
                     MLLNPARIVIGGGISKAGEALFRPLRAELGRQITAWSRARVEVVPAALGDDSVLYGAL
                     CLAQN"
     gene            6855..7412
                     /locus_tag="VFQ79_09400"
     CDS             6855..7412
                     /locus_tag="VFQ79_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0139914.1"
                     /translation="MNSAELYKNELMETVGKIDTGKVQTAIEWFEEARDKGRHIFVCG
                     NGGSASTASHFACDIVKGASYNRDKRFRILALTDSLPTLTAYSNDVSYDCVFAEQLKN
                     FAEPGDLVMAISGSGNSPNVVCAVEYANSIGCKTIALTGRDGGRLGALAGLNIQVPVQ
                     HMGRIEDAHLIVCHMIGYHFMDYSG"
     gene            7563..9335
                     /locus_tag="VFQ79_09405"
     CDS             7563..9335
                     /locus_tag="VFQ79_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0139915.1"
                     /translation="MARGPAPSLAGVFVWELPDHSVTVQLPARVISELAGSPEGADLR
                     QSAGLQDLGGAILGSIEETAGVALLVEDCLSLPFFTGSEGRFAEDRNKDRFRDVLGTW
                     APGPDRRVFALGIYRRVLGQLVPSAEDVALYSSQFSGAPAILLLISARNSHPREASLF
                     VWRANAFVVAGSRFPLDGTALLAFSGAKHTPPSPLAVAPPGPQAVRNNGVAEVQQPVA
                     AVHPALAASVVARPVSAPGVSVRRWILPVVLGLLLLMVLTLGGLVARLYWPANVETVQ
                     SGGQLDLRAMRYPDSIFLTWNRTAPVLSSATGGRLAITDGEYHRELQLEKQELLSGSV
                     SYVPAGNDLHFRLDVFMSPDRTISESVRVIGGLFSELRRILPPIGTPPPRQEVSRPPG
                     SATVSTQEPRQFEGPPSLASSPREVSRGSSDRDASNRTVSMPQPPALPQRMVPDLTTI
                     SAVQRPSILPDAPPPPERSVPGAQQSVLSSQPLGTSGSAQPAKPVVSPPVPPVATRKV
                     MPSIPGSVRYLAENAGEVEVRVHVDARGNVTNAALVRAEKSAGIFLSRYALAAAYQWE
                     FRPAMAGGKPVNGEVLIRFQFASR"
     gene            complement(9341..10372)
                     /locus_tag="VFQ79_09410"
     CDS             complement(9341..10372)
                     /locus_tag="VFQ79_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0139916.1"
                     /translation="MRVAQLVAPHRFNLVSGQIENPGPGEVQVRVKMVGVCGSDLHNF
                     SEGSVGDAPALFPMVLGHEPAGVVVGTGPGVSGWSPGDRAALEPAIYCYHCEYCRSGR
                     HNVCNHIRFLSQPGDPGFFRDLVNLPVANLLPLAPNLDLGEATLFEPLAVTLHSMKLA
                     EPRLGDTAAVFGAGPIGLLTIAILKLSGVRVWAVEPVAHRRELAIRMGADATIDPSAV
                     DTVRQILADTGQRGVDFAIDCAAKGGSLDQAIGAIRRAGRVVVTGIPSEVRVEFDFHT
                     LRRKEGTLLSVRRSNHESDQALDLIKNHRALFAPMLTHTRPIEDIEKAFSLLERYDDG
                     VGKLIISLG"
     gene            complement(10377..10856)
                     /locus_tag="VFQ79_09415"
     CDS             complement(10377..10856)
                     /locus_tag="VFQ79_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139917.1"
                     /translation="MDNDRKYKQRGYMDSDRDRGGPSREERPRNQGPRPPIDVTGPRL
                     PRLVQSVTAYRCFNCSTALPADTDFKGLCPKCNMALHCCKQCAHFEPSTRFQCLKPIP
                     IRIAQKDQRNECELFKPRVTVARDVAPSASVGVAPSGPPAPRNADDARAAFDSLFKK"
     gene            complement(11135..13684)
                     /gene="glgP"
                     /locus_tag="VFQ79_09420"
     CDS             complement(11135..13684)
                     /gene="glgP"
                     /locus_tag="VFQ79_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU0139918.1"
                     /translation="MPFHIHRFKEFLVRPALPDTLSRLSQLAHNLMWNWDLTIRQVFR
                     RLDPALWTGCGHNPVLMLGRLPQAVLEKAGKDPRYLALYRRACERYDSYMDQEAPARD
                     RLVAYFSMEYGLAECLPIYSGGLGVLSGDHLKAASDAGIHLVGIGLLYQKGYLQQVLN
                     PDGWQQERYPTNDFYTLPVSPVINDKGEDLLVQVGLPHGPVYVKVWQINVGRVKLYLL
                     DTNIPENERPDDRDITDQLYGGDIHTRIRQEIVLGIGGLRALKALGLEPAAFHMNEGH
                     SAFLAIERIRVLIKEHNLTFEEALTASRNNNIFTTHTSVPAGIDLFEPGLMYDYFHGY
                     CNEGGIPFEGFLALGGRNGFDPQERFSMAIAAIKTSAYRNAVSRLHREVSQEMWQDLW
                     PELPTWEVPITSITNGVHLPSWLNTDLAMIYDQYLQPDWHERFHEPQTWDLVQDIPNQ
                     ELWEAHRRRKRQLIAFVRDRVTASAVARKASAPELRRQAEVLDPDAFTIGFARRFATY
                     KRATLLLRDTKRLKKILTNPDMPVQIVIAGKAHPKDHPGKALIREIVQLSRDPELYKR
                     LVFIEDYGIQVARELVQGVDLWLNNPRRGEEACGTSGMKAGINGILNLSVLDGWYDEA
                     YETSGGWAIGDREDYSEDQDEIHASAIYSLLENEIIPMYYRDREEGVPAEWMRRVKLS
                     LRNVSPQYSFQRMLEEYASQLYTPAQRAFLELQQGGFDKVRERVQWNAEVVRAWDHIR
                     FIAMGPEPEGVVVSGRPLPFRTTIDLAGLKPDDIRVEAVVGRVNGSGQLEETEVLALP
                     AVEQQGSAFVFAKDYIPTHTGRLGYSFRISPNHYEDPLTRPCNSLIKWAVE"
     gene            complement(13775..14269)
                     /locus_tag="VFQ79_09425"
     CDS             complement(13775..14269)
                     /locus_tag="VFQ79_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139919.1"
                     /translation="MSGLYAEPPRSIEVFGSAGIVKFGQDEGWGGSGAVYGGSVMLPV
                     LPKLAIDVNIVTARDDTGRVAERSEYSERRTLISPSLLYRFGTERAYGFVGGGAGAVF
                     QRSRAVTPFFLYDQSETFWSVHWRGGGVAALGARLLIRAELFSVHHYILPTIGAKAGV
                     GVRF"
     gene            14469..14972
                     /locus_tag="VFQ79_09430"
     CDS             14469..14972
                     /locus_tag="VFQ79_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU0139920.1"
                     /translation="MKLSAQEEYGLRCLLFMARHGDGHSLSIPEISRAEGLSVPNVAK
                     LMRLLRLGGFVASARGQSGGYTLSRPASQITVAEVVELLGGRVFSRNFCQRHAGIEPV
                     CTHIEDCSIRCLWSTLQTMVEGVLAKTTLLDLVVSEADMAAFLGSNGSAKTQSPELVP
                     EHTIAGQ"
     gene            15154..16377
                     /locus_tag="VFQ79_09435"
     CDS             15154..16377
                     /locus_tag="VFQ79_09435"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0139921.1"
                     /translation="MTSKHLEPSAHFNVLTESRIQTDGLFSLLDPEALYERPIPERHR
                     IIFYLGHLEAFDWNLIGRTGLGLESFHPQYDHLFAFGIDPPEGQLPNDQPPDWPSIEE
                     VLDYNRQVRRKLDEAWDSASPELRHVAIEHRWMHAETLVYIFHNLEYSRKKQGPSREP
                     AASPILENSLIDIPAGFATLGRKKEEGFGWDNEFEEHKVHVPAFRIRRHKVANGEYLQ
                     FVNAGGEAPFYWTRRGGEWHYRGMFNEIPLPHEWPVYVTFQQAVEYSRWKGVSLPTEA
                     QWHRAAFGTAAGEERLYPWGDAPPDASRGNFDFDRWDPVPVDANPCGESAFGVQQTVG
                     NGWEWTSTVFGPFPGFSPRSYYPGYSANFFDGQHYVLKGGSPRTAAALLRRSFRNWFR
                     PRYPYIYATFRCVEE"
     gene            16381..17355
                     /gene="egtD"
                     /locus_tag="VFQ79_09440"
     CDS             16381..17355
                     /gene="egtD"
                     /locus_tag="VFQ79_09440"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEU0139922.1"
                     /translation="MLARAFPANDPLLEFANDVTLGLTQEKQRELPSKYLYDELGSVL
                     FEAITLLPEYGLTRADERVIRRSAPEIGRYFGGALIAELGSGSGKKTRHILNACLKGP
                     SLTYAPIDLSPAALAACAGQLGNGAIEVHPIATSYLDGIGRAAALRQRKQKLLVLFLG
                     STIGNFDRNGAVEFLRKIGGCLLPGDGLLLGTDLEKPIPEMLVAYDDPTGVTAAFNLN
                     LLGRINRELGGDFDLRMFQHEARYDVEHRRIEMHLRSRADQTVTIPGAQLRVSFVCGE
                     TIWTEASHKFALAELPRMAAEAGFVHQQHWIDEEWPFAESLWMRAAGP"
     gene            17357..18085
                     /locus_tag="VFQ79_09445"
     CDS             17357..18085
                     /locus_tag="VFQ79_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0139923.1"
                     /translation="MRIPVEFRQVSFEIGGKTVLRDFSLALSEGETVALLGRSGSGKT
                     TALKLVNALLMPASGEVLVEGRATTAWDPIRLRRRIGYVIQETGLFPHFTVAGNVGLV
                     PRMEGWPEELINKRVDELLEIVGLAPREFGPRYPRELSGGQRQRVGVARALAADPPLL
                     LFDEPFGALDPVTRAEVQAHFLHLRNSLRKTALFVTHDVREALHIGNRIVLLKDGSID
                     LITTPAGFQTAGTPEAQAFLACLS"
     gene            18094..18711
                     /locus_tag="VFQ79_09450"
     CDS             18094..18711
                     /locus_tag="VFQ79_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0139924.1"
                     /translation="MRSGLAGDIVHLTLEHLMLVVTAVTVAVAIGVPAGVLLVRRAKW
                     RRWLLGFTNIVQTIPSLALFGFLIPIPAIGGIGRRTAIVALILYALLPVIRNTLVGIL
                     GIDPAVRESAVAMGMTPRQLLWKVEMPLAARTILAGVRVATVTTIGTATIGAAIGAGG
                     LGVFIFRGIASVDTTLILAGALPAAALALLFDGGLGWIEKRLAVV"
     gene            18681..19553
                     /locus_tag="VFQ79_09455"
     CDS             18681..19553
                     /locus_tag="VFQ79_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0139925.1"
                     /translation="MDRKTVGCCVISLLILAACGGGNRIVVGSKNFTEQVILGEIVAQ
                     HLERRLGERTERRLNLGGTLLAHQALLSGQIDVYPEYSGTALTAILNRDAAADAGAVY
                     QIVGTAYRKLGLEWLAPLGFNNSFAMVIPGELSRRQNLSTLSEAVRWQHAWRLGVGYE
                     FEGRPDGLPALLRTYKLQVSGQPRSMDLGLLYQALERGQVDMIAANSTDGLLSKMDTA
                     VLADDRGAFPPYEAGLVVRSETLARVPKVREALAELSGKISTGVMQRLNYRVDVDHEA
                     VGAVAADFLREAGL"
     gene            19620..20969
                     /locus_tag="VFQ79_09460"
     CDS             19620..20969
                     /locus_tag="VFQ79_09460"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEU0139926.1"
                     /translation="MHKFFLLLLLAASMPAADLKVGVARIKITPDGPIWMSGYASRDK
                     PSQGVVQELWAKALAFDDGRGRVVIVTTDLIGLPRSVSDAVAAQVQKQHGLERGRLLL
                     NSSHTHTGPVVRPNLNVMYDLDDEGDRKLKEYSQQLTDRLISVVGAALGDLAPARVAL
                     GHGNAGFAINRREPTATGVKIGVNPAGPVDHDVPVLKITSPDGKTRAILYGYACHNTT
                     LGGDFYQITGDFAGYSQAEIEKAHPGATALFLQLCAGDQNPNPRGTLDLAEQHGKALA
                     SEVSRVLQGSMKQVRPPLRFAYRATELEFAPHTRQTFEEQLKSSNRFAVRRAKLMLRA
                     YDEGRPVRRVPYPVQAVRFGKDLTLVALGGEVVVDYALRTKKEYGTSEGLVVAAYSND
                     VMCYIPSLRVLKEGGYEAVDSMIYYGQPGPFNEQVEEMVFDAIHDVMKRVGRRPEKR"
     gene            20970..23135
                     /locus_tag="VFQ79_09465"
     CDS             20970..23135
                     /locus_tag="VFQ79_09465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0139927.1"
                     /translation="MAAAATALLGSAFTIAACFGAGFALFPKLPLARSIRFALGAALL
                     STAVFLLLLAGAGGRTPFAVLGAGAIAAGAFLKGRISKRESAPWPLAAKAGAAFLLGA
                     YGALYLVHAMAPETAPDAITYHLGLVAEALRLGRFPNRIGFFEMLPQGAGMLFAHAFA
                     FGAHSAAKLVHFAFLVLTFPLLLRVGGKLGLHWSVSAAAALVYVLTPVVAVSGTSAYN
                     DAALVFCVLAVFYLLLCWREARDWRLLVCAGLIAGFCYAIKPTGAVVPVAGFVWIAIA
                     ARSWRSNLVFAAAAVLMCAPWVVRSAVLTGNPIAPLGNRLFPNPHFHITTEDRLSATL
                     REYGGVNAAAIPQEITIRGGKLQGLLGPIYLLAPLAFLAMRRRNGLTLAAALLVMAAP
                     WLLNTGTRFLMPALPFLLLLLFMALPRRALVPVVLVHALLSWPAAVSAYSDPSAWILR
                     GFPLKAAFRITPEREYLRERVGGFALAEALHGMTEPGERVLDLFGIPWAYSHFVPVGP
                     WHSATGDRLSTALQLAANPAALDVIVAEWPEQRISGVRFRVGQPFDTVWAIHEIQLRN
                     GSRILRPVRSWFFSANPNPWEILFAFDRNVASAWQSWKHMRPGMFIEIELAEPQRATG
                     ATMLAVRSDANRAIEVDLLTPDAAWQPVPHRLETRPPVDLTAAALALVRSSNIRYIVV
                     QAGGGGETGSLGRRLFERTRSGELDEMASERELRILRVP"
     gene            23561..27862
                     /gene="rpoB"
                     /locus_tag="VFQ79_09470"
     CDS             23561..27862
                     /gene="rpoB"
                     /locus_tag="VFQ79_09470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU0139928.1"
                     /translation="MENPANGAIRERVDFSKIKTSIPIPNLIDVQKKSYERFLQMELL
                     PEERDDTGLQSVFTSVFPISDFRGLSQLDFVDYSIGNWECKCGNLKGLHHLRTTCRNP
                     SCGARIRTDPFHAGDVLCHRCGTFNRNIVTFCNRCGDPVSLQLKYDVAECEERGMTYA
                     APLKVTIRLTVYDKDAETGNKSVRDIKEQEVFFGEIPLMTENGTFIINGTERVIVSQL
                     HRSPGVFFERVPAQGYYLGKIIPYRGSWVEFEYDVKNLLHVRIDRKRKFYGTVFLRAL
                     GFKSDEQILRTFYTIEKLKIKDSKLLWTVSPGLVGLKLSHAITAKNGDTVVPQGRKIT
                     ASVFRDINKNKIEQVEVAANDLEGAYVAADVIDMSTGEVLLESNHELTSSGISKMMEA
                     GIEEFEVFFPERDEVGNVISVTLKKDSVKTQSDALLEIYRKLRPGDPPTLDTATQLFH
                     GMFFDPRKYDFSRVGRMKFNIKLFDRADATPLDKRTLDPDDFTGTIRYLLKLRKGIGL
                     VDDIDHLGNRRVRAVGELLENQFRIGLVRMERAIKEKMSVYQEMSTAMPHDLVNAKPV
                     MAAIREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPTHYG
                     RICPIETPEGPNIGLISSLSCFARINEYGFIESPYRRVKGGVVVEEVRILNPGDSNHR
                     VGEVMTAAEAAKIKTDLASRQTLEWEAHCDYLSAWEEDKYTIAQANVELDQNGRMVNE
                     LVNAREAGDFVLTSRDEVEYMDVSPKQLVSVAASLIPFLENDDANRALMGSNMQRQAV
                     PLLRAEAPLIGTGMERVTARDSGAVVICKRSGVVDSVDSERIIVRVEGAAHEAQLSRE
                     VGADIYQLTKFKRSNQNTCINQKPIVFAGQRVKKGDVLADGPCTDSGELALGRNVLVA
                     FMPWRGYNFEDAIVVSEKLVKEDYYTSIHIEEFEIEARDTKLGPEEITRDIPNIAESF
                     LRNLDESGIIRIGATVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVKDASLY
                     CPPGIEGTVVDCKVFSRKGAELDERSKWILQSQIDRLYRNLEDEKRILNDERAKRLGN
                     LLEGKQLQADLHDEKTNRKLLSKGVDLTRDIVEKLRARDLKRIRLKDKDPRLNEQIDE
                     IEEMTSRQISVLEKITEERIAKLRKGDELPPGVIKLVRVYIAMKRKLSVGDKMAGRHG
                     NKGVIARIVPEEDMPHMPDGTPVEIVLNPLGVPSRMNVGQILETHLGWAAKALSRHFA
                     TPVFDGAAELDIKHQLEEAGLPASGKVQLVDGMSGLPFEQPVTVGYIYMLKLSHLVDD
                     KIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYVLQELLTAKSDDVY
                     GRAKIYEAIVKGEAAAEPGVPESFNVLIRELQSLCLDVELMKKPREVLDTALAAD"
     gene            28006..>29277
                     /locus_tag="VFQ79_09475"
     CDS             28006..>29277
                     /locus_tag="VFQ79_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU0139929.1"
                     /translation="MIADFDSIRISLASPEKIRSWSHGEVTKPETINYRTFKPERDGL
                     FCARIFGPVADWECLCGKYKRMKHRGVICDKCGVEVTLSRVRRERLGHIELASPCSHV
                     WFFKGLPSRIGYLLDITLRELERVLYFEAFVIVDPGEVPGISRGEVISDERKRQLDQE
                     QPGKFVAMMGAEGIKELLKKIDVESLSLEIREKMKTEASQQKKLKYAKRLRVVESFRK
                     SGNKPEWMILDVLPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKKLMELHA
                     PDVIVRNEKRMLQEAVDALFDNGRRGRVLRGANNRPLKSLSDTLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYHRLEQRGHCTTIKQAKELVEQ
                     QDPVVWDILEEVIRDHPILLNRAPTLHRLGIQ"
BASE COUNT         5838 a         8486 c         9089 g         5864 t
ORIGIN      
        1 gctggaacat ttggtaaata tgatgtgcct cgtggtgcac catgtactcg atctggaagt
       61 gtacgttgta gtggaggtat tcccggtgac ggccgggccg gtgccacgcg gcggcatcca
      121 gagcttccag tttgctcagc agcttttcgc gctcctccgc aaagccctcc ttcacccgcg
      181 ccggaccgat tgcgagaagc cgctcgaatt ccggatcctg atccggatca taaggttcaa
      241 cttgcgggtt atcggattcc agcatctgga tcacccggca ttcgaacacc cgctgcacac
      301 gccacaggtg gcagacgagc tcaatcaggg accattttcc ctctaccacg cggcaggaaa
      361 gggccgcttc gtccagaccg gaggagagcc gagcaatggt ggaggcctgt tcgcgcagtt
      421 tctctatcag cgtccgataa ggactttgta gatcagtctg gacgtttggg cacatgacag
      481 gattatacgg cggatttcgc gttatctcat acccgtataa tgtttacgcg aaggtcacgg
      541 agacaaaatg ctaaacacaa agatgttgct cgtacttggg ctggccgtta gctcgagtct
      601 ttttgccgcg cagaaaaagc gggtggccgt tctggatttt gattacgcta cggtttcgag
      661 ccatgtcgcc gccatcttcg gaacgaacca ggatgtcggg aaaggaatct ccgacattct
      721 ggtcgacagg cttgtgaaaa gcggcacgta cgacgtggta gagcgcaaag cgatcagcaa
      781 gattatcgcc gagcagaact tctcgaacag cgaccgcgcc gacgcaagca cagcggccaa
      841 attggggcgt gtgctcggtg tcgacgccat cattattggc agcatcacgc aattcgggcg
      901 cgacgataag acgaccaata ttggcgggca ggccgtcggc ggcaggctcg gaaggtacgg
      961 actgggaggc atcggccgca gacaagcgaa agccgtcgta ggaatcaacg cacgcatgat
     1021 cagcaccgac accgctctga ttcttgccac ggcggcgggc accggcgagt cgcagcggtc
     1081 cgggacggcg ttgctcggtt ccgggggcag tgccgccaat catgccggcg gggctgtcga
     1141 tatgtcgagc tctaatttcg ccgacacgat actgggtgag gccgtccacg ccgcagtcgg
     1201 gcagctcgcc acgcagttgg agcagcagac ttcccggttg ccccagaata cggtgaccgt
     1261 tcaaggtcta gtcgcggacg tcgcaggcga caccgtcgtt ctgaacgtag gaaccaaagc
     1321 tggcgtgaaa gtgggcgacc ggctccgggt cgagcggccc gtgcgggagg ttcgcgaccc
     1381 ctccaccggg aaagtgatcc gccggattac ggaccaggtc ggcgaggtcg ccgtaacgga
     1441 agccgacgag gtgtcatcgg tcggaaaatt caccggctcc gctccggcga aggtgggtga
     1501 cgtagtcagt tccgcacagt gaactcccat ggaaattggc cagcgaattg gcgattacga
     1561 gattgccggc atcctcggcg ccggcggaat gggcaaagtc tataaagtcc ggaatgtcat
     1621 ttccgaccgc aatgaagcca tgaaggtgct tctgcccagc ctgaaagccg atcccgaact
     1681 gggggatcgc tttctgcgcg agataagggt gcaggcaagc ctcgatcatc ccaacattgc
     1741 cgctctccac acggcgcaaa gcatcggcga tcaactggtg atgatcatgg agttcgtgga
     1801 gggcgtctca ctcgacaagc tgatccagcg aggccccgtc cccctcgaca aggcgctcaa
     1861 ctacacggcg caggttctct ccgccctggc ttatgcgcat gcccgcggag tcgttcaccg
     1921 ggatatcaaa ccgcagaaca tcatgctcgc cgaaggcggg gttgtgaagc tgatggactt
     1981 cggtatcgcg cggcttgccg cggatcggcg gctcacgcag accgggcgca cggtgggctc
     2041 tctctattac atgtcgccgg aacagatcaa aggggcgctc gatttggatg gccgcgccga
     2101 cctttattcg ttgggtgtca cgctgtatga gatggtgacg ggcaagaagc cgttcgtggg
     2161 cgacagcgac ttttccatca tggccgcgca cctggaaaaa gccccggttc cgccggtcca
     2221 ggtggacccg acactaccgc cggaattgaa cgacgtcatt ctgatgtcca tcgccaaaga
     2281 cccttcgcag cgattccaaa ccgccgaagc ttttggcaat gctctagcgg cagttcgcgg
     2341 ggccgtggcg gcgcttggag ccacacccac catcggcagc acgcaacagc agccccctgc
     2401 ggccgcgccg gctgcggcaa cacgaagcag gcgcgggttg tatatggcgg tgggctccgt
     2461 gttgacgctc gccgtcctca ttggtgcggc aacgcaactt cccaagttct tccgcaccag
     2521 cgctgacgga ccagcagcgg atgccattgt cgagcccgcg caaacgccac tgcggccgga
     2581 gcctgtcgcg caaacaccgg agatctccac tcagaccaac gcgcctcaga ccgccgtgga
     2641 gccggcgccg gccgtcgaac ctccagcggt aaagatgccg gacgcagcgc cgaagcgcgt
     2701 ggagcggcaa gccacaacag tcgccgttcc cgcacccagg gcgagttcgc cggtccccgc
     2761 ggccgtcaac acgccaaccc aatcagtttc accgacggct gtcgcaccaa ccgagccggc
     2821 tggggagcgt tccgttcaac ctgcccccga ccaggcggct ctccagaaac tcgagcagcg
     2881 catgatgctg atggccgcac gcgtcgccgg tgtctccgct accttgacac gaatggagag
     2941 ggagatggcg gcacagggat tggggatgag aagcgatttc gtctccgcgc agcggcgcct
     3001 ggaattgcac atggaccagg gcgaagccgc gctgaaaaaa ggcgacgtag ccgccggcgg
     3061 ccgaagcctg gacgcggcgg aacgcgaact cgaaacactc gaaaaagctc tcggccggta
     3121 aagccctatc agccttcgat ccaagcggtt ggcgggcctc atggtctacc gctccgttgc
     3181 catcgcggct cagctacacg ctccccatgc aagaatagga aggaatgatc gaaaatctgc
     3241 gtcccggtgc gtctgcgaaa cgggcacggg tagttctgtt tgatttcgat ggaacgctgt
     3301 cgctcatccg ctccgggtgg gtggaagtaa tggttcccat gatggtcgaa gttctagcgg
     3361 agctgaagag tggtgaatcg gaagccgaac tcaagcaggt ggtggacgag ttcgtagcca
     3421 ggctgacggg taagcagacc atctaccaga tgatcgaact ggagaaccag gtccgcgcac
     3481 ggggcgggga acccgaggat ccgctggtat ataagaagtg gtacctggat cgcctttggg
     3541 atcgcattcg cggccgtgtc gacgaactga aaaaagggca ttgcgcgccg gagaagtacc
     3601 tggttcccgg ctccctcgaa ctgctggaag ctttgcgagc aagagggctc aagctgtatc
     3661 tcgccagcgg caccgacgag agctacatga aacgcgaggc ggagctgctg ggtgtgacac
     3721 cgtacttcaa cggcgtctac ggcgcgctgg acgattacaa gagtttctcg aaggcgatcc
     3781 tcattcaacg cattatcggc tcggcggaat gcgccggtga cgagtttctc gggttcggcg
     3841 acggctatgt cgagatcgag aatatcaagc aggtgggcgg cgtggcggtg ggcgttgcga
     3901 ccgacgagcc ggaatgcgaa cgcatcgacg agtggaaacg gaatcgcctg gccggtgtcg
     3961 gcgcggattg gattgtgccg aactatcgct cgagcgaccg tttgatggag gctctttttc
     4021 agtagcacgc caatgccctc ccgctatccg ctgtttgacc ggtcgcggct cctcatcaag
     4081 ccgctttcag aacgtatcca cgatttacat gtcgagcact ggctcgggct cgccgatccg
     4141 gctcctccat tttcccatcc ggccctgccg gacgtggccg cgcggacggt gtgcgcgaga
     4201 gagaaaggtg ccgcgcgcat cctcatgatg ggtgcgcacg tgttaagggc gggggtgagc
     4261 cgccatctca tcgatctgat ggagcgggga ttcatcgatc acatcgcgat gaatggcgcc
     4321 ggagccatac acgattacga actggcacgc atcggtggga ccacggagag cgttgcccgt
     4381 tatattcgaa ccggtgaatt cggattgtgg tgggagaccg gcgaattgaa cgaatggatc
     4441 accggcgcag cctccgccgc cgaggggctt ggagaacaca tcggcaaaag gctggaggcg
     4501 agttcgttcc gctatcgcga cgttagcgta ctggccgcag cctggcgcct gggtgttccc
     4561 gttacgatcc acgttggaat cggctacgac atattgcacg aacacccgaa ctgtgacggc
     4621 gcggcgctcg gtgccgccag ctatacggat tttctgatct ttgcgcgctc ggtagagcgg
     4681 ctcgaaggcg gagttctgtt gaatttcggc tccgccatca tgggaccgga agtgtacctc
     4741 aaggcgctct cgatggcgcg caatgtggcg ggccaggaaa agcgcgtaat tcgcgatttc
     4801 acgacggcgg tgttcgacct cgtgccgatt gaaggcgaca tccaccacga attgacgaag
     4861 cgcgacccgg gctacttctt ccggccgcat aaaaccattc tggtaagaac ggtcgcggac
     4921 gggggaacca gtttttattt ttgcggcgat caccgcgcca ccgtaccggc ccttcgaaac
     4981 gccttgctgg aggcggcatg acggcggaac ggattctggc cgccttcccc cggctttcgg
     5041 cgctggtggt gggcgacatc tgcctcgacc gttggtgtac gtacgatccc gctgaatcgg
     5101 atccctcgcg cgaaaccggg attccgagaa taggtgtcgt ctcgacccag atcacgcccg
     5161 gagccggcgg taccgtggcc aacaatcttg cggcgctggg cgcgggacgc gtagccgtgc
     5221 tgggtgtgat cggcgatgac ggttttggat ttgaattaag ccgtgcgctc cgttcgagag
     5281 gaattgagcc ggacctgctg atcaccacgg cggacgttca gaccttcacg tatacgaaaa
     5341 tcatcaacgg gatgagcggc gtggaggacc gtcccaggtt ggatttcatc aacacgcggc
     5401 cgctcgcgcc cgatgtggaa cgcgtggtga tcgagcggct gaacgcgagt gcgggtgagt
     5461 tcgacgtcat cttcgtttcc gaccaggcgg agaccaacgc agggggcgta gtcacggaag
     5521 gtgtgcgcca gtggcttgcg caatggagga ccgcttatcc cgacaagatt gtttgggtgg
     5581 attcgcggat gcgctccgac cggttccgca aagcgatcgt gaagccgaac cttcaggaag
     5641 ccgaagagtg ctgccgccgt ctgttctcgc gggtcgattt tgaagcgctg cggagacaca
     5701 ttgactccga tctgctcttc gtcacgcacg gcgagcgcgg tgtcctggtt gtggagcccg
     5761 gaagcgagtc ctgggtggaa gcgcgtccgg tggaaaatcc agtggacatc tgcggggcgg
     5821 gggacagctt ctcggcggga gccgggctgg ctcttgctgc cacgcgtttg ccggttgccg
     5881 cggcgcggat cggcaacctg gtggcctccg taaccattat gaaaaagggt accgggaccg
     5941 cgtcgccgga cgaagtgctc gcagccgaga agcgtttgca ggtatgagaa ttctcgccgt
     6001 agacattggc ggcacgaagt tttccatggc ggcgttcgaa aacggacgaa tcaccagccg
     6061 gttcactcta ccgaccgatc gcgatggcgg accacagtgg atggtgcagc agattgccag
     6121 aaccggccag gagtggcagc gtgatggacg cttcgaacgc atcggtatcg ggttcggcgg
     6181 acccgtgctg ttcgacaggc agcagatagc gctctcgacg cacgttggcg gctggagcgg
     6241 cttccccttg ccggaagttc tggaacggga gttcggaaca ccggcggtga tggataacga
     6301 tgccaatgtc ggtgcgctcg gggagtggcg ttacggagcg ggcgccggct gcgacccctt
     6361 gttctacatg accctttcga ccggcatcgg cgggggcatc ctggtgggtg ggcggctgtt
     6421 gcgcggcgcc gatggctacg cgggcgagat cgggcatctc accatacgcc cgggtggtcc
     6481 ggaatgcctg tgcggcgcac gcgggtgttt cgaacgcatg tgttgcgggc tgtggctgga
     6541 acgggatcat ggacggccgc cggatgaatt gttgcgcgat accggcttcg tgagggagta
     6601 cgtcgtcgat ctcgcccttg ggctgaaggc ctgcatcatg ctgttgaatc ccgcgagaat
     6661 agtgataggc ggcggcatca gtaaagcggg cgaagcgctc tttagacctc ttcgggcgga
     6721 gctcggccgg cagattaccg cctggtcccg ggcccgtgtt gaggtggtcc ccgccgcgct
     6781 cggtgacgac agcgtgctct atggtgcgtt gtgcctggcg cagaattagc gcccgaacgg
     6841 gaaatggaat ggttatgaat tcagccgagc tttataagaa cgagctcatg gaaacggtcg
     6901 gcaagatcga cacaggtaaa gtgcagacgg cgatcgaatg gttcgaggag gcgcgtgaca
     6961 aggggcggca cattttcgtg tgcggcaacg gcggaagcgc atcaacggcg tctcatttcg
     7021 cctgcgacat cgtcaagggc gccagttaca atcgcgataa gcggtttcgt attctggcgc
     7081 tgaccgattc gctacccacg ctgacggcct attcaaacga cgtcagctac gactgtgttt
     7141 tcgcggaaca gttgaaaaac tttgcggagc ccggagacct ggtgatggcg atcagcggtt
     7201 ccggcaactc gccgaacgtc gtctgcgcgg tggaatacgc caattcgatc ggatgtaaaa
     7261 ccatcgccct gaccgggcgc gacgggggcc ggctgggcgc cctggccgga ttgaacatcc
     7321 aggtccctgt ccagcatatg ggccgaatcg aagatgcaca cctgattgtc tgccacatga
     7381 tcggctatca tttcatggac tacagcgggt agcctgacgg agccgcgcgc gcctatggta
     7441 ctggtattac cctaccggta ctgcaaaggg atagtttcgg taccttcctc tggtattatt
     7501 tcctcgttca ttgtctggga gtgatgtctg taagggggac acacatgagt actcaaggat
     7561 ttatggcgcg gggcccagct ccttcattgg cgggagtgtt tgtgtgggaa ctacccgatc
     7621 actccgtgac ggttcagttg ccggcgcgag tcatctcgga acttgccggc agccccgaag
     7681 gtgctgatct gcgccagtcg gcgggactgc aggacctggg cggcgccatc ctgggcagca
     7741 tcgaggaaac ggcgggtgtt gcactcctgg tggaagattg tctctccctg cctttcttca
     7801 ctggatcgga aggccgtttt gcggaagatc gcaataaaga ccgctttcgc gatgtcctgg
     7861 gaacgtgggc gcccgggccg gaccggaggg tcttcgctct tggcatctac cggcgggttc
     7921 tcggccaact ggtgccttcg gcggaggatg tggcgcttta ctcgtctcag ttcagcggcg
     7981 ctccggcgat cctgctgctg atctcggccc ggaatagcca tccgcgcgag gcatcgctgt
     8041 tcgtatggcg tgccaacgcc ttcgtggtag ccggttcgcg atttccactc gatggaacgg
     8101 ctttgctggc tttttccgga gcgaaacaca cgccgccttc gccgctggcg gtagcgccgc
     8161 ccggaccgca ggcggtccgg aataacggag tggcggaggt ccaacagcct gtggccgccg
     8221 tgcatcctgc gctagcggct tcagtagttg cgcgtcccgt atcggcgccg ggcgtgtctg
     8281 tcaggcgctg gatcctgcct gtcgttctgg gcttacttct gttgatggtc ctcacgttgg
     8341 gcggactggt ggcccggctc tattggccgg caaatgtgga gacagtacag agcggaggcc
     8401 agctcgacct tcgcgcgatg cgctatccgg acagcatttt tctgacgtgg aaccggactg
     8461 caccggtcct gagttcggcc accggcggtc ggctggccat caccgacggg gaataccata
     8521 gagagctaca gctcgaaaaa caggaattac tttcgggcag cgtctcatat gtgccggcgg
     8581 gaaacgacct ccattttcgc ctcgatgtct tcatgtcgcc ggatcggacc ataagtgaga
     8641 gtgttcgcgt aattggcggg ctgttttcag aactgcgcag gatactaccg cccatcggta
     8701 ctccgccacc gagacaggag gtgtcgcgcc cgcctgggtc cgccacggtt tcaacacagg
     8761 agccgagaca atttgaagga ccgccatcgc tcgcttccag tccgcgcgaa gtttcccggg
     8821 gctcttccga tcgcgacgcc tcaaaccgta cggtctccat gccgcaaccg ccggcgctac
     8881 cgcagcggat ggtgcctgac ctcaccacca tcagcgctgt tcagaggccc tcgattctac
     8941 ccgatgcgcc gcctccgccg gagcgaagtg tccccggcgc tcagcagagc gtgctgtctt
     9001 cacagcccct tggcacgtcc ggatccgccc agccggctaa accagttgtg tcaccgccgg
     9061 ttccgccggt ggcgactcgc aaagtcatgc cttcgattcc cggaagtgtt cgctatctgg
     9121 cggaaaatgc cggcgaagtt gaagtaagag tacatgtgga tgctcgcggc aacgtgacca
     9181 acgcggccct cgtccgcgct gaaaagagcg ccggaatctt cctctcgcgc tatgcgctgg
     9241 cggctgccta tcaatgggag ttcagaccgg ccatggcggg cggcaaaccc gtgaacggcg
     9301 aggtgctgat caggttccaa ttcgcgtccc gttgacgtgt tcacccgagg gaaataatca
     9361 atttaccgac gccgtcgtcg tatcgttcga gcagggagaa ggctttctcg atgtcttcga
     9421 tggggcgagt gtgcgtcagc ataggcgcga acagggcacg gtggtttttt atcagatcca
     9481 aggcctggtc cgattcatga ttggagcgcc gcacgcttag cagagtccct tctttacggc
     9541 gcagcgtatg gaaatcgaat tctacgcgga cttcggatgg aatacccgta accacgacgc
     9601 gtccggctct gcgaatggcg ccgatggcct gatcgagtga accgcctttt gccgcacagt
     9661 cgatagcgaa atccactcct cgttgtcccg tgtcggctaa gatctggcgg actgtatcta
     9721 cggcggaggg gtcgatggtc gcatccgctc ccatccttat cgcaagctcg cgccggtgag
     9781 ccacgggttc cacagcccat acacggacac cggacaattt cagaatcgca atggtgagca
     9841 gcccgatggg tcctgcgccg aagacggccg ccgtatcccc caaccgcggt tcggccaact
     9901 tcatggagtg cagagtcacg gcgagcggtt cgaagagagt ggcttcgccc aagtcaaggt
     9961 tcggcgccaa ggggaggagg ttggccacgg gcaaattcac caggtcccga aagaagccgg
    10021 ggtctccggg ctggctcaaa aagcggatgt ggttgcagac attgtgccgg ccggaccggc
    10081 agtactcgca atggtaacag tagatggcgg gttcgagtgc ggcccggtct cccggactcc
    10141 atccgcttac cccggggccg gtgccgacta ccactcccgc cggttcgtgc cctaacacca
    10201 ttggaaacag ggcgggagca tcccccaccg agccttccga gaaattgtgc agatcggacc
    10261 cgcagacgcc gaccattttc acccgcacct gtacttctcc agggccggga ttctcgatct
    10321 gcccgctcac caggttgaac cggtgaggtg caaccagttg cgccactctc atggaattat
    10381 ttcttgaaca gactgtcgaa cgccgcccgg gcatcgtccg cgttgcgggg agccggcggt
    10441 ccgctgggtg caacgcccac cgatgcggag ggcgcgacat cgcgggccac cgtcacacga
    10501 ggcttgaaaa gctcgcactc attccgctgg tctttttgcg cgatgcggat gggaatgggt
    10561 ttcaggcact gaaatcttgt agaaggctca aagtgtgcac actgcttgca gcagtgcagg
    10621 gccatgttgc acttcgggca taatcctttg aagtccgtat ccgcgggaag cgccgtcgag
    10681 cagttgaaac agcggtaggc cgtgacggac tgcactaatc tggggaggcg aggtcccgtg
    10741 acgtcgatag ggggacgagg cccctggttc ctggggcgct cttcccggga gggaccccca
    10801 cggtcgcgat cggaatccat gtagccacgt tgtttgtact ttcgatcgtt atccatggct
    10861 agctattact cctttggaac ccttgaagca gcagggttat agtacgtgcg agcaaaaaag
    10921 cgatggcttg caggcatcca gctcggcagt gcgcatcggg gactggaaag catcccgatc
    10981 gctccgtcag ggtgggtgag agggacgctg cggaagttac cttggggcta tgaacccgat
    11041 ccggtaaggc tcaaatacct atttatcacg gatctgttca cgacaccagc aaattctatt
    11101 gtacatcatg gtcacaaaac gaaccgcggt ttttctattc cactgcccac ttgatcagag
    11161 agttgcaggg gcgggtcagg gggtcctcgt agtggttggg agagatccgg aacgaatagc
    11221 cgagccggcc ggtgtgcgtg ggaatgtaat cctttgcaaa cacaaaagcc gaaccctgtt
    11281 gttccacagc cggtagagct aaaacctccg tttcttcgag ctgcccgctt ccgttgacac
    11341 ggcccacgac agcctcgacg cggatgtcat ccggcttcag tcccgccaga tcgatggtcg
    11401 tgcgaaacgg aagcggacgt ccgctgacaa caaccccttc cggttccgga cccatcgcaa
    11461 taaaccggat gtgatcccaa gcccggacca cctccgcatt ccattgcacc cgctccctca
    11521 ctttgtcgaa tccaccctgc tgcaactcca ggaaggctcg ctgcgccgga gtgtagagct
    11581 ggctggcata ttcttccaac atgcgctgaa agctgtattg cggactgaca ttcctcaaag
    11641 acagcttgac ccgccgcatc cactctgctg gaacgccttc ctcccggtcg cggtaataca
    11701 tcggtatgat ttcgttctcc aggagcgaat agatggcgct ggcgtggatc tcgtcctggt
    11761 cttcgctgta atcctcacgg tcgccgatgg cccaaccgcc ggaagtttca taagcctcgt
    11821 cataccaccc gtcgagcaca ctgaggttga gaatgccgtt gatgccggcc ttcattccgc
    11881 tggtaccgca agcctcctcg ccgcgcctgg gattgttcag ccacaggtcg acaccctgta
    11941 ccagctcgcg agccacctgg attccgtagt cctcgataaa gaccagacgc ttataaagct
    12001 cggggtctct tgaaagctgg acaatctcgc gaatcaacgc cttaccagga tgatccttcg
    12061 ggtgtgcttt tcccgcgatc acaatctgga cgggcatgtc cgggttcgtg aggatctttt
    12121 tcagcctctt cgtatcacgc agcagaagcg tcgcgcgctt atacgttgca aaccggcgcg
    12181 cgaatccgat ggtgaaagca tcgggatcga ggacctccgc ctgcctgcgt aactccggtg
    12241 cggaggcttt tcgcgccacc gccgacgctg tcacacggtc ccgcacaaag gcgatgagct
    12301 gacgcttgcg gcggcggtgg gcctcccata gttcctggtt tgggatgtcc tgtacaaggt
    12361 cccatgtctg cggctcgtga aaacgctcat gccagtcggg ctgcagatac tggtcgtaga
    12421 tcatggccag gtcggtgttc agccaactgg gcagatgaac gccgttggta atggaggtaa
    12481 tagggacctc ccacgtcggc agttccggcc aaaggtcctg ccacatctcc tgggagacct
    12541 cgcggtgcag gcggctcacg gcatttcgat atgcggaggt cttgatggcg gcgatcgcca
    12601 tggagaaacg ctcctgcgga tcgaatccgt tgcggccgcc cagagctaaa aatccttcaa
    12661 acggaatgcc gccttcatta cagtacccat ggaagtagtc gtacatcaga cccggctcaa
    12721 acaggtcgat accggccggc acggatgtgt gagtcgtgaa aatattgttg ttgcgcgacg
    12781 ccgtcagcgc ctcttcgaac gtgaggttgt gctctttgat gagaacgcga atacgctcga
    12841 tggcgagaaa agccgagtgg ccctcgttca tatgaaacgc ggcgggctcc aggccaagcg
    12901 ccttcagagc gcgcagaccg cctatcccaa gaacaatctc ctggcggatg cgcgtgtgaa
    12961 tatcgccgcc ataaagctgg tcggtgatgt cgcggtcgtc gggcctttcg ttttccggga
    13021 tattggtatc gagcagatac agttttaccc ggccgacatt gatctgccat accttcacgt
    13081 acaccggtcc gtgaggcagg ccgacctgga ccaacagatc ttctccctta tcgttgatga
    13141 ccggggaaac gggaagggta tagaagtcgt tggtcgggta acgctcctgc tgccaaccgt
    13201 cgggattcag aacctgctgg agatatcctt tctggtacaa cagcccgata cccaccaggt
    13261 ggataccggc atcgctggcc gctttcagat gatcccccga caaaacacca aggcccccgg
    13321 aatagatggg gagacactcc gccaatccat actccatgga aaaataagcg accaggcgat
    13381 cccgtgcggg cgcctcctga tccatgtagg agtcgtagcg ctcgcaggcg cggcggtaca
    13441 aagcgaggta gcgcggatcc ttgcccgctt tctccaggac ggcctgcggc aggcgtccaa
    13501 gcatgagtac gggattgtgg ccacagccgg tccagagagc ggggtcgagg cgtcgaaaca
    13561 cctgccggat ggtgagatcc cagttccaca tcaggttatg agccagttgg gacagccgcg
    13621 agagcgtatc cggcagagcg ggacggacca ggaactcctt gaatcgatgg atatgaaaag
    13681 gcatgaagat ttaagtctat ccctaaaata ccagaggccc ctcggccagg cttctcagcc
    13741 gtcagcagca cggtaccaat gaccacggac gtgcctaaaa tcgtacgcca acgccggcct
    13801 tggctccaat tgtgggcagg atgtaatgat gaacggagaa aagctcggcg cggatgagca
    13861 ggcgtgcgcc taatgccgcc acgccgccgc cgcgccagtg caccgaccaa aaggtttcgc
    13921 tctgatcata caggaaaaag ggagtaacgg cgcgggaccg ctggaaaaca gcgccggcgc
    13981 cgcctcctac aaaaccgtaa gcgcgttcgg tgccgaaccg gtagagcaga ctgggactta
    14041 tcaaagtgcg ccgctctgag tattcagacc gctccgccac tcttcccgta tcgtctcttg
    14101 ccgtgacgat gtttacatcg atggccagct ttggcagcac cggcagcatc acggaacccc
    14161 catagacggc accgctccca ccccaccctt catcttgtcc gaacttgacg attccggcgc
    14221 tgccaaacac ttcaatggag cggggaggtt cggcataaag accggacatc aggctactca
    14281 gaaccatcac acacaggaag aacctgccca tattattggc catcgccgca tctcacggac
    14341 ctattttgct ctgaactgtt gaaaatatcc atatcttccg acggaaaagt tacaaggaaa
    14401 ctcaaacgta caataaaagc ttgtagattt tcgtatctga tacaccgaaa acggcctaaa
    14461 atggaggcat gaaactaagc gctcaggagg aatacggtct ccggtgcctg ctcttcatgg
    14521 cgcgtcatgg cgacggccat agcctcagta ttcctgagat cagccgtgcg gaaggactgt
    14581 ctgtgccgaa tgtggccaag ctcatgcggc tgcttcgcct tggaggattt gtcgcgagcg
    14641 cccgggggca atccggtggg tacactctgt cgcgtccagc gagccagatc acggttgcgg
    14701 aagtggtcga actccttggc ggacgcgtat tcagccggaa cttctgtcaa aggcatgccg
    14761 gcattgaacc tgtatgcacc catatcgagg actgctcgat ccggtgcctg tggagcactc
    14821 tgcaaacgat ggttgaaggt gtactcgcca aaaccactct tctcgacctg gtagtgagcg
    14881 aggccgatat ggccgcattt ctcggctcga atggttcggc gaaaacccaa tcgccggaac
    14941 tggtaccgga gcacacgatc gccggacagt gagagcgaag aattagcgtt ttccatcgaa
    15001 ctgtcattga gacgccgcag cctccgcgga gaggggagtt gtgtgcgggg tgttccatct
    15061 ttgatcccac cgtcctgtgc ttgctaatat cgaacagact ggtagtcact tgacagactg
    15121 gcggtaacgg gctggcgacc cgcattcact tacatgacca gcaaacactt ggaaccttcc
    15181 gcgcatttca atgttcttac ggagtcccgc atacaaaccg atggactttt ttcgctgcta
    15241 gaccccgaag ctttgtacga acgcccaatc ccggagcggc atcggatcat tttttacctg
    15301 ggccatctgg aggcgttcga ctggaatctg atcggacgaa ccggcctcgg tctcgaatcg
    15361 ttccacccgc aatacgatca tctgtttgca ttcggcatcg atcctcccga aggtcaactt
    15421 ccgaacgatc agccaccgga ttggccgtcg atagaagagg tgttggatta taaccggcag
    15481 gtacggcgca aattggatga agcctgggac agcgcatccc ctgagttgcg gcacgtcgcg
    15541 atcgaacacc ggtggatgca tgcggagacg ctggtataca tttttcataa cctggagtac
    15601 agccggaaaa agcaagggcc atccagggag cctgccgcat cgcccatact cgaaaattcc
    15661 ctgatcgata tcccggccgg ctttgccaca ttgggcagaa agaaggagga aggcttcggg
    15721 tgggataacg agtttgaaga acataaggtg catgttccgg cttttcgaat ccgccggcac
    15781 aaggtcgcca atggcgagta tcttcaattc gtgaacgccg gtggcgaagc accgttttat
    15841 tggacgagac gcggcggcga atggcactat cgcggcatgt tcaatgaaat tccgctgccg
    15901 cacgagtggc cggtgtacgt cacgttccaa caggcagtgg agtactcgcg ctggaaaggt
    15961 gtttcgctcc cgacggaagc gcaatggcac cgcgcggcat tcggaaccgc ggccggcgag
    16021 gaacggctct atccatgggg ggatgcgccg cccgacgcgt cccgcggcaa tttcgatttt
    16081 gatcgttggg acccggtgcc ggtggacgcc aatccttgcg gtgagagcgc cttcggagtt
    16141 caacagaccg tgggaaacgg atgggagtgg acctcgacgg ttttcggacc gtttcccggt
    16201 ttcagtccgc gttcctacta tcccggatat tcggcgaact tcttcgacgg ccagcactac
    16261 gtgctgaaag gcggaagccc gcgcaccgcg gcggcgctgc ttcgcagatc gttccggaac
    16321 tggttccggc cgcgttatcc gtatatctac gctacgttcc gatgcgttga ggagtaatag
    16381 atgctcgcgc gagctttccc cgcaaacgat cccctgctgg aatttgcaaa cgacgttacg
    16441 ctgggcctca cgcaggaaaa gcagagggag ctgccgtcca agtatctcta cgacgagctg
    16501 gggtcggttt tgtttgaagc catcaccttg ctgcccgaat atggtctaac ccgcgccgac
    16561 gaacgtgtca tacggcggag cgccccggag atcggccgct atttcggcgg cgccctgatt
    16621 gcggagttgg gcagcgggag cggaaagaag acccgccaca ttctgaacgc ctgcctgaaa
    16681 ggccccagcc tgacgtatgc tcccatcgat ctgtcgcccg ccgcgctcgc ggcctgcgcc
    16741 ggccagttgg gcaacggtgc cattgaagta cacccgattg ccacaagcta cctggatggc
    16801 attggccgcg ccgccgctct gcgtcagaga aaacaaaaac tgctggttct gtttttaggt
    16861 agtaccatcg gcaacttcga ccgcaatggc gcggtcgagt tcctgcgcaa aatcggcggc
    16921 tgcctgttgc ccggcgatgg cttgttgctc ggtaccgatc ttgaaaagcc gatcccggaa
    16981 atgctggttg cgtatgacga tccaacgggt gtgacggcgg ctttcaacct gaacctgctt
    17041 ggcaggatca accgcgaact gggcggcgat ttcgatttgc ggatgttcca gcatgaggcc
    17101 agatacgatg tggagcaccg ccggattgag atgcacctca gatccagggc cgatcagaca
    17161 gtaacgattc ccggggctca actgcgggtt tcgttcgttt gcggcgagac gatctggacc
    17221 gaagcatcgc acaaattcgc gctggctgaa ctgccgcgaa tggcggcgga agccgggttt
    17281 gtccaccagc aacactggat cgacgaagag tggcccttcg ccgaaagtct ttggatgcgc
    17341 gccgccggtc catagaatgc ggattcccgt cgagtttcgc caggtctctt ttgaaatagg
    17401 gggcaagacg gtcctgcggg atttttcgct ggctctcagc gaaggggaaa ccgtcgcgct
    17461 gctcggccgc agcggatccg gaaaaacgac tgctctcaag ctggtcaacg cgctgctgat
    17521 gccggcttcc ggcgaggtgc tggtagaggg gcgtgcaacc acggcgtggg atcccatccg
    17581 tctgcgccgg cgaatcggct acgtcattca ggagaccggg ttgtttccac acttcaccgt
    17641 cgccggaaac gtcgggcttg ttccgcgcat ggaaggctgg ccggaggagc ttataaacaa
    17701 acgtgtggac gagttgctcg agatcgtggg ccttgcgccc cgggagttcg gaccgcgtta
    17761 tccccgtgag ctttcaggcg gacagcggca gcgcgtcggc gtggcgcgcg cgctggccgc
    17821 ggacccgcct ctgctgctgt tcgacgagcc tttcggcgcg ctcgatccgg tcacccgcgc
    17881 cgaggtgcag gcccattttc ttcatctccg aaactcgttg cggaagacgg ccctcttcgt
    17941 gacgcatgat gttcgcgagg cgcttcatat aggaaaccgg attgttctgc tcaaagacgg
    18001 atccatcgat ctgataacaa cacccgccgg attccaaacg gccggtactc ccgaggcgca
    18061 ggcctttctg gcttgtctca gttgactgaa cttatgcgat ccgggcttgc gggcgacatc
    18121 gttcatctga cgctcgaaca tctgatgctt gtcgtgacgg ccgtcacggt tgccgttgcg
    18181 attggcgtgc cggcgggtgt gctgctcgtc cggcgcgcga agtggcggcg atggctgctc
    18241 ggcttcacga atatcgtgca aacgattccg agcctcgcgt tgttcggctt tctgataccg
    18301 attcccgcca tcggtggtat cgggcggcgc accgccattg tggcgctcat cctttatgcg
    18361 ctgttgccgg tgattcgcaa tacgctggtg ggtattctcg gcatcgaccc tgctgtcagg
    18421 gagtcggccg tggcgatggg aatgacgccg cgtcaactgt tatggaaagt ggagatgccg
    18481 ctcgccgcgc gtaccattct ggcgggagtg cgtgtcgcca ctgtgactac aatcggcact
    18541 gcaactattg gagccgctat cggcgccggc ggcctgggcg tgttcatttt ccgcggcatc
    18601 gcatccgtcg atacgactct gattcttgcc ggcgctctcc ccgccgcggc ccttgcactg
    18661 ctgtttgatg gaggtttggg ttggatcgaa aaacggttgg ctgttgtgta atttcgctgt
    18721 tgatcctggc cgcctgcggt ggaggcaatc gcatcgtggt cgggtcgaag aatttcaccg
    18781 agcaggtgat tctgggggaa atcgtcgcac aacatctgga gcgccgcttg ggagagcgta
    18841 ccgaaagaag actgaacctt ggtggaacac tgcttgcgca ccaggcgttg ctgtcggggc
    18901 aaatcgacgt ctatcccgaa tattccggca cggcactcac cgcgatattg aaccgcgatg
    18961 cagccgccga tgcgggtgcg gtctatcaaa tcgtggggac agcctatcgc aagctgggtc
    19021 ttgaatggct ggcaccgctc ggcttcaaca actcctttgc gatggtcatt cccggagagc
    19081 tgtcaagacg ccagaatctg agcacgctct ccgaagcggt gcggtggcag catgcctgga
    19141 ggctgggtgt cggctacgag tttgaaggac gtccggatgg cttgccggcg ttactgcgca
    19201 cttataagct ccaggtttcc ggccagcccc gcagcatgga tttaggcttg ttgtaccagg
    19261 ctctggaacg ggggcaagtg gatatgatcg cggcgaatag caccgacgga ctcctctcga
    19321 agatggacac cgctgtcctt gccgatgacc gcggtgcctt tccaccctac gaggccggat
    19381 tggtggtgcg gtcggagacc ttggccagag ttccgaaagt gcgtgaagcg ctcgccgagc
    19441 tgtcgggaaa aatttcgacc ggcgtgatgc agcgattgaa ctaccgcgtc gacgtggacc
    19501 atgaagcagt gggagccgtg gccgcggatt tcctgcgcga agccggcctg tagcggacgc
    19561 cactcgcgcc agtatccccg cggacgcgag tatacaatgg tgcttcaacg aggaacgcca
    19621 tgcacaaatt tttcctgctg cttcttctcg cggccagcat gccggccgcg gatctcaaag
    19681 tcggcgtcgc acggatcaag atcacaccgg acgggcccat ctggatgtcc ggctatgcca
    19741 gccgcgacaa accgtcccaa ggcgtggtcc aggaactttg ggccaaggcc cttgccttcg
    19801 acgatggccg cgggcgcgtc gtaatcgtga caaccgatct gatcggtctg ccccggtccg
    19861 tttccgacgc tgttgcggca caggtgcaga aacagcacgg gctggagcgc gggcggttgt
    19921 tgctgaactc ttctcacacc cacaccggac cggtcgtccg gccgaacctg aacgtgatgt
    19981 atgacctcga cgacgaaggg gacaggaagc tgaaggagta ctcgcaacag ttgacggatc
    20041 gtctgataag cgtcgtcgga gcggcgctcg gcgatcttgc cccggcccgc gtcgccttgg
    20101 gacatggaaa tgccggtttt gccatcaacc gccgcgaacc cacggcaacc ggcgtcaaga
    20161 ttggagtgaa tccggcggga cccgtcgatc atgacgtccc cgtcctgaag ataacgtctc
    20221 cggacggaaa gacccgcgcg attctctacg ggtatgcctg ccacaacacg acgctgggcg
    20281 gcgactttta ccagatcacg ggcgacttcg ccggctactc gcaggcggag atcgaaaaag
    20341 cccatcccgg cgcgacggcc ctgtttcttc aactctgcgc cggcgatcaa aacccgaatc
    20401 cgcggggaac gctcgatctg gccgagcagc atggcaaagc gttggcttcc gaggtgtctc
    20461 gggtgctcca ggggtcgatg aagcaggtcc gtccgccgct ccgctttgcg tatcgtgcca
    20521 cggagcttga gttcgctccg catacccgcc agacctttga agagcagctc aaaagctcca
    20581 accggttcgc agtaaggcgg gccaagttga tgttgcgcgc ttacgacgag ggccgcccgg
    20641 ttcgccgcgt tccatatccc gtgcaggcgg tccgcttcgg gaaagatctt acgctggtcg
    20701 ctctcggagg cgaggtggtg gtcgactacg ctttgcgaac gaagaaagag tacggcacca
    20761 gcgaggggct ggtggtcgcg gcgtactcaa acgatgtgat gtgctatatc ccgagcctgc
    20821 gcgtactcaa ggagggcggc tatgaggcgg tcgacagcat gatctattac ggccagccgg
    20881 gtcctttcaa cgaacaggtg gaggagatgg tgttcgacgc cattcacgac gtgatgaaac
    20941 gcgtggggcg aaggccggag aagcggtagt tggcagcggc cgctaccgcc ctgctgggtt
    21001 ctgctttcac gatcgccgcg tgtttcggtg cggggttcgc tctctttccg aagctgccgc
    21061 tggcccggtc tattcgtttc gcgctgggcg cagcgctgtt gagcacggct gttttccttc
    21121 tcctgttggc cggcgcaggg ggacgaacgc cgtttgcggt gttgggtgcc ggggccatcg
    21181 cggcaggggc gtttctcaaa ggccgtattt cgaaacggga atcggcgccg tggccgctcg
    21241 cggcgaaagc gggcgccgcc tttctactgg gcgcctatgg ggcgctctac ctggttcacg
    21301 caatggcgcc cgagaccgct ccggacgcca ttacttacca tctgggactc gtagccgaag
    21361 cgttgcgcct gggccggttt cccaaccgga tcggtttttt tgaaatgctg ccgcagggcg
    21421 ccgggatgct gtttgcccac gcctttgctt tcggtgccca ttccgcggca aaactggtgc
    21481 actttgcttt tctggtacta acctttccgc tccttctgag agtaggagga aagctcggct
    21541 tgcattggag tgtctccgcg gcggcagccc tcgtttatgt cctgacgccc gtggttgccg
    21601 tcagcggcac gtccgcctat aacgacgccg cgctcgtgtt ttgtgtactc gccgtctttt
    21661 atctgctgct gtgctggcgc gaggcaaggg actggcggtt gctggtgtgc gccgggttaa
    21721 tagcgggctt ctgctatgcc attaagccca ccggcgcggt tgtgcccgtc gccggttttg
    21781 tgtggatagc aatcgcggca aggtcgtggc gatcgaatct tgtctttgct gccgcggcgg
    21841 tcctgatgtg tgcgccgtgg gtggtgcgga gcgccgttct caccgggaat cctatcgcgc
    21901 ctctgggaaa ccggctcttt ccgaatcctc actttcacat cacgacggaa gaccgcctct
    21961 ccgcaacgct tagagagtat ggaggcgtca acgccgcggc cataccccag gagatcacca
    22021 ttcgcggcgg caaactgcag gggctgctcg gacccattta cctgctggcg cctctcgcat
    22081 ttctggcgat gcggcggagg aacgggctca cgctggccgc ggcgttgctg gtgatggccg
    22141 caccgtggct gctcaacaca ggtacccgtt ttctaatgcc ggcgctgcca tttctgctgc
    22201 tgttgctgtt tatggctctg ccgcgccggg cgttggttcc ggtggtgctt gtccatgcgt
    22261 tgctctcctg gccggccgcg gtgagtgctt attccgatcc ctccgcctgg atcttgcgag
    22321 ggtttccact gaaagccgcg tttcgaatca cgcccgaacg ggaatacctg cgggagcgag
    22381 tgggcggctt cgctcttgcg gaggctttgc acggaatgac ggagcctggc gagagggtgc
    22441 tggatttgtt tggcattccg tgggcgtact cgcattttgt gccggtaggc ccctggcatt
    22501 cggcgaccgg cgaccggctt tccaccgccc tccagttggc tgcgaaccct gccgcgctgg
    22561 acgtcatcgt ggcggaatgg cccgaacagc gcatttcggg cgtgcgtttc cgtgttggac
    22621 aacccttcga tacggtctgg gccattcacg agattcaact gcgcaacggc agccgcatcc
    22681 tccggccggt gcgttcgtgg ttcttctcgg ccaatccgaa cccgtgggag atcctgttcg
    22741 ccttcgatcg caatgtggcg tcggcgtggc agtcctggaa gcacatgcgg cccggcatgt
    22801 tcatcgagat tgaactggcc gaaccgcaac gggcgaccgg ggccacaatg ctggccgttc
    22861 gttcggatgc aaaccgggca atagaagtgg acttgcttac gcccgatgcc gcgtggcagc
    22921 cggtgccgca ccgtttggag acccggccgc cggtagactt gacggccgct gctctggccc
    22981 ttgtgcggag ctcgaatatt cgctatatcg tagtgcaggc aggcgggggc ggcgagacgg
    23041 gctctttagg ccggcggctg ttcgaaagaa cacgcagcgg cgaactggac gagatggctt
    23101 cggaaaggga gctgcgcatc ttgcgcgtgc cgtaggctct ttccggcgac cgccggcgtg
    23161 gaccgccggt tttgtggcaa tcctgtgttt tgttagggat aggcctctgt ttgacgtact
    23221 tgaaaccgtc tgctagactg gtttggtaac cggagtcagc ccggaattgc gcctgtgaac
    23281 ctcacaacct gctatacttc tgccagctct ctgcgagttg caggcaggtt aaccctgcgc
    23341 acgctcactt caccctacgt gtttttggat atttacacag cgtccgggca agctccccgc
    23401 gtattcagtg gctatctgaa ttcccggttt gagctttaac acatgattgt gggtcaaaac
    23461 ttcagcagat gcaagcaaaa tccggcggcg gacgcgcgca ccgggcggct cttagtttga
    23521 cctgggtttc tgaatgccgt tcggctcttg aggagtaaat atggaaaatc cggcaaatgg
    23581 tgccatccgc gaacgagttg atttttcaaa gatcaagaca tcgattccga tcccgaattt
    23641 gatcgatgtt cagaaaaaat cctacgaacg ttttctgcag atggaactgc tacccgagga
    23701 gcgggatgat acggggctgc agagtgtctt cacgtcagtc ttccccatct cggatttccg
    23761 tggtttgagt cagctcgact tcgtcgatta ttcaatcgga aactgggagt gcaagtgcgg
    23821 caacctgaag ggtctgcacc accttcgaac tacctgccgg aatcccagtt gcggtgccag
    23881 aatcaggacg gaccctttcc acgccggtga tgtgttgtgt catcgctgcg gcacattcaa
    23941 ccgcaacatc gtgacattct gcaaccggtg cggagacccc gtatcgctgc agttgaaata
    24001 cgatgtcgcg gagtgcgaag agcggggcat gacctacgcc gcgccgctga aggtcaccat
    24061 ccggctgacg gtttacgaca aagacgcgga gaccggcaac aagtccgtcc gcgacatcaa
    24121 ggaacaggaa gtcttcttcg gcgagattcc gctaatgacg gaaaacggga ccttcatcat
    24181 caacggaacc gagcgtgtca ttgtctcgca gctacaccgc tcgcccggag ttttcttcga
    24241 gcgtgtgccg gcgcaaggct actacctcgg aaagatcatc ccgtaccgcg gcagttgggt
    24301 ggagttcgag tacgacgtca aaaacctgct ccacgtccgt atcgatcgca agcggaaatt
    24361 ctacggaacc gttttcctgc gcgcccttgg attcaagagc gacgagcaga tcctgcggac
    24421 cttctatacc atcgaaaagc tgaagatcaa ggacagcaag ctgttgtgga ccgtgtcccc
    24481 cggtctggtc ggtctgaagc tttcgcatgc catcaccgcc aaaaacggcg acacagtggt
    24541 tccacagggc cgcaagatca ccgcgtccgt tttccgcgac atcaataaga acaagatcga
    24601 gcaggtggaa gtcgcggcca acgatctcga aggcgcgtac gtcgccgccg acgtcatcga
    24661 catgtcgacg ggagaagtgc tgctcgaatc caatcatgag ctgacctcga gcggtatctc
    24721 caagatgatg gaagcgggca tcgaagagtt cgaggtgttc tttccggagc gcgacgaggt
    24781 cggcaatgtc atttccgtca cgctgaagaa ggactcggtc aagacacaga gcgacgcgct
    24841 gctggagatc taccgcaagc tgcggccggg cgacccgccg acgctcgata cggcgacgca
    24901 actgttccac ggcatgttct tcgatccgcg gaagtacgat ttttcgcgtg tggggcggat
    24961 gaagttcaac atcaagctct tcgaccgggc cgacgcaacc ccgctggata agcggacgct
    25021 cgatccggac gactttacgg gcaccattcg ctacctgttg aaattgcgca agggcatcgg
    25081 gctggtggac gatatcgacc acctggggaa ccgccgcgtc cgggcggtgg gcgaactgct
    25141 cgagaaccag ttccggatcg gcctggtgcg aatggagcgc gccatcaaag agaagatgtc
    25201 ggtctaccag gaaatgtcga cggcgatgcc gcacgacctg gtgaacgcga agcccgtgat
    25261 ggccgcgatc cgcgaatttt tcggatcgag ccagctttcg cagttcatgg accagacgaa
    25321 tcccttatcg gagatcacgc ataagcggcg gctctcggcg ctgggtccgg gaggattgtc
    25381 gcgagagcgt gcaggcttcg aagtgcgcga cgtgcatcct acgcactatg gacgtatctg
    25441 ccccatcgag acgccggaag gaccgaacat cggattgatt tcttcgctct cctgttttgc
    25501 gcgaatcaac gaatacgggt tcatcgagag tccgtaccgg cgcgtaaaag gcggcgtggt
    25561 ggtggaggaa gtccggatac tcaacccggg cgacagcaac catcgcgtcg gcgaagtgat
    25621 gacagcggcg gaggctgcca agatcaaaac cgatctggcc tccaggcaga ctttggaatg
    25681 ggaagcccac tgcgactatc tttccgcctg ggaagaagac aaatatacga tcgcacaggc
    25741 caacgtggag ctcgaccaga acgggcgcat ggtcaatgag cttgtcaacg cgcgagaggc
    25801 cggcgatttc gtactcactt cgcgtgacga agtggagtat atggacgtaa gtccgaaaca
    25861 gcttgtctcg gtagcggcca gtctcatccc cttcctcgag aacgacgacg cgaaccgcgc
    25921 gctgatgggg tcgaacatgc agaggcaggc cgttccgctg cttcgtgcgg aagcgccgct
    25981 cattggaacc ggtatggaac gggtgacggc gcgcgactcc ggggccgtgg tcatctgcaa
    26041 gcgctccggc gtcgtggatt cggtggattc cgagcgcatc attgtccgcg tcgagggtgc
    26101 ggctcacgaa gcccagcttt cgcgcgaagt gggcgccgac atctatcagc tcacgaagtt
    26161 caagcgctcc aaccagaaca cctgtatcaa ccagaagccg atcgtcttcg cgggccagcg
    26221 ggtaaagaag ggcgatgtgc tcgccgacgg cccttgcacg gatagcgggg agcttgccct
    26281 cggccgcaac gtcctggtgg cgttcatgcc atggcgcggt tacaacttcg aagacgccat
    26341 tgtggtcagc gaaaagctgg tcaaggaaga ctattacacc tcgatccaca tcgaggagtt
    26401 cgaaatcgag gctcgcgata cgaaactggg gccggaggaa attacgcgcg atatcccgaa
    26461 tatcgccgaa tccttcctcc gcaacctgga cgaaagcggc atcatccgaa tcggcgccac
    26521 agtcaagccg ggagacattc tggttggcaa agtgacgccg aagggggaaa cgcagcttac
    26581 tcccgaggaa aaactgctcc gggccatctt cggtgaaaag gccggagatg tgaaagacgc
    26641 ttcgctctac tgcccgcccg gaattgaggg cacggtggtg gactgcaagg tgttttcacg
    26701 caaaggagcg gagttggacg agcgctccaa gtggattctc cagtcgcaga tcgaccggct
    26761 gtaccggaac ctggaagacg agaagcgaat tctcaacgac gagcgcgcca agaggctggg
    26821 taatctgctg gaaggcaaac agcttcaggc cgacctgcac gacgagaaaa cgaaccggaa
    26881 gctgctctcc aagggagtcg acctgacgcg ggatatcgtg gagaagctcc gggctcgaga
    26941 cctgaagcgg atccggttga aggacaaaga tccgcgcctg aacgagcaga tcgacgagat
    27001 cgaggagatg acctcccggc agatttcggt cctcgagaag atcacggaag aacgcatcgc
    27061 caagttgagg aagggcgacg aactgccgcc gggcgtcatc aagctggtac gcgtctacat
    27121 cgccatgaag cgcaagctgt cggtcggcga caagatggcc ggccggcacg gcaacaaggg
    27181 tgtgattgcg cgcatcgttc ccgaagagga catgccgcac atgcccgatg gaacacccgt
    27241 cgagattgtc ttgaatccgc tcggcgtacc ctcccgtatg aacgtcggac agattctgga
    27301 gacccacctc gggtgggccg ccaaggcgtt gagccgccat ttcgcaaccc ctgttttcga
    27361 cggagcggct gagctcgata tcaaacacca gctcgaagaa gccggtctgc cggcttccgg
    27421 caaggtacaa ctggtggacg gcatgagcgg gctgccgttc gaacagcccg tgaccgtcgg
    27481 ctacatctac atgctgaaac tctcgcacct tgtggacgac aagattcacg caaggtcgat
    27541 cggaccgtat tctcttatca cccagcagcc gctgggcggg aaagctcaat tcggtgggca
    27601 gcgcttcggc gaaatggaag tctgggcgct tgaagcatat ggcgccgctt acgttttgca
    27661 ggaactgctg accgccaaat ccgacgatgt gtacggccgc gcaaagatct acgaagccat
    27721 cgtcaagggc gaagctgccg ccgagcccgg cgtgcccgaa tccttcaacg tcctgatccg
    27781 ggagttgcag tcgctttgcc tggacgttga actgatgaag aagccccgtg aagtgctgga
    27841 tacagcgctc gcggccgact gaggacattg aacttaccag cgatccatga cggaaacaag
    27901 gcgggcggtg aaggcccgcc tgctccgcct ttggtggctg gtggatcgat ggagaaacat
    27961 taggaggcta ttttgtacag atcctctccc tacgacagag ccaatttgat cgccgatttt
    28021 gattccattc gcatcagcct tgcgtcgccg gaaaagatcc gaagctggtc gcatggcgaa
    28081 gttaccaagc cggaaaccat caactaccgc acgtttaagc ccgagcggga cggcttgttc
    28141 tgtgcccgga tcttcggccc tgtcgccgat tgggagtgct tgtgcggcaa atacaagcgc
    28201 atgaagcacc gcggcgtaat ctgcgacaag tgcggtgtcg aagtaactct gtcgcgagtg
    28261 cgccgcgaac gccttgggca tatcgagcta gccagcccgt gttctcacgt atggttcttc
    28321 aagggcctgc cgagccggat cgggtacctg ctggacatca cgctccggga actggagcgc
    28381 gttctctact ttgaggcgtt cgtaattgtc gaccccggcg aggtgcccgg catttcgagg
    28441 ggcgaggtaa tcagcgacga gcgcaagcgc cagttggacc aggagcagcc cggtaagttc
    28501 gtcgccatga tgggcgccga gggcattaag gaactcctga agaagatcga cgtcgaatcg
    28561 ctgagcctgg aaatccgcga aaagatgaag accgaggcgt cacagcagaa gaagctgaaa
    28621 tacgccaagc ggcttcgcgt cgtggagtcg ttccgcaagt ccggaaataa gccggagtgg
    28681 atgattctgg acgttctgcc ggtaattccg ccggagttgc ggccgctggt gccgctcgat
    28741 ggcggccgct tcgccacctc cgacctcaac gacctgtacc gccgtgtcat caaccgcaac
    28801 aaccggttga agaagctgat ggaattgcac gcgccggatg tgatcgtccg caatgaaaag
    28861 cgcatgctcc aggaagccgt ggacgcactc ttcgacaacg gcaggcgggg ccgcgtgctg
    28921 cgcggtgcaa acaaccggcc gctcaaatcg ctgtccgata cgctcaaggg caaacagggc
    28981 cgcttccgcc agaacctgct cggcaaacgc gtcgactatt cgggccgctc ggtcatcgtg
    29041 gtgggaccgg agttgaagct gcatcagtgc gggttgccga agaagatggc gctggagctg
    29101 ttcaagccct tcatttatca tcgcctcgaa cagcgcggcc actgtaccac gatcaaacag
    29161 gcgaaagaac tggtggagca gcaggatccg gtcgtatggg acatcctcga ggaagtgatt
    29221 cgcgatcacc cgattctgct caaccgcgcc ccgacacttc accgtttggg tatccag
//
LOCUS       DASTMK010000212        12286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_137961, whole genome shotgun sequence.
ACCESSION   DASTMK010000212 DASTMK010000000
VERSION     DASTMK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137961"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFQ79_09480"
     CDS             complement(<1..192)
                     /locus_tag="VFQ79_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139930.1"
                     /translation="MPTSRRVFWSALAVYALGSFVAAAQTGSPWDVRPAHPVTSGTNL
                     LPDSPVLIKSLGDAAEYVLR"
     gene            complement(213..1385)
                     /locus_tag="VFQ79_09485"
     CDS             complement(213..1385)
                     /locus_tag="VFQ79_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU0139931.1"
                     /translation="MKPLTTATAVIILTAIMIQAETKPRARDLGIPFEGKAGPLNAIT
                     DVRGIEVAHTTLIRGEGPLKVGEGPVRTGVTVILPRGKESAQPVFAGWFSLNGNGEMT
                     GTTWVEESGLLEGPVAITNTHSVGTVRDAIIQWQVRRGTMYQDFSLPVVAETYDGWLN
                     DINGFHVKPEHVFSALEAASTGPVAEGNVGGGTGMNCYSFKGGIGTASRQVSTPVGSF
                     TLGVLVQANFGRRYQLKIAGAPVGQELTCDKWPCRSTGDAGPEAREGSIIVVAATDAP
                     LLPHQLKRIARRVSLGIARTGSSSGNGSGDIFIAFSTGNRDAGSTSSIPSVQVLPNAQ
                     MDALFEATTQATEEAIINAMVAAETMTGRDGHRSIALPHKELREALQKYNRLSTAF"
     gene            complement(1382..2206)
                     /locus_tag="VFQ79_09490"
     CDS             complement(1382..2206)
                     /locus_tag="VFQ79_09490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0139932.1"
                     /translation="MHVWSQDFTRYPVSPPEPGLRPITDEGSTEFYLQEMKQHGIDLA
                     VLVQPRQYGWDNRYIADSVRRFPRQFVGHGLIDPHDPDNAGVLEREVRVNGLAGMRLS
                     PIYHPKEQWLNAKANHALWRKAEELGAVFNVFIAAPQMAQLEDMVQRFPRVKVVVDHL
                     GRPEIIDRAPWAEDVSLLRLAKYSNVWVKFSELYIMSKTKKYPYADVHPFARSVYDAF
                     GPKRLLFATGMVGVTRRIPYADELRLVREDIPFFKARDKEWILGRNALQIWKWETA"
     gene            2325..4199
                     /locus_tag="VFQ79_09495"
     CDS             2325..4199
                     /locus_tag="VFQ79_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139933.1"
                     /translation="MFRSLGLFFFVLLTLSRAQSPGGPIVISTPMQPPEWALLERQLL
                     RANSEACERFAARYVDERGFLLHTPRWGTLNDGPDDAIETFYNWTLLHALGGSDSVLD
                     LFKKAYDGHLNQYKDVKSTSTDIARDGSYYKEFVPMSDWFHIGEGMRGVFFQGLSDPT
                     DERYRARMRRFAGFYMNEDPEAPNYDPKHRIIRSMWTGSKGPMLRKATTYDWVGDEIP
                     GRFHMLHGINATKEMRDMKEQYPKMHAHCKEYVDAVGDHPLNLASTSLALNAYMLTGE
                     KKYRDWILEYVGAWKERTDANGGNIPTNIGLDGRVGGEYGGKWYKGTYGWNFTIFDGE
                     IQQTAHRNTFDAGSWPGFGNAYLVSGDPGFIATLRRQIDNLYAHKKVVDGRVMLPSMF
                     GDPRGYGHSGREEWYNWTPNLRINRLTEIYMWSGDPNDLERVPKEGWLAFLAGGNPDF
                     PVKALRDDLNGIRSAVAAMREDTTTPDTRLADWPMRFNPARTDALVKLTLGGYLSGNI
                     WTLHSRLRYFDPVRRRSGLPEDVGALVEKLSGDSVTVMLVNLNQVEARTVAVQAGTYG
                     EHAFRSAAWNGRSQTLDGPVANVVLEPGSGARLELKMERYRNQPTLAQPWNRAWLHQQ
                     "
     gene            4169..5419
                     /locus_tag="VFQ79_09500"
     CDS             4169..5419
                     /locus_tag="VFQ79_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0139934.1"
                     /translation="MEPSLATPAVKQPSERLRWAVVVLLSLGMVIAYLDRVNLTVALP
                     SMSSGLGLTKAEQGLALSAIFWAYTALQIPCGMLVDRYGVRIPYMIGFLIWSLASAST
                     ALVGSLSSLVAARVLVGMGEAVVAPASMRYIGVHFAEKRRGLAVGLYMTGTKVGPAVG
                     LPLAAFLVSSFGWRLMFLMLGLACLLWLVPWLSWVGRDDPAATPRASSPGSKKPISLG
                     RLLSHPAVWGIILGTYSYMYFVYYSMTWMPLYLEERHGMSVRQMGWYSGVSFAGMALV
                     AALAGWAADRIIARGKDPITVRKAFTIAGLTAAASQTLVAFVDSPAVMLFFVVFSLCG
                     LGLATANYWALTHTLMPGASIAVVVGVQNTAANLAGIVAPWLTGWMIQQTGTFDAPIK
                     TVGVWLALGIGSYLLLVRRRFAPK"
     gene            complement(5432..6613)
                     /locus_tag="VFQ79_09505"
     CDS             complement(5432..6613)
                     /locus_tag="VFQ79_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0139935.1"
                     /translation="MQSRRTFFEMVSTLPLVGGLFASTGASRDFLKELGVKRIINGMG
                     VYTMATGSLMWPEAVEAIRSLSKTFVRLDDLHDAVGKRIAELLHCEAAMVPSGAAAGL
                     TVGTAACITGTDAAKILRIPDTAGMKNEVIVQKSHRFPYDHMVRNCGVRLVEVETRAE
                     LESAINARTAMLLFLNKANSQGQVKAEEFVAIGKARGIPTMNDAAADVPPVENLFKYT
                     KMGFDLVAFSGGKGIRGPQSAGLLLGRADLIRAARLNTAPHSDTIGRSCKVNKEELVG
                     MMVAVEMYLKHDHEADWREWERRIKVMADAVAGLPGVRTERFVPEIANQVPHLRIHLD
                     TARAGVTRPEVLQALRSEEPEIEVVPGSYVPDTIEIASWMLQPGEAEIVGRRLRKALR
                     G"
     gene            complement(6636..7925)
                     /locus_tag="VFQ79_09510"
     CDS             complement(6636..7925)
                     /locus_tag="VFQ79_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0139936.1"
                     /translation="MRYAFILMMAANLMNGQEFDLLLKGGHVIDPKNKIDAVRDVAIS
                     SGKIAAVAAGIPAAKAYKTIDVSGFYVTPGLVDIHAHFWGVYGKGIHPDSHTLRSGVT
                     TVVDAGGAGWRNFAAFKKDIVDRASTRVLAWLNIVGRGMDGRVAENDPADMDPAAAAK
                     TIRAHPGLIVGVKTAHYDGPEWVAVDRSLEAGNLAKVPIMVDFGTFRSERPFEELVTK
                     KLRPGDFYTHTYLNSVPMLDGEGHVRKFLFEARSRGVLFDAGHGQGSFLFRLAVPAVK
                     QGFVADTISTDLHTGSMNAGMKDMAMVMSKFLNIGVPLADLILRSTWNPARAIGHEEL
                     GHLSPGAPADIAVLRVEQGNFGFVDAYKARMTGSQRLVCEMTLRNGLVSWELNGISRE
                     PWDTLGDYGSQGDQRWDATAEDPPKQDRRPGRTRPKR"
     gene            8059..10728
                     /gene="aceE"
                     /locus_tag="VFQ79_09515"
     CDS             8059..10728
                     /gene="aceE"
                     /locus_tag="VFQ79_09515"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU0139937.1"
                     /translation="MLKTNVESAEDINPQETSEWVEALDQVIDESGPDRASFLLKRLL
                     SRASTLGVASQTSLNTPYVNTIPLEDELPYPGDRAVERKIKSFTRWNAMAMVVRANKY
                     DPGIGGHISTYASLATLVEVGFNHFFHAHYGNEPGDLVYFQGHASPGIYARAFLEGRL
                     SEKHLENFRHELRDEPGLSSYPHPWLMPGFWQFPTVSMGLGPINAIYQARFMRYLENR
                     GILAPSPRKVWAFLGDGETDEPESLGAITLPAREKLDNLVFVINCNLQRLDGPVRGNS
                     SIIQELESAFRGAGWNVIKVIWGDQWDDLLARDTSGLLVKRMGEVVDGEYQNYAARGG
                     AYVRKEFFGKYPELLDLVQDYSDDELGRLRRGGHDPKKVYNAYLRAIEHKGQPTVILA
                     KTIKGYGMGEAGEGRNVTHQQKKLNEQEMENFRRRFEVPIPDKAVHTTSFYRPPDDSQ
                     EMKYLRQRREELGGYLPARDPKPIEIKAPALEVFSDTIAGSGGREASTTSACVAILKS
                     LLKTDLGKYVIPVVPDEARTFGMESLFRQIGIYASQGQLYKPVDREMILYYKEAKDGQ
                     ILQEGITEAGSMASFTAAGTAYSNYGVTMIPFFIYYSMFGFQRIGDLIWAFGDARGKG
                     FLVGGTAGRTTLAGEGLQHQDGHSHVLSSTVPSCRSYDPAYAYELAVIVQDGIRRMYQ
                     ENENHFYYLTVYNENYAQPAMPEGVQEGILRGIYRYRAATGAKASVQLFGSGPILNEA
                     LRAQELLAERFQIPADVWSVTSYNQLRRDALEVERWNRLHPTETERKPYLLEALEGAE
                     GPVIAATDYMKVVADQISPWLPGRLVSLGTDGFGRSDNRQYLRKFFEVNAESIAGYAL
                     ARLARDGKFDTGKAQAAMRELGLDPDSADPATL"
     gene            10756..11742
                     /locus_tag="VFQ79_09520"
     CDS             10756..11742
                     /locus_tag="VFQ79_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEU0139938.1"
                     /translation="MLFLSEADVRNLLPMARAIDLLREGFDAYASDRAQNQPRRRLIL
                     PSGASLHSMAGAYGSYFGTKIYSNHPKHKTWFLFVLYNAETATPLSLFEADILGQIRT
                     GAASGLAADLLAPAQADTVALIGSGFQARGQLDAMRVVRPVRKVRIWSRSAERRERFA
                     AECAAAGLEAHVASSAADAIAEAQIVVTATFAKDPVLESEAITAGTFIAAMGSNHPQR
                     RELPAELIQRAGLLVADSIEQARLEAGDFLLALDGDGWRRVVELRSVVSTPDRYAHCV
                     DGITIFKSVGLGLEDVIAAGFVYEEAKRLGMGSELPIFIPEPKSESRYPATR"
BASE COUNT         2381 a         3677 c         3698 g         2530 t
ORIGIN      
        1 gcgcaacaca tactcggctg cgtcaccgag cgatttgatg aggaccggcg agtccggcag
       61 caggtttgtt cccgacgtca cgggatgggc aggccttaca tcccacggac tgcccgtctg
      121 ggcggctgca acaaacgaac cgagggcata aacggccagg gcagaccaaa agacacgtcg
      181 cgatgtaggc atgttcaatt caagatactc tgtcagaacg ccgtggacag gcggttgtat
      241 ttttgtaacg cctcccgcag ttctttgtgc ggcagcgcga tgctgcggtg tccgtcccgg
      301 cctgtcatcg tttcggcggc aaccatcgca ttgatgatcg cttcttccgt ggcttgcgtc
      361 gtcgcctcga acaaggcgtc catttgagca ttcggaagta cctggacgga agggatggag
      421 gaagtggagc ccgcatcccg gttgcctgtg gagaaggcaa tgaagatatc gccggaaccg
      481 tttccagagc tgcttccggt gcgtgctatg ccgagagaaa cccggcgcgc gatgcgcttc
      541 agttgatgcg gcagcagcgg cgcatcggtc gccgcgacaa cgatgatcga gccctcgcga
      601 gcctcgggtc cggcatcgcc ggtagaacgg cacggccact tgtcgcacgt cagttcctgc
      661 cctaccggcg cgcctgcgat cttcaactgg tatcgccggc cgaagttggc ctggaccagc
      721 acgccaagcg tgaaggagcc caccggagtc gatacctggc gcgatgccgt tccgataccg
      781 cctttgaacg aatagcagtt catgcccgtt ccgccgccaa cgttcccctc cgcgacaggt
      841 ccggtcgaag ctgcctccag cgcggagaac acgtgttccg gcttgacgtg aaacccgtta
      901 atgtcgttca gccatccgtc ataggtttcc gccaccaccg gcaaagagaa gtcctggtac
      961 atcgtgccgc ggcgcacctg ccactggatg atggcgtcac gcaccgtgcc tacgctatgt
     1021 gtgtttgtga tggcgaccgg accttcgagc aggccggact cttccaccca cgtcgtgccg
     1081 gtcatctcgc catttccgtt caaggaaaac cagccggcga atacaggttg agcagactcc
     1141 ttgccgcgcg gcagtatcac cgtaacgccc gtgcggacgg ggccttctcc aaccttcaac
     1201 ggtccctcgc cgcgaatcaa tgtagtgtgg gccacttcaa ttccgcgaac gtctgtaatg
     1261 gcgttcaacg gaccggcctt cccttcaaag gggataccca aatcgcgtgc gcgtggtttt
     1321 gtttctgcct gaatcatgat cgcagtcaga atgataacgg ccgttgcggt agtcagtggt
     1381 ttcatgccgt ctcccacttc cagatctgaa gcgcgttccg gccgagaatc cattccttat
     1441 cgcgtgcctt gaagaacgga atatcctcgc gcacgagccg caattcatcc gcatagggaa
     1501 ttcgccgcgt gactcccacc attcccgttg caaacaacag ccgtttcggt ccgaaagcgt
     1561 catagacgct gcgcgcgaac ggatgcacgt ccgcataggg atactttttc gtcttggaca
     1621 taatgtacag ctcggagaat ttcacccaga cattcgaata tttcgcaaga cggagcaggg
     1681 aaacgtcttc cgcccacggc gcacgatcga tgatctccgg ccgccccaga tgatcgacaa
     1741 caacttttac gcgcggaaac cgctgcacca tgtcctccag ttgagccatc tggggcgcag
     1801 cgatgaatac gttgaagacg gcgcccagtt cctctgcctt tcgccatagg gcgtggttcg
     1861 ctttcgcgtt cagccactgt tcctttgggt ggtagatggg gctgagccgc atacccgcca
     1921 ggccgttcac ccgcacttcg cgttcgagca cgccggcgtt gtccggatcg tgagggtcga
     1981 tgagaccgtg gccgacgaac tgccgcggaa atctccggac gctgtcggcg atgtagcggt
     2041 tatcccatcc gtattgccgc ggctgtacaa gcaccgccag gtcgatgcca tgctgcttca
     2101 tctcctggag ataaaactcg gtgcttcctt catcggtgat ggggcgaagg cccggttcgg
     2161 gcgggctcac cggataacgc gtaaagtcct gggaccacac gtgcatatgt gtgtcgataa
     2221 ctggagactg cggcagagct gcccaggccg cgccgccgaa caaaagttcc cgtcgtgaaa
     2281 ggtttggaat ttgcacaatc gttcccttcg catatgataa atgcatgttt cgatccttgg
     2341 ggctgttttt tttcgtgcta ctgacgctgt cgcgggctca atcccccggc ggtccgattg
     2401 ttatctccac tcccatgcag ccgccggaat gggcgctgct ggagcgccag cttctccggg
     2461 ccaactcgga ggcgtgcgag cgttttgccg cgcgctacgt ggatgagcgt ggttttctgc
     2521 tgcacacgcc acgctggggc actctgaacg acggccccga cgatgccatc gagactttct
     2581 acaactggac cctccttcac gcgctgggtg ggtcggattc cgtcctcgat ctgttcaaga
     2641 aagcctatga cgggcacttg aaccaataca aggatgtgaa gagcaccagc accgacatcg
     2701 cgcgcgatgg gagctactac aaggaattcg ttcctatgtc cgactggttc catatcggcg
     2761 agggtatgcg cggcgttttc tttcagggcc tgtcggaccc aaccgacgaa cgctaccgcg
     2821 cgcgcatgcg gcggttcgcc ggtttctata tgaatgaaga ccccgaagct cccaattacg
     2881 acccgaagca ccgcatcatt cgaagtatgt ggaccggaag caagggaccg atgctgcgca
     2941 aggcaaccac ctacgactgg gtaggcgacg aaatccccgg acgcttccac atgttgcatg
     3001 gcatcaatgc cacgaaagag atgcgcgaca tgaaagagca gtatccgaag atgcacgcgc
     3061 actgcaaaga atacgtagac gcggttggcg accatccgct gaatctcgca tccaccagcc
     3121 tggcactaaa tgcttacatg ctgacgggtg agaagaagta tcgcgactgg atcctggagt
     3181 atgtgggcgc gtggaaggag cggacggatg ccaacggcgg caacatcccc accaacatcg
     3241 gactggacgg gcgcgtcggc ggagaatacg gcggcaagtg gtacaaaggc acctacggtt
     3301 ggaacttcac gattttcgac ggggagattc aacagaccgc gcatcggaac actttcgatg
     3361 ccggaagctg gccgggtttc ggaaacgcat acctggtgag cggcgacccc ggtttcatcg
     3421 cgacgttgcg gcggcagatc gacaacctgt acgcgcacaa gaaagtagtc gacggcaggg
     3481 taatgctccc gtccatgttt ggcgacccgc gcggctacgg tcatagcggg cgggaggaat
     3541 ggtacaactg gacgccgaac ctgcgcatca accgcttgac ggaaatctat atgtggtccg
     3601 gcgatccgaa cgatctggag cgtgtgccga aagaaggatg gctcgctttt cttgcgggcg
     3661 gcaacccgga ttttcccgtg aaggcgctgc gcgatgactt gaatggaatc cggtcggctg
     3721 tggccgccat gcgcgaagac acgaccacgc ccgatacgcg gctggccgat tggcccatgc
     3781 gcttcaaccc ggcgcgcacc gacgcgctgg tgaagctcac gctcggcggg tatttgtcag
     3841 gaaatatctg gacgctgcac agccgcctgc gctatttcga tcccgtgcgc aggcgctccg
     3901 gcctgccgga ggatgtaggc gcgctcgtcg aaaagttgag cggcgactcg gttacggtta
     3961 tgctggtgaa cctgaatcag gttgaggcgc ggaccgttgc ggtgcaggca ggcacctatg
     4021 gcgagcacgc atttcgctcg gctgcctgga acggacgctc gcagacactc gacggcccgg
     4081 ttgccaatgt ggtgctggag ccgggttccg gcgcccggct cgagttgaaa atggaacgtt
     4141 atcgcaatca gccgacgttg gcccaaccat ggaaccgagc ctggctacac cagcagtaaa
     4201 acagccgtcc gagagacttc gctgggccgt tgttgtcctg ctgagtctgg gcatggtcat
     4261 cgcctatctg gatcgtgtca acctcaccgt cgctcttccg tcgatgtcga gcggactcgg
     4321 gctgacgaag gcggaacagg gtcttgccct gtcggccata ttctgggcgt acacagctct
     4381 tcaaattcca tgcggcatgc tggtggaccg ctacggcgtc cgcattccct acatgatcgg
     4441 atttctgatc tggagcctgg cgtcggccag tacggcgctg gtgggcagcc tttcctcact
     4501 ggttgccgca cgcgtgctgg tgggaatggg cgaggcggtc gtagcgccgg ccagtatgcg
     4561 gtacatcggc gtgcatttcg ccgagaagag aagaggattg gccgtcggcc tctacatgac
     4621 gggcaccaag gtcgggcctg ccgtgggtct gcctctggcg gcttttctgg tttcgtcctt
     4681 cggatggcgt ttgatgtttc tgatgctggg cctggcgtgc ctgctctggc tggtgccatg
     4741 gctttcctgg gtgggccggg acgacccggc ggccaccccg cgagcctcca gtccgggatc
     4801 gaagaaaccg atctcgctcg gccgtctgct ctcccacccg gcggtttggg gcatcattct
     4861 cggaacctac tcctacatgt actttgtcta ctattcgatg acgtggatgc ccctgtatct
     4921 cgaagagcgt cacggaatga gtgtgcggca gatgggctgg tacagcggcg tgtcctttgc
     4981 gggaatggcg ctggtggcgg cgcttgccgg atgggcggcg gaccggatca tcgcgcgggg
     5041 aaaggatccg ataacggtcc gcaaagcgtt cacgattgcc ggactgaccg ccgccgcgtc
     5101 tcagacgctg gttgcctttg tggattcacc cgcggtcatg ctgttcttcg tggtgttctc
     5161 gctgtgcggc ctgggcctcg cgacggccaa ctactgggct ttgacgcaca cgctgatgcc
     5221 cggagcgagc atcgcggttg tcgtaggcgt gcagaacacc gcggcgaacc tcgcaggcat
     5281 cgtcgctccc tggctcaccg gatggatgat ccagcagacg ggaacgttcg acgctcccat
     5341 caaaaccgtc ggggtgtggc tggcgctcgg gattggctcc tacctgctgc tggtgcgccg
     5401 ccggttcgct ccgaaatagc gcccgtccgt ttcagccgcg aagcgcttta cggagccggc
     5461 ggccgacaat ctccgcttcg ccgggctgga gcatccagga tgcgatctca atggtatccg
     5521 gcacatagga gcccggcacc acttcaattt cgggctcttc gctgcgaagc gcctggagga
     5581 cctcgggccg tgtgacgccg gcccgcgcgg tatccaggtg aattcgcagg tgcggcacct
     5641 ggttggcgat ctccgggacg aagcgctccg tgcgaacgcc gggcaatccc gccacggcat
     5701 ccgccattac tttgattcgc cgctcccact cccgccagtc ggcctcatgg tcgtgcttca
     5761 gatacatttc aacggcgacc atcatgccta ctagttcttc tttgttgacc ttgcagcttc
     5821 gtccgatggt gtcgctatgc ggtgcggtat tcagccgggc ggcgcgaatc agatcggctc
     5881 ttccgagcag caggcctgcg ctctgcggac cgcgaattcc cttgccgccc gaaaaagcga
     5941 ccaggtcgaa acccattttc gtgtacttga acaggttttc gacgggcgga acatccgccg
     6001 cggcatcgtt catggtggga atgccgcgcg ccttgccgat ggcgacaaac tcttccgctt
     6061 tcacttgccc ctgtgaattg gctttattca ggaagagcag catggcggtg cgggcattga
     6121 tggcgctctc gagctccgct cgtgtttcca cttccaccag gcgcacgccg cagttccgca
     6181 ccatgtgatc gtaggggaac ctgtgtgact tctgcacgat tacttcgttc ttcatgcctg
     6241 cggtgtcggg aatgcggagg atcttcgcgg cgtctgtccc ggtgatgcag gcggcggtgc
     6301 ctacggtgag gcccgcggcg gcgcctgaag gcaccatggc cgcttcgcaa tgaagcagtt
     6361 ccgcgatgcg cttgccaacg gcgtcgtgca gatcgtccaa tcgcacaaac gtcttcgaca
     6421 gcgacctaat cgcctccacg gcttccggcc acatgaggga acccgtggcc atcgtgtaga
     6481 ctcccatgcc gttgatgatg cgtttaacgc cgagctcttt cagaaaatca cggctcgcgc
     6541 cagtggaggc gaacagtccg cctaccagcg ggagtgttga aaccatttcg aagaacgttc
     6601 tcctgctctg catccttgtg cctccttccg cttcctcacc gcttgggtcg cgtgcgaccc
     6661 ggccgccggt cctgcttcgg cggatcttcg gcggtcgcat cccatctctg gtcgccctgc
     6721 gagccatagt cgcccagcgt atcccagggc tcgcgcgaga ttccgttcag ctcccacgac
     6781 accaggccgt tgcgcagcgt catttcgcaa accagcctct ggctgccagt catccgcgct
     6841 ttataggcgt ccacgaatcc gaaattcccc tgttcaacac gcagcaccgc gatatcggcg
     6901 ggggctccgg gggacagatg gcccaactcc tcgtgtccga tggcgcgagc gggattccac
     6961 gtcgaccgca ggatcaagtc cgccagtggc acgccgatat tgagaaactt cgacatgacc
     7021 atggccatgt ctttcatacc ggcattcatg cttccggtgt ggagatcggt ggagatggtg
     7081 tcggccacga agccctgctt tacggccggt accgcgagcc ggaacaagaa gcttccctgt
     7141 ccgtggccgg catcgaacaa tacgccgcgg ctgcgcgctt cgaacaggaa cttgcggaca
     7201 tgcccttcgc cgtccagcat cggcacgctg ttgagatagg tatgcgtgta gaaatcgccc
     7261 ggccggagtt tcttcgttac cagctcctca aagggccgct cggagcgaaa cgttccaaag
     7321 tccaccataa tggggacctt ggcgagattg ccggcttcga gcgaacggtc tacggccacc
     7381 cattcgggac cgtcgtagtg cgcggttttc acaccgacaa tcagcccggg atgggcgcga
     7441 atcgttttgg ccgccgctgc cgggtccata tcggctggat cgttttcggc gacgcgtccg
     7501 tccattccgc gccccacgat attgagccag gccagaacgc gcgtggaggc gcgatcgacg
     7561 atgtctttct tgaaagcggc aaagttgcgc cagccggcgc ctccggcgtc cactacggtg
     7621 gtgacgccgc tgcgaagggt gtggctgtcc ggatggatcc cttttccata tacaccccag
     7681 aagtgcgcgt gaatatccac caggcccggg gtcacgtaga acccggacac gtcgatggtc
     7741 ttgtaagcct tggcagcggg gattccggcg gccaccgccg cgatcttgcc ggacgatatg
     7801 gcaacgtccc ggaccgcatc gatcttgttt ttcggatcga tgacgtgtcc gcctttcaga
     7861 agtaaatcga attcctgtcc gttcatgaga ttggcagcca tcatcagtat gaatgcgtac
     7921 cgcaaagatc ctatcctcat catccgcccc ctgcctggag aggcacctgt ccgctccggc
     7981 tcccctccga tgatagaata agatgtatcc taacaccgcg gaagagcggg ctatcttccg
     8041 tacggaacaa tcgccaacat gctgaagact aacgtagaaa gcgccgaaga tatcaatcca
     8101 caagagacct cggagtgggt tgaagcgctt gatcaggtga tcgatgaatc cggccccgat
     8161 cgcgcttcct tccttctgaa acgtctcttg agccgggcct caaccctggg ggttgcttct
     8221 cagacgagcc tgaacactcc ttacgtcaat acgattccgc tggaagacga gctgccgtat
     8281 cccggtgaca gggctgttga acggaagatc aagagcttca cgcgctggaa cgcgatggcc
     8341 atggtggtcc gggcaaataa atacgatccg ggcatcggtg gccatatctc cacgtacgcc
     8401 tctctggcaa ccctggtcga agtggggttc aaccatttct tccacgccca ctatgggaac
     8461 gagccaggcg acctggtgta tttccagggc cacgcctctc ccggtattta cgctcgtgcc
     8521 ttcctggaag gcaggctgtc cgagaagcac ctggaaaact ttcgtcatga gttgcgcgac
     8581 gaaccgggct tgtcctctta tccacaccct tggctgatgc cgggtttctg gcagtttccg
     8641 acggtctcca tggggctcgg cccaatcaac gccatttacc aggcgcgttt tatgcggtac
     8701 ctggagaacc ggggaattct tgcacccagc ccgcgaaagg tctgggcatt tctcggggac
     8761 ggcgaaacgg acgaaccgga atccctcggc gccatcacgc tgccggcacg cgaaaagctg
     8821 gacaacctgg tctttgtgat caattgcaat ctccagcgtc tggacgggcc ggtccggggc
     8881 aacagcagta tcattcagga actcgaatcc gctttccggg gcgcgggatg gaacgttatc
     8941 aaagtgatct ggggcgacca gtgggacgat ctgctggcgc gcgacacgtc cgggctgctg
     9001 gtgaagcgca tgggcgaagt tgtggacggc gagtatcaga actacgccgc ccggggtgga
     9061 gcttacgtcc gcaaggagtt tttcgggaag tatccggaac tgctcgatct cgtgcaggat
     9121 tactccgacg acgagctggg aagactgcga cgtggagggc atgatccgaa gaaggtctac
     9181 aacgcctatc tgcgcgccat cgagcataaa ggccagccga ccgtcattct cgccaagacc
     9241 atcaaaggat atgggatggg cgaggcgggt gaagggcgga acgtcaccca tcaacagaaa
     9301 aaattgaacg agcaggagat ggagaacttc cgccgccggt tcgaagttcc aatccccgac
     9361 aaggccgtgc acacgacatc gttttaccgg ccgccggatg attctcagga gatgaaatac
     9421 ctgcggcagc ggcgtgagga actcggcgga tatctgccgg cgcgcgaccc caaacccatc
     9481 gagatcaagg cgcccgcgtt ggaagtgttt tcggatacca tcgctggctc cggcggccgc
     9541 gaagcttcca ccacttcggc ttgtgtggcg atcttgaaat cgctgctgaa gacggacctc
     9601 ggcaagtatg tgatcccggt ggtgccggat gaggcgcgca cgttcggcat ggagtcgctc
     9661 tttcggcaga ttggcatcta cgccagccag ggccagcttt ataagcccgt ggatcgcgag
     9721 atgatccttt attacaagga ggccaaggac ggccagatcc tgcaggaagg catcacggaa
     9781 gccgggtcca tggcgtcgtt caccgccgcg ggtacggcgt attcgaacta cggcgtgacg
     9841 atgattccgt tcttcatcta ctactccatg ttcggattcc agcgtatcgg cgatctgatc
     9901 tgggcgtttg gcgatgctcg cggaaaggga tttctggtcg gaggaaccgc cggacggacc
     9961 accctggcgg gcgaagggct acagcaccag gacgggcata gccacgtcct ttcgagtacc
    10021 gtgccgtcgt gcagaagcta tgatcccgcg tacgcttacg agctggcggt catcgttcaa
    10081 gacggcatcc gccggatgta ccaggagaac gaaaatcact tctactacct gacggtctac
    10141 aacgagaact atgcccagcc ggcgatgccg gagggcgtcc aggaagggat ccttcgcggc
    10201 atctatcggt atcgcgccgc gacgggagcg aaggcgtccg tgcagctttt cggcagcggt
    10261 cccattttga acgaagcgct gcgcgcacag gagctgcttg cggagaggtt tcagatacct
    10321 gccgatgtgt ggagcgttac cagctataac cagctccggc gcgatgcgct cgaagtagaa
    10381 cgatggaacc gtctgcatcc tacggaaacc gagcggaagc cctacctgct ggaagcactc
    10441 gaaggtgctg aggggccggt gatcgccgcc accgactaca tgaaagtggt agcggaccag
    10501 atttcgccct ggctgcccgg caggctggtg tcgctcggta ccgatggatt cgggcgcagc
    10561 gacaatcgtc aatatcttcg aaagttcttc gaagtaaatg ccgagtccat cgcaggctac
    10621 gcgctggccc ggctggcgcg agacggaaaa ttcgataccg gaaaggctca ggctgccatg
    10681 cgcgagctcg ggcttgatcc ggattccgcc gatccggcaa cgctttaggc tggacgccgc
    10741 gcggcttctg ttcccatgct cttcctcagc gaagcggatg tccggaatct cctgccgatg
    10801 gcacgtgcca tcgatctgct gcgcgagggt tttgacgcct acgcttcgga tcgcgcgcag
    10861 aaccagccgc ggaggcggct gattctgcca tcgggggcat cgctgcactc catggctgga
    10921 gcgtacggct cctatttcgg cacgaagatc tattcgaatc atccaaagca caagacctgg
    10981 tttcttttcg tgctctataa tgcggagacc gccacgcctt tgtcgctgtt tgaggcggat
    11041 atcctcggcc agattcgaac cggtgcggcc agcgggctcg cggccgatct gctggcgccg
    11101 gcgcaggcgg acaccgtcgc cctgataggg agcggattcc aggcgcgtgg ccagttggac
    11161 gccatgcggg tggtgcgtcc ggttcgcaaa gttcggattt ggagccgcag tgcggagcgc
    11221 cgggagcgat tcgcggcgga gtgtgccgcc gccggactcg aggcgcatgt tgcatcttca
    11281 gcggcagatg ccatcgctga agcgcagatc gtcgtcaccg cgacctttgc gaaggacccc
    11341 gtattggaat ccgaagcgat cacggcaggt acgtttatcg ccgctatggg ttcaaaccac
    11401 ccgcaacgcc gcgaactacc cgcggagctg attcagcgtg ccggcttgct ggtggcggat
    11461 tccatcgaac aggcgcggct cgaagcgggt gacttcctgc tggcattgga cggcgacgga
    11521 tggcggcggg ttgtcgaact gcgatcggtc gtcagcacac ccgaccgcta tgctcactgt
    11581 gtcgacggaa tcactatttt caaatcggtc ggtttaggcc tggaagacgt aatcgccgct
    11641 ggattcgttt acgaggaagc gaaaagactg ggaatgggga gcgaactccc catttttatt
    11701 cctgagccga aatctgagag tcgttatccc gccacgcgct gattttcagc cgccggcaga
    11761 cccagcatag attttcgacg gagtttgggg cgcattccgc tccacagacc gagcatttct
    11821 ccacccgttg aatcttatcc gtatagacgg atttcttagc gttttccaga atctcctggg
    11881 agatcacggg ttccggcgcc ggctgggttt tgcgtttcat caatcgcccc taacaccaat
    11941 agaccatact ggcacgaaca gaacaacccc cggtgtacta ggctgcctgt tccaggcgga
    12001 ctacgtaccc tgcatccagc gaggaaccac accgtgagca cttctgcccg gatttggctc
    12061 ttgccgattt accctgccat accacctgtg cttccacagt gtgagtacat agataacagt
    12121 agacaaatcc tttccgttcg ttgctcgccg ctgctgtttt cattggcacg ctcattgtct
    12181 cctccattga aatcggttca gtcagtacaa cagacgcagt tcgagggcca aaagatttgg
    12241 aaaattcgta aagattctta aactcgcaag tgtaggaaga acatca
//
LOCUS       DASTMK010000213         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_393873, whole genome shotgun sequence.
ACCESSION   DASTMK010000213 DASTMK010000000
VERSION     DASTMK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393873"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..983
                     /locus_tag="VFQ79_09525"
     CDS             117..983
                     /locus_tag="VFQ79_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0139939.1"
                     /translation="MYKLLFTSLLAAGMMFAQAGKIRVIAIGAHPDDCDLGAGGLAAK
                     FAEMGHAFKFVSVTNGDAGHQEMGGGALAKRRRAEAAEAGRRLGIEYELLDNHDGELL
                     PTLDVRLDIIRRIRSWNADVVIAPRPNDYHPDHRYTGVLVQDAAYMVVVPNVAPDTPP
                     LRKNPVFLYFQDHFQKPAPFRPDIAVAIDDVYAKKVDALDAHVSQFYEWLPWVDGRLD
                     EVPKDPVERKKWLFNQRQRRDVTPAIRQSLIKWYGEQRGKQVRHAEAFEICEYGKQPD
                     EQEIRRLFPMLK"
     gene            1048..3135
                     /locus_tag="VFQ79_09530"
     CDS             1048..3135
                     /locus_tag="VFQ79_09530"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEU0139940.1"
                     /translation="MFLGFLAFTAALPSRAAEAFRIVTLSNQPDKISGGDVLVRIDVP
                     EAESPAGFTVKLNGRDVTTAFHRHPSLPVFTGLVTGLRKGQNSLEVFSGGESPSAMIS
                     LTNFDRAGPVFSGPHQQPFICETENFKLPDGSLLGPPLDENCSVETVVTYVYKPDADS
                     APVKPLGSRSVLPPDVAWTTTTSGLRVPYIVRVETGTINRGIYQTAILHDPTTEPEPG
                     PLAPPKAWNGRLLYSFGGGCTGGWYRQGGRLQSLLSDQIVGKGYAEASSTLNVFGMNC
                     QDVTAAETMMMVKERFIEAYGRPLFTFGRGGSGGSYQQLQIADGYPGLLDGIIPSATF
                     PEVLETTQFLVDAQLLDNYFNNAGQALTEEQRMAISGAGTMKNVTATAFGAGRIHPSK
                     FCPEVLPPELRYDPVLNRAGARCDIFDHTVNVYGRDPVTGFARRVIDNVGVQYGLGAL
                     NKGIITPAQFLDLNEKIGGYDNDGNIVATRAVADLQAVRAAYQTGRITNGGGGLASLP
                     IIDYRVYMDLGERGDVHLKYHSFALRERLQAANGTTANEVMLVVPPGTGSRAVHDYAI
                     TKMDEWLTNLTNDTSNDPAMDKIVRAKPQDLVDSCYTATGERIVETQTFSGGECNKLY
                     PAFPSPRMVAGGPITNNVLKCQLKPVDFAGYSAAFTETEQERLRSVFPDGVCDWSKPG
                     LEQQKPLGTWLRY"
BASE COUNT          679 a          963 c          995 g          659 t
ORIGIN      
        1 atgttgccga gcggtcggcg cacagcacga tgatcgctct gctcggccgt gaagcaatct
       61 acagtggaaa ggaacagacc tggaagggac tatttggcgc ttagtgcgaa attaccatgt
      121 acaagctctt atttacttcg ttgctggctg ccggtatgat gttcgcccaa gccggtaaaa
      181 tacgtgttat tgctatcggg gcccatcccg atgattgcga cctgggcgcc ggcggactcg
      241 cggccaagtt cgccgagatg gggcatgcat tcaagtttgt atccgtgact aacggcgatg
      301 ccgggcacca ggagatgggc ggcggtgcgc tggcgaagcg gcggcgcgcc gaagcggcag
      361 aagccggccg gcgtctcggc atcgagtatg aactgctgga caaccatgac ggcgaactgc
      421 tgccgaccct cgacgtgcgc ctcgacatca tccgccgcat tcgcagttgg aatgccgacg
      481 tcgtaattgc gccgcggccc aacgactatc atcccgacca tcgctacacg ggcgtgctgg
      541 tgcaggacgc ggcatacatg gtggtggtac cgaatgtggc tcccgataca ccgccgctgc
      601 ggaagaaccc cgtgttcctc tatttccagg atcattttca gaagccggca ccgttccggc
      661 cggacattgc ggtcgctatt gacgatgtgt acgcgaagaa agtcgacgcc ctcgacgccc
      721 acgtatcaca gttctacgaa tggctgccct gggtggatgg acgtctcgac gaggttccca
      781 aagatccggt cgagcggaag aagtggctct tcaaccagcg ccagcggcgg gatgtcactc
      841 ccgcgatccg gcaatccctc atcaagtggt acggcgagca gcggggcaaa caggtgcggc
      901 acgcggaagc cttcgagatc tgtgaatacg gcaagcagcc ggatgagcag gaaattcgcc
      961 gccttttccc gatgttgaag taaccggtcc acttcatacg cccacagatc gcgtcgtatg
     1021 tgcgttcgtt tggttttcag gctcatagtg tttcttgggt ttctggcttt cacggccgct
     1081 ctcccatctc gggcggccga ggccttccgg atcgtaactc tctccaatca gcccgacaag
     1141 atcagcggcg gtgacgtcct cgttcgcatc gacgtgccgg aggccgaatc gccggcgggc
     1201 ttcacggtca agctgaacgg acgggacgtc acgacagcgt tccaccggca cccttcgttg
     1261 cccgtattca ccgggctggt gacaggactg agaaagggac aaaactcgct agaggtgttc
     1321 agtggtgggg agagcccgtc cgcgatgatt tcgctgacaa acttcgatcg cgccgggccg
     1381 gtcttctcgg gcccgcacca gcagccgttc atttgcgaaa cggagaattt caaactcccg
     1441 gatggaagcc tgctcgggcc tcctctcgac gagaactgtt ccgttgagac ggtggtcact
     1501 tacgtataca agccggacgc ggattcagct ccggttaagc cgctgggctc ccgttctgtt
     1561 ttgccgccgg acgttgcgtg gaccacaacc accagtggtt tgagagtgcc gtacatcgtc
     1621 cgggtggaaa ccgggaccat taaccgtgga atctaccaga ccgccattct gcacgatccg
     1681 actacagaac cggagcctgg accgctggcg ccaccgaaag cctggaacgg gcgattgctc
     1741 tactctttcg gtgggggctg tacgggcggc tggtacaggc agggaggcag gctgcaatcg
     1801 ctcctcagcg accagatcgt cggcaaaggt tacgccgagg cctcatccac gctcaatgtc
     1861 ttcggcatga actgccagga tgttaccgcc gccgaaacca tgatgatggt gaaggagcgc
     1921 ttcatcgagg cttacggacg gcccttgttt accttcggcc gcggcggctc cggcggatcg
     1981 taccagcaat tgcagatcgc tgatggttac ccgggcctgc tggacgggat tattcccagc
     2041 gccacgtttc cggaagtgct ggaaacgacg cagttcctgg tggacgctca actgttggac
     2101 aactatttca acaacgcggg ccaggcgctc accgaagagc aacggatggc gatctccggc
     2161 gccgggacga tgaagaacgt gaccgctacg gcattcggcg cgggccggat tcatccgtcg
     2221 aaattctgtc cggaagtact gccgccggaa ctgcgctatg accccgtgct gaaccgtgcg
     2281 ggagcccgtt gcgatatttt cgatcacact gtaaacgtgt acggccgcga ccccgtgacg
     2341 ggttttgcgc gccgcgttat cgacaacgtt ggtgtgcaat atggtttggg ggcgctgaac
     2401 aaaggcatca tcacgcccgc tcagttcctg gatctcaacg aaaagatcgg cggctacgat
     2461 aacgacggaa atattgtagc cacgcgcgcc gtcgccgacc ttcaggccgt gcgcgcagct
     2521 tatcagacgg gcaggatcac gaacggcggc ggcgggctgg caagcctgcc gatcatcgac
     2581 taccgagtat acatggacct cggcgaacgg ggcgatgtcc atctcaagta ccattccttc
     2641 gcgctgcggg aacgtttgca agccgccaat ggcacgacgg cgaatgaagt aatgcttgtc
     2701 gtgccgccgg gaacaggttc ccgcgcggta cacgattacg cgattaccaa gatggatgag
     2761 tggttgacca acctcacaaa cgacacttcc aacgatccgg cgatggacaa aatcgtccgg
     2821 gctaagccgc aagaccttgt ggattcgtgc tacacggcga ctggcgaacg catcgtagaa
     2881 acgcaaacgt ttagtggcgg ggaatgtaac aagctgtacc cggccttccc ttctcctcga
     2941 atggttgccg gtggtccgat caccaacaac gtattgaagt gccaactgaa accggttgac
     3001 tttgccggct attccgcagc cttcaccgaa acagagcagg agcgcctccg atcggttttt
     3061 ccggacggtg tttgcgactg gtctaagccc ggactggaac aacagaagcc gctcggtacc
     3121 tggcttcgat actgaatttc cacctggata aacggacctg aatgcattag gattgggacc
     3181 atgcacaggt actggctggt ttgtggcctt gcggcgattc tttcgttcgc agccgaacgg
     3241 caggtagatc ccacctggct gcaccggcag gtgacggaag ttccagccgt tcgaac
//
LOCUS       DASTMK010000214         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_522805, whole genome shotgun sequence.
ACCESSION   DASTMK010000214 DASTMK010000000
VERSION     DASTMK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522805"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VFQ79_09535"
     CDS             complement(<1..1372)
                     /locus_tag="VFQ79_09535"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0139941.1"
                     /translation="MISHRVLAVLVLGAVAAVAQFTTANLGGTVADTSGGFVQGAKVT
                     ILNRDTGLTRTGETGADGTFSFPALPVGAYRLTVEREGFSTYVQEGITLTVNQVANQR
                     VTLQVGATTQQITVTENAAMVNTRTATIDQLISQRQVSDLPLNGRAAHALVLIAAGTS
                     ALPISGQGGIYGGRDQTFGGSQMAGVSGGGSDSVNYQMDGAGHNDTYVNMNLPFPNPD
                     AIQEFNLQSSSMSAAYGEGAAVVNIVTKSGTNAFHGNVFEFVRNGAMNARNYFAPTQD
                     TLKRNQFGATAGGPIKRDELFFFGTYQGTRIRSAPAGAVTYVPTAAERSGDFSSTAKQ
                     LVDPVTKTPFPGNQIPMSLFSGPSKYFLDKIPLPNGSGNQLTYLGPAAKPDDNHYMAK
                     IDWARGKQRLSGRYFYSKLNQQPDFSQVKQNIIAMDRNGNEVRVQTLALDHTYTASQT
                     LLFQTWF"
     gene            complement(1616..2938)
                     /locus_tag="VFQ79_09540"
     CDS             complement(1616..2938)
                     /locus_tag="VFQ79_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0139942.1"
                     /translation="MNRRYFLMSTAAVTTASRANPQRLPSDTVRVACVGFNGRGRAHI
                     QGYLKTPNTEIAALCDVDEAVLERGLKTVEAAGRKRPAAFTDLRKLLEDKSIDAISIA
                     TPNHQHTLQTIWGCQAGKDVYVEKPCSYNIFESRQIVAAARKYNRIVMHGTGASASLH
                     EARQAMADGAIGEVYMARGLCFKWRDTIGRTREEPVPPGVHYDLWLGPADKRPFTRNR
                     FHYNFHWFWDYGNGDFGNQGSHQMHAARWALGVKYPTKVSAAGGHFIFDDDQETPNTL
                     VVTLEYNHEGRKKMLIFEVRHWITNTEGEMAGKSDEMAGPAAGAPQTVGNLFYGSKGY
                     LVMRGGFRTFLGKEQAPGPASKQAERSSPFANFVAVVRSRKREDLKAEIEEGAISMVS
                     LHLGNIAYRLGRTLHFDPESMRCIGDDEANAMFTRKQYRPPFVIPKLV"
     gene            complement(2978..4177)
                     /locus_tag="VFQ79_09545"
     CDS             complement(2978..4177)
                     /locus_tag="VFQ79_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0139943.1"
                     /translation="MRTSQIFLVAVICGGAIVANSCGRKEPAEVKAAPSARESVRDAG
                     WLQARYGAWGGPGVRAAPGPMDAVELKDYAPKPSLIVPETSVVKARYPAIDVHAHVLA
                     KTPQEVADWVRTMDEVGIETTVVLTGATGPEFDKLAELYLKSHPTRFLLFCGMDRRDI
                     EKPDYPERVVAELVRCYQKGARGVGEISDKGWGFTRQNDLPRDKRLHPDDARLNAFWE
                     KCAELKMPVNLHVADHPSCWKPLDVYQERTPDYQHFNQSGKDVPSWEELIGARNRLLA
                     QHPRTTIIACHLGNQGHDLAALGKELDKYANLYLDISARDYEVGRAPKAAVKFLNRYR
                     SRVLFGTDMGRQKQMFTAWWRLLESGDEFMPGRIWWRYYGLELPAPTLESLYRGNARR
                     ILNWTKS"
     gene            complement(4200..5129)
                     /locus_tag="VFQ79_09550"
     CDS             complement(4200..5129)
                     /locus_tag="VFQ79_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEU0139944.1"
                     /translation="MLVGLCPLAVTLAAAQVRVTKLGDRVAISISGKPYADFFLAPDG
                     NKPYVYPLRTASGIIVTRRFPIEKVEGETTDHPHHRGMFFSHGDVNGLNFWATEPGSK
                     SARKASMVLKAVLEANSGEKSGTIKAVFEGLDPKGKPILRETRDLIFYSHPVLRIIDF
                     GIAIEALEKLVFGDTKEGTFGIRLATPLSEAKTGRMVNAEGRETEKNVWGKRSPWVDY
                     YGQLEGQTVGVAILDHPANPRHPTYWHSRAYGLFAANPFGIRDFTGDKTQDGSMTFEP
                     GQSVRFRYRVIIHPGDVKAANIAKAYKEYAASK"
     gene            complement(5484..>5664)
                     /locus_tag="VFQ79_09555"
     CDS             complement(5484..>5664)
                     /locus_tag="VFQ79_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139945.1"
                     /translation="FAVPGRERMRVQFRSEFFNLLNWVNLGNPNATLTSGTRMGRITS
                     AGSGRVIQFALKFLF"
BASE COUNT         1003 a         1809 c         1646 g         1206 t
ORIGIN      
        1 cgaaccacgt ctggaacagc agcgtctgcg aggccgtgta ggtgtgatcc aacgccagcg
       61 tctgcacacg cacctcgttg ccatttctgt ccatggcgat aatattctgc ttcacctgcg
      121 agaagtccgg ctgttggttg agtttcgaat agaagtaacg cccgctcagc cgctgttttc
      181 cgcgtgccca gtcgattttc gccatgtagt ggttatcgtc gggcttggcc gccgggccca
      241 agtacgtcaa ctgatttccc gaaccattcg gcaaggggat cttgtccagg aagtatttcg
      301 aaggcccgct gaacaggctc attgggatct ggtttccagg aaacggcgtc ttggtgaccg
      361 ggtccacgag ttgcttggcc gtgctggaga agtcgccgct gcgctccgcc gccgtcggga
      421 cgtaagtcac cgcccccgcg ggagccgacc ggatccgtgt gccctggtac gttccgaaga
      481 agaataactc gtccctcttg atcggtccgc cggccgtcgc accgaactga ttgcgcttca
      541 gagtgtcctg cgtgggagca aagtaattgc gcgcattcat ggcgccgttt cgcacaaact
      601 cgaacacatt cccgtgaaac gcgttggtcc ccgacttggt cacgatgttg accaccgccg
      661 ctccctctcc gtacgccgcc gacatgctgc tggattgaag attaaactcc tggatggcat
      721 ccggattggg gaacggcagg ttcatgttca cgtaggtgtc attatgcccg gcgccgtcca
      781 tctgataatt cacgctgtcg gagccgccgc cgctgacgcc cgccatttgc gagccgccga
      841 acgtctggtc ccggcctcca tagatgccgc cctggccgga aatcggcaga gcgctcgtgc
      901 ccgccgcgat caacaccagc gcgtgcgccg cccgcccgtt cagcggcaga tcgctcacct
      961 gtctctggct gatcagttgg tctatagtcg cagtccgggt attgaccatg gccgcgtttt
     1021 cggtgacggt gatctgctgc gttgtggcgc ccacctgcaa ggtcactctc tggttggcca
     1081 cctggttgac cgtcaacgta atgccctcct gcacgtaggt ggaaaagcct tcccgctcca
     1141 cggtcaaccg gtaggcgccg accggcagcg ccggaaatga aaacgttccg tcggcgcctg
     1201 tctcgcccgt gcgtgtcaat cctgtgtccc tgttgaggat cgtcaccttc gccccctgca
     1261 cgaaaccgcc gctggtgtcc gctacggtac ctcccagatt tgcagtcgtg aattgcgcca
     1321 ctgccgccac ggcccccaac accaggacgg ctaatacacg atgcgagatc attttcgttc
     1381 tcctttctcc taaatattgg gcgtacttac ttcacttcag ccacgcccgc cgaggctcgc
     1441 ggttgtggac tgcgagccta gaggtagggt aaacgccgcg caaacggtga ctgctaacgt
     1501 ccatcgggcg acggcgtcaa gcgctttttc ataaccagta cctccactta ccggttcctt
     1561 atactgctaa atgcccctat cctgacgggc gggtcatcgc gcccgccctt ctgactcaaa
     1621 ccagcttcgg tatcacaaac ggcggccggt actgcttcct cgtgaacatg gcgttggctt
     1681 cgtcgtcgcc gatgcagcgc attgattcgg ggtcgaagtg cagcgtccgg cccagccggt
     1741 aggcaatgtt ccccaagtgc agcgacacca tcgagatcgc accttcttcg atctccgcct
     1801 tcaagtcctc acgcttgcgg ctgcgcacca ctgcgacgaa attcgcaaag gggctgctgc
     1861 gttcggcctg cttcgacgcc ggcccgggcg cctgctcttt gccgaggaag gtccggaagc
     1921 ctccgcgcat gaccagatag cccttgctgc cgtaaaacag gttgccgact gtctgaggtg
     1981 cgcctgccgc cggtcccgcc atctcatccg acttgccggc catctcccct tcggtattgg
     2041 taatccagtg gcggacctcg aagatcagca tcttcttcct gccttcgtgg ttgtactcca
     2101 gtgtcaccac cagcgtgttc ggcgtctcct ggtcgtcgtc gaagatgaaa tgtccgcccg
     2161 cggcactaac cttggtcgga tacttcaccc ccaaagccca gcgggcggca tgcatctggt
     2221 gacttccctg atttccgaaa tctccattgc cgtagtccca gaaccagtgg aagttgtaat
     2281 ggaatcggtt ccgggtgaag gggcgcttgt ccgccggccc gagccagagg tcgtagtgaa
     2341 cgccgggggg cacaggctct tccctggtgc gcccgatggt atcgcgccac ttgaagcaca
     2401 gcccgcgagc catgtaaacc tcgccgatcg ctccgtcggc catcgcttgc cgggcctcgt
     2461 gcagggaagc cgatgcgccc gtgccgtgca tcacgatccg gttgtacttc cgtgcggcgg
     2521 cgacgatctg ccggctttcg aagatgttgt atgagcaagg tttctcgacg tacacgtcct
     2581 tgcccgcctg gcagccccag atggtctgga gggtgtgctg gtggttgggt gtggcgatcg
     2641 agatggcatc gatggatttg tcctcgagca gctttcgcag gtccgtgaag gcggcgggcc
     2701 gctttcggcc ggccgcttcc acggtcttca agccgcgctc cagcactgcc tcgtcaacgt
     2761 cgcacaatgc cgctatctcg gtattcggcg ttttcaggta cccctggatg tgggctcttc
     2821 cccgtccgtt gaaaccaacg caggcgactc gaaccgtgtc gctcggcagg cgctgcgggt
     2881 tcgcacggct cgccgtggtg accgcagccg tgctcatgag gaaatatctt cggttcatgt
     2941 tctgccctcc ccgtttgtag ccggctcacc cggcgattca actcttcgtc cagttcagaa
     3001 tccgtctggc gttgccgcga tacaggcttt ccagcgtggg agcgggaagc tcgagaccgt
     3061 agtaccgcca ccagatgcgt ccgggcatga attcatcccc actctccagg agccgccacc
     3121 aggccgtgaa catttgcttt tggcggccca tgtctgtgcc gaacaacaca cggcttctat
     3181 acctgttgag gaatttcacc gccgccttcg gcgcccgtcc cacttcgtaa tcacgcgcgg
     3241 agatgtcgag gtagaggttg gcatatttgt ccagttcttt gccaagcgca gccagatcgt
     3301 gtccctggtt gccgagatgg caggcgatga tggtggttct gggatgctgg gcgaggaggc
     3361 ggttccgggc gccaatcaac tcctcccaac tcggaacatc cttgccggac tggttgaaat
     3421 gctggtagtc gggagtgcgt tcctggtata catccagtgg cttccagcag gacgggtggt
     3481 ccgccacgtg caaattcacg ggcatcttca gctcagcgca cttctcccag aacgcattca
     3541 gcctggcatc gtccggatgt agccgcttgt cccgcggaag atcgttctgc cgggtaaagc
     3601 cccagccctt gtcgctgatt tcgccgacgc cacgcgcgcc cttctggtag cagcggacaa
     3661 gctcggcaac cacgcgctcc gggtagtccg gcttctcgat gtcgcgccgg tccatgccgc
     3721 agaaaagcag gaagcgggtg ggatgcgact tcaggtagag ctccgccagc ttgtcgaatt
     3781 ccgggccggt ggctccggtc agcaccacgg tcgtctcaat gccgacctcg tccatcgtac
     3841 gcacccaatc agcgacctcc tgcggcgttt tggccaacac atgtgcatgg acgtcgatgg
     3901 ccggatagcg cgccttcaca acggatgtct ccgggacaat caatgagggc ttgggcgcat
     3961 aatccttgag ttcgaccgca tccatcggcc cgggcgcagc ccgcaccccc ggaccgcccc
     4021 aggcaccgta cctggcctga agccatccgg catcacggac agattccctc gcagagggcg
     4081 cagctttcac ttcggccggc tccttccgac cgcacgagtt cgcgacgatc gcccctccgc
     4141 agattacggc caccaggaat atttggctgg tgcgcatgga attctccttc cggtcattgc
     4201 tatttcgagg cggcgtattc cttgtaagcc ttggcgatgt tggccgcttt gacatcgccg
     4261 gggtgaatga ttacgcgata gcggaagcga actgactggc ctggctcgaa agtcatgctg
     4321 ccgtcttgag tcttatcgcc ggtgaagtcc ctgatgccga agggattggc cgcgaaaagc
     4381 ccataggccc tggaatgcca ataggtgggg tgtctgggat tcgcgggatg gtccaggatg
     4441 gccacaccca ctgtctggcc ctccaactgt ccgtagtaat cgacccaggg tgagcgcttg
     4501 ccccaaacgt tcttctcggt ctcccggcct tcggcgttca ccattctgcc ggtcttggct
     4561 tctgaaagcg gcgttgccag ccggatgccg aaggtaccct ccttggtgtc gccgaatacc
     4621 agcttttcca gcgcctcgat ggcgatgccg aaatcgataa tgcgcagcac aggatgggaa
     4681 tagaaaatga ggtcgcgcgt ctctctcagg atgggtttgc ccttcggatc cagaccctcg
     4741 aacaccgcct tgatcgttcc cgacttctcc ccgctgttcg cctccagcac cgccttaaga
     4801 accatgctgg ccttcctggc gcttttggaa cccggctccg ttgcccagaa gttgaggccg
     4861 ttcacgtcgc cgtgagagaa gaacattccc cggtgatgcg gatggtcggt ggtctcgccc
     4921 tcgacctttt cgatggggaa ccggcgcgtg acgatgatgc ccgatgccgt tcgcagggga
     4981 taaacgtagg gcttgttccc atccggcgcc aggaaaaaat cggcataggg tttgccggag
     5041 atgctgatcg cgacccggtc ccccaacttc gtcacccgga cttgggcggc tgcaagcgtc
     5101 acagccagcg ggcacaaacc aactagcatc gtggcgcgaa aaactcggta tccggacaca
     5161 ctgaattctc ctcggtccct tatgctgcat ccggccgtcc gtacagtcaa cccgtcactc
     5221 gttacgtaac cgttacctga ggccgtggga ccgggcggaa gcccgcccag cgtcgagtca
     5281 gttcttctgc cacagacctc gacggtccgc tggaaaactg agtcgccgaa cgggcgggtg
     5341 ctgtccaaag ggaacagctt ccattttgcg tggcggcgcc atcactccag accacctcgg
     5401 caaagcggag cggaaatatt caccggacgt gtgcccgccc gcgcaagtcg aaaattcctg
     5461 gcggtgaggg tgcgtgacgc gatttaaaac aggaacttca gcgcgaactg gatcactcgc
     5521 cctgagcccg ccgacgtgat ccggcccatg cgcgtccctg aagtgagcgt cgcattggga
     5581 ttgccaaggt tcacccagtt caacaggttg aagaactccg agcggaactg gacccgcatg
     5641 cgctcgcgac ctggcacggc gaag
//
LOCUS       DASTMK010000215         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_106469, whole genome shotgun sequence.
ACCESSION   DASTMK010000215 DASTMK010000000
VERSION     DASTMK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106469"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..758)
                     /locus_tag="VFQ79_09560"
     CDS             complement(135..758)
                     /locus_tag="VFQ79_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0139946.1"
                     /translation="MQLDFEAAVLNVSLLWLLTAPHEFAHAWVATKLGDDTPAREGRV
                     TLHPLAHVDWLGTLILPAIISLTGAGFIGWGKPVNTNPARLRGGLNGLAVVALAGPAS
                     NVIFAIVLGLATVLFTRVLPVAAEYAAQGIYLSLYLAVFNMVPVPPLDGSKLLLAARI
                     PVAVYNELARFGFLLLIILVSSTGFGRWMSMVSLQGTERILGLFVSY"
     gene            813..2768
                     /locus_tag="VFQ79_09565"
     CDS             813..2768
                     /locus_tag="VFQ79_09565"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU0139947.1"
                     /translation="MFHRIELGIRDRVSSYLQARYGIGVPVSLEQPKQASFGELALPA
                     AFHLAKQLKQPPRAIAQQIAAEIGAIPGVASLEIAGNGYINIRLDRALYGSGILRGAA
                     DTQSEASDEKIIVEHTNINPNKAAHVGHIRNAVLGDTFVRMLRSAGSNVEVQNYIDNT
                     GVQVADVVVGFHHIEKLTSEDVRRLISGSSRPFDYVCWDVYARISAHYKEDPPSLAWR
                     QQVLHAIEANEGELAELAHLVADAIVERHLQTMLRLGIQYDVLPRESEILHLQFWARA
                     FELLKEGKAIYFETEGKNQGCWVMPGAAFSESAAEEDSKVIVRSNGTVTYVGKDIAYQ
                     LWKFGLLGQDFYYRRLLQYPDGHELWVTSDKPQGEDEPRFGRGSRVYNVIDSRQSYLQ
                     DVVAAGLRALGYTEQAERSIHFSYEMVALSPRTCAELGIELSAEDRNRPYVEVSGRKG
                     LGVKADDLIDKLIETALAEVDSRHPEEPEPARRAVATQIAVGALRYFMLKYTRNSVIA
                     FDFQEALSFEGETGPYVQYAAVRARNILRKLEERGGQLPDFAAMLPAEAMGRQLASED
                     LWQLLLSASKAGSAVERAIASGEPAHVAKYAFQLAQSFSNFYHEYPVIHEQDPEKRAF
                     LLWLTDYFRAQLERTLGILGIEVPPYM"
     gene            complement(2772..3182)
                     /locus_tag="VFQ79_09570"
     CDS             complement(2772..3182)
                     /locus_tag="VFQ79_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0139948.1"
                     /translation="MTCSDFENLILDEMDGCVSEPDRQLLASHLAVCDSCRCFQAEQL
                     SLDRTLSALASLDVPLHFTARVLQRTKAKPTPAQFLAWLDIVALAATAAAAVFAFHKL
                     FPNDTLGLSWVAAALVFSCGTWLAFEMEPVLDRF"
     gene            complement(3179..3724)
                     /locus_tag="VFQ79_09575"
     CDS             complement(3179..3724)
                     /locus_tag="VFQ79_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0139949.1"
                     /translation="MDGDIQAEIKAGHYREALELLLARYQRKVFRLAYSILGEEAAAE
                     EMAQDIFVKIWKVLPAYRGEASLSTWIYAITRNSCLTRRRQMRRHLTSSLDEAEVKAA
                     VEHGGQAAPERARTLDCAALLAELPPPYRRALALFYLENKSYDEVSAMLDLPAGTVKT
                     YLHRARKQLAASLAQAGRAGK"
     gene            3848..4636
                     /locus_tag="VFQ79_09580"
     CDS             3848..4636
                     /locus_tag="VFQ79_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139950.1"
                     /translation="MRRNACAVIRLAAASLAGASVLFASWIPYQISYQHRGSEPDYGN
                     KPEGHGKLLVLADLVRAVRPDGSQAEWETSYRLDGSVDYRHRTLILSNGIRLRGNETL
                     GLMTSIKNPKLPGVLARQALDPSKSCSVSYDGSRPSQNMLISQETLFGYETYKILIDM
                     KLARRTVWRAPALGCAELRDLFEKLDPQSGRVIAVGDRVATDTRAGEPDAALFEIPVG
                     FRNVSPSELVAAQSKNCCNRDLSNAQLSALAPRDEEFRKFRFDP"
BASE COUNT         1006 a         1374 c         1414 g          975 t
ORIGIN      
        1 tccttcttga aaagcttcac tgcctcaaag cgacgccgct ccaatgccac gaaatcgcgc
       61 ttcacgcctc gcgcattacc catgcgcgat tagacgctct gagtcctaga atgttccgct
      121 atattatgcg aaagtcagta ggacacgaag aggccgagaa tccgttcggt tccttgcagg
      181 ctcaccatcg acatccagcg gccgaacccg gtcgaggaaa ccaggatgat cagcaggaga
      241 aagccgaagc gcgccagttc gttgtatacg gcgacgggaa ttcgggcggc cagcaacagt
      301 ttcgatccgt cgagcggagg gaccggaacc atgttgaaca cggccaggta gaggctcagg
      361 taaattcctt gcgcggcgta ttccgcggct accggaagca cgcgtgtaaa cagcacggtc
      421 gccaacccca gcactatggc gaagatcacg ttacttgccg gacctgcgag cgccaccacg
      481 gccagtccgt tcaagccgcc gcgaagccgc gccggatttg tattcaccgg tttgccccat
      541 ccgatgaagc cggcacccgt caaggagatg atggccggta gaatcagtgt tcccaaccaa
      601 tcgacatgag caagagggtg aagggtgacc cggccttcgc gcgccggcgt gtcgtccccg
      661 agtttggttg caacccaggc gtgcgcaaac tcatgtggcg cggtgagcag ccatagcaag
      721 gatacattca atactgccgc ttcgaaatct aattgcattc ggtcttttca ttctgtcatg
      781 cggctgttcc cggctgctat actctttctc ttgtgtttca tcgaattgag cttggtatcc
      841 gcgatcgtgt ctcctcttac cttcaagccc gttacggcat cggtgtgcca gtatcgctgg
      901 agcagcccaa gcaggcttct ttcggcgagc tggcactgcc cgccgcgttc caccttgcca
      961 agcaactcaa gcagccgccg agagccatcg cgcagcagat tgccgctgaa ataggcgcga
     1021 tcccgggcgt cgcttcgctc gagattgcgg gcaacggcta catcaatatc cggctggacc
     1081 gggctctata tggctccggg attttgcgcg gcgccgctga cacgcaatcg gaagcatcgg
     1141 acgaaaagat tatcgtcgag cataccaaca tcaatcccaa caaagccgcg catgtcggcc
     1201 atatccgcaa cgccgtgctg ggggatacgt ttgttcgcat gctgcgctcg gccgggtcga
     1261 acgtcgaggt gcagaactac atcgacaaca cgggcgtaca ggtggcggat gtggtggtcg
     1321 ggtttcatca catcgagaag ctcacatccg aagacgtcag gcgcctgata tccggcagta
     1381 gcaggccttt cgattatgtc tgctgggatg tctacgcgcg catctccgcc cattacaagg
     1441 aggacccgcc ttcccttgcc tggaggcagc aggtgttgca tgccattgaa gcgaacgagg
     1501 gggagttggc cgagttggcg cacctggtgg ctgatgccat tgtggagcgg cacctccaga
     1561 ccatgctcag gctcgggatt caatatgatg tactgccgcg cgaaagcgag attctgcact
     1621 tgcagttctg ggccagggct ttcgagctgc tcaaggaagg taaggccatc tactttgaaa
     1681 cggaaggcaa gaaccagggc tgctgggtca tgccgggtgc cgcgttttct gaaagtgcag
     1741 cggaggaaga cagcaaagtc atcgtacgct cgaacggaac ggttacgtac gtcggtaaag
     1801 acatcgccta tcagttgtgg aaattcgggc tgcttgggca ggacttttat taccggagac
     1861 tcctgcagta tccggacggc catgaactct gggtgacctc cgataagccg cagggagaag
     1921 acgagccacg cttcggccgt ggttcacggg tctataacgt catcgattcc cggcagtcct
     1981 atcttcagga tgttgtggcc gccggcctgc gcgcgctcgg ctatacagag caggcagaac
     2041 gctcgattca tttctcgtat gaaatggttg cgctctctcc gcgcacctgc gccgaactgg
     2101 gcatcgaact ttccgcggaa gacaggaatc gcccgtacgt ggaggtgtcc ggccgcaaag
     2161 gtcttggcgt gaaggccgac gatctgatcg acaagctcat cgagaccgcg ctcgccgagg
     2221 tggactcccg ccatcccgaa gagccggagc cggcaaggcg ggcggtggca acgcagattg
     2281 cggtgggcgc gctccgctat ttcatgctga agtacacgcg gaactccgtc atcgctttcg
     2341 attttcagga ggcactgagt tttgaaggcg aaaccggccc gtacgtgcaa tacgcggccg
     2401 tcagggcgcg caacatcctg cgcaagctgg aagaacgggg cggccaattg cccgatttcg
     2461 cggccatgct tccggccgaa gccatgggcc ggcaactcgc ctccgaggac ctgtggcaat
     2521 tgctgctatc cgcttcgaag gcaggctcgg cggtggagcg ggccatcgca tcgggagagc
     2581 ccgcgcatgt cgcgaagtac gcgttccaac ttgcgcaatc attcagcaac ttctatcacg
     2641 aataccccgt gatacacgag caggaccccg aaaaacgggc cttcctgctc tggttgacgg
     2701 attacttccg ggcgcaactc gagcgcacgc tcggcatcct gggcatcgaa gttccccctt
     2761 acatgtaggg ctcagaaccg gtccaacact ggttccattt cgaaggccaa ccatgtgcca
     2821 cacgagaaaa ccagcgcggc cgcgacccag gacaacccga gagtgtcgtt cgggaacagc
     2881 ttatggaatg cgaaaacagc ggcggccgcg gtggcggcaa gggccactat atccaaccag
     2941 gccaggaatt gggccggagt cggctttgct ttcgtgcgtt ggagcacacg agcagtgaag
     3001 tgaagcggca catcgaggga ggccagcgcg gataatgtgc ggtccagtga caactgctcc
     3061 gcctgaaaac agcggcagga gtcgcacacg gccagatggc ttgccaggag ctgccggtcc
     3121 ggctccgata cgcagccgtc catctcatcg agtatcaggt tttcaaagtc ggaacatgtc
     3181 attttcctgc cctccctgcc tgggcgagag aagccgccaa ttgtttacgc gcccggtgta
     3241 agtaggtctt cacggtaccc gcaggcagat cgagcatcgc ggatacttcg tcgtaggatt
     3301 tgttttccag atagaagagt gccagtgcgc ggcggtaggg cggcggcagc tccgccagaa
     3361 gcgcggcgca atcgagcgtt cgggcgcgtt ccggggcagc ctgcccgccg tgctcgacgg
     3421 ctgccttcac ttcggcctcg tcgagcgaag aagtaaggtg ccgccgcatc tgccggcgcc
     3481 gtgtcaggca gctattccgc gtgatcgcat agatccaggt agaaagcgac gcttcgccgc
     3541 gatacgccgg aagaactttc cagatcttca caaatatgtc ctgcgccatc tcttccgctg
     3601 ctgcttcctc gcccagaatg gagtaggcca gacggaagac tttgcgctgg taacgcgcca
     3661 gcaaaagctc gagcgcttcc cggtaatgcc cggccttgat ctccgcctgt atgtcgccgt
     3721 ccatcggcgc gttccacgcc ttgccgtact tatcttacga ctgcttccca tgccgggaaa
     3781 gtttcaaaag atgccccacg ccattgcggt gtttgacgtc agggatattg ttgatggagt
     3841 gtaatcgatg agacgaaatg cgtgtgccgt cattcgcctg gctgcggcat cgctggctgg
     3901 cgccagtgtg ttgttcgcaa gctggatacc ctatcaaata agctatcagc atcgcggcag
     3961 cgagccggat tacggtaaca agccggaagg acacgggaaa ctgctggtat tagccgacct
     4021 cgttcgtgct gtccggccgg acggttctca ggcagaatgg gagacttcct accggctcga
     4081 cggctcggtc gactatcgcc atcgcacact gatactctcg aacggcattc gcctgcgagg
     4141 taatgagacg ctcgggctta tgacttcgat caagaacccc aaattgcccg gggtgcttgc
     4201 ccggcaggcg cttgatccat cgaagtcatg cagcgtttcc tatgatggca gccgcccgag
     4261 tcagaatatg ctcatctctc aggaaacgct gtttggatat gaaacttata agattcttat
     4321 cgacatgaag ctggctcgac gcacggtgtg gcgtgccccc gcgctgggtt gtgcggaact
     4381 gcgtgatctg tttgaaaagc tggacccaca atcaggccgg gttattgccg tcggggacag
     4441 ggtagctacg gacactcggg cgggcgaacc ggatgcggcg cttttcgaaa tacctgtggg
     4501 ttttcgaaac gtgtctccaa gcgagctggt agcagcccaa tcgaaaaatt gctgtaatcg
     4561 agacctctcg aacgctcaac tttcggccct tgcccccagg gatgaggaat tccggaaatt
     4621 ccgcttcgac ccataagatt tgaaacacag gaacaggttc agaaagtctc aacttatgtt
     4681 ggaggcggtg tttgatgaac gaacaaattg ttcctgtcac tgcaatcgta atgtccatgc
     4741 tggtcgtggc ggtggcaatc tatgcgggt
//
LOCUS       DASTMK010000216         9955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_362269, whole genome shotgun sequence.
ACCESSION   DASTMK010000216 DASTMK010000000
VERSION     DASTMK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362269"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFQ79_09585"
     CDS             <1..424
                     /locus_tag="VFQ79_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139951.1"
                     /translation="PALLVERQKRLSGPATTEELILYDHVLDYRDGRLSERPSLSKIP
                     GPTVPLVFVPEKVHPGDSYIVRAPALAGKTVDVAVRLQTGSVIQAAIVRNWCTLDASG
                     EAKLVTPADHLSATVQIVGIRQHNGPWLQAKGELAVVR"
     gene            complement(433..1476)
                     /locus_tag="VFQ79_09590"
     CDS             complement(433..1476)
                     /locus_tag="VFQ79_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEU0139952.1"
                     /translation="MATQRRLIAPPGPKPVGPYSPGILAGDVLYVSGQGVRRPDGTVP
                     ATPKAQAEQCLANVENIVKAAGMSMADVVYSQIYLIDIATRNLVPMANPHSVLGMARM
                     PTETTFEINAIATKSQAARDSTIYIPGMFGGDPKEAIGKVQEFLKTAGADLSHAVFLN
                     VYLSEGATLEDFDAVYRQYVEPGYSPARAALAVNALPEKAAVEITGVAVRDLRQRRVI
                     RPKNMSAKALESPAVLAGDTLYCSLRSAAIPGPNGGVFSDNVQLQARLTMRNLLDSLE
                     EAGLDFSHVVSSNVYVDNMDEFASMNSIYGSYFSSMPPTRTTVQPVRPAGRVAANGGQ
                     FPPLVRISMVAVR"
     gene            complement(1479..2600)
                     /locus_tag="VFQ79_09595"
     CDS             complement(1479..2600)
                     /locus_tag="VFQ79_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0139953.1"
                     /translation="MLSRRSFTRAIAAGWLSGTAGLWSEAALAQRALVEGELPEDMVW
                     LNANENPEGPPQVSLAAMQEVMPTAWRYHYQEFRDFYRRVAGSVGLSSGEVYVGQGSS
                     EVLQVAVVAFGSASHPLITMEPTFELPVGVARAFGIPVVRVPLTNGYYADVKKLAEEA
                     GKAGGGLLYICNPNNPTAAVTPKTDIAWLVSNLPANTILLVDEAYIHFAETPEIESAL
                     RYVREGKDVIVARTFSKIYGMAGLRVGFSCARQELMGRMWPYRSGVISIVSARAVMAA
                     LNDAQAIEDGRRKRFAAVRNELCAWLRGKGLEYIEPHANFLMIDVGRDVREFSPQMAR
                     LGVAVGRPFPPLTNMLRVSIGAESDMARFRSAFERVYKG"
     gene            complement(2825..3430)
                     /gene="frr"
                     /locus_tag="VFQ79_09600"
     CDS             complement(2825..3430)
                     /gene="frr"
                     /locus_tag="VFQ79_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU0139954.1"
                     /translation="MKPAQPGTTGGSTFRSVKEVDAHSRARMEKVLTDMQQEMATIRT
                     GRASLSLLDMIRVDYYGTPTPLNQVGTLHVPEPSLITVQPWDPSLIGPIEKSIRSSDL
                     GLNPSNDGKLVRIPIPPLTEERRKELVKRLHHITEERRVAMRNIRRDANEAVKKMVKE
                     KLISEDDERRAHDEIQKLTDAHIEKLDQAAKVKEKEILEIK"
     gene            complement(3504..4286)
                     /locus_tag="VFQ79_09605"
     CDS             complement(3504..4286)
                     /locus_tag="VFQ79_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139955.1"
                     /translation="MLESLDLSRSLDRRTYVNELTRRQIQLRELGYQVYLQKRPVVIL
                     FEGWDAAGKGGAIKRLTEKMDPRGYAVYPISAPEGEDRTHHYLYRFWRRLPERGQIAI
                     FDRSWYGRVLVERVEELSGEAAWKRAFREINSFERQLTGFETILLKFWIHISRDEQLR
                     RFEDRRKSGYKAWKLTSEDWRNREKWGSYTTAVEEMLLRTSTAKAPWTLVEGNSKWFA
                     RVKTLTTVVETLSRELDYRPSEPLAKHKNKNSKEASGNRKII"
     gene            complement(4286..5089)
                     /locus_tag="VFQ79_09610"
     CDS             complement(4286..5089)
                     /locus_tag="VFQ79_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEU0139956.1"
                     /translation="MLEKLDLDLELSHHEYKLQLPGWQRRLYDLEKACWDNKIGSVVV
                     FEGWDASGKGSTISTLTARLDPRGFKLHSIQAPRTFEKNYPWLWRFWQRIPNYGEMAI
                     FDRSWYGRVLVERVEKLFPESEWRDAYRDIVEFERMLPDDGIAIVKFWLHISKKEQKR
                     RFKELEDDPLESWRVTKEDWKRHRKYKRYLEAAEEMIATTDSEYAPWTIVEATCWWYA
                     RSKVFKTLIAALEARLGAAAPATRADTSGADNELREAMERAETATAQEA"
     gene            complement(5202..5978)
                     /gene="pyrH"
                     /locus_tag="VFQ79_09615"
     CDS             complement(5202..5978)
                     /gene="pyrH"
                     /locus_tag="VFQ79_09615"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0139957.1"
                     /translation="MQASAPVPDSPGERRPATGKYERILLKLSGEVMAGQSSFGVDPE
                     RVRALAAEISEVAAAGVQLGLVVGGGNFFRGVSAAAQHMDRVTADHMGMLATVINALA
                     LQDALEKKGVPTRVMTAIEMRQVAEPYIRRRAIRHLEKGRIVIFAAGTSNPYFSTDTA
                     ATLRALEIRAQVIAKATRVDGVYDKDPLKYPDARFYHEISYLEVLAQGLKVMDASAVA
                     MCRDNQLPILVFNLNTPGNIMRVATGEPAGTIIRDSTVAH"
     gene            complement(5978..6667)
                     /gene="tsf"
                     /locus_tag="VFQ79_09620"
     CDS             complement(5978..6667)
                     /gene="tsf"
                     /locus_tag="VFQ79_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU0139958.1"
                     /translation="MAEITAQMVKDLRERTGAGMMECKAALVEAKGNIEEAVTILRKK
                     GIASAAKKAARTTKAGVIGTYIHQGSQLGVLIEVNCESDFVARTEDFQQLVHDLAMQV
                     AAADPKFVRKEDVTPEALEKEKEIHRARALNEGKPEKMLERIIEGRLAKFYEEVCLYE
                     QPYIKDNGVTIKDLIASKIATIKENITVSRFVRFKVGETASDAGSGETGSAEPVPVAG
                     TPAPKESTSAQ"
     gene            complement(6754..7659)
                     /gene="rpsB"
                     /locus_tag="VFQ79_09625"
     CDS             complement(6754..7659)
                     /gene="rpsB"
                     /locus_tag="VFQ79_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0139959.1"
                     /translation="MPSISMKELLEAGVHFGHQTKRWNPKMKEYIFGERNGIYIIDLQ
                     KTLKLFKEAMRFVGEMAAQGKSVLFVGTKRQAQEAVAEEATRCEQFYVNQRWLGGLLT
                     NMMTVQRSIKRLKELESMANETGYAGRAKKEIGRLERERKHLDSNLAGIKNMNGLPDL
                     LFVIDSNKEAIAVKEARKLGIPVVAVVDTNCDPDEVDYVIPGNDDALRAIRLFTNKIS
                     DAVIEGRALATEHEFAAEKIISDEVETGLDEIPEHTQYFDPQYVEQLMSEGISDEDLP
                     SVSLPRKGPGQQEAPEQVSGNVNEQ"
     gene            complement(7809..8207)
                     /gene="rpsI"
                     /locus_tag="VFQ79_09630"
     CDS             complement(7809..8207)
                     /gene="rpsI"
                     /locus_tag="VFQ79_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU0139960.1"
                     /translation="MAATLVQYLGTGRRKSAIARIFLRPGKGQISINGRAFDDYFKTE
                     ASRAVVRQPLLATETSDKFDVLILTEGGGQLGQAGAVRLGIARALVEFNSELRGKLKQ
                     LGFLTRDPRAHERKKYGQRGARRRFQFSKR"
     gene            complement(8225..8659)
                     /gene="rplM"
                     /locus_tag="VFQ79_09635"
     CDS             complement(8225..8659)
                     /gene="rplM"
                     /locus_tag="VFQ79_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU0139961.1"
                     /translation="MSTQFPSKSSIERKWLVIDADGQVLGRVASRAAKVLMGKHKPIY
                     TPFLDTGDHVVVVNAEKVRLTGHKEEDKLYRRHSGYPGGLTEINARKMRSTRPVRMLE
                     LAISGMLPKTKLGKQMYRKLKVYAGDRHPHQAQKPVALEPGK"
     gene            complement(8794..>9955)
                     /locus_tag="VFQ79_09640"
     CDS             complement(8794..>9955)
                     /locus_tag="VFQ79_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0139962.1"
                     /translation="GGLVIDLAAMRGTRVDPVSHTVRAQAGCTWGDVDHATHAFGLAT
                     VSGIISTTGIGGLTLGGGHGHLTRKYGLTIDNVLSVDIVLANGRLVTASANENQDLFW
                     AIRGGGGNFGVATSFLYRLHEVKNIVGGPTLYPIEQAREVLRWYRDFIPQAPLELNGF
                     FAFLVVPPAPPFPEHLHLRNMCGIVWCYAGRENEAERIFKPIREFGPPALYGVHVMPY
                     PALQSAFDALYPPRLQWYWRGDFINEIPDAAIDVHLKYGSQLPTMHSTMHLYPIDGAS
                     HRVGRNETAFSYRESRWSQIIAGVDPDPTNADRITGWVRNYFDELHPYSAGGAYVNFM
                     MEEGQERVRATYRENYGRLSEIKSKYDPQDLFCVNQNIRPLPDERHQPVQHK"
BASE COUNT         1895 a         2986 c         2838 g         2236 t
ORIGIN      
        1 tccggcgctg ctggtcgagc ggcaaaagcg gctcagcgga ccggccacca cggaagaact
       61 cattctctac gatcacgttc tggactaccg ggacgggcgt ctttcagaga gaccttcgct
      121 gagtaaaata cccggcccta ccgtgccatt ggtctttgtg cctgaaaaag tgcaccccgg
      181 tgatagttac attgtgcgtg cacccgcgct ggcgggtaaa acggtggatg tagcggtccg
      241 cttacaaacc ggctccgtga tccaggctgc aattgtgcgt aactggtgca cgcttgatgc
      301 gagcggcgaa gcgaagctgg ttactccggc agaccacctt tccgccaccg tgcagatcgt
      361 cggaatccgg cagcataacg gtccttggct ccaggcgaag ggcgagcttg ccgttgtccg
      421 ctgaggcggc tgttaccgta ccgctaccat ggaaatccgc acgaggggtg ggaactgccc
      481 gccgtttgcc gcaacacggc cggcgggcct caccggctgt accgtggtgc gcgtgggcgg
      541 catggaagaa aaatagcttc cgtaaatgct gttcatgctg gcaaattcgt ccatgttatc
      601 aacgtacacg ttgctggaaa caacgtgcga aaaatcgagc ccggcttcct ctaagctgtc
      661 cagcagattg cgcattgtaa ggcgcgcctg aagttgcaca ttgtcgctga agactccacc
      721 gttagggccg ggaatggcgg cgctgcgaag cgaacagtac aaggtgtcgc ccgcgagaac
      781 cgcgggactt tccagggctt tagcagacat gttcttcgga cggatgaccc gccgttgccg
      841 caaatcgcgc acggctactc cggtaatttc gaccgccgcc ttttccggca gagcgtttac
      901 ggccagggcc gcgcgcgccg gagaatatcc gggctccaca tactggcggt acacggcatc
      961 gaaatcctcg agcgttgccc cttccgagag atagacattc aggaacacgg catggctgag
     1021 gtcggcgccg gcggtcttaa ggaactcctg cacctttccg atggcttcct tgggatcgcc
     1081 gccaaacatt ccggggatat agatcgtgct gtcccgggcg gcctggcttt tcgtggcaat
     1141 cgcattaatc tcgaacgtcg tctccgtcgg catgcgcgcc attccgagaa cggagtgggg
     1201 gtttgccatg gggaccaggt tccgtgtagc gatgtcaatc aggtagatct gggaatacac
     1261 gacatccgcc atggacatgc ctgccgcttt cacgatgttt tctacattgg cgagacactg
     1321 ttcggcctgc gctttcggag ttgccggcac ggttccatcg ggccgccgca ctccctgccc
     1381 agacacgtag agaacatcgc cggcgagaat gccagggctg taaggcccca ccggcttcgg
     1441 gccgggaggg gcgatgagac gcctttgagt agccattctt atcctttgta cacgcgttcg
     1501 aaggcgcttc ggaagcgcgc catgtcggac tcagcgccga tggaaacgcg aagcatattg
     1561 gtgaggggag ggaaggggcg gcctaccgct acaccgagcc gcgccatttg cggcgagaac
     1621 tcgcgcacgt cgcggcccac gtcgatcatc agaaaattgg cgtgcggttc gatgtattcg
     1681 aggcccttcc cacgcagcca ggcacagagt tcgttgcgca cagcggcgaa ccgtttgcgg
     1741 cggccatcct caatcgcctg cgcatcgttc aacgcagcca tcacggcacg cgcgctgacg
     1801 atggagatca cgccggaacg gtacggccac attctgccca tcagttcctg gcgtgcgcaa
     1861 ctaaacccga cacgcagccc cgccataccg tagatcttcg aaaacgtgcg cgccacgatc
     1921 acatctttcc cttcgcgcac gtaacgtaga gcgctctcta tctccggcgt ctccgcgaaa
     1981 tggatgtaag cttcatcgac gagcaggatg gtattggcag ggaggttcga aaccagccac
     2041 gcgatatcgg tttttggcgt tactgcggcg gtagggttat tgggattgca gatgtagagg
     2101 aggccgccgc ccgccttccc ggcttcttcc gcgagttttt tcacgtccgc atagtagcca
     2161 ttggtcagcg ggacgcgaac gaccgggatc ccgaaagcgc gggccactcc tacgggcaat
     2221 tcgaacgtag gttccatggt gatcagaggg tgggaagcgg acccgaacgc gacaacggcc
     2281 acttgcagca cttcgctaga cccttgcccc acgtacactt cgccggagga gaggccgaca
     2341 ctcccggcca cgcgccggta gaaatcccga aactcctggt agtggtagcg ccacgcagtc
     2401 ggcatgacct cctgcatggc agcgagggag acctgcggag gaccttccgg gttctcattc
     2461 gcgttcagcc agaccatgtc ttccggcaac tcgccttcca ccagggcgcg ctgcgcaagc
     2521 gccgcttcac tccataggcc ggcagtcccg gaaagccacc cggcagcaat cgcgcgggtg
     2581 aaactgcgac gactcagcat ctcccccacc tcctccgtac ccgtcattcg ccgaccgctc
     2641 gctcagaagc tgtgaagaaa ttgaatttag ccacggatga acacggatga acacagataa
     2701 gtttcctgtt ttcaatctgt gtttatctgt gtccatctgt ggctctgaat ctttcacacc
     2761 ttctcacgct cgcggctctg cctgagccac gaccgtcagg gagtggtcaa ccgggggcca
     2821 cggattactt gatttccagt atctccttct ccttcacctt cgctgcctga tcgagtttct
     2881 caatgtgggc atcggtcagc ttctgtatct catcatgcgc ccggcgttcg tcgtcttcgc
     2941 tgatcagctt ctccttcacc atcttcttga ccgcttcgtt cgcgtcgcgg cgaatattgc
     3001 gcatcgccac acgccgctct tctgtaatgt ggtgaagccg ctttaccagc tctttgcgcc
     3061 tttcctcggt cagaggagga atcgggatgc gcaccagttt gccatcgttc gatggattca
     3121 atcccaggtc cgagctgcga atcgacttct cgatcggacc gatcagcgaa ggatcccagg
     3181 gctgcacggt gatcagcgag ggctccggca cgtgtagtgt tcccacctgg tttaacggcg
     3241 ttggggtgcc gtagtaatct acgcgaatca tatccagcag gcttaaagag gcgcggccgg
     3301 tgcgaatggt agccatttcc tgctgcatat cggtaagcac cttttccatg cgcgctctag
     3361 aatgtgcgtc cacctccttc acggatcgga acgtactgcc gccggttgtc cccggctgtg
     3421 ctggtttcat gatgctctcg ctccaacgga tgtatttgat gatgtcaaac tatgtaaggg
     3481 caggttggcc cgccacctag agattatatg attttgcggt tgcccgatgc ctctttcgag
     3541 ttcttgttct tatgcttcgc gagcggctcg gacgggcggt agtcgagctc acgggacaaa
     3601 gtttccacca cggtggtaag cgtcttcacc cgcgcaaacc acttactgtt accctccacc
     3661 agcgtccagg gcgctttggc tgtgctggtc cggagcagca tctcttccac cgcggtggtg
     3721 tagctgcccc atttctcacg atttctccag tcctcactgg tgagcttcca ggccttgtag
     3781 cctgatttgc ggcgatcctc aaaccggcgc aattgttcat cgcggctgat atggatccag
     3841 aatttaagca ggatggtctc gaagccggtg agttgccgct cgaaggagtt gatctcccgg
     3901 aaggcccgtt tccaggcggc ctcgcctgag agttcctcca cgcgctccac cagcacgcgt
     3961 ccataccagc ttcggtcgaa tatagcgatc tgtccccgtt cgggcagccg ccgccagaag
     4021 cggtagagat agtggtgtgt cctgtcttcg ccctcgggag cgctgatcgg gtagacggca
     4081 tagccgcgcg ggtccatctt ctcggtcagc cgtttaattg ctccgccttt gcccgccgca
     4141 tcccaccctt cgaaaagaat gacgacggga cgtttttgca ggtatacctg atagcccagc
     4201 tctcgaagtt ggatctgccg cctggttaac tcgtttacgt aggtccggcg gtcgagagat
     4261 cgggaaagat ccagcgattc gagcatcacg cctcctgcgc cgtggcggtt tcggcacgtt
     4321 ccatcgcttc ccgcagttcg ttgtcggcgc ccgaagtatc ggcgcgtgta gcaggcgccg
     4381 cggcgcccag ccgggcttcg agcgcggcga tcaacgtttt gaataccttg ctgcgtgcat
     4441 accaccagca ggtggcttcg acaatcgtcc acggcgcata ttcggaatcg gtggtagcga
     4501 tcatctcctc ggcggcttcg agatagcgct tgtacttccg gtgccgcttc cagtcctcct
     4561 tggtgacacg ccagctttcg agcggatcgt cctcgagttc tttgaaacgc cgcttctgct
     4621 cctttttgct gatatgcaac cagaacttga cgatggcgat gccgtcgtcg ggcagcatcc
     4681 gctcgaactc cacgatatcc cggtacgcgt cgcgccactc ggattccggg aagagctttt
     4741 ctacccgttc gacgagtacc cgtccgtacc agctccgatc gaagatggcc atttcgccgt
     4801 aattgggaat acgttgccag aatcgccaca gccaagggta attcttttcg aaagtgcggg
     4861 gcgcctggat ggagtgcagc ttgaagccgc gggggtcgag ccgggcagtg agcgtggaga
     4921 tggtcgagcc cttcccggag gcatcccatc cttcgaatac gacgacgctg ccgattttgt
     4981 tatcccagca cgctttctcc aggtcgtaga ggcggcgctg ccagccgggc agttgcagct
     5041 tgtattcgtg gtgggataac tccaggtcga gatccagctt ttcgagcatg ttccctccgt
     5101 tgccaaccac tcacttacgt tcgtggctca gaagcgatta cggagccgcg agcgttagcg
     5161 agcggtcttg gcgaactgac acggttattt cctgacggag actaatgcgc tacggtgctg
     5221 tcgcgaataa tggtgccggc cggctcgccc gtggccacac gcatgatgtt gcctggcgtg
     5281 ttcaggttga aaacaagaat cggcaactga ttgtcacggc acatcgcgac cgccgatgca
     5341 tccatcactt ttagcccctg cgcaagcact tccagataag aaatctcatg atagaaccgg
     5401 gcgtcgggat acttcagtgg atctttgtcg tacactccgt cgacacgcgt ggccttggca
     5461 attacctgtg cgcgaatttc gagcgcccgg agcgttgcgg ccgtgtcggt ggagaagtac
     5521 gggttggagg tgccggcggc aaagatgacg atgcggccct tttccagatg acgtatggcg
     5581 cggcggcgaa tgtagggctc ggccacctgt ctcatctcga tggccgtcat gacgcgcgtg
     5641 ggcacgcctt tcttttcgag tgcatcctgc aaggccagcg cattgatgac ggtcgccagc
     5701 atgcccatgt gatccgcggt gacgcggtcc atatgctgag cggcagccga cacgccgcga
     5761 aagaaattgc cgccgcccac aaccagtccc agttgcactc cggcggcggc tacttcggaa
     5821 atttccgcag ccagggcgcg aacacgttcg gggtcgacgc caaaggacga ctggcccgcc
     5881 atcacttcgc cgctcagctt gagcagaata cgctcgtact tgcccgtggc gggccgcctc
     5941 tcacccggag agtccggcac tggagcggag gcctgcatta ctgggccgaa gtagactctt
     6001 taggcgcggg cgtcccggcc accggtaccg gttcggcgga gccggtttct ccggagcccg
     6061 catccgatgc ggtttccccg accttgaacc gcacaaagcg cgaaacggtg atgttttctt
     6121 tgatggtggc aatcttggag gcgatcaggt cctttatggt gacgccatta tccttgatat
     6181 acggctgttc gtagaggcag acctcctcgt aaaatttcgc cagccttcct tcgatgatgc
     6241 gctccagcat cttctccggc ttgccttcgt tcaacgcccg ggccctgtga atctccttct
     6301 ccttttccag tgcctcggga gtaacgtcct ctttgcggac aaacttgggg tccgccgccg
     6361 ccacttgcat ggcgagatcg tgaacaagct gctggaaatc ctccgtgcgg gcgacgaaat
     6421 cggattcgca gttcacttca atcaggacac cgagctgaga accctggtgg atataggttc
     6481 cgatcacgcc cgccttggtc gtcctcgcgg ctttcttggc ggccgatgcg atacccttct
     6541 tgcgaaggat ggtgacggct tcttctatgt tgcctttcgc ctccaccaaa gcggctttgc
     6601 actccatcat gcccgctccc gtgcgctcgc ggagatcctt aaccatttgc gcggtaattt
     6661 cagccatatc taccagtcct atattttctg acattcaaag ccggcagccg cggggccgga
     6721 acggaccggt cccgcggcct gcggcagaca agtctactgt tcgttgacgt tgcccgaaac
     6781 ctgctccggg gcttcctgct gaccgggccc tttccgcggc agcgataccg agggaagatc
     6841 ttcgtccgag atcccctcgg acatgagttg ctcgacatac tgcggatcga aatactgggt
     6901 gtgctccgga atctcatcga gaccggtttc cacttcgtcg gagatgatct tctccgccgc
     6961 aaattcgtgt tcggtggcca gggcgcggcc ttcgatcacg gcatcggaga tcttgtttgt
     7021 gaacaggcgg atggcgcgaa gcgcgtcatc gttgcccggg atgacgtagt ctacttcgtc
     7081 gggatcgcaa ttcgtgtcca ccacggcgac cactggaata ccgagcttgc gtgcttcctt
     7141 caccgcgata gcttccttgt tggaatcgat gacgaagagc aggtcgggca gtccgttcat
     7201 gttcttgatg ccggcgaggt tcgaatccag gtgtttacgc tcgcgctcga gccgtccgat
     7261 ttccttcttc gcgcggccgg cgtatcctgt ctcgttcgcc atcgactcga gctctttcag
     7321 ccgcttgatg gaacgctgca cggtcatcat gttcgtcagc agtccgccca gccagcgctg
     7381 gttaacgtag aactgctcgc agcgcgtggc ttcttccgcg acggcctcct gcgcctggcg
     7441 cttggtgccg acaaatagaa ccgatttgcc ttgcgcggcc atctcaccga cgaagcgcat
     7501 ggcttccttg aatagcttaa gggtcttctg gagatcgata atgtagatcc cgttacgttc
     7561 gccgaagata tactctttca tcttcggatt ccagcgcttg gtctggtgcc cgaagtgaac
     7621 gccggcttcg agcaattctt tcatcgaaat tgacggcaaa atgcctcctg tttcttatcg
     7681 gggatcgttc ccgaagggtt caaactacac cctggtacgc gatccaaagc gagatttgtg
     7741 taccgggtga aaaacggggc ggaaagagcg ggttggatac attgttccaa cccgcggcac
     7801 tgcattggtt accgcttgga gaactggaac cggcggcggg caccgcgttg tccatacttc
     7861 ttacgctcgt gggcgcgcgg atcccgggta aggaatccaa gttgcttcag tttgccgcgc
     7921 aactcggagt tgaattccac cagtgcgcgt gcgatcccta accggaccgc gccggcctga
     7981 cccaactgac ctccgccttc cgtcaggatg agaacatcga acttgtctga tgtctctgtt
     8041 gcaagcaggg gctgccgcac aaccgcgcgg gaagcctccg tcttgaaata atcgtcgaat
     8101 gcccgcccgt taatactgat ctgacccttc ccgggcctca gaaaaatacg ggcgatcgcg
     8161 cttttacgtc tgcctgttcc caggtactga actaacgtcg ctgccactca attcctctcg
     8221 taaactattt gcccggctcg agggccactg gtttctgtgc ctgatgcgga tgacggtcgc
     8281 ccgcatatac ctttagtttg cggtacatct gcttgcccag tttggttttg gggagcatac
     8341 cggaaatcgc gagttccaac attctcaccg ggcgcgtgga ccgcattttc cgcgcgttga
     8401 tttcggtcaa tccgccggga tatcccgagt ggcggcggta aagcttgtct tcttctttat
     8461 ggccggtgag ccgcactttc tcggcgttta ctactaccac gtgatctccg gtatcgagga
     8521 aaggcgtgta gatcggcttg tgctttccca tcaaaacctt cgcggcacgg cttgcaacac
     8581 ggccgaggac ctgcccatcg gcgtctatta ccagccattt gcgctcaata ctgctcttgg
     8641 acggaaactg ggtgctcatt agacagaggt actccctgcg gaactcacaa actattaagc
     8701 ttaccgaagc ggacgtttgg agtcaaattg aacttgcgca cggtggccag gctcttgccg
     8761 gccattgggt gggcactgcc gatttccact tgtctattta tgctggacgg gctggtgccg
     8821 ctcgtccggc agcggcctaa tgttctgatt cacgcaaaac aagtcttgcg gatcgtattt
     8881 cgacttgatc tccgaaagcc gcccgtagtt ctcccggtat gtggcccgga cgcgttcctg
     8941 tccttcttcc atcataaaat tgacgtaagc gccgcctgcc gaatacggat gtaactcgtc
     9001 gaagtagttc ctgacccaac ccgtgatgcg atctgcgttc gtaggatccg gatcgacgcc
     9061 ggcaattatc tgggaccacc tggattcccg gtagctaaaa gccgtttcat ttctaccaac
     9121 ccggtgagac gctccgtcga tcgggtagag gtgcatggtg gaatgcatcg tgggcaattg
     9181 cgagccatac ttcagatgca cgtcgatagc agcatccgga atttcattga tgaaatcgcc
     9241 tctccagtac cactgaaggc gcggcggata caacgcgtca aatgcacttt ggagagccgg
     9301 atacggcatg acatgtacgc cataaagggc cggagggccg aactctcgaa tcggtttgaa
     9361 tatccgttcc gcttcattct cgcgacctgc gtaacaccat acaattccgc acatgtttct
     9421 gagatggaga tgctcgggaa atggcggtgc gggcggcaca acgaggaagg cgaagaagcc
     9481 gttcaattcc aagggagcct gcggtatgaa atccctatac caacgcagaa cctccctggc
     9541 ctgctcgatc ggatagagcg tcggaccgcc aacgatattc tttacctcat gcagcctgta
     9601 caggaaagat gtggcgacgc cgaagtttcc tcccccgccg cggatcgccc agaacaaatc
     9661 ctggttttcg ttggcgctgg ccgttaccag gcgcccgttg gcgagaacga tgtcgacgga
     9721 aaggacgttg tctatcgtca ggccgtattt gcgagtaaga tgaccgtggc ccccacccaa
     9781 ggtgaggcct cctataccgg ttgtcgagat aatgccgcta accgtggcca agccgaaagc
     9841 atgagtcgca tgatctacat cgccccaggt gcagcctgct tgtgcccgga ccgtgtggct
     9901 caccggatcc actcgcgtgc cacgcatggc cgcgaggtcg atcaccaagc cacct
//
LOCUS       DASTMK010000217        23165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_586557, whole genome shotgun sequence.
ACCESSION   DASTMK010000217 DASTMK010000000
VERSION     DASTMK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 23165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23165
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586557"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFQ79_09645"
     CDS             complement(<1..347)
                     /locus_tag="VFQ79_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139963.1"
                     /translation="MRKILLLLTLLVVSGAIVWIWSRRSAPPEVPFAPVTRSTLVDML
                     ATNGKADPAEWTAVHAQKAGPVQRVLVQQGQVVVKGAALVELESIDAVTDLAAAEARA
                     RQASAELEIRQKGG"
     gene            558..764
                     /locus_tag="VFQ79_09650"
     CDS             558..764
                     /locus_tag="VFQ79_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139964.1"
                     /translation="MKKLLVIAAGVLAVAGSSTAGLLNWNPVSQLTDLRLAAAFGLLP
                     ESSVYLAIGMALVSVPLLTRRSRG"
     gene            complement(776..1606)
                     /gene="prmC"
                     /locus_tag="VFQ79_09655"
     CDS             complement(776..1606)
                     /gene="prmC"
                     /locus_tag="VFQ79_09655"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU0139965.1"
                     /translation="MTVQTALLQGAKILDESGIPAARLTAEVLLSHALGRERSYLYSY
                     PEADIGDLAWLHYGRYLHERLQGKPTQYITRRQEFYGREFRVSPDVLIPRPETEHVIE
                     QVLALDPRPSRIVDVGCGSGAIAVTLSLELGCAVFATDISPAAAAVARANATALGATV
                     HIVVCDLLAPVGTHECDLVVSNPPYVPKSQMESLQREVRDYEPVTALLAGEAGTEFYE
                     RLADEGKRVLRPGGYIVLELGFSFSEPVQSIFGQGWEHISLLPDLAGIPRVFTARNAG
                     "
     gene            complement(1572..2681)
                     /gene="prfA"
                     /locus_tag="VFQ79_09660"
     CDS             complement(1572..2681)
                     /gene="prfA"
                     /locus_tag="VFQ79_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU0139966.1"
                     /translation="MQYVQKLDELETRFEDLTRQMADPSVINDPENYRKIAKTHSELT
                     EVVTRYRDYKRIRHNLDDARLMLAENDPDLRHMAEEEIEALEPEMSGLEEQLKLLLLP
                     KDPNDDRDVVLEIRAGAGGDEASLFAAEMFRMYSRYAEALNWRVELTSSSESGAGGVK
                     EVIALISGDKVYSRLKYESGVHRVQRVPVTEQQGRIHTSTVTVAVLPEADDVEVSIDP
                     GDIRIDTFCSSGPGGQSVNTTYSAVRITHLPTGLVVSCQDEKSQIKNRAKAMRVLRSR
                     LYEIELQKQLAQIGADRRNMVGTGDRSEKIRTYNFKENRVTDHRIGLTLYQLDQVIEG
                     RLDPIVDALTTYYQTEKLKEQAATDDRSNGAAPRR"
     gene            complement(2718..3695)
                     /locus_tag="VFQ79_09665"
     CDS             complement(2718..3695)
                     /locus_tag="VFQ79_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEU0139967.1"
                     /translation="MSFHKFVRLAAHLQMLPVLESGEETLVGGQAVMEGVMMRAPHSY
                     CVAVRKPSGEIVTQEHALPRLSEQFPVFRWPLLRGIGTLGQAMKLGVRALRFSANAAM
                     DTGKEGAGKDEVPGWMMALNIAFSLGFFIFLYKFVPLWITTEIGGWFPAFNAPVLFNL
                     TDGIIRVAIFLAFLLLISRWQDIRRVFEYHGAEHRVVFNFESGRPVNIENARSFRTFH
                     PRCGTSFLLVVMVISIAVYALVPVEGFLAKFVARILLLPVIAGLSYEMIRFAAKRQGG
                     LLSALTAPGLWLQRVTTKVPDDRQTEVAIHALDRAMEIEKAHGGELVVA"
     gene            complement(3731..5269)
                     /locus_tag="VFQ79_09670"
     CDS             complement(3731..5269)
                     /locus_tag="VFQ79_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0139968.1"
                     /translation="MRVHLINPSDVSFGTGVIMPRWLYVLAGATPPQYGDPLICDETL
                     QQFDLASIEPGDVAGIGIHTANARRGFELGRLLRERGAWVVYGGIHATLYPEEASEMG
                     SAHAVVKGDGDGVWRTVLADCEKGRPEPLYDGGRVPATDFVAARWDLLPKGRYMWGSV
                     QTVRGCPKHCSFCSVWRTDGQAPRQRRADAVVREVVALRRLGYRFIALADDNFYPVTL
                     GDLELARKQNNHERLAMLTAIRNERFELMEMLSEVSGDLILFTQITMEAAEDPAFLKA
                     MAKAGIKGGLVGVESVTPEGLRAVYKQFNASGDELAARLRVFREHGVHVLGSFIFGLA
                     TDRAETFAATADLAKKADLTFAQFVTMTPFPGTVDFQRWEESLGDNAERIDGIPITRY
                     WLLPGHLRPKLYMPHPSMTPEEIRRGTQGVWDDFYSIKEIWRRSRCVKSMKARLAFVF
                     ISKLYRQMYAKTGIATDSARRQSSTRWARWLARPCQRLFAGKPMPELRVPASRASAFD
                     IVGQ"
     gene            complement(5405..6169)
                     /locus_tag="VFQ79_09675"
     CDS             complement(5405..6169)
                     /locus_tag="VFQ79_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0139969.1"
                     /translation="MNSEFKGKTALITGASRGIGTASAIALARNGASRVVLHYNSYEE
                     GAKQAAEAVRTLGAEVRLFQADLASDDGIRKLAETIRAEQPGIDILINNAGSLVKRAA
                     LAEFTPELFDAVMNLNVKSAYFAAQAAAPYMISKGAGVIVNLSSIAARHGGGPGATIY
                     SAAKAAVSTITKGMAKELAPSGIRVNAVSPGTVDNYFHEQFSTKAMLESMAAATPAAR
                     LGTNEEMADVIVFLCSHASRYMYGQTIEVNGGMYMV"
     gene            complement(6254..7030)
                     /locus_tag="VFQ79_09680"
     CDS             complement(6254..7030)
                     /locus_tag="VFQ79_09680"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0139970.1"
                     /translation="MLAVDQNLRHAMTSFSLVSSSGEVRGLAGVQLVSSGIECSVFNT
                     AFLSGPVHNTGELERRLAIAQVHFAARRLPWSFWICEDLLENGVRGVYRDILPRRGLR
                     LLSDAPGMIADELRPPVRELQPVQCLPVSDERTRGAFARITSFCFDLPMELACRIYEC
                     SRFWMGDMSGYVAYVDGEPVSTMAVVVAAGVVGVYSVATLPMWQGKGCAEALMRQVLS
                     LKQQETGITRSILQSTSAGVSLYTRLGYRNVTRFAVFIST"
     gene            complement(7038..9446)
                     /gene="priA"
                     /locus_tag="VFQ79_09685"
     CDS             complement(7038..9446)
                     /gene="priA"
                     /locus_tag="VFQ79_09685"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU0139971.1"
                     /translation="MARYCDVSLPVPLDRPFTYAVPDTMSHRIQAGCRVLVPFGTRRL
                     IGMVIGVHDGDPETASREALRLVDEEPVLDSSLLGLGAWISSYYSAPLGEVLRTMVPL
                     TGELRQGKIYSLSDSGRDAARQLLFDDGGTEAAAQVLRALEKRPLTAAYLASKIPGAQ
                     RALRSLEKRGFVEVEESHDTRDPRRASSSRLVVEFAERPGSVKLGKPERELSAYLELH
                     PGAHSLKELERVVPRASTAARLLARKGFVRLVLREPVLMAPERGPHKLNPAQQEAFRA
                     VHAAIESSEFRTILLHGVTGSGKTEVYLNAIDSALALGRGALLLVPEIALTPAVAGQF
                     FGRFGNRVAILHSAFSGIERADQWRRIQRGDAPVVVGTRSGVFAPVPNLGLIVVDEEH
                     DQSYKQEETPRYNGRDVAIVRAQQTGACVVLGSATPSLESRYNVERGKYTLITLPERI
                     ENRPMPEVEIVDMRLEFLETRKQAVFSRKLIEQIGRRLETGEQTMLLLNRRGFSAFVA
                     CRACGERIQCVNCAVTLTFHRRDRRLLCHYCNYAERVPSVCPKCNSEYLQFLGTGSEK
                     VEEELHREFPAARIARMDRDTVTGKRQYETILNGFRERRYDILVGTQMIAKGHDIPNV
                     TLVGVVSADVGLAMPDFRAAERTFQLLTQVAGRAGRGSVAGRVLVQTINPDHYAIRFA
                     AAQDYASFYEKEIEFRRLMRYPPFSALANVVVRGRKQEDALRMSAELERILNPAPERT
                     RILGPAEAPVPRLRDEFRYQLLIKTGSRKILRELLDRLRSHALDNKWPATALVIDVDP
                     LSLL"
     gene            complement(9482..10978)
                     /gene="xylB"
                     /locus_tag="VFQ79_09690"
     CDS             complement(9482..10978)
                     /gene="xylB"
                     /locus_tag="VFQ79_09690"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEU0139972.1"
                     /translation="MYWLGIDIGTGGSRAILVDDKGRVKAGFTAPHEDMRMERPLWAE
                     QRPENWWDAAQNAVRAVIKEAKVKPGDIGAVGLSGQMHGLVILDEKDAVIRPAIIWCD
                     QRSQSQVDAINQKVGRDNVLAFTANPVLTGFTLPKLLWVRDCEPKNFARVRKLLLPKD
                     YIRFKLTDEYATEVSDASGTALFDVVTRRWSEDMADRLNLDRGILPQVFESSEVTGQI
                     TAEAAQATGLKAGTPVVGGGGDQAASAVGNGIVAPGIASCTLGTSGVVFAHMEKPNYD
                     PQGRVHTFCHAVKGKWHVMGVTQGAGLSLQWFRNQLAPGTEYDALMAEAASSPASAHG
                     LFWLPYLMGERTPHLDATARGGWIGLTARHTRADLIRAVIEGVSFSLKDCLDTIEGMG
                     LQVDSIRSSGGGARSPIWRQILADTFGKKVTTLETQEGSAYGAAILAAVGAGAYASVP
                     EACGAVIREVDSIAPRPHESQLYRRSHEVYRSLYPTLRPIYSLIRNLG"
     gene            complement(10992..11918)
                     /locus_tag="VFQ79_09695"
     CDS             complement(10992..11918)
                     /locus_tag="VFQ79_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0139973.1"
                     /translation="MAEYSIGVDLGGTNLRAAAVTKDGTIVEKISGSTNLSEGRDAVI
                     SDIVDAITTIKRKVGASRLAGVGIGVPGFILIEKGIIVGSNNLPEFEGFPVRDEIERL
                     LGTQVILENDANAAAMGEKWMGAGKEVDDLILLTLGTGIGGGIISHGRILHGFVGMGG
                     EIGHMTVYPNGNPCGCGNYGCLEKHASATAVSSMAALLHLGDDVTSEDVYKMAAAGDE
                     KAQMIFVSMGMALGLAIGTLVNIFNFPLVLLSGGMLPAWDLFAPPMMKEVERSFTFRN
                     TKTTVGKAILGNEAGLYGAAYLPFQALTATIS"
     gene            11976..13397
                     /locus_tag="VFQ79_09700"
     CDS             11976..13397
                     /locus_tag="VFQ79_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0139974.1"
                     /translation="MALSRRELLTGAAAAEHLRASAAEPERPNVVMFMTDDHGAWATG
                     AYGCGSIHTPHIDRLAATGARFTRAFACTPVCSPSRMTYMTGALPSRHHVQDWLIPAD
                     SFGPASRRWLEGFRTFPEVLAANGYSLGMCGKWHMGDDDKAQAGFSYWATVPGGGGPY
                     TDAEFVYNGVRRKIAGFKTDIQTDFALDFLDRSHKQPFFLFVPFYAPHTPYNFQPEVD
                     RRHYAESRFSCFPDPPPHPWQNKGLARMHGTREAKLAYSALISGVDRNVGRVLEKLEK
                     LGVRENTLVIFTADQGWNAGHHGVWGKGNGTVPFNMYEQSIQVPLIWNHSGRIRGGET
                     PDPMVSSYDFFPSLLDYLGMKASPDARRVGRSYAPFLRGAKPSWRNRLFFEYAYVRAL
                     RTETLKYIERTEGYPSELYDLESDPEETRNVLNDAAYARPLADLRSELKGFFQKAGAP
                     PLDDWRTTTTQILPAESRRPASP"
     gene            complement(13437..15377)
                     /locus_tag="VFQ79_09705"
     CDS             complement(13437..15377)
                     /locus_tag="VFQ79_09705"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0139975.1"
                     /translation="MSARRTALLRQAAADQPHDYFLLRRLLQAEEKQDEQLRIAKEWR
                     AKYPGRPVYVVLHARALMGRDTREALRMLEEMKAAHPEIPFVHTEIVRATGWGKFKDA
                     ARAKQELEAYLKQCLAPLDSESLSSVMQAGSQAQTASVAGAVRARLEKESDPLLSGVW
                     EMLWRLEFKATPPNEHDALRRRIAAELAHFEKAPQRGELRWRSFLRGGYERAGEPASV
                     KRLEDEILKENPTSRAAKGILQDRWRKEHPYPPNPNKATLEAWNRQRLAINREWHQRW
                     PKDSLILDGILGALNQLPDTQAEQVAATGDKLMEEYRRNPNWWGSPPTEFRVANAFLK
                     HKVNLAKVPGLVEEGIRSSTQRRDEQLADDRLPDEMRIMMRESAETLRIDRARILLDY
                     YAAVQKPEGAREIETELASMQPVKPQAKSNLLARRAQAAELSGRKLDALLLYRAALDS
                     RPVDAPKPVDGKDLVADNFERLWKELGGTDVSRALLMGHGKKTETATQSRWERPKNPI
                     PSFSVVDLEGKTWKLAALEGKVVLINLWATWCGPCRAEHPEFQKLYDKLKGRADVSVV
                     TFNIDDDLGKVAPYMKENKYTFPVLLAREVVDAVLPVIAIPQNWFVNAKGKLEWLQVG
                     YGSEPTWQESMLAKLEEVRKGG"
     gene            complement(15639..16121)
                     /locus_tag="VFQ79_09710"
     CDS             complement(15639..16121)
                     /locus_tag="VFQ79_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139976.1"
                     /translation="MFLRSAIMFGTLLLSSGVFGQSKYSGPRPPKPDVPYLLHAENLV
                     PTETAEAKEESRKNNTTYTVPGAASSARTPLAEPIFLFQSQSISPEKLQLYQLEVKGG
                     NREIEFPENRNRAPRPYPLSITRLAEGLYRVEASATLENGQYVLTPTGSNTVFLFEVY
                     "
     gene            complement(16143..18530)
                     /locus_tag="VFQ79_09715"
     CDS             complement(16143..18530)
                     /locus_tag="VFQ79_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0139977.1"
                     /translation="MASRTRVHLIQSAAAAVCLLLSCAPLVAQQQISIREAASRRPSD
                     FAPVHQGKDVLVTGTVTGKAIALLDYAHLGITDGEGHGLTVEDYYDQLVKFEPGDRLQ
                     VQGRIGKRGGVPVVRPTEIRLISHTEPPAAYDVTAEELNSPRYLGLWVTTQADVIEIG
                     EDAGGDYLVVGDPGSMVKVFLPKRARDDYNSLAQFAPRDRVRITGLASQYCPIPPYDR
                     MFQVLVAGADSVVLVNRRWLIPPGILLLSVVLLVAALGVWLYRERHVREHRESIRALH
                     GLAENVIAAGSPSEILDRLCAALPRIFGMTGVTFYIYREAERTMERVASPETTIPPFF
                     LDSPPDPITGGAAICIRNRTLLVVPETHRSPFFSGSGQDLLPRSVIFVPMFWQAELMG
                     VLQLQHDTEARNFNIDQQTAVQHLANQIATSLKLLEQRSIREQLFRSEKLAASGQLIS
                     GVAAELGAPIDGIVTAAGQLLSRSADKRLRRDLSIIAEQARKASEIVTRLVSFANADK
                     TQPKPIDVNSILAGLIDFREREWKLKEIELVNRVSTDRMYVTAAQGQLEQVFLSLILR
                     AEQALADAAEKRLSLASSELAGRVIVEIAFTAPKPTEAIDLLNPDPDGAAGVDVSRAI
                     IRAYGGELRSGGSRKGQKFEVELPAARQREVVLDPGEETLPGSERSLTALLVEPDPIV
                     RRQLLLMVTARGHRVVPVAQAEQAVEMVPRLRFDAVFCSVRLPGLNWVEFFERVRHLV
                     GCFVLVTEGSDPELSRTFESGDGYVLTKPINDGEVKTLLSTIARRDLKREAKPVG"
     gene            complement(18503..19144)
                     /locus_tag="VFQ79_09720"
     CDS             complement(18503..19144)
                     /locus_tag="VFQ79_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0139978.1"
                     /translation="MKFIFDLDGVIVHSTPLHVKAWEAYLEPLGIDSTSIGRIMLGRH
                     NDDIVRELFGSALDPTEICRHGAAKEAIYREMIRPHVNEYMVPGVRPFLERHAGVDKA
                     VASNAERANIDCILRESGLDRFFRVVLDGNQVSRPKPDPEIYVCAAAMLDISPEECVI
                     FEDSKAGIEAARSAGASVVGLRTTCADLTGVDLAIDDFFAPELEEWLREQEST"
     gene            complement(19194..20855)
                     /locus_tag="VFQ79_09725"
     CDS             complement(19194..20855)
                     /locus_tag="VFQ79_09725"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0139979.1"
                     /translation="MKELRGTAVIIGAGPAGLTAALELLRRAKVKPIVLEKSGYMGGI
                     SRTVNYKGNRIDIGGHRFFSKSDRVMNWWTEVMPVEDTTDENHRLVYQNRERTLAATG
                     KKGPDPKSEDLVLLVRPRQSRILFLRKLFEYPLKLTPVTLRQLGLLRTFRAGISYLRS
                     SIRPIPNEKTLEDFLINRFGRQLYRTFFKSYTEKVWGVPCDQISAEWGAQRIKGLSLR
                     TAILHHLRKLFGRGSKDIAQKDTETSLIEKFLYPKFGPGQLWEEVSRRVRAEGGEILT
                     GWNVARIHASGDRVTGVTAVNEQGEERHFPADHVFSTMPVKELIHALDVEIPREVREV
                     GDGLIYRDFITVGLLLDRLRLGEKGRKNSEPLKDNWIYIQEHDVLVGRMQIFNNWSPF
                     MVQDPAKVWVGFEYFCYDTDDLWKKPDKEMIELAIEEAERTDIFSRGDVVDATVLRME
                     KTYPAYFGTYDRFDVIRSFVDGFHNLFLVGRNGMHKYNNQDHSMLTAMTAVDNIIAGR
                     HDKSNIWAVNTEMEYHESKDAAAEDSSRSGAVRAPAVRTVRESPQ"
     gene            21155..21637
                     /locus_tag="VFQ79_09730"
     CDS             21155..21637
                     /locus_tag="VFQ79_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139980.1"
                     /translation="MAIYVALFPIFPAGCRRALKDSASLVEMGNLSVSNQLLSGFHDI
                     EMERWRWTAQSFSVTLKPPSASEQNGATLLLRLFIPAVQIDKLGPITLSSEVDDQVLD
                     PQTFSKPGEYTYARDLPPALLATNVLPIRFCLDRATPGTETDGRELGIVVTSVGLISK
                     "
     gene            21735..22769
                     /locus_tag="VFQ79_09735"
     CDS             21735..22769
                     /locus_tag="VFQ79_09735"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0139981.1"
                     /translation="MKTILVTGGAGFFGGVLKREVLAAGHRCVSVDIYPDEDSHRNLI
                     THQLDIRDGEALDRLFASEKIDGVVHCAAILAHGSEDHGFLWSSNVEGTRSVALAMRK
                     HGVRQLVFTSTNCLWGEALKRPVREDDTPNPIEIYGRSKLEAEKVLGEFTDLNAVIIR
                     CPTIIDFGRLGLLSILFEFIDEGRRVWTVGGGRNRYQFIYAKDLAQACLLALDYPESE
                     TFNIGSDDVKSLAEVYEYVIRRAGSKSSVRSLPRFPAILAMKLAYLLKISPLGPYHYK
                     MIAEDFLFDTSKIQRLLHWRPTMTNEEMLWRAYEYYSRNRREIENRRNVSAHRKGAQM
                     GVIRLLKWVS"
     gene            22757..>23165
                     /locus_tag="VFQ79_09740"
     CDS             22757..>23165
                     /locus_tag="VFQ79_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139982.1"
                     /translation="MGVIAAWPRRAIRTDTLPQLAVYCAGALYILVLFLTVSHHEPWA
                     DEAQSWLLARDLSLAQLLAVAPHYEGTPPLWHLLLHLLIRLGLPYSALNIVSGALGVA
                     AAWLIVRYAPLPLWIRLVLPCTYFLGYQYPVIAR"
BASE COUNT         4611 a         7127 c         6726 g         4701 t
ORIGIN      
        1 ccgccctttt gacggatttc gagttcagcg cttgcctgcc gcgcgcgagc ctcagccgca
       61 gcgagatcgg tcacggcatc gatggattcc aattccacca gcgcggcccc ttttaccacc
      121 acctgtccct gttgcaccag gacgcgctgc accgggccgg ccttctgcgc atgcactgcc
      181 gtccactccg ccggatcggc ctttccgttg gtggccagca tgtccaccag tgtgctgcgt
      241 gtcaccgggg cgaatggcac ctcaggcggc gcggaccggc ggctccatat ccatacaatg
      301 gctccggaaa caacgagcaa cgtcagcaga agtaggattt ttcgcaagct tgccgtgttc
      361 ctccgggctc tctttcataa ttgcatatgc gccggcgagc cggtctcaat ggcgcagcta
      421 agttcatagt tacaaacaga ataactttga ctgcccggtc agattgtctt atactggatt
      481 cagtatcggg gctgaaggct aacaaaaaat aggacccgaa catattggtg tagtacctgg
      541 tcaactcgga ggaagtagtg aaaaagctgt tagtaatcgc tgctggtgtg ctcgcggtgg
      601 ctggaagcag caccgccggc cttctgaatt ggaatccggt ctctcaactt actgatttgc
      661 gtttggcggc cgcgttcgga ttattgccgg aatccagcgt ttatctggcg attggtatgg
      721 cgctggtttc cgtgccgctt ctgacccgcc gctccagggg ctgaggcgtc ccggattatc
      781 ctgcattccg cgcggtgaag acgcgcggaa tgcccgcaag gtcgggcaga aggctgatgt
      841 gctcccatcc ctgcccgaaa atggactgga ccggctcgga gaagctgaag ccgagctcaa
      901 gtacgatata gccgccgggg cgcaacacgc gtttgccctc atccgctagc cgttcataga
      961 attccgtgcc agcctccccc gccaatagag cggtaacggg ttcatagtcg cgaacttccc
     1021 gctgcaagct ctccatttgc gatttgggaa cgtagggcgg attggaaaca accaggtcgc
     1081 attcgtgcgt cccgaccggc gccagcagat cgcacaccac aatgtgcact gtagcgccga
     1141 gcgccgtcgc gttggcacgg gcgacggccg ccgccgccgg cgagatatcc gtcgcaaaca
     1201 ccgcacagcc cagttcaaga ctcaatgtga cagcgatggc gccggaaccg caccctacgt
     1261 ccactattcg cgaggggcgg gggtccagcg ccagtacctg ctcgatgacg tgctcggttt
     1321 ccggccgcgg gatcaataca tcgggagaaa cacggaattc acgtccataa aactcctgcc
     1381 tgcgagtgat gtactgcgtg ggtttgccct ggagccgctc atgcagatag cggccgtagt
     1441 ggagccaggc gagatcgccg atatcggctt cggggtaact gtagaggtat gagcgttcac
     1501 ggccgagagc atgagacagc agaacctcgg cggtgagccg cgcggcgggt atgcccgatt
     1561 cgtccagaat cttagcgcct tggagcagcg ccgtttgaac ggtcatccgt tgcagcttgc
     1621 tctttcaact tttccgtctg gtaatacgta gtcaaagcgt cgacgatggg gtccaacctg
     1681 ccctcgatca cctggtcaag ctggtagagc gtcaacccga tccgatgatc cgtcacacga
     1741 ttctctttga agttgtaggt gcgaatcttc tcgctcctgt cgccggtgcc caccatgttc
     1801 ctgcggtcgg cgccgatttg tgcgagttgc ttctgtagct cgatttcgta caatcggctg
     1861 cgcagcacgc gcatcgcctt cgcgcggttt ttgatctgcg acttttcatc ctggcacgag
     1921 acgacaagtc cggtgggcag gtgggtaatg cgcacagcgg aatacgtggt gtttaccgac
     1981 tgtccgcccg gacccgagga acagaacgta tcgatgcgga tgtcgccggg gtcaatggaa
     2041 acttcgacgt cgtccgcttc tggaagtacg gccacggtca cggtggaagt atgaatacgg
     2101 ccctgctgct ccgtaaccgg cacccgctgt acgcggtgca caccgctttc gtatttgagc
     2161 cggctgtaga ccttgtcgcc gctgatcagg gcgatgactt cctttacgcc cccggcgccg
     2221 gattcgctgg aagaggtgag ttccacgcgc cagttcagcg cttcagcata tctcgagtac
     2281 atgcggaaca tctccgccgc aaacaggctg gcctcatctc cccccgcgcc ggcgcggatt
     2341 tcgaggacaa cgtcccggtc gtcattgggg tctttcggca gcagcaggag tttgagctgc
     2401 tcctccagcc cactcatttc gggctccagc gcctcgatct cctcctccgc catatggcgc
     2461 aggtcggggt cgttctcagc cagcatcagc cgggcgtcgt cgaggttatg ccggatgcgc
     2521 ttgtagtcgc ggtatcgtgt caccacctcg gtgagttcac tatgcgtctt agcgatcttg
     2581 cggtagttct ccggatcgtt gatgacggac ggatcggcca tctgccgggt gagatcctca
     2641 aagcgggttt ccaactcgtc gagcttctga acgtattgca ttgccaggta agggccggcg
     2701 cggctaccgg ctgcgtttta agctaccacc agttctccac cgtgagcttt ctcaatctcc
     2761 atcgcccggt ccagagcgtg gatggccact tccgtctgcc ggtcgtcggg aaccttcgtc
     2821 gtcacgcgct ggagccaaag gccaggtgcg gttaacgccg acaacaaccc gccttgacgc
     2881 ttcgccgcaa aacgaatcat ttcgtagctg agccccgcga taacgggtag cagaagaatg
     2941 cgcgccacga acttggccag gaacccttcc actggaacca gggcatacac ggcgatggag
     3001 atcaccatca ccaccaggag aaaactcgtg ccgcaacgcg ggtggaacgt gcgaaacgaa
     3061 cgcgcgttct cgatattcac cggccgcccc gattcgaaat tgaacaccac tctatgctcg
     3121 gccccgtgat attcaaatac gcgccggata tcctgccatc gcgaaatgag cagcaggaaa
     3181 gccagaaaaa tcgccacccg gatgattcca tccgtcaggt tgaacagtac cggtgcgttg
     3241 aaagctggaa accagccccc gatctccgtg gtgatccaga gcggaacgaa cttgtacagg
     3301 aagatgaaaa atccaagcga aaacgctata ttcagcgcca tcatccagcc cggaacttca
     3361 tctttcccgg cgccttcttt gccggtgtcc atcgcggcgt tggccgagaa ccgcagcgcc
     3421 cggacgccta atttcatagc ctgtccaaga gttccaatac cgcgcagaag gggccagcgg
     3481 aagacaggaa actgttcgga gagccgcggc agagcgtgct cctgcgtcac gatttcgccg
     3541 cttggttttc tcaccgctac acaataactg tggggggccc gcatcatgac gccctccatt
     3601 accgcctgtc caccaacaag agtctcctct ccgctctcga ggacaggaag catctggagg
     3661 tgcgcagcca gacgcacgaa cttatggaat gacaaggtgc aggaactcct acgctccagt
     3721 atatcggaaa ctattggccc acgatatcga aagcggaggc gcggcttgca ggcacccgca
     3781 gctccggcat cggttttcct gcaaacaggc gctggcatgg tctcgcgagc catcgcgccc
     3841 agcgcgtcga cgattgcctc cgggcgctgt cggtggcaat tcctgtcttg gcgtacatct
     3901 gccggtatag cttcgaaatg aaaacgaacg caaggcgcgc cttcatggat tttacacagc
     3961 gcgagcgccg ccagatctcc ttgatactgt agaagtcgtc ccagacgccc tgtgtccccc
     4021 gccgaatctc ttccggggtc attgacggat gcggcatata cagttttggg cgtaaatgtc
     4081 cgggcagcag ccagtagcgc gtaatgggaa taccatcgat gcgctcagcg ttatcgccga
     4141 ggctttcctc ccagcgctga aaatccaccg ttcccggaaa tggcgtcatg gtcacgaatt
     4201 gcgcgaatgt cagatcggcc tttttcgcga ggtcggctgt cgcggcgaag gtctccgcac
     4261 gatccgtcgc cagcccgaag atgaaggacc cgagaacgtg cacgccgtgt tcccggaata
     4321 cccgaagccg cgccgccagt tcatcgccgg aagcgttgaa ctgcttatag acggcgcgca
     4381 atccttccgg cgtcaccgat tcaacaccga ccagcccgcc tttgatcccc gccttggcca
     4441 ttgctttcag gaacgccgga tcttcggccg cttccatcgt gatctgcgta aacaggatca
     4501 gatccccgga gacctcggac agcatctcca tcagctcaaa acgttcatta cggatggccg
     4561 tcaacatcgc cagccgttcg tgattgttct gcttgcgggc gagttccagg tcgcccagcg
     4621 ttaccgggta aaaattgtca tcggcgagcg cgatgaaacg gtaacccaac cggcgcagcg
     4681 ccacaacctc acgaaccacc gcgtccgccc ggcgttgccg gggggcctgt ccgtcggtgc
     4741 gccacacgga gcagaaggag cagtgcttcg ggcaccctcg cactgtctgc acagagcccc
     4801 acatgtagcg gcccttcggc agcagatccc accgtgccgc gacgaaatcc gtggccggca
     4861 ccctgcctcc gtcgtacagc ggttccggcc ggcccttctc gcaatccgcc agcactgttc
     4921 tccacacgcc gtcgccatcg cctttcacaa ccgcgtgcgc cgaacccatc tcgcttgcct
     4981 cttccggata gagcgtggcg tgaataccgc catataccac ccacgcaccc cgttcgcgca
     5041 gcaggcggcc caattcgaag cctcgccgcg cattcgccgt gtggatacca ataccggcta
     5101 catcacccgg ttcgatggaa gctaaatcga actgctggag cgtttcgtcg caaatcagcg
     5161 ggtcgccgta ctgcggcggc gtggcaccgg ccagaacata cagccagcgc ggcatgatga
     5221 cgccggtccc aaaggagacg tcacttggat tgatgagatg aacacgcaca atctgctcct
     5281 ttcaagttcg cgtcagagaa catctggagg cagaatacac gtgtccagca tcgcgaacgc
     5341 tacacgatag tatcaggtaa cgccagggat gtcccggacg tggctcgccg cccatcgaga
     5401 gggatcatac catgtacatg ccgccgttga cctcgatcgt ttggccgtac atgtagcgcg
     5461 atgcgtgtga acacaggaat acaataacgt cggccatttc ctcattcgtc ccgagacgcg
     5521 ccgccggcgt agccgcggcc atggattcga gcatggcttt ggtggaaaac tgctcatgaa
     5581 agtagttgtc cacggtgccg gggctcacgg cgttcacacg aatcccgctc ggtgcgagtt
     5641 ccttcgccat ccctttcgta atggtggaga cggccgcttt tgccgccgaa tagatggtgg
     5701 cgcccggacc tccgccatgc cgggccgcaa tcgagctgag atttacgatg acgccagcgc
     5761 ctttggagat catatacggc gctgccgcct gagcggcaaa ataggcactc ttgacgttga
     5821 ggttcatcac cgcgtcgaat agctccggtg tgaattcggc gagcgcggcc cgcttcacca
     5881 gggaaccggc attgttgatg aggatgtcga tgcccggctg ctcggcgcgt atcgtctcag
     5941 ccagtttccg gatgccgtcg tccgaggcga ggtcggcctg aaacaatcga acctccgcac
     6001 ccaatgtgcg caccgcctcg gcagcctgct tcgcgccttc ttcataagag ttgtaatgaa
     6061 gcacgactcg cgaggctccg ttgcgcgcga gcgcaatagc cgaggcagtg ccgattccgc
     6121 gtgacgcgcc cgtgataagg gcagtcttgc ccttgaactc ggaattcaaa gtttcctcca
     6181 tagtcaatcg gtgaaccaac atactacgat actttgccgg gaccgtccgg gaccaaggtt
     6241 accaggaagg ggatcatgta gagatgaaga cagcgaaacg ggtgacattc cggtatccga
     6301 ggcgcgtgta cagagagact cccgcggagg tcgattgcag gatactgcgg gtgattccgg
     6361 tctcctgttg tttcagggaa aggacctggc gcatcaacgc ctccgcacag cctttgccct
     6421 gccacatggg cagtgtcgcc acggaataaa cgcccactac tccggcggcg acaacaacgg
     6481 ccatcgtgct gacaggctcg ccatccacgt aagccacata tccggacata tcgcccatcc
     6541 agaaccggct gcattcgtag atccggcacg cgagttccat cggaagatcg aaacagaagg
     6601 acgtgatgcg agcgaaagca ccgcgggtgc gttcatcgga caccggcaga cactgaaccg
     6661 gctgcaactc acgaacggga ggccgcagtt catcggcaat catccccggc gcgtcgctga
     6721 gcaaccggag tccgcgccgg ggaaggatat cgcggtacac gccccgcacg ccgttctcga
     6781 gcaaatcctc gcagatccag aacgaccacg gaaggcgccg cgcggcaaaa tgaacctggg
     6841 ctatcgccag acgccgctcc aattcgcccg tgttgtgcac aggtccggaa agaaaggcgg
     6901 tgttgaaaac actgcattcg atgcccgaag agacgagctg aaccccggcc aggccgcgga
     6961 cctcgccgct ggaactcacg agggaaaacg atgtcatcgc gtggcgcaga ttttggtcga
     7021 cggccagcac tgtccgatca gagcagactc aacgggtcaa cgtcaatgac gagggccgtg
     7081 gccggccact tgttatcgag cgcatgggag cgtaaacggt cgagcagctc tcgcagaatc
     7141 ttccggctgc cggtcttgat aagcaactgg tagcgaaact cgtcgcgcaa gcgtggaacg
     7201 ggggcttcgg cgggccccag aatcctggtg cgctccggcg cgggattgag gatgcgctcc
     7261 agttcggcgc tcatacgcag ggcgtcttcc tgtttccggc cacgtacgac cacgttcgca
     7321 agcgcgctga acggaggata ccgcatcagg cgccggaact cgatctcctt ttcgtaaaac
     7381 gatgcatagt cctgcgcggc tgcgaaacgg atggcatagt gatcgggatt gatggtctgt
     7441 accagcacac ggcctgcgac gctgcccctg ccggcgcggc cggccacctg tgtgagcaac
     7501 tggaacgtac gctcagccgc acggaagtcc ggcatggcga gcccgacatc ggccgacacg
     7561 acgccaacca gcgtcacgtt cggaatgtca tgtcccttgg cgatcatctg ggtcccaacc
     7621 agaatgtcgt agcggcgttc acggaatccg ttcaggatgg tttcgtactg gcgttttccg
     7681 gtgacggtgt cgcggtccat gcgcgcgatt cgagctgcgg gaaactcgcg atgcagctct
     7741 tcctccacct tctcggaacc ggtgcccaga aattgcagat actcgctgtt gcacttgggg
     7801 cagaccgagg ggacgcgttc ggcgtaattg caatagtggc acagcaggcg gcggtcacgc
     7861 cggtgaaacg tcaacgtgac cgcacaattc acacactgga tccgctcgcc gcacgcacgg
     7921 caggcgacga aggccgagaa gccgcgccgg ttcagcagca gcatggtttg ctctccggtt
     7981 tccaggcgcc ggccgatctg ctcgatcagt ttgcgcgaga aaaccgcttg cttgcgcgtc
     8041 tccaggaact cgagacgcat atcgacaatt tccacttccg gcatggggcg gttttcgatc
     8101 cgttccggca gcgtgatgag ggtgtatttg ccgcgctcga cgttgtaccg gctctcaagg
     8161 cttggggtgg cggaccccag cacaacgcac gctccggttt gctgtgcgcg cacgatggcc
     8221 acgtcgcggc cattgtagcg gggggtctct tcctgcttgt aactctggtc atgctcctca
     8281 tccaccacga tgagaccgag gttcggcacg ggagcgaaca cacccgagcg ggtgcctacc
     8341 accacaggcg catcgccacg ttgaatgcgc cgccattgat cggctcgctc gatgccggag
     8401 aaagcagagt gcaggatggc aacgcggttt ccgaaccggc cgaagaactg gcccgcgacc
     8461 gcgggtgtca gcgcgatttc aggcaccagc agcagcgcgc cgcgtcccag tgcgagcgcc
     8521 gaatcgatgg cattcaaata gacttcagtt tttccggaac ccgtcacgcc gtgtagcagt
     8581 atcgtccgga attcgctcga ttcgatggcg gcatggacgg cgcggaatgc ctcctgctgt
     8641 gccgggttca gcttgtgcgg accgcgctcc ggcgccatca ggaccggctc gcgaagcacg
     8701 aggcgaacaa aacccttgcg ggcgagcaag cgcgccgccg tactggcgcg aggcacgacg
     8761 cgctccagtt ccttcaggct atgggccccg ggatgcaatt cgaggtaagc cgagagctca
     8821 cgttcgggtt tgccgagttt cacgctaccc ggtctttccg cgaattcgac aacgagcctg
     8881 gaggaagagg cgcgtcgcgg gtcgcgcgta tcatgcgatt cctccacttc gacgaaccct
     8941 cgcttttcca gggagcgcaa ggcacgctgc gctcccggta tcttcgatgc gaggtaggcg
     9001 gcggtcagcg ggcgcttctc cagagctcga agtacctgcg ccgccgcctc ggtgccgccg
     9061 tcgtcgaata gaagttgccg cgcggcgtcg cgcccggaat cgctaaggga atagattttg
     9121 ccttggcgca actcgccggt gagcggcacc atggttcgca aaacttcgcc tagcggcgca
     9181 gagtaatagg agctgatcca ggcccccaag ccgagtagcg aagaatccaa cacgggttcc
     9241 tcgtccacca gcctgagggc ttcgcggctt gccgtttcag ggtcgccatc atgaacccca
     9301 atcaccatgc cgatcagtct tcgcgtaccg aacggcacca gcacgcggca acccgcctga
     9361 atgcggtggg acatcgtgtc cggcacggca taggtgaagg gccggtccag cgggaccggc
     9421 aggctcacgt cgcaatagcg ggccatggag cggcacttcc ctcaagcgcc aggcatccgc
     9481 ttcatcccag gtttcggatg aggctgtaga tcggccgcaa cgtgggataa agggacctgt
     9541 agacttcgtg cgaccgccgg tacaactgcg attcgtgcgg ccgtggagcg atggaatcca
     9601 cttcccgaat caccgcgccg caggcttccg gaaccgatgc gtacgcacca gcgcccacag
     9661 cggccagaat cgctgcgccg tacgccgagc cttcctgcgt ttcgagcgtg gtcaccttct
     9721 tgccgaacgt gtcggccagt atctggcgcc agatggggct gcgcgcgccg ccgccggatg
     9781 agcgaatgga atccacctgc aggcccatgc cttcgatcgt atcgaggcag tccttcaggc
     9841 tgaaagaaac cccttcgata acggctcgaa tcagatccgc gcgggtgtgc ctggcggtca
     9901 ggccgatcca gcctccgcgc gccgtggcgt cgagatgggg ggtgcgctcg cccatcagat
     9961 acggcagcca gaagagtccg tgtgcgctcg cgggggaaga tgcggcctcc gccatcagcg
    10021 catcgtattc ggttccgggc gcaagctgat tccggaacca ctgcaagctg agtccggctc
    10081 cctgcgtgac ccccatcacg tgccacttgc ctttcactgc gtgacaaaac gtatgcacgc
    10141 ggccctgtgg atcgtagttg ggcttttcca tgtgcgcgaa gaccacaccg gaagtaccta
    10201 gcgtgcagga ggcaatgccc ggtgcgacga tgccgttgcc aaccgcgctg gccgcctggt
    10261 ctccacctcc gcccaccacg ggagtgcccg ccttcaagcc tgttgcctgc gcagcctccg
    10321 cggtgatctg gccggtgact tcgctcgatt caaatacctg ggggagaatg ccgcgatcga
    10381 gattcaaccg gtctgccatg tcttcggacc agcgccgcgt gactacatcg aacagcgccg
    10441 tcccggaggc atccgatacc tccgtggcat actcgtcggt gagtttgaag cggatgtaat
    10501 ccttcggcaa cagaagtttg cgcacgcgcg cgaaattctt cggttcgcaa tcgcgcaccc
    10561 aaagcaactt gggcagcgtg aaaccggtaa gcactggatt ggcggtgaac gccagaacgt
    10621 tatccctacc caccttttga ttgatagcgt ccacctgcga ctggctgcgc tgatcgcacc
    10681 agatgatggc ggggcgaata accgcgtcct tctcatccag gatgaccagt ccgtgcatct
    10741 gtccggagag gcccaccgcg ccaatgtcgc cgggcttcac tttcgcttcc ttaatgaccg
    10801 ctcgaacggc gttctgcgcg gcgtcccacc agttttccgg ccgctgttcg gcccacaagg
    10861 ggcgctccat gcgcatgtct tcatgcggcg cggtgaaccc ggccttcaca cggcccttat
    10921 cgtctacaag gattgcgcgg ctgccgccgg tgccgatatc gatacccaac cagtacatgt
    10981 aaactccagt cctatgaaat tgtagccgtg agcgcctgga acggaagata cgccgctccg
    11041 tacaggcccg cttcgttccc gaggatcgct tttccaaccg tggttttcgt attacggaat
    11101 gtaaaagaac gctccacttc tttcatcatc ggcggagcga aaaggtccca tgccggaagc
    11161 atcccaccac tcagcagcac caaagggaag ttgaagatat tcaccagcgt gccgatggca
    11221 agcccaagcg ccattcccat gctgacgaaa atcatttgcg ccttttcgtc cccggcggcg
    11281 gccattttat agacgtcttc cgaggtgaca tcgtcgccca ggtgcaacag tgccgccatc
    11341 gaggagacgg cggtggccga tgcgtgcttt tcgagacacc cgtagttccc acaaccgcag
    11401 gggtttccgt tcgggtagac ggtcatgtgg ccgatttctc cgcccattcc aacaaacccg
    11461 tgcagaatgc gtccatgcga gatgattccg ccaccgatac cggtgccgag agtgagcaga
    11521 atcagatcgt caacctcttt gccggcgccc atccacttct cacccatcgc ggcggcgttg
    11581 gcgtcgtttt cgagaatgac ttgcgtgccg agcagccgct caatttcgtc gcgaacggga
    11641 aaaccctcga actcgggtag attgttggag ccgacgatga tgcccttctc gataaggata
    11701 aatcccggaa cgccgatgcc tacaccggca aggcggctgg ctccaacctt gcgcttgatc
    11761 gtggtgatcg cgtccacgat gtcggaaatg acggcatccc gcccttccga aaggttggtg
    11821 ctgcctgaga tcttctcgac gatggttccg tcttttgtga ccgcggcggc gcggaggttc
    11881 gtcccgccaa gatccacgcc gattgagtat tctgccatga agccgaaatt ataccgcgcg
    11941 ctatggacag gaagcggatg tatagtacat ggacaatggc gttatccagg cgagaacttc
    12001 tgaccggcgc agcggcggcg gaacacttac gcgcttccgc cgcggaaccg gagcggccga
    12061 acgtcgtgat gttcatgacg gacgatcatg gcgcgtgggc caccggcgcg tatggttgcg
    12121 gcagtattca taccccccat atcgacaggc tcgcggccac cggcgcacga ttcacgcggg
    12181 cgtttgcctg cactccggta tgctctccca gccgtatgac ctatatgacg ggcgcgttgc
    12241 cttcaaggca tcatgtgcag gactggctga ttccggcgga cagtttcggt ccagcgagcc
    12301 gccgctggct ggaaggcttc cgtacgtttc cggaggttct cgcggcgaac ggctactcgc
    12361 tcggcatgtg cggcaagtgg cacatgggag acgacgataa agcgcaggcc gggttttcct
    12421 actgggcgac ggttcccgga ggaggcggcc cctataccga tgcggaattc gtttacaacg
    12481 gcgtccgccg caagatagcc ggctttaaga ccgatataca aacggatttt gcgctcgact
    12541 ttctggaccg gtctcacaag cagccgttct tcctcttcgt cccgttttac gctccgcaca
    12601 ctccgtataa ttttcagccg gaggtggacc ggcggcacta cgcggaatcc cgattttcct
    12661 gctttcccga ccctcctcca cacccctggc aaaacaaggg gctcgcccgg atgcacggaa
    12721 cgcgcgaggc gaaactcgct tattccgccc ttatttccgg tgtagatcga aacgtcgggc
    12781 gcgtgctgga aaagttggag aagctcggcg ttcgtgaaaa cactctcgtc atcttcaccg
    12841 ccgatcaagg ttggaacgcc gggcaccacg gcgtctgggg aaaaggaaac ggcacggtgc
    12901 cgttcaatat gtacgagcaa tccattcagg tgccgcttat ctggaatcat tcgggccgga
    12961 ttcgcggcgg cgagactccc gacccgatgg tatcgagcta cgatttcttt ccgtcccttc
    13021 tcgattacct gggcatgaag gcctcacccg atgcgaggcg cgtcggccgg agctacgctc
    13081 cattcctccg gggcgctaag ccatcctggc gcaaccgcct gttcttcgaa tacgcttatg
    13141 tccgcgcgtt gagaacggaa accctgaaat atatcgagcg cacggagggc tatcccagcg
    13201 agttgtacga cctggaatcc gatcccgaag aaacccgcaa cgtactgaac gatgccgcct
    13261 atgcgcgccc gctggcggat cttcgttcgg agctgaaggg ctttttccag aaagccggtg
    13321 cgccaccgct cgacgattgg cgaacgacca ctacacagat actgcctgcc gaaagcaggc
    13381 gcccggccag tccctgacgg tcatggctcg gaaaccgagc cccgcaacgg ccgatcttac
    13441 ccgcctttcc gcacctcttc cagcttggcc agcatcgact cctgccaagt gggctccgac
    13501 ccatacccca cttgcagcca ttcaagtttg cccttggcat tcacgaacca gttctgcggg
    13561 atcgcgatca ccggcagcac ggcatccacc acctcgcgtg cgagcagcac cgggaacgtg
    13621 tacttgttct ccttcatgta aggagccacc ttccccagat cgtcgtcgat gttgaaggtt
    13681 acaaccgaaa cgtccgcccg ccccttcagc ttgtcgtaga gcttctggaa ctcgggatgt
    13741 tcagcgcggc agggaccgca ccacgtcgcc cataagttga tgagcaccac cttgccctcc
    13801 agggcggcaa gtttccaggt cttgccttcg agatccacca ccgagaagga tggaatggga
    13861 ttcttgggcc gttcccacct gctctgggtc gcggtttcgg tcttcttgcc atgccccatc
    13921 aacagcgcgc gagacacatc tgtgccgcca agctccttcc acaaccgctc gaagttgtcc
    13981 gccaccagat ccttgccatc gaccggcttg ggtgcgtcca ctgggcggga atcgagcgcg
    14041 gcccggtaca gcagcagtgc gtcgagctta cggcctgaca gttcggcggc ctgagcgcgc
    14101 cgcgccagca agttcgattt cgcctgcggc tttacgggct gcatcgatgc gagttcagtc
    14161 tcgatctcgc gtgctccctc gggtttctgt acggccgcgt aatagtcgag gaggatgcgc
    14221 gcccggtcga tcctcagtgt ctcggccgac tctcgcatca tgatccgcat ttcatccggc
    14281 agacggtcgt cggcgagttg ctcatcgcgc cgctgggtcg aagaacgtat tccttcttcg
    14341 accaaccctg gcaccttggc caggttcacc ttatgcttaa gaaacgcgtt cgcaacccgg
    14401 aactcagtcg gcggcgagcc ccaccagttc gggttcctgc ggtactcctc catcagcttg
    14461 tctcccgtcg cggccacctg ttccgcttgg gtgtctggca gttgattcag agctcctaag
    14521 atcccatcga gaatcagcga atccttcggc caacgttgat gccattcccg gttgatcgcc
    14581 agcctctgcc gattccaggc ttccagcgtg gccttgttgg ggttgggtgg atacgggtgc
    14641 tccttgcgcc accggtcctg taggattccc ttcgccgccc gcgacgtcgg attctccttg
    14701 aggatctcgt cctccaagcg cttcaccgat gccggttctc cggccctctc gtagccgccc
    14761 cgaagaaacg atctccaccg cagttcgcct cgctgtggag ccttttcgaa atgcgccaac
    14821 tccgcggcaa tccgccggcg cagtgcgtcg tgttcatttg gaggcgtggc tttgaattcg
    14881 aggcgccaca gcatctccca cacaccgctc agcaggggat cggattcctt ctccagacgt
    14941 gccctcaccg ccccagcaac gctcgccgtc tgagcctgtg agccggcttg catcacgctc
    15001 gacagcgact ccgagtccag cggcgccaga cactgtttca ggtacgcctc cagctcttgc
    15061 ttagcgcgag ccgcatcttt aaatttgccc catccggtcg cgcgaactat ttcggtatga
    15121 acgaagggaa tttcgggatg cgccgctttc atctcttcga gcatacgcaa agcctcgcgt
    15181 gtgtcccggc ccatcagggc acgggcgtgg agaaccacgt agaccggccg gccgggatac
    15241 ttcgcgcgcc attccttggc gattcgaagc tgctcgtcct gcttttcctc ggcctgaaga
    15301 agacgccgca gcaggaaata atcgtgcggc tgatcggccg ccgcctggcg gagcagcgct
    15361 gtccgacgcg cgctcatctc ggacattgtc aaatccaatt cccaaagtcg gccggactgt
    15421 tccagtactt gcctcgtccg ggcggaaggt tcacattgcg gatagagaat gttcgcactg
    15481 caaacgagaa tgagggccag tatgctgatg cggggcatga agctcctcca gccgcaagtt
    15541 taccggattt actcattcat cgcggagtgc ctccatagga tcaatacgcg tggcacgccg
    15601 cgccggaaga gacctttgtc cagtgtgaga atctcgcgct aataaacctc gaacaggaac
    15661 acagtattcg atccggtagg cgtgagaaca tactgcccgt tctcgagggt ggcgctcgct
    15721 tctactctat acagtccctc tgccagcctt gtaatagaga ggggatacgg ccgcggcgct
    15781 ctgttgcggt tttcaggaaa ttcgatttcc cggttgccgc ccttgacctc gagttggtaa
    15841 agctgaagtt tctccggcga aatgctttgg gactggaaca ggaaaatcgg ctcggccagt
    15901 ggcgttctcg ccgaagaagc cgcgccggga accgtgtagg tggtattgtt cttgcgggac
    15961 tcctccttgg cttccgcggt ctccgtcggc acgagattct cggcatgcaa taaatagggg
    16021 acgtcgggct tgggaggcct tgggcctgaa tacttggatt gtccgaaaac gcccgagctt
    16081 agcagcaacg tgccgaacat gatagccgat cgcaaaaaca tactagtcat tgtacggcgc
    16141 gatcagccga cgggtttcgc ttcgcgtttc aaatcccgtc ttgcaatggt ggagagcaaa
    16201 gtcttcacct caccatcgtt aatcggcttg gtgaggacat agccgtcgcc cgattcgaac
    16261 gtgcgggaca gttcggggtc tgagccttcc gtcaccagaa cgaagcagcc gaccagatgc
    16321 cgtacccgct cgaagaactc cacccaattt aatccgggta gccggacgga gcagaaaacg
    16381 gcgtcgaaac gcaggcgcgg caccatctcc acggcctgct cggcctgcgc caccggaact
    16441 acacgatggc cgcgagcggt aaccatcagc agcaattgac gccgtacgat ggggtccggt
    16501 tctaccagca gggcggtcag ggagcgctcc gagcctggaa gcgtctcctc gccggggtcg
    16561 agcaccacct cccgttgacg ggcggccggc aactcgactt cgaacttctg cccttttctc
    16621 gagccgccgg atcgcaactc ccctccatag gcgcggatga tggcacgcga aacatcgacg
    16681 ccggccgcgc catcgggatc ggggttgagc agatcgatgg cttcggtagg cttgggcgcg
    16741 gtgaaggcga tctcgacaat aacccggccc gccagttcgc tggaagccag ggagagccgc
    16801 ttttccgccg catcggccag cgcctgttcg gctcgtaaga tcagacttag aaaaacctgt
    16861 tcgagctggc cctgcgccgc ggtcacgtac atgcgatcgg tactgacacg gttcaccagc
    16921 tcaatttctt tcaatttcca ttcgcgctcg cgaaaatcga tcagtcccgc aaggatggaa
    16981 ttgacatcga tcggtttcgg ctgggtcttg tcggcattcg cgaacgaaac cagccgcgtg
    17041 acaatttcgg aggctttgcg cgcctgttcg gcaatgatgc tcaggtcgcg gcgcaatcgc
    17101 ttatcggcgg agcgcgaaag cagttggccg gccgccgtga caattccatc gatgggggcc
    17161 ccgagttcgg cggccacgcc cgaaatgagc tggcccgagg cggcaagttt ttcgctgcgg
    17221 aacaattgct cgcgaatcga tctctgctcc agcagcttga gcgaggtggc gatctggttc
    17281 gcgaggtgct ggacggcggt ctgctgatcg atattaaagt tgcgcgcctc cgtgtcgtgc
    17341 tgcaactgca gcacgcccat caattcggcc tgccagaaca tggggacgaa gatgacggaa
    17401 cgcggcaaca ggtcctgtcc cgagccgctg aaaaaaggac tcctgtgcgt ttccggcacc
    17461 accagcagag tgcgatttcg aatgcagatg gcggcgccgc cggtgatggg atctggagga
    17521 gaatcgagaa aaaacggcgg tatggtggtt tcgggtgagg cgactctttc catcgtccgt
    17581 tcggcctcac ggtaaatgta gaacgtcaca ccggtcattc cgaaaattct gggcaaggcc
    17641 gcgcacaagc ggtcgagaat ctcggaaggg gagccggcgg cgatcacatt ttcagcgagc
    17701 ccgtgcaagg cacgaatact ctcccggtgt tcgcgtacat ggcgctcccg gtacagccag
    17761 acacccagcg cggcaaccag gaggaccacc gacagcagca ggatgcccgg cggaatcagc
    17821 cagcggcgat tgacgagcac gaccgaatcg gcacccgcca ccagcacttg aaacatacga
    17881 tcgtacggcg gaatcggaca atactgcgag gcaaggcccg tgatgcggac gcggtcccgc
    17941 ggcgcaaact gcgcgagcga gttatagtcg tcgcgggcac gcttgggcag gaacaccttc
    18001 accatggaac ccggatcgcc gacgacgaga taatcgccgc ccgcgtcttc gccgatctcg
    18061 atcacgtcgg cctgtgtcgt gacccacagt cccaggtagc gcggcgagtt cagttcctcg
    18121 gcggtgacat cataagcggc ggggggctcg gtgtgactga tcaggcgaat ctcggttggc
    18181 cgcaccaccg gaacgccacc gcgcttgccg atacggccct gtacctgcaa acggtcaccc
    18241 ggctcgaact tgaccagttg atcgtaatag tcttcgaccg taagcccgtg accttcaccg
    18301 tccgtgatgc ccaggtgcgc gtaatcgagt agcgcgatgg ccttcccggt gaccgtgccg
    18361 gtgacaagca catccttccc ctggtgaacg ggagcgaaat ccgacggacg ccgcgatgcg
    18421 gcttcgcgta tgctgatctg ctgttgcgcc acgagcggcg cgcaactcaa caacagacaa
    18481 accgccgcgg cggcggactg gatcaagtgg actcttgttc tcgaagccat tcttccaact
    18541 ccggcgcgaa gaaatcgtct atagccaggt ctacgcccgt gaggtccgca caagtggtcc
    18601 tcagaccgac taccgaagca cccgcgcttc gcgccgcttc tatgccagcc ttggaatcct
    18661 caaaaataac gcactcctca ggagagatgt caagcatggc ggcggcgcac acgtagatct
    18721 ccggatcggg tttcggcctg ctgacctgat tgccatcgag gaccacacga aaaaaccggt
    18781 caagacccga ctcgcgcagt atgcagtcga tgttcgcccg ttccgcattg gatgccacgg
    18841 ccttatccac gccggcatgc cgctcgagga acggccgcac tccgggcacc atgtattcgt
    18901 tgacgtgagg ccggatcatt tcgcggtaga tcgcctcttt tgcggcgcca tgccggcaga
    18961 tctcggttgg atctagagcg ctgccgaata gctcgcgaac gatatcgtca ttgtggcggc
    19021 ccagcatgat ccgcccgatg gaagtagaat caatgcccaa cggctcgagg taggcctccc
    19081 aggctttgac gtgcagcggt gtcgaatgaa cgatgacccc gtccaggtcg aaaatgaatt
    19141 tcacgcccgt gctccggcac gagaaatact ccacatgccg ggatcataac atttcactgc
    19201 gggctctccc gaacggtacg caccgcgggt gcgcgcacgg cgccgctccg gctggagtcc
    19261 tcggcggcgg cgtccttgga ttcgtggtac tccatctcgg tattgacggc ccagatgttg
    19321 cttttgtcgt gccgtccggc aatgatgttg tcgacggcgg tcatggcggt cagcatcgag
    19381 tgatcctggt tgttgtactt gtgcatgccg ttacgtccca ccaggaacag gttgtggaag
    19441 ccgtctacga agctccggat gacatcgaac cggtcatagg tgccgaagta cgccgggtac
    19501 gtcttctcca tgcggaggac ggtggcatcg accacgtcgc ctctcgagaa gatgtcggtt
    19561 ctttccgcct cttcaatggc gagctcgatc atctccttgt ccggcttctt ccagagatcg
    19621 tcggtgtcgt agcagaagta ctcgaagccc acccagacct tggccggatc ctggaccatg
    19681 aacggactcc agttgttgaa gatctgcata cggcccacca gcacgtcgtg ctcctgaatg
    19741 tagatccagt tgtccttcag cggttctgaa tttttccgcc ctttctctcc gagcctgagt
    19801 cgatccagca gaaggcccac cgtaataaag tcgcggtaaa tcaacccgtc gccgacttcc
    19861 ctgacttcgc gcggaatttc cacgtcaagg gcgtgaatca gctccttcac gggcatggtc
    19921 gagaaaacat gatccgcggg gaaatgccgc tcctcgccct gttcgttcac ggcggtgacg
    19981 ccggtgacac ggtcgcccga ggcatggata cgcgccacat tccatccggt gaggatctct
    20041 ccgccttccg cgcgaaccct gcgggacacc tcttcccaaa gttgtccggg accaaacttg
    20101 ggataaagaa acttctcgat gagagaggtc tcggtatcct tctgcgcgat atccttcgag
    20161 ccgcggccaa acagtttgcg cagatgatgg aggatcgccg ttcgaagaga caagcccttg
    20221 atgcgttgag ctccccactc ggcgctgatc tgatcgcagg gaacacccca taccttctcc
    20281 gtgtagctct tgaaaaacgt ccggtagagc tgccgtccga agcggttgat caaaaaatct
    20341 tcgagcgtct tctcgttcgg aataggccgg atggagctgc gcaaatagct gatgccggca
    20401 cggaacgttc tgaggagtcc cagttgtctc aacgtcaccg gagtgagctt gagcggatac
    20461 tcgaagagct tccggagaaa cagaatcctc gattgccttg gccgcaccag cagaacaaga
    20521 tcttccgatt tcggatcagg cccttttttt ccggttgcgg cgagtgtacg ctcacggttc
    20581 tgataaacca gccggtgatt ctcgtccgtc gtatcctcga cgggcattac ctccgtccac
    20641 cagttcatca cgcggtcgga ttttgaaaaa aaccgatgac ctccaatatc gatccggttt
    20701 cctttgtaat tgacggttcg tgatataccg cccatgtatc cgctcttttc gagcacaatg
    20761 ggcttgacct ttgcccgccg taagagctct agcgccgcag ttaaacccgc cggccccgct
    20821 ccaatgataa cggcggtccc ccgaagttcc ttcatgcaaa aatactccgc agtgtaaccg
    20881 agttctcgct gtccgtatcg gtttcgagtg agcttagtta ttattgtccg ccgctatctg
    20941 ccgcaccagg ctatgtgtac tgggacagcg gctcccggat caacgcctct tctaccaatg
    21001 tagaccagaa agcgaacacg cgaaagatat cggcaggtct atctgctttc gtgagggccg
    21061 cgggatgcgg actctgcgat acttcgtgta agctgatact attccgtacg cagagacggg
    21121 agtagtgatg ggtaagcgct atataacacg tcgtatggcg atatacgtcg cgctatttcc
    21181 gatctttcca gcgggctgcc gccgggcctt gaaggactcg gccagtctgg ttgagatggg
    21241 aaacctgtcc gtttcgaatc aattgcttag tggtttccat gacatcgaaa tggaacgctg
    21301 gcgctggaca gcacagagtt tttcggtcac cctgaagccg ccctccgcct ctgagcagaa
    21361 cggcgcgaca cttttattac gcctgtttat tcctgcagtc cagatcgaca agttgggacc
    21421 tattacgttg agttccgagg tggacgatca ggtactggat ccccaaacgt tttccaaacc
    21481 cggcgagtac acctatgcgc gcgatcttcc acccgcgttg cttgcaacga atgtacttcc
    21541 catccggttc tgcctggata gggcaacccc gggaacggag accgatggaa gggagctggg
    21601 aatcgtggtc acctcggtcg gcctgatatc gaagtagttc aaccgggcgc ctgacgggta
    21661 gtatttcagc cggcgcctgg cgcgtacggc gattattttg gcaaatcaga gaacaaagca
    21721 aaaacggatt tcacatgaaa acaatacttg taaccggagg agcaggtttt ttcggcggcg
    21781 tcttgaagcg ggaggttctt gccgcgggac atcgttgcgt cagtgtagat atctacccgg
    21841 atgaagacag tcaccgcaac cttatcacac accagttgga tatacgggac ggtgaagccc
    21901 tggaccgcct ctttgcatcg gagaaaatcg atggagtagt gcattgcgct gcaatactgg
    21961 cacatggttc ggaggatcac ggctttcttt ggagcagcaa tgtcgaaggc acgcgcagtg
    22021 ttgccctggc gatgcggaaa cacggcgtgc gccagttggt tttcacatcg acaaattgcc
    22081 tgtggggcga ggcgctgaag cggcccgttc gcgaagacga tacgccgaac ccgatcgaga
    22141 tttatggccg ctcgaagctg gaagcggaga aggtgctcgg cgaatttaca gatttgaacg
    22201 cggtgatcat tcgctgtccg accatcatcg acttcggacg cctgggcctg ctgtccattc
    22261 tgttcgagtt tatcgatgaa ggccgccggg tctggaccgt tggcggcgga cgaaaccggt
    22321 accagttcat ctacgcgaag gatctggcac aggcgtgcct gctggcgctg gattatccgg
    22381 aatccgaaac attcaatatt ggatcggacg acgtgaaatc tctcgccgag gtgtacgagt
    22441 atgtcatccg cagggctgga agtaagtcgt cggtgaggtc gctgcccagg ttcccggcga
    22501 tcttagccat gaaactggcc tacctgctga aaatctcccc tctgggaccg tatcactaca
    22561 agatgatcgc ggaggatttt ttgttcgata cctccaagat ccagcgtcta ctccattggc
    22621 gtccaactat gaccaacgag gagatgctct ggcgagcgta tgagtattat tccagaaacc
    22681 ggcgggaaat cgagaaccgc cgcaatgtct ccgcacatcg caaaggtgca cagatgggag
    22741 tgatccggct gcttaaatgg gtgtcatagc cgcctggccc cggagggcga tccgcacaga
    22801 tacactgcca caactggcgg tatattgtgc gggcgcccta tacatcctgg ttctgtttct
    22861 gactgtatcg catcacgagc cttgggccga tgaagcccag agctggctgc tggcgcgcga
    22921 tctgagtctt gctcaattgc tggcggtggc cccgcactat gaaggcacgc cgccgctctg
    22981 gcaccttctc cttcatctgc taatccgcct cgggttgcct tactccgccc tgaacatcgt
    23041 atcgggagca ttgggagtgg cggcggcgtg gcttatcgtg aggtatgcgc ccttaccgtt
    23101 gtggattcgg ctcgtcctgc cgtgtactta ctttctgggt tatcagtacc cggtgatcgc
    23161 ccgaa
//
LOCUS       DASTMK010000218        15812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_362324, whole genome shotgun sequence.
ACCESSION   DASTMK010000218 DASTMK010000000
VERSION     DASTMK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15812
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362324"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3120
                     /locus_tag="VFQ79_09745"
     CDS             <1..3120
                     /locus_tag="VFQ79_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139983.1"
                     /translation="DSIAEVHVEMNSSSAKFTRQTSIIMTTKSGTNDIHGSLFVTNRN
                     SGYGVARNRDNFTNTAAKLNRNEYGGTVGGPVWIPNVYNGKNRTFWFFSYEGYKYRSG
                     AFGNYRVPTEAMRNGDFSGLRDSSGTLQTIYDPLTTNAEFQRQPFNYGGRLNNIDPSR
                     ISPMMSYIYSVLPLPTLPNVNPLVGDNYSAPNPNIQDQYTWSARFDHRFSDNDLVYGR
                     ITNSMATTLRPAANGVPTLDGFGNSRTNTYPNKSLSVDWTRTFSPTFFNEVMFSASRT
                     VSTNFSGDPSRRYSTELGLPNPNGQPGYPVINNIGVGTGRSNYFQPVNWNMQYFNYFI
                     LDNNSTKIHGRHEFQFGVHLRHDQLNYMPQQQRTAGSVTFQANTTALYDPAASSATNR
                     RAVLNTGHVAAAAYLGYANYEVRAAKGMYYMRQNEDALYFQDNFRATNRLTLNLGLRW
                     QFSPYPKDKYNIFSSFDPKTMSIVLGQDLETLYRVGATTPALVRALEGYGAKFITAKE
                     AGLPTNLVKNNWFDIGPHVGFAYRAFDGPKSFVIRGGFSTSYFPVPIYGWNDRMRLNA
                     PFAGFYQNYALSQAAQSPDGLSNWGLVGTPTIVAGRNSSNVVTFDNPRGITVGEDSFQ
                     AAYFNPKQSSSRVHDWSLTVEKEIMADTVLRLAYLGNRASYQDSYDDWNAQMPEYVWL
                     STKGISPPSDSWASATTRPNPTLPYGNLQEYRRDGWGNSNGMTVELQRRYSKGLGFQV
                     FYQLVNAVKAAGHGWYSDSSVAPVSSFLPGTVPTDHHERMKLLLYTRDTTVPKHEIRF
                     NWIADLPFGKGKPVAGNAGSILNGFIGGWQVSGMGRWRSTYFNLPTDIWPTGDKVEYY
                     GHKYPIEDCRSGTCRPGYLLWNGYIPAHQINSVDANGKPNGVMGVPSNYKPAGQPLFP
                     YPADYNSRSAANDPNFPYYGSNYIWFNLSDTSTPYRFDLTGNDTGSPLHPWRNQFIRS
                     TNLWNADAAIFKAFPIKERVQFRIQFDFFNVFNTPGNDYLAGNDGLVGTWTNQNTART
                     MQASARLTF"
     gene            complement(3163..4329)
                     /locus_tag="VFQ79_09750"
     CDS             complement(3163..4329)
                     /locus_tag="VFQ79_09750"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0139984.1"
                     /translation="MSARIVIDIRHIKDFGIGTYIRNLLESIGKIDRENRYILVGREP
                     DFHYVSSLPPNFERIRYEHGDAHWFEQVRLPVFLRKLAPDLYHIPLNAVSILMPKPYV
                     VTIHDMGSLMYPTRKARASKFSLFRFRRGLQRAARVITVSGATRRDVEKLFSIPPERI
                     RQIYNAPDPRFLAKARSGHPSPSEAEMHDRERIRILGRYQIDYPFLLYAGTIRPQKNI
                     ARLVEAFAVVKGELEDHPDYKDLRLIIIGDEIAQYPSVRHAVIQTRVEQRVRFLGFVP
                     IDTLRIFYESAAAFVFPSLYEGFGLPPLEAMASGTPVVASSVSSLPEVVGDAAMIVNP
                     ENVFDIARGIKEVLLDMNLRERLIAKGYEQVGRFSWDHTAREVLATYKEVIEGK"
     gene            complement(4385..5668)
                     /gene="glyA"
                     /locus_tag="VFQ79_09755"
     CDS             complement(4385..5668)
                     /gene="glyA"
                     /locus_tag="VFQ79_09755"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU0139985.1"
                     /translation="MTEQERMSRPLADVDPEVYEAIRHETERQHSRLELIASENFTSE
                     AILEAAGSVFTNKYAEGYPGKRYYGGCEHVDVVENLARERAKALFKAEYANVQPHSGS
                     TANEAAYGAVLEPGDTILGMNLAHGGHLTHGHHLNFSGKRYKVVPYGVRKEDEIIDYD
                     ELARLADEHRPKMIIAGGSAYPRIIDFKRFREIADSVNALLLVDMAHFSGLVAAGVHP
                     NPCEYADIVTSTTHKTLRGPRAGLILASAQYGPAIDKSVFPGIQGGPLVHIIAAKAVC
                     FREADTPEFRSYAARAVTNARALARGLAAEGYRVVSGGTDTHLLLVDVFAKGIRGRDA
                     ETGLDKAHITANKNAIPFDTNPPLNPSGIRLGSPAVTTRGFGEQEMEQVARLIAETLA
                     NLDSEEVLASVRRKVAMLAERFPLYSWKLSPAAAR"
     gene            complement(5665..6111)
                     /gene="rpiB"
                     /locus_tag="VFQ79_09760"
     CDS             complement(5665..6111)
                     /gene="rpiB"
                     /locus_tag="VFQ79_09760"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEU0139986.1"
                     /translation="MKIAIAADHAGVSLKRRLREVLRRKGHEIVDLGTDSEESVDYPD
                     YARAVAVKVAEGAVDRGILICSTGVGMSIAANKVPGIRAALAVNPEEVRLTRAHNDAN
                     VLTIGAKFTPDSEAERMVDTFLATEFEAGRHARRLKKISELEHVSK"
     gene            6222..7928
                     /locus_tag="VFQ79_09765"
     CDS             6222..7928
                     /locus_tag="VFQ79_09765"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU0139987.1"
                     /translation="MQWSKLFIPTLRENPAEAEVVSHQLLLRAGYIRQLAAGLYSYLF
                     LAQRSILKITNIVREEMDAIGAQEMLLPALNPAEIWQESGRWDIMGANMFRLKDRWQR
                     DLCLGMTHEEIMTSIARGELRSYKHLPQIWYQIQTKFRDEPRPKSGLLRVRQFLMKDS
                     YSFDMDAAGLDAAYQKHYAAYHRIFDRCGLKYTVVEAHSGAMGGSQSHEFMVSSDAGE
                     DFVVLCRETGYAANLEKAVSRPVPPAAADPEGDLAPEEFHTPGRKTIAEVAEFTNLPE
                     TSQMKSLVMVGDGKPVLALLRGDHTLSETKFASVTGAADIRPAHPEEIREWFGADAGS
                     LGPVGVRNLRILADSALEGRRNMIAGANRDDYHLRHVTPGEDFTPEYCDLRQVTAGDT
                     EITTGAPLEIAKSVEIGHIFKLGYKYSESMGLRVLNEAGQEVTPIMGSYGIGMERILC
                     AAIELYHDKDGMSLPSSIAPFTVVITPVNWADEKQRAAAGDLYRKAKLLGLDALLDDR
                     DERPGVKFKDADLIGVPYRITLGKKLAQGIAEVVERRTKTSYDVPLEGAAQFVADKVH
                     AG"
     gene            7918..9036
                     /locus_tag="VFQ79_09770"
     CDS             7918..9036
                     /locus_tag="VFQ79_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0139988.1"
                     /translation="MPVDWAAIRSEFPALQNWTFLNTATFGQLPRRATEAVNRHFFHR
                     DKMACWDFLDWFDDADRIRESVARLIHCEAGDIAFIQNAASALSLLIDGIEWKEGDRI
                     VTLRDEFPNNLYHPSVLETKGVQCVEVDWSEFYQAIDERTRLVAMSTVNYTSGLRPAA
                     EEVSRYLRERGVVLYLDGTQSVGALEFDTARIRPDVLAVHGYKWLLSPNGAGFMYVAP
                     ELRERLQPSVIGWRSHKDWRNVDNLHHGAPEFKHEAEKYEGGMLPFPLLYAMEASVQM
                     MLEIGPAEIENRVLGLTAQLRDSLRAMGASVLDGRDYCSPITAARFDNVDASRLAREL
                     KQRRVLVSARHGNVRISVHFYNNEADLERFSRELQALL"
     gene            complement(9028..9600)
                     /locus_tag="VFQ79_09775"
     CDS             complement(9028..9600)
                     /locus_tag="VFQ79_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU0139989.1"
                     /translation="MTREEAWDILCEFTRSENLRKHALAVECCVVAYARRYAEDVEKW
                     SVTALLHDFDWEIHPEAPDHPMKGEPILAARGVSEEIRRAILSHANYSGVPRESLLEK
                     TLFACDELAGFLTAVSLVKPGRSIFEVDVKSVRKKMKDKAFARSVSREDIVNGAAGLG
                     VGLDEHIDFCIQAMQGRAEELGLKGTAGSQ"
     gene            complement(9610..9831)
                     /locus_tag="VFQ79_09780"
     CDS             complement(9610..9831)
                     /locus_tag="VFQ79_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0139990.1"
                     /translation="MRNIFEIANFLIERPALTGAVVWGVLYAVRRSKRHKLSEKEREH
                     ALQVHSEQRSYAEKMYDPLQETETGAKPR"
     gene            complement(9915..11879)
                     /locus_tag="VFQ79_09785"
     CDS             complement(9915..11879)
                     /locus_tag="VFQ79_09785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0139991.1"
                     /translation="MRRLAVLLVISPLVCLAQTPTFWTPEVSMQVKGIGSVVPSNDGT
                     LAAYEQSIQVMETERSETLTHIYLAKSDGSDRIQLTRGEKNCTSPSFSPDGQYVYFKS
                     SRTGAGNLWRIAVRGGEAEQLTDWKGEIGSYEVSPDGKLVAFTAREKDPDEEAAGKEK
                     RDFRVVDEKPKNHGLWVIEAESAAAEKRAPRAIVKANYHIAGFAWSPDSRFLAFAHVP
                     TPNADDWPKSDLSEVDVVSGAVKTLAATAAAESDPSYSPDGRHIAFERSSIPPTWQYD
                     TRIVVLARDSGASRELPATFDQNPVLAGWSSDSKSLLVREPKGTRAALYWIPLDGPVK
                     TLYVPERGIIGPPRLNQTGTHMGVAMESSGVPPEAFVIALNEWKPVRVSGANVDLPKY
                     PFGDTAVIAWKSKDGMPVEGLLTYPVGFEKGRKYPLILNIHGGPAGVFGESFIGRPGA
                     YPLATFAAKGYAILRPNPRGSSGYGKKFRFANLSDWGYGDYEDLMAGVDHVVTMGVAD
                     PDRLAVMGWSYGGFMTSWVVGHTDRFKAAVVGAGVTDLLSFTGTADIPGFLPDYFAGE
                     PWKNPEAYRKHSPVTYVGNVSTPTLVLHGEADERVPVTQGYQFYSGLKRRGVATRMVV
                     YPRTPHGVREPKFMLDVMQRHLDWLERYIK"
     gene            12076..12834
                     /locus_tag="VFQ79_09790"
     CDS             12076..12834
                     /locus_tag="VFQ79_09790"
                     /inference="COORDINATES: protein motif:HMM:NF009385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0139992.1"
                     /translation="MANELKGKVAIVTGATRNQGRAYAEMLAANGCANVVVHHRGENS
                     RSEAEETANAVRAKGAEPLVFQADLTKVSEVRRMFDESDKRFGRLDILINNAGVVIKK
                     PFTEITEEEYDRAFGINAKAAFFCMQEAARRISDGGRIINIGTSLLGATTGSYSVYAG
                     SKAPLEDFTRALAKEIGQRGVTVNTVCPGPLNTSFFYPAETPDSIEFFKHMSVQNRLG
                     EIGDVVPLIEFLVSPKGGWVTAQTIFVNGGFLAR"
     gene            complement(12884..14212)
                     /locus_tag="VFQ79_09795"
     CDS             complement(12884..14212)
                     /locus_tag="VFQ79_09795"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEU0139993.1"
                     /translation="MTADTTIHSSAPLPDLETLDRSNSGWLSHSRRVAVLAMEVGTAL
                     IEPAHRELLAQAALLHHYPAVFLKPDAWSRMLADLYGPEWHSLIGYEHSPEFPPAMVA
                     EVLRALHSRRTGKAASPIERIADLLEVCCLFDERLEFLPFEDTNLKEILAEFDLMASS
                     GVFDVATVRALHRFPLASLEQVNRLMGHLPVFPAVAVRVLATFRSSNSEVSPSRLEAL
                     AGNDQVLAGEIIRAANSCVYGVSRTISRLGEAIVYVGSETARNLLIAAAMKPLFASAA
                     MRELWRHSLDVAQLAHTLAVKSDTADRDEAFLAGLVHDAGRLLLSLLPREIAELHARV
                     TEQGCPALMADLLVSGLDHAELSAMVLKKWKFPEHLVQAAGLHHKPELSGESLPSILY
                     LAEILSGSKEDIHAGTRIDAAMTRLHLAAQDLVDLSDRTSPAIDAIRGAA"
     gene            14443..>15812
                     /locus_tag="VFQ79_09800"
     CDS             14443..>15812
                     /locus_tag="VFQ79_09800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEU0139994.1"
                     /translation="MADFPETPQPPTPPVPPSGGGGQIPLGGDGNLIPINIEDEMRRS
                     YLDYAMSVIVGRALPDIRDGLKPVHRRVLYGMHEMGLHYNRPTRKCAKITGEVMGKYH
                     PHGDASIYDTLVRMAQPFSMRYPLVDGQGNFGSVDGDPPAAMRYTEARLSRIAGTLLE
                     DIDKETVDFRPNYDESEVEPEVLPTRVPNLLINGSSGIAVGMATNIPPHNLTEVVNAT
                     IALIRNPKTPLLEILQMVPGPDFPTGGFILGRQGIFDAFTKGRGSLKLRARAAIEHVT
                     KDRDAIIVTELPYQVNKARLVADIAAQVNDKKIEGISDVRDESDRDGMRIVVELKRGE
                     QAEVILNNLYKHTQMQVNFGVIMLSIVNGQPRELGLIDCIKRFIDHRIDVVRRRTDYL
                     LRKAREREHLLLGFKKALENLDAVIELIRASRNPREARESLVTRFEFTERQAQAIIEL
                     QLQRLTG"
BASE COUNT         3336 a         4818 c         4570 g         3088 t
ORIGIN      
        1 gacagtatcg cggaagttca cgtcgagatg aactcctctt ccgcgaaatt cacgcggcag
       61 acgagcatta tcatgacgac gaagagcggc acaaacgata ttcacggatc gctctttgtc
      121 accaaccgga acagcggcta cggagtggcg cggaaccggg acaacttcac caacaccgcg
      181 gcgaagctca accgcaacga gtatggcggc acggtaggcg gcccggtgtg gattccgaac
      241 gtctataacg gcaagaaccg gaccttctgg ttcttctcct acgaaggcta caagtaccgc
      301 agcggcgctt tcgggaacta ccgggttccc acggaggcga tgcgcaacgg agatttttcc
      361 ggcttgcgcg actcttccgg gacgctccag accatctacg atccgcttac gacgaatgca
      421 gaattccagc ggcagccgtt caattatggc ggcaggctga acaatatcga cccgtcgcgg
      481 atcagcccga tgatgagcta tatctatagc gttctgccgt tgcctaccct ccccaatgtg
      541 aatccgctgg tgggcgacaa ctattcagcc ccgaacccga acattcagga ccagtacacc
      601 tggtcggcgc gtttcgatca tcgtttctcg gataacgacc tggtgtacgg ccgcatcacc
      661 aacagcatgg ccaccacgct gcgtcctgcc gccaacggtg ttccaacgct cgatggattc
      721 ggcaactcgc gcacgaacac gtatccgaac aagagtctgt cggtggactg gacgagaacg
      781 ttttcgccga ccttcttcaa tgaggtgatg ttcagcgcca gcagaacggt gtccaccaac
      841 ttctccggcg atcccagccg ccgctactcg acagagttgg gcctgcccaa cccgaacgga
      901 caaccgggtt acccggtgat caataacatc ggggtgggca ccggccgcag caactacttc
      961 cagccggtga attggaacat gcagtacttc aattacttca tcctggacaa caattcgacc
     1021 aagatccacg gccggcatga gtttcagttc ggggtgcacc tgcggcacga ccagttgaac
     1081 tatatgccgc agcagcagcg caccgccggt tcggtcacct tccaggcgaa tacgaccgcg
     1141 ctttacgatc ccgccgccag cagcgccacg aaccgccggg cagttctgaa taccgggcac
     1201 gtggcggccg ccgcgtatct cggatacgcc aattatgaag ttcgagctgc caagggcatg
     1261 tactacatgc ggcaaaacga agatgcccta tacttccagg acaacttccg cgccaccaac
     1321 cggttgacgt tgaacctcgg cctgaggtgg caattcagtc cctatccgaa ggataaatac
     1381 aacatcttct cgagcttcga tcccaagacg atgtccatcg tgctcggcca ggatctggag
     1441 acgctatacc gcgtgggcgc gaccacgccg gcgctggtta gggctctgga aggctacgga
     1501 gcaaagttca tcaccgcgaa agaagcgggc ctgccgacga acctggtaaa gaataactgg
     1561 ttcgatatcg gccctcacgt cggctttgca taccgcgcgt ttgacggccc caagagcttc
     1621 gtgattcgag gcggatttag caccagctac ttcccggttc ccatctacgg ctggaacgac
     1681 cgcatgcggc tgaatgctcc gttcgccggg ttctatcaga actacgcgct ctcgcaggct
     1741 gcccagtcgc cagatggcct gtcgaactgg ggactcgtgg gaactcccac gatcgtcgcc
     1801 ggcaggaaca gttccaacgt tgtgaccttc gataatccgc gtggcatcac ggtcggcgag
     1861 gattccttcc aggcagccta cttcaacccg aagcagtcca gttcccgcgt acatgactgg
     1921 agtctcacgg tagaaaaaga gatcatggcg gacaccgttc tgcggcttgc ttatctcggc
     1981 aaccgcgcct cgtaccagga cagctacgac gactggaatg cgcagatgcc agaatatgtc
     2041 tggctgagca caaaggggat ctctcccccc agcgattcct gggccagcgc caccacgcgc
     2101 ccgaatccaa cgctgcccta cggcaacctt caggaatacc gcagagacgg atggggcaac
     2161 tcgaatggta tgacggtcga gttgcagcgc cgctattcga agggcctcgg cttccaggtg
     2221 ttctatcagc tcgtgaacgc cgtcaaagcg gcgggccatg gctggtacag cgactcttcc
     2281 gtggcgccgg tcagttcatt ccttccgggc acggtgccga cggaccacca cgagcgcatg
     2341 aaactgcttc tctacacccg cgataccacg gtgccgaagc acgagatccg cttcaactgg
     2401 attgccgacc tgccgttcgg caagggcaaa ccggttgccg gtaacgccgg ctccatcctg
     2461 aacggcttca ttggcggctg gcaggtgagc ggtatgggcc gctggagaag tacctacttc
     2521 aatctgccca ccgacatctg gcctactggc gacaaagtcg agtactacgg gcataagtat
     2581 ccgatcgaag actgccgcag cggcacctgc cgtcccggct acctgctgtg gaacggctac
     2641 attccggcgc accagatcaa cagcgtggat gcaaatggaa aaccgaatgg agtgatgggt
     2701 gtccccagca actacaagcc ggcgggtcaa cccttgttcc cctatcccgc cgactacaac
     2761 agccggagcg cggcgaacga cccaaacttc ccctactacg gatccaatta catctggttc
     2821 aatctatccg ataccagcac accgtaccgg tttgatttga ccgggaatga taccggaagt
     2881 ccgctgcatc cctggagaaa ccagttcatt cgcagcacaa acctttggaa cgccgatgca
     2941 gccatcttta aggcgtttcc gattaaagaa agggtacaat tccgtattca gttcgacttc
     3001 ttcaacgtgt tcaacacgcc cggcaacgac tatcttgctg gaaacgacgg cctggtcggc
     3061 acctggacga atcagaacac ggcacgcacc atgcaagcgt ctgcacgctt gaccttctaa
     3121 ttacggttca cggcggcagg gcgcaagctc tgccgccgct tttcacttcc cctcgatcac
     3181 ttctttatac gttgccagaa cttcccgtgc cgtgtgatcc caactgaagc ggcctacctg
     3241 ctcatagcct ttcgcgatga gcctttcccg caaattcata tcgagcagga cctctttgat
     3301 gccgcgcgcg atgtcaaaga cgttctccgg gttgacgatc atcgcggcgt cccccaccac
     3361 ctcgggcagt gaactgacgc tggaggcgac caccggggtt cccgatgcca tcgcttcgag
     3421 cggcggaagt ccaaacccct cgtaaagcga cggaaaaaca aacgccgcgg ccgattcata
     3481 aaaaatccgc agcgtgtcga tagggacgaa cccgaggaac cggacccgct gctccacacg
     3541 cgtttggatc acggcgtggc gcacggaggg gtattgcgca atctcgtccc cgataatgat
     3601 gaggcgaaga tccttgtagt ccggatgatc ctccagttcg cccttcacca ccgcgaacgc
     3661 ctccaccagc ctggcgatat tcttctgcgg ccggatggtc ccggcataca gcagaaacgg
     3721 gtagtcgatc tgatagcgtc ccagaatccg tatgcgttcg cggtcgtgca tctcggcctc
     3781 ggacgggctc gggtggccgg acctggcctt cgccaaaaac ctcgggtcgg gggcgttgta
     3841 gatctggcgt atgcgttcag gcggtatgga gaacagcttt tcgacatctc tccgcgtggc
     3901 gcctgaaacg gtaatgacgc gcgcggcgcg ctgcagcccc ctgcgaaacc ggaagagcga
     3961 aaacttgctc gcccgggctt ttcgcgtcgg atacatcagg ctgcccatat cgtgaatggt
     4021 gacgacgtat ggcttgggca tcagaatcga aacggcattc agcgggatgt gatacaagtc
     4081 cggcgcgagc tttcgaagga acaccggtaa ccggacctgc tcgaaccagt gcgcgtctcc
     4141 gtgttcgtaa cgaatgcgct cgaagttcgg gggcagcgat gagacataat ggaaatccgg
     4201 ctcccggcca accaggatgt aacggttctc acgatcgatt ttgccgatcg actcaagcag
     4261 gttccggatg tacgtgccga ttccgaaatc tttgatgtgc cggatgtcga tgacaattcg
     4321 agcggacatg atatagcaaa agggccgacc ggggacggcc acactccgga ttttccggcg
     4381 cgggttaccg cgcggcggcg gggctcagtt tccagctata cagcgggaag cgttccgcca
     4441 gcatggcgac ttttcgccgc accgaggcca gaacctcttc ggaatcgaga ttcgccaagg
     4501 tttcggcgat gagccgggcc acctgttcca tctcctgttc cccgaatccg cgcgtggtca
     4561 ccgccgggct gcccagccgg atcccactcg gattcagcgg aggattcgta tcgaacggga
     4621 tcgcgttctt attggctgtg atgtgcgcct tatccagacc ggtctccgca tctctgccgc
     4681 ggatgccctt ggcgaacacg tccaccagca gcaggtgggt atcggtgccg ccggatacca
     4741 cgcggtagcc ttccgccgcc agcccgcgcg ccagcgcccg cgcattcgtg acggcacgcg
     4801 cggcgtacga gcggaattcg ggtgtatccg cctcacggaa acaaaccgct tttgccgcga
     4861 tgatgtgcac cagcggtcct ccctgtatgc cgggaaagac actcttgtcg atcgcgggac
     4921 catattgcgc gctggcaagg atcagtccgg cacgcgggcc gcgcagggtc ttatgcgtcg
     4981 tagacgtcac gatatcggcg tattcgcagg ggttgggatg cacccccgcg gccaccaggc
     5041 cggagaaatg ggccatgtct accagcagca gggcgttcac cgaatcggcg atttcacgaa
     5101 aacgcttaaa gtcgataatt ctcgggtacg cgctgccacc cgcaatgatc atctttggac
     5161 ggtgctcatc tgcaagccgc gcgagttcgt cataatcgat gatctcatcc tcttttcgca
     5221 cgccgtacgg caccaccttg taccgcttac cggaaaaatt cagatggtgg ccgtgcgtca
     5281 gatggccgcc gtgtgccagg ttcatcccga ggatggtgtc gcctggttcc agcacggcgc
     5341 cgtaggcggc ttcattggcg gttgaaccgg aatgcggctg aacgttggcg tactccgctt
     5401 tgaaaagggc tttggccctt tcacgggcga gattctccac gacatcgacg tgctcgcagc
     5461 cgccgtaata gcgctttccg ggatagccct ccgcgtactt gttcgtgaaa acgctgcccg
     5521 cggcttcgag aatcgcttcc gaagtaaagt tctcgctggc aatcagttcc agccgcgaat
     5581 gctgccgctc cgtttcatgg cgaatcgcct cgtacacttc cgggtccacg tccgccaggg
     5641 gccgtgacat tcgttcctgt tcagtcattt cgatacgtgc tccaactccg agatcttctt
     5701 gagacggcgt gcgtggcggc ctgcttcaaa ctcagtcgcc aggaaggtat ccaccatccg
     5761 ttccgcttcg ctgtccggtg taaatttcgc gccaatggtg agcacattcg catcgttatg
     5821 agcccgggtc aggcgtactt cctcgggatt taccgcaagc gccgcacgga taccaggcac
     5881 tttattggct gcgatggaca tgcccacgcc agtactgcaa atcagaattc cacgatccac
     5941 cgcgccttca gcgaccttga ccgcaacggc gcgagcgtaa tccggatagt cgacggattc
     6001 ttcggaatcg gtgcccagat ccacgatttc atgacctttc cgccggagta cttcccgcag
     6061 ccgccgcttc agggacaccc cggcgtgatc cgcggcaatg gcgatcttca ttggggttaa
     6121 tggatcaatt ctaacatgct agaattgaaa tctcgttccg ccggggaatc gcagtgccgc
     6181 tacattgcgg cgcgtcttcc tttcacaaac gagcaacatc catgcagtgg agcaaactgt
     6241 ttattcctac tctgcgggaa aatcccgcgg aagccgaagt tgtaagccat caattactgc
     6301 tgcgcgccgg atacattcgc cagcttgccg ccggactcta cagctatctg tttctcgcac
     6361 aacgatcgat tcttaaaatc acgaacatcg ttcgcgaaga gatggacgcc attggagcgc
     6421 aggagatgct tttacccgcg ctaaacccgg cggaaatctg gcaggaatcc gggcgctggg
     6481 acatcatggg cgcgaacatg ttccggctga aagaccgctg gcagcgggat ctctgtctgg
     6541 gcatgacaca cgaagagatc atgacctcca tcgcgcgcgg agagctccgg agttacaagc
     6601 accttccgca gatctggtat cagatccaga cgaaatttcg cgacgaaccc aggcctaaat
     6661 cggggctgct gcgggtgcgg caatttctca tgaaggattc ctacagcttc gatatggatg
     6721 ccgccggtct cgacgcggcc tatcagaagc actatgccgc ctatcaccgc atctttgacc
     6781 ggtgcggatt gaaatacaca gtcgtggaag cgcactccgg ggcaatgggg ggcagccaat
     6841 cccacgaatt catggtgtct tcagacgccg gcgaggattt cgttgtgctg tgccgggaaa
     6901 ccggttatgc ggctaacctg gaaaaagccg tatcgcgccc ggttccaccc gccgcggcgg
     6961 acccagaagg cgatctcgct cccgaggagt tccatactcc cggcagaaaa acgattgccg
     7021 aagtcgccga gttcacgaac ctgcccgaga catcgcaaat gaagagcctg gtgatggtgg
     7081 gcgatggcaa gcccgttctg gcgctgctgc gcggcgatca tacactcagc gaaaccaagt
     7141 tcgccagtgt caccggcgct gccgatattc gtcccgctca tccggaagag atccgcgagt
     7201 ggttcggcgc cgatgcgggt tctctcggac ccgtcggcgt ccgcaatcta cgtatcctcg
     7261 cggattccgc gctcgaaggg aggcgcaaca tgatcgcggg agccaatcgc gacgattacc
     7321 atttgcgcca cgtcacaccg ggtgaggatt tcacgcccga gtattgcgat ctgcgccagg
     7381 tcacggccgg cgataccgag atcactacag gcgcaccgct cgaaatcgca aagagcgtcg
     7441 agatcggcca catcttcaag ctcggttaca aatattcgga atcgatggga ctgcgtgtgc
     7501 tcaacgaagc cggtcaggaa gtaacgccca tcatgggttc ctatgggatc ggcatggaac
     7561 gaattctctg tgcggccatc gagctctacc acgacaaaga cggcatgtcg ctgccgtctt
     7621 ccatcgcacc attcaccgtg gtgatcacgc cggtaaactg ggccgatgag aagcagcgtg
     7681 ccgccgccgg cgatctctat cgcaaagcaa agttgctggg actggacgcc ctcctggacg
     7741 accgggacga gcgccccggt gtcaagttca aggacgcgga tctgatcggc gttccctatc
     7801 gcatcacgct tggcaagaag ctggcgcaag gcatcgcgga agtggtggaa cggcgtacca
     7861 aaacaagtta cgacgtaccg ttggaaggcg cggctcagtt cgtcgcggat aaagtccatg
     7921 ccggttgact gggccgcgat ccgatcggag ttccccgccc tccagaactg gacgttcctg
     7981 aacaccgcga cattcgggca actcccgcgc agggctacgg aagctgtgaa ccggcacttc
     8041 ttccaccggg ataagatggc gtgctgggac ttcctcgatt ggttcgacga tgcggaccgg
     8101 atccgggaaa gcgttgcccg cctgattcat tgcgaggccg gggatatcgc ttttatacag
     8161 aacgcggctt cagcgctctc gttgctgatc gacggtatcg agtggaaaga gggcgaccgc
     8221 attgtgacac tgcgggacga gtttccgaac aacctctatc acccgtcggt tctggaaacc
     8281 aaaggcgtcc aatgcgtgga agtggattgg agcgaattct accaggcgat agatgagcgc
     8341 acgcggctgg tggcgatgag caccgtcaac tacaccagcg ggctgcgccc ggcggcggaa
     8401 gaggtatcac gctatctgcg cgaacgcggc gtggtgctct atctggacgg cacgcaaagc
     8461 gtcggcgcgc tggagttcga taccgctcgt atccgtcccg acgtgctcgc cgttcatggc
     8521 tacaagtggc tcctgtcacc caatggagcc ggctttatgt acgttgcacc ggagcttcgg
     8581 gagagactgc aaccctcggt gatcggctgg cgcagccata aggactggcg taacgtggac
     8641 aatctacacc acggcgcacc cgaattcaaa cacgaggcgg agaagtacga gggtggaatg
     8701 ctccccttcc cgctgctcta cgcgatggaa gcgtccgtgc agatgatgct cgaaattgga
     8761 cccgcggaga tcgaaaacag agtgctcggt ttaacggcac aactgcgcga ttcgcttcgg
     8821 gcgatgggcg cctcggttct ggatggacgc gactattgct cacccatcac cgccgcccgg
     8881 ttcgacaacg tggacgcttc ccgcctcgcg cgggaactga agcagcggcg tgtgctggtc
     8941 tccgcacgcc atggcaacgt caggatttcc gtccacttct acaacaacga agcggacctg
     9001 gagcggttca gccgggagtt gcaggcgcta ttgtgagccg gcagttccct tgagccccag
     9061 ctcctccgcc cgtccctgca tcgcctgaat acagaagtcg atatgctcgt cgagtccgac
     9121 acccaatccg gccgcgccgt tcacaatgtc ctcgcggctg accgagcgtg cgaacgcttt
     9181 gtctttcatc ttcttccgca ccgatttcac atccacctcg aagatgctgc ggccgggctt
     9241 tacaagggaa acggcggtga gaaagcccgc cagttcgtcg cacgcaaaca gcgttttctc
     9301 gagcagggat tcgcggggca ctccgctgta gttcgcgtga gataaaatag cgcgccgaat
     9361 ctcctcgctg acgccccgcg cggccaggat cggttcccct ttcatcggat gatccggcgc
     9421 ttccggatgg atctcccaat cgaaatcgtg aagcaatgcg gtgacggacc acttctcgac
     9481 atcctccgca tagcggcggg cataagccac gacacagcac tccaccgcta aggcgtgttt
     9541 acggagattt tcggacctgg taaattcgca caggatgtcc catgcttctt cacgcgtcat
     9601 ggcatcgcat cagcggggtt tcgcgccggt ttcggtctcc tgcaggggat cgtacatctt
     9661 ctccgcatag gagcgctgct cgctgtggac ctgaagcgca tgctctcttt ccttctccga
     9721 aagcttgtgc ctcttggacc ggcgaaccgc atacaacacg ccccacacca ccgcgcccgt
     9781 caatgcgggc cgttcaatca ggaagttagc aatctcgaaa atgttgcgca taattcgagc
     9841 cgaaaggccc gacgcacttc atcttagcag ttcgtgagcg aaccagaaac cggtcattgc
     9901 ccggcgggaa atgcttactt tatgtagcgc tccaaccagt ccagatggcg ctgcatgaca
     9961 tcaagcatga atttcggctc gcgcactcca tgcggtgttc gcggatacac caccatgcgg
    10021 gtggccacgc cgcgccgctt caatccgctg tagaactgat aaccctgcgt gaccggaacg
    10081 cgttcgtccg cctccccgtg cagcaccagc gtgggcgtgg acacattccc cacgtaggta
    10141 accggcgagt gcttccggta agcctcgggg ttcttccacg gctcaccggc gaaatagtcc
    10201 ggcaggaacc cggggatgtc tgccgtgccg gtgaagctga gcaggtccgt gacaccggcg
    10261 cctacgacag cagccttgaa ccggtccgtg tggccgacga cccaactggt cataaatccg
    10321 ccgtaactcc aacccatcac cgccaggcgg tccggatcgg caacgcccat ggtgacgaca
    10381 tgatccaccc cggccatcag gtcctcatag tcgccgtagc cccagtccga caggttggca
    10441 aaccggaact tctttccata accgctggag ccgcgcgggt tcggccgcag gatcgcatat
    10501 cccttggccg cgaaggttgc cagcggatac gcgccgggcc gtccgataaa cgattcgcca
    10561 aagacgccgg ccggtccgcc gtggatattc agaatcaagg ggtacttcct gcctttttcg
    10621 aaacccaccg ggtatgtcag cagaccttcc accggcatgc cgtccttcga cttccaggcg
    10681 atgaccgccg tatcgccgaa cggatacttc ggcagatcca cgttggcccc gctcacccgc
    10741 acgggcttcc attcattgag cgcgatgaca aacgcctcgg gcggcacacc ggacgactcc
    10801 attgcgacgc ccatatgagt gccggtttga ttgagccgcg gcgggccgat gattccccgt
    10861 tccggcacat agagagtctt caccggacca tccagaggaa tccagtagag agcggcccgt
    10921 gtgcctttcg gctccctgac cagcagactc ttcgaatcgg aactccagcc ggcaagaaca
    10981 ggattctggt cgaaagtggc ggggagctct cgcgaggcgc cgctgtcgcg cgccagcacc
    11041 acgatcctcg tgtcatactg ccacgtcggc ggaatcgagg agcgctcaaa tgcgatgtgc
    11101 cgtccatccg gcgaataact cggatcgctt tcggcagccg ccgtcgcggc gagcgttttc
    11161 acggcgcccg aaaccacgtc cacctcggac aaatccgatt tcggccaatc gtcggcgttt
    11221 ggagtgggta cgtgggcgaa ggcgaggaag cgtgagtcag gcgaccaggc gaatccggca
    11281 atatggtagt tcgccttgac gatcgcgcga ggggcacgct tctccgctgc ggcgctctct
    11341 gcttcaatca cccagagccc gtggttcttc ggcttctcgt cgacaacgcg gaagtcgcgc
    11401 ttctccttgc ctgccgcctc ctcgtcgggg tccttctcgc gtgctgtaaa agccaccagt
    11461 ttcccgtcgg gcgaaacttc gtacgagccg atttctcctt tccaatcggt caactgctcc
    11521 gcctctccac cccgtacggc gatgcgccag agattaccgg cacccgtgcg tgacgatttg
    11581 aaatacacgt actgtccgtc cggcgagaaa gaaggtgacg tgcagttttt ttctccgcgc
    11641 gtaagctgaa tacgatcgga gccgtcggac ttcgcaaggt agatgtgtgt gagcgtttcg
    11701 ctgcgctctg tttccatcac ttgtatggat tgctcgtaag cggccagcgt cccgtcattc
    11761 gaaggaacaa cggacccgat gcccttgacc tgcatgctta cttcaggcgt ccagaacgtg
    11821 ggcgtttggg cgaggcaaac cagcggggaa atgacaagta acacagccag gcgtcgcatg
    11881 tcggatagtg tactccaatg cgcggtggcc gggaggagac gatgtttctg tcgtgaggca
    11941 taatcgggga tatgtctggc tgggcgcgta ttcacgcgag ttcaatggtc cgtatggtgc
    12001 aggaggcttt aagttgtagg cgctgcgcgg acagagcacg cgacagccga atccatgcaa
    12061 tcagggagag atcacgtggc gaatgaactg aaaggtaaag ttgcgatcgt aaccggagcg
    12121 acccggaacc aggggcgcgc ctacgcggaa atgctcgccg caaacggctg cgccaatgtc
    12181 gtcgtccacc accggggaga aaattcgcga tccgaagcgg aagagacggc caacgcggtc
    12241 cgtgctaaag gcgctgaacc gctcgtattt caagctgatc taacgaaggt gagcgaagtg
    12301 cgccggatgt tcgatgagag cgacaaacgc ttcggccgtc tcgatatcct cattaacaac
    12361 gcgggcgtcg taatcaagaa gcccttcact gagatcacgg aagaggaata cgatcgtgcc
    12421 ttcgggatca acgccaaagc cgccttcttc tgcatgcagg aggcggcacg gcgcatttcc
    12481 gacggcggcc gcatcatcaa cattggcaca agcctgcttg gcgccacgac tggttcctat
    12541 tccgtctacg ccggcagcaa agcgccactg gaggacttca cccgcgcgct ggccaaagaa
    12601 atcgggcaac gcggagtcac cgtgaataca gtatgccccg gccctcttaa cacatctttc
    12661 ttttatcccg cggaaacgcc ggattcgatc gaatttttca agcacatgag tgtacagaac
    12721 cggctcgggg agatagggga cgtagtgccg ctcatcgaat tcctggtttc cccgaaaggc
    12781 ggatgggtaa cggcacagac catattcgta aacggtggct tcctcgcacg ctgaaatgag
    12841 gccaggcgcc gttgcaggtc accgaagagg gtccggccca gctttatgcg gcgccgcgaa
    12901 tggcatcgat agcagggctg gtccgatcgg acaaatcgac caggtcttgc gccgcaagat
    12961 gcaggcgcgt catagcggcg tcgatccgtg tcccggcgtg tatgtcttct ttcgatccgc
    13021 tgaggatttc cgccagatac aagatagacg gcagggactc acccgataac tccggtttat
    13081 gatgcaaccc ggcagcttgc accagatgtt ccgggaattt ccactttttc aggaccatcg
    13141 cgctgagttc ggcgtgatcc aggcctgata ccaacaggtc cgccatcagc gccgggcagc
    13201 cctgttcggt gactctggcg tgaagctccg cgatctccct gggtagcagc gaaagtagca
    13261 gccgcccggc gtcgtgcacc aatcccgcca gaaacgcttc atcccggtct gccgtgtcgg
    13321 atttcacggc cagcgtgtgg gccaactggg ctacgtcgag agaatgccgc cagagctcgc
    13381 gcatggcagc ggaggcaaac aggggtttca tggcagccgc aatcagcagg ttgcgcgcgg
    13441 tctcggaacc gacgtatacg atagcctcgc ctaacctgga gatcgtgcgg gatacgccgt
    13501 agacacagga attggcggca cggatgatct cacccgccag gacctggtcg ttgcctgcca
    13561 aagcctccaa ccgggacggg ctcacctcgg agttggaaga acgaaacgta gccaggactc
    13621 tgacggcaac ggccgggaaa accggcagat ggcccatcag acggttcacc tgctccaggg
    13681 atgccaacgg gaagcggtgg agggcccgta ccgtcgcgac atcgaatacg ccgctggagg
    13741 ccatcaaatc gaattcagcg agaatctcct ttaagtttgt atcttcaaat ggcagaaact
    13801 caaggcgttc gtcgaacaga caacagactt ccagtagatc ggcgatcctc tcgatcggac
    13861 tggctgcttt tcctgtccgg cggctgtgca gcgcccggag gacttccgcc accatcgcgg
    13921 gaggaaactc ggggctgtgt tcgtagccta tcagggagtg ccattcaggc ccgtaaagat
    13981 cggccagcat ccggctccag gcgtcgggtt tgaggaacac ggcggggtaa tggtgcagca
    14041 gcgccgcctg cgccagcaat tcgcggtgcg ccggttcgat aagagcagtc ccgacctcca
    14101 tcgccagcac cgccaccctg cggctatgcg atagccagcc gctattcgac ctgtcaaggg
    14161 tttcgaggtc cggcaggggt gcggagctgt ggatagtagt gtcggcggtc attgtttccg
    14221 ttcccatctg gccttatcgg tatgagagat gtttgtatta gcccggtaca aagaaagttc
    14281 cgggagggtc tggcagacag agggaagccg tccaaaagac cccaattttt caatggtttt
    14341 tggtaaaatt agagttacac ctcgcaggcc ccttctggcg acgatgatgc tccggcgaac
    14401 tgtgccggaa gctcctgccg ggccacaaaa aaggagtcta ttttggctga ttttccggaa
    14461 accccgcaac cgccaacgcc cccggttcca ccttcgggtg gaggcgggca aatcccgctg
    14521 ggaggggacg ggaacctgat tccgatcaac atcgaagacg agatgcggag gtcttacctc
    14581 gattacgcga tgagcgtgat cgtcgggcgt gccttgccgg acatccggga cggcctcaag
    14641 cccgttcacc gccgcgttct ttacggtatg cacgagatgg ggctgcacta caaccggcct
    14701 acgaggaagt gcgccaaaat taccggcgag gtgatgggaa aatatcaccc gcacggcgac
    14761 gcctccattt acgacacgct tgtgcgtatg gcgcagccgt tttcgatgcg ttacccgttg
    14821 gtggacggtc aagggaactt cggttccgtc gacggcgacc cgcccgcggc catgcgatac
    14881 accgaggcaa gactgtcgcg catcgcgggc acgctgctcg aggatatcga caaggagacc
    14941 gtcgatttcc ggccgaacta cgacgaaagc gaggtagagc cggaagttct gccaacgcgt
    15001 gtccccaatc tgctcatcaa cggctcgtcc ggcatcgcgg tgggcatggc gaccaacata
    15061 ccgccgcata atctgaccga ggtcgtcaac gccaccatcg cgctgatccg gaatccgaag
    15121 acgccgctgc tcgaaattct gcaaatggtg cccggtccgg acttccccac cggaggcttc
    15181 attctggggc ggcaggggat cttcgacgct ttcaccaaag gccgcggttc cttgaagctg
    15241 cgtgcccggg cagccatcga acatgtcact aaagaccgcg atgcgatcat cgtcaccgag
    15301 cttccatacc aggtgaacaa ggcccggctg gtggccgaca tcgccgcgca ggtgaacgac
    15361 aagaagattg aaggaatttc cgatgtacgc gacgagagcg accgcgatgg catgcgtatc
    15421 gtcgtggaac tgaaacgcgg cgagcaggcc gaagtgattc tgaataacct gtacaaacac
    15481 acgcagatgc aggttaactt cggcgtcatc atgttgtcca tcgtcaatgg gcagccgcgc
    15541 gagctggggc tgatcgactg catcaaacga ttcatcgatc accgcattga tgtggtgcgg
    15601 cggcgcaccg attacctctt gcgaaaagca cgcgaacgcg aacacttgtt gctcggcttc
    15661 aaaaaagcgc tcgaaaacct ggatgccgtg atcgagttga tccgcgcttc cagaaatccg
    15721 agggaagccc gcgaaagcct ggtgacccgg tttgagttca cagaacggca ggcgcaggcg
    15781 atcatcgaac tgcaactcca gcggctcacc gg
//
LOCUS       DASTMK010000219         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_298971, whole genome shotgun sequence.
ACCESSION   DASTMK010000219 DASTMK010000000
VERSION     DASTMK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298971"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..520)
                     /gene="cas2"
                     /locus_tag="VFQ79_09805"
     CDS             complement(230..520)
                     /gene="cas2"
                     /locus_tag="VFQ79_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEU0139995.1"
                     /translation="MLVLIAYDVSTESDGGKRRLRRVAKLCVDYGQRVQKSVFECVVD
                     PAQWARLKHALAQEIDPEKDSLRFYFLGANWRTKVEHVGAHPAYDPEGPLIL"
     gene            complement(527..1588)
                     /gene="cas1c"
                     /locus_tag="VFQ79_09810"
     CDS             complement(527..1588)
                     /gene="cas1c"
                     /locus_tag="VFQ79_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HEU0139996.1"
                     /translation="MKRLLNTLYVTTQGAYLSKEGEAVRVQVGDELKLRLPMHTLTSV
                     VCFGQVSCSPFLLGALAERGIAVAYLTEHGRFLASVHGNTSGNVLLRREQYRAADDGG
                     RSAAIARNVVLAKVANSRNVLLRAARETGRSSDEEAGNHGLPEAAERLASSLRELERE
                     QFLAQGGTVDSVRGIEGDAARVYFGAFGSLLATGDETLRFKGRTRRPPLDPINAVLSF
                     VYTLLVHDVRGACESVGLDPAVGFLHRDRPGRPSLALDLMEELRPHLADRLALSMFNR
                     RQLQRKDFEYQESGAVTLETDARKRVLVAYQERKQEEVLHPFLEERVPIGLIPFVQAK
                     LLARHLRGGLDGYPPYFWK"
     gene            complement(1585..2304)
                     /gene="cas4"
                     /locus_tag="VFQ79_09815"
     CDS             complement(1585..2304)
                     /gene="cas4"
                     /locus_tag="VFQ79_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEU0139997.1"
                     /translation="MGEVTYPESELLPLSALQHLVFCARQCALIHLEQAWEENALTAE
                     GRILHEKTDSAKAEWRGGVRIARSLRIRSLRLGLAGVADVVEFHPVDTDSDNPDDTSE
                     AGVALPGMRGRWRPFPVEYKRGKAKKDDSDRAQLCAQALCLEEMLGVAVPAGALFYGE
                     TRRRLDVAFDARLRGRTVALAEELHTLMASRVTPQAEYEAKCDRCSLIEICMPKLPGS
                     SGRVDRFVAKAIAAAIGERLP"
     gene            complement(2292..3173)
                     /gene="cas7c"
                     /locus_tag="VFQ79_09820"
     CDS             complement(2292..3173)
                     /gene="cas7c"
                     /locus_tag="VFQ79_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEU0139998.1"
                     /translation="MSVPLTNRYDFVYLFDVVDGNPNGDPDAGNLPRVDAETSQGLVT
                     DVCLKRKIRNYVTEAKGGQPPHEIYVKEKAVLNSNNERAYTALGLKPEPKKLPKEEAK
                     AREVTRWMCKNFFDIRAFGAVMTTEINSGQVRGPIQFTIARSIDPVVSAEFSITRCAV
                     TNEKDLEKERTMGRKFTVPYGLYRCYGYINATLAEQTGFTEDDLGLVKDALNNMFELD
                     RSAARGLMAPVRCVAFRHENRLGNARADQLFSRVKVQLRPELKTESRPARTREDYLIE
                     VDRTSLPAGVTVEEWVK"
     gene            complement(3170..>3939)
                     /gene="cas8c"
                     /locus_tag="VFQ79_09825"
     CDS             complement(3170..>3939)
                     /gene="cas8c"
                     /locus_tag="VFQ79_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEU0139999.1"
                     /translation="LGLGPNAARLCVRFWIDSTVGEMKQRLGQHLRDVELIGAGDGGP
                     PLSIRRMVQATGRFKGNGKGYDSDSVSPVLAAAVSRAVLAGTPYPLSLLTAMIGRIRR
                     DGEASHPRVAAIKACLCRLQRMREITKEVSVGLDRDRTEPAYLAGRLFAALERVQTDA
                     LGRDLNSTIKDKYFSSACATPGVVFPRLIRLNTFHLAKLDGGLRVVREKLIGEIVRDL
                     DRFPKFLNLEDQGLFTVGYFHQRQDFFANKRSEGETE"
BASE COUNT          772 a         1361 c         1002 g          804 t
ORIGIN      
        1 cgtttcaatc cacgcccccg cgcggggggc gaccgccagc gtagaacacg cctagctcca
       61 acagcttagc agcgatttcc cgcgaaccca gcgacacatg tacccaagcg gttccacccg
      121 attgcgggtg ttcgcagtaa accgcacaat cgaaaccggt tagccacagc gcgaatctcc
      181 cggcgatttt gccaccgctt ccggtccgcg ctcgcatttc acgccactct cacaggatca
      241 gcggaccctc tggatcgtac gcaggatgcg cccccacatg ctccaccttt gttctccagt
      301 tcgctcccag aaagtagaac cgcaggctat ccttctccgg atcgatctcc tgtgcgagtg
      361 cgtgcttcag ccgcgcccac tgcgccgggt ccacaacgca ttcaaacacc gacttctgaa
      421 ccctctggcc atagtccaca cagagcttcg ccacccgtcg gagtcttcgc ttgcccccgt
      481 cactctccgt ggacacatca taggcgatca gcaccagcat cttcccctac ttccagaagt
      541 acggtggata cccatccagg cccccgcgca gatgtcgtgc gagcagcttc gcctgcacga
      601 atggaatcaa gccaatggga acacgctcct ccagaaacgg atgcagcact tcctcctgct
      661 tgcgttcctg ataagccacc agcacccgct ttctcgcgtc ggtctccagc gtgaccgcgc
      721 cgctctcctg atactcgaag tcttttctct gcagttggcg gcggttaaac atcgagagcg
      781 ccagccggtc tgccagatgg ggccttagtt cctccatcag atccagcgca aggctcgggc
      841 gtccagggcg gtcccggtga agaaaaccca ccgccgggtc caatccaacg ctctcgcacg
      901 ctccccggac gtcatgcacg agcagtgtat acacaaagct caggacagca tttatcgggt
      961 cgagcggcgg ccgccgggtc cgccccttga accgcagcgt ctcatcacca gtcgccagca
     1021 acgaaccaaa tgccccaaaa tatacccggg ccgcgtcccc ttcgataccc ctcacggaat
     1081 ccaccgtgcc gccctgggcc agaaactgct cgcgctccag ttcccgcagc gaactagcca
     1141 gacgctcggc cgcttcgggc aggccgtgat ttcccgcctc ctcgtcgctg ctccgcccgg
     1201 tctcccgcgc cgcgcgcagc agcacattcc ggctgttggc caccttggcc agcacgacgt
     1261 ttcgtgcaat ggccgcggat cgtccgccat cgtcggcggc ccggtattgc tccctccgca
     1321 acagcacgtt cccgctggta ttgccgtgga cgctcgccag aaaacgcccg tgctcggtaa
     1381 ggtaagccac cgcgatgccc cgttcggcca gcgcgcctag cagaaacggg ctgcaactca
     1441 cctgcccgaa acacacgacg ctcgtcagcg tgtgcatcgg cagccgcagt ttcagttcat
     1501 cccccacctg tacccgcacg gcctctccct ctttcgagag ataggcgccc tgcgtggtga
     1561 catagagcgt attcagcagt cgcttcatgg cagccgctct ccaatcgccg cagcaatggc
     1621 ttttgcaaca aagcggtcca cccgcccaga gcttcccggc aacttcggca tgcaaatttc
     1681 gatcagtgag cagcgatcgc acttcgcttc gtattccgcc tggggtgtca ccctcgacgc
     1741 cattagcgtg tggagttctt cggctagcgc cacggttcta ccgcgcaacc tcgcatcgaa
     1801 agcgacgtcc agccgccggc gagtttcccc atagaacagc gcccccgccg gaaccgccac
     1861 gcccagcatt tcctccaaac agagcgcctg cgcacaaagc tgagcccggt ctgaatcgtc
     1921 tttcttcgcc ttccctcgct tgtactccac cggaaacgga cgccaccgcc cgcgcattcc
     1981 cggcagcgcc accccggctt cggaagtgtc atccgggttg tctgaatcgg tatccaccgg
     2041 atgaaattcc acgacgtccg ccaccccggc caaaccgagg cgcaggctgc gaatccgcag
     2101 actccgcgcg atccgcaccc caccccgcca ttccgccttc gccgaatccg ttttttcgtg
     2161 cagaatccgg ccttccgccg tcagcgcatt ctcttcccag gcctgttcca aatggatcag
     2221 cgcgcattgg cgtgcgcaga agaccaaatg ctgcaatgcc gacagcggaa gaagttcgga
     2281 ttcggggtag gttacttcac ccattcctcc accgtgactc ctgcaggcag acttgtcctg
     2341 tcgacctcga tcagataatc ctcccgcgtg cgggcggggc gcgattccgt cttcaattct
     2401 ggtcgtaact ggaccttcac gcgggagaac aactggtcag cgcgggcatt gccgagacga
     2461 ttttcatgcc ggaaggcaac gcaacgcacc ggggccatca atcctcgcgc tgccgagcgg
     2521 tcgagttcga acatgttgtt cagggcatcc ttgacaaggc ccaaatcgtc ttcggtgaat
     2581 cccgtctgtt ccgccaacgt ggcattgatg tacccgtagc accgatagag cccgtacggc
     2641 accgtgaact tgcggcccat cgtccgttcc ttctccaaat ccttctcatt ggtcaccgcg
     2701 cagcgggtga tggagaactc cgccgaaacc acgggatcga tgctccgggc gatcgtaaat
     2761 tgaatcgggc cacgaacctg gccagaattg atctccgtcg tcatcactgc accgaacgct
     2821 cggatgtcaa agaagttttt gcacatccat cgcgtcacct cgcgggcctt cgcctcctct
     2881 ttgggcagtt tctttggttc cggctttagc ccgagtgctg tatacgccct ttcgttattc
     2941 gaattgagta cagccttttc ctttacgtag atctcatgag gaggttggcc acccttggct
     3001 tcagttacgt agtttcgaat cttccgcttt aagcagacgt ccgtgacaag cccctggctt
     3061 gtctcggcat ccacacgcgg cagattaccc gcatcaggat ctccgttcgg attgccgtca
     3121 accacatcaa agaggtaaac gaagtcgtat cgattcgtta ggggcacgct cattctgtct
     3181 ctccttccga tctcttattc gcaaaaaagt cctgtcgctg gtgaaaatat ccgacggtaa
     3241 acaacccctg atcctcgagg ttcaggaact tcgggaaccg atccaaatcc ctgacgatct
     3301 ctccgatcag cttctctctg acgacacgca gccctccatc cagctttgcc aagtgaaagg
     3361 tattcaggcg gatgaggcga ggaaacacaa ctcctggcgt cgcgcacgca gaagagaaat
     3421 acttgtcctt gatcgttgag ttcagatcgc gtccaagcgc gtccgtctgc accctttcca
     3481 acgcggcaaa gagtcgaccc gcaagatatg ccggctccgt tcggtctcga tctaatccca
     3541 cactgacctc cttggttatt tcgcgcattc tctgcaagcg gcataggcac gccttgatag
     3601 cagcgactct cggatgagaa gcctctccgt ctcgccggat tcggccgatc atggctgtga
     3661 gaagcgagag gggatatggc gtcccggcga gtacagcgcg cgaaacggcc gctgcgagca
     3721 ccggagaaac gctatcgctg tcgtacccct tcccattgcc tttgaatcgg ccggtggctt
     3781 gtaccatcct gcggatgctg agcggagggc ctccgtcacc agccccgatc aattccacgt
     3841 cccggagatg ctgtccgagc cgttgcttca tttcgcccac agtgctgtca atccagaagc
     3901 gcacgcatag tcgcgcagca ttcggaccca atccaagga
//
LOCUS       DASTMK010000220        14149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_266630, whole genome shotgun sequence.
ACCESSION   DASTMK010000220 DASTMK010000000
VERSION     DASTMK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266630"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFQ79_09830"
     CDS             <1..1038
                     /locus_tag="VFQ79_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140000.1"
                     /translation="FEGVEPGVYEATTEGARAISVNLTHAWCSYVVLLMKPGVIAYTR
                     APEGAPPWPVQNICTVRSDGSDDRCLTTDDHSHNPSWSPDGTRILFIHDSTLSTKPPY
                     RETEDTRSHHPIELSVMDADGGNRRVLRRIEPVVHSAAWSPDGETLAITAATAPTAGE
                     QPPVGLFLLPATGKGELRLFRSNAWTPSWSPDGRKLAFTVEHPRGHWRVHTANVDGTG
                     ETALGSPDADNGSPAWSPDGREIAFDQFTDANRRQQVFVMKPDGADVRQVTSDPAWSC
                     ARPSWSASGTHLAVSCRSAGSFCGMGFFSTGQKMPECTRRVFLVPAATESAASAIRLF
                     DHDGAMASFAL"
     gene            1453..3174
                     /locus_tag="VFQ79_09835"
     CDS             1453..3174
                     /locus_tag="VFQ79_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HEU0140001.1"
                     /translation="MAKTLIAHEQPIAKIFSDDYVFRIPGYQRPYAWTTDQAQELLDD
                     LLTFMQAAGRPVEEMPPYFLGSIVLIKEEAAPQADVVDGQQRLTTLTILLAAIRASVS
                     EPSASDLTKLIYEKGSAILGTKDRFRLTLRERDSDFFQSYIQKEDGIDKLLKVNGDLK
                     DSQHNIRANATLYTNSLATLPEADRLRLAQFVVTRCYLVVVATPDLDSAYRIFSVLNS
                     RGLDLAPTDILKSEITGGIAAGQREVYTKKWEDAEEDLGREAFTDLFSHIRMVYRRLK
                     PKGTLIKEFKDHVTEAKQPAQFIDHVLIPMAKAYEEVLDAGYESTAHAETVNEYLKWL
                     NRLEFNDWVPPALAFTTRHHQDPAAMVAFFRGLERLAYAMLIQKSGINGRIERFSQLT
                     SAIINGSSLYDEKSPLQLTPNEQYLVYTQLDGMVYDTFAARARTTILLRLDILVSGGG
                     AKYDYQTITVEHVLPQNPPADSKWLTWFPDVKDRYQWVHKLGNLALLTRKKNSAASNY
                     EFDKKKQSYFAQGGVSPFVLTTQVLNKNEWTPAIVAARQKELLGILEAHWQLKNRKNP
                     AMAATQP"
     gene            complement(3459..3941)
                     /locus_tag="VFQ79_09840"
     CDS             complement(3459..3941)
                     /locus_tag="VFQ79_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0140002.1"
                     /translation="MELRFQVQTRINKPLNEVFDAVYNPDKLRAYFANGGASGPLNEG
                     STVIWRFAEFPADVPVTVKRVVPGERIVLEWEASEYDDFKPEEGELPPSAGYNTTVEI
                     AFEALSPESTMIRISESGWRETQKGLNSSYGNCQGWTHMMCCLKAWIEHGINLRDGSI
                     "
     gene            3985..4314
                     /locus_tag="VFQ79_09845"
     CDS             3985..4314
                     /locus_tag="VFQ79_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0140003.1"
                     /translation="MSSEKDRQDAVFKALADGRRREMLDLLRDGPKTTGELCRHFDSL
                     DRCTVMQHLRVLEAADLILVKRQGRQRWNYIHPIPIKEIYDRWISRYAVGAVDLLARM
                     KRQMEEE"
     gene            4380..7001
                     /gene="clpB"
                     /locus_tag="VFQ79_09850"
     CDS             4380..7001
                     /gene="clpB"
                     /locus_tag="VFQ79_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEU0140004.1"
                     /translation="MDPNRFTEKVQEAIRSAQSIAVRYGNQQVDAEHLLLALLDQEGG
                     LATSIFNKADVNGPALRRRLEDEINRLPKVSGPGGASDQVYITGRLQRILTQAEDEAK
                     RLKDEYISVEHLLLALVDDSGAAGRILKEFNVTRDRLMRALQEVRGSQRVTSANPEAT
                     YEALERYGRDLTQLAAQGKLDPVIGRDDEIRRVMQVLSRRTKNNPVLIGEPGVGKTAI
                     AEGLAQRIVRGDVPEGLKNRRVVSLDMGALIAGAKYRGEFEERLKAVLKEVQSAEGEI
                     ILFIDELHTVVGAGKAEGAMDAGNLLKPMLARGELHCIGATTLDEYRKNIEKDAALER
                     RFQPVLVDQPSVEDTISILRGLRERYEVHHGVRIKDTALVAAAVLSNRYISDRFLPDK
                     AIDLVDEAAAKLRTEIDSMPAELDEIMRRIMQLEIEREALKKETDQASKDRLEKLEKE
                     LADLRGEASSLQAQWQAEKEGVQQLRSLREQLEQTRIEIEQAERQYDLNRAAELKYGK
                     VTELQRRLNDEEQRLANKQGQTRLLKEEVDEEDIAEVVSRWTHIPVSKLLEGEMQKLL
                     HLEDELHKRVIGQDEAVLAVSEAVMRARSGLKDPNRPIGSFIFLGPTGVGKTELARAL
                     AGFLFDDERAMIRIDMSEYQEKHTVARLLGAPPGYVGFEEGGQLTEAVRRRPFAVILF
                     DEIEKAHHDVFNVMLQILDDGRLTDGQGRTVDFKNTIVVMTSNVGSHRILEYRGAFEG
                     HEYERMKDAVLEELRRGFRPEFLNRVDEIIVFHALSEEHLKKIVDIQLSGLRRRLEDR
                     KIRIELTDAAREHLVRAGYDPNYGARPLKRAIQREIETPLARRMLQGEVRDGSSVVVD
                     ADGSGELQFTIEPATVT"
     gene            7080..8525
                     /locus_tag="VFQ79_09855"
     CDS             7080..8525
                     /locus_tag="VFQ79_09855"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU0140005.1"
                     /translation="MKSMMFNSVSCVLATAVLMAVPAYAQKGAIQDSYAAVVKKALPA
                     VVNISTSRIVRPPRGGELPQLPDFFDFFGFGGPGGRDFGPAPAPAPRERRTQSLGSGV
                     VVSADGYILTNNHVVEEATDITVAFDDRREFSAKLIGGDALSDIALLKVEGTGFATLP
                     MGNSDSIEVGDICLAIGNPFGLKSTVTMGIISATGRGGLGIETYEDFIQTDAAINPGN
                     SGGAMIDTRGELIGINTAILSRTGGNQGIGFAIPINMAKDIMGQIRDTGKVSRGYMGA
                     GIQDLTPELAKAFNLSSANGVAITQVEQDSPAEQAGLQPGDVVVSVNGNVVKTANELR
                     LRISGTKPGETVKLGVQRDGKPVALAVTLKQLPSQRAEEESGGGGGGEPSALEGLSVD
                     DLTPQIAQQLGLSPEVKGVVVTSVDPSSDAANSGLARGDVIQSVNRQPVTSVAEFRKL
                     FGSTAGQPVVLLVNRRGATRFVVVRPQGGNR"
     gene            8529..8894
                     /locus_tag="VFQ79_09860"
     CDS             8529..8894
                     /locus_tag="VFQ79_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU0140006.1"
                     /translation="MSNYMRQSVFFPQRYAEGVWRPAADIYRTQTGWILKFDLAGVRP
                     EDILVQILGCRVSVSGVRRDWVLEEGCTYYCMEISYSSFERTVDLPCELHGTQWTVEF
                     LHGFVLVRLDLTGENRERK"
     gene            8881..11238
                     /gene="lon"
                     /locus_tag="VFQ79_09865"
     CDS             8881..11238
                     /gene="lon"
                     /locus_tag="VFQ79_09865"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU0140007.1"
                     /translation="MNENNENVQILPVLPLKNTVLFPFLLMPLSVGRPHSIGAVEAAL
                     ATEGKEIIVVSQKDPSVDTPGQDELYTIGTKAVVRKMSRPNEESMELLVLGGERVVLI
                     KLEQTEPYMTGRVRPYPLPEDGGPEVEALHQSIVELAGKAISLAQPQATPEITRLLAG
                     ADDPLRVVYMLASMFSLDLDKEQALLEAQTRLDALRLMHSYLNHEVQVLELRTQIASS
                     ARSEMSKEQREYLLRQQMRAIQQELGEKNPEQADIDLLRERFEKTELPEDIRKEVERE
                     LSRLERLPGASPEYHVSRTYIDLILEMPWKASTEDHLDIARAREILDEDHFNLKEVKE
                     RILEHLGVLKLNPGAKAPILCFVGPPGTGKTSLGQSVARALGRKFERMSLGGMHDESE
                     LRGHRRTYIGAMPGRLIQAVRRAGSNNPVILLDEVDKLGRDFRGDPAAALLEILDPEQ
                     NKNFRDNYLDLPFDLSKVFFITTANTLDTIPRPLLDRMEILRLSGYSEEEKIEIARRY
                     LIPRQLKEAGLTEAQLNFTDEGLRAIISGYTREAGLRRLEQAIGRVARKAALRVAEGN
                     EAALTVRPEDLYDMLGPQMFTPEQARKELAPGVATGLAWTESGGDVLYLEATLLPDGK
                     GLTLTGQLGEVMQESARAAQSYVWSHAEELGIDHALFKDAGVHLHVPAGAIPKDGPSA
                     GVTAVVALASMYSKCPVRKDTAMTGEITLSGLVLPIGGVKEKALAARRAGIKRIVLPK
                     ANEKDLRELPENVRNEIEFIPAERIEDVLLNTIPALTDRLAFTTR"
     gene            11282..12430
                     /locus_tag="VFQ79_09870"
     CDS             11282..12430
                     /locus_tag="VFQ79_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU0140008.1"
                     /translation="MRSLFLLGSLFLPLAAEMRFQAQEIATGFGVTYAVTIADVNRDG
                     KPDIVAINPTQVVWFENPAWTKHVILDGATKKDNVCIAPYDIDGDGYVDFAVGADWQP
                     VNTESGGSLQWIGRPAAHRSGPWSLTPITEEPTLHRIRWGDVDGDGRAELIVAPLHGR
                     GNQRPDWNGQGARILVFKIPKNPASEPWPMEVADNSLHIVHNFLVTDFNRDGRDDLIT
                     ASREGVHVLAREKDGKWRKTRIGEGSPGEIKLGRSGGKRVLATVEPWHGNSVVIYSEP
                     ENSGAQWPRVVVEDQLHQAHALGWGDFDGDGDDELAVGWREGAFGVAVYKRGGEGRFE
                     KIMVDEGGMAAEDLVVADLDSDGRPEIVAGGRKTANIRIYWNVAGRHK"
     gene            complement(12421..>14149)
                     /locus_tag="VFQ79_09875"
     CDS             complement(12421..>14149)
                     /locus_tag="VFQ79_09875"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEU0140009.1"
                     /translation="EVVTGLAFFIGFVTLVIAPAPGFSLPASVPLAAALLFVARRFEW
                     TRMAVAGLVFTYSVYALEAQPEWAMVGHPVLWLYWILFEGFDLLRITTDRRGESIGRT
                     LFPLNACGFLGAALLRAREIDYFLFAAAAAYAVTAIVRAKLRRREAAVAPDQGLFAMV
                     PGTFEAAVTISAGVLAAAFLQRFTGWRANIALLMEAEFLVFSGVLLRERYLRTLGALV
                     FAVPVLKLLLVDVPQGGEFQTFGPALFNWTPAAFIIMAAAFVNRVLTRSGPAYTHAGV
                     LLLALVLAFELPHAQIGLWWMIAAIPMFAAAERTFVTELRYDAYALGTLGTIFLWLTQ
                     LSRTHPWWPVAAGAALACAATIAAFINRPRSEPERQLVDLFTSGAAVLLSATLFWRVL
                     DERAIAPVWAAICAGLVLAARRINVPKLQWHAYVLAVATFFRAWTVNLEEANWRILST
                     AAAIGGLYAAQFLSPHRTYGRAAFSALATLLTTLLLISEVSGRLLTVAFGLEGVVLLS
                     AGFAGRDRVLRLSGLGLFFVCVTKLFVYDLRELDTLNRIISFIVLGLLLLAASWIYTR
                     FREQIKRYL"
BASE COUNT         3152 a         4141 c         4331 g         2525 t
ORIGIN      
        1 tttgaaggcg tggaacctgg agtttacgaa gcgacaacgg agggcgcacg ggcgatcagc
       61 gttaacctca ctcacgcctg gtgttcgtac gtcgttttgc tcatgaagcc gggagtcatc
      121 gcctacacgc gtgcccccga aggcgctccg ccgtggccgg ttcagaatat ctgcaccgtg
      181 cggtccgatg gctccgatga tcgctgtctc acaacggacg atcatagcca caatccatca
      241 tggtcgcccg atgggacgcg catcctcttc attcatgact ccacgttgag cacgaagccg
      301 ccgtatcggg aaacagagga caccagatcg caccatccaa tcgagttgtc cgtaatggat
      361 gcggatgggg ggaatcgccg ggtcctccgg cgcatcgagc cggttgtaca cagcgcagcg
      421 tggtcacccg acggtgagac tcttgcgatc acagccgcaa ccgcaccaac ggctggtgag
      481 cagccgccgg tcggcctgtt tcttctaccg gcaacgggca aaggcgaact tcggctgttc
      541 cgatcaaacg cctggacgcc atcgtggtct ccggacggcc ggaagttggc cttcactgtc
      601 gagcatccac gcggacattg gcgagtacac acggctaatg tggacggaac gggtgaaacc
      661 gccctcggca gccctgacgc cgacaacgga tcaccggcat ggtctccgga tggtcgcgag
      721 attgctttcg accagttcac agacgcgaac aggcgccagc aggtattcgt gatgaagccc
      781 gacggcgcag acgtccggca agtcacctct gatccagcct ggtcttgtgc ccggccgtcg
      841 tggtcagcga gcggtactca ccttgcagtc tcctgccggt ccgccgggtc gttttgcggg
      901 atgggttttt tctctacagg ccaaaagatg ccggagtgta ctcgccgcgt gttccttgtg
      961 ccggctgcaa ccgaatccgc agcctcagca atcaggctgt tcgaccacga cggagcgatg
     1021 gcgtcgtttg cactgtgatg atgcgtcctt ccagcgcgat gtggaatcac ctgccggaat
     1081 ccggactcgc cgtccagcgc ttaacgctaa agacggctcg tcgctaccgg agcgttcccc
     1141 gtctcatcgt ttcggatgcg agccagtctc ccagttccag gtcgcgtgat ccagaaacaa
     1201 acccgacgat ccacatctcc gacatgactc ttcggccaat tgcttccgag tccggatgat
     1261 tcacgcagga tggctacggc gtattacgag agattccgat ctcaacctgc tatagttctt
     1321 ggtgcgaagc gaatcgagtc gaagaagtcg cggttggcgc cggctaattc cgtgcggccc
     1381 cgaaaacggg ttccaccggc gtcgcggtat tttcgaacca gtttcgcgac acatcttccg
     1441 aaagaacgac caatggcgaa aacactgata gcgcacgagc agccgatagc gaagatcttc
     1501 agcgacgatt acgtctttcg cattccgggc taccaacggc catacgcatg gacgacggac
     1561 caagcgcagg agctcctcga tgatctgctc actttcatgc aagccgccgg gcgaccagtg
     1621 gaagagatgc cgccgtactt tctcggcagt atcgtgctca tcaaggagga agctgcgccc
     1681 caagccgacg tcgtcgatgg acaacagcga ctcactacgc tgaccatcct gctggcggcg
     1741 atccgcgcga gcgtgagcga acccagcgca tcggatttaa ccaagctcat ctacgaaaag
     1801 gggagtgcca tactcggcac gaaagaccgg ttccgcctca cgcttcgcga gcgggacagc
     1861 gacttcttcc agagctacat ccaaaaggaa gatggcatcg acaaactcct caaagttaat
     1921 ggtgacctga aggacagtca acacaacatc cgcgcgaacg caacgctata caccaatagc
     1981 ctcgcgactc tccccgaagc cgaccgactg cgactcgcac aattcgtcgt tacgcggtgc
     2041 tacctcgtcg tagttgcaac tcccgatctc gactccgctt atcgcatctt ctctgtcctc
     2101 aacagccgcg ggctcgatct cgccccgacc gacatcctga aatcagaaat taccggtggt
     2161 atagcagcgg gacaacggga ggtgtacacg aagaaatggg aggacgccga agaagacctc
     2221 ggccgagaag ccttcacgga tcttttcagc catatacgga tggtgtaccg acgcctcaaa
     2281 ccgaaaggca cgctcatcaa ggagttcaaa gaccacgtca ctgaggcgaa gcagccagct
     2341 cagtttatag accatgttct tatccccatg gcgaaagcgt atgaggaagt tctcgacgcg
     2401 ggttacgaga gcacggccca cgccgagacg gtgaatgaat acctcaaatg gctaaaccgc
     2461 ctcgaattca acgactgggt accgccggcc ctcgctttca ctacacggca tcatcaagat
     2521 cccgcggcga tggttgcctt ttttcgcggt ctcgaacgac tcgcatatgc gatgctcatc
     2581 cagaagtccg ggatcaacgg tcgcatcgaa cgattctcgc aactcacgag cgcgatcatt
     2641 aacggcagca gcctctacga tgagaagtca ccgctccaac tcacgccgaa cgaacaatac
     2701 ctcgtctaca cacagcttga tgggatggtt tacgacacat tcgcagcgcg agcacgcacg
     2761 accatcctcc tccgactcga tatcttggtc agtggcggcg gcgcaaagta cgactaccag
     2821 accatcacgg tagagcacgt actgccacag aatccgccgg cagacagcaa gtggctcaca
     2881 tggttccccg atgtgaaaga ccggtaccaa tgggtccaca aactcggcaa tctcgcgctg
     2941 ctcacgcgca agaagaacag tgccgcctca aattacgagt tcgacaagaa gaagcagtct
     3001 tatttcgccc aaggtggcgt ttcccccttc gtcctaacga cccaagttct gaataagaac
     3061 gaatggacgc cagcaatcgt agccgcgcga caaaaggaac tcctcggtat cctcgaagca
     3121 cactggcaac tcaagaaccg caaaaatccc gcgatggccg cgacgcaacc atgacaacgc
     3181 ttagaggttg aggggcattc ggaggttctg ggaacctccc gtagcaacgg ctacacaaag
     3241 caggacgtta gtttgccccg gcgtgtggat cgggcctaga gccgccatat ccccgtcgga
     3301 tcacgccctt tccgcaacca gggcccttca gcgtcgcgtt gacgaaaaac gcctttaccg
     3361 gagtgtggcg aaccctctcc atcgcaaacg cctaggcgct atccggaagc ctacggtccc
     3421 gtcactgtgg cgcagcgttg cagccggccc gagcgcgttt agatcgatcc gtctctcagg
     3481 ttgatgccgt gctcgatcca tgccttcagg cagcacatca tgtgcgtcca tccctggcag
     3541 tttccataag agctgttgag gcccttttgc gtctcccgcc aacccgattc ggagattcgg
     3601 atcatcgtcg attcggggct cagggcttcg aacgcgatct ccactgtcgt gttgtaccca
     3661 gcgctcggcg gcagttcccc ttcttcgggt ttgaagtcgt catactcgga ggcttcccat
     3721 tcgagtacga tccgttcgcc tggaacgacc ctcttgaccg tcaccggcac gtccgccgga
     3781 aactcggcga acctccagat caccgttgag ccttcattga gcggcccgct ggcgccaccg
     3841 ttcgcaaaat aagcgcgcaa tttgtccggg ttatacacgg catcgaagac ctcgttgagc
     3901 ggcttgttga ttctcgtctg tacctgaaat cgcagttcca cggtttctcc tccagttcag
     3961 aattttatgt tatatttata taacatgtca agcgagaagg accggcagga cgcagtattc
     4021 aaggcgcttg ccgatggccg caggcgggag atgctcgatc tgctgcggga tggcccgaag
     4081 accaccggag agttgtgcag gcatttcgac agcctggacc ggtgcacggt gatgcagcat
     4141 ctccgtgtgc tggaagcagc ggacctgatc ctcgttaagc ggcaggggcg gcagcgatgg
     4201 aactatatcc acccgatccc gatcaaagag atctacgacc ggtggatcag ccggtacgcg
     4261 gtgggtgcag tggatctgct ggcgcgcatg aagcggcaga tggaagaaga atgactcggt
     4321 tgacagtgtt aacctatcag cgatatgctg atataagttg atagatagat actaaactta
     4381 tggaccccaa tcgcttcacc gaaaaggtac aggaggccat ccgcagcgcc caatccatag
     4441 ccgttcgata tggcaaccaa caggtcgacg ccgagcacct gttgctcgcg ctcctggacc
     4501 aggaaggcgg ccttgctact tctatcttca acaaagcaga tgtcaatggg ccagccttac
     4561 gccgccgtct agaagacgaa atcaaccgtt taccgaaggt ctcggggccg ggtggagcgt
     4621 ccgaccaggt atacataacg ggtcggcttc agcgcatcct tacgcaagcc gaagacgaag
     4681 ccaagaggct gaaggacgaa tacatctccg ttgaacacct cctgctcgcc ctggtggacg
     4741 attcgggtgc agccggacga attctgaaag aattcaacgt cacgcgcgac cgcctcatgc
     4801 gggcacttca ggaggtacgc ggcagccagc gcgtaacctc ggccaacccg gaggcaacct
     4861 atgaggcgct ggagcgctac gggcgcgacc tcacccaact ggcggcacag ggtaaactcg
     4921 atccggtgat tggccgggac gacgagatcc gccgggtcat gcaggtgctt tcccggcgca
     4981 cgaaaaacaa cccggttctc attggcgaac caggtgtcgg aaaaacggcc attgccgaag
     5041 gcctcgcgca gcgcatcgtg cgcggcgacg ttcccgaggg cctgaaaaat cgccgcgtgg
     5101 tttcgctgga catgggcgcg ctgatcgccg gcgccaagta ccgcggcgaa ttcgaggaac
     5161 gtctgaaagc cgttctgaaa gaggtgcagt cggcggaagg cgaaattatc ctgtttatcg
     5221 acgagcttca taccgtggtc ggcgccggaa aggccgaggg tgcgatggat gccgggaacc
     5281 tgctgaagcc gatgctcgcg cgcggagaac tgcactgcat cggcgccact acgctcgacg
     5341 agtaccgcaa gaacatcgaa aaagacgccg ccctggaacg gcgtttccag ccggtgctgg
     5401 tggatcagcc atcggtcgaa gacaccattt ccatcctccg cggattgcgc gagcgatatg
     5461 aagtgcatca cggcgtgcgc atcaaggaca ctgccctggt agcggccgcg gtgctctcga
     5521 accggtatat ctcggatcgc tttctgccgg acaaggccat agacctggtg gacgaggcgg
     5581 ccgccaagct gcgcaccgaa atcgattcca tgcccgcgga gttggacgaa atcatgcggc
     5641 gcatcatgca gctcgaaatc gagcgcgagg cgttgaaaaa ggaaaccgac caggcgtcga
     5701 aggacaggct cgaaaagctc gaaaaagagc tggcggattt gcgcggcgaa gcgagttcgc
     5761 tccaggccca gtggcaggcg gagaaggaag gggtgcagca gcttcgttcg cttcgcgagc
     5821 agttggagca gacccgaatt gaaatcgagc aggccgagag gcagtacgac ctgaaccgcg
     5881 cggcggaact gaagtacggg aaggtgacag aactgcaacg gcggctgaac gacgaggagc
     5941 agcgactggc gaacaagcag ggccagacgc ggctgctcaa ggaagaagta gacgaagaag
     6001 acatcgccga agtcgtcagc cgttggacac atatccccgt ttcaaaactc ctcgaagggg
     6061 agatgcagaa gcttcttcat ctggaggatg agctgcacaa acgggtcatc ggccaggatg
     6121 aggcggtact ggcggtttcc gaagccgtca tgcgcgcgcg ttccggcctg aaggacccga
     6181 accggcccat cgggagcttc attttcctgg gacccacggg tgtcggtaag acggaactgg
     6241 cgcgtgccct ggccggcttt ctgttcgatg atgagcgggc catgatccgc atcgacatgt
     6301 ccgaatacca ggaaaaacat actgtggcac gcctgttggg cgcgcctccg ggctacgtcg
     6361 gttttgaaga aggcggccag ttgacggaag cggtccggcg ccggcccttt gccgttattc
     6421 tctttgacga gatcgaaaaa gctcatcacg atgtgttcaa cgtgatgctg caaattctgg
     6481 acgacggacg gttgacggat gggcagggcc gcacggtcga tttcaagaat acgatcgtcg
     6541 tcatgacctc caatgtcggc agccaccgca ttctcgaata ccgcggcgcc ttcgaagggc
     6601 acgagtacga gcgtatgaaa gacgccgtgc tcgaagagct ccggcgcggc tttcgtccgg
     6661 agtttctgaa ccgtgtggat gagatcatcg tgttccatgc cctctctgag gagcacttga
     6721 agaagatcgt cgacatccag ctctccggtt tgcgccggcg cctggaagac cgcaagatcc
     6781 gtattgagct gaccgatgcc gcccgcgagc acctggttcg cgccggctac gaccccaatt
     6841 acggagcgcg gccattgaag cgcgccattc agcgcgagat cgagacgccg ctggcgcggc
     6901 gaatgctgca aggcgaagtg cgggacggca gctcggttgt cgtggatgcg gacggctctg
     6961 gagaactcca gttcacgatc gagccggcaa ccgttacctg acagctaatc cggtggggtt
     7021 ctttgatacc cacctgcaat tgcacgcgtc taaacagagg aaggaaaaga gaaaagttca
     7081 tgaaatccat gatgttcaat tctgtttcat gcgttctcgc gacggctgtg ttgatggccg
     7141 tgcccgctta tgcgcagaaa ggtgcaatcc aggacagtta cgctgcggta gtcaaaaaag
     7201 ctcttccggc ggtggtcaat atatcgacgt cgcgtatcgt acgcccgccg agaggcggtg
     7261 aactgccgca actgcccgac tttttcgatt tcttcggctt cggaggcccc ggcggccgcg
     7321 atttcgggcc ggccccggcg cccgcgccgc gcgaacggcg cacgcagagc ctgggttcgg
     7381 gcgtcgtggt atccgccgac ggatacatcc tgacgaataa tcacgtggtg gaggaagcga
     7441 cggatatcac cgttgccttt gacgaccggc gggagttctc agccaaactg attggcggcg
     7501 atgcgctgag cgacattgcc ctgctgaaag tggagggcac cggcttcgcc accctgccga
     7561 tggggaattc ggattcgatc gaggtggggg atatctgcct cgccatcggc aatcccttcg
     7621 ggctgaaatc cacggtgacg atgggaatca tcagtgcgac cggacgcggc ggactgggca
     7681 tcgaaacata cgaggatttc atccagaccg atgcggccat caaccccgga aattccggcg
     7741 gggccatgat cgacacgcgg ggagagctga tcggaatcaa cacggcaatc ctgtcgcgta
     7801 ccggcgggaa ccagggcatc ggcttcgcga ttccgatcaa tatggccaag gacatcatgg
     7861 gacagatccg cgacacaggc aaggtgtcgc gcggctatat gggcgccggg attcaagacc
     7921 tgacgccgga gctggccaaa gccttcaacc tgtccagcgc aaacggcgtc gccatcacgc
     7981 aagtcgaaca agacagcccc gccgagcagg cagggttaca gccgggcgac gtggtggtct
     8041 ccgtaaatgg aaacgtggtg aagacggcca atgagctgcg tcttcgcata tccgggacaa
     8101 aacccgggga gaccgtaaag ctcggcgtgc agcgcgacgg taagcctgtc gcgctggcag
     8161 tgacgttgaa gcagcttccg tcgcaacgcg cggaagaaga gagcggcggt ggtggtgggg
     8221 gggagcccag tgctcttgaa ggtctgtcag tggacgatct gacaccccag atcgcacagc
     8281 agcttggtct cagccccgag gtgaagggcg ttgtggtgac aagcgtcgat ccttcgagcg
     8341 acgccgccaa ctcgggcctg gcgcgcggcg acgtcatcca atccgtgaac cgccagccgg
     8401 tgaccagcgt ggcggagttc cggaagttgt tcggctccac cgcgggtcag cctgtcgtgc
     8461 ttctggtgaa ccggcgcggt gctacgcggt ttgtagtggt ccggccacag ggtgggaacc
     8521 gctaacccat gtcgaattat atgcggcagt ctgttttctt tccgcagcgc tacgcggaag
     8581 gtgtgtggcg tccggcggct gatatctacc ggacacagac cggttggatt ctgaaattcg
     8641 atcttgctgg tgtcaggccg gaagacatcc tggtgcagat cctcggatgc cgcgtttcgg
     8701 tgagcggtgt ccggcgggac tgggttctcg aggaaggctg cacttactac tgcatggaga
     8761 tttcgtacag cagcttcgag cgaactgtcg acttgccgtg cgagctccac ggtacgcagt
     8821 ggaccgtgga gttcctgcac gggtttgttc tggttcgttt ggacttgaca ggagaaaacc
     8881 gtgaacgaaa ataacgaaaa cgttcagatc ttgccggtac tgccgctgaa aaatacggtg
     8941 ctgttcccgt tcttgctgat gcccctttca gtgggccggc cgcattccat cggagctgtt
     9001 gaggctgccc tggcaaccga aggtaaagaa atcattgttg tatcgcagaa agacccatcg
     9061 gtggacacac cgggacagga cgagctttac accattggaa ccaaggccgt ggtgcggaag
     9121 atgtcccggc cgaacgagga atcgatggag ttgctggtgc tgggcggcga gcgcgttgtg
     9181 ctcatcaaat tggagcagac cgagccgtac atgacaggac gcgtgcggcc ctacccgctg
     9241 ccggaagatg gagggccgga agtcgaagcg ttgcaccagt ccattgtcga actcgccgga
     9301 aaagcgatct ccctggcaca gccccaggct acaccggaga tcacgcgcct gctggcgggc
     9361 gccgacgatc ctctgcgtgt cgtctacatg ctggcatcca tgttcagcct cgacctggat
     9421 aaagagcagg cgctgctcga agcgcagacc aggctcgacg cgctgcggct gatgcattcc
     9481 tatctcaacc atgaagtgca agtgctcgaa ctgcgcaccc agatcgcgtc gagcgcacgc
     9541 agcgaaatga gcaaggagca gcgggagtac ctgctgcggc agcagatgcg tgccattcag
     9601 caggagcttg gcgagaagaa tccggaacag gccgacatcg atttgctgcg cgagcggttc
     9661 gaaaaaacgg agcttccgga agacatccgt aaagaggtcg agcgggaatt gtcgcggctg
     9721 gagcggcttc ccggcgcctc accggaatac cacgtctcgc gaacgtacat agaccttata
     9781 ctcgagatgc cctggaaggc cagcaccgag gatcatctcg acatcgcccg cgcccgcgag
     9841 atcctggacg aagaccactt caaccttaaa gaggtcaagg aacgcattct cgaacacctg
     9901 ggcgttctga agctcaatcc cggtgcgaaa gcaccgatcc tgtgcttcgt tggacctccc
     9961 ggaaccggaa agacatcgct cggtcaatcg gtggcgcgcg ctctgggccg caagttcgag
    10021 cggatgagcc tgggcggcat gcacgatgaa tccgagcttc gcggccaccg gcgtacgtat
    10081 atcggagcca tgcccggaag actgatccag gcggtgcggc gcgcgggttc gaacaacccg
    10141 gtgattctgc ttgacgaggt cgacaaactc ggccgcgatt tccgggggga cccggcggcg
    10201 gcgctgctgg agatcctcga ccccgagcag aacaagaact ttcgggataa ctatctcgat
    10261 ctgccgttcg acctgtcgaa ggtgttcttc atcaccacgg cgaatacgct ggatacgatt
    10321 ccccgcccgc tgctcgaccg gatggagatt ctccggctgt cgggttatag cgaagaggaa
    10381 aaaatcgaaa tcgcacggcg gtatctgatt ccgaggcagc tcaaagaagc gggcctcacg
    10441 gaagcgcaac tcaacttcac ggatgaagga ttgcgggcca ttatctccgg ctatacgcgg
    10501 gaagcgggtt tgaggcgcct ggagcaggcg atcggacgtg tagcgcgcaa ggccgcgttg
    10561 cgggtcgccg aaggcaacga ggctgcgctc accgtgcggc cggaggatct ctacgacatg
    10621 ctgggcccac agatgttcac tccggaacaa gcccggaaag aactggcccc gggtgtcgct
    10681 acgggcctcg cctggacgga aagcggtggt gacgtgctgt atctcgaggc tactctgttg
    10741 cccgatggca aggggctgac gctaaccggg cagcttggtg aagtcatgca ggaatccgcg
    10801 cgggcggcac agagctatgt ctggtcgcat gccgaggagc ttggcatcga ccacgcgttg
    10861 ttcaaagacg cgggcgtgca tctgcatgtg cccgccgggg ccattcccaa ggacggtccg
    10921 tcggccggcg tgacggcggt ggtggcgctt gcctcgatgt acagcaaatg cccggtgcgc
    10981 aaggatacgg caatgaccgg cgaaatcacg ctttccggcc tggtgctgcc gatcggcggc
    11041 gtgaaggaga aagcactggc ggcccggcga gccggcatca agcgtatcgt tctgccgaaa
    11101 gccaatgaaa aggatctgcg cgagctgccg gaaaacgttc gcaacgaaat cgaattcatt
    11161 ccggccgaga gaatcgaaga cgtacttttg aacacgattc cagctttaac ggatcgtctc
    11221 gcgttcacca cacgataatt ccaggccgcc gggcggtcta cagatagggc aaaatcgggc
    11281 tatgcggagt ctgtttctgc tgggatctct tttcctgccg ctggcggccg agatgcggtt
    11341 tcaagcccag gaaatcgcca ccgggttcgg tgtgacgtac gccgtgacca tagcggatgt
    11401 aaatcgcgac ggaaagcccg atatcgtcgc gatcaatccc acccaggttg tctggttcga
    11461 aaacccggcc tggacaaagc acgtcatcct ggatggcgct acgaagaaag acaacgtatg
    11521 cattgcgcct tatgacattg acggcgatgg atacgtcgat tttgccgtgg gcgccgactg
    11581 gcaacccgtg aataccgagt cgggcggaag cttgcagtgg atcgggcgcc cagccgcgca
    11641 tcggagcggg ccgtggagtc tgacaccgat cacggaagaa cccacattgc accgtatccg
    11701 ttggggcgat gtggacggcg acggaagggc ggagctcatc gtcgcgcctc tgcacggacg
    11761 cggcaaccaa aggcctgact ggaacgggca gggcgcccga atactggtgt tcaaaattcc
    11821 gaagaacccc gcgagcgagc cgtggcccat ggaggtggca gacaactcgc tgcacatcgt
    11881 ccataatttt cttgtcaccg atttcaatcg agacggccgc gacgatttga ttaccgccag
    11941 ccgcgaaggc gtgcacgtcc tggcgcggga gaaagacggg aagtggcgga agacgcgaat
    12001 cggcgaaggg tctcccggtg aaatcaagtt gggccgctca ggcggcaagc gtgtgctggc
    12061 gacggtggag ccctggcacg gaaactccgt tgtgatctat tccgaaccgg agaatagcgg
    12121 cgcccagtgg ccgcgcgttg ttgttgaaga ccaactccac caggcacacg cactcggatg
    12181 gggagatttc gacggcgacg gcgacgacga actggctgtg ggctggcgcg aaggcgcttt
    12241 tggcgtagcg gtgtacaaac gcggcggcga aggacgtttc gaaaagatca tggtcgacga
    12301 aggcggaatg gcggcggagg acctggtggt cgcggacctc gacagcgatg gccggccgga
    12361 gattgttgcc ggcggcagaa aaacggcaaa catccggatt tattggaacg tggcgggccg
    12421 tcacaaatag cgcttgatct gctcgcggaa ccgtgtgtaa atccaggagg cggcgagcag
    12481 cagcagaccc agcacaataa acgaaataat gcggttgagc gtatcgagtt cgcgcagatc
    12541 gtacacaaag agcttggtta cgcatacaaa gaacaaaccc aggcccgaaa gccgcaggac
    12601 tctgtcgcgg ccggcaaagc ccgccgaaag cagaaccact ccttcgaggc cgaacgccac
    12661 cgtgagaagg cgcccggaca cctcgcttat cagtagcaag gttgtcaata gcgtggccag
    12721 agcggagaac gcagcgcgcc cgtacgtcct gtgcggagac aggaactggg ccgcatacaa
    12781 gccaccgatt gcggcggcgg tggagaggat acgccagttc gcctcttcca ggtttaccgt
    12841 ccaggcgcga aagaacgtgg cgacggcaag cacataggcg tgccactgca gcttgggcac
    12901 gtttatgcgc ctcgccgcga ggaccaggcc cgcgcaaatc gccgcccaaa ccggcgcaat
    12961 ggcgcgctca tccaacacgc gccagaagag cgtggccgat agcagaacag cagcgccgga
    13021 agtgaagagg tccacaagct gccgttccgg ttccgagcgc ggccggttga taaaagccgc
    13081 gatggttgcg gcgcacgcga gagcggcgcc ggccgcgacg ggccaccagg ggtgagtccg
    13141 ggaaagttgt gtcagccaca ggaagatggt tccgagcgta ccgagcgcat aggcgtcgta
    13201 acggagttcg gttacgaacg ttctttcggc ggcggcgaac atgggaatgg cggcaatcat
    13261 ccaccacagg ccaatctgtg cgtgcggcag ctcaaaggcc agcaccagcg caagcaatag
    13321 aactccggcg tgagtgtatg ccggtccgga ccgcgtgagg acacggttca cgaacgcggc
    13381 agccataatg atgaaagccg ccggggtcca gttgaaaagc gcgggaccga aagtctgaaa
    13441 ttcgccgccc tggggaacat ccaccagcaa cagctttagc accggcactg cgaacaccag
    13501 cgcgcccagt gtccgcaagt agcgctcgcg aagcaggacg cctgaaaaca ccagaaactc
    13561 cgcttccatc agcaatgcga tgttcgcgcg ccaaccggtg aaacgctgta aaaacgcggc
    13621 agccagaact ccggcggaga tggttacagc cgcttcgaac gttcccggga ccattgcgaa
    13681 aagaccctgg tccggagcaa cggccgcctc gcggcggcgc aacttcgcac gcacgatggc
    13741 ggttacggca tacgccgccg cggcggcaaa aaggaagtaa tcgatctccc gcgcacgcaa
    13801 caacgccgca cccagaaatc cgcaggcgtt cagggggaac aaggtgcgcc caatgctttc
    13861 gccccgccgg tcagtggtga ttctcagcag gtcgaagcct tcaaacaaaa tccagtacag
    13921 ccaaagcacc gggtggccca ccatggccca ttccggctgc gcctcgaggg cataaacgct
    13981 gtaggtgaaa acgagcccgg cgacagccat tcgggtccac tcaaatcgcc tggccacaaa
    14041 cagcagggcc gccgccagcg gcacagaagc cggaagcgaa aagcccggcg ccggagcgat
    14101 caccagcgtg acgaaaccga tgaagaaagc cagtccggta actacttcg
//
LOCUS       DASTMK010000221        11745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_619000, whole genome shotgun sequence.
ACCESSION   DASTMK010000221 DASTMK010000000
VERSION     DASTMK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619000"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFQ79_09880"
     CDS             <1..249
                     /locus_tag="VFQ79_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0140010.1"
                     /translation="GMKPFEVLRTGTRNVAEYFGTLKETGTVEQGKRADLILLDANPL
                     EDVTNVRRRAGVVVNGRWMAESEIQKRLSELAAAAEKM"
     gene            complement(261..1238)
                     /locus_tag="VFQ79_09885"
     CDS             complement(261..1238)
                     /locus_tag="VFQ79_09885"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0140011.1"
                     /translation="MERRRFLEASLLAAPMPASAQAPPAPREAQGGGTGSTSYQVQTT
                     VERRQTGKPHQGKVLAAIQPHCDDIPIFAGGTVLKLIDEGYTGYLITVTDDSMAGTGA
                     TYGEIVLKNEQDTNEVAKRLGCKDSIFLNYPNHNMDAMPVIEMRARFVFLFRALKVDT
                     VMVYDPSALYERNPDHYVTARAVEWAAAIAGTRWDYPEHFLAGLKPHAVRERYYFARG
                     PQLVNRVVDTSAFMDGKVFANMANVTQGPSGNTGARLRQRLAQQKKKLPLLGNDDETA
                     NREYTRHFALARDRVRGQAHSLEYAEYFHYIGPDESDVDAYVERNAVPL"
     gene            1335..1787
                     /locus_tag="VFQ79_09890"
     CDS             1335..1787
                     /locus_tag="VFQ79_09890"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0140012.1"
                     /translation="MKQRGNKRRAMLRAILAGGAGAGLLQAQAGSGWSPKKQSAGGPI
                     RSGHLLFLSGIGGWYPERRPKAGDIREQTADALTIMKTSLEKAGSSMENVLKVQVALV
                     DPEKNWEPMNEVYNTFFVKDRPARSYFGSTGFRRPGQLLQIDCIAYVD"
     gene            1806..3332
                     /locus_tag="VFQ79_09895"
     CDS             1806..3332
                     /locus_tag="VFQ79_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0140013.1"
                     /translation="MKGRAWSRRDAFRLSGGAAAGLMATRKPARAKNMSNIYTRIGVK
                     PFINLTATYTINGGALTLPEVKRAMDEASRYSVNLDELMEKVGARLAELMGAEAAIVT
                     SGCAAALAHSTAAAIAGADPEKMQRLPHLDGLRDQVIMPKQSRNAYDHAFRSPGATIV
                     EIDTAEEFHNALSDRVALVAVLGTGEPKGRIRLEEMVAAAHKRGIPVIVDAAAELPFT
                     PNPYLSRGADLVAYSGGKFLRGPQCAGILLGRKDLVQAAWLNSSPHHAFGRSMKVGKE
                     EIMGMLAAIEVWKNSYDLKAEYREWESWFQDIAGAVTKVAGVSTRILPPAGASPFPVM
                     EVSWDSARVGLTAGEVGQQLLDGEPRIMSHAEGEGHSFIIRPVAMQRGDHKAVAARLA
                     EILRQAPPVRTASAAVPARDISGNWRVTVEFIRGSAEHHLELSADGASVAGTHRATSL
                     TGRVRGTVDGSNVRLRSALPCEGTRLTYEFTGKVEGEHMSGEVGLGEYGRARWSAVRV
                     "
     gene            complement(3342..4382)
                     /locus_tag="VFQ79_09900"
     CDS             complement(3342..4382)
                     /locus_tag="VFQ79_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140014.1"
                     /translation="MRYSRRGFLRGSAAVLGSAPAIMGQKNSGGKRPITGSGEYRYEV
                     IHDWGELPANIRYGNTHGVCQDSQGRIYVHHTVNDASESHDTMVVFDDKEKFVKSWGK
                     EFKGGAHGLHIRKEGGTEFLYLCDTRRGIVVKTTLNGEEVFALGYPKESDAYKPDAEG
                     KPAKYSPTNLAIAPNGDIYVGDGYGSSYINQYNSKGEFLRTFGGKGSEPGKLNCPHGL
                     MVDTRGPEPLLLVADRTNKRLQYLTLTGQHVRFETGVNLPCHFDERQGMLVIPDLGAR
                     VTLMDRNNRVLTHLGDDSSGNWSELRKQSRDQFIPGKFICPHGACFDRDGNIFVVEWV
                     EVGRVTKLRRVA"
     gene            complement(4433..5701)
                     /locus_tag="VFQ79_09905"
     CDS             complement(4433..5701)
                     /locus_tag="VFQ79_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0140015.1"
                     /translation="MKSYALLLLSALIVHGAENQGPRQLADFGIAGDGKTDDTAAIQK
                     AVDAGIGELRFWRGTFRISKPVLIDLDRVGPVSISGAGVATIVMTGAGPAFRITGTHA
                     GNAAPSSIKPNVWSRQRAPMIDGIEIVGEHAGAIGIQLEGLMQPTLTRLVIRNALHGI
                     RITGTNRNIILSECHVYNNRGVGILLEDLNLHQINITGSHVSYNGGGGIVIRRSEIRN
                     IQIGTSDIEANMDVKGPATANVLFDAREGSILEGAITGCTIQHTRDAPGSANIRLLGR
                     SADDPNQVGNLIIANNVFSDVGVNIHLRHSRGVVVTANTFWKGYEHHMLVEGSSNIVV
                     GPNLLDRNPIYRTPDSPDNIVFTDSTDSSIDGLHITAPRNSEAALVLRRCRRFRLSNI
                     FILDAGNRGLLLDDTSDIEISGAGIGGRLP"
     gene            complement(5698..7074)
                     /locus_tag="VFQ79_09910"
     CDS             complement(5698..7074)
                     /locus_tag="VFQ79_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0140016.1"
                     /translation="MTRREFVGMACAGLAAPSRAAERPPNFLIIYADDLGIGDLGCYG
                     ARDVKTPHIDKLAAAGARFTDWYTNSPVCSPSRASLLTGKYPQRTGVTEVLASTAQFE
                     TRGLARGEITLPGELRKRGYRTGHIGKWHLGSAPESRPAVQGYDEFFGFYSGWADYYS
                     HRYYRQGRVPQEIFHDLWRNEREEFRDGEYHTEMFGAEAAAFLSRQQSGTPFFLTAAF
                     GAVHYPMMAPQKYLDRFSPQMDRERRMHAAVTAAMDDAVGLIVETLSRRGLLDNTVIF
                     FQSDNGATNEIRAHSRAMPYRGGSNAPFRGFKAGLFEGGIRMPAVLAWNGRIPGNRSV
                     SGVAGAMDIFPTFLNWAGVTQPYQVDGKNIAAMAQQGAPSPHDAFFWGYLKQRAVREG
                     PWKLILNPPSVPGDPVESDVWLSNLAEDPGEQVNLAKREPERTARLQKRIEDWFAGFD
                     SARSGVRA"
     gene            7199..8068
                     /locus_tag="VFQ79_09915"
     CDS             7199..8068
                     /locus_tag="VFQ79_09915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0140017.1"
                     /translation="MNRRRFLENTLGTAGAVAAGLANFPSPLAAAADKKATDIVHLGP
                     DKIRLTRLAIGSGTRGGRPQRELGVQGFADLLHFGYDNGLFFWETADSYKTHPHLKEA
                     LNRVPREKVTILTKTRARTAEEAKADLDRFRQELGTDYIDIVLLHALQAGNWPEERKG
                     AMEVLSEAREKGVIRTHGCSCHSLQALETASRTPWVRVDLARVNPAGLLMDSDPNTVV
                     NVLRKMKANGKGVIGMKILGEGGLRNRVDEALRFALSLDCVDCFTIGPENRNELADLL
                     KRIPAASQRAKAA"
     gene            8222..9838
                     /locus_tag="VFQ79_09920"
     CDS             8222..9838
                     /locus_tag="VFQ79_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0140018.1"
                     /translation="MDARHGGTRLNYSRLLREIRSGRRLIAGLKHQRGAAREHERLNR
                     LLVDNSLGLMCIHDLDGVLVAVNPAAARSLGFEMHEGAGRNLRDWLAPSVRNRFDQYL
                     SRIRNNAAASGLMRLVAKDGTERVWLYRNSKYESDGNPTLVVGHALDITDRVQAGRAL
                     KHGEERFRELFDYAPIAYHEIDRNGIIRRVNNAECDLLGKDSSELIGRPVWEQVVPEF
                     RENSRREVHRKLAGLQEIAPFVREYERKDGTRIIVEIHERLIYGARGDVQGMRSALID
                     ITARRKAEIRIQTLNAELEQRVEERTAALARSNDALKQFAYTVSHDLQEPLRSITGFA
                     GLLAARHSKALPEEGVEFLEQIADGAERMTSMIRSLLEYSRLLQSPVLTEPVNMKAVF
                     DAALSNLEAAIAESSAIITCGALPIAAVDPQLLMQVFQNLLSNSIKYCAARPPVIHVC
                     AQEAGGEWIFCVQDNGIGIDRANLGQVFGVFKRLHGREIPGSGIGLSICRAIIEGHGG
                     RIWAESDPGAGSKFYFTVNSYTGEHSEDSR"
     gene            complement(9850..10530)
                     /locus_tag="VFQ79_09925"
     CDS             complement(9850..10530)
                     /locus_tag="VFQ79_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140019.1"
                     /translation="MRSIGILLFASVCIAANTDGLKPRPSVTDYPARAVLPPMEIGAK
                     LLTPEEVRGAFVTGLDKDFLVIEVGLYPGAAKLDVDRDDFLLRVAGKDILRPADYKAL
                     ATAVQNANTASPSDVTIYPTVGVGYGTGPGGYGRGTSTSVGVGVGIGGAGGPRPASTD
                     RDRKTMETELSDKSLPEGPAAQPVAGYLYFPLPKKHRGVLYELEYVRGGDRARLNLGT
                     WGGDAKSR"
     gene            complement(10527..11051)
                     /locus_tag="VFQ79_09930"
     CDS             complement(10527..11051)
                     /locus_tag="VFQ79_09930"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEU0140020.1"
                     /translation="MRVLRLLIPGLGFALFITATPASEQPAAPEKNEESATVQRVKHR
                     RIRLGGINVSAGYSHHSGYWRPYWYPYGDPWYSPFSYGFYSPFYHPGHYYGFRQQDGM
                     GEVKLRSVDEDASVYLDGAYAGTAKDLKSMWLEPGAYNLEIRSDGTQQFSKRIYVLSG
                     KTLRLDTGGKDPRQ"
     gene            11168..11536
                     /locus_tag="VFQ79_09935"
     CDS             11168..11536
                     /locus_tag="VFQ79_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU0140021.1"
                     /translation="MSLHTIRVHNVTRQTILAEHAAVADTSSKRRTGLLKHDLLPAGE
                     GLWIVPCEGVHTFWMKFAIDIVYLSRKRKVVKVARRVAPWRVSICLRAHSVLELPAGT
                     IDATKTARGDMLEFTPVSGT"
BASE COUNT         2320 a         3443 c         3618 g         2364 t
ORIGIN      
        1 ggaatgaagc ccttcgaagt tctgcggaca ggcacgcgca acgtggcgga gtatttcgga
       61 acacttaaag aaaccggcac ggttgaacaa ggcaagcgcg ccgacctcat tctactcgat
      121 gccaatccat tggaggacgt gacgaacgtt cgccgccggg ccggtgtggt ggtcaacggc
      181 cgctggatgg cggagtccga aattcagaag cgtctgtcgg agcttgccgc cgccgcggag
      241 aagatgtagc accaactgga ctacagcggc accgcgttgc gctcgacata agcgtctacg
      301 tcggattcat ccggcccgat gtagtggaaa tactcggcgt actccaggct gtgcgcctgc
      361 ccgcgcacac ggtcccgtgc cagtgcgaag tgcctggtgt actcacgatt ggcggtctca
      421 tcgtcgttgc caagcagggg gagcttcttt ttctgctggg ccaggcgctg ccgcaaacgg
      481 gcgcctgtgt tgcccgatgg gccctgcgtc acatttgcca tgttagcgaa gactttgccg
      541 tccatgaatg cggaggtatc cacgacgcgg ttgaccagtt gcgggccacg tgcgaaatag
      601 taccgttctc tcaccgcgtg gggtttgagg ccggccagga aatgctcagg gtaatcccaa
      661 cgcgttccgg caatggccgc cgcccattcc actgcccgcg ccgtcacgta gtgatcgggg
      721 ttgcgctcat aaagcgccga gggatcgtag accatcaccg tgtccacctt gagagcgcgg
      781 aacagaaaca cgaatcgcgc gcgcatctcg atcactggca tcgcgtccat gttgtggttc
      841 gggtagttca gaaagatcga atctttgcag ccaaggcgct tggcgacctc gttggtgtcc
      901 tgctcgtttt tcagcacaat ttccccatag gtagcccctg tgcctgccat cgagtcatcc
      961 gtcacggtga tgagatagcc ggtgtagcct tcatcgatca gctttagaac cgtgccgccg
     1021 gcgaagatcg ggatatcgtc gcaatgcggc tgaatcgcag ccagcacttt gccctgatgc
     1081 ggcttgccgg tctgccgccg ttccacggta gtctgaacct ggtacgaagt gctgccggtt
     1141 ccaccgcctt gtgcctctcg cggggctggc ggcgcttggg cgcttgcggg cattggtgcg
     1201 gcgagcagag atgcttccag aaaacgtctt ctttccacac ttcctcctga gttggttttt
     1261 ctatggtatc cgcgattcga ttttcgtcaa tgggggattg cgcaaaatgg tgtgcggcgg
     1321 tataggatag accaatgaaa cagagaggca acaagcgccg ggccatgttg cgggccatcc
     1381 tggccggagg tgcgggagcg ggactgttgc aagcccaagc cggttccggg tggagcccaa
     1441 agaagcaatc ggccggagga ccgatccgtt ccggacatct gctgttcctg agcggtattg
     1501 gcggctggta tcccgagagg cggccgaaag cgggcgacat ccgcgagcag accgccgacg
     1561 cgctcaccat tatgaaaacc tcgctcgaaa aggccggatc ttccatggag aacgtactca
     1621 aggtgcaggt ggcgcttgtc gatccggaaa aaaattggga gccgatgaac gaggtctaca
     1681 acacgttttt cgtgaaagac cggcctgcgc ggtcgtattt cggcagcacg gggtttcgca
     1741 ggccaggcca attgctgcaa atcgactgca ttgcatatgt ggactgacga ggagggggtg
     1801 ccgttatgaa aggccgggca tggagccgca gggatgcctt tcgtctgagc gggggcgcgg
     1861 cggcggggct catggccacg cgcaaaccgg cgcgggcgaa gaacatgtcg aatatctaca
     1921 ccagaatcgg tgtgaagccg ttcatcaacc tgacggcaac ttacaccatt aacggcggag
     1981 cgctgacgct tcccgaggtc aagcgcgcaa tggacgaggc atcgcgctac tccgtgaacc
     2041 tcgacgagct gatggagaag gtgggagcgc ggctggcgga actgatgggt gcggaggcgg
     2101 ccattgtcac ctccggatgc gccgcggcgc tggcgcattc cacagccgcc gcgattgccg
     2161 gcgccgatcc ggaaaagatg cagcggctgc cgcatctcga cggtttgcgc gaccaggtga
     2221 tcatgccgaa gcaatcgaga aacgcctacg accatgcgtt tcgcagtccc ggcgcaacca
     2281 tcgtcgagat cgatacggcg gaagagttcc ataacgcgct aagcgatcgc gtggcgctgg
     2341 tggccgtgct cggcacggga gagccgaaag gccgtatccg cctggaggaa atggttgcgg
     2401 cggcgcacaa gcgcggcatc ccggtcatcg tcgatgcggc ggccgaactt ccgtttaccc
     2461 cgaacccgta tctcagccgg ggagcggact tggtggcgta cagcggcggc aaattcctgc
     2521 gtggaccgca atgcgcgggc attctgctcg ggcgcaaaga cctggtgcag gcggcctggc
     2581 tgaacagctc tccccatcac gccttcggcc ggtccatgaa agtgggcaag gaagaaatta
     2641 tgggtatgct cgccgccatt gaggtctgga agaacagcta cgatctcaaa gccgaatacc
     2701 gggagtggga aagctggttc caggacatcg ccggcgcggt tacgaaggtg gcaggagtca
     2761 gcacccgcat tctgcctccc gccggtgcga gcccgtttcc ggtgatggaa gtctcgtggg
     2821 attccgcacg cgtggggctt accgcgggag aagtcgggca gcaattactc gacggcgagc
     2881 ctcgaattat gtcgcatgcg gaaggcgaag ggcactcgtt tatcatccgg cctgtcgcga
     2941 tgcagcgggg cgatcacaaa gccgtcgcgg cgcggctcgc ggagattttg cggcaggctc
     3001 caccggtgcg cactgcgagc gctgccgtgc ccgcaaggga tatttccggg aactggcgcg
     3061 taacggtcga attcatccgg ggttcggcgg aacaccacct ggagctttcc gcggatggcg
     3121 cctcggtcgc gggcacgcat cgtgcaacgt ctctcaccgg acgtgtacga gggacggtgg
     3181 acggctccaa tgtacggctt cgcagcgcac tgccatgcga aggcacacgg ctgacctacg
     3241 aattcaccgg taaggtggaa ggcgagcaca tgtcgggcga agtcggcctc ggcgagtatg
     3301 gccgcgcccg atggtccgct gtcagggtgt gaaacggcgc ttcacgcgac gcggcgcagc
     3361 ttggtgacgc gtcccacttc cacccattcc acgacgaaga tattgccgtc gcggtcgaaa
     3421 caggcgccgt gcgggcagat gaactttcca ggaatgaatt gatcgcggga ctgtttccgc
     3481 aactcactcc agttgcctga tgaatcgtcg cccaggtgcg tgagcacgcg attgttgcga
     3541 tccatcagcg tcacgcgcgc accaaggtcg ggaatgacca gcatgccttg gcgttcatcg
     3601 aaatggcagg gcaggttcac gccggtctcg aaccgcacgt gctgcccggt gagcgtcagg
     3661 tattgcagcc gcttgttggt gcggtcggcg acgagcagca acggttccgg cccgcgcgta
     3721 tccaccatca gtccgtgcgg gcagttcagt ttgccgggct cggagccttt gcctccgaac
     3781 gtgcgaagga actcgccttt gctgttgtac tggttgatgt agctcgaacc atagccgtcg
     3841 ccgacgtaga tgtcgccatt cggagcgatg gccaggtttg tgggactgta cttggccggc
     3901 tttccctcgg catccggctt ataggcgtcc gattccttcg gatagccgag agcgaatacc
     3961 tcttcgccat tgagcgtcgt cttcactaca atgccgcggc gggtgtcgca caggtagagg
     4021 aattccgtgc cgccttcctt gcgaatatgg agcccgtgcg cgccgccttt gaactctttc
     4081 ccccaggact tgacgaattt ctctttgtcg tcgaaaacca ccatcgtgtc gtggctttcg
     4141 ctcgcatcgt tcaccgtgtg atggacgtag atgcggcctt gggagtcctg gcaaactccg
     4201 tgtgtgttcc cgtagcggat gttggccggc aactcgcccc agtcatggat cacttcataa
     4261 cgatattccc ccgatcccgt aatcggcctt ttcccaccag agtttttttg acccataatt
     4321 gccggcgccg aacccagaac ggcggcgctg ccgcgcagaa atcctctccg actgtaacgc
     4381 atgaaccagc cctccagctt tcttcagagt gtctcccact gtgggccggg cgtcaaggaa
     4441 gacggccgcc gatcccggct ccggagatct cgatgtcgga ggtgtcgtcc aacaggagcc
     4501 cgcggttgcc ggcatcgaga atgaaaatat tcgataaccg gaagcggcgg cagcgccgaa
     4561 gcacgagggc agcctcggag tttcgcggag cggtgatgtg aagaccgtcg atgctggagt
     4621 ccgtcgagtc cgtgaatacg atattgtcgg ggctgtcggg ggtgcgataa atcggattcc
     4681 ggtccagaag gttcgggcca actacgatat tcgagctgcc ttccaccagc atgtggtgct
     4741 cgtaaccttt ccagaaggtg ttggcagtaa cgaccacacc gcgagaatgc cggagatgaa
     4801 tgttcacgcc gacatcgctg aacacattgt tcgcgatgat cagatttcca acctggttgg
     4861 gatcgtccgc gctgcgcccc agcaggcgga tgttggccga tccgggcgcg tcgcgcgtgt
     4921 gctgaatcgt gcagccggtg atggcgcctt ccagaatcga gccctcccgg gcgtcgaaca
     4981 gcacgttcgc cgtagcaggg cctttcacat ccatgttcgc ttcgatatcg ctggttccaa
     5041 tctggatgtt gcggatctcc gagcgccgga tgacgatgcc gccgcccccg ttgtagctga
     5101 cgtgtgaccc cgtgatgttg atctggtgta gattcaggtc ttccagaaga attcccacac
     5161 cgcggttgtt atagacatgg cattccgaaa gaatgatgtt ccggtttgtg ccggtaatgc
     5221 ggattccatg cagcgcattt cgaatcacaa ggcgcgtcag tgtgggttgc atcaatcctt
     5281 cgagctgtat cccgatcgcc ccggcgtgct ctccaacaat ctcgattcca tcgatcatgg
     5341 gcgcccgctg ccggctccag acgtttggct tgatcgagct cggcgcggcg ttgcccgcgt
     5401 gtgtgccggt gatgcgaaac gcggggcccg cgcctgtcat aacgatggtc gcgacgcccg
     5461 cgccgctgat ggatacaggt cccacgcgat cgagatcaat caggaccggc ttgctgatcc
     5521 ggaacgtgcc ccgccaaaaa cgcagctcgc ctatcccagc gtccaccgct ttctgaatcg
     5581 ccgccgtgtc atcggtcttt ccatcgccgg caatgccgaa gtccgccaac tgccgcggcc
     5641 cctggttttc cgcgccgtgt acgatgagcg cactcaggag aagaagcgcg taagacttca
     5701 tgcccttact ccggagcgcg cggaatcgaa tccggcgaac caatcttcta tgcgtttttg
     5761 caggcgggcc gttctctcgg gttcgcgctt cgcaaggttt acctgttccc ccggatcttc
     5821 cgccaggttc gacaaccaca catcgctctc caccggatct cccggcacac tcggcggatt
     5881 caggattagc ttccacggtc cctcccgcac ggcacgctgc ttcagatacc cccagaagaa
     5941 cgcgtcatgc ggcgaaggcg cgccctgctg cgccatggct gctatatttt tcccgtccac
     6001 ctggtacggt tgggtcacgc ccgcccagtt gagaaacgtc ggaaaaatat ccatggcccc
     6061 ggcgactccg gaaacgctgc ggtttcccgg tattcggccg ttccaggcca ggacggcggg
     6121 catgcggatg ccgccttcga acaacccggc tttaaaaccg cggaacggtg cattgctgcc
     6181 gccgcggtag ggcatggctc gcgagtgcgc gcgaatctcg ttcgtggcgc cgttgtcgct
     6241 ctgaaagaag atgaccgtgt tgtcgagaag tccgcgcctg gacagagtct cgacgatcaa
     6301 ccccacggcg tcgtccatcg ccgccgtaac agccgcgtgc atgcggcgtt cacggtccat
     6361 ctgcggcgaa aagcggtcga gatacttttg cggcgccatc atcggataat ggaccgcgcc
     6421 gaaggctgcc gtgagaaaga acggtgtgcc ggattgctgc cttgacagaa acgccgcggc
     6481 ttcggccccg aacatctccg tgtgatattc gccatcgcga aactcttcgc gttcgttccg
     6541 ccagagatcg tgaaaaatct cctgcggcac tctgccctgg cggtagtagc ggtgggaata
     6601 gtagtcggcc catccggaat agaatccgaa gaactcgtca tagccttgca ccgccgggcg
     6661 gctttccggt gcgctgccga gatgccattt tccgatatgt ccggtcctgt agccgcgctt
     6721 acggagctcg ccgggcaacg tgatttcccc gcgcgcgagc ccgcgtgttt cgaattgcgc
     6781 ggtggaagcc agcacctccg tcactccggt tcgctgcgga tatttgccgg taagaagcga
     6841 tgcgcgcgag gggctgcaca ccggggagtt ggtgtaccaa tcggtaaatc gagcacccgc
     6901 ggccgccaac ttgtcgatgt gaggcgtttt gacgtccctc gctccgtagc accccaggtc
     6961 tccgatgcca aggtcatccg cataaatgat gaggaagttc ggcgggcgct ccgcggcacg
     7021 cgacggtgcg gctaatccgg cgcatgccat gccaacaaat tctcgtcgtg tcatcttgtc
     7081 ctatcttgca taaaaatacg gcgcgaaata cgcacccggc cgttgctcct gatggtctcc
     7141 caatgatact tgttaagaag ccggtttcag ggacccggcc gaggagccaa aaatgggaat
     7201 gaatcgccgt cgatttcttg agaacacact gggaactgct ggcgctgtcg ccgccggact
     7261 tgcaaatttt ccgtcgccgc tcgctgccgc ggcggacaag aaagccacgg acattgtcca
     7321 tctgggaccc gacaagattc ggctcacgcg cctcgctatc gggtcgggta cccgaggcgg
     7381 ccggcctcag cgcgaattgg gtgtccaggg tttcgccgac cttctccatt tcggctacga
     7441 caatggactg ttcttctggg aaacggcgga ctcctacaag acgcatccgc atctgaagga
     7501 agctctcaat cgcgttcctc gcgagaaagt cacgattctc accaaaacgc gcgccaggac
     7561 ggcggaggaa gccaaagccg atctcgaccg ctttcgccag gaactgggaa ccgactacat
     7621 cgatatcgtg ctgctccatg ccctgcaagc cggtaactgg cccgaagagc gcaaaggcgc
     7681 gatggaagtt ctgtcggagg cccgcgaaaa aggagtcatc cggacgcacg gctgttcctg
     7741 tcattcgctg caagcgctgg agacagcatc gagaacgccg tgggtgcgag tggacctggc
     7801 gcgtgtgaat ccggcaggat tgctgatgga ctccgatccg aataccgtgg tgaacgtcct
     7861 gcgcaaaatg aaagcaaacg gtaagggtgt aatcgggatg aagatcctgg gcgaaggcgg
     7921 cctgcgcaac cgcgtggatg aagcgcttcg cttcgctctt tcgctcgatt gcgtcgattg
     7981 tttcaccatc gggccggaga accggaacga actcgcggat ctgctgaagc gtattcccgc
     8041 tgcctcccag cgggcgaagg cggcctgacc ggcggaattg ccggactcat taggcggttg
     8101 atttctgcgt tccagcgaaa atcgcgccga aatatgctaa aatccggttc ataatcgcgg
     8161 gcctttttgc gctcgcacta cattcaccct cgccggcaat agtttttaga gcgaccggga
     8221 ggtggacgcg cggcacggag gaacgcgtct gaactacagc cgtctgcttc gggaaatccg
     8281 ttccggccgg agactcattg ctgggcttaa gcaccagcgg ggtgccgctc gtgagcacga
     8341 acggttgaac cgtctcctcg tcgataacag tctcggcctg atgtgcattc acgatctcga
     8401 cggcgtgctg gtggccgtga atcccgccgc cgcgcgctct ctgggttttg agatgcacga
     8461 aggtgccggc cgcaacctgc gcgattggct tgccccctca gtacggaacc ggttcgacca
     8521 gtatctctcc aggattcgca acaacgctgc cgcctccggg ctgatgcggt tggtggcgaa
     8581 ggacggtacc gaacgggtat ggctgtaccg caacagtaag tacgaatccg atggcaatcc
     8641 caccctcgtg gtcggacacg cgttggatat tacggatcgc gttcaggcgg gacgggcgct
     8701 caaacacggc gaggaacgct tccgggaact gttcgactat gctcccatcg cctatcacga
     8761 gatagaccgt aacgggatca tacggcgtgt caacaatgcc gaatgcgatc tgctcggcaa
     8821 agacagttcg gagttgatcg gcaggcccgt gtgggagcag gtcgtgcctg aatttcgcga
     8881 gaacagccgc cgggaggtgc atcgcaaact ggcgggattg caggaaattg ctccttttgt
     8941 ccgggaatac gaacgcaaag atggaacgcg gatcattgtt gagattcacg agcgcctgat
     9001 ttacggcgcg cggggagacg tgcagggcat gcgttcggca ttaatcgaca tcacggccag
     9061 gcggaaggcg gaaatccgta ttcagacgct gaatgcggaa ctggagcaaa gggtcgagga
     9121 acgaacggcc gcactcgcgc gctcgaatga cgctctgaag caattcgcct ataccgtatc
     9181 ccacgatctg caggagccgc taaggagtat taccggtttt gcgggtctgc tggcagcccg
     9241 ccattccaag gcgctgccgg aggagggcgt cgagttcctg gaacagatcg ccgacggggc
     9301 ggagcgaatg acatcgatga ttcgcagcct gctcgagtac tcccgcctgt tgcagtcgcc
     9361 ggtattgacg gaaccggtga atatgaaagc ggtattcgac gcggcattat cgaacctgga
     9421 ggccgctatc gcggaaagct ccgcgattat cacatgtggc gcactgccca ttgccgccgt
     9481 cgatccgcaa ctgctgatgc aggtgtttca gaatctgctg agtaactcca tcaagtactg
     9541 cgcggcaagg ccgccggtga ttcacgtatg cgcgcaggag gccggcggcg aatggatttt
     9601 ctgcgtacag gacaatggaa tagggattga tcgcgccaac ctcggacagg ttttcggagt
     9661 atttaagcgc cttcacgggc gcgagattcc aggttcgggt attggtttgt ccatctgccg
     9721 ggcgattatt gaagggcacg gaggcagaat ctgggcggag tccgacccgg gggcgggctc
     9781 gaagttttac tttacggtca actcgtatac cggagagcac tccgaagatt ctcgctgaag
     9841 tcagatccgt cagcggctct tcgcatcccc accccaggtt ccaaggttca gccgggcgcg
     9901 gtcgccgccg cgcacatact ccagctcgta gagcacccct ctgtgctttt tgggtaaggg
     9961 aaagtacagg tatccggcaa cgggttgtgc cgccggccct tccggcagag acttgtcgga
    10021 aagctccgtc tccatcgtct ttcgatcacg atccgttgat gccggacggg gaccgcccgc
    10081 gcccccgata cccactccca cgccgaccga tgtggatgtg ccgcggccgt agccgcccgg
    10141 gccggtacca taccccacgc caacggttgg ataaatcgtc acgtcggaag gagacgccgt
    10201 attggcgttc tgaacggccg tggcaagcgc cttgtaatcc gccggcctga gtatatcttt
    10261 gcccgccacg cgaagcagaa agtcgtcccg gtcaacgtcc aacttcgctg cgccggggta
    10321 gaggcctact tcaatgacga ggaagtcctt gtcgagtccc gtcacgaaag cgccgcggac
    10381 ctcttccggc gtcaggagct tggccccgat ttccatcgga gggagaacag cgcgggccgg
    10441 ataatcggta acgctggggc gaggcttcag tccgtctgta ttggcggcga tacaaaccga
    10501 tgcgaacaac aggattccga tcgatctcat tgccgtgggt ccttcccgcc tgtatccagg
    10561 cgcaaggttt tgccgcttaa aacataaatt cgtttgctga attgctgcgt accatcagac
    10621 cgtatttcga ggttataagc tccgggctcg agccacatac tcttcaggtc tttcgcggtt
    10681 cccgcgtatg ctccatccag gtacacactg gcatcctcgt caaccgagcg aagcttcact
    10741 tctcccatgc catcctgctg ccggaatccg taatagtgcc cagggtgata aaacggcgag
    10801 taaaagccgt aagaaaacgg agaataccag gggtctccat agggatacca atagggccgc
    10861 caataaccgg aatgatgcga gtagccggcg ctcacgttta tgccgccgag gcggatacgc
    10921 ctgtgcttca cccgctgcac ggttgcggat tcttcgtttt tttccggggc agccggttgc
    10981 tccgaagccg gcgtggcggt tataaaaaga gcgaagccga gaccaggaat caacaggcga
    11041 agaacgcgca taaatcctcc cttcgcggag tatatattcg acgtctccag cgggtggctt
    11101 ggtttcagta aagccgggcc gtaaaattgc tccgctggtt taatttcgat acgctgagcg
    11161 tttcaaattg tcattgcata ccattcgagt tcacaatgtc acgcgccaga ctattctggc
    11221 agaacacgcc gctgttgcgg acaccagcag caagcgccgc acgggcttgc tgaaacacga
    11281 tcttctgcct gccggcgaag gattgtggat tgtcccctgt gagggagttc atacgttttg
    11341 gatgaagttc gccatcgaca tcgtatacct gagccggaaa cgtaaggtcg tgaaggtggc
    11401 ccggcgagtc gcgccatggc gcgtttcgat ttgcctccgc gcccattccg tactggaact
    11461 gccagccggt acgattgacg cgacgaagac ggcgcgcggc gatatgctgg aattcacgcc
    11521 ggtgagcggc acttgagagc ttgttgaaaa atgcagaaaa ccgcttgcgg acgcgcgcgg
    11581 ctccgcttct gagccgcgac cgcgagggag cggtggctgc aattataaag ggtcttaacc
    11641 atgcgcaatt tgagagaagg cagcagcggc ccggacgtcg tacgcctgca ggaaaggctc
    11701 aaggaactcg gcttcaatcc gggtgcgacc gacgggcagt ttgat
//
LOCUS       DASTMK010000222         7956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_170767, whole genome shotgun sequence.
ACCESSION   DASTMK010000222 DASTMK010000000
VERSION     DASTMK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170767"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VFQ79_09940"
     CDS             complement(<1..1471)
                     /locus_tag="VFQ79_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140022.1"
                     /translation="MYLGVALTTLAALLLELSLTRLFSVVFYYHFAFLAISIALFGFG
                     AGGVFSYVAIGWRGHFYSKLGWLASANCMAVLLALVFVLTRNGEPGYGTLALVYLFSA
                     LPFFFSGITVSLAIAESISRVDRVYFFDLIGAAAGCVLFVPFLNVIGGPNTVIATAVL
                     FAASSAIWHNLAGSTRGRIIAVGLALALVALVVVNTKSPLIDVRYAKGQALENERFVR
                     WNSFSRVALAPEKGSGQMSIIIDADAATGIANFDIDNLTREQRRELLHQGPGFPYVIR
                     PAAKTLIIGPGGGWDVARALASGSKDVTGVEINPIIATTIMRQRFPELSRRLYFRPEV
                     KIVIEDGRSFIRRSTGKYEVLQATLVDTWASTAAGAFALSENNLYTTEAFYEYLSHLT
                     GNGVLAFSRWGFDPPRESLRLLSLARAALARLGEHDLWRHVMVVREGDVNGWGSLDTV
                     LISREPFAPSDLARATEALAMSKLQRVYLPGDGAVNLFGQ"
     gene            complement(1699..2172)
                     /locus_tag="VFQ79_09945"
     CDS             complement(1699..2172)
                     /locus_tag="VFQ79_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140023.1"
                     /translation="MPENTYSRTGVDEPVALLAITSNGESLSRFREIAASSKWKLTSA
                     CGCAEARQIFEKECIPIIVTDTALSDGHWRDVLETCAKLPESPLLIVTSLHADELLWA
                     EVLNLGGYDVLMQPFDRAEVTRVIELAYAQWTRRNTRRTASKESPADTAGLPASA"
     gene            complement(2260..3294)
                     /locus_tag="VFQ79_09950"
     CDS             complement(2260..3294)
                     /locus_tag="VFQ79_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0140024.1"
                     /translation="MPRVEKAVYPYSVVPGGVWSKEDVARAAQSDTVVADHYAEIDAD
                     HIRPVTVVKPARAYVSYRKNGKIYWSSRRISLHQGETLLTDGSQCVRGRCGNRLSEVP
                     REPALPPAQEPDETALNSNPVLIADAPSANEPVFEFPTTGMRIPVLPRPALISEIASA
                     APASAILQPAREGSAGADPGWYLPGPAGIAPLLLARGSTPGGGTDPVTPPTVQPPEVI
                     IDPENPPDKPTPGGPDPGGEPQDPLPFIPLPPRPPVPPPFVVPPNVIVPPEIIIPPDE
                     PEIPVVPPDGPEPPGPPGPPGPPEISETPEPATLLTIPAGLGLLILRKRYSRRKTGQE
                     NRMRSSKTLD"
     gene            complement(3690..5942)
                     /locus_tag="VFQ79_09955"
     CDS             complement(3690..5942)
                     /locus_tag="VFQ79_09955"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0140025.1"
                     /translation="MMPRKLPFTRREWLALAAALPLAGQEQAGQTLPWPASHPGDIPS
                     GWNVLHGHPEFVHAEGMSGAHVGRLLDAFEEERARVIRSAKTPDQAQKYQAEARAMLI
                     ETIGKLPPRTPLRARLAGTLDRGEYVIEKVIFESRPRYYVTANVYVPRAARPPFPAVL
                     ASVGHWGAGKAFPDYQRLGAYFASRGFLVMVFDLPGMGERVESWDTVSRRPIVHPGTS
                     EYFVTTEHGIAAGRTILTSGNLVTYLIWDAVRALDYLAARDDVDAARIAATGVSGGGW
                     QTELLAAFDPRVKAAMPACYGGCIADLLFGAKISTADVDALIAPRPLLLMEATGDSRL
                     SVMEKLRRRKLVGDFYRAAGAGDRLRFMLAEGPHGYTSSMFAVMLDWLVRWLPAPTPP
                     AAQTAERPLQLESESELACTMTGQTVTALGGETLFSLNRTEARRLAAQWPAPRQSADL
                     EGWRGQLRKRVLERLAMPERDQAVKSSVLDRNDRHTYSVERLVYYSEPEVYVPSLLLL
                     PRRNGPAPAIMVVNETGKSAEGLAETHLVPLCEAGHVVFSIDPRGMGETAIARQRADY
                     AGLAVGEDAGLFYRALRSRRTMVGMRVFDVMRAVDYLQTRPEVDSGRISAIGMGAGGP
                     LVLFAAALENRIRRVAVSGAPVSYMAIVESRIFTHALSNFVPGALQHFDLPQAAALLA
                     PRPLLIVNPVDGRHRRVDEEQAAGTYKFTSRVYEMAGSGEAFGLAMADTAGELLKLWS
                     EHIRPLAGTA"
     gene            complement(5962..>7956)
                     /locus_tag="VFQ79_09960"
     CDS             complement(5962..>7956)
                     /locus_tag="VFQ79_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140026.1"
                     /translation="GVQRRQSKAGVFSWNHTISPTMVNEFRFGASYHNNPYWIEAVGS
                     DILKDVGIQGISTGPYNTAPVFYITGVSATPWEDSLYNPVTNYQWIDNVSITRGKHLM
                     KFGVDAGRVQVNGESTRPETYGAYTFSGIYSGFGYADFLLGIPQTTSVTIPNPPRDFR
                     GTTLGIYAQDQYKVNRSLTLNFGIRYELAKPYSSKHGTMYSFDPRTGSLVIPDKGVEK
                     LNPLYPRNIEVITASAAGFPANLLETHHLFYPRVGFAYKLFGGEKSVIRGGYGIYGNA
                     IHTALGAQGMTGGPFSGSVTYNNAIANGVPLFSFPSPFLSSGSTSTQNVNGFNPSLKQ
                     PYTQQWNLTLEQQIATVGLRVAYLGTRSVHLLYRRNLNQPPASTTPYSVSRRFYPVYN
                     QIIYTDSGGTQSYNALEVAADKKYGNNLTFSTGWTWAKDLTDTLDAGSPWSGQVIQDQ
                     FDRNVERTNNSTTRPHRIFAYSLYTLPFGKGQRFLSNARRPVRHILGGWELGAIMVIQ
                     SGQYFTPSFSGFDPSNTQTFGGRPDRIGNGNLDSGRSIGRWFDTSAFAIPGCPADRPV
                     CTNPAAVGRFGNSAQNILAGPRLIGLDATLMKAFDLGERFRLNLRLNVANALNHPSFN
                     LPNANITSVGTVGTITGQSRGALGGQTGPREVDLGLRLEF"
BASE COUNT         1592 a         2401 c         2441 g         1522 t
ORIGIN      
        1 attggccgaa aaggttcacc gctccatcgc cgggcagata aacgcgctgg agtttcgaca
       61 tggcaagcgc ttcggtggct cgcgcaagat ccgacggcgc gaacggttct cttgaaatca
      121 acactgtatc gagcgaaccc cagccgttca cgtccccttc gcgcaccacc atgacgtgac
      181 gccacagatc gtgttcgccg agacgtgcca gtgcggcccg ggccagcgac aggagccgca
      241 gggattcccg cggcggatcg aaaccccagc gcgagaacgc gagcacgcca ttccctgtca
      301 ggtggctcag atattcgtaa aaagcctcgg tggtgtaaag attgttctcc gacaatgcga
      361 acgcacctgc cgccgttgaa gcccaggtgt ccaccagtgt cgcctgtaac acctcatact
      421 tgccggtgct gcggcggatg aagctcctgc cgtcctcgat tacgattttt acctcgggcc
      481 tgaagtacag ccggcggctg agttccggga atcgctgccg catgatcgta gtcgcgataa
      541 tcgggttgat ctcaaccccg gttacatcct tactgccgga cgccagggcg cgagcgacgt
      601 cccatccgcc gcccggtccg ataataagcg tcttggccgc cgggcgaatg acgtacggaa
      661 accccgggcc ttgatgcagc agttccctgc gttgctcccg ggtcaggttg tcgatatcga
      721 aattggcgat tcccgtggcg gcatcggcat cgataataat gctcatctgt ccggaaccct
      781 tctccggcgc cagcgccacc ctggagaagc tgttccagcg gacaaagcgc tcgttctcga
      841 gtgcctgtcc ctttgcatag cgcacgtcga tcaaagggct cttggtattc actacgacca
      901 gcgccaccag cgcaagggcc agtccgacgg cgatgatccg gccgcgcgtg ctgcccgcca
      961 ggttgtgcca aatggcggag gatgcagcga agagcaccgc ggtggcaatc accgtgttgg
     1021 gcccgccaat cacgttcagg aacggcacaa ataacacgca gccggctgcc gctccaatca
     1081 aatcgaaaaa atacacgcga tcaacgcgcg agatggattc ggcgatagcc agggaaaccg
     1141 tgatccccga aaagaagaat ggcagcgcac tgaacaaata gaccagcgcc agcgtcccgt
     1201 agccgggctc tccgtttctg gtgagcacaa aaaccagagc cagcagtaca gccatgcaat
     1261 tagcggaagc aagccagccg agtttgctat agaaatgacc gcgccagccg atggcaacgt
     1321 aggagaaaac gccgccggct ccgaacccga aaagcgcgat ggagattgcc agaaaggcaa
     1381 aatggtagta gaagacgacc gaaaaaagcc tggttagcga caactcgagc aggagcgccg
     1441 cgagagtggt gagcgcgaca cccaagtaca cttgaggcca atgcagcccc tctctgctcg
     1501 tggatgaaga aattcgagac aaataactat gttagccaat cggggagacg gcactcacgc
     1561 agccggccgc cccggcacgt ggcagatcga tgtcagaggt ggccggctgt ctcttccccc
     1621 gctcgaacag acgtattccc cgcacaccgt gtttctttga tatcggctat atcgaaggtc
     1681 tcgggttggg agcgcggttc aagcggaggc gggcaggccc gccgtatcgg cgggactctc
     1741 tttggaggcg gttcgccggg tgttccgccg ggtccactgc gcataggcaa gttcaatcac
     1801 gcgagtcact tccgcgcgat cgaacggctg catgaggaca tcgtatccgc cgaggttgag
     1861 gacctccgcc cagagcaact catcggcatg cagcgacgtc acgatgagca gtggcgattc
     1921 gggtagcttc gcgcaggtct ccagcacatc gcgccaatgg ccgtcactca acgccgtatc
     1981 tgtcacgatt atgggaatac actctttttc gaagatttgc cgggcttcgg cgcagccgca
     2041 cgcagaagtc agtttccact ttgaggacgc ggcaatctca cggaagcggg agaggctttc
     2101 cccgttcgag gtaatggcga gcagagcgac gggttcatca acgccggtac gtgaatacgt
     2161 attttcaggc atttaagaat cgtagacgct aaaaactaac gccaaattcc aaaaaattcg
     2221 aggtcagcgc cctgattgat catggcgctc cgtgtacggt tagtcaagag ttttcgaact
     2281 gcgcatgcgg ttctcttgtc cggttttcct tctcgagtag cgcttccgta gaatcagcag
     2341 ccccagcccc gcggggattg tgagcagcgt agccggttcc ggagtctccg atatctcggg
     2401 cggaccggga ggcccgggag gaccgggggg ttcgggtccg tcgggtggta cgacgggtat
     2461 ctccggctca tcgggcggaa tgataatttc cggcggaaca atcacattgg gcggcactac
     2521 gaaaggtggt ggcacgggcg ggcgcggtgg cagcggaata aagggaagag gatcttgtgg
     2581 ttctcctccc ggatccggcc cgccaggcgt cggtttatcc ggaggatttt ccggatctat
     2641 gatcacctcc ggtggttgca ctgtcggtgg agttacgggg tctgtgccgc ctccaggtgt
     2701 ggaaccgcgc gccagcagga gcggagcgat gcccgcgggc cccggcagat accatcccgg
     2761 gtcggcgccg gcgctcccct cgcgagcggg ttgcaaaatt gcgcttgccg gagcggccga
     2821 tgcgatctcg gagatcaacg ccgggcgtgg aagcaccggg atgcgcatcc ccgttgtggg
     2881 aaactcgaaa acaggctcat tggccgacgg agcatccgcg atcagcaccg ggttggagtt
     2941 caaggccgtt tcatcgggct cttgggcggg cgggagtgcc ggttctcgcg gcacctccga
     3001 caggcggttg ccgcaccggc ctcgcacaca ctggctgccg tcggtcaaaa gagtctcgcc
     3061 ctggtgcagg ctgattctgc gtgagctcca gtagattttc ccgttttttc tgtaggatac
     3121 gtaggcgcgt gcgggtttca cgaccgtgac cggccggatg tggtccgcat cgatttccgc
     3181 ataatgatcc gcgaccacgg tatcggattg cgcggcccgg gctacatcct ctttcgacca
     3241 cacgccgccg ggaaccaccg aatacggata caccgctttc tcgacacgcg gcatccgcgc
     3301 aggttccgct gtttccgata atgagagctc cttcacccca tggtgcctgg agcaggaaat
     3361 agcgaagaat gccgtacagg atagggccgc acacacaaac ccctgcgctg tccgatggag
     3421 tctgatctgg cccatttcaa ccggctagta tttgaatccg aatgttcaca ctgaagagtc
     3481 tgaagcgaaa cggacggaag gaaaataggc gggcggtaac aggcctgctc gggtccgtac
     3541 ccgtacttca ggtcacatct gtttagtagc gcgaaaagaa aattatatcc caaatagacc
     3601 gtaatatcct ataactttat tttggggtag ctggtcactt tggaatgatg ctgattccgc
     3661 cgctgcgttc caggacagcg tacttgattt taggcggtgc cggcgagtgg acggatatgt
     3721 tctgaccaca gtttcagcaa ctcgccggca gtatccgcca ttgccagccc gaacgcttca
     3781 ccggaacccg ccatctcgta tacccggctg gtgaacttgt acgttccggc tgcctgctcc
     3841 tcgtccaccc tgcggtgccg tccgtccacg gggttgacga tgagcagcgg tctgggcgcc
     3901 agcagtgcgg ctgcctgcgg cagatcgaaa tgctgtagcg cccccggcac gaaattgctc
     3961 agcgcatgcg tgaaaatcct gctttccaca atggccatat aggaaacggg cgcgcccgat
     4021 accgcgaccc gccgaatccg gttctcgagt gctgcggcaa agaggaccag cggtccgccg
     4081 gcacccatcc caattgcgga aatcctgcca ctatccactt ccgggcgggt ctgcagataa
     4141 tcgacagccc gcatcacgtc gaagacacgc attcccacca tggtgcggcg cgacctaagc
     4201 gcccggtaaa acaagccggc atcttcaccg acggccaagc ccgcgtagtc ggcgcgctgc
     4261 ctggctatag cggtctcccc catgccgcgc ggatcgatgg aaaagaccac gtgacccgcc
     4321 tcgcacagcg gcacaagatg ggtttcagcc aatccctccg cgctcttccc cgtttcattt
     4381 acgaccatga tcgcgggtgc gggcccgttc cgcctgggca ggagtaaaag cgagggaacg
     4441 tagacttcgg gctcgctgta gtaaacgagg cgctcgacgg aatacgtgtg cctgtcattc
     4501 cggtccagaa ccgacgattt gacagcctgg tcgcgttccg gcatcgcgag ccgctccaga
     4561 acccgcttcc ggagctggcc acgccagccc tccaggtccg cggactgccg cggggccggc
     4621 cactgggcag ccaaccggcg ggcttcagtg cggtttagag agaagagggt ctcaccgccc
     4681 agcgccgtga cagtctgacc cgtcatggtg caagccagtt ccgattcgct ttcgagttgc
     4741 agcggacgtt ccgcggtctg cgccgccggg ggcgtgggcg ccggaagcca gcgcaccagc
     4801 cagtcgagca tgacggcgaa catggaagac gtgtagccgt gggggccctc ggcgagcatg
     4861 aaacgcaggc ggtctcccgc cccggccgca cgatagaaat cgcccacgag tttccgcctc
     4921 cgcagctttt ccatcaccga gaggcgggaa tcaccggttg cctccatcag gagcagaggg
     4981 cgaggcgcga tgagggcgtc gacgtccgcg gtcgagattt tcgctccgaa cagcaagtca
     5041 gcgatgcaac cgccgtaaca ggcgggcatt gcggccttga cgcgcggatc gaaggcagcc
     5101 agcagttcgg tttgccagcc gccccccgat actccggtcg ctgcgatcct ggccgcatcg
     5161 acatcgtccc gcgcggccag gtaatccaga gcccggacgg catcccaaat caggtacgtc
     5221 accaggttgc ccgaagtcag gatggtgcgg ccggcggcga ttccatgctc ggtggtcacg
     5281 aagtactcag aggttccggg atggacgatg ggacggcgag agacggtgtc ccatgactcg
     5341 acccgctcgc ccattcccgg cagatcgaag accatcacga gaaacccacg gctggcgaaa
     5401 taagcgccca ggcgctggta atcgggaaac gccttgcccg cgccccaatg gccgacactg
     5461 gcaagcacgg cgggaaacgg cggccgtgcc gcacgcggca cgtacacgtt ggccgtcaca
     5521 tagtatcgcg gccggctctc gaagatcact ttctcgataa cgtattcgcc gcggtccagc
     5581 gtgcccgcca gcctggcccg gaggggtgtg cgcggcggca gtttcccgat ggtctcaatc
     5641 agcatcgcgc gggcttcggc ttggtatttc tgcgcctgat cgggcgtttt ggccgacctt
     5701 attacccgcg ccctctcctc ctcgaaggca tcgagcaacc ttcctacgtg ggcgccggac
     5761 atgccttcgg catgaacaaa ctccggatgc ccgtgtaaca cgttccaccc ggacggaata
     5821 tcgcccgggt gagaggctgg ccacggcagg gtttgcccgg cttgctcctg acccgccagc
     5881 ggaagagccg ccgccaatgc cagccattct ctccgtgtaa agggcagctt tcgtggcatc
     5941 atgctttaaa cgaactccgc gctaaaactc caggcggagt ccgagatcga cttcccgagg
     6001 ccctgtctgg ccgcccaggg cgccacggct ttggccggtg atcgtgccca ccgtacccac
     6061 agaggtgata ttcgcgttcg gcaggttgaa actcggatgg ttgagcgcat ttgccacgtt
     6121 gagcctgaga ttgaggcgga atctctcgcc caggtcgaac gctttcataa gggtggcgtc
     6181 gagcccgata aggcggggac cggccagaat gttctgcgcc gagttgccga acctcccgac
     6241 tgccgccggg ttcgtacaca cgggcctgtc cgcgggacag ccgggaatcg caaacgcgct
     6301 ggtgtcaaac cagcggccga tggatcgccc ggaatcgaga ttgccgttcc cgatccggtc
     6361 cggccgtcct ccgaacgtct gggtgttcga gggatcgaat ccggagaacg aaggcgtgaa
     6421 gtattgacca gactggatca ccatgatcgc accgagttcc cacccgccga gtatatgccg
     6481 gacgggccgt ctggcattgg aaaggaagcg ctgtcccttc ccgaacggca gggtgtatag
     6541 cgaataggcg aagatccgat gcgggcgggt tgtcgaattg ttggtccgct cgacattgcg
     6601 gtcgaactga tcttgaatca cctggcccga ccacggcgag cccgcgtcca gcgtatccgt
     6661 caaatccttc gcccaagtcc agcctgtgct gaacgtcagg ttgttcccgt atttcttatc
     6721 ggccgcgacc tcaagcgcat tgtaggactg ggtgccgccg ctgtcggtgt agatgatctg
     6781 gttgtacacg ggatagaacc gccgggaaac ggaataggga gtggtgctgg ccgggggttg
     6841 gttaaggttg cgcctgtaaa ggaggtgcac gctcctggtt cccaggtagg ccactcgcaa
     6901 tccgacagtc gcgatctgtt gttcgagggt caggttccac tgctgggtat acggctgctt
     6961 caggcttgga ttgaaaccgt tgacgttctg cgtcgaggtc gagccggagc tcagaaacgg
     7021 cgaagggaag ctgaaaagcg gcaccccgtt agcaatggca ttgttataag tcacggagcc
     7081 ggagaacggc cctccggtca taccctgggc gccgagcgcc gtgtggatcg cgttgccgta
     7141 aatgccatac ccaccccgga ttacggactt ctcgccaccg aaaagcttgt aggcaaagcc
     7201 caccctggga tagaagagat ggtgtgtctc cagcaggttc gccgggaagc ccgccgcgga
     7261 agccgtgatg acctctatgt tccgcggata cagcggattg agcttctcca cacccttgtc
     7321 cgggatcact agcgatccgg tccgcggatc gaagctgtac atagtgccgt gcttgctgct
     7381 gtacggcttc gccagttcgt agcgtatgcc gaagttcagg gtgaggctgc ggttcacttt
     7441 gtactggtct tgcgcgtaaa tgcccagcgt ggtgccccgg aagtcgcgtg gtggattggg
     7501 gatcgtcacc gatgtcgtct gcgggattcc gagcaggaaa tccgcatagc cgaagccgga
     7561 atagatgccg ctgaaggtgt atgctccgta tgtctccggt ctggtggatt ccccgttaac
     7621 ctgaacccgg ccggcatcca ccccgaattt cataagatgt ttgccgcgcg tgatgctcac
     7681 gttatcgatc cactggtagt tggttacggg attatagagc gaatcctccc agggtgtcgc
     7741 gctcaccccg gtgatgtaaa acaccggtgc ggtgttgtag gggccggtgg aaataccctg
     7801 aattccgaca tctttcagaa tgtcgctgcc taccgcctca atccagtacg ggttgttgtg
     7861 gtatgaggcg ccgaaccgga actcgttcac catggtggga gagatcgtgt ggttccagga
     7921 gaagacaccg gctttgctct gcctcctctg aacgcc
//
LOCUS       DASTMK010000223         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_202522, whole genome shotgun sequence.
ACCESSION   DASTMK010000223 DASTMK010000000
VERSION     DASTMK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202522"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VFQ79_09965"
     CDS             <1..1160
                     /locus_tag="VFQ79_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0140027.1"
                     /translation="VAGFLKGIGFETSMYSLEDVPRLAEHPLYWKGRDYKDRPNVGAR
                     RRGRGRGRSLVLSGHIDTVPAGTQPWTKNPFGGEIEGNRLYGRGSNDMKCGIATNLFV
                     AEAVCEFGIDLGGGLIVESVVDEEFGGVNGTLAGRLMGYNADAAIISEPSLLRICPAQ
                     RGGRTVHLTLNTGPSGGILTGGRLAAGVAEQLRHFLVRVRDFAQQRRERASSHPLYPR
                     HTDPVPVAITKVWTGPWGTGEPVSVPETCMVEMYWQAMPGEKLEDIDREFHAWLDSVV
                     DSAPDLFPERPRAEFPIRWLPGSAISKGEPLVAEFAACATELLGAEPVIAGIEGPCDM
                     YIFHQQFGIPAILWGAKGANTHAADEYVEIDSVIDAARVLLSFIYRWCGAN"
     gene            1164..1382
                     /locus_tag="VFQ79_09970"
     CDS             1164..1382
                     /locus_tag="VFQ79_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEU0140028.1"
                     /translation="MTGLEMHSVVALLEDLPEEGLVRGQVGAVVETWAPGVYEVEFCD
                     DNGRSYAMVALKADQLMRLHHGRVHRAA"
     gene            1636..4914
                     /locus_tag="VFQ79_09975"
     CDS             1636..4914
                     /locus_tag="VFQ79_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140029.1"
                     /translation="MRIFVCLLCLLALAAGFALSQSTNATITGTVTDSSGAVIPAAKI
                     TVTNIDTNLEVIGSTNEVGNYSLPQLREGRYILTVEAAGFQPFTARDVVLTSRDVRRI
                     DAVLQLGQTATAIEVSAGATLIETETARISNTQTADMLKSVPLNARWLWAFFNITPGY
                     VNAGEGYRFAGAQASQVNWAIDGTTFSDNVGGGSAIGPLANYVESFQEVKIDMANNSA
                     EFGTLGQVTVISKSGGNSLHGSAADYLHTPWFRARNPFANARTPGVYHIYAGSIGGPV
                     YLPKIYDGRNKTFFYTVYEGSIGGDTATMLNPTVPLESWRKGDFSSLLPGTAIYDPTT
                     GQPFANNQIPASRINPVSQKLQDRYYPLPNFGDTSSLLASNYREMKSRGWDIPKYWVV
                     RGDHHLSAKDLIFGRFTFTRGPVTPWEGNLPTIGQRIQRRDTRAASASWTRTINAQMV
                     NEVRWGMNLNNNPLQGPVNGLEQARELGLQGLAPDLPDVGGMLKIRWQNIGLTPLQQI
                     DWTNPGYRNHNQEFQEHFSWFRGRHNLRMGFDLTRAEWDERAANDALFGALTFSSQYT
                     SNGLSGQGHPYADFLLGIPTTAARAYPPVRSDRNRWNYDFFITDEWKVNQKLTLTIGA
                     RYELHKPWRENNDFISLFDIGTGSVVVPDGALSKISPLYPADFAPVIEAKDAGLPGRT
                     LIRTDRNNIAPRIGIAYRPFSQDTVVRAGYGIYYDPVPRNLTMGGMPFVLREEPYTNP
                     LTNPTVTLPRVYPDGATTRATGLSLPAAVNPDLRFPYSQQWNLTIERQQWDTGFRVSY
                     IGTNTRQIDWAYNINSPVPDSRLFVEKARLYPQYSGISYYTNGANHQYHALTTAVERR
                     MKNGLMLQSSWTWARDIYDLTSGQMPENPFDRERERAVSRSIPTHRVVNSYVYQLPFG
                     KGQKWGSNLPKYADMAVGGWALSGVLTLQTGQFLTPVWSGPDPVGIAFTGSTTPASVT
                     LRPNVLRDPNLPVDQRTPGRWFDVSAFGPPAKGSFGSAAKGIIEGPGINSWNMGLQKD
                     LMFGERLPRINLEITAVNMFNRPNYGNPGMNISQAASAGVITGLIGDDYRAREFRAGL
                     RVEW"
     gene            complement(4968..>6467)
                     /locus_tag="VFQ79_09980"
     CDS             complement(4968..>6467)
                     /locus_tag="VFQ79_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140030.1"
                     /translation="TDSSQKVRRIRPDGIIETVAGTGEYGSSGDGGPATNATLQSVRG
                     LAVDARGRLFISDYTANRVRVVETDGTIRTYAGTGERVRGGEGTPAVSSPIYDPSQIT
                     ADTHGALTINEGTTILRRITPDGVMTTIGPTIPFGRPGDDGARQCIFSNLWGLAGLEH
                     DTDGSLLVSGSNRICRLMPDGTSWQVAGTHFFGFSGDGGPAASATLWAPQRIAVDPKT
                     ETIYVADAGNHRVRRLQPDYPPGAFPKAPYIAAVIGAGASAAPVTRLSPGGLASVFGT
                     DFAPPGTYAAVQSSDFVAGVLPTKLANTCVEVGGKRAFLTYAGTAQINFQVPDIPVDT
                     DVSVQVVANCGTATEIRSAGWMVEARITAPEFLYWVHGTNGRSPVVAVDATTGAKIGP
                     PGLIAGLAFRPAKPGDILTIYCISLGPANPPVQAGAGASGEARITTALPVAIDGKPVP
                     AENILYAGLSPDSPGLYQINLVLPALSDGDHPITIGDSPAEGFIRVGNP"
BASE COUNT         1311 a         1969 c         1910 g         1277 t
ORIGIN      
        1 acgtcgcggg attcctcaaa ggcatcggat tcgagacctc catgtattcg ctcgaggacg
       61 tgcccagact cgcagagcac ccgttgtatt ggaaagggcg cgattacaag gaccgcccga
      121 acgtgggtgc ccggcgccgg ggccgcggcc gcggccggtc tcttgttttg tccggccaca
      181 tcgacacggt tcccgcgggc acgcagccat ggaccaaaaa tccttttggc ggcgaaatcg
      241 agggaaaccg cctctatggg cgtgggtcca acgatatgaa gtgcgggatc gccaccaatc
      301 tgtttgtggc ggaggcggtg tgcgaattcg gaatcgatct gggaggcggc ctcatagtgg
      361 agagcgtggt ggatgaggag ttcggtggcg ttaacggaac ccttgcgggc cgtctgatgg
      421 gctataacgc cgacgcggcc atcatctccg aaccttcgct gctacgaatc tgcccggcgc
      481 aacgtggcgg acgcacggtt catctcacgc tcaacaccgg gccaagtgga ggcattctca
      541 cgggaggccg gctggcggcc ggtgtggccg aacaactgcg ccactttttg gtgcgcgtgc
      601 gggacttcgc ccaacagcgc cgggaacgcg cgtcctcgca tcccctatat ccgcggcaca
      661 ccgatccggt accggttgcg atcaccaaag tgtggaccgg gccctggggt acaggggaac
      721 ccgtttcggt tcccgagacc tgcatggtgg agatgtactg gcaggcgatg cccggcgaaa
      781 agctggaaga catcgatcgc gagtttcatg catggctgga ttccgttgtg gacagcgcgc
      841 cggatctgtt tccggaacgc ccgcgagcgg aattcccgat acgatggctg ccggggtccg
      901 ctatctcgaa aggagagccg ctggttgcgg aattcgccgc ctgcgccacg gaattgcttg
      961 gagccgaacc ggttatcgcg ggcatcgaag ggccgtgcga catgtacatc tttcaccagc
     1021 agttcggcat ccctgccatc ctctggggag cgaagggagc gaacacgcat gcggccgacg
     1081 aatacgtgga gatcgactcg gtcatcgacg cggcgcgtgt attgctatct ttcatctatc
     1141 gctggtgcgg agccaattga gccatgacgg gccttgagat gcattcggtt gttgcattgc
     1201 tcgaagacct tcccgaagag ggccttgtcc gcggccaggt cggcgctgtg gttgagactt
     1261 gggctccggg ggtatatgaa gtcgagttct gcgatgacaa cggcaggagc tacgccatgg
     1321 tcgcattgaa ggcggatcaa ctaatgcggt tgcatcacgg gcgagttcat cgggcagctt
     1381 aggagtttgt tgaaaaacgt agaaaaccgc ttgctggcgc gcgcggctca gagtcagtaa
     1441 cttacagagc cgcgagcgtc atgaaacagt gactgcaacg cgttttataa gtcgtatcca
     1501 ggcgtaaaac gccgcctctc cgcgcatcga ccttaacttt ccggtacggt cattttggta
     1561 taatcagacc ataagctttg gtgcgggagt tatgtttatc tcccataatt tcgcttcagg
     1621 agtcaagaac ctacagtgag gatctttgtt tgtctactct gcctgctggc gctcgccgca
     1681 ggcttcgcac tgtcgcaaag cacgaatgcg acaatcaccg gcacggttac agacagctcg
     1741 ggagcagtta ttccggccgc caagattacc gtaacgaata tcgacacgaa tctggaagtt
     1801 atcggttcta cgaacgaagt cggcaactac agcctgccgc aacttcgtga gggccgttat
     1861 attctcaccg tcgaagcggc gggcttccag ccctttaccg caagagacgt tgttttgact
     1921 tcgcgggacg tccgccgtat tgacgcggtc ctgcaactcg gccagacggc cacggctatc
     1981 gaagtctcgg ccggagccac tctgattgaa accgaaacgg cgcgcatcag caacacgcag
     2041 accgcagaca tgctgaagag cgtaccgctc aatgcccgct ggctatgggc tttcttcaat
     2101 atcactcccg gatatgtaaa cgcgggtgaa ggctatcgtt tcgccggcgc gcaagcaagc
     2161 caggtgaact gggccatcga cggcaccacg ttttccgaca acgtcggcgg cggatcggct
     2221 atcggaccgc tggcaaacta cgtcgagtcc ttccaggaag tgaaaattga tatggccaac
     2281 aactctgcgg agttcggtac cctgggccag gtcacagtca tttccaaatc cggcgggaac
     2341 agcctgcacg gcagcgctgc cgattacctc cacacgccgt ggttccgcgc acgcaaccca
     2401 ttcgcaaacg cccgcactcc cggtgtctat cacatctatg caggcagcat cggaggacca
     2461 gtctatctgc ccaagattta cgacggcagg aataagacct tcttctatac ggtttatgaa
     2521 ggctccatcg gtggcgatac ggcaacgatg ttgaatccca cggtgccgct cgaatcgtgg
     2581 cgcaaaggcg atttttccag cctgctgccg gggactgcta tttacgatcc caccacggga
     2641 cagccgttcg cgaacaacca gattcccgcc agccggatca accccgtctc ccagaagttg
     2701 caggaccgct actaccctct gccaaacttc ggcgacacca gcagcctgct cgccagtaat
     2761 taccgcgaga tgaagagccg cggctgggat attccgaaat actgggtggt gcgcggcgat
     2821 catcacctga gcgcgaagga cctgatcttc gggcgcttca cctttactcg cggccctgtc
     2881 acgccgtggg agggcaatct gccgacgatc ggacagcgca tccagcgccg cgacacgcgc
     2941 gccgcgtccg cgtcgtggac ccgtaccatc aatgcacaga tggtaaacga agttcgttgg
     3001 ggcatgaacc tcaataacaa cccgctgcaa ggtcccgtga atggtctgga gcaggcgagg
     3061 gaactcggac tgcaaggctt ggcgccggat cttccggatg tcggcggcat gctcaagatc
     3121 cggtggcaga acatcggact gacccctctt cagcagatcg actggacgaa tcctggctac
     3181 cgcaatcaca accaggaatt tcaggagcat ttcagttggt tccgtgggcg tcacaatctg
     3241 cgcatgggct tcgacctgac ccgcgcggag tgggacgagc gagctgcgaa cgatgcgctg
     3301 ttcggtgcgc tgacgttctc ttcgcagtac acctccaacg gcctctccgg ccagggacat
     3361 ccctacgccg acttcctgct cggaattccg actacggccg cgcgtgcgta tcctccggtg
     3421 cggtccgatc gcaatcgctg gaattacgac tttttcatta ccgacgaatg gaaagtcaac
     3481 cagaagctga ctctgaccat cggcgcgcgc tacgaactgc ataagccatg gcgcgagaac
     3541 aatgacttca tctcgctatt cgacatcggt accggcagtg ttgtggttcc cgatggcgcg
     3601 ctctcgaaga tcagcccgct gtatccggcg gactttgctc ctgtcataga agccaaagat
     3661 gcggggctgc cgggccgcac gctgattcgc acggaccgca acaatatcgc gccgcgcatc
     3721 ggcatcgcct atcgcccgtt cagccaggac acagtggtac gcgccggtta tggcatttac
     3781 tacgatccgg ttccgcgcaa ccttacgatg ggcggcatgc cgttcgtcct gcgcgaggag
     3841 ccctatacca acccgctaac gaacccgaca gtcaccctgc cgcgcgtcta tcccgatggc
     3901 gcaaccacaa gagcgactgg cctcagcctg ccggccgctg taaatcccga tctccggttc
     3961 ccttacagcc agcagtggaa tctgaccatc gaacgccagc agtgggacac gggcttccgt
     4021 gtgagctata tcggcacgaa tacccggcag atcgattggg cgtataacat caactcgccc
     4081 gttcccgata gccgtctttt tgtcgaaaag gcgcgcttgt acccgcagta cagcggtatt
     4141 agctactaca ccaatggcgc gaatcaccag tatcatgcgt tgacgaccgc ggttgagcgc
     4201 cgcatgaaga acggcctgat gctacagtcc tcctggactt gggctcggga tatctacgat
     4261 ctgaccagcg gccagatgcc ggagaacccg ttcgaccgcg agcgtgagcg cgccgtgtcg
     4321 cgttccattc ctacccaccg cgtggtgaac agctacgtgt accagcttcc atttggtaaa
     4381 ggacagaaat ggggatcgaa tcttcccaag tacgccgaca tggcagtggg aggatgggcg
     4441 ctcagcggtg ttctcaccct gcaaaccggc cagttcctga cgccggtttg gtcgggcccg
     4501 gatccggttg ggatcgcatt caccggttcg acaacgccag cctcggtgac tcttcggcct
     4561 aatgtactac gcgacccgaa ccttcctgta gatcagcgga cacccggccg ctggtttgat
     4621 gtgagtgcat tcggaccgcc ggccaaaggc agcttcggct ccgcggccaa gggtatcatc
     4681 gaaggtccgg gcatcaattc gtggaatatg ggcttgcaga aagacctgat gttcggcgag
     4741 cgtctaccgc gaatcaacct ggagatcacc gccgtcaata tgtttaaccg cccgaactat
     4801 ggcaacccgg gtatgaacat ttcgcaggcg gccagcgcgg gcgtcatcac cggcctgatc
     4861 ggcgatgact accgggcgcg cgagttccgc gccggtctcc gcgtcgagtg gtaagcaagt
     4921 aaccccagca gttgaagtcg aaggcagccg ggcaaccggc tgccttccta cggattcccg
     4981 accctaatga atccctccgc gggcgaatcg ccgattgtga tgggatgatc gccgtcagag
     5041 agagccggca aaacaaggtt gatctgatac agacccgggc tgtccggaga taggccggcg
     5101 taaaggatat tttccgccgg aacaggtttg ccgtcgattg cgaccggcag cgctgtcgta
     5161 atccgtgcct cgcctgacgc gccggcgccg gcctggaccg ggggattcgc cggacccagc
     5221 gagatgcaat agatcgtgag gatgtctccc ggtttggccg gacggaaggc aagtccggcg
     5281 atgagtccgg gaggaccgat cttcgctcct gtggtcgcat caacggcaac aacagggctg
     5341 cgcccgttcg tcccatgcac ccaatacagg aactccggag ccgttatcct ggcctccacc
     5401 atccatccgg cactcctgat ctcggtagct gtaccgcaat tcgcgactac ctgcacagac
     5461 acgtcggtat ccacgggaat atccggtacc tgaaaattga tctgggccgt tccagcatag
     5521 gtgagaaatg cccgttttcc gcccacctcg acgcacgtgt ttgccagctt cgtggggagt
     5581 actcccgcga cgaaatcact cgattgcact gccgcgtaag ttcccggcgg agcaaaatcc
     5641 gtgccgaaga ccgatgcaag tccgcccggc gataaccggg tcacgggcgc cgcgcttgct
     5701 cccgctccga tcaccgcggc gatgtaggga gctttgggga aggctcccgg cggatagtcc
     5761 ggttggagtc tgcggacccg gtgattgccg gcgtcggcga catagatcgt ttccgttttg
     5821 ggatcaaccg ctatcctctg aggagcccag agtgttgcac tggcggcagg ccctccgtca
     5881 cctgaaaatc cgaaaaaatg agtacccgcg acctgccaac tggttccgtc cggcataagc
     5941 cggcagatcc tgttcgatcc ggagaccagc aggctaccat cagtatcatg ttcgagcccc
     6001 gctagtcccc ataggttcga aaaaatacac tgccgcgcac cgtcgtcgcc gggccggccg
     6061 aacggaatgg tcgggccgat cgttgtcatc acgccatccg gtgtgatccg ccgaaggatt
     6121 gtcgtccctt cgttgatcgt gagcgctccg tgcgtatcgg ctgtaatctg cgaagggtcg
     6181 tagatcggcg acgataccgc cggcgttccc tcaccgcccc ttacgcgctc tccggtcccg
     6241 gcataggtgc ggatcgtgcc atcggtctcg acaacacgaa cgcgattggc ggtgtaatcg
     6301 ctaatgaata gccgcccgcg tgcatccacg gcgagccctc tgaccgattg caaggtcgcg
     6361 ttcgtcgcgg ggccaccgtc gcctgaactg ccgtactcac cggttccggc aacggtctcg
     6421 ataataccgt ccggcctgat gcggcgaacc ttctgcgacg agtcggt
//
LOCUS       DASTMK010000224         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_523310, whole genome shotgun sequence.
ACCESSION   DASTMK010000224 DASTMK010000000
VERSION     DASTMK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523310"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFQ79_09985"
     CDS             complement(<1..334)
                     /locus_tag="VFQ79_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140031.1"
                     /translation="MSFTVFLGNLPTWVTADDIKSWLMAESLVADAVKVIRNPETQES
                     KGFAFIDAPNAEEMQSIIKRFDRAPLEDRLLRANPAQPPRPKGQTGVRPGAPPVQTVD
                     KARRGGKKH"
     gene            complement(527..2116)
                     /gene="serA"
                     /locus_tag="VFQ79_09990"
     CDS             complement(527..2116)
                     /gene="serA"
                     /locus_tag="VFQ79_09990"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEU0140032.1"
                     /translation="MKILIAEPLSPAALDVFKNQRDFDVVVSNPKEYAEHLADADALA
                     VRSAVKVTREVLEKAPKLRVIGRAGVGVDNVDLEAATAAGVLVMNTPGGNAVSVAEHT
                     LALMLALARFIPQADSSTSSGKWEKKRFLGNELRGKTLGVIGLGSIGREVVKRARAFE
                     MRIIANDPYVSSQTAADLNIELLDLPVLYALSDYISLHVALTPETTHMLNTQAFAQMK
                     AGVRIINCARGELIDVDALREALAAKRVAGAGLDVFEPEPPPAGYELFGHDNVITTPH
                     IAGSTEEAQEIVGVRIAQQMVEYLKNGVAINAVNMPALSPEQYKTVRPYILLAERLGA
                     FAAHIATGNPRCVRLVYAGKIASSNTNLVRNAGIAGVLNRSLSQKANLVNAMQLANQR
                     GLDIVEKHETRSEYMDSVRVELETDSGTTAVEGAVLLEKPRLIQVDGIYCEAPLSGHL
                     TFMKNEDVPGVIGHVGSVLGKNGVNIANFSLGRQDSPAHGAPLTAIAVVETDGPLTEP
                     VLSQLREHAAVKLARAVTLPG"
     gene            complement(2136..3311)
                     /locus_tag="VFQ79_09995"
     CDS             complement(2136..3311)
                     /locus_tag="VFQ79_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU0140033.1"
                     /translation="MYIRKQRLLTPGPTPLYPPALQAMMASDLHHRTEDFRKLYRSTL
                     SSLKEIMGTENDVLILVSSGTGAMESSASNFFSKGDKVIVCSAGKFGERWGEIAKAYG
                     LNATVLKAEYGQVVPPEQVRAALDAEPGTRGVFVQASETSTGAAHDVRSMANIVSKTD
                     ALFVVDAITGLGTMPLDIDGWGMDIVIGGSQKAFMIPPGLAFLSISKKAWARSESADL
                     PHYYFDLTKEKKNAASGESSWTPNTSLILALNAALEYIKSIGMDKLVENAQLLARGTR
                     EAVTALGLDLFAPDSPGSSVTAVRAPKDLDSGVIVKEFRNRFNSIIANGQGSMKGKIF
                     RIAHLGYFDFADLFAVISELEIILNANGFPVEFGKGVAAVQKVYADSAVEKQEAVKA"
     gene            3357..3803
                     /locus_tag="VFQ79_10000"
     CDS             3357..3803
                     /locus_tag="VFQ79_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU0140034.1"
                     /translation="MPLLDRVQKDMVQAMKDHDEARLSTLRMMKAALKKQEVDSMKPL
                     DGTSEIQVLNTLMKQRRESADMFRKGGRAELAEKEEAEMRLIETYLPAAASEEDVEAA
                     IAAALSETGVNSLKQMGVVMKAAQAKLAGKRVDGKALSDKVRARLS"
BASE COUNT          786 a         1180 c         1145 g          815 t
ORIGIN      
        1 tgtgcttctt tcctccgcgg cgggccttgt ccaccgtctg cacgggcgga gcgccgggtc
       61 tgacacctgt ctggccttta ggccgcggcg gctgcgcggg atttgcgcga agcaaccggt
      121 cctcgagagg cgcccggtcg aatcgcttga tgatggattg catctcctcc gcattgggcg
      181 cgtcgataaa ggcgaagcct ttggattcct gggtctcggg gttccggatc accttcaccg
      241 catcggcgac aaggctctcc gccatcagcc acgatttaat atcgtcagcc gtaacccagg
      301 taggtaaatt tccgaggaaa acagtaaaac tcattctctg gtgtgaatca ctccgtcttt
      361 gggaattgtt gggacctgaa gggttgggtc catgcgagca ttaccatcgt agcatacgta
      421 aatcgacgcg agcaaacgaa agccacctac tttcagacaa aaaggtcgtt tccaggcaag
      481 gttttcgaag cgcctgaata ctgtgaacgc cggatggtgg attgtgtcaa ccgggcagcg
      541 tcacagcacg cgccagtttc accgcggcat gctcccgtag ctgagaaagc accggttccg
      601 ttaaaggccc gtcggtttct acgacggcta tagccgtaag cggcgcccca tgggctggag
      661 aatcctgccg gccgagcgaa aagttggcga tattgacacc gtttttcccc agcacggagc
      721 cgacatgacc gattacgccg ggaacgtcct cgttcttcat gaaggtcagg tgcccgctca
      781 acggggcttc gcaatagatg ccgtccacct gaatcaaccg gggtttctcg agaagcaccg
      841 ctccttccac cgcggttgtc ccggagtcgg tctccagttc tacgcgcacg gaatccatgt
      901 actcggaacg ggtttcatgc ttttcgacaa tatccagccc ccgctggttt gccagttgca
      961 tcgcattcac caggttcgcc ttctgggata gcgaccggtt cagcacgccc gcgattccgg
     1021 cgtttcgcac caggtttgta ttgctactgg cgattttgcc cgcatatacg agccggacac
     1081 agcgcggatt gccggtggcg atatgcgccg caaaggctcc caggcgttcc gcgagcagga
     1141 tgtacgggcg caccgttttg tactgctccg gcgacagggc aggcatattc accgcgttga
     1201 tggctacccc gttcttcagg tactccacca tctgctgggc gatccgcacg ccgacgatct
     1261 cctgcgcctc ctcggtcgat ccggcgatat gcggagtagt gatcacgtta tcgtggccga
     1321 aaagctcgta gccggccggc ggcggctccg gctcaaagac atccaagccc gctcctgcga
     1381 cccgctttgc cgccaatgct tcgcgcaaag catccacatc aatgagttcg ccgcgtgcgc
     1441 agttgatgat tcgcacgccg gctttcatct gcgcgaacgc ttgtgtgttg agcatatggg
     1501 ttgtctcggg cgtcaacgcc acgtgcagcg agatgtaatc gctgagagca tagagaacgg
     1561 gcaggtcgag caactcgatg ttcaggtccg ccgccgtctg cgaactgaca taaggatcgt
     1621 tggcgatgat acgcatctcg aacgcgcgcg cgcgcttcac cacttcccgg ccaatactgc
     1681 cgagcccgat cactcccagc gtttttcctc gcagctcgtt accgagaaag cgcttctttt
     1741 cccatttgcc gcttgaggtg gaggaatccg cctgcgggat gaaacgggcc agcgcgagca
     1801 tcaacgccag ggtgtgctcc gctaccgaga ccgcgttgcc ccctggcgta ttcatcacca
     1861 ggacacccgc cgccgtcgcc gcttccaggt cgacattgtc gactccgacg cccgcgcggc
     1921 cgatgacgcg caacttggga gctttttcca gcacctcccg cgtaaccttg accgcgctgc
     1981 gaactgcgag ggcatcggca tccgcaagat gctctgcata ctccttcgga ttggagacga
     2041 ctacgtcgaa atcccgctgg ttcttaaaga cgtccagcgc cgccggggag aggggttccg
     2101 ctatcagaat tttcatcgtg tagagtggct cttcctcagg ccttcacggc ttcctgcttt
     2161 tctacggccg agtccgcata cactttctgg accgcggcca cacccttgcc gaattcgacg
     2221 ggaaatccgt tggcgttcag gatgatctcg agttcggaaa tgacggcaaa caggtcggcg
     2281 aaatcgaagt accccaggtg ggcaatacgg aaaatcttgc ccttcatcga tccctggcca
     2341 ttggcgatga tcgagttgaa acggttgcgg aattccttca cgatcacgcc ggaatccaaa
     2401 tctttcggcg cacgcacggc ggtcacggac gagccgggag agtccggcgc aaacagatcg
     2461 agcccgagcg ccgtcacggc ctcccgtgtg ccgcgcgcca gcaactgcgc gttctccacc
     2521 agcttgtcca ttccaatgga cttgatgtat tcgagggcgg cgttcaatgc gagaatcagg
     2581 ctggtattcg gcgtccaact cgattcgccg ctcgcggcat tcttcttctc tttggtgaga
     2641 tcgaaatagt aatgcggaag gtcggcgctc tcggagcgcg cccacgcctt cttgctgatc
     2701 gacagaaacg ccagccccgg cggaatcatg aatgctttct gcgatccgcc gattacgatg
     2761 tccatgcccc atccgtcgat gtcgagcggc atagtgccca atccggtaat ggcatccact
     2821 acaaagaggg cgtctgtctt cgaaacgata ttggccatgg agcgcacgtc gtgcgccgct
     2881 ccagtcgacg tttcggaagc ctgcacgaat acgccgcgcg tgccgggttc ggcatcgagc
     2941 gccgcccgca cctgctccgg aggaaccacc tggccgtact cggctttgag gaccgtcgcg
     3001 ttcaggccgt aggccttcgc gatctcgccc caacgctcgc cgaattttcc ggccgagcaa
     3061 acaatcacct tgtccccttt ggaaaagaag ttggaggcgg acgactccat tgcaccggtt
     3121 cccgacgaga ccaggatgag gacgtcgttc tccgttccca ttatttcttt gaggctggag
     3181 agtgttgagc ggtacagctt gcggaagtcc tccgtgcgat gatgcagatc gctggccatc
     3241 atcgcttgca gggcgggtgg atagagagga gtggggcccg gggtcagtag cctttgcttg
     3301 cgtatataca tgtcggttat gaggatgtga ttagatattc tagaatatca aacgtcatgc
     3361 ccttactcga tcgagtccag aaggacatgg tccaggcgat gaaggaccat gacgaagccc
     3421 gtctcagcac tctgcggatg atgaaagccg ccttgaagaa gcaggaggtg gattccatga
     3481 agccgctcga tggaacctcc gagatccagg tgctgaacac cttgatgaaa cagcgccgcg
     3541 aatccgcgga catgttccgc aagggcggaa gggccgagct ggccgaaaag gaagaggcgg
     3601 agatgcggct gattgagacg tacctgcccg ccgccgcgtc cgaagaggat gtggaggcgg
     3661 ccatcgccgc cgcgctatcg gagaccggcg tgaattcact caagcagatg ggtgtggtaa
     3721 tgaaggccgc ccaggccaaa ctggccggca aacgcgtcga cggcaaggca ttgagcgaca
     3781 aagtgcgcgc gcggctatcc tgaggaatcc ggcctttcag tctgtcatgt ctttccaact
     3841 tcgaggactc gccgaatgaa cgagaagcaa tacaccccct acgtgccgca gaacgtcagt
     3901 atgcgggagt tcacggcacg ggccct
//
LOCUS       DASTMK010000225         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_266836, whole genome shotgun sequence.
ACCESSION   DASTMK010000225 DASTMK010000000
VERSION     DASTMK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266836"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFQ79_10005"
     CDS             <1..657
                     /locus_tag="VFQ79_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140035.1"
                     /translation="ALTDVDLRSYSSGAQQNQYQRYLERADDGNIRRQQLRFSYLYDL
                     PFGRGRRFGSGLPRAVDFFAGGWQVAGITTMMTGARLSPTYSNADPANTNQFGGRPDR
                     IGDGNFDSGEMSDSIRARQPILDRNAFVLPASGRGFYGNSARYLLTGPGNMTWNMGVH
                     KNWNIKERAQMQFRWELFNAFNRVNFSNPTTNIQSGSFGLVTSAGSGRSMLFGLRLDY
                     "
     gene            complement(723..1865)
                     /locus_tag="VFQ79_10010"
     CDS             complement(723..1865)
                     /locus_tag="VFQ79_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140036.1"
                     /translation="MSASPSRRNFVANSGAAAAASLGFLIVKPESVSGSQANSALTVG
                     LIGAGRRGTAISGIFAKNEFARIAAICDIYDDQLEAASKKFAGAKTFKDLNDLLASDV
                     DAVYIATPPYLHPPHFEAAVKARKHIFVEKPAGVDAAGCRRVIEAAKKADKSKRISFD
                     YQQRYGKEYNQAYDLVKSGELGKIAMVRGSWLGGGLPVRTGHPSDQEKMRNWLFYREY
                     SGDIIVEQNCHNLDVVNWFMGKHPVKANGYGGRKVRTDIGNIADHLAANFLFADDVVF
                     SYSANQIAQGGYRDVGEVFFCEKGVVTTSRTGIEIYREGSRKPEVFPTKYDITQDAVN
                     QFVEGARTGKIENAAFWAAESTLTAIMAREAIYAGKEMTWDQISRA"
     gene            complement(1986..>5085)
                     /locus_tag="VFQ79_10015"
     CDS             complement(1986..>5085)
                     /locus_tag="VFQ79_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140037.1"
                     /translation="DVKLELGQVTEQITVEASAAALQTDKSDVRSEITSQAASNLPLN
                     AYRNFQNLINLVPGATPANFQNSVGSTPARALTTNVNGTARNNNNARVDGATNVYIWL
                     PHHMVYVPPVESISTVNITTGSFDAEQGMAGGAAITVATKSGTNEFHGSAFEYHDNQR
                     LRTRNFFLPASSGKAKSIFNIFGGTLGGPIVKDKLFFFGSFEGTLERAGISRTTDSVP
                     TSAIRNGIFTGLPVTLYDPTTGDADGNGRQPFADNVIPANRINPISRRIQELAPQPNL
                     AGTSQGTLNNYFSSGTEKLNRYNYDVKVNWNPTSTLMIWGKYSRMDAAVDSKFAFGEL
                     GGPGLSRAGAPEVSDVGVNIPTFGYTKTFSPTFLLDGTISITRMDNFGVYPGYGENTG
                     SEVWGIPNTNDPVVTGAGGADRLKDACPSLANGGCYTGMPRIIMGFTDWGATAGWQPY
                     FYNERSLTYTTNATKMSGSHEIRFGFDMVRYQMDQWQPEVSGGPRGSITFSGDVTGAR
                     GYTANYLNQYATYLLGLTTSMQKAVQLFQYTNREWQFGGFLRDRWQVSRKLTLNMGIR
                     YEYYPLIYRKDRGIERWDPATNLVYMGGVGDIPRNVGITSSKKLFAPRLGFAYRLTEN
                     TVVRSGYGITYDPLPFSRPLRGMYPATIGATFVTTTPYNWIDTLDKGIPAISLPDTSS
                     GIIELPPTIDMGPRSAWGGEIHRGYIQSWNFTLERKLPAEISTSIGYVGTQTVHQMMD
                     RDINAAPPGGGPAGRPLAATQNRRIAALMWDGSASGNYHSLQVAMNRQFSQGLLLKGA
                     YTWSKAINWIDDDGWTGAPTFNWEPMMYRNRATAGYNRAHMFTLGSVYELPFGPGKKY
                     VVSGPLSWLVRGWQTNGTFAAYTGTPFTVSAAATELNAPGNSQTADQVNSGRVQTIGE
                     IGANKSWFDPLAFRQPTGVRFGTSGRNSIFGPGMWNLDLSLFRNFNVTERVKIEFKAE
                     GFNITNTPKFSNPGANVASMVLNTDGTVRALNNYSSITSTLPNMGAPSERQLRFGLRL
                     SF"
BASE COUNT          956 a         1506 c         1499 g         1124 t
ORIGIN      
        1 gcgctgaccg acgtggacct gcgctcctac tcttcgggcg cccagcaaaa ccaatatcag
       61 cgatacctgg agcgggcgga cgacggcaac attcgccgcc agcagcttcg cttcagctac
      121 ctgtatgacc tcccgttcgg ccgtggccgg cgcttcggaa gcgggctgcc aagggcggtg
      181 gatttcttcg ccggaggatg gcaagtcgcg ggtattacga cgatgatgac cggggcacgg
      241 ttgagcccca cctatagcaa cgccgatccg gcgaacacga accagttcgg agggcggccg
      301 gaccgcatcg gcgacggcaa tttcgattcc ggggagatga gcgattcgat tcgcgcgcgg
      361 cagccgatcc tcgaccggaa cgcgttcgtg cttccggcct ccgggcgcgg cttttacgga
      421 aactccgccc gctatcttct gaccggtccg ggcaatatga cctggaacat gggcgttcac
      481 aagaactgga acataaaaga gcgcgcacag atgcaattcc ggtgggagtt gttcaacgcg
      541 tttaaccggg ttaacttttc gaatccgacc accaacatcc agagtggcag tttcgggtta
      601 gtaacgtcgg cgggttccgg gcgatcgatg ctgttcggcc tgcgcctcga ttattgatac
      661 acgctccctc gcgcgcggct cagaaccgag ccgcgcgcgc cagcaagcgg tatcggagat
      721 acttacgctc tcgatatctg gtcccaggtc atctctttgc ccgcgtaaat cgcttcgcgg
      781 gccatgatcg cggtgagtgt gctttcggcg gcccaaaagg cggcgttctc gatctttcca
      841 gtccgtgccc cttctacgaa ctggttgacc gcgtcttgcg taatgtcata cttcgtcggg
      901 aagacttccg gtttccggga accttcgcga tagatctcga tgcccgttcg cgaggtggtt
      961 accacgccct tctcgcaaaa gaacacctcg ccgacatcgc ggtatccgcc ttgcgcaatc
     1021 tggttggcgc tgtagctgaa caccacatcg tcggcaaaca ggaagttggc cgcgaggtgg
     1081 tccgcgatat tgccgatatc ggtacgcacc ttccggccgc cgtaaccgtt cgccttcacg
     1141 ggatgttttc ccatgaacca gttcacaacg tccaggttat ggcaattctg ttcgacgatg
     1201 atgtctcccg aatactcacg atagaagagc cagttccgca tcttttcctg gtcgctggga
     1261 tgtccggttc gaaccggcag gccaccgccc agccaggagc ctcgtaccat cgcgatcttt
     1321 cccagttcgc cagatttaac caggtcgtag gcttggttgt actcctttcc gtagcgctgc
     1381 tggtaatcga aggaaatccg tttggactta tccgccttct tggcggcctc gataacgcgc
     1441 cggcagccgg cggcatcgac gccggccggc ttctccacaa agatatgttt gcgagccttg
     1501 accgccgcct caaaatgcgg tggatgcaga tagggcgggg tagcgatgta caccgcgtcc
     1561 acgtcactcg ccagcagatc gttcaggtcc ttgaaagtct tcgcaccagc gaatttcttc
     1621 gacgcagctt ccagttgatc gtcgtagatg tcgcagatgg ccgcgattct cgcaaactcg
     1681 ttttttgcaa agatcccgct gatagcggtg cctcgccggc ccgctccgat caggcccaca
     1741 gtcagcgccg agttggcctg ggagccggag acggattccg gtttcacgat caggaagccg
     1801 agagacgccg ccgcggcggc gccggagttc gcaacgaaat ttcttctgga aggactagcg
     1861 ctcattaatt tctccaaagc gagggaaacg aaaccggatc tatacggatg aatatcgctc
     1921 gcccaccatt atacggggat ctcatgcggc agatgagatc cccgctaact tcgtcgttat
     1981 cgcggtcaga aactgagccg caagccgaat ctcaactggc gctcggacgg agcgcccatg
     2041 ttcggcaatg tgctggtgat cgaggaatag ttgttcaatg cccgcaccgt gccgtcggtg
     2101 ttcagcacca tcgacgcgac gttggcgccc gggttggaga acttgggcgt attcgtaatg
     2161 ttaaagcctt ccgccttgaa ttcgatcttc acgcgctccg tcacgttgaa attgcggaaa
     2221 aggctcagat cgagattcca catgcccggg ccgaagatcg aattgcgccc gctggttcca
     2281 aagcgcacac cggttggttg ccggaatgcc agcggatcga accacgactt gttcgcaccg
     2341 atctcgccga tggtttgcac ccttccggag ttaacctggt ccgccgtctg gctattgccg
     2401 ggagcgttca actcagtagc cgccgcggag accgtgaacg gtgttccggt gtatgcggcg
     2461 aatgtgccgt tggtctgcca tcctcgcacc agccaggaaa gcggcccgct gaccacgtat
     2521 ttcttgccgg ggccgaacgg cagctcatag accgagccca aggtgaacat atgggcgcga
     2581 ttgtagcccg cggtggcgcg gttgcgatac atcatcggtt cccaattgaa agtaggcgcg
     2641 cccgtccatc cgtcgtcgtc aatccaattg atcgccttcg accaggtata ggcgcctttc
     2701 aaaagcaggc cttgactgaa ctgccggttc attgccacct gaaggctgtg ataattgccg
     2761 cttgccgagc cgtcccacat cagcgcggca atccggcgat tctgcgttgc ggccagcggg
     2821 cgtcctgccg gcccgccacc gggaggcgcg gcgttgatat cgcgatccat catctgatgt
     2881 acggtctgcg tgcctacgta cccgatgctg gttgagatct cggcaggcag ctttcgctcc
     2941 agagtgaaat tccaggactg gatatagccg cggtgaattt cgccgcccca tgcgctgcgg
     3001 ggacccatgt caatcgtcgg cggcagttcg attatgccgc tgctggtgtc gggaagcgat
     3061 atcgcgggaa tccctttatc gagcgtgtcg atccagttat agggtgtcgt ggtcacgaag
     3121 gtcgcgccga tcgtcgccgg atacattccc cgcaggggcc gggagaaagg cagggggtcg
     3181 tacgtgattc catatccgct gcgtactacc gtgttttccg tcagccggta agcgaacccc
     3241 aggcgcggag cgaacagttt tttgctgctc gtaatgccga cattccgcgg gatatcgccc
     3301 acaccgccca tgtataccag gttggtggcg ggatcccacc gctcgatgcc gcgatctttc
     3361 cggtagatca gcgggtaata ctcatagcgg atgcccatat tcaaagtgag cttgcgagac
     3421 acctgccagc ggtcgcggag gaacccgccg aattgccact cccggttcgt gtactggaac
     3481 aactgcacag ccttctgcat agaggtggtg agccccagaa gataggtggc gtactggttc
     3541 aggtagttgg ccgtatatcc gcgcgctccc gttacgtcgc cgctgaaagt gatgctcccg
     3601 cgcggtccgc cgctcacttc gggctgccac tgatccatct ggtatcgaac catgtcgaac
     3661 ccgaatcgga tttcgtgaga tccgctcatt ttcgttgcgt tcgtcgtata ggtgagcgat
     3721 cgttcgttat agaagtacgg ctgccagccg gcggtagctc cccagtcggt gaaacccatt
     3781 atgattcttg gcatgccggt gtagcagccg ccattcgcca ggctcgggca ggcgtctttc
     3841 aggcggtcgg ctcctcccgc tccagtcaca acggggtcgt tggtgttcgg aataccccaa
     3901 acctcgctgc cggtgttctc tccgtaaccg gggtagactc cgaaattatc cattcgcgtg
     3961 atggagatcg tgccatccag caggaaggtc ggggaaaagg ttttggtgta gccgaaggtg
     4021 gggatattga caccgacgtc ggatacttcc ggcgctccgg cgcggctcag ccccggcccg
     4081 ccaagctctc caaaggcgaa cttcgaatcc accgccgcat ccatgcggct gtacttgccc
     4141 cagatcatca gagtgctggt cgggttccag ttcactttca catcgtagtt gtaacggttg
     4201 agcttctccg tgccggaact gaagtagttg ttgagagtac cttgtgaggt cccggccagg
     4261 ttaggctggg gcgcgagttc ctgtattctc ctgctgatgg gattgatgcg gttcgccggg
     4321 atcacgttat cggcgaaggg ttgccgtccg ttgccgtccg catcacccgt cgtcgggtcg
     4381 taaagagtaa ccggcagtcc cgtaaagatg ccgttccgga tcgcgctggt tggaaccgag
     4441 tcggtggttc tgctgatgcc ggcgcgttcc agggttccct caaagctgcc gaaaaagaac
     4501 agcttgtcct tcacgatcgg cccgcccagc gtgccgccga aaatattgaa gatcgacttc
     4561 gctttgccgc tgctcgccgg caggaagaag ttgcgcgttc tcagccgctg attatcgtga
     4621 tactcaaaag ccgagccgtg aaattcattg gtgcccgact tggtggccac cgtaatcgcc
     4681 gctccacctg ccataccctg ctcggcatcg aaggagcccg tcgtaatgtt cacggtactg
     4741 atggactcga cgggaggcac ataaaccatg tggtgcggca gccagatata gacgttggtt
     4801 gcgccatcca ctctggcgtt attgttgttg cgcgccgtgc cgttgacatt ggtggtcagt
     4861 gcgcgcgccg gtgtggatcc cacgctgttc tgaaagttgg cgggcgtggc ccccggaacc
     4921 aggttgatca ggttttgaaa attacggtag gcgttcagcg gaagattgct cgccgcctgg
     4981 ctcgtaattt ccgaccgcac atccgacttg tcggtctgca acgcagcggc gctggcttcc
     5041 actgtaatct gttcggttac ctgtccgagt tcgagtttga cgtcc
//
LOCUS       DASTMK010000226        19099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_170909, whole genome shotgun sequence.
ACCESSION   DASTMK010000226 DASTMK010000000
VERSION     DASTMK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19099
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170909"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..798
                     /locus_tag="VFQ79_10020"
     CDS             238..798
                     /locus_tag="VFQ79_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140038.1"
                     /translation="MRSLNLRTLLALLCAFLAEVAVFRILNAATSAHPVGMGARISGM
                     FVVLIAVPAVYGIVRHGWRDELARTLALLAIAGIVGMSFHWVLAARLVAAAAILGILF
                     LLFRTSGMKWFAALVITLAISLLGFFLSTLALSAFVRCDPQNRWLCDLAGFTMHVLTA
                     LIACAGYVYGRKTALRIAAQRTPYFA"
     gene            complement(774..1457)
                     /locus_tag="VFQ79_10025"
     CDS             complement(774..1457)
                     /locus_tag="VFQ79_10025"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEU0140039.1"
                     /translation="MPVNSSPVLPAELELFQHIESSLYTAVLSDALDELGIRDRAMRE
                     HLRPLDPDSVFAGRARTILCMDVHYIAKEPYRKEIEALDSLLPGEVAVVATGNSKRNA
                     PWGELLSTAAMARGARGAVVDGLVRDVKKILALRFPVFAAGVKPVDSRGRGEVVDYNV
                     PVECGGVLVSPGDLIVADFDGVISISSNAVEEVVRLATGKATRENSSRAELMKGAYLR
                     DVYAKYGVL"
     gene            complement(1475..1909)
                     /locus_tag="VFQ79_10030"
     CDS             complement(1475..1909)
                     /locus_tag="VFQ79_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140040.1"
                     /translation="MKGDAGVWLRYMLIAFVANGLGPFGLKILSERGLAGYQSQYLLY
                     WYLGGVIFALLAFSRGLGRVPAREVLIGAAMGVSSLSGQSFTGLALARDVPGHIVFPI
                     TTGGSLFLVAAAGILVFRERVGLYGLAGILLGIASLVLLSIP"
     gene            complement(1906..2415)
                     /locus_tag="VFQ79_10035"
     CDS             complement(1906..2415)
                     /locus_tag="VFQ79_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140041.1"
                     /translation="MHWGGSRENQRAVPLHHPMGTGHLYMLLALLSFSTLGVFHKLAD
                     VKKCRPSAITALLYLWSLAFAFLLLTTVQRATPAAPATVVSIGLPFGVSAAVAILAFQ
                     AGVRHGNIATSWLAINLSSGIPTVGSILIYDEPVSLTKAIALSLIPVAMLLLWKDKKS
                     SAAAPGELS"
     gene            complement(2422..5619)
                     /locus_tag="VFQ79_10040"
     CDS             complement(2422..5619)
                     /locus_tag="VFQ79_10040"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0140042.1"
                     /translation="MRKALSLIPVLLLAAATLFGQAGTEGSILGTVQDASGAVIPGAQ
                     ATAVNLNTGLKRTVVTNETGSFEIFALPIGQYSVAVTAKGFKTWNLARVEITVGARNR
                     ITPVLEVGEVSEQISIEATAELLQTEQSSVQTVVQMHQIRELPLSVRNPVVLVNLVAG
                     MRYIGQGGPERGSTVQGFGTRDNQTQFALDGLNANAAMDEGGMAIPNVDTIAEFNVQT
                     NSFSAENGRNPIQVQIATKSGTNEFHGALWEFLQNDNLNARNTFAVSIPKLRRNQFGA
                     AVGGPIFRNRTFFFASFEGTPIRGQSIYNSATVNPAMLNGDFSSLPRQIKDPTTGEAF
                     PGNVIPASRISSASKFFFPYVLLPNSPGDRFRANAATKNDTWEGTGRIDHQITDNQRI
                     YGRWVVVDNTSSSPGYTPEIVSDTTTRQHSVGVNYTYALRPNMLLNASAGYLNSDNRF
                     GSPITGIDNLVEQAGIRGIPTAGREDFVGLPNVGITGYTGFNAPWGVPGRLWSNVKNA
                     KAGLNVIHGFHTLNFGYEFQDRAVFGRHGSHSPRGSFDFNGQYTGDGFADYLLGLTSG
                     SRRNYPLETFGLTNAPYSGAWIQDFWKLTPNLTLSAGLRYERWHERKLRNGNGATFDP
                     TIGKVIAGVDDDGQMNLTAQPVARFLAAATEGLWIPATEANVPYGLSEGNGNWSPRVG
                     ITWRPSMRSDFVVRAGYGLFYNSFTGNRAASSIVGPPYWAWEALTFSALSLQQWETAW
                     PEDPQAFIQPSIGEAPAWNLHQAKTHEWNISVQTALPFKSALTVSYVGTRIHDQVAMR
                     AYNEVPPGAYPNLQAAKPYPAFGAINVLENLGRSWYNGLQLKWERRFAEGLSFMGAYS
                     FAKDISDTTPAGEFDTFVPFAPEGYLRGRSGYDRTHLFTANAVWQMPFGRGRRWGADL
                     PRVADLIAGGWELSGIYSFRSGAPLSISVPGATLGNGWGTRAVLNGDPKVSNPDASRW
                     FNTAVFSAPPQYQWGSSGIGILDGPASQILDAALMKNFHITEGRYFQFRLEAYNAFNR
                     VNLGNPGTTLGTATFGQILSAGGARTVQIGLKFIF"
     gene            complement(5709..8495)
                     /locus_tag="VFQ79_10045"
     CDS             complement(5709..8495)
                     /locus_tag="VFQ79_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140043.1"
                     /translation="MRACIISILAACSLSAASQSERKVRMSWGHDAPAATDYYIRLVP
                     GPGVRAGAATGVSLETGEVSKDGVWQTRAGAQDIDGIDFIIGYDVEAPRRIDNLHITW
                     SDLISTSDADTSRRLMQDPAFRLNSRKLTVQMDPQGTRGFSVTVDQLLEQKAIFVPSL
                     HVYLTTGDRPVPFEEHMQTLKSWMGRRILDEVQREPEATYDYYASRWEDMGSPSYVHP
                     QQPRPGHIVALTWDSAIPKFGIDRGAGVWNDYGNPDRFQFWYSFGDLTSGIASSWKKQ
                     SLHDGLPVITTVFEKEGVRYEVEQFAYPLDGPPRERRGDIGMVLMQKVRLIELEGKQH
                     GVQFTISQRRQFPAFFNSSIEGEREGHRLLFRLAGRREVLFTIDGPFDDAQWSGVRDY
                     QRDQKRLDVFLTTSLEPRGTREFIVKLPSPMVEPADAEKLIALDYTQSREQTIGFWSN
                     WVERGAQFRVPEKAVNDLFRAALWHALRLPRRHGGSGPDVRIDLPYSNFAYSQTGTPW
                     PVNQAVYVDYMLYDLRGYHSVSAEELLAQYRNNQEANGHVAGYANWVVYTPSMLYSVA
                     QHYLLSGDRETFEKLLPASMKALDWCLEELRKAARASGHVMGLVRGPLNDGTGDGVWA
                     FNQAYMYAGLEMFGRALERAGHARTAECRRAAQTLREAIQRGFQAAAMNSPLVPLRDR
                     TWVAYVPCEARTFRRLIDDWYATDVDTGAVHMIRLKAVDAGGPLADSLLHDHEDNLYY
                     KGWGIANEPVYNQQATAYLLRDDPKAVIRAFYSYMASAFSHSTWEPVEHRWTHGQYFG
                     PPSTDGAWFDLYRNMLIHERDDGALVIGLATPRAWLKPGNKIEIERAPTYYGPVSARI
                     QSGAAPGSISAEVEMPTRATPDALLVRLRHPEEKRMRSVTVNGQPWKDFDPDREWVRI
                     SKPSAKQYRIQASY"
     gene            complement(8495..10624)
                     /locus_tag="VFQ79_10050"
     CDS             complement(8495..10624)
                     /locus_tag="VFQ79_10050"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU0140044.1"
                     /translation="MIIAILALLLVVSPRAAADPLAEKVTIYRDTYGVPHIVGETEEA
                     TFFGYGYAQAQDHLERMMVQYLDAQGRRTEVQGFQALGDGYLHFIPYEYRWDGDYLQR
                     LLQTKKGVVDGRDKIPPDVYRILDAFARGVNFYIRENRARIPAWIEEITAEDVEALER
                     SQYFRFYSIHDALSKLPGGSTYSFPHLGSNQWAVARGKSANGRVMHVEHTHMPWANRF
                     QNYEAHLMTPGKLNASGISWFGSPFFLDGFNDRITWSATYNNPNIADVYEEKLKPDDP
                     AKYEYDGRWRAMRVEEETFRIRGPKGVETVTLPLYYTHHGPVVKVDRTRHRAWSVKLP
                     NFENVTYSAALYGLMKARNLAEFKAVVARQWMPRWNLLYSDAKSIYWVHNGTVPLRPA
                     GFDWSKPVPGWTSATEWTGYVPFEQYPQVLDPPSGFLQNCNNPPWFVTAGSELKSPAP
                     YYLHSPDAALNTRGERLLKVLGENRKFSLADMIDLAFDTYIVAAEIFIPLLERTDPQD
                     EQARRAVALLKEWDRRSSVDSTAYTIAYYFGKAYQDLFAGRFSRFNSPARTKIDIASA
                     EEQKMAAEALREALNRLEKRFQSIEVSWGRINITRRGGDFPMDGTGLYGVLHPDAGIE
                     QEDGRILCNDGWGHVMVTVEGDPKEIWTLLPYGQSEDPSSPHYNDQTILHSRRQLKRF
                     WFTPAEILNNTKSVWGKRERLESIREQ"
     gene            10751..13477
                     /locus_tag="VFQ79_10055"
     CDS             10751..13477
                     /locus_tag="VFQ79_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU0140045.1"
                     /translation="MRLLLAAAVVSAVCYSATRPRIDLNGTWEFRTDPQSIGDTQEWH
                     SPGVPFDRRLQVPGTWQAQGVGEPAGIVRNHYEGQAWYRRQVAIPASWKGKTVHVRVG
                     GALRRVALYVNGHRIGEHDGISAPFEFDVTRAIRPGSENTIAFRIVNPGATINEGPRT
                     QPGDEPTGMVNYIASWGGLYGNVELEASDPLRIAEVAIVPSVERKTAAFRVSVAGADS
                     ASGVRVEVNVGGATGAAALRGGEAEATVAIPQARLWSPEDPHLYTATVRLVQGANERD
                     RIEERFGMREVTTRGNVLLLNGKPLYLRGYGDDMIEVLTGAPPASKQVYLERLRMARS
                     FGFNAVRFHSATPLPAFFEAADEVGIFVMAELPVAYTQYFLPHRDFLKREMEGVLRAH
                     RNSPSFLSLAFGNEFNLGWLETDAERKEFQASVEEFYRLAKSIDPQRLILSNDGLVLR
                     PTDMFSLYTGAPEDAPTVRHEFGNYYCSLPDISLIPKFTGVIEPTWLKEKKAWVEKGG
                     LAAKYAEYVRNSQRLQHIGRKYQIERVRRSPEVTGYHYWLIVDYPGGTGEGDSWEEGW
                     FDYFWQPKGIQPAEGRELNSPILLLLSAGVNERTLWNDTEKTMDVFVSNYGNDPVQGG
                     VLRWRLLDGTREIASASVADVHSELGTVTKAGRITLPPTGGEEARKLQLVAEIAAGTN
                     TYTNRWNFWTFPRKALLDRSPVPVASSVRWASVRRLYPFITDAAAGATPDSLLIVSRL
                     DAEALRHIESGGRVWLMADREQFGRPGDATFFPASGGALGSVLQDHAALAGMPHEGMF
                     DLQFYNLMEGAWSLPLDNWPQELVPIAGGIRTTSSFLSKRKNLSRTGYIFEAKVGKGS
                     LLVTALAIREHLDEAYPEAVFLFDRLLRYASGSGFRPVVEVSAEFLRKQMAR"
     gene            complement(13478..14227)
                     /locus_tag="VFQ79_10060"
     CDS             complement(13478..14227)
                     /locus_tag="VFQ79_10060"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0140046.1"
                     /translation="MDRLKNKIAIVTGAAHGIGRAIAQLFAEEGAWVLIADVDEQAGE
                     AAAAWIREQGGGVVFQRTDVSSATDAARAVEIAGAQSGRIDILCNNAAYLGEFHGVLE
                     ATDDEWNRCIQVALLGTQRCTREVLPYMIRQKQGSIVNIASIQAIAGCPASVAYTATK
                     AALLGFALSAAYDYGPSNIRVNSICPGPIQTRISPKPGEPHYDWQCAQTMLGRVGYPE
                     EVAHAALFLASDEASYITGAVLPVDGGWTSK"
     gene            14316..15164
                     /locus_tag="VFQ79_10065"
     CDS             14316..15164
                     /locus_tag="VFQ79_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0140047.1"
                     /translation="MTAAKTPSVPALERGLAILELVAHSKSGLTFSQIARHLEFPKSS
                     IHCLLLTFEREGYLRRSETTGRYLCGSKLIRIANLAMDGIVLRERALPILRDLMESTG
                     MTVHMAILERGEATLIAKLERGRVHRVATWIGKRIDVHCTSLGKCLIADLPDEELRRL
                     IEERGLLRHNENTIASLHKLRQELAKTRSLGYAVDDEEEEIGIRCIGAPIAGPAGNVL
                     AAISISGTTEQIQDSNSAALVNLVKQTAREISRQLAEFRDSRSFEEPAALARSAGQDS
                     SGGTLP"
     gene            complement(15134..16663)
                     /locus_tag="VFQ79_10070"
     CDS             complement(15134..16663)
                     /locus_tag="VFQ79_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140048.1"
                     /translation="MNRRHFLELGTMSAALASGSSAATSQAGAQPSGSAGYAERDITP
                     KIGMEQPGGYSKVYHKSLHDPCKVRAAVFQDGGTRVALVGIDALIIPRSVVVGARKEI
                     EQRCGIPGNAVMIGASHSHSSGPVGMVLPGEYDHASPLAKKLAYEHSSAADAGYLEQV
                     RAEIVNAVYQADKSRRASRTAFGTGTEEKAAYNRRFRMKNGLTFTHPGKGNPDIVEAA
                     GPIDPEVAAIGTFDEQGKLIGCVVNYACHATTSPGGISANWIYYMENAIRGAFNPDAV
                     VVFLQGFCGDVTQVDNLSPYANPSGEEWARFVGGRVGAEAVKVLLSAEAGSLGPVAVR
                     TKLLQIPRRKPNPARLKKCIEMVQKDPKEAGMTDWVFAKEIVMLDALIAKEPRVDVEI
                     QAIQVGPAVFVAAPGEMFCQLGLDIKAGSKFPITCPVELANGCVGYIPTEEALGKHGG
                     GYETRLTAYSNLIPSAGTQMVNAGVELTKQLTPGKFPEPAKAPPFKEPWSYGNVPPEL
                     S"
     gene            complement(16708..17850)
                     /locus_tag="VFQ79_10075"
     CDS             complement(16708..17850)
                     /locus_tag="VFQ79_10075"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0140049.1"
                     /translation="MQPPTSFRISRRAVLGTPLASVALGAQQESGFTSLFDGKTLDQW
                     SVQDGSETAFYVRDGEIVIHSGSGYPAWLRSSRQYENFDFRCDFHIQGWSDSGIFIHA
                     PEHGRNTWAGMQIKLFHQQEKEPTTNAMGSIFPVVAPSKVNVRPGWNSIRILMDWPTL
                     QVWTNDEMVQDVNVETTADLCYRLREGYLGLSSLSYPLRFRNLRVRELPSKQKWQILY
                     NKPEDLGTNWWLSEGEPNFQALGGILRADGLGHIATKEKYRDLELQLYIRGTRSHNGG
                     LLFRSEGKGLKGKRYEIQLHDVPDSHFPTGSLYYYKRSRYPNIEDEKWFLAQLFVKDR
                     NVVVRIDGENVLEYDNLTDLEPGYVELQAHRKGYWLEFKDVRIRRI"
     gene            complement(17857..>19099)
                     /locus_tag="VFQ79_10080"
     CDS             complement(17857..>19099)
                     /locus_tag="VFQ79_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0140050.1"
                     /translation="QEVLAGGVSSGLRRSMRPHPLYFTGGEGARVRDVDGNTYLDYGL
                     AWGPLILGHCPPEVTKQVGEQLQRGLTYGAQHDLEFEVAEALTSIIPCADLVCFANSG
                     TEIVQVALRLARAATGRPKYLKFEGHYHGWDDSVLVSYHPAAEQISASGDNPIPVGRG
                     QRSAQEAIVAQWNDREQVERIFAEHSGEIAAVICEPLLCNTFCIPAEPGFLEFLRAIT
                     ERHGCLLIFDEVITGFRLDLKGAQGVYGVVPDLATYAKAVGAGTALSVLAGRRQYMER
                     IANGEVVHAGTLNGNPISLAAAAAALEVLSRNDGAVYGEMRRLSGKLRSGIEAALRAK
                     GLPVVTSGEGPVFHVSFMEEPARNYRDTLRADQSAYTQLAIALLDEGVLVLQDGRWYV
                     SAAHDDAAIDETLSALDRALS"
BASE COUNT         3618 a         5818 c         5788 g         3875 t
ORIGIN      
        1 cgcgcgaaat ccgagcacca cagctttatc gacggcctgc atcgcttccc aatgcttctc
       61 caggtcgacg ctgatgtctt tgtaggcgtc gcccgccgtc tggcgcgcat cggcaagaaa
      121 tgattctcca atgtggcacg gcgtttccca aacgtgggta tggacgtcaa taatcatcaa
      181 taatcctcac ttgcttatgc gcgcggctcc gcaacatact tgatctgggt ccgcataatg
      241 cgctcactta atctccgcac cttgctcgcg ctcctttgcg catttctggc ggaggtggcc
      301 gtctttcgta ttctaaatgc cgccacttcg gctcatccgg ttggaatggg cgcacggatc
      361 tccgggatgt tcgtagtctt gatcgccgtc cccgcggtgt atggcatcgt ccgccatgga
      421 tggcgggacg agttggcccg caccctggcg ctgctggcaa tcgccggtat agtgggcatg
      481 agttttcatt gggtgctcgc cgcccggctg gttgccgccg ccgctatcct gggaatcctg
      541 ttcctccttt tccggaccag cggaatgaaa tggtttgctg cgcttgtgat cacgctggcc
      601 atatcgttgc tgggattttt tctcagcacc ctggcgctgt cggcgtttgt gcgatgcgat
      661 ccgcagaacc gctggctctg cgacctggct ggatttacca tgcacgttct gacggcgttg
      721 attgcctgcg ccggatatgt ctacggccgg aagacggctc tccgcattgc cgctcaaagg
      781 acaccgtact tcgcatagac gtcgcgcagg taggctccct tcatcaattc cgcgcggctg
      841 ctgttctcgc gcgtagcttt gccggttgcc agccggacca cctcttcgac ggcgttcgaa
      901 gagatggaga tgacgccgtc gaaatcggcc acaatcaggt cgccgggtga aaccagcacg
      961 ccgccgcatt ccaccgggac gttgtagtcc accacttcac cgcggcctcg cgaatcgacg
     1021 ggtttcacac cggccgcgaa taccgggaag cgcaacgcga gaatcttctt cacgtcgcgc
     1081 accaggccgt ctacgaccgc gccgcgggcg ccgcgcgcca tggccgccgt cgaaagcaat
     1141 tcgccccatg gcgcattgcg tttcgaattg ccggttgcca ccaccgccac ctcgcccggc
     1201 aacaggctgt cgagcgcttc aatctccttt cgatagggct ctttcgcgat gtagtgcacg
     1261 tccatacaga ggatcgtgcg ggcgcgtccc gcgaaaacag aatcgggatc gagcgggcgc
     1321 aggtgctccc gcatcgcgcg atcgcgtatt cccaactcgt ccaacgcatc cgataacacg
     1381 gctgtgtata ggctgctctc aatgtgttgg aacagctcca gctcggctgg caagaccgga
     1441 ctgctattaa ccggcaagat gacgcctcct taaattacgg gatgctcagc agcaccaggg
     1501 aggcgatgcc gagcaggatc cccgctaaac catataaacc cacacgctcc cggaaaacca
     1561 gtatgccggc ggcggcaacc aggaacagag agccacccgt cgtgatcgga aaaacgatat
     1621 gcccgggaac gtcgcgcgcc agagccaggc ccgtgaaact ttgaccgcta aggctcgaaa
     1681 cacccatcgc ggcgccaatc aaaacctctc gggcgggcac tcttcccagt cctcgtgaaa
     1741 atgccagcag agcgaagatc accccgccca gataccagta cagcagatac tgcgactgat
     1801 agcctgccag cccgcgctca ctcagaatct tcagcccgaa ggggccgagt ccgttcgcga
     1861 cgaacgcgat cagcatgtag cggagccata cgcccgcgtc tcctttcacg acagctctcc
     1921 gggcgccgcc gcgctggact ttttgtcttt ccacagaaga agcatcgcca ccgggatgag
     1981 cgaaagcgcg atcgctttcg tcaggctgac cggctcgtcg tagatcagaa tcgagccgac
     2041 ggtcggaatg cccgaggaaa gattgatcgc aagccagctt gtggcgatat tgccgtggcg
     2101 gactccggcc tgaaacgcga ggatcgcgac ggcagccgaa actccgaacg gcaagccgat
     2161 ggagacgacc gttgcggggg ctgccggtgt ggcgcgctgc acggtagtca gcagcaggaa
     2221 cgcgaaggcg agcgaccaga gatacagcag cgcggtgatt gcgctcgggc ggcatttctt
     2281 gacatccgcc agcttgtgaa atacgcccag cgtgctgaaa ctcagcaacg cgaggagcat
     2341 gtaaagatgt ccggttccca tgggatggtg gagagggaca gcccgctggt tttcgcgcga
     2401 accgccccaa tgcacatcat ttcagaagat gaactttaaa ccaatctgca ccgtacgggc
     2461 gccgccggcg ctcagaatct ggccgaacgt ggcggtgccg agcgtggtgc cggggttgcc
     2521 aagattcaca cggttgaacg cgttataagc ctccaggcga aattggaaat aacgtccctc
     2581 cgtgatgtgg aagttcttca tcagcgcggc gtcgaggatc tggctggcag ggccatccag
     2641 gatgccaata cccgaactgc cccactgata ctgcggcggg gcggagaaga cagcggtgtt
     2701 aaaccaccgg ctggcatcgg ggttcgagac cttcggatcg ccgttgagaa ccgccctggt
     2761 tccccagccg ttgcccagcg tcgcacccgg cacggagatg ctgagcggtg cgccggaccg
     2821 gaagctgtag atgccgctca actcccagcc gccggcaatg agatccgcca cgcgcggcag
     2881 gtccgctccc catcgccggc ccctgccgaa cggcatctgc cagacggcgt tcgctgtaaa
     2941 caggtgcgtg cgatcgtagc cggaacgccc gcgcaggtat ccttccggag caaacggcac
     3001 gaaggtatcg aactcgccgg cgggtgtcgt atcgctgatg tccttagcga acgaataggc
     3061 gcccatgaag gagaggccct ctgcgaagcg gcgttcccat ttcaactgca agccgttgta
     3121 ccaggagcgg ccgaggtttt cgagcacgtt gatcgctccg aatgccggat agggctttgc
     3181 cgcctgaagg ttcggatagg cgcccggggg cacttcgttg taagcgcgca tggccacctg
     3241 gtcgtgaatc cttgtaccca cgtaggaaac ggtgagcgcg gatttgaacg gcagcgccgt
     3301 ttggaccgag atgttccact cgtgcgtctt tgcctggtgg aggttccacg ccggagcttc
     3361 tccgatggag ggctgtatga acgcctgcgg atcttccggc catgccgttt cccactgctg
     3421 gagagaaagc gcgctgaagg tcagcgcctc ccacgcccaa tacggcggac ccacaatgga
     3481 cgatgctgcg cgattgccag tgaagctgtt gtagaacagc ccgtagcccg cgcgcacgac
     3541 gaaatcgctc cgcatgctgg gccgccacgt gatgcccacg cgtggagacc agttgccgtt
     3601 gccctccgaa agcccatagg ggacgttcgc ttcggttgcg ggaatccaca ggccctccgt
     3661 ggccgccgcc aggaaccgcg ccacgggttg ggcggtgaga ttcatctggc cgtcgtcgtc
     3721 cacaccggcg atgactttgc cgatggtggg gtcgaaggtt gccccgttgc cgttacgcag
     3781 cttccgctcg tgccaccgtt cataccgcag acccgccgac agtgtcaggt tcggcgtcag
     3841 tttccaaaaa tcctgaatcc aggcgccgga ataaggagcg ttcgtcaggc cgaaagtctc
     3901 cagcggatag ttgcggcggc ttccggacgt caggccgaga agataatcgg cgaatccatc
     3961 gcccgtgtac tggccgttga aatcgaagct gccgcgagga gaatgcgagc cgtggcggcc
     4021 gaacaccgcg cggtcctgga attcgtatcc aaagttcaac gtgtgaaagc cgtggatgac
     4081 gttcagtcct gccttggcgt ttttcacgtt gctccagaga cggccgggaa ctccccaggg
     4141 cgcgttgaaa ccggtgtagc cggtgatgcc gacgtttggc agcccgacga aatcctcgcg
     4201 tcctgctgtg ggaatcccgc gaatgcccgc ctgttccacc aggttgtcga tgccggtgat
     4261 gggcgagccg aagcggttgt cggagttgag ataccccgcg ctcgcattga gcagcatgtt
     4321 cggccgcagc gcgtaggtgt agttcacacc cacgctgtgc tggcgcgtgg tggtatcgga
     4381 gacaatctcc ggagtgtagc cgggcgagct cgatgtattg tcgacgacga cccaacggcc
     4441 atagatgcgt tggttgtccg tgatctggtg atcgatgcgt cccgtgcctt cccaggtgtc
     4501 gttcttggtt gcggcatttg cccggaaccg gtcgccgggg gagttgggca gcagaacata
     4561 agggaagaag aacttcgatg cggaggagat ccggctcgct ggaattacgt tccccggaaa
     4621 agcctcgccg gtggtgggat ctttgatctg cctcggcagt gaggagaagt cgccattcag
     4681 catggccggg ttgaccgtcg ccgagttata gatggattgc ccgcgaatcg gcgtgccttc
     4741 gaagcttgcg aaaaagaagg tccggttgcg gaaaatgggg ccgcccaccg ccgcgccgaa
     4801 ctggttccgg cgaagcttcg ggatgctcac ggcgaatgta tttcgggcat tcaggttgtc
     4861 gttctgcaaa aactcccaca gtgctccgtg aaattcgttg gtgccggact tggtggcgat
     4921 ctgtacctgt atgggattgc gtccgttctc cgcgctgaag ctgttcgtct gcacgttgaa
     4981 ctcggcaatg gtatccacgt tgggaatggc catgccgcct tcgtccatcg cggcgttggc
     5041 attcagcccg tccaaagcga actgtgtctg attgtcgcgc gtgccgaatc cttgtacggt
     5101 agagccgcgc tccggcccac cctgaccgat gtagcgcata ccggccacca ggttcaccag
     5161 cactacggga ttacggacac tcaatggcag ctcgcggatc tggtgcattt gtacgacggt
     5221 ctgaaccgaa ctctgctctg tctgaagcaa ctccgcggtc gcttcaatcg agatctgctc
     5281 gcttacctcg cccacctcca ataccggtgt gatgcggttg cgcgcgccga cggtaatttc
     5341 aactcttgcg aggttccagg tcttgaaccc cttggccgtc acggcgacgg aatactggcc
     5401 aatgggcagc gcaaaaatct cgaacgagcc ggtttcgttt gtgacaaccg ttctcttcaa
     5461 acctgtgttc aaattaacgg ctgttgcttg tgccccggga atcacagcac ccgaggcatc
     5521 ctgaactgta cccaagatcg atccttcggt tccagcctgt ccgaacagtg tggctgcggc
     5581 aagcaacaga accggtataa gcgagagggc ttttctcata agcatctcct gtgcgcttaa
     5641 ttctggatcg ataggctgat tgaatcaatg caaaacccgg tttggattcc agagtattgg
     5701 aaacaacttc agtagctcgc ctgaatgcgg tattgcttcg cagagggttt cgagatccgg
     5761 acccattcgc gatcggggtc gaaatctttc cagggttgtc cattcacggt gacggaccgc
     5821 atgcgctttt cctcgggatg ccgcaggcgc acgagcagtg catccggtgt ggcgcgagta
     5881 ggcatctcga cttccgccga aatgctgccc ggcgcggcac cgctttggat acgcgcggac
     5941 acagggccgt aataagtggg cgctcgctcg atctcgattt tgtttcccgg cttcagccat
     6001 gcgcgcggcg tggccagtcc gatgaccagc gcgccgtcgt cgcgttcatg gatcagcatg
     6061 ttgcggtata agtcgaacca ggcgccgtcc gtggaaggag gcccaaaata ctggccgtgc
     6121 gtccagcggt gctcgaccgg ctcccacgtg gagtggctga atgcactcgc catgtagctg
     6181 tagaacgcgc ggatgacagc tttcgggtcg tcgcgcaaca ggtacgccgt tgcctgctgg
     6241 ttataaaccg gttcattcgc gatgccccag cctttgtagt agaggttgtc ctcgtgatcg
     6301 tgcagcaggg aatccgccag tggtccgccg gcgtcgacgg ccttcaggcg aatcatgtgt
     6361 acggcgccgg tgtccacatc ggtggcgtac cagtcgtcga tgaggcggcg gaacgttctt
     6421 gcttcgcacg gcacgtaggc aacccaggtc cggtcgcgca acggaaccag cggagaattc
     6481 atggctgccg cctgaaagcc gcgttggatg gcttcacgaa gggtctgtgc cgcacgccgg
     6541 cactccgcgg tgcgtgcatg cccggcgcgt tcgagggcgc ggccgaacat ctccaggccc
     6601 gcgtacatgt aggcctggtt gaacgcccac acgccgtccc ccgttccgtc gttcaagggc
     6661 cctcttacaa gccccatcac gtgcccggaa gctcgcgcgg cttttcgaag ttcttccagg
     6721 caccagtcga gcgctttcat gctcgcgggc agcagctttt cgaatgtttc gcgatcgccg
     6781 gaaagcaggt agtgctgtgc gacggagtac agcatggagg gtgtgtagac gacccagttg
     6841 gcgtatccgg cgacgtgacc attcgcctcc tggttgttgc ggtactgtgc cagcaattcc
     6901 tcggccgaaa ccgaatgata gccgcgcaga tcgtagagca tgtaatcgac gtaaaccgcc
     6961 tggttcacgg gccagggcgt accggtctgg ctgtaagcga agttcgaata gggaaggtcg
     7021 atgcgcacat ccggtccgct gccgccatgc cggcgaggga ggcgtagcgc atgccagagc
     7081 gccgcgcgga atagatcgtt taccgctttc tccggcacgc gaaactgcgc gccgcgctcc
     7141 acccagttcg accagaaccc gatcgtctgc tcgcgggatt gcgtgtagtc gagtgcgatg
     7201 agcttttcgg cgtcggcggg ctccaccatg ggcgaaggca gtttgacgat gaactcgcgg
     7261 gtgccgcgcg gctccaggct ggtggtcaga aaaacgtcga gacgcttctg atcacgctga
     7321 taatccctga cgccgctcca ctgcgcgtca tcgaaggggc cgtctattgt gaagaggact
     7381 tcgcgccggc cggcaaggcg gaacaacagg cgatggcctt cccgctctcc ttcgatgctg
     7441 gagttgaaaa atgccggaaa ctgccggcgc tggctgatag tgaattggac gccgtgctgc
     7501 tttccttcca gttcgataag ccggaccttc tgcatcagga ccatgccgat atcgccgcgc
     7561 cgctcccgcg gcggcccgtc gagcggatag gcgaactgct ccacttcgta ccgcacgccc
     7621 tccttttcga atacggtcgt gatcaccggc aatccatcgt gcaagctttg cttcttccag
     7681 gaggacgcga taccggaggt caggtctccg aagctgtacc agaactgaaa gcggtcgggg
     7741 ttgccatagt cgttccacac tccggcgccc cggtcgatgc caaacttcgg aatcgcgcta
     7801 tcccaggtga gcgctacgat gtggccgggg cgcggctgtt gcggatggac gtacgaagga
     7861 ctgcccatgt cttcccagcg cgaggcgtag tagtcgtaag tggcctccgg ctcgcgttgc
     7921 acctcgtcca gaatgcgccg gcccatccac gatttgagcg tctgcatgtg ctcttcaaac
     7981 ggtaccgggc ggtcaccggt ggtgagatac acgtggagcg acggcacaaa gatggctttc
     8041 tgctccagca gttgatcgac cgtgacgctg aatccgcgcg tgccctgcgg gtccatctgc
     8101 acggtcaatt tgcgcgaatt caggcggaag gcggggtcct gcatcagacg gcggctcgta
     8161 tcggcgtcgc tcgtgctgat gaggtccgac caggtgatgt gcaggttgtc gattctacgc
     8221 ggagcctcca catcgtagcc gattatgaaa tcgatgccgt cgatatcctg cgcgccggcg
     8281 cgtgtctgcc agacgccgtc tttcgacact tcgccggtct ccagcgatac tcccgtcgcg
     8341 gcaccggccc ggactccggg tcccggcaca agccggatgt agtagtccgt cgcggccggc
     8401 gcgtcgtgcc cccaggacat gcgaaccttc cgttcgcttt gggacgcggc gctcaagctg
     8461 cacgccgcga ggatcgatat tatgcaggct ctcattattg ctcccgtatg ctctccagcc
     8521 gttcgcgttt cccccacacc gatttcgtgt tgttcagaat ttcggccgga gtgaaccaga
     8581 accgtttcaa ttggcggcgg ctatgcagga tggtctgatc gttatagtgc ggcgaagacg
     8641 ggtcttcgga ttggccatac ggcaacaggg tccagatctc tttcgggtcg ccttcgacgg
     8701 tcaccatcac gtggccccag ccgtcgttgc acaggatgcg cccatcctcc tgctcgatgc
     8761 ccgcatccgg gtgcagcacg ccgtagagcc ccgttccgtc cataggaaaa tcgccgccgc
     8821 ggcgggttat attgatcctg ccccaggaca cttcgatgct ttgaaatcgc ttctccaacc
     8881 ggttcaaggc ttcgcgcagc gcctccgcgg ccatcttctg ttcttcggcc gaagcgatat
     8941 caatcttggt acgcgcgggt gaattgaatc gcgagaaccg gccggcgaac agatcctggt
     9001 aggcctttcc gaaatagtag gcgattgtgt aagccgtgga atccacgctc gagcgccggt
     9061 cccactcctt caacagcgcg acagcgcggc gcgcctgttc atcctgcgga tccgtacgct
     9121 ccagcagcgg aatgaatatc tctgcagcga caatgtaggt atcgaaagcg agatcgatca
     9181 tgtccgcgag gctgaacttt cgattctcac cgagcacctt caacaggcgc tctcctcgcg
     9241 tgttgagggc cgcatcaggc gagtggaggt aataaggcgc tgggctcttc agttccgaac
     9301 cggcggtgac gaaccacggc ggattgttgc agttttgcag aaaccccgat ggtgggtcga
     9361 gcacctgtgg atactgttcg aacggaacat aaccggtcca ttccgttgcg ctcgtccagc
     9421 ctggaaccgg cttgctccaa tcgaagccgg ccggccgcag gggaaccgtg ccgttgtgca
     9481 cccagtagat gctcttcgca tcactgtaaa gcaggttcca gcgcggcatc cattgccgcg
     9541 ccaccacggc cttgaattcc gccagattgc gcgccttcat caatccatag agagcggcgg
     9601 agtaagtgac gttttcgaag tttgggagtt tcaccgacca ggcacgatgc cgcgtacgat
     9661 ccactttcac gacagggccg tgatgcgtgt aataaagggg tagcgtgacc gtctccacgc
     9721 cctttggtcc gcgaattcgg aacgtttcct cttccacgcg catcgcgcgc cagcggccgt
     9781 cgtactcgta tttcgcaggg tcgtccggct tcagtttttc ttcgtacacg tcggcgatgt
     9841 tgggattgtt gtacgtggcc gaccacgtga tccggtcgtt gaatccgtcc agaaaaaacg
     9901 ggctgccgaa ccaactgatg ccgctggcgt tgagtttgcc gggcgtcatc aggtgcgctt
     9961 cgtaattttg aaaacggttt gcccacggca tgtgcgtatg ctcgacatgc atgacgcgtc
    10021 cattcgccga tttcccgcgc gcaactgccc actggttcga acccaggtgc gggaaagagt
    10081 acgtgctgcc gcccggcaat ttcgaaagcg cgtcgtggat gctgtagaag cggaagtact
    10141 ggcttcgctc cagcgcctcg acgtcttccg cggtgatctc ttcaatccag gccggaatgc
    10201 gcgcgcggtt ctcgcggatg taaaagttca cgcccctggc gaaggcgtcc aggatgcgat
    10261 aaacatcggg tgggatcttg tcgcgtccgt ctacgactcc ctttttcgtt tgcagcaatc
    10321 gctgaaggta gtcgccgtcc cagcgatatt cgtacggaat gaagtgaaga tacccgtccc
    10381 ccagcgcttg aaaaccctga acctccgtgc gtcgcccctg cgcgtcgagg tactgcacca
    10441 tcatccgctc gaggtgatct tgcgcctgcg cgtagccata gccgaagaac gttgcctctt
    10501 ccgtttcgcc gacgatgtgc ggcacaccgt acgtgtcgcg ataaattgtg accttttcgg
    10561 cgagagggtc agcagccgct cgcggagaaa caaccagcag gagcgcaaga atcgcgatga
    10621 tcatccgtta cgcgattata cgtgcggacg gtaccggcca ctcgcattcc atgacggcaa
    10681 tttccagcta attggagagt aatgcgcgcg gcaaccggtt cattgccgcg gctggtgtta
    10741 cgttctagga gtgagactac tgctagccgc cgctgttgtt tcagcagtgt gctattccgc
    10801 tacccggccg cgcatcgacc tgaatggcac ctgggagttc cgcactgacc cgcagtccat
    10861 tggcgatacg caggagtggc actcgcctgg tgttccgttc gatcgccgtt tgcaggttcc
    10921 aggcacgtgg caggcgcaag gcgtgggcga accggcgggc atcgtgcgaa accactatga
    10981 agggcaggcg tggtatcgcc ggcaggttgc cattccggca tcgtggaaag ggaaaacggt
    11041 gcatgtgcgc gtaggcgggg cgctgcgccg ggtcgcgctt tacgtcaatg gccaccggat
    11101 cggcgagcac gatggcatca gcgcgccgtt cgagtttgat gtgacgcgag ccattcggcc
    11161 gggctcggag aatacgatcg cattccgcat tgtgaatccg ggcgcgacca tcaacgaagg
    11221 cccgcgcacg cagcccggtg acgagcccac tggaatggtc aactacatcg caagctgggg
    11281 cggtttgtat ggaaacgtcg agctggaagc gagcgacccg ctgcgaattg ccgaagtggc
    11341 gattgttcct tccgtcgagc gaaagacagc cgccttccgc gtgtccgtcg ccggagcgga
    11401 ctctgcttcc ggtgtgcgtg tcgaggtgaa cgtgggcggt gccacaggcg ctgccgcgtt
    11461 gcgcggcggc gaagcggaag cgacggtcgc gattccacag gcgcgtttat ggtcgccgga
    11521 ggatcctcat ctctatactg caaccgttcg attggtgcag ggcgcaaacg agcgcgaccg
    11581 tatcgaagaa cgttttggaa tgcgcgaagt cactactcgc ggcaacgtgc tgctgctcaa
    11641 tggcaagccg ctttacctgc ggggctatgg cgacgacatg atcgaagttt tgacgggcgc
    11701 tccacccgct tccaaacagg tgtatctgga acgcctgcgg atggctcgca gcttcgggtt
    11761 caatgccgtg cggttccatt cggccacgcc tcttccggcg ttcttcgaag cggcggatga
    11821 agtgggcatc ttcgtcatgg cagaattgcc tgtcgcgtat acgcagtatt ttctgccgca
    11881 cagggatttt ctgaagcgcg aaatggaagg ggtgctgcgc gcacatcgca atagcccgtc
    11941 atttctctcg ctggcgttcg gcaacgaatt caacctgggc tggctcgaga ccgatgccga
    12001 acgcaaggag ttccaggcca gcgtggagga gttctatcgc ctggcgaaat ccatcgaccc
    12061 gcaacgcctc atactgtcga acgacggcct ggtgctgcgg cccacggaca tgttcagcct
    12121 gtacaccggc gctcccgaag acgctcccac agtgcgccac gaattcggaa actactactg
    12181 ctctctgccg gacatttcgc tgatcccgaa gttcaccggc gtgatcgagc ccacctggct
    12241 caaggagaag aaggcctggg tggaaaaggg cggcctcgcg gcgaagtacg cggagtacgt
    12301 cagaaattcg cagcggctcc agcacatcgg gcgcaagtac cagatcgagc gtgtgcgccg
    12361 cagccccgaa gtaaccggat accattactg gctgattgtc gattaccctg gcggaacggg
    12421 cgagggcgac tcgtgggaag agggatggtt cgattacttc tggcagccga aaggcattca
    12481 gccggcggaa ggcagggagc ttaacagccc catcctgctg cttctcagcg ccggggtgaa
    12541 cgagcgcacc ctgtggaatg acacggaaaa aacgatggac gtgttcgtgt ccaattacgg
    12601 aaacgacccg gttcagggag gcgttctccg atggcggctg ctcgacggca cgcgcgaaat
    12661 cgcgtccgct tccgtcgcgg atgttcattc ggaattgggt acggtgacga aagccggccg
    12721 gatcacgctt ccgccaacgg gaggggagga agcccgcaaa ctgcaactgg tggctgagat
    12781 tgcggccgga accaacacct acacgaaccg gtggaacttt tggacgtttc cgcggaaggc
    12841 gctgctcgac cggagccccg tgccggttgc ttcttccgtg cggtgggcat cggtgcgccg
    12901 gctgtatccc ttcatcacgg acgctgcggc gggtgcgacg cccgattcgt tgctgatcgt
    12961 gtccaggctc gacgcggagg cgctccggca catcgaatcc ggtggacgcg tctggctgat
    13021 ggcggaccgt gaacaattcg gacgccccgg cgatgcgaca ttcttcccgg cttccggcgg
    13081 cgcgttgggc agtgttttac aggaccacgc ggcccttgcg ggcatgcctc acgaaggcat
    13141 gtttgatctc cagttctaca acctgatgga aggtgcatgg tctttgccgc tcgacaattg
    13201 gccgcaggag ttggtgccca ttgcaggcgg tattcgcacc acttccagct tcctgagcaa
    13261 gagaaagaat ctgagccgca caggctatat tttcgaagcg aaggtcggaa aaggaagcct
    13321 tctggtgaca gcgctcgcga tccgcgaaca tctggatgag gcctatccgg aggccgtgtt
    13381 tctttttgac cgcctgttgc gttacgcctc cggctccggg tttcggccgg tagtagaggt
    13441 tagcgcggaa ttcctccgga agcagatggc gcgctgatta tttggaggtc cagccgccat
    13501 ccaccggcag gacggcgccc gtgatatacg aagcttcgtc cgaagcgaga aacagtgcgg
    13561 cgtgggccac ttcttccggg tagcccacac ggcctaacat cgtctgcgcg cactgccagt
    13621 cataatgcgg ctcgccgggt ttcggggaga tgcgcgtctg aatcggcccc gggcagatcg
    13681 agtttacgcg aatgttgctc gggccatagt cataggcggc gctcagcgcg aagcccagaa
    13741 gcgcggcctt cgtcgccgta taagcaacgg acgccgggca cccggcgatc gcctgaatcg
    13801 aggcgatgtt cacgatggaa ccctgtttct gccggatcat atacggcaac acttcgcgcg
    13861 tacaccgctg cgtacccagg agggccacct gaatacaccg gttccattca tcgtccgtag
    13921 cctccagcac tccgtggaat tcgccgagat aggccgcgtt gttgcagagg atgtcaatgc
    13981 ggccggattg cgcaccggca atctccaccg ctcgcgcggc atcggtggcc gacgacacat
    14041 cggttcgttg aaacaccacg ccgcctccct gttcgcgaat ccatgccgcg gcagcttccc
    14101 cggcctgttc gtccacatcc gcgatcaaca cccaggctcc ttcttccgcg aagagctgtg
    14161 cgatcgcccg cccgatgccg tgcgccgctc ccgtaacgat tgcgattttg tttttgagcc
    14221 tgtccatttg acatccattc ttttggtcca ggtaccggca tgtcaaaggc ggttctgtta
    14281 gaattgcctg cacagtccaa cctattggaa aacatatgac ggctgcgaag acaccttccg
    14341 tgccggctct ggagcgtggc ctcgccattc tggagctggt cgcacattcg aaaagcggcc
    14401 ttacgttctc gcagatcgcg cggcacctgg agtttccgaa gagctcgatt cactgcctgc
    14461 tgctcacctt cgagcgcgag ggatacctcc gccggagcga gacgacaggc cggtatctgt
    14521 gcggttcaaa gctgatccgt atcgccaacc tcgcgatgga cggaattgtg ctgcgagagc
    14581 gggcgctccc gattttgcgc gacctgatgg agagcacggg catgaccgtc cacatggcga
    14641 tactcgagcg cggcgaagct acgctgatcg cgaagctgga gcgcgggcgc gtgcaccgcg
    14701 tcgccacgtg gatcggcaag cgcatcgacg tgcattgcac cagtctcggc aagtgcctga
    14761 tcgcggacct gccggatgaa gaacttcgcc gcctgatcga agagcgcggg ttgctgcggc
    14821 ataacgagaa cacgatcgcg tcgctccaca agctgcggca ggagctggcg aaaacgcgca
    14881 gcctgggtta cgccgtggac gacgaagagg aggagatcgg catacgatgc atcggcgctc
    14941 ccatcgccgg acctgcgggc aacgtgctcg ccgccatcag tatctcgggc acgacggaac
    15001 agattcagga ttccaacagc gcggctttgg taaatctcgt gaagcagacc gcgcgcgaga
    15061 tctcacgcca actggcggaa ttccgcgata gccggagctt cgaggaaccg gctgccctcg
    15121 cccggtccgc gggtcaggac agctccggtg gaacgttgcc gtaagaccag ggctctttga
    15181 agggcggcgc tttcgcgggc tcgggaaatt ttccgggggt aagttgtttt gtcagctcta
    15241 caccggcgtt caccatttgc gtgcctgcac tgggaatcag attgctataa gcggtcagcc
    15301 gcgtttcgta accgcccccg tgtttgccga gagcctcttc cgtgggaatg tagccgacac
    15361 agccgttcgc cagctccacc gggcaggtga tggggaattt gcttccagcc ttgatgtcca
    15421 gccccaactg gcagaacatt tcgccgggcg cggcaacaaa tacggccggg cccacttgaa
    15481 tggcctggat ctccacgtcc acccgcggct ctttggcaat gagcgcgtcg agcatcacga
    15541 tctctttcgc aaacacccag tccgtcatgc cggcttcttt cggatctttc tgcaccattt
    15601 cgatgcactt cttcagacga gcgggattcg gcttgcgccg cggaatctgg agcaacttcg
    15661 tgcgaacggc gaccggacct agggatccgg cttcggcgct cagcagtact ttcaccgctt
    15721 cggcaccgac gcggccgcca acgaaccgcg cccactcttc gccggacggg ttcgcgtaag
    15781 ggctcaggtt atccacctgc gtcacgtcgc cgcagaaacc ttgcaggaac accactacgg
    15841 catcaggatt aaacgcaccg cgaatcgcgt tctccatgta gtaaatccag ttcgccgaaa
    15901 taccgcccgg gctggtggtc gcatggcacg cgtaattcac gacgcagccg atgagcttcc
    15961 cttgttcatc gaaggtgccg atagctgcca cctcggggtc gatggggccg gcggcttcta
    16021 cgatatcggg attccccttg ccggggtgag tgaaagtgag gccgtttttc attcggaagc
    16081 ggcggttata ggcggccttt tcttccgtcc ccgttccgaa cgcggtgcgg ctcgcgcggc
    16141 gcgatttgtc cgcctggtaa acggcgttga cgatctcggc ccgcacctgt tcgaggtagc
    16201 cggcgtcggc agcggaggag tgttcataag cgagcttttt cgccagggga gaagcgtgat
    16261 cgtactctcc cggcagcacc attcctacgg ggcccgagga atgcgagtgc gacgcgccga
    16321 tcatgaccgc attgcctgga atgccgcaac gctgttcgat ctccttgcgt gcgccaacaa
    16381 caacagaccg ggggatgatg agcgcatcga tgccgaccag tgccacacgc gtgccgccat
    16441 cctggaacac cgccgcgcgc accttacagg ggtcatgcag cgacttgtgg taaaccttgc
    16501 tgtagccacc cggctgctcc atgccgatct ttggcgtgat gtcgcgctcc gcgtatcccg
    16561 cgctgccgga cggctgcgcg ccggcttgag aggttgccgc gctggaaccc gaagccaatg
    16621 cggcggacat cgtacccaat tccagaaaat gcctgcggtt cataactgtg ccccttctgc
    16681 caaataccgc tccgtcgccg tcgcggctca gatccttctg attctcacgt ctttgaactc
    16741 cagccagtag cctttccggt gcgcctgcaa ctccacatat ccaggctcca gatcggtgag
    16801 attgtcgtat tcgagaacgt tttctccatc aatgcgcacc accacgttcc ggtcttttac
    16861 gaacaactgt gccaaaaacc atttctcgtc ttcaatgttt ggatagcggg agcgcttgta
    16921 atagtacaga gagccggtcg ggaaatggga atcgggcaca tcgtgcaact ggatttcata
    16981 ccgcttgcct ttcagcccct taccctcgga acggaacagc aatcctccgt tgtgcgaccg
    17041 cgtcccacgg atatagagct gcaattcgag atcgcgatac ttctctttcg tggcgatgtg
    17101 gccgagtccg tccgcccgca ggatgccgcc cagcgcctgg aagttcggtt ccccttccga
    17161 tagccaccag ttcgttccca ggtcttcggg cttgttgtac aggatctgcc acttttgttt
    17221 ggagggcagt tcccggacgc gcagattgcg gaatcgcaac ggataggaaa ggctcgacag
    17281 gcccaggtat ccctccctca accggtagca aagatcggcg gtggtctcga cgttgacatc
    17341 ctgcaccatt tcgtcgttgg tccagacctg aagcgtgggc cagtccatca gaatccggat
    17401 ggaattccag cccggccgca cattcacctt ggacggagcc actacgggaa agatcgagcc
    17461 catcgcattg gtagtgggct ccttctcctg ctgatggaag agcttgatct gcatgcccgc
    17521 ccaggtattg cggccatgct ccggcgcatg aataaagatg ccggaatcgg accagccctg
    17581 gatgtgaaag tcgcagcgaa agtcgaaatt ttcgtattgc cggctggacc ggagccaggc
    17641 gggatatcca gagccggaat ggatcacgat ctctccgtcc cggacataga acgcggtttc
    17701 cgaaccgtcc tgaacactcc actggtccag ggtcttgccg tcaaagaggc tggtgaatcc
    17761 gctttcctgc tgtgcaccaa gcgccacgga cgccaaggga gttcccaata cggcgcggcg
    17821 gctgattcga aaggatgtag gaggctgcat aaacggtcag gacagcgcgc ggtcgagcgc
    17881 gctcaacgtt tcgtcaatag cggcgtcatc atgggcggcc gatacatacc accgtccatc
    17941 ctgcaatacc agaactcctt catcgagcag cgcgatcgcc agttgcgtgt atgcgctctg
    18001 gtccgcccgc agcgtgtcgc ggtaattacg tgcgggttcc tccatgaagg acacgtggaa
    18061 caccgggcct tcgccggagg ttacgacggg gagccctttc gcgcggagcg cggcttcgat
    18121 gcccgagcgc agcttgccgg aaagacgtcg catttcgcca tacactgctc catcgttgcg
    18181 cgacagcact tcgagcgcgg cagccgccgc cgcgagcgaa atcggatttc cgttcagagt
    18241 gcccgcatgc acaacctcgc cattggcaat ccgttccatg tactgccgcc tgccggccag
    18301 cacgctgagc gctgttcccg cgccaacggc tttggcatac gtcgccaggt ccggcacgac
    18361 gccatacacg ccctgcgcac ccttcagatc caggcgaaag ccagtgatca cttcatcgaa
    18421 gatcaacagg cagccgtgcc gttcggtgat ggcgcgcaga aattcgagga atccgggttc
    18481 cgcgggaatg cagaacgtat tgcataacag cggttcacag ataacagccg caatctcacc
    18541 gctgtgctcg gcgaagatgc gttccacctg ctcccggtcg ttccactggg caacgatcgc
    18601 ctcctgtgca cttcgctggc cgcgccctac aggaatcggg ttgtctccgg aagcggaaat
    18661 ctgctcggct gccggatggt agcttaccag cacgctatcg tcccagccgt ggtaatggcc
    18721 ctcgaatttg aggtatttcg gccggccggt ggcggcgcgt gcaaggcgca gcgccacctg
    18781 tacgatttcc gtgccgctgt tggcgaagca caccaggtcc gcgcagggaa tgatcgacgt
    18841 gagtgcttcg gcgacctcga actcgaggtc atgctgagcg ccgtaggtca gtccccgctg
    18901 caattgttct cccacctgtt tcgtgacctc tggtggacaa tggccgagaa tcagcggtcc
    18961 ccaagccagg ccgtaatcga ggtatgtgtt tccgtccaca tcgcggacgc gcgcgccttc
    19021 gccccccgtg aaatacagcg ggtgcgggcg catacttcgc cgcaatccgc tcgaaacgcc
    19081 tccggccagt acctcctgt
//
LOCUS       DASTMK010000227         9832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_586935, whole genome shotgun sequence.
ACCESSION   DASTMK010000227 DASTMK010000000
VERSION     DASTMK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586935"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VFQ79_10085"
     CDS             <1..1298
                     /locus_tag="VFQ79_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0140051.1"
                     /translation="GFTASWGTGKPVIGFMADIDGLPETSQKPGVAYHAPLIENGPGH
                     GEGHNAGQAVNVTAAITVKRLMEKHGIRGTVRVYPGVAEELLGSRTYMVNAGLFRDVD
                     VMLSSHISTEFTTTYGGPAGSGLVSTQFTFQGRSAHGASAWNGRSALDAVELMNIGWN
                     FRREHIRPEHRSHYNIVFGGNQPNVVPSEATVWYFFREWDYDRIKELHEIGRRIANAA
                     AMMTDTTVSERVLGAAWPGHFNKPVAEALSANIKRVGMPAWSEADQKLARAAQTELKV
                     KVDGLRTEIPELKPAGDRGSAGSDDIAEVGWNLPTVVLRYPGNIPGMIGHHWSSGIAM
                     ATPIAHKGATAGAKAQAMTALDLLVNPELLKAAKDYFAEQTKDVKWQSLIPTGTEPPI
                     FLNKEKMERFRPEMKKNYYDPAKYKTYLEQLGIEYPTVR"
     gene            1310..2140
                     /locus_tag="VFQ79_10090"
     CDS             1310..2140
                     /locus_tag="VFQ79_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0140052.1"
                     /translation="MPRNLFRILAAAALTFLPALAQTSQPAEAKTVAVNGIDMYYEAY
                     GTGDPLVLLHGFSNSGQTWKAVLDDFSRHYRVVLVDMRGHGRSTNPSGVFTHKQSAAD
                     VFALLDAIGVKRFRAMGISSGGMTLLHMATTQRDRPEAMVLIGATTYFPAQAREIMNR
                     NKPEGLREQDLARQRQIHKHGDEQIYMLRRQFHGFKDSYDDMTFRTPQLATIQARTLI
                     VHGDRDEFFPVSIPVEMYESIPNAALWIVPSGGHVPIQGERRPEFVRIALKFLSDSPK
                     "
     gene            complement(2145..2912)
                     /locus_tag="VFQ79_10095"
     CDS             complement(2145..2912)
                     /locus_tag="VFQ79_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0140053.1"
                     /translation="MTVTRRAFLAHSALASLWAAGQPRFRFGHHFYNWDQSFDLELKL
                     RLTAETGWEGFEAKPEEFGVPAAELRKRATALKLSCAAVGGSLKPAIDYAHAAGAGIA
                     RVTVSKDECARWVDYAGERGVILVVHNHIGGIETREQLLRYLDERPGTYACPDTGHLA
                     LCGSDPVQTIRDLGSRCRYVHLKDIDPAKVGTKTGGGDCFWDLGTGAVDLEGVRDALL
                     TVGYKGWVMVERDRRVPDYAASARNMRQVVRKLFERG"
     gene            complement(2972..4627)
                     /locus_tag="VFQ79_10100"
     CDS             complement(2972..4627)
                     /locus_tag="VFQ79_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0140054.1"
                     /translation="MTSRTHTILCTALFAASAAAQAFLPQSNTAPVQNGKSSLEGLVV
                     NALTGAPLTKVSLTLMRGGTALGGIKTESDDQGRFAFRDLDPGHYTLMGDKAGYARQA
                     YGARSNPTSGSALLLSAGQTTTDLVFKMSPGAAIFGKVFDEDADPVTGVSVMIARTLY
                     KNGDAQLVSAGSASTNDLGEYRIGGLTSGTYVVFAAKRTASTGLTAAWNNPAPDGPER
                     DYVTTYYPNSLDPTNAAPVSVTQGAEVGGINIRLAKADTFRVRGKVSGGAEGKRTMAM
                     LAPKIGSKSATGAFTARSAVARGDGSFEIAGVTPGEYTLVSFSGDLSSGMLIGSKDLQ
                     VGREHVSDVALRLGAGGEVTGSIEMEGPEPADFKNLKVTLNVAPGAVNLSAPSSPVSV
                     GGRFALKDVPSAQYQAQVIGAPGNSYVKSVKYNGETRHDGFTFTGDGGELHITLSPAG
                     AQVEGMVLKAENEPALDATVVLIPASGRSSLYREARTDQKGAFSFKGIAPGEYKALAW
                     EDVETAPYKDPEYLKKVDNSAEALSLKENDHKRVSLKVISPKQ"
     gene            4698..5105
                     /locus_tag="VFQ79_10105"
     CDS             4698..5105
                     /locus_tag="VFQ79_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0140055.1"
                     /translation="MQIAVNPSDELPIYRQIMRQIIEAIAGERLKTGEKLPSHRELSE
                     KIVVAPLTVKKAYDELEALGFLETQRGRGTFVCTALPRIDREDQLESIRQQCRKLLSQ
                     ARLAGIRFSELVELLRIADRELTGGGRDEKERV"
     gene            5102..5998
                     /locus_tag="VFQ79_10110"
     CDS             5102..5998
                     /locus_tag="VFQ79_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0140056.1"
                     /translation="MNPVLEFLNISRSFKRGAPVLDGVSFSMAEGEVVGLLGRNGAGK
                     TTLIHIAMGLLSTHGGAVRVFGVSPFDNPVAVKRRVGFVSEEQVLPPRSTIAELITFH
                     RYLFGRWDDVLERQLLDRFALSPSSKIHSLSKGQARQVALLCAVCHRPELLLLDEPAG
                     GLDPVARREFLETSIQLLNREGTAILFSSHHMADVERIGGRIVLLSEGRVAIDRDLDR
                     LREDVCIAMVPRASAPDPAALRNLPGCLRIRAVFEDWHVVIEGQPDDVLGRLQLISPA
                     DNIRCMRVPLEELFIELMGGER"
     gene            6005..7243
                     /locus_tag="VFQ79_10115"
     CDS             6005..7243
                     /locus_tag="VFQ79_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140057.1"
                     /translation="MIHQLIRREPAWKATPWLALASAVAWSWMPSMFGFAILVAGLVA
                     GMPHRHATRLDAGLPIEARDLLLARTFSLMAVIWIPALAGAASIVIVKGFSASASDPL
                     AAAALGTLIAAVAQALRLKELAGPKWLFFLYFAVNPSGLALLKQLDPLVPLICALSGA
                     ALFIRAWVTVPPSFQLTNASVNAWRPARQERRVAPRAAFRWAPVLRSVCSWQYVFFVP
                     WMGYATATGQWLFLPFWLVVAWRGPRNKTRWLWALPVGRRALLWTMMGPILAAHVLGY
                     LAGFRFHQRAAGLTETRVIVVTLAAILGYVMLTILLCVLFDWRGFARIPRRTLHIVFL
                     LLAIIWYLASFALLYFGPEGEEMRDVLGRVGRTAPMGVPALIATATAALIALYWAIER
                     VFSQPDFAGNPRVSDLDAIS"
     gene            7410..7577
                     /gene="rpmH"
                     /locus_tag="VFQ79_10120"
     CDS             7410..7577
                     /gene="rpmH"
                     /locus_tag="VFQ79_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU0140058.1"
                     /translation="MPKRTFQPNNRKRAKTHGFRARMKSKDGRAVLARRRAKGRHKLT
                     VSDERAVRHAN"
     gene            7917..8126
                     /gene="yidD"
                     /locus_tag="VFQ79_10125"
     CDS             7917..8126
                     /gene="yidD"
                     /locus_tag="VFQ79_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU0140059.1"
                     /translation="MQRALIGILQVYKQWISPLLPSACRFYPTCSVYMREAVEKHGVP
                     RGVWMGVRRLGRCHPFHAGGFDPVP"
     gene            8153..>9832
                     /gene="yidC"
                     /locus_tag="VFQ79_10130"
     CDS             8153..>9832
                     /gene="yidC"
                     /locus_tag="VFQ79_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU0140060.1"
                     /translation="MADTPNSQSGSKPGRKELSTEIRLLIAFLLMGAVLFLTPYFYKP
                     AAPPPKTSPAKEIPAEQTKVPEAEPSKPAQPPPMPVPGQISAQTEELLLVETDLYRIE
                     FSNRGGVVRRWTLKGPKYVDSGGRPLELINQEAAAKVGYPFSVDFKGEKPSTDVNSAL
                     FAVKRSDDGLSLEFEFSDGKTALRKSFHFTRDSYLSKVSSEAVQNGVPMPHFLTWRGG
                     FGDPSLEHAAPSQHTVYYDTREAELVLQEAKHGKDGPVTSSGDYSFAGIEDQYFAAVS
                     LPPAGAVVEMRTYGDPVPTAGGSEEMRVGAGIGGGAQNRFTLFVGPKDLDLLRRIEPK
                     LAQLIDFGWFAFLAKPLFLALNWVNDNLAHNYGWAIVIVTVVINFLMLPLRYTSMKSM
                     KKMQSLQPQIKAINEKYKGIGLRDPRKAEQNQEVMALYKQHGVNPTGGCFPMLLQIPF
                     FFAFYKVLTVAIELRGANWLWVTDLSQPEHLPIRILPITMIITQFIQQKMTPSTMGDP
                     TQQRIMMMMPLMLGFMFYGVSSGLVLYWLTGNVIGIAQQWFFNKTMATPAPV"
BASE COUNT         1943 a         2858 c         2964 g         2067 t
ORIGIN      
        1 cggggttcac tgcgagttgg ggcacgggta agcccgttat cggttttatg gccgacatcg
       61 acgggctgcc tgaaacctcg cagaaacctg gggtggcata ccatgcgccc ttgatcgaga
      121 atggaccggg ccatggggaa ggacacaacg cgggacaggc ggtgaatgtg actgcagcca
      181 tcacggtgaa gcgcctgatg gagaagcacg ggatccgcgg caccgttcgc gtctacccgg
      241 gtgtcgccga ggaactgctg ggcagccgca cttatatggt gaacgccggc ctgttccgcg
      301 acgtcgacgt gatgctgagc agccacatca gcaccgagtt caccaccacc tatggagggc
      361 ctgcgggttc cggcctggtc tcgacgcagt tcacattcca gggccggagc gcccatgggg
      421 cttcggcatg gaacggaaga agcgcgctcg atgccgtgga gttgatgaat atcggctgga
      481 acttccggcg cgaacacata cggcccgagc atcgatcaca ctacaacatt gttttcggtg
      541 gcaatcagcc aaacgtagtt ccatccgagg cgacagtctg gtatttcttc cgcgagtggg
      601 actacgatcg catcaaggaa cttcacgaga tcgggcgccg gattgcaaac gccgccgcga
      661 tgatgacgga tacaaccgtc agcgagcgcg tgcttggcgc cgcctggccg ggccacttca
      721 acaagccggt ggccgaagcg ctgtcagcca atattaagcg cgtcgggatg cctgcctggt
      781 ccgaggcgga ccagaagctc gctcgcgcgg cgcagaccga gttgaaagtg aaggtggacg
      841 ggcttcgaac cgaaataccg gagctcaagc cggccggtga ccgtggcagc gccggttccg
      901 acgacatcgc ggaagtggga tggaatcttc cgaccgttgt actgcgttac cccgggaaca
      961 ttcccggcat gatcggccat cactggtcga gcggcatcgc gatggctacg ccgatcgcgc
     1021 acaaaggagc tacggccggg gcgaaagcgc aggcgatgac agcgcttgat ctgctagtga
     1081 accccgaatt gctcaaggcg gcgaaagatt acttcgccga acagacaaag gatgtaaagt
     1141 ggcagtcgtt gattccgacc ggcaccgaac cgccgatctt tctgaataaa gagaagatgg
     1201 aacgtttccg gccggaaatg aaaaagaatt actacgatcc ggcgaagtac aaaacgtatc
     1261 tggaacagct cgggatagag tacccgacgg tgcgataggg gacgcgtcga tgccccgaaa
     1321 tctttttagg atccttgccg ccgcggcgct tacctttttg ccggcgttgg cgcaaacatc
     1381 gcagcctgcc gaggccaaaa ccgtggccgt caatggcatc gacatgtatt acgaagccta
     1441 tggaaccgga gatcccctgg tgctgttgca tggattcagc aattccggtc agacctggaa
     1501 ggcagttttg gacgacttca gccgccacta ccgggtagtc ctcgtcgata tgcggggcca
     1561 cggacgttcg acaaacccga gcggggtttt cacccacaag caatccgccg cggatgtgtt
     1621 tgcgctgctg gacgcgatcg gtgtgaagcg gttcagggca atgggcatca gtagcggcgg
     1681 gatgaccctg ttgcacatgg cgacgacgca gcgggaccgg ccggaggcga tggttctgat
     1741 tggagcgaca acgtactttc cggcccaggc gagagagatc atgaacagga ataagccgga
     1801 ggggctccgt gagcaggacc tggcgcgcca gcggcagatc cataagcacg gagacgagca
     1861 gatctacatg ctgcgccggc agtttcacgg tttcaaggac agctacgacg acatgacctt
     1921 tcgcacgccg cagcttgcca ccattcaggc ccgcacgctg attgttcatg gcgatcggga
     1981 cgagttcttc cctgtttcta tcccggtgga gatgtacgaa tccattccaa acgcggcgct
     2041 ttggattgtg cccagcggcg ggcacgttcc gatccagggt gagcggagac ctgagtttgt
     2101 gcggatcgcg ctgaagttcc tgtcggacag tccgaagtga gggttcaacc tcgctcgaaa
     2161 agcttgcgaa ccacctgccg catgttgcgc gcgctcgcag cgtaatccgg cacgcggcgg
     2221 tcgcgttcca ccatcaccca ccctttgtag ccgacggtga gcagtgcatc ccggacgccc
     2281 tccagatcaa cagccccggt gccgaggtcc cagaagcaat cgccgccgcc ggtctttgtg
     2341 cccaccttcg cggggtcgat atctttcaga tggacgtaac ggcagcggga ccccagatcg
     2401 cgaatcgttt gcacgggatc ggagccgcag agcgccaggt gccccgtatc ggggcaggcg
     2461 tacgtgccgg gccgctcgtc gaggtagcga agcaactgct cgcgcgtctc gatgccgccg
     2521 atgtgattgt gcacgacaag gatgacgccg cgctcaccgg catagtccac ccagcgggcg
     2581 cattcgtcct tcgaaaccgt aacgcgagcg atgccggcgc ccgccgcatg ggcgtaatcg
     2641 atggcgggct ttaaggagcc cccaaccgcc gcacaggaga gttttaacgc ggtggcccgc
     2701 ttgcgcagtt ccgccgccgg gacaccgaac tcttccggtt tggcttcaaa gccctcccag
     2761 ccggtttccg cggtgaggcg cagcttcaac tccaggtcga acgactgatc ccaattgtag
     2821 aaatggtgac caaagcggaa gcggggttgt ccggccgccc acagcgaagc cagggcggag
     2881 tgggccagaa atgcgcgtcg cgtgaccgtc atggtttgaa gttaccaaga atcggggtgg
     2941 gcgtagctgg gatcggtttt ccgtactcca tttactgctt tggggaaatc actttaagac
     3001 ttactcgctt gtggtcgttc tctttgagcg ataacgcctc cgcgctgtta tcaacctttt
     3061 tcagatactc gggatccttg taaggcgcag tttcgacgtc ttcccacgcg agcgctttgt
     3121 actcgcccgg cgcaatgccc ttgaaactga aggcgccctt ctggtcagtc cgggcttccc
     3181 tatagagcga agaccggccc gaggcgggaa taagcacaac tgttgcatcc aacgcgggct
     3241 cgttctcagc tttcaacacc atgccttcca cctgtgcacc agcgggactc aacgtgatgt
     3301 gcagttctcc cccgtcgcca gtgaaagtga agccgtcatg gcgggtctct ccgttgtact
     3361 ttaccgattt cacgtaggaa ttgccgggcg cgccgatcac ctgggcctgg tactgcgctg
     3421 acggcacatc tttcagagcg aatctgccgc caacgcttac gggactactg ggagcgctga
     3481 gattcacagc cccaggagcg acgttcagtg tgactttcag attcttgaaa tcggcaggct
     3541 ccgggccctc catttcaatc gagcccgtga cctcaccgcc cgctccgagc cgcaacgcta
     3601 catccgaaac gtgttccctg cccacttgga gatccttgga cccgatgagc ataccggaac
     3661 tcaggtcccc cgagaaactc actagagtgt attcccctgg tgtaacgccc gcaatttcga
     3721 agcttccatc accacgggcc acagcgctcc tggcggtgaa ggcgcccgtc gcagatttcg
     3781 agccgatctt tggtgccagc atggccatcg tccgcttgcc ttccgccccg ccgctcacct
     3841 tgccccgcac gcgaaaggta tccgcctttg ccagccggat gttgatgccg cccacttccg
     3901 cgccctgtgt caccgacacc ggcgctgcgt tggttggatc caacgaattc ggatagtacg
     3961 tggtcacata atcgcgctcg ggaccatccg gagctgggtt attccatgca gcggtgaggc
     4021 cagttgatgc agttctcttg gctgcgaaga ctacataagt gccggatgtg aggccgccta
     4081 tgcggtactc gcccaagtcg ttcgtagatg ccgaaccagc cgacaccaac tgcgcgtccc
     4141 cgttcttgta taaggtcctg gcgatcatca ccgaaacgcc tgtcacgggg tcggcatctt
     4201 catcgaacac cttgccgaag atggctgccc ctggtgacat cttgaagacc aggtccgtcg
     4261 ttgtctggcc cgccgagagg agtagcgcgc tgcccgaagt cggattgctc ctggcgccgt
     4321 atgcctgccg cgcataacca gccttgtcgc ccatgagggt gtagtgccca ggatcgagat
     4381 cgcgaaaagc gaaacggccc tggtcgtcag attccgtttt tatgcctccc agcgcagtgc
     4441 cgccgcgcat cagcgtcaag gagactttag ttaaaggagc gccggtcaac gcgttcacta
     4501 cgagtccttc caggctgctc ttcccgttct gaaccggcgc ggtgttgctt tgcggcagga
     4561 acgcctgggc ggcagccgag gcggcgaaca atgccgtgca gaggattgtg tgggttctac
     4621 tcgtcatgaa ggtcctccat atgacaacca tcagttgaca accacagtga catagtgata
     4681 tagaactata tatcactatg caaatcgctg tcaatccctc tgacgagctt ccaatctacc
     4741 ggcagatcat gcggcagatt atcgaggcta tcgctggcga gagattgaaa accggcgaga
     4801 aattgccctc gcaccgcgag cttagcgaaa agatcgtagt tgcgccgctc accgtgaaaa
     4861 aggcttacga cgaactggaa gcgcttggct ttctcgaaac acagcgcggg cgcggcacat
     4921 tcgtatgcac cgcgctgccc agaatcgatc gcgaagacca gctcgaaagt atccgccagc
     4981 aatgccgcaa gctgctttcg caggctcgcc ttgctggcat tcgtttttcc gagttggtgg
     5041 aacttctcag aatcgccgat cgggagctta caggcggtgg gcgcgatgaa aaagagagag
     5101 tatgaaccct gttcttgagt ttctgaacat ctccagatcc tttaagagag gagcgccggt
     5161 cctcgacggc gtcagcttct cgatggccga aggggaagtc gtcgggctgt tgggccggaa
     5221 tggcgcaggt aagacgacgc tgatccacat cgctatgggg ttgctttcca cgcacggtgg
     5281 agcagtgcgc gtcttcggcg tttcgccttt cgataacccg gtcgcggtca agcggcgtgt
     5341 cgggttcgtt tcagaggagc aggtcttgcc gccacgatcc accatcgcgg aactgatcac
     5401 atttcaccga tacctcttcg gccggtggga cgatgtgttg gaacgccagc ttctggaccg
     5461 ctttgcgctt tcgccatcct ctaagatcca cagtctgagc aagggtcagg cgcgtcaagt
     5521 cgcactgctg tgcgcggttt gccaccgccc agagctgctc ttgctggatg agcccgccgg
     5581 cgggctggac ccggttgccc ggcgcgagtt tctggaaacc tcgattcaac tgttgaaccg
     5641 cgagggcacg gcgatattgt tctcgtcgca tcatatggcg gacgttgagc ggattggtgg
     5701 acgcatcgtc ctgctcagtg agggtagggt ggccattgac cgcgacctgg atcgccttcg
     5761 tgaggacgtc tgcatcgcca tggtcccgcg cgcttcggcg ccggaccccg ccgccctcag
     5821 aaacctgccc ggctgtctcc ggatacgagc ggtgttcgaa gactggcatg tcgtaatcga
     5881 gggccaaccc gacgacgtac tcggccggtt gcagttaata tcccctgctg acaacattcg
     5941 ctgtatgcgg gtcccgctcg aagagttgtt tatcgaattg atgggagggg agcgctgagg
     6001 agccatgatc caccaactca tccggcgcga accagcgtgg aaagccacac cgtggctcgc
     6061 gctcgcaagc gcggttgcct ggtcctggat gccgtctatg ttcggctttg cgatcctcgt
     6121 tgcaggtctc gtggccggga tgccgcaccg gcacgccacc cggttagatg cagggctgcc
     6181 gatcgaagcg cgcgatcttc ttctggcgcg cacgttttct ctgatggcgg tgatttggat
     6241 accggcgctc gcgggagccg ccagcatcgt gatcgtaaaa gggttctcgg cgtcggcgtc
     6301 ggatcccctg gcggcggccg ccctgggtac cttgattgcc gccgttgcgc aggccttgcg
     6361 gttaaaggaa ttggcagggc caaagtggct ttttttcctc tactttgctg taaatccctc
     6421 cggtttggcg cttttgaagc agctcgaccc gctcgttccg ctgatttgtg ctttgtccgg
     6481 cgccgcgctg ttcatccgtg cctgggtgac ggtcccaccg tcgtttcagt tgaccaatgc
     6541 aagcgtgaac gcctggaggc cggccaggca ggaaaggagg gtcgccccac gagcggcgtt
     6601 ccgatgggcg ccggtcctcc gatcggtctg ttcctggcaa tatgtgttct tcgtcccctg
     6661 gatgggctat gcgacagcca ccggccaatg gctgttcctc cctttttggc tggtggttgc
     6721 gtggcggggt ccgcgtaaca aaacacgctg gctctgggcg ctcccggttg gaaggcgcgc
     6781 tcttttgtgg accatgatgg ggccgatact cgccgcccat gtgcttggat atcttgccgg
     6841 cttccgcttc catcaacggg cggcagggct tactgaaacc cgggtcatcg tcgtcacgct
     6901 ggcggctatt ctcgggtatg tgatgcttac gattctgctc tgtgtgctct tcgattggcg
     6961 gggttttgcc cgcattcccc gccggaccct tcatatcgtg ttcctgttgt tggccatcat
     7021 ttggtacctg gcgagctttg cgctcttgta tttcggcccc gaaggcgagg agatgcgcga
     7081 cgttcttggg cgtgtgggcc gcactgcacc tatgggcgtg cctgcgctga ttgcgaccgc
     7141 gacggcggct ttgatcgcac tttattgggc catcgagagg gtgttcagcc agcctgattt
     7201 cgccggtaat ccccgtgtga gcgaccttga cgcaatttca tagaaacggc gacaggtaga
     7261 aagtgaggcc cagtcggcgg tgtgcgggcg gattgacagg ctttcggtac gattcctata
     7321 ctgaattatt gggtcactcc caaatactgc gctaaccttg cgcctcgagc gggtcgcttg
     7381 ccccggcgct agaacctgag ataacaaaga tgccgaaaag aacgttccag ccgaataacc
     7441 gcaaacgggc caagacccat ggttttcgag cgcgcatgaa gagtaaagat ggccgtgccg
     7501 tgctcgcacg gcgccgggcc aagggccgtc acaagctgac cgtgagcgac gaaagagcag
     7561 tccgccacgc caactaagtc cgatactgga tttccttttt ccaggagttc ccggctattg
     7621 cggtcggccg agtttcgggg tgtctattcc aaggggatgc ggttctcgag ccgattgttc
     7681 actgccttct gtttggctac agaagggcag gagggaccga aagtgggttt taccgtgccc
     7741 cgcgctttgg ggaactcggt acagagaaac cggatcaaac ggaggatgcg cgaggcggtc
     7801 cggctgcacc tgccgctcat cgggcggaag tgggcgatca tattcaatcc gaggcgtacg
     7861 gcacttacgg cgccttttga cgaactacag cacgaggtcc agaggatagt ccagcgatgc
     7921 aacgcgcgct gatcgggata ctgcaagtct acaaacaatg gatttcgccg ctgctgcctt
     7981 ccgcctgccg attctatccg acgtgttccg tatacatgcg ggaagccgtg gaaaagcacg
     8041 gtgtgccgcg cggcgtatgg atgggtgtaa ggcggcttgg ccgctgccat ccgtttcatg
     8101 cgggtggctt tgatcccgtg ccgtgagtga attggagatc gtaattacgt agatggccga
     8161 cacgccaaat agtcaatccg ggtcgaagcc tggcagaaaa gaattatcca cggaaatacg
     8221 gctcctgatt gctttcctgc tgatgggtgc cgttcttttt ctaaccccct atttctataa
     8281 gcccgccgcg ccgccgccga agacatctcc cgctaaagaa attccggcgg agcagacaaa
     8341 agtgccggaa gcggagccgt ccaaaccagc ccagccccct ccgatgcccg ttccggggca
     8401 gatttcagcc cagaccgaag aacttttact ggtcgaaacc gacctgtacc gcatcgagtt
     8461 ctccaaccgc ggcggcgttg ttcgaaggtg gacgctgaaa gggccgaaat acgtagacag
     8521 tgggggccgc ccgctggagc tgatcaatca ggaggcggcg gcaaaggtcg gttacccgtt
     8581 ttccgttgat ttcaaagggg aaaagccctc gacggatgtg aacagcgcac tttttgccgt
     8641 gaagcgctcc gatgacgggt taagcctcga atttgagttt tccgacggta agacggcact
     8701 gcgtaagtcg tttcacttca ctcgcgacag ctacctgtcg aaggtgtcct cggaagccgt
     8761 ccagaatggc gttcccatgc cgcatttcct cacctggcgg ggaggattcg gcgatccgtc
     8821 cctggaacac gccgctccct cccagcacac ggtttactac gacaccaggg aggctgagct
     8881 cgtattgcag gaggccaagc atggtaaaga tggccccgtg acttcgagcg gcgattactc
     8941 ttttgctgga atcgaggatc agtatttcgc ggcggtctcc cttccacccg cgggagcagt
     9001 cgtggagatg agaacgtatg gcgatccggt tccgacggcg ggcggcagcg aagagatgcg
     9061 tgtgggggcg ggtatcgggg gtggtgcaca gaaccggttc acgttattcg ttggaccgaa
     9121 ggatctggat ctactccgcc gtatcgagcc aaagcttgcg caattaatcg attttggttg
     9181 gttcgctttt ctggccaagc cactatttct ggcgttgaac tgggttaacg ataacctggc
     9241 gcacaactac ggctgggcca tcgtgattgt gacggtcgtc atcaacttcc tgatgctgcc
     9301 actgcgctac accagcatga agtcgatgaa gaaaatgcag tcgcttcagc cccagatcaa
     9361 agccatcaac gagaagtaca agggaattgg tctgcgcgac ccacggaaag ccgaacagaa
     9421 ccaggaagtc atggcgctct ataagcagca cggcgtcaat ccgaccggcg gctgcttccc
     9481 catgctgtta cagattccgt ttttctttgc tttctataag gtattgaccg tcgcgattga
     9541 gctgaggggg gcgaactggc tatgggtcac cgatctgtcg caacccgaac acctgcctat
     9601 tcgaattctt cccatcacca tgatcattac gcagttcatc caacagaaga tgacgccgag
     9661 tacgatgggc gatcctacac agcagcgcat catgatgatg atgcccctta tgctaggctt
     9721 catgttttac ggggtttcga gcggcctggt gttatactgg ttgaccggca acgtgatcgg
     9781 catcgcgcaa caatggtttt ttaacaaaac catggcgacg cctgctcccg tt
//
LOCUS       DASTMK010000228         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_299349, whole genome shotgun sequence.
ACCESSION   DASTMK010000228 DASTMK010000000
VERSION     DASTMK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299349"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFQ79_10135"
     CDS             <1..219
                     /locus_tag="VFQ79_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140061.1"
                     /translation="TRQGKLLLIENYGLSENQGRYTVKRYRSEKQSTEEGWRHTKIVM
                     EPLNPEFERWELEPGDFRVIGEFVRVLY"
     gene            complement(286..>2052)
                     /gene="acnA"
                     /locus_tag="VFQ79_10140"
     CDS             complement(286..>2052)
                     /gene="acnA"
                     /locus_tag="VFQ79_10140"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU0140062.1"
                     /translation="TIGNMAPEYGATIGVFPIDHATLSYLRVTNRPADLVALVEAYAK
                     EQGLFLTPDSPEPVYSETMELDLGDVVPSLAGPRRPQDRINLPDVKKNFLSILGEAPR
                     QSRVSFNGHQETIQDGTVVIAAITSCTNTSNPSVMLAAGLLAKKAVEKGLKPKPWVKT
                     SLAPGSKVVMDYYRDAGLLEYLEALNFHLVGYGCTTCIGNSGPLLENVADAVQKDKLV
                     VAAVLSGNRNFEGRINPLVRANYLASPPLVVAYAIAGRMDINLDTDSLGTDRQGKDVY
                     LKDIWPSPEEVQKVVAGSVRAEMFEKEYSQVFEGDDQWKSMKTPTGELFQWQADSTYV
                     KHPPYFEQMVEPGKALRDFRGLRVLAMLGDSITTDHISPAGSIAVDSAAGKYLIEQGV
                     KPADFNSYGSRRGNHEVMVRGTLGNIRLRNQLAPGTEGAWTTHYPSGEQMWIYDASVR
                     YAAEGVELIIIAGKEYGSGSSRDWAAKGVQLLGVKAVIAESYERIHRSNLVGMGVLPL
                     QFQAGQTRESLGLTGTERYSIEGVADARKDGSRAVIRAVGDDQKELTFEAIVRIDTPP
                     EWEYYNSGGILPYVLRQLAEAK"
BASE COUNT          410 a          618 c          589 g          435 t
ORIGIN      
        1 acacggcagg gaaaactgct gctgatcgag aactacggac tgtcggaaaa ccagggccgc
       61 tacacggtga agcggtatcg aagcgagaaa cagagcaccg aggaaggatg gcggcacacg
      121 aaaatcgtca tggagccgct caaccccgaa ttcgaacggt gggaactcga accgggcgat
      181 ttccgcgtca tcggtgagtt tgtaagagtt ctatattagt ttgcgcgacg atttgattgg
      241 ccacggatgg acacagatcc gtgtgaatcc gtgtccatcc gtggctcatt ttgcttcagc
      301 aagttgccgc aaaacatagg gcaggatacc gccggaattg taatactccc actccggcgg
      361 ggtgtcgatc cgcacgatcg cttcgaacgt taattccttc tgatcgtcgc cgacagctcg
      421 aatcaccgcg cggcttccgt ccttcctggc gtccgccaca ccttcaatgc tgtagcgctc
      481 tgtgccggtc agacctaacg actcgcgcgt ctggcccgcc tgaaattgca gcggcaacac
      541 acccatccca accaggttgg agcggtggat gcgctcgtag ctttcggcaa tgaccgcctt
      601 cacgccaagc agttgaaccc ccttggcagc ccagtctcgc gacgaaccgg acccgtattc
      661 tttgccagcg atgatgataa gctcgacgcc ctccgccgcg tatcgaaccg atgcatcata
      721 aatccacatc tgttcgcctg aagggtagtg agtggtccac gcgccttccg tgcccggagc
      781 gagttggttc cgcaggcgaa tgttgcccaa cgtcccgcgc accatcacct catgatttcc
      841 gcggcgcgag ccgtaggagt tgaaatcggc gggcttcacg ccctgttcga taaggtattt
      901 tccggcggcg ctatcgacag cgatggaacc ggcgggcgag atgtggtcgg ttgtgatgga
      961 atccccaagc atcgccagga cgcgcagtcc ccggaaatcc cgcaacgcct tgcctggttc
     1021 caccatctgc tcaaagtaag ggggatgctt gacgtaggtg gagtccgcct gccactggaa
     1081 gagttcgcca gtaggcgtct tcatcgattt ccactgatcg tcgccttcga aaacctgcga
     1141 gtattccttc tcaaacatct cggcgcgcac cgaaccagcg accacttttt ggacttcctc
     1201 cgggctgggc cagatatctt tcaggtaaac gtccttgccc tgccgatctg ttccgaggga
     1261 gtccgtgtcg agattgatat ccatgcggcc ggcaattgcg tatgccacca ccagtggagg
     1321 agacgcgagg tagtttgccc ggaccagcgg attgatacgg ccttcgaagt tccgattgcc
     1381 gctgagaacg gcggccacta cgagtttgtc tttctgcacc gcgtcggcga cgttttcaag
     1441 cagcggacca ctgtttccga tgcaggtcgt gcagccataa ccgaccagat ggaagttcag
     1501 cgcctccagg tactccagca aacccgcgtc gcggtaataa tccatcacga ctttcgaacc
     1561 cggcgcgagg ctggtcttca cccacggctt cggtttcaaa cccttttcga cggccttctt
     1621 cgccaggagc cccgctgcca gcatcaccga cggattcgac gtgttcgtgc aactggtgat
     1681 cgccgcgatc accaccgtgc cgtcctggat cgtttcctgg tggccgttga agctcaccct
     1741 cgattgtctt ggtgcttctc cgaggatcga gaggaaattc ttcttcacgt cgggcaggtt
     1801 gatgcggtcc tgcgggcgcc tggggccggc aagcgacggc acgacatcgc cgagatccag
     1861 ctccatcgtt tcggagtaga ccggctcggg tgaatccggg gtgaggaaaa gaccctgttc
     1921 cttggcgtag gcttccacaa gagcgaccag gtccgccgga cggttcgtca cacgcagata
     1981 gctgagcgtt gcgtgatcaa tggggaatac gccgatggtg gcgccatact ccggggccat
     2041 gtttccgatg gt
//
LOCUS       DASTMK010000229         5442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_553827, whole genome shotgun sequence.
ACCESSION   DASTMK010000229 DASTMK010000000
VERSION     DASTMK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553827"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..2165
                     /locus_tag="VFQ79_10145"
     CDS             123..2165
                     /locus_tag="VFQ79_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEU0140063.1"
                     /translation="MRRLVPILLVCVSALPAQVFRAGAHEVDVSPTKFPILLSGSFLA
                     RYGDRTEGSICARALALDDGRNRVVLVVSDTLMIPRDLADRAKLRASRTTGVPPVRML
                     ISATHTHSTPPLMGALGTPEEPEYARLFEDRLVEAIEGAVKRLQPARVGWTVTENFDH
                     THCRRWILRPDHMRKDPFGDLTVRAHMHPGYQNPEFVGPAGPVDPDLSLLSVQTAKGE
                     PLALLANYSMHYVGVPGRVASADYYGPFVENMRRKIGPSLVAMMSQGTSGDLHWMDYS
                     RPRVEMNIQKYADSLAEVAYRAYRKIQYKPWVSIAMRETSLKLSRRLPDDKRLAWAKA
                     EVARLGGKLPSNQPEVYACEQVYLASPLRELVLQAVRIGELGITAIPNEVFGITGLKI
                     KAQSPLAHTMNIELANGAEGYIPPPEQHKLGGYTTWPARTAGLEVEAEPRIANAVLGL
                     LEQVSGKPRRSVPHINGTYAKAVLASRPSAYWRGSEFAGPLAKDASGNGHSGNYEDGV
                     AFYLEGPQSPAFSGSVINRAPHYAGGRMKAEVKDLPENYTAEFWFYSGLPAGVRPVTG
                     YLFSRGADTLAVNGNGQIQFSNGDTTLAGRTPVALRTWTHAALVRSGDAVCVYLNGRE
                     EVSGKAAPAGGDGIFVGGRPAGDSNFEGRIDEVALYGRVLSPAEVAKRAGSLSARQ"
     gene            2171..2929
                     /locus_tag="VFQ79_10150"
     CDS             2171..2929
                     /locus_tag="VFQ79_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0140064.1"
                     /translation="MGRTALVCSAGGMFGAYQAGVWEVLVDTFTPDLVVGASIGALNA
                     WAIAGGCPPEELRDLWLNVPEAAAYRWRVPRNPLGGIIDANALEGRIQNLHARFRPRM
                     PIGIVITDLLRLRPILVQNADITWEHLAASCAVLGVLPQVRLNGRMFSDGGLLGALPL
                     WAAVEMGADRIVAINAMPRMPAYVRTAVSTLRALSRHQPPDPVNVEVTAISASARLGG
                     VRDAVFWNRDNADRWLKLGRRDAFELKHSIPSMF"
     gene            2970..3260
                     /locus_tag="VFQ79_10155"
     CDS             2970..3260
                     /locus_tag="VFQ79_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140065.1"
                     /translation="MKDTARQQFRWAPHTSGVTFVKPKDYLAGETITAPTPYAAWLAL
                     RDTPSPLRIGDVLEMPDGDLRIYKYVGFEEARWVVPEAKPQEQTSPQPNTAC"
     gene            3423..4442
                     /locus_tag="VFQ79_10160"
     CDS             3423..4442
                     /locus_tag="VFQ79_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU0140066.1"
                     /translation="MKNRVLVRTTLLILAIASGLCAQDLWITLDGGNGPGAGKHVVLI
                     SGDEEYRSEEALPQLARILSQRHGFRCTVLFAIDPSDGTINPDHAGNIPGLDALETAD
                     LMIIATRFRDLPDTQMRHIVRYVESGRPILGMRTATHAFNLKSSEAYRRYSWNSKEWD
                     GGFGRQVLGETWVRHHGQHGKQSTRGILAQGQERHPILRGIRDGDIWGPTDVYEVTLP
                     LPGDSVPLVMGQVLEGMQPDDPPVAGKQNDPMMPVAWTKTYTGAQGKKARIFTMTMGA
                     SQDLESEGVRRLLINAVYWAVGLEKKIPARSNVDIIGHYKPLPFGFGGFRKGVKPADL
                     VHQSP"
     gene            4439..>5442
                     /locus_tag="VFQ79_10165"
     CDS             4439..>5442
                     /locus_tag="VFQ79_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140067.1"
                     /translation="MSTPPTAPLASAASRRLDKLYEFDREPVSPDRLLGAGYFAGSFA
                     GEHVAATEFVIGVTFVNWGAGIRDIFLGLAIGNLLAVLTWTLLCAPIAVQTRLTLYWY
                     LRRIGGPVLTVVYNVLNAILFCILAGCMITVSASAIRIPFGIPAQVDWYPTDARFVLL
                     VLAVGAVVVAVAILGFKKLASFSSVCSPWMFLMFIAGAVVMLPRLAQEAGLESVNSFQ
                     SFWEIGHRVVWTGQTPDGSPPLGFWKVTAFAWICNLAMHAGLSDMALFRYAKKASYGW
                     CSAFGMYLGHYLAWICAGMMGVGAGLLLSRPLHTLDSGGVAYEALGWAGVLAVIAAGW
                     T"
BASE COUNT         1008 a         1614 c         1706 g         1114 t
ORIGIN      
        1 gcaaatcgcg cgtcgtcgcc atacagcttg gtcacgaccg catggcgcat tatcatcccg
       61 gataccagcg gctggtcaag aacgccatcc tctgggccgg gggccaggag tagggcgagg
      121 ttatgcggcg tctcgttccg atcctgctgg tgtgtgtctc cgctctaccg gcacaggtat
      181 ttcgcgccgg tgcgcacgag gtggacgtct cacctaccaa attcccgatt ctgttgagcg
      241 gctcgttcct ggcccgctat ggggatcgca ccgaaggaag catctgcgca cgcgccctgg
      301 cgctggacga tggcaggaat cgtgttgtcc tcgtggtctc ggacaccctc atgattccgc
      361 gcgatctcgc ggacagggcc aagctgcggg cctcgcgcac aaccggcgtc cctcccgtgc
      421 gaatgttgat ctcggcgacg catactcatt ccactccgcc gctgatgggc gcgttaggaa
      481 cacccgaaga accggaatac gcgcgcctgt ttgaagaccg cctggtggag gcgatcgaag
      541 gcgccgtaaa gcgtcttcaa ccggcgcgtg tgggctggac cgtgacggag aatttcgatc
      601 acacccactg ccgccgctgg attctacgtc ccgatcacat gcggaaagat cccttcggcg
      661 atcttaccgt gcgggcccat atgcatcccg gctatcagaa ccctgagttt gtggggcctg
      721 ccggacctgt cgatccggac ctctccctgc tttccgtaca gaccgcaaaa ggcgagccac
      781 tcgctttgct cgcgaattat tcgatgcact acgtgggtgt gcctggccgt gttgcttcgg
      841 cggattacta tggcccgttt gtcgaaaaca tgcgccggaa aatcgggccg tccctggtgg
      901 cgatgatgtc gcaaggaacc agcggcgacc tgcactggat ggattacagc cgcccccgcg
      961 tggagatgaa catccagaaa tatgccgatt cgctggcgga agtggcatac cgcgcgtatc
     1021 ggaagattca atataagccc tgggtgtcaa tcgccatgcg cgagacttcg ctgaagctct
     1081 cgcgccggtt gccggacgac aaacgactgg cgtgggcgaa agccgaggtc gcgcggctcg
     1141 gcggcaaact gccgtcgaat cagcccgagg tgtacgcctg tgagcaggtc tacctggcat
     1201 ctccgctgcg ggaactggtg ttgcaggctg tgcgcattgg cgagttgggc atcacggcga
     1261 tcccgaatga agtattcggc attaccggac tgaagatcaa agcgcagagt ccgctggcgc
     1321 acaccatgaa catcgagctg gccaacggag ccgaaggtta catccctcct ccggagcagc
     1381 acaagctggg cggctacacc acctggcccg ctcgcacggc agggctggaa gtggaagctg
     1441 agcccaggat cgcgaacgct gtgctcgggc ttctggaaca ggtgtccggt aagccgcgcc
     1501 gctctgtgcc gcacatcaac ggaacctatg cgaaggcggt gctggcttcg cgcccctcgg
     1561 catactggcg cggcagtgag tttgcggggc ctttggcgaa ggacgcctcc ggcaacggcc
     1621 attcgggcaa ctatgaggac ggcgtcgctt tttatctgga aggtccgcag tcccctgcct
     1681 tttcgggcag cgtcatcaac cgcgccccgc actatgcggg cggccggatg aaggccgagg
     1741 tgaaagactt gcccgaaaac tacacggccg aattctggtt ctatagcgga ctgcccgccg
     1801 gggtcagacc agtcacaggg tacctgtttt cacgcggcgc ggacacactc gcggtgaatg
     1861 gaaacgggca gatccaattc tcgaacggag acacaacgct tgcaggccgc actccggtgg
     1921 cgcttcgcac ctggacccac gcggcgctgg ttcgcagcgg cgatgccgtg tgcgtttatc
     1981 tgaacggccg cgaggaggtt tccggcaaag ccgcaccggc cggcggagat ggaatctttg
     2041 tcggaggccg gcccgcgggc gattcaaatt tcgaaggcag aatcgatgag gtagccctat
     2101 acgggcgtgt tctaagccca gccgaggtcg caaagcgggc agggagtctt tccgcgcgac
     2161 aataaacatt gtgggaagaa ccgcgctggt ctgttcggcg ggaggtatgt tcggcgctta
     2221 ccaggctggc gtctgggaag tcctggtgga cactttcacg ccggacctgg tcgtgggcgc
     2281 ctccatcggc gcattgaatg cctgggccat cgccggtggt tgcccgccgg aggagcttcg
     2341 ggacctctgg ctgaacgttc ccgaagcggc cgcttatcgc tggcgcgtcc cccgaaaccc
     2401 gttgggtgga attatcgatg ccaacgcgct cgaaggccgt atccaaaacc tccatgcccg
     2461 cttccggccg cgtatgccga taggaattgt cataacggat cttctgcgcc tccggcccat
     2521 actcgttcag aacgccgata ttacatggga gcatctggcg gcatcctgcg ccgtgctggg
     2581 cgtgttgccg caagttcggt tgaacggcag aatgttctcc gacggcgggt tgctcggcgc
     2641 acttccattg tgggccgcgg tcgaaatggg agccgatcga atcgtcgcca tcaacgcgat
     2701 gccgcgaatg cccgcctacg tcagaaccgc cgtatccact cttcgcgcgc tttcgcgcca
     2761 tcagccgcct gaccccgtca atgtagaggt aacggccatt tccgcgtccg cccgcctggg
     2821 cggcgttcgc gacgccgtgt tctggaatcg cgataacgcg gaccgctggc tgaagctggg
     2881 acgccgcgat gccttcgagc tgaaacattc gattccatca atgttttgat gcacaatatg
     2941 ggggtatgcc ggaatatcgg attcatgcga tgaaggacac agcccggcag cagttccgct
     3001 gggcgccgca cacgagcggc gttaccttcg tcaaaccgaa ggattacctg gctggcgaaa
     3061 ccataacagc gccaactcct tatgccgcgt ggctggcact gcgcgatacg ccttcgcctc
     3121 tgcgcatcgg ggacgtgctc gaaatgcctg acggcgactt gaggatatat aaatacgtgg
     3181 gctttgaaga ggctcgatgg gtagttccgg aggcgaagcc ccaggaacag acatccccgc
     3241 agccgaatac cgcttgctga cgcgcgcggc tccgcttcct tgcggtcgcg actgggaaag
     3301 gttcgctcga tactgagccg cgactgtgaa ggagcggtct ttgtagagga cggagaatac
     3361 gtgaccgcat ttccggcgcc atgtccttcg ccacccgtct gatacgtagt aacatcagac
     3421 ggatgaagaa tcgggtgctt gtgcgcacta cgttgcttat cttggcgatt gcgagtggtt
     3481 tgtgcgcgca ggatctgtgg attaccctgg atggcggcaa cgggcctggt gccggtaaac
     3541 acgtcgttct catcagcgga gacgaggaat accgctccga agaagcgctg ccgcaattag
     3601 ccagaatcct ttctcaacgg cacggtttcc gttgcaccgt gctatttgcc atcgacccat
     3661 cggacggcac catcaacccg gaccacgccg gcaacatccc cggtctcgat gcgctggaaa
     3721 cggccgattt gatgatcatc gcgacacgtt ttcgcgatct gcccgataca cagatgaggc
     3781 atattgtccg ttatgtggaa tccggacggc ccatccttgg gatgcgcacc gccacgcacg
     3841 cttttaatct caagagcagc gaagcgtatc gcaggtattc ctggaacagc aaggagtggg
     3901 acggcgggtt cggaaggcag gtcctcggcg aaacctgggt gcggcatcac gggcagcacg
     3961 gtaagcagag caccagggga atccttgcgc aagggcagga acgccacccg atacttcgcg
     4021 ggatacgcga cggcgatatc tgggggccaa ccgatgtcta cgaagttacg ttgccgctgc
     4081 ccggtgacag cgttcctctc gtgatgggac aggttctgga aggaatgcag ccggatgatc
     4141 ctcccgtggc tggtaaacag aatgacccga tgatgccggt cgcctggacg aagacctaca
     4201 cgggcgctca gggcaaaaaa gcgcgcatct tcacgatgac catgggagct tcgcaggacc
     4261 ttgaaagtga gggcgtgcgg cggctcctta tcaacgctgt ctattgggcg gtgggactcg
     4321 aaaagaaaat cccggccaga agcaacgtcg atatcatcgg ccactacaaa ccgctgccct
     4381 tcggtttcgg cgggttccgg aagggagtga agcccgcgga cctggtacat caatccccat
     4441 gagcactcct ccaacggctc cccttgcctc ggcggcttcg aggcggctgg acaagttgta
     4501 cgaattcgat cgcgaacctg tgtcgccgga ccgcttgctg ggagccggct atttcgccgg
     4561 atcctttgcg ggcgagcacg tggcagccac cgagttcgtg atcggcgtca cgtttgtgaa
     4621 ttggggcgcc ggcatccggg acatttttct cgggcttgcc atcggcaacc ttctcgccgt
     4681 gctgacctgg acattgcttt gcgcacccat tgccgttcag acgcgcctca ccttgtactg
     4741 gtatctgcgg cgtatcggag gcccggttct taccgtcgtc tacaatgtct tgaatgccat
     4801 tctgttttgc attctggccg gctgcatgat caccgtgtcg gcctcggcca ttcgcatccc
     4861 gttcggcatt ccggcgcagg tcgattggta tcccaccgat gcgcgcttcg tgcttctggt
     4921 gcttgccgtg ggagcggtag tggttgcagt cgcgattttg ggtttcaaga aactggcctc
     4981 gttttcttcg gtgtgctctc catggatgtt cctgatgttt attgccggcg ccgtggtgat
     5041 gttaccccgg ctggcgcagg aggccggcct cgaaagcgtg aacagctttc aatcgttttg
     5101 ggagatcggt catcgagtgg tgtggacggg tcagaccccc gacggcagcc ctccgctcgg
     5161 cttctggaaa gtgaccgcct tcgcctggat ctgcaacctc gccatgcacg cgggattatc
     5221 cgacatggcg ctgttccgct atgcgaagaa ggccagctat ggctggtgct cggcgttcgg
     5281 gatgtatctg ggccattacc tggcctggat ctgcgccggc atgatgggcg tgggtgctgg
     5341 attattgctt tcccggcctt tgcacactct ggattcggga ggagttgctt atgaagcgct
     5401 cggctgggcc ggcgtgctgg cggtgattgc agccggatgg ac
//
LOCUS       DASTMK010000230         5077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_202680, whole genome shotgun sequence.
ACCESSION   DASTMK010000230 DASTMK010000000
VERSION     DASTMK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202680"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFQ79_10170"
     CDS             <1..308
                     /locus_tag="VFQ79_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140068.1"
                     /translation="LGAALLSHSSQMQLQGLMYGGTKVQLGLDDVREIFLAVPPLDEQ
                     IRIREEAASQQVKIRALEDKIREAMNRLREYRSALITAAVTGQLDFKKHEKQMEALA"
     gene            305..3343
                     /locus_tag="VFQ79_10175"
     CDS             305..3343
                     /locus_tag="VFQ79_10175"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU0140069.1"
                     /translation="MIDYREKALEDAVEESLVARGGYRTVPESAFDRESALIASEALA
                     FVQATQEKTWKVLAAYYGSRTEAEFIAELRKALDARGTLDVLRHGLEFFGQTFRLAYF
                     APASGLNPDSAKLYAANRLGVTRQVHFDTRNEQSVDLMLSLNGIPVATAELKNHFTGQ
                     TVRDAIAQYRGRDLRGVALFQFKSRALVHFAADPDEVYMTTWLEGESTRFLPFNKGRK
                     QGAGNPDHPGGHRTAYLWEEIWARDSWLDLIGRFLHLEKKEKTDPATGKTRTVESLIF
                     PRYHQIDAVRKIVADARDSGAGKSYLIQHSAGSGKSNSIAWLAHRLASLHDAQDRKVF
                     DSVVVITDRLVLDKQLQETIYQFEHKQGVVVKIDEDSKQLAHALAAGTPIVITTLQKF
                     PFVTNHIGSLPDRRYAVIIDEAHSSQSGEGATRMKALLGGERVREEAKRIAQEEQLPD
                     YEEEVLRVALARRHQPNLSFFAFTATPKYKTLVTFGVRGANGEPEPFHHYSMKQAIEE
                     GFILDVLRHYTTYKVYYRLVKAAGEDPEVERKKATKALARFARLHPHNISQKTEIMVE
                     HYRTHVRPKIGGRAKAMVVTNSRLEAVRYKRAFDKYIGEKGYDFRTLVAFSGTLTDPD
                     DGTGEELTEVNVNGGHISERALPKEFCKDVYGVLIVAEKYQTGFDQPLLHTMYVDKRL
                     GDVQAVQTLSRLNRTCAGKEDTFVLDFVNDPEEIVAAFRPYYADPPVVAEHADPRQLY
                     NLRNDMLGRHVVYEVEVDQFAAVFFAPGGKKDTMSGHALMTSIVDKAVTRFEEAPEKE
                     RGELRDRMEAFRRLYGFLSQVIPFFDTKLEKFDAYLRFLLTKLPRRAGSGPLDLDDEV
                     RLRFYRIQKIQEARLLLEDGQPGRLDAPTEVGTGKAAEEEVPLSRVVDVLNERFGTDF
                     TANDELFWDQVREDAAGDESVRGAAEENTRENFSYVFDKKLEELVMSRLDRNEKQALP
                     FLENEQLRAVLTRILGDQIYELIRGSARRAHGRESIHHTS"
     gene            complement(3438..4562)
                     /locus_tag="VFQ79_10180"
     CDS             complement(3438..4562)
                     /locus_tag="VFQ79_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU0140070.1"
                     /translation="MIQFEVEADRLLFDDTMSFARSQVKKLIESHPDFYPMYTQGGKW
                     KHEGPVWTHWCDGFLGGMMWLFHRHLGDKHPDAGYWKKQAIRYSKPLEPRKFDRDVHD
                     LGFIFMSTYHRWHRVTGEEALKDVLIQAGQTMALRFREQGQYLRSFVADDSLFIDIMA
                     NVGIIFYAARETGDRKLRDVATRHALTTRRVLVRGDGSTAHEGIFDLETGEFLRQSTH
                     QGYRGDSCWSRGLAWAIYGFTACYEYSRDPRFIETAEACADYYITYTPADGIPPWDYN
                     APPESRRQVDTSAAAITAAGMLRLSRLLADPVKGHFYWSTAVHILRSLCEMYLAKGDP
                     SWEGILKGGVYHIHKNLGVNESVMWGDYFFIEALEHALRP"
     gene            complement(4559..>5077)
                     /locus_tag="VFQ79_10185"
     CDS             complement(4559..>5077)
                     /locus_tag="VFQ79_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140071.1"
                     /translation="RIVFNGCRQYAGESFLSFADPPPDSEPAPLTPVEVQLPAGIVLE
                     VELEAPLDLRTAAIGDPVIAIVLRGVRKQGKTLVDKGARLSGRIHKLDRTRSSYFAVG
                     FTFGTLEFGGKRAGIASRVEDAGPFFGLYRANRNSVVQDNTLAVRSTHPMLPAGFRLV
                     LQTIPISNEAKQ"
BASE COUNT         1067 a         1388 c         1695 g          927 t
ORIGIN      
        1 ggcttggagc ggcgctgctg tcacacagtt cccagatgca actacagggg ctcatgtacg
       61 gtggtacgaa agtacagtta ggtctggatg acgttcgcga gatattcctc gccgtgcctc
      121 cactggatga gcagatccga atccgagaag aggccgcttc acagcaggtg aaaatccgag
      181 ccttggagga caaaatacgg gaagcgatga accgccttcg cgaataccgc tcggctttga
      241 tcaccgccgc cgtcaccggg caactggact ttaagaagca cgagaagcag atggaggcgc
      301 tggcgtgatc gattatcgcg agaaggcact cgaagacgcc gttgaggagt cactcgtcgc
      361 tcggggcggg taccggaccg ttccggaatc cgcgtttgat cgcgagtccg cgctgatcgc
      421 gtcggaagcg ctcgcctttg ttcaggccac gcaggaaaag acgtggaagg tgctggccgc
      481 ctactacggc tcgcgcacgg aagcggagtt cattgcggaa ctgcggaaag cgctcgatgc
      541 gcgtgggacg ctcgacgttc tccgccatgg gctcgagttt ttcggccaga cgttccggct
      601 ggcgtacttc gctccggcga gcggtctcaa tcccgatagc gcgaagctgt atgcggcgaa
      661 ccggctcggg gttacgcggc aggtccactt cgatacacgg aacgagcagt ccgtggatct
      721 gatgctcagc ttgaacggca ttcctgtggc cacggcggag ctgaagaacc acttcacggg
      781 gcagacggtg cgggatgcta tcgcgcagta tcgcggacgg gacctacgcg gtgtggcgct
      841 gtttcagttc aagtcgcggg ctctggtgca cttcgccgcc gatccggacg aggtctacat
      901 gaccacgtgg ctcgaagggg agtccacgcg attcctgccg ttcaacaagg gacgcaagca
      961 aggtgcggga aatcccgatc atccgggcgg gcaccggacg gcgtacctgt gggaagagat
     1021 ctgggcccgc gattcgtggc tcgacctgat cgggcggttt ctgcaccttg agaagaaaga
     1081 gaagaccgac ccggcgacgg ggaagacgcg gacagtggag agcctgatct ttccgcgcta
     1141 tcaccagatc gatgcggtgc ggaagatcgt agcggatgcg cgggattccg gggcgggaaa
     1201 gagctacctg atccagcatt cggccggcag cggcaagagc aattcaatcg cgtggctggc
     1261 gcaccggctc gcgagcctgc atgacgctca ggaccgcaag gtgttcgatt cggtggtggt
     1321 gatcacggac cggctggtgc tcgataagca gttgcaggag acgatttacc agttcgagca
     1381 caaacagggc gtggtggtga aaatcgacga agactcaaag caactggcgc acgcgctggc
     1441 tgcggggaca ccgatcgtta tcacgaccct gcagaagttc cccttcgtga cgaaccacat
     1501 cggcagcctg ccggacaggc ggtatgcggt gatcattgac gaggcgcatt catcgcagtc
     1561 gggcgagggg gccacgagaa tgaaggctct gctcggcggg gagcgggtgc gggaagaggc
     1621 gaagcggatc gcacaggaag agcaactgcc ggactacgag gaggaagttc tgcgggtggc
     1681 gctcgcgcgc aggcatcagc cgaacctgag cttcttcgca ttcacggcga caccgaaata
     1741 caagacgctg gtgaccttcg gggtgagggg cgccaatggc gagccggagc cgttccatca
     1801 ctactcgatg aagcaggcga tcgaggaggg cttcatcctc gacgtgctgc ggcactacac
     1861 gacttacaag gtgtattacc ggctggtgaa ggcggccggg gaagatccgg aagtggagcg
     1921 caagaaggcg acaaaggcgc tggcgcgctt cgcgcggttg catccgcata acatttcaca
     1981 gaagacggag atcatggtgg agcactaccg gacgcacgtg cggccgaaaa tcggcgggcg
     2041 cgcgaaggca atggtggtga cgaactcgcg gcttgaagcg gtgcggtaca agcgcgcctt
     2101 cgacaagtac atcggggaga agggctatga cttccgcacg ctggtggcgt tctcggggac
     2161 gctcacggac ccggacgatg ggacgggtga ggagttgacg gaggtaaacg tcaacggcgg
     2221 ccacatttcc gagagggcgc tgccgaagga gttctgtaag gacgtctacg gcgtgctgat
     2281 tgtggccgag aagtaccaga cggggttcga tcagccgctg ctgcacacga tgtatgtgga
     2341 caagaggctc ggcgacgtgc aggcggtgca gacgctgtcg cggctgaatc gcacctgcgc
     2401 ggggaaggaa gacacgttcg ttctggattt cgtgaacgat ccagaggaga tcgtggcggc
     2461 tttccggccg tactatgcgg accctccggt ggtagccgag catgcggacc cgcggcagtt
     2521 gtacaacctg cggaacgaca tgcttgggcg gcatgtcgtg tacgaggtgg aagtggatca
     2581 gttcgcggcc gtgttcttcg ctccgggcgg gaagaaggat acgatgagcg ggcacgcgct
     2641 gatgacgtcc atcgtggaca aggcggtgac gcggttcgaa gaggctccgg agaaagagcg
     2701 gggggagttg cgggaccgga tggaggcgtt ccggcggttg tacggattcc tgtcgcaggt
     2761 gattcctttc ttcgatacga agctggagaa gttcgacgcg tacctgcggt tcctgttgac
     2821 gaagctgccg aggcgggcag gctcggggcc gctggatctg gacgatgagg tgcggctgag
     2881 gttctatcgc attcagaaga tccaggaagc gcggctgctg ctggaggacg gacagccagg
     2941 acgtctggat gccccgacgg aggtggggac aggcaaggcg gcggaggaag aggtgccgct
     3001 gtcgcgcgtg gtggatgtgc tgaacgaacg gttcgggacg gacttcacgg cgaacgacga
     3061 acttttctgg gaccaggtgc gggaggacgc agcgggcgac gagtcggttc gcggggcggc
     3121 ggaggagaat acgcgggaga acttctcgta tgtcttcgac aagaagctgg aagaactggt
     3181 catgagccgg ctggaccgga acgagaagca ggctctgccg tttctggaga atgagcagtt
     3241 gcgagcggtt cttacgcgca ttctgggcga tcaaatatat gagcttatac gtggaagtgc
     3301 ccggcgggcg cacggaaggg aatcgataca tcacacttcg tgatgctacg atgccatgtc
     3361 ccggcagtac tcagcgaggc aaatgggctt tcccgctcga catgcgttga gcacccggcc
     3421 tgacggcgct acggctttta cggccgtaag gcgtgctcca atgcttcgat gaagaagtaa
     3481 tcgccccaca tcacggattc attcacaccc aggttcttat ggatgtgata aaccccgccc
     3541 ttcaaaatgc cttcccagga aggatcgccc ttcgcaaggt acatttcgca tagggagcgc
     3601 agaatgtgga ccgcggtgga ccagtagaaa tgccctttca ccggatccgc gagcaggcgc
     3661 gaaagccgca gcattccggc cgcggtgatc gcagcggcgg aagtatccac ctgtctccgg
     3721 ctttcaggcg gcgcgttgta atcccatggc ggaatgccat cagccggcgt atacgttatg
     3781 taataatcgg cgcacgcctc ggccgtttcg ataaatcgcg ggtcgcggct gtattcgtag
     3841 caggcggtga agccgtagat ggcccaggca aggccgcgcg accagcacga gtcaccgcgg
     3901 tagccctggt gcgtggactg ccggaggaac tcacccgtct ccagatcgaa gatgccttcg
     3961 tgcgccgtcg atccgtcgcc gcgtaccagc acacgccgcg ttgtcagggc gtgccgggtg
     4021 gcgacatcgc gcagtttccg gtcgcctgtt tccctggcgg cgtagaagat gattccgacg
     4081 ttcgccatga tatcgatgaa cagcgaatcg tccgccacaa acgagcgcag atactggccc
     4141 tgttcgcgga aacgaagagc catcgtctgg ccggcctgga tcaggacatc tttcagcgcc
     4201 tcctcgccgg tgacgcgatg ccaccggtga tacgtcgaca tgaagatgaa tccgaggtca
     4261 tggacgtcac ggtcgaactt tctcggctca agtggcttgg agtaacggat cgcctgcttc
     4321 ttccaatacc cggcatccgg atgcttgtcc ccgagatggc gatggaagag ccacatcatg
     4381 ccgccgagga agccatcgca ccagtgtgtc cataccgggc cctcatgctt ccacttaccg
     4441 ccctgagtgt acatcgggta aaagtccggg tgggattcaa tgagcttttt gacctgcgac
     4501 cgggcgaaac tcattgtatc gtcaaaaaga aggcgatcgg cttcaacctc gaactggatc
     4561 actgttttgc ctcattggaa attggaatag tctgcaatac cagccggaaa ccggcaggca
     4621 gcattggatg agtacttcgc accgccagcg tattgtcttg aacgacggaa ttgcgattgg
     4681 cgcgataaag accgaagaat gggccggcat cctccacgcg tgatgcgatt ccggcacgct
     4741 ttccaccgaa ttcgagagtc ccaaaggtaa aacccaccgc gaagtagctg cttcgcgtcc
     4801 ggtccaactt atgaatgcgt cccgagaggc gcgctccttt gtcgaccagt gtcttgccct
     4861 gtttgcggac gccgcgaagc acgatcgcga taacagggtc gccgattgcc gccgtgcgca
     4921 aatccagcgg tgcttccagc tccacctcga gaacgattcc ggcgggcaat tgaacctcta
     4981 ccggcgtgag cggcgcaggt tccgaatccg gtggaggatc ggcgaacgaa agaaaagact
     5041 caccagcgta ctgccggcac ccgttgaaca cgatcct
//
LOCUS       DASTMK010000231         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_107152, whole genome shotgun sequence.
ACCESSION   DASTMK010000231 DASTMK010000000
VERSION     DASTMK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107152"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFQ79_10190"
     CDS             <1..566
                     /locus_tag="VFQ79_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140072.1"
                     /translation="KLLLWNLRYARLALLPAAIVAVPAILIMSQLEAVYGSEPLMPGE
                     TALVTVQLKRGTQPVAADLTAPSGFTVETPPVRIADLRQVCWRIRARGSADGELHFQT
                     AGESLHRAIHAGPGLRYVTPECSSAPLASIWYGCAFESNSVESIAVDYRARKISLFGF
                     DAHWLVWFALFWLAAMLLLRRRFGVTF"
     gene            complement(669..2702)
                     /locus_tag="VFQ79_10195"
     CDS             complement(669..2702)
                     /locus_tag="VFQ79_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0140073.1"
                     /translation="MKTVPRAIPVTLCAVLALVALSCRAPSPERPAEGPASRRLNVVV
                     VTIDTLRADRLGCYGYKAISTPHLDRFASRGALFENAVVQTPLTAPSHASMFTGQNPP
                     AHKVRDTGGFILNPSSTTLAEILQSRGWDTAAFVGAAVLKKGFGFERGFAVYDDRLPA
                     PSSTVFAAESSERRAGDVVDEAIRWLNSQFGKPFFLWVHVFDPHSPYDAPSPFREKYS
                     GRPYDGEVAYTDQELGRLLDAVGRKSPGETTLTAILSDHGESLSEHGEYTHGVFLYDS
                     TVRIPFLIAGPGIPPGLRVKQQARSIDVLPTMLELLGIPIPGTSQGTSLVPAFGGKEF
                     AASPSYLETLFPKINMGWTELRGIRTDRWKYIRAPKPELYDLAADPLESKNVIGRYAA
                     EARELEANLNAAAGSAAHTPEKIQTAMVDPATLRQLRSLGYSAGSSEREFTLTGQGID
                     PKDRTEVLRLLYLAVSPEAGTPVSRRAELLRRALAADSGNPTVYLHLGDEYGRTGRRE
                     DALKLYQDGIRNGVRTAWLYSRLGFLYMAQGNPEQAIAAYERAAQLNPADCDSLNDLG
                     MTYLDAGRPGDAERVFQWCLAAVEDHALSHNGLGLVAVRKDDFAAARGHFEKAVQLDP
                     DLLEGYLNLGRIYKMSGDYAKARQCFETFLAKASPAQYAQIIPRLREELRQMR"
     gene            2792..3844
                     /locus_tag="VFQ79_10200"
     CDS             2792..3844
                     /locus_tag="VFQ79_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0140074.1"
                     /translation="MLGRIVLSVLAVSVALGQTGESAAQSAPAARQQSEPGASQPEIR
                     VQVNEVIVPVTVTDEKGRFVSDLDVGDFQIFDDGKPQTIKFFSRERNQPVVVGFLIDQ
                     SNASRIHWKTYKDAIQELIWALLPHDDKFSGYLISYSTDAELLANTTNDPEKLVEKVR
                     TMKPGGGAALFDAVYMACTSRALVKGEPIEPRRVIVIVGDGHDTSSKHTMAEVVELAQ
                     RNLVTIYGVSTVAFGFNVQGEKNLTRLAEETGGRVEYPLQGVYSDVSGYLSTPSDEGN
                     YALKVGTGGYAAAIAKGIFEAVANVTGEVTTQYVIRYVPEAGDVSRQFRRIEVKVPAL
                     PGVVIRARKGYYPYEL"
     gene            3908..>4485
                     /locus_tag="VFQ79_10205"
     CDS             3908..>4485
                     /locus_tag="VFQ79_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140075.1"
                     /translation="MSSASTVATLHRSDCLFLQVALPGRSPENAGVLLLDVEADRLHV
                     RLRRDWSEMADEEEAEVLELFEHDIQLKANELGADAFLRYLEGSASNILRVTDREPMQ
                     VADFEARLRRLYKEYVNPKVLPYRTHLPVFTLRAAAGKFGGEMEVEPEEWEETPPELR
                     VTPDMFLAHVTGRSMEPRIPDDSLCVFRANVVG"
BASE COUNT          881 a         1342 c         1377 g          885 t
ORIGIN      
        1 agaagctgct gctgtggaac ctgcggtatg cccggctcgc gctgttgccg gcggcgattg
       61 ttgccgtccc cgcgattctg atcatgagcc aactggaagc ggtgtatgga agcgagccgc
      121 tgatgccggg ggagacggct cttgttaccg tgcaactgaa acgcggcacg caacccgtgg
      181 cggccgatct caccgcaccg tccggcttca ccgtggagac acctccggtg cgtatcgcgg
      241 acttgcggca ggtctgctgg cgcattcgtg cacgcgggag cgccgatggc gagcttcact
      301 ttcaaaccgc tggagaaagc ttgcatcgcg cgatccatgc cggccccggc cttcgttacg
      361 tgacgccgga gtgttcgtcc gctcccctgg cgtcgatctg gtacggatgc gccttcgaga
      421 gcaattcggt ggagtccata gccgtggatt accgtgcccg caagatttcc ctcttcggtt
      481 tcgacgcgca ctggttagtc tggttcgccc tcttctggct cgcggccatg ctactgctcc
      541 ggcggcgttt cggcgtgacg ttctgagttg atggaagtcc gaaaaggcca cttgctgacg
      601 cgcgtggctc agtaagacca ttgcgtgaca gagccgtgac cgtccgggag cggtgtttac
      661 cgcggccgct atcgcatctg ccgcagctct tcccgcagcc tggggatgat ctgcgcgtac
      721 tgggcgggcg aagccttcgc tagaaacgtt tcgaaacact ggcgcgcctt cgcgtagtcg
      781 ccggacattt tgtaaatgcg ccccaggttc aggtagcctt cgaggaggtc gggatccaat
      841 tggaccgctt tctcgaaatg cccgcgggcc gctgcaaaat cgtcttttcg caccgcgacc
      901 agccccaggc cgttatggga caaggcatga tcttcaactg ccgccaggca ccattggaag
      961 acccgttcgg cgtctccagg tctcccggca tccaggtagg tcatgccgag gtcgttcagg
     1021 ctatcgcaat cggcaggatt gagttgcgct gcccgttcat aggcggcgat cgcctgctcc
     1081 gggttccctt gcgccatgta cagaaaaccg agccgcgagt agagccacgc cgtccggact
     1141 ccgttgcgaa tgccatcctg gtatagcttc agggcgtctt cgcggcggcc ggtccggcca
     1201 tactcatcgc ccaggtgtaa gtaaacggtt ggatttcccg aatctgccgc cagtgcccgc
     1261 cgcagcagtt cggcgcggcg agagacggga gtgccggctt ccggagaaac ggcaagatac
     1321 agcaggcgga gaacctcggt gcgatctttc gggtcgatgc cctggccggt gagcgtgaac
     1381 tcgcgttccg aagacccggc cgaataaccc agagaccgca actgccgcag cgtggccgga
     1441 tccaccatcg ccgtctgtat cttctcggga gtgtgagccg cactgccggc ggcagcattg
     1501 aggttggctt ccaactcgcg tgcttcggcc gcgtaacgcc cgatcacgtt tttcgattcc
     1561 agcgggtccg cagccaagtc gtacagctcc ggcttcggcg cgcggatgta cttccagcga
     1621 tcagtgcgga tgccccggag ttcggtccag cccatgttga tcttcggaaa caacgtctcc
     1681 aggtaggacg gcgaagccgc gaattcctta cctccgaaag ctggaaccag gctcgttccc
     1741 tgcgatgtgc cgggaatcgg gatgcccaac agctccagca tggtcggtag cacgtcgatg
     1801 cttcttgcct gttgtttgac gcggagacct ggcggaattc cgggcccggc aatcagaaaa
     1861 ggaatccgca ccgtggaatc gtacaggaac acgccgtgcg tgtactcgcc gtgctcggaa
     1921 agactctcgc catggtcgga aagaatggcg gtcagtgtgg tctcccccgg cgacttccgc
     1981 cctactgcat ccagcaagcg gcccagttcc tgatccgtat aggctacttc gccatcgtac
     2041 ggccgcccgg aatatttctc gcggaacgga gacggcgcat cgtacggaga gtgggggtcg
     2101 aatacgtgca cccataaaaa gaacggcttt ccgaactgcg agttgagcca gcgaatcgcc
     2161 tcgtccacca cgtcgccggc gcgccgttcc gaagattcgg ctgcaaacac ggttgaggat
     2221 ggcgccggca gccggtcgtc ataaaccgcg aaccctcgtt caaacccgaa gcctttcttc
     2281 aaaacggccg ctcccacgaa cgccgcggta tcccatcccc gactttgaag gatttcggcg
     2341 agagttgtgc ttgacgggtt gagaataaac ccgcccgtgt cgcgcacttt gtgcgccgga
     2401 gggttctgac ccgtgaacat gctcgcgtga gaaggagcgg tgagaggcgt ctgcaccacc
     2461 gcgttctcaa acagcgcacc tctgctcgcg aagcggtcca gatggggcgt cgagatggct
     2521 ttgtatccgt agcagcccag gcggtccgcc cgcagtgtgt cgatcgtgac gacaacaacg
     2581 tttaatcgcc tggaggccgg cccttcagcg ggtcgctctg gactcggcgc gcggcaggac
     2641 aacgcaacca gcgcgagtac agcgcagagg gttaccggaa tcgctcgggg tacggttttc
     2701 atcgaaattg caagtaccga ttatcgctca ggcagcggaa ccgttcgttg cccgaaccgt
     2761 ctataatcgg agcaggaagc ccaaacagag tatgctgggt cgaattgtat taagcgtcct
     2821 tgccgtttcc gtcgccctcg gccagaccgg ggagtccgcc gcccagtccg ctccggcggc
     2881 gcgccagcag agcgaaccgg gagcctcaca gcccgaaatc agagtgcagg tgaatgaggt
     2941 gatcgtccct gttacggtca cggacgagaa aggccgtttc gtatcagatc tcgacgtggg
     3001 agatttccag atcttcgacg acggaaagcc gcaaacgata aagttctttt cccgcgaacg
     3061 gaatcagccg gtcgtcgtcg gtttcctcat cgatcaaagc aatgccagcc gcattcactg
     3121 gaagacctac aaagacgcta tacaggagct gatttgggcc cttctgccgc acgacgataa
     3181 gttcagcggc tatctgatct cctattcgac agacgccgaa ctgctggcga acaccaccaa
     3241 cgacccggaa aagctggttg agaaagttcg caccatgaag cccgggggcg gagcggctct
     3301 cttcgatgcg gtctacatgg cctgcacaag ccgggcgctg gtgaaaggag agcccatcga
     3361 gccccggcgt gtcattgtga tcgtgggcga cgggcacgat accagcagca aacacacgat
     3421 ggccgaagtg gtcgaactgg cgcaacgcaa cctggtaacc atttacggtg tcagcacagt
     3481 ggcgttcgga ttcaatgtgc agggcgagaa gaatctcacc cggctggctg aggaaaccgg
     3541 cggccgggtc gagtacccct tgcagggagt ctattccgac gtgtccggtt atctttcaac
     3601 gccatccgat gaaggcaatt acgcgttaaa ggtgggtacg ggaggatatg cggcggcgat
     3661 cgcaaagggt atcttcgagg ccgtggcaaa tgtgaccggg gaagttacga cccagtacgt
     3721 tatccgctac gttcccgagg ccggcgacgt ttccaggcag ttccgaagga tcgaagtgaa
     3781 ggttcccgct ttaccgggcg tggtaatccg tgcccgcaag ggctattatc catacgagct
     3841 gtaggactgg tacgtcctcc attttggatt catggaatta gtttgcggtt ccgataagat
     3901 ctaacagatg agcagcgcat cgacggtagc cacactacac cgctccgatt gtctgttcct
     3961 gcaggtggct ttgcctggcc gctcccccga aaatgcaggc gttcttctgc tggacgtcga
     4021 ggcagaccgg ctccacgtac gtctgcggcg tgactggtcg gaaatggcgg acgaagaaga
     4081 agcggaagtc ctcgagctgt tcgagcacga tatccagctt aaagcgaacg aactgggggc
     4141 agacgctttc ctgcggtatc tggaaggcag cgcttccaac atcctccgtg tgaccgatcg
     4201 ggagccgatg caggtcgccg attttgaagc ccggctgcgg cgcttgtaca aggaatacgt
     4261 caacccgaaa gttctgccgt atcggaccca tctgcccgtc ttcacgcttc gggcggcagc
     4321 cggcaagttc ggcggcgaga tggaagtgga accggaagag tgggaagaga cccctccgga
     4381 actgcgcgtt acccctgaca tgttcctcgc ccacgtcacc gggcgctcca tggaaccgag
     4441 aatccccgat gacagcctct gtgtgttccg cgccaatgtg gtcgg
//
LOCUS       DASTMK010000232         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_138862, whole genome shotgun sequence.
ACCESSION   DASTMK010000232 DASTMK010000000
VERSION     DASTMK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138862"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VFQ79_10210"
     CDS             complement(<1..867)
                     /locus_tag="VFQ79_10210"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0140076.1"
                     /translation="MRPSALILTLVCAAAFAQLPEKKAQEGWIMLFDGQTTFGWQNEG
                     GGKWHAEDGVLVGEPEGPDGWLRTTTPFADFVLTLEFRKGEAGNSGVFVRSAKDGAPH
                     ETGYEFQIWDEHPEFPTGSMVNYAPAKRVKLAPGKWHTYEIRALADSFQVSINGRKVL
                     DAKDPKAKFGFIGLQHKEGQRIEFRNVVLRPLGLSSIFNGKDLRGWSKVDTPRAKEPP
                     VWSVKDGLLHVEKGPGQLETEATYDDFILQLDVRANSSDPGHHPNSGVFLRGDPKAFW
                     SGYESQIRNEYKD"
     gene            complement(930..2870)
                     /locus_tag="VFQ79_10215"
     CDS             complement(930..2870)
                     /locus_tag="VFQ79_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0140077.1"
                     /translation="MQTPMGANLVEGGATFRVWAPRARDVYVVAGAEAAASREPGWTP
                     RLSDRLTRREDGTWALFVPGLKEGAPYRFYVVGDAGGGFKRDPYARELGIQPAFPDCD
                     CLVRDPTTYPWHDSSFHAPAFNDLIIYQLHVGAFYGVDAQGRDKREQVADFLDVITRI
                     PYLRDLGVTAIQLLPVQEFPFDTSLGYNNVDFFSPEMAYQVDDIAELNRYLPAVNALL
                     SEQGAPPLTLAQLEPGPNQLKTLIDLLHMNGIAVIFDLVYNHAGGGFDDHCLYFFDMQ
                     NRGDNRDSLYFTNSGWAGGLVFNYQEDGVRDFLIQNARFFLEEYHADGIRYDEVSVID
                     NHGGWFFCQDLTNTLHFVKPEAIHIAEYWNSRRELAVQAPPAGMGFDAALADGLRERL
                     REAVGQAAGGASADVNLYAVRDALYPPYGFNEAWRAVNCLENHDIVYSDREPEEWRPR
                     LAKLADWNNPRSWYARSRSRFATSILLTAPGIPMLFMGQEILEDKNWSDNPAYHKNTL
                     VWWEGLEQDPAMRDFLAFTRALIRLRRDCRALRGPNVNGYHVNHASRVIAFHRWMDWS
                     GEDVIVAGTLSEGSLYDYRIGFPQAGAWREIFNSDFFESSAGHRTTGNEGAVAADGPP
                     LHGMPASARITIPANGVVVFTR"
     gene            complement(2879..4000)
                     /locus_tag="VFQ79_10220"
     CDS             complement(2879..4000)
                     /locus_tag="VFQ79_10220"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEU0140078.1"
                     /translation="MQAAQPVSAAAPLGAVTPPQTPQPRYLALDAYRGFIMIMLVSGG
                     FGLAALAEKNPAFTALANQFRHMPWEWISFWDLIQPAFMFMVGVAMPFAFARRMELGA
                     TNRDLFRHVAVRSVRLILMSQILMSISRGEMHFQLINVLAQIGITYFLCYLIMQLRFR
                     WQAVIAAGILIGHWALFVAFPGSEGPFMSKTTNIGAVIDKFVFGRMNGGYWVNINFIT
                     STATTLFGVWTGKLLMTRSSHAVKMRTLAIAAFLSLLLGLVIHPWNPIIKRICTTSFT
                     LYSTGWVLLMLLAFYWVVEVKEYRAWTFPLVVVGANSIFIYSVDIVLRGWLNRAIGVF
                     TLKFEWLGDFASVAQACAVLLAMWYLCYWLYRRKIFFKL"
BASE COUNT          957 a         1285 c         1321 g          934 t
ORIGIN      
        1 atccttatac tcattgcgga tttgtgactc gtagccgctc caaaacgctt tcggatctcc
       61 gcgcaggaag acgccactgt tcgggtgatg cccagggtcg ctgctattcg cacggacatc
      121 gagctgcagg atgaagtcat cgtatgtagc ttccgtctcg agttgtccgg gacccttttc
      181 aacatgaagc agaccgtcct ttaccgacca gaccggtggt tccttcgcgc gaggcgtatc
      241 taccttgctc cagccgcgga gatcttttcc gttgaaaata gatgatagtc caagcggcct
      301 caaaacgacg ttgcggaatt cgattcgttg tccttctttg tgctgcaagc cgatgaatcc
      361 gaattttgcc ttcgggtcct tggcgtccag caccttcctg ccgttgatgc tcacctggaa
      421 gctatccgcc agcgcccgga tttcgtaggt atgccatttg ccgggcgcaa gcttcacccg
      481 cttagccggg gcataattca ccatgctgcc ggtcggaaat tccgggtgtt catcccagat
      541 ttgaaactcg tagccggtct cgtgcggtgc tccgtctttt gcggatcgaa cgaagacgcc
      601 actattgccg gcttcgcctt ttctgaattc gagtgtgaga acgaaatccg cgaaaggcgt
      661 ggttgtgcgc agccacccat ccggcccttc cggttctcca accagcactc cgtcttcagc
      721 gtgccacttg ccgccgcctt cgttctgcca gccaaaggtg gtttgcccgt cgaacagcat
      781 gatccagcct tcctgggcct tcttttcggg caattgtgcg aatgcggcag cacagacgag
      841 cgtgaggatc agagcagatg gtctcatttc gagaaccatg ctaacaaagt gcggcctcct
      901 gtagcggggc cgcgggtggt ccccggcgct catctggtga acactacgac accgttcgcg
      961 ggaatggtaa tgcgcgctga agccggcatg ccatgaagcg gcggtccgtc cgcggcgacg
     1021 gcgccttcat tgccggtggt tcgatgcccg gcggagcttt cgaaaaagtc gctattgaag
     1081 atctccctcc aggctcccgc ctgcgggaat ccgatccggt aatcgtacag gctgccttcg
     1141 ctgagcgtgc ctgccacgat gacgtcttcg ccggaccaat ccatccagcg gtgaaaagcg
     1201 ataacccgtg acgcatggtt cacgtgatag ccgttcacat ttggcccgcg cagcgcgcgg
     1261 caatcgcggc gcaggcggat cagggctcga gtgaaggcca gaaaatcgcg cattgccggg
     1321 tcttgttcca ggccctccca ccatacgagc gtatttttgt ggtaggcggg gttgtcgctc
     1381 caattcttgt cttcgaggat ctcctgaccc atgaagagca tgggaattcc cggcgcggtc
     1441 agcaaaatgg aggtggcaaa acggctgcgg ctgcgcgcat accacgagcg gggattgttc
     1501 caatcggcga gcttggcgag gcggggccgc cactcctccg gctcgcggtc cgagtacaca
     1561 atgtcatggt tctcgagaca attcactgcg cgccacgcct cattgaagcc gtatggcggg
     1621 tagagggcgt cccgcacggc gtaaaggtta acgtccgcac ttgcgccgcc ggctgcctgg
     1681 ccgaccgctt cgcgcaatcg ttctcgcaat ccgtcagcta gcgcggcgtc gaagcccatc
     1741 ccggctggag gcgcctgtac tgcgagttcc cgccggctgt tccagtactc ggcgatgtga
     1801 atcgcttccg gcttcacgaa gtggagagtg ttggtcagat cctggcaaaa gaaccatccg
     1861 ccgtgattgt cgatgacact gacttcgtca tagcggatgc cgtcggcgtg gtactcctca
     1921 aggaagaatc tggcattctg aatcaggaaa tcgcgaacac cgtcttcctg atagttgaag
     1981 acgagccctc ccgcccagcc gctattggtg aaatagaggc tgtcccggtt atccccgcgg
     2041 ttttgcatgt cgaaaaaata gaggcagtga tcgtcgaaac caccgcccgc gtgattgtag
     2101 acgagatcga agataacggc gatgccgttc atgtgcagca gatcgatcaa ggtcttcaac
     2161 tggttgggtc cgggttcgag ttgagccagg gtgagcggcg gcgctccctg ctccgacagg
     2221 agcgcattca ctgccggcag atatcggttc aactccgcaa tgtcgtcgac ctggtatgcc
     2281 atctccggcg agaagaagtc gacgttgttg tatccgaggc tggtatcgaa agggaactcc
     2341 tgcacgggga gaagctggat cgccgtaacg ccaagatcgc gcaggtacgg aatgcgcgtg
     2401 atgacgtcca gaaaatccgc cacctgctcg cgcttgtcac ggccctgcgc gtcgacgcca
     2461 tagaaggcgc caacgtgcaa ctggtaaata atcaggtcgt tgaaggcggg cgcgtgaaag
     2521 gaggaatcgt gccagggata cgttgtgggg tctctcacca ggcaatcgca atccggaaag
     2581 gccggttgta ttccgagttc gcgggcgtag ggatcgcgtt tgaagccgcc gccggcatcg
     2641 cccaccacgt agaaacggta aggcgcgcct tccttaagac ccgggacgaa gagcgcccaa
     2701 gtgccgtcct cgcggcgcgt caaccgatcg gacaggcgcg gcgtccagcc cggttcacgg
     2761 gacgcggcag cctcggcgcc ggcgactacg tagacatcac gggcgcgcgg cgcccaaaca
     2821 cgaaatgtag ctccgccttc gacgagattg gcgcccatcg gcgtttgcat cgttcggctt
     2881 acagtttgaa gaaaatcttg cgccggtaca gccagtagca gagataccac atcgccagca
     2941 gcaccgcgca ggcttgcgcc accgaggcga agtcgccgag ccattcaaat ttcagcgtga
     3001 acactcctat cgcccggttc agccagccgc gaagcacgat atcgaccgag tagatgaaaa
     3061 tggaattcgc cccgactacg acgagtggaa acgtccacgc acgatattct ttgacttcga
     3121 ccacccagta gaaagccaaa agcatcagga gcacccaccc ggtgctatag agcgtaaagg
     3181 acgtggtgca gattcgcttg atgattggat tccatggatg aatcaccagt ccgaggagaa
     3241 gagacaggaa ggctgcaatt gccagggttc gcatctttac ggcatgcgag ctccgggtca
     3301 tgagcagttt tccggtccat acgccgaaca gcgtggtagc ggtgctggtg atgaaattga
     3361 tattgaccca atacccgccg ttcatgcggc cgaagacgaa tttgtcgata accgcaccga
     3421 tattggtcgt tttcgacata aacggccctt cgctccccgg gaacgccacg aacaatgccc
     3481 agtggccgat cagtatgcct gccgctatca cggcctgcca ccggaagcgc agttgcatga
     3541 tgagatagca aaggaaatac gtaatcccga tctgcgccag cacgttgatg agttggaagt
     3601 gcatctcgcc gcgcgagatc gacattaaaa tctgactcat cagaatgagt cttacgctgc
     3661 gaacggccac gtgccggaag aggtcgcggt tcgtcgctcc gagttccatg cggcgggcaa
     3721 atgcgaaggg catcgcgacg cccaccatga acatgaacgc aggctggatg aggtcccaga
     3781 acgaaatcca ttcccacggc atgtgtctga actggttggc gagggcggtg aaagcgggat
     3841 ttttctcggc gagcgcagcc aggccgaacc cgcccgacac cagcatgatc atgatgaagc
     3901 cgcggtatgc atccagagcg agataacgcg gctgaggtgt ctgtggcggt gtgacggctc
     3961 ccagaggtgc tgcggcggaa accggttgag cagcttgcat cttggtccgg attctagcac
     4021 acgggcaggg aagattgatc aatcagatgg catgctcgtg gaagtgttca tggtctcatc
     4081 gctctgcaca gagaacgacc aagcttcaat ggggccacga gcatgccaga tggcagttta
     4141 tcataagctg gtcttacaga gaactaccaa caaaaaaatc tattgctttt tactttacac
     4201 atccccatac cgagaatcgg ttcgtgatca gcacgaaccg attctcggta gatactcccg
     4261 aagggtggta tccacgtgcg ggtatagtgt accactcctc gggtcgcgaa aagaggccct
     4321 agatgaacgt atatctcgtg gtggctgaag ccacctccag tcgaagtgta tccaccgccg
     4381 tgtctttact cggcggcaaa cctgtgatga gcttggcatg ggtagttcgg tggaagggta
     4441 cggctgattc cctgcgttgt catcttcgga actgttgccc ggaaggttgc acagtag
//
LOCUS       DASTMK010000233         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_12159, whole genome shotgun sequence.
ACCESSION   DASTMK010000233 DASTMK010000000
VERSION     DASTMK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12159"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFQ79_10225"
     CDS             complement(<1..859)
                     /locus_tag="VFQ79_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU0140079.1"
                     /translation="MKALLTSLVCAAFLFATACSGPNAREPAAVSPVVELASVERKDV
                     PVYAEWIGSLDGMVNAAIRAQVTGYLQRQAYNEGAFVRKGQLLFEIDARPFDAALLQA
                     RGQLAQAKGQLAQAQAQLSQAQAQRSKAEADRQRTQLDADRYTSLATGDAVSQQDVDN
                     AVQNNLSAGAQVVAATAQVETAKAQIEAANASVEAAQAAVEAAQINLSFTKLTAPIDG
                     VAGQAQIQVGNLVSPSAGPITTVSTLDPMRVYFAVSEQEYLAFARSADGANPEEAMKR
                     FVLELVLADG"
     gene            1129..1398
                     /locus_tag="VFQ79_10230"
     CDS             1129..1398
                     /locus_tag="VFQ79_10230"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEU0140080.1"
                     /translation="MSNEVLDLRPLPPPQRHGLVFATFNALKLNDSFMLINDHDPNPL
                     RMQMDFMHHGEMGWEYLERGPEAFRIKVTRIAEAKKPKGTAEKSA"
     gene            complement(1632..>2244)
                     /locus_tag="VFQ79_10235"
     CDS             complement(1632..>2244)
                     /locus_tag="VFQ79_10235"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU0140081.1"
                     /translation="QDNPHEDALLTAAAAIRRGEVVAIPTDALYVLVADPYNLHAIGK
                     VFLAKGREIHRSLPLLITDTFMAEDLAKELNSRFYQLSRHFWPGPLTIIVPAGEKVPL
                     KVTGNTGRLALRQVRTPVAMKLVEILQQPIIATSANISGQPTCKTGIEVFGTMDGRLK
                     LVLDGGGCVGTGSTTVDITEPYWRIIKEGAITEKQIAEVLHTM"
BASE COUNT          425 a          651 c          693 g          475 t
ORIGIN      
        1 tgccatccgc gagaaccagc tcgagcacga agcgcttcat cgcctcctct ggattcgcgc
       61 cgtccgcgga ccgtgcgaag gcgagatact cctgctcgct gacggcaaag tacacacgca
      121 tggggtcgag ggtggagacg gtggttatcg gtccggcgct ggggctcacc agattgccga
      181 cctggatctg cgcctggcct gcgacaccgt cgatgggcgc ggtgagtttc gtaaagctaa
      241 gatttatctg agcagcttcc acggccgcct gggcggcttc gacggacgca ttggccgcct
      301 cgatttgcgc cttcgcggtt tccacctggg ctgttgcggc gacgacctga gccccggcac
      361 tcagattgtt ttgcaccgcg ttatccacgt cctgctgaga aaccgcatcg cccgtggcca
      421 gggaggtgta gcgatccgcg tcaagctgcg ttcgctgtcg atccgcttcg gccttcgatc
      481 gctgcgcctg agcctggctt aattgcgcct gggcttgcgc caactgcccc ttcgcctgcg
      541 ccaactggcc acgagcctga agcagcgccg cgtcgaatgg gcgtgcatcg atctcgaaaa
      601 gcaactgacc ctttcggaca aacgcgccct cgttgtaggc ctgccgctgc aggtagccgg
      661 taacctgagc acggatggcc gcgttcacca tgccatcgag cgagccgatc cactccgcgt
      721 aaacgggcac atccttgcgc tctacggatg ccagttcaac cactggcgag acggctgctg
      781 gttcgcgggc attgggcccg gagcacgcgg tagcgaaaag aaacgccgca cagaccaggg
      841 aggttagtag cgccttcatt cggcgctcgc taaacacaac gcgcacatgc ccatccacat
      901 cactttctta acgcttatgg aagttcaagt acattatcca cccgcaccgg gtggcgtgca
      961 aaatcgagta gatcggccca atcgtgaatt cccgtgacgc ccatttcgcg gcatggcctt
     1021 aggcaacggt cgaacaatgt gtggcacgcc tggaaatcct tacaatttgt tcttcaagtg
     1081 tgactctgat cacggactga attggtcggg ttggtataca ctacggacat gtctaacgaa
     1141 gtactcgacc tacggccact tccgccacca cagcggcacg gcctcgtttt cgccactttt
     1201 aacgcattga agctgaatga ttcgtttatg ttgatcaacg atcacgaccc gaatccgctg
     1261 cggatgcaga tggacttcat gcatcatggc gagatggggt gggaatacct ggaacggggg
     1321 ccggaagcgt tccgaatcaa ggtcactcgg attgcagaag cgaagaagcc caaggggacc
     1381 gcggaaaagt ccgcataggg atcccgagac agcctgtgcg ggcggcggct gtcgcccgcg
     1441 atttttggtt ccacctccgc tttgcgcgcg gcttccgtgt cggcaaaccg ggaaaatagc
     1501 cacctcgcct tcgatgtatt tgtaatcggt ttcgtttgct ccccgggcgc acgtgcgctc
     1561 tcccatcgga acgcttccct tctaaactct aacggacccc cgcgatttct tcgctgcatc
     1621 acatacgtgt cttacatggt gtggagtact tcggcgatct gcttttcggt gatagcgcct
     1681 tctttgataa tgcgccagta gggttctgta atatcgacgg tggtgcttcc ggttccgacg
     1741 caaccgccgc catcgaggac gagtttgagg cggccgtcca tggtgccgaa gacctcgatg
     1801 ccggtcttgc aggtaggctg gccggagatg ttggcgctgg tggcgatgat gggctgctgg
     1861 aggatctcga cgagtttcat cgcgaccggg gtccgcacct ggcgcagggc cagacggccg
     1921 gtattgccgg tgactttgag gggcactttc tcaccggcgg gaacgataat ggtgagcggg
     1981 ccgggccaga agtggcggga gagctgatag aagcgggagt tcagttcttt agccaggtct
     2041 tccgccataa aggtgtcggt gattagtagg ggcagcgagc ggtggatctc ccgccctttg
     2101 gcaagaaaca ctttgccgat ggcgtggaga ttgtaaggat cagccacgag gacgtagagc
     2161 gcgtcggttg gaatggccac gacttccccg cggcgaatgg cggcggcggc ggtgagaagt
     2221 gcgtcctcat gagggttgtc ctgg
//
LOCUS       DASTMK010000234        16629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_587177, whole genome shotgun sequence.
ACCESSION   DASTMK010000234 DASTMK010000000
VERSION     DASTMK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16629
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587177"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFQ79_10240"
     CDS             complement(<1..352)
                     /locus_tag="VFQ79_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140082.1"
                     /translation="MPRDSRRVRALACPFFLLAAYGISGTGQPSWEQCMAEMERFERE
                     SDYAQVEKALLAALNQYGQLAPQDPCLAVTLYKLGDFYRVLGRTREAERFYLRALSAG
                     EKSAVANHSGLLNTL"
     gene            complement(361..924)
                     /locus_tag="VFQ79_10245"
     CDS             complement(361..924)
                     /locus_tag="VFQ79_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140083.1"
                     /translation="MNKLLYIAVGTLAVAGFGRAEEKCQTIRGVLQAYLDPEVRFWVN
                     EVHGVLDDTRMVVGKMTALAPATTSIDRGIGIDRDGLQMWDFGEDGALTVELPVGLHL
                     RPAPGPQGFGVYRGMGRIVDGTGPFANATGTVLYEGSWVLWFLTEGDSPTPQGRWNAD
                     VTMRICKGKTRQGPAGVRQSGESPEQK"
     gene            1059..2855
                     /locus_tag="VFQ79_10250"
     CDS             1059..2855
                     /locus_tag="VFQ79_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0140084.1"
                     /translation="MQFSVGAWRVRPELNSLIQDGREIHITPKAMDVLVYLAQRPGQV
                     VPKEAILQDLWAGLSVTDDALTRCIGEIRRAVNDTARQPAFIETVAKRGYRILLPVTC
                     DPANGSGAPASQKWRWIAPAAGFALLAILLVVRMVWYGRAGASEIQSLAVLPLANLTG
                     DPGQDYFADGMTEELITMLAHHGCNVISRTSVMRYKGTRQPLQQIARDLNVGGVIEGS
                     VMRSGGRVRITAQLIDVTTDLHRWSGTFEREINDVLTLQSEIALAVIAEIRPLLKPGE
                     FARNKPPRRVVPEAYEDYLKGLHFFRLSRWNEAAGYFEQATQKDPGDALSFALLCEAD
                     RMVSFIADLPKSDRALNAMHRALELDNTLAEAVINIGDFKFYGQWDWAAGEAEFRRAI
                     ELDPRSLDAAHHYLGCQHVLARWETAFAECKRAMQLDPLSKRTNEALLGLLVNTHQWE
                     VALQQFRKTTELYPDSLSAWRYAGYVHAYLGNDREAVAAHLRADQFAGRTPEQISALE
                     QAAGVAGLRGYWRERLRQLERKAKQTRVPPLDFAMISVHLGDHDAAFRFLDAAYREHS
                     PRLAWLKARAIWDPLRSDPRFQALVLKMGFPK"
     gene            complement(2883..3563)
                     /locus_tag="VFQ79_10255"
     CDS             complement(2883..3563)
                     /locus_tag="VFQ79_10255"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0140085.1"
                     /translation="MTASEVDTLEQLADEDVVRKILEGEIELFEILMRRHNRRLFRAA
                     RSILRNDWEAEDVVQDAYVRAYTHLASFEGRAKFSTWLTRIAVYEALARKRRDSRMDE
                     IEVEVLAAGDPHDLSDRVNRNPEQQVMDEQLRTSIEKAIDQLPLHYRTVFVLRDVQGL
                     DTAETAECLGLGEQAVKTRLHRARNILKDVLAVQTGATVEHAFNFLGERCDRMVARVM
                     ARISGHRG"
     gene            3680..4120
                     /locus_tag="VFQ79_10260"
     CDS             3680..4120
                     /locus_tag="VFQ79_10260"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0140086.1"
                     /translation="MTQVNSHDRAWKTLRLVYGLVPLLAGLDKFFNILTNWEQYLSPL
                     AAQFLPVPASVFMGFVGIVEMVAGIAVLTRWTKPAAYVVAVWLAAIALNLVSSGAYLD
                     IAVRDLVMAAGAYSLAELTAARETGSLRSRHPASRTAEVQSGAA"
     gene            4148..5464
                     /locus_tag="VFQ79_10265"
     CDS             4148..5464
                     /locus_tag="VFQ79_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140087.1"
                     /translation="MRHWKWTRNLLVSVLAGIPVAAVAFTLFFTNRGVQAQSDSRDPA
                     LRNGETLIEQGRKIFRYDTFGDQAFWGGTLRLHEAIRQVSPRAALSAGLKVDSESLPE
                     SVLAAIRDGRINIDDPGVTQTLLKLNAVVGVTGYFDESGILNSMGIQCALCHSTVDNS
                     VAPGIGRRRDGWPNRDLDVGTIVSLAPDLSFFTKLLQVDDATVRKVLRSWGPGKFDAQ
                     LAFDGKAFRADGKSAATLIPPAFGMGGVNLHTWTGWGSVTHWNALVAVLEMHGQGTFY
                     DPRLNDEERFPVAARAGLGNVRSNPDLVTPKLAALQMYQLALPVPKRGEFNPFNFAAI
                     RGKQIFETKAQCAQCHVPPIYTEPGWNMHTPEEICTDDFQASRSPDGAYRTTPLGGLF
                     TREKGGFYHDGRFKTLSEVVDHYNACKDLGLSGSEHSDLVEYLKTF"
     gene            complement(5469..6362)
                     /locus_tag="VFQ79_10270"
     CDS             complement(5469..6362)
                     /locus_tag="VFQ79_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0140088.1"
                     /translation="MTRRQLAVLAVPAAVARPATARVKKGICSIIFPKGMPAADRFRE
                     TRNAGFEGIEIRLGEEVTLSSTPDEVKRVGDEARKAGVAISAMWVSQGLSDNPLNHAS
                     PQMRQAGVTAIDTAIDFAAYMNCPALLLVPGRLGNGPKFITGYQDTWTRVTAELKKLV
                     PHAAKAKVILGPENVWNKFLVSPLEMRSFVDQFKSPWVQAFFDAGNVMQFGYPQDWIL
                     TLGPRMKNVHLKDYKLSARSEQGRFVNLFEGDVDWKEVMAAIVKVGYQGFVAPEYSYD
                     ANDPDRIRKLSALTDKILAMA"
     gene            6439..7710
                     /locus_tag="VFQ79_10275"
     CDS             6439..7710
                     /locus_tag="VFQ79_10275"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEU0140089.1"
                     /translation="MQRRSFLNTVMAVPAAAAQMTASTNPKIEKLRAAAMEVLKPSKK
                     SLEHGLELHANSMVVESYGFSPRSGYDADLYRREFEAGASDQELEDLREEMLMTRCVT
                     DARDREEYKQAWDASGVTCVFQNAGEEGQDPMRLIKRLARFTYVTDTLGDFVTRAAFP
                     DDIVNAKKQNKHALYFSGNGVPVTGQWTSVQDELRYIRIFYQLGIRMMHLTYNRRNML
                     GDGCAEPANGGISDFGKAAIAEMNRIGVIVDVAHSGWSTSLEAAKASAKPMVASHSTA
                     AEINRHIRAKPDEVIRAIADTGGYIGICCIPSFLGRSGDINAMLDHIDYVVKRFGADH
                     VAIGTDVAYTPSSSDGLKLPGRRSRPRFENFWPPDAFANYPSQHASMAWTNWPFFTVG
                     MVERGHSDQDIQKILGGNVLRVARAVLPGTR"
     gene            7776..9923
                     /locus_tag="VFQ79_10280"
     CDS             7776..9923
                     /locus_tag="VFQ79_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEU0140090.1"
                     /translation="MASVQKQINDTPFRIAVSASFTAEPLLRPLMFWSGQLNREFDVR
                     FAPYNQILQTLLDRGGEFASNTHGVNVALVRVEDLGHLPDGGFDLERIEANVRQLLDA
                     VRSAPEQGGTPLVVILSPASDAFLEEPIRRAFSDRMDKLISQSLHEATGVQCIDHHQI
                     QALYPVDRRTAGEGERLGRIPYTEMYFCVLATIIVRFARALLAPPYKVIVTDCDNTLW
                     KGICGEDGPAGVVLDPPRRALHEFLLEQHEAGALLAMASKNNEQDVFDTFAEHPEFPL
                     QLRHFTAWRLNWESKVENLLSLADELSLGLDSFVFLDDSPKECAEVEEGVPEALTLVL
                     PEPVENTPHFLRHVWAFDRPVVTEEDRQRSVYYRQQERFGREVRQAADLEHFMATLQL
                     DVRFLPLTPERLARVAQLTQRTNQFNFTTIRRTESGISTLVLNEGFECLTIDVSDRFG
                     EYGLVGIVLFREGGDALEIDTFLLSCRVLGRGVEHRVMARLGEIAAGRGLAAVAGELR
                     FTAKNLPARQFLESLGRFEPIDDGLLYRIPAEQASGVRWRPATPEGTAPKRDRKAHGR
                     PLESIDFGRIARELSTPEQIREAVRRSRVSQSGFLEMSDTERQLAQIWSDLLERDTVR
                     REDNFFDLGGHSLLAVLLILRVKEAFGIELPIDDVYTSGLTLADLAARIELHRETAGM
                     DPAEYAKLLDDIEHLSDEEVRELLARGDPGSFT"
     gene            9927..11129
                     /locus_tag="VFQ79_10285"
     CDS             9927..11129
                     /locus_tag="VFQ79_10285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0140091.1"
                     /translation="MRILLAANATYLPPRGGSTRSNLVWLHALARAGHECRIVSPAPD
                     PSAGDDRVEKDGLTIVRIAHLSRHARVVRDEIEVFQPDWVLVSSEDLSRVLLREAHHA
                     APGSLIYLAHTPQFFPFGPESWNPDSEATEIIRSARAVVAIGRHVAGYIKQHAGVRAT
                     VIHPPIYGSPPFPRYGRFGSGAILTINPCDVKGIAIFLAVAERCPEYRFEGLTGWGTT
                     RRDLERMSRVPNVSALQPVADIGEALSRASLLLMPSLWYEGFGLIAMEAMLGGLPVIS
                     SNSGGLVEAKRGTGYVIPVRPIEQYKSEFDENHMPRPVLPEQDIEPWENAVRTLMSDQ
                     SAWEREAERSRTAAEEFASGLRVEEFEKLLLSLKPAGPTPRAKVRVENLTPAQRALLF
                     ERLRRRKS"
     gene            11129..13117
                     /locus_tag="VFQ79_10290"
     CDS             11129..13117
                     /locus_tag="VFQ79_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0140092.1"
                     /translation="MRVLLAQNSLYYPSHGGGDKSNRLLMEALAARGHDVRVVARVEQ
                     FGREAHEKYLDDLRSRGASGTVTTEGVHLRLNGVDVLTVTLNPQFRAVFAERVTAFDP
                     DVIVTSTDDPAQLLFEIAVRSPRARVVHLVRATIAVPFGPDSSSPSVARTGMLRQADA
                     VVGVSEYVARYVREHGKMDAVHVPISLMDGGEPTMMGNPGNRFVTLVNPCAVKGISIF
                     TALANEMPEVAFAAVPTWGTNALDYAELRRHANITIIPPSDNIDEILRQTRVLLVPSL
                     WAEARSRIVVEAMARGVPVIASDIGGIPEAKLGVEYLIPVNPISTYEPAVDEHMVPVA
                     KVPPQNIAPWREALERLIRDGAHYLDVSLRSREAALSYIASLSVEPFERILDRVIAAP
                     KRGAATAKPPALSPERQRLLALRLRQKSAVSGWFAGPTKGKPVLLCFPHAGAGTVHYR
                     GWQEALPEVALCPIRLPGRETRMQEPPIDEMRALVKALAPEIEQHVQATFAFFGHSMG
                     AGIAFEMARELRRRGAAMPVALFVSAARAPQWRLGYQPPPEPEDAELVEQLARLGSMS
                     PEVLRLALPVLRADTRLYRNYVYQPEDPLPVSLCAYGGEQDDSVPRQDIEAWREQAGG
                     TFRMRIFPGGHFYLQENPAAFLSALHADLDQAGVVGPR"
     gene            complement(13090..14409)
                     /locus_tag="VFQ79_10295"
     CDS             complement(13090..14409)
                     /locus_tag="VFQ79_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0140093.1"
                     /translation="MLTASRQPGTPFWTLLLGNRNYRYTWLGQIVSEVGDHFNTIAVL
                     SLALHTTGSGAAVGGVMLARTLGGIAAGPIAGVVLDRMDRKQVMIVSDLFRAVAALAF
                     VLVITHKQEWLLYFLSASLMFASPFFTSGRSAILPDITRPEELHTANALTLTTAWLTL
                     SIGAFLGGASTMRFGYEWAFVVNAFSFLFSAGAISRLRPSGAGFRAATDSGAPARSVR
                     AFWDDFRSGMAYIRSTPLVLGIALVLVGWASGGGAAQILFTLYGELVFKLGPSGVGFI
                     WGSAGIGLVAGGFLAHWLGPHLDFVRYKHAIAILLFVHGAAYVLFAVSPDVWWAVLFI
                     ALSRIGMGANNILNRTMLLHHVPNRFRGRVFSAMEMILNGAMMISMTAASVAAVRYPV
                     RGIGVIAGVLSTSTALFWAAANYAGKLPEPPAVPEQETDQRGPTTPA"
     gene            complement(14490..15101)
                     /locus_tag="VFQ79_10300"
     CDS             complement(14490..15101)
                     /locus_tag="VFQ79_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0140094.1"
                     /translation="MEDSSLVHSRDENEVSALMFSVYMMRPDVTQLFLDNGAEPAFHE
                     AAALNLTDRLSAFLAQSPALLNAPAKDGFPALTLACFFGAMDAVDLLLRSGADPNIPA
                     QNKMRVTALHSAVANRDLAKAERLVRMLLAHHANPNVKQEGSLTPLHHPAALGHKDLA
                     RILLEHGADPNAAADNGKIPIDLARENGHADLVELLSNWQASK"
     gene            complement(15148..>16629)
                     /locus_tag="VFQ79_10305"
     CDS             complement(15148..>16629)
                     /locus_tag="VFQ79_10305"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140095.1"
                     /translation="KADAVARAEVQFAEGSYEDALSSFLSAASLDPVDLTAQTNAAVC
                     LERLGRWTEAVELLQRTLESDPASPDALMGLAICYLHLEKPGKALEALEPLYAAAPVS
                     GRARFARAVALQLVSRFEEAIEEYKEILTSEPDSAEVLTNLVAIGIATAQEPLTREYS
                     ERLISLQPESTPAIRGLAACALWRQDYEEASRHCGSLVELVPEDANVWLNLGISLLRS
                     YHPLEAAKAFLEAMRLRCDKTQAAAVIAPALRQCGALEEARACCEQGLSEQPENMPLA
                     MILAGVLEEAGDAEAAAKQYTKVLMVQPENTEAWFRLGCLRLAQEDPRTAFEAFQSAV
                     RLRPEWLEALLNLALSSWRAGDLEDARQYYGEAAKLHPGSLEAVRGLAGVAIELGRYD
                     EALDLHGRLIDLGDSSPELYFNTGLLLDKTGQREDAVRLYKEALALNSQFSEALLNLG
                     HVLKLLGREEEARAAWLQAAHSDGHSGDSVQSLRSGAVFGRHS"
BASE COUNT         3281 a         4923 c         5136 g         3289 t
ORIGIN      
        1 tcagggtgtt cagcaaaccg gaatggtttg ccacggcgct cttttccccc gccgatagcg
       61 cccgcaggta gaaccgctcc gcttccctgg ttctgcccag aaccctgtag aagtcgccca
      121 atttatagag tgtgacggcc agacagggat cctgcggagc gagctggccg tattggttca
      181 aagcggccaa tagagccttc tcgacttggg catagtcgct ttcccgctcg aaacgctcca
      241 tttccgccat acactgctcc caggacggtt gcccggtacc ggagattcca tacgcggcca
      301 gcaggaagaa cgggcatgct aaagcccgca cgcggcggga atccctcggc atggttcacc
      361 tcacttttgc tccggcgact cgccggactg gcggacacct gctggtcctt gcctggtttt
      421 gcctttgcag atcctcatgg tcacatccgc gttccatcgt ccttgaggag ttggcgaatc
      481 accctcggtc aaaaaccaca gaacccagga gccttcgtat aggaccgtgc cggtcgcatt
      541 ggcgaacgga cccgttccat cgacgatcct gcccattccc cggtaaacgc cgaagccctg
      601 tgggcccgga gcgggccgaa gatggagacc gacgggcaat tcgaccgtga gcgccccgtc
      661 ctcgccgaaa tcccacatct ggaggccatc gcgatcaatc ccaattcctc tgtcaatcga
      721 cgtcgtggcc ggggcgagcg ccgtcatctt ccccaccacc atcctggtgt cgtccagaac
      781 gccgtgaacc tcattcaccc aaaaacgtac ctccggatcg agatacgcct gaagcacgcc
      841 ccggattgtc tggcatttct cctcggcgcg gccgaagccg gcaaccgcga gggtcccaac
      901 tgcgatgtaa agtagtttgt tcatttctat acctccttgc aggtcaattg tcggccggag
      961 gcgggcgatg cgtcccgaca aacgcccgat atttctccga caatttgcag ggtttctatc
     1021 gggcgaagcg ggtgatagca tatcagcgga ggcgagcggt gcagttcagc gtgggggcat
     1081 ggcgggtccg gccggagctg aacagcctta tccaggacgg ccgtgagatc catatcactc
     1141 cgaaagccat ggacgtcctc gtctacttgg cccaacgtcc tggccaagtg gttcccaagg
     1201 aggcaattct tcaggatctc tgggccggat tatccgtaac cgacgatgcg ctcacccgct
     1261 gcatcgggga gatccgccgc gctgtgaatg acacggcccg ccagccggca ttcatcgaga
     1321 cggtggccaa acggggatac cggatacttc tcccggtcac ctgcgacccg gccaacggca
     1381 gcggggcgcc ggcgtcgcag aagtggcgtt ggattgctcc ggccgccggg ttcgctctac
     1441 tggccatcct tctggttgtg cgcatggtct ggtacggccg tgcaggtgca tcggagatcc
     1501 agtcactggc cgttctgccc ctggccaatc tgaccggcga ccccggtcag gattacttcg
     1561 ccgacggcat gacggaagag ctgatcacaa tgctggcgca tcacggctgc aatgtaattt
     1621 cacgtacatc ggtgatgcgc tacaaaggaa cgcgccaacc cttgcagcag attgcgcgcg
     1681 acctcaacgt gggcggtgtg atcgaaggca gtgtcatgcg gtccggaggg cgggtgcgca
     1741 ttaccgcgca gttgatcgac gttactaccg atctgcatcg ctggtccgga acgttcgagc
     1801 gcgaaatcaa cgacgttctc acgcttcaga gtgaaattgc gctggccgtc atcgcggaga
     1861 tcaggccgct gctaaagccg ggcgagttcg cccgaaacaa gccgccgcgc agagttgttc
     1921 ccgaggcgta tgaagattac ctcaaggggc tccatttttt ccgcctgagc cgctggaacg
     1981 aagccgctgg gtatttcgaa caggccaccc aaaaagaccc gggcgatgcc ttgtcgtttg
     2041 cgctcctgtg cgaagcggac agaatggtga gcttcatcgc cgatcttccg aaaagcgacc
     2101 gggcactgaa cgcaatgcac agagcgctgg agttggacaa caccctggca gaggcagtca
     2161 ttaacatagg agatttcaag ttctatggcc agtgggactg ggcagccgga gaagcggaat
     2221 tccggcgggc catagagctt gatccccgct cgctcgatgc ggcgcatcat taccttggtt
     2281 gccaacacgt gctggcacgg tgggagacag cctttgccga gtgcaaacgt gctatgcaac
     2341 tcgatccgtt atccaagagg acgaacgagg cactgcttgg gctgctcgtg aacacacacc
     2401 agtgggaggt ggcccttcag cagttccgga agactaccga gttgtatccg gattccctct
     2461 ctgcttggcg gtacgcaggc tacgtgcatg cgtacctcgg aaacgaccgg gaggcggtgg
     2521 ccgctcacct tcgggcggat cagttcgccg gtaggacccc ggagcagata agcgcgttag
     2581 agcaggccgc cggcgtcgcc gggctccgcg gttattggcg ggagcgcctg cggcagttgg
     2641 agcgcaaggc gaagcagacc agggttccgc cgctcgattt cgcgatgatc tccgtgcatc
     2701 tgggcgatca cgatgccgcc ttcaggttcc tcgatgcggc ataccgggag cactctccgc
     2761 ggcttgcctg gctgaaagcc cgggccatat gggacccgct gcggtccgat ccacgctttc
     2821 aagcgctggt cctgaaaatg ggcttcccga aatgagccgg tagccggctt cgacaagaca
     2881 gactagccgc ggtgaccgga aatccgcgcc ataacgcgag ccaccatgcg gtcgcagcgt
     2941 tcaccgagga agttgaacgc atgctccaca gttgcgccgg tttgaactgc gaggacgtct
     3001 ttaagaatat ttcgcgcgcg gtgcaatcgt gttttcacgg cctgttcccc gagtcccagg
     3061 cattctgccg tttcagccgt atcgagaccc tgcacatccc gaaggacgaa aactgtccgg
     3121 tagtgcaggg gtagttgatc gatggctttc tcgatggatg ttcgcaactg ttcatccatc
     3181 acctgctgtt cggggtttcg attcactcgg tccgagagat catgcggatc tccagcagct
     3241 aacacctcga cctctatctc gtccatccgg ctgtcccgcc gttttctcgc gagagcttcg
     3301 tagaccgcaa tgcgggtaag ccatgtgctg aacttcgccc tcccttcgaa gctggcgaga
     3361 tgtgtatacg ctcgaacata ggcatcctgg acgacatctt cggcttccca gtcgttccgc
     3421 aggatcgagc gcgccgcacg aaacagccgg cggttgtgcc ttcgcatcaa aatctcgaaa
     3481 agctcgatct caccctccag aatcttgcga acaacgtcct cgtccgccaa ttgctccagc
     3541 gtgtctacct cacttgcggt catctactca tacagagtcc caatggtcct cttcgtttca
     3601 agttttcgaa gatttcacac ctgttgcctt ctggaacctt tccggaaccg gggactcggt
     3661 tgaagtggag ggcaggaaaa tgacgcaggt aaattctcac gaccgggctt ggaaaacact
     3721 gaggctcgtc tatggtctcg ttccgctgct ggcaggtctc gataagtttt tcaacattct
     3781 caccaactgg gagcagtacc tcagcccctt ggcggcgcag tttctccccg ttccggcatc
     3841 ggtgttcatg ggctttgtgg gcattgtgga aatggttgcc ggcatagcgg tgctcacccg
     3901 ttggacaaag ccggcggcat acgtagtcgc ggtctggctt gccgcgattg ctctgaatct
     3961 ggtcagttcg ggcgcctacc tggatatcgc cgtacgtgac ttggtgatgg ccgccggcgc
     4021 gtactccctt gcggaattaa ctgccgcacg cgagaccggc agcctgcgaa gccgtcaccc
     4081 ggcgtcccga accgccgaag ttcagtccgg cgcggcataa ctctttcgcg aggagaggag
     4141 attcgaaatg cggcattgga aatggaccag gaatcttctg gtttcggtgt tagccggtat
     4201 cccggtggcg gcggttgcat tcacgttgtt cttcaccaac cgcggggtgc aagcacagtc
     4261 cgactcgagg gatccggcgc tccgaaatgg ggaaacactc attgagcaag gccggaagat
     4321 cttccgctac gacacttttg gagaccaggc gttctgggga ggcacgctga gactacacga
     4381 ggcgatccgg caggtgagtc cgcgtgcggc cctttccgcc gggttgaaag tggactccga
     4441 gagcctgccg gagagcgtgc tcgcagcgat tcgggatggc aggatcaaca tcgacgatcc
     4501 cggtgtgacc cagactttgc tgaagcttaa cgcggtggtc ggtgtcaccg gatacttcga
     4561 tgagagcggc attctgaact ctatgggaat ccagtgtgcc ctgtgccact ctacggtgga
     4621 caactccgtg gcgcctggaa tcggacggcg ccgggacggc tggccgaacc gcgatctcga
     4681 tgtgggaaca atcgtctcgt tggcgcctga tctgagcttc tttacaaagc tgcttcaagt
     4741 ggatgatgcc acggtgcgga aggtccttcg aagttggggc cctggcaagt tcgatgccca
     4801 gcttgcgttc gacgggaaag cgtttcgagc ggatggaaag tcagccgcga cattgattcc
     4861 gccggcgttc ggaatgggcg gtgtgaattt gcatacctgg accggctggg gctcggtgac
     4921 gcattggaat gccctggtgg cggtgttgga gatgcacggg caaggtacct tctacgatcc
     4981 tcgcctcaat gacgaggagc ggtttcctgt tgcggcgcgg gcaggattgg ggaacgtccg
     5041 aagcaatccg gatctggtga ccccgaagct tgccgcactc cagatgtatc aactggcgct
     5101 tccggttccg aagcgtggcg agttcaaccc gttcaacttc gctgcgatcc gtggcaagca
     5161 gatttttgaa accaaagctc agtgcgcgca atgccacgta ccacccattt acaccgagcc
     5221 cggctggaac atgcataccc cggaggagat ctgcacggac gattttcagg caagccggtc
     5281 gccggacggc gcataccgca ctacgcccct cggtggactc ttcacacggg aaaagggcgg
     5341 cttttaccac gacgggcgat tcaagactct cagcgaagta gtcgaccact acaacgcctg
     5401 taaggacctc ggtttatcgg gcagcgaaca cagcgatctg gtcgagtatc tgaagacgtt
     5461 ttgacgaatc aggccatcgc caggatcttg tcggtgagtg cggagagctt cctgatccgg
     5521 tcgggatcgt tggcgtcgta ggaatactcg ggtgcgacga agccctggta gccgaccttg
     5581 acgatggccg ccataacctc tttccaatcc acgtcgcctt cgaacaggtt gacgaagcgg
     5641 ccctgctcgg agcgggcaga gagcttgtaa tccttgagat gaacgttttt catgcgaggt
     5701 cccagcgtga ggatccaatc ctgcggatag ccgaactgca tgacgttgcc ggcatcgaag
     5761 aacgcctgta cccatgggct cttgaactga tcaacgaacg aacgcatctc gagcgggctt
     5821 accaggaatt tgttccagac gttctcgggt cccaggatga ccttcgcttt cgccgcatgc
     5881 ggcaccagtt tcttaagttc ggctgtcaca cgtgtccacg tatcctgata gccggtgatg
     5941 aacttcgggc catttcctaa acggcccgga accagcagca gtgccgggca gttcatataa
     6001 gcggcgaaat cgatggcggt gtcgattgcc gtaacccccg cttggcgcat ctgtggcgag
     6061 gcgtggttta acggattgtc gctcaagcct tgtgagaccc acatggcgct gatggcgaca
     6121 cctgctttcc gggcctcgtc cccgacccgt ttcacttcat cgggagtgga ggaaagagtg
     6181 acctcttcac ccagccggat ctcgattcct tcgaagccgg cattgcgcgt ttcgcgaaaa
     6241 cggtctgccg ccggcatacc tttaggaaaa atgatggaac agatgccttt cttcacgcga
     6301 gccgtagcag gacgggcaac cgcggcaggg acagcaagaa cggcgagttg acggcgagtc
     6361 ataagcgttt tcaatcgtat caccacggtg ccgcaacaag cgcttcatgc gtttcacgta
     6421 taatgcggca gagccaatat gcagagacgc agcttcctga acacagtgat ggcggtaccg
     6481 gcggccgccg cccagatgac ggcgagtacg aatccgaaga tcgagaaatt gcgcgcagcc
     6541 gccatggagg tcctgaagcc gagcaagaag agcctggagc acgggctcga actgcacgcc
     6601 aattcgatgg tagtggaatc gtacgggttc tcaccccgtt ccggttacga cgccgatcta
     6661 taccgtcgcg agttcgaagc cggcgcgtca gaccaggaac tggaggacct tcgcgaagag
     6721 atgttgatga cgcgatgcgt caccgacgcg cgcgaccgcg aggagtacaa acaggcctgg
     6781 gatgcctccg gcgtgacgtg cgtttttcag aacgcgggcg aagagggcca ggacccgatg
     6841 cgcctgatca agcgcctggc gcgtttcacg tacgtgaccg acacgctggg cgatttcgtt
     6901 acacgggccg ccttcccgga cgacatcgtg aacgccaaaa aacaaaacaa acacgctttg
     6961 tatttttccg ggaacggcgt accggttacg ggtcaatgga cttcggttca ggacgaattg
     7021 cgctacatac gcatctttta tcagctcggc atacggatga tgcacctgac atacaaccgg
     7081 cggaacatgc tcggggacgg ttgtgcggag cccgccaatg gaggaatcag cgatttcggc
     7141 aaagccgcca ttgcggagat gaaccgtata ggcgtcatcg tcgacgtggc gcattcggga
     7201 tggagcacga gtctggaagc tgcgaaagcg tcggcgaaac cgatggtggc gagccattcc
     7261 acggcggcgg agatcaaccg gcatatccgc gccaagccgg atgaagtgat tcgtgccatc
     7321 gcggacaccg gcgggtacat cggcatttgt tgtatcccga gttttctggg gcggtccggc
     7381 gatatcaacg cgatgctcga ccacatcgac tatgtggtca agcgcttcgg agcggaccat
     7441 gtcgcgatcg gcacggatgt tgcctatacg ccgtcgagca gcgatggatt gaagctgccc
     7501 ggacgccgct cccggccccg gtttgagaat ttctggccgc ccgatgcgtt tgccaactat
     7561 ccctcgcagc acgccagcat ggcatggaca aactggccgt tcttcacagt aggcatggtg
     7621 gagcggggcc attccgacca ggacatccag aagatcctgg gaggaaacgt ccttcgcgtc
     7681 gcacgcgccg tactgcccgg taccaggtag ctcacaggag cggtcgcatt cttggcgaaa
     7741 cgtgttaccg cccggttatt ctgaaataga gaactgtggc cagcgttcaa aaacaaatca
     7801 atgacacgcc gttccgcata gccgtcagcg ctagttttac cgcggagccc cttttacgcc
     7861 cgttgatgtt ttggagcggg cagctcaatc gcgagttcga cgttcgattc gccccctaca
     7921 atcagatcct gcaaacgctg ctcgaccgcg gcggcgaatt cgcttccaat acgcacgggg
     7981 taaatgtggc cctggtacgg gtggaggatc tcggccatct ccccgacgga ggcttcgatc
     8041 tggaacggat tgaggcgaac gtgcggcagt tgctggatgc cgtccgctcc gcaccggagc
     8101 aaggtgggac gccgctggtg gttatactgt cccccgcgtc ggatgcgttt ctggaggaac
     8161 ccatccggcg tgcgttttca gaccggatgg acaaactgat ctcgcagtcg ctccacgagg
     8221 cgaccggcgt ccagtgtatc gatcatcatc agatccaggc gctgtatccg gtcgatcgca
     8281 gaacggccgg ggaaggggaa cgcctcggac gcatccccta cacggagatg tatttctgcg
     8341 tgctggccac gatcatcgtg cggttcgcgc gcgctctgct cgcgccgccc tacaaagtca
     8401 tcgtcacgga ttgcgacaac acgttgtgga agggtatttg cggcgaggac ggccccgcgg
     8461 gcgtggtgct ggacccgccc cgccgggcgc tgcacgaatt tctgctggag cagcacgaag
     8521 ccggcgcgct gctggcgatg gcgagcaaga ataacgaaca ggacgttttc gatacgttcg
     8581 ccgaacatcc ggagtttccg cttcagctca ggcatttcac cgcctggcgt ctgaactggg
     8641 agtcgaaagt agagaacctg ttgtcactgg ccgacgagct ttcgttgggt ctggacagct
     8701 tcgtgtttct cgacgacagc cccaaggaat gcgccgaggt ggaagagggt gtacccgaag
     8761 ctctgacgct tgtactgccg gaaccggtgg agaacacgcc ccattttctc cgtcatgtct
     8821 gggcgtttga ccgcccggtg gtgacggagg aagatcgcca acgcagcgtc tattaccgcc
     8881 agcaggagcg ttttggccgc gaggtccgcc aagcggccga tctcgagcat tttatggcca
     8941 cgctccaact cgacgtacgc ttcctgccgc ttactcccga aagactggcg cgtgtggcac
     9001 aactgacgca acgcacgaac cagttcaact tcacgaccat ccggcgcaca gaatccggca
     9061 tctcgactct ggtgctgaat gaaggcttcg aatgtctcac catcgatgtc tccgaccggt
     9121 tcggcgagta cggtctggta ggaatcgttc tatttcgtga aggtggagac gcgctggaga
     9181 tcgacacgtt tctgctgagc tgccgcgtac tgggccgtgg cgtcgagcat cgtgtgatgg
     9241 cgcggctcgg agaaatagcg gctggaagag gactggccgc ggtggcgggt gagctacggt
     9301 ttacagcgaa gaatctcccc gcacgccagt tcctggagag cctcgggcgg tttgaaccga
     9361 tcgacgacgg gctgctatat cgcatccccg ccgaacaggc gagcggagtg cgctggcgtc
     9421 cggccacacc ggaaggaact gctccgaagc gcgaccggaa ggcgcacggc cgtccgcttg
     9481 agtcgatcga cttcggccgc atcgcgcgcg agttgtcgac gcccgaacaa atccgcgaag
     9541 cggtacgccg ttcacgtgtg agccagtccg ggtttctcga aatgagcgat accgaacgtc
     9601 aactggcgca gatctggtca gacctgctgg agcgtgatac cgtacgccgc gaagacaact
     9661 ttttcgattt ggggggacat tcgttactgg ccgtactgct gattcttcgc gtgaaagagg
     9721 cgttcgggat tgagctgccg atcgatgatg tgtacacttc cgggttgacg ctggcggacc
     9781 tggcggcccg gattgagttg catcgggaaa cggcaggcat ggaccccgcg gaatacgcca
     9841 aactgctcga tgatatcgaa cacctctcgg atgaggaagt gcgcgagctt ctggcgcgcg
     9901 gggatccggg ttccttcact taggtcatgc gaatactgct cgccgccaac gctacgtatc
     9961 tgccgccacg cggtggatcc acgcgtagca acctcgtctg gctgcacgcc ctcgcccgcg
    10021 ccggccatga gtgtagaatc gtctctcctg cgcccgatcc atcggctggc gacgaccgtg
    10081 tggagaaaga cgggctgacc attgtccgga ttgctcattt atcgcggcac gcgagggtgg
    10141 tccgcgacga gatcgaggtc tttcaacctg attgggttct ggtttcttcc gaggatctga
    10201 gccgtgtatt gttgcgcgag gcccatcacg cggcgcccgg cagcctgatt tacctggcac
    10261 acacaccgca gttctttccg ttcgggccgg agagctggaa tccggattcc gaagcaacgg
    10321 aaatcatacg gagtgcgcgc gccgtggttg ccatcggccg gcatgtagcc ggctatatca
    10381 agcagcatgc cggtgtgcgg gccaccgtga tccacccgcc catttacgga tcgccgccgt
    10441 ttccccgcta tggccggttc gggtccggcg cgatcctgac gatcaatccc tgcgatgtaa
    10501 aaggtatcgc gatttttctg gcggtggccg aacgctgtcc ggagtatcga tttgaggggc
    10561 tcaccggctg gggtacaaca cgccgtgacc tggagcgcat gtcgagggtg ccgaacgttt
    10621 ccgccctgca accggtggcg gatatcggcg aggcgctctc gcgggcatcg ctgctactga
    10681 tgccgtcgct gtggtacgaa ggattcgggc tgattgccat ggaagcgatg ctcggaggcc
    10741 ttccggtgat ttcgagcaat tcgggcggat tggtggaagc gaaacgcgga acgggatacg
    10801 tgattccggt gcggccgatt gagcaataca aatccgagtt cgatgagaat catatgccgc
    10861 ggccggttct gccggagcag gatatcgagc cttgggaaaa cgctgtccgc acactgatga
    10921 gcgatcagag cgcttgggaa cgcgaggcgg agcggtcacg caccgcagcc gaggagtttg
    10981 catccgggtt gcgcgtggag gaattcgaaa aattgttgct ttcgctcaag ccggccggac
    11041 caacaccgcg ggctaaagtg cgagtcgaga acctgactcc cgcacagcgt gccctgttgt
    11101 tcgaacgcct gcggcgtcga aagtcgtgat gcgggtcctg ctggcacaga attcgctgta
    11161 ttacccctcg cacggcggcg gcgacaaatc gaaccggctg ctgatggagg cgctggcggc
    11221 gcgcgggcat gatgtgcgcg tagtggctcg ggtggagcaa ttcgggcgcg aggcgcatga
    11281 aaaatacctc gacgatctgc gctcccgcgg cgcgtccggc accgtcacaa cagagggcgt
    11341 gcatctgcgc ctgaacggcg tagatgtgct cactgttacc ttgaatcctc agttccgcgc
    11401 cgtgttcgcc gaacgggtga cggccttcga cccggatgtc atcgtaacct ccacggacga
    11461 tccggcgcag ttgttgttcg agatcgccgt gcggtcgccc cgcgcccggg tagttcacct
    11521 ggtgcgcgcg accatcgcgg ttccgttcgg tcccgactcg tcatcgccct cggttgcacg
    11581 gaccgggatg ctgcggcagg cggacgccgt agtaggcgtc agcgaatatg tagcccgcta
    11641 cgtacgcgag cacggaaaga tggacgcggt gcacgttccc atttcgctga tggatggtgg
    11701 cgagcccacg atgatgggga atccgggcaa ccggttcgtg acgctggtga atccctgcgc
    11761 ggttaagggc atttccatct tcacggctct ggcgaacgaa atgccggagg ttgcttttgc
    11821 ggcggtgccg acgtggggca caaacgcact cgattacgcg gaactgcggc ggcacgcgaa
    11881 cattaccatt attccgccgt cagacaatat cgacgaaatt ttgcggcaga cccgtgtgct
    11941 gcttgtgccc tcgctctggg ccgaggcgcg atcccgcatt gtcgtggagg ccatggcccg
    12001 cggtgtgccg gtcatcgcaa gcgatatcgg cggcatcccg gaggcgaaac ttggcgttga
    12061 gtacctgatt ccggtgaatc ccatttccac ttacgagcct gcggtggacg agcacatggt
    12121 tcccgtggcg aaggtgccgc cgcagaacat cgctccctgg cgggaggcgt tggagagact
    12181 gatacgggat ggggcgcatt atctcgatgt ctcactgcga tcgcgcgagg ccgcgctgag
    12241 ctatatcgca tctctgagcg tggagccttt cgaaagaata ttagaccgcg tgatcgcggc
    12301 gccgaagcgc ggtgcggcta cggccaagcc gccggcgcta tcgccggaga gacagcggct
    12361 tctggcgctg cggctgaggc agaagtccgc ggtgtccggc tggttcgcgg gtcccaccaa
    12421 agggaaaccg gttctcctct gtttccccca cgcgggagca ggcacggtgc attaccgcgg
    12481 ctggcaggaa gctctgccgg aagtcgcgct ctgcccgata cgtttgcccg gacgcgaaac
    12541 ccggatgcag gaaccgccaa ttgacgaaat gcgcgcgctg gtgaaggcac tggcgccgga
    12601 aatcgaacag cacgtgcagg cgacgttcgc gtttttcgga catagcatgg gcgccggaat
    12661 cgcgttcgag atggcgcgcg aattgcggcg gcgtggcgcg gcgatgccgg ttgctttgtt
    12721 cgtttcagcc gcgcgcgccc cgcagtggag gcttggctat cagccgccgc cggagccgga
    12781 agacgccgag cttgtggagc aactggcgcg gcttgggtcg atgtcgcccg aagtgctgcg
    12841 gctcgcctta cccgtgcttc gcgccgatac gcggctctac cggaactatg tgtaccagcc
    12901 ggaagatccg ctgccggttt ccctctgcgc atacggcggg gagcaggacg attcggttcc
    12961 ccggcaggac atcgaggcgt ggcgcgaaca agccggcggt acgttccgga tgaggatctt
    13021 tcccggcggt catttctacc tccaggagaa cccggccgcg ttcctgtccg cgttgcacgc
    13081 cgacctggat caggccggcg tcgtgggacc ccgctgatcc gtttcctgtt ccggaacggc
    13141 cggaggttcg gggagtttgc cggcgtagtt agccgccgcc caaaacaatg cagttgaagt
    13201 gctgagaact cccgcgatga cgccgatgcc gcgcaccggg taccgcaccg cggccacgct
    13261 tgccgcggtc atcgagatca tcatcgcgcc gttcaaaatc atctccatcg ccgagaacac
    13321 acgtccccgg aagcggtttg ggacgtgatg caacaacatg gtgcggttca gaatattgtt
    13381 cgctcccatt cctatccgcg atagagcgat aaacagaacg gcccaccaga cgtcagggga
    13441 gacggcgaag agaacgtatg cggcgccgtg gacaaacaac aaaatagcga tggcatgttt
    13501 gtagcgcacg aaatcaagat ggggtccaag ccaatgtgcg agaaaaccgc ctgcgaccag
    13561 gccgattccg gccgagcccc agatgaagcc aacgcctgat ggcccaagct tgaaaaccag
    13621 ttcaccgtac agcgtgaaca agatctgtgc cgcgcctcca ccgctcgccc atcccaccag
    13681 tacaagcgcg atgcccagca ccagcggggt cgaccggatg taggccatac cggagcggaa
    13741 gtcgtcccaa aaggcgcgaa cgcttcgcgc gggagcgcct gaatccgtgg cggcccggaa
    13801 acccgctccg gacggtctga gtctcgagat cgcacccgcg ctgaacagga acgagaaggc
    13861 gttcacgacg aacgcccact cgtaaccgaa tcgcatggtg ctggctccgc cgagaaaagc
    13921 gccgatggag agtgtcagcc atgcggttgt gagcgtgagc gcattggccg tgtgaagttc
    13981 ctcgggccgc gtgatgtccg gcagaatggc ggaacgccca ctggtgaaga acggcgacgc
    14041 gaacatcaaa ctggcactca ggaaatagag cagccattcc tgcttatgcg tgatcaccag
    14101 gacaaaagcc agcgccgcca cggcgcgaaa gagatcgctc acgatcatga cctgcttgcg
    14161 gtccatgcgg tccagcacaa ccccggcgat cggtcccgcc gcgatgccac cgagggtgcg
    14221 cgcgagcatt acacctccga ccgcagcgcc cgaaccagtg gtgtgtagtg cgaggctcaa
    14281 caccgcgatg gtgttgaaat gatcgccgac ttcactgacg atctgtccga gccacgtata
    14341 tcgataattt cggttaccca gcaggagagt ccaaaatgga gtgccgggtt gtcgggaagc
    14401 agtcagcaat tccccagtat agggagatgg tggaggcggc gggagtggta gtgtcagtat
    14461 tgccagttca ggtgggcttt agctatagtt tacttcgacg cctgccaatt ggagagaagc
    14521 tcaacgagat ccgcatgtcc attctcgcga gccagatcga tcggtatctt accgttatcg
    14581 gctgcggcgt tcgggtcggc tccgtgctcc agcaagatgc gcgcgaggtc cttgtgtcca
    14641 agggccgcgg gatgatgtaa cggtgtcaag gacccttcct gtttcacgtt cgggttcgcg
    14701 tgatgcgcca gcagcatgcg caccagccgc tctgccttgg ccaggtcgcg attcgctact
    14761 gcgctgtgca gggcggtcac cctcatctta ttctgcgctg gaatattagg atcggctccg
    14821 ctgcggagaa gcaagtccac cgcatccatg gcgccgaaga aacaggccaa cgtaagcgcc
    14881 ggaaagccat ctttggccgg cgcgttcagc agcgcggggg attgcgccag aaacgccgac
    14941 aacctgtctg tgagattcag ggcggctgcc tcgtggaatg caggctccgc tccgttgtcg
    15001 agaaacaatt gagtcacgtc aggccgcatc atgtagaccg agaacatgag cgcggagact
    15061 tcgttctcgt ccctggagtg aacgagtgac gaatcttcca cgagtgcttc tcaatcttaa
    15121 ttccgttcgt tcaggaacgt cagcacttta tgaatgccgg ccgaagaccg cacccgagcg
    15181 aagagattga acagagtcac cggagtggcc gtcggaatgt gccgcctgga gccacgcggc
    15241 gcgcgcttcc tcttctctgc caagcaattt cagcacgtgt cccaggttca ggagcgcctc
    15301 actgaattga gagttcagcg cgagggcctc cttgtaaagt ctgacagcat cttcgcgctg
    15361 tccggttttg tcgaggagca ggccggtgtt gaagtatagt tccggcgagg agtcgcccag
    15421 gtcgatgagc cttccgtgta agtcgagggc ttcgtcataa cgtcccaatt caatcgcgac
    15481 gccggccagc ccccgaaccg cttccaggga gccggggtgg agtttggcgg cttcgccgta
    15541 gtactggcgg gcatcttcca gatcgccggc gcgccaactg ctcagcgcca gattgagcag
    15601 ggcttccagc cactcggggc ggagccgcac cgcagactga aacgcctcga acgccgtccg
    15661 ggggtcctct tgcgcaaggc gcaggcagcc gagccggaac catgcttccg tgttttccgg
    15721 ctgaaccatc agcactttgg tatattgctt cgccgcggct tccgcatctc cggcctcttc
    15781 gaggacgccg gctaaaatca tggccagcgg catattttca ggctgttcgc tcaagccctg
    15841 ttcgcaacag gcgcgcgctt cttcgagagc accgcattga cgcaaggccg gtgcaatcac
    15901 agccgctgcc tgtgttttat cgcaccgcag ccgcatcgcc tcgagaaacg cctttgcggc
    15961 ttccagcggg tggtacgacc ggagaaggga aatacccaga ttaagccaga catttgcgtc
    16021 ttcaggaacc agttcaacca ggcttccaca atggcgcgag gcttcctcgt agtcctgccg
    16081 ccacaacgcg caagcagcca atcctctgat tgcaggggtg gattctggct gaagagaaat
    16141 caaccgttcg gaatactcgc gggtcaaggg ctcctgtgcc gtcgcaatcc cgatggccac
    16201 aaggttggta aggacctctg ccgaatccgg ctccgatgtg agaatttctt tgtactcctc
    16261 gatggcttcc tcgaatcggg agacgagttg aagcgccacc gctctcgcaa agcgcgctct
    16321 gccggaaacc ggtgcggccg cgtatagagg ctccagcgcc tcgagcgctt tcccgggctt
    16381 ctccaggtgc agatagcaaa tggccagtcc catgagggcg tccggagaag cgggatcaga
    16441 ttcgagcgtt cgctgaagca gctcgacagc ctctgtccag cggcctaacc tctccaggca
    16501 aaccgcggca ttcgtttgtg ccgtgagatc cacgggatcg agagaagcgg cagacagaaa
    16561 gctgctcagg gcatcctcgt aggatccttc ggcaaattgc acttcggcgc gcgcgaccgc
    16621 gtccgcttt
//
LOCUS       DASTMK010000235         8777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_523696, whole genome shotgun sequence.
ACCESSION   DASTMK010000235 DASTMK010000000
VERSION     DASTMK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523696"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /gene="epsC"
                     /locus_tag="VFQ79_10310"
     CDS             <1..455
                     /gene="epsC"
                     /locus_tag="VFQ79_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEU0140096.1"
                     /translation="IHPGARIGRRFFIDHGMGVVIGETTEIGDDVLLYQGVTLGGTGK
                     EKGKRHPTLGNRVVVGTGAKVLGNIRIGDGVKVGAGSVVIHPVPDHSTVVGIPGRVVR
                     SRGSEETLEHGNLPDPEGQALDELARRVSDLEAQLKLLADERMSERRG"
     gene            complement(562..3369)
                     /locus_tag="VFQ79_10315"
     CDS             complement(562..3369)
                     /locus_tag="VFQ79_10315"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU0140097.1"
                     /translation="MSDGDRITQQEKTAPYRQDVHAVIAALGSDGHRGLTEQESGKRL
                     EQYGRNELTAEKKIPGWRRFLAQFQDALVILLLVATAISAGLWLYERESALPYEAIAI
                     FSVVLLNAAMGYVQEARAESAVAALKQIAAAHANVIRNGERRSIPAAEVVPGDIILIE
                     EGDTIPADARVIQSSSLKCAEAALTGESLPVVKDPALITEEAGIGDRDNMIFSGTSAT
                     YGRGRAVVVTTGMDTEMGRIAGMLKEAPDEATPMQKELDRVGKLLGAGVVVIALVMIV
                     TIILFEQVRGWSAIFDVLILGIALAVAAVPEGLPAVVTAVLALGVQRMAKRKAIMRHL
                     SAVETLGSATVIASDKTGTLTKNEMTVQAVVTASGRVRFDGTGYAPEGPVHREDGGAV
                     DGPLRIELVRALAIADRANNAALRHNEGRWTVQGDPTEAALVVAARKAGLEEEALSGR
                     FERVGEVPFSSERKLMSTLHTDTKRQERLLVFSKGAPDVLLSRCSEELVGEGTRPLTD
                     HRRAEIQASNEELAAQALRTLAMAYRSVPAESVDIEDIDERVERNLIFAGLIGMIDPP
                     RSEAKDAVAEAKSAGIRPIMITGDHPVTAQVIARELGIADGGRAVTGAELQETSGEAL
                     VDLAKRVSVYARVNPEHKLRIVRALQSNGAVVAMTGDGVNDAPALKTADIGVAMGITG
                     TDVSKQAADMVLADDNFATIVAAVEEGRSIFSNIRKFLRFLLSSNMGEVMTMFFGVLL
                     ANVIGLQAEESAVVLPLLATQLLWINMVTDGAPALALGVDPPDHAVMGKPPRPAGERV
                     ITGRMWTGILFVAVIMAAGTLIVLDASLPGGFIPGSGSMRYAQTMAFTTLMMYQLFNV
                     LNARSDERSAFAHLFSNAWLWGAIGLAILLQIAVVYIPVLQQAFSTVSLKATDWLRCA
                     AVASSVLWLREISKFLNRRSS"
     gene            complement(3524..4846)
                     /locus_tag="VFQ79_10320"
     CDS             complement(3524..4846)
                     /locus_tag="VFQ79_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140098.1"
                     /translation="MRVSRLVSSIFGLALLTGFPVSLHSQNPPAGGGQGAPPPQQSAP
                     PQGGSVPGGSIPGREPTPGRQPGRDPFPRQDQMDPNSRMPMPEMQRPIFLSGKVVMDD
                     GTPPPDSVTIERVCNGIARPEGYTDSKGRFSFQLGQNAGVFQDASVSSQADPGFGGRG
                     MDSRNRQGGMGFPGNTRGISERDLMGCELRASLPGFRSEIVNLSGRRVMDNPDVGTII
                     MRRLGNVEGLTISATSALAPKDARKAFDKGRKAIEKKKWEEARKELNKAVEVYPKYAA
                     AWFNLGLVHEQNKNMDDARKAYAQALEADAKYVNPYLQLADIAAREQNWQEAADTTDR
                     LLKLNPFDFPRAYLINAVANLNLQKIDAAEASAREAVKLDTGHQIPQVSHVLGVILAQ
                     KRDYPGALAQMRAYLEHAPKAGNAQQVREQITQIENLMGKTTTAQEQK"
     gene            4941..5570
                     /locus_tag="VFQ79_10325"
     CDS             4941..5570
                     /locus_tag="VFQ79_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140099.1"
                     /translation="MKKSLVLIASPLLLLLAGCSESPKPAAEQKKKEPEKAPEPVTGR
                     SAFQQMFITARNWSPDAVGLRVQSIRLDDVKDEPGKSGAWQAVFVSPSRGRARSYTYS
                     VIEGPGNLHKGVFAGPEEGYREGGQAQPFPVAALRVDSNEAYETALNKGKGNEYSKKN
                     PDMPITYLLEKTPRFPNPAWRVIWGESVGTSNFSVYVDASTGNYLQTMR"
     gene            complement(5575..6165)
                     /locus_tag="VFQ79_10330"
     CDS             complement(5575..6165)
                     /locus_tag="VFQ79_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0140100.1"
                     /translation="MRNLFAVLLAGGLMYAQAPGQGERGRAMSDLHATRRLFLDAVGP
                     LTPAQWKFKPAPDRWSIAEIAEHIALSEDMIFQVVTEKILKSPADASKKSEVKDEEVL
                     RIVADRSTKATAPERLQPTGRWPTKQAVMDHFKASRDRTIEYIQTTQDPLRRHFMQNR
                     TAGMLDAYQWILVISAHSQRHIDQIKEVMAQPGFPK"
     gene            complement(6210..7511)
                     /locus_tag="VFQ79_10335"
     CDS             complement(6210..7511)
                     /locus_tag="VFQ79_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0140101.1"
                     /translation="MMRSLFAKILLWFVATTLISALAVVLTTMVGLSAPRARHQPFAM
                     LLTFQLSQARQAYEQGGAPALKETLERLRAGGLSRGMFLDSSGRDLMTGQDRSDLLRE
                     SEDAIGLPVRRGDQVLIGRPDDDGKYWFFIQIPRRRWFLWFLRPQYLWILGVAVFFCY
                     LLARHLTTPVRQLQTAVRAFGHGDLRARAASGRKDELGQLAQTFDQMADRIETLLTAE
                     RRLLLDLSHELRSPLARLNVAVELARSGEDRERALDRIQKESDRLEALVSELLQVTRA
                     EGDPETRRKEILPLDELVEDVVKDAVIEAESRGCGLRFTNGQPVSVCGDPELLRRAVE
                     NVVRNAIRYSPRETTVEVSLGRTPKSALVRVRDYGPGVPEDALPRIFDPFYRVAADRG
                     RDSGGVGLGLSIARRAVELHKGRLHAENKKPGLMVEIEIPV"
     gene            complement(7512..8204)
                     /locus_tag="VFQ79_10340"
     CDS             complement(7512..8204)
                     /locus_tag="VFQ79_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0140102.1"
                     /translation="MHILVIDDDIELCSLLREFLQREGFKVECEHDGARGLERALTCG
                     CNLVVLDVMLPGLDGFEILRRLRKQSRVPVLMLTARAEDVDRIVGLELGADDYLPKPF
                     NPRELAARIRAILRRIDSQPSGRGRIEVNGVILDPESRQVTYRGTEIELTTFEFDILE
                     MLIRSAGRVLSRDALMESLYSRKSSPFDRSIDMHVSHLRKKLNGEQNLIKTIRGVGYQ
                     FVKSAAGDGAEE"
     gene            8305..>8777
                     /locus_tag="VFQ79_10345"
     CDS             8305..>8777
                     /locus_tag="VFQ79_10345"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU0140103.1"
                     /translation="MAFVPAEARLKDPYEFDAADVSAPPTGLLQSLKRIGPGMILAAS
                     IVGSGELIATTTLGAEVGYVALWVILISCFIKPAVQAELGRYTIATGKTGLGGFNEVP
                     GPKWRVSWVVWLWLCMIAMTRFQIGAMFGGVAQVMKLIFPAVPINAWVVVLLAITL"
BASE COUNT         1706 a         2764 c         2525 g         1782 t
ORIGIN      
        1 aaatccaccc cggagcgcgg atcgggcggc gattcttcat cgatcacggc atgggagtgg
       61 tgatcggcga aaccaccgag atcggcgatg acgtcctgct ttaccagggt gtaacgctag
      121 ggggtaccgg taaggaaaaa gggaagcggc atcccacgct gggtaatcgc gtggtagtgg
      181 gcacaggcgc caaggtgctg gggaatatcc gcatcggaga cggcgtaaaa gtcggtgcag
      241 gctccgtcgt cattcatccc gtaccggatc attccacagt cgtcggcatt cccggacgcg
      301 tggtccgctc caggggcagc gaggaaactc tggagcatgg caatctgccg gatccggaag
      361 ggcaggctct cgacgaactc gcaaggcgcg tgagcgactt ggaagcacaa ctgaaactgc
      421 tggccgacga aagaatgagc gagcggcgcg gctgaggcag ctaaaagccg cttgaggtgc
      481 cttcggcagc agcgcgaaca tctcgagttc gcaatgcgta ccttcggtgt gccacaaggg
      541 cgcgagttct ggccgcgcac ttcaggagct tctgcggttc agaaacttgc tgatttcgcg
      601 cagccacagt acggaactgg ccacagcggc gcatcgcagc caatcggtgg ccttcaagga
      661 cacggtagag aaggcctgtt gaagaacagg gatgtagacc actgcgatct gcagcaggat
      721 tgcaagtccg atggcgcccc agagccatgc attgctgaac aggtgcgcga atgcgctgcg
      781 ttcatccgat cgcgcattca gcacgttgaa caattgatac atcattagcg tcgtaaaagc
      841 catcgtctgc gcgtaccgca tgctcccgga acctggaatg aatccgcccg gcagcgaggc
      901 gtcgagcaca atcagcgtgc ccgcggccat gatcacggct acaaagagaa tccccgtcca
      961 catacgcccg gtaatcactc gctctcccgc gggacgcggc ggtttgccca taacggcatg
     1021 gtcgggggga tcgacgccca atgcaagagc cggcgctcca tcggtgacca tgttgatcca
     1081 caacaactgc gtcgccagta gcggcagcac aacggcgctt tcctcggcct gcaaaccgat
     1141 cacgttggcc agcagcactc cgaagaacat ggtcatcacc tcacccatat tggacgaaag
     1201 caaaaagcgc agaaacttcc ggatattgga gaagatcgaa cggccttcct caacagcagc
     1261 cacgatcgtc gcgaagttgt catccgcgag aaccatgtcg gccgcctgtt tcgaaacatc
     1321 ggttccggtg atccccatcg ctactccgat gtcggcggtc ttcagagccg gagcatcgtt
     1381 gactccgtcg cctgtcatag caacgaccgc gccattgctc tggagcgcac ggacgattcg
     1441 cagtttgtgc tcgggattga cacgggcgta caccgaaacc cgctttgcca gatccacaag
     1501 cgcttcgccg gacgtttcct gcaactcggc gcctgtgaca gcgcgaccgc cgtctgcaat
     1561 cccaagttcg cgcgcaatca cctgggccgt taccggatgg tctcccgtga tcatgatggg
     1621 gcgtatgccg gcgctcttgg cctccgcaac cgcgtccttc gcttcagagc gggggggatc
     1681 gatcattccg atcaggccgg cgaaaataag attccgctcc acacgttcgt cgatgtcttc
     1741 gatgtccact gactcggccg gaacagatcg atatgccatg gcgagtgttc tcaaggcctg
     1801 agcagcgagt tcttcattgc tggcttggat ttccgcgcgc cggtggtccg ttagaggtcg
     1861 cgtgccctcc cccacgagct cttctgagca gcgggacaga agcacgtccg gtgcgccttt
     1921 cgaaaacacc aggagacgct cctgcctctt ggtatccgta tgcaaggtgc tcatgagctt
     1981 ccgctcggaa gagaaaggga cttctccgac ccgctcgaag cgcccgctca gcgcctcctc
     2041 ttcgagaccc gctttccgcg cggcgacgac aagcgcagct tccgtcggat cgccctgaac
     2101 cgtccagcgg ccttcattgt gccgcagggc ggcattattg gcacgatcgg cgatggctaa
     2161 tgcgcgcacc aactcgatcc gcagcgggcc gtcaacagcg cctccatctt cgcgatggac
     2221 cggcccttcg ggagcgtagc cggtcccgtc gaatcgcacg cgcccgctgg ctgtcaccac
     2281 ggcttgtacc gtcatttcgt tctttgtcag cgtgccggtt ttgtccgatg cgataacggt
     2341 tgccgatcca agggtttcga ccgccgagag gtggcgcatg atcgctttcc gtttggccat
     2401 gcgttgcaca ccgagagcca gtactgctgt caccacggcc ggcaggccct cggggaccgc
     2461 tgctactgcg agggcgatgc caaggatcag aacatcaaaa atcgctgacc accctcgcac
     2521 ctgctcaaag aggatgatgg tcacaatcat caccagggca atcaccacaa caccggcgcc
     2581 caacaacttg ccgacgcgat ccagttcttt ctgcatcggt gtggcctcgt cgggcgcctc
     2641 tttcagcatg ccggcaatac gacccatttc cgtgtccatc cccgtggtca cgacaaccgc
     2701 ccggccgcgg ccgtacgtcg ctgacgtacc gctgaagatc atgttgtccc gatctccgat
     2761 acctgcctct tcggtgataa gggccggatc tttcacgacg ggtaagctct cgcccgtcag
     2821 cgccgcctcg gcgcacttga gcgacgatga ctggatcaca cgagcgtccg ccggtatggt
     2881 gtctccttct tcgatcagga tgatgtcgcc gggtaccacc tcggcggcag ggatgcttcg
     2941 cctttcgccg ttgcgaatga cgtttgcatg ggccgcagct atttgcttca acgcggcaac
     3001 cgccgattcg gctcgagcct cctgaacgta acccatcgca gcgttaagca gcacgaccga
     3061 gaagatcgcg atggcctcgt aaggcagtgc cgattccctc tcgtaaagcc agaggcctgc
     3121 ggagatcgcc gtcgcaacca gcagcaggat gacaagcgca tcctgaaact gtgccaggaa
     3181 cctgcgccag cccgggatct tcttctccgc ggttagttcg ttcctgccat attgctcgag
     3241 ccgctttccg gattcctgct cggtgagtcc gcgatgtcca tcgctgccga gggccgctat
     3301 aacggcatgc acatcttgcc ggtaaggagc cgttttttcc tgctgcgtga tccgatcgcc
     3361 gtctgacatt atgtcaagta tgccctgagc gctggcgccg cgcttggatc ataacagggg
     3421 ggtagggaat ttcaccgcaa cttcgacagg cccagctacg cccgagccag cccgcgcggt
     3481 tccgtttctg agccgcgacg gccacggagc ggtggctgca acgttatttc tgttcctgcg
     3541 cggtggtcgt ttttcccatc aggttttcga tctgcgtgat ctgctctcga acctgttgag
     3601 cgttgccggc tttcggagca tgctccagat aggcacgcat ttgggccagc gcgccgggat
     3661 aatcgcgctt ctgcgcaaga attacgccca gaacgtgaga cacctgcggg atctgatgcc
     3721 cggtgtccag cttcaccgcc tcacgagcac ttgcctccgc cgcatcgatc ttttgcagat
     3781 tgaggttggc cactgcattg atcaggtacg cccgtggaaa atcgaacggg ttcaacttca
     3841 gtaaacgatc cgtggtgtcg gcggcttcct gccaattctg ctcccgcgcg gcgatatccg
     3901 ccagttgaag ataagggttg acgtatttag catccgcttc cagcgcctgt gcgtatgcct
     3961 ttcgcgcatc gtccatgttt ttgttctgct catgaacgag ccccaggttg aaccaggcgg
     4021 cggcgtactt cggataaacc tcaaccgctt tgttcaactc tttccgcgcc tcttcccatt
     4081 tctttttctc gatcgctttg cggcctttat caaaagcctt ccgggcatct ttcggagcca
     4141 gcgcgctggt ggcgctgatc gttaacccct ccacattgcc caggcgcctc atgataatgg
     4201 tgccgacgtc agggttatcc atgacccggc gtccgctgag gttcacgatt tccgagcgga
     4261 atccaggcaa agatgcacgt aactcacaac ccatcaggtc gcgttcgctg ataccgcggg
     4321 tgttgccggg aaaccccata ccgccctgac ggttcctgga gtccatcccc cggccgccaa
     4381 accccgggtc ggcctgcgag ctcacgctcg cgtcctggaa tacaccggcg ttttgtccaa
     4441 gttggaaact gaaccggccc ttggagtcgg tgtaaccttc gggtcgcgct atgccgttgc
     4501 agacgcgctc gatcgttacc gaatcgggcg ggggcgttcc gtcgtccatt acaaccttcc
     4561 cggaaagaaa gatcggccgc tgcatttccg gcataggcat acggctgttg ggatccatct
     4621 ggtcctgccg tggaaatggg tcgcgtccgg gttggcggcc aggcgtcggc tcccggccgg
     4681 ggatcgaacc tcccggaacg cttccacctt gtggcggagc gctctgttgc ggcggagggg
     4741 caccctgccc accaccagcc ggaggattct gtgagtgaag agagacgggg aaacccgtca
     4801 gaagcgccag accgaagata ctgctaacaa gtctgcttac gcgcattggg atcacctccc
     4861 gagtcgactt tccttattat gccataggcc cacgagcaga accgggacgc ttctgtaaaa
     4921 tggtttgagt gacggcaacc atgaaaaagt ccttagttct cattgcaagt cctcttctcc
     4981 tgctcctcgc cggctgttcg gaatccccca aacccgcggc cgaacagaaa aagaaagaac
     5041 ccgaaaaggc acccgagccc gtaacgggca ggtccgcttt ccagcagatg ttcattaccg
     5101 cgcgaaactg gtcgccggat gccgtgggct tgcgcgtcca gagcatacgt ctggacgacg
     5161 tcaaagacga gcctggcaaa tcgggagcct ggcaggccgt cttcgtctct cccagccgcg
     5221 gacgggcccg cagttatacc tactccgtaa ttgaaggtcc gggcaacctg cataagggcg
     5281 tatttgccgg accggaagag ggctatcggg agggtggaca ggcgcagccg ttccctgtcg
     5341 cggcattgcg ggtagattcg aatgaagctt acgaaacagc ccttaacaaa ggaaaaggga
     5401 atgagtattc gaaaaagaat cccgacatgc ccatcaccta ccttctcgaa aagacccccc
     5461 gctttccgaa tccggcctgg cgagtgattt ggggtgaatc cgtcgggacc agtaattttt
     5521 cggtgtacgt agacgccagc accggaaatt acctgcaaac catgcgctga tgcgctactt
     5581 cggaaagccg ggttgcgcca tgacctcttt gatctgatcg atatgtctct gcgagtgcgc
     5641 cgagatcacc agaatccatt ggtaggcatc cagcatcccg gcggtgcggt tttgcatgaa
     5701 gtgccggcgt agagggtcct gcgtggtctg aatgtattcg atggtgcggt cccggctggc
     5761 tttgaagtga tccattaccg cctgtttggt gggccagcgg ccagtgggtt gcaatctttc
     5821 cggggcggtt gctttcgtgc tccggtccgc cacgattcgc agcacttctt catccttcac
     5881 ttcggacttt ttggaggcgt cagccggcga tttcagaatc ttctccgtga caacctgaaa
     5941 gatcatgtct tcgctgaggg cgatgtgctc ggcgatttcc gcgatcgacc atcgatccgg
     6001 cgccggtttg aacttccatt gcgccggtgt gagcggcccc accgcgtcga gaaacagcct
     6061 ccgcgtggcg tgcagatcgc tcatggcgcg gccgcgctcg ccctggccgg gcgcctgtgc
     6121 gtacatcaac cccccggcaa gtagaacagc gaataagttc ctcatatcac ctcgaaggag
     6181 ccattttcgc acgcgggcac caccaagact cataccggga tttcgatctc gaccatcaga
     6241 cccggctttt tattttcagc gtggagtctc cctttgtgaa gttcgacagc ccgccgcgca
     6301 atcgacagcc ccaggcccac accgccgctg tcgcgcccgc ggtccgccgc cacgcggtag
     6361 aacggatcga agattcgcgg cagggcatcc tcgggaactc ctggtccgta atcccgcaca
     6421 cggaccaggg cggatttagg ggtcctgccc aggctcactt ccactgtggt ttctcgtggc
     6481 gaataacgaa tcgcattccg cacaacgttt tctacggccc gtcgcagcag ttccggatct
     6541 ccgcatacag acaccggttg tccgttggtg aaacgcagcc cgcagccccg ggactccgcc
     6601 tcgatcacgg catctttcac gacatcctcc actaattcgt ccagtggaag gatctccttt
     6661 ctcctggttt caggatcgcc ctccgcacgc gtgacttgca gcagttccga gaccagcgct
     6721 tcaagccggt ccgattcctt ttggatacgg tcgagcgccc gctccctgtc ttcgcccgag
     6781 cgcgccagtt caaccgccac gttcagcctg gcaagcggcg atcgcaactc gtgagacaga
     6841 tcgagcagca ggcgccgctc cgccgtcagc agcgtctcga tccggtcggc catctggtca
     6901 aacgtctgcg ccagttgacc cagttcatcc ttgcgccccg acgccgcgcg cgccctcagg
     6961 tcgccgtgcc cgaatgcgcg gaccgctgtt tgcagttgcc gcaccggggt ggtcaggtgc
     7021 cgtgccagca gatagcagaa gaacaccgca acgccgagga tccacaggta ttgcggccgc
     7081 aggaaccaga gaaaccagcg ccggcgcgga atttggataa agaaccagta cttcccgtcg
     7141 tcatcgggcc ggccgatcag cacctggtct ccacgccgca ccggcaaccc gatggcatct
     7201 tccgattcac ggagcagatc ggagcgatcc tgcccggtca tcaggtcgcg accggaagaa
     7261 tcgaggaaca tgccccgcga aaggccgccc gcccgcaagc gttcgagcgt ttctttcagt
     7321 gcgggcgcgc ctccctgttc ataagcctgc cgcgcctgcg acaactggaa ggtcaggagc
     7381 atggcgaaag gctgatgccg cgcgcgcggg gcggacagcc ccaccatggt ggtcagaacg
     7441 acggccagcg cggaaatcag tgtagtcgcc acgaaccaca acagaatttt cgcaaatagg
     7501 cttcgcatca tttactcctc ggcgccgtct ccggccgccg atttcacaaa ctgatatccg
     7561 accccccgaa tggttttaat cagattctgc tccccattga gcttctttcg cagatggctg
     7621 acgtgcatgt cgatggaacg gtcgaaagga ctcgatttcc tgctatacag gctctccatc
     7681 agcgcgtccc gggatagcac gcgcccagcc gatcgtatca gcatctccag gatgtcgaac
     7741 tcgaaggtcg tcagctcgat ttcggtgccg cggtaggtca cctgtctcga ctccgggtcc
     7801 aggatgacgc cgttgacctc gatacggccg cggccggagg gctgcgaatc gatacggcgc
     7861 aagatcgcgc ggatccgcgc cgccagctcc cgcggattga acggcttcgg cagatagtcg
     7921 tcggcaccca gctcgagacc tacgatgcgg tctacatcct ccgcccgcgc ggtcagcatc
     7981 aacacgggga cgcggctttg tttcctcaaa cggcgaagaa tctcaaaccc gtccaggccg
     8041 ggaagcatca catccagaac caccaggttg cacccgcagg taagcgcgcg ctccaggccg
     8101 cgcgccccgt cgtgctcgca ctcgaccttg aaaccctccc gctgcagaaa ttccctcagg
     8161 agcgaacaga gttcaatatc gtcatcgatt acgagaatat gcaccgcttt gaatttatca
     8221 aaagcaccgg cccgagtcgc acaggccggt gctctgcgcg atcttcattt gaattcccgc
     8281 cggcaaacgg gatagagtgg ccgcatggcg tttgtgccgg ctgaagcgcg gttgaaagat
     8341 ccttacgagt tcgatgccgc cgacgtgtcg gcgcccccta caggcttact ccagtctctg
     8401 aagcgcattg gccccggcat gatcctggcg gcctcgattg tcgggtcggg agagctgatt
     8461 gccactacga cgctcggcgc cgaggtcgga tatgtggccc tgtgggtcat cctcataagc
     8521 tgtttcatta agccggcggt gcaagcggaa ctcggccggt acacgatcgc caccggaaag
     8581 acaggtctcg gcggtttcaa tgaagtcccc gggccgaaat ggcgtgtgag ctgggtcgta
     8641 tggctgtggc tctgcatgat tgccatgacc cgcttccaga tcggcgccat gttcggaggc
     8701 gtcgcccagg tgatgaaact tatttttccc gctgtcccca tcaatgcctg ggttgtcgtg
     8761 ttgctggcga ttacgct
//
LOCUS       DASTMK010000236        18244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_202976, whole genome shotgun sequence.
ACCESSION   DASTMK010000236 DASTMK010000000
VERSION     DASTMK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18244
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202976"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFQ79_10350"
     CDS             <1..195
                     /locus_tag="VFQ79_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140104.1"
                     /translation="CQNLEAGDLLVIPVSAKSNRAVGRSSPARKVSATPKKKVARTTR
                     PTSGLKKISALQKSSTRVSR"
     gene            331..1074
                     /locus_tag="VFQ79_10355"
     CDS             331..1074
                     /locus_tag="VFQ79_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140105.1"
                     /translation="MFDRLLFSPDDDKADDYEDDEQIGLDSELEEYGPDEDEDEDEEE
                     EDDVVVLVGRDEEDEDEDEEEEENVARADSESDEGEKVAVEVPIPVVEPLTPPVAVKQ
                     GGGERRRSVKKAAVKKAAGKAATTKKSAAKAGAKAVAKKSSKATSTAKKKAAGKASTR
                     KAPAKKAPVKKTAAKKAAGKKAPARKAPVKKGAAKKAPVKKGAAKKAPGKKTAAKKKT
                     VKKAPARKSAAKAGAARKKGGAKKAGRRR"
     gene            complement(1132..1380)
                     /locus_tag="VFQ79_10360"
     CDS             complement(1132..1380)
                     /locus_tag="VFQ79_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140106.1"
                     /translation="MLNYGFHYKQEEPESMAGDKSESPTRSKEEIALELMKFISVTTG
                     YGKGTAGAGFANKPAGNAEEYAESLLQLYDRCLSAVSK"
     gene            1415..2245
                     /locus_tag="VFQ79_10365"
     CDS             1415..2245
                     /locus_tag="VFQ79_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEU0140107.1"
                     /translation="MPVNAVRFVRKMRGGAQAHLLEADDGGFYVVKFRNNPQHRRILV
                     NEWIASVFLRYLGILTPETQIVSLSSEFLRDNPEVGLQFGTKTVPIPPGWHFGSRYPG
                     HPDRTAVYDFLPDALLQKVENLDHFAGTLVLDKWMGNADARQSIFLRVQLKEWNLNKT
                     HPARKGFVAIMIDHGYVFDGPQWTFSDSPLQGLYFRPLVYRGVRSEESFEPWLSRVST
                     FPEEVMDEAWRQIPKEWLEGDEDELEQLLTKLLRRRKNLPAYLRDSAKARTHPFPDWR
                     "
     gene            complement(2481..3311)
                     /locus_tag="VFQ79_10370"
     CDS             complement(2481..3311)
                     /locus_tag="VFQ79_10370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0140108.1"
                     /translation="MPDVIVVGAGVIGLSIAWRLAQEKITVAVLDSGAAGGQASTAAA
                     GMLAPGGEIKRRTGWSQFALDSFRLYTSFVNELQVESDTSIEFRRCGGAELAYSESEW
                     ARLVERAATQRLLGVPTDPVTGPPLSSLVPGLELNSVIGAMFYPEDALVDPHHLVRAL
                     HAACISREVQVRQQLPVLGLRFDSGVVEVRTDEGVLRAPAAVLAAGAWSSSIPIEGAE
                     VPRCGPAKATCWLITSIPARSIPFSGTVRRTSCSVPEASPSPEAPWKMWASTQRWTRL
                     "
     gene            3377..4249
                     /locus_tag="VFQ79_10375"
     CDS             3377..4249
                     /locus_tag="VFQ79_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU0140109.1"
                     /translation="MHKFILHNGQIHEASSKLLSPGQVGALSGWGVFSTIRIFDGVLF
                     AVERHWARMKKDAEIMRVPFPADPRQVEDSLLELVEANHASNAALRIAVVRNRGGIWE
                     GPGIKRDFDVVALTTNVRNWGQGVKLGIAAQARHAQSRFAGVKILSWAENLVLYEDAH
                     SQGYDEVVLLNERGEVSECTSANIFCSNGNRVLTPPLVSGCLPGVTREVLLTEVRVAG
                     ISIEERVLTPTDLEAADEVFITSTTRQLLPVLSIEGLAIRTKGHAREKLQAAFSEYVD
                     RYVAAHRGAGTKTR"
     gene            complement(5047..6153)
                     /locus_tag="VFQ79_10380"
     CDS             complement(5047..6153)
                     /locus_tag="VFQ79_10380"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEU0140110.1"
                     /translation="MRSILLTLLLASLGQGAGKWELQYFYDKLDSQLVFQDLVFPSEQ
                     RGIAIGTLTEKGRERPVVVLTGDGGRTWEMQRLRETPISLFFLNDSLGWMVTEKGIWQ
                     TQEAGRDWKKLKGIRDIAEVFFTTPEHGWAIGGKKQVLETHDGGRRWTGVAAAEIPKG
                     NPKDSAYRCIDFATPQVGIIVGSSRPQRRDEPQLPEWIDPERGKRPEWPTLTLLLQTA
                     DAGKSWQASNSSLFGTVTSLDLRRDGFGLALFQFQGSFQYPAEVYRLDLHTGKSDRVF
                     RQKDRAVTDLVWIPGGSAYLGAIEPPGTLMHLPVPGRVRILSSDDLSKWSEMEVDYRA
                     TATGVILAAAGPDHVWAATDTGMILKLNRKPAPQ"
     gene            complement(6167..6769)
                     /locus_tag="VFQ79_10385"
     CDS             complement(6167..6769)
                     /locus_tag="VFQ79_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0140111.1"
                     /translation="MPKSKDLEFRLERTEQQLRLFQKISRFMVRESSLQEVLQGIVSL
                     VVEFTQCDSCLVYLIDNDELVLCASNTAHSAAVGNVRLKMSEGLTGWVARERRLLAIS
                     REVYKDPRFKYFRDLPEDTYEAFLSAPVIARNRVVGVINVQHKLPHAHSGSEMELLTT
                     VGEQVGCLLVLARMEPTAVEAANHVELVLSSGPAAPERQA"
     gene            6879..8471
                     /locus_tag="VFQ79_10390"
     CDS             6879..8471
                     /locus_tag="VFQ79_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU0140112.1"
                     /translation="MAHPRRSLFYLPLTLILFSLLGGLFGPGLAPTAAATSDDDIRQS
                     VETFTKVLSVLEENTAEPIDTDKAIYNGAISSMLRTLDPHSSFFDPKLFQVLQEEQRG
                     QYFGVGMLVGARDGGVVVVYPFSNSPAFQGGIHPGDIIIAVDGKPTVKASTSEVADLL
                     KGPRGTTVQVTVRRRGATGPLRFTLVRNSIPRATVQNAFRIRPKIGYVRIESFNENTS
                     REFEESLRELDESTLEGMVLDMRSNPGGLLREGVAIADRFLRKGQLIVSHRGRASAER
                     PYVAKRGNDGREYPLVVLVDRTSASAAEIVAGALQDHDRAWIVGEPTFGKGLVQSQYP
                     LSANTALLLTTAKYYTPSGRLIQRDYSNRSFFEYYAHKDLTERDPKDMKATDSGRPVY
                     GGGGITPDEEYTREKLNAFQTALLRRDAFANYTAQYFSSRKTELPADWTPGREIEDDF
                     HQFLLKEGVSFTEADFAANHEWIRQRLHVDMYLTAKGKDSADRLSIEIDQQVAQAIDS
                     MPKAKALIDAAKGILAKRAELQ"
     gene            complement(8505..9731)
                     /locus_tag="VFQ79_10395"
     CDS             complement(8505..9731)
                     /locus_tag="VFQ79_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140113.1"
                     /translation="MIRRREFLAVPGLAPLAAFQTAPKTVAAIITEYRHNSHADVIAG
                     RLLEGYDFDGRRRQPRVKMVSMYTDQVPANDMSRAMAAKHAVAIKPTIAEALTLGTGK
                     LAVDGVVLIGEHGNYPTNEKGQKLYPRYELFSKIVEVFRSSGRSVPVFTDKHLSVEWK
                     KAKWMYDQSRELGFPMMAGSSLPVTWRHPPVELKKGTPVRHAVAAFYGGKEAYGFHAL
                     EALQCMVERRQGGETGISQVHAIEGPDVWKWTSENAWAGPLLESAVARSETRKAGPMR
                     EVTKNPAVFVLRYRDGLEAAVYMLNGYLDQCVFATNLPERKEPLATEIWLQPGRPFSH
                     FSALTYYIEELVLTGKPSYPVERTLLTTGALATLMDSSYEHRKMETPHLDVKYQAPEK
                     SLFSRGPVPPPEKAKA"
     gene            complement(9833..10324)
                     /locus_tag="VFQ79_10400"
     CDS             complement(9833..10324)
                     /locus_tag="VFQ79_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140114.1"
                     /translation="MNWKWMPAAVLCLTGMAVPAAAQRSHGYWFVAPGGGTSGGRTQF
                     SLHMGGGGEIALKRGVALGLEAGAWGLPRDYVDSVIGIASFNGTYHFLPSRDDKWDPF
                     ATAGYSLAFRSGTRNMVNYGGGFNYWFQPSVAVRVEFRDHYSDRLHLWGFRFGLSFRD
                     LMP"
     gene            complement(10416..11594)
                     /gene="dgoD"
                     /locus_tag="VFQ79_10405"
     CDS             complement(10416..11594)
                     /gene="dgoD"
                     /locus_tag="VFQ79_10405"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEU0140115.1"
                     /translation="MKITQVKTVVVNARMRNWVFVKVETDQDGLWGWGEASLEWKTRS
                     VAGAVEDLAPMILGEDPTRIEHLYQKMYRQSFFRPGVIGMSAISGIEQALWDILGKIT
                     GMPVYRLLGGAVRDRVRMYTHLGGGEMKAVYESQHSGDASAMVDLALEVAARGYTAVK
                     VLLTPPTESLNGAALYRHAESRMEALRRALGDDIDIMVDCHGRHFPANALEFCRVLAP
                     YRPFFVEEPVPPENVDAMAEVKRSSPVPIATGERLVTRFEFRTLFEKQACHIIQPDLC
                     HCGGLWEAKKIAAAAETYYVGVAPHNPLGPVANAAALHFALSTPNFLIQEDMLSDVPW
                     RWDVVRHSLTSKDGYWLPSDVPGLGVEVDENAARLHPFEQEVIQSLAARAPDGAVLDW
                     "
     gene            11606..13210
                     /locus_tag="VFQ79_10410"
     CDS             11606..13210
                     /locus_tag="VFQ79_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0140116.1"
                     /translation="MAIGRGCYHDALNGNSVDRLSETTYDEVAYPTFPRFQTHPAHLQ
                     TMAFLGGLKSAPPGRCRVLELGCGDGGNLLSMAVALPESRFVGVDLAGTAIERGNSTV
                     TALGLANMRLMQADLLNIGASIGEFDYIIAHGLYAWVPDPVREKLLAICASQLAPDGI
                     AFISFNSFPGCHLRLALREMLLFHIQHIRNRQQRLAEARSLLKALSSALVADPRGEAI
                     RKEVERLIDLEDGYLFHDDLAEVYRPVYFHEFAAHAQRHGLRYLCDARYWHMEESPLT
                     DVAGALSETAGEDRDLRAQYVDFFKVRPFREALLCKGDRTPDFRALSGRFQNLFVASS
                     SAPVGEPDAEGGLRFQIAEGRSLSTNHPIAKAALAYLGRVWPRWARFPDLLDEILAPD
                     VDEDARRSSGEALSEMLLRLSAVRFVQALTTPPRLASQLSERPVASPLVRYQLSTGSA
                     KVSSLRHTTVELSDPLSRHTVRLLDGTHNRAELLEELVALVKLGVAKLEINKEQITDP
                     ARVPDLLARSLEGNLEKLMRLGLLSA"
     gene            complement(13238..14872)
                     /locus_tag="VFQ79_10415"
     CDS             complement(13238..14872)
                     /locus_tag="VFQ79_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0140117.1"
                     /translation="MKQKLPTRRMREHPDLEQLKRQAKQLLRAFKAGDPAAAGEVNAH
                     YDGAQAAVFALHDAQLVLARSYGFDSWPKLKAYVDGVTIRRFVEAVRAGHAGRVRTML
                     KTRPELANMTVSYGDEHRAIHYAVMHRHPEITRLLMQKGANARAGIHPHRDATSAWIL
                     ARERDYDEIVAIIEEEEERRAKPAPDQATAPTPEPAGEAARTAVANGDFEWLRTRHAE
                     GKLTSHVRWHDGGLLTVAVRNNHIDVLEFLLDCGFDPDERVSYGEGDWVAYSQGYPLW
                     HSAALGRHDMATLLLNRGADPNVHVDSSGSAVYSAYSHKQWQMAELLRERGGIVTADI
                     AAIYRETGLVRQMLLDDERGVLPHSGRPSGKPLAEELLRHGAGSGASEIVRMVLERID
                     WTRDDARWFGMLAAPLTFWHHIPWLYAGNKDFGREGYLECFRLLLNVCDVNVTGGFGR
                     TILHEIAAMDDWVAEDEVAAFGRAALEAGARLDRRDDILNSTPLGWACRWGWVQLVRL
                     LLEHGADPEEKDAEAWARPRSWARKMGHDEISQVLG"
     gene            complement(15059..15838)
                     /locus_tag="VFQ79_10420"
     CDS             complement(15059..15838)
                     /locus_tag="VFQ79_10420"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEU0140118.1"
                     /translation="MIDLKELRRLQENWEAPLELLRGSPREVQLTGNGITLWLVVLVL
                     GLGGPAAGVALFAKAEQNAARTLRLAREGSDTEARVTRLSRNRGDNNCRATFLFETGG
                     REYSRTARVPCRVWGKLFQGATLPVRYVASDPRINHLPGFERARTLPNWAGPAIGASL
                     GLAALLLVRVLRYRRRLLETGRPALAIVTRHSRRPSQYGESNKAHYEFATLSGARAQG
                     RYSVGSRKQLPSIGNTLTVIYDRDDPSHNVRYPISLYKLKP"
     gene            complement(15835..16041)
                     /locus_tag="VFQ79_10425"
     CDS             complement(15835..16041)
                     /locus_tag="VFQ79_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140119.1"
                     /translation="MAQRKAFLVRLDPATHDALQRWADAELRSVNAQIEFLLRRALRD
                     AGRLPQPVEESQGGQAPEPRTETP"
     gene            complement(16061..16915)
                     /locus_tag="VFQ79_10430"
     CDS             complement(16061..16915)
                     /locus_tag="VFQ79_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU0140120.1"
                     /translation="MFPEKIIRGVSGWLMVFLLTAVIIGSTVALVRMGNAQWIWVLVI
                     AVAGSCYAGLTVVNPNEARVVVLFGRYLGSVKESGFRWVNPLTVRRKVSLRIRNFESG
                     KLKVNDHDGNPIEIAAVVVWRVVDTAEAVFNVDDYENFVHVQSEAAVRNLATSYCYDA
                     HDAGQLSLRMSAGEINERLKHEVQDRLSKAGIEVIEARLSHLAYAPEIANAMLRRQQA
                     SAVVAARQKIVEGAVGMVEMALEALNAKQVVMLDEERKAAMVSNLLVVLCSDQYAQPV
                     VNAGTLYQ"
     gene            complement(17053..18099)
                     /locus_tag="VFQ79_10435"
     CDS             complement(17053..18099)
                     /locus_tag="VFQ79_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0140121.1"
                     /translation="MLVAEVQPVRAGLKEEAAKRFYRDLTARLALVPGIESVSSSEST
                     PIRGCCWWDPIEVEGYTPAPGEDMKTFFDRVSPDYFRTMGIRVLRGREFTLRDTPSSP
                     PVAIVNESFARRFFPKGDALGRYIALPPNYKLPRMEIVGVVADASFRDLRSPVPYAAY
                     FPLAQDPGARDALQMLVRATGSPSAISAAVRQAVHSFDPGIPVTMRTLADEIDSTLTY
                     ERLLALLSAFFGVVALGLAAIGLYGILSYAVARRTSEIGVRVALGASRGSVLWLVMKQ
                     SIALVLIGMAIGCAAALVLARYVESLLFGLKPADPVTIAIASAGFVAVTALAAWIPAR
                     RATAVDPVQALRYE"
BASE COUNT         3773 a         5631 c         5289 g         3551 t
ORIGIN      
        1 tgccagaatc ttgaagcggg agacttgctg gtgatccctg tttcggcgaa atcgaatcgt
       61 gcggtaggcc ggtcttcccc ggcacgaaaa gtatccgcca cacctaagaa aaaggttgcg
      121 cgaaccacac gtccgacgtc cggcctcaag aagatcagcg ccttgcagaa atccagcacg
      181 cgcgtaagcc ggtagctccg cggcgctacg agctgcttat gacgcccggc cagcgttcaa
      241 cgatgtttat tttttctttc aactgctctt gccttcattt caaaacgagt tattctaggt
      301 ggtgacccgc cggtacagga ggcaacggaa atgtttgatc gcctgttatt ctcgcccgac
      361 gacgacaaag cggacgacta cgaagacgat gagcaaatcg ggctcgattc cgaactcgag
      421 gagtatggtc ccgacgagga cgaggacgag gacgaggaag aagaggatga cgtagtagtc
      481 ctagtcggcc gcgatgagga agacgaggat gaggatgagg aagaagagga aaacgtagcg
      541 cgagccgata gtgaaagtga tgaaggggaa aaggtagcgg tagaggtgcc aattcctgtc
      601 gttgaacctc taacgcctcc cgtcgctgtc aagcaaggtg ggggcgagag acgccggagc
      661 gttaagaaag cagcggtgaa aaaggccgca gggaaagccg ccaccactaa aaaatcggcg
      721 gcgaaagccg gcgcgaaagc agtggccaag aagagctcga aggccacttc taccgcgaag
      781 aagaaagctg caggcaaagc ttcaaccaga aaagcacctg caaagaaagc gcctgtaaag
      841 aagacggcgg cgaaaaaagc ggccggaaag aaagcccccg ccagaaaggc tcccgtaaag
      901 aagggggcgg caaagaaagc tcccgtaaag aagggggcgg caaagaaagc tcccggcaag
      961 aagaccgcgg cgaagaaaaa gactgtaaag aaggcaccag cccgaaagag tgcggcaaaa
     1021 gctggagcag ccaggaaaaa aggcggagcg aagaaggctg gccgtcgccg ctagcaggtt
     1081 tgcctggccg gagatagctt acggggcgtg ccgtattcgc cccgcaacaa gttatttcga
     1141 cacggcagac aagcaacggt catagagttg aagcagggat tcggcatact cttccgcatt
     1201 gccggcgggc ttgtttgcga atcccgctcc ggcggttcct tttccgtatc cggttgttac
     1261 cgagatgaac ttcatcaact cgagtgcgat ctcttccttg ctgcgtgtcg gagattcgga
     1321 tttatcgccg gccatcgatt caggctcctc ctgtttgtag tgaaacccgt agtttagcat
     1381 ttcaatcgat tgcccaatcg cggctcaacc gtaaatgcct gttaacgcgg tacggtttgt
     1441 acgaaaaatg cgcggcggcg cacaggcgca cctcctcgag gccgacgacg gtggatttta
     1501 tgtcgtcaag ttccggaata atccccagca ccggcgcatt ctggtgaacg aatggattgc
     1561 atcggtattc ctgcgctatt tgggaatctt gactccagag acccaaatcg tctctctctc
     1621 ttccgagttc ctgcgggata atcccgaggt cggtttgcag ttcgggacaa agaccgtgcc
     1681 gattcctccc ggctggcatt tcggatcgcg gtaccccggg catccggacc gtaccgcggt
     1741 ctacgatttc cttcccgacg ctttgctgca aaaagtggaa aatttggatc actttgcagg
     1801 cacactcgtt ctcgacaagt ggatgggcaa cgccgatgcg cggcaatcca tttttctgcg
     1861 ggtgcaactg aaagaatgga acctcaacaa aacacacccc gccaggaaag gcttcgttgc
     1921 catcatgatc gaccatggct atgttttcga tggcccgcaa tggaccttct ccgactcccc
     1981 cctgcaaggc ttgtacttcc ggccgctggt ctaccgtgga gtacggtcgg aagaatcctt
     2041 tgaaccctgg ctcagccgcg tcagcacgtt tccggaagaa gttatggacg aagcgtggag
     2101 gcagattcca aaagagtggc tcgaaggaga cgaggatgag ttggaacagc tattgacgaa
     2161 actgctgaga cgaagaaaga atctgccggc ttacctccgg gattccgcca aagcgcgcac
     2221 acatcctttt cccgattggc gctaagccgc tgccgaggag cgcagataag cgatgacgtc
     2281 actggctact cgcgcagccg ttcccggagc catgagaatg ccgttccgat aatggccgta
     2341 tgccaaccaa aggggagtgt cgagatgccg gccaatttgc ggaccgcccg cctcggtgcg
     2401 agggcggaat ccggtccaaa cctttgaagg aacagccgga agtggtagca tacgcctggc
     2461 gcgggcggcg atctgtgcag ctacagtcgc gtccaccgct gagtcgaagc ccacatcttc
     2521 cacggagctt ccggcgatgg tgaagcctcc ggaacgctgc aggatgtacg tctcaccgtg
     2581 ccggagaatg ggatcgagcg agccgggatc gaggttataa gccagcaggt ggcctttgcc
     2641 ggtccacacc ggggaacctc tgcaccctca atcggaatgc tactgctcca ggcacccgcg
     2701 gcaaggacgg cggccggagc gcgcaagaca ccttcgtcgg tccgcacctc aactactccc
     2761 gaatcaaacc ggagaccgag caccggcagt tgctgacgca cttgaacttc gcgtgaaatg
     2821 caggcggcgt gaagcgctcg aaccaggtga tgggggtcca ccaaagcgtc ctccggatag
     2881 aacatcgccc cgatgacact attcaactcc aatccaggca cgagggacga cagcggcggg
     2941 ccggttaccg gatccgtggg cactcccaac aacctctggg tagccgcccg ctccaccaac
     3001 cgcgcccatt cgctctccga ataggcgagt tccgccccac cgcaacgcct gaactcgatg
     3061 gaagtatcgc tctcaacctg caattcgttt acgaagctgg tatacagccg gaaactgtcg
     3121 agtgcaaact gggaccaccc ggtccttcgt ttgatctcgc ctcccggcgc cagcattccg
     3181 gcggcggcgg tactcgcctg ccccccggct gctcccgagt ccagaacggc aacggtaatt
     3241 ttctcctggg ccagccgcca ggcgatcgaa agtccgatca ctcccgcgcc cacaacaata
     3301 acatccggca tgtgaatttg atttcatcgt atcatccggt ctttcacacc catcgatccg
     3361 atacaattgt ctccagatgc acaagttcat cctccacaac ggccaaatcc atgaggcatc
     3421 ttcgaaactt ctgtctccgg gccaggtcgg ggcgctctcc ggatggggag tgttcagcac
     3481 gatccggata ttcgacggcg tgttattcgc tgtcgagcgg cattgggcgc gcatgaagaa
     3541 ggatgccgaa atcatgcggg ttccgtttcc agccgatcct cgccaagtcg aggattcgct
     3601 attggagctg gtggaagcaa atcacgcatc gaatgcggct cttcgcatcg cggttgtgag
     3661 gaatcgcggc ggcatttggg aaggaccggg tatcaaacgg gacttcgatg tcgtggcgtt
     3721 gacaacaaat gtgaggaatt ggggccaggg cgtcaaactc ggcatcgcgg cacaagcccg
     3781 ccatgcccag tcgaggttcg ccggagtgaa gatcctgtcc tgggccgaga acctggtgtt
     3841 atacgaagac gcgcatagcc agggttatga tgaggttgtc ctgctgaacg aacgcggtga
     3901 agtgagcgaa tgcacgtccg cgaacatttt ctgttccaac ggaaatcgtg ttttgacgcc
     3961 gccgctggtt tcaggatgcc tgcccggtgt cacccgggaa gtgctattga ccgaggttcg
     4021 tgtggcaggc atttccattg aggagcgtgt attaacaccg acggatcttg aagccgccga
     4081 cgaagtcttc atcacttcca ctacccgcca actgctgccc gtgctctcta tcgaaggtct
     4141 tgcgatccgg accaaggggc acgcccgcga aaagctacag gctgccttct cggagtacgt
     4201 ggaccggtat gtcgccgccc atcgtggcgc gggcacaaag actcgttagc aggatgctga
     4261 aacggagccg cgcgcacgag caagcggttt tcacccaaaa ggaaatggtt ccaagtgacc
     4321 caagtagcag cttgttctcc agcctgcatg ctaggcctga acgtgacatt cgggaacatc
     4381 gcgcttttct cagtcgctcc acgaaaggtg ttttagcatc tcccgcttca atgcttctga
     4441 gacaacctcg atcatttccc gatagaagct gacttctcga aacgaccttt cactcgccca
     4501 ttccgactat ctcgtttcca aaacagccgc tatggggtcg acgctcccgg cctatttctt
     4561 cgaagtcctg ctgaattcct gcgctacccg ttcggtctga aactcctctc ctcaatgctt
     4621 ttggcatcgg ggagaatcta tattccaaac ccgttgcagc acaacgtgaa tccaacagtc
     4681 ccgaacccat ctacaaactt gaactcccct tcagggtttc cccttccgga tcacagccac
     4741 aaattcgagt gagtcagaaa acttgccgca gtaggttacc cgatcaccct ttgctccccg
     4801 ctggccggta aactataaag tcagccacca acggatcatc gttccaggct cgctgtttcc
     4861 tacccggctc gctgttcctc aaacctcttg gaaccacccc tatgatgggc ccaaatccgt
     4921 tttcagtcaa atgaaatctt tgcatttcga ctggatttca aaagaattta tctgacttta
     4981 tttcagacgt ttagcgggat cgattagtgg attcgttgtg cataaaacag gagcggatca
     5041 atatgtttat tgcggcgccg gcttccggtt gagtttcagg atcattccgg tatcggtggc
     5101 agcccagacg tgatccgggc ccgcggcagc cagaatcacc ccggttgccg tcgcccggta
     5161 gtccacttcc atttcggacc acttggacaa atcgtcacta gacagaatcc ggactctacc
     5221 cggcacgggc aggtgcatga gcgttccggg aggctcgatt gctcccagat aggccgagcc
     5281 gccgggaatc caaacgagat cggtgacggc gcggtccttt tgacggaata cgcggtcgct
     5341 tttcccggta tggaggtcga gccggtatac ttccgccgga tattgaaagg acccttggaa
     5401 ctgaaacagc gccagcccga agccatcgcg acggagatca agggaagtaa ccgtcccgaa
     5461 aagagaggaa ttggaagctt gccagctttt gcccgcgtca gcggtttgca gtagcagcgt
     5521 caaggtgggc cattccggac gtttacccct ctcgggatcg atccattccg gcaattgggg
     5581 ctcatcccgc cgctgaggcc tgctggaccc cacgatgatg cccacctgcg gcgtcgcaaa
     5641 atcgatgcac cggtaggcgc tgtccttcgg gttgcctttg gggatttcag cagccgcaac
     5701 ccccgtccac cgccggccac cgtcgtgggt ttcgagcacc tgctttttcc cacctatcgc
     5761 ccatccatgc tccggagtag tgaaaaacac ctcggcgatg tcccgtatcc ctttgagttt
     5821 cttccagtcc cggcccgctt cctgtgtttg ccagattccc ttctcggtca ccatccagcc
     5881 cagggagtca ttcagaaaga agagagaaat gggggtctct ctgagacgct gcatctccca
     5941 ggtgcggccg ccatccccgg tcaggacaac gactggccgc tcgcgcccct tctcggtcag
     6001 cgtcccaatt gcgatacctc tctgctccga cggaaaaacg agatcctgga acacaagttg
     6061 cgaatccagc ttgtcgtaga aatactgcag ttcccacttc cctgcgcctt ggccgaggct
     6121 ggcaagcaac agcgtgagaa ggatggacct caccgcaatc tcctgcttag gcttgcctct
     6181 cgggcgccgc cggacccgag gagagcacca gttcgacatg attcgccgcc tcaaccgcag
     6241 taggctccat tctggccagg accagtagac aaccgacctg ttcaccaacc gtagtaagca
     6301 actccatctc gcttccgctg tgagcatgag gaagtttgtg ctgcacgttg atgacaccca
     6361 ccacgcgatt gcgagcaatc accggcgccg ataaaaaagc ctcgtatgtg tcttccggga
     6421 ggtcgcggaa atacttaaac cgggggtctt tatagacttc tctggaaata gcgagcaatc
     6481 tccgctcgcg cgcgacccac ccggtcaaac cctcgctcat cttcagccga acattgccga
     6541 ccgcagccga atgagccgta ttcgaggcgc agagtaccag ttcgtcgttg tcgatgagat
     6601 aaaccagaca ggaatcgcac tgtgtgaact cgaccaccag ggaaacgatg ccctggagga
     6661 cttcctgcaa gctcgactca cgcaccataa accggctgat cttctggaaa agacggagtt
     6721 gttgttcggt acgctcgagg cggaattcca gatcctttga cttgggcacg tgcttattat
     6781 cccagaaaag gctgcatgcc cggtttccac gactcaaaag cctttcacgc aagaactccc
     6841 ggcagtccgc tatactgaag gagactggga gctcatgaat ggcccacccc cggcgatcac
     6901 tgttttacct tccccttacc ctgattttgt tctccctttt gggaggcctg tttggtccgg
     6961 gtctcgcgcc cacggcggcg gcgacctcgg acgacgacat tcgtcagagt gttgaaacat
     7021 tcaccaaggt tctttccgtg ttggaggaaa acacggcgga gcctatagat acggacaagg
     7081 cgatctataa cggcgccatt tcgagcatgc ttcgaacact cgatccgcac tccagcttct
     7141 tcgatccgaa gcttttccag gttctacagg aagaacaacg gggccagtat ttcggcgtcg
     7201 gaatgctggt aggcgctcgt gacggcgggg tagttgtcgt gtatcctttc tccaattcgc
     7261 cggcattcca gggcggcatt catcccggag acatcattat tgccgtcgac ggcaagccca
     7321 ctgtgaaggc gagcacttcg gaagtcgcgg atttgctgaa gggtcccagg ggcaccaccg
     7381 ttcaggtcac cgttcggcgc cgcggcgcaa ccgggccgct acgtttcacg ctcgtgcgga
     7441 acagtattcc gcgggcgacg gtgcagaacg ccttccgcat ccggcccaaa atcgggtatg
     7501 tccgtatcga atcttttaac gagaatacca gccgggagtt cgaagagagt ctgcgcgagc
     7561 tggacgaatc gacactggaa ggcatggtgc tggacatgcg gagtaatccc ggcgggttgc
     7621 tgcgggaagg tgtcgccatt gcggaccgtt ttctccgcaa agggcagttg atcgtctccc
     7681 accgcggcag ggcgtcggcc gagcgaccct atgtcgcgaa gcggggcaac gacggccgcg
     7741 aatacccgct cgtcgtgctg gtggaccgca cttccgcctc ggctgcggag attgtagcgg
     7801 gcgctctgca agaccacgac cgcgcctgga ttgtaggcga acctaccttc gggaaggggt
     7861 tggtgcaatc gcagtatccg ctatccgcca ataccgcgct cctgctcacg accgcaaagt
     7921 actacacgcc gagcggacgg ctgattcagc gggattactc gaaccgttcg ttctttgaat
     7981 actacgcgca taaagatctg acagagcggg atccgaaaga catgaaggcc accgacagcg
     8041 gccggccggt atacgggggc ggtggaatca cacccgacga agagtacacg cgtgaaaagc
     8101 tgaatgcatt tcagaccgcg ctgctgcgtc gggacgcgtt cgccaattac acagcccagt
     8161 atttcagcag ccgcaaaaca gagttgcctg ccgactggac tcccgggcgg gagatcgagg
     8221 acgacttcca ccagttcctg ctgaaggagg gcgtttcgtt taccgaagcg gatttcgcgg
     8281 ccaatcatga atggatccgg cagcgcctgc acgtcgatat gtaccttacc gcaaagggaa
     8341 aggattccgc ggacaggcta tcgatcgaga tcgaccagca ggtcgcgcag gcgatcgatt
     8401 ccatgccgaa ggccaaggcg ctgatcgatg ccgcaaaggg catcctggcg aaacgcgcgg
     8461 aattacagta acctatccga taccgcttgc gcgcgcggct ccgtttacgc ttttgctttt
     8521 tccggtggag gtacagggcc ccggctgaag agggatttct ccggcgcctg gtacttcacg
     8581 tccaggtgtg gagtctccat cttccggtgt tcgtaggacg aatccatcag ggtcgccagc
     8641 gctcccgtag taagcaacgt ccgctcgaca gggtaggaag gctttccggt aagcaccagt
     8701 tcttcgatgt agtacgtcag cgcgctgaaa tggctgaacg gccggccggg ttgcagccag
     8761 atctccgttg caagcggctc cttgcgttca ggcaagtttg tagcaaagac acactgatcc
     8821 agatacccgt tcagcatata caccgccgcc tccaatccat cgcgataccg gagcacgaat
     8881 acagcagggt tcttcgtgac ctcgcgcatg ggtccggctt tgcgggtttc gctccgcgcg
     8941 acagccgatt ccagcaaagg accggcccag gcgttttctg aagtccactt ccacacgtca
     9001 ggcccttcga tcgcatggac ctgcgatatg ccggtttccc cgccttgccg ccgctctacc
     9061 atgcactgaa gcgcctcgag cgcgtggaag ccataggctt ccttaccgcc atagaacgcg
     9121 gcaacggcat gacgaacagg ggttcccttc ttcaactcca cgggcggatg ccgccaagtc
     9181 accggcagcg aagagccggc catcatcgga aagccgagtt cgcgcgattg atcgtacatc
     9241 catttggctt tcttccattc caccgacagg tgcttgtccg taaataccgg cacactgcgg
     9301 ccgctcgagc ggaatacttc gacgatcttg ctgaagagct cgtagcgcgg gtataacttc
     9361 tggccttttt cgttcgttgg ataattgcca tgctcgccga tcagaacgac gccgtccacg
     9421 gccagctttc ccgtcccgag tgtcagtgct tcggcgatgg tgggtttaat ggcgacggcg
     9481 tgtttcgccg ccatggcgcg gctcatatca ttcgcaggca cctggtccgt gtacatcgag
     9541 accatcttca cgcggggctg ccggcgcctg ccgtcgaaat cgtacccttc gagcagacgt
     9601 cctgcgatca catcggcatg cgaattgtgc cggtattcgg tgatgatggc agctaccgtt
     9661 ttgggtgctg tctgaaaggc ggccagcgga gccagaccgg gcacggccag aaattcacgg
     9721 cgtctgatca ttgctcgatt ttacacgctg gatgacgccg gggccgctcc ctcgcggtca
     9781 cggctcggaa acggagccgc gcgcgtcagc aagcggcatc agatatgtca ctttacggca
     9841 tcaggtcgcg gaaagacagt ccaaagcgaa agccccacaa gtgcagcctg tcggagtagt
     9901 ggtcgcggaa ctcgacgcgc acagccacgc tcggctggaa ccagtagttg aacccgccgc
     9961 cgtagttcac catgttacgg gttccgctcc ggaatgcgag cgaatatccg gcggttgcaa
    10021 aggggtccca cttgtcatcg cgggacggca ggaaatgata ggttccattg aacgacgcga
    10081 tgcctatgac cgaatcgaca tagtccctgg gcagacccca tgcgccggct tccaggccaa
    10141 gagccacacc cctcttaagg gcgatttcac caccgccgcc catgtgcagt gagaactgcg
    10201 tgcggccgcc gcttgtgccc cctcccggcg caacgaacca atagccgtga gaacgctgcg
    10261 ccgcagcggg aacagccatt ccggtgaggc acagaacggc cgccggcatc catttccaat
    10321 tcattcaatc tcccgatgtg gacgcccgct tccgcgcgcg ggtccgacaa acacaagacg
    10381 caggcagcgg cgggaaagtc ggaatccgct gaaattcacc aatcgagcac cgcgccgtcg
    10441 ggcgcgcgcg ccgcgagcga ttgaatcacc tcctgttcga acgggtgcag ccgtgcggcg
    10501 ttctcatcca cctccacacc cagcccgggc acatccgatg gcagccaata tccgtctttg
    10561 ctcgtgagcg aatggcgcac gacgtcccag cgccacggca cgtccgagag catgtcttcc
    10621 tgtatcagaa aattcggtgt gctcaaggcg aaatgcagag cggccgcgtt cgcaacaggg
    10681 cccaggggat tgtgcggcgc cactccgacg taatacgttt cggcggcggc ggcgatcttc
    10741 ttcgcttccc acaagcctcc gcaatggcag aggtcgggct ggatgatgtg acaagcctgc
    10801 ttttcgaaca aggtccggaa ctcgaaccgg gtcaccagtc gctctcccgt ggcaatgggc
    10861 accgggctcg agcgcttcac ctccgccatg gcgtcgacgt tttcgggcgg aaccggctcc
    10921 tcgacgaaga acggacggta gggcgccagc acgcggcaga actcgagtgc gttggccgga
    10981 aagtgcctcc catggcagtc caccatgatg tcgatgtcgt cccccagcgc gcggcggaga
    11041 gcttccatcc ggctttccgc atgacggtag agtgcggcgc cgttcaggct ttcggttggt
    11101 ggtgtgagga ggaccttgac cgccgtgtag ccgcgcgcgg ccacttccag cgcaaggtcc
    11161 accatggcgg aagcgtcccc ggaatgctgc gattcgtaaa cggccttcat ttcgccgccg
    11221 cccaggtgtg tatacatcct cacccggtcg cggaccgccc ctccgagcag gcgatacaca
    11281 ggcatcccgg taatcttgcc gagaatgtcc caaagcgcct gctcgatccc cgagatggcc
    11341 gacatgccga taacacccgg ccggaaaaac gactgccggt acatcttctg gtagagatgc
    11401 tcaatgcgtg ttgggtcttc accgagaatc atcggcgcca gatcctcgac ggcgcccgcc
    11461 acactgcggg tcttccattc gagcgacgcc tcaccccatc cccagaggcc gtcctggtcc
    11521 gtctccactt tcacgaacac ccaattgcgc atccgggcat ttaccacaac ggttttgact
    11581 tgcgttatct tcatcagagc tcctcgtggc catcggccgc ggctgctatc atgatgcact
    11641 gaacgggaat tccgtggatc gcctttccga aaccacctac gacgaagttg cgtatccgac
    11701 atttccgcgg tttcagaccc atccggcgca ccttcaaacc atggcctttc taggcggcct
    11761 gaaaagcgct cctcccggcc gttgccgcgt tctggaactg ggttgcggcg atgggggcaa
    11821 cctgctttcc atggcggtgg cccttccgga gagccggttt gtgggagtgg atctggccgg
    11881 cacggccatt gaacgcggca actccacggt gacggcgcta ggacttgcca acatgcgcct
    11941 gatgcaggcg gacctgctga acatcggtgc gtctattgga gagttcgatt acatcatcgc
    12001 gcacggcctc tacgcgtggg tccccgatcc ggtgcgagag aagctgctcg ctatttgcgc
    12061 ctctcaactg gcgccggacg gaattgcctt catcagtttc aattcgttcc ccggctgcca
    12121 tttgcggctg gcattgcgcg agatgctgtt gtttcacata cagcacatcc ggaaccggca
    12181 acagcgtctg gcagaagcgc gatctcttct aaaagcgctg agcagcgcgc tggtggcaga
    12241 cccgcgcgga gaggccatcc gcaaggaagt ggaaaggctg atcgatctgg aggacggata
    12301 cctgtttcac gacgatctgg cggaagtgta ccgtccggtt tacttccatg aattcgccgc
    12361 gcacgcgcag cggcacgggt tgcggtatct ttgcgatgcg cggtactggc acatggagga
    12421 atcgcctctt accgatgtcg cgggcgcact ctcggagacg gcgggcgaag atcgtgattt
    12481 gcgcgcccag tatgtcgatt ttttcaaagt gcgtccattc cgggaggcgc tcctgtgcaa
    12541 aggtgatcgg acgcccgatt tccgtgcgct gtccgggcgg ttccagaatc tctttgttgc
    12601 ctcgtcttcg gcgcccgtag gcgagcccga tgcagaaggc ggcctgcgct ttcagatcgc
    12661 cgaaggccga agcctgtcca ccaaccaccc gatcgccaaa gcggcactgg cctacctcgg
    12721 ccgggtttgg ccgcgatggg cgcggttccc ggatctgctg gatgaaatcc tcgctccgga
    12781 tgtggatgaa gatgcgagac ggtcgtccgg ggaagctcta tcggagatgc ttctgcgtct
    12841 ctcggcagtg cgctttgtgc aagccctgac gacgccaccc cggctcgctt cgcagctttc
    12901 ggagcgtccg gttgcgagcc ccctggtgcg atatcagctt tcgaccggca gcgccaaagt
    12961 ctccagcctg cgccacacca cggtggagtt gagcgatccg ctgtcacggc atacggtgcg
    13021 gctgctcgat ggaacacaca atcgcgcgga attgctggag gaactcgtcg cactcgtcaa
    13081 actgggcgtc gcgaaactgg aaatcaacaa ggagcagatc accgatccgg cgcgcgtgcc
    13141 ggatcttctc gcgcggagcc tcgaggggaa tctcgagaag ctgatgcggc tcggtctgct
    13201 ttccgcctga gcagaagagc gaccgcgccc ccgtccgcta gccgagcacc tgcgaaattt
    13261 cgtcgtgccc catttttcgc gcccaggagc gcgggcgtgc ccatgcttcg gcgtctttct
    13321 cctccgggtc agctccgtgc tccagcagca gcctcacaag ctggacccac ccccaacggc
    13381 atgcccaacc gaggggagtg ctgttcagga tgtcgtcgcg ccggtccagg cgcgcgccgg
    13441 cctccagcgc cgcacggcca aacgccgcga cttcgtcttc ggcgacccaa tcgtccatcg
    13501 ccgcaatctc atgcaggatg gtccgcccga agccaccggt gacgttgaca tcgcacacat
    13561 tcagaaggag acggaaacac tccagataac cttcgcggcc gaaatccttg ttgcccgcgt
    13621 agagccatgg aatgtggtgc cagaaagtca acggcgccgc cagcatgccg aaccagcgcg
    13681 catcatcgcg ggtccagtcg atgcgctcca gcaccatacg aacgatctcg ctggcgcccg
    13741 agcccgcgcc gtggcggagc aactcttccg caagcggctt gccggatggc ctgccgctat
    13801 gtggcaagac gccgcgctca tcgtcgagca gcatctgccg gacaaggccg gtctcccgat
    13861 agatggcagc gatatcggcc gtcacaatac ccccacgctc gcgcagcaac tctgccattt
    13921 gccactgttt gtggctatac gcgctgtaca cagccgaccc gctggagtcg acatgtacgt
    13981 tcgggtccgc gccacgattg agcagcaacg tcgccatatc atgccgtccc aacgccgcgg
    14041 aatgccacaa cggataacct tgcgaatacg cgacccagtc tccttcgccg taggagacgc
    14101 gctcatccgg atcaaagccg cagtcgagca gaaactcgag cacgtcgata tggttgttcc
    14161 gcacggcgac cgtgagcagg ccgccgtcgt gccagcgaac gtgactcgtg agcttccctt
    14221 ccgcatgacg ggtgcgcagc cactcgaaat caccgttcgc cacagccgta cgggcggcct
    14281 cgcctgcggg ctccggtgtt ggagccgtcg cctgatcggg tgcgggttta gctctgcgtt
    14341 cctcttcctc ctcgatgatc gcgacgatct cgtcgtaatc ccgctcccgg gccaagatcc
    14401 acgccgacgt ggcatcgcga tgcggatgga tgcccgcccg tgcgttggcg cctttctgca
    14461 tgagcaatcg cgtaatttcc gggtgccggt gcattacggc gtagtggatc gcgcggtgtt
    14521 cgtcgccgta ggaaaccgtc atgttggcaa gctcgggccg ggtcttcagc atcgttcgta
    14581 cacgcccggc atggccggcg cgcacggctt ccacgaatcg acggatcgtt acgccgtcaa
    14641 cgtaagcctt cagcttcggc cagctatcga atccatagct tcgcgcgaga accagttgcg
    14701 catcgtgtaa agcgaagacc gccgcttgtg caccgtcgta atgtgcgttg acctcgccgg
    14761 cggccgctgg gtcgcctgcc ttgaaggccc tgagcaattg cttcgcttgt cgtttgagtt
    14821 gttcgaggtc cggatgctcg cgcatccggc gagtgggtag cttctgtttc acatgagctc
    14881 ctttccacaa cgcccggtgt ccgctagctc gggcggaaaa gagttcactc aaccaagtgg
    14941 ttgcaagcca aaatccaggc gggacgcagt ccctttccgc ggacacggag gcgtcctgag
    15001 cacgccacaa ccaaaataac accgatgcga agactcccgt tatcgaagaa ggtgggggtc
    15061 agggtttcag cttgtagagc gagatgggat agcgcacgtt gtggcttggg tcgtcgcgat
    15121 cgtagatcac ggtgagcgtg tttccgatgg acggcagttg cttcctgctc cccacgctat
    15181 accggccttg cgcgcgcgcc ccgctcaagg tcgcaaattc gtaatgagct ttgttggatt
    15241 ccccgtactg ggaggggcgc ctactatggc gagtcacgat ggcgagcgcc gggcgtcctg
    15301 tttcgagcag ccggcgccgg taacgcagca cacgcaccag cagcagggcc gccaggccaa
    15361 gcgatgcccc gatcgccgga cctgcccaat tgggtagcgt acgggctcgc tcgaacccgg
    15421 gcaggtggtt gatccgcggg tccgacgcca cgtagcggac cggaagagta gcaccctgaa
    15481 agagctttcc ccacacgcgg cacggcacac gggcggttcg cgagtactca cggccgccgg
    15541 tctcgaacag aaaggttgcg cgacagttgt tgtctccgcg gttccgcgac aggcgcgtga
    15601 cccgggcctc tgtatcggat ccctcccgcg ccagccggag ggtgcgggcg gcattctgtt
    15661 ccgcttttgc gaagagagca accccggccg caggtcctcc cagccccagc accagcacca
    15721 ccagccacaa cgtgatcccg tttccggtca actgcacttc gcgcggggat ccccgcagca
    15781 actcgagcgg cgcctcccag ttctcctgca accgccgcag ctctttcaaa tcgatcatgg
    15841 cgtctccgta cgcggttcgg gcgcctgccc gccctgcgat tcctccaccg gctgcggcaa
    15901 gcgcccagca tcgcgcagtg cccggcgcag cagaaactcg atttgcgcgt tcacgctgcg
    15961 caactctgcg tcagcccagc gttgcaacgc gtcgtgcgta gccggatcga gcctcacgag
    16021 aaatgccttg cgctgcgcca tacgagcgac cgccgtcctc ctactgatag agcgtacccg
    16081 cgttgaccac cggctgggca tactggtcgc tgcacagtac gacaagcagg ttgctcacca
    16141 tggcggcctt acgttcttcg tccagcatga ccacctgttt cgcgttcaga gcttccaggg
    16201 ccatctccac cattcccacg gcgccttcca cgatcttctg ccgggccgct acgacggcgc
    16261 tcgcctgctg ccggcgcagc atggcgttgg cgatctccgg cgcgtaggca agatggctga
    16321 ggcgcgcctc aatcacctcg atgccggctt tcgaaaggcg atcctgcact tcgtgcttca
    16381 gccgctcgtt gatttcgccc gcggacatgc gcaacgaaag ctgaccggcg tcatgcgcgt
    16441 cgtagcagta ggaggtagcc aggttgcgca ccgccgcctc gctctgtacg tgcacgaaat
    16501 tttcgtaatc atcgacattg aacacggctt cggcggtgtc gacgacccgc cacaccacca
    16561 ccgccgcaat ctcgatcggg ttgccgtcat ggtcgtttac cttcagcttg ccgctctcga
    16621 aattgcggat ccggagtgag accttccggc gaaccgtcag cggattcacc cagcggaagc
    16681 cggactcctt gacgctgccc aggtaccgcc cgaagagcac tacgaccctt gcctcgttag
    16741 gattgaccac cgtcagaccg gcgtagcagg aacccgccac ggcgatgacc agcacccaga
    16801 tccactgggc gttgcccatg cgcaccaacg caacggtgga gcctatgatt actgcggtga
    16861 gcaggaagac catcagccag ccggaaacgc cgcggatgat cttttcggga aacatatact
    16921 tttcctcctt aaaatcagaa tgatatcact taggtatcaa aatgcaagta aaatcaaaat
    16981 ctcagaggta agggcgtgtg gggaaaacag tttgcagtgg tcccgggctg tataccggca
    17041 tcgccggatg gcttactcgt atctcagggc ttgaactgga tcgacagcgg tcgcgcggcg
    17101 tgcgggaatc cacgccgcga gcgccgtcac ggcaacgaag cccgccgagg cgatcgcgat
    17161 cgtgaccggg tccgccggct tcaagccaaa tagcaggctt tctacatacc gggctaagac
    17221 caatgcggcg gcacaaccta tcgccatccc gatcagcacc agtgcgatgc tctgcttcat
    17281 gaccagccag aggacgcttc cacgggaagc ccccaacgca acgcgtacgc cgatttcgct
    17341 cgtgcggcgg gccaccgcat aagagaggat tccgtacagt ccaatcgcgg ccagtcccag
    17401 cgcaaccacc ccgaagaaag cactcaaaag cgccaacagg cgctcgtaag tcagggtgct
    17461 atcgatttcg tcagcgagtg tccgcatcgt caccggaatg ccgggatcga acgagtgaac
    17521 tgcctggcgc accgccgccg agatcgcgga tggggatcca gtggcgcgca cgagcatctg
    17581 aagagcatcg cgagcacccg ggtcctgggc cagcgggaag tacgccgcgt acgggacggg
    17641 agaacgcaag tcgcggaacg aggcgtccgc caccacgcca acgatttcca tgcgcggaag
    17701 tttatagttg ggtggaagtg caatgtaccg gccaagcgcg tctcctttgg gaaagaacct
    17761 gcgtgcaaag ctttcgttaa cgatcgccac gggaggcgac gagggcgtgt cacgaagcgt
    17821 aaactccctg cctcgcagaa cacgaatgcc catggtcctg aaataatccg gcgagacacg
    17881 gtcaaagaac gtcttcatgt cttcgcccgg cgccggtgta tagccttcca cctcgatcgg
    17941 gtcccaccag cagcagcctc gaatcggcgt ggattcggaa gaactgacgg actcgatgcc
    18001 cggaacgaga gccaggcggg ccgtgaggtc gcgatagaaa cgcttggcgg cctcttcctt
    18061 gaggccggcg cgaacaggct gcacttcggc caccagcacg cgcccatacc ggctcgaatt
    18121 ccgccgtcgc ctgttgccgc ggcaaacccc cgcgaagccg gcccatcaaa ggaagccatg
    18181 acgcgttgcg ccgtaacagt atttcgctgc cgggtgcgag cagccgttcg gcggataacg
    18241 gtat
//
LOCUS       DASTMK010000237         9695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_12238, whole genome shotgun sequence.
ACCESSION   DASTMK010000237 DASTMK010000000
VERSION     DASTMK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12238"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="VFQ79_10440"
     CDS             <1..1665
                     /locus_tag="VFQ79_10440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0140122.1"
                     /translation="DKVPLGNLARQSLEEIWQGEAYQRFRKRYHQAAVPECRSCPWKM
                     AYRPSPVQSEILGARGRSAQLLQGWHEPSDETHIWSSQRAKAILAPQVTSGVLHVSGM
                     LPPDTKNGANTLVIRCNEREIGTVVNPGQGVLSFQQDFPVQDRRAEVWLLEFLTRGVY
                     RPSWNGSGTDQRDLGFALTTLASRPSVDPRHVERHRKQLRPLVRWIRAVDLLGALIGR
                     CVRRRKPIAIPATLSPGLSILIPEWDNAGELSECLEGVQRALKHWTEPVEVMVVVNGS
                     PRSSYRSLQASHPTVAWQFHDRPLGFSGAIHAGLKAARFEWVYLLNSDVVLDPYALKV
                     AGGCRDSSLFAIASQVVLKDATRFRDETNWTTLFLEDGLVTIHDLIPRSEVPVEGFYA
                     GGGAALFQTRLLRSFLDATVYAPFYWEDVEWGWRARKSGYRVLFCPASIAHHTRRSTI
                     ERHYSPAEVESVLQRNRLMFQLRNLSGAGSVRRAVEEIARSPHSMVDYFGRPWTLWNI
                     ALGRLWNRAAAIGDEEILNPWKACPHPGSRPRAETAILSASCRQSL"
     gene            1647..2429
                     /gene="thiD"
                     /locus_tag="VFQ79_10445"
     CDS             1647..2429
                     /gene="thiD"
                     /locus_tag="VFQ79_10445"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU0140123.1"
                     /translation="MQAVALTIAGSDPSGGAGIQADLKTFHQFGVYGEAVITLLTVQN
                     TLRVSRVETVAPDLVRQQIEAVLQDIPPGAAKTGALGNRDIVEAVAALAADFDFPLVV
                     DPVMISKHGARLMGEDARDSIERLLLPVSFLITPNLPEAAVLSGIAVENVDDMREAAR
                     RLCGKGARSVLVKGGHLAESAMDILLHEGAWYEFPAPRIETPNTHGTGCTYSAAITAG
                     LASGRKLPDAVARAKRYIAAAIQNSPGLGHGTGPVQHQASPD"
     gene            2474..4243
                     /locus_tag="VFQ79_10450"
     CDS             2474..4243
                     /locus_tag="VFQ79_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140124.1"
                     /translation="MKFLVATIGMLAAVLTAYGKIPEQDSRNTDIPHTDTHFRARSYT
                     SLEEWQQRAPQLRKQVLSAAGLLPLPERNPLRYEIFGRIENKDYSIEKVFIETMPGYY
                     LAGNLFRPLGRTGKFPGILAPHGHATYGRLENGPLFSVPARGINKARQGYVVFAYDMV
                     GYNDTIQTPHVFGGEREQLWSFGPLGLQTWNSIRALDFLQALPDVNPNEIGVTGESGG
                     GTQTFLVCAVDDRIKYSAPVNMVSLIMQGGSPCENAPNLRVATNNVEIASLMAPKPML
                     MVSATGDWTKNTPTEEYPAVKAIYSLYGKPENVDTIQIDAPHNYNKPSREAVYRFFAK
                     HILHDPNWASYSERNIRTEKLQDLMVFHARPLPDNALKYDQIAAQWIERAQRQNASIT
                     GRSAMRERLMYSLMAEQPQAVEHEISGERILLGRQGKGDRVPGIWIPGGKQALLVVHP
                     EGAAAARNSKQASDAISKKQSVLLIDAFQTGSAAAPRDRSKRYFLTFNKSDDANRVQD
                     ILTALAFLKDQGASGIRLAGVGKAAIWATFAAALSDAAVTLDADLGSFQGSDEDFIKD
                     FFVPGIQRAGGLKAALALAGSSR"
     gene            4292..5368
                     /locus_tag="VFQ79_10455"
     CDS             4292..5368
                     /locus_tag="VFQ79_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0140125.1"
                     /translation="MTLDAGGTNFVFSAMRSNRPVTEPFALPSYGGDLEQSLNTILEG
                     FDRVREQAPSPPVAISFAFPGPADYENGIIAGPRNLPAYRNVALGPMLEDTFGLPVFL
                     NNDGDLFTYGESMAGLLPFVNRMLEHAGSPKRFHNLLGVTIGTGLGGGIVRDGELFKG
                     DNSIAGEMWLLRSKADPSRNAEEHVSIRAVRRAYAQLAHIGLSEAPEPKEICDIAEGR
                     REGDRAAAVEAFRRLGEAAGDAVAQAVTLVDGLVVIGGGVSGAHPLFLPALVDEMNAT
                     FLTPDGTRFRRLVQRTFNLEDSAQLKQFIQGEPQELCVPGGTRRVRFDAQQRTGAGIS
                     RLGTSQAIAIGAYAFALKQLDSGG"
     gene            5949..7043
                     /locus_tag="VFQ79_10460"
     CDS             5949..7043
                     /locus_tag="VFQ79_10460"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0140126.1"
                     /translation="MKRELEPVRLRARDTGLCKRALITGGMGFIGSNLALRLVELGVD
                     VTVVDSRVVDCGSNPWNLSGAAREIRVIERDISEAAGLQREISESDVIFNLASEISHI
                     HSMQYPQRDCALNAVAQLRFVQACERFNPGVRIVYASTRQIYGIPHYLPVDEGHPVHP
                     VDYNGVHKYAAMMYHLIAARRGRLDAVVLCLSNVYGPRMAINIPCQGVLGNFVRRLMQ
                     GTRLEVFGNGRQLRDPVYVDDVVDAFLRAAGVRRPRSRTYNLGGPDALALRKIAQIAS
                     SAAGVAAPVVHPFPKERKSFDIGSYYTDSSLILRELGWKPRVKFEEGIARTLEYYRKE
                     LSHYLEPDGEHTGCKLTEQKSARGMVAAAL"
     gene            7040..7792
                     /locus_tag="VFQ79_10465"
     CDS             7040..7792
                     /locus_tag="VFQ79_10465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0140127.1"
                     /translation="MMNRAEFANIARAERQSWWYAGMREILLRTLDPWLAGRHIERVL
                     EAGCGTGYFAHTMQTERKWPIHPVDLDAEAVRRARTMGLERVTQADVAALPFRDSAFD
                     LVLSLDVLVHFARGEEYRPLSELKRVLAPGGLLALRVAALDVLRSRHSQFVWERQRFT
                     RSRLLKAVRRCGIRVLRCTYANSLLMPVALTKFRLWEPILRKPPSSGVAPVSVWLDRL
                     LRVPLALEAKWLGSGIDLPLGQSLLLIGEKMP"
     gene            7789..8535
                     /locus_tag="VFQ79_10470"
     CDS             7789..8535
                     /locus_tag="VFQ79_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0140128.1"
                     /translation="MTEAGIGSLTVFFPAYNDAPSLPQLLQRTFAVLREHATDYEAIV
                     VNDGSRDNTEAVLRELQACYAPRLRVITHPHNRGYGGALRSGFAAATKEFVFYTDGDG
                     QYDPTELPRLLERMKPGIGLVNGYKLERHDPLHRIWIGRVYNRLARALFRIRISDIDC
                     DFRLIRRSLLEGMTLTSTSGTICVELVRKIELSGCGVVEVGVHHYPRLHGRSQFFRTR
                     SLLTTFGQLLRLWIRLVAVPAVFGKDRLRS"
     gene            8544..9629
                     /locus_tag="VFQ79_10475"
     CDS             8544..9629
                     /locus_tag="VFQ79_10475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0140129.1"
                     /translation="MVNLRPLLEETRPQWTANLSRLFERMQFILGEEVACFEQELARS
                     FGSRFAVATGTGTAAIELSLRAAEIEGREVLLPALTSLFTAQAVLAVRCIPRFADIHP
                     GTLLLDVADVERRITPATAAIIPVHLYGQPCPMRALRQIARRAGLTLVQDGCQAHGAR
                     HEGVALTRFSSCVAYSFYPTKNLGCLGDGGAVLTDRSSVAERLRLLRDGGRRNGQVSR
                     LPAINSRLDEMQACYLRAFLPRLAEWNLERARIASLYDEALSDCPGIESVRRSTDSVN
                     HLYVIRARRRDRLRVHLAARGISTAVHYPVPLHLQPAFREFGPARGQLPNAEKACREI
                     VSLPVWPKMPEDAVLRVADNVREFYKK"
BASE COUNT         1837 a         2956 c         3017 g         1885 t
ORIGIN      
        1 gacaaggtcc cgttgggaaa tctcgcgcgg cagtcactcg aagagatctg gcagggagaa
       61 gcctaccagc ggtttcggaa acggtatcac caggctgcgg ttccggaatg ccgctcgtgc
      121 ccgtggaaaa tggcttaccg cccgtctcct gtgcagagtg agatcctcgg cgcgcgcggg
      181 cgcagcgcgc aactgcttca gggatggcac gagccttcgg acgaaaccca tatctggagc
      241 agccagcgag cgaaggcgat cctggcgccc caagtgacat caggggttct gcacgtatcc
      301 ggcatgctgc cgccggatac gaaaaacggc gccaacacgc tggtcattcg ctgcaacgaa
      361 agggaaatcg gaacggtcgt gaacccgggg caaggcgtgc tgtctttcca gcaggacttt
      421 cccgttcagg accgccgggc ggaggtttgg ctacttgagt ttcttacccg cggcgtgtat
      481 cgtccaagct ggaacggctc aggtacggat cagcgcgatc tgggatttgc actaacgacg
      541 cttgcctcca gaccgtctgt agatcctcgc catgtggagc gccacaggaa acaactccgc
      601 cccttggttc gctggatccg ggccgttgac ctgctgggtg ctctcatcgg ccggtgcgta
      661 cggcggagaa aaccgattgc aattcctgcg acgttgtctc cgggactctc catcctgatt
      721 ccggagtggg acaacgccgg ggaattatcg gaatgtctcg aaggcgtgca gcgcgccctg
      781 aagcattgga cggagccggt tgaggtgatg gtcgttgtga acggttctcc acgctccagc
      841 taccgttctc ttcaggcttc tcatcccacg gtcgcatggc agtttcacga tcgtccttta
      901 ggcttttcag gcgcgatcca cgctggacta aaggctgcgc gattcgagtg ggtgtactta
      961 ttgaacagcg acgttgtact cgatccctat gctctgaaag tcgcaggtgg ttgccgggat
     1021 tcctcgctgt tcgcgatcgc ctcgcaggtg gtattgaaag atgccacccg tttccgggat
     1081 gaaacgaact ggacgacgct gtttttggag gacggccttg taacgattca tgacctgatc
     1141 ccccgatccg aggtgccggt cgaaggcttc tacgctggtg gaggcgccgc gctgttccag
     1201 acgcgactgc tccgctcctt cctggacgcc actgtttatg cgccgttcta ttgggaggat
     1261 gtggaatggg gctggcgcgc gcggaagtcc ggctatcggg tgttgttctg tccggcttcg
     1321 atagcgcatc acacccgccg gtccacgatt gagcgccatt attcacccgc ggaggtcgag
     1381 tccgtgctac agcggaaccg gctcatgttc cagcttcgca atctctcggg agcaggttcg
     1441 gtaaggcgtg ccgttgagga gatcgcgcga tctccccact cgatggtgga ctattttggc
     1501 cgtccctgga cactttggaa cattgcgctg gggcgcctgt ggaatcgtgc ggcggcaatt
     1561 ggcgacgagg aaattctgaa cccttggaaa gcgtgcccgc accccggcag ccggccgcgc
     1621 gcggaaaccg ctatcctgag tgcttcatgc aggcagtcgc tctgaccatc gccggttccg
     1681 atcccagcgg cggtgcggga attcaggcgg atctcaagac cttccatcag ttcggcgtct
     1741 acggcgaggc cgttatcacg ctgctgaccg tgcagaatac gctacgggtg agcagagtgg
     1801 aaaccgtcgc tcccgatctc gtccggcaac agattgaggc ggtgctccag gacatcccgc
     1861 cgggggccgc caagaccggc gcgctgggaa accgcgacat cgtggaggcc gttgccgccc
     1921 tcgcggcaga cttcgacttt ccccttgtcg tcgatccggt gatgatcagc aagcatggag
     1981 cgcggctgat gggagaggat gcccgcgatt cgatcgagag gctcttgctg cccgtgtctt
     2041 ttctgattac gcccaatctg ccggaagcag cggtactctc gggcatcgcc gtggagaacg
     2101 tcgacgacat gagggaagca gcgcggcggc tttgcggaaa aggcgcgcgc tccgtcctgg
     2161 tgaagggcgg ccacctggcg gaatccgcaa tggacatact tcttcatgag ggcgcatggt
     2221 acgaatttcc cgcgccacgg atcgaaacgc ccaacactca cggcacgggc tgtacctact
     2281 ccgccgccat taccgccgga ctcgcctccg gccggaaact gcccgatgcc gtagcgcggg
     2341 ccaagcgata catcgccgcg gcgatacaga acagtcccgg ccttggccat gggaccggcc
     2401 cggttcagca ccaggccagc ccggactgag cgcgggtgta gtagagtgac caaagtacga
     2461 aaaggaggca cttgtgaaat tcctggttgc gaccattgga atgctagccg ccgtgttgac
     2521 ggcatacgga aaaataccgg agcaggatag ccggaacacc gatattccgc acaccgacac
     2581 gcatttccgc gcgcgcagct acacgtcgct cgaggagtgg cagcagcggg cgccccagtt
     2641 gcggaaacag gtgctctccg ccgccggtct gcttcccttg ccggagcgca accccctgcg
     2701 atacgagatc ttcggacgca tcgagaacaa ggactactcg attgagaagg ttttcatcga
     2761 gacgatgccg ggctactatc tcgcgggcaa cctcttccgc ccgctcggcc gcacgggaaa
     2821 atttccgggc atcctcgcgc ctcacggcca tgcgacctac gggcggctgg agaatgggcc
     2881 tctcttttcg gtaccggcga ggggcattaa caaggcacgg cagggctacg tcgtcttcgc
     2941 ctacgatatg gtgggctaca acgacacgat tcaaactccg cacgtgtttg gcggcgagcg
     3001 cgaacagcta tggtcgttcg gtcccctggg tcttcaaacg tggaactcca tccgcgcgct
     3061 cgattttctt caggcgctgc cggacgtgaa cccgaacgag attggagtga cgggcgaatc
     3121 cggcggcggc acacagacat ttcttgtttg cgccgtggac gaccggatca aatactccgc
     3181 cccagtgaac atggtttccc tgatcatgca gggcggctca ccgtgcgaga acgcgcccaa
     3241 cctgcgcgtc gctaccaaca acgtggagat cgcctccctg atggccccga aaccgatgtt
     3301 gatggtatcg gccaccggcg actggacgaa gaacactccc accgaagagt atccggcggt
     3361 gaaggcgatc tacagcctgt acggcaagcc cgagaacgtg gacacgatac agattgacgc
     3421 cccgcataac tacaacaagc cgagccgcga ggcggtctac cggttcttcg ccaaacacat
     3481 cctgcacgac ccgaactggg cctcctactc ggaaagaaat atccgtaccg agaagttgca
     3541 ggacctgatg gtttttcacg ccaggccgct gcccgacaac gccctgaaat atgatcagat
     3601 cgccgcgcag tggatcgaac gcgcacagag gcagaacgct tcgatcaccg gccggtccgc
     3661 gatgcgcgag cgcctgatgt attcgctgat ggcggagcag ccccaagcgg tagagcacga
     3721 gatttccggc gaacgcatcc ttcttggccg tcagggaaag ggcgaccggg tgccgggcat
     3781 ctggattcca ggcggcaaac aggcgcttct ggttgtgcac ccggagggtg ccgccgcggc
     3841 ccgcaatagc aagcaggcga gcgacgccat ctcgaaaaag cagtcggtcc ttctgatcga
     3901 cgcgttccaa accggaagtg ccgccgctcc ccgggaccgt tcgaagcgtt actttctcac
     3961 tttcaataag agcgacgacg ccaaccgggt tcaggatatc ctgaccgcgc tggcattcct
     4021 gaaggaccag ggcgcttccg ggatccgtct ggcaggggtc gggaaagccg ccatctgggc
     4081 cacgtttgca gccgcgctct cggatgcagc cgtcaccctc gacgccgatc ttggttcgtt
     4141 ccagggcagc gacgaagact ttatcaaaga ttttttcgta cctggaattc agcgggcggg
     4201 tggattgaag gcggcattgg ctctcgcggg gtcaagccgg taggtgtgcg gacagggcgc
     4261 ggctgattat tcaaacgacg aacgcgtcgt gatgacgctg gacgcgggcg gtacgaattt
     4321 cgttttttcc gccatgcgat cgaatcgccc cgtcactgag ccgttcgcgc tgccgtcata
     4381 cggcggcgac ctcgaacagt cgctcaatac catcctcgag gggtttgacc gggtgcgcga
     4441 acaagcgccg tctcctcccg tcgccatcag tttcgcattc cccggtccgg cggactacga
     4501 aaacggcatc atcgcaggtc cacgaaatct gcccgcatac cggaatgtcg cgctcggccc
     4561 gatgctggaa gacacgttcg gtttgccggt tttcctcaat aacgacggcg acctgttcac
     4621 ctatggcgag tccatggccg gtttgttgcc ttttgtaaac cggatgctcg aacatgccgg
     4681 gagcccgaaa cgtttccaca acctgctggg cgttacgatc ggcaccggat tgggcggtgg
     4741 cattgtgcgc gacggcgagc tttttaaggg cgacaattcg attgccggtg agatgtggct
     4801 cttgcggagt aaggcggacc cctccaggaa tgccgaagag catgtgtcca tccgtgccgt
     4861 gcgccgcgcc tatgcgcaac ttgcacacat cggtttgtct gaagcgccgg agccgaaaga
     4921 gatttgcgat attgccgaag gccgcaggga aggagaccgc gccgccgcgg tggaggcgtt
     4981 ccggcgcctg ggagaggccg cgggcgatgc ggtcgcgcaa gcggtgacgc tggtggatgg
     5041 actggttgtg attggaggcg gcgtgtcggg cgcgcatccc ttgtttcttc cggctctggt
     5101 ggacgagatg aatgccacct ttctgacgcc ggacggcacc cggtttcgca ggctggtcca
     5161 gaggactttc aacctcgaag actccgctca gttgaagcag tttattcagg gggagcccca
     5221 agagctatgc gtccccggcg gaacgcgccg ggtgcgattc gacgcacaac agcggacggg
     5281 cgccggcatt tcccggctgg gaaccagcca ggccatcgcc atcggcgctt atgccttcgc
     5341 gctgaaacaa ctcgacagtg gcggctgagt cgcgatcgat ccaatccgcc agttccaacg
     5401 gctcgccaaa ctcccgaaat gcggcgtcga tcggctcctg gttgccccat acgacgtcct
     5461 gatactcgcc gcgctccgcc gccggctgaa tcccggcgca aaagagccta cggtcccgtt
     5521 ccacatagaa cccataggtg cggcatgcaa cgggccgttg atcgtaaata aggcagcttc
     5581 cgtcctgcaa atcgagcagc gggcagacga caggaggagc cgcgccacgc gtaaggtgga
     5641 tacgcgtccg aatcccgctc cgtctgtctt cgtcgagtac ggctagcccc tgccgcagca
     5701 gaagccactc ggcttcggtc aactttggcg ccgaagccag acgcctgcag caaagatcgc
     5761 atcccttgcg gcaaggccat ccgccggcac gggatgcaat gccgcaaaca cgcgcctcga
     5821 ttcgcccatg caagatccgt agctccgcag atgctcccaa atccggagtt tacaccggaa
     5881 agacaaagct atagaatcta ttcaacaacg taacggatta cctcttttga ggtaagggtg
     5941 agtaacaagt gaagagagaa ttggaaccgg tgcgcctgcg cgcacgggat accggcttgt
     6001 gcaaacgagc gctgataacc ggaggtatgg gctttatcgg cagcaacctt gctctgcggc
     6061 tggtggaact cggtgttgac gtgactgtcg tcgattcccg tgtggtcgat tgtgggagca
     6121 atccctggaa tctgagcggt gcggctcgcg agatacgggt gatcgagcgg gacatctcag
     6181 aagccgccgg cttgcagcgc gagatcagcg agagcgacgt cattttcaac ctcgccagcg
     6241 agatcagcca cattcacagc atgcagtatc ctcagcggga ctgtgcgctg aatgcagtag
     6301 ctcagttgcg cttcgtccag gcatgcgaac gcttcaatcc cggcgtccgc atcgtgtacg
     6361 ccagcacccg gcagatttac ggaatcccgc actatctccc ggtcgacgag ggtcatccgg
     6421 tacatccggt cgattacaac ggagtgcaca agtacgcggc gatgatgtac cacctgatag
     6481 cggcgcgccg cggccggttg gacgcagtcg ttctgtgcct cagcaacgtg tatggccccc
     6541 gtatggcgat caatattcca tgccagggcg ttctcggaaa ttttgtgcgc cgtctaatgc
     6601 agggcacgcg cctggaagtg ttcggcaacg gccgtcaatt gcgggatcct gtctatgtcg
     6661 atgatgtagt cgatgcattc ttgcgggcag cgggggtgcg gcgtccgcgt tcccgcacct
     6721 acaacttggg agggcccgat gcactcgccc tgcggaagat cgcacagatt gccagcagcg
     6781 cggccggtgt cgcagctccc gtcgttcatc ctttcccgaa agaacgtaaa tccttcgata
     6841 tcggcagcta ctataccgat tcgagcctca ttctgcggga gcttggctgg aaaccccgag
     6901 tgaagtttga ggaaggaata gcgcgcacac tcgagtacta ccgcaaagag ctgtcccact
     6961 atttggagcc ggacggcgaa cacaccggct gcaaactgac ggaacaaaaa tccgcccggg
     7021 ggatggtagc cgcggctcta tgatgaaccg ggcggagttt gcaaacatcg cccgtgccga
     7081 gcggcagtcc tggtggtatg ccggaatgcg ggagattctg ttgcgtacgc tcgatccctg
     7141 gctcgcgggc cggcacatcg aacgggtgct cgaggccggc tgcggcacgg gctacttcgc
     7201 gcatacgatg cagacggagc ggaaatggcc gattcacccg gtcgatcttg acgccgaggc
     7261 cgtgcggcgc gcccggacca tgggccttga acgcgtgacg caggccgatg tggcagcgct
     7321 gccttttcgc gattccgctt tcgaccttgt cctctctctc gacgttctcg tgcacttcgc
     7381 acgcggggag gagtacaggc cgctgtctga attgaagaga gtgcttgcgc caggagggct
     7441 attggccctg cgcgttgccg cgctcgatgt gctgcgaagc cggcattcgc aatttgtttg
     7501 ggagcgccag cgtttcaccc gctccaggct tctcaaagcg gtgcgacggt gcggcatacg
     7561 ggtgttgcgt tgcacctacg cgaacagttt gttgatgccc gtcgctctca ccaaattccg
     7621 cctgtgggag ccgatcctgc gcaagccgcc ttcgagcggc gtcgcacccg tttccgtttg
     7681 gctggaccgg cttctgcgtg ttccgctggc cctggaagca aagtggcttg gcagcgggat
     7741 cgacctgccc ctgggacaat cgttgctgct tatcggagag aagatgccgt gacagaagcc
     7801 ggcatcgggt ctttgaccgt gttctttccc gcctacaacg atgctccctc gcttccgcag
     7861 ctactgcaaa gaaccttcgc ggtgctccgg gagcacgcga ccgactacga agcgatcgtg
     7921 gtgaacgacg gcagccgcga caacaccgaa gcggtgcttc gggagttaca agcctgctat
     7981 gctccgcggc ttcgcgtgat tacacatccg cacaaccggg gctatggcgg agcgctgaga
     8041 agcggcttcg ccgccgccac caaggagttc gtcttctaca cagacggaga tggccagtac
     8101 gatccaacag agctgccgcg ccttctcgaa cggatgaaac caggcatcgg tctcgtgaac
     8161 ggctacaagc tcgagcggca cgatccgctg caccgcatct ggatcgggcg cgtgtacaac
     8221 cggctggcgc gtgctctgtt ccggattcgc atttccgaca tcgattgcga tttccggctg
     8281 atccgccggt ccctgctcga aggcatgaca ctcacctcca cgagtggaac catctgcgtc
     8341 gaactggtac ggaagatcga gctcagcggc tgcggtgtcg tcgaagtggg tgtccatcac
     8401 tacccgcggc tgcacgggcg ctcgcagttc ttccgcacac gctccctgct tacaaccttc
     8461 ggccaactgc tccggctatg gatacggctg gtagcggttc ccgccgtctt cgggaaggac
     8521 cggctccgct cgtgacagta cccatggtta atctgcggcc cctgctcgaa gagacacgtc
     8581 cccagtggac ggcaaacctc tctcgtcttt tcgagcgtat gcagttcatt ctgggcgaag
     8641 aggtcgcctg cttcgagcag gagctggccc gcagcttcgg ctccaggttt gcggtcgcga
     8701 ccggcacggg aacggcagcc atcgagttgt cgctgagggc ggcggaaatt gagggccgtg
     8761 aagttctcct gccggcgctc acctccctgt tcacggcaca ggcggtgctg gcggtccgtt
     8821 gcataccgcg gttcgccgac atccatcccg gcacgcttct gctggatgtt gccgacgtgg
     8881 agcgccgcat cacgccggct acggcagcga ttatcccggt gcatctttat ggacagccct
     8941 gcccgatgcg agcgctccgc cagattgctc gaagggccgg cctgacgctg gttcaggatg
     9001 gctgccaggc gcacggggcg cggcacgaag gggttgcact gacccgcttc tcgtcctgcg
     9061 tcgcttacag tttctacccg acgaagaacc tgggatgcct gggggacgga ggcgccgtgc
     9121 tgacggaccg ctcctctgta gcagaacgcc tgcgattact gcgcgacggg ggccgccgga
     9181 atggacaggt aagccgtctg cccgccatca attcgcgttt ggatgaaatg caggcctgct
     9241 atctgcgtgc gttcctgccg cggctggcgg aatggaatct ggagcgtgcg aggattgcat
     9301 cgctctacga cgaggcgctc tcggattgcc ccggcatcga gagcgtgcgg cgaagcacgg
     9361 actccgtgaa tcacctttac gtaatccgcg cccgccggcg cgaccggctt cgcgttcacc
     9421 tggccgcccg gggcatttct accgccgtgc attacccggt accgctgcat cttcagccgg
     9481 cgttccgcga atttggccct gcccggggcc aacttcccaa tgcggaaaaa gcgtgccggg
     9541 agattgtatc tctcccggtt tggccgaaaa tgccggagga tgccgtcctc cgcgtggcgg
     9601 ataacgttcg tgagttctac aaaaaatgat cgtggctgac agaattggcg aattgacggc
     9661 gcccttcagc gggcgccggt tgccggcggc aaagc
//
LOCUS       DASTMK010000238         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_394955, whole genome shotgun sequence.
ACCESSION   DASTMK010000238 DASTMK010000000
VERSION     DASTMK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394955"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1941)
                     /locus_tag="VFQ79_10480"
     CDS             complement(<1..1941)
                     /locus_tag="VFQ79_10480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU0140130.1"
                     /translation="MCFKGLILACAAGCILFAQSAPRSIRSFDVEAMDTSVDPCTDFF
                     QYSCGMWMKQNPIPPDQARWGRFDELQERNRHTLRDILEKAAVSNAGRTAVEQKIGDY
                     YASCIDEKTIEQQGVKPLAEELKRIDSLSAKSQLPETAARLHKAGVRALFRFTSAPDL
                     KNSAMMIADVDQSGLGLPDRDYYFKDDERSVELRKKYLEHVAKMFQLLGQSQETAAAS
                     ARTVMKMETALAKASLDRVARREPSNIYHKMSSKELQALAPSFGWNRYFEGTGTPAFD
                     SLNVSVPEFFKQVSSLAESTDLNDWKTYFRWHLVRAMAPVLSSAFVNENFDFYGRTLT
                     GAKALRPRWNRCVTYTDADLGEAVGQKYVERTFGAEGKARTLRMVRQLEKALEDDIRS
                     LDWMTPETKKRALEKLHAITNKIGYPDRWRDYSRLKIVRGDAFGNSLRANEFNFEWSI
                     RKIGKPVDPQEWHMSPPTVNAYYDPTMNNINFPAGILQPPFYDNKIDDAVNFGGIGAV
                     IGHELTHGFDDQGRKFDAKGNLNDWWTEQDAQAFEKRAACFVDQYAQYPATPEVKLNG
                     KLTLGENTADNGGLRIAYMALMDALEGRAGQKIDGFTPAQRFFISWGQVWCQNVTPEA
                     ARMRALVDPHSPGQWRVNGVVSN"
     gene            2198..>2654
                     /locus_tag="VFQ79_10485"
     CDS             2198..>2654
                     /locus_tag="VFQ79_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140131.1"
                     /translation="MNLSRIPGQRLKGDHIQTNTPRRYFNKIATMIAAVSGAPMLFAQ
                     RPATGTSRRSNHIHDGIYYFPGIGSNSGYPREDRILVTDPFEKHVTRTMDALKRSLER
                     VGCTMDSILHLEVYICLPHSDSVPMPAGNARFDAHTAQYQALNKIYGTYF"
BASE COUNT          510 a          813 c          736 g          595 t
ORIGIN      
        1 gttcgaaacc acgccgttga cgcgccactg gccgggtgaa tgagggtcta ccagcgcccg
       61 catccgggcc gcttccggag tcacgttctg gcaccacacc tggccccaac tgatgaagaa
      121 acgctgtgcg ggagtgaaac catcgatttt ttgcccggcc cgcccttcga gcgcatccat
      181 cagggccata taggcgatgc ggagtcctcc gttgtcggcg gtgttttctc ccagagtaag
      241 cttcccgttc agcttcactt ccggcgtcgc ggggtactgc gcatattgat ccacaaagca
      301 cgccgcgcgt ttttcgaatg cctgcgcgtc ctgctccgtc caccagtcgt tcaggttgcc
      361 cttggcatcg aacttacgcc cctggtcgtc gaatccatgc gtcaactcgt gcccgatcac
      421 cgcgccgatg cccccgaagt tcaccgcgtc gtcgattttg ttgtcataga acggcggctg
      481 aagaatgccc gccggaaaat tgatgttgtt catcgtgggg tcgtagtagg cgttcaccgt
      541 gggcgggctc atatgccatt cctgcgggtc gaccggcttt ccgatcttcc ggatgctcca
      601 ttcgaaattg aattcattcg cccgcaggga attgccgaag gcgtcaccgc ggacgatctt
      661 caggcgcgag tagtcgcgcc accggtccgg gtatccgatc ttgttcgtga tcgcgtgcag
      721 cttctcgagc gcgcgttttt tggtctccgg ggtcatccaa tcgagcgacc ggatatcgtc
      781 ttcgagcgct ttctccaact gccggaccat ccgcaacgtc cgtgccttgc cttcggcgcc
      841 aaatgtccgc tctacgtatt tctggccgac ggcttcgccc aggtccgcgt cggtataagt
      901 gacacagcgg ttccagcgcg gccggagcgc tttcgcgccg gtcagcgtcc ggccgtagaa
      961 atcgaagttc tcgttcacga aggccgagga gagaacaggc gccatcgctc ggaccaggtg
     1021 ccaccggaag taggtcttcc aatcgttcag gtctgtgctc tctgcaagag agctcacctg
     1081 tttgaagaac tccggcacgg agacgttcag gctgtcgaac gccggcgtgc ccgtgccctc
     1141 gaaataccgg ttccatccga acgaaggcgc cagggcctgc aactccttgg aagacatctt
     1201 gtggtaaatg ttggacggct cgcggcgtgc caccctgtcg agcgatgcct tggccagcgc
     1261 cgtctccatc ttcatgacgg tacgggcaga ggcggcagcc gtttcctgcg attgcccgag
     1321 caactggaac attttcgcga cgtgctccag gtacttcttg cgaagctcca cggagcgctc
     1381 atcgtctttg aaatagtagt cgcggtcggg caagccgagc ccgctctggt cgacgtcggc
     1441 gatcatcatc gcggagttct tcagatcggg cgccgacgta aaccgaaaaa gtgctcgtac
     1501 gcccgccttg tgcagccggg cggccgtctc cggcaattgc gatttcgccg aaagggaatc
     1561 gatgcgcttc agttcctctg cgagcggctt aacgccttgt tgctcgatgg tcttttcgtc
     1621 tatgcaggag gcgtagtaat ctccaatctt ttgttccacc gcggtgcgcc cggcattgga
     1681 gaccgcggcc ttctccagaa tgtcgcgcaa cgtgtggcgg ttccgctcct gcaattcgtc
     1741 gaaccgcccc catcgcgcct ggtccggcgg aatggggttc tgtttcatcc acatgccgca
     1801 ggagtactga aagaaatccg tgcagggatc cacactggtg tccatcgcct ccacatcgaa
     1861 gctccggatg gagcgcggcg ccgattgggc aaacaggatg cagcccgcgg cacaagcaag
     1921 aatcagccct ttaaagcaca ttcttccgat tatcctctac cgcggagcaa agtagctgag
     1981 ccgcgcgcgt cagcaagcgg ttggcggggc atcagataca ctccgaaacc tcattcatcg
     2041 ccgtgaattt tagccacgga tgaacacgga tgaacacaga taagtttcct gtttttcaat
     2101 ctgtgttcat ctgtgtccat ctgtggctct gaatctttca aaccttctta ccgtcgtggc
     2161 tccgccatca tcgcgagtgg agtgtgataa acctattttg aatctctccc gcattcccgg
     2221 gcagaggctc aaaggagatc atatccaaac caatactccc cgccggtatt tcaataagat
     2281 cgccacgatg atcgccgccg tttcgggcgc gccgatgctt ttcgcccagc ggccggcgac
     2341 aggtacatcg cggcgtagca accacatcca tgacgggatc tactatttcc cgggcattgg
     2401 ttccaatagc ggctacccta gagaagatcg cattctcgta acggacccat ttgagaagca
     2461 cgtcacccga acgatggatg cgcttaaaag atcgctcgag cgtgtgggtt gcaccatgga
     2521 tagcatcctc cacctcgagg tctacatctg tcttcctcac tccgatagcg ttccgatgcc
     2581 tgcggggaac gcgaggttcg acgcgcatac ggcacagtac caggcgttga ataaaatcta
     2641 cggaacttat ttca
//
LOCUS       DASTMK010000239         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_299744, whole genome shotgun sequence.
ACCESSION   DASTMK010000239 DASTMK010000000
VERSION     DASTMK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299744"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFQ79_10490"
     CDS             complement(<1..399)
                     /locus_tag="VFQ79_10490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU0140132.1"
                     /translation="MIDGGCPFCTPNSDRVFFESELVIGIWDAFPVSDGHALLVTRRH
                     VASWFDATPEERRALTDAIDATRAAILERHRPDGFNIGVNVGAAAGQTIFHLHVHVIP
                     RYAGDVGDPRGGVRHVIPRRANYLASSVGTK"
     gene            complement(396..905)
                     /locus_tag="VFQ79_10495"
     CDS             complement(396..905)
                     /locus_tag="VFQ79_10495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEU0140133.1"
                     /translation="MSSARERRALVQGRTYVRTLARLWQHPSLADVSLVLAPRLSRSA
                     GRCLPTRSSIELSAATLDSALAFRREVLCHEAAHAVVWARHGRKARPHGPEWQALMRQ
                     AGFEPRASLILCGRRAKRSTTTRFRHTCPVCHFSRTAKRRMTSWRCPECQAIGLDGEL
                     RIQRIASRR"
     gene            complement(889..1854)
                     /locus_tag="VFQ79_10500"
     CDS             complement(889..1854)
                     /locus_tag="VFQ79_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU0140134.1"
                     /translation="MEGWIAPTDYDWYSFLRDTSPYEEVNFWTPSDYFGFRGTPGAPF
                     LFKLKAAKSRKSTLPHNVIAGFGIVSRFAKLEDWLAWDCFGKGNGAADFSRMKARIDD
                     YRGRNELQGGSGVPRIGCIILSDAVFFPPELCIPQPADWPSQNLRPMRYDLSTGEGRR
                     IWEACQANLSAYSVRVAPELVARVMEATPRYGEPVLVQPRIGQGVFRIAVTDAYGRAC
                     AITGEHSLPALEAAHIKPYAAEGPHDVPNGLLLRSDLHRLFDKGYVTVTTDLQLEVSN
                     RLKEDYSNGRSYYPLHGQRITIPSDSRCAPSPEFLRWHNDHVFRA"
     gene            complement(1855..3411)
                     /locus_tag="VFQ79_10505"
     CDS             complement(1855..3411)
                     /locus_tag="VFQ79_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU0140135.1"
                     /translation="MIQHRDWAQEWLSENNRPLRISEARLARLALIASFSPANCLLRS
                     IESVYSADQVLDGFEEIVAVCLGAMRRYFNRTHVQQLIRSHRPRTRWHQFSGAAERGY
                     AKRVLVYAADAHLQAVLDEYVYLLCHAAQAETVSKVAERLEAVWGLVPGKPRANGAKG
                     RGNLVTIETEAEAHTSHFALSFGDDVWRGGDLEEEGEKVRKSAVREAFNSPFWPFVLA
                     TTSVGQEGLDFHLYCRDVLHWNLPSNPVDLEQREGRVNRRDCLAVRESIALDWRLNDP
                     ATWGNADRARNPWPFVFDSILASDDHQKYKHGLFPRWIYECRDPSRTVRIQRHVPVFS
                     TSRDAAKYERLKTGLALYRLVFGQANQEDLLDDLQSHVESLSSFEQEQLLKRLASYML
                     NLSPISHEQALRHAENEADDLLREGSGAGLQKLIVDVERMRAERQSELASIDSEVSGL
                     LQLVRRATAMPDSGEGAAVRSAIAALAYLRNPYDRVFDLHVEGGFVDDVMVIKDAWAT
                     VLGTASSERS"
BASE COUNT          658 a         1130 c         1071 g          651 t
ORIGIN      
        1 cttcgtgccc acgctactcg cgaggtagtt ggcgcgtcgc gggatcacgt ggcggacgcc
       61 gccgcgcggg tcgcccacgt cgccggcata ccgcgggatg acgtgcacgt ggagatggaa
      121 gatcgtctga cccgccgccg cgccgacgtt gacgccgata ttgaaaccgt ccggacgatg
      181 gcgctcgagg atagccgccc tggtggcatc gatcgcatca gtcagggccc gccgctcctc
      241 aggcgtggca tcgaaccagg aagcgacatg ccggcgcgtc accagcagcg catgaccgtc
      301 ggacacgggg aacgcgtccc agatgccgat caccagttcc gactcgaaga agactcgatc
      361 cgaattcgga gtacagaagg ggcaaccgcc gtcgatcatc gtctggacgc tatcctctgg
      421 atgcgaagct caccgtccag cccgatcgcc tggcattcag ggcaccgcca actggtcatg
      481 cgccgtttgg ctgttcttga gaagtggcag accgggcagg tgtgccggaa gcgcgttgtc
      541 gtgctgcgct tagcgcggcg gccgcatagg atcaggctgg cgcggggttc gaagcccgcc
      601 tggcgcatga gcgcctgcca ttcgggtcca tgcggcctcg ccttgcgtcc gtgtctcgcc
      661 cagacgacgg cgtgcgcggc ttcgtggcag agaacttcgc ggcggaacgc caaggcactg
      721 tccaatgtgg cagcgctgag ctcgatggac gatcgggtcg gcaagcatcg acccgccgag
      781 cgggagagac gtggggcgag tacgagactc acgtcagcga gggatggatg ctgccagagc
      841 cgcgccagtg tccgaacgta cgttcgcccc tgcaccagcg cccgtcgctc acgcgcggaa
      901 gacatggtcg ttgtgccagc gcaggaactc cggcgagggt gcgcagcggc tgtcggaagg
      961 aatcgtgatt cgctggccgt ggagcgggta gtacgaccgg ccgttgctgt agtcctcctt
     1021 caaccgatta ctgacctcga gctgcaggtc ggtagtgacc gtgacataac ccttgtcgaa
     1081 gaggcggtgc agatctgaac ggagcaggag gccattgggg acgtcatgcg ggccttcagc
     1141 cgcatacggc ttgatgtgcg ccgcttcgag cgccggcagc gaatgctcgc cagtgatcgc
     1201 gcaggcccgg ccgtatgcgt cggttacggc gatcctgaac actccctgcc ctatccgcgg
     1261 ctgcacgaga acgggctccc cgtaacgagg tgtcgcttcc atgacccggg ccaccagttc
     1321 cggcgccacg cgcactgagt aggccgacag gttcgcctgg caggcctccc agatccgtct
     1381 cccctcgccc gtggagaggt cgtatcgcat gggacggagg ttctgggacg gccaatccgc
     1441 cggctgcggg atgcacagct ccggcgggaa gaagaccgca tcactgagaa tgatgcagcc
     1501 gattcgagga acacccgaac caccctggag ttcgtttcgg ccgcggtagt cgtcgatgcg
     1561 ggccttcatg cggctgaagt ccgccgcacc gttacctttg ccgaagcagt cccaggcgag
     1621 ccagtcctca agctttgcga atcgggacac gatcccgaag ccggcgatga cattgtgcgg
     1681 aagcgtagac ttcctgctct tggcggcctt cagtttgaaa agaaatggag cgccgggagt
     1741 gccgcggaat ccgaaatagt cggatggtgt ccagaagttg acctcctcgt acggactggt
     1801 atcacggagg aatgagtacc agtcgtagtc cgttggtgcg atccagccct ccattcagga
     1861 tcgctcgctg ctcgcagtgc cgaggacggt cgcccatgcg tccttgatga ccatgacgtc
     1921 atcgacaaat ccaccttcca cgtgcagatc gaacacgcgg tcgtacggat ttcgcaaata
     1981 cgccagagcg gcgatcgctg accgcaccgc agccccctcg ccgctgtctg gcatcgccgt
     2041 cgcgcgacgt accaactgca ggagaccgct cacctccgag tcgatcgatg ccagttcgct
     2101 ttgccgctca gctctcatcc gctcgacgtc aacgatcaac ttttggagcc ccgcaccaga
     2161 tccttcgcgg agcaaatcat cggcttcgtt ttcagcgtgg cgcagtgcct gctcatggct
     2221 gatgggggac agattcagca tgtagctcgc caagcgtttg agcagctgct cctgctcgaa
     2281 agaagacagg ctctcaacgt gagactgaag atcgtccagc agatcctcct ggttggcttg
     2341 accaaacacc aggcgataaa gtgcgagccc cgttttcaaa cgctcgtact ttgccgcgtc
     2401 tcgacttgtg ctgaagaccg gcacatggcg ttgaatccga accgttctgg aggggtctct
     2461 acactcgtag atccaccgtg ggaacagacc atgcttgtac ttctgatggt cgtcgctggc
     2521 gagaatgctg tcgaagacga aaggccacgg gtttctcgcc cggtccgcat ttccccaggt
     2581 ggcggggtca ttgagccgcc aatccagagc aatagactcc cgcacagcca ggcaatcgcg
     2641 tcgattgacc cggccctcgc gctgctcaag gtccacagga ttcgatggca ggttccagtg
     2701 gagcacgtcg cgacagtaca agtgaaagtc gagtccctcc tggcctaccg aggtcgtcgc
     2761 gagcacaaac ggccaaaacg gcgaattgaa ggcctcccgg actgcgctct ttcgaacttt
     2821 ctcgccttcc tcctccaaat cgccgcctcg ccagacatca tctccgaacg acagcgcgaa
     2881 gtgcgacgta tgcgcctccg cctccgtttc gatcgtgacc aggttgccgc gcccttttgc
     2941 gccattcgcc cgcggctttc ctggcaccag gccccacaca gcctctagcc gctctgccac
     3001 cttacttacc gtctcggctt gggccgcgtg acacagcagg tagacgtact catccagaac
     3061 tgcctgcagg tgtgcgtcgg ccgcatagac cagcacacgt ttggcatatc cacgttcggc
     3121 cgcgccgcta aactgatgcc agcgcgtacg cggccgatgg gagcgaatca attgctggac
     3181 gtgtgtgcgg ttgaagtacc gccgcatcgc cccaaggcag acggccacga tttcctcgaa
     3241 tccgtccagc acctggtcgg ccgaataaac ggattcaatg ctgcgcaaga ggcaattcgc
     3301 tggcgagaat gatgcgatta gcgccagccg cgccagtcgg gcttcggaga ttcgtagggg
     3361 ccggttgttc tccgacaacc attcttgcgc ccagtcgcga tgctgaatca ccgattccga
     3421 ctctccgtcc cttcccgcgt ctgacgccca cgcatccaag gccgcctcga ttaggcgctc
     3481 ccggccgctt agtcgttccg acccgagaac
//
LOCUS       DASTMK010000240         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_554295, whole genome shotgun sequence.
ACCESSION   DASTMK010000240 DASTMK010000000
VERSION     DASTMK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554295"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1195
                     /locus_tag="VFQ79_10510"
     CDS             71..1195
                     /locus_tag="VFQ79_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0140136.1"
                     /translation="MPRSTVAALYTSPERVLDDIGRLAELAGMRDALDARSTTILKDN
                     ISWHFPFPGANTTPWQLEGTILALRERGFRDLSCVQNKTVVTNAFKGEDLNHYVPVFR
                     RYEIPVLYNFHDADMKWIEYKPKGKMLVLDKIFREGIYIPDYFFGKNIVHLPTVKCHI
                     YTTTTGAMKNAFGGLLNTHRHYTHSWIHETLVDLLTIQKEIHSGLFAIMDGTTAGDGP
                     GPRTMRPKIKNLMLASADQVAIDAVAAKIMGFDPMSLPYIRLAHERGLGTGKPEEIHI
                     AGDDIRNESWGFSVGDNMASRIGDVCWFGPLKRIQKLLFHSPLVHMFVFGSEAYHDWF
                     RWPVKDRRVYEQWLRTTEWGRLFGEYARYGTRTSAASAAD"
     gene            1364..2527
                     /locus_tag="VFQ79_10515"
     CDS             1364..2527
                     /locus_tag="VFQ79_10515"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0140137.1"
                     /translation="MRVAIDATCWHNNRGYGRHARALVSTLIRRYPENEYRLFLDSAG
                     NTPDLPGHAKLELVAADTPASAAASSNSHRSLSDMWRMSRALSKAGADVLLYPTIYTY
                     VPALTRARKLVMIHDVIAETYPNLTLPRRTARLFWKAKVALGRMQADALVTVSEFSRE
                     KIIEYFRVAPERVFVVSEAADPGFRILEQPAIPASLRALGIDPSRRIVVYVGGFSPHK
                     NLEELMDAFSHIASLGQFDDCLLVLVGETRKEVFHSCLPALRAKVDQAGLRERVVFPG
                     YVSDEDLTELLNLSAVLVLPSFMEGFGLPAVEAAACGCPVIATKASPLPQLLEGGGIY
                     IEPGSGQLEQALEVVLASEEMRERMRRAGAAAAQQLTWTAAAEQMMQVMQRFE"
     gene            2536..3816
                     /locus_tag="VFQ79_10520"
     CDS             2536..3816
                     /locus_tag="VFQ79_10520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0140138.1"
                     /translation="MNFLHLTTFYPPYSFGGDAMYIYRLSHALAEAGHHVDVIHCVDS
                     YHLLHPAEPPIQFAEHPNVTRHELRSGYGWLSPFLTQQTGRPYLKMDTIRKVLSSRRH
                     DVIHFHNMSLLGPQALAIEPPNPEAVKIYTTHEHWLVCPMHVLWKFNSRACEKPECLK
                     CTLLGKRPPQWWRYTGLLERSARRVDQFVSPSRFTAKMHASRGFPQPVEHLPYFIDRA
                     DEDWRTPGPRPQERPYFLFVGRLEIIKGLQTLIPLWKNMEFDLLVAGTGNYEVELRAM
                     AASNPRIRFLGPMSQRQLGALYHHALACILPSITYETFGMISIEAFARKTPVIVRDLG
                     ALPEAVEDSGGGFVYRSEEELLEAIRQIGASPRLRSELGEKGYRGFLEWWCREAHMRF
                     YFDYLRRAAARKSGRVPWQTAPEPVCSQPSSPKA"
BASE COUNT          779 a         1218 c         1215 g          805 t
ORIGIN      
        1 ccggagttac actgccgcca ggggacgcgt gcaggttgcc atcatggggg aagaataagg
       61 ggggccgttg atgccgcgaa gtactgttgc cgctttatat acatcgccgg agagggtgct
      121 cgacgatatt ggacgcctgg cggagctggc aggaatgcgc gatgcgctgg atgcgcgctc
      181 gaccaccatc ctgaaagaca atatctcctg gcactttccg tttcccggtg caaataccac
      241 gccctggcag cttgaaggca cgatcctggc gctgcgcgag cgcggattca gagacctctc
      301 ctgcgtgcag aacaaaacgg tggtcaccaa cgcgttcaaa ggcgaggatc tgaaccacta
      361 cgtgcctgtt ttccggcgtt acgaaatccc ggtgttatac aacttccacg acgcagacat
      421 gaagtggatc gagtacaagc ccaaggggaa gatgctggtg ctcgacaaaa tcttccgcga
      481 agggatctac attccggatt atttcttcgg gaagaatatc gttcatctgc cgacggtgaa
      541 gtgccacatc tacaccacta caacgggcgc gatgaagaac gcgtttggcg ggctcctgaa
      601 cacccatcgc cattacacac actcctggat tcacgaaacg ctggtggatc tgttgacgat
      661 tcaaaaagag atccactctg gactttttgc cattatggac ggcaccacgg ccggcgacgg
      721 tccggggccg cgcaccatgc gtccgaaaat caaaaacctg atgctggcca gcgcggacca
      781 ggtggccatt gatgccgtgg cggcgaagat catgggattc gacccgatga gcctgcctta
      841 tatccgcctg gcgcacgagc gcgggttggg caccggcaag cccgaggaga ttcacatcgc
      901 cggcgatgac attcggaatg agagctgggg cttcagcgtc ggcgacaaca tggcgagccg
      961 catcggcgat gtctgctggt tcgggcccct gaaacgcatt cagaagctac tgtttcattc
     1021 gccactggta cacatgttcg tttttggatc agaggcctac catgactggt tccgctggcc
     1081 ggtcaaagac aggcgggtct acgagcagtg gctccggaca accgaatggg gccgcctgtt
     1141 cggcgaatac gcgcgatacg gtacgcgcac atccgccgct tcggcggcgg actgagtacg
     1201 tggtttcgca aatacggttg cgtgttctcg ctttacgtgg acaccgctcc ctgacggtcg
     1261 cggctcagta ttggcctcat tccgagccgc gaccgtcagg gagcggtcgt tcggcatacg
     1321 tcaccgtaat tccgaacgtg agtactgagc ctggtgtgaa accatgcgtg tcgccataga
     1381 cgccacctgc tggcacaata accggggcta tggacggcac gcccgcgcgc tggtttcgac
     1441 gcttatccgc cggtatccgg agaatgaata ccggctgttt ctggactcgg ccggcaacac
     1501 gcccgatctc cccggacacg cgaaactgga actggtcgct gcggacacgc cggcctcggc
     1561 ggcggcctca tccaacagcc accggtcgct gtccgatatg tggaggatga gtcgggcttt
     1621 gtcgaaagcc ggcgccgacg tgctcctcta tccgaccatc tatacatatg tcccggcact
     1681 gacgcgcgcc cggaaactcg tcatgattca tgacgtgatc gcggagactt atccgaatct
     1741 gaccctgccc cggcggacgg cacgcctgtt ctggaaggcc aaagtcgcct tggggcgtat
     1801 gcaggcggac gcactggtga ccgtctccga gttctcgcgc gagaaaatca tcgagtattt
     1861 ccgtgttgct ccggaacgcg tctttgtcgt cagtgaagcc gcagatccgg gtttccgtat
     1921 cctggagcag ccggccatcc ccgcatccct gcgggcgctc ggcatcgacc ccagccgccg
     1981 catcgttgtt tacgtcggcg gcttcagccc gcacaagaat ctggaggagc tgatggacgc
     2041 gttctcgcac atcgcttctc tcgggcagtt cgacgattgc ctgctggtgc tggtgggaga
     2101 gacgaggaaa gaagtgtttc acagttgcct tccggccctt cgcgcgaaag tggaccaagc
     2161 cggcctgcgc gagcgcgtcg tgtttccggg atacgtatcc gacgaggatc tgacagagtt
     2221 gttgaacctt tccgctgtgc tggtgcttcc ctctttcatg gaaggcttcg gactgcccgc
     2281 ggtggaggcg gcggcgtgcg ggtgcccggt gatcgccacc aaggccagtc cactgccgca
     2341 actgctggag gggggcggaa tctacatcga gccggggagc ggccaactgg agcaggcgct
     2401 ggaagtggta ttggcctcgg aagagatgcg ggaacgcatg cggcgggccg gcgccgcggc
     2461 ggcgcaacag ctcacctgga cagccgcggc agagcagatg atgcaggtga tgcaaaggtt
     2521 cgagtagcca ctgccatgaa ctttctccac ctgaccacgt tttatccgcc ttacagcttc
     2581 ggcggcgatg ccatgtacat ctaccgcctc tcgcatgccc tggcggaggc gggtcatcac
     2641 gtggatgtca ttcattgcgt tgattcctat caccttctgc atccggcgga gccgccgatc
     2701 caatttgccg aacacccgaa cgtgacgcgt cacgagctgc gcagcggcta tggctggctg
     2761 tctccgttcc tgacccagca gaccggccgt ccgtacctca aaatggacac gattcgtaag
     2821 gtgctttcct cgcgccgcca cgacgtgatc cacttccaca acatgtcgct gctggggccg
     2881 caggcgctcg ccatcgagcc gcccaatccc gaagcggtga agatctacac gacgcacgaa
     2941 cactggctgg tctgtcccat gcacgtgctg tggaagttca acagcagggc gtgcgaaaaa
     3001 cccgagtgcc tgaagtgcac gctcctcgga aaacggccgc cgcagtggtg gcgctatacc
     3061 ggtttgctcg aacgctcggc gcggcgcgtg gatcagttcg tctcacccag ccgcttcacg
     3121 gcgaagatgc atgccagccg gggctttccg caaccggtgg aacatctgcc gtatttcatc
     3181 gaccgcgccg acgaggactg gcgtaccccc ggcccacgcc cgcaggaacg gccctacttc
     3241 ctgttcgtcg gccggttgga aattatcaaa gggctccaga cgttgatccc gctttggaag
     3301 aacatggagt tcgatcttct ggtggccggg accggcaatt atgaggtgga actgcgcgcc
     3361 atggcggcat cgaatccgcg gattcgattt ctgggcccca tgtcacaacg gcagttgggc
     3421 gcgctctacc atcacgcgct cgcctgcatc cttccttcga tcacttacga gaccttcggg
     3481 atgatcagca tcgaagcctt cgcacgaaag acgcccgtca tcgtgcgcga cctcggcgcg
     3541 ctgcccgaag ccgtagagga ttccggcggc ggattcgtct atcgaagcga ggaagaactg
     3601 ctcgaagcca tccggcagat cggcgcttct cccaggctgc ggtccgagct tggggagaaa
     3661 ggctaccgtg gttttctcga gtggtggtgc cgggaggcgc acatgcgctt ctacttcgat
     3721 tacctccgcc gggcggcggc gagaaaatcc ggacgcgtcc cctggcagac cgcacccgaa
     3781 ccggtctgct cccagccgtc cagtccaaaa gcttaggaat ctggtctctt tttttgaaaa
     3841 gaggccggct tcccagcgcc aaaactctcc attttacggg ctccagttgg gccctgtacg
     3901 tgccttcctg cctgtaatac ccatgatttg caatctggag gcatggttga cgaggttcgc
     3961 atggcaacat ttctagtaac cggaggggcc gggtttatcg gctcccatct cgtggaa
//
LOCUS       DASTMK010000241         7822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_427366, whole genome shotgun sequence.
ACCESSION   DASTMK010000241 DASTMK010000000
VERSION     DASTMK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427366"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFQ79_10525"
     CDS             <1..358
                     /locus_tag="VFQ79_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEU0140139.1"
                     /translation="TALGKVLLAYGSEDQLKAVVARGLSRLTSKTITQPERLVSELKR
                     VRERGYAVDDEEFYRGIRCVAAPVFDDDATITAAMSVSGLITHVTYDRLPQFAEAVMH
                     AARELSQQLGFQPTQT"
     gene            complement(349..2001)
                     /locus_tag="VFQ79_10530"
     CDS             complement(349..2001)
                     /locus_tag="VFQ79_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140140.1"
                     /translation="MKTVPYRLRKHVASTVNIVDILTLLGYTVMLIGIASWHSRRITG
                     RDDYFLAGRSMSRWPIAMSMYVALFSTNTFIGVIGWVNRPDGTLWIGLQNIGIILAVP
                     LVVALYPDIFFRLRISTAYEYLEKRFSYPVRAVAAVLFMGSRVMWMATMLYGGGLVIS
                     QLLGWTAANGVPSGHVYAVILLGALGTFFALGGGIRAVIWTDVAQFFVLFGAVAVMVV
                     LAVARSGGPAHILAVAGEAGKFSPPQFFSFTDDLSIVSGLCLGLIGLLSSAGTDQVLL
                     QTYLSARNVGEAKKSLWFNGLFLKPLSLIFPVLGVIIFAYFHEHPQEAALMRIADDAL
                     PVFVLNVLPAGVRGLAIAALMSALLTSVGSGLAALSSSLQIDFVQRLMKRRLSERAAV
                     RLGRTLTFLWGVVIVSAAMLVLRLGAKNNIIQILNIVMYPFAGVLLGIFLCGLLTRRV
                     NGGGTLIGALGGFALTVGAPRLHLGATLVDSTGVLFPPDLMSGLSWLSRVSSFYYGVL
                     GTMATLAVAYIASLFFAPPRNENLEGLTHARGSKRERVASQV"
     gene            2173..5637
                     /locus_tag="VFQ79_10535"
     CDS             2173..5637
                     /locus_tag="VFQ79_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0140141.1"
                     /translation="MKHKGSLIAMLLLSFSSANAQSTFGSILGRVLDSSGSAVAGASV
                     RVINRDTGVVVTATANQDGSYEAPYLVPGSYSVSVEHTGFKKFIQQDVTLRVSDRIAV
                     NITLEVGEVTETVNVTDAPPLVETTNASIGSVVDSKRIQDLPLSDGNPFILAHLAPGI
                     LNLGNPANQRTFDNDSTSDIAANGTPTRRNEFQLDGVPDTAGRIVAFIPSTDMVREFR
                     VQTASYDAGYGNTPGAIVNVSLKSGTNNVHGSLWENLRNSATDASSFFTNRAGQEKPA
                     FRLNHFGASVGGPVFLPKLYDGRNRTFFFFGFEGLKDAYPQPLTGTVPTLRMREGDFS
                     ELLALGSRYQIYDPSTIVPAAGGRFSRQPLPGNLIPRDRLNPTALAYMQYWPEPNQPG
                     TVDFQQNYYNGQRLKTDDFYSYLGRVDHNIGESHRFFARLNYNQLHEVANNNFNNIAT
                     GTNRFRKNHGAAIDDVWIASPSTVVGFRYGYTRWSERRPALSDGFNLAGLGFSPEWAN
                     YRSADIRTMPPIGVSGMSGLGGTWGYIEAYDTHTFGGNVTKTIGSHSIRFGGDMRVYR
                     ETRRDEGNAVGSFNFGTTWTRGPLDNSAAAPTGQGLASFLMGLPDGGNADINASYAEQ
                     SVMSSLFIQEDWKATRRLTLNFGLRYELETPITERFNRTLRGFDYDAPSPIEAAAREV
                     YARSPIPEVPVSEFRVRGGLLFAGQNGVPRGLWEMDKNNLQPRIGFAYQLRDATVLRG
                     GYGIFHDFLGLRVGTVDVIQSGYSRRTDFVASLDNGQNFIANLTNPFPNGLLEPLGSS
                     LGLSANLGQAVTFLRTNPKTGYMQRWSLNVQHEIPGNVLIDIGYLGNRGTGLPMIREL
                     NYVPNQYLSTLPVRDDPANNFLTAQVPNPFAGLLPGTSLNGNTVSRSVLLTGYPHFSS
                     VRRNIESIGYSWYHSLQTRVEKRLGSGLVMGGAWTWSKALAAMSFRNAGDPFLEEVLS
                     SQDRTHRLAVNWVYDLPIGRGRRIGSNFNAVADAILGGWQVAGIYQAQSGAPLGIGDP
                     VLTGPFSVTDLILPEDQRIPERYINTDVDLNKLPARAFVSHLNTMSSRFGALRSDGMN
                     QWDISVAKTWRLTESVQLRFQTQFINAFNHVTFSAPNLTSTNTAFGTVTSEASMPRTI
                     RWGLRIEY"
     gene            5645..7678
                     /locus_tag="VFQ79_10540"
     CDS             5645..7678
                     /locus_tag="VFQ79_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU0140142.1"
                     /translation="MNHRLFAIAVLAPALLAQQAAVVPPQYPVASNPDFLLNKPSEDP
                     LTKRYAWIPKPLYYGGKDTVGPLDADWVWPDIALVDWNRDGLLDLVCALTAGTYSHRP
                     KDQQFRALVYRNTGQREGRTPVFDEPYQVDLSFPIGSMVFDDIDNDGTLDLIMWSRNL
                     FRWYRDTSKSGERRFEFAGNLQDYGLHTDLKLLESVSMSPSLQLVDWDGDGRKDLIVG
                     GRGGNVNYYPKLEVGFGKGFAPDGSWLGGDRNGSVVFHKNLGVRDGKLTFSTGKRLTA
                     GEDERAISYFDTATAAVTDYNDDGLPDIVVASFDNLFWYENVGAPGRPMLISGKRIPV
                     AAGERLPWERVLPIVANWNSSDRNHLILQGSSYAWYLPNSGNKGMPRYEAITQLLQRN
                     PPVGAGDFAIVAAGDLNGDGKPDLVIGNEDGYLLYVENTSKGNEPETFKPAVQLKSAG
                     EVFRIESGRGLQGPAEARWGYTIPVLTDWDNDGDLDLIVGSLHSYYLLLENTGTRSTP
                     VFGKPEPLTSGGETLLLPWRTRPVVHDFDGDGQKDLLALDPEGMLTLYRRSGAREVQA
                     GKRILDQNNSPIKLDGKGRETGRAMLAVMDWDEDGKLDLIAGNAIENFDGLRWYRNIG
                     TNTKWVLARQPNLALNLPWNHYHQIEPVDWNRDGKLDLIAGSEGGWVYYYPQR"
BASE COUNT         1700 a         2299 c         2281 g         1542 t
ORIGIN      
        1 tacggcgctg ggcaaagtcc ttttggctta tgggagcgaa gatcaactga aggcggtcgt
       61 ggcccgcggc ctctcgcgcc tgacgagcaa aacaatcact cagccggagc ggctcgtaag
      121 cgagctcaaa cgtgttcgcg agcgcggtta tgcggtcgat gacgaggagt tttaccgggg
      181 aattcgctgc gtcgccgcgc ccgtattcga cgacgatgca acgattaccg cggccatgag
      241 cgtgtccggg ctgatcacgc acgtaacata cgatcgtctt ccgcagttcg ctgaagccgt
      301 gatgcatgcg gcgcgcgaac tctcgcagca gctcggattc cagccaactc agacttgaga
      361 tgcgacccgc tcccgtttgc tcccgcgggc atgcgtcagc ccttcgaggt tctcgttgcg
      421 cggtggagcg aaaaacaggc tggcaatata tgctaccgcc agggttgcca ttgttccgag
      481 aactccgtag tagaagctcg aaaccctgct gagccaactt agacctgaca tcaagtccgg
      541 tgggaaaagg acgccggtag agtctaccag cgttgctccc aagtgaaggc gaggcgcgcc
      601 cactgtgagt gcaaagcctc cgagtgcacc aatcaatgtt ccgccgccgt tcacgcggcg
      661 tgtaagcagc ccgcagagaa agatacccag tagcacgccc gcgaacgggt acatcacgat
      721 gttcaggatt tggatgatgt tgttcttcgc cccgagacgg aggaccagca tggccgcgct
      781 gacgatcact acaccccaca ggaaagtcaa agtacggccg agccgcaccg cggctctctc
      841 cgaaaggcgc cgcttcatca gcctctgtac gaagtcaatc tgaaggcttg aggacaaggc
      901 ggcgagtccg cttccgacgc tggttaacag cgctgacatg agggcggcga tggcgagccc
      961 acgcactccg gcgggcagca cgttcaaaac aaaaaccggc agcgcgtcat cggcaatccg
     1021 catgagcgcc gcctcttgtg gatgctcgtg aaaataggcg aagatgataa cgccaagaac
     1081 ggggaatatg aggctgagag gctttaggaa caggccgttg aaccatagcg atttcttagc
     1141 ttcccctacg ttcctggcgc tcagatacgt ctgtagcagc acctgatccg tgcccgcgct
     1201 tgacagtaga ccgatcaggc cgagacagag gccgctcacg atactcaggt cgtccgtgaa
     1261 gctgaagaat tgcggcggag agaacttacc ggcttcgccg gcgacggcga ggatgtgagc
     1321 cggcccaccg gagcgcgcca cagcgagcac caccatcacc gcaaccgcac cgaagagcac
     1381 gaagaattgc gctacgtcgg tccagatgac cgctcgtatg ccgccgccga gtgcgaaaaa
     1441 cgtgcccagc gctcccagca ggatcactgc atatacatgg ccggaaggga cgccgtttgc
     1501 cgctgtccag ccgaggagtt gggagatcac cagcccaccg ccatacagca tcgtcgccat
     1561 ccacatgacg cgcgatccca tgaacaacac cgccgctacg gcacgaacag gatagctgaa
     1621 acgcttttcc aggtattcgt aggccgtact gatgcgcagc ctgaaaaaga tatcgggata
     1681 cagtgcgacg acgagcggca cagcgagaat aatgccgata ttttgcaggc cgatccacag
     1741 cgtgccgtca ggccggttta cccaaccgat gacgccgata aaggtattcg tgctgaatag
     1801 cgcgacgtac atgctcatgg cgatcggcca ccggctcatg ctgcggcccg ccaggaagta
     1861 atcgtcacgg ccggtaatgc gccgtgagtg ccaggatgct atgccgatga gcatcaccgt
     1921 atatccgagc aatgtaagga tgtcgacgat attgactgtg gatgccacgt gtttccgcaa
     1981 ccggtatggt acagtcttca caccgcccat tcctcgcccg gaagacgatt gttctatcgt
     2041 acggaacgtt aatggatctt gttctaccag ccagaacaac cgcgcgccgt ctattgaact
     2101 ccgtgcgctc cgcgctgaac atgaagagca agagtacagt cgcaggtgag agtaagggac
     2161 ggtggtgaag ccatgaaaca caaagggtct ttgattgcta tgctgttgct gtctttcagc
     2221 agcgcgaatg cacagtccac attcggcagt attttgggaa gggtcctgga ttcgagcggc
     2281 tcggcggtag ccggcgcttc cgttcgagtc atcaatcgcg acaccggcgt agtcgtgacc
     2341 gctaccgcaa accaggacgg cagctacgag gcgccgtacc tggtgcccgg atcgtacagc
     2401 gtttcagtgg agcacacggg cttcaagaag ttcatccaac aagatgtgac gcttcgcgtc
     2461 tccgatcgca tcgccgtcaa tatcacgcta gaagttggag aggtgacgga gacggtcaac
     2521 gtcaccgatg cgccgcccct agtggagacc acgaatgcgt cgattggctc tgttgtcgat
     2581 tcaaagcgca ttcaggattt gccgctcagc gatggcaacc ctttcatcct ggcgcatctc
     2641 gcgcccggga ttctcaatct gggaaacccg gcaaatcagc gtaccttcga caacgactcc
     2701 acatctgaca tcgcagccaa tggtacgccg acgcgcagaa atgaattcca gttggacggt
     2761 gttcccgata ccgccggccg catcgtcgca tttatcccat cgacggacat ggtccgggag
     2821 tttcgtgttc agacagccag ctacgacgca ggatatggca acaccccggg tgcgatcgta
     2881 aacgtgagtc tcaagtcggg aacgaacaac gtgcacggct cgttgtggga gaacctgcgg
     2941 aattcggcga ccgacgcaag ctcgttcttc acaaaccggg ccggccagga aaagccggca
     3001 tttcgactga atcacttcgg tgcttctgtc ggcggcccgg tattcctacc caagctctat
     3061 gacggtcgca atcggacatt cttctttttc ggtttcgaag gactcaagga tgcataccca
     3121 cagccgttaa caggcaccgt tcccacgctc aggatgcggg agggggattt ttccgaactg
     3181 ctggcgctcg gcagccggta tcagatctac gatccatcca cgattgttcc cgccgcaggc
     3241 ggccgctttt cgaggcagcc gttgccggga aacctcatcc cgcgcgatcg tctgaacccc
     3301 actgcactgg cgtatatgca gtactggccc gaacccaatc agcctggaac cgtggatttc
     3361 cagcagaatt actacaacgg gcagaggttg aaaacggatg acttctactc gtatctcggc
     3421 cgcgtggatc acaacatcgg ggagagccat cgattcttcg cccggctgaa ttacaaccag
     3481 ttgcacgaag tcgcgaacaa caacttcaac aacatcgcaa ccggcacgaa ccgctttcgc
     3541 aaaaatcacg gcgccgcgat cgatgatgta tggattgcca gtccctcgac cgtcgtcggc
     3601 ttccgatacg gctacacccg gtggtccgag cgtcgtcccg cgctgagcga cgggtttaac
     3661 ctcgcgggcc tcggtttctc gccggaatgg gccaactacc ggtccgccga tattcgcacg
     3721 atgccgccga tcggtgtgtc agggatgtcc ggcctgggag gcacctgggg gtacatcgag
     3781 gcctacgata cacatacgtt tggcggcaac gttacaaaga ccatcggtag ccacagcatc
     3841 cggttcggcg gcgacatgcg tgtctatcgc gagacgcgcc gagacgaagg caacgcggtt
     3901 ggttcgttta actttggaac cacgtggacc cggggcccgc tcgacaattc ggcagccgcg
     3961 ccaacgggac agggcttggc atccttcctt atgggtttgc ccgacggcgg caatgcggac
     4021 atcaatgcaa gctacgcgga gcagagcgtc atgtcgtcgc tgttcatcca ggaagactgg
     4081 aaagctaccc ggcgtttgac gctcaacttc gggcttcgtt atgaactcga aacgccgatc
     4141 accgagcggt tcaaccgcac cttgcgcggg tttgactatg acgccccgag cccgatcgaa
     4201 gccgccgcgc gcgaggtgta tgcacgttct cccatcccgg aagtcccggt ttccgagttc
     4261 cgtgttcgcg gaggactgct gttcgccgga cagaacggtg tccctcgcgg actttgggag
     4321 atggataaga acaacctcca gccgaggatc ggattcgctt accaactccg cgacgcgacc
     4381 gttctccgcg gaggatacgg aatcttccac gactttctgg ggcttcgtgt tggaactgtc
     4441 gacgtgatcc agtccggtta cagccggcgt accgatttcg ttgcttcgct cgacaacggc
     4501 cagaacttca tcgccaatct gacgaatccg ttcccgaacg ggctgctcga gccgctcggc
     4561 tcatcgctcg ggctttcagc aaatctcggc caggcggtga cgttcctccg tacgaacccg
     4621 aaaacgggat acatgcagcg ttggtctttg aacgtgcagc atgagatacc gggcaatgtc
     4681 ctgatcgaca taggctatct cggcaatcgc gggactggct tgcccatgat tcgcgaactg
     4741 aactatgttc cgaatcaata cctgagtaca cttccagtgc gcgacgaccc agcgaacaat
     4801 ttcctcacag cgcaggttcc aaacccattc gccgggctgc tgccggggac ttcattaaac
     4861 ggaaacaccg tttcccgttc agttttgttg accgggtatc cgcacttttc ttcggttcgc
     4921 cggaacatcg aatcgatcgg ttactcctgg taccactcac tgcaaacgcg tgtggagaaa
     4981 cggcttggtt ccggcctcgt gatgggcgga gcgtggactt ggtcaaaggc cctggctgcc
     5041 atgagctttc gcaatgcggg cgacccgttc ctggaagaag tgctgtcttc gcaggaccgg
     5101 actcaccggc tggcggtcaa ctgggtttac gacctgccga taggacgggg acgccgcata
     5161 ggatccaatt tcaacgctgt tgccgatgct attcttggcg gctggcaggt tgcgggtatc
     5221 taccaggccc agagtggtgc tccgctaggc atcggcgatc cggtcctcac cggacctttc
     5281 agcgtgaccg atttgatcct gccggaagac cagcgcatac ccgagcgcta tatcaacacg
     5341 gatgttgacc tcaataagct gccggcgcgc gctttcgtgt cgcacctgaa cacgatgtcc
     5401 tcacgcttcg gagcgcttcg gtccgatggc atgaaccagt gggacatttc tgtagcaaaa
     5461 acatggaggc ttaccgaaag tgtccagttg cggttccaga cacaattcat caatgccttc
     5521 aatcacgtga cgttcagcgc gccgaactta acctcgacca acaccgcgtt cggaacggtc
     5581 acgagcgaag ccagcatgcc gagaacgata aggtgggggt tgaggatcga gtactgaacg
     5641 gactatgaat catcgtctat ttgcaatcgc tgtcctcgcg ccggcactgc tggcacagca
     5701 ggcagcggtc gtgccgcccc agtatccggt ggcttccaat cctgacttcc tgctgaataa
     5761 gccgtcggag gatccactga cgaagcggta tgcctggatt ccgaagccgc tctactacgg
     5821 aggcaaggac accgtcgggc ctctcgacgc cgattgggta tggcccgaca tcgcgctcgt
     5881 cgattggaac cgggacggcc tcctggacct ggtctgcgcg ctgacggcgg gtacttactc
     5941 gcaccggccg aaagaccaac agttccgcgc gctagtgtat cgaaacaccg gacagcgcga
     6001 gggccgcacg ccggtcttcg acgaaccata ccaggtggac ctcagctttc cgatcgggtc
     6061 gatggtcttc gacgacatcg ataacgacgg gacgctcgat ctgattatgt ggtcgcgcaa
     6121 cctgtttcgc tggtatcgcg atacttcgaa gtcgggcgag cgccgctttg aatttgccgg
     6181 gaatcttcag gactatggtt tgcacactga tttgaagctt ctcgaaagcg tcagcatgtc
     6241 tccatcgctt cagttggtgg attgggatgg ggacgggcgc aaagacctga tcgttggcgg
     6301 gcgcggtggc aacgtcaact attatccgaa gctggaagtc gggttcggaa agggcttcgc
     6361 tccagacggt agttggctag gcggagaccg taatggttcc gtcgtcttcc acaaaaatct
     6421 cggggtgcgt gatggcaagc tcaccttcag taccggtaag cgccttacgg ccggcgagga
     6481 cgaacgtgcc atttcgtatt tcgacaccgc caccgcagcc gtcaccgact acaacgatga
     6541 tggtcttcca gacatcgttg tcgccagctt cgacaatctt ttctggtatg agaacgtcgg
     6601 cgcgccgggc cggcctatgc ttatctcggg caaaaggatc ccggtggctg ccggtgagag
     6661 gctgccttgg gagcgcgtgt tgccaatcgt ggcgaactgg aactcctcag atcggaacca
     6721 tctgatactc cagggatcga gctatgcctg gtacctgcca aattccggca ataaaggcat
     6781 gccccggtat gaggcgatca cgcaactgct tcaacgcaac ccacccgtcg gggccggcga
     6841 tttcgcaatc gtggctgccg gcgatctgaa cggagatggc aaaccggact tggtgatcgg
     6901 caacgaggat ggctacctgc tgtacgtcga aaacacctcg aaaggaaacg agccggagac
     6961 cttcaagccg gccgtgcaat tgaaatcggc aggcgaagtc ttccgcatag agtcgggccg
     7021 cgggctacag ggccccgcgg aggcgcgatg gggatacacg attcctgttt taaccgactg
     7081 ggacaacgac ggggatctcg acttgattgt aggcagtcta cacagctact acctgctgct
     7141 cgaaaataca ggcacgagat ccacacctgt gtttgggaaa ccggagcctc tgacgagcgg
     7201 cggggaaacc cttctgctcc cctggcgcac acgaccggtc gttcacgatt tcgacggtga
     7261 cggccaaaaa gatctgctcg cgctggatcc ggagggcatg ttgaccctgt atcgccgttc
     7321 cggcgcgcgc gaggtccagg ccggcaaacg cattctcgat cagaataatt cccccatcaa
     7381 gctcgacggc aaaggccgcg agacaggccg cgcgatgctt gccgtgatgg actgggatga
     7441 ggacggcaaa ctggacctga tcgccggtaa cgcgatcgaa aacttcgatg gactgcgctg
     7501 gtaccgcaac atcggaacaa atacgaagtg ggtgctggcc cggcaaccca acctggcgct
     7561 aaatcttccc tggaaccact accaccaaat cgagccggtc gactggaatc gtgacggcaa
     7621 actcgatttg atcgcgggaa gtgaaggtgg ctgggtttat tactacccgc agaggtgatc
     7681 tatgaccaaa ttggcaggac tgtacccggc cgtcgtgacg ccgttcaggg acgcgcgtag
     7741 cattgatctc gatgcacttg ccgaaaacgt tcaacggctg ctggcagccg gtatggacgg
     7801 agtatatgtc ggcggcaaca cg
//
LOCUS       DASTMK010000242         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_139217, whole genome shotgun sequence.
ACCESSION   DASTMK010000242 DASTMK010000000
VERSION     DASTMK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139217"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFQ79_10545"
     CDS             complement(<1..964)
                     /locus_tag="VFQ79_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140143.1"
                     /translation="MLRFLPFLVCLVSQVSAGQGPFASARDASEYARRVKVVRAIPGF
                     VALWDFVLREPGDGRFLAHQAAGVRHNFALEAVNYVRDYWNEGRAATYNDFPLLNRGP
                     FGQAVRFLPEPETTFRPTLLVPRARLHDSGLDVKGPGRSVSLAAWIIRESGNHAIAGI
                     WHEGTDLRDIASPARRVERGKRQYALFAGLAANNGASAAHVSENGAKSFGDRYARNLA
                     VTPDVIPTVQPGATVEALDRMWSVAGFVFDNEKNTVTAYLNGNAKEYWIDNPASHPFF
                     QWPAKGWLQAQLRKMPGTQPGEDPEFPADQFYTPPETKLRERRIL"
     gene            complement(971..2341)
                     /locus_tag="VFQ79_10550"
     CDS             complement(971..2341)
                     /locus_tag="VFQ79_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0140144.1"
                     /translation="MPQYRIAATFVLAACSAFGGGMLYAASNTDAPAGWITGSPRKQI
                     EPRFSYDAKGGREGAGAMIIEADAREGLDGFWSHTYPVTGGKFYEFAVFRRISDVEWP
                     QQNGVVTIRWTDANGKGVLDDGPLVEGYLKRFTPIAPREYPRDEQRGSDGWTRVSGTY
                     RAPATAKAAIVQLHLRWARNARVEWSGATLREVDTPQPRKVRLAAIHLQPGGKTPDGN
                     RKAYAPLIREAGRQKADLVVLGETITYFRTGLRPDEAAEPVPGPSTDYFGQLARENNL
                     HIVVGLYERVDHLVYNVAVLISPEGKLIGKYRKTTLPDGEAELGVAPGLDYPVFQTRF
                     GKVGMMICYDGFFPEVARELTKRGAEVIAWPIWGGNPDVARARAADNHVYLVSSTYED
                     VSSNWMFSAVWDHSGATPAIGKDWGTVVVAEVDLNEQTRWRSLGDFRSKLPRHVPLIQ
                     PETTFR"
     gene            2402..>3028
                     /locus_tag="VFQ79_10555"
     CDS             2402..>3028
                     /locus_tag="VFQ79_10555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0140145.1"
                     /translation="MPIDAGQERQFYDGWYAQFQTASDDALVCNLETFRRDISDPQSP
                     GFERRLLHQTALEHLLASSVAGYNVLDYCCGTGDWGLMLAGQGANVTLLDLSPVAIQV
                     ALRRAKLSGVEGRVRGVVADASNLACFRDGEFDLIFGSAAVHHTVKYPGAVEELVRVL
                     RPGGRLILAETYGNNRLLNLVRRLRWRISGLADDAGEDVLLSDSDLARL"
BASE COUNT          560 a          919 c          941 g          608 t
ORIGIN      
        1 ccagaatccg gcgttcgcgg agcttcgttt ccggcggcgt gtagaactga tccgcgggaa
       61 attcgggatc ctcaccgggc tgcgtaccgg gcattttgcg gagttgagcc tgtaaccatc
      121 ctttggcggg ccattggaag aaggggtggc tcgccggatt gtcgatccag tattccttag
      181 cgttgccgtt gaggtaagcc gtcaccgtgt tcttctcgtt gtcgaagaca aaacctgcca
      241 ccgaccacat ccggtccaag gcttccaccg tcgctccagg ctgcacggtg ggaatcacat
      301 ccggtgtaac ggcaaggttg cgagcgtacc ggtccccgaa cgatttcgcg ccgttttcgg
      361 aaacgtgcgc cgccgatgca ccgttattcg ctgccagtcc ggcaaagagg gcgtattggc
      421 gcttgccgcg ttcaacccgg cgggcaggcg aggcaatatc cctcagatca gtgccctcgt
      481 gccagattcc cgcgatggcg tggttgcccg attcgcggat gatccaggcg gccagggaca
      541 cgctcctgcc aggacctttg acgtcaagcc cggaatcatg caggcgggcg cgcggaacga
      601 gcagcgtggg gcggaacgtc gtttcgggct ccggcaggaa ccttacggcc tggccgaaag
      661 gcccccggtt cagcaacggg aaatcattgt aggtcgctgc gcgtccctcg ttccagtagt
      721 cgcgaacgta gttcacggcc tccagcgcaa agttgtgccg gacccccgcg gcctgatggg
      781 cgagaaaacg cccgtcaccg ggttcgcgca gaacgaaatc ccagagagca acaaagccag
      841 ggattgcacg aacgaccttc acccgcctcg cgtactcgga agcgtcgcga gcggatgcga
      901 agggaccctg cccggccgag acctgagaca ccaggcagac caggaatgga aggaagcgca
      961 gcacgcctac ctaccggaaa gtggtttccg gctggatcag cgggacgtgc cgcggcagct
     1021 tcgaacggaa gtcgccgaga ctgcgccagc gggtctgttc gttgaggtcc acttcggcga
     1081 ccacgacggt tccccaatct ttgccaatcg cgggagtggc tccggaatgg tcccagacgg
     1141 cagagaacat ccagttcgaa gagacgtcct cgtaggtgct gctgacgagg tatacgtgat
     1201 tatccgcggc tctggctcgc gctacgtcgg gattaccgcc ccagatgggc caggcgatga
     1261 cttcggcgcc tctcttggtc aactcgcgcg cgacttccgg aaaaaagccg tcatagcaaa
     1321 tcatcattcc gactttgccg aatcgcgtct ggaagacggg atagtccaat ccgggcgcta
     1381 cgcccagttc cgcttctcca tcgggcagag tcgttttccg gtacttgccg atcaacttac
     1441 cttcgggaga gatcaggacc gccacgttgt acaccaggtg atcaactctt tcgtatagac
     1501 ccacgacgat atgcagattg ttttcgcggg cgagctggcc gaaataatcg gtcgatggcc
     1561 ccggcaccgg ttcggcggct tcatcggggc gcaaacccgt gcggaagtaa gtgatcgttt
     1621 cgccgaggac taccaggtcg gccttctgcc tgcccgcttc ccggatgagc ggcgcataag
     1681 ctttgcggtt cccatccggc gtttttcctc caggctgaag gtggatggct gcgagccgca
     1741 ctttgcgcgg ttgtggagta tccacttcgc gcaatgtcgc accgctccac tcgacgcgcg
     1801 cgtttcgcgc ccaccgtaga tgcagttgca caatcgctgc tttggcggtt gcaggcgccc
     1861 gatatgtgcc ggagaccctg gtccaaccat ccgaaccccg ctgttcatcg cgtggatatt
     1921 cgcgtggagc tatcggagta aagcgcttca ggtagccttc caccagcggg ccgtcgtcga
     1981 gcacaccttt gccgttagca tctgtccagc gtatggtcac gacaccgttc tgctgcggcc
     2041 attcgacgtc cgagatacgc cggaacaccg cgaattcgta gaattttcca cctgtgaccg
     2101 gataggtgtg cgaccagaag ccgtccagac cttcccgcgc atccgcttca atgatcattg
     2161 ctccggcgcc ttcgcgccct cccttggcgt cgtaggaaaa ccggggctca atctgtttgc
     2221 ggggcgagcc ggtgatccat cccgcgggcg catcggtatt cgatgcggcg tacagcatcc
     2281 cgccgccaaa ggcgctgcac gccgccagga caaaggtcgc ggcaattcgg tactgtggca
     2341 tcagctatca cagtaacagt taagtggatc gcatcgggtc gcggtgctaa gattgtcccc
     2401 agtgccgatt gacgctgggc aggagcggca attttacgac gggtggtatg cgcagtttca
     2461 aactgcttcc gacgatgctc tcgtctgcaa tctcgagaca ttccgccggg atatctcgga
     2521 cccccaatct cccgggtttg aacggcgcct gctgcaccag acggctctcg agcatctgct
     2581 ggcatcctcc gtcgccgggt acaacgtgct cgattattgc tgcgggacgg gcgattgggg
     2641 actgatgctg gccggtcagg gtgcgaacgt tactcttctc gatctttctc cggtcgctat
     2701 acaagtggcg ctgcgaagag cgaagctgag cggggtggaa gggcgagtcc gcggggtcgt
     2761 agccgatgcg agcaacctgg catgctttcg ggacggcgaa ttcgatctca tcttcggtag
     2821 cgcggccgta caccacaccg tcaagtaccc gggagccgtg gaagaactgg tgcgcgtgct
     2881 gcggcccgga ggcagactga ttctggctga gacctatggc aacaaccggt tgttgaacct
     2941 cgtgcgccgt ttacggtggc gcatttccgg gctggcggac gacgccgggg aggatgtgct
     3001 gctttcggac agcgatcttg cgcggctg
//
LOCUS       DASTMK010000243         7969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_458625, whole genome shotgun sequence.
ACCESSION   DASTMK010000243 DASTMK010000000
VERSION     DASTMK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458625"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFQ79_10560"
     CDS             <1..889
                     /locus_tag="VFQ79_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0140146.1"
                     /translation="FTWISAAEGRKFQQVISEITESARALGPADHLKRREAAPVVPRT
                     VPAPVPPVVDEAGLRTNAAGLLRSGEAQVVIGYDAAGTPMFVRREEDCSRLAVNGNAR
                     VNLAGYLTRREVRSMGRPAVVALPSTTRAINILEQESQVDRSALVEIAVEKPSAGAPW
                     KELEDFLELAPEQRMAYWMEEFERCTRCYACRAACPMCYCERCVADKNRPQTIDTSAT
                     LKGNFAWHITRAFHQAGRCTGCGECTRACPAGINLGLLNKAVARAAFDNFGYQAGAER
                     GAGPLLGTYRPGDKEAFIR"
     gene            886..1863
                     /locus_tag="VFQ79_10565"
     CDS             886..1863
                     /locus_tag="VFQ79_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0140147.1"
                     /translation="MRVGREELCALAVDWLKSGKRVLAPRCVADGRVLYAAIQSAEQM
                     TLEGFLRPRNSIKEAVFPRHEALYRYRIDNGHIELTDALDAPEQVVFGARPCDAAALP
                     ILDAVFGWDSEDEFYKTRRQRTTVVTMACDTHDDSCFCTSVGLAPDATNGSDAMLYPA
                     GDGYEVAALTEKGRALFPDAGSVPGQKAEAPARQFDSAAVRAFVESRFEDPLWDEQAL
                     RCLGCGACAYTCPTCHCFDIVDERGARVRNWDSCQFALFTLHASGHNPRGSQPARQRQ
                     RVLHKFSMYPERFGSILCTGCGNCTRNCPVNLGLLPLLKAIDHAQPLQA"
     gene            1841..2674
                     /locus_tag="VFQ79_10570"
     CDS             1841..2674
                     /locus_tag="VFQ79_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEU0140148.1"
                     /translation="MRNLYKPDLMTVTGVCRHTPDVKSVRVEFREPARARDFSFHVGQ
                     FGMFSVFGSGEATFNICSSSNWHDFVEFCFRKTGRVTEALWEIEPGDTIGFRGPYGNW
                     YPMEKWEGRDLIFLGGGIAMPPIRCAIWYALENRSRYGNITIVYGARTVGDLVYVQEL
                     DEWAAQERVRVIRCVDPGGETPDWKGEIGFVPAVFERSGVPANNAAALVIGPPIMIRN
                     TLPVLERSGMHPRDIFTSLENRMKCGVGKCGRCNAGPVYVCKDGPVFSMEELSRIPAD
                     Y"
     gene            2797..2994
                     /locus_tag="VFQ79_10575"
     CDS             2797..2994
                     /locus_tag="VFQ79_10575"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEU0140149.1"
                     /translation="MKLRYDPDVDVLRIILSDAPVEESDEDKPGVILDYDRNGNIVGL
                     EILDASRRMDNPMSVEYAVTR"
     gene            3383..5734
                     /locus_tag="VFQ79_10580"
     CDS             3383..5734
                     /locus_tag="VFQ79_10580"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0140150.1"
                     /translation="MPFLFFILFAAAGMVLQAADEFVLLQSGVLRDQQRVDSFEMQKR
                     PLTNAEYKLFIDATGHAAPLHWENGRIPAGMDNHPVIFVNRIDAAAYLKWRSEKERRI
                     YRLPTALEFEYAARAGEAEATYPWGSADPAGKANFDPKGDRTFGEWRTYVKPAGSYPP
                     NKWGLYDMAGNVWQMVDRYPDPAISRFVYRIENPTERENQLAGGSWARASYYLRCGVF
                     GGASSGIRHPDIGFRLVREPEGSTHFRRVPRRVVAAPLGGGKVFLSWQLLPEDTAATG
                     FHVYRATRPDAAGERITSSAIPHSTSFTDASAPAVSRLYYRVRPVQGGGKEGPPSEWA
                     GVDPGANRSNAVAVFEPTVQEGGAVPIFGDLNGDGVLDAVLRLDNGIREMSRDPGVPV
                     ELEAFTSYGRSLWRRALVRHDHCFGSANNVPVVVYDLDGDGKAEVVSRYQEGEQVYLA
                     VLDGMSGRVLRKTPWTEMASDFARSSTRIHMSIAYLNGKTPAIVTQTGLYENEIFDAY
                     DSELNKLWQYRSFAETNGSGSHHIDIADVDGDGKDEVFNGTTVLNPDGTLRWSIYREH
                     PDIVAIKRILPGSKERQVYYAVESGVHAGAYLVDAKSGKILWKTNREDDPRWVHAHIG
                     WASDIFRGSPGMEMLTNRDGHLAKDQVLFASDGRILMNPFPPGWKPVHWTGDETRELM
                     SNDGTRLGRFTGKTVEAIETAVPNELGSGGCNMVADLAGDYRDEVVCTGKTNEGRRAV
                     FVFTNVEPLAKREVTRTANREYRLWMARNQGGGYASYFEWQPE"
     gene            complement(5736..7595)
                     /locus_tag="VFQ79_10585"
     CDS             complement(5736..7595)
                     /locus_tag="VFQ79_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEU0140151.1"
                     /translation="MAARYLPLLLFAVVPLFASDWRYYGSDSGGTKYSSEKQINRTNV
                     SRLKVAWTYHTGDMSDGTTYVSRSAFEATPLVVDGVMYFTTPFNRLIALEAESGRELW
                     SFDPELDKERPVNLFIHRGAAWWTDGKAARVVFGTLDGRLFSIDAANGKPDPAFGEKG
                     VVDLRKGVADRFPGRGYGMTSPPAIYKDLVICGALAADGESRGPAGDIRAFNVRTGRL
                     VWTFHAVPRPGEFGNDTWEGESWKDRAGANAWSMLSVDEDRGIVYVPLTSPATDLYGG
                     DRKGAGLFGDSLVALDASTGKRLWHFQTIHHDIWDYDLPAQPVLTTVVRNGKKVPAVA
                     QITKTGFTFVFDRYSGKPLFEIEERAVPASDVPGEEAYPTQPFPLKPPPFARQSFRPE
                     ELTRVTPETRAFCEKLMKDAVFGTLYTPIGLKPTVLFPGTNGGANWGAGSFDPETGTL
                     YVNSMDVGMVYQMVERPQGSEIPFRPRGMGSPNSRFWDKNQYPCQEPPWGHLTAIDLN
                     TGEFRWRSVLGVVDKLIDKGLPPTGAPNLGGSIVTAGGLVFIAATNDSRFRAFDKDTG
                     KELWSTRLPASGHATPMTFVGKKSGKQFVVIAAGGGNKYNETYSDALVAFALP"
     gene            complement(7598..>7969)
                     /locus_tag="VFQ79_10590"
     CDS             complement(7598..>7969)
                     /locus_tag="VFQ79_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0140152.1"
                     /translation="FTSPEDAARQLETNPVDVIVTDYLMPKMNGIQLLGKAKNLQPEA
                     ARVLLTGHADKQSAIQAINEIGLYQYLEKPWDNAQLLLVIQGAIERTRLLRTLRDKIS
                     ELDAAHSNLKDVQTRLLRAFL"
BASE COUNT         1485 a         2365 c         2647 g         1472 t
ORIGIN      
        1 cttcacgtgg atttcggccg ccgaaggcag gaagttccaa caggtgatct cggagataac
       61 ggaatccgcg cgggcgctgg gtcccgccga tcatctgaaa cggcgcgaag cggctccggt
      121 tgtgccgcgc accgtgccgg cgccggttcc gccggttgtg gatgaagccg ggttgcggac
      181 gaacgccgcc ggcctgctgc ggtccggaga agcgcaagtg gtgatcggct atgacgcggc
      241 gggaaccccg atgttcgtgc ggcgcgagga ggattgctcg cggctggccg tcaacgggaa
      301 tgcgcgcgtg aacctggccg gttatctgac gcggcgggaa gtgcgctcga tggggcggcc
      361 cgcggtggtg gcgctgccgt ccactacgcg cgcgatcaat attctggaac aggagtctca
      421 ggtagaccgt tccgccctgg tggagattgc cgttgaaaag ccctccgcgg gagcgccctg
      481 gaaggagttg gaagattttc tcgaactggc gccggagcag cggatggcct actggatgga
      541 ggagttcgag cggtgcacga ggtgctatgc gtgccgcgcc gcctgcccga tgtgctactg
      601 cgagcgatgc gtcgcggaca agaaccgccc gcagacgatc gacacttcag cgacgctgaa
      661 aggcaacttc gcctggcaca tcacgcgggc ctttcaccag gcgggccgct gtacgggttg
      721 cggcgaatgc acacgggcgt gtccggcggg catcaacctg ggcctgctga acaaggcagt
      781 tgccagggcg gccttcgaca acttcggcta ccaggcgggc gcggagcgcg gcgcagggcc
      841 gcttctcggc acctatcgcc ccggcgacaa ggaggcgttc atccgatgag agtggggcgg
      901 gaggagttgt gcgcactggc ggtcgattgg ctcaaaagcg gcaaacgtgt gctggcgcct
      961 cgctgtgtgg cggacggccg cgtgctctat gccgcgatcc agagtgcgga gcagatgacg
     1021 ctggagggtt ttctgcggcc gcgcaactcc attaaagaag cggtgttccc gcgtcatgag
     1081 gcgctctacc gctatcggat cgacaacggc catatcgagc tgaccgacgc tctcgacgcg
     1141 ccggagcagg tggtgttcgg cgcgcggccc tgcgatgcgg cggcgctgcc gatcctagat
     1201 gcggtttttg gctgggacag cgaagacgaa ttctacaaga cgcgccgcca gcgcacgact
     1261 gtcgtcacca tggcgtgcga cacgcacgac gattcctgtt tctgcacgtc ggtggggctg
     1321 gcaccggatg ccacgaacgg atcggacgcg atgctttacc cggcaggcga cggctacgaa
     1381 gtggcggctc tgaccgaaaa agggcgtgcg ctcttccccg atgccgggtc cgttcccggg
     1441 cagaaggccg aggcccccgc gcggcagttc gattcggcgg cggtgcgcgc gtttgtggag
     1501 tcgcgtttcg aagacccgct gtgggacgaa caggcgttgc gatgccttgg atgcggggcg
     1561 tgcgcctata cctgccctac gtgccactgc ttcgacattg tggatgaacg tggcgcgcgc
     1621 gtgcgcaact gggattcgtg ccagttcgcg ctctttacgc tgcatgcctc cgggcacaac
     1681 ccgcgcggca gccagccggc gcggcaacgc cagcgcgtgc tccacaaatt ttccatgtac
     1741 ccggagcgct tcggctccat tctgtgcacg ggatgcggca actgcacgcg caactgcccg
     1801 gtgaatctcg gcctgcttcc gctgttgaag gcaatcgatc atgcgcaacc tctacaagcc
     1861 tgacctgatg acggtgaccg gcgtttgccg ccatacgccc gacgtgaaga gcgtccgcgt
     1921 cgagttccgg gagccggcgc gtgcgcgcga tttttctttt catgtgggcc agttcggcat
     1981 gttctctgtg ttcggttcgg gagaggcgac gttcaatatc tgctccagtt ccaactggca
     2041 cgacttcgtc gaattctgtt tccgaaagac cgggcgcgtg acggaagcgt tgtgggagat
     2101 cgagccgggc gacacgatcg gattccgggg cccttacggc aattggtatc cgatggagaa
     2161 gtgggaaggc cgcgacctga tctttttggg cggcggtatc gcgatgcctc ccatccgatg
     2221 cgctatctgg tacgcgctgg aaaaccgctc ccgctacgga aacatcacca tcgtctatgg
     2281 agcgcgcacg gtgggcgacc tggtttatgt tcaggaactc gacgagtggg ccgcgcagga
     2341 acgtgtacgc gtgatccgct gcgtggaccc gggcggtgaa acgccggact ggaagggaga
     2401 gatcggcttt gtgccggcgg tgttcgagcg ctccggggtt cccgccaata acgcggccgc
     2461 actggtaatc ggccctccca tcatgatccg gaacactctg ccggtgctgg agcgcagcgg
     2521 catgcacccg cgcgacattt tcaccagtct tgaaaaccgc atgaagtgcg gcgtcggcaa
     2581 atgcggccgg tgcaacgcgg ggccggtgta cgtgtgcaaa gacggtccgg tcttcagcat
     2641 ggaagagttg tcgcggattc cggcggatta ttagcgtggg ccgccacttg ctgacgcgcg
     2701 cggctcggtt cctgagcggt gacagtcagg aagtgcgcga atatcctcgg cagtgtgtta
     2761 cgcttgcgca acgatcgaaa aatactggag gatggaatga agctcaggta cgatcccgat
     2821 gtggatgtgc tccgcatcat tctcagcgat gcgccggtcg aggagagtga tgaggacaag
     2881 cctggcgtta ttctcgatta cgacaggaac ggcaacatcg tcggtctgga aatcctggat
     2941 gcctcccggc gcatggataa ccccatgtcg gtcgaatacg cagtcacacg gtagcggcgc
     3001 ctcatggccg cctgcctccg cttgctggcg cgcgcggctc ggaaacatgc gcggatcggt
     3061 aacgtttgag gtgcgcattt gcccgcgcac cgggaccggt ccctgtcccc cgcgcaaagg
     3121 tgtaggggga cttaggcagg cctgtcacgt tgacgcgccg cggtctcagg ttttacactt
     3181 cccggggtag tcctgcgcgg gtctcggtgg gttgcctctc ggccaacgag caactcctcg
     3241 ctcataacat acaccggcgt tcgaagcggc ggtgtgcgcg gatgcgcgga gatgcctgaa
     3301 aagaaacggt gaaatgcacc gtggcatgtg tgtgaacgcc ttaaggttgc gcgccgccgc
     3361 cttgcctgct aagctgccgc cgatgccatt tttattcttc attctgtttg ccgccgccgg
     3421 gatggttctg caggcggccg atgagtttgt tctccttcaa agcggagttc ttcgggatca
     3481 gcagcgggtc gacagcttcg aaatgcagaa gcggcctctc acgaacgccg agtacaagct
     3541 tttcatcgat gccacgggcc atgccgctcc gctgcattgg gagaacgggc ggataccggc
     3601 gggaatggat aaccatcccg tcatcttcgt gaaccggatc gatgccgccg cctatttgaa
     3661 gtggcgcagc gaaaaggagc gccgtatcta caggctgccg acagcgctcg aattcgaata
     3721 cgccgcacgc gccggcgagg cggaagcgac ctatccatgg ggcagcgcag acccggccgg
     3781 caaagccaac ttcgatccaa agggggatcg cacgttcggc gaatggcgca cctacgtcaa
     3841 accggccggg agttatccgc cgaacaaatg gggtctgtac gacatggcgg gcaatgtctg
     3901 gcagatggtc gaccggtatc cggacccggc catctcccgc ttcgtgtacc gcatcgagaa
     3961 tccgacggaa cgggaaaacc agctcgcggg aggatcgtgg gcgcgcgcaa gctattacct
     4021 gcgatgcggc gtcttcggcg gagcttcctc cggaattcgt caccccgata tcggcttccg
     4081 gctggttcgc gagccggaag gctccacgca cttccggaga gttccgcgcc gtgtcgtcgc
     4141 cgcgccgctt ggcggaggaa aagtatttct gagttggcaa ctgctgccgg aagacaccgc
     4201 cgctaccggc tttcatgtct accgcgccac gcggcccgac gcggcgggcg agcgcatcac
     4261 atcctcggcg attccccatt ccaccagctt cacggatgcg agcgctcccg cggtgagccg
     4321 gttgtattac cgcgtgcgtc ccgtgcaggg aggcggcaag gaaggtccac cttcggaatg
     4381 ggccggcgtc gacccgggcg caaaccggtc caatgccgtc gctgtctttg aacccaccgt
     4441 acaggaaggc ggagcggttc cgattttcgg agacctgaat ggagacggcg tgctggacgc
     4501 cgtactgcgg ctcgacaacg gcatacggga gatgtcgagg gaccccggcg taccggtgga
     4561 actcgaagcg tttaccagct acggacggag cctgtggagg cgcgcgctgg tccggcacga
     4621 ccattgcttc gggagtgcga acaatgttcc ggtggtggtc tacgacctcg atggcgacgg
     4681 caaagcggaa gtggtgagcc gctaccagga aggggaacag gtttatctcg ccgtgctcga
     4741 cggcatgagc ggccgcgtgc tacggaaaac gccctggacg gagatggcgt ccgactttgc
     4801 gagatcgtcg acgcggattc acatgtccat cgcctacctc aacgggaaga cgccggccat
     4861 tgtcacgcag acgggcctct atgagaacga gatcttcgat gcctacgatt cggaactgaa
     4921 caagctgtgg cagtatcgca gctttgccga aaccaacggc agcggctcac accatatcga
     4981 tatcgcggat gtggacggcg atggaaaaga cgaggtcttc aacggcacga cggtgctgaa
     5041 cccggatggc actctccgct ggtccatcta ccgggagcat cccgatattg ttgccatcaa
     5101 gcgcatcctg ccgggttcga aagagcgcca agtgtattat gccgtggaaa gcggcgtaca
     5161 tgcgggcgcc tacctggtgg acgcgaaatc gggaaagatc ctctggaaga cgaaccgcga
     5221 ggacgacccg cgctgggttc atgcccatat cggctgggct tccgacatct ttcgaggctc
     5281 gcctggtatg gagatgctga cgaaccgcga cgggcacctg gccaaagacc aggtgttgtt
     5341 cgccagcgac gggcgcattc tgatgaatcc gtttccgccg ggatggaagc cggttcactg
     5401 gaccggcgat gaaacacgtg agctgatgtc gaacgacggc acgcggctgg gccggttcac
     5461 gggcaagacg gttgaggcga tcgagacggc tgtacccaac gaactgggca gcggcggctg
     5521 caacatggtg gcggacctcg ccggcgatta ccgcgacgaa gtcgtctgca ccgggaaaac
     5581 aaacgagggc aggcgggcgg tgttcgtctt cacgaacgtg gagccgttgg cgaagcgcga
     5641 ggtcacgcgg acggcaaacc gcgaataccg gctctggatg gcgcggaacc agggcggcgg
     5701 atacgcctcg tatttcgaat ggcagccgga gtagctcagg gcagcgcgaa cgccaccagg
     5761 gcgtccgagt aagtctcgtt gtatttgttc ccgccgccgg cggcgatgac gacgaactgc
     5821 tttccggatt ttttgccgac gaaggtcatc ggcgtggcat ggccgctggc gggcaggcgc
     5881 gtactccaca gctccttgcc ggtgtccttg tcgaaggcgc ggaaacggct gtcgttggtg
     5941 gcggcgatga acaccagccc gcccgcggtg acgatggagc cgccgaggtt cggcgcaccg
     6001 gtagggggca ggcccttatc gatcagcttg tccaccacac cgagcacgga acgccaacgg
     6061 aattcgccgg tgttcagatc aatcgccgta agatgtcccc agggcggctc ctggcaaggg
     6121 tactgattct tgtcccagaa gcgcgaattc ggactgccca ttccccttgg gcgaaacgga
     6181 atttcggagc cttgtggacg ttcgaccatt tgatacacca tgcccacgtc catcgagttc
     6241 acgtagagcg ttccggtctc gggatcgaac gatccggcgc cccagttcgc ccctccgttc
     6301 gtgcccggaa acagcaccgt gggcttcagc ccgatcggtg tataaagcgt gccgaacacc
     6361 gcatccttca tgagcttttc gcagaaggca cgcgtctcgg gcgtgacccg ggtcaactcc
     6421 tcgggacgaa aactctggcg ggcaaacggc ggaggtttca gaggaaacgg ctgggtgggg
     6481 taagcctctt cgcccggtac gtcgctggcg ggaacggcgc gctcttcgat ttcgaacagc
     6541 ggctttccgg aatagcggtc gaacacgaac gtgaatcccg ttttggtgat ctgggcgacg
     6601 gcgggaacct tcttgccgtt gcgaaccacg gtggtgagaa ccggctgcgc gggcagatcg
     6661 taatcccaaa tatcgtgatg gatcgtctgg aaatgccaga ggcgtttgcc ggtggaggca
     6721 tcgagcgcaa cgagcgaatc gccgaacaag cccgctccct tccggtcgcc gccatataaa
     6781 tccgtagcgg gcgaagtgag cggaacgtag acgataccgc ggtcttcatc gacgctcaac
     6841 atggaccagg cattggcgcc ggcccggtct ttccaggact ccccttccca ggtgtcgttg
     6901 ccgaactcgc cggggcgcgg cacggcatga aacgtccaca ccaggcgtcc cgtgcgcacg
     6961 ttgaaagcgc ggatgtcgcc ggcgggtccg cgggattcgc cgtccgcggc aagcgcccca
     7021 caaatgacga ggtccttgta aatggcgggg ggcgaggtca tcccgtagcc gcggccggga
     7081 aaacgatccg ccacgccctt gcgcaaatcc acgacgccct tctcgccgaa agcgggatcg
     7141 ggcttgccgt tggccgcatc gatcgagaac aggcgtccgt cgagagtgcc gaaaaccacg
     7201 cgggccgctt tgccgtccgt ccaccaggcg gctccgcgat ggatgaagag attgaccggg
     7261 cgctctttgt cgagctccgg gtcaaaggac cacaactcgc gccccgattc agcctcgaga
     7321 gcgatcaggc ggttgaacgg cgtggtgaag tacatgacgc cgtcgaccac caggggagtc
     7381 gcttcgaacg ccgagcggga aacataggtc gttccgtcgc tcatgtcgcc ggtgtgatag
     7441 gtccaggcga ctttcaggcg cgaaacgttc gtgcgattga tctgcttttc gctggaatat
     7501 ttcgtccctc cgctatcgct cccgtagtag cgccaatcgg aagcgaacaa gggaacgacc
     7561 gcgaacagca gcagtgggag atatcgggca gccatcacta caggaatgcc cgcagaagcc
     7621 gggtctgcac gtccttgaga ttcgagtgcg cggcgtccag ttccgagatc ttgtcgcgca
     7681 aggtgcgcaa cagcctggtg cgctcgatgg cgccctggat caccaacaaa agctgcgcat
     7741 tatcccaggg cttctcgagg tactgataga ggccgatctc gttgatggcc tggatggcgc
     7801 tctgtttgtc ggcgtgtccg gtcagcagaa cgcgagccgc ttccggctga aggtttttgg
     7861 cttttccgag caactggatg ccattcattt tcggcatcag gtagtccgtg acgatgacat
     7921 cgacgggatt cgtctccaat tgcctggcgg cgtcctccgg gctggtgaa
//
LOCUS       DASTMK010000244         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_491973, whole genome shotgun sequence.
ACCESSION   DASTMK010000244 DASTMK010000000
VERSION     DASTMK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491973"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFQ79_10595"
     CDS             complement(<1..1163)
                     /locus_tag="VFQ79_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0140153.1"
                     /translation="MRKGKLAALLGVLSLAPAAFAQIIAFENVNIVPMDRERVVETQT
                     VIIRDGRIAQIGPANRVKAPEGAERINASGKYLMPGLAEMHGHLPSSDAPRSLVEHFL
                     FLYIANGVTTVRGMQGNPVALQIREDVNAGKLPGPRLYVAGPPFGGNVRTPEIAEKMV
                     REQKAAGYDLLKISEGMGPEAYDAVVRTARELQIDFAGHVPNEVGVRRVIQARQRSID
                     HLDNYIEGLESETSPLRSQDPKVRARDLPFHVDEGKIAELVRLTREAGVWNMPTMACW
                     ENFYSADDGEKLRSSIPEVRYMPRSAVDNWVKGKNSLLRPDGGPFLGFLVGGKTGPRV
                     MDLRRKILRALHEAGAKIALGTDSPQIFSVPGFSIHREMSLMAQCGMKPFEVLR"
     gene            1314..2009
                     /locus_tag="VFQ79_10600"
     CDS             1314..2009
                     /locus_tag="VFQ79_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0140154.1"
                     /translation="MKVGIIGAGQIGGTLTRRLASLGHEVFVANSRGPETLAGLASET
                     GAKAVTVPEAARAGEVVIVTIPEGKVRDLPKDLFAGVPENVVVVDTGNYYPRERDGRI
                     DEIEDGMMESRWVEHQLGRPVIKAFNNIYASHLMEYGRPAGSPNRFALPVAGDDDRAK
                     KVVIGLLNELGFDGVDAGGIDESWRQQPGTPVYGADLDAAGVRRALAEAKSERTPQWR
                     ATNNSPGAFGRPA"
     gene            2046..2528
                     /locus_tag="VFQ79_10605"
     CDS             2046..2528
                     /locus_tag="VFQ79_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEU0140155.1"
                     /translation="MTLPGLLHAFLIGGVSGLRAMTAPAIVSWGAHLGWIDLSQTPLA
                     FLGYAATPYIISLLAILELIADKLPSTPSRKAPPGFIARLGLGTFSGYALSLGIGQSG
                     VIGAVLGFAGAIAGTLGGYEARTRSVKALKVPDIVVALLEDAIAIAIGLAAVHHNQPL
                     "
     gene            2601..>3708
                     /locus_tag="VFQ79_10610"
     CDS             2601..>3708
                     /locus_tag="VFQ79_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0140156.1"
                     /translation="MKSSASFMEAVFVAAASLRSSKLRSFLTLLGIILATTTLIAVMG
                     IIHGMDVYIATQVSDMGVDGYRVRRVVMMGERDPKRWLQLNQRNPQLTPEEFEFLRER
                     VTLSREIGMDASRGVTVTSKGVEIDGAQLQGVSPSVGVISNVPVETGRFLSDSDNRRR
                     LSVAVIGSDVAEKFFAGVDAIGKTIDVEGRPFEIIGTCKSRGSVFGQSQDNFVMIPVE
                     TYFKMYGSRSGVSFSALALDRNHLEQAQDEVRALLRAFRHLRPGEEDNFGIMASSSLV
                     DMWDRMTGAIAATAVGVVSVFMVVGGVVIMNIMLAVVTERTHEIGIRKSVGARRRDIL
                     NQFLVESSMLSAAGGFIGVMVAWFSAIAVRRLTPV"
BASE COUNT          712 a         1080 c         1151 g          765 t
ORIGIN      
        1 cgcagaactt cgaagggctt cattccgcac tgcgccatta acgacatctc acggtgaatc
       61 gagaatccgg gcacgctgaa aatttgcggc gaatccgtgc ccagcgcgat cttcgctccg
      121 gcttcatgca gcgcccgcag aattttccgg cgaagatcca ttacccgcgg accggtcttg
      181 ccgccaacca ggaagcccag aaacgggccg ccatccggcc gcaatagtga gttcttccct
      241 tttacccagt tgtcaacggc ggagcggggc atgtagcgca cctccggtat ggaagagcgc
      301 aatttctcac cgtcatcggc gctgtagaag ttttcccaac acgccatggt gggcatattc
      361 cagactccgg cttcgcgtgt gagccggacg agttccgcaa tcttgccttc gtcgacgtga
      421 aagggcagat cgcgggctcg tactttcggg tcctgcgagc gaagcggcga tgtctcggat
      481 tcgagcccct cgatgtagtt gtcgagatgg tcaatgctgc gctggcgcgc ctgaatgacc
      541 cgccgcacgc ccacttcgtt cggtacgtgc ccggcgaagt cgatctgcag ttctcgcgct
      601 gtgcggacca cagcgtcgta cgcttccggg cccattcctt cagagatctt cagcagatcg
      661 tagccggcag ccttctgctc gcggaccatt ttttcggcta tctccggcgt ccggacattg
      721 ccgccgaacg gcggcccggc gacgtagagc cgcggtccgg gtagtttgcc ggcattcaca
      781 tcctcccgga tctgaagagc aacgggattg ccttgcattc cccggacggt ggtgacgcca
      841 ttggcaatgt agagaaaaag gaaatgctct acaagactcc gcggcgcgtc ggaagaaggc
      901 agatgaccgt gcatctccgc aaggccgggc atcaggtatt tgccggacgc gttgatccgt
      961 tcggcacctt ccggagcttt gacgcgattg gccggcccaa tctgcgcgat acgcccgtcg
     1021 cggataatca cggtctgggt ttcgacgacg cgctcgcggt ccatcgggac gatgttgacg
     1081 ttctcgaacg cgatgatttg ggcgaatgca gcgggagcaa gtgaaagcac cccgagtaat
     1141 gccgcaagct ttccctttcg cataatggtt ccggttcccc cctggatgac tgttcgtata
     1201 gtgcgacggg cacaggccgt tttcgtttcg ccgcaggtgg gagcggcgcg gaaatccgca
     1261 acacatccgc gctttccgca tcctaataga ggagcacata gagaggagta gacatgaagg
     1321 ttggcatcat tggcgcaggg cagatcggcg gcacactcac acgccggctg gcctccctgg
     1381 gacacgaagt atttgtagcc aattcgcgag gacccgaaac gctcgccggt ctggcaagcg
     1441 aaactggtgc gaaggcggtc acggttcccg aagccgcccg ggccggcgag gtggttatcg
     1501 taacgattcc cgaggggaaa gttcgagacc tgccgaagga tctgtttgcc ggtgtccctg
     1561 agaatgtcgt ggtcgtggac accggcaact attacccccg ggaacgggat ggccgtatcg
     1621 acgaaatcga agatggaatg atggaaagcc gctgggttga gcatcaactc ggccggcccg
     1681 tgatcaaggc gttcaataac atctacgcca gccatctgat ggagtatgga cgcccggcag
     1741 gcagcccaaa ccgcttcgcg ctgcccgtgg ccggagacga cgaccgggca aaaaaagtcg
     1801 tgatcgggct gttgaacgaa ctcgggtttg acggcgtcga tgccggcgga atcgatgagt
     1861 cctggcgcca gcagcctgga accccggttt atggcgccga tctcgacgcc gccggcgtac
     1921 ggcgagccct ggcggaagcg aagagcgagc gcacaccgca gtggcgggcc acaaacaaca
     1981 gccccggcgc ctttggccgc cccgcctgac cccgtcttct cgcgaacggt gctagcattg
     2041 ttcccgtgac ccttcccggg ctgcttcatg cgtttctgat cggtggcgtc agcggtttac
     2101 gggcgatgac cgcgccggca atcgtcagtt ggggcgcgca tctgggatgg atcgaccttt
     2161 cgcagacgcc attggcgttt ctcggttatg ctgcgacacc ttacattatc agcctgctgg
     2221 ccattcttga actgatcgcg gacaagctac cgtcgacacc cagccgcaag gctcctccgg
     2281 gattcattgc gcgcctcggg ctcggcacat tttccgggta tgcgttgtcg ctgggcattg
     2341 gacaatccgg cgtcatcggc gctgtattgg gattcgcagg cgcaatcgca ggcacactgg
     2401 gcggctatga ggcacgaacc cgttcagtca aggcgttgaa agtaccggat atcgtggtcg
     2461 ccttgcttga agatgcaatc gccatcgcca tcgggcttgc cgccgttcat cacaaccagc
     2521 ctctgtgacg tatgaagagc gcttgttaga ctgaacagtg taaggaacag agtggtggaa
     2581 tccctcgtct tcctatgcga atgaagtcca gcgcgtcctt tatggaagcg gtgtttgttg
     2641 cggccgcttc gctccgctcc agcaaattac gtagttttct taccttactc ggtatcatcc
     2701 tcgccaccac cacgctgatc gccgtgatgg gaatcatcca cggcatggac gtctacatcg
     2761 ccacgcaggt atccgacatg ggagtggacg gctaccgtgt ccgccgggtc gtaatgatgg
     2821 gcgagcggga cccgaaaaga tggttgcagc tcaatcagcg aaacccgcaa cttacgcccg
     2881 aagaattcga gttccttcgc gagcgggtca cgctctcgcg tgaaattggg atggacgcct
     2941 cccgtggtgt cacggtgacc agtaagggtg tcgagattga tggcgcgcag ttgcagggtg
     3001 tgtccccaag cgtgggggtc atctccaacg tgccggtgga gacgggccgg tttctcagcg
     3061 acagcgacaa ccggagacgg ctctccgttg ccgtaatcgg gagcgatgtc gcagagaaat
     3121 tctttgccgg cgtagatgcc atcggcaaga ccatcgatgt ggaaggcagg ccgttcgaaa
     3181 ttatcggaac ctgcaaatcc cggggcagtg ttttcgggca gtcccaggat aacttcgtca
     3241 tgatccccgt tgagacgtac ttcaagatgt acggttcacg ctccggcgtg agcttcagcg
     3301 cgttggccct ggatcgcaat cacctcgaac aggcacagga cgaagtgcgg gcgctgctgc
     3361 gggcatttcg tcatctccgg ccgggagagg aagacaattt cggcatcatg gcctccagtt
     3421 ctctcgtcga catgtgggat cgcatgacgg gagccatcgc ggccacggcg gtgggtgttg
     3481 tttcggtgtt catggtggtt ggaggggttg tgatcatgaa catcatgctg gccgtggtca
     3541 ccgagcgaac gcacgaaatc ggcattcgaa agtccgtcgg cgccaggcgc cgggatattt
     3601 tgaaccagtt cctggtggag tcttccatgc tgtcggcggc gggcgggttc atcggtgtga
     3661 tggtggcctg gttcagcgcg atcgctgtcc gccggctcac acccgttc
//
LOCUS       DASTMK010000245         9407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_12728, whole genome shotgun sequence.
ACCESSION   DASTMK010000245 DASTMK010000000
VERSION     DASTMK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12728"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFQ79_10615"
     CDS             complement(<1..619)
                     /locus_tag="VFQ79_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140157.1"
                     /translation="MKTIVTSQLPVLALEIDQEGTVAQKSMQLMRQGQLDNTLLDALY
                     PRIRVATVEIPGSELDDYGRAKVEQALAKFTVGGVEFRLIGASGSAKNGKYYAVSTEF
                     EKPIAERFQQWPEAAITYFGILISPCKVRVEEPDVRVLVVEDHTLGTNDCRGWIRRSL
                     FEKLGLPARHFYQFRLAFNRTQAKGSFKVMENDVADHISADIILPK"
     gene            complement(766..1005)
                     /locus_tag="VFQ79_10620"
     CDS             complement(766..1005)
                     /locus_tag="VFQ79_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140158.1"
                     /translation="MENEYRALVKAARLLGDARAAVGREDGFGLISPAWKMLNHAQTH
                     LVQQADALLRGSDSCEQTSQLQEREAEVKEKTMTA"
     gene            complement(1150..1362)
                     /locus_tag="VFQ79_10625"
     CDS             complement(1150..1362)
                     /locus_tag="VFQ79_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140159.1"
                     /translation="MLKNKMATLLTVVSAMLISASMTWQQRLLFVGGIAFGVVVEALP
                     GLVDELKERVAEWRRIRQFTVKAKAA"
     gene            complement(1560..1877)
                     /locus_tag="VFQ79_10630"
     CDS             complement(1560..1877)
                     /locus_tag="VFQ79_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140160.1"
                     /translation="MGAATEPTTSGLAEPKAPAGKKERSSRRRQRTKTAEPSTARYFL
                     IKASGSDGLQLGEEVASENEALTASFRASGSYAVVTEWKANVDVSTGAPVIKKEGVQH
                     EKK"
     gene            complement(2043..2696)
                     /locus_tag="VFQ79_10635"
     CDS             complement(2043..2696)
                     /locus_tag="VFQ79_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140161.1"
                     /translation="MAGLFGVIALVSLGFAIYVRLQPATVIRISADGEATVVGAAPTN
                     TASETRSNLLSVLAATPAGEAAPTDIEGRAVVRKFLESYMRYTPTSVDKNLADALNMM
                     TANLRMYSLNLLRDQDLIGKIKEEQITSSFKIRSIEPVPGQAWTFQAFGVKEVHRVRN
                     KSEFTDRIVAKYHVRLIQERRTERVPSGLLVAEYREEQMVGEKDTGLLQESSLLGNR"
     gene            complement(2865..3719)
                     /locus_tag="VFQ79_10640"
     CDS             complement(2865..3719)
                     /locus_tag="VFQ79_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140162.1"
                     /translation="MFFFEQTFQTVLNGIDAPGGPMGAITNIANAILLLCALFAVYEA
                     YARGGDARMIGVAAAKFLLLGLIVSNYSTIFRNVNGAFNQVAATISPNDWADNWMLQV
                     NQYFNGLGNANWFNLVVSSIVALVSVLVQLIAAIVFPIALLIFTILYCLYGAVLYAVG
                     PMVLALYPAFGVGQLARSYLVNLFVFHAWGIVYALFSVLLTAINAQSLGAMLAANSLG
                     GWFQGASGALLMSLASILFAVMVALIPFLARRIVTGEIGSSMMTVVHTVGTALGAFKF
                     KPGRGDGN"
     gene            complement(3730..4560)
                     /locus_tag="VFQ79_10645"
     CDS             complement(3730..4560)
                     /locus_tag="VFQ79_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140163.1"
                     /translation="MPQILRFMRGLTSWVWTQRTKILVLVLMMTMVVPQPAKGQFIDV
                     AAIVSAIGAINTAITNVIGAGLRDISGALNAVNGVLNAIQNFFQNTVYPLDAINRARA
                     VVGAVQGFYNVIRGLVNLNVASATLANPRQLEAVLLSRNAGSIAQVANRFTTVYQAIP
                     PATDAPPPVRDLIDMTDATAQAALKKAIAIDAAADQLMDASDQLAAELAVAAPGTAPM
                     IEAQAAAMLVKAHALTQSATAELFRIRAIDMANTGASLKFNANHASDTRRDVTNMFKK
                     "
     gene            complement(4574..5416)
                     /locus_tag="VFQ79_10650"
     CDS             complement(4574..5416)
                     /locus_tag="VFQ79_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140164.1"
                     /translation="MKLLSLMFTSLLAFFLVTRVRRLVAVTLVGAIAFAVVAPPAKAQ
                     LGIPAIIAAAAQVVSTINNVIRPLLSAIQGTIGAINGVLNQFRSLWEQVVYPLQLINR
                     ARALVNSLIGQFRGLLPALMRVNVSSAQLPNPVLLESIIRNRGTGDFAQLTNAFGQTY
                     RAIPQPADAHPIERDLADIDDAMALANLKTLKASDAMVDQMMGAADAIEDEGVNMAPG
                     SAPFLAAAGIIASVKSQAMMQRMIAAATRQEAARVAHDNTVRKRNAMFGAQFRNDVQT
                     IFKK"
     gene            complement(5428..8085)
                     /locus_tag="VFQ79_10655"
     CDS             complement(5428..8085)
                     /locus_tag="VFQ79_10655"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140165.1"
                     /translation="MPTTIEQHNASLHQPAFSELLPVRDILHDVIIRTTGALVAGYEL
                     SGINSYYHSDDGRNRTKLALEALIRSLPERSMRMQVRFEIAEGLGDLRERYQQQLRNQ
                     NPTLLALDRVRMDVWRTKEREGYYLLPLLHAYFQWNPTVHHELPGAAFGKAMTKRFSL
                     DTFSLSANKCIQRTAREHEDVLAEFGSILSGVEQTLRATGMGVRRLTDAEIFLEMKRA
                     LNPLMEDRVPLRRPESSLQHRSAREQIVNTNIEHEEETYIRIGGLLYSFITLKDLPDA
                     TFPGILRDLVGLDFPITVNTDVTIPDQAAMIKHYKGRLKKMQAAQRDSHGGYRIDIDA
                     QVAQRQLIETLENLIASSLKTCRTSMVIGVRTSQPVQSHRDLAEQERVLSDRRQKVLY
                     TVMQMNGSRGLPEDLAKRRLFIGGLPGMSEENQRENDCLTVHAADLLPMETSWRGTPQ
                     SPLILLDTPQRQLVPFSPWDSSLADANLLIMAASGGGKTFMAMLFLLMMARLKPLISI
                     LERGDSYRPLVELMGGRSIEVDLEGSVTLNPWDLAPGATTPGKDKIAFLKNLTRHMIG
                     DSPNSDTALLDNLLSEAISRTYKRCAIRHSNPTPTFNDLREELQQWRDEERIERTVEE
                     AKLAALKLREWTGEKGVYSKLFDQHTTLKTDADWLFFNVERLSDDPKLETAMSMMIAH
                     AMQERASGRTGQHSITVLDECWSLLDSNVLAPEVVQLFRTARKRGGSVWGISQTLEDF
                     VGTDTQPRIHGPGIVRNVSTKIIGRQPGDVSPLAIHLALNQVALDEIKRFGAPRKGRS
                     AEALLVIGEKAETTQTIRLVPTPIEYWVCTTFPRERAYRTYCFANNPARIPLDVYQEL
                     AERFPNGLADVPALPEELSGAVAGAAAQR"
     gene            complement(8060..8422)
                     /locus_tag="VFQ79_10660"
     CDS             complement(8060..8422)
                     /locus_tag="VFQ79_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140166.1"
                     /translation="MALNISPVHRNLHTRVSFLYLEFEDWFVVIGLAALTNIFCRWID
                     RHLFGIPMNVFLQYIVPVLSIPFLMLFKYGKPRGYLKDFLAWHTKPRIYCGLEPDSGM
                     KLDYFKEEKIDANDYRTA"
     gene            complement(8441..8896)
                     /locus_tag="VFQ79_10665"
     CDS             complement(8441..8896)
                     /locus_tag="VFQ79_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140167.1"
                     /translation="MNFLTGITNLTNWTGNVIMPTLAALFFALGVLRYAHGQAHTYAP
                     WAGFLCLMVSGVLRGIEKFAAQAAWNNPDLVWITLRGLVNWTCNVFLPVYAVLQLLHG
                     FMMYAGIGHRIYAGGAWMRHFSAAALCLALSGLLRLAEWFIAQGLGGIS"
     gene            complement(8911..>9407)
                     /locus_tag="VFQ79_10670"
     CDS             complement(8911..>9407)
                     /locus_tag="VFQ79_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140168.1"
                     /translation="VMITLPQVFGWFAFWGVGAPLPGGGIGNGWMSTFANDAVIFVNS
                     FVIGRLVPVLAAWMVLRSVIDWSEGYTPLASILGAMFLLAIPASAALLQGWNDGTRFG
                     TAAVLEGAWTYTASRIMPIAAGLAVIGAVVNFAFGRPAIRLIGSAAAFLTVSALWRLV
                     VSMM"
BASE COUNT         1869 a         2915 c         2614 g         2009 t
ORIGIN      
        1 tttttggcag gatgatgtcg gcgctgatat gatcagcgac gtcattctcc atgaccttga
       61 agctcccttt ggcctgggtc cggttgaatg cgagacggaa ctggtagaag tgacgcgcgg
      121 gaagacccag cttttcaaac agagaacgcc gaatccagcc gcgacagtca ttcgtaccga
      181 gggtgtgatc ttctacgacg agcacccgca catccggttc ttcgacccgc accttgcagg
      241 gcgagatcaa gatcccgaag taggtgatcg cagcttccgg ccactgctgg aaccgctcgg
      301 cgatcggctt ctcgaactcc gtgctcacgg cgtagtattt gccgttcttc gcggagcccg
      361 atgccccgat gaggcgaaac tccaccccac ccacagtgaa cttcgccaga gcctgttcca
      421 ccttggcacg cccatagtca tccagctcgc tgccaggaat ctcaaccgtc gccactcgaa
      481 ttctcggata gagggcatca agcaacgtgt tgtcgagttg tccctgcctc atgagctgca
      541 tgcttttctg agctactgtt ccttcctgat cgatctccaa cgccaaaact ggcaattggg
      601 acgtaactat ggttttcatt gggcatctcc ttatctgttt ctgttgaatg agcaacagac
      661 agacaggagc gccgtctccg gtgctctccc tccgcctgtt gccgtgaact tgaatcaagt
      721 tcccgggtgc caggctcgaa gagccgtcac cgggttatcg tgctgctagg ccgtcatggt
      781 cttttccttc acctcagcct cacgttcttg taactgcgag gtctgctcgc acgagtcgct
      841 gccgcgcaac aaggcgtccg cctgctgcac cagatgcgtt tgagcgtgat tgagcatttt
      901 ccacgccggg cttatcaatc caaacccgtc ttcacggcca acggcggctc gggcatcacc
      961 aagcagtcgg gccgctttca caagcgctct gtactcgttt tccattgctc cttctccctt
     1021 ttcttcctgt cccacgagca acaggcagat cggagcgccg tttccggtgc ctctctgaag
     1081 cctgttgccg tgaactggat ccagttctcg gtgccaggct cgaaaagccg tcaccgggtt
     1141 gttgccctac tacgccgcct tcgcctttac ggtgaactgt cggattcgcc gccattccgc
     1201 gacacgttcc ttgagctcat ccacaagacc agggagcgct tccaccacca caccgaacgc
     1261 tatgccgcca acgaagagca gacgctgttg ccaggtcatg cttgcgctga tgagcatcgc
     1321 cgaaacgact gtgagtagtg ttgccatttt gtttttcaac atggatactc tcctcttccg
     1381 tctgttggca gaacagcaag gagcccgctg gaatgggtct cttcgctatc cgtggccagg
     1441 gttggtgaga actctggcca ggattcgcgt ggaaacccga ccagcacttt cctgtatcag
     1501 agctacaggt cgcttacaca cctacgtgca tgaagccagt gctgcccgtt atgaatgcgc
     1561 tatttcttct cgtgctgaac tccctccttc ttgatcactg gtgcgcccgt gctcacatcg
     1621 acgttggcct tccactccgt caccacggcg tagctgccgc tcgcacggaa cgatgcggtg
     1681 agcgcttcgt tttccgacgc gacctcctca ccgagctgga gcccgtcgct gcccgacgcc
     1741 ttgatcagaa agtagcgggc cgtcgaaggc tccgccgttt tggttcgttg ccggcgccgg
     1801 gaactgcgct ccttcttgcc tgccggagcc ttcggctcag cgaggccgct tgttgttggt
     1861 tccgtcgctg ctcccatcga atcgcctcct tccaactcat tcgcggcccg cgcccttgcg
     1921 agctgcgggc cgcgtcgcgt cctctctcag gtacgctgtt tcgccagatt gcgctggctt
     1981 cgctcccacc agcccccgat ccggagatgc cctctcgctg gaaccggggg cggcagaatc
     2041 catcaccggt tccccaggag gctcgactcc tgcaaaagtc cggtatcttt ttcaccgacc
     2101 atctgttctt ccctgtactc ggcgaccagc aggccgctcg gcacacgttc tgttcgccgt
     2161 tcctgaatca gccggacgtg atacttggct acgatccgat ccgtgaactc gctcttattc
     2221 ctgactcgat gaacttcctt caccccaaaa gcctggaacg tccacgcctg accagggacc
     2281 ggctcgatgg atcggatctt gaacgaggag gtgatctgtt cctctttgat cttgcctatc
     2341 aagtcctgat cccgcaggag attgagactg tacatccgca agttggcggt catcatgttg
     2401 agcgcgtcgg ccagattctt gtcgaccgac gtcggggtgt accgcatgta actctcgagg
     2461 aatttccgga ctacggcgcg gccttcaatg tcagtcggcg ccgcttcacc cgccggtgtt
     2521 gcggcgagca cactcagcaa gttgcttctc gtctccgatg cggtgttcgt cggcgcggcg
     2581 ccgaccacgg tcgcttcgcc gtccgcggag atgcgaataa cagtcgctgg ctgcagcctc
     2641 acgtagatgg caaacccgag ggagaccaaa gcgatcaccc cgaacagccc agccatcaac
     2701 atcgaccgat tcgcgtacgc tcgaagctgg ccatcatgct cgtaggactt cgtgtaccga
     2761 gccttctctg ggacttcttg gttgccgtca atctgtcgtt ttcttgagag catatgatct
     2821 tcacacctcc atgaaccggc cgctcttccc gaggcctagg ttccctagtt gccgtcaccc
     2881 cgccccggct tgaacttgaa ggctcctaat gctgtgccaa ccgtgtgaac taccgtcatc
     2941 atcgagctgc cgatttcccc ggtcacaatg cgccgcgcga gaaatggaat cagcgccacc
     3001 atcaccgcga acagaatgct cgccagcgac atcagcagcg ccccgctcgc accttggaac
     3061 cacccaccca ggctgttggc ggccagcatg gcgcctagac tttgagcatt gatggccgtg
     3121 aggagcacgc tgaataaggc atagacaatg ccccaggcgt gaaacacgaa caggttcacc
     3181 aaataactgc gcgccagttg gccaacacca aacgctgggt aaagtgcgag caccatcggc
     3241 ccgaccgcgt acaatacggc cccgtataag cagtagagta tggtgaaaat cagcagcgcg
     3301 atcggaaaga cgatagcggc gatcaactgc acgagcacgc tcaccagagc cacgatgctg
     3361 ctgacaacca gattgaacca attggcattc cccaggccgt taaagtattg attgacctga
     3421 agcatccagt tgtcggccca atcattggga gagatcgtcg ccgcgacctg attgaacgcg
     3481 ccgttgacgt tccggaagat cgtactatag ttggaaacga tcaaacccaa aaggagaaac
     3541 ttcgccgccg cgacaccgat catgcgcgcg tctccccctc gggcgtatgc ctcatagacg
     3601 gcaaagagcg cacaaagcag tagaattgca ttggctatgt tcgtaatcgc ccccattggc
     3661 cccccggggg catcaatgcc gttcaacaca gtttggaatg tctgctcaaa aaagaacatc
     3721 gcttcccctt tacttcttga acatgttcgt tacatctcgc cgcgtatcgc tcgcgtgatt
     3781 cgcgttgaac ttcaggctcg ccccggtgtt cgccatgtcg atcgctctga tgcggaacag
     3841 ttccgctgtc gcagactggg tcagcgcgtg ggccttcacc aacatggccg ccgcttgtgc
     3901 ctcaatcatc ggagccgttc ccggcgcggc cacggcgagt tcagcggcca gttggtcgga
     3961 tgcatccatc aactggtctg cggcggcgtc gatggcgatc gccttcttca aggctgcttg
     4021 cgcggttgcg tccgtcatat cgatcagatc ccgcaccggc ggcggagcat cagtcgccgg
     4081 aggtatggcc tgataaacgg tagtgaaccg gttggccacc tgcgcgatgc ttcccgcatt
     4141 tcgcgacagg agtactgctt cgagctggcg gggattcgcg agcgtcgcgc tggccacgtt
     4201 cagattcacc agcccccgga tgacgttgta gaaaccctgc acggcaccca ccacggcgcg
     4261 cgcgcggtta atggcgtcca gcgggtagac ggtgttctgg aagaagttct gaatggcgtt
     4321 tagcaccccg ttcaccgcat tcagcgcacc actaatgtcg cgcaagccgg cgccgatgac
     4381 gttcgtgatg gcggtattga tcgcccctat cgccgacacg atcgcagcga catcgataaa
     4441 ttgacccttt gcgggctgcg gcacgaccat tgtcatcatc aggaccagca ccagaatctt
     4501 ggtgcgttgt gtccacaccc aactcgtcag gcctctcata aatcgaagaa tctgcggcat
     4561 ggagtttctc tccttacttc ttgaagatcg tctgcacgtc gttccgaaac tgtgctccaa
     4621 acatcgcatt ccgcttccgc acggtattgt cgtgcgccac acgcgccgcc tcctggcggg
     4681 tggccgccgc gatcattcgc tgcatcatcg cctggctctt cacagaggcg atgatccccg
     4741 ccgccgctag gaacggcgct gagccaggag ccatattcac tccctcgtct tcaatcgcgt
     4801 cagcggctcc catcatctgg tccaccatcg cgtccgacgc cttgagcgtc ttgagattcg
     4861 ccagggccat agcatcgtcg atgtccgcaa gatcccgttc tatggggtgc gcgtccgctg
     4921 gttgaggaat tgcacggtag gtctgaccaa aggcgttggt cagttgagcg aagtctcccg
     4981 taccgcggtt gcgaatgata ctctcgagca agactgggtt cggcagttga gcgcttgaca
     5041 cattgacccg catcagagcg gggagaagac cgcgaaactg cccgatcaat gaattgacca
     5101 gcgctcgcgc tcgattgatc aactgaagtg ggtacacgac ctgttcccag agactacgga
     5161 actgattgag cactccgttg attgccccaa tcgtgccctg aatggcgctt agcagaggcc
     5221 tgatgacgtt attgatcgtg ctcactacct gcgctgccgc tgcgatgatg gccgggatgc
     5281 cgagttgagc ctttgccggc ggcgcgacga ccgcaaacgc aatcgctccg accaaggtca
     5341 cagccacaag ccggcgcact cgggtcacca ggaagaacgc cagcaggctt gtaaacatga
     5401 gcgataggag cttcataaag tgaatcctta cctctgcgcg gccgcaccag ccactgcacc
     5461 cgatagctct tcgggaagcg ccggcacgtc cgcgagtccg ttcggaaacc tttcagcgag
     5521 ttcctggtaa acgtccagcg gaatccgagc cggattattg gcgaagcaat aggttcgata
     5581 agcgcgttct cgcgggaacg ttgtacacac ccaatactcg atgggcgttg gcacgagacg
     5641 aatcgtttgc gtcgtttcgg ccttctcacc gatcaccagt agcgcctcgg cactccggcc
     5701 ttttctcggc gcgccgaagc gcttgatctc gtccaacgcc acttgattca gtgccagatg
     5761 gatcgcgagc gggctgacat ctcccggctg gcggccaatg atcttcgtcg aaacattgcg
     5821 cacgatgccg ggaccgtgga tgcgtggctg cgtgtccgtc ccgacgaagt cctcaagcgt
     5881 ttgcgaaatg ccccagacgc taccgccgcg tttgcgcgct gtgcggaata gctgcacgac
     5941 ttcgggagcc aggacgttgg agtcgagcaa cgaccagcac tcgtcgagga cggtaatgct
     6001 gtgttggccc gtcctgccgg aggcccgctc ctgcattgcg tgggcaatca tcatgctcat
     6061 cgcggtttcg agctttgggt cgtcgctcag cctttccacg ttgaagaaca gccagtcggc
     6121 gtccgtcttc agcgtggtgt gctggtcgaa caatttcgag taaacgccct tttcgccagt
     6181 ccactcgcgg agtttgagag ccgccaactt ggcttcctcc acggtccgct cgatgcgctc
     6241 ttcatcacgc cattgttgga gttcttctcg cagatcgttg aaagtcggcg tcgggttgct
     6301 gtgccggatg gcgcagcgct tataggtgcg gctaatcgcc tcactgagca ggttgtcgag
     6361 cagggctgtg tccgaattcg ggctgtcccc gatcatatgg cgggtaagat ttttaaggaa
     6421 ggcgatctta tcctttccgg gcgtcgtcgc acccggcgcc aggtcccagg gattgagagt
     6481 tacgcttcct tcgagatcca cctcgatcga ccgcccaccc atcaactcca ccagcggacg
     6541 gtacgagtct ccacgctcaa gaatcgagat cagcggcttc agtcgtgcca tcatcagcag
     6601 gaacagcatc gccatgaagg ttttgccgcc gcctgacgct gccatgatca gcaggttcgc
     6661 gtccgcgagc gatgaatccc acggagagaa cggcacgagt tgacgctgag gcgtatcgag
     6721 cagaatcagc ggcgactgcg gcgtgccgcg ccaggaagtt tccattggca gcaggtctgc
     6781 cgcatgcact gtcaggcagt cattctcccg ctgattttct tcgctcatac cgggaagacc
     6841 cccgatgaac aaccgccgct ttgccaagtc ctcgggaagc ccacggcttc cgttcatctg
     6901 catgaccgtg tacagaacct tctgacgccg gtccgacaag acccgctcct gttcggcgag
     6961 gtcacggtgg ctctgcacgg gttgcgacgt gcggacaccg atcaccatgc tggtgcgaca
     7021 cgttttgagc gaactcgcaa tcagattctc gagtgtctcg atcaactggc gctgcgccac
     7081 ctgcgcgtcg atgtcgatgc ggtacccgcc atgcgagtcc cgttgggcgg cctgcatttt
     7141 cttgaggcgc cccttgtagt gtttgatcat cgccgcctgg tccggaatag tgacgtcggt
     7201 gttgaccgtg atggggaagt cgaggccaac aagatcgcgt agtatccccg ggaacgtggc
     7261 atcgggcagg tccttcagag tgatgaaaga gtacaggagg ccgccgattc gaatgtaggt
     7321 ctcttcctca tgttcgatat tcgtattgac gatctgctcc cgtgcgctac gatgctgtag
     7381 tgaagactcc ggccggcgaa gcggtactcg gtcttccatc agcggattca gggcgcgctt
     7441 catctcgaga aagatctctg cgtctgtcag ccgtcggact cccatccccg tcgctctcaa
     7501 cgtctgctcg acacccgaga ggatggaccc gaattcggcc aagacatctt cgtgctcccg
     7561 cgccgttcgt tggatacatt tattcgcgga caggctaaag gtgtcgaggc tgaagcgttt
     7621 cgtcattgcc ttgccgaaag ccgcgccggg aagttcgtgg tggactgttg gattccactg
     7681 gaagtacgca tgcagaagcg gcagcagata gtagccttct cgctccttcg ttctccagac
     7741 gtccatcctg acgcgatcga gggccaagag cgttggattc tgattccgga gttgctgctg
     7801 atatcgttcc cgcagatcgc cgagcccttc ggcaatctcg aaccgcacct gcattcgcat
     7861 ggatcgctcg ggcagtgacc ggatcagcgc ttcgagcgcc agcttcgtcc tgttcctgcc
     7921 atcgtcgctg tggtaatacg agttgatgcc actgagttca tagccggcga ctaatgcgcc
     7981 cgtggttctg atgatcacgt cgtgcaaaat gtcgcgcacc ggcagcaact cggagaatgc
     8041 tggctggtgc agagacgcgt tatgctgttc gatagtcgtt ggcatcgatc ttctcctctt
     8101 tgaaatagtc gagcttcatt cccgagtcgg gctcaagccc gcaatagatc cggggcttag
     8161 tgtgccaagc cagaaaatcc ttcaggtagc ctctaggttt tccgtatttg aacagcatga
     8221 gaaatgggat gctcagcact ggaacgatat actgcaagaa cacgttcatc gggattccga
     8281 ataggtggcg atcaatccaa cggcagaaga tgttggtgag ggctgccagg ccgatcacga
     8341 cgaaccaatc ttcgaactcc aggtagagga agctcactcg cgtgtgcaga ttgcggtgaa
     8401 ccgggctgat gttcaatgcc attgccttct tatcccccta tcagctaatg ccgcccagtc
     8461 cttgcgcgat gaaccattcc gccagccgca gcaagccgga caacgccaag cacagtgcag
     8521 cggcgctgaa atgccgcatc caggcaccgc cggcataaat gcgatgaccg atgcccgcgt
     8581 acatcatgaa tccgtgcagc agttgcagca ccgcgtacac cgggagaaag acattgcacg
     8641 tccaattcac caggccccga agcgtgatcc aaaccaggtc cgggttgttc cacgcagctt
     8701 gcgccgcgaa tttctcgatg ccacgcaaga cacccgagac catcaagcag agaaagcccg
     8761 cccacggtgc ataggtgtgc gcctgcccgt gcgcgtagcg gaggacgccg agcgcgaaga
     8821 acaacgctgc gagcgtcggc atgatgacgt tgcccgtcca attcgtcagg tttgtaatgc
     8881 cagtgaggaa gttcatgcgg cctcaagttg ctacatcatc gaaacgacca agcgccacag
     8941 agcggagacg gtcaggaacg ctgcggcgga accgatgagc cggatcgctg gccggccgaa
     9001 cgcgaagttc acaacggcgc caatgacagc caggccggcc gcgatcggca tgatccggct
     9061 ggcggtgtac gtccaagccc cttcgaggac cgcggccgtc ccaaaccgcg tcccgtcatt
     9121 ccaaccttgg agaagagcag cgctcgcggg gatcgccagt aggaacatgg cgccaaggat
     9181 cgaggctaga ggagtgtagc cttcgctcca gtcgatcacg gaccgaagaa ccatccacgc
     9241 cgcgaggacc ggcactaaac ggccgatcac gaagctgttc acgaagatca cggcgtcatt
     9301 cgcgaacgtc gacatccatc cgttgccgat gcctccacca ggaagtggcg caccaacacc
     9361 ccaaaaggcg aaccatccga atacttgcgg gagggtgatc atcacga
//
LOCUS       DASTMK010000246         6278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_492098, whole genome shotgun sequence.
ACCESSION   DASTMK010000246 DASTMK010000000
VERSION     DASTMK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492098"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFQ79_10675"
     CDS             <1..589
                     /locus_tag="VFQ79_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0140169.1"
                     /translation="IEDTSLACSLPGFVVLSPADEVTMRALVRAAVQHVGPVFIRAGR
                     PKVPILYSPDQQFEIGKAITLTEGTDVTLLATGLMVVESIKAQEILENEGISARVIDI
                     HTIKPLDVGLIDKAASETGAIVVSEEHLVDGGLGVRVAQAVAQTSPCVMEFVGLTDYA
                     ESGSPEDLLRKYGLVAPDIAEAARKAVARKKKNKS"
     gene            603..1280
                     /locus_tag="VFQ79_10680"
     CDS             603..1280
                     /locus_tag="VFQ79_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0140170.1"
                     /translation="MSDPSTSLAKSLVTLLRAREGEYERVSRLLHDEIGQVLSAVGLQ
                     LDVLRLDYKDQLPEIAARTAEIQNLLERAMKQVRDLSYLLNPAIVERAGLHSALDRLV
                     GRSRALFPGPIRLLLDSSVRIPPDVGTAFYRIAEQAIENAVQHSGATLIEVLLKPARN
                     AASLEIRDNGTGFSPDAHDGENGLGLALMHYYAEQKNLEFKIRTGADEGTIISVLYVP
                     NELTQRL"
     gene            1312..1998
                     /locus_tag="VFQ79_10685"
     CDS             1312..1998
                     /locus_tag="VFQ79_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0140171.1"
                     /translation="MPFEVLLVDDHKIMRDGIKAILKHAGEFTVVAEAEDGAEAVQVC
                     KRTGPDIVVMDIGLPGLNGIEATTEINRHCPGTRVIILSMYDDEHSVVSAIRSGARAF
                     VLKRASDSDLLDALRTVAKGGSYLSPQVSDRLLSRIQRGDLESKPASSALDGLSPREL
                     QVLRLVAEGKTSKDIAVMLDLGLQTVRSYRKTMMKKLGVNNVAGLTQLALAAGITRFG
                     ATEDSPVEAQ"
     gene            2002..2916
                     /locus_tag="VFQ79_10690"
     CDS             2002..2916
                     /locus_tag="VFQ79_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0140172.1"
                     /translation="MKTPWGDLPVADAHVHFFSHRFFQLLTASSPNPLPVESLEGLLG
                     WEMPPENPEQLAARWVGELDKHGVECASLIASAPGDEDSVIAAVQAFPGRFRGCFMVN
                     PCAPGAPDKTKRALEAGLRVICLFPAMHRYSIQSEPVERLLEPVSSQTGACVFVHCGV
                     LTVGVRKKLGLPSLFDMKYSNPIDLHAVALRFPALPFIVPHFGAGYFREALMLADLCP
                     NVYLDTSSGNSWTRYLGPGMDLAAVFRMALDVTGPQRLLFGTDSSFFPRGWNAEIFRA
                     QVQALESLPITAADAASILGGNFHRLLS"
     gene            2963..3769
                     /locus_tag="VFQ79_10695"
     CDS             2963..3769
                     /locus_tag="VFQ79_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140173.1"
                     /translation="MKFHKHWSREKSGFTPEQRLRLERVLRAAAETGFYGEQPLSLKA
                     FSTLPPADPYEVAARLSVFRGEALSSETGPLLHYPIHPLPRLALLGTSLKSDGDVREF
                     VGPWSPALQRFRPQALVAPGPVLLALTDSVQRRQVSLNSLTHSIVALSGPLYGILSAQ
                     DRDRLWRTWRVPLFEQFRGCREELLAWECEAHCGLHFRDEQLLIEAADGDSGLLITDL
                     TSLDYPVLRLASGIEGTVETAPCGCGRTLPRLTNLRTTEGFRVARAAAAV"
     gene            3836..4804
                     /locus_tag="VFQ79_10700"
     CDS             3836..4804
                     /locus_tag="VFQ79_10700"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0140174.1"
                     /translation="MAALSEIHAPELTELRRVRVDDLDAVLNEEECVWKDRLSWDFRP
                     SADLVRRFVGVQALSGFALVAGGRVIGYSYFVTEDQKGLVGDLYILRDFATAELEYQL
                     LGAVLDRMMSSPFLHRVESQLMMLHSPLEADLPHASFARCYPRNFMVVDLRASGKLPV
                     GKARQNIVLEPWSERFQEDAARVIASAYQGHIDSEINDQYRSVQGARRFLVNIVQYPG
                     CGSFFQPASFVAQQQGSGRTAGICLSSLVAPEVGHITQVCVMPGSKGSGIGYELVRSS
                     LLALAEHGCHSASLTVTSTNREAVKLYERMGFVTKRNFSAFVWEGF"
     gene            complement(4817..5437)
                     /locus_tag="VFQ79_10705"
     CDS             complement(4817..5437)
                     /locus_tag="VFQ79_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0140175.1"
                     /translation="MYRLYIALLTCAAAFAQVSHQHHPPRDTGEYARVLNNPSREKWQ
                     KPGEVVQALQIRPDEVIADIGAGTGYFARRFARHAAKVYAVDIEPRLLEHAAKDAPEN
                     LITVHAAPDDPKLPPNSVDTVFFCNVLHHIENRPEYLKKLAAALRPGGRIVNIDFYKK
                     ELPVGPPPSMKLTEEEVVSTFKAAGFQQSKSLDFLPYQYFLVFVKQ"
     gene            complement(5452..>6278)
                     /locus_tag="VFQ79_10710"
     CDS             complement(5452..>6278)
                     /locus_tag="VFQ79_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0140176.1"
                     /translation="VLAILIFVFGAARAVEPSNWRPYMPNGFGGVLTGAAIVFFTYIG
                     FDSVSTAAEECKKPQRDLPIGIIGTLVVCTLLYVSVAVVLTGITNYQTLANAAPVVNA
                     LKALGYDDIRRWVGVGAIIGMVSSLLVFQYGQARIWFAMSRDGLLPSLFSKVHPVHRT
                     PHVSTWVAGLAVGIPAGIWDVGTFADLSNIGTLFAFVLVSAGVIVLRRRQPERRRSFR
                     VPLVPWLPLFSIGCCLVLMLSLPLETWIRFAVWLAIGLAIYFLFGKKHSQFADRRN"
BASE COUNT         1256 a         1819 c         1909 g         1294 t
ORIGIN      
        1 tattgaagac acgagcctcg cctgctcgct cccggggttc gtcgtgctct cgccggcgga
       61 tgaagtgacg atgcgcgccc tggttcgtgc cgctgtccag catgtgggtc cggtgtttat
      121 ccgcgcggga cggccgaaag tccccatcct ctattcgccg gaccagcagt ttgagattgg
      181 caaggccatc acgctcacgg aaggaacgga tgtgacgctg ctcgctaccg ggttgatggt
      241 ggtcgaatcg atcaaggccc aggagattct ggaaaacgag ggcatatcgg ccagagtgat
      301 cgatattcac acgatcaagc cgctcgatgt cggcctgatt gacaaggccg cctcggaaac
      361 cggcgccatc gttgtttcgg aagagcatct ggtcgacggc ggcctcggcg tgcgagtcgc
      421 tcaggctgta gcccaaacca gcccctgtgt catggagttt gtcggcctga cggattacgc
      481 cgagtccggg agccccgaag atctgcttcg caaatatggc ttggtcgctc cggatatcgc
      541 cgaagcggcc cgcaaggcag ttgcccgaaa gaaaaagaat aagtcctgat ccgggacatg
      601 ccatgtctga tccctccacc tcgctcgcca agagcctggt tactcttttg cgagcacgcg
      661 aaggcgagta tgaacgcgtc tcgcggctgt tgcatgatga aatcggccaa gtcttgagcg
      721 cggtagggct ccaattggac gttttacggc tcgactacaa ggaccaactg ccggagatcg
      781 ccgcgcgaac cgcggagatc cagaacctgc tggaacgcgc gatgaaacag gtccgtgacc
      841 tcagctatct tctgaacccc gctattgtgg aacgcgcggg gcttcactcc gcgctggacc
      901 ggctggtggg gcgatcccgc gccttgtttc caggcccgat ccggcttctt ttggattcgt
      961 cggtccgcat tcccccggat gtgggtaccg ccttctatcg catcgccgaa caagccatcg
     1021 aaaatgccgt acagcattcc ggcgccactc tgattgaagt gttgctcaag cccgccagaa
     1081 acgccgcgag cctggagatt cgcgataatg gaaccgggtt ctcgcccgat gcccacgatg
     1141 gagaaaacgg gctgggacta gctctgatgc attactatgc cgagcagaag aatctcgagt
     1201 tcaagatcag gaccggcgca gatgagggta caatcataag cgtactgtac gtaccgaatg
     1261 aactcacgca acgcctttaa ggcactgaaa ggtgcgggat gagggaatga aatgcctttt
     1321 gaagttctcc tcgtagatga ccacaagatc atgcgggacg gtattaaggc catcctcaaa
     1381 cacgcaggcg agttcactgt cgttgccgaa gccgaggatg gggccgaggc ggttcaggtc
     1441 tgcaagcgca ccggtcccga tatcgtcgta atggacatcg ggcttcccgg gctcaatggt
     1501 attgaagcga ccacggaaat caaccggcat tgtcccggga ccagagtcat catcctttcg
     1561 atgtatgacg acgaacattc cgtggtcagc gctatccgct ccggagcgcg cgctttcgta
     1621 ttgaaacggg cctccgatag cgatctgctc gacgcgctcc gcacggtcgc aaaaggagga
     1681 tcgtatctta gccctcaggt atcggaccgt cttctaagtc gcatccagcg tggcgatctg
     1741 gaatcgaagc cggcctcttc cgctctcgac ggcctctcgc ccagagagtt gcaggtgctg
     1801 cgtctggtgg ccgaaggcaa aaccagcaag gacatcgccg tcatgcttga tctgggtctg
     1861 cagacggtgc ggagctatcg caagactatg atgaagaagc tgggcgtcaa caacgttgcc
     1921 gggcttacac agcttgcatt ggccgcgggc attacgcggt ttggcgcgac tgaggatagt
     1981 cctgtcgaag ctcagtgagc gatgaaaacc ccctggggcg acctccctgt cgccgacgct
     2041 cacgttcatt tcttctcgca tcgttttttc caattgctca cagcctcaag ccccaacccg
     2101 ctacctgttg aaagcctgga gggactgctc ggttgggaga tgccgccgga gaatccggaa
     2161 caactggccg cgcggtgggt gggggaactc gataaacatg gggtagaatg cgcttccctc
     2221 atcgcctcgg cgccgggaga tgaagactcg gtgatcgcgg cggttcaggc ctttccaggc
     2281 cgcttccggg gctgctttat ggtgaacccc tgcgcacccg gcgctcccga taagacaaaa
     2341 cgtgcgctcg aagcggggct acgcgtcatc tgcctgttcc cggccatgca tcgttattcg
     2401 atccagagcg aaccagtgga gcgtttgctg gaacccgtgt catcgcagac gggtgcctgc
     2461 gtatttgtgc actgcggtgt gctcaccgtg ggggtgcgga agaaactggg gctcccatcg
     2521 ctgttcgaca tgaagtactc gaacccgatc gatcttcacg cggtcgccct ccgcttcccc
     2581 gccctgccct tcatcgttcc gcacttcggc gcgggctatt tccgtgaggc gctcatgctg
     2641 gcggatctct gcccgaatgt ttatctcgat acgtcgagcg gcaatagctg gaccaggtat
     2701 ttaggcccgg ggatggatct tgcggccgtc tttcgcatgg ctctcgatgt tacaggcccc
     2761 cagcggctgc ttttcgggac agactcctcg ttttttccga gaggctggaa cgcggagata
     2821 ttccgggcgc aagttcaagc gctcgaatca cttccgatta cggcggcgga cgcagcatcc
     2881 attctcggtg ggaatttcca tcgtctttta agttaaacac ctgagatttc tgccagcctc
     2941 gcggcacgaa gccagatagg cggtgaaatt ccacaagcac tggtcgcggg agaaaagcgg
     3001 tttcactccg gaacaacgcc tgcgtctgga gcgcgttctg cgtgcggccg cagagacggg
     3061 tttttacggc gagcaaccgc tttccctcaa agcgttctct actctgccac cggccgatcc
     3121 ctatgaagta gcggccagac tttccgtatt ccgcggggaa gcgctgtcct ccgaaacggg
     3181 tcccctgctg cactacccga ttcatccgtt gccgcgcctg gccctgctgg gaaccagtct
     3241 gaaatcggac ggggatgtac gggagttcgt tggaccgtgg agtcccgctc tccagcgctt
     3301 ccggccgcag gcactcgtgg cgccgggacc tgttctgctt gcgctgacgg actccgttca
     3361 gcgccggcag gtttctctca attcgctgac gcactccatt gtagccctct cgggacctct
     3421 ttatggcatc ttgagcgcgc aggatcgcga ccggctctgg cgcacgtggc gagtgccctt
     3481 gttcgagcaa ttcaggggat gccgcgagga gttgttagcc tgggagtgcg aagcgcattg
     3541 tggcctgcat ttccgggacg agcaacttct catcgaagcg gcggacggag actcgggcct
     3601 gctgattaca gacctcacga gtttggatta cccggtgctt cgcctggcaa gcggcattga
     3661 agggaccgtc gagacggcgc cgtgcggttg cggacgcaca ctcccgcggc taacgaatct
     3721 caggaccaca gagggcttcc gggtggctcg cgctgccgcc gcggtctgat tccaaatcaa
     3781 accggagccg gacagaattt tccagatata aatccaatgg gcttataatc ctcctatggc
     3841 cgcactctcg gagatccacg ctccggaact gacagagttg aggcgggtta gggtggatga
     3901 ccttgacgcc gttctcaatg aggaagagtg cgtgtggaag gacaggcttt cctgggattt
     3961 ccgcccgtcc gccgatcttg ttcgccggtt cgtcggcgtg caggctctga gcgggtttgc
     4021 tctggtggcg ggcgggcgtg tgatcggcta ttcctacttc gttaccgaag accagaaagg
     4081 gctggtcggc gatctttaca ttttgcgcga cttcgctacg gcggaactgg agtatcaact
     4141 gctgggagcg gttctcgacc ggatgatgtc atcgcctttt ctgcatcgtg tggagtcgca
     4201 gctcatgatg cttcattccc cgcttgaagc ggacctgccg cacgcttcgt tcgcccggtg
     4261 ctacccgcgc aatttcatgg tggtcgatct ccgcgcctca ggaaaactcc cggtgggaaa
     4321 ggcgcggcag aacattgtgt tggaaccttg gtcggaacgg tttcaggaag atgccgcgcg
     4381 cgtgattgcg agtgcttatc aagggcacat cgatagtgag attaacgacc agtaccggtc
     4441 cgtgcagggc gcgcgccgct tcctggtaaa cattgtccaa tatccaggtt gcggcagctt
     4501 ttttcaaccc gcttcgttcg tggcccagca gcagggctca ggacgaacgg ccggcatttg
     4561 cctgagcagt cttgtcgcgc cggaagtagg acacatcaca caagtgtgcg tgatgcccgg
     4621 ttcgaagggc tcgggaatcg gatacgagct ggtgagaagc tcgcttctcg ctctagccga
     4681 gcatggatgc catagcgcca gccttaccgt aacgtcgacg aaccgcgagg ccgtaaagct
     4741 ttacgagcgg atgggctttg tgacgaaacg caacttctcc gcttttgtgt gggaagggtt
     4801 ctgagcggcg ccggcctcac tgtttcacga acacaagaaa gtactgatag ggcaggaagt
     4861 ccaggctctt gctctgttgg aaccccgcgg ctttgaaggt actgacgact tcctcttcgg
     4921 tgagcttcat cgacgggggc ggaccaaccg gaagctcctt cttataaaaa tcgatattca
     4981 cgatacggcc gccggggcgc agcgccgccg ccagtttttt caaatactcc gggcggttct
     5041 cgatatggtg cagaacgttg cagaagaaga ccgtatccac cgaattcggc ggcagcttcg
     5101 gatcgtccgg cgcggcgtgc actgtgatca ggttttccgg agcgtctttg gccgcgtgtt
     5161 cgagcagcct gggttctatg tccacggcat acacttttgc cgcatggcgg gcgaaccggc
     5221 gggcgaagta ccctgttccg gcgccaatat ccgcgatcac ttcgtcgggc cggatctgca
     5281 gggcctggac cacttcgccg ggcttctgcc atttttcgcg cgacggatta ttgagaacac
     5341 gggcatattc gccggtatcg cggggtggat ggtgctggtg agaaacctgg gcgaatgctg
     5401 cggcacaagt gaggagagcg atatagagcc ggtacataac aattatcgta ttcagtttct
     5461 ccggtccgcg aactggctgt gctttttgcc gaacaggaag tagatcgcaa ggccaatggc
     5521 caaccagacg gcgaaacgga tccatgtttc aagaggaaga ctcaacatca gcacgaggca
     5581 gcagccgata gagaacaggg gcaaccacgg tacaagagga acgcgaaagc tgcgccggcg
     5641 ctccggctgc cggcggcgca ggacgatgac tccggccgag accagcacga acgcgaacaa
     5701 agtgccgatg ttggacagat cggcaaacgt gcctacatcc cagatccccg cggggattcc
     5761 caccgcgagc cccgctaccc acgtgctgac gtgcggcgtg cgatgcacgg gatgtacttt
     5821 cgaaaacaag gaaggcagca gtccgtcgcg cgacatggca aaccagatac gcgcctgccc
     5881 gtactgaaaa acgagcagcg aggagaccat gccgatgatg gcgcccacgc cgacccagcg
     5941 gcggatatcg tcatacccca gcgctttgag tgcattcacc accggcgcgg cattggccag
     6001 cgtctgatag ttggttatgc ccgtcagaac cacggcgacc gagacataga gaagggtgca
     6061 gacgacgagc gtaccgatga tgccgatagg aagatcccgc tgcggctttt tgcattcctc
     6121 cgccgcggtg gatacggaat cgaagccgat gtaagtgaag aacacgatgg cggcgcccgt
     6181 cagcacaccg ccgaatccat tcggcatgta gggccgccag ttggagggct cgaccgcgcg
     6241 cgccgcgccg aacacgaaga tgagaatcgc caatactt
//
LOCUS       DASTMK010000247        12937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_587772, whole genome shotgun sequence.
ACCESSION   DASTMK010000247 DASTMK010000000
VERSION     DASTMK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587772"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFQ79_10715"
     CDS             <1..600
                     /locus_tag="VFQ79_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0140177.1"
                     /translation="RSAVAAAKAVSMPADNIKRAIMRGTGELEGGQIDEVTFEGYGPG
                     GAAVLVSVATDNRNRTVSEIRHVFSKNGGNLGEQGSVAWMFERKSQILIERDKANEDD
                     LMALVLDAGAEDLRDDGSNWEVLSAPEAHDAVFEAIQKAGIPTVSAEIAMIPKNLVKL
                     EGKNAAGMLRLSEALEEHDDVQSVYSNFDIDEKELEALA"
     gene            618..1130
                     /gene="ruvC"
                     /locus_tag="VFQ79_10720"
     CDS             618..1130
                     /gene="ruvC"
                     /locus_tag="VFQ79_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU0140178.1"
                     /translation="MRVLGIDCGSERTGYGVIETDGKTHELLAAGVIRTSAKEAPEKR
                     LLRIAMGLREVMEKFSPQAAAVEEVFHGVNARAALKLAQVRGVALLTAAEAGVPVGEY
                     SPLEIKTSVVGYGRAEKRQVQLMLQSLLHLDRRLDSEDASDAVAVAVCHATRSAALLR
                     TAFSGPGGEA"
     gene            1127..1759
                     /locus_tag="VFQ79_10725"
     CDS             1127..1759
                     /locus_tag="VFQ79_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140179.1"
                     /translation="MRYLVFLFAVVAALGADLPRITYSKSFPGSVPPYVEISVDKNGM
                     AVYKESADDESPLKFRLTEAEASEVFALAAKLDHFRRPLESGLKVAKMGKKSFRYEDG
                     NVKNEVHFNFSQDLDARTLTDWFERMTETEQQFINLQRTTRFDKLGVNQALIQLQAAH
                     ERKRLVAPEQFLPLLERIVKNDTYMHMSRERAAGLAEAFRASTAGKSGAE"
     gene            complement(2298..2561)
                     /locus_tag="VFQ79_10730"
     CDS             complement(2298..2561)
                     /locus_tag="VFQ79_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140180.1"
                     /translation="MSPVTSELRWLEVLCEAELREERVVPPKRLVADRKAPEEEVPLE
                     PAKPEPPDACPGEAVCGVPVPVEETVGVEGEDPLPVEVAVEAT"
     gene            2635..3660
                     /locus_tag="VFQ79_10735"
     CDS             2635..3660
                     /locus_tag="VFQ79_10735"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140181.1"
                     /translation="MQQLIKPGSHVKKGEMLAEFDRQYMLLRLDDYRVSVDQQGKELV
                     TLKANLDVAKKAHEQKIVAAKGDLDQAALDLKTLPVRSAIDSERLKLAHEEALARYKQ
                     LLSEVKHFERSQQAQYRIADLDFRQSQLELQRSEANTNRMVANAPINGLTVMQNTFRG
                     TEFGQIQEGDQLRPGQPYMQVVDVSAMVIEAKVSQVDVEHLRIGAKAVVRFDAYPDLV
                     LPAHVFSIGAMTNPGGMRASFVREVAVKLRLDELDPRVIPDLSVSADVVIETAPEPST
                     LIARESVFRDSADTDPYVFVRAGTAWVRRNVELGLANNISVAVTSGLEKGEVVALSRP
                     PAASKGQ"
     gene            3671..4975
                     /locus_tag="VFQ79_10740"
     CDS             3671..4975
                     /locus_tag="VFQ79_10740"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140182.1"
                     /translation="MSKDLKPKHRLGQVESSRKKKIIVIAVLVALAVTGGYFAYRRTN
                     TTEVEVPVARVRTGEFIIAVRARGEIRSTRSMVLTAPQVPNPQIVRLAESGKPIRKGE
                     VVVEFDAAAQEQALLERSTSVRTADSEIVQTRAAHRITDEMDAMNLMTAGYNLERAKL
                     EASKAEILSEIEGAKNRIEVGISEGELGQVKTTIGAHKTTQKADLERLQQRKDKAVRD
                     MDRAKGYLSRMVIRAPNDGVVNILPNFRAPGSFGSSPPPFKEGDRAWTGAAIAEIPDL
                     SEMRIELKLDEVDRGKIALGQPLRIRVDAIPDKEFSANLDWISPIAALNFRGFGLTEK
                     LFPARATLKNLDSRLRPGMSASADIIIERQSKQLLIPARASFMHAGKPAVYVQRGKDF
                     QIRQIEVGKRNDSDIIVLNGLKEGEIIALENPIEAAKKAKKL"
     gene            4980..6899
                     /locus_tag="VFQ79_10745"
     CDS             4980..6899
                     /locus_tag="VFQ79_10745"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140183.1"
                     /translation="MPARRYSFKKILIRTGIFAVVLAACWGGYVLVQQMPQRQQQVAT
                     TKVRKGDVVVRSYARGELQAVRSASLVAPNLFGTVQVTRLAYLGSFAREGDLIVEFDD
                     SEVRARLEEKQLELDQVDEQIRKAEADLAIRNNQDQVELLNARYSVRRAELEVKRNEL
                     LSEIDRKKNLLTQDEAQRRLKQLESDIKSRLEQSKAEMAVLFERKNKAVLEMARERQR
                     LGLVKLLAPMSGLVAIRQNFSGGFRMFGMQIPDVREGDQVSPGMLVADVLDLSELEIS
                     AKVGELDRANLREGQDVLVQLDAIPEKQFTGRIKSMSGTASANFFSADPAKKFDVTFS
                     IDMKTLMSALGAKSEQIARVLATAAKNRGKPSTPAPVPMAGMAAPGVPPPGAMAGGGM
                     VGSSMGGGPGVVGGDVAPGAAGAQGGRRAFAGGGGPSMSDGDRQKMREAMEKALKGRS
                     IQDLSPEERQKVFAEARKAAGLGAGSPGGRQRRDGEAGPGGPSAPMPRLPAMVYGGSS
                     IFTQVQFENAKLPPAPEEDDQMEVLLRPGLLADVEIIVEKIPNAIYIPSQAVFEKDGK
                     LIAYVMQGSRFEERVLKPLKRSESTMVISEGLEPGEVVALADPNVRPGDRKKDSEKKS
                     PGGSPMGGMSKAGGA"
     gene            6902..8158
                     /locus_tag="VFQ79_10750"
     CDS             6902..8158
                     /locus_tag="VFQ79_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0140184.1"
                     /translation="MSGITGYLPEIYMGLSSLLVHKLRSLLTMLGMIFGVGAVVAMLS
                     ITAGAQKEMMSYIDLLGVNNIIIEAKEAVDRNELQARRAISPGLTFRDFRAIAENVQG
                     IEALTPRKRFKPLKMLPKTAQEPPLLIGVAPNYLEINNLRVVEGRFFNGEDNAQSVPV
                     CVLGESAKINLLGYEPAVGKSVKVNDTWLQVIGVLAQQATADTDIEGLEVTNRNNLVI
                     APLNTVMRRFEDNNSYLKDEIDGIYIKVAKGTDSIETASVVSAILTATHKDAGDFNIT
                     VPAGLLEQRRRTQFIFSVVMICIAGISLLVGGIGIMNIMLATVLERTREIGIRRAIGA
                     RQADIIRQFLTEAVLISILGGIIGVVFGYTLSKVIAAAAGWSTEVTVSSIAVAFGVSC
                     GIGLLFGIYPAVQAAKLDPIEAIRYE"
     gene            8166..9878
                     /locus_tag="VFQ79_10755"
     CDS             8166..9878
                     /locus_tag="VFQ79_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0140185.1"
                     /translation="MRSKFFLFLIMGGVPLLAQVGATGVPLPEINPPAIGSSNDAPLF
                     PTPAYFRKHFSTPPARVELRPPVRLDDFVVGDRLELSLRSFLELVMANNTDIEIQRVS
                     METFQNAIQRAFGPFDPRLVATFQSQRTKTQTGDVLAGAATLSELSQPARFTYSQTLD
                     TGTQFNIGFNATKRSTNNTFANFNPLLNAQFGMDFAQPLLRNRGRYMNRMNVTIARSR
                     LRQGEYNLEDQVLRLIVQAENAYWQVILDRENLDVQEKALEVNEQFLKRNERELELGA
                     ISPLDIYQPQARFQQFKIQVTQAQYRLEQSIDALRRQIGVDMDPRFRKIPIVLTETVE
                     PPLTTGVIDAENAVEKALRFRQDLKATVQGLDINDLQIKQNSNQLLPDLALTGAYTST
                     GRGGIFYDRENVFTELGSTSQIVQVIPGGFGDAMSQVFGFGYPIYSFGLRLTLPIRDR
                     AASANLADAMVNKRLTALRARSLEQQIRQEVLNAISQVESSKASVDLSKVQLQLAQQT
                     LDAENKKYELGTNTIFFVLDAQQQLVQARSQLVQQTVQYRRNQLNLLQRTGELLDERG
                     IAVR"
     gene            9886..11838
                     /gene="mutL"
                     /locus_tag="VFQ79_10760"
     CDS             9886..11838
                     /gene="mutL"
                     /locus_tag="VFQ79_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU0140186.1"
                     /translation="MGRIRILSDTVANKIAAGEVVERPASVVKELLENSLDAGASSVR
                     IEIESGGRRLIRITDDGCGMLADDAMLAFERHATSKLSDVKDLDSIETLGFRGEALPS
                     IAAVSRLTLETRSKEESTGARVEIAGGRMLKCEEAALASGTVVTVRDLFFNVPARKKF
                     LRTEQTELAHIASLATHYSLAHPDKTFQLLHGASELLHVTSVVELRDRVYQVFGGQTL
                     EELVDLGIRERDLQVAGAEGTETVSFFLRGFVSRPQVQKLNRNSIFAFVNGRLIKDRL
                     LLHALSAAYHNLIPPSCYPFALLFVDCDAGEVDVNVHPSKTEVRFRHGSFVHDFVRDS
                     IRAVLMESRPAPVFPLPAQAQPVASLPYSEFTQRIENQDFGPVPPAVPPGDEEFPEVA
                     LERRPAGPQRFDFSAEPLGIPPAEVREERGPDNTLVSSAPVGGPLPDSHGPLAPGPML
                     DEQQNLAVLRDLRPLGQIHDSFIIAAGRDGLWIIDQHVAHERILFERILRERAAGRVE
                     MQQLLLPIVIEVTAGQQIEYARIADELNSMGFETEPFGNRTVAIKAAPAGVGTAEIEK
                     ILYEVLETSERELRRASLDDLRRGIAASIACRAAIKINTRLDRNKMEWLLDALAGTEY
                     PMSCPHGRPIALRYSMRDILKGFHRI"
     gene            complement(11851..12180)
                     /locus_tag="VFQ79_10765"
     CDS             complement(11851..12180)
                     /locus_tag="VFQ79_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0140187.1"
                     /translation="MSQFAAFALGLCLAVSPAFSQSKSKAGDAEKGKEVFDQCGVCHY
                     PDKTEKKMGPGLKGLFKAEKMVNGKKPTETNVREIIDKGGNGMPAYEEILTSEEKDNV
                     IAYLKTL"
     gene            complement(12319..>12937)
                     /locus_tag="VFQ79_10770"
     CDS             complement(12319..>12937)
                     /locus_tag="VFQ79_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0140188.1"
                     /translation="ANGLGILWTYNYNRERTTDFFNDVDRYADDLTFIDSNNPRHRMN
                     IAGTYDIPVGKGRPLLSQANPVLNAIAGGWSFSWIFMYNSGEFLRFPQAIVSGDPAIS
                     NPTRSQYFDTTKFAPPEPYTPRTNPWQYPGVTGPRYVNLDATLAKFFPITERFRLELR
                     WEAYNLTNSFMAANPSVDIYSSLFGKSVNQVNRGREMQYTLRLHF"
BASE COUNT         2703 a         3646 c         4032 g         2556 t
ORIGIN      
        1 aggagtgctg tcgcggcggc gaaagctgta agtatgccgg cggacaacat caagcgtgcg
       61 atcatgcgcg gcacgggcga actcgaaggt ggccagatcg atgaagtaac gtttgagggc
      121 tatggacccg gcggtgccgc cgtgctggtc agcgtcgcaa cggataaccg caaccgcacg
      181 gtcagcgaga tccggcacgt attttccaaa aacggcggaa acctggggga gcagggcagc
      241 gtcgcctgga tgttcgagag aaagagccag attctgattg agcgcgacaa ggcgaacgaa
      301 gacgacctga tggcgctggt gctggatgcg ggagcggagg accttcgcga cgatggctcg
      361 aactgggagg tgctttcggc ccccgaagcg cacgatgccg tgttcgaggc gatccaaaaa
      421 gccggcatac cgacggtctc cgccgaaatc gcgatgattc cgaaaaacct cgtgaaactg
      481 gaaggcaaaa acgcggccgg catgctccgg ttgagcgaag cgttggaaga gcacgacgac
      541 gtgcagagcg tatactccaa tttcgatata gacgagaagg agttggaggc attggcgtaa
      601 gctgcgcccg ggccggaatg cgtgtgcttg gcatcgactg cggaagcgaa cgtacgggtt
      661 acggtgtgat cgagaccgac ggcaagacgc acgaactgct ggcggctggc gtcatccgga
      721 ccagcgcgaa ggaagcaccg gaaaagcggc tgttgagaat cgcaatggga ctgcgagagg
      781 taatggaaaa attctcgccc caggcggcgg cggtggaaga agtgttccac ggcgtgaatg
      841 caagagctgc ccttaagctg gcgcaagtgc gaggagtggc cctcttgacg gctgccgaag
      901 ccggcgtccc cgtgggcgaa tactctccgc tcgagattaa gacaagcgta gtcggctacg
      961 gccgggcaga aaagcggcag gtgcagttga tgcttcaatc gctgttgcac ctggaccggc
     1021 gtctggattc cgaggatgcc tcggatgccg tcgccgtcgc cgtatgccac gccactcgtt
     1081 ccgccgccct gcttcgaacc gcgttttccg gcccgggggg tgaggcatga gatatctggt
     1141 attcctcttc gccgtcgtag cggcgctggg tgcggatttg ccccgcatca cctacagcaa
     1201 gagctttcct ggaagcgtcc cgccttacgt tgaaatcagt gtcgacaaga acggtatggc
     1261 ggtttacaaa gagtctgccg acgatgagag ccccctgaag ttccggttga ccgaggcgga
     1321 ggcatccgag gtgttcgcgc ttgccgcaaa actcgaccat ttccgccggc ctctcgagtc
     1381 cggtctaaaa gtagcgaaaa tggggaaaaa gagcttccgc tacgaagacg gcaatgtcaa
     1441 aaatgaggtg cacttcaatt tttcccagga cctggatgcg cgaacgctca cggactggtt
     1501 tgagcgcatg accgaaaccg agcagcagtt cattaatctg cagcgaacga cgcgtttcga
     1561 caagttgggc gtgaaccagg cgctgatcca attgcaggcg gcgcacgagc ggaaacgcct
     1621 ggtggcgccg gaacagtttc ttccgttgtt ggaacgtatt gtaaagaacg atacttatat
     1681 gcacatgtcg cgggaacgcg ccgccggtct ggccgaggct tttcgcgcct ccacagccgg
     1741 caagtctggt gccgagtagc cactccggct tccgctatac tggaatttaa acactcacaa
     1801 cgaaaagcac tgcggagagt tacttttttc agagttggtt cgtcactata gtccagagta
     1861 ggagtgtatg aatccgcata accgaagtgc ggatgcgtgg ggtggagttt tctgatgcag
     1921 tatcagatga gtggtgcctc ggtatcggtg ttgaagccgg aagcgcctgt tctgcctgcc
     1981 ggtccgcgtc aagtcgcgcc caagcgtccg cgtaaccgca agattctgtt cctgttgctt
     2041 ctgattgccg tgtccgtggc gattggcgcc tttttgtggt ggcgggggca gcgccagcaa
     2101 tccgtggcgc aacgcgccca gatatcggcc gttcgttcgg ctccagtggg ttggggcacg
     2161 atgcaaagga ccatccgctt atctggtacc accgcggcgg cgcgttcagt cctcctgatc
     2221 gctcccagcc ttcgcggcag ccgcggcgat cggggacgat ccggcagagc agggggggcg
     2281 agcggaagca ataccactca agttgcttcc acggccactt cgacgggtag cggatcttct
     2341 ccttcgactc ctacggtctc ctcaacgggg acgggaacac cgcagactgc ctccccgggg
     2401 caggcatcgg gcggctccgg cttcgcaggc tccagcggaa cttcttcttc cggcgccttc
     2461 cgatcggcga ccagccgctt cggcggcact acgcgttcct cgcgcagctc tgcttcgcaa
     2521 agcacctcca gccagcgcag ctcacttgtt acgggtgaca cgggctccac atcctcccaa
     2581 ttgcctggcg gcggaggggg gggcggtggt ggcggcgggg acttttcgct ggttttgcag
     2641 cagttgatca agcctggcag ccacgtgaaa aagggcgaga tgctcgccga gttcgatcgc
     2701 cagtacatgc tgttgcggct tgacgattac cgcgtctccg tcgatcagca gggaaaagaa
     2761 ctggtgacgc tgaaggccaa tctcgacgta gcaaaaaaag cgcatgagca aaaaatcgtg
     2821 gcggccaaag gcgatctgga tcaggccgct ctggatctca aaacgttgcc ggtgcgctcc
     2881 gccattgatt ccgagcggct gaagctggcg catgaagagg ccctggcgcg gtataaacag
     2941 ttgctctcgg aggtaaagca tttcgaacgt tcacagcagg cccagtaccg tattgcggat
     3001 ctggacttcc ggcagtcgca actcgaattg caacggtccg aggccaacac gaaccggatg
     3061 gtcgccaatg ctccgataaa cggcctcacc gttatgcaga atacattccg cggaacggag
     3121 ttcggacaga ttcaggaagg cgatcagttg cggccggggc agccctacat gcaggtggtg
     3181 gatgtgagtg cgatggtcat tgaggctaag gtcagccagg ttgacgtgga gcatctgcgt
     3241 attggcgcaa aggcggttgt gcggttcgac gcctatccgg acctggtatt gccggctcat
     3301 gtcttttcga tcggtgcgat gacgaatcct ggcggcatgc gtgccagttt tgtgcgtgag
     3361 gtcgcggtaa agttgaggct cgacgaactc gatccgcgcg ttattcccga tctttccgtc
     3421 agcgcggatg tggtgatcga aacggctccg gagccttcca ccctgattgc gcgggaatcg
     3481 gtttttcgcg attcggccga taccgatccc tatgtctttg tacgcgccgg gaccgcgtgg
     3541 gtacggcgta atgtagaact tggtctggcc aataacatca gtgtggccgt cacttcaggg
     3601 ctcgagaagg gcgaagttgt ggcgctatcc cgtccgccgg ccgccagtaa agggcagtag
     3661 aacagttacg atgtcgaagg atctaaaacc caagcaccgc ctcgggcagg tagaatcgtc
     3721 ccgcaagaaa aagatcatcg tcatagccgt actggtggcg cttgccgtca ccggcggcta
     3781 cttcgcctac agacgcacga acacgaccga ggttgaagtt cctgtggctc gtgtccggac
     3841 cggcgaattc atcatcgccg tccgtgccag aggcgaaatc cgcagcactc gttccatggt
     3901 gctcacggcg ccccaggtgc cgaatccgca gattgtaagg ctcgccgaat ccggaaagcc
     3961 cattcgaaag ggcgaggtgg tagtggaatt cgacgctgcc gcgcaggagc aggcactgct
     4021 ggaacgaagc acttcggttc gtaccgcgga cagtgagatc gtccaaacca gggccgctca
     4081 tcgcatcacc gatgagatgg atgcgatgaa cctgatgacg gccggttata atctggagcg
     4141 cgccaagctg gaagcgagca aggcggagat cctgtcggag attgaaggcg ccaagaaccg
     4201 gatcgaagtt ggtatcagcg agggagagtt ggggcaggta aaaacaacga tcggggcgca
     4261 caaaaccacg caaaaggcgg acctggagcg gcttcagcag cgcaaggata aagcggtgcg
     4321 ggatatggac cgcgccaagg gctacctgtc gcgaatggtg attcgcgcgc ccaacgacgg
     4381 tgtggtcaac attcttccga atttccgcgc tccgggatcg ttcggctcct caccaccccc
     4441 gttcaaagaa ggagaccgtg cgtggaccgg cgctgccatc gccgaaattc cggatctgtc
     4501 ggagatgcgg atcgagctca agctggatga ggtagatcgc ggcaagatcg ccctgggcca
     4561 gcccctgcga atccgcgtgg atgccatccc ggacaaggaa ttttccgcga acctggactg
     4621 gatcagcccg atcgccgcgc tgaacttccg gggattcgga ttgaccgaaa agctgtttcc
     4681 cgcgcgcgcc actcttaaga atcttgattc ccggctccgg cccggtatga gcgcgagtgc
     4741 cgacattatc attgagcggc agtccaagca gttgctgatt cccgcgcgag cgagtttcat
     4801 gcatgcaggc aaacctgctg tttacgttca gagagggaaa gattttcaga tacgccaaat
     4861 cgaggtgggg aagcgtaacg attccgacat catcgtattg aacggactca aagagggcga
     4921 aatcatcgct ctcgagaacc ccatcgaagc ggcgaagaaa gctaagaaat tgtagccgta
     4981 tgcccgcccg ccgttattcg ttcaagaaaa ttctgatccg caccggcatt tttgctgttg
     5041 tgctggccgc ctgctggggc ggctacgtac tggtccagca gatgccgcag cggcagcaac
     5101 aggtggccac gacgaaggtt cgcaagggcg atgtcgttgt gcgcagttac gctcgtggag
     5161 aattgcaggc tgtgcgttcc gcctccctcg tggcgcccaa tctttttggg acagttcagg
     5221 tgacgaggct ggcatatctc ggcagcttcg cccgcgaagg agacctgatc gtcgaattcg
     5281 acgattctga agtgcgggcg cgccttgaag aaaagcagtt ggaactcgat caggtggacg
     5341 agcagatccg gaaggcggaa gccgatctcg ccattcgcaa caaccaggat caggtggaat
     5401 tgctgaatgc gcgatactcc gtccggcgcg ccgaactcga ggtgaagcgc aacgaactgc
     5461 tttcggagat cgacaggaag aagaaccttc tgacgcagga cgaggctcag cgccggctga
     5521 agcagctcga gagcgatatc aagtcgcggc tggagcagtc caaggcggag atggccgtgc
     5581 tcttcgagcg caaaaacaaa gccgtgctcg aaatggcgcg ggaacggcag cggctgggcc
     5641 tggtgaagct gcttgcgccc atgagcggcc tggttgccat ccgtcaaaat ttttcgggtg
     5701 gattccgcat gttcggcatg caaattcccg atgtgcgcga gggcgaccag gtgtcgcccg
     5761 gaatgctggt agccgatgtc ctggatttgt cggagctcga gatcagcgcg aaagtcggtg
     5821 agctcgaccg cgccaatctt cgcgagggcc aggatgtcct ggtgcaattg gacgcgatcc
     5881 cggaaaagca gttcacgggc aggatcaaat cgatgagcgg gacggcgagc gcgaacttct
     5941 tttctgcgga cccggccaag aaattcgacg tcacgttctc gatcgacatg aagaccctga
     6001 tgtccgctct tggcgccaag tccgagcaga tcgccagagt gctggcgacc gcggccaaga
     6061 accgcggcaa accctcgaca ccggcgccgg tcccgatggc agggatggcg gcccccggtg
     6121 ttcccccgcc cggggcaatg gccggaggtg ggatggtggg atcttcgatg ggtggtggtc
     6181 ctggcgttgt gggaggggac gttgctccgg gtgccgctgg tgcacagggc ggccgacggg
     6241 cttttgcggg cggaggtggt ccctcgatgt cggatggcga ccggcagaaa atgcgcgaag
     6301 ccatggagaa ggcgctcaaa ggccgcagca tccaggacct ttcaccagag gaacgccaga
     6361 aggtttttgc ggaggccagg aaagccgcag gtttgggcgc aggtagcccc ggcgggcggc
     6421 agcggcgtga tggtgaagcc ggccccgggg gaccgtccgc tccgatgccg cgtctgccgg
     6481 caatggtata tggcggctct tcgatattca cgcaggtgca gttcgaaaat gccaagctgc
     6541 ctccggcgcc cgaagaagac gatcagatgg aagtgctgtt gcggcccggt ctgctggctg
     6601 acgtcgagat catcgtggaa aagatcccga acgcgatcta tattcccagc caggcggtgt
     6661 tcgagaaaga cggaaagctg atcgcttatg tgatgcaggg gagccgcttt gaagagcgcg
     6721 tattgaagcc gttgaagcgc agcgaatcca ccatggtgat ctcggaaggc ctggagcccg
     6781 gcgaggttgt ggcactcgcg gatccgaacg tcagaccggg cgaccggaaa aaggatagtg
     6841 agaagaagtc ccccggcgga agcccgatgg gcggaatgtc gaaagccgga ggagcgtagg
     6901 aatgtcaggt attacaggct atttaccaga gatctacatg gggctttcga gcctcctggt
     6961 ccacaagctc cgaagccttt tgaccatgct cggcatgatc ttcggcgtgg gcgccgtggt
     7021 ggctatgttg tccatcaccg ccggcgcgca aaaggagatg atgagctaca tcgacctgct
     7081 cggggtgaac aacatcatca tcgaggcgaa agaggcggtg gatcgcaacg aacttcaggc
     7141 ccggcgcgcg atttcaccgg ggctgacctt ccgggacttc cgtgcaatag ccgagaatgt
     7201 tcagggcatc gaagcgctga cgccgcgcaa acgttttaag ccgctgaaga tgctcccgaa
     7261 gacggcgcaa gagccgcctc tgctgattgg ggtcgcgccg aattacctcg aaattaataa
     7321 cctccgtgtg gtggaaggac ggttcttcaa tggcgaggac aatgcgcaat ccgttccggt
     7381 ctgtgtgctg ggggaaagcg ctaagataaa cctgcttggc tacgaaccgg cggtgggtaa
     7441 atccgtcaaa gtaaatgata cgtggctgca agtaattggg gttctcgccc aacaggcgac
     7501 tgccgatacg gatatcgaag ggctcgaagt aaccaaccgg aacaacctgg tcattgctcc
     7561 gctcaacacg gtgatgcgcc ggtttgaaga caacaacagt tacctcaaag acgagatcga
     7621 cggcatttat atcaaggtgg ccaaagggac cgattccatt gagacggcca gcgtggtgag
     7681 cgccatcctg acggcgaccc acaaggatgc cggcgacttc aacattacgg tgcctgccgg
     7741 actgctggaa caaaggcgcc gcacgcaatt catcttcagc gtcgtgatga tctgtatcgc
     7801 cggaatttcg ctgctggtcg gcggtattgg aatcatgaac atcatgctcg caaccgttct
     7861 ggagcgtacg cgcgagatcg gtatccggcg cgccatcggt gcacggcagg ccgacatcat
     7921 ccgtcagttc cttacggagg ctgtcctcat ttccatattg ggcggcatca tcggtgtggt
     7981 tttcggatac acgttatcga aggtcattgc ggcagcggca ggctggtcca ccgaagtcac
     8041 ggtttcgtct attgccgtgg cttttggcgt gtcctgcggc atcggactgc tgttcggaat
     8101 ttatcccgcc gtgcaggcgg ctaagctgga tcctattgaa gcgattcgtt acgagtgagt
     8161 tagagatgcg ttccaagttc tttttattcc ttatcatggg tggagttccg ctgctggcgc
     8221 aggtcggagc taccggggtg ccgcttcccg agatcaatcc gccggccata gggtcgtcca
     8281 acgacgcgcc cttgtttccc acgcccgcct atttccggaa gcacttttcc acgcccccgg
     8341 caagggtgga gttgcggcct cccgtccggc tggacgattt tgtggtgggc gaccggctgg
     8401 aactttccct tcgctccttt ctggaactgg tgatggcgaa caacaccgac atcgagatcc
     8461 agcgtgtctc catggaaacg ttccagaatg cgatccagcg tgcctttggc cccttcgatc
     8521 cgcggctggt cgccactttc cagagccagc gtacgaagac gcagaccggt gatgtgcttg
     8581 ccggcgccgc gactttaagc gaactctcgc agccggcacg gttcacgtac tcacagacgc
     8641 tcgataccgg tacgcagttc aacattggtt tcaatgccac gaaacgctct acaaataata
     8701 ccttcgccaa tttcaacccg ctgctgaatg cgcagttcgg catggatttc gctcagccgc
     8761 tgcttcgcaa ccgcggccgg tacatgaacc ggatgaatgt cacgattgcg cgcagccgtc
     8821 tgcggcaggg cgagtacaac ctggaagacc aggtcctccg gctgatcgtc caggcggaga
     8881 acgcatattg gcaggtgata ctcgaccgcg agaatctcga cgtgcaggag aaggccctcg
     8941 aagtgaacga gcagttcctg aagcgcaatg agcgggaact ggagctgggc gcaatttcgc
     9001 cgctcgatat ctatcagccg caggcgagat ttcaacagtt caagatccaa gtgacacaag
     9061 cgcaataccg cctggaacaa tcgatcgacg ctttgcgccg ccagatcggc gtggacatgg
     9121 acccacggtt ccggaagata ccgattgtgc tgactgagac cgtcgagcct ccgctcacta
     9181 ccggagtgat tgatgctgaa aacgccgttg agaaggcgct ccggttccgc caggacctga
     9241 aagcgacggt gcaaggactc gacatcaacg atctccaaat caaacagaac agcaaccagc
     9301 tcttaccgga tcttgcgttg accggcgctt acacttccac tggccggggg ggcatttttt
     9361 acgaccgtga gaacgtgttc acggaacttg gtagcaccag ccagattgtt caggtcatcc
     9421 ccggcggctt tggcgacgcg atgagccagg tgttcgggtt cggatacccc atctattcgt
     9481 ttggcctgag gctgacgctt ccgatccgag accgcgcggc cagcgctaat ctcgcggatg
     9541 cgatggtgaa taagcggctc accgcactgc gtgcccgcag cctggagcag cagatccgcc
     9601 aggaagtgtt aaacgcgatc agccaggtgg agtcgagcaa agcgagcgtc gatctctcca
     9661 aagtccagct ccaactggcg cagcagacgc tggacgccga aaacaagaaa tacgaactcg
     9721 ggacgaatac gatcttcttc gtcctggacg cacagcaaca gttggtacag gcgcgaagcc
     9781 agttggtaca acagaccgtg cagtaccgcc gcaatcagtt gaatttgctc caacgaaccg
     9841 gagagcttct ggacgagcgc ggcatcgcgg tacgatagcg gctgaatggg ccgtatccgg
     9901 atcctctccg acaccgtcgc caacaagatc gcggctggag aggtagtaga gcggcccgca
     9961 tcggtcgtca aggagttgct cgaaaactcg ttggatgcgg gcgcttccag cgttcggatc
    10021 gagatcgaga gtggcggccg caggctcatc cgcatcacag acgacggatg cggcatgttg
    10081 gcggacgacg ccatgctcgc cttcgagcgg cacgctacca gcaagctcag cgacgtgaaa
    10141 gacctggact ccatcgagac tctgggtttt cgcggcgaag cgttgccctc cattgccgct
    10201 gtctcgcggc ttacgctgga aacccgttcc aaagaggaat ccaccggtgc gcgcgtagaa
    10261 attgccggcg gcaggatgct caagtgtgaa gaggctgcgc ttgcatccgg cactgtagtg
    10321 acagttcgcg atctgttctt caacgtgccc gcgcgtaaga agttcctgcg aacggagcag
    10381 acggaactgg cacacatcgc ctcgctcgcc acccattaca gcctggccca ccccgacaag
    10441 acctttcagc ttctacacgg tgcgagtgag ttgcttcatg tcacatcggt tgtcgagctc
    10501 cgcgaccggg tctaccaggt attcggaggg cagacattgg aggaactcgt agacttgggc
    10561 attcgcgagc gggaccttca ggtcgccggc gccgagggca cggaaaccgt cagtttcttc
    10621 cttcgcggct tcgtctcccg gccgcaggtg caaaagctga accggaactc catattcgcg
    10681 ttcgtcaatg gacggctcat caaagaccgg ctgctgttgc atgctctgtc ggcggcgtat
    10741 cataacctga ttccgccgtc gtgctacccc ttcgcgctgc tgtttgtcga ttgcgacgcg
    10801 ggcgaggtgg atgtgaacgt acatccttca aaaacggaag tacggttccg gcacggctct
    10861 tttgtccatg actttgtgcg ggattccatc cgggccgtgc tgatggaatc ccgtccagcc
    10921 ccggtgtttc ctctgcctgc ccaggcgcag ccggtggcct cgctgccgta ttccgaattt
    10981 acacagcgga tcgagaatca ggacttcggc cctgttcccc cggcggtgcc gcccggcgat
    11041 gaagagttcc cggaggttgc ccttgaacgc cggccggcgg gtccgcagcg gttcgatttt
    11101 tccgccgaac cactgggaat cccaccggcg gaagttcgag aggagcgtgg gccggataac
    11161 acactcgtct cttctgcacc ggtcggtggt ccactgcccg attctcatgg gccgcttgcg
    11221 ccggggccga tgctggatga gcagcagaat ctggccgtcc tcagagacct gcggccgctc
    11281 ggacagatcc atgacagctt catcatcgcg gcggggcgcg atgggctatg gattatcgac
    11341 cagcacgtcg cgcacgaacg cattctgttc gagcgtattc tgcgcgagcg ggcggcggga
    11401 cgcgtggaga tgcagcaact gctgctgccc atcgtcatcg aagtaacagc cggacagcaa
    11461 atcgagtacg cgcgaatcgc cgatgagttg aactccatgg gtttcgagac ggagccattc
    11521 ggcaaccgga ccgtggcgat taaggcggcg cctgccggag tcggcacggc ggagatcgaa
    11581 aagatcctgt acgaggtcct ggaaaccagc gagcgcgaat tgcggcgggc ttcgctcgac
    11641 gacctgcgcc gcggtatcgc cgcatccatc gcgtgccgcg ccgctatcaa gatcaatacc
    11701 cggctcgatc ggaataagat ggaatggttg ctggatgccc tggcaggcac ggagtacccg
    11761 atgagctgcc cacacgggcg tcccatcgcg ctccgatact caatgcgcga tattctgaag
    11821 ggctttcatc gcatctgaca cccgcgggct tcagagtgtc ttcagatagg cgatgacgtt
    11881 gtccttttcc tcgcttgtga ggatctcctc gtaagccggc atgccgttgc cgcccttgtc
    11941 gatgatttcc cggacatttg tttcggtcgg ctttttacca ttcaccattt tttcggcctt
    12001 gaacaggcct ttcaagccgg gacccatctt cttttccgtc ttgtcgggat agtggcacac
    12061 gccgcactga tcgaagactt ccttgccttt ttcggcatcg cccgctttcg atttgctttg
    12121 cgaaaaagcg ggggaaacgg ccaggcacag acccaacgcg aaagctgcaa attgcgacat
    12181 cctcatgtcc tacgggagct cctcttagcc atggaaagaa atgattttct tcagtttact
    12241 acaacccgcc tcaccgtcat cggaagcaca cggcgcgccc ggcggaccgg gccgggcgcg
    12301 ccgatcgcgc tggctcgatc agaagtgcaa ccgcagcgtg tactgcatct cgcgtccgcg
    12361 gtttacctgg ttcacggact ttccgaacaa cgagctgtaa atatcgacgc tcggattcgc
    12421 cgccatgaag ctgttggtca ggttataagc ttcccatcgc agctcgaggc gaaaccgctc
    12481 cgtgatcggg aagaacttcg cgagcgtggc gtcgaggtta acgtagcgcg gcccggtcac
    12541 accagggtac tgccagggat tcgtgcgtgg cgtgtagggc tctggcggcg cgaacttcgt
    12601 cgtatcgaag tactggctgc gggttggatt tgaaatggcg ggatcgccgc tgacgattgc
    12661 ctgcggaaac cggaggaact cgcccgagtt atacatgaaa atccaactga acgaccaccc
    12721 gccggcgatg gcattcaata ccggattggc ttgcgataac agcggccggc ctttccccac
    12781 cgggatgtcg taggttccgg cgatgttcat ccgatggcgc ggattattgc tgtcgataaa
    12841 cgtcaggtca tcggcatatc ggtccacatc attgaaaaaa tccgtggtcc tttcgcggtt
    12901 gtagttgtac gtccagagaa tgccgagtcc gtttgcg
//
LOCUS       DASTMK010000248         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_427651, whole genome shotgun sequence.
ACCESSION   DASTMK010000248 DASTMK010000000
VERSION     DASTMK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427651"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFQ79_10775"
     CDS             complement(<1..312)
                     /locus_tag="VFQ79_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0140189.1"
                     /translation="MRSGEAVKTCIESRAKTILLVEDSPGDVRLTREALRESSAQVVL
                     EVARDGVEAMEMLRSESTRTGRFPDLILLDLNLPRKDGREVLAEIKADPHLRLIPVVV
                     LT"
     gene            470..1111
                     /gene="recO"
                     /locus_tag="VFQ79_10780"
     CDS             470..1111
                     /gene="recO"
                     /locus_tag="VFQ79_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU0140190.1"
                     /translation="MPARVSESILLRTYPLREADLIVSFLTRDQGKLRGVARAARRPK
                     SRFGAGLERLSHVRMFYFQRENRELVTLDSCDLIRSAFDTAPDYETGVAMDFLAEVSE
                     QILPAAEPNERHFRLLLAMMEHLRPGGNDRIWSAVTYFSLWSVRLAGVLPELRLASES
                     RGIAEEMFRTPIGQLAPRAWDRTTAADLRRSLVRTIEDHIERKLLSAPHLESL"
     gene            1114..2019
                     /locus_tag="VFQ79_10785"
     CDS             1114..2019
                     /locus_tag="VFQ79_10785"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU0140191.1"
                     /translation="MDSFQQVIFKLKNYWSERGCIIQEPYDVEVGAGTMAPETFLRVL
                     GPDPYHVAYVQPSRRPADGRYGDNPNRLYKHQQLQVILKPSPGDIVDQYLASLEAIGI
                     DLHKHDIKLEEDNWESPTLGAWGIGWQVMLDGLEITQFTYFQQCGGIDLDLVPAELTY
                     GLERLVAFLQGVESVYDIEWTPGVKYGAVRHADELQNSVYNFELADISTLWQLFALYE
                     GECARLMGMYAGYEEKRRFPVLSAYDLVLKCSHLFNLLDSRGAISVTERVGIIGRVRK
                     LAVGVAQAWVEQQSIGVPQTAEVSG"
     gene            2016..4010
                     /gene="glyS"
                     /locus_tag="VFQ79_10790"
     CDS             2016..4010
                     /gene="glyS"
                     /locus_tag="VFQ79_10790"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU0140192.1"
                     /translation="MRLPFLLEIGTEEIPDWMIEPSLRELAGSFAALLSANGLSGAAV
                     AVEATPRRLTLRADRIPDRQPDSVEAISGPPKSAGDAAAAGFARKAGISVQDLEVVTT
                     PKGEYFQYKKRIEGRRTIDIFAGALPGLILGIPWPKTMYWTGKTGPRFIRPIRWLVSL
                     LGKDVVPFEIAGVSSGNITSGHRRLGKKKIRITCRSYPEMLRKNYVLLSAAERREKIE
                     TEIAGLLEGTGLRVKKDAGLLNTLAFMTEYPTPILGRFDESYLRLPEEVLVTVMRHHQ
                     RYFSVEDASGALAPHFIAVMNTSADADGLVQAGNERVLKARFNDAAFFWAVDQQKKLR
                     DRVGDLANVTFQAKLGSYKDKTERMVQLVRDLGGPEAAIRAAELSKCDLTTGMVKEFT
                     ELQGVVGGLYARAQGEGEDVALAIYEHYRPVSMEDAIPGSPDGQLVSLADKLETLRGC
                     FGIGLIPTGSGDPFALRRAAQGVVRILVEGRCEFSLRKLAAGDEKLLEFLLDRARYYF
                     RDVKGFKYDEVNAVLAAGYDDLVDAASRLEAIQRVRPTENFEPLAASFKRIQNILRQA
                     GSSAGQEFRPELLEAGPERDLYEEFERVRSGVEEHRRGRAYLSALEAIASLRSKVDLF
                     FDKVLVNAPDPEIRRNRLTLLHNLLTEFSAIADFSEIVTS"
     gene            4058..>4671
                     /locus_tag="VFQ79_10795"
     CDS             4058..>4671
                     /locus_tag="VFQ79_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU0140193.1"
                     /translation="MKKYVYSFGGGTADGDGSMKDTLGGKGAGLAEMSRAGVPVPPGF
                     TISTEVCNIYFSENQQVPKEVQKQVDEALAKLEKAMGQKLGDTNDPLLVSVRSGSKFS
                     MPGMMDTILNLGLNDRTVEALVSKSGNPRFAWDCYRRFIQMFGSVVLEIPKSEFERLF
                     DGRKHKVKAKLDTDLTAEDLQAVVADYKKLVQKLTKKPFPQDAR"
BASE COUNT          982 a         1276 c         1461 g          952 t
ORIGIN      
        1 agtgagcacg acaacaggga taagccgcag gtgagggtcg gctttaattt cggcgagcac
       61 ttccctcccg tcttttcggg gcaagttcag gtcgagcagt atcaggtcgg gaaaacggcc
      121 ggtacgcgtg ctttcgctac ggagcatttc catcgcttcc acaccgtcgc gggccacttc
      181 gagaacaacc tgcgccgagc tctcccgaag cgcttcccgc gtaagacgga catcaccggg
      241 gctgtcctcc accagcagaa tcgtcttcgc tctggattcg atacaagtct taactgcctc
      301 cccacttcgc attacttttc cgtcgatttt gtaagacgcg tgcgttcgaa tttggtgtca
      361 gatatcgaga acccgttccg gctctctttg aatcttccgg acctgatcgt gcggaattca
      421 cggggttgga tgtgataagc tggatgcttc aacgccggtt agccgaaata tgccggcccg
      481 ggtaagcgaa tcaatccttt tacgaacgta tccactccgc gaagcagacc tgattgtcag
      541 cttcctgacc cgggatcaag gtaaattaag gggcgtggcc agggcagcca ggcggcccaa
      601 gagcaggttc ggagccggcc tggagcggct ctcgcatgtg cggatgttct attttcagcg
      661 agagaaccgc gaactggtta cgctggactc ctgcgatctg atccggtcgg cattcgatac
      721 ggcgccggat tatgaaacgg gagtggccat ggattttctg gccgaggtgt ccgagcaaat
      781 tctgccggcg gccgagccga acgaacggca tttcaggctg cttttggcga tgatggagca
      841 cctgcggcca ggcgggaacg acaggatatg gtccgcggta acgtatttca gcttgtggtc
      901 cgtgcgcctc gcgggagttc taccggagtt gcgcttggca tctgaatctc gcgggattgc
      961 cgaagagatg ttccgcacgc ccatcggaca attagcacca agagcctggg accgcacaac
     1021 cgccgccgac ctgcgccgtt cgctcgtccg cacaattgaa gatcacatcg agcggaagct
     1081 tctgagcgct ccccacctcg agagccttta aggatggact cctttcaaca agtcatattc
     1141 aaactgaaga actactggtc cgagcggggc tgtattatac aggagcccta tgacgtcgag
     1201 gtcggcgcgg gtaccatggc gcccgagaca tttctacggg tgttgggacc ggatccctac
     1261 catgtcgctt atgtgcagcc aagccgccgt cccgccgatg gccgctatgg agacaacccc
     1321 aaccggctgt ataagcacca gcagttgcag gtgattctca agccttctcc cggcgacatc
     1381 gtcgaccaat acctggccag cctggaggcg atcgggatcg acctgcacaa gcacgacatc
     1441 aagctggaag aggacaactg ggagtcgccc acgctgggcg cctggggcat cggctggcaa
     1501 gtgatgctcg acggtctgga gatcacgcag ttcacttact ttcagcagtg cggcggcatc
     1561 gacctggacc tggttccggc cgagttgacc tatgggctgg aacgcctggt ggctttcctg
     1621 cagggcgtcg agagcgtata cgacatcgag tggactccgg gcgttaagta cggggccgtc
     1681 cgccatgccg acgaattgca gaactccgtt tacaacttcg aactcgcgga tatttccact
     1741 ttgtggcagc tattcgcctt gtatgagggc gagtgcgcgc gcctgatggg aatgtacgcg
     1801 ggctacgagg agaaacggcg gtttccggtg ctttcggcgt acgacctcgt gttgaagtgt
     1861 tcccatctgt tcaacctgct cgactcgcgc ggcgccatca gtgtcacgga gcgtgtcgga
     1921 atcatcgggc gagtccggaa actcgccgtc ggtgttgcgc aagcttgggt ggaacagcag
     1981 tccatcgggg ttccgcaaac ggcggaggtg agcggatgag actgccattt ctgctcgaaa
     2041 tcggaactga agagattcct gactggatga ttgagccgtc gttgagggag ctggcggggt
     2101 cctttgccgc gctattatcc gctaacgggc tctctggcgc ggcggtggcc gtcgaggcga
     2161 caccacggcg cctcaccctg cgtgcggacc ggattccgga ccggcagccg gattccgtgg
     2221 aagcgatcag cggccccccg aagagcgcgg gtgatgccgc ggccgcgggg tttgcgcgga
     2281 aagccggcat cagcgttcag gacctggaag tggtgacgac tccgaaaggg gaatacttcc
     2341 agtacaagaa gcgaattgaa ggccgccgca cgatcgatat ctttgcgggg gcactgccgg
     2401 gcctgattct tggtatcccg tggcccaaga cgatgtattg gacaggcaaa acgggacctc
     2461 gcttcatccg gccgattcgc tggctggtct ctcttctggg taaagacgtc gtgccgtttg
     2521 agatcgccgg cgtttcttcg ggaaacatta ccagcggaca caggaggctc ggcaagaaaa
     2581 aaatcaggat cacttgccgg agttatcctg agatgctccg gaagaactat gtcctgcttt
     2641 cggcggccga acggcgcgag aaaatcgaaa cggagatcgc cggtctgctg gaggggacag
     2701 gcctgcgcgt gaaaaaagat gctggcctgc tcaacacgct ggcgtttatg acggagtatc
     2761 caactcccat cctcggccgc tttgacgaaa gctatctccg tctgccggag gaggtgctgg
     2821 tgacggtgat gcgccatcac cagaggtatt tttccgtgga ggacgcttcc ggggcactgg
     2881 cgccgcactt catcgccgtg atgaacacaa gcgccgacgc cgatggcctg gtacaggcgg
     2941 gcaacgaacg ggttttgaaa gcgcgattca atgacgcggc gtttttctgg gccgtggatc
     3001 agcaaaagaa actgcgcgac cgcgtcggcg acctcgccaa tgtcacgttt caggcaaagc
     3061 tcggatccta caaagacaag acggaacgaa tggttcagct tgtgcgcgac ctcggcggcc
     3121 cggaggccgc tatccgcgcg gcggaattaa gcaagtgcga cctgaccacc ggcatggtga
     3181 aggaatttac cgagctgcag ggtgtcgtgg gcggcctcta tgcgcgcgcg cagggggaag
     3241 gagaagacgt agccctcgcg atctacgaac actacagacc ggtgagcatg gaggatgcga
     3301 ttccgggctc gcctgacggt caattggtgt cgctggcaga caaactggag acgctcagag
     3361 gatgctttgg gatcgggttg attcccaccg ggtccggcga tcccttcgcg ctgcgccgcg
     3421 ccgcgcaggg tgtggtacgg atcctggtgg aggggcggtg cgaattttcc ctgcgcaaac
     3481 tcgctgccgg tgacgagaaa ctgttggaat ttctcctgga tcgggcaaga tattatttcc
     3541 gcgacgtgaa gggatttaag tatgacgaag tcaatgccgt gctcgcggcc ggatacgacg
     3601 atctcgtaga cgcggccagc cgcctggaag cgattcaacg tgtgcgcccg acggagaact
     3661 ttgaacccct ggcggcgagc ttcaaacgga ttcagaatat tttgcgccag gcgggttcga
     3721 gcgcgggaca ggaattccgg cccgagttgc tggaggcagg ccccgagcgc gacctgtatg
     3781 aagaattcga acgcgtacgt tccggagtcg aggagcatcg caggggccgt gcgtaccttt
     3841 cggcactgga agcgatcgct tctttacgtt cgaaagtgga tttgtttttc gacaaagtgc
     3901 tggtgaatgc gccggaccct gaaatccggc gtaaccggct cacgcttttg cataatttac
     3961 ttaccgagtt ttcagccatc gccgatttct cggaaattgt cacctcataa aatagaagat
     4021 gttgaactaa tcgatagctt aggagagcac agtaaatatg aaaaaatatg tctattcgtt
     4081 cggcggcggc acggccgacg gcgacggatc gatgaaggat acgctcggtg gcaagggcgc
     4141 gggcctggcg gagatgagcc gggccggggt tcctgttccg ccgggcttta ccatttcaac
     4201 cgaagtctgc aacatctatt tttccgagaa tcagcaggtt ccgaaagaag tccagaagca
     4261 ggtggacgag gccctcgcca aattggaaaa ggcaatgggc caaaagctcg gggacacgaa
     4321 cgacccgttg ctggtgagcg tccgttccgg ctccaagttc tcgatgcccg gaatgatgga
     4381 taccattctg aacctggggc tgaacgatag aaccgtcgaa gcgttggttt ccaaaagtgg
     4441 caatccgcga tttgcgtggg actgttaccg ccggttcatc cagatgttcg ggagcgtcgt
     4501 gctggagatt ccgaagagcg aattcgagcg ccttttcgac gggcgtaagc acaaggtgaa
     4561 agcgaagctc gatacggacc ttacggccga agacctgcaa gccgttgtcg ccgactacaa
     4621 aaagctggtg cagaagctga cgaagaagcc ctttccgcag gacgcgcgcg a
//
LOCUS       DASTMK010000249         4194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_619968, whole genome shotgun sequence.
ACCESSION   DASTMK010000249 DASTMK010000000
VERSION     DASTMK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619968"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /gene="hpnE"
                     /locus_tag="VFQ79_10800"
     CDS             complement(<1..1013)
                     /gene="hpnE"
                     /locus_tag="VFQ79_10800"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEU0140194.1"
                     /translation="MSDCIVIGGGLSGLATAVALGSAGLRVQLFESRGFLGGRATSYP
                     ADIGDQTEIIDNCQHVLLRCCVNLMDFYGRLRVADRIHFYREFYFLEPGGRISYLRAG
                     HLPAPYHFAESFLTMKCLSPSDKLSIAYGMQCIRREYGRRKDLDRITMLDWLKEKRQT
                     RRGIERFWGQVLVSAINEDLGRMAARHGFQVFWLGFLRHKNSYEMGVPVVPLAELYDS
                     NAWQNLGNVEFRLRAPVERIWVERDAVYGITASKREHTARFYVCALPFERLESVIPEL
                     GIKTDLFQHSPITGVHLWFDRTVTELPHAVLLDGAVQWIFNRDQGKYLQTVISASRSL
                     VPM"
     gene            complement(1198..3357)
                     /locus_tag="VFQ79_10805"
     CDS             complement(1198..3357)
                     /locus_tag="VFQ79_10805"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU0140195.1"
                     /translation="MRQLRYTTPSGIAVTRSASKVPFKNGLEFLLRDLDYYRGFYLSS
                     GYEYPGRYSRWDIASIKPPLEVVSRGRQVEFRALNLRGRTLAKMLYPILAPHPHWESF
                     DFGAGGLYGRLKPLAGLFPEEERSKQPSVFSILRAFSTEFRNAQDSRLSLVGAFGYDL
                     LFQFDPIELKLPRDGTKDLHLFLCDDIYFMDRKKEQIERYQYDFEFGGLSTLALERSG
                     KKLAGVPSTASTGISSDHEPEEYMEKVETVRQGMARGDYYEVVLRQTFRAPYSGSPSA
                     LFERIQHLSPSPYEFLLQLGGEQLIGASPEMFVRVEGSRVETCPIAGTVRRTGDPLSD
                     AENIRELLNSFKEESELTMCTDVDRNDKSRVCVPGSVKVIGRRLIESYAGVFHTVDHV
                     EGQFQPGFDSLDAFLTHMWAVTIIGAPKKAAAQAIENLEKDARGWYGGAVGMLSLNGD
                     INTGILIRTIHLKNGIAAYGAGATLLYDSVPANEEQETRLKATGFFRALGATPEPEKE
                     SPPAPVRPLAGVRMLLVDNDDCFIHTLANYARQAGAEVVTYRAGFPIGLIPEIAPSVI
                     LISPGPGRPQDFGVAEVVRYAAEKGIPVFGVCLGLQGVVEAFGGELGVLDYPMHGKPS
                     HVTHSNRGVFEGLPARFKVGRYHSLFARRETLPDCLEITAESEDGVIMGLRHKHLPIE
                     AVQFHPESMLTLEHDCGLKLIENVVRLYGLSRQAASR"
     gene            3464..>4194
                     /locus_tag="VFQ79_10810"
     CDS             3464..>4194
                     /locus_tag="VFQ79_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEU0140196.1"
                     /translation="MKLQFFLTFALVLPLAAEVKITQKADRVQIEIDGSPFTDFFIGE
                     DAPKPYLHPLRSASGKIVTRRFPMEQIEGEVRDHPHHRGLWFSHGDVNGYDFWANERS
                     SKRPKSGLITLKKVNSVKSGSKSGSVSASFEWKEPDGKLLLTEDRTMVFYSDAKLRTI
                     DLDIKLTPAQTVKFGDTKEGCFAIRLADPLNEKHSGKMVSADGKRGESEVWGTRSPWV
                     DYHGEVDGEKLGVAIMDHPSSPRHPT"
BASE COUNT          940 a         1226 c         1205 g          823 t
ORIGIN      
        1 ctcatcggca caaggctgcg ggatgcgctg atcaccgtct gcaaatactt gccctggtcc
       61 cgattgaaaa tccactgcac ggcgccatcg agaaggacgg catggggcag ttcggtcact
      121 gtgcgatcga accatagatg cacgcccgta atgggcgaat gctggaacag atcggtcttg
      181 ataccgagtt ccgggatcac ggactccagc cgctcgaaag gcagggcaca cacgtaaaag
      241 cgcgcggtgt gctcgcgctt ggacgccgta attccatata ccgcgtcgcg ttccacccag
      301 atgcgttcca cgggagcgcg caggcggaac tcgacattcc cgagattctg ccaggcgttc
      361 gagtcgtaca attcggccag gggcaccaca ggaaccccca tttcgtaaga gttcttatgg
      421 cgcaaaaagc ccagccagaa gacttgaaac ccatggcgtg cggccatgcg tccgagatct
      481 tcgttgattg cgctgaccag cacctgcccc cagaaacgtt cgatgccgcg gcgggtctgc
      541 cgcttttctt tcagccaatc gagcatggtg atgcggtcca gatctttgcg gcgcccgtac
      601 tcgcgccgga tgcattgcat cccgtatgcg atggagagtt tgtcggacgg cgagaggcac
      661 ttcatggtga ggaagctctc cgcgaagtga tacggagcgg gaagatggcc cgcgcgaaga
      721 taagaaatac gcccgcccgg ttccaggaaa taaaattcgc ggtagaaatg aatccggtcc
      781 gccacccgca accttccgta gaaatccatc agattgacac agcatcgcag caacacgtgc
      841 tggcagttgt cgatgatttc ggtttgatcg cctatgtcag cgggataaga ggtagcgcgt
      901 ccgccgagaa acccgcgcga ctcgaagagt tgaacgcgca gtccggcgct tcccaacgcc
      961 acggcggtgg cgaggcccga caggccacca ccaatcacaa tgcagtctga cattcggtcc
     1021 ggctagctga tcggtactct tgcgcggtgt cccccgtcat caccgccgtc gataatcata
     1081 atagcgatcc aaagaacaac gatcagaaaa agcagatcgg acggagtaaa caaactttcc
     1141 attacgattc cttcttagta ttccaatacc gcttgcttgc gcgcgcggct ccgtttctca
     1201 acggctggct gcctggcggg ataggccgta caacctgact acgttctcga tcagcttcaa
     1261 accgcaatcg tgctccagcg tcagcatcga ttcgggatgg aactgaacag cctcgatcgg
     1321 aagatgtttg tgccggagcc ccataattac accgtcctcg gattcggccg tgatttccag
     1381 gcaatccgga agcgtctctc tccgtgcgaa cagcgagtga taccggccga ctttgaatcg
     1441 agcgggcaag ccttcaaaca ccccgcggtt cgagtgcgta acgtgggagg gcttgccgtg
     1501 catcggatag tcgaggacac ctaactcacc accgaacgcc tccacaacgc cctgcaaccc
     1561 gagacataca ccgaacacgg gaatcccttt ttcagccgcg taccgcacga cctcggccac
     1621 accaaagtct tgcggcctgc ccggacccgg agagatcaaa atcaccgacg gggcaatttc
     1681 cgggatcagc ccgataggaa atccggcgcg gtaggtgacc acttccgcgc cagcctggcg
     1741 agcgtaattc gccagcgtgt ggatgaagca atcgtcgttg tccaccagca gcatccgcac
     1801 ccctgccagc gggcggaccg gcgcaggcgg actttccttt tccggttcgg gcgttgcgcc
     1861 cagcgcccgg aagaagccgg tggccttgag acgggtttcc tgctcttcat tcgcgggaac
     1921 cgaatcgtac agcagcgtgg ctccggcgcc gtaagccgcg atgccatttt tcagatgaat
     1981 ggtccggatc agaattcccg tgttgatgtc gccgttcagc gacaacatgc cgaccgcgcc
     2041 gccataccag ccgcgggcgt ctttttcgag gttctcgatg gcctgtgccg ccgctttctt
     2101 cggcgcgccg atgatggtga cggcccacat atgagtgaga aaggcatcga gcgagtcaaa
     2161 gccgggttgg aactgccctt cgacgtgatc gaccgtgtgg aagacgccgg cataggattc
     2221 aatcaaccgc cggccgatga ccttcaccga acccggcaca cagacgcggg atttatcgtt
     2281 ccggtccacg tccgtgcaca tcgtcagctc agattcttcc ttgaaggagt tcagcagttc
     2341 gcggatgttc tccgcatcgg ataacggatc ccccgtccgg cgtacggtgc cggcaatcgg
     2401 acacgtctcc acgcggctgc cttcgacgcg cacaaacatc tccggcgaag cgccgatcag
     2461 ttgctcgccg cccaattgca gcaaaaactc gtatggactg gggctcaggt gttgaatgcg
     2521 ctcgaacaag gccgacgggc tgccggaata gggcgcgcgg aacgtctgcc ggagtacgac
     2581 ttcgtagtag tcgccccgcg ccattccctg gcgcaccgtc tccacttttt ccatgtactc
     2641 ttccggttca tggtcggagg aaatgccggt tgaagccgtt gacggcacgc cggcgagttt
     2701 cttgccggat ctctcgaggg cgagcgtaga gaggccgcca aactcgaaat cgtactggta
     2761 tcgctcgatc tgttccttct tgcgatccat gaagtaaatg tcgtcgcaga ggaacagatg
     2821 gagatccttg gttccgtcgc gcggcagctt caactcgatc ggatcgaatt ggaataacaa
     2881 gtcgtagccg aacgctccaa caagagagag ccggctgtcc tgcgcgttcc ggaattcggt
     2941 gctgaatgcc cgcagaatgg aaaaaacgga aggctgttta ctgcgctcct cttccggaaa
     3001 cagtccggcc agcggtttca aacggccgta aagcccgccg gcgccgaaat cgaaactctc
     3061 ccaatgagga tgcggggcga gaatgggata cagcattttc gccaatgttc gaccgcgcaa
     3121 attcagtgcc cgaaactcga cttggcggcc gcgcgagaca acttccaggg gaggctttat
     3181 ggaggcgata tcccatcgcg aataccggcc gggatactcg tagcccgagg aaagatagaa
     3241 accccggtag taatccaggt ctctcaacag gaattcgagt ccgtttttga aaggaacttt
     3301 agaagcactg cgagtgactg cgataccgct tggagtggta tatctcaact gccgcattcg
     3361 gacgccctga taaaaccgga gtgatacttc agaataccac gctgcctttc accactccgg
     3421 cgccaggttg ctctaagctt catccatgtg ttctacttcc atcatgaaac tgcaattttt
     3481 cctgaccttt gcgctggtat tacccttggc cgcggaggta aagatcactc agaaggcgga
     3541 tcgcgtccag attgaaatcg atggcagccc gttcacggat ttcttcatcg gcgaagacgc
     3601 gccaaagcca tacctgcatc cgctacgctc cgcttcgggc aaaatcgtga ctcgccggtt
     3661 tccgatggaa cagatagagg gagaggtcag ggaccatccg catcaccggg gattgtggtt
     3721 ttcacacggc gacgtgaatg gatacgattt ctgggccaat gagcgatcgt cgaagaggcc
     3781 aaagtccggg ctcatcacgt tgaagaaagt caacagcgtc aagagcggat caaagagcgg
     3841 ttcggtctca gccagcttcg aatggaagga gccggacggc aagctcctgc tgaccgaaga
     3901 ccggacgatg gtgttctact ccgatgcgaa gcttcgcacg atcgacctgg atatcaaact
     3961 cacaccagct caaacggtga agttcggaga taccaaggag ggatgctttg cgatccggct
     4021 cgccgatccg ctgaacgaga agcatagcgg aaagatggtg agcgcggatg gcaaacgcgg
     4081 agagtcggag gtctggggaa cgagatcgcc gtgggtcgac tatcacggag aagtggatgg
     4141 ggaaaagctt ggggtggcga ttatggatca cccttccagc ccgcggcacc cgac
//
LOCUS       DASTMK010000250         8109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_587894, whole genome shotgun sequence.
ACCESSION   DASTMK010000250 DASTMK010000000
VERSION     DASTMK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587894"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFQ79_10815"
     CDS             <1..397
                     /locus_tag="VFQ79_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140197.1"
                     /translation="GSLAAVTQVRNEVAQTALADLLAIMKDLGNTPAPAAELATAKNL
                     LSGTFVLSVETQQGLADRLASVKLYGLPDDYLDKYVTRIRSVEPAQVRSMASKYIDAG
                     DSALVVVGDASKIAKDLEKLGKFTVEKPQ"
     gene            397..921
                     /locus_tag="VFQ79_10820"
     CDS             397..921
                     /locus_tag="VFQ79_10820"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0140198.1"
                     /translation="MKLLSSKERYRAKIFRITHDKAVDPDGFTIERDIVRHSGSAVMM
                     AVDDRRRILLVRQYRLPARATLWELPAGRLDPGEKPLDAAKRELREETGYTARRWEKL
                     LEFYPSPGFVGEKMTVFLARELTAGPSEQMDDERITMEWFTPSKLNRMVRRGEILDGK
                     TIAGFLAWRQWKRG"
     gene            complement(1133..1348)
                     /locus_tag="VFQ79_10825"
     CDS             complement(1133..1348)
                     /locus_tag="VFQ79_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU0140199.1"
                     /translation="MKQYAVIIERVPGSNYCASVPDLPGCVSTGDTPEEVRRNIREAI
                     EFHLEGMREDGDPIPEPTTQVEYAEVA"
     gene            1713..2606
                     /locus_tag="VFQ79_10830"
     CDS             1713..2606
                     /locus_tag="VFQ79_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140200.1"
                     /translation="MPNISPLPLWLGDGRIADELKDRFVFLDPVTNEVVVLTPSDNAA
                     GPAKQLRFELQNMALTVVSTTVTQRQDGLYAYEYNIRVAPGSRRPLRQWSLLLPGLDS
                     KLSADVSSLWQTETAQTDMVDRLASKHVPLKYLHFKVKPGYEMAAGGAALEVRIISKY
                     APGYVTAFSRSKPAKELPPAGMAELAKDVVDQIAKVLEPEFDSRMELVIGPRFDRDTP
                     MATVASSFDFAMQHFTVRGDMTDASEFAKSCTAAIRWYTESATESINVAPPALDCTTK
                     AQTQREKEIAHAMRLSLVRDR"
     gene            2638..3492
                     /locus_tag="VFQ79_10835"
     CDS             2638..3492
                     /locus_tag="VFQ79_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140201.1"
                     /translation="MPRMLMLALAALSGCGIVGAQTYNKFYTPGSGWDTYWAYCQGLN
                     TGQIVYNCPLMIETANYPGTNAHYHYNPPAYVSNMICVRREQQCSPYSGTRIYGNTSS
                     FGSVPFDLYPGQVGQAERVAATATETGKGNYLDYVVGYKDIYWNDHPEIWQQVGGSTT
                     NHGDNQYNHWMKSAPAYRLYDCTLEYLATFQQQKICTNDMSLPFGGVFDLNNNWYQPH
                     TFHRKGDSVDIPTTAANQCPSYKVPDANAPVFLNMCINKHQAMPVQGNCGGSCIESNH
                     IHFRWNVP"
     gene            3489..4460
                     /locus_tag="VFQ79_10840"
     CDS             3489..4460
                     /locus_tag="VFQ79_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140202.1"
                     /translation="MTYAAILIPLLPLAGALFGQPAYFESEPPAPVWQPSMETDARYH
                     GQHVFLSPDHTEIILRYSQPGETGPKRMVLRVRIKNRIAPEIKVDVSRGPGGGYVYRY
                     AVTNAASASDTIWTFGVVVPVELPQSPLRRESWIGPSIGVGLPSAVQILAPGHPAGRL
                     AIWFPHLAQGGRAISPGGTENGFILETSYRPGFTTSQLSDSADRLAEWPHTDEHWPDE
                     VASQLDGVLRWVLSGRSYVTIGPVFAPDETPEVVARRLKEALDLLEQGKVIDPGSPFI
                     SGVHTLLDRIQAVGIEGAGPIESSPSTRRERELADVLRLSLGIGALQ"
     gene            complement(4572..5387)
                     /locus_tag="VFQ79_10845"
     CDS             complement(4572..5387)
                     /locus_tag="VFQ79_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU0140203.1"
                     /translation="MLILGCGYTGRRAARTLLEKGFRVTATTRDPVKLVGLARAGARV
                     LRLEVLEPKTVAALEVPENALVIDSIPSVGNLDPTKVLVQKLGTRPAKVVYISTTGVY
                     GETQQVDENTKPAPRTDRERLRAQAEAAVEAGPWPSLILRSPAIYGPDRGVHISMKAG
                     TYRLPGSGSNFISRIHVEDLAALVVAGLLSDITGAWPVADHEPCTSEEAARYCSELFG
                     LPMPPRSDSDSLHESRRADRRVNGEAIRRLLGVVLRYPSYREGIRAAVETGWK"
     gene            5506..6675
                     /locus_tag="VFQ79_10850"
     CDS             5506..6675
                     /locus_tag="VFQ79_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140204.1"
                     /translation="MNRIKTAVIGAGFMGRVHTEGIRRLGNVDVVACCAINEEEATAF
                     GKAMGIERTTSDYKTILNDPSIQAVHVCTPNALHYPISRDALNAGKAVLCEKPLTLSV
                     KEAQELVDLAESKKLPNCVNHNLRYYPVVQQIRRMVEKGELGDILVVQGTYSQDWLLY
                     DTDFNWRIEAKDNGPLRAMGDIGSHWMDMIQHLTGLRITRLCADLQIFHKTRKKPKVA
                     IETFAGKTLKPEDYDEVPIDTDDYGAVLIHLGGRCRGAFTVSQVAAGCKNRFQVEVYG
                     TKGGAIWNQERPDELWLGERNSPSRLVLKDPALLDREAAAYADLPGGHSEGYDDTHKQ
                     VYRRFYRKVADASAPVEYPTFRDGLRGMQLLEKVLESAKKQAWVDTGLAAAAGGD"
     gene            6684..7430
                     /locus_tag="VFQ79_10855"
     CDS             6684..7430
                     /locus_tag="VFQ79_10855"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0140205.1"
                     /translation="MRPVMFVLLAAATLAAQQDQQPSKVDPGGPGKAPSDAEVLFDGT
                     DLTKWRSGDGGPAGCQVEEGDMVCRTGSGNVFSGDEFGSAQMHVEFNVPFMPSEKDQG
                     RGNSGFYIQGRYEIQILDSYDNPTYPTGQAGALYDDHAPLVNASRPPGEWQSYDIIFH
                     AFQCDPQGNILRPGTLTVFHNGVLIHDNVPIRKVHGACIQRGPIMLQDHAFPGAPITV
                     MRFRNIWVRPIDDGGASTIRGRTGIGRGGR"
     gene            7561..>8109
                     /locus_tag="VFQ79_10860"
     CDS             7561..>8109
                     /locus_tag="VFQ79_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140206.1"
                     /translation="MPDVIFEPLSFRNLTVKNRIFRSNISGRFDNYDGSGNQARVNWE
                     LKFARGGVGAIISSFVPVTIRGRIVPNYATIDRDERIPFWRELGRRVHEFDCKFIMQL
                     SHSGRQRDIPGIEYDKGLSATSNADPVHGFQCERMTTAQIRETVEAFAAGARRARDAG
                     LDGVELHGANGYLITQFLSSAIT"
BASE COUNT         1717 a         2394 c         2420 g         1578 t
ORIGIN      
        1 cggatcgctg gccgccgtca cgcaggtccg taatgaagtc gcacaaactg cactggcgga
       61 tctgctggcc atcatgaagg atttgggcaa caccccggca cccgccgccg agcttgcaac
      121 tgccaagaac ctcctgagcg ggactttcgt tctgagcgtc gagacccagc agggccttgc
      181 cgaccggctg gcctccgtga aactgtacgg actgccggac gactacctgg acaagtatgt
      241 gacgcgcata cggagcgttg agccggcgca ggtgcgctcg atggcgtcga aatatatcga
      301 tgcgggggac tccgcgctgg tggtggtcgg cgacgcctcg aaaatagcaa aggatctcga
      361 aaaactcggt aaattcacgg tggaaaaacc gcaataatga agcttttgtc ttccaaagag
      421 agataccgcg cgaaaatttt ccgaataaca cacgacaaag cggtggaccc ggacggcttc
      481 acgattgagc gcgacattgt ccggcacagc ggctcggcgg tcatgatggc ggtcgacgat
      541 aggcgccgga ttctgctggt gcggcagtac cgcttgcccg cccgtgccac tctgtgggaa
      601 ttgccggccg gacggcttga tcccggcgag aaaccactgg acgccgcgaa acgggaactg
      661 cgcgaagaga ccggctatac cgccaggcgc tgggagaagc tgctggagtt ttatccaagt
      721 cccggcttcg tgggggagaa gatgaccgta tttctggcgc gagagttgac cgccggacca
      781 tccgaacaga tggatgacga gcgcatcacg atggagtggt ttacacccag caagctcaac
      841 cgcatggtcc gccgtggcga aatcctcgat ggcaagacca tcgcgggttt tctggcctgg
      901 cgtcagtgga agaggggata acggcgatcc gaattatcaa cgggtgatgc gctttccccg
      961 cagttccatg cacttcagac agcggcatcg cggcgtatcg ggattatgct gtttccagta
     1021 cattccaccc gaataggatt ctcgcttcaa ggcgttgcgc cgggctcgtt ccgttttgag
     1081 aacagcatct ggaatcgttg ccgggtcgat gtcattcgtc cagcgtttca aatcaagcga
     1141 cctcggcgta ttcgacctga gtggtgggtt ccgggattgg atccccatcc tcccgcatgc
     1201 cctccaggtg aaactcgatg gcttcccgga tattgcgtcg tacttcctcc ggcgtatcac
     1261 cggtggagac gcaaccaggc aggtcgggta ccgaggcgca gtagttgctt cccggcacgc
     1321 gctcgatgat aacggcatac tgtttcatcg tttcttcctc tcaatttggg cttgagtcaa
     1381 gatactgctg agcagcttag gcgtcaggtc atcgccaggc tttccagaaa tggtcaccct
     1441 cccgcgtttc accgggaagt tagacggtgc atccgcacgt agactgaaac cgttctctag
     1501 gcccctctat cactcatgga gatacctgac acagtagcag aaaattggcg aaatatagtt
     1561 ctaaatatct tgaaaaagat ctaaattcct ataatctaga tggcgtgaac cgaaattcgt
     1621 tgggattgga ggacaaaccg atgttgagaa tagccatttt ggtgatattt ttaactgcct
     1681 ttggcttatt tgcactgtca gcgcaggatg tcgtgcctaa catcagccct ttgcctctct
     1741 ggcttgggga cggccgcatc gccgatgaat taaaggatcg cttcgtattc ctggatcccg
     1801 tgactaatga ggtggtcgtg ctaacgccgt ccgataacgc agccggcccg gcaaagcagc
     1861 tgaggttcga acttcagaac atggccttga ccgtggtgag cactacagtg acgcagcggc
     1921 aggatgggtt atacgcgtat gaatacaata tccgggtggc gccgggcagc cggcggcctc
     1981 ttcggcagtg gtctttgctg cttcccgggc tggactcgaa attgtcggcc gatgtctcat
     2041 ccctctggca gacggagacg gcacagacgg atatggtaga ccgtctggcg tccaaacatg
     2101 tgccgttgaa gtaccttcac tttaaggtga aacccggcta tgaaatggcg gctggcgggg
     2161 cagcccttga agtccggatt atctcgaagt atgcgccagg atatgtgacg gcgttctcac
     2221 gaagcaaacc ggctaaggaa ctccctccgg ccggaatggc cgaactggcg aaggacgttg
     2281 tggaccagat cgcgaaagta ctcgagccgg aattcgacag ccgcatggag ttggtgattg
     2341 ggcctcgatt tgaccgcgac acgcccatgg cgacagtcgc gtccagcttc gattttgcga
     2401 tgcagcattt cacggtgcgc ggtgacatga ctgacgcttc cgagttcgct aaatcgtgca
     2461 ccgccgcgat acggtggtat accgaatcgg cgaccgaatc gatcaacgtt gcaccgcccg
     2521 ctctggattg caccaccaag gctcagacac agcgcgagaa ggaaatcgcc cacgcgatgc
     2581 gtctgagcct ggtccgtgac cgctgatgtt tgacattggc taagaaagga gacacaaatg
     2641 ccacgaatgc tcatgcttgc actcgccgct ttgtctggct gcggcatagt tggggcgcag
     2701 acgtacaaca agttctacac gccgggatcc ggttgggaca catactgggc gtactgccag
     2761 gggctgaaca cgggccagat cgtctacaac tgccccctga tgatcgaaac ggcaaattac
     2821 ccgggaacca acgcgcacta ccactacaac cctcccgcgt acgtcagcaa catgatttgc
     2881 gtgcgccgtg agcagcagtg ctcgccgtat agcggaactc gaatctatgg gaacacgagc
     2941 agcttcggga gcgtgccgtt tgatctgtat cccgggcagg tgggccaggc ggagcgggtc
     3001 gctgccaccg ccacggaaac cggtaagggc aactatctgg attacgttgt gggctacaag
     3061 gacatctact ggaatgacca cccggagatc tggcagcaag tcggcggaag tacgacgaac
     3121 cacggcgaca atcaatacaa tcactggatg aagagcgcgc cggcgtatcg gctctacgac
     3181 tgcacactgg agtatctcgc cactttccaa cagcagaaga tctgcacgaa cgacatgtcc
     3241 ctgccgttcg gaggcgtgtt cgaccttaac aacaactggt atcaaccaca cacatttcac
     3301 cggaagggag attccgtcga cattcccact accgcagcga accagtgccc gagctacaag
     3361 gtgccggatg cgaatgcgcc agtgttcctg aatatgtgca ttaacaaaca ccaggcgatg
     3421 ccggtgcaag ggaactgtgg cgggtcttgc atcgaatcca accacattca cttccggtgg
     3481 aatgtgccat gacatacgcg gcgattctga ttcccttgtt gccgctggcc ggcgcgctgt
     3541 tcggccagcc ggcatacttt gagtccgaac cgcccgcgcc cgtgtggcag ccctcgatgg
     3601 agaccgatgc gcgctaccac gggcagcacg ttttcttatc gccggaccac acggagatta
     3661 tcctccggta ctcgcagcca ggcgaaaccg gccccaagcg gatggtcctc cgggtgcgca
     3721 tcaaaaaccg gatcgctccc gaaatcaagg tggacgtatc ccgggggccg gggggtggct
     3781 acgtctatcg ttacgcggtg accaatgccg cgtccgcgag cgacaccatc tggactttcg
     3841 gagtcgtggt gccagttgaa ctgccgcagt ccccgctgcg cagggaaagc tggatcgggc
     3901 cgtcgatcgg tgtaggacta ccctcggcgg tccagatcct ggcccccggc catccggcgg
     3961 gacggctcgc catctggttc ccccacttgg cccaaggcgg gcgggcgatt tccccgggcg
     4021 gaacggagaa cggattcatc ctcgaaacga gctaccgtcc cggttttacg acgtcgcaac
     4081 tgtccgactc agcggatcgg ctagcggaat ggccgcatac ggatgagcac tggccggatg
     4141 aggttgcgag ccaactggac ggcgtgctgc gttgggtcct gtccggaagg agctacgtga
     4201 cgattggtcc tgtcttcgcg ccggacgaga cgcccgaagt tgtagcccgg cgtctaaagg
     4261 aggctcttga cttgctggag cagggaaagg tcatcgatcc cggttcaccg ttcatcagcg
     4321 gcgtccacac ccttctcgac aggatccagg cagtggggat agagggcgcc ggtccgatag
     4381 agagcagtcc atctaccagg cgcgagcgcg aactggcgga cgtcttgcgg ctcagcctgg
     4441 ggatcggcgc gctacagtga atagtcgccg ccgtgctgca cgcctctatc aactcaggcg
     4501 cgacagccaa tccggcatca gaacggcaag gaagtctcaa atctcaacga gtccggcgcg
     4561 gggcatcgta gctacttcca tcccgtttcc accgctgcac ggattccttc tcgataggaa
     4621 gggtatcgca acaccacacc aagcagccgc cggatagctt ctccgtttac gcggcgatcc
     4681 gcgcggcgcg actcgtgcag agaatccgaa tcggaccggg gcggcatcgg caatccaaac
     4741 agctctgaac aatagcgggc ggcttcttcc gatgtgcaag gctcgtggtc cgccaccggc
     4801 catgctccgg ttatgtccga caggagcccg gctacgacga gagccgcaag atcttccacg
     4861 tgaatgcggc tgatgaaatt tgagccgcta cctggtaggc gatacgtccc ggccttcatc
     4921 gagatatgca ctccacggtc cggtccgtaa atggcaggcg agcgaagaat cagagatggc
     4981 caggggccgg cttccaccgc ggcttcggct tgtgcacgta accgctcgcg atccgtgcgc
     5041 ggcgccggtt tggtattttc atccacctgc tgcgtttcgc cgtaaacgcc cgtcgtcgag
     5101 atatagacca cttttgccgg ccttgtcccg agtttttgaa caagcacctt cgtggggtcc
     5161 aggttgccta ccgacggaat agaatcgatc accaaagcat tctccggcac ctcaagcgcc
     5221 gcgacggttt tgggctccag cacctccaga cgcaggaccc gggcgccggc gcgggcgagg
     5281 cccacaagct tcaccggatc gcgcgtggtc gcggtgacac gaaacccttt ctccagcagg
     5341 gtccgcgccg cgcgtcttcc cgtgtagccg caacccagaa tgagcacttc gttcaaatgt
     5401 tccagacctc cagtatcgga ccagttgcgc taaagtgtgg ggaggtattc gacggagccg
     5461 cgctcgtcag tgagcgctga tataccgaaa ggatcttgcc agattatgaa ccggattaag
     5521 actgctgtca taggtgctgg atttatgggg cgggtgcaca ccgagggcat ccgccgtttg
     5581 ggcaacgtcg atgtcgttgc ctgctgcgcg atcaatgagg aagaggccac cgcatttggg
     5641 aaggccatgg gaatcgaacg tactacttcg gactacaaaa cgattctcaa cgacccttcg
     5701 attcaggcgg tacatgtctg cacgcccaat gcgctgcact atcctatttc gcgtgacgcc
     5761 ctcaatgcag gcaaggccgt tctttgtgaa aagcccctga cgctttccgt caaggaggca
     5821 caggaactgg tggacctggc cgagagcaag aaacttccga actgcgtaaa ccacaacctc
     5881 cgctactacc cggtggtgca gcagattcgc cgcatggttg agaagggcga gctgggtgac
     5941 atccttgtcg tgcagggcac ctattcgcag gactggctgt tgtacgacac ggacttcaac
     6001 tggcgcattg aagcgaaaga caatggtccg ctgcgagcca tgggcgatat cggttcgcac
     6061 tggatggaca tgattcagca cctcaccggg ctgcgcatca cccggctgtg cgcggacctc
     6121 cagatctttc acaagacgcg caagaagccc aaggtggcta ttgaaacctt tgccggtaaa
     6181 acgctgaagc cggaagatta cgacgaagtg ccgatcgata ccgacgacta cggcgccgta
     6241 ctcatccatc tgggcggccg ttgccgcggc gcgttcaccg taagccaggt ggcggcgggc
     6301 tgcaagaacc gcttccaagt ggaagtgtat ggcaccaagg gcggagccat ctggaatcag
     6361 gaacggccgg acgagctatg gctgggcgaa cgaaattccc cgagccggct ggttctgaaa
     6421 gacccggcgt tgctggaccg cgaagccgcc gcgtatgcag accttcccgg aggccacagc
     6481 gaaggatacg acgatacgca caaacaggtt taccggcggt tctatcgcaa agttgccgat
     6541 gcttcggcgc cggtcgagta tccgacattc cgcgacggtc ttcgcggaat gcagttgctg
     6601 gagaaagtcc tcgagagcgc caaaaagcag gcttgggtag acacaggcct ggcggctgcc
     6661 gcgggaggag attaatccgt gggatgcgac ctgtgatgtt cgtcctgctg gccgctgcga
     6721 ctctggcggc ccagcaggac cagcaaccca gcaaagtgga tccgggtgga ccgggcaaag
     6781 cgccgtccga cgcggaggtt ctgtttgacg gcacggatct gacgaaatgg agatccggcg
     6841 atggcgggcc tgccggctgc caggtcgaag agggcgatat ggtgtgccga accgggtcgg
     6901 gcaatgtgtt ctctggcgat gaattcggaa gcgcccagat gcacgtggaa ttcaacgtgc
     6961 cgttcatgcc ttcggaaaag gaccaggggc gcgggaacag cgggttctac atccagggac
     7021 gctatgagat tcagattctc gattcctacg acaacccgac ctatcccacc ggacaggccg
     7081 gggcgctata cgacgatcac gccccgctgg tgaatgcctc gcggccgccg ggcgaatggc
     7141 aaagctacga cattatcttt cacgcgttcc agtgcgatcc gcaaggcaac atactgcgcc
     7201 ccgggaccct gaccgtgttt cacaacggag tcctcattca cgacaacgtg cccatccgga
     7261 aagttcatgg cgcctgcatc cagcgcggac ccattatgct ccaggatcat gcctttcccg
     7321 gcgcgcctat aaccgtcatg cggttccgca acatctgggt ccgccccatt gacgatggcg
     7381 gcgcaagcac cattcgcggg cggaccggta tcgggcgggg cgggcgctga gccgggccgc
     7441 cttcagtcgg gcacttcttc cagtttccac aggccggtac ccacccagta cgcgaatact
     7501 tcctttacac cttcccttgc gacataatac tcacaactaa cagtactgga gatctctggg
     7561 atgcctgacg tcattttcga accgctcagt ttccgcaatc ttacagtgaa gaacagaatc
     7621 ttccggtcca acatctccgg ccgcttcgat aactacgacg gctcggggaa ccaggcgcgc
     7681 gttaactggg agctgaagtt cgcgcgcggc ggcgttggcg ccatcatctc ctcgttcgtg
     7741 ccggttacca tccgcgggcg cattgtgccc aactatgcga cgatcgaccg cgacgagcgc
     7801 attcctttct ggcgtgagct cggccggcgc gtccacgaat tcgactgcaa gttcattatg
     7861 caactcagcc attccgggcg gcagcgcgac atcccaggca tcgagtacga caagggactg
     7921 agcgccacct cgaacgccga cccggtccat ggttttcagt gcgagcggat gaccacggcc
     7981 cagattcggg aaaccgtcga ggccttcgcc gccggagccc ggcgcgcgcg cgatgcgggg
     8041 ctggatggcg tggaactgca cggcgccaac ggctacctga tcacccaatt cctcagctcc
     8101 gctatcacc
//
LOCUS       DASTMK010000251         7515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_203195, whole genome shotgun sequence.
ACCESSION   DASTMK010000251 DASTMK010000000
VERSION     DASTMK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203195"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFQ79_10865"
     CDS             complement(<1..717)
                     /locus_tag="VFQ79_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0140207.1"
                     /translation="MLPAGLYRTLLCCYPAAFRHEYSAEMIAAFTEQVREARRQGGWM
                     AEVPIWLRALFDVLSTAPREHFHVIQQDLRYAIRTLASRPGFTAVAVLSLAFGIGANT
                     AIFSLINSILLSALPVRDPQSLVILTNPASNGIMIGGKSGEREELTYSEFLQLRELSP
                     VFASLMACQIEPERIPVRVDGSEPEEIVVQLVSSEYFSTLGVPALLGRTLSPDDDPAA
                     PNAVISYDYWQRRFGGRADVL"
     gene            complement(721..1083)
                     /locus_tag="VFQ79_10870"
     CDS             complement(721..1083)
                     /locus_tag="VFQ79_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0140208.1"
                     /translation="MSPDVSDPDSLLPLPTAVFHILVALADRDRHGYSIMQDVAARTG
                     GKVRLSPGTLYSSIHRMLEQGLVEELRESPDPESQDERRRYYRLTQWGRRVAMAEARR
                     LTDMLSQARETGLIPKKL"
     gene            1264..2103
                     /locus_tag="VFQ79_10875"
     CDS             1264..2103
                     /locus_tag="VFQ79_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0140209.1"
                     /translation="MTRLAKMGALVLLLALSASGGSIFLTGHDPDFHALLGGNLTGAR
                     NINTVAINFTMDAAFNPYVSAGANKFLFVESKISPPGGHTRGVNGIVASGYTAGTDFE
                     HHDASTLGAELDLLGTKYGGIVVASDFGGVLTAAELNILNSRSADIISFLNQGGGIYA
                     MANSNSQARLTSGATPYGYLPFVVTAPQLNQNEFGFTVTAFGASLGLANTDVNGNASH
                     NVFAGTFGMQIVDVDAGGRILSLATRSMIDEEGVVPEPSSALLFLAGLGTIVFLGRRK
                     LQR"
     gene            2290..2493
                     /locus_tag="VFQ79_10880"
     CDS             2290..2493
                     /locus_tag="VFQ79_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0140210.1"
                     /translation="MKEAGTVKWFNAAKGYGFIQRESGEDIFVHFSAIEGAGYRSLDE
                     GARVSFLVKRGPKGLQAEQVELA"
     gene            2650..3408
                     /gene="modA"
                     /locus_tag="VFQ79_10885"
     CDS             2650..3408
                     /gene="modA"
                     /locus_tag="VFQ79_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0140211.1"
                     /translation="MLVSVLCLAAGCGREQKPRELTVAAAANLNVVLEEIGKAFTAQT
                     RSRVLFSYASTAQLTQQIKNGAPFDLFLAADTEHVDQLIEQGKVLPDSRAIFARGKLV
                     LWVPPESPADIRRLEDLLKPGVRMIAVASPGSAPYGRAAVETLKAVGLWDRVERKIVY
                     ANNVNLARQYAASGNAEAAFTAYSLVLHSGGHIVDVDPASHHPLEQALGIVAGSPNQK
                     PAREFVRFLQNGEGAGLLEKYGYDVPGHRIKPRR"
     gene            complement(3392..4648)
                     /locus_tag="VFQ79_10890"
     CDS             complement(3392..4648)
                     /locus_tag="VFQ79_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0140212.1"
                     /translation="MNSRQRVRAALSHQCPDRLPIDMGSTSVTGMHVSCVAALREYYG
                     LDRHPVKVIETTQMLGEIEEDLKQVLGIDVEGIPPRKASFGIPMNATKPWRMYDGLEV
                     LMPAGFEFTVDANGDTLVYPQGDRTAAPSARMPAGGYFFDVIIRQQPIDEDRLDPADN
                     VEEFSLLSPEDVAYFEAEARRASETGRAVVGKFSSGLGDIAAVPGMALKKPKGIRDIT
                     EWYMSIKSRPDYIREVLWRQSEAILENLRRVHGACAGTVDVAYICGTDFGTQKSTFCS
                     PETFKNLWFPCYKRINDWIHQHTSWKTFKHCCGSVVKLLDLFIDAGFDILNPVQISAA
                     GMDPEYLKSTWGDRLVFWGGGVDTQHVLAFGTPAEVREQVLRNCEVFARGGGYVFNTV
                     HNVQARTPVQNIVSMFQAVREFSGAA"
     gene            complement(4679..5452)
                     /locus_tag="VFQ79_10895"
     CDS             complement(4679..5452)
                     /locus_tag="VFQ79_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEU0140213.1"
                     /translation="MRTILSQPGACDNLEGCRRSSLRNRNMSLEWKTEDEMFAIARRE
                     LFSAVLGDIMDKMGLRRQFLPPSIQPLRTDMVVAGRAMTVLEADFFEEAGAGGNNPLS
                     AKPFGLMLEALDDLKPNEVYVCTGASPSYALWGELMSIRATRCGAAGAVLDGYSRDTN
                     GILELGFATFAHGRYAQDQGPRGKVIDFRIPIEMGGVRIVPGDIVYGDLDGVCVVPRA
                     AEEEVFARALEKARTEKAVKKALEAGMSAREAFDRFGVL"
     gene            5453..6988
                     /locus_tag="VFQ79_10900"
     CDS             5453..6988
                     /locus_tag="VFQ79_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0140214.1"
                     /translation="MNRRRALGSLWAGAVAATAAGQEGTRRVAPLKKVTGTKARNVVF
                     ILADDHRYDALGFVKGQPFLETPHMDSLARDGAHLKNAFVTTALCSPSRASILTGRYA
                     HGHRIVDNNTPIPAGTTFFPQYLQQAGYSTAFIGKWHMGHEDDAPQPGFDFWASFRGQ
                     GSYLPSKNGLNINGKRVPQKGYITDELTDYAIDWLQNRDRSKPYFLYLSHKAVHGDFI
                     PPERYKGRYTDAKFVPPPTMAASGEMAQHRPMWVQNQRNSWHGVEYAYHSDVDISVYY
                     KRYAETLLAVDDSVGRVLDELRKRGELDSTLVIYMGDNGFAFGEHGLIDKRTAYEESM
                     RVPMLACCPELFSGGRVVNEVVANIDVMPTVLAAAGLESPAGLDGQNMLPVLQGKGER
                     WRDELLYEYYWERNYPQTPTIHALRGNRYKFIRCHGVWDIDELYDLQEDPLEARNLIF
                     SEQHAGIAKQMRERLFEILEQTGGMSIPLYPDRGGTSNLRNPKGSKTAGFPPELVRPP
                     VRR"
     gene            complement(6989..>7515)
                     /locus_tag="VFQ79_10905"
     CDS             complement(6989..>7515)
                     /locus_tag="VFQ79_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140215.1"
                     /translation="LAYETPIIRQRSNWMQQVFGNWNLSAVLLLKNGTPFTVLAGSDA
                     PGYGNVDANGGDRPNLLDSSVLGRTIGDPDTSRERLPRSAFQYMEPTDERGNLGQNTF
                     RKGGIRNVNAAVSKDWLVTGEKRLTFRAESINLLNTPQFAAPGNELALPNFGQITNTL
                     NDGRTLRFLLQFAF"
BASE COUNT         1572 a         2212 c         2250 g         1481 t
ORIGIN      
        1 aagaacatcg gcacgaccgc cgaagcgtcg ctgccaatag tcgtagctga tcacggcgtt
       61 cggcgcggcg ggatcatcat ccgggctgag tgtgcggccc agcagagccg gcacacccaa
      121 agtgctgaag tattcggacg agaccagttg gacgacgatc tcttccggct cggacccatc
      181 cactcttacg gggatgcgtt cgggctcgat ctgacaagcc atcaagcttg caaataccgg
      241 ggacagctct cgcagttgga ggaactcgga ataggtcaac tcctcccgct cgccactctt
      301 ccctccaatc atgatcccgt tcgaggcggg attcgtcaga atgacgagcg actgcggatc
      361 gcgtaccggc agggcgctca acagaatgct attgatcagg ctgaaaatcg ccgtgttcgc
      421 cccgattcca aatgcaagcg aaaggacagc taccgccgta aatcccggcc gcgaagcaag
      481 ggtgcggatt gcataacgca ggtcttgttg aatcacgtga aaatgctccc gtggtgcggt
      541 gcttaaaaca tcgaagagcg cgcggagcca gatcgggacc tctgccatcc atccgccctg
      601 ccgccgcgct tcccgcacct gttcggtgaa cgccgcgatc atctccgcgc tgtactcgtg
      661 gcggaaagcc gccggatagc aacagagcag cgtccggtaa aggccggcgg gtagcatcgc
      721 ttacagcttc tttggaatca ggccggtttc gcgagcctgg ctcaacatgt ccgtcagccg
      781 cctggcctcc gccatcgcca cgcgccggcc ccattgcgtc aggcggtaat agcgccggcg
      841 ctcatcctgg ctttccggat ccgggctctc acgcagttct tccaccaggc cctgctccag
      901 catccggtgg atggagctgt acagcgtgcc ggggctcaga cgaactttgc cgccagttcg
      961 agccgcgacg tcctgcatga tggagtatcc gtggcggtcg cgatcggcca gcgccaccag
     1021 aatatgaaac accgccgttg gcagcggcag aagggaatcg ggatcggaga cgtccgggga
     1081 cataatctat gtcgtgtatg agtataattg aaaccgttat agtatgcaag gcggaatagg
     1141 taaaatccaa tagccaggaa ccatttatcc cttgtaaaaa agggttgtaa tttcgtggaa
     1201 ctgtgctagc attgattcgc ttttccagag acaggccaga ctgggtgcta ttgggggtgc
     1261 gcaatgactc gcttagcaaa gatgggtgcg ctggttctgc tgttggcgct cagcgcgtcc
     1321 ggtggatcga tttttctcac cggccacgat ccggatttcc atgccttgtt ggggggcaat
     1381 ctgaccggcg caaggaatat caacaccgtt gcgatcaact tcaccatgga cgcagcgttc
     1441 aacccctacg tcagcgccgg ggcaaacaag tttctttttg ttgaatcgaa gatctcccca
     1501 cccggggggc acacgcgcgg cgttaacggc atcgtggcca gcggctacac ggcgggaacc
     1561 gatttcgaac atcacgacgc cagtacgctt ggagcggagt tggacctgct tggaacgaag
     1621 tatggcggca ttgtggtggc gtccgatttc ggtggtgtac tgacggccgc ggagttgaat
     1681 attctgaata gccggtcggc cgacattatc tcgttcctga accagggcgg cggcatctat
     1741 gccatggcaa acagcaattc gcaggcgcgg ttgacatcgg gcgccacgcc ctatggctat
     1801 ctcccgttcg tggtcacggc gccgcaattg aaccagaacg agttcggttt taccgtcacg
     1861 gcattcggag cttcgctcgg actggccaac accgacgtaa acgggaatgc gtcgcataac
     1921 gtgttcgcgg gcacgtttgg aatgcagatc gtggatgtgg atgcaggtgg acggatcctg
     1981 agcctggcaa cccgcagtat gatcgatgaa gaaggtgtgg ttccggaacc ctcctcagcg
     2041 ctgttatttc tggccggatt gggcacgatt gtgtttctcg gcagacgcaa actgcagaga
     2101 taaaaccgga caacaaaaaa cattcattcc ctattgcgat tgggcttggc tcctggtaca
     2161 ctgtagttgc ttttgaatac ttgtaagtcg cttgtgaagc ttcctgccca aacccccaaa
     2221 aaaatggtgc gtcaccgcta ggattgtcct caaacgatca tcagggattt caatgcacga
     2281 aaggaaaaca tgaaagaagc aggcacggta aagtggttta acgccgcaaa aggctatggt
     2341 tttattcagc gggagagcgg cgaagacatc ttcgtgcatt tctcggcgat cgaaggggca
     2401 gggtaccgct ctctcgatga gggcgcgcgc gtatcgttct tagtgaagcg gggacccaag
     2461 ggtcttcagg ccgaacaagt agaactggcg taggctttca cagggctggc ggcacaacaa
     2521 gcgccagccc tttcaactct ccctctttcc gtcccttaac gggtgcgcgg aaccggtgtt
     2581 ctgacgcaca ctggtatttt gtctccaggt atccaactcc gccatcgaac ctttcaaaca
     2641 tcagccgctg tgctggtcag tgtattgtgc ctggccgccg gatgcggacg tgagcagaag
     2701 cctcgggaac tgaccgttgc cgccgcggcg aacctcaatg tggtcctcga agaaatcggg
     2761 aaagccttca cggcacagac ccggtcacgc gtgctgttca gctacgcttc gacggcacaa
     2821 ctgacgcagc agattaagaa cggagcgccg tttgacctct tcctggcagc cgacaccgag
     2881 cacgtcgatc aattgattga acagggaaaa gtgcttccgg acagccgcgc tattttcgct
     2941 cgcggcaagc tggtgctgtg ggtcccaccg gagagcccgg cggacattcg acggctggag
     3001 gaccttttga aacccggcgt ccggatgatt gccgtcgcaa gtcccggcag cgcgccgtac
     3061 ggcagagcgg cggtggagac gctaaaagcg gtggggttgt gggaccgcgt agaacgcaag
     3121 atcgtctatg ccaacaacgt gaacctcgcg cgccagtacg cggcatccgg caacgccgaa
     3181 gcggctttca cggcatactc gctcgtgctc cactccggcg ggcatattgt cgacgtcgat
     3241 ccggcgagcc accatccgct ggaacaggcg ctcggcattg tggcaggatc gccgaaccag
     3301 aagcccgcgc gcgagttcgt caggttctta cagaacggag aaggcgccgg cctccttgag
     3361 aagtacggtt acgacgtccc ggggcaccgc atcaagccgc gccgctgaat tcccgaacgg
     3421 cctgaaacat ggaaacgatg ttttgtaccg gcgtccgcgc ctggacattg tgcaccgtgt
     3481 tgaagacgta acctccgcca cgcgcaaaga cttcgcaatt ccggagcacc tgctcgcgga
     3541 cttccgccgg tgtaccgaac gcgagcacat gctgcgtatc gacgccgcct ccccagaaca
     3601 ccagtctgtc gccccaggtg gatttcaagt attccggatc catacccgcc gctgaaatct
     3661 gcaccggatt gagaatatcg aagcccgcat cgatgaaaag gtcgaggagt ttcacgaccg
     3721 aaccgcagca gtgtttgaag gtcttccagg acgtatgctg gtgaatccag tcgttgatgc
     3781 gcttatagca cggaaaccac aaattcttga acgtctcagg ggaacagaag gtggacttct
     3841 gcgttccaaa atccgtgccg caaatatagg caacgtcgac ggttccggca caagccccat
     3901 gcacccggcg gagattttcg agaatcgcct ccgactggcg ccatagcact tcgcggatat
     3961 agtccggcct gcttttaatc gacatgtacc attcggtaat gtcgcgaatc cctttgggtt
     4021 ttttaagcgc catacccggc acggcggcga tatcgcccag gccggaactg aacttgccga
     4081 cgaccgcccg gcctgtttcc gacgcgcggc gtgcctccgc ctcaaagtaa gcgacatctt
     4141 ccgggctcaa cagcgaaaac tcctcgacat tatccgcggg atcgagccgg tcttcgtcga
     4201 taggctgctg gcggatgatc acatcgaaga agtagccgcc ggcgggcatg cgcgcgctgg
     4261 gcgcggccgt acggtctcct tgcgggtaga caagggtgtc gccgttcgca tcgacggtga
     4321 actcgaagcc ggccggcatc agcacttcga gaccgtcata catgcgccac ggttttgtgg
     4381 cgttcatcgg gattccaaag cttgccttgc gaggcggaat tccttcgaca tcgatgccga
     4441 gcacctgctt gaggtcctct tcgatttcgc cgagcatctg cgtggtctcg atcaccttca
     4501 ccggatgccg gtctagaccg taatattccc gcagcgccgc gacgcaactg acgtgcatac
     4561 ccgtgacgct ggtgctcccc atatcgatcg ggagccgatc aggacactgg tgagatagag
     4621 cggcgcgaac tcgttgcctg ctgttcatgt tctttccttc ccgccggttt gccggacgct
     4681 acaacactcc gaaccggtcg aacgcttcgc gggcggacat gccggcctcg agcgccttct
     4741 tcaccgcctt ctcggttcgc gctttttcaa gcgcgcgcgc gaacacttct tcctccgcgg
     4801 ctcgcggcac aacgcagacg ccatccaggt cgccatagac aatgtcgccc ggcacaatgc
     4861 ggacgcctcc catttcaatg gggatccgga aatcgatcac tttgccgcgc gggccctgat
     4921 cctgggcata gcgcccgtgg gcgaaggtgg cgaagccgag ctccaggatg ccgtttgtgt
     4981 cgcgggaata tccgtcgagg acggcgccgg ccgcaccgca gcgcgtggcg cgtatactca
     5041 tcagctcgcc ccagagcgca taggagggcg acgctcccgt gcacacataa acctcattgg
     5101 gcttcagatc gtcgagcgct tcgagcatca gcccgaaggg ttttgccgag aggggattat
     5161 tccctcccgc gccggcctcc tcaaagaaat cggcctcgag tacggtcatc gcgcgccccg
     5221 cgacaaccat gtccgtccgc aggggttgaa tcgacggcgg cagaaactgc cggcgcagac
     5281 ccattttgtc cataatgtcg ccgagcaccg cggagaataa ctcgcggcgc gcgatggcga
     5341 acatctcatc ttcggtcttc cattccaggc tcatgttccg gttccgaagg gaactcctcc
     5401 ggcagccttc caggttatcg cacgcgcccg gttgggagag aatggtacgc atatgaacag
     5461 aaggcgagca ctaggaagtc tgtgggcagg cgcggtggcg gcgacggcgg ccggccagga
     5521 gggcacccgg cgggtagcgc cattgaaaaa agtgaccggc accaaggcgc gaaacgtggt
     5581 cttcattctg gccgacgatc atcgctacga tgcgctcggg tttgtgaaag gccagccgtt
     5641 tctcgaaacg ccgcatatgg attcgcttgc cagggacggc gcgcatctga agaacgcctt
     5701 cgtcaccacg gcattgtgca gcccgagccg ggccagtatt ctcaccggcc gatacgccca
     5761 cggacacagg attgtggaca acaacacccc gatcccggcg ggcacgacgt tttttccgca
     5821 gtatctacag caagccggat actccaccgc gtttatcggc aagtggcaca tgggccacga
     5881 ggacgacgct cctcaacccg gtttcgattt ctgggcgagc tttcgcggac agggctctta
     5941 ccttccatcg aagaacgggt tgaacataaa cggcaagcgt gtgccgcaga agggctacat
     6001 tacggacgag ctgaccgatt acgccataga ctggctgcaa aaccgggacc gctcgaagcc
     6061 gtattttctc tacctgtcgc acaaggcggt acacggggat ttcattcctc ccgagcgcta
     6121 caagggacgg tacacggatg cgaagttcgt tccgccgccg accatggccg ccagcggcga
     6181 gatggcgcag caccggccga tgtgggtaca gaaccagagg aacagttggc acggtgtcga
     6241 gtacgcttac cactccgacg tggacatctc ggtttactac aagcgatacg cggagacgct
     6301 gcttgcggtg gacgacagtg tcggcagggt gctcgatgaa ttgcggaagc gtggagaact
     6361 ggattccacg ctggtcattt acatgggcga caacggcttc gcgtttggtg aacacggact
     6421 catcgacaag agaaccgcgt atgaagaatc gatgcgtgtg cccatgctgg cgtgctgccc
     6481 ggaacttttc tccggtgggc gggtggtgaa cgaagtggtg gcgaacatcg acgtgatgcc
     6541 gactgtgctg gccgccgccg ggctggaatc tccggcggga ctcgacgggc agaacatgct
     6601 gccagtactt cagggcaagg gagagagatg gcgcgacgag ctgctgtacg aatattattg
     6661 ggagcgcaac taccctcaga cacccaccat tcacgcgctg cgcggaaacc ggtacaagtt
     6721 catccggtgt cacggtgtct gggacatcga tgagctgtac gatttgcagg aagatccgct
     6781 cgaagcgcgg aacctgatct tcagcgagca gcatgccggc atcgcgaaac agatgcggga
     6841 gcgtttgttt gaaattttgg aacagacagg cggtatgagt attccgctct acccggaccg
     6901 tggcggaacc agcaacctgc gaaatccgaa gggctcgaaa accgcggggt ttccgccgga
     6961 gctggtgcgc ccgccggtcc ggcgctgatc agaacgcgaa ctggagcaga aaccgcaatg
     7021 tgcggccgtc attcagcgtg ttggtgatct ggccgaaatt cgggagagcg agctcattgc
     7081 ccggcgcggc gaactgcggg gtattaagca ggttgatgga ctccgcgcga aacgtcaggc
     7141 gtttttcgcc ggtcaccagc cagtccttgg agacggcggc attcacgttg cggatgccgc
     7201 ctttccggaa cgtgttctgc ccgagattgc cccgctcatc ggtcggttcc atgtattgaa
     7261 atgcggagcg cggaaggcgc tcacgcgacg tgtccggatc gccgatggtg cggcccagca
     7321 ccgatgaatc gagaagattc ggacggtctc ctccgttggc gtccacgttg ccatagcccg
     7381 gcgcatccga acccgcgagc acggtaaacg gtgtgccgtt cttcaacagc agaaccgccg
     7441 acaggttcca gtttccgaac acctgctgca tccaattgga tctctggcgt atgatcgggg
     7501 tctcataagc aagcc
//
LOCUS       DASTMK010000252         4348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_331865, whole genome shotgun sequence.
ACCESSION   DASTMK010000252 DASTMK010000000
VERSION     DASTMK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331865"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1129
                     /locus_tag="VFQ79_10910"
     CDS             245..1129
                     /locus_tag="VFQ79_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0140216.1"
                     /translation="MLKLERILCPVDFSEFSAKAYDYAHSLARHYGAKLFVLHVTEPI
                     ISIYRGYMSAAMVDEIYTRQTADTQEKVRKLSERHQGGQVESDVVVQLGIVSDSILTL
                     AETQEIDLIVMGTHGRRGIDRLAMGSNTERVLRKALCPVLAVREPVRDFVSSASAVEP
                     VQLRKIVCCTDFSDNSPRALEYAFSLAAQYEAEVTLLHVLEDSGAGPRLEEQKEQAFK
                     RLEEVIPSEAQNWATVIPLVRAGKPYEEIMEHAAQTQADLIVMGVRGRNALDVALFGS
                     TTHRVIQLGPCPVLVVRT"
     gene            1144..2271
                     /locus_tag="VFQ79_10915"
     CDS             1144..2271
                     /locus_tag="VFQ79_10915"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEU0140217.1"
                     /translation="MHTPSMLEEKLRELALFEPTTMPVLSVYLNTQADQHGRDNFEVF
                     LRKELKNRGDSYPRRSPERQSFERDAEKVMSWLTSELRPSSNGAVIFACAGANDFFEA
                     LQFEAPIHKNRLYVYHQPHLYGLAKLHDQYPPYAAVIADTNSARIFVFGLGRVLDAEM
                     VQNDKVRSRTQIHGWWLRRYQLRVENYHLKHSKDVLQQLDKIVREEGIEHIILAGDEV
                     ILPVLRQQLSPFLAAKVVDELKLDITASERIIFQATLESMQEENARSDAEHVSAMIDE
                     YRAGGLAAVGTHDILLALANGQVDTLFVSTELEQVHPGKEDLHRALAPAVAELPVGAG
                     VRITDALVTQAYQTGAHVRFIEDPALLVNVGGVGATLRFSL"
     gene            2330..3334
                     /locus_tag="VFQ79_10920"
     CDS             2330..3334
                     /locus_tag="VFQ79_10920"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140218.1"
                     /translation="MRGKWILLAGVAILVAVIAGALSAVWRDRAERARQAQEQAEAKK
                     AGPPASEISLSGTVQAQNVVPVPASVNGTLEIYYVEEGQEVFEGQLLARIRNTRLETE
                     RDAATAELERIQTRVNNLESGIISARLEASRAMADANRARADFERVEKVYQRQRMLYS
                     EGATPRLVWEKAQKDYERTKAEYEGLQELARQAESRLSSSIGDLDQARVMLSERTAEF
                     EHAQAELQSAEVHSPVDGIVVAVRGQAGDVVDPGIPDLLRIATELNSLQVTVMPDVAS
                     LPYIHPGQPAVVQLPEAANEALPGQVLEVRDGTVVVVFQSPTPAVKPGLTAQVRLKLT
                     "
     gene            3342..>4348
                     /locus_tag="VFQ79_10925"
     CDS             3342..>4348
                     /locus_tag="VFQ79_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU0140219.1"
                     /translation="MQFLKNSLLELVTQTSTNLPPDVRTAMGIALERESAGTQSSRAL
                     GIIATNIDMAQEDEAPICQDTGMPTFIVHTPVGTNQIQIRRAIREAVAEATRNGKLRP
                     NSVDSLTGKNSGDNLGEETPVIHFEQWEQDEIEVKLVLKGGGCENKNIQYSLPCELDH
                     LGRADRNLEGVRKCILHAVWQAQGHGCAPGALGVCIGSDRGHGYHLAKMQLFRTLDDV
                     NPVPELAELEASIVQEANRLGIGAMGFGGNVSLIGCKIAAANRLPASFFVSVAYDCWA
                     FRRLGVRLDAHDGAITGWLYRDPERPVAKMAASTCFSLTGREVVLTPPLTEEAVRALK
                     VG"
BASE COUNT          951 a         1196 c         1343 g          858 t
ORIGIN      
        1 tgttgccggt gcgcaggaaa acggcagtga gttgtgttaa ccgtggcggc atcttcctca
       61 atttacgcca tacacgatgc ccgaaatggc gctgtgctgt ccgttttggt cccacggtgc
      121 gcccgccctc ggtgtgagcg gccgttgcgc gcgttgttcc tgcctgccta tgggattggc
      181 cctccatttg cacagtaagc agtttgcatg caaatactgc tatgaaattc aaaagggagg
      241 agcattgctc aaactggaga ggattctttg cccggtcgat ttttcagagt tttcggcaaa
      301 agcttacgac tacgcgcatt cgctcgcgcg gcattacggt gcgaagctgt tcgtactaca
      361 cgttactgaa cccataatat cgatctatcg cggctatatg tctgccgcaa tggtggacga
      421 aatatataca cgccaaaccg cagataccca ggaaaaggtc cgtaaactct cggagaggca
      481 ccagggaggg caggttgaat ccgacgtcgt cgtgcagttg ggaattgtat cggactcgat
      541 cctgacactc gcggaaaccc aggagatcga cctgatagtg atgggcacac acggccgccg
      601 cggcattgat cgtctggcca tgggatcgaa caccgaacga gtgttgcgaa aggctctctg
      661 cccggttttg gcggttcgcg aaccggtgcg ggattttgtg agttccgcca gtgcggtaga
      721 gccggtgcaa ctccggaaga tcgtctgttg cactgacttt tccgacaact ctcccagggc
      781 actggagtat gcattttcgc ttgccgctca gtatgaggca gaagtgactc tccttcacgt
      841 gttggaggat tcgggcgctg gtccacgcct cgaggagcaa aaggagcagg cgttcaagcg
      901 gctggaagaa gtaataccca gcgaggcgca gaactgggca actgtcatcc cattggtgcg
      961 cgccggtaaa ccctacgagg aaatcatgga gcatgcggcg cagactcaag cggacctgat
     1021 cgtaatgggc gtccgtggac gcaacgcctt ggatgtcgcg ctgttcggtt ccaccacgca
     1081 cagagtcatt caactgggac cgtgccctgt acttgtggtc aggacgtagg cacaacgagg
     1141 catatgcaca caccatccat gctggaagag aagttaagag agcttgcact gtttgaaccg
     1201 acgacgatgc cggtgttgag cgtttatctg aacacgcagg ctgatcaaca cgggcgcgac
     1261 aattttgagg tttttttacg caaagaactg aagaaccgcg gcgattctta tccgcgccgt
     1321 tcccccgagc ggcagagttt tgaacgggat gccgaaaaag tcatgtcgtg gttgacttcg
     1381 gaactccggc cgtcatcgaa tggagcggtg atctttgcct gcgcgggagc caacgatttc
     1441 ttcgaagctc ttcagtttga agcaccgatc cacaagaacc ggctctacgt gtatcatcag
     1501 ccgcacctgt acggactggc caagttgcac gaccagtacc cgccctatgc ggccgtaatt
     1561 gcagacacaa actcggcgcg aatcttcgta tttggcctcg gacgcgtcct ggatgcggag
     1621 atggtgcaga acgacaaggt cagaagccgg acacagattc acggctggtg gcttcgccgc
     1681 tatcaactac gcgtcgaaaa ctatcatctg aaacacagca aagacgttct gcagcagttg
     1741 gacaaaatcg tgcgggagga gggtatcgag catattatct tggccggcga cgaggtgatt
     1801 cttcccgtct tgcgccagca actctcaccc tttctggcag cgaaagtggt ggatgagttg
     1861 aaattggaca tcacagcctc ggagcgcatc atctttcaag ccacgctcga atcgatgcag
     1921 gaggaaaatg cgcgaagcga cgcggaacat gtgagtgcaa tgatcgacga ataccgcgcc
     1981 ggtggactcg ccgcggttgg cacgcacgat atacttctgg ctctggcaaa cggtcaggtg
     2041 gatacgttgt tcgtcagcac ggagctcgaa caggtccatc ccggaaagga agatctgcac
     2101 cgcgctctgg ctccggctgt cgcggagttg cctgtcggcg ctggtgttcg gatcaccgat
     2161 gcgttggtga cgcaggccta tcaaacaggc gcccatgtaa ggtttattga agacccggca
     2221 ctgctcgtga atgttggagg agtgggcgcg acgctgagat ttagtctttg acttcccgca
     2281 ccattgaaag cagtgcagca tgcgccgccg ccctgagtat gattgaagca tgcgcggaaa
     2341 atggatcttg ctcgcgggag tcgccatcct ggtggcggtg atcgcaggcg ccctttccgc
     2401 ggtgtggcgc gaccgggccg aaagggcacg gcaagcacaa gaacaggccg aggctaaaaa
     2461 ggcagggccg ccagcgagtg aaatcagcct ttccggaact gtacaggcac aaaatgttgt
     2521 acctgtgccg gccagtgtga atggcactct ggagatttat tacgtcgagg agggccagga
     2581 ggttttcgaa gggcaactgc tggccagaat acgcaatacc cggctggaaa cggagcggga
     2641 tgcggctact gcggaactgg agcgcattca aacgcgggtc aacaatctcg aaagcggcat
     2701 catctccgct cgccttgagg cctcccgggc gatggccgac gcgaaccgcg cccgggcaga
     2761 tttcgaacgg gtggagaagg tgtaccagcg ccagcggatg ctctacagcg agggtgcaac
     2821 ccctcgcctc gtatgggaaa aggcacagaa agactatgag aggacgaagg ccgagtacga
     2881 gggccttcag gaacttgcgc gccaggcgga atcgcggctt tcgtcgtcga ttggagattt
     2941 ggaccaggcg cgggtaatgc tcagcgaacg aaccgcggag ttcgaacacg cacaggccga
     3001 attgcagtcc gccgaagtac actcgcccgt tgacggtata gtggttgcgg ttcgaggaca
     3061 ggcgggcgac gtggtggacc ccggcattcc ggacctgttg cggattgcga cggaattgaa
     3121 ctcgctccag gtgacggtaa tgcccgatgt cgcatcgctt ccctacatcc atcccgggca
     3181 gccagcagtc gtgcaattgc cggaggccgc gaacgaggct ttgcccggcc aggttctgga
     3241 ggtgcgggat ggcacggtag tggtggtgtt tcagagtccg acgccggccg tgaaaccggg
     3301 tcttacggcg caggtgcgac tcaagctaac ataaaattcc catgcaattt ttgaagaaca
     3361 gtctgctgga actggttacc cagacctcca cgaatcttcc tccggatgta cgcaccgcaa
     3421 tgggcattgc cctggagcgg gaatcggccg gaacgcagtc ctcgcgagcg ctcggcatca
     3481 tcgccaccaa catcgatatg gcgcaggagg acgaagctcc catctgtcag gacacgggca
     3541 tgcctacgtt catcgtccac acgccggtgg gaacgaacca gatccagatc cgccgcgcca
     3601 ttcgggaggc agtcgcggaa gcgacaagaa acggtaaact gcgcccgaac tcggtcgatt
     3661 cgctgaccgg caagaacagc ggcgacaatt taggagaaga aacgcccgtc attcatttcg
     3721 agcagtggga acaggatgag atcgaagtga aactggtgtt gaagggcggc ggatgcgaga
     3781 acaagaacat ccaatattcg ctgccttgcg agctcgatca cctcggccgc gcggaccgca
     3841 acctggaagg cgttcgcaag tgcatcctgc atgccgtgtg gcaggcgcag ggtcatggct
     3901 gcgctcccgg agcgcttggc gtctgtatcg gcagcgaccg cggccacggc tatcatctgg
     3961 cgaagatgca gttgttccgc acgctcgatg acgtgaaccc ggttccggag ctggcggaac
     4021 tcgaagcctc gatcgtgcag gaagcgaacc gtctgggcat cggcgccatg gggttcggag
     4081 ggaacgtatc gctcatcggc tgcaaaatcg ccgcggcgaa tcgtctgcct gccagtttct
     4141 ttgtgtccgt cgcctatgac tgctgggcgt tccggcggct cggcgtccgg ctcgatgcgc
     4201 acgatggagc gatcacggga tggctctacc gcgatcccga acgtcccgtc gcgaagatgg
     4261 ctgcctccac ctgcttctcc ctcaccggac gcgaagtagt gctcacaccg ccgctgacgg
     4321 aagaagccgt ccgcgcgctg aaggtcgg
//
LOCUS       DASTMK010000253         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_588067, whole genome shotgun sequence.
ACCESSION   DASTMK010000253 DASTMK010000000
VERSION     DASTMK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588067"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1169)
                     /locus_tag="VFQ79_10930"
     CDS             complement(150..1169)
                     /locus_tag="VFQ79_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU0140220.1"
                     /translation="MAATMGEGSTPLVASHRIADRLGVRRLAFKLESTNPSGSYKDRF
                     IAAEMTRILASGARACVATSSGNTGSSLAAYCARYDVRCTILVNEQTPEGKLAQMKAH
                     GAKVIRIREFGSNASSTAEAFALLQEFSSSGNLPLVVSAFRYCPEGMRGVASLGREIA
                     GSVPDLGHVFVPVGGGGLFSAVCLGLEGSTARVHAVQPAGCSTVVAAWEKGGGTIVPV
                     ESNTRISGLSVPFDIDASLALTHLREHNGMGFAVEDDEVYESQRMLLEMEGIFAEPAG
                     AVALAGLRKALREGRIDPEESAVCLVTGSGFKDPESIAAVARARPERFLSNKELRQYL
                     REAVE"
     gene            1214..2134
                     /locus_tag="VFQ79_10935"
     CDS             1214..2134
                     /locus_tag="VFQ79_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0140221.1"
                     /translation="MSLHGIIPAVGTPLMDEDRVDEAGLRRLVRYVLDGGVHGVFANG
                     SMGGFAFLTDDEQVRSVAITVSAVADRVPVIGGVGETSTSRAIRLARRIAAEGVDYLS
                     VLPPFYFFATQEHLIDYFGEIAAATPVPVFLYDNPVLTKNKLEVETIIELKRRIPTLA
                     GIKVSDSDCAKLQAIVHSTREHDDFPVITGSEFLIVVALQMGCAGAVGGLHNLCPHLA
                     VALWDAYQAGDLARARSLQQDMIETWQIFRYGAIWGAFDEALRYLGICERATGRPYIT
                     VLDNAGRDAVHRILDRYVKPYLSAAAGPAR"
     gene            2343..2702
                     /locus_tag="VFQ79_10940"
     CDS             2343..2702
                     /locus_tag="VFQ79_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140222.1"
                     /translation="MRPAAVFVVCAVLLTGAVKVQSEVVDERCPGTALERGPSLIGNL
                     DPIKRVVREEGYSGQLRFRVTITDTGSVRQTAIIGPVALKESEKLRTSIEALKFCPAV
                     RHSRYASVQVNFEVQVE"
BASE COUNT          515 a          808 c          842 g          563 t
ORIGIN      
        1 aggggcgagt tgccgcgcgg cctcttcggc caattccacc cgtttctggg ccagcaccag
       61 gcgggcgcca cgttcagcga ggacccgtgc gatgccaaac ccgatgccgg ttccgccgcc
      121 ggtgacgatt gctacttttt cttcaagact cactccacgg cctcccgcag atactgacgc
      181 aattccttgt tgcttagaaa ccgctcgggc cgcgcccgtg ccacggcggc aatcgactcg
      241 ggatctttaa aaccgctccc ggtcacaagg caaacagcgg attcctccgg gtcaatgcga
      301 ccttcgcgca gcgccttccg caatcccgcg agcgccaccg ccccggcggg ctccgcaaaa
      361 attccttcca tttcgagcag catccgctgg ctctcataga cctcgtcgtc ctccacggca
      421 aagcccattc cattgtgctc tcgcaaatga gtgagggcga gagaagcgtc gatatcgaag
      481 ggcacggaaa ggccgctgat acgagtattg ctttcgacgg ggacaatcgt gccgccgcct
      541 ttttcccaag cggccacaac ggtggaacag cccgccggtt gtacggcatg tacgcgcgcc
      601 gtagagccct ccaaccccag gcaaaccgcg gaaaaaagcc caccgccgcc aacaggaaca
      661 aacacgtgtc ccaggtccgg tacgctcccg gctatctccc gcccaagcga tgcgacgccg
      721 cgcattccct ccgggcagta gcgaaacgcg gagacgacca gcggcagatt tccgctggag
      781 gagaactcct ggagcaacgc gaacgcctcg gcagtagaac ttgcgttcga tccgaattcg
      841 cggatccgga tcactttcgc accgtgggcc ttcatctggg cgagcttgcc ttcgggcgtc
      901 tgttcgttca ccagaatggt gcagcgtacg tcatagcggg cgcagtaagc ggccagtgaa
      961 gaaccggtgt tgccggaaga ggtagcgacg cacgcccgcg cgcccgacgc gaggatgcgc
     1021 gtcatttcgg ccgcaatgaa acggtctttg taagatccgc tggggttcgt ggattcgagc
     1081 ttgaaagcca gccggcgcac tcccaggcgg tcggcgattc gatggctcgc aaccagaggc
     1141 gtggaacctt ctcccattgt tgcggccatg tgccagtatt gcacttctgt cgacagtatt
     1201 ctacaatccg ctcatgtctt tgcatggaat tattccggcg gtcggcactc ctctgatgga
     1261 tgaggatcgt gtagatgaag ccggattgag gcgcctggtg cgctacgtat tggatggcgg
     1321 tgtacatgga gtcttcgcga acggttccat gggcggattc gccttcctga cggatgacga
     1381 acaggtgcgc tctgtggcaa tcaccgtttc cgccgtcgcg gatagggtgc cggtcatcgg
     1441 aggagtgggc gaaacaagta cgtcgcgtgc gatccggctt gcgcggcgaa ttgcagcgga
     1501 gggtgtggat tatctttcgg ttctcccgcc gttttatttc ttcgccacgc aggaacacct
     1561 gatcgactac ttcggcgaga tcgccgccgc cacgcccgta cctgtttttc tgtacgacaa
     1621 tcccgtgttg acgaaaaaca agctggaggt ggaaacgatc atcgagctga agcgccgcat
     1681 tcccacgctg gcgggcatca aggtgagtga ttcggattgt gctaagcttc aggccattgt
     1741 ccattcgact cgggaacacg acgattttcc ggtgatcacg gggagtgaat tcctgatcgt
     1801 cgttgcgcta cagatgggtt gtgccggcgc ggtgggcggt ttacataacc tgtgcccgca
     1861 cctcgccgta gcgctttggg acgcgtatca ggccggcgat ctcgcgcgtg cccgaagcct
     1921 gcaacaggac atgatcgaaa cctggcagat cttccggtat ggggcgatct ggggcgcgtt
     1981 cgatgaagcg ttgcgttatc tcggaatttg tgaacgggcg accggccgcc cctacattac
     2041 ggtgctcgat aacgccggac gggacgccgt acaccggatc ctggaccgtt atgtgaagcc
     2101 gtatctaagt gcggcggccg gacctgcgcg ttgagaccgg cgagccggcg cacggccgcc
     2161 acttcccgca cggcgtgaac gtaagcggtg agggggcggt tgattccttc ctccgtcccg
     2221 gtgtcatagg gaggtttgcc gtagagctct cgcagccgcg agatccagga catcggattt
     2281 ggcgtcatga gcggcagcgc cacggattga ctaaccgtgc gtggagggtg atagtggtct
     2341 gtatgcggcc ggctgcggtg ttcgttgtct gcgctgttct gttgacgggc gcggtcaagg
     2401 tgcaatcgga agtcgtggac gagcgctgtc cgggcactgc attggagcga ggcccgtcct
     2461 tgatcggcaa ccttgatccg atcaaacggg tggttcgtga ggaaggctat tcaggccagc
     2521 tccggtttcg ggtcacgatc acagataccg ggagcgttcg tcaaactgcc atcatcgggc
     2581 ccgtagcctt gaaagaatcg gagaaactgc gtacttccat cgaagctttg aaattctgtc
     2641 ctgctgtcag gcacagccgc tacgcatcgg tccaggtcaa tttcgaagtt caggttgaat
     2701 agtgcctcgg cgcttgctga cgcgcttc
//
LOCUS       DASTMK010000254         9837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_459168, whole genome shotgun sequence.
ACCESSION   DASTMK010000254 DASTMK010000000
VERSION     DASTMK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459168"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFQ79_10945"
     CDS             complement(<1..711)
                     /locus_tag="VFQ79_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140223.1"
                     /translation="MFPGINGFHWTVGHVIFLGAFFTVLMVIGTTLVRAWLRTRKDLA
                     ANRVEALRWAADFGDLSPSERACRHAITETAVGRVCNNRFDCRVCETHPKLAALEVGR
                     EAGPVENEIFGLEFPADRLYHRGHTWAKTEPGGEVTVGLDALAERLAGKPDGIELPRP
                     GERLVVNGTAWKMRKLGTEIRVLSPVEGEVLETGGPAAGWYLKIKPDSSAGDAHLLRG
                     AEVQPWVMRELERLQLAFA"
     gene            complement(724..1956)
                     /gene="nrfD"
                     /locus_tag="VFQ79_10950"
     CDS             complement(724..1956)
                     /gene="nrfD"
                     /locus_tag="VFQ79_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU0140224.1"
                     /translation="MDRGLIIRGVRRCSFGRFALWMVPWALLLIVGCYAAFLCLYEGL
                     HHTNMDNRFAFGLWIFLDLTVIALGAGAFFTGFLLYIMKRKELQAVINSAVVIGFICY
                     SGAVAVLAVDVGQPLRAWFTFWHPNTHSMLTEVTFCISVYLTVLAIEYLPVILKNRKL
                     RQVPSFLVFEFQLHKLMYVLAGVGTFLSFFHQGSLGGLYGVLHARPFAFREGFAIWPS
                     TFFLFILSAAAVGPSFILLTTWLVSKVTRKELVKPAVFRSLARVSGVLLIVYVLFKSI
                     DTLIWINGTSPGLGFAAYDYYAWKPFGTWILFTEIIVLGLIPALLLLNEKRLPNRGWL
                     ILAAILASSGVALNRFVLTIQTLALPTLPFDQFLSYWPSWQETATFLAVVAYGVLVYS
                     FSFRYLTLFPQEKELSRI"
     gene            complement(1949..2725)
                     /locus_tag="VFQ79_10955"
     CDS             complement(1949..2725)
                     /locus_tag="VFQ79_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0140225.1"
                     /translation="MTKETAKIKPARYGMVIDLDRCNGCGACMIACAVENNVPACPEG
                     VTERTGLTWMRVQKLGNGQSFPDSRTAFIPVMCQQCGDKTPCVSVCPQQAVEVDEASG
                     IVDQMPQRCLGCRYCMVACPYHTRYFNWWDPAWPNGMERTLNPDVAPRMRGVVDKCNF
                     CHGRYQEARAKAAVDGTPERAVEYTIACAEACPTGAITFGDLDNPESEAAQLAKSENA
                     FRLLEKLGTGPKVHYISKRAWVRAAAGPSLAADTREVRNG"
     gene            complement(2722..4752)
                     /locus_tag="VFQ79_10960"
     CDS             complement(2722..4752)
                     /locus_tag="VFQ79_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0140226.1"
                     /translation="MSLGRRDLLKFLGGAAAGAIFTPVPWKLIDDVSIWTQNWSWLPR
                     VPRGEIQSKYTTCTLCSSGCPVRVRCAGAQPVSMWGAAGHRLCPIGLSAHHLPYHSRR
                     LARPMERTQAGQWQPVALEAAVERISNAVSGCMGLRGSLAIVDGRAGTTASLLYKRFV
                     AQTSAAVYIADETGARNGIDLDRTRTLLSLGVPLQDGWGEPGRVMRLQADGKLTVIHA
                     EAKASATASLADTWLPLAPGSETAFALALAHVILKDGLYDRSRMQKAEDFRRFETLAA
                     AFPPERVAAVTGLAPEVIGGVARQLAQQGPSVVLAESRPGVEGADELATAVAALNLLI
                     GSEGREGGILARPEIPVHDDLKDAARVPATMLVAVPDRSVKVLWLDDADIPWAALERK
                     LAPEHLVISVSSWPTGAAAHAGLFVPGPLFLESVRDAAAPADSAAAWFRITNPLLPAP
                     PESREGVDLLSQVAEKIGLTPTDSLADHLHRRAEAIRTAGHGQVVSYADGTATDTAAF
                     SSADDFWNALANGGAWTQAASAKAPSRLRLPGGSASFESMMRAASPPPAANPGTAAEL
                     PLVVGAYAGPPAASPLLSKLYEESGLKELPGAALIHPQTAERAGLKPGARAMMQAGNV
                     KGPVLVEISASVMPGVLHVSAAPDFHPARPKHGIAETAAANGRLVRARIWNA"
     gene            complement(4762..5349)
                     /gene="qrcA"
                     /locus_tag="VFQ79_10965"
     CDS             complement(4762..5349)
                     /gene="qrcA"
                     /locus_tag="VFQ79_10965"
                     /EC_number="1.97.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF041781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone reductase multiheme cytochrome c
                     subunit QrcA"
                     /protein_id="HEU0140227.1"
                     /translation="MKHSGKIAFSAGLLLALHLGWRQFPDVLYSKTDQPLQFSHKVHV
                     EDSGMSCEDCHSVRDDGRFTGIPPIAKCAECHAEPLGTTPHEKVLVEKYVATLTEIPW
                     HVYSRQPDNVYFPHAAHVKTAKLACESCHGEHGKTENLRPFEQNRISGYSRDIWGPSI
                     SRISLKGERLGMKMDDCIDCHTEKKLRHSCLDCHK"
     gene            complement(5346..6287)
                     /locus_tag="VFQ79_10970"
     CDS             complement(5346..6287)
                     /locus_tag="VFQ79_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140228.1"
                     /translation="MTCPFLREAQVKFCQVSPFRKMLVRSAAHGTDEKCTSPGYLGCS
                     VYVETGHQGPTQNRCPFFGESLTQYCSIAPVPKYIPYSESMLCRCGTESYRYCEMYLT
                     MARPAGGDGPALPLDARSQIGPPAEEWIDDMRVPNWLLYTTNHLWVDRTESGNCHVGI
                     DAVLAKALGKVDSLNFAVSGEHPSVILEAGGAEFEVTFPRILQMRRVNAHLRLDPNPA
                     TADPYGSGWLFEGSLSGKESAGLIPGNAAAGWMRSEVERLSHHVHEHILPADPESAGL
                     AADGGSFAPGLLRIVGRERASVLFNEFFGPYASWKKR"
     gene            complement(6314..6598)
                     /locus_tag="VFQ79_10975"
     CDS             complement(6314..6598)
                     /locus_tag="VFQ79_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140229.1"
                     /translation="MDDPKTLWLNLTNIALGLVVLVCIAVVAYGVLQEFALRRKKRAA
                     AFVEIDRDMKNLAAAAAHDDHAFHLPELGLTMADGGERTDSPKRAPRKKK"
     gene            complement(6897..8291)
                     /locus_tag="VFQ79_10980"
     CDS             complement(6897..8291)
                     /locus_tag="VFQ79_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated pathogenicity-related family
                     protein"
                     /protein_id="HEU0140230.1"
                     /translation="MKRLTLRTCSVFLSLLIAVAAPRQTAHPTALDKYVHAPDSSYTY
                     RPVATLPGKGYTVHVLDMTSQSWRSPAEVDRTLWKHWLNVIVPDRVESETGLLFIGSG
                     SNRSKPPEKADVMLANIAVATHSVVAELRMVPNQPLTFSDDGKPRVEDEMIAYTWDKF
                     LRTGDESWPARLPMTKAAVRAMDTVTAYVAGLPAKVKVDRFVVSGASKRGWTTWTTAA
                     VDDRVVAIMPIVIDMLNIEPSFIHHYRVYGFWAPAVHDYVDMGIMDWQGTPQYQALMK
                     LEEPYSYRDRLTMPKYLINSAGDQFFLPDSSQFYFDDLRGEKYLRYVPNTDHSLRNSD
                     APESLLAYYSAFLRNEPRPEFTWKFEKNSSIRVETKSTKPAAVKLWQASNPEKRDFRL
                     EMIGRAYQATDLVPAKEGVYVAKITPPAKGWTASFIELTYSSGGKVPFKFTTPIRVLP
                     DRLPFEAPRPARKK"
     gene            complement(8325..9479)
                     /locus_tag="VFQ79_10985"
     CDS             complement(8325..9479)
                     /locus_tag="VFQ79_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEU0140231.1"
                     /translation="MLLFRWQFVADFIVLAVALYILLRWAKRAQATRLALGILGLHAA
                     AVAARYLDLFLTSWVLDAAAIVAVLALLVIFQAEVRRAFLRLDTLLRLRTQQKRTVAS
                     AHRTIAETAFRLAAEKTGALLVLVRNDSIAELMDGTIELRANISSEILEAIFQKNSPL
                     HDGAAVIDGDQIVYANAVLPLTQRADLPVMYGTRHRAAMGLTERSDALVIAVSEERGD
                     VSLVRRGEVLPMSDARDLRYRLQAADGKPPARVTSRLRRALTSNLRLKFAAGGIAAVL
                     WILSVYATGTTVRTIRVPVEFRNVPSGMNVIAQSAESIDVQLRGRAWLMDTEGVRSLV
                     AHFDLSKAQQGPLTLLIGANQLDLPPGIELETVTPRVLSVMIERRQEPKP"
     gene            complement(9525..>9837)
                     /locus_tag="VFQ79_10990"
     CDS             complement(9525..>9837)
                     /locus_tag="VFQ79_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140232.1"
                     /translation="SGMWVTTGRNADPQFVLAPGEFRNAAFQVYRRNSGNPTGLSYTF
                     DVSLEQLEILPSQQIRSVRQFSLNFPDLTVSGMRGGAQPAENLNDAGKKLIGIIRGKK
                     K"
BASE COUNT         1925 a         3027 c         3008 g         1877 t
ORIGIN      
        1 tgcgaatgcg agttgcagcc gctcgagctc cctcatcacc cacggctgca cttccgcgcc
       61 gcgcagcagg tgggcatcac cggcggagga atccggtttg attttcagat accagccggc
      121 ggccggaccg cccgtttcca acacctcccc ttcgaccggc gacagcacgc ggatttcggt
      181 tcccagcttc cgcattttcc atgcggttcc gttcaccacc aggcgctcgc cgggccgcgg
      241 cagctcgata ccgtccggtt ttccggccag tcgctccgcg agggcgtcca aacccacggt
      301 tacctcgccg cctggctccg tcttcgccca ggtgtggccg cgatggtaga ggcggtccgc
      361 gggaaactcc agtccgaaaa tctcgttctc aaccggtccg gcctcgcggc ccacttcgag
      421 cgcagccagt ttcgggtgtg tttcacaaac acggcagtcg aaccggttgt tgcagacgcg
      481 gcccacagcg gtttccgtga tggcgtggcg gcacgcccgc tccgagggag aaagatcgcc
      541 gaaatcggcc gcccatcgga gcgcttctac acgatttgca gccagatcct tgcgcgttcg
      601 gagccaggca cgcacaagag tggtcccgat gaccatgagc accgtaaaaa acgcgcccag
      661 gaatatgaca tgccctactg tccagtgaaa accatttatg cccggaaaca tagatcatct
      721 ccctcaaatt cttgaaagct ctttttcttg cggaaacaac gtcagatacc ggaaagagaa
      781 cgaatagacc agcactccat acgccaccac agccaggaac gtcgcggttt cctgccagct
      841 tggccagtaa gaaagaaact ggtcgaaggg aagcgtcgga agcgccaggg tctgaatcgt
      901 caggacgaac cggttcagcg ccacaccgga cgaagccagt attgcggcga ggatcaacca
      961 tcccctgttc ggcaggcgtt tctcgttcaa caacagcagc gcgggaatca gccccagcac
     1021 gatgatttcg gtaaacagaa tccaggtgcc gaaaggcttc catgcgtagt agtcgtaggc
     1081 ggcgaagccg aggccggggg atgtgccgtt aatccagatc agtgtgtcga tggatttgaa
     1141 cagaacatag acgatcagca acacgccgga aacgcgcgcc agcgaacgga acacggcggg
     1201 cttcaccagt tccttgcggg tcactttcga aacgagccac gtcgtgagca gaatgaagct
     1261 cggtcccacg gccgcggccg aaaggataaa cagaaagaag gtggagggcc agatggcgaa
     1321 gccttcccgg aacgcgaacg gccgcgcgtg taacacgccg tacaaccctc cgagtgagcc
     1381 ctggtggaag aacgaaagaa acgttcctac gcccgccagc acgtacatca gcttgtgcag
     1441 ttggaattcg aatacaagga acgatggaac ctggcgaagt ttccggtttt tcaggatcac
     1501 cggcagatac tcgatggcga gcacggtcag gtagacgctg atgcaaaagg tgacctcggt
     1561 cagcatcgag tgcgtgttcg gatgccagaa ggtaaaccag gctcgcagcg gctgccccac
     1621 atcgacggcc agaacggcca cggcgccgct atagcagatg aatccgatga ctacggcgct
     1681 gttgatcacc gcctgaagct ccttgcgctt catgatgtag agcaggaacc ctgtgaagaa
     1741 ggcgccggcg ccaagagcga tcaccgtgag gtccagaaag atccagagcc cgaaggcgaa
     1801 ccggttgtcc atgttggtgt gatgcaagcc ctcataaagg caaagaaagg cggcgtagca
     1861 tcccacgatg agcagcagcg cccaggggac catccataac gcgaaccgtc cgaaactgca
     1921 ccggcgtact ccccggataa tgagtcccct atccattgcg gacctccctg gtgtcggccg
     1981 ccagcgacgg gccggcggct gcgcgcaccc aagcgcgttt tgagatgtag tgaactttgg
     2041 gcccggttcc gagcttctcg agaaggcgga aggcgttttc cgatttcgcg agttgagccg
     2101 cctcgctctc gggattgtcg aggtcgccga acgtgatggc tccggtggga caggcttcgg
     2161 cgcacgcgat ggtgtattcc accgcgcgtt ccggcgtacc gtccaccgct gctttcgctc
     2221 gtgcctcctg ataacgcccg tggcagaaat tgcacttgtc gacgacgccg cgcatgcgcg
     2281 gcgcaacgtc cggattcaag gtgcgttcca ttccattcgg ccaggcggga tcccaccagt
     2341 tgaagtacct ggtgtggtaa gggcaggcga ccatgcagta gcggcagccc aggcaacgct
     2401 gcggcatctg gtctacgatg ccgctggcct catccacttc cacggcctgc tgcgggcaca
     2461 cggagacgca gggtgtcttg tcgccgcatt gctggcacat gaccggaatg aacgcggtcc
     2521 ggctgtccgg gaacgattgg ccgttaccga gcttctgcac gcgcatccag gtgagacctg
     2581 tgcgctcggt cacgccctcc gggcacgccg gaacgttatt ctccacggca caggcgatca
     2641 tgcaagcgcc gcagccgttg caacggtcca ggtcaatgac cattccgtac cgcgccggtt
     2701 ttatcttggc tgtttctttc gtcatgcgtt ccagatcctc gctcgaacaa ggcgcccgtt
     2761 ggcggcggcg gtctcagcga tgccgtgctt cggccgcgcc gggtggaagt cgggagcggc
     2821 cgaaacgtgc aggacccccg gcatcaccga cgcgctgatt tcgaccagca ccgggccctt
     2881 cacgttaccg gcttgcatca tggcgcgggc gccgggcttc aggcccgcgc gctcggccgt
     2941 ttgcgggtgg atcaaggcgg cgcccggcag ttccttcagg ccggattcct cgtacagctt
     3001 gctcaatagc ggcgatgccg cgggagggcc cgcataggcg ccaacgacca gcggtaactc
     3061 cgcggccgtc cccggattgg ccgccggggg aggggaggcg gcgcgcatca ttgattcaaa
     3121 actcgcggac ccgccgggaa gccgaagccg cgacggagcc ttcgcggaag ccgcctgggt
     3181 ccatgcgccg ccgtttgcca gcgcgttcca gaagtcgtcc gccgaggaaa aggcggcggt
     3241 atcggttgcc gtgccatccg cgtacgaaac cacctgcccg tgtccggctg tgcgtatcgc
     3301 ttcggcgcgc cggtgcagat gatccgcaag actgtccgtg ggtgtgagtc caattttttc
     3361 ggccacctgg ctcaacagat cgacgccttc cctcgattcg ggcggagcgg gcagcagcgg
     3421 attggtgatc cggaaccatg cggcggcgga gtctgccggc gcggcggcgt cccgcacgga
     3481 ctcgagaaag agcggaccgg gcacgaacag ccccgcgtgt gcggcggcgc ccgtgggcca
     3541 ggacgacacg ctgatcacca aatgttccgg tgccagcttg cgctccaggg ccgcccacgg
     3601 aatgtccgcg tcgtcgagcc agagaacctt tacggaacga tcgggcaccg ccaccagcat
     3661 ggttgccgga acgcgggcag cgtctttcaa gtcgtcgtgt acggggatct ccggcctcgc
     3721 gaggatgcct ccttccctcc cttcgctacc gatcaacagg ttcagcgcgg caacggcggt
     3781 tgcaagctcg tccgcacctt cgacaccggg ccggctttcc gccagcacaa cagagggacc
     3841 ttgctgtgcg agctgccggg caacaccgcc gatcacctcc ggcgccaggc ccgtgaccgc
     3901 ggctacgcgt tccggtggaa acgcggcggc cagtgtttca aaacgtcgga aatcctctgc
     3961 cttctgcatc cggctgcggt catacaaacc gtcttttaga atcacgtgcg ccagagccag
     4021 cgcgaaggct gtctccgagc ccggcgccag cggcagccag gtatcggcca gagaggccgt
     4081 ggcggacgct ttcgcttccg catggatgac ggtgagcttt ccgtccgcct ggagccgcat
     4141 cacacggccg ggctcacccc agccgtcctg aagcggaact cccaggctga gcagcgtcct
     4201 ggtcctgtct agatcgatcc cattgcgggc gccggtttca tcggcgatat aaacggccgc
     4261 gcttgtttgt gctacgaaac gcttgtacag aagcgaagcg gtagttcccg cccggccgtc
     4321 gacgattgcc agtgaaccgc gcagtcccat gcatccggat accgcgttgg agatccgctc
     4381 cactgccgct tcgagcgcga cgggctgcca ctggccggct tgtgtgcgct ccattggccg
     4441 tgccagccgc cgcgaatgat agggcaggtg gtgggcgctc aacccgatgg gacataaccg
     4501 gtggccggcg gctccccaca tgctgacggg ttgcgcgccg gcgcaccgca cccgcaccgg
     4561 acagccggag gagcacagag tacaagtagt gtatttcgat tggatctcgc ccctcggcac
     4621 gcggggcagc caggaccagt tctgcgtcca gatggacacg tcatcgatca gcttccaggg
     4681 caccggcgta aaaatggcgc cggcggctgc gcccccgagg aatttcaaaa gatcacggcg
     4741 tccgagactc atttcctatc ctcacttatg gcagtcgaga caactgtgcc ggagtttctt
     4801 ttccgtatga cagtcgatac agtcgtccat cttcattccc agccgttccc ccttcagcga
     4861 tatgcgggaa atggaaggac cccagatatc gcggctgtat ccgctgatcc ggttttgctc
     4921 gaagggccgc aggttttctg tttttccatg ttccccgtgg cagctttcac aagccagctt
     4981 ggccgtcttc acgtgcgcgg cgtggggaaa atagacgttg tccggctggc gggagtaaac
     5041 gtgccagggg atctccgtca acgtggctac atatttttcc accagcacct tctcatgggg
     5101 cgtagtgccc aggggttcgg cgtggcactc ggcgcacttg gcaatgggag gaatgcccgt
     5161 aaaccgcccg tcgtcacgga cggagtggca gtcctcgcag gacatcccgc tgtcctcgac
     5221 gtggactttg tggctgaact ggagcggttg atcggtcttg ctgtacagaa cgtcggggaa
     5281 ttgccgccac cccagatgaa gggccaatag caggcccgcg gaaaacgcaa ttttgccaga
     5341 atgtttcatc gtttcttcca gctcgcatat ggtccaaaaa attcgttgaa gagcacggac
     5401 gcccgctccc tgccaacaat tctgagcagg cccggggcga atgagcctcc atccgcggcc
     5461 agacccgcgc tctcgggatc ggccggcagg atgtgctcat ggacgtgatg cgacagacgc
     5521 tccacctcgg atcgcatcca gcccgctgcc gcgttgccgg ggatgaggcc cgccgattcc
     5581 ttgccggaca ggctgccctc gaagagccag cccgaaccgt aggggtcggc ggttgccgga
     5641 ttgggatcca accgcaggtg agcgttcaca cggcgcatct gaagtattcg cggaaaggtc
     5701 acctcgaact cggcgccgcc tgcttcgagg atgacagagg ggtgctcgcc ggacaccgcg
     5761 aagttcaaag agtcgacctt gcctaacgct ttggccaaca ccgcatcgat gccgacgtgg
     5821 cagttgccgc tttccgtcct gtccacccat agatgatttg tggtgtagag cagccagttg
     5881 ggaacgcgca tgtcgtcgat ccactcttcc gcgggtggcc cgatctgaga gcgggcgtcg
     5941 aggggcagcg cgggtccgtc tccgcccgcc ggccgggcca tggtaaggta catctcgcaa
     6001 taacgataag actcggttcc acaccggcac agcatcgatt cgctgtacgg aatgtatttc
     6061 ggaaccggtg ctatcgagca atattgggtt aacgattcac cgaagaaagg acaccggttc
     6121 tgagttggtc cctggtgccc ggtttcgacg tacaccgaac agccgagata gcccggcgac
     6181 gtgcattttt cgtcggttcc gtgagccgcg ctgcgaacca gcatcttccg gaatggagat
     6241 acctggcaaa acttcacttg cgcttcacgc aaaaaaggac aggtcatacc ttccccccgc
     6301 tcgcgtccgc ccgctacttc ttcttgcgcg gcgcccgctt cggcgaatcc gttctttcac
     6361 cgccgtcagc catggtcagg cccaattcag gaagatggaa agcatggtcg tcgtgagctg
     6421 ccgcggcggc caggttcttc atgtcgcggt cgatttcgac gaatgcagcg gcgcgtttct
     6481 tgcggcgcag agcgaactcc tgaagaactc cgtacgccac caccgctatg cagaccagga
     6541 ctaccaggcc cagtgcgata ttcgtcaggt tcaaccacag tgtcttcgga tcgtccatta
     6601 cggcgaacct cctttcgagt ctccacacct cggagatcag taagagtgca atcgccgggc
     6661 cgaaccgcta tgtcattgat aatggctgga aaatgccgga cgggcaggga aatacagctg
     6721 ctccatgtag gattttaaga ctttaaaatc ccaaaatccc acacagcccc ctctgctgct
     6781 tgctattaaa catgacgtcc taaatgtttg cgcgacgatt tgattggcca cggatggaca
     6841 cagatgcaca cggatctgtg tgaatccgtg ttcatccgtg gctaaatttg ttctgtttat
     6901 ttcttgcggg ccgggcgtgg ggcctcgaag ggaagccggt cgggaaggac tcgaatgggt
     6961 gtggtgaact tgaaaggaac cttgccgccg gaactatagg tgagttcgat gaacgaggcc
     7021 gtccagcctt tcgccggcgg ggtgattttt gccacataca cgccttcctt cgccggaacc
     7081 agatccgtag cctggtaggc acggcctatc atttccagcc ggaaatctct cttctcagga
     7141 ttcgacgcct gccagagttt caccgcggcg ggcttcgttg acttcgtctc aacgcggatg
     7201 gagctgtttt tctcgaactt ccaggtgaac tccgggcgag gttcgtttcg tagaaacgcg
     7261 ctgtaatatg ccagcaggct ctccggcgcg tcggagttgc gcagcgaatg gtccgtgttc
     7321 ggcacatagc gcagatattt ttcgccgcgc agatcgtcga agtagaattg cgaggaatcg
     7381 ggcaggaaga actggtctcc cgcggaatta atcaggtact tcggcatcgt cagccggtcg
     7441 cggtaggagt aaggctcctc cagtttcatc aacgcctggt actgaggagt tccctgccag
     7501 tccatgatgc ccatgtccac ataatcgtga acggccggcg cccagaagcc gtagacgcgg
     7561 tagtgatgaa taaacgacgg ctcgatgttc agcatgtcga ttacgatcgg cataatcgcc
     7621 accacgcgat cgtcgacggc ggcggttgtc cacgtggtcc agccgcgctt ggaagcaccg
     7681 gacaccacga agcgatccac cttcaccttc gcgggaaggc ccgctacata ggcggtcacc
     7741 gtatccatcg cgcgcaccgc cgccttggtc atcggcaaac gggcgggcca ggattcatcg
     7801 ccggtgcgga gaaacttgtc ccaggtatag gcaatcatct catcttcgac acgcggcttg
     7861 ccgtcatcgg aaaatgtcag cggctggttc ggaaccatac gaagctctgc aacgaccgag
     7921 tgtgttgcca cggcgatgtt cgcgagcatc acatcggcct tctccggggg cttcgagcga
     7981 ttgcttccgc tgccgatgaa cagcaggccg gtttcggact ccacgcgatc cggaacgatc
     8041 acgttcaacc agtgcttcca taaagtgcgg tctacttccg ccggactacg ccaggattga
     8101 gaggtcatgt cgaggacgtg aaccgtgtag ccctttccgg ggagggttgc aaccgggcgg
     8161 taggtgtagc tcgaatccgg cgcgtgcaca tacttatcga gcgcggtcgg gtgggccgtc
     8221 tggcggggcg ccgcaacggc aatgagcaag gagaggaaaa cggagcaagt cctcaacgta
     8281 aggcgtttca tgccggcttc caaatgacca gtgtacgtct gcgactatgg cttcggttcc
     8341 tgccggcgct cgatcatgac ggacagcact cgtggggtga cggtttccag ttcgatcccc
     8401 gggggcaggt cgagctggtt cgcgccgatc aacagagtga gagggccctg ttgcgccttg
     8461 ctgagatcga agtgcgccac cagcgatctc accccctcgg tgtccatcag ccacgctctg
     8521 ccgcgcagtt ggacatcgat gctttccgcc gactgggcga tgacgttcat cccgcttggc
     8581 acgttccgga actccacggg cacgcgtatg gtgcgtacgg tggtgccggt ggcgtacacg
     8641 gagaggatcc agagtaccgc cgcgatgccg cccgcggcaa acttgagacg caggttggat
     8701 gtgagcgcgc ggcgcaaacg gctggtgacc cgggccggag gctttccgtc ggctgcctgg
     8761 agacgatacc gaagatccct ggcatcgctc atgggaagga cttccccgcg ccgtaccagt
     8821 gagacatcgc cccgttcttc cgatacggca atcaccaggg cgtccgagcg ttccgtcagg
     8881 cccatggcgg cgcggtggcg cgttccgtac atgacgggca agtccgcccg ttgcgtgagc
     8941 gggagcaccg cattggcata gacgatttga tcgccgtcga tcaccgcagc gccgtcatgc
     9001 agcggtgagt tcttctggaa aatcgcttcc aggatttcgg acgagatatt ggcccggagc
     9061 tcgatggtgc cgtccatcag ctcggcgatc gaatcgttcc ggaccaggac gaggagagcg
     9121 cccgtctttt cggcggcaag ccggaatgcg gtttcggcaa tggttcgatg ggcggaggca
     9181 accgtccttt tctgctgtgt tctcaaccgg agcagcgtat ccagacgcag aaaggcccgg
     9241 cgcacttcag cctgaaaaat caccaacagc gcgagcacgg cgacaatggc tgccgcgtcc
     9301 agcacccaac tggtgagaaa caggtcgagg tagcgagccg ccaccgccgc cgcgtgcaga
     9361 cccaaaatgc ccagggccag gcgtgttgcc tgcgcccgtt tggcccatcg caacagaatg
     9421 tatagcgcaa ccgcgagaac gatgaaatcg gcgacgaact gccagcggaa cagcaacatg
     9481 gaatcttcca ctccatgtta gcgggggcct tttcggcctt ccagttattt ctttttcccg
     9541 cgaatgatac cgatcagttt ctttccggca tcgttgaggt tttcggccgg ctgcgcgccg
     9601 ccccgcatgc ccgagacggt gagatcggga aagttcaaac tgaactggcg gacggagcgg
     9661 atctgctggc tgggcagaat ctcgagttgc tccaggctga cgtcgaacgt atacgacagt
     9721 ccggttggat tgccgctgtt ccggcggtac acctgaaacg ccgcattccg gaattctccg
     9781 ggagcgagga cgaattgcgg gtcggcgttt ctgccggtgg tcacccacat gccactg
//
LOCUS       DASTMK010000255         9042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_524804, whole genome shotgun sequence.
ACCESSION   DASTMK010000255 DASTMK010000000
VERSION     DASTMK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524804"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFQ79_10995"
     CDS             complement(<1..458)
                     /locus_tag="VFQ79_10995"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0140233.1"
                     /translation="METVLDSWKSIRDDTAQAVEEFPSGAFDFRPTDGLMTFGEIARH
                     ILEAGHALTGMMLAGVEDFTVPGFREMLNEHLPALPEGPDGAQLALALRTELDARISD
                     LSKQPEAFFSHMMTRFDGMRVTRFEMLQFVKEHELTHRAQLFLYMRLKGLV"
     gene            complement(504..938)
                     /locus_tag="VFQ79_11000"
     CDS             complement(504..938)
                     /locus_tag="VFQ79_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEU0140234.1"
                     /translation="MNKAYRQGQILSLIRSRTIYTQEELARELKSVGVPATQVTLSRD
                     VRELGLVKTAQGYRQIRPETGGPPLSTIAAEFLWEIRAAQNLIVLKTSPAHASSLAAA
                     LDRQQWPEIVGTVAGDDTVLVVTPDNDAADALRKRLLPFISP"
     gene            complement(935..2317)
                     /gene="argH"
                     /locus_tag="VFQ79_11005"
     CDS             complement(935..2317)
                     /gene="argH"
                     /locus_tag="VFQ79_11005"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU0140235.1"
                     /translation="MKLWGGRFEKGPSEVFERFSGSLHFDRRLIEADVRGSQAFARAL
                     ERVGILTPAEREQIVSAFDKIAEEARQESFFEGAGDEDVHTLVIRKLKERVGPLADKI
                     HTGRSRNEQVSLDTRLWIRSECRRIQSLLAGLMGALVAHGRRYPDTIVPGYTHLKRAQ
                     AVFWPHYLLAYFEMFARDFARFQRAHDSAGLMPLGSGALAGSGFPFDRREIASDLGMD
                     AVTLNSMDVSGDRDFALDFLFAASTVMLHLSRLAEDWILYSSDEFGWLELSDEVTSGS
                     SLMPQKKNPDSLELIRGKSGRVIGNLVSLLVTMKGLPMTYNRDMQEDKEQLFEAADQV
                     SASLEMARAVVDSARLNPTAPAKAVEESWVVATDLAEALARAGTPFHRAHALAGGLVL
                     ESVRHGKKPSEWTEQELAAFAPEFTPDMIRLLNPAEGMKTREISGGTGPAAVQKALDE
                     AEARLKTMSE"
     gene            complement(2314..3525)
                     /locus_tag="VFQ79_11010"
     CDS             complement(2314..3525)
                     /locus_tag="VFQ79_11010"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU0140236.1"
                     /translation="MNKPKKVALAYSGGLDTSIIMPWLKENYGCEIVAVCGDIGQGSE
                     ELAGLEEKARKTGASEVYVEDLREEFVTEYLWRLVRSGGVYEHKYLLGTSIARPLLAK
                     RQVEVALRTGCDGLAHGCTGKGNDQVRFELTYKALAPHLQVIAPWREWDIVSREDAIE
                     YAHKHNVPISQTTTKIYSRDRNIWHISHEGGALEDPANAAPDDIWMLTKSPNQAPDTP
                     AEVTIGFNQGRPVSLNGQHQMSGVGLLERLNQIGGEHGIGRIDLVENRFVGMKSRGCY
                     ETPGGTLIMAAHRELEALTLDRTTLHYKQKLALDYAEMVYNGFWFTPLRESLDAFFEA
                     ASRNTTGEVRLRLYKGNIEPVSRKSPNSLYSLNIASFTMGAEYDQKDALGFINLIGLP
                     IKTRASISRKS"
     gene            complement(3545..4489)
                     /gene="argF"
                     /locus_tag="VFQ79_11015"
     CDS             complement(3545..4489)
                     /gene="argF"
                     /locus_tag="VFQ79_11015"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0140237.1"
                     /translation="MATPISASPVWKSSAQDFARDLDLSTEEVTNLLDLAREMKRHPA
                     RFRTALSGRYLSLLFEKPSLRTRLTFELAMKQLGGDSIVSNGPIAAREPLKDVARNLD
                     RWTNGIVARTFLQSTIDELAHWSSVPVINALSDLFHPCQALADVLTLQEHLGELRGKK
                     IAFLGDGNNVAHSLMITATRLGMDFALAHPEGYAPSPEVVSDAAAGAAISGASIAITT
                     EPREALAGAHAVYTDVWASMGQEDEAGERQKRFEDYQVSAERFAQARPDAVFMHCLPA
                     KRGQETTDEVMESPRSVVFDQAENRLHAQKALLLMILG"
     gene            complement(4565..5563)
                     /locus_tag="VFQ79_11020"
     CDS             complement(4565..5563)
                     /locus_tag="VFQ79_11020"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEU0140238.1"
                     /translation="MRRRELLALAPGSLLAAASHAQPAAPQLPRAAFRLATGFPVPDP
                     FCMRTKEGWYLTGTQHARGIDDRRFSLFFSPDLKTWRDLGPILVRPEYEGSHKANYWA
                     PEFMEHGGTYYLYYTSDSFGDPERRFVRVATSSRLTGPYRDSGDRLVTKPSIDGHPYF
                     VSSGEGWLFYCGNEGNPHVGQLLVDRFLSPSQLENKPRKVFPNETVAWEEGAFIIRRL
                     KVYYLFSSMGNWRNGTYHVRVARSRSIEGPWERLMENGAPYRVLQTVEGQSGAGHNSL
                     FRGPDGDWWICYHAWDPGKTGRYPWAAPLSWDSRGYPAVQQLPEASSGQKPGLQGA"
     gene            5747..6850
                     /locus_tag="VFQ79_11025"
     CDS             5747..6850
                     /locus_tag="VFQ79_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0140239.1"
                     /translation="MIHTRRELFHASLLTAAASVRPAAAQGKAQLGMPGLFPGRVVTT
                     HHPAALVSGAYQPAAVEQMMHKGMTELTGAPGWPDAWRAFFEPGDVVAIKVCPVGGRR
                     ICSDSLVLNRIIDGLKQAGVKQGDIFVFNRYRREMFSAGMPQWVPEGVRLAWASENYN
                     KIQQDIEGYDPEMFVELALAVPGQDIREERVRRSYVARLLSKQVNKVINLPVLKHHNA
                     AGVTITLKNLSHGFVNNVERSHPTPTLNATGTFIPAVLNLPVFREKIVLHIVDGVRAQ
                     YEGGPGGRPQFMWEHNTMYFGTDPVALDKTGWKVIDAKRVEQGLPPVAEAKLSKHNIM
                     VSTQVEHIELSGALGLGRFDDAAIDVKRFELNA"
     gene            6887..8113
                     /locus_tag="VFQ79_11030"
     CDS             6887..8113
                     /locus_tag="VFQ79_11030"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEU0140240.1"
                     /translation="MIRRSSILRIFLPVALAMIAPGQQPPELFHKRPGSHPRWITFEN
                     PKGVKGGGALENKGAKGHPADPWPAGQTKTLMDLPGAGVVRHIWLTIPNRDPKTLRSL
                     RVEMYWDGAARPAVSAPLGDFFGAILGRTIPLENEFFTNPEGRSFNCYIPMPFRKRAL
                     ITVTNESGRDLRWIFYQIDVEMLDTPDPNALYFHAFWHRVRWTELGKDFEILPRVAGE
                     GRFLGTHFGLIGHPANVGWWGEGEVKIYLDGDTGAPTLVGTGTEDYIGTGWGQGLFQN
                     RFQGSVVNDADNRQWAFYRYHVPDPVYFRKDLRVTIQQMGGSEKENVLKLLKAGVEVQ
                     PVTIDWDGKFTKLLEEPGPVDLEKHPSSAKAWVNMYRRDDASAIAFFYLDKPENELPP
                     LPPVADRVVGILESAK"
     gene            8233..>9042
                     /locus_tag="VFQ79_11035"
     CDS             8233..>9042
                     /locus_tag="VFQ79_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0140241.1"
                     /translation="MTVLTVALLSAACFAPAAGAPRTDFQFPQTYPPTNEVRMDNMVA
                     IPMRDGVKLYADIYRPAADGKYPVIVCRTPYSTERYPSAYDAAVYFSRRGYVFVYQDV
                     RGRHESEGKWEPFRNDLEDGYDTIEWAAKQPWSNGKVGMQGGSYLGHVQWRAAMMSPP
                     HLVAIFPSVASTSLYHDWITLNGGWRLSFNFGWGPVRQESRIMQNTGPHTMAGGPESI
                     SFEKTLWHLPLFNMQELLGRNAQFYRDWIEHPDYDDYWKKLNAEEVFDQIAI"
BASE COUNT         1778 a         2759 c         2690 g         1815 t
ORIGIN      
        1 acaagacctt tgaggcgcat gtaaaggaag agttgcgcgc ggtgagtgag ttcatgctcc
       61 ttcacgaact gcagcatttc gaaacgcgtt accctcatgc cgtcgaatcg ggtcatcatg
      121 tgcgagaaaa acgcctcggg ctgtttggaa agatctgaaa tccgcgcatc aagctcggtc
      181 cgtagagcca acgcaagctg cgccccgtcc ggaccctccg gcaatgcggg cagatgctca
      241 ttcagcatct cgcggaatcc gggaacggtg aaatcctcca ctccggcgag catcattccg
      301 gtcagagcgt gccctgcctc gagaatatgc ctggcgattt cgccgaacgt catcaggccg
      361 tccgtgggcc ggaagtcgaa ggcgccggat gggaattcct ctactgcttg cgcagtgtca
      421 tcccggatgg atttccagga atccagtact gtctccactc gcaccatatc cgccattgta
      481 aaggacggcc accaggcttt cagctacggt gaaataaacg gaagaagccg tttgcgcaga
      541 gcgtccgccg cgtcattgtc cggcgtgacc accaatacgg tatcgtcacc ggcaacggtg
      601 ccaacaatct ccggccactg ttgccggtcc aacgccgccg cgagggaact tgcgtgtgcc
      661 ggcgaggtct tcaagacaat caggttctgc gccgcccgga tttcccatag aaattcggcg
      721 gcgatcgtgg aaagcggcgg cccgcctgtc tcggggcgaa tctgccggta accctgcgct
      781 gtcttgacaa gcccaagctc acgcacatcg cgcgatagcg ttacctgggt ggcgggtacg
      841 ccgacagact tcaattcgcg ggccagttcc tcctgtgtgt atatcgtccg cgagcggatc
      901 agcgataaga tttgcccctg ccggtaggcc ttgttcattc gctcatcgtt ttcagacggg
      961 cttcggcttc atccagtgcc ttctgcaccg ccgccgggcc ggttccacct gagatttccc
     1021 gtgtcttcat gccttcggcc ggattgagga ggcggatcat gtccggcgtg aactccggcg
     1081 cgaaagccgc cagttcctgc tccgtccact ccgagggttt ctttccatgg cgcacgcttt
     1141 cgagaaccag gccgccggcc agggcatgcg cgcggtgaaa gggcgttccc gcccgcgcca
     1201 gggcttccgc cagatcggtg gccaccaccc agctctcttc gacggccttt gccggcgccg
     1261 tgggattcag ccgcgcggaa tcgaccaccg cgcgtgccat ttcaaggctt gcagacacct
     1321 ggtccgcggc ctcgaagagc tgctccttat cttcctgcat gtcgcggttg taggtcatcg
     1381 gcaggccctt catcgtcacc agcagcgaaa ccagattgcc gatcaccctg cccgattttc
     1441 cgcggatcag ctccagcgaa tcgggatttt tcttttgcgg catcaggctg gatccgctgg
     1501 tgacctcgtc gctcaactcg agccacccga attcgtcgct cgaatagaga atccagtctt
     1561 cggccagacg gctcaggtgt agcatgaccg tggaagcggc gaacaggaag tcgagcgcga
     1621 aatcgcggtc gcccgagaca tccatgctgt tcaaggtcac cgcatccatc ccgaggtcgc
     1681 tcgcaatttc ccggcggtcg aaagggaacc cgctgccggc cagcgcaccc gagccgagag
     1741 gcatcagccc ggcgctgtcg tgggcgcgtt ggaaccgcgc aaagtcccgt gcgaacatct
     1801 cgaaataagc gagcagatag tgcggccaga atacggcctg cgcgcgcttc aggtgcgtgt
     1861 agcccggcac gatcgtgtcc ggataacggc gcccgtgggc gactagcgca cccatgagcc
     1921 cggccagcag gctctgtatc cggcggcatt ccgaacggat ccagagacgt gtatcgagcg
     1981 agacctgctc gttgcggctg cgccccgtgt gaatcttgtc cgccagcggt cccacccgct
     2041 ccttcagttt ccggatcacc agcgtgtgta cgtcttcgtc cccggcgcct tcgaagaagc
     2101 tttcctggcg ggcttcttcg gcgatcttgt cgaacgccga cacaatttgc tcgcgctccg
     2161 ccggtgtcag aatcccgacg cgctccaggg ctcgcgcaaa agcctgcgag ccccgcacgt
     2221 cggcttcaat cagccggcgg tcgaaatgca gagagccgga gaagcgctcg aagacctcgg
     2281 aggggccttt ctcaaaacgt ccaccccaga gcttcatgat ttccggctga tggaggcccg
     2341 cgtcttgatc ggcaacccga tgagattgat gaatcccagg gcgtctttct gatcgtattc
     2401 ggcgcccatg gtgaagctgg cgatattcag cgaataaagc gaattgggag acttccggct
     2461 gaccggctcg atgtttccct tgtacaggcg aagccggact tcaccggttg tgttgcggct
     2521 cgccgcttcg aagaaggcgt cgagcgactc ccgcagcggc gtgaaccaga agccgttgta
     2581 caccatctca gcgtagtcga gcgccagctt ctgcttataa tgcagcgtcg tcctgtcgag
     2641 cgtgagcgcc tcgagttccc ggtgcgcggc catgatcagc gtaccgcccg gcgtttcgta
     2701 gcagccgcgc gatttcattc cgacgaagcg gttctccacc aggtcgattc gtccaatgcc
     2761 gtgttcgccg ccgatctggt tcaacctctc cagcaggccg acaccagaca tctggtgttg
     2821 cccgttgagc gataccgggc ggccctggtt gaagccgatg gtcacctcgg cgggcgtgtc
     2881 cggcgcctgg ttcggactct tcgtgagcat ccagatgtcg tccggcgccg cgtttgcagg
     2941 gtcctccaat gcgccgccct cgtgcgagat gtgccagata ttccggtcgc ggctgtagat
     3001 ttttgtggtg gtctgcgaaa tgggaacatt atgcttgtgc gcgtattcga tcgcatcttc
     3061 ccgcgacacg atgtcccatt cgcgccatgg cgcaatcacc tgaagatgag gtgccagcgc
     3121 cttataggtc agctcgaaac ggacctgatc gtttcccttg ccggtgcagc cgtgagcgag
     3181 tccatcgcaa cccgtccgga gcgcaacttc gacctgtctt ttcgccagta gcggacgagc
     3241 gatggaggtt cccagcaggt acttgtgctc gtaaacgccc ccggagcgga ccagacgcca
     3301 caagtactcc gtgacgaact cttcgcgcag gtcttccacg tacacctcgg aagcgccggt
     3361 ttttcttgct ttttcctcaa ggcccgccag ttcttcggag ccttgtccga tgtcgccgca
     3421 aacggcgacg atctcgcagc cgtagttttc tttcaaccac ggcatgatga tggaggtatc
     3481 cagaccgccc gagtaggcga gcgccacttt cttaggttta ttcatagaaa attcggggtg
     3541 gaagctagcc gagtatcatc agcagcaaag ccttctgcgc gtgcaggcgg ttctcagcct
     3601 gatcgaacac gaccgagcgc ggcgattcca tcacctcatc ggttgtttcc tgaccgcgtt
     3661 tcgccggcag acagtgcata aaaacagcat cgggccgcgc ttgcgcaaat cgttcggcgc
     3721 taacctggta atcctcgaag cgtttctgcc gttctccggc ctcatcctcc tggcccatgc
     3781 tggcccacac gtctgtgtaa accgcgtggg ctcccgccag ggcctctcgc ggttcggtgg
     3841 taatcgcaat cgaggcgccc gagatcgcgg caccggcggc cgcgtcgctc acaacctccg
     3901 ggctgggcgc gtatccttca gggtgcgcca gggcaaaatc cattccaagc cttgtggccg
     3961 taatcatcag cgagtgggct acgttgttcc cgtcgccgag gaaggcgatt ttctttccgc
     4021 gtaattcgcc caaatgttcc tgtagcgtca gcacgtccgc cagcgcctgg cagggatgga
     4081 aaagatcgct cagggcgttg atcaccggta ccgaagacca gtgcgccaac tcgtcgatcg
     4141 tactttgcag aaacgtgcgg gcgacaatgc cgttcgtcca ccggtccagg ttgcgggcaa
     4201 catccttcag cggctcccgc gcggcaatcg gcccgttgga cacgatagag tcaccgccaa
     4261 gctgcttcat ggcaagttca aaggtgagcc gtgttcgcag cgatggcttt tcaaacagca
     4321 ggctgaggta acggccgctc aacgccgtcc ggaatcgagc cgggtgccgt ttcatctcgc
     4381 gagccagatc caaaaggttc gttacttcct cggtggagag gtcgagatcc cgcgcgaaat
     4441 cctgcgcaga ggacttccag accggactgg ctgatattgg cgtggccacc ctgtcgctcc
     4501 ctttcatgct gttaactgaa ttaatattca gataagtgaa taattacaca tcgaggtggg
     4561 cgagtcaagc tccctggagc ccgggctttt gtccgctact tgcttccggg agttgttgca
     4621 cggcagggta accgcgggag tcccaggaga gtggcgccgc ccaagggtag cggcctgttt
     4681 tgccgggatc ccaagcgtga tagcagatcc accagtcgcc atcgggcccg cggaacagcg
     4741 agttatgccc cgcgcccgac tgtccctcga cggtctggag aaccctgtac ggcgcgccgt
     4801 tctccatcag ccgctcccac ggcccctcaa tgctccggga gcgcgcgacg cgcacgtgat
     4861 aagtgccgtt gcgccagttc cccatgctgg aaaataagta gtaaaccttt aggcgccgga
     4921 tgatgaacgc accttcttcc cacgcgacag tctcgttcgg aaagactttc cgaggcttgt
     4981 tctcgagctg cgacggggaa aggaaccggt ccacgagaag ctgcccgacg tgtgggttgc
     5041 cctcgttgcc gcagtagaac agccagccct cgccggaact gacgaaatac ggatgcccat
     5101 caatggaagg cttggtcacg agcctgtcgc ccgaatcgcg gtacggaccc gtgagccggc
     5161 ttgaggtggc aacccgcaca aagcggcgct ctgggtcgcc aaacgaatcg gacgtgtagt
     5221 agaggtagta agttcctccg tgttccatga actccggggc ccaatagtta gccttgtggc
     5281 ttccctcgta ttcgggacgc acaaggatcg gaccgaggtc ccgccaggtt ttcagatccg
     5341 gtgagaaaaa cagactgaag cgccggtcat cgattccgcg ggcatgctgg gttcccgtca
     5401 ggtaccagcc ttccttggtc cgcatgcaaa acgggtcggg aaccggaaaa cctgtcgcaa
     5461 gccggaaggc cgcccggggc aactgcggcg cggcaggctg tgcgtgcgag gccgccgcaa
     5521 gcagggaccc aggggccagc gctaggagtt ccctgcggcg cattacccga tcttgtgagt
     5581 tcacactcgt gtattttatc gcgctggccc gcgaaggcac tcggcggcga gtcgccgatg
     5641 acagtgttcc gatttgtccg gccgtgtaaa agttaataca cttgtccaca agccaaccgg
     5701 gccgccattg cgcctccaaa aggctatggc cagcaggaga atatttatga ttcacactcg
     5761 ccgcgaactg ttccacgcgt cgctgcttac ggcagcggct tccgttcggc cggccgccgc
     5821 acaaggcaaa gctcaactgg gaatgcccgg cttgtttccg ggccgtgtag tcacaacgca
     5881 ccatcccgcc gcccttgtct cgggagccta tcagcccgcg gcggtcgagc agatgatgca
     5941 caaagggatg accgaactga ccggcgcacc gggatggccg gatgcctggc gcgctttttt
     6001 cgaacccggc gacgttgtag cgatcaaagt ttgccctgtc ggaggccgcc gcatctgctc
     6061 cgacagtctg gttttgaatc gcatcatcga cggtttgaag caggcaggcg tcaagcaggg
     6121 cgacattttc gtcttcaatc gttatcgtcg tgaaatgttt tcggcaggta tgccgcagtg
     6181 ggtgccggaa ggggtgcggc tcgcctgggc gtcggaaaac tataacaaga ttcagcagga
     6241 tattgaaggc tacgaccccg agatgttcgt cgaactggct ctggccgtgc cggggcagga
     6301 catccgcgaa gagcgggttc gccgttccta cgtcgcccgc ctgctcagca agcaggtaaa
     6361 caaagtgatc aatcttcccg tgctgaagca ccacaacgcc gccggagtca ccatcacgct
     6421 gaaaaacctg tcccacggat tcgtcaataa cgtggagcgc agccatccca cgccgacgct
     6481 caacgccacg gggactttta ttccggcggt gctgaatctg ccggttttcc gggaaaagat
     6541 cgttctgcat attgtggacg gcgtgcgcgc ccagtacgaa ggcggacccg gaggcaggcc
     6601 acagttcatg tgggaacata acaccatgta cttcggtacc gatccggttg cgctcgataa
     6661 gaccggctgg aaagtgatcg atgccaagcg cgtcgagcag ggcctgcctc ccgtggcgga
     6721 agccaaactc agcaagcaca acattatggt atcgacgcag gtggaacaca tcgagttgtc
     6781 cggagccttg gggcttggcc ggttcgacga tgccgctatc gatgtgaagc ggttcgagtt
     6841 gaacgcttag acccattcca aacagacgcc gtgaccgagg agatccttga tacgccgaag
     6901 ctctattctt agaatcttcc tgcctgtcgc cctggcgatg atcgcgccgg ggcagcagcc
     6961 gccggagctt tttcacaaac gtccaggtag ccacccccgc tggatcacct ttgagaatcc
     7021 caaaggagtg aaggggggag gcgcactgga gaacaagggc gcgaaaggac acccggccga
     7081 cccatggccg gcagggcaga ccaaaacgct gatggattta ccgggcgccg gcgtggtgcg
     7141 gcacatctgg ctcaccatcc cgaaccgcga tccgaaaacg ctgcgctccc tgcgtgtcga
     7201 aatgtattgg gatggcgcgg cgcgtcccgc tgtctccgct cccttgggcg atttcttcgg
     7261 cgccattctc ggacgcacca taccgctcga aaacgaattt ttcaccaatc ccgaggggcg
     7321 ctccttcaac tgctacatcc cgatgccgtt ccgcaaacgc gccctcatca cggtgacgaa
     7381 tgaaagcggg cgcgatctgc gatggatctt ctatcaaatc gatgtcgaga tgttggatac
     7441 accggatccg aatgcgctct atttccacgc gttctggcac cgggtgcgat ggacggaatt
     7501 gggcaaagat ttcgaaattc ttccgcgcgt cgcgggcgaa ggccggttcc tgggaacgca
     7561 tttcggcctt atcggccatc ccgcgaacgt cggatggtgg ggtgagggcg aggtcaaaat
     7621 ctatctcgac ggggacacgg gcgcgcccac cctggtaggc accgggacgg aggattatat
     7681 cggcaccgga tggggccagg gtctgttcca gaaccggttt cagggaagcg tcgtgaacga
     7741 tgcggacaac cggcagtggg cattctatcg ctatcatgtg cccgatcccg tttatttccg
     7801 caaagatctt cgcgtcacaa tccagcagat gggcggctcg gaaaaagaga acgtcctcaa
     7861 gctgctgaaa gcgggtgttg aggttcagcc ggtaaccatc gattgggacg gcaaattcac
     7921 aaagttgttg gaggagcccg gtccggtgga tctggagaag catccgtcgt cagcaaaggc
     7981 gtgggtgaac atgtaccgcc gggacgacgc ttcggcgatc gcgtttttct atctcgacaa
     8041 accggaaaac gaattaccgc cgcttccgcc ggtagccgat cgtgtagtag gaatactgga
     8101 atctgcaaag tgaccgaaag ccagggacgc gggatctgcc ggcttgcaga accgctcgcg
     8161 gcccgggcgc gttgtcagac tcattcaatt gttgataggc tcttatgcca aagcatgatt
     8221 gacagacagc cagtgaccgt acttacagtc gcgctgctaa gcgccgcgtg tttcgcaccg
     8281 gcagccggcg cacctcgaac cgatttccag tttccgcaaa cgtatccgcc gacgaatgag
     8341 gtccgcatgg acaacatggt tgcgatcccc atgcgggatg gcgtcaagct ctatgccgac
     8401 atctaccggc ccgcggcgga tggcaagtat ccggtgatcg tttgccgcac gccttacagc
     8461 acggagcggt atcccagcgc ctacgatgcg gccgtttact tctcgcggcg cggctacgtc
     8521 ttcgtgtatc aggacgtgcg cggccggcat gaatcggaag gtaagtggga gccgttccgg
     8581 aacgatctcg aagacggcta cgacaccatt gagtgggcgg caaagcagcc ctggtccaat
     8641 ggcaaagtag ggatgcaagg cggctcctat ctgggacacg tacaatggcg cgcggccatg
     8701 atgtcccctc cccacctggt agccattttc ccgagtgtcg cctctaccag cctctatcac
     8761 gattggatca cgctgaacgg aggctggcgg ctctcattca atttcgggtg gggccccgtc
     8821 cggcaggaat cccggattat gcagaatacc ggcccgcaca cgatggccgg cgggccggaa
     8881 agtatcagct tcgagaaaac gctctggcac cttccgctgt tcaacatgca ggagctgctc
     8941 gggcgcaatg cgcagtttta tcgcgattgg atcgagcatc ccgactacga cgactattgg
     9001 aagaagctga acgccgagga ggtgttcgat caaatcgcca tt
//
LOCUS       DASTMK010000256        17601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_268235, whole genome shotgun sequence.
ACCESSION   DASTMK010000256 DASTMK010000000
VERSION     DASTMK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17601
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268235"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..3602)
                     /locus_tag="VFQ79_11040"
     CDS             complement(132..3602)
                     /locus_tag="VFQ79_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140242.1"
                     /translation="MSRKPGARGALFGLLVAWCVLPLGAQEYRGRVQGFVTDPSNAAV
                     TGADVTLTNQGTGIAVTRQTTENGMYLFDLVQPGAYSIAVRAAGFNAFEQKGIIVQTR
                     GDVTVNASLQVGSMTETIEVTADATTVEFNNSSMSQTVIGKMLDQMPVIARNPFNLAV
                     LNPAVVNRYNNAANSLPFYQISHAGMDIGGATGGRIQLLLDGTDTRVEQRGSYAPSMD
                     AVQEVVVQQNPVDAEYGNSAGGAISIAMKSGTNELHGTGYYYGRNPYFNAVPSAITRT
                     PNKARNHIGGGVVGGPVWIPKIFDGRNKAFFFVSYEKWDSSTVSGLSQMTLPTDLERQ
                     GDFSQSLNQFGNLRTIYDPMTTVLDPATNTARRTPFPGNRIPGSLIDPSAKALMSYVY
                     QPNNPGEPFTHLNNWKASPRVGNPYNNLSVRGDYQFNTAWRAFGRYSRQNQTQIPEAL
                     ESSPAYSSWSAARMYALNLAGNVDGMLSPTWLVNFRFGYIRSADELDIPIGKVDTDFW
                     KSIWPNEWWRQSLSQSPVIYFPAFSVDGAAFGNASGWNLLPRQFNYSGTAMKQTTSHY
                     LKFGAKLSHYTSNSGLPNWGTFAFTGATTASTYINPPTDVSGAGWASFLLGHVNSGYT
                     NYAAPIWTISNNIGLFFQDDWKVSRNLTLNLGIRWEYDQAPREHDGNLGRYLDLSDPI
                     PEFQTNPVTFPDLAKYGGFPARLTGAYQFLDSDNPRVFNAPKDVFLPRFGAAYRIDDK
                     TSVRFGYSRYSIPFQTSLGPNWNLPNAGFSQRTDALAPLQGVPRTVLSDPFPVANPVL
                     APMAKSYGRYHQLGNAITYYNQYPKQPINNRINLSVQRELPRRIRLDVTFFTNLGQNL
                     PNSTVWGNAVQGEQNNMMDPALSYQFKSELDTRVDNPFYNYLTPDKFPGSLRYQQTVS
                     IGSLLKPYPQYGNLTEMYTPGINDLYRSLQIKVEKSYSSGLSFSFGYNYGRAKRDRYF
                     NSDAQYARDYTMMDTLDFRHNLTAAPYWELPFGKGRRWATSLHPVLEAVVGGWTTSHL
                     LSFRTGNLLSWGQLKVNGDPRVSNPSRDRWFNTQAFAPAEPYTPRTNPLYYEGLTGPV
                     FWNLDSTLAKNFQLTEGLRFELRMDAFNLPNHFLGGNPDMGVRSQTFGRALTQANFGR
                     EMQYTIRLHF"
     gene            3722..4024
                     /locus_tag="VFQ79_11045"
     CDS             3722..4024
                     /locus_tag="VFQ79_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEU0140243.1"
                     /translation="MRGTMVTAFVLINTAQGRTPDIAQALIDLPGITEAYSVAGPYDL
                     VAVARVRHHEELANLVAGRVQKIPGIETTNTLIAFQAYSQRDLEAMWDIGNTEPAG"
     gene            complement(4103..5245)
                     /locus_tag="VFQ79_11050"
     CDS             complement(4103..5245)
                     /locus_tag="VFQ79_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140244.1"
                     /translation="MNTGMQPAGSMRLFRAFAGIGAMAIVAGTFLAPARMSSNLLLVS
                     YFLLGIGLAAAFFVALEYVTGAGWSVALRRVPEAASSALPAGAAGLILFFILGSWLYP
                     WTHEQPAGFKALWLDLPFFVARAVIYLAVWMGFAWAVIRNSRRQDADGDLVFSRRNTR
                     LSVIFMFAFAVTFSLASFDWIMALEPHWASTIFGVYNFAGLFSSGLAVIILLVIRLRN
                     HGPLRDFVNQEHLHDLGKLLFAFSTFWMYIWFSQYMLVWYANITEEAVYYVRRQQGLW
                     TPLFLLNMLLNWAIPFLVLLPKSTKRNPGILAKVAVVVLAGRWLDLYLMIVPAVSEKP
                     AFGIWEAALGLGALGVFMLAFTRAFSSSAPVPVRDPNLIESLHYHN"
     gene            complement(5242..5766)
                     /locus_tag="VFQ79_11055"
     CDS             complement(5242..5766)
                     /locus_tag="VFQ79_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0140245.1"
                     /translation="MNRLNAFLLAALVVLTGATYMARSHPEKPNYEFIPDMMRTAAYD
                     SFSANENFADGKTLQAPVPGTIPFREERPATKPAPERGQAVFATFCQPCHGATGKGDG
                     AVSMRGFPPPPSLLAPNAMDLTDEKMFEIVSAGFKNMPGYAAQIEPEDRRQVIAYIRS
                     MQRAAAGAQQEGRP"
     gene            complement(5763..6296)
                     /locus_tag="VFQ79_11060"
     CDS             complement(5763..6296)
                     /locus_tag="VFQ79_11060"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEU0140246.1"
                     /translation="MNRRVLIGIFDDEEDVLGATRAVREQGLKIVDVYAPYAVHGLDK
                     AMGLQPSRLPWVCFLLGLFGAAAKVWFEYWTTAYNWPVNVGGKPWDSLPAFVPITFEV
                     MVLFAGLSTVFAFFLVSRLRPGRKVKPVHPRVTDDRFAVVIEETDALFDPVRVQEALE
                     KFNVVHVEERELEGSPV"
     gene            complement(6286..7665)
                     /gene="nrfD"
                     /locus_tag="VFQ79_11065"
     CDS             complement(6286..7665)
                     /gene="nrfD"
                     /locus_tag="VFQ79_11065"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU0140247.1"
                     /translation="MANVYSVLRTPLVEGNKRLSNVTEDVCSAMDRRPGALWWAGFLL
                     AVSMLLVGVAAVSYQVATGIGTWGLNNTVGWAFDITNFVFWVGIGHAGTLISAVLFLL
                     RQRWRTSVNRSAEAMTLFAVMCAGIFPLIHMGRPWFAYWVFPYPNFRGPLWINFRSPL
                     AWDAFAISTYFIISATFWYLGLIPDLATLRDRLKSGLRKRIYSFFSLGWDGSYRTWQR
                     YETVYMLLAGLATPLVLSVHTIVSWDFATSVIPGWHATIFPPYFVAGAIFSGMAMVLT
                     LMIVARKTMHLEDYITARHVETMCKLVIATSGMVGLAYATEFFIAIYSGNEYERFVFV
                     NRAMGPFAWAYWIMVTCNVAVPLLLWFRKVRTHMGAVFVVSLLVNVGMWFERFVIIVT
                     SLHRDYLPSNWTGYRPTVIEVATLLGSFGLFFTCFLLFCRLLPVIAIGEVKGVLSYGR
                     SNGGGRHES"
     gene            complement(7658..10612)
                     /locus_tag="VFQ79_11070"
     CDS             complement(7658..10612)
                     /locus_tag="VFQ79_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0140248.1"
                     /translation="MRNSKMPEELQYWRSLEEREAGVPASEAGGAEFASTPLSILEPD
                     AGRRAFLKAAGFTFAGAFLAGCSRAPEQKAMPHLVQPEGAVAGRPIYYASTCGACTAG
                     CGILVKTRDGRPIKLEGNPRHPLSRGGLCAVGQASILGLYDSLRLKHPILRGKQASWE
                     EADREVAARLDAIRQRGGAVRFLSQSVTSPTTLSAIGRFLAGFKDSRHVAYDALSSSA
                     ILDAHQVTHGTRILPHYRFENADVVVSFDADFLGTWISPVEFTRAHSERRNPEHRGSR
                     PVSYHIQIESRLSVTGSKAGRRIRIAPDETVPRLRALLTAVVTGDRADAVASRLLAAR
                     GRSLVVCGTQDVEAQTLVNRINQELGNYGSTLDIARPSRQRQGNDGELHSLLDEIAAN
                     KVSALFLHGVNPVYDLPDGAALAAQLEKLPLVVSFTERVDETASKAHVVCPVPHFLET
                     WSDAEPVAGIVSIGQPAIQPLSEARTVAESLSAWTGSPRSARDLVREQWQGRLNWEDG
                     LREGFARVDRKESPSRWIAGAANPAPRQQEATPDGLHLVLYPKPSMLDGKHAYNPWLQ
                     ELPDPITKATWDNYASIAPALAQTLDVRDGDVLQLERNGRTLELPAVIQPGQHERVIA
                     VALGYGRTETARFSSVGPRWFSAGSSTGENGLVGKNAAHLASFAGGVLRFESGGLQVK
                     KTGKRQELAITQSHHTVAPPPNLAPASGGVRPIVQETTLEVLAHNEPHAHAAHGDLWP
                     PDHAYHGRHWGMAVDLNACTGCSACVIACQVENNIPVVGKDEVRRKREMHWIRIDRYY
                     SGDGEDVTVAHQPMMCQHCEHAGCETVCPVLATVHSSEGLNQQIYNRCVGTRYCANNC
                     AYKTRRFNWFDYPREDKLANMVLNPDVTVRSRGVMEKCTFCVQRIQDAKFEAKRRGEP
                     LRDGDFETACQQSCPARAIAFGDKNDPDSAVSRLVRGNREYTVLEEINLKPSVHYLKL
                     VRASGVEEERHG"
     gene            complement(10612..11265)
                     /locus_tag="VFQ79_11075"
     CDS             complement(10612..11265)
                     /locus_tag="VFQ79_11075"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU0140249.1"
                     /translation="MSARIVTLVLLIGLAYTLMLLGWRTSSFHLPENQQDYEPVQPIA
                     YSHRLHAGELQIACQYCHFGAEKSRHAGIPAASVCMNCHRFVTAPLGALREEDELAKS
                     EKRPPRAIVSAELQKLYDALALNAKMERDPAKQTQPIAWTKVHHLPDFVYFDHRPHVN
                     AGVTCQECHGPVETMERVRQVPDLSMGWCVNCHRNANRTGVAGKPVQASIDCSACHF"
     gene            complement(11262..12578)
                     /locus_tag="VFQ79_11080"
     CDS             complement(11262..12578)
                     /locus_tag="VFQ79_11080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0140250.1"
                     /translation="MNKLRGTPAQGLFGATLGFFIGFAAVALFGPTATRFQEVMQLSP
                     LAVGFLVAMPALSGSLLRIPFAAWVDTNGGRKPFLVLLSLSIAGMSGLAAVVYFLYPQ
                     RLDASLYPLILLLALLSGCGIATFSPGIGQVSYWYTQQTQGRALAVFAGVGNLAPGLF
                     TFLLPLAIAAAGLAGSYLIWLGLLVSGTVLYALLGRNAWYFQLRERGLSPDEARKQAM
                     ERGQALFPAGSLKDSLRISAGVWRTWALVLVYFTTFGGFIALTAWLPTYWRGNFDVSP
                     ITAGLLTAAFSILTSLIRIAGGILSDQLREGGENTAILALFIMLVGSLILTGSNQFEL
                     ALPGELLLAIGMGISNAAVFKLVPQAVPQAVGGAAGWVGGLGAFGGFVIPPIMAFAVR
                     DLGRPGYHIGFIVFVFLTLVSLTMVWILKYARVESPAKLSTERSAA"
     gene            complement(12575..13279)
                     /gene="narI"
                     /locus_tag="VFQ79_11085"
     CDS             complement(12575..13279)
                     /gene="narI"
                     /locus_tag="VFQ79_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEU0140251.1"
                     /translation="MTSAWTYLDQLLFVALPYVALFTFFLVTIQRYRMRRYSYSSLSS
                     QFLENQQHFWGLVPFHYGILVILAGHIIGFLIPREVLAWNSKPLRLYVLEVTALIFGL
                     LTLAGLAACIMRRFSVSKVKQVTSRADWLLFVLLLVQVGSGVAVALFHPWGSSWFASS
                     AVPYLWSIAGLNPNVAYIAPMPFMVKLHIVNAWLLIGFFPFTRLVHVLVVPNPYLWRK
                     PQVVRWYGKPEAGRAQ"
     gene            complement(13272..13781)
                     /locus_tag="VFQ79_11090"
     CDS             complement(13272..13781)
                     /locus_tag="VFQ79_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140252.1"
                     /translation="MSLRALFCYPEEDYKALVAACARDATGTPAAAPLVVFAAALEPL
                     STTDVQEKFVQTFEMNPDTTLDIGWHLYGEQYERGEFLVTARRALRRYEVEESAELPD
                     HLSHLIPLIDRMDTEERAAFTTEYLAPALTKILSGLKGNIFENLVAAVARLYAPEPSL
                     VGAEGNGHD"
     gene            complement(13778..15268)
                     /gene="narH"
                     /locus_tag="VFQ79_11095"
     CDS             complement(13778..15268)
                     /gene="narH"
                     /locus_tag="VFQ79_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEU0140253.1"
                     /translation="MNVRSQISMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMWWNNV
                     ETKPGTGFPTAWEDQEKYRGGWEVAEGKLQLRSTSKQKTVFNIFHNPHMPTMDDYYEP
                     WTYDYKELFDAPEGPDQPTARPISMVTGELINIESGPNWDDDLGGSPVYAENDRNLEG
                     LTPEQRQQLFAVERLVFFYFPRICNHCLNPSCVASCPSGALYKRGEDGIVLIDQNRCR
                     AWRACVSACPYKKVFFNWFTGKSEKCILCFPRIETGQAPACFHSCVGRIRYLGVLLYD
                     ASRIEEIAKLPDEQLVEAQRSIILDPFDPEVIAGAQANGVHSSVIEAAQKSPVYQFVK
                     VWGIALPPHIEYRTLPMLFYVPPMAPVMATKEGATVNHVSENLFHDIDEARIPMKFLA
                     SLFGAGHEGKVRYALRKQKAVRWYRRAVTVGDVDMATAERMLREAGSTVEEAEAIYKL
                     TSLCTFEERFVIPPSHREEAIEMMRDPLEHKQNAGFGFLTGPRRGA"
     gene            complement(15286..>17601)
                     /locus_tag="VFQ79_11100"
     CDS             complement(15286..>17601)
                     /locus_tag="VFQ79_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0140254.1"
                     /translation="SGEVVTVATVYDLMMAQFGVPRGLEGAYPESYDDEQAPYTPAWS
                     EKYTGMDRQMVIRFAREWGNTARLTNGKCTIIIGAGINHWYHNNLMYRAGMYALMFCG
                     CIGVNGGGLAHYVGQEKLAPGESWSSIAFGKDWYPPSRLQNAPSWHYIHTNQWRYEKE
                     FTDYHNVPQHARPGSFAHGHTADRQVEAVRYGWLPFYPQFDRSSLAIVKDAEAAGAQS
                     ADDVVRYTVDQLKSRNLKFAVEDPDAPENWPRVWFIWRGNALMSSAKGHEFFLKHYLG
                     THTNAISEEVAAGSVNQIAWHENAPQGKMDLVVDLNFRMDTSALYSDIVLPAATWYEK
                     ADLNSTDMHSFIHPLSAAVPPCWESKTDWQIFRTIAKKFSELAARHFPEPVQDLVATP
                     LAHDSAAEIAQTKIGDWMSGEVEAIPGKTMPALKVVSRDYRHLYDQYISFGPKVAENG
                     LGAHGTHYEVRDLYEEARERGPVVSWNGGSYPSLFEDEQVCDTILRFATVTNGELAYR
                     SYKNMERKVGLPLAHLAEKSRSVRAGYKDLQSRPHRFVNSPMWSGLVEGGRPYSAFTY
                     NVECLVPWRTLTGRQHFYLDHPGYIDFGEHLPTFKPKPLPVQYGDLQFSDRVGKTMML
                     NYLTPHGKWHIHSTYGDNQRMTTLSRGVEPFWMNDKDAEELGIVDNDWVEVHNDNGVV
                     VTRAAVSARIPRSICIQYHSPERTYSVPKSPLRNYRRAGGHNSLTRVRLKPNLMVGGY
                     GQFTFHFNYWGPTGCNRDTHVLVRKLPDLKW"
BASE COUNT         3525 a         5367 c         5393 g         3316 t
ORIGIN      
        1 tcgcgatatt ggggacggca atcctgagtt taagtgtgat gcgcttccat aagcgcctgg
       61 attgaagact ccactccctc acggtcgtgg ctcagtacgg agccgcgacc gcgagggagt
      121 ggttatcgtg tttagaagtg cagccggatc gtgtactgca tctcgcggcc gaagttggct
      181 tgcgtcaggg cccggccaaa agtctggctc ctcacgccca tgtcggggtt gccacccagg
      241 aagtggttgg gcagattgaa agcgtccatg cgcaattcga accggagccc ctcggtgagc
      301 tggaagtttt tggccagcgt gctgtccagg ttccagaaca ccggtccggt caggccttcg
      361 tagtagagcg ggttggtccg cggcgtgtag ggctcagccg gcgcgaacgc ctgcgtgttg
      421 aaccaccggt cccggctcgg attcgacacg cgcgggtcgc cgtttacctt caactggccc
      481 cacgaaagca ggttaccggt gcggaaagag agcaaatggc tggtcgtcca tccgcccacc
      541 acggcttcga gtaccgggtg caggctggtg gcccaccggc ggcctttacc gaacgggagc
      601 tcccagtagg gcgcggcggt gaggttgtgg cggaagtcca gcgtgtccat catcgtgtaa
      661 tcccgggcat actgcgcgtc gctgttgaaa taccggtccc gcttggcgcg gccgtagttg
      721 tacccgaagc tgaagctcaa cccgcttgag taggacttct ccaccttgat ctgcaaggag
      781 cggtagagat cgttgatgcc cggcgtgtac atctcggtca gattcccgta ttgcggatag
      841 ggcttcagca ggcttccgat gctgacggtc tgctggtagc gcaacgagcc cggaaacttg
      901 tcgggtgtca ggtagttata gaacgggttg tcgaccctcg tgtccagctc ggatttgaac
      961 tgataggaca gagccgggtc catcatgttg ttctgctcgc cctgaaccgc gttgccccac
     1021 actgtggaat tcggcagatt ctggcccagg ttcgtaaaga acgtcacgtc caggcggatc
     1081 cgccgcggca gttcgcgctg gacactgagg ttgatgcggt tattgatcgg ctgtttcgga
     1141 tactggttgt agtaggtgat agcgttgcca agctgatggt agcgcccgta ggatttcgcc
     1201 atcggcgcga gcaccgggtt cgccaccggg aacggatcgc tcagcaccgt ccgcggtacg
     1261 ccttgcagtg gggccagtgc atcggtcctc tgactgaaac cggcgttcgg caggttccag
     1321 ttcggaccca aagacgtctg gaacgggatg ctgtaccgcg agtagccaaa gcgcacactg
     1381 gtcttgtcgt cgatccggta ggccgcaccg aaacggggca ggaaaacgtc cttgggtgcg
     1441 ttgaacacgc ggggattatc gctgtccagg aactggtaag cgccggtcag gcgagccggg
     1501 aaccctccgt atttcgccag atccgggaag gtcaccgggt tggtctggaa ctccggaatc
     1561 ggatcgctca agtcgagata ccgtcccagg ttcccgtcgt gctccctcgg cgcctggtcg
     1621 tactcccacc ggatgccgag gttgagcgtc aggttccggc ttaccttcca gtcgtcctgg
     1681 aaaaagagcc cgatgttatt ggagatggtc caaatgggcg ccgcgtagtt ggtatagccg
     1741 ctattcacgt gtcccagcag gaaagaggcc catcccgcac cgctgacatc ggtaggggga
     1801 ttgatatagg tggaggcggt ggtggctccg gtgaaggcga acgtgcccca gttgggcaga
     1861 ccgctattgg atgtgtagtg ggagagcttg gcgccgaact tgagatagtg gctggtggtc
     1921 tgcttcatcg ccgtgccgga gtagttgaac tggcgcggca gcaggttcca accgctggcg
     1981 ttgccgaaag cagcgccatc caccgagaac gccgggaagt aaatcaccgg cgattgagac
     2041 agcgactgcc tccaccattc gttcggccag atggacttcc aaaagtcggt atccaccttg
     2101 ccgatgggga tatccagttc gtcggccgac cggatgtagc cgaagcggaa gttcaccagc
     2161 cacgtcgggc tgagcattcc gtccacgttg ccggccaggt tgagggcgta cattctggcg
     2221 gcgctccagc tcgagtaagc cgggctggat tcgagcgcct cgggaatctg ggtctgattt
     2281 tgccggctgt accgtccgaa cgcccgccag gccgtgttga actggtagtc gccgcggacc
     2341 gagaggttat tgtatgggtt cccgactctg gggctggctt tccagttgtt gagatgagtg
     2401 aagggttcgc ccgggttgtt gggttgatac acataggaca tcagcgcctt cgccgacgga
     2461 tcaatcaggc tgcccgggat tctgttgccc gggaacggcg tgcgcctggc ggtattggtg
     2521 gccggatcga gaaccgtggt catcgggtcg tagatcgtcc gcagattgcc gaactggttc
     2581 aggctttgtg aaaagtcgcc ctgtctctcg agatccgtgg gcagggtcat ctggctgagg
     2641 ccggagaccg tcgacgagtc ccacttctcg tagctgacga agaaaaacgc cttgtttctc
     2701 ccgtcgaata tcttcgggat ccacaccggg ccgcctacga ctccgccgcc gatgtgattt
     2761 ctggccttgt tcggcgtgcg ggtaatggcg ctcggcacgg cgttgaagta aggattacgg
     2821 ccgtaatagt agccggtgcc gtgcagttcg ttggtgcccg acttcatggc aatgctgatg
     2881 gctccgccgg cgctgttgcc gtattcggcg tccaccgggt tctgttgaac caccacttcc
     2941 tgaacggcgt ccatcgacgg cgcgtacgac ccgcgctgct cgacacgcgt atccgtacca
     3001 tccagcagca actggatacg gccgccggta gccccgccga tatccatacc cgcatggctg
     3061 atctggtaaa aggggaggct gttcgcggcg ttattgtacc ggttcaccac cgccggattc
     3121 agaacggcca gattgaatgg gtttcgcgca atcaccggca tctggtcgag catttttccg
     3181 atgacggtct ggctcatgga gctgttgttg aactcgaccg tggtggcgtc ggccgttacc
     3241 tcgatagtct ccgtcatcga gcccacctga agcgatgcat tcaccgtgac gtcgccgcgc
     3301 gtctgaacga tgatgccctt ctgttcaaac gcattgaagc cggccgcccg cacggcgatg
     3361 gagtaggcgc cgggctgcac caggtcaaac aaatacatac cgttttccgt cgtttgccgc
     3421 gtaacggcga tgccggtccc ctgattggtc agggtgacgt cagctccggt tacggcggcg
     3481 ttggacgggt ctgtgacgaa tccttgcact ctgccgcggt attcctgagc gcctagcggc
     3541 aggacacacc atgccaccag aagtccgaat aacgcgccac gtgcgcctgg ttttctactc
     3601 atcatgacct cccgtttcaa tagagaataa gcgatacccg taataaaccc tggaggaagt
     3661 ctattcggac ggatcgtcaa tgtcaacaga gttgcactac catcgggtaa gaaattctag
     3721 aatgagaggg actatggtaa cggcattcgt tttgatcaac acggcgcaag gacgcacgcc
     3781 ggacatcgcg caggcgctga tcgatcttcc gggcatcacc gaggcgtatt ccgtcgccgg
     3841 accatatgac ctcgtagcag tcgcgcgcgt gcggcatcat gaagagctag ccaacctggt
     3901 ggcgggacgc gtccagaaga tcccgggcat cgaaacaacg aataccctga tcgcctttca
     3961 ggcatacagc cagcgcgacc tggaagcgat gtgggacatc gggaacacgg agccggctgg
     4021 ataacagccg cttgctcacg cgcgcggctc cgtaacgcct ttgtttctga gccgcgacgg
     4081 tcacggagcg gttgttcgca tctcaattgt gataatgcag gctctcgatc aggttcggat
     4141 cgcgtacggg caccggcgcc gaggatgaga acgcgcgcgt gaaggccagc atgaacacac
     4201 ccagcgcgcc cagtccaagc gctgcttccc agatgccgaa tgccggtttc tccgagacgg
     4261 ccgggacgat catcaggtac agatcgagcc agcgtccggc cagtacgaca accgctacct
     4321 tagccaggat gcccgggttt ctcttcgtcg atttcggaag cagcaccagg aacggaatcg
     4381 cccagttcag cagcatgttc aatagaaaca ggggcgtcca taacccctgc tggcgccgca
     4441 cgtaatacac cgcctcttcg gtgatgttcg cgtaccacac cagcatgtac tggctgaacc
     4501 agatatacat ccagaacgtg ctgaaggcga aaagcagttt gcccaggtcg tgcaggtgtt
     4561 cctggttcac gaaatcccgc agcggaccgt ggttgcgcag ccggatcacc agcagaataa
     4621 tcaccgccag tcccgaggag aacagccccg cgaaattgta gacgccgaag atcgtgctgg
     4681 cccagtgggg ttcgagcgcc atgatccagt cgaagctggc gagcgaaaac gtcaccgcga
     4741 aggcgaacat gaaaatcacg gatagccgcg tgttccggcg cgagaaaaca aggtctccat
     4801 cggcatcctg ccgccgcgaa ttccggatca ccgcccaggc aaagcccatc cagactgcaa
     4861 gataaatgac cgcgcgggcg acaaagaatg gcaggtccag ccacagcgcc ttgaacccgg
     4921 caggttgctc atgcgtccac ggataaagcc aggagccgag gataaagaac aggatcagtc
     4981 cggcggcgcc ggcaggcagc gcggacgacg ccgcttcggg aacgcgccgc aacgccacgc
     5041 tccaacccgc ccccgtaacg tactcgagtg cgacgaagaa cgccgccgcc aggccgatgc
     5101 ccagcaggaa atagctgacc agcagaaggt tcgacgacat tcgcgcgggt gccagaaacg
     5161 ttcccgcgac aatcgccatc gcgccgatgc cggcgaacgc gcggaacagc cgcatcgatc
     5221 ccgccggctg catacccgta ttcatggacg cccctcctgt tgcgcaccgg cggcggcgcg
     5281 ctgcatgctt cgaatgtagg caatcacctg gcgccggtcc tcgggctcga tctgcgccgc
     5341 atagccgggc atgttcttga acccggcaga gacaatctcg aacatctttt catccgtcag
     5401 atccatggcg ttgggagcca gcagcgaagg cggtggagga aagccgcgca tagagaccgc
     5461 tccgtcgccc tttcccgttg ctccgtggca cggctggcag aacgtagcga atacagcctg
     5521 gccgcgttca ggagcgggtt tcgtcgcggg ccgttcctcg cgaaatggaa tcgtgccggg
     5581 caccggagcc tgaagcgtct tgccgtcggc aaagttttcg ttcgctgaaa atgaatcgta
     5641 agcggccgtc cgcatcatgt ccggaatgaa ttcgtaattc ggcttttccg gatgggagcg
     5701 agccatgtag gtcgcgccgg tcagaaccac cagcgcggcc agtagaaagg cattcaggcg
     5761 attcataccg gcgatccttc caactcgcgc tcctctacgt gcacgacgtt aaacttttcg
     5821 agcgcctcct gaacccgcac cggatcgaac agcgcatcgg tttcctcaat caccacggcg
     5881 aagcgatcgt ccgtcactcg cggatgaacc ggcttgactt tccggcccgg ccggaggcgg
     5941 ctgaccagaa agaacgcgaa caccgtgctc aagccggcga acagcaccat cacttcaaag
     6001 gtgatgggaa cgaaagcggg aagcgaatcc catggcttgc ctcccacgtt tacgggccaa
     6061 ttgtaagcgg tggtccagta ttcgaaccag actttcgcgg cggcgccgaa tagtcccagc
     6121 aggaaacaga cccacggcag gcgcgacggt tgaagcccca tcgctttgtc cagcccgtgc
     6181 accgcatagg gcgcgtacac atcgacgatc ttcaggccct gctcgcgcac ggcacgcgtg
     6241 gcgcccagca catcctcttc gtcgtcgaag atgccgatca gcactctacg attcatgccg
     6301 tcctcctccg ttactacggc cgtaactcaa gactcctttc acttcgccga ttgcaatcac
     6361 cggaagcagc cggcagaaca gcaggaagca ggtgaagaac aggccaaaac tgcctagcag
     6421 cgtagccact tcaatgacgg tcggccggta ccccgtccag ttcgaaggca gataatcgcg
     6481 atgcagggaa gtgacgatga tgacaaaccg ctcaaaccac attccgacgt tcaccagcag
     6541 cgacaccaca aaaaccgctc ccatatgggt gcgcactttc cggaaccaga gcaacagggg
     6601 aacggcgacg ttgcaggtga ccatgatcca ataagcccat gcaaacggcc ccatcgcgcg
     6661 gttgacgaaa acaaaccgct cgtattcgtt gcccgagtaa atcgcgataa aaaactccgt
     6721 cgcgtaggcg agacccacca taccgctggt ggcgatcacc agcttgcaca tggtctcgac
     6781 gtgccgcgcc gtgatgtaat cctccaggtg catggtcttt cgcgccacaa tcattagcgt
     6841 cagcaccatt gccatgccgg agaaaatggc acccgcgacg aaataaggcg gaaaaatcgt
     6901 ggcatgccag cccgggatta cggaagtcgc gaagtcccag ctcacaatcg tgtgcacgga
     6961 cagcaccagc ggcgtagcca gccccgccag tagcatataa acggtttcgt aacgctgcca
     7021 cgtacgatag gaaccgtccc atcccaggct gaagaatgaa tagatccgct tgcgcagtcc
     7081 ggatttcaac cggtcgcgca acgtcgccag atccgggatc aggcccaggt accagaaggt
     7141 ggccgagatg atgaaatacg tcgagatggc aaacgcgtcc caggcgagcg gcgatcggaa
     7201 attgatccac agcggcccgc ggaagttcgg atacggaaac acccagtagg caaaccacgg
     7261 gcgccccatg tgaatcagcg gaaagatgcc ggcgcacatt accgcaaaca gcgtcatcgc
     7321 ttccgcggag cggttcaccg aagtgcgcca gcgctggcgc agcagaaaca acacggcgga
     7381 gatcaacgtt ccggcgtgtc cgattcccac ccagaacacg aaatttgtga tgtcgaacgc
     7441 ccagccaacc gtgttgttga gaccccacgt gccaatgccc gtagccacct ggtacgacac
     7501 cgcggccaca cccaccagca acatggagac agccagcaga aagcccgccc accacagcgc
     7561 gccgggacgg cggtccatgg cggagcacac gtcttcggtg acgttcgaca ggcgcttgtt
     7621 gccctcgacg agaggagtcc ggaggaccga ataaacatta gccatggcgc tcttcctcca
     7681 cgccggacgc gcggaccagt ttcaggtaat gcacggacgg cttcagattg atctcttcga
     7741 gcacggtgta ttcccgattg ccccgcacca gccgcgacac tgcgctgtcc gggtcgttct
     7801 tgtctccgaa ggcgatggcc cgcgccgggc acgactgctg gcacgcggtc tcaaaatccc
     7861 cgtcgcgcag cggctcgccc cgccgcttcg cctcgaactt ggcgtcctga atacgctgca
     7921 cgcaaaacgt gcacttctcc atgaccccgc gcgaacggac ggtcacgtcg gggttcagca
     7981 ccatgttcgc cagcttgtct tcgcgcggat aatcgaacca gttgaaccgc cgggtcttgt
     8041 aagcgcagtt attcgcgcaa tagcgggttc ccacgcagcg gttgtagatt tgctgattga
     8101 gcccctcgga gctgtggacc gtggccagca ccgggcagac ggtctcgcag cccgcatgct
     8161 cgcaatgctg gcacatcatc ggctgatgag cgacggtgac gtcctctccg tcgcccgagt
     8221 agtagcggtc gatgcgaatc cagtgcattt cgcgcttgcg ccgcacctcg tcctttccca
     8281 caacgggaat gttgttctcc acctggcagg caatcacgca ggcggagcag ccggtacagg
     8341 cgttcagatc gaccgccatc ccccagtggc gtccgtgata agcgtggtcc gggggccaca
     8401 ggtctccatg cgccgcgtgc gcatgcggtt cgttatgcgc caggacctcc agcgtggttt
     8461 cctgcacaat gggccgcacg cctccggaag cgggagcgag gttgggcggc ggcgccacgg
     8521 tgtgatgact ttgcgtaatc gccagctcct gccgtttgcc cgtcttcttc acctggagcc
     8581 cacccgattc gaaccgcaga acaccgccgg cgaagcttgc caggtgcgcc gcgtttttgc
     8641 cgaccaggcc gttttcgccc gtactcgatc cggcggaaaa ccagcgcggg ccgacggaag
     8701 aaaagcgagc cgtttccgtg cggccatagc ccagcgcgac cgcaatcacg cgctcgtgct
     8761 gtccgggttg tataacggca ggaagctcca gagtgcggcc attccgttcg agctgcagaa
     8821 catctccgtc ccgcacatcc agcgtctgcg ccagcgccgg ggcgatggac gcatagttgt
     8881 cccacgtcgc tttcgtaatg ggatcgggca actcctgcag ccacgggttg taagcgtgtt
     8941 ttccgtcgag catcgaaggc ttgggatata ggacgaggtg cagaccgtca ggcgtggctt
     9001 cctgttgacg cggcgccgga ttcgccgcac cggcgatcca gcgcgacggg gattctttcc
     9061 ggtccacgcg cgcgaagccc tcgcgtagcc cgtcttccca attgaggcgg ccctgccact
     9121 gttcgcgcac caggtctctc gcgcttcgag gggacccggt ccatgccgac aggctttcgg
     9181 ccaccgtgcg cgcctcggac agcggttgta tggcgggctg gccgatgctg acaatgccgg
     9241 ccaccggttc ggcatcgctc cacgtttcga ggaaatgcgg aaccggacat actacatgtg
     9301 ccttcgatgc cgtctcgtct acccgctcgg tgaagctcac caccagcggc agtttctcca
     9361 gttgcgccgc cagcgccgcg ccatccggca gatcgtagac cggattcact ccatgaagga
     9421 acagggcgga gactttgttt gcggcaatct cgtcgagcag ggagtgcaac tcgccatcgt
     9481 tgccctgccg ctgccgcgaa gggcgcgcga tgtcgagcgt gctgccatag tttccgagct
     9541 cctggttgat gcggttcaca agtgtctgtg cttccacatc ctgcgttccg cacaccacca
     9601 ggctgcggcc gcgcgcggcc aatagccgcg acgcgaccgc atccgcgcga tctcccgtta
     9661 cgacagcggt gagcagcgcg cgaagccgtg ggaccgtttc atccggcgct atacgaattc
     9721 gccggccggc cttcgacccg gtcaccgaca accgggattc gatctgaata tgatatgaca
     9781 ccggacggct gccgcggtgc tcgggattcc ggcgctcgga gtgagctcgt gtaaattcca
     9841 ccggcgagat ccacgtaccc agaaaatccg catcgaaact gacgacgaca tcggcgttct
     9901 caaagcggta gtgcggaaga atgcgggtgc catgcgtaac ctggtgcgca tccagaatcg
     9961 ccgacgacga caacgcatcg tacgccacgt gccttgaatc cttgaagccc gcgagaaatc
    10021 gcccgatggc ggaaagcgta gtaggactgg ttaccgactg ggagaggaag cgaaccgcac
    10081 cgccgcgttg gcgaattgca tccaggcggg cagctacctc acggtccgct tcctcccaac
    10141 ttgcctgttt cccgcggagg atcgggtgtt tcaggcgaag actgtcgtac aaccccaaaa
    10201 tagatgcctg tcccacggcg cacaagccgc cgcgggagag gggatggcgt gggttgccct
    10261 caagcttgat gggacgccca tcccgggtct tgacaagaat accgcagccc gcggtgcagg
    10321 cgccgcaggt ggacgcatag taaataggac gcccggcgac ggctccttcg ggttgcacca
    10381 ggtgaggcat cgccttctgc tccggcgcac ggctgcatcc ggccagaaac gctcccgcga
    10441 aagtgaagcc cgcggccttg aggaaggcgc ggcggcccgc gtcgggttca aggatggaaa
    10501 gcggagtgga agcgaactcc gccccgcccg cttccgaagc cggcacaccc gcttcgcgct
    10561 cttcgagact ccgccaatat tgcaattcct cgggcatctt gctgttcctc atcagaagtg
    10621 gcaggctgag cagtcgatgg acgcctgcac cggctttccg gcgactcccg tccggtttgc
    10681 gttgcggtgg cagttgacac accagcccat gctgagatcg ggcacctgcc gtactcgctc
    10741 catggtttcc accgggccat ggcattcctg gcacgtgacc ccggcgttga cgtgcgggcg
    10801 atggtcgaag tagacgaaat cgggaaggtg gtggaccttc gtccaggcga tgggctgcgt
    10861 ctgtttcgcg gggtcccgct ccattttggc gttcagcgcc agcgcgtcgt agagcttctg
    10921 aagttcggcg gatacaatcg cgcgaggcgg ccgtttctcg ctcttggcca gttcatcttc
    10981 ctcgcgcaac gcgccgagcg gagcggtcac aaagcgatgg cagttcatgc aaacgctggc
    11041 cgccggtatg ccggcatggc ggcttttctc cgcgccgaaa tggcaatact ggcacgcgat
    11101 ctgaagctcg cccgcgtgca ggcgatgcga gtacgcaatg ggctgcaccg gctcatagtc
    11161 ctgctggttt tccggcaggt ggaaactgga agtgcgccag ccaagcagca tgagcgtgta
    11221 ggccagcccg ataagcagaa ccagcgtgac gatccgtgcg ctcatgccgc actccgctcc
    11281 gtgctcagct ttgccggcga ttccacgcgg gcgtacttca ggatccagac catggtcagc
    11341 gacaccagcg tgagaaaaac gaagacgatg aagccgatat ggtatccggg gcgtccaaga
    11401 tcgcggaccg caaacgccat gatgggcgga atgacgaatc ctccgaacgc gcccagtcca
    11461 cctacccagc ccgcggcccc gccgacagct tgcggtaccg cttgcggcac cagcttgaat
    11521 acggccgcgt tcgagatgcc catgccgatg gcaagaagca gttctccggg cagcgccagc
    11581 tcaaattgat tggagccggt caggatgaga gagccgacca gcatgatgaa cagcgccaga
    11641 atggccgtgt tctcgccgcc ttcgcgcaac tggtctgaaa ggattcctcc ggcgatgcgg
    11701 atgagcgacg tcagaatgga gaacgcggcc gtcagcaggc cggcggtgat ggggctcacg
    11761 tcgaagttcc cgcgccagta ggtgggcagc catgcggtca acgcgatgaa gccgccgaac
    11821 gtggtgaaat atacgagcac aagcgcccac gtgcgccaca caccggcgga gattcgaaga
    11881 ctgtctttca ggcttcccgc ggggaagagc gcctgcccgc gttccatggc ctgtttgcgg
    11941 gcctcgtccg gagacaggcc ccgttcacgc agttggaaat accaggcatt gcggccaagc
    12001 agcgcataca acacggttcc cgaaaccagc agcccaagcc agatcaggta cgagccagcg
    12061 agccccgcgg cggcgatggc aagcggcaac agaaaggtaa atagaccggg cgccaggttt
    12121 ccgacacccg cgaagacggc cagtgcgcga ccttgcgtct gctgcgtata ccagtaggag
    12181 acctgcccga tgccgggtga gaaggtggcg atgccgcagc cgctcaataa cgccagcaga
    12241 agaatcagcg gatacagaga cgcatccagc cgctgcggat acaggaagta aaccaccgct
    12301 gccagcccgc tcatgccggc gatggagagc gagaggagaa cgagaaacgg tttgcggcct
    12361 ccgttggtgt ccacccaggc cgcaaacgga atgcggagca gcgatccgga taaagcgggc
    12421 atcgctacca gaaagccgac agccagcggg ctcaactgca tcacttcctg aaatcgcgtg
    12481 gccgtgggac cgaacagggc aacggccgcg aaaccaatga aaaagcctaa ggtggcgccg
    12541 aagaggcctt gtgccggtgt gccgcgcagc ttgttcattg tgcccttcca gcttccggct
    12601 tgccgtacca gcgcaccact tgcggcttac gccacaggta cgggttggga accaccagca
    12661 cgtggaccag cctggtgaac gggaagaacc cgatcagcag ccatgcgttg acgatgtgca
    12721 acttcaccat gaacggcatc ggggcgatat aggcgacgtt cggattcagt ccggcgatcg
    12781 accacaggta aggcacggcc gagctcgcaa accaggaaga gccccagggg tgaaacaacg
    12841 cgacggccac gccggagccc acctgcacga gcagcagcac gaagagaagc cagtcggcgc
    12901 gcgaggttac ctgctttacc ttgctcaccg aaaaccggcg catgatacag gccgccaggc
    12961 cggccagggt caacagtccg aagatgagag ccgtcacttc gagaacgtag agccggagcg
    13021 gcttgctgtt ccatgccagc acctcgcgcg gaatcaggaa gccgataatg tgccccgcga
    13081 gaatgacaag aatcccgtag tgaaatggca cgagccccca gaaatgctgc tggttttcga
    13141 ggaactgcga cgacaaactg gaataactgt agcggcgcat ccggtaccgc tgtatcgtca
    13201 ccaggaaaaa cgtaaacagc gctacgtagg gcagcgcgac gaacagcaac tgatcgagat
    13261 aagtccatgc gctagtcatg accgtttccc tccgcgccca ccaaagacgg ctcaggcgca
    13321 tagagccgcg ccacggcggc caccaggttt tcgaaaatat tccccttcag tcccgaaagg
    13381 attttggtca gcgccggggc caggtattcc gttgtgaagg ccgcacgctc ttccgtgtcc
    13441 atcctgtcga tcagcggaat caaatgcgag aggtggtccg gcagctcggc ggattcttcc
    13501 acttcgtaac gccgcagggc acgccgcgcg gtgaccagaa actccccgcg ctcgtactgt
    13561 tcgccgtaga ggtgccagcc gatgtcgagt gtcgtgtccg ggttcatttc aaacgtttga
    13621 acgaacttct cctgcacgtc cgttgtggag agcggttcga gagcggcggc aaaaacaacg
    13681 agcggcgcgg cggcgggtgt tccggtggcg tcccgggcac aagccgccac gagcgccttg
    13741 tagtcttctt ccggatagca gaacaacgct cgcaaactca tgcgcccctc ctgggtccgg
    13801 tcaggaaacc gaagccggcg ttctgcttat gctccagtgg atcgcgcatc atctcgatcg
    13861 cctcttcacg gtgcgaaggc ggaatgacga accgttcttc aaacgtgcaa agggaggtga
    13921 gcttgtaaat cgcttccgct tcctccaccg tgctgccggc ttcccgcagc atccgctcgg
    13981 cggtcgccat atccacgtcg ccgaccgtga cggcacgccg gtaccagcgc acggcttttt
    14041 gcttccggag cgcgtatcgc acctttccct cgtggcccgc cccgaacagg ctggcgagaa
    14101 acttcatggg gatgcgcgct tcgtcgatat cgtgaaacag attttccgaa acgtggttca
    14161 ccgtggctcc ctctttggtg gccatcacgg gagccatcgg cggcacgtag aagagcatgg
    14221 ggagcgtgcg gtactcgatg tgaggaggca gcgcgatacc ccagaccttc acgaactggt
    14281 acaccggcga tttctgcgcg gcttcgatca ccgaagaatg cacgccgttt gcttgcgcac
    14341 cggcaatcac ctcgggatcg aacgggtcga gaatgatgga tcgctgcgct tctaccaact
    14401 gctcatccgg cagcttggcg atttcctcga tgcggctggc gtcatacaga agaacaccga
    14461 gataacggat gcgcccgacg caggaatgga agcaggccgg cgcctgtcct gtctctattc
    14521 gggggaagca caggatgcac ttttccgatt tgcccgtgaa ccagttgaag aagactttct
    14581 tataaggaca ggcggaaaca caagcccgcc aggcgcggca gcggttctgg tcgattaaaa
    14641 cgatgccgtc ctcgccgcgt ttgtacaatg cgccggacgg gcacgaagcg acgcacgagg
    14701 ggttcaggca gtgattgcag atgcggggga aatagaagaa caccagccgc tccacggcaa
    14761 acaactgctg ccgctgctcc ggcgtgagtc cttcgaggtt gcggtcgttc tccgcgtaga
    14821 cgggagagcc gccgagatcg tcatcccagt tcggcccgga ttcgatgttg atcaactcgc
    14881 cggtcaccat cgagatgggg cgcgcggtgg gctggtcggg tccttcgggc gcgtcgaaaa
    14941 gctccttgta gtcgtaggtc catggctcgt agtagtcgtc catggtgggc atgtgcggat
    15001 tgtggaagat gttgaagacc gtcttctgct tgctggtgga gcgcagttgg agctttcctt
    15061 cggccacttc ccagccgccg cggtactttt cctgatcttc ccaggccgtg gggaagccgg
    15121 tgcccggctt cgtttccaca ttgttccacc acatgtattc ggtgcctttg cgatcggtcc
    15181 agatattctt gcaggcgatg ctgcacgtgt ggcacccgat gcacttgtcc aggtggaaca
    15241 ccatcgagat ttgggatcgg acgttcattt gagatctccc ctctatcacc acttcagatc
    15301 aggaagtttt cgcacgagca catgcgtgtc gcggttgcag cctgtgggtc cccagtagtt
    15361 gaagtggaag gtgaactggc cgtaaccgcc caccatcagg ttcggcttca aacggacgcg
    15421 ggtgaggctg ttatgcccac ctgcccggcg atagttgcgc aaaggcgact tgggaaccga
    15481 gtaggtgcgc tccggcgaat ggtactggat acaaatggag cgcggaatcc gcgcgctgac
    15541 cgcggcgcgc gtcactacca cgccgttgtc gttgtggacc tcgacccagt cgttatccac
    15601 gatgccgagc tcttcggcgt ctttgtcatt catccagaac ggttccacac cgcgcgaaag
    15661 cgtcgtcatg cgctggttgt cgccgtaggt ggagtgaata tgccacttgc cgtgcggcgt
    15721 caggtagttc agcatcatcg tttttcccac tctgtcgctg aactgcagat cgccatactg
    15781 gacgggcaac ggtttcggtt tgaatgtcgg caggtgttcg ccgaagtcga tatagccggg
    15841 gtggtcgagg tagaaatgct ggcggcctgt aagggtgcgc cacggaacca ggcactccac
    15901 gttataggta aatgccgagt agggacgccc gccttcaacg aggccggacc acatggggct
    15961 gttcacaaac cggtgtggcc gcgattgcag gtctttataa ccggcgcgga cgctgcggct
    16021 cttttccgcg agatgagcca gaggaagtcc caccttccgc tccatattct tgtaggagcg
    16081 gtaggccagc tcaccattgg tgaccgtggc gaagcgcagg atggtatcgc agacctgttc
    16141 gtcttcgaat aacgaaggat agctgccgcc gttccaactg acgacagggc cgcgctcgcg
    16201 cgcttcttcg tagaggtcgc gcacttcgta atgcgtgccg tgcgcgccaa ggccgttttc
    16261 cgccactttc ggaccgaacg agatgtattg atcgtacaga tggcggtaat cgcggctcac
    16321 cactttgagc gccggcatcg tctttccggg aatggcttcc acctctccgg acatccagtc
    16381 gccgatcttt gtctgcgcaa tttccgcggc gctgtcgtgc gccagcggcg tggcgacgag
    16441 atcctggacc ggctcgggga agtgtcgcgc cgcaagctcg gagaacttct ttgcgatggt
    16501 ccggaaaatc tgccagtcgg tcttcgattc ccagcagggc ggcaccgcag ccgacagcgg
    16561 atgaatgaag ctgtgcatgt cggtggaatt gagatcggcc ttttcgtacc aggtcgccgc
    16621 cgggagcacg atgtcggaat agagtgccga ggtgtccatg cggaaattca ggtcgacgac
    16681 gaggtccatc tttccctgcg gcgcgttttc gtgccaggcg atctggttga ccgatcccgc
    16741 tgccacctct tcggaaatcg cgttggtgtg cgtgccgaga tagtgcttca gaaagaactc
    16801 gtgccctttc gcactcgaca tgagcgcgtt gccgcgccag atgaaccaga cgcgaggcca
    16861 gttctccggt gcgtccgggt cctccacggc gaacttcaga ttgcggctct tcagttgatc
    16921 caccgtatag cgaacgacgt cgtcagccga ctgtgccccg gctgcttcgg cgtccttcac
    16981 gatcgcgagc gaactccggt cgaactgcgg atagaacgga agccagccgt atcgaaccgc
    17041 ttccacctgg cggtccgcgg tgtggccgtg cgcgaaggag ccgggccgcg cgtgctgcgg
    17101 cacgttgtga tagtcggtga attccttctc gtagcgccac tggttggtgt ggatgtaatg
    17161 ccagctcggc gcgttttgca gacgcgacgg cggataccag tccttgccga atgcgatgga
    17221 ggaccaggac tcgccgggag ccagcttttc ctgcccgacg tagtgcgcga ggccgccgcc
    17281 gttcacgccg atgcagccgc agaacatcag cgcatacatg ccggcccggt acatcaggtt
    17341 gttgtgatac cagtggttga ttcccgcacc gatgatgatg gtgcactttc cgttagtgag
    17401 ccgcgcggtg ttgccccact cgcgcgcaaa ccggatcacc atctgacggt ccatgcccgt
    17461 atacttttcc gaccacgccg gcgtgtaggg agcctgttcg tcgtcgtagc tttccgggta
    17521 agcgccttcg aggccgcgtg ggactccgaa ctgcgccatc atcaggtcgt agaccgtggc
    17581 gacggtaacc acctcgcccg a
//
LOCUS       DASTMK010000257         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_268274, whole genome shotgun sequence.
ACCESSION   DASTMK010000257 DASTMK010000000
VERSION     DASTMK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268274"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFQ79_11105"
     CDS             <1..550
                     /locus_tag="VFQ79_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0140255.1"
                     /translation="DESVPDDCGSAVLPAEHPVDIEVLVSGRVLVVEDNLINQRVAIR
                     LLEKAGLSVSVASNGSEALEQLDREFFDLVLMDVQMPEMNGYETTQAIRSREKAIREG
                     GMAVQPGSSFHAARRGDRAIPIIAITAHAMPSDWETCISAGMNDYLAKPIESRALLRV
                     VSKWLRLGGGSPPTISPGSISV"
     gene            560..2218
                     /locus_tag="VFQ79_11110"
     CDS             560..2218
                     /locus_tag="VFQ79_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0140256.1"
                     /translation="MPLHAEPNHAAGAFVRSQTQGPVVPLIEKTIAAAFDDAVARNPA
                     GTALVSCHQNVRLSYEELRDKVEALAAGLWSVGIRPGDRIGVWASNCLEWICLQFSAA
                     RTGAVLVNVNPAYRSHELRYVLARSRMKAIFLHEQDARVNYLQVLEESRHGQELALEH
                     VIVLGSDAWNALADERGPAPRPALTPEDVANIQYTSGTTGAPKGVLLTHRNLLNNAML
                     IGRQMQVSSADRLCSPVPLYHCFGCVMSSLMTMVNSAALVLPAAQFDATATLRAIHDE
                     RCTMLYGVPTMFIAELEHPDFESYDLTSLRTGIMAGAPCPVEVMQKVTGRMHCRDLTI
                     AYGQTESSPVITMSSLEDGIGLRVSTVGRVMPNTEVKVVSPDTGEAVPTGTMGELCTR
                     GYLVMKGYDGDPEGTAAAIDSEGWLHTGDIAIMRPDGYFHIRGRLKDTIIRGGENIYP
                     REIEEFLHQHPDIADVYVVGIPHERLGETVLAWIRLKNGARMTVADVRDFCRGRIAYF
                     KTPQYVRFVDEFPMTVTGKVQKYRIREIEIEERGLRAAQSIRTA"
     gene            complement(2226..3125)
                     /locus_tag="VFQ79_11115"
     CDS             complement(2226..3125)
                     /locus_tag="VFQ79_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140257.1"
                     /translation="MMRLNRRNALAALLAGPPAFTRQTATPHQVPFGSYRVSRLMVGG
                     NPVSGNSHVSAGMSREMLEYFTAANVKKLLRACEQAGVNTWQSRADRHILRLLSEYRQ
                     EGGRIQWIAQTASELVDIPRNIRDCTAAGAIGVYHHGSQTDRYWASGKIEQARETLKV
                     MRDAGVRVGLGTHIPEVIDYVEDKGWDVDFYMTCLYNLARSKEELAHLAGGATGELFL
                     DADRERMLERVRRTPKQCLVFKVYGASRKCGSRPQMLDALALAARYAKPSDCFVIGMF
                     PKHSEQVRENCELLTAALSAARS"
     gene            3173..4300
                     /locus_tag="VFQ79_11120"
     CDS             3173..4300
                     /locus_tag="VFQ79_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140258.1"
                     /translation="MKEDRRRFLAGGAAMLASARMKAQAEKELRTAMIGIGSRGGGLL
                     KQVLAQQNVKVAAVCDIDPQARDNAQTAAGRDNPRAYTDYRQVLDLKDVDAVVIATPC
                     DLHAEMSAAALEAGKYVYCEKPLGITPEQVKRALDASRRAKTFLQIGQQLRYFPGMRE
                     AVRLIQEEKILGAPFVIKAQRHSTPVPLEAQQKRPAWYKDVRRSGDLIVENAVHNLDV
                     CNWITGSRPTSAYGHGKKYLPEQKPAGTEMMDGFSVEYIYDNDMHLDYSQLYLHPRTL
                     KTLPNYQWYLVFGEKGTLDFTQGLFYDIQGTAEPREVVPQRIRERRENAMSEFYACIR
                     EGRKPVAGVEVGATAALTVIMGREAIYRRRMVTWKELGVEV"
     gene            complement(4310..>4619)
                     /locus_tag="VFQ79_11125"
     CDS             complement(4310..>4619)
                     /locus_tag="VFQ79_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140259.1"
                     /translation="VADGHRIWNLNTPRVPEGVDDAKVRRRLLESHGIEILGGFGPLA
                     GKIFRIGIMGPLATEESILSLLEALETALQAEGYKAKESGRAAAREAFADLDRKPQPA
                     "
BASE COUNT          884 a         1461 c         1453 g          821 t
ORIGIN      
        1 cgatgaatcg gtcccggacg actgcggctc cgcggttttg cccgcggaac acccggtgga
       61 catcgaggtt ttagtttccg ggcgtgtgct ggtcgtggag gataacctga tcaaccagcg
      121 ggtcgcgatc cggctgctcg aaaaggccgg cctttccgtg tcggtggctt cgaacgggag
      181 cgaagcgctg gaacaactcg accgcgagtt cttcgatctc gtcctgatgg acgtgcagat
      241 gccggagatg aacggatatg agaccaccca agccatccgt tcgcgcgaaa aggcaatccg
      301 ggagggcgga atggcggtgc agcccggttc ctcgtttcat gcagcgcggc gtggagaccg
      361 ggcaattcct atcatcgcga tcaccgccca tgcgatgccc agcgactggg agacttgcat
      421 ctctgccggc atgaacgact atttggccaa gcccatcgag agcagggctc tccttcgggt
      481 cgtctcgaaa tggctgcgcc tgggcggcgg cagcccgccc acgatttccc caggttcaat
      541 atctgtataa tcggcctttg tgcccctgca cgcggaaccg aatcacgccg ccggcgcctt
      601 tgtgcgcagc cagacccagg ggccggttgt acctcttatc gagaaaacaa tcgccgcggc
      661 tttcgatgat gccgtggcgc gcaatccggc gggcactgcc ctggtgtcgt gccaccagaa
      721 cgtccggctc tcctacgagg agctgagaga caaggtggaa gcgctcgcgg ccgggctatg
      781 gtccgtggga atccgtcccg gcgaccgcat cggagtctgg gcttcgaact gcctggaatg
      841 gatctgcctg caattcagcg cggcccggac cggcgccgtg ctggtgaacg tgaatcccgc
      901 ctatcgctcc cacgaattgc gctatgtcct ggcgcggtcg cgcatgaagg cgatcttcct
      961 gcatgagcag gatgcgcggg tcaactatct ccaggtgctg gaggaatcgc gccacggaca
     1021 ggaactcgcc ctggagcacg tcatcgttct cggcagtgac gcctggaacg cactcgcgga
     1081 cgaacgcggt ccagcgccgc gccctgctct aacaccggag gatgtggcga acatccaata
     1141 cacgtcgggt acgaccggtg ctccgaaggg tgtgctgctg acgcatcgca acctcctgaa
     1201 caacgcgatg ctgatcggcc gccagatgca ggtgtcctca gcggatcgcc tctgctcccc
     1261 ggttccgctc tatcactgct tcggatgcgt gatgagttcg ctgatgacca tggtgaactc
     1321 cgccgcgctt gttctgccgg cggcgcagtt tgatgccacg gccacgctgc gcgctatcca
     1381 cgacgagcgg tgcaccatgc tttacggcgt gcccacgatg tttatcgcgg agctcgagca
     1441 ccccgatttt gagagctacg acctcacctc gctgcgcacg ggaatcatgg cgggcgcgcc
     1501 gtgccccgtc gaagtgatgc agaaggtgac cggccggatg cattgccgcg acctgacgat
     1561 cgcctacggc cagacggaaa gctcgccggt catcacgatg tcgagccttg aggatggcat
     1621 cggcctgcgg gtcagcaccg ttggccgggt gatgcccaat accgaggtga aggtggtttc
     1681 gccggatacc ggcgaagccg tgcccaccgg gaccatgggc gagttgtgca cgcggggcta
     1741 tctcgtcatg aaaggctacg acggcgatcc ggaaggcacc gccgcggcca tcgacagcga
     1801 aggctggctg cacaccggcg acatcgccat catgcgtccg gacggctact ttcatatacg
     1861 gggcaggctg aaagatacga tcatccgcgg cggagagaac atctacccgc gcgagatcga
     1921 ggagtttctg caccagcatc ccgacattgc cgacgtgtac gtcgtgggca ttccacacga
     1981 gaggctgggc gagacagtgc tggcctggat tcgcctgaag aacggtgctc gcatgacggt
     2041 ggcggatgtc cgggactttt gccgcggcag gattgcctac ttcaaaacgc cgcagtatgt
     2101 ccgcttcgtg gacgaatttc ctatgacggt caccggaaaa gtgcagaagt accggatccg
     2161 cgaaatcgaa atcgaagaac gcggactccg cgccgctcag tccattcgta cagcctgagc
     2221 ccgggtcagg accgcgccgc gctgagtgcg gcggtaagca gctcacagtt ttcgcgcacc
     2281 tgctcggagt gcttcgggaa catcccgatc acgaaacaat cggacggttt cgcgtaccgt
     2341 gccgccagag ccagcgcatc cagcatttgc gggcgggatc cgcatttcct cgacgcgccg
     2401 tagaccttga agaccaggca ttgcttcggc gtgcggcgga cgcgttccag catccgctcg
     2461 cggtcggcat ccagaaacag ctccccggtg gcacctccgg ccaggtgcgc cagttcctct
     2521 ttcgaacgcg ccaggttgta gaggcaggtc atgtaaaaat cgacatccca gcctttgtct
     2581 tcgacataat cgatgacttc cggaatgtgc gtccccagac ccacgcgcac cccggcgtcg
     2641 cgcatcacct tcagcgtctc gcgcgcctgt tcgattttcc ccgatgccca atagcggtcc
     2701 gtctggctgc cgtggtggta aacgccgata gccccggcgg cagtgcagtc ccgtatgttt
     2761 cgtggaatgt ccaccagctc ggaggcggtc tgtgcgatcc attgaatgcg cccgccctcc
     2821 tgccggtatt cactcagaag ccggaggatg tgacgatccg ccctggactg ccaggtattg
     2881 acgcctgcct gttcgcacgc gcgcagcagt ttcttcacgt ttgccgccgt gaagtactcc
     2941 agcatctcac ggctcatccc ggcggataca tgcgagttgc cgctgaccgg gttccctccc
     3001 accatcagcc gcgaaacacg ataagatcca aaaggcacct gatggggcgt ggctgtctgc
     3061 ctggtaaagg cgggcgggcc cgcgagcagg gcggccagcg cgttgcgcct gttcaaacgc
     3121 atcatggacc tccggataac cgaaccttat tataccgatt aggagatcga ctatgaagga
     3181 agaccggcgg cgatttctgg cgggcggtgc cgccatgctc gcatcggccc ggatgaaggc
     3241 acaggcggag aaagaactcc gcacggcgat gattggaatc ggcagccgcg gcggcggtct
     3301 gctgaaacag gtgctcgccc agcagaacgt gaaagtggcg gccgtatgcg atatcgatcc
     3361 gcaggcgcgc gacaacgcgc aaacggccgc gggccgggac aatccgcgcg cctatacgga
     3421 ttaccgccag gtgctggacc tgaaggatgt ggacgcggtg gtgatcgcca cgccctgcga
     3481 cctgcatgcg gagatgtccg ccgcggcgct ggaagccggc aagtatgtct attgcgaaaa
     3541 gccgctgggg attacgccgg agcaggtgaa gcgcgcgctc gacgcctcgc gccgtgcaaa
     3601 aaccttcctg caaatcggcc agcaactgcg ctactttccc ggcatgcggg aggccgtccg
     3661 gctgattcag gaggagaaga tcctcggcgc tccgttcgtg atcaaagcgc agcggcacag
     3721 cacgcccgtg ccgctcgaag cccaacagaa gcgtccggcg tggtacaagg acgtgcgccg
     3781 ttcgggcgac ctgatcgtcg aaaacgcggt gcataacctg gacgtgtgca actggatcac
     3841 cgggagccgc cccaccagcg cctacgggca cggcaagaag tatctgccgg agcagaagcc
     3901 ggccggcacg gagatgatgg acggcttcag cgtcgagtac atctacgaca acgacatgca
     3961 cctcgactac agccagcttt acctgcaccc gcgcacgctg aagacgctgc cgaactacca
     4021 gtggtatctg gtattcggcg agaagggaac gctggatttt acacagggcc tgttctatga
     4081 catacaaggc acggccgaac cgagggaggt cgtcccccag aggatacgcg agcgaagaga
     4141 gaacgccatg agcgagttct acgcctgcat ccgcgaaggc cgcaaacccg tcgccggcgt
     4201 agaagtgggc gccaccgccg cgctgacggt gatcatgggc cgcgaagcga tctaccggcg
     4261 gcgcatggtg acgtggaaag aactcggcgt cgaagtctga gcccggctct caggcgggct
     4321 gcggcttccg gtcgaggtcg gcgaacgcct cccgcgccgc ggctctgccg ctttccttcg
     4381 ccttataacc ttccgcctgg agagcggtct ccaatgcctc cagcaaagac aaaatgctct
     4441 cttcggtggc cagcggcccc atgatgccga tgcggaagat ctttcccgcg agcggcccga
     4501 agcctcccag gatctcgatg ccgtgcgatt ccagcagccg cctccggacc ttcgcgtcgt
     4561 ccacaccctc cggcacgcgc ggcgtattca gattccagat gcgatggccg tcggctacg
//
LOCUS       DASTMK010000258         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_492711, whole genome shotgun sequence.
ACCESSION   DASTMK010000258 DASTMK010000000
VERSION     DASTMK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492711"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            655..921
                     /locus_tag="VFQ79_11130"
     CDS             655..921
                     /locus_tag="VFQ79_11130"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEU0140260.1"
                     /translation="MAASMNISLPETMKSFVDEQVRSGGYGTASEYIRELVRRDQKER
                     AEARLEALLLESLDSGEDIPVTPEFWETLRGKLAARRAKRKNSR"
     gene            1147..4242
                     /locus_tag="VFQ79_11135"
     CDS             1147..4242
                     /locus_tag="VFQ79_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0140261.1"
                     /translation="MKAPILCALFLALAGGLISTVRADVFYVATDGSDAAGNGSFERP
                     WATITAAVGRVPETGGLILVRDGVYTGRTRISRKFRDWLVLRAENPYRAKLQAAQEAP
                     LQIYDSAFVEVSGFDVSRSTPDTSAPLAAQIARSENIVLRNNIIHDSRNNDLLKVNES
                     PRNILIIGNVFYNPQGEAGQHIDLNGCTNAVVRDNIFFNDYAGSGAEDRKNSSAYIVI
                     KNSGNVAESRHFHVTSNIFLNWEGGIGKNFLLLGEDGKPFHETQDVLIENNLMIGNSQ
                     NQMRSAFGAKGVKDVVFRNNTIVGDLPSSAFAMRLNREVSNLVNRNLRFFNNVWSDPT
                     GTMRNFSDGRPDESDELEIRNNLYWNGGNDVPRGSVLSPSDDPEAMFQDPQLSPTEGL
                     VLPRWNETAFLSGNTTIRQEFERLVARYGTPAPSSPVYGRSLPDETPWTDILDRVRYN
                     RLDLGAVQVDAPAGPLRLLLISNELPGGLSSHLNQVILEYPAGPEGEVVELASTYPER
                     ASVPERIYIAPGAQAASFTIRTTRVDGLTSLWITATWNGITRPAELLLIPEGARGIDL
                     SPDSLISGTSSTRNLVLIDGVAPDEGLVVSLTSSHPDLVTVPETVSIAAGRSYSEFFP
                     VATKFTPEQTNVRIIATSGAGPASAILRVMPSPFRIALRSESLAAGSTLERNRIFIDR
                     AAPSGGATVYLSSSLPDVLIVPETVVVPEGQNIAEFPLRTGPVNTRTVGRITAVLDNS
                     RATVDCAVSPVVPSSILGSSSIGAGATTTFRVLLNSAAYEPVTIQLAAEGMDAALPEE
                     VTIPAGENTGSFPVTPALVAAPTKLRLFATYLNRTISLEVTVTPGRLYSFSVPATLWS
                     GESTEALIVLTGRAPLGGLDLRLSTNAPDLLVLPESVQVPEGATSVRFRFRANLFVES
                     RPNVTLTVSAADSSLERRLTLNAAELTLSFLQAATFTSGNNLTLDIRFKGRLPSGSTL
                     QLKSSNPDLIAVPESVSVPDGASFVRATVKTKAVTTDTEVTVTASYPGGSHSRAITLK
                     P"
     gene            complement(4316..>5560)
                     /locus_tag="VFQ79_11140"
     CDS             complement(4316..>5560)
                     /locus_tag="VFQ79_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0140262.1"
                     /translation="LDPSAPPQHIPPGVSVLLRVASDAVPVQPRNVSFLLVMYAEYNA
                     KEVRFVSFSPVLRAWVRRQPRLKRVLGGALRTAALGKHTLAAQFPILIQPRTRTMALA
                     ITAHCNLSCDGCRYGRDFMTGRQMTLPLIRQVLNDASGLGVYAVRLYGGEPLLHPDLP
                     AMVRHAWSAGIKPWVTTNGILLRQRIDGLYEAGLRQLTIGLYGTGADYDSYVQRKGAF
                     ALLEKGLEYVRDRYGMTVDIKLCFLLSRRTAEIAALRNAWEFTERLKAFFSVDLIHYS
                     LPYFTYGSDQSLEFRPEDASRIQAVAEELLEIHARRPDLYRESPASIRSIPDWLLRGS
                     GMRVPCDMYDFLWIGADGSVQLCYAHFPLGNVHERPLRDIAYQAAHKEAARKAFQLDC
                     PNCHCERETRIQKHLPSLRRYR"
BASE COUNT         1162 a         1672 c         1590 g         1136 t
ORIGIN      
        1 gcgaattgcg attttgtcta ggtaacaatt ggcaaaaatg gcaggaaatt tccgaaatgt
       61 cggccgccac agagttcgcg attggcagat aaccccggtc gaatctctcg aatgaacgcc
      121 tgcaggatct gtggattcat attcggcttt gtttcgactg ggcggtactc taggtgatac
      181 agggctggat atagggaacc gtgttcgacc tgaaggatcg ctccagatgt gatgtgcgct
      241 cgatgattgt cgcgattgta tggccgtggg ccggtccgac aggggttggg ggcgagttta
      301 cgttttcacc cgttgggtgt gggattgcca ggtagatgag tgtgcacccc cagcagcgga
      361 caagtgtgcg gtatttccgc aaacttcagg agaaatggga agtaaagtac cgtgatccag
      421 gcacttcctt tagtggataa ctcgtccatc taccgcgtgc gcgatattct tcgcggaaat
      481 ccgggacttg gccggtatgt ggcaccgagg atagtgaaga tcccacctga tatccgagaa
      541 attgtcgtac ggcaggcgtt ctaattgtcg tgatcagata atgccataaa ttgacaaatt
      601 ctgccatcga gccttatagt gatctgtaga cgacctcata caggagtttg cgccatggca
      661 gcctcgatga acatttccct gccggagacg atgaagtcct tcgtggatga gcaagtccgg
      721 agcggcgggt acggaacggc aagcgaatac atccgcgaac tggtgcgccg cgatcagaag
      781 gaacgcgcgg aagctcgcct ggaagcgctg ctgctcgaaa gcctggatag cggtgaggat
      841 atccccgtga cgccggaatt ttgggagacg ctacgcggca aactcgccgc ccgcagggcc
      901 aagcgaaaga attcgcgctg atgctgccgg tcatactcct tccggccgct gagaatgact
      961 tgctcgaaca gactgaatat ttaggatgct ggcggcgaag aggcttgtcg cgatcctgcg
     1021 aatcgtcgag gttagccctt ggcctggcgt gctagaatgt caatgattcc taagctgtaa
     1081 ccccgcgtcc cagagttttt ctatcctgcg ccagcctcgt gagcgcggct ccatctgaag
     1141 gattctatga aggctccaat tctttgcgcg ctctttctcg ccctggccgg cggactcatt
     1201 tccacggtcc gggcggacgt cttctatgtt gccaccgacg gcagcgatgc agccggaaac
     1261 ggctcgttcg agaggccctg ggccacgatt acagccgccg tcggaagagt acccgagacg
     1321 ggaggactca tcctcgtacg ggacggcgtc tacaccggcc ggacccgcat cagccgcaaa
     1381 tttagagact ggctggtgct gcgcgccgag aacccttacc gtgcaaagct ccaggcggca
     1441 caggaagctc cactgcaaat ctacgactcg gctttcgtgg aagtctccgg gttcgacgta
     1501 tcccggtcga cgcccgacac gagcgctccg ctggcagcgc aaatcgcccg gtccgaaaac
     1561 atcgtcctcc gcaataacat cattcacgac agccgcaaca acgacctgct gaaggtgaac
     1621 gagtcgccgc gaaatatcct catcatcggg aatgtcttct acaaccctca aggcgaggcg
     1681 gggcagcata tcgacttaaa cggctgtacg aacgccgtcg tacgggacaa catcttcttc
     1741 aacgactacg cgggcagcgg agccgaggac cgtaagaaca gcagcgccta catcgtcatc
     1801 aaaaattccg gcaacgtagc ggagagccgc cactttcacg tcaccagcaa cattttcctg
     1861 aattgggaag gtggtatagg caagaacttc cttctgctgg gcgaagatgg aaaaccgttt
     1921 catgagacgc aggacgtgct tatcgaaaac aatctcatga tcggcaactc ccagaaccag
     1981 atgcgttcgg ccttcggcgc caaaggcgtg aaagacgtcg ttttccggaa caacaccatc
     2041 gtaggcgatt tgccttcttc cgcttttgcg atgcggctga accgcgaagt gtcaaacctg
     2101 gtaaaccgca atctccgctt cttcaataat gtgtggtctg accccacggg gacgatgaga
     2161 aacttctccg acggccggcc ggatgagtcg gatgaactcg agatccgcaa taacctgtat
     2221 tggaacggcg gaaacgatgt gccgcgcggc tccgtgctca gtccctccga cgatccggaa
     2281 gcgatgtttc aggacccgca gctttcaccc acggaggggc ttgtactgcc ccgctggaat
     2341 gagaccgctt tcctcagcgg aaacacgaca atccgccagg agttcgagcg gctggtcgcg
     2401 cgctatggaa caccggcgcc gtccagtccc gtttatgggc gctctttgcc ggacgaaaca
     2461 ccgtggaccg atattctgga ccgggtccgt tataaccgtc tcgatctggg cgccgtgcag
     2521 gtagatgcgc cggccggccc cctgcgcctg ctgctcatct cgaatgagtt gccgggcggg
     2581 ctctcttccc acctgaacca ggtgatcctg gagtacccgg cggggccgga aggcgaggtg
     2641 gtggaactgg ccagcacgta cccggagcgg gcttccgttc cggagcggat ctatattgcg
     2701 ccgggcgcac aggccgccag tttcaccatt cgaaccaccc gcgtggatgg attgacttcc
     2761 ctgtggatca ccgccacctg gaacggaatc acccgccccg ctgagctgct tcttattccg
     2821 gaaggggccc gcggtatcga tctcagtccc gattctctga tctcgggcac atccagcacg
     2881 cgcaacctgg tattgatcga cggcgtcgcg ccggacgaag ggcttgttgt ttccttaacc
     2941 agttcgcatc ccgatttggt cacggtgccc gaaacggtct ctatcgccgc cgggcgttcc
     3001 tattccgaat ttttcccggt tgccacgaag ttcaccccgg agcaaaccaa cgtcagaatt
     3061 atcgccacgt cgggcgccgg gccggccagt gcaatcctgc gcgtaatgcc ttcgcccttc
     3121 cgcattgctc tccggtcgga atcgctggct gccggctcca cgctggagcg gaacagaatt
     3181 tttattgacc gcgctgcacc gtccggaggg gccaccgtct atctttcaag ctctctgccc
     3241 gatgtgttga ttgtgcccga aaccgtcgta gtgcccgaag ggcagaacat tgccgaattt
     3301 cccctgcgga ccggaccggt gaacacacgc accgtaggaa gaatcaccgc cgtgctggac
     3361 aacagccggg caacggtgga ctgcgccgtg agccccgtgg ttccaagctc catcctgggt
     3421 tcgtcttcga tcggcgccgg cgccactacc actttccggg ttttgctgaa cagcgccgcc
     3481 tatgagcctg tcaccatcca actggctgcc gaagggatgg acgcggctct gcccgaagaa
     3541 gtgactattc cggccggtga gaataccggc tccttcccgg tgacacccgc gctggtcgcg
     3601 gcacccacaa aattgcgcct cttcgctacc tatttgaata gaaccatttc gttggaagta
     3661 accgtcacac ccggcagact ctactcgttt tccgttccag ctacactctg gagcggcgag
     3721 tctaccgagg ccttgatcgt gctgacaggc cgtgctccgc tcggaggtct tgaccttcgg
     3781 ctgtctacga atgctccgga cctgttggtt ctgcccgaga gcgttcaggt tcccgaaggc
     3841 gcaaccagcg tccgattccg tttccgcgcg aatctgtttg tggaatcgcg ccctaacgtg
     3901 accctaaccg tctccgccgc cgactcttcc ctcgagcgcc gtctgactct caatgccgct
     3961 gaattgacac tgagtttctt acaagccgca acgtttacca gcggcaataa tctgacactg
     4021 gatatccggt tcaaaggaag gctgccttcc ggctcgacgc ttcaactgaa gagttccaat
     4081 ccggacctga ttgccgtacc cgaaagcgta tcggtcccgg atggcgcgtc gtttgtgcgg
     4141 gccacggtca aaacgaaggc ggtaaccacg gacacggagg taacggtcac ggcttcctac
     4201 cccggcggaa gccactcccg cgcgattacg ctgaagccct gagagggaga actcgccacc
     4261 tttcttcgtg caaccgtggt gcctgtgctt cgccaacccg gaaacacatc gtccatcatc
     4321 tatagcgccg caggctggga agatgcttct gaatgcgagt ttcccgctcg caatggcaat
     4381 tcgggcaatc gagctgaaac gccttccgcg ccgcctcttt gtgagccgcc tggtacgcaa
     4441 tatcgcgaag cggccgctcg tgaacgttcc cgagcgggaa gtgtgcatag cagagttgga
     4501 ccgagccatc cgcgccgatc cacaaaaagt cgtacatatc gcagggaacc cgcatgccgc
     4561 tgccgcgcag caaccagtcg ggaatggaac gtatactggc gggcgattcc ctgtacaagt
     4621 ccggtctccg cgcatggatt tccagtagct cttcggccac cgcttgaatc cgcgaggcat
     4681 cctccggccg gaactccagg gactgatcgg agccgtaggt aaaatacgga agtgagtagt
     4741 gaatcagatc gacgctgaag aaggccttca gccgttccgt gaattcccac gcatttcgca
     4801 acgctgcgat ttcagccgtg cggcgcgaga ggagaaagca gagcttaata tcgacggtca
     4861 tgccgtaccg gtcccggacg tactccagcc ccttttcgag cagtgcgaac gcacctttcc
     4921 gctggacgta ggaatcataa tcggcaccgg ttccatacaa cccgatcgtc aattgccgca
     4981 gtcccgcttc ataaagtcca tcgatgcgct gccgcaacag aatgccgttc gtcgtcaccc
     5041 acggctttat gccggcgctc caggcatgcc gcaccatcgc gggcagatcg gggtgcagta
     5101 acggttcacc accgtacagg cgaacggcat acacaccgag cccgctcgca tcgttcagga
     5161 cctgcctgat gagcggcaat gtcatctgcc ggcccgtcat gaagtcccgt ccgtaccggc
     5221 acccgtcgca cgacaggttg caatgcgctg taatggccag ggccatggtg cgtgtgcgcg
     5281 gctgaatcaa aatggggaat tgcgcagcaa gggtatgttt tcccaacgcc gccgtgcgga
     5341 gtgctccacc cagcacccgc ttcaggcgcg gctggcgtct gacccatgct cgtaaaaccg
     5401 gtgaaaagct cacaaagcgg acctctttag cgttgtactc agcatacatg actaaaagga
     5461 acgatacgtt gcggggctga actgggacgg cgtccgaagc aacgcggagc aggacactca
     5521 cgcctggcgg aatgtgttgc ggtggtgctg acggatccaa
//
LOCUS       DASTMK010000259         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_492718, whole genome shotgun sequence.
ACCESSION   DASTMK010000259 DASTMK010000000
VERSION     DASTMK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492718"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VFQ79_11145"
     CDS             <1..770
                     /locus_tag="VFQ79_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEU0140263.1"
                     /translation="LIGCKIAAANRLPASFFVSVAYDCWAFRRLGVRLDARDGAITEW
                     LYRDPERPVAKMAASTGFSLTGREVVLTPPLTEEAVRALKVGDVVLVTGEIFTGRDNV
                     HHHLMSHEPPVDLRGAIIYHCGPVMLKQGEEWTVKAAGPTTSSREEPYQAEVLRRYGV
                     RAVIGKGGMGARTLAGLKETGAVYLNGIGGAAQYYARCIEKVLGVHWTQFGIPESMWH
                     LRVRNFPAIVTMDSHGNSLHADVEARTGEALEAVQKA"
     gene            complement(779..2008)
                     /locus_tag="VFQ79_11150"
     CDS             complement(779..2008)
                     /locus_tag="VFQ79_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0140264.1"
                     /translation="MRRRDFLQAAGGLLAAGGTLLPAQAPGEIGAVPEWEAPLLALDK
                     WVKAPVRIQSIELLRLKNNYFVRARSTDGLEGIVQTKQVDDYIPILLRRVIPHFLGKD
                     ARELERLVDSVYTANYKMAGQPFWAPVAYVEQCLFDLLGKAANKPVGVLLGGVVRKEI
                     PVYLSGSTRDTSAEQEVEIYERGVSQTGAKAVKFKIGGRMSRNADAYPGRTEKMMKLA
                     RQKLGAEVVIYADANGSYDAKKAIDIGRMLEDLDIGFFEEPCPWEERSETRKVADALK
                     MFVAAGEQDSSAWLFHWMIENRVMNIVQPDLNYNGGFLRTARVARMAQKAGMKIVPHN
                     TQTGPASVNILQFASAIPNIGPYMEFPWRKPHTAESWYSPQFAIRNGVLQVPQGPGLG
                     VEFDPQYLARATKVQAE"
     gene            complement(2010..2927)
                     /locus_tag="VFQ79_11155"
     CDS             complement(2010..2927)
                     /locus_tag="VFQ79_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0140265.1"
                     /translation="MPTRRQFLATTFAVPAAWTRTSVGRGRISAITDEIAKSPQEAIA
                     FARQYGLSHVELRSVPGGGGEYARLPEDRLREEARKLSENGLKVSFLNTGMLKFGMPG
                     TEPVRRREESLEQRAKRIAAERKRFDEHISDLEKAIRAAQIFNVDKVRVFTFSRVADP
                     GSLYPKIAMILDGMARIARKQGIHLLIENEGSCNVGTCSELARILELAPASNVGINWD
                     PLNGVGLKEAPFPDGYALLPKQRIGNVQIKGRSILDGPQKLDWTAIFEALERDGYKGM
                     FGLETHIFGPTLIESSHASIREILRIAGS"
     gene            3107..>3561
                     /locus_tag="VFQ79_11160"
     CDS             3107..>3561
                     /locus_tag="VFQ79_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140266.1"
                     /translation="MTRVDHWCWSLTLVFASGMCAQHPPQPAAEPEDAVECPATDAAR
                     GENAVPEKPERYGLPVLGPSRPTHPSAGPLTLSEKFRYYLAAYEPHDFARSAFWAAIA
                     QWRDSTPEWGQGMEGYGRRFGSRLATHTIRRSLQFGIGAIRHEDPRYIGS"
BASE COUNT          688 a         1086 c         1037 g          750 t
ORIGIN      
        1 cgctcatcgg ctgcaagatc gccgcggcga atcgtctgcc cgccagtttc tttgtatccg
       61 tcgcctatga ctgctgggcg ttccggcggc tcggcgtccg gctggacgcg cgcgatggag
      121 cgattacgga atggctctac cgcgatcccg agcgtcccgt cgcgaagatg gctgcctcca
      181 ccggcttctc cctcaccgga cgcgaagtag tgctcacacc gccgctgacg gaagaagccg
      241 tccgcgcgct gaaggtcggt gacgtggtcc tcgtgacagg cgaaatattc accggacgcg
      301 acaacgttca tcaccacctg atgtcgcatg aacccccggt ggaccttcgc ggagccatca
      361 tttatcactg cggtcccgta atgctgaagc agggtgaaga gtggacggtg aaagccgcgg
      421 gtccgaccac cagcagccgc gaggagccct accaggcgga ggtactgcgg cgttacggag
      481 tacgcgccgt gatcggaaaa ggcggtatgg gagcgcgaac gctcgccgga ctaaaagaaa
      541 ccggagcggt ctatctgaat gggattggcg gcgccgctca atactacgcg cgctgcatcg
      601 agaaagtgtt gggagtgcat tggacgcaat tcggcattcc ggaatccatg tggcacttgc
      661 gcgtccgaaa ctttcccgcc atcgtcacga tggattcgca cggcaacagc cttcacgccg
      721 atgtggaagc gcgaacgggc gaagccctgg aggcggtgca aaaggcgtaa cccggctgct
      781 attccgcttg aactttcgta gcgcgcgcaa ggtactgcgg atcgaactcc acgcccagcc
      841 ccggcccctg cggtacctgc aaaactccat tccggattgc aaactgcggc gaataccagg
      901 attcggctgt atgcggcttg cgccatggga actccatgta gggtccgatg tttgggatgg
      961 ccgacgcaaa ctggaggatg ttcacacttg ccggacccgt ctgcgtgttg tgcggcacga
     1021 tcttcattcc ggctttctgt gccatccggg ctacgcgggc cgtgcgaagg aaaccgccgt
     1081 tgtagttcag gtccggctgc acaatgttca tcacgcggtt ctcgatcatc cagtggaaaa
     1141 gccaggcgct cgaatcctgc tcgcccgcgg ctacgaacat tttcaaggca tcggcaactt
     1201 ttctggtttc gctgcgctct tcccacgggc acggctcctc aaagaaaccg atgtccaggt
     1261 cttccagcat cctgccgata tcgatcgcct tcttcgcgtc gtaggagccg ttcgcatcgg
     1321 catagataac gacctcggca cccagtttct ggcgcgccag tttcatcatc ttttccgtgc
     1381 ggcccggata agcgtccgca ttccggctca tgcggccgcc gatcttgaat ttgacggctt
     1441 tggctcccgt ctggcttacg ccgcgctcgt agatctccac ttcctgctcg gcggatgtgt
     1501 ctcgggtgga gccggaaaga tacaccggaa tctccttgcg gacgactccg cccagcagta
     1561 cgccaacggg cttgttcgcg gcctttccaa gcaggtcgaa aaggcactgc tccacatacg
     1621 cgaccggcgc ccagaacggt tgtccggcca tcttgtagtt ggcggtgtac accgaatcca
     1681 ccagccgctc caactcgcgt gcatctttgc ctagaaaatg cgggatcacg cggcggagaa
     1741 gaatcgggat gtaatcgtca acctgcttgg tttggacgat gccctccagg ccatcggtag
     1801 aacgcgcgcg cacgaagtag ttgttcttca ggcgcagcag ctcgatgctc tgaatgcgaa
     1861 ccggagcctt gacccatttg tccagcgcca gcagcggcgc ctcccattcc gggacggctc
     1921 cgatctctcc gggtgcttgc gcggggagaa gtgtaccgcc agcggccaac aggccgcccg
     1981 ctgcctggag aaagtcgcga cgcctcatgt cacgacccgg cgatccgcag gatttcccgt
     2041 atcgaggcat gcgacgactc gatcagcgtc ggaccgaaaa tgtgtgtttc caggccgaac
     2101 atccctttat acccgtcgcg ctccagcgcc tcgaaaatgg cagtccagtc cagtttctgc
     2161 gggccatcga gtatgctgcg gcccttgatc tgtacgtttc cgattcgctg tttcggaagt
     2221 agcgcatatc catccgggaa tggcgcttcc ttgaggccga ctccgttcaa aggatcccag
     2281 ttgatgccga cattcgaggc cggcgccagt tcgagaatgc gagccagctc agagcacgtt
     2341 cccacgttgc acgatccctc gttctcgatg agcaggtgaa tgccctgctt acgggcgatt
     2401 cgcgccatgc cgtcaaggat cattgcgatc ttcgggtata gcgagcctgg atccgccact
     2461 cgcgaaaatg tgaacacacg gaccttgtcc acattaaaaa tttgtgctgc ccggatcgcc
     2521 ttttcgaggt cggagatgtg ctcatcgaac cgtttccgtt cagcggctat ccgcttggca
     2581 cgctgctcca ggctctcctc gcgccggcgc accggttctg tgcccggcat gccgaacttc
     2641 agcattcctg tgttcagaaa cgataccttc agcccgttct ccgacaattt tcgcgcttct
     2701 tcacggagcc ggtcctccgg aaggcgtgcg tattcgccgc caccgccggg aacgctccgc
     2761 aattcgacat ggctcagccc gtactgtctg gcaaaagcga tggcttcttg cgggctttta
     2821 gctatttcat ccgtaatggc gctgattctg ccgcggccaa cggacgttct ggtccaggca
     2881 gccggtacgg cgaaagttgt agcaaggaac tggcggcggg ttggcaacag gttgacctcg
     2941 tgacgagttt ctcacaaaat aacattccac aactcatgtc tgcgaagtta ttacaacagc
     3001 ttccgggcct gaaatggtaa gtctctgtat ataattgaaa tcatttagct ttaaagaaga
     3061 tatagaatgg acggaaacgt gcattccggg cttgtggagg aatgatttga cccgtgtgga
     3121 ccactggtgc tggtcgttga cgctggtttt cgcgtcaggg atgtgcgcac aacacccccc
     3181 gcagcctgca gcggagccgg aagatgcagt cgagtgtcct gcgacggacg cggcccgcgg
     3241 ggagaatgcg gttcccgaaa agccggaacg ctatggattg ccggtgctcg gtccttcccg
     3301 tcccacgcac cctagcgcag gcccgctaac gctcagtgaa aagttccgat attatctggc
     3361 ggcatacgaa ccgcatgatt tcgcccgttc cgccttttgg gcagctattg cgcaatggag
     3421 ggactccacg ccggaatggg ggcagggcat ggaaggatac ggacgccgtt ttgggtcgcg
     3481 cctggccacc cacaccattc gccggagtct ccaattcggc attggagcaa tccggcatga
     3541 ggacccccgc tatatcggtt c
//
LOCUS       DASTMK010000260         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_524945, whole genome shotgun sequence.
ACCESSION   DASTMK010000260 DASTMK010000000
VERSION     DASTMK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524945"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>2923
                     /locus_tag="VFQ79_11165"
     CDS             66..>2923
                     /locus_tag="VFQ79_11165"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140267.1"
                     /translation="MQGIVTDPTTAAVPDAKVTLQNDNTGVASTRATNTSGQYLFDYV
                     EPGTYTVSVEAAGFARTIQRNVTVQVRGDVTVNLTLTVGAVAESITVEAAPVAVQFNT
                     TSRELTIDRKLISELPIIARNPFTLALLDPAVVNRYSQDRYPYFMWSSSQIDVGGSTS
                     SRNDLLLDGVPIQLGQKGSYSPPMDSVQEFTVQQNAVDAEFGHSAGGTMSLSMKSGTN
                     EFHGTAYYFGRNPKLNAVANSVTRRANVSRNHIAGGTLGGPVLRNKLFNFVTYEWWKT
                     PQSLSTIQSLPTELERSGDFSRSLTAAGAMRAIYDPWTTQFDAAANRATRMPFAGNVI
                     PLSRIDPTSRRVLQDLWMPNSPPDDLTGLNNFKLGFPRYHRYWNFSDRADWNISDRWR
                     VFGRYSRFLNDYQPVNYGNSPAVPVNGGIMASRSLAFDSVYSLTERTILNVRVGYTSL
                     EDDLDTPSTEISEAELESFWPGNPWYKSYISNLDNLYYPQMNVQGAGGLNFGRGAPWI
                     QHPSSYSYHGKVSHARGIHQLKAGFEGRRAQGIQHTPYPFQFEFSPNQTSDTFIAPDT
                     RLRGHPWASFLLGALEGTSRAQYISALDLRSNFYGGYVQDDIRLTRNITVNLGLRYEL
                     ETGLRDTENRVSRYLDLTDPIPEMQAAPPPIPDSVRSLNNVPYAYNGAWVFTDDNHPN
                     AYETPKNRFMPRAGIAIRVNDQTAVRVGFARYVIPAEVVNGTLDTLPTYGFNAATNVE
                     PVLQGVPGAVLSDPFPASNPLIQPLGRALGRYQNLGDVATWLGPELRTGVNDRFNFSL
                     QRQLPAQIVADATFFMNYGRDLGYSKQLNMMDPALSYLYKAELSRSIANPFYQYLTPD
                     KFPGQARNRQNVTVGSLLVPYPQYGTLTRNQVSGKRDRYRALQLRLQRSSASGYTFLW
                     AYNYNRQRTDEFFNSDDQYAERFTLIPSNNPRHRMTLAG"
BASE COUNT          595 a          982 c          806 g          540 t
ORIGIN      
        1 agcgtgtaat tctctcgttc ctgctagttt ccgcagtcgc attttctcaa gattaccgcg
       61 ccaaagtgca aggcatcgtc acagacccga ccaccgccgc cgtgccggac gccaaggtga
      121 cgctgcagaa cgacaataca ggagtggcaa gcaccagggc cacgaacact tccggccaat
      181 acctgttcga ctatgtggag cccggaacct acaccgtcag cgtggaggcc gccgggttcg
      241 ccagaaccat ccagcgtaac gtcaccgtgc aggtgcgcgg cgacgttacg gtgaacctga
      301 cgctcacggt tggcgccgtc gccgaatcca tcaccgtgga ggccgcgccc gtagcagtcc
      361 agttcaacac caccagccgg gaattgacaa ttgatcgcaa gctcatttcc gaactgccga
      421 ttatcgcccg caacccgttc acgctggccc tgctggatcc ggcggtcgtc aaccgctata
      481 gccaggaccg ctatccctat ttcatgtggt cttcgagcca gatcgacgtc ggcggcagca
      541 cgtccagccg taacgacctg ctgcttgacg gcgttccgat tcaactcggg caaaagggtt
      601 cctattcgcc gccgatggac tccgtgcagg agttcacggt gcagcagaat gctgtggacg
      661 ccgaattcgg acacagtgcc ggcggcacca tgagcctctc catgaaatcc ggcacgaacg
      721 agtttcacgg caccgcgtac tactttgggc gcaacccgaa actgaacgcg gtggcgaact
      781 cggtcacccg gcgcgccaac gtcagccgga accatattgc cgggggcacg ctgggcggac
      841 ccgtcctgcg caacaagctg ttcaacttcg tcacgtacga gtggtggaag acgccccagt
      901 cgctctccac catccaatcg ctgcccacgg aactggagcg ctccggcgat ttctcccgct
      961 ccctcacggc cgcgggggcc atgcgggcga tctatgatcc gtggacaacg cagttcgatg
     1021 ccgccgcgaa ccgcgccacg cgcatgccgt tcgccggcaa tgtgatcccc ctaagccgga
     1081 ttgaccctac gtcgcgccgc gttctccagg atctctggat gccgaacagt ccgccggacg
     1141 atctcacagg cctgaacaac ttcaagctgg gcttcccccg ctatcaccgc tattggaatt
     1201 tttcagaccg cgccgactgg aacatcagcg atcgctggag agttttcggc cgctacagcc
     1261 gttttctcaa cgattaccag cctgtcaatt acggcaattc gccggcggtg cccgtcaatg
     1321 gcggcatcat ggcatcgcgc agcctggcgt tcgattcagt gtactcactc accgagcgaa
     1381 ccattctaaa tgttcgcgtc ggttacacct ctctggaaga cgacctggac acgccctcga
     1441 ccgaaatctc ggaggcggaa ctcgaatcct tctggccggg caacccctgg tacaagtcgt
     1501 atatctcgaa cctcgacaac ctgtattatc cgcaaatgaa cgtacaagga gccggcggct
     1561 tgaactttgg ccgcggtgcg ccgtggatcc agcaccccag cagttacagc tatcacggca
     1621 aggtgtcgca cgcccgcggc atccaccagt tgaaggcggg ctttgaagga aggcgcgcgc
     1681 aagggatcca gcacaccccg tacccatttc agttcgagtt cagccccaac cagacctccg
     1741 acacgttcat cgccccggac acgcgcctcc gcgggcatcc ctgggcgtcg ttcctgctgg
     1801 gagcgctgga aggcacttcc cgggcgcagt acatctccgc gctcgatctg cgttcgaatt
     1861 tctacggcgg ctacgtgcag gacgacatcc ggctcacccg caacattacg gtgaatctcg
     1921 ggttgcgcta tgaactggag accggccttc gcgacacaga gaaccgggtg tcgcgctatc
     1981 tcgacctcac cgatcctatt cccgagatgc aggcggcgcc ccctcctatc ccggacagcg
     2041 tgcgcagcct gaacaatgtc ccgtacgcct acaacggcgc gtgggtcttc acggacgaca
     2101 accatccgaa cgcgtacgag acgccgaaga accggttcat gcctcgcgcc ggaatcgcca
     2161 tccgggtgaa cgatcagacc gctgtgcgcg tgggattcgc gcggtatgtc attccggccg
     2221 aagtggtgaa tggcacgctc gatacgctgc caacgtacgg cttcaacgcg gcgaccaacg
     2281 tggaacccgt gctgcaaggc gtgcccggcg ccgttctgag cgacccgttc ccggcatcga
     2341 atccgctgat ccagccgcta ggccgcgcac tgggccgtta tcagaacctg ggcgacgtcg
     2401 cgacatggct cggccccgaa cttcgcacgg gcgtaaacga ccgcttcaat ttcagcctgc
     2461 agcggcagtt gccggcgcaa atcgtcgccg acgccacgtt ctttatgaac tatggccggg
     2521 acctgggata cagcaagcag ctcaatatga tggatcccgc gctcagctac ctgtataaag
     2581 ccgagttatc gcggtctatc gcgaatccct tttaccagta cctcacgccg gataaattcc
     2641 ccggccaggc gcgcaaccgc cagaacgtta cggtgggcag tttgctggtt ccttatccgc
     2701 agtacggcac tctgacccgc aaccaggtct ccggcaaacg agaccggtac cgcgcgctgc
     2761 agttgcggtt gcagcgttct tccgccagcg gatatacgtt cctgtgggcg tataactaca
     2821 accgccaaag gaccgacgag ttcttcaatt ccgacgacca atacgcggag cgtttcaccc
     2881 tcatcccaag caacaaccca cgccaccgga tgacattagc cgg
//
LOCUS       DASTMK010000261        21867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_620653, whole genome shotgun sequence.
ACCESSION   DASTMK010000261 DASTMK010000000
VERSION     DASTMK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21867
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620653"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..700)
                     /locus_tag="VFQ79_11170"
     CDS             complement(272..700)
                     /locus_tag="VFQ79_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140268.1"
                     /translation="MTRRQVAAMLAIPLGFGRLTAQTPPEIAELFGDLAAALSDGRAA
                     DFMRYFDTSMPGYAELRTLVSALLEQAEVSSSIEFINVEQDQADYSVEVDWLLHLRAR
                     SPGEPVDRRRGKLKCRATRKGRAWKVTSLSPVDFFRPPRY"
     gene            complement(703..1881)
                     /gene="argJ"
                     /locus_tag="VFQ79_11175"
     CDS             complement(703..1881)
                     /gene="argJ"
                     /locus_tag="VFQ79_11175"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU0140269.1"
                     /translation="MNLPAGFSYSALYAGIRKVKQDDLALIFSTAPAAAAAVFTRNRV
                     QAAPVRLARKHLESSGGEAQAILVNAGNANCATRTGDRVVMACCQAAAKGLKVKTNRI
                     LPASTGVIGVELDPALITGAMPRLIEGLSADRFDDVSRAILTTDTSAKTAFAEVAVKD
                     GIVRIAGMTKGAGMIQPNMATTLGFVLTDAAIPTLVLRPMLKKVVDLSFNRLSVDGDT
                     STNDVVILLANGASGCKLKAADRQLFSEALQHLMQDLARQIAKDGEGARKRITIEISG
                     TADEASASVIARAIANSPLVKTAIAGSDPNWGRILSAAGNSGVAFDPRKADIWLQDVK
                     VCTSGLAADFSEPELKEKLNGDECVIRFNIRGGGPGRSRFWTCDLTEKYIEINASYRT
                     "
     gene            complement(1931..2893)
                     /gene="fmt"
                     /locus_tag="VFQ79_11180"
     CDS             complement(1931..2893)
                     /gene="fmt"
                     /locus_tag="VFQ79_11180"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU0140270.1"
                     /translation="MELIFLGTPQFAVPTLQAVVEGGHHVLAVFTQPDRPSGRGGKIV
                     FSPVKQKAIELGIPVKQPQRIRGAEAFDFLAGAGADAMVVVGYGQIIPQSVIDLPRFG
                     IVNVHASLLPKYRGAAPVQRAIVNGETQTGVTTMKIDAGLDTGDMLLRAATEIGPEED
                     AIELSGRLSRLGAELLVETLRQMEAGTIIPEPQDNSGATYAPVLKKEDGLINWNESAM
                     SIHNRVRGLVPWPGAYTYFRGQLLHVWKAKFAAEQTEGIPGTLHPVRRSLRVACGGGT
                     SLELLDVQLEGRKRTTAESFLNGQRILEGEPLAGARGSVTEPRP"
     gene            complement(2884..3399)
                     /gene="def"
                     /locus_tag="VFQ79_11185"
     CDS             complement(2884..3399)
                     /gene="def"
                     /locus_tag="VFQ79_11185"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0140271.1"
                     /translation="MIYPIVKYGDPVLEKRAARVEEFGTPDLRKFIDDMFESMYAARG
                     VGLAAPQIGFSKRIAVIDCSAGEDPSQKVVLINPEIIAKEGSQTGEEGCLSVPGFREQ
                     VTRANKVTVRAQNVEGESFEMTGEDLLARAFLHETDHLEGRLYISHISALKRDLIRRK
                     VRKLVKAGEWS"
     gene            complement(3399..4571)
                     /gene="aroC"
                     /locus_tag="VFQ79_11190"
     CDS             complement(3399..4571)
                     /gene="aroC"
                     /locus_tag="VFQ79_11190"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU0140272.1"
                     /translation="MLRFETAGESHGECLVATLTGMPAGVPISLDAINRELWRRQQGY
                     GRGGRMKIETDRVEVVAGVRHSQTIGSPIALIIRNSDWQNWTEALPVEDGEGAEDKKK
                     PVRRPRPGHADLAGCIKYNFHDARYILERASARETTARVAVGAIAKAFLAEFHISVLS
                     HVVAVGPVTLEREASWEEIVELSKRDKVLLGCVDAATEQQMKEVVDEVYRTGDTAGGV
                     FEVVVHGVPPGLGSHITWDSRLDGKLAQAIVSIQAVKGVEVGRAAQGAASFGSSVQDT
                     IHYDRTTRHFLRGANRAGGIEGGMTNGEDIVVRGLLKPISTLRRPLESVDLLTREPSR
                     AAYERSDVCVVPAAGVIGEAMVAIVLAQAFLEKFGGDSLGETRRNFEGYIEQVKNF"
     gene            4639..5052
                     /locus_tag="VFQ79_11195"
     CDS             4639..5052
                     /locus_tag="VFQ79_11195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0140273.1"
                     /translation="MVEHEMTLRVRYAETDQMGVAYYANYLVWMELGRVEYCRAAGLR
                     YRDMEENDQVLLAVVEASCRYVSPARYDDEVIVKTWTEHSTRRTVTFGYEMRIAGSNR
                     KIATGETRHVFCGPDMKPRKLPEKYGELFGVGING"
     gene            complement(5062..5859)
                     /locus_tag="VFQ79_11200"
     CDS             complement(5062..5859)
                     /locus_tag="VFQ79_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0140274.1"
                     /translation="MLRSLSMLAMLAVLPLCGATKVVVTVVEQKTGAPVTGLKGLDFT
                     VSDDKVERKVESVEYTKVMADIMLLVDTSLVGPVVQPVAAKLIGQLQEKEQMALVSFH
                     SSADLIQDFTSSRDALLRALGNVKYGNSPRMLDALYAAIDGGFQSSNFRRVILLLTTG
                     LEGPSRMSERQVVRLARQNGVSIYPVFVGGYGRSLFESLARQTAGAPFSLREMEREHV
                     DDPSARIFEVIRNHYSLSVAGNLGLGEKFKITVKYPRKTFVSALPLE"
     gene            complement(5868..6938)
                     /locus_tag="VFQ79_11205"
     CDS             complement(5868..6938)
                     /locus_tag="VFQ79_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140275.1"
                     /translation="MESVGTLFPYDEVLISAEVDGRVDRVLVDLGDSVAQGQLLVHIS
                     EEEQQYLLSQNEAQLRQSLERLGLSSEKDRVKDIRETPEVRGAQADLFDAEQRHKRVR
                     ELVDQGIGAHADLDQAVARFKAAQAAYDASINQTRNLIQEVERFKAAVELQRKKLRDT
                     SVRAPFASYVKERQVTVGQYVRANTPLITLVKVDPIRLRVEIPERMAPWIKNGQVAAV
                     SVEAFGDRIFNGTVWRISPTVDQSKRTFVVEALIQNPRGELKPGFYAKARVPTDKIDE
                     VKLVPVRAVNYVLGSNKAYVVKDGSIDAREIKLGDRFDESVEVLEGLNVGEQVAITQL
                     SRLDSGVKVRVTDTADNPRTSD"
     gene            complement(7190..8305)
                     /locus_tag="VFQ79_11210"
     CDS             complement(7190..8305)
                     /locus_tag="VFQ79_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEU0140276.1"
                     /translation="MQVPGPLVRGLSRRGLLLSVAFPAFAGKLEKRELLPSEKRWYPD
                     PSTEFPVVRLTNPEHSSHLTSSSLKSISRRSDFLLYSCDRTGSPQVFRMDLKSGESSQ
                     LTEAEFLDPLSLVLMPDEKSFCYFDGPALMQVELSKLRERRIYEVPGRYHRGDAALSI
                     AANGTHAMVVENTDGSSRLIVVPLRRGSPSLAVQSEGVISLPLARPGRQEILYRKDDG
                     STWLTDYTGRNNRKLPLAAGKNGRQAWSPDGKTLLYLNTPEDRSRLNVLREFTPESDT
                     DGLIAETSQFVAFARNTDASVFVGASANRASPYILLLIRAVRRELTICEHKSTMPERT
                     APIFSPNSRQVFFESDRHGKPALYTVNVEKLVESTES"
     gene            complement(8324..9331)
                     /locus_tag="VFQ79_11215"
     CDS             complement(8324..9331)
                     /locus_tag="VFQ79_11215"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEU0140277.1"
                     /translation="MILLTSAYKRIHSADTMAQTARAFLNSLSAEQVAKTRFAFQDDE
                     RFFWHFIPSDDIPKRYGRPRKGVPFLEMTPMQRHLAHALLSAGLSQSGYVKASTIMSL
                     EEVLRQIERSPSGRRDPEKYFFSIFGDPSDKEPWGYRVEGHHLSLHYTVVNGKISGSP
                     LFLGANPARVPSGPMEGLRILAREEDLGRDVISALDSTQRQTAIVSKTAYPDILTAAD
                     REAALKGQPTGLSAAKMSGKQRELLQVLLAEYAHNLPEDAAQARIEQIKKTGNDLHFA
                     WAGSIERGGPHYYRIQAPSFLIEYDNTQNNANHIHSVWRDFTGDWGADLLKEHYRSGH
                     K"
     gene            9534..10829
                     /locus_tag="VFQ79_11220"
     CDS             9534..10829
                     /locus_tag="VFQ79_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU0140278.1"
                     /translation="MYSGQRPEAIDSRWVFRIYAAMACLTGFLLTGWGLIWFGADIPG
                     QPWGKAALIRVFGSIVMAAGFCAAGFAAIDQPLARRRGLFWFATAHAVVWLVVLTQQI
                     AIWDSGLAEKVTQLLLCPVLIFYYLWATPTGESAYRILIGLFEGNRQSSAEQLRSQYE
                     RQIREVAKQEERNRLARDLHDSIKQQIFVIQTAAATAQARFDGDQTGAKQALDQIRGS
                     AREAMTEMQVMLEQLRAAPLENTGLVESLKKQCEALTFRTGARAEFKLGNLPAAEMLP
                     PGAHEAILRVAQEALANIARHARAGSVVVSLGSVHGHIELKVQDDGAGFDPNQSDGGQ
                     GIRNMKARAEEFGGTFELVSGPGSGTSVALAIPFTAAETPRVYRRKAAAWAFMLVISI
                     AILSWTRPIPIAVIAIAIVGFARYLVAYRRAQMRSEAAQ"
     gene            10826..11467
                     /locus_tag="VFQ79_11225"
     CDS             10826..11467
                     /locus_tag="VFQ79_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0140279.1"
                     /translation="MTPVSIALVDDHRVVAHSLKAYLESFPDLRVVGIATSGEELLRR
                     IDDWKPQVVLQDLLMPGGIDGIETIRRISERAPGVKVIALTASIDEARMMGSLRAGAA
                     GYVRKDAEPEILLAAVRAVARGGTYIDPSVSRQILRAVVPHEDLTPREIGVLRQLALG
                     RSNKEIADALSIGDETVKTHVGNVFAKLQVENRAQAIVQALKRGLVSLEELEQ"
     gene            complement(11478..12359)
                     /locus_tag="VFQ79_11230"
     CDS             complement(11478..12359)
                     /locus_tag="VFQ79_11230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0140280.1"
                     /translation="MNQRYFILVLAHSVHGRLRRVHIPQRVVYGVLALALLGCFSLFG
                     IVSSYARMAWKVADYNSLRTEFNTLRARYQNLQNSANQTKEQLATLQVFATEVSIAYG
                     IKQKLEGTTDISSEGRLVPTFSESLAEYNFLKGASFSKLRRRYPSYWQTNVRPDMWPV
                     NGRLMSHFGRRSDPFSGEWLFHSGVDISAATGTPVQATADGVVVHAEYSGAYGKLVVV
                     DHGDLQTYYAHLSRIDVVEGQEIRRGEIVGGAGATGRATSSHVHYEVRQNRTPVNPYP
                     FLKTMVAQEFRKEDLPF"
     gene            12608..13510
                     /locus_tag="VFQ79_11235"
     CDS             12608..13510
                     /locus_tag="VFQ79_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0140281.1"
                     /translation="MTRRELLAAGMGAMVRPAAAQDRTKFQIGCMTLPYAPFPLERAL
                     QGLAKAGCRYIGWGTTHMESSGQRVPVMGPDAPPSEARQLARKCRDLGLEPVMMFNIT
                     YVEAPDSIKVHTSRIEQAAAAGIPFIITFGKVSPGGSGGYDVWIKNLKQLGSIARQNG
                     VIITIKQHGGNTATGKDCSKIIADVADDGVKMCYDAGNVLDYQSVDPIPDIQACWRDI
                     RSFAIKDHRNTPKDEDCGPGFGEIDHYKLLMPVMRTGLTIPLICENIFEPLVRRPSSP
                     DGVDALARRAREYVETVVRGLESV"
     gene            complement(13518..14624)
                     /locus_tag="VFQ79_11240"
     CDS             complement(13518..14624)
                     /locus_tag="VFQ79_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140282.1"
                     /translation="MNRRSFLMSSIASSTALFGQSSGDAIGTGMIGTGNRGAYLLKTV
                     LGLPAAKVVAVCDIKPDRLDKAATAAAEHKPKTYTDYRRLLEDKDVEAVYIATPCDLH
                     VEMAIAALQAGKHVYCEKPVGITPESIGRLLEVARNSNKVFVPGQQMRSMERIRQTIE
                     KIQKEGVLGKVIMVKAQRHAGDDLDHQGSSADWFFDAKRSGDVIVEMAVHNLDLCNWV
                     IGSRPARAGGFGGALLWKNDPPGRTSMDGYTLSYDYENDVKMSFTQVFFHPNGLPSNG
                     QFVHVYGTEGAVDLNRATFYKRARKAEPVVLVPEIKENNEAHMAAFFEAIRTGKQPPA
                     DLKIGAIAALTAIMGREAIYQKRVFTWDEMGVNV"
     gene            complement(14648..15796)
                     /locus_tag="VFQ79_11245"
     CDS             complement(14648..15796)
                     /locus_tag="VFQ79_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140283.1"
                     /translation="MRTLFAVLVLVLPLAAQSPQTSTFEGQPALAISSGKLDVVILTT
                     GATIASVAVRDDPEQLNPLWNPIRMARELGRPAGGGAGLGHFLCVDGFGSVSAEERAA
                     GLPGHGEAHRQGFEILVSEKSGGVTTLRMSAKLPILEEAVTRTYRMVDGENILYVDTE
                     IESLLAFDRPAVWAEHATIGSPFLEPGKTVVDISGSRSQTRPYEQSQGGRGLAKRLAS
                     GVDFTWPMAPLAAGGTVNLRAAPAEPDSGDHTTTLMTGAKYAWVVALHPGKQLLTGWL
                     FNPEEFPWLQTWENYPPTGKMARGLEFSTQPYDVPRREAIQQGSMFGAPVYRWLPAKS
                     KITSRFILFYTRMPRDFEKVDDVAVEDGRIVIKSGTKAVTLARSLPLW"
     gene            15890..16621
                     /locus_tag="VFQ79_11250"
     CDS             15890..16621
                     /locus_tag="VFQ79_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU0140284.1"
                     /translation="MKNLEVATSREISASPSRQFKYLDFFIYVFVVVLLVSNIVGPKI
                     TAIGPFRISGAQLLFPITYIFGDIFTEVYGYAASRRAIWAGFFASALLSVMGLFAVWL
                     PPAPGWEGQEAFAQVLGFVPRMVAASLIAYWCGEFTNAFVMSRMKVLTGGRYLWMRTI
                     GSTAIGQLVDTALVMTLAFGGTVRVSLLLNLILSGYLGKVLYEAAATPVTYAVVNFLK
                     RKEGVDVYDYATNYNPFEGNIIRET"
     gene            complement(16582..18057)
                     /locus_tag="VFQ79_11255"
     CDS             complement(16582..18057)
                     /locus_tag="VFQ79_11255"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEU0140285.1"
                     /translation="MAWCRLEPLGKAIYIDEGLLLKEVLFSYGVEFPCGGRGKCKACR
                     VRVTGGVLPPVQDELRILSSEEIEQGWRLACRSVVRDDCVLEIAQWESPILTDNRPFH
                     FTPREGFGVAIDLGTTTVVAQLIDLATAQVHAVNTALNGQAVHGSDIMSRVQHALTPS
                     GAKELRDIIRCGLGAMIAGFDVPAIADVVIVGNTVMHHLFCGIDVEPLSHYPFEPVRD
                     GLECFQPEELGWPRGGRIRFLPCLGGFVGSDILAGILATGLHESVEPVALMDLGTNGE
                     VVVGTCDRLYCASTAAGPAFEGGRIGAGMRAATGAISEVSIRDGALISTVIGGGPARG
                     VCGSGLVDAVACGLEAGLVQPSGRLKNGPLTLAPGIALQQSDIRELQLAKGAIAAGLD
                     LLAARCGLAPAALKRIYLAGAFGNYVNRASARRIGLIDFPEEQVEPAGNTSLLGAKMA
                     LFRPDDNFEWLRARVQHVMLNADPEFQDAYVSRMIFPSNGL"
     gene            complement(18143..19072)
                     /locus_tag="VFQ79_11260"
     CDS             complement(18143..19072)
                     /locus_tag="VFQ79_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140286.1"
                     /translation="MLKPFLAFAAGVVVAAGFTVYMMREPAAPEPAASVAAPIPVVES
                     PAVETPAPAEPVNLAPPAAKPSPAPVERRRERKPARTSDPSPPAKPVEVAQTQQPAPQ
                     PAPVVNPVEPPAPAPAPAILQPEKIEPPAPPPPRKVTIPSGTLINVRLAETLNSDKDQ
                     AGDTFFATLDQPLVVDELVIAERGARLEGRVVEAQEAGRVKGLSQLSIQLVRLNTRDG
                     QKVAIQTEPFVKSGPASKGSDVAKVGVAAGLGAAIGAIAGGGKGAAIGAAAGGAAGTG
                     GVMATRGKPAQIDVETRIAFKLTDPVTLTERVR"
     gene            complement(19104..19763)
                     /locus_tag="VFQ79_11265"
     CDS             complement(19104..19763)
                     /locus_tag="VFQ79_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717365.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEU0140287.1"
                     /translation="MAARTPLVTAAPLIPLDPDIGKLREAAAGCQACDLYKRGTQTVF
                     GAGTPQAKVMLVGEQPGNDEDLKGLPFVGPAGKLLDKALASAGIDPKQTYVTNVVKHF
                     KWEPRGKRRIHSKPNQAEISACRPWLDAEIQMVQPKVIVCLGATAAQALLGRQFRVTQ
                     NRGQFVESPLAPFMLATVHPSSILRAPDEETRRAEMERFIADLSVAASVLSVGRNAGR
                     V"
     gene            19805..21007
                     /locus_tag="VFQ79_11270"
     CDS             19805..21007
                     /locus_tag="VFQ79_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0140288.1"
                     /translation="MRPFHLLLVLPLMLPAQQAREESFLGSLAPVIRSIQEERGFPMA
                     YENRKGISVDEWRRRGRAEVQRCLSFSPKPVPLDVKVHSVVERDGYEVRKISFAGSGH
                     YRVPAYLLVPKKSGQKLPAVVALHDHGGWFFHGKEKLVKLEDEHAALTAFREQYYGGR
                     TYADELARRGFVVIVPDAFYWGERRLTYEQPPKELASRLARHDPASAEYVRAYNSWAR
                     ERTTELNTWLSFSGTSWMGIVNHDDRRSVDVLASLPEVDPKRIGCLGLSGGGYRSTYL
                     AGMEPRIRAAVITGWMTSLPTTLDISYSVHSSLFDAFGVHQNLDHSDIGSLAAPDCAV
                     FVQNCARDRLFTRAGMESAAEKIRAVYTDLKRPERYRSKFYDVPHQFNVEMQEEAFAW
                     LEQWLSKP"
     gene            21201..>21867
                     /locus_tag="VFQ79_11275"
     CDS             21201..>21867
                     /locus_tag="VFQ79_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0140289.1"
                     /translation="MIAVSRAATLACAAVLTFAGEVPLAQQVASEIEQPSVVFSTTAR
                     LVHVNVVAEDKQGRPMADLRKEDFEILDNGVSQSIAFFTAEHTIAPNRLVAVEGVFTN
                     QLPVATGWRSGYSVILLDWLNTAWGDKVYARNQALQMLTSVGVHDQVALYVLGNGLRV
                     VHEFTADTVALARKIAAEQGQVVDAPDPEVNPLTDASVGSLPFSDIGPGGLSATKREE
                     RFFI"
BASE COUNT         4335 a         6735 c         6287 g         4510 t
ORIGIN      
        1 aaggacatcc cttaaggaga atctaaggta aatccccgat actggttctg taggaaagat
       61 gttttgcgcc tattcaggat attaaggctc ttaatactgt tgttcattcc attactgtgc
      121 gccctatccg ccgcctcgga cccggaagga gacgagccgt catcgatccc ggagccggtg
      181 accatgattc tgctcggcat aggcctggtg gcattcggtg cgcgggctct ccggaagaga
      241 tcaaccaccc ggcggcgctg aacggcgctc gctaataacg gggtggtcga aagaaatcga
      301 cgggggagag ggaggttact ttccatgccc gccctttccg ggtggcacgg cacttcagct
      361 tgccgcgcct tcgatcgacg ggctcgcccg gcgaccgcgc tcgtagatgg agcagccagt
      421 caacttcgac cgagtaatcc gcctgatcct gttccacgtt aataaactca atcgacgacg
      481 acacctcagc ctgctccaat agcgccgata ccagagtgcg cagctccgca tacccgggca
      541 tactcgtatc gaaatatctc atgaaatcgg ccgctctgcc gtcgctgagc gcggctgcca
      601 gatctccgaa cagctcggcg atttcgggag gagtctgggc cgtcagacgc ccgaacccca
      661 gcggtatggc cagcattgcg gcaacctggc gccgtgtcat ggtcatgtcc tgtaagaggc
      721 gttgatttcg atgtacttct ctgtcaggtc gcaagtccag aagcgcgatc tgccgggccc
      781 tccgccgcgt atgttgaacc gaatgacgca ctcatcgcca ttcaacttct ccttcaactc
      841 cggctcggag aagtctgccg ccaggcccga cgtgcatacc ttcacatcct gaagccatat
      901 gtcggccttg cgagggtcga acgcgacgcc ggagttgccc gccgccgaga gaattcgccc
      961 ccaattcggg tccgagcccg cgatggcggt cttgacgaga ggcgagttgg cgatcgccct
     1021 cgcgatgacc gatgccgaag cttcgtccgc cgtgccggag atttcaatcg tgatgcgctt
     1081 gcgcgcgccc tcgccgtctt tcgcgatctg ccgcgccagg tcctgcatga gatgttggag
     1141 ggcttcggaa aacagttgcc ggtcggcggc tttcaacttg cacccggacg caccgttcgc
     1201 cagcagaatc acgacgtcgt tcgtcgacgt atcgccgtca acggacaacc ggttgaagct
     1261 gagatccacc actttcttca gcatgggccg gagcacgagc gtcggaatcg cggcgtccgt
     1321 cagcacaaac ccgagcgtag tggccatgtt gggctggatc atgccggcgc ctttcgtcat
     1381 gcccgcgatg cgcacgatac catctttcac cgccacttcg gcaaaagctg tttttgcgga
     1441 ggtgtcggta gtaagaatgg cgcgggagac atcgtcaaag cggtccgcgg atagtccttc
     1501 gataagccgg ggcatcgcgc cggttattaa ggccggatcg agttccactc caatcactcc
     1561 agtggaagcg gggaggatcc ggttggtctt caccttcagg cctttggcgg cagcctggca
     1621 acatgccatc accacgcgat cgccggtgcg cgtggcgcag ttcgcgtttc cggcattcac
     1681 cagaatcgcc tgcgcttcgc cgcctgacga ttccaggtgc ttgcgcgcga gccggaccgg
     1741 cgccgcctgc acgcggttcc gcgtaaatac cgccgcggcg gctgcgggcg cagtggaaaa
     1801 aatcagcgcc aggtcatcct gttttacttt gcggatgccg gcataaagag cggaatagga
     1861 aaagcccgcg gggaggttca cgagatgttg gctcctaagt gcacggtttc agaaattcgc
     1921 ttccgcttcc tcacggtcgc ggctcagtaa cggagccgcg cgcgccagca agcggttcac
     1981 cctctaaaat gcgctgaccg ttcagaaaac tttcggccgt cgttcgcttc ctgccttcca
     2041 gttgtacgtc caacaattcg agcgacgtgc ctccaccaca cgccacccgg agtgagcggc
     2101 gcaccggatg gagcgttccc ggtatgcctt ccgtttgctc cgccgcgaat tttgctttcc
     2161 acacatggag caactgccca cggaaataag tgtatgctcc cggccagggt acaaggcccc
     2221 gaacacggtt gtgaatggac atcgcgctct cattccagtt gatgagacca tcctcttttt
     2281 tcaacaccgg cgcgtaggtt gcccctgaat tatcctgggg ctcggggatg attgtgcctg
     2341 cttccatctg ccgcaaggtc tctaccagga gctccgcacc cagccgggac agcctcccgg
     2401 aaagctcgat cgcgtcttct tccggcccga tctcggtcgc cgcgcgcagc agcatgtccc
     2461 cggtgtcgag accggcgtcg atcttcatgg tggtaacgcc cgtctgtgtc tcgccgttca
     2521 cgatggcccg ctgaaccggt gccgccccac ggtattttgg aagcagcgat gcgtgaacgt
     2581 taacgatccc gaaacgcggc aggtcaatca cggattgtgg aataatctgc ccatatccga
     2641 caaccaccat cgcgtccgca cctgccccgg ccagaaaatc gaatgcttcg gccccacgga
     2701 tgcgctgcgg ctgcttcacg ggaatgccaa gctcgatagc tttttgtttg accggtgaaa
     2761 atacgatctt cccgccgcgc ccgctcgggc ggtccggctg cgtgaaaacg gcgagaacat
     2821 ggtggccgcc ctccactact gcctggagcg tgggcacggc gaattgcgga gttccaagaa
     2881 aaattaactc cactctcctg ccttgacgag cttgcgaact ttcctgcgaa tcaaatcccg
     2941 cttcaatgcg ctgatatggc tgatgtacag ccgcccttcc aggtgatccg tctcgtgcag
     3001 aaaggcacgc gccagaaggt cctcgccggt catttcaaag ctctcgcctt cgacgttctg
     3061 cgcgcgaacg gtcactttgt tggcgcgagt cacctgctcg cgaaatccgg ggacgctcag
     3121 gcagccttcc tcgccggtct gggatccttc tttcgcaata atttcggggt tgatcagaac
     3181 tactttctga gagggatcct cgccggctga gcaatcgatt accgcaatgc gtttggagaa
     3241 gccaatctgc ggagcggcca gcccgacgcc gcgcgccgcg tacatggatt cgaacatatc
     3301 gtctataaat ttccgcaagt cgggagtgcc gaattcctca accctggcgg ctcgtttctc
     3361 cagtacgggg tcgccgtatt tcacaatcgg gtagatcatc agaaattttt cacctgttct
     3421 atgtatcctt cgaaattccg gcgggtctcg cccagtgagt ctccgccgaa cttctccagg
     3481 aacgcttggg ctagaacgat agcgaccatt gcttcaccaa tgacgcccgc cgccggaacc
     3541 acacacacgt cgctccgttc atacgccgcg cgcgaaggtt cccgcgtcag caggtcgacg
     3601 gattcgaggg gacgccggag cgtcgagatc ggcttcagca ggccgcgaac gacgatgtct
     3661 tctccgttcg tcatgccgcc ttcaatgcca cccgcccggt tcgccccgcg aaggaaatga
     3721 cgtgtagtcc ggtcgtaatg aattgtatcc tgcacgctcg aaccgaagga cgccgctccc
     3781 tgcgcggcac gtcctacctc gacaccctta accgcttgaa tcgaaacgat tgcctgcgct
     3841 aatttcccat ccagacggga gtcccaggtg atgtgtgacc cgagtcccgg cgggactcca
     3901 tgaaccacta cttcgaagac gccccccgcc gtatcgccgg tacggtagac ctcgtccacc
     3961 acctccttca tctgttgctc ggttgccgca tccacgcagc cgagaagaac cttgtcacgc
     4021 ttacttagct caacaatctc ttcccatgac gcttcccgct ccagagtaac cggaccgacc
     4081 gcaacgacgt ggctcagcac gctgatatgg aactcagcca gaaaagcctt ggcaatagcc
     4141 ccgaccgcta cgcgggccgt ggtctcccgg gcactcgccc gttccaggat gtagcgtgcg
     4201 tcgtgaaagt tgtacttgat gcatccggca agatccgcat gcccgggacg cggccggcgc
     4261 accggctttt tcttgtcttc ggcaccctcg ccgtcctcca ccggaagcgc ctcggtccag
     4321 ttctgccagt cggaattcct gatgatgaga gcgatgggag atccgatagt ctgcgagtgc
     4381 cggacacctg ccacaacttc cacgcgatcc gtctcgattt tcatccgtcc gccgcggcca
     4441 tagccctgct ggcgccgcca taattcacgg ttgatcgcgt ccaggctgat gggcactccg
     4501 gccggcatcc cggtcaaagt tgcgaccaaa cattccccgt gggattcccc ggctgtttca
     4561 aaacgtaaca tgtggtggtg aaatcaatat ggtaacgaac tctcgccgta tcagcaactt
     4621 ggggtatatt tcgatcatat ggtagaacac gaaatgactc tgcgcgtgcg ctatgccgaa
     4681 accgatcaga tgggcgtggc ctattacgcc aactacctcg tctggatgga attgggccgc
     4741 gtggagtact gccgcgcagc cggactgcga taccgggaca tggaggaaaa cgaccaggtg
     4801 ctcttagccg tagtggaagc gagttgccgg tatgtctccc ccgcccgcta cgacgacgag
     4861 gtcatcgtga aaacgtggac ggagcattcc actcgccgca cggtgacatt tggctatgag
     4921 atgcgcatcg ccggctccaa tcggaaaatc gcgacgggcg agacgcgtca cgtgttttgt
     4981 gggccggata tgaagccgcg aaaacttccg gaaaagtacg gggaactctt cggggtcggg
     5041 atcaacggct agccccggac cctactccag cggcagcgcg gaaacgaagg ttttacgcgg
     5101 gtacttcacc gtaattttga acttctcacc gagccccaga ttcccggcga cggacagaga
     5161 gtagtgattg cgtatcacct caaaaatacg cgcggaagga tcgtccacgt gctcccgttc
     5221 catctcacgc aggctgaacg gcgctcccgc cgtctggcgc gcgaggctct cgaacagact
     5281 tcgcccgtag ccgcctacga aaacaggata aatcgatacg ccattttgcc gggccagccg
     5341 gaccacttgc cgctccgaca tgcggctggg accttccagg cccgtcgtca gcaacaggat
     5401 gacgcgccgg aaattgctgc tctgaaaccc gccatctatc gccgcataca gggcgtcgag
     5461 catgcgggga gagttcccgt atttcacgtt cccgagagcg cgcaacaatg cgtcgcgact
     5521 ggacgtgaaa tcctgaatca gatcggccga ggagtggaac gaaaccaggg ccatctgttc
     5581 cttttcctga agttggccga tcagtttcgc ggctaccggc tgaactacgg gccctaccag
     5641 gctggtgtcc accagcagca tgatatcggc cattactttc gtatattcga ccgattcgac
     5701 cttgcgctca actttatcgt cactgaccgt gaaatccaat cctttcaatc cggtaaccgg
     5761 cgctcccgtc ttctgctcca cgacagtcac tacgactttg gtagcgccgc agagcggaag
     5821 aaccgccagc atcgccagca tggaaagaga gcgcagcata tcgagaatca gtccgaagtt
     5881 cgagggttgt cagccgtgtc ggtaacccgc actttcacac cggaatcaag ccgggaaagt
     5941 tgggtaatgg cgacctgttc ccccacattc agaccctcca gcacctcgac cgattcgtcg
     6001 aaccggtcgc caagcttgat ctcgcgcgca tcgatcgagc catccttcac cacataggct
     6061 ttgttggatc caagcacata attgacggcc cgcaccggaa ccagtttgac ttcgtcgatt
     6121 ttatcggtgg gcactctcgc cttggcatag aagccgggct tcaactctcc gcgcggattc
     6181 tgaatcaggg cctcgacgac aaatgtccgt ttgctctggt ccaccgtggg cgagatccgc
     6241 cataccgtgc cgttgaagat ccggtcgccg aaagcctcca cggacaccgc ggccacctgg
     6301 ccgttcttga tccacggcgc catacgttcc ggaatttcca cgcgcagccg gatcgggtcc
     6361 accttgacca gcgtgatcag cggggtgttg gcacggacat actgcccgac ggttacctgt
     6421 ctttccttta cgtaggaggc aaaaggagcc cgcaccgatg tatccctcag ctttttgcgc
     6481 tgcaactcga cagcagcctt gaaccgttca acttcctgaa tcagattccg tgtctggttg
     6541 atgctggcgt cgtaagccgc ctgtgccgcc ttgaagcggg ctaccgcctg gtccaggtcc
     6601 gcatgtgctc caatgccctg gtccaccagt tcccgaacac gtttgtgacg ttgttcggca
     6661 tcgaacaggt ccgcctgcgc accccgcact tccggcgtct cccgtatgtc cttcacccgg
     6721 tctttttcgc tggataatcc cagcctctcg agcgactgcc tgagctgtgc ttcgttttgc
     6781 gaaagcagat actgctgctc ctcctcggag atgtgcacca gcaactggcc ttgcgccacg
     6841 ctgtcgccca agtccaccag aacccggtcc acccggccat ccacttctgc actgatcaga
     6901 acttcatcgt aaggaaataa cgtcccaacg gattccacaa ttcgctggat tttttttaca
     6961 ccgacggacg tagcacggat cgatatgggt ccagaatcct gctttgcctg aatagcaggc
     7021 tcccggccgc aaccggccgc taatgccgag cagatggaaa cgacgaacac tatatatatt
     7081 tgggggcgca agcgcatgaa accttgttcc tggagcgaga gaaatatcag tttatcaata
     7141 ttatggcgat tgcaggctca acgtggttac cgcctatccc ggcgattcct caggactccg
     7201 tgctttccac gagtttctca acgttaacgg tgtagagggc gggcttgccg tgacgatcgc
     7261 tttcaaaaaa cacctggcgg ctgttgggag aaaagatggg cgcggtccgc tccggcatcg
     7321 ttgacttgtg ctcgcaaatc gtgagttcac ggcgcacggc gcggatcagc agcaaaatat
     7381 acggtgaggc tctgtttgcg ctcgctccca caaatacgct tgcgtcggta tttcgggcga
     7441 acgccacgaa ctggctcgtc tcggcgatca atccgtccgt atccgactcc ggcgtgaact
     7501 cccgcagaac attcaacctg gatctgtcct ctggagtgtt gagatacagc agcgtttttc
     7561 catcgggcga ccacgcctgc cgcccgttct ttccggcagc gagtggcagc ttgcggttgt
     7621 ttctgccggt gtagtccgtc agccacgtgg agccgtcgtc cttgcggtac aaaatctcct
     7681 gcctcccggg tctcgccagc ggcagggaga taactccctc cgactgcact gccagggaag
     7741 gagacccccg gcgcagcggt accactataa gccgtgagct tccatccgta ttctcgacca
     7801 ccatcgcgtg cgttccgttg gcggcgatgc tcaacgcggc gtctccgcgg tgatagcgcc
     7861 ccggcacttc ataaatacgg cgctcccgca gcttggaaag ctcgacctgc atcagtgccg
     7921 gaccgtcgaa atagcaaaag ctcttctcat cgggcatcaa cacgagggac aacggatcca
     7981 ggaactccgc ctccgtcaac tggctgcttt ctcccgattt cagatccata cgaaagacct
     8041 gcggtgagcc cgtccggtcg caggagtaga gcagaaagtc gctccgccgc gagatcgatt
     8101 tcagcgacga cgatgttaag tggctggaat gctccggatt ggtcaaccgg acgacaggaa
     8161 attcggtgga cgggtcggga taccaacgct tttccgaggg aagaagctct cttttttcga
     8221 gttttccggc gaaggctgga aacgcaacgg ataacaaaag gccgcggcgg ctcaagccgc
     8281 gcaccagcgg cccggggacc tgcatacccc gatcctacca cgcctatttg tggccggacc
     8341 ggtagtgctc tttcagcaga tccgcgcccc aatcaccggt gaaatcgcgc cagaccgaat
     8401 gaatgtggtt cgcgttgttc tgcgtgttgt cgtactcgat caggaacgac ggcgcctgga
     8461 tgcgatagta atgcggtccg ccgcgctcaa tgctgccggc ccatgcaaaa tggagatcgt
     8521 tgccggtctt tttgatctgc tcgatgcgcg cctgggcggc gtcctccggc aggttgtgcg
     8581 catactcggc aagcagcacc tggagcaatt cccgttgctt ccccgacatc ttcgccgcgg
     8641 agagccccgt aggctggccc ttcagggcgg cttcgcgatc cgcggccgtc aggatatcgg
     8701 gataagcggt cttgctcaca atcgcggtct ggcgctgagt ggaatccaat gccgatatca
     8761 cgtccctccc gaggtcttct tcccgcgcca gaatgcgaag cccttccatg ggaccgctgg
     8821 gtacgcgcgc cggattggcg cccagaaaca gcgggcttcc cgatattttg ccgttcacca
     8881 ccgtgtagtg caggctgagg tggtggccct ccacgcgata gccccacggc tctttatccg
     8941 acggatcccc gaatatgctg aagaagtatt tctcaggatc gcgtcttccg gacggcgagc
     9001 gctcgatttg ccgcaacacc tcttcgaggc tcataatcgt gctcgccttc acatacccgg
     9061 actggctcag cccggcgctc aggagggcat gcgcgaggtg ccgctgcatc ggcgtcatct
     9121 ccagaaacgg gacgcccttt cggggccggc catagcgttt tgggatgtcg tcggacggga
     9181 tgaagtgcca gaagaaccgc tcgtcatcct ggaaagcaaa acgggttttg gctacctgct
     9241 cggcactcag ggaattgagg aacgctctgg ccgtctgtgc catggtatcc gccgaatgga
     9301 ttcgcttgta agccgacgtc agtaggatca ttccaagccc cagggctaaa atgcgccatc
     9361 tgttggcgtt caccgttagc acccctttcc gttgcggcgg tcatgctccc aggccgcaga
     9421 actactctat accagcagtc ggtatggatg taaagcaatc ccctgaacag gggaacccgg
     9481 gctgcgacct tcatgggatg tgtaatgcgc agagcctgcc atagacttag ggcatgtaca
     9541 gtggacaacg gcccgaagca atcgactcgc ggtgggtctt ccggatttac gccgccatgg
     9601 cttgcctcac cggattcctt cttaccggtt ggggcttgat ttggttcggc gccgacatcc
     9661 ccggccaacc ctggggcaag gcggcgttaa tccgggtctt tggttcgatc gtgatggccg
     9721 ctggattctg tgccgccggg tttgcagcca tcgatcagcc gctggcccgg cggcgtggct
     9781 tgttttggtt cgccaccgca cacgccgtcg tctggctcgt cgtgctcaca caacagatag
     9841 caatttggga ctccgggctc gcagagaaag tcacacagtt gctattgtgc ccagttctca
     9901 ttttttacta cctgtgggcc acgccgacag gtgaatccgc gtaccggatt ctgatcggtt
     9961 tgtttgaggg taaccgtcaa agttcggcgg aacaactgcg ttcgcagtat gagcggcaga
    10021 ttcgcgaagt ggcaaagcag gaagagcgga accgcctggc acgggatctg cacgactcca
    10081 tcaagcagca gattttcgtg attcagacag ccgcggcgac cgcacaggcc cgattcgacg
    10141 gagatcaaac cggcgctaag caggcgctgg atcagatccg cggttccgcc cgcgaggcga
    10201 tgacggaaat gcaggtgatg ttggagcaac tgcgggcagc gccgctcgaa aacaccggcc
    10261 ttgtcgaatc cttaaagaaa caatgtgaag cacttacgtt tcggaccggc gcacgagcgg
    10321 agttcaagct cgggaatctt cctgcggcgg agatgcttcc gcccggagcc cacgaagcga
    10381 ttctgcgggt tgcacaggag gcccttgcca acatcgcccg gcacgcccgc gcaggcagcg
    10441 tggtggtgtc actcggttcg gtgcatgggc acatcgaact gaaagtccag gatgacggag
    10501 ccgggttcga cccaaaccaa agcgacgggg gccagggcat ccggaacatg aaagccagag
    10561 cggaagaatt tggcggtacg ttcgagctgg taagcgggcc gggcagcggc acatccgtag
    10621 cgcttgcgat cccattcaca gcggctgaga cgccgcgagt gtaccggcgc aaggcggcgg
    10681 catgggcctt catgctagtg atatcgattg cgattctctc ctggacaagg ccaatcccaa
    10741 tagccgtcat cgccatcgcc attgtaggat tcgcccgcta cctcgttgcc taccgccgtg
    10801 cgcaaatgcg aagcgaggcc gcgcaatgac tcctgtgtct atcgcgttgg tggacgacca
    10861 ccgcgttgtc gcgcatagcc tgaaggcgta tctggagtcg tttccagatc tcagggtcgt
    10921 tggcatcgca accagcggcg aagagttgtt gaggcggatt gatgattgga aaccccaggt
    10981 cgtattgcag gatttactga tgcctggcgg aatcgacggg atcgagacca tccgccgcat
    11041 ttcggaacgc gccccgggcg tgaaggtcat cgccctcacc gcatccatcg atgaagcacg
    11101 aatgatggga tctctgcgcg ccggagcggc cggatatgtt cgtaaggatg cggaaccgga
    11161 aatcctgttg gcggccgttc gcgcggtggc cagaggcggg acctatatcg atccttccgt
    11221 gagcagacag atcctccggg ccgtagttcc gcatgaagat ctgaccccgc gcgaaattgg
    11281 agtcctgcgg cagctcgcgc tgggccggtc caacaaagaa attgccgatg ctctttcgat
    11341 cggcgacgaa accgtgaaga cgcatgtcgg caacgtgttt gccaaactcc aggtggaaaa
    11401 tcgggctcaa gcgattgtgc aggcgcttaa gcgcggactc gtctcactgg aagaactgga
    11461 gcagtagctc caccgattca gaacggcaga tcttcttttc tgaattcctg cgccaccatg
    11521 gtcttcagga acgggtaagg attgacaggt gtccggttct ggcgcacttc atagtgcaca
    11581 tgcgaggatg tggcgcgccc cgtagccccg gcgcccccga cgatttcccc acgccggatt
    11641 tcctgcccct caactacatc gatccgggaa agatgggcgt agtaggtctg aagatcccca
    11701 tgatcgacaa ctaccagctt gccgtacgcg ccactgtatt ccgcatgcac gacaacgccg
    11761 tcggcagttg cctgtaccgg ggtcccggta gccgctgaaa tatctacacc ggagtggaat
    11821 agccattcgc cggagaacgg atcgctgcgg cggccgaaat gactcatcaa acgtccgtta
    11881 accggccaca tatccggacg cacgtttgtc tgccagtacg atggataccg cctgcgcagc
    11941 ttcgaaaaac ttgcaccttt caggaagtta tactcggcca gactctcgga aaacgtgggg
    12001 actagccgcc cttccgacga gatatccgtc gtgccttcca gcttctgctt gataccatag
    12061 gcgatcgaaa cttccgttgc gaatacctga agagtagcta actgctcttt cgtctggttg
    12121 gctgaattct gtaagttctg gtatctcgcc cgaagcgtgt taaattccgt gcgtaatgaa
    12181 ttgtagtctg caactttcca cgccatccgc gcataactgg atacgattcc aaacaaggaa
    12241 aaacaaccaa gaagggccag tgccagtacg ccatacacta cacgctgagg aatgtgcact
    12301 ctgcgcaggc gcccatgtac cgaatgcgca aggacgagaa taaagtagcg ctggttcata
    12361 tgttctcttg tgcctttact ctctctgtaa gtcagccaac tgcgtaaagg agcgcaccgg
    12421 tatacccggc ggcatccgta aaaagtacta aggacgaatt tactaccgta acagctctga
    12481 aaatgcggtg tcaacctgag tggtggggag gattgacgca ccaggagtgg caaaccgacc
    12541 cggtcttact tcgtatgagc gtgatcccac gcgacaaatc agtttcattt cgcatacatt
    12601 ggaacagatg acacgtcgag agcttcttgc cgccggcatg ggcgcgatgg tccggcccgc
    12661 cgccgctcag gaccgcacga agtttcaaat tgggtgcatg acacttcctt atgcgccttt
    12721 cccgctcgaa cgcgcgctgc agggcctcgc gaaggccggc tgccgctaca tcggttgggg
    12781 cacgacgcat atggagtctt ccggacagcg ggttcccgtg atgggacccg acgcgccgcc
    12841 atccgaagcc agacaactcg cgcgcaaatg ccgcgacctt ggcctggagc ccgtcatgat
    12901 gttcaacatc acttacgtcg aggcgccgga ttccattaag gttcacacca gccggatcga
    12961 gcaggccgcc gcggctggca ttccgttcat catcactttc gggaaagtga gtcccggagg
    13021 ttccggcggc tacgatgtct ggatcaagaa cctgaagcag cttggatcga ttgcgcgcca
    13081 gaacggcgtc atcatcacca tcaagcagca tggcggtaac acggccacgg ggaaggactg
    13141 ttcaaaaatc atcgcggatg tagccgacga cggtgtgaag atgtgctacg acgcgggcaa
    13201 tgtcctcgat tatcagagtg tcgatccaat ccccgatatc caggcatgct ggcgcgacat
    13261 ccgatccttc gcgatcaaag accaccgcaa cacgccaaag gacgaggact gcgggcccgg
    13321 gttcggcgag atcgatcact ataagctgct gatgccggtg atgcgaaccg ggctgacgat
    13381 cccgctaatc tgcgaaaaca tctttgagcc gttggtccgg cggccgtcgt cgccggacgg
    13441 tgtagacgca ctggcgcgcc gggcgcggga atacgtcgaa acagtggttc gcggcctgga
    13501 aagcgtctga gacaggctca gacgttcacg cccatttcgt cccaggtaaa cacgcgcttc
    13561 tggtaaatcg cttcgcggcc catgatggcg gtcagcgcag cgatcgctcc aattttcaag
    13621 tcggctgggg gctgcttgcc ggtacgaatg gcttcgaaga acgccgccat gtgcgcttcg
    13681 ttgttctctt tgatctccgg gacaagcact accggttccg ctttgcgtgc gcgcttatag
    13741 aatgtcgcgc ggttcagatc gacggcgcct tccgtgccgt agacatgcac gaactggcca
    13801 ttgctcggca aaccgttcgg gtggaagaaa acctgcgtga acgacatttt cacgtcattc
    13861 tcgtagtcat aactcagggt gtaaccgtcc atactcgttc gcccgggggg atcgttcttc
    13921 cacaacagag ccccgccgaa accgccggcg cgggccggcc ggctccctat cacccaatta
    13981 cacagatcca ggttatgcac cgccatttcg acgatcacgt ctccggagcg cttggcgtcg
    14041 aaaaaccaat ccgccgacga gccttggtga tccagatcgt ccccggcatg ccgctgggct
    14101 ttcaccataa tcactttgcc cagcacgcct tccttctgaa ttttttcgat agtttgccgg
    14161 atccgctcca tcgatcgcat ctgctggccg ggcacaaaaa ccttattcga gttccgtgcg
    14221 acttcgagca gcctgccgat cgattccggc gtgatcccga ccggcttctc acaatagacg
    14281 tgcttgccgg cctgcaaggc ggcgatcgcc atctcgacgt gaaggtcgca aggcgtcgcg
    14341 atgtaaaccg cctcgacatc tttgtcctcc agcaggcgcc ggtaatccgt gtatgttttc
    14401 ggcttgtgtt ccgccgccgc cgtggcagcc ttgtccaggc ggtccggctt gatgtcgcaa
    14461 acggcaacga ctttcgccgc cggcagaccg aggacggttt tcagcaggta cgcgccccgg
    14521 tttcccgtgc caatcattcc ggtgccaatc gcgtcaccgg atgactgccc gaacaaagcg
    14581 gttgaagacg caatcgaact catgagaaaa cttctgcggt tcatatgcta atagagtacc
    14641 agcgccgtca ccagagtggc agcgatcgag caagcgtcac ggccttcgtc cccgacttga
    14701 tcacgatcct gccatcttcg acagcaacat cgtccacctt ttcgaaatcc cgcggcatcc
    14761 gcgtataaaa caggataaag cgcgaggtga ttttggattt cgcgggcagc cagcggtaca
    14821 ccggagcgcc gaacatcgag ccctgttgta tggcttcgcg ccgcggaaca tcgtaaggct
    14881 gcgtcgaaaa ctccagtccg cgagccatct ttccagtggg cggatagttt tcccaggtct
    14941 gaagccaggg aaactcttcc ggattgaaga gccaaccggt gagcagttgc ttgccgggat
    15001 gcaaggcaac cacccatgcg tatttcgctc ccgtcatgag cgtggtcgtg tggtcgcccg
    15061 agtccggctc cgccggtgcg gcacgcaggt tcacggtgcc gcccgccgcc agcggagcca
    15121 tcggccacgt gaagtccaca ccggaagcga gccgtttcgc caaccctcgt ccgccctgcg
    15181 actgttcgta tggacgcgtt tgcgaccggc tgccggaaat atcgaccacc gtcttccccg
    15241 gctcgagaaa cggcgagcca atggtggcat gttccgccca gacggcgggg cggtcgaacg
    15301 cgagcaggct ttcgatttcc gtgtccacat agagaatgtt ctcaccgtcc accatccggt
    15361 acgtgcgcgt gacggcttcc tcgagaatcg gaagtttggc gctcatgcgc agcgttgtga
    15421 cgccgccgct cttctctgaa acgagaatct caaagccttg ccggtgagct tccccgtgtc
    15481 ctgggagacc agcggcccgc tcctcggccg acacggaacc gaatccatcc acgcacaaaa
    15541 aatgtccgag tcccgcacca ccacccgctg gccgtcccaa ttcccgggcc attcgaattg
    15601 gattccacag cggattcaat tgttcgggat cgtcccgcac ggccacgctc gcaatcgttg
    15661 cgcccgtcgt gagaatgacg acgtccagct ttccactgga tattgccagc gcgggttgcc
    15721 cttcaaatgt ggatgtttga ggcgattggg ccgcaagcgg cagaaccagg acgagtactg
    15781 caaatagggt gcgcatcgcc gttcattata agcctgcatc cgcgcggcac tggtccttat
    15841 cgcgatgctg tgccagaatg atttgaaagc tctgggccaa atagcgaaaa tgaagaacct
    15901 cgaagtggcg acctcgcgcg agatttccgc ctcgccctcc cggcagttca aatatctcga
    15961 tttctttatc tatgtgttcg tcgtagtact gctggtctcg aatatcgtgg ggccgaagat
    16021 cacggcaatc ggaccgttcc gcatcagcgg cgcacaactg ctgtttccca tcacctacat
    16081 ctttggcgac attttcaccg aggtgtacgg gtacgccgct tcccggcggg cgatctgggc
    16141 gggcttcttc gcttcggcgc tgctgtccgt catgggcctg ttcgccgtgt ggctgccgcc
    16201 cgcgcccgga tgggaaggcc aggaagcgtt cgcgcaggtg ctgggattcg ttccgcgaat
    16261 ggttgccgcc agcctcatcg catactggtg cggagagttc accaatgcat ttgtcatgtc
    16321 ccgcatgaaa gtgctgacag gcggacgcta cctctggatg cgcacgatcg gttccacggc
    16381 catcggacaa ctcgttgaca ccgcgctggt gatgactctg gcgttcggcg gaacggtgcg
    16441 cgtgagcctg ctgttgaact tgattctctc gggatatctc ggaaaggttc tctatgaagc
    16501 agccgccacc cccgtcactt acgctgttgt gaatttcctg aagcggaaag agggcgtcga
    16561 cgtgtacgac tatgcgacga actacaaccc gtttgaagga aatatcattc gcgaaacata
    16621 ggcgtcctgg aattcagggt ccgcgttcag catgacgtgt tgaacacgtg cacggagcca
    16681 ttcgaagttg tcgtccggac gaaacaacgc catcttcgct cccagcaggg aggtgtttcc
    16741 cgcgggttcc acctgctctt ccggaaaatc aatcagcccg atgcgccgcg cgctcgcccg
    16801 gttcacgtaa ttgccgaacg cgccggccag gtagatgcgt ttcagtgcgg ccggcgccag
    16861 accacatcgc gccgcgagca ggtccagtcc ggcggcaatc gctcctttgg ccagttgcag
    16921 ttcgcgtatg tccgattgct gtagcgcgat tccgggcgca agcgtaagcg ggccgttttt
    16981 caaacgcccg ctgggctgca ccaggcccgc ttccagcccg caggcaaccg catccaccaa
    17041 cccgctaccg cagacgccgc gcgccggccc tcccccgatc accgtcgaaa tcagcgcgcc
    17101 gtcgcgaatg gaaacttcgg agatggcgcc cgtcgccgct cgcatcccgg cgccgatccg
    17161 gccgccttcg aaggccggac ccgccgcggt ggatgcgcaa tacaagcggt cgcaagttcc
    17221 cacgaccact tcgccgttgg tgccgagatc catcagcgca accggttcta cggattcgtg
    17281 cagacccgtg gcaaggatac cggcaagaat atcgctcccg acgaatccgc cgaggcacgg
    17341 cagaaaacgt atacgcccgc cccgcggcca tccgagttct tccggctgaa agcattcgag
    17401 gccatcgcgc acaggctcga acgggtaatg cgatagcggt tcgacgtcga tgccacagaa
    17461 gaggtgatgc atcacggtgt tgcccacgat cactacgtcg gcaatcgcag gaacgtcgaa
    17521 acccgcgatc atcgcgccga gcccgcatcg tatgatgtcg cgaagctcct tcgcaccgga
    17581 tggtgtgagc gcgtgttgca cccggctcat gatgtcgctg ccgtgaacgg cctgcccgtt
    17641 caaagccgtg ttcaccgcgt gtacctgcgc ggtggcaaga tcgatgagtt gcgcgactac
    17701 ggtagtggtg ccgagatcga tcgcgacgcc aaaaccttcg cgcggcgtga aatgaaacgg
    17761 gcgattgtcc gtgaggatgg gggattccca ctgagcgatt tcgagaacac aatcgtcgcg
    17821 gacaacactg cggcaggcca ggcgccatcc ttgctcaatc tcttcgctgc tgagaatgcg
    17881 aagctcatcc tgaaccggcg gcagcacgcc accggttacg cgtacgcggc acgctttaca
    17941 cttcccacgt ccaccgcaag gaaattcgac tccatacgaa aaaaggactt cctttaaaag
    18001 aagtccttcg tcaatgtaga ttgcttttcc aagcggttca agacggcacc atgccatctt
    18061 ttgagcatag cccggcgcgg ggccgctccc gcacggtcac ggcttggaac ggagccgcgc
    18121 gcgcaagcaa gcggtatcgg actcagcgca ctcgttcggt caacgtcacc gggtctgtca
    18181 gcttgaaagc gatccttgtt tccacgtcga tctgagcggg ctttccgcga gtggccatca
    18241 ctccgccggt gcccgcggcg ccgccggctg ccgcaccgat agcagcgcct tttccacctc
    18301 cggcaatggc gccgatggcg gcacctaaac ccgccgcgac tccaaccttc gccacatcac
    18361 tgcccttgct cgcagggccg gatttcacga acggttctgt ctggattgcc actttctgac
    18421 catcgcgcgt gttgagtctg acaagctgaa tggagagctg ggacaggccc ttcacccttc
    18481 ccgcctcctg cgcttccacg acgcgccctt ccagccttgc gccccgctcg gcaattacaa
    18541 gctcatccac aaccagcggc tggtccagcg tggcgaaaaa ggtgtcgcca gcctggtcct
    18601 tatcgctgtt gagcgtttcg gcgagccgca cattaatgag agtgccggac ggaatcgtta
    18661 ccttccgcgg tggaggcggt gcggggggct ctatcttttc cggctgtagg atggccggag
    18721 cgggcgccgg ggccggaggc tcgaccggat tgacaacagg agcgggctga ggagccggct
    18781 gctgcgtctg cgccacttca accggcttgg caggcggtga tggatcactg gtgcgcgcgg
    18841 gcttgcgttc acggcggcgc tccaccggag cgggagacgg cttagcggcg ggaggcgcaa
    18901 gatttaccgg ctccgccggt gcgggtgtct cgaccgctgg agattcaact accggaatcg
    18961 gtgccgcaac actggcagcg ggttcgggag cagcaggctc cctcatcatg tacacggtaa
    19021 atccggccgc caccacaact ccggctgcaa aagccagaaa tggtttcaac atgaggacct
    19081 cctcctcata tgtgttaaac gtctcagacg cgcccggcgt ttcggccaac cgaaagaacc
    19141 gaagcggcta cgcttaagtc cgcaatgaaa cgctccattt cggcgcggcg cgtctcctcg
    19201 tccggtgcac gaagaatcga cgaagggtga actgtcgcca gcataaacgg cgcgagcggc
    19261 gattccacaa actggccgcg gttctgtgta acgcgaaatt gcctgccgag caatgcctgc
    19321 gcggcggttg cacccagaca gacaattacc ttcggctgca ccatctgaat ttcggcgtcg
    19381 agccacggac ggcacgccga aatttcggcc tgattcggct tcgagtgaat gcgccgtttt
    19441 ccccgcggct cccatttgaa atgcttgacg acattcgtca cgtaggtctg tttggggtcg
    19501 atgcccgcgg aggccagagc tttatccagc aacttgcccg ccggtcccac aaagggaagc
    19561 cctttgaggt cttcgtcgtt gcccggctgt tcgcccacca gcatcacctt cgcctgcggc
    19621 gtcccggcgc cgaatactgt ctgggttccc ctcttataca ggtcacatgc ctggcagccc
    19681 gccgctgctt cgcgaagctt gccgatatcc ggatcgagcg gaatcagcgg tgcggccgta
    19741 accagtggcg tccgcgcggc catacttcga ttgtatgcgg cactttcagt agaatgttca
    19801 ctctatgcgc ccttttcatt tgctcctggt attgccgctg atgctcccgg ctcagcaggc
    19861 ccgcgaggaa agctttcttg gcagcctcgc tcccgtgatc cgctcgattc aggaagagcg
    19921 cgggttcccg atggcttatg aaaatcggaa ggggatttcg gtggacgagt ggcgccgccg
    19981 cggacgcgcg gaagtccagc gctgtctatc cttctctccg aagccggttc cgctcgatgt
    20041 gaaggttcac tctgtagtgg aacgcgacgg atacgaggtg cggaagattt ctttcgccgg
    20101 gtcgggccat taccgcgtgc cggcgtacct gctggtgcca aagaagtccg gacagaagtt
    20161 gcccgcggtt gtggcgctgc acgatcacgg cggctggttt tttcacggca aggaaaagct
    20221 ggtaaagctg gaagacgaac acgctgccct gacggccttc cgcgagcagt attacggcgg
    20281 ccggacttat gcagacgaac tggcgcgccg tggcttcgtc gtcatcgtcc ccgacgcgtt
    20341 ctattggggc gagcggcgtc tcacctacga gcagcctcca aaggagcttg cttccaggct
    20401 ggcccggcac gaccccgcat cggccgagta tgttcgggct tacaactcgt gggcgcgcga
    20461 gcggaccacc gaactgaaca cctggctttc gttcagcgga accagttgga tgggcattgt
    20521 caatcacgac gaccgccgca gcgtggatgt tctcgcttcg cttcccgaag tggatccaaa
    20581 gcgcatcggt tgcctggggc tgtcaggcgg gggctaccgg tccacttacc ttgccggtat
    20641 ggagccgcga atccgggcgg cagtgattac cggatggatg acctctctcc ccaccacgct
    20701 cgatatttcc tattccgttc atagctctct gttcgatgcc ttcggcgtgc accagaacct
    20761 cgatcattcc gacatcggga gcctggcagc acccgattgc gccgtgtttg tgcagaactg
    20821 cgcgcgcgac cgcctgttca cgcgcgccgg aatggaatcg gccgcggaaa aaattcgcgc
    20881 cgtgtacacg gatttaaaac gtcccgaacg ctaccgctcg aagttctacg acgtgccgca
    20941 ccagttcaac gtcgagatgc aggaagaggc tttcgcatgg ctggagcagt ggttgtcgaa
    21001 accctgagcc gcctctgtcg agcggggaac tctacggaac gacgcggggc atttattgta
    21061 aagttatcgt cgaaaggact actggcggaa tgatgcctat tggcatttct cgtgcgggac
    21121 gtgtcgctac gctggttctc cgggttcgct agcgttcggc gttgggtacg agttgccaaa
    21181 ggctccatag gtacacgaac atgattgccg tttcccgagc tgcaacgctg gcctgcgcag
    21241 cagtcctgac cttcgccggc gaggttccgc tggcccagca agtcgcctcg gaaatcgaac
    21301 agccatcggt ggtcttttct accactgccc ggcttgttca tgtgaacgtc gtcgccgaag
    21361 acaagcaggg ccgcccgatg gcggacctgc ggaaagaaga tttcgagatt ctggacaacg
    21421 gcgtctcaca gagcatcgct ttcttcacgg cagagcacac catcgccccc aacaggctag
    21481 ttgcggtaga aggcgtattc acaaatcaac tgcctgttgc cactggatgg cggagtggtt
    21541 actcggtcat tcttctggat tggttaaata cagcgtgggg agacaaagtc tacgcgcgaa
    21601 accaggcgct tcagatgctc acttcagttg gagtgcacga tcaggtagct ctctatgtct
    21661 tgggcaatgg gcttcgtgta gtccacgaat tcaccgcgga cacggtcgct ctggcccgga
    21721 agattgctgc cgagcagggt caggtggtgg acgctcccga tccggaggtg aaccctctga
    21781 cggacgcgag cgttggctcg ctgccttttt cagatatcgg gcctggtggt ctctccgcca
    21841 ccaagcggga ggagcgcttc tttatcg
//
LOCUS       DASTMK010000262         7524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_459659, whole genome shotgun sequence.
ACCESSION   DASTMK010000262 DASTMK010000000
VERSION     DASTMK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459659"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VFQ79_11280"
     CDS             complement(<1..1541)
                     /locus_tag="VFQ79_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140290.1"
                     /translation="MRRWWISLVLSALYVAAQQAPSPDVRAWLAEAQRQLQLRKFDAA
                     GNAARQALALEPNLAEGEALLGFVATMKGDFSEAERRFHRAVELQPRNAHLHGYLGST
                     YLQQKRLKEARSSFEAVLKLDAANQAATYNLGLIALLENRPTEALASFQAVLKTNGSD
                     AAALIGALESQLLLKQSKEAAGTTRRLETILGPGDPRLAQVAALLASHGEYRTAIPLF
                     ESLYKQGGSRDVSFNLALAHLKTGNANRAAEVLAPLLVGGDAEIFHLMATVEEARQHP
                     IEALQAYRRASELEAANEVYRFDYASALLQHETVEAAVAAFARGVRDFPRSWRMRLGL
                     GSTYYLAEQYERAAEALLEAVELEPSSELAYYLLGKAYEPAGAMQEKIRERFRQYLAR
                     NPKDAWAYYHYAMMLYQNSRASRQSVPPEVVSHLKKALQLAPDLAEAHQQLGVLAMER
                     GEMQAAIASLERAVQLDANLATAHYRLGLAYRKTGLVEKAAVEMEIFRKLKADSPAGA
                     DRGRVL"
     gene            complement(1549..3291)
                     /locus_tag="VFQ79_11285"
     CDS             complement(1549..3291)
                     /locus_tag="VFQ79_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HEU0140291.1"
                     /translation="MLAPCLPAQWVGRPAPQWEERFQVWVADAHKHVFPNSFGRDLEK
                     RAIEIEACRNEHAVIQLGLRSPEAVKSVSVRAGEFTGGGRKLGARVRVRYPGLMPVDE
                     NAQYTPDPLWEVNSVALRPYQSQGVWVDLKVPAEAQPGVYESTLQLLRDGKTMDTFRL
                     KLDVVPATLPDPADFRFFLNILFDPSSIARFQKLPLWGEEHWRRIERYIENLAAHGQK
                     TITAFIVDDPWEGDTGFAVRTIVGWRADGKLEPGKAMRLSFDFSNFDRFVTTCLRAGI
                     RDHIECWSPLVQPHADHSTITYLDTAARTERKIVLPAGSPEYKEVWGQFARAFQDHLR
                     AKGWLDKTFLAFDEISTGVLDRVIPFFHEVAPDLKLMISGGDEKGKHMAESRELAFHY
                     GYYTPDSGAELPDIPARRKAGKRTLLYTAVTPLYPNTFLFSEPLESRYLGWVIRKWDF
                     DGYIRWAWNFWPATLWDQPFYKWHSGDMFFVYPGEQGPVDSIRWEMLREGIEDYECLW
                     LARAGLDKLRSAGREVGFVAKAEKDLERAIELATLQFDRTKIPKDPVPARMEEARKLV
                     NGILREVHRLGAAP"
     gene            complement(3331..6609)
                     /locus_tag="VFQ79_11290"
     CDS             complement(3331..6609)
                     /locus_tag="VFQ79_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0140292.1"
                     /translation="MTTKALCGLLVSLFVLTTPSAIVAQVATGNIGGYVRDPSGAGIP
                     NASVTARMVEQQFTRSTETNNEGYYSFQAMPPGTYEITFESKGFQRQTQRGIELTVNQ
                     NLRVDSAMAVGAVETQVSVEATAPLVDTTSPTLSGLIDDRRMVDLPLNGRNVIGLARI
                     LPGVLNVQAPQQMSDARGGPEMNVNGGRANMNQFTFNGGFFTNPSRNTGMNYPPPDAV
                     DEVRVLTHNFAAEYGRNPGSQVTVISKTGTNEFHGSAWEFLRNDKLNARNFFSSRVPA
                     LKQNQFGAAAGAPIVRDKLFLFGSYQGLRDQREAQTVEALVPSAAERRGDFTGLSTVL
                     ENPSNPVTGRPYTTPSGQPCVANNRIDPSCISPAATNLLAFVPESPTGTVSSLAASPR
                     RGDMWMLRGDWNHTSAHRIFASYFSDHNTREGPFAAGGNIPNYISESFVQDTRHLVVN
                     DTYTFSPTLLNQATFTFLDTPSDQLQTNTIDPAELGINMPQYVPTGAVSVNVGDNFAL
                     GSGFTTRFSSRNWQFRDTVNWVRGRHNFKFGYEMLRLQFRQVFIGSPGFTFTGSQTGD
                     PVADFLLGTFDSLNVNFGVRDTDTRTWAHSGFFQDEFRVHPRVTLTLGVRYEPFLPWV
                     EKNDRINTVVPGRQSTVVPDAPPGVLFPGDIPRGLADADLNNFAPRIGIAWDVFGNGK
                     TAVRAGYGVFYESINADSLAQENPPFAGFSSVFSGRLDDPYGSVGRTPPPAETTGNFG
                     CVQISEYPGYDCPLFPLPIGGVFTGLELRTPYIQSFNLSVQHQLTPSLMFETAYAGKI
                     GIKLPALRTYNPAQFINSPVDGSPPSDQNINDRVIFEPGILGTQGFMLGNDFRSWYHS
                     FQAQVTKRFGDGLSVLGSYTLSKSIDSSSTDNLGATVANPFNLRDERGRSDWDRRHAF
                     VASWLWTPPVRFDSPLAQSLLGGWTLTGIHTIQSGGPLTFLMGDDVALDGTFGDQHAQ
                     LKPGVTVNDIVVDHSSRGAMVDRFFNTDAFVPTNQVPRGTYGNAGRGLISGPAFNSSD
                     FSILKNFALREPLRLQFRTELFNAFNQVGFTTVTTTVNSSAFGRIRAARDPRIIQFGL
                     KLLW"
     gene            complement(6693..>7524)
                     /locus_tag="VFQ79_11295"
     CDS             complement(6693..>7524)
                     /locus_tag="VFQ79_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU0140293.1"
                     /translation="FTEEGLSAGVAFGEDGVARGAMGVDAVDYDRSGRPHLLVGNFSN
                     QMLGLYRNEGKGLFVDEAPRSTVGRASLLSLAFGVFFFDYNLDGYPDIFAANGHIDEE
                     IGRVQPKVQFKQLPHLFRNLGKGSFREVTSTTGDLARAMVARGAAYGDYDRDGDLDIL
                     ISSNHGPAYLFRNDRGNSNNWLSIKLSGVKSNRNGIGAMVRVESTGGRQWRAVHSGSS
                     YCSQSDLALTFGMGSDSEAKSVEIEWPSGQKDRFGPVAANQFVLIEEGKGIRINIRQQ
                     "
BASE COUNT         1489 a         2363 c         2172 g         1500 t
ORIGIN      
        1 agcacgcggc cccggtcagc gcccgccggg ctgtccgcct tcaactttcg aaagatctcc
       61 atttccaccg cggctttctc caccaaacct gttttgcggt aagccagacc caggcggtaa
      121 tgcgccgtcg cgaggttcgc gtccagttgg accgcgcgct ccagactcgc tatcgcggct
      181 tgcatttctc cccgttccat cgccagcacc cccagttgtt gatgtgcttc cgcgagatcg
      241 ggggcgagct gaagagcttt cttcagatga gaaaccactt ccggcgggac gctttgccgc
      301 gaggctcgcg agttctgata cagcatcatc gcgtagtggt agtaagccca ggcatccttg
      361 ggattccggg ccaggtattg ccggaagcgc tcgcgaatct tctcctgcat cgctcccgcc
      421 ggctcgtacg cttttccgag caggtaatac gccagctccg aggaaggctc gagttccacc
      481 gcttcgagca aagcctcggc ggcgcgctca tactgctccg caagatagta cgtggagccg
      541 agccccagac gcatacgcca ggatcttggg aaatcgcgga cgccgcgagc gaaggccgcc
      601 accgccgcct ccaccgtctc atgttgcagc aacgcactgg cgtagtcgaa gcgatagact
      661 tcgttcgcgg cttcgagttc agaagcgcgg cggtaggcct gcaacgcttc gattgggtgc
      721 tgcctggctt cctccacggt agccatgagg tggaagatct ccgcatcgcc gcccaccagt
      781 agcggcgcaa gcacttcggc cgcccggttt gcgtttccgg ttttcagatg agccagggcc
      841 agattaaagg acacatcgcg cgacccgcct tgcttataca gactctcgaa aagtggaata
      901 gccgtgcgat actcgccgtg cgaggccagc agggccgcta cctgcgccag acgcgggtcc
      961 ccaggcccga ggatcgtttc caaacggcgt gttgttcccg ctgcctcctt gctttgtttg
     1021 agcagcaact ggctttcgag tgcgccgatg agcgccgcgg cgtcggaccc gttcgttttc
     1081 aacaccgctt gaaaagatgc cagggcctct gtaggacgat tttcaagcaa cgcaatcaag
     1141 ccgaggttgt aagtcgcggc ctggttcgcg gcatcgagct tcagaacggc ttcaaacgag
     1201 gaccgcgcct ccttcaaccg cttctgctgc aggtatgtcg agccgagata gccgtgcaag
     1261 tgcgcgttac gaggctgcag ttccaccgcg cgatgaaacc ggcgttcggc ttcgctgaaa
     1321 tcccctttca tggtggcgac gaacccgagc agcgcttctc cctccgccag gttcggctcc
     1381 aatgcgagag cctgccgcgc cgcgtttccg gcagcgtcaa actttcgcaa ttgcagttgg
     1441 cgctgcgctt cggcaagcca tgcccgcaca tcgggcgatg gcgcctgttg cgcggctaca
     1501 tagagcgcgc tcaaaaccag gctgatccac caccgccgca tgacagaatc acggagcggc
     1561 gccgagacgg tgaacctccc gcagaattcc gttcaccagt ttccgcgctt cttccatgcg
     1621 agccggcacg ggatctttcg gaatcttcgt ccggtcgaac tgtagcgtcg caagctctat
     1681 cgcccgttcc aggtcctttt ccgctttcgc tacgaagccc acctcgcggc ccgcgctacg
     1741 caatttatcc aggcccgcac gcgccagcca caggcattcg taatcttcta tgccttcgcg
     1801 cagcatctcc caacggatgc tgtccacggg gccttgctcg ccgggataga cgaagaacat
     1861 atcgcccgaa tgccatttgt agaagggctg gtcccacagc gtcgcgggcc aaaagttcca
     1921 cgcccacctt atgtagccgt caaaatccca tttccggatc acccagccga gatatcgcga
     1981 ttcgagcggc tctgaaaaga gaaacgtatt cggatacagc ggcgtcaccg cggtgtacaa
     2041 caacgtgcgc ttgccggcct tcctgcgcgc tggaatgtcc ggcagctccg cgcccgagtc
     2101 cggcgtgtag tagccatagt ggaaggcgag ttcccgggac tcggccatgt gtttaccctt
     2161 ctcgtcaccc ccgctgatca tcagcttcaa atcgggcgcc acttcatgaa agaacggaat
     2221 cacccggtcc aacacccctg tactgatctc gtcgaacgcc agaaacgttt tatccagcca
     2281 gcccttcgcg cgaagatgat cctgaaacgc gcgtgcgaac tggccccaca cttccttgta
     2341 ctccggcgag ccggccggta acacgatctt cctctccgtc ctggccgccg tatccaggta
     2401 ggtgatggtc gaatgatcgg cgtgcggctg caccaatggc gaccagcact cgatgtgatc
     2461 gcggataccc gcccgcaggc aggtggtgac gaaccggtcg aaattggaaa aatcgaatga
     2521 cagcctcatt gccttgccgg gttcgagctt gccgtccgca cgccatccga cgatcgtgcg
     2581 gaccgcaaat cccgtatcgc cttcccaggg gtcgtccaca ataaacgcgg taattgtttt
     2641 ttgtccgtgc gccgccaggt tctcgatata gcgttcgatc cggcgccagt gctcctctcc
     2701 ccaaagagga agcttctgga accgtgcgat ggaagagggg tcgaaaagga tattcaggaa
     2761 gaaccggaag tccgccggat cgggtagcgt ggccggaact acatccagct tcagacgaaa
     2821 cgtatccatg gttttaccgt cgcgcaggag ctggagtgtg ctttcgtaga cgccgggctg
     2881 ggcctccgcg ggcaccttca gatccaccca gactccctgg ctttgatacg gccggagcgc
     2941 aacggagttc acttcccaca agggatccgg cgtgtattgc gcgttctcgt ctaccggcat
     3001 caagcccgga taccgcacgc ggacacgcgc ccccagtttg cgtcctccgc ccgtgaattc
     3061 gccggcacgc acggaaacag acttgacggc ctcgggactc cgcagcccaa gctgtatcac
     3121 ggcgtgctcg ttgcggcagg cttcgatttc gatggcacgc ttctccagat cacgcccaaa
     3181 gctgttgggg aaaacgtgct tgtgcgcatc ggccacccac acctggaagc gctcttccca
     3241 ttgcggcgcg gggcggccga cccactgcgc cggcaggcat ggcgcaagca tgcagaaaag
     3301 aaccaaccct cggatcatgg cagctttacg ctaccacaaa agcttcaagc cgaactgaat
     3361 aatgcgcgga tctctcgcgg cccgaattct gccgaaagcg gacgagttca ccgtcgttgt
     3421 gacggtcgtg aaacccacct ggttgaaggc gttgaacaac tccgtccgga attgcagccg
     3481 cagtggttca cgcagcgcga agttcttgag gatgctgaaa tcgctgctgt tgaacgcagg
     3541 cccgctgata agcccccttc cagcgttgcc ataggtccca cgtggcacct ggttggtggg
     3601 cacgaacgcg tctgtgttga agaatcgatc caccatcgcc ccacggctgc tgtggtcgac
     3661 aacgatgtcg ttcactgtca cccccggctt gagttgcgca tgctggtcgc cgaaagtgcc
     3721 gtcgagtgcg acgtcatctc ccatgaggaa cgtgagcggt ccgccgctct ggatagtgtg
     3781 gatgcccgtc agcgtccagc cgccgagcag gctctgtgcc agcggactgt cgaaacggac
     3841 tggcggcgtc cacaaccacg atgctacaaa cgcgtgcctc cggtcccaat ccgaacgccc
     3901 gcgctcatcg cgcagattga atggattcgc caccgtggcg ccgaggttat ccgtcgaact
     3961 cgagtcaatg gacttcgaca gtgtgtacga gccaagcacc gatagcccat cgccgaaccg
     4021 cttggtgacc tgcgcctgga agctgtggta ccagctccga aaatcgttgc ccagcatgaa
     4081 cccttgcgtg cccagaatgc caggctcgaa gatcacgcga tcattgatgt tctggtcgga
     4141 cggaggcgag ccgtccacgg gcgagttgat aaactgagcg ggattgtagg tgcgcagcgc
     4201 cggcagttta atcccgatct tgcccgcata tgcggtttcg aacatcagag agggcgtcaa
     4261 ctggtgctga accgataaat tgaacgactg gatgtacggt gtccgcagtt caaggccggt
     4321 aaacactccg ccgataggaa gcgggaacag tgggcagtcg tagccgggat attcggaaat
     4381 ttgcacgcag ccgaagttcc ctgttgtctc agcgggagga ggcgttctcc caaccgagcc
     4441 gtaggggtcg tccaggcgcc cgctgaacac actcgaaaag cccgcgaagg gcggattttc
     4501 ctgtgccagc gaatccgcgt tgatgctttc gtaaaacacg ccgtagcccg cgcgaacagc
     4561 cgttttgccg ttcccgaaca cgtcccaggc gattccgatt ctcggcgcaa aattgttcaa
     4621 atccgcgtca gccagtcctc gcgggatatc gcccgggaac aatacgcccg ggggcgcatc
     4681 cggcacaacg gtcgattgcc tgcccggaac aacagtgttg atgcggtcgt tcttctccac
     4741 ccagggaagg aacggctcat agcgcacacc cagcgtcaat gtcacgcgcg gatgcacgcg
     4801 gaattcgtcc tgaaagaatc ccgagtgcgc ccaggtgcgg gtgtccgtgt cgcgcacgcc
     4861 gaagttgaca ttcaaactgt cgaaagtgcc cagcagaaaa tcggcaaccg gatcgcccgt
     4921 ctgtgaaccg gtgaaagtga atcccggcga gccgatgaac acctgccgga attgcagccg
     4981 caacatctca tatccgaatt tgaaattgtg ccggccgcgc acccagttca cggtgtcgcg
     5041 gaactgccag ttcctgctgg agaaccgtgt cgtaaatcct gaacccagtg cgaagttgtc
     5101 tcccacgttt acggaaacag ctcccgtagg aacatactgc ggcatgttga tgccgagctc
     5161 cgccggatcg atggtattcg tctgcaactg gtcggaaggc gtatcgagaa acgtgaatgt
     5221 ggcctggttc agcagcgtcg gagagaacgt ataagtgtca ttgactacca ggtgcctggt
     5281 gtcctgcaca aagctctccg atatgtaatt ggggatgttt ccgccggcgg caaacggccc
     5341 ttcgcgcgtg ttatggtcgc tgaagtaact cgcgaatatg cgatgtgcgc tggtgtggtt
     5401 ccagtcgccg cgcaacatcc acatatcgcc gcggcgcggg ctggccgcga gcgagctcac
     5461 cgtgccggtg ggcgattccg gaacaaaggc gagcaggttg gtagcggccg gactgatgca
     5521 gcttggatcg atgcgattgt tcgccacgca gggctggccc gacggcgtcg tataggggcg
     5581 gccggtcacc ggattcgacg gattctccag caccgtggac aaccctgtga agtcgccgcg
     5641 gcgctccgcc gcgctcggca ccagcgcttc cacggtctgt gcctcccttt ggtcgcgcaa
     5701 gccctgataa gagccgaaca ggaaaagctt gtcgcgcaca atcggcgctc cggctgcggc
     5761 accgaactgg ttctgcttca aggccggaac gcggctggaa aagaagttgc gtgcattcag
     5821 cttgtcattg cgcaggaatt cccatgccga accgtgaaac tcgttggtgc cggtttttga
     5881 aatcacggtc acctgcgaac cgggattgcg gccatattcg gcggcgaagt tatgcgtaag
     5941 cacccgaact tcgtccacag cgtcgggagg cgggtagttc atgccggtat tgcgggacgg
     6001 gttcgtaaaa aatccaccgt tgaacgtgaa ctggttcatg ttggcgcggc cgccgttgac
     6061 gttcatctcc gggccgccgc gggcgtcgct catctgctgc ggagcctgaa cgttcaggac
     6121 gcccggcaaa atgcgcgcca gcccgattac attgcggccg ttgagaggaa ggtccaccat
     6181 gcggcggtcg tcgatgagcc ccgaaagagt cggcgacgtc gtgtccacca ggggcgctgt
     6241 cgcctccacg ctcacctgcg tctctacggc gcccaccgcc atggcggaat cgacacgcag
     6301 gttctggttc accgtaagct ctatgccccg ctgcgtctga cgttgaaagc ccttgctctc
     6361 gaacgtgatc tcatacgtgc cgggcggcat ggcctggaag ctgtaatagc cttcgttgtt
     6421 ggtttctgtg gaacgcgtga actgctgttc caccatgcgg gcggtgacgc ttgcgttggg
     6481 tattccggcc ccgctgggat cgcggacata accaccgata ttgccggtgg caacctgcgc
     6541 gacgatagcg gatggagtgg taaggacaaa cagcgatacg agcaatccac agagagcttt
     6601 tgtggtcata aaatggacac ttcgacgaag cccctcttcg cctctgcgtc tattgttaat
     6661 cccgaccgct acgccctgtc aagaaatttc acctattgtt ggcgaatgtt aattcggatt
     6721 cccttccctt cttcgatcag cacgaactgg ttggccgcca ccggtccaaa ccggtctttc
     6781 tggccgctcg gccattcgat ctctaccgac ttcgcttcgg aatcggaccc catcccgaat
     6841 gtcagcgcga gatcactttg cgaacagtag ctcgatccgc tgtgaaccgc gcgccactgg
     6901 cgtccgccgg tactctccac tcggaccatt gcaccgatac cgttgcgatt cgacttcacg
     6961 ccggacagct ttatcgacag ccaattattg gaattgccac ggtcattgcg aaacaggtaa
     7021 gccgggccgt ggttgctaga gatgagaata tcgaggtcgc cgtcgcggtc gtagtccccg
     7081 taagccgcgc cgcgcgccac catcgcccgc gccagatcgc ccgtggtgct tgtaacctcc
     7141 cggaaggacc ccttccccaa attgcggaac aagtgcggca gttgcttgaa ctgcaccttc
     7201 ggctgcacgc gcccgatctc ctcgtcgatg tgcccattgg ccgcaaaaat gtccggatag
     7261 ccatccaggt tgtagtcgaa aaagaacact ccgaaggcca ggctcaacag gctggcacga
     7321 cctaccgtgg agcgcggtgc ctcgtcgacg aacagcccct tgccttcatt gcggtacagc
     7381 ccaagcattt gattggaaaa gtttccgacc aaaagatgcg gccgcccgga gcggtcgtaa
     7441 tccacggcat ccacccccat tgcgcccctc gctacgccat cttcgccgaa cgcgaccccc
     7501 gcggaaagac cttcttccgt gaat
//
LOCUS       DASTMK010000263        39273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_620690, whole genome shotgun sequence.
ACCESSION   DASTMK010000263 DASTMK010000000
VERSION     DASTMK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 39273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39273
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620690"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1293..1565
                     /locus_tag="VFQ79_11300"
     CDS             1293..1565
                     /locus_tag="VFQ79_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140294.1"
                     /translation="MSSAKLQQYVAMIEHELAWARLKIQAPQQELRLERNKKYGSSSE
                     TLSSWQAELLDFRLSVSGEAAQSKSGAKPWPSSLLANANRIPGGRL"
     gene            complement(1644..2492)
                     /locus_tag="VFQ79_11305"
     CDS             complement(1644..2492)
                     /locus_tag="VFQ79_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140295.1"
                     /translation="MRSDFRPLLCAVLLIASAQAMEAQAIARLSSQPPLSPPEVAELE
                     TRVQSEPGDIATRSRLLTHYRDYMNPVFRAVRLRHILYLIEHSPASDVAASALAYVYA
                     SGGPYADAADHELARNAWLRAADANRTDASVQVNAARFLYVEHPDDAEELLRRLVEQE
                     PSNRVLASHLGFFYAMDILGMNGPFGTRGRRPSEAERLGAQARGELERTTNPLVLAAA
                     ATALPNLFMRSRARRAPGGDPTVFQYSSTLMARARQLAPGEPELRGPMPLIREFGQLT
                     GSESDR"
     gene            complement(2512..3594)
                     /gene="nrfD"
                     /locus_tag="VFQ79_11310"
     CDS             complement(2512..3594)
                     /gene="nrfD"
                     /locus_tag="VFQ79_11310"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU0140296.1"
                     /translation="MKQTFDGRPVDRVQTDGRDINTTLAELTGEGAQQVTSMSEAHLA
                     PTGVAWEQLPVPRAGDPTYYDRPLLKQSVWKPFIPLYYFTGGAAGASLVLGAAAQLDG
                     SQELDRFIRRCHWIGIIGSTISGGLLIADLGRPERFLYMLRVFRPTSPMNMGAWILAA
                     APSAAITAGLFARHSHGILQRIGEAAGYSSGAFGAALATYTGVLAGNTVIPIWKESRR
                     ILPVLFGASAAASAGAIFDLMFEDERACRITRYFGIGGRLAELAAAHSMEREAARVPT
                     VARPLKRGLSSVMWKTASVLTAASLVTLMLPRQNRKKRMVAGLLGAGGSLLMRFAVEV
                     AGNASARDPRATFHQQRAGQGTREVE"
     gene            complement(3591..4388)
                     /locus_tag="VFQ79_11315"
     CDS             complement(3591..4388)
                     /locus_tag="VFQ79_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0140297.1"
                     /translation="MAGAKGFFTDTTLCIGCKACEVACKQWNQLPDDGLLFTGMSYDN
                     TIHLGASTWRHVAFIERPVPLSTQDAGAFSWLFSSDVCKHCARAGCLESCPTGAIIRT
                     EFDSVYVQPDICNGCGYCIVNCPFGVIDRRNEDGRAWKCTLCYDRLREDMTPACAKAC
                     PTESIQFGDLDELRTRARARVEKLQDMGMREAYLYGEDAASQPGTEGLNAFFLLVDKP
                     EVYNLPPDPVIPTKEVGRSWASMAAAAAGIALMAVGSVFFSKLGGRS"
     gene            complement(4391..7660)
                     /gene="fdh"
                     /locus_tag="VFQ79_11320"
     CDS             complement(4391..7660)
                     /gene="fdh"
                     /locus_tag="VFQ79_11320"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7085..7087),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEU0140298.1"
                     /translation="MPGEYGFSLSNWPLFRQLKQRDLFGLERTAASPRTEALTARTTS
                     ADRIVSSICPYCAVGCGQLVYVKNEKIIDIEGDPASPISHGCLCPKGAATFQLVTGSH
                     RVQHVLYRRPHGKEWETIPLEQAIDMVAERVKRTRDETWEDTDEEGHPVRRTMGIAHL
                     GGATLDNEENYLIKKLFTALGIVQIENQARIUHSSTVPSLGTSFGRGGATTFQQDLQN
                     ADCIVIEGSNMAECHPVGFRWVMEARERGATIIHVDPRFTRTSAVANIHVPIRAGSDI
                     AFLGGIVRYIIENERYFRDYVVHYTNAPAIVSAEFRDTEDLDGLFGGWDEETGQYHEK
                     TWQYEGVDPEPAAGARESDEEDQTIQRNPIQAEHTDPTLQHPRCVFQILKRHFSRYTP
                     EMVEQTCGIPKSLFLKVAETLCRNSGRERTSAFCYAVGWTQHSMGVQYIRTAAIVQLL
                     LGNIGRPGGGILALRGHASIQGSTDIPTLYNLLPGYLPMPKAKHDKDLTTYLALNTSP
                     SGWWGEFPKYFVSLLKAWFGEAATRDNDWCYDYLPALTADHSHMNTVVNMADGGIKGY
                     FVMGENPVVGSMNGPLHRKGLRRLEWLVVRDFSVTETADFWRDAPEIERGEIRTEDIG
                     TEVFFFPAAAHVEKDGAFTNTQRLLQWHYKAVEPPGDAASELDFIYKLGLRLKKLYAG
                     EYDQPRNRPIRDVTWDYPLEGPKQEPKAEAVLHEINGYTVADGKPVEGFTSLKDDGST
                     ACGCWIYSGCYRDGQNMTARRKPHWEQNWVAPEWAWAWPHNRRLLYNRASADPEGKPW
                     SERKKLVWWDQEKGEWVGHDTPDFIVKRPPWFHPSKDARGKDTIGGTDPFVMQADGKG
                     WLYAATGLQDGPLPVHYEPQESVVKNPLYGQQCNPERMEWWRKDNPYHRAYDDPRFPY
                     VITTYRLTEHHTAGAMSRWLSWLSELQPEMFCEVSPELAAERGLKNGGWATIRTMRAE
                     IEARVLVTSRMRPLRIKGRLVHQIGLPYHWSTKGLARGDAANDLIGFVADPNVSIQES
                     KAFTGDIEPGRKSRKRSAATSGPPVREIDPKRWQRDLPPARRKPESQHGLKAEESKEG
                     H"
     gene            complement(7701..9443)
                     /locus_tag="VFQ79_11325"
     CDS             complement(7701..9443)
                     /locus_tag="VFQ79_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU0140299.1"
                     /translation="MNRRTLLGLLTIPALNAFGQGVASRNVKPTRRGKPSGLPFHARF
                     TDVAKQAGLTAPTIYGGVEQKPYILEAVGCGCAFLDYDNDGWLDIFMLGGTRLEGAPP
                     HAGNRLYKNNRDGTFTDVTDKAGLKRTGWASAVCIGDFDNDGHDDLFVTYFGQNVLYR
                     NNGDSTFTDVTEKAGLLQANPRWSSGCTFVDYNRDGLLDLFMGSYLEFDFKSVPKPGE
                     NANCTWKGVPVNCGPRGLPPGALALYRNNGDGTFTDVTRESGVGSVKGSYAMTAVAAD
                     FDNDGWPDIYVACDSTPSFLLRNQHDGTFAEIGLESGVALNEDGMEQAGMGLGIGDYN
                     LDGALDIYKTHFSDDTSILYRNDGKAMFDDMTIRAGLGVETRFTGWGAGVVDLDNDGW
                     PDLFHVTGSVYPEVEKKVPGYPYKTPRVVFRNLGNGQFEQFIEESGPGVAAAHPSRGC
                     AFGDFDNDGDLDVLIVNLNEPPSLLRNDISGDNQWLKVKLIGTKSNRTAIGAKVIARY
                     GGRMQVQAVLSQSSFYSVNDLRLHFGLGKATSADLEIRWPNGGAESFKNVAAGRMVTI
                     KEGEGIVSEQKFSR"
     gene            complement(9440..10774)
                     /locus_tag="VFQ79_11330"
     CDS             complement(9440..10774)
                     /locus_tag="VFQ79_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0140300.1"
                     /translation="MRRVAALLLLAASLPAADDRLQQRLEWFQDRKFGLMLHWGAYSQ
                     MGVIESWPLVWADRKWSNPKITTREEMADFRKRYFALPRTFNPTEFDPAVWARHAKAA
                     GMRYVVFTTKHHDGFAMFDTRQSEYRVTAPAVPFSRNPRANIVREVFDAFRKQGLGIG
                     AYFSKSDWHSPYYWRPDVFAEDRNPNYDTLTDSRRWAQFVQFVHGQIGELVTGYGHID
                     ILWLDGGQVRPPKQNIDMDRLAAMARRHQPGLIVVDRTVGGKHENYRTPEQKVPEKPL
                     DGPWESCITMGTQWSYKPGDTYKPTRELVHLLIDVVAKGGNLLLNIGPQPDGQLPEEA
                     VARLEEIGRWMAVNGKAIHGTRAFAPYREGRIAYTRRGNTVYAFYLLEDGKRPPTQIA
                     IPAVRAGGGTRVELLGTPAPVEWSAAAQGIIVRLPDTAMGEHAIAFEITGTL"
     gene            complement(10771..11367)
                     /locus_tag="VFQ79_11335"
     CDS             complement(10771..11367)
                     /locus_tag="VFQ79_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140301.1"
                     /translation="MDYRYRIAAPGRKTGSLWEQTIVFPEGQRYFVSSDRITTVNESP
                     ALFLRIDMPGHIKHKGTDTFSEIFLSYLGRIPAKEFLDDFAPDEKFLYRRGEGKTPRR
                     FIRAYRLRDPKTGAAGPWLAGMTLDPSVVSEAWCHQRGYVCMIQEFGGRPVKPGDHFS
                     AAFLVGYFDSVEEMERVYDRYAGHTGLEVTAQGWRLVK"
     gene            complement(11784..13061)
                     /locus_tag="VFQ79_11340"
     CDS             complement(11784..13061)
                     /locus_tag="VFQ79_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEU0140302.1"
                     /translation="MKRIVFFLCLLASAHAETVPELRAGVARVDITPAVSMQMYGYRD
                     RECGPSNGTHDPLYAKALVLESGGSRMAIVTLDLGSIVSERLKDEVRAKLGITVLLLA
                     ASHSHSAPAFLPFGSAPVRDDAGARYRTELEQKIFGAIEQASTSMFPARLGVGRGSIQ
                     LGYNRLQMQNDGRSRVLYRNPERIPWGPVDPGVDLLRVEDTAGRPRALLVHYAVHAVV
                     LGKTSCKFSADFPGMMQTKVESELPGTQVMFVQGGAGDINPLLMGRSGDEAADFAIAE
                     TMGDILAAEVLKASKDVIGVEAGPIRARSELLEFRNRWDKNRKLTVGVTTVLIGKDIA
                     IATLPGEPFHVLQKTWKAQASVAHPLFYGYTYTVGGTWAGYIPDLRSAAHGGYGADVS
                     TTIEVGGGERIIQQHLINLYDLQRMWRETPGNP"
     gene            complement(13088..14452)
                     /locus_tag="VFQ79_11345"
     CDS             complement(13088..14452)
                     /locus_tag="VFQ79_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140303.1"
                     /translation="MEQQRRQFVKCVSAAIASAQFPIIGANDRVSLGVVGLGGRGTDH
                     LNYYSTFESDCRITAICDVHQAARERAAALVRKLRNYNATEYSDMRALFDSKEIDAVS
                     LPLPNHWHALATIWACQAGKDVYVEKPACHNLFEAEQMVLAARKYDRVVQVGSQGRSI
                     THKKRAMQLLRDGAIGQVYHARGLCFRRRFSIGHTPDAAVPAGLDWDRFLGPAQVKPY
                     SLNKFAYNWHWFWDTGNGDIGNQGVHEMDICLWGIGRGGWPDSVTSTGGKYVWKDDQE
                     TPNTQHAVFDFGDAQVTFDVRNLPTPPEGLVPARTRSFIGNLFFGELGYMVVDPEGVQ
                     VYRSSLARLSPEQAVSREIRRAEKYEKVLDEKAVEGDEEATKPHMKNFLDAVRAHDRR
                     KLTADIAIGTRAAAFCHLANISLRTGRTLRLSRATGKFIGAGEANALFSRDYREPYTI
                     PPLS"
     gene            complement(14505..15881)
                     /locus_tag="VFQ79_11350"
     CDS             complement(14505..15881)
                     /locus_tag="VFQ79_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140304.1"
                     /translation="MKRPWTAAPVLAFLFLGGASGALWGQRWVNPHFDTHRMDYRDLG
                     YPAQNLIPADNSRISALLAHSNGFIYGATSGRTQSYLFFYNRFINKVRPLGKIADARG
                     VYHGLVEGKDGEIYIGTGLSMYSPVELTKEFPVEVEGIEKQLWKDITLPYRDYAGGHI
                     YRYDPKTGDVERYTNDSPAKVEDLGVPVSHNTVYAMTMSADKSKIYGITYPDAHFFIF
                     DLATRTTKDLGELLAHKVYSGPERHWRSVPRALYCDPKSGDVYTSGDNGIIVRYRPGS
                     GKIEQTFIRLPGEYWEGLSSYDYPVVEAFTTDSSGNVYAGTNDGHLVRLDIGKDDVAV
                     LGKPRIMRRLRAMAAGKDNNLYLITGEFERSCKLHTWDLSGKKGFSELGPFAVDRSPY
                     YSHRAYQFDAIAVAPDGAVFCGESDRGGKLFIYLPGPGRFKEMLNPFNPESERQRSDT
                     PGLIPERL"
     gene            complement(15892..17172)
                     /locus_tag="VFQ79_11355"
     CDS             complement(15892..17172)
                     /locus_tag="VFQ79_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140305.1"
                     /translation="MFRAMFAGFILLCSTLSCFADDRAKFVPIKHYRFSTVDKDAQMW
                     SALYIGSDGKIYVGLCTHADAANVYRFDPATGEMRHLANLTEVSEERGKGIWTNGKIH
                     VQMQELDGWVYFGSLSEDNGPPAIDAASYKGPRWYRINMKTGKVEQLGLINSFWGLLG
                     QTMDPKRRLIYGLAENGHLYKYHIDKDWTEDLGRVDSWDVCRTIFSDDLGNVYGSFPP
                     GRIWKYDIQQDRIFDFEHIRLPLINQSRTMANPMLDRKAQWRIIEWDPLDRAAYGIIG
                     GSNLLFKYDPHKGQEGTVSPLVSMAAPGVRDGDPMNLPYATLAMALSQKERKIYYITV
                     MAGDFDYGAVTKDVMGASFLVTYDLKSGRREDVGVLRTADGRTASGMGGAQIDKDGRL
                     WFVGAFKEPDPRYRAGRGRRAYSMGLGCYSPSGK"
     gene            complement(17265..18791)
                     /locus_tag="VFQ79_11360"
     CDS             complement(17265..18791)
                     /locus_tag="VFQ79_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEU0140306.1"
                     /translation="MKTSAIVMLFAAAAAAQTFDVRAHGAKGDGVTLDSPAIQKAIDA
                     AFSAGGGVVVLPAGNYLSGTLVLKSNVSLRLYPGATLWASKRAEDYNPRRLIYAVDAE
                     NIAVEGQGTINGNGDTWWDKQTGRRSYKAMPGRPSPLLEFVNSRNVRVQDVSIRQAPG
                     WAIHPLECDGVLIRGIRIIADPRGPNTDGIDPDSSRNVLIADSFIDTGDDAIVIKTTG
                     QRGGKILPSENITITNCVLTTRCNAFKMGTESRGDFRNISVTNCAIYGKPDTVQRPIS
                     GIAVEMVDGAVTDGVVVSNITMRDVDTPIFIRLGNRGRGQAVPTTGSLRNVSINNVIA
                     AGGTMSSSVTGLPGHRVQNVSLTGINITMKGGEQEVRGLLDVPEQEDKYPEARMFGKL
                     PSHGFFVRHVEGLTMRSLQLRTEEPDVRPAMIFHDVRELDLDGFRADSVHGKEAVLWF
                     HDVVGAMVRGARTPHAVQDFLRVTGAASRSIQLSNNQVAPAGRMVRIGEDVGDGAVIE
                     "
     gene            complement(18802..20328)
                     /locus_tag="VFQ79_11365"
     CDS             complement(18802..20328)
                     /locus_tag="VFQ79_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEU0140307.1"
                     /translation="MKASWLMCLVCWAAAAAAAQQAPPGVFDVRAHGATGDGKTNDTR
                     AIQQAIDACARAGGGTVHLPAGTFLSGTLVLKSNVTLYLSGGATLAGSRKIEDYQPNH
                     LIYAKGAENIGIEGPGRIEGNGDAFWYPDFKPHKRRPSPLIELVDCRDVRIRDVRIRN
                     TPGWGIHPLNCERVEIRGISIISPMQGPNTDGIDPDSSRHVIIADSYIETGDDCIVLK
                     TTGRAGMPARACENVIVNNCVLVSDDAALKLGTESHGDFRHIVFSNIVIRKTRVGLAI
                     YAKDGGVFEDVSFSNISIETFPTHPNSAEYPIYIDLEKRFPDSRQSRVRRINFSDLML
                     ATKGRVLVGGMPDQPVEDVAFRGVTMRVTGFEPVEKMSKPRGSSNVGRVEPEADYSSV
                     PAAWVFANVRGLHLRDLKLVWAADAPLLERHAVYAKGVEDLIVSGFHGRQAKPDGSLA
                     VFGLDHAKNVFISGSVAAAGAGAFASVRGVSADELVLEGNDLRNARERLGGRTHIHGK
                     "
     gene            complement(20341..23772)
                     /locus_tag="VFQ79_11370"
     CDS             complement(20341..23772)
                     /locus_tag="VFQ79_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140308.1"
                     /translation="MHIRKYLLAGMAGLCFLASASGQVNKSNLTGVVYDSSGSAIPGA
                     KLRLVNTATGLVRETGSDVTGLYRFALLDFGTYRLEIESGGFKKFVRDGILLETGETT
                     TLNATMEVGELTESVTVTGESPLLRTETGALGTSVNTQVIAELPLISRNPYAFLTLAA
                     GVQHMRGSADINQWDNNGPSDFAAGGSESSSEFLLDGIPNMRIQTVSFSPPPEAVQEM
                     RVQTNAFDAEYGHSGAAFVNVSTRGGTNELQGSVYWFHRNDNLNANSFFNNRNGNPKA
                     ERKRNTYGFNVGGPVYLPKLYDGRDRTHFHTNFEGHRSVGVGRSRAIVPSALERNGDF
                     TRSFAQGGQPLTIFDPATTQPSGSGYVRSPFPGNIIPASRLDPVAVAAMKYYPMPNVT
                     PTASNLENFDIPNMNELRWASVSARGDHQIGPAHNLFLRFGWNHRTDPSAPFYGEASR
                     PAGNPTSGQDVFARGNIAAGAGYTWIVSPRIVMDVRAGFTRYFDGEFMFGEGFDIATL
                     GFPASFANSIAFANFPRFQMSGDVQHLGAGRQAQRNVVNQYNPLVNFHTTMNRHALKY
                     GFRYQLAQSNSFQPGRSSGRFDFNRVFTRGPDPTRSTTTAGHDVASFLLGAPASGFAD
                     MNASMALQNTYLSFYVQDDWKATNRLTLNLGLRFEHEGPVTERFNQGSAGFDFDVASP
                     IESAAAANYAQSPIPELASLSVKGGLGFLNTGGVPRGNLKTSALMYAPRFGFAYRVTD
                     WIVWRGGYGMFYVPNNVSNFRADGFSLATRMITSLDNNLTPFHLLSDPFPNGLSMPPG
                     NANGLMTGVGQSLTAGRAPAGTVPRYLNGMSQQFSMGFQFVLPAQVSVDASYSGNVSQ
                     RLAMTRGLNQYPNEYLSLRTRLNASVPNPFYGVVTDSTSSLSQRTTTVSQLLRPFPHF
                     TGLTEAVLPYGRSHYDSMQLQVTRRMAQGLYFGAIYTVSKLMEATSYLNSNDAKPERV
                     ISNSDRPQRFVVHGIYEMPFGKGRRFLSGAHPVVGHVLGGWQLNWVGTFQSGAALSVA
                     GERLFRSEDNPRTVDEWFDVTQFVPQEPFTLRQLSSRIVDLRAAGTRKWDFTLAKSFP
                     IKERVQFRFSAEFYNAFNTTHLGSPNTTVTNASFGRITSTTEGPREIQLAGRLSW"
     gene            complement(24085..25395)
                     /locus_tag="VFQ79_11375"
     CDS             complement(24085..25395)
                     /locus_tag="VFQ79_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0140309.1"
                     /translation="MTVVSPPTTLRRVLRLRHLVFYGIVLIQPTAPMPLFGVASQVSK
                     GHAVLAVLIALVAMLFTAIAYGRMARVYPSAGSAYTYVGREIHSLAGEATGWAMMLDY
                     ILNPILCTIWCSKAAGNFLPAIPYGVWVVFFALLFTSLNLRGVEASARTNTFLACGLG
                     GVIVIFFGAAARYLWAAPPEAGAWLLPFYNPATFSSSDVLTGASIAALTYIGFDGIST
                     LSEEVENPKRNILRATVLTCAITGVLATAEVYIAQVIWPDYTTFPDADTAYVYVGGRV
                     GGPGLFALLNFSLLVASVGSGMGAQLGAARLMFAMGRENTLPRKFFATLSRAAVPANA
                     VLLVGALCVIGGFALSYQLAAELLNFGAFIAFMGVNVAALRHALRSGNRSMAFVLPPV
                     AGFLVCFYIWLNLRPVAQVLGAGWLTLGLLYAMWRRRNGSYAEA"
     gene            complement(25392..26711)
                     /locus_tag="VFQ79_11380"
     CDS             complement(25392..26711)
                     /locus_tag="VFQ79_11380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0140310.1"
                     /translation="MLPKVEPKLAIDGGSPVRTEPLPLEFPGVHHIGEQEIEAAVRVL
                     RSRSLFRYYGVDLQCEADRFEADFRAFTGARHALAVSSGTGALHVALAALGAGPGQEI
                     IVPAFLWVSVAAAVVNLGAVPVLADIDDTFCLDPADVERRITPHTRGIVMIHMSGAPG
                     DVRSILDIARRHGLFLLEDCAQCAGGTIGGRNVGTFGDAAVFSFQMNKNITAGEGGCA
                     ITDDDRLYARMFASHDLGYMRDETGRLVTSDPDLLLWGRGYRMDEIRAAVLRVQLAKL
                     PRIVGAMRHSKRRIRAALQGMPEVGLRRLVDSAGDTSCFLLTIFRDGATAAAACDALR
                     AEGIVTYPQGVANILLKDFGLHAYYNIASLVRRGSVDRARFPWSHPSNQGLGGRYERG
                     TCPVADDLFERTLMLPIPSCLTQGDEDDIIAAYDKVTTALAERRETS"
     gene            26866..29223
                     /locus_tag="VFQ79_11385"
     CDS             26866..29223
                     /locus_tag="VFQ79_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEU0140311.1"
                     /translation="MKTPLLIACTATALMVASATESPKYLDPKQPVSVRVEDLLARMT
                     LEEKIGQMNMPCVYLPQLGGGRSSGNSRVLDDRIVAARMEGTKRFAAGEFAEGVGPGG
                     GFFTLANTVLYEGPRQQAEFFNELQRIALRSRLKIPLLQTEEGTHGFMASGATIFPEG
                     PALGSTWNLDLIERIYAAVAREARSVGVHQLYTLVIEPTRDPRLGRNEEGYSEDPYLV
                     SQIAGRIVAGIQRDDISAPDAAIAGLCHFPGQSQPVGGLERGPMEMSERVLREIFLPP
                     WEAGIGKAGALGVMLTYPAIDGVPSHASSKLVKGILRGELGFRGLTLGEGGGISTLVE
                     EHVAADAKEAGEIALRSGLDVGISLERGYLKPLADGVREGRVPLALVDGAVRRILTMK
                     FELGLFENSLVDVDHAMKTVHTPEHRRLALEAAREGIVLLKNDKNMLPLRRDLKSIAV
                     IGPNADNIRNQLGDYIARKVTTPVTTVLGGVRAIAPNARVQYAKGCSVLGEDRSGFAE
                     AVRIAKESNVAIVVVGENERHSPEGGTNGEDLDVASLDLTGVQEDLVKAVAATGTPTV
                     VVLVNGRPLSTRWIAANIPAVIEAWLPGEAGGTAVAEVLFGIYNPSGKLAVTVPRHVG
                     QLPIYYNSSPSRLPAERGRGPAWRGYVDLEGSPLYPFGHGLSYTTFEYSNLRIEPKSM
                     PPGGTVRISVDVENSGKLAGDEVVQLYLRDNVSSIVRPIKELKGFRKIHLAPGERRTV
                     TFDVTPEELAFYNRHLERVVEPGTFTVMIGRSSEDIPLSGLFEVR"
     gene            complement(29159..31138)
                     /locus_tag="VFQ79_11390"
     CDS             complement(29159..31138)
                     /locus_tag="VFQ79_11390"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0140312.1"
                     /translation="MFPSTRLVTHPSGGGNDSRIGKGRPTGSTASRPALLLGPERDLF
                     MEEFEKDLRFLQVFMLMMNRSCPIRNVDLVWTENGPLGRSGWRHMGINEALGGPCSHL
                     LHCSPGNPHPPFCNIVNDLGRREAQSCLVSDKAAEERVSRTGKAEVYRCHFGLLDIAV
                     PVLSDGAHIATLFSGQVLNSPPTEEGFAQIVKDVEHLSYINLSELREAYFRSAVVSDE
                     DIQSTVGILEVFAEYLASSWERLCKAVQQERQHYRELEIRRKEFAYLVLTGASTETAD
                     ARAALARTGLRNCPNRVLVARWPMSEGTLRVDSDREFTAILHAIEEVCERLDNVVHAF
                     LQGRGVCMFLYERPGSRKQVGRLNAQELAQSILDAVAQRSSVHVRVGIGGSTNEFARL
                     GESYREACVALSTTTAPIAVYRASSEPECEATVLAAQIPELLKERRFEEARLALHSLP
                     LHVERQFGADLALEKRLLLSAVGAMLSAGQSLGCDPAVLAELRKKAGESLGRASNSFE
                     VQDGYSIAAETILDEVRQIYLDKGSRLVDRVCRLVERSIAEGTTMTQSSLARALGIST
                     GHLSRVFRQTTGDTFERYVMRRRVELARRLLLDPAHNVSTVARQCGFKDASYFARVFR
                     RLVGSSPAEYSRHPVNALQISRSEEYLPTSGQSLQ"
     gene            31405..34662
                     /locus_tag="VFQ79_11395"
     CDS             31405..34662
                     /locus_tag="VFQ79_11395"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140313.1"
                     /translation="MKRGILLCCLAAVSAPLFAQSITGTILGTVFDPTKSVVPSAKVV
                     VTNTAQGWTRETTTDETGSYTVAQLPPGRYQVDVSAPGFRTVSISNIELLVDQRARVD
                     ADLQPGTVTEQITVTAGAPLLETDSNALGQVVNTRNIRNLPLNGRRFFDLALLTAGAA
                     PQGTTFSSVVWGRVTGVSLAGTRDINVSFLVDGAETRDERYGGTFQFSSVESIQEFKV
                     QQNFVDAQYGHASAMVSAVTASGTNEFHGALYHFLRNNKLDARNFFDRGEVPPFRFNQ
                     FGGSLGGPVYLPGYSGKDRSFFFFNYEGQRRRRQTTSIATVATPAQRAGDLSTLPLQI
                     YDPLSGNAATGRRQPFAGNLIPADRIDPIARALMDYWPQPNLPGNAANYITTVGEKID
                     YDQVTTRFDHHLSSKDRLMGRYNYIDQPFFRGNYAPLAGSVAPLRNNGVVAQYTRLVS
                     PRAVNEFRFSYSRSSAQFAQEPVSENLAAQIGLKNTSTDPKEFGLPSVSISGYNGFGS
                     FSPTIMNITDRFQWADDFTYTRGRHNLKAGIDLRRLRYRQRSAQDPRGFMQYQNNFTT
                     PGPGIGGGGDALADYLLGTPGFWQVQLEELGFDGRMIQPGIYFQDDFKVTSRLSLTLG
                     VRWEYNSPWVQPRNNMAVFNFETQQLEYALRDPFAFRTSTEPGDVVRRSIINPQYNNW
                     ADRIGLVYRLFEKTVIRAGHGWYWNNVNNNQLTQSMSLFYPFVYNPQNTESNSQLTPS
                     FFNSNLYPARPTGTGLPYGPTFAFFTVQKDFPRPYTSQWNINIQQSFGENYMLEIGYM
                     GNQSHHMPSYTNYNQARLPDPSIPFAQQPLQSRRPYLNYGSINMFDRMGNASYHGLTA
                     KLEKRFSSGLSFLASYTWSKALDTGTDISSDPIKQPGDAKSYRSMASLDVGHRFAGSY
                     SYELPFGRGKKFLSGAGGFANAVVSGWQVNGITVFSLGVPFGVSAPSSIPDVDARFVT
                     ANRTCDGQLPRGERTRLRYFATECFSLPAPGTFGNSARNLWHGPGINNWDVSFFKNFR
                     LWNERSNLQFRFESFNFFNHTQFGNPSSGLPSGTFGVINGAKAARENQLALRLSF"
     gene            34665..36920
                     /locus_tag="VFQ79_11400"
     CDS             34665..36920
                     /locus_tag="VFQ79_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140314.1"
                     /translation="MSRFCLIAGLLLQVPLLVCGQDTVAAFAEKAYAALAVEEPYTFH
                     KELSEWREPLRRDAAARPGVSEMSIGQTGWRLLVRGDSGLVLRTAAEEFSEYLERSMQ
                     VKIAVERPASLAGWNNERNAIIAGTREQLPGCGESLKGPKDYRIAVSPDRVIVCGYDE
                     RGAMYGLFDIERRMNLREAPFLDRTLDITRHSLFKARMVLSGLGWEEWPDRYLALLAR
                     YGFDSIFASVYVNPNGVPGPPPYWDRMKKQDPKRVHDLIRRAARYGIDLYCPIIYLYT
                     GEPENEAGLRKLVRDIVTEFPQIRGYILLTEGFFYRSFFGAGGQGNIDLRDWVRHWAR
                     GVAIVSEECRKRNPAIEVLPWEYNIDFRPNQVDLKRYGTAQLPVETIPLLTFENGKAF
                     ERDGEQGSLKDYAINEVGPAEVTVAQIDEARKRGMRGVYSKADTFASWQYGTFPYLPF
                     PYQWHARYMALEKYKIDGTMETWSYGFKPSWMAEVRAWYSWSGYPELNRLLTGMARRE
                     FGAGNEDLVLTAWKRFSDAIKLVPDTGPNMGTNNSVATPLFFEKPKARAMTLDHSWED
                     PAIWQRHSQLWPYWPYTPRRLILVPDFTNESNAAERYARPFSLGVFNKYLTLAADQME
                     QGLRSYRQAALKAPASKRAGAMREVLLAEQLQRMIRSDRAVLEFEDMRLRLAKTGDRA
                     MRLDLLKRMTALLEEERTRTQHSLETARRDSRLGYEWEQDYIYTPDHIEEKLKLIDLT
                     LKEQIPAYRNR"
     gene            complement(37029..>39273)
                     /locus_tag="VFQ79_11405"
     CDS             complement(37029..>39273)
                     /locus_tag="VFQ79_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140315.1"
                     /translation="NSLNFLSLASTPVNQGVTNIRIDQRFSDKDSLLGRYTVTRNTRQ
                     GRGWGLGPADPDIFARRDQRDNHNVIVTETHVFSPAVINEFKANFTRQNLPFLHPSFG
                     GDWPDQLGMPAIFPRDLFPRVGISGMLPLGSDPFAAGIRAQHTIQIGDSITIVKGKHQ
                     IKAGVDQRWFRLNYTQLRNRSGNFSFTAGLTGDPLRPAGTGVGMATFLLGEVSGGTQE
                     FVPAFSFHSWSTAMYFQDDYKVTPRLTLNLGLRYDLASEPVERHNRFSNFDPYIMNPE
                     TNMPGVLTYADVTSPRHFVDRDYNNLGPRFGFAYDVTGDGKTAIRGGYGLIYMLVESG
                     DTQGDNSNSFGFASATPFVAPGGGPFRAFKFSEGPSALIVPLGPAGGPSAFRGQNVRY
                     QDRNAPTPYLQQWNLAIQRELPGRWVGTVAYAGNKGTKLFGGNYNLNQLDPIYWQQFG
                     LGLQDQLENPFFGQIATGALSARTVQRSLLLQPFPDYGSITTYAAHNSSSIYHSLQVT
                     IEKRYASGLSALVSYTNSKLINDSFSSAGSSGAIGEYRLGRFNRDLDRAIDQNDVTHR
                     LVGSAVYELPFGPGKPLLGGARGVLGHVAGGWQVNTIVTIESGTPLSVRGANNFTGIN
                     WPDVVRDPTLPSSERSVQRWFDTDAFRNPPNFVLGNAPRTLPTTRGPGMVDVALSAFK
                     NFSIYERANLEFRAEAFNAFNHVNHTNPNVTFSPNAAGVNTNAQFGRITNSLAARRIQ
                     LGLRLTF"
BASE COUNT         7842 a        12005 c        11615 g         7811 t
ORIGIN      
        1 aggagattca aggcgatgct gaacagcaca actaacccga ttatgattga cgttcgatac
       61 atgatgatcg ttcctgttgt cgactaactc atcaatttgt ccgatataac gccgctcgcc
      121 gctcatggct ggagaacagg agaagccgat ctccagtcgc tccgattgcc gacacaacag
      181 taaacgcttc gccgtgcgac tgaggtggaa acttgcagcg gtacctatcg ctaagcttcc
      241 cctctccgtc gaaacggagg attaccgagc cggaacccgg agtgtacccg gacaccaccg
      301 cgaccaggcc gccccggccg tcggacacgg tactgaatat gcggttttcg aaaacatttg
      361 gaggcagctt aggcgcggat cgccacggga gttctggcgc aaccagttgc ctgtccgtgt
      421 aaatgttgcc gcttaggcca agcaactgca ttcgaggagt ctcgcctgtc tctataacgg
      481 caacccgacc gctggacaaa ccgactatct cactttggtg agaggcgccc gatcgaagtc
      541 gtatgatcgg ttgctcgtca ggttccgaac gcaacacgga gttttttacg tcgagcccta
      601 tcaatcgatc agagtaggtc aaaccgctca ccgtatgatt caaaaccgca atattggaaa
      661 tcctcccaga ctcattaatg aaaaccagac ggcaacgctc gcgagaacgc tgcagaacaa
      721 ccggcagttg accggcgcca agacagaggc gagtcgagat ataaccccca tccgttggaa
      781 ggacagttgt ccgcagagtg gttcccgtgt gactcacttc caccaatgcg ttaaaggatg
      841 gcagtttctc caaatagaag atactcccat cttcaactac atagttatcg atgtgagagg
      901 cactggaagt tggaatggca aaggcgccaa gcggctgtaa aacttggaca gccgtaccgt
      961 cttcggccaa cgccgaaact tggctgaggc acagcgccgt aacaaacaac atcagcagca
     1021 caacgagaaa gatgcctgca cgccaagagt gtgacatcga aatcgtcctc cttcttcaag
     1081 cttgaccgct tctacacagc gtatcgattg tgagctttcg tctggattat atcaccaaga
     1141 gttgcgacga tagcttacct gctttgtctt gaatctctat gcgaattgaa cctgatcgct
     1201 gcctggctgc ggcgcagccc ggcagcataa acattcgtgc cgtaaatgta acatctctct
     1261 cgctgttggt ccgcttataa ttgagcttcc ctgtgagcag cgccaagctc caacaatacg
     1321 tcgccatgat cgaacacgag ctcgcctggg ctcggctgaa aatccaggca ccgcagcaag
     1381 agctacggct ggagcgcaac aagaaatacg gctcgtcaag cgagactctg agttcgtggc
     1441 aagcggaatt gcttgacttc aggctgagtg tgagcggtga agcggcgcag tcgaagtcgg
     1501 gcgcgaagcc ctggccatca agcttgctcg cgaatgcaaa ccgcatcccg ggcggtagac
     1561 tctgacgaaa aatctgccac atgtcacaga cgaggtcgcg tatgtcgatg gcgtctgcaa
     1621 tcattgcggt gccgaagccg cactcatcgg tcagactcgg atccggtgag ctgcccgaat
     1681 tcgcggatca acggcatcgg tcctcgcaac tccggttccc ctggtgcaag ctggcgtgcg
     1741 cgcgccatca gtgtagagct gtattggaat accgtagggt ctcctcccgg ggcgcgtctc
     1801 gcccgcgagc gcataaacag gttcggcagg gctgttgccg ctgccgccag caccagcgga
     1861 ttcgttgtac gttccagctc accccgcgcc tgagcgccca accgctccgc ttcactcgga
     1921 cgccggcccc tggttccaaa gggaccattc atgcccaata tgtccatcgc atagaagaag
     1981 ccaaggtgag acgccaacac gcgattggac ggctcctgtt caacgagccg ccgcaaaagt
     2041 tcctcggcgt catcaggatg ttcgacataa aggaagcgag cggcgttgac ctgtacgctc
     2101 gcatcggttc gattggcgtc cgccgcacgc aaccaggcgt tgcgcgcgag ttcatgatcc
     2161 gcggcatcgg catagggtcc ccctgatgca tagacatatg ccagcgcact ggccgctacg
     2221 tcgctggccg gactatgctc gatcagatac aggatgtgcc tgagccgcac ggcacggaag
     2281 accgggttca tgtaatccct gtagtgcgtc aacaaccggc ttcgcgtggc gatatcacca
     2341 ggctccgact gaacgcgggt ttccaactcc gctacctctg ggggcgagag cggaggctgc
     2401 gaggagagcc gggcgatggc ctgtgcctcc atcgcctgtg ccgatgcaat caagaggaca
     2461 gcgcaaagaa gaggccgaaa atcggatcgc ataactcgtg ctccaggagc attattccac
     2521 ttcccgcgtg ccctgtcccg cacgttgctg gtggaacgtc gccctgggat cgcgcgccga
     2581 ggcatttccc gcgacttcta cagcgaagcg catgagcagc gaaccaccgg cgccgagaag
     2641 tcccgcaacc attcgcttct ttctgttctg gcgcggcagc atcaacgtca cgaggctggc
     2701 tgccgtcaga acgcttgccg tcttccacat cacactgctc agccctctct tcaaaggacg
     2761 tgccaccgtg ggcacgcggg cagcttcgcg ctccatcgaa tgggccgcgg ccaactcggc
     2821 caggcgtccg ccaataccga agtatcgcgt gatgcggcag gcgcgctcgt cttcaaacat
     2881 cagatcgaag atggcaccgg cgctcgccgc cgccgacgcc ccgaacaaaa caggaagaat
     2941 acgtcgcgac tccttccaga tggggatgac cgtgttaccc gccagcaccc cggtataggt
     3001 tgccagcgcc gctccgaacg ctcccgaaga atagcccgcc gcttcgccga tccgttgcag
     3061 gattccatgg gagtgccttg caaatagacc cgccgtgata gcggccgaag gcgccgctgc
     3121 cagaatccac gcgcccatat tcatcggcga agtcgggcgg aacacgcgca gcatatacag
     3181 gaaccgttcc ggccgtccga ggtccgcaat cagcaggccg ccgctgatgg tagagccgat
     3241 gatcccgatc caatggcatc gccggatgaa cctgtcgagc tcctgcgagc cgtcaagctg
     3301 agccgccgcg cccagaacca gcgaggcgcc cgcggcacca ccagtgaaat agtaaagtgg
     3361 aataaagggc ttccaaaccg attgcttcag gagcggcctg tcgtagtaag tggggtcgcc
     3421 cgcacgaggc accggcaatt gctcccaggc gacacctgtg ggtgcaaggt gcgcctcgga
     3481 catgctcgta acctgctggg ccccctcgcc cgtcagttcc gccagtgtgg tattgatgtc
     3541 gcgcccgtcc gtctgaaccc gatccaccgg cctgccgtcg aacgtctgct tcaagagcgg
     3601 cctccgagtt tgctgaagaa aaccgagccc accgccatca gcgcgattcc ggcggcggcc
     3661 gctgccatgg atgcccagct ccgccccacc tctttggtcg gaatcacggg gtcgggaggc
     3721 agattgtaaa cctcgggttt gtccaccagc agaaagaacg cgttcaatcc ttcggttccc
     3781 ggctgcgacg ccgcatcttc gccgtacagg taagcttcgc gcattcccat atcctgtagc
     3841 ttttcgacgc gcgcacgggc acgcgttcgc aactcgtcga ggtcaccgaa ctgaatggat
     3901 tccgtcggac acgccttcgc gcaagcgggc gtcatgtctt cgcgcaggcg gtcataacac
     3961 aacgtgcatt tccatgcgcg tccgtcttcg ttccgccggt cgataacgcc gaaggggcag
     4021 ttcacgatgc agtatccgca gccgttgcag atgtccggct gtacgtagac gctatcgaat
     4081 tccgtgcgga tgatggctcc ggtaggacag ctttcaaggc accccgcgcg cgcgcagtgc
     4141 ttgcatacgt cagaggaaaa cagccatgaa aaggcgcctg catcctgcgt ggaaagcggc
     4201 accggccttt cgatgaacgc gacgtgacgc caggtggaag cgcccaggtg gatcgtgttg
     4261 tcatacgaca tgcccgtgaa caacaggccg tcgtcgggca actggttcca ctgtttgcat
     4321 gctacctcac acgctttgca cccgatgcac aaagtggtgt cagtgaaaaa gccctttgcg
     4381 cctgccaccc tcagtgcccc tctttgctct cttcggcttt gaggccgtgc tgcgactccg
     4441 gcttgcggcg cgcgggcggc agatccctct gccagcgctt tgggtcgatc tcgcgcaccg
     4501 gtggacccga ggtcgccgca ctgcgctttc tgcttttccg gccaggctcg atgtcgcccg
     4561 tgaaggcttt cgattcctgg atcgatacat tcggatcggc cacgaatccg atcaggtcgt
     4621 tagcggcgtc tccccgcgca aggcccttgg tggaccaatg atagggcaat ccgatctgat
     4681 gaaccagcct tcctttgatg cgcaagggcc gcatacggct cgtcaccagc actcgcgcct
     4741 caatttccgc gcgcatggtt cgaatggtgg cccatcctcc gttcttcaat ccgcgttcgg
     4801 ctgccagctc cggcgagact tcgcagaaca tttccggctg caattcggaa agccatgaaa
     4861 gccagcggct catggcacca gccgtatggt gttcggtcaa gcgataagtt gtgatcacgt
     4921 atggaaagcg cggatcgtcg taagcccggt ggtagggatt atctttccgc caccactcca
     4981 tgcgctcggg attgcattgc tggccatata gcggattctt cactacggat tcctgcggtt
     5041 cgtaatgaac cggaagcggg ccgtcctgga ggcccgtcgc agcatacaac cagcctttgc
     5101 catcggcctg catcacgaaa ggatcggttc cgccgatcgt atctttcccg cgcgcgtctt
     5161 tcgaaggatg aaaccagggt gggcgcttga cgatgaaatc cggcgtgtcg tgtcccaccc
     5221 attcgccctt ttcctggtcc caccacacga gcttcttacg ctcgctccag ggcttgccct
     5281 ctgggtcggc cgaggcgcga ttatagagga gccgccggtt gtgcggccag gcccaggccc
     5341 attccggcgc aacccaattc tgctcccagt gcggcttacg gcgcgccgtc atgttttgac
     5401 cgtcgcgata gcacccggaa taaatccagc agccgcaagc agtggagccg tcgtccttca
     5461 aagaggtgaa tccctcgacc ggctttccat ccgctaccgt gtatccgttg atctcgtgta
     5521 gtacggcttc ggctttcggc tcctgcttcg gtccttccaa cgggtagtcc caggtgacgt
     5581 cgcgaatggg acggttgcgg ggctgatcgt attcgcctgc atacagcttt ttcaagcgca
     5641 gtcccagctt gtagatgaaa tcgagctcgc tcgcggcgtc accgggcggc tcgacagcct
     5701 tgtaatgcca ttgcagcagg cgttgcgtgt tcgtgaaggc gccgtctttc tccacgtgag
     5761 cagcggcagg aaagaaaaaa acttctgtcc cgatgtcttc ggtacgaatc tctccgcgct
     5821 caatctccgg tgcgtcacgc cagaaatccg ccgtttccgt tacggagaaa tcgcgaacga
     5881 caagccattc gagccgccgc agccccttac gatgcagcgg cccgttcatg gacccgacca
     5941 ccgggttttc gcccatcacg aaatagccct ttatgccgcc gtcggccatg ttgacgacgg
     6001 tgttcatgtg cgaatggtcc gctgtcaacg ccggcaggta gtcgtagcac cagtcgttgt
     6061 ctcttgtagc cgcctcgccg aaccacgctt tcagcagcga gacgaaatat ttcggaaatt
     6121 caccccacca gccggaaggc gaagtattga gcgccagata agtggtgagg tctttatcgt
     6181 gcttcgcctt gggcatcggc aggtagccgg gcagcagatt gtatagcgta gggatgtcgg
     6241 tagagccctg tatggaggcg tggccgcgta aggccaggat gccgccgccc ggcctgccga
     6301 tgttgccgag cagcagttgt acgatcgccg cggtgcggat gtattgcacg cccatcgaat
     6361 gctgcgtcca accgacggca taacaaaacg ccgatgtcct ctcgcgcccg gaattccggc
     6421 acaatgtctc cgccaccttc aggaacaggc tcttcggaat tccgcaggtc tgctccacca
     6481 tctccggcgt ataccgcgaa aaatgccgct tcaggatctg gaacacgcac cgcggatgct
     6541 gaagtgttgg atcggtgtgt tcggcctgga tcggattgcg ttggatagtc tgatcttctt
     6601 cgtccgattc tcttgctccg gcggccggtt cggggtcgac gccttcgtac tgccaggtct
     6661 tctcgtgata ctgtcccgtc tcttcatccc acccgccgaa gagcccgtcc agatcttcgg
     6721 tgtcccgaaa ttccgcggaa acaatcgccg gtgcgttcgt gtagtggaca acgtaatcgc
     6781 gaaagtaacg ctcgttctcg ataatgtagc gaacgatgcc gccgagaaac gcgatgtcgc
     6841 tgcccgcacg aatggggaca tgtatgttgg caacggcgct ggtgcgtgtg aaccgcgggt
     6901 cgacatggat aatcgtagcg ccacgctcgc gcgcttccat cacccagcgg aagccgacgg
     6961 gatgacactc ggccatattg gacccctcga tgacgatgca gtccgcgttc tgcagatcct
     7021 gctgaaacgt cgtagcccct ccccttccaa acgatgtccc caaactgggg accgtggagg
     7081 agtgtcaaat acgggcctga ttttcgatct ggacgattcc cagcgcggta aacagcttct
     7141 tgatcaggta attctcttcg ttgtccagcg tggccccgcc aagatgtgcg atgcccatgg
     7201 tgcggcggac cggatggccc tcctcatccg tgtcctccca ggtttcgtca cgggtgcgtt
     7261 tcacccgctc cgccaccatg tcgatcgctt gctccagcgg tatcgtctcc cattccttgc
     7321 catgcggacg ccggtagaga acatgctgca cacggtgaga accggtaacc agttggaagg
     7381 ttgccgcgcc tttcgggcac aggcagccgt gcgatatcgg agaggccgga tcgccctcga
     7441 tatcgatgat cttttcgttc ttcacgtaga cgagttgccc gcatccaacc gcgcagtaag
     7501 ggcaaatcga agatacgatg cggtccgcgc tcgtcgtgcg cgctgtgagc gcctccgtac
     7561 gcggcgacgc cgcagtccgc tccaaaccga acaggtcgcg ctgtttcagt tgccggaaca
     7621 ggggccaatt ggaaagagaa aagccgtatt caccgggcat agcgaacgta ggacgcgcaa
     7681 agttcccgct ttgttacggc ttagcgcgaa aacttctgct cagacacgat gccctcgccc
     7741 tctttgatgg tcaccatgcg accggcagcc acgtttttga acgattcggc acccccgttg
     7801 ggccagcgta tttcgagatc cgcactggtt gccttaccca ggccaaagtg cagacggagg
     7861 tcgtttaccg aatagaagct cgattggctc aggaccgcct gcacctgcat tcggccgcca
     7921 tagcgcgcta tcactttcgc gccgatggcg gtgcgattcg acttcgtccc gatgagcttc
     7981 accttcagcc actgattgtc accggagatg tcattccgca acagcgatgg aggttcgtta
     8041 aggttcacga tcagcacatc gaggtcgcca tcgttgtcga aatcgccaaa ggcgcagccg
     8101 cggctcgggt gggcggcggc gacgcctggc cctgactctt cgatgaactg ctcgaactgt
     8161 ccattgccga gattgcggaa aaccacgcgc ggggtcttat agggatatcc gggcaccttc
     8221 ttctccactt ccggatagac gctgcctgta acgtggaaca ggtcgggcca gccgtcgttg
     8281 tcgaggtcga cgactccagc accccatccg gtaaaccggg tctcgacacc cagcccagcg
     8341 cgaatggtca tatcgtcgaa catcgctttg ccatcgttgc ggtagagaat gctggtgtcg
     8401 tcggagaagt gcgttttgta gatgtcgagc gcgccatcca ggttatagtc gccgataccc
     8461 aggcccatgc ctgcctgctc catgccgtct tcattcaacg ctactccgct ttcgaggcct
     8521 atttccgcaa atgtgccgtc atgctggttg cgcagcagaa agctgggcgt agagtcgcac
     8581 gcgacgtaga tgtccggcca gccgtcgtta tcgaaatcgg ccgctacggc cgtcatcgca
     8641 tagctgcctt tcacactgcc cacacccgat tcgcgggtga cgtccgtgaa ggtaccgtcg
     8701 ccgttgttcc ggtataatgc cagcgcgccc ggcggcaacc cgcgcggtcc gcagttcacc
     8761 ggaacgcctt tccatgtgca gttggcgttt tcgcccggct ttggaacgct cttgaaatcg
     8821 aattcgagat agctgcccat aaaaagatcg agcagcccgt cgcgattgta atccacgaac
     8881 gtgcacccgg aactccatcg cggattcgcc tgaagcaggc cggccttctc cgtcacgtcc
     8941 gtgaacgtgc tgtcgccatt gttgcggtac agcacattct ggccgaagta ggtgacgaac
     9001 aggtcgtcat ggccgtcgtt atcgaaatcg ccgatgcata ccgccgatgc ccagcctgtc
     9061 cgcttcagcc ccgctttgtc cgtcacgtcc gtaaaggtgc cgtcgcgatt attcttgtag
     9121 agccggttcc ccgcgtgcgg aggcgcccct tccaggcgcg tcccgccgag catgaatata
     9181 tccagccagc cgtcgttgtc atagtcgaga aacgcgcacc cgcatccgac cgcttccaga
     9241 atatatggct tctgctccac accgccgtag atggtagggg ccgtgagacc cgcctgtttc
     9301 gccacgtcgg tgaagcgcgc atgaaacggc aaacccgacg gcttgccccg gcgggtgggc
     9361 tttacgttgc gtgaggcaac gccctggccg aaagcgttca gagcaggtat tgtgagcagg
     9421 ccgagtaacg tgcggcggtt cataacgtac cggtgatctc gaaggcgatg gcgtgctctc
     9481 ccatcgccgt atcgggcagc cgtacaatga tgccctgcgc cgccgccgac cattcaaccg
     9541 gcgcaggcgt cccaagcaac tccacgcgcg tgccgccgcc cgcgcgcacc gcgggaatgg
     9601 cgatttgcgt aggaggccgt tttccgtcct caagcagata gaacgcatat acggtgttgc
     9661 cgcggcgcgt gtatgcaatc ctcccctccc tatacggagc aaaagcacgc gtgccgtgaa
     9721 tcgccttgcc gttcaccgcc atccatcgtc cgatctcttc gagacgcgcg accgcttctt
     9781 cgggcaattg tccatcgggc tgcgggccga tgttgagcag cagattgcct cccttcgcaa
     9841 ccacatcgat caacaggtgc acgagttcgc gtgtcggctt gtacgtgtcc cccggtttgt
     9901 aggaccattg tgtacccatc gtgatgcaac tctcccacgg gccatcgagc ggtttctcag
     9961 gtaccttctg ttcgggtgtg cggtagttct catgcttgcc gcccacggtg cggtccacca
    10021 cgatcaggcc gggttgatgg cgtcgcgcca ttgcggccag acggtccata tcgatattct
    10081 gcttcggcgg gcgcacctgc ccgccatcga gccacaggat atcgatatgg ccgtaaccgg
    10141 taaccagttc cccgatctgt ccgtgcacga actggacaaa ctgcgcccag cgccgcgaat
    10201 cggtcagcgt gtcgtagttg gggttgcggt cttcggcgaa gacatccggg cgccaatagt
    10261 aggggctgtg ccagtcggat ttcgagaaat acgcgccgat gccgagaccc tgcttgcgaa
    10321 aggcgtcaaa cacctcacga acgatgttgg cgcggggatt gcggctgaac ggtaccgccg
    10381 gcgcagtgac acgatattcg gactggcgcg tgtcgaacat cgcgaaacca tcatgatgct
    10441 tcgtcgtgaa caccacatat cgcatgccgg cggccttcgc gtggcgggcc cacacggcgg
    10501 gatcgaattc ggttggattg aatgtgcgcg gcagtgcgaa atagcgtttg cggaagtccg
    10561 ccatctcttc gcgagtggtg atctttgggt tggaccactt gcgatccgcc cataccagcg
    10621 gccaggattc gataacgccc atctggctgt atgcacccca atgcagcatc agcccgaatt
    10681 tccgatcctg gaaccattcg agccgttgct ggaggcgatc gtccgcggcg ggtaaagatg
    10741 cggcaagaag cagcagggcg gcaacgcgtc tcacttcacc agcctccagc cttgcgccgt
    10801 aacttccaat ccggtgtgac cggcgtagcg atcgtatacg cgctccatct cctcgacgga
    10861 atcgaaataa cccacgagga acgcagcgct gaaatggtca cccggcttta ccgggcggcc
    10921 gccaaattcc tggatcatgc atacgtatcc gcgttgatgg caccatgctt cggatacgac
    10981 agaggggtcg agcgtcatgc ccgcgagcca cggtcccgcc gcgccggttt tcggatcgcg
    11041 cagacggtaa gcgcggatga atcgccgtgg agtcttccct tccccgcgcc ggtacaggaa
    11101 cttttcgtcg ggcgcgaaat cgtcgagaaa ctccttcgcg gggatccggc cgaggtagct
    11161 gaggaatatt tcgctgaacg tatcggtgcc tttatgtttg atgtgacccg gcatatcgat
    11221 gcgcaaaaac agcgcggggc tttcattcac cgtggtgatg cggtcggacg agacgaaata
    11281 acgttgtcct tccggaaaga cgatggtctg ctcccacaac gatcccgttt tgcgccccgg
    11341 cgcggcgatg cggtagcggt aatccatctt tacagcccgc aaaatccttt ccacgaatca
    11401 cgtgcggatc cagcttccgg gcttgtgtac agatctgagg cccctcgata ctacgcttgg
    11461 cgatcctgcc atggaaagcg ttgttgaaat cgtaccggag gtcgcctgta aggcggtcgc
    11521 ggtatgcctc gtcgctcccg ggctccatca gccagtcgac gatatcgagc ccgaagcccg
    11581 cgtcgcggaa acccgtcttt ctgtcgagaa acgacccgcc tgcaacaccg gaaacatagc
    11641 cctgcttgcg cacaacagct tcgacggcgg ttccctggat gctgatctcg tccacagttt
    11701 ccgacacctg gaaagccgta aagaaaagca gaaatgccaa gctcattgac gcgcagtata
    11761 tcagttgcga agggcggtct tcgttatggg ttccccggag tttcgcgcca catccgttgc
    11821 agatcgtaca ggttgatcag gtgctgctgt atgatgcgct cacctccgcc cacttcgatc
    11881 gtggtggaca cgtcagcgcc gtagccgccg tgtgcggccg agcgcaaatc ggggatgtat
    11941 ccggcccagg ttccaccgac tgtgtaggtg tagccgtaga acagaggatg cgccacactg
    12001 gcctgggcct tccaggtctt ctgtagcacg tgaaatggct cgccgggtag cgtcgcgatc
    12061 gcgatgtctt tcccgataag taccgtcgtc actcctactg tcagcttcct gtttttgtcc
    12121 caacggttcc ggaattcgag cagttcgctg cgcgcgcgga tcgggccggc ttcgacgccg
    12181 attacatcct tgctcgcctt caggacttcg gccgcgagga tgtctcccat cgtctccgca
    12241 atggcgaagt ccgcggcctc gtctcccgac cggcccatca acagtggatt gatatcgccg
    12301 gcgccccctt gcacgaacat cacctgcgtg cccggcagtt cgctttctac cttcgtctgc
    12361 atcattcccg ggaagtctgc cgagaacttg cagctcgtct tcccgagcac cacggcatgc
    12421 accgcgtagt gcaccagcaa cgcgcgcgga cgccctgccg tgtcttccac gcggagcagg
    12481 tcaacgccgg ggtccacagg cccccaggga atgcgctcgg gattccggta tagcacacgc
    12541 gaacggccgt cattctgcat ctggagcctg ttgtacccga gttgaattga gccgcgcccc
    12601 acgccaagac gcgccggaaa catcgacgtc gaagcctgct cgatggctcc aaagatcttt
    12661 tgctccaatt cagtccgata ccgcgcgccg gcgtcatcgc gaaccggcgc gcttccaaac
    12721 ggcagaaatg cgggcgccga gtgcgaatgc gacgccgcca ggagcagcac cgtgatcccg
    12781 agcttcgccc tgacctcgtc cttcagccgc tcggatacaa tgctgcccag atcgagcgtg
    12841 acgatcgcca tccgcgagcc gccactctcg aggaccagcg ctttggcata gagcggatcg
    12901 tgcgtaccgt tcgagggtcc gcactcgcga tccctatagc cgtacatctg catagagacg
    12961 gcgggcgtga tgtcaacccg agccactcct gcccgcaact cgggcaccgt ctccgcgtga
    13021 gcagaggcga gcaggcaaag aaagaaaacg atgcgtttca tgcgaaatct cctgtttgta
    13081 cggcccgtca cgacagcggc ggaatcgtat acggctcacg gtagtcccgg ctgaacaacg
    13141 cgttcgcctc gccggcgccg atgaatttcc cggtagcgcg cgaaagccgg agcgtccgtc
    13201 cggttcgcag gctaatattg gccagatggc aaaacgcggc ggcacgggtg ccgatcgcga
    13261 tatcggcagt gagcttccgg cggtcatgcg cgcggacagc atcgagaaag tttttcatgt
    13321 gcggtttggt cgcctcttcg tctccctcca cggctttttc gtccaacact ttttcgtact
    13381 tctccgcccg ccggatttcc cgggacacgg cctgctccgg gctgaggcgg gccagcgagc
    13441 tccggtaaac ctgaacgcct tccgggtcca ccaccatgta gccgagctcg ccgaagaaga
    13501 ggtttccaat gaagctgcgc gtccgcgcgg gaaccaggcc ctccggtgga gtgggcaaat
    13561 tgcgcacatc gaaggtgacc tgcgcatcgc cgaaatcgaa gacggcatgt tgtgtgttgg
    13621 gcgtctcctg gtcgtccttc catacatact tgccgcccgt cgaagtcacc gaatccggcc
    13681 atccgccacg gccaatgccc cacaggcaga tgtccatttc atggacccct tgattgccga
    13741 tatcgccgtt gcccgtatcc cagaaccagt gccagttgta agcaaactta ttcaagctgt
    13801 acggcttgac ctgcgcgggg ccgaggaacc ggtcccagtc gagccctgcc ggcaccgcgg
    13861 catccggcgt atggccaatc gaaaaccggc ggcgaaagca gagcccgcgg gcgtgataaa
    13921 cctgtcctat tgcgccatcg cgcagcagtt gcatggcgcg tttcttgtgg gttatggagc
    13981 ggccttgcga tcccacctga accacccggt cgtatttccg cgcggccaac accatttgtt
    14041 ccgcttcgaa gagattgtgg caggccggct tctcgacata cacgtccttg cccgcctggc
    14101 acgcccatat ggtcgcgagc gcatgccaat gattcggaag cggcagcgac accgcgtcga
    14161 tctctttgga atcgaacagt gcgcgcatgt ccgaatactc ggtggcgttg tagtttcgga
    14221 gcttgcgtac aagcgcagcg gcgcgttcac gcgcagcctg gtggacatcg cagatcgccg
    14281 tgatccggca atcggactcg aacgtcgaat agtagttcag gtgatcggtt ccccggccac
    14341 cgagacccac gacgcccagc gaaacgcgat cattagcccc gatgatagga aattgggcac
    14401 tcgcgatcgc ggcacttacg cacttcacaa actgccgtct ttgctgttcc atctgaatta
    14461 ctcccctggg taagtcataa cttatgaatg ccggctccca ggcctcacag cctctccgga
    14521 atcaatcccg gcgtgtcgct acgttgccgc tcgctctcgg ggttgaaggg gttcagcatt
    14581 tccttaaagc gcccggggcc gggtaagtag ataaacagct tgccgccccg gtcgctttct
    14641 ccgcagaaga ccgcgccgtc cggagcgacc gctatcgcgt caaattgata cgcccggtgc
    14701 gaatagtaag ggctccggtc gacggcgaat ggtcccaact ccgaaaagcc cttcttgccg
    14761 ctcaaatccc acgtatgcag tttgcaggat cgctcaaact caccggtgat gaggtagagg
    14821 ttgttgtctt taccggcggc catcgcacgc agccgccgca taatacgggg cttcccgagc
    14881 actgccacat catcctttcc gatatccagc cgcaccaggt gtccatcgtt ggtgccggca
    14941 tacacgttgc cgctggagtc cgtcgtgaac gcttcgacaa cgggatagtc gtaagaactc
    15001 agtccttccc agtattcgcc tggaagccgt atgaacgtct gctcgatctt cccgctgccg
    15061 ggacggtagc ggacgatgat gccgttgtcc ccggacgtgt acacgtcacc ggatttcgga
    15121 tcgcagtaca aagcccgcgg cacgcttcgc caatgacgct ccggtccgct ataaaccttg
    15181 tgcgcgagca gctcgcccag atccttcgtg gtgcgtgtag ctaggtcgaa aatgaagaaa
    15241 tgcgcgtcgg ggtacgtaat cccataaatc tttgatttat ccgcactcat cgtcatcgca
    15301 taaaccgtgt tgtgcgaaac cggaacgccg aggtcttcta ctttcgccgg cgagtcgttt
    15361 gtgtagcgtt cgacatcgcc agttttggga tcatagcgat agatgtggcc accggcatag
    15421 tcccggtaag gcaaagtaat atctttccag agttgcttct cgatgccctc gacctccacc
    15481 ggaaattcct ttgtaagttc tacgggcgag tacatactaa gtccggtacc tatgtagatt
    15541 tctccgtctt tgccttccac taagccgtga taaacgccgc gcgcatccgc gattttcccc
    15601 agcggccgta ccttgttaat gaagcggttg tagaagaaca ggtacgactg cgtccgtccg
    15661 ctggttgctc cgtagatgaa accgtttgaa tgcgccagga gtgccgaaat gcggctgttg
    15721 tcggctggaa tgagattctg cgcgggatat cccaagtccc ggtagtccat gcgatgagtg
    15781 tcgaaatggg ggttgaccca gcgctgtccc cacaaagcgc cgctcgcgcc gcccagaaac
    15841 agaaatgcca acacgggagc tgcggtccag ggtctcttca tcttcgttct cctatttacc
    15901 ggacggggaa tagcagccga ggcccatgct gtaggcgcgt ctgccgcggc ctgcccggta
    15961 gcgcgggtcg ggctctttaa acgcgcccac gaaccacagc cgtccgtcct tatcgatctg
    16021 cgcaccgccc atacccgagg cggtacgtcc gtcggcggtg cgcagcactc ccacgtcctc
    16081 gcgccggcca gacttcagat cgtaagtcac gaggaacgac gcgcccatta catctttggt
    16141 aaccgcgcca taatcgaagt caccggccat cacagttatg tagtatattt ttcgctcctt
    16201 ctgagaaagg gccatagcca gagttgcata aggaaggttc atcggatcgc cgtcgcgaac
    16261 gccaggagcg gccatggaga caaggggcga taccgtccct tcctgacctt tatggggatc
    16321 gtacttaaag agcagattac tgccgccgat gatgccatag gccgcgcgat ccaacggatc
    16381 ccattcgatg atgcgccact gcgccttgcg gtccagcatg ggatttgcca tggtgcgcga
    16441 ctggttaatc agcggcaggc ggatgtgctc aaagtcaaaa atgcggtcct gttggatgtc
    16501 gtatttccag atgcgccccg gcggaaagct gccatacaca tttccgaggt cgtccgagaa
    16561 gatggtgcgg cagacatccc aactgtccac gcggccgagg tcttcggtcc agtccttgtc
    16621 aatgtggtac ttgtacaggt ggccattctc ggcaaggccg taaatgagcc tgcgcttagg
    16681 atccatggtc tgcccaagca ggccccagaa gctgttgatc aatccgagtt gctccacctt
    16741 tcccgttttc atattgatcc ggtaccagcg cggtcccttg tagctggcgg catcgatggc
    16801 cggcgggccg ttgtcctcgc tcaatgagcc gaagtacacc cagccgtcca actcctgcat
    16861 ctgaacgtgg atcttgccgt tcgtccaaat gcccttaccg cgttcctcgc tcacttcggt
    16921 caggttggcc aggtggcgca tctccccggt cgcaggatca aaccggtaga cattggctgc
    16981 gtcggcatgc gtgcacagtc ccacgtagat cttgccgtcg gagccgatat agagagcgct
    17041 ccacatctga gcgtctttat ccacggtgct gaagcggtag tgcttgatcg gcacgaattt
    17101 cgcccggtcg tccgcaaagc acgatagtgt cgagcacaac agaatgaagc ctgcaaacat
    17161 cgcacggaac atagcgcact ccttgaatcg gtctacctga cttcgcgccg catagcgtga
    17221 cggcggttcg gccacgaatt atacgcgtaa ctgccctgcg ctggctattc gatcacggcg
    17281 ccgtctccga cgtcttcgcc gatgcgaacc atgcgccctg ccggagcaac ctggttgttg
    17341 ctcaattgga tactgcggct tgcggcgccg gtcacccgca ggaaatcctg aacggcatgc
    17401 ggtgtgcggg cgccgcggac catggctcca acgacatcgt gaaaccacag cacggcttcc
    17461 ttgccatgca cgctatcggc gcggaaaccg tccagatcca attcgcggac atcgtggaag
    17521 atcatcgcgg gcctcacgtc gggctcctcc gtcctgagtt gcagactcct catggtcaaa
    17581 ccttccacgt ggcgcacgaa gaacccgtgc gacggcagtt tgccgaacat cctcgcctcg
    17641 gggtacttgt cctcctgctc cgggacgtcc aacagccccc ttacttcctg ctctccgcct
    17701 ttcatggtga tattgatgcc ggtgaggctt acgttctgca cgcgatgccc cggcaaaccc
    17761 gtgacagaac tgctcatcgt tccgccggcc gctatgacat tattgatcga aacattccgc
    17821 agactgccgg tagtgggaac cgcctgtccg cgtccgcgat tgcccagccg gatgaagatc
    17881 ggcgtgtcca catcccgcat ggtgatgttt gaaaccacca cgccgtcggt taccgcgcca
    17941 tccaccatct caacggcaat tccggagatg ggacgttgga ccgtgtccgg cttgccgtat
    18001 attgcgcagt tcgtcacgga tatgttgcgg aagtcccctc ggctctcggt gcccatcttg
    18061 aaggcgttgc accttgtggt cagaacacag ttggtgatgg tgatgttctc gctgggcagt
    18121 atcttgccgc ctcgttgtcc agtggtcttg atgacaatgg catcgtcgcc ggtatcgata
    18181 aacgaatcgg cgatcagaac gttgcggctg gagtccgggt caatgccgtc cgtgttggga
    18241 ccacgcgggt cggcgatgat ccgaatgccg cggatgagca cgccatcgca ctccaacggg
    18301 tgaatagccc accccggcgc ctgacgaata gacacatcct gcacgcgcac atttcgcgag
    18361 ttgacgaact caagaagcgg actcggcctt cccggcatcg ccttgtaaga gcgccgcccg
    18421 gtctgtttat cccaccaggt gtcgccgttt ccgttgatgg ttccctgccc ttccacggcg
    18481 atattttcgg cgtccaccgc gtagatcagg cggcgcgggt tgtaatcctc ggctcgtttg
    18541 ctcgcccaca gggtagcgcc cggatacagg cgcagcgaca cgttgctctt caaaacgagg
    18601 gtgccgctca ggtaattgcc ggccggaagg accacaacgc cgccaccggc gctgaaggcg
    18661 gcatcgatgg ctttctggat ggccggagaa tctaaagtga ccccgtctcc ctttgcgccg
    18721 tgcgcgcgca cgtcgaatgt ttgtgcggcg gcggccgcgg cgaacaacat cacgattgca
    18781 gacgttttca tgtgcgctcc tttacttccc atgaatatgg gtacgaccgc ccaatcgttc
    18841 gcgcgcattc cggaggtcgt tcccttcgag gaccaattcg tccgcggaaa ctccgcggac
    18901 ggaagcgaag gctcccgcgc ctgccgctgc gacgctgccg gagatgaaga cgttcttcgc
    18961 gtgatcgagt ccgaatacgg cgagagaacc gtccggtttc gcttgccggc cgtgaaagcc
    19021 ggaaacgatc aggtcctcca cgcccttcgc gtagacggca tgccgctcca ggagcggcgc
    19081 gtccgcggcc cacactaact taaggtcgcg cagatgcagt ccccgcacat tcgcgaagac
    19141 ccaagcggcg ggaacggaac tgtagtcggc ttccggctcc actctcccca cgttgctgct
    19201 gccccgcggc ttcgacatct tctccaccgg ttcgaagccg gtaacacgca tggttacgcc
    19261 gcgaaacgcc acgtcctcca cgggttggtc cggcatgccg ccgacaagca cgcgcccctt
    19321 cgtagccagc atgagatcgc tgaagttgat ccgcctgacc cggctctgcc gcgagtccgg
    19381 aaaacgcttt tccaaatcga tgtagatcgg gtactcagcg gaattcgggt gcgtggggaa
    19441 cgtctcgatg ctgatgttgg agaagctgac atcctcgaaa acgccgccgt ctttcgcata
    19501 gatcgccagg ccgacgcgcg tcttgcgaat tacgatattc gaaaacacga tgtggcggaa
    19561 gtcgccgtgg ctctcggtcc cgagtttcag ggctgcatcg tcgcttacga gcacgcaatt
    19621 gttcacaatc acgttctcgc aggctcgcgc cggcatgccg gccctcccgg tggtcttcag
    19681 cacgatgcaa tcgtcgccgg tctcgatata gctatcggcg atgatcacgt ggcggctgga
    19741 gtcgggatcg attccatcgg tgttcgggcc ctgcatgggg ctgatgatgg aaattccgcg
    19801 gatttcgacg cgctcgcaat tcagcgggtg aatgccccat ccgggtgtgt ttcgaatacg
    19861 aacgtcgcgg atgcggacgt ccctgcaatc cacgagttcg atcaaagggc tggggcgccg
    19921 cttatggggc ttgaaatccg ggtaccagaa cgcatcgccg tttccttcga tcctgccggg
    19981 accttcgatg ccgatgttct ccgcgccttt ggcataaatc aggtgattcg gctgataatc
    20041 ctctatcttg cggcttcccg ccaaggttgc gccgccgctc agatagagag tgacgttgct
    20101 cttcagtacg agcgttccgc tcaaaaaagt tccagcggga agatgtactg ttcccccgcc
    20161 ggcgcgcgcg caggcgtcga tcgcctgctg gatggccctc gtatcgttcg tcttgccgtc
    20221 gccggtggcg ccgtgggccc tgacatcgaa aacacctggg ggcgcctgct gagccgcggc
    20281 cgcggccgcg gcccagcaga cgaggcacat taaccaggaa gctttcatgc cgtttcctgg
    20341 ctaccaggaa agccgccctg cgagttgaat ctcgcgcggg ccctccgtcg tagaagtgat
    20401 gcggccgaaa ctggcattgg tgaccgtagt gttcggcgag cccaggtgcg tggtattgaa
    20461 ggcgttgtag aattcggccg agaaccggaa ctgaacacgc tccttaatcg gaaagctttt
    20521 cgcgagcgtg aaatcccact tcctggttcc ggccgcccgc agatccacga tgcgcgagga
    20581 gagctggcgc aaggtgaacg gctcctgtgg aacgaactgc gtcacgtcga accactcgtc
    20641 cacggtccgg gggttgtcct ccgaccggaa caggcgttct ccggcaacgc tgagggccgc
    20701 accggattgg aacgtcccca cccagttgag ctgccatcct ccgagaacat gtcccaccac
    20761 aggatgagcg ccactcagga accggcgccc ttttccgaac ggcatttcgt aaatgccatg
    20821 cacgacaaag cgctgcggcc ggtccgagtt ggagatcacg cgttccggct tggcgtcgtt
    20881 ggagttcagg tacgaagtcg cttccatgag cttcgatacc gtgtagatgg cgccgaaata
    20941 gaggccttgc gccatgcgcc gggtaacctg gagttgcatc gagtcgtaat gagaccggcc
    21001 ataaggcaga acggcctctg taagcccggt gaaatgcggg aagggccgca gcaactgcga
    21061 caccgtggtg gtccgttggc tcagggagct ggtcgagtct gtcaccacac cgtagaacgg
    21121 gttggggacg ctggcgttca gccgggtcct caatgataag tattcgttcg gatactggtt
    21181 caagccgcgg gtcatcgcaa gcctctgaga cacgttgccg gagtagctcg catccacgga
    21241 cacctgtgcc ggcaacacga actggaaacc catcgagaac tgttggctca tgccgttgag
    21301 atatctgggc acggtgcctg cgggcgctct acccgccgta aggctctgcc caacgcccgt
    21361 catcagcccg ttggcgtttc cgggcggcat gctcaggcca tttggaaatg gatcggaaag
    21421 caggtgaaaa ggagtgagat tgttgtcgag cgatgtgatc attcgcgtgg ctaggctgaa
    21481 accgtccgcc cggaagttac tcacgttgtt cggaacgtaa aacatgccgt aaccgccgcg
    21541 ccacacgatc caatcggtca cgcggtatgc aaagccgaaa cgcggcgcat acatgagcgc
    21601 ggacgtcttc aggtttcccc gcgggacgcc gccggtgttc aggaatccga gtccgccctt
    21661 taccgaaagg gacgcgagtt cgggaatcgg actctgggcg taatttgcgg ccgcggcgga
    21721 ttcgatggga ctggccacat cgaaatcgaa tcccgcgctt ccctggttga atcgttcagt
    21781 gacaggccct tcgtgctcga aacgaagccc caggttgagc gtcagccggt tggtggcctt
    21841 ccagtcgtcc tggacgtaga aggagaggta cgtattctgc agcgccatcg acgcattcat
    21901 atccgcaaac ccgctcgccg gggcgcccag gagaaaagac gccacgtcgt ggcccgccgt
    21961 cgtggtgctg cgcgttgggt ccggaccgcg ggtgaataca cggttaaagt cgaaccgtcc
    22021 gcttgagcgg cccggctgaa agctgttcga ctgcgcgagc tgataacgga atccgtattt
    22081 cagcgcgtgg cggttcatcg tggtgtggaa attgaccagc gggttgtact gattgacgac
    22141 gttcctctgc gcttgccggc cggccccgag atgctggacg tctccgctca tttgaaagcg
    22201 cggaaaattc gcgaaggcaa tcgaattcgc gaagctcgcc ggaaagccta gcgtcgcgat
    22261 gtcgaaaccc tcgccgaaca tgaactcgcc atcgaaatag cgcgtgaatc cggcgcggac
    22321 gtccatgacg atgcgcgggc tgacgatcca ggtatagccg gcgcccgccg cgatattgcc
    22381 gcgggcgaaa acatcctgtc cgctggtggg attgcccgcc gggcgggagg cttcgccata
    22441 gaagggggcg ctcgggtcgg ttcgatgatt ccatccgaac cgcaggaaga gattgtgcgc
    22501 cggcccgatc tgatggtcgc ctcgagcgga cacgctggcc catctgagct cgttcatatt
    22561 cgggatgtcg aaattctcca gattcgaagc tgtcggcgtg acgttcggca tcgggtagta
    22621 tttcatggcc gccacagcca ccgggtcaag gcggctggcg ggaatgatat tacccgggaa
    22681 cggagatctc acgtatccgg agccgctggg ctgcgtcgtg gccggatcga agatcgtcaa
    22741 cggctgcccg ccttgggcga acgagcgcgt gaagtctcca ttgcgctcca acgcagaggg
    22801 cacgatggct cgcgaccggc cgacgccgac gctgcgatgc ccctcgaagt tcgtgtggaa
    22861 atgggtgcgg tcgcggccgt catacagctt cggaagatac accggaccgc ccacgttgaa
    22921 accgtatgtg ttgcgcttgc gttcggcttt cggattcccg ttgcggttat tgaagaagct
    22981 gttcgcgttg aggttgtcgt tgcgatggaa ccaatagacg ctgccctgca gttcgtttgt
    23041 gccccctctg gtggagacgt tcacgaaggc cgcgcccgag tggccgtatt ccgcgtcaaa
    23101 tgcgttggtc tggacgcgca tttcctgcac ggcttccggc ggaggactga aactgaccgt
    23161 ctggatgcgc atgttcggaa tgccatccag gagaaattcc gaactgctct cggagccgcc
    23221 ggcggcaaaa tccgaaggcc cgttgttatc ccattggttg atgtcggccg agccgcgcat
    23281 gtgctgaacg ccggctgcca gcgtgaggaa agcgtatggg ttgcggctaa tcagcggcag
    23341 ttcggcaatg acctgcgtat tcaccgaagt gccgagcgcg ccggtttccg tgcgaagcag
    23401 cggactctcc ccggtgaccg tgacggattc ggtcagttcg cccacctcca ttgtcgcgtt
    23461 gagcgtggtg gtttcgccgg tttcaaggag aatgccgtcc cggacgaatt tcttgaagcc
    23521 tcccgattcg atttccagcc ggtacgtgcc aaagtcgagg agcgcgaacc ggtacaggcc
    23581 ggttacgtcg ctgcccgtct ctcgcaccag accggtagcg gtattgacga gccgaagctt
    23641 cgcgccaggt atcgcggaac cactggagtc gtaaacgacc cccgtgagat tgctcttgtt
    23701 cacctgtccg gacgcagatg ccaggaagca caaacctgcc atccccgcca acaaatattt
    23761 ccgaatgtgc atacgttttc acccctgcga ggtttcacac ctcagttcga aatcacgcga
    23821 tcgtttggac cgggaccgct gtcacatagc ccgcagtccg taaaaccctt cacgtgatgc
    23881 tggataatcg tttcgcccgc gccccatgac tcacggcgaa gacgctccta ccatttctgg
    23941 catcgattgt agatccgccc tgttgcacga tcaaggtact taaatgccct ttcatgaact
    24001 tttttggcgt cccgtccgct tcgccggggc tggtcgggta gtttaacgaa tcccaagccg
    24061 tgtgccctgt tgggcgtagc tctgtcaggc ctcagcataa cttccgttcc ttcgccgcca
    24121 catcgcgtat agcaacccaa gagttagcca ccctgcaccc aaaacctgtg cgacagggcg
    24181 cagattcagc cagatgtaaa agcaaaccag gaatccggca accggaggca gaacaaacgc
    24241 catgctccgg tttccgctgc ggagcgcgtg ccgcagtgcc gcaacattaa cgcccatgaa
    24301 cgcgatgaat gcgccgaaat tcagcagttc cgcggcgagt tggtaactga gcgcaaaccc
    24361 gccgatcacg cacagcgcgc cgaccagtag tacggcgttg gcgggcacgg cggcgcgcga
    24421 cagcgttgcg aagaacttcc gcggcagcgt attctcccgg cccatggcga acatgaggcg
    24481 agcggcgccg agttgcgcgc ccatgcccga tcccacgctg gcgaccaata aactgaaatt
    24541 gagcaacgca aacagccccg gcccgccgac tcgtcccccc acgtatacgt aagccgtgtc
    24601 ggcgtcggga aacgtagtgt agtccggcca gatcacttgc gcgatgtaaa cttcggcggt
    24661 cgcgagcacg ccggtgatgg cgcacgtcag taccgtggcg cgcaggatat tgcgtttcgg
    24721 gttctcgacc tcctccgata acgtcgagat cccgtcgaaa ccgatataag taagcgccgc
    24781 gatcgaggcg ccggtcagaa catccgaaga agaaaatgtc gctggattat agaatggcag
    24841 cagccaggcc cctgcttccg gtggggccgc ccagagataa cgcgcggccg caccaaagaa
    24901 aatcacgatt acaccaccaa gaccgcaggc gagaaacgta ttggtgcgcg ccgaagcttc
    24961 cacaccccgc aagttcagcg acgtgaacag cagggcaaag aagacaaccc agactccgta
    25021 cggaatggcc ggcaggaagt tacccgccgc tttcgaacac cagatggtgc agaggatcgg
    25081 attcagaatg tagtccagca tcatcgccca tccggtcgcc tcccccgcca gcgaatggat
    25141 ttcccggccg acatacgtat aagccgagcc cgccgacgga tagacgcgcg ccatacgccc
    25201 gtaggcgatg gcggtaaaca gcattgccac gagcgcgatc agcaccgcca ggactgcatg
    25261 gcctttcgac acctggctgg cgacaccgaa gagcggcatc ggcgcggtgg gctgaatcag
    25321 cacgatcccg tagaagacga gatggcgcag tctcaaaacg cggcgcagtg tcgtgggtgg
    25381 acttaccacg gtcacgatgt ctctctgcgt tcggcgaggg cggtcgtgac tttatcatac
    25441 gcggcaataa tgtcgtcttc gtccccctgt gtcaggcagg acggaatggg cagcatgagg
    25501 gtgcgctcga acaggtcgtc ggccaccggg catgttccgc gttcgtagcg gccaccgagg
    25561 ccctggttcg acggatggct ccatgggaac cgggcacggt cgacgctacc gcgccgcacc
    25621 agactggcga tgttgtagta agcgtgcagt ccaaagtcct tgagcaggat gttggccact
    25681 ccctggggat acgtgacgat cccctccgca cgcaatgcat cgcacgccgc cgcggcggtg
    25741 gcgccgtcgc ggaaaatggt cagcagaaaa caggacgtgt cgccggcgga gtcgacgagc
    25801 cgccgcagtc cgacctccgg cattccctga agagcggcgc gaatccgccg cttgctatga
    25861 cgcatcgcac cgacgatgcg aggcagtttt gccaactgca cccgcagcac cgcggcgcgg
    25921 atttcatcca tccggtaccc gcgtccccaa agcaggaggt ccgggtcgga ggtaacaaga
    25981 cggccggtct cgtcacgcat gtagccgaga tcgtgggagg caaacatgcg cgcgtagagc
    26041 cggtcgtcgt ccgtaattgc gcagccgccc tcgccggcgg ttatgttctt gttcatctga
    26101 aagctgaaca ccgcggcatc gccgaacgtt ccgacgttcc gcccgccaat cgtgccgccc
    26161 gcgcattgcg cgcagtcctc gagcagaaac agtccgtgcc ggcgggcgat gtcgagaatg
    26221 ctgcgcacat cgccgggcgc accgctcatg tgaatcatca caatgccgcg cgtgtgaggc
    26281 gtgatgcggc gctccacgtc cgccggatcg aggcagaagg tgtcgtcgat gtcggcgagc
    26341 acgggaactg caccgagatt caccactgcg gcagcgaccg agacccagag gaacgcggga
    26401 acgataatct cctgccccgg accggcgccg agtgcagcca gcgcgacatg cagcgcaccg
    26461 gtaccgctgc ttacggcaag ggcgtgccgc gcgcctgtga aagcgcggaa gtcggcttcg
    26521 aacctgtcgg cttcgcattg cagatcgacg ccgtagtacc ggaagagcga gcgcgagcgc
    26581 agcactcgca ccgccgcttc gatttcctgt tcgcctatgt ggtgaacgcc gggaaactcc
    26641 agcggcaacg gctccgtgcg gaccggggag cctccatcga tcgccaactt cggttcaacc
    26701 ttgggtagca tttgttttag cagaatagct cccgccggtt ctgccgaaaa gcaacttatc
    26761 caccatgtac agcacttaaa tgccctgcgg cgatcaaaca agtaccggga cagaacgata
    26821 tgtcactggc atcgccgccc cgcggcggct atctttgcaa gaagcatgaa aactcccctg
    26881 cttatcgcct gtacggctac cgcactgatg gttgcctccg ccaccgaaag cccgaagtat
    26941 ctggatccaa aacagcccgt gagcgtgcgt gtagaggatc tgctggctcg catgacgctg
    27001 gaagaaaaga tcggccagat gaacatgccc tgcgtgtacc tgccgcaact cggcggcggc
    27061 agatcttcgg gcaacagccg cgttctcgac gacaggattg tcgccgccag gatggaagga
    27121 acgaagcgct ttgcagcggg cgagtttgcc gaaggggtag gtcccggagg cggtttcttc
    27181 acgcttgcca acacggtgct ttatgaaggc ccgcgccagc aggcggagtt cttcaatgag
    27241 ttgcagcgca tcgcgcttcg gtcccggcta aagattccgc tgctgcagac cgaggaaggc
    27301 acgcacggct ttatggcgtc gggcgcaacg atctttccgg aaggtccggc ccttggcagc
    27361 acatggaatc tggatctgat tgaacgcata tacgcggcag tcgcacgcga ggcccgttcg
    27421 gtcggtgttc atcagcttta cacgctcgtg atcgagccga cgcgcgaccc gcgtctcggc
    27481 cggaacgagg aaggctacag cgaggacccg tatctggtat cacagatcgc cgggcgaatc
    27541 gtggccggca tccagcgcga cgacatttcc gcgcccgatg cagccattgc gggattgtgc
    27601 cactttccgg ggcagagcca gcccgtcgga ggactcgagc gtggccccat ggagatgagt
    27661 gaacgtgtgc tgcgcgagat ctttctgccg ccctgggaag ccgggatcgg gaaggccgga
    27721 gcactcggag tgatgctgac gtaccccgcc atcgatggcg tgccatcgca cgcatcttca
    27781 aagcttgtga agggcatact gcgcggggaa cttggcttcc gcggtctgac tctgggagaa
    27841 ggcgggggca tttctacgct tgtcgaggag cacgttgcgg ccgatgcgaa ggaagcggga
    27901 gagatcgcac ttcgctcagg tctcgacgtc gggatttctc tcgaacgggg atatctgaaa
    27961 ccgcttgcag acggagtgcg ggaagggcgc gttccgctcg cgctcgtgga cggcgcggtc
    28021 cgccggattc tgaccatgaa gttcgaactg ggccttttcg agaattcgct ggtcgacgtg
    28081 gaccacgcaa tgaagacggt acacacaccg gagcaccggc ggctggccct ggaagccgcg
    28141 cgggaaggaa tcgtacttct gaaaaacgac aagaacatgc tcccgctgcg ccgcgacctg
    28201 aaatccatcg cggtgatcgg accgaacgcc gataacatac gcaatcagct tggcgactat
    28261 atcgcgcgga aggtcacgac gcctgtgacc acggtgcttg gcggcgtgcg cgccatcgcg
    28321 ccgaatgcgc gcgtacagta tgcgaaaggc tgctctgttt tgggtgaaga ccggtcgggc
    28381 ttcgcggaag cagtgcgtat tgcgaaggag tcgaacgtcg caattgtggt agtcggggaa
    28441 aacgaacgtc actcacccga gggaggcacg aacggcgagg acctcgacgt agcgagtctg
    28501 gacctgacgg gcgtgcagga ggatctggtg aaggccgtgg ccgcgaccgg cacaccaacc
    28561 gtggttgtgc tggtgaacgg acgtccgctg tcgactcgct ggattgcggc gaacatcccc
    28621 gcggtgattg aggcgtggct tcccggtgag gcgggcggaa cggcggtggc cgaagttctg
    28681 ttcggtattt acaatccaag cggcaagctg gcggtgacgg tgccgcggca tgtaggccaa
    28741 ctgccgatct actacaactc gagtccgtcg cggcttccgg cggaacgcgg ccgaggaccg
    28801 gcgtggcggg gatatgtgga tttggaagga tcgccgctgt atcccttcgg ccacggcctt
    28861 agttacacca cgttcgaata ctcgaatctg cgcattgaac cgaagtccat gccgccgggc
    28921 ggaaccgttc gcatctctgt ggacgttgag aacagcggta agctggcggg cgatgaggtg
    28981 gtgcagttgt atcttcggga caacgtctcc tccatcgttc gtccaattaa ggagctgaaa
    29041 ggattccgta agattcatct tgctccggga gagcgccgca cggttacgtt cgacgtcacg
    29101 ccggaagagc tcgcgttcta caatcggcac cttgagcggg tcgtggaacc aggcacattc
    29161 actgtaatga ttggccgctc gtcggaagat attcctctga gcggcttatt tgaagtgcgt
    29221 tgaccgggtg ccggctgtac tccgctggcg acgagccaac aagcctgcgg aagacccggg
    29281 cgaaatagct ggcatccttg aagccgcact gccgcgcgac ggtcgagacg ttgtgcgcgg
    29341 gatccagcag aagccgccgg gcgagttcca cacgacggcg cataacatag cgctcgaatg
    29401 tatcgccagt ggtctggcga aacacgcggc tcagatgtcc cgtggagatg ccgagcgcgc
    29461 gcgccagtga actctgggtc atcgtcgtgc cttcggcaat gctgcgctcc acaagccggc
    29521 agacgcgatc caccaaacgg ctgcctttgt ccagatagat ttgccgcact tcatccagga
    29581 tggtctcggc ggctattgag tagccatcct gtacttcaaa cgagttgctc gcacgcccaa
    29641 gcgactcacc cgccttcttt ctgagctcgg cgagcacggc gggatcgcat ccgagactct
    29701 gccccgctga tagcatcgca ccgacggcgg agaggagaag gcgcttttcc agagcgagat
    29761 cggcgccgaa ctgccgctcc acatgcaacg gcagcgagtg tagagcgaga cgcgcctctt
    29821 caaaccgccg ctccttcagc agctcgggaa tctgcgccgc gagaacagtg gcttcgcact
    29881 ccggctcgga cgaagcgcga tacaccgcga tgggagccgt tgtcgttgat agggcgacgc
    29941 aggcttcgcg atacgactct cccagccggg cgaactcatt tgtgcttccg ccgattccga
    30001 cccgtacgtg tacggagctg cgctgggcga cggcatcgag gatgctctgc gccagttcct
    30061 gagcgttcag gcggccgacc tgcttgcgcg aacccggccg ctcgtaaagg aacatgcata
    30121 cgccgcgtcc ctgaagaaag gcgtgaacca cgttatcgag acgctcgcaa acctcttcaa
    30181 tcgcatgcag aatggccgtg aactcgcggt cggagtcgac tcgcaaagta ccctcgctca
    30241 tcggccaccg cgcaaccagg acgcggttcg ggcagttccg cagaccggtg cgggcaagcg
    30301 cggcccgggc gtcggccgtc tcagtggacg cgccggtcag aaccaggtat gcgaactcct
    30361 ttcgccggat ctcgagttcc cggtagtgtt gcctctcctg ctgcacggct ttgcacaggc
    30421 gctcccacga ggatgccagg tactcggcga agacttcgag aatgcccact gtggactgga
    30481 tgtcttcgtc ggacacaacg gcagagcgga agtacgcctc gcgcagttca ctgaggttga
    30541 tgtaagaaag gtgctctaca tctttgacga tttgcgcgaa gccctcttcg gtcggcggtg
    30601 agttcagcac ctgcccgctg aacagagtgg cgatgtgggc tccgtccgac aatacaggga
    30661 cggcgatgtc gagcagccca aaatgacatc gatagacttc cgcctttcca gttcgcgaga
    30721 cgcgctcttc ggccgccttg tccgaaacca ggcaggactg cgcttcccgc cggccgaggt
    30781 cgttgacgat gttgcagaag ggcggatgtg gattgcccgg agaacaatgg agaaggtgcg
    30841 aacagggacc gccgagcgcc tcgtttatac ccatgtggcg ccatcccgag cgtcccagtg
    30901 gtccgttctc cgtccacacc agatcgacgt tccggatagg acacgagcgg ttcatcatga
    30961 gcatgaaaac ctggaggaat ctcaggtcct tttcgaattc ctccatgaac aagtcccgtt
    31021 ccggaccgag gagcaacgcg ggcctggatg cggtggatcc agttggccgg ccttttccga
    31081 ttcgcgaatc gttaccgccg ccggacggat gcgtaaccag acgagtggaa ggaaacatga
    31141 gcgatcattc ctacgcgctt agctctcgtg agggcctcga tgggtttcgc accagccaga
    31201 gccgcgcacc gcactcgccg gccgctgctt gcgttcaacc ccgagcccac cccacatgga
    31261 ttcgttaacg gtatcacagc gtaattactc ccgacaagaa ggctcacgac atacaaacca
    31321 actcggcatg tcaactcggc attgacacgc ggctggatct ggaatatagt tcacacaagc
    31381 tgtatacact ccggaggggc gtgtatgaag aggggaattc tgctgtgctg ccttgcggcg
    31441 gtaagtgctc cgctctttgc ccagagcatc accggcacga tcctgggcac cgttttcgac
    31501 ccaaccaagt ccgttgtccc cagcgccaaa gtggtggtga cgaataccgc ccagggctgg
    31561 acccgtgaga cgacaacgga cgagaccggg agctacaccg tggcccaact acctcccgga
    31621 cgatatcagg tggacgtcag cgctccaggg ttccggactg tttcgatttc gaatatcgaa
    31681 ttgttggtgg accagcgtgc ccgcgtcgac gcggacttgc aacccggcac cgttaccgaa
    31741 cagattaccg ttacagcggg agcgccgctg ctcgaaaccg acagtaatgc ccttggccag
    31801 gtggtgaaca cgcgcaacat tcgtaacctt ccgctgaacg gccggcggtt cttcgatctt
    31861 gcgctgttga ccgccggcgc cgcaccgcaa ggaaccacgt tctcctccgt ggtgtggggc
    31921 cgagttacgg gcgtatcgct ggcgggcacg cgtgacatca acgtcagctt tctggtcgac
    31981 ggcgccgaga cgcgggatga gcgctatggc ggaacgttcc agttttcttc ggtcgaaagc
    32041 attcaggagt tcaaggtcca gcagaatttc gtagacgcgc agtacggcca tgcctccgcg
    32101 atggtttcgg cggtcaccgc atcgggaacc aacgaatttc acggcgcgct ctatcacttc
    32161 ctgcgcaaca ataaactgga cgcccgcaac ttcttcgatc gcggtgaagt tcctcctttc
    32221 cgcttcaacc agtttggcgg cagccttggc gggccggtct acctgccggg ttacagcggc
    32281 aaggaccgtt cgtttttctt cttcaattac gaagggcaga gacgccgcag gcaaaccacc
    32341 agcatcgcca ctgtagccac tccggcgcag agggccggcg atttgagcac actgccgctg
    32401 cagatctatg atccgctctc cggaaacgcc gccaccggcc gccgccagcc gttcgcgggg
    32461 aatcttattc ccgcggaccg catcgatccc attgcccggg ccctcatgga ttactggccg
    32521 caaccgaatc tgcccggcaa tgccgccaac tacatcacca ccgtgggcga gaaaatcgac
    32581 tacgaccagg ttacgacacg cttcgatcat cacctttcga gcaaagaccg cctgatgggc
    32641 cggtacaact acatcgacca gccgttcttc cggggcaact acgcgccact tgccggcagc
    32701 gtcgctccat tgcgcaacaa cggcgtcgtg gcgcagtaca caaggttggt gtctccgcgc
    32761 gcagtgaacg aattccggtt ctcctactcg cgctcctcgg cccagtttgc gcaggaaccg
    32821 gtcagcgaga atctggccgc tcaaatcggc ctgaagaaca catccacgga tcccaaagaa
    32881 tttggcctgc ccagcgtctc gatatcgggc tacaacgggt ttggatcgtt ttcgccgacc
    32941 atcatgaaca tcacggatcg cttccaatgg gcggacgact tcacgtatac acgcgggcgg
    33001 cataacctga aagccggcat cgacctacgg cgcctgcgct accggcagcg ctcggcacag
    33061 gacccccgcg ggttcatgca atatcagaat aatttcacca ctccggggcc cggcatcggc
    33121 ggtggcggcg acgcgctggc cgactatctg ttgggtactc ccggtttctg gcaggtacag
    33181 ctcgaggagt tgggcttcga cggtcgaatg attcagcccg gcatctactt ccaggacgac
    33241 ttcaaagtga cgtcccgcct gagcctgacg ctgggagtcc ggtgggagta caacagccct
    33301 tgggtacagc cgcgtaacaa catggccgta ttcaatttcg aaacacagca gcttgaatat
    33361 gctttgcggg acccgttcgc tttccgcacc agtaccgagc cgggcgacgt ggtgcgccgc
    33421 tccatcatca acccgcagta caacaactgg gccgatcgca tcggcctggt ctaccggttg
    33481 tttgagaaga ctgtgattcg cgcgggccat ggctggtatt ggaacaacgt caataacaac
    33541 cagctcacgc agagcatgag cctgttctat ccgttcgtat acaatccgca gaacacggaa
    33601 tcgaattcgc agctaacgcc cagcttcttc aacagcaact tgtatcccgc acggccaacc
    33661 ggtaccggat taccctacgg acccacattt gcgttcttca ccgtgcaaaa agatttcccg
    33721 cgtccctaca ccagccagtg gaacatcaat atccagcaga gcttcggcga gaattacatg
    33781 ctcgaaatcg gatacatggg aaaccagagc catcacatgc cgtcgtacac gaactacaac
    33841 caggcgcggc tgccggaccc gagcattcca ttcgcgcaac aaccgctaca gagccgccgt
    33901 ccgtacttga actatggcag catcaatatg ttcgatcgta tgggtaatgc aagctaccac
    33961 gggttgacgg cgaagctcga aaagcgcttc tcttcgggtt tgagttttct cgcctcctac
    34021 acctggagca aggccctgga tacaggcacc gacatatcca gtgacccgat caagcagcct
    34081 ggcgacgcca aatcctaccg aagcatggct tcgctggacg tcgggcaccg gtttgcaggt
    34141 agctattcgt atgagcttcc attcggcaga gggaagaagt tcctctcggg cgccggaggt
    34201 tttgccaatg cggttgtaag cggttggcag gtgaacggca ttaccgtgtt ctcactcggc
    34261 gtaccgttcg gcgtcagcgc tccgtcgagc attcctgatg tggacgcgcg tttcgttacc
    34321 gcgaatcgca cctgcgacgg ccaactgccg cgcggcgaac gtacccgtct gcggtacttc
    34381 gctaccgagt gcttctcgct gcccgcgccc ggaacgttcg gcaattccgc ccggaatctg
    34441 tggcatgggc cgggtatcaa caactgggat gtttccttct tcaagaattt ccgtttgtgg
    34501 aacgagcgca gcaatctcca gttccggttc gagtccttca acttcttcaa tcacacgcaa
    34561 ttcggcaacc cgtcgagcgg cttgccaagc ggcaccttcg gtgtgattaa cggcgcgaag
    34621 gcggcgcgtg aaaatcaact ggcgcttcga ttaagcttct gatcatgtcg agattttgcc
    34681 tgattgcagg cctgctccta caggtcccgt tattggtgtg cggacaggat acagtggccg
    34741 cgtttgcgga aaaggcgtat gccgccctgg ctgtcgaaga accgtataca ttccataaag
    34801 agctgagtga atggcgggag cccttgcggc gcgatgccgc cgcgcgcccc ggcgtatccg
    34861 agatgtcgat cggtcagaca gggtggcgtc tcctcgttcg cggcgattcc ggtctcgtac
    34921 tgcgtaccgc ggcggaagaa ttcagcgaat acctcgaacg ctccatgcag gtgaagattg
    34981 cggtggagcg cccggcgtcg ctggcgggat ggaacaacga gcggaacgcc atcatcgccg
    35041 ggacccgcga gcagcttccg ggttgtggcg aaagtttgaa ggggccgaaa gattaccgca
    35101 tcgccgtttc gcccgaccgc gtcatcgtct gcggctacga cgagcgcggg gccatgtacg
    35161 ggctgtttga catcgaacgg cgcatgaatc ttcgcgaagc gccgttcctg gaccgcacgc
    35221 tcgacatcac gcgccacagc ctgttcaaag ctcgcatggt gctttcgggc ctgggatggg
    35281 aggaatggcc ggaccgctac ctggcgctgt tggcgcgcta tggcttcgat tccattttcg
    35341 cttcggtata cgtgaatccg aacggcgtgc cgggtccccc gccctattgg gaccggatga
    35401 aaaaacagga cccgaagcgt gtacacgatc tgattcgccg ggccgcgcga tacggtatcg
    35461 atctctactg ccccatcatt tacctttata ccggtgagcc ggaaaacgag gcagggttgc
    35521 gcaagctggt gcgcgatatc gtgacggaat ttccgcagat ccgcggatac atcctgctca
    35581 ccgaaggctt tttctataga agcttcttcg gagcaggagg acagggcaat atcgatctgc
    35641 gggattgggt gcgccactgg gcgcgcggcg tggccatcgt ctccgaagag tgccgcaagc
    35701 ggaatcctgc cattgaggtc ctgccatggg aatacaacat cgattttcgt ccgaaccagg
    35761 tggacctgaa gcgctatgga accgcacagt tgccggtgga aaccattccg ctgctgacgt
    35821 tcgaaaacgg aaaggctttc gagcgggacg gcgagcaggg ctctctgaaa gattacgcca
    35881 tcaacgaagt gggtcccgcg gaggtcaccg tcgcgcagat cgatgaagcg cgcaagcggg
    35941 ggatgcgcgg tgtctattcg aaagctgaca cgttcgcgag ctggcaatat ggcacctttc
    36001 cctacctgcc gtttccctat cagtggcacg cccgctatat ggcactcgag aaatacaaaa
    36061 tcgacggcac gatggaaacg tggagctacg gattcaagcc cagttggatg gccgaagtac
    36121 gcgcctggta cagttggagc ggctacccgg aactgaatcg actgctcacc ggtatggcgc
    36181 gccgcgagtt cggagcggga aacgaagacc tggtgctcac ggcctggaag cgatttagcg
    36241 acgcgatcaa actggtgccg gacacgggac ccaacatggg caccaacaac tccgtcgcaa
    36301 cgccgttgtt cttcgaaaag ccaaaggctc gtgcaatgac gctcgatcat tcctgggagg
    36361 accccgcgat ctggcagcgg cattcccagc tctggccgta ttggccctac actccgagaa
    36421 ggctgatcct ggtacccgat ttcacgaatg aatccaatgc ggccgagcgt tatgcacgtc
    36481 cgttttcgct aggggtgttc aataaatatc tgacactcgc cgcggaccaa atggaacagg
    36541 gtcttcgcag ttatcgccag gcggcgctga aggctcccgc gtcaaagcgc gccggcgcca
    36601 tgcgagaggt tctgctcgcc gaacagcttc agcgcatgat acgcagcgac cgcgcggtgc
    36661 tggaattcga ggacatgcgc ttgcggctgg cgaagaccgg ggatcgcgcg atgcgtttgg
    36721 atttgctgaa gcgtatgacg gcgctgctcg aagaggaaag aacacgcacg caacactcgc
    36781 tggaaacagc gcgtcgggat tcgcgtttgg gttatgagtg ggagcaggat tacatctaca
    36841 cgccggatca catcgaagag aaactgaaac ttattgacct caccttgaag gagcagatac
    36901 cggcgtaccg caatcggtag ctgatgcacc gctcccttac ggtcacggct ccgtaacgca
    36961 acgatcctac ggagccgcgc gcaagcaagc gggcagaggc gctgcgccgc atgcaaacaa
    37021 aaccgctgtt agaacgtcag gcgaagcccc aactggattc gccgtgccgc gagagagttg
    37081 gtgatacggc cgaattgagc gtttgtattc acaccggcgg cattcggcga gaaggtgacg
    37141 ttcgggttcg tatggttgac gtgattgaag gcgttaaacg cctccgcgcg gaattccaga
    37201 ttggccctct cataaatcga gaagttcttg aaggccgaga gcgcaacgtc caccatgccc
    37261 ggaccgcgag tggtaggaag agtccgtggc gcgtttccga gcacgaaatt cggtggattc
    37321 cggaatgcgt ccgtgtcaaa ccacctctga accgatcgtt cggaggacgg cagcgtcgga
    37381 tcgcggacaa cgtccggcca attgatgccg gtgaagttgt tcgcgccgcg aacactgagc
    37441 ggcgttccac tctcgatggt gacgattgta ttcacctgcc atccaccggc cacatgtccg
    37501 agcacgcccc tggctccgcc cagcaacggc tttccgggac cgaatggcaa ctcgtagacg
    37561 gcacttccga ccaggcggtg agtaacatcg ttctggtcga tggcccgatc gagatcacgg
    37621 ttaaagcggc ccaggcggta ctcgccgatg gcgcctgaac tcccggcgga cgagaacgag
    37681 tcgttgatga gcttgctgtt ggtgtaagac accagcgcac tcagtccgct tgcataccgt
    37741 ttttcgatcg taacctggag cgagtgatag atgctcgagg agttgtgggc ggcgtacgtc
    37801 gtgatggagc cgtagtctgg gaagggttgg agcaacagcg agcgttgaac cgtcctggcg
    37861 gaaagagccc cggttgcgat ctgcccgaag aacggattct cgagctggtc ctgaagcccc
    37921 agtccgaatt gctgccaata gatcgggtcg agttggttca ggttgtagtt gccgccgaac
    37981 agcttggtgc ccttgttgcc cgcgtaggcg accgtgccca cccaccttcc gggcaattcg
    38041 cgctgaatgg cgagattcca ctgttgaaga tagggcgtcg gcgcattgcg atcctgatag
    38101 cggacgtttt gtccgcggaa ggcgctgggt ccgccggcgg gaccgagagg gacaatcagc
    38161 gcactggggc cttcgctgaa cttgaacgcg cggaacgggc ctccgcccgg agccacaaac
    38221 ggcgtggccg aggcaaagcc gaaggagttc gagttgtcgc cttgcgtgtc gccggattcc
    38281 accagcatgt agatgaggcc gtagccgccc cgaatagcgg ttttgccgtc gccggtaaca
    38341 tcgtaggcaa aaccgaaccg cgggccgagg ttgttgtaat cacgatccac gaagtgacgc
    38401 ggcgacgtga catcggcata tgtcagcaca ccgggcatat tggtctcggg attcatgatg
    38461 tacggatcga agttcgagaa acggttgtgc cgctccaccg gctccgacgc cagatcgtag
    38521 cgcagcccca gattgagcgt caggcgcggt gtgaccttgt aatcgtcctg gaagtacatg
    38581 gctgtcgacc aggagtgaaa cgagaacgcc ggcacgaact cctgcgttcc accggacacc
    38641 tcgccgagca gaaatgtagc catgcccacg ccagtgcccg ccggtctcag cgggtctccg
    38701 gtaagcccgg ccgtgaagct gaaattgccg gaccggttcc gcaattgggt gtagttcagg
    38761 cggaaccagc gttgatcaac gcccgccttg atctggtgct tgccttttac gatcgtgatg
    38821 ctgtcgccaa tctggatcgt gtgctgcgcg cgaattcccg cggcgaacgg atcagagccg
    38881 agcggcagca tgccgctgat accgacacgc gggaacaaat cgcgcgggaa gattgccggc
    38941 ataccgagtt ggtccggcca gtcccctcca aaactgggat gcaggaacgg caggttctgc
    39001 cgcgtgaagt tcgccttgaa ttcgttgatc acggccggcg agaagacgtg cgtctccgta
    39061 acgataacgt tgtgattatc gcgctggtcg cgccgggcaa aaatgtccgg gtcggcggga
    39121 cccaggcccc agccacgccc ctgacgcgtg ttgcgcgtca cggtgtagcg gcccaacaag
    39181 ctatctttgt cgctgaagcg ctggtcgata cggatgttgg tgacgccttg attcacgggc
    39241 gtggaagcca gcgacaggaa attcagagag ttc
//
LOCUS       DASTMK010000264         8737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_76163, whole genome shotgun sequence.
ACCESSION   DASTMK010000264 DASTMK010000000
VERSION     DASTMK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76163"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1568
                     /locus_tag="VFQ79_11410"
     CDS             414..1568
                     /locus_tag="VFQ79_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0140316.1"
                     /translation="MLSDLQKSALAQRTGIFLAVAGVAGLEWMAPQWLHDAAVVRLYF
                     LPVIWAALYEGWRGGVVIASISGFALMLHAFALRVAVPGSIFNHSTEAVWLYVIGALI
                     GIPAGRQRRQKEQYRSLADQLSSVYEKVQANFEGMKRAERLSAIGQLSAGLAHEIRNP
                     LASISGAAGILQRTQGLDSRGEKCLDIITNECRRLNGLLTNFLNFARPRAPHLQSVQL
                     DPILDNVIALASHAIRGKTVNLQKLTAQNLPPVECDPEQLEQVLLNLMINAIEASPEN
                     EPVTLSAGAAGTSVAVRVIDYGHGVAPADIDKLFDPFFTTKENGTGLGLSVAHQIVRQ
                     MGGSLVAERNPEKGMTFSVILPPKQTEYDSSSNSGSRRRRESPLGATDTT"
     gene            1498..2910
                     /locus_tag="VFQ79_11415"
     CDS             1498..2910
                     /locus_tag="VFQ79_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0140317.1"
                     /translation="MTPVRILAVDDDENLRWVLQTQLEEAGYEVETAADGEAALEILD
                     RALPALVLTDLKMPGLSGMDLLQKIRETWPELPVIIITAFGTIQSAVEAMRAGAYDYV
                     TKPIDYEELVLVVHRALEHFRLVEEVRTLRSSLDRKYGFENIIGHSEALLSLLDMAAR
                     AARSNSTILIRAETGTGKELLARAIHFNSPRREKPFITINCGAIPRDLLESELFGHVK
                     GSFTGAMGHKTGKVEMANHGTLFLDEIGEMPIELQVKLLRLIQQGEVEKVGAVSANKV
                     DVRIVAATHRNLQAMIEDGSFREDLYYRLAVIPLELPPLRERAEDIPELLQHFFALAK
                     EKLGRPDLVLPEALIPYFQNYRWPGNIRELENVVERLVVLSRGSEITVADLPEFLRRE
                     QPLADVLQLDLPPRGISLESIEKELLIRALEKFNWNQTHAARYLDISRKALMYRMEKH
                     GIRKKATGEDDGAEESEPGR"
     gene            complement(3009..5258)
                     /locus_tag="VFQ79_11420"
     CDS             complement(3009..5258)
                     /locus_tag="VFQ79_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140318.1"
                     /translation="MRNSICPALMLGLIPLAAFGQESGTLRGTVTLAATGDPLHHATI
                     TVSRAGRIAETGEDGKFEITGLPAGTYSILAHLQSLSDESKTITIAAGETVTVDFSLR
                     LSPLKQSLTVTANEREQSTFESFQTVTSLDSIELALKSKSSLGEVLEYQPGVAKRSFG
                     PGSSRPVIRGFDGDRVLVMQDGIPTSTLASQSGDHGESIDASSLDRLEVVKGPATLLY
                     GSNAIGGVVNAVTGHHLMHDHPHEGVRGYATGFGGSANGQAGGAAGFDAGFGNWLIWG
                     NGSGQRTGDYNTPIGTIENSATRVTNSAGGFGWFGAKGFFDAGYRYEEGEYGVPFAGE
                     LHGHHHEEAEDEHDHDHEGEEEHDHAEEHADIRLGFRRHNARFTTGWRNLDSFFQGFR
                     FSVNYADWEHKELENNQIATIFRNDQTTYRGVFTQRATGALSGSFGFQGSHRDYKTEG
                     EEALAPPVKQNNFAVFALEELAFERIRFQFGGRVENTRYKPDGLRDRSFTGFSGAAGF
                     HVPVAKNTAFVFNYTHSYRAPALEELYNHGPHVGNLTFEIGNPDLQRERSNGIDFSLR
                     HQSSRVRGEVNFFYYDIGDFVYLAPTGAEEDGLPEAEYLQNNSRFRGTELGLDLGLHE
                     NVWLNLGADYVDAELKNGGGPLPRIPPLRGRVGLEFRKNGLSIHPELRMAKDQDRLFE
                     NETRTAGYAVFNLGASYTITRQHMAHVFGLDAFNLGDRLYRNHLSFLKDRAPEIGRGV
                     RFIYTVRFF"
     gene            complement(5379..6134)
                     /locus_tag="VFQ79_11425"
     CDS             complement(5379..6134)
                     /locus_tag="VFQ79_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU0140319.1"
                     /translation="MIRALLVDDEPIARKVLREELEEVDDVEVIGEAGNGPQALEQIE
                     ALSPDLVFLDLQMPGMNGFEVVRNLEGSALPCIVIVTAFDRHAVEAFEAGAIDYLLKP
                     VGQDRLARCLERVRQLRKSTLAVAESVARLQEAAAQGPPARPRKIVGKLGEEYHLLDS
                     SHVLAFQAERELVWVVTRKQRYLATQPLKSIQAKLCGLNFARIHRNALVNLDRVSKMA
                     PLSSQRWLLTLDNQQEFIVSKRQARAVQKLLSW"
     gene            complement(6134..7984)
                     /locus_tag="VFQ79_11430"
     CDS             complement(6134..7984)
                     /locus_tag="VFQ79_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0140320.1"
                     /translation="MPGGLENSVNTLGHAAGVLIFGHFLVLSIWNQPAHRLRANRLAI
                     LAAALALLWNLASFAVLVSGDTGGNIEQVVAIAGFCVLSLLPAVLLHLCLASRFPLMV
                     RLGYVLSTISIGAHLLELTSDSALYHRAGLATITIGFGILTVASTAGILWPGGDHPRL
                     LSSRLLAGMSLFLFAISFVHFSEGQAHHAWSTELIFHHAGIPLALFVLLQDHRFVLAD
                     TFIRLLANGGVAGLLAFGLATASAHLNFVGQVLLGGMLLVIFGKASGPVQRFLSRMVF
                     RQKDPGTLLRDLRALASRTSDEVSYVEESSRLLAQFMGTEVIDIPQTLRESNLPGPVL
                     VTEVPESKELQRHGVQAVVPVRLTHGVTRSILLAGRVGGRRYLGDDLHTLARAAACVA
                     EEADHIRETEMRRLVTQAELRALQSQIHPHFLFNALNAIYGIIPREVKGARRMLLNLA
                     DVFRYFLQTDRTYVALEEELRIVKAYLAIEELRLGDQLRISIDVDESALGERIPVLSV
                     QPLVENAVKHGAAVCPQGGQVRIEIRREPGGLQVRVSDTGPGFAAEEMPGDPDRAGVG
                     LENVSRRLQLCYGRDAGISVENRMGWTTVSFFAPCPEIVRESTLRKKAGR"
     gene            8283..>8737
                     /locus_tag="VFQ79_11435"
     CDS             8283..>8737
                     /locus_tag="VFQ79_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140321.1"
                     /translation="MKKKNSLDQVQAYRLAEFRRPSFAAAEIIHATGLPEKTLQNYLA
                     RYEDAMQLSAPAPGKGQRRMYSAADAVYLAAVRAIANFGVAPSAAILLANHVRAWVMT
                     EARWRYECGFTGLPAQYFGFGPKTDGNGVDLMVQGAGPEIQSWAAEHGH"
BASE COUNT         1847 a         2584 c         2597 g         1709 t
ORIGIN      
        1 taactttcga gagagcggat ggccttgcaa ttgccccctc tataggcaac atgcggaggg
       61 attggatgaa acaggtcaca cgagacacaa aacacggaaa catctggcgg ggtcgcgcta
      121 gggggaggct gttatgctcc gatgcttgag gccaggctgc cctgacgcgt gcggcacgca
      181 ccatagaccc agttgtacgg accgcctcct ctattatgtg ctcctgattg cgttttgcat
      241 cgcgctcgcg gccagcttct ataccggagt tatacgaatt catgaaacgc gcaatgacct
      301 ctcgtcaagt cttccaacgg ctgggttcgt gaacgtaaaa tgattctggt tcaccggacg
      361 tgcgcggctg tacgctaaga agggtggcac accgcccgcg aacacttgcc tccatgttat
      421 ctgaccttca aaagtccgcc ctggcacaga ggaccggtat tttccttgcc gtagccggag
      481 tggcagggct cgaatggatg gcgccgcagt ggcttcatga cgcggcagtc gtaagacttt
      541 acttcctgcc ggtgatatgg gcggccttgt acgagggctg gaggggcggc gttgtaatcg
      601 catccatctc cggattcgct ttgatgcttc acgccttcgc gttgcgggtg gcggtgccgg
      661 gttcaatttt caatcattct acggaagcgg tctggctcta tgtcatcggt gccctgattg
      721 gtattcccgc gggcaggcag cgccggcaga aggagcagta tagaagcctg gccgatcagc
      781 tcagttccgt ctatgagaag gtccaggcga actttgaagg gatgaagcgg gcggagcggc
      841 tatcggcgat cggccagctc tccgccggcc tggcgcacga aatacggaat ccgcttgcca
      901 gcatttccgg cgctgctggc attcttcagc gcacgcaggg gctggattcg cgaggcgaaa
      961 aatgtctgga catcattacc aacgaatgcc ggcggctgaa tggcttgctt accaattttc
     1021 tgaatttcgc ccggccgcgc gcgccacact tgcagtccgt gcaactcgat ccgatcctcg
     1081 ataacgtcat cgcgctcgcc agccatgcca tccgcgggaa aaccgtcaat ttgcagaagt
     1141 tgaccgccca aaacctgcct cctgtggaat gtgacccgga gcaactggaa caggtgttgt
     1201 tgaacctgat gatcaacgcg attgaggcaa gcccggagaa tgagccggta acgctttcgg
     1261 ccggcgcggc gggcacgtcc gttgcggtcc gcgtcatcga ctacgggcac ggagtggcac
     1321 cggccgatat cgacaagctc ttcgaccctt ttttcaccac caaagagaac ggaaccggat
     1381 tgggtttatc cgtggcgcac cagattgtga ggcagatggg cggatccctc gtcgcggaac
     1441 gaaacccgga aaaggggatg acgttttccg taatcttgcc accaaagcag acagaatatg
     1501 actccagttc gaattctggc agtagacgac gacgagaatc tccgttgggt gctacagaca
     1561 caacttgagg aggcgggcta tgaagtagag accgcagcgg atggcgaagc cgccctcgag
     1621 attctcgacc gggccctgcc ggcgcttgtc ctgaccgact tgaagatgcc cggcttgtcc
     1681 gggatggatc tgctccagaa gatccgcgag acctggcccg aattaccggt gatcattatc
     1741 actgccttcg gcaccatcca gagcgccgtg gaggccatgc gggcaggcgc ctacgactac
     1801 gtaaccaagc ccatcgatta cgaagaactc gtcctcgtag tgcaccgcgc gctcgaacat
     1861 ttccgcctgg tggaagaagt ccggacgctt cgttcgagtc tggaccggaa atacggtttc
     1921 gagaacatca tcggccactc cgaagcgttg ttgagccttc tcgatatggc tgcccgcgcg
     1981 gcccgcagca attcgaccat cctcattcgc gcggagaccg gaaccggcaa agaactgctc
     2041 gcccgcgcca ttcatttcaa cagcccgcgc cgggagaagc cgttcatcac catcaactgc
     2101 ggcgccattc cacgcgatct gctcgaatcg gagctgttcg ggcacgtcaa gggatcgttc
     2161 accggcgcaa tgggacacaa gaccggcaaa gtcgagatgg cgaaccacgg cactctgttt
     2221 ctggacgaga tcggcgagat gccgatcgag ctgcaagtga aattgctgcg cctgattcag
     2281 cagggggagg tggaaaaggt gggggcggtg tccgcgaaca aggtggatgt gcgaattgtc
     2341 gcggccacgc accggaacct tcaggccatg atagaagatg gcagcttccg cgaggatctg
     2401 tactaccgcc tggccgtcat ccccctggag cttccaccgt tgcgcgagcg ggccgaagac
     2461 attccggagt tgcttcaaca cttcttcgca ttggcgaaag agaagctggg acgcccggac
     2521 ctggtgttgc cggaagcgct gattccgtat tttcagaact accgctggcc gggaaatata
     2581 cgcgagttgg agaacgttgt ggaaaggctg gtggttctca gccgcggcag cgagatcacg
     2641 gtggccgatc tgccggagtt cctccgccgt gaacagccgc tcgccgacgt gttacagctc
     2701 gatctcccac ctcggggcat cagcctcgaa tcgatcgaga aggaactgct catccgggcg
     2761 ctcgaaaagt tcaactggaa ccagactcac gccgcccggt atctcgacat cagccgcaag
     2821 gcgctgatgt atcgcatgga aaagcacggg atccggaaga aagcaaccgg ggaggacgac
     2881 ggcgccgaag aatcggaacc ggggcggtaa gtacgtggac accgctccct gacggtcgcg
     2941 gctcagaaaa gcgttgttga tagtgcggga cttgtgacag agccgcgagc gtgagcgagc
     3001 ggaccccgtc agaagaaccg gaccgtgtag ataaaccgca caccgcgccc gatctccggc
     3061 gcccggtcct tgaggaacga aaggtgattg cgataaagcc ggtcgcccag gttgaaggcg
     3121 tccaggccga acacgtgggc catgtgttgc cgcgtgatgg tataggacgc gccaagattg
     3181 aagaccgcgt atccggcggt gcgggtttca ttctcaaaca ggcggtcctg gtccttggcc
     3241 attcgcagtt cgggatggat gctgagaccg ttcttccgga actccaggcc cacgcgtcca
     3301 cgcagaggcg ggattctcgg cagcggccct cctccgttct tgagctcggc atccacgtag
     3361 tcggcgccca gattgagcca gacgttttcg tgaagaccaa ggtccaggcc caactcggtt
     3421 cctcgaaacc ggctgttgtt ctgaaggtac tcggcttccg gcagtccgtc ctcttccgcg
     3481 ccggtaggcg ccaggtaaac gaagtcaccg atgtcgtagt agaagaaatt cacttcgccg
     3541 cgaacgcggc ttgactggtg ccgcaaggag aagtcgattc cgttgctgcg ctccctttgg
     3601 agatccgggt tcccgatttc gaaggtcagg tttccaacgt gcgggccatg gttgtaaagc
     3661 tcttccagag ccggggcgcg gtaggagtgc gtgtaattga agacgaaggc ggtgttcttt
     3721 gccacgggga catggaaccc ggcagctcct gaaaagccgg tgaacgaacg gtcccgcaag
     3781 ccgtcgggtt tgtagcgagt attttcgacg cgtccgccaa actggaaccg gatgcgctca
     3841 aaggcaagtt cctccagcgc gaacacggcg aaattgtttt gcttcaccgg gggcgcaagc
     3901 gcttcctccc cctccgtctt gtagtcgcgg tgcgagcctt gaaatccaaa actgcccgat
     3961 aaggcccctg ttgcgcgctg cgtaaacaca ccccggtatg tcgtctggtc gttgcgaaag
     4021 atcgtcgcaa tttggttgtt ttccagttcc ttgtgttccc aatcggcgta gttcacagag
     4081 aaccggaagc cctggaagaa ggaatcgagg ttcctccaac cggtggtgaa acgcgcgttg
     4141 tgacgccgga atcccagacg aatatcggcg tgctcttcgg cgtggtcatg ctcctcctct
     4201 ccttcgtgat cgtggtcgtg ctcgtcttcg gcttcctcgt gatggtgccc atgaagctct
     4261 ccggcgaagg gtacaccgta ctccccttct tcgtagcgat atccggcgtc gaagaaacct
     4321 ttggctccga accacccgaa accgccggcg ctgttggtca cgcgcgtcgc ggaattttca
     4381 atcgtaccga tcggggtgtt gtagtcgccg gttcgctgac cgctgccgtt gccccagatg
     4441 agccagttcc caaaaccggc atcgaatccg gcggcaccgc ctgcctgtcc gttggcggat
     4501 ccgccaaagc cggtcgcata acctcgcaca ccctcgtgcg gatgatcatg catcaggtgg
     4561 tgtcccgtga cggcattcac cacgccgccg atcgcgttgc tgccgtatag caaggtagcc
     4621 ggtcccttga cgacttccag ccggtccagg ctcgaggcgt cgatcgactc gccgtgatcg
     4681 ccggattgcg acgccagggt gcttgtggga attccgtcct gcatcaccag cacccggtcg
     4741 ccatcgaagc cccggatcac cgggcgggag gagcccggcc cgaaactgcg cttcgccaca
     4801 cccggctgat attccagcac ctcgcccagt gaagattttg atttcagcgc caattcgatc
     4861 gagtcgagcg acgttacggt ctgaaacgat tcgaaggtcg actgctcccg ttcgttcgcg
     4921 gtcacggtga gcgactgctt caacggagac agcctcagtg aaaaatcgac ggtgaccgtt
     4981 tccccggccg caatcgtgat agtcttcgat tcgtcgctga gggattgaag gtgcgcgaga
     5041 atcgaataag tccccgccgg gagcccggta atctcaaact taccgtcctc tcccgtttcc
     5101 gcaatccgtc cggcgcggct gacggtgatg gtggcgtggt gcaacgggtc gcccgtagcc
     5161 gccagagtca cggtgccgcg cagagttccg gattcctgcc cgaatgcagc caatggtatc
     5221 agtcccagca tcagcgcagg acaaatggaa ttgcgcatga atctcctttt cgaagctgta
     5281 aaggggaacg atacctggaa tccaatggtg agccgagctt gcagttacca gcgggcggct
     5341 tgtagaaatg agcgggtgga ttccggaagt gagcggactt accagctcaa cagcttttgc
     5401 acagccctcg cctgcctttt gctgacgatg aactcctgct gattatccaa cgtcagcagc
     5461 catcgctggc tgctcaaggg ggccatcttg gaaacgcgat ccagattcac cagcgcattg
     5521 cgatgaattc tggcgaagtt caagccgcaa agttttgcct ggatcgactt caaaggctga
     5581 gtggcaagat agcgttgttt ccgcgttaca acccagacca gttcccgctc cgcctgaaag
     5641 gcgagaacgt ggctgctgtc gaggagatga tactcttcgc caagcttgcc gacgattttt
     5701 cgaggccggg ccggaggacc ctgcgcggca gcttcctgca accgcgctac actttcggcg
     5761 acagcgagag tactcttgcg gagttgccgt acgcgttcca ggcacctggc cagccggtcc
     5821 tggcccaccg gcttcaacag ataatcaatc gctccagctt cgaaggcttc gacggcgtgg
     5881 cggtcgaacg ccgttacgat gacgatacag ggaagggcag atccctccag attccgaacg
     5941 acctcaaagc cgttcatgcc gggcatttga aggtcgagga acacaagatc gggggagagt
     6001 gcctcaatct gttcgagcgc ctgcggtcca ttcccggctt caccgatgac ctcgacgtcg
     6061 tccacttcct ccagttcctc gcgcagcacc ttgcgtgcaa tcggctcgtc gtcgacgagt
     6121 aatgccctga tcatcacctt ccggccttct tccgtaacgt gctctcgcgc actatctccg
     6181 gacagggagc gaaaaagctc accgtcgtcc atcccatcct gttttcgacg ctgattccgg
     6241 catcgcggcc gtagcataat tgcagacggc gggaaacgtt ttccagccca acaccggcac
     6301 ggtccggatc gcccggcatc tcttccgctg cgaagcccgg cccggtatcg gaaacacgaa
     6361 cctgcaaacc tccgggttcg cgccttatct cgatccgcac ctgcccccct tgtggacaga
     6421 cggcggctcc gtgcttgacc gcattttcaa ccagcggctg tacggaaagc accggaattc
     6481 gctcaccgag agcgctctcg tccacgtcga tgctgatgcg aagctggtct ccgaggcgca
     6541 gttcttcgat ggcgagataa gccttcacaa tccgcagttc ttcctcaagg gcgacatacg
     6601 tcctgtccgt ttgaaggaag tagcgaaaga cgtccgccag gttgagcagc atacggcgcg
     6661 cccctttcac ttcccgcgga atgatgccgt agatcgcatt gagggcattg aacaggaaat
     6721 gcgggtgaat ctgcgattgc agcgcccgca gctccgcttg tgtcaccagc cgccgcatct
     6781 ccgtttcgcg tatgtgatcg gcctcctccg ccacacaggc agcagcgcga gcaagtgtgt
     6841 gaagatcgtc gccgagataa cggcggcctc ctacccgccc ggcgagcagg atgcttcgag
     6901 tcaccccgtg cgtcaatcga accggaacga cggcctgtac tccatgccgt tggagctctt
     6961 tcgattccgg aacttccgtg acgaggaccg gacccggcag atttgattcc cggagggtct
     7021 gtggaatatc gatcacctcc gtgcccatga attgagccag cagccgcgag gactcctcca
     7081 cataagagac ctcatcggac gtccgtgaag ccagcgcccg aaggtcccgc agcaacgtac
     7141 ccgggtcttt ctgccggaat accatgcggc ttaaaaaacg ctggaccggc ccgctggctt
     7201 tgccgaaaat caccagcagc atacccccga gcagcacctg gcccacgaag ttcagatgag
     7261 ccgaagccgt cgcgagacca aaggccagca ggcccgccac tccgccgtta gcgagcagtc
     7321 ggataaacgt gtcggcaagc acgaaccggt gatcctgcag aagcacgaaa agcgcgagcg
     7381 gtatgcccgc gtgatggaag atgagttcgg tagaccaggc gtgatgggcc tgcccctcgc
     7441 tgaagtgcac gaaggaaatg gcaaacagaa atagagacat gcccgcgagc aggcgggagc
     7501 tcaacaagcg cggatgatcg ccgcccggcc acagaatccc cgcggttgat gccacggtaa
     7561 gaatcccgaa tccgatcgtg atggtagcga ggccggcgcg gtggtacagg gcagagtcgc
     7621 ttgtcagctc gagcagatgc gccccgatcg agattgtact cagcacgtac ccgaggcgaa
     7681 ccatcagcgg aaaccggctc gctaggcaaa gatgcaagag taccgcggga agcaggctca
     7741 ggacgcagaa tcccgcgatc gcgaccactt gttcgatgtt tccgccggtg tcgccggaca
     7801 ccagaacggc gaaggacgcc aggttccaaa gcagggcgag tgccgcggcc agtatcgcaa
     7861 ggcgattggc gcgcaggcga tgggccggct gattccagat cgacagtaca agaaaatgac
     7921 cgaagatcag tacgcccgcg gcatggccaa gagtatttac actgttttca agtccgcccg
     7981 gcacactctt ccctcccgct ttgattctcg ccgatgcgcc cggcgcattg gattttcaga
     8041 tcgtttggga ttggtggacg gcatgggact cgaagttcct aacgaattga aaacaagcaa
     8101 gttacatatt ctaaagaacc ccaaaaaccg agaattccca gaataacaga ctcaaggtac
     8161 acctgggtac aaacgctgga aaacggccga attccgactg gcgaaaaaga gtgttgacaa
     8221 cacactcgcg tgagtattat cattgtcgtg tgatgcaaaa gagaatcggt ggagggttta
     8281 gggtgaaaaa aaagaacagc ctggatcaag tgcaggccta tcgattggcc gagttcagac
     8341 ggccgtcctt cgcggcagcg gagatcatcc acgcaaccgg gctgccggag aaaacgctcc
     8401 agaactacct ggctcggtac gaggatgcaa tgcaactctc ggcgcccgca cccggcaagg
     8461 ggcagcgccg aatgtattcc gctgctgatg cggtttatct cgcggcggtg agggcaatag
     8521 ccaatttcgg ggtagctccc tccgcggcaa ttctgctggc aaatcacgtc cgcgcttggg
     8581 tgatgactga ggcgcgatgg aggtacgagt gcgggttcac cggcttgcct gcccagtact
     8641 tcggcttcgg tcccaagacg gatgggaacg gcgtagatct gatggtccag ggcgcaggtc
     8701 cagaaatcca gtcgtgggcc gcagagcatg gacacga
//
LOCUS       DASTMK010000265        20409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_172681, whole genome shotgun sequence.
ACCESSION   DASTMK010000265 DASTMK010000000
VERSION     DASTMK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20409
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172681"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1060)
                     /gene="larB"
                     /locus_tag="VFQ79_11440"
     CDS             complement(314..1060)
                     /gene="larB"
                     /locus_tag="VFQ79_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEU0140322.1"
                     /translation="MDQEHLRELLEGVSAGAVGVDAALERLRHLPFEDLGFAKVDHHR
                     ALRHGMPEVIFAKGKTPEQVFSIAERVLENASNLLVTRCDTPTAELLAAKFPEAEYFP
                     LSSAIRVWRDRTLRGKGTIAVVCAGTSDIPVAEEAQLTAEVMGNEVDTIHDIGVAGIH
                     RLMGNRERLSKARVVIVCAGMEGALPSAVGGLVSVPVIAVPTSIGYGASFHGLAALLG
                     MLNSCASNVTVVNIDNGFGAGYVASLINRL"
     gene            complement(1076..1528)
                     /locus_tag="VFQ79_11445"
     CDS             complement(1076..1528)
                     /locus_tag="VFQ79_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU0140323.1"
                     /translation="MLRAIAVAAFALLVSCSEKKTVTMDEFTGRIVTLPNGTEILAEV
                     MMHPTDMMRGMMFRDSLPEGRGMLFVHRTPGQHSYWMYQVRIPLDIIWLDGTKNVVEM
                     SPDTPPCKAQSSRDCPSYGGNQNAQFVLELPGGSIAKHGIKLGDRLSF"
     gene            1564..2457
                     /locus_tag="VFQ79_11450"
     CDS             1564..2457
                     /locus_tag="VFQ79_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEU0140324.1"
                     /translation="MPQSFWKHFRLTLEMIKFEHSVFALPFALTGALLAAREDGLLTR
                     GLGWKLVWIVVAMVSARSAAMSFNRVLDAGLDAKNPRTQMRHLPARLLSVRFAWGFIV
                     ISSLVFLLAARALNPLCFALAPVALGILFFYSFTKRFTSFSHIVLGFCLGMAPAAAWI
                     AIRGSLNPRILWLTAAVTFWTAGFDIIYACQDYEFDRREGLFSLPRIMGIAGALWVSR
                     IFHVAMLGCLAMLAWTLSLGTLSIAGIVAVALLLAYEHSLVKANDLTRVDAAFFTMNG
                     WVSMLFFFFWAADIIFLRRGA"
     gene            2454..3581
                     /gene="mqnE"
                     /locus_tag="VFQ79_11455"
     CDS             2454..3581
                     /gene="mqnE"
                     /locus_tag="VFQ79_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEU0140325.1"
                     /translation="MTKLQVIIDDARLKPILDKVNSGERLSFEDGIALYRTPDLLALG
                     YMANQVRERLHGNVTFFNVNRHINPTDVCVASCRLCAFGKKARDPKAYTMSLDQVWET
                     AGKGWSESATEFHIVGGLHPELTLDWYCEMLRGLKQRYPQVHLKAFTMVEVAYLARRT
                     KVSVRETLERLRDAGMDSMPGGGAEIFCERVRRIICDHKIDGNEWIEVARTAHQLGIR
                     SNCTMLYGHIENEEDRVDHLMRLRALQDETGGFVTFIPLAFHPANTPLEHIPATTGFA
                     DLKNIAVARLMLDNIPHIKAYWIMMTPRIAQIAQRFGSDDLDGTVVEEKIYHDAGATT
                     SQSLRRSELLRLIREAGREPVERDTLYRPVERTETTFTVLV"
     gene            3640..4548
                     /locus_tag="VFQ79_11460"
     CDS             3640..4548
                     /locus_tag="VFQ79_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0140326.1"
                     /translation="MSIRPVKRLIQSKPTLEGAGVHLRRAFGFGNTSDFDPFLLLDDF
                     RNDVPEDYLAGFPWHPHRGIETITYVLAGTVQHGDSMGNHGTIGAGDVQWMTAGSGII
                     HQEMPKGDEAGRMHGFQLWANLPASLKMTQPRYQEVKVGEIPEITDDDGTHVRVVCGT
                     FWGATGPVDGTAADPIYVDVSVPPGRKRTFPVEITRHAFAYVFAGNGKFCNASGPLTV
                     PTEAVGWWNTTPPTEADNRSLVLFDRGDEVTVQAGEDGIRFLLVSGKPFGEPVAWYGP
                     IVMNTQEQLRQAFDELDRGTFLKSRT"
     gene            4570..5907
                     /gene="trmFO"
                     /locus_tag="VFQ79_11465"
     CDS             4570..5907
                     /gene="trmFO"
                     /locus_tag="VFQ79_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU0140327.1"
                     /translation="MRSPIHIIGGGLAGSEAAYQISKAGLSAVVYEMRPTRPTPAHKT
                     DQLAELVCSNSLKSEAEHSAPWLLKQELRRLDSLLMRVAGTHRVPGGHALTVDRDLFS
                     HEVSRTVAALPGIEIRREEVTRIPGDGITIIASGPLTSDALAGDIARLTGSERLYFYD
                     SISPIVSAESIAIPPAFHASRYGKSMDGTDDYLNCPFDKEQYERFVDALLSASSYEPK
                     IPEDATPFFEACLPLEELARRGRDTLRFGPMKPVGLTDPRTGRRPYAVVQLRQENLRA
                     ESYNLVGFQNHMRFGEQERVLRLIPGLENAEFIRFGQMHRNTYINAPAILNGTLQVKH
                     APQVLFAGQISGVEGYVESIATGLMAGIHAVALARGEQPAALPRETALGSLCHYISGA
                     DLANYQPANITFDLLPALDDETKRRLRHDKRARHAEVCRRAAAKLEEYMAVHV"
     gene            5900..6784
                     /locus_tag="VFQ79_11470"
     CDS             5900..6784
                     /locus_tag="VFQ79_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEU0140328.1"
                     /translation="MSDLHDSIERYLESLKRENASAHTLRNYRSDLKQLAKFLGEGTD
                     VRSLDVLAFRRWLVGLHGSKLGVATIRRKLAAARSLCRFLEREGAIETNSAKVVRTPK
                     APRKLPKVPTTAHMASLLDTVAEGKLDRPHPARDLALFELLYGSGLRVSEAVGLNLGD
                     LDLAERWLDVRGKGRKERQVPFTSRTAKALERYLPERKAEKSEHALFVNHRGKRLSTR
                     GVHKIVRLYAVLLMGDTSLHPHSFRHAFATHLLSEGADLRSIQELLGHASLSTTQKYT
                     EVSLTDLMAVYDRSHPRA"
     gene            6848..7225
                     /locus_tag="VFQ79_11475"
     CDS             6848..7225
                     /locus_tag="VFQ79_11475"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0140329.1"
                     /translation="MVPWDVEFSDEFEKWWNTLTISEQEDVAAYVGMLEEAGPGLRYP
                     YCSGVAYSRHARMRELRVQHQGRPYRVLYAFDPRRTAILLIGGDKTGKDRWYEEYVPV
                     ADRIYDEHLNGLRKEEESNGKEV"
     gene            7209..7541
                     /locus_tag="VFQ79_11480"
     CDS             7209..7541
                     /locus_tag="VFQ79_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU0140330.1"
                     /translation="MAKKFSELRARMPAASQERARKKTRVLRREMALNELRAAKEITQ
                     KQLADTLKVNQAAISKIERRTDMYVSTLRNIIAAMGGRLEIYAAFPDGKVSINQFAAI
                     DEPVPAEL"
     gene            7586..10102
                     /locus_tag="VFQ79_11485"
     CDS             7586..10102
                     /locus_tag="VFQ79_11485"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0140331.1"
                     /translation="MRTILGILCAVSAVAQQSGNAVAYPAAKHGGQYMQNYYMPPAPS
                     TTPWAPAWSPDGSWIAVGMQGSLWKVDPTSGTATELTEGAKYYSSPTFSPDGKWLVYT
                     ADDDARSIQLEVLNLETGQSHALTNGDGIYLDPVFSPDGTRIAYVSTRPNGYFNIFVR
                     GIRDGRWSGEEIALTRDNRYPRDRLYFGVWDMHTQPAWSRDGSEVFFVSNRGVPLGSG
                     DLMRMPVQEDGILKAKPVLQEQTLYRTRPDVSIDGKRILYSSTSGTADQFNHLYVLPV
                     YGGAPYKLTFGDYDDFHPRWSPDGEWIAYISNEGGLPQLYLLETYGGARKRVAIRERR
                     WKRPAGKLHVRVLDASSGALTLARIHGVAADGRFYAPVADYSRIGRSGLHSFHTAGEY
                     SVEVPVGKMRVQAVKGFQYLPAAADVEIRPGQVTELRLKLERPERLVWKGWRSGSTHV
                     HMNYGGNLHNTPENLLRMARAEGLDIVFNQVANKDNRVLDYQYFVPGGGEHPASKGSP
                     DVKLLIGQEYRPPFYGHMSFLGLRDHLISPFTTGYEGTAIESLYPSNTDMLRKARAQG
                     ALTGYVHAFSGDRDPLETSLGVGKSFPVDAALGTVHTLEWSAASRAALQVWHHVLNND
                     FQVAPVGGEDSISNLHRVKLVGSVRTYAYTGPDTSVEGWLEAAKKGLTFFSTGPLLDL
                     RINGSRPGEAVQLPPGGGAITIEGTAESIAPLSKVVLYHNGRVFKELPLSGRFRETVQ
                     VTGSGWFSLYAEGPHTPLLDGEYPQAATNAIRVYVGDRKIRNRASAEYFVRWIDKLES
                     MADAWPWWRSEKERKHVLAQFAEARAIYKARADESVGETR"
     gene            10113..11393
                     /locus_tag="VFQ79_11490"
     CDS             10113..11393
                     /locus_tag="VFQ79_11490"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEU0140332.1"
                     /translation="MEFDYAFEMATSNIRFGPGVTREAGADLSEWGVRRVMVVTDPYV
                     ASLAPVATVLESLNDNHVAFSLFDRVRVEPSDASFREAIEYARQGDFDAFVAVGGGSA
                     IDTAKATNLYSTYPPSDFLDYVNPPIGKGLPVPGTLKPLIAVSTTAGTGSETTGVAIF
                     DYSPLHAKTGIAHRRLKPTLGILDPENTRTMPPEVAASTGLDVLSHAIESYTAIPYQC
                     RPRPGKPVLRPAYQGSNPISDLWSLEALRLVAGYLQCAVEDPSDDEARSKMLLASSYA
                     GMGFGNAGVHLPHGMSYPVSGMVRDFKPHGYPVDHPLVPHGISVILNAPSVFRFTASA
                     CPERHLTAAEVLGADIRGVKPEDAGRVLSDRITSFMERLRVPNGLSALGYSREDIPAL
                     VEGTLPQHRVTKLSPRPAEKDDLAILFEQALVAW"
     gene            11405..12730
                     /locus_tag="VFQ79_11495"
     CDS             11405..12730
                     /locus_tag="VFQ79_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0140333.1"
                     /translation="MQTGTKANGMTREEVILAGKEFLFPSVFHYYREPLVIERGKDQY
                     VWDADGKQYLDFFGGIVTVSVGHCNEHVNQRVHAQIDRLQHVSTVLATEPQVRLARKI
                     ASVTPGGQLTKSFFTNSGTEANETAILLARAYTGSQEIIALRHSYHGRSAVAMALTGN
                     AGWRLGGALQPGIVHAHNAYCYRCPFESTYPECGVRCARDLDELIRTSTSGRIAGMIA
                     EPIQGVGGFITPPKEYFEIVAGIVRKYGGIFISDEVQTGWGRTGGKWFGIEQYGVSPD
                     VITGAKGLANGFPIGVTTAKPEIADSLRGLTISTFGGNPVTTTAAEAVLEFIEEQNLA
                     ANCTETGAYLRARLEELRAKHPLIGEVRGMGLLQAIELVRDRASKEPAVQEAARVMDA
                     ARERGVLIGKGGLLGNVLRISPPMNIGKADVDEFTRILDASLSEAGRPS"
     gene            complement(12725..13990)
                     /locus_tag="VFQ79_11500"
     CDS             complement(12725..13990)
                     /locus_tag="VFQ79_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0140334.1"
                     /translation="MPGHSRRTFLERFASGTAFYAGAATTLSGLNRALAAAPEGEAYW
                     QTIRRQFDFDESHIPMNAANLCPSPRVVAERVTQLTRDIDMDCSSQNRAKFGRLLEET
                     RGKVARQLGVTADEIALVRNTSEANNTINAGVPLKAGDEIVLWDQNHPTNNVAWDVRA
                     ARYHLAIKRVSTPRQPADADELVSVFERAFTPKTRLLAITHTSNNSGLRLPVRELCEV
                     AHRRGIYVHVDGAQTWGALHVNLREMGCDSYSASGHKWYVGPKEVGLLYVRASRIPEV
                     WPHTVAPGWGDDAEPDVKGARKFESLGQRDDACLAALGTAADFHDMIGPSRVEARVLQ
                     LASTLKKRLAGAGVKLATPSHPSLSAGVLVVEVPAANRQTVIDRLYKEFGIAAATPGG
                     LRLCPHLYNTLEHLDRAVEGVKALRGLLS"
     gene            14171..17734
                     /locus_tag="VFQ79_11505"
     CDS             14171..17734
                     /locus_tag="VFQ79_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140335.1"
                     /translation="MGNLIKSSSLIFIVALIISATIPATLSAQVLYGSVTGTVTDGTG
                     AVVPAAKVVLVNDETGLRREVVTDQVGIYRVVDLPQGPYTLEVSAGGFQSYKKTNIRV
                     VTGQVNSEDVPLAVGSPAQEITVQGTAAVLQTQKADVRTEITGHAIRNLPLNVYRNYQ
                     AVTLLAPGVFSTSAIADAYPNGLASGPERSMSIYSNGLPSRINNNRVDGATSINIFLP
                     DHMLIIPPAETIQEVNVQTANYSVEKGLTAGAATDVITKSGTNEFHGSLYAFHTNNAL
                     VARNFFDFSPKKAKRIHNNDGVTLGGPILRNKLFFFGNWDGYFERKATAGTGFLAPME
                     YRRGDFRGTLGSPLFDNQGQPVTVCTTEGGSTQLRQGMVFDPLTGDRTNGTGRCVFSH
                     NGQINVLPPNRIVQGAQNFWPRLTAPSIPGTVTSNTPYNHAYNQNNSFNRNIGIAKVD
                     WNRNDRHTIWGKWNLQDSLVITPLTFGDAGGPSGFDGDIITQIGTIGHTWMLSPSTVL
                     TGHFGLARQKTDTLNGLSGEPLGQTVLGVPGMNEPLDDVRYTGMPAIGMAGWTALGNA
                     DGSTPYVRRDWTYTISQNLTHMRGRHEFRMGIDLGANYLNHFQPEVCCIRGRMDFAQG
                     ITSINLPANAAAPATGQYMELYTSPTPSSATLAGRGYSAFLQNSVAAFNLGLFSQAQK
                     SIQYIDFTAHSWQYGLYFGDRFKATSRLTLDLGLRWEYYPMVRRSGAFTFDRYDPDTN
                     TLLLGGMGGNPDDLGVTTSKKLFTPRVGIAYRIGDNTAVRAGYGIAIDSMPMERPMRG
                     FYPQVIGATYFNPSTFVSGFLPYADFATGIPVLEGPDISSGKLIPPGNVTIHFVPQGE
                     FKRGYVQSWNFSIERKLPAEILLNVGYVGNHFVHEMNGRDINAAPLGTGSAGQPLAKY
                     GRFITTPSYEGYLDSKYHSLQVSVSRRAARGLFLQGSYTWSKTLAYSDDNTYGNALRF
                     NCPPSPAMPEGCLELNYGPTSFDRTQMVKAAFVWELPFGSGHALSSSSRAVNAIIGGW
                     QLNGIFTAITGAPLQIGQSRNGLNTPGTPQNPYVMRQPEYIKEQASFDAYSGIYWFNP
                     DAFVPNFTNNEIGNIPRRLSWLRGPGITQLDASIFRNFRWKERFTLEVRAEAMNVTNS
                     THYSDPNVTCTVVGNACRGSFGQISSAFGQRIAQLGAVVRF"
     gene            17742..20132
                     /locus_tag="VFQ79_11510"
     CDS             17742..20132
                     /locus_tag="VFQ79_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0140336.1"
                     /translation="MRRAIPALLLLATAAGVMPVAAQDASTERRKRDLDHLLKVLLPS
                     RTPATGRINAHDKTWEEWIRRTGALPPDFDSMPSHSELPDPLLMYENGRARPVKTAAD
                     WERQKKWLREQVSKYMFGYMPPAPGNVRGVITGTEKEGDVTIRHVRLEFGPEHRGTLR
                     VQLMIPPGQGPFPVFMTNHPRTRPWVATAVRRGYIGCIYYAADPIYGNDDDSDKFIEL
                     YPEYEFPVLARWAWAGMRAIDYLYTLPEVDKEKIALSGHSRNGKQALLAAAFDERIGA
                     VIPSSGNSGECDPWRYTTDMFANESIQLLAGAQPHWFSPHLRFFSGREDKLPVDQHTL
                     MALVAPRGLMPYTAYSEGASNPVGFEEGYRSAKRVYDFLGAGNNIWLHLRAGEHPTAA
                     GDIENFIDFTDTIFKRKSFPKFETFTHGYTFEGWRNLTGETVNPLKYPKRQVGETLKS
                     VTSAQAWQEKKESLRRNLLWALGEEPAGIRFPARRELGGRAPMTNPGWLAGLFNRPTP
                     NGPLAARLNKDGVGVADIGFGDDLPATLFYPLGEDGKPKSGKLPVVIWLHAYPYQHGW
                     SASSPWSSTGANYGLERRPSFPELVKRGFAVLAFDQIGFGTRVLDSARFYERYPKWSL
                     MGKMVADTRAAIEAVRALDIIDPDRVYLLGYALGAKVGLMTAALDGGVRGVASIAGVD
                     ALRLSTPDKGTEGLRHYSHLHGTLPKLGYFIGYEDRVPFDYDEVLAMVAPKPTLVVAP
                     TRDWYARIADVQREIQAAAPVFRLLGRELDVLTPDDFNRFPVRLQQQVYDWLAKLP"
     gene            20284..>20409
                     /locus_tag="VFQ79_11515"
     CDS             20284..>20409
                     /locus_tag="VFQ79_11515"
                     /inference="COORDINATES: protein motif:HMM:NF045213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0140337.1"
                     /translation="MSGHSKWATIKHKKAALDAKRGKVFTRIIKEIMIAARSGGDP"
BASE COUNT         4004 a         6316 c         6080 g         4009 t
ORIGIN      
        1 cgagctcttc ctgccgggaa tctggcgctc cggcgagccg tacgtgtcga cttgcagata
       61 ccgatgtcct tggccgtcgg cgacgatcga gtaggtgcac tcctactcga ttcgacgaac
      121 tcgtcaaacc gacgccggtc cagcaacagg ttcaggcgtt cgtacaacgg gtagctcgcc
      181 ggccggggca gcgacgccgt cgcgccccac aacttacttg tgccacgggc tgcccggcga
      241 gttctgccgc ggctgaagct cggcgccgat acgttcgaaa ggcgctatta ttgcatctac
      301 gattgacgtg cggtcacaac ctgtttatca ggctcgccac gtatcccgcg ccgaagccgt
      361 tatcgatgtt gaccaccgtg acgttgctgg cgcaactgtt caacatgccg agcagagccg
      421 ctaagccatg aaaactggcg ccgtagccga tgctggtggg gacggcgata acgggcaccg
      481 ataccagacc tccgaccgcg ctcggaagag ctccctccat gccggcgcaa acaatcacga
      541 cgcgcgcttt cgaaagccgt tcgcggttgc ccatcagccg gtgtatgccg gcaacgccga
      601 tgtcgtgaat ggtgtccact tcgttgccca tcacttccgc tgtaagctgt gcttcttcgg
      661 ctaccgggat atcgctggtg cccgcacaga ccacggcgat cgtgccttta ccccgcagcg
      721 tgcggtcccg ccaaacccgt atcgcactcg atagaggaaa gtattccgct tcgggaaatt
      781 tcgcggcgag cagctccgcg gtaggggtgt cgcaccgggt caccaggagg ttgctcgcgt
      841 tctccagcac gcgctcggcg atggagaaca cctgctcggg ggtcttgccc ttggcgaaga
      901 tcacctccgg cattccatgc cgcagtgccc gatgatgatc gaccttggcg aagcccaaat
      961 cttcgaatgg cagatgccgc aagcgctcaa gcgcggcatc cacaccgacc gcgcctgcgc
     1021 tcaccccttc aagcagctct ctcagatgtt cctgatccat accgccttca ggctatcaga
     1081 acgagagccg gtctcccagc ttgatgccgt gttttgcaat cgagccgccc gggagctcca
     1141 gcacgaactg cgcattctga ttgccgccgt aactgggaca gtctctcgac gattgtgctt
     1201 tgcagggggg agtatcgggc gacatctcga cgacgttctt ggtgccgtcg agccaaataa
     1261 tatcgagcgg aatgcggacc tggtacatcc aatagctgtg ctggccgggt gtgcggtgca
     1321 cgaacagcat gccgcgtccc tcgggcagcg aatccctgaa catcattccg cgcatcatgt
     1381 ccgtcgggtg catcatcact tccgcgagaa tctcggtccc gttgggcaat gtgactatac
     1441 ggccggtgaa ttcatccatc gtgaccgtct tcttttcgct acaggagacg agcagcgcaa
     1501 aggccgcaac ggctattgct ctgagcatga aataagatgg tagcgtaaca tccccgattg
     1561 ttcatgccac aaagcttttg gaaacatttc cggctgacgc tggaaatgat caagttcgag
     1621 cattccgtgt ttgcgctgcc gttcgcgttg acgggcgcgc tgctggccgc gcgcgaggac
     1681 gggctcctga cgcgggggct tgggtggaag ctggtgtgga tcgtagttgc aatggtgagc
     1741 gcgcgatccg cggcaatgtc gttcaaccgc gtgctcgacg ccggcctgga tgcgaaaaat
     1801 ccccggacgc aaatgcggca tctgcctgcg cgcttgttgt cggtgcggtt tgcctggggt
     1861 ttcattgtga tcagcagcct ggtctttctg ctggccgccc gcgcgctgaa tccgctctgc
     1921 ttcgcgctgg cgcccgtggc gctcggtatc ctttttttct attcgttcac caagcgtttc
     1981 acgtcgttct cccacatcgt tctcggattc tgccttggga tggccccggc ggctgcctgg
     2041 attgcgatac ggggttcgct caatccgcgc atattgtggc tcacggcggc ggtgaccttc
     2101 tggaccgccg gattcgatat catctacgcc tgccaggatt acgaattcga ccgccgcgag
     2161 ggtctgttca gccttccaag aatcatggga attgcaggtg cgctgtgggt ctccagaatt
     2221 ttccacgtgg caatgctggg ttgcctggca atgctggcat ggacactgag cctgggtacg
     2281 ctcagcatcg ccggtattgt tgctgtggca ctgcttctcg cttatgagca cagcctggtg
     2341 aaagcgaacg acctgacccg cgtagacgcc gctttcttca ccatgaacgg ctgggtgagc
     2401 atgctattct ttttcttttg ggccgcagac atcatctttc tgcgcagagg ggcgtgacga
     2461 agttgcaggt gattatcgac gatgctcgcc tgaagccgat cctggacaaa gtgaactcgg
     2521 gcgagcggct cagttttgaa gacggcattg cactgtaccg tactcccgat ttgctggcgc
     2581 tcggctacat ggcgaatcag gttcgcgagc gcctgcatgg caacgtcacg ttcttcaacg
     2641 tcaatcgcca tatcaatccc accgacgtct gcgtagccag ttgccggctc tgtgcattcg
     2701 gcaagaaggc gcgcgatccg aaagcgtaca ccatgtctct ggaccaggtg tgggagaccg
     2761 cggggaaggg ctggtcggaa tcggcgaccg agttccacat cgtgggcggc ctgcacccgg
     2821 agttgacgct ggactggtac tgcgaaatgc tgcgcggcct gaagcagcgt tatccgcaag
     2881 tgcacctgaa ggcattcacc atggttgagg tggcgtatct tgccaggcgc acgaaggtct
     2941 ccgtacggga aacgctggag cggctgcggg atgcggggat ggactccatg ccgggcggcg
     3001 gagcagagat tttttgcgag cgcgtgcgcc ggatcatctg cgatcacaag atcgatggaa
     3061 acgaatggat cgaagtggcc cggacagcgc atcagcttgg tatccgctcc aactgcacga
     3121 tgctatacgg ccacatcgag aatgaagaag accgcgtcga tcacctgatg cgcctgcggg
     3181 cattgcagga cgagaccggc ggatttgtga cattcattcc tcttgcgttt cacccggcca
     3241 acacgccgct ggagcacatc ccggccacaa caggattcgc cgacctgaag aacattgccg
     3301 tcgcgcggtt gatgctcgac aacattccgc acatcaaagc ctactggatt atgatgacgc
     3361 cgcgcatcgc gcagatcgcg cagcgcttcg gctccgacga ccttgacggg accgtggtgg
     3421 aagagaagat ctaccacgac gcaggcgcca ctaccagcca gtctttgcgg cgaagcgagc
     3481 tgctgcggct catccgtgag gcaggccgcg aaccggtgga gcgcgatacg ctttaccggc
     3541 ccgtagagcg caccgagacc acgttcaccg ttctcgtgta gtgttcggcc cgcggctttc
     3601 ccgtcagact acggtagact gtcgataatc ggagggtcca tgtctattcg gccggtcaag
     3661 cggctcattc aatccaagcc cacgctcgaa ggcgccggtg tacatctgcg gcgtgctttc
     3721 ggcttcggga atacttcgga cttcgatcct tttctgcttc tcgacgattt tcgtaacgat
     3781 gtccccgagg attacctggc cggctttccc tggcacccgc atcgcgggat cgagaccatc
     3841 acgtacgtgc tcgccggaac ggtgcaacac ggcgatagta tgggcaacca tggcaccatc
     3901 ggcgcgggcg atgtccagtg gatgaccgcg ggaagcggca ttatccatca ggagatgccg
     3961 aagggcgacg aagcgggccg gatgcacggc ttccagttgt gggcgaacct cccggcgtca
     4021 ctcaagatga cgcagccccg ctaccaggag gtgaaagtgg gcgaaattcc ggaaatcacg
     4081 gacgacgacg gcactcacgt tcgtgtcgtc tgcggaacct tctggggggc gaccggcccg
     4141 gtggacggca ccgccgcgga cccgatctac gtggatgtct ccgttccccc cgggcgaaag
     4201 aggacgtttc cggtcgagat cactcgccac gcgttcgcct acgtttttgc aggaaatgga
     4261 aagttctgca acgcatccgg tccgctcacg gtaccgaccg aagccgtcgg atggtggaac
     4321 acgactcctc cgacggaggc agacaaccgc tcgcttgtgc tctttgaccg gggcgatgaa
     4381 gtcactgtac aggccggaga ggatgggatc cggttccttc tggtttctgg aaagccgttc
     4441 ggggaacctg tggcgtggta cggccctatc gttatgaaca cgcaggagca actgcggcag
     4501 gcgttcgatg agctcgatcg cggaaccttt ttgaaatccc ggacctgaag accgggcgat
     4561 aatggggtgt tgcgttcgcc catacacatc atcggcgggg gcctggcggg ttccgaagcg
     4621 gcgtatcaga tctccaaggc gggccttagc gctgtagtgt acgagatgcg cccgacgcgc
     4681 ccgactccgg cgcacaagac ggaccagctt gccgagctgg tatgcagcaa ctcgctcaag
     4741 agtgaggcgg agcattccgc gccgtggctg ctgaaacagg aactgcggcg cctggattca
     4801 cttttgatga gagttgccgg tacgcatcgg gtgcccggcg gccacgcgct cacggtagac
     4861 cgggacctgt tctcgcacga ggtgtcccgt accgttgccg cgctacccgg aatcgagatt
     4921 cgccgcgaag aagtgacccg catacccggc gacggcatca cgatcattgc gagcggaccc
     4981 ttgacgagcg atgcgctggc tggtgatatc gcacggctca ccggttccga acggctctat
     5041 ttttacgaca gcatcagccc cattgtttcg gctgaatcca tcgcaatacc gccggcgttc
     5101 cacgcttcgc gctacggcaa gtcaatggat ggtaccgacg actacctgaa ctgcccgttc
     5161 gacaaggagc aatacgaacg attcgtggat gcgctgctct ccgcttcgag ctacgagccc
     5221 aaaattccgg aagacgcaac gccttttttc gaagcctgtc ttccgctgga agagctggcg
     5281 cggcgcggac gggacacgtt gcgtttcgga cccatgaagc cggtcggcct gacggacccg
     5341 cgcacgggga ggcggcccta cgccgtggtg cagcttcgac aggagaatct gcgcgcggag
     5401 agctacaacc tggtgggctt ccagaaccat atgcggttcg gcgaacagga gcgggtgttg
     5461 cggctgattc caggtcttga gaacgccgag ttcatccggt tcggccagat gcatcgcaac
     5521 acttacatca acgcaccggc gatattaaac ggcaccctgc aagtgaagca tgctccgcag
     5581 gtgcttttcg ccggacagat atcgggagtc gaaggctacg ttgaatccat cgcgacgggt
     5641 ctgatggcgg gtattcacgc tgtggcgctc gcgcggggcg aacagcccgc ggccctgcct
     5701 cgtgaaaccg cgcttggatc gctctgccat tacatctccg gcgcggacct ggcaaattat
     5761 cagccagcca acataacgtt cgatctactg ccggcgttgg atgacgaaac gaagcggcgc
     5821 ttgcgccacg acaagcgcgc gcgccatgcg gaagtctgcc ggcgcgcggc cgccaagctc
     5881 gaggagtaca tggccgtaca tgtctgatct tcacgacagt atcgaacgct acctcgaaag
     5941 cctcaagcgc gagaacgcgt ccgctcacac gcttcgaaat tatcgcagcg atctgaaaca
     6001 gctcgccaaa ttcctgggag agggaaccga tgtccggagc ctggatgttc tggcattccg
     6061 ccgctggctg gtcgggctac acggctcgaa actgggtgtc gccaccatcc ggcgcaagct
     6121 tgcggccgcc cgctcactat gccgctttct cgagcgggaa ggagccattg agacgaatag
     6181 cgcgaaggta gtgcgcactc cgaaagctcc acgcaagctg ccgaaagtgc ccaccaccgc
     6241 tcatatggcc tctctgctgg acaccgtggc ggagggcaaa ctggaccggc cgcaccccgc
     6301 gcgcgacctt gccctgttcg agttgctcta tggctccggt ttgcgggtga gcgaggccgt
     6361 cgggctgaac ttgggagacc tcgatctggc ggaacgctgg ctggatgtga gaggcaaggg
     6421 acggaaagag cggcaagtcc cgttcacatc gcgcacggcg aaagctctgg agcgatacct
     6481 gccggaacgc aaagcggaaa aatccgagca cgctctcttc gtgaatcacc ggggcaaacg
     6541 cctcagcacg cgcggcgtgc acaagatcgt gcggctgtac gcagtcctgc taatggggga
     6601 tacttcgctg cacccgcaca gctttcgcca cgcttttgct acccacttgt taagcgaggg
     6661 agcggacctc cggtccattc aggagttgct cgggcacgcc agcctttcca cgacacagaa
     6721 gtacaccgag gtttcgttga cagacctgat ggcggtgtat gaccgttcgc atccgcgagc
     6781 gtagcggcac caaaagcaac agtgcttcat tcccttgtgt ttatattcca tccatggaat
     6841 actgtagatg gtgccttggg acgtcgagtt cagcgatgaa ttcgagaagt ggtggaatac
     6901 gcttactatt tccgagcagg aggacgtggc ggcttatgtc ggcatgctcg aagaggccgg
     6961 ccccggtctg cgatatccct actgttccgg ggtcgcatat tcgcgccacg cgcggatgcg
     7021 cgagcttcgc gtgcagcacc aaggccggcc gtaccgggtt ttatatgcgt tcgatccgcg
     7081 gcggacggcg atactgttga tcggcggtga caagacggga aaagatcgct ggtatgagga
     7141 atacgtcccc gtcgccgacc gtatctacga tgagcacctc aacgggttgc ggaaggagga
     7201 ggagagtaat ggcaaagaag tttagtgaat tgcgcgcgag aatgccggcc gcttctcagg
     7261 agcgcgcccg gaagaagacg cgggttttgc ggcgcgagat ggccttgaac gaactgcgtg
     7321 cggcaaagga aatcacgcag aagcaacttg cggacacgct caaggtgaat caggcggcca
     7381 tatccaagat cgagcggcga actgatatgt acgtcagtac gctgcgcaat atcatcgcgg
     7441 ctatgggcgg ccgccttgag atctacgcgg cattccccga cgggaaagtt tccatcaacc
     7501 agttcgcggc catcgacgag ccggtcccgg ccgagttgta ggacaccggg aggtagaatg
     7561 tctgacgtac gtataatcat tagctatgcg cacgatactg ggaattttgt gcgccgtttc
     7621 ggcggtggcg cagcagtccg ggaacgcggt ggcctatcct gccgcaaaac acggcgggca
     7681 atacatgcag aactactaca tgccgccggc gccttccacc acgccgtggg ctcccgcctg
     7741 gtcgccggac ggatcatgga tcgcggtggg gatgcagggt tcgctttgga aggtggatcc
     7801 cacctccggc accgccaccg aactcacaga aggagcgaaa tattactcgt ctcccacgtt
     7861 ctcccccgat gggaaatggc tggtttatac cgccgatgac gacgcgcgca gcattcaact
     7921 cgaggtgttg aacctggaga ccggccagtc ccacgcctta acgaatggcg acgggattta
     7981 cctggacccg gttttctcgc cggacggcac acgcatcgct tacgtatcta cgcgccccaa
     8041 cggctatttc aacatcttcg ttcgcggcat tcgcgatggc cgctggtcgg gtgaggaaat
     8101 cgccctcaca cgcgataatc gctatccccg ggaccgtctg tacttcggag tgtgggacat
     8161 gcatacccag ccggcatgga gccgggacgg cagtgaggtc ttcttcgttt cgaaccgcgg
     8221 cgttccgctc ggctcggggg atttaatgcg gatgcccgtc caggaagacg gcattttgaa
     8281 agcgaagcct gttcttcagg agcagactct ctaccgtacg cgcccggatg tctccatcga
     8341 cggcaagcga atcctctatt cctccacctc gggaaccgcc gatcagttca atcatcttta
     8401 tgtccttccg gtatacgggg gagcacccta taagctgacg ttcggcgact atgacgactt
     8461 ccatccacgg tggtcgcccg atggagaatg gatcgcatac atctcgaatg aaggcgggct
     8521 gcctcaactc taccttcttg aaacctacgg cggtgccagg aaacgcgtcg ccatccgcga
     8581 acgccgctgg aaacgcccgg ccgggaagct gcatgttcgc gtcctcgacg cctcctctgg
     8641 tgcgctcaca ctggcgcgca ttcacggagt ggcggccgat ggccgcttct acgccccggt
     8701 ggcggattat tcgcgtatag gccgctccgg cctgcacagc ttccacaccg ccggcgagta
     8761 ttcggtagag gtgcctgtcg gcaaaatgcg agtgcaggcc gtgaagggct ttcaatacct
     8821 gccggcggcc gccgatgtcg agatacggcc tggacaggtc accgaactcc ggttgaagtt
     8881 ggagcgtccg gagcgcctcg tgtggaaggg atggcgcagc gggtccacgc acgttcatat
     8941 gaactacggc ggaaatttgc acaacacgcc ggagaacctg ttgcgcatgg cacgggccga
     9001 gggcctcgac atcgtgttta accaagtggc caacaaagac aaccgggtcc tcgattacca
     9061 gtacttcgtt cccggcggag gagaacaccc cgccagcaag ggcagcccgg atgtgaagct
     9121 gctgatcggg caggagtacc ggccgccgtt ttatgggcac atgtcgtttc tcggcctgcg
     9181 cgatcatctg atctctcctt tcaccaccgg atatgaaggc acggccatcg aaagccttta
     9241 ccccagcaat accgatatgc ttcgcaaagc gcgtgcccag ggtgcgctca ccggctatgt
     9301 ccacgcattt tcgggggacc gggacccgct cgaaacctcg ctcggtgttg gaaaatcgtt
     9361 tcccgtagat gccgcgctgg gcaccgtcca taccctggag tggtctgccg cttccagggc
     9421 tgcgcttcag gtgtggcatc acgtattgaa taacgacttt caagtcgcac cggtcggcgg
     9481 cgaagattcg atttcgaatc tccaccgcgt aaagctcgtt ggcagtgtcc gaacgtacgc
     9541 ctataccggc cctgacacca gcgtggaagg ctggctggag gcggcaaaga aaggactcac
     9601 gttcttttca acgggtcctt tactcgacct gcgaatcaac ggaagccggc cgggcgaagc
     9661 ggttcaattg cctccgggtg ggggcgctat cacgatcgaa ggtactgccg agtccattgc
     9721 accgctttcg aaagtggtgc tctatcacaa cgggcgcgtc tttaaggaac tgccgcttag
     9781 cgggcgtttc cgcgaaaccg tacaggtgac cggcagcggc tggttctccc tctatgccga
     9841 aggcccgcac acgccgctgc tggatggcga gtacccgcag gctgcgacga atgcgatccg
     9901 cgtgtacgtg ggcgaccgca agatccgcaa ccgcgcctcg gccgagtact ttgtccgctg
     9961 gattgacaaa ctcgaatcca tggccgatgc ctggccctgg tggcgttcgg aaaaggagcg
    10021 caagcacgtg ctcgcgcaat tcgccgaagc gcgtgcgatt tacaaagcgc gcgctgacga
    10081 aagtgttggc gaaacccgat agaggcagct tgatggaatt cgattacgcg tttgaaatgg
    10141 caacctccaa catccgcttt ggcccgggtg tcacgcggga ggccggcgcc gatctgtcgg
    10201 aatggggtgt ccggcgcgtc atggtagtga cagatcccta cgttgcttcc cttgccccgg
    10261 ttgccacggt actggaatcg ttgaacgaca accatgtcgc gttctcgctg ttcgaccgcg
    10321 ttcgcgtcga gcccagcgac gcttccttcc gcgaagccat cgagtacgcg cgccagggcg
    10381 atttcgacgc ctttgtggct gtgggaggag gatcggcaat cgataccgcg aaggccacga
    10441 acctctattc gacctatcct ccatcggatt tcctggacta tgtgaacccg ccgatcggga
    10501 agggactccc tgtgccgggg acgctaaagc cactgatcgc ggtttctacc acagccggaa
    10561 ccggcagcga gacaacagga gtcgctatat tcgactattc cccgctgcac gccaagacag
    10621 gaatcgccca ccggcggctg aagccaactc tcggaattct cgatcccgaa aacactcgca
    10681 cgatgccgcc ggaggtggcg gcttccaccg ggctcgacgt tttgagccac gccatcgaat
    10741 cgtacacggc cattccctac cagtgcaggc cgcggcccgg caagccagtg ctgcggcccg
    10801 cctatcaggg ctcaaacccc atcagcgatc tgtggtctct cgaggcgctg cggctggttg
    10861 ccggatatct gcagtgcgcc gtcgaagacc cttcggacga cgaagcgcgc tcgaaaatgc
    10921 tgctcgcctc ttcctatgca ggaatgggat tcgggaatgc gggcgttcac ctgccgcacg
    10981 gcatgtccta cccggtttcg ggaatggtcc gcgatttcaa gccgcacggg tatccggtgg
    11041 atcacccgct ggtgccgcac ggaatttccg tgatactcaa cgcgccttcg gtgttccggt
    11101 tcacagcttc tgcctgcccg gaacgccacc tgaccgcggc tgaagtgctc ggcgcggaca
    11161 tccgcggtgt gaaaccggaa gatgcgggcc gggttctttc ggatcgcatc acgagcttta
    11221 tggagcggct tcgcgtgccg aacgggcttt cggcgctcgg ctatagccgt gaagatattc
    11281 ccgcgctggt cgaaggcacc ttgccgcaac acagggtgac gaaactgtcg ccgcgcccag
    11341 ccgaaaaaga cgatttggca atattgtttg aacaggcatt agtcgcctgg taaaagggag
    11401 gaacatgcag acgggcacaa aggcgaacgg gatgacgcgc gaagaggtga ttctggcagg
    11461 caaggaattc ctcttcccat ccgttttcca ctactaccgc gagccactcg tgatcgagcg
    11521 cggaaaagac cagtacgttt gggatgcaga cggcaagcag tatctcgatt ttttcggtgg
    11581 catcgtcacc gtcagtgttg gacactgcaa cgaacacgtg aatcaaaggg ttcacgcgca
    11641 gatcgaccgg ctgcaacatg tatccaccgt gctcgctacg gaaccgcagg tacgccttgc
    11701 gcgtaagatc gcatccgtga cacccggcgg ccaactgacg aaatcgtttt tcaccaatag
    11761 cggcacggaa gccaatgaaa cggccattct gctcgcacgt gcctataccg ggtcacagga
    11821 gattatcgct ctccggcatt cgtatcacgg acgttccgcg gtcgcgatgg cgctgaccgg
    11881 aaacgcgggt tggcgcctgg gaggcgcgct ccagcccggt atcgtccacg cccataatgc
    11941 gtactgctat cgatgcccgt tcgaatccac ttatcccgag tgtggtgttc gctgcgcgcg
    12001 ggacctcgac gaattgatcc gcaccagcac atccgggcgc atcgcgggca tgattgcaga
    12061 acccattcag ggcgtcggag ggttcatcac gccgccgaag gaatactttg agatcgtcgc
    12121 aggcattgtc cggaaatacg gtggcatctt catcagcgat gaagtgcaga ccggctgggg
    12181 acgcaccggc ggaaagtggt tcggaatcga acagtacggc gtgtcgccgg atgtcattac
    12241 cggcgcgaag gggcttgcga acggatttcc catcggcgtc accacggcca aacctgaaat
    12301 tgcggattcc cttcggggac tgaccatctc tacgtttggc ggcaatccgg taactactac
    12361 tgcggcagag gccgttctcg agtttattga agagcaaaac ctggcggcga actgtacgga
    12421 aaccggcgct tatctgcgtg cccggctgga ggagttgcgg gcaaaacatc cactcatcgg
    12481 cgaggttcgc ggaatgggtc tgctgcaggc gatagaactg gtccgcgacc gtgccagcaa
    12541 agagcctgcg gttcaggagg ccgctcgtgt tatggatgcc gcccgcgaac gtggagttct
    12601 gatcggcaag ggcggcctgt taggcaacgt ccttcgcatt tcgccgccca tgaacatcgg
    12661 caaagcggat gtggacgaat tcacgcgaat tctcgatgcc agcctgtcgg aagccggacg
    12721 gccttcatga gagcaaaccg cgcagcgcct tgacgccttc gacggcccgg tccagatgct
    12781 ccagagtgtt atagaggtgt gggcaaaggc gcaggcctcc cggtgtggcg gcggcgattc
    12841 caaactcctt gtaaagccgg tcgatgaccg tctgccggtt cgccgccggc acttcaacca
    12901 ccaggacgcc cgcgcttaac gacggatgcg acggcgtcgc cagcttcacg cccgcgccgg
    12961 cgaggcgttt ctttaacgtc gatgccagtt gcagcacgcg ggcttcgacg cgagaggggc
    13021 cgatcatgtc gtgaaaatcg gcggctgttc ccaatgcggc caggcaggcg tcatcccgtt
    13081 gtcccagcga ctcgaatttg cgcgctcctt ttacgtcggg ttcggcgtcg tcgccccacc
    13141 cgggagccac cgtgtgcggc cacacctccg gtatgcggct ggcccgtaca tagagcagcc
    13201 ctacttcttt gggtccgacg taccacttgt gtccgctcgc gctataggaa tcacatccca
    13261 tttcccgcag gttgacgtgc agcgcacccc acgtctgagc gccgtcgacg tgtacgtaga
    13321 taccgcgccg gtgcgccact tcacacagtt cgcggacggg caggcggagc ccgctgttgt
    13381 ttgacgtgtg cgtaatcgcg agcaggcggg tcttcggagt gaacgcgcgc tcaaaaacgc
    13441 ttaccaactc atcggcgtcc gcgggctgcc gtggcgtgct gactcgcttt atcgccaagt
    13501 gatatcgtgc cgccctcacg tcccaggcca cattgttggt ggggtggttc tggtcccaaa
    13561 gaacgatctc gtcaccggct ttgagcggta cgccggcgtt aatcgtatta ttcgcctcgc
    13621 tcgtgttacg gaccagggca atctcgtctg cggttacgcc cagctgccgg gcgactttcc
    13681 ctcgcgtctc ttccagcagg cggccgaact tcgcacggtt ctgggacgag cagtccatgt
    13741 cgatgtcgcg tgtcagttgg gtgacgcgct ccgccaccac gcggggagac gggcagaggt
    13801 tggcggcatt catcgggata tgagactcgt caaaatcgaa ctggcgccgt atggtctgcc
    13861 aatacgcttc gccctccgga gcagcggcca gcgcgcggtt caatccggag agagtggttg
    13921 cggcgcccgc ataaaacgcg gtgccggatg cgaaccgttc caggaatgtg cggcgggagt
    13981 gcccaggcat gaagcgattc ctccttgttg ccgctcatga tactaccggc agctcgcaag
    14041 ctacaacaaa gcccggtagc gaacagccag gggaaactgc aaaagtagct ggacagccgg
    14101 ccgtccgtgc atttatcatc catacaatac aaaccagtgc cgcgctctac cggtgccggg
    14161 agggagtcac atgggaaatt tgatcaagtc cagttcgctc attttcatcg tggcgttaat
    14221 tatctccgcc acgatccccg caacgttgtc cgcccaggtc ctatatgggt cggttacagg
    14281 aaccgtcacc gacggaaccg gggctgttgt accggctgcg aaggtcgtgc ttgttaatga
    14341 tgagaccggg ttgcgccgcg aagtagttac cgaccaggta ggcatctacc gcgtggttga
    14401 ccttccacaa ggcccttata cgctcgaggt ctcagccggc ggtttccagt cctataagaa
    14461 gaccaacatt cgcgtggtca ccggccaggt gaattcggag gacgtgccac tcgccgtagg
    14521 ctcgcctgcg caggagatca ccgtccaggg cacagccgct gtgctgcaga cgcaaaaagc
    14581 cgacgtccgc accgaaatca ccggacatgc cattcgcaat ctcccgttga acgtctatcg
    14641 caattaccag gcggtcacct tgctggcgcc cggcgtcttc tctacctcgg ccattgccga
    14701 cgcctatccg aacggtctcg cgagcggtcc cgagcgctcc atgagcatct actccaacgg
    14761 cctaccttcc cgcatcaaca acaatcgcgt tgacggcgcc accagcatta acatctttct
    14821 gccggatcac atgctcatca ttcccccggc cgagaccatt caggaagtga acgttcagac
    14881 cgcgaactac tccgttgaaa aaggcctcac ggcgggagcc gccaccgacg tcattaccaa
    14941 gtcgggcacc aatgaatttc acggctcgct ttatgctttc cacacgaaca acgcgctggt
    15001 cgcgcgcaac ttcttcgact tcagcccaaa gaaggccaag cgcatccaca acaacgacgg
    15061 tgttacgctg ggaggcccca tcctccgcaa caagctgttt ttcttcggca actgggacgg
    15121 ctacttcgag cgtaaagcca ccgccggcac cggattcctg gcccccatgg aataccggcg
    15181 gggagatttc cgcggcacgc tcggttcgcc gcttttcgac aaccagggcc agccggttac
    15241 ggtatgtacg accgaaggcg gctcgacaca actccggcag ggaatggtgt tcgaccctct
    15301 gacgggcgac cgcacaaacg gcaccggccg ctgcgtgttc tcgcacaacg gtcagatcaa
    15361 tgtccttccg cccaacagaa tcgttcaagg cgcgcagaac ttttggccgc gcctgaccgc
    15421 ccccagcatt cctggcacgg tcacgtcgaa cacgccgtac aatcacgcct acaaccagaa
    15481 caacagcttc aaccgcaaca tcggcatcgc caaggtggac tggaatcgta acgaccgcca
    15541 caccatctgg ggcaagtgga accttcaaga ctccctcgtc atcaccccgt tgacctttgg
    15601 cgacgcgggc gggccgtccg gcttcgatgg cgacattatc actcagatcg gcaccatcgg
    15661 gcacacctgg atgctctcgc cttccaccgt tctgaccggc catttcggac tcgcccgcca
    15721 gaagaccgat acgctcaacg gcctctcggg cgaaccgctc ggccagactg tcctcggcgt
    15781 tcccggcatg aacgaacctc tcgacgacgt ccgctacaca gggatgccgg cgatcggcat
    15841 ggccggttgg acggccctgg gcaacgccga tgggtccaca ccctacgttc ggcgagactg
    15901 gacctacacc atctcgcaaa acctcaccca catgcgcggg cggcacgagt tccgcatggg
    15961 catcgatctc ggagcgaact acctgaacca tttccaaccg gaagtgtgtt gcatccgcgg
    16021 cagaatggat ttcgcacagg gcatcacgtc cattaatctt cccgcgaacg cggcggcgcc
    16081 ggcgaccggc cagtacatgg aactgtacac ttcacctact cccagttccg caactcttgc
    16141 cggccgcggt tacagcgcgt tcctgcaaaa ctcggtggcg gcgttcaacc tcggcctgtt
    16201 cagccaggcg cagaagagca tccagtacat cgatttcacc gcccactcgt ggcaatacgg
    16261 cctttacttc ggagatcgct tcaaggctac ctcgcggttg acgttggacc tcggccttcg
    16321 ctgggagtat taccccatgg ttcgccgcag cggagccttc acatttgaca ggtatgaccc
    16381 cgacacgaac acgctgctgc tcggcggaat gggcggcaat cccgacgatc tcggtgtcac
    16441 caccagcaag aagctcttta cgccgcgcgt gggcatcgcc tacaggatcg gtgacaacac
    16501 ggccgtgcgc gcgggctacg gcattgcaat cgattccatg ccgatggagc ggccgatgcg
    16561 cggcttctat ccccaggtta tcggcgcgac gtacttcaac ccctccacgt tcgtgagtgg
    16621 cttcctgcct tatgccgact ttgccaccgg cataccggta cttgagggtc cggacatctc
    16681 cagcggcaag ctcatacctc caggcaatgt cacgatacac ttcgttccgc agggcgagtt
    16741 caaacgcggc tatgtgcagt cctggaactt ttccatcgag cggaagcttc cggctgagat
    16801 cctgctgaac gtcggctacg tcggcaacca cttcgtccat gaaatgaacg gccgcgacat
    16861 caacgccgct ccgctcggga ccggcagcgc cggccagcct ctcgcgaaat atggccggtt
    16921 tatcaccacc ccgtcgtatg agggctatct cgattcgaag tatcattcgc tccaggtctc
    16981 ggtgagccgc cgcgcggcgc gcgggctctt tttgcagggg tcctatacgt ggtcgaagac
    17041 actggcgtac tccgacgaca acacgtacgg gaacgcactg cgcttcaact gcccgccgtc
    17101 gcccgccatg cccgagggct gtctggaact gaactacggg cctaccagtt tcgatcgcac
    17161 gcagatggtc aaagcggcat tcgtatggga gctgcccttc gggtccggac acgctctgag
    17221 cagttccagc cgtgcggtga acgccattat cggcggctgg cagttgaacg gcatcttcac
    17281 ggccataacc ggagccccgc ttcagatcgg ccagagccgt aacggtttga acacccccgg
    17341 cactccgcag aatccctatg ttatgcgcca gcctgagtac atcaaagaac aggcgagctt
    17401 cgacgcctac agcggcatct actggttcaa ccccgacgcc tttgtgccga acttcacaaa
    17461 caacgagatc ggcaacattc cgcgccgtct ttcctggctg cgcggtcccg gcattactca
    17521 gttggacgcc agcattttcc gcaacttccg ctggaaggaa cggttcactt tggaggtgcg
    17581 tgcagaggcg atgaacgtga cgaactcgac gcactacagc gacccgaacg tcacctgcac
    17641 cgtcgtcgga aacgcgtgcc ggggatcctt tgggcagatc agctccgcgt tcgggcagcg
    17701 catcgcccaa cttggcgcgg tagtacgctt ctagcgatcc aatgcgacgg gccatcccag
    17761 ctctgctgct cctcgctacc gcggccggtg tcatgccggt cgcggcgcag gacgcctcta
    17821 ccgagcgccg caagcgcgac ctcgatcact tgctgaaggt cctgcttcca agcaggaccc
    17881 cggcgaccgg ccgcatcaat gcccacgaca aaacctggga ggagtggatc cggcgaaccg
    17941 gcgcgttacc gccggacttc gattcgatgc catcccactc cgagctcccc gatccgctgc
    18001 tcatgtacga gaacgggcgc gcccgcccgg tgaaaaccgc tgccgattgg gagcgccaga
    18061 agaagtggct gcgcgagcag gtctccaagt acatgttcgg ctacatgccg cccgcgcccg
    18121 gcaatgttcg cggtgtcatc accggaaccg aaaaggaagg cgacgtcacg atccgccatg
    18181 tgcgcctcga attcggcccg gagcaccgcg gtaccctgcg cgtgcagttg atgattccgc
    18241 ccggacaggg cccttttccc gttttcatga cgaaccatcc gcgcacgcgc ccctgggtgg
    18301 cgaccgcggt tcgaagaggc tatatcggct gtatctatta cgctgccgac cccatctacg
    18361 gcaacgacga cgattccgac aagttcatcg aactctatcc cgagtatgaa tttccggttc
    18421 tcgcacgctg ggcctgggcg ggcatgcgcg ctatcgacta cctgtacact ctgcccgagg
    18481 tggacaagga gaagatcgct ctttccggcc attcccgcaa cggcaagcag gcgctgcttg
    18541 ccgccgcgtt cgacgaacgc atcggcgcgg ttatcccttc gagcggcaac agcggcgaat
    18601 gcgacccctg gcgttacacc accgatatgt tcgccaacga aagcatccaa ctgcttgccg
    18661 gcgcgcagcc gcactggttc tccccgcacc tccgcttttt ttccggccgt gaagacaaac
    18721 ttcccgtcga ccagcacacg ctcatggccc tcgtcgcgcc tcgcggcctc atgccttaca
    18781 cagcgtattc ggagggggcg agcaatcccg tcggcttcga ggagggttat cgttccgcca
    18841 aacgcgtcta cgattttctg ggtgcaggca acaatatctg gctgcatctg cgcgccggtg
    18901 agcaccccac cgcggccggc gacatcgaga atttcatcga tttcaccgat acgatcttca
    18961 aacgtaagtc tttcccgaaa tttgagacct tcacgcacgg ctacaccttc gaaggatggc
    19021 gcaatctgac gggcgaaacc gtgaatccgt tgaagtaccc gaagcgtcag gtgggcgaga
    19081 cgctgaaatc cgtcaccagc gcgcaggcct ggcaggagaa gaaggagtcg ctgcggcgta
    19141 atctgctctg ggcgttgggt gaggaacccg ccggcatccg cttccctgct cgccgcgaac
    19201 tcggtggacg cgcccctatg acgaatccgg gttggctcgc ggggctgttc aatcgcccta
    19261 caccaaacgg ccccctggcc gcgcgcctca acaaagacgg cgtgggcgtg gccgacatcg
    19321 gcttcggcga tgatctgccc gctacactct tctatccatt gggcgaagac ggcaaaccga
    19381 aatccggaaa actgcccgtc gtgatctggc tacacgccta cccgtatcag catggctggt
    19441 cggcaagttc cccctggtca tcgacgggcg cgaattacgg cctggagcgg cgtcccagct
    19501 tccctgaact ggtgaagcgc gggttcgcag tgctcgcgtt cgatcagatc ggcttcggca
    19561 cgcgcgtgct cgattcggcc aggttttacg agcggtatcc gaagtggtca ttgatgggca
    19621 agatggtagc cgatacgcgc gccgccatcg aagcggtccg cgctctcgat atcatcgacc
    19681 cggatcgtgt gtatctgcta ggctatgcgc tcggagcgaa ggtcgggttg atgacggctg
    19741 cgctcgacgg cggtgtccgc ggggtcgcct cgatagccgg tgtggacgct ctgcggctca
    19801 gcacgccgga taagggcacc gaagggctcc gccactactc gcatcttcac gggacgcttc
    19861 ccaagctcgg gtacttcatc gggtacgagg accgggtccc gttcgattat gacgaagtgc
    19921 tggcgatggt cgcgcctaag cccacgctgg ttgttgctcc caccagggat tggtacgcac
    19981 gcatcgccga cgtgcagcga gagatccagg cggcagcccc ggttttccgc ctgctcgggc
    20041 gcgaactcga cgtgctgaca cccgacgact tcaaccgctt cccggtgcgg cttcaacagc
    20101 aggtctacga ctggttggcc aagctgccct gattcaacga tctgcggcat tgaggcatgg
    20161 cacgtccggt ttcagggccg gcgcagatcg gcccgtaaac gttgtttcaa ttgcgcaaaa
    20221 tgcgatatgc tccttggttg ggttcctgtg gtttcagtct tcctactccc ctagaggtct
    20281 cttatgtcag gacattcgaa atgggcgacg atcaagcata aaaaggccgc cctcgatgcc
    20341 aagcgtggaa aagtgttcac gcgcatcatt aaagaaataa tgattgcggc tcgcagcggc
    20401 ggcgatccg
//
LOCUS       DASTMK010000266         5460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_588791, whole genome shotgun sequence.
ACCESSION   DASTMK010000266 DASTMK010000000
VERSION     DASTMK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588791"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFQ79_11520"
     CDS             <1..181
                     /locus_tag="VFQ79_11520"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEU0140338.1"
                     /translation="AGFSGSVYQARLESANSAPAESAVRLVNVLRQFEMLQKAVTLGG
                     EMNRRSVEEVAKVNA"
     gene            202..993
                     /gene="flgG"
                     /locus_tag="VFQ79_11525"
     CDS             202..993
                     /gene="flgG"
                     /locus_tag="VFQ79_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEU0140339.1"
                     /translation="MIRALYSAGSGMQAQQMNVDNIAHNLANANTVGFKMRRTQFQDL
                     LYQSFVQPGAAAGSQTVVPSGLQLGLGTKPIANEIIFSQGNFSQTNNPLDLVIQGRGF
                     FQVRLPSGELAYSRAGNFHLDRDGNVVTANGDPLEPQITIPPEAQSITIAADGTVSYT
                     QPGQTASQLAGQIQLANFANPAGLNSLGGNLYAQTDASGEPTVGNPGGQEGIGSLLQG
                     YVEQSNVSVVEEFINMIVSQRAYEANSKVVRAADEMYQQVNNMAR"
     gene            990..1892
                     /gene="flgA"
                     /locus_tag="VFQ79_11530"
     CDS             990..1892
                     /gene="flgA"
                     /locus_tag="VFQ79_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEU0140340.1"
                     /translation="MKMFRATLLFISAVLTMAAGECIQVTGENIMVVELAPVHPAFGR
                     VPASEVFGHTPVPGGRRVIKGSDLARLAARYGFDGESFADTCVERRVAPLTREAVAAA
                     INASLNQPGAVVEIVDFSRFPVPSGKLDFPLGTLATPSPLNTGKPVLWRGTLTYDTRS
                     TLSVWARVRIRATQQRIVAAEDLKPGREIVAAQVRLVVVEDFPSKDPGLASVSAAVGQ
                     VPRRWIRAGEVLRPNLVTKPPEVKRGDRVQVQVESGSARIIVQALAESNGAAGEPVVV
                     KNLISGNRFRAKVQAKGKVLVSVN"
     gene            1903..2589
                     /locus_tag="VFQ79_11535"
     CDS             1903..2589
                     /locus_tag="VFQ79_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEU0140341.1"
                     /translation="MIKHLLPKTAAILFPVVLLLGKEPANKPAIETYVQESLARGASA
                     GTSPGSLYVTGGLLADVARDLRANQIGDLVTIVVADKASAIARGGTNTSRKSSAGYSV
                     GSIFGRTPAAGALANLAQANGENQLQGQGETSRETVLSATLTARVTHVLPNGNLVLEA
                     QKEVAVNSEKQMITVRGVVRPNDLGPSNLVRSDRLAFLEVAVNGKGVVGDAVRRPFIL
                     YRLLMGLLPL"
     gene            2606..3706
                     /locus_tag="VFQ79_11540"
     CDS             2606..3706
                     /locus_tag="VFQ79_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEU0140342.1"
                     /translation="MKIRVFFAIALLLGSSYLAASTRIKELAALEGVRENQLLGYGLV
                     VGLAGTGDKRQTVFSAQSLANMLERMGVSVPPTAMLVRNMAAVMITANLPSFAQPGAR
                     IDVSVAALGDASSLRGGLLLLTPLRAANGQVYAAAQGQVVVGGFVAGQGGTTQTLNHP
                     TSGRIPGGAIVEQRAPAAAPTGALKLQLNRPDFTTAATLAEVINKKLAPAGELIARAE
                     NAAIVTVNAPAAFTGRPVEFVAALENLPIETDSRAKIAVDEKTGTIVMGSDIRITPVS
                     ILHGSLTVEIQTNLAVSQPAPLSSGTTQVVPQVGVGVKEDKAQSVMLKQGATVQELVR
                     ALTAIGSTARDVIAILQTLRAAGALDAEIEVI"
     gene            3706..4008
                     /locus_tag="VFQ79_11545"
     CDS             3706..4008
                     /locus_tag="VFQ79_11545"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEU0140343.1"
                     /translation="MSDSISIPAMPFASSPAEASRSKRAEIQQAATQFESLLIQQMLK
                     SARESGASLSGESDSTGETVRDMADQQFAQVIAANGGVGLATLVLSGLQENDGGGR"
     gene            4027..4290
                     /locus_tag="VFQ79_11550"
     CDS             4027..4290
                     /locus_tag="VFQ79_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140344.1"
                     /translation="MRTNLLGILTMRVEAARPEPRSRSSASAQPVPDRPDTLRLSHIS
                     DVLSGQGTYSKPLALLADQFATGTYSVPAGQLSRLLVADTLSQ"
     gene            4416..4808
                     /locus_tag="VFQ79_11555"
     CDS             4416..4808
                     /locus_tag="VFQ79_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0140345.1"
                     /translation="MTLFGRWRKSAKAGNAFLQAKDLGVRIVRTASGTTVAVSGRVTI
                     DSSPHLRSLLLQLIRKETSQTVTIDLSKTSYLDTSGIATLLEALKAARRHPVKLRLVG
                     VSGQVKMLAEILELAQIFRAAGSEVVFS"
     gene            4832..>5460
                     /locus_tag="VFQ79_11560"
     CDS             4832..>5460
                     /locus_tag="VFQ79_11560"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0140346.1"
                     /translation="MPIRWFEELGALTIQFWAVLRKLPRVLPVVGRRRRWRASVQQMF
                     VIGTSALPMSGVMSLCTGFILALQSAAELRRFGALQFVVNLVAVGFTRELGPLITAIA
                     VSGRSASAISAEIGTMVVTEEVDALRVMGLDPVEFTLAPKYLAALITVPCLTILSTLC
                     GIFAGYVFLAFSIDMSLQIYFHAVAESILLRDIVLALVKSLVFATIIVQV"
BASE COUNT         1151 a         1580 c         1641 g         1088 t
ORIGIN      
        1 cgccgggttc tccggctcgg tgtaccaggc gcgcctggag agcgcaaatt cggcaccggc
       61 ggaatccgcg gtgcggcttg tcaacgtcct tcggcagttc gaaatgcttc agaaagcggt
      121 gacgctcggc ggtgaaatga accggcgtag cgtcgaggaa gtcgcgaaag tcaacgcctg
      181 agatggtgtg gaggtattgg aatgatcaga gcgctgtata gcgccggcag cggaatgcag
      241 gcgcaacaaa tgaacgtgga caatattgcg cacaaccttg cgaatgcaaa cacggtgggg
      301 ttcaaaatgc gcagaactca gtttcaggat cttctgtatc agagcttcgt gcaacccgga
      361 gctgctgctg gatcgcagac ggtggttcca agcgggctgc aactgggtct tggaacgaaa
      421 cccatcgcca acgaaattat cttctcgcag ggcaatttct cccagaccaa caatccgctc
      481 gatctggtga ttcaaggccg gggctttttt caagtgcgtc ttccgtccgg agaattggcc
      541 tattcccggg ccggcaactt tcatctcgac cgggatggca atgtcgtgac cgcgaacgga
      601 gatccgctcg agccgcaaat cacgattccg cccgaagcac aatccattac catcgccgct
      661 gacggaaccg tcagttacac gcagcctgga cagaccgctt cccagctcgc cgggcagatt
      721 caacttgcca acttcgcgaa ccccgccggt ttgaacagcc tcggcggaaa cctctacgcg
      781 cagaccgatg catccggtga accaacggtg ggtaaccccg gaggccagga gggcatcggc
      841 tctcttctgc aaggctatgt ggagcaatca aacgtcagcg tagtggaaga gttcatcaat
      901 atgatcgtga gccagcgagc ttacgaggcg aactcgaagg ttgtgcgcgc ggccgatgaa
      961 atgtaccagc aagtgaataa catggcgcga tgaaaatgtt ccgtgcaacg ctgcttttca
     1021 tatcggccgt tcttacgatg gcggcagggg agtgcattca ggttacgggt gaaaacatta
     1081 tggttgtcga acttgccccg gtgcatccag cattcggacg tgttccggca tcggaagtct
     1141 ttggtcatac acccgttcca ggaggccggc gcgttatcaa aggttccgac ctcgccaggc
     1201 tcgcagcacg ctatggattc gacggagagt cgtttgccga tacttgtgtg gagaggcgcg
     1261 tggccccgtt aacgcgcgag gccgtggccg cggcgatcaa cgccagcctg aaccaacccg
     1321 gagcggtcgt agagatcgtt gacttcagcc ggttcccggt gccctcggga aaactcgatt
     1381 ttccacttgg tacgcttgcg acaccatcac cactgaacac cggcaaaccc gtactttggc
     1441 gagggacgtt gacctatgac acccggagta ccctgtccgt ttgggctcgc gtgcgaatcc
     1501 gcgccacgca gcaaaggatc gtagctgccg aagaccttaa gccgggtcgg gaaatcgtcg
     1561 cggcgcaagt ccggctggtt gtagtagagg actttccgtc aaaagatcca ggactcgctt
     1621 ccgtcagcgc ggccgtgggg caggttcctc gaaggtggat acgcgcggga gaagtgctcc
     1681 ggccgaacct ggtgacgaag ccgcctgaag taaagcgggg cgaccgggtg caagtccaag
     1741 tcgagagcgg cagcgcgcgc attatcgttc aagcccttgc ggagtcgaat ggagcggccg
     1801 gcgaaccggt agttgtcaag aatctgatta gcggcaatcg cttccgggcc aaagtgcaag
     1861 ccaaaggaaa agtgcttgtc tcagtgaact agagaatcga acatgatcaa acatctcttg
     1921 ccaaaaacgg ccgcgatcct ctttccggtg gtactccttc tgggaaagga accagccaat
     1981 aaaccggcca tcgagaccta tgttcaggaa tcgcttgccc gcggagccag cgcaggcacc
     2041 tctccgggtt cgctctacgt gacgggcgga ctgttggccg acgtggcacg ggacctcaga
     2101 gcaaaccaga tcggggacct tgtaaccatc gtcgtcgctg acaaagcgtc ggcaatcgcg
     2161 cgcggcggca cgaatacctc gcggaaatcc agtgccgggt attctgtcgg cagtattttc
     2221 ggcaggaccc ctgccgccgg tgccctggcg aacctcgccc aggcgaacgg tgaaaaccag
     2281 cttcagggac agggagagac cagccgcgaa acggtgctgt cggcgacgct gacagcgcgc
     2341 gtcacacatg tgttacccaa cggcaatctg gtgctggagg cgcagaaaga agtggcggtg
     2401 aactccgaaa agcagatgat cacagtgcgt ggtgtcgtgc ggccgaatga ccttggtcca
     2461 tcgaatctgg tccgctcgga ccggctcgca tttctcgaag tggctgtcaa cggaaaaggg
     2521 gtggttggcg acgccgtgcg gcgtcccttc attctctacc ggctgctaat gggactactt
     2581 ccgctctgat cggaataggg tttagatgaa gatccgagtt ttctttgcaa ttgcccttct
     2641 gctggggtca agttatctgg cagcctccac ccgcatcaag gaactggcgg cgttggaagg
     2701 tgtgcgagaa aaccaactcc tcggctacgg tcttgtggtc ggcctggcag gcacaggcga
     2761 taagcgccag acggtgttct cggcacagag tcttgcgaat atgctggaac gcatgggggt
     2821 gtccgtgccg cccaccgcaa tgctggtgcg aaacatggcg gcggtaatga tcacggccaa
     2881 tctcccgtcg ttcgctcaac cgggagcgcg catcgatgtc tcggtggcgg ctctcggaga
     2941 cgcgtccagc ctgcgcggcg ggctgctcct gctgacacct ctacgggcgg caaacggaca
     3001 ggtctatgca gccgcacaag gacaagtagt ggttggggga tttgtcgcgg ggcagggggg
     3061 caccacgcag acgctaaatc atccgacgtc gggccggatc ccgggcggcg ccatcgtcga
     3121 acaacgagcg cctgcggcgg ccccaacggg tgcattgaag ctgcaattga acaggcccga
     3181 cttcaccacc gccgccacgc tagccgaagt catcaacaaa aagcttgcgc cggccggaga
     3241 acttattgcg agagctgaga atgccgcaat cgttaccgtc aacgcgcctg cggcgtttac
     3301 cggccgcccg gtggagtttg tcgccgccct ggagaatctt cccattgaaa cggattcgcg
     3361 tgcgaagatc gctgtcgacg aaaaaacggg aacgatcgtc atgggaagcg acatccgcat
     3421 caccccggtc agcatcctgc acggcagcct caccgttgaa atccaaacca acctggccgt
     3481 ctcccagccc gcaccgctgt cctccgggac gactcaggtc gtcccgcaag tcggtgtagg
     3541 tgtaaaggaa gacaaagcac aaagcgtgat gctcaaacaa ggagcaacgg ttcaagaact
     3601 ggtgcgcgcg cttaccgcaa ttggctccac cgcacgcgac gtcatcgcca ttttgcagac
     3661 gctacgcgcc gcgggagcgc tcgatgcaga aatcgaggtg atttaatgag cgattccata
     3721 tcgattccgg ccatgccatt cgcttccagt ccggcagagg cctcccgctc gaaacgggct
     3781 gaaatccagc aagccgctac gcagttcgaa tcactgctca tccagcagat gctgaagtcg
     3841 gcgagggaat ccggagccag cctatccgga gagtccgaca gcaccggcga gacggtccgg
     3901 gatatggcgg atcagcagtt cgcccaggtt atcgctgcga acggcggtgt cggacttgcc
     3961 acactcgttt tgtcggggct gcaagaaaac gacggcggcg gccgataaga acagcagaga
     4021 cattagatgc gtacgaacct tctcggcatc ctcacgatgc gtgtcgaagc cgcgcggcct
     4081 gagccgcggt cacgatcgtc tgcttccgcg cagccggtgc cggaccggcc ggacactcta
     4141 cgtctctctc atatttccga tgttctcagc ggccagggga catattcgaa gcctctcgcc
     4201 ctgctggccg atcaatttgc caccggaact tattcggtac cggccggcca gcttagccgg
     4261 cttctggtgg ccgataccct gtcgcaatag catccacaca cctcacatcg ttggcttggc
     4321 ccggtgccgg gtcctgataa agtttagaaa ggcccgcacg gcgaagagat tgtatcaccc
     4381 cgtgaggccc gggaagctcc acgtttattt gctgaatgac actatttggt cgttggagaa
     4441 aaagcgccaa agccggtaat gcgtttcttc aggcgaagga tctcggcgtg cgaattgtcc
     4501 ggacggcttc cgggacgact gttgccgtaa gcgggcgcgt gaccatcgat tcgtcaccgc
     4561 atctgcgctc gctgcttctt caactgattc gcaaagaaac cagtcaaact gtcaccatcg
     4621 acctttcaaa aacctcctat ctggacacct caggcattgc aacactgctg gaggccctga
     4681 aggcagcccg gcggcaccct gtgaagttgc gcctggttgg agtaagcggc caggtcaaaa
     4741 tgctggcgga aatcctggaa ctcgcccaaa ttttccgggc tgccggttcg gaggtggttt
     4801 tcagttgact gttctggaac gagtggggca agtgccaatc cgttggttcg aggaacttgg
     4861 tgcattaacc atacagttct gggcggtgct ccggaaactc ccgcgcgttt tgccggttgt
     4921 cggaagaaga aggcgctggc gcgcctccgt gcagcagatg tttgtgatag ggacaagcgc
     4981 acttcccatg tcgggcgtca tgtcgctgtg caccggcttc atccttgcac ttcagagcgc
     5041 cgccgagttg cgccgtttcg gcgccctgca attcgtagtt aacctggtgg cggtgggttt
     5101 cacaagagaa cttggccccc tcattaccgc catcgcagtg agcggccggt ccgcatccgc
     5161 gatatcggct gaaatcggaa ccatggtcgt caccgaggag gtggacgcgc tgcgggtgat
     5221 gggattggac cctgtggagt tcacccttgc gccaaagtat ctggccgctc tgatcacggt
     5281 tccatgtctg acgatcctga gcaccctgtg cggaattttc gccggctacg tgtttcttgc
     5341 cttcagcatc gatatgagtc ttcagattta ctttcacgcg gtcgccgagt cgatactgtt
     5401 acgcgacatc gttctagctc ttgtgaagag tctcgtcttc gctaccatca tcgtccaggt
//
LOCUS       DASTMK010000267        31254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_76313, whole genome shotgun sequence.
ACCESSION   DASTMK010000267 DASTMK010000000
VERSION     DASTMK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 31254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31254
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76313"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1750
                     /locus_tag="VFQ79_11565"
     CDS             215..1750
                     /locus_tag="VFQ79_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140347.1"
                     /translation="MNRREFVSSSLLVAGAAGSHAQIRTGFQAGYAERDITPAIGMEQ
                     PGGYGKSYHTKLHDPCKVRTAVFDGGATKVALVGVDAGSVGRRMVLAAREGIRNRCGI
                     PADAVLIGASHSHSSGPIGGVEPGEYDHASALVRELAYEKSTVNNPKYVEHVQNQIVD
                     AVCAAAGSLRPMASGVGSGIEDKVAFNRRFRMKNGLSYTHPGQGNPDIVKPAGPIDPT
                     VGVIGSWDDSGKLAGCIVNYSCHATTNPGGISANWIYYMERTIRGFFGPDVVVVFLQG
                     ASGDVTQVDNLSPYRRASGAREAQFVGGRVGAEAVKVLLTMHPGELTPVTSASRLLRI
                     KRRTPSAEKVKRAYQIVGQDPKEAGMTAWTFAKETVMLDAKLAKEPVADVEVQAIQVG
                     PAVFLSNPAELFVEFGLEQRRRSRFPFTFPVELANGDAGYVPTEEALGPHGGGYETRL
                     TSYSNLVPTAGRQMTEAGLELADRMQPGKVPEPPRAEPFKSREGGIGSEAWSYGNVPP
                     ELS"
     gene            complement(1760..1939)
                     /locus_tag="VFQ79_11570"
     CDS             complement(1760..1939)
                     /locus_tag="VFQ79_11570"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEU0140348.1"
                     /translation="MSFGRRVAARSSIASELLLFFWRGKWWWITPIVVAMLVFAVFIV
                     LAQSSPLAPFIYTLF"
     gene            complement(1952..3688)
                     /locus_tag="VFQ79_11575"
     CDS             complement(1952..3688)
                     /locus_tag="VFQ79_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEU0140349.1"
                     /translation="MRILGVSCYFHDAAAALLLDGQLVAAAEEERFSRIKHDPSFPQR
                     AIEFCLNTAGLEGKDLDYVVFFEKPFLKFERLLVSCLQGFPRSRRLFQEAMISWLGDK
                     LWIKDLLARRLGLPHSRVLFSRHHLSHAASAFFCSPYEEAAILTIDGVGEWATAAIGV
                     GRSTDIRLLEEIRFPHSLGLLYSAFTAFLGFEVNEGEYKVMGMAPYGEPKYLDEVYRL
                     IRVNADGSFELNLDYFSFQHSLEATFNTRFEHLFGNPRNPAQRFDPREKESRRYADVA
                     ASIQAVTEDIVVRMAAHAQRLTGMRRLCLAGGVALNSAANGRLLREGLFDEIYIQPAA
                     GDSGGALGAALYAHHMVLGQPRAFVMEHAAWGEEHSPDTIRKFLDGSGLHYRCFRDEG
                     LLIQEATGQLARGLVIGWCQGRFEWGPRALGNRSILADPSGAQMKDTVNQKIKFREEF
                     RPFAPAVSLPRAPEYFDLPGPERQYPARFMLYVVPVRAEKRDAIPAVTHVDGTARVQV
                     VPSESGSLYSRLIGAFGEATGTPVLLNTSFNLKGEPIVNTPEEACASWKRSGMDTLVL
                     GPFLVEKRDQSG"
     gene            complement(3692..3898)
                     /locus_tag="VFQ79_11580"
     CDS             complement(3692..3898)
                     /locus_tag="VFQ79_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140350.1"
                     /translation="MLRRMWNSWKRVARRIADFQIRMLLALFYYTLLAPFALLVRVRE
                     AHSAGWTAPAEPSISEQQRAQEQS"
     gene            complement(3905..6154)
                     /locus_tag="VFQ79_11585"
     CDS             complement(3905..6154)
                     /locus_tag="VFQ79_11585"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140351.1"
                     /translation="MRGWIPRIALIFLAPVAAFALFEAVLRISDYGFATQFFVPAAQS
                     GEYTTNQRFGERFFPKALARTPVPALLNKQKSPGTYRIFVLGESAAMGVPEPAYSFGR
                     VLEVMLREMYPAAKFEVVNAAMTAINSHAILPIARDCASFQPDLFVALIGNNEVVGPF
                     GPGTVFGTMPPALWFARVNVWQTSTRTGQLFRNVLARFGRQPETVEWRGMEMFLDKRV
                     ARDAGPLHAAYDNFRRNLEDIAQTAERAGAAILFSTVPSNLKDSPPFASAQPDNERWQ
                     GLYRRGVELEMAEQWSKALAAYKQAAALDEESAELHFRIGRCYLALNQTGAARDAFIA
                     ARDRDLLRFRTDSVLNGIIRKLRGARVYIVDAEAGFGLAGHERFYEHVHLNEQGNYEL
                     ARLIAQRFDSIAPRWIERTGSMPFMEAVAAELALTDWDRYKLARQMHAMMDRPPFTNQ
                     VGSAQARSKREEALHRVEENAGAAGAMDHARHIYENALAERPADLALRVRWAEFLRDE
                     RQYARAAEEWRILNSRLPGIANWYAAHAAALADAGRIDEAISQYREALRIDPDSDVAL
                     FGLGSAFAAKGNYQEAVLRYGEALRINPGYAEVHNNLGLIHLARGDLDRAQASFASAV
                     KYQPEFADAHYNLGGVLMQQGKTEEAAREYGIAVSQRPQFAEAHYNLGLLASRAGRLK
                     EALVHYADAIRLKPDYPEAHNNMGTAFAREGDMKRAVHHFSEAIRLRPDYAPARQNLS
                     KALQSARRR"
     gene            complement(6164..7609)
                     /locus_tag="VFQ79_11590"
     CDS             complement(6164..7609)
                     /locus_tag="VFQ79_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140352.1"
                     /translation="MKRLVLFGAAVLMTAAGLRAQPAGERVFRAGAATTNITPPLGSP
                     IVGGWNSPPATNVHDELHSRALVLDDGSTRLAIVLCDSVGITRVVFDEAKRMVQEKVG
                     LPPENILMAATHTHSASSARGGRGITPAPLDEYQTFLAYRIRDAVLMAIYRLEPARIG
                     WGVAFNDRQVFNRRWKMKPGSKHLRNPFGGIDKVRMNPPANDPDLIEPAGPTDPQINF
                     ISVQSTAGKPIALLANYSLHYVGGVKGGDISGDYFAVFAERIAELLNARNQNPPFVGI
                     LSNGTSGNINNINFRPGPDAARRRFEPYEKMRMVADEIATGVFEAYRKVEHRDWVPLA
                     AAQKDLMLGVRVPDAEQVEWAQRTLKDPPKYHQHEQVYAERLLRMKDWPREAPVPLQT
                     FRIGDAGIAAVPAEVFVEIGLEIRKRSPLKQTFTIELANGWFGYLPTAEQHEVGGYET
                     WRGTNLLETRAAPKIVEGIVDLFQKVAAPRN"
     gene            7705..9714
                     /locus_tag="VFQ79_11595"
     CDS             7705..9714
                     /locus_tag="VFQ79_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140353.1"
                     /translation="MRRLGVWFCALFCTTGVFSGQPAYTDAAECAACHPAIAKTYERT
                     GMGRSFYRAAEDYVPDGSYYHEASDRYYAVVRRGGEPFLRRWQREANGPEFNIEEKRI
                     DYVLGSGNHARSYVSRAPDGRLLALPLAWYAEDGGKWAMAPGYDRPDQADFRREIGFD
                     CMFCHNGYPEMESGAARFGKPARFRGRIPEGIDCQRCHGPGSAHVEAARSGAGQDRVR
                     AEIVNPARLSHARQLEVCLQCHLESTSRRLPYAVRRYHRDIFSYRPGEPLGDYMVHLD
                     LAPGSGRAETFEVVNAGYQFLKSACYRKSGEQLTCTTCHNPHDVDRGDAARTRIASIC
                     QGCHENVRHRTPVTYDCTGCHMPKQRTDDAVHVVMTEHLIRRTPLPEDLLAPKREVHE
                     TGEHTHLGKVVPLYPKKLSTLDELYLAVAQVRESANLREGIPRLQNALEKFRPKEAPF
                     YFELGEALRNVGRHRDAVRYYEQAIERDPDFLSAARGLGSALHAAGDYNRAIAVLEKA
                     AESDSESVETWNVLGSAYDAGGRPEKAMAAWRRAMEVNPDTPEPHLNLGVALSRSGNR
                     QASAAAFLEAIRLRPDFAAAHNNLAVVLEQQGEDIRAARHFERAIHLDAAYAGARYNY
                     GVYLARRKRWAEARSQLEEALRLQPAAEVRTALEMVKKLEDQFTR"
     gene            9783..10754
                     /locus_tag="VFQ79_11600"
     CDS             9783..10754
                     /locus_tag="VFQ79_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140354.1"
                     /translation="MGTALRILLMLLTVAGFSLASESIRICYPDGVFRFGSPVSTADK
                     GFILSRAFESVDGSAPNVWVYDREGKAVTSAILWLEDAAKVRVFDVGVDYDGTAVASG
                     YVTRADGTAEAVIGIIGRGGRIERVIRTNPFVARFVTVGPDRVIWAAGRDIEATQRGR
                     DYDMVRSYSMDGRFLHSAVPRSSAASVQDPVPATGKTGRTHLRGSSTNVVFYSGVMNR
                     LIVFDRAGAIVNDWSPPVPWRNARMGGFGTDAASIYALFRGDDGHRGAFRFDRQAGNW
                     VLIKDTYTPPGERVRFGLLRGTDAGALVFTNRKMPESCVTRLPVPGP"
     gene            complement(10762..11868)
                     /locus_tag="VFQ79_11605"
     CDS             complement(10762..11868)
                     /locus_tag="VFQ79_11605"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0140355.1"
                     /translation="MPDKLVLEEFQPKSMLVVPEHPVNRARFPVIDVHTHISSIFRSR
                     RSAPPIDRETALKQLDEIAKWTKEINLVRIINVTGGWGDDLKRNVNDLQERYNSHFVT
                     CTTPAWDRIREQDYPKWQADELANAKKSGAVGLKISKTLGLYLRENGREGPLVKIDDP
                     RFDPMWEQAGALKMPVFIHVADPDAFFTPTDRFNERWEELGNHPDWSFYGKDFPPKPE
                     LLAARNRIVARHPKTQFIGLHVANRPENLDEVSAWLKQYPNLHCEVGARLGEIGRQPR
                     RARKFFEQFQDRIMFGTDGTPNGKEVPQQDLVPAMYQAYFRFLETLDEYFDYAPSATP
                     PQGRWRIYGIGLPDAILKKVYHNNAARLLGWKTV"
     gene            12055..12423
                     /locus_tag="VFQ79_11610"
     CDS             12055..12423
                     /locus_tag="VFQ79_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU0140356.1"
                     /translation="MIFNPPRCYTSTKIYSEFESKELDMPVHELGIYVFILAAFLGLE
                     LIRRVSPLLHTPLMSLTNAISAISVVGAIVIAGEENASTFSRVLGFIAVTTATINVVS
                     GYLITDRMLKMFKKQERGKP"
     gene            12423..13793
                     /locus_tag="VFQ79_11615"
     CDS             12423..13793
                     /locus_tag="VFQ79_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU0140357.1"
                     /translation="MSLIVSFSYIAAAVLFILALKWMSAPASARRGVRAGELGMLLAV
                     VGTLLRNEVVDYEWIAAGLLLGSAIGVPLAYLMPMTAVPQRTAISHAFGALAAALIGT
                     AEFYKHPQHGFILGALVIEVLLGYLTFTGSLMAFGKLQEILPGRPLVYKGQNIVNLTV
                     FAAAVLAGIYLIANPSATWAFPVLSVLALLFGIMLIVPIGGADMPTVIALLNSYAGLA
                     ASFMGFALDNYLLVIAGALDGASGLILSIIMCRAMNRSFANVLFGAFGQVQAKAGAVE
                     KRPVRSAEPEEAAEILEAARLVIIVPGYGMAVAQAQHKIRELYDRLTKLGVEVKFAIH
                     PVAGRMPGHMNVLLAEADIPYDRLSELDEINPEFAQADVVLVVGANDVVNPAARTDSS
                     SPIYGMPILDADKARTVMVIKRSMNPGFAGIENELYYMNKTLMLFGDAKAVLGEMNKS
                     LAAQRV"
     gene            13853..14437
                     /locus_tag="VFQ79_11620"
     CDS             13853..14437
                     /locus_tag="VFQ79_11620"
                     /inference="COORDINATES: protein motif:HMM:NF039786.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEU0140358.1"
                     /translation="MHRTPIHFLALAVLAAPLAFAHCDGLDGPVVAAGREALRSADVN
                     LVMPWVQPGDEPAIRQAFARALKVRKLNPDAAELADTWFFETLVRIHRAGEGAPYDGL
                     KPAGRDLGPAIPASDKAIETGDADALEKLLSAPAREQLHKRFRRVVDARNYSSVDVKA
                     GREYVAAYVDFVHYVERLYEAITAGSGHAATHEH"
     gene            complement(14450..14872)
                     /locus_tag="VFQ79_11625"
     CDS             complement(14450..14872)
                     /locus_tag="VFQ79_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140359.1"
                     /translation="MAQGGGRALLRWTWILVVLALLYTASVFVLRWRHNRAAVEAIER
                     SRTEADRKIVEQYGASELKILTFYASPGVLKRGDTGLLCYGVLNAKSVHIEPGVEEVH
                     PALSRCVEVKPDATTVYTLSAVDDSDKAVSQTVEVRVQ"
     gene            complement(14901..16124)
                     /locus_tag="VFQ79_11630"
     CDS             complement(14901..16124)
                     /locus_tag="VFQ79_11630"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0140360.1"
                     /translation="MIRRAFLASGAAAAVVQSLSGQSGQRPQRRYDILIRNGEVRDPG
                     RNYRLQADVAILDGKIAAIEQNISADRALDVIDAKGLYVTPGLVDLHTHCFYGGGGVS
                     IEADPISARSGTTTWVDAGSFAYDQVIGFRRFAVDASRTRIFGYMHLYPHLTNPDVDV
                     VKYVRSGVRRTGEAALANRDIVLGIKVFVGSNMNGRYSYDFLKIARELGDEFKLPLMA
                     HISLAPPEIHQVLELLRAGDVVTHCNNGHTLGIVDSGYSAKLGQLKPGVAEARERGVL
                     FDVGHGAGSFNFNAARTAIHAGFLPDCISTDLHSACVNGPTYDLPTTMTKYLHLGLSF
                     DDVLLRTTANPAKVVGRLPGMGSLTVGGPADVALLEMQKGDFRLVDSQRNVVQVNEKV
                     VSRLTICRGKRLTAV"
     gene            16497..17327
                     /locus_tag="VFQ79_11635"
     CDS             16497..17327
                     /locus_tag="VFQ79_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0140361.1"
                     /translation="MKEFRKSKPIRVLAKTFSIIEAVQDSPAPLTLKAISEQTAVNKS
                     TASRILAHLETQRYVTRDAKGGYRVGEKLLQLGTRPASEVNLREAAQAPLRELWRITQ
                     ETVNLGILDGLEVMYLDGLESPQSFRLVSSAGTRAIAYRTALGKAILAYLPAEEREAV
                     ITSFAFQPYTPHTIASAEQLRNELEHVRAKGYAIDDEESVIGVRCLAAPVLSPARTAT
                     AAVSISGPVARMADDKIEEFSRAARAAAREITERLRTIGQSRLPDRRFVQQRQCNHSA
                     "
     gene            17393..17707
                     /locus_tag="VFQ79_11640"
     CDS             17393..17707
                     /locus_tag="VFQ79_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0140362.1"
                     /translation="MARKPQSGPASDPEILILSSLAGGPKHGYAILADIDSFIGGRMG
                     PGTLYTAITRLVDKGWIAPESPAGRQRPYRLTAEGAACLEELLKNMRRVAKIGLERLR
                     LA"
     gene            17704..18372
                     /locus_tag="VFQ79_11645"
     CDS             17704..18372
                     /locus_tag="VFQ79_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140363.1"
                     /translation="MNSLKAQIVAALAGLYPRRWKREYGSEFADVLIRSPLGPSAVLN
                     IAWNALRQQLRLGEPWLIVGIPWLLLNLYGHLWNILYPVPYAADPSSNSLAARAVAGL
                     LPLAIGYWTVLRDQINGHGGRAAMKYFLLIQWPICALAILWGLGILRIIVLGPGDPPS
                     TFHEHGFAYTFYDYPRRPIHWQLLFWKPFGDLPFMGVLGWLGGLAARAQARFPGRRRS
                     PGNV"
     gene            complement(18380..19447)
                     /locus_tag="VFQ79_11650"
     CDS             complement(18380..19447)
                     /locus_tag="VFQ79_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140364.1"
                     /translation="MRDSRVGFGLLGAGLIAPFHARALQASKVAELVAAADVDAARLA
                     RITDTFGCKGYSSLEAMLEDPTLDVVSVITPNHLHADAVLKAAAAGKHVLIEKPPAMS
                     LREVDEMAAACDKAGVKACVTVQCRTRKAVQVIRDALAAGRFGKIYHADAYMKWFRST
                     EYYRSDAWRMSRRSGAGVTIQQAFHYVDLLQYLVGPAKTVEARMTNLAHPDIDVEDTL
                     LAFVKYENGAQGVVQASTALWPGTDVRIEINGENGTAIMVGERVDTWKFREERPEDEE
                     IRTYGSAAVATGATGAADLGFQDHQVVIDDMACAILNGREPMIGLTSVRPTLEWSLAM
                     YLSAKSGAAVTLPLLDESAVW"
     gene            complement(19461..20498)
                     /locus_tag="VFQ79_11655"
     CDS             complement(19461..20498)
                     /locus_tag="VFQ79_11655"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0140365.1"
                     /translation="MKAIVKTQKGPGFVELMDMPEPEIGAGEVLIEVKACGICGTDVH
                     VLHDEFPYWPPVILGHEFAGQVIETGPGTTLFQKGDRVVGEPHTQACGQCYLCRTGNI
                     QICGMKRSPGWGIHGAFTRYLKMPERLLHRIPDNVSYEAAAVVEPAANTVHDIIERAK
                     IEAGDFVVVVGPGPIGLLAAMTARAGGSRRTIVIGAKQDEAVRLAKARELGFDTLVAG
                     GTIDPMEAVLEMTGGIGADLVVECSGSAAGIASAIDLVRKKGRICAIGLTGKQTIPFP
                     WDKAAFKVCDLIFNLSTSYTSWDRTINLAAAGMLPVEKVISHRLPLSKWDDAIREIEA
                     QTALKVLLTPE"
     gene            complement(20495..21751)
                     /locus_tag="VFQ79_11660"
     CDS             complement(20495..21751)
                     /locus_tag="VFQ79_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0140366.1"
                     /translation="MTTDISLSELREAVASVRDDAQQFLCELIRVPSLPGKEADAIAC
                     AEKWFAGKAEVERVPLSNSLRQDEDYADPVPGIEYDGRWNLRVRVPGSGGGRSLLFNT
                     HLDVVPASEGQNRPFDPVVENGVVRGRGACDAKGQAAAIFTAIAALHRLGVRLNGDLL
                     AHLVVEEEVGGNGTLAMIRKGEQADACIVMEPTDQRILSSVRGAVWFRVICTGKPGHS
                     GSAGSTVSALKMAVRVMEILEGYHARLLASSRGIAMFDDYPNPMPITFGKLNAGNWPA
                     SAPAEAVIEGVLGLLPNKTRYEVMEEMRQAIASEADEWLREHFKLEFMYRHDAHVLDP
                     SHPLAAGLSAACREAGLEGGSGAMTASCDSWFYNNQLKIPSVVFGPGSLSVAHSREEH
                     IRMDDICNAAVALLNFAQNWCGRSES"
     gene            complement(21764..23092)
                     /locus_tag="VFQ79_11665"
     CDS             complement(21764..23092)
                     /locus_tag="VFQ79_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0140367.1"
                     /translation="MGTETTAAARVHCQLPGPRSRELFERWAKVEAQCTGYQARVCWD
                     HANGVVVTDVDGNTFIDWTSGVLVTNVGHSHPRLVEAVQTACAKLMNNYECLNEPRVN
                     AAEKLVQILPAHLDKCFFLSTGSEATEACIRLMKRKSGKFEIISFHGGFHGRTYAALG
                     AGGLAGPKKGYGPTLPGSIRVPFPYCYRCPFRAAPETCDMLCLDYLDDAVRANSTGSL
                     AGVIVEAYLGAAGFIFAPPGWFPRLEQWIRDRGLLFTLDEVQSSFGRTGKLFAMEWEN
                     LTPDLVALGKGIGSGVPASAVAARGEVIGVLGPGEMSSTMGGNPVACAAVVAVVDIME
                     SEKLHENALRMGEVMKARLLQIQEKSPYVGDVRGRGLVMGVELVKDKKTKEPAPDLTR
                     KLIDVAAQNGLLIGSVGVFGNVIRVAPPLVISEAEAHESLDIFEKSLAQL"
     gene            complement(23095..24102)
                     /locus_tag="VFQ79_11670"
     CDS             complement(23095..24102)
                     /locus_tag="VFQ79_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEU0140368.1"
                     /translation="MKVVVTDYIEPDLNWEAEKLGGQGIELAPYQLKFAPFEDVAAAT
                     RDADVVVVNMVPITRELIESWTRCRLVIRHGVGYDNVDRAALDEAGIPLCYIPDYCVE
                     EVAEQAIALIFACARRIPRSRTVLDESSQNGRWDFKDVIPMFRMAGQKLGILGCGRIG
                     SRVYQKLKSFGFEFVICDPYLSEKRKRELDIELVDKETLFRESDFITIHTPLAPETRY
                     IVNRETLALMKPTAYLVNTARGGMVDVDALAEALKNGVIAGAGIDVYETEPPRPDYPL
                     FGLPNAILTPHLAWYSEDAGWKIREIIVLEIERFAAGLAPGYVANNVSAPRMPVETGR
                     S"
     gene            complement(24099..25190)
                     /locus_tag="VFQ79_11675"
     CDS             complement(24099..25190)
                     /locus_tag="VFQ79_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140369.1"
                     /translation="MSGLSVGFIGLSHLHPKSYMPVFGAVAGMKVVAAADGNPAVLES
                     FTRDFPVHGYADWREMLDRERLDLAVLFLPHNLCPEAAIACAEKGVHVLAEKPMAAGS
                     DGLRCMMAAAAKAGVILSTPYVWRYHPVARAMKQYVDDGVLGRIVGGEGRCAAGRLHR
                     YIEGNAAWMLNRRMSGGGPMYNLGVHWIDLYRWMLDDEVVEVLGKNVKINQEYDIEDN
                     SFALLTFSRGTVLALDISYTVPDSFPAGRDLYLALRGTAGVLSWSPSFEGIRENLFVC
                     SDAPGFAPATRRRIDFELDPQPGYAGPLTLPFLKELAQSIREGKPPAITGEDGLRALE
                     VVEAIYESAGSGRAVSLAGALATAEKDER"
     gene            25387..26421
                     /locus_tag="VFQ79_11680"
     CDS             25387..26421
                     /locus_tag="VFQ79_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0140370.1"
                     /translation="MKHDDQANDPRTASGRRQMLARSAAFLAAPGMLAAGSTQPAPAP
                     QKPVRPRSTPRRYEMKKSINLWAFPYPGQWSLKDCLQISKDAGFDAVELNFDLTGEFS
                     AESSDARIKEIGELARKVGIGISGICSFLFWPYPLTANDPEKRKKAMALGARMIEAAS
                     LLGTENLLVVPGAVYASWVEGFEPVPNDVCDRRAREAIRAMIPLAEKAGVYLNLENIF
                     SSGFLFSPQEMNEFVDSFRSNRVRIHFDTGNIMQYQFPEHWVPILGKRIKNIHFKEWD
                     KRTHEFNLHTFRPLLDGTTNWPAVLVELDKIGYRDYLTFEYFHPFQHYPEALIYQTSD
                     ALDWMLGRKG"
     gene            complement(26443..27477)
                     /locus_tag="VFQ79_11685"
     CDS             complement(26443..27477)
                     /locus_tag="VFQ79_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0140371.1"
                     /translation="MRRRSFLAAASAAAVPPVPAAPNVKRGVDLFSLRSQGWTAFEYL
                     AYCAKLNAKVVHFSEIRFLGGLDPSHLRKVRARAEELGIETEIGMRSICPTSKLFDPS
                     QGTAEQQLERMIEAAAIIGSPIVRAFLGSMADRTGSIPIEGHIENTAKVLRSVKSRAE
                     SKGIKIAVENHAGDMQGRELKMLIEEAGKDFTGACIDSGNPLWTIEDPHVTLDHLHPY
                     VLTSHVRDSAVWRVPEGAAVQWVRMGEGNVKIDEYVRKYLALCPGKALSLEIIVTQPR
                     IFAFAKPEFWDGYRRMPAWEFARFLQLADAGTPRQPTPAAGKDAAAAREREDLEASAR
                     YMERLLGAAA"
     gene            complement(27499..29406)
                     /locus_tag="VFQ79_11690"
     CDS             complement(27499..29406)
                     /locus_tag="VFQ79_11690"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HEU0140372.1"
                     /translation="MSTVPTELPLKASEAAALCQVILEQIDGREPTGESRALIATRIA
                     ELNLDTIRAYPGSLEMDPAHPSTYYLAIDALTETRRVPLLIHVTPASSPAAPLFSNAV
                     SIGRVRPGGEREMLVNAIPFASTDHEQIRRFVLELDRSLLPRPQGAQPAIAVGNRHPE
                     ISLPAAFQAFQKILDETGINLASTVQLSATREMTTEEALANRDGENPVSAGHTRVSIR
                     HLYHAGLWAAIRCGFRHGYNAEADHIIITGDNDSQIAQSVEVGKEAIRHASGYTKFTT
                     DTSRLFELQADTRHPNPWPDSVVRERYEQIFDAGERSWIEQEFAGTFDTGETSYVLRR
                     EEIVRLAVKFGRSLKLNEELYDYIQQVKEQEPARRTFDFEPSLDEADTLTTPQELIFY
                     MHWLKTRGRAAQLVPPNLGFKKRQAYPMAFKTGPESGVGLEDYVQHKMWPELLPRVIG
                     EYGGKPLEELAARVSLLAAVARRFNGTLSVHSGSGKQAEVLEHIGRSTAGRVNYKISG
                     ELQLQLFDVLSEQPAGSRWRQLYERMADRCNRFAGQGVFGAESELAAKYLDMGRGYYL
                     GNAAAGRVDGNLFLVFWVGNMVGTRDTAAPDGDRRFFKEKIDELPEELVQEVQRRNAE
                     YIVWLARHLKG"
     gene            complement(29415..30299)
                     /gene="galU"
                     /locus_tag="VFQ79_11695"
     CDS             complement(29415..30299)
                     /gene="galU"
                     /locus_tag="VFQ79_11695"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU0140373.1"
                     /translation="MLPKVRKAVFPAAGLGTRFLPATKAQPKEMLPLVDKPLIQYGVE
                     EAIHSGIQNIIIITGRGKTAIEDHFDVSFEMEHLLESRNKKDLLNTIRSISDMINVAY
                     VRQKEAMGLGHAVLRARELIGPEPFGVVLSDDVIDAEVPCMRQLLDIYEFYGASVVAL
                     MEVPKDIISSYGVVDAEPIAHNGARDRLFRICNMVEKPRPDEAPSNLAIIGRYVLTPE
                     IFDSIESIEPGSGGELQLTDALKHLLRSRPIYGLRFEGTRYDAGDKLGFLKATVEFAL
                     KRYDLGEEFRQYLSALKL"
     gene            complement(30373..30750)
                     /locus_tag="VFQ79_11700"
     CDS             complement(30373..30750)
                     /locus_tag="VFQ79_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-[acyl-carrier-protein] synthase"
                     /protein_id="HEU0140374.1"
                     /translation="MIAGTGVDLVEVHRIRQSIERFGARFIERVFTPGEIAYVERKAN
                     RFERYAARFAAKEAGMKAIGTGWRHGVRWQDFEVVNERSGRPTLQFHGVAAEFAAALG
                     IRRVSLSLTHTAQQALAYVILED"
     gene            complement(30747..31163)
                     /gene="nikR"
                     /locus_tag="VFQ79_11705"
     CDS             complement(30747..31163)
                     /gene="nikR"
                     /locus_tag="VFQ79_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEU0140375.1"
                     /translation="MSELSRIGVAIDSDLLAKFDELIANRGYTNRSEAFRDLIRDELV
                     EKAWEGPESPVVGTVTLVYDHHVRLLSDKLTDLQHDHYHLILSTMHVHLDHDNCLEVL
                     VVKGRAKDVKKIADALISTKGVKHGRLTITASGAEL"
BASE COUNT         6035 a         9546 c         9401 g         6272 t
ORIGIN      
        1 tcgcgcaaca ggccgcgccg cccgcatccg atgttggcga cccggatgcg cccgttcgcg
       61 ccgggagtgt tctgccaggc ggaagccgtc atggacgctg ccacagattg tccaatgaag
      121 tatcttcggt tcacgagtag atgtttcctt tcgtttctca ggtgacatat actttatccc
      181 aaatggaacg caagcggaga ggggggagcc tttcatgaat cgccgtgagt tcgtttcaag
      241 cagtctgctg gtggccggtg cggcgggttc gcacgcgcag atccgtacgg gattccaggc
      301 gggttacgcc gaacgcgaca tcacgcccgc tatcggcatg gagcagcccg gaggctatgg
      361 caagtcgtat cacacgaaac tccatgaccc gtgtaaagtt cgtaccgcgg ttttcgatgg
      421 aggcgctacg aaggtcgcgc tggtgggcgt ggacgccggt tccgtcggcc ggcgtatggt
      481 attggcagcc cgggagggca tccgcaaccg ttgcggcatt ccggctgacg cggtcctgat
      541 cggcgcatcg cactcgcatt cgtcgggacc gatcggcggt gtcgagccag gggaatatga
      601 ccacgcctcc gccctggtcc gggaactcgc ctacgaaaag tccacggtca acaatcccaa
      661 gtatgtcgag cacgttcaaa atcagatcgt cgacgccgtg tgcgcagccg ccgggagcct
      721 ccggcccatg gccagcgggg taggttcggg aatcgaggac aaggtcgcgt tcaaccgccg
      781 gttccggatg aaaaacggcc tcagctatac gcatccgggg caaggcaatc cggacatcgt
      841 gaagccggcc gggcccatcg accccacggt gggcgtcatc ggcagttggg atgattccgg
      901 caaactggca ggttgcatcg tgaattactc ctgccatgcg actaccaatc cgggaggaat
      961 ctccgccaac tggatctact acatggaacg gaccatccgc ggcttcttcg gtcccgacgt
     1021 cgtggtcgtg ttcctgcaag gcgcttccgg cgatgtcacg caggtggata acctcagccc
     1081 atatcgccgc gccagcggcg cacgggaagc gcagtttgtc ggtggacgag taggcgcgga
     1141 agccgtaaaa gtgctgctta cgatgcaccc cggcgaactc acaccggtca cttcggcatc
     1201 gcgcctgcta cgcatcaaac ggcgcacgcc ttcggcggag aaagtgaagc gcgcttacca
     1261 gattgtcggt caggatccga aagaggccgg catgactgcc tggacgttcg cgaaggaaac
     1321 ggtgatgctc gacgcgaaac tggctaagga gcctgtcgcc gacgtggagg tgcaggctat
     1381 tcaggtgggt ccggccgttt tcctcagcaa tcctgccgag ttgtttgtgg agttcggact
     1441 cgaacagcgg cgccgcagcc ggtttccatt tacttttccc gtggaattgg cgaatggaga
     1501 tgcaggttac gtaccgacgg aagaggcctt gggtcctcat ggcggcggtt atgaaacaag
     1561 gctgacgagt tacagcaacc tcgttcccac ggcgggacgc cagatgacgg aagccggtct
     1621 cgaactcgcg gatcgcatgc agcccggcaa ggttcccgag ccgccgcgcg cagagccctt
     1681 caagtcgcgg gaaggtggaa tcggttccga agcgtggtcg tacgggaacg tgccaccgga
     1741 gttgagttaa ccgttccgct cagaacagcg tgtagataaa cggcgcgagc ggcgagcttt
     1801 gtgccagtac gatgaaaacc gcgaacacca gcatggcaac cacgatgggc gtgatccacc
     1861 accacttgcc gcgccagaaa aacagcagca attccgaagc gatactggag cgggcggcga
     1921 ctctccggcc gaaactcatc ttcgttcgat cttagccgct ctggtcccgc ttctcaacca
     1981 ggaacggacc cagcaccagc gtgtccatgc cgctgcgctt ccaggatgcg caggcttcct
     2041 ccggcgtgtt cacgatgggc tcgcccttca agttaaaaga agtgttcagc aggactggtg
     2101 tgccggtggc ttcgccgaag gcgccgatga gccggctgta cagcgagccg ctctccgaag
     2161 gaacgacctg cacccgcgcg gtgccatcca cgtgcgtcac cgctgggatc gcatcccgct
     2221 tctctgcgcg caccgggact acgtacagca taaaccgggc cgggtattga cgctcggggc
     2281 cgggaagatc gaaatattcc ggggcgcgag gaagagacac ggcgggagcg aacggccgga
     2341 actcctcacg aaatttgatt ttctgattca ctgtgtcttt catttgcgcg ccgctggggt
     2401 cggccaggat gctgcggttg ccgagcgctc gaggtcccca ttcgaagcgt ccctggcacc
     2461 atccgataac gaggccgcgc gcgagctggc cggtggcttc ctggatgagc agaccctcat
     2521 cgcggaagca gcgataatgc agtccgctcc cgtcgagaaa cttgcggatg gtgtcgggcg
     2581 aatgttcctc tccccaggcc gcatgttcca tcacaaacgc gcgcggctgc cccaacacca
     2641 tgtgatgcgc atacagtgca gcgcccagcg cgcctccact gtcgcccgcc gcgggttgga
     2701 tgtaaatttc atcgaacaat ccttcgcgca gcaggcgccc gttagcggcg ctgttcaacg
     2761 cgacacctcc agcgaggcac aggcgcctca tgccggtcag tcgctgtgca tgcgccgcca
     2821 tccggaccac gatgtcttcc gtgacagcct ggatgctcgc cgcaacgtcc gcgtaccggc
     2881 ggctctcttt ttcgcgtgga tcgaagcgct gcgccggatt cctggggttg ccgaacagat
     2941 gctcgaagcg tgtattgaat gtcgcttcca acgaatgctg gaacgagaag taatcgagat
     3001 tcagctcgaa gctgccgtcg gcattcaccc ggatgagccg atagacttcg tccagatact
     3061 tcggctcgcc gtaaggcgcc atgcccatta ccttgtattc gccctcattg acttcgaaac
     3121 cgagaaaggc cgtgaaggca ctgtacaaca ggccgagaga gtgcgggaag cgaatttcct
     3181 cgagcaggcg gatgtctgta cttcgcccca ctccgatggc cgcggtggcc cactcgccaa
     3241 cgccgtcaat ggtcagaatc gccgcctctt catagggaga gcagaagaag gcactggcag
     3301 catgcgacag atgatggcgg ctgaacagaa cgcgcgaatg cggcagcccg agacgccgcg
     3361 ccagcagatc tttgatccac aacttatcgc ccagccagga gatcatcgcc tcctgaaaca
     3421 ggcgccgcga gcgcgggaag ccctgtaaac aggagacgag aagccgttcg aatttcagaa
     3481 acggtttctc gaagaagacg acgtagtcga gatctttccc ttcaaggcct gcggtgttca
     3541 aacagaattc gattgcccgc tggggaaaag aaggatcgtg tttgatgcgg gagaatcgct
     3601 cctcctcggc ggcagccaca agctggccgt ccaaaagaag cgccgcggcg gcatcgtgga
     3661 aatagcagga gaccccgaga attctcacgc gctaggactg ctcctgtgcc cgttgctgtt
     3721 cgctgatcga aggctccgcc ggcgctgtcc atcctgcgct gtgcgcctct cgtacgcgga
     3781 cgaggagcgc aaagggcgcc agcagcgtgt aatagaacaa cgcgagcagc atccggattt
     3841 gaaaatcggc aatgcggcga gcgacccttt tccaggagtt ccacattcgg cgcaacatgg
     3901 caatttaccg gcgacgcgcc gattgtagcg ctttgctcag gttctggcgg gcgggcgcat
     3961 agtccgggcg cagacggatg gcctcggaga aatggtggac agcccgcttc atgtcgcctt
     4021 cacgtgcgaa ggcggtgccc atattattgt gcgcttcggg ataatccggc ttcaggcgga
     4081 tggcgtcggc gtagtgcacc aatgcttctt tgagccggcc cgcgcggctg gccaggagcc
     4141 ccaggttgta gtgcgcctcg gcaaattggg gcctttggct gaccgcgatg ccatactcac
     4201 gagcggcctc ttccgtcttg ccctgctgca tcagtacgcc accgaggtta tagtgcgcgt
     4261 ccgcgaactc aggctgatat ttcacggcgc tggcgaacga cgcctgcgct cggtccagat
     4321 ccccccgcgc aaggtggatc aagccgagat tgttgtgtac ctcggcataa ccgggattga
     4381 tgcgcagggc ttctccgtac cggagcaccg cctcctgata attgcctttg gcggcgaagg
     4441 cagagcctaa tccgaacaaa gcgacgtcgg aatcgggatc gatccggagc gcctcccgat
     4501 attgcgagat cgcctcatcg atccggcctg catctgccag cgccgccgca tgcgcggcat
     4561 accagttggc aatgccgggc aggcggctgt tcaaaatgcg ccactcctcc gccgctctcg
     4621 cgtactgccg ctcatcacgc aaaaattccg cccaccggac gcggagcgcg aggtcggccg
     4681 ggcgctctgc gagcgcgttt tcgtaaatgt gccgggcgtg gtccatagct cccgcggcac
     4741 ctgcattctc ttccactcga tgcagtgctt cttcgcgctt gctgcgtgcc tgcgccgaac
     4801 caacctgatt cgtgaacggt ggacggtcca tcatggcatg catctggcgc gcgagtttgt
     4861 accggtccca atccgtaagg gccagttcgg cggccacagc ttccatgaaa ggcatgctgc
     4921 cggtgcgctc gatccatcgc ggtgcgatgg aatcaaaccg ctgcgcgatc agccgggcga
     4981 gttcgtaatt gccttgttcg ttcaaatgca cgtgttcgta aaaccgctca tggcccgcca
     5041 ggccgaaccc ggcttccgca tccacgatat agacgcgggc gccacgcagt ttccggatga
     5101 tgccgttcag gacgctatcg gttcggaagc gtagcaggtc gcggtcgcga gcggctatga
     5161 atgcatcgcg tgccgcgccg gtttgattca gcgccaggta gcagcgcccg atgcggaaat
     5221 gcagttccgc ggactcttcg tccagagctg ctgcctgctt ataagccgcg agagctttgc
     5281 tccattgttc cgccatttcc agttccaccc cgcggcgata gagtccttgc cagcgctcgt
     5341 tgtcaggctg cgcggaggcg aagggcgggc tgtctttaag attgctcggg acggtagaga
     5401 agagaatggc cgccccggct cgctcggccg tttgcgcgat gtcctccagg ttgcggcgga
     5461 aattatcgta ggccgcatga agaggaccgg cgtcccgcgc tacccgcttg tcgagaaaca
     5521 tctccatacc gcgccattca acggtctcgg gttgccttcc aaacctcgcc aggacgttcc
     5581 ggaacagttg tcccgtgcgc gtggaggtct gccacacatt cacgcgggcg aaccacagcg
     5641 ccggcggcat agtgccgaac acggtgcctg gcccgaaggg acctaccact tcgttattgc
     5701 cgatgagcgc tacgaaaaga tcgggctgga aggatgcgca gtcgcgtgca ataggcagaa
     5761 tcgcgtggga gttgatcgcc gtcatggccg cgttcaccac ttcgaatttg gcggcgggat
     5821 acatctcgcg caacatcact tccaacaccc tgccaaagct gtacgccggt tccggaacgc
     5881 ccattgcggc ggattcgcca agcacgaaga tgcggtaggt gccaggggac ttctgcttgt
     5941 tcaacagcgc gggaaccggg gtgcgggcca gagccttggg aaaaaaccgt tcgccgaatc
     6001 tttggttggt ggtgtattca ccggattgcg cggccggcac gaaaaactgc gtcgcgaagc
     6061 cgtaatccga aatccggaga acggcctcaa acaacgcaaa agccgcaacg ggcgccagaa
     6121 agatcagcgc gattcgcggg atccaaccgc gcatggacca cctctagttt cgcggagcgg
     6181 cgactttctg gaataagtcg acaatgccct cgacaatttt cggcgcagcc ctggtctcga
     6241 gcaggttggt gccgcgccac gtctcgtagc ctcccacctc gtgttgctcg gcggtcggca
     6301 ggtagccgaa ccagccgttt gccaactcta tggtgaacgt ctgtttcagc ggactgcgct
     6361 tccggatttc gagcccgatc tctacaaaga cctcggccgg gaccgccgcg atgccggcat
     6421 cgccgatccg aaacgtctgt agtggaacgg gagcttcgcg cggccagtct ttcattctca
     6481 acaaacgctc cgcatacacc tgttcatgct gatggtactt cggcggatct ttcagagtcc
     6541 tctgtgccca ttccacctgt tcggcatccg gcacgcgcac gccgagcatc aggtccttct
     6601 gcgcagcggc gagcggaacc cagtcccggt gttccacctt ccggtaggcc tcaaacacgc
     6661 ccgtggcgat ttcgtctgca accatacgca ttttttcata gggctcgaac cggcgcctgg
     6721 cagcatccgg accggggcgg aagttgatgt tgttgatgtt gccgctggtg ccgttggaga
     6781 ggattcccac aaatggcggg ttctggttcc gcgcgtttag cagctccgca atacgttcgg
     6841 cgaacacggc aaaatagtca cccgaaatgt cgccgccttt cacgccgcca acgtaatgca
     6901 gcgagtagtt ggccagcagc gcgatgggct tgccggcagt ggactgtacg gaaatgaagt
     6961 tgatttgggg atcggtgggg ccggccggct caatcaagtc gggatcgttc gcgggcggat
     7021 tcatgcgaac cttatcgatg ccgccgaaag gattccgcag gtgcttgctt cccggtttca
     7081 ttttccagcg gcgattgaac acctgccggt cattgaacgc tactccccag ccgatgcgcg
     7141 ccggttccag gcggtagatc gccatcagga cggcatcgcg aatgcgatac gcgaggaacg
     7201 tctgatattc gtcgagcggc gcgggcgtga taccgcggcc gccgcgggcg ctgctggcgg
     7261 agtgggtatg agtggcagcc atgagtatat tctccggcgg caatcccact ttttcctgta
     7321 ccatgcgctt ggcttcgtcg aatacgacac gcgtgatgcc gacgctgtcg catagcacga
     7381 tggcaaggcg tgtggatccg tcgtcaagca ccaacgcacg cgaatgcagc tcatcgtgaa
     7441 cgttggtagc gggaggcgaa ttccacccgc cgacaatggg cgagcccaac ggcggggtaa
     7501 tgttcgtggt cgccgcaccg gcgcgaaaca cacgctcacc cgccggttgt gccctcaagc
     7561 cggcagcggt catcagaacc gccgctccga acagaacgag acgtttcatg ggagtccccc
     7621 cttccaatcc tgcaactgac gtttcaatag agataacaca ggttcggcca aatccgcatg
     7681 gtgcggctat ggtacagtac cgccatgcgg cggctggggg tgtggttctg cgcgttgttt
     7741 tgcaccacag gcgttttttc cggacagccc gcctatacgg acgccgcgga atgcgccgca
     7801 tgccacccgg cgattgcgaa gacctacgag cgaactggca tggggcgttc tttttatcgc
     7861 gcggcagaag attacgtgcc ggatgggtcc tactatcacg aggcctccga tcgatactac
     7921 gcggtcgtgc ggcgcggcgg cgaaccgttt ctccggcgat ggcagcggga agcaaacggg
     7981 ccggaattca acatcgaaga aaagcggatc gactacgtgt tagggtccgg caatcacgcc
     8041 aggagctatg tttcgcgcgc gccggatggc cgccttctcg cgctaccact cgcctggtat
     8101 gcggaggacg ggggcaaatg ggcaatggct cccggctacg accggcccga ccaggcggat
     8161 ttccgccggg agatcggctt cgactgcatg ttctgccata acggatatcc ggaaatggaa
     8221 agcggcgcgg ccagattcgg gaaaccggca cggttccggg ggcgcatacc ggaaggcatc
     8281 gattgccagc gctgccacgg gcccggcagt gcgcacgtgg aagcggcacg gagcggagcg
     8341 gggcaggatc gcgtgcgggc ggagatcgtg aacccggccc gcctcagcca cgcacggcag
     8401 ctcgaagtat gcctgcaatg ccacctggaa tcgacttcgc ggcgtctgcc gtatgccgtg
     8461 cggcgctatc atcgggacat cttttcctac cggcccggcg aaccgctcgg cgactatatg
     8521 gtgcatctcg atctggcgcc agggtcgggc cgcgcggaga ccttcgaggt ggtaaatgcc
     8581 ggttatcagt tcctcaagtc cgcctgctat cgcaagagtg gggaacaatt gacctgtacc
     8641 acctgtcaca atccgcacga tgtggaccgc ggcgacgcag cccgcacacg cattgcatcc
     8701 atctgccagg ggtgtcacga aaacgtgcgg catcgaacgc cggtcacata cgactgcacc
     8761 ggatgccaca tgccgaaaca gcgaacggac gatgccgtac atgtcgtcat gaccgaacac
     8821 ctgatccgcc ggacaccgct tccggaggat ctattggcgc cgaaacgtga agtgcatgaa
     8881 accggcgagc atacgcacct gggaaaagtc gtcccgcttt atccgaagaa gctgtccacg
     8941 cttgatgaac tgtacctggc tgttgcacag gttcgtgaaa gcgcaaatct gcgggaagga
     9001 atcccgcgcc ttcaaaacgc gctggagaag ttcaggccga aagaggcgcc gttctatttc
     9061 gagttggggg aggcgttgcg gaacgtaggc cggcatcgtg acgcggtgcg ctactacgag
     9121 caggcgatag agcgcgatcc ggatttcctt tccgccgcac ggggactagg ctcggccctc
     9181 cacgctgccg gcgactataa ccgcgctatc gccgttctcg agaaagcggc ggaatcagac
     9241 agcgaaagtg tcgagacctg gaacgtactt ggctccgctt atgacgcggg ggggaggccg
     9301 gagaaagcaa tggcggcatg gcgccgcgcg atggaagtga atcccgacac accggaaccc
     9361 cacttaaatc tcggtgtggc cctttcgcgc tcgggcaacc ggcaggcatc ggccgcggcg
     9421 tttctcgaag ccatccggct gcgtccggat tttgccgcgg cgcacaataa cctggctgtc
     9481 gtgctcgagc agcaaggcga agatatacgg gccgcgcggc atttcgagcg ggcgatccat
     9541 ctcgatgccg cctatgccgg tgcgcgctac aactacggag tgtatctggc ccggcgcaag
     9601 cgctgggcgg aggcgcggtc gcaactcgaa gaagccctgc gcctacagcc cgcggccgag
     9661 gtgcgcacgg cgctcgaaat ggtgaagaag ctggaagacc aattcacgcg ctgaatcttt
     9721 ggttttaacg agccagtgca cgtttatcgg caaaggatcg tctgagcgta tggagacttc
     9781 ttatgggaac ggcactgcgc attttgctca tgttgttgac tgtcgccggc ttcagcctgg
     9841 catcggagtc catcaggatc tgttatccgg acggagtatt ccgctttggt tcccctgtgt
     9901 ccaccgcaga caaggggttc attctgtcgc gcgcgttcga atcggtggat ggctcggcgc
     9961 cgaacgtctg ggtttatgac cgggagggca aggccgtcac gagcgcaatc ctgtggctcg
    10021 aggacgctgc caaagtccgg gtgttcgacg tgggcgtcga ttacgatgga accgccgtag
    10081 cttcggggta tgtgacgcgt gcggacggca cggccgaggc cgtcattggc attattggcc
    10141 ggggcggccg gatagaacgc gtgatccgga cgaacccttt cgtggcgcga tttgtcaccg
    10201 tggggccgga tcgggtcatc tgggcggccg gacgggacat cgaagcgacc cagcgagggc
    10261 gggactatga catggtccgc agctacagta tggatggccg attcttgcac tcggctgtgc
    10321 cgcgatcgag cgccgcgtct gtccaggatc ctgtcccggc aactggcaaa acaggaagaa
    10381 cacacttgcg cggctcctcc accaacgtag tcttttacag cggagtgatg aatcggctga
    10441 tcgtatttga tcgagccggg gcaatcgtga acgactggtc tcctcccgtt ccctggcgga
    10501 atgccagaat gggagggttc ggaaccgatg ccgcgtcgat ttacgccctt ttccggggcg
    10561 acgacgggca ccggggtgcg ttccggttcg atcgtcaggc agggaactgg gtgcttatca
    10621 aagacactta taccccgccc ggtgaacgcg tccgtttcgg cctgctgcgt ggaacggacg
    10681 cgggcgccct cgttttcaca aaccgtaaga tgccggagag ttgcgtgacg cggttgccgg
    10741 tcccgggtcc ttgatccgaa tttacaccgt tttccagccg agcaagcgag cggcgttgtt
    10801 gtgatacacc ttctttaaaa tcgcatctgg caagccgatg ccgtaaatgc gccatcggcc
    10861 ttgcgggggc gtggcgctcg gagcatagtc gaagtactcg tccaatgttt cgagaaagcg
    10921 gaaataggcc tgatacatcg cgggcacaag atcctgctgc gggacctctt tgccattggg
    10981 cgtaccatcg gttccgaaca tgatccggtc ttgaaactgt tcgaagaact tacgcgcgcg
    11041 gcgcggttgc cggccgattt cacccaaccg ggcgccgacc tcgcaatgca gattcgggta
    11101 ctgcttcaac caggcgctta cttcatcgag gttctcgggg cgattcgcca cgtgcaggcc
    11161 gataaactgc gttttcgggt gccgcgcgac aatgcggttc cgtgcggcca gcagctccgg
    11221 tttgggtgga aaatcctttc cgtaaaacga ccagtcggga tgattgccca actcttccca
    11281 tcgttcattg aaacgatcgg tgggtgtaaa gaacgcatcc gggtccgcga cgtggataaa
    11341 tacgggcatc ttcagcgccc ccgcctgttc ccacatggga tcaaagcgcg gatcgtcgat
    11401 ctttacgagc ggcccttccc ggccgttttc ccgtaagtac aggcccagcg tcttcgaaat
    11461 cttcaacccc accgcgccgc tcttcttggc attggcgagc tcgtcggcct gccacttggg
    11521 atagtcctgc tcgcgtatcc tgtcccaggc cggcgttgtg caggtgacga aatggctgtt
    11581 gtagcgctcc tggagatcgt tgacgttccg cttcagatcg tcgccccaac ccccggtcac
    11641 gttgatgatg cgcaccaggt tgatctcttt ggtccacttc gcgatttcgt caagttgctt
    11701 cagagctgtt tcacggtcga tgggcggagc cgagcggcgc gagcggaaaa tactcgagat
    11761 gtgcgtgtgc acatcaatca cgggaaaacg cgcccggttg acggggtgct caggcaccac
    11821 cagcatgctc ttcggttgga attcttcgag gacaagttta tccggcacgg ctttttgcgc
    11881 ctgaaccgat ctctccggcc tggagcacga gccggccgcg gctacgattc ctgcggcacc
    11941 aaaaaaggcc cttcgcgacg ttcgtttcga catgatggta ggactccttt tgagaaaact
    12001 aaatcacgat tgtaatccag ataaataggc ccggttgtaa atagggctgg attcatgatt
    12061 ttcaaccccc ctcgatgtta cacttccacg aagatctact ctgaattcga atcgaaggag
    12121 ctggacatgc ctgttcacga actggggatc tacgttttca ttctcgccgc cttcctgggg
    12181 ctggaactga ttcgccgtgt ttcccctctt ctgcatacgc cgttgatgtc cctgacgaac
    12241 gccatctcgg cgatctccgt agtgggagcc attgtgatcg ctggcgagga gaacgcttcc
    12301 actttcagca gggtgctggg attcatcgcg gtaaccaccg cgaccatcaa cgtggtaagc
    12361 ggatacctga tcacagaccg gatgctgaag atgttcaaaa agcaggagag aggcaagccg
    12421 taatgtccct tatcgtttcc ttcagctata ttgccgcggc agtactcttc attctggcgc
    12481 tgaagtggat gagcgctcca gcctctgcgc gcaggggcgt ccgggcgggc gaactcggga
    12541 tgctccttgc cgtcgtcggg acgctgttac gcaacgaggt ggtggattat gagtggattg
    12601 ccgctggtct cctcctgggt tcggcaatcg gcgttccgct ggcctacctg atgccgatga
    12661 ccgctgtgcc gcagcgcacc gcgatctccc acgcttttgg agcgctggcc gccgcgctga
    12721 tcgggaccgc ggagttctac aagcatccac aacatggctt cattctagga gcgctggtga
    12781 tcgaggtgct tctcggctat ctcacgttta ccggcagtct gatggcgttc ggcaagcttc
    12841 aggagatcct gccgggccgc cccctggtct acaagggaca gaacatcgtg aacctcacgg
    12901 tgttcgccgc cgcggtattg gccggaatat atctgattgc aaatccgtcg gctacctggg
    12961 cgttccctgt tctttctgtg cttgcgttgc tgtttggcat catgctgatc gtccccattg
    13021 gcggcgccga catgcccacg gtgatcgcac tgctcaattc ctatgccggt ctggccgcca
    13081 gcttcatggg gtttgcgctc gacaactacc tgctggtgat tgcgggcgcc ctggacggcg
    13141 cctcaggctt gattctctcc ataataatgt gccgggccat gaaccggtcg ttcgccaacg
    13201 tgctgttcgg ggcgttcggg caggttcaag cgaaggccgg cgcggtcgaa aagcgcccgg
    13261 tgcgttccgc cgagcccgag gaagcggcgg aaattctgga ggcggcaaga ctggtgatta
    13321 tcgttcccgg ctacggcatg gccgtggcgc aggcgcagca taaaatccgg gagttgtacg
    13381 accgcttgac gaaattgggc gtcgaggtga aattcgctat ccatccggtg gcgggacgca
    13441 tgccggggca tatgaacgtg ttgctggccg aggcggacat cccctatgac cggctgtccg
    13501 agttggacga gatcaatccc gaatttgccc aggcggatgt cgtactggtg gtgggcgcga
    13561 acgatgtcgt gaaccccgcc gctcgcacgg acagcagcag tcccatttat ggaatgccga
    13621 tcctcgatgc cgataaggcc cgtaccgtaa tggtcatcaa gcgaagcatg aatccaggat
    13681 ttgccggcat cgagaacgaa ctctattaca tgaacaagac cctgatgcta tttggagatg
    13741 ccaaagcggt gttgggcgaa atgaacaaga gcctcgccgc gcaacgtgtg tagcctcccg
    13801 atgtgaccgg gatcactgcg ttccggaagc agcccgctta cagtggggta tcatgcacag
    13861 aacgccgatt catttcctgg cgctggccgt cctcgcggct ccacttgcat ttgctcattg
    13921 tgacggtctg gacggcccgg tggtggcagc cggacgtgaa gctttgcggt ccgccgatgt
    13981 caacctagta atgccctggg ttcagcccgg cgacgagcct gcaatccggc aggcctttgc
    14041 ccgcgcgtta aaggtgcgca agctgaatcc cgatgcagcc gaattagcag acacgtggtt
    14101 tttcgaaaca ctcgtccgga ttcaccgcgc gggggaaggc gcaccttacg acggcctcaa
    14161 gcccgccggc cgcgaccttg gccctgccat tcctgcttca gacaaagcca tcgaaaccgg
    14221 tgacgccgat gcgcttgaaa agctgctctc ggcgccggcg cgggaacaac tgcacaagcg
    14281 gtttcgccgc gttgtcgatg cgcggaatta cagctccgtc gatgtgaagg cgggacgcga
    14341 gtatgtggcg gcttatgtcg atttcgtcca ctacgtcgag cgtctgtacg aagccatcac
    14401 cgccggcagc gggcatgcgg ccacgcacga gcattagaac ttgccttatc tactgaacgc
    14461 gcacttcgac ggtttgcgaa acggccttgt ccgaatcgtc cacggccgag agcgtgtaca
    14521 cggttgtggc gtcgggcttc acctcaacac aacggctgag cgccggatga acctcctcca
    14581 cgcccggctc gatgtgaacg gattttgcgt tcaggacccc gtagcatagc agtcctgtat
    14641 cgccgcgctt cagcacaccc gggctggcat aaaaggtcag gattttcagt tcgctcgccc
    14701 catactgctc gacgattttg cgatcggcct ccgtacggct gcgctcaatg gcctcaacgg
    14761 cggcgcgatt gtgccgccag cgcaacacaa acaccgaggc cgtgtacaaa agcgccagca
    14821 ccaccagtat ccaggtccat cgaagtagcg cacggccgcc accctgtgcc attcacccat
    14881 tgtattcaga tcgcgcggcg ctacactgcg gtgaggcgtt ttccacggca gatggtcagg
    14941 cggctgacaa ctttttcgtt tacctgaacc acgtttctct gcgaatcaac caggcggaaa
    15001 tcacccttct gcatctccag cagcgcaaca tcggccgggc ctcccacggt caacgatccc
    15061 attcccggaa ggcgcccgac gactttcgcc ggattcgctg tcgtgcgcag cagcacatcg
    15121 tcgaagctca gacccaggtg gaggtacttg gtcatggtgg tcggcaggtc gtaagtgggg
    15181 ccgttgacgc aggccgaatg caggtcggtc gagatgcagt ccggcaggaa gcccgcgtgg
    15241 attgccgtcc gggccgcgtt gaagttgaaa ctgcccgcgc cgtggcccac gtcgaacagg
    15301 acgccccgct ccctcgcttc cgccacgccg ggtttcagtt gccccaactt tgccgagtaa
    15361 ccgctgtcca caataccgag cgtatggccg ttattgcaat gtgtgaccac atcgccggcg
    15421 cggagcagtt cgagcacctg gtgaatctcc ggaggcgcta aactgatgtg cgccatcagc
    15481 ggcagcttga actcgtcgcc cagctcgcgg gcgatcttca gaaaatcgta gctatagcgg
    15541 ccgttcatat tcgagccgac gaataccttg atgccgagca cgatgtcgcg attggcgagt
    15601 gcggcctcac ccgtgcgccg tacgccgctc cgcacatact tgacaacatc cacatccgga
    15661 ttggtcaggt gcgggtacag atgcatatag ccgaagattc ttgtccgcga agcatccacc
    15721 gcaaagcggc ggaagccgat gacctggtca taggcgaaac tgccggcatc cacccacgtt
    15781 gtggtgccgg agcgggcgga gatggggtcc gcttcaatgc tgacgccgcc gcctccgtag
    15841 aagcagtgcg tatggaggtc caccaggccg ggggtaacgt acagcccttt ggcatcgatc
    15901 acatccagag cccgatcggc ggagatgttc tgttcgatgg cggcgatttt tccatccagg
    15961 atcgctacgt cggcctgcag gcggtagttg cggccggggt cgcgaacttc gccgtttcgg
    16021 atgaggatgt cgtatcgccg ctgaggacgc tggccggact ggccggacaa agattgcaca
    16081 acggccgcgg cggcgcccga tgccagaaaa gcacgtcgaa tcatggcccg gattatatca
    16141 gagggatgga ccggagtttc agttccaaaa tacttgaata tttggtcttt ttaggttaat
    16201 attgaatcgg ttagcgccca ctgaagtgat tcgcgcgccg gcgggagtgc atgccaatgc
    16261 ccgtagcaca agaagtctgg atatgcaggc actgcgaaca ggttgtggac cggaacgcag
    16321 ccgtctgcca ctcctgcgga acgatgttcg taccaggaga agacattatt ccgctgcgtg
    16381 aactcccgcc cgatggagcg atgcgctgtg cgtattgtgc gcaagtgctc gaccccggcg
    16441 cggcagtgtg ccattcctgc ggatgcgtac ttccgcgcag gggatgagcg cagaacatga
    16501 aggagtttcg aaaatccaag ccgatccggg ttctggcaaa aacgttttcc attattgagg
    16561 ccgtacagga ctcacccgca cctctgacgc tgaaagccat cagtgagcaa accgccgtca
    16621 acaagagcac ggcctcccgc attctggccc acctcgaaac ccagcgatac gtcacgcgcg
    16681 atgcgaaggg cggctatcgc gtgggggaga aactgcttca actcggtacg cgtccggctt
    16741 cggaggtgaa ccttcgagaa gccgcgcagg cgcctcttcg cgaactgtgg cgcatcacgc
    16801 aggaaacggt aaacctgggc attctcgatg gcctggaggt gatgtacctg gatggtctgg
    16861 agagccccca aagcttccgg ctggtgtcga gcgcgggaac ccgcgcgatc gcttaccgca
    16921 ccgcattagg caaagcgata ctggcatacc tgccggccga agagagggaa gccgtcatca
    16981 cctcgttcgc tttccagccc tatacgccgc acaccatcgc ttcggcggaa cagcttcgta
    17041 atgaactgga acacgtgcgc gcaaagggat atgccatcga cgatgaggaa agcgtgatcg
    17101 gtgtccgctg cctggcggcg ccggtcctga gccctgcgcg gaccgcgacg gcggcagtca
    17161 gcatctccgg gccggtagcc cggatggcgg acgacaaaat cgaagagttc tccagggccg
    17221 cgcgcgctgc cgcccgcgag atcacggaac gcctccgcac aataggccaa agcagactac
    17281 cagaccgccg attcgtccaa cagcggcagt gtaaccacag cgcctgattt cgccgaaaca
    17341 agcgggttgc ctcttgccct attcagtaat ttgatatatc gtaattacga atatggctcg
    17401 gaagccgcag tccggtccag catctgatcc tgaaatcttg attctgtcga gccttgcagg
    17461 cgggcctaag cacggctacg cgattctcgc cgatatcgac agcttcattg gcggcaggat
    17521 gggacctgga acgctctata cagcgatcac ccggctcgtg gataaaggat ggatcgctcc
    17581 tgaatctccg gccggtcgtc aacggcccta tcgcctgacc gccgagggtg cagcctgtct
    17641 ggaagagcta ctgaaaaata tgcggcgagt ggcaaaaata ggcctggaaa ggttgcgcct
    17701 ggcatgaaca gtttgaaagc gcagattgtg gcagcattgg ccggcctgta tccccggcga
    17761 tggaagcggg agtatggcag tgagttcgct gatgtgctga tcagaagtcc cttgggcccc
    17821 tccgcagtgc tgaacattgc ttggaacgcg ctccgtcagc agttgcgctt gggagagccc
    17881 tggctcatcg taggaattcc atggttgtta ttgaacctct acgggcacct ctggaatatc
    17941 ctctacccag ttccttatgc tgccgatcca tccagtaact cactggccgc gcgggccgta
    18001 gctgggctac ttccgctcgc tatcggctac tggaccgttt tgcgcgacca aattaacgga
    18061 catggcggaa gagcggcgat gaagtacttc ctgctcatcc aatggcctat ttgcgccctt
    18121 gccatacttt ggggccttgg aatcttgaga atcatcgtac tgggccccgg tgatccccct
    18181 tccacgttcc acgaacacgg attcgcctac actttctacg actacccgcg ccgtccaatt
    18241 cattggcaac tgcttttctg gaagcccttt ggggacctcc catttatggg ggtgctcgga
    18301 tggctcggcg gtttggctgc ccgcgcacag gctcgttttc cagggcgacg acggtcaccg
    18361 gggaacgttt aaaagcagac taccagaccg ccgattcgtc caacaacggc agtgtgacgg
    18421 cagcgcctga tttcgccgaa agatacatcg cgaggctcca ttcaagagtg ggccgcacgc
    18481 tcgtgagtcc gatcatcggt tctcggccat taagaatggc gcacgccata tcatcgatca
    18541 caacctggtg atcctggaag ccgagatcgg cggcgccggt tgcgccagtc gccaccgcgg
    18601 cgcttccata cgtgcggatc tcctcgtctt ccggacgctc ctcgcggaac ttccaggtgt
    18661 cgacgcgctc gcccaccatg atggcggttc cgttttcgcc gttgatctcg atgcggacgt
    18721 cggttcccgg ccacagggcc gtggatgcct gtaccacgcc ctgcgcgccg ttctcatact
    18781 tgacgaatgc caggagcgtg tcttccacgt cgatatcggg atgggccagg ttggtcatgc
    18841 gcgcctcgac cgttttcgcc ggaccaacca ggtattgcag caggtccacg tagtgaaagg
    18901 cctgctggat ggtgactccc gcgcccgaac gccgcgacat gcgccaggcg tccgaacggt
    18961 agtattccgt ggagcggaac catttcatgt acgcgtcggc gtgataaatt ttcccgaacc
    19021 gtccagcggc cagcgcgtcg cgaatgacct gcaccgcttt ccgggtccgg cactgcaccg
    19081 taacgcaagc cttcacgccc gccttgtcgc acgccgccgc catctcgtcc acttcgcgaa
    19141 gcgacatcgc cggcggcttt tcaatcagca catgtttgcc cgcggccgcg gccttgagca
    19201 cggcatcggc gtgaagatga ttcggcgtga tgacgctgac gacgtcgagg gtcgggtctt
    19261 cgagcatcgc ttcgagcgag gaatatcctt tgcagccgaa cgtgtccgtg attcgcgcca
    19321 gccgcgcggc atccacgtcc gccgcagcaa ccaactccgc gactttcgaa gcctgaagag
    19381 cgcgggcgtg aaaaggagca atcagccccg cgccgagcaa tccgaatcct acgcgtgaat
    19441 ctcgcacatg gtctccttcg ttattccggc gtcagcagga ctttgagcgc cgtctgcgcc
    19501 tcgatctccc ggatggcgtc gtcccacttt gaaagcggca gccggtggga aatcactttt
    19561 tcgacaggca gcattccagc ggccgccagg ttgatcgtcc ggtcccaact cgtgtagctg
    19621 gtggacaggt tgaagatgag atcgcagacc ttgaacgccg ctttgtccca cgggaacggg
    19681 atggtttgtt tgccggtgag cccgatcgcg cagatgcggc ctttcttgcg caccaggtcg
    19741 atcgccgagg cgatgcccgc cgccgatccg ctgcattcga cgaccagatc cgcgccgatg
    19801 ccgccggtca tctcgaggac cgcctccatc gggtcgatgg tgccgcccgc caccagtgta
    19861 tcgaagccca gttcgcgcgc cttggcgagc cgcaccgctt cgtcctgctt cgcaccgatc
    19921 acgatggtgc ggcgcgaacc gcccgcgcga gccgtcatcg cggcgagcag gccgatgggt
    19981 cccggaccca cgacaaccac aaagtcgccc gcctcgattt tcgcccgctc aataatgtcg
    20041 tgtacggtgt tggccgccgg ttccactacc gccgcggctt catagctgac gttatcggga
    20101 atccgatgca gcagccgttc cggcatcttc agataccgcg tgaacgcgcc gtggattccc
    20161 cagccgggag agcgcttcat gccgcagatc tgtatattcc ccgtgcggca caaatagcac
    20221 tggccgcagg cttgtgtgtg cggctctccc acgacgcggt cacccttttg gaacagggtt
    20281 gtgccgggcc cggtctcgat gacctgtccc gcaaattcgt gcccgagaat cacgggcggc
    20341 cagtagggaa actcatcgtg cagaacgtgc acatccgtgc cgcagatgcc gcacgctttt
    20401 acctcgatca ggacttcacc cgcgccgatc tccggctcgg gcatgtccat taattcgacg
    20461 aagccgggcc ctttctgtgt ttttacgatc gccttcacga ttcgctcctt ccgcaccagt
    20521 tctgagcgaa attgagcagc gccaccgcgg cattgcagat atcgtccatg cggatgtgct
    20581 cctcgcgcga gtgggccacc gagagactgc ccgggccgaa gacgaccgag ggaatcttca
    20641 actggttgtt gtaaaaccac gaatcgcagg aagcggtcat ggcgccgctg ccgccttcga
    20701 gacccgcttc acgacaggca gcggaaagcc ccgcggccag cggatgagag ggatcgagca
    20761 cgtgtgcatc gtgccggtac atgaactcca gcttgaagtg ttcgcggagc cattcatcgg
    20821 cttccgacgc gatcgcctgc ctcatctctt ccatcacttc gtagcgggtc ttgttcggca
    20881 gcaggccgag cactccttcg atgaccgctt cggcgggcgc cgaagcaggc cagtttccgg
    20941 cgttcagctt cccgaaagtg atgggcatgg gattggggta gtcgtcgaac atggcgatgc
    21001 cgcgcgaaga cgcgagcagg cgtgcgtggt agccttccag aatctccatg acccgcaccg
    21061 ccattttgag cgcgctgacg gtgctgcccg cgctgccgct gtgccccggt tttcccgtgc
    21121 aaatcacgcg gaaccagacc gcgcctcgca cggaggacag gatgcgctgg tccgtcggct
    21181 ccatcacgat acaggcatcg gcctgttcgc ctttgcggat catggcgaga gtgccgttgc
    21241 cgcccacctc ctcctccacc acaaggtggg caagaagatc tccgttcaac cggacgccca
    21301 ggcggtgaag cgcggcgatg gcggtaaaga tcgccgccgc ctgtcccttt gcgtcacagg
    21361 cgccgcgtcc gcgcacgacc ccgttttcaa ccaccggatc gaacgggcga ttctgccctt
    21421 ccgaggcggg cacaacgtcc aggtgcgtgt tgaacaacag ggatcggccg cccccggagc
    21481 cgggcacacg tacgcgcaag ttccagcggc cgtcgtattc gatgccgggt accggatccg
    21541 cgtagtcttc gtcctgccgc aggctgttgg agaggggaac gcgctcgact tccgctttcc
    21601 ctgcaaacca cttttcggcg caggcgatcg catctgcttc cttgcccggc agcgacggca
    21661 ctcgaatcaa ctcgcacagg aattgctgtg cgtcgtcacg aacgcttgcc accgcttccc
    21721 gcaattcgct taaagaaatg tctgtggtca tgttgtttcc cgattagagt tgtgccagcg
    21781 acttctcgaa aatgtccagg ctctcgtggg cctccgcttc gctgatcacc agcggcggcg
    21841 ctacgcgaat cacgttgccg aaaacgccca cggaaccgat gagaagcccg ttctgcgccg
    21901 ccacgtcgat cagcttgcgc gtcagatccg gcgccggttc cttggtcttc ttgtctttga
    21961 ccagctccac gcccatcacc agtccccgcc cgcgcacatc gccgacgtaa ggagattttt
    22021 cctggatctg gagcagccgc gccttcatca cctcgcccat tcttagagcg ttctcgtgga
    22081 gtttttcgct ctccatgatg tcgacgactg caaccacggc ggcgcacgca accggattgc
    22141 ctcccatggt gctgctcatc tcacccgggc cgagcacacc gatgacctcg ccgcgggcgg
    22201 caacggcgga ggccggtaca ccgctgccga tgcccttccc gagcgccacc aggtccggcg
    22261 tcaggttctc ccattccatc gcgaacagct ttcccgttcg cccgaaggac gattgcactt
    22321 cgtcgagcgt aaacagcagc cctcgatcgc gaatccactg ttcgagtctg gggaaccagc
    22381 ccgggggtgc gaagatgaat ccggccgcgc ccaggtacgc ttccacgatg acgcctgcga
    22441 ggctcccggt gctgttggcc cgcacggcat cgtcgagata gtccaggcac agcatgtcgc
    22501 aggtctcggg agctgcgcgg aaggggcaac ggtagcaata cgggaacggt acgcggatgg
    22561 aaccgggcag tgtcgggcca tatccctttt tcggtccggc gagtcctccg gcgcccagcg
    22621 ccgcgtatgt gcggccgtgg aagccgccat gaaagctgat gatctcgaat ttgccgctct
    22681 tgcgtttcat caggcggata caggcttcgg tggcctcgct tcccgtgctc aggaagaagc
    22741 atttgtcgag gtgcgcgggc agaatctgga ccagcttttc ggcggcgttc acgcgcggct
    22801 cgttcaggca ctcgtaattg ttcatcagct tggcgcaggc agtctgcact gcctccacca
    22861 ggcgtggatg agaatgcccc acattggtca ccagcacacc ggaggtccag tcgataaacg
    22921 tgttgccgtc cacgtccgtg accactacgc cgttggcgtg gtcccaacat acacgcgcct
    22981 ggtagccggt gcattgcgct tctaccttgg cccagcgttc aaacagctcg cgcgatcgcg
    23041 gcccggggag ttggcagtga acccgggcgg cagcagttgt ttcggttccc atgattatga
    23101 ccttcctgtc tcgaccggca tgcgcggcgc actcacatta ttggcgacgt agcccggcgc
    23161 gaggcccgcc gcgaaacgct cgatttccaa cacgataatt tcccggatct tccacccggc
    23221 gtcttcggaa taccacgcca ggtgaggcgt caggatggca ttgggcaagc cgaagagagg
    23281 atagtccggc cgcggcggct ccgtctcata gacgtcgata ccggcgccgg caatcacgcc
    23341 gtttttcagt gcttccgcca gggcatcgac gtcgaccatg ccgccccgcg cggtgttcac
    23401 caggtaggcg gttggcttca tcagcgccag cgtttcgcgg ttcacaatgt agcgggtctc
    23461 cggcgccagc ggcgtgtgaa tcgtgatgaa atcggattcg cggaacaggg tctctttgtc
    23521 caccagttcg atatccaatt cgcgtttccg cttttcgctc agatagggat cgcagatgac
    23581 gaattcgaat ccgaagctct tcagtttctg atatacgcgg cttccaatgc ggccgcagcc
    23641 aaggatgccg agcttctgac cggccatgcg gaacatcgga atgacgtctt taaaatccca
    23701 gcggccgttc tgagacgatt cgtcaagaac cgtccggctt cttgggatgc ggcgcgcaca
    23761 ggcgaagatc agcgcgatgg cctgctcggc cacttcctcc acacagtaat cggggatgta
    23821 gcagagcggt atacccgcct cgtcgagcgc ggcacggtct acgttgtcat agcccacacc
    23881 gtggcgaatc accagcctgc accgtgtcca gctttcgatc agctcgcggg tgatgggaac
    23941 catgttcacc accaccacgt cggcatcgcg tgtggctgcg gccacgtcct caaagggcgc
    24001 aaacttgagt tgataaggtg caagctcgat cccctggccg ccgagttttt cggcttccca
    24061 attcagatcc ggttcaatgt aatcggtaac gacgactttc atctttcatc cttttccgct
    24121 gtggcgagcg caccggccag ggacaccgcg cgcccgcttc cggcggattc gtaaatcgct
    24181 tccacgactt cgagagcgcg caggccatcc tctcctgtaa tggcgggagg cttgccttct
    24241 cgaatggatt gcgcgagctc tttcaggaac ggcagagtga gaggtcccgc gtagccgggc
    24301 tgcgggtcga gctcgaaatc gatgcgccgg cgcgtggcgg gcgcaaaccc tggcgcatcg
    24361 ctgcacacga acaggttctc ccggatgcct tcaaacgacg ggctccagga aagcacaccg
    24421 gccgtgccgc gcaaggcgag gtacagatcg cggcccgccg gaaacgaatc cggcaccgtg
    24481 tagctgatat cgagcgccag caccgttccc cgcgaaaagg tgagcagcgc gaacgaattg
    24541 tcctcgatgt cgtattcctg gttgattttg acgtttttgc ccaggacctc tactacttcg
    24601 tcatcgagca tccagcggta aaggtcgatc cagtgaacgc cgaggttgta catgggcccg
    24661 ccgccgctca ttcgccggtt gagcatccag gcggcgttgc cttcgatgta gcgatgaaga
    24721 cggccggcgg cacagcggcc ctcgcccccg acgatccggc ccaacacgcc atcgtcgacg
    24781 tactgtttca tcgcacgagc caccggatga tagcgccaga catacggcgt cgataaaata
    24841 acgcccgctt tggcggcggc cgccatcatg caccgcagac cgtccgagcc cgccgccatg
    24901 ggcttttccg cgagtacgtg tacacccttt tccgcacagg cgatggccgc ttcgggacag
    24961 aggttgtgag gcaggaacaa caccgccaga tccagccgtt cccgatccag catctcgcgc
    25021 cagtccgcat agccgtggac cggaaagtcg cgggtgaaag attcgagcac cgcggggttg
    25081 ccgtccgccg ccgctactac tttcattcca gccacggcgc caaatacggg catgtagctt
    25141 ttgggatgca ggtgggaaag accgatgaaa ccgacagaca agccgctcat ggaaactcct
    25201 gttttcggcc tgtttccgcg tccgccggcc acaacaagcc gaaaattaag tttaaggtct
    25261 tgttatctca taggtcaacc acattttcaa attcgtgcat tgtgagtttc acatcgtgca
    25321 acaaatggtg cgcaaacgcc gcatcgcgct accgggtgcg ccggttgtaa aatcacgagt
    25381 acttccatga aacacgacga tcaggctaat gacccgcgca cggcctccgg acgccgccag
    25441 atgctggcgc gctcggcggc gttccttgcg gcgccaggca tgctggcggc gggatcgacg
    25501 caaccggcgc cggccccgca aaaaccggta cgcccgcgat ccacaccgcg ccgttatgag
    25561 atgaagaagt ccatcaacct gtgggcgttc ccctatccgg gccaatggag tctgaaagac
    25621 tgcctgcaga tttcaaaaga cgcaggcttc gacgccgtcg agctgaactt cgatctgacg
    25681 ggcgagttct cggccgaatc atccgacgcc cggatcaagg agattgggga gttggcgcgc
    25741 aaagtgggca tcggcatcag cggtatttgc tccttcctgt tctggcccta tccgctgacg
    25801 gcgaacgatc cggagaagcg gaagaaggcg atggcgttag gcgcgcgcat gatcgaagcg
    25861 gcgtctctgc tgggaaccga gaacctgctg gtggtgcctg gagccgttta cgcatcgtgg
    25921 gtggaggggt tcgaaccggt gccgaacgac gtctgcgacc ggcgcgcgcg cgaggcgatc
    25981 cgcgcgatga ttcccctcgc cgaaaaagcg ggggtttatc tgaacctcga aaacatcttc
    26041 agcagcgggt ttcttttcag cccgcaggag atgaacgaat tcgtggacag ctttcgcagc
    26101 aaccgggtgc gcatccactt cgataccggc aacatcatgc agtaccagtt tcccgagcat
    26161 tgggttccca tcctgggcaa gcgaatcaag aacattcatt tcaaggagtg ggacaagcgg
    26221 acgcacgaat tcaacctgca cacgttccgc cctctgctgg acggcaccac aaactggccc
    26281 gctgtcctgg ttgaactgga taagatcgga taccgcgact atctcacctt cgagtatttc
    26341 catccgttcc agcactatcc ggaagccctg atctaccaga cttcggacgc gctggactgg
    26401 atgctgggtc gcaaggggta acgcctttcc ctgaccggtc gcctacgctg cggcgccgag
    26461 cagccgttcc atgtaacgcg cgcttgcttc gaggtcttcc cgttcgcgcg cggcggcggc
    26521 atctttgccg gcggcgggcg ttggctgccg cggcgttcct gcgtccgcca gttggaggaa
    26581 ccgggcgaat tcccacgcgg gcatccgcct gtagccgtcc caaaactccg gcttggcgaa
    26641 cgcgaagatg cgcggctgcg tcacgatgat ttcaagggac aatgccttgc ccggacagag
    26701 cgcgaggtac ttgcgcacgt actcgtcgat cttgacattc ccctcgccca tacgcaccca
    26761 ctgcacagcc gcgccctccg gcacgcgcca gacggcgcta tcgcggacgt gactggtgag
    26821 aacgtaagga tgcaggtgat cgagtgtcac gtgcggatcc tcgatcgtcc atagcgggtt
    26881 ccccgaatcg atacaggcgc cggtgaagtc tttgcccgcc tcttcgatca gcatcttcaa
    26941 ttcacggccc tgcatatcgc ccgcatggtt ttcaacggcg attttgatcc ccttcgactc
    27001 ggctcgcgat ttgacggagc gcagcacctt cgcggtgttt tcgatgtgac cctcgatagg
    27061 gatggagcct gtacggtcgg ccatagagcc gaggaacgcc cgcacgatag gagaaccgat
    27121 gatcgctgca gcttcgatca tgcgctcgag ttgctgctcc gccgtgccct gtgagggatc
    27181 gaataatttg gaggtggggc aaatggagcg catcccgatt tcggtctcga tcccgagttc
    27241 ttcggcccgt gcgcgcacct tacgcaaatg agaaggatcg agtcctccga ggaaacggat
    27301 ctcggaaaaa tggactactt tggcgttcag cttcgcgcaa taagcgaggt attcaaaggc
    27361 ggtccaacct tgtgagcgca ggctgaagag gtccacgccc cgtttcacgt tgggcgccgc
    27421 gggaaccggc ggcactgccg ccgcggaggc agccgcgaga aagcttctgc gacgcatccc
    27481 agtattatag gtggaacgct accctttcag gtggcgcgcg agccagacaa tatactcggc
    27541 attgcgccgc tgcacttcct ggacgagttc ctcgggaagc tcatcgatct tctctttaaa
    27601 gaagcgacga tctccatccg gagcggcagt gtcgcgcgtg cccaccatgt tgccgaccca
    27661 gaaaacgaga aacaggtttc cgtccacgcg tccggcggcc gcattgccta aataataccc
    27721 gcggcccatg tccaggtatt ttgcggccag ctcgctctcg gcgccgaaca cgccttgtcc
    27781 ggcgaaccgg ttgcagcggt cggccatgcg ttcgtacaac tggcgccacc ggcttcccgc
    27841 cggctgttcc gagagaacat cgaacaactg taactgtagc tcgccggaga ttttgtaatt
    27901 cactctgccc gcggtgctac gcccgatgtg ttcgagcacc tcggcctgtt ttccactccc
    27961 cgaatggaca ctgagagtgc cgttgaaacg gcgggctacg gcggccagca ggcttacccg
    28021 cgcggcgagc tcttccagcg gctttccgcc gtattcgccg atcacgcggg gcaacaactc
    28081 cggccacatc ttgtgttgga cgtaatcctc caggccgacg ccgctttccg gacccgtctt
    28141 gaacgccatc ggataggcct gccgcttttt gaaaccgaga ttcggcggaa cgagttgagc
    28201 tgcccgtcca cgggtcttca accagtgcat gtaaaagatc agctcttgcg gtgtagtcag
    28261 cgtgtccgct tcatccagag acggctcgaa atcaaaagtc cgccgggccg gctcctgttc
    28321 tttcacctgt tggatgtagt cgtaaagctc ttcgttcagc ttcaagctgc gcccgaattt
    28381 cacggcgagc ctgacgatct cttcgcgccg cagaacgtaa gaagtttcgc cggtatcgaa
    28441 ggtgcccgcg aactcctgtt cgatccaact gcgctcgccg gcatcgaaga tctgctcata
    28501 ccgctcccgc acgaccgaat ccggccaggg gttgggatgc cgtgtatcgg cctgcaactc
    28561 gaaaagccgc gatgtatcag tggtgaactt ggtgtagccg gaagcgtgcc gaatcgcctc
    28621 tttccccacc tcgacggact gtgcgatttg cgagtcgtta tcgccggtga tgatgatgtg
    28681 atcggcttcc gcgttgtagc cgtgtctgaa tccgcagcgg atggcggccc acagcccggc
    28741 atgatagaga tgccggatag aaacgcgcgt gtgcccggct gacaccgggt tttcgccatc
    28801 tctgttcgcc agcgcttcct cggttgtcat ctcccgcgtg gcggatagct gcacggtgga
    28861 cgccaggttg atacctgtct catcgaggat cttctgaaat gcctggaacg ccgcggggag
    28921 gctgatctcc ggatggcggt tgccgacggc tatggcaggc tgtgcaccct gagggcgcgg
    28981 cagaagcgag cggtccagct caagcacgaa acggcgtatc tgttcgtgat ccgtagaggc
    29041 aaacggaatg gcgttcacca gcatttcgcg ctctccgccc ggacgcacgc gtccgatgga
    29101 aaccgcattg gagaacaatg gagcggcggg cgacgatgcg ggcgtaacgt gtatcagcag
    29161 gggcactctc cgcgtttccg ttaacgcatc aatggcaagg taatacgtcg aaggatgggc
    29221 gggatccatt tcgagtgagc cgggataagc gcggatggtg tccaaattca gttccgcgat
    29281 tcgggtggct atcagggcgc gggattcgcc ggtcggctcc ctgccatcta tttgttccag
    29341 gatcacctgg cacagcgcag cagcttcact ggcctttagc ggtaattcgg tcgggacggt
    29401 tgacaatctg tctcctataa tttgagagca ctgagatact ggcggaactc ttcgcccaaa
    29461 tcgtagcgct tcagtgcaaa ctccacggtt gctttcaaga agccgagctt atcacccgcg
    29521 tcgtatcgcg tgccttcaaa acgtaagccg taaatcggcc ggcttcgcaa caggtgtttc
    29581 agcgcatcgg tgagctgcaa ctccccgccg gatccgggct cgatcgattc aatggagtcg
    29641 aagatctcgg gcgtgagcac ataccggcct atgatcgcca gatttgatgg cgcctcgtcg
    29701 ggcctgggtt tctccaccat gttgcagatg cggaacaggc ggtctcgcgc tccgttgtgc
    29761 gcgatgggtt cggcgtccac cacgccgtag gacgaaatga tgtccttcgg cacttccatc
    29821 agtgcaacta ccgaggcgcc gtagaactca tagatatcga gcagttgcct catacagggc
    29881 acttcggcgt ctatgacgtc gtccgacagc accactccga acggctccgg cccgataagt
    29941 tcccgcgcgc gcaataccgc atggccaagc cccatcgcct ctttctggcg cacataggcg
    30001 acgttgatca tgtcggagat gctgcggatg gtattcagca gatctttctt gttgcgcgat
    30061 tcgagcaggt gctccatctc gaagctgaca tcgaaatggt cctctatcgc tgttttccca
    30121 cgacccgtga tgatgatgat gttctgaatt ccggaatgaa tggcctcttc tacgccatac
    30181 tgtatcagcg gcttatccac cagcggcagc atttcttttg gttgtgcttt ggtcgccgga
    30241 aggaagcggg tgccgaggcc ggctgccgga aagaccgcct tgcgtacttt cggaagcatc
    30301 ttatccattg taaacacaag agaaaattga cagcagaaat tgcgtgcccg ggaccggcgg
    30361 cggttgcctg attcagtcct cgagaataac gtacgcaagc gcctgctgcg ccgtatgggt
    30421 gagggagagc gatacacggc ggatgccaag cgcggcggcg aattcggccg ctactccgtg
    30481 aaattgcaga gtgggcctgc cggaccgttc gttcaccact tcaaaatcct gccagcggac
    30541 accgtgcctc caccctgtgc cgatggcctt catgcccgcc tctttagctg cgaagcgggc
    30601 agcgtatcgt tcgaaccggt tggcctttct ttccacgtaa gcgatctcgc cgggggtgaa
    30661 cacgcgctcg atgaaccgcg ccccgaaccg ttcgatactt tgacggatcc ggtgaacttc
    30721 gacgagatcg acaccggtgc cggcaatcac aactcggctc cggacgccgt gatggtcagc
    30781 cgcccgtgct ttacaccctt cgtactgatg agagcgtcgg caatcttctt tacgtccttc
    30841 gccctacctt tcaccacgag cacctcgagg cagttatcgt ggtccaggtg aacgtgcatc
    30901 gtggaaagaa tcagatgata gtgatcgtgc tgtaagtcgg tgagcttgtc gctgagcaaa
    30961 cgcacgtggt ggtcgtaaac cagagtcacc gtgcccacca cgggactttc cggcccctcc
    31021 catgccttct caacaagctc gtcgcggatc aggtcccgga atgcttccga tcggtttgta
    31081 tagccccggt ttgcgatcaa ctcatcaaat ttcgcgagca gatcggaatc gatcgccacg
    31141 ccgatccggc tcaattcgct catcttgtct ccagggaaat tataaccgtt cactcaaaac
    31201 gggttgtgcg aagcccgcgg gatccaggtg tagtatcatt tgggcatagg ctga
//
LOCUS       DASTMK010000268         9931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_236566, whole genome shotgun sequence.
ACCESSION   DASTMK010000268 DASTMK010000000
VERSION     DASTMK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236566"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VFQ79_11710"
     CDS             complement(<1..1466)
                     /locus_tag="VFQ79_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU0140376.1"
                     /translation="MNRAKVTIDGNEAAAYVAHKINEVIAIYPITPSSPMGEWSDQWS
                     SEGKPNIWGTIPTVVEMQSEGGAAGALHGALQGGTLATTFTSSQGLLLMIPNMYKIAG
                     ELTPTVIHVAARAVAAQALSIFGDHQDVMAVRQTGFAALASNSVQEVMDLALISQAAS
                     LESRVPFVHFFDGFRTSHEVAKVEQLTVEDMKAMIDDDLVRAHRERALSPDRPILRGT
                     AQNPDVYFQARETVNPFYLACPTIVQNVMDKFAKLVGRQYRLFEYVGAPDAERVIVLM
                     GSGAEAAEEAVDYLVAQGEKVGVLKVRLYRPFSVEHFLETLPATVKSIGVLDRTKEPG
                     AIGEPLYLDIVTGLSEAAAAGETRFASMPRIIGGRYGLSSKEFTPAMVKGAFDELAKD
                     RLKNHFTLGIQDDVSLTSLDYDPEFSTEHPKTVRALFYGLGADGTVGANKNSIKIIGE
                     ATDLHAQGYFVYDSKKSGAVTVSHLRFGPRPIRSTYLIG"
     gene            1745..2494
                     /locus_tag="VFQ79_11715"
     CDS             1745..2494
                     /locus_tag="VFQ79_11715"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU0140377.1"
                     /translation="MRKPNRTGAAAHRVVYEVREFCSSHADPKRAERYTRFFVEGYDA
                     YGVDTNAPEWGRQRQAWVGRLREAGPAAFLDAGDALVRSGKYEEASFAILFAADLTEY
                     HTPKAFKRIEAWFDGGIRNWAHTDILAGEVLSAFLLQGKVALEDLERWRDSPFKFQRR
                     AVPVTLIKLLKGNSDYSRLLAAIEPLMMDSEKVVQQGTGWFLRECWKREPKLTEEFLM
                     KYKDSSPRIIFQYATEKMAPKDRQRFRRAHK"
     gene            complement(2517..4040)
                     /locus_tag="VFQ79_11720"
     CDS             complement(2517..4040)
                     /locus_tag="VFQ79_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0140378.1"
                     /translation="MRVLLTNVTLATRTGTEIVTRDLALGLHRAGHDVCVFSPDPGGV
                     ADEIAGAGVPVVNNLHAVPFHPQIIHGHHHVETTLALLHFRSVPAIFVCHDRLVWHDS
                     PPKLSGVRRYVAVDHNCLERLINEAGIPAERTRVILNAVDLERFVPRKPLPPKPARAL
                     VFSNYATNGTDLNLLQSVCEDAGIGLAVTGKGVGVASLAPEQVLGDYDLVFAKARCAL
                     EALAAGCAVILRDWQGLGPMVTSDQVQDLREWNFGMRCLQAPLTAKALQREITRYDRH
                     DATEVSAYIRSHASLDAAVSQYVSLYKEVLAEAPHTPVSVSGVVTSLARGVGVLESVL
                     RGAGEIFAMRPLPRTTASRIRLRVPRKPPEFAAGSVTEIPLEIDNRSSEVLASLPPFP
                     VNVAYHWVAPRSRSVIVYDGGRTELSAPVRPRTRHSQNARVLAPKRPGRYVLLLTLVQ
                     EFQFWFDAERNGTAFKMEVMVRARDGGGPGELTLAEVVRGRVRGLYVTVRSRIASFF"
     gene            complement(4053..5294)
                     /locus_tag="VFQ79_11725"
     CDS             complement(4053..5294)
                     /locus_tag="VFQ79_11725"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140379.1"
                     /translation="MNRRDFLKVPAAAAFPYIARPTVLGANDRVTLGFVGVGNRARWM
                     LKNEDFGFGRVVAMADIWPQSFAETIKVRPDASAWTQYNDYRRMFDKEKLDAVFVETP
                     THPRVLTSIHAIQAGLDVYAEKPLTLTVEEGRVLTKFVRRYNRVLQTGTQQRSMPINR
                     YANSLVSSGKIGRIKEVIACNFLAPRRWQPLPEQPVPEGLDWDRWCDQTELRPYHPEL
                     HRGWSKWWDYDGGGQSWGVTGWGTHSLDQVQAALGMDDTGPVEMWPEEPGPEGKVTMR
                     YDNGTLLKLEQPIIKDHQQLGAIFIGTKGRIQIIRGDFHPDPPELKKGAPDVLPEGPG
                     EVTPHISNFIECVKSRKRPTADVEIAHRATTVCHLVNITRELGRKLRWDPKAERFRDD
                     EEANALLSRPRRKGYELPNPG"
     gene            5366..6685
                     /locus_tag="VFQ79_11730"
     CDS             5366..6685
                     /locus_tag="VFQ79_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140380.1"
                     /translation="MNRRYFLLGAAAAPPLARASTLASPNDTVRVACVGFHGQGRAHL
                     RAYPRLQNVEIAAVCDIDQSVLEQGAKQVEDATKKRPATFTDLRKLLEDKSIDAISIA
                     TPNHLHALQTIWGCQAGKDVYVEKPCTHNIFEARQIVAAARKYNRMVQHGTGARSSSS
                     VREAAQKLQEGVIGDLYMARGLCFKWRDTIGRAPVEPVPAGVDYDLWLGPAPKREFTK
                     NRFHYNFHWFWDYGNGDFGNQGAHQIDVARWLLGVKYPTRISAMGGHFMFDDDQETPN
                     TMVATFEFNHMSKKKMLVFEVRHWISNHEAGIGEPRPGATGPADPNTIGDVIYGSKGY
                     LVIGGGYKTFLGKEQEPGPASSGRGGGDNFANFIGAVRSRKTSDLNAEIEEGAISTVL
                     IHLANISYRLGRTLEFDPATTTCKNDAEANRMMTRQYRAPFVVPKQV"
     gene            6698..8014
                     /locus_tag="VFQ79_11735"
     CDS             6698..8014
                     /locus_tag="VFQ79_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140381.1"
                     /translation="MDRRYFLVGSFASVTAARAASFQSANNRVRIACVGFNNQGRAHI
                     DAYTKMPDVEVAALCDTDDAVLEKGCGMVEAAGRKRPAVYTDIRKLLEDKSIDAVSIA
                     TPNHQHTLQAVWACQAGKDVYVEKPCAHNVFEARQIVAAARKYNRVMQHGTGASAILH
                     ETKEKLRGGVIGDLHMARALCFKMRNTIGRAPVEPVPPGVHYDLWLGPAPKRAFTKNR
                     YHYNFHWFWDYGNGDIGNQGIHQLHAARWALGVKYPAKVSAVGGHFLFDDDQETPNTL
                     SATFEYNDSGKKRMLVFEVRHWFTNSEADLASKSDAFSGPAAGATQTVGNIFYGRDGY
                     MVLGNGFKTFLGKEQAPGPVSTSTGGDPYRNFIDVMRSRKRSDLLAEIEEGAISTVSV
                     HLANISYRLGRSLQFDANTMRCVGDEQANAMLTRAYRAPFVVPKIV"
     gene            8016..8738
                     /locus_tag="VFQ79_11740"
     CDS             8016..8738
                     /locus_tag="VFQ79_11740"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEU0140382.1"
                     /translation="MRIAILGVLGMCAAAASADELPRYKIQRASSPIVIDAKLDDPAW
                     RNAPAAGKFHLVGGKGEAAQQTVVRLLWDDENLYAGYHCDDRHISAYVNERHGPVSND
                     DCIEIFLSPNPEKVRNYYTFEINAIGAMLNRCRTDWWKGPATWEPEGVRYRTSFHGLP
                     KKDEQPSDNHWVVELAIPLRNFSHDASHTPPRPGDVWRLNLNRTGGRTNRESSTWSPL
                     GENVKTFHTPEAFGWVEFTGGR"
     gene            complement(8722..>9931)
                     /locus_tag="VFQ79_11745"
     CDS             complement(8722..>9931)
                     /locus_tag="VFQ79_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140383.1"
                     /translation="NDWRMLDVETGKPVYLPAYNAEQFCMNNAEFRQAYGKYVRQLVA
                     ETGIDGLMSDDGIFYAGWRACACEHCRDRFQKEYGRRLPPVADTSFWGNRGSEAFADW
                     IEMRFRTAGDYLAEIKTALPEGFPFLTCCSSSDGYTMPAVGMSYQDFIRSCNLVLLEM
                     AGSTPSIKGTWDTRIPSQLLHLGIARDHRVPCLSLGYGFFPDTAFFVWALNKFLGSDS
                     WFSTLKGRLNAGEAQLAALADDSELVEEGYRWEKEHPRLFTGDVDTDVAVFFSRATRD
                     YYGQCAEDYVNDYYASCLELLRREIPYEVAAGGTVAARPDHVRACDMRAEHLPPEFVI
                     AHAQPACGIGDGFFANCGGPVILPGVGDGTVAHRARVKHTPALFGGKRGGQQQAEGER
                     VSHNILTGRR"
BASE COUNT         2037 a         3078 c         2920 g         1896 t
ORIGIN      
        1 ccgatcagat aggtcgagcg gatcggccgc ggaccgaagc gcaggtgcga caccgtcacc
       61 gccccggact tcttggagtc gtagacgaaa tagccctgcg cgtgcaggtc ggtggcctcg
      121 ccgatgatct tgatggagtt cttgttggcg cctaccgttc catccgcacc aagcccgtag
      181 aacaaggccc ggaccgtctt cggatgttct gtcgaaaact cggggtcata gtcgaggctg
      241 gtgaggctga catcgtcctg aatgccaagc gtgaagtgat tcttcagacg atctttcgcg
      301 agctcatcga aggcgccctt taccatggcg ggcgtgaatt ccttggagga aagtccatac
      361 cggccgccga tgatgcgggg catggaggcg aacctggttt cacccgcggc tgcggcttcg
      421 ctcaggccgg taacaatgtc cagatagagc ggctctccaa tggcgcccgg ttccttggtg
      481 cggtcgagaa caccgatact cttcaccgtg gcagggagcg tttcgagaaa atgctccacc
      541 gaaaacggcc ggtacagacg gaccttcagc acgccgactt tttcgccttg tgccaccagg
      601 tagtcaacag cctcctcggc tgcctcggcg cctgaaccca tcaatacaat gacgcgctcc
      661 gcgtccggag ccccaacata ttcaaagagc cggtactggc ggccaaccag ctttgcaaac
      721 ttgtccatta cgttctggac aatcgtcggg caggcaagat agaacgggtt caccgtctcc
      781 cgcgcctgga agtacacatc ggggttttgc gcggtaccgc gcaggatggg acggtcgggc
      841 gacagagcac gctcgcgatg cgcgcggacc agatcgtcgt cgatcatggc cttcatgtct
      901 tccaccgtca actgttccac cttcgcgacc tcgtgcgagg tgcgaaagcc gtcgaagaag
      961 tggacgaacg gaactcgcga ttcaagggac gctgcctggg agatcagcgc caaatccatc
     1021 acttcctgca ccgagttgga agccagcgcg gcaaaccccg tctggcgtac ggccatcaca
     1081 tcctggtggt ctccaaaaat agagagggcc tgcgcggcta cggcgcgggc ggccacatga
     1141 atgacggtgg gcgtcaactc cccggcgatc ttatacatat tcgggatcat gagaagcagg
     1201 ccctgggaag acgtaaacgt cgtcgccaga gttccgccct gcaacgcgcc atggagcgcg
     1261 ccggcggccc caccttcgct ttgcatctcg accacggtgg ggatggtccc ccaaatgttc
     1321 ggcttgcctt ccgatgacca ctgatccgac cattcgccca tcggcgaaga cggcgtgatt
     1381 gggtagatag ctatgacttc gttgatctta tgagctacgt aagccgccgc ttcattaccg
     1441 tcaatggtta cctttgctct attcatttgt accttcaggt atgcattttt gagtccagcg
     1501 ggattccttc ctattataag accttttagt ctgcttctgg ggctgtaagc gccggaaaat
     1561 acagggcgcg ctgtctggaa agaaaaatat gccgccattt cgcccgctgt gggatttcac
     1621 gcatccgcaa gccgctcgac aggcttcccc caacctgcct ggggcagata gcgcaggttt
     1681 caaggtgcct aaaaaaaaca ctggcgccgg tattcggttt tgagtacagt tgatccgaaa
     1741 caccatgcga aagccaaacc ggaccggcgc tgccgcccac cgtgtcgtgt acgaggttcg
     1801 ggaattctgt tcctcccatg cggatcccaa gcgggccgag cgctacaccc ggttcttcgt
     1861 tgaaggctat gacgcctacg gagtggacac gaacgccccg gaatggggac ggcagcggca
     1921 ggcctgggtc gggagattac gcgaagcggg cccggcggct tttctggatg cgggcgatgc
     1981 gctggtacgc agcggcaagt acgaagaggc ctcgttcgcc attctgtttg cggcggacct
     2041 gacggagtat cacacgccca aggctttcaa acgcatcgaa gcatggtttg acggcggcat
     2101 tcggaactgg gcgcataccg acatccttgc cggagaggtg ctgagcgcgt tcctgttgca
     2161 gggtaaagtg gcgctcgaag acctcgaacg gtggcgcgac tcgcccttca agttccagcg
     2221 ccgcgccgtc ccggtcacgc tgataaagct ccttaagggc aattcggact attcccgcct
     2281 gttggcggcg attgaaccgt taatgatgga ttccgagaag gtggtgcaac aaggcaccgg
     2341 atggtttctg cgcgagtgtt ggaaacgcga acccaagctg accgaagaat ttttgatgaa
     2401 gtacaaagac tcctcgcccc gtatcatctt ccagtacgcc accgagaaga tggccccgaa
     2461 agatcgccag cggtttcgcc gggcacataa gtaatgggaa cagatctgtt tgggactcaa
     2521 aagaaactgg cgattcgaga acgcaccgtc acgtacaatc cgcgcactcg gccgcgaacc
     2581 acctccgcaa gcgtcagctc gccaggtccg ccgccatctc gtgcccggac catcacctcc
     2641 attttgaatg ctgtgccatt gcgttcggcg tcgaaccaga attgaaattc ctgcacgagg
     2701 gtaagcagca gtacgtacct gccgggccgc tttggagcca gcacgcgcgc gttctgcgag
     2761 tgccgggtgc ggggccgcac tggggcgctc agttctgtcc gtccgccatc gtacacaatc
     2821 actgatcgcg agcgtggggc cacccagtga taggcgacgt ttacaggaaa cggcggcaga
     2881 ctggcgagca cttcggacga gcggttatcg atctccaggg ggatctccgt cacgctccct
     2941 gccgcgaact ccggaggctt tcttggcaca cggaggcgga tgcgcgaagc ggttgtccgg
     3001 ggtaggggcc gcattgcgaa gatctcgccc gctccacgga gcacagattc gaggacgccc
     3061 acaccacgcg ccagggacgt caccacaccg ctcaccgaga ccggcgtgtg cggcgcctcc
     3121 gccaagacct ccttgtagag gctgacatac tgcgagacgg cggcatcgag cgaggcgtgc
     3181 gagcgaatgt aggcggacac ttctgtggcg tcatggcggt catagcgagt gatctcccgt
     3241 tgtagagcct tcgccgtcaa cggcgcttgc aggcagcgca taccgaagtt ccattcgcga
     3301 agatcctgaa cctggtcact agtgaccatg ggccccagcc cctgccagtc gcggagaatc
     3361 acagcacatc cggccgccag ggcctcgagg gcacagcgtg ccttggcaaa taccaggtcg
     3421 tagtcgccga gtacctgctc cggcgcaagc gatgccacac ccacaccttt tcctgttaca
     3481 gccaagccga ttcccgcatc ctcacagaca ctctgcaaca ggttgagatc cgttccgttt
     3541 gtggcgtaat tgctgaagac gagggctcgc gcgggctttg gcggcagcgg ctttctgggg
     3601 acaaaccgtt caaggtccac agcattcagg atgacgcgcg ttcgctccgc gggaattccc
     3661 gcctcattta tcaagcgctc caggcaattg tgatccacgg caacgtagcg gcggacccca
     3721 ctcagcttcg gcggactatc gtgccagacc agacggtcat ggcagacgaa gatcgcgggc
     3781 acacttcgaa agtgcagcag tgccagcgtg gtttcgacgt gatgatggcc atggatgatt
     3841 tgaggatgga acgggacggc gtgtaagttg tttacgacag gcactccagc gccggcgatc
     3901 tcatctgcca cgccgccggg atcgggcgag aacacacaaa cgtcgtggcc ggcacgatgc
     3961 aggcccagcg cgagatcccg cgtcacgatc tcggtacccg tgcgtgtcgc gagcgtcaca
     4021 ttagtcagaa gaactctcat gccagccggc ccttaccccg gattcggcag ctcgtagcct
     4081 ttgcggcgcg ggcgtgacag caacgcgttc gcttcctcgt cgtcacgaaa tctttccgcc
     4141 ttcgggtccc agcgcagctt gcggcccaac tcccgggtga tgttcaccag gtggcagacc
     4201 gtcgtggcgc ggtgtgcgat ctccacatct gcggtgggcc gcttgcggct cttaacgcac
     4261 tcgatgaaat tactgatgtg cggcgtgact tcgccgggtc cctccggaag cacgtccggc
     4321 gcccctttct tcagctcggg cgggtcggga tggaaatcgc cgcgtataat ctggatgcgc
     4381 cccttggttc caataaagat cgctcccaac tgctggtgat ctttgatgat gggttgttcc
     4441 agcttgagca acgtgccgtt gtcgtagcgc atggtgacct tgccttccgg gccaggctct
     4501 tcaggccaca tttccaccgg accggtgtcg tccatcccta gtgccgcctg cacctggtcc
     4561 agcgagtggg ttccccatcc ggtaacgccc caggactgcc ctcccccatc gtagtcccac
     4621 catttcgacc acccgcgatg cagttcggga tgatacggcc gcagttccgt ctgatcgcac
     4681 catctatccc aatcgaggcc ttcgggaaca ggttgctccg gcagcggttg ccagcgccgc
     4741 ggcgcgagga agttgcaggc gatcacttct ttgatcctgc cgattttccc gctgctcacc
     4801 agcgaattcg catagcggtt aatgggcatg gagcgctgct gcgttcccgt ctgcaacacg
     4861 cggttgtagc gtcgcacgaa cttcgtcagt acccggccct cctccaccgt cagcgtcagc
     4921 ggcttttccg catatacatc caggccggcc tggatggcat gaatcgaagt cagaacgcgc
     4981 gggtgtgtcg gggtctcaac aaacacggcg tccagcttct ccttgtcgaa catccgccga
     5041 tagtcgttgt attgagtcca ggcggacgca tccggacgca ctttgatggt ctcagcgaag
     5101 gactgcggcc agatgtccgc catcgccact acgcggccga atccgaaatc ctcgttcttc
     5161 agcatccacc gcgcgcggtt gcccacgcca acgaatccga gcgttacgcg atcgtttgcg
     5221 cccagcactg ttggcctggc aatatacggg aacgccgccg cggccggcac tttcaggaaa
     5281 tctcgtctat tcacttttca cgcctccacg ggcggagtag ctattctatc gcgtgcaatt
     5341 gaggaataat attggagcta tgcacatgaa ccgtaggtat ttccttctgg gagcggcggc
     5401 ggcgcctccg cttgcccgcg ccagcacact ggccagcccg aacgataccg tgcgcgtcgc
     5461 ctgcgtgggc ttccacggcc agggcagagc gcacctgcgc gcctatccaa gactgcaaaa
     5521 cgtcgagatt gccgccgtct gcgatatcga ccaatccgtg ctcgagcagg gcgcgaaaca
     5581 agtggaagac gctaccaaga agcgtcccgc tactttcacc gatctgcgaa aactgcttga
     5641 ggacaaatcg atcgacgcga tttcgatcgc cacgcccaac catctgcatg cgctgcaaac
     5701 catttggggt tgccaggccg gcaaagacgt ctacgtggaa aaaccgtgca cgcataatat
     5761 cttcgaagcc cggcagattg tcgcggcggc gcgaaaatac aaccgcatgg tgcagcacgg
     5821 caccggcgcc cgatcctcaa gctccgtgcg cgaggcggcg cagaagttgc aggagggagt
     5881 gatcggcgac ctctacatgg cacgcgggct ttgtttcaag tggcgcgata ccatcgggcg
     5941 cgccccggtg gaaccggtcc ccgcgggggt tgattacgat ctctggctcg gccccgcacc
     6001 caagcgcgag tttacgaaga accgattcca ttacaacttc cactggttct gggattatgg
     6061 caacggcgac ttcgggaatc agggcgcaca ccagatcgac gtcgcgcgat ggctgctggg
     6121 cgtgaaatac cccacgcgga tcagcgccat gggcggccac ttcatgttcg acgacgacca
     6181 ggagacgccg aataccatgg tcgccacctt cgagttcaat catatgagca agaagaaaat
     6241 gctcgtcttc gaagtgcgcc attggatcag caaccatgag gctggaatcg gcgagccgag
     6301 gcccggcgct acaggccccg cggacccgaa cacgatcggc gacgtcatct acggctcaaa
     6361 gggctacctg gtgatcggcg gcggttacaa gacgttcctg ggcaaagagc aggagccggg
     6421 gccggccagt tccgggcgcg gcggcggtga caacttcgcc aattttatcg gcgcggtgcg
     6481 aagccggaaa acgtcagacc tgaatgccga gatcgaagaa ggcgctattt ccaccgtgct
     6541 gattcacctg gccaacatct cttaccggtt gggtagaacg ctcgaattcg atcccgccac
     6601 gacgacgtgc aagaacgacg ccgaagccaa tcgcatgatg acccggcagt atcgcgcgcc
     6661 ttttgtagtg ccgaagcaag tataagaggg ttccggaatg gacagaaggt atttcctcgt
     6721 tggttctttt gcgagcgtaa ccgctgcccg cgccgcttcc ttccaaagcg cgaacaacag
     6781 ggtacgtatc gcctgcgtcg ggttcaacaa ccagggccgg gcgcacatcg acgcttatac
     6841 gaaaatgccc gatgtcgagg tggcggcgct gtgcgatacg gacgacgcgg tcctcgaaaa
     6901 gggctgcggg atggtggaag cggccggcag aaaaaggccg gccgtttata ccgatatacg
     6961 aaaactgctc gaggacaagt ccatcgacgc cgtctccatc gccacaccga accaccagca
     7021 cacactgcaa gcggtatggg cgtgccaggc gggcaaagat gtttatgtcg agaagccgtg
     7081 cgcccataat gttttcgaag cgcgccagat tgttgccgca gcgcggaaat acaaccgcgt
     7141 gatgcagcac gggacgggtg cttcggccat tctgcatgag acaaaagaaa aactgcgcgg
     7201 aggcgtcatc ggcgacctcc acatggcgcg cgcattatgt ttcaagatgc ggaataccat
     7261 cggccgagct cccgtcgaac cggtgccgcc gggagtccat tacgatctct ggctggggcc
     7321 ggcgccaaaa cgtgcgttca cgaaaaaccg gtaccactac aactttcact ggttctggga
     7381 ctatggcaac ggtgatatcg gcaaccaggg aattcaccaa ttgcatgccg cccgctgggc
     7441 attgggagtg aagtatccag ccaaggtaag cgccgtcggc gggcatttcc tgttcgacga
     7501 cgatcaggaa acgccgaata cgctgtcagc gaccttcgaa tacaacgata gcgggaagaa
     7561 aaggatgctc gtgttcgagg tccgccactg gttcactaac agcgaggcgg acctcgcctc
     7621 gaaatccgac gccttctccg ggcccgccgc gggcgcgaca caaaccgtcg ggaatatttt
     7681 ttacggccgc gacgggtata tggtgctggg aaacggcttc aagacctttc tcggcaagga
     7741 gcaggctccc ggcccggtct ccacaagcac cggcggcgat ccctacagga acttcatcga
     7801 cgtgatgcgg agccgtaaac gttccgacct gctggcggaa atcgaagagg gcgcgatttc
     7861 aaccgtgtcc gttcacctgg cgaacatctc gtatcgtctt ggccgctcgc tccagttcga
     7921 cgcgaacacg atgcgttgcg tgggagacga gcaagccaac gcgatgttaa cccgggccta
     7981 ccgcgcaccg tttgttgtgc ccaagattgt ctgagatgag aatcgcgatt ctgggggttc
     8041 tcggcatgtg cgcggccgcg gcgtccgcgg acgagttgcc gcgatacaaa atccaacgag
     8101 cgtcatcccc catcgtgatc gatgcgaagc tcgacgaccc cgcgtggcgc aacgcgcccg
     8161 ctgccggcaa gtttcatctt gtcggtggaa aaggcgaggc agcgcagcaa accgtcgtac
     8221 gcctgctctg ggatgacgag aacctctatg ccggctacca ctgcgacgac cggcatattt
     8281 cggcttacgt caacgaacgg cacgggccgg tgtcgaacga cgattgcatc gaaatcttcc
     8341 tcagccccaa cccggaaaaa gtgaggaact attacacctt cgagatcaat gccatcggcg
     8401 cgatgctgaa ccgctgccgc acggactggt ggaagggtcc ggcgacgtgg gagcccgaag
     8461 gggtgcgcta ccgcacttcc ttccacggat tgccaaaaaa ggacgagcag ccttcagaca
     8521 accattgggt agtggaactc gccattccgc tccgcaactt ctcgcacgat gcctcgcaca
     8581 cacctcctcg tccgggggat gtgtggcgtc tgaatctgaa ccgcacggga ggaaggacga
     8641 acagggagag tagcacctgg tcgccgcttg gggagaatgt caaaaccttt cacacaccgg
     8701 aagcattcgg ttgggtggaa ttcaccggcg gccggtgagg atgttatgag acacacgctc
     8761 gccctctgcc tgctgctggc cgccccgctt gccgccgaaa agcgcgggag tgtgttttac
     8821 ccgcgcgcgg tgtgcgaccg tgccgtcgcc aacgcccggc aatatgactg ggccgccgca
     8881 attcgcgaaa aagccatcgc cgatgccgca cgctggctgc gcatgagcga tgacgaactc
     8941 tggcgggaga tgttcggccc gcatatcaca cgctcgcaca tggtctggtc gagcggccac
     9001 tgtcccgcct gccgccacct cgtacggaat ctcgcgccgc agcaactcga ggcacgaagc
     9061 atagtaatcg ttcacgtagt cttccgcgca ttgcccgtaa tagtcccgcg tcgcccgtga
     9121 gaagaaaacc gcaacgtccg tgtccacatc gcccgtgaac aaccgcggat gttccttctc
     9181 ccagcggtag ccttcttcca ccaactcgga atcgtccgcc agcgccgcga gttgcgcctc
     9241 tccggcgttc aaccttccct tcagcgtgct gaaccaactg tcagacccga ggaacttgtt
     9301 cagcgcccac acgaaaaacg ccgtgtcggg aaagaacccg tagcccaggc tcaggcaggg
     9361 cacgcgatgg tcccgcgcaa ttcccaggtg cagcagttgg ctcggaatcc gcgtgtccca
     9421 cgttcccttg atcgatggcg tcgaaccagc catctcgagc agcaccaggt tgcaactgcg
     9481 gatgaaatcc tgatagctca tcccgaccgc cggcatcgta tagccgtcgc tgctcgagca
     9541 acacgtgagg aagggaaatc cttccggcag cgccgtcttg atttctgcga ggtaatcgcc
     9601 ggctgttcga aaccgcatct cgatccaatc cgcaaacgcc tcgctcccgc ggttgcccca
     9661 gaagctcgtg tcggccaccg gcggcagccg ccgtccatac tccttttgga agcggtcccg
     9721 gcaatgctcg cacgcgcagg cccgccagcc cgcataaaag atgccgtcgt cgctcatcag
     9781 accgtcgatt cccgtctcgg cgaccaattg ccggacatac ttcccgtacg cctgccggaa
     9841 ttccgcgttg ttcatacaga actgctccgc gttgtaagcc ggcagataca cgggttttcc
     9901 ggtttccaca tccagcatcc gccagtcgtt c
//
LOCUS       DASTMK010000269         9782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_332872, whole genome shotgun sequence.
ACCESSION   DASTMK010000269 DASTMK010000000
VERSION     DASTMK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332872"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /locus_tag="VFQ79_11750"
     CDS             complement(<1..2087)
                     /locus_tag="VFQ79_11750"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0140384.1"
                     /translation="MTFVGRTIPATILVLFALCATAPAQTTFATITGTVTDASGATVP
                     DAKVTARHIATGIESAAVSNTEGVYTIPQLREGGYTVQAGKPGFREFVVHGIELVGRD
                     YRRVDVVLQVGSVETVVEVTGGATLIETETARISDTRNVMQLRDMPLNSRAIWAQLSL
                     VPNVLQASAGSTIRFSGSRSNQSYWSIDGTSMSDGVDETQIGPLANYVESFQEIRIDS
                     SNNTAEFGTIGQVTMVTKSGTNSFHGSLFDYYSTPWFRARNPFASSRPTGISHLPGGS
                     AGGPVYIPKVYDGRNRTFVFGSFETFRGSVTTQSFNPNVPLLPWRSGDFSGLSTPIID
                     PQTRAPFADNRIPANRINPAALAIQNRFYPEPNFGSAASTNQNYRADLTLPAMKQNYY
                     MIRGDHRFSDKDSVMGRYTLQDFKTDSFTGTLPTISPGYAMRKNHAATLAYTRTFSPA
                     IVNELRWGIATNNLPIFPPISGSEFVQEFGITGLAPDLPDVPGILNVSFAGLALTNIS
                     QGNFRNPGATNHVQNIQEHISIFRGRHSMKFGFNIARIEADNYGADANLFGNLQFSNR
                     YTGHPYADFLLGLPSTATRSFPPLRVDRRRWQYDFFYTDNFKITSKLSLDYGLRYEYH
                     PGWQEDNGYLSMFDIGTGKIVVQDGSIDKVSRVFPTNYVDIVEASSIGLPGNTLIRTD
                     RNNFAPRLGLAWRP"
     gene            2302..3291
                     /locus_tag="VFQ79_11755"
     CDS             2302..3291
                     /locus_tag="VFQ79_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0140385.1"
                     /translation="MSTDAALQSAVARLDQVRQEIAKVIVGQQDVVEGVLTCMLAGGH
                     VLLEGVPGLGKTTLLRTLARVLRLKYSRIQFTPDLMPADIVGSMVIETDDRGSKSLRF
                     QPGPIFAHLVLADEINRATPKTQSALLEAMQERTVTSGTSTHELEAPFLVMATQNPIE
                     MEGTYPLPEAQLDRFLMKILVDYPSREELNRIVDLTIQREDVHLQPQIDREGIMQVKS
                     VCRQVLVAPHVQGFAVDLVMATQPGSSQAHPLATKYIRYGSSPRGAQALVECGRVRAL
                     WHGRFHLSVEDIMAVAGSVLRHRIILNFDAHADGQSPDSVLTEIIQSVSAPAV"
     gene            3341..4225
                     /locus_tag="VFQ79_11760"
     CDS             3341..4225
                     /locus_tag="VFQ79_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0140386.1"
                     /translation="MSAAPLIDRAFLEKLERLTLRWRKSFPGLVGGHNTSRFPGPGQE
                     FFDHRHFHYGDDLRAVNWRAYLRLEKLFLKMFQVEPRVPVRVLLDTSGSMGTGEPSKF
                     VYARRLAAALTYVGLVRLDTIYLHPFSSTLGEGMVCSGGRHRFAPVVDFLTGLEARDA
                     SQYAPVARQFASTYVQRGLLVILSDFLDDAGCEKPLQFLADGGHELLLIHLWADEDRE
                     PPWVGELDLTDAETGEKLEISFDEGARREYTASFDSYARTLQELAVRTGGRYIGLSTA
                     VPFEEAIFGPLIHAGGVV"
     gene            4230..6185
                     /locus_tag="VFQ79_11765"
     CDS             4230..6185
                     /locus_tag="VFQ79_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEU0140387.1"
                     /translation="MSLLNLSLGEFFALFGALSGIVLTLYLLDRSRRRQVVATLRFWK
                     PAEHPPEARNRRRIQQPWSLLLQILSICLLLLAIAQLQFGSRERSARDHIVILDTSAW
                     MSARTGQITLADQSKALARAYVRSLPAEDRVMIVRADALPTPATPFESDRARIGQAIR
                     DTTPGSAALDLNEALEFTSKVMRTRATRPGEVVFAGAGRIARHDADASTSMPRIDNLR
                     VLPVTQVAENCGIRKIGLRRSMTADGVWQIFVAVRNYGRAPRQVSLGLRFGGAPAGSR
                     TLTLPPDSEQNITFEYKTRVAGWLEARLLDRDDFAADNRAVLELPAMPLLKVAVFSDE
                     PGLLRPVLASNPGVEARFLRTSQYDPGTDAQVIVLDRFRPARLPAAAGTLWIEPPSAG
                     SPVPVRTVVQNAPVVSWNSDHQLGAGLHTKDLRLGSAQVFATSGTDIPIASVETGPVI
                     VARPGTPKQVVAGFHPMRSGMRYELATPLLFANMLHWMAPEVFRRTEIMAGSVGSIAH
                     EFGSDVPAPDISLTGEGQEPIPFTIQGRQLRFFTLIPGIVRLKSPEGEAVYSLTLPEV
                     GETRWEPPKTALRGIPRQFAGLAPATDIWPWLAVLGGIGLLAEWLLFGRARRSNFFGR
                     APARITSLAARLPWKNRTRGRPVRRAS"
     gene            6182..8662
                     /locus_tag="VFQ79_11770"
     CDS             6182..8662
                     /locus_tag="VFQ79_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0140388.1"
                     /translation="MTFAHPWLLLLVPLPALWAAYSWRSTSRKGALALKALSLAAILL
                     ALTQPSVDVAGTRMSVSVLVDTSQSIGEAGMSRASSIASSIQSRRGRHHVEIVPFASG
                     TRALASGEKGSSLKLRRTAGAPGNGTDLENAIREALASAPAGLIPRLVLISDGRENQG
                     GVVRAMWQARELGVPVDTYAVEGKSKPGIVLESVSVPSFAFTGDRVPVELTLDSPRAA
                     AATVEVMAGGKVLGKSEVSLGAGRNQVRVYANLGAVGAIDFSGRVSSPDVGEVQFSQA
                     LTLRKPRVLYISQDPPGTDLHLMQALRAAEFDVQTAAGPAPRDLSEYQLVVFNNWDLD
                     GIAPNRKEDIEKFVKEGGGVLVIGGERNIYREKEGPEDPLERSLPAKLAPPRSPEGTC
                     VVLIVDKSSSMEGRKMELARLASIGVIENLRPTDMIGVLIFDNSFQWAVPIRRAQDRS
                     LIKRLVAGITPDGGTQIAPALSEAYRRIQPVQATFKHIVLLTDGISEEGDSVSLAKEA
                     GSQRVTISTVGLGQDVNRGYLEKVATFAKGKSYFLTDPSGLEQILLKDVMEHTGTTTV
                     EKLIRPAVVKNAEILDRVGIETAPPLRGYVRFTAKPSAETILSVERADPLLVRWQYGL
                     GRAAVFTSDAKSRWAEQWVSWNGYDRFWSNLFRDLLPHTQLGEAVVDYDRATRELIVD
                     YRLAPHVPEPEQIPEIYAMGPEGFQRPVEVKKVAAGTYRGRVATGHRNGLFRLRPLAE
                     SRAFPEIGYYREEQELRDYGSDSFLLQEIARFTGGRFNPDPRDVFDASGRSIETSLRL
                     WPGLLALALALNLAELILRKWRGIFQWR"
     gene            8707..>9782
                     /locus_tag="VFQ79_11775"
     CDS             8707..>9782
                     /locus_tag="VFQ79_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HEU0140389.1"
                     /translation="MKDFSEAPFLVIWETTQACDLACAHCRASAVECRHPLELTTEEA
                     FGLLREVREFGDPLMVFTGGDPLKRDDIFDLLQYSVQLGLRTTITPSATPLLTASAIE
                     RFKTCGVARMAVSLDGHDRALHDGFRGVEGSFDRTISALDHARAVGLDTQINTTVTRR
                     NLHVLHEIAARVAASGAKLWSVFFLITTGRATAGDDLTAEEYEQVFEFLYNLSKSAPF
                     DIKTTEAQHYRRYVAQRRKEEGGAVRSGREAPPSIIQRQAGINDGRGFVFISHTGDIY
                     PSGFLPVKGGNVRQERLGDIYRDSELFLTLRDSDLLEGKCGDCEYRNLCGGSRSRAYA
                     LTGNFMASDPRCVYEPAVKRERTR"
BASE COUNT         1808 a         2895 c         3120 g         1959 t
ORIGIN      
        1 gggcgccacg caaggcctaa tcggggcgcg aagttgttcc tgtcggtgcg aatcagcgtg
       61 tttcccggca ggccaatgct gctcgcctcc acgatgtcca cgtagttcgt tgggaaaacg
      121 cggctcacct tatcgatgga cccgtcctgt accacaatct tccccgtgcc gatgtcgaac
      181 atggagagat agccgttatc ttcctgccat ccggggtggt actcgtagcg gagcccgtaa
      241 tcgaggctga gcttggaagt aattttgaag ttgtcggtgt agaagaagtc gtactgccag
      301 cgcctgcggt ccacgcgcag cggcgggaaa gaccgggtcg ccgtggacgg caaccccagc
      361 aggaagtcgg cgtacggatg gccggtatag cggttcgaga actgcaggtt gccgaagagg
      421 ttggcgtcgg cgccgtagtt gtccgcttca atccgggcga tgttgaagcc gaacttcatg
      481 ctgtgacgcc cgcggaagat gctgatgtgc tcctggatgt tctgtacgtg gttcgtggcg
      541 ccggggttcc tgaagttgcc ctggctgatg ttcgtgagag cgagtcccgc gaagcttacg
      601 ttcaggattc caggcacgtc cggcaggtcg ggcgccagac cggtgattcc gaactcctgc
      661 acgaactcgc tgccgctgat gggcgggaat atcggcaggt tgtttgtggc gatgccccag
      721 cgaagttcgt tgactatcgc cggcgagaag gtccgggtat aggcaagggt cgcggcgtga
      781 ttcttgcgca tggcgtatcc cggagagatg gtgggcagcg taccggtaaa gctgtccgtc
      841 ttgaagtcct gcagcgtgta gcgccccatc accgagtctt tgtcggaaaa ccggtggtcg
      901 ccgcggatca tgtagtagtt ctgcttcatc gcgggaagtg tgaggtcggc gcggtaattc
      961 tggttggtag aggcagcgct gccgaagttc ggctccggat agaagcggtt ctggattgcc
     1021 agtgcggccg gattgatgcg gttcgcggga atgcgattgt ccgcaaacgg ggcgcgggtc
     1081 tgagggtcga tgatgggcgt cgaaaggccg gaaaaatcac cggaccgcca gggcagaagc
     1141 ggaacgttcg gattgaacga ctgggtggtg acgcttcccc ggaaggtctc gaacgatccg
     1201 aacacgaacg ttctgttgcg cccgtcataa accttgggga tgtaaacggg accgccggcg
     1261 ctgccgcccg gaaggtgaga gatgcccgtc ggccgtgaag acgcgaacgg gttgcgcgcc
     1321 cggaaccagg gcgtcgagta gtagtcgaac aagctgccgt ggaaggaatt cgtgcccgac
     1381 ttggtcacca tggtcacctg cccgatggtt ccgaactcgg cggtgttatt cgacgaatca
     1441 atgcggattt cctggaagga ttcaacgtag ttggccagag gtccgatctg cgtttcgtcc
     1501 acgccatcgc tcatactggt gccgtcgatg ctccaatagg actgattgga gcggctgccg
     1561 gagaagcgga tcgtcgagcc ggcggacgcc tgaagaacgt tcggaaccag cgacaattgc
     1621 gcccaaatcg cccgcgagtt caagggcatg tcgcggagtt gcatcacgtt gcgcgtgtcc
     1681 gagatgcgcg ccgtctcggt ttcgatgagc gtggcaccgc cggtaacctc gaccacggtt
     1741 tccacggaac ccacctgcag taccacgtca acacggcggt agtcgcgtcc caccaattca
     1801 atgccgtgga ccacgaactc tctgaacccg ggtttgccgg cttgtaccgt atagccgccc
     1861 tcccgcaatt gcggaatggt gtacacgcct tccgtgttgg ataccgccgc cgattcgatc
     1921 ccggtggcga tatgccgggc ggtgactttc gcatcgggaa cggtggcgcc actggcgtct
     1981 gtgacggtcc ccgtgatggt ggcaaaggtc gtttgcgcgg gcgctgtcgc gcaaagcgca
     2041 aacagcacca ggatggttgc aggtatggta cggcccacga atgtcatgtt tgaagctcct
     2101 gatttcacca aggccgtgca gggagaaagc ggcgcggcgt gctaccccga caccccggca
     2161 ctttcagttc tgcatcgttt agcataatga aacacaaacg aggggatgga cgcaattctt
     2221 tttctgtaaa atcacttcgc gaccgccaga ggtgttccgg aggccggtgt ccgccggccg
     2281 ttcctgctct agaatagtag tgtgtccacc gatgctgctc tccagtccgc ggtagccaga
     2341 ctggatcagg ttcgtcaaga aattgccaag gtcatcgtcg gtcagcagga tgttgtcgag
     2401 ggcgtgctta cctgcatgct ggccggcggg cacgtgctgc tcgaaggcgt acccggcctt
     2461 ggaaaaacca ccttgctgcg cacactggcg cgtgtgctgc ggctcaaata ctctcgtatc
     2521 caattcacac ccgacctgat gcccgccgat atcgtcggca gtatggtcat cgagacggat
     2581 gatcgcggct cgaagtctct tcgcttccag ccgggaccca tttttgctca tttagtgctc
     2641 gccgacgaaa tcaaccgggc caccccgaaa acgcaatcgg ccctgctcga agccatgcag
     2701 gagcgtacgg taaccagcgg aacctccacg catgaactgg aagctccgtt cctggtgatg
     2761 gccactcaaa accccatcga gatggagggc acgtatcctc ttccggaggc gcaactcgac
     2821 cggtttctga tgaagattct ggtggattat ccgagccgcg aagaactgaa ccgcattgta
     2881 gacctcacta ttcagcgcga agatgtccac ttgcagccgc agatcgaccg cgagggtatt
     2941 atgcaagtca aatcggtctg ccggcaggtg ctggtcgccc cgcatgtgca ggggttcgcc
     3001 gtggatctgg tgatggcaac gcagccggga agcagccagg cgcacccgct tgctaccaag
     3061 tacatccgct atggaagctc tccccgaggg gcgcaggcgc tggtcgaatg cgggcgcgtc
     3121 cgcgcactgt ggcatggccg ttttcacctc agcgtggaag acattatggc ggttgcggga
     3181 tctgtgctcc ggcaccgtat tattctgaat ttcgatgcac acgcggacgg gcagtcgcct
     3241 gattccgtgc tcaccgagat catccagagt gtcagcgcac ccgcggtttg aggcttgctc
     3301 gcggcttgta accacgtttc gccgggaagg agtttgaccg atgtccgccg ccccgctcat
     3361 tgacagagct ttcctcgaaa aactcgagcg tctgaccttg cgctggcgca agtcgttccc
     3421 gggacttgtc ggcgggcaca atacttcgcg cttcccgggc cccgggcagg agtttttcga
     3481 tcaccgccac tttcactacg gggacgacct gcgcgcggtg aactggagag cgtacctgcg
     3541 ccttgaaaag ctgtttctca agatgtttca ggtagagcct cgcgtgccgg ttcgcgtgct
     3601 gctcgatacc agcggatcga tgggaacggg tgagccctcg aagttcgttt acgcccgcag
     3661 actggcggcg gcccttactt atgtcggcct ggttcgcctg gacacgattt acctccatcc
     3721 attttcttcc acgctgggcg aaggcatggt ttgctcgggc gggcggcacc ggtttgcgcc
     3781 ggtggtcgat ttcctcaccg gactcgaagc cagggatgcc agccagtacg cacctgtggc
     3841 cagacaattt gcctccactt acgtgcaacg cggcttgctg gtgattctgt cggacttcct
     3901 cgacgatgcc ggttgtgaaa agccgctcca gtttctcgcc gatggtggac acgaactgct
     3961 gctgattcac ctctgggccg acgaagaccg ggagccgccc tgggttgggg aactcgacct
     4021 gaccgatgcg gagacgggcg agaagctcga gatcagcttc gacgaaggag cgcgccgcga
     4081 gtatacggcg tctttcgatt cgtacgcgcg aacgctccag gagcttgctg tccgcacagg
     4141 agggcgctat atcggattgt ccaccgccgt tccattcgag gaggcgatct tcggaccgct
     4201 gatacacgcc gggggagtgg tttgagacca tgtcgctgct gaacctcagt ctcggcgagt
     4261 ttttcgcact tttcggagcg ttatccggca tcgtgctgac gctctacctg ctcgaccggt
     4321 cacggcggcg gcaggtggtt gccactctcc gcttctggaa accggccgaa caccctcctg
     4381 aagcgcgcaa ccgccgtcgc atccagcagc cctggtcgct gttgttgcag atcctgagta
     4441 tctgcctgtt gctgctggct atcgcacagc ttcagttcgg ctcgcgcgag cgctccgcgc
     4501 gggatcacat cgtgattctg gatacttccg cgtggatgtc cgcgaggacc gggcagatta
     4561 cgctggccga ccagtcgaaa gccttggcca gagcttatgt acggtctctg cctgctgaag
     4621 accgggtcat gattgtcagg gccgatgcac tgccgacgcc cgctactccg tttgaatcgg
     4681 accgcgcgcg catcgggcag gccattcggg acacgactcc cggttccgcc gcactcgacc
     4741 tgaacgaggc cctcgagttc acctcgaagg tgatgcgaac acgcgcgacg cgtccgggtg
     4801 aggtcgtatt tgccggagcc gggcgcatcg cccggcacga tgccgatgcc agcacgagca
     4861 tgcctcgcat cgataacctt cgagtattgc ccgtcacgca ggttgcggaa aactgtggga
     4921 ttcgaaagat cgggctccgc cggtctatga cggccgatgg cgtgtggcag attttcgtgg
     4981 ccgttcgaaa ttacggcagg gcgccgcggc aggtctcgct tgggctgcgg ttcggcggtg
     5041 cccccgccgg ttcgcgcacg ctgacccttc caccggacag cgaacagaac atcacgtttg
     5101 aatataaaac gagagttgcc ggatggctgg aggcacggct gctcgacagg gacgatttcg
     5161 ccgccgacaa tcgcgccgtg ctggaactgc cagccatgcc gctgctgaaa gtcgcggtat
     5221 tttccgatga acccggcctt ctgcggccgg tactggcctc gaatcccggt gtggaggccc
     5281 gttttttgag gacctcgcag tacgatcccg gaaccgacgc gcaggtgata gttctggacc
     5341 ggttccggcc cgcccggctg cctgcggcgg ccgggaccct atggattgaa cccccatccg
     5401 cgggctcacc ggttcctgtc cgaaccgtcg tacagaatgc gccggtcgtt agctggaatt
     5461 cggaccacca actcggcgca ggccttcata ccaaagatct gcggctcggc tccgcacagg
     5521 tctttgccac ctccggaacg gacataccca ttgcctcggt ggaaaccgga cccgtcatcg
     5581 tcgcacgccc gggaacgccg aagcaggtag tggccggctt tcatcccatg cgttccggaa
     5641 tgcgatatga gctggctacg ccgctgctgt tcgcaaatat gctgcactgg atggcgccgg
     5701 aagtattccg gcgaaccgag atcatggccg gcagcgtggg atccatcgcc cacgagtttg
     5761 gctccgacgt gccggcaccc gatattagtt tgaccggcga aggccaggag ccgatcccgt
     5821 tcacgattca aggccggcag ttgcggtttt ttactttgat acccggtatc gtacggctca
     5881 agagccccga gggagaagcg gtctattccc taacgctgcc cgaggtgggt gagacccgat
     5941 gggagccgcc caaaaccgcg ctccggggga ttccgcggca gttcgccggt ttagcgcccg
     6001 caacggacat ctggccgtgg ctggcagtgt tgggcggaat aggactgctg gcggagtggc
     6061 tgttgttcgg ccgggcccgg cgttcgaatt tcttcgggcg cgctccggcg cgcatcacca
     6121 gcctggctgc acgcctgccc tggaaaaaca gaacgcgtgg acgtccggtg aggagggcat
     6181 catgactttt gcccatccct ggttgctgct gctggtcccg ctgcccgcgt tgtgggccgc
     6241 ctattcatgg cgcagcactt cgcgtaaagg ggcgctggcg ttaaaggcgc tctcgctggc
     6301 ggcgatcctg ctggcgctca cgcaaccgtc tgtcgatgtg gccggcacac gtatgtcggt
     6361 ttccgtgttg gtggacacct ctcaaagcat tggcgaggcc ggcatgagcc gcgcgtcctc
     6421 catagcgagt tctatccaga gccgccgcgg ccggcatcac gtggaaatcg tccctttcgc
     6481 ctctggcacc cgcgcgctgg cctccggtga aaagggtagc tccctgaaac tacggcggac
     6541 cgccggcgca ccgggaaacg ggacagacct ggaaaacgcg atccgcgagg cactggcttc
     6601 cgcgcccgcc ggcctgattc ctcgcctggt gctgatctcg gacggacgcg aaaaccaggg
     6661 tggtgtagtg cgcgccatgt ggcaagcgcg ggaactcgga gtgcccgtag atacgtatgc
     6721 agtcgagggg aagtcgaagc cgggtatcgt cctcgaaagt gtcagtgttc cttcgttcgc
     6781 attcacgggc gatcgggtgc cggtggagct cacactcgat tccccgcggg ccgccgcggc
     6841 cacggtagag gtgatggcgg gcgggaaggt tctgggaaag agcgaggttt ccctcggcgc
     6901 cggaaggaac caggtacgcg tctacgcaaa cctgggtgcc gtgggcgcta tcgatttttc
     6961 cggccgcgta tcgtctcccg atgtgggcga agttcagttt tcccaggcgc tgacgttgcg
     7021 caaaccgcgt gttctataca tttcgcagga tcctccggga acggaccttc acctgatgca
     7081 ggcgctgcga gcggcggagt tcgacgtcca gacggcggcc ggtccggcgc cccgcgatct
     7141 gtcggagtac cagttggtgg tattcaataa ctgggatttg gacgggatcg ctccgaaccg
     7201 gaaggaagac atcgagaagt tcgtgaaaga aggcggcggg gtgctggtca tcggtggaga
     7261 gcgcaacatc taccgggaaa aagaagggcc ggaagatccc ctggagcggt cgcttcccgc
     7321 caagttggcg ccgccgcggt cgcccgaagg cacttgtgtc gttctgatcg tggacaagtc
     7381 gtcatcgatg gaaggccgga aaatggaact ggcgcggctt gcctccatcg gcgtgatcga
     7441 aaacctgcgg ccgacggata tgatcggtgt gctgattttc gataattcct tccagtgggc
     7501 agtaccgatc cggcgggcgc aggaccgttc gctcatcaag cggctggtgg ccggaatcac
     7561 tccggatggc ggcacacaga tcgctcccgc gctgtccgag gcgtaccggc gtatccagcc
     7621 tgtccaggcc acgttcaaac atatcgtttt gttgacggac ggcatctcgg aagagggcga
     7681 cagtgtttct ctggccaagg aagccggttc ccaacgggtc acgatttcca cagtgggcct
     7741 cgggcaggat gtaaaccggg gatatctcga gaaagtcgca acattcgcca aaggcaaatc
     7801 gtacttcctg accgacccct ccggcctgga gcagattctg ctgaaggacg tcatggagca
     7861 cacgggcacg acaacggtcg aaaagctgat tcgtccggcg gtcgtaaaaa acgcggaaat
     7921 tctcgaccgc gtcggtatcg aaacggcgcc gccgttgagg ggctacgtcc ggttcacggc
     7981 aaaaccttcg gcggaaacca ttctgtccgt agagcgcgca gacccccttc tggtgcggtg
     8041 gcagtacggg ctgggacggg ctgctgtctt tacctccgat gccaaaagcc gctgggcgga
     8101 gcaatgggtg tcctggaacg ggtatgaccg tttctggtcg aatctgttcc gcgacctgct
     8161 gcctcatacc cagttgggcg aagcggtggt cgattacgac cgcgccacac gcgagctgat
     8221 cgtcgattac cggcttgcgc cgcacgttcc cgagccggag cagataccgg agatctatgc
     8281 gatggggccg gaaggcttcc agcgcccggt agaagtgaaa aaggtcgccg ccggaaccta
     8341 ccgcggccgt gtggccacgg gacaccggaa tgggctattc cgcctgcgcc cgctggcgga
     8401 atcgagagca tttccggaga tcggctatta ccgcgaagag caggaactgc gggactacgg
     8461 atccgattcc tttctgctgc aggaaatcgc gcgattcacg ggcggccgct tcaatcccga
     8521 tccgcgggat gtgttcgatg cgtccggccg ctccatcgaa acctctttgc gcctttggcc
     8581 cggcctgctg gctctggcgc tggcgctcaa tctggccgaa ttgattctcc gaaagtggcg
     8641 gggcattttc cagtggcggt gactggaatc acagactgtg atggtccgca ctcctagact
     8701 gaggatgtga aggatttcag cgaggcccct ttcctggtga tctgggaaac cactcaggca
     8761 tgcgacctgg cttgcgcgca ctgccgggcc agcgcggtcg agtgccgcca cccgctggaa
     8821 ctcaccacgg aagaggcgtt cgggctgctt cgcgaagtac gggaatttgg ggacccgctg
     8881 atggtcttca ccggcggcga cccgctgaag cgggacgata tcttcgatct gctccaatat
     8941 agtgtgcaac tcgggcttcg taccacgatc acgcccagtg caacgccgct actgacggct
     9001 tccgccatcg agcggtttaa gacctgtggt gtagcccgga tggccgtcag cctggatggc
     9061 cacgacaggg cgctgcacga tggatttcga ggtgtggagg gctcgttcga ccggacgatt
     9121 tcggctctcg atcatgcacg ggccgtgggt ctggataccc agataaacac caccgttacc
     9181 cggcggaatc tgcatgtgct ccacgagatc gccgcgcgcg ttgcggcttc cggcgcgaag
     9241 ctctggagtg tgtttttcct catcacgaca ggccgtgcca ccgccggaga cgaccttacg
     9301 gccgaagagt acgagcaggt attcgagttt ctgtataacc tgtccaaatc cgccccattc
     9361 gatatcaaaa cgaccgaggc ccagcactat cgccgttacg ttgcgcagag acgaaaggaa
     9421 gagggcggcg ccgtgcgaag cggccgcgaa gctccgccgt ccatcattca gcgccaggcg
     9481 ggcatcaacg acgggcgtgg tttcgttttc atctcgcata ccggcgatat ctatccaagc
     9541 ggatttctgc cggtgaaggg cggcaatgtc cgccaggagc gccttggcga catttaccgc
     9601 gactccgaac tgttccttac actgcgcgac agcgacctgt tggaaggcaa atgcggagac
     9661 tgcgaataca ggaacttgtg cggcggctcc cggtcgcgcg cttacgcact gaccggaaat
     9721 ttcatggcgt cggatccgcg ctgcgtgtac gagcccgccg tcaaaaggga acgcacccga
     9781 aa
//
LOCUS       DASTMK010000270         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_460121, whole genome shotgun sequence.
ACCESSION   DASTMK010000270 DASTMK010000000
VERSION     DASTMK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460121"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..2690)
                     /locus_tag="VFQ79_11780"
     CDS             complement(72..2690)
                     /locus_tag="VFQ79_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0140390.1"
                     /translation="MLWNAFFRRKRWERQLDSELRFHLEQQIRDYMATKGLSRREAER
                     MAYQDFGGIELSKEECRDQRPFLWLEHALQDTRYALRLLVRNRTFTVVAILSLALGIG
                     VASSVFSVVDRILFRSLPYHESDRLVSIGIGGPMLPYDFMFGATYLDFRRHQTEFSAV
                     TSWSGVSDCDLTSGEPVRVVCALVESTFLSTLGIVPVLGRSFTAEEDGPNKPKTVLLS
                     HGTWQSRFGGRPDVLGRTISIDGQLGRIVGVLPADFETPTLAHADLVIPQGLDEPTLQ
                     RAVTGRPLRVIARLRPGMTITEAQAAADLLVARALSGMPPGRLALEIKPHVKTLRDLQ
                     TGDAKGAAWVLFASVLVLLLLACANVANLILARTAGRRHELALRVALGAGKTRLIRQA
                     ITESSLLAFVGGLCGLALAHILLKILVNAAPDGIPRLTEATLDARVLAFTFVCALVSG
                     LAFGMGLPLAGSGPEALSSSRLTDGGRGRFRQALIVVQISFSLALLSAAGLLLQALWK
                     YQQIPIGIETQQVLTASLTLSPQRYPTAQRRLAFSEELEERLRSIGMFTTVAISDSHP
                     PNVPLRSKPLAVQKIDGRQQESPAQGTVVWRAVTPGYFRTLGIPIRKGREFTEQDRDR
                     GRDVMIVSQSLARRLFHGQEPVGRFIGSTQIVGMVSDVRNSGGTAPDDPEYYVPRSRA
                     ADAPIYSASDELQRVVAIVRTPLALALASQTVRNTIAQLDAALPVQIEPLHESVSRLS
                     VRPRFNAFVLAVFAAFGLVLAASGIYGVLGFLVAQRTREIGVRIALGATRHAIARMVL
                     GNALRWLAAGMIAGVALSIAVCRALKSLLYGLEVYDPLAWTAAAALLVFTAVFAAWQP
                     ARRAAGVDPVEALRHD"
     gene            complement(2700..3053)
                     /locus_tag="VFQ79_11785"
     CDS             complement(2700..3053)
                     /locus_tag="VFQ79_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0140391.1"
                     /translation="MSRHKDAGKEEAELLQGTLDMLILKVVALGPIHGYGIVQRIQRI
                     SKEALQIRQGSLYPALYRLESRGWLKAEWKMTEGGREAKFYSLTKTGERRLEDETRSW
                     NRLCAAISLVLEAAE"
     gene            complement(3159..>3787)
                     /locus_tag="VFQ79_11790"
     CDS             complement(3159..>3787)
                     /locus_tag="VFQ79_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140392.1"
                     /translation="NRFGVYPKKYSYFRYFWDYAGGHLTDSGIHVIDIVHMAFNERMP
                     RSVVAAGGKYWLQDDSETPDTMQVGFDYGSFLGAWEHRSNNTEGTTARLMGTTFHGAR
                     GTLYVDRALYRVTPEKGSGIEASEMKRTANPHPLHWANFIDCVRTRNKPNSDIETCVR
                     SSSACLLGNLSYQAKTRLDWDEQRQTVLQEDARPYLHREYRAPWKLEV"
BASE COUNT          803 a         1124 c         1129 g          731 t
ORIGIN      
        1 aagaacatcg gcatagagca gacctatttg ggcggaaaac tgatctacga acgacggtag
       61 accggctctc gtcaatcgtg acgcaatgcc tccaccggat cgacaccggc ggcacggcga
      121 gcgggctgcc aggcggcgaa gacagcggta aagacaagca aggcagcagc cgcggtccag
      181 gctaacggat cgtatacttc caaaccatat agaagggatt tcagcgcgcg gcaaacagca
      241 atcgaaagag ccacgcctgc aatcataccg gccgcaagcc aacgcagagc gttgccgagc
      301 accattcggg caatggcatg gcgtgtcgcg ccgagggcaa ttcgcacacc gatctcccgt
      361 gtccgctggg ccaccagaaa cccgagtacg ccgtatatgc ccgatgcagc caggactaga
      421 ccgaatgccg cgaacactgc gaggacgaac gcattgaatc ggggccggac agagagacga
      481 ctgacgcttt catgcaaggg ttctatctgt acaggcagcg cagcatcgag ctgtgcgatg
      541 gtattccgaa cggtctgtga agccaatgcc agcgcgagcg gtgtccggac gattgccact
      601 acgcgctgga gttcgtccga ggcgctgtat atcggggcat ccgccgcgcg gctgcgaggc
      661 acatagtact caggatcgtc aggcgctgtc ccgccactgt tacgcacgtc ggaaaccatc
      721 cccacaattt gagtactgcc gataaagcgc ccgacaggtt cctgaccgtg aaacagccgg
      781 cgcgcgagcg actggctgac gatcataacg tcgcggcccc gatcgcgatc ctgttctgta
      841 aactctcgtc ccttgcgtat cggaataccg agagtgcgga aatatccggg cgtgacagcg
      901 cgccacacca cggttccctg cgccggggat tcctgttgcc ggccatcgat cttctggacc
      961 gcaagaggct tcgaccgaag cggaacattg ggcggatggg aatcgctgat cgcaaccgtt
     1021 gtgaacatac cgatggagcg cagacgttct tccaactcct ccgagaatgc cagtcgccgc
     1081 tgcgccgtcg gatatcgttg tggcgagagc gtgagtgagg cggtcaacac ctgttgtgtc
     1141 tcgattccga tgggtatttg ctggtacttc cacaatgcct gtaacagtag tccggcagcg
     1201 ctcaatagtg cgagggagaa cgagatctgt accacgatga gagcttgtct gaatcttccg
     1261 cgcccaccat cggtcaagcg gctggaggag agagcttcgg gaccggaacc ggctaagggt
     1321 agacccatgc cgaatgcgag gccgctcacc agcgcgcaca cgaaggtgaa tgcgaggaca
     1381 cgcgcatcga gggttgcctc cgtcaggcgg gggatgccgt ccggagcggc gttgacgagg
     1441 attttgagca agatgtgcgc taacgccaga ccgcaaaggc cgccaacaaa cgccaggagc
     1501 gagctttccg ttatggcctg gcggatcagc cgcgtttttc ccgcgcccaa ggcaacgcgc
     1561 agcgccagtt catgccggcg tcctgctgtg cgcgccagaa tcaagttggc cacgttggcg
     1621 caggccagga gcagcagcac gaggacggaa gcgaatagaa cccatgccgc gcctttcgca
     1681 tcgccggtct gaagatcgcg cagggtcttg acgtgcggtt tgatttcaag cgcgagcctg
     1741 cccggcggca ttccggacaa cgcgcgggcg accaggagat cggccgctgc ttgcgcctcg
     1801 gtgattgtca ttcccggccg taagcgggca atgacgcgga gtggtctgcc cgtgacggcg
     1861 cgctgcaaag ttggttcgtc cagtccctgc gggatcacca ggtcggcgtg agcaagcgtg
     1921 ggtgtctcga aatcggccgg taatacaccc acgatccggc caagttgccc atcgatcgaa
     1981 atcgtgcgcc ccagaacatc cggacggcct ccgaatcgcg attgccaagt gccgtgggag
     2041 agcagaacgg tctttggttt gttcggccca tcctcttcgg cagtgaagct tctgccaagc
     2101 accggaacga ttccgagggt cgaaaggaaa gtggattcca ccagcgcgca caccactcgg
     2161 acgggttcgc ccgatgtgag atcgcagtcg ctcacaccgc tccaggaggt caccgctgaa
     2221 aattccgtct ggtgcctgcg gaagtcgaga taagtggcgc cgaacataaa atcgtaggga
     2281 agcatcggcc cgccaattcc aatggaaacc aaccggtccg attcgtgata gggcaggctg
     2341 cgaaagagaa ttcgatcgac aacgctgaaa acagaactgg cgaccccgat gccaagcgcc
     2401 agagagagaa ttgccaccac agtaaatgtc cgattgcgga ccagcagccg cagagcgtat
     2461 cgggtgtcct gaagggcgtg ttccagccag agaaatggcc gttgatccct gcattcctcc
     2521 tttgagagct cgattccgcc gaagtcttga tatgccatcc gttctgcctc tcgacggctc
     2581 aagccttttg tcgccatata gtcccggatc tgctgttcaa gatgaaatcg cagttcgctg
     2641 tcgagctgac gctcccagcg ctttctgcga aagaatgcgt tccatagcat tggatgcctc
     2701 tactccgcgg cctccaggac cagtgaaata gccgcgcaca aacgattcca actccgtgtc
     2761 tcgtcttcca accgccgctc gcccgttttt gtgagggagt agaacttcgc ttctcgtcca
     2821 ccctctgtca tcttccattc ggctttcagc catccacggc tttccaggcg gtagagggcc
     2881 gggtacaaag acccctggcg aatctgtagc gcctcctttg agatgcgctg gatccgctgc
     2941 acaatcccgt aaccatgaat cggacccaac gcgactactt tgagaatcag catgtcgagc
     3001 gtgccttgca gaagctcggc ttcttctttg ccagcgtctt tatgcctaga catcctagat
     3061 ataggtagaa tggatctcgc gggcggagat gtcaagcaga aagttgatac gaagccgcgc
     3121 gcgtgagcga gcggcttttg cacttgccgg ttattcggtc agacttcgag cttccacggc
     3181 gcccggtatt cgcggtgcaa gtacgggcgg gcatcttcct gcagtacggt ctgcctctgt
     3241 tcgtcccaat cgaggcgcgt tttcgcctgg taggatagat tgccgagcag acaggccgag
     3301 gaggagcgca cgcaagtctc gatatcgctg ttaggtttgt tgcgcgtccg gacgcaatcg
     3361 atgaagttgg cccagtgcag cggatgcggg ttggcggtcc gtttcatctc gctggcttcg
     3421 atcccggagc ccttctccgg agtgacccga tagagcgcgc gatccacgta gagcgtgccc
     3481 cgtgcaccgt ggaacgtcgt tcccatcagg cgcgccgtcg ttccttcggt gttattgctg
     3541 cggtgctccc aagcgcccag aaacgagccg tagtcgaagc cgacctgcat cgtatccggc
     3601 gtctcactgt cgtcctggag ccagtacttg ccgcccgcag ccaccaccga ccgcggcatg
     3661 cgttcgttaa atgccatgtg cacgatatcg atgacatgga ttcccgaatc ggtcaggtgc
     3721 cctccggcgt aatcccagaa atagcggaaa tacgaatatt ttttcggata gacaccgaag
     3781 cggttcg
//
LOCUS       DASTMK010000271        20247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_460172, whole genome shotgun sequence.
ACCESSION   DASTMK010000271 DASTMK010000000
VERSION     DASTMK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20247
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460172"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFQ79_11795"
     CDS             <1..920
                     /locus_tag="VFQ79_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140393.1"
                     /translation="GRHLRQRRNLNAIPYGTNFNPDSRDNSLPGNVPLPSTFLRPYRG
                     FGDISYIEFSSSSNYHSLQTQVNRRFTRGLTLGVSWTWSKSMDLADSDTILNPFLDQR
                     MRHYGKAGFDRTHNFVFNYNYFLPKFSQMWNNPLTRWVLDNWAVSGITTFMSGAPIGI
                     GYSLVQAVDLTGAAGAGVDSRVVLTGNPNLPKSERNISRHFRTEVVKPPAREDFGIGN
                     APKDPIRGPGINNWDISIIRDFPFTGDSVRNLQFRFEMYNAFNHTQFSGVDTGARFDA
                     AGNQVNARFGEYTAARPARRIQLGLKLNF"
     gene            946..2166
                     /locus_tag="VFQ79_11800"
     CDS             946..2166
                     /locus_tag="VFQ79_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140394.1"
                     /translation="MSLNFNRRDFFRTAGGAVSMAAAPALTAIQSGAKPVRFGLIGCG
                     GRGRHDATVLATQAGGQIVALADLLEDKIGPAKRHFDELLKKQQMPPIEATRLYHGWQ
                     SARRLAESDLDAVIIAITPYYYPMVLEMVAGTGKHIYCEKPVATDVAGSLKVIDIAKR
                     LDGKVVFHVGLQVPSATAMQEMARRIHSGAIGDIVSAQAFFLWGGGGRKAPPTKTPEE
                     ARIRTWTGDRILSGDIIVEQNVHSIDKINWILKAHPISAFAKGARRARTDYGDIWDHF
                     VGQFTYPNGVLVSFHSTQFLKGWSDAGERFFGTRGSSESHYTGGVRIYGEEAWDSGVN
                     QIVPDAETNKMRQFMNDIVTRNYRNEGFRGAESTLSAILLRTAAYEGREVTWDSVFAS
                     NQSWDPHIDLKQFG"
     gene            2173..2991
                     /locus_tag="VFQ79_11805"
     CDS             2173..2991
                     /locus_tag="VFQ79_11805"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0140395.1"
                     /translation="MMKLTDNVAIVTGAGSARGIGRAIALAFAREGAHVALTQWPGES
                     TASEIEAMGRRTLAAAIDVADASAVRDFVNRVADTFGRLDILVNNAGFCEFTPFLDIS
                     DELWDRTMAVNLHGYFSFGQEAARRMIGFGSGGKILNISSQAATVAGEEKVHYCVSKA
                     AVQMLTKGMALELAPHGINVNAIAPGTIDTDIIKQENIAALVESERSHSTIPLGRMGT
                     AADLADAAVFLCSPSAAYITGATLLVDGGMLTGSRLPPEFRSSAFRIKKSGATD"
     gene            complement(2988..3773)
                     /locus_tag="VFQ79_11810"
     CDS             complement(2988..3773)
                     /locus_tag="VFQ79_11810"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU0140396.1"
                     /translation="MSRGLGLFLTVGSFVIAAAQTIPGHDSTRKQVLLLTGYNSTRNH
                     DWRKLSPLLRQILEDTKDFEVRINEEPRALTQEVLRGYDVLLLDYSNYTPALAPPWPS
                     ETQAAYLEFLRNGGGVVAYHAACGSFQDWPEYRKSLGIAAHDRISHGPYQTFQVRTTA
                     SGKDLLQTLPAQWSAWGEIYNGMRLEEEARVLATAFDNPKNCSGGGKVCGSGKHEPVI
                     WTHYYGRGRSVVFTLGHDEKTFAAREIAPAFVSCVRWAASPTP"
     gene            complement(3781..4071)
                     /locus_tag="VFQ79_11815"
     CDS             complement(3781..4071)
                     /locus_tag="VFQ79_11815"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEU0140397.1"
                     /translation="MRLAYTMKLRPGKRDAYIDSHRNVWPELIAAAQASGIRNHSVFL
                     QGDTIFLYLEADDPKRALEELGAQKVKQDWDRFMREFLFPDISACEEAFHME"
     gene            complement(4068..5300)
                     /locus_tag="VFQ79_11820"
     CDS             complement(4068..5300)
                     /locus_tag="VFQ79_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0140398.1"
                     /translation="MKPHRWAVVALLVAGVVINYVDRGNLSVAAVDVMQDLDIKPGAM
                     GVLLSSLFWTYALFQLPCGYFLDRFGLKRTYAIAFLLWSLSSAAIGFAQTFEQVLILR
                     LILGVGEAVVTPASIAFIRTNFAEHEQGLPTAVFVGGMMMGPAIGTLFGGLLLETVGW
                     RSLFIWTGLAACVWVVPWMLIVREPPARDSIETKAPVPAVLASLLHWRLFWCVSIGAV
                     FYSYFWYFCLNWLPSYFLLAWKLSPSQMAVATAVPLAFMVVLSLSSGRVADRLIRRGR
                     NPLRVRAVFAAAGFCGGACLFLLPAATTTWQAFAVLLVSLGSVGVASANYWALTQSAV
                     PAVLIGRAIAYQNTLGNVGGISAPLISGILIGTSSNFFASIWLAAAAILVAGLSYATL
                     LSSPSRLRPLTIETHNRI"
     gene            5374..6300
                     /locus_tag="VFQ79_11825"
     CDS             5374..6300
                     /locus_tag="VFQ79_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0140399.1"
                     /translation="MIQGGWTRRGLLGAGLAGSTGLAHPSGTPIRLGGPIFLKSDDPE
                     ALAREHRRLGYSAAYCPKVDRSDVARLTAIDRAFRAQEVVIAEVGAWVNMLDVDPEKR
                     RKNLSYVAERLEIAEAVGAACCVNIAGSFSTKLWYGPDPRNVTKEYFDATVENCRKLI
                     DQVRPKRTTFSVEMSPWNLPDGPDEYVRLIKAVDRPAFAVHLDICNIVNSPRRLYSNA
                     VLIKECFEKLGPKLVSCHAKDLDWEPGMQIHFREVVPGRGQMDYRVWLRCMASLGRSV
                     PLMLEHLKSAQEYDDGRAYIEGIGREIGVSFA"
     gene            6297..7082
                     /locus_tag="VFQ79_11830"
     CDS             6297..7082
                     /locus_tag="VFQ79_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0140400.1"
                     /translation="MTGAQPLAGRTALVTGAARGIGRAIALRLARDGAAVAVNYLRSA
                     ERAEEVAGRISHNGGKAAAFRADVAAAEEVQRLASDVRVKLGPIDVLVCNAGQLIPGD
                     LEAYDSTAFAAMWNLHVGAVAHLANAVREDMSKRRYGRIVVVGSIAGIGTTFPGNTFY
                     GATKAAAMLLTRRLALELGPLGITVNAVAPGFIDTEMAHSATGRGRFDSAVAWVTEHS
                     MLRRIGYPEDVAHAVAFLASPEAGFITAQVIAADGGRMDYLSH"
     gene            7104..8330
                     /locus_tag="VFQ79_11835"
     CDS             7104..8330
                     /locus_tag="VFQ79_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140401.1"
                     /translation="MIHNHGVPRRNFLQAAAAGIAATSYAGGQTPARHSGPVRLGLIG
                     CGGRGRFVASEIVNKADGQIVALADLFEDRVTAAKTQFDQLNRQRGVAPIDSSRIFTG
                     VDAAANVVASDVDAVLIAITPYFYPQVLEFAVAAGKHIYCEKPVATDVAGCMKVAQLA
                     ARADGKLTFHVGFQLPYVPALQELVRRVHDGAIGEIITGEGCFYYGGATRTPPSGESG
                     QRARMRVWHGDRVLSGDIVVEQNVHSIDKINWMMRGHPIAAVATGGRSARKDYGDVWD
                     HYNALLRYPNDVTVSFRSTQFLRGWNDVAERFFGTKGVTEAHYDGPVAIHGDHVWDAG
                     VKGTRVGAEAYKVRAFINDIVTGQYRNEGFRGAESTLSAILIRTAAYEQREVTWDGLI
                     SSNQRWEPPVDLKQIV"
     gene            complement(8526..8828)
                     /locus_tag="VFQ79_11840"
     CDS             complement(8526..8828)
                     /locus_tag="VFQ79_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140402.1"
                     /translation="MSVVYGRFAPLAPRVIDPEIVHDADEPGLRLLQPIALLQEPEQA
                     HETARDQIICVGRPAAAQDEVLVQGIIMLRVQLLDDPEGTPLTAGTVTLPMKRPQL"
     gene            8936..9454
                     /locus_tag="VFQ79_11845"
     CDS             8936..9454
                     /locus_tag="VFQ79_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140403.1"
                     /translation="MADESWEVKNGHPPAGVLLLYLEAELEGRLAAAVERHTRACWTC
                     RAQCERMERGIRSFVDFHETAEIPPPPAGRRTFLSRLREQAPVATSGAGGGWNAGNSV
                     VYGVPLTPHLDTTSLAVAALRFHYHLPEVRQSLSWLLAAKCSSAYSLTWKILALRSYL
                     DVRSDVGPRPVS"
     gene            9487..11571
                     /locus_tag="VFQ79_11850"
     CDS             9487..11571
                     /locus_tag="VFQ79_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140404.1"
                     /translation="MYMARLDWNHSSRHMLSGNLYVDDNGQSTEMVGGNIPGYVGNRQ
                     DQKTTMVTLNDTYTFSPNVLNQAMVSFLRTYSLNDATQTIGPAALGIGMPQYSESGAP
                     AVDVTGYFNFFGSYGRVLFTNHNWHFRDDVSWMHGRHSFKFGGEVLKLWWRQVFMGNP
                     TFTFNGSRTGDAFADFLVGAYRNFNTPFGVRENDNLTTLPAFYFQDEYRLNSRLTLNF
                     GLRYEPFLPWVDRYDRLSAFAPDQKSTVFPDAPPNLLFARDPGLGRGIIGRDLNNFAP
                     RFGFAWDVTGNGTTSLRGGYGIYYDLIKADSVAQENAPWAGNVQNFDGRFDSPFGALG
                     QVTPPVTPNQFGCSARKSFPGVDCDLFPLPLAGFYLPLDMSTAYVQSWNLTLQRQITS
                     TVMIQSSYIGKIGTKIEARRNVNPARFINDPITGGAPSLQNVNNRVIFAPGIISPLAK
                     MMSNDHRNWYHSWQTQLTRRFARGLSLSAAYTLSKSLDTLSNNIYSRLFDSPFDLSYN
                     RGRSDFDRRHAFVASWVWSPEMRFESGMLNAILANWTLTGIHTLQSGLPMTFAMGDDV
                     ALDGTGGRQHAQLTGDPIAREHPSRGDMIAQFFNTGAFVPTALVPRGIYGNSGRNILS
                     GPGMANNDMSVMKDIPVSERNRLQFRAEFFNAFNQVRLSNPDSTVNSQNFGRIRSAGS
                     PRVIQFALKFLW"
     gene            12157..13005
                     /locus_tag="VFQ79_11855"
     CDS             12157..13005
                     /locus_tag="VFQ79_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0140405.1"
                     /translation="MIRQLRLTVLADNSVAAPNLLAEHGLSILIEADGRRILFDTGQG
                     KVLSANADSLGAGLSALDAVVLSHGHFDHSGGLASLPEGDGPAAVFLHPAALQSKYAT
                     SDTPPHRSIGMPEHSRQALDRFWKRIVWTQGATEVVPGVWCTGEIPRLAPPDPRSTPV
                     FFCDADCTKPDPIPDDQALFIEMQDGLVVIAGCAHSGVINTLDRIGTLARQADLLALI
                     GGFHLGRASYKQLEECGNAIGRRNCQFLAPCHCTGMSAHAYLRSRFHSLVQDVGVGTR
                     LIIGHQ"
     gene            13002..13481
                     /gene="tsaA"
                     /locus_tag="VFQ79_11860"
     CDS             13002..13481
                     /gene="tsaA"
                     /locus_tag="VFQ79_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEU0140406.1"
                     /translation="MKRIRIQIVGVIETPFREPAGCPIQPSRAGGAEGTVRVDPRFAE
                     GLKDIEGFERIWLTYWLHRASCSTLLVTPFLDKQQRGIFATRAPSRPSPVGLSVVRLL
                     GVEGNILHVADVDMIDGTPLLDIKPYVPEFDSYPHSRAGWFDDSGSQRCLADDRFDR"
     gene            13689..14144
                     /locus_tag="VFQ79_11865"
     CDS             13689..14144
                     /locus_tag="VFQ79_11865"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0140407.1"
                     /translation="MRAKKGAEEYVPRRVCLRHGKGHPCTCAMGNLYRFVEPVALLLL
                     KKKGRSYGYDLAGDLREYTLTDAEIERAALYRTLRQLETNGNVSSEWDTDGGGPARRV
                     YRLTPGGERHLEEWTSVLDHVSKSMAHFVKDARESLSNPIKPEPNHVDC"
     gene            14131..14898
                     /locus_tag="VFQ79_11870"
     CDS             14131..14898
                     /locus_tag="VFQ79_11870"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR00292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HEU0140408.1"
                     /translation="MLTAEWNESLISKAIIESYHQKLLRSVESNIVIVGAGPAGLTAA
                     SLLAAQGRKVTVLEKRLAPGGGVWGGGMGMNQVVVQEEAVPLLDAAGVRHSRCGNLYV
                     VDAVELACTLCLQALHAGAVLLNLTTLEDLCVQRDKVTGVVVNRTTISGALPVDPIVL
                     RADVVIDGSGHEAVAVDRLRARNLLPPHLTSRSCEGPMDAAAGEAFVVEHAGEVYSGL
                     WVTGMAVCATFGGPRMGPIFGGMLLSGKRVAERILAQ"
     gene            14895..15593
                     /locus_tag="VFQ79_11875"
     CDS             14895..15593
                     /locus_tag="VFQ79_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU0140409.1"
                     /translation="MKRIGILGGMSPESTALYYEHITRTYTAQFGDYGYPEILIYSVS
                     FQHYVEWQRNGQWKEAAEEMASALERLRLAGADFGLIATNTMHYVFDQVQQAVGMPLL
                     SIVDATVEAILAAGLQTIGLLGTTFTMRERFFLDGLERAGICALVPDVCDQVRVHDVI
                     FQELCRGEVRPESQQFYMDIVDQLRGRGAEGIILGCTEIPLLLKPEQCDVPLFNTALL
                     HADKALRLALQPEH"
     gene            15595..16050
                     /locus_tag="VFQ79_11880"
     CDS             15595..16050
                     /locus_tag="VFQ79_11880"
                     /inference="COORDINATES: protein motif:HMM:NF020400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HEU0140410.1"
                     /translation="MKDRTVRIGIIICARYRDYGGGKCFRALRQRQGAFSRYAPEECV
                     EIVGYSTCGGCPGGNVEYVPAEFVKNDANVVHLATGLVVGYPPCPHIHQFKAFIEKRY
                     GLTVVVGTHPIPLKYLQTHNQLPFWEESGMADIASHLLAEPRDVMEAYN"
     gene            complement(16047..16553)
                     /locus_tag="VFQ79_11885"
     CDS             complement(16047..16553)
                     /locus_tag="VFQ79_11885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0140411.1"
                     /translation="MTRDQVLAALNLKPGQVVADIGAGTGYFMSRLARDASAPKVYAV
                     DIEPAMVEHLKQRAEKEGLTNVTAIRASSTSPNLPEPVDCVLIVNTYHHIPQREAYFR
                     ILGKSLKPGGRLAIIDFKKGAPGGPPDHYRFTADEIKAELERAGFKLLVQHNFLPRQE
                     FLILHQTR"
     gene            16976..17326
                     /locus_tag="VFQ79_11890"
     CDS             16976..17326
                     /locus_tag="VFQ79_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140412.1"
                     /translation="MQATGSRIGRDSIGVERMQPLPVVFSGPVLRNRIFFAAAVERLH
                     LNQSAAWRMAVPSIEERRDAPVARRPVLDAFPLPSSPLEAEHTAQTRWPGDVLTNSVR
                     VDHAFSSNGLAFLR"
     gene            17366..19186
                     /locus_tag="VFQ79_11895"
     CDS             17366..19186
                     /locus_tag="VFQ79_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140413.1"
                     /translation="MNEAQLDSTGVTAGLLMSSGPRLTNDARINLSRATADSRWSASG
                     LGGAQPLDPASVLPPLSGTGRGVYALSIAGLGPLLAAEPARNRQTELNLIDTIAVHSG
                     NHQAKFGIDYHRLVSTRAEPISGVIASYTSLPDFIGGAEPAVDFLQAASGSSVIETFS
                     LFAQDTWQLGNRLNFTFGTRWEITPAPTYRSPNGTSAIRLISGEIPPRERPDPGEPTP
                     FPASTAAVPSWKTRYGQFAPRAGVALRLDEEGSLIFRAGAGLFYDLGFSSVADVLNGV
                     PFNRWIVNLTAGAPGSGQREVLYGFAEDLKLPYSVHWNIAIEKLLNRRSAVSAAYVGS
                     PARRLLRLERRPAAVADQPGSVLASNGGSSQYHSLQLQARRSVAPGLNGFAAYTWGHS
                     IDNASWNSAIFFLYPGATDRGSSDFDVRHSFQTGLSYNLNSSAMGRWTRGWTVSTIFR
                     TRTGFLIDVVARENPFGLGSDNARPDLVDGVPVWIRDPSVPAGRRLNRDAFRIPAPGL
                     QGSLGRNAVRGFGLAQIDASLERRFTIGEAAALGIRLEAYNVTNRPNFADPMRLLNNP
                     LFGQSLSQANLMLGTGRAHSGLTPNFQSGGPRSLQLRIDV"
     gene            complement(19201..>20247)
                     /locus_tag="VFQ79_11900"
     CDS             complement(19201..>20247)
                     /locus_tag="VFQ79_11900"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEU0140414.1"
                     /translation="QAPQKIVVSPGGNLLVSETSTQPNAGRVSLVTPGGARRTLIEGL
                     PSGLDVAGGPSGPTAMAVSNRTLYLAIGGGDAERRGEAPGTAIHNPEGRSSALLASIL
                     RLRFGSDIDQITGSFRMTPEHQQSLMDGGEVQITDGSGASVSVDVLARLPISEPDPVT
                     IYRFSNPWGLASSGDGSVLWAVDASMNALMRIDPETGRWQRIVRFPPMQNPGAVGPPM
                     IDPVPTNVRTYGNELLVSFLTGFPFIPGTARVLIVDPDQRSSQPFMFGRTSVVDVLWR
                     DMGTSRAQFFALEFSSNQSAQPAAPGRLLRFDTPEAEVVADDLQGPVSMALDGQSNTL
                     YVLELSGRILRLGL"
BASE COUNT         4117 a         5979 c         6133 g         4018 t
ORIGIN      
        1 tcgggcggca tttgcgacag cgcagaaacc tgaacgcgat tccttacgga acgaacttca
       61 atccggatag ccgagacaat tcgctgcccg gaaatgtacc gcttccttcc acgttcctcc
      121 gcccctatcg agggtttgga gatatctcgt atatcgagtt ttcgagttcc tcgaactacc
      181 actcccttca gacacaggtc aaccggcgat ttacacgggg cctgacattg ggagtaagct
      241 ggacgtggtc taaatccatg gaccttgccg atagtgatac gattctgaat ccatttctgg
      301 atcaacgaat gcgccactac ggcaaggcag gcttcgacag gactcacaac tttgttttca
      361 actacaacta ctttctgccg aaattcagcc agatgtggaa caacccgctc acccgctggg
      421 tgctggacaa ctgggccgta agcggaatca ccacattcat gagcggagcg ccaattggca
      481 tcggatacag cctggtgcag gctgtagacc tcaccggagc cgcgggagcg ggagtggact
      541 cgcgcgtggt gctgaccggc aacccaaatc tgccaaagag cgagcgcaat atctcacgcc
      601 atttccgaac agaggttgtg aagccgccgg cgcgcgaaga ctttggaatt ggcaacgcac
      661 caaaagatcc catacgcggt ccaggaatca acaactggga tatctcgatc attcgagatt
      721 ttccgttcac tggcgactcc gtgcgcaacc ttcagtttcg gtttgagatg tacaacgcgt
      781 tcaaccacac acagttcagc ggcgtggaca cgggtgcacg gttcgatgcg gccggcaacc
      841 aggtgaacgc ccgattcggc gaatacacgg ctgcgcggcc ggcccgccga atccaactgg
      901 gtctgaagct gaacttctga acgaagaacg aggggagcaa ccggaatgtc attgaacttc
      961 aatcgtagag atttttttcg tactgccggc ggtgctgtca gtatggcggc cgctccggcg
     1021 ctcacggcga tacagagcgg cgccaagccg gttcgcttcg gcctgatcgg atgtggcggc
     1081 cgtggcagac atgatgctac ggtattggcg acgcaggcgg gcggccagat tgtcgcattg
     1141 gccgacctgc ttgaagacaa gatcggtccg gcaaaacgcc acttcgacga gttgctcaag
     1201 aagcagcaga tgcctccgat agaggctacg cgcctgtatc acggttggca atcggcgcga
     1261 cgcctggcgg agagtgacct ggatgcggtc atcatcgcaa tcactcccta ttactacccg
     1321 atggtcctgg agatggtcgc cggcaccggc aagcatatct attgcgagaa accggtcgct
     1381 acagacgttg caggaagcct gaaggtgatc gacatagcga aacgcctgga tggcaaggtc
     1441 gtatttcacg ttggcctcca ggtgccatcg gccacggcca tgcaggagat ggcccggcga
     1501 atccactccg gtgcgatcgg agatattgtc agcgcccagg cgttctttct ctggggcggc
     1561 ggcggccgaa aggcgcctcc gacgaagacg ccggaggaag cgcgaattcg cacctggaca
     1621 ggcgaccgga tcctttcggg tgacatcatt gtggagcaga acgtccacag catcgacaaa
     1681 atcaattgga tcctgaaggc tcacccaatt tccgccttcg cgaagggtgc acggcgcgca
     1741 agaacggact atggggacat ctgggatcac tttgtcggac agttcaccta tccaaacggc
     1801 gtacttgtca gtttccactc gacgcaattt ctgaaagggt ggtcggatgc gggagagcgc
     1861 ttcttcggta cgcgaggcag ttccgaatcg cattacaccg gcggcgtcag gatttatggc
     1921 gaggaggcct gggattcggg cgtgaaccag atcgtaccgg acgccgagac aaataagatg
     1981 cgccagttca tgaacgatat tgtgactcgc aactatagaa atgaaggctt tcgaggcgcc
     2041 gagagcacgt tgagcgcaat cctgcttcga accgcggcgt acgaaggacg ggaagtcacc
     2101 tgggactcgg tttttgcttc caatcaatct tgggatccgc acatcgatct gaaacaattc
     2161 ggatagtggt agatgatgaa gctgacagac aacgtcgcga ttgtcacggg ggccggatct
     2221 gcgcgcggta tcgggcgcgc catcgcgctt gccttcgccc gggaaggagc gcatgtcgcg
     2281 ttgacgcagt ggcccggtga gtctaccgca tcggagattg aagccatggg gcggcgtacg
     2341 ctcgcggcgg ctatcgatgt ggcagatgcc tccgccgttc gggattttgt aaaccgggtg
     2401 gcggacacat tcggcaggct ggacattctt gtcaataacg cagggttctg cgagttcacg
     2461 ccatttctcg acatatcgga cgaactctgg gatcgaacca tggccgtgaa tcttcatggc
     2521 tatttctcct tcggacagga ggccgcaaga agaatgatcg gcttcgggtc cggcggcaaa
     2581 atcctgaaca tttcgtcgca ggccgcgacc gtcgcgggtg aagaaaaagt ccactattgc
     2641 gtgtcaaaag ctgcggtgca gatgctgacc aagggtatgg cgctggaact tgcgccacat
     2701 ggcatcaatg tcaacgccat cgctcctggc accatcgaca cagacatcat caagcaggaa
     2761 aacatcgccg ctcttgtcga gagtgagagg agccacagca cgatcccgct tggacggatg
     2821 ggcactgccg cggatctggc ggatgcggcg gttttcctgt gttctccgtc ggcggcatat
     2881 atcacaggcg cgacgctgct ggttgacgga gggatgctca cagggtcgag actgccaccc
     2941 gagtttcggt cttccgcgtt tcgcatcaag aaatctggtg cgacggatta gggtgttggc
     3001 gaagcagccc accgtacgca ggagacgaac gccggcgcga tttcgcgcgc cgcgaatgtc
     3061 ttttcgtcgt gcccaagtgt gaaaacgacc gaccggccac ggccgtaata gtgtgtccag
     3121 atgaccggtt cgtgtttgcc tgaaccgcag actttaccgc cgccggagca gttcttcggg
     3181 ttatcaaagg ctgtggcgag tacgcgtgct tcttcctcca gccgcatccc gttgtaaatt
     3241 tcaccccagg cagaccactg cgccggaagt gtttgcagaa ggtcctttcc gctcgcagtc
     3301 gttcggacct gaaacgtttg atacggcccg tgactaatcc tgtcatgagc ggcgatgccg
     3361 agagacttcc ggtactcggg ccagtcctgg aaggatccac acgcggcatg atacgcgaca
     3421 actccgccgc cgttacgcag gaattccaga taagccgcct gcgtttcgga gggccacggc
     3481 ggagcgagcg cgggtgtgta attgctatag tccaacaaca acacgtcata gcccctgaga
     3541 acttcttgtg ttaaagctcg cggctcctcg ttgatccgga cttcgaaatc tttcgtatct
     3601 tccaggattt gccgcagcaa cggagacagc tttcgccagt catgattgcg tgtggagtta
     3661 taaccggtga gtagaagcac ttgcttgcga gtcgaatcat ggccagggat cgtctgggcg
     3721 gccgcgatca cgaagcttcc aacagtaagg aataaaccaa ggcctctaga catacttctg
     3781 tcactccatg tggaacgctt cttcgcacgc cgagatatct ggaaacagaa actccctcat
     3841 gaaccggtcc caatcctgct tgaccttttg tgcgccaagc tcttccagtg cgcgtttagg
     3901 gtcgtcggcc tccaggtaca ggaaaatcgt gtcgccttgc aggaagacgg aatggttgcg
     3961 gataccggat gcttgagccg ccgcgatcag ttcgggccag acgtttcgat gtgaatcgat
     4021 gtaagcatcg cgcttgccgg gacgcagttt catggtgtaa gcgagtctca tattctgttg
     4081 tgcgtttcta ttgtcagggg tcgcagtctg gacgggctgc ttagaagggt cgcatacgac
     4141 aagccggcta ccaggatggc agcggcggca agccagatcg atgcgaagaa gttactggag
     4201 gtacctatca gaatcccgct gatcagcggc gcggaaatgc ctcccacatt gcccagagtg
     4261 ttctggtacg cgattgcgcg cccgatcagc acggcgggca ccgcggactg tgtcaacgcc
     4321 cagtagttcg cggaagcaac gccgaccgaa ccgagcgaaa ccaaaagaac ggcaaacgcc
     4381 tgccaggtgg ttgtggccgc cggcaacagg aacagacagg caccgccgca gaacccagcc
     4441 gccgcaaata ccgcccggac acgaagtgga tttcgtccgc gacgaatcag ccggtcggcg
     4501 accctacctg aggagaggga gaggacaacc atgaacgcca gcggcacagc ggtcgcgaca
     4561 gccatttgcg agggcgagag tttccaggcg agcaggaagt acgagggcag ccagttcagg
     4621 cagaaatacc agaagtacga atagaacacc gcaccaatgg agacacacca gaaaagacgc
     4681 caatgcagaa gactggctaa aaccgccgga acaggcgcct tcgtctcaat ggaatcgcgc
     4741 gccggcggtt cccttacgat tagcatccag ggcacgaccc agacgcacgc cgcaagtccc
     4801 gtccagatga acaacgatct ccatccaacc gtctccagca ggaggcctcc gaagagcgtg
     4861 ccaatcgccg gacccatcat cattccacca acaaacacgg ccgtgggcag cccttgctca
     4921 tgttccgcaa agttcgtacg gataaacgcg atgctcgccg gtgtcaccac ggcttcacca
     4981 actccaagta tcagccgtaa aatcagcacc tgctcaaaag tctgcgcaaa gccgattgca
     5041 gccgacgata gcgaccagag gagaaacgca atggcgtacg ttctcttcag cccgaacctg
     5101 tccaggaagt agccgcacgg caattggaaa agtgcatacg tccagaagag cgaagagaga
     5161 agcacgccca tggccccggg cttgatgtcg agatcctgca tgacatccac ggcggcgaca
     5221 ctcaggtttc cacggtcgac atagttgatt actacgcccg caacgaggag cgccaccacg
     5281 gcccatcgat gtggtttcat ggaggtccgg cgccttttca aattcgttga cggcggcaac
     5341 gctgtattgt actattgatt ttctatcaat acaatgatac aaggcggatg gacgcgcaga
     5401 gggctgcttg gcgcagggct ggcgggatcg acggggctgg cgcatccttc cggtactcca
     5461 atacgactcg gaggaccgat ttttctgaag tccgatgacc cggaggcgct tgcgcgggag
     5521 caccggcggc tgggatacag cgccgcatat tgcccgaagg tcgatcgaag cgacgtagcg
     5581 aggctcacgg ctatcgaccg tgcgtttcgc gcgcaggagg tggttatcgc cgaggtgggt
     5641 gcttgggtga atatgctcga cgttgatccg gagaagcgcc gtaaaaacct gagttacgta
     5701 gcggagcggc tggagatcgc ggaagcagtc ggggctgcat gctgtgtgaa catcgccgga
     5761 tctttcagca caaaactttg gtacggaccg gacccgcgca acgtaacaaa ggaatacttc
     5821 gatgccaccg tggagaactg ccgcaaattg atcgaccagg tccggccgaa acgtacaact
     5881 ttcagcgtcg agatgtcgcc atggaacctc ccggatggac cggatgaata cgtgcggctc
     5941 atcaaagcgg tggatcgtcc ggcatttgca gtgcacctcg acatctgtaa catcgtgaac
     6001 agtccccgca ggttgtattc gaatgcagtg ttaatcaaag agtgcttcga gaagctcgga
     6061 ccaaaacttg tgtcgtgtca tgcaaaggac ctcgactggg aaccagggat gcagattcat
     6121 ttccgcgagg ttgtgccggg tcgcggccag atggactaca gggtatggct gcgctgcatg
     6181 gcctcgcttg ggcgctcagt acccctgatg ctggaacatc tgaaaagcgc ccaggagtac
     6241 gacgatggca gggcctacat cgaaggtatt ggacgcgaaa tcggggtatc gttcgcttga
     6301 ccggagctca accattagca ggccggacgg cattagtaac cggggccgcg cgcggcattg
     6361 gacgtgcgat tgcgctacgc ctggcgcggg acggtgcagc agtggccgtc aactatctgc
     6421 gatctgcgga gagggcggag gaagtggccg gtcggatttc gcacaacgga gggaaggcgg
     6481 ccgctttcag agccgatgtt gcggcggcag aggaggtaca gcggctcgcc tccgatgtcc
     6541 gggtaaagct gggacctata gacgtcctcg tgtgcaacgc gggtcaactc atccccggag
     6601 atctcgaagc ctacgattcc accgcgtttg ccgccatgtg gaacttgcat gtgggagcgg
     6661 ttgcgcatct ggccaacgcc gttcgcgagg acatgagcaa acgccgttac gggcgcattg
     6721 tagtcgtggg atccatagca ggtattggta caacttttcc gggaaatact ttttatggtg
     6781 caacaaaagc agccgcgatg ctgctgaccc gcaggctggc tctggaactt gggcccctgg
     6841 gtatcacagt gaacgccgtt gcgcccgggt tcatcgacac agagatggcg cactccgcga
     6901 ccggacgcgg ccggtttgat agcgcagttg cctgggtaac ggaacacagc atgctcaggc
     6961 gcataggtta tccggaggat gttgcgcatg cggttgcatt cctggcttct ccggaggccg
     7021 gattcatcac ggcacaagtg attgcagccg atggcggccg gatggattac ctcagccatt
     7081 aagcacgcat caggagacac tcgatgattc ataaccacgg cgtaccccgc cggaatttct
     7141 tgcaggccgc agccgcggga atagccgcaa cttcctatgc cggcgggcag acgccggcgc
     7201 ggcatagcgg gcctgtccgg cttggtctga ttgggtgtgg cggtcgcgga cgcttcgtcg
     7261 ccagcgaaat cgttaacaag gcggatggcc agatcgtagc gcttgcggat ttgttcgagg
     7321 accgggtgac ggccgctaag acgcaatttg accagttgaa ccggcagcgc ggtgtagctc
     7381 caatagactc ctcccggatc ttcaccggtg tcgacgcggc cgcgaacgtc gtggcttcag
     7441 acgtggacgc cgttctgata gccataacac cgtatttcta tccccaggtg ctcgagttcg
     7501 ccgtcgcagc cggtaaacac atctactgcg aaaagcctgt ggctacagac gttgcggggt
     7561 gtatgaaagt ggcacaactg gcggcgcgcg cggacgggaa gttgaccttt catgtcggct
     7621 tccaattgcc ctatgtgccc gcgctgcagg aactcgtacg ccgggtccac gacggcgcga
     7681 taggcgagat catcacgggc gagggctgtt tttactacgg tggcgcaacc aggaccccgc
     7741 cttcggggga aagcggtcaa cgcgcccgca tgcgtgtctg gcatggagac cgcgtgctct
     7801 ccggcgacat cgtcgtagaa cagaacgtcc atagcatcga caagatcaac tggatgatgc
     7861 gcggccatcc tatcgcggcg gtggctaccg gcggacggag cgcacgtaaa gactacgggg
     7921 atgtctggga ccactacaac gcgctcctcc ggtaccctaa cgatgtaacg gtgagctttc
     7981 gctccacgca gttccttcgc ggttggaatg acgtggcgga acgtttcttt ggaacaaagg
     8041 gtgtgaccga ggcacactac gatggacctg tggcgattca tggcgatcac gtgtgggatg
     8101 caggcgtgaa agggacgcgc gtgggcgcgg aggcctacaa agtgcgcgcg ttcatcaatg
     8161 acatcgtcac cgggcagtac aggaacgagg gatttcgagg agccgagagt accttgagcg
     8221 cgattctgat tcgaacagcg gcgtacgaac agcgtgaagt gacctgggac ggtctgatca
     8281 gttcgaatca gcgatgggaa ccgccggtag acctcaagca aatcgtttaa tcccggctat
     8341 ctggtgtgga tcccaggaag aaactgccag cggcgagccc gaacgtggct gagcccatca
     8401 cctgaattcc ggcgtccggc ttcgtctcaa tagagggtgg tagtccgtcc gtggtctgtt
     8461 cgtgtacccc tacggagacg tgaaggagta tcgatttgat ctccaaaagc gtagttgtgg
     8521 ccttttcata gttgtggcct tttcattggg agagtgacag taccggcagt caacggagtc
     8581 ccttccggat cgtcgaggag ttgtacgcgg agcattatga tgccctgtac caatacctca
     8641 tcctgagcgg ctgccggcct gccgacgcag atgatctgat cccgagcggt ttcgtgcgcc
     8701 tgctccggtt cctgcagcag ggcaatgggc tggagaagcc gaagccctgg ctcatccgcg
     8761 tcgtgcacaa tctccggatc gatgacgcgc ggcgcgagcg gcgcgaatcg accatagacg
     8821 acggacaatt ggaggcacgc atgacggagt cttcggcacc atgccagacg tggagacgga
     8881 gttgctgcag ccgagatatg cgcccgggcg acggagcggt tgggacggat cattcatggc
     8941 tgacgaatcg tgggaagtaa agaacggaca cccgcctgcc ggcgtgctcc tgctctatct
     9001 ggaagcggag ttggagggcc ggctggccgc tgccgtggag cgtcacaccc gcgcttgctg
     9061 gacgtgccgt gcacaatgcg agcgcatgga gcggggcatt cgctcctttg tggacttcca
     9121 tgaaaccgct gagattcccc cgccacctgc cggaaggcga accttcctct cgcgcctgcg
     9181 cgagcaagcg ccggtcgcca cctccggcgc aggcgggggc tggaacgccg gcaacagcgt
     9241 cgtgtacggc gttccgctga caccccatct tgacaccaca tccctcgccg ttgcggcact
     9301 ccgattccac tatcacctgc ccgaagtcag gcagtccctg tcctggctcc tcgccgcgaa
     9361 gtgctcgtcg gcctacagcc tgacgtggaa gattctcgca ctgcgaagct acttggacgt
     9421 ccgctccgat gtgggcccgc gtccggtcag ttagttaccc tttcgccaaa cccgagaaac
     9481 gacgacatgt acatggccag actggactgg aaccacagct cgagacacat gctgtccggc
     9541 aacctgtacg tggacgacaa cggccagtcg acggagatgg tcggcggcaa catcccaggg
     9601 tacgtaggga accggcagga tcagaagacc acgatggtga cgctgaacga cacctatacg
     9661 ttcagcccca acgttctgaa ccaggccatg gtttcattct tgcgcacgta ttcgctgaac
     9721 gacgccaccc agaccatcgg gccggcggcg ctcggtatcg gtatgccgca gtattctgaa
     9781 tcgggcgcgc cggccgtgga cgtcacggga tacttcaact ttttcggatc gtacggccgc
     9841 gtcctgttta caaatcataa ctggcacttc cgcgacgatg tgagctggat gcacgggcgg
     9901 cacagcttca agttcggcgg cgaggtgctg aagctctggt ggcgccaggt ctttatgggc
     9961 aacccgactt tcacgttcaa cgggtcgcgc accggcgatg cgttcgcaga ctttctcgtg
    10021 ggcgcgtacc gcaacttcaa cacccctttc ggcgtccgcg agaatgataa cctcaccacg
    10081 ctgccggcgt tttacttcca ggacgaatat cgcctgaact cccggctgac gctcaacttc
    10141 ggcctgcgct acgagccctt cctgccctgg gtcgatcgct acgaccgcct cagcgcgttc
    10201 gctccggacc aaaaatccac cgtgttcccc gatgcgcctc cgaacctgct gttcgcgcgc
    10261 gatccgggat tgggccgggg catcatcggc cgcgatctga acaacttcgc gccccggttc
    10321 ggtttcgcct gggacgtcac cggaaacggg actacaagcc tccggggcgg ctacggcatc
    10381 tattacgacc tcatcaaggc ggattcagtt gcgcaggaaa acgcgccgtg ggcaggcaac
    10441 gtccagaact tcgacggccg gttcgatagc cccttcggag ctcttgggca ggtcacgccc
    10501 ccggtgacgc cgaaccagtt cggatgctca gcgcggaaat cctttcccgg cgtggattgc
    10561 gacctctttc cgctgccctt ggctgggttc tacctcccgc tcgacatgag cacggcctac
    10621 gtccagtcct ggaacctcac gctccagcgg cagatcacct cgacggttat gatccagagt
    10681 tcgtacatcg gcaaaatcgg gacgaagatc gaggcgcggc gaaacgtgaa ccccgcgcgg
    10741 ttcatcaacg acccgatcac cggcggggcg ccatcgctcc agaacgtgaa taaccgcgtc
    10801 attttcgcgc cgggcattat ctcgccgctg gcgaagatga tgtccaacga ccaccgcaac
    10861 tggtatcata gctggcagac gcagttgacg cgccggttcg cccggggatt gtccctctct
    10921 gccgcgtaca ccctgtcgaa atcgctcgat acgttgtcga acaacattta ctcccgattg
    10981 ttcgacagcc cgtttgacct ctcttacaac cgtggccgct ccgacttcga ccgccggcac
    11041 gccttcgtag cgtcctgggt ctggtcgccg gaaatgcggt ttgagagcgg aatgctgaac
    11101 gcgattctgg cgaactggac gctcaccggc atccatacac tgcaaagcgg tctcccgatg
    11161 acgttcgcga tgggagacga cgtcgctctc gacggcaccg gcgggcggca gcacgcccaa
    11221 cttaccggcg atccgattgc tcgggagcat cccagccgcg gcgacatgat cgcgcagttc
    11281 ttcaacaccg gcgcgttcgt tccgacggcc ctggttccga gaggcattta cggcaactcg
    11341 ggacgcaaca tcctgagcgg gcccggcatg gcgaacaacg acatgtcggt gatgaaggac
    11401 atcccggtct cggaacggaa ccggctccag ttccgtgccg agttcttcaa cgccttcaat
    11461 caggtccgac tctcgaaccc ggacagcacg gtcaactcgc agaacttcgg acgaatccgc
    11521 agtgccggat cgccccgggt catccagttc gccctgaaat tcctctggtg agtcccaggc
    11581 cgccccgaag agcgcggcgg cacagccgtc cgtagttggc ggcgaagccg gctacggagg
    11641 gttgtgatgt gtcaaaaccg agattcccct gagcggagca attgacgtcg ggcagtgggt
    11701 tgacaaacca cgctggaccc ggacggatcc ttgttgtact tcctttctgc cgacctggat
    11761 gggccaattt gcgtctgggc tcagcggttg aggccggcag tgaaaacgcc ggttgggaaa
    11821 cccttccccc ttcccggttt ggcgacccca ggctctttcg ggttctctcg atcgcaccgt
    11881 ccaccgcgag ctcgcaatcg ccacggacgg cctccttctg ggccgcgcca gttctgtggg
    11941 caacgtttat ttgctgacga ttcatccacg ctgatccacg ctctttaccg aagctggaga
    12001 gtggccggta tccggcacgg gcggcgttgg taagagcacg gcagccgtga acctgggagg
    12061 gtgcttgttc gacacggatt cccagtcccc gaccgaacaa acctacctgg cgcttgcagg
    12121 acgcctgcac agcacggcag ggatgtgggc gtcacaatga tccgccaatt gcgtctgacc
    12181 gtgctggcgg acaactcggt ggccgccccg aatcttcttg cggagcacgg gctgtcgatt
    12241 ctgattgaag ccgacggccg gcgcattctt ttcgacactg gccaaggcaa agtgctcagc
    12301 gccaacgccg acagcctggg tgccggcctc tctgctctcg acgccgtcgt gctcagtcac
    12361 ggccacttcg accatagcgg aggattggcc agcttgcccg agggcgacgg ccccgctgcg
    12421 gtcttcctcc atcccgccgc gctgcagtcc aagtacgcaa cgagcgacac accgccacat
    12481 cgctcgattg gaatgccgga gcactcacgc caggcgctcg accggttctg gaagcggatc
    12541 gtctggactc aaggtgccac ggaggtggtt cccggcgtct ggtgtaccgg agaaatcccg
    12601 cgtctggcgc cgccggaccc gcgaagcacg ccggtgtttt tctgcgatgc ggactgcacg
    12661 aagcccgatc cgatcccgga cgaccaagcg ctcttcatcg aaatgcaaga cgggctggtg
    12721 gtgattgcgg gctgcgccca ctccggcgtg atcaacacgc tcgaccgcat cggcacgctg
    12781 gcgcggcagg ccgatcttct ggcgctgatc ggaggatttc atctcggccg agcttcatac
    12841 aaacagcttg aggagtgcgg aaacgcaatc gggcgccgca actgtcagtt tctggctcct
    12901 tgccattgca ccgggatgtc cgcgcatgcc tatttgaggt cccgcttcca ttcgctggtg
    12961 caggacgtgg gggtgggaac cagactgatc atcggccatc aatgaaaaga atcagaatcc
    13021 agatcgtcgg tgtgatcgag acaccatttc gggaaccggc tgggtgcccg atccagccct
    13081 cgcgcgcggg cggcgccgaa ggtacggtgc gtgttgaccc gcgttttgcg gagggcttga
    13141 aggatatcga aggattcgag cgcatttggc tgacctattg gctgcatcgg gcgtcatgct
    13201 ccaccctact ggttactccc tttctcgata aacaacagcg tgggatcttc gccacgcgcg
    13261 cgccatcgcg tccttccccc gttggcttgt ccgtggtcag actcctgggc gtcgaaggca
    13321 atatcctcca cgttgcggat gtcgacatga tcgacggaac gccactgctc gacattaagc
    13381 cgtacgttcc cgagttcgat tcttacccgc acagtcgggc cggatggttc gacgattcag
    13441 gttcccaacg ttgcttggcg gacgatcgct tcgaccgatg acccgccgct tgctcggctg
    13501 catgccgcgc acgtccgaag ctgccgatgt cagtgggctt tcttgcgtgg cgagcatgcg
    13561 ttatgtttct ccctggcagg ctgtttcaag agccatcggc tcgccgaact cgccagcagg
    13621 catagacccg ggttcagtag gtggaatcta catctattcc gatgtagtct atgagatgga
    13681 ggaattttgt gcgggcaaag aaaggggcgg aagagtacgt gccgagacga gtatgcctgc
    13741 gccatggcaa gggtcatcct tgcacttgcg cgatggggaa cctctaccgc ttcgtcgagc
    13801 cagtggctct gctgcttctc aagaagaagg gccggtcgta tggctacgac ctggcgggcg
    13861 atcttcggga atacaccctt acggacgccg agatagagag ggccgctctc tatcggacgc
    13921 tgcgccaatt ggagacgaat gggaacgtct cttccgagtg ggacaccgac ggcggtggcc
    13981 ctgcccgacg cgtctaccgg cttacgcctg gtggcgagcg gcacctcgaa gaatggacca
    14041 gtgtgctgga ccacgtctcg aaatcaatgg cgcacttcgt gaaggatgct cgagagtcgt
    14101 tgagcaaccc gatcaaacca gaaccgaatc atgttgactg ctgagtggaa cgagtcgctg
    14161 atctcgaaag cgatcattga aagctaccat caaaagctac tgcgctctgt cgagagcaat
    14221 attgtcatcg tcggggccgg tccagccggc cttaccgcgg cgagcctgct cgcggcgcaa
    14281 ggccgcaagg tgacggtgct tgagaagcgc cttgcgccgg gcggcggtgt gtggggcggt
    14341 ggcatgggaa tgaatcaagt tgtagtgcag gaggaggcgg ttccgcttct cgatgcagca
    14401 ggtgttcgcc acagccgttg cggcaacctg tacgtcgttg acgccgtgga actggcctgt
    14461 accttgtgcc tgcaagcgct ccatgccggt gcagtcttgc tgaatctaac cacgcttgag
    14521 gacctgtgcg tccaacggga caaagttacg ggcgtcgtcg tcaaccgcac aaccatctcc
    14581 ggcgctttac cagtcgatcc gatcgtgcta cgagcggacg tggtaatcga cggatccggc
    14641 catgaggccg tagccgtgga ccgccttcgg gctcgaaacc tgttgccccc gcatttgact
    14701 tcacgttcct gcgaagggcc gatggatgcc gccgccggtg aggcatttgt cgttgaacac
    14761 gcgggcgagg tctattcggg cctgtgggtg acgggcatgg ccgtttgcgc caccttcggc
    14821 ggtccgagga tgggcccgat tttcggagga atgctgctgt cgggaaaaag ggttgcggag
    14881 cggatactag cccaatgaaa agaatcggca tccttggcgg tatgagccca gaatccacgg
    14941 cgctctacta cgagcacatt acccggacgt acaccgcgca gttcggagac tacgggtatc
    15001 cggaaattct gatctatagc gtcagcttcc agcattatgt cgaatggcag cgaaatggcc
    15061 aatggaagga ggctgcggag gaaatggctt cggctcttga acggttgcgc ctggccggcg
    15121 ccgattttgg gttgattgcg acgaacacca tgcactacgt cttcgaccag gtgcagcaag
    15181 cggttggcat gccgctcctg agtatcgtgg acgccacggt agaagcaatt ctggcggctg
    15241 ggctccagac aattgggctc ctcggaacca cgttcaccat gcgcgagcgc ttctttctgg
    15301 acggcctgga gcgtgcgggc atctgcgcat tagtgcctga cgtgtgcgat caggttcgcg
    15361 tacacgacgt gattttccag gaactttgcc gcggagaagt ccggcccgaa tcacagcagt
    15421 tctacatgga catcgtcgat caactgcgcg gtcgcggcgc cgaaggaatc atccttggct
    15481 gcaccgaaat tccactattg ctgaagcccg agcaatgcga tgtgccgcta ttcaacacgg
    15541 cactgctaca cgctgacaag gcgcttcgcc ttgcgcttca accggagcac tgaaatgaag
    15601 gatcgaacgg tacggatcgg gatcatcatt tgcgcacggt atcgcgacta cggcggcgga
    15661 aaatgcttcc gggcattgcg ccaacgccag ggagcatttt cacgctacgc ccccgaggaa
    15721 tgcgtggaaa tcgtcgggta ttccacctgt ggcggctgtc cgggaggcaa cgtcgagtac
    15781 gtacccgccg agttcgtgaa gaatgacgcg aacgtcgttc acttagcgac cggactcgtg
    15841 gtcggctatc ctccttgccc ccacattcac cagttcaagg cttttatcga aaagcggtac
    15901 gggctgacgg tagtcgtcgg gacgcatccg attcctctga aatacctcca gacccacaat
    15961 cagcttccct tctgggaaga gagcggcatg gccgacattg ccagccatct cctcgccgag
    16021 cctcgcgacg tgatggaggc gtacaactag cgagtttggt gcagaatcaa aaactcctgc
    16081 ctgggaagga aattgtgctg cacgaggagt ttgaaaccgg ctcgctccaa ctccgccttg
    16141 atttcgtctg ccgtgaaacg gtagtgatcc ggcggcccgc caggggcgcc cttcttaaaa
    16201 tcgatgatgg cgagacgtcc accgggcttc agagattttc cgaggatccg gaagtacgct
    16261 tcccgttggg ggatgtgatg ataggtgttg acgatcagca cgcagtccac cggctcgggc
    16321 aggttcggcg aggtgctgga cgcccggatc gctgtcacgt tcgtcaggcc ctctttctcc
    16381 gcacgctgct tcaaatgctc caccatcgca ggttcgatat cgacggcata cactttgggg
    16441 gccgaggcat cgcgcgctaa tcgggacata aagtaccccg tgcctgctcc aatgtcggca
    16501 accacctgtc cgggtttcag gttcagggcc gccagaactt ggtctctcgt catattgaaa
    16561 cgcaacgaca ttgacggctt gccgctgaac agccgcgggg tacagggagc gctcgacttc
    16621 gtacccgggc tgctgttcac gccggcgagc cgcggcgaag cgggccagtt taccgccaat
    16681 ggccagcgcc cgaatacaaa ctatttcacc gttgacggag tcagcgcgaa caacggcatg
    16741 agcggcagtg ggttaccggg agacttttcg ggcggcgcgc tcccgggtat gacggcgatt
    16801 ggcagccttc acggcttggt cacatctttg gctttggaag aggtgcgtgt ccaaacctca
    16861 acctttgcgc cggaattcgg caggttgcct ggagcccaga tcgcagtaat cacacgctcc
    16921 ggttcgaaca attttcatgg tgaatttttc ggagcgctcc gccgcggcag gttgaatgca
    16981 ggcgactggt tcgcgaatcg ggcgggactc gatcggagtg gagcggatgc agccactgcc
    17041 ggtggtcttt tccggacctg tcctccggaa caggattttt tttgccgccg cagtcgaacg
    17101 attgcatctg aatcagagtg ctgcctggcg tatggctgtg ccttcgatcg aagaacgccg
    17161 cgatgcgcct gtcgcgcgca ggccggttct ggatgcgttc ccgctgccgt catctccgtt
    17221 ggaggccgag cataccgctc aaacccggtg gccgggagac gttctgacga acagcgtccg
    17281 cgtggatcat gccttctcct caaacggact ggcgttcctg cggtgaactc gaacgccctc
    17341 gaacagccgg ttcggatact tgcaggtgaa cgaagcgcaa ctcgattcca ctggagtgac
    17401 cgcgggactg ctgatgtctt ccggcccccg cctcaccaat gacgcgcgga tcaacctctc
    17461 cagagccacg gcggattcgc gatggagcgc gtccgggctc ggtggcgcac agccgctgga
    17521 tcctgcgagt gtgcttcctc ctttatccgg caccggacgc ggcgtttatg cattgtcgat
    17581 agcgggatta gggccgcttc ttgccgcgga gccggcacga aaccggcaga cggagttaaa
    17641 cctcatcgat accatcgccg ttcattccgg caatcaccag gcaaagttcg gcatagatta
    17701 tcaccgcctg gtctccacgc gcgcggagcc tatctccggc gtcatcgcat cgtacaccag
    17761 tctgccggac tttatcggag gagccgaacc ggcggtcgat tttttacagg cggccagtgg
    17821 ttcgagcgtg atcgaaacgt tttctctgtt cgcgcaggac acgtggcagt tgggaaaccg
    17881 gctgaatttt accttcggca cccgttggga gattacaccg gctccaacgt accggagccc
    17941 gaacggaacc tccgccatcc ggctgatctc gggagagata cccccacgcg aaaggcccga
    18001 tcccggagag cccactccat ttccggccag tacggcggcc gttccttcgt ggaaaacccg
    18061 ctatggccag tttgcccccc gcgccggcgt cgcgttacga ctggatgagg agggctcgct
    18121 gattttccgc gcgggcgccg ggcttttcta tgacctcgga ttcagttcag tagccgatgt
    18181 actgaacgga gtgccgttca atcgttggat tgtcaatctc acggctggag ctccgggttc
    18241 cgggcagcgg gaggtcctct atggatttgc agaggacctg aagcttccgt attccgtgca
    18301 ctggaacatc gcgatcgaaa aactgctgaa ccgccgctcg gcggtatccg cggcttacgt
    18361 gggatcgccg gcacgccggt tgttgcgcct ggagcgccgt ccggccgccg tagcagatca
    18421 accaggatct gttctggcaa gcaatggagg atcttctcag tatcactccc ttcaactcca
    18481 ggcccgcagg tctgtcgccc ccggtctgaa cgggtttgcc gcttacactt ggggacattc
    18541 gatcgacaat gcttcctgga actcggccat cttcttcctc tatccgggcg cgacagaccg
    18601 cggttcgtcc gacttcgatg tccgccactc gtttcaaacc ggactttcct ataacctcaa
    18661 ttccagcgca atgggacgtt ggacgcgtgg atggacggta agcaccattt tccggacgcg
    18721 caccggcttc ctgattgacg tcgtggcccg ggagaatccg ttcggattag gatccgacaa
    18781 cgcgcggccg gatctggtgg acggcgttcc cgtctggatc cgggatccat cagtgcccgc
    18841 cggccgccgt ctgaaccggg atgcgttccg gattcctgca cccggtctgc aaggaagcct
    18901 tggacgcaat gcagtgcgcg ggttcggact ggctcagatc gacgcctccc tggagcgccg
    18961 cttcaccatc ggagaagcgg ccgccctggg aattcgcctg gaggcgtaca acgtcacgaa
    19021 ccgcccgaat ttcgccgatc cgatgcggtt gctgaacaac ccgctcttcg gccaatctct
    19081 ctcgcaggca aacctcatgc tcggcacagg ccgtgcgcat agcggcttga ctccgaactt
    19141 tcaatccggc ggaccgcgat cgcttcagtt acgaattgat gtgtaattct gaatcgaagg
    19201 ctacaggcca agccggagaa tccggcccga tagctccagc acgtacagcg tgttggactg
    19261 tccgtccaaa gccatgctga cgggtccctg aagatcgtct gcgacaacct ccgcttccgg
    19321 tgtatcgaac cgcaaaagcc ggcccggcgc ggctggttgt gcactctggt tggaactgaa
    19381 ctccagcgca aaaaactgag cccgcgatgt gcccatatcc cgccacaaca catcgacaac
    19441 ggacgtgcgg ccaaacatga agggctggct ggaacgctgg tccggatcga ctatgaggac
    19501 tcgcgccgtt ccgggtatga aaggaaatcc tgtcaggaaa ctgacgagca gttcgttccc
    19561 ataagttcgc acattcgtag gcacagggtc gatcataggc ggtcccacgg cgccagggtt
    19621 ttgcattggg gggaaacgca caatccgttg ccatcgcccc gtttcggggt cgatccgcat
    19681 cagggcattc atcgacgcgt ctacggccca gagaaccgaa ccgtccccac tcgacgccag
    19741 tccccaggga tttgaaaacc ggtagatcgt gaccgggtcc ggttcggaga tcggcaaacg
    19801 cgccagcaca tctactgaca cggaagctcc gctgccgtcg gtgatctgca cttcgccgcc
    19861 gtccataaga ctctgctggt gctcgggtgt catgcgaaag ctaccggtta tctggtcgat
    19921 atcgctgcca aaccgcagcc gcagaatgga agcgagtaac gcggaggagc gtccctctgg
    19981 attatggatg gctgtcccag gagcctcccc tcgcctttcg gcatctcctc cgccgatggc
    20041 gaggtacagc gtacggttgc tcacggccat cgcggtgggg ccacttgggc cgccggcgac
    20101 gtcaagaccc gatggcagac cttcgatcaa cgtccggcgc gcgcctccgg gtgtcacaag
    20161 agagacgcgg cctgcattag gttgtgtgct ggtttccgac accagcagat tgcccccagg
    20221 gctcacgacg attttttgag gcgcctg
//
LOCUS       DASTMK010000272         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_109489, whole genome shotgun sequence.
ACCESSION   DASTMK010000272 DASTMK010000000
VERSION     DASTMK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109489"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFQ79_11905"
     CDS             complement(<1..612)
                     /locus_tag="VFQ79_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140415.1"
                     /translation="MEPHDGVNFPYTPGTNGMVELEHADDTVLTRRDLLKGGGTVAGF
                     LAAANYAAAQQPPASGLMPEAKAGPQLPLADFGKHRITRLIAGANPIYGYSHFNYVFS
                     ATMGEYHTTGRVLAFLKELERAGLNTWQASWSERLETDWTKYKEQGGTMQLIVLSRPD
                     FNEHPEMAARAAKLKPMGIAQHGGATNRLWDAGEFDKSKDYLKR"
     gene            complement(717..2168)
                     /locus_tag="VFQ79_11910"
     CDS             complement(717..2168)
                     /locus_tag="VFQ79_11910"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU0140416.1"
                     /translation="MNHDDRKPTTTDAGIPVASDEHSLTVGPDGPILLQDHYLIEQMA
                     NFNRERIPERQPHAKGGGAFGHFEVTRDVRAFTKAAVFQPGTKTNTLIRFSTVAGERG
                     SPDTWRDPRGFALKFYTTEGNYDMVGNNTPVFFIRDPMKFQHFIRSQKRRADNGLRDH
                     DMQWDFWTLSPESAHQVTWLMGDRGIPKNWRQMNGYSSHTYMWVNAAGERFWVKYHFK
                     TDQGIDFLTQQEADRLAGVDGDYHRRDLFDAIKRGDYPSWTLKMQIMPFEEAKSYRFN
                     PFDLTKVWPHADYPLQEVGRLTLDRNPTDFHTEIEQAAFEPNNLVPGIGISPDKMLIG
                     RLFAYADAHRHRLGVNYKQIPVNQPEAPVHSYSKDGAMRVQNVSDPVYAPNSKGGPKA
                     DGERYPEVATWETSGSFVRDAYTLRKDDDDFGQAGTLVRKVMNDEQRDRLVSNVVGHL
                     KDGVSRPVLQRAFEYWRNVDKEIGDRIAAGVEG"
     gene            complement(2374..3015)
                     /gene="lexA"
                     /locus_tag="VFQ79_11915"
     CDS             complement(2374..3015)
                     /gene="lexA"
                     /locus_tag="VFQ79_11915"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU0140417.1"
                     /translation="MALTKRQKEVIDFIAEYIHTHGYSPSYEEIGKALDLASLATVHK
                     HISALEQKQYLRRSYNQSRSLELAPRYFQELSRSMNMNESTLSIPLLGQIAAGVPVET
                     PAMPDCLILSDFLRNENLYALRVRGESMIDDHICDGDYVLVERAANVKDGDIVVALIE
                     GMETTLKRFFSDGGGRARLQPANSDMRPINVSLEDLEIQGRVLAVLRRYTIVN"
     gene            complement(3167..3403)
                     /locus_tag="VFQ79_11920"
     CDS             complement(3167..3403)
                     /locus_tag="VFQ79_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0140418.1"
                     /translation="MPIREFICNQCGTDFEKIVRATQATSEIACPSCGSHHLEKKLSG
                     FAAHSGGSRAAAPAPMCPSGGVCGTPGMCGMSRN"
     gene            complement(3435..3821)
                     /locus_tag="VFQ79_11925"
     CDS             complement(3435..3821)
                     /locus_tag="VFQ79_11925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU0140419.1"
                     /translation="MREIGVPADAVFLADASGLARLNLVSPQATVVLLNSMWQSPERD
                     AFVGSLPIGGKDGTLENRFEASSLADRIRAKTGTLRGVSALSGYATTRSGKTLSFSVF
                     VNNHLGSGRAARDFLDKIALAIAGPP"
     gene            complement(3746..>4242)
                     /locus_tag="VFQ79_11930"
     CDS             complement(3746..>4242)
                     /locus_tag="VFQ79_11930"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU0140420.1"
                     /translation="LNRVLTAPSPAGPIEWELAPGRLLLLRGSIAVGARPWRGKVAID
                     EPALYAAHYLRDALLRRGIAVSGSARARHRLAGDPEPAAPQLTLLARHASPPVSEIVR
                     SVNKESVNLFAELLLAEVARTSTGINSPERGLAELPVSCGKLEFPPMPFFWPTQAVSH
                     VSIW"
BASE COUNT          797 a         1215 c         1346 g          884 t
ORIGIN      
        1 gcgcttcagg taatctttgg acttgtcgaa ctcgcccgcg tcccacagcc ggttggttgc
       61 tcctccgtgc tgtgcgatgc ccatcggctt gagctttgcg gcccgcgccg ccatctctgg
      121 atgctcattg aagtccggac gcgagagcac gatcaattgc atagtgccgc cctgctcttt
      181 gtatttcgtc caatcggttt ccagccgttc gctccagctt gcctgccagg tattcaagcc
      241 cgcacgctcc aactccttca ggaacgccag tacgcgcccg gtggtgtggt attcgcccat
      301 cgtggcggag aagacatagt tgaaatggga gtagccgtag atcggattcg cgcccgcgat
      361 cagacgtgtg atgcgatgct taccgaagtc ggctaatgga agctgtgggc ccgccttggc
      421 ctccggcatc agtccggaag cgggtggctg ttgtgccgcg gcgtagtttg ccgccgccag
      481 gaagccggca accgttccgc cacccttcaa aaggtctctc cgggtaagca cggtgtcgtc
      541 cgcgtgttcg agttccacca tcccgttggt tccgggggtg taagggaagt ttacgccgtc
      601 atggggttcc atgcccggga gtatatcgaa tcggataggg catttccgca attcatttca
      661 cgatccactc gggggcagcc cggcttgcgc tcgcgggctg tcccgaatgg ttgaggttag
      721 ccttcgacgc ccgcggcgat gcggtcgccg atctccttgt cgacattgcg ccagtattcg
      781 aaggcgcgct gcagcacggg ccgcgacacg ccgtccttga ggtgtccgac gacgttagac
      841 accagccggt cgcgctgctc gtcgttcatg accttgcgaa ccaaggtgcc ggcctgtccg
      901 aagtcatcgt cgtccttgcg cagggtgtag gcgtcgcgaa cgaaggagcc gctggtctcc
      961 caggttgcga cttcggggta acgctccccg tctgccttcg ggccgccctt cgagttcggg
     1021 gcatacacgg ggtcggacac gttctggact cgcatcgcgc cgtccttgct gtagctgtgt
     1081 accggcgcct ctggctggtt taccgggatc tgcttgtagt tgacccccag gcgatgccgg
     1141 tgggcgtccg cgtaggcgaa aaggcggccg atcaacattt tgtccgggct gataccgatc
     1201 cccggcacga ggttgttcgg ttcgaacgcg gcctgctcga tctcggtgtg gaaatcggtc
     1261 gggttgcgat ccagcgtcag gcggccgacc tcctggagcg ggtaatcggc gtgtggccac
     1321 accttggtta gatcaaaggg gttgaagcga tacgacttgg cttcctcgaa gggcatgatc
     1381 tgcattttca gcgtccagct agggtagtcc ccgcgcttga tcgcatcaaa caggtcgcgc
     1441 cgatggtagt cgccatcgac gccggccaag cggtctgcct cctgctgtgt gaggaagtcg
     1501 ataccctggt cagtcttgaa gtggtacttt acccagaaac gctcgccggc cgcgttcacc
     1561 cacatgtagg tgtggctgga atagccgttc atctgccgcc agttcttcgg aatccctcga
     1621 tcccccataa gccaggtgac ctggtgcgcc gactcgggcg atagggtcca gaaatcccac
     1681 tgcatgtcgt ggtcgcgcag gccgttgtcc gcacggcgct tctgggatcg gatgaaatgc
     1741 tggaacttca tcgggtcgcg gatgaagaac acaggcgtgt tgttccccac catgtcgtag
     1801 ttgccctcag tggtatagaa cttgagagcg aacccgcgtg ggtcacgcca agtgtcgggg
     1861 ctgccccgtt ccccggccac cgtggaaaat cggatcaatg tgttggtttt cgtgcccggc
     1921 tggaatacgg cagctttggt gaaggcgcgg acatccctcg tcacctcgaa gtgaccgaag
     1981 gccccgccac ccttcgcgtg cggctggcgc tccggaatcc gctcccggtt gaagttggcc
     2041 atctgctcaa tgagatagtg gtcctgcagc aggatgggac catcgggccc aacggtgagc
     2101 gaatgctcgt cgctggcgac ggggatcccg gcatcggttg ttgtgggttt gcggtcgtcg
     2161 tgattcacgt aacacctccc ggaatgaccc tctaaaccga gacatggaaa tcccagaatt
     2221 tgagggatag cttattctgc caccattggc gcggtgtgtg ggggcagcga atggtgtgcc
     2281 gggtagggct gtcgctatct cgattcgtag ggtccgtgga taccgataat cgcagggtaa
     2341 caatactgtc gcagacgatc gagcgatcat gcgctaattg acgatggtat agcggcggag
     2401 cacggccaac acgcgaccct ggatttcgag atcttcgaga gagacgttga tggggcgcat
     2461 gtccgagttg gcgggttgca ggcgcgcacg gccaccgccg tcggaaaaga aacgtttcag
     2521 cgtcgtctcc atcccttcga tcagggcgac gacgatgtcg ccatccttca cgttcgcagc
     2581 ccgctccacc agcacgtagt cgccatcgca aatgtgatcg tcgatcatcg actcgccacg
     2641 cacccggagg gcgtatagat tctcattcct caaaaagtcg gagagaatca ggcagtctgg
     2701 catcgcgggg gtttcgacgg gaaccccggc cgcgatctgc ccgaggagcg gaatactcag
     2761 ggtgctctca ttcatattca tgctgcgcga gagttcctgg aagtagcgag gcgcgagttc
     2821 gagagagcgg ctctggttgt aggagcgccg cagatactgc ttctgctcaa gagcggaaat
     2881 gtgcttatgc acggtggcga gagatgccag atcaagcgcc ttcccgatct cttcgtagct
     2941 ggggctatat ccatgcgtat ggatgtactc cgcaatgaaa tcgatcacct ctttttgccg
     3001 tttcgtgagt gccatcagat ctcgtccctc ggaaactagt ttttcttacg ataggcgaat
     3061 aaatgccgaa ggtcaagaag aaacgtgcag ccaaacgcaa tcaataacga gatgacggat
     3121 tcaggccata gagatagcca tgcggtggct tattgagggg ataatctcaa ttacggctca
     3181 taccgcacat gccgggcgtt ccacagacgc caccgctggg gcacatcggg gctggagccg
     3241 cagcgcggga accgccactg tgcgccgcga agccggagag tttcttttcg agatgatgac
     3301 tgccacagga agggcaggca atctccgaag ttgcctgcgt cgcccggacg atcttctcaa
     3361 aatccgtgcc gcactggttg cagataaact cgcgaatcgg catcttccaa aacctccgaa
     3421 acgtggcggg agacttaagg cggacccgca atcgctaagg ctatcttatc gaggaagtct
     3481 cgtgctgcgc gaccgcttcc taagtggttg ttgacaaata cgctgaatga cagcgttttg
     3541 cctgagcgcg tggtggcata accggagagc gcgctcactc cgcggagagt gccggtcttg
     3601 gcgcgaatgc ggtctgccag cgagctagcc tcgaagcggt tctcgagggt gccgtccttg
     3661 ccgccgatag ggagtgaacc gacgaaggcg tcgcgctccg gactctgcca catgctgttc
     3721 agcaatacga ccgtagcctg tggactcacc agattgagac gtgcgagacc gcttgcgtcg
     3781 gccagaaaaa cggcatcggc gggaactcca atttcccgca ggaaacgggg agctcggcga
     3841 ggccccgctc gggagagttg atccctgtgc tggtgcgcgc tacctctgcg agaaggagct
     3901 ctgcaaagag gttcacactt tctttattga cgctgcgcac gatttccgag acgggcgggg
     3961 aggcatgacg ggcaagcagg gtgagctggg gggcggcggg ttccggatcg ccagcaaggc
     4021 ggtgacgtgc gcgtgcggag ccggaaacag cgattccacg gcgaaggaga gcgtctcgca
     4081 gatagtgcgc cgcatagagg gccggttcgt cgatggcgac ctttcctcgc cagggccggg
     4141 ctcccacggc gatgctgccg cgcaagagga gcaggcgacc gggtgcaagt tcccattcga
     4201 tgggtccggc gggtgatggc gcagtgagga cgcgattgag ga
//
LOCUS       DASTMK010000273         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_396867, whole genome shotgun sequence.
ACCESSION   DASTMK010000273 DASTMK010000000
VERSION     DASTMK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396867"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFQ79_11935"
     CDS             complement(<1..1138)
                     /locus_tag="VFQ79_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0140421.1"
                     /translation="MADSVNLTIDGKAVQAAPGTLVIDAAKTVGIEIPAFCYWEGLTL
                     QAACRMCLVEVERMPKLMTACTLAVAEGMVVRTETPLVAEARKSMLEFVLTNHPLDCP
                     VCDKGGECELQDMTFRYGAGESRFDEMKVHVDEKQWSPVVYFDAPRCILCYRCVRICD
                     EGMGVDALGVIYRGVVSEIAPNKDDHLECDECGMCIDICPVGALTSGMYRYRTRPWEM
                     EHTGTICAHCSNGCKTTLGTRNGQIIRGNNRDKSGINGEFLCIKGRYAFDFNSHAERL
                     QSPLMRIDGKLEPVSWSRAIEEVARKFTEIRERGGRFGVVGSNRTSNEENYYLQKFAR
                     QGLGTNNIDHRRTGDVPALIDALAGRTGALATVKDVYTAKASLVI"
     gene            complement(1152..2462)
                     /gene="nuoF"
                     /locus_tag="VFQ79_11940"
     CDS             complement(1152..2462)
                     /gene="nuoF"
                     /locus_tag="VFQ79_11940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU0140422.1"
                     /translation="MLYNEPSPLEVKVLSRRFGLPNSQSIGTYLAHEGYRAFRKAAAM
                     TPDDIINEVKISSLRGRGGAGFPTGMKWGFVPRNSPKPKYIVVNADESEPGTCKDRLL
                     MEYDPHQLLEGMMIAGLAVDAHAGYIYIRGEYRYLIDLMDRAIAEAYERGYLGKNIQG
                     TGFDFDVYTHTGAGAYECGEESALLESLEGKRGIPRIRPPFPAVSGAFQCPTVLNNVE
                     TYCAVPAIIRDGGAAFAALGTPKNGGTRLFCLSGHVNRPGVYELPMGFNLLRMINEVG
                     GGVRGGKKLKAVIPGGSSCPVLKAEECDLAMDFDTVAKAKSMLGSGGVVVMDEDTCMV
                     KAALRIMKFYAHESCGWCIPCREGTIWLRKLLDRFHAGFGQRSDIPLIGEVAKNMLGR
                     TFCPLGDAAAMPTISFVEKFYDEFEAHLDGKPCPYEEVNRFALA"
     gene            complement(2474..2956)
                     /locus_tag="VFQ79_11945"
     CDS             complement(2474..2956)
                     /locus_tag="VFQ79_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU0140423.1"
                     /translation="MTFSPELEAKFEKLLRSYPPGRQRSALIPMLMFAQDEVGSVTPE
                     VIAEIAARIGISELQVREVLSYYSMLHTKPMGKYHVQVCTNISCLLTGGEEIWEQAKK
                     KLGCGHKEVTADGQFSLEEVECIGACSWAPAIQVNYDFHHNVTPEKFNALIDSLRKLH
                     "
     gene            complement(2957..>3970)
                     /gene="nuoD"
                     /locus_tag="VFQ79_11950"
     CDS             complement(2957..>3970)
                     /gene="nuoD"
                     /locus_tag="VFQ79_11950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU0140424.1"
                     /translation="KTYQQVVPLTDRVDYLANLSNNLGYSLAVEKLLQLEIPSKAQWM
                     RVLLVELTRLNSHLVWLGTHALDIGAMTVFLYCFREREDLLRIFEMFSGQRMMTSYIR
                     VGGLALEPPRGWEEAVGKFIDAFPSKLAEYEDLLTKNPIWMNRTKGVGFLALEDLLDC
                     GVTGPMIRAAGMKWDIRKDEPYSSYDKFDFEIPTREASDVYARYEVRLEEMRQSVRII
                     RQAMDGMPEGAWVTNAPNVVLPEREKMKTQIEALIYHFKIVTEGFTVPAGEAYVCIES
                     PRGELGYYVVSNGTSRPHRVFMRTPSFANLQALPDMVKGRLIADTIAALGSIDFVLGD
                     VDR"
BASE COUNT          808 a         1247 c         1067 g          848 t
ORIGIN      
        1 cgatcaccag gcttgccttg gccgtgtaca catctttcac ggtggccagc gcgccggtgc
       61 ggccggccag tgcatcgata agcgcaggga cgtcgccagt gcggcgatgg tcgatgttgt
      121 tggttcccag cccttgccgc gcgaacttct ggagatagta gttctcttcg ttgctggttc
      181 ggttagatcc cacgacaccg aaccggccgc cacgctcccg gatttccgtg aacttccgcg
      241 cgacttcctc gatggcgcga gaccaggaaa ccggctccag ctttccatcg attcgcatca
      301 gcggagactg aaggcgttcg gcgtgtgagt tgaaatcgaa ggcataccgg cccttgatac
      361 agaggaactc gccgttgatg cccgatttgt cgcgattgtt gccccgtatg atctgcccgt
      421 tgcgggttcc cagcgttgtc ttgcatccgt tggagcaatg agcgcaaatg gtcccggtgt
      481 gctccatctc ccaggggcgg gtgcgataac gatacatgcc gctggtcagt gcacccaccg
      541 ggcagatatc gatgcacatc ccgcactcgt cgcattcgag atgatcgtct ttgttcggcg
      601 cgatctcgga aaccacgccc cgatagatga cgcccagtgc gtccacaccc attccttcat
      661 cgcaaatccg gacgcagcga tagcagagaa tacagcgagg tgcatcgaag tagacgacgg
      721 gcgaccactg cttttcgtcg acgtggactt tcatctcgtc gaagcgactc tcgccggctc
      781 cgtaccggaa agtcatgtcc tgcaactcgc actctccgcc cttgtcgcag accgggcagt
      841 cgagcggatg attggtgagg acaaactcaa gcatcgactt ccgcgcctcc gccaccaggg
      901 gcgtttccgt ccgcaccacc atgccttcag cgacggccag cgtgcatgcc gtcatgagct
      961 tgggcatccg ttccacttcc accaggcaca tccggcaggc agcctgaagc gtaaggcctt
     1021 cccaataaca gaacgccggg atctcgatgc ctaccgtttt ggcggcgtca atcaccagag
     1081 tcccaggtgc ggcctgcacg gccttgccat cgatcgtgag atttactgaa tcagccattt
     1141 atacaaaagc actaagccag cgcgaaacgg ttcacctctt cataaggaca cggcttgcca
     1201 tccagatgcg cttcgaactc gtcgtaaaac ttctccacaa aggaaatggt cggcatggct
     1261 gccgcgtcgc ccaacgggca gaacgtccgg cccagcatat ttttggcgac ctcgccaatg
     1321 agcggtatat cgctccgttg tccaaagccc gcatggaaac ggtccagcaa cttccgcagc
     1381 cagatcgtac cttcccggca cggaatgcac cagccgcagc tttcgtgggc gtagaacttc
     1441 atgatgcgga gtgcggcttt caccatgcac gtgtcttcat ccattacgac gacgccgccc
     1501 gagccgagca tgctcttggc cttggcgacc gtatcgaaat ccatcgcgag gtcgcattct
     1561 tcagccttca gcaccgggca ggaagacccg ccgggaataa cggccttcaa ttttttgccg
     1621 ccgcggacac cgccgcccac ttcgttaatc atcctcagga gattgaagcc catcggcagc
     1681 tcgtagacgc cgggccggtt tacatggccc gagaggcaga acagccgtgt cccgccgttt
     1741 ttgggcgttc ccagcgccgc gaaggctgcg cctccgtcgc gaataatggc cggaacggcg
     1801 caatacgtct ccacgttatt cagaacggtg ggacattgga aggctccgga cacggccgga
     1861 aacggcggac gaatacgggg aatcccccgc ttcccctcga gagattcgag caaagccgac
     1921 tcttcgccgc actcatacgc tccggcgccg gtgtgagtgt aaacatcgaa gtcgaagccc
     1981 gtaccctgga tgttcttgcc aaggtagccg cgttcgtagg cttcggcgat agcccggtcc
     2041 atgagatcga tcagataccg gtattcgccg cgtatataaa tatagccggc atgcgcatcc
     2101 actgccagcc cggcgatcat catgccttcg agcaactggt gcgggtcgta ttccatcagc
     2161 aggcggtctt tgcaggtgcc gggttcgctt tcgtcggcat tgaccacgat gtacttcggc
     2221 ttcggcgaat tccgcgggac aaagccccac ttcatcccgg ttggaaagcc cgctccgcca
     2281 cgtccgcgaa gactggatat cttcacctca ttgatgatgt cgtccggcgt catcgccgcc
     2341 gctttccgga atgcccgata cccttcgtgc gccagataag tgccgatcga ttgggagttc
     2401 ggcaggccga accgccggga cagaactttg acttcgagcg ggcttggttc gttgtaaagc
     2461 atctcttcaa gaactagtgc agttttcgca ggctgtcgat cagcgcgttg aatttctccg
     2521 gagttacgtt gtgatggaag tcgtaattca cctgaatagc aggggcccag gagcacgcac
     2581 cgatgcactc cacctcctcc agcgaaaact gcccgtctgc cgtcacttct ttatggccac
     2641 agccgagttt tttctttgcc tgctcccaga tctcctcgcc accggtcagc aggcaactga
     2701 tgttggtgca aacctggacg tgatacttcc ccatgggctt cgtgtgaagc atggagtaat
     2761 agctcaaaac ctctctcacc tgtagctcgc tgattccaat gcgtgccgcg atttccgcga
     2821 tcacttcggg tgtgaccgag cccacttcgt cctgggcgaa catcagcatc ggaatcagcg
     2881 ccgaccgctg tcttccgggc gggtaactcc tcaacagctt ttcgaacttt gcttccaact
     2941 caggcgaaaa agtcatttac cggtccacat cgccaagaac gaaatcgatg ctgcccaatg
     3001 cggcaatggt atcggcgatc agcctacctt tcaccatatc cggcagcgcc tgcaaattcg
     3061 cgaaactcgg cgttcgcata aacacacgat gcggccgcga agtcccgttg ctaaccacgt
     3121 agtagcctaa ctcgccgcgc ggagattcga tgcacacata cgcctctccg gcaggtaccg
     3181 tgaaaccctc cgtcacgatc ttgaaatgat aaatgagggc ttcaatctgg gtcttcatct
     3241 tctcgcgctc cgggagtacc acgttcggag cattggtcac ccaggcgccc tccggcattc
     3301 cgtccattgc ctgccggata atccgcacgc tctggcgcat ctcctccagg cggacttcgt
     3361 accgggcgta aacatcgctc gcctcacgcg tgggaatctc aaaatcgaac ttgtcgtagc
     3421 tcgaatacgg ctcgtctttg cggatatccc atttcatgcc ggcggcgcgg atcatcggac
     3481 cggtgacacc gcaatccagc agatcttcga gcgcgagaaa tcctacgccc ttggtccggt
     3541 tcatccagat cggatttttt gtcagcaggt cttcatattc ggccaatttc gacggaaatg
     3601 cgtcaatgaa cttaccaacc gcttcttccc acccgcgcgg cggctccaac gcaagtccgc
     3661 ccacgcggat gtagctggtc atcatgcgct gcccggaaaa catttcgaaa atccgcagga
     3721 ggtcttcgcg ttcgcggaaa caatagagaa acaccgtcat ggcgccgatg tccagggcgt
     3781 gcgttcccag ccagaccagg tggctgttaa gccgcgtcag ctccaccagc aggacgcgca
     3841 tccattgcgc cttcgacgga atctccagtt gcagcagttt ttcgacggcc agcgaatagc
     3901 ccaggttatt cgacaggttg gcgagatagt cgacgcggtc ggttagcggc acgacctgct
     3961 gatacgtctt
//
LOCUS       DASTMK010000274         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_396880, whole genome shotgun sequence.
ACCESSION   DASTMK010000274 DASTMK010000000
VERSION     DASTMK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396880"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFQ79_11955"
     CDS             complement(<1..766)
                     /locus_tag="VFQ79_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU0140425.1"
                     /translation="MVKAARWIVPAPAEGEIARLAQALNIRPPAARVLFSRGYTNPSE
                     ANRFLRPSLDDLCDPFLMTDMARAVERLLSAVRNREKILLYGDYDVDGTASIVVLKKA
                     IELAGGEATYHVPHRLRDGYGMRPEVIDRAASEGIRLIVSVDTGIRAGDVVARAEALG
                     VDVIVTDHHLPEAELPRALAVLNPNRRDCSYPEKNLCGAGVAFKLVQGLFSRLGWPAG
                     RIQRLNESFLKLVAIATIADVVPLTGENRIIVRHGLE"
     gene            complement(797..1285)
                     /locus_tag="VFQ79_11960"
     CDS             complement(797..1285)
                     /locus_tag="VFQ79_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140426.1"
                     /translation="MKQNLDALKTDIVDELNRRNFVVFYAATGPEPSPVVHWDTRGRP
                     DFKEFIDVAARLGISLIVFHHWQFTANQVDSVIDDLDAADLAVDEARTYRRRLEDLRD
                     YEGFTCVVELSFQAQGRDFMFHLHTDWFAEFLSISSDIDSAFADDEDDEEDPMGGYFS
                     RN"
     gene            complement(1282..3147)
                     /locus_tag="VFQ79_11965"
     CDS             complement(1282..3147)
                     /locus_tag="VFQ79_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0140427.1"
                     /translation="MIHDQDLIAIQEVRGKVEAAYAAWQKYRAYSQAQIDAIVEAMGA
                     VGRQHALDLARMAVEETGYGNVKDKLAKNLLCADLLPRSIRNMKTVGVLREIPEQKVV
                     EIGVPVGVVAAIVPTTNPTSTVIYKSIISLKAGNGIVLSPHPRAQKCTCHTADLMHRA
                     AVEAGAPEGIIQCLGKANMDATKALMTHKRTGVILSTGGSGIVRAAYSSGKPAFGVGP
                     GNVPVLLEKTADIPQAIANVVAGKAFDYGTVCSSEQALVAGRDLREQVLKQLKVQKAY
                     LCNEQQKQALGKLLITPNWTVNPKCVGQAPTTIARMAGFEVPEDTSILAVELDGVGKE
                     HPLSAEKLSPVLSLYFVADFNAAMDTCEAVLRFGGLGHTCVIYSKDEERIRDFGMRMP
                     AMRVLVNTSAPQGSTGITTGILPSMTLGCGAMAGNITGDNVGPLHLINTKRIAYAIRK
                     AEDVLRVPDVPQEVSSPVSASGSLASVDRSTVIAAVEKYLEQRGFPVQSSRMAPGPER
                     EAPAAPVTASNISNVAADVVERFLKRRSAPAQKSARANCGCADSCPAPPAAQTTPEPT
                     IPQPEVRIVDFVCEADVRAAVQQAKKIFIGPKTIVTPAARELAAQHDTLVMAERS"
     gene            complement(3203..3496)
                     /gene="pduA"
                     /locus_tag="VFQ79_11970"
     CDS             complement(3203..3496)
                     /gene="pduA"
                     /locus_tag="VFQ79_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HEU0140428.1"
                     /translation="MEALGMVETKGLVGAIEAADAMVKTANVQLTGKEFIGAGFVTVT
                     VRGDVGAVKAATDAGAAAARRVGELVSVHVIPNPHDEVEKILPGQKKGEKPLG"
     gene            3634..>3920
                     /locus_tag="VFQ79_11975"
     CDS             3634..>3920
                     /locus_tag="VFQ79_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140429.1"
                     /translation="MALQKSSLIHTAVACLVIAVPARADLKPETNRAFEQYVAKAEQR
                     IARRTNGGARFLWVDESPARMAEVRRGGVAAGSNIANTEVEVPDGLIHDWTG"
BASE COUNT          780 a         1219 c         1142 g          779 t
ORIGIN      
        1 cctccaatcc gtggcgcaca atgatgcgat tctcgcccgt caaaggaacc acgtcggcga
       61 tggtggcgat tgccaccagt ttcaggaacg actcgttcag ccgctgaatt ctcccggcgg
      121 gccatcccag tctgctgaac agcccctgca ccaacttgaa cgccacgcct gctccgcaga
      181 gattcttctc cggataagaa cagtcgcggc gattcggatt gaggacagcg agcgcacgcg
      241 gcagctcggc ttcgggcagg tgatggtccg taacaatcac gtcgacgcca agcgcctccg
      301 cgcgtgcgac cacatctccc gcccggatgc ctgtatcgac gctgacgatc agccgtatgc
      361 cttcgcttgc ggcccggtcg atcacttcgg gacgcatccc gtagccgtcg cgcagccggt
      421 gcggcacatg gtaagtggcc tcgccgccgg caagctcaat ggccttcttc agaacgacga
      481 tggaagctgt cccgtctacg tcgtaatcgc catagagcag aatcttttcg cgattccgca
      541 cggcactcag caaacgctcg actgcgcgcg ccatgtccgt catcaggaaa ggatcgcaga
      601 gatcatcgag cgaaggccgt aaaaaccggt tcgcttctga ggggttcgtg tagccgcggc
      661 tgaagagcac tcgtgcagcg ggaggccgga tgttcaaagc ctgggcgagc cgcgcgatct
      721 cgccctccgc gggggcaggc acaatccagc gggcggcctt caccatcgcg gtggctgcca
      781 gtggaatctc acagtttcaa ttcctcgaga agtatccgcc catcgggtct tcttcgtcgt
      841 cctcgtcgtc ggcgaaggcg gaatcgatat cactcgatat ggagaggaac tcggcgaacc
      901 agtccgtgtg gagatggaac ataaaatcgc gcccctgcgc ctggaatgac aactcgacga
      961 cgcaggtgaa cccttcgtag tcccgcaagt cctccagccg ccgccggtag gtgcgcgcct
     1021 catccacggc gagatcggcc gcatccaaat cgtcaatcac ggaatccacc tggtttgcgg
     1081 tgaactgcca gtgatgaaaa acgatgagcg aaatccccag ccgcgcagct acatcgataa
     1141 actctttgaa atccggacgc ccgcgagtgt cccaatgcac aacgggcgat ggctcgggtc
     1201 ccgtggcggc gtaaaatacg acgaaattac gccggttcag ttcgtccacg atgtcggtct
     1261 tgagcgcatc gaggttctgt ttcatgatct ctccgccatc accagtgtgt catgctgggc
     1321 cgccagttcc cgtgccgccg gtgtgacgat agtcttcggt ccgatgaaga ttttcttcgc
     1381 ctgctgcaca gcggcgcgaa cgtccgcttc acagacgaaa tcgacgatcc ggacctccgg
     1441 ctgagggata gtgggttccg gcgtagtttg tgccgcgggc ggtgcgggac aggaatccgc
     1501 acagccgcag tttgcacggg ctgatttctg tgccggggca cttcgccttt ttaagaaccg
     1561 ctcgacgaca tcggccgcca cgttgctgat gttcgacgcc gtcacgggcg cggcgggcgc
     1621 ctcgcgctcg ggccccggcg ccatcctgct gctttgcact ggaaagcctc gttgctccag
     1681 atatttctca accgccgcga tcaccgtaga ccggtcgacg gaagccaggg aaccggaagc
     1741 ggaaacgggc gaactcactt cctgcggaac gtcggggacg cgcagaacgt cttcagcctt
     1801 tcgaatcgcg tacgcgatcc gcttcgtatt gatcaggtgc agcgggccaa cattatcacc
     1861 ggtgatgttg ccggccatcg ctccacagcc aagtgtcatc gaaggtagaa tccccgtggt
     1921 gatgcccgtg gagccttgcg gagcggaagt attcaccagg acgcgcatcg cgggcatccg
     1981 cattccgaaa tcgcgtatgc gctcctcgtc tttcgaatag atgacgcaag tgtgtcccaa
     2041 tccgccgaag cgcaggaccg cctcacaggt gtccatcgcc gcgttgaagt ccgcgacaaa
     2101 atacaacgac aggaccggcg agagtttctc cgccgaaagc ggatgctcct tacccacccc
     2161 gtccaattcc acggcgagga tagaagtgtc ttccgggact tcaaagcccg ccattctcgc
     2221 aatggtagtg ggcgcctgtc ccacgcattt cggattgacg gtccagttcg gagtgatcag
     2281 gagtttaccc agcgcctgct tctgctgctc gttgcagaga tacgccttct gcaccttgag
     2341 ctgtttcaga acctgctcgc gcaaatcccg gccggcgacc agtgcctgtt cgcttgagca
     2401 gaccgttcca taatcgaaag ccttgccggc aaccacgttg gcgatagcct gaggaatgtc
     2461 ggcggttttt tcgagaagca ccggaacatt gccgggacca acgccgaacg ccggtttccc
     2521 gctggagtac gccgcccgca cgatgccgct gccaccggtg gacagaatca caccggtgcg
     2581 cttatgggtc atcagagcct tggtagcgtc catgttcgct tttccaagac actggatgat
     2641 gccctccggc gcgccagcct caaccgccgc gcggtgcatc agatccgcgg tgtggcaggt
     2701 acacttctgc gccctggggt gcgggctcag gacgatgccg ttgccggctt tgagggaaat
     2761 gatggacttg tagatgaccg tcgaggtggg gttcgtggta ggaacgatcg cggcgacgac
     2821 gccgaccggt acgccgatct caacgacctt ctgctcggga atctcgcgca gaactccgac
     2881 ggttttcatg ttgcggatgg agcgcggcag caagtccgca cagagaaggt tcttggcgag
     2941 tttgtccttg acgttcccgt acccggtttc ttcgaccgcc attcgggcca aatccaaagc
     3001 atgctgccgg ccgacggcac ccatggcctc gacgatagcg tcaatctgag cctgcgagta
     3061 tgcccggtac ttctgccatg cggcatacgc agcttccacc ttgccgcgaa cctcttgtat
     3121 cgcgatgagg tcttgatcgt ggatcattac cggatctgac tacaacatct gacgcaggcg
     3181 ccggatcttg tgcccgggcc gatcaaccga gcggcttttc tccctttttc tggccaggaa
     3241 gaatcttttc gacttcgtcg tggggattag ggatgacatg gacagaaacc agttcaccta
     3301 cacggcgtgc cgcggccgcc ccggcatccg tcgcggcttt caccgcgccg acatcgcccc
     3361 ggaccgttac agtgacgaat ccagcaccga tgaactcctt gcctgtgagc tgaacattgg
     3421 cggttttcac catggcatcg gcggcttcga tagcgcccac cagtcccttg gtttctacca
     3481 ttccgagggc ttccataatc ctcctgtcga atccaactga agctgaaact ctaccgtatc
     3541 acaaaacata aacgctgtac ggaagcgtga gcagtaacgc ataatcaatg agatacgtcc
     3601 cgagaacagg tatcggaaac aaccggacgc cgcatggctc tccaaaaaag cagcttgatc
     3661 cataccgcgg tggcttgcct tgtcattgcg gttccggcgc gtgccgatct taagccggaa
     3721 accaaccgcg cctttgaaca gtatgtcgcg aaagcggagc aaaggattgc cagacgcacg
     3781 aacggcggcg cccggtttct ctgggtggat gagtcgccgg cgcggatggc ggaagtccgt
     3841 cgcggtggcg tggcggccgg ctccaacatc gccaacaccg aagtggaggt tccggacggc
     3901 ctgatacacg actggaccgg
//
LOCUS       DASTMK010000275         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_460405, whole genome shotgun sequence.
ACCESSION   DASTMK010000275 DASTMK010000000
VERSION     DASTMK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460405"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFQ79_11980"
     CDS             <1..697
                     /locus_tag="VFQ79_11980"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0140430.1"
                     /translation="STVCFVGGNLAPEGAVIKATAIDPDVVDEDGVYRKTGPARVFTT
                     ERDAVAALKSGAIQAGDVLVLAGRGPMGSGMEETYQLTSALKHLPFGKHVALLTDARF
                     SGVSTGACIGHVSPEALAGGPLGKLRDGDRIEIAIDRNRLEGSADFTGDMRHPIAKEE
                     GARVLTSRPPNPGLAPDAALPPETRLWAALQDASGGTWGGAVFDVDRIIALLEAGKEA
                     SRNPTAPGQNISR"
     gene            complement(679..1785)
                     /locus_tag="VFQ79_11985"
     CDS             complement(679..1785)
                     /locus_tag="VFQ79_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0140431.1"
                     /translation="MASTAQFVTNDYSEKRELFSERVMQSVIGAFDVFTIYLGDRLGY
                     YQALKKQGALTSQELAASTGTSERYTCEWLEQQAAAAILEVEEPVEAPGMRKFRLPPG
                     HDEVLADHESLNYLAPMTQLFAGAVSPIRDLIAAFRTGDGIPFERYGPDLRDGQAGVN
                     RAMFLQQLGPEWLAAIPDLPRRLEADPPARVADIGCGVGWSSIGIARHYPKVLVDGFD
                     MDPSSIDLAQIYAKQYGVSGRVGFQCRDAGDSSLAGTYDLIVALECMHDMPNPVEALR
                     NMRRLVREDGFVLVVDERVSERFRPDAGEIERLMYGWSVLHCLPSGMTCDGVGTGTVM
                     RPATLQRYARAAGFKEIEILPVDHIFFRFYRLMF"
     gene            1886..>2733
                     /locus_tag="VFQ79_11990"
     CDS             1886..>2733
                     /locus_tag="VFQ79_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU0140432.1"
                     /translation="MIVGVPREIEDERRVALVPSVIPSLTKASFQVFVEAGAGVEAGY
                     PDAAFSDRGATIAADATELYKTAEVLLQVRSLRMDLLRPGQVVIGFLDPLGSPDKVNA
                     IARTGATAFSMELIPRITRAQSMDALSSMATVAGYKAVLLAADRLPKLFPMLMTAAGT
                     VTPAKVFVIGAGVAGLQAIATSRRLGAVVQAYDVRPAVRDQVESVGAKFVELPLETSD
                     TEGKGGYAKAQDEAFYTKQRELMGRVVADSDVVITTAAVPGKTAPVLVTKEMVAGMAS
                     GSVIVDL"
BASE COUNT          525 a          873 c          816 g          519 t
ORIGIN      
        1 ttccacggtc tgtttcgtcg gcggcaacct ggcccctgag ggcgctgtga ttaaagccac
       61 ggcgatagac ccggacgtcg tcgacgaaga cggcgtatat cgcaaaaccg gtccggcgcg
      121 tgtattcaca accgaacggg acgccgtcgc cgccctcaag tcgggcgcta ttcaagcagg
      181 agacgtactg gtgctcgcag gacgcggtcc gatgggctcc ggcatggagg agacctatca
      241 actcacctct gcactgaagc acctcccctt cggaaagcat gtggcgctgc tgacggatgc
      301 ccgcttcagc ggcgtttcga ccggggcttg catcggacac gtatctccgg aggcgttagc
      361 cggcgggccg ctggggaaat tgcgggatgg agatcgcatc gagatcgcca tcgatcgcaa
      421 ccgcctggaa ggctccgccg atttcacggg cgatatgcgc cacccgattg cgaaagaaga
      481 aggcgcgcgc gttctcacat cccgaccgcc caaccccggg cttgcgccgg atgccgcgct
      541 tcctcccgaa acccggctgt gggccgcgct tcaggatgcc agcggtggaa cctggggtgg
      601 cgcggtgttc gacgtggatc gcattatcgc tctactcgag gccgggaaag aagcctcccg
      661 gaatcccact gcgcccggtc agaacataag ccggtaaaac cggaagaata tatggtccac
      721 cggcaggatc tcaatctctt tgaaaccggc agcgcgagcg tagcgctgca acgtggccgg
      781 cctcataacg gttcccgttc ccactccatc gcacgtcatg cccgacggca gacagtgcag
      841 cacactccag ccgtacatca atcgctcgat ttcgcccgca tccggccgga accgctcgct
      901 cacacgctca tccaccacca gcacaaaacc atcctcccgg accagccggc gcatgttccg
      961 gagcgcctcg accggattgg gcatatcgtg catgcattcg agcgcgacga tcaggtcgta
     1021 agtccccgcc aggctcgaat caccggcatc ccggcactga aagccgaccc ggcctgagac
     1081 gccgtattgc ttcgcataga tctgcgccag gtcaatggaa gaggggtcca tgtcgaatcc
     1141 gtccaccagc actttcgggt agtgccgtgc gatgccgatg ctggaccacc ctacaccgca
     1201 gccgatatcc gccactcgcg ccggcggatc ggcctccagg cggcggggaa gatccgggat
     1261 cgcggcgagc cactccggac ccagttgctg caggaacatg gcacggttca cgcccgcctg
     1321 tccgtctcgc agatcgggcc cgtaacgctc gaacggaatg ccgtcgccag ttcgaaaggc
     1381 agctatcagg tctcgaatcg gactgacagc gccggcgaag agctgcgtca tgggcgcaag
     1441 ataattcagg ctctcatggt cagccagcac ctcatcgtgt cccgggggca gccggaattt
     1501 tcgcatcccg ggtgcctcga ccggctcttc gacttccaga atcgccgcgg cggcctgctg
     1561 ttccagccat tcgcaggtgt accgttccga ggttcccgtg ctggcggcga gttcctgcga
     1621 tgtcagggcg ccctgttttt tgagcgcctg gtagtacccg agccggtcac ccagatagat
     1681 ggtgaacacg tcgaacgctc ctattaccga ttgcatgacg cgttctgaaa acaattcccg
     1741 cttctcgctg taatcgttcg tgacgaattg agccgtggac gccaaagttt cctcctgaac
     1801 ctgcctgctc tcgcttaaga acgcgggaaa tcacacaaat ccggtcaagt ttttttggtc
     1861 cccggctttc ctttatagtc ttaagatgat cgttggcgtt ccaagggaga ttgaagacga
     1921 gcggcgtgtc gcgctggtgc cttccgtgat tccatcgctc acgaaggcat cttttcaggt
     1981 ttttgtggag gcgggagcag gtgtcgaagc cggctatccg gatgccgcct tctccgatcg
     2041 cggagcaact atcgccgcgg acgccaccga gctttacaag acggccgagg ttctgttgca
     2101 ggtgcgctcc ctgcgtatgg acctcctgcg gcccgggcag gttgtcatcg ggttcctgga
     2161 tccgcttggc tcacccgata aggtgaacgc aatcgcacgc acgggcgcca ccgcattcag
     2221 catggaactg ataccgcgca tcacgcgcgc acagagcatg gatgctcttt cctccatggc
     2281 aaccgtcgcc ggatacaagg ccgtcttact ggcagccgac cggttgccaa agctgtttcc
     2341 catgctgatg acagccgccg ggaccgtaac gcccgcgaag gtattcgtca tcggcgccgg
     2401 agtagcgggt ctacaggcca tcgcgacgtc ccgccgtctc ggcgccgtcg tgcaggctta
     2461 cgatgtacgt cccgcagtgc gcgaccaggt ggaaagtgta ggagccaagt tcgtcgagct
     2521 tccccttgaa acctcggata cggaaggcaa gggcggctat gccaaggcgc aggacgaggc
     2581 tttctacacc aagcagcgcg aattaatggg gcgcgttgtc gccgacagcg atgtcgtcat
     2641 taccaccgcg gcggtgcccg gcaaaacggc acccgttctc gtcacgaaag agatggtcgc
     2701 cggtatggct tccggatcgg tgattgtaga cct
//
LOCUS       DASTMK010000276         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_236961, whole genome shotgun sequence.
ACCESSION   DASTMK010000276 DASTMK010000000
VERSION     DASTMK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236961"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..2375
                     /gene="metE"
                     /locus_tag="VFQ79_11995"
     CDS             60..2375
                     /gene="metE"
                     /locus_tag="VFQ79_11995"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU0140433.1"
                     /translation="MATATNLGFPRIGANRELKKAVEAYWSGKSSGENLLAAASDIRR
                     SNWRLQADAGIEQIPTGDFSFYDHVLDTAFLFDLIPKRFRELKPASDLDLYFLMARGL
                     AGGQQTQALEMTKWFDTNYHYLVPEIGSDQHFTLARNTATDHFLEAAQLGYAARPVIL
                     GPVSFLWLSKSASDGSKPLHLLDRTRDAYEELLRRLASAGAAWVQMDEPVFAVDWNGR
                     ISPAALKQLYEHLAAAAPGVKILLSTYFGPLKENLEVALALPVHGLHLDLVRGPEQLE
                     HALRAAPSGLTLSLGLIDGRNVWRADLARALSVAEQAAAVVSADRIMVAPSCSLLHVP
                     VDLDAEDALEPEVREWLAFGKQKLQEVSLLAAALNKGRKAVETELKENAAVLARRNSS
                     PLVRNPAVRDRISSITAADRSHNSPYSIRKQAQASTLHLPAFPTTTIGSFPQTGQLRR
                     ERLRRKNGVIGEEDYKTFIRKEIEYAIRRQEEIGLDVLVHGEAERNDMVEYFGELLEG
                     FAFTKNGWVQSYGSRCVKPPIIYGDVLRRSAMTVDWWSFAQSLTQRPVKGMLTGPVTI
                     LQWSFVRDDQPRRDTCMQIALAVRDEVVGLEAAGCRVIQIDEPALREGLPLRAADAPA
                     YLSWAVDCFRIASAGVADSTQIHTHMCYADFNDIVDAIAAMDADVISLESARSQMVLL
                     EAFQKNRYPNELGPGVYDIHSPRIPGSSEMVNLLHRALAVIPPERLWVNPDCGLKTRQ
                     WDEVVPALQNMVAAARTLRSSAARDEVSSRR"
     gene            2372..2626
                     /locus_tag="VFQ79_12000"
     CDS             2372..2626
                     /locus_tag="VFQ79_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140434.1"
                     /translation="MRVLVLDNLDSFTWNLVHCFEAGGGRCKVVRSDAGTLADVRRFD
                     PERIVFSPGPFGPMQAGICKTVLQAYAGQVPILGVAWECN"
BASE COUNT          778 a         1096 c         1135 g          860 t
ORIGIN      
        1 cgcttcctgg tttttcctgc cttcgattct ccggaacaac tcgagaggag ctgaaaaaca
       61 tggcaaccgc cacgaatctg ggattcccgc gcatcggcgc aaatcgcgag ctaaaaaagg
      121 cagttgaagc gtattggagc ggcaagtctt ccggcgagaa cctgctggcc gccgcttcgg
      181 atatccgccg ctcgaattgg cggcttcagg cagacgccgg gatcgagcaa atccccaccg
      241 gagatttttc tttctacgat cacgtactcg ataccgcctt cttattcgat ctcattccga
      301 aacgctttcg ggaactgaag ccggcaagtg atttggacct ctacttcctc atggcccgcg
      361 gccttgcggg cggccagcag acccaggcac tggagatgac gaagtggttc gatacaaact
      421 atcactatct tgttccggag atcgggagcg atcaacactt cactctcgcc cggaacaccg
      481 ccacggatca tttcctcgaa gcggcacagc tcggctatgc ggcgagaccg gttattctgg
      541 gtcctgtttc cttcctttgg ttgagcaaaa gcgcaagtga cgggtcaaag cctttgcact
      601 tgctggatcg cacccgcgat gcgtatgagg aactgcttcg ccggctggca tccgccgggg
      661 ccgcctgggt gcagatggac gagccggttt ttgcagtcga ctggaacggc aggatctctc
      721 ctgccgctct gaaacagctt tatgaacatc tggcggcagc ggcgcctgga gtcaagatcc
      781 ttctctccac ttatttcgga cccttgaagg agaacctgga agtcgccctc gccttgccgg
      841 ttcacggact tcatcttgat cttgttcgcg gaccggagca actcgagcac gcgttgcgcg
      901 ccgcgccgtc aggcctcacg ctgtctctcg gacttatcga cgggaggaat gtctggagag
      961 ccgatctggc gcgtgcactc agcgtcgcgg aacaggcggc ggctgtggtt tcagcggatc
     1021 ggattatggt agccccgtcc tgttccctgc tgcacgttcc cgttgacctg gatgcggaag
     1081 acgcgctgga gcctgaagtc cgcgagtggc tggcctttgg taagcaaaag cttcaagagg
     1141 tgagcttgct cgccgcggcg ttgaacaaag gacgcaaggc agttgaaacc gaattgaagg
     1201 aaaacgccgc ggtcctggcg cgaaggaact cgtcgcctct tgtccgcaat cccgcggttc
     1261 gcgaccggat ctcgtcaatc acggctgccg accgttcaca caacagccct tattcgatcc
     1321 gcaagcaagc acaagcgagc actcttcacc tgcctgcctt cccaactacc accatcggat
     1381 cgtttcctca aaccgggcag ctgcgccgcg agagactgcg gaggaagaac ggcgtaatcg
     1441 gcgaagaaga ctacaaaaca ttcatcagga aagaaatcga atacgcaatc cgccgtcagg
     1501 aagagatcgg cctcgatgtt ctggtgcatg gcgaagcgga gcgcaacgac atggtggagt
     1561 actttggcga gcttttggag ggctttgctt ttacgaagaa cggctgggtt caaagctacg
     1621 gctcgcgatg cgtgaaacct ccgatcatct atggagacgt tttgcgaaga tccgccatga
     1681 ccgttgattg gtggagcttt gctcaatcct tgacgcaacg tccggtaaag ggaatgctca
     1741 cagggccggt cacgatcctc caatggtcgt tcgtgcggga cgatcaaccg cgccgcgata
     1801 cctgtatgca gatcgcgctg gccgtacgtg atgaagtggt gggtctcgaa gccgcgggtt
     1861 gcagggtcat tcaaatcgat gaaccggcgc tccgcgaagg gctgccgcta cgggccgccg
     1921 atgcccctgc atatttatcc tgggccgtgg attgtttcag gatcgccagc gccggtgtcg
     1981 ctgacagcac gcaaattcat acgcacatgt gttatgccga tttcaacgat attgtcgatg
     2041 cgattgcggc catggatgcg gacgtgatct cgctcgaaag cgcccggtcg cagatggtgc
     2101 tgctggaggc gttccaaaaa aaccgctatc ctaacgaact cggccctggc gtttatgaca
     2161 ttcactctcc caggattccc ggctcgtccg aaatggtgaa cctgctccat cgcgcgctcg
     2221 cggtcattcc accggagcgg ctttgggtga atcccgattg cggccttaag accaggcagt
     2281 gggacgaggt ggtaccggca ttgcaaaaca tggtagccgc cgcccgtacg ctccggtcgt
     2341 ccgccgcgcg ggatgaagtg tcatcgcgcc gatgagagtg ctggtgctcg ataaccttga
     2401 ttctttcact tggaatttgg tccactgctt tgaagctggc ggtggccgat gcaaggttgt
     2461 tcgttccgat gccggtacgc tcgccgatgt ccgccgtttc gaccccgagc gcatcgtctt
     2521 ttcaccgggt ccgtttgggc ctatgcaagc cggcatctgc aagacggtcc ttcaggctta
     2581 cgccggccag gttcccattc tgggtgttgc ctgggaatgc aactgatcgc gcatgcggaa
     2641 ggagcggacg ttgttccgtc aggaaacccg atgcacggca agacatccat ggtgcatcac
     2701 gatgaatccg ctcttttccg cggaattccc aatccgttcg aggcggcgcg ctatcactcg
     2761 ctgatcgtgc cgcgcgcttc gattcccccg catctgcaca tctccgcatg ggcggaggat
     2821 ggaacggtgc tcggttgtcg aatcccggat cgccgggtgg agggcatgct cgaatgcatc
     2881 gctccaaaac ggttaggccg tcctttctgg aatacagacc gtgatagggg ttttgcactt
     2941 gaatcttgtc catcctcctt ctgcgcagaa tcggacaatg agacctcttg acaacggaat
     3001 cttccaggtt cagaatacca gtagttaaac tataggaaga gatgagaccg cgacccgacg
     3061 aagtggttgg ggtgttgtcg gatggcgctc ggcgcgacga gaatcagcgt gttgaagttg
     3121 gtcgttcgac aggcagccgt atctgctgca ataggcacgg cgatcggttt tgttctcgcg
     3181 gtaaccgtgt cacgctatat ggcatcattg cttttctctg tccgtccgag cgatccgctg
     3241 acgttctttg cggtcgcact ggcactggcc gcaactgctg tgagcgcagt tcttgttccc
     3301 gctcgccggg ctacacacat agaccctatg gaggccctcc ggtatgaatg aactgtggcg
     3361 catttgcctt atctcttccg ccgtggtcgc tccaatcgcg accttgaaga tgagatgcag
     3421 ctccatctcg acatgaaggt gcgggaaaac cgggaggctg atccgggacc ggaagaggct
     3481 cgaatactaa ggcagggcga ccgaacccgc ggttgggcat ggtgaaacgt taccaagccc
     3541 gtggcgcgcc cacgtattgg ctggcaccac acacgtccag ccgccaagct ggggcgcaac
     3601 gggcgctccc ccctgcacca cgaggtggcg gtcctgtggc cgttgttggg agggtgctcc
     3661 tatctggcga ttttgaagtc ctgtctgagc aacataattt tactcaggca gtctgtaatc
     3721 actcaatgcc tacagaggcc acattgggac cttgatttcc gggaattttc gttttggacc
     3781 ctgcttggac catgatttgg tagtgggaag tctctagggc gtgttcacac taatagaaca
     3841 caatgatggt aaaatgtttg gaggatgga
//
LOCUS       DASTMK010000277         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_365466, whole genome shotgun sequence.
ACCESSION   DASTMK010000277 DASTMK010000000
VERSION     DASTMK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365466"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /gene="gndA"
                     /locus_tag="VFQ79_12005"
     CDS             <1..1223
                     /gene="gndA"
                     /locus_tag="VFQ79_12005"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR00873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU0140435.1"
                     /translation="ETPRKILIMVPAGKPVDSVIEDLKPLLSKDDILIDGGNSFFQDT
                     DRRGKDLESMGIRYIGTGVSGGEEGALHGPALMPGGQESAYKLVEPVLTAIAAKVNGD
                     ACSGYIGKGGAGHYVKMVHNGIEYGIMQLLCEVYDIMKSGLGMKAPEMHEVFTAWNEG
                     ELNSYLVEITAAVLAKTDPETNQPIVDVILDRAGQKGTGKWTSQNALDLGVAIPTINA
                     ALEGRILSAFKDERVAASKILAGPKVSFDGDRNALLGQLRNALRLSMLTCYAQGFALM
                     REASKEYVYHLDFTEIARIWRGGCIIRAKVLDDIRAVFAKNPEIANLVIAEPFSGIVN
                     ELAGDLRSVIGTATRLGVPCLAMCASLGYIDSYRNERLPANLLQAQRDYFGAHTYERV
                     DKPRGQKFHTEWLS"
     gene            1269..2576
                     /locus_tag="VFQ79_12010"
     CDS             1269..2576
                     /locus_tag="VFQ79_12010"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEU0140436.1"
                     /translation="MAANNNVVVAQSGGPSPVINNSLRGIIETCQMFPEKFGKIYGGW
                     HGIEGILREELLDLSSQSREEISLLRTTPAAGSIGTCRYKLKKNQQEDFERTVEVFKA
                     HNVGYFFYIGGNDSMDTAHKVANLAHERGLELAGVGVSKTIDNDVGDSEFKLIDHTPG
                     YGSVARYWAYNVQNANEENMGSSPADPVLVIQVMGRKIGFIPAAARLADPQREMPLQI
                     YMPESGVGLAELTDNVNDELKRSGRCIIVISEGFDAGDLGELKDAFGHTSFSSSIFTA
                     QQIVVNHLNKSGLKARGAARGQTAGTDQRDTMIYASTVDLDEAYKIGQNAVVIAMEHG
                     SGYMSTLLRRPGIIYSVDYDKVPLEQVANSERQFPKQWIAPNRIDVTDDFVRYARPLV
                     GEDWVSVPVVNGVQRYARIQKIFAEKKLPEYILQGHRPKAAAQ"
     gene            2594..3481
                     /locus_tag="VFQ79_12015"
     CDS             2594..3481
                     /locus_tag="VFQ79_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140437.1"
                     /translation="MSDPQAQLKNFQKKHDFLIAIDSDGCAFDTMEIKHKECFIPNIV
                     KYWNLQPVSKYARAAAEFVNLYSKWRGINRFPALTMVFDLLKDWPDVQKRHASIPEAQ
                     PVRDWIKRETKLGNPALKAEVGKNGDPVMKQALEWSEAVNRTIADIVHDVPPFPFVRE
                     SLEKVSEWADVLVCSATPGEALVREWQEHGIHVFVGCIAGQEMGSKKEHIALANASRY
                     DAGKILMIGDAPGDFKAAQGNNARFFPINPGHEDDSWELFFKEAAEKFRDGAYTPEYE
                     GKLIAEFDKLLPELPPWKK"
     gene            3469..4065
                     /locus_tag="VFQ79_12020"
     CDS             3469..4065
                     /locus_tag="VFQ79_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU0140438.1"
                     /translation="MEKITAGNRNEEAVPIGGLPASLIEKASKIRLLLMDVDGVLTDG
                     KLYNVPDAGGNMVETKAFDSQDGIALQWLGWHGIKTGLISGRISPATVERARQCNFSY
                     VYQGHIEKIPILEEIAADCNLSRDEMAYIGDDFTDVVVMRRVSLAIATANARPEVKRS
                     AHYVTTQPGGRGAVREVVELLLTAQGHWPGLLKRYEID"
     gene            4065..>4487
                     /locus_tag="VFQ79_12025"
     CDS             4065..>4487
                     /locus_tag="VFQ79_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HEU0140439.1"
                     /translation="MPSERIGLMIRADSGTGILHQLTGVIARHTGDITSVEIIESRLP
                     ESQVYFEVDLPGESTRLRDELASLAIVRDVQIVNSLQKIYGKRIIIMGGGAQVGQVAI
                     GAISEADRHNIRGERISVDTIPLVGEQPLADAVRAVARL"
BASE COUNT         1004 a         1232 c         1384 g          867 t
ORIGIN      
        1 tcgagactcc gcggaagatc ctcatcatgg ttccggccgg aaagccggtc gactccgtca
       61 tcgaagacct caagccgctg ttgagcaaag acgacattct gattgatggc ggcaactcgt
      121 ttttccagga cacggaccgg cgtggcaagg atctggaatc gatgggcatt cgctacatcg
      181 gaacgggcgt gagcggcggg gaagaaggag cattgcacgg ccccgcgctg atgccgggcg
      241 gacaggaatc cgcttataag ctggtggagc cggtcttgac ggccattgcg gcgaaggtga
      301 atggggatgc gtgctccggc tatatcggta aaggcggggc ggggcattac gtcaagatgg
      361 tgcacaacgg catcgagtac ggcatcatgc aactgctctg cgaggtttac gacatcatga
      421 agtcgggcct cggcatgaaa gcgccggaga tgcacgaagt ctttaccgcg tggaacgagg
      481 gcgaacttaa ctcctacctg gttgagatca ctgctgccgt gctggccaaa accgacccgg
      541 agacgaatca gccgattgta gacgttatcc tggaccgtgc gggacaaaag ggaaccggaa
      601 aatggacctc gcagaacgcg ctcgacctcg gcgtggcgat cccgaccatc aacgcggcgc
      661 tcgaaggacg gattctttcc gctttcaagg acgaacgtgt ggcggcatca aagatcctgg
      721 ctggcccgaa ggtttctttc gacggcgaca ggaacgcgct gttggggcag cttcggaacg
      781 cgctgcggct ttccatgctt acctgttacg cgcaaggatt cgccctgatg cgcgaagctt
      841 ccaaagagta cgtttatcac ctggatttta cggagatcgc gcgcatctgg agagggggtt
      901 gcatcattcg cgccaaggtg ctcgacgaca ttcgcgctgt ctttgcgaag aaccccgaga
      961 ttgcaaacct cgtgatagcg gaacccttca gcggcattgt gaacgagctc gccggcgatc
     1021 ttcgatccgt aatcggcaca gcgacgcggc ttggcgtgcc gtgtcttgcg atgtgcgctt
     1081 cccttggcta tatcgatagt taccggaacg aacggctccc cgccaatctt cttcaggcac
     1141 agcgggatta cttcggggcg catacctatg agcgcgtcga taagccgcgc ggccagaagt
     1201 tccataccga gtggttatct taatctcggc tccggcaatg cggccggcgc caggagtgta
     1261 ttcataaaat ggcggcaaat aacaatgtag tggttgcgca gtcgggaggg ccctcgcccg
     1321 tcatcaataa ctcactgcgt ggaatcatcg aaacctgcca gatgttcccg gagaaattcg
     1381 ggaaaatcta cggtggctgg catggcatcg aaggaatact gagagaggag ctgctcgatc
     1441 tctcctcgca gtcccgcgaa gagatcagcc tgttgcgaac gactccggcg gcaggctcca
     1501 tcggaacctg ccggtacaag ctgaagaaga accagcagga ggatttcgag cgcaccgtcg
     1561 aggtcttcaa ggcacacaac gtcggctact tcttttatat cggcggcaac gattccatgg
     1621 acaccgccca caaggtggcg aatctcgcgc acgaacgggg cctggagctt gcgggcgtcg
     1681 gtgtgtcgaa gaccatcgac aacgacgtcg gcgattccga gttcaagctg atcgatcaca
     1741 cgcccggcta tggcagcgtt gcacgctact gggcctacaa cgtgcaaaac gccaacgaag
     1801 agaacatggg ctcgtcgccg gcggatcccg tcctggtgat acaggtgatg gggcgtaaga
     1861 tcggcttcat tcccgccgcg gcccggctgg ccgatccgca acgcgaaatg ccgcttcaga
     1921 tttacatgcc ggaatcgggc gtcggcctgg cggaattgac cgataacgtg aacgacgagt
     1981 taaagcggag cggccgctgc atcatcgtga tcagtgaagg tttcgatgcc ggtgatctcg
     2041 gagagttgaa ggatgccttc gggcatacga gcttcagctc cagcatcttc accgcacagc
     2101 agattgtagt gaaccatctg aataaatccg gcttgaaggc caggggagcg gcgcgcggcc
     2161 aaacggccgg gaccgaccag cgcgacacga tgatctacgc ttccacggtg gatttggacg
     2221 aggcttacaa aatcggccag aacgcggtcg tgatcgcgat ggaacatggt tccggatata
     2281 tgtccacgct gctgcgccgg ccgggcatta tctacagcgt cgattacgat aaggttcctc
     2341 tcgagcaggt ggccaactcc gagcgccagt tcccgaaaca gtggattgcg ccgaaccgca
     2401 tcgacgtgac ggacgacttc gtgcgttacg cgcgcccgct ggttggagaa gactgggtga
     2461 gcgttccggt cgtgaacggc gttcaacggt acgcgagaat tcagaagatt tttgccgaaa
     2521 agaaattgcc cgagtacatc cttcaagggc accggcccaa ggcggcggct caataagcat
     2581 caggagacag ttgatgtcag atcctcaagc acagttgaag aatttccaga aaaaacacga
     2641 tttccttatt gccatcgatt cggatggctg cgcgtttgac acgatggaga tcaagcacaa
     2701 agagtgcttc atccccaata tcgtcaaata ctggaatttg cagcccgttt ccaagtatgc
     2761 gcgcgccgcg gccgagttcg tgaatcttta ttcgaagtgg agaggcatca atcgctttcc
     2821 cgcgctgacc atggtattcg acctgttgaa ggactggccg gacgtgcaga aacgccacgc
     2881 ctcgattccc gaagctcagc ccgtgcggga ctggatcaaa cgggaaacga aactcggcaa
     2941 tccggcgctg aaggccgaag tcgggaaaaa cggcgatccg gtgatgaagc aggcgcttga
     3001 atggagcgaa gcggtgaacc gtacgatcgc cgacattgtt cacgatgtgc cgccatttcc
     3061 attcgtgcgg gaaagtctgg agaaggtctc ggaatgggcc gacgtgctgg tatgttcggc
     3121 gactccgggc gaggcccttg tacgcgagtg gcaggagcac ggtatccacg tgttcgtggg
     3181 ctgtattgca ggccaggaga tgggcagcaa gaaagagcac atcgcgctcg cgaacgccag
     3241 ccggtacgat gccggcaaga ttctcatgat tggggacgca ccgggcgatt tcaaagcggc
     3301 acaggggaat aacgcgcggt tctttcccat caatcccgga cacgaagacg actcctggga
     3361 actctttttc aaggaagcgg ctgaaaagtt tcgcgacggc gcctacacgc ccgaatatga
     3421 aggcaaactc atcgccgagt tcgacaagct ccttccggag cttcctccat ggaaaaaata
     3481 acagcaggta accggaacga ggaggctgtc ccgatcggcg gcctccccgc ttctctcatc
     3541 gaaaaggcct cgaagatacg cctgcttctg atggacgtcg acggcgtgct gacggatggc
     3601 aagctgtaca acgttccgga tgccggcggg aatatggtgg agaccaaggc gttcgactcg
     3661 caggatggga ttgcgctcca atggttgggc tggcatggta tcaagacggg cctcatcagt
     3721 ggccggattt cgccggcgac cgtcgagcgg gcacggcagt gcaacttctc ttacgtctat
     3781 cagggccata ttgaaaagat ccctattctc gaggagatcg cggcggactg caatttgagc
     3841 cgggatgaaa tggcatatat cggcgacgat ttcacggatg tcgtagtaat gcggcgcgtg
     3901 agcctggcca tcgcaaccgc caatgccaga ccggaggtga agcgctccgc tcactacgta
     3961 actacgcagc ccggcggccg gggggcggtg cgtgaggttg tcgagttgct ccttacagcc
     4021 caggggcact ggcccgggct gctgaaaaga tacgaaatcg actgatgcct tcagagcgaa
     4081 tcggactgat gatccgggcc gactccggca ccggtatcct gcaccagttg accggtgtga
     4141 tcgcgcgcca taccggtgac atcacttccg tcgagatcat cgaatcgcgc ctgccggaat
     4201 cgcaggtcta tttcgaagtc gacctgcccg gcgaatccac gcgtctgcgg gatgagcttg
     4261 cgtcgctggc catcgtgcgg gatgtgcaaa tcgtcaacag cctgcaaaag atttacggaa
     4321 agcgcattat catcatgggc ggaggcgccc aggtgggcca ggttgctatc ggcgctattt
     4381 cggaggccga tcgtcacaac atccgaggcg agcgcatatc cgtggacacg attccactgg
     4441 taggcgaaca acccctggcg gatgcggtgc gcgctgtagc gcgcctg
//
LOCUS       DASTMK010000278         8345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_526079, whole genome shotgun sequence.
ACCESSION   DASTMK010000278 DASTMK010000000
VERSION     DASTMK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526079"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFQ79_12030"
     CDS             complement(<1..742)
                     /locus_tag="VFQ79_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140440.1"
                     /translation="MTCRYVRRHTADLFDPGAAPEFTAELRRHLAACPTCAAELEAMT
                     QMLASLKPPPIRASADFKERTMNKLAARLAALQAADTNKPHLNSRVPRLFWRFAFIAA
                     AIVALIVGLPYLGRPGGSQSAAMTLLAQSVQAASNLQSVHLIARMRTLPGDNFELIGT
                     QYDFVPLEMWKEFGNPPRWRVEKPGRVVVMDGASSLLLFRRNQAVRGGPNTGFVDWLK
                     PLLDADKVLEKELAAAQKGESQVSLAEQT"
     gene            complement(739..1338)
                     /locus_tag="VFQ79_12035"
     CDS             complement(739..1338)
                     /locus_tag="VFQ79_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0140441.1"
                     /translation="MEWTQSFTLDDASANPDTLPAAAEAAEHWPQSITEFEALVDAHQ
                     DELLNFAFCRLHNLADAEDIVQDVLVQAYVEREKHKKIARVRPYLYRMVSNRCTDVLR
                     KRRRSWRLLNRVGAQDLPAAQDPEPAPVEGSLRRRQIEELFSKLPSRQAEVMRLRVFA
                     GLPFRAIAEAVGASVPTIKSRFRYGAERLRSILSKEAER"
     gene            1444..3120
                     /locus_tag="VFQ79_12040"
     CDS             1444..3120
                     /locus_tag="VFQ79_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0140442.1"
                     /translation="MTRLLSILLGSAACISAQSSFQVVEATIPEMQAALREGHVTSQE
                     LVRSYLVRIALYEDKLNAIITVNPRAIEEAERLDKERAQGKVRGPLHGIPVALKDNIH
                     TTDMPTTGGALAFAELVPPYEATLTKNLREAGAIIIAKTGLTELANWVASGMPGNYNA
                     VAGYGFNPYDPRRDPRKGIADGRPALSTSGSSSGIGTAANFWAANVGTETSGSILSPS
                     NQNMLVGIKPTVGRVSRHGIIPITADQDTAGPMARTVVDAAILLGALENTSPDPNDRA
                     TARCAPPPGRDYTRFLNRNGLKGARIGVPRAYFIDAVNAPGAKSPRGGLTPDQKKVVE
                     EAIVVLRREGAVVVDPADLESVTSADPQRNLMNWDTCAGPNGAKGRDENCSIVFKYGM
                     KRDFNKWLASLGSAAPVKTLTELRLFNLAHREAGAIKYGQAQLDISDEMDVEADRARY
                     EADRAKDLALAATHGIDAAMTQHRLDALLFAWGSGASVAAKPGYPTIIVPFGWVPNAG
                     RVPFPEGFNPKPMPFGVSFTGMACSESRLIEIAYAFEQATKRRVPPPSVP"
     gene            complement(3144..3701)
                     /locus_tag="VFQ79_12045"
     CDS             complement(3144..3701)
                     /locus_tag="VFQ79_12045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEU0140443.1"
                     /translation="MSSYQIVVVVGSLRRQSFNRQLADAVIKLGPPDFSFQHARIDDL
                     PLYNQDDDANPAEPVKRLKGEIAAAHGLLFVTPEYNRSIPGVLKNAIDHASRPYGQNA
                     WAGKPAGVLGASIGTTGTAMAQQHLRNILAYLDVPVLGQPEAFIRVKDGLFDKDGNIT
                     GDGTRKTLQGWMDRYVAWVKKVAAR"
     gene            3951..5000
                     /locus_tag="VFQ79_12050"
     CDS             3951..5000
                     /locus_tag="VFQ79_12050"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU0140444.1"
                     /translation="MQRNDGLLQVRPANILPDRDARIVRTTGIVQALEWQSIRVPQMT
                     GEGGSNITLTAIVPNGAKVSKGDVLVEFDRLNLLDQERDAIGLLDNLGHQLEERRAQV
                     NSLQTTRGSQIRQAQADLERAQLQLRKGPILGDMDRMKNEAKAEDARLRLDSLKRSDA
                     LRAKSETASIRILELKNERQRVTLERIRTNLDRLVIKAPQDGMVAHENTWRQGSMGPP
                     QVGDRMWPGMPVLRIFNPARMVVQATVDEPDFASISNAAHARVYLDAYPEESFDATLQ
                     SASPVATAGLDSPVRNFIAIFLLEQQSPRLLPDLSAALEVERRSTATPVSTGTTSTEP
                     VAKAGGREPMARANR"
     gene            4997..6313
                     /locus_tag="VFQ79_12055"
     CDS             4997..6313
                     /locus_tag="VFQ79_12055"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU0140445.1"
                     /translation="MSVAVAPAQLSNAPPASPSPRRRRRMVIAWLFALLALAGSAVLA
                     VAVFRPAAAQPSTTLPLATARRGDFLVTVTCRGELVAEDSVQIIAPNVPNLTIVWMAP
                     PNSAVKEGDPVVRFDASGAQRQLKEKEAALAQAVASADQAKAEAGITVEQDRLEIASL
                     EQAVELAKIEVSKSQILSAIQAEDNRITLALAEEKLRVKQAAAVFNAASAQSKIASAE
                     SQRKKADAEVQLIRERIARTEIRAPGSGVINYLMNYSQGWMNAKPFKVGDNVWPGSAV
                     AEIPNLESLRLKGKTEEIDRGRMSAGQDVRVILDPFPEKTFAGKLDRISPLTEQSWEW
                     PPTRSFRAYATLGETDNRLRPQMNGRMDITVDRIPNAISVPSKAVFARDGKPVVLVPG
                     KDGVRAVPIELLARNPDEVAIRGIEAGTQVALVDNPSDERKKGESK"
     gene            6310..7608
                     /locus_tag="VFQ79_12060"
     CDS             6310..7608
                     /locus_tag="VFQ79_12060"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140446.1"
                     /translation="MNRLPRVIFVLAVLIVLAAGARSVPRLFEKINKPDPTPLPSTRV
                     RTGDVTFTVTADGALQGGNSRMLTAPMAGGGTLTLTELRKSGELVKKNDVVAEFDTTD
                     ETYKLRQAEADLAEAEQQVIQAGNEAKAKQEEIEYELITARGELKQAEIEVMANPIRA
                     ALTAKQNDITLEGARNKLAKLERDYPQRKAAASASIAIQEAARKKAQVLADTARRNIE
                     MMTLRAPADGYVNVERNTNTNFYFSGMVLPLFQVGDQVRPGMAVAQIPDLDSWDVAAQ
                     IAETDRGHLSVGLSADIRVIALPGHKFAAKVKDLGGTTGPPWQRRFECKLALLQAVPE
                     LRPGMSARIVVTLETLKNVMWLPAQALFEQGGRTFVYARHGERFLAKDVKLVRRGESQ
                     VVITGLQPDEEVALASPDQKNQKDQKKQAASPASAGGVSK"
     gene            7605..>8345
                     /locus_tag="VFQ79_12065"
     CDS             7605..>8345
                     /locus_tag="VFQ79_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0140447.1"
                     /translation="MTPALETEPAPPQPAGVVLQRRVVGIFKFVTDFGEALGNLRAQK
                     TRTLLTALGIVFGVGSVIGMLSIGAGAREQSLQFIERLGVRNILVDSRPVSTREELQQ
                     RRRVSPGLTERDVRILEANVDALETLSPRRFLHPARVLPKPLGTMPDMFGVRASYQII
                     HDLRPIEGRFLSTEDEETSASVCVLGLNAKIGMLGYEPAVGKHVKVNDTWLRVVGVLA
                     EQVADSGSGRAVDRNNVIFIPISTFRYRF"
BASE COUNT         1731 a         2498 c         2608 g         1508 t
ORIGIN      
        1 ttgtctgctc cgcgagagat acctgcgatt cgcccttttg cgcggccgcc agttccttct
       61 cgaggacttt atcggcgtcc agaaggggtt tcagccagtc gacgaaaccg gtattaggac
      121 ctccgcgaac ggcctgattc ctcctgaaca gcagcaggga ggatgcgccg tccatcacca
      181 ccacgcgtcc cggtttttcc acgcgccatc gtggcggatt gccgaactcc ttccacattt
      241 cgagcggaac gaagtcatac tgcgttccga tcaactcgaa gttatcgccg ggcagcgtgc
      301 gcatccgggc aatcagatgg accgattgaa ggttcgatgc ggcctgcacc gactgagcca
      361 gcaaggtcat ggcggcggat tgactccctc cgggccggcc gagatatggc agtcctacaa
      421 tgagagccac tatggcggcg gcgatgaagg cgaaccgcca gaataaacgt ggaacacgcg
      481 aattgaggtg tggcttgttt gtatcggccg cctgaagggc ggcgagacga gctgcgagtt
      541 tgttcatggt gcgctccttg aaatcggcgg aggcgcgaat cggcggcggt ttcaacgaag
      601 cgagcatctg agtcatcgcc tctaattcag cggcacacgt ggggcatgct gccaggtgcc
      661 ggcggagttc cgcggtaaat tcgggagcgg cgccggggtc gaaaagatcc gcagtgtgcc
      721 ggcgaacgta tcggcacgtc atcgttcagc ctccttggac agaatgctgc ggagccgttc
      781 cgctccgtag cgaaagcgcg acttgatagt gggtaccgaa gcacccacgg cttctgcgat
      841 agctcgaaaa ggcagtccag cgaaaacacg cagccgcatg acttcggcct ggcgcgaagg
      901 cagcttggaa aacagttcct cgatttgacg ccggcgcaag ctgccttcaa ccggtgcggg
      961 ttccggatcc tgggctgcgg gaagatcctg cgcgccaacc cggttcagca accgccaact
     1021 tcgtctgcgt ttgcgcaaca catccgtgca gcggttcgag accatacggt acaggtaggg
     1081 gcgaaccctg gcaattttct tatgcttttc acgttccacg taagcttgta ccagcacgtc
     1141 ctgaacgatg tcttccgcgt ccgccagatt gtgaagccgg caaaacgcga agttcagaag
     1201 ttcgtcctgg tgggcgtcca ccagtgcctc aaattccgtt atggattgag gccagtgttc
     1261 ggctgcctcg gcagccgccg ggagcgtatc agggttagcg gatgcgtcat ccaaagtgaa
     1321 cgattgcgtc cattccatca cagtgagcct gtctgccggt ataacgaacg gcgtgacgaa
     1381 aagatttaaa cgaagccgga gtgaacggtg ccggacaagt gtaatatagg aaactcaatc
     1441 gccatgacca gactgctttc catcctgctt gggtcagccg cttgtatatc tgcacaatcg
     1501 tcgtttcagg tggtggaagc cacgattccc gagatgcagg ccgccctgcg cgaaggccac
     1561 gtcacatcgc aggagcttgt ccggagctac ctggtacgga tcgcgctcta tgaagacaag
     1621 ttgaatgcga ttatcaccgt aaacccgcgt gccatcgagg aagcggagcg gcttgacaag
     1681 gaacgggcgc agggtaaggt gcgtggccct cttcacggaa tcccggttgc gttgaaagac
     1741 aatattcaca ctacggacat gccgaccacc gggggagcgc tggctttcgc ggagctggtg
     1801 ccgccctacg aagcgaccct tacgaaaaat ctgcgggagg ccggcgcaat catcatcgcg
     1861 aagaccgggc ttacggagct tgccaactgg gtagccagcg gtatgccggg caactataac
     1921 gcggtcgccg ggtacggttt caacccatac gatccgcggc gcgaccctcg caaaggcatt
     1981 gccgacgggc gcccggcgct atcgacaagc ggatcgagct ccggcatcgg aacggcggcg
     2041 aacttctggg ccgcgaacgt gggcaccgag acttcgggtt ccatcctgag cccttccaat
     2101 cagaacatgc tggtgggtat aaagccgacc gtcgggcgtg tgagccggca tggcatcatc
     2161 ccgatcaccg ccgatcagga caccgccggt ccgatggccc gcacggttgt ggacgcggcg
     2221 attctgctgg gtgcgctcga aaacacgtcg cccgatccga acgatagggc tacggcgcgg
     2281 tgcgctccac cgccggggcg cgactacacg cgcttcctga accgcaatgg gctgaaaggc
     2341 gcccgcatcg gagttccccg cgcgtacttt atcgacgccg taaacgcccc gggcgccaag
     2401 tcgccgcgcg gcgggttaac gccggaccag aaaaaagttg tggaggaagc gatcgtggtt
     2461 ctcaggcggg agggggcggt cgtcgtcgac ccggccgatc tggagagcgt gaccagcgcg
     2521 gatccgcagc gcaaccttat gaactgggac acctgtgcgg gccccaacgg cgcgaagggg
     2581 agggatgaga actgctcgat tgtattcaag tacggcatga agcgggattt caacaagtgg
     2641 ctggcgtcgc tcggctccgc cgcgccggtg aaaacgctta cggaactgag gctgttcaac
     2701 ctggcacatc gcgaggcagg ggcgatcaag tacggccagg cgcaactcga catctcggac
     2761 gagatggatg tggaggcgga ccgcgcacgt tacgaagcgg accgcgcgaa ggatcttgcc
     2821 cttgccgcga cgcacgggat tgacgcggcg atgacacaac accgccttga cgccctgctg
     2881 tttgcctggg gcagtggggc atcggtggca gcgaaacccg gttatcccac aatcatcgtt
     2941 ccgtttggct gggtgccaaa tgcgggccgg gtgccgtttc cggaaggatt caacccaaag
     3001 ccgatgccgt tcggggtgag ctttaccggt atggcttgca gcgagtcgcg cctcatcgaa
     3061 atcgcctacg cctttgaaca ggcgacgaag cgtcgcgttc cgccgccctc ggtaccctga
     3121 gcgcaaatcg ccctcgccgt ctgttatcgc gccgcgacct ttttcaccca ggcgacatag
     3181 cggtccatcc agccctgcag ggtcttccgg gtgccgtcgc cggtgatatt accgtccttg
     3241 tcaaagagcc cgtccttaac acggatgaag gcttcgggtt ggcccagcac cggcacgtcc
     3301 aggtacgcga gaatgttgcg caaatgctgc tgcgccatag cggtgccggt tgtaccgatc
     3361 gatgcgccca gcacgccggc gggcttgccc gcccaggcgt tctgcccgta aggacgggag
     3421 gcgtgatcga tggcgttctt gagcactccg ggaatagaac ggttgtactc cggggtgacg
     3481 aacaaaaggc catgggcggc cgcgatctca cccttcagcc gcttgaccgg ctcggctggg
     3541 tttgcatcgt cgtcctgatt gtaaagcggc aagtcgtcga ttcgcgcgtg ctggaacgag
     3601 aaatccggcg gccccagttt gatgacggcg tctgccagtt ggcggttgaa tgactgcctg
     3661 cgaagactgc cgaccactac aacgatttga tatgaactca tgttgatctc tgaagtttac
     3721 actaccgcca gccgagctcg aaaacagact catgggtccc gaatcagaaa ctgaaaaaaa
     3781 agtaccacat tgcgtaaaca aaattcagga ttcattcgtc taaaattgct gaagacaatc
     3841 ttgtgactac caagcgaagg ctccacgcgg gtgcgctggt acttgtgacg gctgtgatct
     3901 tcgccgggtg ttcccctcct gattcttcca aacagcaaca atcggacccg atgcaacgga
     3961 atgatggttt gttgcaggtg cggcctgcaa acatcttgcc ggatcgcgat gcgcggattg
     4021 tccggacaac cggcattgtg caggcgctcg aatggcaatc tatccgtgta ccccagatga
     4081 ccggagaggg cgggtccaat atcaccctca cggccattgt tccgaacgga gcaaaggtct
     4141 cgaagggcga tgtgctcgtc gagttcgatc gcctgaatct cctcgaccag gaacgcgacg
     4201 caatcgggct gctggacaat ctcggtcacc agttggaaga acggagagct caggtcaaca
     4261 gcttgcaaac cacccgcgga tcgcaaatcc gccaggcgca ggccgacctc gagagagcgc
     4321 agttacagtt gcgcaaaggg ccgatattag gcgatatgga tcggatgaag aacgaggcca
     4381 aagccgaaga cgcgcgattg cgcctggaca gcctcaagcg atccgatgcc ctgcgcgcca
     4441 agagcgaaac ggcttcgatt cgaatcctcg aactaaagaa cgagcgccag cgtgtcacgc
     4501 tggagcggat ccgcacgaac ctggaccgcc tcgttattaa agcgccgcag gatggtatgg
     4561 tcgctcatga gaatacctgg aggcaaggct ctatgggtcc gccgcaggtc ggcgatcgca
     4621 tgtggcctgg tatgccggtt ctgcgcatct tcaacccggc ccgcatggtc gtgcaggcca
     4681 cggtcgacga gcccgatttc gcatcgattt cgaatgcggc tcacgcgcgg gtctacctcg
     4741 acgcttaccc ggaagagtcc ttcgatgcga cactccaatc cgccagcccg gttgcgacag
     4801 cgggcctgga ctctcccgtg cggaatttca tcgcgatttt cctactcgaa caacagagcc
     4861 cgcgattgct gcctgatctc tcagccgcct tggaggtgga acggcgctcg accgctacac
     4921 cggtttcaac cggaaccacc agtaccgaac cggtagcgaa ggcaggtgga cgcgaaccga
     4981 tggcgagggc gaaccggtga gcgtcgctgt cgccccggcc caactctcga acgcccctcc
     5041 ggcgagcccc tccccacgcc ggcgccgccg tatggtgatc gcctggttat ttgcattact
     5101 cgccctggct ggttccgccg tccttgccgt cgccgttttc cgtccggcgg cggcgcaacc
     5161 ctccacgacc ctgcctctgg ccactgcccg gcgcggcgac tttctcgtga cggtcacctg
     5221 ccggggcgag cttgtggcgg aagattcggt gcagattatc gcgcccaatg ttccgaacct
     5281 gaccatcgtc tggatggcgc cgccgaattc cgccgtcaaa gaaggcgatc cggtcgtccg
     5341 cttcgacgcg agcggggcgc agagacagtt gaaggagaag gaagcggcat tggcgcaggc
     5401 agtcgcatcg gccgatcagg ccaaagcgga agcgggcatc acggtggagc aggaccggct
     5461 cgagattgcg agcctggagc aggctgtcga attagccaag attgaagtgt cgaagtccca
     5521 gatcctgagc gccatccagg cggaggataa tcgaatcacg ctcgcgctgg ccgaagagaa
     5581 actgcgggtc aagcaggctg ccgccgtatt caatgcggcg tccgcccagt cgaaaatcgc
     5641 atccgccgaa agccagcgca agaaagcgga tgcggaggtg caactgatac gcgaacgcat
     5701 cgcgcggacc gagattcgcg cacccggcag cggcgtgatc aactatctca tgaactattc
     5761 gcagggctgg atgaacgcca agccttttaa agtgggcgac aacgtctggc cgggcagcgc
     5821 cgtagccgag attcccaatc tcgaaagcct gcgattgaag gggaagaccg aggagatcga
     5881 tcggggccgg atgagtgccg ggcaggacgt ccgtgtgatt ctcgatccct ttccggagaa
     5941 aacgtttgca gggaagctcg atcgcatctc gccgctgacg gaacaaagct gggaatggcc
     6001 gccgacgcgc agtttccgtg cctacgcgac actcggggaa accgacaacc gtctgcgtcc
     6061 acaaatgaac ggacgcatgg acatcaccgt ggacagaatc ccgaacgcca tcagtgtgcc
     6121 gtcaaaagct gtgttcgcgc gggacggaaa gcccgtggtg ctggtgccgg gtaaggacgg
     6181 cgtgcgggct gtcccgatcg agttgctggc ccgtaacccg gacgaggtcg caatccgggg
     6241 cattgaagcg ggaacgcagg ttgccctggt cgataacccg agtgacgaga ggaagaaagg
     6301 cgagtccaaa tgaatcgtct tccacgcgtg atattcgtgc tcgccgtcct catcgtcctg
     6361 gccgccggtg cccgctccgt accgcgcctg ttcgagaaaa tcaacaagcc ggaccctaca
     6421 ccgctgccct ccacgcgcgt caggacgggc gacgtcacgt tcaccgtcac cgcggatggc
     6481 gccttacagg gcggtaattc gcgcatgctc accgcgccca tggccggtgg cggcaccttg
     6541 actctgacgg agctccgcaa gtccggagag ttggtgaaga agaacgatgt ggtcgctgaa
     6601 ttcgacacta ccgacgaaac ctataagctt cgccaggcgg aggcggacct cgcggaagcc
     6661 gagcagcagg tgatccaggc cgggaacgag gcaaaggcca agcaagaaga gatcgagtac
     6721 gaattaatca cagcgcgcgg cgagctgaag caggccgaaa tcgaagtgat ggcgaatccc
     6781 attcgggccg ccctgacagc gaagcagaat gacattactc tcgagggagc caggaacaaa
     6841 ctcgccaagc tggaacgcga ttatcctcag cgcaaagctg cggcgagcgc ttccatagcc
     6901 attcaggaag cggcgcgtaa gaaggcgcag gtgctcgccg atacagcgcg ccgcaacatc
     6961 gagatgatga ccctccgggc tcccgccgac ggctatgtca atgtcgaacg gaatacgaac
     7021 acgaatttct atttctcggg catggtcctg ccgctgttcc aggtcggcga ccaggtgcgg
     7081 cccggcatgg cggtagccca aattcccgac ctcgatagtt gggatgtagc ggctcagatt
     7141 gccgagacgg accgcggaca tctctccgtc ggcctatcgg cggacattcg tgtgatcgcg
     7201 ctgccgggcc acaagtttgc ggcgaaggtg aaggaccttg gtggaacgac cggtcccccc
     7261 tggcagcggc ggtttgagtg caagctcgcg ctgctgcagg cggtcccgga acttcggccg
     7321 gggatgagcg cgcgtatcgt ggtcacactc gaaacgctaa agaacgtcat gtggcttcct
     7381 gcccaggcgc tgttcgaaca aggcggccgg acgttcgtct acgcgcgcca tggagagcgg
     7441 tttctggcca aagacgtgaa actggtgcga aggggtgaaa gccaggtcgt gatcaccggc
     7501 ttgcaaccgg acgaggaggt cgcgctcgcg agccctgacc agaaaaatca gaaagaccag
     7561 aagaaacagg ctgcctctcc ggcctcggcg ggaggcgtct cgaaatgacg ccggcactcg
     7621 aaacggaacc cgctccgccg cagccggccg gtgtggttct ccagcggcgc gtggtgggga
     7681 ttttcaagtt cgttacggat ttcggcgaag cgctcggaaa cctgcgcgcc cagaaaacgc
     7741 ggacactcct gacggctctc ggtatcgtct tcggtgtcgg ctccgtcatc ggtatgttgt
     7801 ccataggtgc gggcgcccgg gagcagtccc tacagttcat tgagcggctc ggtgtccgga
     7861 acattctggt ggattcacgg cccgtctcta cgcgcgagga actccagcag cgccgccgcg
     7921 tttcacctgg tctcaccgaa cgcgatgtgc gcatcctcga ggcgaatgtc gacgcgctcg
     7981 agaccctgtc accccggcga ttcctgcacc ccgcgcgcgt gctgcccaag ccgctgggga
     8041 cgatgccgga catgttcggt gtccgtgcgt cgtaccagat cattcacgac ctccgcccca
     8101 tcgaaggccg cttcctcagt acggaggacg aggaaacaag cgcttcggtc tgcgtgctgg
     8161 gattgaatgc gaaaatcggg atgctgggct acgaacctgc ggtgggaaaa cacgtaaaag
     8221 tcaacgacac ctggctgcgc gtcgtgggcg tcctggcgga gcaggttgca gactcgggct
     8281 ccggccgcgc cgtcgatcgc aacaacgtaa tctttatccc gatcagcacc ttccgctatc
     8341 gcttc
//
LOCUS       DASTMK010000279        13298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_45478, whole genome shotgun sequence.
ACCESSION   DASTMK010000279 DASTMK010000000
VERSION     DASTMK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45478"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..4620)
                     /locus_tag="VFQ79_12070"
     CDS             complement(16..4620)
                     /locus_tag="VFQ79_12070"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEU0140448.1"
                     /translation="MQIRSAVIGAAFLLLGVPSVAQLDEGREYFSLNTEKTFAPGDKP
                     TVRLWAQGVDTLQFRVYRVKDPVKFFANLPDPHRFGGQPPRPPRELTVLEQFHLWKYR
                     SRTWLRNVVRRQYPAESRRIIRTALAERSQQRVQRGAATATEYADVPVLNKQQLVSVW
                     TQSVPRGNRWDSQTVPVDVKEKGVYLVEATNGNLQAYTIISVTNLAVISKGSPGKISA
                     RVVDRSTGAPLKGCQIVVWSEEKQQAQLETNDDGMAEAGITLANPENVLMLATYRDDF
                     AAGALYGWNLRTDRQMSAYIYTDRPVYRPGHTVYFRAILRLRSPEGYRLPPRSAIQLQ
                     IQDPEGKPVFRRDVTPSAMGTVHGEFKLPEASALGYHSVELRSGEVYIGSGGFHVEEY
                     LKPEYEVRVTPEKSRVLLGEEASATIDARYYFGEPVANAKVTWVVHRSRYFPPYYADE
                     FDSEDPRFQDDGFGNEQILEQTGTLDAAGKLTVRFRSESAPHDLRYRIEARVTDAANR
                     EISGTGFVLGTAGPYFVHIEPEQYVYKPGETAVFRIEARNYDGQAVPNVPFRVELMEQ
                     RDGQESSLFQSTSGQTDASGNARAGLAVRAGSLIARVSSTTPEGRTVQDRTWLWVSGG
                     TEWYGARSQRVEIIPSKKSYRGGEVARLLIVTGVPEAWVWVTAERGTIHSSQLVHAKQ
                     PAFTIDMPIRAEFAPNFYVTATFVRENQFYQGTKSVRVPPVEQEMKVSIKSSAAQFKP
                     GGSGTFMIEATDHQGRPVAAEFSLGVVDEAIYAIRREAAQDILSFFYGRAYNQVSTDS
                     SLNYFFYGEAGKRRMQLARIRQSAARAQLKPQQFVEPEIRKLFPDTIRWIADLRTNSN
                     GRAEAKVEYPDSLTTWRATARGITADTKVGSAIERTIVRKNLILRLAVPRFFTEGDEV
                     TIRAIVQNYLEEQKTVRISLDVKGIEMIEGATRDMTVPSRGEVQADFRVRAKAPGKAV
                     LLGKALTDEESDALELTLPVNPFGVELSESKSGTLSSATGQSEADLTFPVTSNPAARG
                     IEISVSPSVAGTIFGALEYLTSYPYGCTEQTMSSFLPNVLVSRAASELGLRSSIDADE
                     LAKKVRAGLERLYDYQHEDGGWGWWKSDDSEAFMTAYVLSGLARAQAAGYSVRPDTIN
                     RAAQWLRTNPAALRGVSEDLRSYVAYALAEAGTPDKTLLDAVWSNRNSLSPYGLALLG
                     LGLRRAQDGRSEIVADTLEQTARTTDAEAWWEMERDPLLGIAMNSTVEATAFAVKFLT
                     EQRPKSSLLSRAAIYLVNHRNQGYYWSSTKQTAMVVYGLTDYLKLSGELKPDFTATVL
                     VNEKPVLTKRFTAAEALSPGAEPLRIASDQLNPGLNRVRVSKQGTGTLYWSARAVYYM
                     PEAPASTSSSSLRIAREYHRLVPEKTGDRIVYRLEPLTGSVAPGDVIAVRLTVTGHEW
                     KYLMMEDPIPAGTEFIERDELYELSEKPGWWGRWYARREFHDDHAALFQTYFPEGQAE
                     HVYLLKVVSPGQFRVSPARVQPMYEPQHSAATPSRTLEVK"
     gene            complement(4620..5690)
                     /locus_tag="VFQ79_12075"
     CDS             complement(4620..5690)
                     /locus_tag="VFQ79_12075"
                     /inference="COORDINATES: protein motif:HMM:NF018387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HEU0140449.1"
                     /translation="MDGMLKPAFARFRKLPRRRARLLVAVALAAAATLTVAVAGRSRE
                     GSGSLLFEASLERLPADGFSRSTLRVRSQSGEELKASFKVLEGARSVRVAGSSVYARI
                     LPGTALLEARAPGYAPARLRIETVPFFSDRTGDGTPDFLRLDSFDDRDAFRRWFCFIA
                     EAQYFREPAGLSAEVDDCAALARFAYREALRRHDGEWADRLDVPFLPSAPDVGKYHLP
                     HTPLGPKVFRILPGPFEPGDLDGPAYAEFADAQTLMRLNSHFITRDVQQAVPGDLLFF
                     RQLEQDLPFHLMIYIGVSQLEPDSSNRVVYHTGPIGEEKGEIRRPALAELNHHPSPRW
                     RPVPGNSNFLGVYRWNILRGAH"
     gene            complement(5697..7286)
                     /locus_tag="VFQ79_12080"
     CDS             complement(5697..7286)
                     /locus_tag="VFQ79_12080"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0140450.1"
                     /translation="MTPQSLQRRGSQGIGRRQLIAAAGWPLFHAITSRKGLAAEGRTR
                     KLVVHADSEIAAIRPALHGHFAEHLGSCIYGGLWVGRDSKIPNIRGHRKQAVEYLRQL
                     GIPVLRWPGGCFADDYHWRDGIGPAEKRPRRVNLWWGQYTEDNSYGTHEFIDFCRLIE
                     AEPYLAGNVGSGTPQELRDWMEYCNYPDGSTLSGERAANGSREPFRVRYWGVGNENWG
                     CGGRMTADEYATHYRRFANYLTPEGGIGGTKVFLIACGPNRNDTEWSRRFLSSMNRSL
                     AMVHGFAMHFYSSGKSAATKFTNETAREQLSSFADLEKAVVQQHALLRSFDPVGNIGL
                     MVDEWGVWDRIEAADEKRYGKLWQQITMRSAVAAGLGLNVFHRQADKLIMCNIAQIAN
                     VLHSLLLTDEDRCIRTSTYYAFELTKPHRGATAVRVENGDDDPLGISISASRAGGQVV
                     VTTVNPKIDEPARVECVLPGRGVKSVSARLLHHADMNACNTFENPDTIVPRMHQVETT
                     KAGLRFEMPPLSVVTAVIQLA"
     gene            complement(7290..8858)
                     /locus_tag="VFQ79_12085"
     CDS             complement(7290..8858)
                     /locus_tag="VFQ79_12085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0140451.1"
                     /translation="MRAFPNPVQPDDAVYRENLRLNQVEYYLEHIHLLSQPRCLGIVL
                     GNACNLHCVHCYQAKNGDNLLRPAAIGWELRREFMAMYPYLDTLRIQGGEVFAIPEFQ
                     DLVDDVASVAARPLLSISTNGTLIDDVWAERMVRTPFRAVTISIDAAHAATFARLRRG
                     ANLATVLANVAHVQEWKRRLGAAMPVLDSFFVIMRSNFREIPDYLELMKGLGIGEIAL
                     QTMEVNRENSSREPGLEQREAILDPAEVRELHSVLKSAFARYRGAFQMIRVSGVRVLF
                     AAHGLDSSFLEEEQCGLYPESGVFDCGGDGFALCPNPWTTLFVTENGNVHLCFLSEPI
                     GNLYETPLSRLWNSPAAVAKRSDIIAGRYTASGCSRRWCSWRDSSRRTPTDGKRVRQL
                     LAEFEELRRRAASLLPVPPTPAPYPSALPALRRLFTSKDRRIAELECLFRQLCDTNGE
                     IHETGRKYIDELEGQIEALQAHADHLEAKTEKAVADFRMLQGEFQRFRRPLSVRAAHK
                     LAGIWARMTRQRER"
     gene            complement(8865..10031)
                     /locus_tag="VFQ79_12090"
     CDS             complement(8865..10031)
                     /locus_tag="VFQ79_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140452.1"
                     /translation="MAKELRVGMVGYGFMGRTHSNAYKRLNDFFAVERRPVLKAVCAR
                     KPEKAKAFAETWGYERVETDWRKLVEAKDIDLIDIASPNNTHHDIAIAAAQNGKMVLC
                     EKPLAMNTAEAEEMVRTVENAGVPNMVWFNYRRVPSISLAKQIVEEGRTGRPFHYRAT
                     YLQDWTISEDVPQGGPALWRLDVDVAGSGVTGDLLAHSIDTAMWLNGAISRVVAKTET
                     FVKEREHVETGKRQPVGIDDACMFLAEFANGSMGTFESTRYARGRKNFNTFELNGEQG
                     SIYFDLEEPEYLQFFEYKQLQSGRKTESHLTGWRKIHTTNSEHPYMSRYWVPGTTIGY
                     EHTFLNALADFVAGIESGQPTQPDFRNALQTQRVCDSVLESAQSGQWVATGAQV"
     gene            complement(10059..11006)
                     /locus_tag="VFQ79_12095"
     CDS             complement(10059..11006)
                     /locus_tag="VFQ79_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0140453.1"
                     /translation="MSKRTSIGSWAFTIGPYASNPIDFETVCEKLKALGFDGVELGAF
                     PPHPNPGNPSGPDGNWPGAMPEASERRELAARMKERGLGFSGMAANLWGEKLINTDDQ
                     TKYISEFRKNSEFARDVGIQGIRVDCVQPPTIHREIDYKTAMDRVVRTWKTCSDIAAD
                     AGQYVTWEFEPGFAFNKPGDIVRIHDAVDKANFGIQYDTCHGQMVGVNGVRQEGEKET
                     FPSQVEFIRFLSGRINHIHVIDSDNTCHKDAEGNDETSAHPPFGLGVLDFDSIMPELV
                     KAARLSHDWWTIDLCFWPDAWEATARCKTRLDQLVEKYG"
     gene            11207..>13298
                     /locus_tag="VFQ79_12100"
     CDS             11207..>13298
                     /locus_tag="VFQ79_12100"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU0140454.1"
                     /translation="MRKRFIPLIVCFTVAAAAQDVGMGRRKGSGKRFEEPYTLNVQTP
                     HVKWANPLPGGPIKLLAVPSVSEGRTLVELMQRLSLDVTSVTIDSAFDRNKWTMCFGR
                     DYGARAERGDLSLIYSYLEQELTSAKHFDTILLQLNHGWEVLTPKSREALLKRVREGA
                     GLVLLRPMENELSPLAPASAIAPPSRPYNEVEPPSAPAGKWKRIAEHYVTRGIPVETF
                     PFEYLEEYAYRPAAGATVLIESETGRPIAATTSFGKGRIVAFGFHNHGLSWQMPMSAK
                     GFVPDLQWEYYYAMLSRALIYTAGREPQGQFVPSVWRLKTADGTVKRSGKGRPPKSPV
                     TAPGLYFLEQQSASDFEISAIKLGELDRIEQLQTDAGVIGEGQTVNVTWSAEKPARVE
                     LTDGFGRVIARSQGVNSAVLKAGRPLTHSGFILVTAGTGSARLPVQFAASSREWSDYE
                     VIMPWYGPGSYQPWIPALDEQFRQFGLTTLARPDRNFKVIASAGLHDTFGVYAYRNQM
                     YVARKNAYVETKDKKYLTRDVVLQSPDFEANLRRNLEKNLKPLAPLHPLAYYLADESS
                     LTSYTDPFDVDWSPETLAAFRLWLQKEYSSVDALNASWETSFTGWSDVVPMTTEEVQK
                     HGNYAPWTDHRIFMEQDFVRVLGRARDMVREIDPGALASISGTQVPTAHNGCNWYEIN
                     QRMDYLQPYSGGNQD"
BASE COUNT         2609 a         4123 c         3884 g         2682 t
ORIGIN      
        1 gcatgaagcc cggcatcact tcacctccaa agtgcggctt ggagtagccg cggaatgctg
       61 tggctcatac atcggctgca cacgtgccgg acttactcga aattgtccgg gacttacaac
      121 ttttagtaag taaacgtgct ccgcttgtcc ctccgggaag taagtttgga acagagccgc
      181 gtggtcgtcg tggaactcgc gcctggcata ccaacggccc caccatcccg gtttttcgct
      241 tagctcgtac aattcgtcgc gttcgatgaa ctccgttccg gctggaatag gatcttccat
      301 catcaggtac ttccattcgt gccctgttac tgtgagccgt acagcaatca cgtcgccggg
      361 cgctaccgaa ccggtcaaag gctccagccg gtaaacgatg cgatctccgg tcttctcagg
      421 aaccagccgg tggtattccc gtgcgattcg cagtgaggaa ctggaagtcg aggctggcgc
      481 ctcgggcatg taatacacgg cgcgggcgct ccagtacagt gtgccggttc cctgcttcga
      541 aacgcgaacc cggttgagtc ccggattcaa ctggtccgat gcaatgcgca gaggctcggc
      601 gcccggcgag agtgcttcgg cggcggtgaa tcgtttcgtc agtacaggct tctcgttcac
      661 cagcacggtc gccgtgaagt ccggcttcag ttcgccgctc agcttcaagt aatcggtgag
      721 gccataaacc accatggccg tctgtttcgt cgatgaccag tagtagccct ggttccgatg
      781 attcaccagg tagatcgcgg cgcggcttaa tagtgaactc ttcggccgct gctccgtcaa
      841 aaacttcacg gcaaatgcgg tggcctctac cgtgctgttc attgcgatgc cgagcagagg
      901 atcgcgctcc atttcccacc aggcttctgc gtccgtggtc ctcgcggtct gctccagtgt
      961 gtcggcgacg atttccgagc ggccgtcctg cgcgcggcgc aaccccaggc cgagcaacgc
     1021 cagcccgtaa ggagacaggc tgttgcggtt gctccaaacg gcatcgagca gtgtcttgtc
     1081 gggtgttccg gcttccgcga gcgcatacgc tacgtacgaa cgaagatcct cggaaactcc
     1141 gcgcagcgcc gcgggatttg ttctcagcca ttgcgcggcg cggttgatgg tatccggacg
     1201 cacactgtag ccggcggctt gggcgcgtgc caggccggac agcacatagg ccgtcatgaa
     1261 ggcttcgctg tcgtcgctct tccaccatcc ccatccgcca tcttcgtgtt gatagtcgta
     1321 gaggcgttcc aggcccgctc gaaccttctt cgccagctcg tccgcatcga tgctcgaacg
     1381 aagtcccaat tccgaagccg ctcgcgagac gagcacgttc ggcagaaaac tggacatggt
     1441 ctgttccgtg cacccgtatg ggtaggaggt aaggtactcc aacgcgccaa agattgtccc
     1501 cgcaaccgaa gggctcacgg agatctcgat gccgcgagcc gcgggattgg aggtgaccgg
     1561 gaacgtcaga tccgcttccg actgtcccgt agccgatgac agggttccgg acttcgattc
     1621 ggacagttcc acgccgaacg ggttcacggg cagcgtcaac tcgagcgcgt ccgattcctc
     1681 atccgtcagc gccttgccca gaagcacagc cttgcccgga gcctttgccc gtacgcggaa
     1741 atcggcctgc acctcgccgc ggctgggcac cgtcatgtcg cgcgtggcgc cctctatcat
     1801 ctctatgcct ttcacatcga gtgaaatgcg aaccgtcttc tgctcttcca aataattctg
     1861 gacaatggcc cggatggtga cctcgtcgcc ttccgtgaag aatcgcggca ccgccagccg
     1921 cagaatcagg tttttgcgga cgatggtacg ctcgatcgcg ctgccaacct tcgtgtcagc
     1981 ggtgatgccg cgcgcggtag cgcgccacgt ggtgagcgaa tccgggtatt ccaccttggc
     2041 ttccgcgcgc ccgttggagt tcgtgcgcag atcggcaatc caccggatcg tatcgggaaa
     2101 cagcttccgg atctccggct cgacaaactg ctgcggcttc aactgggctc gcgccgcgga
     2161 ctgacggatg cgcgccaact gcatccgccg ctttccggct tccccgtaga agaaatagtt
     2221 gagcgacgag tctgtactca cctggttgta agccctaccg taaaagaacg aaaggatgtc
     2281 ctgtgcggct tcgcggcgaa tagcgtaaat cgcctcgtcg accactccga ggctgaactc
     2341 cgccgccacg ggccggccct gatggtcggt ggcctcgatc atgaaggtgc cggatccgcc
     2401 gggcttgaat tgcgctgcgg aagatttgat cgagaccttc atctcctgtt ccaccggtgg
     2461 aactcggacg ctcttcgttc cctggtagaa ctggttctca cgaacaaacg tggccgtgac
     2521 ataaaagttg ggagcgaatt ccgcgcggat cggcatatca atggtgaacg ccggttgttt
     2581 ggcatgcaca agctgcgagc tgtgaatggt tccgcgctcg gcggtcaccc agacccaggc
     2641 ctcgggtact cctgtgacga taagcaggcg agcgacctcg ccgccgcgat acgacttttt
     2701 gctgggaata atctccacac gctgagaccg cgcgccgtac cattcggtgc ccccgctcac
     2761 ccatagccac gttcggtcct gcacggtgcg gccctcgggc gtcgtcgagc ttacgcgggc
     2821 gatgagcgat cccgctcgaa ccgccagccc cgcgcgcgca tttccgctgg catccgtctg
     2881 gccgctggtg gactggaaca agctgctttc ctgcccgtct cgctgctcca tgagctcgac
     2941 acgaaacgga acgttcggaa ccgcttgccc atcgtagttc cgcgcttcaa tccggaatac
     3001 agccgtttca ccgggtttat acacgtactg ttcgggctcg atgtgcacaa aatacgggcc
     3061 ggcggtcccc agtacgaatc cggtccccga aatctcccgg ttggcggcat ccgtgacgcg
     3121 ggcctcgatg cgatagcgca gatcgtgcgg agcggattca ctccgaaaac ggacggtgag
     3181 ctttccagcc gcgtcgagag tgccggtctg ttcaaggatc tgttcgtttc caaacccgtc
     3241 gtcctggaac cggggatcct ccgagtcgaa ctcgtcggca tagtagggag ggaagtatcg
     3301 cgagcggtga acgacccacg taaccttggc atttgccacc ggctcgccga aatagtagcg
     3361 cgcgtcgatg gtggccgagg cttcttcgcc aagtagcacc cggctctttt ccggcgtaac
     3421 acggacctcg tattcgggct taagatactc ttccacatga aacccgccgc ttccgatgta
     3481 gacctcgccg ctgcgcagtt ccaccgagtg atagcctaac gccgaagcct cgggcagctt
     3541 gaactcacca tgcaccgttc ccatcgccga cggcgtcaca tcgcgtcgaa agaccggctt
     3601 gccctccgga tcctgaatct gcaactgaat ggcgctgcga ggcggaaggc ggtagccttc
     3661 gggcgatctc aatctgagaa tggcgcggaa ataaaccgtg tgacccggcc ggtaaaccgg
     3721 gcggtccgta tagatgtaag ccgacatctg ccggtccgtt cgcaaattcc aaccgtagag
     3781 tgctccggcc gcgaagtcgt cgcggtaggt ggcgagcatc agaacatttt cgggattcgc
     3841 taaagtgatg ccggcttccg ccatgccatc gtcgttggtt tcgagttggg cttgctgctt
     3901 ttcctccgac cagacaacga tctgacatcc cttcaaggga gcccctgtgg agcgatcgac
     3961 aacgcgcgcg ctgattttgc ccggcgatcc tttggaaatg acggcgagat tcgttaccga
     4021 gatgatagtg taagcctgta aattcccgtt cgtcgcttcc accaggtaga cacccttctc
     4081 tttcacatcc acgggtacgg tttgggaatc ccagcggttt ccccgcggga cgctctgtgt
     4141 ccagacggaa acgagttgct gcttgttcag caccggcacg tccgcatatt cggtagcggt
     4201 ggccgcgccg cgctgtacgc gctgctgact ccgttcagca agcgccgttc gtataatccg
     4261 gcgcgactcg gccggatatt gccgccgtac gacgttgcga agccaggtgc gcgaccggta
     4321 tttccagaga tggaactgct ccaaaacagt cagctcgcga ggaggacgcg gaggctggcc
     4381 gccaaaccgg tgagggtcgg gcaggtttgc gaaaaacttt accgggtcct tcacgcgata
     4441 tacgcgaaat tgcagcgtgt cgacgccctg cgcccataac cggacggtgg gcttgtcgcc
     4501 cggagcgaaa gtcttctccg tgttcagcga gaagtactcc cgcccttcgt ccaattgcgc
     4561 cacagacggc acgcccagca acaggaatgc tgcgccaatg actgccgaac gaatctgcat
     4621 caatgagccc ctcgaagtat attccaacga tacacaccaa gaaagttgct gttgccaggc
     4681 accggacgcc accggggaga gggatggtga ttgagctcag cgagcgccgg gcggcgaatc
     4741 tcccctttct cttcgccaat gggacccgta tgataaacga cgcggttcga cgagtccggc
     4801 tccagttgac tcaccccgat atagatcatc aggtgaaacg ggagatcctg ttccaactga
     4861 cggaagaaaa gcaagtcgcc gggtaccgcc tgctgaacgt cgcgcgtgat gaagtgcgag
     4921 ttcagccgca tcagcgtttg tgcatcggca aactcggcgt atgccggccc gtcgagatca
     4981 ccgggctcga agggaccggg aaggatccgg aacaccttcg gtcccaaggg agtatgcggt
     5041 aagtggtact tccccacgtc gggcgcggag ggcaggaacg gaacgtccag cctgtccgcc
     5101 cattcgccgt catgccggcg cagcgcttcg cgataggcga agcgggccaa cgccgcgcaa
     5161 tcgtccactt ccgccgatag accggcgggc tcgcggaaat attgcgcttc ggcgatgaaa
     5221 caaaaccatc ggcgaaaagc gtcgcggtcg tcgaaagagt cgagacgtag aaagtctggt
     5281 gtaccgtcgc ccgtgcgatc gctaaagaaa ggaaccgtct cgatacgcag acgagcagga
     5341 gcatatcccg gagcgcgcgc ttccaggagc gctgttcccg gcagaatccg cgcgtacaca
     5401 ctcgaaccgg caacacgcac cgaccgtgca ccttccaaca ctttgaagga agccttcaac
     5461 tcctcgccgg actgcgagcg cacgcgcaaa gtcgagcgcg agaagccatc ggcaggcaga
     5521 cgctccagcg aggcctcgaa aaggagtgac cccgatcctt cccgagagcg tccggccaca
     5581 gctacggtta gcgtggcggc cgctgcgaga gctactgcta caagcagacg tgcgcgccgg
     5641 cgaggcagct tccgaaaacg agcgaacgcc ggtttcaaca tcccatccat tcctcgttaa
     5701 gcaagctgta tgaccgccgt gacaacggaa agcggcggca tctcgaaccg caagccggcc
     5761 tttgtggtct caacctggtg catgcgcggc acgattgtgt ccgggttctc gaacgtgttg
     5821 caggcgttca tatccgcatg atgcaacaga cgtgcggaga cggacttcac accacgtcct
     5881 ggcaaaacac attcgacgcg cgccggctca tcaattttgg ggtttaccgt cgtaaccacg
     5941 acttgcccgc cggctcgcga cgcggatatc gagatgccga gcggatcgtc gtcgccgttt
     6001 tccacccgta cggcggtggc gccgcggtgc ggcttcgtca actcgaaagc gtaataggtg
     6061 gaggtgcgaa tgcaccggtc ttcgtccgtc aacagcagtg agtgcagcac gttggcgatt
     6121 tgcgcgatgt tgcacatgat gagtttgtcc gcctggcgat ggaagacgtt cagccccagc
     6181 ccggcggcca ctgcactgcg catggtgatc tgttgccata gcttgccgta gcgtttctcg
     6241 tccgcggcct ctatgcggtc ccacacaccc cactcgtcca ccatcagccc gatgttgccc
     6301 accgggtcga aactgcgcaa cagcgcgtgc tgctggacga cggctttctc cagatccgca
     6361 aaactggata actgttcgcg cgccgtctcg ttcgtgaatt tcgtcgccgc gctcttgcca
     6421 ctcgaataga aatgcatggc gaacccgtgc accatagcga gcgaccggtt catcgacgaa
     6481 agaaaccggc ggctccactc cgtatcgttg cggttcggcc cgcacgcgat caggaatact
     6541 ttggtgccgc cgatgccgcc ctccggagtg aggtaattag cgaagcggcg atagtgggtc
     6601 gcgtactcat ccgcggtcat tcgcccgccg caaccccagt tctcgttccc gacaccccag
     6661 tagcggacgc ggaacggctc cctggatccg ttggcggcgc gctcgccgga aagcgtgctg
     6721 ccgtcgggat agttgcagta ttccatccag tcgcgaagtt cctgcggcgt gccactgccg
     6781 acattgcccg ccaggtatgg ctcagcttcg atcaggcggc agaagtcaat gaattcgtgc
     6841 gtcccgtaac tattgtcttc cgtgtactgg ccccaccaca ggttcacgcg gcgcggtctc
     6901 ttctccgcgg ggccgatgcc gtcgcgccag tggtagtcgt cggcgaagca gccgccaggc
     6961 caacgcagca caggtatgcc aagctgccgc aaatattcaa ccgcctgttt ccggtggccg
     7021 cggatattgg gaattttcga atctctgcca acccacaagc cgccataaat gcaggagccg
     7081 aggtgctcgg cgaaatgtcc atgcagagcc gggcggatcg cggcgatttc cgaatcggcg
     7141 tggacaacga gttttcttgt gcgtccttcg gccgccaacc cctttcgaga cgtgatggca
     7201 tggaacagcg gccagccggc cgccgcgatc agttggcgcc tgccgattcc ctggctgccg
     7261 cggcgctgta gcgactgtgg agtcatggtt caccgctccc gctgtcttgt cattctcgcc
     7321 catatgccgg ccagcttgtg tgcggcgcgc acagacaggg gccgccggaa gcgctggaac
     7381 tcgccttgca acatccggaa atccgccacc gctttttccg tcttggcttc gaggtggtcc
     7441 gcatgcgcct gtaatgcttc gatctgcccc tccagttcat cgatgtactt ccggccggtc
     7501 tcgtggatct cgccgttcgt atcgcaaagc tggcggaaca ggcattcgag ctcggcgatg
     7561 cggcggtcct tggaagtgaa tagccgccgc agcgcgggca acgccgaagg gtatggagcc
     7621 ggggtgggcg ggacgggcag gagcgaagcg gctctccggc gcagttcttc gaactcggcc
     7681 agtaattgcc tgacgcgctt tccgtccgtt ggagtacgcc ggctgctatc cctccaggaa
     7741 caccagcggc gcgagcagcc agaagcggtg taacgcccgg cgatgatatc gctgcgcttg
     7801 gctaccgcgg ctggcgagtt ccataaacgc gacagcggag tttcatacaa attaccgatg
     7861 ggctcggaca aaaaacaaag atgcacattc ccgttttccg tcacgaagag cgtggtccac
     7921 ggattcgggc aaagcgcgaa gccgtctccg ccgcagtcga acacgccgct ctccggatag
     7981 agaccgcatt gctcctcttc gagaaacgaa gagtccaggc cgtgcgcggc aaacagcacg
     8041 cgaactccgc tgacacggat catttgaaac gcgccccgat accgggcgaa cgcgctcttc
     8101 agaaccgagt gaagctcccg cacttcggcc ggatccagga tggcctcgcg ctgctccagt
     8161 cccggttccc gcgacgaatt ctcccggttg acctccatcg tctgtagggc gatctcgcca
     8221 atcccgagcc ccttcatcag ctcgagatag tcggggatct cccggaagtt cgaccgcatg
     8281 atcacgaaaa acgaatccag aaccggcatg gcggctccaa gccgccgctt ccattcctgg
     8341 acatgcgcca cgttcgcgag aaccgttgcg agattcgctc cgcggcgcag ccgcgcaaag
     8401 gtggcggcgt gcgccgcatc gatggagatg gtgaccgcgc ggaacggcgt ccggaccatc
     8461 cgctcggccc acacgtcgtc gataagcgtg ccatttgtgc tgatgctcaa cagcgggcgg
     8521 gcggccaccg aagcgacgtc gtccacaaga tcctggaact ctggaatggc gaacacttcg
     8581 ccgccttgaa ttcgcagcgt atccaggtac ggatacatcg ccatgaattc ccggcgcagt
     8641 tcccacccga tggcggcggg ccgcagcagg ttatcgccgt tctttgcctg atagcagtga
     8701 acgcaatgga ggttgcaggc attccccagc acgattccaa ggcatcttgg ctgcgagagc
     8761 aggtggatgt gttccaggta gtactccacc tggttcaacc gcagattttc gcggtaaacc
     8821 gcatcatccg gttgaacggg gttggggaat gccctcatgc agcgctacac ttgagctccc
     8881 gtcgctaccc attggccgga ctgtgccgat tcgagaaccg agtcgcaaac acgctgcgtc
     8941 tggagcgcat tgcggaaatc gggctgagtc ggctgtcccg attcgatgcc ggccacgaaa
     9001 tcggccagag cgttcagaaa cgtgtgctcg tagccgatgg tggttccggg cacccaatag
     9061 cggctcatat aaggatgctc cgaattggtc gtgtgaatct tgcgccagcc ggtcaggtgc
     9121 gactccgtct tcctgccgga ctgaagctgc ttgtactcga agaactgaag atactccggt
     9181 tcctcgaggt cgaagtagat gctgccctgc tcaccattca actcgaacgt gttgaagttc
     9241 ttgcgcccgc gcgcgtaacg cgtggattcg aacgtgccca tcgagccgtt cgcaaactcc
     9301 gccagaaaca tacaggcgtc gtctattccc accggctgcc gcttgcccgt ttccacatgc
     9361 tcgcgctctt tcacgaaggt ttcggttttt gcgacgaccc ttgagatcgc cccattcagc
     9421 cacatggcgg tatcgatgga atgagccaga aggtcgccgg taacccccga tccggcgaca
     9481 tccacatcca ggcgccacag cgcgggccct ccctgcggca cgtcttccga gatggtccaa
     9541 tcctgcaaat acgtcgcgcg atagtgaaat ggccggcccg tgcggccctc ctcgacaatc
     9601 tgtttcgcca gcgagatgga aggcacacgg cggtagttaa accagaccat attcgggacg
     9661 cccgcgtttt ccacggttcg caccatctcc tcggcttcgg cggtattcat ggcgagaggc
     9721 ttctcgcaca gcaccatctt gccgttttgc gcggccgcaa tagcaatatc gtggtgtgtg
     9781 ttattgggcg aggcgatgtc gatcagatcg atatctttcg cctccacgag ttttcgccag
     9841 tccgtctcca cgcgctcata gccccaggtt tcggcgaacg cttttgcctt ctcgggcttc
     9901 ctcgcgcaaa ccgccttcag aaccgggcgg cgctcgacgg caaagaaatc gttcaaccgc
     9961 ttataggcat tcgaatgagt ccgtcccatg aagccgtagc cgaccattcc gacacgcagt
    10021 tccttcgcca tttccgatat ctcccttggg gtttgcgctc aaccgtattt ttcgacgagc
    10081 tgatccagcc tggttttgca gcgcgccgta gcttcccacg catcgggcca gaagcacaga
    10141 tcgatggtcc accagtcgtg cgacaggcga gcggccttca ccagttccgg cataatgctg
    10201 tcgaagtcga gaacgcccaa tccgaacggc gggtgcgcgg atgtctcgtc gttgccttcg
    10261 gcgtctttgt ggcaggtgtt gtcggagtcg ataacgtgaa tatgattgat gcgcccggaa
    10321 aggaaccgta tgaattccac ctggctgggg aacgtctcct tttccccttc ctgccgcaca
    10381 ccgtttacgc ccaccatttg cccgtggcac gtatcgtact gaatgccgaa attggcttta
    10441 tccaccgcgt cgtgaatcct cacgatatcg cccggcttgt tgaacgcgaa tcccggttcg
    10501 aattcccagg tgacgtactg tccggcatcc gccgcgatat cgctgcatgt tttccaggtt
    10561 cgaaccacgc gatccattgc cgtcttgtaa tcgatctcgc gatggatggt tggaggctgc
    10621 acacaatcga cgcggatgcc ctggatcccc acgtcgcgcg cgaactccga gttcttccgg
    10681 aattcggaaa tgtattttgt ctgatcgtcc gtgttgatca gcttttcccc ccaaagattc
    10741 gcagccatgc cgctgaatcc aagcccccgc tccttcatgc gcgcagcgag ctcccgccgc
    10801 tctgaggctt ccggcatggc ccccggccag ttgccgtcag gaccgctcgg attgccggga
    10861 ttcggatgag gtgggaacgc gccaagctcc acaccatcga atccgagggc cttcagcttt
    10921 tcacacacgg tttcaaaatc gataggattg cttgcatagg ggccgatggt aaaggcccag
    10981 gaaccgatcg aagtacgttt gctcaaacta ttcctccgct ggtgcgtaag atgcggggca
    11041 ccaactgtta cgggtcccgc acgatcaata ctggaatatc gcaaatgggt tgaatataca
    11101 agccgaaccg gacggctggc aacggcttca ttttcggttc cctgcgctca agcgatagaa
    11161 tcgttgttgt tcgaaaatta gatatcaggg tcttctttac atatccatga gaaaacgatt
    11221 cataccgcta atcgtctgtt ttacggttgc cgccgccgcg caggacgtcg gcatgggccg
    11281 ccggaaagga agcggaaaac gcttcgagga accttacacg ctcaacgttc aaaccccgca
    11341 cgtaaagtgg gccaacccgc tgcccggcgg acccataaaa ctgctcgccg tgccgtcggt
    11401 cagtgagggg cgcactttag tggagctgat gcaaaggttg tcgctggatg tgacgtcggt
    11461 caccatcgac tccgccttcg acagaaataa atggacgatg tgctttggac gcgactacgg
    11521 cgcccgtgcc gaaagaggcg acctgagtct gatctattcg tacctggagc aggaactgac
    11581 gtcagcgaag catttcgata cgatcctgtt gcaactgaac cacggatggg aggtgctcac
    11641 acccaagtcc cgggaggcgc ttctaaagcg cgttcgcgaa ggtgccggac tggtgttgct
    11701 gcgtcccatg gagaatgagt tatcgccgct tgcgcccgct tcggcgatcg cgcctccgtc
    11761 caggccatac aacgaagtgg aacctccgtc tgcgccggcc ggcaaatgga agcgaattgc
    11821 ggagcactac gttacgcgag ggataccggt tgaaaccttc ccattcgaat acctggagga
    11881 atacgcctac cgcccggctg caggagctac ggtgctgatc gaatcggaga ccggccggcc
    11941 tatcgcggcc acgacctcat tcggcaaggg gcgaattgta gcgttcgggt tccacaatca
    12001 cggcctgagc tggcagatgc cgatgtcggc gaaaggcttc gttccggatc ttcagtggga
    12061 gtattactac gccatgctct cgcgggcgct catttacacg gcgggaagag agccgcaggg
    12121 ccagttcgtt ccgtccgtct ggcggctgaa aaccgccgat gggacggtga agcggtccgg
    12181 caaaggccgt ccgcctaaat cgccggttac tgcgccgggg ctatacttcc tcgagcagca
    12241 gtccgcgtcc gatttcgaga tttctgccat taagcttggt gagctcgaca ggattgagca
    12301 actccagacc gatgccggcg taattggaga aggccagacg gtaaacgtca cctggagcgc
    12361 tgagaaaccc gcgcgcgtcg agttgacgga tggcttcggc cgcgtcatcg cccgctcgca
    12421 gggtgtgaat agtgcagtgc tcaaggcagg ccggccgctc acgcactccg gatttatttt
    12481 ggtgaccgcg ggcacaggca gcgcccgcct tcccgtgcag ttcgccgcat cctcgcgcga
    12541 atggagcgac tacgaagtga tcatgccgtg gtatggaccc ggttcctacc agccctggat
    12601 tcccgccctg gatgagcagt tccgccaatt cgggcttacc acgctggcac gccccgaccg
    12661 gaacttcaaa gtgatcgcca gcgcggggct gcacgatacc ttcggcgtct atgcgtatcg
    12721 aaaccagatg tacgtcgccc gcaagaacgc gtatgtggag acgaaagaca agaaatacct
    12781 gacgcgtgat gtcgtgctgc aatcgccgga tttcgaggcg aacctgcgcc gcaatctcga
    12841 aaagaacctg aagccgctcg cgccgctgca cccgctcgct tactatttgg ccgacgaatc
    12901 gtcgctaacc agctacacag accccttcga tgtcgattgg tcaccggaaa cgctggcggc
    12961 gtttcgcttg tggctccaga aggagtattc gtccgtggat gcgctgaatg catcctggga
    13021 gacttccttc accggatgga gcgacgtggt tcccatgacg accgaggagg tgcagaaaca
    13081 cggtaactac gcgccgtgga ccgaccaccg cattttcatg gaacaggatt tcgtgcgtgt
    13141 gctcggcagg gcgcgggaca tggttcgcga gatcgacccc ggagctcttg cttccatttc
    13201 cggcacgcag gtacccaccg cgcacaatgg ctgcaactgg tacgagatca atcaacgcat
    13261 ggattatctg caaccctatt ccggcgggaa ccaggacc
//
LOCUS       DASTMK010000280        11649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_333521, whole genome shotgun sequence.
ACCESSION   DASTMK010000280 DASTMK010000000
VERSION     DASTMK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11649
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333521"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..2240
                     /gene="fusA"
                     /locus_tag="VFQ79_12105"
     CDS             141..2240
                     /gene="fusA"
                     /locus_tag="VFQ79_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0140455.1"
                     /translation="MKAYDGKDIRNVALIGHGNSGKTSLAAGMLYTTGATNRLTSTVE
                     GNTITDFDDEEIQRRITISTAVAYAEWNRSKINLLDSPGFNIFINDTKAVLVAADAAL
                     VVVDGVAGVEVQTEKVWGFCQEFGLPRALFINKLDRERASFDRALESIHEVFGRTAIP
                     IQLPVGAEKDFRGVVDLIRMKCFTYEPGGNGKGKETSIPAELTQAAESAHEALVEMVA
                     EGNDALMEEFFDKGTLPVDRIVEGLRQAILDQRLFPVLCGAAMHNIGTDLVLDFIVEN
                     FPAPVDRGEVKGQNAEGQEVTRKIAESEPLSIFAFKTVADPFAGRLTYFKVYSGVLKN
                     DANLANVRTGSTERFAHIGCLLGKNITPVTELRAGDIGGVAKLKDTLTGDTLTEKGVS
                     VSYPKVELPEPSIAYAIEAKSRQDEDRMGNALQRILEEDASLRFYRDPQTKEFLLAGS
                     GQQHLEVVVSRMKRRYNVDVALKAPKIPYRETIRGTADVQGRHKKQTGGHGQFGDCKI
                     RMEPLPRGEKFEFVNDIFGGAIPKQYIPAVEKGILEAAERGYMAGYPVVDFKVSLYDG
                     SYHDVDSSEMAFKLAGRKAFKAAMEQAKPALLEPIMNVEVSAPVEFAGDLMGDMNGRR
                     GRISGMETKGNTQIIRAQVPMAEMLNYQNDLISMTQGRASFTMEFDHYDFVPGMQSEK
                     IVAQARAAQAGVEEEEE"
     gene            2334..2963
                     /locus_tag="VFQ79_12110"
     CDS             2334..2963
                     /locus_tag="VFQ79_12110"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0140456.1"
                     /translation="MGRKHVSWMFLLGTLLQFHTAGAQSAGFESLTPRKEVTEYWVIV
                     GTPSEVWSIQDGVIACTGKPSGYLRSKRKYKNYILRAEWRFETEGWSSAPPRWPNAGF
                     FIHASEEVGKVWPKSFVEVQGHYGEAGSLFSGGIKGAKRGPIVKDRVPFGAWDRYEIT
                     SRDGTVKVVLNDVLVNEGWGVDPPEGYICLQSEGWPVFYRNIEIKVLPD"
     gene            2990..4144
                     /locus_tag="VFQ79_12115"
     CDS             2990..4144
                     /locus_tag="VFQ79_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140457.1"
                     /translation="MGNYLDRRRLLLSGAAVAASSSMTAQDQEIRAAFIGVGVRGGGL
                     LKQAIGQQAVRISAICDIDGQTRDQALSLSARDNPKSLTEWRAVLDLKDVDAVFIGTP
                     CDLHAEMAAACLEAGKYVYCEKPLAVTAEGVDRVLRAARRSKTFLQIGQQLRYFPSMR
                     EAIRQIHEGIAGKTYIIKAHRHGGRSRPRGGEAGPTPRQATRTAWYADVKRSGDLIVE
                     NAVHNIDACNWIANSRPVSAYGHGKKYFPQPISPGKVMMDGFSVEYIYENDMHCDYSQ
                     ISFHPRHLKALPSGMWYTVFGEKGSVYLTHSSATYYDLFAENEPRNLLPENTSESDTA
                     TADFFACIREKRQPFADVRVGAVAALTAIMGREAIYKGRSVSWKDLGVEV"
     gene            4182..4844
                     /locus_tag="VFQ79_12120"
     CDS             4182..4844
                     /locus_tag="VFQ79_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0140458.1"
                     /translation="MKTLFIALAILVPACVPVPAQAQRPAERGLGPGWVPLFNGKDLT
                     GWKVVGPERWIVEDGTILGEGIHGKDGFLKTEKTYKDFHAYLRFKCETPNNSGFFYHS
                     DIEEDISKIRFIQVEISSSIGGHTGGLHGDHTPETKGRGWIVWPAPELETVIRPFDWN
                     EMLVMVEGKRTRTYLNGVPMIDFTFPDPRNTDGIIALQIHAGKPSRIRFKDIWIRDLT
                     VR"
     gene            complement(4961..7285)
                     /locus_tag="VFQ79_12125"
     CDS             complement(4961..7285)
                     /locus_tag="VFQ79_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU0140459.1"
                     /translation="MAADTPPNQWVELVRDPSGGRRGSAVRYAPDAGAFFLWGFFDHD
                     PEFLQEHPLMEVPEYDVVAFDPGIGRWQNQLPKSREREWSRKLPLAYVPRTYSAITSG
                     SERTVLRGATNDAPGVPRPDLNIVFDQVAWHPRMKSLLYFTGGLTAAYDTAGRRWTDL
                     RPLHSPPPVVGGSLAYDSVNGEMVLFGGGHVAESDRSGNLTGYTGTWVLRDRDWLLLP
                     AGVQPPPRMNTRMVADTRNGVLVLFGGDGQSHFLADTWLYDMKTRSWRVSKAPAGPQG
                     RAGHFTVYDPETGWVIIGGGYNQRDLTDMWAYDAAADRWWELDAKVPAGFYITADIAS
                     DKRLIVLLTNNRRPDDRMTCNVLYPVRTTYGFRIDSKALRAAVKPAAVRHEPIAKRVN
                     ANKPSASARLDSLPLNQWVLLNNTQESAPVRTWGSATFDTARGEILYWGGGHCGYGGS
                     DVDAYSPETNRWRFADDAPEFPERAWDLGVRSAGVTFRGSPWTEHGRSIYAHDPVSGK
                     MIMVIAIRLTTGYDPEALRDYPAKRTVAPDAVVQTPSSYTRQATFSYDTQSGRWELAG
                     PAPLGVDTLVSTPHGVMGVNVNWRSRLNDAGYQRPWSPQDPPVDNTVYLFNLTGKRWT
                     RLGSEQPSPQNLYELTSLAYDTKRDRLMLHGGGARRNELWAFDFRTRRWIHLEPAGGS
                     PAASREAVYLPAADVLLISSPAPEDRSVLALWAYSPDENRWRRVAASFAGRAPRGAAG
                     QNRAMVYDPKHDLILLVLGESLGKAAVYGMRYSP"
     gene            complement(7353..9368)
                     /locus_tag="VFQ79_12130"
     CDS             complement(7353..9368)
                     /locus_tag="VFQ79_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0140460.1"
                     /translation="MSKLGVLLCAAAVFTSLSCRPPEQKTVRQLNVVLVTIDTLRPDR
                     LGCYGHPDNQTPNLDSLARKGALFENAIAQTPLTAPSHASIFTGQNPTVHKVRDTGGF
                     VLPSSSPVLAETLQSRGWDTAAFVGASVLKKGFGFHRGFRVYDDQMPKPAASGMGSEL
                     PERRAGEVVDRALGWLNSQSGKPFFIWVHVFDPHSPYDPPSPFREKYAGRRYDGEVAY
                     TDQQLGRLFDAVGRKSSPEDTLIVVLSDHGESLSEHGEYTHGVFLYDSTLRLVFIIAG
                     PGIPPGQRLKQQARSIDILPTILDLMGSQAPAGVQGISLAPTLMGKQLPATYSYAETL
                     FPKINMGWTELRGIRTNQWKYIRAPKPELYDLVQDPAETRNVIADHPNEVREMEARLA
                     EFAGPSGNRNPEIVGTVVVDQRTMEQLKSLGYSAGMAQQEYRLTGEGVDPKDRLELMK
                     LLHLAVSPDAPAPAALRLGWLRQALAKDPVNPTVYLHLGDEYQRAKRLKEAMQLYQDG
                     IRNGVRTAWIYARLGHLYLQQGRREEAVVAYERAAQLNPTDCESLSDLGLIYLDMERT
                     RDAERVFQWCLASGEEYALTYNGLGLVAVRRQDMIAARGHFEKAVHFDPNLLEAYLNL
                     GRIYRMTGANTRARECFETFLVKAAPAEYGDTIARVRAELEAMREIK"
     gene            complement(9410..>11649)
                     /locus_tag="VFQ79_12135"
     CDS             complement(9410..>11649)
                     /locus_tag="VFQ79_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140461.1"
                     /translation="SGTGDPNTRWSDQLGIPNPNNVAGWPALYSHGLDMDFETENTQT
                     TPFAFYIIDDNATKIVGRHEFQFGFHYRWDKLDTLPEQQYTQGQANGSSIFTSLYDPT
                     TSRNNPLPTQQTGYNLASSYLGLMNYYVQLGHPLFKMRAQEYALYFQDNFKVNSRLTL
                     NLGLRYDYWPANREVSNLLTSFDPTQRAIVLGSDLETMYRLGATLPSVVERMQSLGVK
                     FIGYEEAGLPKSLYRVPKTNFGPRAGFAYRFGSNDRPMVLRGGYRISHFTIPLRAWTA
                     QQRSNAPYYARLTSNINDSALSPDGIANYYMRSAPTIVAGVNSGDAISLSNVSSIVRG
                     SPFASYFAQNQPDTRVHDWNLTLEKEVIPNTVVRASYVGNHTSNLELLYDYNGATPEY
                     IWYATQRQLLPTGEYANVARRFYDQTVYGTVQEYRKDGWGNFSGVTLEVERRYSGGYG
                     FQLFYNVGNALEAGGEQWRISVPELNSYLPGAVPADFHERARLLHYRRDADVPKHRLR
                     WNWIADLPFGRGKALGRNSSGMLDKLIGGWQIAGMGSLRSTYFALPTGVYPTGDKVEL
                     YGYQYPIEDCRSGACRPGYLWWNGYIPASQINSHDASGKPNGIMGVPDNYKPAAQPLI
                     PWGSTVLPPNAPANTNVASFWDTNTVWIPLENGTVQRTTFNDNLHPWRNQYLPSVRQW
                     GLDASLFKTIAFGEQVRVRFNADFFNVLNAPGNPNSVGSNGVLNTFNSGQPARELQLT
                     LRLTW"
BASE COUNT         2460 a         3358 c         3468 g         2363 t
ORIGIN      
        1 cggcgacttc tgtgctaaca tcctcgtttt aagaactctt cattgcagac gcgctgaagg
       61 acctccagaa cagtacggat attaactgcc gctagaccct tgcgcgcctg acatcgaaaa
      121 cagggaaggg gaatcatcgc gtgaaggcat atgatggcaa ggatatccgg aatgtcgccc
      181 tgatcggtca cggaaattca ggcaagacat ccctggcagc cggcatgctt tatactaccg
      241 gcgcaacgaa tcgtctcacc agcacggtgg agggcaacac catcaccgac tttgacgatg
      301 aggagataca gcgccggatc accatctcca cggccgtcgc ttatgcggag tggaacagat
      361 cgaaaatcaa cctgctcgat tcccccggat tcaatatctt catcaacgac acaaaagccg
      421 tcctggtggc tgccgacgcc gccctggtgg tagtggacgg tgtggccggt gtagaagtgc
      481 agaccgaaaa ggtctggggg ttctgccagg aatttggcct tccccgcgcc ctgttcatca
      541 ataagctgga ccgtgaacgc gccagcttcg atcgcgcgct cgagagcatc cacgaagtgt
      601 tcggacgcac cgccattccc atccagttgc cggtcggagc cgaaaaggat tttcgcggcg
      661 tcgtggactt gatccgcatg aagtgtttca cctatgagcc gggcggcaat ggcaagggga
      721 aggagaccag catacctgcc gaattgaccc aggcggccga aagcgcgcac gaggcgctgg
      781 tggagatggt tgcggaaggc aacgacgcgc tgatggagga gttcttcgac aagggcacgc
      841 ttccagtgga ccgtattgtc gaaggattgc ggcaggccat cctcgatcaa aggttgttcc
      901 cggtgctctg cggcgcagcg atgcacaaca tcggtacgga cctggtgctg gatttcatcg
      961 tcgagaattt tcccgcgccc gtggatcgcg gcgaagtgaa agggcagaac gcggaagggc
     1021 aggaggtcac gcggaaaatc gcggaaagtg agccattgtc tatttttgcc ttcaaaacag
     1081 tcgccgatcc atttgcgggg cgtctgacct atttcaaagt gtactcaggc gtattgaaaa
     1141 acgacgcgaa cctggctaat gtccggacgg gctccacgga acgctttgcc catatcggtt
     1201 gtctgctggg caagaacatc acgccggtaa cggaactgcg cgccggcgac atcggtggag
     1261 tggccaagct gaaagacaca ttgactggcg acactctcac ggaaaaaggg gtatcggtgt
     1321 cttaccccaa agtggaactc ccggagcctt ctatcgccta cgccattgaa gccaagtcgc
     1381 gccaggatga ggaccggatg ggcaacgctc tgcagcgaat tctggaagag gatgcttccc
     1441 tgcggttcta ccgggaccct cagacaaagg agtttctgtt ggccggcagc ggacagcagc
     1501 acctggaagt ggtcgtcagc cggatgaaaa ggcgctacaa cgtggacgtc gccctgaaag
     1561 ctccgaagat cccctaccgg gaaacgatac ggggcacggc agacgtgcaa ggacgccaca
     1621 agaaacagac aggaggccat gggcagttcg gcgattgcaa gattcggatg gagccgctgc
     1681 cgcgcggcga gaaattcgag ttcgtgaatg acatttttgg cggagccatt cccaagcagt
     1741 acatcccggc tgtggaaaag ggcatactgg aggccgccga acgcggatac atggccggat
     1801 atcccgtggt ggatttcaaa gtgagcctgt acgacggctc ctaccacgac gtcgattctt
     1861 cggaaatggc tttcaaactg gcgggccgca aagctttcaa agcggcgatg gaacaggcca
     1921 aaccagcctt gctcgaaccg atcatgaacg tggaggtttc cgctccggtg gaattcgccg
     1981 gcgatctgat gggcgacatg aatgggcggc gtggccgcat ttccggtatg gaaaccaagg
     2041 gcaacacgca gatcatccgc gcgcaggtgc cgatggccga gatgctcaac taccagaatg
     2101 atttgatttc catgacgcag ggacgtgcgt cgttcacgat ggaattcgac cactacgact
     2161 tcgtccccgg catgcagtcc gaaaagattg tggcccaggc acgggcggcc caggccgggg
     2221 ttgaagaaga ggaagagtaa acctcttaag atctggcatc cgttccgggc atgtgatact
     2281 ttcaaggagc aagaaacgaa cagtctgggc tagctggttg agaggggacg gctatgggtc
     2341 gcaaacacgt ctcatggatg tttttgcttg ggactcttct gcagtttcac accgccggcg
     2401 cgcaatcggc ggggttcgaa tccctgactc cacggaagga agtgaccgaa tactgggtaa
     2461 tcgtcggcac tcccagtgaa gtctggtcta tccaggacgg tgtgatcgcc tgcaccggta
     2521 aaccatccgg ctatttgcgg agcaaacgga aatacaagaa ctacatcctg cgtgcggaat
     2581 ggcgattcga gaccgaaggc tggtcttctg cgccgccgcg ctggcctaac gcgggttttt
     2641 tcatccatgc gagcgaggaa gtaggaaaag tgtggccgaa gtcgtttgtc gaagtgcaag
     2701 gccactatgg tgaggcggga agtctttttt ccggcgggat caagggggcg aagcgcgggc
     2761 cgattgtgaa ggaccgtgtt ccgttcggcg catgggaccg gtacgagata acgtccaggg
     2821 acggcaccgt aaaggttgtg ctcaatgatg tcctggtaaa cgaaggctgg ggggtggacc
     2881 ctcccgaagg ctacatctgt ctgcaatcgg aaggttggcc ggtgttctac cggaatattg
     2941 agatcaaggt tctgcccgac tgagatgcgg ccggcagcga gagggacaca tgggaaatta
     3001 tctcgacagg cgaaggcttc tactgagcgg agcggcagtg gcggcttcct cgtccatgac
     3061 tgctcaggat caggagatcc gcgcggcatt tatcggcgta ggcgttcgcg gcggaggtct
     3121 gctgaagcaa gctattggac aacaagccgt ccgaatctcc gcaatctgcg acatcgacgg
     3181 gcaaactcgc gatcaggcgc tgagcttgag tgcccgcgat aaccccaagt cgttgacgga
     3241 atggcgtgcc gtgctggacc tcaaagacgt ggatgccgtt ttcatcggca cgccgtgcga
     3301 tttacatgcc gagatggcgg ccgcatgcct cgaagctggc aagtacgtgt attgcgagaa
     3361 gccgcttgcg gttaccgccg agggagtcga ccgggtactg cgtgccgcgc ggcgatccaa
     3421 gacttttctc cagatcggcc agcaattgcg ttattttccg tcgatgcggg aagcgattcg
     3481 ccagatccac gaaggcatcg ccggcaagac ttatatcatc aaggcgcatc gccacggggg
     3541 acgcagccgt ccgcgcggcg gcgaagccgg gccgacaccg cggcaggcga ctcgcactgc
     3601 ctggtacgcg gacgtgaagc gctccggtga cttgatcgtc gaaaacgcgg ttcataacat
     3661 cgacgcatgc aattggatcg caaacagccg gcccgttagt gcgtatgggc acggcaaaaa
     3721 gtactttccc cagccgattt cgccgggtaa ggtgatgatg gacggtttca gtgtggaata
     3781 catctatgag aacgacatgc actgcgacta cagccagatc agctttcacc cccgtcatct
     3841 gaaggcactg ccaagcggaa tgtggtacac cgtgttcgga gaaaagggca gcgtgtacct
     3901 gacacactct tcggcaactt attacgacct attcgccgag aacgaaccgc gcaaccttct
     3961 acctgagaac accagcgaaa gcgacacggc aaccgccgat ttcttcgcct gcattcggga
     4021 gaagcgccaa ccgtttgccg acgttcgagt gggcgcggtg gcggcgctga ccgccatcat
     4081 ggggcgtgaa gccatctaca agggccgaag cgtttcgtgg aaggacctgg gcgtcgaagt
     4141 atagacggat tctcacgccg gtgcccggtt gggagcaaac aatgaagaca ctgtttatcg
     4201 cgctcgcgat cctcgtgccc gcttgtgtgc ccgttccggc ccaggcccag cggccggcgg
     4261 agcgcggcct ggggcccgga tgggtgccgt tgttcaatgg gaaagatttg accggttgga
     4321 aagtggtggg gcctgaacgc tggatcgtcg aggacggcac aatcctcggc gaaggcatcc
     4381 acggcaaaga tggatttcta aagactgaaa aaacctataa ggattttcac gcctatcttc
     4441 gattcaagtg cgagacgccg aataacagcg gctttttcta tcactctgac attgaggagg
     4501 acatcagcaa aattcgattt atccaggtgg aaatatcgag tagcatcggc ggccatactg
     4561 gtggcctgca tggcgatcac actcccgaaa ccaagggacg cggctggatc gtctggccgg
     4621 caccggaact cgaaaccgtg atccggccgt tcgactggaa cgagatgttg gtcatggtgg
     4681 agggaaaacg cacccgcacc tacttgaacg gcgtaccaat gattgacttc actttccccg
     4741 atccgcgaaa tacggacggc attattgctc tacagatcca tgcgggcaag ccttcgcgga
     4801 ttcgcttcaa agatatctgg attcgagatc tgacggtacg ctgagtccag ggagtgctgt
     4861 ctgattccga ccgctcgctc acgctcgcgg ctctgtcaca agtcctgcat tatcaacaac
     4921 gcttttctga gccacgaccg tgagggagtg gtctaaccgg tcacgggctg tatcgcatcc
     4981 cgtaaacggc cgctttgcca agcgactcac ccaaaaccag caatatcaga tcgtgctttg
     5041 ggtcgtatac cattgcccgg ttctgcccgg cggctccgcg cggcgccctg cccgcgaaag
     5101 aggccgcgac cctgcgccac ctgttctcgt ccggcgaata ggcccacagc gccagaacgc
     5161 tgcggtcttc gggagccggc gaactgatga gcaggacatc cgccgctgga agatagacgg
     5221 cttcacgact ggctgcgggg ctgcctcctg ccggttccaa atgaatccag cgccgcgtgc
     5281 ggaaatcgaa cgcccacaac tcattccgcc gggctccccc gccgtgcaac atcaggcggt
     5341 cgcgcttggt gtcataggca aggctggtca actcataaag gttttgtggc gatggctgct
     5401 cgcttcccag gcgggtccag cgtttcccgg tcaggttgaa caggtacaca gtgttatcca
     5461 ccggcgggtc ctgggggctc catggacgtt ggtagccggc atcgttcaac cgtgaccgcc
     5521 aattgacgtt gacccccatg acaccgtggg gcgtgctgac cagcgtgtcc actcccagcg
     5581 gagcgggtcc tgcgagctcc catctgccgg attgggtatc gtagctgaac gttgcctggc
     5641 gtgtgtagga ggacggagtc tgcacgaccg cgtcgggtgc gacagtccgc ttcgccgggt
     5701 agtctcgtaa cgcttccggg tcgtatccgg ttgtcaggcg gatcgcgatg accatgatca
     5761 ttttcccgct gacaggatcg tgggcgtaga tgctccgtcc gtgttccgtc catggagatc
     5821 cgcggaaagt gacaccggcg gagcgcactc ccaggtccca ggcccgctcg ggaaactcag
     5881 gagcatcatc ggcgaaacgc catcggtttg tttcgggact atatgcatcc acgtcgctgc
     5941 cgccataacc gcagtggcct ccaccccagt agaggatttc gccgcgcgcg gtatcgaatg
     6001 tagccgagcc ccaggtacgg accggcgcgc tttcctgagt gttgttgagc aatacccact
     6061 ggttcaacgg tagcgagtcg aggcgggcac tcgcggatgg cttattcgcg ttcacccgct
     6121 tcgcgatcgg ctcatgccgc accgcagcag gcttgacagc ggccctgagg gccttggaat
     6181 cgatccggaa cccgtaggtc gtacgaaccg gataaagcac gttgcaggtc atccggtcat
     6241 ccggcctgcg gttattcgtc agcagcacaa tcaaccgctt gtccgaagca atatcggcgg
     6301 tgatgtagaa acctgccgga actttggcgt ccagttccca ccaccggtcg gcggccgcat
     6361 cgtatgccca catgtccgtc aggtcgcgtt ggttgtagcc gccgccaatg atgacccagc
     6421 ccgtttcggg atcgtatacg gtgaagtgtc ccgcgcggcc ttggggtccc gcgggtgcct
     6481 tggatactcg ccatgaccgc gttttcatgt catacagcca cgtatccgcc aggaaatgac
     6541 tctgaccgtc gccgccgaac agcacgagca cgccattgcg cgtgtccgcc accattcgtg
     6601 tattcatgcg tggcggcggc tgcacacccg caggcagcag gagccagtcg cggtcccgaa
     6661 gaacccaggt tccggtgtag ccggtaaggt tgccgctccg gtcgctttcc gcgacatggc
     6721 cgcctccgaa cagaaccatt tcgccgttga ctgaatcata cgccagcgag ccgcccacta
     6781 cggggggtgg agagtggagc ggacgcaagt ccgtccagcg cctcccggcc gtatcgtagg
     6841 cggccgtcag cccgcccgta aagtacagga gcgacttcat ccgcggatgc caggcaacct
     6901 gatcgaagac gatgttcaga tcgggacgag gcacacccgg ggcatcattt gtggcaccgc
     6961 gcagcaccgt gcgctcactc ccgctggtga tcgctgagta cgtccgtgga acataggcca
     7021 gcggcagctt ccggctccac tcccgctcgc gcgacttcgg cagttgattc tgccagcggc
     7081 caataccagg gtcgaatgcg acgacgtcgt attcagggac ttccatcaat ggatgttcct
     7141 gaaggaattc gggatcgtga tcgaagaatc cccacaaaaa gaaggcgccg gcatcgggcg
     7201 cataacgaac cgcgctgccc cggcgtcctc cggagggatc ccggaccagt tccacccatt
     7261 gattcggcgg agtgtcggcg gccatggcaa tcatggcaga cacaagtaaa gaaacaaggc
     7321 gcagccacat aacgattgat gatacgacgc tgttacttaa tctcacgcat agcttccagt
     7381 tccgcccgca ctcgagcgat ggtgtcgcca tattcggcag gagcggcctt aacaaggaac
     7441 gtctcgaaac attcgcgggc gcgagtgttg gcgcccgtca tcctgtagat ccgccccagg
     7501 ttgagatagg cctccagaag attcgggtca aaatggacag ctttctcgaa gtgaccgcgt
     7561 gccgcgatca tgtcttgtct ccgaacagcg acgagaccaa gaccgttata cgtcagagca
     7621 tactcttccc ccgaagccag acaccattgg aaaacgcgtt cggcatccct ggtcctctcc
     7681 atgtccaaat aaatcaatcc gagatcgctc aggctttcgc aatccgtggg gttcaactgc
     7741 gccgctcttt cataagccac aaccgcctct tcccggcgcc cctgctggag gtacaagtga
     7801 ccaaggcggg cgtagatcca ggcagtacga acaccattcc gaatcccgtc ctggtagagt
     7861 tgcatggcct ctttcagacg tttcgccctc tggtattcat ctcccaggtg caggtagacc
     7921 gtcgggttga ccggatcttt tgccagcgcc tggcgcaacc agccaagccg cagggccgcg
     7981 ggggccgggg catccggcga aaccgccagg tgaagcaact tcatcagctc gagccggtcc
     8041 ttcggatcga ctccttcgcc cgtcagccga tactcctgct gagccatacc cgccgaataa
     8101 cccagggact tcaattgctc catggtgcgt tggtcaacca ccaccgttcc tacgatttcc
     8161 ggatttctgt tgccggaagg tccggcgaac tccgccagcc tggcctccat ctctcgcact
     8221 tcgttggggt gatccgcgat gacgttcctg gtttctgccg gatcttgcac caggtcatac
     8281 aactccggct ttggcgcgcg gatatatttc cattggttcg tacggatgcc tctcagttcc
     8341 gtccatccca tgttgatctt agggaacaga gtctcggcat aagagtaagt cgcgggcagt
     8401 tgcttcccca tgagagtggg agcgaggctg atgccttgca cgccggccgg agcctgacta
     8461 cccatcagat cgagaattgt cggcagtatg tcgatgcttc tcgcctgctg cttcagccgt
     8521 tgacccggcg gaataccggg gccggcaatg atgaatacga gccgcaatgt cgaatcgtag
     8581 aggaagactc cgtgtgtgta ctccccatgt tccgacaggc tctctccatg gtccgataag
     8641 actacgatca gagtatcttc cggtgacgac tttcgcccta cggcatcgaa cagccgtccc
     8701 aattgctgat ccgtgtaagc tacctcaccg tcataacgcc ggcccgcgta tttctcccga
     8761 aaaggagatg gcggatcgta cggcgagtgc ggatcgaaaa catgcaccca gatgaagaaa
     8821 ggcttccccg attgcgaatt gagccatcca agtgcgcgat cgactacctc tccggcgcgg
     8881 cgttctggaa gctcactccc catgccgctt gcagccggtt tcggcatctg gtcgtcgtag
     8941 actctgaacc cgcgatggaa gccgaagcct ttcttcagca cggaggctcc gacaaatgcg
     9001 gcagtatccc acccccggct ttgcagggtc tcggcgagaa ccgggctcga ggatgggaga
     9061 acgaatcctc cggtatcccg aactttgtgg actgtcggat tttggccggt aaatatactc
     9121 gcgtgagacg gagcggtcag cggagtctgg gcgatagcgt tctcgaatag agcccctttc
     9181 ctggccagac tgtccagatt tggcgtctgg ttatccggat ggccgtagca gccgagcctg
     9241 tcaggacgaa gggtgtcgat agtcaccaga acgacgttga gttgccgaac ggtcttttgc
     9301 tccggcggcc ggcacgaaag ggaggtgaat accgccgccg cgcacagcag tacccccaac
     9361 ttgctcatgt ccaccgcccg tcgagcgcag tacgcgcggc cggtcgaacc taccatgtca
     9421 gccgtagagt gagttgcaat tcacgtgccg gctgaccgct gttaaatgtg ttcagtactc
     9481 cgttacttcc cacgctgtta ggattaccag gggcattcag cacgttgaag aaatctgcat
     9541 tgaatcggac cctgacttgc tctccgaatg caatcgtctt gaacagcgaa gcgtccaagc
     9601 cccactgccg gacgctgggc agatactggt tccgccatgg atgcaggttg tcgttgaagg
     9661 ttgtgcgctg caccgtgccg ttttcgagcg gaatccagac cgtgttcgtg tcccagaagg
     9721 aagcgacatt ggtattcgcc ggcgcattgg gtggcagcac cgtagagccc catggaatca
     9781 acggctgtgc cgccggtttg taattgtccg gcacgcccat aataccgtta ggctttccgg
     9841 atgcatcgtg gctgttgatc tggcttgcgg gaatgtatcc gttccaccaa aggtagcctg
     9901 gccggcaggc gccgctgcgg caatcctcga tgggatactg gtagccataa agctcgacct
     9961 tgtccccagt cggataaact cctgtcggca atgcgaagta agtgctgcgc agtgagccca
    10021 tgccggcgat ctgccatccg ccgataagtt tgtccagcat gccgctggaa ttgcgtccca
    10081 acgctttgcc ccggccgaag ggcaggtccg caatccaatt ccatcgaagc cggtgttttg
    10141 gaacatccgc gtcccggcgg tagtgcagca aacgtgcgcg ctcgtgaaaa tccgccggca
    10201 ccgcacccgg aagatacgag ttcagttcgg gaacactgat tcgccactgc tcaccacccg
    10261 cttcgagagc gttcccgacg ttgtagaaca gttggaagcc gtagccgccg ctgtagcggc
    10321 gttcgacttc gagcgtgaca ccgctgaaat tgccccaacc gtccttgcgg tattcctgta
    10381 ccgtgccgta taccgtctgg tcataaaagc ggcgggccac gttggcgtac tcgccggtgg
    10441 gcagcaactg tctttgggtc gcgtaccaga tgtactcggg agtcgcgcca ttgtaatcgt
    10501 agagcagctc gagattgctg gtgtgattgc cgacatacga cgcacgcacg acggtgttcg
    10561 gtatgacttc cttttccagg gtcaggttcc agtcgtggac tcgcgtatcc ggctggttct
    10621 gtgcaaaata gcttgcaaat ggcgatcccc gaacgattga actcacgttc gacaggctga
    10681 tcgcgtcccc gctgttcaca cccgcgacga tggtcggcgc cgacctcata tagtagttgg
    10741 caatcccatc cggcgaaaga gcggagtcgt ttatgttgct ggtcaaacgc gcataatagg
    10801 gcgcattcga gcgctgttgc gcggtccagg cacggagcgg aatcgtaaaa tgtgagatcc
    10861 ggtagccgcc gcggagcacc atcggcctgt cattacttcc gaaccggtat gcgaatcctg
    10921 cccgcggacc gaaattcgtt ttcgggaccc tgtaaagaga cttcggaagt ccggcttctt
    10981 cataaccgat gaacttcacg cccagcgatt gcattctctc gaccactgaa gggagagtcg
    11041 ctccaagacg gtacatcgtc tccagatcgg agcccagtac gatcgccctc tgtgtgggat
    11101 cgaacgaagt caacaggttg ctgacctcac ggttggccgg ccagtaatcg tagcgcaagc
    11161 cgaggttcag agtcagccgt gagttcacct tgaaattgtc ctggaagtag agggcatact
    11221 cctgcgcccg catcttaaac aacggatgac cgagctgcac gtagtagttc atcaatccca
    11281 gataactgct ggccaggttg tagccggtct gctgcgtcgg gagcgggtta ttgcgggaag
    11341 tcgtcgggtc gtatagagaa gtgaaaatgc tggagccatt cgcctggcct tgcgtgtatt
    11401 gttgctccgg aagcgtatcc agcttgtccc atcgatagtg gaaaccaaac tggaattcgt
    11461 gcctgcctac aattttcgtg gcgttatcgt caatgatgta gaaggcgaac ggagttgtct
    11521 gcgtgttctc cgtttcaaag tccatgtcca ggccgtgact atagagagcg ggccagccgg
    11581 ccacgttgtt aggattcgga atccccaact ggtcggacca tcgggtattc gggtcgccgg
    11641 tacccgaac
//
LOCUS       DASTMK010000281         7098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_429432, whole genome shotgun sequence.
ACCESSION   DASTMK010000281 DASTMK010000000
VERSION     DASTMK010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429432"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFQ79_12140"
     CDS             complement(<1..210)
                     /locus_tag="VFQ79_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140462.1"
                     /translation="MSLRARVYAVLALILVLGPGIYLLNQKFSFFPWMAAASERGKAK
                     SDNKESGEKKGTSDASPVELTMAKQG"
     gene            complement(343..735)
                     /locus_tag="VFQ79_12145"
     CDS             complement(343..735)
                     /locus_tag="VFQ79_12145"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0140463.1"
                     /translation="MKRTFSAIALILICFLPLAAQSKTAKHSRQGGAAAVAPDAQIEA
                     SIRQRFARSKISTNNFTVRVQGGVATIEGRTNVVQHKGTATRLAKAAGARRVVNNIKI
                     SDEARAKAAGNLEKNRRRVQVRRSEVTR"
     gene            complement(797..1945)
                     /locus_tag="VFQ79_12150"
     CDS             complement(797..1945)
                     /locus_tag="VFQ79_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0140464.1"
                     /translation="MGFETSSKIEQQAIWSRYRAEFPVTGHLTYLNHAAVAPLCRAAA
                     EAMHELADDALLYGSYHYDEWLDAYEGVRRAAAQLLGAEREEIAIVKNTSEGIATVAI
                     GLDWRAGDKVVAFEEEFPANYFPWKRLESRGVRVEWLSATDPLDRINEATRGARLLAI
                     SFVQYLSGHRADLQAIGEICHRQGATFFVDAIQGLGAFPLDVNACHIDALAADGHKWL
                     LGPEGCGILYIRRELQDRVDPVEFGWTNVARYADYASRDMSLRPDAGRYECGTLNTIG
                     CFGLRAAMEFLLEVGVERIAPHVQALGDRIAEGARRKGCEVLGVRTPANGAGIVSFRH
                     PEVDARAIVHRLKERGIIAAPRQGWVRTSPHFYISPDDVDRMLEVLPG"
     gene            2042..3031
                     /locus_tag="VFQ79_12155"
     CDS             2042..3031
                     /locus_tag="VFQ79_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU0140465.1"
                     /translation="MQRAFSFHLGLALALSLLVPNAGFAKDKKKDPEEIGNRDVGKGV
                     NFYSLEKEIGLGKQLAQEIERQAKIVDDPVIAEYVNRIGQNLVRNSDAQVPFTIKVID
                     GEEVNAFALPGGFFFVNSGLILRAESEAELAGVMAHEIAHVAARHGTRQATRGQLVNL
                     ATIPLIFMGGWTGYGVRQGASVLIPIGFLRFSRGFEEEADLLGLQYLYKAGYDPTAFV
                     DFFEKIQSDEKRKPGTMAKVFSTHPMTEDRIKNSQQNIAEILAAKPEYVVSTSEFENV
                     KARLSALQNRRKIDDKDDKSRPTLRRTPGSGPIGDDEEKQKPDEDERPTLKRR"
     gene            complement(3098..3580)
                     /gene="moaC"
                     /locus_tag="VFQ79_12160"
     CDS             complement(3098..3580)
                     /gene="moaC"
                     /locus_tag="VFQ79_12160"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU0140466.1"
                     /translation="MSSDLSHFDEAGSARMVDVSAKQNTRRTARAHAFIRIRADVLKK
                     LPENPKGDPLEVARLAGIMAAKKTAELIPLCHPLLLSHVDVDIGVEEAGVRIVASATT
                     TGPTGVEMEAMTAAAIAALTVYDMTKALDKAIEITDLRLLEKTGGKSGEFHRLPDTGA
                     "
     gene            complement(3577..4770)
                     /gene="glp"
                     /locus_tag="VFQ79_12165"
     CDS             complement(3577..4770)
                     /gene="glp"
                     /locus_tag="VFQ79_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU0140467.1"
                     /translation="MATLSFEDARQCVFERIRGAIGDIPSQDASLADVQGRVLAEDIC
                     ADRDYPPLARSVRDGFAIRSQDLPGEVTVIGEVAAGDVSAVAVGSGQAIEIMTGAPLP
                     AGADAVLMVEHAVRDGTTIRTGRTVRSGEFVNFQGSEAKAGDVLAAKGVRAGFAEVAL
                     LASTGHVRVRVYKRPRVAILATGNEIVEVKEPVLPQQIRNSNAYSLAAQVRRAGGVPS
                     ILPVAKDQLDMTANLVGEGLDYDLLLISGGVSAGKYDVVEQALARHDAEFYFDRVRIQ
                     PGQPLVFGRARGTFFFGLPGNPASTMVTFEVFARAALDLLGGQTESPLPLTLARLTRE
                     FTHKPGLTRFLPAHLSDGTAEITPIPWQGSSDVPALSRANAFLVADAERESWKAGDLI
                     QVFLK"
     gene            4810..5943
                     /locus_tag="VFQ79_12170"
     CDS             4810..5943
                     /locus_tag="VFQ79_12170"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU0140468.1"
                     /translation="MLKLTWKQAAAWRVRSHHLDRRAPAGSMLAVASRLCGLHAQVMS
                     SAELTVWARVEDLDRQAVQRALWEDRTLVKTWAMRGTLHLLPAGELPLWHAALSTSRR
                     YLKPALWQKYFGITIEELDRLTEAIAAALDGRLMTRGELVEEVARLTGSPELGVKLAE
                     SSWGTILKPAAFTGRLCFGPSLGQRVRFTHPDTWLAKTLPPVDSQTAMAGVTRRFLSA
                     YGPATHHDLARWWNGGGVSTARQWIAALGDEVSAVDLEGDRAWMLAADAREIRELPPN
                     RSVRLLPAFDQYILGASCHAQHLLPGDLRHRVYRPQGWISPVLLVNGRMEGVWRHEIR
                     SSRLDVTIEPFVKTPLWVRRAAGQEAERLAAFFGCSLNLTWKI"
     gene            6007..>7098
                     /locus_tag="VFQ79_12175"
     CDS             6007..>7098
                     /locus_tag="VFQ79_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140469.1"
                     /translation="MRALCCVFFALILSAQVPAPVPQPQQPQWLMDLVKIRQELHQAE
                     LGSLDAKVEQAWRAALATPQHPRFPEAAQMAWGCYQAQGYDLKAEQVLRQALAAVPTD
                     DPRIRRNLRSNLAIHFESTQQLVKALSIREEMAKEPPPATDGYSHETAALANLYERMG
                     EIEKAEAAWKEAAASRAAGSPQNDSRFARTARRAFSPFGYVRGSQNELAGFYARRGRT
                     AEAEQLYQKALDDAAQNNSAYEWSRAADNYIGFLSQERRFSEAIDLVRQSIARLEASA
                     DPQDARMLLSKRQHLASLLTQAGRSDEALEMQKQTVELAQAQNPSGLEYVQALGSLAE
                     TLVSQNRLEEAEKSVARMREAGAADTRNGK"
BASE COUNT         1403 a         2247 c         2113 g         1335 t
ORIGIN      
        1 cccctgtttc gccatcgtca gctctaccgg actggcatcg gacgtcccct ttttctcgcc
       61 cgattccttg ttgtctgatt ttgctttgcc gcgttcggat gccgcagcca tccaggggaa
      121 aaagctgaac ttctggttca gcaggtagat gcccggaccc agaacgagta tcaaggccag
      181 gaccgcgtaa actctcgcac gaaggctcat aacgcgaatc tcccgggaca atgcggtagg
      241 tgcgccgcaa ttcgaacagg tatccagttg ctatctgcga tagacaccag caagacgaaa
      301 aagttaggcc gggagttccg tcgatttttg gatgttcgag cgttaacggg tgacttcgct
      361 tcgccgcacc tgcaccctgc gccggttctt ctccagattc ccggcagcct tggcgcgtgc
      421 ctcgtcgctg atctttatat tattgacgac acggcgggcg cccgccgctt tggccaggcg
      481 ggtagcggtg cctttatgct ggacgacatt cgtgcgcccc tcgatggtgg ccaccccgcc
      541 ctgcacccgc accgtaaagt tgttggtgga aattttggag cgcgcgaacc gctggcggat
      601 ggaagcctcg atctgcgcat ccggagccac tgctgccgcg ccgccctgcc gggaatgttt
      661 cgctgtcttg ctctgagccg ccaggggaag aaagcagata agaatcagcg cgatcgcgga
      721 aaaggttcgc ttcaaagcgg ggtcctccaa caactagtgc ccggaagcgg gaaaggttct
      781 cccggaagcc gttgccttag ccgggcaaca cttccagcat gcgatccacg tcatcggggc
      841 tgatgtaaaa atgcggggac gtgcgcaccc agccctgccg cggcgccgca atgatcccac
      901 gctctttcag gcggtggacg atcgcgcgcg cgtccacttc gggatggcgg aagctcacga
      961 ttcccgctcc gttggcggga gtccgcactc cgagtacttc gcatcctttt cgcctggcgc
     1021 cctcggcgat gcggtccccc aatgcctgta cgtgcggggc aatccgctcg acgcctactt
     1081 cgagcagaaa ctccattgca gcccgaagcc cgaagcagcc gatggtgttc agcgtcccac
     1141 actcatagcg gcccgcgtcc ggccggagcg acatgtcacg ggaagcgtag tcggcatagc
     1201 gggcaacgtt cgtccatccg aactccaccg gatcgacgcg atcctgtaac tcgcggcgga
     1261 tgtaaagaat tccgcaaccc tcgggcccaa gcagccactt gtggccatcc gccgctaagg
     1321 catcgatgtg acaggcattt acgtccagcg ggaaggcgcc caagccctga atggcgtcca
     1381 cgaagaaagt ggccccctgg cgatggcaga tctcgccaat ggcctggaga tccgcccggt
     1441 ggccgctaag atactggacg aagctgatgg ccaggagccg cgcgccgcgc gtggcctcat
     1501 taatccggtc cagcggatca gtcgcggaaa gccactccac gcgcacaccc cggctctcca
     1561 gccgcttcca gggaaagtag ttagcgggaa actcctcctc gaacgccacc actttgtccc
     1621 ccgcgcgcca atccaaaccg atggccacgg tggcgatgcc ttccgaggta ttttttacaa
     1681 ttgcaatctc ttcgcgctcc gccccaagga gctgtgccgc ggctctccgg acgccttcgt
     1741 aggcgtccag ccactcgtca tagtggtaag agccgtaaag caacgcgtcg tcggccagtt
     1801 cgtgcatggc ttcggccgcg gcgcggcaaa gcggcgcgac cgcggcgtgg ttcaggtaag
     1861 tcaggtgccc ggtgaccgga aattcagccc ggtaacggct ccagatcgcc tgctgttcaa
     1921 tttttgagct ggtttcgaaa cccattttcc ccaaaatgcg ttaactaaat taaggctgcc
     1981 tgtgatccat cctgtatggt ggcctataat agcaggtaga ggttccgggg aaggagttcc
     2041 catgcagcgt gctttttcgt ttcaccttgg cttggcgctc gccctgagct tgctggttcc
     2101 gaacgccggc tttgccaaag acaagaaaaa agatccggaa gaaatcggga atcgcgatgt
     2161 tggcaaaggt gtcaacttct attctctcga aaaagagatc gggcttggca agcagctcgc
     2221 ccaggaaatc gagcggcagg ccaagattgt tgacgatcct gtcatcgccg agtacgtaaa
     2281 ccgcatcggc cagaacctgg tccgcaattc agatgcacag gttcccttca ccatcaaggt
     2341 gatcgatggc gaagaggtga atgcgtttgc gcttcccggt ggatttttct tcgtcaactc
     2401 gggattgatc ctgcgcgcgg agtcggaagc tgaactggcc ggcgtgatgg ctcatgaaat
     2461 cgctcatgtg gccgctcgcc atggcacccg tcaggcaacc cgcggccaat tggtgaatct
     2521 ggctaccatc ccgctgatct tcatgggcgg ttggacggga tacggtgtcc gccagggcgc
     2581 cagcgtgctg atcccgatcg gctttttgag gttttcgcgc ggctttgaag aagaggcgga
     2641 cctgctgggc ctccagtacc tttacaaagc cggctatgac cccacggctt tcgtggattt
     2701 cttcgagaaa attcaatccg atgaaaagcg aaagcccggc accatggcaa aggtgttttc
     2761 cacgcacccc atgaccgagg accggatcaa gaactcccag cagaacatcg cggagattct
     2821 cgccgccaaa cctgagtacg tggtgagtac ttcggagttt gagaacgtca aagcacggct
     2881 atcggcatta caaaaccggc ggaagatcga cgataaggac gacaagagcc gtcccacgct
     2941 gcgccggact cccggcagcg gccccatcgg cgacgatgaa gagaaacaga agcccgatga
     3001 ggatgagcgc ccgactttaa agcgccggta agttttctct ccccgcaagc tgcatcaggc
     3061 ccacgcccga atctcaaggg tgggggcctt gttgcattca ggcccctgta tccggcaggc
     3121 ggtgaaactc gccgctcttt cccccggttt tctcgagcag ccggagatcg gttatctcga
     3181 tggccttgtc cagcgccttg gtcatgtcgt acaccgtcag cgcggcaatc gcagccgccg
     3241 tcatcgcctc catttccact ccggtcggtc ccgttgtagt agcgctggct acaatccgta
     3301 cgcctgcctc ctccacaccg atatccacat cgacgtgcga caacagcagc ggatgacaca
     3361 gcgggatcag ttccgcggtc ttcttggcag ccataatgcc agccaggcgc gctacctcca
     3421 atggatcgcc cttcgggttc tccggcagtt tcttcagaac gtccgcgcga atgcgtatga
     3481 atgcatgcgc ccgtgcggta cgcctggtgt tctgtttggc agaaacatcc accatgcggg
     3541 cgctgcccgc ctcatcaaaa tgcgataaat cggaactcat ttcagaaaca cctgaatcag
     3601 gtcaccggct ttccacgatt cccgctccgc atcagccacc agaaacgcgt tcgcccggga
     3661 gagcgccgga acgtcgctcg atccctgcca ggggatcggt gttatttctg cggttccgtc
     3721 ggagaggtga gcgggaagga agcgggtcag cccgggttta tgggtaaatt cccgcgtgag
     3781 ccgcgccagg gtcaacggca gcggactctc cgtttgtccg cccagcagat ccagcgccgc
     3841 gcgcgcgaag acttcgaacg tcaccatcgt ggaagcgggg ttgcctggca gtccgaaaaa
     3901 aaacgtgccg cgcgcgcgcc cgaacaccag cggctggcca ggctgaatac ggacgcggtc
     3961 gaaatagaac tccgcatcat gccgggccag ggcctgctcc acaacgtcgt atttccccgc
     4021 ggacacgccg ccggatatca gcagcaggtc gtagtcgagg ccctccccaa ccaggttggc
     4081 ggtcatgtcc aattgatctt tcgccaccgg aagaatggag ggaaccccgc cggcgcgccg
     4141 cacctgggcc gccagcgaat aggcgttgga gttgcggatt tgttgcggta gtacgggctc
     4201 cttcacttcc acaatttcat tgccggtcgc cagaatcgcc actcgcggcc gtttgtacac
     4261 gcggacgcgc acgtgccccg tcgacgccaa caaggcaacc tcggcgaacc cggcgcgaac
     4321 ccccttggcg gcaagcacat ctccggcctt agcctccgag ccctgaaaat tgacgaactc
     4381 gccggaacga accgtgcgcc cggtgcggat tgtcgtcccg tcacgtaccg catgttccac
     4441 catgagaacc gcgtccgccc cggcaggcag cggggctccg gtcatgatct caatcgcttg
     4501 cccgcttccc acggcgaccg cggagacatc ccccgcggcc acttctccaa tcaccgtcac
     4561 ctcgcccggc aggtcctgcg aacggatagc gaagccgtcc cgcacggaac gcgccagagg
     4621 gggatagtcg cggtcggcgc agatgtcctc ggccagcacg cggccttgca catctgccag
     4681 gctggcgtcc tgcgaaggaa tatcaccgat tgccccgcga attcgctcga atacgcactg
     4741 gcgggcatcc tcaaagctca aagtggccac aaacgcattc tacctgccgg ttggcataat
     4801 gattagcgga tgctgaaact cacctggaaa caggcggcgg cgtggcgcgt tcggagtcac
     4861 catctcgacc ggcgcgcgcc cgccggcagc atgctcgccg tcgccagccg cctctgcggt
     4921 ttgcacgcgc aggtgatgtc ctccgccgag ctaaccgtat gggccagggt cgaagacctc
     4981 gaccggcagg ccgttcaacg cgctctgtgg gaagaccgga cgctcgtcaa aacctgggcc
     5041 atgcggggca cgctgcacct gcttcccgcc ggcgagttgc ccctgtggca cgccgccctc
     5101 agcacgagcc gccggtatct gaaacctgcc ttgtggcaga agtatttcgg gatcacgatc
     5161 gaagagctcg accggctgac ggaagctatt gccgccgcgc tggacggccg cttgatgaca
     5221 cggggcgagc tggtggagga ggtggcgcgt ctcaccgggt cgccggaact cggcgtcaag
     5281 ctcgcggaaa gcagttgggg aacaattcta aaaccggccg ccttcaccgg ccgtttgtgc
     5341 ttcggcccta gcctggggca gcgcgtccgg ttcacgcatc ccgatacctg gctggcgaag
     5401 acgctgcctc ccgtcgattc gcagaccgcg atggccggtg ttactcgccg ctttttgtcg
     5461 gcctacggtc cggcaacgca tcatgacctc gcacgctggt ggaacggcgg cggcgtttcc
     5521 accgcccggc agtggattgc cgctctcggc gacgaggtgt ccgcggtcga cctcgaaggc
     5581 gaccgggcct ggatgctggc ggctgacgcg cgcgaaatcc gcgagcttcc acccaaccgg
     5641 tccgtccgcc tgctgcccgc cttcgatcag tacatcctcg gcgcatcctg tcacgcgcag
     5701 cacctgctgc ccggcgactt gcggcaccgc gtctaccggc cgcaaggttg gatctccccg
     5761 gttctgttgg tcaatggccg catggagggc gtctggcggc acgaaatcag aagcagccgc
     5821 ctggacgtga ccatcgaacc gttcgtgaag acaccgctat gggtgcgccg ggcagccggg
     5881 caggaagctg agcgcctggc cgcgttcttt ggctgctctt tgaacctcac ctggaagatc
     5941 tgaaagcgcc tcttgcatcc gttttttccg ggaaccggat cagcgtgtgg cgatgtctaa
     6001 atggacatgc gcgcactttg ttgcgttttc ttcgcattga ttctctccgc tcaagttccc
     6061 gcgccagtac cgcagccgca gcagccgcaa tggctgatgg acctcgtgaa gatccggcag
     6121 gagctgcacc aggccgaact cggctctctt gatgcgaaag tcgagcaggc atggcgagcc
     6181 gcgctcgcta caccgcaaca tccccgattt ccggaagcgg cacaaatggc gtggggctgc
     6241 tatcaagcac aaggctacga cctgaaggcg gaacaggtgc tgcggcaggc tctcgcggca
     6301 gtgccgacag acgatccacg gatacggcgg aatctgagat ctaacctcgc catccatttt
     6361 gagagtacgc aacaactggt gaaagcgtta tcgattcggg aggaaatggc aaaggagccg
     6421 ccgcccgcaa cggacggata cagtcacgaa accgccgccc ttgccaatct ttatgagcga
     6481 atgggtgaga ttgagaaggc tgaagcggca tggaaagaag cggccgcatc gcgcgcggcc
     6541 gggagcccgc agaacgattc ccgtttcgcc cgaacagcga ggagagcatt ctctccgttc
     6601 ggttatgtca gagggagtca gaacgagctt gccggtttct acgcgcgccg tggacggacc
     6661 gccgaagcgg agcaactcta ccaaaaagct ctcgacgacg cggcccaaaa caattccgcc
     6721 tatgaatgga gccgggctgc ggataactac atcggattcc tctcgcagga acgccggttt
     6781 agcgaagcca ttgatctggt ccgccagagc attgcgcggc ttgaagcctc cgcggacccg
     6841 caggatgcca ggatgctcct ctccaagcgc cagcacctgg cctctcttct aacacaggcg
     6901 gggcgttccg acgaggctct tgagatgcaa aagcagaccg tcgagttggc acaggctcaa
     6961 aacccgtccg gtctcgaata tgtccaggcg ttaggatcgt tggccgaaac gctcgtcagc
     7021 cagaatcgtt tggaggaagc cgagaagtca gtggcccgga tgcgtgaagc gggagccgcc
     7081 gatacacgca atgggaaa
//
LOCUS       DASTMK010000282         3873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_301956, whole genome shotgun sequence.
ACCESSION   DASTMK010000282 DASTMK010000000
VERSION     DASTMK010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301956"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFQ79_12180"
     CDS             <1..842
                     /locus_tag="VFQ79_12180"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140470.1"
                     /translation="LENVCYFRNSLLILNMIRQGLFGELTHCEGGYQHDVRGAFLSRG
                     GERGPAGEITWRGMHAVRNNGNLYPTHPLGPIAWWLDINRGDRFTHLTSMSSKSRGIN
                     KYASDNYGADHPNARRQYALGDVNVTLIRSENGATVTLYHDTSSPRPYDLILRVQGTH
                     GIYMGTLDKFYFDKRSPNKEQWEDPERYYREYEHPLWRDLGAEARSHGHGGADYIEIH
                     QFLKAVRNRTQTPIDVYDTAVWSSIYPLSVRSVAAGSQPMEVPDFTRGKWKTREPVKI
                     YGA"
     gene            complement(859..933)
                     /locus_tag="VFQ79_12185"
     tRNA            complement(859..933)
                     /locus_tag="VFQ79_12185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(899..901),aa:Gly,seq:tcc)
     gene            complement(1002..1898)
                     /locus_tag="VFQ79_12190"
     CDS             complement(1002..1898)
                     /locus_tag="VFQ79_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEU0140471.1"
                     /translation="MILVVGATGLLGQEICRRLRERGMPVRALVRPTANPERLNSLRE
                     IGVELFGGDLKNIDSVSAACHDVHTVVNAASSVFSRQAGDSVGSVDWDGSLSLVRFAR
                     VLGVDRFVYITVPHELHCDCELFKAKNDVIRELKYHAMPYTILSTNYFMEVWLSNTCE
                     FDVMNGKVTIFGAGTAPVAWVSRSDVAEITVRSLDSAATLNQDLNVGGPENLSPLEVV
                     RIFEEVSGQRYSVTHIPESRLEADFRAAKDPSERAIAAFKLEYARGCPMDMPETLRRI
                     PIELTSVRTYAGRLLAPKTATA"
     gene            1993..2481
                     /locus_tag="VFQ79_12195"
     CDS             1993..2481
                     /locus_tag="VFQ79_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140472.1"
                     /translation="MYYGVGICRRLAVELLGCSPRGNYSMSTVAETNLIQKMEPLSRG
                     RTDLRKRKPKQIAIINENCTGCAGSPACVAYCPVEACMFWIPDEEYSPFGRIEVDKST
                     CIGCAKCTSKGPDGTFLDGCPWDAIEMVAIGEWEQSHGVVLPAEPDRPATEWDYVRRD
                     YV"
     gene            complement(2444..3316)
                     /locus_tag="VFQ79_12200"
     CDS             complement(2444..3316)
                     /locus_tag="VFQ79_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU0140473.1"
                     /translation="MTPNSIPPPQPAKARAPRRWLLIVMWAVAISLVSMPLIFWYQTW
                     FGRELTDEDLSRYLQDEQHPRRIQHALSQISDRMSRGGSSVSGWYPQILAAARNSRPE
                     IRSTAAWVMGQDNNSQIFHDALLKLLSDPEPSVRRNAALSLVRFGDGRGRPELLDILR
                     PQSIRAPVDGVVALDTPEGQAVVAGIAVGSITGSQGEEIPLRAPLSGRLESLAVKDGS
                     RVKRGDEVLFVRADSSEVWEALRGLYFVGSESDLEQIDQYRGELPDMDGRIRQQAALT
                     AEAIRNRAGRSPIP"
     gene            complement(3316..3582)
                     /locus_tag="VFQ79_12205"
     CDS             complement(3316..3582)
                     /locus_tag="VFQ79_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140474.1"
                     /translation="MILNLICSVIALGALGAVAWYLITGRASQDGIDGLFWIFSFLVV
                     AVLFSLMPLQSLRAALPRDLLKRVRRKPQTDREQQIAQAASQKQ"
BASE COUNT          862 a         1165 c         1105 g          741 t
ORIGIN      
        1 tcctggagaa tgtctgctat tttcgcaact ccctactcat tctcaacatg atccgccagg
       61 ggctgttcgg cgaattgacg cattgtgaag gcggctacca gcacgacgtg cgtggagctt
      121 tcctttcgcg cggcggcgag cgcggtccgg cgggcgaaat cacctggcgc ggaatgcacg
      181 ccgtgcggaa caacggcaat ctctacccga cacatccgct cggccccatc gcctggtggc
      241 tcgatatcaa ccggggcgac cggtttactc atctcacctc gatgagcagc aaatcgcggg
      301 gcatcaataa atacgcttcg gacaactacg gtgcggatca tccgaacgcc cgccgccaat
      361 acgctctcgg cgatgtcaac gtgacgctga tccgctctga gaacggagct acggtcacgc
      421 tataccacga cacttcgtcg ccacgcccgt acgacctgat cctccgcgtg cagggcactc
      481 acggcattta tatgggcacg ctggacaagt tttatttcga caagcgctct cccaacaaag
      541 agcagtggga agatccggaa cgttactacc gggagtacga gcacccgctg tggcgcgacc
      601 tgggggcgga agcccggagc cacgggcacg gcggcgccga ctatatcgag attcaccagt
      661 tcctgaaagc ggtcagaaac cgcacgcaaa cacccattga tgtttacgat acggccgttt
      721 ggagctctat ctatccgctc agcgtgcggt cggtcgcggc tggcagccag ccgatggaag
      781 taccggattt tacacggggt aagtggaaga cacgcgagcc ggtaaagatt tacggagctt
      841 aagggcgggg gaaaaccatg gagcgggaga cgggaatcga acccgcaacc aacagcttgg
      901 aaggctgtga ctcgaccatt gagttactcc cgcacgttcc ttcaacattg tacggaaccg
      961 gcgatcagcc ggtcaatgta cggcggtttt tgcacgtggg cctaggccgt tgcggtttta
     1021 ggagccagca ggcggccagc gtaggttcgg accgatgtca gctctatcgg gatccgacgt
     1081 agggtctccg gcatgtccat cgggcagcct cgtgcgtatt ccagtttgaa cgccgcgatc
     1141 gcccgctcgc tgggatcctt tgccgctcga aaatcggctt ccagacggga ctcgggtata
     1201 tgagtgaccg agtaccgttg tccggagacc tcctcgaaaa tgcgcaccac ttcaagaggc
     1261 gagagattct cgggacctcc cacattcaga tcctggttca gcgtggcggc ggaatcaagc
     1321 gagcggacag taatctccgc cacatcgctg cgggaaaccc aggcgactgg cgcggttccg
     1381 gcgccaaaaa ttgtaacctt accgttcatg acgtcgaact cacaagtgtt gctgagccaa
     1441 acctccatga agtagtttgt cgagaggatg gtgtaaggca tggcgtgata cttcaattcg
     1501 cgaatcacgt cgtttttcgc cttgaaaagc tcgcaatcgc agtgcaactc gtgcggcacc
     1561 gtgatgtaaa caaatcgatc gacgccaaga acgcgggcga agcggacgag actcagagag
     1621 ccgtcccaat ccacacttcc cacggaatcg cccgcctggc gcgaaaacac cgaagaagcg
     1681 gcattcacca ccgtatggac atcgtgacac gccgcggaca cgctgtcgat atttttcagg
     1741 tccccgccga agagttccac tccgatttca cggagactat tcaatcgttc cggattggct
     1801 gtcggccgga ccagcgctct cacaggcatt ccgcgctccc gcagccgccg gcagatttct
     1861 tgtcccaata gacccgtggc gccaacaacg agaatcatcg cccgccctcc tgaaaatttg
     1921 cacttcaatg cccagttgca ttcgactgcc ttatttatac cggaaaatct tgttcgcgaa
     1981 aagcaataat cgttgtatta tggggtcggc atatgccgcc gcctggcggt ggagttgctg
     2041 ggatgttccc cgaggggcaa ctactccatg tcgaccgtcg ctgaaacgaa tctgatccaa
     2101 aaaatggaac cgctttcgcg cgggcgcacc gatttgcgca agcgcaaacc gaaacagatt
     2161 gcgattatca acgagaactg caccggatgc gccggttctc cggcgtgcgt cgcctactgc
     2221 ccggtggaag cttgtatgtt ttggataccg gacgaggagt attctccgtt cggcagaatc
     2281 gaagtagaca agtccacttg cattggatgc gcgaagtgca ccagcaaggg cccggacggc
     2341 acattcctcg acggttgtcc gtgggacgca atcgaaatgg tcgccatcgg tgaatgggaa
     2401 cagagtcatg gagtcgttct acccgccgaa ccggaccgcc ccgctacgga atgggactac
     2461 gtccggcgcg attacgtata gcttccgccg ttaatgcggc ctgttgccgg atgcggccat
     2521 ccatgtccgg cagttcgccg cggtactgat cgatctgttc cagatcgctc tcacttccta
     2581 cgaaatacag ccctcgcagt gcctcccaga cttccgacga atccgcacga acaaacagga
     2641 cctcgtcccc gcgtttgaca cgcgagccgt cctttacggc gagactttcg aggcgcccgg
     2701 aaagaggggc tcgcagaggg atctcttcac cctgagagcc tgtaatgctg ccaacagcga
     2761 tacccgccac caccgcctgt ccctccggtg tgtcgagagc gacgacgccg tcaaccggtg
     2821 cacgaatgga ctgggggcga aggatatcga gaagctccgg ccgtccccgg ccatcgccaa
     2881 accggaccag cgacagcgcg gcattccggc ggacactcgg ctcggggtcg gaaagaagct
     2941 tcagcagcgc atcgtgaaag atctgcgagt tgttgtcctg gcccatcacc caggcggcgg
     3001 tgctacgaat ctccggcctg gaattccgcg ccgccgcaag gatctgcgga taccatccgg
     3061 agacggagct cccgccgcgc gacatgcggt ccgatatttg ggaaagtgcg tgttggatgc
     3121 ggcggggatg ttgctcgtcc tggagatacc ggctgaggtc ttcgtccgtc agttcacggc
     3181 cgaaccatgt ttgataccaa aagatcagag gcatggacac aagcgagatg gcgaccgccc
     3241 acattacgat aagaagccag cgccggggag cccgggcttt ggctggctgg ggcggcggga
     3301 tgctattcgg tgtcattact gtttctgcga ggccgcctgc gcaatctgct gttcccgatc
     3361 tgtttgcggt ttacgccgca ctcgcttgag gagatcgcgc ggaagagcgg ctcgaagaga
     3421 ttgaagaggc atcagggaaa acagaacagc caccacgagg aaagagaata tccaaaacag
     3481 gccatcaatg ccgtcctgag aagcgcgccc tgtgatcaga taccaggcca ccgcgcccag
     3541 cgcgcccagt gcaatcaccg aacaaatcag gttcagaatc atttgttctc tcatcgttag
     3601 tgccatcatt caccatccca gcctcttcgg ccgtctgttt gccctgaaac cggaaggcca
     3661 gcagaaaaac gctgctaaac agggccacgg ccggaatgag cagtatcaga attgcggtat
     3721 tcagtgtgct ggcaaccacg ggcccctgtc cggccagcgc ggtacggcac atcgaacact
     3781 gaccgaacaa cggcccgccg caactggctg cgagaatgcc aacaatcatt gctacacgca
     3841 tatccaccta tcgaaccctc agctcgaaca acc
//
LOCUS       DASTMK010000283        17480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_110064, whole genome shotgun sequence.
ACCESSION   DASTMK010000283 DASTMK010000000
VERSION     DASTMK010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17480
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110064"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VFQ79_12210"
     CDS             <1..1466
                     /locus_tag="VFQ79_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140475.1"
                     /translation="PVLLLACAALYRQAANRLFPFTFLLCLLAAVSAHGFVLSLAIGV
                     MFHVSVASGKKSLPGRDKRPFVVAAFTYYVVLLLLAVLAWPAADGIFFAGPNLAPQHF
                     FTVAGATLRDAFTGYTIPSGIIIALSLPFLRRGGTLLYFVLVSILLIGVNAIIYSNVW
                     HYGVLFLSWLFAVWIAALTRKPTLPVLIALATVVTIQCSWTIASARYDWSHAYSGSHE
                     AAGALHAAGIPRHQLLGIGFSSTSIQPYFSSNVFSNFNDGREQAYWDWSSRNRANDPL
                     VLFSSPDTYVMAGYKAEAERDRWAALARLSGRERIGRFEGNVFWRNGILEPEAFDFYG
                     PPAAGTDLSAASTIKMSDPSASGQLLSGFYPIESDAWRWTAGTFALVLKRPEGAGGSG
                     AKLILKLMIPEAQIKRLGTMTLRANVDGQELPPEAYSRPGTYVFARQIASHALRNRLV
                     PVRFRFDKFAPPSKSDSRELGAIVSLASLETNPGAGQ"
     gene            1463..3223
                     /locus_tag="VFQ79_12215"
     CDS             1463..3223
                     /locus_tag="VFQ79_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140476.1"
                     /translation="MTFPQAVRFFIDQTTQFVSPVNVAAEWVLVVLAALAVAAPKVGS
                     GRLLARLRDALRRFARRKSAAIVLCGVLPMALRLSMLWFAPAPEPSIHDEFSNLLLAD
                     TLAQGRLTNPAHPMWMHFESIHIIQQPTYNSMYPPAQASLLALGQVLFREPWAGVVIG
                     VGLMCAAICWMMQGWLPPAWALFGTLIAILKIGVVGLWMNTYVSASAPAIGGALLLGA
                     LPRLRRKLRAGHFAVFALGLVILMNSRPFEGAILGSVAALCLIPALLRQFTKSPLESI
                     KLVAPAAIVLASGVVFTGYYNYRVTGNPLRLPYQVNRDTYGWPENLGFLAPKKLELRH
                     KVLQNMYIKEVHNRDAYKTMPGAIDSIKRRVFENWTFFIGPVLTVPLLFFPWIFRSRR
                     TRPLVIFVAVAAFVNLFQLVLYPYHLAPLVAAIFVIVAQGTRYLYHWLSRQDGQRALR
                     FAFLVPLCLILVGAMKQNAVRLWLPMAYWEHAFEPHRDARAYIQNWLTARPGKQLVIV
                     RYAKHHSVDQEWVYNRANIDESKVVWAREMDPVSDTRLVRYFSDREAWLLEADIYPTR
                     VVRWRGRTTYTPAVRETASR"
     gene            3220..4848
                     /locus_tag="VFQ79_12220"
     CDS             3220..4848
                     /locus_tag="VFQ79_12220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0140477.1"
                     /translation="MKPASARGRLQVAAVFAAALLVLSFNLWNTGIASGWIDPVLRLG
                     AQDEAVYTHEAIRMITHGDWLTPTLAGRWSFEKPPLLMWLSALSMKMFGIGPFTARIP
                     PVLAGALVAVLCFVAGRAARSPAAGLGAALLCVSSPLLFTMSRHNMTDILLTASALGA
                     FAAQALDPSLLRSRSRIAFTLAIAAGILTKSVAGLLPVFAALLFAALSPHDTGARLRR
                     VAVLSTCAVALASPWFLYHLAIHREWFLADTGFQILTTGMRPHQTAPESHISFYLSRL
                     FYAAPVALILALTGIPAMMGALRRRADVPLLLVSNLLVLGLALMVFRFRSEQYLTPVV
                     PLLALVAVISSPLFQRRFAPVVLAGIAVLSGVKVSNPGQPWGMSYRSGTTIPAAGMLS
                     EYCEERRGNGLYILGVNEEFYALALPLARVHYGWIDPDGIVEDGRPHLFYLGIIRHAG
                     SPSHSSLYAERLRAWGLDSSEPLGTAIVARTAGELAVLVAEHPESDFLLSREVVEKLD
                     QHTSHEIRRITPELVLLYSKTKMPPVTPRWTCAM"
     gene            complement(4889..5362)
                     /gene="bfr"
                     /locus_tag="VFQ79_12225"
     CDS             complement(4889..5362)
                     /gene="bfr"
                     /locus_tag="VFQ79_12225"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEU0140478.1"
                     /translation="MRGNENVINELNAALRAELIAIVQYMVHAEMCHNWGYQRMGDYI
                     RKQAIDEMRHAEHLIERILFLDGTPQVGIGLDPKIGDTVKAQLENDLAAELEAVQQYN
                     HSVQVCRDAGDNGSRELFEAMVKDEEEHTDFLEAQRDLIAQTGYENYLAQQIKGG"
     gene            5399..6232
                     /locus_tag="VFQ79_12230"
     CDS             5399..6232
                     /locus_tag="VFQ79_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0140479.1"
                     /translation="MVVDIEGVPLHLAHPDELPGEWVGQEEVMRQLLAAWLVIDPQDM
                     PMNPRLIGKPGVGKTTLAYAAAKRLGRDIYIMQATVDTRPEDLIVTPVIEGASRLRYV
                     ASPLVTAMVRGGIAILDEGNRMSEKSWASLAPLLDTRRYVESIVAGIKIKAHPMFRMV
                     ATMNDDASTFDLPEYIHSRLQPQIFIDFPERDEEYQILKENLPFADDQVLQYVTEFLQ
                     QAHASDERYTVRDGINIGRFAMKLKRCANDNIQERQALYLAVSETLGEEALRYVPRVP
                     A"
     gene            6210..8048
                     /locus_tag="VFQ79_12235"
     CDS             6210..8048
                     /locus_tag="VFQ79_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140480.1"
                     /translation="MSPEFPPDFGKEFLEAGSLRRGHLHYFPVVPGRMEFAIEVRRAI
                     LDQRPAIVAIELPVSLERHYLRAVGRLPELTVLIYADENEEDRGIYVPVEPADPFTEA
                     VRTALEIEAEVLFLEPGGGERPHLPDRYPDPYSIRRIGLEKYIEAYRVYPQPRNEEIA
                     GHAARMAWRLQGADPLPDVLVVVSLNLLDPLLDAMEEPQEKPRIRSSAVDLVNPHPDC
                     LAEITAEYPYLQERYEEFRTSMECWDTIDRPRTQYDLLREAEQAYQKNTGERIQHWQR
                     RMMARFTRNLAWLGSDLVAGLFDIIVAARSIVDDNYGWEVWDMAGRYRHQREKSDLET
                     VNLSGEEVWLNTRKLRLRRRLPRPKQRLKPMGLKERKKEKFPGEWAQQLDGNSICSYP
                     PEDLVIEGYGKFLKKQARHILSEERMRTEPFTTSVLDGIDLRETIRNWHEKKIYVRQF
                     DKIAGEVGSVIVIFDEDPDDRYTYLTTWLGENQNESDMAFYSTHPFENLVGPGIGRAE
                     YGGFLMSLPPRRMYDVWGDPDYDFALNKSERLLMAGLDYSIHKHVVYVASKPPRSIFR
                     SIAARIGRTILYIPIGQLSPAKLKKIRVVHVLDSHKRREEASQYIW"
     gene            8187..11426
                     /locus_tag="VFQ79_12240"
     CDS             8187..11426
                     /locus_tag="VFQ79_12240"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0140481.1"
                     /translation="MNARSLFAAVFLLFASSALCLGQRTTAQLTGTITDATGAVVPHA
                     QLTVTHESTGLQRDSVSNELGYYTIPLLPPGTYRIAVKVPGFRPITRSGVVLEVDQVA
                     RVDFALEVGTLAEAVEVTANASRVDTQTATLKEVVDQHRIRELPLNGRDATQLVLLLP
                     GVYGTDDTSGLRQGGSARSIVQPGISSNGARSNMVNYTLDGAFHNDTYTNAGMAMPNP
                     DALQEFSVQTNSFSAEFGRSAGAIVNAVTRSGTNEIHGTLFEFHRNEAVNARNFFASG
                     SDGLKRHQFGGTLGGPVYLPRIYDGRNRTFFFFSQQETRQKQTPSDLSVTVLTEAQRR
                     GDFSARTQPIIDPLTGSPFPGNIIPADRINPVTRNFIDQLLPLPTEPATGLLRYKAAN
                     NNDERQIVLKVDHQFSSKDTLTARYLYNFYHQFANDVPLVFATRPDRQTPAHNISLNH
                     VHIFRPNLLNQAQVSINRRKDLGVPVWNTSLADLGMRNVYSDKPHPTIVVGVTGAFSI
                     ETTEWIITEPNVYTFSDTLRWTRGGHEMTFGFEYRFQTLKKLYRWLMDPTMSFAGNYT
                     GYGVADFYLGLPSRLRQKAYGEFADLEAPGYSTFFQDNIRVTPRLTLNLGVRFEPTIN
                     YVDIHNRGSVFRQGQKSQIYTNAPTGLLVIGDPGVPRGQAENDINNIAPRFGFAWAPF
                     GNGKTSVRGAYGIFYDSSPMSAINNGITDSPPFALTIDYSPSPGPFDDPYNGQNPLPM
                     PSPPPSDIDFPLPLSMSTYPQKLRAAYLQSWHLTFERELRQDWMARIAYAGSKGTGLL
                     QGWQYNPGIYIPGKSTLLNTNERRPLAPEWSSIGIREGIGNSNFNSLQLTLDKRFSKG
                     FTAQMNYTWGKSIDSGSGAGTLWPSFNNPFNFRQSRGLSDFHHEHRFVASGLWELPRL
                     MGMPALAKGVLGGWSLSGAMLLESGRPFSVLSGRDNSMSGVGADFADLAGDTSRSARQ
                     DPNRDPVLEWFNTQAFVQNAEGTFGNSGRNIIFGPGHANVNLSVSKSFPIAFLGEASR
                     IQFRGEFFNLFNRVNLLSKRSESLTSGTYGRITSAADPRILQFALKWHF"
     gene            11446..14997
                     /locus_tag="VFQ79_12245"
     CDS             11446..14997
                     /locus_tag="VFQ79_12245"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0140482.1"
                     /translation="MLSLAFFAVLALTIDGKLDDPFWKPVTPQTLESPAGGRIRAVVA
                     GRHLYIAAEVPEPTGRVTARSIGRNPAWEDEDLLRISAGATIGYTDRVLHVNPFGAYS
                     LERAGHPVWTSLSVFPYSDEREAPVVPVNIDKFLVATRVGEKGWTAEIAFPLSELSAP
                     GPDTIYAMIERIRAQRPGVPQQVWRWPGPGPAARIPVAPDIPWETPPARFEPPLLGNS
                     EPPLEIGRAGTLPALESKWQDGAWAHAPVWKLLLDEHPLRAPRLATEVKLLHDGRTLA
                     VMARCVEPDGISAEVKENDGAVMQDDSFHVYLATSRSVYVQVVANALGYLRDAAGFSG
                     GSRISRPREWDSGTRVHVEREAGVWTARLDIPLEPVAEVLGESTVPADWRVLLMRFRR
                     GRPGDARETSVLPVIQSDTALCPARYRRLRLAAKATAPGNTQGQAAQTAGTPELALVL
                     TREQRRQMGLATMLDRHIRARVRKHLEADRDEWNQVNSRGDWERFRNPRLNAMRAWMG
                     EFPARTPLEASVTKEYRGHGYRRQDLVYRSRPGLWVTANLYLPEKPSGQLPGIVIVHS
                     HHRPRTQAELQDMGILWARQGAAVLIMDQIGHGERIQTYPWNREGYRSRYSTGMQLYV
                     AGESLIKWMVWDIMRGIDLLLERPDIDREKIIMLGAVAGGGDPAAVTAALDPRIAAVA
                     PFTFGESLPELGPGGSPFPSDLAVPGWGSWETTRNLPHSIGKRFFPWTICASVAPRRF
                     VYSYEMGWEVEKVPAWARYRKVFGFYNALDNLDEAHGFGTFPGPGECANIGPAQRKTL
                     YPELERWFGIPIPDNEPDDRRPEAELAALTVETAAKLDMRMIHQLASEIAEAKLKTAR
                     AELARMDSTGRRKWLQQQWAAKLGDIHPNPAPKAIVHWMKPSASGEAEGITLDVESGI
                     TVPLVLLKASTREARQPVAIVVSQGGKEQVLAHHRAEIDRLLAAGIAVCLPDVRGVGE
                     TSPDPRRFTSSQNISLAATELMLGNTLPGARLKDLRTVLKYLQGRQEFDPQRIAVWGG
                     SGAPVNKGRLLLDETPGWLVGPEIQHQAEPLGGLLAILAGLYEDSVKAVAVRRGLAAY
                     LSLLEDRFTYVPSDVVFPGIAEIGDIADLAAVLSPRPLLLDGLVDGRNRAVSEPELRA
                     TFSPTLEAYRGQPEHLRLRATTAEPGVAEWLIQVLGR"
     gene            complement(15008..16609)
                     /gene="cobA"
                     /locus_tag="VFQ79_12250"
     CDS             complement(15008..16609)
                     /gene="cobA"
                     /locus_tag="VFQ79_12250"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEU0140483.1"
                     /translation="MRARAPSWRLYTEPQDPKKPLPHGRGSETDIAETEPRTSASGKV
                     FLVGAGPGDPDLIPVKGREILSKASTVLYDNLANAMLLDLAPETAERIYVGKKKAAHN
                     ITQEEICALLLEKARPGHVVVRLKGGDPFIFGRGGEEAEALAEAGVPFEVVPGVTTAL
                     GIAAYTGVPLTHRGHTSAVSFVSGHNVDAIDWTKVGQAETLVIYMGLTSFPDIARNLI
                     ANGRSPDTPAMAVRWATRPDQVRIAGTIATLPRLIEERRMKPPATIVVGEVVQLGARL
                     NWFERLPLFGQTIVVTRAASQAGDLSARLRSLGACVRELPAIQIGPPADSGPLDAGIA
                     HLGDYDWILFTSVNGVRFFLEALDRSRVDLRSLRARICTIGPATRRAVEELHLKVDLT
                     PEEYVAESLVASLATADVAGKRILLPRAAVARDLVPAELSRMGAQVDVVEAYRTTMPE
                     SVVPRARELFGSGTPVDWITVTSSSTVKNLVCAVGAETLRGFKLASIGPVTSATAREL
                     GLDVAVEAKPYTIDGLVEAIQNSSR"
     gene            complement(16557..>17480)
                     /gene="hemC"
                     /locus_tag="VFQ79_12255"
     CDS             complement(16557..>17480)
                     /gene="hemC"
                     /locus_tag="VFQ79_12255"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU0140484.1"
                     /translation="MLRIGSRGSKLALWQAQWVYSALERQGEQCEIEIIRTTGDNITD
                     VSLARLGASTGVKGLFTKEIEEALLGGRIDLAVHSLKDMPTDLPDGLVLAAVPEREDA
                     RDVLVGSTLARLPRNAKVGTSSLRRAAQLRACRPDLVIEPLRGNIDTRLRKLDEGQYA
                     GIVLAAAGLKRMGWEGRIAEPLPPEVMCPAVGQGALAIETRADEGHACQACRKLEHAQ
                     TRAAVTAERALLRSLGGGCQVPIGGHAVVEGGRVHLRAVVISPDGREIVRRENSGAVA
                     EAEHVGAELGRDILDAGARAILEVVYGTSGP"
BASE COUNT         3334 a         5370 c         5233 g         3543 t
ORIGIN      
        1 tgccggtgct gttacttgcc tgcgcggcgc tatacagaca agctgcaaac cggctgtttc
       61 cgttcacctt tttgctctgt cttctggcgg cggtgagcgc tcacgggttc gtgctgtcgc
      121 tcgctatagg agtgatgttc catgtatctg tagcttcggg gaaaaaatct cttccgggcc
      181 gggataaaag gccgtttgta gttgccgcgt tcacttacta tgttgtgctg ttactgcttg
      241 cagtcctggc ttggccggcg gcggatggga tcttttttgc cggtccgaat ctcgctccgc
      301 aacatttttt caccgttgca ggcgcgacac tccgggacgc atttacgggt tataccattc
      361 cctccggtat catcattgcg cttagccttc ctttcctccg ccggggaggt acactgctct
      421 attttgtact ggtgagtatt ttactgattg gcgtgaacgc gatcatttac tctaacgtct
      481 ggcattacgg tgtattgttt ctgtcgtggc tgtttgccgt ttggattgct gcgcttacca
      541 ggaaacctac gctgcccgtc ctgatcgcgt tagccactgt agtcaccatt cagtgcagtt
      601 ggaccattgc ttccgcccgt tacgattgga gtcacgctta ctccgggagc catgaagcgg
      661 ccggcgctct acacgcggcc ggcattccgc gccatcaatt gcttggcatt ggattttctt
      721 cgacaagcat ccagccctat ttctcatcga atgtgttttc aaacttcaac gacgggcggg
      781 aacaggcata ctgggactgg tcaagccgga accgggccaa tgacccactg gtgctgtttt
      841 cgtcgccaga cacgtatgtc atggccggtt acaaagcaga ggcggaaaga gaccgatggg
      901 ccgcactggc ccggttgagc ggacgggagc ggatcggccg tttcgaggga aacgtttttt
      961 ggcgcaacgg gattttggag cccgaggcct tcgatttcta cggccctcca gccgccggga
     1021 cggatttatc cgcggcaagc acgatcaaaa tgtccgatcc atcggccagc ggccagctcc
     1081 tctccggctt ttatcccatt gagtccgacg cgtggcgctg gacggcgggg acgttcgcgc
     1141 tggtattgaa gcggccggag ggtgccggcg gctccggcgc caaactcatt ctgaagttga
     1201 tgattccgga ggcgcagatc aaaaggcttg gcacgatgac cctgcgggcg aatgtggatg
     1261 ggcaggagtt gcccccggaa gcctactccc ggcccggcac ttatgttttc gcccggcaga
     1321 tagcatcgca tgcacttcga aaccgcctgg tacctgtgag gttccgcttc gacaaattcg
     1381 ctccgccctc gaaaagcgac agcagggagt tgggggccat cgtctcgcta gccagtctcg
     1441 aaacgaatcc cggcgccggc caatgacttt cccgcaagcg gtgcgctttt ttatcgacca
     1501 gacaacgcaa ttcgtttctc cggtcaatgt tgcggcggaa tgggtactgg tggttctggc
     1561 tgctcttgcc gtggcggctc ccaaggtggg ctccggccgc ctgcttgcgc gtcttcggga
     1621 tgcgcttcgc cggtttgccc ggcgcaagag tgccgccatc gtactttgtg gcgtgttgcc
     1681 catggcactg cgcctctcga tgttgtggtt cgctccggcc ccggaaccga gcattcacga
     1741 cgagttcagc aacctgctgc tggccgatac gctggctcag gggcgtttga ccaacccggc
     1801 gcatccgatg tggatgcact tcgaatcgat tcacatcatc cagcagccga cctataactc
     1861 tatgtatcct ccggcgcaag catcgctgct ggcgttggga caggtattgt ttcgcgagcc
     1921 atgggctggc gttgtcatcg gcgtggggct gatgtgcgca gccatttgct ggatgatgca
     1981 gggatggctg cctcccgcct gggcgttgtt cggaacgctg atcgccattc tgaaaatcgg
     2041 ggtggttggt ctttggatga acacgtatgt cagcgcctcc gctccagcca tcggcggagc
     2101 gctgctgttg ggagccctgc cgagactacg gcggaaactt cgcgccggcc acttcgcagt
     2161 tttcgcgttg ggcctcgtca tcctgatgaa cagccgcccg tttgaaggtg ccatactcgg
     2221 atcggtcgca gccttgtgcc tgattccggc actgctgcgg caattcacga aatcgccact
     2281 cgagagcata aagctcgtag cgccagcggc aattgtgctc gcctcggggg ttgttttcac
     2341 cggctattac aactacagag tgacaggcaa cccgcttcgc ctcccttacc aggtgaaccg
     2401 cgacacctat ggctggccgg aaaacctcgg attcctcgct ccgaagaagc tggaactacg
     2461 ccacaaagtg ctgcagaaca tgtatataaa ggaggtccac aaccgggacg cttacaagac
     2521 gatgcccgga gccattgata gtataaaaag gcgcgtgttc gagaactgga cgttcttcat
     2581 cggtcccgtg ctgactgttc cgctcctatt cttcccctgg atatttcgca gcaggcggac
     2641 tcgcccgctg gtaatttttg tggcggttgc cgcattcgta aatctctttc agctcgtgct
     2701 gtatccctac cacctggctc cgctggtggc ggccatcttc gtcatcgtcg cgcaagggac
     2761 gcggtatctc tatcactggc tttcccggca ggacgggcag cgagcgctgc gcttcgcgtt
     2821 cctggtcccg ctctgcctga tcctggtggg cgcgatgaag cagaacgcag tacggctgtg
     2881 gcttccgatg gcctattggg aacacgcttt cgagccgcac cgtgatgcgc gcgcctacat
     2941 tcagaactgg ctcactgcgc gccctggaaa acagcttgtt attgtgcgat acgcgaagca
     3001 tcattccgtc gatcaggaat gggtatacaa ccgggcgaac atcgacgaga gtaaggtcgt
     3061 gtgggcccgc gagatggatc cggtatcgga cacgcgcctg gtccggtatt tttcggaccg
     3121 tgaagcctgg ctgctcgaag cggacattta tcccacccga gtggtgcggt ggcgcgggcg
     3181 caccacctac accccggcgg tgcgggaaac ggcgagccgg tgaagcccgc ttcagcacgc
     3241 ggcagactgc aagtcgcagc ggtttttgca gccgctcttc tggtcttatc tttcaacctg
     3301 tggaataccg gcattgccag cggctggatc gatcccgtgc tgcgtcttgg agcgcaagac
     3361 gaagcggtct acacgcacga agcgattcga atgatcacac acggcgactg gttgacgccg
     3421 acgctggcgg ggcgctggtc cttcgagaag cccccgcttc tgatgtggct ttcggctctc
     3481 tcgatgaaga tgttcggcat tggccctttc accgcacgga ttccacctgt tcttgccgga
     3541 gcgctggttg cagtactgtg cttcgtcgcc gggcgtgccg cgcggtcccc cgcggcaggg
     3601 cttggagccg ccctgttatg tgtatccagc ccattgctgt tcacgatgtc gcgtcataac
     3661 atgacggaca tcctgctgac ggcttccgca ctcggcgcat tcgcggcgca ggcgctggac
     3721 ccatctcttt tgcgaagccg gtcccgcata gcatttactc ttgccatcgc cgcgggcatc
     3781 ctgacaaaga gcgtggccgg gctgctgccg gtttttgcgg cactgctgtt tgcggctctt
     3841 tcaccgcacg acactggcgc gcgcctgcgc agggtcgccg tgctatccac gtgcgccgtt
     3901 gcgctcgcct ccccgtggtt cctttaccac ctcgccatac acagggaatg gtttttagcc
     3961 gataccggat ttcagatttt gaccacggga atgcgcccgc accagacagc gccggaaagt
     4021 cacatctcat tctatttgtc gcggcttttc tacgccgcgc ctgtagccct gatactggcg
     4081 ttgaccggca ttcccgcgat gatgggcgct ttgcgtcgcc gtgccgatgt gccgctgctg
     4141 ctagtttcca atctgttggt actgggcctt gcgctgatgg tgttccggtt tcgttccgag
     4201 cagtacctga cgccggttgt gccactcctc gcgctggttg cggtgatctc ttcgccgctt
     4261 ttccagcggc gattcgcgcc ggtggttctc gcggggattg cggtcctgtc cggcgtcaaa
     4321 gtgtcgaatc ccggacagcc ttggggaatg tcgtatcgct ccggcacaac gattcccgcc
     4381 gccggcatgc tctcggaata ctgcgaggaa cgccgcggca acgggttgta cattcttggc
     4441 gtaaacgaag aattttacgc gttggccctg ccgctggcgc gggtccatta cgggtggatc
     4501 gatcctgacg gaatcgtcga ggatggacgg ccccacctgt tctacctggg aatcatacga
     4561 cacgccggat ctccttcgca ttcgtcctta tacgcggaga ggcttcgcgc ctgggggctg
     4621 gattcgagcg aaccgctggg taccgccatc gttgcgcgca ccgcgggcga actggcggtg
     4681 ctggtggccg aacatcccga aagcgacttt ctgctttcca gagaagtcgt cgaaaaactc
     4741 gaccagcaca cgtcgcacga gatacgccgc atcacgccgg aactggtgtt gctctattcg
     4801 aagacaaaaa tgccgcccgt cacgccgcgc tggacctgcg cgatgtaacg ggcgcctacc
     4861 gcttgctcgc gcgcgcggtc cgtaacggct acccgccttt gatctgctgt gccaggtagt
     4921 tctcgtagcc ggtttgtgcg atcaagtcgc gttgggcttc gagaaaatcg gtatgctcct
     4981 cttcgtcttt caccatggcc tcgaagagct cacggctgcc gttgtcgccc gcatcccggc
     5041 agacttgaac agagtggttg tattgctgca ccgcctccaa ttcggcggcg aggtcattct
     5101 ccaattgggc tttcacggta tcgccgatct tcggatcaag accgatacct acttgcggcg
     5161 ttccgtccag gaagagaatc cgttcgataa ggtgctcggc atgccgcatt tcgtcgatcg
     5221 cctgcttgcg gatgtaatcc cccatcctct ggtagcccca gttatggcac atttcggcat
     5281 gcaccatgta ttggacaatc gcaatcagtt cggcgcgcag cgcagcgttt aactcgttga
     5341 tgacgttctc gtttccacgc atactgttcc tacagagtag cagctaagat agggagatat
     5401 ggtagtcgac attgaggggg tgccgcttca tctcgcacat ccggacgaac tgcctggcga
     5461 gtgggtggga caggaagaag tgatgcgcca gttgctggcc gcctggctgg tcatcgatcc
     5521 acaggacatg ccgatgaacc cccggttgat cggcaagccc ggcgtcggaa aaacgacgct
     5581 cgcctatgcc gccgcgaaac ggctcggccg cgatatctat atcatgcagg cgacggtaga
     5641 tacgaggccg gaagacctga tcgtcactcc cgtgatcgaa ggcgcatccc ggctccgtta
     5701 cgtagcttct cccctggtaa ccgcaatggt gcgcggaggc atcgccattc tcgacgaggg
     5761 gaatcggatg agcgagaaaa gctgggccag cctggctcct ttgctcgata cgcggcgcta
     5821 tgtcgaatcg atcgttgcgg gcatcaagat caaggcgcac ccgatgttcc ggatggtggc
     5881 gactatgaac gacgatgcat cgacgttcga cctgccggaa tatatccact cgcgtcttca
     5941 gccgcagatc ttcatcgatt ttccggaacg cgacgaagag taccagatcc tgaaagaaaa
     6001 tctgccattc gccgacgacc aggttctgca atacgtcaca gagttccttc aacaggcaca
     6061 tgcctccgac gaacgctaca cggtgcggga tggcatcaac atcggccggt tcgcgatgaa
     6121 gctgaagcgc tgcgccaacg ataacatcca ggagcggcag gcgctgtatc tggcggtgtc
     6181 tgaaaccctc ggtgaagagg ctttgcggta tgtcccccga gttcccgcct gatttcggca
     6241 aggaattcct ggaggcgggg agtctgcggc gcggacacct ccattatttt ccggtggttc
     6301 ccgggcgaat ggagtttgcc attgaagtgc gccgggccat cctggaccaa cggccggcca
     6361 ttgtcgccat agagcttccg gtttccctgg agcggcacta tctccgcgcg gtcgggcgcc
     6421 tgccggaact caccgtgctg atttacgcgg acgagaacga ggaagatcgc ggcatctatg
     6481 tacccgtgga gccggccgat ccgttcaccg aagccgtgcg caccgcgctt gaaatcgagg
     6541 cggaagtgtt gttcctcgaa cccggcggag gagaacgccc gcatctgccg gaccgctacc
     6601 cggacccgta ctccatccgg cggatcggtc ttgaaaaata cattgaggca taccgagtgt
     6661 acccgcagcc gaggaacgag gagatcgccg ggcatgccgc gaggatggcc tggaggctac
     6721 aaggcgccga tccactcccg gacgtattgg tggtggtttc cttaaacctg ctggacccgc
     6781 tgttggacgc gatggaagaa ccacaggaaa agcctcgaat ccggtcttcc gccgtagacc
     6841 tggtgaaccc gcatccggac tgtctggccg aaatcactgc cgagtatccc taccttcagg
     6901 aacgttatga agaattccgg acttccatgg aatgctggga taccatcgat cgcccccgta
     6961 cgcaatacga cctgctgcgc gaagccgaac aggcgtacca gaaaaatacc ggcgagcgaa
     7021 tccagcattg gcagcgcagg atgatggcgc gcttcacgcg caatctcgca tggctcggca
     7081 gcgacctggt ggcggggctg ttcgatatca tcgttgccgc ccggtccatc gtagacgaca
     7141 actacgggtg ggaagtgtgg gacatggccg gcagataccg ccatcagagg gagaagagcg
     7201 acctcgaaac cgtcaacctg tcgggcgaag aggtatggtt gaacacgcgc aagctgcggc
     7261 tgcgccgccg ccttccccgg ccgaaacaac gcctgaaacc catgggtctg aaggaacgga
     7321 aaaaggaaaa gtttcccgga gagtgggcgc agcagcttga cggcaactcc atctgctcgt
     7381 acccgcccga agacctggtg atcgaaggct atggaaagtt tctgaaaaaa caggcgcggc
     7441 acattctgtc ggaagagcgg atgcgcaccg agccgttcac cacgtcggtc ctggacggaa
     7501 tcgacttgcg cgaaaccatc cgcaactggc acgagaagaa gatctatgtg cggcaattcg
     7561 acaagatcgc gggcgaggtg ggttcggtga ttgtgatctt cgatgaagac ccggacgacc
     7621 gctacacgta cctgacgaca tggctcggtg agaaccagaa cgaatccgac atggcattct
     7681 attcgacgca cccgtttgaa aacctggtgg gaccggggat tggacgggcg gagtatggcg
     7741 gatttctgat gagcctgccg ccgcgccgca tgtacgacgt ttggggcgat ccggattacg
     7801 attttgcgct gaataaatcc gaacgcctgc tgatggccgg gctggattat tccatccaca
     7861 agcatgttgt ttacgtggca tcgaagccgc ccaggtcgat cttccgctcc attgcggcgc
     7921 ggattggacg gacgatccta tatattccga tcggccagct ttcgccggca aaactgaaga
     7981 agatccgcgt cgtgcacgtg ctggacagcc acaagcggcg cgaagaggca agccagtata
     8041 tctggtgacg caagggcgcg gtgggcgctc gactcgaatc cccgccggtc ctgaatttca
     8101 cctcggaaaa tcgttgacaa tttactgaat tcacaataaa gtctttggaa gttctctggt
     8161 ttctaatctt ttcagaggtt aaactgatga acgcaagaag tctgtttgcg gctgttttcc
     8221 tgctgttcgc atcttccgct ctctgcctgg gccagagaac gacggcacaa ctgacgggga
     8281 ctattacgga cgcgaccgga gctgtggtcc ctcatgctca actgacagtc acgcacgaga
     8341 gtacgggatt gcagcgagac tcggtcagca acgaactcgg atactacacg ataccgctgc
     8401 tgccgcccgg cacataccgc atcgctgtga aggtgccggg attccggccg attacccgtt
     8461 cgggggtcgt ccttgaagtg gaccaggtgg cccgcgtcga tttcgcgctg gaggttggaa
     8521 ccttggcgga agccgtggaa gtgaccgcca acgcctccag ggtagacaca caaaccgcca
     8581 cccttaagga ggtagtggac cagcaccgca tccgcgagct gcctctgaac ggccgcgacg
     8641 cgacacaact ggtcctgctg ctgccgggcg tttatggcac cgacgacacc tcgggattgc
     8701 ggcagggtgg ttccgcgcga agcatcgtac aacccggtat ttcgtctaac ggcgcgcgca
     8761 gcaacatggt gaactacacc ctggacgggg cgtttcacaa cgacacctac acgaatgccg
     8821 gcatggccat gccgaacccg gatgcccttc aggaattcag cgtgcaaacg aacagcttta
     8881 gcgcggagtt cggccgaagc gcgggcgcca tcgtcaacgc cgtcacccgc tcgggcacaa
     8941 atgagattca cggaaccctc tttgaattcc atcggaacga agcggtcaac gcacggaact
     9001 tctttgcgag cggaagcgat ggcttaaagc gccatcaatt cgggggcacc ctcggcgggc
     9061 ctgtgtatct tcccaggatc tacgacgggc ggaaccggac gttcttcttc ttctcgcagc
     9121 aggagacacg ccagaagcaa acgccgtccg acctcagcgt caccgttttg accgaggccc
     9181 agcggcgggg agacttctcg gcacgaacgc aacccatcat cgaccccctg accggaagcc
     9241 ccttccccgg caacatcatt ccggcagatc ggatcaaccc ggtgaccagg aactttatcg
     9301 accagttgct cccgcttccc accgagccgg caaccggcct tttgcgttac aaggcggcga
     9361 acaacaatga cgaacggcag attgtcctga aggtcgatca ccagttcagc tcgaaggata
     9421 ctttaaccgc gcgctacctc tataactttt atcaccagtt cgcgaatgac gtgcccctgg
     9481 tattcgccac acgcccggac cggcagacgc ccgcgcataa catatccctg aaccacgttc
     9541 acatcttccg ccccaacctc ttgaaccagg cgcaagtttc catcaaccgc agaaaggatc
     9601 tcggcgtgcc ggtctggaac accagtctcg ccgatctcgg aatgcgcaac gtctactccg
     9661 acaagccgca tccgaccatt gtcgtcggcg tcacgggggc cttttccatt gagacgacgg
     9721 aatggatcat caccgagccg aatgtctata cgttttccga cactcttcga tggaccaggg
     9781 gcgggcacga gatgaccttc gggttcgagt atcgctttca gactctgaaa aagctctatc
     9841 gctggctgat ggatcccact atgagcttcg ccgggaacta taccggttac ggagtggccg
     9901 atttctacct cggcctcccg tcacggctgc ggcaaaaggc ttatggcgag ttcgcggacc
     9961 tggaagcccc gggctacagc accttcttcc aggacaacat acgggtgact ccccgcctga
    10021 ccttgaacct cggcgtgcgg tttgaaccta cgatcaacta cgtcgatatt cataaccgcg
    10081 gaagcgtctt ccggcagggc caaaagagcc agatctacac caatgcgccg acagggttgc
    10141 tggtgatcgg cgaccccggc gtgccgcggg gccaggcgga aaacgacatc aacaacatcg
    10201 ccccgcgctt cgggtttgcc tgggcgccgt tcggaaatgg caagaccagc gttcgcggcg
    10261 cctatggcat tttctacgac tcctcgccca tgagcgccat caacaatggc atcaccgatt
    10321 cacccccgtt cgctctgaca attgattact cgccttcgcc tggaccgttc gatgaccctt
    10381 acaacgggca gaacccactg ccgatgcctt cgccgccgcc atcggatatc gatttcccgt
    10441 tacccttgtc catgtcaacg tatccgcaaa agctgcgggc ggcatacctc cagagctggc
    10501 acctgacgtt cgagcgggag ttacgccagg actggatggc acgcatcgcc tatgccggct
    10561 cgaagggcac aggtcttctt cagggatggc agtataatcc cggcatttac attcccggca
    10621 agtcgacgtt gttgaacacg aatgagcggc gccccctggc gccggaatgg tcatctatcg
    10681 gcatccgcga aggcattggc aattccaatt tcaactcgct gcaattgacg ctcgataagc
    10741 gcttctccaa gggtttcacc gctcagatga actacacctg gggaaaatcg attgatagcg
    10801 ggtcgggagc cggaacactc tggccctcct tcaataatcc gttcaatttc aggcagagcc
    10861 gcgggctgtc cgacttccat cacgagcacc gctttgtcgc gtccgggctg tgggaacttc
    10921 cgcgccttat ggggatgccc gccctggcca aaggagttct cggcgggtgg agcctcagcg
    10981 gagcgatgct gctcgagagc gggcggccgt tctcggtact cagcggccgc gacaactcaa
    11041 tgtcgggcgt cggagcggac tttgccgatc tggctggcga taccagcagg agcgcccgtc
    11101 aggatccaaa tcgcgacccg gttttggagt ggttcaacac tcaggcgttc gttcagaacg
    11161 cggagggcac attcggcaac tccggccgga acatcatttt tggtccggga cacgcgaatg
    11221 tcaatttgtc ggtatccaag agcttcccga tcgcgtttct cggggaagct tcgagaattc
    11281 agttccgcgg cgagtttttc aacctgttta accgcgtgaa cctgctgtcc aagcgctcgg
    11341 agagcctcac ttcgggcacc tacggccgga ttaccagcgc cgcggaccct cgaattctcc
    11401 aatttgcatt gaaatggcac ttctgattca cggtcctgtc atcacatgtt gtcgctcgct
    11461 tttttcgccg ttctggcact cacgatagat ggcaagctgg acgatccgtt ctggaagccg
    11521 gtgaccccgc agacgctcga gtcgccggcg ggtgggcgga tccgggcagt tgtcgcaggc
    11581 cggcaccttt atattgcggc cgaagtcccg gagcctaccg gacgcgtcac agcgcgttcc
    11641 atcggccgaa atccggcttg ggaagacgaa gacctccttc gcatctcggc gggcgcaacc
    11701 atcgggtaca cggaccgggt gctccacgtg aatccgttcg gcgcttactc tctcgagcgg
    11761 gcgggccatc ccgtctggac cagcctgagc gttttcccgt actcggatga gcgggaagca
    11821 ccggttgtgc cggtcaacat agacaagttt cttgtagcca ccagggttgg tgaaaagggt
    11881 tggaccgctg aaatcgcatt ccctttgagc gaactgagcg cgcccggtcc cgatacaatt
    11941 tacgccatga tcgagcggat ccgggcacag cggccgggag ttccgcaaca ggtctggcgt
    12001 tggcccggcc ccgggccagc cgccaggatt ccggtcgcgc cggatatccc ctgggaaacg
    12061 ccacccgccc ggttcgagcc tcctctcctc ggcaacagcg aaccgccgct cgagatcggc
    12121 agagccggca ccttgcctgc gttggagagc aagtggcagg acggagcatg ggcccatgca
    12181 cccgtctgga agttgctgct cgacgagcac cccttgcgag ctccgagact tgctaccgaa
    12241 gtaaaactcc tgcatgacgg gcgcaccctc gcggtcatgg ccaggtgcgt ggagccggac
    12301 ggcattagcg ccgaagtgaa agagaacgac ggcgcggtaa tgcaagacga cagttttcac
    12361 gtgtacctcg caacatcgcg atcggtctac gttcaggtgg tggcaaacgc gctggggtac
    12421 cttcgcgacg ccgcaggctt ttcgggcggc tcccggattt cgcggccgcg cgagtgggac
    12481 agcggaacgc gcgtccacgt ggaaagggaa gccggcgttt ggacggcccg gctcgacatt
    12541 ccgctcgaac ccgttgccga agtgctcggg gagagcacgg ttcccgcaga ctggcgggtt
    12601 cttctgatgc gcttccggcg cggccggccg ggagacgcgc gggagaccag cgttttgccc
    12661 gtcattcaga gcgataccgc gctgtgtccc gcccgctacc ggcggctgcg gcttgctgcc
    12721 aaagcgaccg caccaggcaa tacccagggg caagcggccc agacggccgg gacacctgag
    12781 ctcgcgctgg tcctcacgcg ggaacagcgc cgccagatgg gccttgccac gatgctcgac
    12841 cgccacattc gagctcgtgt gcggaaacac ctcgaagcgg accgtgacga gtggaaccag
    12901 gtgaattcgc gcggagactg ggaacgcttc cggaatccac gtctgaatgc gatgcgcgcc
    12961 tggatgggag agttcccggc ccgtacgcct cttgaagcaa gcgtcacgaa agaataccgg
    13021 gggcatgggt accggcgcca ggacctggtt tatcgcagcc ggccggggct gtgggttacc
    13081 gctaacctct atctgccgga gaagccatcc ggacaattac cgggtatcgt catcgtccac
    13141 agccaccacc ggccgagaac ccaggccgag ttgcaggaca tgggcatcct ctgggcacgt
    13201 cagggcgccg cggttctcat catggaccag atcggtcacg gagagcggat ccagacctac
    13261 ccctggaacc gggaaggtta ccgctcccgc tactccaccg gcatgcagtt gtacgtggcg
    13321 ggcgagagcc tgatcaagtg gatggtctgg gacatcatgc gcggcatcga cttgctgctc
    13381 gaacgcccgg atatcgatcg tgaaaagatt atcatgctcg gggcggtcgc gggcggtggc
    13441 gatccggcgg cggtgacggc ggctctcgat ccgcgcatcg cggccgtggc accatttact
    13501 ttcggagaat ccctgccgga gttgggtcct ggcggaagtc cctttccctc ggacctggca
    13561 gtgcccggct ggggcagttg ggaaaccacc agaaacctgc ctcacagcat cggcaagcgg
    13621 ttttttccgt ggacgatctg tgcctccgtc gctccgcggc gctttgtcta ttcctacgaa
    13681 atgggctggg aagtggagaa ggttcccgcg tgggcgcgat accgcaaggt gttcgggttc
    13741 tataacgcgt tggacaacct ggacgaagca catggcttcg ggacgtttcc cggaccgggc
    13801 gagtgcgcca acatcggacc ggcgcagcgc aagacacttt accctgaact ggagcggtgg
    13861 ttcggcatcc ccatcccgga taacgaaccg gatgaccgga ggccggaagc ggaactggcg
    13921 gcactgaccg ttgaaacggc cgcaaagctg gatatgcgga tgatccacca gctcgcatcc
    13981 gaaatcgcgg aagcgaaact gaaaacggcc cgggcggagt tggcgcgaat ggactcgacc
    14041 ggccgccgga agtggctgca acagcaatgg gccgccaagc tgggagacat tcacccgaat
    14101 ccggcgccaa aggcgattgt gcactggatg aagccttccg catccggcga agccgaaggc
    14161 atcacactcg acgtcgagtc cgggatcacc gttccgctgg tgctgctgaa agcatccact
    14221 cgcgaagcac ggcagccggt cgctatcgtc gtctcgcagg gcgggaagga acaggttctc
    14281 gcgcaccacc gggcagaaat tgaccgtctt ctggcggccg gcattgcagt ttgcctgccg
    14341 gatgtccgtg gagtgggcga aacatcgccc gatccccggc gtttcacctc cagccagaac
    14401 attagccttg cggccactga actcatgtta ggcaacaccc ttcccggcgc tcgtctgaag
    14461 gatctgcgaa ccgtcctgaa atacctccag gggcgccagg agtttgatcc tcagcgaatc
    14521 gccgtatggg gaggctccgg cgcaccggtc aacaaaggcc gcctgcttct cgatgagacg
    14581 ccagggtggc tggttggtcc ggagattcag caccaggcag agccgcttgg gggcctgctc
    14641 gccattctgg cggggcttta cgaggacagc gtgaaagcag ttgccgtgcg gcgcggactc
    14701 gctgcgtatc tcagcttgct ggaggaccgc ttcacctatg tgccctcgga tgtagtgttc
    14761 cctggaatcg cggaaatagg cgatattgcc gacttggccg cagtgttgtc tcccagacca
    14821 ctcctattgg acggactggt ggacggcagg aaccgggccg tgtctgaacc ggaactgcgc
    14881 gccacattca gccctactct ggaggcgtat cgaggccagc cggagcacct gcggttgcgc
    14941 gccacaacgg ccgaacctgg agttgcggaa tggctgatac aggtcctcgg gcgatagcgc
    15001 agcggctcta tcgagagctg ttctggatgg cctcgactaa gccatcgatg gtatagggtt
    15061 tcgcttccac ggcgacatca aggcccaact cgcgggcggt ggcggaggta acggggccta
    15121 tcgaagccag cttgaagccg cgcagcgttt cggcgcccac ggcacagacg agattcttca
    15181 ccgtcgagga actggttaca gtaatccaat cgacaggcgt ccctgacccg aacagctccc
    15241 gggcgcgggg cacaacgctc tccggcatgg tggtccggta ggcctccacc acgtcgacct
    15301 gtgcgcccat tcgcgatagc tccgccggaa ccaggtcccg cgccacagcc gctctcggca
    15361 atagaatacg tttgcctgcc acgtccgcgg ttgccagcga ggccacaagg ctttccgcca
    15421 cgtactcctc cggtgtcagg tccactttca aatggagttc ctcgacagcc cgacgtgttg
    15481 ccgggccgat ggtgcagatc cgcgcgcgca gcgatctcag gtccactcgc gagcggtcca
    15541 gggcctcgag aaaaaatcgc actccgttta cgctggtgaa cagaatccag tcgtaatcgc
    15601 cgagatgcgc gatgcccgcg tccagaggac cggagtccgc gggagggccg atctgaatcg
    15661 ccggaagctc gcggacacaa gcgccgagag accgaagcct ggccgaaaga tcgcccgctt
    15721 gagacgccgc gcgcgtcaca acaatcgtct ggccgaatag gggaagccgt tcaaaccagt
    15781 tcagccgcgc accgagttgt accacttcgc ccacgacgat cgtggccggc ggtttcatgc
    15841 gccgctcctc tattaagcgc ggcagagtgg cgatcgtccc ggcgatcctt acctggtccg
    15901 ggcgggtagc ccaacgaacc gccatcgccg gagtgtcggg cgagcggccg tttgcaatga
    15961 ggttccgggc gatatcgggg aaagaggtca accccatgta gatcaccagc gtctccgcct
    16021 ggcccacttt cgtccagtct atggcgtcca cattatgccc gctgacaaag cttacagccg
    16081 aggtgtgccc gcgatgtgtc agaggcactc ccgtataagc ggcgattccc agcgcggtag
    16141 ttacgccggg caccacttcg aacgggacac cggcttccgc gagcgcctca gcctcttccc
    16201 ctccccggcc gaagatgaac gggtcgcctc ccttcagccg gacgacaacg tggccgggac
    16261 gggctttctc caggagcaat gcgcagatct cttcctgggt gatattatgc gccgctttct
    16321 ttttcccgac ataaatgcgc tccgctgttt cgggggcgag atcgagcaac atcgcattgg
    16381 ccaggttgtc gtacagcacg gtgctcgcct tgctgaggat ctcccggccc ttgacaggga
    16441 taaggtccgg atcgccggga ccggcgccta ccagaaaaac tttaccgctt gctgacgtgc
    16501 gcggctccgt ttccgcgata tcggtttctg agccgcgacc gtgagggagc ggtttcttag
    16561 ggtcctgagg ttccgtatac aacctccagg atggcgcgcg cgcccgcatc gagaatatcc
    16621 ctgcccaatt cggcgccgac gtgctcagcc tccgccaccg cgccggaatt ctcccgccgc
    16681 acgatttcgc ggccatcggg cgagatgacg acggccctca gatggacgcg gccgccttcg
    16741 acaacggcat gtcctccgat cggtacctgg cagccgccgc cgagtgatcg caatagtgcg
    16801 cgctcggccg tgacggctgc cctggtctgg gcgtgttcga gtttgcggca agcctggcag
    16861 gcatgtcctt cgtccgctct ggtctcaatc gcgagcgcgc cctggcccac ggcggggcac
    16921 atcacttccg gcggaagcgg ttcggcgatg cggccttccc atcccattct tttaagacct
    16981 gccgccgcaa gaacaatgcc cgcatactgc ccttcgtcga gcttgcgcag ccgcgtatcg
    17041 atattgccgc gcagcggctc aataaccaga tccggccggc aggctcgcag ttgcgcggcg
    17101 cggcgcaggg aactggtgcc taccttcgcg tttcgcggca accgtgctag cgtactgccc
    17161 accaggacat cccgcgcatc ctcccgttca ggaaccgctg ccagcacaag accgtccggc
    17221 aaatccgtcg gcatgtcttt cagactgtgc acggccagat cgatgcggcc gccgagcagc
    17281 gcctcttcga tctcttttgt gaacagcccc tttacacccg tgctggcccc caggcgcgcc
    17341 agcgacacgt ccgtaatgtt atccccggta gtccggatga tctcgatttc gcattgttcg
    17401 ccctgacgct caagagcgga atacacccac tgggcctgcc agagtgcgag cttcgagccg
    17461 cgtgagccga tcctcagcat
//
LOCUS       DASTMK010000284         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_365796, whole genome shotgun sequence.
ACCESSION   DASTMK010000284 DASTMK010000000
VERSION     DASTMK010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365796"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFQ79_12260"
     CDS             complement(<1..478)
                     /locus_tag="VFQ79_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU0140485.1"
                     /translation="MGQPFAVKRVEEIDLPQCEKGIGKVIQSPDSPDLIAGVRVQPYA
                     LWPDDRGYFLEVIRMGKGLVSHYSPETTQVSTALSYPGTIKAFHYHQHQTDFWVPAAG
                     MLQVALVDLRSDSHTFGARNTLYLGALRPWQVLIPPGVGHGYKVIGDSPAVLVYVTN"
     gene            complement(478..1242)
                     /locus_tag="VFQ79_12265"
     CDS             complement(478..1242)
                     /locus_tag="VFQ79_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0140486.1"
                     /translation="MKGIILAGGTGSRLFPLTKITNKHLLPIYDKPMIFYPLQSLVDA
                     GIQDILVVTGGKNSGDFLRLLGNGKEFGLKHLNYTYQEGEGGIADALSLAEHFADGQR
                     ICVILGDNIIEGSIRPAVENFRRQERGARILLKKVPDAERFGVAEIDGNRIVGIEEKP
                     LKPKSNYAVTGIYLYDETVFDKVKQLVPSNRGELEITDVNNAYIREQSMSFDYLEGWW
                     TDAGTFDSLLRAANLVKESRETMEDVAASVLGGAEN"
     gene            1367..>2234
                     /locus_tag="VFQ79_12270"
     CDS             1367..>2234
                     /locus_tag="VFQ79_12270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140487.1"
                     /translation="MIRGSILASVLVLGTAATGFAQQITSVVNFASLDNRLSPGALAF
                     VRGTGLNIGSPPAVTVTQGVARAAWVSPNDVSATQLLIQIPLDATAGPGTLTVGAASY
                     PINVTPYAPGVFSRDSTGTGIGSFLHTTGGQIVSLTTPAAPNESLRVFAIGLGQTNPA
                     GTTGVPFPGVDVPTAATPVVRVGGVPATGTSSVLVAGSVGFYRITFNVPSGLPKGRYD
                     VIVSMGGQESNAVTLPVTGPFAIPDFDENDVPDLIWQNDGTRQVSVNYYGGTGGATMI
                     GWNWLHAGGAPGW"
BASE COUNT          435 a          626 c          692 g          481 t
ORIGIN      
        1 ggttcgtcac atagaccaga accgcggggc tgtcgccgat caccttgtag ccgtgtccaa
       61 cacctggagg aatcagcact tgccatggcc gcagcgcgcc caggtacagt gtattgcgcg
      121 cgccgaacgt gtgcgaatcg ctgcgaaggt cgaccagcgc cacctgtagc attcccgccg
      181 cgggtaccca gaagtccgtc tgatgctgat gatagtggaa agccttgatg gttccgggat
      241 agctgagggc ggtggagacc tgcgtggttt cgggcgaata atgcgagacc aggcctttgc
      301 ccatgcggat cacttccaga aagtacccgc gatcgtcggg ccacagagca tagggctgca
      361 cacgcacacc cgcgatcagg tccggagaat ccggcgattg aatgactttt ccgattcctt
      421 tttcacattg cgggagatcg atttcctcaa cccgcttcac ggcgaatggc tggcccatca
      481 attctccgcg ccgcccagga cggaagctgc aacgtcctcc atggtctcgc ggctctcctt
      541 caccaggttg gccgcccgca ggagcgagtc gaaagttccc gcatcggtcc accagccttc
      601 gagatagtcg aacgacatgc tttgttcccg gatgtaggcg ttgttcacgt ccgtgatctc
      661 gagttcgccg cggtttgagg gaacgagttg tttgacttta tcgaacacgg tttcgtcgta
      721 caggtaaatg ccggtgaccg cgtaattcga cttgggcttg agaggttttt cttcgatgcc
      781 tacgatccgg ttgccgtcaa tttcagcgac gccgaaacgc tcggcgtccg gaactttctt
      841 cagcagaatc ctggcgccgc gctcctgccg ccggaagttt tcgacggccg ggcggatgct
      901 gccctcgatg atgttgtcgc ccaggatgac gcagatgcgc tggccgtcgg cgaaatgttc
      961 ggccagagag agcgcgtcgg cgatgccgcc ctcgccttcc tgatacgtat agttcaggtg
     1021 cttcaatccg aactctttgc cgttccccag cagacgaagg aaatcgcccg agttcttgcc
     1081 gccggtgacc accagaatgt cctgaatccc cgcatcgacg agtgattgca aaggatagaa
     1141 gatcatcggc ttgtcataga tggggagcag gtgcttattg gtaattttcg tcagggggaa
     1201 aaggcggctg ccggtgcctc cggccaggat gatgcctttc atcgcggtgc ctccgagtac
     1261 aacaacccat catgatacat gggatctagt actggagtcg ggctggaaac cgactacaat
     1321 accaaaggta agtcgaaata gaaccgcttt aatcggagga atcaatatga ttcgaggctc
     1381 tattctggcg tcggtgctcg tgttgggaac cgcggcaacg gggttcgcac agcagattac
     1441 gtcggtagtg aatttcgctt cccttgataa ccggctttcg ccgggcgcac tggcgtttgt
     1501 gcgtgggaca gggttgaaca tcggctcgcc gccggccgta acggtgacgc agggagtagc
     1561 cagggcggcc tgggtttctc cgaatgacgt ttccgcgacc cagcttctga ttcagatacc
     1621 gctcgatgcg acagccggcc cgggaacgct cacagttggc gcggcatcct atcccatcaa
     1681 tgtgactcca tacgctccgg gcgtgttttc gagagactcg accggaacag gcatcggttc
     1741 gtttctgcac acgactggtg ggcagattgt gagcctgacg acaccggccg ctccgaacga
     1801 atcgctccgt gttttcgcga taggcttggg ccaaacgaat cctgccggta ccacaggcgt
     1861 gccctttccg ggagtagatg tgcccacggc ggccacacct gttgtccggg tcggaggtgt
     1921 accggccacg gggacatcct ccgtgctggt ggcgggatcg gtggggtttt atcggattac
     1981 gtttaacgtt cccagcggct tgccaaaagg ccggtatgac gtgattgtgt ccatgggagg
     2041 gcaggagagc aacgcggtga cgctgccggt gaccggaccg ttcgcaatcc ccgatttcga
     2101 cgagaacgat gtgccggact tgatttggca gaacgatggg acgcggcaag tgagtgtgaa
     2161 ctattacggc ggaacgggtg gggcaacgat gatcggctgg aactggctgc atgccggtgg
     2221 cgcacctggg tggc
//
LOCUS       DASTMK010000285         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_269821, whole genome shotgun sequence.
ACCESSION   DASTMK010000285 DASTMK010000000
VERSION     DASTMK010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269821"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..2750)
                     /gene="glnD"
                     /locus_tag="VFQ79_12275"
     CDS             complement(228..2750)
                     /gene="glnD"
                     /locus_tag="VFQ79_12275"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEU0140488.1"
                     /translation="MEGRAAVARGDLRMPGRLPHLRQAFFETGDAARVLAGRTSFIDD
                     IILKLYNDVLAPVFPSGLVLLAAGGYGRCELFPCSDVDLLILSEQDLELPRSRESLSA
                     FLRQLWDLGLRLSHSIHTPGECLEIQSHNVELNISLLDQRHLAGDLELHARLERRFPG
                     FLRAQRNTLSRHLVRLAGERHARHGQTTYHLEPDIKEGPGGLRDLHLLSWLRRLRNAG
                     DPYPTRESTAEELGKQIEDAREFIHTARCFLHYQAGRDNNRLTFASQDAIAEQPFTRQ
                     RDPALWMRDYYRHASSVFKAAVRAMEAAGGTEGSLLTQFRDWRSRLSNADFTVSRERV
                     YLRSPHQLHPDPDLVLRLFQFVARHGLRLALDTERRLERALPSLTGYYSRSRPLWPAI
                     EELLNLPHAHMALRMMNDTGVLRALFPEWEAIEHLVVRDFYHRYTVDEHSLLAIQTLA
                     ELKTGGEAPRRGFANLMLEIESTAPLYFALLFHDTGKGRGLGTHVAESVRAADDAMER
                     IQVPAGVRKLVRVLIRHHLDLSAAMSSRDLDDPDVIQDLAARIETIEQLKHLALLTYA
                     DISAVNPGAMTPWRREQLWKAYLRTYRELTTELEDNRISAFSSTAPDETAFLEGFPTR
                     YLRTHTPPEIREHLELRKEAASTGVALRVSKSDSGYSLVIVARDRPFLFASIAGALAG
                     FGMNILKAEAFANRNGEVLDTFVFADPLRTLELNPVEVDRLRLTIERAVLDRLDVRRL
                     LDRPNPPAPSRSAQVKPSVSFDSTASATATLVEIVAADRPGLLYALAHCISDAGCDID
                     VVLVDTKAHRALDVFYVTSELGKLTDNQQAQLRDRLLKACEV"
     gene            complement(2750..>2830)
                     /locus_tag="VFQ79_12280"
     CDS             complement(2750..>2830)
                     /locus_tag="VFQ79_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU0140489.1"
                     /translation="GDGKIFVYDVAEAIRIRNNDRGELAL"
BASE COUNT          580 a          840 c          865 g          545 t
ORIGIN      
        1 taccccgttg ggcgcaagat cacagatgaa gagttcgtgc aaatcaatct gcagcccgac
       61 acattccatg gagaatggaa ctacacgatt cttccaagcg agaaccacca gttgtaaagt
      121 ttatttattt acgtctccta agtacacggt ttcgcaaata tgatcacgta ttctccgtcc
      181 ctatgaaaac ggctcccctg cggtcgcgga gcggttgtgc gtcacaatca cacttcgcac
      241 gctttgagga gccggtctct caactgcgcc tgctggttgt ctgtcagttt ccccagctcc
      301 gacgtgacgt agaacacgtc cagtgcgcga tgggctttcg tgtccaccag aacgacgtct
      361 atgtcacagc cggcatcgga aatgcagtgc gccagagcat aaagcaatcc gggccggtct
      421 gctgctacga tctctaccag cgttgccgtc gctgaggccg tactgtcaaa gctgacggac
      481 ggtttcacct gggcgctccg ggacggggca ggcggattcg gccggtctaa aaggcgccgg
      541 acatccaggc ggtccagcac cgctctctcg atggtcaacc ggagacgatc cacctcaacc
      601 gggttcaact ccagcgtccg aagcgggtcc gcgaacacaa aggtatcgag cacctcgccg
      661 tttctgttgg cgaaggcctc ggccttgagg atgttcattc caaagccggc gagcgcgccg
      721 gcgatggagg cgaacagaaa cgggcggtcc ctcgcgacaa tcaccagaga atagccgctg
      781 tcgctctttg aaacacgcaa cgctacaccc gtggaggcgg cctctttccg aagctcgaga
      841 tgttcgcgga tttccggcgg cgtgtgggtg cgaagatatc gggtcgggaa tccttcgagg
      901 aaggcggttt cgtccggtgc agtggaagag aaggccgaga ttctgttgtc ttccagctcc
      961 gtggtcagtt cgcggtacgt gcgcaaatag gctttccata attgctcacg ccgccatgga
     1021 gtcatggcac ccggattcac cgcgctgata tctgcgtagg tcaacagggc aagatgcttg
     1081 agttgttcga tcgtctcgat gcgcgccgca agatcctgta tcacgtccgg gtcgtccagg
     1141 tctctcgacg acatggctgc cgaaagatcc agatggtgac gtatcaacac ccgtacaagc
     1201 ttgcgcactc cagccggtac ctggatgcgt tccatcgcgt catcagcggc tcgaacggat
     1261 tcggcgacgt gcgttcccaa acccctgccc ttgcccgtat cgtgaaaaag cagcgcgaag
     1321 tatagaggcg cggtcgactc aatctccagc atcaggtttg cgaaaccgcg gcgcggtgct
     1381 tctccgccgg ttttcagttc cgccaaagtt tggatggcca gcagcgaatg ctcgtccacc
     1441 gtgtaccgat ggtagaaatc gcgcactacg agatgttcaa tcgcctccca ttccgggaac
     1501 aatgcgcgca gcaccccggt gtcgttcatc atgcggagcg ccatatgcgc gtggggaaga
     1561 ttcaacagct cttcgatagc gggccacaac gggcggctcc gtgaataata gccggtcagc
     1621 gaagggaggg cgcgctcgag ccggcgctcc gtatccaggg ccagccgaag gccgtggcgc
     1681 gcgacgaact gaaacaatcg aagcaccagg tccggatcgg gatggagctg gtgtggagac
     1741 cgcaaataga cgcgctcgcg ggatacagtg aagtcggcgt tcgacaagcg ggaccgccag
     1801 tcgcgaaact gggtcagtaa cgacccttcc gttcctccgg cggcttccat ggcccgcacc
     1861 gccgccttga agaccgagct tgcatgccgg tagtagtcgc gcatccacaa cgcggggtcc
     1921 cgctggcggg taaagggctg ttcggcgatg gcatcctgcg atgcaaacgt caggcgattg
     1981 ttatcgcggc cggcctggta gtgaagaaag cagcgcgcgg tgtgaatgaa ctcacgcgca
     2041 tcctcgatct gcttcccgag ttcctctgcc gttgattccc gggtgggata gggatctccc
     2101 gcgttgcgca agcggcggag ccaggataac agatgcaggt cccggagccc acccggtccc
     2161 tctttaatgt ccggttccag gtgataggta gtctggccgt gtctggcgtg ccgctcaccg
     2221 gcgagacgga ccaggtggcg cgataacgtg ttgcgttgcg cgcggagaaa acccgggaag
     2281 cgtctttcga gccgggcgtg cagttcgagg tctccggcca aatgccgctg gtccagcagg
     2341 ctgatattca gctccacgtt gtgggattgt atttcgagac actcgccggg ggtgtggatc
     2401 gaatggctca gccgtagtcc caagtcccac agttgccgga gaaaggcaga caggctctcg
     2461 cgagagcgcg gcagttcaag gtcctgctcg gacaggatca gcaaatcgac gtcggaacac
     2521 ggaaacaact cacaccggcc gtatccaccg gcggcaagca atacaaggcc ggaggggaac
     2581 accggggcga ggacatcgtt gtagagctta aggatgatgt cgtcgatgaa cgaggtccgg
     2641 cccgccaata cccgtgccgc gtcgccggtc tcaaagaacg cctggcgaag gtgcggcaaa
     2701 cggccgggca ttcgcaggtc gcctcgcgcc acggcagccc ggccttccat cacagggcca
     2761 attcgccgcg gtcgttgttg cggatccgga tcgcctcggc gacatcgtac acaaagatct
     2821 ttccgtctcc
//
LOCUS       DASTMK010000286         4465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_622012, whole genome shotgun sequence.
ACCESSION   DASTMK010000286 DASTMK010000000
VERSION     DASTMK010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622012"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="ahcY"
                     /locus_tag="VFQ79_12285"
     CDS             complement(<1..1027)
                     /gene="ahcY"
                     /locus_tag="VFQ79_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU0140490.1"
                     /translation="MATNAEAPFACDVKDLDLAGLGKRRIEWAFQSMPVLQSIRKQFI
                     KTQPLDGIRIAACLHVTTETANLMATLRDGGASVALCASNPLSTQDDVAASLVRDYNI
                     PVFAIKGEDNDVYYSHITSALDLKPHITMDDGADLVSVLHTKRQELLDNVIGGTEETT
                     TGVIRLKAMANDGVLRYPIVAINDAMTKHLFDNRYGTGQSTIDGILRSTNVLLAGLNF
                     VVAGYGWCGRGVAMRARGLGANVIVTEVDPIKAIEALMDGYRVMSMHEAASIGDVFVT
                     VTGDKGVLAKDHFEKLKNGAILCNSGHFNVEIDLEALEKFSSSRRQAREFVEEYSMRD
                     GRKIYVLG"
     gene            complement(1082..2254)
                     /gene="metK"
                     /locus_tag="VFQ79_12290"
     CDS             complement(1082..2254)
                     /gene="metK"
                     /locus_tag="VFQ79_12290"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU0140491.1"
                     /translation="MAGPQRQIFTSESVTEGHPDKIADQISDGVLDAVQKDDSTGRVA
                     CEVLVTTGMCIVAGEITTNTYIDVPKLARSIINDIGYNDARMGYDSNTCGVLNMIQSQ
                     SPDIAMGVDTGGAGDQGLMFGYACEETEELMPLPIMLAHKLVRRLSDVRREGLLGFLR
                     PDGKSQVSVEYVDGMPKRIDAVVVSTQHGDDVSTETLRAEVKKHIIDPVVPGNMVDSG
                     TAYHINPTGRFVIGGPHGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSACYM
                     ARYIAKNLVAAGLASRCEVQLAYAIGVAEPVSVTVNTFGTGAVEEGRLTDLVRTHFPL
                     TPREMIDHLNLRRPIYRKTAAFGHFGRTEDTFTWEAADKAEALREDAKLAGAASRG"
     gene            2434..3798
                     /locus_tag="VFQ79_12295"
     CDS             2434..3798
                     /locus_tag="VFQ79_12295"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HEU0140492.1"
                     /translation="MHSFRALFPVLFTAFLSQGAVPPKPITLNDDGAWCWFQDERAVV
                     LGGTLIAGSIAAGTRDAARRGDVEVVTFEFATGRQTRTELHDRLLTSREVYDDHNAPA
                     FFIRPDRRILAVYSMHGPENRFYYRISERDDPTRWSPEKHFIPTPGSRITYSNLHFLA
                     KENGGKGRLYNFYRGLDNSFKPSYAFSDDLGETWQSGNIVIDVPSGFRHRPYAKYASN
                     GQDTIHLFYTEAHPRDFDNSTYHVFYKDGNLHRSGGSLLRSLKEGLRKPEEGTRIFQG
                     DPDNVAWVSDIHLDSAGHPYVAYTVQKDSAGLPGGKGGMDLRYRYARWTGTEWRDYEI
                     AHAGSRLYPGEDDYTGNIALHPDDPDSVVISTNADPVSGKPLVSSKDNQRHYELFRGV
                     TKDGGRTWKWTALTPDPVEDHLRPIIPHAPGGRTALLWLKGVYRTYTNYDLSVMLLYL
                     AGRP"
     gene            complement(3777..>4465)
                     /locus_tag="VFQ79_12300"
     CDS             complement(3777..>4465)
                     /locus_tag="VFQ79_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEU0140493.1"
                     /translation="YEEFGVEDPRVTQLEEEYVITYSAYSRNGVRIALAKTKDFRRVE
                     RVSLITQADYRNVVIFPEKFDGLYARLDRPHSEIAPWSIWISYSPDLRFWGESQLIMR
                     PVPYHWDEMKIGPGAPPIRTDKGWLSIYHGVFETMDGSVYRLGVALHDLGDPAKILGV
                     SDSWILQPEDPWEVAGYVHNVVFTCGAVPEPDGTVKIYWGGADTVMCVGEADLSSLVD
                     LCLDHGRPAR"
BASE COUNT          874 a         1429 c         1284 g          878 t
ORIGIN      
        1 cgccgagcac atagatctta cggccgtcgc gcatcgaata ctcttccacg aactcacgcg
       61 cctggcgccg ggaggaggag aacttttcca gcgcttcgag atcgatctcc acgttgaaat
      121 ggccggagtt gcagagaatc gcgccgttct tcagcttctc aaaatgatct ttcgccagca
      181 cgcccttgtc acccgtaacg gtgacgaaca cgtctccgat ggaagccgcc tcgtgcatcg
      241 acatcacccg gtagccatcc atcagcgctt caatcgcttt gatcgggtcg acttccgtca
      301 cgatcacgtt ggcgccgagc ccccgggcac gcatcgccac gccacgcccg caccagccat
      361 agccggcgac gacgaaattc aaaccggcaa gcagcacgtt cgtcgagcgc agaatcccgt
      421 cgatggtgct ctggcccgtt ccataccggt tgtcgaaaag atgcttcgtc atcgcatcgt
      481 tgatggcgac gatggggtag cggaggacgc cgtcattggc catcgccttc aggcggatga
      541 cgccggtggt cgtttcttcc gttcccccga tgacgttatc cagcaattcc tggcgcttgg
      601 tatggagcac gctgaccagg tccgcgccgt cgtccatcgt gatgtgcggc ttcaggtcga
      661 gagcggacgt gatgtgggag taatagacgt cgttgtcttc ccccttgatc gcgaacaccg
      721 ggatgttgta atcccgcacc agcgatgccg ccacgtcgtc ctgcgtggaa agcggattgg
      781 acgcgcacag cgcgaccgaa gcgccgccgt cccgaagggt cgccatcagg ttggcggtct
      841 ctgtggttac atgcaggcag gcggcgatcc gaatgccgtc gagcggctgc gttttgatga
      901 attgcttgcg gatggactgc aaaaccggca tcgactggaa cgcccactcg atacgccgct
      961 tgcccaggcc ggcaaggtcc agatctttca catcacaagc aaaaggagct tcagcattag
     1021 ttgccatgaa aacctctgat ctttgagcgc ccgccggaga cgttcggccg ggctgggaat
     1081 atcaaccccg gctggccgct ccggccagtt tcgcgtcttc gcgcagggct tccgccttgt
     1141 cggcagcttc ccaggtgaac gtgtcttcgg tgcgtccgaa atggccgaaa gccgcggtct
     1201 tacggtaaat cggccggcgc aggttcaggt gatcgatcat ctcgcgcggc gtcaacggga
     1261 aatgcgtccg gaccaggtct gtcaggcgtc cctcttccac cgcgccggtt ccgaaagtgt
     1321 tcaccgttac cgagacgggt tccgcgacgc cgattgcgta tgccagttgc acctcacagc
     1381 gggaggcgag cccggcggca accaggttct tcgcaatata gcgcgccatg tagcaggccg
     1441 agcggtccac cttcgtcggg tcttttcccg agaaagcgcc gccgccgtgc cgccccatgc
     1501 cgccgtacgt gtcgacgatg atcttacggc ccgtcagacc ggtgtccccg tgcggcccgc
     1561 cgatgacgaa gcgcccggtg ggattgatgt gataagcggt tccggaatcg accatgttgc
     1621 ccggaacgac cgggtcgata atgtgcttct tcacttcagc ccgcaaggtc tcggtggaga
     1681 catcgtcgcc gtgctgcgtc gagactacaa ccgcatcgat ccgcttcggc atcccgtcca
     1741 cgtactccac gctgacctgc gacttgccgt cggggcgcag aaagccgagc aacccttccc
     1801 gtcttacatc gctcaaccgg cgaaccagct tatgcgccag catgatggga agcggcatca
     1861 actcctcggt ctcttcgcag gcatacccga acatcagtcc ctggtcgccg gcgccgcccg
     1921 tatccacacc catcgcgata tcgggagact ggctctgaat catgttcaga accccgcagg
     1981 tgttgctgtc gtaacccatg cgcgcgtcgt tgtagccgat gtcgttgatg atcgagcggg
     2041 ccaacttggg cacgtcgata taggtgttcg tcgtgatttc gccggcgaca atgcacatgc
     2101 cggttgttac cagcacctcg caggcgacgc gcccggtgga atcgtctttc tgaacagcgt
     2161 ccagtacgcc gtcggaaatc tggtctgcaa tcttgtcggg gtgcccttcc gttacggact
     2221 ccgaggtgaa aatctgccgt tgtggcccag ccatcgttag ccttgctcct tgcgaacttc
     2281 gagttggcgg gtgtttccgt tccatcgatt gtacggaaca cccgagggaa aagccgaatg
     2341 ttttagtcta gcgtcaagga aacgggcgag tcaatgccgg gctgttaaga tggaaggcgt
     2401 tgccgcagcc agaaccgatt gaaaggtaaa gacatgcatt catttcgcgc actcttcccg
     2461 gtactcttta cagcctttct cagccagggc gccgttcctc cgaaacccat caccctcaac
     2521 gacgacggag cgtggtgctg gtttcaggac gagcgcgctg tcgttctggg aggcacactg
     2581 atcgccggtt ccatcgccgc cggcacgcgt gatgccgcgc ggcgcggaga cgtggaggtc
     2641 gtcacctttg aattcgccac cggccgccag acccgaactg aattgcacga ccgcctgctg
     2701 acttcgcgcg aggtctacga cgaccacaat gctcccgctt tcttcatccg tcccgatcgc
     2761 cgcatcctgg ccgtgtattc catgcacggt ccggaaaacc gcttttatta ccggatttcc
     2821 gagcgggacg accctacccg ctggagtccg gaaaagcatt tcatacccac cccgggctcg
     2881 cgcattactt attccaacct gcatttcctc gcgaaggaga acggcggcaa agggcgtctc
     2941 tacaacttct atcgcgggct ggacaacagc ttcaaacctt cgtatgcgtt ctccgacgat
     3001 ctcggcgaga cctggcagtc cggcaacatc gtcatcgacg ttccttccgg gtttcgtcac
     3061 cgcccgtatg cgaagtacgc ttcgaacggc caggacacta tccacctgtt ctacaccgaa
     3121 gcacacccgc gcgacttcga taacagcacc taccatgttt tctataagga cgggaatctt
     3181 catcgctccg gcgggtcgct gctccgaagt ctgaaggaag ggctgaggaa gccggaggaa
     3241 gggactcgaa tcttccaggg cgaccccgac aatgtcgcgt gggtgagcga catccacctg
     3301 gatagcgcgg gacacccgta cgttgcctat acggtgcaga aggattccgc cggccttccg
     3361 ggcggcaaag gcggcatgga tttgcgatac cggtacgcca ggtggaccgg aaccgaatgg
     3421 cgggattacg agatcgcaca cgccggcagc cgtctgtacc ccggcgaaga cgactatacc
     3481 ggcaacatcg cactacaccc ggacgacccc gacagcgtcg tgatctcgac gaacgccgac
     3541 ccggtttcgg gcaagccgct cgtcagctca aaagataacc agcgccacta cgagctgttc
     3601 cggggcgtca ccaaagacgg cggccgcacg tggaaatgga ccgcgctcac accggacccc
     3661 gttgaagatc acctccgccc gatcattccg cacgcgccgg gaggccggac agcgcttctg
     3721 tggctgaagg gcgtctaccg cacctatacc aattacgatc tctcggtgat gctgctctac
     3781 ctggccggcc ggccgtgatc gaggcagagg tccaccaggc tcgagagatc ggcttcgccc
     3841 acgcacatca ccgtatcggc gccgccccaa tagattttga cggttccatc gggctccggc
     3901 acggctccgc aggtgaaaac cacgttgtga acgtagccgg cgacctccca gggatcttcc
     3961 ggttgcagaa tccaggagtc gcttacgccc aggatcttcg cgggatcgcc gaggtcatga
     4021 agagcgacgc ccaggcggta gacggacccg tccatcgttt caaaaacccc gtggtagatg
     4081 gacaaccatc ccttgtccgt cctgatcggg ggtgcgcccg ggccgatctt catctcatcc
     4141 cagtgatagg gcacgggcct catgatgagc tgggattcac cccaaaaacg caggtcggga
     4201 gaataggaga tccagatcga ccacggagcg atctccgaat gggggcggtc cagccgggca
     4261 tacagcccat caaacttttc cgggaaaatc accacgttcc gatagtctgc ctgcgtgatg
     4321 agggacactc gctccacccg gcgaaaatcc ttcgtcttcg ccagggcgat ccgcacaccg
     4381 ttgcgcgagt acgcgctata agtgatgacg tactcctctt cgagctgcgt cacgcgcggg
     4441 tcttccacac cgaattcctc gtagc
//
LOCUS       DASTMK010000287        11714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_365852, whole genome shotgun sequence.
ACCESSION   DASTMK010000287 DASTMK010000000
VERSION     DASTMK010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365852"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFQ79_12305"
     CDS             <1..204
                     /locus_tag="VFQ79_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0140494.1"
                     /translation="HSFATHLVEGGADLPTVQAIPGHADLKPTSIYLHMSERHLRAAG
                     TPIDKAELTNLDQVKRSRKLHKK"
     gene            444..1049
                     /locus_tag="VFQ79_12310"
     CDS             444..1049
                     /locus_tag="VFQ79_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140495.1"
                     /translation="MDTCRLDELERRTYRTVIDDGLGDIVLGIFFVALGLGIATGLGW
                     LGALAGAVAVPAWRGLRKTFVEARTGYVRLNRSRRAQLRRGPFIVATIAVLIPLGLVG
                     VEGLLGREFGKIPGLVPVLVGVGIGIPVAAAGYLFEIRRLSVYAGLIWLALAVAYVGR
                     IPYGWVIFAFGVVVMGSGLFYLASFMRRYPAKPNGVSADDY"
     gene            1039..2526
                     /locus_tag="VFQ79_12315"
     CDS             1039..2526
                     /locus_tag="VFQ79_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU0140496.1"
                     /translation="MTTEAISSPGTSPSHIDRIVHEPGRLKILSQLYVVESADFLFIM
                     QHTGLTQGNLSSHISKLEAAGYVEVQKEFVKKRPRTLLRMTRLGREALEVYVTGMKRL
                     LEDVPVPGPAAVEKSRGARRRSRASLGICLVALLASVANARTTEVRFNSYDGYPMLGK
                     LTMPETRGPHPVVIYVQTAEGMTADMKRPGRDGTFSYFDLYRTKLAELNVGFFAYEGR
                     GIRGGDSPPRYEAIDWPVYNTSTLENKVRDVLSAVQVVRKQRGVKLSRIFLMGASEGT
                     LLAAEAASRAPLQIRGLVLYGVMATTMRDAFKFMVSDGGFLAYRGFFDTDKDGMISKQ
                     EFEIDPQRYRERALRNADFEKFDHNRDGLFTVDEMRLLSKPYLDAVDSDDYQILNRWA
                     RTAAGVSTPDNWFKDHFEHAPIWTFLSRLDMPIGLFHGEADTQLPVGPLRQLEASAKK
                     ARKSRMMFHYFEGLDHSLDIRQYFARGILPAGHKAIFEYISRHAR"
     gene            2678..3298
                     /locus_tag="VFQ79_12320"
     CDS             2678..3298
                     /locus_tag="VFQ79_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0140497.1"
                     /translation="MKQIYKRLAVMMALVAAPALPASISLDFTGPLEALPGQSLSLLG
                     GSIVVTATRGGQEADITWREGRGLGVAGGQSDEIDWSGKSEKLIFSLTNPGASLYLTG
                     FTLNSLYADEFLWFDEKGQVTISRNGFEDLTFTFAGIERKGDGHLYVPLPTGVQFDRL
                     TFSTQGPSLLSDFAVGGIDVATPEPATYALMGAGLLALGAIRRRSS"
     gene            complement(3304..4290)
                     /locus_tag="VFQ79_12325"
     CDS             complement(3304..4290)
                     /locus_tag="VFQ79_12325"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU0140498.1"
                     /translation="MIAIPAHLSPADLVEPGPSETRREFSGETMGTTWSAVLYAAQTL
                     PDELFLRRIQWTLEGVVSQMSHWRPDSALSEYNRAPAGRWQVLPDEFRCVLESALEIA
                     RLSEGAFDPSLGEAADQWGFGPSGPRRSPPPAAELAPPLHDWRHIELDPKAGRALQPG
                     GVRLDFSSIAKGFAVDLLAALLEQLGASSYLVEIGGELRARGVKAGGQPWWVWIEPPP
                     GCPLKAKVALCGKAVATSGDYRFRFEHEGRSYAHTLDPRTRAPLTSPPSSVTVVYDRC
                     MDADAWSTALMVLGVDSGLAMCARHEIAALFLVRENAGWRAVASPAFGAMLV"
     gene            complement(4287..5162)
                     /locus_tag="VFQ79_12330"
     CDS             complement(4287..5162)
                     /locus_tag="VFQ79_12330"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEU0140499.1"
                     /translation="MKLPSFPAFAALAVIALPATLLAHRQWMLPSATVLSKTDTWITV
                     DAAVSNELFFFDHVPMRVNNVVITCPDGSTIQPQNVATGKYRSTFDVHLQQPGTYRIA
                     NVNNMVFASWSENGQPKIWRGTAAEAASAVPQQAQDLKIARMNNRIELFVTAGKPDTK
                     ALQPSGADLELVPITHPNDLVAGEEATFQFLHNGKPVADLEVMVIPGGIRYRDQLHEQ
                     TVKTGSDGKFVVRFAQPGMYWLNATVGAARRGPGGPGGPGGPGGPGGPGGPGQMPMPA
                     GDRANYVVTLEVLPQ"
     gene            complement(5201..5728)
                     /locus_tag="VFQ79_12335"
     CDS             complement(5201..5728)
                     /locus_tag="VFQ79_12335"
                     /inference="COORDINATES: protein motif:HMM:NF021540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEU0140500.1"
                     /translation="MKTLLPSAALTGLLVSPVLTGAELEITVEIPQIQVAEYHRPYAA
                     IWIERADQSVAADLAVWYMLKSSAKGEQGDTWLKDLRTWWRKSGRSQTLPIDGVTGAT
                     RPPGVHKLSFKDSGPQLASLAPGAYHVVVEVAREVGGRELVRVPFQWPASKAPATAKA
                     QGSHEIGGVQVTVKP"
     gene            complement(5748..6470)
                     /locus_tag="VFQ79_12340"
     CDS             complement(5748..6470)
                     /locus_tag="VFQ79_12340"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEU0140501.1"
                     /translation="MPYASIRKPARQETAPLTPAGKVNHPAAPPRPSVPKRSFRAALV
                     RQVTLWHWVSSGVTLGGMLLFTITGITLNHASQIPAEPVVRVREAAAPAPVAAALVPA
                     PDEQEGRRPLPAAVATWLAREFGSAGDGTGEWSRDELYVSLPRPGGDAWVTVDRSTGL
                     AHFEQTDRGWIAYFNDLHKGRHTGHAWSIYIDVLAVACLVFSFSGLILLQIHSAKRPA
                     TWPLVAFSLFVPFLVLIFFVHR"
     gene            complement(6477..8837)
                     /locus_tag="VFQ79_12345"
     CDS             complement(6477..8837)
                     /locus_tag="VFQ79_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140502.1"
                     /translation="MISWGLRYLFLVPGMFAMAAAAVGELGEITGRITDPSSSVVQGA
                     IVVAECSGVRLETQTSSEGRYTLQPPAGTCRLLVRMDGFAPLSTDLKLSAGEHAVHDF
                     QLTMAGVADAITVTAGGFEQLVRDAPASVTIVRQEELQTRRVSDLAQALIDVEGVDVG
                     QNVGKTGGLTISVRGMPSDYTLMLIDGRRQNAPGSVTPNGFGETATSFMPPVAAIERI
                     EVVRGPMSTLYGSDAMGGVVNVITRRVGERWTGSVTLDGTLQTNTGYGNTGNGNFYFA
                     GPVISDRLGLAVRGSAFRRQAAALKYEDVGGNPVPITSFGLSPTRADVQNFGGRLSYV
                     INSNHDLYFDMDGMSQAYDNSDRQLGTVGIQGGYEEELKFRRWQAALSHTGRLGFGVI
                     DTSFTRNTTDTLGRTIPPGTPGRIAGDPRTLEATNTLLDSKLISEVGGKHILSVGGQW
                     WDADMIDGVAPDAFNFSQSAVFAEDEWRLLPKLALTGGVRYNYHTTFGSNTSPRAYLV
                     YNVSPLLTLKGGVSRGFKTPQLNQLATGIVGFGGQGTIPLIGSPGLKPETSTSTEAGA
                     ALTLKGVSLSVTAFNNQFKDKIAQGPGLENCSFRLSPDRPGCVDFGNWPNVDLFSQSI
                     NVDEAVTQGVETAMRLRLLRRLEVMNNYTYTQSEQRSGAQVGQPLVNTPKHMYNATTR
                     YLATSRLNLWLRIEARSNRTRGTAATAVAATDQIGPFKGYGMAHLGGGYQLTRRVTLN
                     AAINNLLNTDFLTYRPYVYNNATTYASVYNNLQEPRRVWMSLTYMF"
     gene            9355..10005
                     /locus_tag="VFQ79_12350"
     CDS             9355..10005
                     /locus_tag="VFQ79_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0140503.1"
                     /translation="MKSTTKAELAGAESASGDVDVVILDDDLDFRNYIEDLLKDEGKY
                     AVRSYAHPDDLFAACEERQPEIVLLDMKMGEFKGEHVLEQLLARYPGLCVIVVTGYPS
                     LENMRATFKMNVFDYLAKPFSLAQLRHVLENAINSFGLGRMAHDRLREKLGHRIKLLR
                     VERDWSLKDLAGATKLSISQISSIERGAHLPSIESLLAICRAFNKRASEVLASIDF"
     gene            complement(10006..10767)
                     /locus_tag="VFQ79_12355"
     CDS             complement(10006..10767)
                     /locus_tag="VFQ79_12355"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEU0140504.1"
                     /translation="MAFLPGKLGPVKASSYRLASRMVNALKAGYAVKSYEDLDECRLI
                     LVCVHDRNLARTVEELADSRINWLGKSVLLCDSWMGSDEFEKLVTQGAAAGSIWAVDT
                     SGPARLAVEGDQEALRELKRLVDYGPPKLLELKRCRKDLYWASTSFASSLLTPMLVAA
                     TRCLQKAGMHPRDASGLVERTVLKSLRGHMKSGVKSWSGPLAAGDAVQLERQIAAVAN
                     IEKKLGTYFQQSCVLAVEAFGRDAGWVRRLAPPNP"
     gene            complement(10824..>11714)
                     /locus_tag="VFQ79_12360"
     CDS             complement(10824..>11714)
                     /locus_tag="VFQ79_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU0140505.1"
                     /translation="LPSALDNRPLTFEEFENRVNQAVYVSATPGPYELTRSAGVVVEQ
                     IIRPTGLVDPEVQVRPIRGQVDDLLHEIRKRTESNERVLVTTLTKRMAEDLAEYYAEA
                     GVRCSYLHSEVSTLDRVKILRDLRRGEFDVLIGVNLLREGLDLPEVSLVAILDADKEG
                     FLRSSGSLIQTIGRAARHLNGTAILYADVMTDSMRHAISETNRRRRIQTDYNEQHGIT
                     PQSIVKPIDMSLVAIAEADYVTVPIEPSDEAQDLTPTQREEFLASLEQQMKEAAKRFE
                     FEKAAQLRDRIKALRAPQPV"
BASE COUNT         2311 a         3632 c         3462 g         2309 t
ORIGIN      
        1 cacagttttg ccacacatct ggtggaaggc ggagctgatc tgccaacagt ccaggcgatt
       61 ccgggccatg ccgacctgaa gcccacttcc atttatctcc atatgtcgga gcggcatctg
      121 agggcagcgg gcacaccgat cgataaggcc gaactgacaa acctcgatca agtgaagcgt
      181 tcgcgaaagc tgcacaagaa atgaatcggc cacccttcga ggtggccgat atcattcgcg
      241 cgcatggcaa cagatttatc gaaaagaacc gatcctggct gacgtggctt caactccgag
      301 tgctccctgt aatgccccga ttccgagatt ctatccgcaa tttaatttgc actgcaaacc
      361 tatttgtagt acattaccct gagaggcgac acccaacgtt ctgaatcgct acaacagagc
      421 gtgtcgctgt actggagaat gtgatggaca cctgccggct tgacgagctc gaacgtcgta
      481 cttatcgcac cgtgatcgat gacggactcg gagacatcgt cctgggtatt ttcttcgttg
      541 ctctcgggct tggcatcgct accgggttgg gctggctcgg agcgttggcc ggtgcggtcg
      601 cagttcctgc ctggaggggt ttgcgtaaga cctttgtcga ggctcggacc ggctacgtgc
      661 ggctgaaccg ctcccggagg gcacaactcc gtcgcggccc cttcatcgtc gccaccatcg
      721 cggtgctgat accgctcggg cttgtaggcg tcgaaggatt gctcggccgc gagttcggca
      781 agatccccgg gctagtcccg gtgctggtgg gagtcgggat tgggatccct gtagccgccg
      841 ccggctatct gttcgagata aggcgattat ccgtctacgc cgggctcatc tggctggctc
      901 tcgcggtagc ttacgtgggc cgcatcccgt acggctgggt gattttcgcc ttcggcgtcg
      961 tggtaatggg atcgggattg ttctacctcg ccagcttcat gcggcgttat ccggcgaagc
     1021 cgaacggagt cagcgccgat gactactgaa gcgatctcga gtcccggcac atctccgagc
     1081 cacatcgacc gcatagtgca cgagccaggg cgattgaaaa tcctcagcca actctacgtg
     1141 gtcgaatccg ccgattttct gtttatcatg caacacacag gattgactca gggaaatctg
     1201 tcctcgcata tcagcaagct ggaagcggcg ggctatgttg aggtgcagaa ggagttcgtc
     1261 aagaaacggc ccaggacgtt gctgcgtatg acccgcttgg gccgggaggc gttggaagtt
     1321 tacgtcaccg ggatgaagcg gctactagag gatgttccgg tcccgggccc ggcagccgtg
     1381 gaaaagtcca gaggcgcacg tcgcaggtct cgtgccagcc tcggaatttg tctggtggcc
     1441 ctgctggcta gcgtagcgaa tgcacggacg actgaggttc ggtttaactc ttacgacggc
     1501 tatccgatgc tcgggaagct tacgatgccg gagacgcgcg gacctcaccc ggtggtgatc
     1561 tatgtgcaaa ccgccgaagg gatgacagcg gacatgaaga ggcccggccg cgatgggacc
     1621 ttcagctact tcgatctcta tcgcacgaag ctggctgaat tgaacgttgg attcttcgcc
     1681 tatgagggac gcggcatccg cggcggcgac tcgcctccac gctatgaagc gatcgactgg
     1741 cctgtctata acaccagcac gctggaaaat aaagtccggg acgtgctgag cgcggtccag
     1801 gtagttcgga agcagcgagg cgtcaagctc tcccggatat tcctgatggg cgcgagcgag
     1861 ggcactctcc tggcggctga ggcggcatca cgcgccccgc ttcagatccg tggactggtt
     1921 ctctacgggg tgatggccac gacgatgagg gacgccttca aattcatggt cagcgacggc
     1981 ggctttctcg cataccgcgg tttcttcgat actgacaagg acggcatgat ttcaaagcag
     2041 gagttcgaga tcgatcccca acgctaccgc gaaagggcgc tccggaacgc ggacttcgag
     2101 aagttcgacc acaatcgaga tgggttgttt acagttgacg aaatgcggct gctatcaaaa
     2161 ccctacctcg acgccgtcga ttccgatgat tatcagatat tgaaccgatg ggcgagaacg
     2221 gctgctggag tgagcacacc agataattgg ttcaaggatc acttcgagca cgccccgatc
     2281 tggacattct tatcccgact ggacatgccg atcggacttt tccatggaga agcggacaca
     2341 caactgccgg tcggaccact gcggcagttg gaggcgagcg cgaagaaggc gagaaagtcc
     2401 aggatgatgt ttcactactt tgaaggtttg gaccacagcc tggacatcag gcagtacttt
     2461 gcgcgcggca tcttgccagc cggacacaag gctatttttg agtacatctc tcggcatgcg
     2521 cggtagcaag cgtcctgctg ccatcggcta gtaccgtaac gcggtaaacg taccagtaac
     2581 gatcacatca ttggtaccag ccgaattcct cccaaagtaa ccttccctga tcagaggcca
     2641 aacctcaaaa ttcggttgat gcaattggga ggacctattg aagcagatct ataagcgcct
     2701 agcggtgatg atggctcttg ttgcggcgcc ggcgctgccg gcctcgatca gcctggactt
     2761 cacaggcccg ctcgaggcac tccctggaca gagcttgagc ttgctgggag gctccatcgt
     2821 tgtaaccgcc acacgaggag gccaggaggc ggatatcacc tggagggaag gccgcggttt
     2881 gggagtagcc ggagggcaga gtgatgaaat cgactggagc gggaagagcg aaaagctgat
     2941 cttcagcctt accaatcccg gagcgagctt gtatctgacc ggtttcacct tgaactcgct
     3001 ttatgccgat gagtttttat ggttcgacga gaaaggtcag gtcaccatct cccgcaacgg
     3061 ttttgaggac cttacattca ccttcgccgg tatcgagcgc aaaggagacg gccatctgta
     3121 cgtgccgctg cccaccggcg tgcagtttga ccggctcacg ttcagcacgc agggcccgag
     3181 cctgttgagt gattttgcgg tcggcggtat cgatgtggcg actccagaac ccgcaaccta
     3241 cgcgctgatg ggtgccggat tgctggccct cggggccata cgccgccgca gttcctgacg
     3301 cggttacacc agcatggcgc cgaatgccgg actggccacc gcccgccaac ccgcgttctc
     3361 acgaacaagg aacagggcgg cgatctcgtg ccgggcacac atggcgaggc cgctgtccac
     3421 gcccagcacc atcagcgcgg tggaccatgc atcggcgtcc atgcagcggt catataccac
     3481 cgtcaccgac gatggcgggc tggtcaacgg cgcccgcgtt cgcggatcca gcgtgtgagc
     3541 atagcttctg ccttcgtgct cgaagcggaa gcgatagtcc cccgacgtgg cgacggcctt
     3601 cccgcacaac gccacttttg ctttgagcgg acaccccggc ggtggttcaa tccacaccca
     3661 ccacggctgt cctcccgctt tcactccccg cgcgcgtagt tctcctccga tctcgaccag
     3721 gtagctggaa gcgccgagtt gctccagcag cgcggcaagc aggtccacag cgaaaccttt
     3781 cgcgatcgag gagaaatcga ggcggacccc tccaggttgc agcgcacgcc cggccttggg
     3841 atcaagttcg atgtggcgcc agtcgtgaag gggcggcgcg agttcggcgg ctggcggtgg
     3901 cgacctgcga gggcccgacg gaccaaatcc ccactggtcg gcagcttcgc ccaaggacgg
     3961 atcgaatgcc ccttccgaga ggcgggcaat ttcgagcgcc gattccagca cacaccggaa
     4021 ctcgtccggc aacacctgcc agcggcccgc tggggcacgg ttatactcgc tcagcgccga
     4081 atcgggacgc cagtgactca tctggctcac aaccccctcg agcgtccatt gaatgcggcg
     4141 cagaaacaac tcgtcgggca gcgtctgggc cgcatagagg acagcagacc aggtggtccc
     4201 catcgtctca ccggaaaact cccggcgggt ctccgaggga cctggctcga ccagatccgc
     4261 cggtgacaag tgggcgggga tcgcgatcac tgtggcagaa cttcgagcgt cacaacgtaa
     4321 ttcgcacgat cgccggccgg catcggcatc tgccccggcc caccaggccc acccgggccc
     4381 ccaggtccgc caggtccacc cggccctccg gggccgcgcc ttgcggcgcc caccgtggcg
     4441 ttgagccagt acattcccgg ctgtgcaaag cggaccacga actttccgtc gctgccggtc
     4501 ttgaccgtct gttcgtgcag ttggtcccgg tagcggatgc cccccgggat caccatcacc
     4561 tccaggtccg ccacaggctt tccattgtga aggaactgga aggtagcctc ttcgcccgcc
     4621 accaggtcat taggatgcgt gatgggcacc agttccagat ccgcgcccga aggctggaga
     4681 gcctttgtgt ctggcttgcc cgcggtaacg aacagctcaa tccgattgtt catacgcgcg
     4741 atcttgagat cctgcgcttg ttgcggcacg gcggaagccg cctccgcggc ggttccccgc
     4801 cagatcttcg gctggccgtt ctcgctccac gaagcaaaga ccatattgtt cacgttagcg
     4861 atacgatagg tgccgggctg ctgcaaatgt acatcgaagg tgctgcggta tttgcccgtg
     4921 gcgacgttct gcggctgaat cgtcgagccg tcagggcagg taatcaccac gttgttgacc
     4981 cgcatcggaa cgtggtcgaa gaagaacagc tcattcgaga ccgccgcgtc caccgtgatc
     5041 caggtgtcgg tcttcgacaa cacggtcgcg gacggcagca tccactggcg gtgggccaga
     5101 agcgtggccg gcaatgcgat gacggccaaa gcggcaaaag cgggaaaaga tggaagtttc
     5161 atgagatctc ctatgtgcgc ggcggcgaac cgcggtgcaa ttacggcttg acagtgactt
     5221 gaaccccgcc gatctcgtgc gaaccctggg cctttgccgt ggcgggagct ttcgaggcag
     5281 gccattggaa gggaacccgg accagttccc ggccgccgac ttcccgggcg acctccacca
     5341 caacgtgata ggcgccgggc gccagcgacg caagctgcgg gcccgaatct ttgaaactca
     5401 gcttatgcac gccaggcggg cgtgtggccc ccgttacccc atcgatgggc agggtctggc
     5461 tgcgtccgga cttgcgccac caggtacgca agtccttcag ccaggtgtcg ccctgttctc
     5521 ccttcgcgga gctcttcagc atgtaccaga ctgcaagatc ggcggcaacg ctttgatcgg
     5581 cccgttcgat ccagatcgcg gcataaggcc ggtggtattc ggcgacctgg atctgtggga
     5641 tttcgacggt gatctcgagt tcggccccag tcagtacggg gctcaccagc aggccggtga
     5701 gcgcggcgga aggaagcaag gtcttcatgg gcaggatgtc tccttgatta acggtgtacg
     5761 aagaaaatca ataccaggaa cggtacgaac aggctgaagg ccaccaaggg ccacgtggcc
     5821 ggccgcttgg ccgagtggat ctgcaagagg ataagaccac taaagctgaa cacgagacag
     5881 gccacggcca aaacatcgat gtaaatgctc caggcgtgcc ccgtgtgccg gcctttgtgc
     5941 agatcgttga aataagcgat ccaaccgcgg tcggtctgct cgaaatgggc cagaccggta
     6001 ctgcggtcta ccgtcaccca ggcgtcgcct cccggacgcg gtagcgagac atagagctcg
     6061 tcgcggctcc attcgccggt tccatccccc gcggagccaa actcgcgagc cagccacgtt
     6121 gcaaccgctg ccggcagcgg cctccggcct tcctgctcgt ccggcgcggg cacgagcgcg
     6181 gcggcgaccg gcgccggggc ggcagcctca cgaacacgca cgaccggctc ggccgggatc
     6241 tggctcgcgt ggttcagcgt gataccggtg atcgtgaaaa gcagcatgcc gccgagcgtg
     6301 acacccgagc tgacccaatg ccagagcgtc acctggcgga ccagcgcggc gcggaaagac
     6361 cgcttgggaa cagagggccg cggcggcgct gccggatgat tgaccttgcc ggccggagtc
     6421 aacggcgcag tctcctgacg agccggtttg cggatggaag catacggcat tgcagttcag
     6481 aacatatacg tcaacgacat ccacactcgc cgcggttcct gaaggttgtt gtagacgctg
     6541 gcataggtgg tggcgttgtt gtagacatat ggccggtagg tgaggaagtc cgtgtttagc
     6601 aaattgttga ttgctgcgtt caacgtcacg cgcctggtga gttgatatcc tcctccgagg
     6661 tgcgccatac catagccttt aaaggggccg atctgatcgg tggcggcaac ggccgtggca
     6721 gcggtgccgc gcgtccggtt gctgcgggct tcaatccgca gccacaaatt aagacggctg
     6781 gtggcgagat agcgggtggt ggcattgtac atatgcttgg gggtgttcac cagtggctgt
     6841 cccacctgcg ccccactgcg ctgttcgctt tgcgtgtagg tatagttgtt catcacctcc
     6901 aggcggcgca acagccgcag gcgcatcgcc gtttccactc cctgggtcac cgcctcgtcc
     6961 acgttgatgc tttgcgagaa cagatctacg ttcggccaat ttccgaagtc gacgcagccc
     7021 gggcggtcgg gcgacaggcg gaagctgcaa ttctccaggc ccggcccctg ggcgatcttg
     7081 tccttgaact ggttgttgaa cgccgtgaca ctcaggctca cgcccttcag cgtcagcgcc
     7141 gcaccggcct cagtgctggt gcttgtctcc ggtttcagac cagggcttcc gatcagtgga
     7201 atggtcccct ggccgccaaa accgacgatg cccgtagcca actggttcaa ctgcggcgtc
     7261 ttgaagcccc ggctcacccc gcccttcagg gtcagcaacg gcgagacgtt gtacaccagg
     7321 tacgcccgcg gactcgtatt cgagccaaac gtcgtgtggt agttataacg gactccaccg
     7381 gtgagcgcca acttgggcag cagccgccac tcatcctcgg cgaacacggc gctctggctg
     7441 aagttgaatg catccggcgc cacgccgtcg atcatgtccg cgtcccacca ctggcccccc
     7501 accgaaagta tgtgtttccc gccgacctcg ctgatcagct tggaatccag cagcgtgttc
     7561 gtcgcctcta gtgtgcgtgg gtcgcccgca atccgtccag gagtgcccgg cgggatggtg
     7621 cgtccgagag tatcggtggt gttccgcgta aagcttgtgt cgatcacccc aaaacccagg
     7681 cgcccggtgt gggagagcgc ggcctgccag cggcggaact tcagctcctc ctcgtagccg
     7741 ccctggatgc ccaccgtgcc caactgccgg tcgctgttgt cataggcctg cgacatgccg
     7801 tccatgtcga agtacaggtc atggttggag ttgatgacat aggacagccg gccgccgaag
     7861 ttctgcacat cggcccgcgt cgggctaagc ccgaagctcg ttatcggcac cgggtttcct
     7921 ccgacgtcct catacttcaa cgccgcagcc tgccggcgga aggcgctgcc acgcacggcc
     7981 aatcccagcc ggtcggaaat caccggaccc gcgaaataaa agttgccgtt gcctgtattg
     8041 ccatagccgg tgttggtttg cagcgtgcca tccagggtca ccgatccggt ccagcgctcg
     8101 cctactctgc gagtgatcac gttcacgacg ccgcccatgg catcggaacc gtatagcgtc
     8161 gacatgggac cgcggaccac ctcgatgcgc tcaatcgcgg ccaccggcgg catgaagctt
     8221 gtcgccgttt ccccaaaccc gttcggcgtg acgctgccgg gcgcgttctg gcggcgtccg
     8281 tcgatcagca tcagcgtgta gtcgctcggc atgccccgga cgctgatggt cagccctccc
     8341 gtcttgccca cgttttgccc cacgtccaca ccctcgacat cgatcagcgc ctgcgccagg
     8401 tcggagactc tccgcgtttg aagttcctcc tgccgcacaa tcgtgacgct cgcgggcgcg
     8461 tcccgtacca actgttcaaa cccgcctgct gttaccgtaa tcgcgtcggc tacgccagcc
     8521 atggtgagct ggaaatcgtg gacggcgtgt tctcccgcgc ttagcttaag atctgtggac
     8581 aaaggcgcaa atccgtccat ccggacaagc aaccggcagg tcccggcagg cggctgtagc
     8641 gtatagcggc cttcggagct ggtctgggtc tccaggcgga cgccggagca ctccgccacg
     8701 acgatggctc cctgcaccac actcgatgag ggatcggtga tccggccggt aatttcgcct
     8761 aattcaccaa cggcagccgc cgccatcgcg aacatccccg ggactaaaaa caaataacgt
     8821 agaccccatg aaatcatctg tctttcctct tctcgaacgt gatctataat tgcaatcgac
     8881 ttgcgatatt aatttgcgga tggaactctc tgcgcttgtt tgccggcatc gccccaacct
     8941 ggtgatccgc taatatggag gtacccggtc cctaaggctg tcgatccggc aatggccagc
     9001 tccccctttg catccggtgc cgctctgcct caacgcacag cagttcgcgc atcggcctcc
     9061 tgcgtccagc cgcgcgtttc gaacgcaaaa cccagtctcg cgggagatct cgtccttgag
     9121 gcactcgaat agagaactcg ggtactcctg gatccggccg cactcaaagc acaccaaatg
     9181 cacatgatcc atagcaggtc ctgcttcgca aatgtgcatt tctcgcaagt cgcaatcctg
     9241 gaatgcctat taaatcctat ctgcaactgg gtgtcaacta aaaaacatcc ggaggatgac
     9301 ggaaactttt ccaccaacct gagttagaat gcagtgtatc cggcgagggg ctgcatgaaa
     9361 tccaccacca aggctgagct ggccggggcc gaaagcgctt ccggtgacgt cgatgtcgtc
     9421 attctcgatg acgatctcga ttttcgtaac tatatcgaag acctgttgaa agacgaaggg
     9481 aaatacgccg tccgtagtta cgcgcacccg gacgacctgt ttgccgcatg tgaagagcgg
     9541 cagccggaga tcgtcctgct cgacatgaag atgggcgagt tcaagggaga gcacgtgctg
     9601 gagcagctac tggcccggta tcccggcctg tgcgtgatcg tggtgaccgg ttatccctct
     9661 ctcgaaaata tgagagccac gttcaaaatg aatgtgttcg attatctcgc caagcccttt
     9721 tctctagccc aactccggca cgtattggaa aatgcgatca atagctttgg gctgggacga
     9781 atggcgcatg accgcctgag ggaaaagctg gggcatcgca tcaagctgct ccgcgtggag
     9841 cgggactggt cgttgaaaga ccttgccggc gcgaccaaac tgagtatttc gcagatcagt
     9901 tccattgagc gcggcgccca tctgccgagc atcgaaagct tgctggcgat ctgccgggcg
     9961 ttcaacaaaa gagcgtccga agtgctggcg tccatcgact tctgattacg ggttcggggg
    10021 tgctaagcgg cgcacccagc ctgcatcgcg gccgaatgct tccaccgcca gcacacagct
    10081 ctgctggaag taggttccaa gcttcttctc aatatttgcg accgctgcga tctgacgctc
    10141 cagttgtacg gcatcccccg cggccagcgg tccgctccag gattttactc cggatttcat
    10201 gtggccgcgc agagacttca gaaccgtcct ctcgaccagt cccgaggcgt ctcgcggatg
    10261 cataccggct ttctgcaagc atctcgtcgc cgccaccagc atcggcgtga gtagagagct
    10321 cgcaaaggaa gtactcgccc aatacaagtc tttccggcaa cgcttcagct ccagcagctt
    10381 cggtgggccg taatccacca gccgcttcaa ttcgcgcagc gcctcctggt caccctccac
    10441 ggccaggcgg gctggcccgg acgtatccac cgcccagatg gaaccagccg cggcgccctg
    10501 ggtcaccaac ttctcaaact catcgcttcc catccacgaa tcgcaaagca agacagattt
    10561 tccaagccaa ttgatacgcg agtcggccag ttcctccaca gtgcgggcca ggttccggtc
    10621 gtgcacacaa accagaataa ggcggcattc gtcgaggtct tcgtagcttt tcaccgcata
    10681 cccggccttc aaagcattca ccatacggct ggccagacgg taagacgatg ccttgacggg
    10741 tcccagcttc ccgggcagaa aggccatcac acaacatcgg gttaactggc ccgacccaac
    10801 caagccgata ttcagggagc gcttcataca ggctgaggcg cgcgaagcgc cttgatgcgg
    10861 tcgcgcaatt gcgccgcctt ctcgaattcg aaccgcttgg cggcctcttt catttgctgc
    10921 tccagggacg ctaaaaattc ctcgcgctga gtcggcgtca gatcctgcgc ctcgtcggac
    10981 ggctcgatgg gaaccgtgac atagtcggcc tccgcaatgg cgacaaggct catatcgatg
    11041 ggcttgacga tactctgcgg cgtgatgccg tgctgctcgt tatagtcggt ctgaatccgg
    11101 cggcggcggt tcgtctcgct gattgcatgc cgcatgctgt ccgtcatcac gtcggcatac
    11161 aggatagcgg ttccgttcag atgccgggcc gcacggccta tcgtttgaat cagcgatccg
    11221 gacgagcgta gaaacccttc cttatccgcg tcgagaatcg ccaccagcga gacctccgga
    11281 aggtccagcc cctcgcgaag caggttcacg ccgatcagaa cgtcgaattc gccgcgcctc
    11341 aggtctcgca gaatcttcac ccggtccagc gtgctcacct ccgagtggag atagctgcaa
    11401 cgcacgcccg cctccgcgta gtattccgcc aggtcttccg ccatccgctt ggtaagagtc
    11461 gtaaccagaa ctctttcgtt cgactcggtg cgcttgcgaa tttcatgaag cagatcgtct
    11521 acctgccccc gtatcggacg aacctgcact tccggatcca ccaacccggt cggccggata
    11581 atctgttcca ccaccacacc ggcggaacgc gtcaactcat acggtccagg cgtggcagag
    11641 acataaaccg cctggttgac cctgttctcg aattcttcaa acgtaagcgg ccggttgtcg
    11701 agggcgctgg gcag
//
LOCUS       DASTMK010000288         8335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_110158, whole genome shotgun sequence.
ACCESSION   DASTMK010000288 DASTMK010000000
VERSION     DASTMK010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110158"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFQ79_12365"
     CDS             complement(<1..831)
                     /locus_tag="VFQ79_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140506.1"
                     /translation="MDNQSVDRRSFIKQTAAGALTTSIFTGNVQGANDRISAAFIGVG
                     VMGSENLQAAMKQPGVVATAVCDVYQPNLERAGAIARRAGHQAKEVRDFRDILKDKSI
                     DVVCISTPDHWHPYMTVEACKAGKDVYVEKPACVAIDEGPKMIEAARKYGRIVQAGTW
                     QRSGEHFQKACEIVRSGELGEIAFTRTWIYNNTPREGIGNPPDAAPPNDLDWELWLGP
                     APKRQFNPNRFGVYPKKYSYFRYFWDYAGGQLTDSGIHVIDVVHMAFNERMPRSVVAA
                     G"
     gene            complement(861..2663)
                     /locus_tag="VFQ79_12370"
     CDS             complement(861..2663)
                     /locus_tag="VFQ79_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU0140507.1"
                     /translation="MQLHALDWFIVIASVLVCFVPALFFGRRSGKSTTEFFASGRSVP
                     WWLAGLSMVATTFASDTPNLVTNFVRTQGVAGNWQWWAFVLTGMATVFFYARLWRRSG
                     VLTDLEFYELRYSGRAASVVRGFRAVYLGLFFNCIIIATVNLAACKIAAILFGMSRWE
                     TLIFVGLLNVVFAAHSGLWGVLVIDMIQFFIKMSAVIAAAYFAVKLPQVGGLEGLVDK
                     VSNLRGPGGLNYLNVMPDFTNNWDLAVAIFIMPIAVQWWAVWYPGAEPGGGSFIAQRM
                     LASKSEKDSLGGTLFFNLAHYVLRPWPWILVGLASLIVYPTLDDIQHAFPDLDPTLIG
                     HDIAYPAMLRFLPVGWMGLMVGGLIAANSSTVLTHLNWGASYLVHDFYRRFMRRGASE
                     KHYVLAGRLGTIFIFLVSSALVFAMETAQDSFNLILQIGAGTGLLYLMRWFWWRITAW
                     CEVVAMVSSFGVSVLFLLLRRNGIALGTHRELLLTVIVTTICWLATAYLGPQTDRRTL
                     IDFYRKVRPFGPGWRQIRLEAGISDAEAAEHSRGENIPLALLGWVTGCVMIWSSLFTV
                     GNWLYGRTGYTIVLLVVFVASGLGLVSVIKRLWK"
     gene            complement(2669..3466)
                     /locus_tag="VFQ79_12375"
     CDS             complement(2669..3466)
                     /locus_tag="VFQ79_12375"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU0140508.1"
                     /translation="MSKIYYVGDWAIMLGPVFAETPFNYAWKGTDLFNYGQWLKNAIE
                     SDGRHEVTSVPTWDFYKLAPGRYEQILDEYDAFIFSDVEAKNFQLDPHFFDRTKFGSK
                     PLTFPDRVRLTVEALRGGKHMIFAGGWLSFNGESGKGGWGRTQLREILPVECLEFEDL
                     RESTEGWRAVAIQPEHRLFDGIDLTQMPPILGYNIVKPRAGCDVLAVWEGSTDPMLAA
                     GEFGKGRVLAYTSDPAPHWGCNFVYWDQYTQFWLNAVSWLTAESQDR"
     gene            complement(3482..5515)
                     /locus_tag="VFQ79_12380"
     CDS             complement(3482..5515)
                     /locus_tag="VFQ79_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU0140509.1"
                     /translation="MSKAGLILLVLMVVPVYANPALIPRPQRIETQEGQWELRRSSRI
                     SAASGAEEEAQKLASALSASTGFRIPVVSAGARPEDIAMVLEPGREDLGEEGYELSVS
                     QRGVRMVARRPAGLFYATQTFRQLLPAASWRRAPSAAGSFSAPYVKILDKPRFGWRGV
                     LLDPARHFIAKNMVLDMIDAMAMHKLNRLQLHLTDDQGWRVEIRAFPRLTARSAWRDG
                     TLIGHAREKPERYTSVPHGGYYTQEDIREIVAYAATRHIVVVPEVEMPGHARAWLSAY
                     PEFAVFPEKAAGMDLWRHWGVSKDVLAPRPKTLEACRRILDEICELFPSTWIHIGGDE
                     APRDQWRESEEMQALIRSLGLKNEDELQAWFTAEMSRYLASKGRRLVGWDEILAGATM
                     GGGEARTALAPNAIVMSWRGEKGGRTAAAAGHDVIMAPTQWTYLDYLQGPREEEPLGI
                     GGSVSLLRTYAYEPVPADLPPAAAARVLGSQAQLWSEYMPASESIGYMAFPRLCALAE
                     NFWSPGGARDIPGFLSRLEQHEQRLKAAGVRYRPLARRTSWPGEPGRVVAAARDAVIH
                     GTDIVRREDGALDQWKSEYTMVGWRVEIPSAAAYRVRLLLADPADANSIPLAAHIGGT
                     VLKPAARGTSDSIDFGIFKARTGGPHLLFLYPQGTPAAEGLPPVRGIEFVAER"
     gene            complement(5583..6014)
                     /gene="dut"
                     /locus_tag="VFQ79_12385"
     CDS             complement(5583..6014)
                     /gene="dut"
                     /locus_tag="VFQ79_12385"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU0140510.1"
                     /translation="MLVRIKRLRPDAILPRYAHGPMEDAGMDLHAVEDAVLDPGRPAA
                     VPTGIAIELPAGFEAQVRPRSGLALRHSITLPNSPATIDPGYRGEIRVIMLNLGTESY
                     HIRAGDRIAQMIVSRYEHVEWDESELNDSGRGERGFGSSGR"
     gene            6037..7476
                     /locus_tag="VFQ79_12390"
     CDS             6037..7476
                     /locus_tag="VFQ79_12390"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU0140511.1"
                     /translation="MPSEKPKSKVVAVAPMPPEKCAYALARYSRSPDSIHESVEWVRT
                     HDSQKFLESYYFQYGHASIADLGHVVLCFEGISELAATEIEDEQLWDGQAKSSRYQDF
                     SQAGFITPSDFDEDSAALYERTGESLLARYTQIHGRMCEFLSEKLPRPAAMKEDAYRR
                     NIAARAFDVARYVLFFGVPTNVGQVTSIRTLEKQIRRLKASCYSELREIANEMAAVCA
                     SAPACVWDRDAANEPVAPTLARHVNPDLHAAQSRADLRQWAQENLPAMAPEASKTVTL
                     IRPSDALADIAATLLYPVSNWPYSELYELVNGWSEARRREVVEVALRSQSRKDELLRG
                     FRSGPFVFDIVMDIGAYRDLHRHRRCQQFRQEYSPALGYETPEAIAEAGMTETYAAAF
                     EEAYNAFRLLPQTGREYLLPFGTLGRFLFKMDFAEAEYISRLRSGVKGHFSYRKIAWL
                     MREAMVEAEPELAAFMDATPPWIEDPLQR"
     gene            7701..>8335
                     /locus_tag="VFQ79_12395"
     CDS             7701..>8335
                     /locus_tag="VFQ79_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140512.1"
                     /translation="MRSLKAFTLASCLGLLTLLVPTANADTWDKKTILTVNEAISVPA
                     PHEKTGKVLEPGKYVMKLADSPSNRHIVQIFNEDENQLITTILAIPNYRLEPTGDTKF
                     TFWETPAGQPKALRAWFYPGDNFGQEFVYSPAQATEIAKVSGEPVPMTEADQESFSGA
                     DVASVDEAGTQRELDRETYQPPQETAQVQQPEPEVQEQPQVAQSQPVTQPR"
BASE COUNT         1766 a         2554 c         2404 g         1611 t
ORIGIN      
        1 gcccgcagcc accaccgacc gcggcatgcg ttcgttgaag gccatgtgca cgacatcaat
       61 gacgtggatt cccgaatcgg tcagttgccc cccggcatag tcccagaaat agcggaaata
      121 cgaatacttt ttcggataga caccgaagcg gttcgggtta aactgccttt tcggcgccgg
      181 acccagccag agttcccaat ccagatcgtt gggaggcgcc gcgtcaggcg ggttgccgat
      241 gccttcgcgc ggagtgttgt tgtagatcca ggtgcgcgtg aacgctattt cgccgagctc
      301 gccgcttcgc acaatctcac acgctttctg gaaatgctcg ccggagcgct gccacgtgcc
      361 ggcctgcacg atgcggccgt atttccgtgc agcctcgatc atcttcggcc cttcgtcaat
      421 cgcgacgcac gccggcttct ctacgtacac atccttcccg gccttgcacg cctcgacagt
      481 catataggga tgccagtggt caggcgtgga gatgcagaca acgtcgatgg atttgtcttt
      541 gaggatgtcg cggaagtcgc ggacctcttt ggcctggtgg cccgcccgcc gcgcgatcgc
      601 accggcccgc tccagattgg gctggtagac atcgcacacc gcggtcgcga ccactcccgg
      661 ctgcttcatg gcggcctgta ggttttcgct gcccatcact ccgacgccga tgaaggcggc
      721 ggaaatccgg tcgttagcac cctggacgtt tcccgtaaaa atcgatgtgg tcagggcgcc
      781 tgccgcagtc tgcttgatga aactccgccg gtcgacggac tggttatcca tgtttatgtc
      841 cttaaactat tatgcgcggt tcacttccat agccgcttga tcaccgatac cagccctaat
      901 ccactggcga cgaagacaac cagcagcacg atggtatatc ccgttcggcc atacagccag
      961 ttgcctactg tgaacaggga ggaccagatc ataacgcatc cggtaaccca tcccagaagc
     1021 gccagcggga tgttctctcc gcgggaatgt tccgcggctt cggcgtccga gatcccggcc
     1081 tccaggcgga tctgccgcca gcctggaccg aagggccgca ctttccggta aaaatcgatc
     1141 agcgtgcggc gatcggtctg cggtcccaaa taggcagtag ccaaccagca gatcgttgtc
     1201 acaatcacag tgagcagcaa ctcgcggtgt gttcccagcg cgatgccgtt cctgcgcagc
     1261 aggaggaaca gcaccgagac accaaacgag ctcaccattg cgacgacctc gcaccacgcg
     1321 gtaattctcc accaaaacca tcgcatcagg tacaacagcc cggtgcccgc gccgatctgc
     1381 aaaatcaggt tgaaactgtc ctgtgccgtc tccatggcga acacaagggc ggaagacacc
     1441 aggaaaatga atatcgttcc aagccgccct gccagcacgt agtgtttctc gcttgcgcca
     1501 cgccgcatga aacggcgata gaagtcgtgc accaggtacg atgcgcccca attcagatgc
     1561 gtcagcactg tcgacgaatt cgccgcgatg aggccgccaa ccatcaggcc catccagccg
     1621 acgggcagga acctgagcat ggccggatag gcgatgtcat gaccgatgag cgtcggatcc
     1681 agatccggaa aggcatgttg gatgtcgtcc aaggtgggat acacgatgag cgaagccaac
     1741 ccgaccagaa tccacggcca gggccgcaga acgtaatggg ccagattgaa gaacagggtg
     1801 ccgccgagag aatctttttc agatttcgac gccagcatcc gctgcgcgat gaagctgcct
     1861 ccgcccggct ccgcgccggg gtaccagacg gcccaccact gaaccgcgat gggcatgatg
     1921 aagatggcta ccgccaggtc ccagttgttc gtgaaatcgg gcatcacatt gagatagttc
     1981 agcccgcccg gaccacgcag gttcgagacc ttgtccacca gcccttctag gccgcctacc
     2041 tgcggcagct tcacggcgaa ataggcggcg gcaatgacag ccgacatttt gatgaagaac
     2101 tgaatcatgt caataaccag cacgccccac aggccggagt gcgcggcaaa caccacattc
     2161 agcaatccaa caaaaatcag cgtctcccac cggctcatac cgaaaaggat ggcggcaatt
     2221 ttgcaggcag ccaggttgac ggtggcgatg atgatgcagt taaaaaacag gccgagatac
     2281 accgcgcgaa aaccccgcac cacgctcgcc gcccggcccg agtagcgcag ctcataaaac
     2341 tcgaggtccg tcagcacgcc ggagcggcgc cacaagcggg cgtaaaagaa aaccgtcgcc
     2401 attcccgtca gcacgaaagc ccaccactgc cagttgcccg ccacgccctg ggttcgcaca
     2461 aagttggtaa ccaggttggg cgtgtcgctc gcaaaagtag tggcgaccat cgacagcccg
     2521 gcgagccacc atggaacgga ccggcccgac gcgaaaaatt ccgtcgtgct ctttccggac
     2581 cgcctgccga aaaacagcgc cggcacaaaa cacacgagta cggaagcgat gacaatgaac
     2641 caatcgagcg cgtgcagttg cataggttct aacgatcctg ggattcagcc gtcaaccaac
     2701 ttaccgcatt gagccaaaac tgcgtgtatt ggtcccaata gacaaagttg cagccccagt
     2761 gcggcgcggg gtcggacgtg taggccagca cacggccttt gccgaactcg ccggcggcca
     2821 gcataggatc cgtggagcct tcccaaacgg caagcacgtc gcaccccgcg cgcggtttaa
     2881 cgatgttgta gcccagtatc ggcggcattt gagttagatc gatgccatcg aacaggcggt
     2941 gttcgggttg tatggcaacg gctcgccacc cttctgtgct ttcccgcagg tcctcgaact
     3001 ccaggcattc caccggcaga atctcacgca actgagtccg gccccagccg ccctttcccg
     3061 actcaccgtt gaagctgagc cagccgcccg cgaagatcat atgcttgccg ccgcgtaatg
     3121 cttccacggt caggcgcaca cgatcgggaa acgtcagcgg cttgctgccg aatttcgtgc
     3181 gatcgaaaaa gtgcggatcg agctggaagt ttttcgcttc cacgtccgag aatatgaagg
     3241 catcgtattc gtccagaatt tgttcgtacc gccctggagc aagtttgtaa aaatcccacg
     3301 tagggacgct cgttacctca tgcctgccgt ccgattcgat agcgttcttc agccactgtc
     3361 cgtagttgaa taaatccgtg cctttccagg catagttgaa aggagtctca gcaaataccg
     3421 ggcccagcat gattgcccag tctccgacgt aatagatttt agacataagt gtggcggcgg
     3481 actaacgttc cgcgacgaat tcgattcctc tgaccggagg caggccctcc gcggccggtg
     3541 ttccctgcgg atacaggaac aacaggtgag gtccgccggt tctcgctttg aagatgccga
     3601 agtcgatact gtcgcttgtg ccgcgcgcgg cgggtttcaa cacggttcct ccaatgtgcg
     3661 ccgccagggg aatgctgttc gcatccgccg gatctgccag cagcagacga acgcggtatg
     3721 cggccgctga cgggatctcg acgcgccagc ctaccatcgt atactcactc ttccattgat
     3781 cgagcgctcc atcttcacgg cggacaatat cggttccgtg aatgaccgca tcacgtgccg
     3841 cagcgaccac gcgtcccggc tccccgggcc aactggtacg gcgtgccagc ggccggtatc
     3901 gcacgcccgc tgctttcaaa cgctgttcgt gctgttccag ccggctcagg aatccgggaa
     3961 tgtctctggc gccgcccgga ctccaaaagt tttccgccag cgcgcacagg cgagggaacg
     4021 ccatgtagcc gatggattcc gacgccggca tatattcgct ccatagttga gcctgggagc
     4081 caagcacgcg ggcggcggcg gccggtggta aatccgcggg caccggctcg taggcatacg
     4141 tgcgcagcag actcaccgaa cccccgatgc cgagcggttc ttcctctcgg ggtccctgca
     4201 ggtaatcgag gtaggtccat tgcgtggggg ccataatgac atcgtgcccg gccgcggcgg
     4261 cggtgcggcc tcctttttct ccacgccagg acatgacaat cgcgttcggc gccaatgccg
     4321 tccgggcttc cccgcccccc atggtggcgc cggcgagaat ttcatcccaa cccaccaacc
     4381 ggcggccctt gcttgccagg tagcggctca tctccgccgt gaaccacgct tgcagttcgt
     4441 cttcgttctt caagcccaga ctccggatca gcgcctgcat ctcctcgctc tcacgccact
     4501 gatcgcgcgg cgcttcgtct cccccgatat gaatccacgt ggaaggaaac agctcgcaga
     4561 tttcgtccag aatgcgccgg cacgcctcca gcgttttcgg gcggggtgcc agcacatctt
     4621 tgctgacgcc ccaatgcctc caaagatcca tccccgccgc tttttccgga aacaccgcaa
     4681 actccgggta agccgacaac caggcgcgcg cgtggccggg catttcgact tcgggcacga
     4741 caacgatgtg gcgcgtggcc gcgtaggcga cgatttcccg aatgtcttcc tgtgtgtaat
     4801 agccgccatg cggcacgctg gtgtagcgtt ccggtttctc ccgggcatgt ccaatcagag
     4861 tgccgtcgcg ccacgccgag cgcgccgtca aacgcggaaa cgcgcgaatc tccacgcgcc
     4921 agccttgatc gtcggtgagg tgcaactgaa ggcggttgag tttgtgcatc gccatcgcgt
     4981 cgatcatgtc cagcaccatg ttctttgcaa tgaagtggcg tgcggggtcc aacagaacgc
     5041 ccctccatcc gaaacgcggc ttgtccagga ttttcacata gggcgctgaa aacgacccgg
     5101 ccgcggaggg cgctctacgc cacgatgcgg ccggcagaag ctggcgaaaa gtttgtgtcg
     5161 cgtagaaaag gccggccgga cgtctggcta ccatccggac accacgctga ctcacactca
     5221 gctcgtatcc ttcctcaccc aaatcctcac ggccaggctc gagcaccatc gcgatatctt
     5281 ccggccgcgc gccggcggag acaacaggaa tgcgaaatcc cgtggaggcg ctcagtgcgg
     5341 atgcgagttt ttgggcctct tcttccgcgc cagaggcggc cgagatacgc gagcttcgcc
     5401 gcagttccca ctggccctcc tgcgtctcga tcctctgggg acggggaatc aacgcgggat
     5461 tcgcatatac aggcactacc atcagcacca ataaaatcag gccggccttc gacacggttc
     5521 acctctcgta taaagcttca gtgtagaccg gaaaaaggag ccctgttgtc gcgaattgta
     5581 ccttaccttc ccgaagaccc gaatccccgt tcgccgcggc ctgagtcatt gagctccgac
     5641 tcgtcccatt cgacgtgctc atagcgggag acgatcattt gggcaatgcg gtcgccggcg
     5701 cggatgtgat agctttctgt tccgaggttc agcataatga cgcggatttc gcccctgtag
     5761 ccgggatcga tggtggccgg gctgttgggc agagtgatgg agtggcgcag agcgaggccg
     5821 cttcgcgggc ggacctgtgc ctcaaaaccg gcgggcagct cgatggcgat tccggttggc
     5881 acggccgcgg gccgacccgg gtcgaggaca gcgtcttcca cggcgtgcag gtccattccg
     5941 gcgtcttcca tgggtccgtg cgcatagcgc ggcaatattg cgtccggccg gagccgtttg
     6001 attcgaacta gcatgaagta gctatcataa cgttcagtgc cttccgaaaa acccaagagc
     6061 aaagtagtgg cggtcgctcc gatgccgccc gagaagtgcg cctatgcact ggcgcgttac
     6121 agccgttcgc cggattccat tcatgagtcc gttgagtggg tgcgcactca cgattcgcaa
     6181 aaattcctgg agagctacta tttccagtac ggccatgcat ccatcgcgga tctggggcat
     6241 gtggttcttt gtttcgaggg catctccgaa ctggcggcga ccgagatcga ggacgaacaa
     6301 ctgtgggacg ggcaggcgaa atcgtcgagg taccaggatt tctcgcaagc cggattcata
     6361 accccttcgg acttcgacga ggactcggcg gccctgtatg aacgaaccgg ggaatcgctg
     6421 ctcgcccgct atacacagat ccacgggcgg atgtgcgagt ttctgagcga gaaactccct
     6481 cgccccgcgg caatgaagga ggacgcgtac agacgcaata ttgcggcgcg agctttcgac
     6541 gtcgcccgct acgtgctttt ttttggagta cccacgaacg ttgggcaggt gaccagcatt
     6601 cgtacgctcg aaaagcagat ccgccgcctg aaggcgtcct gctattcgga gttgcgggag
     6661 attgccaatg aaatggccgc ggtttgcgcc agtgctccgg cgtgtgtctg ggatcgcgac
     6721 gctgccaatg aaccggttgc accgacgctc gcacgccacg tgaacccgga cttgcatgcg
     6781 gcgcagtcgc gcgcagacct gcggcaatgg gcacaagaga atctgcccgc gatggcgccg
     6841 gaagcatcga aaaccgtgac cctcatccgc ccgtcggatg cgcttgccga cattgccgcc
     6901 acgctgcttt atcctgtcag caactggccc tattcggagt tgtacgagct ggtaaacggt
     6961 tggagtgaag cacgccggcg cgaagtggtg gaagtcgcgt tgcgttcgca gagcaggaaa
     7021 gacgaattgc ttcgcgggtt tcgaagcggt ccatttgtat tcgatatcgt gatggatatt
     7081 ggagcgtacc gcgacctgca tcgccacagg cgctgccagc agttccgcca ggaatattct
     7141 ccggcgcttg gatacgagac accggaagcg attgcggaag ccggtatgac agagacctat
     7201 gcggccgctt tcgaggaggc gtacaatgcc ttccgccttc tgccccagac cgggcgtgag
     7261 tacctgctgc cgttcggcac cctgggacgg ttcctgttca agatggattt tgccgaagcc
     7321 gaatatattt cgcgcttgcg aagcggtgtg aaaggtcatt tcagttaccg caaaatcgcg
     7381 tggctgatgc gggaagccat ggtggaagca gaaccggagc tggcagcgtt catggacgcg
     7441 acaccgccct ggatcgaaga cccgctgcaa cgctaatctg ttcagcctgt catcttttcc
     7501 gatgtaaaac ttgcagactg ccgtccgggt aaattctgcg atctcattac acttcaacaa
     7561 ttttgtgatt tggcaggtta cctgcctgta cacacggtag aggagggttc aatgctggga
     7621 ccccttgaaa gcgggacttc agaggcccgc aggctcgcga aaacgaaaaa gaaaagttga
     7681 aagttggagg agatgaaagc atgagaagct tgaaagcatt tacgttggcg tcttgcctgg
     7741 gcctgttgac cctgttagtg cctacggcta acgccgacac atgggacaag aagacaatct
     7801 tgacagtgaa cgaggcgata tcagttccgg cgcctcatga aaagaccgga aaagtcctgg
     7861 agcctggaaa atatgtcatg aagctcgcgg actctccgtc gaaccgtcac attgtccaga
     7921 tcttcaacga ggatgaaaat cagttgatta ccaccatcct cgctattccg aactaccggt
     7981 tggagccgac cggggacacg aaattcacgt tttgggaaac tcccgccggt cagccgaaag
     8041 cgttgcgggc atggttctat cctggtgaca actttggcca ggagtttgtc tattcacccg
     8101 cacaggctac cgagatagca aaagtttcgg gagagccggt tccaatgacc gaagccgacc
     8161 aggagtcgtt ctccggcgcc gatgtggcga gcgtcgatga ggccggtaca cagcgcgagt
     8221 tagatcgcga aacttaccag ccaccacagg agactgccca ggtgcagcag cctgagccgg
     8281 aagtgcagga acaaccccag gtcgctcaat ctcaaccagt aactcagcct cggga
//
LOCUS       DASTMK010000289         5187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_15277, whole genome shotgun sequence.
ACCESSION   DASTMK010000289 DASTMK010000000
VERSION     DASTMK010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15277"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFQ79_12400"
     CDS             complement(<1..650)
                     /locus_tag="VFQ79_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140513.1"
                     /translation="MQTSRRHFIQISAAAGLATRTAFSANDQIQFATIGIGGQGTGDT
                     HSALRTPGVKFVAASDVYDGRLTRAREVFGNDLFTTRDYREILNRKDVDAVIIGAPDH
                     WHMKMAVDAMNAGKDVYVEKPMVQLTGDGKPVVDAQKKTGRILQVGSQRVSSIVYQKA
                     QDLLKAGSIGELNMVEAWWDRNSAIGAWQYSIPPDATPETVDWDRFLGRAPKRPFEP"
     gene            complement(716..1081)
                     /gene="mnhG"
                     /locus_tag="VFQ79_12405"
     CDS             complement(716..1081)
                     /gene="mnhG"
                     /locus_tag="VFQ79_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEU0140514.1"
                     /translation="MMEWVTGFLMVLGATLVLLAAIGVVRMPDLFTRMQAATKAATLG
                     LGCVLAGAAIQLADLSTVVRAISIGAFIMLTSPVSTHVIARAAYLTRVPLWEGTIVDE
                     WREDMEAQTEDVTDSNTVN"
     gene            complement(1078..1344)
                     /locus_tag="VFQ79_12410"
     CDS             complement(1078..1344)
                     /locus_tag="VFQ79_12410"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0140515.1"
                     /translation="MPSDAVFAALVIVSTALLISFVRLVRGPTLPDRVVAVDLIGTSS
                     VALIVLGAAASGERAFLDAAMVIALVAFLGTIAYAGFARKENRE"
     gene            complement(1334..1831)
                     /locus_tag="VFQ79_12415"
     CDS             complement(1334..1831)
                     /locus_tag="VFQ79_12415"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEU0140516.1"
                     /translation="MILANLLLALAWAALNGRFTLDSLITGLVVGHLVLLVLAKGGVL
                     VMAEVSRVERALSLLAYLLWEIFLANVRVALDVVSIQHRMKPGVIRLPLDVTSDGEIL
                     LLAAMINITPGSVALGVSDDRKVMYVHVMHMRTPDDARRIIKEGFERRVMGLRGERKE
                     NQHAV"
     gene            complement(1828..3312)
                     /locus_tag="VFQ79_12420"
     CDS             complement(1828..3312)
                     /locus_tag="VFQ79_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0140517.1"
                     /translation="MNVLVLPIALPLFTAAVLLLAPSRPLLQRWISFAGACALAVTAV
                     LVFVRVEREGILVLQLGGWPAPFGITLVADVLSAMLVVATAVVGLAVALSAFSGVDPR
                     REGFGYHGLIHVLLMGVSGAFLTGDLFNLYVWFEVMLVASFVLMALHRTRAQLEAAFK
                     YVALNLIASSLFLTTLGLLYGGTGALNMAELATILPARENTAYETILAMLFLTAFGIK
                     AAMFPLFFWLPASYHTPPAAVGAIFAGLLTKVGVYSMIRVFTLLFPGDPAMLYTLLLA
                     QAGCTMIVGLVGALAQRDFRRILSFNLVGHLGYTTVGVALGTSAAMAGSIFYILHHIA
                     AITNLYLISGIFLRLRHTTSLSELGSISRERPLVAFLAAIPVFSLAGVPPLSGFIGKL
                     ALVRGAFGAGAWWTGAIILAVSLLTLLSMARLWSESFWKTAPHEDKSRLPGVILLPIA
                     FLSAITVAMTVAAGPLFQLAMKASDQLLNPALYIDAVLKGGPQP"
     gene            complement(3309..3656)
                     /locus_tag="VFQ79_12425"
     CDS             complement(3309..3656)
                     /locus_tag="VFQ79_12425"
                     /inference="COORDINATES: protein motif:HMM:NF006573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEU0140518.1"
                     /translation="MELILAIASGILFGTGFYLMLRRRLAQLIIGLGLLSNGANLLIF
                     TAGGLTRARAPVIAEDASQLVRPFADPVPQALVLTAIVIGFGVLAFSLVLAHGIHTMA
                     GTDDIDEIRSGGG"
     gene            complement(3660..4061)
                     /locus_tag="VFQ79_12430"
     CDS             complement(3660..4061)
                     /locus_tag="VFQ79_12430"
                     /inference="COORDINATES: protein motif:HMM:NF009163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit B"
                     /protein_id="HEU0140519.1"
                     /translation="MTTTSLILRTGARVLMPLLLLFGVFLLLRGHNAPGGGFAGGLVV
                     AAAYILHFFAFGLDAARRALLTRPERLIGSGLLLAVGSGILPVFFGRPFLTSLWLDPA
                     SGIGTPMLFDLGVFLVVIGVVLIMAFTLAEE"
     gene            complement(4058..>5187)
                     /gene="mbhE"
                     /locus_tag="VFQ79_12435"
     CDS             complement(4058..>5187)
                     /gene="mbhE"
                     /locus_tag="VFQ79_12435"
                     /inference="COORDINATES: protein motif:HMM:NF042818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HEU0140520.1"
                     /translation="FLGFLGKELLYESLLAWSTTLLIGAIAASALLGTAGLIAGVSPF
                     AGALRGRAPEHRVPAGLTVPALILAFLGLIAGIVPGLVELPAGLASGAILRRPVDASL
                     SMWHGFTPVLGLSLATLALVALLYAYRDLLRRRIWPRSLGSERLYTSTVRGLDALSAW
                     ASPMLQSASLRSYLLVLVTTAGILIFATLVFEGLPGWPAVMDVQPHEACAAILIVAGA
                     ISAVWAKSSITAVLSLGVSGYGVALTYLFFGAPDLAMTQFSVETLTAVIFVLVFYHFR
                     DFGSLSPWTDRLRGAAVSVLFGGSIAVVLLFVGANITPFRLANYFAEMGLPLAHGRNI
                     VNVILVDFRALDTMGEITVLATAAFGVRALMRISRKERKNG"
BASE COUNT         1193 a         1655 c         1522 g          817 t
ORIGIN      
        1 ggctcgaagg gacgtttggg cgccctgccg agaaaacggt cccagtccac ggtttccggt
       61 gtcgcgtcgg gcggaatgga gtattgccag gcgccgatgg cggagttgcg gtcccaccac
      121 gcctccacca tgttcagctc accgatggaa ccggccttca gaaggtcctg tgccttctga
      181 taaacaatgg agctcacgcg ctggctccct acctggagaa tgcggccggt cttcttctgc
      241 gcgtctacca ccggcttccc atcgccggta agctgcacca tcggcttttc gacgtaaaca
      301 tctttaccgg cattcatcgc gtccaccgcc atcttcatgt gccagtggtc cggggcgccg
      361 ataataaccg cgtctacgtc tttacggttc aggatttcgc gatagtcgcg cgtggtaaac
      421 aggtcgttcc cgaaaacttc cctggcgcgc gtgagccgcc cgtcgtacac gtcgctggct
      481 gccacgaact tcacgcctgg agtacgaagc gctgagtgag tgtcgccggt accctgtcct
      541 cctatgccga tggttgcgaa ctgaatctga tcgttagcgg aaaatgccgt tctggttgcc
      601 aggcccgcgg ctgctgaaat ttgtatgaaa tgacgtctac tagtctgcat tgaaggaatt
      661 actcctgttg agggcgttgg aggggcggcg tcgatatcgc atctgccgga tgttttcagt
      721 ttaccgtgtt cgaatctgta acatcctccg tctgcgcctc catgtcctca cgccattcat
      781 ccacgattgt tccctcccac aagggcacgc gcgtgagata cgccgcacgg gcgatcacgt
      841 gagtggaaac cggcgacgtc aacataataa acgctccaat gctgatggcc cgaaccactg
      901 tggataaatc ggcgagttga atcgccgctc ccgccagtac gcagccgagc cccagagtcg
      961 ctgctttcgt tgccgcctgc atccgcgtaa acaggtccgg catccgcact acgccgatgg
     1021 cggccagcaa caccagcgtg gctccaagca ccatcagaaa tcccgtcacc cactccatca
     1081 ttcccgattc tccttcctgg caaacccggc gtacgcgatc gtccccagaa aggcgacgag
     1141 cgcaataacc attgcagcat ccaggaacgc acgctctccg ctagcggcgg ctcccagcac
     1201 aatgagcgcg accgaggagg tacctatcag gtccaccgcg acaacgcggt ccggtagcgt
     1261 cggcccgcgc accagccgga cgaatgagat aagcagcgcg gtcgagacga tcaccagcgc
     1321 cgcgaatacg gcatcagacg gcatgttggt tctccttgcg ttcccctcgc agtcccatca
     1381 cacggcgctc aaatccttcc ttgatgatgc gccgtgcgtc gtcaggagtc cgcatatgca
     1441 tgacgtgcac gtacatcact ttgcggtcgt cggaaacgcc cagcgccacg ctgccggggg
     1501 tgatattgat catcgcggcc agcagcagaa tctcgccgtc gctggtgaca tcgagcggca
     1561 gccggatgac ccccggcttc atccggtgtt ggatgctgac gacgtcgagc gcgacacgga
     1621 cgttggcgag gaagatctcc caaagcagat aggccagcag cgacaatgcc cgttccacgc
     1681 ggcttacttc ggccatcacc aacacgccgc cctttgccag aaccagcagc accaggtgtc
     1741 caaccaccag gcccgtaatc aggctgtcga gggtgaagcg gccattcagc gccgcccatg
     1801 ccagtgccag aagcagattc gcgagaatca cggctggggt cctcctttca acaccgcatc
     1861 gatatagagc gccggattga gcaactggtc agacgccttc attgcgagct ggaatagcgg
     1921 tccggcggca accgtcattg ccaccgtaat agcgctgaga aacgcgatag gaagcagtat
     1981 cactccaggc agccgtgact tgtcttcatg cggcgcggtc ttccagaagg attcggacca
     2041 cagccgtgcc atggataaca gtgtcagtaa gctcacggcc aggatgatgg cgccggtcca
     2101 ccatgcgccg gcgccgaagg cgcctcgcac cagcgcaagc ttgccgatga acccggaaag
     2161 gggcggcact cccgccagcg aaaacaccgg gatggcggcc aggaaggcga ccagcgggcg
     2221 ttcgcgagat atcgaaccca gctccgagag agacgttgtg tgccgaagcc gcagaaaaat
     2281 gccgctgatg aggtaaaggt tggtaatcgc ggcgatgtgg tgaaggatat agaagatgga
     2341 tccggccatc gcggcggaag tacccagggc cactcccacg gtcgtgtagc cgagatgtcc
     2401 taccaggttg aacgacagga tacgcctgaa atcccgttgc gcaagcgccc ccaccagtcc
     2461 cacaatcatc gtgcaccccg cctgcgccag cagcagagta taaagcattg cgggatcgcc
     2521 cgggaacaat aacgtgaata cccgaatcat cgagtacacg ccgacttttg tcagcagccc
     2581 ggcgaagatc gcgcccacgg ccgccggcgg cgtgtggtac gaagcgggaa gccagaaaaa
     2641 cagcgggaac atcgcggctt tgatcccgaa tgccgtcaga aacagcatcg ccagaatggt
     2701 ttcgtacgcc gtgttttccc gcgctggcag aatggtggcc agttccgcca tgttcagcgc
     2761 gcctgtaccg ccataaagca gcccgagcgt ggtcaggaat aacgaggaag cgatcaggtt
     2821 caaagcgaca tacttgaaag ccgcttccaa ctgcgcgcgt gtccggtgga gcgccatcag
     2881 aacgaaggag gccaccagca tcacttcgaa ccagacatac aggttgaaaa gatcgccggt
     2941 gagaaatgcg cccgacacgc ccatgagaag cacgtgtatc agcccgtgat agccgaaacc
     3001 ttcgcgcctg gggtcgacgc cggaaaaagc cgacaaggcc acggcgagtc ccacaaccgc
     3061 cgtcgccact accagcatgg ctgacagaac gtccgccacc agggtgatgc cgaacggcgc
     3121 cggccatcca cccaattgca gcacgaggat tccctcgcgc tccacgcgca cgaacaccag
     3181 aacggccgtg acggctaatg cgcaggcgcc ggcaaaggag atccatcgtt gaagcagagg
     3241 gcggctgggc gcgagcagca gcactgccgc ggtaaacagc ggcagcgcga tgggaagcac
     3301 cagaacattc atccgccgcc gctcctgatc tcgtcgatgt cgtccgtgcc ggccatggtg
     3361 tgaatgccgt gtgccagcac cagcgaaaac gcgagcacgc cgaagccgat cacgatagca
     3421 gtgaggacca gcgcttgcgg caccgggtcg gcgaacgggc ggaccagttg agaggcatcc
     3481 tcggcgatca ccggcgcacg ggcgcgagtc agacctcccg cggtgaagat cagcaggttc
     3541 gctccattgc tcaacaggcc caggccaatg atcagttgcg ccagccgccg ccgcagcatc
     3601 aggtaaaagc ctgtgccgaa cagaatgccg gaggcaatgg ccagtatcaa ttccaagttc
     3661 tattcctccg cgagcgtaaa agccatgatc aacacgacgc caatcaccac caggaatacg
     3721 cccaggtcga acagcatggg agtgccgatt ccacttgccg gatcgagcca gagcgaggta
     3781 agaaagggcc ggccgaaaaa caccggaaga attccgctcc ccaccgcgag cagcagacct
     3841 gagccgatca gccgctccgg ccgggtcaac agggcacgcc gcgccgcatc caatccgaac
     3901 gcaaaaaaat gcaggatata cgcggccgcc accaccaatc cgccggcaaa gcctcccccc
     3961 ggcgcattgt ggccgcgcaa tagcaggaag acgccgaaca gcaggaggag cggcatcagg
     4021 acgcgcgcgc cagtgcgcag gatcaacgat gtcgtcgtca tccgttcttt ctctccttcc
     4081 ggctgatgcg catgagcgcg cgtactccga aggcggcggt ggccagcacg gtaatttcac
     4141 ccatcgtgtc cagcgcgcgg aaatcaacca gaatcacgtt tacgatattg cggccgtggg
     4201 ccagcggcag gcccatttca gcgaaatagt tggccagccg gaaaggagtg atattagcgc
     4261 cgacaaacaa cagcaccacc gcgatcgatc ctccgaacaa caccgatacc gccgcgccgc
     4321 gcagccggtc ggtccagggg gacagcgacc cgaaatcccg gaaatgataa aacaccagga
     4381 cgaagatcac cgccgtcagg gtctccacgg agaactgtgt catcgccaga tccggagcgc
     4441 caaagaaaag atacgtgagc gccacaccat agccggaaac acctagcgat agcacggctg
     4501 tgatgctcga tttcgcccag acagccgaaa tggcgccagc cacaatcaga atggccgcgc
     4561 aggcttcatg cggttggaca tccattaccg cgggccagcc cggaagcccc tcaaacacga
     4621 gcgttgcgaa aatcagtata cccgcggtgg tgacgagcac aagcagatac gaccgtagcg
     4681 aagcgctctg aagcatcggc gaggcccacg cgctgagagc gtcgaggccg cgcacagtcg
     4741 atgtatagag ccgttccgac ccaagcgatc gcggccagat gcggcgccgc agcaaatcgc
     4801 gataggcata gagcagagcg accagcgcca gcgtggccag actgaggccc agaaccggcg
     4861 tgaagccgtg ccacatcgaa aggctcgcat ccaccgggcg gcgcaggatg gcgccggacg
     4921 caagcccggc cgggagttcc accaaaccgg ggacgattcc cgcaatgaga cccaagaaag
     4981 ccagaatcag cgcgggtacg gtgagcccgg caggaacgcg atgctccggc gcccttcctc
     5041 gcagagctcc cgcaaaagga gagacgccgg cgatcagacc ggccgtgccg agcagtgcac
     5101 tcgcggcgat tgccccaatc agcagcgtgg tcgaccatgc cagcaaggat tcatagagca
     5161 gttccttgcc gaggaagccc agaaaga
//
LOCUS       DASTMK010000290         4957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_302114, whole genome shotgun sequence.
ACCESSION   DASTMK010000290 DASTMK010000000
VERSION     DASTMK010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302114"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1398
                     /locus_tag="VFQ79_12440"
     CDS             214..1398
                     /locus_tag="VFQ79_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0140521.1"
                     /translation="MITMSLATDTTQPVRGNTTISDLKITNVEAIYLRLPHVRELCDS
                     GQDALIVKVSTNAGITGIGEVDSAPLAVKGAIEGPFSHTTASGLREIVIGEDPFQTEY
                     LWQKMYRQSIYGGRSGVGFHAISGIDMALWDIKGKALGLPIWKLLGGGFHKKIRPYAS
                     TLFGATPEETGERGRRFRDRGFTAVKFGWEPMGQDEKTDIALVREARKGLGDDADLMI
                     DAGLVWDAKTAIQRARAFSEYNIFWLEEPLLPDDYEGYRKLSEATDVRIAAGEEESGR
                     RSFIQLMDQGRVDLIQVDLTRCGGFTEAMKIASLAADRGVPVVNHGFTTYVNVAAALH
                     FLNSIPRSFILEFVVEEETSLREHITRQKIVSVDGYVEMPSAPGLGIDLNEEALEKFR
                     VA"
     gene            1511..4786
                     /locus_tag="VFQ79_12445"
     CDS             1511..4786
                     /locus_tag="VFQ79_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140522.1"
                     /translation="MKRLFRIVTLLVCALTLSMNPGSLQAQVLYGSIVGVVQDATGSV
                     IPGATVLAVNPETRQERTVATDGSGRYSLPNLAAGNYEITVTASGFRTLTRRSVSVSI
                     NTIAREDFRLEIGALTEQVTVTASAARLQTETGAVQSDLMAREVTDLPLAKYRNYQTL
                     INLIPGATPGRFQNVNNPMKALTTNVNGVNRNNNATKLDGSPNRFTWLPHHTLYVPPA
                     ETIEAVSISTNSFDAEQGMAGGAAITIATKSGTNDFHGSAFSLHENSKWGAKNFFFRG
                     GPKTPKSLINIFGGTLGGPIKRDKLFFFGGWERVRERVNYGGLFTVPTLDQRNGDFSA
                     YPATIYDPGTGNADGSGRTPFPNRIIPSNRLSPVATRFQEWIPLPNLSGVNANYFNSA
                     TQVLDRDNFDGKVNWNRTSSHAVFAKYSAMRALHVGEFALGQAGGDCLCAGGVGRGRT
                     RVQLASVGHTLTFSPTLLWDGTLSWNRIGSTHESQDFGTNFGSEVLEIPGTNGPDRRQ
                     SGLPDFLVSGYSTLGNRNPWWPYFWNDMTYSTTHNFSWKKGAHEIRWGYEGLRLNLNH
                     WQPELGGGPRGSFDFNQEATGLRGSPTTQFNGWASFLLGLPQQARKSVQWIKMTGYEW
                     QHGLYVRDRWEVTRHLTLSLGVRYELYPLMTRANYGGIEQWDETTNVVRIGGLGGNPK
                     DLGISTSKKLFAPRIGLAYRAADNLVIRTGYGITYNPMPLARPLRSVYPLTIAQDFTG
                     INAYQPFRSIEEGIPDFGGPELSSGSVPLPGTVAMITIAGDSINRGYVQSWNFFVEGK
                     LPGDFVTSVGYVGTQTVNSFGQMEANVAAPGRGNLGRRFVPIYNRTAATSYWNGQFSA
                     NYHALQVAINRRAAAGLTLKAAYTYSKAINFTDDDGGVGVMFNWEPAFYRNRALAGYD
                     IPHMLQLGYVYELPFGKGKNMANTGVASVLLGGWQANGIFSAVQGRPFTVAASAGSLD
                     APGNSQTADQVKPEVEKIGSLAQFYDRTSFAAPTGAARFGTTGRNLLRGPGIVNLDLS
                     LFRNFNLTERYILQFRAEAANLSNTPHFGNPSNNVNSGNFMQITSAENDQRTIRFGLR
                     LQF"
BASE COUNT         1077 a         1453 c         1482 g          945 t
ORIGIN      
        1 ggtgatcccg gtgacaagaa acatcaggac gaggacccgg tagcgggtga agctggatat
       61 ctcaccgata tattttttag aaagttggag ttcggccatc cgcccggtct ctgaagctac
      121 aagagccgag tataggacag aagaactgag attgaaagtg ttaccggcat tcagacacca
      181 cttgacatat aaatatattt ttggaatatt tttatgataa caatgagcct ggcaaccgat
      241 acaacacaac ctgttcgcgg aaacaccact ataagcgacc tgaagatcac caacgtggaa
      301 gcaatctatc tcaggttgcc gcacgtccgt gaactttgcg acagcgggca ggacgcactc
      361 atcgtcaaag tctccacgaa cgcgggcata acggggatcg gggaagtgga ctccgcaccc
      421 ctggctgtga agggagccat agagggcccg ttttcccaca ccaccgccag cggattacgg
      481 gaaatcgtaa tcggggaaga cccctttcag accgaatacc tgtggcagaa gatgtaccgc
      541 cagagcatct acggcgggcg cagcggcgtc ggcttccacg ccatcagcgg tatcgacatg
      601 gccttgtggg atatcaaggg caaggccctc ggactcccca tctggaaact gctgggcggc
      661 ggcttccaca aaaaaatccg cccgtatgcg agcacgcttt tcggcgccac tcccgaggaa
      721 accggcgaac ggggacgccg tttccgcgac cgtggtttta cagccgtaaa attcggctgg
      781 gaacccatgg gccaggacga gaagaccgac attgcacttg tgcgtgaggc gcgtaaaggg
      841 ttgggcgacg acgccgacct gatgatcgac gccggtctgg tctgggatgc caagactgcc
      901 atccagcgcg cacgtgcctt ctccgaatac aacatcttct ggctcgagga gccgctgctg
      961 ccagacgatt acgagggcta ccggaagttg tcggaagcca cggatgtccg tatcgccgcc
     1021 ggcgaagagg aaagcggtcg gcggtcgttt atccagttga tggaccaggg gcgtgtggat
     1081 ctgattcaag tcgacctcac acgatgcggc ggcttcacgg aagcgatgaa gatcgcaagc
     1141 ctcgcggcag accgcggcgt tccggttgta aaccacggct tcaccacgta cgtgaacgtg
     1201 gcggccgcac tccatttcct gaactcgata ccccgttcgt tcattctgga gttcgtggtc
     1261 gaggaggaaa catcgctgcg ggaacacata accaggcaaa agattgtttc tgtggatgga
     1321 tacgtcgaaa tgccctcggc gccgggtctg ggcatcgatc tcaatgaaga ggcgttggag
     1381 aaatttcgcg tagcttgatc ggatgagaag gcgagaggcg gatgctttgg cgtctcttgc
     1441 gcggctgtgg tgggaatcgc cggcacggat cgagggccgt tgccgctgga aaggcttgtt
     1501 gaggggcaac atgaaaagac tctttcggat agtaacgcta ttggtttgcg cattgactct
     1561 gtctatgaat ccagggtctc tgcaggcgca agttctgtac ggttccatcg tcggtgtggt
     1621 gcaggatgcc acgggctccg ttattcccgg cgcaacggtg ctcgccgtga atccggaaac
     1681 gcgtcaggaa agaacggtcg caaccgacgg gtccgggcgg tactccctcc ccaacctggc
     1741 ggcgggcaac tacgaaatca ccgtcaccgc ttcggggttc cggacgttaa ccaggagaag
     1801 tgtttccgtg tccatcaaca cgatcgcgcg cgaagatttc cggttggaaa ttggcgcgct
     1861 gacagagcag gtgactgtaa ccgccagcgc ggcacggctg caaacggaga ccggtgcggt
     1921 acagagcgat ttgatggcga gggaagtgac agacctcccc ctcgcaaaat accgtaatta
     1981 ccagacgctc atcaatctca tcccgggcgc cacccccggg cgttttcaga acgtcaacaa
     2041 tcccatgaag gcgctcacga cgaatgtgaa cggcgtgaac cgcaataaca acgcgacgaa
     2101 gctcgatggt tcgccgaacc ggttcacgtg gcttccgcat cacacgcttt atgtgcctcc
     2161 ggcagagacg atcgaggcgg taagcatctc cactaacagc ttcgacgccg aacaggggat
     2221 ggcgggcggc gccgcgatca cgatcgcaac caagtcgggc acgaatgatt ttcacggctc
     2281 ggcgttctcg cttcatgaga acagcaagtg gggcgcgaag aatttctttt tccgcggcgg
     2341 cccgaagacc ccgaaaagcc tgatcaacat tttcggcggt acgcttggcg gaccgatcaa
     2401 acgcgacaag cttttcttct tcggcggctg ggaacgcgtg cgggagcggg taaactacgg
     2461 cgggctcttc acagtcccca ccctcgacca gcggaacggt gatttcagcg cctaccccgc
     2521 gacgatctac gaccccggaa cgggcaacgc ggatggttcg ggaaggactc ccttccccaa
     2581 ccgcatcatt ccttccaatc gcctgagtcc cgtggcgacc aggtttcagg agtggattcc
     2641 gctgcctaac ctgtccggtg tgaatgcgaa ctacttcaat tccgcaacgc aggtgctgga
     2701 ccgcgacaac ttcgacggta aggtcaactg gaaccgcacc agcagccacg ccgtgtttgc
     2761 gaagtacagc gccatgcgcg cgcttcatgt gggcgagttc gcgctcggcc aggctggtgg
     2821 agactgtctc tgcgccggcg gtgtgggcag aggccggaca cgcgtacaac tcgcctctgt
     2881 cgggcacact ttgaccttct cgccaactct gttgtgggac ggcaccctat cctggaatcg
     2941 catcggctca acgcatgagt ctcaggactt cggcacgaat ttcggcagtg aggtgctcga
     3001 aatccccggc acgaacgggc cggaccggcg gcagagcggt ttgccggatt tcctggtcag
     3061 cggctactcc acgctcggca accggaatcc ctggtggcct tacttttgga atgacatgac
     3121 gtactcgaca acgcacaact tcagttggaa gaagggagcg cacgaaatcc gctggggcta
     3181 cgaagggctt cgtctcaatc tgaatcactg gcagccggaa ttgggcggcg gtccacgcgg
     3241 ctctttcgac ttcaaccagg aggcgacggg gctgcgcgga agtcccacaa cgcaattcaa
     3301 cggctgggct tcgtttctgc tcggactgcc gcagcaggcc cgcaagagcg tccagtggat
     3361 caagatgacg ggttacgaat ggcagcacgg tttgtacgtc cgcgaccgct gggaagttac
     3421 gcggcacctg acgctgagcc tcggagtgcg ctacgaattg tatcctttga tgacgcgcgc
     3481 aaactatggc ggcatcgagc agtgggatga aaccacgaac gttgtccgta taggcggcct
     3541 cggcggcaac ccgaaggacc ttggcatctc gaccagcaag aagttgtttg cgcctcgaat
     3601 tggccttgcc taccgggccg ccgacaacct ggtcatccga accggctacg gtattacgta
     3661 caaccccatg ccactggcgc ggccgctgcg tagcgtttac ccgctcacga tcgcgcagga
     3721 tttcaccggt atcaacgcct atcagccgtt ccgttccatc gaggaaggaa ttccggattt
     3781 cggcggcccg gagctttcca gcggttcggt cccactgccg ggcaccgtgg cgatgattac
     3841 gatcgcgggg gacagcatta accgcggcta cgtacagtcc tggaatttct tcgtcgaagg
     3901 caaattgccc ggcgacttcg taacttcggt gggctacgtc ggcacgcaga cggtcaacag
     3961 cttcgggcag atggaggcaa acgtcgccgc gcccggcaga ggtaatcttg gccgccggtt
     4021 cgttccgata tacaaccgga ctgccgccac ttcctattgg aacggccagt tcagcgccaa
     4081 ctatcacgcg ttgcaggtgg ccatcaaccg gcgcgccgct gcggggctga ctctaaaagc
     4141 ggcgtacacg tattcaaaag ccatcaactt caccgacgat gatggcggcg taggcgtcat
     4201 gttcaactgg gagccggcat tttaccggaa ccgcgcttta gccggatacg acatcccgca
     4261 catgctgcaa ctcggctacg tatacgaact tccattcggc aagggcaaga acatggcaaa
     4321 caccggagtg gcgtcggtgc ttctcggcgg atggcaggcg aacggaatct tcagcgctgt
     4381 gcagggcaga cccttcacgg tggcggcttc cgcaggctcg ttggacgcgc ctggaaacag
     4441 ccaaacggcg gaccaggtga agccggaggt tgagaagatc gggagccttg ctcagtttta
     4501 cgataggacc agcttcgcgg ctcccaccgg cgcagcgcgt ttcggaacta cgggtaggaa
     4561 ccttctccgc ggccctggca tcgtaaacct tgatctcagc ctgttccgga acttcaacct
     4621 gacggaacgc tacatccttc agtttcgcgc cgaggctgcc aacctgagca acacgccaca
     4681 tttcggcaac ccgtcgaata acgtgaacag cggcaacttc atgcaaatta ccagcgccga
     4741 gaacgaccag cgcaccatcc gcttcggcct gcgcctccaa ttctgaaaac cgtaggtgaa
     4801 gagcagcggc aactttcgca taggcattac gccggacttc gagaccgagg caaacggagt
     4861 actctctccc gctttagcgg aggtgctgga gcccgccgcc ggcgttcggt acgagatcat
     4921 gccggacacc ggcggcgtcg gcgacccggc ggtgctc
//
LOCUS       DASTMK010000291         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_526592, whole genome shotgun sequence.
ACCESSION   DASTMK010000291 DASTMK010000000
VERSION     DASTMK010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526592"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="VFQ79_12450"
     CDS             <1..1753
                     /locus_tag="VFQ79_12450"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEU0140523.1"
                     /translation="KEKNAGIRQRVVPLLAPMTKHRHSDGSYQTKARGLALLNTPRLN
                     KGTAFTAEERTSLGLTGLIPPDISTLEAQVERAYIQYERLPDALSKNIYLTALHDRNE
                     VLFYRLFSEHLREMIPIVNDLTVGMAMEQYHHECRPPRGVYLSIDHHDGIKEAFANLG
                     AGAGEIDLIIATDAEQILGIGDWGVGGIEVPIGKLAIYTAAGGVDPTRVIPVMLDVGT
                     DRQSLLDDPTYVGNRHSRVRGARYNTFIDTYVAAVTELFPHALLQWEDFAPANGRRIL
                     EKYRDRICTFNDDMQGTGAITLAAAISAVRVCGTPLRNHRVLIFGAGTAGIGIADQIR
                     GAMVREGLSPGEAIDRFWCVDRHGLITSDMTAHIGEYQAAYARPAAESKNWRHGGNGS
                     GIGLAEVVRRVKPTMLIGASTAPGSFTEGVVKEMAAHVERPIIFALSNPSARAEANPA
                     DLITWTEGRALVATGSAFAPVTYKGVTYAVAQVNNAMLYPGLGLGAIVARAGRISDGM
                     FAAAAGAVSSLVAVRQPGASLLPHIDDLRTVSATVAMAVVEAAAAEGLATIKVGDIVQ
                     QVQDAMWQPEYRRIEAS"
     gene            1773..>3104
                     /locus_tag="VFQ79_12455"
     CDS             1773..>3104
                     /locus_tag="VFQ79_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0140524.1"
                     /translation="MNEPPKIQKIAFIGDHLPRKCGIATFTSDLLAAVQSEHPESRCL
                     AVSVNDIQDGYEYPEVVRFEVEEQDLPSYLRAADFLNISNVDVVCLQHEFGIFGGPAG
                     GHILALLRELRMPVVTTLHTILREPRADQRRVMEGLISLSTRLVVMAERGRQMLHEIY
                     QAPVTKIDLIPHGIPDVGFVDPTYFKDQFGVEGKVVLLTFGLLSPNKGVEHVLNALPQ
                     ILAEFPEVVYIVLGATHPNEARERGEAYRLSLEILARKNKVEKSVIFYNQFVDLDNLK
                     EFIGAADLYVTPYLNEAQITSGTLAYTFGSGKAVVSTPYWHAAELLADDRGVLVPFGD
                     APAIAREVIALLRDDTRRHAIRKNAYKIGREMVWSNVAQMYMRAFECSRREVAAFPRK
                     FLGARTLDKKPRELPPIKLNHLQRMTDSTGVFQHAAFSVPNFSEGYCTDDNA"
BASE COUNT          620 a          937 c          965 g          582 t
ORIGIN      
        1 gaaagagaag aatgctggta tccggcaacg agtggtgccg ctgttggccc ctatgaccaa
       61 gcaccgacac agcgacgggt cctatcagac caaggcgcgg ggcttggccc tgctcaatac
      121 gccgcggctg aacaagggaa ctgccttcac ggcggaggag cgaacgtcgc tggggctcac
      181 cggtctgatc ccacctgaca tcagcaccct ggaggcccag gtggagcgcg catacatcca
      241 atacgagcgg ctcccggacg cgctgagcaa aaacatctac ctgaccgctc tgcatgatcg
      301 gaatgaggtg ctgttctatc gactgttctc cgagcacctg cgcgaaatga tccccatcgt
      361 caacgatctt accgtcggaa tggctatgga acagtaccac catgaatgcc ggcctccacg
      421 cggtgtgtat ctgtccatcg atcatcatga cggcatcaaa gaagcgttcg ccaacctcgg
      481 agccggcgcc ggagagatcg acctgatcat tgcgacggac gccgagcaga tcctggggat
      541 cggcgattgg ggcgtaggcg gcatcgaggt cccgattggc aaactggcaa tctatacggc
      601 agccggggga gtcgacccca cccgagtcat ccccgtgatg ctcgacgtcg gcacggatcg
      661 gcagagcctc ttggacgacc cgacctatgt aggcaaccgg cactcgcgag tccgcggggc
      721 gcgctacaac accttcattg atacttatgt cgcagcggtg acagagctgt ttccgcacgc
      781 gcttctgcaa tgggaggact tcgcgcccgc aaacggacgc cggatcctcg agaagtaccg
      841 cgaccgcatc tgcacgttca atgacgatat gcagggcacc ggcgccatca cactggcggc
      901 tgcgatctcg gcggtccgcg tgtgtggtac gccgctgcga aaccatcgag tcctgatatt
      961 cggcgcgggt acggcgggta tcggaattgc cgaccagatt cgcggcgcca tggtgcgcga
     1021 gggcctgtcc ccaggggagg caattgaccg cttctggtgc gtggaccgtc atggcctgat
     1081 caccagcgat atgacggcac acatcggcga ataccaggcg gcgtatgccc ggcccgccgc
     1141 ggaaagcaag aactggcgcc acggcggaaa cggaagcggt attggcctgg ctgaagtggt
     1201 gcgccgggtc aagccgacca tgctgattgg cgcctcgacc gccccaggca gcttcacgga
     1261 gggggtcgtc aaggaaatgg cggcgcatgt ggagcggccg atcattttcg cgctctccaa
     1321 tccatcggca agggcggagg cgaaccccgc cgatctgatt acctggaccg agggtcgggc
     1381 gctcgtcgcc accggcagcg cgttcgcgcc ggtcacctac aagggggtga cgtatgcggt
     1441 cgcccaggtg aacaatgcca tgctttaccc cggcctcggc ctcggcgcga tcgtcgcgcg
     1501 ggccggaagg atcagtgacg gcatgttcgc cgcggccgcg ggcgccgttt ccagcctggt
     1561 ggctgtgcgt cagcccggtg cgtcgctcct tccgcacatt gacgatttgc gcaccgtatc
     1621 cgccacggta gctatggccg tagtggaggc cgcggcggcg gagggccttg ccaccatcaa
     1681 ggtcggcgat attgtgcaac aggtgcaaga cgcgatgtgg cagccggaat accgccgtat
     1741 tgaggcgtcc tgaggggatc gcgaaatctg ttatgaatga accgcctaaa attcagaaga
     1801 tagccttcat cggcgatcac ctgccgcgca agtgtggcat cgccacgttc acctctgatt
     1861 tgcttgccgc agtccagagt gagcatccgg agagccgctg cctggcggtg tcggtcaacg
     1921 atattcagga tggttacgag tacccggagg tagtgcgttt cgaggttgaa gaacaagacc
     1981 tgccgtccta tctacgcgcg gcggacttcc tcaacattag caacgtggac gtggtgtgcc
     2041 tgcaacacga gttcggtatt ttcggaggtc cggccggcgg tcacattctg gcgcttttgc
     2101 gcgaactgcg aatgcccgtc gtcaccacgc tacacaccat cctgcgtgag ccgagggccg
     2161 accagcgccg cgtcatggaa ggtctgatct ccctctcgac ccggctcgtc gtgatggccg
     2221 agcggggcag gcagatgctg cacgagattt accaggcgcc cgttaccaag atcgatctga
     2281 taccccacgg gattcccgat gtcggcttcg tcgatcccac ctacttcaag gaccagttcg
     2341 gcgtggaggg gaaggtggtg ctacttacct ttgggctgct ttcgccgaac aagggggtgg
     2401 aacacgtcct gaatgccctg ccgcagattc tggcggagtt tccagaagtc gtttacatcg
     2461 tcctgggcgc tacccatccc aacgaggctc gcgagcgtgg tgaggcctac cggctcagcc
     2521 tcgagattct cgccaggaag aacaaagtcg aaaagagcgt catcttctac aaccagttcg
     2581 ttgatttgga caatctcaaa gaattcattg gcgcagcaga tctctatgtg acgccctatc
     2641 tcaatgaggc gcagattaca tcgggcacgc tggcgtacac gttcggttcg ggcaaagcgg
     2701 tggtttcgac gccatactgg cacgcagccg aattgttggc ggatgaccgc ggtgtcctgg
     2761 tgccattcgg cgacgcgccg gccatagcac gcgaggtaat agcgttgctc cgcgacgaca
     2821 cgcgcaggca tgctattcgc aaaaacgcct acaagattgg ccgcgagatg gtttggagca
     2881 atgtggccca gatgtacatg cgtgctttcg agtgctcccg gcgcgaagta gcggcatttc
     2941 cgcggaagtt cctgggcgcc aggacgctcg ataagaagcc ccgtgaactg ccgcctatca
     3001 aactgaatca tctgcagcgc atgaccgatt cgaccggagt cttccagcac gccgccttca
     3061 gcgtcccgaa cttttccgaa ggctattgca ccgacgacaa cgcg
//
LOCUS       DASTMK010000292         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_333801, whole genome shotgun sequence.
ACCESSION   DASTMK010000292 DASTMK010000000
VERSION     DASTMK010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333801"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /gene="gndA"
                     /locus_tag="VFQ79_12460"
     CDS             complement(<1..1237)
                     /gene="gndA"
                     /locus_tag="VFQ79_12460"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR00873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU0140525.1"
                     /translation="METDGYEIGMVGLGVMGRNLLLNMADHGFPVAGYDKDAGKVEAL
                     QNESAGKKIKAVANVQEFVGLLRRPRAIMMLVPAGPPVDSVIGDLLPHLEKGDVLIDA
                     GNSHFKDTNLRTGTLSERNISFLGVGVSGGADGARHGPSIMPGGPRAAYERVRPVLEA
                     VSAKVSEEPCVAYLGPGSAGHFVKMVHTGIEYAMMRLIAETYDVMKRGLRMSDNELGG
                     VYDTWNKGELNGYLVEITSRIFSRVDERTGKPLIDQILGVARQKGTGMWTSQSAMELQ
                     VPIPTIDLAVSMRDMSVFEKQREAASRIFGRAIPPFNGDRGAFVAQLGHALYAAMIIA
                     YAQGMMVLAAASEQYGYQLNLEAVARIWRGGCIIRAALLEDIRMAYRQRPDLPNLLLS
                     PELSKKVLGYDEDLRLVARA"
     gene            complement(1262..>1531)
                     /locus_tag="VFQ79_12465"
     CDS             complement(1262..>1531)
                     /locus_tag="VFQ79_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140526.1"
                     /translation="DQQVFEVQVDLDKVDPNAVRVELFADGVNGGDPVRQPMERVRRM
                     QNAGAYVYRTALPLARPATDYTVRLMPQFDGVAIPLEANRILWQR"
BASE COUNT          294 a          508 c          415 g          314 t
ORIGIN      
        1 ccgcgcgggc gacgaggcgc aagtcctcgt cgtatccgag taccttcttc gatagttccg
       61 gagacagtaa cagattcggc aaatcgggcc gctggcgata agccatccgg atgtcctcaa
      121 gcaacgccgc gcggatgatg cagccacccc tccagattcg tgctaccgct tccagattaa
      181 gctggtatcc atactgctca gacgccgcgg ccagcaccat catgccttgt gcgtaggcga
      241 tgatcatcgc agcgtaaagt gcgtggccca gttgggcgac gaaggctccg cggtcgccat
      301 taaagggcgg tatcgctcta ccgaagatcc tgctggctgc ctcgcgctgc ttctcaaaca
      361 ccgacatgtc gcgcatcgac acagcgaggt cgatggtcgg gatgggaacc tgcagttcca
      421 tggcgctttg cgaggtccac atgccggtgc ccttctgcct ggcaacgccg aggatctgat
      481 cgatcaacgg cttgcctgtc cgctcgtcta ccctgctgaa gatgcggctg gttatttcta
      541 ccagataccc attgagttcc cctttgttcc atgtgtcgta aacaccgcca agctcgttgt
      601 cgctcatgcg aagaccgcgc ttcatcacgt cgtaggtctc ggctatcagc cgcatcatcg
      661 cgtactcgat tccggtgtgg accatcttca cgaaatgccc cgcggaaccg gggcccaggt
      721 aagccacgca cggctcttcg ctgactttgg cggagacagc ctcgagcacc gggcgcaccc
      781 gttcgtatgc cgctctcggt cctcccggca tgatgctcgg gccgtggcgt gcgccgtccg
      841 ctccaccgga tacccccacg ccgagaaatg aaatgttacg ctcgctcagg gtgccggtac
      901 gcagattcgt atctttaaag tgggagttcc ctgcgtcgat aaggacgtcg cccttttcga
      961 gatgtggcaa cagatcaccg atcacggaat ccaccggtgg gcccgcgggc accagcatca
     1021 tgatggcgcg cggccggcgg agcaatccga caaactcctg gacgttggcg acggccttga
     1081 tcttctttcc cgccgactca ttctggagcg cttccacttt gccggcgtcc ttgtcgtagc
     1141 cggctaccgg gaagccgtgg tcagccatgt tcagcaacag gttgcgcccc atcacgccca
     1201 ggcccaccat cccaatttcg tagccatccg tttccattcc aaccctccct cccaagtccc
     1261 atcaacgctg ccacagaatt cgattcgctt cgagaggaat agcaacgccg tcgaattgcg
     1321 gcatcaggcg caccgtgtag tccgttgccg ggcgggctaa aggcaacgcc gtcctgtaaa
     1381 cgtaagcgcc agcattctgc attctgcgaa cccgctccat gggctgccga acagggtcgc
     1441 caccgttaac gccatcggca aaaagctcca ctcgcaccgc attcgggtcg accttatcga
     1501 gatccacctg aacctcaaac acctgctggt c
//
LOCUS       DASTMK010000293        12930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_429799, whole genome shotgun sequence.
ACCESSION   DASTMK010000293 DASTMK010000000
VERSION     DASTMK010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12930
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429799"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFQ79_12470"
     CDS             complement(<1..870)
                     /locus_tag="VFQ79_12470"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0140527.1"
                     /translation="MGLRQFVLSLLVIATTASVAWSQASTATVSGTVRDQTGAVIPGA
                     AVTLTNRNTNVGSKATTNEVGFYVFPGTLPGPYLLAVEAGGMQRFEGSLTVQVQQSAV
                     VDVAMRVGQTATEVAVVDVTPMIQVDNPTLGHVLERARIEQLPINGRNVTSLLQTVPG
                     MEGTRAYGLREGSHELVLDGAALTDRLWGGTVRRQPGLDTIQEFKVENNSSSAKFTRP
                     TTMIMTTKSGTNQLHGAAFWTNRNNSIGKARSRTDNYTKAPFLNRNEFGVSSGGPVYI
                     PKLYNGKDRTFWFF"
     gene            1555..2913
                     /locus_tag="VFQ79_12475"
     CDS             1555..2913
                     /locus_tag="VFQ79_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140528.1"
                     /translation="MRLRIMSGLLLALVALPQATEELFITSEHPRLLLNSRRLKLLRR
                     ERVRESIRWQQFQTLMTGGVPMPEPGFANALYYRIADDAEAGRRAVEWALAPQTDLRQ
                     LAIVFDWCQPLLDNNQSAAIAARLRAGLDAATGARDLPAVRSAVMAAIALAGHNPDAE
                     RLLNELIRKKWQEDLTPKLGAQPVPFPLHETYALYELIHTLRDNTGVDLRDSARAFFK
                     TFPAYHMLAHYPASYPAGENDFRIPVSDAEKEPDLRRASLSRAAELSMVAYDTNAVES
                     QFVQGWLINDRFLMRGPFGAPYEFLWANPYQPGLSYFHMPLVFHDPATGRLILRSSWE
                     EDAQWFGHLEGWTQLFENGRIVKVRTRTKQPPVRMGEATIVFAASGLRFRGGGDNGSE
                     LFVVGLTPSREYEIEIDDREMFEQTADAGGILSISISKGAVAGVRIREVAQPGQSPRN
                     RP"
     gene            complement(2889..4079)
                     /locus_tag="VFQ79_12480"
     CDS             complement(2889..4079)
                     /locus_tag="VFQ79_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0140529.1"
                     /translation="MSTLTPAVPTEPGQAGRSIGTIVIALAAVIALLYFGRVFIITLI
                     IATMLAFILEPAVSFLVRFRFPRGAASFMVCSVALLLIYLTGLGLYTQISGLLEELPS
                     YSQRISDLVDNVSSRLEAAEKSLYEAMVPKRIRERDQLAAEAMAQQAAQAAAKKTRGR
                     KAPPPEPSPPTQQVPEVRIQEQRTPLVNYLYSYLSSFYNILLMASFVPFLVYFMLSWR
                     DHIRRSFLQFFDGQARLIAGKSWHGIAEMARAYIVGNFVLGLLLSMASWLFFISMSLP
                     YPFLIAPFSGFLSLVPYVGLPLAMVPPLLAALPVYDALTPYLIIGTTIAVLHLLALNL
                     LYPKIVGGRVHLNPLVVTVALMFWGTLWGGIGLVLAIPITAGIKAVCDNVVELRPYGR
                     FLGD"
     gene            4149..4613
                     /gene="ruvX"
                     /locus_tag="VFQ79_12485"
     CDS             4149..4613
                     /gene="ruvX"
                     /locus_tag="VFQ79_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU0140530.1"
                     /translation="MDLASGGRILALDLGKRRIGIAVSDALRMTAQGLDTLVRTNIRS
                     DILALEEIIRSRDVSLVLIGNPLHMSGHEGAGSAFARDFAERLSAHTGVAVRLWDERL
                     TTVEAQRVLRQSGASIGKRARAVDKLSAVILLESYLDSLAGSDGEMPADATF"
     gene            4600..5598
                     /gene="mltG"
                     /locus_tag="VFQ79_12490"
     CDS             4600..5598
                     /gene="mltG"
                     /locus_tag="VFQ79_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU0140531.1"
                     /translation="MRLSKLLLPAFVLLLLAAGALAAALLVPYSGLGKETFVDIPKGT
                     DTRHMATLLRQAGVIRFDWQFLLARAIRKDARLQAGEYRFHTPASVWTVFDRIVRGDI
                     FYHQVRVPEGHNIFDIANTLENQKLIPSEAFLKVARDPALIQDLDPQAPSLEGFLFPS
                     TYRIARHTTPEQVCRMMTDAFRRVWKELDPAPETDIHTIVTLASLVEKESAVPQERPQ
                     VASVYTNRLRKGMRMDADPTVIYAALLENRYRGSIYRSDLDKDHPYNTYRHAGLPPGP
                     IANPGRGALEAALRPAETEFLFFVAKPDGSGAHEFTTDLTEHNKAVLNYRRGAPQP"
     gene            5579..6067
                     /locus_tag="VFQ79_12495"
     CDS             5579..6067
                     /locus_tag="VFQ79_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140532.1"
                     /translation="MARRNPKRDRIAEAIRESGWERIGVEEFARLQAAFVPVSARYLR
                     EAIRAAGVPMSPLAEGIRQDSLEELERTLLRMQTEYQAANQAGDARRARQCRAAVIKS
                     KDHAKWALKKAVQEEKRRERAEMILWMLTWLENPALFPDWLKLRKREVRRETVPPLRI
                     SY"
     gene            complement(6061..6381)
                     /locus_tag="VFQ79_12500"
     CDS             complement(6061..6381)
                     /locus_tag="VFQ79_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140533.1"
                     /translation="MRFLRYAGAIAVFSLAAFADTFTGTVVDVMCKDKDLASHTRKCA
                     IGCAKSGYGLMTADGKFMKFDEEGNAKTLAALKASSKEKDLKATVEGTREGETIKVDS
                     IELQ"
     gene            6755..7021
                     /locus_tag="VFQ79_12505"
     CDS             6755..7021
                     /locus_tag="VFQ79_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140534.1"
                     /translation="MIGRDNFDALTPQKEAAMFYGLFLRGHSVDALRKDIDIPRATLD
                     KWLNTPRFEPDFQDNLKRVYAYRKQVLAIFDQLVTHELKKPRLH"
     gene            complement(7079..9823)
                     /locus_tag="VFQ79_12510"
     CDS             complement(7079..9823)
                     /locus_tag="VFQ79_12510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0140535.1"
                     /translation="MSWTDLILRLRSLVRYRSAEGELDEELSFHMEMEARKNRAAGMA
                     DTEARRSARARFGGIEQVREQCREVRGLTFLEDMVRDIRYGARILRKAPVFTAIAVLS
                     LAIGIGANTAVFSLLDTVLLRMLPVQNPEQLVVVRWGAKKNLNLSSTWASGRGGSGGW
                     TTNVFSWEIFSGIRVRSQTLGSIMGFSPLGPVNVAVNRQALAAGAMVVSGNYFRALGV
                     GTVLGRPITEDDDTADGLTSAVISYRFWERMFALDPSAIGKTLYVNGQPCIVIGVTPR
                     EFFGVSAGGFMRTPGVDITLPIRSRERMQGSGHQRVNWFGDDLFWVQVMARLNQEKEE
                     TAAGNELAAIVSAGLPETARSIMGSETPRIFQEPGGQGLDSLRRVYRRPLLILMVVVA
                     LTLLMACANLAGLLLARATARQREIMLRLAVGASRWRLVRQLLVEGALLSAAGAIAGV
                     AFAYWGVRALLALLASGSTPIPLAVSPDARVLAFTVAVSLAATFLFALAPAIRATRVD
                     VASGLKEDTPAVRMRRFGGGPILVAVQVAVAVVLLAGATLFTRSLANLRSLSLGFNPY
                     NLVLFDLSPGKNGYDENRGNQLYARVLERLKQIPGVTGASLSAQRLVSGFMSNGSVLV
                     EGAASEKPVRSTFNFVGPEFFEVMGIPVIMGRGFELRDMGATPRVAVINETLALQHFG
                     SGSPVGRKFRWSFKKEWDVEVIGVAKNAKYDRLRDDAPATIYTPYTQAPFGWPQEMSF
                     EVRTAASAGDVVARIRRAIAEIDGMLPLTGVKTQQAQIDDTLAQEHLFASLVSLFSAI
                     TLVLVCVGLYGSVSYAVTRRTRELGIRMALGARKLAVMRMLLGQVTFAIAAGLAAGLP
                     LTWMLTRIIESQLYGIKPHDPVSLLVASAGVTGVSILAAFLPARRAMRIDPVRALRYD
                     "
     gene            complement(9825..10160)
                     /locus_tag="VFQ79_12515"
     CDS             complement(9825..10160)
                     /locus_tag="VFQ79_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0140536.1"
                     /translation="MNRTPKLDLLQGTLDLLILRTLLAGPQHGWAISERIQQVSADVL
                     RVNQGSLYPALHRLEHQGSIKAEWGISELGRRARFYRLTAAGRKQLEVETEAWTRMAG
                     AIGRVLEMA"
     gene            10184..10342
                     /locus_tag="VFQ79_12520"
     CDS             10184..10342
                     /locus_tag="VFQ79_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140537.1"
                     /translation="MALLLSDVNNKNAGRPVYFLVSFFPVFGDLPESSNEETCTPIFR
                     HNLIAGER"
     gene            complement(10458..>12930)
                     /locus_tag="VFQ79_12525"
     CDS             complement(10458..>12930)
                     /locus_tag="VFQ79_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEU0140538.1"
                     /translation="DVFLRVQENNANFGGGFIEHYAEQYTVRGLGRTQSIGDVSNIVV
                     LARAGTPVLLRDIAHVTIAPMQRQGAVLRDGQGETVSGMAIMLKGENGRRVIDRVKAK
                     LSSIRLPEGVKIVPFYDQSEVIEGTIGTVRKNLMEAGVLVVAVLLLFLGNIPAALIVA
                     SVIPLSLLVGFIGMAVFGISANLMSLGALDFGMIVDGAVVMMENSVRKLERKPGEKIA
                     NVLGRVRHAAHEVARPIVFAVAIIIAVYMPIMFLEGLEGRMFRPMAITVCSALVGALV
                     FSLTVVPTAAAIVLRKGVKPHTGGWFSKVRRSYEATLRFTMRHRLAAVLTGLALVTVS
                     LASLAYIGTEFMPRLDEGSLLIQTRKLPGISLTESIELSDEIEKVVMKFPEVTGVVSK
                     LGRPDMATEAMGIYEADVYVRLKPVREWTTAETKEELFEKMSHSLEKVPGVAYSFTQP
                     MAMRLDEVVSGIKADVALKIFGEDPRVLEQLAERAQTLMATVPGAADLQMEILSGVGE
                     LRVDVDRTALARYGLDITRVRELVEAAVAGKYVSEMIEGQRRFPIVIRFPERYRADAE
                     SLRQLLIAAPAGERVPLGSVARVEVVRGPEVVSREDGQRRIVVQANVRGTDLGSFVAE
                     AERKLETGLVLPAGYSTDWGGQFENQQRAMQRLAIVLPLTVLIIFALLFATFDSVPQA
                     ILIILNVPFALVGGIGALWLRGLNLNLSASIGFLALFGVAVLNGIVLVSYINRLRADG
                     KPLHDAILEGAGTRLRPVLMTALVASLGFVPMATSTSIGSEMQRPLASVVIGGLVTST
                     LLTLYMLPLLYPWACREASRENQAE"
BASE COUNT         2776 a         4015 c         3664 g         2475 t
ORIGIN      
        1 aaagaaccag aacgtcctgt ccttgccgtt gtacagcttg ggaatataaa ccggacctcc
       61 actgctcacg ccaaactcgt ttcggttcag aaatggcgct ttggtgtagt tgtcggtcct
      121 ggaccgggcc ttgccgatag agttgttgcg gttcgtccaa aaggccgcgc catgaagttg
      181 gttggtaccg ctcttcgtcg tcatgatcat cgtcgtggga cgggtgaact tggcggacga
      241 gctattattc tcgactttga actcctggat cgtatccagg ccaggctgcc ggcgtaccgt
      301 acctccccat agccggtcgg ttagcgcggc gccgtcgagc accagttcgt gcgagccctc
      361 acgaagacca taagctcgcg ttccctccat cccgggaacg gtctgtagca atgaggtgac
      421 attgcgcccg ttgatcggca actgctcgat tcgagctcgc tccaggacgt gtccgagagt
      481 ggggttatcc acctggatca tcggcgttac atcgactact gcaacttccg tggctgtttg
      541 tccgactctc atcgcgacat ccaccaccgc gctctgctgc acttgcacag tgagagagcc
      601 ctcaaacctc tgcatccccc cggcttccac agccaggaga tagggcccgg gcaaagtgcc
      661 cgggaaaacg tagaagccga cttcgttcgt cgtcgctttg gatcctacgt ttgtgttccg
      721 gttggttagc gtgaccgctg ctcctggaat gacagcgccg gtttgatcgc gcactgtccc
      781 actcaccgta gctgtgctgg cttgagacca tgctacagag gcggtcgtcg cgatcacaag
      841 aagagacaga acgaactggc gtagacccac tcaaaccctc ctgagattct aatgggttag
      901 attttgtact gttttgtctt cgattgtcaa gcggtaagaa gttaattttg agttagttcc
      961 aggggttcta gtcctacccg gttagtaaga agattgggga agcctgacga gaagaaccgc
     1021 accggatcgg gcggtgaagt gccctctccg gcgcggcccg ccccactgac aggggctggg
     1081 atgcagcgtc ggtacgtccg gttatcctat atacccgcaa acgttcattc ctggtgaggc
     1141 ccgcgtccaa tctccccact cagcaatgcc agacgcatac cgaaacgtgt catccacggt
     1201 gagtccgtaa gtactggtaa ctaatcccgg taatgccgcg aatccgaagt aacctaaaaa
     1261 aaaccctctg ttgcaagccg caacccgaaa gaaggagcgg tttgaattct tccacaccgg
     1321 ctcccgcaag cacgttgctg ttgagcccgc tcgctctccc cgcttgccgc gttgcagcag
     1381 agggcaggtt aagggaacta ccatcaattc gtccgttaag actgcaaacc actggccagc
     1441 ttcgtccggc agataagtta ttgaaataga aaactcagac cgttcggttg caggcgagag
     1501 ccgggaccgc agagtgcgcc gggtgtgtca gtgtgacaca atcggaacag ccggatgcgc
     1561 ttgaggataa tgagcggttt gctgttggcg ctggtggcct taccgcaagc cacggaagag
     1621 ttgttcatta ccagcgagca tccgcgactc ctgctgaatt ccagacgcct gaaactgctg
     1681 cggcgggaac gggtgcggga atccatccgc tggcaacagt tccagacttt gatgaccggt
     1741 ggggtcccga tgccggagcc gggctttgcc aatgctttgt actatcgcat cgccgatgat
     1801 gcggaagcgg ggcggcgtgc cgtggagtgg gcactggctc cccaaacgga cctgcgccag
     1861 ctcgcaatcg tatttgactg gtgccagccg cttctcgata acaaccaatc ggcggccatt
     1921 gccgcccggc ttcgcgctgg gctggatgcg gcaacgggag cgcgggacct gccggcggtg
     1981 cgttccgccg tcatggcggc catcgccttg gcaggtcaca acccggatgc ggagcgtctt
     2041 ctcaatgaat tgatccggaa aaaatggcag gaggacctca cgccgaagct tggggcgcaa
     2101 ccagtaccgt ttccccttca cgaaacctat gcgctctatg agttgattca caccttacgg
     2161 gacaacaccg gcgtggacct gcgcgattcg gcgcgcgcat tcttcaaaac ttttccggcg
     2221 tatcacatgc ttgcacacta tccggcttcg taccccgccg gcgagaacga tttccggata
     2281 ccggtatccg atgcggaaaa ggaacccgat ctcaggcggg cctccctttc ccgggcagcg
     2341 gagttaagca tggtcgccta tgataccaac gcggtcgaga gccagtttgt gcaaggctgg
     2401 cttatcaacg accgttttct catgcgaggt cctttcggcg cgccttacga gttcctttgg
     2461 gccaatccct accagcccgg gctcagctac ttccatatgc cgctggtctt ccacgatccc
     2521 gccacgggcc gcctgattct gcgttcgagc tgggaggaag atgcccaatg gtttggacat
     2581 ctcgaaggat ggacgcagtt gttcgagaac ggccgcatag tgaaggttcg aacccggacc
     2641 aaacagcccc ctgtacggat gggtgaagcg acgatcgtgt tcgccgcgag cgggctgcgg
     2701 ttccggggtg gcggcgacaa cggatccgaa ttattcgtcg tcggccttac tccttcgcga
     2761 gaatacgaga tcgagataga cgacagggag atgttcgagc agactgcgga cgccggcggc
     2821 attctctcca tttccattag caagggagca gtcgccggcg tacgaatccg tgaagtggcc
     2881 cagccgggtc aatcgcccag gaaccggcca taaggacgta gttccactac gttgtcgcag
     2941 acagccttga ttcccgccgt aatgggaatg gccagtacca gcccgatgcc tccccacaga
     3001 gtgccccaga acatcaacgc cacggtcacc accagtgggt ttaaatgcac tcgcccgccg
     3061 acgattttcg gatacagcag attcagcgca agcagatgga gaaccgcgat agtcgttcca
     3121 atgatgaggt aaggcgtcag cgcatcgtat accggcaggg cagccagcag cggaggcacc
     3181 atggcaagcg gcagcccaac gtaaggtacc aggctgagga agccgctgaa tggtgcgatc
     3241 aggaatgggt agggaaggct catgctgatg aagaaaagcc aactcgccat cgaaagaagc
     3301 agaccgagca caaaattacc aacaatataa gcgcgcgcca tctccgcgat gccgtgccag
     3361 ctcttgcctg caatgagacg tgcctgtccg tcgaagaact gtaaaaaact ccggcggata
     3421 tggtcgcgcc agctcaacat gaaatacaca agaaacggca cgaacgacgc catcaggaga
     3481 atgttataaa acgaacttaa ataggaatac agatagttga ccagcggcgt tctttgctcc
     3541 tgaatgcgca cttccggaac ctgctgcgtg ggcggagacg gctctggagg cggggccttg
     3601 cgccctcgcg ttttcttcgc ggccgcctgc gcagcctgct gcgccatcgc ttccgcggcc
     3661 agttgatcgc gttcgcgaat ccgtttggga accatggcct cgtacagcga tttctccgcc
     3721 gcctccagcc gcgaggagac gttatccact aaatcgctga tgcgctggct atagctggga
     3781 agttcctcca acaggcccga aatctgtgtg tataaaccga ggcccgtgag ataaatcagc
     3841 agcagagcca cactgcacac catgaaactg gcggctcctc ggggaaaccg gaaacgaacc
     3901 aaaaagctga ccgccggctc cagaataaag gccagcatcg tggcaataat cagcgtaata
     3961 ataaatacgc ggccgaagta gagcagtgcg attacagccg ccagcgcgat aacaatcgtg
     4021 ccgatactgc gccctgcctg ccccggttcc gtcgggaccg caggagtcaa agtggacacc
     4081 gaggaaacct ctcaggagac ctttaccagc tactctagca tgaagaccgg tagaatatta
     4141 gcagtactgt ggatctggct tcaggcggca gaattctcgc tctcgacctc ggtaaacggc
     4201 gcatcggaat agctgtcagc gatgcgctgc gaatgaccgc gcaagggctc gatacgctgg
     4261 tgaggacgaa tatccgaagc gacattctgg cacttgagga aattatccgt agccgtgatg
     4321 tctcgctggt attgattggg aatccgcttc atatgagtgg gcacgagggg gcggggtccg
     4381 cgttcgcacg ggactttgcc gaacggctgt ctgcgcatac gggagtagct gtccggcttt
     4441 gggacgaacg cctgacgacc gtggaagcgc agcgtgtgct tcgccagagc ggcgccagta
     4501 tcgggaagcg cgcccgggcc gtggacaagc tgtccgcggt gattctgctc gagagctatc
     4561 tcgattcgct cgctggaagc gacggggaga tgccggccga tgcgactttc taagttgctg
     4621 cttccggcgt ttgtactcct tttgctcgcc gccggagcgt tggccgctgc tctcttggtg
     4681 ccgtactcgg gtttaggaaa agagacattt gtcgatatcc cgaaaggcac cgatacccgc
     4741 cacatggcca cgttgttgcg gcaggccggt gtcatccgat tcgactggca gtttctgctg
     4801 gcccgcgcga tccggaaaga tgccagattg caggcgggcg agtaccgctt ccatactccc
     4861 gcttcggtct ggaccgtatt cgaccgcatc gtcaggggcg acatcttcta tcaccaggtg
     4921 agggttccgg aaggtcataa tatcttcgac atcgcgaaca cgctggaaaa tcagaagctg
     4981 attccctcag aagcatttct caaggtggct cgcgacccgg cactgattca agatctggac
     5041 ccgcaggcac cctcgctcga aggttttctg ttcccttcca cctaccgcat cgcccggcac
     5101 actacgcccg agcaggtatg ccgtatgatg acggacgcgt tccggcgtgt atggaaagag
     5161 ctggatccgg cgcccgaaac ggacattcac acgattgtca cgctcgcgtc gctggtggag
     5221 aaggaatccg cggttccgca ggaacgcccg caggtggcgt ccgtatacac gaatcgttta
     5281 cgcaagggga tgagaatgga cgccgacccg actgttattt atgccgcgct gctggagaac
     5341 cgttaccgcg gctccatcta cagatccgat ctggataagg atcaccccta caatacctac
     5401 cgccatgccg gactcccgcc cgggccgatt gccaacccgg gacgcggtgc cctggaagcg
     5461 gcgctgcggc cggccgagac cgaattcctg ttctttgtcg cgaagccgga cggctccgga
     5521 gcgcacgaat tcaccacgga tctcaccgaa cataacaaag ccgttttgaa ctaccgccgt
     5581 ggcgcgccgc aaccctaaac gcgaccggat cgccgaagcc atccgggagt ccggctggga
     5641 gaggatcggc gtagaggagt ttgcccggct tcaagccgca ttcgtgccgg tttcggctcg
     5701 atacctccgc gaagccattc gcgcggccgg cgttccgatg tcccctctgg ctgagggcat
     5761 ccggcaggat tcgctggagg agttggagcg cacgctgcta cgaatgcaga ccgaatacca
     5821 ggcggccaat caagcaggcg atgcgcggag agcccgccaa tgccgggcag cagtcatcaa
     5881 atctaaagac cacgcgaaat gggcactgaa aaaagctgtg caggaggaga agcggcgcga
     5941 acgcgcggag atgattttat ggatgttgac ctggctggaa aacccggcct tgttcccgga
     6001 ctggctcaaa ctgcgaaagc gtgaagtgcg gcgggagacg gtcccgccgc tccggatttc
     6061 ctactgaagc tcgatgctat cgactttgat ggtttccccc tcccgggtac cttctaccgt
     6121 cgccttcaaa tctttttctt tggaacttgc tttaagggcg gccagcgttt tcgcgttgcc
     6181 ctcttcatcg aacttcatga acttgccatc cgcggtcatc aaaccgtaac cgctcttggc
     6241 acaaccgatg gcgcactttc tcgtgtggct cgccaggtcc ttgtccttac acatcacgtc
     6301 cactacggtg cccgtgaaag tgtcagcgaa agcggccagc gagaatacgg cgatggcgcc
     6361 ggcgtacctc aggaaacgca tatggaaaca tccctcctct gagaaaatct actcatagca
     6421 tgacacggac ggagccgggt taccagatcc attctgtcgt cagacgcccg atcctcagcg
     6481 cggcaacact ctaaaatctt gatttcactc gtttgaaacc gccataaaga ccgccgatgt
     6541 taaaatatcg tagcgttccc accccaggcg ctgtaaaagc tcttgaaact gggatatcgc
     6601 gcgacgcatc tgattcactt gaaggtcgcc gcaagtgtat cctccgcgtt aatcgtggtg
     6661 agaaacaatg gtcgtttcgg tacttgattg tatggggcgg cctgattata ctggcttttt
     6721 ttgaccggac aattcttctg agtgagtagt ctagatgatt ggaagggaca attttgacgc
     6781 actgacgccg cagaaggaag cggcgatgtt ttatgggttg ttcctgagag ggcattctgt
     6841 tgacgccctc cgcaaggaca tcgacatccc ccgggctacg ctcgacaaat ggctgaacac
     6901 gccccgtttc gagccggatt tccaggacaa tctcaagcgg gtttacgcct atcggaaaca
     6961 ggtgctagcc atcttcgatc aacttgtgac gcacgagctc aagaagccca ggctccacta
     7021 accccttcca ccccttgcca gcaagacaca tacgccccgg ttcgggccgt catacgtttc
     7081 aatcgtagcg cagggcacgc accggatcga tacgcattgc gcggcgcgca ggcagaaaag
     7141 ccgccagaat agagactccc gtcacacccg ctgacgcaac cagcaggctg accgggtcat
     7201 gcggcttgat gccatagagc tgcgattcga tgatacgagt caacatccat gtcagcggca
     7261 ggccggctgc gagaccggcc gcaatggcga atgtcacttg gccaagaagc atacgcatca
     7321 cggcaagctt gcgggcgccc agcgccatcc ggatcccgag ttcgcgcgtg cggcgggtca
     7381 ccgcgtagga taccgatccg taaaggccca cgcagaccag caccagcgtg atcgcgctga
     7441 acaggctcac cagcgaagca aacaggtgtt cctgcgccag ggtgtcgtca atctgcgcct
     7501 gttgcgtttt caccccggta agcggaagca tgccgtcgat ctccgcaata gcgcgccgga
     7561 tgcgggcaac cacatcgcct gcgctggctg cggtgcgcac ttcgaaggac atctcctgag
     7621 gccatccaaa aggcgcttgc gtatagggag tgtagatggt ggccggagca tcgtctcgca
     7681 aacgatcgta tttggcgttc ttcgccacac cgatcacctc cacgtcccac tcctttttga
     7741 aggaccagcg aaacttcctg cccacgggcg atccggagcc gaaatgctgt agtgcgagcg
     7801 tctcgttgat cactgcaacc cggggcgtag cccccatgtc gcgcaactcg aacccgcggc
     7861 ccatgatcac aggaatgccc atcacttcga aaaactccgg tcccacgaaa ttgaatgtgg
     7921 atctcacggg tttttccgat gccgcgcctt ccaccaggac gcttccattt gacatgaaac
     7981 cgctgaccag gcgctgcgcg gagaggctgg ctcccgtgac gccgggaatc tgtttcaaac
     8041 gttccagcac gcgagcatag agttggtttc cacggttttc gtcatagccg ttttttcccg
     8101 gcgacaagtc gaagagcacg agattgtacg ggttgaaacc cagcgaaaga gaccgcaaat
     8161 tcgccaggct gcgagtgaaa agcgtggcgc cggccaacag cacgacggct acagctacct
     8221 gcaccgccac caggatgggt cccccgccaa accggcgcat tcgcacggcc ggcgtatctt
     8281 ccttgaggcc gctcgctacg tcgacacgcg tcgcccggat ggccggtgca agggcaaaga
     8341 gaaaggttgc cgccaacgaa accgcgacgg tgaaagcgag cacccgtgcg tccggcgaca
     8401 cggcgagcgg aatcggagtg gaccccgaag ccagcaaagc gagcaaggct cgcacacccc
     8461 aataggcaaa cgccactccc gcgatcgcgc ccgcagcgga gagcagggcc ccttccacaa
     8521 gcagttgacg caccaggcgc cagcggcttg ccccgaccgc caatcgcagc atgatctccc
     8581 gctgccgcgc cgtcgcgcgc gccagcaaca gcccggccag gttggcgcac gccatcagca
     8641 gcgtcagtgc gaccaccacc atcaggatca atagaggccg ccggtaaaca cgccgcaggc
     8701 tgtccagccc ctgcccgccg ggctcctgaa agatccgggg cgtctccgaa cccattatgc
     8761 tcctggcggt ctccggcagg ccggcgctca cgattgcggc caactcgttc ccggctgcgg
     8821 tctcttcttt ctcctggttc agacgcgcca tcacctggac ccagaacagg tcgtctccaa
     8881 accagttcac acgctggtgc ccggagcctt gcatccgctc cctggagcgg atgggcagcg
     8941 taatatccac tccgggagtt cgcataaacc caccggccga gaccccgaaa aattcccggg
     9001 gcgtcacgcc aatcacgatg caaggctgtc cattgacgta gagggtctta ccgattgccg
     9061 agggatcgag cgcgaacata cgttcccaga agcgatagct gatgaccgcg gaggtaagcc
     9121 cgtccgccgt atcgtcgtcc tccgtgatcg ggcgccccaa aacggtcccc acgccgagcg
     9181 cccggaaata attgcccgaa accaccatcg cgcccgccgc cagcgcctgg cgattgacgg
     9241 cgacgtttac gggacccagc ggcgaaaagc ccatgatgga gccgagtgtc tggcttcgca
     9301 cgcgtattcc ggaaaagatc tcccaggaaa aaacgttcgt cgtccagccg ccggagcctc
     9361 cgcggccgct ggcccaggtg ctactcagat tcaggttctt ctttgcgccc caccgcacga
     9421 ccacaagctg ctcgggattc tgcacgggca acatgcgcag cagaacggtg tcgagcaggc
     9481 tgaagacggc ggtgttggcg ccgattccga tggccagcga gagtaccgca atcgccgtga
     9541 acaccggcgc ctttcgcagg atgcgggcac cgtaacgaat gtcgcgtacc atgtcctcca
     9601 ggaacgtaag gccgcgaact tcgcggcact gttcccgaac ctgctctatg ccgccgaacc
     9661 gggcacgggc actccggcgc gcctcggtat cggccatccc cgcggcccgg tttttgcgcg
     9721 cctccatttc catgtggaag ctcagctctt cgtcgagttc gccttcggcg ctgcggtagc
     9781 gcaccaggga acgaagacgc agtatcagat cggtccagct catatcacgc catctccagc
     9841 acccggccga tggcgcccgc cattcgtgtc caggcctcgg tttccacttc caactgcttg
     9901 cgtccggcgg cggtcaggcg ataaaaccgc gcgcgccgcc ccagctccga gataccccat
     9961 tcggccttga tcgagccctg gtgctcaagc cggtgcagcg ccggatacag cgagccctgg
    10021 ttcacgcgga gaacgtccgc cgagacctgc tggatgcgct cagagatggc ccatccatgc
    10081 tgcgggcccg ccagcagcgt gcgcagaatc agcaggtcca gcgttccctg gagaagatcg
    10141 agtttcggtg tgcgattcat gttgtcgaat agctacaata agaatggcgc tattgttgtc
    10201 ggatgtcaac aacaaaaacg ccggccgtcc ggtttatttt ctggtgtcgt ttttcccggt
    10261 ttttggcgac ttgcccgaat cctcaaacga agaaacatgc acaccgatct tccgtcacaa
    10321 tctgatcgcc ggtgaacggt agaatccaaa ctcccccgaa gaattgcaga ccattcaaca
    10381 agccatcttc gatcagcttg tgacgcacga gctcaagaag cccaggctcc actaaccgtc
    10441 tcccgtttca cacccgccta ttccgcctga ttttcccgcg acgcctcacg gcacgcccat
    10501 gggtacaaca gcgggagcat atacagcgtc aacagcgtgg aagtaaccag accaccgatc
    10561 accacactgg ccagagggcg ctgcatctcc gagccgattg aagtcgacgt tgccatcgga
    10621 acaaaaccta aactggcaac gagggcggtc atcaacaccg gccggagacg agtgcctgct
    10681 ccttcgagaa tggcatcatg gaggggtttc ccgtccgctc gcagccggtt gatatagcta
    10741 accagaacga tgccattcaa cacggccact ccgaatagcg ccaggaaacc gattgacgcc
    10801 gacaggttca gattcaatcc gcgcagccaa agcgccccaa tgcctcccac cagggcaaac
    10861 gggacgttca ggatgataag aatcgcctgg ggcaccgaat cgaaggtagc aaacagcaag
    10921 gcgaaaataa tcagcacagt cagcggaagc acgatcgcga ggcgctgcat cgcccgctgt
    10981 tgattttcaa actggccgcc ccaatcggta ctatagccgg cgggtagcac aaggcccgtt
    11041 tccagcttac gttccgcctc ggccacgaag ctacccaggt cggtgccacg cacgttcgcc
    11101 tggaccacga tccgccgctg cccgtcttcc ctcgagacca cttccggccc gcggaccact
    11161 tccacgcggg ctacgctgcc cagcggcaca cgctcgccgg cgggcgcggc gatgagcagt
    11221 tgacgaagag actccgcatc cgcgcgatat cgctcaggga aacggatcac gatcggaaac
    11281 cgccgctggc cctcgatcat ctccgagaca tacttgccgg caacggctgc ttcgacgagt
    11341 tcccgcaccc tggtaatatc cagaccatag cgcgccaggg ccgtccggtc cacgtctacg
    11401 cgaagctccc ccacaccgga caggatctcc atctggagat ccgccgcgcc ggggacagtg
    11461 gccatcaatg tttgcgcccg ctccgccagt tgctcgagca cccgcggatc ctccccgaaa
    11521 atcttcaacg caacgtcggc ctttattccc gaaaccacct catccagccg catcgccatt
    11581 ggctgtgtga aactataggc aacaccgggg actttctcga gcgagtgcga cattttttcg
    11641 aataactctt cctttgtttc cgccgtcgtc cattctctga ccggcttcag gcggacgtag
    11701 acatcggcct cgtatattcc cattgcctcg gtggccatgt ccggccggcc cagctttgaa
    11761 accacacccg tcacctcggg gaacttcatg acaaccttct cgatttcgtc gctgagttcg
    11821 atcgattcgg tcagcgaaat accggggagt ttgcgagtct gaatgagcag cgagccttca
    11881 tccagcctcg gcataaactc tgtgccgatg tacgccagag aggcgagcga gacggtcact
    11941 aacgccaaac cggtgagaac ggcggccagg cggtggcgca ttgtgaagcg cagcgtagct
    12001 tcataggagc ggcgaacttt ggagaaccaa ccgccggtgt ggggtttcac gcccttacgc
    12061 agaacgatgg ccgccgcggt cgggacgacc gtcagcgaga agaccagcgc tcccaccagg
    12121 gccgagcaga cagtaatcgc catcggacgg aacatgcggc cttccaagcc ttccaggaac
    12181 ataatgggca tatagacggc aatgatgatc gccaccgcga atacgatggg ccgcgctacc
    12241 tcgtgagccg cgtgccggac ccgtccaagc acgttggcga tcttctcacc cggctttctt
    12301 tccagtttcc gaacggagtt ctccatcatc accaccgcgc catcgacgat catgccgaag
    12361 tcgagagcgc ccaggctcat cagattggcc gaaatgccga aaaccgccat gccgatgaaa
    12421 cctaccaaca gcgacaaggg aatcaccgag gctacgatga gcgccgcggg aatgttaccc
    12481 aggaataaaa gcagcaccgc cacgacaaga acgccggctt ccatcagatt tttccggacg
    12541 gtccctatcg ttccttcgat gacctccgac tgatcgtaga aaggcacgat ctttactccc
    12601 tcgggcagcc ggatggagga aagcttcgct ttgacgcggt cgattacccg gcggccgttc
    12661 tcgcccttca gcatgatcgc catgccggac accgtctcgc cctggccgtc gcgaagaacc
    12721 gctccctgcc gctgcatcgg agcgatggtg acgtgtgcga tatcccgaag cagcaccggc
    12781 gtacccgctc gcgccaggac gacgatgttg gacacgtccc caatactctg tgtgcggcca
    12841 agccccctga cggtgtactg ctccgcgtaa tgctcgatga agccgccgcc aaagttcgca
    12901 ttgttttcct ggacccggag gaatacgtca
//
LOCUS       DASTMK010000294         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_460679, whole genome shotgun sequence.
ACCESSION   DASTMK010000294 DASTMK010000000
VERSION     DASTMK010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460679"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VFQ79_12530"
     CDS             <1..1153
                     /locus_tag="VFQ79_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU0140539.1"
                     /translation="CEILGLLYRKRKLTRSEITQATGLNPASVSYALRYLLERGLLLK
                     TSGEVNGRRRELFDLNAEAGFFVAIDLEGDRIRFALTNLLGDVRFRWEEPLPFRQPLE
                     PRKLIEGIGRVVRDKDTKLPPVLAIGVSYPGLLDSDGRLTAVNLGWDRFPLVQILNEA
                     FPWPLFLESDKHSCIQAERWLGSAQNCDNALFLIAEGGVGLGILTDGKPLAGAYFLPG
                     EIGHLKILPDADDLCNCGERGCLEAIASSPNIVRQYLERGGLSRPGGAPLRVTDVFEE
                     ARQRDPAALAVIERAGCVLGLSLSYAITLLNPEIVILGGDLCAAEDLFLPIIKREIER
                     RTISLSLTGVNIRVSSLGSDIRLKGAASQAFGKMLRDPGLLSKLCTSAV"
     gene            complement(1236..2165)
                     /locus_tag="VFQ79_12535"
     CDS             complement(1236..2165)
                     /locus_tag="VFQ79_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0140540.1"
                     /translation="MLTRRGFLSTTGVAAAQKVSPVIADKDDISLAAWSLNRSFFLAK
                     RWKNIDLPRIAREEFNINGLEFVNTFFENPTMHYLQDLKKRGEKHGVAFVLIMVDGEG
                     DMSAKDKQERTQAAIAHRKWVDIASFLGCKAIRCNMGGPRENWQQDADLVGRAAESFY
                     NLVEYASTAELNILIENHGGASSDPDVVVSLMKTVDHPRFGTLPDFGNVNPGADHAEV
                     IRKLAPFAKGVSVKAAWTAEGTHPGWDLEKLIGICRDAGYKGFWGIESSFGRYGRPRG
                     NEETPPPPARIWQNEARGVQLTKAVLQKALRIA"
     gene            complement(2179..3051)
                     /locus_tag="VFQ79_12540"
     CDS             complement(2179..3051)
                     /locus_tag="VFQ79_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0140541.1"
                     /translation="MYRICRFLLLLSAAVPLLAQADGKLRIIAFGAHPDDCDSRAGGT
                     AAKYAKLGHHVKFVSVTNGDAGHQSEGGGALAKKRREEAREAGRRIGVEYETLDNHDG
                     ELLPTLAVRHQVIRKIRQWNADVVLAPRPNDYHPDHRYTGVLVQDAAYMVVVPNVCPD
                     TPPLRKNPLFLYFQDGFQRPNPFRPDVAVAIDDVIDLKVAMLDAHTSQMYEWLPWVAG
                     NLDSVPRDPKARVEWLRKTRFSRPVTDAVRATLKKWYGSRSDSVRHAEAFEVCEYGRR
                     PTDEDLRKVFPFFE"
BASE COUNT          649 a          915 c          947 g          687 t
ORIGIN      
        1 atgtgaaatc ctgggcttgc tgtatcggaa acggaagctg acccgcagcg agatcaccca
       61 ggctaccggg ctcaaccccg ccagcgtaag ttatgcgctg cggtacctgc tcgagcgcgg
      121 cctgctgctg aaaacaagtg gcgaagtcaa cggtcggagg cgcgagctat tcgacctgaa
      181 cgcggaggct ggttttttcg tggcgatcga tttagaggga gatcgcattc gcttcgcgct
      241 aacgaatctc ctcggcgatg tccgctttcg gtgggaggaa cccttgccgt ttcggcagcc
      301 gctcgagcca cgaaaattga tcgaaggaat cggtagggtc gtgcgggaca aggatacgaa
      361 gctcccgccg gtgctggcga ttggcgtgag ctatccaggg ctgttagatt cagacggccg
      421 gctgacagcg gtcaacctgg gatgggacag gttcccactc gttcagatat tgaacgaagc
      481 cttcccttgg cccctgtttc tggaatccga caagcatagt tgtattcaag cggagcgatg
      541 gctgggatcc gcgcagaact gtgacaacgc gctattcctg atcgcagagg gaggagtcgg
      601 tttgggcatc ctgacggatg gtaaacccct cgccggagcg tactttcttc caggagagat
      661 cgggcattta aagattttgc ccgacgcaga tgatctctgc aattgcggcg agaggggctg
      721 tttggaggcg atcgcctcca gtcctaatat cgttcgtcag tacctggagc gaggcgggct
      781 atcgcgaccg ggaggcgccc cacttcgggt aaccgacgtt ttcgaagaag cgcgacagcg
      841 ggatccggca gcactagcgg tcattgagcg ggcaggctgt gtccttggtc tttcgctttc
      901 gtatgcgatc acgctgctga atccggagat cgttatactg ggcggcgatc tttgtgccgc
      961 tgaggatctc ttcttgccga tcattaagcg ggagatagag cggcggacaa tatcgctctc
     1021 cctcaccggc gtcaacatca gggtcagtag tttgggcagc gacatccgcc tcaaaggagc
     1081 tgcatcgcag gcgtttggaa aaatgcttag agatccgggt ttgctgagca agctttgcac
     1141 ctcagccgtg taaacatctg gcgaggaacc tgttactgtg cggctaactg ggtggtgctc
     1201 agcaaaacca ccggacccgc cggccagcgg cgaaatcagg cgatgcgcag cgctttttga
     1261 agaaccgctt tcgtgagttg aacgcctctc gcttcgttct gccagatcct ggccggagga
     1321 ggcggcgtct cttcgttgcc gcgcggacgg ccataccgcc cgaagctcga ttcgatgccc
     1381 caaaaacctt tgtagcccgc atcccggcag atgccgatca gtttttcgag atcccatccc
     1441 ggatgggttc cctccgctgt ccaggcagcc ttcaccgaaa cgcctttcgc aaacggagcc
     1501 agtttccgga tgacttccgc atggtcggca ccgggattca cgttcccgaa atccggcaac
     1561 gttccgaacc tgggatgatc gacggttttc atcagggaaa cgacgacatc gggatcggac
     1621 gaagcgccgc cgtggttctc gatgagaata ttcaactcgg cggtggaggc atactcaacc
     1681 aggttgtaaa agctttcggc ggcgcgtccg accaggtccg cgtcctgctg ccagttctcg
     1741 cgcgggccgc ccatattgca gcgaatcgcc ttgcagccga ggaagcttgc aatatccacc
     1801 cacttgcggt gcgcgatggc agcctgcgtt cgctcctgct tgtctttggc ggacatgtcg
     1861 ccttcgccgt ccaccatgat caggacaaaa gcgacgccgt gtttctcgcc gcgctttttc
     1921 aggtcctgaa ggtagtgcat ggtggggttc tcaaaaaagg tattgacgaa ctccaaccca
     1981 ttgatgttga attcttcccg ggcaatgcgc ggcagatcga tgttcttcca tcgtttcgcc
     2041 aggaagaaac tgcggttgag cgaccaggcg gccagagaga tgtcatcttt gtccgcgata
     2101 acgggactta ccttctgagc ggcagcaacc ccggtggtac tgagaaatcc tcttctcgtc
     2161 agcatgcgaa tgagtccgtt attcgaagaa cgggaacacc ttcctaagat cttcgtcggt
     2221 gggccgccgc ccgtactcac agacttcgaa tgcctcagcg tgcctgacgg aatccgaacg
     2281 agagccgtac cattttttca gcgttgctct caccgcatcc gttaccggac ggctgaaacg
     2341 ggtcttccgg agccattcca cacgcgcttt tggatctcgc ggaacagaat ccagattgcc
     2401 ggccacccac ggcagccact cgtacatctg cgaggtgtgc gcgtccagca tggcgacttt
     2461 cagatcgata acgtcatcga tggcgacagc cacatccggg cgaaacggat taggtctctg
     2521 aaaaccgtcc tggaaataaa ggaagagcgg gttcttccgt aaaggcggtg tatcgggaca
     2581 gacgttcggc accactacca tgtaagccgc atcctgcacc agcacgcctg tatagcggtg
     2641 gtcggggtga taatcgttcg gccgcggagc caggacaacg tcggcgttcc actggcggat
     2701 tttccggatc acctggtggc gcacggcaag ggtgggaagt agttcgccgt catgattgtc
     2761 gagcgtctcg tactcgactc cgatccggcg gccggcttca cgcgcctctt cgcgccgttt
     2821 tttggccagg gccccgcctc cctcggactg atgcccggca tcgccgttcg taactgacac
     2881 gaacttgacg tgatggccga gctttgcgta cttggcggcg gtcccgcccg cccggctgtc
     2941 acagtcgtcc ggatgcgcgc caaaggctat gatgcgcagt tttccgtcgg cttgcgccag
     3001 cagcggcacg gcggcggaca gcagaagaag aaatcgacaa attctataca taaacggaaa
     3061 tccctatcag aaaacatcat atacgcagtt gcgcccggtt gcgtgaggaa ggtgagccta
     3121 tgctttcagt aactcgcgca cgaactgcgc cgtctggccg atctgatcgt tgaaacgctg
     3181 atacatgccg atgatcag
//
LOCUS       DASTMK010000295        13185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_15941, whole genome shotgun sequence.
ACCESSION   DASTMK010000295 DASTMK010000000
VERSION     DASTMK010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15941"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFQ79_12545"
     CDS             complement(<1..862)
                     /locus_tag="VFQ79_12545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0140542.1"
                     /translation="MQYPLRGFWITVAILWAVLCIAGYVYSKQQGIPDSAALAVILAC
                     LVEAAFYIAPGFASVRQRLSGVRSRYLLAGFLAVSALVPYVIFAGLSSTFEWPSFALL
                     GLIAAVIPFWFAILPKHPATDLGFLALVAIVFLSTIFQSIYVDPHPRVPAEMFGKLMS
                     IRLGIASALFLRGVKGTGFGFVPERKDWKIGILHYLYFLPVGAALIALTGFATLRSLP
                     VDPAKLALTAFASFLGFLWVVALSEEFFVRGLLQQWLSKWMSSPVAGLLIASLIFGLL
                     HLPFRSAFPNW"
     gene            870..1607
                     /locus_tag="VFQ79_12550"
     CDS             870..1607
                     /locus_tag="VFQ79_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140543.1"
                     /translation="MVAFVVFVPSVFTAEPPPGLPKKVAVRETENEAARADYTYRQSV
                     VIEEFDDRGMITGRYREIRDIIFSPKGRTEQMVESPTNSLKRLLLTPEDFRDIREVQP
                     LLLTSDQLWIYETRFRGEEQVNGIDCWLLEVRPRQILSGQRLFEGMLWVDQRDYSIIQ
                     TQGKAVPEILSLKSENRFPRFTTLREQVDGKYWFPQQTVADDTLHFRSGPQRIRMIIR
                     YSDYKKFSAESTVTFEDAPANPPQSQR"
     gene            1650..2207
                     /locus_tag="VFQ79_12555"
     CDS             1650..2207
                     /locus_tag="VFQ79_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140544.1"
                     /translation="MRAFVAALVLAGLLAVQASTSDYVSAKSKIQAIEKQRAPARVTL
                     NEAELNAWIRGELPDLWKEGLRNPRVELNNGTASGYALVNFARLKQAGGDPPGWFTRA
                     LLDGEKPVSVTTRVRTHDGMAQVDVENVTVSGIPIEGAALDFLIRNFVRSRYPEAKIG
                     QPFEMGYGMQKLELKPKHVTILMRK"
     gene            complement(2185..3564)
                     /gene="hemG"
                     /locus_tag="VFQ79_12560"
     CDS             complement(2185..3564)
                     /gene="hemG"
                     /locus_tag="VFQ79_12560"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEU0140545.1"
                     /translation="MRRAVIIGGGISGLSTAYYLAKAGIEPTLIEARSHLGGVIRTDT
                     LEGCVIEAGPDSFLSAKPWAMDLICELGLADEVIGSNDHLRVTYVVKHGRLVPLPDGL
                     MMMVPTKVMPIIASGLLSWPTKLRMGLEFFRRPPAERPRDRSVSEFIEDHYGREAVEY
                     LAEPLLAGVYGGDPNRLSVSSVLPRFAELEYEYGSLTRGVLDQRRKRKRSDASGPLFR
                     TLKRGLGHLIETLTGAIGSRMEVIQGHADAMEKTPAGFRIRVNGAWLEASHVVLACQA
                     YAAAPLAGMLDPELGRCLESIPYSSSMTLALGYRRQSFGQSLKGFGFLVPRKERRTLV
                     ACTWVGNKFAHRIPEEWVVLRCFVGGLDDSFLRESDETITAAVRAELSGIMGVTAAPE
                     LVRISRWPRSMAQYTVGHESRLKEIEQRTARIEGLSLAGNAYYGIGVPDCIRLGKQAA
                     GRITSASES"
     gene            complement(3564..4445)
                     /gene="hemH"
                     /locus_tag="VFQ79_12565"
     CDS             complement(3564..4445)
                     /gene="hemH"
                     /locus_tag="VFQ79_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU0140546.1"
                     /translation="MKQAVLLLAHGAPETLDDVEKYLEYIRPGRPLPAPVIREIKRRY
                     EEIGGGSPLLARTREQAEALQAVLAPVRVYFGMRNWHPFIAETMEQMQRDSIERIVAI
                     CLAPQYSRVSVGLYFRRTQEAKRALHMTSEIVWAKSFHDHPMLIDAFAAKLLPLLPAE
                     KVLFTAHSIPEEKVVQSPDPYSKEAKTTASLVAERAGVEDWDFAYQSEGMTGENWLGP
                     PVESRLDAFAAEGVRNVVIHPIGFVSDHVEILYDVDILFRRYARERGIGITRPESLNS
                     SPELISALAGVASQKLV"
     gene            complement(4442..5479)
                     /gene="hemE"
                     /locus_tag="VFQ79_12570"
     CDS             complement(4442..5479)
                     /gene="hemE"
                     /locus_tag="VFQ79_12570"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU0140547.1"
                     /translation="MRVAPMNSRFLDACRRRPTDVRPVWFMRQAGRYLKQYREVRSKH
                     SILEICKRPDLAATVTLQPIEILDVDAAIIFADLLLPVEPMGLKLEFVAGEGPRIDNP
                     VRTSGDIDTLSISNTDDLGYVGESIQIVVKALGGRAPVIGFVGAPFTLASYMIEGGPS
                     RTFLKTKQLMYNDETLWRRLMGKIVDVLGPYALIQVSAGARAIQVFDSWAGALGPDDY
                     VRYVQQYSRALIERIRSSGVPVIHFGTGAAGFFRELHAAGGDVMGVDWRINIDQAWMD
                     ISYRSAIQGNLDPAVLFAPLSELRVRVHELLKRTGTKPGHIFNLGHGVLPETPVENVK
                     AVVQLVRELRP"
     gene            complement(5461..6426)
                     /locus_tag="VFQ79_12575"
     CDS             complement(5461..6426)
                     /locus_tag="VFQ79_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU0140548.1"
                     /translation="MKQFVLLLLLAPALGLSGDLLRLEDTQEAMGTAFSVVAYGQDAV
                     RMRAAVEQSFEEVRRLDHLLSNYRPNSEWSQVNRDAAGRPVRVSQELFDLIQACVDYS
                     RRSEGTFDITVGPLMKLWGFYKGSGRLPHRAEVRAVLSKVGYGNIVLDPAERTVRFTR
                     KGVEIDPGGIGKGYAVDRMVEVLRSGGIDSALITAGGSSIYAIGGPRGEKGWRVAIRD
                     PRKHDRTIEEVCLRDESMSTSGSYEKFFRAEGRMYSHIMDPRTGFPAEGTLSVSVVAP
                     RTIDSEAWTKPFFIQGREWTAQHKPKGFRVFLCEDKVGQACAWLQ"
     gene            complement(6426..6818)
                     /gene="rbfA"
                     /locus_tag="VFQ79_12580"
     CDS             complement(6426..6818)
                     /gene="rbfA"
                     /locus_tag="VFQ79_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU0140549.1"
                     /translation="MEERRLQRVSEAMREELAEIVGFELTDPRIGAVSVTDVHVSPDS
                     RHARVRVALSGKPEERKESLEALEGARHYVRRELARRLELFRIPEIHFEADALVDPSR
                     LDVLIKRIRKGRPRSPAPDEGSSKKNPF"
     gene            complement(7018..7305)
                     /locus_tag="VFQ79_12585"
     CDS             complement(7018..7305)
                     /locus_tag="VFQ79_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEU0140550.1"
                     /translation="MASIGVLTLELRMEHAHSLKEKRSVVKGLKERLRQRFNVSVAEI
                     DYQDTWQRAVVAAVTVASDHDRASSVLQSVEKEAVALLGPDLVGATLEWLE"
     gene            complement(7319..10288)
                     /gene="infB"
                     /locus_tag="VFQ79_12590"
     CDS             complement(7319..10288)
                     /gene="infB"
                     /locus_tag="VFQ79_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU0140551.1"
                     /translation="MKKIRINELARELEVKPHRIIELLPELGVSEKKTHSSSIDEDVA
                     EIVRQRLGGLGDHPGVLDALSASNAVVEVEEPELTGKDHEAVPRPEASGPEEQAEEPG
                     PETAAEEPVAPAPVQQASRPRPIRPPLAGAGARPEISAEEGQPAAPPPAVETPLGAPR
                     STATEPPPPVPRQAPSIPVAPVGQKAGAIPAKPLPAPRPGQILTGPRQPFPGEAGPVP
                     GMPRPSAPPRPPVSRPAAPPSGQPWPPQARPRPAATPQTPPLSRPPLAGQPAARPVVP
                     PDPKLIERIRAQQQTQPKAMPGQPQARPPMPGRPQTTPVPGQPIYRGPIRPGQALVQR
                     PGGPGARPGGGRRPMHPTSPMGIPPSPLPPDQQRRDTGKRARVVQRDRESELEGKMFQ
                     PRRHKQEGPPPIDREITISEGITVKELSEKLGVKANLVIKKLVEKKVFATINQALDPK
                     LAEDVAREFGASINKVSYEEEATQGLVQSEDEKDLTRRAPVVTIMGHVDHGKTSLLDA
                     IRATNVAEREAGGITQHIGAYQIVKNDRKIVFIDTPGHEAFTRMRARGAKVTDIVVLV
                     VAADDGVMPQTLEAIDHARAAEVPIVVAINKIDKPDAQPERIKQQLSDRGLLPEDWGG
                     ETVMVPVSAKSQQNLDLLLEMILLVADMEDLKANPQRPAVGTVLEAQLDRGRGPVATV
                     LVQNGTLHVGDFFICGAVFGKVRAMFSDVGQQLKDAEPSTPVEVLGLDSVPDVGDTFQ
                     VVTDTAKAKQIVLYRESKAREQSMLKAPKLTLDQLHEQLREGETKELPIILKADVGGT
                     AEVVADTLQKLSNEKVKVRVLHSGVGAINESDVLLASASNAVIVGFNVRAERNATSLA
                     EQEKVDVRLHTIIYELTDEIKRAMTGMLEPVFKEVYRGRAEVREVFRITKVGTVAGCM
                     VLDGTITRDSQVRLLRDNIVVHTGKLESLRRFKNDVSEVKSGFECGIALANFGDLKPG
                     DIIEAFVTERVAVQVSA"
     gene            complement(10347..11891)
                     /gene="nusA"
                     /locus_tag="VFQ79_12595"
     CDS             complement(10347..11891)
                     /gene="nusA"
                     /locus_tag="VFQ79_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU0140552.1"
                     /translation="MASIYQSIEILSKEKGIEPHIVLDAVKDAMLVAARKHFRTEEDL
                     VAELNERTGAIQIYAVRKVVETVEDPVREIALSEALDIDPNAEQGSEIRIPKSMDALG
                     RISAQTAKQVILQKVREAERENIYAEYSGRVGELVNCTVKRIEGQDLIMDLGKTEARL
                     PKKEQSRLESFSVGERVRCVMRSVEKTGKNAGVIVSRAAPEFVMRLFEQEVPEIYDNT
                     VVIKGCAREAGERTKIAVFSRDRDVDSVGACVGMKGMRVQSIIRELRGEKIDIIEYSE
                     DPVVFATHALSPAKISRVSIVNPSERHMEVIVDDSQLSLAIGKKGQNVRLAAKLLGWK
                     VDIKSEEEKRQEVESQMAALGVSGAPVSVLIDHGLGESIVEKLVEGGVGTVERLGAMT
                     PEQLEEIAGIGPKLIEKIQAAVVAYYGQFEEPAFEGEEEAAQVDAALQSEVEMAPDEA
                     VDSMEPAAANEEESASEVADAGIAEEASEAPPAPATESESGEGQTESSPGDGQEVEFD
                     TIRDSD"
     gene            complement(11923..12387)
                     /gene="rimP"
                     /locus_tag="VFQ79_12600"
     CDS             complement(11923..12387)
                     /gene="rimP"
                     /locus_tag="VFQ79_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041858220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU0140553.1"
                     /translation="MKDSVVAKVTEIAERVAGSEGLEVIDVELRGGGKARLLRIVIDK
                     PEGVSHGDCEFISKQVGTILDVEDVIPGGSYHLEVSSPGLERPLKKPKDFERFLGRKI
                     AVSLRTPIEERRRWDGVLAGFSEGLVTLEPASGKSVQFTLDNIEKANLKFEW"
     gene            complement(12537..12884)
                     /locus_tag="VFQ79_12605"
     CDS             complement(12537..12884)
                     /locus_tag="VFQ79_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140554.1"
                     /translation="MATFNFFTASKGFEDESVWLTQAAIADLFQTTPQNITLHIGAIY
                     EEGELGEEATCKEYLQVRQEGSRQVTRRLKHYSLNVVLAVAKPRPVDADLEKAVEQAK
                     QINAARPKRKPKE"
BASE COUNT         2528 a         4256 c         3711 g         2690 t
ORIGIN      
        1 gccagttggg aaaggccgat cgaaacggca ggtgcagcaa cccgaaaatc agggaagcga
       61 tcagcaggcc cgccaccgga ctgctcatcc acttcgacaa ccactgttgc aggagtccgc
      121 gtacgaagaa ctcctcggaa agcgcgacca cccacaggaa cccaaggaag ctggcgaatg
      181 ccgttagcgc cagctttgcc gggtccaccg gcagcgagcg cagcgtcgcg aagccggtga
      241 gagcaatcag ggccgctccc acgggcagga aatagaggta atgcagaatg ccgatcttcc
      301 agtcttttcg ttccggcaca aagccgaacc ctgtcccttt cactcccctg agaaagagcg
      361 cggaagcgat acccagccgg atggacatga gctttccaaa catttccgcc ggtacgcgtg
      421 gatgagggtc gacgtagatg gattgaaaaa tagtcgaaag gaatacgatc gcgacgagcg
      481 cgagaaagcc gaggtcggtg gccggatgct tcggcagtat ggcgaaccag aacgggatga
      541 cagcggcgat caggcccagc aacgcgaagg acggccactc gaaggtagag gatagccccg
      601 cgaaaataac gtagggaacc agtgcgctca ccgccaggaa gccggcaagc aggtaccggc
      661 tccgtacgcc gctcagccgt tgccggacgg aagcaaagcc gggcgcgatg tagaaagccg
      721 cttccaccag acaggccagg atcacagcaa gggcggctga atccgggata ccctgttgtt
      781 ttgagtagac gtaccctgca atgcacagaa cggcccacag gatcgcaact gtgatccaga
      841 aacctcggag cggatactgc attcgcctca tggtagcatt cgttgttttt gttccctccg
      901 ttttcaccgc cgagccgccg ccgggattgc ccaaaaaggt tgccgtacgg gagactgaga
      961 acgaggctgc gcgagccgat tacacttacc ggcaaagcgt ggtcatcgag gagttcgacg
     1021 atcgcgggat gatcacgggc cggtaccgcg agatccgcga catcattttc tcgcccaaag
     1081 gccgcacgga gcagatggtg gaatcgccca ccaacagcct caagcgcctg ttgcttacgc
     1141 ccgaggattt tcgggacatc cgcgaagtgc agccgctgct gttgacctcg gaccagctat
     1201 ggatttacga aacacggttc cgcggcgagg agcaggtaaa cgggatcgat tgctggctcc
     1261 tggaggtccg cccgcgccag attctctccg ggcagcgcct gttcgagggc atgttgtggg
     1321 tcgaccaacg cgattactcc atcatccaga cgcagggcaa ggcggtaccg gaaattctgt
     1381 cgctgaaatc ggaaaaccgc ttcccccggt ttaccaccct ccgcgaacag gtggatggca
     1441 aatactggtt tccccaacag acggtggcgg acgatacgct tcacttccgg agcggtccgc
     1501 aacgcatccg gatgatcatc cgttacagcg actacaagaa gttctcagcg gaatccacgg
     1561 tgacgtttga ggacgcgccg gcaaatccac cgcaatcaca acgctgacac ggcctgcgcg
     1621 caattctcaa attaccggat aatacaaata tgagggcttt cgttgcggcc ctggtcttgg
     1681 caggcttact ggcagtccag gcttccacct ccgactacgt ttccgcgaag agcaagatac
     1741 aggcgattga aaagcagcgg gcgcccgcgc gtgtcacttt aaacgaagcc gaactgaacg
     1801 cctggatccg gggagagttg ccggatttat ggaaggaggg actgcggaat ccgcgggtcg
     1861 agttgaacaa cggcacagcc tccggctacg ctctcgtgaa tttcgccagg ctgaagcagg
     1921 cgggcggcga tccacctgga tggttcacgc gcgctctact cgacggtgaa aaaccggtga
     1981 gcgtcacgac gagagtacga acccacgacg gcatggccca ggtggatgtg gagaacgtca
     2041 ccgtttccgg cattcccatc gaaggcgccg cgttggattt cctgatccga aatttcgtgc
     2101 ggtcgcggta tcccgaagcc aagatcggac agcccttcga gatgggctac ggaatgcaaa
     2161 agctcgaact caagcccaaa cacgttacga ttctgatgcg gaagtgatcc gtccggccgc
     2221 ctgtttgccc aaccggatac aatcggggac accgattccg taataggcat tgccggcaag
     2281 cgagagccct tcgatgcgtg ccgtccgctg ctcgatctcc ttgaggcggc tttcgtgacc
     2341 gacggtatat tgtgccatcg atcgcggcca gcgcgagatg cgcacaagct ccggcgcggc
     2401 ggtcactccc ataatgccgc tcagctcagc ccgtacggcg gcggtgatgg tctcatcgct
     2461 ctctcgtaga aagctgtcgt ccagaccacc tacaaaacag cgcagcacca cccactcctc
     2521 cggaatccga tgggcgaatt tgtttcccac ccacgtacag gccaccagcg ttcgccgttc
     2581 ctttctgggg accagaaatc caaacccctt cagcgactgt ccgaacgatt gccgccggta
     2641 tcccagggcg agcgtcatcg atgaactata gggaatcgac tcgaggcaac gcccaagctc
     2701 gggatccagc atgcctgcca atggggcagc cgcatacgcc tggcatgcca gcactacgtg
     2761 cgacgcttcg agccacgcgc cgttgacacg aattcgaaat cccgccggag tcttctccat
     2821 cgcatcggcg tgtccctgta tcacttccat gcggctcccg atcgctccgg taagcgtttc
     2881 aatcaggtgc cccagccctc gcttgagtgt ccggaacagc gggccggaag cgtcgctccg
     2941 cttccgcttc ctgcgctgat cgagcacgcc tcgcgtgagg ctgccgtatt cgtattccag
     3001 ttccgcgaag cgcggcaaca cgctcgagac gctcagccgg tttggatcgc cgccatagac
     3061 gccggcaagg aggggctcag ccagatactc gaccgcttcc cgcccgtagt gatcttcgat
     3121 gaactcggac accgaccggt ccctagggcg ctcggcgggc ggccggcgga agaactcgag
     3181 gcccatccgc aatttcgtcg gccagctcag aagtcccgaa gcgatgatgg gcatcacctt
     3241 ggtcggcacc atcatcatca agccatcggg cagcggcacc agccttccgt gctttaccac
     3301 gtaggtcacg cgcagatggt cgttcgagcc aatcacctca tccgcaagcc cgagttcgca
     3361 aatcaggtcc atcgcccacg gcttggcgga aagaaagctg tcgggaccgg cttcaatcac
     3421 gcagccttca agcgtgtcgg tgcgaatcac tccacccagg tgcgatctgg cttcgatcaa
     3481 agtaggttcg atgccggcct tggcaagata gtaggccgtg gaaagacccg agattccgcc
     3541 gccgatgatg accgcccgcc gcatcacacc aacttctgcg aagccactcc ggcaagagcc
     3601 gagatcaact ccggggaact gttcagcgac tcgggacgag tgattccgat tccccgttcg
     3661 cgcgcgtacc ggcggaacag gatatcgaca tcgtacagga tctcgacgtg atcggaaaca
     3721 aatccgatcg ggtggatcac cacgttccgg accccttcgg ctgcaaacgc atccaggcgg
     3781 ctttccaccg gcggccccaa ccagttttca ccggtcatcc cctcgctctg atacgcgaaa
     3841 tcccagtctt caacgcccgc ccgttccgcc accagagatg ccgtggtctt cgcttctttg
     3901 ctgtaaggat cggggctttg aacaactttt tcctccggga tgctgtgcgc cgtgaagagc
     3961 actttttccg ccggcaggag cggcaagagt ttcgcggcaa aggcatcaat gagcatcgga
     4021 tggtcgtgaa agcttttggc ccagacgatt tcggaagtca tgtggagcgc ccgcttggct
     4081 tcctgggtac ggcggaagta aagacctacg ctgacgcgcg aatactgcgg cgccaggcag
     4141 atggcgacga ttcgctcgat cgagtcgcgc tgcatctgct ccatggtctc ggcgataaac
     4201 gggtgccagt tacgcatccc gaaatagacc cttacgggag cgagcaccgc ctggagcgct
     4261 tccgcctgct cgcgcgtacg ggccagaagg ggcgacccgc cgccgatctc ctcgtaccgc
     4321 cgtttaattt cccggatcac cggcgccggc agtggacgtc ccggacgtat atattcgaga
     4381 tatttctcca catcgtccag cgtctcgggc gcgccatgcg ccagcagcag gacggcctgc
     4441 ttcacggacg cagttcccgc accagttgca ccacggcttt cacgttctcg acaggagttt
     4501 ccggcaacac gccatggccc aggttgaaga tgtggccggg cttcgtgccc gtacgcttca
     4561 gcagttcatg cacgcgcacg cgcagttcgg ataaaggcgc aaacagcacc gccggatcga
     4621 ggttgccttg aatcgcggac cggtagctga tgtccatcca cgcctggtcg atgttgatgc
     4681 gccagtcgac gcccatgaca tcgccgccgg cggcgtgcag ttcccggaag aatcccgccg
     4741 cgccggtgcc gaaatgaatg acgggaactc cactggaacg gatgcgttca atcagcgccc
     4801 gcgagtactg ttgaacatag cggacgtagt cgtccggccc aagcgctccg gcccagctat
     4861 cgaatacctg gatcgcccgg gcgccggccg aaacctgaat aagcgcatac ggtcccagca
     4921 catcgacgat cttgcccatc agccgccgcc acagggtctc gtcgttgtac atgagttgct
     4981 tggtcttgag aaacgtccgg gaaggcccgc cctcgatcat gtagctggcc agcgtaaacg
     5041 gcgcgccgac aaacccgatt acgggagctc ttccgcccag cgccttcacc acgatttgaa
     5101 tcgactcgcc aacgtaaccg agatcgtcgg tgttggagat ggacaaggta tcgatatcgc
     5161 cggacgtccg cacgggattg tcgatacggg gtccttcacc cgccacaaac tcgagcttca
     5221 gccccatcgg ttccaccggc agcaggaggt cggcgaaaat aatggctgcg tcgacatcca
     5281 ggatttcgat cggttggagc gttaccgtgg cggccagatc cggccgcttg cagatctcga
     5341 ggatgctatg tttcgaacgc acctcgcggt attgcttcag gtaccggccc gcctggcgca
     5401 tgaaccatac gggtcttaca tccgtaggtc tgcggcggca ggcgtcgagg aaacggctgt
     5461 tcattggagc cacgcgcatg cctgtcccac tttgtcttcg cagagaaata cgcgaaatcc
     5521 tttcggttta tgttgggccg tccattcccg gccctgaata aaaaacggct tggtccacgc
     5581 ttcgctgtcg atcgtccgtg gagccactac ggaaaccgac aacgtccctt ccgccggaaa
     5641 gcccgtgcgg ggatccatga tgtgactgta catccggccc tcggcccgga agaatttttc
     5701 ataacttccc gacgtggaca tggattcgtc tctcagacac acctcttcta ttgtgcggtc
     5761 gtgtttacgc ggatcccgaa tggccacccg ccatcccttt tcaccgcgag gtccgccgat
     5821 cgcgtagata ctgctgcccc ccgccgtgat cagcgccgag tcgataccgc ccgatctcaa
     5881 aacctctacc atccggtcta cggcataccc ttttccgatg ccgcccgggt cgatctctac
     5941 tcccttccgg gtgaaacgaa cagtgcgttc cgccggatcg agcacgatat tgccgtatcc
     6001 caccttcgag agtacggccc gcacctccgc gcgatgcggc agccggccgg agcctttgta
     6061 aaaaccccac aatttcatca gcgggcccac tgtgatatcg aacgtccctt cgctgcggcg
     6121 gctgtagtcc acgcatgcct ggatgagatc gaacaattcc tgcgataccc ggaccggccg
     6181 cccggccgca tcccggttta cctggctcca ctcgctgttc ggacggtagt tcgacagcag
     6241 gtggtccagg cgccggactt cctcgaaaga ctgctccacc gcggcccgca tccggactgc
     6301 atcctgcccg taggccacca ccgaaaacgc cgtgcccatg gcctcctggg tatcttccag
     6361 gcggagaaga tctccggaaa ggcccagtgc cggggccaac aacaaaagta gcacaaactg
     6421 tttcatcaga aagggttttt cttcgaagaa ccttcatccg gcgccgggga ccgggggcgg
     6481 cccttgcgaa tacgcttgat gagaacatcc aggcgggaag ggtctaccaa cgcatcggct
     6541 tcaaaatgaa tctcggggat ccggaaaagc tccaggcgcc gcgccagttc ccgccggacg
     6601 taatgccggg cgccttcgag cgcctccagg ctctccttgc gctcttcggg cttgcctgac
     6661 agcgccaccc tcacacgcgc gtgccgggaa tccggggaga cgtggacgtc ggtcaccgaa
     6721 accgcgccaa tccgggggtc ggtcaattca aagccgacga tctccgccaa ttcttcccgc
     6781 atcgcttcgg aaacacgctg aagacggcgc tcttccatcg tcggactcct ctacttcttg
     6841 cctccaccgc cgctccgtaa gaaggcgtga aagattcaga gccacagatg gacacagatg
     6901 aacacagatt gaaaacaggg aacttatctg tgttcatccg tgttcatccg tggctaaatt
     6961 caatttcttc acagcttctc agcaagcggt aagtcaagcc acggttcttc tcgtttgtta
     7021 ctccagccac tccagggtgg ccccaaccaa atccggcccc aacagggcta cagcttcttt
     7081 ttcgacggac tgcaataccg aactggcgcg gtcgtgatcg cttgctaccg taacagcggc
     7141 caccaccgcc cgctgccacg tatcctgata atcgatttcg gccaccgaga cgttgaagcg
     7201 ctgccgcagc cgctctttca agcccttcac gacgctccgt ttttctttaa gcgagtgggc
     7261 gtgctccatc cgcaattcca acgtgagcac gccgatgctt gccatcgaga acttgcgcct
     7321 atgccgaaac ctgcaccgcg actctctcgg tgacgaacgc ttcaatgatg tcgcccggct
     7381 tcaggtcgcc gaagttggcg agcgcgatac cgcattcaaa acccgatttc acttcgctga
     7441 catcgttctt gaaccgccgc aaagactcca gctttccggt gtggacgaca atattgtcgc
     7501 gcagcagccg cacctggctg tcgcgggtaa tcgttccatc caacaccatg cagcccgcga
     7561 ccgtgcccac cttggtgatg cggaaaacct cgcgcacctc ggcccggccc cggtagactt
     7621 ccttgaagac gggctcgagc ataccggtca tcgcgcgttt tatctcgtcc gtcagttcgt
     7681 agatgatcgt gtgcaggcgg acatccacct tttcctgttc ggccagggaa gtggcattcc
     7741 gttccgcccg gacgttgaag cccacgatga cggcgttgga tgccgacgcc agcagcacgt
     7801 cgctctcgtt gatggcgccg acgccgctgt gcaaaacgcg cacctttacc ttctcgttgg
     7861 acagcttctg cagggtgtcc gctaccactt ccgccgttcc gcccacatcg gccttgagaa
     7921 tgatcggcag ctctttcgtc tcgccctctc tgagctgctc gtgcagttga tcgagtgtga
     7981 gcttgggcgc tttcagcatc gactgctcgc gggccttcga ctcgcggtaa agaacaatct
     8041 gtttggcttt agcggtatcc gtgaccacct ggaacgtgtc tccgacgtcg ggcactgaat
     8101 ccagtcccag aacctccacc ggcgtggacg gttccgcgtc cttcaattgc tggccgacat
     8161 cgctgaacat cgcccggact ttgccgaaaa cagcgccaca aataaagaag tcgcccacgt
     8221 gcagcgttcc gttctgtacc agcacggtgg caacaggacc gcggccgcgg tccaactgcg
     8281 cttcgagtac cgttcccacc gcgggccgct ggggattggc tttcaaatcc tccatgtcgg
     8341 ccaccagcag aatcatttcg agcagcaggt ccaggttctg ctggctcttg gcggaaaccg
     8401 gcaccatcac cgtctcgccg ccccagtctt cgggcagaag gccgcgatcc gagagctgct
     8461 gcttgatccg ctcgggctgc gcgtccggct tatcgatctt gttaatggcc acgacaatcg
     8521 gcacttcggc cgcccgcgcg tgatcgatcg cttccagagt ctgcggcata acgccgtcgt
     8581 ctgccgccac caccagcacg acgatgtcgg ttaccttggc gccgcgggcg cgcatccggg
     8641 tgaacgcctc gtggcccggc gtatcgatga agacgatctt gcggtcgttc ttcacaatct
     8701 gataggcgcc gatgtgctgt gtaatgcctc ccgcttcccg ctcggcgaca ttcgttgcgc
     8761 gaatcgcatc gagcagcgat gtctttccgt ggtcgacgtg acccatgatc gtgaccaccg
     8821 gcgcgcggcg cgtgagatcc ttttcgtcct cgctctgcac cagtccctgg gtcgcttcct
     8881 cttcgtagct gaccttgttg atggaggcgc cgaactcgcg agcgacgtct tcggcaagct
     8941 tcggatcgag agcctggttg atggtggcga agaccttctt ctccaccagc ttcttgatga
     9001 cgagattcgc tttgaccccc agcttctcgg acaattcctt gacggtaatg ccctcggaga
     9061 tggtgatttc gcggtcgatc ggcggaggcc cttcctgctt gtgcctccgc ggctggaaca
     9121 tcttcccttc gagctccgac tcgcggtcgc gctgcacaac ccgtgctcgc ttgccggtgt
     9181 cgcgccgctg ctggtccgga ggcagagggg aaggcggaat ccccatcgga gaggtcgggt
     9241 gcattggacg ccgccctccg ccgggtctcg cacccggtcc tcccggacgc tgcaccaggg
     9301 cctgacccgg ccggatcggg ccgcgataga tgggctgtcc cggtaccggt gtggtctgcg
     9361 gccgccccgg catagggggc cgcgcctgcg gctggcccgg catcgccttt ggctgtgtct
     9421 gctgctgagc gcggatgcgt tcaattaact tgggatccgg cggcacaacg ggccgggccg
     9481 ccggctggcc ggccagcggc ggccgtgaaa gcggcggcgt ctgtggtgtt gcggccggtc
     9541 tcggcctggc ctgtggcggc catggctgtc ctgagggcgg cgcggcgggg cgcgataccg
     9601 gaggacgagg cggcgcggaa gggcgcggca tcccgggaac cggacccgct tcccccggaa
     9661 atggttgtct tggtcctgtc agaatctggc cgggacgcgg agccggcaga ggcttcgcgg
     9721 gaatcgctcc ggctttctgc cccaccggtg ctacgggtat agacggtgct tgccggggca
     9781 ccggtggcgg aggctcagtg gcggtggaac gcggcgctcc cagaggcgtt tcaaccgcag
     9841 gcggcggagc ggccggctgg ccctcttccg ctgatatctc gggccgtgct ccggcccctg
     9901 ccagcggcgg ccggatcgga cgcggccgtg acgcctgctg caccggcgcc ggcgcgacag
     9961 gctcctcggc tgctgtctcg ggaccgggct cctccgcctg ttcctcgggt ccggaagctt
    10021 ccggtcgcgg gaccgcttcg tgatcctttc ccgtcaattc gggttcttcc acttcgacaa
    10081 cggcattgga tgccgacaga gcatctaaca ctccaggatg atcccccaga ccgcccaatc
    10141 tttgccgcac aatttcagcc acgtcctcgt caatcgagct ggagtgcgtt tttttctctg
    10201 atacaccgag ctcaggcaac agctcgatga tccggtgagg tttcacctcg agctctcgcg
    10261 ccagctcgtt gatacggatt ttcttcatat tttagtactg catgcacaca gacaaaagac
    10321 gtcagaaatg tgggcccgct ggtgtctcag tccgagtctc ttatcgtatc aaattcaact
    10381 tcttgtccgt cccctgggct gctttccgtc tgtccttcgc cactctctga ttccgtagcc
    10441 ggcgccggag gcgcttcgga cgcctcctca gcgatgcctg catcggccac ctcgcttgcg
    10501 gactcctcct cgttcgccgc ggcgggttcc atgctatcca ccgcttcgtc cggcgccatt
    10561 tctacttcag actgcaaggc ggcatccact tgcgcggcct cttcttcacc ttcaaatgcg
    10621 ggctcctcga actgcccata gtaagccacc accgccgcct gaatcttctc aatcaacttc
    10681 gggccgatgc cggcgatctc ttcgagttgc tcgggagtca tcgcccccag ccgctctacc
    10741 gtgccgacgc cgccttccac aagtttttcg acaatgcttt cgccgagtcc gtgatcgatc
    10801 aagaccgaga ccggcgcgcc cgatacaccc agggccgcca tctgcgattc cacttcctgc
    10861 cgcttctctt cctcgctctt gatatcgact ttccagccga gcagtttggc tgcaagcctt
    10921 acgttctggc cctttttgcc gatggccagc gagagttggg aatcgtcgac aatcacttcc
    10981 atgtgccgct cggacggatt cacgatggat acacggctga tcttggccgg gctcaacgca
    11041 tgtgtggcaa acaccaccgg atcttcggag tactcgataa tatcgatctt ctcgccgcgt
    11101 aattcgcgaa tgatcgactg cacccgcatg cccttcatcc cgacgcacgc gcccacgctg
    11161 tcgacgtccc ggtcgcggct gaacaccgcg attttggtgc gttcgcccgc ttcgcgcgcg
    11221 caacccttga tcaccacggt gttgtcataa atctccggca cttcctgctc gaacaggcgc
    11281 atcacgaatt cgggagcggc gcgcgagacg ataacgcctg catttttgcc ggttttttcc
    11341 acgctgcgca tgacacaccg cacccgctcg ccaacgctga agctctccag cctggattgc
    11401 tcctttttcg gcagcctggc ctcggtcttc ccgagatcca taatcaggtc ctgcccttca
    11461 atccgcttca cggtgcagtt gaccagttcg ccaacccggc cggagtattc cgcgtagatg
    11521 ttctcccgct cggcttcccg aaccttttga agaatcacct gcttggcggt ctgcgcggaa
    11581 atgcgcccga gcgcatccat gctcttcgga atgcggatct ccgagccctg ctccgcattc
    11641 gggtcgatgt cgagtgcctc gctcaacgcg atctcccgga ccggatcttc gacggtttca
    11701 acgactttcc gcacggcata aatctggatt gcgcccgtgc gttcattcag ttccgccacc
    11761 aggtcctctt ccgtgcggaa atgcttccgc gcggccacca gcatggcgtc cttgacggcg
    11821 tcgagcacga tgtgcggctc gatacccttt tccttgctca aaatctcgat ggactgatag
    11881 atacttgcca ctctgtcctc tgactctgcg attatccgga gattaccact cgaacttgag
    11941 gtttgctttt tcgatattgt ccagtgtgaa ctgaaccgat ttgccggaag ccggttccag
    12001 cgttacaaga ccttccgaga accccgccag cacgccgtcc cagcggcgcc tctcctcgat
    12061 aggagtgcgc agcgaaacgg ctattttccg gcccagaaac ctctcgaaat ccttcggctt
    12121 cttcaacggc cgttcgagtc ccggtgaact gacttccagg tggtacgatc cccccggtat
    12181 cacatcctcg acatcgagaa tggttccaac ctgcttcgat atgaactcgc aatcgccatg
    12241 cgacacccct tccggtttgt cgatcacgat ccgcagcaaa cgcgccttgc caccaccgcg
    12301 caattcgacg tcgatcacct ccagaccttc cgatccggcg acacgctcag cgatctctgt
    12361 aacctttgct accaccgagt ccttcatgta atgggattct ggcgctcaac aaaaaagtgg
    12421 gctttcgccc actcttggat tcgatctcac cttcattaga ccatgccgga acaaggtggt
    12481 gcaagttcga gacggaatgc agctccgtcc gttcatgcct gtgataagga gtgatgtcac
    12541 tccttcggct tgcgcttcgg ccgcgcggcg ttgatctgtt ttgcctgctc gacagccttc
    12601 tccagatccg catccaccgg ccgtggcttt gccaccgcca gcactacatt gaggctgtaa
    12661 tgcttcaacc ggcgcgtgac ctggcgggag ccctcctgcc gaacttgtaa gtattcctta
    12721 caagttgcct cttcgcctaa ttctccctcc tcgtagatcg cgccgatgtg cagggtgatg
    12781 ttctgcggcg tcgtctggaa aagatcggcg attgccgcct gcgtcagcca aacggattca
    12841 tcctcgaagc ccttagaagc tgtgaagaaa ttgaatgtag ccacggacga acacggatga
    12901 acacaagtga gtttcctgtt ttcaatctgt gtgaatctgt gtccatctgt ggctctgaat
    12961 ctttcacact ttctcacggc cgcggctccg taagttgctg taaatgcatg gctctgatcc
    13021 tgagccgcgc gcgtcagcaa gttccgcatg atccttcggc tcacaacctg agatgaaagt
    13081 gggtgtaggt tgtgatgaga cgcggctgcg gatcgaggcc gtaaaccgca acagggcagt
    13141 gaagcctgcc agcaagtttg gtctgggcac cgcttggaac aacgg
//
LOCUS       DASTMK010000296         6466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_205787, whole genome shotgun sequence.
ACCESSION   DASTMK010000296 DASTMK010000000
VERSION     DASTMK010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205787"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1537
                     /locus_tag="VFQ79_12610"
     CDS             47..1537
                     /locus_tag="VFQ79_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEU0140555.1"
                     /translation="MALSRIFPRTYPKPAASGLVIGMIAVAAAAMGQTAPPVKIGSVT
                     VSGSWRTRVEAWDWFNGAANDAYLFAGSLGRLSFAQQRKTFDWQLEVALPVLLGLPDD
                     ALAAGAQGQLGLGATYYVANNRSRNAALPFVKQAFVRFKNMAGSQAHTLRLGRIEFIE
                     GTETVPKNATLAAVKRDRVAHRLLGNFGWSHVGRSLDAVHYAYNGSRNNVTLLAARPT
                     RGVFQVDGWGGLNIGLFYGALTRQVPMGGSAAEFRVFGLHYEDWRHIVKTDNRPLALR
                     NTDFENIGVTTAGGHYIHAFETRSGTIDLMLWGVLQTGRWGRLDHRAGAATAEAGYQP
                     PGLPRLRPWIRGGFHHGSGDGDPTDGKHGTFFQVLPTPRWYARFPFYNLMNNQDAYLQ
                     LILRPGPKLNLRSEMHWLRLAGRNDLWYQGGGAFQPWTFGYIGRAAGGQRGMAALWEL
                     SADYQWNSHFSLGGYFATARGRDVMNVIYPDGKNANLGCVELGFRF"
     gene            1622..2068
                     /gene="fliW"
                     /locus_tag="VFQ79_12615"
     CDS             1622..2068
                     /gene="fliW"
                     /locus_tag="VFQ79_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HEU0140556.1"
                     /translation="MPSCETKYFGKLDYTESAIILCEAGLPGFESERKFLPLEMPEHK
                     PLVFIQSLSTPGLCFVALPVLGIDPDYRLELAKPDLDALGFESQPAIGTDVLCLALIT
                     ITEASTTANLLAPLVVNIKTGQCVQAIDPESRYSHRHSLDPEEPAC"
     gene            2062..2331
                     /locus_tag="VFQ79_12620"
     CDS             2062..2331
                     /locus_tag="VFQ79_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEU0140557.1"
                     /translation="MLIIRRKEGESILIGEDIEIIVAEAGTRVKLAIKAPSNVPVIRK
                     EVRLVEQQNLDASRTATNAALMKIAGKLSASKQQGTPAPGTDPRA"
     gene            2368..2778
                     /locus_tag="VFQ79_12625"
     CDS             2368..2778
                     /locus_tag="VFQ79_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140558.1"
                     /translation="MSILGAIVRVYSYLFHLVLSLFVLAIAFVTLTSGANTLQMEMLP
                     WKNTALLYWLLALGLIGIIAVVLAVTRKLPILFLIWSVIVFVLLVRGYIFSPYTFNGV
                     SDFSMILLLLLGALIACIGAWLQFRRKTQRRKYA"
     gene            2843..4021
                     /locus_tag="VFQ79_12630"
     CDS             2843..4021
                     /locus_tag="VFQ79_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0140559.1"
                     /translation="MFSSRLDFDLSQNRLSRLLAELRTAGTGVLDLTESNPTRAGFSY
                     PDAEILTSLAEPGSLLYSPEPTGSCTARETIAAHYAAKGLRVRADSVVLTTSTSESYA
                     YILKLLADPGHEILVPRPSYPLFEYLAALESVRIVPYPLVYTGEWSIDFQALERAASE
                     RARAVILVNPNNPTGSFLKQYELEKLIALARSHDLAIVSDEVFADYSFSPDRHRAASL
                     VEVEDTLTFCLSGLSKVSGLPQMKLGWMVVSGPETLRHEALHRLEWIADTYLSVSTPV
                     QHAAPALLAAGESVRAQIHKRSGMNLEFLRTAVNGTPCHLPHVEGGWYAPLQVPRTRT
                     EEEWALDLLRKQSVLVQPGYFYDFESEALLVLSLLTPEDVFREGVWRIVSEVGKEETG
                     "
     gene            complement(4022..5152)
                     /locus_tag="VFQ79_12635"
     CDS             complement(4022..5152)
                     /locus_tag="VFQ79_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140560.1"
                     /translation="MILAAAAMLAAANERAGMLVALDSWNGWQALASAAGWLYAPLGQ
                     SQPAAPDDSTVKALSSLVASKGSVADPARVYLAGRDEAAAAVFYAVSRMPHLWAGAVA
                     IQGDPVAAIETNRLFSANTALIPFLWAAPAEARPVAARLHAAGFPIDFRDVSNVSAED
                     VLQFLNKARQDEFPAKIDCETGNPYFGRCYWLQMENLDASRRNDALPVSRVPPGSGAY
                     LALGGFGYDPRKPGPGVEVGWLPEGYKGPLKIGDTIVSIAGRAIADPAEYRSMMEQVR
                     GPKSTAIMIQRGKQRIRIETRFEMKPREEQFTARVQAEYLTGSREMVLISRGVSRLKL
                     NIPAHWAPAHVNWNGQAQFELGEGGCWLLTDSGTQAGRCDWP"
     gene            complement(5171..5467)
                     /locus_tag="VFQ79_12640"
     CDS             complement(5171..5467)
                     /locus_tag="VFQ79_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140561.1"
                     /translation="MCLTGRLILLAALMLFPGEAQLWNRDRKLPDGRSQKEAILKAEY
                     KRNLKDVAELAQLSAELQTELEENSRHVLSVSSLKKIERIETLAKDIRKRLKRY"
     gene            5532..>6466
                     /locus_tag="VFQ79_12645"
     CDS             5532..>6466
                     /locus_tag="VFQ79_12645"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU0140562.1"
                     /translation="MDDVNVGLVGLGNVGMGAVGILAETGEQIALKLGFRLNVSAVCS
                     RHVDLKTLPAGLGPVFRTNDWRELVSHPEVDIVAELVGGTSTAREIIDAAIEHRKSVV
                     TANKELMALCGAEIWDSAIGAGINLAMEASVCGGIPIHAVLREGISGDRITALLGILN
                     GTSNYILTEIESKGGSFDAVLAEAQRLGYAEADPTADIDGYDARSKLAILAALAFGEK
                     ITPSDIFTQGIRRISPVDFDYAHRLGYTIRLICAARQTPAGLILSVRPSLVSNSTILA
                     GVQGAYNAVWVRGAYGQDTFYYGRGAGSYPTGVAVV"
BASE COUNT         1264 a         1939 c         1904 g         1359 t
ORIGIN      
        1 tgcgccggct gcagagttcc gttttcgtaa gatactggat gacttcatgg ctttatcgcg
       61 tatctttcca cgaacctacc cgaaaccggc ggcatccgga ctcgttattg gcatgattgc
      121 cgtagctgcg gcggccatgg gacagacagc gcctcccgta aagattggtt cagtcacggt
      181 cagcggatca tggcgtaccc gtgtcgaagc atgggactgg ttcaatggcg ccgcaaacga
      241 tgcttatctt ttcgcgggct ctcttggccg gttgagtttc gcacaacaga gaaagacgtt
      301 cgactggcag ttggaggtcg cgctaccggt cctcctcggg ctgccggatg acgctctggc
      361 ggcgggtgcg caaggtcagc tcggtttggg cgcaacctat tatgtggcca ataaccgttc
      421 cagaaatgcg gcgcttccgt tcgttaagca agcgtttgtc cggttcaaga acatggcggg
      481 tagccaggcg cacacgctgc ggctcggccg catcgagttc attgaaggaa cggagaccgt
      541 tccgaaaaat gccaccctcg cggcagtaaa acgcgaccgg gtggcgcacc gcctgcttgg
      601 aaatttcggc tggtcgcacg tgggccgcag cctggatgcg gttcattacg catacaacgg
      661 ctcccgcaac aacgtgacgc tgcttgccgc ccgcccaacg cgtggtgtct ttcaggtgga
      721 tgggtggggc ggattgaata ttgggctttt ttacggcgcg ctgacgcggc aggtccccat
      781 gggcgggagc gctgcggaat ttcgggtctt cggcctgcac tacgaggact ggcggcacat
      841 tgtaaaaacc gacaaccggc cgctggcgct gcgaaacacc gatttcgaaa acatcggggt
      901 gaccactgct ggcgggcact acatccatgc gttcgaaacc agatccggaa ccatcgacct
      961 gatgctgtgg ggcgtgctac agacgggcag atgggggcgc ctggatcacc gtgccggcgc
     1021 cgcaaccgcc gaagcgggtt atcagccacc cgggcttcct cgtttgaggc cctggatacg
     1081 gggaggtttt caccatggca gcggagacgg cgaccccacg gacggcaagc atggcacctt
     1141 ctttcaggtg ttacccactc cccgatggta tgcacgattt cctttttaca atttgatgaa
     1201 caaccaggac gcttatctgc agttgatcct tcggcccggc cccaaactca atcttcggag
     1261 cgagatgcac tggcttcgtc tggctggccg gaacgacctc tggtaccagg gcggcggcgc
     1321 attccagccc tggactttcg gctacatagg acgcgccgcc ggcgggcagc gtggcatggc
     1381 ggccttgtgg gaactcagcg cggattacca atggaacagc catttctcac tgggcggcta
     1441 tttcgccacc gcccggggaa gagatgtcat gaacgtcatc tacccggacg gcaagaatgc
     1501 gaacctgggt tgtgtagagt tgggattccg tttttaaggg ccttcagcgg tcttctcagg
     1561 gatttgctct atagccgcgc aggggaaacg ccgataaccc attcggagac gacagagcac
     1621 gatgccctca tgtgagacca agtacttcgg caagctggac tatacggaaa gcgccattat
     1681 cctgtgcgaa gccggactgc ccggatttga gagcgagcga aagtttctgc cgcttgaaat
     1741 gcccgaacat aagccgctgg tcttcatcca gagtttatcc accccgggac tttgtttcgt
     1801 ggcgcttcca gtgttgggta tcgaccccga ttaccggctg gagctggcaa aaccggatct
     1861 ggacgccctc ggtttcgagt cgcagcccgc gatcggaacc gacgtgcttt gcctggccct
     1921 catcaccatt accgaagcaa gcacgaccgc caacctcctg gccccgctgg tagtaaacat
     1981 caagaccggc cagtgcgttc aggcgatcga tccggaatcc cgatactctc accggcactc
     2041 gctggatccc gaggagccgg cgtgctgatt attcgaagaa aggaagggga gtcgatcctg
     2101 atcggcgagg atatagagat catcgtcgcc gaagccggca caagagtgaa gctcgcgatc
     2161 aaggcacctt ccaatgtgcc ggtgatccga aaagaggtgc gtctggtgga gcagcagaac
     2221 ctcgacgctt cacggaccgc cacgaacgcg gcgctgatga agatcgctgg aaaactgagc
     2281 gcgtcgaaac agcagggtac gccggcgccc ggcacagatc caagagcttg atacaatagg
     2341 tcgtttccag ttggaggctt ttgtttcgtg agtattcttg gagcgattgt gcgcgtatat
     2401 agttatctat tccacttggt tctctcgctt tttgttctgg ccattgcatt cgtgaccctc
     2461 acaagcggtg cgaatactct gcagatggag atgctcccgt ggaaaaacac cgcccttctg
     2521 tattggctat tggcacttgg gctgatcggg attattgccg ttgttctcgc cgttacacgg
     2581 aagctcccca tcctgtttct gatctggtcg gtcattgtat tcgtgctcct ggtgcgaggc
     2641 tatatatttt cgccgtacac cttcaacggg gtgtccgact tcagcatgat tctccttctg
     2701 ttgctgggag cgctcatcgc ctgcatcggc gcgtggcttc aattccggcg aaagacgcag
     2761 cggcgcaagt acgcctgagc atcacacctt atccatccgg gctacttccg cgccggcgat
     2821 cgagaacgac gagcgccatc gcatgttttc ttcccgtctc gatttcgatc tgagccagaa
     2881 ccggctctcg cggctgctgg ccgaacttcg taccgccggc acaggcgtac tcgacctgac
     2941 ggagtcaaac cctacccgcg ccggcttctc ctatccggat gccgaaatac tgacctcact
     3001 cgccgaacca ggttcgcttc tctacagtcc ggagcctacc ggaagctgca cggcacgcga
     3061 gacaatcgcc gctcattacg ccgcgaaagg tttgcgtgta agagccgatt ccgtggttct
     3121 gacaaccagc accagtgagt cttatgcgta catcctgaaa ctgctggcgg atcccggcca
     3181 cgagatcctg gttccgcgtc cttcctatcc gctgtttgag tatctggcgg cgctggaatc
     3241 agtgcgcatt gtgccgtatc cgttggtcta taccggcgaa tggtccatcg atttccaggc
     3301 gctcgagcgt gccgcctcgg agcgcgcgcg agccgttatt ctggtgaacc cgaataatcc
     3361 aaccggctcc ttcctcaaac aatacgaatt ggaaaagctg attgctctcg cccgatcgca
     3421 tgatctggcg atcgtctccg acgaggtgtt cgcggattat agcttctcgc ccgacaggca
     3481 tcgcgcagcg agtctcgtag aagttgagga tacactcacg ttctgcttga gcgggctgtc
     3541 caaggtcagc ggcctgcccc agatgaaatt gggttggatg gtggtctcgg gtcccgagac
     3601 gctgcgccac gaagccctgc accgcctgga atggatcgcc gatacgtatc tttccgtcag
     3661 cacaccggtg caacatgcgg cgccggcgct gcttgccgcc ggtgaatccg tccgggcaca
     3721 gatccataag cgcagcggga tgaatctcga gttcctgcgc acagcagtga acgggacccc
     3781 gtgtcacctc ccgcacgtcg aaggaggctg gtacgcccct ttgcaggttc cgcgtacgcg
     3841 cacggaggaa gagtgggcgc tggaccttct ccgcaagcag agcgtgctgg tgcaaccggg
     3901 gtatttctac gacttcgaat ccgaagcgct ccttgtccta agccttctga cgcccgaaga
     3961 tgtttttcgg gaaggagtct ggcggatcgt ttcggaagtg gggaaggaag aaactgggta
     4021 actacggcca gtcgcagcgg ccagcctgtg ttccgctgtc ggtcagcagc cagcaaccgc
     4081 cctcgccaag ttcgaattgt gcctgcccgt tccagttcac gtgggccggc gcccagtgcg
     4141 ccgggatgtt cagcttcagc cgcgacacgc cgcggctgat cagaaccatt tcccgcgatc
     4201 cggtcagata ttcagcctgc acgcgcgccg tgaactgttc ctcacgtggc ttcatctcga
     4261 accgggtttc aatccgtatc cgctgcttgc cgcgctgaat catgatggcg gtggacttgg
     4321 gtccgcggac ctgctccatc atcgaccggt attccgccgg gtcggcaata gcgcgcccgg
     4381 cgatggagac gatcgtgtca ccgatcttta aaggaccctt gtagccttcc ggcagccatc
     4441 cgacttcaac accgggcccc ggcttgcgcg gatcgtatcc gaacccgccg agcgccagat
     4501 aggctccgct gccgggcggc acgcgggaca ccggaagggc gtcgttccgg cgcgaggcat
     4561 ccaggttttc catctggagc cagtagcatc ggccgaaata aggatttccg gtttcgcagt
     4621 cgatctttgc agggaactcg tcctgacggg ccttattcag aaattgcagc acatcttccg
     4681 cggagacgtt cgatacgtca cgaaaatcga ttgggaatcc cgccgcatgc agccgtgcag
     4741 ctaccggccg ggcctccgcc ggtgcagccc atagaaacgg aatcagcgcc gtgttcgccg
     4801 agaacagccg attggtttcg atagcggcta cgggatcgcc ctgaatggct acagctccgg
     4861 cccaaaggtg cggcatgcgg gaaacggcat aaaaaacggc ggcggcggcc tcgtcccgcc
     4921 ccgcaagata cacgcgcgcc ggatcggcaa cggacccttt ggaggctacc aaagagctga
     4981 gagccttcac cgtggaatcg tccggagcgg ctggctggga ttgccccaaa ggcgcataca
     5041 accatcccgc ggcggatgcc agcgcctgcc atccgttcca ggaatcgagc gccactaaca
     5101 tccctgcacg ctcattcgcg gctgccagca tggctgcagc cgccaggatc atcaccgctc
     5161 tcaacatgcc ctagtatcgt ttcagccgct tccggatatc tttggcaagc gtctcaatgc
     5221 gttcgatctt cttgagtgag gagaccgaaa gcacgtgccg cgaattttct tccagttccg
     5281 tctgaagctc cgccgacaac tgggccagtt cggcaacatc cttcaggttc ctcttgtact
     5341 cggctttaag gatcgcctct ttctggctcc tgccatccgg cagcttccgg tcgcgattcc
     5401 agagttgggc ctctccgggg aacaacatga gagcggccaa taaaatcagc cggccggtta
     5461 agcacatcgt atttgtatac ttagcacacc aggggcaggg taagtctacc cgcttgttat
     5521 catgaccctt tatggatgac gtgaacgttg ggctggtggg cctcggtaac gtaggcatgg
     5581 gagccgttgg aattcttgcc gagactggtg agcagattgc gctcaagctt ggttttcgcc
     5641 tgaatgtcag cgcagtgtgc agccgccatg tcgatctgaa gaccttaccg gccggccttg
     5701 gcccggtatt ccgcaccaac gattggagag aactggtatc gcatccggaa gtcgacatcg
     5761 ttgccgaact ggtgggtggg acgtccacag cccgtgaaat catcgatgcc gcgattgagc
     5821 accggaaatc ggtggtcacc gccaataagg aattgatggc cctctgcggg gcagagatct
     5881 gggacagcgc catcggcgcg ggcatcaacc tcgccatgga agcaagcgtc tgcggaggca
     5941 tccccatcca tgccgttctt cgcgaaggca tctccggcga ccgtattacg gcgctgcttg
     6001 gaatactgaa cggcaccagc aactatatcc ttaccgagat cgagagcaag ggcggaagct
     6061 tcgacgccgt tctcgccgag gcacagcggt tgggctacgc ggaagccgac cccaccgccg
     6121 atatcgacgg ctacgatgca cgctccaagc tcgcaatcct ggccgcgctt gcgttcggcg
     6181 aaaaaataac gccgtccgat atcttcacgc aaggcatccg gcgcatttcc ccggtggact
     6241 tcgattacgc acaccggttg ggttacacca tccgcctgat ttgcgcagcg cggcaaacgc
     6301 cggcaggact tattctatcg gtgcgcccat ctctggtctc gaattccacg atcctggccg
     6361 gggttcaggg tgcgtacaat gccgtctggg tgcgcggcgc ctacgggcag gacacttttt
     6421 actatgggcg cggcgcgggc tcttacccga ccggggttgc cgtagt
//
LOCUS       DASTMK010000297        24559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_238060, whole genome shotgun sequence.
ACCESSION   DASTMK010000297 DASTMK010000000
VERSION     DASTMK010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24559
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238060"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFQ79_12650"
     CDS             <1..454
                     /locus_tag="VFQ79_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0140563.1"
                     /translation="ALELHWTGARVTLIHRGGGISGSVKYWIKPNIENRIKSGEIKAY
                     FHSSVREITVDSVLLATPEGEVRLKNDFLFAMTGYRPDLEFLSALGIVLEPATLRPRT
                     DAETLESDRPGIYLAGVIVAGMHTNEIFIENGRFHGRKIAEAIAAALN"
     gene            complement(481..1542)
                     /locus_tag="VFQ79_12655"
     CDS             complement(481..1542)
                     /locus_tag="VFQ79_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140564.1"
                     /translation="MKLLLAVLGLCLGAFAQMKMTVAQLHSFVKSSVELRHEDRRVAE
                     YLRKTSLTERLDTRAMDDLRALGPGPRTLVALEELQERSKALPAPPPVRGKEVIAVIE
                     PPSSAAQKKLINDTRNYAMEYVKRLPDFICTQVTRRYVDPTGLEFWQRADVVTARLTY
                     FEQKEDYKVVLVNNTATETGFEKLGGATSTGEFGSMLREVFDPKTDTAFRWERWATLR
                     GRRMHVLAYQVAKRHSAWGIRFDKVEEITPGYRGLVYVDRDTGSVMRITLEAVDIPPS
                     FPIQQASQTLDYDLIDISGTNFVLPLKSVMRMRHGRFLVKNETEFRMYRKFGVEAVIT
                     FDTPEPLPEAATEEQPPKP"
     gene            complement(1587..2282)
                     /locus_tag="VFQ79_12660"
     CDS             complement(1587..2282)
                     /locus_tag="VFQ79_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU0140565.1"
                     /translation="MLEITWLGHSTFQFRLDTGEVIVMDPWIEGNPARPPDHKFDRVD
                     VICVSHGHFDHIHDAVALAERFSPQIVGIFETCHWLESKGVRNTNGMNKGGSQKVGPV
                     TFTMTHAVHSCGILDDGKILYGGEAAGYVLHLPDNRKLYFAGDTNVFTDMQLIHELYR
                     PEIAFLPIGDLFTMSPREAAVACRLLKPPKVVPMHFGTFPALTGRPEQLAELLKCTDT
                     EVWPLEPGKPVEW"
     gene            complement(2328..3341)
                     /locus_tag="VFQ79_12665"
     CDS             complement(2328..3341)
                     /locus_tag="VFQ79_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140566.1"
                     /translation="MARVFAVLRALTRAFFRDQKSLGSVAGNNFFLVTALLLQNAGGF
                     LYLLIALVLLFPLSTDPLRKIPASRLVFWPLEKRDRWMLRVVSPWVNPVSWLVAVLAI
                     WTVRGSFTTGLWILIAGLFAAAFCLSDLPFSPYRALWRRIPNFPGRWNQLIRKNIREM
                     LMTLDFYCALLLSIAAVIFRLTVADPPREALLALTVLIVLALSSYAQCLFGLDGDGGL
                     LRYQLLPLRGWEILAAKDAAFLFLVIPLTLPLAPLSGTAAALIALAMGHQPAVYQPRE
                     QARGRFSSGASLLFGLAQAVLMTTAAAGVFFNSVLVVVPCLAVWAGSVAYYGRELERA
                     SRA"
     gene            complement(3317..4144)
                     /locus_tag="VFQ79_12670"
     CDS             complement(3317..4144)
                     /locus_tag="VFQ79_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0140567.1"
                     /translation="MRAISPSPFRISLPKARAFRFPRGSNNVMIRCHNVTRRFGNFTA
                     VDGLTFDIAPGTICAFLGPNGAGKSTTIKMLTGLLAPSSGEIRVCGLDVKSAARELKQ
                     RIGVLPEGLGPFDDLTVEEHLLLTGDVYGLDRRETRARTSQLLHALSLEEGRDTFASS
                     CSHGMRKKTAFAMALLPNPRVLFLDEPFEAIDPVTSKIMRDLLQYVARRGVTVFLTSH
                     ILSVTEQIATQFILIRNGRIVWNSPAEELPQSLEQHYFDLVEAPAVEELQWLGSSPS"
     gene            complement(4065..5831)
                     /locus_tag="VFQ79_12675"
     CDS             complement(4065..5831)
                     /locus_tag="VFQ79_12675"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEU0140568.1"
                     /translation="MTIHSKLAVATIAALAAFSYSGSSAETQAQTPAHLERWRSGMTL
                     RERIAQLVVIPFYGDLPGPKSAEHKRFLHLVAESRVGGLILVNRVQNGIAKNAEPHEM
                     ASFINRMQKVTKVPLIVAGDFERGASMRVSGTTKWPHNMAFGAARDYEASHAQGAYTA
                     EEARALGVHWVFAPDADVNNNPANPIISIRSYGENPQEVASHVRSYIEGARSAVHPVL
                     VTAKHFPGHGDTAVDSHVGLPRLEMARTRMEEVEFTPFRAAIDAGVDAIMTAHMSVPA
                     LDPSDTPATVSHAILTGVLRKELGFQGIVVTDAMDMQGLTKILPGGEAAVRAIEAGAD
                     VLLMPPDPDVAVAAVLKAVNSGRISRQRIEESLERVLAAKLRVGLGENSLVDLKNISR
                     VVNSPESQDLAQQVAGRAITLVKNQDDILPLENPASACHFILSESRLGQSGRKLAELM
                     PKELPGASWHRFTPATPAAELSKAAKKAAACKAVVLYAFVSVAAYRGNVALAGGYPKF
                     VEQLIAGPAPVVMVSLGNPYLLSSFPDVAAYLNTYSTAPTSEVAALKAIKAEIPIEGH
                     LPVTIPDIAPQGAGIPLPARLQ"
     gene            complement(5965..6414)
                     /locus_tag="VFQ79_12680"
     CDS             complement(5965..6414)
                     /locus_tag="VFQ79_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0140569.1"
                     /translation="MNADISLTPPPAHILIVNANRHGLAARRSVLEELGYTVSIAKGA
                     EEALHAFARKTFSLVVTDYKMPDMNGVELIREIRREHPAMPVILISGFADTLGLNESN
                     TGADVVIQKSANEVIHLVRSVKRLLSAPKKPVRRQQFSLRAKRHGVQ"
     gene            complement(6516..7643)
                     /locus_tag="VFQ79_12685"
     CDS             complement(6516..7643)
                     /locus_tag="VFQ79_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0140570.1"
                     /translation="MRFARSYCLMPALLLLAGTVVLSAQEGPRKESSETVARPRTSKP
                     EEAKQQEKIPSEFKKDKEDVAPTASFKSDVLTVDLDVAVMDNRGNFIPQIPRGNFRVL
                     EDGVPQQVTNFSMGEAPMTVALVIEFSNLFQNYWGETWYQTLTAAYGFLETLKPEDYV
                     AVVAYDMRPEILSDFSTDKRQAYDAMQRLRIAAYSESNLFDAITDTADRMSEIEGRKA
                     IVLISSGIDTFSKLTFDKTRKALQTAGVPIYAIGLMQALREWYDARGYLGSIARLDFL
                     QADNQLRTFANETGGQAYFPRFYGEFPSIFGSIAQALRNQYSLAYQPTNQARDGKYRK
                     IKVELVNPATNEALRVTDQKGKSIKYKIIAKAGYTAPREVE"
     gene            complement(7737..8501)
                     /locus_tag="VFQ79_12690"
     CDS             complement(7737..8501)
                     /locus_tag="VFQ79_12690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0140571.1"
                     /translation="MRQTYQGYGGFASLGGRFPPGVKWLLIVNTALFVLYFFAVRTGL
                     GGIFYPFGLVPVAVLKLFGIWQLFTYMFLHDPYGFTHILFNMLMLWMFGTDLERTWGT
                     RGFLRYYFICGVGAGICIVIGNLLFGTLETRTIGASGAIFGLLLAFGMLFPNSTVLFW
                     FLFPIKAKYFVMIMGAIQFMLTLGSTGSGVSHVAHLGGMIFGYGYLKWGRGARVRTNF
                     AGDLFRQYNRWKMQRAKRKFDVYMRKRDSNRGPWVN"
     gene            8607..9833
                     /locus_tag="VFQ79_12695"
     CDS             8607..9833
                     /locus_tag="VFQ79_12695"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEU0140572.1"
                     /translation="MQSRRTFLAGSLLAYAGTRPLRGAARSYAEIEKLLARDDIKGKL
                     SKSELPTPALIVDLDAFEHNVASMSAHAKASGRALRPHAKTHKCPEIAKILIKAGAVG
                     ACAAKISEAEALARDGVTGLLVTTAVIGPHKIERAVRLTSRRPETILSVDNAQNVRDL
                     NDAAGAARQKLNLAVDLWISNRTGIRPGEPALALAQLIDSLPHVKLAGIQAYAGNASH
                     TKGFEQRKKFSREVMAQAVDTRRLFESHGIQCPLLTGGSTGTYNIDSEIEGITELQPG
                     SFIFMDIDYNRIGGKDGDVYQDFRNSLFVTATVVSKPTDSSAIVDGGLKAFATDRGYG
                     PSLRKGEGTPYAFAGDEHGRLSLADSGSSVNVGDRLDFVVPHCDPTVNLYDRIYGVRG
                     DAVECAWRITARGMSQ"
     gene            complement(9840..11168)
                     /locus_tag="VFQ79_12700"
     CDS             complement(9840..11168)
                     /locus_tag="VFQ79_12700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU0140573.1"
                     /translation="MKRLVVVGTGYVGLVSGAMFASLGNRVTCVDKDPSIVEALRAGR
                     STIHEPGLDEILQSTIADGTLSFTTETAEAVAGADATFIAVGTPSLESGAYDFQYVEA
                     AAHEIGRALRRPKRHIVVLKSTVTPDIYTRVQTILAGETEGSGAQIEVVSNPEFLAEG
                     TAIRDFSQPHRIIVGATSPAARQFMEGLYRPFTKRKPGVLQLTDPKSAIVTKLAANSF
                     LACRVGLINELARYCDAVSADIEHVRTGLASDPRIGHLFLYPGPGYGGSCFGKDLRAL
                     RESSREHGADLSLVPAIEESNLRHKIYLPRRIERQLAPLAGRTLAVWGIAFKARTDDI
                     RESPAVDAIEYLLERGARIRAHDPEAGQRAAERFGSRITLCGNKYDAVDGAAALVIMT
                     EWDTYKSPDYHELHERLSGRIVFDARNILDGSEVRQAGLTYVGMGRGCAT"
     gene            complement(11240..12394)
                     /locus_tag="VFQ79_12705"
     CDS             complement(11240..12394)
                     /locus_tag="VFQ79_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0140574.1"
                     /translation="MPENCRGIDSTTGAVIEISFDRVIQSVDHVIGNGGTHSYVSAGW
                     IDLQVNGFAGVDYNTPSTPHEEIARSIRVLHSTGITRFLPTVITGAPDDMAGALGNLA
                     RARESLEEGPAMEGFHVEGPHISPEDGPRGAHPKAWVRKPDLDEFRRWQDAARGNVRL
                     VTLSPEWPEAVGYIEALVREGVVVSIGHTKAGPREIADAVSAGATLSTHIGNGAHAVL
                     QRHPNYIWEQLAEDRLTAGFIVDGIHLGAAFLKVGLRAKGVERAFLVTDAVMPAGCAP
                     GRYKLGGVEVELHPDDRVTLVENTRLAGSALRMERGIENLMKLAGLSLTEAITMATRN
                     PARAARIAARQRGLTPGERGDVVQFRFDPDSKRIEVEKTWVSGKEMYKAA"
     gene            complement(12605..13552)
                     /locus_tag="VFQ79_12710"
     CDS             complement(12605..13552)
                     /locus_tag="VFQ79_12710"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU0140575.1"
                     /translation="MLTPTEYIWHNGRFIGWNDAKIHVLSHVVSYGSSVFEGIRCYST
                     PSGPAIFRVREHVRRMIDSAKIYRMDNLGYNAGQLVDAMVELVRVNKLDACYIRPIVL
                     RGYGEVGVHAGNNPLEIYIACWEWGKYLGEEALAQGVDVCISSWNRLAPNTLPALAKA
                     GANYMNSQLIRMEAQINGYSEGIALDEAGYVSEGSGENIFVVRDGRILTPPLGASVLP
                     GITRDSVIKLAEDLGLPVIETIVPREMLYIADEVFFTGTAAEITPIRSIDRIVIGSGK
                     RGPVTEKLQQEFFEVINGKKPDSYGWLTKVNAGQPVAAN"
     gene            13630..14502
                     /locus_tag="VFQ79_12715"
     CDS             13630..14502
                     /locus_tag="VFQ79_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU0140576.1"
                     /translation="MKRRLFSLVLLSSLVCPALLDARDLKPGWNLFSKEQDVQLGKEA
                     AAQVRQQMPIVKEADINDYIRRIGQRLASQPEAGDFPYSFEVVADKSINAFALPGGPA
                     FVHTGLILAAENEAQLAGVLAHEIAHVALRHGTNQVSKANAVQIPAMILSGVLGRGGS
                     MMDQLTQLGIGIGANSVMMKFSRSAERDADLLGTRIMSGAGYNPIEMARFFEKLEAEG
                     GSRGPEFLSSHPNPGNRVKAVQAEIQTLPRKQYTTDTGDLSRIQSSVSKIRVEPARTT
                     PARSTPLRSRQRSR"
     gene            complement(14507..17446)
                     /locus_tag="VFQ79_12720"
     CDS             complement(14507..17446)
                     /locus_tag="VFQ79_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0140577.1"
                     /translation="MTHRFCLLSLAAALLAQGTTSPAAIVESPRRVPVADTVDVVVAG
                     GGIAGVSAALEAAAQGRSVLLIAPRLYLGEDLADTMRLWLEEGEKPVGALTETIFSKP
                     GPAPPLRLKKTLEDALTKAGVRFLLGCTATEILLDGKGQPAGLVMANRAGRQAVIAKV
                     IVDATAHASVAEQAGAKFHPWAGGTVEFQRVVLGGKPVNEDAIRRRVPMPDQNQDYLE
                     YSLRLNLPDTGSLSFGRVEQEARDLTYRPGQMRASARLHFVYPNGIIGRRPASAWQGF
                     TRPHIGHFQPRGIDRLYVAGSRADLDRDAAELLMRPTVAEAVGRFIGADAARQAASLS
                     SPKAPQVASSVLRTGTSAGEIREVLSGLRPTEPGTGYVNSPARALPILGEYDVVIIGG
                     GTSGAAAAIGAGRRGAKVLVAEFQEGLGGTGTLGMIGKPYHGRNEGFAKEVPFPSETF
                     TIDDKMEWYRKEIRKTGGEILFGVLGSGAIMEGNRVRGAVIVTPQQRGAVLAKVVIDA
                     TGNADVAVSAGAKAMFGSDAEDLAMQGAGLPTRKPASSYVNTDYLLVDENDMLDVWRA
                     LVGARKTMTEDAYDSGALIQTRERRRVVGEHILSYLDQIAGRTYPDSIGYSASDYDSH
                     GYPSEDLFALLPHDQRSRLANHPAPGGACYTPYRCLLPRGLEGILVAGLGISMHRDAS
                     AMVRMQRDMANQGYAAGVAAAMTAASGRPLRDLDVRSLQRHLVGIGSLPAAVLEHQDS
                     FPLPDSEIRQAVQNVAFATNPDEAGVWLAVILTHKEAALPLLRKTFETARPRHKLTYA
                     NLLAFLGVPEVAPVLMEALDAIQEWDPRILQGRMAEYAHLPTPIDTLILGLGRTGDRK
                     AVPALLRKLETLDASVALSHHRAVAIALEQLADPAAAEPLARLLAKPGMRGHVMKELE
                     PLHDADMDRRRRLGPMREITIARALYRCGDYKGLGEAILKDYTADIRGFFARHATAVL
                     STPVQFQ"
     gene            complement(17461..20874)
                     /locus_tag="VFQ79_12725"
     CDS             complement(17461..20874)
                     /locus_tag="VFQ79_12725"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0140578.1"
                     /translation="MRRNTTLIWMALLCAVPAWGQSARITGIVTDTSDAVMSGVEITV
                     TNTSTGVSRRAVSNESGNYSVPLLQPGPYRITAQASGFKPLSRDGINLVVDQVARIDI
                     QMEVGQVTELIEVVSDATMVDSSNATLGVVVENRRVVDLPLNGRNALALVMLTPAVIS
                     QAGPTNSGFANRGTALSAVSINGGPGVFNNYVLDGGNNNQAQQADINVQPAVDAVEEF
                     KVQHNSMSAEYGFTLGGVVNVVTKSGTNDPHGVLYHFLRNDKFDARNTFADTRAPFRY
                     NQFGGSLGGPIVLPRIYSGMNRSFFFTNYEEWRYRRYENPIIRVPTAAEREGDFSNLR
                     AASGALIPIFDPLTTRANPSGSGFIRDPFAGNRIPQNRIDPVSRNILPFYPLPNRAPS
                     DPFTNANNYLSTIDETQSMRQLTSKLDHRISESNSIYGRYTYYRHRTDGGVGGSPYPD
                     PLVRQRIDNFVTHNLIINNTHTINPATINEFRAGLARSYFPFESLSYGKSIPQQLGLP
                     SSVPPDTFPSISNGMTSFGTFYAGLRGTVIFQVHDMVTLTRGNHTLKAGGEWRKLQAN
                     NLNNTNPSGSYAFAAGLTGNPQQPGGTGFGFATFLTGSVSSASLTRYLGASNLGSSIS
                     GFIQDDWKVTRRLSLSLGLRYDYQTWPIDRYDGISNFNPLALNPENNLIGRMEYAGVD
                     FGRSVREPDYNNWGPRLGFALDITGRGATVLRGGYSIFYPQLVNNSFYGNTAGFATAT
                     TDYQPPGNNTNFPAFQFSDGLPSPPIAPLGAALGPSAFLGQNVTYEESNAQVPMSQQW
                     GLSLQQRLKGWRFEISYSANRVSHMAAGNYDLNQLDPQHLALGLALQNQVANPYAGIV
                     PGSLGNATITRLQSLRPLPYYNQVAVFRPTLGSSIYNAMLLSVERRLSNGFSILLSYT
                     NGKLLSDSIQTTSIGSEQGGVTNYQNGKFNRAAEWSLDPTDVSQRLVISGLYELPFGR
                     GKHWSFSHGVLDGFARGWQANIISTMQSGLPILVRGANNFLADRPNSTGASAKLDNPT
                     AARWFDTTQFVNPPNFTFGNVGRVLPDVRSPGLVNFDFSLMKDTYVRERVRLQFRAEA
                     FNVFNQVNLRFPNATFQPGTVGTNISGNFGVITSARDARIMQLGLKLVF"
     gene            complement(20896..22296)
                     /locus_tag="VFQ79_12730"
     CDS             complement(20896..22296)
                     /locus_tag="VFQ79_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140579.1"
                     /translation="MAKNWWSALGAEKHLTEEILLQHIDGALSRKEDDAAARHLRDCW
                     ECRTLLERLDSAIHAFMEERRDQFRTLEGTPHSSNGFSARLARHAALLKPESPQSGFR
                     IFFLARRVFWPLAAAAAALAGVGAWPPVRDYVFRSTVPEARQPAPRPSAVRTPPPTAA
                     PAPAPPEAAPLAAVTVPRIPKTQPPLLFVPPTSAELDAAEFAAYLALHEAGLCRGNTI
                     EVLRVPGDSIRITGVADRLQQAENLRAALAGVKLADVRVAIPSEDLSPVAADAAPTLL
                     IEPRPPLLADRLKEHFRRLSPPERAGKDMADYSSQTVRLAGIAHQEAQALRTLATVFT
                     AHRIEAMKPEDRVRFLRLVEEHLQELRHGLTQLGQLLDQLDYDASTAAMEPAPVMGHW
                     EQSGVLLERQTARMDYLIGGLFAGLDLGGVDAGIAWRELRELHQQSLELYRTMDVIAR
                     AGWDPDRLKDFGLTGR"
     gene            complement(22289..22840)
                     /locus_tag="VFQ79_12735"
     CDS             complement(22289..22840)
                     /locus_tag="VFQ79_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0140580.1"
                     /translation="MDDTLTGSDYLLSKRSDSHLVEEWVCAQYEKLGPGLRRYLLRLT
                     GDPGHAEDFVQETFLRLYEEVLKNRKVKRLRPWIFQVGHNLAIDFLRRRGQEEWVHEE
                     GRDTEYPDTAPSAEAAMLAAERHRQIRDGLYLLSPQERQVLELRAEGLLYREIAGLMG
                     LQVSTVTTCLSRAVQKIARQIHG"
     gene            22944..23573
                     /locus_tag="VFQ79_12740"
     CDS             22944..23573
                     /locus_tag="VFQ79_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU0140581.1"
                     /translation="MSAYDAILFDFDGVLVDSEPVHFTCWRDVTAPFGVKLDWDSYLK
                     ELVGITDREAVEWMASHSDPPVDPEKLWALYPAKKAMFRERMLAAPPFAPGLAEFLEG
                     ADSYKLGVVSSSARSEVEPLLEAGGLRRYFDTVVCGEDVTRHKPAPEPYLLAAGRVQA
                     QRPLVVEDSNPGIRSGRAAGFDVVQVRDVSTMCQLVAERLQDGRITKQG"
     gene            complement(23557..>24559)
                     /locus_tag="VFQ79_12745"
     CDS             complement(23557..>24559)
                     /locus_tag="VFQ79_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140582.1"
                     /translation="LVQQGQVVVKGAALVELESIDAVTDLAAAEARARQASAELEIRQ
                     KGGSAPELAAIESSMKEARLEVQAARREYESLERLQAKKAATAQELAVARDRLAQAEA
                     AIEALERKRASLVVESDIAVAEARVRDAQIALDAARRRVEQGTIRSPISGTVYSLSVR
                     VGAFVNPGDELARIGKLDKMRVEIYVDEPELGRVREGLPVSITWDAKPGQTWTGEVER
                     VPTEVITLGTRQVGTVIAVVDNANGELLPGTNVNAAIRSHVVENALVIPKEALRHREQ
                     ENGVFVLHDGRVQWRPVEIGASSVTEVQVTAGLAEGDRVALATERPLREGEPVNPVS"
BASE COUNT         4915 a         7403 c         7441 g         4800 t
ORIGIN      
        1 cgccctggaa ttgcactgga cgggcgcgcg cgtcacactc attcaccggg gaggcggcat
       61 ctcgggttcc gtaaagtact ggatcaaacc caacatcgag aaccgcatca agagcggtga
      121 gatcaaggcc tactttcatt cttcggtcag ggagatcacc gtggattccg ttcttctggc
      181 gacgcccgag ggtgaggtac ggctgaagaa cgatttcctg tttgcgatga cgggttaccg
      241 gccggacctc gagttcctgt cggcgttagg catcgtgctg gaacccgcga cgctgcgtcc
      301 cagaaccgac gccgagacgc tcgagagcga ccgtccggga atctatctcg ccggagtgat
      361 cgtcgccggc atgcacacca acgagatctt catcgagaac ggccgcttcc acggcaggaa
      421 gatcgcggaa gcaatcgcag cggcgctgaa ttgagccgga gctgagccac gcgcgagcgg
      481 ctacggcttc ggaggctgct cctcagtggc ggcctccggc agaggctcgg gagtgtcgaa
      541 cgtgatgacg gcctcgacgc cgaacttcct gtacatccgg aactcggttt cgttcttcac
      601 caggaaccgg ccatgccgca tccgcattac ggacttgagc ggcaggacga agttcgtacc
      661 gctgatgtcg atcagatcgt agtcgagcgt ttgcgaagcc tgttggatgg ggaacgaagg
      721 cggaatatcg accgcttcca gggtaatccg catgactgag ccggtgtccc ggtcgacgta
      781 caccagaccg cggtaacccg gcgtgatctc ttccactttg tcaaaacgaa taccccacgc
      841 cgagtggcgc ttggccacct ggtaagccaa cacgtgcatg cgcctgccgc gcagtgtcgc
      901 ccaacgctcc caacgaaacg cggtgtcggt cttcgggtcg aagacttccc gcagcatgct
      961 gccgaactcg ccggtcgatg tcgcgccgcc cagtttttcg aagccggtct ccgtggccgt
     1021 attgttcacc agaaccactt tgtaatcttc tttctgttcg aaataggtga gccgggccgt
     1081 cactacatcg gcgcgctgcc agaattcgag cccggtgggg tcgacgtagc ggcgggtgac
     1141 ctgagtgcag ataaaatcgg gcaggcgctt gacatattcc atcgcgtaat tacgcgtgtc
     1201 gttaatcagc ttcttctgtg cggcagagga gggcggctcg atcaccgcga tcacttcttt
     1261 gccgcggact ggcggcggcg cgggtaaagc tttcgaacgc tcctgcaatt cttcgagggc
     1321 caccagtgtt ctcgggcctg gaccgagcgc acgaaggtcg tccatcgcgc gcgtgtccag
     1381 ccgttcagtc aacgatgtct tccgaaggta ttcggcgact ctccggtcct catgccgcag
     1441 ctccacggac gacttcacaa aggagtggag ttgcgccact gtcatcttca tctgggcgaa
     1501 cgcccccagg cataacccca gcacggccag gagcaacttc actcgcataa ctctatttta
     1561 gacggagccg gaacgctttc cggttcctac cactcgaccg gcttgccggg ctccagcggc
     1621 cacacctcgg tatccgtgca cttcagcagt tcggccagtt gttccgggcg tccggtgagc
     1681 gcagggaatg ttccgaaatg catgggaaca acttttggcg gcttcagcag acggcatgcc
     1741 acggcggcct cccgcgggct catcgtgaac agatcgccga tgggcaggaa tgcgatctcg
     1801 ggccggtaca attcgtgaat caattgcatg tcggtgaaca cgttcgtatc gcccgcgaaa
     1861 tacagcttcc ggttgtcggg cagatgcagc acgtaacccg ccgcttcgcc gccgtagagg
     1921 attttgccgt catccagaat gccgcaacta tgcacggcgt gcgtcatcgt gaacgttacc
     1981 ggaccgactt tttgcgaccc tcccttgttc atgccgttcg tgttccggac tcctttcgac
     2041 tccagccagt gacacgtctc aaagatgccc acaatttgcg gagaaaagcg ctccgccagc
     2101 gcaaccgcgt cgtgtatatg gtcgaagtgc ccatgagaaa cgcagataac atccaccctg
     2161 tcgaatttgt gatccggcgg gcgggcggga ttgccctcaa tccacggatc cattacgatc
     2221 acttctcccg tatcgaggcg aaactggaat gtagagtgtc ccaaccacgt gatttcgagc
     2281 atttagtatt gcctcctcaa gagatatcct aaaggtttcc cggggcatca cgcgcgagaa
     2341 gcacgctcca actcgcgtcc gtagtaggca acggatcccg cccagaccgc caggcacggc
     2401 accactacca ggacgctgtt gaaaaagacg ccggcggcgg cggttgtcat cagcactgcc
     2461 tgggccaggc caaacagcaa cgatgcgccg ctcgaaaaac gtccgcgtgc ctgttcgcgt
     2521 ggctggtata ccgcgggttg gtgtcccatc gccagcgcga tcagagcggc ggctgtgccg
     2581 gacagcggcg cgagcggcag agtgagggga atcaccagga acagaaacgc cgcgtctttg
     2641 gccgccagaa tttcccatcc tcgcaacggc agcagttgat aacgcaaaag gccgccatcg
     2701 ccatcaaggc cgaacagaca ctgcgcatag ctcgaaagcg ccagaacgat gagcaccgtc
     2761 agagccagca atgcttcccg cggcgggtcg gccacggtaa ggcgaaagat gacagccgca
     2821 atgctcagca gcaacgcgca gtaaaaatcc agcgtcatga gcatctcgcg gatattcttc
     2881 cggatcaact ggttccaccg gccgggaaaa ttgggaatgc gtcgccatag cgcacgataa
     2941 ggagaaaacg gaagatcgga cagacaaaag gcggccgcga agagaccggc gatgagaatc
     3001 caaagccctg ttgtgaagct tccacgcaca gtccatatag ccaataccgc aacgagccag
     3061 gagacgggat tgacccatgg gcttacaacc ctcagcatcc agcgatcccg cttttcgagc
     3121 ggccaaaaaa ccaggcgcga ggccgggatc ttacgcagag gatcggtgct gagcggaaac
     3181 aacagcacca gtgcgatcag cagatataag aagcctcccg cgttctgcaa cagaagagcg
     3241 gtaaccagga aaaaattgtt gcccgccacg gatcccaggg atttctgatc gcggaagaag
     3301 gcccgcgtga gtgcccttaa gacggcgaag acccgagcca ttgcagttcc tccaccgccg
     3361 gcgcctcaac cagatcgaaa tagtgttgct ccagggactg cggcagttct tcggccggcg
     3421 agttccatac aatgcggcca ttacggatca ggatgaactg cgtggcgatc tgctcggtca
     3481 ccgacaggat gtgcgaagtc aggaacaccg tgacaccgcg ccgggcgacg tattggagca
     3541 ggtcccgcat gattttggaa gtgacgggat cgatagcttc aaacggctca tcgaggaaca
     3601 gcacgcgggg attgggaagc agcgccatgg caaaggcggt tttcttgcgc atgccgtgcg
     3661 agcaggatga agcgaaggta tcgcggcctt cttccagact caaggcgtgc agcaactggc
     3721 tggtgcgggc acgggtctcg cgccggtcga gcccgtaaac atcgcctgtc agaaggagat
     3781 gctcttccac cgtcaggtca tcaaagggac cgaggccttc gggcagaact ccgatgcgtt
     3841 gcttgagttc gcgcgccgct gacttcacgt cgagcccaca cacccggatc tcaccggaag
     3901 aaggggccaa cagccccgta agcattttta tggtggtcga tttccccgcg ccgttgggtc
     3961 ccaggaaagc acagatcgtt ccgggagcga tatcgaacgt caagccgtca acggctgtga
     4021 aattcccgaa gcggcgcgtt acgttgtggc aacggatcat tacgttattg gagccgcgcg
     4081 ggaagcggaa tgcccgcgcc ttggggagcg atatccggaa tggtgacggg gagatggccc
     4141 tcaatgggaa tctctgcctt gatggctttc aacgccgcga cttccgacgt gggcgccgta
     4201 ctgtatgtgt tcaggtatgc cgccacatcc ggaaaactcg acagcagata ggggttcccc
     4261 agcgagacca taacgaccgg agcgggaccg gctatcaact gttcgacgaa tttcggatac
     4321 ccgccggcaa gcgctacatt tccacgatag gcggccacgg aaacaaaggc atagaggacc
     4381 acggctttgc aggccgccgc ttttttggca gcctttgaaa gttcggcggc tggagtggcc
     4441 ggcgtaaagc gatgccagga cgcgccaggc agttccttcg gcatcaactc tgcgagcttc
     4501 cgtccgctct gtcccagccg gctctcggag agaataaagt gacaggccga agcaggattt
     4561 tcgagcggca ggatatcgtc ctgattcttg accagcgtta tcgcgcggcc ggcaacctgc
     4621 tgcgcgaggt cctgcgactc cggcgaattg acaacgcggg agatgttctt taaatcaacc
     4681 aagctgtttt cgccgagccc tacgcgaagt ttggcggcca gcacacgctc caggctctcc
     4741 tcgatacgct gccggctgat acggccgctg ttgaccgcct tgagcacagc cgcgaccgcc
     4801 acgtccgggt cgggcggcat cagcagcacg tccgctccgg cttctattgc acgcaccgcc
     4861 gcttcgccgc ccggtaagat cttcgtcaga ccctgcatgt ccatggcgtc cgtcacaacg
     4921 atgccctgaa agccgagttc ctttcgaaga acgccggtga gaatggcgtg cgaaaccgtg
     4981 gcgggagtat ccgaggggtc cagcgccggc accgacatgt gagcggtcat aatggcatcc
     5041 actccggcat cgatagcggc ccggaacggt gtgaactcca cttcctccat ccgggtcctg
     5101 gccatttcca gcctcggcag acccacatga ctgtctaccg ccgtgtcgcc gtgccccggg
     5161 aagtgcttgg cggtcaccag aacgggatgc acggcggatc gcgcaccttc gatgtaggag
     5221 cgaacgtgcg atgccacttc ctgtgggttc tcgccgtagg atcgaatgct gatgatggga
     5281 ttggcggggt tgttgttgac gtcggcatcg ggcgcgaaca cccaatgcac tccgagagcg
     5341 cgcgcctcct ccgcggtata ggcgccttgc gcgtggctgg cttcataatc gcgcgccgcg
     5401 ccaaaggcca tattgtgagg ccacttggtg gttcccgata cgcgcatgga ggcgccgcgt
     5461 tcaaaatcac cggctacgat cagcggcacc ttcgtcactt tctgcatccg gttgatgaac
     5521 gaagccattt cgtggggctc ggcgttcttt gcgatgccgt tctggacgcg gttcaccaga
     5581 atcaagccgc cgacacggct ctccgcgacc agatgcagaa aacgtttgtg ctccgccgat
     5641 ttcgggccgg gcaagtcccc gtagaagggg atgacgacca actgcgcgat ccgttcacgg
     5701 agcgtcatac cactccgcca gcgctccagg tgagccggag tttgcgcttg cgtttctgcg
     5761 ctgctgccgg aatagctgaa agcggccagc gcggcaatgg tagccaccgc aagcttcgag
     5821 tggatcgtca acctagtgtc ctgtcatccg ttcgatgcgc acaaccgctc cgtgaccgtc
     5881 gcggctcaga aaccttaccg agccgcgcgc gcgagcaagc ggtcgtgact tttgaagcga
     5941 acttctgtga cagcacacaa gcacttattg tacgccgtgc ctttttgccc tgagactgaa
     6001 ttgctgccgg cgcaccggtt tcttcggcgc cgaaagaagc cgcttcaccg agcgcacaag
     6061 atgaatcact tcattcgcgc tcttctgtat gaccacgtcc gcacccgtat tgctttcgtt
     6121 caggcccaga gtgtccgcaa acccggagat cagaatcacc ggcatcgctg gatgttcccg
     6181 acgaatctca cgaatcaatt ccactccgtt catgtcaggc atcttgtaat ccgtgaccac
     6241 cagcgagaac gtcttgcggg cgaaagcatg tagcgcttct tccgctccct tcgcgatgga
     6301 aaccgtgtag ccgagttcct ccaaaacgga tctgcgcgcg gccagtccgt gcctattagc
     6361 attgacgatg agaatatgag cgggaggagg agttaaagaa atatcagcat tcatcgaaga
     6421 ggcgaactta ccagagccac cggcgctgtg tcaatcgcgt ttctaatcgg ctgcaaaacg
     6481 gtaagatctt ccgcttgaca tccgcaagcg ttgtcttatt cgacttcgcg cggtgctgtg
     6541 tatcccgctt tggcgatgat tttatacttg atcgatttgc ccttctggtc ggtcactctc
     6601 aacgcttcgt tggtggcggg attgaccagc tcgactttga tcttgcgata tttgccatcg
     6661 cgcgcctgat tcgttggctg gtacgccagt gagtactggt tgcgcagcgc ctgggcgatg
     6721 gacccgaaga tgctcgggaa ctctccgtag aaacgtggga aatatgcctg tccgcccgtc
     6781 tcattggcga aggtgcggag ctggttgtcc gcttgcagga agtccagccg cgcgatagaa
     6841 cccagatagc ctcgcgcatc gtaccattcc cgcagcgcct gcatcaggcc gatcgcatag
     6901 atggggacac ccgccgtttg caacgctttt cgcgtcttat cgaatgtcag cttgctgaac
     6961 gtgtcgatac cggaggaaat taacacgatc gccttcctgc cttcgatctc ggacatgcgg
     7021 tccgccgtat cggtgatggc gtcgaacagg ttcgattccg aataggcggc gatccggagg
     7081 cgctgcatcg cgtcataagc ctgccgctta tcggtcgaaa aatcggagag gatttccggc
     7141 cgcatatcgt aggccacgac agcaacgtag tcctccggct tgagggtttc gagaaaacca
     7201 tatgccgcgg tcagcgtctg gtaccacgtt tccccccaat aattctggaa caggttgctg
     7261 aactcgatga ccagggcgac ggtcatgggg gcttcaccca tgctgaagtt cgtaacctgc
     7321 tgcggaacgc cgtcctcgag gacgcggaaa ttgccacgtg gaatctgagg aatgaaattc
     7381 cccctgttat ccatcacggc gacatcaagg tctacggtca gaacgtcgct cttgaaactt
     7441 gccgtcggag cgacatcttc cttgtccttc ttgaactcgg atgggatttt ttcctgctgc
     7501 ttcgcttctt cgggtttgga ggtccgcggc cgtgctaccg tttccgagga ctccttcctt
     7561 ggaccttcct gggcggacaa aactaccgtt ccggcgagca gaagcaacgc cggcatcagg
     7621 caataggatc ttgcaaatct catctatacc ccctaagccg taaacgcgta atccggcgca
     7681 atctttactc catacaacag tgctgacgtg ccgccgcccg gcaccgttac ctcagatcag
     7741 ttcacccatg ggccccggtt cgaatcgcgc ttgcgcatat acacgtcgaa ttttctcttc
     7801 gctctctgca tcttccagcg attatattgg cgaaacagat cgccggcaaa gttggtacgc
     7861 actcgcgcgc cccggcccca cttcaaatag ccgtatccga agatcattcc gccgaggtga
     7921 gcgacgtgac tgacgccgct tcccgtcgag cccagcgtca gcatgaactg aatggcgccc
     7981 atgatcatca cgaagtactt ggctttaatg gggaagagga accagaacag cacggtgctg
     8041 ttggggaaca acattccgaa cgcaagaagc agaccgaata tcgctcccga cgcaccgatc
     8101 gtgcgggttt cgagggtacc gaataacagg ttgccgatga caatgcagat cccggccccc
     8161 accccgcaga tgaagtaata ccgcagaaag cctctcgtgc cccacgtacg ttccagatcc
     8221 gtcccaaaca tccataacat cagcatgttg aacagaatgt gcgtgaaccc gtacggatcg
     8281 tgcagaaaca tgtacgtgaa aagctgccag atgccgaaga gtttgagtac cgcgacgggg
     8341 acgagcccaa atggatagaa aataccgccc aggccggtgc gaaccgcaaa gaaatacaga
     8401 acaaataatg ccgtattaac gataagcagc catttcacgc ccggcgggaa cctgccgccg
     8461 agggaggcaa aaccaccata gccttgatag gtctgtctca tgaaaagggg tggggctagc
     8521 cctttctgac agaataacac tgcgcgcgcc cgtgatagcg cacagtggta tgctaatcgg
     8581 aaatcacctt ggaggaggct ttgaccatgc aatcgaggcg tacttttctg gcgggctcct
     8641 tactagccta cgcgggcaca cggccgttgc gcggtgccgc gcgcagctat gccgaaatcg
     8701 aaaagttgct ggcccgtgac gatatcaagg gcaagctgag caaatccgag ctgccgaccc
     8761 ctgccctgat tgtcgacctg gacgcgtttg agcacaacgt cgccagcatg tccgctcatg
     8821 cgaaagccag cggccgcgcc ctgcggcccc acgcgaagac gcacaagtgc cccgagatcg
     8881 ccaagatcct catcaaggcc ggcgccgtgg gagcctgcgc cgcaaaaatc agcgaagcgg
     8941 aggccttggc cagggatggc gtaacgggtc ttctggttac gaccgcggtg atcgggccgc
     9001 ataaaatcga gcgcgccgtg cgcctgacct cccgccggcc ggagaccatc cttagcgtgg
     9061 acaacgcgca gaacgtgcgc gacctgaatg acgccgccgg agccgcgcga caaaagttaa
     9121 atctcgccgt ggacctttgg atttccaacc ggaccggcat ccgtcccgga gaaccggcgc
     9181 tggccctggc gcagttgatt gattctctac cccatgtaaa gctggcaggg attcaagcgt
     9241 acgccggaaa cgcctcccat accaagggat ttgaacagcg taagaagttt tcgcgcgagg
     9301 tcatggcgca ggccgtggat acccggcgcc tgtttgagag ccacggaatc caatgcccgc
     9361 tgttgaccgg cggttctacc ggcacttaca acatcgattc cgaaatcgaa ggcatcacgg
     9421 agcttcaacc cggcagcttc atcttcatgg atatcgatta caaccgcatc ggcggtaagg
     9481 acggcgacgt gtaccaggat ttccggaact cgctgttcgt taccgctacc gtggtgagta
     9541 agccaacgga ttcctccgcc atcgtcgatg gcggcctgaa ggcgttcgcc acggaccgcg
     9601 gttacgggcc gtcgctgcgc aaaggcgaag ggacaccgta tgcgttcgcc ggcgatgagc
     9661 atggaaggct gagcttagcc gattcagggt cctccgtgaa cgtgggcgac cggctcgatt
     9721 tcgtcgtgcc gcactgcgat ccgaccgtaa atctctacga tcgcatctat ggtgtgcgcg
     9781 gcgacgcggt cgaatgcgcc tggcgcatca ccgcccgcgg catgagccag taatctggtt
     9841 cacgttgcgc agccgcgtcc catgcccacg taggtaagcc ccgcctggcg aacttccgag
     9901 ccgtccagaa tgttgcgggc atcgaagacg atacgcccgc tcaggcgctc atgcaattca
     9961 tggtagtcgg gactcttgta ggtatcccat tccgtcataa tgacgagggc ggcagcgccg
    10021 tccacagcat cgtacttatt tccgcacaag gtgatgcggc tgccgaaacg ctccgccgcg
    10081 cgctggcccg cctcggggtc gtgcgcccga atccgtgcgc cgcgctccag cagatactcg
    10141 atggcgtcca cggccggcga ctcccggatg tcgtcggtcc gcgctttgaa agcgattccc
    10201 cacaccgcga gagtcctgcc cgccagcggt gcgagttgcc gttcgatgcg ccgcggcaga
    10261 tagatcttgt gccggagatt cgattcttcg atggcgggaa ccaggctcaa gtcggccccg
    10321 tgctcgcgcg agctctcgcg cagcgcccgc aggtctttgc cgaaacaact gccgccgtag
    10381 cccgggccag ggtacagaaa aaggtgtccg atgcgggggt cgctggccag tccggtgcgc
    10441 acgtgctcga tatccgccga cacggcatcg cagtatcgcg cgagttcgtt aattaaacct
    10501 acacggcatg ccagaaaact gttcgccgcc agtttggtca cgatggccga tttgggatcg
    10561 gtaagctgta gcacaccggg tttacgtttc gtgaaggggc ggtacaggcc ctccatgaac
    10621 tggcgggcgg cgggcgaagt ggcgcccaca ataatgcgat ggggctggct gaaatcgcgg
    10681 atggcggttc cttcggcaag aaactcggga ttggagacga cttcgatctg cgcaccgctc
    10741 ccctccgttt cgccggcgag gatcgtctgc acccgggtgt agatgtccgg cgtaaccgtg
    10801 cttttcagaa ccacgatgtg acgcttcggc cggcgcagcg cccgcccgat ttcgtgcgcg
    10861 gccgcctcga catactgaaa atcgtaggcg ccgctttcaa gcgatggtgt acctaccgcg
    10921 ataaacgtag cgtcggcgcc ggccacagcc tccgccgttt ctgtcgtgaa ggacagagtg
    10981 ccgtccgcga tcgtgctctg aagaatctcg tccagccccg gctcatgaat cgtactcctg
    11041 cccgcgcgga gggcctccac gatggaggga tccttgtcga cacaagtaac ccggttcccc
    11101 agagacgcga acatcgcacc gctcaccagc cccacataac ccgttccaac cacaaccagg
    11161 cgcttcatac gagtagtacg gtagcacagg aggcgcgcgg gccggcagaa acaaaccgcc
    11221 gagtgggtct agtacgggtt caggccgcct tgtacatttc tttccccgac acccacgttt
    11281 tttccacttc gatacgtttc gaatcggggt caaaccggaa ctgcacgacg tccccgcgct
    11341 cgcccggggt aaggccccgc tgccgtgcgg cgattctggc cgcgcgggcc gggttccgcg
    11401 tagccatcgt aatggcttct gtgaggctca ggcccgccag tttcatgagg ttctcaatac
    11461 cccgctccat gcgcagcgcg gacccggcga gcctggtgtt ttctacgagc gtgacacgat
    11521 cgtccgggtg cagctccact tccaccccac caagtttgta gcggccgggg gcgcatccgg
    11581 cgggcatgac ggcatccgtc acaaggaagg cgcgttcgac ccctttggca cgcaacccga
    11641 ccttgagaaa ggccgccccg aggtgaattc catcgacgat aaagcccgcg gtcagccggt
    11701 cttcggccag ttgttcccag atatagttgg ggtgccgctg caataccgca tgggcgccgt
    11761 tgccgatgtg cgttgacagg gtcgcccccg cgctgacggc gtcggcgatt tcccgcggtc
    11821 cggctttcgt gtgcccgatg ctgacgacga ctccctcgcg caccagggct tcgatatagc
    11881 ccaccgcttc aggccactcg ggcgataacg tcaccaggcg cacatttccc cgggcggcgt
    11941 cctgccaccg gcggaactcg tcgagatcgg gttttcgtac ccaggccttc ggatgagcgc
    12001 cgcgcggtcc gtcttccggc gagatatgcg gcccttcgac gtgaaagccc tccatcgccg
    12061 gtccttcctc caggctctcc ctggctcggg ccaggttgcc gagggcgcct gccatgtcgt
    12121 ccggagcacc ggtgattacg gtcggaagaa accgcgtaat gccggtggaa tgcagaacgc
    12181 gaatcgaccg cgcgatctcc tcatgcggag tggatggggt gttgtaatcc accccggcga
    12241 agccattcac ttgaaggtca atccaaccgg cggaaacgta ggaatgcgtg ccgccgtttc
    12301 cgatcacgtg atcgaccgac tgaatcaccc ggtcgaaaga aatctcaata acggcgccgg
    12361 tcgtactatc tatacctcta caattttcag gcattgtgat ttttgcacaa ctttctcaca
    12421 ggcttacgcg gctaaacacc agagaaatcg agaataaaaa gctgttggaa actgaaataa
    12481 acaagcgatt tcgactggaa ctatcgcgtg cgccttgata gaactacagt aagatcctaa
    12541 gcaggtgcga ccccggtgaa tatctcgggg gaggtggttg ccgggtcgca tctgcgggtt
    12601 ccattcagtt cgccgctacc ggctgtcccg cattcacctt tgtaagccat ccataggaat
    12661 ccggtttctt tccgttgatg acctcgaaga attcctgctg aagcttttcg gtgacgggcc
    12721 cccgtttgcc ggatccgata acgatgcgat caatggaacg tatgggggtg atttcggccg
    12781 cggtgccggt gaagaatacc tcatcggcaa tgtaaagcat ttcccgcggg acgatggttt
    12841 cgatgaccgg caggcccaga tcttcggcca gcttgatgac gctatcgcgc gtgatgccgg
    12901 gcaataccga agcgccgagc ggcggcgtca ggatcctgcc gtcgcgcaca acgaagatat
    12961 tctctcccga gccttcgctg acataccccg cctcgtcgag agcgatgcct tccgagtagc
    13021 cgttgatctg cgcttccatg cggatcagtt gcgagttcat gtagttggcc cccgccttgg
    13081 ccagcgcggg aagcgtgttt ggagccaggc gattccaact ggagatacag acgtccacac
    13141 cttgcgccag cgcttcttcg cccagatact tgccccattc ccagcaggcg atgtagatct
    13201 cgagggggtt attcccggca tgcacgccta cttcgccata accgcgaagc acgatcggcc
    13261 ggatgtagca ggcatcgagc ttgttgacgc gtacgagctc caccatggca tccaccaatt
    13321 gcccggcgtt gtagccgagg ttgtccatcc ggtaaatctt ggcggaatcg atcatccgcc
    13381 gcacatgttc ccgtacccgg aaaatagcgg gacctgatgg tgtcgaataa caacggatgc
    13441 cctcaaacac cgagctgccg tagcttacga catgagacaa cacgtggatt tttgcatcat
    13501 tccagccgat gaaccggccg ttatgccata tgtattcagt aggcgtcaac atgctgatta
    13561 ttatatcgaa aggcagtttg tgaaacgatt ggaaatgcac gtgcatctag catggctgga
    13621 gggtgtcact tgaaacgacg gctgttttcg ttagttcttc tttcgagcct ggtctgtccg
    13681 gcgctcctcg acgcccgcga cctgaagccg gggtggaacc ttttttcgaa ggaacaggat
    13741 gtgcagcttg ggaaggaagc ggcagcccag gttcgccagc aaatgcctat cgtcaaggag
    13801 gcggacatca acgactacat ccggcgaatc ggccagcggc tggcttcaca acccgaggcg
    13861 ggagattttc cctactcttt cgaagtggta gcggataaga gcatcaatgc cttcgcgctg
    13921 ccgggcgggc cggcgttcgt gcatacgggc ttgatcctgg ccgccgaaaa cgaggcgcaa
    13981 cttgcgggtg tactggccca tgagatcgcg cacgtggccc tgcggcacgg caccaatcag
    14041 gtgtcgaaag cgaacgccgt gcagattccg gcaatgattc tgagcggcgt gctggggcgc
    14101 ggcggatcga tgatggacca gttaacacaa cttggaatcg gtatcggagc gaactcggtg
    14161 atgatgaagt tttcccgtag cgcggaacgc gatgcggacc ttctgggaac ccgtatcatg
    14221 tccggcgccg gttacaaccc gatcgagatg gcccgcttct ttgaaaagct ggaagccgaa
    14281 ggcggcagcc gcggccctga atttctgtcg agccatccga accccggaaa ccgcgtgaaa
    14341 gcggtgcagg ccgagatcca gacacttccc agaaaacaat acaccacgga caccggcgac
    14401 ctttcgcgca tccagagcag cgtatcgaag attcgtgtgg aacccgcgcg cacaacgccc
    14461 gcacgctcca cgcctttgcg gtcccgtcag cgctcccgtt gaaggactat tggaactgca
    14521 ccggcgtcga taggacggcg gttgcatgtc ttgcgaaaaa accacggatg tcggccgtgt
    14581 agtctttcaa gatagcctcg ccgagcccct tgtaatcgcc acaacgatag agcgcccgag
    14641 caatggtgat ctcgcgcata gggcccagcc ttcgcctgcg gtccatgtcc gcgtcatgca
    14701 ggggttccag ttccttcatc acgtgaccgc gcatcccggg ttttgcaagc aatcgcgcca
    14761 gaggctccgc cgcggccgga tccgccagtt gctcaagggc aatggcgacg gcgcgatggt
    14821 gcgacagcgc cacggaagca tccagcgttt cgagcttgcg cagcagtgct ggtacggcct
    14881 tccggtctcc cgttcgcccc aggcccagaa tcagcgtgtc gatgggggtc ggcaaatggg
    14941 cgtattcggc cattcgtcct tgcagaatcc tggggtccca ttcctggatg gcatccagag
    15001 cttccatgag taccggtgcg acttcgggga cgcctaaaaa agccagcaga ttcgcataag
    15061 tgagcttgtg ccgcgggcgc gccgtctcga acgttttccg aagcaggggc agagcggcct
    15121 ctttgtgcgt aagaatcacc gcgagccaga cgcccgcttc gtccgggttg gttgcaaagg
    15181 cgacgttctg aaccgcctgg cgaatctcgg aatccggcag cgggaacgaa tcctgatgtt
    15241 cgaggaccgc ggccggcagg ctgccgatcc cgacgagatg gcgctgaagc gaacgtacat
    15301 cgaggtcgcg aagagggcgg ccggaagcgg ctgtcatcgc ggcggcgacg ccggccgcat
    15361 agccctggtt ggccatgtcg cgctgcatac gaaccattgc ggacgcgtcg cgatgcatgc
    15421 tgatgccgag tccggccacg agaatgcctt ccagcccgcg agggagtaaa cagcggtacg
    15481 gggtgtagca ggcgccgccg ggagcgggat gattagcgag ccgggaacgt tgatcgtgcg
    15541 gcagcagggc aaagagatcc tcactcgggt agccgtggga atcgtaatcg ctggcggaat
    15601 atccgatgct gtccgggtag gtgcgcccgg ctatctggtc gaggtaggaa aggatgtgct
    15661 ccccgacgac ccggcggcgt tcacgagtct gaatgagcgc gccggagtcg taggcatcct
    15721 ccgtcatggt ctttctggcg ccgacgagag ccctccagac atcgagcatg tcgttctcat
    15781 cgacgagcag gtagtccgtg ttgacgtagc tgctggcggg cttacgggta gggagtcccg
    15841 caccctgcat cgccagatcc tcggcgtcgg agccgaacat ggccttggca ccggcggaca
    15901 cggcgacatc ggcgttgccg gtggcatcga tgaccacctt ggccagcacg gcgccgcgtt
    15961 gctgcggagt gacgatgacg gctccgcgca cgcgatttcc ctccatgatc gcgcccgagc
    16021 cgaggacgcc gaacagaatc tcgccgccgg tcttgcggat ctctttgcgg taccactcca
    16081 tcttgtcatc gatggtgaag gtctcactgg ggaagggaac ctctttggcg aagccttcgt
    16141 tccggccgtg ataaggcttg ccgatcatgc cgagggtacc ggttcctccg agtccctcct
    16201 gaaactccgc gaccaggacc ttcgctcccc ggcggcccgc gccgatagcg gccgcggctc
    16261 cggacgtacc gccgccgatg atgaccacat cgtattcccc caggatgggt aatgctcgcg
    16321 cgggggaatt gacataaccc gtgcctggct ccgtgggacg caggccggag agaacttcgc
    16381 ggatctctcc ggccgaagta ccagtgcgca gcacggagct tgcaacctga ggagccttgg
    16441 gactggacag gcttgcggcc tgacgggcgg cgtccgcgcc gatgaaccgg ccgaccgctt
    16501 cggcaaccgt cggacgcatc agcagttccg ccgcgtcgcg gtcaagatcc gcgcggctgc
    16561 ctgcgacgta gagccggtcg atgccccgtg gctgaaaatg cccgatgtgc gggcgggtga
    16621 aaccctgcca ggcggaggcg ggacggcgcc cgataatccc attcgggtaa acaaaatgca
    16681 gacgggctga cgcacgcatc tgtcccggac ggtacgtcaa gtcgcgtgct tcctgttcga
    16741 cgcgtccgaa agacagggag ccggtatcgg gcaggttgag acggagcgag tactccagat
    16801 agtcctgatt ctgatccggc atcggaaccc gccggcggat ggcatcttcg ttgaccggct
    16861 tgcctcccaa cacgactcgt tggaactcga cggttccgcc cgcccaggga tggaatttcg
    16921 cgcccgcctg ttcggctacg gaagcatggg ctgtagcgtc gacgataact ttggcgatga
    16981 cggcctggcg tccagcgcgg ttggccatga caaggccggc aggttggcct ttcccgtcaa
    17041 gcagaatctc cgtcgcggtg cagcccagca gaaagcgaac gccggctttg gtgagggcgt
    17101 cctcgagggt cttcttaaga cgcagcggcg gagccgggcc tggcttgctg aagatcgttt
    17161 ccgtgagtgc tccgaccggc ttctcccctt cttccagcca gaggcgcatc gtgtcggcaa
    17221 ggtcttcacc gaggtagagc cggggagcga tcagaagaac cgaccggcct tgggcggcgg
    17281 cctcaagagc ggcggaaacg ccggcaatac cgccaccggc gaccacaaca tcgacggtgt
    17341 cggctaccgg gacgcggcgg ggggattcga cgatcgccgc gggagacgtc gtgccctgtg
    17401 ccagcaaagc cgctgccaga gataacaagc aaaagcggtg agtcatgtca gccctaaagc
    17461 tcagaataca agcttgagtc cgagttgcat gatgcgcgca tcccgtgcgg acgtgatgac
    17521 gccaaaattg ccgctgatat tcgtgccgac cgttccgggt tggaaagtcg cgttcgggaa
    17581 tcggaggttg acctgattga aaacattgaa ggcctccgcc ctgaattgca ggcggacacg
    17641 ctcccggacg taggtatcct tcatgagcga gaagtcgaaa ttcaccaggc ccggcgagcg
    17701 cacatccggc aggacgcgtc caacgttgcc gaaagtgaag ttgggcggat tgacgaactg
    17761 agtggtgtca aaccagcgtg cggcggtggg gttgtccagt ttcgcgctgg cgcccgttga
    17821 attcggccgg tctgccagga aattattggc gccccgcacc aggatcggca aaccgctttg
    17881 catggtgctg atgatgttcg cctgccagcc acgcgcaaat ccgtccagaa caccgtgaga
    17941 gaaactccag tgcttacccc ggccgaacgg aagttcgtac agaccactga tcacgagacg
    18001 ttgggagaca tcggtcggat cgagcgacca ttccgcggcg cgattgaact tgccgttctg
    18061 gtaattcgtg acgccgccct gctcggaacc gatggaggtc gtctggatgc tgtcgctgag
    18121 caacttgcca ttggtgtagg acagcagaat gctgaagccg ttggacagac gccgctccac
    18181 actgagaagc atggcgttat agatggaact tcccagagtg ggcctgaaga ccgccacctg
    18241 gttgtagtag ggaagcggac gcagagactg caggcgggtg atggtggcat tccccagcga
    18301 tcccggcacg atgccggcgt agggattggc cacctggttt tgcagcgcca agccgagggc
    18361 gagatgttga ggatcgagct gattcaggtc atagttgccc gcggccatgt gactgacacg
    18421 gttcgcgctg tagctgatct cgaaccgcca gcccttcagt ctctgttgca gcgagaggcc
    18481 ccattgttgc gacatgggaa cctgggcgtt ggattcctca taggtgacgt tctgaccgag
    18541 gaatgcgctg ggacccaacg cggcgcccag cggcgcgatg gggggcgacg gcaacccgtc
    18601 actgaactga aacgccggaa agttcgtatt gttgccgggc ggttgatagt cggtggtggc
    18661 cgtcgcaaag cctgctgtgt tgccatagaa gctgttgttc acgagttgcg ggtagaagat
    18721 gctgtatccg ccgcgcaaca cggtggcgcc ccggccggta atatcgagtg cgaatcccaa
    18781 gcgcggtccc cagttgttgt agtccggctc gcgcacgctt cgtccgaagt caacgccggc
    18841 gtactccatg cgcccgatga ggttgttctc gggattcaga gccaacgggt tgaaattgga
    18901 gatgccgtcg tagcggtcaa tcggccaggt ctgatagtcg tagcgcaggc cgaggctgag
    18961 cgagagccgg cgtgtgacct tccagtcgtc ctggatgaac ccgctgatgg aagaaccgag
    19021 gttgctcgcc ccgagatagc gcgtcagact ggcgctgctg accgagcccg taaggaaggt
    19081 ggcgaagccg aagccggtgc caccaggctg ttgagggtta ccggtcaatc cggcggcaaa
    19141 agcgtacgat cccgagggat tcgtgttgtt aagattgttg gcctgaagct ttcgccactc
    19201 cccaccggcc ttgagcgtat ggttgccccg ggtcagcgtc accatgtcgt gaacctggaa
    19261 gatgaccgtt ccccgcaagc cggcgtaaaa cgtgccgaag cttgtcattc cgttgctgat
    19321 actcggaaaa gtgtcgggag gaacgctgga gggcaatccc agttgctgtg gaatgctctt
    19381 cccgtagctt aaggactcaa aggggaaata ggaccgcgcc aagccggccc ggaattcgtt
    19441 gattgtcgcg ggattgatgg tatgggtgtt gttgatgatg aggttgtgag taacaaagtt
    19501 gtcaatgcgc tgccggacga ggggatcggg ataaggcgag ccgccgacgc cgccgtcggt
    19561 acggtgacgg tagtaagtgt agcgcccata gatggagttg ctctccgaaa tccggtgatc
    19621 cagcttggaa gtaagctgcc gcatggactg cgtctcatcg atcgtgctca ggtagttatt
    19681 cgcatttgta aacggatcgc tgggagcccg gtttggcaga ggataaaagg gcagaatgtt
    19741 ccgcgagaca gggtcgatac ggttctgcgg gatccggttg ccggcgaaag gatcccggat
    19801 gaagccgctt ccggaggggt tggcccttgt agtcagcgga tcgaagatcg gaatcagggc
    19861 gccgctggcg gcgcgcaagt tggagaagtc gccttcacgt tccgcggcgg taggcacgcg
    19921 gatgatgggg ttctcataac ggcgatagcg ccattcctcg taattcgtaa aaaagaagct
    19981 gcgattcatc cccgagtaga tgcgtggcag cacaatcggc ccgcccaagg atccgccgaa
    20041 ctggttgtag cggaatggcg cgcgagtgtc ggcaaacgta ttgcgggcat cgaatttgtc
    20101 gttgcggagg aagtggtaca gcacaccgtg tggatcgttc gttccgctct tggtgaccac
    20161 gttcaccacc ccgcccagag tgaagccgta ttcggccgac atgctgttat gctgcacctt
    20221 gaactcttcg acggcatcca cggccggttg cacgttgatg tcagcctgct gcgcctgatt
    20281 gttgttgccg ccgtcgagca catagttgtt gaatacgccg ggacctccgt tgatgctgac
    20341 cgccgagagc gccgtgccgc ggttggcaaa gccgctgttg gtgggtccgg cctgggagat
    20401 gacggccgga gtcagcatga cgagggccag cgcgttccgg ccattcaacg gcaaatcgac
    20461 aacgcggcga ttctcaacca ccacgccgag cgtggcgttg gaggaatcga ccatcgtggc
    20521 gtcggacaca acctcaatca actcggtcac ctggccgact tccatctgga tgtcgatcct
    20581 cgcaacctga tctacaacca ggttgatacc atcgcgactg agaggcttga atcccgacgc
    20641 ctgggcggtg atgcgatagg gacccggctg aaggagcggc accgaataat tgcccgattc
    20701 gttggaaacg gcgcgccgcg acacgcccgt cgatgtgttc gtgaccgtaa tctcgactcc
    20761 gctcatcacg gcgtcactgg tgtcggtgac gatccctgtg atgcgggcgg attggcccca
    20821 tgccggaact gcgcagagta gagccatcca tatcagcgtg gtattgcgtc tcatgattcc
    20881 cgtctcctta tccggttacc tgcctgtcaa tccgaaatcc ttcaatctgt cagggtccca
    20941 acccgccctg gcgataacgt ccatcgttct gtacaactcg agcgactgct gatggagctc
    21001 acgcaattct ctccaggcga tcccggcatc cacgccgccc aggtcaagcc cggcaaacag
    21061 cccgccgatg aggtagtcca ttcgggccgt ttgccgctcc agcagaacac cggactgctc
    21121 ccagtgcccc ataaccgggg ccggttccat ggccgcggtt gaagcgtcgt agtcaagctg
    21181 atccagcagt tgaccaagtt gtgtaaggcc atgccgcaac tcttgcaggt gttcttcgac
    21241 gaggcgtaaa aaacgcaccc ggtcttccgg cttcattgcc tcaatccggt gggcggtgaa
    21301 gaccgttgcg agggttcgca aagcttgcgc ttcctgatgc gcaattccgg caagccggac
    21361 cgtctggctc gagtaatcgg ccatgtcctt ccccgcgcgt tccggcggcg agagacgccg
    21421 gaagtgttcc ttcaaacggt cggccagcaa aggcggccgg ggctcgatca gaagggtggg
    21481 tgccgcatcg gcagctacgg gtgaaaggtc ttcggacggg atggccaccc gcacgtccgc
    21541 cagtttgaca ccggccagag ccgccctgag attttcggcc tgttgcaggc gatcggcaac
    21601 gccggtaatg cgaatcgaat cgcccggcac gcgcaaaact tcgatcgtgt tgccccggca
    21661 caatccggct tcatgcaaag cgagataagc cgcgaattcg gcggcatcaa gctccgcgga
    21721 agttggaggt acgaacagca gcggcggttg cgttttcggg atgcgcggca ccgtcacggc
    21781 cgccaacggc gcggcttccg gtggagcggg ggccggtgca gcagtgggcg gtggtgttct
    21841 cacggctgaa ggacggggtg ccggctgtct tgcctccggg acggtggagc gaaaaacata
    21901 gtcgcgaact ggaggccatg cacctacgcc cgccagagca gccgcagccg cggcgagagg
    21961 ccagaacaca cggcgggcca gaaagaaaat ccgaaacccg gattgcgggg actccggttt
    22021 aagcagcgcg gcgtggcgtg caagacgcgc cgaaaagcca ttcgagctgt gcggcgtccc
    22081 ctccagggtc cggaactgat cccgccgctc ttccatgaag gcgtggatcg cgctgtccag
    22141 gcgctccagc agagtgcggc actcccaaca gtcccgaaga tggcgcgcag ccgcgtcgtc
    22201 ttctttcctg gagagggcgc catcgatgtg ttgaagaaga atctcctctg ttaagtgctt
    22261 ctccgctccg agggctgacc accaattctt agccatggat ctgcctcgcg atcttctgca
    22321 ccgccctgga taaacaggta gtcacggtgg aaacctgcag tcccattaac ccggcgatct
    22381 cccggtacag aaggccttcc gccctcaatt cgagaacctg gcgctcctgc gggctcagca
    22441 ggtagagccc atcacgaatc tgccggtggc gctcggcggc caacatcgcg gcttcagcgc
    22501 tgggcgctgt gtccggatac tccgtgtccc ttccctcttc gtggacccat tcttcctgcc
    22561 cgcgccggcg cagaaaatca atcgccagat tgtgtcccac ctggaaaatc cagggccgca
    22621 gccgctttac tttccggttc ttaagaacct cttcgtagag gcgcagaaag gtctcctgga
    22681 cgaaatcctc agcatgcccg gggtcgccgg tgaggcgcag cagataccgg cggagcccgg
    22741 gaccgagctt ctcgtattgc gcacagaccc actcctcgac gagatgggaa tcggagcgct
    22801 tcgacaagag atagtccgat cctgtcagcg tgtcatccat ggaactgctt ttcgtctgta
    22861 ctcaccagga cgaaagacgg cgggcttttt actacagaac ttcaaactca ggcaaaaagg
    22921 cgcgtcatcg tacactggac ataatgtccg cctatgatgc cattcttttt gatttcgatg
    22981 gcgtgctggt tgatagcgaa cccgtgcact tcacctgctg gcgcgatgtg acggcgccgt
    23041 tcggtgtgaa gctggattgg gattcttatt tgaaggaact ggtggggatc accgaccgcg
    23101 aagccgtgga atggatggct tcccacagcg atcctccggt tgacccggaa aagctatggg
    23161 cgctataccc ggctaaaaag gcgatgttcc gcgaaaggat gctggccgcg ccgccgttcg
    23221 cccccggcct cgctgaattt ctcgagggcg cggactctta caaattgggg gtggtatcgt
    23281 cgagcgccag aagtgaagtg gagccgctgc tcgaagccgg cgggctgcgg cggtatttcg
    23341 ataccgtcgt ctgcggggaa gatgtcaccc gtcataagcc tgcgccggag ccgtatctgc
    23401 tggcagccgg gagagtgcag gcacagcggc cgctggtggt tgaggactcc aatccgggaa
    23461 tcaggagcgg acgcgccgcc ggcttcgatg tcgtccaggt tcgcgatgtt tccacgatgt
    23521 gccagcttgt ggccgaacgg ctgcaagacg gccgcattac gaaacagggt tgaccggttc
    23581 tccctcccgg agcggacgct cggtagccag agcgacgcgg tctccctcgg ctaacccggc
    23641 tgtaacctgg acttccgtga cgctcgatgc gccaatctcg accggccgcc actgtacacg
    23701 gccgtcgtgg agtacgaaca cgccgttttc ctgctcgcgg tggcgcagcg cttccttcgg
    23761 aatgacgagc gcgttttcaa ccacgtgcga ccgtatcgcg gcgtttacat tcgtgccggg
    23821 caacagctcg ccgttcgcat tgtccacgac agcgatcacg gtacccacct gccgggttcc
    23881 cagagtgatt acttccgtcg gaaccctttc cacttcgccc gtccacgtct gcccgggttt
    23941 ggcatcccag gtaatggaaa ccggcaggcc ttcgcgcact ctgccgagtt ccggttcatc
    24001 cacgtagatt tccacgcgca tcttgtccag tttgccgatg cgggccagct cgtcgccggg
    24061 attgacgaat gcccccacgc gaacgctcaa actgtatacc gttccgctaa tgggagagcg
    24121 gatcgtgccc tgttcgacgc ggcgccgtgc ggcgtcgagc gctatctgcg catcgcgtac
    24181 acgggcttcg gcaactgcga tgtcgctctc taccactagt gacgcccgct ttcgctcgag
    24241 cgcctcgata gcggcctccg cctgcgcgag gcgatcgcgc gccacggcca gttcctgggc
    24301 ggttgccgcc tttttggctt ggagcctttc gagagattcg tattcgcgcc gggcggcctg
    24361 cacctccagg cgcgcttcct tcatcgagct ctcgatggca gccaactccg gcgcgctccc
    24421 gcccttttga cggatttcga gttcagcgct tgcctgccgc gcgcgagcct cagccgcagc
    24481 gagatcggtc acggcatcga tggattccaa ttccaccagc gcggcccctt ttaccaccac
    24541 ctgtccctgt tgcaccagg
//
LOCUS       DASTMK010000298         7632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_16105, whole genome shotgun sequence.
ACCESSION   DASTMK010000298 DASTMK010000000
VERSION     DASTMK010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16105"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFQ79_12750"
     CDS             complement(<1..730)
                     /locus_tag="VFQ79_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0140583.1"
                     /translation="MRLARRSFLKSATAAALAPVLSAAPERPNIVLIYADDLGYGDLG
                     CYGSPIRTPNLDRMAHEGMRFTNAYSANPVCSPSRAALLTGRYPTRAGVPRVLFPRDT
                     EGLPDSETTIAQVLKPAGYKTMCIGKWHLGHHPAYLPTSRGFDEYYGIPYSNDMVPCP
                     LMHNTEVIEEPARQDTLTARYTEQAVRFIGKSKDSPFFLYMPHTFPHIPLHASKRFRG
                     KSKLGLYGDVIEELDWSVGEVLTAV"
     gene            845..2137
                     /locus_tag="VFQ79_12755"
     CDS             845..2137
                     /locus_tag="VFQ79_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140584.1"
                     /translation="MNRRYFLASSAAGALAMNSAASPNDTVRIAIVGLRGQGGSHVKV
                     YSAMRNVEIAALVDIDESILSKRVAELEGMGKKKPATYADIRKVLEDKNIDAVSIATP
                     NHSHTLQTIWSCQAGKHVYVEKPCSHNIFESRQIVAAAKKYNRLVQHGTNSRSGVALR
                     EAVQHLRDGLIGDVYMARGLCFKWRDTIGRTSPEPVPQGVNYDLWLGPAPKREFTRNR
                     FHYNWHWFWDYGNGDFGNQGIHELDILRWGLGVKYPVKVSAIGGHFMFDDDQETPNTL
                     TVTYEFNESGKKSMGVFEVRHWISNHEAGVGGEKPGNTVGNTFYGSKGYMVMDGYAKY
                     WTRLGREQQPGPERSEGGNNWANFIAALRSGKQEDLLAPIEEGAMSSTLMHLGNIAYR
                     LGRTLHFDAETMTVKGDAEANKMFTRAYRKPFVVPEKV"
     gene            complement(2141..2806)
                     /locus_tag="VFQ79_12760"
     CDS             complement(2141..2806)
                     /locus_tag="VFQ79_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEU0140585.1"
                     /translation="MPLPYEDRMEAGRVLAKELLQYANHRDVLVLGLPRGGVPVAYEV
                     AVRLNAPLDVFVVRKLGVPGHEELAMGAIASGGIQVLNTDVVARYAIPASAIAAAAAR
                     EIKELERRERAFRGGRSPLDVQGHTLILVDDGLATGSTMRAAVKALRERKPAKIVVAV
                     PVAAQRTCIELSAIVDELVCAETPESFQAVGQWYHDFSDPTEEQIRALLERAASRRNY
                     REP"
     gene            complement(2867..4174)
                     /gene="thrC"
                     /locus_tag="VFQ79_12765"
     CDS             complement(2867..4174)
                     /gene="thrC"
                     /locus_tag="VFQ79_12765"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU0140586.1"
                     /translation="MLSELVCFEPGCRARFPLDQVIYTCPQCGGLLEVAYSGDKPDPG
                     SLKKIWRERRMNNSPLDQSGVWRYRELLPFDGNAAGVVTLREGNTPLLNGPRAAAYAG
                     IDALVFKHQGFNPTGSFKDNGMTCGATQALRVGMRRVACVSTGNTSASMAAYASAAGL
                     QPVIFIPHGNISYGKLAQALEYGARTLQVEANFDQILALVRILAERLGIYLLNSINPF
                     RIEGQKSIIIEMLDQRDWTPPDWIVLPGGNLGNTSAFGKALRELVSLGFITRMPRLAV
                     VQAEGAAPFWDLMQSADRAALRPVEDPKTLATAIRIGDPVSWKKALQEVECSNGIVAR
                     ASEQEIADAKAQIGLCGIGCEPASAATLAGIKKLVSSGVIRPGDDVVAVLTGNVLKDP
                     DYIYRYHTGTLETPAGAPIKPSFGNSPIVVPNDAEKIAELLET"
     gene            4314..5777
                     /locus_tag="VFQ79_12770"
     CDS             4314..5777
                     /locus_tag="VFQ79_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140587.1"
                     /translation="MRSKASFAIPAAVLLAGGFFVLRETKAQQTRPSPETPAQRIANE
                     ARAAGIELRDDTSYLPVTDANFNEVMKKMRAAKPEIMKRQMSLLEQRYDLGNKPAPGV
                     TMSRGKPVQGGVRAKLPASVTWDQLAAMTPDEIRQKKAFPPGFLPLPHPNHAEGGMVF
                     PKFHIDEVKKQTDRDLTRFDLDFDLPDHLLPEFPPPIFLTTRPDLGDVSQGKLVTTEN
                     FFELFNGILNPKQLEGLRLLVTPFPQQQFNGTADRRSERPSLGVSCFECHANGHTNAS
                     THLVGDIRPQAFRHRIDTPTLRGVNIQRLFGSQRALRTVEDFTEFEQRAAYFDGDPVI
                     AAKKGVNVLDRGSQVHAMAEFQELLTFPPAPKLTVLGTLDPSKATPEELRGEKLFFEK
                     GKCGVCHPAPYFTDNSMHDLQTERFYSAQMINGRVAAADGPIKTFPLRGIKDSPPYLH
                     DGRLLTLEDTVEFFNLVLGLKLTTQEKSDIVAFMRTL"
     gene            6155..7465
                     /locus_tag="VFQ79_12775"
                     /pseudo
     CDS             6155..7465
                     /locus_tag="VFQ79_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1644 a         2149 c         2398 g         1441 t
ORIGIN      
        1 cgacggcggt cagaacctcg cctacgctcc agtcaagctc ctcgatgacg tcgccataga
       61 ggcccagttt cgattttcca cgaaagcgct tggatgcatg aagtggtatg tggggaaagg
      121 tgtgcggcat gtacaggaag aacgggctgt ctttggattt tccgatgaac cgcaccgcct
      181 gttccgtgta acgcgcggtg agcgtgtcct gccgggccgg ttcctcgatc acctcggtgt
      241 tgtgcatcag cgggcaggga accatatcgt tgctgtaggg gattccgtaa tactcgtcga
      301 aacccctgct ggtgggcaga tacgccggat gatgtccaag atgccatttg ccgatacaca
      361 tggtcttata gcccgccggc ttcaggacct gggcgatggt cgtttccgag tccggcaagc
      421 cctccgtatc gcgcgggaac agcactcgcg gcacgccggc acgagtcggg taacgtccgg
      481 tcagaagagc agccctggaa ggggagcaca ccggattggc ggagtaggca ttggtgaaac
      541 gcatgccctc atgcgccata cggtcgagat tcggcgtacg gatgggcgag ccgtagcagc
      601 cgaggtcgcc atagccgagg tcgtcggcat aaatcagaac aatgttgggc cgctccggcg
      661 cggcggatag cacaggagcg agggcagccg ctgtcgcgga cttcagaaaa ctgcggcggg
      721 cgaggcgcat aacaagagca tatactcaga agtaacggta ttaaacacgc ttgataaggg
      781 tggacgtttc gcgcgaagcg aacgggcgtt cgcacgggga aatttttgag atacgaggtg
      841 taacttgaat cgaaggtatt ttcttgcgag ctccgccgcc ggggcgcttg cgatgaacag
      901 cgccgcgagc ccgaacgaca ccgtgcggat tgcgattgtg gggctgcgtg ggcagggtgg
      961 ctctcacgtg aaggtgtatt ccgccatgcg gaatgtcgag attgccgcgc tggtggacat
     1021 tgacgaatcg atcctgtcga agcgggtagc cgagctcgaa ggcatgggca agaaaaaacc
     1081 cgccacctat gccgacattc gaaaggttct cgaagacaag aacatcgacg cggtctcgat
     1141 tgcgactccc aatcacagcc acacgctgca gaccatatgg agctgccagg cgggaaagca
     1201 tgtctacgtc gagaagccgt gttcgcataa catattcgag tcccggcaga ttgtggcagc
     1261 ggcgaagaaa tacaaccggc tggtgcagca tggcaccaat agccgctccg gtgtagcttt
     1321 gcgggaagcc gttcagcact tgcgcgacgg gctgatcggc gacgtttaca tggcgcgggg
     1381 gttgtgcttc aagtggcggg ataccattgg gcgcacgtcg cccgagccgg ttccgcaggg
     1441 agtcaactac gacctttggc tgggtcctgc tccgaaaaga gaattcacgc ggaaccgctt
     1501 ccactacaac tggcattggt tctgggatta cggcaacggc gatttcggca accagggcat
     1561 tcacgaactg gatattctgc gctggggcct gggagtgaag tatccggtaa aggtgagcgc
     1621 catcggcgga catttcatgt tcgacgacga ccaggaaacg cccaatacgt tgacggtcac
     1681 ctacgagttc aacgaatccg gaaagaaatc gatgggtgtt ttcgaagtac gccactggat
     1741 cagcaatcat gaagcgggag taggcggcga aaagccgggg aataccgtgg gcaacacgtt
     1801 ctacggctcc aaaggctaca tggtgatgga cggctacgcc aagtattgga cgcgtttagg
     1861 gagagagcag cagccgggtc cggaacgtag cgaaggcgga aacaactggg ccaatttcat
     1921 cgctgcgctg cgcagcggaa agcaggaaga tctgctggcg ccaatcgaag agggcgccat
     1981 gtccagtacg ctgatgcacc tgggcaacat cgcctacagg ttgggccgca cgctgcactt
     2041 cgatgcggaa acgatgacgg tgaaaggcga tgcggaagcg aacaaaatgt tcaccagggc
     2101 gtatcgtaag ccgttcgtcg ttcccgaaaa ggtctgatcc ttacggctcc cggtaatttc
     2161 ggcgggaggc ggcccgttcg agaagcgccc ggatctgctc ttcggtgggg tctgaaaaat
     2221 cgtggtacca ctggcccacc gcttgaaacg attcgggtgt ttcagcacaa accaattcat
     2281 ccacaatggc gctcagttcg atgcacgtcc gctgggctgc aaccggaacc gcgaccacga
     2341 tttttgcggg ctttcgctcc ctcagcgcct ttaccgccgc acgcatcgta gagcctgtcg
     2401 ccaggccgtc atccacaaga atcaatgtgt gcccctgcac atcgagagga gagcggccgc
     2461 cgcggaacgc cctttcgcgc cgctcaagtt ctttgatctc gcgagcggct gccgccgcaa
     2521 tggcggatgc aggtatcgcg tatcttgcaa ccacgtcggt attcagtacc tgaatgccgc
     2581 cggaggcgat cgcgcccatc gcgagttctt cgtgcccggg gacgccgagt ttgcgcacta
     2641 cgaagacatc cagcggtgca ttaagacgaa cggcaacttc ataggcgacg ggaacgcccc
     2701 cccggggaag cccgagaacc aaaacatccc tatgattcgc gtattgaaga agttctttcg
     2761 ccagaacgcg gccggcttcc atccgatctt cataggggag cggcatacgc ggacctaatg
     2821 accagacgcc cgcaagagtg gactgtttca gcgaaaaaca gcggcgctaa gtctcaagca
     2881 actcagcgat tttttccgcg tcgttgggta cgacgatcgg gctattgccg aacgatggct
     2941 tgataggggc tccggccggc gtctccagag tgccggtgtg atagcggtag atatagtcgg
     3001 ggtctttgag tacgttgccg gtcaataccg ctacgacatc gtcccctggg cgaatcacgc
     3061 ccgaggagac cagtttcttg attccagcga gtgttgcggc ggaagcgggc tcacaaccaa
     3121 tgccgcaaag gccgatctgc gccttggcat cggcaatctc ctgttcgctg gctcgtgcga
     3181 cgattccatt ggagcactcc acctcctgca gcgctttttt ccaggagacg ggatcgccga
     3241 ttcgaatcgc ggtggccagc gtcttggggt cttccaccgg acgaagcgcg gcccggtctg
     3301 ccgattgcat cagatcccag aagggcgcgg cgccctccgc ctgcaccacg gcgagccgcg
     3361 gcatacgggt aatgaacccg aggctaacca actcgcgcag cgcctttccg aacgccgacg
     3421 tattgccgag atttccgccg ggaagcacga tccaatcggg tggggtccaa tcccgctggt
     3481 ccagcatttc gatgatgata cttttttggc cttcgatccg gaacggattg atggagttca
     3541 gcaggtagat gcccaggcgt tcggcgagga tgcgtaccag cgcgaggatc tggtcgaaat
     3601 tcgcttctac ttgaagcgtg cgcgctccgt attcgagcgc ctgcgcgagt ttgccgtagg
     3661 agatgttgcc gtgaggaatg aagataaccg gctgaagtcc ggccgcgctg gcgtaagccg
     3721 ccatggatgc ggacgtattg ccggtagaga cgcaggcaac gcggcgcatg ccaacccgca
     3781 aagcctgcgt ggcgccacat gtcatcccgt tgtctttgaa cgagccggtg ggattgaagc
     3841 cctggtgctt aaaaacgagc gcatcgattc cggcataggc ggcggcccgc gggccgttta
     3901 gcagcggtgt gtttccctcg cgaagcgtga ctaccccggc ggcattgccg tcgaacggaa
     3961 gcagttcccg gtaccgccac acgccgcttt gatcgagcgg cgagttgttc atgcgccgtt
     4021 cgcgccagat ctttttcaat gatcccggat ccggcttgtc gccagagtaa gcgacttcca
     4081 acaggccgcc gcattgcggg cacgtataga tcacctggtc aaggggaaag cgggcgcggc
     4141 acccgggctc aaaacagact aattcggata acatgaaggt ccattttcgc acgcgatata
     4201 actattgacc tcagcttgcg tttgccataa tgttgcatca gcggcggtga accgccacgt
     4261 cgcgagggcg cacccatgag cccttttcca aaatccagtg aaggaggtac gtcttgagga
     4321 gcaaagcatc attcgccata ccggccgccg tgctgttggc aggcggcttc ttcgtgctga
     4381 gagaaactaa agctcagcaa acacgaccct cgccggaaac tcctgcgcag aggatagcga
     4441 acgaagcccg ggccgccggg atcgaattgc gggacgacac aagctatttg cccgtcaccg
     4501 acgccaactt caatgaagtg atgaaaaaaa tgagggcggc aaaaccggaa ataatgaagc
     4561 ggcagatgtc gctgctcgag cagcggtacg acctgggcaa caagccggcc ccgggagtga
     4621 cgatgtcgcg ggggaaaccc gtgcagggtg gcgtgcgcgc caagctgccg gccagcgtca
     4681 cctgggacca gctcgcggcg atgacaccgg acgagatccg gcagaaaaag gcgtttccgc
     4741 cgggatttct gccgctgccg caccccaacc atgccgaagg cggcatggtg tttcccaagt
     4801 tccacattga tgaggtgaag aagcaaacag accgcgacct cacgcgcttc gatctggatt
     4861 tcgatctgcc ggatcatctg ctgccggagt ttccgccacc gatcttcctc acaacccggc
     4921 cggaccttgg cgacgtgtcg caaggcaagc tggtaacgac cgagaacttc ttcgagctgt
     4981 tcaacggtat tctgaacccg aagcagttgg aggggctgcg ccttctggtg acgccgtttc
     5041 cgcagcagca gtttaacggc acggccgacc ggcgctcgga aaggccgagc ctgggcgtca
     5101 gttgcttcga gtgccatgcc aacggacata ccaacgcgtc gacgcacctg gtgggagaca
     5161 ttcgcccgca agcgttccgc catcgcatcg acacgcccac gctgcgtgga gtgaatatac
     5221 agcggctgtt cgggtcccag cgcgcgctcc gcaccgtaga ggattttacg gagtttgagc
     5281 aacgtgccgc atacttcgat ggcgaccctg tcatcgcggc gaagaagggt gtgaacgtcc
     5341 tcgatcgcgg aagccaggtc cacgccatgg cggagttcca ggagctgttg acttttccgc
     5401 cagcgccgaa gttgacggtg ttggggacgc tcgaccccag caaggccaca cccgaggaac
     5461 tgcgcggaga gaagctcttc tttgagaaag ggaagtgtgg cgtgtgccac ccggctccct
     5521 acttcaccga caacagcatg cacgatcttc agacggaacg gttctattcc gcgcagatga
     5581 tcaacggcag agtggcggcc gccgatgggc ccatcaaaac tttcccgctg cgcggcatca
     5641 aggactcgcc gccttacctg cacgatggca ggctgctgac gctggaagat acggtggagt
     5701 ttttcaacct ggtgctcgga ctcaagctga ctacgcagga aaagagcgat atcgtcgcct
     5761 ttatgcgcac gctgtagcaa catgctgaga aagggagttc ggggtccgct ccgtcgaaga
     5821 cgtgctgtaa tttgttgatt ctgcgtgagg ccgctcgtgc gtcttcatgg ctattggcgg
     5881 cccgcagggc catggggact cccctggcgg tcgcggctca gcggggcccc gggcattgcg
     5941 ccggatggaa cggagagaga ccgttgtcgg acgggcgagg cagtgcagtt aaactgcgag
     6001 agaacgcgat tgcagtcacg gcccttttgt cattggagcg gtttgcccct tgctcagcct
     6061 ggctccagga aggcatggaa cattggaggc atcccgttgc actccccgag agtgcgtgct
     6121 ggagccggac ggtctacccg tgctgcgtgc aaacgtggca ggcgtcgacg tcgggagcca
     6181 aagtcattgg gtatgcgcgc cgaccctgga tggcggcggg cgggaagtaa aagagttcgg
     6241 ggcgacgacg ccagagttgg agaaacttgc cccgtggtta caggagcgca aggtcgaatc
     6301 tgtagcgatg gaaagcaccg gagtgtattg gattccgctg cacgagattt tggaatagca
     6361 gggattggaa atactgctgg tatgcacccg cgacttggcg caggttcccg gccggaagaa
     6421 gacagaccgg atagactgca agtggattca acggctgcat agttgcgggt tgctaaaagg
     6481 atcgttccgt cccgccgaac agatctgcat gttgcggact ctggtgcgcg ataagaacaa
     6541 cctgatgagg gaacgagccg actggattcg acgcctgcag aaaagcctgg accagatgaa
     6601 tgtccgcgtg caccgagcgg tctccgatat tcagggaacc accgggatgg ctatcatccg
     6661 agccatcgtg agcggccaac gggacccagc ccagctagca cagttacggg atccggggtg
     6721 ccgtcgcagc gccgaggaga tggccgagca actgagcgga aactggcggg aagattatct
     6781 gttcagtctg gagcagagcc tgcagatgca cgatgccatt gtggaaagaa ttcaggcata
     6841 tgatcatgag attctgcgcc gcctggccca gttggagcca ccagacaatc aaggcaaaac
     6901 tgctcccacc ttgcccaacg ccaacaaagc gaaaatgatt cggcaacgcg gggaagaacc
     6961 ttttcgacag gctttttatc gcctgagcgg gacggatttg accgcgatcg acgccgtcgg
     7021 cgtcggagtg gcccagttgg tgctcgccga atatgggccc gatctgagcc gttttcccac
     7081 cgagaaacat ttcatctcgc ctgtcacgct ggcacccaaa cagcgggtga gtgggggaaa
     7141 gccactgaag aagaaaaagt ctggcagcgc gagcagtcgt gtgtcggcag cgctccgcag
     7201 tgcggctctt tcgttgcgac acagccgcac cgcgctggga gcttactttc gccacaccgc
     7261 caatcgcctg ggagcagatg tggcggcctt cgcgactgcc aggaaattgg ctgcctggat
     7321 ttaccgctcc ctgcgctggg gacaggccta cgttgacgaa ggagccgaag cttatgagca
     7381 gcgatgtcag gccgcccgta tccaccgtct acgtactcat gctgcccagc tcggatatgc
     7441 cctcgtggag acggtctcag agtagaaacg gagccgcgcg cgagcaagtt tcgcatgatc
     7501 cttcggctca caacctgaga tgaaagtggg tgtaggttgt gatgagacgc ggctgcggat
     7561 cgaggccgta aaccgcaaca gggcagtgaa gcctgccagc aagtttggtc tgggcaccgc
     7621 ttggaacaac gg
//
LOCUS       DASTMK010000299        54656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_46674, whole genome shotgun sequence.
ACCESSION   DASTMK010000299 DASTMK010000000
VERSION     DASTMK010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 54656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54656
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46674"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..573
                     /locus_tag="VFQ79_12780"
     CDS             220..573
                     /locus_tag="VFQ79_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140588.1"
                     /translation="MRACRSAIADLEQILILRADWSEPYALGASILAGCPDSNLRDPK
                     KAIAWAERAISLDKNRPTLLTILALAYASDGQLDKAVAAQRRALNSPNFPMPYRENAE
                     HQLHKYEADLSRQGK"
     gene            complement(738..1202)
                     /gene="arsN2"
                     /locus_tag="VFQ79_12785"
     CDS             complement(738..1202)
                     /gene="arsN2"
                     /locus_tag="VFQ79_12785"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HEU0140589.1"
                     /translation="MPSEILIRPATAADLEAVSELLRSARLVPIDDTAQFGDQYAVAA
                     AVDGTIVGMAGYERYGTDILLRSVTVDEKWRSVGIGHRLSLDRLEHASVRGCTTAYLL
                     TDTAAEYWKRHGFVQIDRSAAPVAISHSREWSAICPASAIAMRRHLPSCTAC"
     gene            complement(1202..2059)
                     /locus_tag="VFQ79_12790"
     CDS             complement(1202..2059)
                     /locus_tag="VFQ79_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEU0140590.1"
                     /translation="MNTSTDIKEVVKEKYGQAALRVQSGKTSCCGGGSALGASCDPIT
                     SQIYSAEEGANVPQEALLASLGCGNPTALAKLNPGETVLDLGSGGGIDVLLSARRVGL
                     AGKAYGLDMTDEMLALARENQKKAGIENVEFLKGEIENIPLPDNSVDVIISNCVINLS
                     ADKDKVFHEAFRVLKPGGRLAVSDIIVRGDMPAEVQRNVELWVGCIAGALRDTDYISK
                     VKAAGFQSVEIEPTRVYTADDARQLFSGRELDVEAIAPQIDGKFASAFVRATKPAACC
                     GPSCCSGTK"
     gene            complement(2145..2465)
                     /locus_tag="VFQ79_12795"
     CDS             complement(2145..2465)
                     /locus_tag="VFQ79_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0140591.1"
                     /translation="MSRYADMMAAMGAEPRLRIMRLLLSAHPRGMVVGDISDGLEIPG
                     STLSHHLDKLKNEGLVTVKRESTFLRYAANAEALQDLLGFLYAECCTRNQAVEPEAIV
                     QISR"
     gene            2723..3070
                     /locus_tag="VFQ79_12800"
     CDS             2723..3070
                     /locus_tag="VFQ79_12800"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0140592.1"
                     /translation="MKIKDDPICCPPVEPQVYGTLPGLEGPDADEELAKLAKAIGHPA
                     RVRILRMLSQKEARVCSQIVDELPLAQSTVSEHLRILKDAGLVRSSQDGPRIGYCINY
                     GTLRKLKALVAII"
     gene            3121..3315
                     /locus_tag="VFQ79_12805"
     CDS             3121..3315
                     /locus_tag="VFQ79_12805"
                     /inference="COORDINATES: protein motif:HMM:NF018637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HEU0140593.1"
                     /translation="MKKVEVFDPPMCCSTGVCGPNVDPTLVRFTADLHWLANQRVAVE
                     RSQEKAVSARHHSPGRQQSR"
     gene            3222..3602
                     /locus_tag="VFQ79_12810"
     CDS             3222..3602
                     /locus_tag="VFQ79_12810"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEU0140594.1"
                     /translation="MVGKPAGGGRTITGKGGVGKTSLAWAAAVSLAQRGKKVLLVSTA
                     AHVATTIGGAAPNLSVSGINPARETRQYTEAVMAKSAPHLDAQGMALLEEDLRSPCTE
                     EIAVFSAFARTVAEGSGPTGSENW"
     gene            3646..4731
                     /gene="arsB"
                     /locus_tag="VFQ79_12815"
     CDS             3646..4731
                     /gene="arsB"
                     /locus_tag="VFQ79_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEU0140595.1"
                     /translation="MENRSTPRLSFLDRYLTLWIFAAMALGVGLGYLVPGVVPFLNRF
                     SVGTTSIPIAIGLILMMYPPLAKVRYEEMGRVFQHRRVLLLSLIQNWVIGPILMFALA
                     VLLLRDKPEYMTGLILIGLARCIAMVIVWNDLARGDREYAAGLVAFNSVFQVLFFSVY
                     SYFFLATVPRWLGLTAVEVDISMGEIAKSVFIYLGIPFIAGMLTRFVMLKAKGREWYE
                     KSFIPKISPITLISLLFTIVVMFSLKGEMIVQLPFDVVRVAIPLTIYFVLMFFASFWM
                     SKKVGANYPEATTLSFTASSNNFELAIAVTIATFGIHHGAAFAAVIGPLVEVPVLISL
                     VNVALWINRKWFGGVFASVDTCQTQRA"
     gene            4758..5183
                     /locus_tag="VFQ79_12820"
     CDS             4758..5183
                     /locus_tag="VFQ79_12820"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEU0140596.1"
                     /translation="MKKVLVLCTGNSARSQMGEGLFRHEGAGRYEVESAGTKPGDVRP
                     EAIAAMKEIGIDISDHRSKSVNEFSGQSFDYVVTVCDNARDNCPVFPGKAERIHWSFE
                     DPTAVEGDEEVRLAAFRRIRNQIHERVKAFVRKRAALDR"
     gene            complement(5383..5838)
                     /locus_tag="VFQ79_12825"
     CDS             complement(5383..5838)
                     /locus_tag="VFQ79_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEU0140597.1"
                     /translation="MRPRGKQPRGVVDTSVLIAGIAGFKSQRTVENPSAALIRDWVED
                     GTFIWLLTEEIVSEYKGVLTRLVVRRSLVGEIINLLREEAEFVEVRSGSDVSPDPADN
                     AFCACAEEGRAAFIVTLNRKDFPQKKLVAKVISPGDPIPTTRRRRSSRV"
     gene            complement(5813..6127)
                     /locus_tag="VFQ79_12830"
     CDS             complement(5813..6127)
                     /locus_tag="VFQ79_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140598.1"
                     /translation="MTSVRYLSLRSRPSRGREPIEVRIPVRDLAKELEEQLPLAAVYK
                     DLPRGRKRPGWKREFTRSLAAAADELSGVTETQIAEAIRGFRAEKKRLPEITVAPSRK
                     TA"
     gene            6456..6626
                     /locus_tag="VFQ79_12835"
     CDS             6456..6626
                     /locus_tag="VFQ79_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140599.1"
                     /translation="MLFEIRLPADKLNGDIQITNPFRFADVAPEYYRFLNSRQEVRIT
                     IQGCDALEQFRQ"
     gene            complement(6860..9823)
                     /locus_tag="VFQ79_12840"
     CDS             complement(6860..9823)
                     /locus_tag="VFQ79_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0140600.1"
                     /translation="MIRFDQYRLDPVQGLWRGSIDVRITPKSLALLCVLAERTGEVVT
                     KEELFRAVWPGTAVTDSALTSCIRELRSVLGDTARRPRFIETLHRRGYRFRAPTLTGS
                     PDGGPNVGPPAPIHTDAPLIGRELVFAGMLDAFTRAEHGSRQVLFVTGEPGIGKTTVV
                     RAFLARARSRDNVRATWGQSIEHYGVGEPYQPLLEALTRLCQQPGGDRIVHLLERYSP
                     TWLAQIPALLAPEHLVSLQRTAAGTTRERMLRELNDAVEAITAEAPLILWLEDLHWSD
                     MSTLDWVVAFAQRPEPARLLLIATFWPPAVSGTEHPLAEVVERLREKGLSREIALGGL
                     DEGGVADYVALRFPPATGHNERMRQLARVVQQHTGGNPLFVVNILGDLVERGLLLEQD
                     GRWTVSGDAGEPALGIPDGLRRAIERQVNRLLPDERFILEIASGAGATFSAAAVAVAA
                     GRELDEVEITLTALARQRRFVQEGVAVEWPDGTISAGFDFLHVLYRDVLYQRIPASRR
                     ADLHWKIGERQETAYGQRSPEIAAELAMHFERSRDLHRAGIYRQHAAENARRRNAYKE
                     AQLHFKKALMLLANEPPGQERTEHEVALQIGLGAVLMATHGWGAPEVEHPYSRAYELC
                     HGLGDTPRIFPALWGLWLYYLGQGPLSIAHNLAQDLMTLARRHDDRQLLLQAHHSGWA
                     TAFLRGDLDAARIHSTSAIGIYQVERHAAMAATYGSHDAAVCGQYFLGLTLALQARMD
                     EAARACNQCIALAQELSHPMSLALAQVFTAAVGQMQRDSDAVRIHAEAAVATAREQDF
                     RLILGWAWPLEGWAAIEHQQYREGLDLISRGLAEVRATGSNSWMTYLLGLHADALLKG
                     GQAVAGLQALDEAFAVSGRTGERFWEAELHRLKGELLLAASASSAKHEAEQVFLRAIE
                     IARSQGAKLLVLRAAVSLGRLWCRRGRRAEAGLIVAEARRAIDGPAFPDMVEADSLLA
                     ESGGRAAPKSPPMDL"
     gene            9985..11217
                     /locus_tag="VFQ79_12845"
     CDS             9985..11217
                     /locus_tag="VFQ79_12845"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEU0140601.1"
                     /translation="MAAMLFTSALTRADVVSDWNRVMLTTLSAQNPYIQARVAAITHL
                     AVFEAVNAVTGEVKPYLGTVTASPGASAEAAAVSAAYTVLIKYLPDWADALNDNRADS
                     LARIPEGPAKSAGIAAGEAAANAMIAARASDGSEPPEVYTPPPSPKPGEWQLTGACNG
                     GVFLHVAKVTLFALRSSDQFRSDPPPALASGKYARAYNELKEFGGVNSPGRPQDRADV
                     ALFYSKVLSTFIWNPVATQIAARQNKSLSENARAFALLNMAIFDAFVANFDTKYRYPF
                     WRPETAIHAGDQGGNRNTEPDPGFAPFVPTPCHPSYPAALPALGSAARVVLERIYGRR
                     GHSITLSTPAVPNVTLHYENLRDISDDINDARLFGGIHFRFDDAAGNEQGRQVGDYVF
                     RHKLRRAQSCASGEEEDQ"
     gene            11214..12857
                     /locus_tag="VFQ79_12850"
     CDS             11214..12857
                     /locus_tag="VFQ79_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEU0140602.1"
                     /translation="MMLHCTSALTMGNRRRPLPAFAATWSQALLAAGTITIFATSGLA
                     QRADFNGDGFADLAIGAPSIGREGRVRVIYGSFTGLTGTAGPGDQSWRQGHSGLGGIP
                     EPGDNFGAALATGDFNNDGYGDLVVGTPGEDDNSGELRVLYGSARGLQVVGVGAPPEK
                     WIRRGLPGVEGTRRLDDRFGDVLAAGDFNNDGYDDIAVGAPGFFSANPDRYAEGSVSV
                     FYGSVTGIQFNGQGGPDDQFWKQGLNGLQGVSQRHDHFGNALTVADFNRDGFADLAIG
                     IVSNPGGGALSVLYGSAAGLQATGSGAPDDQFIGRDSPGVKGHGGEGGFGAALTAGDF
                     NGDGFADLAVGAPFPFERTGSLNVLYGSPSGLQATGSVGPDDQLWSPDSADVAGTGTP
                     GEKFGASLTAGDFNRDGFADLAIGAPYKSRSGLLWYGQVYVLYGSGAGIQAGTNGPED
                     QLWDRGSIDLQGPAAASQDFFGFALAAGDFNNDGCADLVIGNGAIGVQANGVHVLYGS
                     PGNGRQNGVQATGNGAPDDQFFTPINAGEGFGRALAASN"
     gene            12906..13127
                     /locus_tag="VFQ79_12855"
     CDS             12906..13127
                     /locus_tag="VFQ79_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140603.1"
                     /translation="MEIATSHGNPVTIAEADLDDLRIAMRGSLLRPGSLGYDEARVVF
                     NGMFDRHPALIARCRGVTDVVDAVRFARE"
     gene            13152..14090
                     /locus_tag="VFQ79_12860"
                     /pseudo
     CDS             13152..14090
                     /locus_tag="VFQ79_12860"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            14096..14353
                     /locus_tag="VFQ79_12865"
     CDS             14096..14353
                     /locus_tag="VFQ79_12865"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEU0140604.1"
                     /translation="MGDWRDARETPRHVAWVRQAWNRLGPHSTGTVYLNYLGSEDGEV
                     DTLVRSAFGSNYDRLVEIKAKYDPTNFFQLNPNIKLVSRMT"
     gene            14469..15260
                     /locus_tag="VFQ79_12870"
     CDS             14469..15260
                     /locus_tag="VFQ79_12870"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU0140605.1"
                     /translation="MRTVGTIESLWRYPVKSMAGEALTEAFMGFSGVYGDRCYAFKSS
                     AARKGFPYLNAAAQQRMLLHHPKFRYPERAAKPPNLIEAMSIKPGVTPANAEPDDLML
                     DVVTPFGAVVGIDDASLTKSLREGLRGEHHLTLVRSDRALTDCRPISVISLQTIRQIE
                     EESRIAIDKRRFRANVYFNLTSNESFAEDELVGRNLRLGSKAVIAVLERDPRCKMISL
                     DPDTGEHNPAVLRKVAQAHEAFAGVYCAVLVEGIVAVGDSIEAVG"
     gene            15397..16824
                     /locus_tag="VFQ79_12875"
     CDS             15397..16824
                     /locus_tag="VFQ79_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0140606.1"
                     /translation="MKISPASRTPGNSLSNLPGPRGLPLLGNLLQLDVTQLHVILEEW
                     ANVYGPLYTFKIGSRPVVAIAEAALIHEVLRGRPEMYRRLGSIARVLTEAGVNGVFAA
                     EGASWRRQRRMVTQALDNEHLRRFFPTLARITGRLKSRWDRVACKACATDVQQDLMRY
                     TVDVTTNLVLGYDGNTLENEDDVLQRHLEHLLPALNRRINAPVPYWQFVRLPADRAFD
                     DALRVVRLLIADLVAQCEARLKHAQGEGYHPTNLLEAMLAAREQEGAFTREEIVGNAL
                     TMLVAGEDTTAHTMAWMIHLITGHQQVQKRLQQEVDEVLGQASMLEDFEVQERLTFLE
                     AVAHETMRLKSVAPVLFLETNHAVELGGVHMPEGTALFLLTRQCGLQECAFAMPTEFQ
                     PQRWLAGAELSPGQRTSNAFMPFGDGPRFCPGRSLALLEIKAVVAMLCRNFCISKAEA
                     AKEVGEHFAFTMMPTNLVVTLRTRE"
     gene            complement(17289..17915)
                     /locus_tag="VFQ79_12880"
     CDS             complement(17289..17915)
                     /locus_tag="VFQ79_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140607.1"
                     /translation="MKISPAARTILDHSSHSQPSPATAAVAGVADGFRDAFIGNSARE
                     VISMQLTFSRVLTLMVVSIMTSSLNVQQFTQSEGNDMTGSWLISLNRVLPSPVQALAL
                     GTFNKEGTFIATAQGDGTFLQGLRTEGPAHGAWKRTGFNIFALTFHTLWFMPDASLTG
                     IMTVNMHLTLHSMTDRISGQFSGQLASPGGNLIFSLKGTFTGQRIRVQ"
     gene            17887..19995
                     /locus_tag="VFQ79_12885"
     CDS             17887..19995
                     /locus_tag="VFQ79_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140608.1"
                     /translation="MVRAAGEIFMPNGGTNNARDPKQPAPGDRLDSWKEIGAYLKRSV
                     RTVTRWEQEQGLPVYRHKTGAVYAYKPEIDAWWAAHRKQIQVEIDNESAVALSPRKAR
                     SPWIVVSAGVAALSMAVALGWFAFRSPPRRTAKSVPLTAYPEIEGPPSLSPDGNQVAF
                     HWKGDIWVKAVDGDGVRNITDTPGAEEASPAWSPDGRWISYVKNRAEVMVSTPLGGTT
                     TRIASIKASPWGGTLAWVPDSKSLVIAEVPSEGNKIQAHLAMVSIATGEKKQITFPPG
                     PGIGHFWPTVSPDGRSVAFAEFPQDSSANIYIQPLDGGVPRRITDDKANIRGLSWTPD
                     GRELVFASDRNGVFRLWRMTAQTAAQGTPAGLVRLLSVFLNMRSRPASAQIVEGAGED
                     ARFPSFSRPGPGRPVRLAYQQYRENQYIRRTALAGDSSSRMSSTSERLLYSSRSESEP
                     QFSPDGHKIAFTSDRSGAWDLWVSDGQHEPVRRASMEGPPVLGPRWSPNSRQLAFFAP
                     TGAAGNYQIYVVDESDGKPQLLSRDERYADFLPSWSHDGQTIYFGSGRGGKTQIWKMP
                     ARGGNPVQVTKGGGGDAWESLDGRLLYYTKVPEEGPGLWSVPVDGGEEHKVLDGPWFK
                     GWALTQKGIFFGDWAAEGDGSKPVKFFTFKTLPITIGSVEKTDTGYLPSLTVSPDGRW
                     LLYTSRERVDADLMLVDSFQ"
     gene            complement(20268..21260)
                     /locus_tag="VFQ79_12890"
     CDS             complement(20268..21260)
                     /locus_tag="VFQ79_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140609.1"
                     /translation="MKRLAVPGRVLVGLFAAVLTYGQAPSRFELLAKFRGLGGMYAST
                     VGPGPAAGGERLYLSYLYLANTVDVVAVDPATGDAQVFRNPAEGEHGARCMTVGPDGN
                     IYLGTLPRAHFLKLDVKAGKLIDLGRPSKTEKFIWDVAFGADGKLYGGTYPGAKLLRY
                     DPATGALEDLGRMDPTEHYAHYVAGSDDGFMYAGIGYSRANITAYEIATGKHRGILPA
                     GAQVTGQAVVYRGTDGKVYGSIAGRYFRLSGWIATPVSEAEAQRRAANRLRDGRTVEV
                     RGNTLRVHNPKTGASETRTFSYEGNSLPLLKSPVGMHGCSAPTRPQRPATVSMS"
     gene            complement(21257..22543)
                     /locus_tag="VFQ79_12895"
     CDS             complement(21257..22543)
                     /locus_tag="VFQ79_12895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0140610.1"
                     /translation="MKIAIEGRTPVRSQPLPLEYPGVHYMDEEEVEAAVRVLRSRSLF
                     RYYGPEPQNETGLFEEEFARFLDVPYALAVSSGTGALHTALAALGVGPGQEVIVPAYL
                     WVSIVGAVVSRGAIPVLADIDDTFCLDPADVERRITPRTAGIVLAHMSGAPGDAPALA
                     ALARKHGLFLVEDCAQCAGGALGGRKVGTFGDIGVFSFQLNKNMTCGEGGCVVTGDAG
                     LYRRAVACHDVGYARDVNGRLILNPSDLSLWGMGYRMDELRAAVLRVQLRKLPRIVCA
                     MRRSKYRIRRELERLPGVKLRRVLDSAGDTGCFLISSFAGAEEAREVNAALRAEGIVT
                     SPGGISNILMTNWGLHLYSNNASLVDLASTDGRGFPWTLADNSGSALRYAKGTCPVAD
                     SLFERSILLAIPSCLTAADEDDIVSAFQKVVPAGVR"
     gene            complement(22603..23586)
                     /locus_tag="VFQ79_12900"
     CDS             complement(22603..23586)
                     /locus_tag="VFQ79_12900"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0140611.1"
                     /translation="MAREPRNGATLRNLDFVAPPAHAHPARRQSLERISVAPLRIWAA
                     SAGKWRLYWRPMRIAVPLIALLMAGAAPGQQTGFRWPDGAKAAVALTYDDGVDVQLDH
                     AVPDLEAARLRGTFYVTGYSESFRKRMAEWRAIAARGHELGNHTLFHPCLKQADGRER
                     TWVDAERDLNTYSIARIAAEFRVMDAFLHAVDGEKAHTLAYTCGDETIRGVSFVDSIR
                     PLAPAARAYKHAFRALADPLELDPYRVPSWALANDKGAEMIAWVEQAVQSGALAVFTF
                     HGVGGGHSINVDREDHQALLRWLDANRARVWTAPFVKIMAHVTAERRRLAR"
     gene            23604..25649
                     /locus_tag="VFQ79_12905"
     CDS             23604..25649
                     /locus_tag="VFQ79_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU0140612.1"
                     /translation="MPRFLLALAVFILAWAPLLDGAVRIRRDISDNWQFLPVDEAGME
                     NTDYLSRVGWLTVGLPHTWNTKDIFDNVPGYRRGPAWYRKSFDIPAEWRGKRIVLRFE
                     AAAQVATVWVNNTLVGEHKGAFTPFEFDITRLARVGGDNLVAVRVDNRWRRDVPPYDM
                     DFNMMGGLHREVYLIATASAYIASTRVTTPRVSEGEAVAAFEAEVRNEASTQREVEIV
                     TQVLDSSGAPVETLTSKAAILAGAAHTFRQETRPILKPRLWSPDTPNLYRVRFEIREQ
                     GAAIDDDESPLGFRWFRFDPDKGFFLNGAPLKLRGANRHDDYPGLGWALPPSRHVADL
                     KLLKEIGANFLRPAHYTQHPVVLETCDRLGLLVWEEVPFDGEGEQLAPYAGASGFAET
                     VKQILRETIRRDRNHPSVIVWSVGNENLNGATENEWRAVAGLTKELVALAKEEDPTRP
                     TGVAINRFDRAEHVGLMELVDVVGLNVYYGWYGQTFEDFPPLLDALHRKHPTKPLIVT
                     EYGADMGRGLHSDTPRRYDFSEEWGCLYHEAYLREIDARPFVAGSLIWNIFDFAVEKR
                     MAQTIPHMNQKGIYDYYRRPKDVYYFYRSRWTEEPMIYVVSHTWTERQGAPGEPKQFK
                     VYSNCDRVELFVNGKSAGVQPGGAPVWKVLLASGDNEIRAVGRKGEREVTDRLRVRY"
     gene            25832..27130
                     /locus_tag="VFQ79_12910"
     CDS             25832..27130
                     /locus_tag="VFQ79_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0140613.1"
                     /translation="MLGRIRGLRWWVIFTCFLATTINYLDRQCLSVTAAVLSKEFGLS
                     NADYSSIITFFLFAYTLMQAVSGRIIDRIGVRTGMALSILIWSFAGILHAFAQGLWSL
                     RIFRFLLGFGEAGNWPAATKVVSEWFPGRERALAVAIFDSGSAVGGIVAAPLIAALTL
                     HYGWRPAFLITGSLGLVWLWLWLWIYRKPEEHPRITKEELQWIRQGNANQLEPQGAVV
                     TWTGLLRVRAVWGVILGRFLTDCVWWFYVYWLPKYLADVRGFTLSQIGAMAWIPFVAV
                     DLGNVGSGWISGRLMRRGFSLNAARKAVLLLAAAGMVAALPAGTTASAALCLGLIAVA
                     CFSYAAWGTMMLTLPCDLFDARMVASVSGLSGMGAGLGGILFTWAIGVVVDRFSYQPI
                     FVAAGLLPLLAMLTVQCLIPEVRRMAGAGAAAHRSGEGVS"
     gene            27161..27487
                     /locus_tag="VFQ79_12915"
     CDS             27161..27487
                     /locus_tag="VFQ79_12915"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU0140614.1"
                     /translation="MTRCFQIHPADNVATLLADIVEPGVAGILGGSIGSLEVRENVPF
                     GHKVAVSRIAAGDAIVKYGTTIGVATREIEPGEWVHLHNCRSRVDEKSSQLDVQTGAR
                     TETVYE"
     gene            27480..28685
                     /locus_tag="VFQ79_12920"
     CDS             27480..28685
                     /locus_tag="VFQ79_12920"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU0140615.1"
                     /translation="MSEPVWQGHLRPDGRKGIRNLVLVVYTVECASHVAHAIASGEEA
                     THVIGFPGCYDNDYAIRLILALARHPNVGGVLAVGLGCEYTRPERIAGVARASGRPAE
                     WFTIQEAGGTRASIAKGKALVAGLRDQMSRSDVPVPMTFADLTVGAECGGSDATSGLA
                     ANPVVGAFFDLLVDRGGRAIFEETVEMIGLRDTLRSRAASPEAERQLVASYDKAVNYC
                     RSVRQYSISPGNFAGGLTTIEEKSMGAFSKSGSRPIQGAIRVAEAPPGPGLWLLDSVP
                     DEYFMGFGYTNPNDTEGVMDLLSAGAQMVLFITGRGSVIGSPIAPLVKITGNSQTFRR
                     MRDDMDFNAGRILEGELSMEAAARELGGLVARVAAGEATCSEALNHREYFIMYKHQDT
                     PALAEGCRA"
     gene            28678..29457
                     /locus_tag="VFQ79_12925"
     CDS             28678..29457
                     /locus_tag="VFQ79_12925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0140616.1"
                     /translation="MRKPFRNTQEAFGLDGETALITGGGTGLGFAMAQAFVASGCHVV
                     LAGRRETLLAEAVARLGPQASHRVHDVSRHDQAGPFADSLEKQFGFISIVVNNAGIHL
                     KKAAAETTEAEFSAVLDTHLTGAFAITRRLIPAMAERRHGSILFIASMTALFGMPHVA
                     AYSAAKSACVGLTRSLACELAPRGIRVNAIAPGWIDTPMLREALARDPERERRILSRT
                     PQQTFGSPEDVAWAAVYLCSPAAGFLDGVVLPVDGGASIGF"
     gene            complement(29466..30527)
                     /locus_tag="VFQ79_12930"
     CDS             complement(29466..30527)
                     /locus_tag="VFQ79_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0140617.1"
                     /translation="MKVTKPTLNAVARRANVSASTVSRLLRGTMRVTPEIEERIRRAA
                     EELGFSLNRENGTRIITFLFSNRDVLHYFHSLVLASAEVYLARRDYNLLYLSLRYSQT
                     TPWREVHLPKLLQRRDLVSGFIVAGTNTQNLFDLLAHRKVPFVVFGNNVAGPWKQDKI
                     DSVWSDDVQGGMDVTRCLLTLGHRDIWYVGNTRLTWHAHIYEGYCRAMTDAGCEPRLS
                     EFDSEDEHELGYLTTKSILNRGLPVTALFAGGDVVARGVYQALAEAGLRVPADVSVAG
                     YNDIEAENMRPPLTSVRVFSEQIGKALAELLLNRVSQPDLPPQRRTIPTQVILRESCA
                     PPLAGTAGPPARQVLASQP"
     gene            30785..34222
                     /locus_tag="VFQ79_12935"
     CDS             30785..34222
                     /locus_tag="VFQ79_12935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0140618.1"
                     /translation="MRRSVRSLLGSLCLFAAVFTSPQSAIAQDARGMITGVVTDASGA
                     LVPEAVLRAVNTASNVAVTAATNAQGNYTLNHLLPGEYRIYVSKEGFKTFERGVEVRA
                     ADRMTLDVQLEIGAASERIVVEAGTPLLEVASSSLGTVIDHRRIQDLPLVHGNPFMLE
                     FLSPGITFNGNIAWTRPFDGPASVASVNGSQTARIEFQLDGVANNWRRSPAYQPSVEF
                     IQEYKVQTTSYDAAQGHSAGGWVDVALKSGANDLHGSAYYYRQDPALNTNLFFNNKAG
                     QPKPDYMFYRTGATAGGPIRRDRTFWFVGYERIRHNLPYPQVYTVPTEAQRNGDFSAL
                     LALGGQYQLYDPATTRPAEAGRYSRQPFPGNIIPASRQSGIGKNIVNYYPLPNQPGSR
                     DGGSNFNFGSGIEPDHYYSFTTRIDHTFSDRQRIFGRAVVSKRVDGPYRDWAPDASGN
                     NLYYKNRGAALDYINNLTPQTVLNLRYGYTRFVSQHVLETLGFDITSLGLPDSLRDSI
                     DSRGWIFPRISPAGYSGLMTEQLDGNFSDIHTFYGSVSRTMGSHFLRVGADFRSYLVN
                     NYALGASNGNYTFGSYLSGPLDNSPAPPRGTGIAGLLLGVPDGGGVDVNDSYAARSHF
                     GSVFLQDDWKVTRKLTVNLGLRYEYDGPIAERFNRSVRSFDFAAASPIEPAVVANYAR
                     SPIDEIPASQFRVKGGLTFAGVNGQPAELWQAPRRNLMPRIGFAYMLGSQTVLRAGYG
                     VFFDQIGIVSQSPIQIGYSLTTALVPSLDNGVTFRATLANPFPDGLQKPVGARDGLAT
                     FLGRGVSFFVPDPRTPYNQRWSFGFQRQFLSNFVLDMNYVGSRGTALQISRQLDSVPN
                     EYLSKLRVRDQATIDRLSQNVANPFYPLLPGTGLAGDTVSRSQLLSPYPQFTGVTTQT
                     NDGYSWYHSLQTRVEKRFDRGYTIMGSWTWSKNMEAVSFLNPMDARPERVISPNDRTH
                     RLVINGIYELPFGMGRRYAAGARGVVGKLIEGWQFDAIYQIQSGDPLGLGDFLFYGDP
                     DNIVLSGGERRPERWFNTDGFERDTRRALASNIRYQSSRFSSLRSDGLNYLDFSLIKK
                     TKITERIRLDIRSEFINALNHTVFEAPDTNPYSANFGVVTAGKQLPRTIQFGTVVRF"
     gene            34303..36429
                     /locus_tag="VFQ79_12940"
     CDS             34303..36429
                     /locus_tag="VFQ79_12940"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0140619.1"
                     /translation="MISRSLVLLLASALELAAQIPARWLTEPVLPEATRRGQMYAYVN
                     ANIPPLPQFRSLPEWERYRVTVKRDLLRLLGIDDILRTHRLQVVKKGTLQRKGYRIDK
                     IVYESYPGMYVPALVWRPDRVGAKAPAIVSITGHTYCDSKAADYIQARNYNLVRRGFI
                     VVSYDYFGCGERGRLDACQPGVWGGVDHTNSLFSYTTRTPTGIEVLDGIRAVDYLYSL
                     PEVDRSRIAFTGESGGGNNTYWVAALDERVTAAVTVCAAGSMAQWIKADLNYDWHQRP
                     PGMRAFADIGTLYALTAPRPLLVLNGHKDLAEFAWPDAERSVDYARAVYRLYGAADRI
                     ALERSTTDHGYQLDKRLRLYDWLGRWYFAGKTPGGAAEIDAPVEPREALRVGLSEGLS
                     IPELAKRWMDETRQEVPLPANANDARTFQEDRRQSLAKLLGLRQWGEKPGVAERQTDV
                     VAEGDQEAERLIFEMEPDLLVPAVFVRPVGRKRYPVVIVLEKKRGSSAEVRELLRGGA
                     AVLLLDPRGAGEMDWGGGRTSNWANLTGRTAAGMWAEDVSRVTTYLLARPDVERVAVL
                     GYGIFGKAALYAAALDSRISAAALAMDTISYRAEAEAGFAHVYADVPRILAWGDTPQI
                     AALVAPRALAILRAGEPVSSNQERLSYFSPMPRFDAGPPWVAEAELRDAYSWTQKFYA
                     VFGAASRLRLGASGELETAQWLVAGP"
     gene            36408..37853
                     /locus_tag="VFQ79_12945"
     CDS             36408..37853
                     /locus_tag="VFQ79_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0140620.1"
                     /translation="MASGWPMTTKRLQGSKEGMTRRAVLGLLAAPAAVEASQAVGTKK
                     KVIVAGAGLAGLCCAYELRKRGHDAVVLEAAGRAGGHVLTLRDRFADGLYADAGAEHF
                     YRPGYDQLWSYIDEFRLPVIAYPRRHRVLRLVEGRLLGPEELESPARLRAAGYNEREL
                     RYIGEHSLPGLPGLYFDPYIGRFEDEYKPFAAGLNHLDAMTGREFLLQQGASPAAAGS
                     LGGRRSALQVVWHAAIRRKRGMKWLETNLFRIEGGNQRLTDAFAVRLSGCLRLGCPIT
                     SIEHSPQGVRVHYSDNGRIKQEEADHLVSCMPLINLRKIPLTPGWPETKQYVIQNLPY
                     DSHCRVIFQSRTRFWKSDRVSPNMLLPGPSLGSVWSMAEEVPTARGILIGTAGMTTAD
                     KAVAAYRRAYPGPSEDIEESVVVNWPNDPWAMACLPTGLPPGELTKYWPAMIEPLGRI
                     HFAGVYADNYPFGMEAAVRSARRAAGEITSV"
     gene            37873..39735
                     /locus_tag="VFQ79_12950"
     CDS             37873..39735
                     /locus_tag="VFQ79_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140621.1"
                     /translation="MRKVWIALLLGLSTAPVSLWAQGAVETQRARIRRYPAVWPAGPA
                     PGAIPSWAGPGKIRFSRWDGGRIETAKAFLSGWPGLNPPNPDYLYTMTNWYDPKTVQF
                     AKQAGLNLVWVTLSVGFSDETEAAHREEVRRYIDACHREGIRVMAYESIGNMFWEDMF
                     EKVPESKTWVQLDSNGKPVPYSAATFKMMGRITRYMANLSHPAWRALLMKRIDLAIES
                     GADGIMYDNNFGATLVELYQQIMRHALSRKKDFLVMANFHKDTYVLNRLLNCITTEDS
                     IEPGLYSTASEGYGDLKPHYPYHLAVGDKKLINNFGLMRIHETLSEGWKPVMIEASRR
                     EHQERMVGFMSPQRSQLAMAEAMMFGVGYEHYVEGRPAHLLSIGDPEAVATWQAIGKY
                     NRFFAGHEDLYVGARSKAPLAVILDDESAGVELFDGLAARGVPFEVLYEKDVDRAALS
                     RFKAIALLTARSVRAPALPAMEEFVQKGGRIVAAGDAATLDENGAPRARPSFLASGNK
                     VIIYDRLPGVDQLAATLREVSGEPPVTLDGPPAVLWNLTVQPHQKRTLVHILNYTLQP
                     IRNVRLKVQGAYSGARLISPDGDQTLSAPTLGPSAASSAIPELKIYSVVALDWK"
     gene            39762..40679
                     /locus_tag="VFQ79_12955"
     CDS             39762..40679
                     /locus_tag="VFQ79_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0140622.1"
                     /translation="MRIGITYLYTIFKYGYPHTVDDAIQSLSEIQELGFHYVELEGLG
                     RENLIAVHARRKELRSVLDDCGLHVHNFCVVDPDMVHLDVAVRQPALDRFRLGAEIAE
                     VLEAETLHLASYAPPVEYLQAAPYQLSEGGSYRFEDVTRLRLPQGFCWDAVWNALVES
                     AGFCADVAAEYGRTVLMEPRVGEVICSVDSLRRLIEHAGRPNLKANFDTGHFSAQREN
                     VVLALAKLEGHFANVHVADNHPANAEHIPIGDGSIDWREFLSTLKRTGYDGYLGLDLG
                     LSDQLLDGYRRSVEHLRSIGAELNIEIEV"
     gene            40871..41530
                     /locus_tag="VFQ79_12960"
     CDS             40871..41530
                     /locus_tag="VFQ79_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU0140623.1"
                     /translation="MIIRLIASIILLCGSAALAQPAEAPVSTDQALAKLLQGNQRYVR
                     HKEQHPHKSLTRRKSLVGGQHPFAVILGCSDSRVPPELLFDQGLGDLFVIRVAGNIVD
                     DVVLGSIEYAVEHLGTKLVVVLGHEKCGAVSAAVAGGHDEGHLKALVTAIEPSVSATV
                     NLPGDRVHLCVIENARHVARQIRESQPLLREAVRRNALKVVAADYVLETGKVILLEEH
                     H"
     gene            41686..42015
                     /locus_tag="VFQ79_12965"
     CDS             41686..42015
                     /locus_tag="VFQ79_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140624.1"
                     /translation="MVPGHDPTGPQTVQRWFNTAAFAAPAYGYYGNAGRNILRGPGMV
                     KWDVSLLKSFRVRESLAFQLRGEAYNVLNRTNLSGVSAALGSANYGQVTSARDPRSIQ
                     IGLKMEF"
     gene            42026..43957
                     /locus_tag="VFQ79_12970"
     CDS             42026..43957
                     /locus_tag="VFQ79_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0140625.1"
                     /translation="MKMKKNYSGRVGLALLVLVSPAAFAGEYHVSVNGHDSNDGSSSK
                     PFRTISAAARVAQPGDVITVRAGTYRERVTPPRGGTSGSRRIVYQAAPGEKAVIKGSE
                     VIRNWKPFLPGVWKAVVPNSLFGKYNPYKDLIVGDWFNNKGRPHHTGEVYLNGKSLFE
                     THLLERVLHPVAFADTRDREGSTYTWFAETDDQYTYIYANFHSADPNKELVEINVRDS
                     CFYPNQPGRNYITVRGFHMAHAATQWAAPTAEQIGLIGTHWSKGWIIENNVISDSKCS
                     GITLGKDRATGHNVWINDPRKDGATHYNETIVRALETAGWSRENIGSHIVRNNTIFNS
                     GQTGICGSMGAVFSIIENNHIYNIWTKRQFSGAEIAGIKLHGAIDVVIRNNRIHNAGR
                     GLWMDWMAQGTRITRNLLYDNTTDDLFVEVDHGPFLVDNNIMLSPLSLLDMSQGGAYV
                     HNLLAGRIHSRPEPRRSTPYHKAHATALAGLSSIAGGDNRFYNNILFGNGGPPAAGSE
                     ADANPLRFGGYGLWVYNHREYPLFTGGNVYVEGARPYCNENGDATVEGVRPALAEEGD
                     SVYLRFSLSAGTQKAATRVVNTEMLGKAKIPGLAYENADGSPLVVDCDYFGKKRTLAA
                     PSAGPFDKPGQGDLKLKVW"
     gene            43962..45326
                     /locus_tag="VFQ79_12975"
     CDS             43962..45326
                     /locus_tag="VFQ79_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEU0140626.1"
                     /translation="MATQTDGIQLAGREAATRLYSYRIASVTALSGLLFGFDTAIING
                     AIVFLRRHFRWSEIETEVAAGSLLAGCALGAGVAGTLSDRFGRKVVLLLAALIFALSS
                     VATALPNGLAQFVAARVVAGIAIGIASMLAPLYIAEVSPAAIRGRLVSMNQLAIVLGI
                     LVSYLVGWALAGLGDESWRWMFAVAALPSLLFFAALFFVPESPRWLVKEGRHDEAARV
                     LARLGEPPARLGEIERAIAEESGSLRQLLEKGLRRPLAIGVTLAVLQQVTGINTVLYY
                     GSIIFTEQAGAASTSAALLANVLIGAMNLVLTVVALFTIDRLGRRTLLITAAAGMGAA
                     LTALGFVFAAGSVTPTPVLTLILCYVAFFSFGMGPGVWVVISELFPTRIRGRAMSLAT
                     VALWLACLLITLTFLSLVKAFTAAGAFWIYAFLCAATVWFVWKYTPETKGKNLEEIEQ
                     QWKS"
     gene            45314..45700
                     /locus_tag="VFQ79_12980"
     CDS             45314..45700
                     /locus_tag="VFQ79_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140627.1"
                     /translation="MEIVSPTAVAADTSARRLAAALRHERPDRIPIDLGSTAVTGVHV
                     TCVAALRDYYGLEKRPVKVHEPYQMLGWIDGDLAEVLGVDTAGVFSRETCSASRMKTG
                     GSGIFAASTSWCRGSSTSSKRRTGTS"
     gene            45652..46593
                     /locus_tag="VFQ79_12985"
     CDS             45652..46593
                     /locus_tag="VFQ79_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0140628.1"
                     /translation="MPGKFHVIEEANGDILIYPKGDRSAAPSGRMPVGSAFFDTIIRQ
                     PPFDENNPDPADNLEEFGPVTEADLNHLERSIAWAASTGRGIVATFGGTAFGDIALVP
                     GPFMTNPKGIRDITEWYVSTRSRRPYIHQIFERQCEIALANLARIRERMGDAVQAVFV
                     CGTDFGTQTSAFCSDATFRELWLPYYRRVNDWIHAHTPWKTFKHSCGSVVRFLDSFVD
                     AGFDILNPVQCSAAGMDPEMLKAKYGDRLTFWGGGVDTQKTLPFGSPDEVREEVLRRC
                     EIFASGGGFVFNTIHNIQYGTPVRNIVAMLDAVREFR"
     gene            complement(46785..48194)
                     /locus_tag="VFQ79_12990"
     CDS             complement(46785..48194)
                     /locus_tag="VFQ79_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140629.1"
                     /translation="MMADLPIVPSATRRAFLRACAGCAAACGSSALCAAPPAPESPRT
                     NIRVVFTQAPADKPGWPYVNFDYEAKKKEILAQLKPAGSFAVFETATAMSAEESKKIL
                     ESSASTDGYVVFLLGIPSGGAGREIAFSGRPAIIVDHLYGGTGDFLGTYGPARRKGLP
                     VAGVTSSRFSDVIQALRALHAVKQMKGEKLLDVTGRDPSALTAQIREAYGTEVVKVSG
                     DELNAAYDKASPAEAAEWARTWTKDAGKIVEPTPDEILQGGRMYVAMRNLLAAQRSRA
                     ITVDCLHLFYANKLRAYPCLGFFQLNNENRVGACEADLTSASTMLLMSHLIGQPGYIS
                     DPVIDTARNRIIYAHCVAPSKVWGPGKASNEYHIRSHSEDRKGAAVRSLMPVGVMTTT
                     LEFLPGRREVVMHQAKAMENIDDDKACRTKLAAEPRDAFKLAEDWDAGWHRVTYYGDH
                     RAAIDTAAALLQFKVKIEG"
     gene            48270..49382
                     /locus_tag="VFQ79_12995"
     CDS             48270..49382
                     /locus_tag="VFQ79_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0140630.1"
                     /translation="MKLSAAILLSLGALAQPPPPVMSPEAHADGRVTFRFRAPNAKDV
                     LLARDGASRVPMRQDEQGVWSITTEALEPDFYGYTFVADGVSLLDPMNPRMKPNLLNT
                     QSVVHVPGPASLVWELGSSAPRGTVHRHFYRSAVVGDDRDFYVYTPPGYDPTAHRTYP
                     VLYLLHGFSDDASGWTAVGRANVILDNLIAQRKAKPMLIVMPLGYGAPEIVSRTPGPG
                     RAGLRQRSFDKFREALFTEVIPEVEKSYRVAKDRTARAIAGLSMGGAESLYTGLNAVD
                     RFAWIGAFSSGGMGDDLEKSFAHLDAKAGAQLRLLWIACGKEDRLIESNRKLLDFLQS
                     RGVRYTWRETPGAHTWLVWRRYLAEFAPLLFQDKTS"
     gene            complement(49921..50577)
                     /locus_tag="VFQ79_13000"
     CDS             complement(49921..50577)
                     /locus_tag="VFQ79_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0140631.1"
                     /translation="MTGRESARIDRAMGSALGTGKHKAPALVSEWLEALNWAEIQRVH
                     EEADPRLSPVALVYADIADKNKPLFSPAFLRREFFPRLRSLVAAWHAHGIKVIFHSDG
                     NLWPVLDDFRASGIDGLNPLEPLSGMSVEAVAPACPNWILMGGIDATQLLPFGREDQV
                     RAAVRRAIAGAGGCGRLWLGSSTEIHPAVKVENVLAMWDEIERCGYYEFATALRNVAV
                     "
     gene            complement(51488..52339)
                     /locus_tag="VFQ79_13005"
     CDS             complement(51488..52339)
                     /locus_tag="VFQ79_13005"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0140632.1"
                     /translation="MIVVDAHQHYWDTAAVRNERLERIPELRGRFLPEQLASIAPPDI
                     AASVCVEAASAGADGRSETEWLLRAIRTARIPTRIVAWAPVGTEAAAGYIEWLRNVLG
                     LVLAGIRQGFEHGAADLICSKQVVKGVRSVGAAGLPFDVVVRQEMLPHVRVLAGNCPE
                     TTFVLNHMGKPRVSGTPSTSWCRDLEALAANSNVLCKISGLMTEADSTVLNPQTYSQY
                     IQHAIDVFGWDRIMFGSDWPVCTLAASYGQWLNIVERSVSKESPSNQDKLFASNAIRV
                     YFGGSLA"
     gene            complement(52349..53689)
                     /locus_tag="VFQ79_13010"
     CDS             complement(52349..53689)
                     /locus_tag="VFQ79_13010"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0140633.1"
                     /translation="MSNTDRAHPTSRTLDQRALRTVPGGVHSSTRRTNPPIVFKRASG
                     SRVWDVEGKEYVDYHAAFGPIILGHCDADVDRSVAEATREIDLMGLGTTEPEIEAAET
                     ISRHVPSAEMVHFCNSGSEATYNAVRLARAVTGRKKLVKFQGCYHGWHDYLCMNVISP
                     RDRIGTYDPGSAGMLPEALENTIVLTFNSVPELEQTFVDFSNEIAAVILEPIPHNIGC
                     VMPRPEFLDALRDLTKRNGSLLIFDEVITGFRHALGGYQSICGITPDLTTLAKSMANG
                     YPCAALCGKRDYMQRFATAGGDVFFAGTYNAHPAGIAAALATMRKLETSGFYKHTARL
                     AERLKRGLQALVDQFGIRAWATGFGSVVVLYFKEPGADNYTELLDCDVAADVAFRRRL
                     LEQGSLVLPLAYKRLHVSGAHTDDDIDRLLNHAEDALKSLAAGTPAVGLTARGH"
     gene            complement(53699..54544)
                     /locus_tag="VFQ79_13015"
     CDS             complement(53699..54544)
                     /locus_tag="VFQ79_13015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0140634.1"
                     /translation="MRVDYHTHFMQPAHFGPDFIKEWAARGGTGAWPEISAEQFESAV
                     KQFDRVIVFGITALALGIHTPHEYVARLCERNRRKYIGFMALDPSSPGAIEEMERGVR
                     DFGMKGIKLYPVMSLYNPPDPALHPFFQRATELKLPILTHTGTSPASKGLLRYSLPLQ
                     IDELAIVFPDLRIIMAHLGHPWQRDAAMVIRKHPNVYADISGIWHRPWQGYEAMIVCL
                     EWGVTDKLLFGSDYPLWDPAQAITALRQLNRYAEGTNLPRIPGHVFDEILNRNILSEL
                     GITTD"
BASE COUNT        10329 a        16862 c        16994 g        10471 t
ORIGIN      
        1 tagtttcatg gtacctgacc tggattcaga aggctcatgc tgtctccatc tccgtcgttc
       61 gcaacgggac acccagcgcc tccagggtcc ctgaggcgtg cggaatccga gcaagcctta
      121 aaccaacgaa aggctgccct caaggactat tcggaagcca ttcgttttca tcctggagat
      181 tggtttatat acatgcgccg agctcgtctt cacaacgcta tgcgggcttg ccgatcggcg
      241 attgcagacc tcgagcagat cttaatcctg cgagcggact ggtctgagcc ttacgcgttg
      301 ggagccagca tccttgcagg ttgccctgac agcaatttac gggatccgaa gaaggcgatc
      361 gcatgggcgg aacgcgcgat ctcactcgac aagaacaggc ctacccttct gacgatcctt
      421 gctctcgcat acgcttcgga cggacaactg gataaggcgg ttgcagcaca gaggcgcgct
      481 ctgaactccc cgaacttccc gatgccctat cgcgagaacg cagaacacca actgcacaag
      541 tatgaagccg atctgagccg ccaaggcaag tgatccgggc gatgtcacct atcttcgcgg
      601 ccgaaagccg ccggtagcgg ccatgtggcg ggtaatcccg ctgcgatcgg acagcaagcg
      661 agattggcaa aacgctccgc cgttgaatcg cgcgcgatgt ccgcgacggc aactcctcgg
      721 agtcttgacc tgcgaattca acaagccgta cacgagggca agtgcctccg catagcgatg
      781 gcactcgccg ggcaaatggc actccattct cgcgagtgcg atatcgcaac aggagccgct
      841 gaacggtcga tctgcacgaa gccgtgacgc ttccagtact cagccgcggt gtccgtcaag
      901 aggtacgccg ttgtgcagcc gcgcacgctc gcatgttcga ggcggtccag tgagagacgg
      961 tgaccgattc cgaccgaccg ccacttctcg tcaacagtca cggaccggag aaggatgtcc
     1021 gtgccatacc tctcgtaccc ggccataccg acaatggttc cgtccactgc ggcggccacc
     1081 gcatactgat ccccgaactg cgccgtatcg tcgatgggga cgaggcgggc tgaacgtagc
     1141 agttcagaca ccgcctccaa gtcggctgct gtggcaggac ggatcagaat ctctgaaggc
     1201 atcacttcgt tccggagcag cagctcggac cgcagcacgc ggccggcttt gttgcccgca
     1261 cgaacgcgct cgcgaacttc ccgtcaatct gcggagcgat tgcctcgaca tccaactccc
     1321 gcccgctgaa gagctggcgc gcgtcatcgg cggtgtagac gcgagtcggt tcgatctcca
     1381 cggactggaa cccggcagcc ttcaccttgc tgatgtagtc cgtgtcacga agcgctcccg
     1441 caatgcagcc gacccagagt tccacgtttc tctggacctc tgcaggcatg tcgccccgaa
     1501 caatgatgtc ggacaccgcc agcctcccgc ccggcttcaa cacacggaac gcctcgtgga
     1561 agaccttgtc cttgtcagcc gagagattga tgacgcagtt cgaaatgatg acgtccacgg
     1621 agttgtcggg cagtgggatg ttctcgatct cgcccttcag gaattccacg ttctcgatgc
     1681 cggccttctt ctgattctcg cgcgcgagag cgagcatctc gtccgtcata tcgagaccgt
     1741 atgcctttcc tgccaggccg acgcgccgcg cggacaggag gacatcgatg ccgcctccgg
     1801 agcccaggtc gagaacagtc tcgcctgggt tcaacttggc gagcgctgtc ggattaccgc
     1861 atcccaagga ggccaacagc gcttcttgcg gcacgttggc gccttcctca gcgctgtaga
     1921 tctgcgaggt gatcgggtcg caactggcgc cgagcgccga accgccgccg caacaggagg
     1981 ttttgcccga ctgcacccga agggcggcct ggccatattt ctctttcacg acttctttga
     2041 tatctgtgct ggtgttcatc tgcttcctct ttcgccgggg cgaatccgtc atacggacac
     2101 actccggacc cggtgttcgt ttcgaattga ttcctggtcc cggtctacct ggagatctga
     2161 acgatcgcct ccggttcgac cgcttggtta cgtgtacagc actcggcata aaggaagccg
     2221 agaaggtctt gcagcgcctc ggcgttcgca gcgtagcgta agaacgtgct ctcacgcttc
     2281 accgtcacga ggccttcgtt cttcagcttg tcgagatggt gcgacaacgt cgagcctgga
     2341 atctccagcc catcgctgat gtcgcctaca accatcccac gcggatgggc ggacagcagg
     2401 aggcgcatga tcctgagccg cggttcggct cccatcgccg ccatcatatc cgcgtaccgg
     2461 ctcacgtctt gcggactggt ccggcccttc gctcctgagt tgttttctaa agccggccca
     2521 cgtgtccctg ccacatttcg ataatagctg aaatatagaa tgagtcaaga ctttttttcg
     2581 agtgatcacg tccaggcttc gattacgtac gagccgcggt tgagccagaa gtaacgccaa
     2641 ctgaaccggt ggaacaccag ataacgaagg cgctgcttga ctcgaaccaa attcgcatga
     2701 tatcgttaat cgacgataga ctatgaaaat aaaagacgat ccgatctgtt gcccgccggt
     2761 ggaaccgcag gtgtacggta cccttcccgg cctcgagggt ccggatgcgg acgaggagtt
     2821 ggcaaagctc gcgaaagcca tcggccatcc cgctcgcgtg cggatcctgc gcatgttgtc
     2881 gcagaaggaa gcccgcgttt gcagccagat tgttgacgag ttgccgttgg cgcagtcgac
     2941 cgtctcggag caccttcgga ttttgaagga tgccggtttg gtgcgcagca gccaggacgg
     3001 gccccgaatc gggtactgca tcaattatgg aacgctccga aaactgaagg ctttagtagc
     3061 catcatctga ttcgcctgga aaccaggata tcgccattca tcgatggcgg aggaaaggtc
     3121 atgaagaaag tcgaagtttt cgatccccca atgtgctgct cgactggcgt ctgcggacct
     3181 aacgtagatc caacgctggt tcgattcacg gctgatctcc attggttggc aaaccagcgg
     3241 gtggcggtcg aacgatcaca ggaaaaggcg gtgtcggcaa gacatcactc gcctgggcgg
     3301 cagcagtctc gttagcgcag agaggcaaga aggtattgct ggtcagcacg gcggctcatg
     3361 tagcgacaac gatcggtggc gcggctccca acctgtccgt gagcgggatc aatcctgccc
     3421 gggagacccg ccaatacacc gaagcggtga tggccaaatc ggcgccgcac ctggacgccc
     3481 aaggaatggc ccttctggaa gaggacctgc gctctccatg cacggaggag atcgccgttt
     3541 tcagcgcctt cgcgcgcact gtagccgaag ggtcgggccc gaccggctca gagaactggt
     3601 gagtagttca ctaacaggcg cggaagcgcc gaaaggctga atagaatgga gaatcgatcc
     3661 accccccggc tttcctttct cgaccgttat ctgactctgt ggatctttgc cgcaatggct
     3721 cttggcgtcg gactgggtta tctggttcct ggcgtcgtgc cgttcctgaa ccggttcagt
     3781 gtcgggacga cgtcgattcc aattgccatc ggactgatcc tcatgatgta cccgccgctg
     3841 gcgaaggtga ggtacgaaga aatgggccgc gtcttccagc accgccgcgt tctgctgctg
     3901 tcgctgatcc agaactgggt gattgggccg atcctgatgt tcgcgctggc ggtgctgctc
     3961 ctccgtgaca agcccgagta tatgacgggc ctgattctga tcgggctggc gcgctgcatc
     4021 gcgatggtga ttgtctggaa cgacctggca cgcggcgacc gtgagtatgc tgccggcctg
     4081 gttgcgttca actcagtttt ccaggtgctg ttcttctcgg tgtattcgta cttcttcctg
     4141 gctacggttc cgcgctggct gggtctcacc gcggtggaag tggacatctc tatgggcgag
     4201 atcgccaaga gtgtgttcat ctaccttggg attccgttta ttgccggcat gctcacgcgc
     4261 ttcgtgatgc tgaaagctaa gggccgcgag tggtacgaga agagcttcat cccgaagatc
     4321 agcccaatca cgctgatctc gttgctcttc accatcgtgg tgatgttcag cctgaagggt
     4381 gagatgatcg tgcaactacc cttcgatgta gtccgcgtcg ccattcccct cacgatctac
     4441 ttcgtactga tgttctttgc ctcgttctgg atgagcaaga aggtaggcgc caactatcct
     4501 gaggccacca cgctttcgtt cacggcttcc tcaaacaact tcgaactggc catcgctgtg
     4561 acgatcgcga cattcggaat tcaccacggc gccgcattcg cggccgtgat cgggccattg
     4621 gtggaggtgc cagtactgat ttcgctagtg aacgtggcgc tctggatcaa tcgcaaatgg
     4681 ttcgggggtg tatttgcaag tgttgatacg tgccaaacgc agcgagcgta gtcgggcatg
     4741 gaggagaaga agaacacatg aagaaagtgc ttgtcctttg caccgggaac tcggcaagga
     4801 gccagatggg cgagggactg tttcgacatg agggggctgg ccggtatgag gtcgagagcg
     4861 cgggcacgaa gccgggcgac gtccggccgg aggcgatcgc agctatgaag gaaattggaa
     4921 tcgacatatc ggaccaccga tcaaaatccg tcaacgaatt ctccggtcag tccttcgact
     4981 acgttgtgac ggtttgcgac aacgcccgcg acaactgtcc ggtgtttccc ggaaaggcgg
     5041 agcggatcca ctggagcttt gaagatccca cggctgttga gggtgatgaa gaggtacgcc
     5101 ttgctgcctt ccgccggatt cgcaatcaga tccatgaacg cgtgaaggcc ttcgttcgca
     5161 agcgggcggc gctggaccgg tagacctcac tggcgagtgc gggttgtgag aaagcgagta
     5221 ctcgtagttt gctcgggtca ttcagcccgg ggtgggcata gaccgtctgc gacagggcgg
     5281 cgcgggaaga atgtcctgcg ttttccggtg agcccgagag accccattgg ccgttcgagg
     5341 acccggcaat tctggaaagc cagtggcaat acgccatcgc actcagacgc gtgaagagcg
     5401 gcggcgccgc gtcgtcggaa tcggatcacc cggagagatc accttggcca ccagtttctt
     5461 ttgagggaag tcttttcggt tcaaggtgac aatgaaggcg gcccgtccct cctcagcgca
     5521 cgcgcagaat gcgttgtcgg ctggatcggg cgatacgtcg cttccggagc ggacctccac
     5581 gaactctgcc tcctccctga ggagattgat aatttcgccc accaggctcc gccgaacaac
     5641 gagtctggtg agaactccct tgtattccga aacaatctct tccgtaagca gccaaatgaa
     5701 ggtcccatct tcaacccagt cccgaataag ggctgcgctc ggattctcta cggtgcgctg
     5761 ggacttaaat cctgcgatgc cagcgatgag caccgatgta tcgacgaccc ctctaggctg
     5821 ttttcctcga gggcgcaacc gtaatctccg gcaaccgctt cttctccgcg cgaaaacccc
     5881 gtattgcctc cgcgatctgg gtctcggtta cgccggacag ttcatccgcc gccgccgcca
     5941 aactgcgtgt gaactcacgc ttccatcccg gtctctttcg tcctcgcggc aaatccttgt
     6001 acacggctgc cagaggaagc tgctcctcca gttcttttgc gagatctctg acgggaattc
     6061 ggacctcaat cggctctcgt cccctggacg ggcgcgaccg cagcgaaagg tagcgaacgc
     6121 tcgtcatatc ctcgtcctta agagcggaca gagcacgcga gaaaagggcg aggacctctg
     6181 tggatatgcc cagggactcc ttcaagtaca atgcaacttt cgcctccaga agaagtcgat
     6241 tatcgaacca gtagaagctg tcacggcggg ctgggcgcaa gatttttaat tgcacaaggt
     6301 tctgcagatc ttttggggtg acgcccaata ctgcactggc ctgcttaagc ttcaactcaa
     6361 cgccttccat atctctatac agtctatatt ttagacggca attctttcct ggtcaactgg
     6421 gctcctgtgc cttcgttgga cggtttagca gctgtatgct tttcgaaatc aggctcccgg
     6481 cggacaagct gaacggcgac atccagatca ccaatccgtt ccgtttcgcc gatgtagctc
     6541 ccgaatacta ccgctttcta aactcgcgcc aggaagtgag gattacgatt caaggctgtg
     6601 atgcgctcga gcaattccga cagtaggcgg ttggcggttt cgcgccatgg cggtttggcc
     6661 gccgtcgctc ctcgcaggtg aatccatccc tggacaacta ttgcgccgtt cactctcccc
     6721 cgagccccga cctgctccag gaaacccatt cgccggagca cagtgatcag gcgatcggtc
     6781 ttcgactcga aaggttcaat tgccgccgca cgaattcggc cggcacaacg ccgtccagtc
     6841 ccgctcgccg aaaccaattt cacaagtcca tcggtggtga tttaggggct gcccggcctc
     6901 ccgattcggc cagcaacgaa tctgcctcaa ccatgtccgg gaaggccggt ccgtcgatgg
     6961 cccggcgcgc ttcggctact attagtccgg cctcggcgcg gcgcccgcgg cggcaccaca
     7021 gccgtcccag actgactgct gcacgcaaca ccagcaactt cgcgccttga ctccgcgcaa
     7081 tctctatcgc ccggaggaat acctgctccg cctcgtgttt tgccgaggaa gcactcgcgg
     7141 ccaatagaag ctcccctttc agcctgtgca gctccgcctc ccagaagcgt tcgcccgtgc
     7201 gcccggagac ggcaaatgcc tcgtcgagcg cctgcaagcc cgcgaccgcc tgtccgcctt
     7261 tcagcagcgc gtccgcgtgc agtcccagta aatacgtcat ccacgaattc gacccggtcg
     7321 cacgaacttc agccaaaccc cgagagatga ggtcgaggcc ttcacggtat tgctggtgct
     7381 caattgctgc ccatccttca aggggccagg cccaaccgag aatgagccgg aaatcctgct
     7441 cgcgggcggt cgctacagcc gcctctgcgt gtattctcac tgcgtcgctg tcgcgctgca
     7501 tctggccaac agccgctgtg aatacctgag ccaacgccag ggacatcggg tggctcaact
     7561 cttgagccaa agcgatgcac tgattgcagg cgcgagccgc ctcgtccatc cgcgcctgca
     7621 atgccagggt cagaccgagg aaatactggc cacagacagc ggcgtcgtgg cttccatagg
     7681 ttgcggccat agccgcatgc cgctcaactt ggtagatgcc gatggctgac gtcgaatgga
     7741 tgcgcgcggc atcgagatct ccccgtaaga aagccgtagc ccaacctgag tgatgggcct
     7801 ggaggagcaa ctggcggtcg tcgtgccggc gggcaagggt catcaggtcc tgcgcgaggt
     7861 tatgcgcaat gctcaaaggt ccttgtccca ggtaatagag ccaaaggccc caaagggcgg
     7921 ggaagatccg cggagtgtcc ccgagtccgt gacacaactc atacgcccgc gagtatggat
     7981 gctcgacctc cggtgcaccc cagccgtgag tcgccatcaa caccgctccc agcccaattt
     8041 gcaacgccac ttcatgctcg gtccgctcct ggccgggtgg ctcgtttgcc agcagcataa
     8101 gcgccttctt gaagtgcaac tgggcttcct tgtaggcgtt gcggcggcgc gcgttctccg
     8161 ccgcgtgctg acgatagatt ccagcacggt gcaggtcgcg gctccgttcg aaatgcatag
     8221 ccagctccgc ggcgatttcc ggcgaccgtt gtccgtatgc ggtctcctga cgttctccaa
     8281 ttttccagtg caggtcagcg cggcgactcg ccggaatacg ttggtacaac acgtctcggt
     8341 acagcacatg caggaagtcg aagccggcac tgatggttcc gtcgggccac tcgaccgcga
     8401 cgccttcctg cacgaagcga cgctggcgag cgagtgcggt cagcgtgatt tccacttcat
     8461 ccagctcgcg ccccgcagcg accgcgaccg cggctgccga gaatgtcgct ccggcaccgc
     8521 tcgcgatctc tagaatgaag cgttcatccg ggagcaggcg attcacctga cgctcaatcg
     8581 cccttctcaa accgtcgggg atgcccagag cgggctcgcc tgcatcacca ctcaccgtcc
     8641 atctgccatc ctgctcgagc aataggccac gttcgaccag atcgcccaga atgttgacga
     8701 cgaacagcgg gtttccgccg gtgtgctgtt gcacgactcg agccagttgc ctcatgcgtt
     8761 cgttatggcc ggtcgctggc gggaaccgaa gggcaacata gtcggcgact ccgccttcgt
     8821 ccaggccccc cagcgcaatc tcccggctga gacctttttc ccggagccgc tcaaccacct
     8881 cggccagcgg atgctcagtt ccgctgaccg ccggaggcca gaacgtggca atcagcagaa
     8941 ggcgggcggg ctcgggccgt tgcgcgaagg cgacaaccca atcgagcgtg gacatgtcgc
     9001 tccaatggag gtcttccaac cagaggatca gcggcgcttc cgcggtgatc gcttccacgg
     9061 cgtcgttgag ttcccggagc atgcgctctc gcgtcgtgcc ggcggctgta cgttgcagac
     9121 taaccaggtg ctccggcgca agtaaggccg gtatttgcgc cagccaggtc gggctgtatc
     9181 gctcaagcag gtggacgatc cggtcgcccc cgggctgctg gcacagcctc gtcagcgctt
     9241 ccagcaatgg ttgatacggc tctccaacac cgtagtgctc gatactctga ccccaggtcg
     9301 ccctgacatt atcgcgggac ctcgccctcg ccaggaaggc tcgaacgacg gttgtcttcc
     9361 caataccagg ttcaccggtc acaaagagca cttgccggga gccatgttcg gcccgggtga
     9421 atgcgtcgag catgcccgca aataccagtt cacggccaat cagcggcgca tcggtgtgga
     9481 tcggcgccgg cggcccgacg tttgggcccc cgtccggcga accggtcagg gttggtgcac
     9541 ggaaccggta accccttcga tgaagtgtct cgatgaatcg gggacgccgc gctgtatcac
     9601 cgagtacact ccgcaactcc cggatgcagg acgtcaaagc agagtcggta accgcggtgc
     9661 cgggccagac cgcacgaaat agctcttcct tggtaacgac ttccccggtc cgttcagcca
     9721 gaacacacag cagtgcgagg gacttgggtg taatgcgcac atcgatggaa ccacgccaaa
     9781 gtccctggac gggatcgagt cgatactgat caaagcgaat cacctcgaac tccgaagcgc
     9841 tcccatcagg atcccacgga aatcccagtg ctttcccacg acttcatctc gacacccggc
     9901 ttactttcga ttttacaggg ccgcaagagc ggccacgaaa ggaaagggac ttggatgatc
     9961 agaatgacta tcgcagtccg tgtaatggca gcgatgctgt ttacttcggc gcttacccgc
    10021 gctgacgtgg tctcggactg gaacagggta atgctgacta cactgagcgc ccagaacccg
    10081 tacattcagg cgcgagttgc cgcgataacg cacctcgccg tcttcgaggc cgttaatgct
    10141 gtgaccggcg aagttaagcc gtacctcggc actgtcaccg catcgccggg cgcgtccgct
    10201 gaggccgcgg cggtttctgc cgcgtatacc gtgcttatca aatacctccc cgactgggca
    10261 gatgcgctca acgacaatcg ggccgattct ttggcaagga ttcctgaggg accggccaag
    10321 agcgcgggta tcgccgccgg cgaagctgcg gccaatgcaa tgatcgccgc gcgcgccagc
    10381 gacggatcgg agccgccgga agtttacacg ccgccacctt ctcccaaacc gggcgaatgg
    10441 caattgacgg gggcgtgcaa cggtggagtc tttctgcacg tggcaaaggt caccctattt
    10501 gctctccgca gcagcgacca gtttcgctcc gatcccccgc ccgcgcttgc cagtggtaag
    10561 tacgcgcgtg catataacga gttgaaggag tttggcgggg tcaacagccc tggacgtccg
    10621 caggaccggg ctgacgtggc actcttctat tccaaggtac tgtcaacgtt tatctggaat
    10681 ccggtggcca cccagattgc cgcccgacag aacaagtcgc tatcggaaaa cgcgcgtgcg
    10741 tttgcgctcc tgaacatggc catcttcgat gcctttgttg cgaacttcga tacgaaatac
    10801 cggtacccgt tctggcggcc tgagaccgcg attcatgccg gcgatcaagg cggcaaccgg
    10861 aatactgaac ctgaccctgg ctttgccccg tttgtcccca cgccgtgcca cccgagttat
    10921 ccagcggccc tcccggctct gggctctgcc gcccgcgtcg tgctggagcg catctatggc
    10981 cgcagaggcc actcaatcac gttgtcgacg ccggccgttc cgaacgtgac cctgcattac
    11041 gagaatctta gggacataag cgatgacatc aatgacgcgc gcctcttcgg tggaatccac
    11101 ttccgtttcg atgacgctgc aggcaatgag cagggccgcc aggtgggcga ctacgttttt
    11161 cgacacaagc tccgacgcgc gcagagttgc gcctctggcg aggaggaaga tcaatgatgc
    11221 tgcattgcac aagtgcctta acaatgggaa accggcggcg tccgcttccc gcattcgccg
    11281 caacctggag ccaggccctt ctggctgccg gcacgattac cattttcgcg acctccggac
    11341 tggcccaacg ggccgacttt aacggcgatg ggtttgcaga tttggcgata ggcgcgccca
    11401 gcatcggccg ggagggccgc gtgcgcgtga tctatggctc gttcacaggc ttgaccggca
    11461 cggcgggccc cggtgaccag tcctggcgtc agggtcattc gggacttggc ggtataccgg
    11521 aaccgggcga caacttcggc gcggccctgg cgacgggcga tttcaacaac gacggatacg
    11581 gcgacctagt agttggaacg ccgggcgaag atgacaacag cggtgagttg agggtgttat
    11641 acggctcggc tagaggactg caagttgtcg gcgtcggcgc tcctcccgag aagtggatac
    11701 ggcgcggcct tccaggagtc gagggtacaa gacggttgga cgacagattc ggcgatgtcc
    11761 tggcagcggg cgacttcaat aatgatggct atgacgacat agctgtcggt gcgcccggct
    11821 tctttagcgc caatcctgac aggtacgccg aagggtctgt gagtgtgttt tacggctcgg
    11881 tcacaggaat acaattcaac ggccagggcg gccctgatga ccagttctgg aaacagggcc
    11941 tcaatggtct gcagggagta tctcaaaggc acgatcactt cgggaacgct ctgactgttg
    12001 ccgatttcaa ccgcgatggt tttgcggatt tggccatcgg catcgtcagc aaccctggcg
    12061 gaggagccct gagtgttctg tatggctcgg cggccggatt acaggctacc ggtagcggcg
    12121 cgccggatga ccagtttatc ggccgggaca gtccgggcgt aaagggtcat ggcggagaag
    12181 gcggtttcgg agccgcgctg acagccggtg atttcaacgg cgatggcttt gccgacttag
    12241 ctgtcggcgc gccgttccct tttgaacgca cgggctcgct gaatgtgctc tatggctcgc
    12301 cgtcagggct gcaggcgacc ggcagcgtcg gtccggatga tcaactctgg agcccggaca
    12361 gcgcagacgt cgcaggtaca gggacaccgg gcgaaaagtt cggcgcttct ttgacggccg
    12421 gcgacttcaa ccgggacggc ttcgccgacc tcgccatagg agcgccgtat aagtcccgga
    12481 gcgggcttct ctggtacgga caggtgtacg ttttgtatgg ctctggcgcc ggcatacagg
    12541 ccggcacaaa cggaccggaa gaccaactct gggatcgcgg aagtattgac ttacaaggcc
    12601 ccgccgccgc atcgcaggac ttcttcggtt ttgcgcttgc ggcgggcgac ttcaataatg
    12661 atggctgcgc cgatctggtg attgggaacg gcgccatcgg cgtccaggca aatggcgtcc
    12721 atgtgttgta cggctccccc ggaaacggtc gccaaaacgg agtgcaggct accggtaacg
    12781 gcgcgcctga tgaccagttc tttactccga tcaacgcagg ggaaggcttc ggtagagcgc
    12841 tggccgcgag caattgaccc aggcgggagg ttgccgcatc ggaatgatga aaggacagta
    12901 gaacgatgga gattgccact tctcacggca acccggtcac tattgcggaa gctgacctgg
    12961 acgaccttcg aatcgcgatg cgcgggtctt tgcttcgtcc cggcagcctt ggctacgacg
    13021 aggcgcgcgt cgtcttcaac gggatgttcg accgccatcc cgcgctgatc gcgcggtgtc
    13081 gaggcgtcac ggacgttgtg gatgccgtgc ggtttgcccg cgagtgagat ctcctcacgg
    13141 cggtccgtgg aggcggtcac agcgtagcag gcaactcgac ctgcgacaga ggcttggtga
    13201 tcgacctttc gctgatgaac gcggtcacgg tcgatcttaa gcgccgggtg gcgcgagtcc
    13261 aaggcggagc cacatgggcc gacgtggata gggagacgca agcgttcgga ctcgcaacac
    13321 cgggcggcat cgtctcgcac accggcgttg cagggctgac tctgaacagc gggattggct
    13381 ggttgcggaa caagtacggt cttagttgcg acaacgtggt gtcggcagag gtagtgacgg
    13441 cacgtggcga aattctcacg gcgagcacga gcgaaaacgc cgacctcttc tgggccatcc
    13501 gcggtggcgg cggcagcttc ggagtcgtga cctccttcga gttcgccctg catcctgttg
    13561 ggccggttgg gccggcggtt ggcactgtgt tttccatgta tccgatggca gcgattcgga
    13621 acgtgctgaa acagtggcgc gagtgggtcg cgtccgctcc ggacgaagcc gctaccgaaa
    13681 tcgtggcgtg gacgccgcct ccggcagcga gtttgccgcc cgcggtacac ggccgggagg
    13741 tcgtcatcgc cgccggagtc tgggctggcg atccacagga gggcatgagg ctgctgcaac
    13801 cactccggga attcggtaat ctgctcagtg agatcgctgg agtgacatcc taccgaagtg
    13861 ttcagagcgc cttcgatccg ttctttccga atacgggagc ggtgatgggc tactggaagt
    13921 cgctgtacct tcacgacctc agcgaggccg caatcaaaat tatcgccgac cgcgcgaaaa
    13981 ccgcagttcc ccgtcgacaa tggtgctggt cgaacatctg ggaggcgccc tgcgccgcgt
    14041 gcaccctaac gaaaacacct ttgccactag cgatgccgcg ttcctggtga actttatggg
    14101 cgattggagg gacgcacgtg agacgccccg gcatgtcgcc tgggtccggc aggcgtggaa
    14161 ccggctcggg ccccactcca ccggtactgt ctatctgaac tacctcggct cggaggacgg
    14221 cgaggtggat acactggtgc ggtccgcctt tggctcgaac tacgaccgcc tcgttgagat
    14281 caaagcgaag tacgatccga cgaacttctt ccagctcaac ccgaacatca agcttgtctc
    14341 acggatgaca taggaactcc ggcttcgggc gggatgcgga tttcgtgtgg tattccggtg
    14401 gtggttccat gaaccagggc gcccaggctg cctggtggag cgtttgaaaa taagggaggc
    14461 cactcgaggt gaggactgtt ggaacgattg aaagtttatg gcgttatccg gttaaaagca
    14521 tggccggtga agcactgacc gaagcattta tggggttctc cggcgtttac ggcgaccgct
    14581 gctacgcctt caagagttca gccgcgcgca agggctttcc ctacctgaac gcagctgcac
    14641 agcaacggat gttactccat catccgaagt tccgatatcc ggaaagggca gctaaaccac
    14701 cgaatctgat cgaagcgatg agcattaagc ccggtgtcac cccggcaaat gccgaacctg
    14761 atgacttgat gctggatgtc gtgacgcctt ttggcgcggt ggtcggcatt gatgacgcct
    14821 cgttgacaaa gagtttgcgc gaagggttgc gcggtgaaca tcatctcacg ctcgtgcgtt
    14881 ccgaccgtgc gttgaccgac tgtcgtccga tttcagtgat cagtctgcaa acaatcaggc
    14941 agatcgaaga ggaatcgagg atcgcgattg ataaacgacg gttccgagcg aatgtctatt
    15001 tcaacctcac gtctaatgag agctttgcgg aggatgaatt agtcggtcgc aacctgcgtc
    15061 tcggatcgaa ggcagttatt gcagtcctgg agcgtgatcc gcgatgcaag atgatctctc
    15121 tcgacccgga tacgggtgaa cacaacccgg cggtgcttcg caaagtggct caagcacacg
    15181 aagcgtttgc tggcgtttat tgcgcggttt tagtcgaggg gatcgttgcc gtgggtgatt
    15241 caatcgaggc agttggctag gaggagagtc cgtgagatgc ggctggtcac gaaccgccag
    15301 gctacgtcga atacctggca acggtgtcga gtggtcactg gatttccaaa ccgatgtagg
    15361 ccgtccggat tttcgcggcc tgaagggctg gtgcgcatga agatttctcc tgcatcccgc
    15421 acaccgggca actcccttag caaccttccc ggccccaggg gactgccgct gctgggcaat
    15481 ctgctgcaat tggacgtcac gcagctacac gtcatcctgg aggagtgggc gaacgtgtac
    15541 ggcccgctct atacgttcaa aattggctca agaccggtcg tagccatcgc cgaagccgcc
    15601 ctcattcacg aggtgttgcg tggccggccg gagatgtatc ggcgcctggg ctctatcgcc
    15661 cgcgtcctga cagaggcagg cgtcaacggc gtatttgccg cagaaggtgc gtcctggcgc
    15721 cggcagcgcc ggatggtgac gcaggcgctc gacaacgaac atctacgccg cttctttcct
    15781 acgctcgcca ggataacagg gcgcctgaaa agccgctggg accgcgtggc ttgcaaagct
    15841 tgtgcgactg acgtgcaaca ggacctgatg cgctacaccg tcgatgtcac cacgaacctc
    15901 gtcctcgggt acgacggcaa cacgctggaa aacgaagatg atgtgctcca gcggcacctc
    15961 gaacacctct tgccggcact caatcgccgg atcaatgcgc cggtacccta ctggcaattt
    16021 gtcaggctgc ctgccgaccg ggcctttgat gacgcgctaa gggtggttcg cctgctgatc
    16081 gcggatctcg ttgcccaatg cgaggcgcgc ctgaagcacg cccagggaga gggatatcat
    16141 ccgaccaatc tcttagaggc tatgcttgcc gcgcgagaac aggagggcgc gtttactagg
    16201 gaagagatcg tcgggaatgc cctcaccatg ctggtagccg gcgaggatac gaccgcccac
    16261 accatggcgt ggatgatcca cctcatcacc gggcatcagc aggtccagaa gaggctgcag
    16321 caggaggtag acgaggtttt aggtcaggcc agcatgttgg aggacttcga agttcaggaa
    16381 cgtctaacgt ttcttgaagc ggttgcgcat gagaccatgc gtctgaagtc ggtggctccg
    16441 gttctgtttc ttgagaccaa tcatgccgtg gaactgggtg gtgtgcatat gcctgaagga
    16501 acagcgttat ttcttctcac ccggcagtgc ggtttgcagg agtgcgcgtt tgcgatgccg
    16561 accgagtttc aaccacagcg ctggcttgcc ggcgcggagt tatcgccggg ccaacgtacc
    16621 tcgaacgcgt tcatgccctt cggagatggg ccgcgttttt gtcctgggcg ctccctggct
    16681 ttgcttgaga tcaaagcagt tgtggccatg ctttgccgca acttctgcat ctcgaaagcc
    16741 gaagccgcaa aggaagtcgg tgaacatttt gcctttacca tgatgcccac aaatctcgtg
    16801 gtcactctca ggactcgaga gtagctcccg tatggcccga gtatccagtc tgcgactcag
    16861 atgagcacaa aaccacgcgg ccgctatcaa ggccctggat ttcttgaatt actacttggc
    16921 gtctattaga catgcggtca actcctttcg tgtgcggctc tgcgccgcag atctcatcga
    16981 ttcacagcgt catccgcgtg atcgcgaatc ggagtggtcg ctccattgtg actaataatt
    17041 agaccaatga agattacata ctcttgccgg atacggcaaa gaaaatagtc ggccaggagc
    17101 gggatatcct cgggtctctc cctcggtgaa aactaatccg gatccaaacc taaagtcacc
    17161 caccactctc ggcaggatca acgctaccgt gcgcggccag cctggggcac ctcttggcga
    17221 gccattcggc gggccacgag ttctccagtt ggcagcgtgg tgggaattct gagaaccggc
    17281 gcttgcaatc attgaacccg gattctctgg ccagtgaaag tccctttcag cgagaaaata
    17341 agatttccgc ccggactcgc gagctgaccg ctgaactgcc ctgagattcg atccgtcatg
    17401 gaatgtagcg ttagatgcat gttcaccgtc atgatcccgg taagactcgc atccggcata
    17461 aaccagagtg tgtgaaacgt gagggcaaaa atattaaacc ctgtgcgttt ccaggccccg
    17521 tgtgccggtc cttccgttct caagccctgc aagaaagtac cgtcaccctg agccgtggca
    17581 atgaatgtgc cctccttatt gaacgtgcct agcgccaaag cctgtactgg tgacggcaac
    17641 acccgattca ggctaatcaa ccaggatccc gtcatatcat ttccctcgga ctgcgtgaac
    17701 tgttgcacgt tgagggaact cgtcatgatg gacacaacca tcagagtcag cacacgtgaa
    17761 aacgtcaact gcattgaaat tacctcccgc gccgagtttc cgatgaaggc gtcccgaaaa
    17821 ccatcagcaa ctcctgccac tgcggctgtg gccgggcttg gctgagaatg actcgaatga
    17881 tctaaaatgg tccgcgcggc cggagagatc ttcatgccca acggcggaac caataacgcc
    17941 cgcgacccga agcaaccggc gccgggggat cgactcgact cctggaagga gatcggcgcc
    18001 tatctcaagc gcagcgtccg cacggtgacc cggtgggagc aggagcaggg attgccggtg
    18061 taccgccata agacgggagc cgtttatgcg tacaagccgg agatcgatgc ctggtgggcg
    18121 gcccaccgga aacaaatcca ggtcgaaatt gacaacgagt ccgctgtcgc attgtcgccg
    18181 cggaaagccc gctctccatg gatagtggtt tcggccggag tagcagcctt gagcatggcg
    18241 gtggcgctcg gctggtttgc ctttcggtct ccgccgcgcc ggacggcgaa gtcggtcccg
    18301 ctgaccgctt acccggaaat tgaaggcccg ccaagcttgt caccggatgg caatcaagtg
    18361 gcgtttcact ggaagggcga tatttgggtc aaagctgtgg acggcgacgg cgtgcgaaac
    18421 atcactgaca cgccgggagc cgaagaggct tctcccgcct ggtctcccga cggccgctgg
    18481 atttcgtacg tcaaaaatcg cgccgaggtc atggtcagca ccccgcttgg tgggaccacg
    18541 acccgaatcg cttccatcaa ggcctcgcca tggggcggga ccctggcgtg ggtgcccgac
    18601 agcaagtctc tcgtgattgc tgaggttccc tcggaaggta acaagattca ggcgcatctg
    18661 gccatggtct cgattgccac tggagaaaag aagcagatca ctttcccgcc gggaccgggc
    18721 atcggccatt tctggccaac cgtttcgccg gacggccgga gcgtcgcatt cgccgagttt
    18781 ccgcaagatt cctccgccaa tatctacatt cagccgctcg acggcggcgt gccgcgaaga
    18841 atcaccgacg ataaggcaaa catcaggggc ctctcctgga cgcctgatgg gagggaactg
    18901 gtcttcgcgt cggatcgcaa cggagttttt cggctctggc gaatgacagc gcaaactgct
    18961 gcacagggta cccctgccgg actcgtcaga ttgctctcgg tattcctgaa catgcgttcg
    19021 cggcccgcgt cggcgcaaat cgtcgaaggc gcgggcgaag acgccagatt cccatctttc
    19081 tcgcgacccg gaccgggtag acccgtgcgc ctcgcgtatc aacaataccg ggagaaccag
    19141 tatatccggc ggactgcact cgctggcgat tcctcttcgc gcatgtcgtc aacgtctgag
    19201 cgtctcctct attcgagcag gagcgagtcg gagccgcagt tttctcccga cgggcataag
    19261 attgcattca cctcggaccg ctccggagca tgggatctgt gggtttccga tggccaacac
    19321 gaaccggtgc gccgcgcatc catggaaggt ccgccagtat tggggccacg gtggtcgccg
    19381 aatagtcgtc aactcgcctt ctttgccccc actggcgcag ctggcaatta ccagatttac
    19441 gtggtcgatg agtcggacgg aaaacctcag cttctcagtc gcgacgagcg gtacgccgac
    19501 tttttgccga gttggtccca tgatggccag acgatctatt ttggatcagg aagagggggt
    19561 aagacgcaaa tctggaagat gccggctcgg ggaggcaatc cagtccaggt caccaagggt
    19621 ggcggtggag acgcatggga gtctttggac ggcagactgc tctactatac gaaggttccg
    19681 gaggaagggc caggcttgtg gagcgtcccg gtcgacggcg gcgaagagca caaagtgctg
    19741 gacggtccat ggtttaaagg gtgggcgctt acgcaaaagg gtatcttctt tggcgattgg
    19801 gctgctgagg gcgatggtag caagccagtg aaattcttca ctttcaaaac gctgccgata
    19861 accattggtt ccgtggagaa gacggacacc ggttacctcc caagtttgac ggtcagccct
    19921 gatggccggt ggctgctgta tacgagccgg gaacgagtgg atgcagatct gatgctggtt
    19981 gacagctttc aataactgcg cgcctgccac ccgcagccat gaccggacaa aacagcgatg
    20041 acagccgttg gcagttgcta aaccgtggat gtcatttaaa tttgaaagca ggtaaaacac
    20101 ggcaactgcc tgatgcattg tgtgacctac atggatcctc ggtttgactg ctgttagcgg
    20161 acgcgcagtc cactacggtt ggcaatggct cacgtattcg aaagatcaac agagtggcac
    20221 aaccgccggc cacagatttg agtgaaccat gctgacccgc tacttactca tgacatcgac
    20281 actgttgctg gccgttgggg ccgcgtcggc gcagaacaac catgcattcc gaccggagat
    20341 ttcaagagtg gcagcgagtt gccctcgtaa gagaacgtgc gggtttccga agcgcccgtc
    20401 ttcggattgt gcacgcgcag ggtattgccg cgcacctcca ccgtgcggcc gtcccgcagg
    20461 cgattcgccg cgcgccgctg cgcctccgct tcggaaaccg gcgtggcgat ccagccggac
    20521 agacgaaagt aacgccccgc gatgctgccg tacaccttgc catctgtgcc gcggtagacg
    20581 acggcctggc cggtcacctg cgcgccggca ggcaggatgc cgcgatgctt ccccgtggcg
    20641 atctcatacg ccgtaatgtt ggccctgctg tagccgatgc cggcgtacat gaagccgtcg
    20701 tcgcttccgg ccacgtaatg cgcatagtgc tcggtgggat ccatgcggcc caggtcttcg
    20761 agcgcgccgg tggcgggatc gtatcgcaac agcttggcgc cgggatatgt gccgccgtag
    20821 agcttgccgt cggcgccgaa cgcaacgtcc cagatgaact tctcggtctt gctcgggcgg
    20881 ccgagatcga tcagctttcc ggctttgacg tcgagcttca ggaaatgcgc gcgcggaagc
    20941 gtgcccagat agatgttgcc gtccggtccc accgtcatgc agcgcgcgcc gtgctctccc
    21001 tcggccggat tgcggaacac ctgggcatcg ccggtcgccg gatccacggc caccacatcg
    21061 acggtattag ccagatacaa gtagctgaga tacagccgtt cgcctcccgc cgcaggtccg
    21121 ggccccacgg tggaggcata cattccgcca agcccgcgga atttcgccaa cagttcgaaa
    21181 cgtgagggcg cttgcccata cgtcagcact gccgcgaaca gccccaccag cacacggccc
    21241 ggaaccgcca accgtttcat cgcacacccg ccggcacgac tttctgaaaa gcactcacga
    21301 tatcgtcctc gtccgcggcc gtgagacacg acggaatggc tagcaggatg ctgcgctcga
    21361 acaggctgtc ggccaccgga cacgtgccct tcgcgtaccg cagcgccgag ccgctattgt
    21421 ccgccagagt ccaaggaaag ccccgtccgt cggtactcgc caggtccacc aggctggcgt
    21481 tattcgaata cagatgaagc ccccagttgg tcatgaggat attcgagatg ccccccggcg
    21541 aagttacgat gccctccgcg cgcagggcgg cattaacttc gcgcgcctct tccgcgccag
    21601 cgaagctgga gatgaggaag cagccggtgt ctcccgccga atccagcacg cggcgcagtt
    21661 tgacgccggg taatctctcc agttcccgcc gtatgcggta cttactgcgc cgcatggcgc
    21721 aaacgatgcg cggcagtttc cgcaattgca cacgaagcac ggccgcacgc aactcatcca
    21781 tgcggtaacc catgccccag agcgagaggt cggaggggtt caggatcagg cggccgttca
    21841 cgtcacgcgc atagcccaca tcgtgacagg ccaccgcgcg ccggtacagg ccggcatcac
    21901 ccgtcaccac gcagccgcct tcgccgcagg tcatgttctt gttcagttgg aagctgaaga
    21961 cgccgatgtc gccaaacgtg cccactttgc gcccgcccag cgcgccgccg gcgcactgcg
    22021 cgcagtcctc caccaaaaac aggccatgct tgcgggccag cgccgcgagc gcaggtgcgt
    22081 cacccggcgc gccgctcata tgcgccagta cgatgcccgc ggttcgcggc gtaatgcgcc
    22141 tctccacatc ggcgggatcc agacagaagg tatcatcgat atcggcgagg accggaatgg
    22201 cgccgcgact caccaccgcg cccacgatcg agacccatag atatgcaggc acgatgactt
    22261 cctgtccggg cccgactccc aaagcggcca acgcggtatg cagcgcgccg gtgccgctcg
    22321 acaccgccaa agcgtacggc acatcgagaa agcgcgcgaa ctcctcctcg aagaggccgg
    22381 tttcgttctg cggctcgggt ccgtaatacc ggaacagcga gcgcgagcga agaacgcgta
    22441 cagccgcctc cacctcctcc tcgtccatat aatgaacgcc cggatattcg agcggcaggg
    22501 gctgcgaccg caccggggtt cggccctcaa tcgcaatttt catggcttta tggtgagccc
    22561 ttcagtctct gctgtccatg ggcgaaattt gcgaacgatg gattaacgcg ccagacgccg
    22621 gcgttcagcg gtcacgtgcg ccataatctt cacgaacggc gcagtccaca ctcgcgcgcg
    22681 gttggcgtcc agccagcgca gcagcgcctg atgatcttcg cggtccacgt tgatgctatg
    22741 gccgccgccc acgccgtgaa aggtgaaaac ggccagcgca ccggactgca cggcctgctc
    22801 cacccacgcg atcatctccg cgcctttgtc gttggcgagg gcccagctcg gcacgcggta
    22861 cgggtcgagc tcgagcgggt ccgccagtgc gcggaacgca tgtttataag cgcgagcggc
    22921 gggtgcgagc ggccggatgg aatccacgaa ggagacgccg cggatggttt cgtcgccgca
    22981 ggtgtaggcc agcgtgtgcg ccttctcccc gtccaccgcg tgcaggaagg cgtccatcac
    23041 ccggaattcg gcggcgatgc gggcgatcga ataagtgttc agatcgcgct cggcgtccac
    23101 ccaagtgcgc tcgcggccat cggcctgctt caggcacgga tgaaacagtg tatggttgcc
    23161 cagctcatgc ccccgcgcgg cgatggcgcg ccactccgcc atgcgcttgc ggaagctctc
    23221 ggaatagccg gtcacgtaga atgtgccgcg cagacgggcg gcctccagat cggggacggc
    23281 gtgatcgagt tgcacgtcga ccccgtcgtc gtaagtgaga gccacggcgg ccttggcgcc
    23341 gtcaggccac cggaatccgg tctgctggcc gggcgccgcg ccagccatga gcagggcaat
    23401 caaaggaacg gcaatgcgca tcggtctcca gtataagcgc catttcccgg ccgaggcggc
    23461 ccatatgcgc aacggtgcca cggagatgcg ctcaagtgat tggcggcgcg caggatgagc
    23521 gtgcgcgggc ggcgcaacaa aatccaggtt tcgcaaagtc gctccattgc ggggctcccg
    23581 cgccattggt tagcatcgcg atcatgccgc gtttcttact ggctctagct gtcttcatac
    23641 tcgcctgggc tccgctcctg gacggcgcgg tgcgcatccg ccgcgatatc tcggataact
    23701 ggcagttcct gccggtggat gaagccggca tggagaacac cgactatctg agccgcgtgg
    23761 gctggctcac cgtaggactg ccgcacacct ggaacacgaa ggacattttc gacaacgtgc
    23821 cgggctaccg ccgcggcccg gcctggtacc gcaagtcgtt cgatatcccg gcggagtggc
    23881 gcggcaagcg catcgtgctg cgcttcgaag ccgccgcgca ggtggctacg gtgtgggtga
    23941 ataacacgct ggtgggcgag cacaagggcg cattcacccc gttcgaattc gacatcacgc
    24001 gcctggcgcg cgtgggcgga gacaacctgg tggcggtgcg tgtggacaac cgctggcggc
    24061 gcgacgtccc gccctacgac atggacttta acatgatggg cggcctgcat cgtgaggtgt
    24121 acttgatcgc caccgcgtcg gcctacatcg cctctacgcg cgtgaccacg cctcgcgttt
    24181 cggaaggtga agccgttgcc gcctttgaag ccgaggtgcg gaacgaagcc tccacccagc
    24241 gcgaggtcga gattgttacg caggtgctcg attcctcagg cgcgccggta gagacgctca
    24301 cctccaaagc agcgatactg gcgggcgcgg cgcacacctt ccggcaggag acgcggccca
    24361 tcctcaagcc gcggctgtgg tctccggaca cgcccaacct ctatcgtgtg cggtttgaaa
    24421 tccgcgagca aggagctgcc atcgacgacg acgagagccc gctcggcttc cgctggttcc
    24481 gcttcgatcc tgacaaggga tttttcctca acggtgcacc cctgaaacta cgcggcgcca
    24541 accgtcacga cgattatccc ggcctgggct gggcgctgcc gccttcgcgc catgtggccg
    24601 atctgaaact actgaaagag atcggggcga acttcctccg tccggcgcat tacacgcagc
    24661 accccgtggt gctcgaaacc tgcgatcgtc tgggactgtt ggtgtgggaa gaggtcccgt
    24721 tcgacggtga gggcgaacaa ctggcgccct atgccggcgc gtccggcttc gctgaaacgg
    24781 tgaagcagat tctgcgtgag accatccggc gcgaccgcaa tcacccctct gtcatcgtgt
    24841 ggagcgtggg gaacgagaac ctgaacggcg ccaccgagaa cgagtggcgc gccgtggccg
    24901 gccttacgaa agagcttgtc gcgctcgcta aagaggagga tcctacacgg cccacgggcg
    24961 tcgccatcaa ccgcttcgac cgcgccgagc acgtggggct gatggaactg gtggacgtgg
    25021 tgggcctgaa cgtgtactac gggtggtatg ggcagacctt cgaggacttc cctccgctgc
    25081 tcgacgccct gcatcgcaag caccccacca agcccctgat cgtcaccgaa tacggcgcgg
    25141 acatggggcg cggccttcat agcgatacgc cgcgccgcta cgatttctcc gaggaatggg
    25201 gctgtctcta tcacgaggca tacctgcgcg agatcgacgc gcggccgttc gtcgccggca
    25261 gcctgatctg gaacatcttc gatttcgccg ttgaaaagcg catggcgcag accatccccc
    25321 acatgaacca gaaggggatc tacgactact accggcggcc caaggacgtc tattatttct
    25381 accgcagccg ctggacggaa gagccaatga tatacgttgt ctcgcacacc tggaccgaac
    25441 gccagggcgc gccgggcgag cccaagcagt tcaaggtcta cagcaattgc gaccgggtcg
    25501 agctgttcgt caacggcaag agcgcgggcg tgcagcccgg aggcgcgccg gtctggaaag
    25561 tcctgctcgc gagcggcgac aacgaaatcc gcgccgtggg ccgcaaaggc gaacgtgagg
    25621 taacagaccg cctccgcgtg cgctactgag cgatcgtagc cggcgccttg cttccaggtt
    25681 gggggctgcg tggggttttt ctgaacccag cacttcatta ttgcgcaacg aacgcgcaat
    25741 tttgctttac acgcctagaa ggatgcagta tccttgccct aggagggtgc ttgcgtctga
    25801 ggccatttcc gctgtctgcc accgcagttt catgttaggc cgcatccgtg ggctccgatg
    25861 gtgggtgatt ttcacctgct ttctggcgac cacgatcaac taccttgatc gccaatgcct
    25921 ctcggtgacg gcagcggtac tttccaagga gttcgggctc tccaatgccg actactcctc
    25981 gatcatcacc ttcttcctgt ttgcgtacac gctgatgcag gcggtctcgg gccggatcat
    26041 cgaccgcatc ggagtgcgca ccggaatggc gctgtcgatt ctgatctggt cgtttgccgg
    26101 aatactgcac gccttcgctc aaggtctttg gtcattgcgg atattccggt tcctgcttgg
    26161 cttcggggag gcgggaaatt ggccggcggc caccaaggta gtgtcggaat ggttcccggg
    26221 ccgggagcgc gccttggccg tagcgatctt cgatagcggt tcggcggtgg gcggcatcgt
    26281 ggccgcgccg ctcattgcgg cgctgacctt gcactacgga tggcgtccgg cgtttctcat
    26341 tacggggagc ctgggcctgg tgtggctctg gttgtggctg tggatttacc gcaagcccga
    26401 ggagcacccg agaattacca aggaagagtt gcagtggatc cgccagggca acgcaaacca
    26461 gttggagccg caaggcgccg tggtcacctg gaccggactt ctcagggtgc gggcggtgtg
    26521 gggtgtaatt ctagggcgct tcctcacgga ctgcgtatgg tggttttatg tctattggct
    26581 gccgaaatat ctggctgatg tccgcgggtt taccctatcg cagatcggcg ccatggcgtg
    26641 gatcccgttc gtggcggtag atctggggaa tgtgggcagc ggctggattt ccggccggct
    26701 catgcggaga ggattcagcc tgaacgccgc ccgcaaggcg gtcctgttgt tggcggccgc
    26761 cggcatggta gcggctcttc cggccggcac gacggccagc gccgcattgt gcctggggct
    26821 gatcgctgtg gcttgctttt cgtatgcggc atggggaacc atgatgctca ctctgccgtg
    26881 cgacttgttt gatgcacgca tggtggcgag cgtctctggt ctttccggca tgggggcggg
    26941 gctgggagga atcctgttca cctgggcgat cggagtcgtg gtcgaccggt tttcctacca
    27001 gcccatcttt gtcgctgccg gcctgctgcc gctgctcgcg atgctgaccg tgcagtgcct
    27061 gattcccgaa gtgcgccgca tggcgggcgc gggagcggcg gcacatcgaa gtggagaagg
    27121 agtttcgtga acgaagaact gaagtatggg gtcctgtaat atgacacggt gtttccagat
    27181 ccatcccgcc gataacgtgg caacgctgtt agcggatatc gtggaaccgg gcgttgccgg
    27241 catcctggga ggaagcatcg gctctctcga ggtgcgggaa aacgtgccct tcggccacaa
    27301 ggtcgccgtc agccgtatcg ccgccggcga tgccatcgtc aaatacggga cgacgatcgg
    27361 cgtcgccaca cgagagatcg agccgggcga gtgggtccat ctccacaact gccgtagccg
    27421 ggtggatgag aagtcgtcgc aactcgatgt gcaaaccgga gcccgcacgg agaccgtcta
    27481 tgagtgagcc tgtttggcag ggacatctcc ggccggatgg acgcaaggga atccgcaacc
    27541 tggtgctggt tgtttacacc gtggaatgcg ccagccacgt ggcacacgcc atcgcatccg
    27601 gcgaagaggc gacgcatgtg atcggctttc ccggttgtta cgacaacgac tacgcgatac
    27661 gcctgatcct ggcgctggcc cgccatccca acgtgggcgg cgtgctggcg gtggggctgg
    27721 gatgtgaata cacgcgcccg gagcgtatcg ccggcgtagc ccgcgcctcc ggccggccag
    27781 ccgagtggtt caccatccag gaggcgggcg gcacacgcgc gagcattgcc aagggcaaag
    27841 ctcttgtggc cggtctccgg gatcagatga gccgctccga tgttcccgtc cccatgacct
    27901 tcgcagacct caccgtgggc gccgaatgcg gcggctcgga tgcgacctcg gggcttgctg
    27961 cgaaccccgt agtgggggct tttttcgatc tgctggtgga ccgtggcggg agggctatct
    28021 tcgaggagac cgtggagatg atcgggctgc gcgacacctt gcgcagccgc gcagcgtcgc
    28081 cggaggccga gcggcagctt gtggcgagct atgacaaggc ggtgaactat tgccggtcgg
    28141 ttcgccagta ttcgatatcg cccggcaact tcgccggcgg tttgaccacc attgaagaga
    28201 agagtatggg cgccttctcg aagagcggaa gccgtcccat tcagggtgcg atccgtgtgg
    28261 ccgaggcgcc gcccgggccc ggactgtggc tgctcgacag cgttccggac gagtatttca
    28321 tgggctttgg ctacaccaat ccgaacgaca ccgaaggcgt gatggatttg ctgtcggccg
    28381 gtgcccagat ggttctgttc atcacgggac ggggaagcgt gatcggcagc cccatcgctc
    28441 cactcgtcaa gatcaccggc aactcgcaga ccttccggcg catgagggat gacatggact
    28501 tcaacgcggg ccggatcctg gagggggaac tgtcgatgga agcggccgcg cgggaactcg
    28561 gcggcctggt cgcgcgcgtc gccgcgggcg aagccacctg ctcggaggcg ctcaatcatc
    28621 gcgagtactt catcatgtac aagcatcagg acacgcctgc tctggccgag gggtgccgtg
    28681 cgtaagccgt tccggaacac gcaggaagcg tttggcctgg acggggaaac cgcgttgatc
    28741 accggcggcg gtaccgggtt gggttttgcg atggcgcagg cgttcgtggc gtccgggtgc
    28801 cacgtggttc tggcggggcg ccgcgaaacc ttgctggccg aggccgttgc ccgcctgggg
    28861 ccgcaggcct cgcaccgcgt tcacgatgtg agccggcatg accaggccgg gccgtttgcc
    28921 gactcgctgg agaagcaatt cggattcatc agcatcgtgg tgaacaacgc cggcatccac
    28981 ctcaagaaag cggctgcgga gacgaccgaa gcggagttca gcgcggtgct ggacacccac
    29041 ctgacgggag cgttcgccat cacgcgccgg ctgatcccgg cgatggccga acgccgtcat
    29101 ggcagtattc tgttcatcgc gtccatgaca gccttgttcg ggatgcccca cgtggccgcg
    29161 tactcggcgg cgaaatccgc ctgcgttggt ttgacacggt ccctggcctg cgaactcgcg
    29221 ccccgcggga tacgcgtcaa cgccatcgcc cccggatgga tcgatacgcc catgctccgc
    29281 gaagcgctgg cgcgcgatcc ggagcgggaa cggcggattc tgtcccgcac cccgcagcag
    29341 accttcggca gcccggagga tgtcgcgtgg gcggccgtct atctctgttc ccccgccgcg
    29401 gggttcctcg acggcgtagt gctgccggtg gacggtggcg ccagcatagg cttctgatcg
    29461 tccgctcagg gttgcgatgc cagaacctgg cgcgccggcg gaccggcggt gccggccagc
    29521 ggtggagcgc aggattcgcg gaggatcacc tgggtgggaa tggttctcct ctgcgggggt
    29581 agatccggct ggctgacgcg attgagcaac aactccgcga gcgccttgcc aatctgttcg
    29641 gagaagaccc gcacggaggt caggggaggc cgcatgttct ccgcttcaat gtcgttgtat
    29701 ccagccacgc tcacatcggc ggggacacgc aggccggctt ccgccagcgc ctggtatact
    29761 cctcgcgcga cgacgtcgcc tccggcgaac aaagcggtca ccgggagccc ccggttcaga
    29821 atactcttgg tcgtgagata gccgagttcg tgttcgtcct cggagtcgaa ctcgctcaat
    29881 cggggctcgc agcccgcgtc cgtcatggcg cggcagtagc cctcgtagat atgggcatgc
    29941 cacgtgagac gtgtgttgcc gacgtaccag atgtcgcgat ggcccagagt gagcaggcac
    30001 cgcgtcacat ccatgccgcc ctggacgtcg tcggaccata ccgagtcgat cttgtcctgc
    30061 ttccacggac ccgctacatt gttgccgaac accacgaagg gtaccttgcg gtgcgccagc
    30121 agatcaaaca ggttctgcgt attggtgccg gcgacgatga atccgctcac gaggtcgcgc
    30181 cgctgcagca gcttgggcag gtgcacctct cgccacgggg tggtctggga gtagcgcaag
    30241 gagagataca gcaggttgta atctctgcgc gcgaggtaga cctcggcgct tgccagaacc
    30301 agcgagtgga agtagtgcag gacgtcgcgg ttgctgaaca ggaaggtgat gatgcgcgtg
    30361 ccgttctccc ggttcaggct aaagccgagc tcttccgccg ctcgccggat gcgctcctcg
    30421 atctcgggag tgacccgcat ggtgccgcgg aggagccgag aaaccgttga tgcgctgaca
    30481 tttgcgcgac gagccacagc attcagggta ggcttggtaa ccttcatgtt cgcttctgat
    30541 tcccgagttc agtatagcct gtgctgaagc aaaaggtgcg aaattctgtg cgcgagttgc
    30601 gcttaaagag gcgcatgaat tgtgcaaatt tgccttgaca ttgcgcaaca tacgaaataa
    30661 gatttggttt acctgactgg gaacactcag ggggaggcta gtggcgcccg cgcacgggtt
    30721 ggatcggtaa cattgtccgc tcggcgggaa accatagccc aattcaggca aaaggagtct
    30781 cgctatgagg cgatccgttc gctcgctgtt gggatcactc tgtctattcg cggcggtctt
    30841 taccagcccg caatccgcca ttgcgcagga cgcgcgaggc atgatcacag gcgtcgtcac
    30901 ggacgcctcc ggcgcactcg tgccggaggc agtcctgcgg gctgtcaaca ccgccagcaa
    30961 cgtggcggtc actgcggcca ccaatgcgca gggcaattac accctgaacc acctgcttcc
    31021 gggcgaatac cgcatctatg tgtcgaagga gggattcaaa accttcgagc ggggcgtgga
    31081 ggtgcgcgcc gcggaccgga tgacgctgga tgtgcagttg gagatcggcg cggccagcga
    31141 gaggatcgtg gtggaggccg gcactccgtt gttggaagtg gcgagcagtt ctctcggcac
    31201 cgtcatcgac caccggcgca ttcaggatct gccgctggtg cacggcaatc cgttcatgct
    31261 cgagttcctg agtccgggaa tcaccttcaa cggcaacatc gcctggacga ggccttttga
    31321 tggtccggcg tcggtggctt ccgtgaatgg ctcccagacc gcccgcatcg agttccaact
    31381 cgatggcgtc gccaacaact ggcggcggag cccggcctac cagccgtcgg tggagtttat
    31441 ccaggaatac aaggtgcaga ccaccagcta tgacgcggcg caaggtcatt ccgcgggggg
    31501 ctgggtggat gtggcgctga aatcgggagc caacgatctg cacgggtccg cttactacta
    31561 caggcaggat ccggcgctga acaccaacct gttcttcaat aacaaggccg gccagccgaa
    31621 gccggactac atgttctacc gcaccggcgc cacggccggc gggccgatcc ggagggaccg
    31681 cacattctgg tttgtgggct atgagaggat tcgtcacaac ctgccatacc cgcaggtcta
    31741 caccgtgccc accgaagcgc agcggaacgg cgatttctcc gcgctgctcg cgctgggcgg
    31801 ccagtatcag ctctacgacc cggccaccac tcgcccggcc gaagccggac ggtactcgcg
    31861 gcagcctttt cccggcaaca tcattccagc cagccggcag agcggcatcg gaaagaatat
    31921 cgtcaactac taccccctgc ctaaccagcc aggttcgcgc gacggcggca gcaatttcaa
    31981 cttcggcagc gggattgagc cggatcacta ctacagcttc accacccgta tcgaccacac
    32041 ctttagcgac cggcagcgga ttttcgggcg cgccgtggtc agcaagcgtg tcgacgggcc
    32101 ctaccgcgat tgggcgcccg atgcttcggg aaacaacctg tactacaaga accgcggcgc
    32161 cgcgctggac tatatcaaca acctgacacc gcagacggtg ctgaacctgc ggtacggcta
    32221 tacgcgcttt gtctcccagc acgtgcttga gacgctcggt ttcgacatca cctcgctcgg
    32281 gctgccggat tcgctccgcg acagcatcga ttcccgcggc tggatcttcc cgcggatctc
    32341 gccggccggc tactcgggcc tgatgaccga acaactcgac gggaacttct ccgacatcca
    32401 taccttctac ggctccgtct cgcgcacgat gggcagccac ttcctgcggg taggcgccga
    32461 tttccgttcc tatctggtca acaactatgc tctcggcgcc agcaatggaa actacacctt
    32521 cggctcctac ctgagcggtc cgctggacaa ctctccggcg cccccgcgag gcaccggcat
    32581 cgccgggttg ctgctgggcg tccccgatgg cggcggcgtc gatgtgaacg actcttacgc
    32641 ggcacgatcc catttcggca gcgtgttcct tcaggacgac tggaaagtga cgcgcaagct
    32701 tacggtcaat ctgggattgc gctacgagta cgacggcccc atcgcggaac gattcaaccg
    32761 cagcgtgcgc agcttcgact tcgccgcggc gagccccatc gagccggcgg tggtggcgaa
    32821 ttatgcgagg tcgccgatcg atgagattcc cgcctcccag ttccgcgtga agggaggcct
    32881 gactttcgcc ggcgtgaacg gccagcccgc cgagctttgg caggcacctc ggagaaacct
    32941 tatgccgcgc attggattcg cctatatgct cggcagccaa acggtgctcc gcgcagggta
    33001 cggcgtcttc ttcgatcaga tcggcatcgt ttcgcagagt ccgatccaga tcggctacag
    33061 cctcaccacg gcgctggtgc cctctctcga taacggcgtg accttccgcg ccaccctggc
    33121 caatccgttt ccggacggac tgcaaaagcc ggtcggagcg cgcgacgggt tggcgacgtt
    33181 cctcggccgg ggcgtcagtt tcttcgtgcc cgacccccgc actccctaca atcagcgttg
    33241 gtcgttcgga tttcagcggc agttcctgtc gaacttcgtg ctggacatga actatgtcgg
    33301 cagccgcgga accgcattgc agatctcccg gcaactggac agcgtgccga acgagtatct
    33361 gagcaagttg cgcgttcggg atcaggcgac catcgaccgc ctttcccaga acgtcgccaa
    33421 tccgttttac ccgcttctgc cggggaccgg cctcgccggg gacacggttt cccggtcgca
    33481 actgctgagt ccgtacccgc aattcaccgg tgtcaccacg cagaccaacg acggatactc
    33541 ctggtatcac tccctgcaaa cccgggtgga gaaacgcttc gatcgagggt acacgatcat
    33601 gggttcgtgg acctggtcca agaacatgga agccgtcagt ttcctgaatc ccatggacgc
    33661 acggccggag cgcgtgatct ccccgaacga tcgcacgcac cgcctggtga tcaatgggat
    33721 ctatgaactg cccttcggta tgggccgccg ttatgcggcg ggcgcgcggg gtgtggtggg
    33781 caagttgatc gaaggatggc agtttgacgc catctaccag atccagagcg gagacccgct
    33841 cggactggga gactttctgt tctatggtga tccggacaat atcgtgctgt ccggagggga
    33901 gcgccgcccc gagcgctggt tcaacacgga cggcttcgaa cgggacacgc ggcgggcgct
    33961 cgcttcgaac attcgctacc agtcctcccg gttctcctca ctgcgctcgg acggactcaa
    34021 ctacctggat ttctcgttga tcaagaaaac gaagatcacg gagcgcattc gtctggacat
    34081 ccgctccgag ttcatcaatg cgctgaacca tactgtcttc gaagcgcccg acacaaaccc
    34141 atatagcgcc aacttcggag tcgtgacggc gggcaagcag cttccgcgca cgatccagtt
    34201 cggcaccgtg gtccgcttct gaccggcgcg ctcgcggacc tggctatcgc ccgcttggct
    34261 aatatcgggt gagtgaagag atatcgaggc aggcctccac agatgatctc acgttctctc
    34321 gtattgctgc tggcttccgc tttggaactg gcagcccaga ttccggcgcg ctggctcact
    34381 gagccggtgc tgccggaagc cacccggcgc gggcagatgt acgcctacgt caacgccaac
    34441 attcctccgc tgccgcagtt tcgttcgctg cccgagtggg agcgctatcg cgtcacggta
    34501 aagcgcgacc tcctgcgcct gttggggatc gacgatattc tgcggacaca ccgcctgcag
    34561 gtggtaaaga aggggacgct gcagcgcaag ggctaccgca tcgacaagat cgtatacgaa
    34621 agctacccgg gcatgtatgt gcctgctctg gtatggagac cggatcgggt gggcgccaag
    34681 gcgccggcca tcgtcagcat caccgggcat acctactgcg acagcaaggc cgcggactac
    34741 atccaggcgc gcaactacaa tctggtgcgg cgaggcttca tcgtcgtctc ctacgactat
    34801 ttcggctgtg gagagcgggg gcggctcgac gcatgccaac caggggtctg gggcggcgtc
    34861 gatcatacga actcgctgtt ctcctacacc acccgcacgc cgaccggaat cgaagttctg
    34921 gacgggatac gcgctgtcga ttatctgtac tcgcttccgg aagtcgaccg ctcgcgcatc
    34981 gccttcaccg gagaatccgg gggcggcaac aacacctact gggtggcggc gctcgatgaa
    35041 cgggtgaccg ccgccgtcac cgtctgcgcc gccggatcga tggcgcagtg gatcaaggcc
    35101 gatctcaact acgattggca ccagcgcccg ccgggaatgc gcgcgttcgc cgacatcggc
    35161 acactctacg ccttgacggc gccgaggccg ctgctggttc tgaacgggca caaggacctg
    35221 gctgagtttg catggcccga cgcggagcgg tccgtggact atgcccgcgc cgtctatcgc
    35281 ctgtatgggg cggcggatcg catcgcattg gagcgctcca cgaccgatca cggctaccaa
    35341 ctggacaagc gtctgcggct atatgactgg ctgggccgct ggtatttcgc cggtaagact
    35401 ccaggcggag ccgcggagat cgatgcgccg gtcgaaccgc gagaggcgct gcgcgtaggg
    35461 ctcagcgaag ggctgtcgat cccggaactg gcaaagcggt ggatggacga gacgcgccag
    35521 gaggtcccgc tcccggcgaa tgcaaacgat gcccgcacct ttcaggagga tcgccgccaa
    35581 tcgctggcaa agctcctcgg cctgcgccag tggggggaaa agcccggcgt ggcggagcgc
    35641 caaacggacg tcgttgcaga aggtgaccag gaagccgagc ggctcatctt cgagatggag
    35701 cccgatctgc ttgtgcccgc ggtgttcgtg cggcctgtag ggcggaagcg ctatccggta
    35761 gtcatcgtcc tggagaagaa acgtgggtcc tcggccgaag tgcgggaact gctgcgcggg
    35821 ggcgctgccg tgctgctgct ggatccacgc ggcgccggtg aaatggactg gggcggagga
    35881 cgcacttcga actgggccaa tctgaccggg cggaccgcag ccgggatgtg ggcagaggat
    35941 gtgtcgcggg tcaccaccta cctgcttgcg cgtccggacg tggagcgcgt cgccgttctg
    36001 ggctacggca tctttggcaa ggccgctctg tatgccgcgg cgctcgacag ccgcatttcc
    36061 gcggccgcgc tggcgatgga cacaatctcc tacagggcag aggccgaggc gggatttgcg
    36121 catgtctatg ccgacgtgcc gcgtatcctc gcctggggag acacgccgca gatcgccgcg
    36181 ctggtcgcgc cccgggcttt agcgattctg agggcgggag agccggtctc gtccaatcag
    36241 gaaaggctgt cctacttttc gccgatgccc cgcttcgatg ccggaccgcc gtgggtcgcc
    36301 gaggcggaac tccgtgacgc gtattcgtgg acgcagaagt tctatgccgt tttcggggcg
    36361 gcaagtaggc tccggctcgg cgcaagtggg gagttggaga cggcgcaatg gctagtggct
    36421 ggcccatgac gacaaaacga ttgcagggat ccaaagaggg catgacgcgc agagccgttc
    36481 tggggctgct ggcagcgccg gcagcggtcg aggcgagtca ggcagtgggc acgaagaaga
    36541 aggtgattgt cgccggcgcc ggactcgcgg ggctctgttg cgcctacgaa ttgcgaaagc
    36601 gggggcacga cgcggtcgtt ctggaggcgg ccggccgcgc cggaggacat gtgctgacgc
    36661 tccgcgaccg gttcgccgac ggactgtacg cggacgccgg agcagaacat ttctatcgtc
    36721 ccggctacga tcagctttgg agctatatcg acgagttccg gctgcctgtc attgcctatc
    36781 cccgccgcca ccgcgtgttg cgtctcgtcg agggccgcct gctcgggccg gaggagttgg
    36841 agagcccggc gcgattgcgg gccgccggct acaacgagcg cgaactgcgc tacatcggcg
    36901 agcactcctt acccggcctc ccgggcctgt atttcgatcc ctacatcggg cgttttgaag
    36961 acgagtacaa gccgtttgcg gcgggcctga accacctgga cgccatgacc ggacgcgagt
    37021 tcctgctgca gcagggagcg tctcccgcgg cggccggttc gctcggcgga cgccgctccg
    37081 ctctgcaggt ggtgtggcat gcagccatcc gccggaagcg gggcatgaag tggttggaga
    37141 cgaatttgtt tcggatcgaa gggggcaacc agcgtctcac ggacgctttt gcagtccgcc
    37201 tgagcggttg cctgcggctc gggtgtccca tcaccagcat cgagcattcg cctcagggcg
    37261 tgcgcgttca ctacagcgat aacggacgga tcaagcagga ggaagccgac caccttgtga
    37321 gttgcatgcc actcatcaac ctgcgcaaga ttcccctgac gccgggttgg ccggagacca
    37381 agcaatacgt gattcagaac ttgccatacg actcccactg ccgcgtcatc tttcaatcgc
    37441 gcacgcgctt ctggaagagc gaccgtgtca gtcccaacat gctgcttccc gggcccagcc
    37501 tgggttccgt ctggtctatg gcggaggagg tcccaaccgc gcgaggcatc cttatcggca
    37561 cggcggggat gacgacagcg gataaggcgg tggcggctta ccggcgcgcg tacccaggcc
    37621 cgtccgaaga tatcgaggag agcgtcgtgg tcaactggcc gaacgatccc tgggccatgg
    37681 catgcctgcc gacgggattg ccgcccggag aactgacgaa gtattggccg gcgatgatcg
    37741 agccgttggg ccggatccac tttgccggcg tgtacgcgga caactatccg ttcggaatgg
    37801 aggccgcagt ccggtccgcg cggcgggccg ccggcgagat caccagtgtt taagtcgaat
    37861 cgacagatcg tcatgcgaaa agtatggatt gcactgctat tggggcttag caccgccccg
    37921 gtctctctgt gggcgcaagg agccgtggag actcaacgcg cccgcatccg gcgctatccg
    37981 gccgtttggc ccgcggggcc ggctcccggc gccatcccgt cgtgggcggg gcctgggaag
    38041 atacgcttct cccgttggga tggtggccgc atcgaaacgg caaaggcctt cctttcgggc
    38101 tggccgggct tgaatccgcc aaatccggac tacctctata cgatgacgaa ctggtacgac
    38161 ccaaagaccg tgcagttcgc caagcaggcg ggcctcaatc ttgtctgggt gaccttgagc
    38221 gtcggcttct ccgatgagac cgaggccgcg catcgcgagg aggttcggcg ctacatcgac
    38281 gcgtgccacc gcgaggggat acgcgtgatg gcctatgagt cgatcggcaa catgttctgg
    38341 gaggacatgt ttgagaaggt ccccgagtcg aaaacctggg tccagctcga ctcgaatggc
    38401 aagccggtac catacagcgc cgccacattc aagatgatgg gccgcattac gcgctacatg
    38461 gcgaacctgt cgcatccggc atggcgcgcg ctcctgatga agcggatcga cctggcgatt
    38521 gagtccggcg ccgacgggat catgtacgac aacaacttcg gcgcgacgct ggttgaactc
    38581 tatcagcaga tcatgcgcca tgccctgagc cggaagaaag actttctggt catggcgaac
    38641 ttccacaagg acacctacgt tctgaaccgg cttttgaact gcatcaccac ggaagacagc
    38701 atcgagcccg ggctttactc caccgcttcc gaagggtacg gagacctgaa gccgcactat
    38761 ccctaccacc tcgcggtcgg ggacaagaag ctgatcaaca acttcgggtt gatgcgcatt
    38821 catgaaacgc tgtcggaagg atggaagccg gtcatgatcg aagccagccg ccgtgaacac
    38881 caggagcgca tggtcggctt catgtcgccc caacggtcgc aattggccat ggcggaagca
    38941 atgatgtttg gagtgggcta tgaacactac gtggagggca ggccggcgca tctactaagc
    39001 attggcgacc cggaggcggt ggctacgtgg caggccatcg gcaagtacaa ccgcttcttc
    39061 gccggccacg aagacctata tgtgggggcg cgatcgaaag cgccgctggc cgtcattctg
    39121 gatgacgaga gcgccggagt cgagcttttc gacggccttg cagctcgggg tgtgccgttc
    39181 gaggtgctgt acgagaagga cgtggaccgc gccgcgctct cgcgcttcaa ggccatcgca
    39241 ctcctgacag cccgctcggt gcgggcgccg gcgctgccgg ccatggaaga gttcgtgcaa
    39301 aagggcggcc gtatcgtggc cgcgggcgat gcggccacgc tggatgagaa cggcgcgccg
    39361 cgggcgcgtc cctcgttcct cgcttcgggc aacaaggtaa tcatctatga tcgcctcccg
    39421 ggagttgacc agcttgcggc aactctacgc gaggtctccg gcgagccgcc ggtcactctg
    39481 gacgggccgc ccgcggttct atggaatctc acggtgcagc cccaccagaa acggacgctc
    39541 gtgcatatcc tgaactacac gctgcagcca atccggaatg tgaggctgaa ggtgcaggga
    39601 gcctattccg gcgcgcggct gatttcgccg gatggagatc agacattgtc agcgcccacg
    39661 ctgggcccat cggcggccag ctcggcgatt ccggaactga agatctactc ggtggtcgcg
    39721 ctcgattgga agtaatcggt tgtcactccg aaagggaacc catgcgtatc ggcattacct
    39781 atctttatac gattttcaag tacggctatc cgcatactgt ggatgacgcg atccagtcct
    39841 taagcgagat ccaggagctt ggcttccact acgtggaatt ggagggattg gggcgtgaga
    39901 acctgatcgc ggtgcatgcc cggcgcaagg agttgcgctc ggtgctggac gattgcggct
    39961 tgcacgtgca caacttctgc gtcgtggatc cggatatggt ccacctggat gtggctgtgc
    40021 gacagccggc gctcgatcga ttcaggctgg gcgccgagat cgcggaggtg ctggaggcgg
    40081 agacgctgca tctggccagc tatgcgcctc cggtcgagta cctgcaagcg gctccctatc
    40141 agttgtccga aggcggttcc taccgatttg aagacgtcac gcgcttgcgc ctgccgcaag
    40201 gcttctgttg ggatgcggtg tggaacgcac tggtggagtc ggcggggttc tgcgccgatg
    40261 tggcggccga gtacggccgg acggttctta tggaaccgcg cgtgggcgag gtgatctgtt
    40321 ccgtggactc gttgcggagg ctgatcgagc acgcgggccg tcccaacctg aaagcgaatt
    40381 tcgacaccgg acatttctcg gcacagcgcg agaacgtcgt gctggcactc gcgaagctgg
    40441 aaggccattt cgcgaacgtt cacgtcgcgg acaaccatcc ggccaacgcg gagcacattc
    40501 ccataggtga cggatcgatc gattggcggg aattcctgtc cacattgaag cgcaccggct
    40561 atgacggcta tctcggactt gacctcggac tgagcgatca actcctcgat ggctaccggc
    40621 gctcagtgga gcatctgcgg tcgatcggag cggagctgaa catagagatc gaagtctagc
    40681 ctcgatcccg tctatcggac gaaacattcc tcgactctgc gcgattcgtg gactcgaatc
    40741 ccgcccaatc ccaagcttcg ggacaaatat actggatggt agactcaaat taaggggaga
    40801 ttccgcgctg aaaatcctct ttatgccagc caatcggcac gcctcgtgtg ggcatgcgct
    40861 ggatcaaatt atgataatca ggttaatcgc ttcgatcatt ctgctctgtg ggagcgccgc
    40921 gctggcacag cctgcggaag ctccggtatc caccgatcag gctctcgcga aactgctcca
    40981 gggcaaccag cgctatgtgc gccataagga acagcacccg cataaatccc tgacccggcg
    41041 caagagcctg gtaggcgggc agcatccatt cgccgtgatt ctgggctgct cggattcgcg
    41101 cgttccgccg gaactgttgt tcgaccaggg actcggcgat ctttttgtga tccgcgtggc
    41161 cggtaacatt gtggacgacg tcgtcctggg cagtattgaa tacgctgtgg agcacttggg
    41221 caccaagttg gtcgtggtcc tggggcatga gaagtgcggg gcggtatcgg cggcggtcgc
    41281 gggcggacac gacgagggac atttgaaagc gctggtgaca gcgattgagc catcggtttc
    41341 agccacggtg aatctgccgg gagatcgggt tcatttgtgt gtgattgaaa acgcgcggca
    41401 cgtcgcgcgg cagatccggg aatcccaacc gttgctgcga gaggcggtcc gtcggaatgc
    41461 gctgaaagtc gtagcggcgg actacgtcct ggagaccggc aaagtcatcc tcctcgagga
    41521 acaccattag gcacccgctg cgggcggctc cggcgcggac gaacgtcgtg ctgtttctgc
    41581 aaggcgtcca ctgcgagatc cgggccgttt tttccgttac cgtgcacgac agagttttcg
    41641 ccgggtctgt ccggttcgac catcggcctc gccacccggc ccgacgtggt ccccggccat
    41701 gacccgaccg ggccgcagac ggtacagcgg tggttcaaca ccgcggcctt tgccgcgccg
    41761 gcctatggct actacggcaa tgccggacgg aacatcctgc gcggaccggg catggtgaag
    41821 tgggacgtgt cgctgctgaa gagcttccgc gtacgcgaga gcctcgcatt ccagctccgc
    41881 ggcgaggcgt acaacgtgct caaccggacc aacctgtcgg gcgtcagcgc ggctctcggc
    41941 agcgcgaact acgggcaggt taccagcgcg cgggatcccc gaagcatcca aattggtcta
    42001 aaaatggagt tctaggtcca ttccgatgaa gatgaagaag aattactccg gacgggtcgg
    42061 gctggctttg ctcgttctcg tttcccccgc ggcgttcgcc ggcgaatatc acgtttccgt
    42121 gaacgggcac gactcgaacg acggctcctc ttcgaagccg ttccgtacga tctcggcggc
    42181 agcccgcgtg gcgcagcccg gcgacgtcat tacggtgcgt gccggtacgt accgggagcg
    42241 cgtcacgccg ccgcgcggcg gcacctccgg cagcaggcgg atcgtctacc aggcggcgcc
    42301 cggcgagaag gccgtcatca agggttcgga ggtgatccgc aactggaagc ctttcctgcc
    42361 cggcgtgtgg aaggcggtgg tccccaactc gctgttcggc aagtacaacc cgtacaagga
    42421 tctgatcgtg ggcgactggt tcaacaacaa gggccgcccg caccacaccg gcgaggtcta
    42481 tctcaacggg aagtccctct tcgagaccca cctcctcgag cgcgtgctcc atccggtggc
    42541 gttcgctgac acaagggacc gcgagggatc cacctacacc tggtttgccg aaaccgacga
    42601 ccagtacacc tacatctacg ccaacttcca ctcggccgac cccaacaagg aactggtgga
    42661 gatcaacgtg cgcgacagtt gcttctaccc caaccagccg gggcgcaact acatcacggt
    42721 ccgcggcttc cacatggcgc acgcggccac gcagtgggcc gcccccacgg ccgagcaaat
    42781 cgggctgatc ggcacgcact ggagcaaggg ctggatcatc gaaaacaacg tcatcagcga
    42841 ctccaagtgc tccggcatca cgctgggcaa ggaccgcgcc accggccaca acgtctggat
    42901 caacgatccg cgcaaggacg gcgccacgca ctacaacgag accatcgtgc gcgcgctgga
    42961 aacggcgggc tggtcgcgtg agaacatcgg ctcgcacatc gtccgcaaca acaccatctt
    43021 caacagcgga cagaccggca tctgcggcag catgggcgcc gttttcagca tcatcgagaa
    43081 caaccacatc tacaacatct ggaccaagcg gcagttctcg ggcgccgaga tcgccgggat
    43141 caagctccac ggcgctatcg acgtcgtgat ccgcaacaac cgcattcaca atgcgggccg
    43201 cgggctctgg atggattgga tggcacaggg cacgcgcatc acgcgcaacc tgttgtacga
    43261 caacacgacc gacgaccttt tcgtcgaagt ggaccacggc ccgttcctgg tcgacaacaa
    43321 catcatgctg tcgccgctga gcctgctgga catgtcgcaa ggcggcgcct acgtccataa
    43381 cctgctggcc gggcgcattc acagccggcc cgagccgcgg cgctcgacgc cgtatcacaa
    43441 ggcgcacgcg accgcgctcg ccggcctctc cagcatcgcg ggcggggaca accgtttcta
    43501 caacaacatc ctcttcggca acggcgggcc tcctgccgct ggcagcgagg ccgacgccaa
    43561 tcccctccgc ttcggcggtt acggcctctg ggtctacaat caccgcgagt acccgctgtt
    43621 cacgggcggc aacgtctacg tcgagggcgc gcggccgtat tgcaacgaga acggcgacgc
    43681 cactgtcgaa ggcgtccggc ccgcgctcgc cgaggagggc gattccgtct acctgcgatt
    43741 cagcctgagc gccgggacgc agaaggccgc gacgcgcgta gtgaacaccg aaatgctggg
    43801 caaggcgaag atccccggtc ttgcgtacga aaacgcggac ggctcgcccc tggtcgtcga
    43861 ttgcgactac tttggcaaga agcggacgct ggccgcgccc tccgccgggc cgttcgacaa
    43921 accgggccag ggtgacctaa agttaaaggt ctggtagtct tttggccacg caaacggacg
    43981 gaatccagtt ggcgggacgt gaagcggcca cccgcctgta ttcttatcgc atcgcgtcgg
    44041 tcacggcgct gagtggcctg ctgttcggct tcgacacggc catcatcaac ggagcgatcg
    44101 tcttcctccg gcggcatttc cggtggtcgg aaatcgaaac ggaggtcgcg gcgggaagcc
    44161 tgctggccgg gtgcgcgctc ggcgcgggcg tggccggcac gctcagcgac cgcttcggac
    44221 gcaaggtggt cctgctgctg gccgccctga tcttcgcgct ctcgtcggtg gcgacggcgc
    44281 tgcccaacgg gctcgcacag ttcgttgccg cgcgcgtggt ggccggcatc gccatcggca
    44341 tcgcgtccat gctggccccg ctctacatcg ccgaggtgtc cccggcggcc atccgcggcc
    44401 gcctggtgag catgaaccaa ctggcgatcg tcctcggcat cctggtttcg tacctggtgg
    44461 gctgggctct ggccggtttg ggcgatgaaa gctggcggtg gatgttcgcc gtggccgccc
    44521 tcccttccct gctctttttc gccgccctgt tcttcgtgcc cgagagcccg cgctggcttg
    44581 tgaaagaagg acggcacgac gaagcggcgc gcgtgctggc gcgcctgggc gagccgcctg
    44641 cgcggttagg cgagatcgag cgggccatcg ccgaagagag cggatcgctg cggcaattgc
    44701 ttgaaaaggg cctgcggcgg ccgctcgcca tcggcgtgac gctggccgtc ctgcagcagg
    44761 tgacgggcat caacacggtt ctctactacg ggtcgatcat ctttaccgaa caggcgggcg
    44821 ccgccagcac ctccgcagcg ctgttggcga acgtcctgat cggcgccatg aacctggtgc
    44881 tcacggtggt ggcgctgttc accatcgacc ggctcgggcg ccggaccttg ctcattacgg
    44941 cggcggcggg catgggcgcc gcgctcacgg cgctcggctt cgtcttcgcc gccggctcag
    45001 tgacgcccac gcccgtcctc acgctgattc tctgctacgt cgcgttcttc tccttcggga
    45061 tgggaccggg cgtgtgggta gtgatttcgg agcttttccc cacgcggatt cgcggacgcg
    45121 cgatgtcgct ggccacggtg gcgctgtggc tggcctgcct gctgatcaca ctgacgtttc
    45181 tttcgctggt gaaagcgttc acggcggccg gcgcattctg gatctacgcc ttcctgtgcg
    45241 ccgctaccgt ctggttcgtc tggaagtata cgccggagac gaagggcaag aacctcgagg
    45301 agatcgaaca gcaatggaaa tcgtgagtcc aaccgccgtt gccgccgata caagcgcccg
    45361 ccgcctcgcc gccgcgctcc gtcacgagcg cccggaccgc attcccatcg acctcggaag
    45421 caccgcggtc accggcgtcc acgtcacctg cgtggccgcg ctgcgcgact actacgggct
    45481 ggagaagcgc ccggtgaagg tgcacgagcc ttaccagatg ctgggctgga tcgacggcga
    45541 tctggccgag gtcctcggcg tggacaccgc cggcgtgttc tcccgcgaga catgttcggc
    45601 ttcccgaatg aagactggag ggagtggaat tttcgcggcc tcgacgtcct ggtgccgggg
    45661 aagttccacg tcatcgaaga ggcgaacggg gacatcctga tttacccgaa gggcgaccgg
    45721 tccgccgctc ccagcgggcg catgccggtg ggcagcgcct tcttcgatac catcatccgg
    45781 cagccgcctt tcgacgaaaa caacccggac ccggcggaca acctggagga gttcggcccc
    45841 gtcaccgagg cggacctgaa ccacctggag cgcagcatcg cctgggcggc ttccaccggg
    45901 cgcggcatcg tcgccacctt cggcggcacg gccttcggcg acatcgcgct ggtgcccggc
    45961 ccgttcatga ccaacccgaa gggcatccgc gatatcaccg agtggtacgt ctcgacgcgg
    46021 tcgcgccggc cgtacatcca ccagatcttc gagcggcaat gtgagatcgc gcttgcgaac
    46081 ctggcccgca tccgcgagcg tatgggcgac gccgtgcagg cggtcttcgt ctgcggaacg
    46141 gatttcggaa cgcagacctc ggccttctgc tccgatgcga ccttccgcga gttatggctg
    46201 ccttactacc ggcgcgtgaa cgactggatt cacgcccata cgccgtggaa gacgttcaag
    46261 cactcctgcg gcagcgtggt gcgcttcctc gacagcttcg tcgatgccgg gttcgacatc
    46321 ctgaacccgg tgcaatgctc ggccgccggc atggatccgg agatgttgaa ggcgaagtac
    46381 ggcgaccggc tcacgttctg gggaggcggc gtcgacacgc agaagacctt gccgttcggc
    46441 tcgccggacg aggtgcgcga agaggtgctc cgccgttgcg agatcttcgc gagcggcggt
    46501 ggattcgtgt tcaacaccat ccacaacatc cagtacggca cgccggtacg caacatcgtc
    46561 gccatgctgg acgcggtgcg cgagttccgc tgagggacgc gcgccgggcg cgtatatgcc
    46621 gaaatggcga ttgccgtgtt cgcgcgatcg atgtggccat ccagggcgat acgcagaagc
    46681 tggtggaagc tatgcgcggg ttccactgga tgacctcgta atggcggcca tctcagcaag
    46741 gtagagtacg ccgtgaaaaa agccggcgtg agctgggtga aggtttagcc ttcgatcttc
    46801 accttgaatt gcagcagcgc cgccgccgta tcgatcgccg cgcgatgatc gccgtagtag
    46861 gtgacgcggt gccacccggc gtcccagtcc tcggccagct tgaaggcatc gcgcggctcg
    46921 gccgcgagct tggtgcggca cgccttgtcg tcgtcgatgt tctccatggc cttggcctgg
    46981 tgcatcacca cttcgcggcg gccgggcagg aactcgagcg tggtggtcat gacgcctacg
    47041 ggcatcaggg agcgcacggc ggcgcctttg cggtcctcgg agtggctccg gatgtggtat
    47101 tcgttggacg ccttgccggg gccccacacc ttgctcggtg ccacacagtg ggcgtagatg
    47161 atgcgattgc gcgcggtgtc gatcaccggg tcggaaatgt agccgggctg cccaatcagg
    47221 tggctcatca gcagcatggt ggatgccgac gtcaggtccg cttcgcaggc ccccacccgg
    47281 ttctcgttgt tcaactggaa gaagcccagg cagggatagg cgcgcagctt gttggcgtag
    47341 aacaggtgca ggcagtccac cgtgatcgcc cgcgagcgtt gcgccgccag gagattgcgc
    47401 atggcgacat acatgcggcc gccctgcaga atttcgtcgg gggtgggctc cacgatcttc
    47461 ccggcgtctt tggtccaggt gcgggcccac tcggctgcct cggccggcga agccttgtcg
    47521 tacgccgcgt tcagctcgtc gccgctgacc ttgacgacct cggtgccgta agcctcgcgg
    47581 atctgcgcgg tcagcgccga cgggtcgcgc ccggtgacgt cgagcagctt ctcccctttc
    47641 atctgcttca cggcgtgaag cgccctcagc gcctggatca cgtcgctgaa gcggctggag
    47701 gtgacgccgg ccaccggcag cccttttcgg cgcgccggac catacgtgcc caggaaatcg
    47761 ccggtcccgc cgtagagatg gtccacgatg atggccgggc ggcccgagaa ggcgatctcg
    47821 cgccccgcgc cgccgctcgg gatcccgaga aggaagacta cgtagccatc ggtgctcgcg
    47881 gagctttcga ggatcttctt cgactcctcg gcgctcatcg cagtcgccgt ttcgaagacg
    47941 gcgaagctcc cggcaggctt cagttgggcc aggatctctt tcttcttcgc ttcgtagtcg
    48001 aagttcacgt aaggccagcc gggtttgtcc gccggcgcct gcgtgaagac cacgcggatg
    48061 ttggtcctgg ggctctcggg cgcgggcggc gccgcgcaga gagcgctcga gccgcaagcc
    48121 gctgcgcagc cggcgcaggc gcgcaggaag gcacggcggg tcgcagaagg cacgatgggg
    48181 aggtcagcca tcataatctc cttttcgggc ctgattatac tgctaagtgc cgggctcccg
    48241 gtagctctcc gcgagataga atacaacgca tgaaactgtc cgctgcgatt ctcctttccc
    48301 tgggcgcgct ggcgcagccc cctccacctg tgatgtcgcc cgaggcgcat gccgacgggc
    48361 gggtgacctt ccgcttccgc gctcccaacg ctaaagacgt gctgctggcc cgcgacggcg
    48421 cctcgcgcgt gccgatgcgg caggacgagc agggcgtctg gagcatcacc accgaggcgc
    48481 tcgagccgga cttctatgga tataccttcg tcgccgacgg agtcagcctg ctggacccga
    48541 tgaatccgcg gatgaaaccc aacctgctga acacgcagag cgtggtgcac gtgccgggac
    48601 cggcgtcgct cgtgtgggag ttgggcagca gtgcgccgcg cggcacggtg caccggcatt
    48661 tctaccggtc cgccgtggtg ggcgatgacc gcgacttcta cgtctacacg ccgccggggt
    48721 acgatccgac ggcgcacagg acgtacccgg tgctctacct gctgcacggc ttcagcgacg
    48781 atgcgagcgg ctggaccgcg gtcgggcgcg cgaacgtgat cctcgataac ctgatcgcgc
    48841 agcgcaaggc gaagccgatg ctcatcgtga tgccgctcgg ctacggcgcg ccggagattg
    48901 tgtcacgcac gcccggaccg ggcagggcgg gactgcgcca gcgcagcttc gataagttcc
    48961 gcgaagcgtt gtttaccgaa gtgatcccgg aggtcgagaa gagctatcgc gtggccaagg
    49021 accgcacggc gcgggccatt gccgggctgt cgatgggcgg cgccgaatcg ctctatacgg
    49081 gcctgaatgc ggtcgaccgg ttcgcctgga tcggagcgtt cagctccggc ggcatgggcg
    49141 acgatctcga gaaatcgttc gcgcatctgg atgcaaaggc cggcgcgcag ttgcggctgc
    49201 tgtggatcgc ctgcggcaag gaggaccggc tgattgaatc gaaccggaag ctgctcgact
    49261 tcctccaatc gcgcggcgtc cgctacacct ggagagagac gccgggcgcg cacacctggc
    49321 tggtctggcg ccggtatctg gccgaattcg cgccgctgct gttccaggac aagacctcgt
    49381 aaacgcctga cgccttcacg tccgcgcacc gcgaggactc gcgtcacgaa gccgctgacc
    49441 tgcacttcga gcgtgccgcc ttcgacggaa agcgcggcca tggccggatg cggcgagaaa
    49501 ttcgtggcca ccagcatgcc gtcgacgttc agccaatcgc gcccgcgctc gatgcggcgc
    49561 ggcgcgtggg tccatacccg ctgcggctcc tcgagcagtg agatcatgcc gccgcggagg
    49621 cgtaccgctg aggagtggct gaaatgggtg acattatgcc gggtcacaat ctgattccgc
    49681 tcgttgggca tccggcgcac cgacccgagc aatgcgcggc gttcctgccc gtcggagcac
    49741 attgtcaggg cgatcggcgg cccagttata cagcatcaca gctccgcggc tctgggctag
    49801 caagcaaggc ttactcaacc cgtaaactcg ccgcttaccg ctcggagaga gatgagaggc
    49861 gtcagccagc cgcgagcgcc aagccggcca tcgcaagaac ttccagacgc aacatggaat
    49921 tcatacggcc acgttccgca gtgccgtggc gaattcgtaa tacccacagc gctcgatttc
    49981 atcccacatg gcaagaacat tctcgacttt tacggccggg tggatctcgg tgctggaccc
    50041 cagccacaat cggccgcatc ctcccgcgcc ggctatggcc cggcgcacgg ctgccctcac
    50101 ctgatcttcc ctaccgaacg gcagcagttg agtggcatcg atgcccccca tcaggatcca
    50161 attcggacat gctggcgcga cagcttccac ggacatcccg gagagcggtt cgagcgggtt
    50221 caacccgtca atgccgctgg cacggaaatc gtcaagcacg ggccacaggt tgccgtccga
    50281 atggaagatc accttgattc cgtgcgcatg ccatgccgcc acgagcgagc gtaaccgcgg
    50341 gaaaaactcc cggcgcagga aggccggcga gaagagcggt ttgttcttgt cggcaatgtc
    50401 cgcgtatacc agcgccacgg gggagagccg cggatcggcc tcctcgtgaa ccctctgtat
    50461 ttcggcccaa ttcagtgctt ccagccattc cgagaccaag gcgggcgcct tatgcttccc
    50521 cgtccccagt gcgctcccca ttgcccggtc aatcctggcg gattcccttc cagtcacgcc
    50581 cggcctccac acggagaaac gggatgacat tattcacgct tggatgtaaa tccctctgta
    50641 ccagttccgc aaccgcgctg cggacatcgt cgcgaagcgc cgcacgtctc tgctctcgga
    50701 gcgcgtcgcg gcgtgcaacg aagccagaga cgatctgttt gtagagaact gggaaatgag
    50761 tgtagagaaa catctgcctg acaccggttg cacggcaaac gtcaggcaca ctcgcacctg
    50821 gcatttcggc agcggcggtc tcgatcttgc gccggatcgc ttcccactcc ttcgctcttc
    50881 acgctttcct ggcctcgagg agtcgctggt agagatcagg aaaataatac tccagatacc
    50941 gatggctgca gcctaatcgt cgcgcgagcg cggcggtcgc aggtggcggt tcttccgaga
    51001 gggcgctgat caacccagct cggaagcgtg cccgccgcat cgctctgctc tggagttctt
    51061 tgcgcgcaat tgctctacaa agattcggaa accgagatgt cagcgcacag gtgccttggt
    51121 atccgaattc catgcacggc agatcgcgca cttgccgctc gctcacgtcg tgcgccgctt
    51181 ccagcttgcg gccgcatccc gagcaaacca gcttccggtt tccacgcttg cgtcgtatcc
    51241 agagcttgag ggtcttggcc cgctcattga tctcgtgtcg gtggacccgg tagcctggcc
    51301 agccgagcag tttcgtccgt tcgctgtctc gcaaactgcc gagtgtgccg gaattgcctc
    51361 cgccatcaaa gctcgccctt cttggcgtcc gtcagattcc tgcgcagagc ccaaaaacga
    51421 aaggcagatt atgcagacca attcacagcg ctgcctcacg cccggatcgt gtggttgcaa
    51481 atgtgcttca ggcaagagaa ccgccgaagt atactcgaat tgcattgctc gcgaacagtt
    51541 tgtcctggtt ggaaggggac tctttagaca cagagcgctc cacaatgttc aaccattgac
    51601 cgtagcttgc cgccagtgtg caaaccggcc agtcgctgcc gaacataatt cgatcccagc
    51661 cgaagacatc gattgcgtgc tggatgtatt gcgagtacgt ctgcggattc aacacagtgg
    51721 aatccgcttc cgtcatcagt cccgagatct tgcagagtac attcgagttg gcggcgaggg
    51781 cttccagatc cctgcaccac gacgtggacg gtgttccgct cacgcggggc ttgcccatat
    51841 gattcagcac gaacgttgtc tcggggcaat tcccggcaag cacgcgaacg tgcggaagca
    51901 tctcttgccg gacgactaca tcgaaaggca agccggcggc gccgacactt cgtacgcctt
    51961 taactacttg cttcgagcag atcagatccg ctgcgccatg ctcaaaaccc tggcgaatgc
    52021 cggccagaac tagacccaaa acgttccgga gccattcgat gtaacctgcc gcagcctctg
    52081 tgccgaccgg cgcccatgcg acgatccggg tcggaattcg ggctgtccta atagctctta
    52141 gtaaccactc ggtctcgctt cgcccatctg ctcccgccga tgccgcctcg acgcatacgg
    52201 acgcggcgat atccggtggc gctattgatg caagctgctc gggaagaaaa cgtcctctca
    52261 gctcgggaat gcgttccagc cgctcgttgc gaacggcggc agtgtcccaa taatgctgat
    52321 gcgcgtcgac aacgatcatt atgggctctt agtggccccg ggcggtcagg ccaaccgccg
    52381 gcgtccccgc ggcgagggac ttcaacgcat cctcggcatg attgagcagc cggtcaatat
    52441 cgtcatccgt atgggctccg ctgacatgca ggcgcttata tgcgaggggt aggacgagtg
    52501 acccctgctc cagcaaccgt ctccggaacg cgacgtccgc ggcgacatcg cagtcgagca
    52561 gttccgtata gttgtctgca cccggttcct tgaaatacaa caccacgacc gatccgaagc
    52621 ccgtcgccca tgcgcgaatt ccgaactggt ccaccagagc ctgcagccca cgcttcaacc
    52681 gttcagcaag ccgggcggtg tgcttataga acccgcttgt ctccaacttg cgcatggttg
    52741 ccagcgcggc ggcaatcccg gcaggatgag cgttatacgt accagcaaag aagacatcgc
    52801 ctcccgcagt agcaaaccgc tgcatataat cccgctttcc gcataaagcg gcacacggat
    52861 agccattcgc catgctcttt gcgagtgtcg tcaaatcagg tgtgataccg caaatcgatt
    52921 gatatccgcc aagagcgtgc cgaaagccgg taatcacttc gtcaaatatc agcagcgagc
    52981 cgtttcgctt tgtcaggtcc cggagagcgt caaggaattc cggccggggc atgacgcaac
    53041 cgatgttgtg cgggatcggt tccaggatta cagccgcgat ttcgttactg aagtctacga
    53101 atgtctgttc cagctcagga accgagttga acgtaaggac gatcgtgttt tccaacgcct
    53161 cgggcagcat accggcggac ccgggatcgt acgtgccgat cctgtcacgc gggctgatca
    53221 cattcataca cagatagtcg tgccatccgt ggtagcagcc ctggaatttg acaagtttct
    53281 ttcgtcccgt caccgcacgg gcgaggcgga ccgcgttata agtggcctcc gatcccgaat
    53341 tgcagaagtg taccatctcg gcggaaggca cgtgcctgct gatcgtctcc gcagcttcga
    53401 tctccggctc cgttgttcca aggcccatca aatcgatttc tcgcgtcgct tccgccacgg
    53461 atctgtcgac atcggcatca cagtgaccaa gaatgatcgg accgaaggcg gcgtgatagt
    53521 ctacgtactc tttgccttcc acgtcccaga cgcgcgagcc cgaagctcgt ttgaagacga
    53581 tcggcggatt agtccggcga gtgctggaat ggacacctcc cggaacggtc cggagcgccc
    53641 gctggtcgag tgtgcgcgat gttgggtgcg cgcgatcagt attagacata ttcgtgcctc
    53701 aatcagtcgt tataccgagt tcgctgagga tgttccggtt cagaatctca tcgaagacgt
    53761 ggccgggaat cctgggcagg ttggtgcctt cggcgtaccg gttcagttga cgcagcgccg
    53821 tgatggcctg cgccgggtcc cacaacgggt aatccgatcc gaataatagc ttatcggtga
    53881 ctccccactc gaggcagacg atcatcgcct catacccctg ccatggcctg tgccaaattc
    53941 cagagatatc ggcataaacg tttgggtgct tgcgaatcac catcgcggca tcccgctgcc
    54001 agggatgtcc caggtgcgcc atgatgattc gaaggtccgg gaacacgatt gccaattcat
    54061 cgatctgaag cggaagcgaa tatcgcagca ggcctttcga ggcgggagaa gtgcccgtgt
    54121 gcgtcaggat cggtagtttc agttcggtag cacgttggaa gaacggatgc aacgctgggt
    54181 cgggcggatt gtatagcgac ataaccggat acagcttgat ccctttcatt ccgaagtcgc
    54241 ggactccacg ctccatctct tcaatggcgc cgggagagga agggtccaga gccatgaacc
    54301 cgatgtactt gcgccggttt cgctcgcaga gtctcgccac gtactcgtga ggcgtatgaa
    54361 tcccgagagc tagcgcagta atgccgaaga cgatcacccg atcgaattgc ttgacggccg
    54421 attcaaactg ctccgcagaa atctccggcc aagcgccggt gccacctctc gccgcccact
    54481 cctttatgaa atccgggccg aagtgggccg gctgcatgaa atgggtgtga tagtcgactc
    54541 gcatgcttca cgcacctgct ctctgcatga cagctccgag tgaatcggca aactcatatt
    54601 tccggcgcag gtccgagtcg agcgtgacac ccagccctgg cgaatccgat ggatac
//
LOCUS       DASTMK010000300        13275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_495284, whole genome shotgun sequence.
ACCESSION   DASTMK010000300 DASTMK010000000
VERSION     DASTMK010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13275
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495284"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFQ79_13020"
     CDS             <1..242
                     /locus_tag="VFQ79_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140635.1"
                     /translation="IGSLMLLEHRRDPWAEEMFGRMYRFVQSKFPLRQYGFPLWINFT
                     DRKVTFERHYNRVENYHHPRHLMLNLLALKRMMEA"
     gene            complement(252..1055)
                     /locus_tag="VFQ79_13025"
     CDS             complement(252..1055)
                     /locus_tag="VFQ79_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU0140636.1"
                     /translation="MNVTLHVWRQKQGAGKGRMVRYEARNVSEDMSFLEMLDVVNEEL
                     IGKGEEPIAFDHDCREGICGMCGFMINGVAHGPVPGTTVCELHMRHFKDGDELYIEPW
                     RARAFPVIKDLVVDRSAFDRIIAAGGYISVSTGSAPDGNAILVPKEKTERAMDAAACI
                     GCGACVAACPNAAAALFTGAKIAHLAMLPQGAPERPQRSLKMVLQMKEEMFGHCTNIG
                     ECEAVCPKQISIEVIARMNNDFIRASWGEREAPYRTDQPAEEPLAGGRR"
     gene            complement(1055..2968)
                     /locus_tag="VFQ79_13030"
     CDS             complement(1055..2968)
                     /locus_tag="VFQ79_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEU0140637.1"
                     /translation="MELDAKIPSGPIEGRWERHRFNLKLVNPANKRKYNIIVVGSGLA
                     GGSASATLAELGYNVHCFFFQDSPRRAHSIAAQGGINAAKNYQNDGDSIYRLFYDTVK
                     GGDFRSRESNVYRLAEISLNIIDQCVAQGVPFAREYAGTLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALMRQVEAGKVKLYPRMEMLDLVVIDGRARGIVTRNLITGEIRSHI
                     ADAVVLGTGGYGNAYYLSTNAKGANATAIWRAYKRGALFANPCFTQIHPTCIPVTGEY
                     QSKLTLMSESLRNDGRIWVPLKKGDKRAANEIPHEERDYYLERRYPSFGNLVPRDVAS
                     RNAKAICDEGRGVGESGFGVYLDFADAIHRLGRHTIEERYGNLFDMYQSITGEDPYST
                     PMRIYPAIHYTMGGLWVDYNLMSTIPGLYVIGEANFSDHGANRLGASALMQGLGDGYF
                     VLPYTIGDYLAGTKLDKTGTDHPAFQEAEANVRCITDRLLKVNGTRTVDSFHRELGKL
                     LWEYCGMSRTAEGLKYALGKIPELREQFWKDVKVLGGGKELNQSLEKAGRVADYFELG
                     ELICLDALARNESCGGHFREEYQTAEGEALRNDQDYSYAAAWQYRGEGMEPALHKEHL
                     EFEYVHPSQRSYK"
     gene            complement(2970..3674)
                     /locus_tag="VFQ79_13035"
     CDS             complement(2970..3674)
                     /locus_tag="VFQ79_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEU0140638.1"
                     /translation="MSAIAVDLKVKHGFRFYESTIGKKVVMAVTGFILFGFVVGHLIG
                     NLQVFGPPEKINNYAVMLRAVPAALWAARIVLLLSVILHIVASIQLTKLKWDARPVKY
                     QSKESVGSTYASRTMMWSGPIIAAFVIYHLLHFTFGWRVVSPHFDQHNVYNNIIYGFQ
                     FWPASLFYIVAMVLLGLHLYHGVWSMFQTLGVSHPRYTPWLRRLALAFALFVAGGNIL
                     IPVAVLTGYLRPVTGV"
     gene            complement(3712..4695)
                     /locus_tag="VFQ79_13040"
     CDS             complement(3712..4695)
                     /locus_tag="VFQ79_13040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0140639.1"
                     /translation="MSIKGLAAAVSWLFWGRVKHTLRRYRNVKELGNALLRGEEYPNP
                     SDHLVMERRFPLDTEGRQEIEVKTCEATLHRLLDQVQRSWKKLGEEVPHWSVITSNQF
                     KPDQFDAHEEEFWRSGEGDVQRFFIWAARNSIPVDPGWTCLEYGCGTGRLTAVLAQRF
                     SKVIACDVSAPHMALAARKLKDQGNTNVEFVPVNSLAALDSLPSVDLIFSVIVLQHNP
                     PPVIAALLDRLLTRLRPGGVAYFQVPTRARGYTFKVDDYLRDRKDKLEMEMHVIPQKD
                     VFEILRRADCELIEVQPDNMTGSLDFISNTFLVRKLSAGGGEVPAADFRIQ"
     gene            4819..5427
                     /locus_tag="VFQ79_13045"
     CDS             4819..5427
                     /locus_tag="VFQ79_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU0140640.1"
                     /translation="MIKAVIFDLGGVIVPLDFAQGYTAISKFCSYPAAEIPQRLASTD
                     LITRFESGQIEPEKFAEQLSGILGLKIDYDEFCAVWSSIFLPHTLVSEELLDRVKRHH
                     RLLLLSNTNAIHFEMLRRTYPILGRFDGLVLSYEVGAMKPSPRIYREAVARAGCRPEE
                     CFFTDDVLAYVEGARKEGIDAVQFESREQIERELRSRGVDLD"
     gene            complement(5424..5816)
                     /locus_tag="VFQ79_13050"
     CDS             complement(5424..5816)
                     /locus_tag="VFQ79_13050"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0140641.1"
                     /translation="MRRPGPRRDIPPPLELECLKVLWSTGESNVKTVRDALKKNRVLA
                     YTTVMTVLDRLAKRGGVSRRKIGRAFVYSPLLSREALRKAAVSELVESYFDGSEDALV
                     KYLTESDTATPPVTAPEWQEERLDTVLL"
     gene            complement(5818..6936)
                     /locus_tag="VFQ79_13055"
     CDS             complement(5818..6936)
                     /locus_tag="VFQ79_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU0140642.1"
                     /translation="MKSAFARELQPNQAVTTTFLVQTKDIRQKKTGEPYLSLQLGDRT
                     GDIDAKMWDNVAEIMHTFDRDDFVKVKGVLQIYQNRLQLNIHKMIKVDERDVDVRDYF
                     PCSKRDPDEMFRELEEIIASVANPHLRALLEAFFSDADIASRFRVAPAAKSIHHAYLG
                     GLIEHVLSLCKLCRLVAPHYPDVDPDLLLTGAILHDVGKISELTYARSFGYSNEGQLL
                     GHIAIGLRTLGSKIETVPGFPPALRLLVEHMILSHHGELEFGSPKVPLFPEALLLHHL
                     DNLDSKMECMRAFTERDARLDGCWTAYNNALERVVLKKARYLEGESTQSAPEAPKTEA
                     VAANGSAMPGTAPPVPQERPSSVESPFAQKLQRALGKD"
     gene            complement(6954..8087)
                     /locus_tag="VFQ79_13060"
     CDS             complement(6954..8087)
                     /locus_tag="VFQ79_13060"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU0140643.1"
                     /translation="MKFLRFLLVTGVVSVSAAAADAVLVDEIIAKVNGEIITRSELER
                     TRRQMEGELKQHGATGAQLKQALEERERDLLRDRIDQMLLVQRAKQMDINVDAEVSKY
                     LAEVQLRTKIAEPEKFHDFIRQQLGMPYEDYRSEIRNSMMTQRVIRQEVGDRIRPPRS
                     DLEKYYNEHKDEFVREERIFLREILVSTEGKDAAGVAAAEKKAKDLVARARKGERFGE
                     LARDNSDAESAEQMGELPGWEKGQLDKELEAIVWDQERGYVTDPIRRPNGFLILRVEE
                     HHKAGLAPFEEVENEIMEKLYMPLFQPRVREFLTRLRTEAFLEIREGYVDTSAAPGKD
                     TSWSDPAELRPETVTKEEVLNQPRRKRLLWLIPIPGTKSTPEP"
     gene            complement(8109..11597)
                     /gene="mfd"
                     /locus_tag="VFQ79_13065"
     CDS             complement(8109..11597)
                     /gene="mfd"
                     /locus_tag="VFQ79_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU0140644.1"
                     /translation="MIHPAVRDLFQSLSRQPAFQELAHALAAGPGPEHAISLSGLTTT
                     AKALYIVLLRQATERPVLLLVDGNKQAEAYEELIGTFYEILISSRASEAPQLIPALDV
                     LPAQKLSPHSEVSEQRAIGLWRIATRGVPIAITPVGSALLRTYNAEFYRQLPLLLRTG
                     DELPLDDILSHLHSIGYERREPVEMVGEYSLRGGILDVFPAQAQKPVRIEFFGDSIES
                     MRRFDVESQRSVVKVQDCLLLPLLEEPRSRDLLKEIAARLPEGAAVVSGETFPGWEFA
                     APLVRARTSSILSLMDNPIVIWDEPEQIRGAAERLWTRLEERDQEAYASSTAIYYKFS
                     DFESEARNKSQVHLRQLELGGSGLHIPSRPSLAFRGNLPVAVAETRSLVETGQRVVFF
                     ASSMGELERLADIFQEYSVPFQVGMEQSGSTPEYLAERAYMAGSLASTFLVRGHVPRG
                     TILPDSGLAIFGSEDLFETSDLVVRPGGLRSHAAAFSTDSMDLSPGDYVVHAEHGVGR
                     FAGIREIEHGDQKGDFMLLEYAGETKLYVPMTRMDLIQKFRGAGDSPPTLDRLGGATW
                     NRTKSRVKAKIRDMADELLKLYAERKMAEGFSFSPDSNWQRELEDAFEFAETKDQVTA
                     IRDIKRDMESAHPMDRLLCGDVGFGKTEVAMRAAFKALGDGKQVAVLAPTTVLAFQHH
                     ETFKRRFASFPVRVELLSRFRMPKEIAEVIGDVANGKVDVVIGTHRILSKDVEFRDLG
                     LLVVDEEQRFGVRHKERLKQLKKNVDVLTMTATPIPRTLHMSLLGMRDMSVIETPPKD
                     RLAIQTVVAHYHPELIKTAIEQELGRGGQIYFIHNRVDTIWSRAASIQELVPQCRIGV
                     GHGQMGEAELEKVMLGFMRYEYDLFVSTTIVENGLDIPLANTIIIENAERYGLAAMYQ
                     LRGRVGRSNRRAYAYLLVPPDSELSEVARKRLAALKEFSDLGAGFKIAALDLELRGAG
                     NLLGGEQHGHINSVGFDMYVRLLEETAREMKGEEVPLEIHSSLNLGLDLRIPPGYIAD
                     EHQRLRAYKKIADITDSGHAAKVLAEMEDRYGPVPGQVKSLAEFALIKSTAQRLGIES
                     IERRQGMLNIKFHKDSRVQAAALMNLVRKTAGAQFTPAGVLRIPVTSGGEASQLLGFV
                     GAQLLQLDAQESSLRS"
     gene            complement(11594..12067)
                     /locus_tag="VFQ79_13070"
     CDS             complement(11594..12067)
                     /locus_tag="VFQ79_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU0140645.1"
                     /translation="MGRIYLQEDLLDVRAEWKRAGKVVVFTNGCYDILHPGHIRLLEA
                     AKALGDLLVLGLNSDSSVSRLKGPGRPLIPERDRAEMAAALSAVDAVTLFDEDTPREL
                     ISRLLPDILVKGADWSHWIAGREEVEAAGGKVLALPLEAGYSTTGIVEEILARQL"
     gene            complement(12095..13144)
                     /locus_tag="VFQ79_13075"
     CDS             complement(12095..13144)
                     /locus_tag="VFQ79_13075"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEU0140646.1"
                     /translation="MAERSVTVSGALEKIPAFPPIAVRVLQLTSDEGVPIVQLEKAIM
                     LDPVLSTEVLRRANSALFNLTSEVTTLKHALIILGLDRIKSISTTIAASRYLKSTLIV
                     DELRACWRASIARAVIGQMLARYFLLQEDEAYIAGLLKDLGRLGLMTAYPPEYTHLLS
                     MAAASVHAEAVDIREAEIQMFSIDSASAGIYLAGKWGLPDYIQSVLSEEPGAENASNL
                     ARLVHLASRLAEALGFGLIPGAASADFETVLAEIPEYVRNRIPRNADLFRREVEARLE
                     SFDPNVKPAPMATTPLVSSPGERALKDRSSDEAVFSNTVAPLEPEHERVWIWILCCGV
                     LTTVFTLLFMMLLRR"
BASE COUNT         2656 a         4072 c         3749 g         2798 t
ORIGIN      
        1 tgatcggctc gctcatgctt ttagagcacc gccgcgatcc ctgggcggaa gagatgttcg
       61 gccgcatgta ccggttcgtg caaagcaagt tcccgctccg gcaatacggc tttccgctgt
      121 ggatcaattt caccgatcgc aaggtaacct tcgagcggca ctataaccgg gtcgaaaact
      181 accatcatcc ccggcacctg atgctgaatc tgctcgcgtt gaagcgaatg atggaagcgt
      241 gagaagagcc gctagcggcg gcctcccgcc aacggctctt cggccggctg atcggtgcgg
      301 tacggtgctt cgcgttcgcc ccaactggcc cggatgaagt cgttgttcat gcgcgcgatc
      361 acctcgatgc tgatctgctt cgggcagacc gcctcgcact cgccgatatt cgtgcaatgc
      421 ccgaacatct cttctttcat ctgaagaacc atcttgagag agcgctgcgg ccgttcggga
      481 gcgccttgcg gcagcatggc caggtgtgcg atcttggcgc ccgtgaatag cgccgcggca
      541 gcgttcggac aggccgccac gcaagcgccg catccgatac aggctgctgc gtccatcgcc
      601 cgttccgtct tttcttttgg aaccaggatc gcgttgccat ccggcgcgct gcccgtcgag
      661 acggagatgt aaccgcccgc ggcgatgatt ctgtcgaagg cgctgcgatc cacaaccagg
      721 tctttaatta ccggaaaggc ccgcgcgcgc catggttcaa tgtacagctc atcgccatct
      781 ttgaagtggc gcatgtgaag ctcgcagaca gtggtgccgg gaactggccc gtgcgccacg
      841 ccgttaatca tgaagccgca cattccgcag atgccctcgc ggcaatcgtg atcgaacgcg
      901 atcggctctt cgccttttcc aatcagctct tcattgacga cgtccagcat ttcgaggaac
      961 gacatatctt cgctgacatt tcgggcctcg tagcggacca tgcggccctt ccctgctccc
     1021 tgcttctgcc gccagacgtg taatgtcacg ttcattactt atagctccgt tgcgatggat
     1081 gcacgtattc gaactccagg tgctctttgt ggagagccgg ctccatgcct tctccccggt
     1141 attgccaggc ggcggcatac gaatagtcct ggtcgttgcg aagcgcttcg ccttccgccg
     1201 tctgatattc ctcccggaaa tggccgccgc aggactcgtt tctggcgagt gcgtcgaggc
     1261 atatcaactc gccgagctcg aagtaatccg cgacgcggcc ggccttctcg agcgattggt
     1321 tgagctcctt gccgccgcca aggactttga catctttcca gaactgctcg cgcaattcgg
     1381 ggatcttacc cagtgcgtac ttcagcccct ccgccgttcg cgacattccg cagtactccc
     1441 aaagaagttt gccgagttcc cggtgaaaag aatccaccgt gcgggttccg ttcaccttga
     1501 gaaggcggtc ggtgatgcac cggacgtttg cttccgcctc ctgaaatgcg gggtgatcgg
     1561 tgccggtctt atcgagcttc gtgcccgcca ggtaatcgcc gatcgtatag ggcagtacga
     1621 agtagccgtc ccccagacct tgcatgagcg cgcttgcccc cagccggttc gcgccatggt
     1681 ccgaaaagtt ggcttcgccg atcacgtaga gccccggaat agtgctcatg aggttgtaat
     1741 ccacccaaag gccgcccatc gtgtagtgaa tggccgggta gatgcgcatg ggggtgctgt
     1801 acgggtcttc tccggtgatg ctctggtaca tgtcgaagag gttgccataa cgctcttcga
     1861 tggtatggcg tcccagccgg tggatagcat cggcgaaatc gagatacacc ccgaagccgc
     1921 tctcccctac accgcgccct tcatcacaaa tggcttttgc atttcgtgaa gcgacgtcgc
     1981 gcggcaccag gttgccgaaa ctcggatacc gccgctccag gtagtaatcg cgctcctcgt
     2041 gcgggatttc gttggccgcg cgcttgtcgc ctttcttgag cggtacccag atgcggccgt
     2101 cgttgcgaag cgactcgctc atcagcgtta gctttgattg atactcgccg gtgacgggaa
     2161 tacatgtcgg gtgaatctgt gtgaaacagg ggttggcaaa tagcgcgcct cgcttgtacg
     2221 cccgccagat ggcggtggcg ttcgcgccct tggcgttcgt cgaaagataa taagcgttgc
     2281 cgtatccgcc cgtgccgaga accactgcgt ccgcgatatg agaccggatc tcgcccgtaa
     2341 tcaggttccg tgtcacgata ccgcgcgcgc gcccgtcgat caccacaagg tccagcattt
     2401 ccatgcgcgg gtacaacttc accttgccgg cttccacctg gcgcatcaac gcctggtagg
     2461 cgccgagcaa caactgctgg cccgtctgcc cgcgggcgta gaacgtacgc gagacctgtg
     2521 cgccgccaaa cgaacgattg gccagcgtac ccgcgtactc gcgcgcgaac ggaacaccct
     2581 gcgccacgca ctggtcgatg atattcaggc tgatctccgc gaggcggtac acgttcgatt
     2641 cgcgcgaccg gaaatcgccg cccttcaccg tgtcatagaa cagccggtag atgctgtccc
     2701 cgtcgttctg gtaattcttc gcggcgttga ttccgccctg tgccgcgata ctatgcgcgc
     2761 gccgggggct gtcctgaaaa aagaagcagt gaacgttata cccgagctcg gccagcgtcg
     2821 ccgaggcgga gcctcccgcg aggccggagc cgacgacgat gatattgtac ttgcgcttat
     2881 tggcggggtt gacgagcttg agattaaaac ggtggcgttc ccatctcccc tcgatcggac
     2941 ctgaaggaat tttagcgtcg agctccattc tatactcccg tgaccggtct caaatagccg
     3001 gtaagtacgg cgaccggaat caggatgtta ccgcccgcca cgaagagcgc gaacgcaagc
     3061 gccaggcgcc tcagccacgg cgtgtaccgc ggatggctca cgcccagcgt ttgaaacata
     3121 ctccacacgc cgtgatagag atgcaagccg agaagcacca tcgcgacgat ataaaagagc
     3181 gatgccggcc agaactgaaa tccgtaaata atgttgttgt agacgttgtg ttggtcgaaa
     3241 tgggggctca ccacgcgcca tccaaatgtg aagtgcagca gatggtagat gacaaaggcc
     3301 gcgataatcg ggccgctcca catcatcgtg cgagaggcgt aagtggaacc cacgctctct
     3361 ttactttgat acttcaccgg acgggcgtcc cacttcaact tagtaagttg gatggaagca
     3421 actatgtgca ggattaccga cagcagcagg acgatgcgcg ccgcccagag cgccgccgga
     3481 acggcccgca acatcaccgc gtagttattg atcttttccg gcgggccaaa gacctgcaaa
     3541 ttgccgatga ggtggccgac cacgaaccca aacaggatga agcccgttac ggccattaca
     3601 actttctttc cgatggtgga ttcgtagaac cggaacccgt gctttacctt tagatcaact
     3661 gctattgcgc tcatccgcgc cctctcccct gaatcgatgc ggttagaagt ttcattgtat
     3721 tcgaaaatcg gcggcgggca cttcgccccc tcctgcggac agctttcgaa ccagaaaggt
     3781 attcgagatg aaatcgagag aaccggtcat gttgtccggc tgaacttcga tgagctcgca
     3841 gtctgctctt cgaaggatct cgaacacgtc tttctgcgga atcacatgca tctccatttc
     3901 cagcttgtct ttccggtccc gcaaatagtc gtccactttg aaagtgtatc cccgggctcg
     3961 cgtcggcacc tgaaaatagg cgactcctcc cggacgcagg cgcgtcagca acctgtccag
     4021 cagggccgct atcaccggag gagggttatg ttgcagaacg atcaccgaaa agatcaaatc
     4081 gacagacggc aagctgtcga gagcagccag ggagttcacc gggacaaact cgacattcgt
     4141 gtttccctgg tctttcagct tccgcgccgc cagtgccatg tgaggtgcgg acacatcgca
     4201 cgcaatcact ttgctaaacc tttgggcgag cactgccgtc aatcttccag tgccacagcc
     4261 atattcgaga caggtccacc ccggatccac gggaatgctg ttgcgcgcgg cccagatgaa
     4321 gaaacgctga acatcgcctt ctccggatct ccagaattcc tcctcatgag catcgaactg
     4381 gtccggcttg aattggttac ttgtgatcac ggaccaatga ggaacttctt ctcccagctt
     4441 tttccacgat ctctgcacct ggtcgagcaa ccgatgaaga gtcgcttcgc aggtcttcac
     4501 ttcaatctcc tgcctgcctt ccgtgtccag cggaaatcgc ctttccatca ccaggtggtc
     4561 cgacggattc ggatactcct caccccggag caatgcgttt ccgagctcct ttacattccg
     4621 gtagcgccgt aacgtgtgtt tcacccgtcc ccaaaacagc caggaaacgg cggcggcaag
     4681 gcctttgatg gacatgctct cagatatcct gttgaaccaa aacttgccgc cggggaagaa
     4741 gcgatgcgcg cttgcttgtg ctgccgctaa actggtctgg tggcgccggg gtaagcttcc
     4801 ggtacagtac acaattatat gataaaagcc gttattttcg atctcggtgg agtgattgtg
     4861 ccgctcgatt tcgcgcaggg ctatactgct atttccaaat tctgctctta tccggcagcg
     4921 gaaattcccc agcggcttgc ctccacggat ctgatcacgc gtttcgaatc cggccagatt
     4981 gagccggaga agttcgcgga gcagttatcg ggtattctcg gcttgaagat cgactacgac
     5041 gagttctgcg cggtttggag cagcatattt ctccctcaca ccctcgtctc cgaggagctg
     5101 ctggaccggg tcaagcggca tcaccgtctc ctattgcttt ccaacacgaa tgccatccat
     5161 ttcgagatgc tgcgccgcac ttatccgatc ctgggccggt ttgatgggct cgtgctgtcc
     5221 tacgaagtgg gcgcgatgaa gccgtcgccg cggatttacc gggaggctgt ggcgcgggcc
     5281 ggctgccgtc ccgaagaatg cttctttacc gacgacgtgt tggcatacgt ggaaggcgcg
     5341 cgaaaagaag gcatagacgc ggtgcaattt gaatcgcggg agcagattga gcgcgagttg
     5401 agaagccgcg gcgtcgatct cgactaaagc aagaccgtat ccagccgctc ttcctgccac
     5461 tccggggcgg tgaccggcgg cgtggcggta tcggattccg tcaggtattt cacgagcgca
     5521 tcctcggaac cgtcgaaata ggactccaca agttcgctga cggcggcttt gcggagcgct
     5581 tcgcggctca acagcggcga atagacaaag gcgcgtccga tcttgcggcg ggaaacgcct
     5641 ccgcgtttgg ccagacggtc cagaacggtc atcaccgttg tgtaggcgag cacccggttc
     5701 ttcttcaagg catctcgcac cgtcttcacg ttcgattcac cggtgctcca aagtaccttc
     5761 aggcactcga gctccagggg aggaggaatg tcgcgtcgtg gccccggcct ccgcatctca
     5821 gtctttcccc aatgcccgtt ggagtttctg cgcgaacggc gattccacgg aagacgggcg
     5881 ctcctgaggg accggaggcg ccgtgcctgg catcgccgag ccgttagcgg caaccgcctc
     5941 cgttttggga gcctcaggcg cggattgcgt gctctctcct tcaaggtatc gggctttctt
     6001 cagcaccacg cgttccagcg cgttgttgta cgcggtccag cagccatcca ggcgggcatc
     6061 ccgctcggtg aacgcgcgca tacactccat cttcgagtcg agattgtcga ggtggtgcag
     6121 caacagagcc tccggaaaaa gcgggacctt cggagatccg aactcgagtt ccccatggtg
     6181 gctcaggatc atatgctcca ccagcaaccg cagggccggc ggaaagccgg gaaccgtttc
     6241 aatcttgctg ccgagagtgc ggagcccgat cgcaatatgt cccagaagct gtccctcgtt
     6301 gctgtagccg aagctccggg cataggtaag ctcggagatt ttgccgacgt cgtgcaggat
     6361 cgccccggta agcagcagat cgggatcgac gtccggatag tgcggagcga ccaggcggca
     6421 gagcttgcac agcgacagta cgtgctcgat caatcccccc agataggcgt ggtggatcga
     6481 tttcgccgcc ggagccacgc gaaaacgcga ggcgatgtcc gcatcggaaa aaaacgcctc
     6541 gagcaaagcg cgaagatgcg ggttggcaac gctcgcgatg atctcttcca gctcccgaaa
     6601 catttcatcg ggatcgcgtt tcgagcacgg aaagtagtcc cggacatcca catcgcgctc
     6661 gtccactttg atcatcttgt ggatgttgag ctgaagccgg ttttgataga tttgcaggac
     6721 gcctttcact ttgacgaaat cgtcgcggtc gaaggtgtgc atgatttcgg caacgttgtc
     6781 ccacatcttg gcgtcgatgt cgcccgtcct gtcgcccagt tgcagggaca ggtaaggctc
     6841 acccgtcttt ttctgccgga tgtcctttgt ttgaaccaga aaggttgtgg tgacagcctg
     6901 attcggctga agttcccgcg caaaggcaga tttcatggct cgtcagtcta cggtcagggc
     6961 tccggggtgc tcttggtacc cggaatcggt atcagccaca gcaggcgctt tcgtctcggc
     7021 tggttcaaga cctcctcctt cgtcaccgtc tccggtctca gttcggctgg atccgaccag
     7081 cttgtatcct tgccgggcgc cgcggaggtg tctacatagc cctcccggat ttcaaggaaa
     7141 gcctctgttc gcaaccgggt cagaaactcg cgaacgcggg gctggaagag cggcatatac
     7201 aacttttcca tgatctcgtt ctccacttcc tcaaaaggcg ctaatccggc tttgtggtgc
     7261 tcttccacac gcaggatcag aaatccgttc ggtctgcgga tcggatcggt tacatagccc
     7321 cgctcctggt cccacacaat ggcttcgagc tctttatcca actgaccctt ttcccaaccg
     7381 ggcagctcgc ccatctgctc ggcggattcg gcatcggagt tgtcccgggc cagttcgccg
     7441 aagcgttccc ccttgcgtgc gcgtgccacc aggtctttgg ccttcttttc ggcggcggca
     7501 acaccggccg cgtctttgcc ttccgtggag accagaatct cccggaggaa aatgcgctcc
     7561 tcccggacga actcgtcctt atgctcgttg taatacttct ctaaatcact gcgcgggggc
     7621 cggatccggt ctcctacctc ctggcggatt acgcgctgcg tcatcatgct gtttcgaatc
     7681 tctgagcggt aatcctcata cggcatgccc agttgctgcc gtataaagtc gtgaaacttc
     7741 tccggttcgg cgatcttcgt tcgcagttgc acctcagcca ggtacttact gacttcggcg
     7801 tcgacgttga tgtccatctg cttcgccctt tgcaccagca gcatctggtc gatacggtcg
     7861 cgcaagaggt cgcgctcccg ctcttcgagc gcttgtttca actgggcccc ggtcgcgccg
     7921 tgctgcttca gctcaccttc catctggcgc cgggtacgtt ccaactcact tcgcgtgatg
     7981 atttcaccgt tgaccttggc gatgatttcg tcgaccagaa ccgcatcggc ggccgccgcg
     8041 gaaacggaca ccacacctgt tacaagcaag aatcggagga acttcatata gttcgattat
     8101 ccgatatttt acgaacgtag tgaactctct tgtgcatcca gttgtagcag ttgagcaccg
     8161 acgaatccaa gcagttgaga agcttccccg ccggaggtaa ccggaattcg cagaacaccc
     8221 gccggcgtga actgtgcccc cgccgttttt cggaccaggt tcatcagcgc cgccgcctga
     8281 acccgcgagt ccttgtggaa cttgatattc aacatcccct gcctccgctc aatggattcg
     8341 atccccagcc gctgcgcggt gctcttgata agggcgaact cggcaaggct cttcacctgc
     8401 ccgggtaccg gcccgtaacg gtcttccatt tccgccagca ccttggcggc atgcccgcta
     8461 tccgtgatgt ccgcgatctt tttatacgcg cgcagccgct gatgctcatc ggcaatgtag
     8521 ccgggaggaa tccgaagatc cagccccaga ttcagcgagg aatggatttc gagcggcacc
     8581 tcttccccct tcatctcacg ggcggtctct tccagcaggc gcacatacat atcgaatccc
     8641 accgagttga tatgcccgtg ctgctcgcct cccagcaggt ttcccgcgcc gcgcagctcc
     8701 aggtcgagag cggcaatctt gaaccccgcg cccaggtcgc tgaactcctt caacgctgcc
     8761 agccgcttgc gggccacttc gctcaattcg gaatcgggcg gcaccaacag atacgcataa
     8821 gcccggcggt tcgaccggcc cacccgtccg cggagctgat acatggccgc caaaccgtac
     8881 cgctccgcgt tttcgataat gatcgtattt gcgagaggga tatccagtcc attttctact
     8941 atagtagtag aaacaaaaag atcatattcg taccgcataa accccaacat caccttctca
     9001 agctccgcct cgcccatctg gccgtgtccc acccctatac ggcattgtgg caccagttcc
     9061 tggatcgagg ccgcgcgcga ccaaatggta tccacccggt tgtgtatgaa atagatctgg
     9121 cctccgcggc ccagttcctg ctcaatggcc gttttgatca gttccgggtg gtaatgcgca
     9181 accaccgttt ggatggccag ccggtctttg ggcggagtct cgataacgga catatcccgc
     9241 attcctaaca gggacatgtg aagggtgcgt ggaatggggg tagccgtcat ggtcagcacg
     9301 tccacgttct tctttagttg cttgagccgt tctttgtggc gtactccgaa gcgctgttcc
     9361 tcgtcgacca ccagcagccc gagatcgcga aattcgacat ctttcgaaag aatccggtgc
     9421 gtgccgatca cgacatccac ttttccattg gccacatcgc cgatcacctc tgcaatttcc
     9481 ttgggcatgc ggaaacggct cagcagttcg actcgcacgg gaaaggaagc aaagcggcgt
     9541 ttgaacgtct cgtggtgttg aaacgccaaa acggtcgtag gggcaagcac cgccacctgt
     9601 ttcccgtctc ccagcgcctt aaaagctgcc cgcatcgcca cctcggtctt gccgaaaccc
     9661 acgtctccgc acaggagacg gtccatggga tgagcgctct ccatgtcccg cttgatgtcc
     9721 cggatagctg tgacctgatc cttcgtttcc gcaaactcga aggcgtcctc gagttcccgt
     9781 tgccaattgc tgtcgggaga aaaggaaaag ccttccgcca tcttgcgctc cgcgtaaagc
     9841 ttcaggagtt cgtcggccat gtcgcgtatt ttggccttca cacgcgactt ggtgcggttc
     9901 cacgtcgctc cccccagccg gtccagggta ggcggggaat cgccggctcc ccggaacttc
     9961 tgaatcaggt ccatgcgggt catcggcaca tacagcttcg tctcacccgc atattccagg
    10021 agcatgaagt cgcctttctg atcgccatgc tcgatttcgc gaattccggc gaaacgcccg
    10081 actccgtgct ccgcatggac aacgtagtcc ccggggctga ggtccatcga gtcggtactg
    10141 aaggcagcgg catgggacct cagcccaccc ggccgcacca ccaggtccga agtttcaaaa
    10201 aggtcttcgg agccgaagat agccagcccc gaatccggca ggatggttcc gcggggcacg
    10261 tgcccgcgca ccagaaaggt actggccagc gaccccgcca tgtaggcgcg ctccgccagg
    10321 tattccgggg tggaaccgct ctgttccatt cccacctgga acggaacgga gtactcctgg
    10381 aaaatatcgg cgagacgttc caattccccc attgaggacg cgaagaagac tacacgctgt
    10441 cccgtttcga ccagcgagcg ggtctccgcg accgccaccg gaagattacc gcgaaaggcc
    10501 agcgatgggc gtgatgggat gtgcagaccg gatcccccca actccaactg ccgcagatga
    10561 acctgggatt tgttccgggc ttccgattcg aaatcactaa atttgtagta gatcgccgta
    10621 gaggaggcat aggcttcctg gtcccgctcc tcgagccgcg tccaaagccg ctccgcggcg
    10681 cctcgaatct gttcgggctc atcccagatg acaatcgggt tatccatcaa cgagagaatc
    10741 gaagaggtgc gcgcccggac cagcggtgcg gcgaactccc agcccgggaa ggtttctccg
    10801 gacacaaccg ccgccccttc cggcaaccgc gcggcgatct ccttcaacag atcgcgggag
    10861 cgtggctcct cgagcaatgg aagcagcagg caatcctgaa cctttacaac tgagcgctgg
    10921 ctctcgacgt cgaatcgccg catggactcg atcgagtcgc caaaaaactc gattcggacg
    10981 ggcttctgcg cttgtgccgg gaacacgtcc aaaatgccgc cccgtagcga gtattctccg
    11041 accatctcga ccggctcccg gcgctcatag ccgatgctgt gaaggtgcga aaggatgtcg
    11101 tcgagaggca attcgtcgcc ggtacgcaaa aggaggggga gttgccggta gaactcggca
    11161 ttataggttc gtagcagggc ggaacccacc ggagtgatgg cgataggcac accccgcgtt
    11221 gcgattctcc acaaaccgat agcgcgttgc tcgctcactt ccgaatgcgg ggataacttt
    11281 tgtgccggca ggacgtccag cgccggtatc agttgcggcg cctcggaggc acgggacgaa
    11341 atgagaatct cataaaacgt cccgataagc tcttcgtaag cttccgcctg cttgttgccg
    11401 tctaccagca acagcaccgg acgctccgtc gcctgccgga gcaggacgat atagagggct
    11461 ttggcggttg tcgtgaggcc ggaaagagag atggcgtgct ccgggcccgg cccggcggca
    11521 agggcgtgcg ccaattcctg gaatgccggc tggcggctga gcgattgaaa gagatctcga
    11581 acggctggat gaatcaaagc tgacgcgcca ggatctcctc aacaattcca gtggtggaat
    11641 agcccgcctc gagcggcaag gccaaaacct ttccgccggc cgcttcaacc tcttcccggc
    11701 cggcaatcca gtgggaccaa tcggcgcctt taaccagtat atcgggcagg agtctggaga
    11761 tgagttcgcg aggcgtatcc tcgtcgaaca gcgtgacggc atcgacggcg gaaagcgctg
    11821 cggccatttc cgcgcggtct cgctccggga tgaggggacg tcccggtccc tttaaccggc
    11881 tgacgctcga atcggaattg aggccgagta ccagcagatc gcccaacgcc tttgcggctt
    11941 cgagcagacg tatgtggccg ggatggagta tgtcataaca gccgttggtg aacaccacca
    12001 ctttcccggc ccgcttccat tcggctcgca catccagcag gtcctcctga aggtagatgc
    12061 gacccatagt caacttcgga ttgtaccgct gtcatcaccg gcgaagcagc atcataaaaa
    12121 gcaacgtgaa aacagtggtg agcacaccgc agcagagtat ccagatccag accctttcat
    12181 gctctggttc cagaggggca acggtgttgc taaagacagc ttcgtccgaa gagcgatcct
    12241 tcagggctct ttccccgggt gaagaaacca gtggagttgt agccatagga gccggtttca
    12301 cattcgggtc gaacgattcc agccgggcct cgacctcgcg ccggaacaaa tccgcgttgc
    12361 ggggaatacg gttccgtacg tactcgggaa tttccgcgag aacggtttcg aaatcggcgg
    12421 aagcggcgcc aggaatcaaa ccaaatccca gggcttcggc cagcctggaa gcaaggtgaa
    12481 ccaggcgcgc caggttggag gcgttctccg ctccgggctc ttccgaaagg accgattgga
    12541 tgtaatcggg aagaccccac ttccccgcaa gatagatacc cgccgacgcg ctgtcgatgc
    12601 tgaacatctg gatttcggct tcccggatgt ccactgcttc ggcgtgcacg ctcgccgccg
    12661 ccatcgagag cagatgagtg tattcggggg gataggcggt catcagcccc aggcgtccta
    12721 ggtctttcag cagtccggcg atgtacgcct cgtcctcctg taacagaaag tagcgtgcca
    12781 gcatctgccc gataaccgcc cgggcgatac tggcgcgcca gcaggcgcga agctcgtcca
    12841 cgatgagagt gcttttcaga tagcgcgagg cggcaatggt cgtggagatc gacttgattc
    12901 gatcaagccc caggatgatc agggcatgct tgagcgtggt cacttccgac gtcagattga
    12961 aaagggcaga gttggcccgc cgcagaacct ctgtcgaaag aaccggatcg agcataatcg
    13021 ctttctccag ttggacgatc ggcactccct catcggacgt gagttgaagc acgcgaaccg
    13081 caatgggagg aaaggccggg atcttctcca atgccccgga aactgtcacc gagcgttctg
    13141 ccatgcgagc accgtccgcg gctctgacta tctatcgtcc gcagaacgta aaacattagg
    13201 gatggatccg ccccgcagcc cgctcgcggc tgcctaagcc cgtctgtgcc gccggaggga
    13261 caggaggccc aacac
//
LOCUS       DASTMK010000301        31572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_430480, whole genome shotgun sequence.
ACCESSION   DASTMK010000301 DASTMK010000000
VERSION     DASTMK010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 31572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31572
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430480"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFQ79_13080"
     CDS             <1..315
                     /locus_tag="VFQ79_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0140647.1"
                     /translation="IWGGEFGRTVYCQGKLTTTNYGRDHHPRCFAMWMAGGGIKAGVS
                     LGETDDYCYNVVEDPVHVHDIQATALHCLGIDHKRLTYKFQGRHFRLTDVHGELVKKI
                     LA"
     gene            complement(331..1110)
                     /locus_tag="VFQ79_13085"
     CDS             complement(331..1110)
                     /locus_tag="VFQ79_13085"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0140648.1"
                     /translation="MVIPMLRAFLAVFVWALAPAFAQVPDLYTTESYGDPPFLSQPGW
                     RPLLNGQDLNGWHGAGRDPHGWVTTRSVIWRRIFDPKRLTFTPGPGDRITNGREGRAA
                     YLVSDEAFGDCEVYLEFMLAAGSNSGVFFNGKYEVQLFDSFGFGGVLMPGDNGAIYRL
                     WNNAGGWPPRVNASRPPGTWQSLHVWFRPPRFEGGKKTANARFLRVLLNETLVHEDVE
                     VAGPTGNHRDRTETPEGSIMLQGDHGPVAFRNIYIRPLDAP"
     gene            complement(1104..3335)
                     /locus_tag="VFQ79_13090"
     CDS             complement(1104..3335)
                     /locus_tag="VFQ79_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140649.1"
                     /translation="MNYGSQLFRVDHNISDRHRFFVRGNLGKRDQQFDTRFEEAQGAL
                     FHRYMRGIALDDVFTFSPALLLNVRYGYARFIEQNIPLQDKFDLASLGFAPSFIAEVG
                     QGPESSVKLPYLNISGYAPFGNQTLTNRADDAHTAGATLTHVVRSHMLRYGAEYRVYR
                     ENTYQLGQSSGQLDFGTSFSNGPLDSSAASPKGGALANFLLGIPTGGNVVSNDSYAYQ
                     NTELGLFVQDDWKITRRLTFSLGLRYEIPGPLTERFNRSVGGFDFSTASPIEAAARAN
                     YAARPIPEVPVESFRVLGGLTFPGVNGRPRHLMNRDRNNFAPRIGVAFSLDTRTVIRG
                     GYGVFYDQLGTSRLSFNQTGFSRTTSLVSSLDNGQTYIASIANPFPNGFQHADGTTAE
                     LATNLGQSVTFYNEELVNPYMQRWQWSVQRELFQNTVLEVAYVGNRGTKLRISRQTNP
                     IPREYLSTSPVRDQAAIDYLSAAVPNPFYPLLPGTGLSGTNTTRGQLLRPYPHFTGVS
                     RDTQQGYSWYHSMQTRFEKRFAAGYTVNVSWTWAKFMEATGYLNETDPMPERVISDQD
                     RPHRVVVTGVYELPFGRGRRWGSQWPGVLNGVLGGWQVANIFQYQSGPALGFGNAIFT
                     GDLAEIPLSGGERSRTRWFNTAAGFETDSRRQLGSNLRTLPSRFSGIRGDSMNNWDLS
                     LVKAFPLTERVVLHFRTEFINAFNHVMFNVPNTTPSSTAFGVVSGEVSKPRTVQFALR
                     LVW"
     gene            complement(3358..4554)
                     /locus_tag="VFQ79_13095"
     CDS             complement(3358..4554)
                     /locus_tag="VFQ79_13095"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0140650.1"
                     /translation="MLKTSAMLMLLLAAPLVAQDTRGQIVGRVQDASEAVIPGAEVRV
                     TNAGTNVTSTARTNRTGDFLLTYLVPGTYTLSVTYEGFREFVREGIPIRIGDRVTVDV
                     ILQPGSVTERVVVDAKAPLLEQASASMGQVIDTRRISELPLREGNPMLLANTVPGVLN
                     FAGSSSTDPSSVNGSSAFATSGTRSSNNDITLDGVANTARSTVAYVPPVDVVEEFRIQ
                     TASFDASQGFTPGSVVNVTLKSGTNQLHGSAYEFLQNDVLNANRFFSNMSGQEKPPLR
                     FNRWGINGTGPVYIPKLYDGRNRTFWMFGYEDSRSRQQRGTYSNTVPTAAERAGDFSA
                     LLQIGPQYQIYDPMTTRPAANNRFQRSPFPGNVLPASRIHPISQKLAQLWYSPNVPGT
                     IDGSLN"
     gene            4826..6541
                     /locus_tag="VFQ79_13100"
     CDS             4826..6541
                     /locus_tag="VFQ79_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0140651.1"
                     /translation="MEFRCFCWKTSRQLRRFPSEPGGRWSQSATFPVSLVRDWWKHAP
                     RPPPLAVHCGVGGIAVHQGPDHRAPTIRISVRLVQVDAVVTGSDGRPVTGLTAGDFEV
                     LQDGRTQDIVHCSYVPAHSGQGSESVSQQERLRPEQVRRTIALVADDLSLSFTSTGWV
                     REVLRRFVDEQMQPGDLVAILRTSGAMGALQQFTSDKRQLHAAISQLQWKMRRGFPVE
                     FPKELPEEFTPDGISEETETTPQSFGTIGALNYIVRGLKDVPGRKLIILFSDGLSLGG
                     RDTLARELRQRFSDMAGRASAVVYTIYAAGVQAPPSDPYMYDPLRTPMLSDSSPAMRN
                     LAAVSEQTGGRHFAYNDLPSEVSTILNQEAGYYFIGYRPDESTFRNPNRFHTLEVRVR
                     RAGLTVRSRRGFYGVAEENLPAVPDDPKRQLANVLISPFVSNTIPVRMEPLVAYSPKI
                     GQYVSCTLYLDARPLSFSQDAEGWMNGAIEVLLATFGVNGERVDVRAHTFHVRTRGKT
                     YRHVLENGLTYIITLPVKKPGPYQVRAAVRDVKSRKVGSAFEFVQIPSASRNRNANHK
                     RLPPG"
     gene            6585..6737
                     /locus_tag="VFQ79_13105"
     CDS             6585..6737
                     /locus_tag="VFQ79_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140652.1"
                     /translation="MAFLQSWILQRRLLHLMSPGEIRENREHILELTGRNPVHLCYPS
                     NEYRLD"
     gene            complement(6929..8257)
                     /locus_tag="VFQ79_13110"
     CDS             complement(6929..8257)
                     /locus_tag="VFQ79_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140653.1"
                     /translation="MNKISRRSMLYALAPAAVLGANDTVNVAVIGVGGRGRAHITMAG
                     KQTKCRIAAICDVNQAAQERAVAQVEKMQGHKPKVYADMRQLFDDREIDVVSMATPNH
                     WHALGAIWACQAGKDVYVEKPACHNVWEGQRMIDAARKYKRMVQVGSQSRTTAIKIRA
                     IELLEQGIIGKVYMAKGLCYKWRPSIGHTPDQPVPPGVNWDLFLGPAPMRAFSQNRFR
                     YNWHWFWDTGNGDLGNQGVHEVDICLWGLGETSMPNSVVSTGGKYVYQDDQETPNVQS
                     AVFDYGDRRMVFEVHGLHTGTDSDVAGGGGNIVGNVFYGTEGYLAVDPQGFRVFKGRG
                     CATTASAPSNCRREKVMEEKAVGPEGGYDSTAHFGAFVKAVRSRNANDLTAPVEVGVR
                     AAALCHLANISYRLGRKLAFDPAQGRFADADANRMLSREYRAPYVVPERV"
     gene            complement(8303..11473)
                     /locus_tag="VFQ79_13115"
     CDS             complement(8303..11473)
                     /locus_tag="VFQ79_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0140654.1"
                     /translation="MQKLAEICVARPVFAVMLVMAMVVIGGVSYGKLGVDRFPDVDIP
                     IVTVRTVLPGASPEEIESTVTRRIEDAVATVEGIDTLRSSSIESASILTITFNLERDI
                     DVAAQDVRDAVATVISLLPQDTKPPLIRKLDADAAPVMTLVLSGDKTPRELYEIADRQ
                     VQDAIESAGGVGQVQIVGGQRRAINVWVDADRLAAYRIPILDVRDAVARQNSDIPGGR
                     VDEGQRELVLRTLGRFPDPTLFNELVVATIGNTPIRIRDLGYAEDGHKEQRSSARYDA
                     REAVALEIQRQSGANTIQVIDNVKRRLESIRRLLPAGVDLDIVQDQSRYIEAAFHEVR
                     LHLILGSILASLVVLMFIRNWRATLIAAVAIPASIIATFGAMHWLGFTLNNITMLALV
                     LMVGVVIDDAIVVLENIFRFIEEKAIPPMQAAVMATKDIGLAVMATTLSLVVVFLPVS
                     FMSSISGRFLYSFGFTASVAILVSLVVSFSLTPMMSSRMLRRQDVTSHGGSRRGFYAH
                     VDRVYMRLLEAAMRRRAVVAGFALLVMVSGIPLYKLVRHEYIPTNVDESEFEMSVTAP
                     EGTSLTAMQDAMDRLSGELRSLPGIEHVLTIIGTGYLQQVNSGRAYVKLAPIEDRVFS
                     ISRLAKKTLEGHPWEAFRGVYSQRDVMQAVRERVKRYPDLRVSVRNSVSLNQGSAPVD
                     LDLALRGPDLMDLYRYSEHLRTVAAQTPGIVDVDTTLRLTKPELRVFIDRGRAADLGV
                     DASDIAGSLRIMVGGDDEVSRFRDEKAAEEYDVEVRLVGKDRDSATTVSKLWVPSAKS
                     GLTRLDNVVNMREAMSAFRIERLDRQRVVGIRANISPGYALADRLEEMYKAVDAMNLP
                     AAYTTMVVGRGREFERTLGEFLLAFLLSITFMYMVLASQFESLTHPVTILLSLPLAVP
                     FGFLSLWLFQDTLNLYSALGILVLFGVVKKNSILQVDHTNNLRRAGMERMQAILQANR
                     DRLRPILMTTLTLVAGMLPLALGAGPGAEERRSIAIIVIGGQSLSLLLTLIVTPVAYS
                     LFDDLGQWVSRRRLARVEVAESPAGD"
     gene            complement(11476..12558)
                     /locus_tag="VFQ79_13120"
     CDS             complement(11476..12558)
                     /locus_tag="VFQ79_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140655.1"
                     /translation="MQTARLENVPEVITATGELLAEESATISAKVAGRVQALRVDLGS
                     VVREGEVLAELEKEDYALQVRQSEALVEQMRARLGIASAAHDDVVPEQTATVRQTAAN
                     LSDARLLFDNTSKLFKEGVVSKLDYDRAQVNLQAAEARYQASLEEVAGMRAQLSERRA
                     QLALARQQLSDCTIRAPFSGAVTRRIASLGEYLAVNAPVVTLVRQHPLRLRLEVPERL
                     AARVRLGQRIDVRLEGSGMVSGGRVVRLSPAIQAENRSLTIEGEIPNQNGLLRPGSFV
                     EGSITVDERAKSILVPAEALTSFAGIERVFVVTGNVLDERVVKSGRRLSGERVEILSG
                     LEAGDRVVRKANDRMAKGQRVTAQGT"
     gene            complement(12682..14133)
                     /locus_tag="VFQ79_13125"
     CDS             complement(12682..14133)
                     /locus_tag="VFQ79_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HEU0140656.1"
                     /translation="MPTPKPLCFVLMPFGKKKDPTGLPDIDFNRIYRNAIEPAIKGAG
                     MIPVRADEEKTGGIIHKPMFERLLLCEYAVADLTTANANVFYELGIRHTARPCTTQPI
                     FAQHQPIPFDVNFLRALPYELGKDNAFGPRQAAALRRALTGRLSALRKIAIDRAPIDS
                     PVFQLIENWKPDLAHLRSDLFREQVAASEEIKQQMVQACSGPPGAARRALRKIEKKLG
                     LLDTHEVGIMVNLMLAYRSVEGWTELIELYERMPDTLKRQMMVREQLAFAFERRAEDQ
                     KAPRDARTYDRRRALEILEAVEAETGPNPETCGLIGRVYKALWAETAAAGDPAAEGHL
                     DTAIAAYVRGFEADPRDFYPGLNAATLLEVKGGPEAIGRRNQILPVVRFAAERKLRQA
                     HPDYWDYATMLEISVLDSDWAAARKYAGAALARVKETFQPKTTANNLRLIGAARKKRG
                     EAEAEIDSIVADFTKGSSRGPSRDPRPAGSMRK"
     gene            complement(14199..15602)
                     /locus_tag="VFQ79_13130"
     CDS             complement(14199..15602)
                     /locus_tag="VFQ79_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEU0140657.1"
                     /translation="MPGPSVFRAGASPLSGEAAAQGHTLYAYMVSLVAAASGLLFGFD
                     IAVINGAILLLRQQFALTEFQTEVAASSLLIGCVFGASLAGISSDRFGRRRILIVSAI
                     LFALSSAGAALPRDLTEFTAARFAGGLAIGVASVLAPLYIAEVSPARIRGRLVSLNQM
                     AIVTGILLAYLVNWLLSYHGPESWRWMFAAAILPSAAFFAGLLFVPESPRWLTENGAE
                     DKAFRVLMRVSGEAQARREIEDIKTAIAGESGSLRELFQPGLRRALLIAVTLAVLQQI
                     TGINTVLFYGSIIFKERVGGHTETAAIGANVIIGLVNFVATIVALWLIDRAGRKRLLM
                     WSSGVMAIAQLLLGIAFLLKPPPAALVLPAMLFCVAAFAVGLGPGVWVVLAEIFPTRI
                     RGRAMSIATISLWVACVALTMTFLSLAQAISISGAFWLYSGLCVVTFLFVLFVTPETK
                     GKTLEEIEEFWYRDRRS"
     gene            complement(15611..17014)
                     /locus_tag="VFQ79_13135"
     CDS             complement(15611..17014)
                     /locus_tag="VFQ79_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140658.1"
                     /translation="MYTDTRFTRQKFNLTLCGVPFVSFSAAAEEFSSMPWNAPATVAK
                     VYLASTRVHWPKTTLDVHAEVGEVESRLAEVARMHEQNVRFVGGDLLRTDEEVKPWLA
                     KVGDIDGVLIIPISQPIGPLRSLVDSLNVPALFFSRPYATHAWSSIAGLRKAGKKIDV
                     IATSSYGDLDPYMRLFRTVRHLRKSKVLVGVASPGARQEQADAYSRHFGTSFRFLNGA
                     EFHNAFNAVDKEKARGETDLFVRGALRVVEPSREEIFNGVRFYLGLKEMLLKEKANAV
                     TIDCFGTLAANTLPGYPCIAWSKFNDAGLYGVCEGDLASTMTQMLVTSYAGVPGFVSD
                     PVFDTSRNEVIHAHCVSATKLKGPDGPASPYIVRNHLETNEGAVLQVLMPSDETITVA
                     RFNGPTQMLISTAEVTGPVDSDRGCRSQIRTRVPGAGKWLQNFGAGLHRVIFYGDHVK
                     TVERMGRLMGFEVVHEA"
     gene            17164..19317
                     /locus_tag="VFQ79_13140"
     CDS             17164..19317
                     /locus_tag="VFQ79_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU0140659.1"
                     /translation="MRRLLLSGDEAVALAALESGVRLGTGYPGTPSTEILEAFSGLGG
                     RAQWSPNEKVALEVAIGAAFGGARALCTMKHVGLNVAADPLFTVAYTGVSGGLVIVSA
                     DDPGMASSQNEQDNRRYAEAAGVPALEPSDSQEAYEFTALAFEISERWEIPVLLRLTT
                     RVCHSKTVLHVPECAAGAVPLPRFTRDIHSRVMIPANARPAHRRLRAKLQAAREWNEA
                     SGPNRWMEGERALGIIASGAAYMHAREAAPAASFLKIGMTYPLPLDTIRKFATGVERC
                     VVIEEGDPFLADAIRAAGIQVEAKPEMYRFGELNVERVRRILANDVSPEPPLPRGTPP
                     QLCPGCPHRAVFSALRNLGCIVSGDIGCYSLGVLPPFEAMDTLVCMGASVGVGLGLRH
                     VLPPGQRRKVVSVIGDSTFVHSGITGLVEMIYNPPEDGHVLIILDNGTTAMTGMQEHP
                     GTGRTLQHTATGKMIFEDVIRALGIPRVHVIDPTIKPQEFENLIAACLDSLDLSVVIA
                     RRDCLLAASAIRQYEKCLEPVHEEKGRKPLADARGSVRAATARERTPNSLFQHPARTI
                     NIVIAGLGGQGVLKASDIAADAAFRAGFDVKKSELHGMSQRGGSVTSDVRFGESVLSP
                     MIPEGEADFLVAISPEQVEPNHHLLRPEGTLIHAGLIDAGALKNRKSVNVALLGKLSR
                     SLQLPDEAWMEAIRANLPEKLHEVNLNAFALGRKA"
     gene            19314..20651
                     /locus_tag="VFQ79_13145"
     CDS             19314..20651
                     /locus_tag="VFQ79_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEU0140660.1"
                     /translation="MSTLCEVIAARFQPASAPDYVPREHLRELQSARLRSVVERTYER
                     VQLFRSRMDRLGLHPQDIRDIEDLALLPFTTKADLRDTYPFGLFASPMDEVVRLHASS
                     GTTGKPIVVAYTQKDLAIWTNVMVRTFAAHGIHTGDIVQNAYGYGLFTGGLGAHCGAE
                     ALGATVIPISGGNTDRQIMVMKDFGVTVVCCTPSYFLHLLDRARELGVDVRSLPLRVG
                     VFGAEPWTESMRTRIEEQGGIKAYDIYGLSEIIGPGVAGECMCQAGLHVFEDHFYPEI
                     IDLETERPVKDGMEGELVLTTLTKSAMPMIRYRTRDITCILTEACPCGRTMRRIRKIS
                     RRSDDMFIIRGVNVFPSQIETALLDVEGTLPHYQIILTRAQGLDEIEVRVEVTPEVFS
                     DKIGALEGLHSRLIEALDRVLGLRVRIRLVEPQSIQRSEGKAKRVIDRRFEDQ"
     gene            20648..21079
                     /locus_tag="VFQ79_13150"
     CDS             20648..21079
                     /locus_tag="VFQ79_13150"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU0140661.1"
                     /translation="MKIDQISVFLENKPGHSIEPVRLLARAGIDIRTFSVADTREFGI
                     LRMIVSDPESATDLLRKVGYVVNLTEVLAVEVTDRPGGLAGVLEVFENTEINIEYLYA
                     FPVRRGDKAVLIFRFDNPDAAIDRLQRAGINVVETLEVYQH"
     gene            21084..22292
                     /locus_tag="VFQ79_13155"
     CDS             21084..22292
                     /locus_tag="VFQ79_13155"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0140662.1"
                     /translation="MPVSKTIAGQLKNASWIRRMFEEGNRLKAERGADRVFDFTLGNP
                     EVEPPEQVLEALSRAVSNRTPHRHGYMPNAGHIEARAAVARQLSVETGLPFTPDHILM
                     TVGASGACNVILKSILDPGDEVIGLMPCFSEYPFYVTNHAGRFVPVETDADFLPDIER
                     IAAAITPRTRAIILNSPNNPSGRIYPAGALEELEELLSQLDHPITVISDEPYKRLIFD
                     GRKQTPAASRISQTAICNSWSKSQAIPGQRIGYLALSPRLAGLEEIRNACTFANRILG
                     FINAPAIWQWVEAEAAEAVVDVLAYQEKRDRLCNALTAIGYDVQKPEGTFYVFPKTPV
                     SDDVEFVRLLLNEGVLGVPGSGFGRSGYFRLSLTVPMETITNSLPAFERVFHQCKEAR
                     PSADSFQPSR"
     gene            22244..23584
                     /locus_tag="VFQ79_13160"
     CDS             22244..23584
                     /locus_tag="VFQ79_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEU0140663.1"
                     /translation="MQRSTAFRRLLSAIALGSCVLPGQAQQNIEPRLFEYGFEQRVRT
                     ENWNNILDYDAARADERRQIRYRSKLWMKSPVTSDIDVFVALNQETNQILVPDVPQRF
                     DEVMVENAYVDFRKLFVKGLSLRVGRQNLMRGEGFLLLDGSPGDGSRSIYFNAAVLGY
                     SWSKSKLEAMAIHNPSNDRYLPRLHASTTRQLAERNEQALALYYTNDQVSNTSIDAYY
                     FLKRETRDCRAATQHQYQADRHVHTAGGRLTRRMQGGWSAASEFAAQWGREEPGERIA
                     AWGGYGYLKKTFARPGDPYVLTGYWGMSGDDPMTSTRYEGWDPLFARWPKWSDLYIYS
                     YIREQGVSYWTNTWLWQLEAGYSPWKRIKTSFTCYDMNAFHPFNADPLNYSDGKHRGR
                     QYQARAELSVNDHWRGHILYERHLPGSFHAGHSSGWFFRMEVIYSFQGKIPLQH"
     gene            23643..24191
                     /locus_tag="VFQ79_13165"
     CDS             23643..24191
                     /locus_tag="VFQ79_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEU0140664.1"
                     /translation="MCNRIFALSLLVFALPALPQQTRTEVTKATTPKDDSKPNSDKVP
                     EVYAISGQFERVVVLRYKFGTDLLAGLEKMVKEQNIRNAVILAGAGSVRNYHVHSVSN
                     RTFPSKNIYVKDPTASADIVSMNGYIIDGRVHAHMTMTNSEGAFGGHLEAGTNVFTFA
                     IITLGVFNDSVDLSRVDDKTYR"
     gene            24336..25373
                     /locus_tag="VFQ79_13170"
     CDS             24336..25373
                     /locus_tag="VFQ79_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0140665.1"
                     /translation="MSAPGSAAMKSFGTALRTSVGSLASRSLGKAFRNYEPAIDLFPA
                     DLRARVAGRVLRRAQQALDLIARILLDPGDCVWLEDPGYPGARSVFHATGAHIIPVPV
                     DRDGLDVAAARALAASTRVVYTTPAHHFPLGVTMPLERRLALLNWAINAGAWIVEDEY
                     DAEYRYFGRPVAVLQGLDRSGSVIYVGTFTKMLFNSLRLGFLVLPERLAGAFEAMRSF
                     VDRHPPTLDQAILAEFILEGRFGHNVRRMRQTYAERMAVLKQAAQRHLAGLLDVENAA
                     AGMHTIAWIKTGAPDTVLAARARDRGLELSALSSFSVRHYHPPALVLGFAGSSPSELR
                     RGVRVLASALT"
     gene            25606..27018
                     /locus_tag="VFQ79_13175"
     CDS             25606..27018
                     /locus_tag="VFQ79_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU0140666.1"
                     /translation="MATTVSKYPRAGLWQSVKEAIRGTEYDYTELPITHSVFLLAIPM
                     VLEMAMESLFAVVDVFWVSRLGANAIATVGLTESMLTLVFAVAMGVSLSTTAMVARRI
                     GEHDQQGARVAAAQAILLGAGLAVAMGVPGFWLAPRLLEWMGGPPELVETGQHYTALV
                     LGGSITVMLLFLNNAIFRGAGDAAVAMRVLWFSNLINMALDPFFIFGWGPFPEMGVTG
                     AAVATVIGRSSGVLYQFWILASRQSRIQLRWTDMRLNWPVMLSLIRVSTTGVLQFAVA
                     HTSWILLVRIISTFGSAAVAGYTVGIRIFAFAILPSWGLSGAAATMVGQNLGAQKPGR
                     AERAVWLTGFYNMIYLGAVAIVFIVLPAPLVRLFTSDPEVIAAAVNCLRIVAIGNIAY
                     AYGMAMVQAFNGAGDTVTPTIINIFGFWLCEIPLAWTLALPGGMGVNGVFASIPISEG
                     LITVISLIVFRRGKWKQRKI"
     gene            complement(27055..29028)
                     /gene="shc"
                     /locus_tag="VFQ79_13180"
     CDS             complement(27055..29028)
                     /gene="shc"
                     /locus_tag="VFQ79_13180"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEU0140667.1"
                     /translation="MREEFRYIAPAPPRDTEAARTIAQAELEQLAAGIQASASAAANA
                     LLKIQDPQGYWCGDLTADSTLESDYILLQLWLHPPEPDQPWRPPAWNRIRKAVRFILE
                     RQLPDGGWSIYEGGPSEVNATARAYTAMKIAGEDPESPAMVRARSRVLSLGGLQAGNS
                     YTKMNLSLFGLYPKRYVPSVPPEILLVPGNLLYEMSSWTRTILVPLSIVQSIGGHHPV
                     PHSLTVEELFVPGKRLTLPRRDRASTVFNHADRLVKMWERRGIKDVRKTAVREAERWV
                     LERLHHADGLGAIYPAMMYTIMAMDALGYERDHPDLIEAIAQFDRLLLETEDRLQFQP
                     CFSPVWDTAIAAFAVGEMPGGPVDAAAMTRAADWLLDKEVRRKGDWSIKRPDLRPSGW
                     AFEFANEYYPDIDDTAMVLLALQHARASDPERQERVERRAIEWLLGMQSSDGGWAAFD
                     VDNNWEFLNKVPFADHNAMLDPTCPDITGRVLEALCRRGLKTGHPAVRNAVRYLIANQ
                     KPDGSWYGRWGVNYIYGTFLAVRGLRASGDLSARPSMQRAAAWLQSIQNEDGGWGESC
                     ASYARHYYVPASSTPSQTAWALLGLSAANAGQSSAVHRGLRYLIDTQRPDGTWDEKLT
                     TGTGFPNVFYLTYHLYRQYFPLLALATCHIGDN"
     gene            complement(29025..29609)
                     /locus_tag="VFQ79_13185"
     CDS             complement(29025..29609)
                     /locus_tag="VFQ79_13185"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU0140668.1"
                     /translation="MKKRCPWAKSDLDILYHDSEWGVPQHDDRVLFEFLILEGAQAGL
                     SWATILKKRENYRSAFDGFDPAVIARYEERKIGELLANSGIVRNRLKISAAVRNARSF
                     LDVIDEFGTFDRYIWQFVNGTPVVNAWRTVDEVPARTPVSDAMSKDLAKREFRFVGST
                     ICYAFMQAVGMVNDHLVTCHRHREISEKTLSFRS"
     gene            29683..30996
                     /locus_tag="VFQ79_13190"
     CDS             29683..30996
                     /locus_tag="VFQ79_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140669.1"
                     /translation="MIGPKLFTACFLFFLLQQSANAFDAEARIRAEAAFREGVAAEST
                     GDNARAMRAFTQALEADPDYGPAWLRRGRLQLKAADYKQAIADLNLAVAAGLENADLY
                     ETRGAAYSQLEHWKYAIADYTRCVEADAKRTACWTGRGFAYGQIEQFGSAARDLTRAL
                     ELGDSDPRTYALRGAAYFKTGEMEKAIADLTKALESQPNDPLLYLVRAAAYAETGDQQ
                     QALRDREQAVRLDSRNAEAYVARGASYHALGRHEEGFADRTRALGLNPQFAEAYLGRG
                     NAHLSLGQYAEAVEDLQKLVELKPDDAEARALLQRALDAQQKATEAPKPIPQETAGGG
                     KETAVSKAVPASGAALARKHYETGRKLLDQAKFSAAVDELSKAIALNAKNAFAYNARG
                     YAYLRLKQYKQAIADFDAALRLNPRYRNASRNREAARRAAQTPQQ"
     gene            31080..31448
                     /locus_tag="VFQ79_13195"
     CDS             31080..31448
                     /locus_tag="VFQ79_13195"
                     /inference="COORDINATES: protein motif:HMM:NF022970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEU0140670.1"
                     /translation="MKKPRKDPVRENRIHEEAIVDAYGPEEQAMSWYCYLEGKLSFPF
                     PATCTASKITSPLKKGETVDVLRMAPDEACTTDMLVLVRRQGRKLAVPLAQLKPVDPD
                     ESTDEAAGDWHYWVAQGYRF"
BASE COUNT         6479 a         9424 c         9471 g         6198 t
ORIGIN      
        1 atctggggcg gcgagttcgg ccgtaccgtg tactgccagg gtaagctcac cacaaccaat
       61 tacgggcgcg atcaccaccc gcgttgcttc gcgatgtgga tggccggggg cggcattaag
      121 gcgggtgttt cgctcggcga aacggacgac tactgctaca acgtcgtcga ggacccggta
      181 cacgtacacg acatccaggc gacggcgctt cattgcctgg gcatcgatca caaacgtctt
      241 acatacaagt ttcagggccg gcacttccgg ctcaccgacg ttcacggcga actggtcaag
      301 aaaatcctgg cgtagcttcc gccagctcta ctaaggcgcg tccagcggcc ggatgtagat
      361 gttgcggaac gctaccggcc catgatcgcc ttgcagcatg atagagcctt ccggggtttc
      421 tgtgcggtcc ctgtggttgc cggtggggcc ggccacctcc acgtcttcat gcaccagcgt
      481 ttcattcagc aggactcgaa gaaaacgcgc attcgcggtc ttcttgccgc cttcaaaacg
      541 cggcgggcga aaccagacat gcaacgattg ccacgtaccc ggcgggcggc tggcgttgac
      601 gcgcggtggc cagcccccgg cattattcca gaggcggtag atggctccat tatcgccggg
      661 catcaagacc ccgccgaatc caaaactgtc gaatagctgc acttcgtact tcccgttgaa
      721 gaagacaccg gaattagacc cggcggccag catgaactcg agatatactt cacaatcgcc
      781 gaaagcctca tcgctcacca gatacgcggc gcggccctct cggccattgg taatgcggtc
      841 gccgggaccc ggggtgaacg taagccgctt cgggtcgaag atgcggcgcc agatcacact
      901 gcgggtggtg acccaaccgt gcggatcccg cccggcgcca tgccagccgt ttaggtcctg
      961 gccattcagc agaggccgcc accctggttg cgaaagaaaa ggagggtcgc catagctttc
     1021 ggtggtatag aggtccggga cctgggcgaa cgccggagcg agcgcccaaa caaaaaccgc
     1081 tagaaacgcg cgcaacatgg ggatcaccac acaagacgca gtgcgaattg taccgttcgg
     1141 ggctttgaca cctcccccga cacaaccccg aaggccgtgc tgctcggagt cgtattgggc
     1201 acgttgaaca tgacgtgatt gaacgcgttg ataaactccg tgcggaagtg tagtaccacg
     1261 cgttcagtaa gaggaaaggc cttcaccagc gataaatccc agttattcat gctgtcgccc
     1321 cggatgccgg aaaaacgaga gggtagcgtg cggagattag aacctaattg ccggcggctg
     1381 tcggtctcga agccggccgc ggtgttgaac cagcgcgtgc ggctgcgttc tccgccggag
     1441 agcggtatct cagcaagatc gcctgtgaag atggcattgc cgaagcccag tgccgggccg
     1501 ctttggtact ggaatatatt ggccacctgc cagccgccga gcactccatt cagcactccg
     1561 ggccattggg aaccccaacg gcgcccacgg ccgaagggca gctcgtacac accagtcact
     1621 acgacgcggt gcggcctgtc ctgatcggaa atgactctct ccggcatagg gtccgtctcg
     1681 ttcaagtatc cggtggcctc cataaacttc gcccatgtcc aggacacatt cacggtgtag
     1741 cccgcggcaa agcgtttctc gaaccgcgtc tgcatcgagt gataccagga gtaaccttgc
     1801 tgggtatcgc ggctcacgcc ggtgaagtgg gggtagggcc gcagcagttg gccgcgtgtc
     1861 gtattggttc cggatagccc ggtgcccggg agaagggggt aaaacggatt cggaaccgcg
     1921 gcactaaggt aatcgatggc cgcctggtcc cgcacggggg acgtactgag atactcacgg
     1981 ggtatggggt tggtttggcg gctgatacgt agcttggtcc cgcggtttcc aacataggcg
     2041 acttcgagca ctgtgttctg gaacaactcg cgctgtacgc tccattgcca gcgctgcata
     2101 tacggattta caagttcctc gttgtagaag gtcaccgatt ggcccaggtt cgtcgccagt
     2161 tccgccgtag tgccgtcggc gtgttgaaaa ccgttaggaa agggattggc gatgctggca
     2221 atgtaggtct ggccgttatc cagactggag accagagatg tggtccggct gaatcccgtc
     2281 tggttgaagc taaggcgtga ggttcccaac tgatcgtaga agacgccgta tccgccgcga
     2341 atgacggtgc gggtatccag cgaaaaggcg acgccgatac ggggagcgaa gttgttgcgg
     2401 tcgcgattca tcaaatgccg gggccgcccg ttgacgccgg gaaaggtcaa gccgcccaga
     2461 acgcgaaaac tttctacggg cacttccggt atcggcctgg ccgcgtagtt ggcacgcgca
     2521 gcggcttcaa tcggactggc cgtggaaaag tcgaatcccc ccacgctgcg gttgaagcgc
     2581 tcggtcagcg gtcctggtat ctcatagcgc aaccccaggg aaaacgtaag gcggcgggtg
     2641 atcttccaat catcttgcac aaaaagtccc agctcggtgt tctgatatgc gtaggaatcg
     2701 ttgctgacga cgttcccgcc cgtgggaatg ccgaggagga agttggccaa agcgccgcct
     2761 ttgggtgacg ccgcggagct atccagcggg ccattactga agctggttcc gaaatcaagt
     2821 tggccggagg attgcccaag ctggtacgtg ttctcgcgat agacacgata ctccgctcca
     2881 tagcgcaaca tgtggctacg cactacatgc gttaaggtgg ctcctgccgt atgggcgtcg
     2941 tcggcacggt tggtaagcgt ctggtttccg aacggtgcgt aaccggaaat attcaggtag
     3001 ggcaacttga cggagctttc gggcccttgt ccgacttcgg ctatgaaact tggcgcgaat
     3061 cccagagagg ccaggtcgaa cttgtcctgt aaggggatgt tctgctcgat gaaccgggcg
     3121 tatccatagc gcacattgag aagcagcgca gggctgaagg tgaagacatc gtcaagggca
     3181 ataccccgca tatacctgtg aaataaagct ccctgggctt cctcgaaacg cgtatcgaac
     3241 tgctggtcgc gcttccccag attgccgcgg acgaagaacc ggtggcggtc gctgatgttg
     3301 tgatccacac gaaatagctg gctgccataa ttcacgcttt cgggccccgg atcggtgtta
     3361 gttgagggag ccgtcgattg tgccgggcac attggggctg taccagagtt gcgcaagctt
     3421 ctgcgagatc ggatgaatac ggctggcggg cagcacgttt ccgggaaagg ggctgcgttg
     3481 gaaccggttg tttgccgccg gcctggtggt catcgggtcg taaatctggt actgcggacc
     3541 gatctgaagc aacgcggaga agtcccctgc cctttcggca gcggtgggca ccgtgttgga
     3601 atacgtgccg cgctgctggc ggctgcgcga gtcttcgtaa ccgaacatcc aaaaggtgcg
     3661 atttctgccg tcgtacagtt tagggatgta aacggggccg gtgccattta tgccccatcg
     3721 gttgaagcgg agcggcggct tctcctgccc cgacatattg gagaagaacc gattggcgtt
     3781 gagcacatcg ttttgcagaa actcgtacgc tgagccatgc aactggttgg tcccggattt
     3841 caaagtcaca ttgaccacgc ttccaggagt gaagccctga ctggcatcaa acgaagccgt
     3901 ctggattcga aactcttcca ccacgtccac cggcggcacg taggcaacgg tgctgcgcgc
     3961 ggtgttggcc acaccgtcga gggtaatgtc gttgttggag gagcgagtgc cgctggtggc
     4021 gaacgccgat gagccattta ccgaggaagg atcggtggag gacgagccgg caaaattcag
     4081 tacgccggga acggtgttgg ccagcagcat cgggttgccc tcccgaaggg gcaactctga
     4141 aatgcgccgg gtgtcgatca cctgtcccat ggaagcgctg gcctgctcca gcaaaggagc
     4201 tttggcatct acaactacac gctcggtgac gctgccgggc tggagtatga catcgacggt
     4261 tacgcggtcg ccgatacgga taggaattcc ttcgcgcaca aattcgcgaa acccttcgta
     4321 ggttaccgat aacgtgtatg tgccggggac cagataggtt agcagaaaat cgccggtccg
     4381 attagtccgg gcggtgctgg tcacgttggt gccggcattc gtgacgcgca cctccgctcc
     4441 cggaatcact gcctcggaag cgtcctggac ccgaccgacg atttggccgc gcgtgtcttg
     4501 agccaccaat ggcgcggcga gaagcagcat gagcattgcg gatgtcttca gcaaattaga
     4561 atctccccag agacgttttt aagcggtccg gcgcccacat cgggtgcgtg gttccgcgtt
     4621 ggagcgttcc aaacatgata ttcacagacc cggaaagtgt aaagcgtttc gtcctaattt
     4681 tccgattttc cccattcttt tgttatttcc tcaaggcaac tatgcgccgg ggaagcctcc
     4741 agccggtgga ggaaatggcg gtcaggaact attgcaggcg tatagaacaa ttccggattc
     4801 ctgctggatc gcgcttgtat gctcgatgga attccgatgt ttctgctgga agaccagccg
     4861 ccagttgcgg cgcttcccct ccgaaccggg tggaagatgg tctcaaagcg caacgtttcc
     4921 tgtttctctc gtgagagact ggtggaagca tgcgcccagg ccgccgcctc ttgccgtgca
     4981 ttgtggtgtt ggcgggattg cagtccacca aggcccagac catcgagccc ccacgattcg
     5041 tatctccgtc agactcgttc aggtggacgc tgttgtcact ggttcggacg gacgtcctgt
     5101 gaccggtctg acagccggag attttgaagt tcttcaggac gggcgcacgc aagacatcgt
     5161 tcactgttct tacgtgccgg cccattccgg acaaggcagc gaaagcgttt cacaacagga
     5221 gcgtctgaga ccggagcagg tgcggcgcac catcgccctg gtagcggacg acctgagcct
     5281 gagtttcacc agtacaggct gggttcgcga agtactgcgg cggttcgtgg atgagcagat
     5341 gcagccgggg gacctggtgg ccattctccg cacgtcggga gccatgggag cgctgcagca
     5401 gttcacgtcc gataaacggc agttgcacgc cgccatcagc cagcttcagt ggaagatgag
     5461 gcgcggcttt ccggtcgagt ttcccaagga actgccggag gaattcacgc cagacgggat
     5521 ttccgaagaa accgaaacca cgccgcagag tttcggtacg atcggcgctt tgaactatat
     5581 cgtgcgaggc ctcaaagatg tacccggacg caagctgatt atcctcttct ccgacgggtt
     5641 gtcgctgggc gggcgggaca cattagcccg cgagttacgg caaaggttca gcgacatggc
     5701 gggccgggct tcagcggtgg tttataccat ctatgccgct ggcgtgcagg cgccgccgag
     5761 cgacccctac atgtacgatc ctctccgtac tcccatgttg agcgactctt cgcccgcgat
     5821 gaggaacctg gcggccgttt ccgaacagac gggcgggcgg catttcgctt acaacgacct
     5881 tccctctgaa gtctcgacaa ttctcaacca ggaggcgggt tattacttca tcggttatcg
     5941 tcccgatgaa tccaccttcc ggaatccgaa tcgcttccac accctggagg tgcgggtgcg
     6001 gcgcgcggga cttacagtcc gttcgcgacg cggcttctac ggcgtcgctg aagaaaatct
     6061 gccggccgtg ccggatgatc ccaaacggca gcttgccaat gtcctgattt ccccgttcgt
     6121 ttcaaacaca atcccggtgc gcatggaacc gctggtcgcc tactctccga agatcggcca
     6181 gtacgtttcc tgcacgttgt accttgacgc gagacccctg tccttctccc aggatgcgga
     6241 gggctggatg aatggagcga tcgaagttct gctggccact ttcggcgtca acggagaaag
     6301 agtggacgtc cgcgcgcata cgtttcacgt tcgcacacgc ggaaaaacct atcggcatgt
     6361 tctcgaaaat ggactgacat atataattac cttgccggtc aaaaaaccgg gaccatacca
     6421 ggttcgggca gcggtgagag atgtgaaatc acgcaaagta ggctcagcct tcgagttcgt
     6481 ccagattcct tccgcatctc gaaatcgtaa cgccaatcac aaacggttac cgcccggata
     6541 aatactctgg aaagcccgtg gccgcgttct gaagggccgt aattttggca tttcttcaaa
     6601 gttggatctt acaacgccgg ctgctccacc tcatgtcgcc cggtgagatt cgagagaacc
     6661 gcgaacacat tctcgagctt accggccgga acccggtgca cctttgttat ccgagcaacg
     6721 aatacaggct cgattgactt ccgtggctta cccaactgtc ggtctcttcg gctacgacct
     6781 gcaggcaggt cttgcattcc cggcgttcaa acacgctgtt gctaccccgg ctcgtcgatc
     6841 actgtggatt gagcaacatc gaatttgaaa gctggctttg cggctgcgcc gaattcctcc
     6901 cgaaccgcgg ataggccatg cgggcatttc acacccgctc cggcaccacg tagggtgcgc
     6961 ggtactcgcg gctgagcatg cggttggcgt ccgcgtcggc gaacctgccc tgcgccgggt
     7021 cgaatgccag cttgcggccc agccggtagc tgatattggc taagtggcac aacgccgcgg
     7081 cccgcactcc tacctccacc ggcgcagtga ggtcgttggc gttccggctg cgaaccgctt
     7141 ttacaaacgc cccgaagtgt gccgtgctgt cgtagccgcc ctccggcccc acggccttct
     7201 cttccatcac cttctcgcgc cggcagttcg agggagcaga ggctgtcgta gcgcagccac
     7261 gccccttgaa aactcgaaag ccctgcggat cgacagccag atatccttcg gtaccgtaaa
     7321 acacattgcc cacaatgttg cccccgcccc cggccacatc gctgtccgtg ccggtgtgca
     7381 gaccatgtac ctcgaaaacc atccgccggt cgccgtagtc gaagaccgcc gattgcacgt
     7441 tgggtgtctc ctggtcgtcc tggtagacgt acttgccccc ggtggagact accgaattgg
     7501 gcatactggt ttcgccaagt ccccacagac agatatccac ttcatgcact ccctggttcc
     7561 ccaggtcgcc gttgcccgta tcccagaacc agtgccagtt gtatctgaag cgattctggc
     7621 tgaaggcgcg catcggcgcg ggccccaaaa acagatccca attgaccccg ggcggcaccg
     7681 gctggtccgg cgtgtgcccg atggagggcc gccacttgta gcacaatccc ttcgccatat
     7741 agaccttgcc gatgatgccc tgttcgagca gttcaatggc acggatcttg atcgccgtgg
     7801 tgcggctttg cgaaccaacc tggaccatcc gcttgtattt gcgcgccgca tcgatcatcc
     7861 gttggccttc ccacacgttg tggcacgcag gcttttccac gtatacgtcc ttgcccgcct
     7921 ggcaggccca aatcgcaccc agcgcgtgcc agtggttagg cgtggccatg gagacaacgt
     7981 cgatctcgcg gtcgtcgaag agctggcgca tgtcggcgta gactttgggc ttgtggcctt
     8041 gcatcttttc cacctgggca acggcgcgct cctgcgccgc ctggttcaca tcacagattg
     8101 cggcgatgcg gcacttggtc tgtttgcctg ccatcgtgat gtgggcacgg ccgcggcctc
     8161 ccacgccgat caccgccacg ttgacggtat cgttggcgcc cagcaccgcg gcgggtgcca
     8221 gggcgtagag catcgatcgc ctgctaatct tattcattgt caaaatctcc agtacttaaa
     8281 gcgttctttc ctctcatcgg gactagtcgc cggcgggcga ctccgctacc tcaacacgcg
     8341 ccagccgccg ccttgatacc cactgcccta aatcgtcgaa cagcgagtag gctacaggcg
     8401 ttacgatcaa cgtaagcaac aacgaaagac tctggccgcc gatgacaatg atcgcaatcg
     8461 aacggcgctc ctccgcaccg ggaccggcgc cgagcgccag aggcagcatg cctgcaacca
     8521 gcgtcagcgt cgtcatcaga ataggccgca ggcgatcccg gttcgcctgt aggatcgctt
     8581 gcatccgctc cataccggcc cgacgcaagt tgttcgtgtg atccacctgt aaaatcgagt
     8641 tctttttcac cactccgaag agcaccagaa tacccaatgc cgaataaaga ttcagcgtgt
     8701 cctggaacag ccatagcgac aggaaaccga acggaaccgc gagcggcagg ctgagcagaa
     8761 tcgtgaccgg gtgtgtcagg ctttcaaact gcgaagccag aaccatgtac atgaatgtga
     8821 tggaaagcag aaaggcgagc agaaactcgc ccagcgtccg ctcgaactcc cgtccgcggc
     8881 ccaccaccat cgtggtatac gctgccggaa gattcatcgc gtcgacggct ttgtacattt
     8941 cttccagcct gtcggccaac gcgtaacccg ggctgatgtt ggcgcgtatg ccgaccacgc
     9001 gctgccggtc gaggcgctcg atgcggaatg ccgacatcgc ttcgcgcatg tttacgacat
     9061 tgtcgagccg tgtcaggccg ctcttcgccg aaggcaccca cagcttcgaa acggtggtgg
     9121 cgctatcacg gtccttcccc accaggcgca cttccacgtc gtattcctca gccgcttttt
     9181 cgtcccggaa acgtgaaacc tcatcatcgc cgcccaccat aatgcgcagc gatccggcaa
     9241 tatcggaagc gtccacaccg aggtccgccg cacggccgcg gtcgatgaag acgcggagtt
     9301 cgggtttggt gagccgcaga gtggtatcca cgtccacgat gcccggagtt tgtgccgcca
     9361 ccgtccgcag atgctcgctg tagcggtaca agtccatcag gtccgggccc cgcagcgcca
     9421 ggtccagatc cacgggcgcg gacccctggt tcagagaaac cgaattgcgc accgatacac
     9481 gaagatccgg atagcgcttg acgcgctcgc gaaccgcctg catcacatcg cgttgcgagt
     9541 atacgccacg aaacgcctcc catggatgcc cttccagcgt cttctttgcg agccgcgaaa
     9601 tactgaagac acgatcttcg atgggcgcca gcttcacgta cgcgcgcccg gaattcacct
     9661 gctggagata gcctgtgccg atgatggtaa gcacgtgctc gatgccgggc aggcttctaa
     9721 gctcccccga aaggcggtcc atcgcgtcct gcatcgccgt taaactggtc ccttccgggg
     9781 cggtgacgga catctcgaat tcgctctcat ccacattcgt ggggatgtat tcatgccgca
     9841 cgagtttgta caggggaatg ccggaaacca tcaccagcag agcgaaaccg gcgacaacgg
     9901 cgcgacggcg catcgccgct tcgagcagcc gcatgtagac ccggtccacg tgcgcgtaaa
     9961 atcctcgccg cgaaccgcca tggcttgtca catcctggcg ccggagcatg cgtgagctca
    10021 tcatcggcgt caggctgaag ctgacaacga gagagacgag aatggcgacc gaggccgtga
    10081 acccgaagct gtacaggaac cggccggaga tgctggacat gaacgacacc ggtagaaaca
    10141 ccaccaccag gctaagagtt gtcgccatca ccgccaagcc gatgtccttc gtcgccatca
    10201 ccgcggcctg catcggcgga atcgcctttt cctcgataaa gcggaaaatg ttttcgagca
    10261 cgacaatcgc atcgtcgatc accacaccca ccatcaggac cagcgccagc atggtgatgt
    10321 tgttgagcgt gaatccaagc cagtgcatgg cgccgaacgt ggcgataatg gacgcgggaa
    10381 tcgccacggc ggcaatcagc gtcgcccgcc agttccggat aaacatcaat acaaccagcg
    10441 aggcaagaat gctgcccaga atcaggtgca gccgtacttc atggaaagcc gcctcgatgt
    10501 agcgcgattg gtcctgcaca atgtccaggt cgacgcccgc gggcaacagg cgccggatgc
    10561 tttcaaggcg ccgcttgaca ttgtcgataa cctgaatggt gttggcgccg gactgccgct
    10621 ggatttcgag tgccaccgct tccctggcgt cgtagcgcgc cgaactgcgc tgctccttgt
    10681 ggccgtcttc cgcatatccc aggtcacgga tccgaatcgg ggtgttgcca atcgtcgcca
    10741 ccaccaactc gttaaacaat gtgggatccg gaaaacgccc cagcgttcgc agcaccagtt
    10801 cgcgctgtcc ttcatccaca cgcccgccgg gaatatccga attctgccgc gccaccgcgt
    10861 cccgcacgtc gagaatcgga atgcggtagg ccgccagcct gtccgcatct acccaaacat
    10921 tgatggcgcg ccgctgcccg ccgacgatct gtacctgccc gacaccgccg gcggattcga
    10981 tagcatcctg cacctggcgg tctgcgattt cgtacaactc gcgaggggtt ttatcgccgc
    11041 tgagcaccag cgtcatcacg ggcgcggcgt cggcgtccag cttgcgaatc agcggcggct
    11101 tggtgtcctg tggcagcagg ctgatgaccg ttgcaaccgc gtcccgcacg tcctgcgccg
    11161 ccacatcgat atcgcgttcg aggttgaaag tgatggttaa aatcgaggcg ctctcaatgg
    11221 aagaggagcg cagcgtgtcg atgccttcca ccgtggccac ggcgtcttcg atacgccgcg
    11281 tgacggtgct ttcaatctct tccggcgatg cgccaggcag cacggttcga acggttacga
    11341 tcggaatatc gacatcgggg aagcgatcga caccgagctt gccgtaagac acaccgccga
    11401 ttacaaccat cgccatgacg agcatcacgg cgaataccgg acgtgcgacg cagatttccg
    11461 cgagcttctg catgctcagg tgccttgtgc ggtcacccgc tggcccttcg ccatgcggtc
    11521 gtttgctttt cggactacgc ggtcacccgc ctcgagaccg gataggattt cgacgcgttc
    11581 gccagacagg cgccgaccgg attttacaac gcgctcatcc agcacgttac ccgttaccac
    11641 aaacacacgc tcgatgccgg cgaaactggt gagcgcctct gccggtacga gaatgctctt
    11701 ggccctttca tccaccgtaa tggaaccctc tacaaacgat ccgggccgga gcaatccatt
    11761 ctggttgggg atctcgcctt cgatagtcaa tgaacgattt tccgcctgga tggccgggct
    11821 caaacgtaca acgcggcctc cgctcaccat gcctgaacct tccaaacgga cgtcgatccg
    11881 ttggccgagc cgtacgcgtg cagcgagccg ttccggcacc tccagtcgca gacggagcgg
    11941 atgctgccgg accagcgtta ccaccggtgc gttgaccgcc agatactcac ccaacgaggc
    12001 aatacgccgt gtcaccgcgc cggaaaaggg ggcacggatt gtgcaatcgc tgagttgctg
    12061 ccgcgccagc gccaattggg cgcgccgctc actcaactgc gcccgcatgc cggccacttc
    12121 ttcgagggaa gcctggtagc gggcttccgc agcctgcaag ttcacctggg cgcgatcgta
    12181 gtcgagcttc gaaacaacgc cttccttgaa cagcttcgac gtattgtcaa acaacagccg
    12241 cgcatcgctg aggttcgccg ctgtctgccg gaccgtggcg gtctgttccg gaacgacgtc
    12301 atcatgcgcc gcggaagcga tgcccagccg cgctctcatc tgctccacca gcgcctcgga
    12361 ttgccgtacc tgtagcgcgt aatcttcctt ttccaactcg gccaggactt ccccttcccg
    12421 aactacgctg cccaggtcca cgcgcaaggc ttgaacgcgt ccggcaacct tggcactgat
    12481 cgtagccgac tcttcggcga gcagttcccc ggtcgcggtg atcacctcgg ggacgttttc
    12541 gagcctcgcg gtttgcacct cgactgcgac cggacgaggg ggctcgttag ccgatacagg
    12601 cccgcccgag tatggaccgc tacagccaac agcccacagc gcacaacatg agacaaacag
    12661 ccggatcatg gtaggtttgc gttattttct catagatccg gcggggcgtg gatcacggga
    12721 cgggccgcga gaagacccct tagtaaaatc cgccacgatg gagtcgattt cggcctccgc
    12781 ctccccgcgc tttttcctgg cggctccgat gaggcgcaga ttgttcgccg tcgtcttggg
    12841 ctgaaacgtc tccttcacgc gagcgagggc agcgcccgcg tatttgcgtg ccgcggccca
    12901 atccgagtcg agcaccgaga tctccagcat tgtcgcgtag tcccaatagt ccgggtgcgc
    12961 ctgccggagc ttacgctccg cggcgaaacg tacaaccggc agaatctgat tccgccggcc
    13021 gattgcttcc gggccgccct tcacttccag cagcgttgcc gcgttcaggc cgggatagaa
    13081 gtctctcggg tccgcttcga atccgcgcac atacgccgcg atcgctgtat cgagatgccc
    13141 ttcggcggcc gggtcgcctg cggcggcggt ttccgcccac agcgccttgt acactctgcc
    13201 gatgagaccg caggtttcgg ggttcggacc ggtctccgct tccaccgcct ccaggatctc
    13261 cagcgcgcgc cggcggtcgt aggtccgcgc gtctctggga gctttctggt cctcagcgcg
    13321 cctttcgaac gcgaatgcaa gctgctcccg caccatcatc tgccgcttga gggtgtccgg
    13381 cattcgttcg tacagctcga tcaactccgt ccagccttca accgaccggt aggcgagcat
    13441 caggttcacc atgatgccga cttcatgagt atccagcagc ccgagtttct tttcgatttt
    13501 acggagcgct ctgcgggcag caccaggcgg cccggaacag gcctggacca tttgctgctt
    13561 gatctcctcg ctcgccgcca cctgttcgcg aaacaggtcg ctacgcaggt gggcaaggtc
    13621 cggcttccag ttttcgataa gctggaacac cgggctatcg atgggcgccc gatcgatggc
    13681 aatcttgcga agggcgctca gccgcccggt gagcgccctt cgcaaagcag ccgcttgccg
    13741 tggaccgaaa gcgttgtctt tccccaactc gtacggcagc gcgcgaagga aattcacatc
    13801 aaagggaatc ggttgatgct gagcgaaaat cggctgggtg gtacaggggc gggccgtatg
    13861 ccttatacca agctcgtaga acacgtttgc atttgcggtt gtaagatccg cgaccgcgta
    13921 ttcacagagc agcagccgct caaacatcgg cttgtgaatg atgcctccgg ttttctcttc
    13981 gtcggcgcga acggggatca tccccgcccc tttgatagcc ggctcaatcg cgtttctgta
    14041 gattcgattg aaatcgatat ccggaagtcc ggtcggatct ttctttttcc cgaacggcat
    14101 caagacgaag cacagaggtt taggagtagg cattcttctg ccaaacgagc atagcagaac
    14161 agttcatgct gcgaaccgga tgcgatgtgc ccgccggttc aactacgccg gtcgcggtac
    14221 cagaactctt cgatttcttc gagcgttttg ccttttgtct ctggcgtaac gaacaggacg
    14281 aacagaaagg tcaccacgca gaggcccgag tacagccaga acgccccgga gatggagatg
    14341 gcttgtgcca gcgaaaggaa ggtcatcgtc agcgcgacac aggctaccca gaggctgatg
    14401 gtggcaatcg acatggcgcg gccgcgtatc cgtgtgggaa agatttccgc gagcaccacc
    14461 cagacaccgg ggccaagtcc gacggcgaag gcggcgacgc aaaacagcat ggcgggaagc
    14521 accagagccg cggggggcgg tttcagcaaa aacgcgattc ccagcaacaa ttgcgctatt
    14581 gccattacac ccgaagacca catgagcagc cgcttccttc ctgcccggtc gatgagccat
    14641 agcgcaacga tggtggccac aaagtttacc aggcctatga tgacgttcgc tccaatggct
    14701 gctgtttccg tgtgtccgcc cactcgttcc ttgaagatga tggagccgta gaacagcacc
    14761 gtgttaattc cggtgatctg ctgaagcacg gcgagggtaa ccgcaatgag cagggcccgg
    14821 cggagccctg gctgaaacag ttcgcgcagg ctgcccgatt caccggcaat agccgtcttg
    14881 atgtcttcga tttcgcgccg ggcctgcgct tcgcccgaca cgcgcatcag tacccggaac
    14941 gccttgtctt ccgcaccgtt ttccgtgagc cagcgagggc tttccggcac gaacaacaac
    15001 ccggcgaaga acgccgccga aggaaggatg gcggcagcga acatccatcg ccagctctcc
    15061 ggcccatgat aggagagcag ccagttcacc aggtacgcaa gcaaaatacc ggtgacgatg
    15121 gccatctgat tcagcgatac caggcggcca cgaatacgag ccggagagac ttccgcaata
    15181 tacagcggcg ccagcaccga agcgaccccg atcgccagtc ctccggcgaa tcgcgctgcc
    15241 gtaaactccg tcaaatcgcg aggaagcgcc gcaccggccg aagagagtgc gaacagaatc
    15301 gcggagacga tcagtatccg gcggcggcca aagcggtcgc ttgaaatccc cgcaaggctg
    15361 gcgccgaaca cgcatccgat caggagactg ctggcggcca cctccgtctg gaattccgtt
    15421 aatgcgaatt gctggcgcag gaggagaatg gctccattga tgaccgcgat atcgaacccg
    15481 aacagcaggc ccgacgccgc ggccaccagc gaaaccatgt aggcgtagag cgtgtggccc
    15541 tgcgccgcgg cttcaccgga caaggggctg gcgccggcgc ggaaaacgga tggacccggc
    15601 attgacgcgc tcaggcctcg tgaaccactt caaagcccat aagcctcccc atacgttcca
    15661 ccgtcttcac atgatcgccg tagaagatta cgcgatgcaa gcccgcgccg aagttttgaa
    15721 gccacttgcc ggcgccggga acgcgcgtgc gaatctggct gcggcagccg cgatcgctat
    15781 ccaccggacc cgttacctcg gcagtcgata tcagcatctg ggttgggccg ttgaacctcg
    15841 cgaccgtgat ggtctcatcc gaaggcatca acacctgcaa caccgcgcct tcattcgtct
    15901 ccaggtgatt gcggacgatg tagggcgaag ccgggccgtc cggtcccttt agcttggtgg
    15961 cggaaacaca gtgcgcgtga atcacttcgt tgcggctggt atcgaagaca gggtcggaaa
    16021 cgaagcccgg aaccccggca taggaagtta ccagcatttg cgtcatcgtg gaggcaaggt
    16081 caccctcgca gacgccatac aatccggcgt cgttgaattt ggaccaggcg atgcagggat
    16141 agcctggaag cgtgtttgcg gccagcgtgc cgaagcagtc gatcgtgacg gcgtttgctt
    16201 tctccttcaa cagcatctcc ttaaggccca gatagaatcg cacgccgttg aagatctcct
    16261 ctcgtgatgg ctccactact cgtagcgcgc cgcgcacgaa aagatcggtt tcgcccctgg
    16321 ctttttcctt atcaaccgca ttgaaggcgt tatgaaactc ggcgccgttc aggaaccgga
    16381 agctcgtgcc gaaatgccgg ctgtatgcgt ccgcctgctc ctggcgcgcg ccgggagaag
    16441 cgactcccac cagcaccttg ctcttccgca gatggcgcac agtgcggaaa aggcgcatat
    16501 aggggtcgag atctccgtag ctggaggttg cgatcacgtc tatcttctta ccggctttcc
    16561 gaagtccggc aatgctcgac caggcgtgcg tcgcataagg gcgcgagaag aacagcgcgg
    16621 gaacattaag agaatccaca agcgaacgca gcggaccgat gggctggctt atgggaatga
    16681 tgagcacgcc gtcgatatcg cccaccttag ccagccacgg cttcacctcc tcatcggttc
    16741 gtagaagatc gcctccaaca aaccgcacgt tctgttcgtg catgcgggca acttcggcaa
    16801 gacggctctc cacctccccc acttcagcgt gtacatcgag tgtggtcttg ggccagtgga
    16861 ctcgtgtcga cgccagataa accttcgcca ccgtggccgg ggcgttccat ggcatggaag
    16921 aaaattcttc ggcggctgcg ctgaacgata cgaatgggac gccgcacagc gtcaaattga
    16981 atttctggcg tgtgaaacgg gtatcagtgt acataatgac ctcattcaga cgttaacgtc
    17041 tgtttttaga gatggcaaga gctaattcgt gccgctatgt cacaaaccgt tgtttatctc
    17101 tacattttag tataaactta ccttttattc tcttattttt cagatgcctg gcggacgaat
    17161 caaatgaggc ggcttctcct tagcggcgat gaggctgtcg cacttgcggc cttggagtcg
    17221 ggagtgcggc ttgggacggg ctaccccggt acaccctcga cggagatcct cgaagctttt
    17281 tccgggctcg gcggccgtgc gcagtggtcc ccgaacgaaa aagtggcgct cgaagtggcg
    17341 attggcgcgg cgttcggggg agcgcgcgcc ctctgcacca tgaagcatgt ggggttgaac
    17401 gtggcggcgg acccgctgtt cacggtggca tataccggcg tttctggcgg gctggtgatc
    17461 gtttccgcag acgaccccgg catggcgtcc agccagaacg agcaggacaa tcgcaggtac
    17521 gccgaagcgg cgggtgtgcc ggcgctggag ccttcggatt cacaggaggc atacgagttc
    17581 acggcgctgg cgttcgagat ttccgaacgc tgggaaatcc ccgtactgct gcggttgaca
    17641 acgcgcgtct gccactcgaa aacggtgttg cacgtgccgg agtgcgccgc cggcgcggta
    17701 ccgcttccac gcttcacacg agacatacac agccgcgtca tgatccccgc taatgcacgt
    17761 ccggctcacc ggcggctgcg ggcaaaactc caggccgcgc gcgagtggaa tgaagcgagc
    17821 gggccgaacc ggtggatgga gggagaacgg gcgcttggca ttatcgcgtc gggcgccgcg
    17881 tacatgcacg cgcgcgaagc ggccccggcg gcatcctttc tgaaaatcgg aatgacgtac
    17941 cctttgccgt tggatacgat ccgcaagttc gcaacgggcg tcgagcgctg cgtggtgatt
    18001 gaggaaggcg atccatttct tgcggacgcg atccgggcgg cgggtattca ggtggaggcc
    18061 aagccggaga tgtaccggtt cggcgagttg aacgtggagc gcgtccggcg tattctggcg
    18121 aacgatgttt ctcccgaacc gccgctgcct cgggggacac ctcctcagct ttgcccgggg
    18181 tgcccgcatc gcgcggtgtt cagcgcgttg cgaaatcttg gatgcatcgt ctccggcgat
    18241 atcggatgtt actcgctcgg tgtgctaccg ccgtttgaag caatggacac gctggtgtgc
    18301 atgggggcgt cggtcggtgt ggggctcggg cttcgtcacg tgcttccccc cggacagcgg
    18361 cgcaaggtgg tcagcgtaat cggggacagc acgttcgtcc acagcggcat cacgggcctg
    18421 gtggaaatga tctacaaccc gccggaggac ggacacgttc tcatcattct ggacaacggc
    18481 accacggcta tgaccggcat gcaggaacat ccgggtaccg gccgcactct tcagcacacg
    18541 gcgacaggga agatgatctt tgaggacgtg atccgcgctc ttggaatacc gcgcgtgcac
    18601 gtgatcgatc caacgatcaa gccccaggaa tttgagaatc tcatcgcggc gtgccttgat
    18661 tcgctcgatc tgtcggtagt gatcgcacgc cgggactgcc tgctggcggc gagcgccatc
    18721 cggcaatacg aaaaatgcct ggagccggtc catgaggaaa aagggcgaaa accgcttgct
    18781 gacgcgcgcg gctcagtccg agccgcgacc gcgagggagc ggaccccgaa ctcccttttt
    18841 cagcatcctg ccagaaccat caatatcgtt attgcgggac tgggcgggca aggcgtgctc
    18901 aaggcttccg acatcgccgc ggatgccgca ttccgtgccg gcttcgatgt gaagaagagc
    18961 gagttgcatg ggatgagcca gcgcggaggg tcggtgacga gcgatgtgcg tttcggtgaa
    19021 tccgtcctca gtccaatgat ccccgaaggc gaagcggatt tcctggtggc gatctcgccc
    19081 gaacaggtgg aacccaacca tcaccttttg aggccggaag gaacattgat tcatgcgggt
    19141 ctgattgacg caggcgcgct caaaaacagg aaaagcgtga acgtggcact gcttggcaag
    19201 ctcagccgga gcctgcaact gcctgacgag gcctggatgg aagcaatccg ggcaaacctt
    19261 ccggagaaac tgcacgaggt gaacttgaac gcgttcgcgt tggggagaaa agcatgagta
    19321 cgctctgcga ggttatcgcg gcgcgtttcc agccggcgag cgcgccggac tatgtgccga
    19381 gggagcatct gcgggaattg caatcggcgc gcctgcgctc ggttgtggaa cgtacttacg
    19441 agcgggtgca attgttccgg tcgagaatgg accggctggg tcttcaccca caggacattc
    19501 gcgacatcga ggatctcgcc ctccttccgt tcacgacgaa agccgacctg cgcgatacgt
    19561 atccgttcgg cctgttcgcg agccccatgg acgaagtcgt gcgcctgcac gcttcgagcg
    19621 gaacgacggg caagccgatt gtcgtggcct atacgcaaaa ggacctggcg atctggacga
    19681 acgtaatggt aagaacgttt gcggcgcacg gcattcatac cggcgacatc gtccagaacg
    19741 cctacggata cggcctcttc acgggcggcc ttggagcaca ttgcggcgcc gaggctctgg
    19801 gagcgactgt cattccgatt tccggcggaa acaccgaccg gcagatcatg gtgatgaaag
    19861 atttcggcgt gacggtggtc tgctgcacgc catcctactt cctgcacctg ctggaccgtg
    19921 cccgagagct aggggtggat gtgcgctcgc tgccgcttcg ggtgggcgtc ttcggagcgg
    19981 aaccgtggac cgaatcaatg cgcacacgca ttgaggagca gggcggcatc aaagcctacg
    20041 atatttacgg tctctccgaa attatcggcc cgggcgttgc gggcgagtgc atgtgccagg
    20101 ccggcctgca cgtgttcgaa gatcacttct atccggaaat catcgatctc gaaacggagc
    20161 ggccggtgaa agacggtatg gaaggcgaac tggtgctgac gacgcttacc aagtccgcca
    20221 tgccgatgat ccgctatcgc acgcgggaca ttacctgcat cctcaccgaa gcctgcccct
    20281 gcgggcgtac gatgcgccgc atacgaaaga tctcgcgccg gagcgacgac atgttcatca
    20341 tccgcggggt gaacgtgttc ccgtcgcaga ttgagaccgc gctgcttgac gtggaaggaa
    20401 cgctaccgca ttaccagatc attctgaccc gcgcgcaagg gctggatgaa attgaggtgc
    20461 gggtagaagt cacacccgag gttttcagcg ataaaatcgg cgccctcgaa ggactccaca
    20521 gccgcctgat tgaagcgctg gacagggtgc tgggcctgcg cgttcgtatc cggctggtgg
    20581 aaccgcagag cattcaacgc agcgagggca aggcaaagag agtgatcgac cggaggttcg
    20641 aggaccagtg aaaatcgacc agatctcagt cttcctggaa aacaagccgg ggcactcgat
    20701 tgaaccggtg cgtctgctgg cgcgggcagg tatcgatatc aggacgttct cggtggctga
    20761 cacccgcgaa ttcgggatcc tgcgaatgat tgtctcggac ccggagagtg cgacagacct
    20821 gctgcgaaag gtggggtacg tggtgaatct cacggaggtg ctggccgtgg aagtcacaga
    20881 ccggcccgga ggactggccg gggtgctgga ggtgttcgaa aacacggaaa tcaatatcga
    20941 atacctgtac gccttcccgg tccgccgggg cgacaaagca gtactgattt tccggttcga
    21001 caaccccgat gccgccatcg accggttgca acgggccggc atcaacgttg tcgaaacgct
    21061 ggaggtctat cagcattgag ccgatgcctg tatcgaaaac aatcgccgga caactgaaga
    21121 acgcatcctg gattcgcagg atgttcgaag agggcaaccg cctgaaggcg gagcgcgggg
    21181 cggatcgcgt gttcgatttc actctgggaa acccggaggt ggaaccgccg gagcaggtgt
    21241 tggaagcgct gagccgtgcg gtgtcgaacc gaacgcctca ccggcacggc tatatgccga
    21301 acgccgggca catcgaagcg cgcgctgccg tggcacggca gctttccgtg gaaacaggcc
    21361 ttccgtttac gcccgatcac attctgatga ccgtgggggc gtcgggtgcg tgcaacgtca
    21421 ttctgaaatc gattctcgat cccggcgatg aagtgatcgg attaatgccc tgcttttcgg
    21481 agtatccctt ctacgtcacc aatcatgcgg gccggtttgt gccggtggag acggacgcgg
    21541 attttctccc ggacatcgaa cggatcgcgg cggcgattac accacgcacc agggcgatta
    21601 tcctcaactc gccgaacaac ccctccgggc gcatctatcc ggcgggagcg ctggaagaac
    21661 tcgaagaatt gctttcgcag cttgaccacc cgataacggt gatcagcgat gagccgtata
    21721 agagactcat cttcgacggg cggaaacaaa cgcccgcagc ctcacgcatc agccagacgg
    21781 cgatctgcaa ctcgtggtcg aaatcacagg cgattcccgg acaacgaatc gggtatctcg
    21841 cgctttcgcc gcgcctcgcg ggattggaag aaattcgaaa tgcctgcact ttcgcgaacc
    21901 gtattctcgg attcatcaat gcaccggcga tctggcaatg ggtggaggcg gaagccgcgg
    21961 aggcagtggt ggacgtgctc gcttatcagg agaaacgtga ccggctgtgc aacgcgctga
    22021 cggccatcgg ttacgacgtc cagaaaccgg aagggacgtt ctatgttttt ccgaagactc
    22081 cggtttcaga cgatgtggaa ttcgtcagat tgctgctaaa cgaaggagtg ctcggcgtgc
    22141 cgggatcggg tttcgggcga agcggctact tccgcctgtc cttgacggtg cccatggaga
    22201 ccataacaaa ttcgctgccc gcctttgaac gagtctttca ccaatgcaaa gaagcacggc
    22261 cttccgccga ctcctttcag ccatcgcgct agggtcctgt gttctgcccg ggcaagccca
    22321 gcagaatata gagccgcgcc tgttcgaata cggttttgaa cagcgggtgc gaacggagaa
    22381 ctggaacaat attctcgatt acgacgccgc ccgtgccgat gagcggcgcc agatccgcta
    22441 tcgctcgaaa ctatggatga aatcgcccgt cacttccgat atcgatgtgt tcgtggcgct
    22501 gaatcaggaa accaaccaga ttctggttcc cgatgttccc cagcggttcg acgaggtaat
    22561 ggtcgaaaac gcttatgtcg attttcgcaa actgttcgtg aaaggactat cgcttcgtgt
    22621 aggcaggcag aacctgatgc gcggagaagg tttcctgctg ctggatggaa gcccgggcga
    22681 cggctcgcgc tcgatttact tcaacgccgc ggtgcttggt tactcgtgga gtaagtcgaa
    22741 gctggaggcg atggcgattc ataatccatc taatgacagg tatcttccaa ggttacatgc
    22801 gagcacaaca cggcaactgg cggaaagaaa tgagcaggcg ctggctctct attacactaa
    22861 cgatcaagtt agtaacacct ctatcgatgc ttactacttc ttaaaacggg aaacccggga
    22921 ttgccgtgcc gccacgcagc accagtacca ggccgaccgc catgtccata ccgccggtgg
    22981 gcggctgaca cgaagaatgc aaggtggttg gagtgcagcc tctgaatttg cggcgcaatg
    23041 gggacgcgaa gagccggggg agcggattgc ggcgtggggc ggctacgggt atttgaaaaa
    23101 gacatttgca cggcccggcg atccttacgt gcttacgggc tactggggta tgtcgggaga
    23161 cgaccccatg acaagcaccc gttatgaagg gtgggatccg ctattcgcgc ggtggcctaa
    23221 gtggagcgac ttgtatattt atagttacat tcgcgaacaa ggagtcagtt actggactaa
    23281 tacctggtta tggcagcttg aagccggtta ttctccatgg aaacgcataa agacaagctt
    23341 cacctgctat gacatgaatg ccttccatcc ctttaacgcc gaccctttga attattcaga
    23401 cggcaaacac cgcggcagac agtaccaggc gcgggcggaa cttagtgtga acgaccactg
    23461 gcgcggtcat attctttatg agcggcatct tcccggaagc ttccatgccg ggcactccag
    23521 cggctggttc ttccgcatgg aagtaatcta tagttttcag ggaaagatcc cgctacagca
    23581 ttgaagtagc ggccgcggca cggctatcct ggatgggccg ccaccacgac aggaggatag
    23641 ccatgtgcaa tcgtattttc gcgctgagtt tgctcgtatt tgcgctgccc gcactcccgc
    23701 agcagaccag gaccgaggta acgaaagcga cgacgcccaa agacgactcc aagcccaaca
    23761 gcgacaaggt gccggaggtc tatgccattt ccggacaatt tgaacgggta gtggtgcttc
    23821 gttataagtt cggcacggac ctgctggccg ggttggaaaa gatggtgaaa gagcagaaca
    23881 ttcgaaacgc ggtgattctg gccggcgcgg ggtcggtccg gaattatcac gttcactccg
    23941 taagcaacag gacgttccca tcgaaaaaca tctacgtaaa ggaccccacg gcatccgcgg
    24001 atatcgtgag catgaacgga tacatcatcg acggccgcgt acatgctcac atgacaatga
    24061 ccaacagcga gggagctttc ggagggcacc tggaggctgg aacgaatgtt tttacgttcg
    24121 caattatcac tctcggcgtc ttcaacgaca gtgtcgattt gagccgggtc gacgacaaga
    24181 cctatcggtg acgctgttac gccaggtctc tcacgaattc ccgcaccgcc tcgccgattt
    24241 cctctggcga gtcctcctgc acgtagtgaa tccccctgac cgtgacctcg cgttgattcg
    24301 gccaggtgcg gcagaattcg cgcgctcgcg gtccgatgag cgctccaggc tccgccgcga
    24361 tgaaaagctt cggaactgcg ctccgcacca gcgtcggaag ccttgcctcg cgctctctcg
    24421 gcaaggcctt tcgcaactac gaacccgcca tcgatctctt ccccgccgat ctccgggcgc
    24481 gtgtcgccgg ccgtgttctc cgccgcgcgc agcaggctct cgacttgatc gcacgcatcc
    24541 tgctcgatcc cggcgattgc gtctggctgg aggatcctgg ctatcccggc gcgcgttctg
    24601 tcttccacgc caccggcgcg cacatcatcc cggttccggt cgatcgcgac ggcctcgatg
    24661 tcgccgccgc ccgcgctctt gcggcctcca cccgtgtggt ctacaccaca ccagcccacc
    24721 acttcccact cggcgtgact atgccgctcg aacgccgcct cgctctgctg aactgggcca
    24781 tcaacgccgg tgcatggatc gtcgaggacg agtacgacgc cgaataccgc tacttcggac
    24841 gacctgtcgc cgtgctgcaa ggcctggacc gctcaggctc ggttatctac gtcggcacgt
    24901 tcaccaaaat gctcttcaac tcgcttcgtc ttggattcct ggtgctcccc gaacgcctcg
    24961 ccggggcttt cgaagccatg cgcagtttcg tcgaccgcca cccgcccacg ctcgatcagg
    25021 ccatcctcgc tgagttcatt ctggaaggcc gctttggcca caacgtccgc cgcatgcgcc
    25081 agacctacgc cgagcgcatg gctgttctga agcaagccgc gcagaggcat ctcgccggac
    25141 tgctcgatgt cgaaaacgcc gccgccggca tgcacaccat cgcctggatc aaaacaggcg
    25201 cacccgacac ggtccttgcc gcccgcgccc gggaccgcgg cctcgaactc agcgccctct
    25261 ccagcttttc cgtccgccac taccatcccc ctgccctcgt tctcggtttc gccggcagca
    25321 gtccttcgga actccgccgg ggtgttcgcg tgctggcttc ggccctgaca tagcgatagc
    25381 tcttgccgga atggtgcttt tcggttatat tcaatgaacg gggtttcccc gaatccttca
    25441 gggatgtacc tgccgatgcg aacaaacgtc agtgtagccg tgcgcgaccg gctctaatga
    25501 gcacgggcgc gtagttgcgc taaaccttcc atctcaaagt tcatagtgtc cgacggcggt
    25561 ggtgtgcgca tgctcgaatg cgcacggccg ggcaaggagt tcgtaatggc tacaactgtt
    25621 tccaaatatc cgcgcgcggg cctctggcaa tcggtaaagg aggcgatccg cgggaccgag
    25681 tacgactata cagagctgcc catcacgcac agtgtgtttc tgctcgctat cccgatggtg
    25741 ctcgagatgg cgatggagtc gctgttcgcg gtcgtggacg tattctgggt gtcccggctc
    25801 ggcgcaaacg cgatcgccac ggtcgggctg actgaatcga tgcttacgct ggtgttcgcc
    25861 gtggccatgg gcgtcagtct ttcgaccacg gccatggtgg cacggcgcat cggtgagcac
    25921 gaccagcaag gagccagggt ggccgccgct caagcgatcc tgctcggtgc gggattggcg
    25981 gtagcgatgg gtgtgcccgg gttctggctg gcgccgcgac tgctcgaatg gatgggcggc
    26041 ccgccggagc tggtggagac agggcaacac tatacggcgc tggtgctggg cggaagtatc
    26101 actgtaatgc tgctgttcct gaacaacgcc atcttccgtg gagcgggaga tgcggccgtc
    26161 gccatgcgcg tgctgtggtt ttcgaacctg atcaatatgg cgctcgaccc gttttttatc
    26221 ttcggatggg ggccctttcc cgagatgggc gttacaggag cggcggtggc cacggtgatt
    26281 ggaagaagca gcggggtgct gtaccagttt tggattctgg cgagccggca aagccgcatt
    26341 cagctccggt ggaccgacat gcggctgaac tggccggtga tgttgtcgct gattcgtgtg
    26401 tccacgacag gagttctaca atttgccgtg gcacatacga gttggatttt actggtccgg
    26461 atcatcagca ccttcggaag tgccgcggtg gcagggtaca cggtgggcat ccgcatcttc
    26521 gccttcgcca ttctgccatc gtggggactg agcggcgcgg ccgcaacgat ggtaggccag
    26581 aacctgggcg cacagaaacc cgggcgcgcc gaacgcgccg tgtggttgac cgggttctac
    26641 aacatgatct atctcggcgc agtcgccatc gtgttcatcg ttctccccgc ccctctggta
    26701 cggctgttta cgtccgatcc ggaggtgatt gccgcggcgg tgaactgtct tcgcatcgtg
    26761 gcgatcggaa atatcgcgta tgcctacggt atggcgatgg tgcaggcgtt taacggcgcg
    26821 ggagataccg tgacgccgac catcatcaac attttcggat tctggctgtg cgagattccg
    26881 cttgcctgga ccctggcact accaggcggg atgggtgtaa atggcgtgtt cgcttccatt
    26941 ccgatttcgg aaggcttgat tacggtcatc agcctgattg tgttccggcg cggcaagtgg
    27001 aagcagcgaa agatctgaaa gcagcgggcg cgccggagca gcgcccgctt ccattcagtt
    27061 atcgccgata tggcaggtag ccagggcgag cagcgggaag tactgcctgt agaggtgata
    27121 cgtgaggtaa aagacgttgg ggaaaccggt acccgtggtg agtttctcgt cccacgtgcc
    27181 gtcgggacgc tgtgtgtcga tcaggtatcg caagccgcga tggacagcgg aagactggcc
    27241 ggcgttggcc gcggaaagtc caagcagagc ccatgccgtc tgcgaaggag tgctggaagc
    27301 agggacgtaa tagtgtcgcg cataacttgc acagctctcg ccccagccgc catcttcgtt
    27361 ctgaatggac tgtaaccagg cggcagctct ttgcatggat gggcgagccg acagatcgcc
    27421 gctggcgcgc aaaccacgaa cggcaagaaa tgttccatag atgtagttca cgccccaacg
    27481 tccataccaa ctcccgtccg gtttctggtt ggcaatgaga tagcgtaccg cgttccgaac
    27541 ggctggatgc ccggttttga ggccgcgccg gcagagggct tcgagaacgc ggccggtgat
    27601 gtcgggacag gtgggatcga gcatcgcgtt gtggtcggcg aaagggactt tgttcagaaa
    27661 ctcccagtta ttgtcgacgt cgaaggcggc ccatccccca tcagaggact gcataccaag
    27721 gagccattcg atggcgcggc gctcgacccg ttcctggcgc tcgggatcag acgcacgcgc
    27781 gtgctgaagc gccagtaaga ccatggcggt gtcgtcgata tcgggatagt attcgttagc
    27841 gaactcgaag gcccatccac tgggccgcag gtcgggacgc ttaatggacc agtctccctt
    27901 gcggcgcact tctttatcca gcagccagtc ggcggcgcgg gtcatcgcgg ccgcatcgac
    27961 gggaccgccg ggcatctctc ccacggcaaa ggcggctatc gccgtatccc atacgggcga
    28021 aaaacagggc tgaaactgca aacggtcttc cgtctcgagc agcaaccggt caaactgcgc
    28081 gatggcttca atcagatcgg ggtgatcgcg ctcatagccc aacgcatcca tcgccatgat
    28141 ggtatacatc atggcgggat agatggcgcc taagccgtcc gcgtggtgca agcgctccaa
    28201 aacccatcgt tccgcctcac gcacggcggt tttgcggacg tccttgattc ccctccgctc
    28261 ccacattttc accaggcggt cggcatggtt gaatacggtg gaggcacgat ctcttcgcgg
    28321 cagggtcaga cgcttaccgg gaacaaacaa ctcctccacc gtgagactgt gaggaacagg
    28381 gtggtggccg cctatagact ggacgatcga aagcggaacg agaatggtgc gtgtccagga
    28441 agacatttca tatagaagat ttccgggaac aagcagaatc tcgggcggca cactgggcac
    28501 ataccgtttg ggataaagcc cgaagagact gagattcatc ttggtgtagc tattcccggc
    28561 ctggaggccg cccagcgaaa ggacacggct gcgtgcccgg accatcgcgg ggctttcagg
    28621 gtcttcgccc gcaattttca ttgcggtata agcgcgagcg gtggcgttga cctccgacgg
    28681 accaccttcg taaatggacc atccgccatc gggcaactgg cgctcgagga tgaatcgcac
    28741 cgctttccgg atgcgattcc aggccggggg acgccacggc tgatccggtt ccggcggatg
    28801 cagccagagc tgaagaagaa tatagtcgga ttccaaagtg gagtcggccg taaggtcgcc
    28861 gcaccagtat ccttgcggat cctgaatctt aagaagagcg ttggcggcgg ccgaggcgct
    28921 tgcctgtatg cccgccgcaa gctgctccaa ctcagcctga gcgattgtgc gagcggcctc
    28981 tgtgtcgcgc ggcggtgccg gcgcgatata tcggaattct tctctcatga tcgaaaagat
    29041 aaagttttct cagaaatctc gcggtgtctg tggcaggtca ccagatgatc gttgaccatc
    29101 cccaccgcct gcataaacgc ataacaaatc gtagagccca cgaatcgaaa ttcccgtttc
    29161 gcgagatctt tgctcatggc atccgatact ggcgtccggg cgggcacctc gtcaaccgtg
    29221 cgccacgcgt tcacaacagg cgtaccgttc acgaattgcc agatgtagcg gtcgaacgtt
    29281 ccaaattcat cgatcacatc caggaacgaa cgtgcattcc gcaccgccgc cgaaatcttc
    29341 agacgattgc gaacgatacc ggagttggcg agcaattcgc cgatcttccg ctcttcatag
    29401 cgtgcgatga cagcgggatc gaacccatcg aaagcggaac gatagttctc gcgcttcttc
    29461 agtatcgtcg cccagctcaa acctgcctga gcgccttcga gaatcaggaa ttcaaacaaa
    29521 acacgatcgt cgtgttgcgg tacaccccat tccgaatcgt gatacaagat atccagatcg
    29581 cttttcgccc aggggcagcg cttcttcacg tgctcgtgca attcctattt tatcgtacct
    29641 ccgtattctc aacggattcg ctctatcctg gaagatacag acgtgattgg accgaaactc
    29701 ttcacagcat gttttctgtt cttcctgctc caacaaagtg caaatgcgtt cgacgccgag
    29761 gcacgtatac gcgcggaagc ggcattccgg gagggagtgg cagcggaatc gacaggcgat
    29821 aacgcccgcg cgatgagggc gttcacgcag gcgctggaag ccgaccccga ctacgggcca
    29881 gcatggctgc gccggggacg tttgcaactg aaggcggccg actacaaaca ggcgattgca
    29941 gatctgaacc tggcggtcgc ggcggggctg gagaatgccg acctctatga gacgcgaggg
    30001 gcggcgtact cacagctgga gcactggaaa tacgcgatcg cagattacac ccgctgcgtt
    30061 gaagccgacg cgaaacgaac agcctgctgg accggacgcg gcttcgccta cgggcagatc
    30121 gagcaattcg gaagcgcggc acgggatctg acccgtgccc tggaactggg ggacagcgat
    30181 ccgagaacct atgcgctgcg cggcgcggca tatttcaaga ccggcgagat ggaaaaagcc
    30241 atagccgatc tcacaaaagc gctggaaagc cagccgaacg atccgttgct gtatttagtt
    30301 cgcgcggctg catatgcgga gacaggcgat caacaacagg ccctacgaga ccgcgagcag
    30361 gcggtccggt tggattctcg aaacgcggaa gcctatgtag cgcgcggggc atcgtatcac
    30421 gcgctgggcc gtcacgaaga aggatttgcg gatcgtacaa gagcgcttgg gctgaatccg
    30481 cagtttgccg aggcctacct cggacgcggc aacgcgcacc tgtcgctggg acaatacgct
    30541 gaagccgttg aagacctgca gaagctggtg gagttgaaac cggacgatgc cgaggcacgg
    30601 gcgttgttgc aaagagcgct ggacgctcaa cagaaggcaa ccgaggcgcc caaaccaatt
    30661 ccgcaagaga cggcaggggg cgggaaggag accgcggttt cgaaggcagt cccggcatca
    30721 ggcgcggcgc ttgcccgcaa gcattacgag acgggaagga agttactgga ccaggcgaag
    30781 ttcagcgcgg ccgtggacga gttgtccaaa gcaatcgccc tgaatgcgaa aaatgccttc
    30841 gcgtacaacg cgagaggata cgcatacctg cgactgaaac aatataagca ggccatagcg
    30901 gactttgacg cggccctacg gctgaatccg cgctatcgca acgcctccag gaatcgggag
    30961 gcggcccggc gcgccgctca aacaccccag cagtagttga catatgacga ctccacggag
    31021 cgggtgtacg gaaatggctg taaacggaca tttgataatg tggaatcgga tgcgccgcga
    31081 tgaaaaaacc tcgtaaagac ccggtgcggg aaaaccgaat ccatgaggag gccatcgtgg
    31141 acgcttacgg gccggaagaa caggcgatga gttggtactg ctaccttgaa gggaaactca
    31201 gcttcccgtt cccagcgaca tgcacagctt cgaagattac ctcgccgttg aaaaagggtg
    31261 aaacggttga tgtgctccgc atggcacccg acgaagcctg tacgacggac atgctcgtgt
    31321 tggtccgccg gcagggcaga aaactcgcgg ttccgctcgc ccagctcaag cctgttgacc
    31381 cggacgaatc aaccgacgaa gcggccggcg actggcacta ctgggttgcg caaggctacc
    31441 ggttctgatt gtcgtccctc acgcacgatc gctgaatccg cgctatcgca acgccgccag
    31501 gaacagggag gcggccaggc gtgccgctca aacaccccag cagtaatctt cgacgacgcg
    31561 ctcgacgctg ga
//
LOCUS       DASTMK010000302        12689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_302889, whole genome shotgun sequence.
ACCESSION   DASTMK010000302 DASTMK010000000
VERSION     DASTMK010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302889"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="VFQ79_13200"
     CDS             <1..1601
                     /locus_tag="VFQ79_13200"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEU0140671.1"
                     /translation="VGPSADGFETYGVTGVALITFILLAVPNPIVQVQLLVWVFVTRV
                     VMLIASGLSYFINESIARAKYGHATKFNFEAPLTSLVWLTSIVSVTLTYISAYLLIPD
                     LAGDTSLWWKLSTIITCGTLAGAIIPEFVKVFTSTTSSHVKEVVTASRQGGASLNILS
                     GLVAGNFSAYWLGLAILLLMGIAYGVSLMGMGALMMAPAVFAFGLVAFGFLGMGPVTI
                     AVDSYGPVTDNAQSVFELSTIEQIPGIKQEIKSQFGFEPDFEKAKDNLEENDGAGNTF
                     KATAKPVLIGTAVVGATTLIFSIIVMLTHGLQPELLRFLSILHPPFLLGLIAGGAVIY
                     WFTGASIQAVSTGAYRAVEFIKANIKLDDTVSKASVEDSKRVVEICTRYAQKGMFNIF
                     LVVFFSTLACAFLEPYFFIGYLISIALFGLYQAIFMANAGGAWDNAKKIVETELKAKG
                     TELHAATVVGDTVGDPFKDTSSVALNPIIKFTTLFGLLAVELAVSLSAQQGHGVSIAL
                     AAIFFAVSMVFVRRSFYGMRIEEK"
     gene            complement(1659..3455)
                     /locus_tag="VFQ79_13205"
     CDS             complement(1659..3455)
                     /locus_tag="VFQ79_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU0140672.1"
                     /translation="MIRCAFLLFALIAYSLAQEVPRPEYPQPQFQREQWLSLNGPWEF
                     EFDDANAGLEQNWGNGQKKFSKTILVPFSFETSKSGIRDTSFHPWVWYRRTFNVPSQW
                     KQKRVLLHFGAVDYRAMVWVNGQKAGSHEGGHVPFRFDITPLLRDGANVLVVRAEDPP
                     RDRFIPRGKQYWEPESRGIFYTRTTGIWQPVWLEAAGTSYLERVRITPTNDGVVRFDA
                     RIVNPGGDLSFYAIVSLEGRQSALAMVRTSENRAAAAARIESPRLWSPNSPNLYDVTF
                     ELRRGDAVVDRVQSYFGFRSVSVENGRVHLNGRPIYLKMVLDQGYWPESTITPPTDDA
                     IQYDIRLTKEMGFNGARKHQKLEDPRFLYWADRMGLLVSSEMANAYEFDDAYVDRFTR
                     EWIEAVERDYNHPSIILWVPINESWGTPNLRDPKQQQHLRALYTLTHSLDSTRPVIDN
                     DGWEHTDMTDLFALHDYARTGEMLAEKYKDLGKPGAAVPDVSRASLVPGFSYNGSPFI
                     LSEFGGIAYIPAGQQVPKESWGYSGIEKTQEAALVRLRGLYEGIAKVPAWVGICYTQL
                     TDVEQEVNGLLTYDRKPKFDPKLIREINELLK"
     gene            3561..4022
                     /locus_tag="VFQ79_13210"
     CDS             3561..4022
                     /locus_tag="VFQ79_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140673.1"
                     /translation="MPFCTQCGNAVRDTDIYCGTCGTRQGAGPSGGGSGSQIASGLSP
                     RTAALLCYVPIVGWIASIVVLAAAKFRTDRDVRFHAFQGLYLFVAWLIVDWVVAPFFT
                     FVPRHMLPVGSILKLAIIGTWIFMLVKTAQEQMFRLPIIGELAERSVAEQK"
     gene            4354..4701
                     /locus_tag="VFQ79_13215"
     CDS             4354..4701
                     /locus_tag="VFQ79_13215"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU0140674.1"
                     /translation="MRDFMEYKGYIGSVHYSGEDEVFHGKLQGIRDLITYEGTDVGGL
                     KQSFYEAVDDYLADCKKRGKAPDRPFKGTFNVRVGRELHKRAAVFASERKRKLNSVVS
                     EALEKYLETADCG"
     gene            4805..5812
                     /gene="argC"
                     /locus_tag="VFQ79_13220"
     CDS             4805..5812
                     /gene="argC"
                     /locus_tag="VFQ79_13220"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU0140675.1"
                     /translation="MKHTVGIVGFRGYSGMELLRILERHPHIHPVLLEHRTDVGEQSN
                     LRRKAGPPRLPCTAEAAKEHGLAAVLLATPADVSMGLARTFLDAGIKVVDLSGAFRLQ
                     SPENYHRWYKEEHTEPALLAEAAYGLPEFCRSCIPGARLVANPGCYPTAANLALKPLV
                     AAGVIDRPAGIVCDAKSGVSGAGRKPSLKTSFCAVTENFSAYSILEHRHVPEVLMYSG
                     IEEAEFSFTAQLLPLDRGILETIYFRSAKLESAAQLIDIYEAQYGEEPFVRLYEAGRV
                     PDLRSVQHTNYCDIGVKFDGTTGRAVVVAAIDNLVKGAAGQAIQNLNLILKRPETEGL
                     L"
     gene            5809..6591
                     /gene="argB"
                     /locus_tag="VFQ79_13225"
     CDS             5809..6591
                     /gene="argB"
                     /locus_tag="VFQ79_13225"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU0140676.1"
                     /translation="MRILIKLGGTLLDSDDSRRRLAREIAALGRTHSVVVVHGGGKQM
                     TRFLAERGVESRFVEGLRVTTPEVLDAVLKVLAGTVNKQLVSALIEAGGDAVGISGID
                     GCLTEAETLRPELGAVGRPLRTNPAVLNALSGAGFIPVVACVAGDRQGRMFNVNADQM
                     AVSCAAGFGAERLLFLTDVEGVRGAGGAVLGSLTAGDCIRLVRDGTATGGMQAKLNAA
                     VQALEQGVGEVVILPGYEPEILDRALRGEPLGTRLIPEEVRR"
     gene            6588..7109
                     /locus_tag="VFQ79_13230"
     CDS             6588..7109
                     /locus_tag="VFQ79_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU0140677.1"
                     /translation="MNTLVTLAPPLERAAPPFVENAPVEIRMARMSDVRALLDLINGY
                     AAEAIMLPRTEFELAENIRDFTVAAVGGDVVGCAALHFYSAATAEVRSLAVAPQWKGH
                     SVGRLLVESLEKEARNYGLASLFAFTYVPAFFSKLGFVEVERGELPLKAWKDCLRCPK
                     FQCCDEIAMIKKL"
     gene            7384..8355
                     /locus_tag="VFQ79_13235"
     CDS             7384..8355
                     /locus_tag="VFQ79_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU0140678.1"
                     /translation="MRAQTVDVRESSGKVLCCTIFRAGGKKLLAKGHVISEEDIRLLE
                     TEGMDQVWVTVLEEGEVAEDDAVCAVAQEMSCGCSEIRLAAGGRANLVATEDCCVLVD
                     DELLKQINCTASIVIATAPNFSHAKAGQRIATVKSAPFAVAQSQLEAVISILNERGPI
                     LQARPMRTPKVAVLYTDPASGDRSRQLFENVMRQRLERFGTTASFVLSAVEDEETVAK
                     SLHHLARAKPAVILVASTTAPAGPEDVVGRAMTKVGCHLERFMAPVEPGNLLLLGYLD
                     DLPIVSAPGCFRSAKLNVMDLILPPMLAKYRVSGWELACLGHGGLLA"
     gene            8545..9747
                     /locus_tag="VFQ79_13240"
     CDS             8545..9747
                     /locus_tag="VFQ79_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0140679.1"
                     /translation="MKVTKVQPFLLSYPFDTPVRLSYHGGERTIHKRDAMLIRIETDQ
                     GLVGYAPGQGSEAAHAAIQDSIAPFLEGRTLADPDALRVLFLKGPGRNPLLTRVYCAV
                     EIGLYDLIGKQKGLPVSELLGGRVRDRIRLYGSAGMYMPPEKYAEEALTVANLGFRAY
                     KMRPALGEQKDLETVRLMREAVGSDFDLMVDAHTWWRMGDRSYSFETVQRLARDMAEY
                     DIAWLEEPLPPDDHEAYRTLHDLDYVPLASGEHEPDEPGFMDLIQSGGVDYVQMDVIC
                     QGGYPTGRRIFGEIARQGLRFAFHSWGTALEVIAAAHLGICWPETVADWLEYPCYSTP
                     SRAGMYPFPLAAEILKEPLEIDGGDLIVPRKPGLGVEIDESIITRYPWIPGPWSYFRW
                     ILLPGHGR"
     gene            9767..10480
                     /locus_tag="VFQ79_13245"
     CDS             9767..10480
                     /locus_tag="VFQ79_13245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0140680.1"
                     /translation="MDGRRAGCLKAICSTAILGAGAALAWGVRGRSSALFAPSVYRGR
                     RDRPAIALTFDDGPSESTPVLLELLARYSVPATFFQCGLNAARLPRVAREVALAGHEV
                     GNHSHTHRLFPCLTPAEVREDLARAQRAIEDATGVTPRLFRAPYGARWFGLRRAQREL
                     GLLGVMWTSIALDWKLPSAAITRRILRAASNGAILCLHDGRELRIDPDIRNTATALRG
                     AIPALAARGFCFETVSNIL"
     gene            10477..10740
                     /locus_tag="VFQ79_13250"
     CDS             10477..10740
                     /locus_tag="VFQ79_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0140681.1"
                     /translation="MNDELTQRVIKIIAATQHIELEKITPESTFQELGIDSLDGINIL
                     FALENEFSINIPDDAGQRIRTVREMVDGVRTLISGGDPAQAQA"
     gene            10742..11962
                     /locus_tag="VFQ79_13255"
     CDS             10742..11962
                     /locus_tag="VFQ79_13255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU0140682.1"
                     /translation="MPGRRIVITGLGAICALGRNKDEFWDSLVRGVCGIRPLETVDRT
                     QFRVQNGAEVLGYDSAEHFPGKEREQLDRFAQFAVTTGREAIADSGIEWQDGQRERTA
                     IVTGSCVGGQATENEGYVNLYLLKNSRVNPLTIPRSMENAGASRLSLEFGITGPVYTV
                     STACSSSNHAIGQAFWLLRQGVCDIALAGGSEAPFGTGMLKAWEAMRVLAPDTCRPFS
                     KDRRGLVLGEGGAMLVMETLEAARARGARIYAEILGFGMSSDAHHLTQPAAEGAARAM
                     RSALADGCLNPSQVGYINAHGTGTLANDPTEVAAIRSVFNSHASDMAVSSTKSMHGHA
                     LGAAGAIEAVATILALDRGLLPPTANFTERDPQCDIDVIPNTAREQKVDYALSNSFAF
                     GGLNAVLVFGRLRG"
     gene            complement(11966..12586)
                     /locus_tag="VFQ79_13260"
     CDS             complement(11966..12586)
                     /locus_tag="VFQ79_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140683.1"
                     /translation="MKALALLLITAAVLAAQSDYPKHNIPLGLGGAQPRGDLKPFYDT
                     SFVLGAGYGYRFHRYLQADVGLDTAFGAAGVREFLDSGFGYLRIRDFQYFVPFGGRVI
                     APLADGRLLISGGAGGAYLRYSERIRQPSDYYQFDCPVCTSRSGWGYYGLFGVNWFLD
                     QGRHFRIGAVTRVYRGHTEGDPVGELSALRTRDRWVNVFGEFGFSF"
BASE COUNT         2535 a         3556 c         3938 g         2660 t
ORIGIN      
        1 cggttggccc cagcgcggac gggtttgaaa cttacggtgt cacgggcgta gccctgatta
       61 cgttcatcct cctggcggtg cccaatccga tcgtgcaggt tcaactgctg gtgtgggtgt
      121 tcgtcacgcg cgtggtcatg ctcatcgctt ccgggttgtc gtactttatc aacgaatcga
      181 ttgcccgcgc gaaatacggg cacgccacca agttcaattt cgaagctccg ctgacttcgc
      241 tggtctggct gacttccatc gtttcggtaa cgttgaccta catcagcgct tatctgttga
      301 ttccagacct ggcgggcgac accagcctgt ggtggaagct ttcgacgatc attacctgcg
      361 gcaccctggc cggcgccatt atccccgagt tcgtgaaggt gttcacgtcc actacatcct
      421 cccacgtgaa agaagtggtg accgcatcca gacagggtgg cgcgtcactg aatatcctgt
      481 ctggcctcgt ggccggaaac ttcagcgctt actggcttgg tttggcgatt ctgctgctga
      541 tggggatcgc ctacggcgta agcctgatgg gaatgggcgc cctgatgatg gcccctgcgg
      601 tattcgcgtt cggtcttgtc gctttcggct ttctcggaat gggacccgtc accatcgccg
      661 tggattctta tggtccggtg acggacaacg cgcaatcggt atttgagctt tccacgatcg
      721 agcagatacc tggcatcaaa caggaaatca agagccagtt cggctttgaa cccgatttcg
      781 aaaaggccaa ggacaacctg gaagagaacg acggagcggg caacactttc aaagcgacgg
      841 ccaagccggt gttgattgga accgccgtgg tgggtgcaac tacgctgatc ttttcgatta
      901 tcgtgatgct cacgcacgga ctccagcctg aattgctgcg attcctttcg attctccatc
      961 cgccgttttt gcttgggttg attgcgggag gcgcggtgat ttactggttc actggcgcat
     1021 ccatacaggc ggtcagcacc ggggcatacc gtgcagtgga attcatcaaa gccaatatca
     1081 agctggacga cacggtttcc aaagcctcgg tcgaggacag caagcgggtg gtcgagatct
     1141 gcacgcgcta cgctcagaag ggcatgttca acatctttct ggttgtgttc ttctccactc
     1201 tcgcatgcgc gttcctggag ccgtacttct tcatcggata cctgatctcc atcgcgttgt
     1261 tcggcctcta ccaggccatc ttcatggcca atgcgggcgg cgcttgggac aacgcgaaaa
     1321 agatcgtcga aaccgaactg aaagccaaag gcaccgagct gcacgcggct acggtggttg
     1381 gcgatacggt gggcgatccg ttcaaagata cgtcgtcggt ggcgctgaac ccgatcatca
     1441 agttcaccac cctgttcggt ctgctggcgg tggaactggc tgtctccctc tcggcccagc
     1501 aggggcatgg cgtgagtatc gctctggcgg ctatcttctt cgcagtctcg atggtctttg
     1561 tccgcaggtc gttctacgga atgcggatcg aagagaagta gatctggcgt ggaggggcgg
     1621 cgtgggccgc tcctccacaa caacccttca cgagtccgtt acttcagcag ctcgttaatt
     1681 tcacgaatca gcttcggatc gaacttgggc ttccggtcat aggtgagtag gccatttacc
     1741 tcctgctcca catccgtaag ctgcgtatag cagattccca cccatgccgg cacctttgcg
     1801 attccctcgt aaagaccgcg caaacgcacg agcgccgctt cctgcgtctt ctctatgccc
     1861 gagtaccccc aggattcttt cggtacctgt tggccggcgg gaatataggc gatgcccccg
     1921 aattccgaaa gaatgaaggg cgacccatta taggagaatc cgggaaccag tgacgcgcgg
     1981 ctcacgtcgg gcaccgccgc ccccggtttg ccaagatcct tgtacttttc agcaagcatt
     2041 tcgccggttc gcgcgtaatc gtgaagagca aacaggtccg tcatgtcggt atgctcccag
     2101 ccgtcgttgt cgatcaccgg ccgtgtagag tccagcgaat gcgtcagagt gtacagcgcc
     2161 cgcagatgct gctgttgttt ggggtcgcgc aggttcggag tgccccaact ctcattgata
     2221 gggacccaca ggatgatgga gggatggtta taatcccgct cgacggcctc tatccattcg
     2281 cgagtgaacc ggtctacgta ggcatcgtcg aactcgtagg cgttcgccat ctcgctcgat
     2341 accagcaagc ccatgcggtc cgcccagtaa aggaatctcg gatcttcgag cttctgatgt
     2401 ttgcgcgcgc cgttgaaccc catctccttg gtgaggcgta tgtcgtattg aatggcgtcg
     2461 tccgtgggag gcgtaatcgt gctttcgggc cagtagccct ggtcgagcac cattttgagg
     2521 tagatgggtc tgccgttcag gtgaacccgc ccgttctcga cgctgaccga acggaatccg
     2581 aaatacgact ggacgcggtc caccaccgcg tcgccgcgcc gcagttcgaa ggtgacatcg
     2641 tacaggttcg gcgagttcgg cgaccagagc cgcggcgatt cgatacgtgc cgccgcagcg
     2701 gcgcggttct ccgacgtgcg gaccatcgcc agcgccgact gccggccttc caaagacaca
     2761 atcgcataga agctgaggtc cccgccgggg ttgacgattc gcgcatcgaa tcgcactacg
     2821 ccgtcgttgg taggcgttat ccggacccgc tccaggtagc tggttccagc cgcttccagc
     2881 cacaccggct gccagatccc cgtggttcgc gtatagaaga tgccgcgcga ttccggctcc
     2941 cagtactgct tgccgcgcgg aatgaaccgg tctcgtggcg ggtcctccgc ccggaccacc
     3001 aggacattcg cgccgtcgcg caacagaggt gtgatatcga aacgaaacgg aacatgaccg
     3061 ccttcatggc tgccggcttt ctgtccgttc acccaaacca tggcccggta atcgacagcg
     3121 ccgaaatgca gaaggactcg cttttgtttc cattgtgacg gcacgttgaa ggtgcgcctg
     3181 taccagaccc agggatgaaa cgatgtgtcg cgaatgccgc tcttgctggt ttcgaagctg
     3241 aagggaacca ggatcgtctt gctgaacttc ttctggccgt taccccagtt ctgttcaagg
     3301 ccggcgtttg catcgtcgaa ctcgaactcc cacgggccgt tcaggctcag ccactgttcc
     3361 cgctggaatt ggggctgtgg atactccggc cggggaactt cctgggcaag cgaatatgcg
     3421 ataagggcga atagcagaaa tgcgcatcta atcatgcaat cagcatacaa gttccggggc
     3481 cgggagccgc tgcggaggcc aggaaaattt gcgcgaacac gtatcctccg cataacgtat
     3541 ttataagcag agagttagaa atgccattct gcacacaatg cggaaatgcc gtacgggata
     3601 cggacatcta ctgcggcacc tgcggcacca ggcagggcgc tgggccctcg ggaggcggga
     3661 gcgggagtca gatcgcgtcg ggactcagcc ctcgcaccgc cgcgctgctt tgctatgtcc
     3721 ccattgtcgg ttggatcgcg tccatcgtcg tactggccgc ggcaaagttc cgcacagacc
     3781 gggacgtccg gtttcatgcg ttccagggcc tatacctgtt tgttgcctgg ctgatcgtgg
     3841 actgggtcgt cgcgccgttc ttcactttcg tgccccgcca tatgctgccg gtcggctcca
     3901 ttctgaagct ggcgattata ggaacgtgga tcttcatgct ggtaaagacc gcacaggaac
     3961 agatgttccg gcttccgatt atcggcgagc tggccgaacg atcggtagcc gagcaaaagt
     4021 agagccccgc caccggtgca actgatttta gttgcactat cttttcagat atagcagact
     4081 gttgttgagg aacagatttg gttcccatat ggaccaacac cagaagctgc ttgatcgatt
     4141 acgaggacgg ccgaaagatt ttcgatggga gcaactcaca acactgctcg gtggatttgg
     4201 ctatgaagaa gagaaaggaa gcggttctcg ccgaaagttc ttcaatgcca agacgggcgt
     4261 cagtatcagc ttgcaccggc cgcaccccgg aaatgaacta aaggcttatc aggttaggga
     4321 cgtgttggat catttgaggc aggaggggtt actgtgaggg atttcatgga gtacaagggc
     4381 tacatcgggt cggtacacta cagtggggaa gatgaggttt tccacggcaa acttcaagga
     4441 attcgcgatc tgataacata cgagggcact gacgtcgggg gcctgaagca gagcttttac
     4501 gaggctgtcg atgattatct cgccgactgc aaaaagcggg gtaaggcacc cgaccgtccg
     4561 ttcaaaggaa cattcaacgt cagggttgga cgagaactgc acaagcgcgc tgccgtattc
     4621 gcttcagagc gaaaaaggaa actgaattcg gtagtcagtg aggcgctcga gaagtacctg
     4681 gaaaccgcgg attgcgggta gatatagcgc tcccgcacta aagcaaaaac gagaaacgca
     4741 acccggaacg ctgtcccctt ccgctcgtgt tatatttatt catatcggtg aataaatatt
     4801 cctcatgaag catacagtcg gcattgtcgg ttttcgcggc tatagcggaa tggagctgct
     4861 tcggatcctg gagcgccatc cgcacatcca tccggttctg ctggagcatc ggacggatgt
     4921 gggcgagcag agcaatctgc ggcgtaaagc cggaccgccg cggctcccct gtacggcgga
     4981 agcggcgaag gaacatgggt tagcggcggt cttgctcgcc acgccggcgg acgtatcgat
     5041 gggattggcg aggacgttcc tcgacgccgg catcaaggtg gtggacctca gcggcgcgtt
     5101 ccggctgcaa tcaccggaaa actatcaccg gtggtacaag gaagagcaca cggagccggc
     5161 actgctggcc gaagcagcgt acggattgcc cgaattctgc cggagctgca ttccgggagc
     5221 acggctagtg gcaaaccctg gctgttatcc gacagcggcc aatctcgcac tgaagccgct
     5281 ggttgcggcg ggcgtgatcg atcgcccggc aggcattgtg tgcgatgcca agtcaggggt
     5341 aagcggcgcg ggcagaaagc ccagccttaa gacaagcttc tgcgcggtga cggagaattt
     5401 ttccgcctat tcgattctgg agcaccggca cgttccggaa gtcctgatgt attcggggat
     5461 tgaagaggcg gagttcagct tcacggcgca actgttgccg ctggatcgcg gcattttgga
     5521 aacaatctat ttccggtcgg ccaaactgga gagcgccgcg caactgatcg atatttacga
     5581 ggcgcagtat ggggaggagc cgttcgtacg cttgtacgag gccggccgtg ttccggacct
     5641 tcggagcgtt cagcacacga actattgcga cattggcgtc aaattcgacg ggactaccgg
     5701 cagggcggtg gtggtggccg caatcgataa ccttgtgaag ggcgcggcgg gtcaggcgat
     5761 ccagaacctc aatctcatcc taaaacgtcc tgaaacggaa gggctgctgt gaggatcctg
     5821 atcaaactgg gtggcacgct gctcgacagc gacgactcgc gccggcggct cgcacgcgag
     5881 attgccgcac tgggccgcac tcattccgta gtggtagtgc acgggggcgg caagcagatg
     5941 acccgctttc ttgcggagcg cggggtggag agccgttttg tggagggttt gcgcgttaca
     6001 acaccggaag tgctggacgc agtgctcaaa gtgctcgccg gcaccgtgaa caagcaactc
     6061 gtttccgcgc tgatcgaggc cgggggcgat gcggtgggaa tcagcggcat cgatggttgc
     6121 ctgacggaag ccgagacgtt acggcccgaa ctcggggccg tggggcggcc gctgcggacc
     6181 aatccggcgg tgttaaacgc gctcagcggc gcaggcttca tcccggtggt ggcctgcgtg
     6241 gccggagatc gccagggccg gatgttcaac gtcaatgcag atcagatggc cgtgtcctgc
     6301 gcggcgggat tcggcgcgga gcggctgctg tttctcacgg acgtggaagg ggtgcgcggc
     6361 gccggtgggg cggtgttggg aagcctgacg gctggagatt gcatccgcct ggttcgcgac
     6421 ggcaccgcca ccggtggaat gcaggccaaa ctcaatgcgg cggtgcaggc actggagcaa
     6481 ggcgtgggcg aggtggtgat tctacccgga tatgagccgg aaattctgga tcgcgcgctc
     6541 cgcggcgagc ctctcggcac ccgcctgatt ccggaggaag tacgccggtg aacacgctgg
     6601 tcacattggc tccgccgctc gaacgggcag cacctccctt cgttgagaac gcacctgtcg
     6661 aaatccgaat ggcgaggatg agcgacgtgc gcgcgcttct ggatctcatc aacggctatg
     6721 ccgcggaagc gatcatgctg ccgcgcaccg aattcgagct ggcggaaaat attcgcgatt
     6781 tcacggttgc tgccgtgggt ggagacgtcg ttggatgtgc cgcgttgcac ttctatagtg
     6841 cggcgacggc tgaggtgcgt tcgctcgccg tcgctcccca atggaaaggg cacagcgtgg
     6901 ggcgactact ggtggagagt ctcgaaaaag aagcgcgcaa ttacggcctg gcctcgctgt
     6961 ttgcgtttac ttatgttccg gcttttttct cgaagctggg cttcgtcgag gtggaacgcg
     7021 gcgaattgcc gctgaaagca tggaaggatt gtttgcgttg cccgaaattt caatgctgcg
     7081 atgagatcgc gatgataaag aaactttgaa attccccttg gccgctcccc gctactgcgg
     7141 gtaaatccca ttactaaagt actgttcgtc cggtccaaaa taatcaatag tatccggaac
     7201 gtaggtgaat attcccctgc tttctcaggc ctttaacgaa caacggccat accccccaaa
     7261 gtgaaattga atatgccctc aaaataccca gcaaacttga gagaaaaagc atgggacaga
     7321 tactagtcat ctgtataatt agtgcagcaa acccagtttt cgctgcgttg aggtggtcgg
     7381 gtaatgagag ctcaaacggt cgatgtcagg gagtcttcgg gcaaggttct gtgttgcacg
     7441 atctttcgtg ccggcgggaa gaagctcctg gccaagggcc acgtaatcag cgaagaagat
     7501 atcagactgc tcgaaaccga agggatggat caggtctggg tcacggtttt agaggaaggc
     7561 gaagttgccg aggacgacgc agtctgcgct gtggcgcagg aaatgagttg cggatgttcc
     7621 gagatccgcc tggcggcggg cggacgcgcg aacctggtag cgacggaaga ctgctgcgtg
     7681 ctggtggatg acgaactgct gaagcagatc aattgcacag ccagtatcgt gatcgcgaca
     7741 gcgcccaact tcagccatgc aaaagcaggg cagcggatcg ccacggtgaa aagcgctcct
     7801 tttgctgttg cgcaatcaca gctagaggct gtaatctcta tattgaacga aagaggccct
     7861 atactccagg cgcgaccgat gaggacgccg aaggtcgccg tgttgtatac ggaccccgcc
     7921 agcggggatc ggtctcgaca gctctttgaa aacgtgatgc gccagcggtt ggagcggttt
     7981 ggtacgacgg ccagttttgt gctgagcgcg gtggaagatg aggagaccgt tgcaaaaagc
     8041 ctgcatcatc ttgcgcgagc gaagccagcg gtgattctgg tggcgtccac gactgcaccg
     8101 gccggaccgg aagacgtagt cggcagggct atgacgaagg tcggttgtca tctggagcgt
     8161 ttcatggctc cggtggaacc cgggaacctt ctgctgttag gatatttgga cgatctgccc
     8221 attgtttcgg ctccgggttg tttccggtcc gccaaactga atgtgatgga tttgattttg
     8281 cctccaatgc tggcgaagta tcgggtatca gggtgggaat tggcctgtct gggccacgga
     8341 gggcttctcg cctgaactat tagtctcctc cactaccacg ggccgtttcc tccgagcggt
     8401 ttgtgggtgc tcctcagccg ggcgcctgac cagcgtccgg ctttttttcg cagcctgacg
     8461 attcggcggc agcagcagca tgatgaaggc ggcgcacaat tgacaaacag cggtattggc
     8521 tgaatgctgg agaatggcgc ctgtatgaag gtcaccaaag tccagccctt tcttctttct
     8581 tatcctttcg atacaccggt gcggctttcc tatcacggag gtgaacgcac gatccacaag
     8641 cgcgatgcga tgctgattcg cattgagacc gaccaaggtt tggttggata tgcgccgggt
     8701 cagggcagcg aggcggcgca tgccgccatt caggattcca tcgctccctt tctggaaggc
     8761 cggaccctgg cggaccccga tgcgttgcgt gtactgttcc tgaaaggccc gggccggaac
     8821 ccgctgctca cacgggtcta ctgcgccgtg gagatcggtc tttacgatct gattggcaag
     8881 cagaaaggcc tgcccgtgtc ggagttattg ggcggcaggg ttcgggatcg tatccggctg
     8941 tacggcagtg ccggcatgta catgcccccg gaaaagtatg ccgaggaagc gctgacagtg
     9001 gccaatctcg gtttccgcgc ctacaaaatg cggcccgcgc tgggtgagca aaaggatctg
     9061 gaaaccgtgc gcctgatgcg ggaagcggtt ggctcggatt tcgatctgat ggtggatgcc
     9121 catacctggt ggcgaatggg cgaccgcagt tactcgttcg agaccgtcca gcggcttgcg
     9181 cgggacatgg ccgagtacga tatcgcctgg ctcgaggaac cgctgcctcc cgacgaccac
     9241 gaagcgtacc gcacgctgca tgacctcgac tatgttccac tcgcgagtgg ggaacatgag
     9301 ccggacgagc ccgggtttat ggatctcatt cagtccggcg gcgtcgatta cgtgcagatg
     9361 gatgtaattt gccagggggg ctacccgaca gggcggcgca ttttcgggga gattgcacgc
     9421 cagggcctcc gcttcgcctt ccatagttgg gggacggcgc tcgaagtgat tgcggcggcg
     9481 cacctgggaa tctgctggcc cgaaacagta gccgattggc tggagtatcc ctgttattcc
     9541 actccctcgc gggcaggcat gtatccgttc ccgctcgccg cggagattct gaaggagccg
     9601 ctggagattg atggtggtga cttgatcgtc ccgcgcaagc ccggcctcgg agtggaaata
     9661 gacgagtcga ttatcacacg ctacccgtgg attcccgggc cgtggtccta ttttcgctgg
     9721 attctcctgc cgggacatgg gcggtgacat cggaccacag cctgaaatgg acggaaggcg
     9781 ggccggctgc ttgaaggcga tctgttccac ggccattctg ggcgccggcg ccgcgctggc
     9841 ctggggggtt cgaggccgct cttctgcgct gtttgccccc tccgtctatc ggggacgccg
     9901 ggaccgcccg gccatcgcgc tcacgtttga cgatggaccg agtgaatcca caccggtatt
     9961 gctcgagcta ttggccaggt actccgtgcc ggccactttt tttcaatgcg ggttgaacgc
    10021 ggctcgcctg ccccgggtgg cgcgcgaggt ggcgctggcg gggcacgagg ttggaaatca
    10081 ttcccatacg catcgcctgt ttccgtgcct gacgccggcc gaggttcgcg aggacctggc
    10141 ccgagcgcag cgtgcgatcg aggatgccac gggcgtgact cctcgcttgt ttcgcgcgcc
    10201 gtatggggcg cgctggtttg gcttgagacg ggctcaaagg gaactgggat tgttaggcgt
    10261 catgtggact tctatcgcgc tcgattggaa gttgccttct gccgccatta cacgccggat
    10321 tcttcgagcg gcatccaatg gcgctatact gtgccttcat gatggtcgtg agttacgaat
    10381 cgatccagac atccgtaaca cggccacggc ccttcgcggc gccattcccg ctttagccgc
    10441 gcgcgggttt tgttttgaga cggtgagcaa catactatga acgatgagct aacccaacga
    10501 gtgatcaaaa tcatcgcggc cactcaacat attgaattgg aaaagatcac tcccgagagt
    10561 acgtttcagg agctgggtat cgactcgctg gatggcatta atatcctctt tgctctcgaa
    10621 aacgagttct ccatcaatat tcccgacgac gccggacagc ggatccgaac cgtacgcgag
    10681 atggtggatg gggtccggac gctgatatca gggggcgacc cggctcaagc gcaggcctga
    10741 tatgccgggg cgccgaattg ttatcaccgg tctcggcgcg atctgcgcgt tgggccgcaa
    10801 caaagatgag ttttgggact cgctggtgcg aggcgtgtgc ggaatccggc cgctcgagac
    10861 cgtcgacagg acgcagtttc gggtacagaa cggcgccgag gtgctgggct acgattccgc
    10921 ggagcacttc cccggcaagg aacgcgagca gcttgaccgt ttcgcgcagt ttgccgttac
    10981 caccgggcgc gaagcgatcg ccgactccgg tatcgaatgg caggatgggc agcgcgagcg
    11041 aacggcaatt gtcacgggtt cctgcgtcgg cgggcaggcc accgaaaatg agggttatgt
    11101 gaacctctac ctgctcaaga actctcgcgt gaacccgctc acgattcccc gcagcatgga
    11161 aaacgccggg gcgagcagac tttccctgga gtttggaatt acaggtcccg tgtacacggt
    11221 ttctaccgcg tgttcctcct ccaatcacgc cattggccag gcgttttggc tgttgcggca
    11281 aggcgtctgc gatatagccc ttgccggcgg cagtgaagct cccttcggca cggggatgct
    11341 gaaggcgtgg gaagcgatgc gtgtactcgc tccggatacg tgccggcctt tttcgaaaga
    11401 ccggcgcggg ctggttctag gggagggcgg cgccatgctg gtgatggaga ccctggaggc
    11461 ggcacgcgcg cggggcgccc gcatctacgc cgagatattg ggattcggaa tgtcatccga
    11521 cgcacaccat cttacgcagc ccgccgccga gggggctgcg cgtgcgatgc gaagcgcgct
    11581 ggccgacggc tgcctcaatc catcgcaggt cggctatatt aacgcgcatg ggacgggcac
    11641 gctcgcgaac gatcctacgg aggtggcggc gatccggtcc gtgtttaact ctcacgccag
    11701 cgatatggca gtcagttcga cgaaatcgat gcacggccac gctttggggg ccgcgggagc
    11761 gattgaggcg gtggcaacta ttctggcgct ggatcgcggc ctactgcctc cgacggctaa
    11821 ttttaccgaa cgcgatccgc aatgcgatat cgacgtaatt ccgaacaccg cgcgggagca
    11881 aaaagtagat tacgccttgt cgaactcctt cgcattcggc ggattgaatg ccgtgctcgt
    11941 ctttggcagg ctccggggct gaacctcaaa agctgaaacc gaattcgccg aagacattca
    12001 cccagcggtc tcttgtgcgc agagccgaca attctccgac cggatctccc tcggtatgcc
    12061 cgcgatagac gcgtgtcacc gcgccgatcc gaaaatgccg gccctggtcg aggaaccagt
    12121 tcactccaaa caggccgtag tagccccagc cgctgcggga agtgcaaacg ggacagtcaa
    12181 actggtagta atcgctcggt tggcgaatac gctccgaata ccgcaggtaa gcgcctccgg
    12241 ctccgccgga tatcagcagc ctgccgtcgg caagcggtgc gatgacgcgg cccccgaagg
    12301 gcacgaaata ttggaaatcg cggatccgca aatacccgaa gccgctatcc aggaactctc
    12361 tgacccccgc ggctccaaac gccgtatcca ggcccacgtc tgcctgaaga taccggtgga
    12421 agcgataacc atatccggcg cccaaaacaa aactggtatc gtagaacggt ttcagatctc
    12481 cccttggctg tgctccaccg aggcccagcg gtatattgtg tttcggataa tcggattggg
    12541 cggccaggac agcggccgta attaaaagca gcgcaagggc tttcacatca agtcagacgc
    12601 ccgtagcagg aggggcgtta cagtggtcgc tcgttttgta ccgccggatg ctcctgacgg
    12661 gttacgccgg cagcccgcct gcgcggatc
//
LOCUS       DASTMK010000303         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_270910, whole genome shotgun sequence.
ACCESSION   DASTMK010000303 DASTMK010000000
VERSION     DASTMK010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270910"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFQ79_13265"
     CDS             complement(<1..397)
                     /locus_tag="VFQ79_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU0140684.1"
                     /translation="MNGGEFRRKAKRFADRKGLAYRWVPARGVGSHGTVYIGSRKTFA
                     YPAWFRPDEDRRPVVSFPDFPHAHTDGRDMRDAMEEAIDCLGSVVAQYMAEKAEIPFP
                     SAPKRGQKLVPVPLWIAGKLGLYLAMCEKG"
     gene            536..1201
                     /locus_tag="VFQ79_13270"
     CDS             536..1201
                     /locus_tag="VFQ79_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140685.1"
                     /translation="MPHRLFPAVALACCSCLLGADDKRPEGRYINEAIEVSAKLYHDK
                     NSVRELLGSDFDGTIVVVDVAVSPKGDSPLKVYRDDFTLLSEKDGQKSQPFAPSQIAG
                     EGALVISTTGRGGPAMMSEDRGPIWGGTMDRPRRMGGEGAAVGNTGSTEVQATVQTDK
                     GSKANPMLALLKEKVLQEGEIGEPVQGLLYFPMEGKHKTKQLEMMYKGPAGKFSIRFK
                     EPK"
     gene            1208..2299
                     /locus_tag="VFQ79_13275"
     CDS             1208..2299
                     /locus_tag="VFQ79_13275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0140686.1"
                     /translation="MHISELDTPALAIDLDIMERNLTRVADYTRAHGLRLRPHTKTHK
                     IPALGKRQVELGAAGLTVAKVGEAEVMLASGTPDLLIAYPVIGRLKLERLVKVARETR
                     VTVSLDSLYAAQELSHAAQSGGVEFGVLAEVDVGLGRVGVPPGEVLSLVDSLRRLPKL
                     TFEGIAFYPGHIKEMNNGGGKQLSALNEAVRGMLSCLEKAGVKANVVSGGSTPSLYHS
                     HLVEGLNEIRPGTYIFNDRNTVATGACGWQDCAASILVTVVSTAVKDQMIIDGGSKTF
                     SSDGLITGADPGFGRVAEAPDAAFVRMNEEHGYVDMHNSSVRFEIGQRVRVIPNHICA
                     AMNLHETVYGIRGDQVETTWKVEGRGKLQ"
     gene            complement(2315..>2878)
                     /locus_tag="VFQ79_13280"
     CDS             complement(2315..>2878)
                     /locus_tag="VFQ79_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU0140687.1"
                     /translation="ALWEFLEETTSYLSIIELGLYESSAKTYSALAERGLEPGTAEWK
                     QAVEEVLDRQRNAMAPRLWPEIPPARYLCFYPMDRKRGETINWYQLPMAERQRQMHEH
                     GMIGRRYAGEVRQIISGSIGFDDWEWGVDLFADEPLVFKKLIYEMRFDQVSAVYAAFG
                     SFYVGVRVPASGLGELLDGSLPEMAAR"
BASE COUNT          635 a          865 c          841 g          537 t
ORIGIN      
        1 tgcccttttc gcacatcgcg agatagaggc ctaatttgcc agcgatccag agcggtaccg
       61 ggaccaactt ctggccgcgt ttcggcgcag aaggaaaggg gatttccgcc ttctcggcca
      121 tgtactgagc gacgacactc cccaggcagt cgatagcctc ctccatcgcg tcacgcatat
      181 cccggccgtc tgtatgggcg tgcggaaaat cgggaaaact cacaacaggc cgtcgatctt
      241 cgtccggccg aaaccatgcc ggataggcaa acgtctttct gctgccgata taaaccgtgc
      301 catgactgcc tacgccccgc gcaggaaccc agcggtaagc gagtcctttc cgatccgcga
      361 aacgcttcgc ttttctccgg aactcgccgc cgttcatcca gttctattct cgcacacaaa
      421 tgtgcgaaga attccacgcg cggagagcgg ctccggtaga tcggcggcgt ccttcggaat
      481 ttcatcgtgg aggatttatt ccaggacaag cgtataatcg gcaaggggga tcaccatgcc
      541 gcatcgcctg tttccagccg tagcgctcgc ctgttgctcc tgcctgctcg gtgccgatga
      601 taaacggccg gagggccggt atatcaacga agccatcgag gtatcggcga aactttacca
      661 cgataagaat tcggttcgcg aactgctcgg gtcggatttc gacggaacca ttgtggtggt
      721 ggatgtagcc gtctccccca aaggagacag tcccctgaaa gtttatcgcg acgatttcac
      781 tctgctgtcg gagaaagacg gccagaagtc gcagccgttc gctcccagcc agatcgcggg
      841 agaaggcgcc ctcgtgatat cgaccacggg acgcggaggc cccgcgatga tgtcagaaga
      901 ccggggaccg atctggggcg gcaccatgga tcgcccgcgc cggatgggcg gcgaaggagc
      961 cgccgtcggc aacaccggaa gcaccgaagt gcaggcgaca gttcaaacgg ataagggaag
     1021 caaagcaaac ccgatgttgg ccctactaaa agaaaaagtt ctgcaagagg gagagattgg
     1081 agaacccgtc caaggactac tctatttccc gatggaaggc aagcacaaaa ccaagcagct
     1141 cgagatgatg tataaaggtc cggcaggcaa gttttccatc cggttcaaag agcccaaata
     1201 atagatcatg cacatttccg aactcgacac acccgcgctg gccatcgacc tcgacatcat
     1261 ggagcggaac ctcacccgcg tggcggacta tacacgcgct cacggcttgc gtctccggcc
     1321 gcacaccaag acacataaaa tcccggccct ggggaaacgc caggtggaac tcggagccgc
     1381 cgggctcacc gtcgccaaag tcggggaagc cgaagtgatg ctcgcgagcg gcacacccga
     1441 cctgttgatc gcgtatccgg taatcggacg cctgaagctg gagcgtcttg tcaaagtggc
     1501 tcgggagact cgcgtgacgg tttcgctgga cagcttatac gccgctcaag agctctccca
     1561 tgcggcgcaa tccggcgggg tcgaattcgg tgtactggcc gaagtggacg tgggattggg
     1621 ccgcgtcggc gttccgccgg gtgaagtttt atcgctggtg gacagcctcc gccggctgcc
     1681 gaagttgaca tttgaaggaa tcgctttcta cccggggcac atcaaggaga tgaacaacgg
     1741 cggcggcaaa cagctcagcg ctctcaacga agccgtacgg gggatgctgt cgtgcctgga
     1801 gaaggcaggc gtgaaagcga acgtcgtgag cggcggatct acaccctctc tataccactc
     1861 acacctggtg gaagggctga acgagatccg gcccggcacc tacattttca acgaccggaa
     1921 cacggtggca actggtgcgt gcggctggca ggattgcgcg gcgtccatcc tcgtcacggt
     1981 tgtttccact gccgtgaagg accagatgat tatcgatggc ggctcgaaga cgttttcttc
     2041 ggacggcctg atcaccggcg ccgatcccgg cttcgggcgc gttgcggaag ctccagacgc
     2101 cgcgttcgtc cgaatgaacg aggagcacgg ctacgtggac atgcacaact ccagtgtgcg
     2161 attcgagatc ggacagcgcg tccgcgtgat accgaaccat atctgtgccg ccatgaacct
     2221 gcatgagaca gtctacggga ttcgcggtga tcaggtggaa accacctgga aagtggaggg
     2281 ccgcggcaag cttcaatagc gaagcacgtg aatcttagcg cgccgccatc tccggcagcg
     2341 acccgtccag caattctccc aacccggacg ccggcacacg cacaccgaca tagaacgagc
     2401 cgaaagccgc gtagaccgca ctcacctggt caaagcgcat ttcgtagatc agtttcttga
     2461 ataccagtgg ttcgtctgca aacaggtcta ctccccattc ccaatcgtcg aacccgatgg
     2521 aaccggaaat gatctggcgc acctcacccg catagcgccg tccgatcatg ccatgctcgt
     2581 gcatttgccg ctgccgttcg gccatgggca actggtacca gttgatcgtt tcgccgcgct
     2641 tgcggtccat cggatagaag cagaggtagc gtgcgggtgg aatttccggc cacagccgcg
     2701 gtgccatcgc attgcgctgc ctgtcaagaa cctcctccac cgcctgcttc cattcggcgg
     2761 taccgggttc gagaccgcgc tccgccagcg cggaataggt tttcgccgac gattcgtaca
     2821 gccccaattc aataatggac aggtacgatg tggtctcttc gagaaattcc cagagcgc
//
LOCUS       DASTMK010000304         8475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_590934, whole genome shotgun sequence.
ACCESSION   DASTMK010000304 DASTMK010000000
VERSION     DASTMK010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590934"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFQ79_13285"
     CDS             complement(<1..1383)
                     /locus_tag="VFQ79_13285"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140688.1"
                     /translation="MSSVPRFALLCLAFVLAAPASERDVAEWVIRLGGRVIVEGNRTP
                     LRDVSELPQSELRIRGLDFTNTTLDPGDLEKISHLSGLRELYLPGPSWNPGAGSRLDA
                     NDQLRFLSGLTNLKRLYFSLHFLANVNVRDKGFAHLAGLTQLEELRCSQCRITADGLA
                     PFTNLRALDLNYATIGDEGIKSLRGMKELRRLLLRDTLITDQGLQSLSGLVKLEELDL
                     YGVNITERGLESLRPLTGMRKLNLLGAGITDEGVEVLAGMKHLRELNLYRSRITNAGL
                     AKLAGLKDLVALDVRYSRVTQNGVEAFQAGVPDCKVDFVGTAVARVREPGAGQPSDDD
                     PRAVAEWVKALGGTAEFAGGALRSISLASTGVSDAQLAYLGGIRTLKKLDLTATETGD
                     LGLSALKNLTGLKELILSHTTVSDAGLEQLAGLRQLQILRLGGTLVRGSGLRHLKELT
                     ALTELDLTSAP"
     gene            complement(1414..2727)
                     /locus_tag="VFQ79_13290"
     CDS             complement(1414..2727)
                     /locus_tag="VFQ79_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140689.1"
                     /translation="MQRRNFMEGGITALSARRIFGANDRIRAGLIGCGARGRYVAGLM
                     AHSPGVEFTAVCDVYAANAAAALQWAGTGARAYADFRKVLDDKDIDVVLIATPDHWHA
                     IPAILACEAGKDVYVEKPLAHNVREGRAMVAAARRYNRIVQTGTQQRSAPHFAEVARI
                     IQSGELGEVRFVRVWNFSNMTPDGIGREADSEVPEGLDWEMYLGPAPRVPFNRKRFLG
                     TFRWFWDYAGGTITDFGTHRFDTVHQIMGVDAPEKVSASGGRFTLRDAGEMPDVLQVT
                     YDYPGFVLSYEASNLSAHGLGGRTAGMRYYNARGAEDRPNGMAFYGTNGALFADRIGY
                     EIYPDFRRPGNEPRIPARRMNTTDATELHARNFVEDVRSRRRPAADVETGHRSTLIGH
                     LGNIAFKTGRKLKWDAGRESFVQDTEADRLLGRSARKPWDLIPVI"
     gene            2881..5040
                     /locus_tag="VFQ79_13295"
     CDS             2881..5040
                     /locus_tag="VFQ79_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU0140690.1"
                     /translation="MKRIRRLLLWSFAFSVSILSAQNTALVCTASAVTTNVRAEGLTE
                     RLGDIILDCAGGAPGSGVTGNLSVILPVAVTNRINEESGAPDVTLTIDTGATPLTVSA
                     TVSRPNRVNFNGLNFSVGPTGRALLRISGIRIAAVQLGFTSGMAVPVPIVAIITMTGP
                     QNVPLVNSNPAVGIASSGLLASASSTAIVCIGSPLPDTVSFSALIGAGTHSYSTRVTE
                     GFPAAFEVRQNGARTGTRFIARYNGLPQGARIFVPDAIAGSSAIQPTARGDFGPPASG
                     GRYKRTAEGSLLLALVRGADATGAGGLPVYTPGPVGSEEVAFNGVTEIGLINGAGYAV
                     YEVMDANAAVRESAQWPTFVGMPRPDGGQSVIARQEITFAPVSELASASTSAPVPRFA
                     AVAPPPDCPALNDCKSSYMPRLAVDAPLLEFTAAAGSIYKVNYIGVENEGGGLLVWAA
                     TLAYKNGSDWIRLEQPSGLNAGVIRLDVLPEKLAPGRYEATLTIDAGPLAGTRSLPIR
                     LDVTPGPAPIAPVPVIRTVQNAASLAPGPLVAGSLATIMGEKLGGEQVTVRFDGIPAK
                     LLYSSDTQINLQVPEAIGAMAEARVVVSADGVEGIPVLVTLRPAAPAIFQSGILNQDS
                     SANTESAPAATGSVIQIFGTGLPTQGTITAKIHDREIADPIYAGAAPGLDGVQQVNIR
                     VPDDLPAMTTEVLLCATAPGMERVCSAPAPVTLQRTQ"
     gene            5080..6522
                     /locus_tag="VFQ79_13300"
     CDS             5080..6522
                     /locus_tag="VFQ79_13300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0140691.1"
                     /translation="MLSVLVPLYNEEEFVGALLERVLAAPLPRQMAKEIIVVDDGSND
                     GSAEVVERFAAQHSGCIKLVRHETNRGKGAAVRTALEHARGEFCVIQDADLEYDPRDY
                     PRLLHPLLEGKADAVYGSRFLVAGERRVLYFWHALANNLLTTFCNIAADLNLTDIETC
                     YKAFRTSLLKSIPLRSDRFGIEPELTIKLARRQARIYETPINYHGRTYEEGKKISFAD
                     AVAAFFVIARYAFTSDLYNDPGQKILDSFACASRFNRWMADVISPYLGRNILEIGAGI
                     GNLTRHLISRRERYIAADIDREHLARLRARLHHRPNLQTLPCDLNRPDDFRELAGSVD
                     TVVCLNVLEHVDDELCALANIRTALCDGGRAVILVPEGPGIYNELDAALGHSHRYTEE
                     HLRKCLRSSGFRVERMIPFNRISRPGWYLNGSVLRRRTISPGQLRTFDRLVWLWKRID
                     NYLPWPPTSLIAVAVKENQAIERPAISAGN"
     gene            complement(6503..>8475)
                     /locus_tag="VFQ79_13305"
     CDS             complement(6503..>8475)
                     /locus_tag="VFQ79_13305"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEU0140692.1"
                     /translation="RALITGSVIGVVLFLIGLFALSQFRDKAPEPASTGPARVSVVSV
                     PKGAAIRVDGELRCVADCSFDLLPGTHEIEARLEGYEPLKRIIDARAGEPVSLNLPLS
                     LEAQSVRIAADMLSGEVLLDGNSATQLQEGQAVLENVQPGSHTIRIAGGSASAEFTLD
                     VNPGQAPLLSGPISTRNVLAVLVSNKGDQARLYTSSNPWKVYMGGQQVGETVPAGIDL
                     TPPSPGDHELTIGEGSQQRKLIVNFGPAPVLAVFLKTDVNAGTLVVATGEDDVQVFLN
                     GRPHRRATARGQLRIPGLPVREYAVRVEKEGYQVSPGEQRAMVRKGEETRLEFHLQPL
                     PQVASLRISGAAAGAQVFLERERIGSVRSDGTFAHEGIAPGKHTIEIRQQGYVPRQFA
                     REFRAGETVELRGSELAMAKQPATLRLVLSPPDAKVTIRGAEETQARTLSQPSAELPE
                     GSYSITATAPGHNSQSVTVQLSAGQSRTVELRLRKQEVPPAPKVLTLADWPGVWEKRE
                     EWFVRKGGDFVLFPVTPTSGTFLFTATMLKSTAVFRQRHLEWVVAFTDPKNYLAFEID
                     NKNLSRKEVRNGRSRDLPKVPHGLSPDQKVYTISIEISPGHVTHRIRRGDGWAVLDKI
                     ADDSLDFERGKFGFRIQGNDEVGVRNFSFQPK"
BASE COUNT         1626 a         2681 c         2493 g         1675 t
ORIGIN      
        1 aggagcactc gtaaggtcga gttccgtcaa cgcggttaac tccttcaggt gacgaagtcc
       61 cgatcctcga acgagcgtgc cgccgagtct caagatctga agctgacgca gacccgcgag
      121 ctgttcgaga ccggcatccg atacggtcgt gtggctgaga atcagctctt tgaggcccgt
      181 caagttcttt agcgctgaca gacccaggtc gccggtttcg gtcgcagtga gatccagttt
      241 cttcagtgtt cggatgccgc cgaggtatgc gagttgcgcg tcacttacgc cggtggaagc
      301 gagcgagatc gagcgaagcg cgccgccggc gaactcggcg gtgccgccca gagccttcac
      361 ccattcagct acggcgcgcg ggtcatcgtc ggatggctgt cccgcgcccg gctcacgcac
      421 acgtgctacc gcggtgccca caaagtccac tttgcaatcg ggcacgccgg cctggaacgc
      481 ttccacgcca ttctgtgtca cgcggctgta ccgaacgtcc aacgccacca ggtcctttag
      541 tccggcgagc ttcgccagtc cggcattcgt gatacggctg cgatacaggt tcaattcgcg
      601 cagatgcttc atgccggcga gtacttccac accctcgtcg gtgatgccgg ctccgaggag
      661 attcaacttc ctcataccgg taagcggacg cagcgattcc agaccgcgct cggtgatatt
      721 caccccgtaa agatccaact cctcaagctt cacgagaccc gataacgact ggagcccctg
      781 gtcggtgatg agcgtatccc gcaataacag gcggcgaagt tctttcatcc ccctcaggct
      841 cttgatgcct tcgtcgccaa tcgtcgcgta gttcagatcc agcgcccgca ggtttgtaaa
      901 cggcgccaga ccatccgcgg tgatgcggca ttgggagcag cgcagttctt cgagttgcgt
      961 caaccccgcc aggtgggcaa atcccttgtc ccgcacgttc acgttcgcaa gaaagtggag
     1021 actgaaatac agccgcttca gattggtcaa acccgagaga aaacgcaatt ggtcgttcgc
     1081 gtccaatcgg ctgccggcac caggattcca gctcggaccc ggcaaataca actcgcgaag
     1141 cccggacaga tggctgattt tttcgagatc tccggggtcg agcgtcgtgt tcgtgaagtc
     1201 gaggccgcgt attcgcaact cactctgcgg caattcggaa acatcgcgca gaggcgtacg
     1261 attgccttcg acaatcaccc tgccacccag ccggatcacc cattccgcaa cgtcgcgttc
     1321 cgatgcgggc gccgccagta caaaggccag gcaaagtaac gcgaatcgtg gcacgctcga
     1381 cattcccagt tccgctccca gctcttcccg ccactatatc actggaatga gatcccaagg
     1441 cttgcgggcg gatcggccga gcagccggtc ggcctctgtg tcctgaacaa aggactcccg
     1501 cccggcatcc catttaagct tccttcccgt cttgaatgcg atgtttccca ggtgaccgat
     1561 gagagtggac cggtgcccgg tctccacatc cgccgccggc ctgcggcggc tccgcacatc
     1621 ttccacaaaa ttcctggcat gcagttcggt ggcatccgtc gtattcatgc ggcgcgcagg
     1681 gatacgtggt tcattgccgg gacgcctgaa atccgggtag atctcatatc cgatccggtc
     1741 ggcaaacagc gcgccattgg taccgtagaa tgccatgccg ttgggccggt cctcggctcc
     1801 tcgcgcattg tagtagcgca tccccgcagt cctaccaccg agcccgtgcg cgctgaggtt
     1861 cgacgcctcg taactcagga caaagcccgg gtagtcgtac gtgacttgaa gaacgtccgg
     1921 catctctcct gcatccctca gcgtgaacct cccgccggaa gcagacactt tttccggcgc
     1981 gtcgaccccc atgatctggt gtaccgtgtc gaatcgatgg gtaccgaaat cggtgatcgt
     2041 tcctccggcg taatcccaaa accagcggaa cgtgccgaga aaccgctttc ggttgaaagg
     2101 cacccgtgga gcgggcccca ggtacatctc ccaatcgaga ccctcgggga cttccgagtc
     2161 ggcctcacgg ccgattccgt cgggcgtcat gttgctgaaa ttccatacgc gcacaaagcg
     2221 gacttcgccg agttccccgc tctggatgat tcgcgcgacc tccgcgaaat gcggcgcgga
     2281 ccgctgttgg gtgccggtct gcacgatgcg gttgtaacgg cgggccgccg ctaccatcgc
     2341 gcgcccttcg cgcacattat gagccagcgg cttttcgacg taaacgtcct tgccggcttc
     2401 acacgcgagg attgccggaa tggcatgcca gtgatccgga gtggcgatca gcactacgtc
     2461 gatgtctttg tcatcgagca ctttccgaaa atcggcatag gcccgcgcac cggtgccggc
     2521 ccactgtaag gcagccgcgg cattcgcggc gtagacgtcg cacacagcgg taaattccac
     2581 gcccggcgag tgtgccatga gcccggccac gtaccggccg cgcgccccgc agccgatgag
     2641 cccggcgcga atccggtcgt tcgctccaaa gatccgcctc gcggaaagag cagtgatccc
     2701 accttccatg aagttccgcc gttgcaccgc tcacctccac ccggacatcg tatctcgatt
     2761 ggtgtgtaat aatgtcttcg gatcgttcag gctcgtctcg cgctttcttc aatccaaacc
     2821 gcgtccacca ggcccggcta ccggtgtgca cgcccggcaa agggcttcag gggtgtcgaa
     2881 atgaaaagaa ttcgtcgatt gttgttatgg agttttgcgt tctctgtttc gatcttgagc
     2941 gcccagaata ccgcgctcgt atgcacggca tcggcggtca ctacaaacgt gcgggcagaa
     3001 ggcctgacag agagactggg cgacattatt ctcgattgcg ccggcggcgc tcccggcagc
     3061 ggcgttacag gaaatctttc ggtgatcctg cccgttgcgg taaccaatcg catcaacgaa
     3121 gagagcggcg cccctgatgt cactcttacg atagacacgg gcgcaacacc gctaacggtg
     3181 tccgcgacgg tttcgcggcc gaaccgggtg aacttcaatg gtttgaattt ttccgttggc
     3241 cccacagggc gggcactttt gcgtatttcg ggaatccgga ttgccgccgt acagctcgga
     3301 ttcaccagcg gcatggcggt gcccgtcccc attgtcgcga tcatcacgat gaccggtccg
     3361 cagaacgtgc ccctggtgaa cagtaacccg gccgtgggca ttgcatccag cggactgctg
     3421 gcaagcgctt ccagcactgc gattgtttgc atcggctctc ccctgcccga taccgtgagc
     3481 ttttccgcat tgataggtgc cggcacgcac tcctattcca cgcgggtgac cgagggtttc
     3541 ccggcggcct tcgaagtccg gcaaaacggc gcgcggacgg gcacccgctt catcgcccgg
     3601 tacaacggcc tgccgcaagg ggcgaggatt tttgtcccgg acgcgattgc gggctcgtcc
     3661 gcgatacagc cgactgcgcg gggcgacttc gggcctccgg catccggcgg acgatacaaa
     3721 cggacagccg aaggctcact gctgctggcg ctggtccgcg gcgccgacgc caccggcgcc
     3781 ggtggacttc cggtgtacac accgggcccc gtcgggtccg aggaagtggc cttcaatggt
     3841 gtcaccgaga tcggcctgat caacggcgcc ggttatgcgg tctacgaggt gatggacgcc
     3901 aacgccgccg tccgtgaaag cgcgcaatgg ccaacgttcg tcggaatgcc gcgcccggat
     3961 ggcgggcaaa gtgttatcgc caggcaggag ataacgtttg caccggtatc ggagctcgcc
     4021 tcggcgtcca cctccgcgcc cgtgccgcgg tttgcggccg tagcgccacc gcccgattgc
     4081 ccggcgctca acgattgcaa atcttcttac atgccaaggt tggccgtgga cgcgccgttg
     4141 cttgaattca ccgctgccgc cggctcgatc tacaaggtca actacatcgg agtagagaac
     4201 gaaggaggcg gactgctggt ttgggcggca acgctcgcgt acaaaaacgg ttcagactgg
     4261 attcgcctcg agcagccgtc cggactgaat gccggcgtga tccgtctgga cgtcctgcct
     4321 gaaaagctcg caccgggccg ttacgaagca accctgacga tcgatgccgg accgcttgca
     4381 ggcacgcgct cactcccaat caggctggac gtgacgcccg gcccggcccc cattgcgccg
     4441 gtacccgtaa tccggacggt tcaaaatgcg gcgagcctgg cgcccggtcc actcgtagcc
     4501 ggttctctgg ccacaatcat gggcgaaaag ctcgggggcg aacaagtgac ggtgcgcttt
     4561 gacggcatac ccgccaagct gctttacagc agtgacacgc agatcaatct gcaagtgccc
     4621 gaagccatcg gcgcgatggc cgaagcgcgg gttgtcgttt cggccgatgg cgtggaagga
     4681 attcctgtgc tcgtgacgct tcggccggcg gcaccggcta ttttccagtc cggcattctc
     4741 aatcaggatt cctcagcgaa tacggagagt gcccctgccg ctacgggcag tgtgatacag
     4801 atctttggta ccggcttacc cacgcaggga acgattacgg caaagattca cgaccgtgaa
     4861 attgccgatc ccatttatgc gggcgctgct cccggactgg atggcgtgca acaggtaaac
     4921 atccgtgtgc cggacgatct gcccgcgatg acgacggagg tcctgctttg cgcgaccgcc
     4981 cctggcatgg agcgggtgtg cagcgccccg gcaccggtga cgttacagcg aacgcagtag
     5041 caagaccgcc cgtgttgttg agcgataata accgggttca tgctttccgt actcgttccg
     5101 ctctacaacg aagaggagtt tgttggcgcg ctgctcgaaa gggtgcttgc cgctcctctg
     5161 ccgcgccaga tggcgaagga aatcatcgtt gtcgacgatg gctcgaacga cggttccgcc
     5221 gaagtcgtgg agcgattcgc cgctcagcac tccggctgca taaagctggt ccggcacgag
     5281 accaaccgcg gaaagggcgc ggccgtacga acagcgctcg aacatgcgcg cggggagttt
     5341 tgcgtcatac aggatgcgga tctcgaatac gatccacggg attacccacg gctgcttcat
     5401 cccctgctgg aaggcaaggc ggacgccgtc tacggttccc gtttcctggt tgccggagag
     5461 cgccgggtcc tgtatttctg gcatgctttg gcgaacaatc tcctgaccac gttttgcaat
     5521 attgcagccg acctgaacct caccgatatt gagacctgct acaaggcgtt tcgaacgtcg
     5581 ctgctcaaga gtattccctt acgcagcgac cggttcggga tagaacccga gctgaccata
     5641 aagctggccc gccggcaggc caggatctac gagacaccca tcaattacca cgggcggacc
     5701 tacgaggaag gtaagaagat atctttcgcc gacgccgtgg ctgcgttctt cgttatcgcg
     5761 cgctacgcct tcaccagcga tctctacaac gaccccggcc agaagatcct cgattcgttc
     5821 gcctgcgcgt cgcgattcaa caggtggatg gcggatgtga tctcccctta cctgggccgg
     5881 aacatactcg agatcggagc aggcatcggg aatctgacac gtcatctgat ctcccggcgc
     5941 gaacgttata tcgccgccga catcgaccgg gagcatctcg cgcggttgcg cgcgcgcctc
     6001 catcaccgcc cgaacctgca gaccctgccg tgcgatctaa accgccctga cgattttcga
     6061 gagctcgccg gaagtgtcga caccgtggtg tgtctgaacg tgctggaaca cgtggacgat
     6121 gaactctgcg cactggcgaa catacgcacg gcgctctgcg acggaggacg cgcagtcatt
     6181 ctggtgcccg agggcccggg aatttacaac gagctcgatg cggcactcgg ccactcgcac
     6241 cgctatacgg aagaacacct ccgcaagtgc ttgcgaagtt ccggcttccg ggtggagcga
     6301 atgattccgt tcaaccggat ctcacgtccg ggctggtatc tgaacggatc ggtattgcgc
     6361 cgcaggacca tcagccccgg ccaactacgg acattcgacc gtctcgtgtg gctttggaag
     6421 cgcatcgaca actatctacc gtggcctcct acctcactga tcgctgtagc agtaaaagaa
     6481 aatcaggcta ttgagaggcc cgctatttcg gctggaaact gaagttcctg acgcccacct
     6541 cgtcattgcc ttggatacga aacccgaact ttccccgttc gaaatccagg ctgtcgtctg
     6601 caattttatc gagcacggcc caaccatctc cccttctgat acggtgcgtg acgtgccccg
     6661 gtgaaatctc gatggaaatg gtatagacct tctggtccgg actcaacccg tgcggcactt
     6721 tcggcaagtc gcggctgcgg ccgttgcgaa cttccttgcg tgacaggttc ttgttatcga
     6781 tctcgaatgc aaggtagttt ttcgggtccg taaacgcgac cacccactcc aggtgccgct
     6841 ggcgaaacac cgcggtagac ttcagcatgg ttgccgtgaa caggaaggtt ccggaagtcg
     6901 gggtaaccgg aaacagaacg aagtcgccac ccttccggac gaaccattcc tctctcttct
     6961 cccacacgcc cggccagtcg gcgagagtca atactttggg tgcaggaggc acttcctgtt
     7021 tacgcagacg tagctccacg gttctgctct gcccggcgga aagctggacg gtgacggatt
     7081 gcgagttatg gccgggtgct gttgcggtga tggagtagga accctccggc aactcggccg
     7141 atggctggct cagcgtacgc gcctgcgtct cctccgcgcc ccgaatcgtc accttggcat
     7201 ccggcgggga aagaaccagg cgcagtgttg ccggctgttt agccatagcc agctcggagc
     7261 cgcgaagctc aacggtttcg ccggcacgga actcgcgggc gaactgcctg ggcacgtagc
     7321 cttgctgccg gatctcaatg gtgtgttttc ccggtgcgat tccctcgtgt gcaaacgtgc
     7381 cgtcggaccg taccgaaccg atgcgctcgc gttccaggaa tacttgcgct ccggctgcgg
     7441 ccccgctgat ccggagagaa gcaacctgcg gcagaggctg caaatggaac tccaggcggg
     7501 tctcttcgcc tttgcgtacc atggcgcgct gctcaccggg ggatacctga tatccttcct
     7561 tttcgacgcg cacggcatat tcccgcacag ggagcccggg aattcgcaac tgcccgcggg
     7621 cggtggcgcg gcgatgcggc ctgccgttca gaaagacctg cacatcgtct tctccggtag
     7681 ccactaccag cgtaccggca ttgacatcgg tcttcaggaa aaccgccagt acgggagcgg
     7741 ggccgaagtt tacgatcagt ttgcgctgtt gacttccttc tccgatagtc aattcgtgat
     7801 cgcctggcga aggcggcgtc aggtcgattc ccgccggcac cgtttcgccc acctgctgcc
     7861 cgcccatgta gaccttccag ggattcgagc tggtataaag ccgcgcctgg tcacctttgt
     7921 ttgaaacaag gacggccaga acgtttcgcg tgctgatggg accggatagc aacggagcct
     7981 ggccggggtt cacgtcgaga gtgaactcgg cgctcgcgga gccgccggcg atgcggatcg
     8041 tgtgactgcc gggctggacg ttttccagca ccgcctggcc ttcctgtagc tgcgtggcgg
     8101 aatttccatc cagcaacact tcaccggaga gcatatctgc cgcaatccgg acggactgag
     8161 cttccagact aagcggcaag ttcaggctga ccggctcacc agcgcgcgcg tcgattattc
     8221 tcttgagagg ctcatatcct tccagccgcg cttcaatctc gtgggtgccg ggaagcaggt
     8281 cgaaactgca atcggccacg caccgaagtt cgccgtccac acggatagca gcccccttgg
     8341 gaacactgac aacgctgacg cgggcaggac ctgtagaagc cggttccggc gccttatccc
     8401 ggaactggct cagggcgaac aggccgatca ggaacaacac gactccgatt acgcttccgg
     8461 tgatcagcgc ccgtt
//
LOCUS       DASTMK010000305        26240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_47022, whole genome shotgun sequence.
ACCESSION   DASTMK010000305 DASTMK010000000
VERSION     DASTMK010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 26240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26240
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47022"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFQ79_13310"
     CDS             <1..249
                     /locus_tag="VFQ79_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140693.1"
                     /translation="LETGGPAAGWYLKIKPDSSAGDAHLLRGAEVQPWVMRELERLQL
                     AFAPTASLADGGVLSEDLSKNVPRDEWELACGEMFLNP"
     gene            complement(333..2096)
                     /locus_tag="VFQ79_13315"
     CDS             complement(333..2096)
                     /locus_tag="VFQ79_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0140694.1"
                     /translation="MTNKNGFSGVLLAATLCCFSVLANGAVLQVGPGKPYSTPCAAIA
                     AAANGDTVEIEAAGEYLGDVCAWNKNDLTIRGVNGRPHIQANGRYVQSKGIWVAGGRN
                     LTIENIEMSGAELPPSIGTNGAAIRAEATNWTVRNCYFHDNQNSILESNVAGSNILIE
                     YTEFARNGHTSGQSHNLYIGHAASLIYRFNYSHDSVEGHLLKTRAAVNWILYNRLSGE
                     SGTGSYEIDVPNGGTTYVIGNLIQQGPDSHNSTLLSYGAENVHPQNPGHDLYVINNTF
                     VNQRPAGATFIRVDARGGVPALVSNNIFAGPGTLAAGLAPVLVANLQVSDPTQASFAD
                     YANFDYRLTAGSPAVNAGAEPGLAADGTPLAPVAQYVHPTAAEERITSEVIDIGAYEY
                     HDPTPPVITPVVQGPQGAGGWYTGGVTIGWSLSDPETGIASSTGCENTVLTQDTAGVT
                     LTCSAVNRAGLSSSASVTVKIDKTAPVIAGLPAPGCSLWPPNHKLVTVGTVTAYDALS
                     GLVPATFQVTGASNQPAEPASPDIVISPSGAGGFTVQLRAERLGNAGSRIYTLTATAA
                     DAAGNAATVVRTCTVPHDQGN"
     gene            complement(2134..2775)
                     /locus_tag="VFQ79_13320"
     CDS             complement(2134..2775)
                     /locus_tag="VFQ79_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0140695.1"
                     /translation="MLRKLLVCSVFLAGCAALQAQKYEFSVNGSYLRLNEKELGSIDI
                     EDPEDTDTTLKGEYGYGARITLNTSGYYGHELSYSQSRAKMRTVIRSVVSGNTVSEIR
                     EDRVMVQQLSYNFLIYFMPAGERWRPFVTGGLMGVQYGAPNFSEWPGGGARTYGGNYG
                     AGIKVRLFPHMLFRLDFRDNFAGRPYDLNFQDVTTAGGISRYMEGSAGFSITF"
     gene            complement(2807..4162)
                     /locus_tag="VFQ79_13325"
     CDS             complement(2807..4162)
                     /locus_tag="VFQ79_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0140696.1"
                     /translation="MKMQWEILVADDEEVMCESLAAWLREDGYLVETASSGREAVAKA
                     REKDYAIYFLDLKMPGGMDGIDAMMEIRRLHPDASIIIITAYATVDTAITAMKEGAQE
                     YIVKPCNPEEISLLVRRIIKVKNLQRENTILRKKLARQYHFQDIICKNPRMRAVVDMA
                     VQVANLKSTVLIRGESGTGKELVARAVHNSGDRAANKFVAVSCAALPEGLLESEMFGH
                     EKGSFTGAIAQKKGKFELADGGTIFLDEIGDISPKLQVDLLRVLQEKSFYRIGGSEEI
                     RTDARVIAATHVDLEQAVAEGKFREDLFYRLNVIEIRIPPLRERRDDIPLLADYFAER
                     ISREMGRDVIDISEGAYKLLMDHQWPGNVRELENAIERAIVTSKSKVLTEDDFAFLPR
                     SNGRRVWNVPADTTLQEMEKQAITATLERTQGNIKDAAAVLGIDRSTLYEKIKRYEIP
                     R"
     gene            complement(4159..4728)
                     /locus_tag="VFQ79_13330"
     CDS             complement(4159..4728)
                     /locus_tag="VFQ79_13330"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HEU0140697.1"
                     /translation="MHVIPVGRVEPRVIDTAQRCLADVLGCGTVLVPAMEDFEFAYDP
                     ERGQYSAPKLLQHLARHRLANSWRLLAITELDLFIPMLSYVYGQAQLGGALAVVSIAR
                     LRQEFHGFPADEGLLMARTAKEVLHETGHTLGLVHCRDETCVMSLSTNIRQVDVKRPA
                     FCADCRVRAVDAAAGGHRRQEILEAGVSE"
     gene            complement(4734..6374)
                     /locus_tag="VFQ79_13335"
     CDS             complement(4734..6374)
                     /locus_tag="VFQ79_13335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0140698.1"
                     /translation="MPRRLAQKLILSLTVIVLIIEAIFGFSNVKTQERELLDAMIVGA
                     DQLSKGITSATWHAMLLDNKESAYEVMQMIALKQGIDSIRMFNREGKVMFATRAEEQR
                     ESQAGKNSEACIGCHSTDQPKVNLDVSSRVRIVPAADGSRSLRMATPIYNEPACSQAA
                     CHAHPAGMKVLGVLDLALSLDPVDEQVANMKMRVLLVTGSQILLISVFIIFFTRYFLS
                     GPIGKLVEATKTVSAMQLDRPIEIGGRSEELDELARSFESMRVRLRTALAEINQFTHS
                     LETKVNERTEQLKVAHRKLLQTDRLASLGQLAASVAHEINNPISGVLNLSMLMQRILK
                     DDGIPPGRIADFRKYLSQVTGETSRVGRIVSDLLTFSRRSKPQRALADLNRIVRSTVS
                     LTSHKLKLAGVAVEMNLAEDLPQVPCDASQIQQVVLNLVLNAAEAMHARAEGLVRIGT
                     RVEEKASAVLLSVEDSGEGIRPEHLAKIFDPFFTTKPEGKGVGLGLAVSYGIVEAHGG
                     DIEVVSKVGEGTTFVVRLPLGEVPAPSEPVGEARAAGV"
     gene            complement(6439..7116)
                     /locus_tag="VFQ79_13340"
     CDS             complement(6439..7116)
                     /locus_tag="VFQ79_13340"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU0140699.1"
                     /translation="MRHATGACAAVAAGLLAAALVAQGQQRGRVEDLAAGQFLVASRK
                     LPDPNFAEAVVLLIQYDDQGSVGLIVNRPSKTPLSRLFQDIEAAKERTDPVYRGGPVA
                     PFGAMALLRTRSRPEGAQHIFADVYRSADKDVLEKAITDGTGPDAFRVYAGYAGWARH
                     QLEREVRMGAWHIFPARVDTVFDPKPEEIWPRLIRRTELQIAGSAPGPIPLADARGSV
                     PSERPSR"
     gene            complement(7116..7880)
                     /locus_tag="VFQ79_13345"
     CDS             complement(7116..7880)
                     /locus_tag="VFQ79_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0140700.1"
                     /translation="MSYKLAMGQILVEGGCPEANLERAAAMIEAAGRAGCKIVVLPEC
                     LDLGWTDPSAREIAEPIPGRYSDVLSREASDSALYVAAGLSERAGSRVYNAAVLIAPN
                     GEIVLKHRKINELTIAQDLYSMGDRLSVVETPYGTAGITICADNFPDSLVLAQALARM
                     GCQILLSPCAWAVEANHDNSLEPYGALWRGAYETLTGLYDLTVAGVSNVGWLRGGPWM
                     GRKCIGCSLAVGPGGRVLAQGPYGESAESLIVVDVP"
     gene            complement(8020..8370)
                     /locus_tag="VFQ79_13350"
     CDS             complement(8020..8370)
                     /locus_tag="VFQ79_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU0140701.1"
                     /translation="MDNARVFRMPFASVYPHYITKAEKKGRTKEEVHAIIHWLTGYDE
                     QTLQQQIDKKVDFETFFARAPWINPNVSKITGVICGYRVEEIEDKLMQQIRYLDKLVD
                     ELARGKAMDKILRK"
     gene            complement(8498..8938)
                     /locus_tag="VFQ79_13355"
     CDS             complement(8498..8938)
                     /locus_tag="VFQ79_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0140702.1"
                     /translation="MRPQPLIAVSDVEASSRWYQRLFGCRSNHGGPVYEQLVLNGRLM
                     LQLHSFEVEHHHVPIGNRDDKPYGNGVLLWFEVDDFDSVLQRTTEMGVEIVMPRHRNP
                     PDEEGGPNHWECWMRDPDGYIVVAASPYGSADGTWHPGPHPFGI"
     gene            complement(8986..9888)
                     /locus_tag="VFQ79_13360"
     CDS             complement(8986..9888)
                     /locus_tag="VFQ79_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0140703.1"
                     /translation="MIHSFIARYLPCFFFLAASGILVASSSSAPMGKFYIVGMGTAPD
                     LITVRGQNVIARADILMAEEGAFQTMWAGLARGKGVWEWSHNLRRFYGADPKTLKDPN
                     QRTLAENMEKARRQLIGKIVSAVAAGKVVACLQGGDPMMYGMTLFLEMLPPNIPSEVV
                     PGVGAFQAASAALKMSPPYGYDTNAVILTMADWPGRVDVNEKLMATGSTMVFYTMNLN
                     YPSLFAQLKRYYPADTPVAVVSNAGDPNHQKVTRSTVSRFLEEVDPRSLPANSHMLFV
                     GKFLSVGQARKDFTPRITTRAGGK"
     gene            10080..10334
                     /locus_tag="VFQ79_13365"
     CDS             10080..10334
                     /locus_tag="VFQ79_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140704.1"
                     /translation="MRARGVLAVSIDSDSALVRRFIEENGYTMPVIVDRTLADKVDFT
                     TGPPQNYLIDREGRKLVEPVKGSGEEWLKLVRELMDRLAR"
     gene            complement(10389..13880)
                     /locus_tag="VFQ79_13370"
     CDS             complement(10389..13880)
                     /locus_tag="VFQ79_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140705.1"
                     /translation="MRIQWLVVLLGLASCQALPAQETRGSIQGRVTDTSGAVIPGTVV
                     HATNKATNVTVSTRSNAEGNYGIPYLVSGIYVLKAEVGGFKTFIRDNIEIRIDDRLNI
                     PIQLQIGELAEVLTVTAETPLLDSTNASMGQVIDQRRIAELPIAHGNPYLLMQLSAGV
                     VYTQNAGLDRPFEPTHIVGYSMDGVKANKSEITMDGVQNSTVKRGTTDTIAGYTPPAD
                     IVQEFKIQTMPFDASVGHTQGGVTSITLKSGGNQLHGTAYWGVLDPSLNANLFFANKA
                     GQPRGEFDYNRWGGSLTGPVYIPKVYNGKDRTFFTYGYEGIHESRPMGSAYGTGTLTV
                     PTAAQREGDFSALLKLGSQYQVYDPATRKQAPNGRTVVDPLPGNIVPSSRISPIAKSI
                     LNYWSLPNAPGTADGLNNLIRVNDPEAITYYNHISRVDHAFNDKHRMFARVNTYRRFS
                     YSSDWFRSAATGTDSEWKQHAASLDHVYQINPTTLLNLRAGYFRLQIQQTPLNEKGFD
                     LASLGFPPIYTDAIDRSYSAFPYITITGYTATPNTWFRDPMANASLEAHLTAVRGNHT
                     LRFGGDGRQYRTNHYVWNRASTGRFNFDNTWTRGPFDNSPVAPIGQAAAALMLGLPSG
                     GYVERNASFAEQSTVWSLYMQDDWRVTPSLTINAGLRYEIEGPLTERWDRSIRGYDFD
                     TPNPLNDVVRANYARNPIAELPPEQFRLMGGVTFAGVNGQPRTLFTRDKNNIMPRIGF
                     AYQIGPRTVLRGGYGVYFGQLGLQRGDVKQEGFSYATNLVPSLDSGLTFVATLANPYP
                     TGIEDPRGAADGLMTFTGRAITFFDENPEASRQQRWQLSIQRELPQRVLLELGYVGNH
                     ASSIQTTRDLRGLPLQYLSTSPYRDTAHINWLSQQVPNPFAGLLPGTGLNGANTSRSY
                     LLSSGDYPHFTDISTTQYDGYSWYHALQVRTERRFASGWTVTANYLWSKNMEALSRLN
                     GPYSPLEYVVSDQDRPHRVVVSAIWELPFGRGKRYLSSMPGVVDKFFGGWQVQGIYTA
                     QSGQAIGFGNALFIGDIHDITLPKSERKPERWFNTDAGFERASNMQLGNNYRLMPSRF
                     NDVRTDGINSFDLSALKNTRIGERFNVQFRGEFLNAFNHTLFAEPNTTPTSSAFGQVT
                     AQRGYPRRIQLGLKFLF"
     gene            14024..14233
                     /locus_tag="VFQ79_13375"
     CDS             14024..14233
                     /locus_tag="VFQ79_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140706.1"
                     /translation="MSEADIQIVPDEAGQPTAVIVPIALWKEIVSERETAYLLKSETM
                     RQRLLAALERGNGVPLDDAVTKLGL"
     gene            14315..15256
                     /locus_tag="VFQ79_13380"
     CDS             14315..15256
                     /locus_tag="VFQ79_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEU0140707.1"
                     /translation="MRMAAITLLRVLCQYEMDMLDAASKFRVGLIQMSCSAKPEENLE
                     KAQWKIREAAGKGAQIICLPELFRSQYFCREEDAALFALAETIPGPSTDVLTRLAAEL
                     KVVIVASLFEKRAQGLYHNTAAIIGAHGNIQGIYRKMHIPDDPLYFEKFYFTPGDLGF
                     PNFDTAFGKIGVLVCWDQWYPEGARLSSLAGANILFYPTAIAWHPSEKEQYGAAQLDA
                     WTTIQRAHAIANGIYVAAVNRIGFEGPSDRGLEFWGSSFVADPFGQVIAQAPVDREET
                     LVAECDPRKIDEVRRNWPFLRDRRIDAYGPLVYRWLG"
     gene            15244..16323
                     /locus_tag="VFQ79_13385"
     CDS             15244..16323
                     /locus_tag="VFQ79_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEU0140708.1"
                     /translation="MARLNQRTPAALGYRMPAEWEPHEATWIAWPHERSDWPGKFAPV
                     PWIYCDIVRHLARLEKVRILIASEEAERQASRMLEKSGADLDAVEFLRFETDRSWCRD
                     YCPIFVKNRAGDVAITDWHFNGWAKYENWTRDDKAPAAIAKKLGMPLFRPVSNGRRVV
                     LEGGSIDVNGAGLLLTTEECLLSEVQSRNPGTGRAGMERILADHLGIHKVLWLKNGIA
                     GDDTHGHVDDIARFVNPTTVVAASEDNREDTNYANLRENLELLRGMKDEEGCPLKIVK
                     LPMPAPVWFDGQRLPASYANFYIANGIVLVPVFNDPNDRIALNTLARLFPDREVTGIY
                     SRDLVLGLGTLHCLTQQQPAAGALS"
     gene            16405..16965
                     /locus_tag="VFQ79_13390"
     CDS             16405..16965
                     /locus_tag="VFQ79_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEU0140709.1"
                     /translation="MKYWAQLTGTGLFALTMACGPSTPPATETKEEAPAAMLTAKAEL
                     RSSKGEDVGQATFTQTNGSVRLSVEVKNMPPGTHAIHVHGVGQCDAPDFKTAGGHFNP
                     GAKQHGFENPQGHHVGDMPNMTVGQDGTGTFEYTIEGASLAATGENSLFHPGGTALVV
                     HADPDDMKTDPAGNAGGRIACGVVTR"
     gene            17003..17923
                     /locus_tag="VFQ79_13395"
     CDS             17003..17923
                     /locus_tag="VFQ79_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0140710.1"
                     /translation="MHWIWNLGGLTWRELARRVYGQILKDDVLGRAAQLSYYFLLALF
                     PLMVFLMVVLGYLADTGTWMRERMLEYLAHVVPPSAFTLILDTLDAISNGGGSGKLSL
                     GLLGAIWAASAGMNAVIRGLNIAYGVKEARVWWRARLVAITLTLALSISSLFTLTLFL
                     WGDRIGSFVAEWLGHTALFQTIWSWAQPVLVVAFLMATFSAIYRFAPNLEEQKWVATL
                     PGAVLGVTIWLLASYGLRLYLVYFTNYNKTYGSVGAVIILMLWFYVTSLALLIGGEIN
                     SEIENAAAERREPGAKFFGERMPHEHAWRG"
     gene            complement(17925..19610)
                     /gene="ggt"
                     /locus_tag="VFQ79_13400"
     CDS             complement(17925..19610)
                     /gene="ggt"
                     /locus_tag="VFQ79_13400"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0140711.1"
                     /translation="MSKYLLMCLVLVSSVGAQERSTARSMVVTTQGIVATAQTLASQA
                     GAQVLARGGSAVDAAIAANAALSVVEPMMNGMGGDLFVIYREAKTGKLYGLNASGWAP
                     KALTVEYLKSKGIAKMPELGMNSVTVPGCVDGWEKLHKRFGRLAWSELFRPAIYYAEN
                     GFPVTEIIQGHWQDGMQKLSSDENARRLFLKDGKPPAVGEIFRNVQLAKALKLVSDSG
                     ADAFYRGPIAKAILQTSDRLGGVMNAADLADFQSEWVEPVMTDYRGWKVYELPPNGQG
                     LAVLEMLNIFERFPLAKMDAADALHVKIEAQKLAYMDLVKYVADPRTACIPVGGLISK
                     PYAEQRAALIRMEKASCEVKPGNAPSTNGDTTYLSVVDREGNIVSLIQSIYLAFGSGV
                     MVEGMGFHLHNRGGLFVLEDGHPNVLAGHKRPFHTIIPGYMEKGNTHIGFGIMGGYNQ
                     AQAHAQFVSNVVDAGMNIQEALEAPRFTKLTFGGCDVMVENRVPQEVRDELSKRGHQF
                     EVRAAYSGRVGGGQAVIYDSGTQVKFGASSPRKDGAAIPQQDPYFPSPPQFVR"
     gene            complement(19912..21363)
                     /locus_tag="VFQ79_13405"
     CDS             complement(19912..21363)
                     /locus_tag="VFQ79_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0140712.1"
                     /translation="MSEHTALDVLSPPKHKGRILVVDDEADIRESLETLLSLENYQVD
                     LAVNGTDGLRKMENTAYDLVLLDLMMPDRSGMEVLKEVRERDTGTPIFMITAYGSIEV
                     AVSAIKSGANDYFPKPWDNEKLLIEIEGMIARGRLERENTQLKRVLKQRYSFPNIVGK
                     SDRMLRVLDLVTQVAPSRTTVLITGETGTGKELIAKAIHANSPRADNMFFAVNSGGLP
                     PDLLESTLFGHVKGAFTSAVTSQKGYFEIANRGTIFFDEIGTISLETQTKLLRVIQER
                     EFRPLGSTEIVRVDVRIIAATNADLKRLVEEGRFREDLYYRLNVINVPLPPLRDRKED
                     IPLLIRHFFDRYCRENERFLDSVGHSVLEFEPDAMQILMDYNWPGNVRELENAVERAV
                     VLASGPSVTVDVLPDQLLQAGGIRIRRDDKGYLPPDASLFEIVADFERRKIIEALEGV
                     NWSQTEAAEQLHIPLSTLNQKIKRLNIEIRKKA"
     gene            complement(21356..24301)
                     /locus_tag="VFQ79_13410"
     CDS             complement(21356..24301)
                     /locus_tag="VFQ79_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0140713.1"
                     /translation="MRELKDQLVNALLVVLTAAAVISAALNFQQQKKVKLPDDGAIWV
                     DRNGRVEALHIVPGSQADNRGLRAGDIIVAINGAPVRNALDVTRILVGIGGWRSADYL
                     IHRQGVEVKVPIILGEREPQGATYYQYLVALAYLSIGLFVYFRRGGAPKARHFYILCL
                     ASFVLFAFHYTGKLNNFDKVIYWGNVAAGVLAPTIFLHFCLTFPERGKWFRSRWRIAL
                     LYVPGLLLVAAYVGFSSGTIRIAVSLVEVRWMLDRLWLLLMAGLYLAGALVLTFQYHS
                     HREDPIVRQQLKWLRNGALFGVVPFTLFYVLPYVLGAVPGRVMDAMVFSLLLVPLTWA
                     YAILRYRLMDVDVIFQQGYVYTAATLSVIAIFYALILSVWGRMEELNSTALVSLLLIA
                     TFIFQPIRKWLQEQFDRYIFYKDRYDYRRTLIEFARELGSETDLDATLNSVADRLRRT
                     LSIQYIAFFLLGEEDGRFYLKMNTGRLPRNGQLSTGDLDLSFLSAESGKPYLFFERTR
                     HALDVVSTHWPASVRDTIANLDLTYYLPCQVRGRTIAYIGASRTEEDDFLPSDDIELL
                     QTISGYVGIAVENARLYRTLQRKVDEYERLKEFSENIVESINVGILAADLADRVESWN
                     TQMERLTGIPREYAVGRTLTELIPRDLCDRFDSVRGETGIHHIYKFALRAHSLPAQVA
                     ELAAGAYAGGGTNGNGSGTHEVAPDGAAEAAGGAILNIAVAPLVSRDLEQIGRLIIFD
                     DITDRTELEQKLVQADKLSSIGLLAAGVAHEVNTPLAVISTYAQMLAKQIPGDDQKSK
                     LLDKIAKQTFRASEIVNSLLNFSRTSTTSYDDVDLNKVLRETVALLEHQLQKAGVSVS
                     LHLEDGMPAIRGNTGKLQQVFLNLVLNARDAMNSGGVLYIRSCSDRGLARVDVSDTGQ
                     GIASENLARIYDPFFTTKASRKGTGLGLSVTYGIVKEHGGSIEVQSEVGIGTRFHLEF
                     PLSRKPVNV"
     gene            24528..25169
                     /locus_tag="VFQ79_13415"
     CDS             24528..25169
                     /locus_tag="VFQ79_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0140714.1"
                     /translation="MHRLFMLLLLSFPAFGQFVFGVKGGVPLSDAFDIARAPSPAREF
                     SSATRRYTIGPTAELRLPFGLGIEFDALYKRLGYSSSGSTANLTFHSETTANSWEFPL
                     LVKYRAPGALARPFVLGGVSFHRLSDVKQFTVETFQTVTGDRTSTTSEPEELRDKSDQ
                     GFVLGAGLELRAPVIRISPEIRYTRWGSENFLDALNILRSNKNQWEFLIGLTF"
     gene            25201..25782
                     /locus_tag="VFQ79_13420"
     CDS             25201..25782
                     /locus_tag="VFQ79_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0140715.1"
                     /translation="MSSPQQDVTRLLVNWSKGDQQALEDLTPLVYGELRRLAGRYLRR
                     ERSDHTLQSTALVHEAYLRLVDQRNVKWQNRAHFFGVAAQLIRRILVDYARSHQTLKR
                     GAGACKLSLDEAIGAPEKRDLNVVALDDALDDLAKIDPQQSRIVELRFFAGLSIEETA
                     EVLKISPATVKRDWVVAKAWLYRNISGRESGDT"
     gene            25772..>26240
                     /locus_tag="VFQ79_13425"
     CDS             25772..>26240
                     /locus_tag="VFQ79_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0140716.1"
                     /translation="MTPERWRQVKEILATSLELEPETRNTYLDTVCGADPGMRAEVES
                     LIAAYEEAGDLMESPAGGKKFSLSSSSIETDPLRRIGPYFIVERIAQGGMGAVYRCYR
                     EDDPDRKTVAVKVLKPGADSSFVLRRFSHEREIVANLHHPNIARFLDGGSTDDG"
BASE COUNT         5175 a         7919 c         7837 g         5309 t
ORIGIN      
        1 ttggaaacgg gcggtccggc cgccggctgg tatctgaaaa tcaaaccgga ttcctccgcc
       61 ggtgatgccc acctgctgcg cggcgcggaa gtgcagccgt gggtgatgag ggagctcgag
      121 cggctgcaac tcgcattcgc acccaccgcg agcctggccg atggcggtgt gttgagcgag
      181 gatctgtcga agaacgtccc tcgcgacgag tgggaactcg cctgcggaga gatgttcctg
      241 aatccgtaga gtccgcttgc tcgcgcgcgc ggctcagaaa ggtgatcgca tttttgcgag
      301 cgtttccgag ccgcgaccgc aaggaagcgg tgttagttgc cctggtcgtg gggcacggtg
      361 cacgttctta ccacggtggc ggcgttaccc gctgcgtccg ccgctgtcgc ggtgagagta
      421 taaatccggc tgccggcatt ccccaaacgc tctgcccgga gttgcaccgt gaacccgccg
      481 gcgcccgacg gggatatgac gatgtcggga ctcgcgggct cggcaggctg attgctcgcg
      541 ccggttacct ggaaggtggc cgggaccagt cccgatagcg cgtcgtacgc cgtcaccgtg
      601 cccacagtga caagcttgtg attgggaggc cacagcgagc agcccggcgc cggcaggccc
      661 gcgatgaccg gcgcggtctt gtcgattttg accgttaccg atgccgaact cgacagaccc
      721 gcgcggttca ccgccgagca ggtaagggta acgccggccg tgtcctgggt gagaaccgtg
      781 ttctcgcagc cggtagacga agcgatcccc gtctcggggt ccgataatga ccagccgatc
      841 gtgacgccgc cggtgtacca gccaccggcg ccttgggggc cctgcacgac cggcgtgatc
      901 accggcggcg tgggatcgtg atattcgtaa gcgccgatgt cgatgacttc cgacgtaatg
      961 cgctcctccg cggccgttgg atggacgtac tgcgcaaccg gcgccagcgg agtgccatcg
     1021 gcggccagcc ccggctccgc cccggcattg acggcgggcg agccggccgt caggcggtag
     1081 tcgaaattgg catagtctgc gaagctggct tgcgtggggt cgctcacctg gagattcgcc
     1141 acaagcacgg gggcgagccc ggcggccaga gtgcccggcc cggcgaaaat attgttgctg
     1201 accagcgccg gtacgccgcc ccgcgcgtcc acacggatga acgtggcgcc ggccgggcgc
     1261 tggtttacga aagtgttgtt gatcacatag agatcgtggc ccggattctg ggggtgaacg
     1321 ttctccgcgc cgtaagacag aagcgtggag ttgtgcgagt cggggccttg ttgaatgagg
     1381 ttcccgatca cataagtcgt gccgccgttg ggtacgtcaa tctcgtagct tcccgtgccg
     1441 gactctccgc tgaggcggtt gtagaggatc cagttgacgg cggcccgcgt cttcagcaag
     1501 tgaccctcca cggaatcgtg cgagtagttg aagcggtaga tcagggaggc ggcgtgcccg
     1561 atgtacaggt tatgggactg gccgctggta tggccgttgc gggcgaattc cgtgtattcg
     1621 atgaggatat tgctgccggc gacgttactt tccaggatgg aattctggtt atcgtggaag
     1681 tagcagttgc gaaccgtcca gttggtggcc tcagcgcgaa tggcggcgcc gttggtgccg
     1741 atggaaggag gcaattcggc gcccgacatc tcgatgttct cgatcgtcag gtttctgccg
     1801 cccgccaccc agatgccctt actctgaacg tatctgccat tcgcctggat gtgcgggcgc
     1861 ccgttgacgc ctcggatggt gagatcgttt ttgttccagg cgcagacgtc gcccaggtat
     1921 tcgcccgcgg cttcgatttc gacggtgtcg ccgtttgccg ccgcggcgat tgcggcacag
     1981 ggggtgctgt aaggcttgcc cgggcctacc tggagaacag cgccgtttgc caggacgctg
     2041 aagcagcaca acgtggctgc caggagtaca cccgaaaacc cgttcttatt ggtcatcgct
     2101 atttctccgc agttggagcc ggaatcgaac tactcagaag gtgatcgaaa acccggcgga
     2161 accttccatg tagcgcgaga ttccgccggc cgtggtgaca tcctgaaaat tcaagtcgta
     2221 gggacggccg gcgaaattgt cgcggaagtc gagccggaac agcatgtgcg ggaataatcg
     2281 aaccttgatt cccgctccat aattgcctcc gtaagtgcga gcgccgcctc caggccattc
     2341 cgaaaaattc ggagcgccat actgaacgcc cattagtcct ccggtgacga aaggccgcca
     2401 tcgttcgccc gcgggcatga agtagatcag gaaattgtac gaaagctgct gaaccatgac
     2461 ccggtcttcg cggatctcac tgaccgtgtt gccggaaaca acactgcgaa tcaccgtcct
     2521 catcttcgcg cggctctggc tgtaggaaag ctcgtgacca tagtagccgg acgtattcag
     2581 agtaatcctg gccccatagc cgtactcccc tttgagggtg gtgtccgtgt cctcgggatc
     2641 ttcgatgtca atggacccga gttctttctc gtttagacgc aggtaagagc cattaacgga
     2701 aaactcgtac ttctgggcct gcaatgccgc acaaccggca agaaaaacag aacagacgag
     2761 cagttttcgt agcattctct ctaactaatt tacactgggg ccgaattcag cgagggatct
     2821 cgtaccgttt gatcttctcg tacagcgtgg aacggtcgat gccgaggacg gccgccgcgt
     2881 ctttgatgtt cccctgggta cgctccagcg tcgctgtgat ggcctgcttt tccatttcct
     2941 gaagcgtcgt gtccgccggc acgttccaaa cgcgccttcc gttggatcgc ggcaggaaag
     3001 cgaaatcgtc ttccgtcaac accttgcttt tggaggttac aatagcgcgc tcgattgcgt
     3061 tttccagctc acgtacgttt cccggccact gatggtccat cagaagcttg taggcgcctt
     3121 cggagatgtc gatcacatcg cggcccatct cccgcgagat gcgttcggcg aagtaatcgg
     3181 ccagcagcgg gatgtcgtcg cgccgctccc tcaacggtgg aatacggatc tcgatgacat
     3241 tcagccggta aaaaaggtct tcgcggaact tgccttcggc gaccgcctgc tccaggtcca
     3301 cgtgcgtggc ggcgataacc cgcgcgtccg tgcgaatctc ctcggacccc ccgatgcgat
     3361 aaaagctttt ttcctgtaag acccgcagca ggtccacttg aagcttcggt gagatgtcgc
     3421 cgatctcgtc caaaaaaatc gtgccgccgt cggccagctc gaacttcccc tttttctggg
     3481 cgatggcgcc ggtgaacgag cccttctcgt ggccgaacat ctccgattcc aacaaccctt
     3541 cgggcagggc cgcgcaactg acggccacaa atttgttcgc cgccctgtcg ccggagttat
     3601 ggacggcgcg agcgaccagt tccttcccgg tgccgctttc cccgcggatg agcaccgtgc
     3661 tcttgagatt cgccacctgc accgccatgt cgacgacggc gcgcatgcgc gggttcttac
     3721 agataatgtc ctggaagtgg tactgccgcg cgagtttctt ccgcaggatc gtattttcgc
     3781 gctgaaggtt tttgactttg atgatgcggc gcaccagcag cgagatctct tcgggattgc
     3841 acggcttcac aatgtattcc tgcgcgcctt ccttcatggc cgtgatcgcc gtatcgacgg
     3901 ttgcataggc ggtaatgatg atgatggagg cgtccgggtg caggcggcgt atctccatca
     3961 tcgcgtcgat gccgtccatt ccgcccggca tcttgagatc gaggaaatag atcgcgtaat
     4021 ccttttcgcg cgctttcgcc accgcctccc tgccggacga ggcggtctcg accaggtagc
     4081 cgtcctcccg gagccacgcg gccaacgatt cgcacatcac ctcttcgtcg tcggcgacga
     4141 gaatttccca ttgcatcttc attctgaaac tcccgcttct aaaatctcct gtctccgatg
     4201 cccgcccgcg gcggcgtcca ccgcccgaac gcggcagtcc gcacagaaag ccggccgctt
     4261 cacatccacc tggcgaatgt tcgtagagag cgacatgacg caggtctcgt cgcggcagtg
     4321 caccagtccc agggtatgcc ctgtctcgtg caacacctct tttgccgtgc gcgccatcag
     4381 caacccttca tcggcgggaa agccatggaa ttcctgccgc agtctggcga tcgataccac
     4441 cgccagtgcg cctcccagtt gggcctgccc gtaaacgtag ctgagcatcg gtatgaacaa
     4501 atccagttcc gtaatcgcca gcaaccgcca ggaattcgcc aggcggtgcc gcgcgaggtg
     4561 ctgcaacagt ttcggcgcgc tgtactgtcc gcgctccggg tcgtaggcga actcgaagtc
     4621 ctccatggcc ggcaccagaa ccgtgccgca acccagtaca tccgccaggc acctctgagc
     4681 cgtatcgatg acgcgcggct cgactctgcc aaccggaatg acatgcatgc atctcacacg
     4741 ccggcggccc tggcttcccc aaccggctcc gaaggcgcag gcacctcgcc cagcggcagc
     4801 cggaccacaa atgttgtgcc ctccccgacc ttgctcacca cctcgatgtc gccgccgtgc
     4861 gcttcgacaa taccgtagga gacggcgagg cctaagccca cgcctttgcc ttccggtttc
     4921 gtcgtgaaga acgggtcgaa gatcttggcc agatgctccg ggcggatccc ttcacccgaa
     4981 tcttcgaccg aaagaagaac cgcggatgcc ttttcctcca cccgcgtgcc gatgcggacc
     5041 aggccctcgg cacgggcatg catcgcctcg gcggcattca acaccaggtt cagaacaacc
     5101 tgttggatct gtgaggcgtc gcagggaacc tgtggcaggt cttccgccag attcatctct
     5161 accgccactc ccgccagctt cagcttgtgc gaagtcagag atacagtgga acgaacaatc
     5221 cgattgaggt cggcgagcgc gcgttgaggc ttggatcggc gtgaaaacgt gagcagatcc
     5281 gacacgatcc gtcccacgcg cgaggtctcc ccggtaacct gcgaaaggta cttgcggaag
     5341 tcggcgatgc ggccgggcgg aatgccatcg tccttcagaa tgcgctgcat caacatcgaa
     5401 aggttgagaa caccggatat ggggttgttg atctcatgcg ccacgctggc cgcaagctgt
     5461 cccagcgacg ccagccggtc ggtctgaagc agtttccggt gcgccacctt caactgttcg
     5521 gtacgttcat tcaccttcgt ttccaggcta tgcgtgaatt gattgatttc ggccagcgcc
     5581 gtacgaagcc tcacccgcat cgactcgaac gaccgcgcca gctcgtccaa ctcctcgctg
     5641 cgcccgccaa tttcaatggg ccggtcgagt tgcatcgcgc taaccgtctt ggtggcctcc
     5701 accagcttac cgatgggacc actgaggaag tagcgcgtaa aaaagatgat gaatacgctg
     5761 atcaggagaa tctggcttcc ggtcaccagc agcacgcgca tcttcatgtt ggccacctgc
     5821 tcatccaccg ggtcgaggct cagggccagg tcgagtaccc ccaatacctt cataccggcg
     5881 gggtgtgcgt ggcatgccgc ctggctgcaa gcgggctcgt tatagatcgg agtagccatt
     5941 cgcaggcttc ggctgccgtc agccgccggc acaatccgga cccgcgagga gacgtccagg
     6001 ttcaccttag gctgatcggt ggagtgacac ccgatgcacg cctccgagtt cttgccggcc
     6061 tgcgactcgc gttgctcctc ggcgcgcgtc gcaaacatca cctttccctc gcggttgaac
     6121 atgcgtatgc tgtcgatccc ctgtttgagg gcgatcatct gcatcacttc gtacgcggac
     6181 tccttgttgt ccagcagcat ggcgtgccac gtggcgctgg tgatgccctt tgaaagctgg
     6241 tccgcgccga caatcatcgc gtccagcagt tcgcgctcct gcgtcttgac attgctgaat
     6301 ccgaagatcg cctcgatgat caggacgatc accgtaaggg acagaatgag tttctgcgcc
     6361 agccgtctgg gcacgcaccg tcctccataa aaggagttcc atacctatta tgcctgaatc
     6421 ttgccggagt ggtacatatc agcgggaggg ccgctcgctc ggaacggagc cgcgcgcgtc
     6481 agcaagcggt atcgggcccg gcgccgaccc ggcgatctgc aattcggtcc ggcgaatgag
     6541 ccggggccag atctcctccg gcttcggatc gaaaaccgta tccactctcg ctgggaaaat
     6601 atgccaggcg cccatccgga cttcgcgttc gagctgatgg cgggcccagc ctgcatatcc
     6661 tgcgtacaca cgaaaagcgt cggggccggt cccgtctgtg atcgctttct cgagaacgtc
     6721 tttatccgcg cttcggtaga catccgcgaa tatgtgttga gcgccttcgg gcctggagcg
     6781 cgttcgcaga agtgccatcg cgccaaacgg cgcgacggga ccgccccggt aaacgggatc
     6841 ggttcgctct ttcgcagcct cgatatcctg gaacaaacgc gagagcggag tcttgctcgg
     6901 ccggttgacg atgagcccga cgctcccctg atcgtcgtac tgaatcaaaa ggaccacagc
     6961 ctcggcgaaa ttcgggtccg gcagtttacg gctcgccacg agaaactgcc cggcggcaag
     7021 atcctccact cgcccgcgct gctgtccctg cgctacaagc gccgctgcca gaagaccggc
     7081 agctaccgcc gcgcaagctc ctgtggcatg gcgcatcacg gcacgtccac cacaatcagg
     7141 ctttcggcgg attcgccata gggcccttgc gccagcacgc ggccgccggg accgacggca
     7201 agagagcacc cgatacactt gcggcccatc caaggcccgc cgcgcagcca gcctacgttg
     7261 ctgacgccag ctacggtgag atcgtaaagc ccggtgagag tctcataggc tccacgccac
     7321 aacgcgccgt aaggctcgag ggagttgtcg tggttggctt caacggccca ggcgcaggga
     7381 gagagtaaaa tctggcagcc cattcgcgcc agtgcctgcg ccagcaccag cgaatccggg
     7441 aaattatccg cgcagatcgt tataccggcg gttccatacg gcgtctcgac taccgaaagc
     7501 cggtcgccca tcgagtacaa gtcctgagca atcgtaagct cgttgatctt acggtgcttg
     7561 agtacgatct cgccattagg cgcgatgaga acggccgcgt tatacacacg tgagccggcg
     7621 cgttcgctca gtccggcggc gacatagagc gcggaatcgg acgcttcgcg gctaagcacg
     7681 tcgctgtagc ggcccggaat cggctcggca atctcgcgcg ctgaagggtc ggtccaaccc
     7741 agatcaaggc attcgggcag caccacgatt ttgcagccgg cccgtccagc ggcctcgatc
     7801 atcgcggccg cccgctccag attcgcctcc ggacagccgc cctccaccag tatctggccc
     7861 atcgccaact tatagctcac gctacatgcc agtatgaaat agatgtagcg ttgggtgctg
     7921 agaacaaggt ttccgccgca aagctactca ggagcggggc cgcgattacc aacatcggga
     7981 acgcctcgga aacgacgatg accacaccaa cgcccttgct cacttcctca ggatcttgtc
     8041 catggccttt cccctggcca gttcatccac cagcttgtcc aggtaacgga tctgttgcat
     8101 gagcttatcc tcgatctcct ccacgcgata gccgcagatc acgccagtga tcttggacac
     8161 gttcgggttg atccatggcg cccgggcgaa gaaagtctcg aagtcgacct tcttgtcgat
     8221 ttgctgttgc agcgtctgtt catcgtagcc cgtgagccag tggatgatgg catgcacctc
     8281 ctccttcgta cgacccttct tttccgcctt ggtgatgtaa tgcgggtaga cgctcgcgaa
     8341 cggcattctg aatactctcg cgttgtccat agactcgttg ctcctccatc ccacttttga
     8401 agtcgcgttc ccggaatatc ggcaaggacc cggcacgacg agggtaaact cacgatccgc
     8461 acataagtgc gagttggtgc cggcgacctc cccggattca tataccgaaa gggtgagggc
     8521 ctggatgcca ggtcccatcg gcggatccgt aaggactggc agctaccacg atgtagccgt
     8581 ccggatcacg catccagcac tcccagtgat tcgggccccc ttcttcgtcg ggaggatttc
     8641 gatgcctagg cattacgatc tctacgccca tttccgtcgt gcgttgcagg acagagtcga
     8701 aatcgtcaac ttcaaaccac aacaagaccc cgtttccata gggcttgtca tcccgattgc
     8761 cgattggaac gtgatgatgc tctacttcga aactgtgcaa ttgaagcata agccggccat
     8821 tcaagaccaa ctgttcataa accggaccac catggttgct tcggcagccg aacagacgct
     8881 gataccaccg gctgcttgct tccacatcac tcaccgcgat cagaggttga ggacgcatgc
     8941 cagccagagt aacaagttgc ggcgatgcgg ttcaccccgc ccacatcact ttccgcccgc
     9001 ccgagtcgtg atgcgcggtg tgaagtcctt ccgcgcctgt cccacactca ggaacttgcc
     9061 cacgaacaac atgtggctgt tcgcaggtaa gctccgggga tccacctctt cgagaaaacg
     9121 ggagacggtg ctgcgcgtca ccttctggtg gttcggatcc cccgcgttgg acaccacggc
     9181 caccggtgta tcggcggggt aatagcgctt caactgcgcg aacagtgacg gatagttcag
     9241 gttcatggtg taaaagacca tggtgctgcc ggtggccatc aatttctcgt tgacgtccac
     9301 gcggcccggc cagtcggcca ttgtcaggat gacggcattg gtgtcgtaac cataaggcgg
     9361 actcattttc agagcggcgc tggcagcctg aaaggcgccg acgccgggca ctacttcact
     9421 gggaatattg ggtggcagca tttccagaaa gagcgtcatc ccgtacatca tcgggtcgcc
     9481 accctggagg caggcaacta cttttccggc cgctacagcg gagacaattt tcccgatcaa
     9541 ctgacgacgg gctttctcca tattctccgc gagtgttcgc tggttgggat ctttcaacgt
     9601 tttcggatcg gcgccgtaaa atcttctgag gttatgggac cattcccaaa cccctttacc
     9661 ccgggctaac ccggcccaca tcgtctggaa ggcgccctct tccgccatca ggatgtccgc
     9721 ccgggcaatc acgttctgcc cccggacagt gatcagatcc ggagcggtgc ccattcccac
     9781 gatgtagaac ttgcccattg gcgcggacga agaggaagca acgaggattc cgctcgccgc
     9841 gaggaaaaag aaacacggaa gatatcttgc aataaacgaa tggatcatct atgccagtat
     9901 gagacaggta gggcgttgga tgttgcatcg aagagagggt cctacgagcg gaagatccgg
     9961 ttggaggacg tgccgcgtct cgtggaggat gctttgcggg agtccgagga agcggcgcag
    10021 tacgagcttc tggcgacgag cgaaagacca ggactcagat caatctggcg caaacccgga
    10081 tgcgggcacg cggcgtgctg gcggtttcga tagacagcga ttcggcgctg gtgcggcgat
    10141 ttatcgagga gaacggctac acaatgccgg ttatcgtcga ccggactctg gctgacaagg
    10201 tcgactttac gacgggcccc cctcagaact acctgatcga ccgcgaggga cgcaagctgg
    10261 tcgaaccggt gaagggatcg ggcgaagagt ggcttaagct ggtccgtgag ttgatggatc
    10321 ggctggcgcg ctgagggcgc ccgcctgctg ccgggcgccc cattttgtat tgccgtcttc
    10381 agcccgtatt agaacaggaa cttcaagccg agctgaatcc ggcgcggata cccgcgctgt
    10441 gcggtaacct ggccgaaagc cgagctggtc ggcgtggtgt tgggttcggc gaacagggtg
    10501 tggttgaacg cgttgagaaa ctctccgcgg aactgcacgt tgaacctctc cccaatgcgc
    10561 gtgttcttca gcgccgaaag atcgaaactg ttgatcccgt cggtgcggac atcgttgaac
    10621 cgcgacggca tcagccggta attgttcccc aactgcatat tggacgcacg ttcgaagccg
    10681 gcatcggtgt taaaccaccg ctcgggtttc cgctcgctct tcggcagcgt gatgtcgtga
    10741 atgtccccga tgaacagcgc attgccgaaa ccgattgcct gcccgctctg cgccgtgtag
    10801 atcccctgca cctgccaacc gccgaagaac ttgtccacga cgcccggcat gctcgagagg
    10861 tatctcttgc ctctgccaaa gggcagttcc cagatcgcgc tgacgaccac gcggtggggc
    10921 cggtcctggt ccgagacaac gtactcgagc ggcgagtacg gcccgttcaa ccggctgaga
    10981 gcctccatgt ttttcgacca cagatagttg gcggtaacgg tccacccact ggcaaaacgg
    11041 cgttcggtcc tcacttgcag cgcgtgatac caggagtatc cgtcgtactg cgttgtactg
    11101 atgtctgtga aatgcggata gtcgccggac gagagcaggt aagagcggga tgtgttcgcc
    11161 ccgttcaggc cggtgcccgg cagcagaccc gcgaaggggt tgggcacttg ctggctcagc
    11221 caattgatgt gcgccgtatc gcggtaaggc gaggtgctca agtactggag cggcaaccca
    11281 cgcagatctc tggtggtctg gatcgagctg gcgtggttgc cgacgtagcc gagttcgaga
    11341 agcacgcgct gcggcagttc gcgctggatg ctgagctgcc agcgctgttg ccgggatgcc
    11401 tcggggttct catcgaagaa ggtgatcgca cggccggtga aggtcatcag accgtcagcg
    11461 gcgccgcgcg gatcctcgat tccggtgggg tagggattgg cgagcgtggc cacgaacgtc
    11521 aaaccgctgt ccagtgaggg aaccaggttg gtggcgtagc tgaagccttc ctgcttcacg
    11581 tcgccgcgct gcaagccgag ctggccgaag tagacgccgt aaccgcctcg cagcacggtc
    11641 ctggggccaa tctggtaggc aaaaccgata cgcggcatga tgttgttctt gtcgcgggta
    11701 aacagcgtac gcggctgccc gttcacgccc gcaaacgtca cgccgcccat cagccggaac
    11761 tgctccggcg gcagctcggc aatggggttc ctggcgtagt tagcgcgcac cacatcgttg
    11821 agcgggttgg gcgtatcgaa gtcgtagccg cgaatcgatc gatcccagcg ttccgttagc
    11881 ggaccctcga tctcgtagcg aaggccggcg ttgatggtca ggctcggcgt gacacgccag
    11941 tcatcctgca tatagagcga ccacacggtg gattgttccg cgaagctcgc attgcgctcc
    12001 acgtacccgc cgctcggcag tcccagcatg agcgctgcgg cggcctggcc aatgggagcg
    12061 accggcgagt tgtcgaatgg gccgcgggtc catgtgttgt cgaagttgaa tctgccggta
    12121 gaggccctgt tccatacgta gtgattggtg cggtattgac ggccgtcgcc gccgaaacga
    12181 agggtgtgat ttccgcgtac ggcggtcagg tgcgcctcga ggctggcgtt cgccatcggg
    12241 tcgcggaacc aggtgttcgg cgtggcggtg tagccggtaa tcgtgatgta agggaacgcg
    12301 ctgtaggatc tatcgatggc gtcggtataa atcggcggaa aacccagcga agcgagatcg
    12361 aagcccttct cgttgagcgg cgtctgctgg atttgcaggc ggaaatagcc ggcacgcagg
    12421 ttgagcagcg tagtagggtt gatctgataa acgtgatcca gcgaagccgc gtgctgcttc
    12481 cactccgagt cggttccagt ggccgcgctg cggaaccagt cgctcgaata gctgaaccgc
    12541 cggtaggtat tcacacgggc gaacatgcgg tgcttgtcgt tgaaggcatg gtccacgcgc
    12601 gagatatggt tgtaataggt gatggcttcc gggtcgttga cgcggatcag gttattgagg
    12661 ccgtcggccg tcccgggggc gttcggcagc gaccagtagt tgaggatgct cttcgcaatc
    12721 gggctgatgc ggctcgaagg aacgatgttg ccgggaagcg gatccaccac ggtgcggccg
    12781 tttggtgctt gtttgcgggt ggccgggtca tagacctggt actgcgagcc gagcttgagc
    12841 agagcggaaa aatcgccttc ccgctgcgcg gcggtaggaa ccgtcaaggt gccggtgcca
    12901 taggccgaac ccatgggacg gctctcgtga atgccctcgt aaccgtaggt gaagaacgtg
    12961 cggtccttac cgttgtatac cttgggaatg tacaccggcc cggtaaggct cccgccccag
    13021 cggttgtagt cgaactcgcc gcgcggctgc cccgccttat tggcgaagaa cagattggcg
    13081 ttcagcgacg ggtcgagcac accccagtac gcggtgccgt gaagctggtt accgccggat
    13141 ttgagcgtga tgctggtgac gccgccctgc gtatggccga ccgacgcgtc gaagggcatc
    13201 gtttgaatct tgaactcctg aacgatgtcg gcaggcggcg tgtagccggc gatggtatcg
    13261 gtggtgccgc gtttgacggt ggaattctgc acgccgtcca tggtgatctc gctcttgttg
    13321 gccttcacgc catccatgga gtagcctacg atgtgcgtgg gttcgaaggg acggtcgagc
    13381 ccggcgttct gggtgtatac cacacccgcg ctgagttgca tcagcagata cggattgccg
    13441 tgggcgatgg gcagttccgc gatgcggcgc tggtcaatca cttggcccat ggaagcattg
    13501 gtcgaatcca gcagcggggt ttcggcagta acggtcaaaa cctcggcgag ttccccgatc
    13561 tgcaactgga tcgggatatt caaacgatca tcaatgcgga tttcaatatt gtcgcgaatg
    13621 aaggtcttaa acccgcccac ttccgccttt aaaacgtaga tacccgacac aagatagggg
    13681 atgccgtaat tgccttcggc attggagcgg gtggaaacgg taacatttgt tgctttattt
    13741 gttgcatgaa cgaccgtgcc cgggattacc gcgccactgg tgtccgtgac gcgtccctgg
    13801 atcgacccac gggtttcctg tgccggcaat gcctggcacg aggctaaacc gagtaagaca
    13861 actaaccatt gaatcctcat ggaatggact ttatcagata cgattatccg gaacaatcgg
    13921 cgcccattct gtttccgtct tcgtcggccc gccggactgc gtgtgggact tttcgcgccg
    13981 ttatgccagt gtggatcgat tttccggccg ctagaataaa ggtgtgtccg aagccgatat
    14041 ccaaatcgtg cccgatgagg ccggccagcc tacggctgtc atcgtgccca ttgcgctctg
    14101 gaaagagatc gtttccgaac gcgaaaccgc atacctgttg aagtccgaga ctatgcggca
    14161 acggttgctg gcggcgctgg aacgtgggaa cggagtgccg ctggatgacg ccgtgacgaa
    14221 actgggcttg tgagaagcgg cctatgcctg atgtcgggtg cgccaaaact tagcgacggc
    14281 ccccgccatc tccagtgaac ctacaacaca gcaaatgcgt atggccgcta taacgctcct
    14341 tcgcgtccta tgccagtatg aaatggatat gttggatgct gcatcgaagt ttcgtgtcgg
    14401 gctgatacag atgtcctgct ccgcgaagcc ggaagagaat ctcgagaaag cacagtggaa
    14461 gatccgggaa gctgcgggca aaggcgcaca gatcatctgc cttccggagc ttttccgctc
    14521 tcagtacttc tgccgcgagg aggacgcggc gcttttcgcg ctggcggaaa cgatcccggg
    14581 tccatcaacc gatgtgctaa ccagactcgc ggccgagttg aaggtggtga ttgtcgcctc
    14641 actgttcgaa aaacgcgcgc aggggctgta tcacaatacg gcggccatca tcggggccca
    14701 cgggaacatc cagggcatct accggaaaat gcacatcccc gacgatccgc tctatttcga
    14761 aaaattctac ttcacgcccg gcgatctcgg cttcccgaac ttcgacaccg ctttcggaaa
    14821 gatcggtgta ctggtctgct gggaccaatg gtatccggag ggagcgcgcc tctcgagcct
    14881 ggcgggcgcg aacatcctgt tctatcccac ggccatcgcc tggcacccct ccgaaaagga
    14941 gcagtacggt gcggcgcagc tcgacgcctg gacgaccatc cagcgcgcac atgccatcgc
    15001 caacggtatt tatgtcgcgg cggtcaatcg catcggcttc gaagggccct ccgatcgcgg
    15061 cctcgaattc tggggctcct ctttcgtcgc cgatcctttc gggcaagtga tcgcccaagc
    15121 cccggtggat cgggaggaga cgctggtggc ggagtgcgat cccagaaaga tcgatgaagt
    15181 ccgccgcaac tggccgttcc ttcgcgaccg ccggattgac gcttacggtc cgctcgtgta
    15241 ccgatggctc ggctgaacca acgtacaccc gcggcgctcg gataccggat gcccgccgag
    15301 tgggagccgc acgaggctac ctggatcgca tggcctcatg aacgctccga ctggcccggt
    15361 aagtttgccc ccgtgccctg gatttactgc gacatcgtgc ggcacctggc ccgcctggaa
    15421 aaggtccgca tcctcattgc atcggaggaa gcggaacggc aggcgtcccg tatgctcgaa
    15481 aagtccggag cagacctgga tgccgtagag tttctacgct tcgagacgga tcgtagctgg
    15541 tgccgggact actgcccgat ctttgtgaag aaccgtgccg gagacgtcgc catcacggac
    15601 tggcacttca acggctgggc caagtatgag aactggacgc gcgacgacaa agcacccgcg
    15661 gccatcgcga aaaaactcgg catgcccctc ttccggccgg tttcgaatgg ccggcgggtt
    15721 gtgctggaag gcggaagcat cgatgtgaac ggtgccggcc tgctgctgac aacggaagag
    15781 tgcctgctga gcgaagtcca gagccggaac ccgggcaccg gacgcgccgg gatggaacgg
    15841 attctggcgg atcacctggg tattcacaaa gtcctttggc tcaaaaacgg catcgccggg
    15901 gacgataccc atggccatgt cgacgatatc gcacgcttcg tgaatcccac gacagtagtc
    15961 gccgcatccg aagacaaccg tgaagacacc aactacgcaa accttcgcga gaatctcgaa
    16021 ctccttcgcg gcatgaaaga cgaagaggga tgtccgctga aaattgtaaa gctacccatg
    16081 ccggcgccgg tgtggttcga cggacagcga ttgcccgcga gttacgccaa tttctacatc
    16141 gcgaacggaa ttgtactggt gccggtgttc aacgatccta acgaccgtat cgcgctgaat
    16201 acgctggcgc ggctgtttcc ggatcgtgag gtaacgggca tctattcccg cgatctggtc
    16261 ctcgggttgg gaaccctgca ctgcctgaca cagcagcagc cggccgccgg tgcgctatct
    16321 tgataaagtt ttcctctgcg ctcgtctaat aacaatagtg cagaatgata gctgaagtca
    16381 gttcacacta ctggaggcga aacaatgaag tattgggcac aactcacagg cacaggtctc
    16441 tttgcactca cgatggcatg cggacccagt acgccgcccg caaccgaaac caaggaggag
    16501 gcaccggccg ccatgctaac tgcaaaagca gagctcagat cgtcgaaagg cgaggacgtc
    16561 ggacaggcaa ccttcactca gaccaacggc tccgtgcgtt tgtcggtgga ggtgaagaac
    16621 atgccgcccg gcacgcatgc gatccacgtc cacggagtcg ggcagtgcga cgcacccgat
    16681 ttcaagacgg cgggcggcca cttcaacccc ggcgcaaagc agcacggttt tgagaatcca
    16741 cagggccatc acgtaggcga catgccgaac atgaccgtcg gtcaggatgg aaccgggact
    16801 ttcgaataca ctattgaggg cgcgtcgctc gccgcaacgg gagagaattc gctgttccat
    16861 ccgggcggga cggcgctggt tgtacacgcc gacccggacg acatgaaaac cgatccggcc
    16921 ggcaatgcgg gcggccgcat cgcttgcggg gttgtcaccc ggtaagcagg ttcggaaacg
    16981 gaatccatat ggagcttgag ccgtgcactg gatctggaat ctgggaggac ttacatggcg
    17041 tgaacttgcg cgccgtgtgt acggccagat cctgaaggac gatgttttgg gccgcgcggc
    17101 tcagctctcc tactactttc tcctcgcgct gttcccgctg atggtgtttc tgatggtggt
    17161 gctcggctac ctggccgaca ccggaacatg gatgcgcgag cgtatgctgg aatacctcgc
    17221 gcacgtcgtt cccccttctg catttacact gattctggac accctggatg ctatcagcaa
    17281 cggcggcgga agcggcaaac tttcgctcgg tctgctggga gccatatggg cggcgtcggc
    17341 gggaatgaac gccgtgattc gcggactgaa catcgcctac ggtgtaaaag aagcacgcgt
    17401 gtggtggcgc gcgcggctgg tggccatcac gttgacgctg gcgctttcca tctcttccct
    17461 gttcaccttg accctgtttc tatggggcga ccggatcggc agtttcgtag cggagtggct
    17521 cgggcacacc gcattgttcc agacgatatg gagttgggca cagcccgtgc tggtggtcgc
    17581 tttcctgatg gcgaccttct ccgccatcta ccgttttgcg ccgaacctgg aggaacagaa
    17641 atgggtggcc acgcttcccg gcgcggtgct cggcgtcacc atctggctgc tcgcctcgta
    17701 tggactgcgg ctctacctgg tttatttcac caactacaac aagacctacg gctcggtagg
    17761 tgcggtgatc atcctgatgc tctggtttta cgtcaccagt cttgcgttgc tgataggcgg
    17821 cgaaatcaac tcggaaatcg agaatgccgc cgccgaacgg cgtgagcccg gcgcgaagtt
    17881 tttcggcgag cgtatgccac acgaacacgc ctggcgcggt tgatttaccg gacgaactgc
    17941 ggaggcgacg ggaaataggg atcctgctgc gggatggcgg cgccatcttt ccgcggcgat
    18001 gacgcaccga attttacctg agtgccggaa tcgtaaatga cggcctgccc gccgcccact
    18061 cttcccgagt aagccgccct cacttcaaac tggtggccgc gcttcgacaa ctcatcccgg
    18121 acttcctggg gaaccctgtt ctccaccatc acatcgcagc ccccgaacgt gagcttcgtg
    18181 aaccgcggcg cttcgagcgc ctcctggata ttcatccccg catccacgac attcgatacg
    18241 aactgcgcat gggcctgcgc ctggttgtat ccgcccataa tgccgaaacc gatgtgtgtg
    18301 ttgccctttt ccatgtagcc gggaatgatg gtgtggaagg gccgcttgtg tccggcaagc
    18361 acgttgggat ggccgtcctc gagcacgaac aggccaccgc ggttgtgcag atggaagccc
    18421 atgccctcca ccatcacgcc cgatccgaat gccaggtaga tgctctggat cagcgatacg
    18481 atattccctt cccggtctac caccgacaga taggtcgtgt cgccgttcgt ggagggagca
    18541 tttccgggct tcacctcgca cgatgccttt tccattcgga tgagcgcggc gcgctgttcc
    18601 gcgtagggct tcgagataag gccccccacg ggaatgcacg cggtacgggg atcggcgaca
    18661 tatttcacca gatccatgta ggccagcttc tgggcttcga tctttacatg caaggcatcg
    18721 gcggcatcca tctttgccaa ggggaaccgc tcgaagatgt tcagcatttc aagaaccgcc
    18781 agtccctgac cgtttggcgg aagctcgtag accttccatc cgcggtagtc cgtcatcacc
    18841 ggctccaccc attcggactg aaaatccgca agatcggcgg cattcatcac gccgcccagc
    18901 cggtcggacg tttgcaggat cgccttcgca atcggtcccc ggtagaaagc gtccgcgccg
    18961 ctgtccgaga ccagcttcaa cgctttcgca agctgcacgt tgcggaaaat ctctccaacg
    19021 gcgggcggct ttccgtcttt caaaaacagg cggcgcgcgt tctcgtcgct cgaaagcttc
    19081 tgcatgccgt cctgccagtg cccttgaatg atttccgtaa ccgggaatcc gttctcggca
    19141 tagtaaatgg cggggcggaa caactcggac cacgcgagcc ggccgaatct cttgtgcagc
    19201 ttctcccatc catcgacaca acccggaacc gtgacggaat tcatgcccag ctcgggcatt
    19261 tttgcgatgc ccttgctctt caggtactcc accgtgagcg ctttcggagc ccatccactg
    19321 gcgttcaagc catacagctt tccggtcttc gcctcgcgat agatgacaaa aagatccccg
    19381 cccataccgt tcatcatcgg ctcgacgaca ctgagcgcgg cattcgccgc aatcgcggca
    19441 tccacagcgg aaccgccgcg cgccagcacc tgggcgcccg cctgcgatgc cagcgtctgg
    19501 gcagtggcta cgatgccctg ggtagtgacc accatggagc gggcggtcga acgttcctgc
    19561 gctccaaccg aagaaacaag gacgagacac atgagtaagt acttcgacat gttgtgaaga
    19621 ttattttgtc acaggcccgc gcgtaggcgg taacagagcc gcgacggtaa gaagccgtga
    19681 agaaattgaa tttagccacg gatgaacacg gatgaacaca gataagtttc ctgttttcaa
    19741 tctgtgttta tccgtgtcca tctgtggctc tgaatctttc acaccttcta acggagcggt
    19801 agacccgggt tcgcaaagca tgaaatccct cccaaccgct cgcttacgct cacggctcgg
    19861 ctacaagctg tggcttaggt ttatcccgtt tcatatcgcg cttccgcgcg ttcaggcctt
    19921 cttgcggatc tcaatattca ggcgcttgat cttctggttc agcgtggaca acgggatatg
    19981 gagctgctcg gcggcttccg tctggctcca gttcacacct tccagcgctt caataatctt
    20041 gcgccgctca aagtccgcca caatctcgaa caggctcgcg tcgggcggaa ggtagccctt
    20101 gtcgtcgcgc cggatgcgaa taccaccggc ctgcaacaac tggtccggca agacatcgac
    20161 ggtgacggac ggcccggaag ccagcaccac cgcgcgctcg acggcatttt ccagttcgcg
    20221 aacgttcccc ggccagttgt aatccatcag gatctgcatg gcgtccggct caaattcgag
    20281 caccgagtgt cccacggaat cgagaaaacg ctcgttctcc cggcagtacc ggtcgaagaa
    20341 atgccggatg agcaagggaa tgtcttcctt gcggtcgcgc agcggcggca ggggaacgtt
    20401 gatcacgttc agccggtagt acaggtcctc ccggaatcgt ccctcctcca ccaatctctt
    20461 taaatccgcg ttggtcgcgg cgatgatccg gacatcgaca cgcacaatct cggtagatcc
    20521 cagcggccgg aactcccgct cctggatgac gcgaagcagt ttcgtttgcg tctccaggct
    20581 gatggtgccg atttcgtcaa agaagatcgt gcctcgattg gcgatctcga agtacccttt
    20641 ttgcgacgtt accgcgctcg tgaaggcgcc tttcacgtgg ccgaacaagg tggattcgag
    20701 cagatcggga ggcagcccgc cgctgttcac cgcgaagaac atgttatcgg cgcgcggcga
    20761 gtttgcatga atcgccttgg cgatcagttc cttccccgtg ccggtctccc cggtgatcag
    20821 aaccgtggtc cggctgggcg ccacttgcgt gaccaggtcg agcacgcgca gcatgcggtc
    20881 gctcttgcca acaatgttgg ggaagctgta ccgttgcttc aacacccgct tgagctgggt
    20941 attttcccgt tccagccgcc cgcgagcgat catcccctcg atttcaatca gcagcttctc
    21001 gttatcccag ggcttcggga aataatcgtt cgcaccggat ttgatggcgc tgacggccac
    21061 ctcgatcgag ccataggccg tgatcatgaa gatcggcgtc ccggtatcgc gttcccggac
    21121 ctccttcagc acctccatgc cggagcggtc cggcatcatc aggtccagca acaccaggtc
    21181 gtaggcggta ttctccatct tccggaggcc atcggtcccg ttgacggcta agtccacctg
    21241 gtagttttcg agcgacagca gcgtctccag gctctcgcga atatcggctt cgtcgtcgac
    21301 taccagaatg cggcctttat gtttgggagg agagagaacg tcgagcgccg tgtgttcaga
    21361 catttaccgg cttcctcgaa agagggaatt cgaggtggaa gcgcgtaccg ataccaactt
    21421 cactctggac ctcaatggag ccgccatgct ctttcacaat accgtaggtg accgataaac
    21481 cgaggccggt tcctttgcgc gaggccttcg tcgtgaaaaa aggatcataa atgcgcgcca
    21541 ggttctcgga ggcaattccc tggccggtat cggaaacatc gacgcgggcc agtccccgat
    21601 cgctacagct ccggatgtag agcacgcctc cggaattcat ggcgtcgcgc gcattcagaa
    21661 ccaggttcag aaacacctgc tgaagttttc cggtgttgcc gcgaatcgcg ggcatcccgt
    21721 cctccagatg caggctcacg ctgacgcccg ctttctggag ttgatgttcc agcaaggcca
    21781 cggtttcccg cagcaccttg ttcagatcca catcgtcata ggaggtggtg gaggtgcgcg
    21841 agaaattcag gagcgagttg acgatctcgc tcgcccggaa cgtctgcttg gcgatcttgt
    21901 cgagcagctt cgatttctga tcgtcgcccg gaatctgctt ggccagcatt tgagcgtagg
    21961 tcgagatgac cgccagcggc gtattcacct cgtgcgcgac cccggcggcc agcaggccga
    22021 tcgagctgag cttgtcggcc tgtaccagct tctgctccag ctccgtccgg tccgtgatgt
    22081 cgtcgaagat gatcaggcgg ccgatctgct ccaggtcgcg cgaaaccagg ggagcgacgg
    22141 cgatgttcag gatggcgccg ccggcggcct cggccgcgcc gtccggtgct acttcatgcg
    22201 taccggagcc gttgccgtta gtacccccac ccgcatatgc accggcggca agctccgcaa
    22261 cctgcgccgg cagggaatgt gcccgcaacg cgaacttgta gatgtgatga atgcccgttt
    22321 cgccgcggac gctatcgaac cggtcgcaga ggtccctcgg aatcaactct gtcagcgtgc
    22381 ggcccacggc gtactcgcgc ggaatccccg taagcctctc catctgggta ttccagcttt
    22441 ccacccggtc cgccaggtcg gcggcgagga ttccgacgtt tatggattcg acgatgttct
    22501 cgctgaactc cttcaggcgc tcatactcat ccactttgcg ctgcaaagtc cggtacagcc
    22561 gcgcattttc aacggcgatt ccgacataac ccgaaatcgt ttgcagcagc tcgatgtcgt
    22621 cgctgggcag aaagtcgtct tcttccgtcc ggctcgctcc gatataggcg atggtgcggc
    22681 cccgaacctg gcacggcaga tagtaagtga ggtccaggtt ggcgatcgtg tctcgaaccg
    22741 atgccggcca gtgggtggaa acgacatcca gcgcgtgccg cgtgcgttcg aagaacaggt
    22801 agggcttgcc ggactccgcc gacaaaaaac tcaggtccag atcgccggtg gaaagctgcc
    22861 cgttgcgcgg caggcggccg gtgttcatct tcaggtagaa acgcccatcc tcctcgccga
    22921 gcaggaagaa ggcgatgtat tgaatcgaga gcgtccgccg cagccggtcg gctaccgagt
    22981 tcaaggtggc atccaggtcg gtttccgagc cgagctcgcg ggcgaactcg atcagggtgc
    23041 ggcggtagtc gtagcggtct ttgtagaaga tatagcggtc gaactgttcc tgtagccatt
    23101 tccggatggg ctgaaagatg aaggtcgcaa tcagcagcag ggaaaccagg gcggtggagt
    23161 tcaattcctc catacggccc cacacggaaa gaatcagcgc ataaaaaatc gcgatcacgg
    23221 acagggttgc tgccgtgtag acatagccct gctggaaaat gacatccaca tccatcagcc
    23281 ggtagcgcag gatggcgtag gcccaggtga ggggcaccag cagaagcgaa aacaccattg
    23341 cgtccattac ccttccgggc actgcgccga ggacatatgg caggacgtag aagagcgtga
    23401 agggaacgac tccgaacagc gcgccgttgc gcagccattt gagctgctgg cgcacgatcg
    23461 gatcttcccg gtgggaatga tactgaaagg tcagcacgag ggcgcccgcc agatagagtc
    23521 ccgccatgag cagcagccac aagcggtcga gcatccaacg gacttctaca agggacacgg
    23581 cgatgcggat ggtcccggag gaaaagccta cgtaagcggc aaccagcaga aggcccggca
    23641 cgtacagcag agcgattcgc cagcggctcc gaaaccactt cccccgttcc gggaacgtga
    23701 gacagaaatg tagaaaaatc gtgggcgcca gcacccccgc cgcgacgttg ccccagtaaa
    23761 tcaccttgtc gaaattgttt aatttgccgg tgtaatgaaa ggcgaaaaga acgaaagagg
    23821 ccaggcagag aatataaaag tgccgtgctt ttggagcgcc gccccggcgg aaatacacga
    23881 acagtccgat ggagaggtat gccagcgcga caagatactg gtagtaggtg gcaccctgcg
    23941 gttcgcgctc tcccaaaatg atggggacct tgacctccac cccctgccgg tggataaggt
    24001 agtccgccga gcgccaccct ccgattccca ccaggatcct ggtgacatcg agcgcgttcc
    24061 ggaccggggc gccattgatc gcgacaatga tatcgcccgc ccgtaggccg cggttatccg
    24121 cctggctgcc gggaacgatg tggagcgcct ccacccggcc gttccggtcc acccagatgg
    24181 ctccgtcgtc cggtaacttt acctttttct gttgttgaaa attcagggcg gcggaaatga
    24241 cagcggcggc cgttagtacg accagaagtg cgttgacgag ttggtccttt aactccctca
    24301 ccgccgaagt ccgcccaccg tttataggta caactattgt gccatggaat gcgttgcacg
    24361 tctgctgcca cggcagtgca ctgcccttaa cagaagaata cagccgtttc gcggtggaga
    24421 caaacccgct acccggacgc gaattcggtt tttgggatat acactatgcg gaagtgtagg
    24481 gattgccgtt gcctgggcat tgtccagggc acggtagaat taaaggaatg caccggcttt
    24541 tcatgcttct tctgctcagc tttccggctt ttggccagtt tgtatttggc gtaaaaggcg
    24601 gcgtacctct gagtgacgcg ttcgacatcg cgcgagcccc gagtccggcc agggaattct
    24661 cctcggctac gcgccggtac accattggac ccacggccga acttcgtctc cccttcggtc
    24721 taggcatcga attcgacgcc ctgtacaaac gcctgggcta ctcttcgagc ggaagtaccg
    24781 cgaacctcac gttccattcc gaaacgaccg ccaactcctg ggagttcccc ttgctggtga
    24841 aatatcgcgc accgggagcg ctggcccggc cgttcgtgtt aggaggcgtc tccttccatc
    24901 gcctgagtga cgtgaagcag ttcaccgtgg agacttttca aacggttacc ggcgaccgga
    24961 cgtcaaccac cagcgagccg gaggaattga gggataagtc cgaccagggt ttcgtcctgg
    25021 gcgcgggtct ggagctgcgt gcgccagtga tccgcatctc gcccgaaatc cgttataccc
    25081 gctggggaag cgagaacttc ctggacgcgc tgaacattct ccgctcgaac aaaaaccaat
    25141 gggagttttt aatcggtttg acattttagc cgggccttca atccggtata atttatccgg
    25201 ttgagttcac cacaacagga tgtcacccgg ctgctcgtca actggagcaa gggcgatcag
    25261 caggcgctcg aggacctgac gcctctcgtt tacggcgaac tgcggcgtct ggccggccgg
    25321 tacctgcgcc gggaacgctc cgatcatact ttgcagagca ccgcactggt tcacgaagct
    25381 tatctccggc tggtggacca gcgtaacgtg aagtggcaga accgcgccca ttttttcggt
    25441 gtcgccgccc aactgatacg ccgcatcctc gtcgactacg cgcgcagcca tcagacgctg
    25501 aaacgtgggg cgggcgcctg taagctctcc cttgacgagg ccatcggcgc acccgaaaag
    25561 cgggacctca acgtggtggc gctcgacgat gccctggacg accttgctaa aatcgacccg
    25621 caacaaagtc ggatcgtcga gctgcgcttt ttcgcggggc tgtcgattga agagaccgct
    25681 gaagtcctca agatttcccc ggccactgta aagcgcgact gggtggtcgc gaaggcatgg
    25741 ctctatcgga acatcagcgg gagggagtcg ggtgacacct gagcgatggc ggcaggttaa
    25801 agagattctc gccacttcgc ttgaactgga acccgaaacc cggaatacct atctcgatac
    25861 ggtgtgcggc gccgaccccg gtatgcgagc cgaagtggaa tcgttgatcg ccgcttacga
    25921 ggaagccggc gatctgatgg aatctcccgc cggcgggaaa aagttcagcc tttcttcctc
    25981 gtctattgaa accgacccgc tccgcaggat tggtccttac ttcatcgtcg agcgtatcgc
    26041 gcagggcggc atgggcgcgg tgtaccgctg ttaccgcgaa gacgatcccg atcgcaaaac
    26101 ggtggccgtg aaggtactga agccgggcgc cgacagcagt ttcgtgctcc gccgctttag
    26161 ccatgagcgc gaaattgtag cgaatctcca tcacccgaat atcgccaggt tcctggacgg
    26221 cggcagtacc gacgatggat
//
LOCUS       DASTMK010000306         7100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_495662, whole genome shotgun sequence.
ACCESSION   DASTMK010000306 DASTMK010000000
VERSION     DASTMK010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495662"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFQ79_13430"
     CDS             <1..580
                     /locus_tag="VFQ79_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140717.1"
                     /translation="AGIGSLERLLLGYSDVSDEGLAHLSGLPRLSILELSGTDAGDEG
                     LRHIANIQSLRDLALNSCRFTNKGLAALSGLAGLERLELSRTRTGNEGIVALAQLQNL
                     ESLNLDYTAIDDKALEVLRTLPKLRELTLDTADVTDAGIASLKSIPSLKVLNLYHTLV
                     TEKGMAELKAALPDCAIIFDRESALPSRRGKS"
     gene            715..1620
                     /locus_tag="VFQ79_13435"
     CDS             715..1620
                     /locus_tag="VFQ79_13435"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140718.1"
                     /translation="MKTILTCVFAVTLLAAEPAGWIEDLGGSATRDSSERLVAVDLRA
                     SWVSDADLRELAGEPNLERVDLSLTRISDRGLQQLKGAPRIAELILYYAEQITDEGLA
                     AVKGWKQLRRLDLRGTKITDTTIDHLSGVPSLESLDIGFAQVTDSGLDHLVALTNLKE
                     LTIGGNKLTDVGVQALRQLPGLTYLDVSGAQRTDSGLWSVSLTEAGVDAIGTLKELRH
                     LRLNGTNVSARALEKLKNLTSLERLDLEDCKRVGDDAVSVLASLPTLRRVELSGTGIS
                     GKGLTELRRLRPSIQVSSTRGSLLD"
     gene            1613..2821
                     /gene="ilvA"
                     /locus_tag="VFQ79_13440"
     CDS             1613..2821
                     /gene="ilvA"
                     /locus_tag="VFQ79_13440"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEU0140719.1"
                     /translation="MIDFHAIEAAAGRIKPEIYESPLVHTKTLSRLTGNEIYLKLENL
                     QMTGSFKERGALNRILTMTAEEKRRGVIAASAGNHGQAVAYHAALRGIPVQIWMPRYT
                     PLIKLDATREYGADVVLHGNSYDEAYEAALQRSLETNATFLHAFDDEGVIAGQGTIGL
                     EMLAQNPHLEVVVTPIGGGGLIGGIACAIKGRNPNVEVIGVQTSRLPSMKAALEHRQP
                     VSVPAASTMADGIAVRRAGKLTLPLVSEYVDRIVTVDEEEIAEAVLVLLEREKTLAEG
                     AGAVALAAVLQGKTGHSGKKIAVLISGGNLDVNLLSRIIEQGMVRDGRRLRVRVRLPD
                     YPGALEGLSSVIARMGANIVETFHNRAHYGVSLSETAIDITMETRGRGHVTELLAELK
                     KSGYEFAIVQ"
     gene            complement(2836..4182)
                     /locus_tag="VFQ79_13445"
     CDS             complement(2836..4182)
                     /locus_tag="VFQ79_13445"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEU0140720.1"
                     /translation="MPQVITRRGRLSRRRFLRGLTLSGSAVSVGLPPLVSMFNTNGTA
                     YAAAPDAAPTVPEAPIENRFVLWFNGNGIPERYWIPAETGSDFRLTPCLSPLAPFRED
                     IHVISGLDNSAANLPGPGNGHHNSMSGLMTCTMYTGRGAGGPSIDQAIAANIGGESRF
                     RSIQIGVSQESFGESIQRNMSWSGYDRSLPPEMLPDRLFDRLFGRREEGWVNRKRSIL
                     DAVQEDAATLTKRLPTADRIRVEEHLSAVRDLERAIAGLPPEYRRIDPPEIEDMKDWP
                     RVAKVQSDLLAHALATRQTRVASYMLTKCQGLSRFPWLGYTAARHHDYTHSDGKAPGA
                     EGVSGQRILRDICRWHVEEFAYLLARLKSIPEGDGTVLDHCCLVYVHEHAEANPHKNS
                     GLGLIVAGHAGRLATGMHTKSTGTIGDLYLTLADEVMSASLGKFPSATRQLTEVLT"
     gene            complement(4154..6124)
                     /locus_tag="VFQ79_13450"
     CDS             complement(4154..6124)
                     /locus_tag="VFQ79_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEU0140721.1"
                     /translation="MRFALFLAVSLTAPAIASADSFSEDVYPVLDKAGCAGCHNSGGV
                     ASATKLHFPERDAPAARIESFGRSLVVLVNRERPEESLLLTKPTNRMRHTGGERIKPG
                     SPQEATLRKWVQVLANLSPEEVAAAKKESEEHKSGRGTKAPGVVLRRLTHSQYNNTVR
                     DLLGDQTAPANRFPPEDFVNGFKNQYESQNVSPLQVEAYSLAAERLARSAFRGGDTRG
                     LILCKPSAECRSRFIRDFGLRAFRRPLDDTEQKRYEQLFALETDFLAGAQLVLEAMLQ
                     STSFVFRLDQTSNPAWQPYATASRLSYALWDTMPDEALMNAAARGELSTPQGIEKTAR
                     RMIEDPRAREALNEFVSQWLRFDRVLTTARNRRRYPRFNRESAFAMTEEARQFIGDLV
                     WNDRDFMEAFTAPYGFVNADLAGIYGVPAPEKAFERVPLPSESERAGLLGQALFLALT
                     AKPDDTSPTARGLFVREQFLCQHVPPPPPGVDTNLPPVSEARPMTNRERMQRHVTDAS
                     CSACHNLVDPIGFGFEKFDAIGARREMAKLFFADNEHDRRAPPKVVELALDTSGSLAG
                     VPDSNFTSPRELGAVLAKTPQCQECVVKQYFRYTVGRMETLADRPVIQRVFKDFRDSQ
                     FRFKELIISLVRTREFPDLGGRMHAASNHQAR"
     gene            6288..>7100
                     /locus_tag="VFQ79_13455"
     CDS             6288..>7100
                     /locus_tag="VFQ79_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0140722.1"
                     /translation="MQPLSRRHLLSAAAGIAGFAAVPASGKVERLTITGVDLHRVVVP
                     MKEDSTTDPETAESSRFDLVPKIILEVHTDSGITGIGETGRGEDYAAVQKNATFLKGR
                     KALDFNLTRLDLPARAGYAAFEMALYDIVGKAFGWPVYKLLGGLAQRKVLVGYWTGRR
                     TPKGMIRVAERAVAGKFTSVKTKCAQGDPVVETAEVLAKHAPGIKYIVDPNTRYKSYA
                     DFLPVAKALDAIGNALVFEDPFDKNDFEGYRKLRREVKTHVALHLGDPKAMIR"
BASE COUNT         1468 a         2029 c         2239 g         1364 t
ORIGIN      
        1 cgccggcatc ggcagcctgg agaggcttct gcttggctac tcggacgttt ccgatgaagg
       61 cctggcacat ctcagcgggc tgccgcgcct cagcattctg gagttgagcg ggacggatgc
      121 cggggacgaa gggctgcggc atattgccaa catccaatcg cttcgcgatc tggcactcaa
      181 ttcctgccgg ttcaccaata agggccttgc cgcgcttagc ggcctcgcag gactggaacg
      241 gctcgaattg agcagaaccc gaaccggcaa cgagggcatt gtggcgctgg cgcaattaca
      301 gaatctcgaa agtctgaacc tggattacac tgccatcgac gacaaagctc tggaagtgct
      361 tcgtacgttg ccgaaactgc gtgagttgac gctcgacacg gcggatgtca cagacgcggg
      421 catcgcttcg ttgaaatcga taccgagcct gaaagtgttg aacctttacc acacgctggt
      481 cactgaaaaa ggtatggccg agctgaaagc ggcgctgccg gattgcgcca ttatctttga
      541 ccgcgaatcc gcgctgccaa gccgcagggg gaagagttaa gcacgacgtc ttaaatgttt
      601 gcgcgacgat ttgattggcc acggatggac acagatgaac acagataaga aaaacgcaag
      661 gcttgatccg tgttcatccg tggccgaatt tgcttttctg gattgctgtt tagcatgaaa
      721 accatcctga cgtgtgtttt cgctgtcacg ctgcttgccg ccgaacccgc cggatggatc
      781 gaagacctgg gcggctcggc aactcgtgat tccagcgagc ggctggtcgc cgtggatctt
      841 cgcgcgagtt gggtctcgga tgccgacctg agagagctcg cgggtgagcc aaacctggag
      901 cgtgtcgatc tatcgcttac gcgcatcagc gaccgcggat tacaacagtt gaagggtgcg
      961 ccgcgtatcg cggagctgat cctttattac gccgagcaaa ttacggatga aggtttggcc
     1021 gccgtaaaag gctggaagca gttgaggcgt ctggacctgc ggggcaccaa gatcaccgat
     1081 acgacgatcg atcacctttc tggcgttccg tcgctggaat ccctggacat cggcttcgcg
     1141 caggtaacgg atagcggatt ggaccacctg gttgcgctca caaatttgaa agagctgacg
     1201 attggcggca ataagctcac cgacgttggc gttcaggcgc tgcgccagct tcccgggctc
     1261 acttatctcg acgtgagtgg cgcacagcgg accgattccg ggttatggtc cgtgagcttg
     1321 acggaggcgg gcgtggatgc gatcggcacc ctcaaggagc tccgccacct acggctcaac
     1381 gggaccaacg tctcggcccg cgcactagaa aaactgaaga atctgaccag cctggagcgc
     1441 ctcgatctcg aagactgcaa gcgcgtgggc gatgatgccg tgagcgtttt agcatcgctt
     1501 cccaccttgc gtcgggttga gctgagcggc acgggcatca gcgggaaagg tctgacggaa
     1561 ttgcggcgtc tcaggccgtc aatacaggta tcctcaacaa gaggcagcct gcttgattga
     1621 tttccacgcg atagaagccg cggccgggcg gatcaaaccg gaaatttacg aatcccctct
     1681 cgtacacacc aagaccctct cccgtctcac cggcaacgag atttatctca agctcgaaaa
     1741 cctgcaaatg acgggatcgt tcaaggaacg cggcgcgttg aaccggatcc tcacgatgac
     1801 cgctgaagag aaaaggcggg gagtgatcgc ggcctcggcc ggaaaccacg ggcaggcggt
     1861 tgcttaccat gcggccctgc gcggcatccc ggtacaaatc tggatgccgc gatatacacc
     1921 gctgatcaag ttggatgcca cccgcgagta tggtgcggat gttgtcctgc acggcaatag
     1981 ttatgacgaa gcttatgagg ccgccctaca gagaagcctc gaaacaaacg ctacgttcct
     2041 ccacgccttc gacgatgaag gcgtgattgc cgggcaggga accatcggtt tggaaatgtt
     2101 ggcacagaac ccgcacctgg aagtagttgt gacgccgatt ggcggaggtg gtctcatcgg
     2161 gggaattgcc tgtgcgatca aaggccggaa tcccaatgtc gaagtgatcg gagtacaaac
     2221 gtcacgtctg ccatcgatga aagcggcact ggaacatcgg caaccggtga gcgtgccggc
     2281 cgcttctacc atggccgacg gaatcgcggt tcgaagggca gggaagctga ccttgcccct
     2341 cgtcagcgaa tacgtggacc gtatcgtcac cgtcgatgaa gaggagatcg cggaggcggt
     2401 cctggtttta ttggagcgcg agaaaacgtt ggcggaaggg gccggcgctg tcgcgcttgc
     2461 cgccgtgttg cagggaaaaa ccggccatag cggtaagaaa atcgcagtgc ttatttccgg
     2521 cggaaatctg gatgtgaatc tgctgagcag aatcatcgaa cagggcatgg tccgggatgg
     2581 gcggcgatta cgcgtccggg tgcggcttcc ggattatccg ggagcgctgg aaggactttc
     2641 atcggtgatt gcgagaatgg gcgccaacat cgtcgagacg tttcacaatc gggcgcatta
     2701 cggggtcagc ctgagcgaga ccgcgatcga catcaccatg gaaacgcgcg gcagggggca
     2761 cgtgacagaa ctgctggccg aattgaaaaa gtcgggctat gagttcgcca tcgtgcagtg
     2821 aacgtccggg acctgtcagg tcagcacttc ggtaagctgc cgtgtggcgg acggaaattt
     2881 gcccaagctg gcggacatca cttcgtccgc cagagtgagg tacaaatcgc cgatggtgcc
     2941 cgtactcttc gtgtgcattc cggtggcgag cctgccggca tgcccggcca cgatcaatcc
     3001 gaggccgctg ttcttgtgcg ggtttgcctc cgcgtgctcg tgcacgtaaa ccaggcagca
     3061 atggtccaac acggttccat cgccctccgg tatggatttc aaccgggcga gcaggtacgc
     3121 aaattcttcg acatgccacc ggcagatgtc gcgcagaatc cgctggcctg agacgccttc
     3181 cgcacccggc gctttgccgt cggaatgcgt gtagtcgtga tgtcgcgcgg cggtatagcc
     3241 caaccacgga aagcgtgaaa gcccctggca cttcgtcagc atgtaggagg caacacgcgt
     3301 ctggcgcgtg gccagcgcgt gtgcgagcag gtcgctttgc actttggcaa cgcgcggcca
     3361 gtccttcatg tcctcgattt ccggcggatc gatgcgccgg tactcaggcg gcagtccggc
     3421 gatggcgcgc tcgagatcgc gcacagccga caagtgttct tcaacccgta tccggtcggc
     3481 ggtagggagc cgcttcgtca gcgttgccgc atcttcctgc acggcatcca gaatgctgcg
     3541 ctttcgattg acccatcctt cttcacggcg gccgaacagg cggtcgaaga gacggtcggg
     3601 cagcatttcg ggcggaaggg agcggtcgta gccggaccag ctcatattgc gctgaatgct
     3661 ctcaccgaaa gattcctgcg atacgccgat ctggatggag cgaaaccgtg attctcctcc
     3721 gatgttggcg gcgatagcct ggtcgatgga cgggcctccc gcaccgcggc cggtgtacat
     3781 cgtgcaggtc atgaggccgc tcatggagtt gtggtgcccg ttgcccggcc cgggtagatt
     3841 ggcggcagag ttgtcgaggc cactgatgac gtggatgtct tcccggaatg gtgcgagcgg
     3901 gctcaggcag ggagtgagac ggaaatcgga gccggtctcg gcgggaatcc agtatcgttc
     3961 gggaatgccg ttgccgttaa accacaaaac gaagcggttc tcgatgggcg cctccggaac
     4021 cgtgggcgcc gcatccggcg cagcggcgta ggccgtaccg ttggtgttga acatcgaaac
     4081 cagcggtggc aggccgaccg agacggccga ccctgaaagc gtcagccccc ggagaaacct
     4141 cctgcgcgat agcctaccgc gcctggtgat tacttgcggc atgcatcctc cctcccagat
     4201 cggggaattc cctcgtacga actaaagaaa ttataagttc tttgaaacga aattgcgagt
     4261 cccgaaaatc tttgaaaacg cgctggatga caggccggtc ggccagggtt tccatgcgcc
     4321 ccaccgtata gcggaagtac tgcttgacaa cgcactcctg gcattgcggc gttttggcga
     4381 gcaccgcgcc cagctcgcgc ggggaagtga agttggagtc cggtacgccg gctaaggatc
     4441 ccgaagtatc gagggcaagc tccacaacct tgggcggagc gcggcggtcg tgttcgttat
     4501 ccgcgaagaa cagcttcgcc atttcccgcc gtgctccgat cgcgtcgaac ttttcaaagc
     4561 cgaagccgat agggtcaacc aggttgtgac aggcggagca ggaggcgtcc gtaacgtgcc
     4621 gctgcatgcg ctcgcgatta gtcatcggcc tggcttcgct gaccgggggc aggttcgtgt
     4681 cgacgccggg cggcgggggc ggaacgtgct ggcagagaaa ctgttcgcga acaaaaaggc
     4741 ctctcgccgt cggcgaagta tcgtccggct tggcggtgag agcgaggaac agagcctggc
     4801 cgagaagccc ggcgcgctcg gactcggaag ggagcgggac gcgctcgaaa gccttttccg
     4861 gagcgggaac gccgtaaatg ccggcgagat cggcattcac gaatccgtag ggtgcggtga
     4921 aagcttccat gaagtcgcga tcgttccaaa ccagatcgcc gataaactgg cgcgcctctt
     4981 ccgtcattgc gaaagcggat tcgcgattga accgcgggta ccggcgccgg ttgcgggcag
     5041 tcgtaagaac acgatcgaat cgtagccatt gtgaaacgaa ctcgttcaag gcctcgcgag
     5101 cacgtggatc ttcgatcatc ctgcgagccg tcttctcgat tccctgcggc gtcgataatt
     5161 caccgcgcgc ggcggcgttc atcagcgcct cgtccggcat cgtgtcccat agcgcatagg
     5221 agagccggct ggcggtcgcg tagggctgcc atgcgggatt ggacgtctgg tcgagccgga
     5281 atacgaaact ggtggattgg agcatggcct ccagaacaag ctgcgccccc gccaggaaat
     5341 ccgtctccag cgcaaacagt tgttcgtacc gtttctgttc ggtgtcgtcg agcggccgcc
     5401 ggaaagcccg caacccgaag tcgcgaatga accggctgcg gcattccgcg gaaggcttgc
     5461 agaggatgag cccacgcgta tcgccgccgc gaaacgcgct gcgggccagt ctttccgctg
     5521 cgaggctata ggcctccacc tggagcggcg agacattttg cgattcgtac tgattcttga
     5581 acccattgac aaaatcttcc ggagggaacc ggttcgccgg cgcggtctgg tctcccaaca
     5641 ggtcgcgtac cgtgttgttg tactggctat gcgtaagacg gcgcagcaca acgccgggcg
     5701 ctttcgtgcc acggccagac ttgtgctcct cggactcttt ctttgccgcc gctacttcct
     5761 ccggcgaaag attcgcgaga acctgcaccc atttccgaag ggttgcttcc tgcgggctgc
     5821 ccggtttgat gcgctcgcca ccggtatggc gcatacgatt cgtcggcttt gtcagtagga
     5881 gcgattcttc gggacgctca cgatttacaa gcacgacaag agaacggccg aagctttcga
     5941 tgcgcgcggc tggagcgtcg cgctcaggaa agtgaagctt ggtggccgat gcgactcccc
     6001 cgctgttatg gcatccggca cagccggcct tatccaacac cggatagaca tcttcggaga
     6061 acgaatcggc gctcgcgatg gccggagcgg tcagagaaac agcgaggaac agcgcaaagc
     6121 gcataagtga acccagaaag atagcgtggg ctccggaagt cccggtccgg agcgcccgaa
     6181 cccttacagc ttatcgcgtt tgctcccggc gggtttccgg acacgaatct ccttgacaaa
     6241 tccgtgcgcc gcgccggaca atcgacctca ataaagtaga gaccactatg caacctcttt
     6301 cgcgccgcca tcttctctcc gctgccgcgg gtatcgcggg tttcgccgcc gttccagcct
     6361 cgggtaaggt cgaacggctc accattacgg gtgtggacct gcatcgcgtg gtcgtgccca
     6421 tgaaagaaga cagcacgacg gacccggaga ccgcggaatc cagccgtttc gacctggtgc
     6481 cgaagatcat tctcgaagtt cacacagact ccggcatcac gggtattgga gaaaccgggc
     6541 gcggcgagga ttacgcggcc gtgcaaaaga acgccacgtt tctgaaagga aggaaggcgc
     6601 tcgatttcaa cctgactcgt ctggatctgc cggcgagggc cggctatgcc gcattcgaaa
     6661 tggcgctgta cgacattgtg ggcaaagctt tcggctggcc ggtatacaag ctgctgggcg
     6721 gtctggcgca gcgcaaagtg ctggtcggct attggacggg ccggcgtacg ccgaagggaa
     6781 tgatccgcgt cgccgaacgc gccgtcgccg gtaagttcac ctctgtaaag acgaagtgtg
     6841 cgcaaggcga cccggtggtg gaaacagcgg aggtgctggc caagcatgcg cccggcatca
     6901 agtacatcgt cgaccctaac acccggtaca aaagttacgc cgattttctt cccgttgcaa
     6961 aagcgctgga tgcgatcggt aatgcgctgg tgtttgagga cccgttcgac aagaacgact
     7021 ttgaaggcta ccgcaagctg cgccgcgaag tgaaaacgca tgtggcgctg cacctgggag
     7081 acccgaaggc gatgattcga
//
LOCUS       DASTMK010000307         9858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_527667, whole genome shotgun sequence.
ACCESSION   DASTMK010000307 DASTMK010000000
VERSION     DASTMK010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527667"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..821
                     /locus_tag="VFQ79_13460"
     CDS             207..821
                     /locus_tag="VFQ79_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140723.1"
                     /translation="MENSSPHTIARQLIQKAHNFDGLPEVVVGFFFLLVSALLYVLTV
                     LPKGTPGFQAAVLILAFGVPIFCFSARPLLNRVRRRYLVGREGYVRPKRAGRKEIATG
                     IVLAVLVAAVLFVLVPRLSHPDRWLLAGTGLAGGALAAWSGRLPRFVLEGLWMAAVGI
                     SLAIADVSLQTGFIALFGLQGIVMLLVGTAVFVHFLRQPVGPGE"
     gene            823..1119
                     /locus_tag="VFQ79_13465"
     CDS             823..1119
                     /locus_tag="VFQ79_13465"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU0140724.1"
                     /translation="MSARIQKLKDVDRIIHEPARLMVMALLNSVQEADFQYLHYSTGL
                     TKGNLSVHLSKLEEAGYISIEKTFRGKYPLTICRLTEQGRKVLDDYRKIMRGAL"
     gene            complement(1155..1400)
                     /locus_tag="VFQ79_13470"
     CDS             complement(1155..1400)
                     /locus_tag="VFQ79_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140725.1"
                     /translation="MPKIEKWDSLPPGVRQHLMDRLRDRSIQIGDLNKLRLWIESNPE
                     VPEGDWYKDFGSFKICGHGSYPKTFLCRGQVAKGEAV"
     gene            complement(1400..1585)
                     /locus_tag="VFQ79_13475"
     CDS             complement(1400..1585)
                     /locus_tag="VFQ79_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140726.1"
                     /translation="MAKTRRLSANRMLVDLIENGLEAERRKQQEFTELAERFRSATDP
                     EEVKRLGDQLGRMIFGD"
     gene            complement(1757..3025)
                     /locus_tag="VFQ79_13480"
     CDS             complement(1757..3025)
                     /locus_tag="VFQ79_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0140727.1"
                     /translation="MTSKERVVAAICHREPDRIPVDLGGTAVTGIHVSCVAALREHYG
                     LEKRPVKVHEPYQMLGWVDDDLKQAMGIDVEGVFARKTMFGFPNEDWKSWRTQDGLEV
                     LVPGGFNVTVDANGDTLMYPEGDTTAPPSGRMPKSGYFFDTIVRQEPIDEDNLDVEDN
                     LEEFGPVEEIDLAYFRTTAREASATGRAVVANFGGTAFGDIALVPAPWLKHPKGIRDI
                     AEWYMSTTSRRDYIHRIFSYQCDVAVQNLEKIHAVIGDSVDVVFLCGTDFGTQTSSFC
                     SVAAFRDLYFPYYKRVNDWIHAHTGWKTFKHSCGSVERFIPSFIEAGFDILNPVQCSA
                     TGMGAEHLKSTYGDRLVFWGGGVDTQKVLPFGTPEEVRRQVIERCTVFSRGGGFVFNT
                     IHNIQAQTPVANIVAMLDAVREFDRAGASV"
     gene            complement(3060..4076)
                     /locus_tag="VFQ79_13485"
     CDS             complement(3060..4076)
                     /locus_tag="VFQ79_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0140728.1"
                     /translation="MPIGADLVLHELPDPEAAARDGARLGRVLEEAARRYNTPLAFPL
                     MDLRLEKADLLQHFAIAETEVDAFHFREAPGEHAAEEARIAGNAGFPGRNRAHIHSVR
                     YIAESTELVPVGMAIGPFSLLTKLMADPIPALAMAGMGVGAEEDPDVLMAERCLALAE
                     AAVQRSIAAQIDAGAKLILICEPAASIVYISPRQMEAGSGLFDRFVIEPHLRIKEQLE
                     AAGMGLIFHNCGELTDGMVRSFAERLRPMILSLGSSRQLWLDADLVSDDIVLFGNLPT
                     KKFYSDAVISDEEVARLTCELAERMRDTRQPFILGSECDVLHVPDAAETIRRKVRVML
                     TAGQ"
     gene            4351..5607
                     /locus_tag="VFQ79_13490"
     CDS             4351..5607
                     /locus_tag="VFQ79_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140729.1"
                     /translation="MASDTNSSPGFKRRSFLGALGSTAAAGAANAQETPAAKPAPKRI
                     RVGLIGAGNRGSYLARASDQLGKDGEDIEIVAICDIYQPRRERVATRFRAKEYKRSID
                     LVNDPNVDVVLVATPDRLHATHALEAIRAGKDVYSEKPATHWQQFDQLKELVRAAREH
                     KTVYQMGTQRLANPVWRQAADSIKRGSIGKPVHVQMGYFRRGDSGERGMPIDDPNAKP
                     GPDLDWEAFQGDAPRHSFSIERFFQWRMFMDYSGGPCTDNNVHFLSLMIKALGLDFPS
                     RVVALGGKYVFNGDREVPDTFDMVAEYPSGLNFVFMGTYANDVPVDTVVRGTEGTLTV
                     KDEPDGMVQPLAGITRRPQGLGYGIDVNSEHLRDFYRSVRTREKPQGDIELAYRTQVL
                     LMMAMRSFLERKVAAFDAAAERIVMA"
     gene            complement(5617..6795)
                     /gene="moeB"
                     /locus_tag="VFQ79_13495"
     CDS             complement(5617..6795)
                     /gene="moeB"
                     /locus_tag="VFQ79_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEU0140730.1"
                     /translation="MAVETAAEQAALSNEEILRYSRHLIMPEVGMDGQLKLKQAKVLL
                     IGAGGLGAPLGMYLAAAGVGRIGMVDFDVVDFTNLQRQVIHGTRDVGKKKLDSAAETM
                     ADINPHIRIDKYETALSSENALELFKEYDMVIDGTDNFPTRYLVNDACVLTGKPNVYG
                     SIFRFEGQATVFGYPGGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGTIGLIQATEAV
                     KLILGVGELLVGRLLLYDALAMRFRELKLRRDPACPVCGDNPSIHELIDYHQFCGVPQ
                     QQGAAAETAPGAADIDPTEVKAKIDRSESFVLIDVREPHEYQICKIPYARLIPLGELP
                     KRVNELNSADEIVAHCKSGVRSAKAVDFLKQAGFKKVRNMRGGILAWSDKVDPSVPKY
                     "
     gene            complement(6785..7069)
                     /locus_tag="VFQ79_13500"
     CDS             complement(6785..7069)
                     /locus_tag="VFQ79_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU0140731.1"
                     /translation="MAKILIPTPLRQYVEKKDSIEIGGATVGEALGTLTSEYQDLRRH
                     LYNDEGKLRSFVNVYVNDEDIRYLSKESTPLKEGDTVSIVPSIAGGLNGS"
     gene            complement(7069..7494)
                     /locus_tag="VFQ79_13505"
     CDS             complement(7069..7494)
                     /locus_tag="VFQ79_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEU0140732.1"
                     /translation="MIRIEKNAWKVMLDHAKSIYPNECCGAMLGNVDGGDKLVRTAVP
                     LDNAFSGPQAQRYELRPEDLLRADREARAKGMDLVGIFHSHPDCDAYFSETDLKNSCP
                     WYSFVVLSMRKGEFDHANSWLPNPEQTAADKEHLEIVED"
     gene            complement(7481..8425)
                     /locus_tag="VFQ79_13510"
     CDS             complement(7481..8425)
                     /locus_tag="VFQ79_13510"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEU0140733.1"
                     /translation="MLPALSKSSRSQSLLAQIGNTPLIHLERIASDLPGVEILGKAEF
                     FNPGGSVKDRPALNMILDGERTGRLLPERILLDATSGNTGIAYAMIGAVRGYRVRLCL
                     PANASEERKRMLKAYGAEMILTDAGEGSDGAIRKVRELYEADPDLYFYPDQYSNPANW
                     KAHFETTAVEIMEQTGGGITHFVAAMGTSGTFVGTSRRLKRDLPGVRCISAQPSSGFH
                     GLEGLKHMPTAIVPSIYDESLADDNIWIETEDAYRMVRRLAREEGLLVGISSGANLVA
                     AREIGRRLAAAGEPGVIVTVLCDGAEKYLSEPFWNDSD"
     gene            complement(8568..9239)
                     /locus_tag="VFQ79_13515"
     CDS             complement(8568..9239)
                     /locus_tag="VFQ79_13515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0140734.1"
                     /translation="MSLTNTPVDARLWAYIQDVSRREPDLLRRVREETARLPEAHMQI
                     TPEQGQFLGLLVRLLEATQVLEVGVFTGYSSLSLALALPDHGRIVACDRSEEFTSMAR
                     RYWREAGVEHKIDLRLGPALDTLDGLLSERRKGTFDLAFIDADKQNYDNYYERALLLI
                     RPGGLIAIDNVLWHGVVIDETTNDDSTIAIRTFNRKIHQDDRVWVSMVPIGDGLTLVL
                     KKQQP"
     gene            complement(9240..>9858)
                     /locus_tag="VFQ79_13520"
     CDS             complement(9240..>9858)
                     /locus_tag="VFQ79_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU0140735.1"
                     /translation="RRVTRLEADGSLTVLASHFEGKRLNSPNDLVYKSDGALYFSDPP
                     YGFPKQDDDPEKELDFCGLYRLAGGELQLLYKDLRRPNGLAFSPDEKYLYVSNSDPQR
                     RIWMRFEVQGDGSIANGEVFYDVTNEAAAGSPDGLKVDKQGNLYCTGPLGVWVFTPGG
                     KLLGRIEPPERPANCHWGGADAKTLYITAQTSLYRIRTNIEGIRP"
BASE COUNT         1963 a         3013 c         2810 g         2072 t
ORIGIN      
        1 aatggcctgc cggacgtcct ggatgacgtt ggcagatgcc acgcaagtca ggaccaaggc
       61 gatcccagag attctgagga gcttcattgg ttccaggata tcagcaaggg tagagcggaa
      121 acgtacgcgg ctcttgacaa caacctgccg gggattcata attctataga ctagtctaca
      181 atacagacta ctctacggaa ggaattatgg aaaactcgag tccgcacacg atcgcgcggc
      241 aactcatcca gaaggctcat aacttcgacg gattgccgga ggtggtggtc ggcttcttct
      301 tcctgctggt ttccgccttg ctgtatgtct taaccgtact gcccaaggga acacccggct
      361 tccaagcggc cgttctcatt cttgctttcg gcgtgcccat attctgcttt agcgccaggc
      421 cgttgctcaa ccgggtgcgg cgccggtatc tggtcgggag agagggatat gtacgaccca
      481 aacgggcggg ccggaaagag atagccaccg gaattgtgtt ggctgttctt gtggccgccg
      541 tcctgtttgt cctggttcct cgcctgtcgc acccggaccg ctggctgctg gccggaaccg
      601 gacttgcagg aggcgcgctt gccgcctggt ccggccggct gccccggttc gttctggaag
      661 gactctggat ggcagcggtt ggcatctctc tggcgattgc ggacgtttcc cttcaaaccg
      721 gattcattgc cctgttcggc ctccagggta tcgtgatgct tctcgtgggc actgctgtat
      781 ttgtgcattt tctccggcag ccggtcgggc cgggcgaata agatgagtgc gagaattcag
      841 aagctgaagg atgtggatcg catcatccac gaaccggcac ggctgatggt gatggccctg
      901 ctgaactccg tccaggaggc cgacttccaa tacctgcatt attccaccgg gctcaccaag
      961 gggaacttat ccgttcattt gtccaagctg gaagaagcag gctatatctc catcgagaaa
     1021 acgttccggg gaaaatatcc gcttactatt tgccgcctga cggagcaggg ccggaaagta
     1081 ctcgacgact atcgcaagat catgcggggg gcgctgtagg ggaggtggcc ggatcaaacc
     1141 gcttggtggc ccaatcagac ggcctcgccc ttcgctactt gtccgcgaca caaaaatgtt
     1201 ttgggatatg acccgtgacc gcagattttg aaggaaccga agtccttgta ccaatcgcct
     1261 tctggcacct ccgggttcga ttcgatccag agccggagtt tattcaggtc gcctatctgg
     1321 atcgatctgt ctcgcaatcg gtccatcaga tgctggcgga caccgggcgg caagctatcc
     1381 cacttttcga tcttcggcat cagtcgccga agatcatacg gccgagttgg tcgcccaggc
     1441 gcttaacctc ttccgggtcg gttgcactgc ggaaacgctc tgcgagttcg gtgaattcct
     1501 gctgcttgcg cctttcggcc tcaagcccgt tctcaatgag atccaccaac atccggttag
     1561 cactaaggcg ccgggtcttc gccatagtgc gaacctgtgc ggcgacatta gcggggaggc
     1621 taacgctttg ccgaatcgtt ttgcttctca ctacaccatt atgcaccaaa atggtgcata
     1681 aatgagcacg gcaaacgcgg gtcacctccg gacacgcctt ttgtccatgc tcaggcgact
     1741 catcgccgct ccgctctcac acactcgccc cggcgcggtc gaattcccgc accgcgtcca
     1801 gcatcgcaac aatattcgct accggtgttt gagcctgtat gttgtgaatc gtgttgaaga
     1861 caaatccgcc gccccgggaa aacactgtgc atcgctcgat cacctggcgg cggacctcct
     1921 ctggcgtccc gaacgggagc accttttgcg tatccactcc tccgccccag aaaacaaggc
     1981 gatcgccgta ggtggacttc aaatgctccg cccccatgcc cgttgccgag cattgcaccg
     2041 ggttcagaat gtcgaagccc gcttcgataa acgatggaat gaaccgctcc accgacccgc
     2101 aagaatgttt gaatgtcttc cagccagtat gggcgtgaat ccaatcgttg acgcgcttgt
     2161 aataggggaa gtagagatcg cggaacgccg ctacggaaca gaaactggag gtctgggttc
     2221 cgaagtcggt cccgcaaaga aagacgacat ccacagaatc gccaatgaca gcgtggatct
     2281 tctccaggtt ctgaacagcc acgtcgcact ggtaggagaa gatgcggtgg atgtaatcgc
     2341 gccgcgaggt cgtggacatg taccactccg cgatgtcgcg gattcccttc ggatgtttga
     2401 gccacggcgc gggcaccagg gcgatgtcgc caaacgcggt gccgccgaag ttcgcaacca
     2461 cggctctccc ggtcgccgag gcttccctcg ctgttgtccg gaagtaggcc agatcgatct
     2521 cctcgaccgg tccgaactcc tccaggttgt cctccacgtc caggttgtct tcgtcgatgg
     2581 gctcctgccg gacgatggtg tcaaagaagt atccactttt cggcatgcgt ccgctcggcg
     2641 gcgccgtggt gtcgccctcg gggtacatca acgtgtctcc gttcgcatca acggttacat
     2701 tgaagcctcc cggcaccagg acctcaagcc cgtcctgcgt ccgccagctt ttccagtctt
     2761 cgttcgggaa gccgaacatg gtcttgcggg cgaacacccc ctccacatcg atccccatcg
     2821 cctgcttcag gtcgtcgtcc acccagccga gcatctggta aggctcgtga actttgacag
     2881 ggcgcttttc aagcccatag tgctcgcgca gcgccgccac gcaactgacg tgaattccgg
     2941 ttacggcggt cccaccgaga tcgaccggta tgcggtccgg ttcccggtgg cagatggcgg
     3001 ccacgaccct ttctttactc gtcattccgg aattcagtgt accagacccc aatgcccggt
     3061 tattggcctg ctgtcagcat cacccgcact ttgcgccgga tagtttccgc cgcgtccggc
     3121 acatgcagga catcgcactc ggacccgagg atgaacggct gtctggtgtc gcgcatccgc
     3181 tccgccagtt cgcacgtcag gcgcgccact tcctcgtcgc tgatcacggc atccgagtaa
     3241 aacttcttcg tcgggaggtt gccgaagagt acgatgtcgt ccgagaccag gtccgcatcc
     3301 aaccagagct gccgtgaact gccgaggctg agaatcattg gccggagccg ctcggcgaaa
     3361 gaacgaacca tgccgtccgt cagctctccg caattgtgga atatcaatcc catgccggcg
     3421 gcttcaagct gttctttgat tcgtaagtgc ggctcgatca caaagcggtc gaacagtccg
     3481 gagccggcct ccatctgccg cggagagata tacacgatgc tggcggcagg ttcgcaaatc
     3541 aggatgagct tggcgccggc gtcaatctgc gcggcgatcg accgctggac ggcggcctcg
     3601 gccagcgcca ggcaacgctc ggccatcagg acatccggat cttcttcggc gcccaccccc
     3661 atcccggcca ttgcgagtgc gggaatcggg tccgccatca gcttagtcaa cagcgagaac
     3721 ggcccgatgg ccatgccgac cggaaccagt tccgtcgatt cggcgatata ccttacagag
     3781 tggatatgcg cgcgattgcg gccggggaaa ccggcgtttc cggctatgcg ggcctcttcg
     3841 gcggcatgct cacccggcgc ctcccgaaag tgaaaggcat ccacttcggt ttcggcaatc
     3901 gcaaaatgct gtagcagatc ggccttttcc aggcgcaggt ccatcagagg aaaggccagc
     3961 ggtgtgttgt atcgccgcgc ggcctcttcg aggacacgcc cgagccgcgc tccatctcgc
     4021 gccgccgctt ccggatccgg cagttcgtgc aaaacgagat ccgccccgat cggcattcgc
     4081 aaacctcgcc ggcccaactc gatacaatac tcgcgattga tcctcacgaa gcgtagtgtc
     4141 gcacatgata agctgcacta aacacgatgt cctaaatgtt tgcgcaacga tttgattagc
     4201 cacggatgga cacagatgaa cacagataag aaaaacgcaa ggcttgatcc gtgtgaatcc
     4261 gtgttcatcc gtggccgaag ttgcttttct agattgctga gatgcgcaat tatttatgac
     4321 gttctttaga tcaggaggag catcccggat atggccagcg atacgaattc atcacccggt
     4381 ttcaagcgcc ggagctttct tggcgcgctc ggctcaacag ccgctgccgg agcggcaaat
     4441 gcgcaagaga ctcccgcagc caagcctgca cccaagcgca tccgcgttgg cctgatcgga
     4501 gcgggaaacc gcggttccta tctcgcccgc gccagcgacc agcttggcaa agacggcgag
     4561 gacatcgaga tcgtagcaat ctgcgatatt taccagccgc gccgcgaacg ggtggctacg
     4621 cgtttccgcg cgaaagaata caagcgttcc atcgatctgg taaacgaccc gaatgtcgat
     4681 gtagtgctgg tggctacgcc ggaccgtctg catgcgacgc acgcgctgga ggccatccgc
     4741 gccgggaagg acgtttacag cgaaaagccg gcgacgcact ggcagcagtt cgatcagctc
     4801 aaagaactgg tgcgcgcggc gcgcgagcat aaaaccgttt accagatggg cacacagcgg
     4861 ctcgcgaatc cggtctggag gcaggccgcc gattcgatca aacgcggttc catcggcaag
     4921 cccgtgcacg tgcaaatggg ctacttccgc cgcggcgatt cgggagagcg cggaatgcct
     4981 atcgacgatc cgaacgccaa gcccggtccg gatctcgatt gggaagcgtt tcaaggcgat
     5041 gccccgcgcc actcgttttc catcgagcgt ttcttccagt ggcggatgtt catggattat
     5101 tcaggtggcc cgtgcacgga caataacgtg catttcctgt cgctgatgat caaggccctg
     5161 gggttggact ttccatcgcg ggtagtcgcg ctgggcggca agtatgtatt caatggagac
     5221 cgcgaagtgc ccgacacttt cgacatggtg gccgagtatc cgagtgggct caatttcgtc
     5281 ttcatgggca cctatgccaa cgacgttccc gttgataccg tggtgcgcgg taccgaagga
     5341 acgctgaccg tcaaagacga gccggacggc atggtgcagc cgctggccgg catcacccgc
     5401 aggccacagg gtctgggtta cgggatcgac gtcaattcgg agcaccttcg cgacttctat
     5461 cgttccgttc gtacacggga gaagccacaa ggcgacatcg agctcgccta tcggacgcag
     5521 gtgctgttga tgatggcgat gcggtcgttc ctggagcgca aagtggccgc ctttgacgct
     5581 gcggccgaac gcatcgtaat ggcctgagcc gcgccgtcag tattttggga cgctcgggtc
     5641 caccttgtcg ctccaggcga ggatgccgcc gcgcatgttg cggaccttct tgaatcccgc
     5701 ctgcttgagg aaatcgacag ccttggcgct tcgcacgccg ctcttgcagt gggccacgat
     5761 ttcgtcggcg gaattgagct cgttcacacg cttgggcaac tctccgagcg ggatcaaacg
     5821 cgcgtaaggg atcttgcaga tctggtactc gtgcggttcc cggacatcga tgagcacgaa
     5881 ggactcgctc cggtcgattt tcgctttcac ctcggtggga tcgatatcgg cagctcccgg
     5941 agcggtttcc gcggccgcgc cctgctgctg cgggaccccg cagaactggt gataatcgat
     6001 cagctcatgt atcgacggat tgtcgccgca aaccgggcat gccgggtcgc gccgcaattt
     6061 caactcgcgg aaacgcatcg ccagggcgtc ataaagcagc agcctgccca ccagcaactc
     6121 gcccacgccc aggatcagct tcacagcttc ggtcgcctgg atcaagccga tggtaccggg
     6181 aagaatcccc agcaccccgc cttcggcgca gcttggcacc agtcccggcg gcggcggctc
     6241 cggataaagg cagcggtaac acggcccgcc cggatacccg aacacggtgg cctgcccttc
     6301 gaaacgaaag atggaaccgt agacattggg cttgccggtg agcacgcagg catcgttcac
     6361 caggtaacgc gtcgggaagt tatcggttcc gtcgattacc atgtcgtact ctttgaacaa
     6421 ttccagcgca ttctcgcttg aaagcgccgt ctcgtacttg tcgatgcgaa tgtgcgggtt
     6481 gatatccgcc atggtttcag cggcggaatc gagctttttc ttgccgacgt cacgggtgcc
     6541 gtgaatcacc tggcgttgca ggtttgtaaa gtcgacgacg tcgaagtcca ccatgccgat
     6601 gcgcccgacg cctgccgccg ccagatacat tccgagcggg gcgcccagtc cacccgcgcc
     6661 gatcagcaat actttcgcct gcttgagctt caactgcccg tccatgccca cttcgggcat
     6721 gatcaggtgg cggctgtagc gcaggatctc ctcgttggag agcgccgcct gttcggctgc
     6781 cgtctcaact gccattcaaa cctccggcga tgctgggcac gatggaaacc gtgtctcctt
     6841 ccttgagcgg agtggattct ttgctcaggt agcggatgtc ctcatcgttc acatacacgt
     6901 tcacgaaact ccgcagcttg ccttcatcgt tgtaaaggtg ccgccgcaaa tcctggtact
     6961 cgctggtgag cgttccaagc gcttcgccga cggtcgcgcc gccaatctcg atggagtcct
     7021 ttttctcaac gtactgccgc agcggggtcg gtatcagtat cttcgccatc agtcctcgac
     7081 aatctccaaa tgttctttat cggccgccgt ctgttccggg ttgggcagcc agctattcgc
     7141 gtgatcgaac tccccttttc gcatggaaag caccacgaag gaataccacg gacaggagtt
     7201 ctttaaatcc gtttccgaga agtaggcgtc gcaatcggga tgggaatgaa agatcccgac
     7261 gagatccatc cccttggccc gtgcctcccg gtcggcccgg agcaggtcct ccgggcgcaa
     7321 ttcataacgc tgagcctgcg gaccgctgaa ggcattatcg agcggcacag cagtcctgac
     7381 gagtttgtcg ccgccatcca cgttgcccag cattgcgccg cagcactcgt ttgggtaaat
     7441 cgatttggcg tggtccagca tcactttcca cgcattcttt tcaatccgaa tcattccaga
     7501 acggctcact cagatatttt tcggccccgt cgcaaagtac cgtgacaatg acgccgggct
     7561 ctcccgcggc ggccagccgc cggccgattt cccgggcggc cacaagattt gcgcccgaag
     7621 aaatgcctac cagcagccct tcctcgcggg cgaggcgccg caccatccgg tacgcatcct
     7681 ccgtctcgat ccagatgttg tcatcggcca gactctcgtc gtagatggag ggcacaatcg
     7741 ccgtcggcat atgcttcaga ccttccaggc cgtgaaatcc ggacgacggc tgcgcggaga
     7801 tacaccggac gcctggaaga tcgcgtttca gccgccgcga ggtaccgacg aaagttccgc
     7861 tcgtccccat ggcggccacg aagtgggtga tgccgccgcc ggtctgctcc atgatttcga
     7921 ctgcggtggt ttcgaaatgc gccttccaat tggccggatt gctatattga tcggggtaga
     7981 agtaaaggtc cggatcggct tcgtacaact cgcgcacttt gcgaatcgcg ccatccgatc
     8041 cttcgccggc atcggtcagg atcatctcgg cgccataggc ttttaacatg cgcttgcgct
     8101 cttccgatgc attggccggc aggcacaacc ggacacgata accccgaacc gctccgatca
     8161 tggcataggc gattccggtg ttgccgctcg tcgcatccag caggatgcgc tccggcagaa
     8221 ggcggccggt gcgctctcca tccagaatca tattgagagc cggccggtcc tttacagaac
     8281 ctcccgggtt gaaaaactcg gctttcccca ggatctcgac gccgggcaga tcgctcgcga
     8341 tccgctccag gtgaatcagc ggtgtgtttc cgatttgcgc cagaagcgat tggctgcgag
     8401 acgatttgga taaggccggc aacatgctac agggagacta aacccagtat gtactacatt
     8461 ctcatcaatc tgcccaatct ctgtcaagaa taggacgatt gtgagaatta cctctaaata
     8521 ttagagaact ttgctactcc cggagtcgca gaaaatccgg gttccattca gggttgctgt
     8581 ttcttcaaca cgagggtcag cccgtcgccg atgggaacca tgctgaccca cacccgatcg
     8641 tcctggtgaa ttttgcgatt aaacgtccgg atcgctatgg tggaatcgtc gttcgtggtt
     8701 tcatcgatca ccacaccatg ccagagtacg ttgtcgatgg caatgagtcc gccaggccgg
     8761 atcaggagca gggcccgctc gtaatagttg tcgtagtttt gcttgtcggc atcgataaat
     8821 gccaaatcga aggtgccctt gcgccgctct gaaagcaggc catccagcgt gtccagcgcg
     8881 ggacctaagc gaagatcgat cttgtgctcg acgccggctt cccgccaata ccgccgcgcc
     8941 atggaggtaa actcttcgct tctatcgcac gccacaatcc ggccgtggtc cggtagcgca
     9001 agggccaggg aaagagagct gtacccggtg aagacaccca cttccaggac ctgggttgct
     9061 tccagaaggc gcacaagcag tccgaggaac tgcccctgct ccggagtgat ttgcatgtgc
     9121 gcttccggca gcctggcggt ctcttcccgc actcgccgca aaaggtcagg ctcgcgccgc
     9181 gaaacgtcct gaatatacgc ccagagccgc gcatctaccg gagtgttcgt gagcgacatt
     9241 tacggccgga tcccttcgat attcgtccgg atgcggtaaa ggctggtttg cgcggtgatg
     9301 tacagggttt tcgcatccgc gccgccccag tggcaattcg cgggtctctc cggcggctcg
     9361 attctgccta acagttttcc gcccggcgtg aagacccaca cgccaagcgg cccggtgcaa
     9421 tagaggttgc cctgtttgtc gactttgagt ccatcgggtg agccggcagc cgcctcgttc
     9481 gttacgtcgt agaacacttc gccatttgca atcgaaccat cgccctggac ttcaaaacgc
     9541 atccagatcc ttcgctgggg gtccgagttg gagacataca aatacttctc gtccggcgaa
     9601 aaggcaagac cgttcggacg cctcaggtcc ttgtacagaa gttgcagttc cccgccggcc
     9661 aggcgataaa ggccgcaaaa atccaattct ttttcgggat cgtcatcctg cttcggaaag
     9721 ccgtagggag gatcactgaa gtaaagggcg ccgtccgact tatacaccag atcgttcgga
     9781 ctgttcagtc gctttccctc aaagtgagaa gccagaaccg tgaggctgcc atccgcttcc
     9841 aggcgcgtca cacgccgg
//
LOCUS       DASTMK010000308         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_16683, whole genome shotgun sequence.
ACCESSION   DASTMK010000308 DASTMK010000000
VERSION     DASTMK010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16683"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1474
                     /locus_tag="VFQ79_13525"
     CDS             104..1474
                     /locus_tag="VFQ79_13525"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU0140736.1"
                     /translation="MSQATDAFVWDNQERFLDELKQFLKIPSISTLPEHRADIDRAAE
                     FCAANLRKAGLENVEIVPTGRHPLVYADWLHAPGKPTVLCYGHYDVQPPDPLEEWKTP
                     PFEPSVRDGNLYARGSADDKGQMLMHIKAIETLFAVNGTLPVNVKFLIEGEEEVGGAS
                     IAKYVSANQDKLKADVGLVSDTALYAEGIPTLCIGLRGLVYFEVAATGAGRDLHSGLY
                     GGCAPNPVFGLIELLAKAKNEKGVIQVPGIYEDVESPAAAELESWKKLPFDEIDFLER
                     EVGACRLTGETDFSVLERIWARPTLEVHGIAGGFTGTGAKTVIPAKATAKVSFRLVPK
                     QDPEKVIASFREWVRKNTPDGLQTDVAVLSASPAVVVNPDHPAIRTAAGAFSDILQKE
                     TVFIRSGGSIPIVGDFATHLGIPTVLMGFGLPDDGLHSPNEKFKIQNYYNGIMTVAHF
                     LEQYGA"
     gene            1506..>3005
                     /gene="murJ"
                     /locus_tag="VFQ79_13530"
     CDS             1506..>3005
                     /gene="murJ"
                     /locus_tag="VFQ79_13530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU0140737.1"
                     /translation="MDTTTDETSPSERVARSAGVVSAAVALSRISGLVREVLMAGLFG
                     AGMAYDAFLLGFRVPNLARDLFAEGALSSAFVPTFTAYLARSRREAAELANQVATAVF
                     LVIGVVCLAGILLSPAIVSVMAPGFSEVQGKSELAVHLMRIMFPFLLLVALAAQAMGI
                     LNSCNQFGVPALSSTFFNLGSVAFGLALGFWWGPSLGISQLEGMAYGVVIGGALQLAW
                     QLPALWRAGFAFKLRFDWSHPGLRHIVRLMGPAILGGAAVQVNIVVNTFFASQIVDPV
                     RGMDGPTSWLNYAFRFMQLPLGLFGVSIAAATLPSISRSITSGDMEEYRTTLARSLGM
                     VFLLTIPSSVGLMVLGETMIGAVYQSGRFEVYDTRQTALALSYYAIGLSGYAALKVLA
                     PAFYALGDARTPMMVSVLSIGMNLGLAATLVHVWGMGHAGLALSTSLVAVFGFAVLFA
                     VLRRRIGGIHGWHLLSGFSRTLVAAAVMGVATAGIHMGLVSLLGISKLGR"
BASE COUNT          537 a          845 c          962 g          661 t
ORIGIN      
        1 aaaagcacag tgaaagcggc cgccgcctgc gcggcgagca gttagttgcg caaccggagc
       61 ctttttcaga acaatcccct gccgttccat ctacaatgtg gatatgtcgc aggcgaccga
      121 cgcattcgtg tgggataacc aggaacgctt cctggacgaa ctgaaacaat ttctgaagat
      181 tccgagcatc agtactcttc cggaacaccg cgccgatatc gaccgggcgg cggagttctg
      241 cgccgcaaat ctgcggaaag cgggactcga aaacgttgag atcgttccaa caggcaggca
      301 cccgctggtg tatgccgact ggctgcacgc tcccggcaag ccgacggtgc tttgctatgg
      361 gcactacgat gtacagcctc ccgacccgct cgaagaatgg aagactcctc catttgagcc
      421 gtcggtccgt gacggcaacc tttatgcgcg cggttcggcg gacgacaagg gccagatgct
      481 gatgcacatc aaggccatcg aaacgttgtt tgcggtgaat ggcacgctgc cggtaaacgt
      541 gaagttcctg atcgaaggcg aagaagaagt cggtggggca tccatcgcca agtacgtatc
      601 cgcgaaccag gacaagttga aggcggatgt cgggctggtc tccgataccg ctctctatgc
      661 ggaaggaatc cccacgctct gtatcggact gcgcgggctg gtttactttg aagttgcggc
      721 gacgggagcc gggcgcgacc tgcattccgg gctgtacggt ggatgcgcgc ccaacccggt
      781 gtttggcctg atcgaactgc tggcgaaagc caaaaacgaa aagggtgtta tacaagtgcc
      841 gggcatctac gaagacgtgg agtctccggc ggcggcggag ctcgagagtt ggaaaaagct
      901 gcctttcgac gagattgatt ttctcgaacg ggaggtgggt gcctgccggc tgaccggcga
      961 gacggatttt tccgtgttgg agcgcatctg ggcccgtccg acgctggaag ttcatggaat
     1021 tgccggcggc tttacaggga ccggtgcgaa gaccgttatt ccggctaagg caactgcgaa
     1081 agtcagcttc cggctggtgc cgaaacagga ccctgagaag gtgatcgcgt ccttccgcga
     1141 atgggtccgc aagaacacgc ccgacggtct tcagaccgac gtggcggtgt tgagcgcctc
     1201 gcccgccgta gtggtgaatc ccgaccatcc cgcgatccga accgcggcag gggcattctc
     1261 cgatattctt caaaaagaaa cggtcttcat ccgatccggc ggatcgattc cgatcgtggg
     1321 cgatttcgca acccacctgg gcatccccac ggtattgatg ggcttcggtc tgccggatga
     1381 cggtttgcat tcacccaatg agaagttcaa aatccagaac tactataacg gcatcatgac
     1441 agtagcgcac tttctggaac agtacggcgc ctgaacgttg cccgcatttc gcctgagggg
     1501 tttgactgga cacgactacc gatgagacat ccccatccga acgcgtagcc cgttcggcgg
     1561 gagttgtctc ggccgctgtg gctttgagcc ggatcagcgg actggtgcgc gaagtcctga
     1621 tggcgggcct gttcggcgcc ggcatggcgt acgatgcatt tctgctgggc ttccgggttc
     1681 cgaacctggc gcgggacctg ttcgccgagg gagcactctc gtccgctttt gttccgacat
     1741 tcacggccta cctggccagg agccgcagag aagcggctga actcgcgaac caggtggcga
     1801 cggccgtgtt cctggttata ggcgtggtct gcctggccgg gatccttctc agcccggcaa
     1861 tcgtcagtgt catggcgccg ggcttctcgg aggtgcaagg aaagtccgaa ctggctgttc
     1921 acctgatgag gatcatgttt cctttcctgc tgctggtggc tctggccgcg caggcgatgg
     1981 gaattctgaa ttcctgcaat cagtttggtg ttccggcgct gtcctccacg tttttcaacc
     2041 tcggttcggt ggcattcggg ctggcactcg gtttttggtg gggaccgtcg cttggcattt
     2101 ctcagcttga agggatggcc tacggtgtcg tcatcggtgg cgcgttgcaa ctggcctggc
     2161 aactcccggc gttatggcgc gccggcttcg cattcaagct gcgtttcgat tggtcgcacc
     2221 cgggattgcg ccacatcgta cggctcatgg gtcccgcgat tttgggcgga gccgcggtgc
     2281 aggtgaacat cgtggtgaac acgtttttcg cctcgcagat cgtggatccc gtccgcggca
     2341 tggacgggcc caccagttgg ttgaactatg cgttccgttt catgcaactg ccgctcgggt
     2401 tgtttggggt gtcgattgcg gcggcgaccc tgccgtcgat ttcgcggagt atcacatcag
     2461 gcgacatgga ggagtaccgg acaacgctcg cccgctcact gggcatggtt tttctgctga
     2521 ccattccctc gtcggtaggc ctgatggtgc tgggcgagac gatgattggc gccgtgtatc
     2581 agtcgggccg gttcgaagta tatgatacgc gccagaccgc gctcgcgctg tcgtattatg
     2641 cgatcggcct tagcgggtat gccgccttga aagtactggc gcccgccttc tacgctctcg
     2701 gcgacgcgcg aacgcccatg atggtgagcg tgctttccat cggcatgaat ctgggactgg
     2761 cggcgacgct ggttcacgtt tggggcatgg ggcatgcagg tctggctctt tccacatcgc
     2821 tagtggcggt gtttggtttc gcggttctgt tcgcggttct ccgccggcgc atcggcggca
     2881 tacacggttg gcatctgctc agcggcttct cgcgtaccct ggttgccgct gcggtgatgg
     2941 gggtggcgac agccggaata cacatgggcc tggtgtcgtt gctgggaatc tccaaactgg
     3001 gccgg
//
LOCUS       DASTMK010000309         4698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_111210, whole genome shotgun sequence.
ACCESSION   DASTMK010000309 DASTMK010000000
VERSION     DASTMK010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111210"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFQ79_13535"
     CDS             <1..348
                     /locus_tag="VFQ79_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEU0140738.1"
                     /translation="YWRLEPYIGFGADAHSFDGKTRRRNVEGAGEYVDRIRRGLDPCA
                     AETPAELSEERFFVGLRLEEGIRPASSELARFAEPIRRFLDDGLLAMEDGLLRLTNRG
                     VLLSNEVFQEFLT"
     gene            345..1403
                     /locus_tag="VFQ79_13540"
     CDS             345..1403
                     /locus_tag="VFQ79_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEU0140739.1"
                     /translation="MSEQPLVDLRSDTVTRPTASMRRAMAEAEVGDDVYREDPTVNRL
                     EERAAAMFAKQAALFVPSGTMGNTIAVKLHTEPGQEVICEAKAHIVTYELSMLAWFSG
                     CLARTIPTAHGILTWEQIRREIRPPAPFWAPTGLIEIENTHNMAGGTIYPIEAVDEIC
                     HEAHALGLKVHMDGARIFNAAAALGISVARIAVHVDTVMFCLSKGLAAPVGSMLVGSR
                     DDMDRARLHRKRLGGGMRQAGVLAAAALVAIEEMTERLVDDHANAAFLAEELANIRGI
                     SLDPRRVETNIIIFDVSGTGITAADFSACLKSRGILMNPVSPTEIRAVTHCDVTRRDC
                     ERALQTITEIARSAAASA"
     gene            complement(1407..2141)
                     /locus_tag="VFQ79_13545"
     CDS             complement(1407..2141)
                     /locus_tag="VFQ79_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140740.1"
                     /translation="MLLLLLTLVATLAAAAGIRLTSMPAISRKLVPFSGGLLIGICAF
                     GIVPEVVEELEWYASLLWLLAGFLALWVIDRFVYPVCPSCSHSHDHDVCITRLHGFAT
                     PMIVAASAHSFLDGFGAAASYTAGPGTMLLFAIAMHKIPEGLAFGIILRSAVQSPWKA
                     LAWCAAAESAMFVGGGFESLLTGQMTAAWFGVLLAVIGGSFSYLGLHAIHGEFKRRGL
                     VPAFMPALTGAAGAVALQQGLRVFLR"
     gene            2338..4524
                     /locus_tag="VFQ79_13550"
     CDS             2338..4524
                     /locus_tag="VFQ79_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU0140741.1"
                     /translation="MQQYGEQQTANLSSQGDLYRELFENATDIIYIHDLDGRFTAVNK
                     AIERITGYCRAEILQMSLADLAVPEYQELARQITFEALGGGTSEPVEFVIRSKAGKRI
                     VLEVTSRLLFQDGRPVGVQGFARDISARRQLEQDRNRVLEMVAKNIELPEVLNELMGM
                     VQRQRPGIAGAVLQRRGDRLYVIAAPDLPPAYFDHMRGTYADRRGGCCGAAVEGRRLE
                     FSSAGELAWLAKLDHEREQPLQYCAAAPILSGAGQVLGALAFYFPAPTISGPANREMM
                     ESASRLAAIAIEQRQLTDQLAHQAQHDPLTGLPNRLYLRSRLESALEQARINDSILAV
                     FFIDLDRFKQINDTLGHSTGDMLLQKVGERFQGRLRKADFLARMGGDEFTVLVSELRR
                     PEDAIIVGRKLLDALREPFEIQGTGFVVTASIGISFYPKDGTDTATLLSNADRAMYRA
                     KNLGKNDLECFTPELSGAALESLKLENDLRRALENGELILQYQPQRTLDGVLSGLEVL
                     LVWEHPRHGRIPPNVFIPLAEESGMIVSIGSWVLNHACLQAREWIDAGYPPVKIAVNV
                     SPLQFSRSDLVRTVSGALEASGLDPKLLELELTESVIMHNAEESMERMSRIRALGVGI
                     AIDDFGTGYSSLSYLRNLPVNTVKIDQSFLREIEAPGGAMALVGTIVSLAHQMGLSVV
                     AEGVESEEQLEMLRGAGCDKGQGNLFGEPLAPGRVHELLAQLTTTE"
     gene            complement(4514..>4698)
                     /locus_tag="VFQ79_13555"
     CDS             complement(4514..>4698)
                     /locus_tag="VFQ79_13555"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0140742.1"
                     /translation="RAVILMDGSLGSITVLNAAPSELAAAAVEAASQWRYQPTLMNGK
                     PVETETVIEMTYQLTP"
BASE COUNT          968 a         1399 c         1451 g          880 t
ORIGIN      
        1 tattggcgcc tggagcccta tattggcttt ggcgccgatg cgcattcctt cgatggcaag
       61 acgcggcggc ggaacgtgga aggcgccggc gaatatgtcg atagaatccg ccgcggcctg
      121 gatccctgcg ccgccgagac gccggctgag ctttccgagg agcgcttttt cgtcggcctt
      181 cggctggagg aaggtattcg cccggcgagt tccgaactcg cgcgatttgc agagcccatc
      241 cggcggttcc tcgatgacgg actgcttgcc atggaagacg ggcttctgcg tttgacgaac
      301 cggggagtcc tgctatcgaa cgaagtgttt caggaattcc tgacatgagc gaacagccgc
      361 tggtggatct tcgcagcgac accgtcacac gccccacggc atcgatgcgg cgcgcgatgg
      421 cggaagccga agtcggcgac gacgtttatc gcgaggatcc cacggtcaac cgtttggagg
      481 agcgtgccgc ggcgatgttc gcgaagcagg cggcgctgtt tgtcccgagc ggaacgatgg
      541 gaaacaccat cgccgtcaag cttcacaccg aacccggcca ggaagtcatc tgcgaggcca
      601 aagcccacat cgtgacctat gagctttcga tgctggcgtg gttctcgggc tgtctcgcgc
      661 ggacgattcc caccgcgcac ggcattctca cctgggagca gatccggcga gagatccgtc
      721 ccccggcgcc tttttgggct ccgaccgggt tgattgaaat tgagaacacg cacaacatgg
      781 ccggcggtac catctatccc atcgaagccg tcgacgagat ctgccacgag gctcatgccc
      841 tggggctgaa ggtgcacatg gacggagcgc gaatcttcaa cgccgctgcc gccctcggca
      901 tttccgtcgc tcgcatagcg gtgcacgtag acacggttat gttttgcctc tccaaagggc
      961 tcgccgcgcc tgttggatcg atgcttgtag gatcgcgcga cgacatggac cgggcacgtc
     1021 tccaccgcaa acggcttggc ggcggcatgc ggcaggcggg cgtgctggcc gccgcggctc
     1081 tggtcgcgat cgaggagatg acagaacggc tggtggacga tcacgcgaac gcggcgtttc
     1141 tggcggagga acttgccaac atccgcggca tctctctcga cccccgtcgc gtcgaaacca
     1201 acatcatcat tttcgacgtg agcggaaccg ggataacggc agccgatttc agcgcgtgtt
     1261 tgaaatcgcg cggtattctg atgaacccgg taagccccac ggaaatccgc gccgtcacgc
     1321 actgcgacgt cacccggcgg gattgcgaac gcgcgctcca aacgataacg gaaatcgcgc
     1381 ggagcgccgc tgcttccgcc tgaaagctac ctcaggaaaa cacgcagtcc ttgttgcagg
     1441 gccaccgcgc cagccgcgcc cgtcagcgcc ggcatgaacg caggcaccag accccggcgc
     1501 ttgaattcgc catggatggc atgaagcccg aggtagctga agctgccgcc gatcaccgcc
     1561 agtaacacac cgaaccatgc cgcggtcatc tgtccggtaa gcagcgattc gaaaccgccg
     1621 cccacgaaca tcgccgattc ggcagccgcg caccaggcca aggctttcca gggagactga
     1681 acggcggagc gcagaatgat gccgaacgcc agcccctcgg gaattttgtg cattgcgatg
     1741 gcaaagagca acatcgttcc cggaccggcc gtgtaggacg ccgcagcacc gaacccgtcc
     1801 agaaagctgt gggcggaggc ggcaacaatc atcggtgttg cgaagccgtg cagacgcgtg
     1861 atgcaaacgt catggtcatg ggaatgagag caggaagggc agaccggata cacaaagcgg
     1921 tctattaccc aaagagccag gaagccggcc aacagccata gcaggctggc gtaccattcc
     1981 agttcctcca ctacttccgg gacaatgccg aatgcacaaa taccgatcag caggccgccg
     2041 ctgaaaggaa ccagcttgcg ggatatcgca ggcatgctgg tcaggcgaat gccggcagcg
     2101 gccgccaatg tggcgaccag cgtcaacaga agtagtaaca tcaggagtga ttggggcact
     2161 ggaagtggct cgcttgaggc ctgattccac tgtaacgtac cgggacttgc gttaccaggg
     2221 atatgccggt ccttttctta cccggcctaa gtccagtcgt tcgccggatt acttagaacc
     2281 agtagaacta ctgaacagga gtccggtaat gttagtctga taatcggttg aggaccagtg
     2341 cagcagtacg gcgaacaaca gaccgccaat ctcagctccc agggggacct gtaccgggag
     2401 ctattcgaaa acgccaccga tatcatctac atacacgatc tggatgggcg cttcactgct
     2461 gtaaacaagg cgatcgaacg cattaccggt tattgccgcg ccgaaatact gcagatgtcg
     2521 ctggcagatc tcgccgttcc ggaataccag gagttggccc gccagattac atttgaagcg
     2581 ctgggcgggg gaacctccga gccggtcgag ttcgtcattc ggtcgaaagc cggaaagcgg
     2641 attgtgctgg aggttacttc gcgcctgtta tttcaggacg ggcgtccggt tggagtgcag
     2701 ggattcgccc gggatatttc cgcccgcagg cagcttgaac aggaccgcaa ccgtgtgctg
     2761 gagatggtgg ccaagaatat cgagctgccg gaagtactga acgagctgat gggaatggtc
     2821 cagcggcagc ggccgggaat tgccggcgcg gttcttcagc gccgcggcga tcgcctctat
     2881 gtgatagcag cgccggacct gcctccggcg tatttcgatc acatgcgtgg cacttacgcg
     2941 gaccggcgag gtggatgctg cggcgcagcc gtcgagggcc gccgcctgga attcagcagt
     3001 gccggcgaac tggcctggct tgccaagctg gatcatgagc gcgagcaacc gctccagtat
     3061 tgcgcggcag ccccgatttt atcgggtgcg ggtcaggttt taggggcgct cgcattctat
     3121 tttccggctc ctacaatatc ggggccggcc aaccgcgaaa tgatggaatc cgccagcagg
     3181 ctggcggcca ttgccatcga gcaacgccaa ctgacggacc agttggctca ccaggcgcag
     3241 cacgatcctc tcactgggct gcccaaccgc ctgtacctgc ggagccgcct cgaatcggct
     3301 ttggagcagg cacggatcaa tgattccatt ctggccgttt tttttatcga tctcgaccgc
     3361 ttcaagcaga tcaacgatac gctcggccat tcaaccggcg acatgcttct gcaaaaagtg
     3421 ggagaacggt tccaaggcag gttgcggaaa gctgattttc tggcgcgcat gggcggcgac
     3481 gaattcacgg ttctggtttc cgagctgcga aggccggaag acgccatcat cgtcggccgc
     3541 aagctgctgg atgcccttcg cgagcctttc gaaattcagg ggacggggtt tgtcgtcacc
     3601 gccagcattg gcatcagttt ctatcccaag gatggcaccg acacggccac gctgctgagc
     3661 aatgccgacc gggcgatgta tcgcgccaaa aacctgggca agaacgatct cgaatgcttc
     3721 actccggagt tgagcggggc cgcactcgag agcctgaagc tcgagaatga tttacggcgc
     3781 gcgctcgaaa acggcgagct catcctgcag tatcaaccgc agaggaccct cgatggcgtc
     3841 ctcagcgggc tggaagttct gctcgtttgg gaacatcccc ggcacggacg catcccgcca
     3901 aacgttttca ttccgctggc cgaggaaagc ggcatgatcg tctccatcgg ttcgtgggtt
     3961 ttgaaccacg cctgccttca ggcaagggag tggattgacg cgggctaccc gccggtgaag
     4021 atcgccgtca acgtctcgcc gctccaattc agccggtccg acctggtgag aacggtttcc
     4081 ggagcgctgg aggccagcgg cctggatccg aaactgctcg aactcgaatt aacggagagc
     4141 gtgatcatgc acaacgcgga ggagtcgatg gagaggatga gccggattcg cgccttgggc
     4201 gtcggcattg ccatcgacga tttcggaacc gggtattctt cccttagcta cctccgcaac
     4261 ctgcccgtca atacggtgaa gatcgatcaa tcgtttctgc gcgagatcga agcaccaggc
     4321 ggtgccatgg cccttgttgg gaccatcgtt tcgctcgctc atcaaatggg cctctcggta
     4381 gtagccgaag gagtggagag cgaggaacaa ttggagatgc tgcgcggcgc cggatgcgat
     4441 aaagggcaag ggaacctttt cggcgaaccc cttgcccccg gacgggtcca cgagcttctg
     4501 gctcagctca ccactacgga gtaagctggt acgtcatctc gatgacagtt tcggtttcca
     4561 ccggcttgcc gttcatcagc gtcggctggt acctccactg gctggccgcc tccacggcgg
     4621 cggctgccag ttcgctggga gcggcattta agaccgtaat gcttcccagg gaaccatcca
     4681 tcaggattac ggcgcgca
//
LOCUS       DASTMK010000310        15392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_175140, whole genome shotgun sequence.
ACCESSION   DASTMK010000310 DASTMK010000000
VERSION     DASTMK010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15392
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175140"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..979)
                     /locus_tag="VFQ79_13560"
     CDS             complement(128..979)
                     /locus_tag="VFQ79_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0140743.1"
                     /translation="MISRRGFLSAGAVLAVPCAFGAGRQPKIGVTDWNLRQAGKLEAV
                     SLARQLGFEGVEVSLGRNLVDGKLPLDNPEIQERYISEAKKNKVRLAGTCLDILHVNY
                     LKNDKLALKWIASGIPITKKLDAKVMLLPFFGKGALSTRQEMDYVGDALKELAPEAEK
                     AGIKLGLENTISAEDNVRIMDRAGSKAVLVYYDVGNSTGQGFDIIREMRWLGGRRICQ
                     IHLKDNPHYLGEGKIDFEKVMRTIRDIDFSGFANLETSSPSKSIEADMKRNLAFIRRL
                     MASVNRA"
     gene            complement(1015..2334)
                     /locus_tag="VFQ79_13565"
     CDS             complement(1015..2334)
                     /locus_tag="VFQ79_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0140744.1"
                     /translation="MQRLLVVEDDSAVRTTIVTFLEMEGYQVEAVSNTRDAIEKLRNG
                     DFPIVISDIYLDERTGLDVLEAARTSNPSCAVILMTARGTMETVMAATRGGAFDYIAK
                     PFELDQMLETVKRAAESISGGTDEDNSDVEDLPESEMIGSSPKMIAIYKVVSRVAPTD
                     ATILLEGETGTGKELVARMIHRNSARAEHPFVPVDCASIAPALLESELFGALKGAYTG
                     ADRDRMGVFEGANQGTVFLDEIGDIELNFQLKLLRFLQEREVRPLGSARARKVDVRVI
                     AATNRDLQKMVEEGKFREDLWFRLNVVRVTLPPLCERRGDLPLLAHFFLKKYNERYKQ
                     QARITETGLRAMEQYTWPGNVRQLQHLMERLTILAPNGRIDEHAVDMAIEASEPREQA
                     GETLAETEMDQIRRVLAATGGNKSRAAKILGIERKTLYRKLERMGLV"
     gene            complement(2390..4288)
                     /locus_tag="VFQ79_13570"
     CDS             complement(2390..4288)
                     /locus_tag="VFQ79_13570"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEU0140745.1"
                     /translation="MRRPDKPSAGYIAALAAVFLLAVAGGWTAPGTQIDNYAYDFMFR
                     LMASPPSTMDSVVLAFDDRTLRETGGMSQLRSVLADVLDLVSRQEPKAVAVDIILADT
                     GADQAAREADQRLAAAFRNVRHLVLPCDIVPGGWQDPAPAFRKTAEALGHVHGGADKL
                     DSVTRQIPLQAVANGQRRWALSLEAFRLSRSAGPLIESPDDIELDGARIPTSGPDRQM
                     RVRYVPPDAEGRSRISTVSVKDVLDKPETARLFRDKVVFIGVTSQSAARDRPLIPTSY
                     GQTTPGVEVHAHAYETLAGQQFFTTTGQLPVLGLCALLVTFAGLIFRYRDGWGAYSLG
                     GLLVACAMVLPFVLFSRNVVFPVFGPTASAWLSVGVAAAYQHFVIRRRLRRTEAERFR
                     YQQAIRFVTHEMKTPLTAIQGSSELIGRYNFTEEKRKQIAEVINSESKRLSGMIQTFL
                     NVERLSEGEMQLKQETFELRHLLEPSLARVAPLAERKQIAIVTGQTDNVLLNGDRELM
                     EYAVYNLLTNAIKYSPAATEVAVTAVRDGGVIRISVRDQGIGMDESDLRNIFRKFYRT
                     RRAEASGEAGTGIGLSIVEQIVVSHGGSIEVTSSPGKGSCFTLVVPATALPAGEVPGD
                     SMQERQIK"
     gene            complement(4272..5552)
                     /locus_tag="VFQ79_13575"
     CDS             complement(4272..5552)
                     /locus_tag="VFQ79_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEU0140746.1"
                     /translation="MRWIAWGILLTLLTPAATKPDHSATDEYRSRRANLREAITDGVI
                     ILEGNTEEAGDLRTGFLQEPNFYYLTGWAEPGAILLMTPGDEVLFVPRRNPEAEKWTG
                     KKASPDDRDIQKRTGFKTVLSTESFESQVVRYLEAAPKVYLLKGKPAAARLERLLAMR
                     SIQDAAPAIARLRMVKSAQEQDLIRRSIDVSIHGHRAIWQRAAAGLHEYQLAATLTSV
                     FLESGCTGPAYAHIVGSGPNATVLHYSTNNRRMDSGDLLLVDAGAECSGYAADITRTI
                     PVNGKFTSRQKEIYNIVLGAQNAAIAAARPGATIGKTTANSLYKVAYDYIDSHGKDKQ
                     GNTLGKYFTHGVSHHVGLEVHDANDPAMPLAPGMIITVEPGIYLPEENIGVRIEDMIL
                     ITENGAKVLTEDLPKDIEAIEQALAQRKSYAPSR"
     gene            5675..5983
                     /locus_tag="VFQ79_13580"
     CDS             5675..5983
                     /locus_tag="VFQ79_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU0140747.1"
                     /translation="MKRLFVGNVPFRAKEDELQDWFNQSGIDVDSVHLIRDRASGEPR
                     GFGFVEVSDDEQAERAVQACNGSSFMGRSLIVNEARPRGSGPVSGQSMGAGRSGGMVS
                     "
     gene            complement(6004..6972)
                     /locus_tag="VFQ79_13585"
     CDS             complement(6004..6972)
                     /locus_tag="VFQ79_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0140748.1"
                     /translation="MEKITLKRTDLTVSRVCFGTMTFGSQVDQAGAEAIVNRCLDAGV
                     NFFDTANIYNKGTSEVMLGKALGQKRNGIVLASKVFGKMGDAPNESGLSRTAILHAVE
                     GSLKRLGTDYLDIYYMHQPDYSVPMEESLEAMDRLVRDGKVLYPASSNYSSWQVTRML
                     WIAEASGYAPPYISQPMYNLLARGIEQEFLPMAKEMDVSTVIYNPLAGGLLTGKHKPE
                     APLPGTRFDDNQMYLDRYWHPAQFEAVAELGATARQAGRSLVSLALNWLLHHTPADCV
                     ILGASSLQQIEENFSASEEGALPEDVLGECDRVWAKLRGITPRYNR"
     gene            complement(7011..7979)
                     /locus_tag="VFQ79_13590"
     CDS             complement(7011..7979)
                     /locus_tag="VFQ79_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140749.1"
                     /translation="MPKFASSLFLTMLLGIPAPAVDTTLLNTVMPDARVVAGVNARQI
                     KASDFGKFLLSQSEGEDRKFQEFILETGLDPRRDIDEILVSSKDGKHGSGILAVRGLF
                     NTQQILSAVARKGGIATKYNDADLISRKADSKSVVAIVNGTFAIAGDAGEVRAALDRR
                     RAAMPAVDAATVRKLDGLSARYDAWVFVRSPADLAQGHARRDGGARLNAQSLEAVEEI
                     SSGIEFGPSVRISGEALARSDKDATALADVLRFVAGLVQLRSDNPGPQALADLLNSLE
                     IKTEANRLTFSMAIPETQLEEMIRSHRTAPARPHRPNRPNRPARPA"
     gene            complement(8055..8729)
                     /locus_tag="VFQ79_13595"
     CDS             complement(8055..8729)
                     /locus_tag="VFQ79_13595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0140750.1"
                     /translation="MVVRFLGFLVFGLMTCSAGVITEGFTFSVASAGGDHSRGTHFHS
                     STGGVYGDPDGKAEVGSYASEEVRGLSEYNLAGLGMLDSVFVTFNIYSAGGLSDGVND
                     EPWTGTINVYAYQGNNGESVTDYEVLPIAFAGVLSTSGLVEGGIVSLPITSIFNSAIG
                     GGLASLGIRLERAGNVPSYSGAWTFDTFRLTSDDQSTAFTPEPHTYLLTAAGLVGLAF
                     ARRRRK"
     gene            complement(8904..10259)
                     /locus_tag="VFQ79_13600"
     CDS             complement(8904..10259)
                     /locus_tag="VFQ79_13600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0140751.1"
                     /translation="MKRIVLPLAAIVVAAVILLFVLSSATALRVDPPVRVVGTETPVR
                     LQASNPHGIRRVTAWIEQNGQQHQVFEKAESATRFFFFGRNEPAHEITFSVGTKDAPS
                     VKDGKARLIIETTANDFRSATDRLVSDVEINTRPPALTVDGHQHYINQGGSELVTFTV
                     SGYWTEAGVRVGKHTFRSFPMPGKDESSGERFSLFAFPWDTPADTVPVVYARNPSGAE
                     VTGRFWFKLFPKKFRSRELPLSDAFLQKVVNELDPGGSGDLLSRYLKINGEIRRQNNQ
                     TLADLRHKTEQKVLWSGPFVQLSNSKVESLFADTRSYMYNGRKVDEQTHLGFDLSVVK
                     NVPVVASNDGRVLYAAPLGIYGNCVVIDHGYGLQSIYGHLNSIGVKPGELVKKNQEIG
                     RSGETGLAGGDHLHFSMQVDGVQVNPIEWWDDHWIQDRILSKLTPSGGEVSRARDTYT
                     P"
     gene            complement(10352..11785)
                     /locus_tag="VFQ79_13605"
     CDS             complement(10352..11785)
                     /locus_tag="VFQ79_13605"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU0140752.1"
                     /translation="MSRIFISYRREDAAGYAISIHGCLADRFGTEALFMDLGSIEPGE
                     DFIQAITEKVGGCEVLIVVIGRMWLTCADEDGRRRLEDPTDFVRGEVSAALQRKIRVI
                     PVLVGGASLPKPDQLVPELAPLCRRQAITLTDSAFWQDAGRLVESIEKVIGRSGNPPR
                     PASAATRAVSRKTSLAAREARRQRPAAPVSPTNRIRFGRERIFRAHRKPVKTVAFSPD
                     SQILASAGGGSWLGGGDTTIRLWRVADGRLLREMAGHNDTVSKVVFSPDGLRLASCCS
                     SAINIWRLSDGKLLRALTGNETGGGDSLAFSNDSSRLACWVAGNSGENTDTTYDVWSV
                     PKGKHLGTYFLSRLPGRGEFQWEHASSGRRMIASKEFIRIEEDAYWAIVVKAAKRGTV
                     PLELRDNDNAEPGEWMFCPGGRSLLAACKDNVIRLWRLEDGARIARMQVSSVPNSISP
                     DQQWVAVGCEDKTVRLWPVLRHSSTQV"
     gene            complement(11836..12468)
                     /locus_tag="VFQ79_13610"
     CDS             complement(11836..12468)
                     /locus_tag="VFQ79_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0140753.1"
                     /translation="MIHEILPVGMLQCNCSIFGDEQTRDALVVDPGDDIEAILGILQQ
                     HSLKVKAIVITHAHIDHIGGAAKLKTVTGAPVYMNERDEELNRQMDMQAAWLGMEVPE
                     RAAVDVPLRDGDRITLGATSFEVLHTPGHTQGSISLWIPAEQKVIAGDTLFRDSIGRT
                     DLPGGDGVQILASIRDQLLWLPPDAVVFPGHGPSTTIGREKEFNYFLRGI"
     gene            12494..12940
                     /locus_tag="VFQ79_13615"
     CDS             12494..12940
                     /locus_tag="VFQ79_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140754.1"
                     /translation="MDPTQTLLGVLMRWIHITSVVFVVGGMLYARLILLPAMARLSPQ
                     DRQSMTDRLAAYLSPWAWAAMAAIVGSGLYNFFTKAGYPPGYHMWFGIKILLALHVFA
                     VLMLLTKSGGDERKRARWMTGVIISALIVIAISAWLRWISVPAVPA"
     gene            12937..13890
                     /locus_tag="VFQ79_13620"
     CDS             12937..13890
                     /locus_tag="VFQ79_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEU0140755.1"
                     /translation="MTEQGYKAFRESAALLDLSGRGKLRASGEDRARLLHAMTTNHIQ
                     EMQPGQGRYVFFLNAQGRIQCDANMLAMADHFLIDTEPETHEKLYAHLDSYIIADDVV
                     LEDLTVATATLSIEGPHAERILSAIGAPVPGEPMASVAWSAKLAARLNSTGMPGYFLM
                     VPAEEKDALVDELVKAGAVPADSEDARAVRMENGKPRYGEDILETVIPQETQVMYSVH
                     FNKGCYLGQEIVERVRSRGRLNRMLVSLDIETQTPPPANTKITVDEKEAGRITSAVFS
                     PNRGKVVAFAYLRAERVEATNLMVEGAPAGGVRCPLNQPET"
     gene            complement(13875..14732)
                     /locus_tag="VFQ79_13625"
     CDS             complement(13875..14732)
                     /locus_tag="VFQ79_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0140756.1"
                     /translation="MIDLHTHTYESDGTYSPRELVEAAIEAGLEALAISDHDTFSGYD
                     QAVLIARDVGLDLVCGIELSTKHAFEATSKSKSVHLLAYFFNGGPPADFREWLVEMQS
                     NRRDRNRRLAARLRDFGVDIKLEEVEALGRTMTGRPHFARVLLEKGYVSSYDEAFRLY
                     LDESAKAYVDRIQPALLDAIQRIKTFGGISSLAHPVRLGRRDVQKEEAFVALLRDAGL
                     DAIEVWHSDHTEEDTRRYLGLAEKYDLAVTGGSDFHGAVKPSVRLGVGRGQVATPKEA
                     LDRLRQVSG"
     gene            complement(14729..15277)
                     /gene="cysC"
                     /locus_tag="VFQ79_13630"
     CDS             complement(14729..15277)
                     /gene="cysC"
                     /locus_tag="VFQ79_13630"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF002059.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEU0140757.1"
                     /translation="MHKGFTLWFTGLSGAGKSTISHAIYTRLKELGARVELLDGDVVR
                     TNLSQGLGFSKEDRDTNIRRIGFVCELLSRNGVIALVAAISPYRAVREELKTRIANFL
                     EVHVDCPVEVLAERDVKGLYKKALAGEIPNFTGVSDPYEPPLQPDIVVDTSRESLEES
                     AAKVWTKLEELGLLSFSREQVH"
BASE COUNT         3050 a         4607 c         4459 g         3276 t
ORIGIN      
        1 cttggatgga cgatatttgc tgaatacgta gcagtgctac cagataagga aaagagcaga
       61 caatccgcaa gacatactta ccacctcagg aaggtggtcg tccgataaaa tgtgccgtcc
      121 agaccgttca ggcccggttt acgcttgcca tcagtcgccg gatgaaggcg agatttcgct
      181 tcatgtccgc ttcaatggac ttggacggag aactggtttc caaattcgca aaaccgctaa
      241 aatcgatgtc ccggatcgtc ctcattactt tctcaaaatc gatcttgccc tcaccgaggt
      301 aatgagggtt atctttcagg tgtatctggc atatgcggcg gccgccgagc cagcgcatct
      361 cccgaataat gtcgaagccc tgccccgtgg aattcccgac gtcgtaatat accagcaccg
      421 ccttggagcc ggcccggtcc atgatccgga cgttatcctc ggccgatatc gtgttctcca
      481 ggcccagttt gattccagcc ttctccgctt ccggcgccag ttctttcagc gcgtcgccca
      541 catagtccat ttcctgccgc gtcgacagcg cgcctttgcc gaagaacgga agcaacatta
      601 ctttggcatc caacttcttt gtgatgggaa taccgctggc gatccatttc agcgcgagtt
      661 tatcgttctt tagatagttg acgtggagaa tatcgaggca ggtgcctgcc agccgcacct
      721 tgtttttctt tgcttcggag atgtagcgtt cctggatttc cggattatcg agtggcagtt
      781 tgccatccac caggttgcgg ccaagactga cttcgacccc ctcgaatcca agctggcgcg
      841 ccagcgatac ggcttcgagc tttccggctt gccggagatt ccaatccgta acaccgattt
      901 tcggctggcg cccggcgccg aacgcgcacg ggacggccag gacggctcct gccgagagaa
      961 atcctctgcg cgagatcatt cctcctccaa agctcgattc aaatgtacct ggaattatac
     1021 cagacccata cgttccagtt tgcggtaaag tgttttacgc tcgattccaa ggattttcgc
     1081 ggcgcggctc ttgtttccac ccgtggccgc cagcacccgg cgaatctggt ccatctccgt
     1141 ctcggccagc gtctcgccgg cctgttcgcg cggctcgctt gcttcgattg ccatgtcaac
     1201 cgcgtgctcg tcgatgcgtc cgttcggcgc caggatggtc aggcgctcca tcaggtgctg
     1261 caactgccgc acattgcccg gccaggtata ctgctccatc gcgcgaagcc cggtttccgt
     1321 aatccgcgcc tgctgtttat accgctcgtt gtactttttc aggaagaagt gtgccagcag
     1381 cggcagatcg ccgcgtcgtt cgcaaagtgg aggcagcgtc acgcgcacca cgttcaggcg
     1441 aaaccacaaa tcctcgcgga atttgccttc ttccaccatc ttctggagat cgcggttcgt
     1501 agcggcgatg acacgcacgt ccacttttcg cgcgcgggcc gagccgagcg gacgcacttc
     1561 ccgctcttgc aggaaacgta gcagcttcag ttggaaattg agctcaatat cgccgatttc
     1621 atccagaaaa accgtaccct gatttgcccc ttcgaacacg cccatgcggt cgcggtcggc
     1681 gcccgtataa gcgcccttga gagcgccgaa cagttcgctt tcgagcagcg ccggagcgat
     1741 cgaggcgcaa tcgaccggaa cgaacgggtg ttcggcacgc gcgctattgc gatgaatcat
     1801 gcgtgcgacg agttcttttc cggtccctgt ttctccttca aggagaatcg tagcgtcggt
     1861 aggcgcaacg cgggacacca ctttataaat agcgatcatt ttcggcgaac tgccgatcat
     1921 ctcgctttcc ggaaggtcct ccacgtcgct attgtcttcg tccgtgccgc ctgaaatgct
     1981 ctccgcggcc ctcttgaccg tttcaagcat ctgatccaat tcgaagggtt ttgcgatgta
     2041 gtcgaaggcg ccgccgcggg tcgccgccat cacggtttcc atcgttccgc gcgcggtcat
     2101 cagaataacc gcgcaggagg gattgctcgt ccgtgccgct tcgagcacat caagtccggt
     2161 tcgctcgtcc agatagatat cggaaataac aatcggaaag tcgccgttcc gtaatttctc
     2221 gatggcatcc cgtgtgttgg agacggcttc gacctgatag ccttccatct cgagaaaagt
     2281 gacaatcgtc gtgcggacgg cggaatcgtc ttcgaccacc aacaaacgct gcaaacgaac
     2341 tccctaaaac aataaaaagc gagaaatatc gaggatgtaa acgttcattt tacttgattt
     2401 gccgctcttg catagaatcc cccgggactt caccggccgg caaagccgtt gcgggcacca
     2461 ccagtgtgaa gcacgagcct ttacccggac tgctggtcac ttcaatggag cccccatggc
     2521 tgacgacgat ctgctccaca atcgagagcc cgattcctgt gcctgcttct ccggaagctt
     2581 cagcccttct ggtgcgatag aacttccgga agatgttccg caaatcgctt tcatccatgc
     2641 cgatgccctg atcgcgaacg gaaatccgga tgactccccc atcccgtacg gccgtgacgg
     2701 cgacctctgt cgcagcgggc gagtatttga tggcattggt caacaggttg tagacggcgt
     2761 actccatcag ctcgcggtct ccgttcaaca gcacgttgtc ggtctgtccg gtcacaatcg
     2821 cgatctgctt gcgctcggcc agcggagcca cgcgggccag cgacggctcg agcaaatggc
     2881 gcaattcgaa cgtttcctgc ttgagctgca tctcgccttc ggaaagacgc tcgacgttga
     2941 gaaacgtctg gatcatgccg gagagccgct tcgattccga gttaatgact tctgcgatct
     3001 gctttcgttt ttcctcggtg aaattgtacc ggccgatcag ctcgcttgag ccctgaatgg
     3061 ccgtgagcgg agttttcatc tcgtgtgtca cgaaccggat cgcctgttga taccggaagc
     3121 gttccgcctc ggtccggcga agcctgcgcc ggataacgaa atgttggtag gcggcggcca
     3181 ctccgacact gagccaggcc gatgccgtgg ggccgaaaac cggaaagacg acgttgcggg
     3241 agaaaaggac aaacggaagc accatggcgc aggccaccag cagccctccc agcgagtatg
     3301 ctccccatcc gtcccggtac ctgaaaatca atccggcgaa cgtcaccagc agcgcgcaca
     3361 atccgaggac cgggagttga ccggtcgtcg tgaagaattg ctgcccggcg agtgtttcat
     3421 aggcgtgcgc gtgcacttcc acgccgggcg tggtctgccc gtaagaggtc gggatcaatg
     3481 gacggtcccg tgcggcggac tgggaagtta caccgataaa caccactttg tcccggaaca
     3541 gccgcgccgt ttcgggcttg tcgagcacat ccttcacgga gacggtggag atccgggagc
     3601 ggccttccgc atccggcgga acatacctca ccctcatttg ccggtccggg ccggatgttg
     3661 gaatgcgagc tccgtccagc tcgatatcat cgggggactc tatcagaggt cccgccgagc
     3721 gggaaaggcg gaaggcttcg agcgatagcg cccagcggcg ctggccattg gccaccgcct
     3781 gcaacggaat ctggcgtgtt acggagtcca gtttgtccgc tccgccgtgc acatgaccaa
     3841 gtgcttcggc tgtcttgcga aacgccggcg cagggtcctg ccaaccgccg ggcacgatat
     3901 cgcagggcag cacgaggtgg cgcacgtttc gaaaggctgc cgccagacgc tggtcagctt
     3961 cccgggccgc ctggtccgcg ccagtgtcgg cgagaatgat atcgaccgcc accgctttcg
     4021 gttcctggcg cgagacaagg tccaggacat cggcaagcac agatcgtagc tgactcatgc
     4081 cgcccgtctc gcgcagcgta cggtcgtcga aagcaagcac cacgctgtcc atggtggagg
     4141 gcggggaagc catcaaccgg aacatgaagt cgtacgcgta gttgtcgatc tgcgttccgg
     4201 gtgcggtcca tcccccggcg acggccagca gaaacacggc cgccagcgcc gcaatgtaac
     4261 cggcagacgg cttatcggga cggcgcataa gacttccgtt gagccagggc ctgttcgatg
     4321 gcttcgatgt cttttggcag atcctccgtc agaaccttgg cgccgttctc cgtaatgagg
     4381 atcatgtcct caatgcggac accgatattc tcttcgggca ggtagatgcc aggctccacc
     4441 gtgatgatca tgccgggcgc cagcggcatg gccggatcgt tcgcgtcgtg aacctccagg
     4501 ccaacgtgat gactgacgcc gtgggtgaag tattttccca gtgtgttgcc ctgcttgtct
     4561 ttcccgtgcg agtcaatata gtcgtaggcg actttataca ggctgttggc tgtggttttt
     4621 ccgatggtcg cgcccggccg cgccgccgcg atggcagcgt tctgcgctcc gagcacgatg
     4681 ttgtagatct ccttttgccg gctcgtaaac ttgccgttta ccggaatggt gcgtgtaatg
     4741 tcggctgcgt agccactgca ctcagcgccc gcatccacca gcagcagatc gcccgaatcc
     4801 atgcgccggt tgttggtcga ataatgcagg actgtggcat tgggtccgga gccgacgatg
     4861 tgcgcgtacg cgggaccggt gcagccactt tcaaggaata cggaagtcag cgtggccgcg
     4921 agttgatact catgcaggcc cgctgctgcg cgctgccaga tagcacgatg gccatgaatg
     4981 ctgacgtcga tcgaccggcg gatcaggtcc tgttcctgtg cggacttgac catccgcagc
     5041 ctggcgatcg cgggcgcggc atcctgaatg gaacgcatcg ccagcaggcg ttcgagccgt
     5101 gccgctgcgg gctttccctt gagaagatac accttcgggg cggcttcgag atagcgaacc
     5161 acttgcgact cgaaggactc cgttgacagc accgtcttga aaccggtgcg cttctgtata
     5221 tcccggtcat cgggagacgc ctttttgcca gtccactttt cggcctccgg attccggcgt
     5281 ggcacgaaca aaacttcgtc cccaggcgtc atcagcagga tggctccggg ctccgcccac
     5341 ccggttagat aataaaaatt cggctcttgc aaaaaacctg tgcggaggtc gccggcctct
     5401 tcggtattcc cctcaaggat gatcacgccg tccgtgatcg cttccctcaa atttgcacgg
     5461 cggctacgat actcgtctgt ggcggaatgg tcgggtttcg tcgctgccgg tgtgagcagc
     5521 gtcagcagga taccccaggc aatccaacgc attcagtaca agtatagccg ccctgttccg
     5581 gctggcgacc cgggagagtg aaacgtaaat aaccgttaca agcttgtaca tgaaagcggt
     5641 tctccttaag gtggagaagt ctaggagtga tggcgtgaag agattattcg ttggaaatgt
     5701 accgtttcgg gccaaagaag atgaattgca ggattggttt aaccaatccg gcatcgatgt
     5761 agattccgtt catctgatcc gcgaccgggc gtccggagaa ccaagggggt tcggattcgt
     5821 ggaagtctcc gatgatgaac aggcggagcg agccgtacaa gcctgtaatg gatcgagctt
     5881 tatgggacgc agcttgatcg tgaacgaggc gcgccctcgc ggcagtggtc ctgtctcggg
     5941 gcagagcatg ggggccggcc gttccggcgg tatggtctca taacgaaacc gcggtttcga
     6001 gaattatcga ttgtatctcg gcgttatccc cctcaacttc gcccacaccc ggtcacattc
     6061 gccgagcaca tcttcgggca gtgctccctc ctccgaagcg ctgaagtttt cctcgatttg
     6121 ctggaggctg gacgcgccca ggatgacgca atctgccggc gtgtgatgaa gaagccagtt
     6181 caaggcaagg ctgacgagtg atcgtcccgc ctgccgggct gtcgcgccga gttccgcgac
     6241 ggcttcgaac tgagcggggt gccaatagcg atcgagatac atctggttgt cgtcgaaccg
     6301 cgtccctggc agcggcgctt ccggcttgtg tttgcctgtg agcagcccgc ccgcgagcgg
     6361 gttgtagatc accgtggaaa cgtccatctc cttcgccatg gggagaaact cctgctcgat
     6421 tccacgtgcc agcaggttgt acatcggctg gctgatatag ggcggggcgt agccggaagc
     6481 ttccgcaatc cacagcattc gggtgacctg ccaggagctg tagttggaac ttgcgggata
     6541 aagcaccttc ccatcgcgca caagccggtc catcgcctcc agcgattcct ccatcgggac
     6601 cgagtagtcc ggctggtgca tgtagtagat gtcgaggtaa tccgtgccga gccgttttag
     6661 cgagccttcc accgcgtgaa ggatggcggt tcgcgaaagt ccgctttcgt tcggcgcgtc
     6721 gcccattttg ccgaagacct tgctcgccag cacgatcccg tttcgcttct gcccgagggc
     6781 ctttccgagc atgacctcgg aggtcccttt gttgtagata ttggcggtat cgaagaaatt
     6841 aacgccggca tccaggcagc gatttacgat ggcttccgca cccgcctgat ctacctggct
     6901 tccaaatgtc atcgtgccaa agcacacgcg ggaaaccgtc aggtcggtcc gcttcagggt
     6961 aattttttcc attggctgcg attgtagcag cccgtggacg cccctcgtcg tcaggcgggc
     7021 cgggcggggc ggttcgggcg attcggccga tggggacgtg cgggcgccgt gcggtgactg
     7081 cggatcatct cttcaagctg tgtttcggga atggccatgg aaaacgtcag ccggttcgct
     7141 tcggtcttga tttccaggga gttcagcaga tccgccagcg cctgagggcc ggggttatcg
     7201 gaacgcaact gcaccaggcc ggcaacgaac cgcaaaacgt cagccagggc ggtagcgtcc
     7261 ttatccgagc gcgcaagggc ttcgccagag attctcaccg acgggccgaa ttcaatcccg
     7321 gaactgattt cctcaacggc ttcaagcgac tgggcgttca ggcgagcccc tccgtcgcgg
     7381 cgggcatgcc cctgagccag atcggcggga cttctcacaa acacccacgc atcataccgg
     7441 gcgctcaaac cgtccagttt ccgcactgta gcggcatcca cggccggcat ggccgcccgg
     7501 cggcggtcca gcgcggcacg cacttccccg gcgtctcccg caatggcgaa cgtgccgttc
     7561 acaatggcga ctacgctttt gctatccgcc ttccgggaaa tgagatcggc gtcgttgtac
     7621 ttcgtcgcga tgccgccttt gcgagcgacc gcggagagga tctgctgcgt gttgaagagg
     7681 ccccggaccg ccaggattcc gcttccgtgt tttccgtcct tcgaggacac cagaatttca
     7741 tcaatgtcgc ggcgtgggtc caagcctgtt tccagaataa attcctgaaa cttccggtcc
     7801 tcgccttcgg attgcgacag caggaattta ccgaaatccg aagctttgat ttgccgggca
     7861 ttcacaccgg ctacgacccg ggcgtcgggc atgacggtgt tcagcagcgt tgtatcgacc
     7921 gccggcgccg ggatacccag aagcatcgtc aaaaacaggg agctcgcaaa ttttggcatt
     7981 ttggtgtctc tcttaattat gcgaacgtca cctggagtgg aaaagttcca ctaactttgc
     8041 ggcgcgaaga ggcgctattt ccgccggcgt ctcgcaaaag cgagcccgac caatccggcg
     8101 gctgtaagca ggtacgtgtg gggttcaggg gtgaatgcgg tggactggtc atcactggtg
     8161 agccgaaagg tgtcgaacgt ccaggcccca ctgtaggatg gtacgttccc tgcgcgctcg
     8221 agtcgtatgc ccagcgaggc taggccgcct ccaatggcgc tgttaaagat cgatgtgatg
     8281 ggcagactga cgataccgcc ctctaccagg cccgacgtac tcaacacccc ggcgaaagca
     8341 atcggaagga cttcgtagtc cgtgacgctt tctccgttgt tgccttggta cgcgtaaaca
     8401 ttaatggtgc ccgtccaggg ctcgtcattc actccgtcgg acaggccgcc tgcgctgtag
     8461 atattaaaag tgacgaaaac ggaatcgagc atgccgagtc cggcgaggtt gtactcggag
     8521 agcccgcgaa cttcttcgga cgcataactt cccacttccg ccttcccgtc aggatctccg
     8581 taaacgcctc cggtgctgga atggaaatgc gtgcctcgcg agtggtcgcc cccggcggag
     8641 gcaaccgaaa aggtaaaacc ttcggtgatg acgccggcgc tgcatgtcat gagaccgaaa
     8701 accagaaacc ccaggaagcg cactaccatg gacatcctcc atctaaaata tcggcaatat
     8761 cggcgtcgct tgccaagaag ctgtgaagaa attgaattca gccacggatg aacacggatg
     8821 aacacagata agtttcctgt tttcaatctg tgtttatctg tgtccatctg tggctctgaa
     8881 tctttcacgc attctaacag ctcttatgga gtatatgtgt cgcgcgcgcg cgaaacttct
     8941 ccgcctgacg gagtaagttt gctgaggatc cggtcctgaa tccagtgatc atcccaccat
     9001 tcgatgggat tcacctgtac tccatccacc tgcatgctga agtgcaggtg gtcgccgccc
     9061 gccaggccgg tttcaccgct ccgcccgatc tcctggttct ttttcacaag ctctccgggt
     9121 tttaccccga ttgagttcaa atgcccgtaa atggattgca gcccgtaccc gtgatcaatc
     9181 acgacacaat tgccgtaaat accgagcggc gctgcataga gtacccgccc atcatttgat
     9241 gcgacaaccg gaacgttttt gaccacggac aggtcgaatc cgagatgggt ctgttcgtcc
     9301 actttgcgcc cgttgtacat gtagctgcgg gtatcggcga agagcgattc cactttcgaa
     9361 ttgctcaact ggacaaaggg acccgaccac agaaccttct gctcggtttt gtggcgcaga
     9421 tcggcgagag tctggttgtt ttgccggcgt atctcgccgt taatcttgag atatcgcgat
     9481 agcaaatcgc ccgagccgcc cgggtcgagc tcgtttacca ctttttgcag gaacgcgtcg
     9541 ctcagcggga gttcgcgcga acgaaacttc ttggggaata acttgaacca gaagcgcccg
     9601 gtgacctcgg cgcccgaagg gtttcgcgcg tacacaacgg gaacggtgtc cgcgggtgtg
     9661 tcccacggaa acgcgaacag cgagaagcgt tcaccgctgg actcgtcttt gcccggcatc
     9721 gggaaactcc ggaaggtatg cttgcccact cggacgccag cctcggtcca gtatccggag
     9781 acggtgaagg tgaccagctc ggagccgccc tggttgatgt agtgctgatg cccgtccact
     9841 gtcaacgcag gcggacgggt gttgatctcg acatcggaaa ccaggcgatc cgtcgcactg
     9901 cggaaatcat tggctgttgt ctcgatgatc agccgggcct tcccgtcctt caccgatgga
     9961 gcgtccttcg tcccgaccga aaacgtaatc tcatgcgcag gctcgttccg gccgaaaaag
    10021 aagaaccttg tggcggattc cgccttttcg aatacctggt gctgttggcc gttctgttcg
    10081 atccatgccg tcacacgccg gatgccgtgc ggattgctcg cctggagacg cacaggagtt
    10141 tccgtaccga ctacgcgcac gggaggatcg acacgaagcg ccgtagccga ggacaggaca
    10201 aataacagga tcaccgcggc aacaacgata gcggctaagg gaagaacgat tcgtttcatc
    10261 agactgctac cagcgtaacc cggctggctc gtgagccgcc tgcccgcttt tttgtgaccc
    10321 ttatcgcaag tgcccgcagc ggcgaagatc gtcaaacctg ggtactcgaa tggcgcaata
    10381 ccggccacag ccggacagtc ttgtcctcac atcccacggc aacccattgt tgatctggag
    10441 atatgctgtt cggtactgaa gaaacctgca tcctggcgat tctggccccg tcctctaacc
    10501 tccacaaccg gattacgttg tctttgcagg ctgcaagcag cgaccggccg ccgggacaaa
    10561 acatccattc tcccggttct gcattatcgt tgtccctcag ttcgagaggc actgttccgc
    10621 gcttggccgc tttaacgaca atggcccaat acgcatcttc ctcgatgcgt atgaactctt
    10681 tggaagctat catgcgacgg cctgatgacg cgtgctccca ctggaactcc ccgcggccgg
    10741 gtaaacggct aagaaaatag gtgccgaggt gcttgccctt cggaactgac cagacgtcgt
    10801 acgttgtgtc tgtattttct ccggaattac ccgccaccca gcaggctaac cggctgctat
    10861 cgttcgagaa tgcaagagag tcgccgcccc ccgtctcatt gccggtgagc gccctcagca
    10921 gttttccatc ggatagtcgc cagatattga tggccgaaga acagcacgaa gcgagccgga
    10981 gaccgtcagg ggaaaacacg actttcgaaa cggtatcgtt atggccagcc atttcgcgca
    11041 gcagccgtcc atccgcgacg cgccagagcc tgatggtagt gtctcctccg ccgagccaac
    11101 tgccgccgcc cgccgaagcc agtatctggc tatcgggcga aaacgccacg gttttcaccg
    11161 gctttcggtg ggcacgaaaa atacgctcac ggccgaacct gattctgttc gtgggcgaaa
    11221 ccggcgccgc tggacgttgg cggcgcgctt cacgtgcagc aaggctggtt ttcctggaga
    11281 ctgcccttgt cgcagcgctg gcagggcggg gcggatttcc ggatctgcct atcacctttt
    11341 cgatgctttc taccaggcga ccggcatcct gccagaatgc gctatcggtc agagtaatag
    11401 cctgccggcg gcacaaaggc gccaactcag gaaccaactg atccggcttc ggcaggcttg
    11461 caccgccgac cagcacgggg atgacgcgga tctttcgttg cagcgcggct gagacttctc
    11521 cacgcacgaa atccgtggga tcttcgagcc gccgccggcc atcctcgtcc gcgcaggtga
    11581 gccacattcg cccaattaca acaatgagca cctcgcagcc gccaactttt tcggtgatcg
    11641 cttggatgaa gtcttccccc ggctcgatgc tgccgaggtc catgaagagc gcctctgtac
    11701 cgaatcgatc ggcaagacag ccgtgaatac tgatcgcgta cccggccgca tcctcgcgcc
    11761 ggtagctgat aaaaatcctg gacacagctc aatatatccg gcagatttta tcacggctga
    11821 agagacgacg gctggctata ttccccgaag gaaatagtta aactccttct cgcgcccgat
    11881 ggttgtggag gggccatgcc cgggaaatac caccgcatcc ggtggcagcc acagcagttg
    11941 atcgcggatg gaggcgagga tctggacacc atcgccgccc ggtaaatccg ttcgcccgat
    12001 gctgtcgcga aacagcgtat cgccggcaat cactttttgc tcagccggaa tccagaggct
    12061 gatgctgcct tgcgtgtggc ccggtgtgtg aagcacctcg aagcttgtag cgccgagcgt
    12121 aatcctatcc ccatcgcgga ggggaacgtc cacggcggcg cgctccggaa cttccattcc
    12181 gagccaggct gcctgcatgt ccatctggcg attcaactcc tcgtcgcgct cgttcatgta
    12241 gaccggcgca ccggtgaccg tcttcaattt cgccgcgccg ccgatgtggt cgatgtgggc
    12301 gtgagtaatc acgatggcct tgaccttcag actgtgctgc tgcaaaatcc ccaggatcgc
    12361 ttcgatgtca tcgccagggt ccacgaccag tgcatcccgc gtctgctcgt ctccgaagat
    12421 ggaacagttg cattgcagca ttcccaccgg cagaatttcg tggatcatat aagcccgatt
    12481 gtatagtagt gatatggatc ccacccaaac cctgctcggt gttctcatgc gatggattca
    12541 cattacttcg gttgtgttcg tggttggggg tatgttgtac gcgcgtctga ttctgttgcc
    12601 cgccatggcc cgtttgagcc cccaggaccg gcagagcatg acggaccggc tggccgcgta
    12661 tttatcaccg tgggcatggg cggcaatggc tgccatcgtg ggttcgggtt tatacaactt
    12721 tttcacgaaa gccggatatc ctccgggtta tcacatgtgg ttcggtatca agatcctgct
    12781 ggcgctccat gtttttgcgg ttctgatgct gttgacgaaa tccggcggcg atgagcggaa
    12841 acgcgcccgg tggatgaccg gagtgatcat ctccgcgctg atcgtaattg cgatctccgc
    12901 ctggctgcgg tggatttctg ttccggcggt gccggcatga cggaacaggg atataaagct
    12961 tttcgcgaga gtgcggccct tctcgattta tccggtcgcg gtaagctgcg ggcttccggg
    13021 gaagaccgcg cgcggctgct ccatgcgatg actacgaacc acattcagga gatgcagccg
    13081 gggcagggga gatacgtctt tttcctgaac gcgcaggggc gcatccagtg cgacgcgaac
    13141 atgctcgcga tggcggatca cttcctgatc gacacggaac cggaaacgca cgaaaaactg
    13201 tacgcgcacc tcgacagcta catcatcgcg gatgatgtgg tgctggaaga cctcacggtc
    13261 gcaacggcaa ctctcagtat cgaggggccg cacgcggagc gcattctgag tgcgattggc
    13321 gctcccgtgc ctggcgaacc gatggcctcg gtagcttgga gcgcgaagct tgctgcccga
    13381 ctcaacagca ccggtatgcc gggctatttt ctgatggtac ccgctgagga aaaagatgcg
    13441 ctggtggacg aactggtgaa ggcgggcgcg gttcccgcgg atagcgagga tgcgcgtgca
    13501 gtccgtatgg agaacgggaa accgcgctat ggcgaagaca tcctggaaac ggtaattccg
    13561 caggaaaccc aggtgatgta ttccgtccat ttcaacaaag gctgctatct ggggcaggag
    13621 attgtagaac gtgtgaggtc ccggggccgc ttgaaccgca tgctggtatc gctcgacatc
    13681 gagacgcaaa ccccgccgcc cgccaataca aagataacgg tggacgagaa agaagccgga
    13741 cggatcacct ccgccgtctt ctcgcccaac cgcggcaagg tcgtcgcgtt cgcctatctt
    13801 cgcgccgaga gagtcgaagc cacgaatctg atggtggagg gcgctcccgc cggcggcgta
    13861 cgctgtccac tgaatcagcc ggagacctga cggaggcgat ccagcgcttc tttcggcgta
    13921 gcgacctgac cgcggccgac gcccagccgg acactgggtt tcacagcccc atggaaatcg
    13981 ctgcctccgg tgacggccaa gtcatacttt tccgccagac ccaggtagcg ccgtgtgtct
    14041 tcttcagtgt ggtccgagtg ccagacttca atggcgtcga ggcccgcgtc gcgaagcaag
    14101 gccacaaacg cttcctcttt ctgaacatcc cggcggccca gccggaccgg gtgagccagc
    14161 gacgaaatgc cgccgaaggt tttgatgcgc tggatggcat cgagtagcgc cggctgtatg
    14221 cgatcgacat aggctttcgc ggactcatcc aggtacaacc ggaacgcctc gtcgtaggag
    14281 gagacgtagc ccttttcgag aagaacgcgg gcgaagtgcg ggcgcccagt catggtgcgg
    14341 cccagtgcct ctacttcctc cagttttata tcgaccccga aatcccggag tctcgccgcc
    14401 aaccggcggt tgcggtctcg ccggttgctc tgcatctcga ccagccactc gcggaaatcc
    14461 gcgggagggc cgccgttgaa gaagtatgcc aacaggtgaa cgcttttgct tttggaggtt
    14521 gcctcgaagg catgcttcgt actcagctcg atgccgcaca ccaggtccag ccccacatcg
    14581 cgcgcgatca ggacggcttg gtcgtagccg gaaaacgtgt cgtgatcgga aatggcaagc
    14641 gcttccagtc ctgcctcgat cgccgcctca accagttcgc gcggagagta cgtaccgtcg
    14701 gattcgtagg tatgcgtatg gagatcgatc aatggacctg ctcgcgtgag aacgatagaa
    14761 gccccagttc ttccagttta gtccatactt tcgcggcgct ctcctccagg ctctcccgcg
    14821 aggtgtccac gacaatatcg ggctggagcg ggggctcgta aggatcggag acgccggtaa
    14881 agttcggtat ttccccagcc agcgcttttt tgtacaaacc cttcacgtct ctttccgcca
    14941 gcacttcgac gggacaatcg acgtgaactt ccaggaagtt cgcaatgcgt gtcttcaact
    15001 cctcgcgaac cgcgcggtaa ggagagatgg cggcgaccag cgcgatgact ccgttgcgcg
    15061 acaacagctc acagacaaag ccgatgcggc gaatgttcgt gtcgcggtct tccttactga
    15121 agcccaggcc ctgggaaagg ttggtccgga caacgtctcc gtccaggagc tcgacgcgcg
    15181 cgccgagttc tttcaggcgt gtgtagatgg cgtgcgatat cgtgcttttt ccggctccgg
    15241 aaagccctgt gaaccacagc gtgaatcctt tatgcatgcg cgtctgcgag aatctcccgg
    15301 agcagcacgt cgacctggcg ttcggctttg ccgtccggcg aaaagtggag gccgcattct
    15361 ttttgcgcgc cgtcttccca ccaccagcgg cc
//
LOCUS       DASTMK010000311         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_238638, whole genome shotgun sequence.
ACCESSION   DASTMK010000311 DASTMK010000000
VERSION     DASTMK010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238638"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VFQ79_13635"
     CDS             complement(<1..1485)
                     /locus_tag="VFQ79_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140758.1"
                     /translation="MRLYSAALLILLTAASGQGQQDANTRARELESKGDGWGARTLLQ
                     QAANGGEPGAARAYAEFLDRHKDPSAREAYRAAIAAADGRESRMALARRLVLLDLIAG
                     DYDSASKNLERYREYGGTELSGSVKVPPAGEPGPVAVVQIPGPIRSFARMAALSPDLL
                     PKDVLTALARNVVTNGYQASSSNEALDQTEYLKLVVRYLSQARELEKLAGAGKTIRIE
                     QCESAEAADILRVLGYRMRGACGGDVVLETVNATRAFLTIDSGFPLAELEQALRTNRP
                     FVYDYRPTEVPVQFGPEYWLSSRDKQGGEFIDVFLSDPSVCRLYLGLSKLDRETAEVL
                     RKQMPAQKIRVLSHVIDFFGGMFQIRNGKAIVPGGARSAKGWSDLVGESPDNGGAFFE
                     RLIARDDGWMASYFDALARVDGPLQAYLTEPERMKRFYSALRGRVTSPGPARPVFRSN
                     TDLMLLTTRLRVDSQGRAVIPGSIDVWKNLFINHPHGKYDGKLTR"
     gene            2191..2529
                     /locus_tag="VFQ79_13640"
     CDS             2191..2529
                     /locus_tag="VFQ79_13640"
                     /inference="COORDINATES: protein motif:HMM:NF040913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SaoD/DsrE family protein"
                     /protein_id="HEU0140759.1"
                     /translation="MKVAYIFSTQGHTVSYKLGKMILPQLEENRHGVDVVGMFFFEDN
                     CYLLQKGNPLGQRLVDVARKNGMLLMCCDQCCYEREIADKLFDGVPIGCFPDLYKALS
                     GNMPDQVITL"
     gene            2545..3405
                     /locus_tag="VFQ79_13645"
     CDS             2545..3405
                     /locus_tag="VFQ79_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0140760.1"
                     /translation="MDPKFRIAAITDEYSPDLDTALKPMVETGMKGAELRVLGGKNIM
                     DLSDAELDDAIRRIHAVGLEIISIASPILKCVLPNSPEIDSRFQHDIFASKHTFDDQP
                     RLAERAFEIAGKTGAGIIRVFSFWRTVEPDKCFDAVVNSLGELAEKASKHGVVIGLEN
                     EHACNISTAAESARVLKAVDHPNLKLVWDPANAYVSGEVPFPDGYRLLPVDRIAHVHA
                     KDCHMEGHKPIWGPLGTRSVDWKGQIKALLDDGYKGWLSLETHWQGPNNDKFQASYIS
                     GWNLRGLAAA"
BASE COUNT          746 a         1038 c         1002 g          695 t
ORIGIN      
        1 tctggtgagc ttgccatcgt acttgccgtg aggatgattg atgaagaggt tcttccacac
       61 gtcaatgctg ccggggatta cggcgcgccc ctgactgtcc acccgaagcc gtgtggtgag
      121 cagcatcagg tcggtgttgg aacggaaaac cggacgcgca ggcccggggc tggtcaccct
      181 tccacgaagt gcggagtaga agcgtttcat ccgttccggc tctgtcagat aggcctggag
      241 cggaccatcc acgcgggcaa gcgcatcgaa gtagcttgcc atccagccgt cgtcccgggc
      301 gataagtctc tcgaagaacg ctccaccgtt atcgggagac tcgccaacga gatcggacca
      361 tcctttggcg gatcgcgcgc cgcccggcac aatcgcctta ccgtttctga tctggaacat
      421 gccgccgaag aaatcgatga cgtgggaaag gacgcgaatc ttctgcgccg gcatctgctt
      481 tcgcagtacc tccgccgttt cgcggtccag cttcgacaag ccgagataga gccggcaaac
      541 ggaaggatcc gacaggaaga catcaataaa ctccccgccc tgcttgtcgc gcgaggaaag
      601 ccagtattcc ggtccgaatt gcaccggcac ctccgtagga cggtagtcgt aaacgaaagg
      661 ccggttcgtg cgcaaagcct gctcgagttc cgcgagcggg aaaccggagt caatggtcag
      721 gaacgcgcgc gtcgcattga ctgtctccag caccacgtcg ccgccgcacg cgccgcgcat
      781 acgatagccg agcactctca gaatgtcggc ggcttcggcc gactcacact gctcgatgcg
      841 gatggttttc ccggcgcccg ccaacttctc gagctcgcgc gcctgggaaa ggtagcggac
      901 caccagcttt aaatactccg tctgatccag cgcttcattg ctgctcgatg cctggtaccc
      961 attcgtgaca acattccggg ccagagcagt cagtacgtct ttgggcaaca ggtctggaga
     1021 cagcgccgcc atgcgcgcaa aggaacgtat cggccctggt atttgcacga cagccaccgg
     1081 accgggctcg ccggctgggg gcactttcac gctaccggaa agctcggtac cgccgtactc
     1141 gcgatagcgt tccaggtttt tcgaagccga gtcgtaatcg ccggcgatga gatcgagaag
     1201 gaccagccgg cgagcgagcg ccatccggga ttcgcggccg tccgcagccg ctatcgcagc
     1261 ccggtacgct tcccgcgccg acgggtcttt atgacgatcc aggaattcgg cataggcccg
     1321 agccgcgccg ggttcaccgc cgttggcagc ttgctggagc agcgtgcggg ctccccagcc
     1381 atcacccttg ctttccagtt cgcgtgccct tgtattggcg tcctgctgtc cctgtccgga
     1441 agccgccgtc aaaaggatca gaagagccgc tgagtacaat ctcattgttt tccctcgcaa
     1501 cgtctaccac ccacggcgtc actcacccgc ctgcactggt gaggaaatgt accaggtgac
     1561 aggctcctgg cggcaaacat ccgggcatcc cgtaccccgg tattcgcctt tcgtcccttc
     1621 gggtgctctg ccggtcgcct aagggctcgt ggttacttaa tgtagcactt ctgtaagatg
     1681 cccatttgga tgccaattct acggaatctg gaagagaaaa cgcaagcgcc ccgctgtaaa
     1741 cttccaaaat cggtagatct ggtactaaaa ggatggcggt acgatgatca gcgaagttcg
     1801 cactaaggga ttagaatcga agttggcccg caccgttcac gactatttta ttgaaggcta
     1861 tcgcagaacc gggcgaacca cgctgagaga cttttccggc actcctgtgc aaggttcaga
     1921 agacctgtct gccgcttttc aattgttcgt gagagaggga accgtcacac gtgtggagta
     1981 ccggtgctct tcctgtgtga cgctggtagc actgtgtgaa catgtagccc agcgcctggt
     2041 tgggcagccg gtggaaatag taagggcgct aaagccagaa ctgctggcca cgtggcatcc
     2101 cgaggttccc cggtcacacc agaatcgatc ccatctcgtg atacgggcgg tgcggtacgc
     2161 gttcgatcaa tttcaaaatc aggagccaac ttgaaagtcg cttacatctt ctccacacaa
     2221 ggacacacgg tatcgtacaa actcgggaaa atgattcttc cccagctcga ggaaaaccgg
     2281 cacggcgtcg acgttgtggg catgttcttc tttgaggaca actgttacct tttacagaaa
     2341 ggcaatccgc tcggccagcg cctggtggac gtggcacgca agaacggcat gctgttgatg
     2401 tgctgcgacc agtgctgcta cgagagagaa atcgccgaca agctgtttga cggcgtgccg
     2461 atcggatgtt tcccggatct ctataaagcg ctttccggga acatgcccga ccaggtgatt
     2521 actttatagg aacagcctga aaccatggac ccaaaattcc gaatcgcagc cattaccgac
     2581 gagtactcgc cagatctcga cacggcactg aaaccaatgg tggaaacggg catgaaaggc
     2641 gccgaactgc gcgtcctggg cggcaagaac atcatggacc tcagcgacgc cgaactggac
     2701 gatgctatac gcagaattca cgcggtagga ctggaaatca tttccatcgc ctcgcccatc
     2761 ctgaagtgcg ttctcccgaa ctcgcctgaa atcgacagcc gctttcaaca cgatattttc
     2821 gcttcgaaac acaccttcga cgaccagccc cgcctggccg agcgtgcctt cgagattgcg
     2881 ggaaagacgg gcgccggcat catccgggta ttttctttct ggcgcacggt ggaaccggat
     2941 aagtgcttcg atgcggtcgt caattctctg ggcgagttgg ccgaaaaggc gtcgaagcat
     3001 ggcgtcgtga tcggtctcga gaacgagcat gcctgcaaca tctccaccgc cgccgaatcc
     3061 gcacgggtgc tcaaagctgt cgatcatccg aacctgaagc tggtttggga tccggctaat
     3121 gcttacgttt ccggtgaagt cccgttcccg gacggctacc gcctgctgcc agtggaccgt
     3181 atcgcgcacg tacacgcgaa agactgccac atggaaggac ataagcccat ttggggccca
     3241 ttgggaacac gctcagtgga ttggaaaggc cagatcaagg cgctgctgga tgacgggtac
     3301 aaaggctggc tcagcctgga gacacactgg caggggccga ataacgataa gttccaggcg
     3361 agttacatca gcggctggaa tttacgcggt ctggccgccg cctaaaggct atcggaaccg
     3421 ggcgggctgg ccgcccgccc ggctcgcagt gttaccgctt cggaaccgtc tcacctttgc
     3481 g
//
LOCUS       DASTMK010000312         9406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_334892, whole genome shotgun sequence.
ACCESSION   DASTMK010000312 DASTMK010000000
VERSION     DASTMK010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334892"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1847)
                     /locus_tag="VFQ79_13650"
     CDS             complement(66..1847)
                     /locus_tag="VFQ79_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140761.1"
                     /translation="MTSATVLLGYQQRIDQIEALLANLQMRQGTAVAVLLLAFVTAAG
                     FCFLAFSRRAVPIWYPPLPLPAALLSLRKYSRQRLQISNFVRLRRFYVGGVERLEGRW
                     IGNGISGEEFEVPDHVYAADLNLFGRGSLFERVCTARTHLGCERLASYLQEPAGIDEI
                     RQRQAAVRELAGRTDLREKLAVLGRYDFEQSRWQTFAEWLDSPPAGFASWLRPVMLAS
                     SVVICVLSVAVMLSPSIWQAMLPIMLPIAGSNAAAGFWFQKRVRRVLKAAAPVASEIS
                     LIREGLELLASEQFVAPKLEGLKTEVASAGTVIRLLKPWFTILQERTKDWFYLPSLWL
                     LLGTQSAMAIESWRIHHRDALRRWLDAWAEFEALNSLACYAHENPKDCWAELGEGRAY
                     FSAEELGHPLIAHDRCIPNDVCLGNTYRFCVLSGSNMSGKSTLLRSIGLAAVLAFAGA
                     PVRARSLRLAVMRICASISIVDSLREGKSKFLAEVQRLREMLQLSAAGPTLFLVDEIF
                     SGTNSPDRRAAADAVVGTLAARGAIGAVSTHDIALAAIASHGGANLHMGSRASSDNPL
                     DFDYKLKRGVTTESNALAIARMAGVPI"
     gene            complement(1979..2794)
                     /locus_tag="VFQ79_13655"
     CDS             complement(1979..2794)
                     /locus_tag="VFQ79_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140762.1"
                     /translation="MQSRWDEADRLRRLQVDRARYESELARRRFLRVDPDNRLVAASL
                     EAEWNVKLRALAEAEQNYERQCQADQFKISAAQREQILALATDFPRLWNDSATANRER
                     KRMVRLLIGDITIRKGEQIKLDIRFRGGASKTLMLPLPLSFCESHRQSPAMVAEMDRL
                     LDDYNYADTARILNERGFKTGDGLPLTSIVVGYVRKAYGLKSRFDRLRQRGLLTISEM
                     ARACGVSVNTIGHWRKKGLVRGHAINDRTQFLFENPGSNPPKKHTRKVPGRAA"
     gene            3380..3961
                     /locus_tag="VFQ79_13660"
     CDS             3380..3961
                     /locus_tag="VFQ79_13660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140763.1"
                     /translation="MVWSTFFSGTGTDSIADLALLPDGRIVIGGQAGSPDLPGLTDFP
                     SGCKPWAGREQGFISVLSPGAETLLSTLVTWNPGRLLIPGNTSPHFAAAVSPKGYREY
                     ASVADQCDVCVAEPADWTFVEETTPGHLLTIFGSGLKDAPVDFNGVPAKILYTSPGQI
                     NIRTPLDLPVGQTVRYESERRTAQSNCRSGLRA"
     gene            3958..4359
                     /locus_tag="VFQ79_13665"
     CDS             3958..4359
                     /locus_tag="VFQ79_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140764.1"
                     /translation="MMPRVYMGTVPFDPANRSVPCGGDVLVGSYQPEAINENGTRNGC
                     NNRAPRSTTVELTLNGLGSARPEVTLEDLSVARIDSADYVPDYFKWKVRVRVWPAIRE
                     NGTAPFISVTPIVNGVKLPYAPLVIWTAAAP"
     gene            complement(4464..7976)
                     /locus_tag="VFQ79_13670"
     CDS             complement(4464..7976)
                     /locus_tag="VFQ79_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140765.1"
                     /translation="MKRNTFSRSAIALTCVAAGAIAILLQSPMASAQVLYGSIVGTVT
                     DQSGAVVPKAAVRVTNTSTGLTREVTTDTSGYYSIPNLLQGTYDVSIAAVGFRPLTQK
                     GVSVAINTVTRADLNLELGAVAESVTVEASAALLQTTKADVNVNLESQAVTNLPLANY
                     RNYQSLVNLVPGATPARFQNAVVDTPQRSLSTNVNGQERGANNTRVDGSANILVTLPH
                     HNVYVPPVESIEEVNIATNNFDAEQGMTGGAAVTVVTKSGTNEFHGSAFAMHANNKIR
                     AFTWDENRSGLTDKPKGIRNIVGGSLGGPIKKGKLFFFGDWEGTFERVGRSVLFSVPT
                     EDFRGGDFSRMLGSPILNASGGPIMIRTTEGGTTQLREGMIFDPYTGDVEGAGRSVFS
                     SGGRLNVIPATRVNAPMKQVLDWVPLPNRSGDLNNYFNLGTQRFNRNNADAKINWNRN
                     EKHQIWGKYSAMTALVQGDYGLGQAGGSCLCDGGVGAGDTLVQIAGIGQTYTVSPTFL
                     IDGTLGWTRFGQAVTPPDLGTNFGSEVLGLPGTNGPDPRESGLPAFYISDYSSLGNTE
                     GWNPAFRNDQSYTFNTNANWMKGAHEIRFGFDFMHHLMNHWQPELGEGPRGAFGFDPG
                     ITALNPAALEATAGFRGDTPSFENGWNGLAGFLLGTPTFSGKSSQNIKMDSLENIYAL
                     YIRDRWRATSKLTINLGLRWELYPNRTRSAGLGIESYDPTTNEVLVGGRGNIPRDNGV
                     GYSKKLFAPRIGFAYQLGKSTVVRSGYGITYHSHPWGAQALRGWYPLTIASVFEGVNG
                     FQPVMTDPNYIAAGIPNQPLGPNVGIAPLKTPDLSTGRIPLPSSDEMGYPIANRTMTR
                     GYIQSWNFIVEHKFPANFVASVGYVGTASVNGFAFLDINASQIPGSGNEGRPLYERFG
                     RTATTREWNGRTHSNYHSLQATINRRFSGGLFLKGAYTYSHAIDMAPYSDWTAFRWNA
                     ASVFHRNRATADHDVPHILQLAAVYELPFGAGKPWAATGAPGAILGGWQINTLFGAYK
                     GRPYNLTASGSTLNMPGNAQTPDLVKPDVEKLGNVGDDGTFFDTNAFARPTGVRFGNV
                     GRNTIRGPGVVNLDLSLFRKFRLTEKFNLQFRAESFNLTNTPHFANPNANANSSNFGM
                     ILATQTADALGRSREFRFGLRLGF"
     gene            8089..9183
                     /locus_tag="VFQ79_13675"
     CDS             8089..9183
                     /locus_tag="VFQ79_13675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0140766.1"
                     /translation="MRTPILRPLMALLCLVSACGAQDLASRIDEYIQAHAKVESFNGS
                     VLVARQGEVIVSKGYGLANVEHDVPNTPRTKFRLGSITKQFTSMAIAQLAVRGKLSLE
                     DTARKYVPDAQESWGKVTVRHLLTHTSGIPSFTAFPEYRQFKIEPAPPLELIARFKNK
                     PLEFEPGEKFKYNNSGYYLLGYIVEKASGQSYEAYLKQNIFDPLGMKDTGYDTNSAII
                     KGRAAGYTLRGGGWVNADYINMEVPGGAGALYSTVEDLYRWDQALYTEKLVPRQMLET
                     IFTPFKGDYAFGWVVNKRLNHRVIRHGGGIEGFNTVIARYPDERVCVIALSNLNSGAL
                     DRIGDDLAAIVFGEPYELPRARKEVSVNPE"
     gene            complement(9170..>9406)
                     /gene="rsfS"
                     /locus_tag="VFQ79_13680"
     CDS             complement(9170..>9406)
                     /gene="rsfS"
                     /locus_tag="VFQ79_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU0140767.1"
                     /translation="VICSGANARQIRAIADEVGMALKKHGERPANVEGYDNAGWVLLD
                     YGDLLVHVFSDEARSYYDLERLWRQAKEVPVTPG"
BASE COUNT         1937 a         2826 c         2699 g         1944 t
ORIGIN      
        1 agttgcccgg ttcatcttga atgtccggcg gttatggcgt cgcgaggcta tcactgctgc
       61 catcatcaaa ttggaacgcc agccatacgt gcgatcgcta atgcgttcga ttccgtagtc
      121 acgccccgtt tgagcttgta gtcgaaatct agcgggttgt ccgaactggc acggctaccc
      181 atgtgcagat ttgcgcctcc gtgggatgcg atcgcggcga gtgcgatatc gtgcgttgaa
      241 acggctccaa tcgcgccgcg ggccgcgagt gtacctacaa cggcgtccgc cgcggcgcga
      301 cggtctggcg aattggtgcc actgaagatc tcgtctacga gaaatagcgt cgggccggcg
      361 gcagagagtt gtagcatttc gcgaagtcgc tgtacttccg cgagaaactt cgattttccc
      421 tcccggaggg aatccacgat cgagatagac gcgcaaatgc gcatcaccgc gaggcgaagt
      481 gaccgcgcgc gtactggggc accagcgaat gcaaggaccg ccgcaaggcc aatagatcgc
      541 aatagcgtgc tcttgccgga catgttcgaa ccactgagaa cacagaagcg gtatgtgtta
      601 ccaagacaga cgtcgttggg gatgcaccta tcatgcgcga tgagagggtg tccaagctct
      661 tccgcgctaa agtacgccct tccttcgcct aactcagccc agcaatcctt ggggttctcg
      721 tgggcgtaac acgcaagcga gttgagggct tcaaactcgg cccaggcgtc tagccagcgg
      781 cgcagagcgt ctctgtggtg aatccgccat gattcgattg ccatagcact ttgagtgccc
      841 agcagaagcc agagggatgg caaataaaac caatccttag ttcgctcctg gaggatcgta
      901 aaccatggct tcagtagccg gatcaccgtc ccagccgagg cgacttcggt ttttaaaccc
      961 tcgagcttcg gagcgacaaa ttgctcggag gcgagcagct cgagtccttc tcgaattaag
     1021 ctgatttcgc tcgcaaccgg cgcagctgcc ttaaggactc gacggacccg tttctgaaac
     1081 cagaagcccg ctgcggcgtt tgatcccgct atcggcaaca ttatcggcag catcgcctgc
     1141 catatcgaag ggctcaacat cactgccact gaaagtacgc agatcaccac actgctagcc
     1201 aacattaccg ggcggagcca tgaggcgaac ccagctggtg gggagtccag ccactccgcg
     1261 aaagtttgcc agcgcgactg ctcaaaatcg tagcgcccca gtaccgccaa tttttcccgt
     1321 agatcggtac gcccagccaa ttcgcgaacg gcggcttggc gttggcggat ttcgtcgatg
     1381 ccggcgggct cttgtaagta agaggcaagg cgctcacacc ccagatgagt gcgcgctgtg
     1441 cacacgcgct cgaacagcga cccgcgtccg aagagattta ggtccgccgc atacacgtgg
     1501 tccgggacct cgaactcttc accggaaatc ccgttaccga tccagcggcc ctccagccgc
     1561 tccacgccgc caacgtagaa tcgccgcagt ctaacgaagt tcgagatctg taatctttgg
     1621 cgactatact ttcggagtga gaggagcgcg gctggaagag gcagaggtgg ataccaaata
     1681 ggtactgccc gccgcgagaa cgcgagaaag cagaaaccag ccgccgtcac aaatgcaagg
     1741 agcaaaacgg ccaccgcggt tccttgtcgc atctggagat tggcaagaag tgcctctatc
     1801 tgatcgattc gctgttgata tccgagcagc accgtcgcgg aggtcatagt ctaagcctat
     1861 aatggtatga ttttttacgg ccccttgccc cattctgcag agttcggtcg caaaggtgcg
     1921 aacgccacac gggagcgaag gacaccgctt tgggctgccc tgcctcaatc ggccgacatc
     1981 aggcggccct gcccggcact ttgcgagtgt gtttcttagg cgggtttgat ccaggatttt
     2041 cgaaaagaaa ctgcgttcta tcattaatgg cgtgtccgcg gaccaatcct ttcttccgcc
     2101 aatggccgat cgtgttgact gagacaccgc aagccctggc catctcagaa atggtgagca
     2161 gtccacgttg gcgaagccgg tcgaatcggc tcttcagccc gtaagccttt cggacgtagc
     2221 caaccacaat cgaagtcagc ggcaacccgt cgcccgtctt gaatcctctc tcgtttagga
     2281 ttcgagcagt atcggcgtaa ttgtaatcgt ccaagaggcg gtccatttcc gctaccatgg
     2341 cgggactctg tctgtggctc tcgcaaaagc ttaacggcag cggcaacatc aacgtcttgg
     2401 acgcgccccc gcggaaacga atgtcaagct taatctgctc gccctttcga attgtgatat
     2461 ccccaataag aagacggacc atccgcttgc gttcgcggtt ggcagtcgca gaatcgttcc
     2521 acagccgggg aaagtctgtg gcgagggcaa gaatttgctc tctttgcgct gcgctgatct
     2581 tgaactgatc cgcttggcac tgccgctcgt aattctgctc ggcctctgcc agtgcccgca
     2641 gcttcacgtt ccactccgct tccaacgacg cagcaacgag tcgattgtcg ggatcgacgc
     2701 gaagaaaccg ccgacgggcc aactcagatt catatcgggc gcggtcaact tggagtcggc
     2761 gtaatcgatc cgcttcatcc caccgcgatt gcatctcctg ctgaacgctg agggcaacct
     2821 ccagcgcaag aggagtcacc gtcgcgcaag aggagtcacc gtctccacca acatcgggcc
     2881 gacagcgaga tccaaactgt atccggatat tcgttgacag tagcccttct cgatccgatc
     2941 tacctctcgg gggcccgcgc aaatgtagtc tggagccaga cgaacgcgtg tagtgcgacg
     3001 ccgcttccgt ggaagttgtg cacaccggct tcgtagtcaa actggacgct caaggcaatc
     3061 ctgtgttgtc cgtgcgaggt gccggggctg gccccgccac ttgggataaa ggcgggaata
     3121 tctacaccgc cggcgtagac gcaaccttcg cgcctacacc aggggccttt caggtttcgc
     3181 acgaaacgcg gccgtgcgcg ggtacgtctt ttgccggact tccctgcacc taccagcaag
     3241 tcgtcaaact gagcccggaa ccttccagcc gaactaccgc gccaaccatc cgtcaccgcc
     3301 actcgcgggt ccccatcccg gattgtatcc gccaccaacg accggtttca tctcccgcct
     3361 gaacgccggc ggcagcggct tggtctggtc gacattcttc agcggaaccg ggaccgactc
     3421 catcgccgat ctcgccctcc ttcccgacgg ccgcattgtg atcggcggcc aggccggctc
     3481 gcccgatcta cccggtctta ccgattttcc ttccggctgt aaaccctggg ccggccggga
     3541 gcaagggttt atctcggtgc tcagcccggg cgcggagacg ctgctctcaa cattggtgac
     3601 gtggaaccct ggccgtttgc tgattcccgg caacacatct ccgcacttcg cagcagccgt
     3661 ttcgcccaag ggctatcgcg aatacgcttc cgttgcggac cagtgcgacg tatgcgtcgc
     3721 cgagcctgcc gactggacct ttgtcgagga aacaaccccg ggccatctgc tgacgatctt
     3781 cggtagcgga ctcaaagatg ctccggtcga tttcaacgga gttcccgcaa aaattctcta
     3841 tacgtcgccc ggacagatca acattcgtac gccgctcgat ctacccgtgg gccagacagt
     3901 ccgctacgaa tcggagcggc gcaccgcaca atcgaactgc cgttcggggt tgcgagcatg
     3961 atgccacgcg tctacatggg cacggttccg ttcgaccccg ctaaccgcag cgttccctgc
     4021 ggaggggacg tgctcgttgg ctcctatcag ccggaggcca ttaacgagaa cggcacccgc
     4081 aacggctgca acaaccgggc gccacgtagc acgacagtcg agttgacgct gaacggtctt
     4141 ggctccgctc ggccggaggt cacgcttgag gacttatcag tggcgcgtat cgacagcgcg
     4201 gattacgtcc cggactattt caagtggaag gtgcgcgtcc gcgtatggcc ggctatccgg
     4261 gagaacggca cggccccgtt catctccgtg acgcccatcg tgaacggcgt gaaactgccg
     4321 tacgcaccgc tcgtgatctg gacggcggct gctccgtaga ggcttaagaa cgcgcgccgg
     4381 caaatctaac gatacgcaca accgctccgt ggctgttgcg gctcagaaat aatggcctta
     4441 ccgagtcgcg agcgccggta aatctagaat cccaggcgca ggccgaagcg gaactctcgc
     4501 gaacggccga gcgcatctgc cgtttgcgtc gccaggatca tcccaaaatt cgagctgttc
     4561 gcattcgcat tcgggtttgc gaagtgcggc gtattggtca ggttgaatga ttccgcccgg
     4621 aactgcaggt tgaatttttc ggtcaaccgg aacttgcgga aaaggctcag gtccaggttg
     4681 acgactcccg gcccgcgtat ggtattgcgg cctacattgc caaatcggac cccggtcggc
     4741 cgcgcaaatg cgttcgtgtc aaaaaacgtg ccgtcgtcac cgacgttgcc cagtttctca
     4801 acgtccggct tcacaaggtc cggtgtttgc gcgttgcccg gcatgttcaa cgtcgatccg
     4861 gaagcggtca gattgtaagg acgtcccttg taggctccga agagagtgtt gatttgccac
     4921 ccgcccagta ttgcacccgg cgcgccagtc gcagcccagg gctttccggc gccgaagggc
     4981 agttcgtaca cagcggctag ctgaaggata tgcgggacgt cgtggtccgc cgtggcgcgg
     5041 ttgcgatgga agacgctcgc cgcattccaa cggaacgccg tccagtcgct gtaaggagcc
     5101 atgtcgatgg catgcgagta ggtgtaagcg cctttgagga acagaccgcc tgagaagcgc
     5161 cgattgatcg tcgcctgcag ggaatggtag ttgctgtgag tgcggccgtt ccactccctt
     5221 gttgtcgcgg tgcgtccgaa tcgttcgtac agtggccgcc cttcattacc cgagccggga
     5281 atctgcgaag cgttgatgtc gaggaacgcg aatccgttca ccgatgcggt cccgacatac
     5341 cccaccgacg ccacaaagtt cgcagggaat ttgtgctcga cgatgaagtt ccacgattgg
     5401 atataacccc gggtcatcgt gcggttcgcg atgggatacc ccatttcatc cgacgaaggc
     5461 agcgggattc gcccggtgct gaggtctggg gttttcagcg gcgcgatgcc gacattcgga
     5521 cccagcggtt ggttcggaat gccggcggcg atgtagttcg ggtcggtcat cacgggctgg
     5581 aaaccattca cgccctcgaa cacggaggca atcgtcagcg gataccaacc acgcaatgcc
     5641 tgggcgcccc agggatgcga gtggtatgta atgccgtagc cgctacggac caccgtagat
     5701 ttgccgagtt gatacgcgaa cccgatgcgc ggggcgaaca gtttcttgct gtagccgacg
     5761 ccattgtctc tggggatatt gccccgtcca ccgaccagca cttcattcgt cgtggggtca
     5821 tacgattcga tgccgagacc agccgatcgc gtgcggttcg ggtagagttc ccaacgcagc
     5881 cccaagttga tggtcagctt cgacgtcgca cgccagcggt cccgaatgta aagagcgtat
     5941 atgttttcga gactatccat cttaatgttc tggctgctct tccccgaaaa cgtcggtgta
     6001 cccagtagga agcccgccaa tccattccag ccattctcga aagacggagt atctccccgg
     6061 aacccggccg tggcttcaag ggcagcggga ttcagcgcag taataccggg atcaaatccg
     6121 aacgcacctc gcggaccttc tcctaattcc ggctgccagt gattcatcag gtggtgcatg
     6181 aagtcgaagc cgaaacggat ttcgtgggcg cccttcatcc agttggcgtt ggtgttgaac
     6241 gtatacgact ggtcgttgcg gaacgccggg ttccatcctt ccgtattgcc gaggctggag
     6301 tagtcggaaa tgtagaaggc aggcaatccg ctttcacgcg ggtcagggcc gtttgttcct
     6361 ggcagcccga gcacctcgct tccaaagtta gtgccaaggt cgggcggcgt gacggcctgg
     6421 ccgaaccgtg tccagccaag ggttccatcg atcaggaacg tgggtgagac cgtgtaggtt
     6481 tgtccgatgc ccgcgatctg taccagcgtg tcgccggccc cgacgccgcc atcgcacagg
     6541 cacgatccac ccgcctgccc caagccgtaa tccccctgga ccagggctgt catcgcgctg
     6601 tacttgcccc agatctggtg cttctcattt ctgttccagt tgatcttggc atcggcgttg
     6661 ttgcgattga aacgctgcgt gccgagatta aagtagttgt tcaagtcgcc agaccgatta
     6721 ggcaggggca cccaatcgag cacctgcttc atcggagcgt ttacccgtgt tgcgggaatg
     6781 acgttcagcc ggccgccgct cgagaacacg gaccgtcccg cgccttccac atccccggtg
     6841 taaggatcga aaatcattcc ctcccgaagt tgtgtcgttc cgccctcggt tgtacgaatc
     6901 atgattgggc cgccgcttgc gttcaggatg ggacttccca gcattctgct gaagtcacct
     6961 ccgcgaaaat cctccgtcgg aacggagaag agaacggagc gcccgaccct ctcgaatgtt
     7021 ccttcccagt ctccaaagaa aaacagcttg cctttcttga tcggcccgcc aaggctcccg
     7081 ccaacgatgt tccgaatacc tttgggcttg tccgtcaggc cggagcgatt ttcgtcccag
     7141 gtgaatgccc gtattttgtt gttggcatgc atggcgaaag cactgccatg aaattcattg
     7201 gtcccggact tcgtcacgac agtcaccgcg gcgccgccgg tcatgccctg ttccgcatcg
     7261 aaattgttgg tggcgatgtt tacctcttca atgctttcca ccgggggcac gtagacattg
     7321 tgatgcggca gcgtgaccag aatgttggcg gacccatcca cgcgagtgtt gttggcgccg
     7381 cgttcctggc cgttcacgtt tgtcgacaat gacctctggg gggtatcgac gacggcgttc
     7441 tgaaagcgtg ccggagtcgc tccgggaacc aggttgacga gtgactggta attgcgatag
     7501 ttcgccagag gcaggttggt aacagcttgc gattcgaggt ttacattcac atccgccttc
     7561 gttgtctgca aaagcgcagc gctggcttca accgttaccg attcggccac ggcaccgagt
     7621 tccaggttga ggtcggcacg ggtaacggta ttgatggcca cgctgacgcc tttttgtgtg
     7681 agaggtctga acccgaccgc ggcgatggaa acatcatacg taccctgaag caggttcgga
     7741 atcgagtagt aaccggacgt gtcggttgta acttcacgcg tcagtccggt tgaagtgttg
     7801 gtaacccgca ccgctgcttt gggaaccacc gcgccactct ggtcggtgac ggttcccacg
     7861 attgagccgt agagtacctg tgcggaagcc atgggcgact gcagcaagat cgcgatagcg
     7921 ccggcggcca cacaagtcag agcaattgcc gaacgggaaa atgtatttct tttcatctgc
     7981 ccttttctcc ccagaaagat gtgtaccagc agaacctact ccattcaaaa cggagtgtca
     8041 aggcgccgaa gctaggtttg tcagactctt cagtaaaata caaaagatat gaggacaccg
     8101 atccttcgcc cgctgatggc gttgctttgc ctggtctcgg cgtgtggagc ccaggatctc
     8161 gcttcgcgca tcgacgaata tatccaggct cacgcaaagg tggaatcctt caacggctcg
     8221 gtgctggtcg cgcggcaggg cgaggtgatt gtaagcaaag gctacggcct ggcaaacgtc
     8281 gaacacgacg ttcccaacac gccacggacc aagtttcgtc tgggatccat caccaaacag
     8341 ttcacgtcga tggcgattgc acaactcgct gtgcgcggca agctcagcct ggaggacacc
     8401 gcgcgcaagt atgttcccga tgcacaggaa tcgtggggca aagtcacggt gcggcatctc
     8461 ctcacgcaca cgtcagggat tccgagcttc actgcctttc cggaataccg gcagttcaag
     8521 atcgaaccgg ctccgccgct cgaactgatc gcacggttca agaataagcc gctcgaattc
     8581 gaacccggcg aaaagttcaa gtacaacaac tccggttact atttgctcgg ctacattgtt
     8641 gagaaagcgt caggacaatc ctacgaggcc tatctcaaac agaacatctt cgatccgctg
     8701 gggatgaaag acaccggcta cgacacaaac agcgccatca ttaaagggcg cgcggccggc
     8761 tacacgcttc gcggcggcgg ttgggtgaac gccgactaca tcaacatgga ggttcccggc
     8821 ggggcgggag cgttgtattc cacagtcgaa gatctctacc ggtgggacca ggcgctgtat
     8881 acggaaaaac ttgtgccccg gcagatgctg gagacgattt tcacgccatt caaaggagac
     8941 tacgctttcg gctgggtggt gaacaaacgg ctgaaccacc gcgtgatccg ccacggcggc
     9001 ggcatcgaag gctttaacac ggtcatcgcc cgttacccgg atgagcgtgt ctgcgtcatc
     9061 gcgctcagca atctaaacag cggcgcactc gatcgaatcg gcgacgatct cgccgcgatt
     9121 gtttttggcg aaccatacga gttgccgcgc gctcgaaagg aagtcagcgt caacccggag
     9181 tgacgggcac ctctttggct tgccgccaga gccgctccag atcgtaatag cttcgtgctt
     9241 cgtccgagaa gacgtgcacc agcagatcgc cataatccag aagcacccag ccggcgttat
     9301 cgtagccttc cacgttcgca ggccgttcgc cgtgtttctt cagcgccatc ccgacttcgt
     9361 cggcgatggc ccggatctgc cgcgcgttcg cacccgagca gatcac
//
LOCUS       DASTMK010000313         7643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_367183, whole genome shotgun sequence.
ACCESSION   DASTMK010000313 DASTMK010000000
VERSION     DASTMK010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367183"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..356
                     /locus_tag="VFQ79_13685"
     CDS             204..356
                     /locus_tag="VFQ79_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140768.1"
                     /translation="MAIAEMDDLWVYDNSPVGGPPRLVLESEKGIVRFLVDDPPAWLV
                     TALDLF"
     gene            complement(433..4137)
                     /gene="smc"
                     /locus_tag="VFQ79_13690"
     CDS             complement(433..4137)
                     /gene="smc"
                     /locus_tag="VFQ79_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU0140769.1"
                     /translation="MLKLKRIEIQGFKSFFDRTEMRFNGAGIAAVVGPNGCGKSNLSD
                     AISWVLGEQSAKSLRGARMEDVIFAGTRDRKPLSMASVTMTLLDPSFYLDAAVHIDKA
                     NGHANGKNGTSAPTQPVNGQQQKPHEITITRRLFRSGESEYLIDGRQARLRDIQDIFM
                     GTGLGPESYAIIEQGRIGQILSSKPQDRRAVIEEAAGISRFKTKRRLAEAKLEGAKQN
                     LTRVFDILEEVGRQVNSLKRQAAKAKRYEELRADMMAQLRCTLIGRFRTLEREAARTA
                     IELNLAAADFQTLHTDVAAREQEQSRLQGQCFATDQELTDARKHLAELQLEAERIRSR
                     MERQAAQIADFEQRMGQGESESEQLDQRIAGMEKELSAHAEALAELEVRTEEARGKLT
                     AKNDEREKLNSVLRERERQMEGARQQVLRLLGEASMLKNQLAQIDGFLASMDRESARV
                     RREEEDAAADIETLGGLKADLAEKLGDRETELALLSEQRRVTEHDLSTHRNKAVETRR
                     TLEQARGELSRITARKDSLEDVLAHRTYTTESVRRLFTVIEKGQAGDLRPLGVLADFV
                     EVDAAWEKAAEEFLHEELEYVVVKDWDDAAKGLDLLRANFEGRATFLVRPEPDAAAGP
                     GQSSPTGVTAPLRDVLRLTGAMAHASAGLLPRLVQCFLVEDRGTAQRLASEHPSLYFL
                     LPDGACYHGYALTGGRKSGSGPLALKRELRDLRAQIQSRQKEFDDTQALLASLENEIS
                     DLNLELERVRSTQEVREKEKVALDHEMRKLSDELTRAESRLSVAQMELKRLENERTRA
                     AEQKQRNQVLVEEKEIERAQQEQILEQGREDLSEFQSQVTRVSEEHSQLRVQVAEIEE
                     RRRSERAAQARLEAQHREMAARRRQIATEMERLGAERARLLADNIDLDSKAAALAENI
                     QLGDVAVRELETREQTERTALVDLEEALKQLRVRMQEAQEKRSQIELELVKLQAELKY
                     LDETSRKELNAPLEELAAGEEVVPDDDVLAEAEEKYRELKSRIDALGPVNPQALEEFQ
                     EAQQRYDFLNAQRQDLLESIRDTEKAIHEIDVETRKRFTDAFEAINGYFKELFQTLFG
                     GGTGEMRLTDETNASESGIDLVASPPGKKLQNVLLLSGGEKALTAIALLMAIFRYQPS
                     PFCILDEVDAPLDEPNIERLTRLLKEMSAQTQFIVITHAKRTMEAAQSLYGVTMQEPG
                     ISKLVSVKFQAQPAPPPPPVETEASPFMTQ"
     gene            4437..4525
                     /locus_tag="VFQ79_13695"
     tRNA            4437..4525
                     /locus_tag="VFQ79_13695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4471..4473,aa:Leu,seq:taa)
     gene            4624..5697
                     /gene="bchI"
                     /locus_tag="VFQ79_13700"
     CDS             4624..5697
                     /gene="bchI"
                     /locus_tag="VFQ79_13700"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0010007 - magnesium chelatase complex
                     [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HEU0140770.1"
                     /translation="MPFADPSSASIYPFAAIVGQEEMQLALILNVIEPLTGGALILGH
                     RGTGKSTAVRALADLLPPIKKIKGCLYGCDPAWPAEWCSDCRGRSRRRVPEEQGRVPV
                     VELPLGATEDRVCGTLDIERALTAGIKTFEPGLLAWANRGFLYIDEVNLLEDHIVDLL
                     LDVAASGRNIVEREGISVSHPARFVLVGSGNPEEGELRPQLLDRFGLHARILTITDVA
                     ARVEIVRRRERFERDPVRFRAEMDRHQRSLRRRIVNAKRLLPEITVHDDLLYKIGELC
                     VRLRIDGHRGELTILRAGKALAAFEGRMRVTAADVRTVAPMALCHRLRRDAFEEIDPG
                     FRVTRALDEIIPEDKSQTRAVAG"
     gene            5694..7502
                     /locus_tag="VFQ79_13705"
     CDS             5694..7502
                     /locus_tag="VFQ79_13705"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0140771.1"
                     /translation="MRTIPNHPEALAFPLPAIVGMNRVTRVLLLLAVDPGLKGIVIQG
                     PGGIAKSVMACAFRSVVSGAAGEPPFVHITPGVTKDQLLGGLDIEATLRTGRRQVEPG
                     LLAQVHGGFLVADDLNLFDAGAASCIRSALTDHKVAEFSLIATCNPEGGELRRSLADS
                     VALHVTQTKRLPPRDRVELLHRIAAFDRDPFEFSLRYTPALELLRERVAGARRRLPDV
                     QMTLSDYSELCAATLELGIEGHLAELFAARLARAHAAWNGRLRVERDDLEAAMRLVLE
                     PRAAGRQSGTSSPEAEHSPEETLCGEPGRGAEDRIFPTVPANPPDGVMDWPSSMAAKN
                     RKGTARRLSKGGDGISWTQGRYVRPLSRKPSGGGRLAVEATLRAAAPLQILRSKSDGA
                     APVIRIAKDDLRFKEFREKQGLLIIFAVDASGSMALQRISQAKGALIRLLHGAYLHRD
                     QVALIAFRGETAEVLLPPGRSVELAKRAIDVIPTGGATPLAAALESVLQLARRSAARG
                     VRQTLLVLLTDGHANIGSQQLQAMAAALRDEGIASVVIDAGNRFIGNSKAGHVAQMLG
                     GRHVFLPHWENGAVADAVSGAAESLRRQIGGTHATG"
BASE COUNT         1387 a         2387 c         2262 g         1607 t
ORIGIN      
        1 aagagggtag gtctacacta catcttgatt ttcagaagtg gtgattgcaa acgcaggact
       61 tgctcccgtc caaagcctca aaatcgttaa aaccgacggt cccgtgacga acttgggtta
      121 aagcccgcgc ggacgccggc ggccatagcg cttcagagac gacccttcgg gctacctacg
      181 cagccagcct ggccaatctt ccgatggcca ttgccgagat ggatgatctc tgggtttatg
      241 acaactcacc tgtcggtgga ccgccaaggt tggttttgga aagcgagaag ggcatcgtcc
      301 gttttctggt ggacgacccg ccagcctggc tcgtgaccgc gctcgacctt ttctgaagag
      361 tcggtcccga gccgctttcg ctgcgcgcgt gatgggcggg ggaggtggag ccgcgcgtgc
      421 cagcggcggc aatcactgcg tcatgaacgg cgaagcctcc gtttccaccg gcgggggagg
      481 tggcgccggc tgggcctgga actttaccga gacgagtttt gaaataccgg gttcctgcat
      541 ggtcaccccg tataagctct gcgccgcttc catcgtgcgt ttcgcgtgcg tgatgacgat
      601 gaactgcgtc tgcgcggaca tctccttcaa caggcgtgtc agtctctcga tgttcggttc
      661 gtcgagtggc gcatccactt catccaggat gcagaacgga ctcggctgat aacggaagat
      721 cgccatcagc agtgcaatgg ccgtgagtgc cttttccccg cccgaaagca acagcacgtt
      781 ctgtagcttc ttgcccggag gcgacgccac caggtctatg cccgattccg aagcgttcgt
      841 ttcgtccgtc agcctcattt cgccggtgcc gccgccgaac agcgtctgga acaactcctt
      901 gaagtaaccg ttgatcgctt caaaggcatc cgtaaaccgc ttccgcgttt cgacgtcgat
      961 ctcgtggatt gccttctccg tatcgcgaat cgattcgagc agatcctgcc gctgggcatt
     1021 gaggaaatcg tagcgctgct gggcttcctg gaactcttcg agagcctgcg ggttcaccgg
     1081 accgagcgcg tcgattcgcg acttcagttc gcgatacttc tcctccgcct cggccagcac
     1141 atcgtcatcc ggcaccactt cttcgccggc ggcaagttct tccaggggag cgttcaactc
     1201 tttccggctc gtttcgtcca ggtacttgag ctccgcctga agtttcacaa gctctaactc
     1261 gatttgagac cgcttctcct gcgcttcctg catccggacc cgcagttgct tcaatgcctc
     1321 ttcgaggtca accagggccg tcctctccgt ctgctcgcgt gtctccaact cgcggactgc
     1381 gacgtcgccg agctgtatgt tttccgccag cgccgcggcc ttcgaatcga gatcgatgtt
     1441 gtcggccagc agccgtgcgc gctcggcccc cagccgctcc atttcagtgg cgatctgccg
     1501 ccggcgggca gccatctcgc gatgttgcgc ttcgagccgt gcctgcgctg cgcgttccga
     1561 ccgtcggcgc tcttcgattt ccgccacctg cacacggagc tgcgaatgct cctcggaaac
     1621 gcgagtgact tgcgactgaa actcggaaag atcctcgcgg ccctgctcca gaatctgttc
     1681 ctgctgtgcc cgctcgattt ccttctcttc gaccagaacc tggttccgct gtttctgttc
     1741 ggccgcacgg gttctttcgt tctccagccg cttcaattcc atctgggcga cagagagccg
     1801 cgattcggcg cgcgtcagct catcggaaag ctttcgcatt tcgtgatcga gcgcgacctt
     1861 ctctttctca cgcacctctt gcgtactgcg aacccgctcc aactccaggt tgagatccga
     1921 gatctcgttc tccagagagg caagcagcgc ctgagtatcg tcgaattcct tttgccgcga
     1981 ttggatttgg gctctcaagt cgcgcaattc acgcttgagt gcgagcggtc cgctgccgct
     2041 ctttcttccg cctgtaagag catatccgtg atagcaggcg ccgtccggca gcaggaagta
     2101 gagcgagggg tgttcggatg cgagcctttg cgcggtaccg cggtcttcca ccaggaagca
     2161 ctgcacaagc cttggtaata gaccggcgga cgcgtgtgcc attgcgcccg tcaggcgcaa
     2221 gacgtccctg agcggcgccg tgaccccagt cggcgaactc tgcccgggac ccgcggcggc
     2281 gtccggctcc gggcggacca ggaacgtagc gcgtccctcg aaattcgccc gcagcagatc
     2341 gagccctttg gccgcatcgt cccagtcttt tacgaccaca tattcgagtt cttcatgcag
     2401 aaactcttcg gccgccttct cccaggcagc gtctacttcg acaaaatccg ccagcactcc
     2461 gagaggccga agatcgcccg cctgtccctt ctcgatgacc gtgaacagcc gtctgacgga
     2521 ttccgtcgta taagtgcggt gcgccagaac atcttcgagc gaatcctttc gcgccgtgat
     2581 gcgcgacagt tcgccgcgag cctgttcaag cgtgcgccgc gtctccaccg ctttattgcg
     2641 gtgggtcgag aggtcgtgtt ctgtcacgcg gcgctgttca gacagcagag ccagctcggt
     2701 ctcgcggtcg cccagctttt ccgccaaatc cgccttgagc ccgcccaatg tctcgatgtc
     2761 cgcagccgca tcttcttcct cccggcggac gcgcgcggat tcgcgatcca tcgatgccag
     2821 gaagccgtca atctgcgcga gttgattttt gagcatggaa gcttcgccca acagccgcaa
     2881 cacctgctgc cgtgctcctt ccatttgacg ctcccgttcg cggagaacgg agttcagctt
     2941 ctcgcgctcg tcgttcttgg ccgtcagttt tccacgagcc tcttccgtcc ggacttccag
     3001 ttccgcgaga gcttcggcat gcgccgacag ctctttttcc attccggcga tccgctgatc
     3061 cagctgttcg gattcggatt ccccctggcc cattctctgt tcgaaatcgg cgatctgcgc
     3121 tgcctggcgc tccatccggc tacggatacg ctccgcttcg agctgaagtt cggcaaggtg
     3181 cttgcgcgca tcggtcaatt cctgatcggt cgcgaaacat tgtccctgaa gacggctctg
     3241 ttcctgctcc ctggccgcga catcggtatg gagcgtctgg aaatccgcgg ctgccagatt
     3301 caactcgatt gcggtcctgg cggcttcgcg ctcgagcgtg cgaaaacggc cgatcagcgt
     3361 acaccggagt tgcgccatca tgtcggcacg caactcctca taccgttttg ctttggccgc
     3421 ctggcgcttc agggagttca cctggcgtcc gacctcctcc aggatgtcaa acacccgcgt
     3481 caggttctgt ttcgcgcctt cgagcttcgc ttcggccagc cggcgcttcg ttttgaaccg
     3541 gctgatgccg gcggcttctt cgatcaccgc acgccggtcc tggggtttcg aactcaggat
     3601 ttggccgatg cgcccctgct caataatggc gtacgactcc ggaccaagcc cggtccccat
     3661 aaagatgtcc tggatatcgc gcagccgggc ctgccgcccg tcaatcagat attcactctc
     3721 gcctgagcga aacagtcgcc gggtaatcgt gatttcgtgc ggcttctgct gctggccgtt
     3781 gacgggctgc gtcggtgcgg acgtgccgtt ctttccgttc gcgtggccgt ttgccttgtc
     3841 tatatgaacc gccgcgtcga gatagaacga ggggtccagg agcgtcatcg taaccgacgc
     3901 catactcaac ggcttgcggt cgcgcgtgcc ggcaaagata acatcttcca tccgcgcgcc
     3961 gcgcagactc ttcgcggact gttcccccag cacccaactg atcgcgtcgc tcagattcga
     4021 cttgccgcag ccgttcggtc cgaccacggc ggcgatgcca gccccgttaa atcgcatctc
     4081 cgtgcggtcg aagaacgact taaacccctg tatctctatg cgtttgagtt ttaacaattc
     4141 atgtacctct taaagcatga actcgcgaga gcacagatac tctcaactcg ggggatgact
     4201 ccatagtaag gagccgatac cgaaatgtaa aggacatgcc gtaccaaatt ctggggtcat
     4261 cctaaaattg acgcaacata ttgtggtctg cttgccaagc aggtccgaaa agacaaagca
     4321 gcccgctgtt agatgatttg ataaaggaat cgctcgcagt gattaccgct tggaggaaag
     4381 ccagagcacc ttcattataa acgctcagcg ccttacatgc tacgctgaat gcaaaggccc
     4441 gggtggcgaa acaggcagac gcaagggact taaaatccct tgtcccgtaa gggacgtgcg
     4501 ggttcgaccc ccgccccggg caccactcca ttccaccagc acccattcgc atcctcaact
     4561 ttaaggcttg cggccagccg gttccgcact acaatagcag caatacttca gaggaggcca
     4621 tggatgccct tcgccgatcc cagttctgcg tccatctatc ccttcgcggc gatcgtgggc
     4681 caggaagaga tgcaactggc cctgatcctg aacgtaattg aaccgttgac gggtggggct
     4741 ctgatactgg gccaccgcgg tacaggcaag tccacggccg ttcgcgccct ggcggatctg
     4801 ctgccaccga ttaagaaaat caaaggctgc ctctacggat gcgacccggc gtggccggcg
     4861 gaatggtgct cggactgccg gggccgctcc cgcagacggg tgcctgagga gcaagggcgt
     4921 gtgccggtcg ttgaattgcc gctgggcgct acggaagatc gggtgtgcgg aacgctggac
     4981 atcgaacggg cgctcaccgc gggcatcaag acctttgaac cgggtctgct ggcgtgggcc
     5041 aaccggggtt tcctgtacat tgatgaagtc aacctcctgg aggatcacat tgtggatctg
     5101 ctcctggatg tcgctgccag cggacgcaat attgtcgagc gggaggggat cagtgtcagc
     5161 cacccggcgc gattcgtact ggtcggctcc ggcaacccgg aagagggcga actgcggcct
     5221 cagcttctgg accggttcgg gttgcacgcc agaatcctca cgattaccga tgtcgccgcc
     5281 cgcgtggaga tcgttcggcg aagggaacgg ttcgagcgtg atcctgtccg gttccgcgcc
     5341 gaaatggacc gacatcaacg ttcgctccgg cgccgcatcg ttaacgccaa gcggctgctc
     5401 cccgaaataa cggtgcacga cgatctgctc tacaagatcg gggagctttg cgtccgcctg
     5461 cgaattgacg gccaccgcgg tgagctgacc atcctacgcg cgggcaaggc gctcgctgca
     5521 ttcgaagggc gcatgcgggt taccgcggcc gatgtccgga cagtcgcccc gatggctctg
     5581 tgccaccggt tgcgaaggga tgcctttgag gagatcgacc cggggttccg cgtcacgcgg
     5641 gcactggatg aaatcattcc cgaagacaag tcacaaacca gagcggttgc cggatgagga
     5701 cgattcccaa tcaccccgag gctcttgctt ttcccttgcc ggccattgtc ggaatgaacc
     5761 gggttacgcg agtactgttg ttgttggcgg tagatcccgg cctgaaaggc atcgtcatcc
     5821 aggggccggg cggtatagcc aaaagtgtca tggcgtgcgc tttccggtct gttgtatctg
     5881 gcgcagccgg ggaaccgcct ttcgtacaca taacccctgg cgtcaccaaa gatcaattgc
     5941 tcgggggctt ggacatcgag gccacgctcc ggaccggcag acggcaggtc gagccgggct
     6001 tgctcgccca ggtacatggc ggctttctgg tagcggatga tctcaacctc ttcgatgcgg
     6061 gagcggcgtc atgcatacgg agcgcactta ccgatcacaa ggtagcggag ttttcgctga
     6121 tcgcgacctg taacccggaa ggaggcgaac tgcgccgttc gctagccgat tcggtggccc
     6181 tccacgtcac acaaacgaaa cgcctgccac cccgtgacag ggtggaacta cttcaccgca
     6241 tcgcggcatt cgatcgggat ccgtttgagt tctcgctccg ctacacaccg gcgttggagt
     6301 tgctcagaga acgtgttgcc ggtgcacgac gccgtttgcc ggatgtgcaa atgacgcttt
     6361 cggactacag cgaattgtgc gcggcaacgc tcgagctagg gatcgaaggc cacctggccg
     6421 aactgtttgc cgcgcggctt gcccgcgcac atgcggcctg gaacggacgt ctccgtgtgg
     6481 agagggacga tctcgaagcg gcgatgcgtc tggttctgga gcctcgcgcc gcggggcggc
     6541 aatccgggac ctccagtcct gaggcggagc attcgccgga ggaaacactt tgtggagaac
     6601 ctggccgggg cgccgaagat cggattttcc ccaccgttcc cgcgaatccg cccgatggcg
     6661 tcatggactg gccttcgtcg atggctgcca agaatcgcaa aggaacggcg cgcaggctga
     6721 gcaaaggcgg cgacggcatc agttggaccc aaggccggta tgtgcgtccg ctttcccgaa
     6781 agccctccgg cggcggacgg ctcgcggttg aagccacctt gcgcgcggcg gctccgctcc
     6841 agatcctgcg ctcgaaatcc gatggcgccg cgccagttat ccgaatcgca aaggacgatc
     6901 tccggttcaa agagttccgg gaaaaacagg gcctgctgat catttttgct gtggatgcga
     6961 gcggcagcat ggcgcttcaa cgcatcagcc aagccaaggg agccctgatt cgcttacttc
     7021 acggggcgta cctgcaccgg gaccaggtgg cgctgatcgc gttccggggc gagacggccg
     7081 aagttctcct tccgcccggc cggagcgtag agttggccaa acgcgccatc gatgtgatac
     7141 cgaccggcgg tgcgacgccg ttggccgccg cacttgaaag cgtgctccaa ctggctcgcc
     7201 ggagcgcagc ccgtggtgtt cgccaaacac ttttggtatt gctgacggac ggtcacgcca
     7261 atatcggttc gcaacaactc caggcgatgg ccgcggcgtt gcgagacgag ggcattgcgt
     7321 ccgtcgtgat agatgccgga aatcgcttta tcggaaattc gaaggcggga cacgttgcac
     7381 aaatgctcgg cgggcggcat gtctttcttc cccattggga gaatggcgcg gtggcggatg
     7441 cggtctctgg tgcggccgaa agcttgagga ggcaaattgg cggaacccac gcgaccggct
     7501 gaagagcggg accggatcgt cctacgtctc tatgagcagt tgctcgaaat cgagcagcgg
     7561 ctgattccca ccggaatgca cgtattcggc aagcctggcg cgcaaaccga acggggagac
     7621 atgctccgaa tggtcgcgtc gtt
//
LOCUS       DASTMK010000314         9635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_16919, whole genome shotgun sequence.
ACCESSION   DASTMK010000314 DASTMK010000000
VERSION     DASTMK010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16919"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..741)
                     /gene="trmD"
                     /locus_tag="VFQ79_13710"
     CDS             complement(19..741)
                     /gene="trmD"
                     /locus_tag="VFQ79_13710"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU0140772.1"
                     /translation="MIFHVLTIFPGFFQGPFEHGVVAKAIQAGILDLQVHDLRNWAKD
                     RHKTVDDRPFGGGEGMLLKAEPIFEAVESILAGGIPGGARRGHVVLLSAQGRLFSQEI
                     ARRLATLDELLLICGRYEGVDERVAQYLADDEISIGDYVLSGGELPAAVVVDAVARLL
                     PGVLGNEASTVQESFSAGEGILDCPQYTRPAEYRGWRVPDVLLGGHHAEIRKWRHQAA
                     LEKTARLRSDLLKSFEKECDDE"
     gene            complement(738..1301)
                     /gene="rimM"
                     /locus_tag="VFQ79_13715"
     CDS             complement(738..1301)
                     /gene="rimM"
                     /locus_tag="VFQ79_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU0140773.1"
                     /translation="MGLHSGPWNPDDWVTVAQVIRPWGNRGEVAVISLTSQADRFETL
                     REVYLFGEDGPLGEDQPRRIESVWEHRGRLIIKFRGIDTITDAEPLRGAEMKIPLAER
                     PELPEGEYYHSDLAGCEVIDRNGNRIGRVRNWLDEGGSGVLSVEPAGNGEDVLIPFAT
                     SICKEIDVKARRIVVDLPEGLLEINRR"
     gene            complement(1306..1545)
                     /locus_tag="VFQ79_13720"
     CDS             complement(1306..1545)
                     /locus_tag="VFQ79_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU0140774.1"
                     /translation="MTGMKELVEDIAKALVDIPEEVSVREVQGEQVTVLELRVAPSDL
                     GKVIGKQGRTARSIRTLLGAAGMKLNRRFTLEILE"
     gene            complement(1677..1934)
                     /gene="rpsP"
                     /locus_tag="VFQ79_13725"
     CDS             complement(1677..1934)
                     /gene="rpsP"
                     /locus_tag="VFQ79_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU0140775.1"
                     /translation="MLMIRLARFGAKKKPSYRVVVIEKDRARNSRSVEVVGYYNPVTQ
                     PATVHFDHERIEHWLKNGAKPSDTVARLLKANPAPAAELVA"
     gene            complement(1990..3339)
                     /gene="ffh"
                     /locus_tag="VFQ79_13730"
     CDS             complement(1990..3339)
                     /gene="ffh"
                     /locus_tag="VFQ79_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU0140776.1"
                     /translation="MFENLSEKLQRVFKNLRGEGKLTAENMEEALREIRVALLEADVH
                     FRVVKQLIANVKEKAMGEEVLTALSPAQQVVKIVRDELIKILGTHQSKLRFANEPPTA
                     ILIVGLQGSGKTTSTGKLARHLSKTGHSPLMVSVDVYRPAARHQLSVLGKDLKLPVYA
                     GAPEEKDPLELARSARREAVQTGRDLVLVDTAGRLHIDDQLMEELQSLKEHLNPTEIL
                     FVADAMTGQDAVKSADEFHKRLGITGVILTKMDGDARGGAALSIRTVTGQPLKYIGTG
                     EKMDAIEPFHPDRTASRILGMGDVLSFIEKVEENIDQKQAEEMQRKLIENEFTLDDFR
                     NQIKQLKKLGPLESILGMMPQVGILKDLKNVKVDENEISRIVAIIDSMTPHERANHMV
                     VNGSRRRRIAKGSGTSVQDVNNLLKQYAQARKMMKTFSGGFMGKKLGKLKLPFLEQQ"
     gene            complement(3486..5444)
                     /locus_tag="VFQ79_13735"
     CDS             complement(3486..5444)
                     /locus_tag="VFQ79_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0140777.1"
                     /translation="MTRACFVGLVALMASVAPAQDTRPGIDVRHYKIDAEINPNTQSI
                     KAIVQVQFVAVDQGLSSAVFELNNAMNVSQVVDENGSPVPASRNQQDFTIRVTLPTAL
                     EKGKPSSLTFTYDGDFNGREDASPVYGIKFATIQNDFAYLMYPARWFPVNNYTADRYT
                     ADMRITVPTPFKVVAGGAETAEHTSPDRVRYQVSFTQPSFPGSIAVVNGEPSTVSSQG
                     VTTTLYFRTRKDMASAYGDEIGKAMTQLTSLFGLPPFANLTVVETELGAPQGYSAPGI
                     LFFSPRGIGQQVNARLIANQTARQWWGGLLSPVNRNHIWLSNGFARYSEALYLEHLNG
                     QPALESEMRDVYIEALTVQNLPVSQSSRLEDYSPEFWATTAAKGAAVLNMLRYVIGDE
                     NFTRLIKEFPSEHAWKEVSTADFRTAAEKISGQNLDYFFIQWIESSGAPEFSMEYTIF
                     RTQKGFRVMGKIAQDLDTFRMPVELRIETEGNPEEKRVEVSGTSSEFVVETFGKPKRV
                     QIDPHNRILRFNDDVRVAVAIRRGEQFAEIGEFGEALKEYQRALDVNRNSSLAHYRVA
                     EVFFLQNNYQSAANEFREALNGDLEPKWTEAWAHLNLGKIFDVTGQRDRAVNEYTLAA
                     RTKDNTQGAQEEVQKYLKEPYQRERKNF"
     gene            complement(5441..6595)
                     /gene="lpxB"
                     /locus_tag="VFQ79_13740"
     CDS             complement(5441..6595)
                     /gene="lpxB"
                     /locus_tag="VFQ79_13740"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU0140778.1"
                     /translation="MSLRIFVSAGEASGDSYAASLVRTLRSRLPGAEFYGCAGGRMQA
                     AGVQAILDAGELSVVGLVEVIRHLPRIYGRFRELMSYARRHRPDIAILTDSPDFNLPV
                     ARRLHREGVPVVYLVAPQVWAWRRGRLSQLRRNVDRLLCIFPFEEQFFKEHNVAATYI
                     GHPLAGRVMPSLEKGEFFRKHRIPLDRPVIAILPGSRPGEISRHLPPVLEAVNRLYGR
                     RAVSFLLAAPAGFSQKFGSSFYADRIGRAPVKIIEGETWDVVGHSDLALAASGTIATE
                     TALLGVPMITFYKVNHSSWVLGKFLVRVPFYTMVNLVAGRAVVPELMQSELTGERLAQ
                     EAERLLDDESARCEMRLGLRKVATRLRTDGDPMERAADIVQELLQRKFTT"
     gene            6656..7426
                     /locus_tag="VFQ79_13745"
     CDS             6656..7426
                     /locus_tag="VFQ79_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140779.1"
                     /translation="MSSQTDLARLAVEDFVERFRGEVVPLSNRFSYFFVALPLSEEDT
                     REYLQEPISALPPKLLPVLPRISIFLVPYLERANGREKGHGKPEYVVCLDKPAEQRHT
                     VMSKITTEDETTFVFAIKDQEVADYHYHLYRALAEAAAERWSIEVQNQFFSLLREELG
                     ANVHGEVDESSWHEKQALLRRQTNVRRETKVFRSYAKQAFVDTLTLYLHGICCDIDVD
                     TGPRQLPSRYLRRRLQLMQTFFPPPSGYAVFPEDLKPE"
     gene            complement(7445..>9635)
                     /locus_tag="VFQ79_13750"
     CDS             complement(7445..>9635)
                     /locus_tag="VFQ79_13750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0140780.1"
                     /translation="EAVSPVLSQVLAARGIEKLYSHQADAFNAVESGRHVVIVTPTAS
                     GKTLCYNLPVLNSLLSDPGIRALYLFPTKALAEDQLSELKATIDASGAGIGAHTYDGD
                     TPQDARKAIRERANIILTNPDMLHSGILPHHTRWAKCFERLRYVVIDELHYYRGIYGS
                     HLANILRRLKRICEFYGSSPQFICCSATIANPAELAEALTESRVECIDSNGAPGGDKY
                     FIFYNPPMVNHQLGIRRSYLGESRRIAGELISHGHQTLVFANNRLATEVLLTYLKHDC
                     ERRTYPPASIRGYRGGYLPRERREIERELREGRIRGVVATNALELGIDIGSLDCVVMA
                     GYPGTIASTWQRAGRAGRRNTASVALLVASSNSLDQFIVEHPDYFFGQSPEQAYINPD
                     NLEILLNHLKCAAFELPIRDGEKFGPHATGELCRFLAETGVLHHSGECWHWTSDTYPA
                     DAISLRAVTSDNFVVVDTTGEPQVIAEVSFPTALTSLHEKAIYLHEARQYEVERFDYE
                     NRKAYVRRVECEYYTDAIDYTQVKILEQVECSPLAPATAAHGDVRVNRQIVGFKKIKF
                     HTNENVGAGNLSMPEQEMHTTSFWLHFPASFFAPFEGVSPTERQEGLAGAANVLRSVA
                     GLLLMCDPRDLGVAVTEDNSPGTVSFEPDLHLYDNFPGGIGQSVPLLRMARTLVSSAR
                     QVVEACPCEAGCPSCVGPAKEAGSRGKEIALQILKALSGDQPTDAVA"
BASE COUNT         1970 a         3020 c         2585 g         2060 t
ORIGIN      
        1 actttcgtta acagcgcgtt attcatcgtc gcattccttc tcgaaactct tcaataagtc
       61 ggaccggagc cgggcagtct tttcgagcgc cgcctgatgc cgccatttgc ggatttccgc
      121 atgatgtccg ccgagcaaaa cgtccggcac ccgccaaccg cggtactccg ccggacgcgt
      181 gtactgcggg caatccagaa ttccttctcc ggcactgaac gattcctgca ccgtcgaagc
      241 ctcatttccc agaacaccag gcaacaggcg ggctactgcg tccaccacca ccgcggcggg
      301 cagctcaccc ccgctcagca cataatcccc aatcgaaatc tcgtcgtcag ccagatactg
      361 cgcaacacgc tcgtccacgc cttcatagcg tccgcaaatc aaaagcagct catcgagcgt
      421 ggcgagccgg cgtgcaatct cctgcgagaa cagccggcct tgagcggaaa gcagaactac
      481 gtgccccctc ctggcgcctc ccggtatccc accggccaaa atcgattcta ccgcctcgaa
      541 gatgggctcc gctttgagca gcataccctc tcccccaccg aacggccggt catccacggt
      601 cttatgccgg tctttcgccc agttcctcaa gtcatgaact tggaggtcga ggattccggc
      661 ttggatcgct ttcgctacaa cgccatgctc gaacggcccc tggaaaaacc cagggaaaat
      721 cgtcagaaca tgaaatatca tcggcggttg atctctaaga ggccctccgg caggtccact
      781 acgatacgtc tggctttcac gtcaatctct ttgcaaatcg aagttgcgaa cggaatgagt
      841 acgtcctctc cgttcccggc cggctcaacg ctcaataccc cggagccgcc ttcatccaac
      901 caattcctga cccgcccaat ccggttgccg ttccggtcaa tcacttcgca gccggcaaga
      961 tcggagtgat agtattctcc ttccggcaac tccggccgct cagccagcgg aatcttcatc
     1021 tcggcgcccc gcagcggctc agcgtccgta atcgtgtcga tcccgcgaaa cttaataatc
     1081 agccgtcccc ggtgttccca gaccgattca atacgcctgg gctggtcctc accgagaggt
     1141 ccgtcctcac caaaaagata aacctcccgg agcgtttcaa accggtcggc ctggctggtc
     1201 agcgatatca ccgccacctc gccccgattt ccccacggac gaatcacctg tgcgactgtg
     1261 acccaatcgt ccgggttcca tggaccggaa tgcagcccca gaggattact ccagaatctc
     1321 caatgtaaag cgccggttca gcttcatgcc ggcagcgcca agcagagtgc ggatggaacg
     1381 ggcggttctc ccctgctttc caatcacttt gcccaaatcg ctgggcgcta cgcgcaactc
     1441 cagtacggtc acctgctcgc cctgcacctc gcgcacactc acttcttcgg ggatgtcaac
     1501 gagagccttg gcgatatcct ccactagttc cttcatccct gtcatccgtg atgctctcct
     1561 gaagccggca gaagcgttcg gcacggcatt cgtgcaagca taaggccccg cagccgacgc
     1621 ctattctgcc ttgatgatag cactcccaga cggccggact gaagaaactg aagcgtttag
     1681 gcgaccagct cggcggctgg agcaggattc gctttcagca gccgcgcgac agtatccgaa
     1741 ggcttcgcgc catttttcag ccagtgttct atccgttcgt gatcaaaatg tacggtcgct
     1801 ggttgtgtca ctgggttgta atagcccacc acctctacgg atcgactgtt gcgtgcacgg
     1861 tccttctcga tgactacgac ccgataagat ggtttctttt tggcgccaaa tcgcgccaaa
     1921 cgaatcatca gcatgtagat tccactcccg taatctcgtt acatccgccg tggtaaattc
     1981 ctccggcggt cactgctgct ccaagaaagg cagcttgagt tttcccaact tcttgcccat
     2041 gaatccgccg gaaaaggtct tcatcatctt ccgcgcctgg gcatactgct tcagcaaatt
     2101 atttacatcc tgaacggagg tgccgcttcc cttggcaatc ctccggcgcc gcgagccgtt
     2161 gaccaccata tggttggcac gctcatgcgg cgtcatcgag tcgatgatag ccacaatgcg
     2221 cgagatctcg ttctcgtcca ctttgacgtt tttcagatct ttcagaatgc cgacctgagg
     2281 catcatgcca aggatggatt cgagtgggcc gagtttcttc aactgcttga tctgattccg
     2341 gaaatcgtcc agggtaaact cgttctcgat gagcttccgc tgcatctcct cggcctgttt
     2401 ctggtcgatg ttttcttcca ccttctcaat gaaagaaaga acatcgccca ttcccagaat
     2461 ccgggatgcg gtccggtccg ggtggaacgg ctcgatggcg tccattttct cgcccgtccc
     2521 gatatacttc aacggctgtc cggtaacggt ccggatggaa agcgccgccc cgccgcgcgc
     2581 gtcgccatcc attttcgtca ggataacccc cgtgattcca agacgtttgt gaaactcgtc
     2641 ggcggatttc acggcgtcct ggccggtcat ggcatcggca acaaacagga tctcggtcgg
     2701 gttcagatgt tccttgaggc tttgcagctc ctccatgagc tgatcgtcga tatgcaaccg
     2761 gcccgcggta tccaccaata ccaggtcgcg gccggtttgc accgcctccc gccgagccga
     2821 ccgggcgagt tcgagggggt ccttttcctc cggcgcgccc gcatacaccg gcagcttcaa
     2881 gtccttgccc agcacgctga gctggtgcct cgccgcggga cgatatacgt ccacggaaac
     2941 catcagcgga ctgtgcccgg ttttcgaaag atgacgagcc agcttaccgg tagaagtggt
     3001 cttcccggac ccctgcaacc ctacgataag aatggccgtc ggaggctcgt tggcgaaccg
     3061 caacttggac tgatgggttc ccagtatctt gatcagctcg tctctgacaa ttttcaccac
     3121 ctgttgggca ggcgaaagcg ctgtcaggac ttcttccccc atcgccttct ctttcacgtt
     3181 ggcgatgagt tgtttgacca cccggaagtg cacgtcggct tcgagaagcg ccacgcggat
     3241 ctcccgcagg gcctcttcca tattttcggc cgtcagtttc ccttcgccgc gcagattctt
     3301 gaagacgcgc tgcagcttct cggataaatt ctcaaacatt gccggattcc gaactgtttt
     3361 gccaacccga tgtggggtta gagatcgagg gtcagtgtcc gcacactact gctcaagagg
     3421 cagtttccct attatagccg acagcgcgcc cggatgctgg cacatccgac ggcgtattcc
     3481 cgccgttaga agtttttccg ctccctctgg tacggctcct tcaagtattt ctgcacttct
     3541 tcctgtgccc cttgcgtgtt gtccttcgtc cgcgccgcaa gagtatactc gttcaccgca
     3601 cgatcacgct gtcccgtaac atcgaagatc ttgccaagat tcaagtgcgc ccaggcctcg
     3661 gtccatttcg gctccaggtc gccgttcaaa gcttcgcgga attcattcgc cgccgactgg
     3721 tagttgttct ggagaaagaa aacctcggct acccggtagt gcgccagaga gctgttccgg
     3781 ttcacgtcca gcgcacgctg gtactccttc aacgcttcgc cgaactcgcc tatttcggcg
     3841 aattgctccc cgcggcggat ggccacggcg acccgtacat catcgttgaa acgcaggatt
     3901 cgattgtgcg gatcgatctg cacccgcttg ggcttgccga aggtctcaac cacaaactcg
     3961 gaagacgtcc ccgacacttc gacgcgcttc tcctccgggt tcccttcggt ttcgattctc
     4021 aattccaccg gcattcggaa cgtgtccaga tcctgggcga tcttgcccat tacccggaag
     4081 cccttctgtg ttcgaaagat ggtgtactcc atgctgaact ccggtgcgcc gctcgactcg
     4141 atccactgaa tgaagaaata atccaggttc tggccggaga tcttctccgc cgctgtgcgg
     4201 aaatccgcgg tgctgacctc cttccaggca tgctcggatg gaaactcttt tatcagcctt
     4261 gtgaaattct catcgcctat gacatagcgc agcatattga gaaccgcagc gccttttgca
     4321 gcggtcgtgg cccagaattc aggggaataa tcctccagtc tcgacgactg gctgaccggc
     4381 agattctgaa cggtcaacgc ctcaatgtag acatcgcgca tttccgattc aagcgccggc
     4441 tgcccgttca agtgctccaa atacaacgcc tccgagtaac gtgcgaatcc gttcgacaac
     4501 cagatatgat tgcggttcac aggcgataga agtcctcccc accattggcg ggcagtctgg
     4561 ttggcgatca gccgcgcatt cacctgctgc ccgattccgc gcggtgagaa gaaaaggatg
     4621 cccggggccg agtaaccctg cggcgctccc agttcggttt ccacgaccgt caggttcgca
     4681 aacggcggca gcccgaaaag actcgtgagt tgagtcatgg ctttccctat ctcatcgcca
     4741 tacgcgcttg ccatgtcctt tcgcgtccgg aagtacaacg tcgtggtcac accctgcgat
     4801 gacaccgtgg agggctctcc gttcaccacg gcaatgctcc ccggaaacga aggctgcgtg
     4861 aacgaaacct gataacgcac acgatccggg ctggtgtgct cggccgtctc cgcgcctcca
     4921 gccactactt tgaacggcgt cggtacggtg attcgcatgt cggcggtata ccggtcggcg
     4981 gtatagttat tcaccggaaa ccagcgagcc gggtacatca ggtatgcgaa atcattctga
     5041 atggtggcga acttaatgcc ataaaccgga gacgcatctt ccctcccatt gaaatcaccg
     5101 tcataggtga acgtgagaga agacggctta cctttttcga gagccgtggg gagcgtcacg
     5161 cgaatggtaa agtcctgctg gttgcgcgac gccgggacag gacttccgtt ttcgtcgacg
     5221 acctgagaga cgttcatcgc gttgttcaat tcgaaaaccg cggaggaaag accctggtcg
     5281 accgctacga attgaacttg aacgatcgcc ttaatggact gggtgttcgg attgatttcg
     5341 gcgtcgatct tgtaatggcg aacatcgatt ccgggtcgcg tatcctgggc cggcgcgaca
     5401 gaggccatca gggctaccag cccaacgaaa caagcacggg tcatgttgta aatttccttt
     5461 gcaatagctc ctggacgatg tccgccgccc gctccatggg atctccatcc gtcctcagac
     5521 gggtagctac cttgcgcagg ccaaggcgca tttcacagcg agcactttca tcatccaaca
     5581 atcgttccgc ttcctgagcc agccgctctc cagttaactc ggattgcatc agttcgggga
     5641 ccacagcacg cccggctacc aggttgacca tcgtataaaa cggaacgcgt acgagaaact
     5701 ttccgagaac ccagcttgaa tggttcactt tgtaaaaagt gatcatcggg acccccaaaa
     5761 gggccgtctc cgtagcgatg gttccgctcg cggccagagc caggtccgaa tggccgacga
     5821 cgtcccacgt ctcgccctcg ataatcttga caggcgcacg gcctatacgg tctgcataga
     5881 atgagctgcc aaacttctga gaaaacccgg ccggcgcggc caacaagaag ctcaccgccc
     5941 tcctcccata cagccggttc accgcctcca ataccggagg caggtgcctg gaaatctctc
     6001 ccggccggct gcccggcagg atcgcaatta ctggacggtc aaggggaatt ctgtgtttcc
     6061 ggaaaaactc ccccttctcc agggaaggca taaccctccc cgccaacggg tgtccgatgt
     6121 aagtggccgc tacgttatgt tctttgaaga actgctcctc aaaaggaaag atgcataaca
     6181 ggcggtctac gttccggcgg agctgagata gccttcccct cctccaggcc catacctggg
     6241 gcgccaccag gtagaccacc ggcacccctt cgcgatgcag cctccgggca accggcagat
     6301 tgaagtccgg gctgtcggtg aggatcgcga tatcggggcg atgccgtctg gcataactca
     6361 tcaattcacg gaagcgaccg tagatccggg gcaggtgacg gatcacctcg acgaggccga
     6421 caacgctcag ctcgcccgca tccaggatgg cctggacgcc cgccgcctgc atacgccccc
     6481 cggcacagcc gtaaaattcg gcgcccggaa ggcgggaccg cagcgtccgg acgagactcg
     6541 ccgcatagct atcgcctgaa gcttcgccag ccgacacgaa tatgcgcaaa ctcatcctct
     6601 ctctgagcag gccggtgtca aattgcgccc ttctgctaca atatcaacag cctagatgtc
     6661 ctcccaaacc gatcttgcac ggcttgccgt ggaagacttc gtcgagcgct ttcgcggcga
     6721 agtggtcccc ctcagcaacc gcttttcgta cttcttcgta gcgttgccgc tcagcgagga
     6781 ggatacgcgg gaataccttc aggagcccat ctccgctttg cctccgaagc tgcttcccgt
     6841 tctgcctcgg atttcgatct tcctggttcc ttatcttgag agagccaacg ggcgcgagaa
     6901 aggtcatggt aagcccgaat acgtcgtctg tctcgacaaa cccgccgaac aacggcacac
     6961 ggtgatgtcg aaaatcacca ccgaagacga aacgactttc gtttttgcga tcaaggacca
     7021 ggaggtcgca gactaccact atcacctgta tcgcgcgctg gccgaggcgg ctgccgaacg
     7081 gtggagcatc gaggtgcaga accagttttt cagccttctt cgggaggaac tgggcgccaa
     7141 tgtgcatgga gaagtggacg aatcgagctg gcatgaaaag caggcgctgc tgcgccgcca
     7201 gacgaacgtc cggcgtgaaa cgaaagtatt ccgcagctat gcaaaacagg cgtttgttga
     7261 cacgctgacg ctctatctgc acggtatttg ctgcgatatc gatgtcgata ccgggccgcg
     7321 ccagcttccc agccgctacc tgcggcggcg tctgcaactg atgcagacct ttttcccgcc
     7381 gccgagcgga tacgcggttt tccccgaaga cctgaagccg gaatagcccg gaccgcttcg
     7441 tccttcaggc aaccgcgtcc gtgggctgat ccccgctcaa tgccttaagt atctgcagtg
     7501 cgatctcctt cccgcggcta cccgcctcct ttgccgggcc cacacaactg gggcaccctg
     7561 cttcgcacgg acaggcctcc actacctggc gggcgctgga gaccagcgtt cgagccatcc
     7621 ggagcagagg cacgctctgg ccgatgccgc ccgggaagtt gtcgtacagg tgcaagtccg
     7681 gctcaaacga cacggtgccc ggcgaattgt cctcggttac cgctacgccc agatcgcgcg
     7741 gatcgcacat cagcagcaga cccgcgaccg atcgcagtac gttggcggct ccggctaaac
     7801 cctcctgccg ctcggtgggg cttacacctt cgaagggagc gaagaaagaa gcgggaaaat
     7861 gcagccagaa cgacgtggtg tgcatctcct gctccggcat gctcaggttt ccggccccca
     7921 cattctcgtt ggtgtggaac ttgattttct tgaagccgac aatctgccgg tttacacgga
     7981 catcgccatg cgcggccgtt gccggcgcca gcggagagca ttccacctgc tcgaggatct
     8041 ttacctgcgt gtaatcgatg gcatccgtgt aatactcgca ctcgacgcgg cggacatagg
     8101 ctttgcggtt ctcgtagtcg aaacgctcca cttcgtactg ccgcgcctcg tgaagataga
     8161 tggccttttc atgcaggctg gttaacgccg tggggaacga cacctccgcg atgacctgcg
     8221 gctcgcccgt cgtatctaca actacaaaat tgtcgcttgt caccgcgcgc aagctgatcg
     8281 catccgcggg gtaggtgtcg ctggtccagt gccagcactc gccggagtgg tgaagtactc
     8341 ctgtctcggc cagaaagcgg cacaactcgc cggtggcatg cgggccgaac ttttctccgt
     8401 cccggatagg aagctcgaaa gccgcacact tcaggtggtt cagcaaaatc tcgaggttat
     8461 cgggattgat gtaagcctgt tcgggtgatt ggccgaaaaa ataatccggg tgttcgacga
     8521 tgaactggtc cagggaattg ctggaggcca ccagcagggc cacggaagcc gtgttgcgcc
     8581 tcccggcgcg cccggcacgc tgccacgtag acgcaatcgt tccgggatag cccgccatca
     8641 ccacacaatc gagcgatcca atatcgatgc ctaactccag ggcgtttgta gccaccacgc
     8701 cacggatccg gccttcccgg agttcacgtt cgatctcacg cctctcgcgc ggaaggtatc
     8761 cacccctgta tccgcggatc gaagccggcg gataggtccg ccgctcgcaa tcgtgcttca
     8821 gatacgtgag caggacttcg gtggccaggc ggttgttggc aaaaacgagc gtctggtgtc
     8881 cgtgactgat caactcgcct gcaatacggc gcgactcgcc caggtaactc ctcctgatgc
     8941 cgagttgatg attgaccatg ggcgggttgt aaaagatgaa atatttgtcg ccgcccggag
     9001 cgccgttcga gtcaatacac tctactcgcg attcggttaa tgcttccgcc aattccgccg
     9061 gattggcgat ggtggccgag cagcaaatga actgtggaga ggacccgtaa aactcgcaga
     9121 tacgcttcaa tcgccgcagt atattagcca ggtggctccc gtaaataccc cggtagtaat
     9181 gtaactcgtc aataactaca taccgcagac gctcgaaaca cttagcccag cgggtgtgat
     9241 gaggaaggat accgctgtgc aacatgtcag ggttggtgag gataatattg gcccgctcgc
     9301 gaatggcttt tcgcgcgtcc tgaggagtgt ctccgtcgta agtgtgagcg ccgattcctg
     9361 caccggaagc gtcgatcgtt gcctttaatt cggaaagctg atcttccgcc agcgctttcg
     9421 tcgggaacag gtacagcgct cgaatgccgg gatcggataa aaggctgttc agaaccggga
     9481 ggttgtagca gagggttttg ccgctggcgg tgggcgttac gataaccaca tgccggccgc
     9541 tctctacggc gttgaacgcg tccgcctggt ggctgtagag tttctcgata ccgcgggcgg
     9601 ccaggacctg ggacaagaca ggcgacaccg cttcc
//
LOCUS       DASTMK010000315         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_399077, whole genome shotgun sequence.
ACCESSION   DASTMK010000315 DASTMK010000000
VERSION     DASTMK010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399077"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /gene="purF"
                     /locus_tag="VFQ79_13755"
     CDS             <1..1432
                     /gene="purF"
                     /locus_tag="VFQ79_13755"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU0140781.1"
                     /translation="MPELAFDKFHEECGVFAIYGHPEAANLTYLGLYALQHRGQESAG
                     IASSDGREIYTSKEMGHVADVFTPERLASLRGDLAIGHTRYSTAGDTALVNAQPFSVI
                     CNKGPVAVAHNGNITNAIELRSALERDGSIFQASSDTEVILHLMARSRERTLSGALRD
                     ALLQLEGAFSLVVLTKDHVLVARDPHGFRPLAIGQMEMSGGRFAYVFASETCAFDLIG
                     AIYLDDVQPGEMVIAGPDGMTRERYSPMGRRAHCVFEHVYFSRPDSIVFGRAVQQSRE
                     NLGRLLAQEHPADADVVVPVPDSGVTAALGFAAESGIPFRHGLIRNHYVGRTFIEPSQ
                     AIRDFGVKLKLNPVRHLLEGKRVVLVDDSIVRGTTSRKLVRMVRGAGAREVHLRISCP
                     PTVSPCFYGVDTPTKKELIAANISVEEIRKFVEADSLGYLSLESLRDAVGDYRDEYCY
                     SCYTGEYPTSDLVTVEALMQSKPFRR"
     gene            1638..2441
                     /locus_tag="VFQ79_13760"
     CDS             1638..2441
                     /locus_tag="VFQ79_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HEU0140782.1"
                     /translation="MPYQIVVCGSIVPDPLQTLEPVIGPGGPSLKNEMLLPSVLDPWA
                     AHALYEAAHLASTVAGSKVWLVSLGPKAKLQQVVMTIAQKAPFELIALDGSASGFTDA
                     HETAKAIATAIQAIPDLDRSRLLVFGGWESASRGAGVTMQIAGELLGIVDQFQGVDEL
                     KIAEDGRLRVLERVEEGKHQVSVCDGPPALLGWATGNRPEPRNNPQVGMLNMRTAMPA
                     IQKARPAAAGAGDILFQGVALPKQQRETEIVKDRSAEEIAREITAWIRA"
     gene            2452..>3311
                     /locus_tag="VFQ79_13765"
     CDS             2452..>3311
                     /locus_tag="VFQ79_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HEU0140783.1"
                     /translation="METTLFLAHTEADGSLGKIALESLGAAKELAGDSLVAGVIGENT
                     SVAASLPASRVFTITGTEFAQARYATDAAAAEAVCRTSGAAIVIAPATSRWARALPGV
                     AHRLGGRADTHVTGIVLREGKLFATRWYYRQRMEGTFERSGRPWVLLLESGCHAPWSG
                     AAGAGTVEAVEVPLAEASRRTAVQGVQEPQAGQQTIRPDAELLFVTGAGWTKAQRDRK
                     PHVKEAETLILEFLANTQASLGSSKSLVDISGEGQQVLEFLTHMNQVGQTGSTPRHPK
                     GLATCCHGEEP"
BASE COUNT          652 a          961 c         1052 g          646 t
ORIGIN      
        1 catgcctgag cttgcttttg ataaatttca cgaggagtgt ggcgtgttcg ccatctacgg
       61 ccatccggaa gctgctaacc tgacctatct cggactttac gcgctccaac acagaggaca
      121 ggaaagcgcc ggcattgcca gcagcgacgg ccgcgagatc tacacttcga aggagatggg
      181 gcacgtggcc gacgtcttca cgccggagcg attggcgagt ctgcgcggcg atctcgcaat
      241 cggccacacg cgctattcca ctgccggcga tactgcgctg gtcaatgccc aaccgttttc
      301 ggtcatctgc aataagggcc cggtggcggt agcgcacaac ggcaatatca ccaatgcgat
      361 tgaactgaga tctgcgctcg agcgggacgg ctccattttc caggcgtcga gcgataccga
      421 ggtgattctc cacctgatgg cgcgctctcg cgagcgaacg ttatccggcg cgttgcgtga
      481 tgcactgctt caactggagg gcgcgttttc cctcgtcgtt ctcacgaaag atcacgtttt
      541 ggtagcgcgc gacccgcatg gttttcgtcc gcttgccatc ggacagatgg agatgtcggg
      601 cggccgattc gcctacgtgt ttgcatcgga aacctgcgcg ttcgatctga taggggccat
      661 ctatctggac gatgtccagc ccggtgaaat ggtgattgcc ggtccggacg gcatgacccg
      721 ggaacgctat tcgcccatgg gccggcgcgc tcattgcgtg ttcgaacatg tgtatttttc
      781 ccgcccggat tccatcgtat tcggccgggc ggtgcagcaa tcgcgcgaaa acctgggacg
      841 cctccttgcc caggagcacc cggcggacgc cgatgttgtt gtgcccgtac ccgattcggg
      901 cgttacggcc gcactcggct tcgcggctga atccggcatt ccgtttcgcc acgggttgat
      961 ccgcaatcac tatgttggcc ggacgttcat cgagccgtct caagccatac gcgatttcgg
     1021 ggtcaagctg aagctgaatc ccgtgcgcca ccttctcgaa ggaaagcgcg tggtactggt
     1081 ggacgattcc attgtgcgcg gcactaccag ccgcaaactg gtgcgaatgg tgcgtggcgc
     1141 cggcgcgcgc gaagtacacc tgcgcatttc gtgcccgcct actgtttcac cgtgctttta
     1201 cggcgtggac actcccacga aaaaggaact catcgccgcg aatatcagtg tcgaagaaat
     1261 ccggaaattc gtggaagcgg acagcctcgg atacctgtcg ctcgaatcgt tgcgggacgc
     1321 ggtgggcgat tatcgcgatg aatactgcta ctcgtgctat acgggagagt atccgacgtc
     1381 ggatttggta accgtagaag cgctgatgca atccaagcca tttcgccgct gaacagaact
     1441 gcgggcgtgg agccggcaaa cggtatacgg cttgtagctg agccgtgagc gttagcgagc
     1501 ggttggtggg gcatcggatc gcttcccgct ctgcaaaatc aggtctaccg ctccgttgcc
     1561 gtcgcggctc agttaccgat tctcctgcca ctccatgtcg cttgaatcta gatattgtgg
     1621 tctggttata ctgagatatg ccctatcaga tagtggtctg cggcagcatt gtgcccgacc
     1681 ctctgcaaac gctcgagccg gtcatcggac ctggcggtcc ttccctcaag aatgagatgc
     1741 tgctgccgtc cgtgctcgat ccctgggccg cgcatgcgct gtacgaagcc gctcatctgg
     1801 cgagtactgt tgcgggcagc aaagtgtggc tggtcagtct gggtccgaaa gctaaactcc
     1861 agcaggtcgt catgaccatc gcccagaaag ctccatttga gttgattgcg ctggatggat
     1921 ccgcaagtgg cttcaccgac gcacacgaga cggcaaaagc aatcgccacg gcgatccagg
     1981 cgatcccgga tctggaccgc agccggctgc tggtcttcgg cggttgggag tcggcttcgc
     2041 gcggcgcggg cgtgaccatg cagatcgccg gcgaactgct ggggatcgtc gatcagtttc
     2101 agggcgtgga tgagttgaag atcgccgaag atggccgctt gcgagtattg gaacgggtgg
     2161 aagagggaaa acaccaggtt tctgtctgcg acggcccgcc ggccttgctg ggctgggcga
     2221 cgggaaaccg gccggagccg cgcaacaatc cgcaagtggg catgctcaac atgcgaaccg
     2281 caatgcccgc catccagaaa gccaggcctg cggccgccgg cgctggagac atactttttc
     2341 agggcgtggc gctcccgaag cagcagcggg aaaccgaaat cgtgaaagac aggagtgccg
     2401 aagagattgc gcgcgagatt accgcgtgga tacgcgcata gagaggcgac gatggagacg
     2461 acactttttc ttgcacacac ggaagcggat ggttcgctgg ggaagatcgc cctggagtcg
     2521 ctgggtgcgg caaaggaact ggcgggcgat tcgctggttg ccggagtcat cggggagaac
     2581 acttccgtgg ccgcgagcct tcctgcatcg cgagtattca cgatcacagg aactgaattc
     2641 gcgcaggcgc gctacgcaac ggacgccgcc gccgccgagg cggtgtgccg cacatcggga
     2701 gccgccatcg tcatcgcgcc ggccacttcc cgctgggcga gagcgctgcc cggagtggcc
     2761 caccggttgg gaggccgcgc ggatactcac gtgacaggca ttgtcttgcg cgaaggtaag
     2821 ctatttgcca cgcgctggta ttacaggcag cgcatggaag ggactttcga gcggtccggc
     2881 aggccgtggg ttcttctgct cgaatcgggc tgtcacgcgc catggtccgg cgccgccggg
     2941 gcgggcacgg tagaggcggt ggaagtgcca ctggcggaag cgagccgcag aactgccgta
     3001 caaggtgtgc aggagccgca agccggacaa cagaccatac gtccggacgc cgaactgcta
     3061 tttgtcaccg gtgcgggctg gaccaaggcc cagcgggata gaaaaccgca tgtcaaagag
     3121 gccgagactc tcattctcga gtttcttgcc aatacgcagg cttcactcgg aagcagcaaa
     3181 tctctggtgg atatcagcgg cgaaggtcag caggtgctcg aattcctgac tcacatgaac
     3241 caggtagggc aaaccggatc gacgccgcgg caccccaaag gcttagccac ctgctgccat
     3301 ggagaagagc c
//
LOCUS       DASTMK010000316         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_142620, whole genome shotgun sequence.
ACCESSION   DASTMK010000316 DASTMK010000000
VERSION     DASTMK010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142620"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFQ79_13770"
     CDS             complement(<1..495)
                     /locus_tag="VFQ79_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140784.1"
                     /translation="MLLVTRSVIAGATLLLLLVQPGSSQTGKGLGQSVKGAVFVDQKN
                     GYFTMIPPSGWPRQTYDDTRTKVAWRSPQDTRVLLSVIAKQATESFSDMRAGADRTAQ
                     SWRSKGVSTEVSEGRVGDVPAVIIKANVPGAGISHLLLFLVQGIHFNVQFAAPDRELF
                     DRHLP"
     gene            complement(591..1022)
                     /locus_tag="VFQ79_13775"
     CDS             complement(591..1022)
                     /locus_tag="VFQ79_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140785.1"
                     /translation="MPVFRVAALIIIAAVWVLPLPAQVSGGSPHAAALLPGLPTAIPA
                     ANAGWRRVVYRWDNPRESMPDSVWVSRAVSEGQRSKGKIALGLGLIGGGLALMIANPD
                     VSETFMDSSGRMYKKSVRMRLSGGIIAAGGGFFLWQGLKKN"
     gene            complement(1058..2854)
                     /locus_tag="VFQ79_13780"
     CDS             complement(1058..2854)
                     /locus_tag="VFQ79_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140786.1"
                     /translation="MKIHNFRLYACFAVGLFLTGPIRAVEPARQWYAVTLDPPSVVII
                     NAVKPSVTATIELAGVPSFVATEPGNRFLYVLLDGWFDLSQQWHKQSADSELAVIDLA
                     ERRVVKSIPVGWKVSSIEASQDGKYLLCLSVGRPPENEVIEQPALITVVETASTEVAA
                     VFTGPLTEMRYRIDKDNPGELAAMRFTPDLERIMLLHFGIRESGKTAAIPASVTFFTL
                     GKKEPTRVVQLKHAGGKGELSPSGRWLYMVNTGNQAIRQKNMRTGAVHVIDVESGAEV
                     ARHRLGYQPRALAADPQLDVVWVLSHDTDYRKPGTVHRLRGDKVQLFRTGPGPFFNVR
                     PEGLNGRVVGSSADLLYIPDEQPESSRLIRFRNYKDTPKLSKTWKGTPAADLVYLPGS
                     QRLVAHSALGNEAAIFDLESAVFVQSFKTGRGGVKFAKAMGRFGLAMGASMAYAGAFW
                     AVGSSDYIIFVPGGQPRTSIAAGPGGHNVYLLNSEANDVTVVESGTGRTIGKIAVGGG
                     AQNLFASPDRRFICVGAEGHIALIDTHTNKLHAKQDVDGKVTWFGLTPDGGSLAVLTT
                     RGAAILDASEGKILASLDGVAGAHLLTGPRPQ"
     gene            complement(2954..>5188)
                     /locus_tag="VFQ79_13785"
     CDS             complement(2954..>5188)
                     /locus_tag="VFQ79_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140787.1"
                     /translation="SRPSMLLNWIPLAGGSTRHRKDPASGEVPARQIFEQLDRMLQSR
                     SFCNSRDLTRFLRWVVERKLAGETDRLKEQVIGVEVFGRPTDFNPATDTIVRTQASNL
                     RKKLATYYQTDGLLDEVLILLPPRGYVPRIEHRASVQEAHPAFWRRRLWPLVFGVFAI
                     IVLAMSAVAFWRYPKPHWTVQRLTFDNQSTWPAISRDGRFVVYSSPRADRANSDIWWH
                     PLDGSGPRQLTSYPAIDTTPDISPDGSMVAFRSWRDGGGIYVVPRSGGAERLLVPRGF
                     SPRFSPDGRWIAYAASTGKGGSSIFLIRPSGGQPHNILADVEGALYPLWTRDGKRIIY
                     IGLLHDGASEKEEYDWFITDVDTGSPAPGRSVAMGVSEMLRKQGFPDVSSSTIPMDWN
                     GAAILFNFKNQRISELWQLPISPETWKPTGPASRLLLGPELQYARSASVGGARILAAA
                     TDRQTNDLWSVPLSSGVPRQVTRDESLRQGMAGTIPRVTADGNKLIFPSERSGHLDLW
                     LKDLPTGNATRLTSRPGTKNRPAITPDGTTVAWNQGESGSRSLHVMDIRTGTPRWVCS
                     DCGDLVDIASDARSALTISRGRLSRIGLANGQSSEILNNPAITPVHASFSPDIKWVAF
                     AAFRSGEDPVKGFIAPLADPLPGEDSWIRLTEAPHHLSFAWSPDGNSVYYFSMQDGFR
                     CLWSQTLNPSTKRPVNSPRAVCHFHKDSPYPWFGSWIAAAHDKIILNLSTTSSDVFIA
                     KRPF"
BASE COUNT         1114 a         1541 c         1488 g         1045 t
ORIGIN      
        1 cggaagatgc cggtcgaaca gttcccgatc aggagcggcg aactgaacat tgaagtgaat
       61 gccctgcact aaaaagagaa gcagatgtga gatgcctgcg cctggcacgt tagctttgat
      121 gatgacagcg ggaacgtctc ccaccctgcc ttcgctgacc tccgtagaca cacctttcga
      181 tcgccaactc tgtgcagtgc gatcagcccc ggcgcgcata tcgctgaagc tctctgtcgc
      241 ctgtttggca ataacggaca gaagtacacg cgtatcctga ggactcctcc aggctacttt
      301 tgttcgcgtg tcatcgtagg tctgccgcgg ccagccagac ggcgggatca tggtgaaata
      361 tccattcttc tggtccacga acacggcccc cttcaccgac tgtcccaggc ctttgccggt
      421 ctgtgagctg ccaggctgaa ccagtaacaa caacagagtg gcaccggcaa ttaccgaacg
      481 agtaacaagt agcatttttc tatcaactcc tttccgtaag agcctcgcac cccggcgaag
      541 aggacaggac gcggcgccct ccgcgccgcg tctccatcat cgtccggcgc tcaattcttc
      601 ttcagtcctt gccagagaaa gaagcctccg ccagcggcta taatgccgcc ggaaagacgc
      661 atacgaacgc tctttttgta catccggccg ctcgagtcca tgaaggtctc cgaaacgtcg
      721 ggattagcga tcatcaatgc aaggccgccg ccgattagtc ccaacccgag cgcgatcttg
      781 cccttgctgc gctgcccttc actcacggca cgcgaaaccc aaacactatc cggcatgctt
      841 tcccgtgggt tatcccatcg ataaacaacg cgccgccaac cggcgttcgc ggcaggaatg
      901 gcggtaggaa gaccaggaag cagtgccgcc gcgtgaggcg agcccccgga tacttgcgcc
      961 gggagaggca acacccaaac cgcagcgatg ataatcagag cagcaactcg aaacacaggc
     1021 atgattttcc tctttctgtt gtgtttgaaa aacgcatcta ttggggccgc ggaccggtca
     1081 gcagatgcgc accggcgacg ccgtccaatg aggcaagaat cttcccttca gaagcatcca
     1141 aaatggccgc tcccctggtg gtcagcactg ccaggctgcc gccatccggc gtcagcccga
     1201 accaggttac tttcccatcc acgtcttgct ttgcgtggag cttgttcgtg tgcgtatcga
     1261 taagcgcaat atgcccctcc gcaccgacgc agatgaaccg tcgatcggga ctggcaaaga
     1321 ggttctgggc gccgccgccg accgcaattt tgccgatcgt gcggcccgtc ccgctctcca
     1381 cgacggtgac gtcgttggct tcagaattca ggaggtagac attgtggccg ccgggtcccg
     1441 ctgcaatgct ggtgcggggc tggccacccg ggacgaatat gatgtagtcg gatgatccca
     1501 ccgcccagaa tgcacccgcg taggccatcg aggctcccat tgccaacccg aaccggccca
     1561 tcgccttcgc aaacttcact ccacctctgc ctgtcttgaa agactggaca aaaaccgcgc
     1621 tttccagatc gaagatcgcc gcttcgtttc cgagggcgga atgcgccacg agacgctgtg
     1681 agccgggcag gtacacgagg tcagcagcag gagtgccctt ccaggttttc gataactttg
     1741 gagtgtcctt atagttccgg aatcggatca ggcgcgacga ttcgggctgc tcgtcgggaa
     1801 tgtacagaag atccgcgctg ctgccgacaa cccggccatt caggccttcc ggacggacgt
     1861 tgaagaacgg tcccggacca gttctgaaca actggacttt gtctcctctc agccgatgga
     1921 ccgtgcccgg cttgcgataa tctgtgtcgt gactgagcac ccacacaaca tccaattgcg
     1981 gatcggcggc caaagcgcgc ggttggtagc cgagtctgtg ccgcgcgacc tcagcaccgc
     2041 tttcgacatc gatcacgtga accgcaccgg ttcgcatgtt cttttgccgg atcgcctgat
     2101 tgcccgtatt gaccatgtag agccagcggc ccgatggcga caactctccc ttgccgccgg
     2161 cgtgttttag ctgaaccacc cgagtaggtt cctttttgcc gagtgtgaaa aaagtcaccg
     2221 aggctggaat ggcggcagtc ttcccgcttt cgcgaatgcc gaagtgcagt agcatgatcc
     2281 gctccagatc cggcgtaaag cgcatcgcag ccagctcgcc cgggttgtcc ttgtcgattc
     2341 ggtagcgcat ctcagtcagc ggtcccgtga acacggccgc aacttcagtg gaagccgttt
     2401 cgacaacggt gatgagagca ggctgttcga tgacctcgtt ttcaggaggc cggccaacgg
     2461 aaagacatag gaggtatttg ccgtcctgcg aagcctcgat cgaggaaacc ttccagccga
     2521 ctggaatgga cttcaccacg cgcctctccg ccaaatcaat gacagcgagt tcgctgtcgg
     2581 cgctttgctt atgccattgt tgagagagat cgaaccaacc gtccagcagg acgtacagaa
     2641 agcggttccc aggctctgtc gcaacgaaac tcggaacacc cgcaagttct atggttgccg
     2701 tgacggacgg ctttactgcg tttatgatca ccactgaagg cggatcgagc gttacggcat
     2761 accattgacg agctggctct acggcccgaa tgggtccggt gagaaaaagg cccactgcga
     2821 aacacgcata cagccggaag ttatgaatct tcatcggtca tactccccgg aattacaaga
     2881 actacgacat gcagtatggg tgggtatcag ccgccgtcca agttaaaaca gcgttaagac
     2941 cgtgtgttat cggttaaaaa ggccgtttgg ctatgaaaac gtcggaggaa gttgtggaaa
     3001 gattcaagat aattttgtcg tgggccgccg caatccaact cccgaaccag ggatacggtg
     3061 agtccttatg aaagtggcac accgccctcg gtgagttcac aggtctcttt gttgaggggt
     3121 ttagagtctg gctccagaga cagcggaacc catcttgcat cgagaagtaa tacacgctgt
     3181 ttccatccgg cgaccaggcg aacgagaggt gatggggcgc ctcggtgaga cgaatccagg
     3241 agtcctcgcc cggcaacgga tcggccaggg gggcgatgaa tcccttgaca ggatcttctc
     3301 ccgaacggaa tgcggcgaag gcgacccact tgatatcggg ggagaagctc gcgtgaaccg
     3361 gcgtgatggc ggggttgttg aggatttcgg acgactgccc attggcaagg ccgatccttg
     3421 atagccggcc gcgcgagatg gtgagcgccg atcgcgcatc ggatgcgatg tccacgagat
     3481 caccgcagtc gctacacacc caccgtggag tgccagtgcg gatgtccatc acatgcaggg
     3541 atctgctccc gctctcgccc tggttccagg cgaccgtcgt gccgtcaggt gttatcgccg
     3601 ggcggttttt ggttccgggt cgcgaggtca gacgtgttgc attcccggtc ggcagatcct
     3661 tcagccagag gtcgaggtgc ccggaccgct ccgaaggaaa gatcaacttg tttccatcgg
     3721 cagtgactcg tggtatagta cccgccatcc cctgacggag ggactcgtcc cgagtcactt
     3781 ggcgcggcac cccgctggat agaggtacgc tccacaaatc gttggtctgc cggtcggtgg
     3841 cggccgccag gatccttgct ccgccaacac tggccgatct ggcgtattgg agttccggac
     3901 ctaaaagtaa tctcgatgca ggtccggtgg gtttccaggt ttcaggagag atcggaagct
     3961 gccaaagttc cgaaatgcgc tggttcttga agttgaacag gatggcagcg ccgttccagt
     4021 ccatcgggat cgttgaactg gagacatccg gaaagccttg cttgcgaagc atctcggaaa
     4081 cacccatggc caccgagcgg ccaggggccg gcgacccggt atccacgtct gtaatgaacc
     4141 agtcgtattc ttccttttcc gatgcgccgt cgtgcaggag cccgatgtaa ataattctct
     4201 tcccatcgcg tgtccagagc gggtaaagag cgccctcgac gtccgcaagg atgttgtgag
     4261 gctgcccgcc ggatggccga ataaggaaaa tgctgctgcc acccttaccg gtggaagcgg
     4321 cgtaggcgat ccacctgccg tcgggtgaga atcgtggaga aaaaccgcga ggtaccagaa
     4381 gccgctcggc acctcctgac ctcggtacga cataaatacc gccgccatca cgccaggagc
     4441 ggaaggcgac catactcccg tcaggcgaga tatccggcgt cgtatcgatc gccggatagc
     4501 tggtgagctg tcgcggaccg ctaccgtcta aagggtgcca ccagatgtcg ctgtttgctc
     4561 gatccgctcg cggcgatgaa taaactacga aacgaccgtc gcgcgagatg gctggccaag
     4621 tcgactgatt gtcgaaagta agccgttgta ccgtccaatg aggtttcgga taccgccaga
     4681 acgctacggc cgacatcgcc aagacgatga tcgcaaagac tccgaatacc agcggccaca
     4741 gcctacgacg ccagaacgcg ggatgcgctt cctggacaga agcccggtgc tcaatccgcg
     4801 gcacatagcc tctgggcggc agcaaaatga gtacttcgtc taacaagcca tctgtttgat
     4861 agtaagtagc cagtttcttg cggaggttcg aagcttgagt ccttacgatg gtgtcggtag
     4921 ctggattgaa gtcggtgggc cgcccgaaca cctcgactcc aatcacttgc tccttgaggc
     4981 ggtcggtctc tcccgcgagt ttccgctcca caacccagcg cagaaagcgg gtgaggtcac
     5041 gcgaattgca gaatgaccgg ctttggagca ttcgatccag ttgttcgaag atctgacgcg
     5101 ccggaacctc tccactcgcc gggtccttcc gatgccgggt actcccacct gccagcggga
     5161 tccaattcag aagcatggat ggtcttga
//
LOCUS       DASTMK010000317        10131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_206734, whole genome shotgun sequence.
ACCESSION   DASTMK010000317 DASTMK010000000
VERSION     DASTMK010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206734"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VFQ79_13790"
     CDS             <1..1170
                     /locus_tag="VFQ79_13790"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140788.1"
                     /translation="QNPSGLEYVQALGSLAETLVSQNRLEEAEKSVARMREAGAADTR
                     NGKFYEVMAVQVLARIRDLQKKPEEARQLRASMGGRDPMAANREKTVYEATASAQQTA
                     LQGDIDAAVATAGEALALAAERSRSNPQEVSGLMSLANVLMDKQKKEEARRIMIETLR
                     ILDRVPDHPRVADALGSVTSVLFRLGMAAEAERAAERQEKILLEAKGAESLVLHSISH
                     ARIERMQRDGDWMGVMEERKRMLARVEKMTGTKSRESLSALREVAWAYPQLNNWPEEE
                     RVLSALLERTANLSGRASVDHAHLLVQMANRAGHNRQFEKALNWIDQAIEVARALPDA
                     DVHLPGIMQNRAQIAQAKEAPRGGPFYAPAGAASGAGRWFDTDRFRQSGPTVPNK"
     gene            1222..2058
                     /locus_tag="VFQ79_13795"
     CDS             1222..2058
                     /locus_tag="VFQ79_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU0140789.1"
                     /translation="MKALLEILQPGEPDWDLAQAIDPDRLPAHIAIIMDGNGRWANRR
                     SMPRVAGHRAGIGPVRATVETCARLGIEALTLYAFSIENWKRPRQEVDTLWRLLRHYL
                     GKEMASLMRNGIRLTAIGRLDSLPPDVRRDLDQAVRQTEANNGLRVNLAINYGGRAEL
                     VDALNALLEEARLEGRLSTLQIDEEAIEAHLYTAGQPHPDLLIRTSGEMRISNFLLWQ
                     IAYSELYVTDVFWPDFSRSDLLRAICEYQKRDRRFGGLSPRGHGRSSRQPVAEEIGVP
                     AR"
     gene            2055..2825
                     /locus_tag="VFQ79_13800"
     CDS             2055..2825
                     /locus_tag="VFQ79_13800"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU0140790.1"
                     /translation="MKRVLTALALIPFILFVIFWGPSWLFLAVVVTVAALCFYEYSGI
                     VEGHGIARPGPAGYAAGLLFLLVQRNEALLIVLVALLIAALSLRKTELSKCLPYAAAL
                     MFGVLYAFGPWRSALALRSVDPLWLFLPLLVSWTGDTGAYYVGRVIGRHRLAPRISPA
                     KSWEGAVASVITAAAVGTLYCAYLLPSVPLIEAVGLTAVTNVAGQIGDLAESAMKRGA
                     GVKDSGTTLPGHGGWLDRVDSALFAIPVVHFYLWRGQF"
     gene            complement(2836..3246)
                     /locus_tag="VFQ79_13805"
     CDS             complement(2836..3246)
                     /locus_tag="VFQ79_13805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0140791.1"
                     /translation="MIEYRVGNDLDLDQVIELYKASTLGERRPVEDRERMRTMLRTAN
                     LTVTAWDGDLMVGIARSLSDFAYVTYLSDLAVRLSHQRQGVGRELIRITRRESGPEAY
                     IVLLSAPKAVEYYPRIGFKPHPSAWILRADDTLL"
     gene            complement(3243..3512)
                     /locus_tag="VFQ79_13810"
     CDS             complement(3243..3512)
                     /locus_tag="VFQ79_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0140792.1"
                     /translation="MEHTCKEIFSMLSQYLDAELPESTCEEIRQHIHGCPPCVQFVES
                     LKRSIDLCHEYSSGEVPRPIREEVRRELLGAYRKMLSAREGASGL"
     gene            complement(3517..4122)
                     /locus_tag="VFQ79_13815"
     CDS             complement(3517..4122)
                     /locus_tag="VFQ79_13815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0140793.1"
                     /translation="MHQTDDDVDLARDLLSGKEEAFDRFLERFRTKIFQYSYLMCGQR
                     EDAEEVSQETLLKVFEKLDQLKEPENIRAWVFRIAKNACLMKRRKSVFAPERELSLEE
                     LIPAGDGEGVERKLQLADWSALPETQALNSELRAVLERAIGELPEIYRSVILLRDVEE
                     LTTEEAAQVLEVGTDVVKTRLHRARLAVRKKLDGYLQSARG"
     gene            complement(4158..4577)
                     /locus_tag="VFQ79_13820"
     CDS             complement(4158..4577)
                     /locus_tag="VFQ79_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0140794.1"
                     /translation="MDVTVRYRNGVQFQAETRGHSVICDQPSGNGGADEGMSPPEFLL
                     VSLGTCAGYYAAQYLKARNLPMEGLEVRVRAAKDGKPVRLDSFVIEVTVPGVDGERHR
                     EGLLRATKLCLIHNTLSHPSQIDIAIGFPLGTVVQQL"
     gene            4685..6019
                     /locus_tag="VFQ79_13825"
     CDS             4685..6019
                     /locus_tag="VFQ79_13825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0140795.1"
                     /translation="MRPFTLFALLCCTIPLPAQDSLSLQEAVETALGRHPAIEASASN
                     TRAADARIRQARSGYLPKLDYSEAYQRSNNPVYVFSSLLTQQQFAVENFEIGRLNQPD
                     ALNNFQSLLTADQILYDAGRTKKQVEAAEIGRNLASEDERGARMSVILGVVRAYYGAV
                     LANEGLQVAREAVRSAEADLERAEAVRQAGMSTDADVLSIRVHLAAMREQEIRRVHEL
                     DVARAALNEALGLPLETPHQLTTPLAAASVAYAPELEKSALSERPEARQADLRSRLAR
                     TQADGARAAYFPQVFARGVFEADRQRFVTRGGANWMFAAGLRWNLFNGFADRARIEEA
                     GHAAAAAQSQQEQTSRAVQLQVRGALSNLSAAEERIKVAEAAVGMAEESLRITKNRFD
                     NGLSTVTDLLRNETALLEVRMRRLSAIHDQRVAAAAVERAAGTLSTASNVLR"
     gene            6037..7182
                     /locus_tag="VFQ79_13830"
     CDS             6037..7182
                     /locus_tag="VFQ79_13830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0140796.1"
                     /translation="MTRMIPIAVLLLSLAACGHDSGKKSQTPPAAPVTVKVLTVTGTD
                     LPAVYEAVGTVKARTAATISSRVMGYVRDVRVAVGDRVRAGQLLVTIDSRELEAVYGQ
                     AEAARAEAQSAIAEVENSVSAAKANLELSQVTFNRMKDLFEKRSISNQEYDEAAARLE
                     VAKANLQMAQSKRTQLAAKVRQADAAVRSAGIVRGYAQITAPFAGVVTEKHVDPGNLA
                     SPGAPLLTVEQAGAYRLEVPVEESRLGSIQSGRPVEVVLDALNQRLQARVSEIVPAID
                     AASRTFLVKIDLPRIAHLGSGLYGRAVFQSGVRRSIVVPADAVIQRGQVLSVLVADDG
                     IARTRLVTTGRERDGRIEILTGLVQGEKLIYPVPPSVLDGARVEVQP"
     gene            7179..>10131
                     /locus_tag="VFQ79_13835"
     CDS             7179..>10131
                     /locus_tag="VFQ79_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0140797.1"
                     /translation="MNSSKLGVAGRFARLWMESKLTPLFIVASVLLGAFSVWKLPREE
                     EPQIIVPMIDIFVGMPGASAKEVEERVTRPMEKLLWEIPGVEYIYSTSSPGGALAIVR
                     FRVGEDEEKSIVMLNQKMYSNFDRIPPGATQPLIKPRSIDDVPILALTLWSRRYSGFE
                     LRQVAAQVHDAISQVNDVSTVNLIGGERREIRVVLDEAKLSAYSMTPVQIIAALGASN
                     RRLESGRYSTGNRELLLETGEFLQTAEDVRNVVAGVANQRPVFLRDVAEVIDGGEEPS
                     QYVHYASNSAFHPAVTIAVSKRKGTNAIEIAGNVLRRVETLKGNVIPSEVELSITRHY
                     GETAAEKSNELLFHMAIAVVSVSLLIGFTLGIRESLIVFVAIPVTLALTLTVFYLYGY
                     TLNRITLFALIFSIGILVDDAIVVVENIVRHARLPENHGKLLGEIAVKAVDEVGNPTI
                     LATLTVIAAILPMAFVGGLMGPYMRPIPIGASAAMVFSLIVAFLVTPWAAIRLLKPSA
                     SGAHETEDRATRLYRVFMGNVLHRPVLTWGFLGLVTILLLGSMSLVYIRFVQVKMLPF
                     DNKSEFQVIVDMPEGTTLEETADVTRRLAEKMLERPEVTDVQTYAGTASPYNFNGLVR
                     HYYLRAGANVADIQVNLLPKHERDLQSHDIAKLVRNDLQPIAARYGARIKVAEVPPGP
                     PVLQTLVAEIYGPSTEGQLRIARQIRDLFDQTEGVVDVDWYVEDDQQKNRIVIDERKA
                     ALNGITEDDIARTLRIASAGETAGLLHHANAKEDIPLRVRLDRATRSDIGRIQNLKVR
                     GSMGQLVPLRELVSIEPAIADKSIYHKNLMPVTYVTADVAGAMESPVYAILALGPKIG
                     GIKLPEGYSIEQHTASQPFDDSRYAMKWDGEWHITYEVFRDLGLAFAAVLVLIYVLVV
                     GWFQSFITPLIIMAAIPFSLVGILPAHGMMNAFFTATSMIGFIAGAGIVVRNSIILVD
                     FVELRLKEGMPL"
BASE COUNT         1961 a         3121 c         3086 g         1963 t
ORIGIN      
        1 caaaacccgt ccggtctcga atatgtccag gcgttaggat cgttggccga aacgctcgtc
       61 agccagaatc gtttggagga agccgagaag tcagtggccc ggatgcgtga agcgggagcc
      121 gccgatacac gcaatgggaa attttacgag gtgatggcgg tacaggtatt ggcacgcatc
      181 cgggacctgc aaaagaagcc ggaggaagca cgccagctac gggcgagtat gggcggccgc
      241 gatcccatgg cagcaaaccg ggagaagacc gtttacgaag caaccgcttc cgctcagcag
      301 acggcccttc agggcgacat cgacgcggcg gttgcgacgg ccggcgaagc ccttgctctc
      361 gccgcggaac gcagccggtc caatccgcag gaggtctccg ggctcatgag cctggccaat
      421 gtgctgatgg acaaacaaaa gaaggaagaa gcccgccgga tcatgatcga aacgctccgg
      481 attctggacc gggttccgga tcatccccgc gtcgcggatg ctttgggctc tgtaacctcc
      541 gtgctgttcc ggctgggaat ggcggcagag gccgaacgcg cggccgagcg gcaggagaag
      601 atcctcctcg aggcgaaagg cgctgaaagc ctggtgctcc actcgatcag ccacgcgcgc
      661 atcgagcgga tgcagcgcga tggcgactgg atgggagtga tggaggaacg caagcgcatg
      721 ctcgcccgcg tggaaaaaat gaccgggaca aagagccgcg aaagcctgtc tgcgctgcgc
      781 gaggtggcat gggcttaccc gcagctcaat aactggcccg aagaagaacg cgttctctcg
      841 gccctgctgg agcgcaccgc aaacctttcc ggccgcgcga gtgtggacca cgcccatctt
      901 ctggtgcaga tggccaatcg cgccggtcac aatcgccagt tcgagaaggc gctgaactgg
      961 atagaccagg caattgaggt tgcccgggcc ctgccggatg ccgatgtgca cctgccgggc
     1021 ataatgcaga accgggcgca gatcgcacag gcgaaagagg cgcctcgcgg agggccgttc
     1081 tatgcgccgg ccggagcggc gagtggcgca ggccgctggt ttgataccga tcgtttccgg
     1141 caatccggtc ccacagtacc caacaaataa tcggaagaat ttcacggccg ggcagtctgg
     1201 tgtaccattg aaattacttg catgaaggct ttactggaga tcctccagcc gggggaaccc
     1261 gactgggatc tggcccaagc gattgacccg gatcgacttc ctgctcacat cgcaatcatc
     1321 atggacggga acggccgttg ggcgaatcgt cgaagcatgc cccgggtggc cggacaccgc
     1381 gcgggcatcg ggccggtccg tgcgacggtg gagacgtgtg cgcgtctcgg catcgaggcg
     1441 cttaccctct acgcgttctc catcgagaac tggaagcgcc cgcgccagga agtggacacc
     1501 ttatggcgcc tgctgaggca ttacctcggt aaggagatgg cttcgctgat gcgtaatggc
     1561 atccgcctca ccgccatcgg gcgtctcgat tctcttcctc ccgatgtgcg ccgcgatctc
     1621 gatcaagcgg tgcggcagac ggaagccaac aacggattgc gggtgaatct cgcgatcaac
     1681 tatggcggcc gcgccgaact ggtcgacgcg ctcaatgcgc tcctggagga agcccgtctc
     1741 gagggacggc tttccacgct tcaaatcgat gaggaagcaa ttgaggcgca tctttacacc
     1801 gcgggccagc cgcatcccga cctgctgatc cgcacatcgg gcgagatgcg gatctccaat
     1861 tttctcctct ggcagatcgc ctattccgaa ctgtacgtca cggatgtttt ctggccggac
     1921 ttttcccgtt ccgatcttct tcgtgccatc tgcgaatacc agaagcggga ccgccgcttc
     1981 ggagggctca gcccgcgagg gcacggccgc tcttcgcgcc agccagtggc agaagaaatc
     2041 ggcgtcccgg cgagatgaaa cgtgtcctaa cggcgcttgc gctcattcca tttatcctgt
     2101 tcgttatttt ttggggtccc tcctggctgt tcctggccgt ggtcgtcaca gtagccgccc
     2161 tttgcttcta cgagtattcg gggatcgtcg aaggacacgg catcgcgcgc cccggtccgg
     2221 ccggatatgc cgcaggcttg ttgttcctgc tggtgcagcg aaacgaagcc ttgttgatcg
     2281 tcctggtcgc gctgctgatc gcggcgctgt ccctccgcaa gaccgagctg tcgaaatgcc
     2341 tgccctatgc agccgccctg atgttcggcg tgctctacgc gtttggaccg tggcgctccg
     2401 cattggcgct gcggagcgtg gatccgctct ggctgtttct cccgttgctg gtgagttgga
     2461 ccggcgatac cggcgcttat tatgtgggcc gggtgatagg ccggcaccgt ttggcaccgc
     2521 gcatcagtcc cgcgaaatcc tgggagggcg ctgtggcttc ggtgatcacg gcggcggccg
     2581 tgggaacgct ctactgtgcg tatctgctcc cctcggtacc cctgatagaa gcggtcggac
     2641 tgaccgccgt aaccaacgtg gcagggcaaa tcggcgatct cgcggaatcc gccatgaaac
     2701 ggggcgccgg cgtgaaagac agcgggacaa ccctgcccgg acacggtgga tggctcgacc
     2761 gtgtagatag cgctctgttc gcgattccag tcgtgcactt ctacctgtgg cgcggccagt
     2821 tctgagtccg gttatttaca gcagcgtgtc atccgcacgc agaatccacg ccgaggggtg
     2881 tggtttgaag ccgattcgcg gataatactc caccgctttg ggcgccgaaa gcaacacgat
     2941 atacgcttcg gggccgcttt cacggcgcgt gatccggatc aactccctgc cgacgccctg
     3001 cctctggtgc gacaagcgga cggcgagatc ggacagatac gtaacgtagg cgaaatccga
     3061 caggctgcgt gcaatcccca ccatcaggtc tccgtcccat gcggtaacgg tcaggttggc
     3121 cgtccggagc atcgtgcgca tgcgctcgcg gtcttccacg ggccggcgtt cgcccagcgt
     3181 ggatgccttg tacagttcga tcacctggtc caggtccaga tcgtttccca cgcggtattc
     3241 gatcacaggc cggaggctcc ctcgcgcgcg gaaagcattt tcctgtacgc tccgagcaat
     3301 tcgcgccgga cttcctcgcg aatcggccgc ggcacctcac ccgaactgta ttcgtggcag
     3361 agatcgatgc tgcgcttcag gctctcgaca aactgaacgc aaggcggaca gccatggatg
     3421 tgctgccgta tttcctcgca ggtgctctcg ggcagctccg catccagata ctgcgacagc
     3481 atcgaaaaga tctccttgca agtgtgctcc atcccgttat cccctggcag attgcaggta
     3541 cccgtccagt ttctttctaa cggccagacg ggcgcgatgc aatctcgtct ttactacatc
     3601 cgtgcccacc tccagaactt gcgccgcttc ttccgtcgtg agctcttcca catcgcggag
     3661 cagaatcacg gagcggtaga tctcgggaag ctctccgatg gcccgctcca gcacggcccg
     3721 caactcggag ttgagcgcct gggtttcggg caacgcagac caatcggcga gctgaagctt
     3781 cctttccact ccttcgccat cgccggccgg aatgagttcc tccagtgaaa gctcccgttc
     3841 cggagcgaac acgcttttgc gccgcttcat gaggcaggca ttcttggcaa tccggaaaac
     3901 ccatgcccgg atattctcgg gctcctttaa ctgatccagt ttttcgaaca ccttcaacag
     3961 cgtctcctgg gagacctctt cggcatcctc tctctggccg cacataagat agctgtactg
     4021 aaagatcttt gtccggaacc gctccaggaa gcggtcgaat gcctcttcct tgcccgacaa
     4081 caggtcgcgc gcgagatcta catcgtcgtc tgtctgatgc actcaccgcc attctaaccc
     4141 gctataatcg gacaaattta taactgctga actaccgttc ccagcgggaa cccgatggca
     4201 atatcgatct gggacggatg cgagagggta ttgtgaatca aacatagttt cgtagcacgc
     4261 aggaggccct ccctatgcct ttcgccatcg actcccggca ccgtaacctc gatcacaaac
     4321 gaatcgagac gcaccggctt cccgtctttg gcggcgcgca cccgaacttc caacccttcc
     4381 ataggcagat tccgcgcttt cagatattgc gccgcataat agcccgcaca ggttccgagc
     4441 gagacgagca gaaattccgg cgggctcatc ccctcatcgg ctccgccatt gccggacggc
     4501 tggtcgcaaa taacgctatg cccgcgcgtt tcggcctgga actgcacacc gttccggtat
     4561 cgaaccgtaa catccataag aaatatgtct ccactcacga agatgcagtc tggtggccgg
     4621 ggtgacagtg tcactttcgg aaagtctctg catctttgtg tctgaagcac ggccatggat
     4681 tgaaatgaga ccctttactc tttttgcgct gctttgttgt acgataccgc tgcccgctca
     4741 ggactccctc tcattacagg aggccgtgga aacggctctt ggccggcatc ccgccataga
     4801 ggcgagcgcc tccaataccc gcgccgcgga cgcgcgcatc cggcaggccc gaagcggata
     4861 tctgcccaaa ctcgactact cggaagcgta tcagcgcagt aacaacccgg tatacgtatt
     4921 cagctcgctg ctgacccagc aacagtttgc cgtagagaac ttcgagattg gcaggctcaa
     4981 ccagccggat gcgctgaaca acttccaatc gttattgaca gccgatcaga tcctctacga
     5041 tgccggccgg acgaagaagc aagtcgaggc ggccgaaatc gggcgcaatc tcgcctcaga
     5101 ggatgagcgc ggagcacgga tgtccgtcat cctgggcgtc gttcgagctt attatggcgc
     5161 ggtgctcgca aacgaaggcc tccaggtggc gcgcgaagcc gtgcgttcgg ccgaagcaga
     5221 cctggaacgc gcggaagcgg tgcggcaagc cggcatgtcc acggacgccg atgtgctctc
     5281 catccgcgtg cacctggccg cgatgcggga acaggagatc cggcgggttc atgagctgga
     5341 cgtcgcccgc gccgctctca acgaggcgct tgggttgccc cttgaaacgc ctcaccagtt
     5401 gaccactccc cttgcggcgg cttctgtcgc gtatgctccc gaactggaaa agagtgcgct
     5461 ttccgaaagg ccggaagccc gccaggccga tcttcgctcc cgcctcgccc ggacgcaggc
     5521 cgacggggcg cgcgcggctt actttccgca ggtatttgcg cgaggggtct tcgaagccga
     5581 tcgccagcgg tttgtcacgc gtggcggtgc gaattggatg tttgcggcgg gattgcgctg
     5641 gaacctgttc aatggtttcg ccgaccgcgc ccgcatcgaa gaggccggac acgcggccgc
     5701 ggccgcgcag tcccagcagg aacagaccag ccgtgccgtc cagctacagg ttcgcggcgc
     5761 gctgtccaac ctttccgctg cggaagagcg gatcaaagtg gccgaggcgg cggtcggcat
     5821 ggccgaagag agtttgagga ttacgaaaaa caggttcgac aacggtctct ccacggtgac
     5881 ggatctgctt cgcaacgaga cggcgctcct tgaagtgcgg atgcgccgcc tgtcggcgat
     5941 tcacgaccag cgcgtcgcgg cggctgccgt agagagggcc gcgggcactc tttccaccgc
     6001 ctcgaacgta ttgagatgac ccggacaagg attcccatga caagaatgat cccgattgca
     6061 gttctgttat tgagcttggc cgcctgcggc catgacagcg gaaagaagtc ccaaaccccg
     6121 cccgccgcgc ctgtcacggt caaagttctc accgtgaccg ggacggattt gccagccgtg
     6181 tacgaagcgg tgggaaccgt gaaagcacgt accgcggcca cgatttcgag ccgggtaatg
     6241 ggctatgtgc gcgacgtccg tgtcgcggtt ggggatcgtg tccgggccgg gcaactgctg
     6301 gtgaccatcg actcccggga gctcgaagcg gtctacggcc aggcggaggc ggcccgggcc
     6361 gaagcacaga gcgccattgc cgaggtggaa aactccgttt cggccgccaa ggcgaacctg
     6421 gagctttccc aggtcacttt caatcgcatg aaagacctgt ttgagaagcg gtcgatctcc
     6481 aaccaggaat acgacgaggc ggcagcccgg ctggaagtgg cgaaggccaa ccttcagatg
     6541 gcacaatcca agcgcaccca gctcgccgcg aaggtgcggc aggccgatgc cgcggtccgc
     6601 tccgccggga tcgtgagagg atacgcacag atcaccgcgc ccttcgcggg cgttgtcacg
     6661 gagaaacacg tggaccccgg caacctcgcc tctcccggcg ctccgctgct gacggtcgaa
     6721 caggccggcg cctaccggct ggaggttccg gtggaggagt cgcggctcgg atcgatacaa
     6781 tccggccggc cggtcgaagt cgttctggat gcgctgaacc agcggctcca ggcgcgggta
     6841 agcgagattg ttccggcgat tgacgccgcc tccagaacat ttctggtgaa gatcgatctg
     6901 ccacgcatag ctcaccttgg ctccggcctg tatggccggg ctgtctttca gagcggagtc
     6961 aggcggagca tcgtcgtgcc ggccgatgcc gtcatccaga gggggcaggt tctctccgtg
     7021 ctcgtcgccg acgacggcat cgcacggaca cggctggtga ccaccggacg cgagcgcgac
     7081 ggccgcatcg agatactcac ggggctcgtg cagggcgaaa agctcatcta tcctgtaccg
     7141 ccatccgtgc tggacggtgc ccgcgtggag gtgcagccgt gaactcctcc aaactcggcg
     7201 tcgccggcag gtttgcgcgc ctttggatgg aatccaagtt gacgccgctg ttcattgttg
     7261 cgtccgtcct cctcggcgct ttttcggttt ggaaacttcc gcgggaggaa gagccgcaga
     7321 tcattgtccc gatgatcgac atcttcgtcg gaatgcctgg cgcctccgcg aaagaagtgg
     7381 aagagcgcgt caccaggccg atggagaagc tgctgtggga aattcccggc gtcgaataca
     7441 tctactccac gtccagcccc ggcggtgcgc tggcgatcgt gcgtttcagg gtgggcgagg
     7501 acgaagagaa gagcatcgtc atgctcaacc agaagatgta ttccaacttc gaccggattc
     7561 cgcccggcgc cacccagccg ctcatcaagc cgcgatccat cgacgatgtg cccattctcg
     7621 cgctgacgct ctggagccgc cgctacagcg gcttcgagct ccggcaggtc gccgcccagg
     7681 tgcacgacgc catcagccag gtgaacgatg tgtccaccgt caatctcatc ggcggcgagc
     7741 gccgcgagat tcgcgtggtg ttggatgagg cgaagctatc cgcctattcg atgacgccgg
     7801 ttcagattat agcggcgctg ggagcttcca atcgccgtct cgaatccggg cggtactcca
     7861 cgggaaaccg agaattgctg ctcgagaccg gcgagtttct tcaaacagcc gaagacgtcc
     7921 ggaacgtggt tgcaggcgtg gcgaatcaaa ggccggtctt cctgcgcgat gtcgccgagg
     7981 ttatcgatgg cggcgaagag ccatcgcaat atgttcatta cgcctcaaac agcgcctttc
     8041 atcccgcggt aaccatcgcc gtctccaaac gaaaaggcac gaatgccatc gagatcgcag
     8101 gcaacgtact gcgccgcgtc gaaacgctga agggaaacgt catcccgtcg gaagtggagc
     8161 tcagcatcac gcgccactac ggcgaaaccg cggccgaaaa atcgaatgag cttctgttcc
     8221 acatggccat cgcggtggtg tcggtcagcc tgctcatcgg gttcacgctg ggtattcgcg
     8281 aatcgctcat cgtctttgta gccatccccg tcacgctggc gttgacactg acggtctttt
     8341 atctctacgg ctacactctc aaccgcatta cgctcttcgc gctcatcttt tccatcggta
     8401 ttctggtcga cgacgcgatc gtcgtggtgg agaacatcgt acgccacgcg cgcttacccg
     8461 aaaaccatgg caagttgctc ggcgagatcg ccgtgaaggc ggtggatgaa gtcgggaatc
     8521 cgacgattct ggctacgctg acggtgatcg ctgcaatcct ccccatggcg ttcgtgggcg
     8581 ggttgatggg gccttatatg cggccgatcc cgatcggcgc ctccgcggca atggtgtttt
     8641 ccctgatcgt tgcttttctg gtgacgccct gggccgctat ccggttgtta aagccgtcgg
     8701 ctagcggagc acacgagacc gaagaccgcg cgacgcggct ctaccgtgtg ttcatgggga
     8761 atgtgttgca ccggcccgtc ctcacctggg gctttctggg actggtgacc attctgctgc
     8821 tcggttcgat gtccctcgtc tacatccggt ttgtccaagt gaagatgctg ccgtttgaca
     8881 acaagagcga gttccaggtg atcgttgaca tgccggaagg caccacgctc gaagagacgg
     8941 cggacgtgac gcgccggctt gccgaaaaga tgctggagcg gccggaggtg accgacgttc
     9001 aaacctatgc cgggactgcg tcaccttaca acttcaacgg cctcgtacgc cattattatc
     9061 tgcgggccgg cgcaaatgtc gccgacatcc aggtgaacct gttgccgaag cacgagcgcg
     9121 acctgcaaag ccacgatatc gccaaactcg ttcgcaatga tctgcaaccg attgccgcgc
     9181 gctacggagc acgcatcaag gtggccgaag tgcctcccgg accgcccgtc ttacaaacgc
     9241 tagtggccga gatctacggt ccttcgaccg agggtcaatt acgtatcgcg cgccagatcc
     9301 gggacctttt cgaccagacc gagggcgtgg tggatgtcga ttggtacgtg gaggacgacc
     9361 agcagaaaaa ccgcatcgtc atcgacgagc ggaaagccgc gctcaacggc attacggaag
     9421 acgatatcgc ccgtacgctg cgcatcgctt ccgccggcga aacagccggg ctgctgcatc
     9481 atgcgaacgc gaaggaagac attccgctgc gtgtgcggct cgaccgcgcc acgcgctccg
     9541 atatcggccg cattcagaac ctcaaggtgc gcggcagcat gggccaactg gtgccgctcc
     9601 gggaattggt aagcattgag cccgccatcg cggataagag catttaccac aagaacctga
     9661 tgccggtcac ctacgtgacc gccgatgtgg cgggcgcgat ggagagcccg gtgtacgcga
     9721 ttctcgcgct cggtccgaag atcggcggca tcaagctgcc ggaaggttat tcgatcgaac
     9781 agcacacggc cagccagccg tttgacgatt cccggtacgc gatgaaatgg gatggcgaat
     9841 ggcacatcac ctacgaagtt ttcagggacc tggggctggc gttcgccgct gtgctggttc
     9901 taatctatgt cctggtggtg ggctggttcc aatcgttcat aacgccgctg atcattatgg
     9961 cggccattcc gttttcgctg gtgggcattc tgcccgcgca cgggatgatg aacgcctttt
    10021 tcacggccac ttccatgatc ggcttcatcg ccggcgccgg aatcgtggtg aggaactcca
    10081 tcatcctcgt cgatttcgtt gaactgcggc tgaaagaagg catgccgctc g
//
LOCUS       DASTMK010000318         6378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_175518, whole genome shotgun sequence.
ACCESSION   DASTMK010000318 DASTMK010000000
VERSION     DASTMK010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175518"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1392)
                     /locus_tag="VFQ79_13840"
     CDS             complement(265..1392)
                     /locus_tag="VFQ79_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEU0140798.1"
                     /translation="MPCLTRLRGIVLFGLAASIVSGQNSANKSTDEVVIRGPQFHSPR
                     FSDDFEDFASTRIARLRDEYRLDEVVRGETSEFKKLLKLRHWVHSRWPIDNDQNFSGD
                     AFEILAKAKTGAGFHCSHSMRVQHAVMTVMGYVVRDLGVDSDHLVFGKSNHHGVNEVW
                     SNEYAKWVLLDAKYDIHFERAGIPLSALELHEAARADQGKGIVKVQGPDRREVPMKGL
                     EFPTSSVLGYWWISYNPGLKTFTGKNDSRGVVVLDSPEFRATRWFRDTDRGLGDHWAY
                     RANAFVPVSDRHQINWSVGVPDVKIRQTGPGEVDVTFFSITPNLKTYRARIDHGSWQD
                     VAGDRWHWKLKPGENVLDIRTRNRFGVDGPVVTAVVTHRAN"
     gene            1543..4305
                     /locus_tag="VFQ79_13845"
     CDS             1543..4305
                     /locus_tag="VFQ79_13845"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HEU0140799.1"
                     /translation="MRRLRAFWNSMFDVRQGELLRTVYMSLYLLCVLFAYYIIKPVSK
                     ALFVHRFDIDEFPWLYILIALFGGIFAYVYTKIAIRTSLATAVNWATGAFVGSLVLLW
                     WLLGLNKDWMLYVFNIYTSLFGIVLVSQGWLVAANVFHPREAKRLYGILGMGAVLGAA
                     FGGSFTAMAVEWVGTNNLVLASAVMVLLAYVAYRLAISQSGVSLAGAKAAGDEEADFS
                     VTEVTVAMRRYRHLQVIMGIIAITFIVDVMIEYQFNVMAKLTFTDKENLTAFFGSFYG
                     IYLNLATFVFQLFLTAAVVRHFGVGGALQIMPVTLGIASVFTFSFPHLFSASAARLGE
                     SASRYTFNRTGMELLFLPLPTELKNRTKAFLDIFVDRLSRGVGGIILLVLVGPLALDT
                     RYIALIVLGLCVVWVLLSLRAKNEYVATVRRRLEARRFDLENARITVTDASTLRLLEE
                     TLNSGNPRQVTYALSLLETVPNYKLDDTLVRLVDNPSPEVRSKVFELAQSAGFTGLLE
                     NALAEIRSARESRRPSAVAPAVSYALAVSPEAPGLARRLLDHPNYTVTESTLRAVAPH
                     SELALDLIGYEWLHEAAADPQPGRRVLAAVGVAARGDQGIEVLHSLLEDRDKQVASAA
                     LKAAGSLQNRAYLEGIVRRLGDPLLRGEAIAALAQFGNRILGTLSDLLHDETISLVVR
                     RQIPRVLQHIGGQRSVDILLHSIAQPDLTIRGAVLRNLHRLRETSPHLKFGEESITAQ
                     VLSEARHYFELNAALAPFKDQKNPRTAAGLLAATLEERLRYTIERLFHLLGLRYPPKE
                     IYAAYRAYRNNGPGSRAEDFSNAIEFLDNILDRELRRIILPLLDASDRLERHGEELFG
                     LEHLDAEHAIRELIRSGDPWLVSCAMATAAERGLRALTPEIREACRSASPEVVEVARS
                     AVAVLA"
     gene            4330..4752
                     /locus_tag="VFQ79_13850"
     CDS             4330..4752
                     /locus_tag="VFQ79_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU0140800.1"
                     /translation="MSELNIVEKVIALEAVDLLKNLNPDQLSRIATIAKETKYPPEKV
                     VIEADQPLDALYVVLDGAVQINRNGESLHVARQNEVLGSWALFDPEPMHVEAKTLEDT
                     KLLRIAREDFYDLLSDNMEITASIFSTLVKRFRQLVEQ"
     gene            4778..5620
                     /locus_tag="VFQ79_13855"
     CDS             4778..5620
                     /locus_tag="VFQ79_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0140801.1"
                     /translation="MNDQPKPAAESSRQDLLEQLESAHQRLAEAHKMASIGRLSAGIV
                     HEINTPIGSIFSNNEVILRSLEVLKKLLAESCAASVAPSEKAMEIIETMATLAAVDKI
                     ACERISGVIRSLKTFSRVDDRDIRKACLEGIIRNTLKLTGTVFRRRIAVETHFEELPE
                     IECYPGLLNQVFANLLVNAGQAIEGEGKIAVTARRENDFVHVSIADTGKGIKPEDRER
                     IFTRGFTTKPIGEGTGLGLAITREIVEDTHGGSIDFESEPGVGTTFHVRLPITQTRKN
                     GPAS"
     gene            5640..6119
                     /locus_tag="VFQ79_13860"
     CDS             5640..6119
                     /locus_tag="VFQ79_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0140802.1"
                     /translation="MDKNEQPAVMIVDDEEMVITSIRAFLRLETEYAVHGFTSPEDAA
                     RQLETNPVDVIVTDYLMPKMNGIQLLGKAKNLQPEAARVLLTGHADKQSAIQAINEIG
                     LYQYLEKPWDNAQLLLVIQGAIERTRLLRTLRDKISELDAAHSNLKDVQTRLLRAFL"
     gene            6122..>6378
                     /locus_tag="VFQ79_13865"
     CDS             6122..>6378
                     /locus_tag="VFQ79_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140803.1"
                     /translation="MAARCLPLLLFAVVPLFASDWRYYGGDSGGTKYSSEKQINRTNV
                     SRLKVAWTYHTGDMSDGTTYVSRSAFEATPLVVDGVMYFTTP"
BASE COUNT         1280 a         1992 c         1786 g         1320 t
ORIGIN      
        1 tggctcccag gatctggctg taactgacag cggtagcacc aagcgtggtg cggccaaacg
       61 cggtaaaaag ttctcgacga gtgctttgag tagccaaacc ttcctcccga atttcaacaa
      121 aatgattgtc ggaaatgaat aagctacgtt tccagtcgtg caggaggcat cttggatgcc
      181 aaacagaaac tacggtggaa ggcttcgaaa aaccggacga tcgactggtc cgattcctgg
      241 tccggcgaag acctcaatga atgatcaatt cgcgcggtgc gtgacaaccg ccgtaacaac
      301 aggaccgtcc actccgaaac gatttcgtgt gcgaatgtct aacacgtttt caccgggctt
      361 gagtttccaa tgccaccgat cgccggcaac gtcctgccat gatccgtgat cgatccgcgc
      421 gcggtaggtc ttcaggttag gagtgatgct gaagaaagtt acatcaactt cgcccggccc
      481 cgtctgtctg attttcacat ccggcacgcc gaccgaccag ttgatctggt gacggtcact
      541 caccggcacg aacgcgttcg cgcggtatgc ccagtgatcg cccagcccgc gatccgtatc
      601 tcgaaaccat cttgtcgccc gaaattcggg actgtccaag acaacgacac cgcggctgtc
      661 attcttgccc gtaaacgtct tcaacccggg attgtatgag atccaccaat aaccgagcac
      721 gctgcttgta gggaattcca gtcccttcat cggcacctca cggcggtccg gcccctgtac
      781 cttgacgatg cccttcccct ggtcagcccg tgcggcttca tggagttcca aagcggaaag
      841 cggtatgcct gcgcgctcga agtgaatgtc gtacttcgcg tcgagcagca cccacttggc
      901 atactcgttc gaccagacct cattcacgcc atgatggttg cttttgccga acaccagatg
      961 gtcactgtcc acgccgagat cacgaaccac gtagcccatc acggtcatca ccgcgtgctg
     1021 cacgcgcatc gagtgggagc agtggaaccc ggcgccggtc ttggccttgg caaggatctc
     1081 aaacgcgtcc ccggagaagt tctggtcatt gtcgatcggc cagcggctgt ggacccaatg
     1141 cctcagtttg agcagctttt taaactcgct ggtttcgccg cggacaacct cgtcgagtcg
     1201 atactcgtct cgcaaccgcg ctatgcgggt gcttgcgaag tcctcgaagt cgtctgaaaa
     1261 acgtggacta tgaaactgcg gcccgcgaat caccacctca tccgtgctct tgtttgccga
     1321 gttttgcccg gagacaatcg atgccgccag cccgaacaat acaattcccc tgaggcgagt
     1381 aagacatggc atggttctat tctgaccgac tgcagcggcc ccgtccagca atcacagacc
     1441 ttccggccgg ctcacgaccc gcctgaatcc cctcccccat catcgcggta agatgaatcc
     1501 aatgaccccc cggggcctct tggcgccgcc cgcccaactc ctatgagacg ccttcgcgct
     1561 ttctggaaca gcatgttcga cgtccggcag ggagaacttc tccgcaccgt ctacatgtcg
     1621 ctgtacctgc tgtgcgtgct gttcgcctac tacatcatca agccggtttc gaaggcgctg
     1681 ttcgttcaca ggttcgatat cgacgagttt ccctggctgt acatcctcat cgccctgttc
     1741 ggcggcatct tcgcctacgt gtacaccaag atcgccatcc gcacttcgct cgccactgcg
     1801 gtgaactggg ccaccggggc tttcgtcggc agcctggtgc tcctgtggtg gctgctcggg
     1861 ctgaacaagg attggatgtt gtatgtcttc aacatctata ccagcctgtt tggaatcgtc
     1921 ctcgtctcgc aaggctggct ggtcgccgcc aatgtctttc atccccgcga agccaaacgc
     1981 ctctatggca ttctcggcat gggcgccgtt ctcggagccg ctttcggagg ctccttcacc
     2041 gcgatggcgg tggaatgggt tggcacgaac aacctggtgc ttgccagcgc ggtcatggtg
     2101 ctgctggcct atgtggccta ccggctcgcg atctcgcaga gcggtgtatc gctggccgga
     2161 gctaaagcgg ctggagacga agaggccgac ttttcggtaa ccgaagttac cgtagccatg
     2221 aggcgatacc ggcacctgca ggtcatcatg ggcatcatcg ccatcacctt cattgtcgac
     2281 gtgatgatcg agtaccagtt caacgtgatg gccaagctga cctttaccga caaggaaaac
     2341 ctcaccgcct tcttcggcag cttttacggc atttacctga acctggcgac gttcgtcttt
     2401 caactgtttc tcaccgctgc cgtggtcagg cactttggcg ttgggggcgc cttgcagatc
     2461 atgcccgtta cactgggcat cgcctccgtc ttcacgttct ccttccccca cctgttctcc
     2521 gcctccgccg cccgcctggg ggaatccgcg tcccgctaca cgttcaacag aacaggcatg
     2581 gagttgctct tcctgcctct gcccacggag ctgaagaatc gcaccaaggc tttcctcgat
     2641 atattcgttg accgcctctc gcgcggcgta ggcggcatca ttctgctcgt gctggttggc
     2701 ccgctcgcac tcgatacccg ctacatcgcg ctcatcgtgc tgggcctctg cgttgtttgg
     2761 gtcctgcttt ctctgcgggc gaagaacgaa tacgttgcca ccgtgcgcag gcggctggag
     2821 gctaggcgtt tcgacctcga gaacgcgcgg atcaccgtta ccgacgcctc cacccttcgt
     2881 ctcctcgaag aaacgctgaa cagcgggaac ccccgccagg tgacttacgc gttgtctctc
     2941 ctggagaccg ttccaaacta caagctcgat gacacgctgg tgcggctggt ggacaacccg
     3001 tcgccggagg tccgcagcaa agtattcgaa cttgcgcagt cggccggctt tacaggactg
     3061 cttgaaaacg ctctggcgga gatccggtcg gcccgggaaa gccgccgtcc gtccgccgtc
     3121 gcccccgcgg tttcttatgc gctcgccgtc tcacccgagg cgcccggtct ggccagacgg
     3181 ctcctggatc atccgaacta tacagtgacg gaaagcacgc ttcgggccgt cgctccccat
     3241 tccgagctcg cgctcgacct gatcggttac gaatggctgc atgaggcggc ggccgacccg
     3301 caacccgggc gccgcgtatt agccgccgtc ggtgtcgccg cccgcgggga tcagggcatc
     3361 gaagtattgc actcgctcct cgaagaccgg gacaagcagg tggcctccgc cgctctgaag
     3421 gcggccgggt ctttgcagaa ccgcgcgtat ctcgaaggca ttgtccgccg cctcggcgat
     3481 cctctcctcc gtggcgaggc catcgccgcg ctggcgcaat tcggaaacag gattcttggc
     3541 actctcagcg atcttctgca cgacgaaacc atctcgctcg tcgttcgtcg ccagatcccg
     3601 cgagtgctgc aacacatcgg aggccagcgt tccgtcgata ttctgctgca ctccatcgcc
     3661 cagcccgatc tcaccatccg tggcgcggtg ttgcggaacc ttcatcgctt gcgggagacc
     3721 tcgccgcatc tgaaattcgg cgaggagtcc atcaccgccc aggtcctcag cgaggcacgg
     3781 cactatttcg agctcaacgc cgcgctcgct cccttcaagg accagaaaaa tccgcgcacg
     3841 gccgccgggc tgctcgccgc cactctggag gagaggctcc gctataccat cgagcggctc
     3901 tttcacctgc tggggctccg ctatcccccg aaagaaattt atgccgctta cagggcctac
     3961 aggaataacg gcccgggctc ccgggcggag gacttttcca acgcgatcga atttctggac
     4021 aacatcctcg accgcgagtt gcggcgtatt attttgccgc tgctcgacgc ctcggaccgg
     4081 ctcgaacggc atggagagga gttgtttggg ttggagcacc tggatgcgga gcacgccatc
     4141 cgcgagttga ttcgctccgg agatccgtgg ctggtatcct gtgccatggc caccgcggcg
     4201 gagcgggggc ttcgcgcctt gaccccggag atccgggagg cgtgccggag cgccagtccg
     4261 gaggtggtcg aagtcgctcg ttcggcggtc gccgtgcttg cgtagcgctc cgtccggggg
     4321 acgatctaca tgtccgaact caatatcgtt gaaaaggtca tcgcgctcga agctgtggat
     4381 cttttgaaga atcttaatcc cgatcagcta tcgcgcatcg ccactattgc gaaagaaacg
     4441 aagtacccgc ccgaaaaggt cgtaatcgaa gcggaccagc cgctcgacgc gctgtacgtc
     4501 gtgctggacg gcgccgttca gatcaaccgc aacggcgaat cgcttcacgt cgcgcgccag
     4561 aatgaagttc tgggctcgtg ggcgctgttc gaccccgagc cgatgcacgt agaagcgaaa
     4621 actctcgaag atacgaagct gttgcgtatc gcccgggaag atttctacga tctgctatcc
     4681 gacaacatgg aaatcacggc ttccattttt tccaccctgg tgaagcggtt ccggcagctc
     4741 gttgagcaat aattaaaagg gacggccctc acgaaagatg aacgatcagc cgaagccggc
     4801 ggcggagtca agccggcagg atctgttgga acaactcgaa tcggcccatc aaaggctggc
     4861 tgaagcgcac aaaatggctt ctatcggaag attgtccgcc gggattgtgc atgagatcaa
     4921 cacgcccatc ggttccatct tttcgaacaa tgaagtcatc ctccggtcgc tggaggtgtt
     4981 gaaaaagctg cttgcggaat cctgcgccgc ttcggtcgcg ccatcggaaa aagcgatgga
     5041 gatcatcgaa acgatggcaa cccttgccgc cgtcgacaag atcgcctgcg aacggatctc
     5101 gggtgtcatc cgcagcctga agaccttttc ccgcgtggac gaccgtgata tcaggaaggc
     5161 gtgtctcgaa ggaatcatcc gcaatacgct caagctgacc ggtaccgtct tccgccggcg
     5221 catcgccgtg gaaacgcatt tcgaggaact gcccgaaatc gagtgttacc ctggtctgct
     5281 gaaccaggtg tttgcaaacc ttctggtgaa cgccggccag gccatcgagg gcgaaggaaa
     5341 gatcgccgta accgcccgcc gcgaaaatga tttcgtgcat gtttcgatcg ccgacaccgg
     5401 caagggcatc aagcccgagg accgcgagcg gatcttcact cggggcttca ccacgaaacc
     5461 cattggagag ggcacaggat tggggcttgc catcaccagg gaaattgtcg aggacaccca
     5521 cggcggttcc atcgattttg aaagcgagcc cggcgtgggc acgacttttc atgttcgtct
     5581 cccgataacc cagacgagaa aaaacggccc cgcaagttga ttggaggaat gttcattccg
     5641 tggacaagaa cgaacagccg gcagtaatga ttgtggacga tgaggagatg gttatcacca
     5701 gtatccgtgc atttctgcgg ctggaaacgg aatacgccgt ccacggcttc accagcccgg
     5761 aggacgccgc caggcaattg gaaacgaatc ccgtcgatgt catcgtcacg gactacctga
     5821 tgccgaaaat gaatggcatc cagttgctcg gaaaagccaa aaaccttcaa ccggaggcgg
     5881 ctcgcgttct gctgaccgga cacgccgaca aacagagcgc catccaggcc atcaacgaaa
     5941 tcggcctcta tcagtacctc gagaagccct gggataatgc gcagctttta ttggtgatcc
     6001 agggcgccat cgagcgtacc cggctgttgc gcaccttgcg cgataagatc tcggaactgg
     6061 acgccgcgca ctcgaatctc aaggacgtgc agacccgtct cctgcgggca ttcctgtagc
     6121 gatggctgcc cggtgtctcc cgctgctgct gttcgcggtt gtacccttat tcgcctccga
     6181 ttggcgctac tacggcggcg atagcggagg gacgaaatat tccagcgaaa agcagatcaa
     6241 tcgcacgaac gtttcgcgcc tgaaagtcgc ctggacctat cacacgggcg acatgagcga
     6301 cggaaccacc tatgtttccc ggtcggcatt cgaagcgacg cccctggtgg tcgatggcgt
     6361 catgtacttc accacgcc
//
LOCUS       DASTMK010000319         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_528172, whole genome shotgun sequence.
ACCESSION   DASTMK010000319 DASTMK010000000
VERSION     DASTMK010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528172"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFQ79_13870"
     CDS             <1..351
                     /locus_tag="VFQ79_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU0140804.1"
                     /translation="YAFFGFTAKYFGSRESTKLAKGWEPDQAIITEFHDYLLKQNVKF
                     TEAEFSGNLPWIKEQLKREFYLTAFGTEDARRYVVETDPMVIKAVEAIPQSKELLATA
                     KKMIVQRLSKQSNK"
     gene            348..2126
                     /gene="guaA"
                     /locus_tag="VFQ79_13875"
     CDS             348..2126
                     /gene="guaA"
                     /locus_tag="VFQ79_13875"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU0140805.1"
                     /translation="MITNTAAAPGTTPQVIVLDTGGQYCHLIARKIRELGVYAEIRPS
                     STPAAQLCGAKGLIISGGPCSVYDEGSPTVDPAIFSMGAAVLGICYGQQLMALSLGGR
                     VRKGEKGEYGLAFLEIAEDANPLFAGTPVRQQIWMSHRDAVEQVPQGFRIIAGTDTCE
                     AGAMVDTLGRLYGVQFHPEVVHTPAGRRILSNFVFDICGCRPDWEPKHQIPAIENRIR
                     EVAAGRSVFFFVSGGVDSTVAYTLCLRALGDARVHGAYVDTGLMREGETEYVKDVFAS
                     LGSHGFAVEDASAGFLNALSGVRDPERKRHIIGEQFVRVQEEILERGHFLDSQWILGQ
                     GTIYPDTIESGGSANADLIKTHHNRVPGISELIASNRLVEPLHLLYKDEVREIGRELG
                     LPGDLLDRHPFPGPGLAIRCLCSESSAQVERLGPGWLIPVRSVGVQGDCRTYMPVLSI
                     GDLDHDQARRLINEYPGINRVVAAVASHAEIREMKVRPSDLSAQRLARLRRADAIVRE
                     LSAESGFDRQVWQFPVVLIPLGTSGRPDSVVLRPIHSVDGMTAQSVTMDAELLQRMAS
                     ELLRVEGLCGVFYDLTHKPPATIEWE"
     gene            complement(2235..>2897)
                     /locus_tag="VFQ79_13880"
     CDS             complement(2235..>2897)
                     /locus_tag="VFQ79_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140806.1"
                     /translation="SAWRAAKLLGGTLAAVCRKEGVPGDEGADLDHFQNLLRCPDCHG
                     GLARTGDVLTCAACSYEAHNQQGVYNLLPSGERGELYPGERADIVDFSQPGHEKYLGS
                     GWHELEGVFGNKYRWVGESASLALRRVHSGAQRLRIRGHAHETSFRQGQPVRVEAIVN
                     GRRSGLWTLERTGLFVLEADVPDEAEYGIQLLCSPVWRPPNDERSCSVNFSMIRLIPR
                     ER"
BASE COUNT          550 a          860 c          898 g          589 t
ORIGIN      
        1 tacgcatttt tcgggtttac agcgaagtac ttcgggtccc gcgagtccac gaagctagcg
       61 aagggttggg agccggatca ggccatcatc accgaattcc acgattatct tctgaaacag
      121 aacgtgaagt ttacggaagc cgagttttcc gggaatctgc cttggatcaa ggaacagcta
      181 aagcgcgagt tttacctgac ggcttttggc accgaggacg cccggcgcta tgttgtagag
      241 acggatccta tggtcatcaa ggccgtggag gcgattccgc aatcgaagga actgctggca
      301 accgccaaga agatgattgt gcagagactg agtaagcaat cgaataagtg attacaaata
      361 cagccgccgc cccgggtaca actccgcagg tcattgttct ggatacgggc ggccagtatt
      421 gccatctgat cgcacgcaag attcgcgagt tgggtgtgta tgcggagatc cggccgagca
      481 gcacgccggc ggcccaattg tgcggggcca agggcctcat tatctctggc gggccgtgca
      541 gcgtctatga cgagggcagc ccaaccgtag acccggctat tttttccatg ggcgccgcgg
      601 ttctgggcat ctgttacgga cagcaactca tggcgctatc cctcggcggg cgggtgcgta
      661 aaggcgaaaa gggcgagtat ggcctggcct ttctggagat cgccgaggac gcaaatccgc
      721 tgtttgccgg cactccggtg cggcagcaga tctggatgag tcaccgggat gccgtagagc
      781 aggtgccgca agggttccgt attatcgcgg ggaccgatac gtgcgaggcc ggggcaatgg
      841 tagacaccct cggcagactg tacggagtcc aatttcatcc cgaagtggtt cacacacccg
      901 cgggccgccg cattctctcg aacttcgtgt tcgatatctg cggttgccgt cccgactggg
      961 aaccgaaaca ccagattcct gccatcgaga accgcatccg cgaggtggcc gcgggtcgaa
     1021 gcgttttctt ctttgtttca gggggcgtcg attccactgt cgcctatacg ctgtgcctgc
     1081 gggcgctggg cgatgcgagg gtccacggcg cttatgtcga tacgggcctg atgcgcgaag
     1141 gcgagacgga gtacgtcaaa gacgttttcg cttcgctcgg cagccacggt ttcgccgtag
     1201 aggatgcctc ggccggtttc ctgaatgccc tttccggggt tcgcgacccc gagcgcaagc
     1261 gtcacattat cggcgagcag tttgtccgtg tgcaggagga gattctcgaa cgcgggcact
     1321 ttctcgattc acaatggata ctcgggcagg gcacgattta ccccgacacc attgaatccg
     1381 gcggcagcgc caacgccgac ctgataaaaa cccatcacaa ccgcgtgcca ggcatctcgg
     1441 agctgatcgc atccaaccgg ttggtggaac cgctgcatct cctgtacaag gatgaggtac
     1501 gggagatcgg ccgcgagttg gggttgcccg gcgatcttct cgaccggcat ccctttccag
     1561 gccccggttt agccatccgg tgcctgtgtt cggaaagtag cgcgcaggtg gagcgcctgg
     1621 gtccgggatg gttgattccg gtgcgctcgg tcggcgtgca gggtgattgc cgcacctaca
     1681 tgccggtgct gtctatcggc gacctcgatc atgatcaggc gcggaggctg atcaacgaat
     1741 accccggcat caaccgggtt gttgccgccg tcgccagcca tgccgaaatc cgggaaatga
     1801 aggtgcgccc aagcgatctc agcgcacaaa ggcttgcccg tttgagaagg gccgatgcca
     1861 ttgtccgtga gctttcggcc gaatcgggat ttgaccgcca ggtatggcag tttccggttg
     1921 tgttgatccc ccttggaacc tccgggcgtc ccgattccgt ggtgcttcgc ccgatccatt
     1981 ccgtcgatgg tatgaccgcg cagtcggtga ccatggatgc ggagttgctt cagcggatgg
     2041 cgtccgagct tttgcgtgtg gaagggcttt gtggagtctt ttacgacttg acgcacaaac
     2101 cgcccgctac cattgagtgg gagtagattc gggccaaccg ctcacttaac gttcacggct
     2161 cggaagcgat tacggagccg cgcgcgttag cgagcggact tggcgaactg gcacggttat
     2221 ttcctgacgg tcccttagcg ctcgcgcggg atcagccgga tcatgctgaa attgaccgaa
     2281 cagctacgct cgtcgttagg gggacgccag acgggcgaac agagcagttg aatcccgtat
     2341 tccgcttcgt cgggtacgtc cgcttccaac acgaacaggc cggtgcgttc cagagtccac
     2401 aaaccggatc tccttccgtt cacgattgct tcaacgcgca cgggctgacc ctggcggaaa
     2461 gaggtctcgt gcgcatgccc gcggatgcgc aggcgctgcg ccccggagtg gacgcgccgc
     2521 agcgcaagcg aggcgctttc tcccacccac ctgtacttgt tgccgaacac cccttccagc
     2581 tcatgccagc ctgagccgag gtacttctcg tgtcccggtt gggagaaatc cacgatatcg
     2641 gcgcgctcgc ccgggtacaa ttcaccgcgc tcgccggacg gcagcaggtt gtaaacgccc
     2701 tgctggttgt gggcttcata actacacgcc gcacaggtga gcacgtcgcc ggttctcgcc
     2761 agcccgccat ggcagtccgg acagcgcagc aggttctgaa aatgatcgag atccgctcct
     2821 tcatcgcccg gcacgccctc tttccggcac accgccgcca gggtgccgcc gagcagcttc
     2881 gcggcacgcc acgccga
//
LOCUS       DASTMK010000320         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_496351, whole genome shotgun sequence.
ACCESSION   DASTMK010000320 DASTMK010000000
VERSION     DASTMK010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496351"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFQ79_13885"
     CDS             complement(<1..430)
                     /locus_tag="VFQ79_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0140807.1"
                     /translation="MRQNTTSSGRYKWYVVAMLWCISFFNYADRQAIFSVFPLLEKEM
                     GLTPVELGLLGSSFAWVYGLSAPFAGLVVDRIRRKTAILLGLHIWSIICMATALSKNF
                     KHLFFFRAAEGLGETVYYPASMSMVSDYHGKETRSRAMGLH"
     gene            complement(461..1702)
                     /locus_tag="VFQ79_13890"
     CDS             complement(461..1702)
                     /locus_tag="VFQ79_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140808.1"
                     /translation="MKSVRYTRYTGDDFGIGAEDLMKALSDFFLSSGFEDQYMRFSEW
                     NEHTLEELKEALQKALESGQLFDQQRAEEIAEQLQSMSPEQLDQLLDRLVQKLIDEGY
                     LSMDQQPQAEPQGAPGQIGGAENKVKFEITSKSVDFLGFKTLKDLLASLGKSSFGAHD
                     TRDLATGIEASGASRRYEFGDTMNLDVSETLFSAIKREGVKVPLELDYNDLHVHQAEY
                     QSSCATVLMLDCSHSMILYGEDRFTPAKKVALALAHLIRTQYPGDSLKCVLFHDSAEE
                     LRLSDLARVQVGPYYTNTRDGLILAQRLLSQEKKDMKQIIMITDGKPSCLTLEDGRIY
                     RNAFGLDPLVISRTLEEVNRCKRQGILINTFMLASDYGLVNFVQRVTELCRGKAYFTT
                     PYTLGQYLLMDYMTRKTRTVH"
     gene            complement(1817..3211)
                     /locus_tag="VFQ79_13895"
     CDS             complement(1817..3211)
                     /locus_tag="VFQ79_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEU0140809.1"
                     /translation="MRSNLICLLQRKEPLFSGIVGYDDTVVPQVINAILSRHNFILLG
                     LRGQAKTRLIRMLATLLDEAVPYVAGCEIHDNPYQPICRNCRNLIEEMGEETPIAFLS
                     PEDRYVEKLATPDVTIADMIGDIDPIKAARRGHNISDELTVHYGLLPRANRGIFAINE
                     LPDLAGKIQVGLFNIMQEGDVQIKGYPVRLPLDLMLVFTANPEDYTARGKIITPLKDR
                     IGSEIRTHYPVTLEQGMEITRHESWTDRDAPVTLLIPDFIRQVVEELAFLAREDKKVD
                     KRSGVSQRLPISVLENVASNAEQRALRMGEDTAVPRVMDLYAALPSITGKLELEYEGE
                     LKGGDAVARELIRTAVGKVYNHYMEGVNVSHIVQWFEIGGSLKLDELSGSADLLSRLS
                     GIQGLLEKTGAFGLGSSEPDALRISAAEFILEGLYAHRRLSRSEERGFTAEERKRETP
                     APEEGRRTRRQHFN"
     gene            3441..>3807
                     /locus_tag="VFQ79_13900"
     CDS             3441..>3807
                     /locus_tag="VFQ79_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0140810.1"
                     /translation="MNPDLPDMDFQRFWRKLQQIGLNAYEARSYLVLVGHPRFKALEL
                     AARAHVPRQKIYEVLDSLMEKGFAQVVQEKTKLFSAVEPGLAIPGYLARRAEMLEHEL
                     TEQARMAGDLVDDLMSAYST"
BASE COUNT          777 a         1107 c         1088 g          835 t
ORIGIN      
        1 gatgcagccc catcgcccgc gagcgggtct ccttgccgtg gtaatcgctg accatcgaca
       61 tggaggcggg ataataaact gtctcgccaa gcccttcggc cgcgcggaag aagaacaggt
      121 gcttgaaatt cttcgataac gccgtggcca tacaaatgat gctccaaata tgcagcccca
      181 gaaggatggc cgtcttgcgg cgtatgcgat ctacgacgag tcctgcaaac ggagcactga
      241 gaccatagac ccacgcgaaa gaggagccga ggagacccag ttccacgggc gtcagcccca
      301 tttctttttc aagcaggggg aacacggaga aaatggcctg ccggtccgcg taattgaaaa
      361 aggagatgca ccacagcatc gccaccacgt accacttgta gcggccggac gacgtcgtat
      421 tctgtcgcat tctttgttcg tggggacggt atcgtgccgc ttaatggacc gttcgcgtct
      481 tgcgggtcat gtagtccatc agcagatact gtcccaacgt ataaggcgtg gtgaaatagg
      541 ccttaccgcg gcacagctcc gtcactctct gaacaaagtt taccagccca tagtcgctgg
      601 cgagcatgaa ggtgttaatc agaataccct gccgcttgca gcggttcact tcttccagcg
      661 ttctgctgat aaccagcggg tccagaccga aggcattgcg ataaatgcgc ccgtcttcaa
      721 gcgtcaggca ggaaggcttc ccatccgtga tcatgatgat ctgcttcatg tccttctttt
      781 cctgactgag cagcctctgc gccagaataa ggccatcgcg cgtattcgtg tagtaaggcc
      841 ctacctgcac acgggccaga tcggagagac gaagctcctc ggcggaatcg tggaacagga
      901 cacatttcag gctgtcgccc ggatactgtg tacggatgag gtgcgccagc gccagagcga
      961 ccttcttggc gggcgtaaag cggtcttcgc catacagaat catgctgtgg ctgcaatcga
     1021 gcatcagcac ggtggcgcac gagctctggt attccgcctg gtgcacgtgc aggtcgttat
     1081 agtcgagctc cagcggcacc tttacacctt cccgcttgat ggcggagaaa agcgtttcac
     1141 tgacatccag gttcattgtg tcgccgaatt catagcgccg cgaggcgccg ctggcctcga
     1201 tgccggtggc caggtcgcga gtgtcgtgag cgccgaagct ggacttgccg agcgaagcca
     1261 ggaggtcttt cagggtcttg aatccgagga agtccacgga cttgctggtg atttcgaact
     1321 tcaccttgtt ttcggcgccg ccgatctggc cgggcgcgcc ctgcggctcg gcttgcggct
     1381 gctggtccat ggacagatac ccttcgtcga tcaacttctg aaccagccgg tccaggagtt
     1441 gatccaactg ttccggcgac atgctctgga gttgctcggc aatctcttcc gctcgctgct
     1501 ggtcgaacaa ctgcccggat tccagcgcct tttgcagcgc ctccttcaac tcttccagcg
     1561 tgtgctcgtt ccactccgag aagcgcatgt actggtcctc gaagccgctc gagaggaaaa
     1621 aatccgagag cgctttcatc agatcttcgg cgccgatgcc gaagtcatct ccggtgtagc
     1681 gggtatatcg aacagatttc acagacccct ccccaggcga aaccgcttgc tgacgcgcgc
     1741 ggctccgttt ccgagccgtg accgcgaggg agcggccccg ttggaaatac gtaaacgaac
     1801 ttctgaaacc atgtgctcag ttgaaatgct gccggcgagt gcggcggcct tcttccggcg
     1861 ccggtgtttc gcgctttcgc tcttccgcgg taaagccgcg ttcttcactg cggctcagcc
     1921 ggcggtgtgc gtagagacct tcgaggatga actccgctgc tgagatgcgt agcgcatccg
     1981 gctcactgga gccgagcccg aacgcaccgg ttttttcgag cagtccctga atgccggaaa
     2041 ggcgtgaaag cagatccgct gaaccggaaa gctcgtccag cttgagcgat ccgccgatct
     2101 caaaccattg cacaatgtgc gatacgttca cgccttccat gtaatggttg taaactttcc
     2161 ccaccgcggt gcgaatcagc tcccttgcaa cggcgtctcc gcctttcaat tcaccttcat
     2221 actccagttc aagcttgccg gttatggaag gcagcgccgc gtaaagatcc atcacccgcg
     2281 gaacggcggt gtcttctccc atccgcaagg cgcgctgttc ggcgttggaa gcgacgtttt
     2341 ccagaaccga aatcggcaat cgttgcgaga cgccggagcg cttgtccacc ttcttgtcct
     2401 cgcgcgccaa aaacgccagc tcttcgacca cctgccggat gaagtccgga atcagaagcg
     2461 tcacaggagc gtcacggtcc gtccacgatt cgtgccgggt aatctccatc ccctgctcca
     2521 gcgtcacggg gtagtgtgtg cggatctcgc ttccgatgcg gtctttgagc ggcgtgatga
     2581 tctttccgcg cgccgtataa tcttccggat tcgcggtgaa caccaacatc aagtcgagcg
     2641 gtagccggac agggtaacct ttgatctgga cgtcgccctc ctgcatgatg ttgaacaggc
     2701 caacctgaat ctttccggcc aggtcgggga gttcgttgat cgcgaaaatg ccccggttgg
     2761 cgcgaggcag aagcccgtag tgcacggtca gctcgtcgga gatgttgtgt ccgcggcggg
     2821 ccgccttgat cgggtcgata tcgccgatca tgtcggcaat cgtcacatcc ggcgtggcga
     2881 gtttttcaac ataacggtct tccggcgaca gaaacgcgat cggagtttcc tcccccatct
     2941 cttcgatgag gttccggcag ttgcggcata taggctgata cgggttgtcg tggatctcac
     3001 atccggccac gtacggtaca gcttcatcca gaagcgtagc cagcatgcgg atgagccgcg
     3061 tctttgcctg gccgcgcagg ccgagcagga taaaattgtg acgcgagagt atcgcgttga
     3121 tgacctgagg tacaacggta tcgtcgtagc ctacgatacc ggagaagagc ggttctttgc
     3181 gttgcagcaa gcagatgaga ttgctgcgca tctcatcctt gaccgttcgt gccgcaactc
     3241 tctcctcgga aaatttgctt cgtctaagtg ctccaagagt gtgaggtaat tcccagtggg
     3301 tggttttcat cactgcccat cccgatctac ggcgattgta gcaccgcaat cgatgagatt
     3361 tgatttcctg ttctgtcaga cgcacgaaaa gggaatccgg aaaccggaat cgaaacaaat
     3421 ctgatatctt tgggactcaa atgaaccccg atttgcccga catggatttt cagcgcttct
     3481 ggcggaagtt gcagcagata ggccttaacg cttatgaagc gcgctcgtac cttgtcctcg
     3541 tcggccaccc gagatttaaa gcgctcgaac tggctgcgcg cgctcatgtg ccccggcaga
     3601 agatttacga ggtgctcgac agtctgatgg aaaagggctt cgcgcaggtg gttcaggaaa
     3661 agacgaagct cttttcagcg gtagagcccg gattggcgat acccggctac ctggcccggc
     3721 gtgccgaaat gctggaacat gagttgaccg aacaggcgcg catggccggc gatctggtgg
     3781 acgatctcat gtccgcgtac tcgaccg
//
LOCUS       DASTMK010000321         4628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_558372, whole genome shotgun sequence.
ACCESSION   DASTMK010000321 DASTMK010000000
VERSION     DASTMK010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558372"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VFQ79_13905"
     CDS             complement(<1..1004)
                     /locus_tag="VFQ79_13905"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEU0140811.1"
                     /translation="MPASANALHRALNFVEYAGNKLPHPASLFAIFALLVIALSWLLV
                     QFGLTAVHPGTGKEINAVNLLSDGGLRRMLTSLVTNFTGFVPLGTVLVAMLGVGVAEG
                     SGLLGAALKGMVLAAPKRLITVVVVFTGVMSNAASEAGYVILIPLAAVIFLAVGRHPL
                     AGLAAAFAGVSGGYSANLVLGTVDPLLSGLTTEGARIIDPAYYVNPAANYYFMAISTF
                     LITMAGTIVTHRLVEPRLGKFEGDAGAKIEKLGPGEKRGLKAAGFALLVTNVLLVLSV
                     VPESGILRNPETGSILDSPFLSGIVAIIFLMFLIPGLAYGIAAGTIRNDTDVVNFMGK
                     T"
     gene            1092..2504
                     /locus_tag="VFQ79_13910"
     CDS             1092..2504
                     /locus_tag="VFQ79_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140812.1"
                     /translation="MTDKRGPSRREFLGKQASLAASLSLASGAYAKAPKFTPKANTRV
                     LGANDRINLGFIGNGMQFHALVEGAFRARKQQKNDFEFAAVCDVWEPRLKYAQEQTKA
                     EKTYRDYRELLSNPDIDGVVIAVPDQWHFLMASQACQCGKDVYLEKPMTYTVAEAAKL
                     NEIVGDTKRVLQVGGTGPSTRLYWKVNEYIRSGKMGKVVWGLISYNRNTREGMWDYPI
                     PGVGSDHWPDAEVSPKNLDWEMWLGPAPHRKFSKERYFRWRKYWDYSGGNATDLLYHR
                     LGMMSTMIGFDFPTRVVGAGGIYVQKNREVPDTYMTLIEYPGEYAVNMVSCMANSRSV
                     PMTVYSNWATLEITEPQMAANAMGDQRRGTAAPRRAQVGLVRPERTFLKEFQEANDGK
                     TEVVIESEPSASLADDWLDCMRSRGTPVYNVLRGYQVMTAIALGVESYRSGKVMAFDP
                     ATQRVLSSPPSHKEYLPADA"
     gene            complement(2518..3264)
                     /locus_tag="VFQ79_13915"
     CDS             complement(2518..3264)
                     /locus_tag="VFQ79_13915"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0140813.1"
                     /translation="MRLFAAGIMTVGLALTTGLSPAGAQTAPPGFTSLFNGKDLTGWK
                     IPEGDNGHWKVVNGVIDYDARSEAPKDKNLWSEKSYKDFVLRVDWRITATPFRNKDVR
                     IVMPDGSEKLDENGQVIKIEVPDSDSGIFIRGSGKAQVNIWNWPVGSGEFYGYRTDRN
                     MPQAIKAAVTPKYNADKNIGEWNTFEITMKGDRVTVVLNGKTVVENAQLPGIPAEGPL
                     ALQHHGTFRDGRFLGPPSLVQFRNIFIKEL"
     gene            3353..3748
                     /locus_tag="VFQ79_13920"
     CDS             3353..3748
                     /locus_tag="VFQ79_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0140814.1"
                     /translation="MPKITFLPQNVTVEFDPETMPYADHGKPLSILDIALNNGVQLEH
                     ACGGFCACTTCHVVVKEGREHLSEMDDEEADRLDMAADLQLHSRLGCQAVYEGNGDIV
                     VEIPAWNRNYVSEGGGSILGEAIPAGKRD"
     gene            complement(3762..>4628)
                     /locus_tag="VFQ79_13925"
     CDS             complement(3762..>4628)
                     /locus_tag="VFQ79_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140815.1"
                     /translation="CGSRDTQSVRIDPALLTLVPPDTIAMGGARIDQIKKTTVYSKYI
                     AQRPFPAFDALRERLGIDAREDLWEVLAASDGKDTVLFARGRFSPQGIEPKLEKEGGR
                     RTQYKGYTLVGSDEAAITFMNTSTAVAGPVRVVQSVIDQRDSGAKPPAALLDRTKQIP
                     AGAQLWAVSSGAVSRLPPGVVGEGNLANLGKVFRSLDGFSGWADLSSGFHLNASGTSA
                     TEQDAKKLHDAIRGLLGLARLNTPDGRTDLLRVYDQIDVSMEERTVKIEADLPMELVE
                     KLAATAEEFRRR"
BASE COUNT          979 a         1392 c         1346 g          911 t
ORIGIN      
        1 gtcttgccca tgaaattaac tacgtccgtg tcgttgcgaa tagtgccggc ggctataccg
       61 taggccagtc cgggaataag gaacatcagg aagataatgg ccacgatgcc cgagagaaaa
      121 ggggaatcga gaatgctacc cgtctcggga ttgcgaagaa taccgctttc gggaacgacc
      181 gacagcacca ggagtacatt cgttacgagg agtgcaaagc ccgccgcctt cagccctcgc
      241 ttttcgcccg gccccagctt ttcgatcttc gcgccggcgt cgccttcgaa cttgcccagc
      301 ctgggctcca ccagcctgtg cgtcacaatg gtcccggcca tggtgatgag gaatgtcgaa
      361 atcgccatga agtagtagtt cgccgcggga ttgacgtagt acgcaggatc gatgatccgc
      421 gccccttcag tcgttagccc gctcaacaga gggtccacag ttcccagcac gaggtttgcg
      481 ctgtatcctc cggagacacc ggcgaatgca gcggcaagtc cagcgagcgg atgccgcccc
      541 acggccagga agatcaccgc cgccagcgga atcaggatga cgtaacccgc ctccgatgcc
      601 gcgttcgaca tcacaccggt gaacaccacc acgacggtga tcaggcgctt cggcgcggcg
      661 agcaccattc ccttaagagc ggcgccgagg agtcccgagc cttccgccac cccgacccca
      721 agcatcgcga ccagcactgt ccccaatggt acgaaccccg tgaagttggt caccaggctg
      781 gtcagcatcc tcctgagacc gccgtcggac agcaggttga cggcgttgat ctctttgccc
      841 gtgccggggt ggacagccgt caggccgaac tgcaccagca gccacgagag ggcgattacc
      901 agaagcgcga agatcgcgaa gagagaagct gggtgaggca gtttgttacc ggcatactcg
      961 acaaagttga gcgcgcggtg aagtgcgttg gcggacgcag gcataaacgc atgatcgcac
     1021 gcacgccgct ccggaacgcc cgcggcactg ggatatgatt ggggtaacga aattgatatt
     1081 caggagcaat catgaccgat aaacgtggac cgagccgtcg cgaatttctc ggcaaacagg
     1141 cgtcgctggc cgcatcgctt tcgttagcct ccggagcgta cgccaaagcg ccgaagttca
     1201 cacccaaagc caatactcgc gttctgggtg cgaacgatcg catcaacctg ggttttatcg
     1261 gaaacggaat gcagttccac gccctggtgg aaggcgcgtt ccgtgcccgc aaacagcaga
     1321 agaacgattt cgagttcgcc gccgtatgcg atgtgtggga gccccggctg aaatatgcgc
     1381 aggaacagac gaaagccgaa aagacatacc gcgactaccg cgaactgtta tcgaatccgg
     1441 acatagacgg tgtggtgatt gccgttccgg accaatggca ttttctgatg gccagccagg
     1501 cctgccagtg cggcaaagac gtgtatcttg aaaagccgat gacgtacacg gtagccgaag
     1561 cggccaagct gaacgagatc gtcggcgata caaaacgcgt cctgcaggta ggcggaaccg
     1621 gtcccagcac ccgcctttat tggaaggtga acgagtacat ccgttccggc aagatgggca
     1681 aggttgtctg gggtctcatc agctataacc gcaacacgcg cgaaggcatg tgggattatc
     1741 cgatccccgg cgtcggcagc gatcactggc cggatgccga ggtcagcccg aagaacctgg
     1801 attgggaaat gtggctgggt cccgcgccgc accgcaaatt cagcaaggag cgatacttcc
     1861 gctggagaaa gtattgggac tattcgggcg gtaatgcgac cgatcttctc taccatcgtc
     1921 tgggaatgat gagcacgatg atcggcttcg atttcccgac gcgcgtcgtg ggcgccggcg
     1981 gcatctatgt gcagaagaac cgcgaggttc cggataccta catgacgctc atcgagtatc
     2041 cgggcgagta cgccgtcaac atggtgtcgt gcatggccaa ctcccgatcc gtgcccatga
     2101 cggtctacag caattgggcc acgctcgaaa ttacagaacc gcaaatggca gcgaacgcca
     2161 tgggcgacca gcgccgcgga acggccgcgc cgcgacgtgc acaagtgggg ctggtccggc
     2221 cggagcgaac gttcctgaag gaattccagg aagcaaatga cgggaaaacg gaggttgtca
     2281 ttgagtccga gcccagtgcc agcctcgccg acgactggct cgactgcatg cgcagccgcg
     2341 gcacaccggt ctataacgta ttgcgcgggt accaggtgat gacagcgatc gcgctcggtg
     2401 tggaatcgta ccgcagcggc aaggtgatgg ctttcgatcc cgccacccag cgcgtgctat
     2461 cgtcaccgcc gtcacacaaa gagtacctgc ccgcggacgc ttgacgcgta actcgtacta
     2521 cagttccttg atgaagatat ttcggaactg taccaggctc ggcggaccta gaaaccgccc
     2581 atcccggaaa gtgccgtgat gctgcaaggc aagcggccct tccgcgggta tgcccggtaa
     2641 ctgtgcattc tcgacaacgg tcttgccgtt cagtaccacg gtgacgcgat cgcctttcat
     2701 cgtgatctcg aacgtgttcc actcgccgat gtttttgtcg gcgttgtact tcggtgtaac
     2761 cgcggctttg atggcctgcg gcatgttgcg atcggtgcgg tatccgtaga attcgccgga
     2821 acccaccggc cagttccaga tgttgacctg cgccttgccg cttccgcgta taaaaatacc
     2881 cgaatccgaa tcgggaactt cgatcttgat cacctggccg ttctcgtcca acttttcgga
     2941 gccgtccggc atcacaatgc gaacgtcctt gttgcgaaaa ggagtggctg tgatgcgcca
     3001 gtctacacgg agcacaaaat ccttgtacga cttctcactc cagagattct tgtctttggg
     3061 cgcttcgctc cgcgcgtcgt aatcgatgac gccattcacc actttccagt ggccgttatc
     3121 cccctcggga atcttccaac ccgtcagatc ctttccgttg aacagggacg tgaaacccgg
     3181 cggcgctgtt tgggcgccag cgggagacag ccccgtcgta agagccaaac ccactgtcat
     3241 gatgcctgct gcgaaaagtc gcatttatcc tcccgtacaa agatatgaaa tcattcttta
     3301 cgtgctggaa caaatccgac tgattaggtc gcatatctgc tatactggtg ccgtgcccaa
     3361 gatcaccttt ctgcctcaga atgtcacggt ggagttcgac cccgagacca tgccctacgc
     3421 cgatcacggg aaaccgctct cgattctgga cattgcgttg aacaacgggg tgcagctcga
     3481 acacgcttgc ggaggattct gcgcctgcac gacgtgccac gttgtagtga aagagggcag
     3541 ggaacatctt tccgagatgg atgacgagga agccgaccgc ctggatatgg ccgcggatct
     3601 gcaacttcat tcgcggttgg gctgccaggc ggtttatgaa ggcaacggcg atatcgtggt
     3661 cgagattccc gcctggaatc gcaactacgt gagcgaaggg ggcggctcga tcctcgggga
     3721 agcgattccc gccggcaagc gggactagct acaatccgtc ttcaccggcg gcggaactcc
     3781 tcagccgtcg ccgccagttt ctccacgagt tccatgggaa gatccgcctc gatcttcacc
     3841 gtgcgttcct ccatgctgac gtcgatctgg tcgtataccc gcagtaagtc cgttcgccca
     3901 tccggagtgt tcaaccgcgc caatccgagc aatccgcgaa tggcatcatg cagctttttg
     3961 gcatcctgtt ccgtcgcgct ggtgcccgat gcgttgaggt gaaacccgga gctcagatcg
     4021 gcccatccgc tgaacccgtc gagagagcgg aatacttttc caagattcgc caggttaccc
     4081 tcgccgacga ctccgggcgg caaccggctg actgcgccgg aagagaccgc ccacaactgt
     4141 gcgccagcgg ggatctgctt cgtgcgatcc agcaaagccg cgggcggctt tgcacccgaa
     4201 tccctctgat cgataacgga ctgcacgacg cgcaccggac ccgcaaccgc cgtcgatgta
     4261 ttcatgaacg tgatagcggc ttcgtcgctt cccacgaggg tatagccttt gtattgggtc
     4321 cgccggcctc cttctttttc aagtttcggc tcgatacctt gcggagagaa gcgtccgcgc
     4381 gcgaatagca ctgtgtcttt cccgtccgac gccgcgagca cctcccaaag atcttcccgc
     4441 gcatcgatgc caagccgctc acgcagtgcg tcaaaagccg ggaatggccg ctgtgcgatg
     4501 tacttgctgt agactgtggt cttcttgatc tggtcgattc ttgcgccgcc catcgcgatg
     4561 gtgtccggcg gcaccagtgt gagcagcgct gggtcgattc tcacggactg tgtatccctg
     4621 gagccgca
//
LOCUS       DASTMK010000322         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_591826, whole genome shotgun sequence.
ACCESSION   DASTMK010000322 DASTMK010000000
VERSION     DASTMK010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591826"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1028)
                     /locus_tag="VFQ79_13930"
     CDS             complement(87..1028)
                     /locus_tag="VFQ79_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEU0140816.1"
                     /translation="MQSELMFGTQQEAWPALPFQAWRDTCTTLQMWTQVIGKVRMALS
                     APVNHWWHIPLYVSVRGLRTSSIPYRTGAFEIEFDFVSHDLRFSTSWGLARSIPLRSM
                     TVADFYRASMDTLQELGVAVSVWPVAVEMEHPVRLDRDSDHAAYDAEYANRFWRVLLQ
                     SDRVFKQFRSDFLGKVSPVHFFWGAFDLAVTRFSGRAAPEHPGGIPNVGDFVMREAYS
                     HEVSSAGFWPGGGALDEPAFYSYAYPEPPGFKDQPVRPKEAHYHEQMREFLLPYEAVR
                     TAADPDTALLEFLQSSYEAAADLAKWDRAAVERSPVE"
     gene            complement(1058..1840)
                     /locus_tag="VFQ79_13935"
     CDS             complement(1058..1840)
                     /locus_tag="VFQ79_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEU0140817.1"
                     /translation="MSKLLEGKTALVLGVANRWSLAYAIAQAFSREGAKVLLTYQSER
                     QRLTVEELANELSAASVFPCDVTKDEEIAALTESLRKNGHVAIDTVVHSIAFANRDDL
                     TRPFVETSRSGFLLAQEVSAYSLVAVAKATAPLMAAGGSIMTLTYLGATRVVQNYNVM
                     GVAKAALEASVRYLANDLGPGNIRVNAISAGPVKTASARGIKDFSKVLDSVERVAPLR
                     RNTDPGEVADTAVFLASDLGRGVTGNILFVDAGFQVIGLTAG"
     gene            complement(1888..2823)
                     /locus_tag="VFQ79_13940"
     CDS             complement(1888..2823)
                     /locus_tag="VFQ79_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140818.1"
                     /translation="MKYLLALALISVPSFAADLRLGIIGTDTSHVIAFSSVLNNPSHP
                     EHIPGARIVAAYKGGSSDVESSYTRVDKYAEELKAKWNIEIVPDIPTLCSKVDAILLE
                     SVDGRKHLEQAKQVIAAKKPMFIDKPLASTLEDAREIARLAKAAGVPWFSTSSLRWSE
                     IVETMKTSDAKAVVTWGPGPTEAHHYLDLSWYAIHPVEMLFAMMGPGCQEVTRVSGEG
                     IDSVTCRWKDGRIGTVNALRPYGGYGAVVYREKDVVTSPPKAKSSYTPMLKHIVEFFQ
                     TGKPPVPNEETLEIFAFMDAAQRSKEAGGRPMKLR"
     gene            2850..>4071
                     /locus_tag="VFQ79_13945"
     CDS             2850..>4071
                     /locus_tag="VFQ79_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0140819.1"
                     /translation="MDLATPLMYVKGVGPARAAMLEAKGLLTVEDLLYYPPFRYEDRR
                     NVKPVSALAPGEMATVIAEVRSARLAGFQRRNLGMFQATFADGSGARLIGKWFHGGYL
                     QSVITPGLKIALYGKIEIDSYTGDLVTLHPEFEILSEEDEDAEAALHLGRIVPIYEAP
                     GKLTTRLMRTIVYRALERVSPVEDHLPAGIRQRLKLRDFWPAILEMHFPAADADVRLL
                     NAFRSPAQFRLIFEEFFWLECGLALKRSRARLEQGISFDLNDRVREKIRAMLPFKPTG
                     AQKRVLKEIAEDMAAPRPMNRLLQGDVGSGKTLVAAEAAIIAIENGYQVAVLAPTEIL
                     AGQHHFYFKNLFTKLGYVVALLTGSSTQREKTQIKKLVAAGMVHILIGTHAVIEADVE
                     FHKLGLAVIDEQHRF"
BASE COUNT          823 a         1238 c         1194 g          816 t
ORIGIN      
        1 ttctcgttgg gtcacgcttg acccccgcta taaggatatc gcagctcggt tcctacttgg
       61 tcggctatcg cgacggcggt tctgttctat tcgacaggcg aacgttccac cgccgcgcga
      121 tcccactttg caaggtccgc cgcggcttcg taagagcttt ggaggaattc gagcaaagcg
      181 gtatcgggat cggccgcggt tctcacagct tcgtagggca ggagaaactc gcgcatctgc
      241 tcgtgatagt gagcctcttt gggtcttacc ggctggtctt tgaagccggg cggctccggg
      301 taggcgtagg aatagaaagc aggttcatcg agggcgcccc ctccaggcca aaagccggca
      361 ctgctgacct catgcgaata cgcctcacgc atcacgaagt cgccgacgtt tggaatgcca
      421 ccggggtgtt cgggagcggc gcggccggaa aagcgtgtga ccgccaggtc gaacgcgccc
      481 cagaaaaaat gaaccgggct gactttgccg aggaaatccg accggaactg tttgaatacg
      541 cggtccgact gcagaagaac acgccagaac cggtttgcgt attcggcatc gtaggcggcg
      601 tgatcggaat cgcggtccag ccggaccggg tgctccattt ccacggctac gggccaaacg
      661 gaaacggcaa cgccgagctc ctgcaaggtg tccatagatg cacggtagaa gtcggccacg
      721 gtcatggacc gcaaaggaat ggagcgggca agcccccaac tggtggaaaa acgtaagtcg
      781 tggcttacaa aatcgaactc gatctcaaaa gcgccggtgc ggtaagggat ggaggatgtc
      841 ctcaagccgc ggacgctgac ataaagcgga atatgccacc aatgattgac cggcgcactg
      901 agcgccattc ggaccttgcc gatcacctga gtccacattt gaagtgtggt gcaagtgtcc
      961 cgccaagcct ggaacggcaa tgccggccaa gcctcctgtt gcgttccgaa catgagctcc
     1021 gattgcatga ttcccctccc gaagcgggcg gccggcttca gcccgcggtc agaccgatca
     1081 cctggaaccc ggcatccacg aacaggatat tgcctgtaac gccgcggcca agatcgctcg
     1141 ccagaaacac ggccgtatcg gccacttctc caggatcggt gttccggcgt aacggcgcca
     1201 cccgctccac ggaatccaac accttcgaaa aatccttgat gccgcgcgcg gaagcggtct
     1261 tcaccggtcc ggccgaaatc gcattcacgc ggatgttgcc ggggcccaga tcatttgcca
     1321 gataccggac cgacgcctcc agcgccgcct tggcaacacc catcacattg taattctgca
     1381 cgacccttgt tgcgcccaga taggtcaggg tcatgatgga accgcccgcc gccatcagcg
     1441 gagccgtggc tttggccacc gccaccagcg aataggcact gacctcctgc gccagcagaa
     1501 agccggagcg ggacgtttcc acaaacggac gcgtcaaatc gtcgcgattg gcgaacgcga
     1561 tactgtgcac tacagtgtcg atggcgacat gcccgttctt ccggagggat tccgtcaggg
     1621 cggcaatctc ttcgtccttg gtcacgtcgc agggaaatac gctcgcggcc gacaactcgt
     1681 ttgcgagctc ttctactgtg agccgctgcc gctcgctctg ataggtgagg aggaccttgg
     1741 cgccctcgcg tgagaatgcc tgcgcgatgg cgtaagccag actccacctg ttggcgacac
     1801 cgagaacgag cgcggttttc ccttccagca attttgacat gaactcgcta ttctaccgcg
     1861 agcgttggtg cgaactctcc gcagccctta tcgcagcttc atcgggcggc cgcccgcttc
     1921 cttactgcgc tgtgccgcgt ccatgaacgc aaagatttcc agcgtctcct cgttcggtac
     1981 gggcggctta ccggtctgga aaaactcgac gatgtgtttc agcatcggcg tatagctcga
     2041 cttcgccttc ggagggctcg ttacgacatc cttttcgcgg tacaccaccg cgccatagcc
     2101 gccatacggc cgcaaggcgt tcacagtgcc aatgcgtccg tccttccagc ggcacgttac
     2161 ggagtcgata ccctccccgg aaaccctcgt aacttcctgg cagcccggac ccatcatcgc
     2221 gaacagcatc tcgaccgggt gaatagcgta ccacgagagg tcgagataat ggtgcgcttc
     2281 ggtgggtcct ggaccccatg taaccaccgc cttcgcgtca gaggtcttca tcgtctctac
     2341 gatctcgctc catcgcaggc tggaggtgct gaaccagggg actccggccg ccttcgcaag
     2401 ccgcgcgatc tcgcgggcat cctcgagtgt ggaagccagc ggcttgtcga tgaacatagg
     2461 tttcttcgcc gcaatcacct gcttggcctg ctccaggtgc tttctcccat ccacgctctc
     2521 aagcaggatt gcatcaacct tgctgcacag ggtgggaatg tcgggaacaa tttcgatatt
     2581 ccacttcgct ttcagttctt ctgcgtactt atccactctc gtatagctgg actccacatc
     2641 cgagctgccg cctttgtatg ccgccacgat gcgggcaccc ggaatatgtt ccggatgcga
     2701 cgggttgttc agcacgctgg aaaaggcgat gacgtgggac gtgtcggttc cgatgatgcc
     2761 gagtctcagg tcggccgcaa aggatggcac ggagatcagt gcgagcgcta gcaggtattt
     2821 catcaaccgc attgtacact agtgtgttct tggatctggc cactccattg atgtatgtga
     2881 agggcgtggg ccccgcgcgg gcagcgatgc tcgaagccaa ggggctgttg acggtagaag
     2941 atcttctgta ttaccccccg ttccggtatg aagaccggcg caatgtgaag ccggtgtccg
     3001 ccctcgctcc cggcgagatg gcgaccgtaa tcgccgaggt ccgctctgcc cggctcgccg
     3061 ggtttcaacg ccgcaacctg ggaatgtttc aggccacttt cgctgatggc tccggcgcac
     3121 gcctcattgg gaaatggttt catggcggct acctgcaatc ggtgatcacg ccggggctca
     3181 aaatcgctct ctacgggaag atcgaaatcg acagctatac gggcgacctc gtaacgctgc
     3241 atcccgagtt cgaaattctt tccgaagaag acgaggacgc cgaagccgcg ctgcacctcg
     3301 gccgcattgt tcccatctac gaggcgccgg ggaagctcac aacccggttg atgcgcacga
     3361 ttgtgtaccg ggcgctggag cgggtttcgc cagtggagga ccaccttccg gccggtatcc
     3421 gccagcgtct gaaactgcgg gatttttggc cggccatcct ggagatgcac tttcccgccg
     3481 cggatgccga cgtccggctg ttgaacgcct tccggtctcc agcacagttc cggttgattt
     3541 tcgaagagtt cttctggctg gagtgcggct tggcgctgaa gcgctcccgg gcgcggctgg
     3601 agcagggcat cagcttcgat ctgaacgacc gcgtacgcga aaagatcaga gcaatgttgc
     3661 cgttcaagcc cacgggagcg cagaaacgcg tgctaaagga aatcgcggaa gacatggcgg
     3721 cgccgcggcc catgaaccgt ttgctgcagg gcgatgtcgg cagcggcaaa acactcgtcg
     3781 cggccgaggc ggccattatc gcgatcgaga atggttacca ggtggcggtg ctcgctccaa
     3841 ccgaaattct ggccggacag catcacttct atttcaagaa cctgttcaca aaactgggct
     3901 atgtcgttgc gctgttgaca ggctcgtcca cccaacgcga gaaaacacaa atcaaaaaac
     3961 tggtggctgc cggaatggtg catattctca tcggtaccca cgcagtgata gaggccgatg
     4021 tcgaattcca caagttgggc ttggcggtca tcgacgaaca gcaccggttc g
//
LOCUS       DASTMK010000323         7743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_17344, whole genome shotgun sequence.
ACCESSION   DASTMK010000323 DASTMK010000000
VERSION     DASTMK010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17344"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4006)
                     /locus_tag="VFQ79_13950"
     CDS             complement(<1..4006)
                     /locus_tag="VFQ79_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU0140820.1"
                     /translation="MTASELLSYLRSLDVKLWLEGDRLRYKAAKGVLTPTLLADMAER
                     KAELIAHLREALSGQPSLAPAIVPVPRTPSGLPVSFSQQRIWFVDQLAPGNSFFNIDS
                     AIRLKTPIDPGILERSLNEIVRRHESLRTTFQLINDEPAQVIAPSLELPLPVIDLRHL
                     APAEREAEALRIATEESRKPFDLSCGPLLRTTLVWLGYSDYVFLLTMHHIVSDGWSMG
                     VFFQELNTLYGAFLLGRPSPLPPLEIQYGDFAAWQRQWLRGAVLERQLNYWKQQLQDI
                     PTLQLPTDRPRPAMQTFRGAYHSVTLSSALTAPLKALCHKEGATLFMVLLAAFQALLY
                     RYTGQDDIVVGSYIANRNRGEIEKLIGFFVNTLVFRTDCGGDPEFRELLRRVREAALG
                     AYAHQDVPFTKLVEELAPDRDLSRNPLFQVVFQLFNAPTVSLQDSDSGSPNLAVERGT
                     AIFDLALHTFETPSGIHVQFEYNTDLFDAGTIERMAGHYETLLEGIAANPDQRLAALP
                     LLTQAERRQLLIDWNQTDRAYPCDRSLVEQFETQVAAAPTASAFLCGDQRLTYEDLNR
                     RANQTAHYLKSMGVEAGSTVGICLERSLDFVVGLLAIFKAGCVYLPLDPTYPRERLAF
                     MLKDSRAGALITKRALLPALAEVPNRTICLDDDARAIAGHSGDNPACTVGPDDMAYLI
                     YTSGSTGRPKGVAVEHRQILNRLDWMWRDYPFTREEVLSQKTAISFVDSIWEFFGGLL
                     QGVPAAIIPDAVLKDPFALVEELAGRNVTRIWVVPSLLRAMLDTVPDLQHRLPALRFW
                     VSSGEALPADLFELFRRQMPHATLYNLYGTSEFWDATWYIPAEQNGNVRQVPIGRPIQ
                     NMQIYVLDSNGQPVPISVPGELHVGGVGLARGYLNRPDLTGAKFVPNPFEGAAQRLYK
                     TGDLVRYLPDGNLEYLARMDQQIKLRGFRIELGEIEAALLQHPGVQSAVVDARADATG
                     EQRLVAYIVQDPRYTGAGLQQDNESWSGDRVPEWQAVWDETYIQAGGEQDPTFNISGF
                     NSSYTGRQIPAGEVREWVGQAVDHVLSLKPRRVLEVGCGAGLLLFRLAPHCDEYFGTD
                     FSAAALNYIERHAAAMKNIRLFQSNADDWSFLKEESFDLVLLHSVIQYFPSMDYLLRV
                     LEGAVAAANHSAKIFLGDVRSLPLLEAFHASLEAYRASDSLPACQFEQRVQKRIALEQ
                     ELVIDPAFFQALKERLPRITRVQIRPKRGRTWNEFTKFRYDVTLLLDHDSSTPSPPPE
                     LKDWRQQRMSRRVLLDDLSNNRPGMLGFTRIPNARVARELELLDRLKNGYETVALDLL
                     KHQPDEAYGDSLDPE"
     gene            complement(4003..7221)
                     /locus_tag="VFQ79_13955"
     CDS             complement(4003..7221)
                     /locus_tag="VFQ79_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU0140821.1"
                     /translation="MMTASARSDEIEDIYELSPLQQGILFHTLYDPGSWIYFEQVSFP
                     VHGRLNRHAFRQAWRRVVERHVVLRTSFYWDDLTKPLQVVHRQVETPFKEKDWRGKTS
                     EEQAALLDNFLRTDRDAGFDLSQPPAFRLALFELTDVTFQCVFSFSHLLMDGWSLQTV
                     FREVTSAYASYSLGEEPQFPPTRPYADYIAWLQEQDTSAAETFWKRTLADFTFTASTG
                     IERPGTRPGQQGRRYGEHWALFSAPLTAALQAFAVQHRLTLSSLMHGAWALLLGRYSD
                     KDEVMFGSVVSGRPPELAGVESMVGLFINTIPVPARIRRDLSAAQWLEEFQTAQWESR
                     NYQYSSLVQIQSWSAVPAGVPLFNSLLAFENYPVSVPGEAAHQQGEVRHFQRTNYPLT
                     LAVVPGSELQILFSYDQDHFTDDAIRRMAGHFQTMVEGIVCNAATPVHSLPLLTPEER
                     RGLLEGNNTKTKYPRAECIHRLFEEQAARNPQAIAIDDDEEPLSYGELNRRANRLAWH
                     LISLGAGPEQIVGISTSEPSRMVAGILGILKTGAGYLPLDSDDPRERLALMIADAEAR
                     LIVTDNRTADALPANSVRLVRLETLWGDATQPDPGNPPHHSGGESIAYAMYTSGSTGT
                     PKAVAIPHRAISRLLLNTNYVRLTASDRMALQSNCSFDAATFEIWGALLHGGCLVPVK
                     KNVTLSPWLLASKIREKGISVMFVTTALFNRLASEMPSLFGPLRYLLVGGEPAEAKYF
                     RSVLEAGPPGCLLNMYGPTESTTFAVGYAAARVDEAAASVPIGRPISNTKTYLLDHNL
                     QLVPVGVTGHLYLGGDGLARGYLNRPELTAEKFIPDPFSGEAGARLYKTGDLARLLPD
                     GNIEFAGRIDDQIKIRGFRVEPGEIEAALASHSAVRQPVVVARREEQSGTLLVAYVVP
                     QPEAAPSAMELRRFLKTRLPEYMIPSAFVFIDKVPLSPNQKVDRNALFRLGEDHPAPL
                     ESHVPPRNRTEEQLAAIWADVLGVAKVGINDSFFELGGHSLLATQLISRIRETFEVEL
                     PFAAFFESPTVAGLAGLIETGRWTAQSAQPAAVLNEYEQGEL"
     gene            complement(7259..>7743)
                     /locus_tag="VFQ79_13960"
     CDS             complement(7259..>7743)
                     /locus_tag="VFQ79_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140822.1"
                     /translation="ANARTDVEVEEALRALEGNCQEDTVAWTMHLLGNCCLKLRAFIS
                     AWRDEHLHMPRNNLGGESTKSLTGSPDAVKAVMHMRDGAIAKDPMAPLARARGLASEL
                     PRAGSQKLTSYLDSDGSLDRRLLAVTGQITQRRFSDVQERLGFFANRCPFVPPSRRKA
                     "
BASE COUNT         1594 a         2305 c         2293 g         1551 t
ORIGIN      
        1 cttccgggtc gaggctatcg ccgtacgcct catccggctg gtgctttagc agatccagcg
       61 ctacggtttc gtagccgttc ttcagccggt ccagcagttc gagctctctc gcgacgcggg
      121 cgtttgggat acgggtgaac cccagcatcc cgggccggtt gttgctgaga tcatccaaca
      181 aaacccgccg cgacatccgt tgctgccgcc aatcctttaa ttcgggcggc ggtgatggag
      241 tggaactatc gtgatccaaa agaagagtga cgtcgtagcg gaacttggtg aattcgttcc
      301 aggtgcgccc gcgtttgggc ctgatctgaa cgcgagtaat acggggcagc cgctccttca
      361 gtgcctggaa aaaggccggg tcgatgacca gttcctgttc aagagcaatg cgcttctgca
      421 cgcgctgttc gaattggcag gctggcaagg aatcggaggc gcgatacgcc tccagtgacg
      481 catgaaaagc ctccaacaga gggaggcttc tcacgtcacc gagaaaaatc ttcgcgctgt
      541 ggttcgccgc cgctacggca ccttccaaca cgcgcagcag gtagtccatg ctggggaagt
      601 actgaatcac cgaatgaaga agaaccagat cgaagctttc ttctttcaga aacgaccagt
      661 cgtctgcatt gctttgaaac aggcgtatgt ttttcatcgc cgctgcatgc cgctctatgt
      721 aattcaaggc ggctgccgaa aagtcggttc cgaaatactc gtcgcagtgg ggcgcgagcc
      781 ggaagagaag aagtcccgct ccgcacccga cttccaaaac ccggcgcggc ttaagagaca
      841 gaacatgatc gaccgcttgc cccacccatt cgcggacttc acccgctgga atctgccggc
      901 cggtgtagct gctgttgaag cccgagatgt taaacgttgg gtcctgctcg ccgccggcct
      961 gaatgtacgt ctcatcccat acggcctgcc actcgggaac ccgatcgcca ctccaggact
     1021 cgttgtcctg ctgtagtccg gcgcctgtgt aacgtggatc ttgaacgatg tatgccacca
     1081 ggcgctgttc acctgtagcg tcggcgcgag catcgaccac ggccgattgc acgcctggat
     1141 gttggagcag cgccgcttca atctctccca actcgatgcg aaagcctctc agtttgatct
     1201 gctggtccat gcgggccagg tattcaagat tgccgtcggg gagatatcga acgagatcgc
     1261 ctgttttgta caatctctgc gctgcaccct cgaacgggtt tggaacgaat ttcgcgccgg
     1321 tcaaatcggg ccggttcaaa taacctcttg ccagccccac tccacccacg tgtaactctc
     1381 ccgggacgga aatcggaacg ggctgcccat tggaatcgag cacgtaaatc tgcatgttct
     1441 gaatcggccg gccgatgggg acctgtcgaa catttccgtt ttgctccgcg gggatatacc
     1501 acgttgcatc ccagaattcc gaagtgccgt agaggttata aagcgtggcg tgtggcatct
     1561 gcctgcggaa aagctcaaac aggtccgccg gaagcgcttc gccactcgac acccagaaac
     1621 ggagcgctgg aagccggtgc tgaagatcgg gaacggtatc cagcatcgcg cgaaggagcg
     1681 agggcacaac ccagatccgg gtcacgttgc gaccggcgag ttcctccacc agcgcaaagg
     1741 ggtccttcaa cacggcatcc ggaataattg ctgcgggcac tccctgcaac agtcctccaa
     1801 aaaactccca gatagagtcc acgaagctga tagcagtctt ctggctgaga acttcctcgc
     1861 gagtgaacgg ataatcccgc cacatccagt ccagccgatt caggatttgc ctgtgctcta
     1921 ccgcaacccc cttcggcctg ccggtcgatc ccgaggtgta gatcaggtac gccatatcgt
     1981 ccggaccgac ggtgcaagcc gggttatcgc cgctgtgacc ggctatcgct ctggcatcgt
     2041 cgtcaaggca gatagtgcgg ttgggaactt ctgcgagggc cggcaacaag gcacgttttg
     2101 tgatcagggc gccggcgcgc gaatccttca gcatgaacgc cagacgctcc cgcggatagg
     2161 tcgggtctag cggcagatag acacatccgg ctttgaagat tgcgagcagc ccgacgacaa
     2221 aatcgagtga gcgctccagg cagatgccaa ccgtcgatcc ggcttcaacc cccatgctct
     2281 tcaggtagtg cgccgtctgg ttggcgcgcc ggttcaaatc ttcgtaagtg agccgctgat
     2341 cgccacaaag gaacgcgctc gccgttggag ccgccgctac ttgtgtttca aactgttcga
     2401 cgaggctgcg atcgcaggga tatgcgcggt ccgtttgatt ccagtcgatg agcagttgcc
     2461 ggcgttccgc ctgcgtcaga agaggcagag ctgcaagccg ctggtccggg ttggcggcga
     2521 tcccttccag caaagtttca taatgcccgg ccatgcgctc gatagttccc gcatcgaaca
     2581 ggtcggtgtt gtactcaaac tgaacatgga tgcccgaggg tgtttcgaac gtgtgcaggg
     2641 caaggtcgaa gatagcggtt ccacgctcca cagcgaggtt gggcgacccg ctatccgagt
     2701 cctgcaagga tacggttggg gcattaaaaa gttggaatac cacctgaaat aacgggttgc
     2761 ggctcaggtc ccgatcggga gcgagttcct ccaccagctt ggtgaaaggc acgtcctggt
     2821 gggcatacgc tccgagcgcc gcttcgcgta cgcgccgcaa cagctctcga aattcaggat
     2881 cgccgccgca gtccgtccgg aataccagtg tgttcacgaa aaagccgata agcttctcaa
     2941 tctcgccgcg gttccggttg gcgatatagg agcccactac gatgtcatcc tgtccggtgt
     3001 atcggtacag caaggcctga aacgccgcca gcagcaccat aaacagcgtg gcgccttcct
     3061 tgtggcacag cgctttcagc ggcgcggtca gcgcggagga caatgtgacg ctgtggtatg
     3121 cgccgcggaa cgtctgcatg gcgggccggg ggcggtctgt cggcaactga agcgtgggga
     3181 tgtcctgaag ctgctgcttc cagtagttca actggcgctc caggacggcc ccacgcaacc
     3241 attggcgctg ccaggcggcg aaatcgccgt attgaatttc caggggcggc agcggcgaag
     3301 gtctgcccag aagaaacgcc ccatacagag tattcaactc ctggaaaaat acgcccatgg
     3361 accatccgtc gctcacgata tggtgcatgg ttagaaggaa gacatagtcg ctgtaaccca
     3421 gccaaacgag agtcgttcgc aaaagcggcc cacaactcaa atcgaacggt tttctcgatt
     3481 cttctgtcgc gatgcgcagc gcctcagctt ctctttcagc cggtgcgaga tgccggagat
     3541 cgatgacggg cagcggcagt tccaacgatg gagcaatcac ctgcgccggc tcatcgttga
     3601 tcagttggaa cgttgtgcga agcgattcgt ggcgccgcac aatctcgttg aggctgcgct
     3661 caagaatacc tgggtcgatt ggcgtcttga gccggatggc gctgtcgata ttaaagaagg
     3721 aatttccggg cgccagttga tctacaaacc agatccgctg ctgggagaag gagaccggca
     3781 acccggacgg tgtgcgcgga accgggacga tggccggcgc caaagacggt tggccggata
     3841 acgcctcgcg caggtgagcg atcaactccg ccttccgctc cgccatgtcc gccagcagcg
     3901 tgggagtcaa cactcccttt gccgctttat agcggaggcg gtcgccttcc agccacagct
     3961 tgacgtcgag gctgcgcaga tagctcagca gttcggatgc ggtcatagct ctccttgttc
     4021 gtattcgttc agaacggcag cgggctgggc actctgtgcc gtccatcgcc ccgtctcgat
     4081 aaggccggca agcccggcca ccgtcggcga ttcgaagaat gcagcaaacg gaagctcgac
     4141 ctcgaaagtc tctctaatgc gggagatcag ttgcgtggcc aacagggagt ggcctccgag
     4201 ctcgaagaag ctgtcattga tgcctacttt cgcaaccccc agtacatcgg cccagatggc
     4261 ggcgagttgt tcctccgtcc ggttccttgg cggcacatgc gattcgagcg gcgccggatg
     4321 gtcttcacca agacggaaaa gcgcattgcg gtctaccttc tgattcggcg acagcggtac
     4381 cttgtcgatg aagacgaatg ccgaagggat catgtattcc ggaagccgcg tcttgagaaa
     4441 gcgccgcaac tccatggcgg aaggcgccgc ctccggctgg ggaactacat aagccacaag
     4501 cagcgtgccg ctttgctcct ctcttcgcgc aacaactact ggctgccgga ccgccgaatg
     4561 actggcgaga gcggcttcta tttcgcccgg ctcgacgcgg aatccgcgaa ttttgatttg
     4621 atcgtcgatc cggcctgcga attcgatgtt cccgtccggg agaagccgcg ccagatctcc
     4681 tgttttgtag aggcgcgcgc cggcttcacc gctgaacgga tctggtataa acttctccgc
     4741 tgtcagctcg ggccggttca gatagccgcg cgccagcccg tcccctccca gataaagatg
     4801 gccggtaaca cccaccggca cgagttgcag attgtggtcc agcagatagg ttttcgtatt
     4861 ggagatgggt cggccgatgg gtacgctcgc cgccgcttcg tcaacccgcg ccgccgcata
     4921 gcccaccgcg aaggttgtgc tttccgtggg cccgtacatg ttcaggagac agcctggagg
     4981 tccggcttcc aaaacagagc gaaagtattt tgcttcggcc ggttcaccgc ccaccagtaa
     5041 gtaacgcagg gggccgaaca gcgacggcat ctcgctcgcc agccggttga acaacgccgt
     5101 cgtcacgaac atgacgctga ttcctttttc ccggatcttc gatgccagca gccagggcga
     5161 cagagtgacg ttctttttca ccggtaccag gcagcccccg tgcaacagcg cgccccaaat
     5221 ctcgaatgta gcggcatcaa acgagcaatt cgactgcagc gccatacggt ccgaagccgt
     5281 cagccggacg tagttggtgt tgagcaacag ccggctgatg gcccggtgcg gaatcgccac
     5341 tgcttttggc gtgccggtcg atccggaagt gtacatagcg taggcgatgc tctcgcctcc
     5401 ggaatggtgg ggaggattgc cggggtcagg ctgcgtggcg tctccccaaa gcgtctccag
     5461 gcgaaccagc cggacactat tggcagggag cgcgtctgcc gtgcgattat ccgtgacgat
     5521 gaggcgagcc tcggcatccg caatcatgag ggccagccgt tcccgcggat catcggagtc
     5581 cagcggcaaa tatccagccc cggtcttgag gattccgaga atgccggcga ccattcgcga
     5641 gggttccgat gtcgagatgc ccacgatctg ttccgggccg gcgcccagcg agatcaggtg
     5701 ccaggccagc cggtttgctc tccggttcag ctcaccgtag ctgagaggct cttcgtcgtc
     5761 atcgatcgcg atcgcttgcg gattgcgcgc ggcctgctct tcaaacaacc gatggatgca
     5821 ttccgcgcgg ggatactttg tcttcgtgtt attcccttcg agcaatcctc gccgctcttc
     5881 gggcgtcagc aaaggaaggg aatgcaccgg cgtggccgca ttgcaaacaa tgccctccac
     5941 catcgtttgg aaatggccgg ccattctccg gatcgcatca tcggtgaaat ggtcctgatc
     6001 gtaagagaac agaatctgca attcggaacc gggaacgact gccagcgtta gcgggtagtt
     6061 cgttctctga aaatgccgga cttccccttg ctggtgcgcc gcctcccccg gaacagacac
     6121 gggatagttt tcaaaggcca gaaggctgtt gaacagggga acgcccgcag gcaccgcgct
     6181 ccaggattgg atctgtacaa gggaactgta ttggtagttc cgggattccc actgggccgt
     6241 ttggaattct tccagccact gagcggcgga aaggtctctc cggattcgcg ccggcaccgg
     6301 tatggtattg atgaacagcc ccaccatcga ctcgaccccc gcaagttcgg gaggccttcc
     6361 tgagacgacg cttccgaaca tcacttcgtc tttgtcgctg tagcggccaa ggaggagtgc
     6421 ccacgcgcca tgcatcaagc tgctgagagt gagacgatgc tggacggcga atgcctgaag
     6481 tgcggcagtc aatggcgcgg aaaagagcgc ccaatgctct ccgtaccgcc ggccttgttg
     6541 tccgggcctg gttccgggcc gttcgatgcc ggtcgacgcc gtgaaagtga aatccgccag
     6601 agtccgtttc cagaacgttt ccgccgcgga agtatcctgt tcctgcagcc acgcaatgta
     6661 atctgcgtag ggacgagttg gagggaattg cggttcttct ccgagcgagt aggatgcata
     6721 agcggaagtg acttcccgaa agacagtctg caagctccac ccatccatga gcaggtggct
     6781 gaagctgaac acacactgga acgtgacgtc cgtcaattcg aacagggcca ggcggaaggc
     6841 cggcggctgc gacagatcga agccggcgtc ccggtccgtg cgtagaaaat tgtccagcag
     6901 cgccgcttgc tcctcgctgg ttttcccgcg ccaatccttt tctttgaaag gcgtttcgac
     6961 ctgccggtgc accacctgga gtggcttggt aaggtcgtcc cagtagaaag atgtgcgcaa
     7021 caccacgtgt ctttcgacga ctcgcctcca agcctgacga aaagcatgcc ggttgagtct
     7081 cccatgaacc ggaaaactga cctgctcgaa atagatccag gaacccggat catacaaggt
     7141 atgaaacaga atgccctgct gaagagggga aagctcgtag atgtcttcga tctcatcaga
     7201 ccgggctgaa gctgtcatca tcgtggaggc ctcggggaag gaattcgtac cgcgatcgtc
     7261 acgcctttct ccgtgacggc ggaacgaacg ggcaccggtt cgcaaaaaat cccaaacgct
     7321 cctgtacatc gctgaagcgc cgctgcgtaa tctgtccggt gaccgctaga agccggcggt
     7381 ccaacgagcc gtccgaatcc agataggagg tcagtttttg actgccggcc cgcggaagtt
     7441 cgctcgccag gccacgagcc cgcgcaagcg gcgccatcgg atctttggcg atggcgccat
     7501 cgcgcatatg cattaccgct ttcaccgcat cgggagagcc ggtcaacgat ttcgtcgact
     7561 cgccgccaag attgttccgc ggcatgtgga gatgctcgtc gcgccaggcg ctaataaagg
     7621 cgcgcaattt caggcagcaa ttgcccaaca gatgcatggt ccaggccacc gtatcttcct
     7681 ggcagttgcc ctccagggcg cgaagcgcct cttccacttc cacgtcggtg cgggcgttgg
     7741 cgg
//
LOCUS       DASTMK010000324        33843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_367563, whole genome shotgun sequence.
ACCESSION   DASTMK010000324 DASTMK010000000
VERSION     DASTMK010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 33843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33843
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367563"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..554)
                     /locus_tag="VFQ79_13965"
     CDS             complement(66..554)
                     /locus_tag="VFQ79_13965"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEU0140823.1"
                     /translation="MEPHIEPQKEHQWLQKLAGEWTFEVEATMAPGQPPVKSTGTEVV
                     RPLGGFWILAEGHGEMPGGGPATTIVTLGYDPQKNRFVGTFIGSMMSNLWVYDGELDA
                     AERVLTLDAEGPHMAVKGKLAKYKDIIEIKSDDHRILRSEMLGDDGKWQEIMTMHYRR
                     KR"
     gene            complement(558..944)
                     /locus_tag="VFQ79_13970"
     CDS             complement(558..944)
                     /locus_tag="VFQ79_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0140824.1"
                     /translation="MLMIYTDEQAMDEAERQQCYVESAQLAQELDAKGQYLAAAPLHP
                     TSTATTVRVRDGKRQVTDGPFAETREQFGGYFLIEAGNLDEAINIAGRIPAARAGTVE
                     IRPVIDVPGLPGDRSVQPVDAINDRR"
     gene            943..2346
                     /locus_tag="VFQ79_13975"
     CDS             943..2346
                     /locus_tag="VFQ79_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0140825.1"
                     /translation="MYFTRFPPSGESNGRRRNRQRTGEFFYHFYMRWDADKAVDEVYR
                     SDWGRIVATLIRITGDFDAAEETAQEAFTAAVDQWRAGGVPEFPRAWIIQTARHKAID
                     RIRRRVVFTEKLKTYAASIASQTSAEPDLDTTEIPDDRLRLIFTCCHPALAFDAQVAL
                     TLRALGGLETDEIARAFLVPPATMAQRLVRAKRKIRDAGIPYAVPGTNEMPARLDAVL
                     TVIYLIFNEGYAATRGGPIVRTDLCAEAIRLGRLVRTLMAPQPPAEVTGLLALMLLHD
                     SRREARLNEAGDLVVLEEQDRGRRDRQQIEEALPLVEEAFRNGAGPLAVQAAIAAVHC
                     RAARPEDTDWPRILRLYDLLERLQPSPIVSLNRSVAVAMVDGPQPALALIDALAAKGD
                     LDTYHLLHSARADLLRRLGSLEEAAKSYERALALVTNDSERRFLERRLREVQSAKVRR
                     AGGGGSAPVPPFKANDG"
     gene            2479..2907
                     /locus_tag="VFQ79_13980"
     CDS             2479..2907
                     /locus_tag="VFQ79_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140826.1"
                     /translation="MRESLARRKSGRKGKTHPDAYSPERTLGHARLAWENGWLVIDAR
                     VYDQLVAMQVPLAEARTAIDAALREISAADVKPPDFAFDPPGQGFVWHSKYFGCEMYL
                     KVRLEDARPRCKLYSLHQALHGITKKRPRKARTKGSRMKS"
     gene            2904..3422
                     /locus_tag="VFQ79_13985"
     CDS             2904..3422
                     /locus_tag="VFQ79_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II TA system antitoxin MqsA family protein"
                     /protein_id="HEU0140827.1"
                     /translation="MKCFECGAGNVERRTIHVRKELRGVDLTFLARVDVCDKCGSWEI
                     PLEEAGSFGAKLDKAYRKKLGLLSAEEIRQARRRLSMNQREFATYIGVGAASIKRWEL
                     GALQDKSSDEVIRLKTDAEYACKNLEFIRRQQGVQTMGHSPRTVRSSNSASSCRPQAG
                     DGGRTSGRREGI"
     gene            complement(3488..3979)
                     /locus_tag="VFQ79_13990"
     CDS             complement(3488..3979)
                     /locus_tag="VFQ79_13990"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0140828.1"
                     /translation="MRTELNIAGVLPALLNELIDGAAPEAAWVLNPRDPGMLKSLDKL
                     TAEEASAIPPGGGASIAAHVDHLCYGLQLLNRWSQGENPFAGADYTASWRRTKVSEEE
                     WASRREILRREAYLWREAIQCPRDVSEFELTGMVASVVHLAYHLGAIRQMNRSIRGPS
                     AED"
     gene            complement(3987..4376)
                     /locus_tag="VFQ79_13995"
     CDS             complement(3987..4376)
                     /locus_tag="VFQ79_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU0140829.1"
                     /translation="MIGQLIEQHRRMEVSLDALLALLGCTPQARTELKSRFQDAVSIA
                     LAHYDFEDRGLFDSLRGEMPAFIAKLKAQHDEVRELSQAVTQLLHAGAMEERERSDLL
                     LLCRRFVAMAQHNIIEEERDFFPLLAR"
     gene            complement(4376..4618)
                     /locus_tag="VFQ79_14000"
     CDS             complement(4376..4618)
                     /locus_tag="VFQ79_14000"
                     /inference="COORDINATES: protein motif:HMM:NF042796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6755 family protein"
                     /protein_id="HEU0140830.1"
                     /translation="MAGQRMIPQQRRGLTAIDGVMVLIVILLIVQMWLLAATLDNFLA
                     GHHETVLPGAILSGLIFAACVALYLFVRRLDRKIRH"
     gene            complement(4618..5181)
                     /locus_tag="VFQ79_14005"
     CDS             complement(4618..5181)
                     /locus_tag="VFQ79_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0140831.1"
                     /translation="MKLSKPTSTENGAGGAGFNARPSPPEPLWRDEFSIFSADEKYVY
                     RRQFTKFLVLTSFGMFVGNLWILVRSWFYEEPSYPMRAIARAGEIPVGGVKIFQYPGE
                     HDNCILVRTGQDEYVAYSQKCTHLSCAVYYAREANRLECPCHEGYFSIRDGSVLQGPP
                     PRPLPRVVLDRRGDELIATGIEMKPEA"
     gene            complement(5153..5755)
                     /locus_tag="VFQ79_14010"
     CDS             complement(5153..5755)
                     /locus_tag="VFQ79_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0140832.1"
                     /translation="MFGYEFYIDPERCIGCMSCVNACAECETHRGHSMINVDFIDRKN
                     TIATVPMICMHCDDPTCAEVCPADAIKKGEDGVVQSSLKPRCIACSNCVLACPFGVPK
                     MIVDFEQMMKCDMCYDRTSVGKRPMCATVCPSQALAYITAEEAAAKRRETPVNEFQFG
                     NETVRTRVFMMLPPEQEQLSIDVADYMWEQVDEIVQAYEY"
     gene            complement(5771..7981)
                     /locus_tag="VFQ79_14015"
     CDS             complement(5771..7981)
                     /locus_tag="VFQ79_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEU0140833.1"
                     /translation="MAKLPAPAEELAEKYGPHLKFIPPGGWKDQSRTAERLVKTHCCF
                     CGQQCGIKLKVRDNKVIGFDPWEEFPFNRGMLCPKGVKRYMQNEHPDRLLDPFIRGDA
                     GFRTATWDEALDLTVRRIQEVQAKYGPDAVAVYGGASLTTEKAYLLGKLARVALKTRN
                     IDYNGRLCMVSAGVAYKLAFGIDRAPNPWSDIPKAQVVFVTGANVAECAPITTNYIWQ
                     CRDNGGKLIVADPRLTPITRNADLYLPVRPGTDLALLLGMLHVIFRDGLAKREFLEQH
                     TTGWEAVEAYARDWNPDKAASISGVPPASIEKAAHMIGEADRAMMMHARGLEHQSKGV
                     ENCLAVINIALACGHIGREGAGCVMITGQGNGQGGREHGQKCDQLPGQRSITDPEARR
                     HVAKVWGVDPDELPGPGLSAVEIMEAIHRGEIKALLSICFNPLVSLPEGEYTREALER
                     LEFFGVIDFFLSETAQHADVVLAGSLHEEEEGIVCSAEGRVIHINKAVDPPGNARSDI
                     RIICDIAARLGKGNYFPFVESRQIMDELREASRGGVADYYGITYEKIDEQMGVFWPCP
                     DLDHPGTPRLFEGARFYHPDGKAHLQATEFRPGGDPVDEEFPVYLTTGRVVSQYLSGT
                     QTRRIGALVDQYPEPRLEIHPRLAEKYGISDGDWVAVRTRRNEILLRAMVVKTIRPDT
                     VFIPYHWPRERSANRLTHRTLDPRSKIPEYKVSACQLRLEKKADPLPVMGQSRV"
     gene            complement(7974..10187)
                     /locus_tag="VFQ79_14020"
     CDS             complement(7974..10187)
                     /locus_tag="VFQ79_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0140834.1"
                     /translation="MTPGRQLTLGTLSFTICFAVWGLISAFAPRFRDLYGLSATQTAL
                     LVAVPVLLGSLARIPMGMLADGFGGRIVFTVLMLFVAVPAAVVPLAPSYTALLGVAFF
                     LGMAGSSFSIGVGFVSRWYPPQRQGTALGVYGLGNIGQSAAVFLGPVIAAHYGWQAVF
                     YSAAAIVIVWGVVFGSLARNAPVTTRPRGLGEMFRVLATRRLSWGLSAFYFLTFGGFV
                     AFSIYLPTLLRDEFQLSPADAGFRAAGFVVLATLLRPVGGWLSDQIGGARVLSGVFLG
                     LIPFALLLAWASMIPFTVGALGCAALMGVGNGAVFKLVPQYFPVETGTVTGLVGAMGG
                     LGGFFPPLLLGVFRDRLGVVWPSFVLLAGVAALMWWINHRVFLAREEAAELTLSPQYA
                     RTAEQLRAAAWACMVTGLLVAAIVVGSRNLQNFDPALVIYTFATIFATFGVTYHYMVW
                     LQKPPTQRFWRRGWQLFRERGMLRSLLRIGRYSGTHLAGQTFIRRRSKLRWWMHQFLF
                     WGCLLAVAITFPLVFGWIHFASHPDDQMTYITYLFGFPVGSFPIRTIISWLLFHGLDI
                     AAVLVLAGITLSLWRRMRDEQAQALQSFSMDFLPIILLFAISTTGLALTVSTSLLRGQ
                     AFAFLAILHAITVIGALLYLPFGKFFHIFQRPAQLGVKLYQEAGAEDEGAVCVRCGTR
                     FASRMHIDDLKTVLPQLGFDYRMNGEAGNWQHFCPACKRKTIAMAQLRLKEEHRG"
     gene            10281..11900
                     /locus_tag="VFQ79_14025"
     CDS             10281..11900
                     /locus_tag="VFQ79_14025"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEU0140835.1"
                     /translation="MSSPDDQLPPVWKAAFERERGFAKPALADPLPERRLSRLLAAFI
                     ASGLLFMVLPGTLIGVWNLLSISAQRESTSAPAAWIQAHGHAQLFGWVGTFIIGISMY
                     AVPKFRGAWIRSLPLAWAAFALWVSGVTLRWCAGITGQHAHWALPAAALAELAAVLLV
                     IYEITPRSASKRQQGAWNTLILSGFAGLAVLLAFQLLVVLRIEGAIVPAGANHILLNM
                     ALWVFCVPVVTGFSAKFLPSLLALRRTSDHAAYGGLALIVIALVGSLAGFEKFSSGLI
                     LAAVLLLAHWLGVFHRSTGRAKTIGMDARYPLFARAAFAWLVVSAILAIWAEKPGVLG
                     ASRHAFTVGFLATLIFSIGPRILPTFLNSRELWSKRLVFWALVLLSTGCAIRVTTEPL
                     AYAGVANYAWSLLPASAILELTAVLLFASNMAVTLLRPMPAWMDLKLVTAELKVYWLV
                     SSYPDTRRILINAGLGTIGRAPAIPRTLTLAEAAEADGVPAENMVHLLKAYFEPRLAK
                     AARANQSARKALSGGNLQCGPGRGADHAVRG"
     gene            complement(11841..12755)
                     /locus_tag="VFQ79_14030"
     CDS             complement(11841..12755)
                     /locus_tag="VFQ79_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEU0140836.1"
                     /translation="MLSNLQKRTAQAIVNIFETGSPWGDYGSVTVSRGDAGHLTYGRA
                     QTTLASGNLHRLIRSYCESPGARCARPLSAYLERLERCDLSLDTETVLHDHLREAGRD
                     LTMRCLQDRFFDEHFWVPACAAASSLGLSTALAASVVYDSFIHGSWARMRDETNRLGR
                     PETLGERRWVGNYVNTRRRWLAGHRNPILRLTTYRMDCFVDLTKKANWTLALPLRVRG
                     FELTRELLEGDPPEPVRTLRLANPPMSGADVRGMQEALNRLRVPCNVSGVFDRDTAAA
                     VKRFQKSRGLSADGVVGAATRAALKVAA"
     gene            complement(12846..13331)
                     /locus_tag="VFQ79_14035"
     CDS             complement(12846..13331)
                     /locus_tag="VFQ79_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEU0140837.1"
                     /translation="MFFKSFFPTLALVAALGLGFHSSVVGAAKKSSSNKSGTAKAQTA
                     KSTKSSVKSSKKASTVRRRAPRRRSSRASSRKSRGQQSPTPERYLEIQQALADKGFFR
                     DTPDGKWGPSSAEALKEFQQANNLPATGKLDALSIIVMGLGPKRDAPAPALTTIAGAH
                     E"
     gene            complement(13413..15005)
                     /locus_tag="VFQ79_14040"
     CDS             complement(13413..15005)
                     /locus_tag="VFQ79_14040"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU0140838.1"
                     /translation="MSFLSKMRQQKLLSLALLLFTLSAGILIGTLITTGVNAAKDQVA
                     APDATPLSIPSVQELSNEFTKLAKQTEPSVVNITTDYTRRERQTSRNRRPGIGEEDED
                     GMDLFRRFFQGPSPEMPQRPWQQEATGSGFIVDKNGYIMTNYHVIERADGIKVKMAHD
                     KTEYKAKMVGYDRETDLAVIKIDPARQLDAVRIGNSDGVQVGDWAVAIGSPFGLEATV
                     TAGIVSATGRDIAGAQQFQRFIQTDAAINPGNSGGPLLNIRGEVIGINTAIATQSGGY
                     QGIGFALPVNTAVRVYNQIIKTGRVTRGSIGVGWNKNDKPEVLKALGVDQGVLVESVQ
                     EGGPAEKAGVKPEDIIVAMNGKAIKDGDDLVSRVAETPVGTEVTITVDRAGKRMDRQL
                     VIADRSKVFANDPRFRDDNPPIEEKNEGTQAKFGIYVRNMTESERQTKKFEESRGVLV
                     TRVEPGSFAGEIGLRENDIIVSINRQPVSTVDDVKRIQTSLRGGDAVAFRVMRANPGL
                     RRGEGESPWVGFFVAGTLPGTP"
     gene            complement(15104..15991)
                     /locus_tag="VFQ79_14045"
     CDS             complement(15104..15991)
                     /locus_tag="VFQ79_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0140839.1"
                     /translation="MRQRVSHWRAELALVCITGIWGGTFVVVKDALDHVSTLLFLTLR
                     FSLAAAVLAILYRKHLYRFGKLPLRAGIIAGLLLFTGQFFQTQGLRYTTPSKSAFITG
                     LSIVLVPLLSGFVYRKAPGVFEVAGVAVATIGLALMTIEPAALIPGVGDLLTLICAFA
                     FGAHIVAIGYFSSSVNFEALSALQISTAAGASALTFWWAEVPTVQWTAGVLGAVAVTG
                     LLATALAFTVQAWAQRFTTATRVALIFALEPVFAAMTSYFLGRETITVPIVAGAVLIL
                     AGILLVELKPSAVPPHPSG"
     gene            complement(15991..17232)
                     /locus_tag="VFQ79_14050"
     CDS             complement(15991..17232)
                     /locus_tag="VFQ79_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0140840.1"
                     /translation="MAETAFIDTKSVSELAGERFVRECTQWFTREKQWINERHLELCR
                     IPAPTFLEEQRAEWMTAQFRALGCDSRIDRAGNVIAHAFGRKEGPYIALTAHLDTVLS
                     PRTAEDIRVSPDGKFLGPGVSDNGAGLAGLLAVTAALRSCAAVGDLQGSLLIVANVGE
                     EGEGNLSGMRYLCRQSSLAPKIKGFVVLDGPAVDHITNQALASRRFEVAINGPGGHSW
                     SDYGTGNPVHALCRAISMLTEYRLNGGTPGPRSTINFGTIDGGLSINSIPTVARAKVD
                     IRSESPEKIEELASLLTAAVERSLETENERSSGPRVSAKIREIGSRPGGRMDEAAQIL
                     RYTRAVDAHLSIRSHLDCASTDANIPLSMGIPAVSIGAGGQGGGAHTPSEWYHPEGRD
                     LGLRRILLLLLMLLKDLPEIA"
     gene            17351..18532
                     /locus_tag="VFQ79_14055"
     CDS             17351..18532
                     /locus_tag="VFQ79_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140841.1"
                     /translation="MANLTRRHALAGAANFIILKPETVFGSQANSAVSFGVIGTGGRG
                     VYVGTHMANDSRTRLTAICDVFPDRIDTAKTRMPGGDKAKVFRNYKDLLGDAGIDAVL
                     IATPVFLHPEHFEAAVNARKHIYCEKPAGADVAGVKRLIRAGARADKTKTIQFGYQQQ
                     FSPEYLRAMEILKTGKIGQLTMVNSFWVLGGTPPTGFKSPYPPEEQKLRHWGWWRDYS
                     GGPIVEQDCHGVDTMNWYAGAHPTKAVGRGGLRYPLAYGDWTSDHHNVVYYYPNKIEG
                     WLLSIKHTAGFRDVKEQLYGSQGMLETARTYFRWHGPTQTAPLKNADDLADRSLIEKA
                     ESKREITIDAVEAFFTSIVNRKPYNMAQSSGESTLTALMGRMAFETGREVTWDEMMKS
                     A"
     gene            18565..20316
                     /locus_tag="VFQ79_14060"
     CDS             18565..20316
                     /locus_tag="VFQ79_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0140842.1"
                     /translation="MARKRILSRERERADAQSFLTLRLNRRSFLAAAGGALLGPASLA
                     AARELTCDVAVIGGGMGGCAAALAALRNGMRVVMTEETDWVGGQLTSQAVPPDEHAWI
                     EMFGATALYRAYRNGVREYYRKHYPLTPEARARVHLNPGDGSVSRITHEPPVSLAVIE
                     AMLAPHISGGRLIVLLSHKPVAADVDGDSVRAVTVRSTGTAAEKTIRARYFVDATEQG
                     DLLPLSKTEYVTGFESRKQTGEPHAPEQAQPANIQAFTVCYAVDYLEGEDHTIDKPEE
                     YSFWRDYVPKLTPPWPGKLLGWHMSNPVTLKVRNVSFDPSADQNTGNALNLWIYRRIA
                     NRANFVPGAYRGSISLINWPQNDYWLGNLHEVSDEEAAHHLKRGKQLSLSLLYWMQTE
                     APRPDGKQGWKGLRLRHDITGTEDGLAKYPYIRESRRIQAEFTVVEQHVGTEARMQAT
                     GKNREELTAEEFPDSVGVGSYRIDLHPSSGGDNYIDVSSLPFQIPLGALIPKRVENLL
                     AASKNLGVTHITNGCYRLHPVEWNIGEAAGCAAAQAVRRNVPPRKIRSDRRLLEEFQS
                     AIRAQGIETAWPRISPR"
     gene            20324..21508
                     /locus_tag="VFQ79_14065"
     CDS             20324..21508
                     /locus_tag="VFQ79_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140843.1"
                     /translation="MDAAGTIRTPEPPPRVADVERIARSTDPVLRNLEITECYYQLAC
                     ACYAWTGQRANWCTFATWASRQAGRTIRGEDFLANLERHLNLRQWFTHPIKTIWRKIV
                     RAGIYNPETTVGRLAREIHSPFDAFEHASAAVARGNLKVFEEIGREFARFLAVCGADA
                     APDPARIAAFCEGLRPGDPPDGQRYLRQAFARYYQQQFEPDPSVRAQMMYLANLEIGF
                     HEQTRLQPEIREALDVPVRTAHDIGKQWLSILFPGAGAWKRTLEKPAAAVLGVIARAI
                     SRLSADVARRVITDAMMTLTVPGRVIALGRNLDVPADPSLAQVTLQDLETMLSNIEPG
                     PGGADDCGTDDWSNLRERMHFIAHLFRAFAAQESLFRAPFTSEQVTQFQSGLLPDGDL
                     CA"
     gene            complement(21515..22429)
                     /locus_tag="VFQ79_14070"
     CDS             complement(21515..22429)
                     /locus_tag="VFQ79_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140844.1"
                     /translation="MHSRAILACLLFTAGISETGDGARTPVYGTPTVIAHLENRDVNE
                     SSGIVASRRNPGLFWTHNDSGGAPLLYLFDRKGRGLGTWRVRGATAMDWEDIAIGPGP
                     VRGRSYLYIGDIGDNRRSRREVVVFRVEEPAAGRPGATSETQMSTALRFRYPDAPHDA
                     EALAVHPHTGDIYIIVKASGEETRTSVYSARAPHRTGIVTTFELLGDVNLPAESELVL
                     LIGRATGADISPDGRRVVICDYLRAYEAVLPANAGFDAIWRQPFTALSLGPRKQGEAV
                     CYRLDGRALLATSEGRPCPLIETVRNAR"
     gene            complement(22453..23430)
                     /locus_tag="VFQ79_14075"
     CDS             complement(22453..23430)
                     /locus_tag="VFQ79_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU0140845.1"
                     /translation="MQRVKALLVVVLFTLNAFVPAAWASDAKKDPDMIGERDIAKGVN
                     FYSIEKEMALGKQLAEEVERQSRVVDDPVISEYVNRVGQNLVRNSDAKVPFTIKVLDS
                     DEVNAFALPGGYLFVNTGLIERTENEAQLAGVLAHEIAHVAARHGTRQATRGRIINIA
                     TIPLIFMGGWAGYIANQAAGVAVPLGFLKFSRGFEEEADLYGIQYLYKTGYDPTAFVD
                     FFEKLQTLEKKKPGTLAKVFSSHPMTDDRIKDAQKYMSTGLDPREEYVVTTSEYEEVK
                     ARVEALDRRRVREKDPNRPTLRRRSSGTIDAGGEQKPDDRDERPTLKRQ"
     gene            23561..24787
                     /locus_tag="VFQ79_14080"
     CDS             23561..24787
                     /locus_tag="VFQ79_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU0140846.1"
                     /translation="MRSLLLCFFFVLVGCGVATSFEIKWVHLSSRNGDLPDPGGSNQQ
                     TALLAADFDKSGATAFIVGCRVKAPALVLYRRSGNSWTRSVIENEYLPIEAGGAAADI
                     DGDGDLDAVFGGDYQSNQLWWWENPHPRFNPETSWKRHVIKNSGANQHHDQIFADVKG
                     SGKPQLLFWNQRAKAILQAGIPTDPRNAAAWPLETLYSGGAGDNVENAAKYAEGVDAA
                     DVDGDGRVDLLAGNSWFYRDEVGRYVPVRIGSIGGRIKTGRFQRGKLPQVVIAPGDGS
                     GPLRFYWSSGNPRSAESWSGRTLLERDMVHGHTLDVGDIDGDGHLDIFAAEMAQWTRN
                     PQPDHPEAKAWILYGDGKGNFRNTVLVTGHGWHEGKLADVDGDGDLDIINKPYTWNTP
                     RIDVWLNNGTAPARRK"
     gene            complement(25033..25158)
                     /locus_tag="VFQ79_14085"
     CDS             complement(25033..25158)
                     /locus_tag="VFQ79_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEU0140847.1"
                     /translation="MTFLADENFPRPAVEALRRAGVDVVWIAEANPGASDDEVLP"
     gene            complement(25155..25388)
                     /locus_tag="VFQ79_14090"
     CDS             complement(25155..25388)
                     /locus_tag="VFQ79_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU0140848.1"
                     /translation="MDWRDHIGVDPQILVGKPIVKGTRISVELVIDLLAAGWTEQQVL
                     DSYPTLNANDVRACLAYASEILHSEKVFPLEPA"
     gene            complement(25416..26858)
                     /locus_tag="VFQ79_14095"
     CDS             complement(25416..26858)
                     /locus_tag="VFQ79_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0140849.1"
                     /translation="MRRILDELNRGLGRRDFMRAAAGGALAALQGGEPHLLHGASSPK
                     PAARADTLILLWMAGGMAQTETFDPKTYTPFESGMEARRVLSTFPSIRTAVDNIQISQ
                     GLERIAKVMDRATLIRSHRVGDLGFILHSRHQFHWHTGYEPPQSLAVPHMGAFVAKTL
                     GSRNPDVPAFIDIGQNLEIGGESDAVKAFHTAGFLGSEYGPFLIADPRDAASAVRPPG
                     HMTQERFRRRYENYKKLAQNSPVMKYGSDYQRESLTRSIENAHRLLTSPAAKAFDLSL
                     EPEKSFAVYNTGRFGLGCLLARRLTEAGTRFIEVTTEYIPFRYWDTHENGHTRAAGMK
                     QQIDGPVSQLVLDLEERGLLDRTIIVLASEFGRDMMTEGKPGKTVKEQVQQPDVLTDL
                     KHYGMHRHFTEASSVLLFGGGFKKGFLYGRTADERPCGIVENPITIQDLHATIYNAMG
                     IAPDTSYVVEKRPFYVTKDGKGKVVPALYA"
     gene            complement(26872..29208)
                     /locus_tag="VFQ79_14100"
     CDS             complement(26872..29208)
                     /locus_tag="VFQ79_14100"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEU0140850.1"
                     /translation="MLFLVLAMCQVRAQSSAADASFARDVQPIFNARCAACHTGSEAQ
                     AGLRLHTRAEMLKGGVSGPAIVAGSSANSLLVARITGEKQPLMPLAGKPLSAAEIDVI
                     RRWIDAGAPGPGAIEEPSWTPVLSPRRPDVPENTSFRNPIDRFMAAYFQKAGVAFPEA
                     VSGEVFLRRVYLDLWGLLPTPQQRLEFLNNTGPDSRERLIEKLLAAEEPYSGHWISFW
                     NDLLHNDEGVSYVGDRKSITPWLVDALQKNLPYDRFARALLNPTEDSDPEGFLLGVNW
                     RGDVNASQTAVMQAAQNSAQVFLAVNLKCNSCHDSFISKWKLADAYGLASFYSEKPLE
                     LVRCDVPTGKTAVARFLYPELGGVATDAPLKEKREVAARLFTSPENGRFTRTFVNRVW
                     KQLFGRGLVEPVDDLDAEPWNADLLDWLASSFAEHGYDIQYLLRAILNSRTYQLPAVD
                     SAADPADRKEKFVFRGPQYRRLTAEQFVDAISAMTGEWRILQPRKAGEGTYSREWRLK
                     ASSLTRTLGRPIRDSAVTERNTAPSTLQALEMANGETAATLLHRGALRMLGQLPTPPP
                     NRFDSGVFRSDAIHVDVEISGARELRLLLEDHDSYDPARVTAGWTGARLTGPEGEVPL
                     AELPSRPAFQTGTLKIKGHDAQPAVLAPLSSELVFDLSGRRFTRFQATVGVDESSQTS
                     DISPSVRTFVFTEEPDRMQLVRVAGAPPVPPPPALTSVDDAISRVYLHALGREPSTRE
                     RASARQFFGGGRSVKLNSDGLEDFLWAVVMLPEFQFIR"
     gene            29342..30085
                     /locus_tag="VFQ79_14105"
     CDS             29342..30085
                     /locus_tag="VFQ79_14105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0140851.1"
                     /translation="MTSLTRRYTRCVLPALAAGLFVALAGCSRPAEEPKKEEPAERPA
                     AEAPPAAEAPKEVPTPPAEAKPEAKKAETKPGVVPATYKVRLDTSKGPVLIEVQKAWA
                     PLGAEQFHKLVTSGYYDGARFFRIVPNFIVQFGLAADPKVTKKWDRNIKDDPVTRTNR
                     LGAVTFATAGPNTRTTQVFINLKSNQFLDDQGFAPFGQVVEGMDIVQQLYSGYGEAPD
                     QDAITNRGNAYLSAQFPKLDYIKKATIVE"
     gene            complement(30110..31939)
                     /locus_tag="VFQ79_14110"
     CDS             complement(30110..31939)
                     /locus_tag="VFQ79_14110"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0140852.1"
                     /translation="MRKLQVPLLVLVIASFLGAQRKSSEQLIQHPARGTRGAIGAGSR
                     FAADAGMRLFHAGGNAADAGVAAMFAAAVAEYSHFGFGGEAPILIRSKDGRVFSIAGV
                     GTMPKLASADFFRQRRLEAGEVEDLEPGGLKGMVPVAGLMPALVPGMVDAALVALGEF
                     GTRTFAEVIQPAIELADGMPMDETRAGTIARSRRFFNRWPTSKQVFLPEGRVTLPGEI
                     FRQPDLARTLRDMVKKEEEARASGASRKAAIDTVRDYFYRGGIARKIDAFSRANGGLL
                     RYEDMAAFRLKPEEPVSTDYRGVTVYKPGFWSQGPSLIEALNILEGLDLRAMGHNSAD
                     YIHVLTEALKLAYADRDTYYGDPAFVKVPSGRLLSKEYAAERRDAISPKASLEFRPGA
                     FPGIRALHPSESEIPRVRIDDALMARDTTCINTADRDGLVFSATPSGGWLPSVIAGDT
                     GIPLTSRAQSFLLIPGHPNELAPGKRPRVTLSPTLVTRNGRPYLALSTPGGDAQDQAL
                     LQLLLNVVEFGMNAEAAVEAPRFQTRHLVSSFDNHAMNPGELMLDERIQQGVFEELAS
                     RGHKIARRTRWSSGAAPSMIRILSNGVLETGADPYGYRVAWAW"
     gene            32001..32489
                     /locus_tag="VFQ79_14115"
     CDS             32001..32489
                     /locus_tag="VFQ79_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0140853.1"
                     /translation="MSDVNELLERFRRGPELLAVATTGAAGSELDFVPGPEKWSVRQI
                     VCHLADAELEGAVRFRRVIAEENPALIAYDQEAWARHLGYSRRKLSQALESFRRIRSE
                     NHELLKALPEEAFSRTGTHNERGVLSLLDLLKIYTEHAEQHARQIREVRDAYKTHRTS
                     NG"
     gene            32511..33143
                     /gene="thiE"
                     /locus_tag="VFQ79_14120"
     CDS             32511..33143
                     /gene="thiE"
                     /locus_tag="VFQ79_14120"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0140854.1"
                     /translation="MTLPRLYPIVDRDTLIRVQCPVQTAVEVLLDRGIRFLQFRVKGH
                     IGRTEFAEFECVAELCARAGAALIVNDRADLAMLLRAGVHVGQDDLSPKDVRRLVGEN
                     RLLGLSTHNEDQFRAGIAEPVDYLAFGPIFATTTKENPDPVVGLEMLRMLRGLTTKPL
                     VAIGGITRANAASVLEAGADSVAVIGDLYPAVFTLGEFRKRVNEWQQLVR"
     gene            33122..>33843
                     /locus_tag="VFQ79_14125"
     CDS             33122..>33843
                     /locus_tag="VFQ79_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0140855.1"
                     /translation="MATTRQVDTGFIKGLGLLDSTTLVIGSMIGSGIFIVSADIARQV
                     NSPGLLIIPWLVTAVLTMIAALTYGELAAAMPRAGGQYIYLREAYGPLFGFLYGWTLF
                     LVIQTGTVAAVAVAFSKFAGVFFPSISATNYVIRAGGFHVNTQQALAIGVIVLLTAVN
                     TRGIHTGAMVQNIFTFAKTAALLGLIVLGFLVGRNAAAIEVNFTDFWRGADWSWTVIR
                     VVGVAMVGALFASDAWNNVTFTA"
BASE COUNT         6647 a        10558 c        10031 g         6607 t
ORIGIN      
        1 aaagaccact cgctgacgca cgcggctccg cttctgagcc gtgaccgtga gggagcggtt
       61 ttttactacc gcttacggcg atagtgcatc gtcatgatct cctgccactt tccatcgtcg
      121 cccagcatct cggatcgcaa aatccggtgg tcgtcgcttt tgatctcgat gatgtcctta
      181 tacttcgcca gcttaccctt gacggccata tgcggtccct cggcgtccag cgtcaggacc
      241 cgctccgccg cgtccagttc gccgtcatac acccacaggt tgctcatcat cgatccgata
      301 aatgtgccca caaaccgatt cttctgcggg tcatagccga gcgtcacgat cgtcgtcgcc
      361 ggaccgccac cgggcatctc gccgtgccct tccgccagga tccagaagcc gcctagcggc
      421 cgcacgacct ccgttcccgt actctttacc ggcggctggc ccggcgccat tgtagcctcc
      481 acttcaaacg tccactcgcc ggcgagcttt tgtaaccact ggtgttcctt ctgtggttcg
      541 atatgtggtt ccatgtctca tctcctgtcg ttgatggcgt ccacgggctg gacagaccgg
      601 tccccgggta gacccggcac atcgatcacc gggcgaatct ccacggtgcc ggcgcgtgcg
      661 gcgggaattc ggccagcgat attaatggct tcatcgagat tcccggcttc gatcaggaaa
      721 tagccgccga actgctcgcg cgtctcggca aacggaccat cggtaacctg ccgcttgccg
      781 tcgcgcaccc gaacggtagt agccgtcgaa gtaggatgca gcggggcggc ggccaggtac
      841 tgccccttcg cgtcgagctc ttgtgcgagt tgagcggact ccacataaca ttgctgccgc
      901 tcggcctcgt ccatcgcttg ttcgtcggtg tagatcatca acatgtactt cacgcgtttt
      961 ccccccagcg gtgaatcgaa cggccggcgc cggaatcgac aacgcaccgg ggaatttttt
     1021 taccatttct atatgcgctg ggacgccgac aaagctgtgg acgaggttta tcgctccgat
     1081 tggggccgga ttgtcgctac gctgatccgg atcactggcg acttcgacgc agccgaggaa
     1141 acggcccagg aagcttttac ggccgctgtt gatcagtggc gggccggcgg agtaccggag
     1201 ttcccgcgcg cctggatcat ccagacggcg cggcacaagg ccatcgatcg catccggcgc
     1261 cgggtggtgt tcaccgagaa gctcaagacc tacgcggcgt ccatcgcatc ccagacttcc
     1321 gcggaacccg atttggacac aacggagatt ccggacgacc ggcttcgcct gattttcacc
     1381 tgctgtcatc cagcacttgc gtttgacgcc caggtcgcat tgacgctccg tgcgctaggc
     1441 ggcctcgaaa cggacgagat cgcacgtgcg tttctcgttc ccccggcgac gatggcgcag
     1501 cggctcgtac gagcgaagcg caagattcgc gacgcgggca ttccgtatgc ggtacccggc
     1561 acgaacgaaa tgccggcccg gctcgatgcg gtcctgacgg tgatctatct cattttcaac
     1621 gaaggttacg cggccactcg aggcggcccg atcgtgagaa cggatctttg tgcggaggcg
     1681 atccgtctcg ggcgcctcgt aagaacttta atggccccgc agcctccggc ggaagtaacc
     1741 ggccttctgg ctctcatgct gcttcacgat tcgcggcgcg aggcgcggct gaatgaagcc
     1801 ggcgacctcg tcgttctcga agagcaggat cgtgggcgcc gggaccggca gcagattgag
     1861 gaagcactgc cgctggtcga agaggcattt cgcaacggtg caggtcccct tgcggtgcaa
     1921 gcggccatcg cggccgtgca ttgccgcgcg gcgcggccgg aggatacgga ctggcctcgg
     1981 attcttcgac tctacgacct cctggaacgc cttcagccct ctccgatcgt gtcgctgaat
     2041 cgctcggtgg ccgtggccat ggtggacggc ccgcaacccg cgctcgccct catcgatgca
     2101 cttgccgcca agggcgacct cgacacctac catttactgc attccgcccg tgccgatttg
     2161 ctgcgccgcc tgggatccct ggaggaagcg gcaaaaagct acgagagagc gctcgcgctg
     2221 gtcaccaacg acagcgagcg ccgttttctg gagcgccggc ttcgtgaggt tcaatcggca
     2281 aaggtcaggc gggcaggcgg aggaggaagt gcgccagtgc cgccgtttaa ggcgaacgat
     2341 ggctaatcct gcgttcacac acccctggat ctcgtctcgg cgagcacagt ttatctatga
     2401 atcggcatcc gatcacatct tccggcagaa acggctcttg tcaaagttgg tatcaaatga
     2461 taccatcagg tgtgaggtat gagggagtcc ctggcacggc ggaagtccgg gcgaaagggc
     2521 aagacgcacc ccgacgcgta ctccccggaa cgaacgctcg gtcacgccag gttggcctgg
     2581 gaaaacggtt ggcttgttat tgacgcccgg gtctatgatc agttggtagc gatgcaagtc
     2641 ccactcgcgg aggcccgaac ggctatcgat gccgcgcttc gggaaatcag cgcagccgac
     2701 gtgaaacctc cggacttcgc attcgatccg ccgggacagg gctttgtatg gcattccaag
     2761 tatttcgggt gtgagatgta ccttaaagtc cgtctcgaag atgcgcgccc ccgttgcaag
     2821 ctgtattcgc tccaccaggc gttacacggc atcacaaaga aaaggcctcg gaaggctcgt
     2881 accaaaggtt caaggatgaa atcatgaagt gttttgaatg cggcgccgga aacgtcgaac
     2941 gacgtaccat tcatgtgaga aaggaactgc gcggggtaga cctcaccttt cttgctcgag
     3001 tcgatgtctg cgacaaatgt ggctcctggg agattccgct tgaagaagcc ggctccttcg
     3061 gcgcgaaact ggacaaagct taccggaaga aacttggcct cctctctgct gaggaaatcc
     3121 gccaagcccg ccggcgtctc agcatgaacc agcgcgagtt tgccacatac atcggtgtcg
     3181 gtgcagctag catcaagcgc tgggaactcg gcgcgcttca ggacaagagc agcgacgagg
     3241 tgattcgcct taagacagat gccgaatacg catgcaagaa cctcgaattc atacgccgcc
     3301 agcagggtgt gcagactatg ggccactcgc ctcggactgt ccgttcctcc aattccgctt
     3361 cttcgtgtcg tccgcaagcg ggagacggtg gaaggacctc cgggcggcgt gagggaatct
     3421 aagcgtccga agcagatgcg gcgcggagcg gtcaaaaccc gctcctgatc cagcaacacg
     3481 gccagtgcta atcctccgcc gaaggacccc ggatagaccg gttcatctgg cggatcgctc
     3541 ccaggtgata cgcgagatgg acgacgcttg ccaccatccc ggtcagttcg aattcgctga
     3601 cgtcgcgcgg acattgtatc gcttcccgcc acaagtaggc ttcacgtcga aggatctccc
     3661 gccgggacgc ccattcctct tcggacactt tcgtgcggcg ccagctcgca gtgtagtcgg
     3721 ccccggcgaa tggattttca ccctggctcc agcggttcag caactgtagc ccgtaacaca
     3781 aatgatccac atgagccgca atggatgcac cgccaccggg aggaatcgcc gatgcttcct
     3841 ccgcggttag cttgtcgagc gacttgagca tcccggggtc ccgaggattg agtacccaag
     3901 cggcctccgg cgcagctccg tcgatcagtt cattcagtag cgccggtagt acaccggcga
     3961 tgttcagctc agtcctcata aaacagctat cgcgccaaca gtggaaagaa gtcccgctct
     4021 tcctcaatga tattgtgctg cgccattgcc acaaaacggc ggcagagtaa cagcagatcc
     4081 gatcgttctc tctcctccat tgcacccgca tgcagcaatt gggtcacggc ctggcttaac
     4141 tcgcgcactt catcgtgctg ggctttcagt ttggcaataa acgcgggcat ttcgccgcgg
     4201 agagagtcga acaatccacg atcttcgaaa tcatagtgag ccagcgctat tgaaacggca
     4261 tcctggaagc ggctcttgag ttccgtccgg gcctggggcg tgcacccgag cagcgcaagc
     4321 agcgcgtcaa gcgagacctc catccgccgg tgctgttcga tcagttggcc gatcatcagt
     4381 gcctgatctt gcggtccagc cttcgcacaa acagatacag cgcgacgcag gcggcaaaga
     4441 tcaaccccga taggattgcg cccggcagca ccgtttcatg atgcccggca agaaagttat
     4501 ccagtgtcgc ggcaaggagc cacatctgca caatcagcag aatgacgatc aacaccatca
     4561 cgccgtcaat cgccgtcagc ccgcgcctct gttgtggaat catccgctgg cccgccatca
     4621 cgcctccggt ttcatctcga tgcctgtggc aatcagttca tcgccgcggc ggtccagcac
     4681 gaccctcggc agaggccggg gcggcggccc ctgaaggacg ctgccgtcgc gtatggaaaa
     4741 gtacccttca tggcaggggc actccagccg gttcgcttcg cgcgcgtaat aaaccgcgca
     4801 ggacagatgc gtgcatttct ggctgtatgc aacatactca tcctgcccgg ttcgaaccag
     4861 aatgcagtta tcgtgctcgc cggggtattg aaagatcttg acgccgccga ccggaatttc
     4921 ccccgcgcgc gcgatggcac gcatcggata cgacggctcc tcgtaaaacc aggagcgcac
     4981 cagaatccac aggttcccga caaacatgcc gaaactcgtc agcactagaa acttggtgaa
     5041 ctggcggcgg taaacatact tctcatcggc gctgaagatg gagaactcat cgcgccataa
     5101 cggctccggc ggcgaggggc gagcgttgaa cccggctccg ccggccccgt tttcagtact
     5161 cgtaggcttg gacaatttca tcaacctgtt cccacatgta atccgctacg tcgatgctca
     5221 actgttcctg ttccggaggc agcatcataa acaccctcgt ccggacggtc tcgttgccga
     5281 actggaattc gttcaccgga gtttcccgcc gtttggccgc ggcttcctcc gcagtgatgt
     5341 aggccagcgc ctggctcggg cagacggtag cgcacatcgg gcgtttcccg acggaagtcc
     5401 ggtcgtagca catgtcgcac ttcatcatct gctcgaaatc cacgatcatc ttcggcactc
     5461 cgaaaggaca ggccagcacg caattggagc acgcaataca acgaggcttc aaggaggact
     5521 gcacgacgcc gtcttctccc tttttgatcg cgtcggcggg gcagacctcg gcacaggtcg
     5581 ggtcgtcgca atgcatgcag atcatcggca ctgtggcgat ggtgttcttc cggtcgatga
     5641 aatccacgtt gatcatcgaa tggccgcggt gcgtctcgca ctccgcacac gcattcacgc
     5701 acgacatgca gccgatacac cgctccggat cgatatagaa ttcataaccg aacatgattc
     5761 cctctcgcct ctatacccgg ctctggccca tgaccggcag cggatcggcc ttcttctcca
     5821 gccggagctg gcaggccgaa accttgtatt ccgggatttt gctccgcggg tcgagcgtcc
     5881 ggtgcgtcag ccggttggcg cttcgttccc gcggccagtg atacggaatg aacaccgtgt
     5941 cgggccggat ggtctttacc accatggcac gcagcaaaat ctcgttgcgc cgcgtccgga
     6001 cggccaccca gtcgccgtcc gaaattccat acttctccgc caggcgcgga tggatctcaa
     6061 gcctcggctc cggatactgg tccaccagcg ctccaatacg ccgcgtctgc gttcccgaca
     6121 ggtactggct cacaacacgg ccggtagtga gatagaccgg aaactcttcg tccaccggat
     6181 cgccgcccgg ccggaactcc gtcgcctgca ggtgggcctt tccatcggga tgatagaaac
     6241 gcgcgccttc gaacaacctc ggcgtgccgg gatgatccag atccggacag ggccagaaca
     6301 cccccatttg ctcgtcgatc ttttcgtaag tgatgccgta gtagtcggcg acaccgcccc
     6361 gcgaggcttc ccgcaattcg tccatgatct ggcgcgactc gacgaacgga aagtagttgc
     6421 ccttgcccag acgtgccgcg atgtcgcaaa tgatccggat gtcgctccgc gcgttaccgg
     6481 gcgggtccac ggctttattg atgtggatca cacggccttc cgcgctgcac acaatgccct
     6541 cttcttcctc gtgcaggctg ccggccagca cgacgtccgc atgctgcgcc gtctccgaca
     6601 agaaaaagtc aatgacgccg aaaaactcga ggcgctcgag cgcctccctc gtgtattcgc
     6661 cctcgggaag cgataccagc gggttaaagc agatagacag cagcgccttg atttctcccc
     6721 ggtgaatagc ttccatgatt tcaaccgcac tcaaaccggg gccgggaagc tcgtccgggt
     6781 ccacacccca aaccttcgcg acatgccggc gcgcctcggg atccgtaatg gagcgttgtc
     6841 cgggcaactg gtcgcatttc tggccgtgct cgcggccgcc ttgaccgttg ccctgacccg
     6901 ttatcatgac gcaacccgcg ccttcccgcc cgatgtggcc acacgccagc gcgatgttga
     6961 tcacggccag gcaattctcg acgcctttcg actggtgctc caggccgcgc gcgtgcatca
     7021 tcatcgcacg gtctgcctca ccgatcatgt gcgcggcctt ctcgatggat gccggaggca
     7081 cgccgctgat cgaggctgcc ttgtccggat tccagtcgcg cgcgtacgcc tccaccgctt
     7141 cccatccggt ggtgtgttgc tccaggaact cgcgtttcgc caggccatcg cgaaagatga
     7201 catgaagcat cccaagcagc aatgccaggt cggtacccgg ccggaccggc agatacaggt
     7261 ccgcgttgcg cgtaatgggc gtcaggcgcg gatcggcaac gatcagcttg ccgccgttat
     7321 cgcggcactg ccaaatgtag ttcgtggtaa tgggagcgca ctcggcaaca ttggcgcccg
     7381 tcacgaacac cacctgtgcc ttcgggatgt cgctccaggg attcggcgcg cggtcgatac
     7441 cgaacgccag cttgtacgct acgcccgccg acaccatgca gagacggccg ttataatcga
     7501 tgttccgcgt cttcagcgcc acgcgcgcga gcttgccgag caggtaggcc ttttccgtgg
     7561 tgagcgacgc gcctccatag accgcgactg catcggggcc gtacttggcc tgcacctcct
     7621 ggattctgcg tactgtcagg tcgagcgctt cgtcccaggt cgcagtccgg aaaccggcat
     7681 cgccgcggat gaatggatcg agcaaccggt cgggatgctc gttctgcatg tagcgcttca
     7741 cccccttggg gcagagcatg ccgcggttga acgggaactc ctcccatggg tcgaagccga
     7801 tgactttgtt atcgcgtacc ttcagcttga tgccgcactg ctgcccacag aagcagcaat
     7861 gcgtcttcac cagccgctcc gcagtgcggg attgatcttt ccagccgccg ggaggaatga
     7921 acttcaggtg aggcccgtac ttctcagcca gttcctccgc gggcgcggga agtttagcca
     7981 cgatgctcct ccttgagccg taactgcgcc atcgcgatcg tttttcgttt gcaagccgga
     8041 cagaagtgct gccagttgcc cgcctctcca ttcatcctgt agtcgaagcc gagttgcggc
     8101 agcacggttt tcaggtcgtc tatatgcata cgcgatgcga atcgcgttcc gcatcgcaca
     8161 caaacggccc cttcgtcttc cgccccggcc tcctggtaga gcttcacgcc aagctgcgcc
     8221 ggtcgctgga agatatggaa gaactttccg aaaggcagat agagcagagc tccaatgacc
     8281 gtaatcgcgt gcaggatggc gaggaatgca aacgcctgcc cccttaacag cgacgtggaa
     8341 accgtcagcg ccaggcccgt cgtcgagatc gcgaaaagta ggatgatcgg caggaaatcc
     8401 atcgagaagg attgcagcgc ctgtgcctgt tcgtcgcgca tcctgcgcca caacgagagc
     8461 gtgatacccg ccagcaccag cacggcggcg atgtccagcc cgtgaaacag cagccaggat
     8521 attatcgttc gaatcgggaa ggaaccaact ggaaacccga aaagataggt tatgtaagtc
     8581 atttggtcgt ccggatggct cgcgaaatga atccatccga agaccagcgg aaacgtgatg
     8641 gctacggcca gcaggcatcc ccagaacagg aactggtgca tccaccagcg cagtttcgag
     8701 cgccgccgga tgaacgtctg cccagccagg tgcgtacccg aataccggcc gatgcgcagc
     8761 aggcttcgca gcatcccgcg ctcgcggaac aactgccatc ctcttcgcca gaacctttgc
     8821 gtgggcggct tctgaagcca gaccatgtag tgatacgtga cgccgaaggt ggcaaagatc
     8881 gtggcgaagg tgtagatcac aagagccgga tcgaaattct gcagattgcg ggagcctacg
     8941 acgattgccg caaccagcaa acccgtcacc atgcatgccc acgcggcggc gcgaagctgc
     9001 tccgctgttc gcgcatactg aggcgaaagg gtgagctccg ccgcttcctc tctggcaaga
     9061 aatacccgat ggttgatcca ccacatcagg gccgccactc cggccagcaa aacgaagctc
     9121 ggccacacca cgccaagacg atcgcggaac actccgagca gcaggggtgg aaagaatccg
     9181 ccgagtcctc ccatggcgcc caccaggccg gtgacggtgc cggtttcaac cggaaagtac
     9241 tgcggcacta atttgaaaac ggctccgttg cccacaccca ttaacgccgc acaaccgagc
     9301 gcaccgacgg tgaatggaat catggacgcc catgccagca gcagtgcgaa cgggatgagt
     9361 cccaggaata cgcccgatag tacccgtgct ccgccgatct gatccgacag ccaaccgcct
     9421 acgggtctca gcagggtcgc aagcacgacg aagccggcgg cgcgaaatcc ggcatcggcc
     9481 ggcgaaagct gaaactcgtc gcgcagcaaa gtcggcagat aaatggagaa ggcgacgaac
     9541 ccgccgaacg tcaggaaata gaacgccgag agcccccacg acagccgccg ggtagcgagc
     9601 accctgaaca tttcgccaag tccccggggc cgtgtggtga caggagcgtt gcgcgcgagg
     9661 ctcccaaaca cgacgcccca cacgatcacg atcgctgccg ccgaataaaa cacagcctgc
     9721 cagccatagt gcgcggcgat gacgggcccc aggaacacag cggcggattg accgatgttg
     9781 cccaacccat agacaccgag cgccgtgccc tgccgctgtg gtggatacca tctcgacacg
     9841 aacccgacgc caatcgaaaa ggacgagccc gccatcccca ggaagaaagc gacaccgaga
     9901 agcgctgtat agctcggcgc gagtggaacc accgcggcgg gcaccgcaac aaacagcatc
     9961 agcaccgtga agacgatgcg ccctccgaaa ccgtcggcca gcatgcccat cggaattctg
    10021 gcaagagatc cgagcaacac cggcacggct accagcaacg cggtttgtgt ggcggataat
    10081 ccgtagaggt cgcgaaagcg gggtgcgaag gcgctgatca ggccccagac ggcaaagcag
    10141 atggtgaaag acagagtccc aagtgttagc tgcctacctg gagtcataca ttgtagattg
    10201 ccacattgta gtgaatgtta cgcggtgact ccggtcactg atgcccctcg ccgcttgcgg
    10261 caagctggaa tcagaatccg atgagcagcc cggacgatca actcccacct gtctggaagg
    10321 cggcgttcga acgcgaacgc ggcttcgcca agccagccct ggcggacccg cttccggagc
    10381 gccgcctgtc acgcctgctt gccgccttca ttgcgtcggg ccttctgttc atggtgctgc
    10441 cgggtacgct cataggagtc tggaatcttc tcagcatcag cgcgcagcgc gagtccacgt
    10501 cggcgccggc ggcgtggatt caggcgcacg gacatgcaca gcttttcggt tgggtgggaa
    10561 cgttcatcat cggcatctcg atgtatgcgg tgcccaaatt tcgcggcgcc tggattcgtt
    10621 ctcttccttt ggcatgggcc gccttcgcgt tgtgggtgtc cggagtgacg ttgcggtggt
    10681 gcgcgggcat caccgggcag cacgcgcact gggcgcttcc cgccgctgcg cttgcggaac
    10741 tggcggcggt tctgctcgtt atttatgaaa tcacaccccg ctccgcttcc aaaagacaac
    10801 agggcgcgtg gaatacgttg atcttgtccg ggttcgcggg gctggccgtg ctgctcgctt
    10861 ttcagctctt ggtggttctg cggatcgagg gcgcgattgt tcccgccggg gccaatcaca
    10921 tcctgctgaa catggccctg tgggtgttct gtgttcccgt ggtgacgggg ttcagcgcga
    10981 aatttctccc cagcctgctg gcgctgcgca ggacaagcga ccatgcggca tatggcggtc
    11041 ttgcactgat cgtcatcgct ctcgtgggct cgctggcagg atttgagaaa ttctcttcgg
    11101 gattgatttt ggcggcggtt ctcctgcttg cgcactggtt gggggtcttc catcgaagca
    11161 ccggccgcgc caaaacgatc ggcatggacg cgcgctaccc tctattcgcg cgggccgctt
    11221 tcgcgtggct cgttgtgtcg gcgatcctgg cgatctgggc cgaaaaacca ggggttctgg
    11281 gcgcatcgcg gcacgctttc accgttggct ttctggcgac cctgatcttt tccatcggcc
    11341 cgagaatcct gcccacgttt ctgaacagcc gtgaactgtg gagcaaacgc ctggtgtttt
    11401 gggcgcttgt tctgctttca accggctgtg ccatccgcgt gacaacggag cccctggcgt
    11461 acgctggagt agcgaattac gcatggagtc ttcttccggc gtcggcgata ttggaactta
    11521 cggccgtact gctgttcgcc tcgaacatgg cagtaactct gttgaggccc atgcccgcct
    11581 ggatggatct gaagcttgtg accgccgaat tgaaagttta ctggctggtg tccagttatc
    11641 ccgatacccg ccgcatcctg attaacgccg gccttgggac catcgggcgc gcacccgcca
    11701 ttccgcgcac gctcactctc gccgaagccg cggaagcgga tggtgtgccg gcggaaaaca
    11761 tggttcatct gctgaaagcc tacttcgaac cacgccttgc gaaagctgct cgtgcgaatc
    11821 aaagcgcgcg gaaagcgctt tcaggcggca accttcagtg cggcccgggt cgcggcgccg
    11881 accacgccgt ccgcggataa tccccggctt ttctgaaacc gcttcaccgc cgcggcggtg
    11941 tcccggtcaa acacgccgct cacattgcac ggcacccgta accggttcaa agcctcctgc
    12001 atgccgcgca cgtccgcgcc cgacatcgga ggattcgcca gccgcagcgt gcggacgggc
    12061 tccggtggat cgccttcgag caactcgcgc gtcaactcga atccccggac gcgcagaggc
    12121 agggcgagcg tccagttggc tttctttgtg agatccacga aacaatccat gcgataggtc
    12181 gtcagcctga gaatcgggtt acggtgtccc gccagccatc gccgcctcgt gttcacgtag
    12241 ttccccaccc atcgccgctc gcccagcgtc tccggcctgc cgagccggtt cgtctcatcg
    12301 cgcatccggg cccagcttcc atgaataaag ctgtcgtaca ccaccgaagc cgccagggcg
    12361 gtagagagac ccagggagga cgccgccgcg caggccggaa cccagaaatg ctcatcgaag
    12421 aaacggtcct gcaaacagcg catcgtcagg tcgcgtcccg cttcacggag atgatcgtgc
    12481 aataccgtct cggtatcgag agagaggtca caccgttcca accgctccag gtacgcgctt
    12541 agaggcctcg cgcatcgagc gcccggggac tcgcaatagg aacggatcaa ccggtgcaaa
    12601 ttcccgctgg ccagtgtagt ttgagctcgt ccgtaagtaa gatggcccgc gtctccacgg
    12661 gacacggtca cactgccata gtcgccccac ggcgagccgg tttcgaatat gttgacaata
    12721 gcttgcgcgg tacgtttttg caggttgctg agcatacggt cttccggcca ccgattgtac
    12781 agtgcgccgc accgctgccc tgcgtcgctc ccagcgtttt gtgaaactta gcctttgtga
    12841 atcttctatt cgtgcgcgcc ggcgatggtg gtaagcgctg gcgccggggc gtcccgcttc
    12901 ggtccgagac ccatgacgat gattgacagc gcgtccagct tacccgttgc cggcaagttg
    12961 ttcgcctgtt gaaattcctt caacgcctcc gcggaggacg gtccccattt gccgtcgggg
    13021 gtgtcgcgga agaaaccctt atcggccaga gcctgctgga tttcgaggta gcgttcgggc
    13081 gtgggggatt gttgcccccg cgacttgcgg gaagaagccc gggacgatct tcttcgcggt
    13141 gcgcgccgcc gcacagtgga agctttcttg ctggatttga cggaggattt cgtactctta
    13201 gcggtttggg ctttcgctgt accgctcttg ttcgaagacg attttttcgc ggctcccacg
    13261 acggacgagt ggaagcccaa ccccagagcc gcgactagcg ccagtgtggg gaaaaaagac
    13321 ttaaaaaaca cgattcttct ttaatgtaac aaagaaaagg gacgcctttt caacaaaagc
    13381 gtccctttag agaaagaaaa atccccggcc ggttacggtg tgccgggcag agttcccgcc
    13441 acgaagaacc caacccacgg cgactcgccc tcgccgcggc gaaggcccgg gttcgcccgc
    13501 atgacgcgaa acgcgaccgc atcgccgccg cgcagcgacg tctgtatccg cttgacgtcg
    13561 tctacggtgc tgaccggctg gcggttgatg gaaacgatga tgtcgttctc ccgcagaccg
    13621 atttcgcccg cgaaggagcc cggctctact cgcgttacca gcacgccgcg gctttcttcg
    13681 aacttcttgg tctgccgttc gctctccgtc atgttgcgga catagatgcc gaacttcgcc
    13741 tgcgtgcctt cgttcttctc ctcaatgggc ggattgtcgt cgcggaatcg cgggtcgttg
    13801 gcgaacacct tggaccggtc ggcgatcacc agttgccgat ccatgcgctt tccggcgcga
    13861 tccaccgtga tggtcacttc cgtccccacc ggtgtttccg ctacgcggct caccaggtca
    13921 tcaccgtctt tgatggcctt tccgttcatg gcgacgatga tgtcttcggg cttcacccct
    13981 gccttctccg ccgggccgcc ttcctgcacc gactccacca ggacaccctg atcgacaccc
    14041 agcgccttca gcacctcggg cttgtcgttc ttgttccagc ccacaccaat ggagccgcgc
    14101 gtcaccctgc ccgtcttgat gatctggttg tacacgcgca ccgccgtgtt tacgggcagc
    14161 gcgaacccaa tgccctgata tccaccgctc tgcgtggcga tcgccgtatt gataccgatg
    14221 acctcgccgc gaatattcag cagcggtccg ccactgttgc cggggttgat ggcggcgtcg
    14281 gtttgaataa aacgctggaa ttgctgtgca ccggcgatgt cccggccggt cgcactgacg
    14341 atgcccgcgg ttaccgtcgc ctccaaccca aacggcgagc cgatcgcaac cgcccagtca
    14401 cccacctgta cgccgtcgga atttccgatc cgcacggcat ccaactgtct tgccggatcg
    14461 attttgatga cggcgagatc cgtttcgcga tcatagccga ccatcttcgc tttgtactcg
    14521 gttttgtcgt gggccatctt gaccttgatg ccatcggcgc gttcaatcac gtgatagttg
    14581 gtcatgatgt agccgttctt atccacgatg aagccggatc cggttgcttc ctgctgccat
    14641 ggccgctgcg gcatctcggg cgatggcccc tggaagaagc gccggaaaag atccatgcca
    14701 tcttcatctt cctcaccgat cccgggacgg cgattgcggg atgtctggcg ttctctgcgc
    14761 gtgtaatccg ttgtgatatt taccacgctc ggttcggttt gcttcgccag tttggtgaac
    14821 tcgttggaca gctcttgcac gctgggaatg gagagcggcg tggcgtccgg agctgcgacc
    14881 tggtctttag cggcgtttac gccagtggtg atcagagttc cgatcagaat accggcactt
    14941 agggtgaata gaagcagcgc cagcgaaagc agcttctgtt ggcgcatctt actcagaaaa
    15001 ctcatggact ttgttgcctc cgcgagcgat gtggactttc ctcattatag gactgcaccg
    15061 cccgcacttc cggtcgaaat ctggtttccc caggcctccg ggtctaacca gatggatgtg
    15121 gcggaaccgc tgacggtttc aattctacta gcaatatccc tgccagaatc agcacggccc
    15181 cggctacaat cggcaccgta atcgtctcac ggccaagaaa ataggaggtc atcgctgcaa
    15241 ataccggctc cagagcaaag atcagcgcta ctctcgttgc cgtggtaaac cgctgcgccc
    15301 atgcctgcac ggtaaacgcg agcgccgtcg ccagcaaccc cgtcaccgcc acggctccca
    15361 ggacgcccgc ggtccactgg acggtgggaa cttcagccca ccagaaggtc agcgcggacg
    15421 cgccggccgc cgtcgagatt tgcagcgcgc tgagcgcctc gaagttgaca gaactcgaga
    15481 agtatccgat agcgacaatg tgcgcgccaa acgcgaaagc gcatatcagc gtcagcagat
    15541 ctccaacacc gggtataagc gccgccggct caatcgtcat cagggcaagt ccgatggtgg
    15601 ctaccgcgac gcccgcgact tcgaaaactc cgggggcttt cctatagacg aagccgctca
    15661 gcaaaggtac cagcacgatc gacaagccgg taatgaaagc ggatttggac ggcgttgtat
    15721 aacgcagtcc ctgcgtctga aaaaattgac cggtaaacaa taggagtcct gcgataattc
    15781 ccgcccgcag gggcagcttg ccgaagcggt acaagtgctt ccggtacagg atcgccagaa
    15841 cagcggctgc cagcgagaaa cggagcgtta gaaacagaag cgtcgataca tggtcgaggg
    15901 catcctttac gacaacaaac gtcccacccc agataccggt aatgcacacg agggcaagct
    15961 cggcccgcca gtgcgaaacg cgctgccgca tcaggcgatt tccggaagat ccttaagcag
    16021 catcagtagc agcagcagaa tgcgccggag accgagatcg cgcccttcgg ggtgatacca
    16081 ctctgaaggc gtatgcgctc cgcctccctg cccgccggct ccgatcgaca ccgccggaat
    16141 gcccatcgac agcggtatgt tggcatccgt cgaagcgcag tccaggtgcg accggatgga
    16201 aagatgcgca tccaccgccc gtgtgtagcg aaggatttgc gcggcctcgt ccattctccc
    16261 gccgggtctc gatccaatct cgcggatctt cgccgacacg cgcggcccgc tggaccgctc
    16321 attctccgtt tcgagggaac gttccaccgc cgccgtaaga aggctcgcca gctcttcgat
    16381 cttttcgggc gattccgacc ggatatccac cttcgcgcgc gcgacggtcg gtatcgagtt
    16441 gatgctcaac cctccatcga tagtgccgaa attgatcgtg gaccggggtc ccggcgttcc
    16501 tccattcagc cggtattccg tcagcatcga gatcgcacgg cataatgcgt gcaccggatt
    16561 gccggtcccg tagtcgctcc agctatgccc gccggggccg ttgatggcga cctcaaagcg
    16621 ccggctggcg agcgcctggt tggtgatatg gtcgacggcg ggcccgtcca gcacgacaaa
    16681 ccccttgatc ttcggcgcca gggaagattg ccggcacagg taccgcatcc cgctgaggtt
    16741 gccttcgccc tcttccccaa cgttcgccac aatcaggagc gaaccctgca ggtctcccac
    16801 cgccgcgcag gaccgtagcg cagccgtcac ggccagcagc ccggcaagtc ccgctccgtt
    16861 gtccgacacc ccgggcccca aaaacttgcc gtccggcgac acacggatgt cttcggcggt
    16921 tcgcggcgaa agcacggtat ccaggtgagc cgtgagcgcg atatacggac cttccttgcg
    16981 gccgaaggca tgggcgatga cgtttcccgc gcggtctata cggctatcgc atccgagtgc
    17041 gcgaaactgg gcggtcatcc actccgcgcg ctgttcttcc aggaatgtgg gagcggggat
    17101 gcggcagagt tccaggtggc gctcgttgat ccactgcttt tcgcgagtaa accattgcgt
    17161 gcattcgcgt acaaagcgct ccccggccag ttcgcttacg gatttggtat cgataaaagc
    17221 ggtctccgcc actgatgcca gtgtatccta cccccggcgc cggcccccga atccgccggg
    17281 gtaatcgggg gagaccattt gagtttccgc cacgcgatag cataaagttg tctttctcac
    17341 aggagaactt atggcaaatt taaccaggcg gcacgccctt gcgggcgcgg ctaatttcat
    17401 catccttaag ccggaaactg tgttcggttc tcaagccaat tcggccgttt ctttcggcgt
    17461 catcggcacc ggcggcagag gcgtgtatgt gggaacgcac atggcaaacg actcccgcac
    17521 ccggctcacg gccatttgcg atgtctttcc ggatcgtatc gatacggcca agacgcgcat
    17581 gcccggcggc gacaaagcca aagttttcag gaattacaag gacctgctcg gcgacgccgg
    17641 catcgacgcc gtcctgatcg ccacacccgt attcctgcac cctgaacact tcgaagcagc
    17701 cgtcaacgcg cgcaagcata tctactgcga gaagcctgcc ggcgccgacg tagcgggcgt
    17761 gaaacgactg atccgcgcgg gtgcccgggc ggataagacc aaaaccattc agttcggcta
    17821 ccagcaacaa ttcagccccg aatacctgcg tgcgatggag atcctgaaaa caggcaaaat
    17881 cggacaactg accatggtga acagcttctg ggttctcggc ggcactcctc cgaccggttt
    17941 caaatcccct tatcctccgg aagaacagaa gctccggcat tggggatggt ggagggatta
    18001 ctcgggcggg ccgatcgtcg agcaggattg ccatggtgtc gacacgatga actggtacgc
    18061 aggcgctcat ccaacgaaag ccgtggggcg gggtggcctt cgctatccgc tcgcttacgg
    18121 cgactggacc tccgatcatc acaacgtcgt ctactactat ccgaacaaga tcgagggctg
    18181 gctcctgagc atcaagcata cggccgggtt ccgcgatgtg aaggaacagc tctatggctc
    18241 gcagggcatg ctcgaaaccg cacgcaccta tttccgatgg cacgggccaa cgcagaccgc
    18301 accgcttaag aacgcggatg acctcgccga ccggagcctg atcgagaaag cggagtcgaa
    18361 gcgcgagatc acgatcgatg ccgtcgaagc gttcttcacc agcatcgtca atcgcaagcc
    18421 ctacaatatg gcacagagtt cgggagagag caccctcacc gcgctgatgg gccggatggc
    18481 gtttgaaacc ggccgtgagg tcacctggga cgagatgatg aagtcagcgt gatgcttttc
    18541 ccccataacc actccctcac ggtcgtggct cggaaacgaa tactgagccg cgagcgtgag
    18601 cgagcggatg cacaatcatt tctgacgctc cgtttaaacc ggagatcctt cctcgccgcg
    18661 gcgggtggcg cgctgctcgg tcccgcgtct ctggccgccg cccgcgagct tacctgcgat
    18721 gtcgcggtca tcggcggtgg aatggggggc tgtgccgcgg ccctggccgc cctgcgcaat
    18781 ggcatgcgtg tcgtcatgac ggaggagact gattgggttg gcggccagtt gacctcgcaa
    18841 gccgtgcctc ccgacgaaca tgcctggatc gagatgttcg gcgcgaccgc cctttaccgg
    18901 gcgtatcgca atggcgtccg ggaatactac cgcaagcact atccgctaac gcccgaggcc
    18961 cgcgcgcgcg ttcatttgaa cccgggcgat ggcagcgtgt cgagaattac gcatgagccg
    19021 cccgtatcgc tggctgtgat tgaggccatg ctggcgccgc atatcagcgg cgggcggttg
    19081 atcgtgttgc tgtcgcacaa accggtggcg gctgacgtcg acggcgattc ggtgcgggcc
    19141 gtcacggtcc gcagcaccgg gacggcggcg gagaagacca ttcgcgcacg ctatttcgtc
    19201 gacgctacgg aacagggcga ccttctaccg ctcagcaaaa ccgaatatgt caccggtttt
    19261 gaatcgcgca agcagaccgg cgagccgcat gctccggaac aggcacagcc cgcaaacatc
    19321 caggccttta ccgtctgtta tgccgtcgat tacctggaag gggaagatca caccatcgac
    19381 aaaccggaag agtactcctt ttggcgcgat tacgttccca agctgactcc tccgtggccg
    19441 ggcaagcttc tgggctggca catgagcaat ccggttactc tcaaagtgcg caacgtcagc
    19501 ttcgatccct ccgcggacca gaataccggc aacgcactga acctttggat ttaccggcgg
    19561 atcgccaacc gggcgaattt cgtccccggc gcgtaccgtg gcagcatctc cctcatcaac
    19621 tggccgcaga acgactattg gctgggaaat ctccacgaag tcagcgacga ggaagcggcc
    19681 caccatctga agcgcggcaa gcaattgagc ctttcgctcc tgtactggat gcagacggaa
    19741 gcgccgcggc ccgatggcaa acaaggctgg aagggactgc gcctgcgcca cgacatcacc
    19801 ggtacggagg acgggctcgc aaaataccct tacattcgcg agtcgcggcg cattcaggcg
    19861 gagttcaccg tggtggagca gcacgtcggc accgaagctc gtatgcaggc caccggaaaa
    19921 aatcgagagg aactgacagc ggaggagttt cccgattcag tcggcgtcgg cagctatcgt
    19981 atcgatcttc atcccagctc cgggggtgac aactacatcg acgtcagctc gctgcccttc
    20041 cagattccgc tcggcgcgct gattccgaag cgcgtggaaa acctcctggc ggcatcaaag
    20101 aacctggggg tgacgcacat cacgaatgga tgctaccggc tgcatccggt ggagtggaat
    20161 atcggggaag ccgcgggctg tgccgccgcg caagcggttc gccggaacgt accgccgcgc
    20221 aagattcgca gcgaccggcg gctgctcgaa gaatttcaat cggcgatccg cgcacagggg
    20281 atcgaaaccg cctggccgcg tatttccccc aggtagacgc cctatggacg cggcggggac
    20341 cattcgaaca ccggaaccgc cgccgcgcgt cgccgatgtc gaacgcatcg cccgaagcac
    20401 cgatccggtt ctccgcaacc tcgaaatcac cgaatgctat taccaactgg cgtgcgcctg
    20461 ctatgcatgg accggccagc gtgccaactg gtgtacgttc gccacctggg cttcgcggca
    20521 ggctggacgg acgatccggg gcgaagactt cctcgcgaat ctcgaacgtc acctgaacct
    20581 gcggcaatgg ttcacccatc cgatcaaaac gatctggcgg aaaatagtgc gggcgggcat
    20641 ctacaatccg gaaacaacgg tgggacggct ggccagggag attcacagtc cgttcgacgc
    20701 gttcgaacat gccagcgcgg ccgtcgcgcg tggcaacctg aaagtattcg aggagatcgg
    20761 ccgcgaattc gcgcgctttc ttgcggtctg cggcgcggac gcggcgccgg acccggcccg
    20821 catcgcggca ttttgcgagg gtctccgtcc gggcgacccg ccggacggac agcgctacct
    20881 gcgccaggca ttcgccagat attatcagca gcagttcgaa ccggacccct ccgtgcgcgc
    20941 gcaaatgatg tacctggcga acctggagat cggctttcat gagcagacgc gcctgcaacc
    21001 cgagattcgc gaagcgctgg acgtgccggt gcgcacagct catgacatcg gcaagcagtg
    21061 gctgtcgatt ttgtttccgg gcgcgggggc atggaagcga acgctcgaaa aacccgccgc
    21121 agccgtactt ggtgtgatcg cccgtgcgat ctcgcggttg tcggcggacg tggcacggcg
    21181 tgtcatcacc gacgcgatga tgacgctcac cgtccccggc cgcgtcatcg cgctgggccg
    21241 gaacctcgat gtcccggccg atccatcgct cgcccaggtc accctccagg acctggagac
    21301 gatgctttcc aacattgaac ctggacccgg cggcgccgac gattgtggca cggacgactg
    21361 gtcgaacctg cgcgagcgca tgcatttcat cgcgcacctg ttccgcgctt ttgcggctca
    21421 agaatccttg tttcgcgcgc cgttcacatc cgagcaggtc actcaatttc agtcaggcct
    21481 tctgccggat ggagatctgt gcgcttagcg ttgctcaccg cgcgttccgc accgtctcaa
    21541 tcaacggaca ggggcgccct tcgctcgtcg ccaatagggc gcgcccgtcc aggcgatagc
    21601 aaaccgcctc tccctgtttt cgcgggccta gcgacaaggc cgtaaaaggc tggcgccaga
    21661 ttgcatcgaa tccggcgttc gccggcagca ccgcttcgta cgcgcgcaaa tagtcgcaga
    21721 tcaccacgcg gcgtccgtcg ggggaaatat ccgcccctgt cgcgcggccg atcaacagca
    21781 ccagttcgga ttcggcgggc aggttcacat cgccgagcaa ttcgaaggtg gtaacgattc
    21841 cggttcgatg cggagcgcga gcgctgtaca cgctggtgcg tgtttcctcc ccactcgcct
    21901 tcacaatgat gtagatgtct cctgtgtgcg gatgcacggc cagcgcttcg gcgtcgtgcg
    21961 gtgcgtcggg gtagcgaaaa cgcagcgccg tgctcatctg cgtttcggac gtggcgccgg
    22021 gccgtccggc ggcgggctcc tccacgcgga ataccaccac ttcgcggcgg ctgcgccggt
    22081 tatcgccgat atcgccgatg tagaggtagg agcgcccgcg cacaggtcca ggcccgatgg
    22141 cgatgtcttc ccaatccatc gccgtggcgc ccctgacgcg ccatgttccc aagccgcggc
    22201 ccttgcggtc aaacaggtaa agcaaggggg cgccgcccga atcgttatga gtccagaaca
    22261 ggcccggatt ccggcgcgac gccacgatgc cgctactctc gtttacgtcc ctgttttcga
    22321 ggtgtgcaat gacggtgggg gtgccgtaga ccggggtgcg cgcgccgtct ccggtctccg
    22381 aaattcccgc ggtaaagaga aggcaggcca gaattgcgcg cgaatgcaca ttcacgatga
    22441 tagcggccgg ccttactgcc gtttcagcgt cgggcgctca tccctgtcat cgggcttctg
    22501 ctcgccgccg gcatcgattg tgcccgaact tcgccgccga agcgtgggac ggtttggatc
    22561 tttctcacgg actctccggc ggtcgagggc ttcaacccgc gctttcactt cttcgtactc
    22621 cgatgtggtg accacgtact cctcccgtgg gtcgagtccc gtgctcatgt atttttgcgc
    22681 atctttgatg cggtcatccg tcatgggatg gctggagaat acctttgcca gcgttccagg
    22741 tttctttttt tccaacgtct gaagcttttc aaagaaatcc acgaatgccg tgggatcgta
    22801 gccggtcttg tacaggtatt gaatcccata caggtccgcc tcttcttcga agccacgcga
    22861 gaacttcaga aagccaagag gaaccgcaac gccagcggcc tggttcgcga tgtagccggc
    22921 ccatccgccc atgaagatca gcggaatcgt ggcgatattg ataattctgc cgcgcgtggc
    22981 ttgcctggtt ccgtgacggg cggcgacgtg agcgatctcg tgagcgagga cgccggccag
    23041 ttgcgcttcg ttctcggtac gctcgatcag tccggtgttc acgaacaggt aaccgccggg
    23101 caaggcgaat gcgttgactt cgtccgaatc cagcaccttg atggtgaacg gaacttttgc
    23161 atccgagttg cgaaccaggt tctgtccgac gcggttcacg tactcggaga tgacgggatc
    23221 gtccaccaca cgggactgcc gctccacctc ctccgccagt tgctttccaa gcgccatctc
    23281 cttctcgatc gaatagaaat tcacgccttt ggcaatgtca cgctccccga tcatgtccgg
    23341 atctttcttg gcgtcgctgg cccaggcagc cggcacgaat gcattgaggg tgaataagac
    23401 tacgaccaga agggccttga ctctttgcat tttgaaaatt cctctggtgc gggcaaaggt
    23461 gtctgcgcaa cacccagtgt cgctaacttg ttggacgcga tgcaaagccg gtttggtttc
    23521 ttccggttgc gctagcattg tggttcgagg ttttcgggtt atgcgatcgc tcctgctctg
    23581 cttctttttt gtcctggttg gctgcggtgt ggcaacgtct ttcgaaatca agtgggtcca
    23641 tctttcgagc agaaacggcg atttgcccga ccctggtggc tcgaaccagc agaccgctct
    23701 cctcgccgcc gatttcgata agagcggcgc aacggcgttc atcgtgggct gccgggttaa
    23761 ggcgcccgcg ctggttctat accggcgttc cggaaactcg tggacccgct cagttatcga
    23821 aaacgaatat ctcccgatcg aggcgggcgg agccgccgcg gacatcgatg gcgacggcga
    23881 cctggatgct gtgttcggag gcgattacca aagcaaccag ctctggtggt gggagaatcc
    23941 ccatccccga ttcaacccgg agacatcctg gaaacgccac gtcataaaga acagcggtgc
    24001 gaaccagcac cacgatcaga ttttcgcgga cgtgaagggc tccgggaaac cccagctctt
    24061 attctggaac cagcgcgcca aggcgatcct tcaggccggt attcccacgg acccgcgtaa
    24121 cgcggcagcc tggcccctgg aaacgctgta ctccggcggg gcgggagaca acgtggagaa
    24181 tgcagcgaag tacgccgaag gcgtcgatgc cgccgacgtg gatggagacg ggcgcgtgga
    24241 cctgttggcc ggcaactcct ggttctaccg ggatgaagta ggccgctacg ttccggttcg
    24301 aatcggctcg atcggcggcc gcatcaagac gggcaggttc cagcgcggaa agcttccgca
    24361 agtggtcatc gccccgggtg atggtagtgg accgctgcgg ttctactggt cctcgggcaa
    24421 tccgcgcagc gccgaatcct ggtccgggcg cactctgctg gagcgcgaca tggtgcacgg
    24481 acatacactc gacgtcggcg atatcgacgg cgacggccat ctcgatattt tcgctgcgga
    24541 gatggcgcaa tggaccagga acccacaacc cgaccacccc gaggcgaaag cctggatcct
    24601 ctatggcgat ggcaaaggaa actttcgcaa caccgttctg gtcaccggac acggatggca
    24661 cgaaggcaaa ctggccgacg tagatggaga cggagacctg gacatcatca acaagcctta
    24721 cacctggaac accccccgga tcgacgtttg gctgaacaat ggaacggccc cggcacgacg
    24781 caagtaatgc gaagggtcag gagcgtggtg gtaacggccg cattcgaatt ctctgacgcg
    24841 tgataacgct gaagtatcca gcccaagatt tctgagatcg aaccgccgag actgcgagtg
    24901 aagcggcctc gtctgggctt tgcggcatga gccggaagag cataatcccg cactgcgccg
    24961 gcaactttcg ccgatacgcg agttcgccga agtctttgtc aaatgttagt agcgtcctgt
    25021 gcgtggaagc ggctaaggca acacttcatc gtcggaagcg ccaggattcg cctccgcgat
    25081 ccacacgaca tcaacgccgg ctcttcgaag cgcttccact gccggacgag ggaagttctc
    25141 gtcggcgagg aaggtcatgc gggttccagg gggaagacct tttcgctatg caggatctcg
    25201 ctcgcatacg cgagacaggc acgaacatcg ttagcgttga gcgtcgggta gctgtcgaga
    25261 acctgctgtt cggtccaacc cgcggccagc agatcgatga ccagctcgac agagatccgc
    25321 gtgcctttaa caatcggttt cccgacgagg atctgcgggt ccacgccgat atggtcgcgc
    25381 cagtccatgc cttcaccgta tcagaacagc cggattcagg cgtataatgc cggaaccact
    25441 ttgcccttcc cgtctttggt gacgtaaaac ggacgtttct ccaccacata cgacgtgtcc
    25501 ggggcgatgc ccatggcgtt gtaaatcgta gcgtgcaggt cctggattgt gattggattc
    25561 tcgacgatgc cgcaagggcg ctcgtccgca gtccggccgt agagaaaccc tttcttgaag
    25621 ccaccgccga atagcagcac actgctcgcc tccgtaaagt ggcggtgcat tccgtaatgc
    25681 ttcaggtcgg tcagaacgtc gggctgctgc acctgttcct tcaccgtctt gccaggcttt
    25741 ccttccgtca tcatgtcgcg gccgaattca cttgccagca cgatgatcgt acggtccagc
    25801 aaacctcgct cttcgaggtc gagcacaagc tgcgaaacag gtccgtcaat ctgttgcttc
    25861 attccggcgg cgcgtgtgtg cccgttctcg tgcgtatccc agtaccggaa gggaatgtac
    25921 tctgtcgtta cttcgataaa acgcgtgccc gcctccgtga gtctgcgtgc cagcaggcac
    25981 cccagcccga accggccggt gttgtagacc gcgaacgatt tttccggctc gagcgacagg
    26041 tcaaacgctt tcgccgcggg agaagtcaga agccggtgcg cgttctcgat ggaccgtgtc
    26101 agcgattccc gctggtaatc gctgccgtac ttcatcaccg gactattctg cgccagcttc
    26161 ttgtagttct cataccgccg ccggaatctc tcctgcgtca tgtgcccagg cgggcgcaca
    26221 gcggacgccg catcgcgggg gtccgctatc agaaacggac catactcgga accgagaaac
    26281 cctgcggtat gaaacgcctt cacagcgtcg ctttcaccac cgatctccag gttctgtccg
    26341 atgtcgatga acgcgggaac atccggattc cgggacccaa gcgtcttcgc gacgaaagct
    26401 cccatgtgcg gaacagcgag cgattgcggc ggctcgtatc cggtgtgcca gtgaaactga
    26461 tgccgcgaat gaaggatgaa tcccagatcg cccacacgat gcgaccgtat cagcgtggcg
    26521 cggtccatca ccttcgcgat ccgctccagc ccttgagaaa tttgtatgtt gtcgaccgcc
    26581 gtgcggatag aaggaaaagt gctcaagacg cgccgcgctt ccattccgct ttcaaacggc
    26641 gtgtacgtct tgggatcgaa ggtttcggtc tgcgccattc cgccggccat ccagagcagg
    26701 atcaaggtgt cagcgcgcgc cgccggtttc ggcgaagagg ctccgtggag caggtgcggt
    26761 tcgccacctt gcaaagcggc taacgctcca ccggctgcgg cgcgcataaa atcgcggcgc
    26821 ccgaggccac ggttcaattc gtccaggatc ctccgcatct cattcctcct ttcaccggat
    26881 gaactggaac tccggcagca tcactacggc ccacaggaag tcttccagtc cgtcgctgtt
    26941 gagttttacg ctcctaccgc cgccaaaaaa ctgtcttgcc gatgcccgtt ctctcgtgga
    27001 aggctcacgt ccaagtgcat gcaggtagac gcgcgagatg gcgtcgtcga cgctcgtcag
    27061 agcaggcggc gggggaacgg gtggagcgcc ggccacgcgc accaactgca tgcggtccgg
    27121 ctcctcagtg aaaacaaaag tcctgacgct gggagagatg tcgcttgtct gggaagactc
    27181 atccacgccc actgtcgctt gaaaacgggt aaatcgcctg ccggaaagat cgaagaccaa
    27241 ctcggaggac aacggtgcga gcaccgcggg ttgggcatcg tgccctttta ttttgagcgt
    27301 tcccgtttgg aaggccggac gggatgggag ttccgccagc gggacctccc cttccgggcc
    27361 ggttaatcgc gcgccggtcc atccggcggt tacgcgcgcc gggtcgtaag agtcgtgatc
    27421 ttcgagaagc aaacgcagct cccgcgctcc ggagatctcg acatcgacat ggatcgcatc
    27481 ggaccggaag acaccgctat cgaaacggtt cggcggcggc gtgggcaact ggcccagcat
    27541 tcgcagcgct ccacgatgga gcagcgttgc agccgtctcg ccattcgcca tctccagcgc
    27601 ttgcagagta gaaggagccg tattgcgctc ggtcaccgcg gaatcgcgaa tggggcgccc
    27661 cagggtgcga gtaaggctgc tcgctttcag ccgccactcc cggctgtagg tgccctcgcc
    27721 cgcttttcgc ggctgcaata tgcgccattc accggtcatc gcggagatgg catctacaaa
    27781 ttgctccgcc gtcaaacggc ggtactgcgg gccgcggaag acgaacttct cttttcggtc
    27841 ggctggatcg gcggctgaat ccacagcggg cagttgatat gtcctcgaat tcaggatggc
    27901 ccgcagcagg tattggatat cgtagccgtg ttcggcaaag ctggatgcga gccagtcgag
    27961 caggtccgca ttccagggct cggcatccag gtcgtccacc ggctccacca ggccgcggcc
    28021 gaatagctgc ttccagacgc ggttcacaaa ggttcgcgtg aaccggccgt tttcgggtga
    28081 cgtgaacagg cgcgctgcaa cctccctttt ctctttcagg ggtgcatcgg tagcgacccc
    28141 gccgagctcg ggatagagga accgcgccac cgccgtcttg cccgtcggta catcgcagcg
    28201 caccagctcc agcggcttct ccgagtagaa gctcgcgagc ccgtaggcgt ccgcaagctt
    28261 ccatttgctg ataaagctgt cgtggcagga attacatttc agattgacgg cgagaaagac
    28321 ctgcgcgctg ttttgcgccg cctgcatcac cgccgtctgg ctggcgttca catcgccgcg
    28381 ccaattgacg cccagcagga atccttccgg atcgctgtct tcggtaggat tgagcagcgc
    28441 ccgggcaaac cggtcataag ggaggttctt ctgaagagcg tccaccagcc agggcgtaat
    28501 cgacttgcgg tcgccgacat agctcacgcc ttcgtcattg tgcagcaggt cgttccaaaa
    28561 actgatccag tgcccgctgt acggctcctc cgccgcgagc agtttttcga tgaggcgctc
    28621 gcggctgtcc gggccggtgt tgttcaggaa ttcgaggcgc tgctgcggtg tgggcagcag
    28681 tccccagaga tcgagataca cccgccgcag gaacacttcg ccggagacgg cctccggaaa
    28741 agccacaccg gccttctgaa aatatgcggc catgaaccgg tcgatggggt ttcggaagct
    28801 ggtgttctcc ggaacatccg gacggcgcgg ggaaagcact ggggtccagg atggctcttc
    28861 gatagcacca ggaccgggag cgccggcgtc gatccagcgg cgaatgacgt cgatctccgc
    28921 cgccgagagc ggcttgccgg ccagcggcat caagggctgt ttttcgccgg tgattcgcgc
    28981 taccagaagg ctgttcgcgc tggaaccggc gacgatggcg ggaccgctca cgccaccttt
    29041 gagcatttcc gcgcgcgtgt ggaggcgcag accggcttgc gcttcgcttc cggtgtggca
    29101 ggccgcacag cgcgcattga agatcggctg cacatcgcgc gcgaagctgg catcagcggc
    29161 cgatgattga gcgcgaacct ggcacatcgc gagcacaaga aacaacactg gaactccggc
    29221 agacagccgg aggcagaacc tgcccctcat gtgtccacga tacacgctcg ccgcgcggga
    29281 agccacatcc gtggacgcaa ctggccggtt gccgctgtta gaataccttg ctaggacaaa
    29341 catgaccagc ttgactcgtc gttatacgcg atgtgtgcta ccggcgctcg ccgccggcct
    29401 gttcgtggca ctggcgggat gctcccggcc ggcggaggag ccgaaaaaag aggaacccgc
    29461 cgagcggcct gccgccgaag cgcctcccgc ggcggaagca cctaaggaag ttccaactcc
    29521 gcccgcggag gcaaaacccg aggctaaaaa ggcggagacc aaacccggcg tggtgcccgc
    29581 cacctacaaa gtacggctcg ataccagcaa aggtcctgtt ctgatcgaag tgcagaaggc
    29641 atgggctccg ttgggcgccg agcagttcca caagctcgtt acatcgggct attacgatgg
    29701 agcgcgcttt ttccggatcg tgccgaattt catcgtgcaa ttcggcctgg cggcagatcc
    29761 gaaggtgacc aaaaagtggg ataggaacat caaagacgat ccggtgacga gaaccaaccg
    29821 tctcggtgca gtgacgtttg ccaccgcagg cccgaatacc cggacgacgc aagtgtttat
    29881 caacctgaag agcaaccagt ttttggacga tcagggattc gcgcctttcg gccaggtcgt
    29941 ggaaggcatg gatatcgtgc agcaactcta tagcggctac ggcgaagcgc cggatcagga
    30001 tgccatcacg aaccgcggca acgcctatct gagcgcgcag tttccgaagc tcgactacat
    30061 caagaaagcc actatcgtcg aatagcaacg gtagagagta tcgatgcgcc taccacgccc
    30121 aggcgacgcg gtacccgtat ggatcggcgc ccgtttccag cacaccattg ctcagtattc
    30181 gtatcatcga cggcgcggcc ccactggacc accgcgtgcg gcgcgctatc ttatggccgc
    30241 gtgacgccag ttcctcgaaa acaccctgct gaatacgctc gtctagcatc aactcgccgg
    30301 gattcatcgc gtgattatcg aagctggata ccaggtgcct ggtctgaaag cgcggcgctt
    30361 cgacggctgc ctccgcgttc attccgaact cgaccacatt caacaggagt tgcagaagag
    30421 cctgatcctg cgcgtcgccg ccgggcgtcg aaagcgcgag atagggtctg ccgttgcgcg
    30481 tcacgagcgt tggactcaac gtgacacgcg ggcgcttgcc cggagcgagt tcattggggt
    30541 gccccgggat caacagaaag ctctgcgccc tcgatgtgag cggaattccg gtgtccccgg
    30601 cgatcaccga tggcagccag ccaccggacg gcgtagccga aaacacgagg ccgtcgcggt
    30661 ccgctgtatt aatgcacgtc gtatcccttg ccatcaacgc gtcgtcgatg cgaacccggg
    30721 gaatttccga ttcggaagga tgcagggcgc ggatgcctgg aaacgccccg ggccggaatt
    30781 cgagcgatgc tttgggagag atcgcatcac gacgctcagc cgcatactct ttcgacagca
    30841 gccttccgga tggaaccttg acgaacgccg gatcgccgta atacgtgtca cggtcggcgt
    30901 aggccagttt cagcgcttcc gtcaagacat ggatataatc ggccgagttg tgccccatgg
    30961 cgcgcaggtc cagaccctcg agaatattca aggcctcgat cagcgaaggt ccctggctcc
    31021 agaaacccgg cttgtagacg gtgacaccgc ggtagtccgt tgaaaccggc tcctccggtt
    31081 tcagccggaa agcggccatg tcttcgtagc gcagcagacc tccattcgcc cggctgaagg
    31141 cgtcgatttt tcgggcgatg ccaccgcgat agaaataatc gcggaccgtg tcgatggcgg
    31201 ctttgcggct cgcacccgag gcccgcgcct cttcttcttt cttcaccatg tcgcggagcg
    31261 tccgcgcaag atcgggttgc cggaagatct ctccgggaag ggttacacgg ccctcgggaa
    31321 ggaacacctg cttggaagtg ggccagcggt tgaaaaatcg tctgctgcgg gcgatggttc
    31381 cggcgcgggt ttcatccatg ggcatgccgt ccgcaagctc aatggcaggc tgaatcactt
    31441 cggcgaaagt cctggttcca aattcgccga gtgccaccag agcggcatcc accattccgg
    31501 ggaccagcgc cggcatcaat ccggcaaccg gcaccattcc cttcagccca ccgggttcga
    31561 gatcttccac ttcacccgct tccaagcggc gttggcgaaa gaaatcggcc gaggcgagct
    31621 taggcatcgt gccaaccccg gcgatggaaa agacacgtcc gtctttggag cggatcagga
    31681 tcggagcttc gccgccaaat ccgaagtggg aatattccgc aaccgcggcg gcgaacatgg
    31741 ccgctacgcc ggcatcggcg gcgttgcctc cggcatgaaa caatcgcatt ccggcgtcgg
    31801 cagcgaacct gcttccagcg ccgatggccc cgcgtgtgcc cctcgccgga tgctgtatca
    31861 gttgttcgga actctttcgc tgcgccccca gaaaagatgc gatcaccagt accaatagag
    31921 ggacttggag tttcctcatc gctaaagcca attataatgg gcgatatcca aatgacgtcc
    31981 gtctccacga ggggatatcg atgagtgatg tgaatgagct gctggagcga ttccggcgcg
    32041 gtccggaact gctcgcggtg gccactaccg gcgcggccgg ttcggaactc gatttcgttc
    32101 cgggaccgga aaaatggagt gtgcgccaga tcgtctgcca tcttgccgac gcagaactgg
    32161 aaggagcggt ccgcttccgg cgtgtaatcg ccgaagagaa tccggccttg attgcttacg
    32221 accaggaggc ctgggcgcgc catctcggtt actcgcggcg gaaactatcg caggcgctcg
    32281 aaagctttcg ccggatccgt tcggagaatc atgaactgct gaaagcgttg ccagaggagg
    32341 cattcagccg gacagggaca cacaacgagc ggggtgtgtt gtcgcttctc gacttgttga
    32401 agatctatac ggaacatgcc gaacagcatg cacggcaaat ccgcgaggta cgggatgcat
    32461 acaaaacaca caggacttca aatggctgag gcgccgggaa atgcaagtgt atgacactgc
    32521 cgcgcctgta tcccatcgtg gatcgcgaca cgctgattcg agttcaatgt ccggttcaaa
    32581 ccgccgtcga agtattgctg gatcgcggta tccggttcct gcagtttcgt gtcaaaggcc
    32641 atatcggccg tactgagttc gcggagttcg aatgcgtggc ggaactctgc gcgcgcgcgg
    32701 gggcagcact catcgtgaac gatcgggccg atctcgcgat gctgctccgc gcgggggttc
    32761 atgtcggcca ggacgacctt tcgccaaagg acgtgcggcg tctggtgggt gaaaatcgct
    32821 tacttggatt gtccacccat aacgaagatc agttccgtgc gggcatcgcg gagccggtgg
    32881 actacctcgc tttcgggccg atttttgcca ccaccaccaa agagaatccg gatccggtag
    32941 tcggcttgga gatgctgcga atgttgcgcg gactcactac gaaaccgctg gttgccatcg
    33001 gcggcatcac gcgagcgaat gcagcatccg tgctcgaagc gggcgccgat tcggtggctg
    33061 tcatcggtga cctgtatccc gctgtcttta cgctgggcga atttcgaaag cgagtaaacg
    33121 aatggcaaca actcgtcagg tagataccgg gttcatcaag ggcctcggcc tgctggactc
    33181 cacaacgctg gtcatcggtt cgatgatcgg gtcgggcatt ttcattgtct cggccgatat
    33241 tgcgcgccag gttaattcgc ctgggctgct gattatccca tggctcgtta ccgcggttct
    33301 gacgatgatt gcggcgctca cgtatgggga gttggccgct gcaatgccgc gcgccggcgg
    33361 ccagtatatc tatctgcggg aagcctatgg cccgctgttc ggattccttt atgggtggac
    33421 gttgttcctg gtaatccaaa caggaaccgt tgccgctgtg gccgtcgcat tctcgaagtt
    33481 tgctggtgtg ttctttccct ccatctccgc aaccaattac gtaatacgcg cagggggctt
    33541 tcacgtcaat acacaacagg ccctggcaat cggtgtcatc gtgctgctga cggcggtgaa
    33601 cacccgaggg attcacacag gcgccatggt gcagaacatc ttcacattcg ccaagaccgc
    33661 ggcattgctc ggcttaattg tgctgggctt cctggtgggc cggaacgctg ccgcgatcga
    33721 agtgaatttc accgatttct ggcggggcgc cgattggagt tggaccgtga ttcgcgttgt
    33781 tggagtggcc atggtgggag cgttgttcgc ctccgatgcg tggaacaatg tcaccttcac
    33841 cgc
//
LOCUS       DASTMK010000325        12575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_272067, whole genome shotgun sequence.
ACCESSION   DASTMK010000325 DASTMK010000000
VERSION     DASTMK010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272067"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..875
                     /locus_tag="VFQ79_14130"
     CDS             180..875
                     /locus_tag="VFQ79_14130"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEU0140856.1"
                     /translation="MYSPAMCVLVAGLCAGNALSQARPVNRMEITLERNGVSGWTPVH
                     PGFVLSSNDRVRFRFRSNFPGYLYVMNQGTSGDYALLFPREDTGSQNRIEAGKEYVIP
                     ATEGHFRVTGPAGHDIVYWLVTPADLGGESSSPLAHVPLPAAPKDPEARKSLTPRCDE
                     TLLRARGDCIDGSAGAKGIAASGELPENLRAVPGVSGSRELIFLRSDQSSVVSSPVPL
                     QGPVVYEFRLAHR"
     gene            881..2962
                     /locus_tag="VFQ79_14135"
     CDS             881..2962
                     /locus_tag="VFQ79_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0140857.1"
                     /translation="MRIARLALSGIFVLFLAAGGRGEQQQSRDLKYEKESSKPETAAP
                     QTAKVSIPRSYAVVIGIANYAKLPDSAQLKFSERDAEAIYSILISPEGGNFRAENVKK
                     LIGAKATLANIRKELEQWLPGITKDDDRVLIYFAGHGFVYGGKGYLAPYDFDKSDIAG
                     SGYSMETLGRVIGSSIKGKWKVLLTDSCHSGAITPEADVQTLNRSLLDLNKSLFSLTA
                     SRDRERSFESPEWGGGHGIFTYYVVKGLEGTADESGDGIVTADELANYVRTEVRQATA
                     GKQNPTSERGSFDPDMLLAYVPSGVRPGAPPAPKFGTLIFESNMDGVEVFIDGKSAGV
                     VNKGKPLRLPGLAPGAHTIKGVRMGYEPDGPREEMVYPGQESTVSLKIVIPRRRSKAA
                     LDRFEEGLEFYNKGSIENYKRAAERFRAALAADPDYSQAALYLARALRDSFEFQESRK
                     AFEQAITIDPDYLEARATYGGMLLDVGDEDEAIRQLNTVVTRNPDHALAQYLLAQALA
                     RKELFAQSIEAARKAIALTPNNAEAYFWLGESLRMTGKYGEAVRAYSDYLRLSDFDSK
                     LAGKMNYYVLGFLVGAGRKKRAAQRDVWKELRGLAYFGICDSERKLGNFDAAIENCRT
                     SLSLDSSDPYAHYALGLSYLAKGKNDGAVEMLPAARTQFRKMLDLNPELVEAQYARKN
                     IAAIDDFLAGR"
     gene            2977..4746
                     /locus_tag="VFQ79_14140"
     CDS             2977..4746
                     /locus_tag="VFQ79_14140"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEU0140858.1"
                     /translation="MVLRRLLAVPWMGLALFAQPSVMPLNEIRPGMRGVGKTVFSGDR
                     VDQFDVEVLGVLENVGPKQSLVLARLSGGPLQHTGVLQGMSGSPVYIGGRLVGAVAMS
                     FPYAKDPIAAIRPFEEMTRERFTGRDAQRAGISLWETDLTKNIARSTDVPAGESRLMD
                     IATPVSFAGFTRSTIEHFGAQLKSLGFEPRQGVAGGGKPAARLGNPAKLEPGSMISVQ
                     LLTGDMSVGADGTVTAVEGKHVYAFGHRFLSIGDTQLPFARAEVLTLLPNLSSSFKIS
                     TAREWMGTITADYNTAVAGELGRRASMIPVSIEVQRKSSNGKPISSAGYRMEMVNDRY
                     LSPFLLQMAVFSAIDATERTVGVSSFLLRGHVEFENAAPLKLDNAYAGEFNVPAQVSL
                     GAAMPVAYALQSGFDALTLKNVSITVEAFSQNKQLQVAGAWASREAVRPGESVDLTVL
                     LAGENGMEISRTVSYQVPVGAVPGPLFFTAADGNTTNLTEYRNVIGEQPKSPSRVLSF
                     LNGLRGNNKAYVRVWRASPGYQIEGEDLPNPPASVSMVLGHGPANQAGWAVPRNAKVA
                     EMAIDTGGMIVTGAKTVQVEIKE"
     gene            4743..6989
                     /locus_tag="VFQ79_14145"
     CDS             4743..6989
                     /locus_tag="VFQ79_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140859.1"
                     /translation="MIRPFALSVALGGLLLQGASTTAWEMSTYQDFLRGRFTGVSLTK
                     SGRLMLSPKLETVLASDQPVIWSIVRGKDGSIYAGSGHRGRLYRIDASGKSSVLWTAD
                     QSEIFALALDPSGTLFAATSPDGKIYRIRDGKAEEYFAPGAKYIWSLAFDKDGVLYAG
                     TGDQGKVFRVTAQGRGEVYYETGQLHITSLAFDPEGRLLAGSDPNGILYRISAKDKAF
                     VLYDANLPEIRAVVPSADGSIYAVALGGATVNRTPASLTPSGVTPGGAPAAAPTVSIT
                     VTDEAQGGIEVKPQPSDPKQQTPAAQTQVTTQFTPIVDMTGVEKSAIYKIHPDNTVET
                     LWSSKEENAYDLIVYGTDLVFSTDGQGRIYRLSGDRKPTLVVQTDQGETTRLLDANEG
                     FYAATATLGKVLRLGTGVTASGAYESPVHDANTVARWGRLSWKANREGGAKLAFRTRS
                     GNSARPDQTWSEWSEPLEDASNSLVSSPNARYIQWRAEFSSSGDVSPSLDSVSLAYLP
                     QNTSPVIRSITVASSISASAQKPAAQQPPAAAAYSITVTDTGDTAASTSTGTPTETLS
                     RAGSEQLVVSWQADDADGDKLVYSIFFRGEDEREWKTLRENIPETSLTFDGDVFADGK
                     YYFLVRASDRLVNPLDSARESELVSAPVLIDNTPPLVQISAPRRSGAAVELDIQGADQ
                     ASPLRKAEYSIDAGPWTPLQAQDGIVDSEREQFRLRLETLSPGEHLVVVRVYDSASNA
                     GLAKIVLR"
     gene            complement(6990..8444)
                     /gene="pyk"
                     /locus_tag="VFQ79_14150"
     CDS             complement(6990..8444)
                     /gene="pyk"
                     /locus_tag="VFQ79_14150"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU0140860.1"
                     /translation="MHRFVNQPGASLRRTKIVATLGPASDSDDVIRQLFYAGVDVFRM
                     NASHGTPEEHTRRIHTVRAIARELDVHTATLLDLQGPKIRLGEFEGGGCVLKTGSLFV
                     ITTEPVLGNCDRAATTYANLPNDVKPRDRILLADGSVELRVLSSDGTSVRCEVTSGGQ
                     VSSRKGINLPGVQVSTPSLTSKDMSDLQFGLKAGVDLLALSFVRNPKDILRLRVYLEQ
                     QDVKLPIVAKIEKPEGWENLDGILEESDGVMVARGDLGVEMALEQVPVIQKSIIERAR
                     MRGKFVITATQMLESMIENVLPTRAEASDVANAIFDGTDAVMLSGETSIGRYPVETVR
                     MMSRIARATETAMQHAALKPASHIPKPTHAEIIADAAYAAARSAGVSAIVVFTSSGSS
                     ARLIARYRPPIPIYAFTPSDATARQLAAVYGVRPVIAPHTESTDEMLQQMDRMLVSRG
                     WVEPGGSVVFVAGQPIGRPGSTNMIKLHTIGERR"
     gene            8535..9098
                     /locus_tag="VFQ79_14155"
     CDS             8535..9098
                     /locus_tag="VFQ79_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU0140861.1"
                     /translation="MVCELDGAKPVNSFALVSYIPDPLGGFLDRLRQELVPSCLLHSH
                     VSILPPRPLDGHADEAFGRICSRMKQMNAFELHLGDVEIFENTAVVYIAIREGFEELK
                     AMHSILNAGALSFEEPHSYHPHITLAQDFPREQLHELAARAARCWSEFDQRRAFPIEE
                     AAFVQNTDRNMWINLGHCSLSRVPAAL"
     gene            complement(9106..9588)
                     /locus_tag="VFQ79_14160"
     CDS             complement(9106..9588)
                     /locus_tag="VFQ79_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140862.1"
                     /translation="MAGSTTKKVVVQRFDREPISGFVNPQTYLSAVGVELLSLSGNLI
                     ILSYEEIKTVYFVREFLSGDPGKEKREFATRPKTAGLWVRLRFRDGDKLEGILANDLL
                     QIEPPGFTLVPPDPLSNSQKVFIPRQALSGIHVVGVIGGARRQRRRTEPAREQLKMFE
                     "
     gene            9647..10540
                     /gene="xerD"
                     /locus_tag="VFQ79_14165"
     CDS             9647..10540
                     /gene="xerD"
                     /locus_tag="VFQ79_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU0140863.1"
                     /translation="MPSADELTARIDEYLDFCRMEKGLADNSLDAYSHDLAKFRSFFA
                     NRGGIPDTADVRRYLDHLWEKGLSSRSISRNLTTLRNFYVFLLRENYIASDATALISL
                     PRQWKVLPKFLNEGEVDRLLAAPDPARANGLRDRAMLELLYATGLRVSELCQLELNAI
                     ELDLGLVRVTGKGGKQRIVPAGKHAILAVRAYLESGRTALLRGRGSRYLFVTSRGGCL
                     TRQGFWKLLGRYGRRAGVFRRLTPHVIRHSFATHLLEHGADLRSVQTMLGHADISTTQ
                     IYTHVTRSRLRKTVDEHHPRA"
     gene            10600..11898
                     /locus_tag="VFQ79_14170"
     CDS             10600..11898
                     /locus_tag="VFQ79_14170"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140864.1"
                     /translation="MSDYMFMLESHLTAGEARVLADVQAAAAEANVNLFLAGGAMRDM
                     LGGFVIRDLDFTIEGNAVKLARKLAQKTGAKILAVDDHRKCVELLFPGNVTAEIGMAR
                     QERFPKPGGRAQVSPATIYEDLKGRDFTVNSIALSLNKSSRGLLLDPNNGLADLEHKE
                     LRAVTNYTLYDEPVRVLRLIRFKVRLGFTIAEKTEAQYRAVREAGLESKIGPRSLLHE
                     LTQIAAEANAVEVVRALDQEGLLRLFSPALTGQKVNLAGLARLQKARQFVPFGVDFQI
                     ENTGLFLNILTEKLTPKEKSAMAGALKMEKPEIDAWQKLDARVKKLETALKSPRLQKP
                     SLVYKALENVPGEEILFLYLRTGVRVVQDRIRNYLQRYLPTAQEVTEADVAAPAGVET
                     GSPKYAKFHQAAITARLDARPKKVSPPPEPETQATAGARK"
     gene            11941..>12575
                     /locus_tag="VFQ79_14175"
     CDS             11941..>12575
                     /locus_tag="VFQ79_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140865.1"
                     /translation="MNRRAFLVGAGAGLSVLGANQRINVGVVGVRGRGRDHIAEFVKL
                     PNARVAAVCDIDDAQIERAIAFTQKQQQGLHPRSYRDLRKLLEDKDIDAISIATCNHW
                     HALATIWACQAGKDVFVEKPASHNIFEGRKMVEAARKYKRIVQAGMQSRSIEHKKQAI
                     QLLREGAIGKVYMAKGLCYKRRKSIGKQPDGPVPAGVDYDLWLGPAPMRPF"
BASE COUNT         2577 a         3704 c         3830 g         2464 t
ORIGIN      
        1 acgctctgat cgggaagatc cgccaggaga tggtggctcg cgacctggta tttgaaaaag
       61 tggatgagag cacgcccggc gcgaagaaag aaagcgcgat ctacgtagtg aatcccagcg
      121 gcgacccgga cgcacgcctg atcgtcgatc tccagttgaa gcatcggtga aggagcgtga
      181 tgtacagtcc cgccatgtgt gtcctcgttg ccggcctatg cgccgggaac gccctctctc
      241 aagccagacc cgtgaaccgg atggagatca cgctggagcg caacggggtc tcaggatgga
      301 ctcctgtgca tcccgggttc gtactgtctt ccaacgaccg tgtccgcttc cggtttcgtt
      361 ccaattttcc gggctacttg tacgtgatga accagggcac atcaggcgat tacgcccttc
      421 tgtttccgcg tgaagatacg ggctcgcaaa atcgtatcga agcggggaag gaatatgtga
      481 ttccagccac ggaagggcac tttcgcgtta cgggtcccgc ggggcacgat atcgtatact
      541 ggctggtcac gccggcggat ttgggtggag agagttcgag cccgctcgcc catgttcccc
      601 ttccggcggc gccgaaagac ccggaagccc gcaagagcct gacgccgcgc tgcgatgaga
      661 cgctgctgcg cgcacggggc gattgcatcg acggcagcgc gggggcaaag ggtatcgccg
      721 cctccggcga gttgccggag aatcttcggg cggttcccgg cgtgagcggt tcccgggagc
      781 tgatcttcct gcgcagcgac cagagctccg tcgtatcgtc gccggtgcct ttgcaaggcc
      841 cggtggtata cgaattccgg ctggcgcaca ggtgagagcc atgaggattg cgaggctggc
      901 actgtcaggc atcttcgttc tgttcctagc cgcgggcggg cggggcgaac aacagcaatc
      961 ccgggacctc aagtatgaaa aggagagctc gaaaccggag acggccgctc cgcaaacggc
     1021 caaggtttcc attccacgca gctacgcggt ggtgatcggt atcgccaatt acgcgaagct
     1081 gcccgactcc gctcaattga agttttctga gcgcgacgcc gaagctattt attccattct
     1141 tatcagcccg gagggtggca atttcagggc ggagaacgtc aagaagctga tcggcgccaa
     1201 ggcgacgctc gccaacatcc ggaaggaact ggagcagtgg cttcccggca tcacgaaaga
     1261 cgatgaccgg gttctgatct attttgccgg gcacggtttt gtttacggcg gaaaagggta
     1321 tctggcccct tatgacttcg acaagtccga tatcgccggc agcggttatt cgatggaaac
     1381 gcttggaagg gtcatcggaa gttccatcaa aggaaaatgg aaagtcctgc ttacggattc
     1441 ctgccacagc ggagcgatca ctccggaagc agatgtgcaa acgctcaacc gcagcctgct
     1501 ggacctgaac aagtcgctgt tctctttgac cgcaagccgc gaccgcgagc gttctttcga
     1561 gagcccggaa tggggaggcg gacacggcat cttcacctac tacgtagtga agggcctgga
     1621 gggaaccgcc gacgagagcg gcgacggcat tgtcactgcc gacgaactgg cgaattacgt
     1681 tcgtaccgag gtgcggcagg cgaccgcagg gaaacagaac cccacttcgg agcgaggcag
     1741 cttcgacccg gacatgctgc tggcctacgt gccttccgga gttcgtcccg gggcgccacc
     1801 tgcgccgaaa ttcgggaccc tgatcttcga aagcaacatg gatggcgtgg aggtgttcat
     1861 cgacggcaag tccgccggtg tggtgaacaa gggcaagccc ttgcggctcc cgggactcgc
     1921 gccgggtgca catacgatca agggagtgcg gatggggtat gagccggatg gcccgcgaga
     1981 agagatggtt tatccgggcc aggagagcac cgtcagcctg aagatcgtaa tcccgcgccg
     2041 gcgtagtaaa gccgcgctcg accggttcga agagggactg gagttctaca acaagggctc
     2101 tatcgagaac tataaacgtg ccgcggagcg tttccgcgcc gcgctggccg ccgaccccga
     2161 ctatagccag gcagccctgt acctggcacg cgctttgcgg gattcgttcg aattccagga
     2221 gtccaggaaa gccttcgagc aagctatcac gattgacccc gattatctcg aagcgcgcgc
     2281 aacctacgga ggaatgttgt tggatgtagg cgacgaggac gaagccatcc ggcagcttaa
     2341 taccgtggtg acacgcaatc cggaccacgc gctggcgcag taccttctgg cgcaggcgct
     2401 ggcccgaaag gaattgttcg cgcaatccat tgaagccgcg cggaaggcca tcgcgttgac
     2461 gccgaacaat gccgaagctt atttctggct tggcgagagc ctccgcatga ccggcaaata
     2521 cggcgaggct gttcgcgcgt actccgacta cctgcgtctc agcgatttcg acagcaagct
     2581 ggcgggcaag atgaactact atgtgctcgg gttcctggtt ggcgccggga ggaagaagcg
     2641 ggcggcgcag cgcgatgtat ggaaggaact gcgggggctc gcgtacttcg gaatctgcga
     2701 cagcgaaagg aagttgggaa actttgacgc tgcgatcgag aattgccgca catccctgtc
     2761 gcttgactca tcggatccct acgcccacta tgcgcttggg ctgagctatc tcgcgaaagg
     2821 gaaaaatgac ggcgcagttg aaatgcttcc cgccgcgcgc acgcagttcc gcaagatgct
     2881 ggacctgaat ccggaacttg tggaagccca atatgcccgg aaaaacatcg ccgccattga
     2941 tgacttcctt gccgggcgtt aattacactt agagtagtgg tactccgccg tctgctcgcc
     3001 gtgccctgga tgggtctcgc tctatttgcg caaccctccg taatgccgct taacgaaatc
     3061 cggcccggca tgcgaggggt tggtaagacg gttttttccg gcgaccgcgt cgaccagttt
     3121 gatgtcgagg ttctgggagt gctcgagaac gtaggcccca aacaatcgct ggttcttgcc
     3181 cgtttatccg gaggcccgct ccagcatacc ggcgttcttc aggggatgag cggaagccct
     3241 gtctatatag gcggccggct tgttggtgcc gtggccatgt cctttcccta cgcgaaagat
     3301 cccatcgccg ccattcggcc tttcgaggag atgacgcgag aacggttcac ggggcgcgat
     3361 gcgcaacgtg ccggcatttc gctttgggaa accgacctga ctaagaacat tgcccgcagt
     3421 acggacgttc cagccggcga gtcgcggctg atggatatcg ccactcccgt ttcctttgcg
     3481 gggttcacgc gcagcaccat cgaacacttc ggcgcacagt tgaagtcgct cggctttgaa
     3541 ccgcggcagg gcgttgcggg cggaggcaag ccggccgccc gcctcggcaa tccggccaaa
     3601 cttgagcccg gttccatgat cagcgtccaa ctcctgactg gggatatgag cgtcggcgcg
     3661 gacggcaccg tcaccgctgt agagggtaag cacgtatacg ccttcgggca ccgcttcctt
     3721 tccatcggcg atacgcaact cccgttcgca cgcgcggagg ttctcacgct tctgccgaac
     3781 ctgtcgagtt ccttcaagat ctcaaccgcg cgggaatgga tgggcacgat tactgcggat
     3841 tacaacacgg cagtggcggg tgaactcggc cgccgcgcca gtatgattcc ggtgtcgatc
     3901 gaagtccagc ggaagagcag taatgggaag cccatcagtt ccgccggcta tcgcatggag
     3961 atggtgaacg accgttacct ctcgccgttc ctgctgcaaa tggcggtttt ttccgcaatt
     4021 gatgctaccg aacgcaccgt cggcgtttcc agctttcttt tgcgcgggca tgtggagttt
     4081 gaaaatgccg cgccgctgaa gcttgacaat gcatacgccg gcgagttcaa cgtgcccgcc
     4141 caggtttcgc ttggcgccgc gatgcctgtc gcttatgcgc tgcaaagcgg tttcgatgcg
     4201 ctgacgttga agaatgtgtc catcacggtg gaagcgttca gccagaacaa acaactccag
     4261 gtggccggcg catgggcttc gcgcgaagcc gtccggccgg gagaatccgt ggatttaacc
     4321 gtgttgttgg cgggtgaaaa cggaatggag atcagccgca cggtgtcata ccaggtgcct
     4381 gtcggggcag ttcccggtcc gctttttttc acggcggcgg acgggaatac gaccaatctc
     4441 accgaataca ggaacgtgat cggcgagcag cccaagtctc cctcccgggt tctttctttt
     4501 ctgaacggtc tcaggggaaa caataaagct tacgtgcgcg tatggcgggc aagtcccgga
     4561 tatcagatcg aaggagagga tctgccgaat cctcccgctt ccgtttccat ggtgctcgga
     4621 cacggtccgg caaaccaggc cggctgggcg gttccgcgga acgccaaggt ggcggaaatg
     4681 gcaatcgata ccggtggcat gatcgttaca ggtgctaaga ctgtacaggt ggaaatcaag
     4741 gaatgatccg gccgttcgcc ctgtccgttg ccctgggcgg tctactgctt cagggcgcca
     4801 gcactacagc gtgggaaatg agcacgtatc aggatttcct gcgtggccgg tttaccgggg
     4861 tttctctcac gaaaagcggc cgtctgatgc tctctcccaa gctggagacg gtactcgcat
     4921 ccgaccagcc ggtgatctgg agcatcgtcc gcgggaaaga tggctccatt tacgccggca
     4981 gcggacatcg cggacggctc tatcgaattg acgcgtccgg gaaaagttcc gtcctctgga
     5041 cggcagatca gtccgagatc ttcgccctgg cactggaccc atcgggaacg cttttcgccg
     5101 caacctcccc cgacgggaag atctaccgga tccgcgatgg aaaagcggaa gaatacttcg
     5161 caccgggcgc caagtatatc tggtcgcttg ccttcgataa agatggcgtg ctctatgccg
     5221 gcaccggaga ccaggggaag gtgtttcgcg tgacggcgca ggggcgcggc gaagtttact
     5281 acgagacggg gcagcttcac atcacgagtc tcgccttcga cccggagggg cgcctgctgg
     5341 cgggaagcga cccgaacggg attctctacc ggatttccgc aaaggacaag gccttcgttt
     5401 tatacgatgc gaatcttccg gaaattcgcg ccgtcgtgcc gtccgcggat ggaagcatct
     5461 atgctgttgc cctcggcggc gcgaccgtca accggactcc cgcatcactc actccctctg
     5521 gtgtgacgcc gggcggggca cctgccgccg ctccaacggt cagcatcacc gtcaccgacg
     5581 aagcacaggg aggaatcgag gtgaagccgc agccctccga tcccaagcag cagaccccgg
     5641 ccgcgcagac acaggtcacc acgcagttca ctccgatagt ggatatgacc ggagtggaga
     5701 aatccgccat ctacaagatc catcccgata acacggtgga gacgctgtgg agctcgaagg
     5761 aagaaaatgc ttacgacctg atcgtatatg ggacggacct ggttttctcc accgacgggc
     5821 aaggacggat ttatcgcctg agcggcgatc gaaagcccac cctggtggta caaacagatc
     5881 agggcgagac aacaagactg ctggatgcca acgagggctt ctatgcggca acggcgacgc
     5941 ttggtaaagt gcttcggctg ggcaccggag taacggcgtc gggcgcttac gaatcccccg
     6001 tgcatgacgc caacaccgtg gcgagatggg ggcggttgag ctggaaggcg aacagagagg
     6061 gcggcgcaaa actcgcgttc cgcacacgtt ccggcaactc cgcccggccc gatcagacat
     6121 ggagtgaatg gtcggagccg ttggaggacg catccaacag cctggtgagc agtcccaacg
     6181 cgcgatacat ccaatggcgc gcggagtttt cgagctccgg ggatgtctcg ccctccctgg
     6241 acagcgtttc gctggcgtat ctgccgcaga acacctcgcc cgtgatacga agtattaccg
     6301 tcgccagcag catctccgcg tccgcgcaaa aaccggcggc gcagcagccc ccggccgcgg
     6361 ctgcatatag catcacggtt accgatacgg gcgatacggc cgcttccacg tcgacgggaa
     6421 ctccgaccga aaccttgagc cgggccggaa gcgagcagct tgtcgtctcc tggcaggccg
     6481 acgatgccga tggcgacaag ctggtgtact ccattttttt ccgcggcgaa gacgagcgcg
     6541 aatggaagac cttgcgggag aacatccccg aaaccagcct cacattcgat ggtgacgtgt
     6601 tcgccgacgg caagtactat ttcctggtgc gcgcctccga caggctggtt aacccgctgg
     6661 attcggcgcg cgagtccgaa ctggtgagcg ctccggtgct catcgacaat acgccgcccc
     6721 tggttcaaat cagtgcgccg cgccgttcgg gtgcggcagt ggagttggac atccagggcg
     6781 ccgatcaggc ctctcccctg cgaaaggcgg aatactcgat tgacgccggt ccgtggacgc
     6841 cgcttcaagc gcaggatggc atcgtcgact ccgagcgcga gcagttccgg ttgcggctgg
     6901 aaaccctgtc gccgggcgag cacctggtgg tcgtgcgagt ctacgattcc gcatcgaacg
     6961 ccggccttgc gaaaatcgtg ctccgttgat cagcgccgtt cgccgatcgt gtgaagcttg
     7021 atcatattcg ttgagccggg ccggccgatg ggttgccccg ccacaaacac cacgctcccg
     7081 cccggttcca cccatccgcg gcttaccagc atccggtcca tctgttgcag catttcatcg
     7141 gtcgattccg tgtgcggcgc gatcaccgga cggacgccat aaactgcggc cagttgccgg
     7201 gctgtggcat cgctcggagt aaacgcatag atgggaatcg gaggccggta gcgtgcgatc
     7261 aggcgggcgc ttgacccgct gctggtgaac acgacaatgg ccgagacgcc ggcggagcgg
     7321 gcggcggcat aggccgcgtc ggcgatgatt tccgcgtgag tgggcttcgg gatgtgcgat
     7381 gccggtttga gcgcggcgtg ctgcatggcg gtttccgtcg ctctcgcgat gcgcgacatc
     7441 atacgcacgg tctccaccgg ataccggccg atggaggtct cacccgacag catcaccgcg
     7501 tcggtaccat cgaaaatcgc attggcaacg tcgctcgcct cggcgcgagt cggcagcaca
     7561 ttttcgatca tcgactccaa catctgggtg gcggtgatta cgaattttcc ccgcatccgc
     7621 gcgcgctcga ttattgattt ctgaatcacc ggcacctgct ccaatgccat ctccactccg
     7681 aggtcgccac gcgccaccat cacgccgtcc gattcttcca gaatgccgtc gaggttttcc
     7741 cacccttccg gcttctcaat cttcgcgaca atgggcaatt tcacatcctg ctgctcgaga
     7801 tacacccgca gccggaggat gtccttcgga ttccggacga acgataacgc gagcagatcc
     7861 actcccgcct tcagcccgaa ctgaagatcg gacatgtctt tactggtcag ggatggcgta
     7921 ctcacctgaa caccgggcag attgattcct ttgcggctgc tcacctggcc gccgcttgtc
     7981 acttcgcatc gaaccgacgt gccgtcgctc gacagtacgc gtaattccac actgccgtcg
     8041 gcaagcagaa tccggtctcg cggcttcaca tcattcggaa ggttggcgta ggtcgtcgcg
     8101 gcgcgatcac aatttccgag caccggctcg gtagtgatga cgaagagcga tccggtcttg
     8161 agaacgcaac cgccgccctc gaactcacca agacggatct tcgggccttg gaggtccagc
     8221 agagtggccg tgtgtacatc caattcccgg gcaatggcac ggacggtgtg aatgcgccgg
     8281 gtgtgctcct ccggagtccc gtgcgaggca ttcattcgaa acacatccac acctgcgtaa
     8341 aagagttggc ggatgacgtc gtccgaatcc gatgccgggc ctagtgtagc gacgatcttt
     8401 gtgcggcgca gagaagcgcc cggctggtta acgaaccggt gcatagatgt atgatctctt
     8461 gttcaggttc attccgcatt ggacgcttcc gggataagca tacttcggac ttttcgctta
     8521 cactggtttg agaaatggtt tgtgagctag acggggccaa accagttaat tcgttcgcac
     8581 tggtttccta tatccccgat ccgctgggtg ggtttcttga tcgcctccgt caggaactgg
     8641 tgccgtcgtg tctgttgcat tcccacgtca gcattctgcc cccgcgacct ctggacgggc
     8701 acgcggacga ggcatttgga cgcatctgct cgcgaatgaa gcagatgaac gcttttgagc
     8761 tgcatctggg cgatgtcgag atcttcgaga acacggcagt tgtgtacatc gcgatccggg
     8821 aagggtttga agaactgaag gccatgcata gcattctcaa cgcaggcgct ctttcgtttg
     8881 aagaacccca cagctaccat ccgcacatca ccctggccca ggattttccg agggagcaat
     8941 tgcacgagtt ggccgcccgc gccgcccgct gctggagcga gttcgatcaa aggcgggcct
     9001 tcccgataga ggaagccgcg tttgtgcaga acacggatcg caatatgtgg ataaacctgg
     9061 ggcactgctc gttgtcgcgg gtaccggcag ctctctaagc tcccgttatt cgaacatctt
     9121 caactgctcg cgggcgggct cagtccttct tcgctggcgt cgcgctccgc cgattacacc
     9181 gaccacgtgg atgccggaaa gcgcctgacg gggaatgaac accttctggc tgtttgagag
     9241 cggatcggga ggtaccaatg tgaacccggg cggctcgatt tgaagcaggt cgttggctag
     9301 gatgccttcc agcttatccc cgtcccggaa gcgcaggcgt acccacaacc cggcggtctt
     9361 cggccgggtt gcgaactcac gcttttcttt tcccggatcc ccgctcaaga actcgcgtac
     9421 gaaatagacc gtcttgatct cttcgtaaga gaggattatc aggttccccg acaagctcaa
     9481 taactccacg ccgactgctg aaagataggt ctgcggattc acaaaaccgg atatcggctc
     9541 ccggtcgaat cgctgcacga ctactttctt tgtggtagac cccgccaaaa tacttccatc
     9601 ctcagttttc tttaaacatt ttcgcgagta ttgtatcatt ggcacgatgc ctagcgcgga
     9661 tgagctaacc gcccggatcg atgagtatct ggacttctgc cggatggaaa agggcttagc
     9721 cgacaatagt ttggacgcct acagccacga cctggctaaa ttccggtcgt tttttgcgaa
     9781 ccggggtgga attccggata ccgcggacgt tcgccgctat ctcgatcatc tctgggagaa
     9841 agggctgtcc agccgctcga tttcccgaaa tctcacgacc ctgcggaatt tttacgtatt
     9901 cctgctgcgc gaaaactaca tcgcatccga tgccaccgct cttatttcct tgccccggca
     9961 gtggaaagta ctgccgaaat tcttaaatga aggggaggtt gacaggctcc tggccgcccc
    10021 cgaccccgcg cgcgcgaatg gcttgcggga ccgggccatg ctggagctgc tgtacgccac
    10081 cgggctgcgt gtatccgaat tgtgccagtt ggaattgaat gcgattgagt tggatctggg
    10141 gttggtgcgg gtcaccggca agggcggaaa gcagcgtatc gttcctgccg gtaagcatgc
    10201 catcctggcc gtaagagcct atctcgaaag cggccggacg gcgcttctgc gagggcgggg
    10261 aagccgctac ctgttcgtga cctcgcgggg cggctgtctc acccggcaag gtttttggaa
    10321 gttgctcggc cggtacggaa ggcgagccgg cgtgttccgg cggctgacgc cccacgtcat
    10381 ccggcacagc tttgccacgc acttgctgga acacggcgcc gatctccgca gtgtccagac
    10441 catgctcggg catgccgaca tctcgaccac ccagatctat acgcatgtca cccggtcgcg
    10501 gttgaggaaa acggtagatg aacatcatcc gcgggcctag gagcttgttg aaaaacgtag
    10561 aaaaccgctt gctgacgcgc gcggctcgga gctactgaca tgagcgacta catgttcatg
    10621 ctggagagcc acctgaccgc cggggaggcg cgggtgttgg ccgacgtgca ggcagcggcc
    10681 gccgaggcca acgtcaacct gttcctggcg ggcggcgcca tgcgcgacat gttgggagga
    10741 ttcgtaattc gcgacctcga cttcaccatc gagggcaacg cagtcaaact ggccaggaag
    10801 ctcgctcaga agaccggcgc gaaaatactt gccgtggacg atcaccgcaa gtgtgtggaa
    10861 cttctatttc cgggtaacgt gaccgcggag atcgggatgg cgaggcagga gcgtttcccc
    10921 aaacccggcg gcagggctca ggtctcccct gccacgattt acgaagatct gaaggggcgg
    10981 gatttcaccg ttaattccat cgcactctct cttaataagt cctcccgcgg gctgctgctc
    11041 gaccccaaca acggtctggc agacctggaa cacaaagagc tgcgcgcggt aacgaactac
    11101 acgctctacg acgaaccggt acgggtgctc cgccttatcc gcttcaaagt gcggctcggc
    11161 ttcaccattg ccgaaaaaac tgaggctcag taccgggccg tgcgggaagc cggcctggaa
    11221 agtaaaatcg ggccgcgatc gctactccac gaactgacgc agatcgcagc cgaagccaac
    11281 gccgttgaag tggtgcgcgc cttggaccag gaaggcttgc tgcgcctgtt ctcaccggcc
    11341 cttaccgggc agaaggtcaa cctggccgga ttggcgcgcc ttcagaaagc gcggcagttc
    11401 gtaccgtttg gcgtcgattt tcaaatagag aatacagggc tgttcctcaa tattctgacc
    11461 gaaaagctga ctcccaaaga gaagtcggcc atggccggag cgttgaagat ggaaaagccg
    11521 gagattgacg cctggcaaaa gctggatgca cgggtaaaga aactcgagac agctttgaag
    11581 tctccgcggc tccagaagcc ctcgctggtg tacaaggcgt tggagaacgt ccccggcgag
    11641 gaaattcttt tcctgtattt gagaaccgga gtccgtgtcg ttcaggatcg gatccggaat
    11701 tacctgcaaa gatatctccc gacggcgcag gaggtaacgg aagccgatgt ggccgcgccg
    11761 gcaggtgtgg aaacggggtc gcccaaatat gccaaattcc atcaggctgc catcaccgcg
    11821 cggcttgatg cgcggcccaa gaaagtttcg ccaccaccgg agccggaaac ccaagcgacc
    11881 gccggcgcgc gtaaatgaca tccgcgccaa cgtgcacatc cccgctgtag aattgcactc
    11941 atgaaccgcc gagcgtttct cgtcggcgcc ggtgcgggtt tatccgtgct tggtgcaaac
    12001 cagcgaatca acgtaggtgt cgtgggcgtc cgcggacgcg gacgcgatca cattgccgaa
    12061 ttcgtaaagt tgccgaacgc gagggtggcc gcggtgtgcg atatcgacga cgcacaaatc
    12121 gaacgtgcca tcgccttcac ccaaaagcag cagcaaggct tgcatccacg ctcttaccgg
    12181 gacctgcgca agctgctgga agacaaagat atcgatgcca tctccattgc cacctgcaat
    12241 cactggcatg cgctggcgac gatctgggcg tgtcaggcgg gcaaagacgt tttcgtggag
    12301 aaacctgcca gccacaacat tttcgaaggc cggaagatgg tagaggcggc gcgcaaatac
    12361 aaacgcatcg tacaagccgg catgcagagc cgcagcatcg agcataagaa acaggcgatt
    12421 cagcttttgc gcgaaggggc gattggcaag gtctatatgg ccaaggggct gtgctacaaa
    12481 cggcggaaat cgattgggaa acagccggac ggccccgtcc cggcgggagt cgattacgat
    12541 ctgtggctcg gtcccgctcc catgcggccg tttaa
//
LOCUS       DASTMK010000326         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_623934, whole genome shotgun sequence.
ACCESSION   DASTMK010000326 DASTMK010000000
VERSION     DASTMK010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623934"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFQ79_14180"
     CDS             complement(<1..463)
                     /locus_tag="VFQ79_14180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0140866.1"
                     /translation="MPGRRTRRASAAVFALLLSHVSFAQDYRAKVQGVVTDSSQAVVG
                     GAKVTLTNLNTGISATKETNAVGQYIFEFVEPGTYSLSAEQPGFSKFVQQSFAVQVRA
                     DITVDPVLQVGNLADQVTVTETMTGVKFNTSSVDLTIDRKMLTDLPILGRNP"
     gene            complement(609..1706)
                     /locus_tag="VFQ79_14185"
     CDS             complement(609..1706)
                     /locus_tag="VFQ79_14185"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0140867.1"
                     /translation="MLTTRRDALSTALFVAAQSARASQSARSKTGMPGPFPGRVIAVH
                     SPGCILSDQYQKEPVRQMIERGVMELTGAPSAVEAWRMFVRPGDVVGIKLNPNGMPRV
                     ISAPEVVHPVIDGLKAAGVKARDIVVFDRNRAFIVKAGYDKWLPEGIRYAWASETYDA
                     IQLDMDGYDRDCYAELPVIQPGQDPNDRHFRRSYVARFVTKEINKLINIPVLKHHQSA
                     GVTLALKNIAYGMANNVARSHQNSTLHCTSVFIPAIMDLQPFREKTVLHIVDGVKGVY
                     HGGPAARPGYIWEHKTIYFATDPVAVDKIGWKALDRKRAEMSLAPIALSKPDKVSTRL
                     NCQPEHIEIAGALGLGEFDDAKIDLRQIELA"
     gene            1819..2331
                     /locus_tag="VFQ79_14190"
     CDS             1819..2331
                     /locus_tag="VFQ79_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140868.1"
                     /translation="MRTWALPLLFTVCLFAESAAGVRWTVPAGWKSEGPRPMRAASYA
                     VTATQPDTENGECVVYFFGAGQGGGIEANIERWKSQFQSAGKPPAAKVNKKTVNTLPV
                     TTIDMSGTYTGMGGPMVAQKTAKPGYRLLGAIIEAPGGNLFVKFTGPAKTVAANQQKF
                     EQLVASFKKN"
     gene            complement(2341..>2828)
                     /locus_tag="VFQ79_14195"
     CDS             complement(2341..>2828)
                     /locus_tag="VFQ79_14195"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU0140869.1"
                     /translation="GEGSPRQPLLLVDAGTPKEVARSRMAAQRDGAAPTGHGFPLPNE
                     TGEAPGNIVIAGGDVPVDQMVASLDRGILVTRLWYIREVDPYEKIMTGMTRDGTFLVE
                     GGRIRCGVRNFRFNQSLIEMLRNVQALSPSARVSGEETFDMVVPAMQVADFQFTEVTR
                     F"
BASE COUNT          580 a          823 c          814 g          611 t
ORIGIN      
        1 atggattgcg gcccaggatg ggcagatccg tgagcatctt gcggtcgatc gtcaagtcca
       61 cactcgaagt gttaaacttc actccggtca tggtttccgt aactgtgacc tggtcggcca
      121 ggttccctac ttgcagcacc ggatctacag tgatatccgc gcgtacttga acggcgaagc
      181 tttgttggac gaatttgctg aagccaggct gctcggcgct caacgaatac gtgcccggct
      241 cgacaaattc aaatatgtac tgcccaacgg cgttggtttc tttcgtagct gagatgccgg
      301 tgttgaggtt ggtcagggtc actttcgccc cgccgacaac ggcctggctg ctgtcagtta
      361 cgacaccctg tactttcgca cggtagtcct gcgcgaagga aacatgggat aggagtaatg
      421 cgaacactgc ggcggatgct cgccgcgtcc ttctgcccgg catagtatga cctcctgaag
      481 gaacaaccca tctttttcga aaaaagaggt cattcccgtt actgattgga ggtattctaa
      541 tgcagatcgg gtcaggattc cacgaaaact ttgttgagtt ttctaaattt gcccgagtta
      601 acggctattc aagccagttc aatttgccgc aagtcgattt tggcgtcgtc aaactcgccg
      661 agtccgagcg cgccggcgat ctcgatgtgc tcgggctgac aattcaggcg cgtgctgacc
      721 ttgtcgggct tggatagcgc tatcggcgca agactcatct ccgcgcgctt ccggtccaga
      781 gccttccagc cgatcttatc cactgcgacg gggtctgtcg cgaaatagat cgtcttgtgt
      841 tcccagatgt agcctgggcg ggcggcgggt ccgccatgat atacgccttt caccccgtcg
      901 acaatgtgga gcaccgtctt ttcacggaat ggttggagat ccatgatggc gggaatgaac
      961 acggacgtgc aatgcaatgt gctgttctga tgactgcggg cgacattatt cgccatgcca
     1021 tacgcgatat ttttcagcgc cagggtaaca ccagccgatt gatggtgctt caacaccggt
     1081 atgttgatga gcttgttgat ttccttcgtt acgaatcggg ccacgtacga acggcggaaa
     1141 tgccggtcgt tgggatcctg ccccggctga ataacgggca gctccgcgta acaatcacgg
     1201 tcgtagccat ccatgtcgag ttgaatggcg tcgtaagttt ccgaagccca ggcatagcgg
     1261 atgccttccg gcagccactt gtcgtagccg gcctttacga taaacgcgcg attccggtcg
     1321 aagaccacga tatcccgggc tttgacaccg gctgccttca gcccgtcgat gaccgggtga
     1381 acgacctccg gagcggaaat gactctcggc attccgttgg ggttcaactt gattcccaca
     1441 acatcacccg gccggacgaa catgcgccac gcctccacgg cggacggagc accggtgagt
     1501 tccatcacgc cgcgttcgat catctgccgg acaggctctt tctgatactg gtccgagagg
     1561 atgcaccccg gcgagtggac cgcgatgacc ctgcccggga atggtcccgg cattccggtt
     1621 ttcgagcgcg cggattggga cgcgcgagcg gattgggcgg cgacgaataa agcagtggaa
     1681 agagcatccc tgcgtgtggt gagcatcggc gagcctcctt cgccggagga ttcaaggact
     1741 tttacctccg gatagccact acgattctat cccgcccggg acggacgcgc actcggcctc
     1801 agaggctaaa ctggtgacat gcgtacctgg gcacttccgc tattgtttac tgtgtgtctg
     1861 tttgccgaat ccgccgctgg cgtgcgctgg acggtgccgg ccggttggaa gagtgagggt
     1921 ccgaggccga tgcgtgcggc aagttacgcc gttaccgcca cgcaaccgga taccgaaaac
     1981 ggcgaatgcg tcgtttactt tttcggcgcc ggacagggtg gcggcatcga agcgaacatc
     2041 gagcgctgga aaagccagtt tcaatccgct ggaaaaccgc cggccgcaaa ggtaaataag
     2101 aagacggtca atactcttcc cgtaaccacc attgacatgt ccggaaccta taccggcatg
     2161 ggcgggccca tggtggcgca aaaaacagcc aaaccgggat accgcctgtt aggtgcaatt
     2221 atcgaggctc ccggcggcaa tctgttcgtc aaattcaccg gtccggctaa gactgtggcc
     2281 gccaaccagc agaagttcga gcagcttgtg gcatcgttca agaagaactg aggcaatgcc
     2341 tcagaagcgt gtcacctcgg tgaactgaaa atccgccacc tgcatcgcgg gcacgaccat
     2401 atcgaatgtc tcttcgccgc tcacccgtgc ggaaggcgaa agcgcctgca cgtttcgcaa
     2461 catttcgatc aaactttgat tgaatcgaaa gtttcgaact ccgcaacgga tgcggccgcc
     2521 ttccaccagg aaagtgccgt cgcgcgtcat gccggtcatg attttctcgt acgggtccac
     2581 ttcccggata taccagaggc gtgtcaccag aatgccccgg tcgagtgaag ccaccatctg
     2641 gtctaccgga acgtctcctc cggcgatcac gatattcccg ggagcttcgc ccgtctcgtt
     2701 cggcagagga aagccgtggc ccgtcggcgc cgctccgtcc cgctgtgccg ccattcgcga
     2761 gcgcgcgact tccttgggcg ttccagcgtc caccagcaga agcggctgcc ggggagaccc
     2821 ttcgccat
//
LOCUS       DASTMK010000327         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_17547, whole genome shotgun sequence.
ACCESSION   DASTMK010000327 DASTMK010000000
VERSION     DASTMK010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17547"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFQ79_14200"
     CDS             complement(<1..527)
                     /locus_tag="VFQ79_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU0140870.1"
                     /translation="MPAGTKFELKTGVATREAFGKTLVELARENPDIVVCDADLSKST
                     MTHWFAAEFPDRFFSCGIAEADMVGIGAGLASSGKIAFVSSFSCFVLNKGFEQLRVTA
                     AYPNVNLKVVGTHSGISIGEDGPSQMSIEDTSLACSLPGFVVLSPADEVTMRALVRAA
                     VQHVGPVFIRAGRPKV"
     gene            complement(527..1348)
                     /locus_tag="VFQ79_14205"
     CDS             complement(527..1348)
                     /locus_tag="VFQ79_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU0140871.1"
                     /translation="MTHSKSLDELQAIAKRVRRQIVEMITAAKSGHPGGSLSAVEILV
                     TLYFDVMRHDPKNPQWPDRDRFLLSKGHAAPVLYAVMAEAGYVPADQLNTLRKLGSIY
                     QGHPDRRFIPALDASTGSLGQGLSLGLGMAAAARLDKSPTRVYVVLGDGEIQEGQIWE
                     AAMFGAFHKVDNIVAIVDYNRIQLDGFVKDIMELEPLAAKWESFGWHVIEIDGHNIPA
                     LQKAFDEAANVKGKPVALIAHTVKGKGVSFMENNPKFHGQAPTAAELEAALKELA"
     gene            1448..1861
                     /locus_tag="VFQ79_14210"
     CDS             1448..1861
                     /locus_tag="VFQ79_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU0140872.1"
                     /translation="MAEDVRQVEYYSVLIPNKIGEGSRVLGALRDEGVNFTGIWAYPQ
                     GKRQARMDLVPEDAAAMKKALRKIGVAVESTQKGFLVSGDDRPGAVADALAKLAGAGI
                     NVRAAQAVCAGQGRFGSFIELEPEDVRKAKKALGA"
     gene            1973..2557
                     /locus_tag="VFQ79_14215"
     CDS             1973..2557
                     /locus_tag="VFQ79_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140873.1"
                     /translation="MRLLIPARAAVLAATLCSAQASGGPEAAPLKLHQGENGTYRLSL
                     DTLKRAFQAYRNVLETVAQQPGLLERIRAEAACLPESEAAGRDKLSLLMSRMERTEPA
                     LAAAFRKAGTSPKEAGMAMEALAGSMIIYGIIENTTENERYSSNKNVAFIKNNKKTIS
                     AMLSEFAASGKRFSALIEGTQEPDEEPGGEGPGK"
BASE COUNT          580 a          789 c          795 g          514 t
ORIGIN      
        1 actttcggcc gtcccgcgcg gataaacacc ggacccacat gctggacagc ggcacgaacc
       61 agggcgcgca tcgtcacttc atccgccggc gagagcacga cgaaccccgg gagcgagcag
      121 gcgaggctcg tgtcttcaat actcatttgc gacggtccgt cttcgccgat cgaaataccg
      181 ctgtgcgtcc ccaccacttt caggttcaca ttcggataag cggcggtcac ccgcagttgc
      241 tcgaatccct tgttcagcac gaaacaggaa aagctcgaga cgaaagcaat cttcccgctg
      301 gaggccaggc cggcgccgat gcccaccata tcggcctcgg cgataccgca cgaaaagaac
      361 cggtccggga actctgctgc gaaccagtgc gtcatcgtgg acttggaaag atccgcgtca
      421 caaacgacga tgtccgggtt ctcccgggcc aattccacaa gagtttttcc gaacgcctca
      481 cgcgtggcca ccccggtttt cagttcgaat ttcgttccgg ccggcattaa gcaagctcct
      541 tcaacgcagc ttccagctcc gcggccgtgg gcgcctggcc gtggaatttg ggattgttct
      601 ccatgaacga cacgcccttg cccttcaccg tgtgggcaat caacgccacg ggtttgccct
      661 tcacattggc ggcttcgtcg aatgctttct gaagcgccgg aatgttgtgg ccgtcaatct
      721 ctatgacatg ccagccgaaa ctctcccact tggcggcgag aggctcgagt tccataatgt
      781 ctttgacgaa accgtccagt tgaatgcgat tgtaatcgac aatggctacg atattgtcaa
      841 ccttgtgaaa cgcgccgaac atggccgctt cccagatctg accttcctgg atttcgccat
      901 cacccagaac cacgtacacg cgggtggggc ttttgtccaa tcgcgccgcc gcggccattc
      961 ccaacccgag ggaaagcccc tggcccagcg aacccgtcga ggcatcgagt gcggggataa
     1021 agcgtctatc cggatggccc tggtagatcg agcccagctt ccgcaaagtg ttcaattggt
     1081 ccgccggcac gtagccggcc tcggccatga cggcatagag caccggcgcg gcgtgtcctt
     1141 tggaaagcag aaaccggtcg cggtccggcc actgggggtt cttcgggtcg tgccgcatca
     1201 cgtcgaaata taaagttacg aggatttcaa ccgccgaaag ggacccgccc ggatgacctg
     1261 attttgccgc cgtgatcatt tcgacgatct gccgccggac cctttttgca atggcctgca
     1321 attcatccag gctctttgag tgcgtcatgg gtgtaagatt tcaatgtacc atgtggcctt
     1381 gctgccggag tccgatgacc cggaagccaa gtcggtggta tgtccaccaa tagaagggag
     1441 agttgctgtg gctgaagatg ttcgtcaggt tgaatactac tccgtcttga ttccaaacaa
     1501 aatcggagag gggtcgcgtg tccttggcgc actacgcgac gaaggggtga acttcaccgg
     1561 aatttgggct tacccccaag gtaagcggca agccaggatg gacctggtgc cggaagatgc
     1621 ggcggcgatg aagaaagcgt tacgcaaaat cggcgtcgcc gttgaaagca cgcagaaggg
     1681 atttctggtt tcaggcgatg accggccggg agctgtcgcc gatgcactgg ctaagctcgc
     1741 cggtgcgggc atcaatgtac gggctgccca ggcggtctgc gcggggcaag gccgcttcgg
     1801 ttccttcatc gaactggagc ccgaggacgt ccgcaaggca aagaaggctt tgggcgctta
     1861 acgcgaagcg ccggaccagc ctatggtacc ggcgctccat tcgtgaggtt ccgccgtctg
     1921 agccaatccg ggaaagagct gcgcgttagt ttgcaggagt ataggagaca ccttgcgatt
     1981 gctcatccct gcacgcgccg cggttctggc cgccactctc tgttctgccc aggcatccgg
     2041 cggacccgag gctgcccctc ttaagctcca ccagggcgaa aatggaacct accgtttgtc
     2101 cctggatact ttgaagcgcg cgtttcaggc ataccggaac gtgctggaga cggtggcgca
     2161 gcaacccggc ctgctagagc gaattcgggc agaggctgcc tgcctgccgg aatccgaggc
     2221 ggccggcagg gataagctta gtctcctgat gagccgcatg gagcgcacgg agcccgccct
     2281 cgcggccgcc ttccggaagg cgggcacatc gcccaaagaa gccggaatgg cgatggaggc
     2341 gctcgccgga tcgatgatta tttacggaat tatcgaaaat acgacagaaa acgaaagata
     2401 ttcaagcaat aaaaacgtag cgttcataaa aaataataaa aaaacaatca gcgccatgct
     2461 aagcgaattc gcggcaagcg gaaaacgctt ttcggcgctg atcgaaggaa cgcaggagcc
     2521 tgacgaggaa ccgggcggag aagggcccgg caagtagcct accggcgaag ccggcgtata
     2581 gcgccaaaaa caccggcgag tactcgcgcc gggtccgggt cttcagcgcg aatgatttcg
     2641 atgggtccgt tgcccgtcgg acgccagcgc tcgaaggt
//
LOCUS       DASTMK010000328         7685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_496530, whole genome shotgun sequence.
ACCESSION   DASTMK010000328 DASTMK010000000
VERSION     DASTMK010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496530"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFQ79_14220"
     CDS             complement(<1..767)
                     /locus_tag="VFQ79_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU0140874.1"
                     /translation="MQTVAIIGVGLIGGSFALALRKAGFAGTILGVSSRKTLDKALEL
                     EAIDEPSTLSDAAHRADLLYLAQPIRAILETIALLEGCLKPGALITDAGSTKREIVEA
                     AAAIRSAQFLGGHPMAGKEVRGVGAADAALFAGRTYLLTPRQPDEMDTDAVREFRRWL
                     ERIGSHVLVLSPEEHDRVVAFTSHLPQLASTALASLLASTLHSAEDRLVAGPGLQDAT
                     RLALSDYEIWSDILQTNTGNIRAALREYIDRLRQFEES"
     gene            complement(779..1792)
                     /gene="aroF"
                     /locus_tag="VFQ79_14225"
     CDS             complement(779..1792)
                     /gene="aroF"
                     /locus_tag="VFQ79_14225"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU0140875.1"
                     /translation="MLVVMQEGATEPQIQAVIDRLVARGFAIHRSTGVVHTVLGGVGP
                     MESFDPRDFEVMDGVKECHRIMSPYKLASRSFRPAGTVVKVGDIEIGGGKVVVMAGPC
                     SVESAEQISRAAELVAKAGATVIRGGAFKPRSSPYSFQGLGEEGLLLLRKAADDNGLH
                     VVSEVMDRSQIPLVAEYADILQVGARNMQNFNLLRELGNVRKPVLLKRGIAATIEELL
                     LSAEYIMAGGNYDVILCERGIRTFESYTRNTMDISAIPIVKKLSHLPMIADPSHGTGR
                     RDKVLPMARAAVAAGADGLIIEVHHDPDHALSDGAQSLRPEQFTLLMEQLRIIAPAVG
                     RTV"
     gene            complement(1811..2131)
                     /locus_tag="VFQ79_14230"
     CDS             complement(1811..2131)
                     /locus_tag="VFQ79_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU0140876.1"
                     /translation="MNREEGMRQLAACRDRIDDVDRRLITLLNERTRIVDEIGELKRK
                     LQLPIYEPRREDEVYRNVVKSNSGPLSPEALKRVFERIIDEMRTVQREKMLKEDAQAG
                     TPPE"
     gene            complement(2128..2931)
                     /gene="trpA"
                     /locus_tag="VFQ79_14235"
     CDS             complement(2128..2931)
                     /gene="trpA"
                     /locus_tag="VFQ79_14235"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU0140877.1"
                     /translation="MTRIPALFERLKQENRSGLIAYITAGDPCPERTPSLIAALEEGG
                     VDLVELGVPFSDPVADGPVIQRASERALKAGTSLARVLDIAAEVRRRSDIPIVLFTYL
                     NPVLRYGTEKLVRDAVERGIDGCLLTDLSVEEAASYVETMRRFGLDAIFLATPTSSAK
                     RCKLIAEHSTGFVYLVSRAGVTGEKDSVSATVAPLVASMRSLTNLPLAVGFGISKPEH
                     AAEVGAVADAVAIGSAFMRTIEQHRDDALEQRLTAQARGFRDALFRRSA"
     gene            complement(2906..4117)
                     /gene="trpB"
                     /locus_tag="VFQ79_14240"
     CDS             complement(2906..4117)
                     /gene="trpB"
                     /locus_tag="VFQ79_14240"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU0140878.1"
                     /translation="MISEPDSQGHFGPYGGRYVPEILMSPLEALEQAWEEARHDPAFH
                     SELNELLETYAGRPTPLYFARRLSREAGGARIYLKREDLLHTGAHKINNCLGQVLLAR
                     RMGKTRIIAETGAGQHGVATATVSALFGMECVVYMGEEDMRRQRLNVFRMRLLGAAVV
                     GVSSGSRTLKDAISEAMRDWVTNVSNTHYLLGSALGAHPYPLMVRDFHKVIGEETRRQ
                     VMEREGRLPDAVFACVGGGSNAIGMFYPFVEDEGVRLVGVEAGGRGAALGEHAARFSG
                     GLPGVLHGARSYLLQDSDGQISLTHSISAGLDYALVGPEHAWLHDRRRAEYVSITDTE
                     ALDAARTLSRMEGIIPALESSHAVAEALRRAPAEKDRVFVVNLSGRGDKDMDIYRENL
                     KELDDANSRAI"
     gene            complement(4114..4719)
                     /locus_tag="VFQ79_14245"
     CDS             complement(4114..4719)
                     /locus_tag="VFQ79_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU0140879.1"
                     /translation="MIVKICGLTNREDALAAAEVGATALGFNFFAGSPRYVRPEAAAR
                     IIEELPPSVWTVGVWVNEDPEQILALSVSHGIQVAQLHGDEGPDSVPSGIRVWKAVRM
                     GAASGKIDRESRQVEALVLDTAAAGLYGGTGQTFDWSIARSVPGKVILAGGLDASNVR
                     EAIRTAKPWGVDAASRLEIAPGRKDHVKMSEFIKAALEETE"
     gene            complement(4716..5501)
                     /gene="trpC"
                     /locus_tag="VFQ79_14250"
     CDS             complement(4716..5501)
                     /gene="trpC"
                     /locus_tag="VFQ79_14250"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU0140880.1"
                     /translation="MTGALPDILARIADRKRSDLLSASERLPELECEADRMVARRRDF
                     RRALVETKPAIIAEIKKASPSKGVLSAAFEPAGIAATYERGGAAALSVLTDEPFFQGS
                     LHDLETARKATALPVLRKDFTLEKFHVVEAAAHGADAVLLIAALHTRDSLRELRELAS
                     RYRMSTLVEVHDEEELDVAVESGAEIIGVNNRDLRTFQVFLETSLRLAPRIPADIIKV
                     SESGIDSAADVRRLLDAGFNAFLVGEHLMRAADPAGALRAFLA"
     gene            5744..6172
                     /locus_tag="VFQ79_14255"
     CDS             5744..6172
                     /locus_tag="VFQ79_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140881.1"
                     /translation="MVHRFPTHSMGLASPAEVDTVEQEHIFPRSWSWRGIKEWFKAQG
                     APACKVTAVCIPPGARLMVHDIPENIQQEFGVRPTEEVVFVELTSEAFTYRDGVRFGN
                     GRQVLLQRFRAGQRVQVLRLGSSEDTPRPEETIEPAWTRR"
     gene            6295..7134
                     /locus_tag="VFQ79_14260"
     CDS             6295..7134
                     /locus_tag="VFQ79_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0140882.1"
                     /translation="MYTRRQFTTIALATIPVSAAAKPGSTGVMIGAQTYSFRDRSLSG
                     AIEGMKETGLGYAEVWSGHIEPKDLSREELRKWRTTVSMGEFKKVRRQFEAAGIQLYA
                     YNYSFRDDFSDEEIARGFEMARALGAKCLTASANVSTARRVDPFANKAKIVVGMHNHS
                     RIRPNEFARPEDFFDAMKGMSRYIGINLDIGHFWGAGYDPVSFLKEHHSRIVTLHVKD
                     KKREGDVNMPFGQGDTPICDVLRVLRDQKLRIPAMIEYEYKGSDAVEEVKKSFDFMKR
                     CIA"
     gene            7260..>7685
                     /locus_tag="VFQ79_14265"
     CDS             7260..>7685
                     /locus_tag="VFQ79_14265"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0140883.1"
                     /translation="MNRASLVALLTIFMLAVAMADIASAQVLYGSAVGTVEDQTGALV
                     SGASVTMTNTSTGLVREDKTGANGRYSFTNLLPGRYDVKVVAAGFRTYTQTNVEITIN
                     TVTRVDVKLELGQVTEQITVEASAAALQTDKSDVRSEITS"
BASE COUNT         1542 a         2421 c         2255 g         1467 t
ORIGIN      
        1 gattcctcga actgccgcaa ccggtcgatg tattcgcgca aggccgcgcg aatattgccg
       61 gtattcgtct ggaggatatc gctccagatc tcgtagtcgc tcagggccag gcgggttgcg
      121 tcctggaggc ccggtccggc caccagccta tcttcagccg agtgcagagt gctggcaagc
      181 agcgatgcca gcgccgtcga agcaagctgc ggcaaatgag acgtgaaggc gaccactcta
      241 tcgtgctcct cgggggagag caccaaaacg tgtgagccga tgcgttccag ccagcgccgg
      301 aactcgcgga ccgcatcggt gtccatttcg tccggttgcc gaggcgtaag caaataggta
      361 cgcccggcaa acagcgctgc atccgccgcg ccaacgcctc gaacttcctt gccggccatc
      421 gggtgtccac ccaggaactg agcgctccgg atggcggccg cggcttccac aatctcccgc
      481 tttgtgctgc cggcgtcggt gatcagggca cccggtttca ggcagccttc gagtaaagcg
      541 atcgtctcca gaatcgcccg gatcggctgc gcgagataga gaagatccgc ccggtgcgcc
      601 gcatcgctca gagtcgacgg ctcatcgata gcttcgagct ccagcgcctt atccagcgtt
      661 ttgcgggagc tgacgccgag gattgtgccg gcaaacccgg cttttcgaag ggccagagcg
      721 aaggagcctc caatcaggcc caccccgatg atggcgacgg tctgcatgaa aggggaagtt
      781 atacggtccg gccgacggcc ggcgcgatga ttcgcaattg ttccatcagc agcgtgaact
      841 gctccggccg cagcgactgc gctccgtcgc tcaacgcgtg atcgggatcg tgatgcacct
      901 cgatgatgag accgtccgcg ccggccgcaa ccgccgcgcg agccatgggc agcactttgt
      961 cgcggcgccc ggttccgtgc gaaggatcgg cgatcatcgg cagatgcgac agctttttca
     1021 caattgggat ggccgagatg tccatcgtgt tccgtgtata gctctcaaac gtgcgaatcc
     1081 cacgctcgca gagaatcacg tcgtagttgc cgccggccat gatgtattcg gccgatagca
     1141 gcagttcctc aatggtggcg gcgatgcccc gtttcagcag aacgggcttg cggacgttcc
     1201 cgagttcccg gagcagattg aagttctgca tgtttcgcgc ccccacctgg aggatgtcgg
     1261 catactcggc caccagcggg atctgagaac ggtccatcac ttcgctgaca acatgcagcc
     1321 cattatcgtc ggcggcctta cgcaacagca gcagtccctc ttcgccaagc ccctggaaac
     1381 tgtaaggaga actgcgtggc ttaaaggcgc cgccccggat gaccgtggcg ccggctttgg
     1441 cgacaagttc agcggcacgc gaaatctgct ccgcactttc gacactgcac ggccccgcca
     1501 tcacgacgac cttgccgccg ccgatctcta tatcgcccac cttcaccacg gtgcccgccg
     1561 gccggaaact gcggctggcg agcttgtagg gcgacatgat gcggtggcat tctttaacac
     1621 cgtccatgac ctcgaagtct ctcgggtcga aactctccat cgggccgacc ccgccgagga
     1681 ccgtgtgcac cacaccggtg gagcggtgaa tcgcgaaacc ccgcgccacc agccggtcga
     1741 tcacagcctg aatctgcggc tcggtagcgc cttcctgcat tacgactaac atgacgttct
     1801 cctggcaggc ctactcgggt ggagtaccgg cctgcgcatc ctccttcagc atcttctctc
     1861 tttgtacggt gcgcatttcg tcgatgatgc gttcaaaaac acgcttcaat gcttccggag
     1921 agagagggcc ggaattgctt tttaccacat tccggtatac ttcatcctcg cgcctgggtt
     1981 cgtagattgg caattgaagc ttccgtttca actcgccgat ttcgtcgaca atgcgggtgc
     2041 gctcgttcag tagagtaatg agccgccggt ccacgtcatc aatgcgatcc cggcacgccg
     2101 caagctggcg cattccctct tcgcggttca tgcggatctc cggaaaagag cgtcccgaaa
     2161 gcctcgcgcc tgcgcagtca gacgttgctc cagcgcatcg tcgcggtgct gctcaatcgt
     2221 ccgcatgaac gcgcttccaa tagccaccgc gtccgcgacg gcgcccactt cggcggcgtg
     2281 ctccggcttt gaaatcccga agccgacggc cagagggagg tttgtcagcg atcgcatgga
     2341 cgcgaccagc ggcgccacag tggcggaaac cgagtccttc tctcccgtca cgccggcgcg
     2401 cgaaaccagg tacacgaaac cggtggagtg ctccgcgatg agcttgcaac gtttcgcact
     2461 gctggtggga gtagccagga aaatcgcatc cagtccgaaa cggcgcatcg tttcgacgta
     2521 ggaagccgcc tcctcaacgc tcaaatccgt caacaggcag ccgtcaattc cgcgttccac
     2581 ggcatcgcgc acaagcttct cggttccgta acgcaagacc ggattcaagt acgtgaataa
     2641 aacaatcgga atatcggagc gccggcgaac ctccgccgct atatcgagca cgcgcgccag
     2701 gcttgttccg gccttgagcg cacgctccga agcccgttgg ataaccggcc cgtccgccac
     2761 cggatcggaa aacggaacgc cgagctccac caggtccacg ccaccctctt caagggcggc
     2821 tattagcgag ggcgttcgct ccggacaagg atctccggcc gttatgtagg caatcagccc
     2881 ggagcggttc tcctgtttga gccgttcaaa tagcgcggga attcgcgtca tccagttcct
     2941 tcagattctc ccggtagatg tccatgtctt tgtcgccgcg ccccgagaga ttgacaacga
     3001 aaacgcggtc cttttccgcg ggtgcgcggc gcagggcttc agccacggcg tgcgacgatt
     3061 cgagcgccgg gatgatgcct tccatgcggg aaagcgttcg cgccgcgtcg agcgcctccg
     3121 tgtcggtgat cgagacgtat tcggcccgtc gccggtcatg cagccaggcg tgttccgggc
     3181 cgaccagcgc atagtcgagc cccgcggaga tggaatgcgt cagtgagatc tgcccgtcgg
     3241 agtcctgaag cagatagctg cgggcgccgt gcagaacgcc aggcaacccg ccggaaaatc
     3301 gcgccgcatg ctcccccagt gcggcaccgc gccctcccgc ttcgacacca accagacgaa
     3361 ctccctcgtc ctcgacgaac ggatagaaca taccgatcgc gttgctgccg cctccgacac
     3421 aggcgaaaac ggcatcgggc aaacggcctt cgcgttccat cacctgccgc cgcgtctcct
     3481 cgccgatcac cttatgaaaa tctctgacca tcagagggta cgggtgagcg cccagcgccg
     3541 aacccagcag gtaatgggtg ttcgaaacgt tcgtgaccca atcgcgcatc gcttcactga
     3601 tggcatcttt gagtgtgcgg ctgcccgagg aaacgccgac gacggccgcc cccagcaaac
     3661 gcatgcggaa gacgttcagc cgctggcgcc gcatgtcctc ctcacccata taaaccacgc
     3721 actccatgcc aaacagggcc gacacggtag ccgtggcgac gccatgctgt cccgcaccgg
     3781 tttcggcgat aatgcgcgtc ttccccatgc ggcgcgcgag cagaacctgc ccgaggcagt
     3841 tgttaatttt atgcgcaccg gtgtgcagca ggtcttcccg cttgagataa atgcgcgcac
     3901 cgcccgcttc ccggctcaaa cgccgcgcga aatagagcgg cgtcgggcgg ccggcgtaag
     3961 tctcgagcag ttcgttcagc tcgctatgga aagccggatc gtggcgcgcc tcttcccagg
     4021 cctgctccaa cgcttccagc ggagacatga gaatctcggg aacatagcgg ccgccgtacg
     4081 gtccgaagtg gccctggctg tcgggttcgg aaatcactcc gtctcctcca gggctgcttt
     4141 tatgaattcg ctcatcttca cgtgatcttt tcttccaggc gctatctcaa gacgggaggc
     4201 cgcatcgaca ccccatggtt tcgccgtacg gatcgcctcg cggacgttcg aggcatccag
     4261 gcctcctgcg aggatgacct ttccaggtac gctccgggcg atgctccagt caaatgtttg
     4321 tcccgtgccg ccgtaaagac ccgcggccgc ggtatcgagc accagcgcct ccacctgccg
     4381 gctctcgcga tcgatcttgc ccgaagcggc ccccatgcgc acagccttcc agacacgaat
     4441 cccggagggc accgaatcag gcccctcgtc gccgtgcagt tgggcaacct ggatgccgtg
     4501 cgatacgctc aaagccagaa tctgttccgg atcttcgttc acccacacgc cgacggtcca
     4561 gacggacggt ggcagctctt caatgatgcg agccgcggcc tcgggccgga catatcgcgg
     4621 gctgcccgca aaaaaattga agcccaacgc ggttgcgccg acttcagcgg ctgccagcgc
     4681 atcttcacgg tttgtaagcc cgcagatctt gacaatcatg cgagaaacgc cctcagtgcc
     4741 ccggcggggt cggcggctcg catcaggtgc tcgcccacca gaaatgcatt gaaaccggca
     4801 tccagcaggc ggcgcacgtc cgcggcggaa tcgatgccgc tctcgcttac tttgatgata
     4861 tcggccggga tgcgaggggc cagccggagc gatgtttcga gaaaaacctg aaacgttctc
     4921 agatcgcgat tgtttacacc aataatttcg gctccggatt cgacggccac gtccaattcc
     4981 tcctcgtcgt gtacttcgac cagagtagac atacgatacc gcgatgccag ttcccgcaac
     5041 tcgcgcaagg aatcgcgcgt gtggagcgcc gcaatcagaa ggacagcgtc cgcaccatgc
     5101 gccgcggcct ccaccacgtg aaatttttcg agtgtgaagt ccttccgcag gacgggaagg
     5161 gccgtggcct tgcgcgcggt ttcaagatcg tggaggctgc cctggaagaa gggctcgtct
     5221 gttaacaccg agagtgccgc tgccccgccg cgctcgtacg tggcggcgat cccggccggc
     5281 tcgaacgctg ccgaaagcac tcctttgctc ggtgacgcct tcttgatctc cgcgataatg
     5341 gcgggctttg tctccaccag tgcgcgccgg aaatcgcggc gacgtgccac catccggtcc
     5401 gcctcgcatt ccaactccgg cagccgttcg ctggcggata aaaggtcgga tcgcttgcgg
     5461 tccgcaatgc gagcaaggat atcaggtaaa gccccggtca tttgtaggat tctttatgct
     5521 agcatgtcgg gcacggggcg ccggttctct tgtccgggag cgccggccgg taacacatgt
     5581 tgtacgtttc cacatcatcc ggcaggaacg cattagcccg acacgaaagt ctcatccccg
     5641 gcgtccaaac ttacgacaga ggctcagagg aggttttaag ctaatgtgtg atttctcgct
     5701 gcacgcaaat cccaaccgac tcgccagaga aggggagcag cttgtggtac accgtttccc
     5761 aacgcattcg atgggcctgg cctcgcctgc cgaggtcgat accgtcgagc aggagcacat
     5821 ctttccaagg tcctggtcct ggcgtggaat taaggagtgg ttcaaggcac agggcgctcc
     5881 ggcgtgcaaa gtgaccgcgg tttgcatccc gccgggtgcg cgcctgatgg tccacgacat
     5941 tccggagaac attcaacagg aattcggcgt gcggccgacg gaagaggtgg tttttgtcga
     6001 actgacaagc gaggccttca cctaccggga cggcgtacgc ttcggcaacg gccggcaggt
     6061 actgctgcaa cgtttccgcg ccggacaacg agtgcaggtg ctgcggctgg gttcatcgga
     6121 agataccccg cggcccgagg aaacgatcga gccggcctgg accagacgct gacagcgcgt
     6181 gtggttcatt cccttcccgg ggctcgctgg acgcccgatg ttacggtccg gccgcgttag
     6241 cagtagagag ggttcgacgg tgatatgctg ggtcctttca gcaaaggagg atctgtgtat
     6301 acccgccgcc agttcactac gatcgcctta gccaccatcc ctgtttccgc ggcagcgaag
     6361 cccggctcca cgggtgtcat gattggagcc cagacctaca gcttccggga ccgtagcctt
     6421 tccggcgcta tcgaagggat gaaggaaacg ggcctcggct atgcggaagt gtggtccggc
     6481 cacatcgaac ccaaagatct ctcccgtgag gaattgcgca aatggcggac caccgtttcc
     6541 atgggcgagt tcaagaaggt gcgccggcag tttgaagccg ccggtattca actctacgcc
     6601 tacaactaca gcttccgcga cgacttttca gatgaggaga tcgcgcgcgg attcgagatg
     6661 gcgagagctt tgggagcgaa gtgccttacc gcttcggcca acgtgagcac cgccaggcga
     6721 gtggacccgt ttgcgaacaa ggcgaagatc gtggtcggca tgcacaacca ctcgcggatc
     6781 cgcccgaacg aattcgcccg ccccgaggat ttcttcgatg caatgaaagg catgtcgcgc
     6841 tacatcggta tcaatttaga tatcggacat ttctggggcg ccggttacga cccggtatcg
     6901 ttcctgaagg agcatcactc gagaatcgtc accctgcacg taaaagacaa gaagcgggag
     6961 ggcgacgtga atatgccctt cggccagggc gacaccccca tctgcgacgt gctccgtgtg
     7021 ttgcgggacc agaaactccg catcccggca atgatcgagt acgaatataa aggaagcgac
     7081 gcagtagaag aggtcaaaaa aagtttcgat ttcatgaagc gctgcatcgc gtaagaaaaa
     7141 tcctgacacg cccgaaaatc ctgattgact tccccccatc gccgtgatag cgtcttttcc
     7201 cataactcgt tgttctcggt gtttagaagc cagactgcta gttcttgggg aggaaagaaa
     7261 tgaatcgagc cagcctcgta gctctgctta caattttcat gctggcggta gcgatggccg
     7321 atatcgcgtc cgcacaggta ctgtacggtt ccgcggtagg aaccgtcgaa gatcaaaccg
     7381 gcgcactcgt atcgggcgcc tcagtcacga tgacgaatac gtccacaggt cttgtcaggg
     7441 aggataagac cggggcgaat gggcgctaca gctttaccaa tctcctgccg ggccggtacg
     7501 atgtcaaggt agtcgcggcc ggatttcgca cttacacaca gaccaatgtc gaaatcacca
     7561 tcaacacggt tacgcgcgtg gacgtcaaac tcgaactcgg acaggtaact gagcagatta
     7621 ccgtggaagc aagcgcggct gcgttgcaga ctgacaagtc ggatgtgcgg tcggaaatca
     7681 cgagc
//
LOCUS       DASTMK010000329        21051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_624012, whole genome shotgun sequence.
ACCESSION   DASTMK010000329 DASTMK010000000
VERSION     DASTMK010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21051
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624012"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VFQ79_14270"
     CDS             complement(<1..1468)
                     /locus_tag="VFQ79_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140884.1"
                     /translation="MRHLLPLLLLLPLAVYAQAPAAEPAQEAKPAEPTTEAKPAETEA
                     EAKPAEPAAEAAAESPAPETERWITGTLDFGYRWVSDIGGNFNTYRSVIDLGEGPKLF
                     GLDLTLQPASSRLLDRLDIFANNWGGEPYTTARIFARKQRAYQLSFDYRNIDYFNFLP
                     SFANPLAGQGGLISQRAFDTRRRTIDTELELFPGARIVPYVAFTRNSGDGSGITPFQT
                     DSNEYPVPTRLRDETNNYRGGVRFEMNRWHVTLEQGGLTFKDDQRVFTDQRNLGNRTT
                     PLFGEQLFIDGLEQAYGVRGSSIYSKGLVTASPVSWANLYGQFLFSQPTTDVNYGASA
                     SGLLTILAARRFYTGQQEFVTSESRMPHISGSLSLEIRPLDRLRVLGSWMTDRFHNAS
                     SISLTDVFLFATSPEEVERAFATDRLDVNYNRSQIDAFFDVTRKITLRGGYRYVWGDA
                     TARAPQLNPIATREQGEISRHVGIAGFNIRPSQNLSANF"
     gene            complement(1465..2505)
                     /locus_tag="VFQ79_14275"
     CDS             complement(1465..2505)
                     /locus_tag="VFQ79_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEU0140885.1"
                     /translation="MRFANAHHALSLRRRKARLTAGLSAAGITLAGCLLALPGLRGQA
                     QPEQQPAQEQAQTTEQAQTPPEPATYIGSETCQACHEDIFKAFQKNPHAAVEKDSKRG
                     WEGKSCESCHGPGSKHAESASADDIINPGKLHPAKADQNCLKCHLNQPTQAGRIHGGH
                     ARNQVSCVGCHPVHQTTTSGMVPRKVDAINQQCSSCHTSVWAQFQRPHAHRLPQGAMS
                     CVDCHNPHGSFLPSSVRTVSANEPGCMKCHSDKRGPFAFEHPPVRLEGCSTCHEPHGS
                     ANPRMLTRHEVRFQCLECHSNIAMPVAANQPGTLGGTPPAFHDLRSPRFRNCTVCHMK
                     IHGSHVNRALLR"
     gene            complement(2534..4588)
                     /locus_tag="VFQ79_14280"
     CDS             complement(2534..4588)
                     /locus_tag="VFQ79_14280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HEU0140886.1"
                     /translation="MRLRIPLGWGVRFALVGAVFAGCVALVSAPKPVYSPKEKAAYAD
                     PSLVNFVRPGLVVKVLGAEIAQDGTIKARVRFTDPAGLPLDKDGVTTPGRISNGNPGM
                     IAAYIPKDQRQYVAYTTRTQRSTITGRSAIQASGDTGGSWQKVAEGEYVYTFGTRAPA
                     SIDRTTTHAIGIYANRNLTEFELGTNLDDDVLDFVPDGSPVTKVRDIIRTESCNDCHS
                     RMAFHGSTGRISVPMCNLCHTPQTIDPDTDNTVDMPVMIHKIHAARDYVIVGNANASH
                     DYSEVTFPTDLRNCSVCHEEDSPAVQKDAWLTQPTRAACGACHTDVNFATGAGHVNLP
                     QISDNQCSNCHVPQGELEFDASIRGAHTVPQRSNMLSGLQFEILDVADGQAGKKPTVT
                     FAVKDKNGNPVELSRMGRLALTLTGPTTDYTAFENGFIQEDAITAQGSGGRLFWTFST
                     AIPENATGTWAIGIEGRRVETLLEGTQKQMSVQYGAPNVMHYFAVDGSQVQPRREIVS
                     IQKCNECHVRLALHGENRVDNVQYCVVCHNPKETDVSVRPAGQNPPESINMATMIHKI
                     HGGRELHGQFGSEFVVYGRGGTPHNYSEVTYPAPLSDCNMCHVNDSQSLPLPLTNADV
                     NNPRGLINPAPPATAACTSCHQSIAAASHALANTTRLGESCAACHGRNSDFSVNKVHA
                     SQ"
     gene            4875..5384
                     /locus_tag="VFQ79_14285"
     CDS             4875..5384
                     /locus_tag="VFQ79_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0140887.1"
                     /translation="MDWNTTSGGAAIEGSGVALQQLPDPLREAGKKSVLLVSPFDEDV
                     RFIRDCLPDDAPHLDTAENYTRAAGLLQANSFSVVLSERDLPDGGWRDVLNAVELCQS
                     TPLLIVASRHADERLWAEVLNLGGFDVLMKPFDFMETTRVLAMACQQWGSGSYGAMSR
                     GSREHGPGW"
     gene            5413..6273
                     /locus_tag="VFQ79_14290"
     CDS             5413..6273
                     /locus_tag="VFQ79_14290"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEU0140888.1"
                     /translation="MSSKTIAVAGGGRVACALGRFFQKRGYTVSAVASRSRKRASAAA
                     ASISPTTTATRYADLPRHATHILIAVSDVAIPEVARMLAVPAMRGGVALHTSGTRGVE
                     ALQPLSDAGAHCGSLHPLQTISDLERSVLALHGAAFAIDGDETALAWALELVRAAEGR
                     PLRIAENDRAAYHAAAVLAGNYLITLLDRAAGVFESIGIPRGQALLALAPLARASLEN
                     AVMDGPERALTGPISRGDVETVQAHLAILPPDVRDLYRICGLATIEVARRQGLSGEAA
                     VALERVLRQN"
     gene            6279..7118
                     /gene="panB"
                     /locus_tag="VFQ79_14295"
     CDS             6279..7118
                     /gene="panB"
                     /locus_tag="VFQ79_14295"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU0140889.1"
                     /translation="MAEKPIKIRVPELLEMKKRGEKIAMLTAYDATMARILDRAGVDV
                     LLVGDTLGMVIMGENTTLPVTLDVMVHHTRAVANGARRALVVADMPFLSYQLGEVEAV
                     RNAGRLIQEGRASAVKLEGGLVVAPVVQRLVETGIPVMGHIGLIPQSVHMLGGFKPVG
                     ARPAEAEHLLEEAAALQEAGAFSVVLEWMPPEVARKITQSLEIPTIGIGAGPHCDGQV
                     LVNYDAFGLFEEFTPPFVKRYAELGAEMSKAAEQYIADVREGRFPTEEHTYRAAAAKA
                     RPK"
     gene            7115..7969
                     /gene="panC"
                     /locus_tag="VFQ79_14300"
     CDS             7115..7969
                     /gene="panC"
                     /locus_tag="VFQ79_14300"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU0140890.1"
                     /translation="MKPRILKGIPETRTALAGAAGLGLVPTMGALHDGHGSLIEVARR
                     VSAVVITSIFVNPTQFNQASDYAQYPRDLAADVEFCAARGVDFVFVPEAAEMYPEPPL
                     TTVEVAQIGDGLCGRYRPGHFRGVATVVAKLFHIIQPERAYFGEKDAQQLALIRRMTA
                     DLNMPVEIVPVPTVRDADGLALSSRNRRLNSRERAAAPVLYQALSAAAARVAAGETDP
                     GKVKAVALETLGREPLVRVEYLELVDPERMQAVDRIDGPVRAAAAIWLGETRLIDNVL
                     SQPVQIPR"
     gene            8008..8796
                     /locus_tag="VFQ79_14305"
     CDS             8008..8796
                     /locus_tag="VFQ79_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0140891.1"
                     /translation="MDLEKALLRKVGEAIGRFRMIRDGDRIAVALSGGKDSLTLLHAL
                     LLLEKRAPVDFSVCAFTVEQGKFLRPVEPLGDYLRSRGVAWTYYHDKPSFRLLDEQPD
                     HGCDLCSRYRRRAVYEIARGLGANVIAFGHTADDFCEAFLRNTLFTGRLSALPPVAHS
                     RDKEFRLIRPLLFVTEDLTKACAESLGAPVVPCGCSQKTGTVRRSLRDFFGELERDYP
                     NLKETMLAAMGNIELHRLLDPRFIEAEPGAKETRQELFPIVMES"
     gene            8877..9491
                     /locus_tag="VFQ79_14310"
     CDS             8877..9491
                     /locus_tag="VFQ79_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU0140892.1"
                     /translation="MRFLPREEKFFALLLNQANIILEAASALLAGAQAGNAHLAECAA
                     RIVKLEQQGDEVIHDLFLRLNQTFITPLDPEDIHSLASHLDDVLDGIEDAIHRMVAYR
                     VEPIPPEVVELCGIVHACAQSLQSAFEALERDEKLLQHCIEINRQEEKADHLVRRAVE
                     ELFEHEKDPIKLIKLKEIYEFLEDTTDRCEDVADVLQNVVVKNS"
     gene            9500..10528
                     /locus_tag="VFQ79_14315"
     CDS             9500..10528
                     /locus_tag="VFQ79_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU0140893.1"
                     /translation="MTATLVLVIAIVVIALIFDYFNGYNDAANSIATIVATRVLTPFQ
                     AVAWAAFFNFVSAFSFGTGVAKTVGAGMVDLDSVTPYVIMAGLIGATLWLATTSFLGL
                     PVSASHALFGGYAGAAIARAAYVKGVSNMFDVIVWSGWIKTIVFMVVAPLLGLVLAYL
                     LMTLIYWLFQNSTPKTMDNYFRKLQLVSAAIFSYSHGTNDAQKTMGIITSALVTGGYL
                     ATFEVPIWTIFAAHSAIALGTLMGGWKVVHTVGARVTRLKPRGGFSAETAGAISVLLA
                     TYLRIGVSTTHIITGAIAGVGSFRRLKAVRWGVAKKILWAWILTIPASAAVGWLCFHL
                     FHMFVPNA"
     gene            complement(10554..12158)
                     /locus_tag="VFQ79_14320"
     CDS             complement(10554..12158)
                     /locus_tag="VFQ79_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140894.1"
                     /translation="MPSYARAILWAQWRVLRNSVSRGRGVRMYLVAAVAVLWYGMWAL
                     AAASIAILLSAPSPAFDLAVVLSNGLLLATVYWQAAPLLMASTGASLDISRLLVYPIP
                     RRELFLLELVLRALTGFEVLLLIAGAAVGLFLNPRIAWWTSLALALYVAFNLFLSAGL
                     KSALVRFTAKRRIRELVVLVMMLVVALPQYILWKGVPEPLRRQFEETHSVALPWAAAG
                     RLAAGEVSVPAWLSLLGWIVAAFFFGRRQFEGGLRSQAGTAARRVHASRMDALSRTVF
                     DLCSHFLRDPTGALVEKELRTLARAPRFRLVFVMGFSFGLLIWIPMLFGGRHAQDSFL
                     TQHYLTLVCLYALLLVGEVLFWNSLGFDRAAVQLYYVTPVRPRAVLLAKNIVAAVIVL
                     LEATAVVVVCMALRFPASASTIAEAYAVTLVYSVFLMADGNISATRHARPMNPEHSWR
                     SSSGSRGALLLVFYPLLFVPVSLAFAARFAFASETAFWVVLFLIAVFAGIVYRIALES
                     GVGALQRNRERLIASLSHSPGLIAGG"
     gene            complement(12163..12915)
                     /locus_tag="VFQ79_14325"
     CDS             complement(12163..12915)
                     /locus_tag="VFQ79_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0140895.1"
                     /translation="MNGAAIDIRDLRKTYGGQTAVDGLTLTVPRGSFFGFLGPNGAGK
                     TTTIRMLTGLAQPDAGSVELLGMSMPTQEMEIKRKMGLVPDESLLFDHLTGGEFLELV
                     GRLYGLDRKVARERASELMEMFDLGESRRKLIAEYSKGMRKRVAMAGALIHKPELLLL
                     DEPFEGVDAVGARFMKELLIEQVRRGTTIFLTSHVLEVVERLCDRIGIIHHGRLLTEG
                     TMQDLRTGSETLEDLFVRLVGGTRASEPLDWL"
     gene            complement(12924..14063)
                     /locus_tag="VFQ79_14330"
     CDS             complement(12924..14063)
                     /locus_tag="VFQ79_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEU0140896.1"
                     /translation="MHISSPPAARRGFLRRLLTLGGFSAAAPAASLAQIQPARAPQFD
                     YLPRYARALHYQSWKQSSFDQTGGNSDRWPIKPGETLEIYNQAGPGVISHIWFTIAAA
                     GRNHLKELVLRAYWDDSSKPSVEAPIGDFFGLNLGQYEIYESAYLACSPLRSLNCYFS
                     MPYRRRARMTVENQGADPVNSFYSNIDFMRVASLPDDIVYFHAQYRQCTPCVANKGDK
                     LNPLGAQNHVIIETKGRGHLMGATLGVLLNADGWMGEGDDMIFVDNDTKPAIVGTGSE
                     DYFLGSWNFGGRDGAIPFAHKYYGAPIIVMPERTGGRYCCYRWHGDNPVTFTRYLKHT
                     LEHGHANDRGDNWFSVGYWYQDTPYTDFPSLPPVQSRIPAVKLPS"
     gene            complement(14120..15250)
                     /locus_tag="VFQ79_14335"
     CDS             complement(14120..15250)
                     /locus_tag="VFQ79_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0140897.1"
                     /translation="MLDRRRFIVSAAASCAVGVLPAIAAAPGEEWRNRQSGMAYRKLG
                     RTGFMISRMVMGGNTIAPTNYEHVLLAMDMGLNYLDTAPAYGSGKSEEGYAQVLKSRP
                     RDSFFLNTKVSPWDLNRNKLYKDIFDSLPETEQTKVRHAAQEYIEKTRVFEPDYVGDY
                     FRGQRGEMEAAALSNAIAEKYGEKLDRGKNYKQIVLDSVHASLGRLGTDHVDILMCPH
                     GASTPAELRNYPEILEAFEILKKQGKVRHLGVSAHSDPAGILRAAVEQKHYSVAMVAY
                     SVVNHRFVDTALKAAHDAGLGVIAMKVARPVWSARRDGQQADPRRLAMIEKAFPGPLK
                     APQKAYAWALRNRHLSAVISELINAELVKENVPLAAGNAATA"
     gene            complement(15283..16449)
                     /locus_tag="VFQ79_14340"
     CDS             complement(15283..16449)
                     /locus_tag="VFQ79_14340"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0140898.1"
                     /translation="MFGRRITLFKLFGFAVRIDASWFIIAILVAWSLAVGFFPAQFPG
                     LTGRDYWLMAIIGAIGLFASIVFHELSHSLVARRYGLQMKGITLFIFGGVAEMEQEPQ
                     NARAELMMAAAGPAASIVIGFLFYLAYVAGRDAWSTFAVGVVAYLSWMNWILAAFNLI
                     PAFPLDGGRILRAALWHWKGDLMRATRIAAAFGSGFGLLLIMLSVVTLFSGNFITAMW
                     WFLIGMFVRGASQASYQQVVLKRTLEGESVRRFMKTDPITVPRSISIRELVDEYIYRY
                     HHKMFPVVSESNRLLGCVTSRDVRNVSREEWDNHTVQEISSPCSESNVIGPDTDAMEA
                     LSLMSRTGNSRMMVVENGELVAIVSLKDLLRFISTKLELEGREPPVKIPAGIGS"
     gene            complement(16512..17711)
                     /locus_tag="VFQ79_14345"
     CDS             complement(16512..17711)
                     /locus_tag="VFQ79_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEU0140899.1"
                     /translation="MRLLSALCLCAALSAAPPFVQVREGRLVAPDGKPVLLKGINLGN
                     WLAPEGYMLLLNEGAPESWWEMDALFRDLAGEDFTARFWREWRNRYITEADLAKISSI
                     GFNTVRVPMHWKLLEPDGDGWKCLDDVIAWSGKHNLWVILDLHAAQGGQTGTNIDDSW
                     GYPWLFESETAQEATIQLWRRIAERYRDEPVVLGYDLLNEPIPHFGNLPALNPKLEPL
                     YKRIVAAIREVDPHHIVILEGTQWASRFDSFGPPFDAQSMYSFHKYWTAPSREVIEEY
                     LRYAKKYNVPLLMGESGENTNEWISEFRQTLDADGIHWTFWPWKKMQTTSAPVSFPKP
                     RHWDAIVAYARLPGGVAAAEKKAPLRPSREQVHEALTDLLDKIRLEAGELNHGYLEAL
                     GFDSSGS"
     gene            complement(17778..>21051)
                     /locus_tag="VFQ79_14350"
     CDS             complement(17778..>21051)
                     /locus_tag="VFQ79_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEU0140900.1"
                     /translation="QLGATATTVTVEAGAAELQLQSESGERSMLVTNNQINNLAMNGR
                     NIFDMVKIIPGVTSEFNGEQSNRGGLDSFNINGTRSNQKQLTIDGSSNVDTGSNGASH
                     VTLNPDAIAEVKILTSNYQAEYGKAGGGTMVITTKGGSNEFHANARWFHRHEGLNANN
                     FFNNMRGRQPDGSEVSPRALYRYNYAGYQVSGPVLLPGTNFNRNKDKLFFFFGQEYYR
                     QLIPGGITNVRVPTPAELGGDFSQTLDGFGAPVVVRDPTTGQPYPGNIIPESAILPGM
                     RQALGLYPQPNLSGRYDYNFTTQASTEYPRREDIVRVDYQLSAMHRLYGRWINNDSSR
                     NEPYGSFLWGISNIAFPGGIRIEEPGWNASLNLTSALSPSLVNEFTFGPSVSKLRADG
                     RDGNISRGANGIDLPLLFPVDAATPIPDFSFCCTPNTSFTWSYLGAVPFYNSNTTLDF
                     SDNLTKVAGRHIFKFGIFVQRSRKDQPAWGNVNGQFNFNDELAGHPYAAALTGHYRDF
                     VQSSNRPRGFFRYTNVEWYAQDSFRLTSRLTLDYGLRMTWYQPQYDKNDQGAVFNPYL
                     FDASAAPRIYRATADGLAYDPANPGATLPGYLTGTLVPDSGSLTNGIGLASAGYPRGG
                     FDSRGIMWEPRFGFAWQMTGDGKTVLRGGGGIMHDRTQGNLGYNPVFENPPNVRNPRL
                     VDGRVQNLSSLGGSGILAPPTLFGAARDGFVPTIYSFSLGIQRELGWSTTMDVSYVGT
                     LSRHLVQTRNLNMIPYGTAFERWAQDPAKFPGGVVPAVEPNLPEAYSRAGYAFSGAYA
                     LDANYLRPYPGYAQIKHYTFDGSSNYNSLQVAVNRRFARGLTFGVAYTWSKSLVTANG
                     DENWTNPFDTRGYDYRLASWDRPHVLAFNYVYDLPQFSRAFGGPKWLSYLTDGYQLSG
                     ITRFSSGAPVEPALWIDPKQITGSITDWESPPLFVYVDGDVNQRTGTSKFNPAAFRAP
                     GIGVPAPWPRTYMRSGGLQNWDVSLFKNFKLGASESRMIQLRLETFNVFNQTNFRDVN
                     LGMSISNPSGSSGPVLTTNPLRAAGVGQAGDYGQYFGEYTNTYTGGGGPRVVQLGLKV
                     YF"
BASE COUNT         4241 a         6278 c         6365 g         4167 t
ORIGIN      
        1 cgaaattcgc gctcaggttc tgagaaggac gaatattgaa gccggcgata cccacgtgcc
       61 gggagatttc tccctgctcg cgcgtggcaa tgggattcaa ctgaggagca cgcgcggtgg
      121 cgtcgcccca aacgtagcga taacctccgc gcaacgtgat cttacgggta acgtcgaaga
      181 acgcgtcgat ctgcgaacgg ttgtaattga cgtccagacg gtccgttgcg aaggctcgct
      241 ccacctcttc cggtgatgtc gcaaacagaa aaacatccgt gagcgaaatc gaagaagcgt
      301 tgtggaaccg gtccgtcatc cacgacccga gaacccgcag ccggtcgagc ggccggattt
      361 caaggcttag gctgcccgat atatggggca tgcgcgattc cgaagtgacg aattcctgct
      421 ggcccgtgta gaaacggcgt gccgccagga tggtcagcaa cccggaggca ctggcaccgt
      481 aattcacatc ggtcgtgggc tggctgaaca gaaactggcc gtacaggttt gcccaggaca
      541 ccggactggc ggtgaccagc cccttgctgt agatactgct tccgcgcacg ccgtaggcct
      601 gttcgagccc gtcgataaac agttgctcgc cgaaaagcgg cgtggtccgg ttgcccaagt
      661 tccgctgatc cgtaaagacc ctctggtcgt ctttaaaggt gagcccgccc tgctccagcg
      721 tcacatgcca ccggttcatc tcgaaccgga caccgccgcg gtagttgttc gtttcatccc
      781 gcaggcgagt gggaaccgga tactcgttgc tatccgtctg aaagggtgtg atgccggagc
      841 catcgccgga attgcgcgtg aatgctacgt aaggaacgat tcttgcgccg gggaatagct
      901 cgagttcggt gtcgatcgtg cggcggcgcg tgtcgaaagc gcgctgcgaa atcaatccgc
      961 cctggcccgc cagcgggttg gcgaaggacg gcagaaaatt gaaatagtcg atattgcggt
     1021 aatcaaagga aagctggtat gcgcgttgct tccgggcaaa tatgcgcgcg gttgtatacg
     1081 gctcgccgcc ccagttattg gcgaaaatat caagccggtc cagcagccgc gaagatgccg
     1141 gttggagcgt caggtccagg ccgaacagct tgggaccttc gccaagatcg atcacgctgc
     1201 ggtacgtatt gaaattgccg ccgatatcgc tcacccaccg gtaaccgaaa tcgagagttc
     1261 cggtaatcca tcgctccgtt tcaggtgccg gagattcggc tgccgcttcg gccgcaggtt
     1321 cagcgggttt cgcttccgct tccgtttcag cgggcttggc ttcggttgtc ggctcggcgg
     1381 gtttggcctc ctgcgccggc tccgccgccg gagcctgggc atacacggcc agcggcagca
     1441 gcaataggag aggaagtagg tgtctcatcg caggagcgcc cgatttacat gcgagccatg
     1501 tatcttcatg tgacacaccg tgcagttgcg aaacctggga ctgcgcaggt catggaatgc
     1561 cggtggagtt ccgccgagcg ttcccggctg gttcgccgcg accggcatcg cgatattgga
     1621 atgacactcc aggcactgga accgtacttc gtgccgggtt aacatgcgcg gattggcaga
     1681 cccgtgcggt tcgtggcacg tactgcaacc ttccaaacgt accggcggat gttcgaatgc
     1741 gaagggcccg cgcttgtcgc tatggcactt catacagccc ggctcgttgg cagagaccgt
     1801 gcgaaccgaa ctcggcagga agctgccgtg cggattatgg cagtccacgc aggacatggc
     1861 gccttggggc agcctgtgtg catgcggacg ctggaattgc gcccaaaccg atgtgtggca
     1921 actggagcac tgctggttta tggcgtccac tttgcgcgga accattccac tcgtggtggt
     1981 ctgatgaacc ggatggcagc ctacacaact cacctggttc ctggcatgcc cgccgtgtat
     2041 ccggccggct tgcgtcggct ggtttaaatg gcacttcaga cagttctggt cggccttggc
     2101 cggatgtagt ttgcccggat taataatatc gtccgccgaa gcggattcgg catgtttcga
     2161 cccgggcccg tggcaggatt cgcaggactt cccttcccag ccccgcttgg agtctttctc
     2221 gacggcggca tgggggttct tctgaaatgc cttaaaaatg tcctcgtgac aagcctggca
     2281 ggtttccgag ccgatgtaag tggctggttc gggtggagtt tgagcttgtt ctgtagtttg
     2341 agcctgttcc tgcgccggct gctgctcagg ctgggcctgg ccccggagac ccggcagcgc
     2401 caatagacac ccggccaggg tgatccccgc agcggagagg cccgccgtca agcgggcctt
     2461 ccgtcttcgc agagaaagag catgatgcgc gtttgcgaat ctcatggtct gtcaggcgca
     2521 accgctggcg ccatcactgg gaagcgtgta ccttgttcac cgaaaagtcg gagttacgtc
     2581 cgtgacacgc ggcacagctt tcgccgagcc tggtggtatt cgccaaagcg tgagacgccg
     2641 cggcgatact ttggtgacat gacgtgcaag ccgcggtggc cggcggagcc ggattaatga
     2701 gaccccgcgg gttgttcaca tcggcattgg tcagcggcag cggcagcgat tgtgaatcat
     2761 tgacatggca catgttgcaa tccgacagcg gagccggata ggtcacttcg ctgtagttgt
     2821 gaggcgtacc gccacgtccg tacacgacga attcactgcc gaactgtccg tgcagttccc
     2881 tgccgccgtg aattttgtgg atcatcgtcg ccatattgat gctttccggc gggttttgcc
     2941 cggcaggccg gaccgaaaca tcggtctctt tcggattgtg gcagaccacg caatactgca
     3001 cgttgtccac tctgttttca ccgtgaaggg cgagccggac gtgacactca ttgcacttct
     3061 gaatggagac gatttcccgg cgcggctgta cctgggaacc gtccaccgcg aaatagtgca
     3121 tcacgtttgg cgccccgtac tgcacggaca tctgtttctg tgtgccctcc aggagagttt
     3181 ccacgcgccg gccctctatg ccaattgccc acgtccctgt agcgttctcg gggatcgcgg
     3241 tgctgaaggt ccagaataac cttccgccgc tcccctgcgc cgtgatggca tcttcctgga
     3301 tgaagccatt ttcgaaggct gtgtaatcgg ttgtcggccc ggtcagcgtt agcgcgagcc
     3361 gtcccatcct ggaaagctcg accggattgc cgttcttgtc tttgaccgca aacgtcaccg
     3421 tcggcttctt gccggcctgg ccatcggcca catccagaat ttcgaattgc aggcccgaaa
     3481 gcatattaga gcgctgtgga acggtgtgcg cgccccggat ggaggcgtcg aattccagtt
     3541 cgccctgcgg cacgtggcaa ttcgagcact gattgtcgga aatttgcggc aggttgacgt
     3601 gccccgcgcc ggtggcgaaa ttcacgtccg tatggcaggc gccgcaagcc gcgcgcgtcg
     3661 gttgcgtgag ccaggcatcc ttttgaaccg ccggcgaatc ctcttcgtgg cagacggagc
     3721 agttccgcag atcggtcggg aacgtaactt ccgaataatc atgggacgca ttggcgttgc
     3781 caacgatgac gtagtcccgg gcggcgtgaa tcttgtggat catcaccggc atgtccacgg
     3841 tattgtcggt gtccgggtcg atcgtctgcg gcgtgtggca caggttgcac atcggcacgc
     3901 tgatccggcc cgtcgacccg tggaaagcca tccgggagtg gcaatcgtta cagctttcgg
     3961 tgcggatgat gtcgcggacc ttcgtcaccg gcgaaccgtc gggtacgaaa tccagaacgt
     4021 catcatccag gttcgtgccg agctcgaatt cggtaagatt gcggttcgcg tagatgccga
     4081 ttgcgtgcgt cgtcgtgcgg tcgatggagg cgggcgcgcg ggttccgaat gtatagacgt
     4141 attcgccctc cgccactttt tgccagctac cgccggtatc gcctgaagcc tgaatcgccg
     4201 accggcccgt aatggtgctc cgttgcgtgc gggttgtata ggctacgtac tgccgttgat
     4261 ctttcggaat ataagccgca atcataccgg gattgccgtt gctgattcgg cccggtgtcg
     4321 taactccatc tttatcgagc ggcaagccgg cgggatcggt gaagcgaact ctcgccttga
     4381 tcgtgccgtc ctgggcgatt tccgccccca gaaccttcac tacaagaccg gggcgaacga
     4441 aattcaccag cgacggatcg gcataggccg ccttttcttt tggggaatat accggttttg
     4501 gtgcactgac caaagcaaca cagccggcaa ataccgcccc taccagggcg aagcgcactc
     4561 cccaacccaa tgggattcga agacgcataa aattacacac tcctgctttg cgcaagctaa
     4621 ataaacgcag cttctacaaa tgcaacttcg gcgcagacca atgcacgact gggtccatcc
     4681 tctgctccag ccttaaatta ctgacagcaa atgcgattcc agcaggcggc tgcgccatag
     4741 aagatattct agtctgattt gggtgaaatg gctacaaaaa aatgtgtcat atgccacaaa
     4801 gcccgatctt ggccccctgc ttgcccaccc tccaggcaag tgcggagtgc cggggttcga
     4861 aaggcacggt tcttatggac tggaatacta cgtcaggagg cgcggctata gaggggtctg
     4921 gcgttgcgct gcaacaatta ccggatccgt tgcgcgaggc tggaaaaaag tcagtattgc
     4981 ttgtctcgcc gttcgatgag gacgttcgct tcatccggga ctgcttaccg gacgacgcac
     5041 ctcacctgga taccgcggag aactacactc gggccgccgg actccttcaa gcgaactctt
     5101 tctcggtagt tttatccgag cgtgacctcc cggatggcgg ttggcgggat gttctgaacg
     5161 cagtggagct ctgccaaagc accccccttc tgattgtggc ttcccggcat gcagacgaac
     5221 ggctgtgggc ggaggtattg aacctgggcg gcttcgacgt gctcatgaaa ccgttcgatt
     5281 tcatggaaac gacccgcgtc cttgccatgg cgtgccagca gtggggctcc gggagttacg
     5341 gcgcgatgtc gagagggtcg cgagaacacg gacccggttg gtgaagccat cggggtgtga
     5401 gaaaatgccc cgttgagcag caagacgata gccgttgcgg gcggggggcg cgtggcatgc
     5461 gcgctcgggc gtttttttca aaaacgcggt tataccgtaa gtgccgtcgc cagccgctcc
     5521 cgcaaacgtg cttctgcggc tgcggcctcg atttcgccta caacaaccgc taccagatac
     5581 gccgatctgc cgcggcacgc aacgcacatt ctcatcgcgg tttccgatgt ggcgattccg
     5641 gaggtcgcgc ggatgctggc agtgccggcc atgcgcggag gcgtcgcgct acataccagc
     5701 ggcacacgcg gcgtggaggc gctacagccg ctgtccgatg ccggcgcgca ttgcggttcg
     5761 cttcatcctc tgcaaaccat ttccgatctc gagcgaagcg ttttggcgct tcacggagcc
     5821 gcttttgcca ttgacggcga tgaaaccgcg ctcgcctggg cccttgaact cgttcgtgcg
     5881 gcggagggcc ggccgctgcg cattgccgaa aacgaccgcg ccgcctatca tgcagcggcg
     5941 gtactggccg gtaactatct gattaccctg ctggatagag cggccggcgt ctttgagagc
     6001 attgggattc cccgcggtca ggcactgctc gctcttgcgc ctctggcgcg tgccagcctg
     6061 gagaacgcgg tgatggacgg gcccgaacgc gccctcaccg gccccatctc acgcggagac
     6121 gtagaaaccg ttcaggccca cctcgctatc ctgccgccgg acgtccggga cttgtaccgg
     6181 atttgcggtc tcgccaccat cgaggtggcc aggcggcagg gcctctccgg agaggccgcc
     6241 gtggcacttg agcgcgtgct gcggcagaat taattcagat ggccgaaaaa ccgataaaga
     6301 tccgcgtacc cgaattgctg gagatgaaga agcgcggcga aaagatcgcc atgctgaccg
     6361 cctacgatgc caccatggcc cgcatactgg atcgtgccgg agtggatgtg ctgctggtgg
     6421 gcgacacgct cggcatggtc atcatgggag aaaataccac gctgccggtg acgctcgacg
     6481 tcatggttca ccatacacgg gccgtggcga acggcgccag gcgcgcgctg gtagtggcgg
     6541 acatgccgtt cctctcctat caacttggtg aagtggaagc ggtccggaac gcgggaagac
     6601 tcatacagga gggccgtgcc tcggcggtga agctggaagg agggctggtg gtggcgccgg
     6661 ttgtacagcg cctggtggag acgggaattc cggtaatggg acatatcggc ctgattccgc
     6721 aatccgttca tatgctcggc ggattcaaac cggtgggcgc ccgtccggcg gaggcggagc
     6781 acctgctgga ggaagccgcc gcgcttcagg aagcaggcgc cttctccgtc gtgctcgaat
     6841 ggatgccgcc ggaggtggcg cgcaagatca ctcaatccct ggagattcca acgatcggca
     6901 tcggcgccgg gccgcactgc gatggacagg tcctggtgaa ctatgacgcg ttcgggttgt
     6961 tcgaggagtt tacgccgccg tttgtgaagc gatacgccga gcttggcgcc gaaatgagca
     7021 aagccgcgga acagtacatc gcggatgtcc gcgagggccg gttccccact gaagagcaca
     7081 cgtaccgggc agcggcggct aaagcaagac cgaaatgaag cccaggatct tgaagggcat
     7141 ccccgaaacc cgcacagcgc tcgccggcgc cgccgggtta gggcttgtgc cgacaatggg
     7201 cgcgctgcat gacggtcacg gaagccttat cgaagtggca agacgcgtat cggccgtcgt
     7261 gataaccagc atttttgtga atccaaccca gttcaaccag gcaagcgact atgcgcaata
     7321 cccgcgcgat ctggccgccg acgtggagtt ctgtgccgcc cggggcgtgg atttcgtatt
     7381 cgtgcccgaa gcagcggaga tgtatcccga gccgccgttg accacggtgg aggtggcgca
     7441 aattggagac gggctgtgcg ggcgttaccg gccggggcac tttcggggag ttgccacggt
     7501 cgtcgcaaag ctgttccata ttattcaacc ggagcgcgcc tattttgggg agaaggatgc
     7561 ccagcaactg gcgctcatac ggcgcatgac cgccgatctg aacatgccgg tggaaatcgt
     7621 gccggttccc accgtgcgcg acgccgacgg ccttgcactc agctcgagga accggcgcct
     7681 gaattcccgg gaacgcgcgg ctgccccggt tctataccag gccttgtcgg cggccgcggc
     7741 acgggtggcc gcgggtgaga ccgatcccgg caaggtgaaa gccgttgcgc tggagaccct
     7801 ggggcgcgaa ccgctggtgc gtgtagaata cctcgaactc gtcgacccgg agcgaatgca
     7861 agccgtggat cggatcgacg ggccggtgcg cgccgcggcg gcgatatggc tcggtgaaac
     7921 gcgcctgatc gacaacgtgc tttcgcagcc tgtccaaata ccgcgctaaa aacgggcgca
     7981 ggcgcggccc acttcctata atggagtttg gaccttgaaa aggcattgct gcgaaaagtg
     8041 ggcgaggcga tcggccggtt ccggatgatc cgggacggcg atcgtatcgc ggtggcgctg
     8101 tcgggaggca aagattccct cacccttctt catgcgctcc tgctgctcga aaaacgcgcg
     8161 ccggtagatt tttccgtctg tgcatttacg gtggagcaag gcaaattcct gcggcctgtc
     8221 gaaccgctgg gagattatct gcggagccgg ggtgtcgcct ggacgtatta tcacgacaag
     8281 ccatcctttc gcctattgga cgagcagccc gaccatggat gcgacctgtg cagccgctac
     8341 cgccgccggg cggtctacga gatcgcgcgg ggactggggg cgaatgtcat cgctttcggt
     8401 catacggccg acgatttctg cgaagccttt cttcgcaaca ccctgtttac cgggcggctc
     8461 agcgcgcttc cgcccgtggc gcactcgcgc gacaaggagt tccgcttaat ccggccgctc
     8521 ctgttcgtga cggaagattt gacgaaagcg tgcgccgaat ccttgggtgc gcccgtcgtt
     8581 ccttgcgggt gttctcagaa aacgggaacc gtacgccgct ctcttcgcga tttttttggc
     8641 gaactggagc gcgactaccc gaacctgaaa gaaacaatgc tcgcggccat gggcaacatc
     8701 gagttgcacc ggctgctgga tccccgtttc atcgaagccg aaccgggggc taaggaaaca
     8761 cggcaggaac tgttcccgat tgtaatggaa tcgtaacagc attcgtgttg accctcgtga
     8821 aaagcggatg agatgatggt ggtcttcaaa ggatctactg tgaacgttga ggaattatga
     8881 gattcctgcc ccgcgaagaa aaattcttcg ctcttctgtt gaatcaagcg aacatcattt
     8941 tggaagcggc atcggcgttg cttgcgggcg ctcaagccgg taatgcgcat ttagccgagt
     9001 gcgccgcccg gattgttaaa ctggaacagc aaggcgatga agtaatccac gatctttttc
     9061 tccgcctgaa ccaaaccttt attacgcctc tcgatccgga agacattcac tctctggctt
     9121 cccatctgga tgacgttctg gacggtattg aagacgccat ccatcgtatg gtggcttacc
     9181 gggttgagcc cattccgccg gaggtcgttg aactgtgcgg aatcgttcat gcctgtgctc
     9241 agtccttgca gagcgctttt gaagctctgg agcgcgacga gaaacttttg cagcactgca
     9301 tcgaaatcaa ccgccaggaa gagaaggcag accacctggt gcggcgtgcg gtagaggagc
     9361 ttttcgaaca cgagaaggat cccatcaagc ttattaagct gaaagagatc tacgaatttc
     9421 tggaggacac cacggaccgc tgcgaggatg tggccgatgt cctgcagaat gtcgtcgtaa
     9481 agaacagcta gtaccgtcga tgaccgctac cctcgtccta gtgatagcga tcgttgtcat
     9541 cgcactgatt ttcgattact tcaacgggta taacgacgca gcgaactcca ttgccacgat
     9601 cgtggcaacc cgcgtactga cgccttttca ggcggtggca tgggccgctt tcttcaactt
     9661 cgtctcggct ttcagcttcg gcaccggcgt ggcgaaaacg gtcggcgcgg gcatggtcga
     9721 cctcgattcc gtgacgcctt atgtcattat ggcgggtctg atcggcgcca cgctatggct
     9781 cgcgaccact tcctttctgg ggctgcccgt cagcgcctcc cacgccttgt ttggcggtta
     9841 tgccggcgcc gcgatcgcgc gcgccgccta tgtcaagggc gtttcgaaca tgttcgatgt
     9901 catcgtctgg tcgggttgga tcaagacgat cgtcttcatg gtggtggcgc ccctgctcgg
     9961 attagtcctc gcctatctgc tgatgaccct gatctattgg ctcttccaga actccacacc
    10021 gaagacgatg gacaattatt tccggaagct ccaattggtt tccgccgcca ttttcagcta
    10081 ttcgcacggc accaacgacg cgcaaaagac gatgggcatc atcaccagcg cgctggtcac
    10141 cggaggatac ctcgcaactt tcgaggtgcc catctggacc atcttcgcgg cgcattcggc
    10201 aatcgcgctg ggcacgctga tgggcgggtg gaaagtggtt cacacggtgg gagcgcgcgt
    10261 cacgcggctc aaaccgcgcg gcggattcag cgcggagacc gccggcgcca tatccgtact
    10321 gttagccacg tatctgcgga tcggggtttc cacgacacac atcataacgg gcgccattgc
    10381 cggtgttggt tctttccgca ggctcaaggc cgtgcgctgg ggcgtagcga aaaaaattct
    10441 ttgggcgtgg atattgacga ttcccgcttc cgccgccgtg ggctggctct gcttccacct
    10501 gtttcacatg ttcgttccaa acgcctgatt ccgcgggcag cgcacttcga gccttaacct
    10561 cccgcgatca gccccggtga gtgcgacaac gatgcaatca ggcgctcgcg gttgcgttga
    10621 agcgcgccca cgccggactc gagcgcgata cggtagacga tacccgcgaa aaccgcaatc
    10681 agaaacaaca ccacccagaa cgccgtttcg ctcgcgaagg caaaccgggc agcgaaggcg
    10741 agcgaaaccg gaacaaacag cagcgggtaa aaaaccagca gcaacgcgcc acggcttccg
    10801 gaagaagacc gccaactgtg ctcgggattc attggccgcg catgcctggt tgcgctgata
    10861 ttcccgtcgg ccatcagaaa aacggaatac accagggtga ccgcgtatgc ttccgcaata
    10921 gtggaggctg atgcgggaaa ccgcagtgcc atgcacacaa ctaccacggc cgtcgcttcg
    10981 agcaggacaa tgacggccgc cacaatgttt ttggccagca gaacggctcg aggccgcacc
    11041 ggcgtaacgt aatacaactg cacggccgca cgatcgaacc ccaacgaatt ccagaataga
    11101 acctcgccta ccagcagcag ggcgtagaga cacacgaggg tcagataatg ttgcgtcaga
    11161 aaagagtcct gcgcgtgcct tccgccgaag agcattggaa tccagatcaa cagcccgaaa
    11221 gaaaagccca tgacaaacac cagccggaac cgcggggcgc gggcgagcgt gcgcagttct
    11281 ttctcgacca aagcaccggt ggggtcgcga aggaagtgcg aacacaggtc gaaaacagtg
    11341 cgggataagg catccatccg cgacgcatga actcttcgcg cggccgttcc cgcttgcgat
    11401 ctcaaccctc cctcaaactg tcgccggccg aagaagaaag cagccacaat ccatcccagc
    11461 aggctgagcc aggcgggcac cgacacctca cccgccgcca gccgccccgc ggcggcccac
    11521 ggcagcgcca cggagtgcgt ttcctcaaat tgccgtctca gcggttccgg cacccctttc
    11581 cataggatgt attggggtag agcgactacc aacatcatca cgagcaccac cagctcacgg
    11641 atgcgccgct tcgcggtgaa gcgcactaac gcgcttttca ggccagcgga caggaacagg
    11701 ttgaatgcca cgtaaagcgc cagcgccagc gaggtccacc acgctatacg ggggttgagg
    11761 aaaagcccca ccgcggctcc tgcgatcagc aacagcacct caaagccggt gagagcgcgc
    11821 aataccagtt ccaggaggaa aagctcgcgg cgcggaatag ggtaaaccag caaccgcgaa
    11881 atatccagcg aggctccggt gctggccatc agcaaaggcg cggcctgcca gtataccgta
    11941 gcgagcagca gaccgtttga aagcacgacc gccagatcaa aagcgggaga aggagcggac
    12001 aacaggatcg cgatactagc ggcggcaaga gcccacattc cgtaccagag gaccgcgacg
    12061 gcggcaacga gatacatgcg gacgccgcgg cctcgggaca cgctgttgcg aagcacacgc
    12121 cattgggccc ataagatggc ccgcgcatag gaaggcatcg cgttacaacc agtccagggg
    12181 ttccgaggct ctcgttcccc ccaccagcct tacgaagaga tcttccagtg tttccgagcc
    12241 tgtccgcaga tcctgcatcg ttccctccgt gaggagcctt ccatgatgaa taatcccgat
    12301 gcggtcgcag agtctctcga ccacctccag cacgtgcgaa gtcagaaaaa tcgtcgtgcc
    12361 tctcctcacc tgctcgatga gcaactcttt catgaaacgg gcgcccacgg cgtcgacccc
    12421 ttcgaacggc tcgtccaaca gcagcaattc cggcttatgg atgagcgcgc ccgccatcgc
    12481 cacgcgcttt ctcatccctt tcgaatactc cgcgatgagt ttacgccggc tctcgcccag
    12541 atcgaacatt tccatcagct cggacgcgcg ctcgcgggcg actttacgat ccagcccgta
    12601 aagccgtccc accagctcca gaaattctcc accggtcaga tgatcgaaaa gaagcgactc
    12661 atcgggaacc agccccatct tcctcttgat ctccatctcc tgggtgggca tgctcatccc
    12721 gagcagttcg accgacccgg cgtccggctg cgccaggccg gtcagcatgc gtatcgttgt
    12781 cgtcttcccc gctccattcg gcccgagaaa tccgaagaag gagccacggg gaacggtcag
    12841 cgtcaatcca tcgacggcgg tctggccgcc atacgtcttc cggagatcgc ggatatcgat
    12901 ggcagctccg ttcatcggac cgcttaggac ggcagcttga cggctggaat gcgggactgc
    12961 acagggggca acgaaggaaa gtccgtgtat ggcgtgtcct gataccagta gcccacggaa
    13021 aaccagttgt cgccgcggtc gttcgcgtgg ccgtgttcga gggtgtgctt cagataccgc
    13081 gtgaacgtca ccggattgtc gccatgccag cgatagcagc agtaacggcc tccggtgcgc
    13141 tccggcatga cgatgatggg cgcgccgtaa tatttgtgcg cgaaggggat ggcgccgtcg
    13201 cggcctccga agttccagct tccgaggaag taatcctccg atccggttcc gacgatcgcg
    13261 ggctttgtat cgttatcgac gaagatcatg tcgtctcctt cgcccatcca accgtcggcg
    13321 ttcagcaaca cgcccagcgt cgctcccatc aggtgtccgc ggccctttgt ctcgatgatg
    13381 acgtggtttt gcgcgcccaa cgggttcagc ttgtctccct tgttcgcgac gcagggtgtg
    13441 cattgcctgt actgcgcgtg gaaatagaca atgtcgtccg gcagcgaagc tacacgcatg
    13501 aagtcgatat tcgagtagaa ggaattcacc ggatccgcac cctggttctc cacggtcata
    13561 cgcgctctgc ggcggtacgg catggagaag tagcagttca aggatcgcag cggcgaacac
    13621 gccaggtagg cggactcgta gatctcatac tgtcccaggt tcagtccgaa gaaatccccg
    13681 atgggcgctt ccacactggg cttggagcta tcgtcccaat aagcgcgcag gaccaactcc
    13741 ttcaggtgat tccgtccggc agccgcaatg gtgaaccaga tgtgcgagat cacgccgggt
    13801 ccggcctgat tataaatttc gagcgtttcc ccaggcttga tcggccagcg gtccgagttg
    13861 ccgccggtct gatcgaaact ggattgtttc catgactgat agtgaagcgc acgggcatac
    13921 cgcgggagat aatcaaactg cggcgcgcgg gcaggttgaa tctgagcaag ggaagccgct
    13981 ggcgcggcgg cggaaaagcc acctaaagta agaagtcttc gcaggaagcc gcggcgcgcc
    14041 gcgggcggag atgaaatgtg cattgcgtat cctcgtccct gcaaatatac ctgaacgagg
    14101 tccgctgttt aaaggcgtgc tacgccgtcg ccgcgttgcc ggccgcgagc ggcacgttct
    14161 ctttcaccag ctcggcgttg atcaattcgg agatcacggc ggacagatgg cgattgcgca
    14221 gcgcccatgc gtaagctttc tgcggcgctt tcaacggacc cggaaacgcc ttttcgatca
    14281 tcgccagccg ccgggggtct gcctgctggc catcgcgccg cgcgctccaa acaggccggg
    14341 ccaccttcat ggcgatcacg cccaaccccg cgtcatgcgc ggctttgagt gcagtgtcca
    14401 cgaaacgatg attgaccacg ctgtaggcga ccatggccac ggagtagtgt ttctgctcca
    14461 ccgccgcccg gagaatgccc gcaggatcac tgtgcgccga cacgccgaga tgccggacct
    14521 tgccttgttt cttgaggatt tcgaaagctt ccaggatctc cggatagttg cgcaactcgg
    14581 cgggagtgga ggcgccgtgc ggacacatca gaatgtcgac atggtccgtt cccaaccggc
    14641 ctaagctggc gtggaccgaa tccaggacga tctgcttata gtttttgccc cggtcgagct
    14701 tttcgccgta cttctccgcg atggcgttgc tcaacgccgc agcctccatc tcgccgcgct
    14761 ggccacggaa gtaatcgccg acgtaatcgg gttcgaagac ccgtgtcttt tcgatgtatt
    14821 cctgtgccgc atggcggact ttcgtctgct ccgtctctgg caggctgtcg aagatgtcct
    14881 tatacagctt gttccggttc aggtcccacg ggctgacttt cgtattgaga aaaaaactgt
    14941 cgcgcgggcg cgacttcaac acctgcgcgt agccttcctc gctttttccg gagccgtagg
    15001 cgggcgcggt gtccaggtag ttgagcccca tgtccatcgc cagcagaaca tgctcgtaat
    15061 tggtgggggc gatggtgttc ccacccatca ccattcgcga gatcatgaac ccggtccggc
    15121 ccagtttccg ataggccatt ccggattgcc ggttccgcca ttcctcgccg ggcgccgcgg
    15181 caatcgcagg cagcacgccc acggcacagg atgccgccgc ggacacaata aatcgtcgtc
    15241 gatcaagcat tggagtgcct cccaggggca gcgaccatcc tatcaactcc cgatacccgc
    15301 ggggatcttc acgggcggct cacgtccttc gagctcgagc ttcgtcgata tgaaacgaag
    15361 caggtccttc agcgacacga tcgcgaccaa ctctccgttt tccaccacca tcatgcggct
    15421 gttccccgtg cggctcataa gagacagcgc ttccatggcg tccgtatccg gaccaatgac
    15481 gttcgattcc gagcagggcg aggatatttc ctgaaccgtg tggttgtccc actcttcgcg
    15541 ggatacattc ctgacatccc gcgacgtcac gcagcctagc agccggttgg attcgctcac
    15601 caccggaaac attttgtggt gataacggta gatgtactca tccaccagtt cgcgaatgga
    15661 tatgctccgt ggcaccgtta tcgggtccgt cttcatgaag cgccgcacgg attctccttc
    15721 gagcgttctt ttaagcacca cctgctgata cgatgcctgg gaagcgccgc gaacgaacat
    15781 gccgatcaaa aaccaccaca tcgccgtaat gaagttgccg ctgaagagcg tgacaacaga
    15841 gagcatgatg agcagcaggc caaaaccgga accgaacgcg gcggcgatgc gggtggcgcg
    15901 catcaaatcg cctttccaat gccagagcgc ggccctcaga atgcgcccgc catccagggg
    15961 aaaagccgga atcaggttga acgccgccag aatccagttc atccaggaca ggtaggcgac
    16021 gaccccgacc gcgaacgtac tccaggcgtc ccgcccggca acatacgcca gatagaagag
    16081 aaacccgatg acgatgctag cagccggtcc ggcagcggcc atcatgagtt ccgcccgggc
    16141 attctgtggt tcctgctcca tctccgccac accgccgaag atgaagagcg tgattccttt
    16201 catctgtaag ccgtagcggc gggccaccag ggaatgagac aactcatgaa acacgatgga
    16261 cgcgaagagc ccaatcgcgc caatgatcgc catgagccag taatcacggc ctgtcagtcc
    16321 cggaaattgc gcgggaaaga atccgacggc aagcgaccaa gccaccagaa tcgcaatgat
    16381 gaaccaactc gcgtcgatac gaaccgcaaa cccgaacagc ttgaacagcg tgatgcgccg
    16441 gccgaacatg aaagacctcc tcgaaagatt ggatggcagg atgattgctt tagtgtcaga
    16501 aaagggagga gtcaggagcc ggaactatcg aagcccagcg cttcgagata gccatggttc
    16561 agttcgcccg cctccaggcg gatcttgtcg agaaggtccg tcaaggcttc gtgaacctgc
    16621 tcccgggaag gacgaagcgg cgctttcttc tccgcggcgg cgacgccgcc aggcaaccgc
    16681 gcataagcga cgatagcatc ccaatgccgc ggcttcggga aactgacggg tgcgctggtg
    16741 gtctgcatct tcttccacgg ccaaaaagtc caatggatgc catcggcgtc cagggtttgg
    16801 cgaaattcgg agatccactc gttcgtgttt tcgccggact cacccatcag caacggaacg
    16861 ttgtacttct tcgcgtagcg gagatattct tcgatcactt cgcgggacgg cgcggtccag
    16921 tatttatgga aggagtacat ggactgggcg tcgaacggcg gcccgaacga atcgaagcgg
    16981 ctggcccact gcgtcccctc caggatcacg atatggtgag gatctacttc gcgaatggcc
    17041 gcgacgatgc gtttgtaaag aggctccagc ttcggattca gcgcaggcag gttcccgaag
    17101 tgagggatgg gttcgttcag aaggtcgtag cccagcacca ccggttcgtc ccggtagcgc
    17161 tccgcaatgc ggcgccagag ctggatggtc gcctcctggg cggtttcgct ctcaaaaagc
    17221 cacgggtaac cccaactgtc gtcgatattc gtgcccgtct gcccgccttg ggcggcatgc
    17281 agatccagga tgacccagag attgtgcttc ccggaccagg cgatgacatc gtccagacac
    17341 ttccaaccgt ctccatccgg ctccagcaat ttccagtgca tcggcacacg gaccgtgtta
    17401 aagccgatcg aagagatctt cgcaaggtcc gcttccgtga tgtaccggtt gcgccactcg
    17461 cgccagaagc gggcggtaaa gtcctcgccc gccaagtccc ggaagagagc atccatttcc
    17521 caccaggact cgggagcccc ttcgtttaac agcagcatgt agccttccgg cgccagccag
    17581 ttgccaaggt tgatgccttt caggagaacc ggtttgccat ccggcgcgac gaggcggccc
    17641 tcccgtacct ggacgaaagg cggcgcggcg gacaatgcgg cacacaaaca gagagcggat
    17701 aacagtcgca tcggtacggt ttcgagtgaa atcgggtccg tggccggacg agaccacggg
    17761 cccggggcat atcgccctca gaaataaacc ttcagcccca gctggacgac gcgcggcccg
    17821 ccgcctccgg tatacgtgtt cgtgtactcg ccgaagtact gtccgtagtc gcccgcctgt
    17881 cccacacccg ccgcgcgcag cgggtttgtc gtgagaaccg gaccgctgga gccgctcgga
    17941 ttgctgatcg acataccgag attcacgtcg cggaagttcg tctggttgaa tacgttgaag
    18001 gtctccaggc ggagctgaat catacggctt tcactcgccc ccagtttgaa attcttgaag
    18061 aggctgacgt cccagttctg cagtccgcca ctgcgcatgt acgtgcgggg ccagggcgcg
    18121 ggaacgccga tgcccggagc gcgaaacgcc gccgggttaa acttcgaagt gcccgtccgc
    18181 tggttcacgt cgccgtccac gtaaacgaac aacggcggtg attcccagtc cgtaatactt
    18241 cccgtgatct gcttcggatc gatccagagc gccggctcca ccggtgcacc ggaagaaaag
    18301 cgggtgatac cggagagctg atatccgtcc gtcaaatacg aaagccactt cggcccgccg
    18361 aaggccctgc tgaattgtgg caggtcatag acgtagttga acgcaagcac gtgcggccgg
    18421 tcccaggacg ccagccggta gtcgtagccg cgcgtgtcga aggggttcgt ccagttctcg
    18481 tcgccgttcg ccgtgaccag cgacttcgac caggtgtagg ccactccgaa cgtcagtcct
    18541 ctggcaaagc ggcggtttac ggccacctgc aacgaattgt agttgctgct gccgtcaaag
    18601 gtgtaatgtt tgatctgcgc atagccgggg tacggacgca ggtagttggc atccagcgcg
    18661 taagcacccg agaaggcata gcctgcccta ctgtacgctt cgggaaggtt cggttccacg
    18721 gcggggacga caccgcccgg aaacttcgcc ggatcctgcg cccatcgctc gaatgctgtg
    18781 ccgtaaggaa tcatgttgag gttgcgcgtc tgcaccaggt gccgcgagag cgtaccgacg
    18841 taactcacgt ccatcgtggt gctccatcca agctcccgct gaatgccgag cgagaagctg
    18901 tagatcgtgg ggacgaagcc gtcccgggcc gcgccgaata atgtcggcgg cgccaggatc
    18961 cccgagccgc ccaaagaaga cagattctgt acccgcccgt cgaccaagcg cggattgcgc
    19021 acgttcggcg ggttttcaaa cacagggttg tagccgaggt tcccctgtgt ccggtcatgc
    19081 atgatgcccc cgccgccgcg gagcacagtc ttgccgtcac ccgtcatctg ccaggcgaag
    19141 ccgaagcgcg gctcccacat gattccccgc gagtcgaagc cgccgcgggg gtagccggcc
    19201 gacgccaggc cgattccatt tgtgagtgag ccggaatctg gaaccagagt cccggtcaga
    19261 tagccgggca gggtcgcgcc cggattggcc gggtcgtagg cgagtccgtc ggccgtagct
    19321 cgatagatgc gcggcgccgc cgaggcatcg aagagatagg gattgaagac cgcgccctga
    19381 tcgttcttgt cgtattgagg ctggtaccag gtcattcgca agccgtagtc cagcgtgagc
    19441 cgcgacgtca gccggaagct gtcttgcgcg taccattcca cgttcgtata gcggaaaaac
    19501 ccgcggggcc ggttgctgct ctgcacgaaa tcgcggtagt gccctgtcag agcggcggcg
    19561 tacggatggc ccgcgagctc gtcgttgaag ttaaactggc cgttgacgtt gccccaggcg
    19621 ggctggtcct tgcggctgcg ctgcacaaaa atgccgaact tgaaaatatg ccggccggcc
    19681 actttcgtga ggttatcgga aaaatccagc gtggtgttcg aattgtagaa cggtaccgcg
    19741 cccagatagc tccaggtgaa actggtgttc ggtgtacagc aaaagctgaa gtcgggaatc
    19801 ggagtggcgg cgtctaccgg gaacagcaac ggaaggtcaa ttccattggc gccgcgggag
    19861 atgttaccgt ccctgccgtc cgcccggagc ttggagacgg acgggccgaa ggtgaattcg
    19921 ttcaccagcg aaggggagag cgccgacgta aggttcagcg aagcgttcca gcccggctcc
    19981 tcaatccgga tgccgccggg aaaagcgatg ttcgagatgc cccacaaaaa ggagccatag
    20041 ggttcgttgc ggctgctgtc gttattgatc cagcggccgt agaggcggtg catcgccgaa
    20101 agctgatagt cgacgcgcac aatgtcttcg cggcgcggat actcggtgga tgcctgcgtc
    20161 gtgaagttgt agtcgtatct tccgctgaga ttcggctgcg ggtaaagccc gagagcctgc
    20221 cgcatgcccg gcagaatcgc gctttcggga ataatattgc cgggatacgg ctggccggtg
    20281 gtgggatctc ttacaacgac gggcgcgcca aaaccgtcaa gggtctggga gaaatcgccg
    20341 cccaactctg ccggtgtcgg aacgcgaacg ttggtgatgc cgccggggat cagttgccgg
    20401 taatactcct gtccaaagaa gaagaacagc ttgtctttgt tccggttgaa attggttccc
    20461 ggcaggagaa ccgggccgga aacctgatag cccgcgtagt tatagcggta cagcgcgcgc
    20521 ggtgaaacct cgcttccatc cggctgccgg ccacgcatat tgttaaagaa attgttcgcg
    20581 ttcagcccct cgtgacggtg aaaccagcgc gcattggcat ggaactcgtt cgagccgcct
    20641 ttggtcgtaa tcaccatcgt gccgccgccg gctttgccgt actcggcctg gtagttcgag
    20701 gtgagtattt tcacttcggc gatggcatcg ggattcaacg tgacgtgtga agcgccgttc
    20761 gaacccgtat cgacattcga ggagccgtcg atggtcaact gcttttgatt ggaacgggtg
    20821 ccattgatgt tgaagctgtc caatccgccg cggttggact gttcaccatt aaattcgctg
    20881 gtcactcccg gtatgatctt caccatgtcg aaaatattgc ggccgttcat ggcaagattg
    20941 ttgatctgat tgttggtgac aagcatcgag cgctcgccgg attccgattg caattgcagt
    21001 tcggcggcac cggcttccac cgtaacggtc gttgctgtag ctcctaactg g
//
LOCUS       DASTMK010000330        13484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_496626, whole genome shotgun sequence.
ACCESSION   DASTMK010000330 DASTMK010000000
VERSION     DASTMK010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496626"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VFQ79_14355"
     CDS             complement(<1..941)
                     /locus_tag="VFQ79_14355"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEU0140901.1"
                     /translation="MNTIDGLPLSSLEKQYREDLFEDFLPFMDKFVVDHEVGGFLCAA
                     DRDGTRLNSDKDNWFDGRGIWVYSFLYRNFTHDPRHLEIARKTVEFVLSIRPKDGVLW
                     PRRYTRHGEPLAPDGEIYGTVFMAEGLAEYAAASGEERYRDLAKEILLQCAEVYERPD
                     YALNPSHYFGRDVPLLPGGRVQGAPMVLLRISTQLLRARADKDLEKIAAWAIDAIMTR
                     HYNPSVALNYEFLNHDYTVPANEYSQFCYTGHTLEIMWMVMDEALRRGDKALFDTAAE
                     RFRRNVDVSWDDVYGGVYRGMSHLDRNEWFLQKVLWP"
     gene            complement(941..2743)
                     /locus_tag="VFQ79_14360"
     CDS             complement(941..2743)
                     /locus_tag="VFQ79_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140902.1"
                     /translation="MMNAFRSMLRILPRLALLPAALALGGSTDSATLAWYMRETAMTA
                     DGRLTFLSKSWWPKAQALAEGQSFTMDLNRDGRPDTIVKRENGHIVQAIDDSGRTTEI
                     WNEVSTAYLVSYKGTGLVDRMIVYIDNDGDGKTDEMEMRHYRDGYLRYAWFGENYDDD
                     GVRIFDIKDWSYVGNNGKSKFRGNLQIYLNKYDPLTKQWLPLSECPFAFWDDNKDGHG
                     DTVFRVSAAPLASLTGPDTDYANNYNYMWAEKATPLAEMGNLNVRLSFNIDPGTRNDP
                     LDKPHYNFGFTMVGSEPYKYPNMTYTNPKRRPPQTVTRIDWKDGIAIGMSYPARETGF
                     TWDESRSVWRWEGQFWLYERIYLSNTGGPTARWNMRREYSPAPVAERRLYYSDADKRY
                     HLFGATEGWLEAGHLVNKKKDLEFRWSDRDGNGYLDTVQVFEGDNAIPSRTSHYKPRA
                     AMMKLDREALAREYNGRILPQAIQENLDLIASLKRIANSPAAEGYEAEAAKAEMLERK
                     RYCLDIARELYFLRARQLLREKSAAGPYPSAPPDRKKWRSTEPGSRDTGYSMGDTLRF
                     WKIERQIAAFETSYAAGRLKEAREHLEQIENELK"
     gene            2889..3347
                     /locus_tag="VFQ79_14365"
     CDS             2889..3347
                     /locus_tag="VFQ79_14365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0140903.1"
                     /translation="MDTRRRSFMKMAGAAGAAGTAGSMALMAQGAKGTRTRARGGIRL
                     GNLFFSSGLTGVRPEAQKDPLAFGGDIKEQTERTLQAHKANLEALGSSLENVLKVTVF
                     MADVKTEKNAMNEAYAKFFPKDAPARSCVGVEFPDSQTRVEIELVAWIPD"
     gene            3548..4330
                     /locus_tag="VFQ79_14370"
     CDS             3548..4330
                     /locus_tag="VFQ79_14370"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DtxR family transcriptional regulator"
                     /protein_id="HEU0140904.1"
                     /translation="MHAKQRSEATTLSASLEDYLEAIFHIASEKQVARAKDISARLGV
                     KNSSVTGALRILSSRGLVNYAPYDLITLTPGGAEAAREVIRRHEALRDFFVKVLNIDP
                     ETADEGACKMEHAIPLPILERFIQFVEYFETCPSAGAQWVRDFGYTCRGGAERENCAQ
                     CVSAEAEERTKGKLKETTMKLSDLKIGQRARMVKLAGHGELRKRLVDMGMVPGAILAV
                     ERVAPLGDPIDIKLRGYHLSLRKSEAAMVTVAAIGAAGQVTA"
     gene            4327..4566
                     /locus_tag="VFQ79_14375"
     CDS             4327..4566
                     /locus_tag="VFQ79_14375"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEU0140905.1"
                     /translation="MTSCHSLPLPLHLAGGGRFVRLHRVTGNSETQSRLAALGLIPGA
                     ELQLVGGSARGPLIVGLKSGRLMLGPELARHLWVE"
     gene            4632..6677
                     /gene="feoB"
                     /locus_tag="VFQ79_14380"
     CDS             4632..6677
                     /gene="feoB"
                     /locus_tag="VFQ79_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEU0140906.1"
                     /translation="MIAVKKITVALAGNPNSGKTTIFNNLTGARQHVGNYPGVTIEKK
                     EGSCSYRGAEIQVVDLPGTYSLTAFSPEEVVARRFLVEEAPDAVVAVLDAANLERNLY
                     LAMQLLELEVPLILDCNMMDLVTGRGDRLDLRRLSAKLGIPVVATVGNQRQGMEELRE
                     AVASSNGVEGRKQLSPRYSEDVEASLAALEQEIQNAAPRSRFPARWTAIKLLENDPAA
                     NEEWRGTSVAEFAEQERAMLEQARGEPAEIVLAAERYALIGTICADCYWPAASPQRSR
                     SDVIDAVVTHRALGLPIFLAAMFAVFWATFNVGAQPSEWIEVGVAWLGGAVSGWWPAG
                     SESSLKSLLVDGIIGGVGNVIVFLPNILILFFAIAMLEDSGYMPRAAFIMDRIMHKIG
                     LHGKSFIPLLLGFGCTVPAIMATRTLESRRDRLTAILVAPLMSCGARLPIYSLILPAF
                     FPQVWQAPLLWGIYVLGIVLAVVMAKLLRLTLFKGEPAPFVMELPPYRIPSLRDGLLH
                     TWERSRLYLKKAGTIIVGISILLWALTNFPKPPAGAETGGSSELAYSYAGRAGHLMEP
                     VLAPMGFDWKIGTALVGAFAAKEVFVAQMGIVYAVGEGEEAEAPLREQLRNTYPPLVG
                     FCIVLFCLIGTPCMTTLAVTRREGGEWRWALLQFGGLTVLAYVVTAMAYQMGKLYFG"
     gene            6696..6890
                     /locus_tag="VFQ79_14385"
     CDS             6696..6890
                     /locus_tag="VFQ79_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEU0140907.1"
                     /translation="METVIVIFVVAAAAVLLLRGLYRNAKTGKPMCGCASAGGGCQMS
                     NSCGQSRVKKVTTHGQPAAS"
     gene            6868..7170
                     /locus_tag="VFQ79_14390"
     CDS             6868..7170
                     /locus_tag="VFQ79_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140908.1"
                     /translation="MDNRQLLEQSWEELQAQMEKHRHLVRSALEGCATGRTECLWCDC
                     PHRRTLLNLAAHSVKVLDDTRKAFKSKQLEALRKDLLRVLTEEVRSGQTANGQPRE"
     gene            7415..8491
                     /locus_tag="VFQ79_14395"
     CDS             7415..8491
                     /locus_tag="VFQ79_14395"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEU0140909.1"
                     /translation="MELPVLLHQLEERGITLSLGYTAEIFSAVSGGVGRGRRSDYLGS
                     FAPSLTLDFSRIGFGRGQFYMSGQTLHGRGISESRIGTAQALSNLEEQRFGRFIEAWY
                     ADSYRDGLLKVKMGRQYADADFGVVENGADFLNSSYGLNPTTPMPTFPAPELGASVWV
                     APASWFSAGAGVFKGGTLDALTEDGAAMNRRPFAMFEARFEPFSKNAASHGQYSIGLW
                     RQSRGAWLSAGEENAAPVRNYGIYATADHWFMRSPSGEHRGPGLFFQWGWTPADRNEI
                     TGYTGGGVAWAGLIPARRRDSIGMGVTRARLVGRDSENIIEWFYKLQVSSKLMVQPDL
                     QWVLNPCGDHRRALVAGLRLGVEF"
     gene            8563..9615
                     /locus_tag="VFQ79_14400"
     CDS             8563..9615
                     /locus_tag="VFQ79_14400"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEU0140910.1"
                     /translation="MFRFILLVLAISLTASAQDPLLTKAQRIFKPVPASPPALRANPI
                     TPEKIELGRMLYFDPRLSSSWLISCNTCHNVGLGGVDLLETSVGHGWQKGPRNSPTVF
                     NAVFNIAQFWDGRARDLKEQAQGPVQAAVEMNSNPGRTVKTLSSIPEYVSRFEKAFPG
                     EAAPVSFENMAKAIEAFEATLTTPGAPFDRYLKGDAKAINEEEKKGLQVFMAKGCTAC
                     HAGVNLGGQGYFPFGVVEKPGSEILPPQDKGRFAVTRTASDEYVFKSPSLRNIALTAP
                     YFHSGKVWDLKQAVAIMGTAQLGAELNQAETEAITRFLHTLTGEQPKIEYPILPPHTA
                     STPLPEVGVGPRPAGH"
     gene            complement(9616..11268)
                     /locus_tag="VFQ79_14405"
     CDS             complement(9616..11268)
                     /locus_tag="VFQ79_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU0140911.1"
                     /translation="MYLLFLVLLLLQSPDATVPGEVSAPYPTVTALALEWRIEGDANL
                     NSVVKVSYRKSGEARWREGMPLRRVPAGSSSGTRPIFHWDNKHSGSIFDLEPGTSYEV
                     RLILHDPDGGGATRTLSVRTRPVPRAPAGAKVRKANPATIGGARPGEIVVLEAGSYGR
                     FAPEADGEPGRPIVYRSTDGKAVFESISLDGRKHVYLENLAIENPGGTAVSMVRAEDC
                     VVRRCRIRATYGVRASKAPGAKNCYISDNIIDGPTPWTNEAMGASGKNIGEGIQLTGP
                     GNVICFNRVTGFRDCISNMEEENVTEQISIDIYNNDIYVGADDAIEADFCFHNCRVMR
                     NRITNSFVGLSSQPSLGGPTYFLRNVMYNLTYVPFKLHRGSRGDVIAHNTTVKAGDGM
                     ACFSGVPFDHAWFRNNLSIGGPSGGVRWGGYGGGSGMAAQINSHGPNSSFDYDAVGTY
                     QTPFKATIGKLDFFEVEPHGVRVDMSVFDGVEFPVSPLTLMTPPDLRPRPGSPVVDAG
                     LRIPGINDGFLGKAPDIGAYEAGQPLPWYGPRPDGIDEATTR"
     gene            11379..12458
                     /gene="ada"
                     /locus_tag="VFQ79_14410"
     CDS             11379..12458
                     /gene="ada"
                     /locus_tag="VFQ79_14410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEU0140912.1"
                     /translation="MNDSTKNARRRDATVNDARWASVLARDAGADGQFFYSVDTTGVY
                     CRPSCAARRARPENVSFHLTSEDAKKAGFRPCKRCRPDQLPVAGPHAAKIAAVCRIIE
                     NSEVMPTLEQLSRQAGLSPYHFHRVFKAMTGLTPKKYAAAHLEKRVRRALGSSATVTE
                     AIYDAGYNSNGRFYETSNEVLGMTPSSYRAGGARTQIRFAVAECSLGSILVAQSKRGI
                     CAILLGDDPEVLTRHLQDQFPRATFTGGDAGFEELVSKVVGLIETPALGLDLPLDIRG
                     TAFQQRVWQALRKIPVGSTASYAEIARKIGSPRSVRAVAQACGANLHAVAIPCHRVVR
                     TDGALSGYRWGVERKRALLEREART"
     gene            complement(12477..12944)
                     /locus_tag="VFQ79_14415"
     CDS             complement(12477..12944)
                     /locus_tag="VFQ79_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0140913.1"
                     /translation="MDRRQLLKRLDKAWQEFRQSYAGLSDSQMLEPGVTGGWSVKDIL
                     AHVTTWEEEALKNLPLILKSERPPPYSATYGGIHAFNARMTEQKRDASLSDVLRQLEE
                     THTRLLAYIDSAPESQFTNETRFRRRLRLDTYSHYPKHTEAIRKWRERGRAAL"
     gene            12988..>13484
                     /locus_tag="VFQ79_14420"
     CDS             12988..>13484
                     /locus_tag="VFQ79_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU0140914.1"
                     /translation="MKNIGERLEAVDWERVWRDLDEQGSAVIGSLLSPGECRLIAGLY
                     SADGPFRSHVVMARHGFGRGEYKYFKYPLPALVAELRTAIYPHLVPVANRWNEVMNTG
                     VPYPERHADFLARCHQAGQVKPTPLLLQYEAGDYNCLHQDLYGVHVFPLQTAILLSEP
                     GKDFTG"
BASE COUNT         2613 a         3909 c         4183 g         2779 t
ORIGIN      
        1 tgcggccaca atactttttg cagaaaccac tcgttgcggt ccaggtgaga catcccgcga
       61 tacacgccgc cgtagacatc gtcccaggag acatcgacgt tgcgccggaa ccgctctgcc
      121 gcggtatcga ataacgcctt atcgccgcgc cgcagcgcct cgtccattac catccacatg
      181 atttcgagcg tatggccggt gtagcagaac tgcgagtatt cgttcgcggg aaccgtgtag
      241 tcgtgattca gaaattcgta gttcagcgcg acagaaggat tgtagtgccg ggtcatgatg
      301 gcatcgatgg cccaggcggc gattttttcc aggtccttgt cagcgcgcgc acgcagcagt
      361 tgtgtcgaga tgcgcagaag caccatgggg gcgccctgta cccgcccgcc gggcagcagc
      421 gggacatctc tcccgaaata gtggctcggg ttcaaggcat agtcggggcg ttcgtagact
      481 tcggcgcact gtaaaaggat ctccttggcg agatcccgat accgttcttc tccgctggcc
      541 gcggcatatt cggccaatcc ctcggccatg aacaccgtgc cgtagatttc cccgtcgggc
      601 gccagtggct ccccgtgcct cgtgtacctg cgcggccata gaacgccgtc cttgggccgg
      661 atgctcaaga caaattccac ggtcttgcgg gcgatctcca ggtgccgtgg gtcgtgtgta
      721 aagttgcggt agaggaacga gtacacccag attccacggc cgtcgaacca gttatcttta
      781 tcgctgttca ggcgtgtgcc atcgcgatcg gcggcgcaca ggaagcctcc cacttcgtgg
      841 tccacgacaa acttatccat gaaggggagg aagtcttcga acaggtcttc ccgatattgc
      901 ttctccagcg aggagagcgg caatccatca attgtgttca ttatttgagt tcgttctcaa
      961 tctgctcaag gtgttctcgg gcttctttca gccggccggc ggcataactc gtttcgaacg
     1021 ccgcaatctg ccgttcgatt ttccagaagc gcaacgtgtc gcccatggaa tacccagtat
     1081 cgcgcgaacc gggttcggtg cttcgccact tcttgcgatc gggcggcgcg gatggatagg
     1141 ggcccgcggc gctcttttcg cgcaacaact gccttgcgcg aaggaaatag agttctctcg
     1201 cgatgtcgag gcagtagcgt ttgcgctcca gcatttcggc cttggccgcc tcggcttcgt
     1261 agccctccgc ggcgggtgaa ttcgcgatac gtttcaggga ggcgatcaga tccagattct
     1321 cttgaatggc ttgcggaaga atccttccgt tgtattcccg cgcaagcgcc tcccgatcga
     1381 gtttcatcat tgccgcacgc ggtttgtaat gactggttcg cgaaggaatc gcgttgtcgc
     1441 cttcgaagac ttgcacggta tcgaggtatc cgttgccgtc ccggtccgac cagcggaact
     1501 cgagatcctt cttcttgttg acaaggtgtc ccgcttcgag ccagccttcg gttgcgccga
     1561 agaggtgata acgtttgtcc gcgtccgaat agtacagccg ccgttccgcc acgggcgccg
     1621 gcgaatattc gcggcgcata ttccagcggg ccgtcggtcc gcctgtattc gacaggtaga
     1681 ttcgctcgta aagccagaac tgcccctccc accgccagac cgagcgcgat tcatcccacg
     1741 tgaacccggt ttcccgcgca ggataagaca tgccgatggc gatgccgtct ttccagtcga
     1801 ttcgggtcac ggtctgaggc ggccgccgct tcgggttcgt gtaagtcatg ttcggatact
     1861 tataaggttc cgaacccacc atggtgaacc cgaagttgta gtgcggtttg tcgagtgggt
     1921 cgttccgcgt tccgggatcg atattgaagc tcagccgcac gttcaggttg cccatctccg
     1981 cgagcggcgt cgccttttcg gcccacatgt agttgtagtt gttggcgtag tccgtgtccg
     2041 gaccggtaag gctcgccagc ggcgcggcgc tgactcgaaa gactgtgtcg ccgtgcccgt
     2101 ctttgttgtc gtcccagaag gcgaaggggc actcggacag cggcagccac tgcttcgtca
     2161 gtggatcgta cttgttcaga taaatttgca gattgccgcg gaacttgctc ttgccgttgt
     2221 tgccgacata ggaccagtct ttgatatcga atatgcggac gccgtcgtcg tcgtagttct
     2281 caccgaacca tgcgtaccgc aggtagccat cgcggtaatg ccgcatctcc atctcgtcgg
     2341 tttttccgtc cccatcgttg tcgatataga caatcatccg gtcgaccaga cccgtcccct
     2401 tatagctgac caggtaagcc gtgctcactt cattccaaat ttccgtggtg cggccgctat
     2461 cgtcgatcgc ctggacaatg tggccgtttt cccgtttcac gatggtgtcg ggccggccgt
     2521 cccggttcaa gtccattgtg aagctctggc cctcggccag ggcctgggcc ttcggccacc
     2581 aggatttcga caggaacgtc aaccgtccgt cggcggtcat ggccgtttcc cgcatatacc
     2641 acgcgagtgt cgcggaatcc gtggaaccgc ccagggccaa cgcggccggc aacagcgcca
     2701 ggcggggcag tattctcagc atcgaccgga atgcattcat catgccagtt tatgatctcc
     2761 cgtcttattc attgtatgcg cccggttgtc actgtcaaac ggtatttgat aagaacaaat
     2821 gccgtttccg gcccggcttg ccgcgcacac agccattttg aaacaatgtg ccgagtaaag
     2881 gagtgaaagt ggatacacgt agacgttcct tcatgaaaat ggccggcgcg gctggagccg
     2941 cgggaacggc gggatcgatg gcactcatgg ctcaaggcgc caagggaaca cgcacgcggg
     3001 cgcgaggcgg cattcgcttg ggaaatctct tcttcagctc gggtctgacc ggtgtgcgcc
     3061 ccgaggcaca gaaagatccg cttgcctttg gcggcgacat caaagagcaa acggagcgca
     3121 cgcttcaagc tcacaaagca aacctggaag cgctcgggtc atcgctggaa aacgtgctga
     3181 aggtcacggt ctttatggcg gatgtgaaga ccgaaaagaa cgcgatgaat gaggcttacg
     3241 cgaagttctt tccgaaagac gccccggcgc gttcctgtgt gggcgtcgag ttccccgatt
     3301 cgcagacacg cgtagagatc gaactggtag cctggattcc cgactaatgt aattgaacat
     3361 tggcatccga tccgtgtgca tccgtggccc ttctcaatcg gttattagcc acggatggac
     3421 acggatgcac acggatagtt gtttttcgtt tcacaccacg ccccgctccc cctcattaaa
     3481 atatgcaaat cagttgcaaa acctctagca aaagagttag aggtccaata tactgtaatg
     3541 tacagtaatg cacgccaagc aacggagcga agccacaacg ttaagcgcca gcctggaaga
     3601 ttacctggaa gccatcttcc atatcgcttc ggaaaagcag gtggcgcggg cgaaagacat
     3661 ctcggcgcgc ctcggtgtga aaaactcctc ggtcaccggt gctttgcgga tcctgtccag
     3721 ccggggtctg gtcaactatg ccccctacga cctgattacg ctcacgccgg gtggagccga
     3781 agcggccagg gaagtcatcc gccgccacga ggcgctgcgc gacttcttcg ttaaggtact
     3841 caacatcgat ccggagaccg cggacgaagg cgcctgcaag atggagcacg ccattccgtt
     3901 gcccatcctg gaacgcttca tacaattcgt cgagtatttc gagacctgcc cctcggccgg
     3961 cgcacagtgg gttcgcgatt tcggctacac ctgccggggc ggcgccgagc gggagaactg
     4021 cgcgcagtgt gtttccgccg aagccgaaga acgaacgaaa ggaaaactta aagaaacgac
     4081 catgaagttg agcgatttga agatcggcca gcgagccagg atggtgaaac tcgcaggcca
     4141 cggggagttg cgcaagcggc tggtggacat gggaatggta ccgggcgcca ttctggcggt
     4201 cgagcgcgtg gcgcctctcg gcgatcccat cgacatcaag ctgcggggat atcatctttc
     4261 gctgcggaaa tcggaggccg cgatggttac cgttgccgcc attggagcgg ccgggcaggt
     4321 aacggcgtga caagctgcca ttcgcttcct ttgcctttgc accttgccgg tgggggaaga
     4381 tttgtgcgat tgcatcgcgt caccggaaac tccgaaacgc agagccgtct cgccgcgctc
     4441 gggttgatcc cgggggccga gttgcaactg gttggcggaa gcgcacgcgg tccgctgatc
     4501 gtcgggctca aaagcgggcg gctgatgctg gggcctgagt tggctcgtca tttatgggtg
     4561 gagtagctct ctttttttgc cgttgattta ggcgcgacgt acattgtacg ccgcgccgtg
     4621 aagggaaggc catgattgca gtcaagaaaa taaccgtcgc gctggccgga aatccgaact
     4681 ccggaaagac cacgattttc aacaatctga cgggcgcgcg ccagcacgtc gggaactacc
     4741 ccggcgtgac gatcgagaaa aaggaaggaa gctgctcgta tcgcggcgcg gaaatccagg
     4801 tagtggattt gcccgggacc tacagcctga cggcgttctc cccggaggag gtcgtcgccc
     4861 ggcggttcct ggtggaagag gcccccgatg ccgtggttgc agttctggac gccgcgaacc
     4921 tggagcgcaa cctctatctc gcgatgcagt tgctggaact ggaagtccct ctcatcctgg
     4981 actgcaacat gatggacctg gtgacgggcc ggggcgaccg gttggatctg cggcgccttt
     5041 ccgcgaaact cggcattccg gtagttgcca cggtcggcaa tcagcggcag ggcatggaag
     5101 aactgcggga ggccgtggcc agctccaatg gggtggaagg ccgcaaacag ctttcgccgc
     5161 gctattccga agacgtagag gcgtcgctgg cggcgctcga acaggagatt caaaacgcgg
     5221 cgccgcgctc gcgcttccca gctcgctgga ctgccatcaa gctgctcgaa aacgacccgg
     5281 ccgcgaacga agaatggcgc gggacctccg tcgcggagtt tgccgaacag gagcgtgcca
     5341 tgctcgagca agcgcgcggc gaaccggccg agatagtgct ggcggcggag cgttacgcgc
     5401 tgatcggcac catctgcgcc gattgttact ggcccgcggc ctcgccgcaa cgcagccgct
     5461 ccgacgtcat cgacgccgtg gtgacgcatc gcgcactggg cctgccgatt ttcctggcgg
     5521 cgatgttcgc ggtgttctgg gccacgttca atgtgggcgc gcaaccttcg gaatggatcg
     5581 aagtgggggt agcatggctt ggcggcgccg tctccggatg gtggccggcg ggctcggaaa
     5641 gctcgctcaa gtcgctgctg gtggacggca ttatcggggg cgtcggcaac gtaattgtgt
     5701 ttctgccgaa cattctgatc ctgttttttg cgattgccat gttggaagac tccggctata
     5761 tgccgcgcgc cgccttcatc atggatcgca tcatgcacaa gatcggactg cacggcaaga
     5821 gtttcattcc gctgttgctc ggattcggat gcacggtgcc ggccatcatg gcgacgcgca
     5881 cactggagag ccgccgcgac cgcctgactg cgattctggt ggcgccgctg atgagctgcg
     5941 gagcccggct cccgatttac tcgctgattc tccccgcgtt cttcccccag gtctggcagg
     6001 cgcccctgct gtggggcatc tacgtactgg gaatcgtcct ggcggtggtg atggcgaagc
     6061 tgctccgctt gaccctgttt aaaggagagc ccgccccatt tgtcatggaa ttgccgccct
     6121 accggatccc ttcgctgcgc gatggccttt tgcatacctg ggagcgctcc aggctctatc
     6181 tgaaaaaagc cggcaccatt atcgtgggga tttcgatcct gctgtgggcg ttgacgaatt
     6241 ttcccaagcc tccagccggc gccgaaaccg gcggctcgag cgagctggca tactcctatg
     6301 ccggccgcgc cggccacctg atggaacccg tgctcgcgcc gatgggattc gattggaaga
     6361 tcggcacggc gctggtgggg gccttcgccg ccaaagaagt tttcgtggcc cagatgggca
     6421 tcgtctacgc cgtgggcgaa ggcgaggagg ccgaagcgcc gctgcgcgag cagttgcgaa
     6481 atacttatcc gccgctggtt ggtttctgca ttgtgttgtt ctgcctgatc ggtacgccgt
     6541 gcatgacaac gctcgcggtg acgcggcgcg aaggcggcga gtggcgatgg gcccttttgc
     6601 aattcggggg acttaccgtg ctggcgtacg ttgtcaccgc gatggcgtac cagatgggaa
     6661 agctgtactt cggttagttg aggagtattt tcataatgga aaccgtcata gtcatcttcg
     6721 ttgtggccgc cgctgccgtg ctgcttctcc gcggcttata ccgtaatgca aaaaccggca
     6781 agccgatgtg cggctgtgcg tcggccggcg gcggctgtca gatgtcgaat tcgtgcgggc
     6841 agtcgcgtgt gaagaaggtc acgacacatg gacaaccggc agcttcttga gcagagctgg
     6901 gaagaactcc aggctcaaat ggaaaaacac cggcacctgg tccgcagcgc gcttgaaggc
     6961 tgcgccaccg gccgtacgga gtgtttatgg tgcgattgcc ctcaccgccg gacgctgctg
     7021 aacctggcgg cgcactcggt gaaggtgctg gacgatactc gcaaggcatt caaatcgaaa
     7081 caactggaag cgctccgcaa agatcttttg cgggtgctga ccgaggaagt gcgaagcggg
     7141 caaaccgcta acggccaacc tcgggagtaa tctacctagc cggccggaga atactccggt
     7201 cgggcactta tagttaccta cggtccggta attggccgga aagtgaggcg cactcggaat
     7261 gaagagttcc ggggagcgcc ttttttttgc ctacaaggag agcatatgaa acgcagttct
     7321 acaattttcc tcctggtggt cttatggctg gcggtggcaa gcgctgccag gcgcacagca
     7381 gggagtggta cagcagacgg ccgaaactcc ggagatggag cttccagtcc ttctccatca
     7441 gctcgaagag cgcggcatta cgctcagcct gggctatacc gccgagatct tttccgccgt
     7501 gagcggcgga gtagggcgtg gacgcaggag cgactacctg ggcagtttcg cgccgagcct
     7561 gacgctggat ttctcgcgca tcggcttcgg gcgcgggcag ttctatatga gcgggcaaac
     7621 gctgcatgga cgcgggatca gcgaatcccg catcggcacc gcacaggcgc tcagcaacct
     7681 ggaggagcag cgttttggca ggtttatcga agcctggtat gcggattcct accgggacgg
     7741 gcttctgaag gtcaaaatgg gacggcaata tgccgacgcg gatttcggag tggtggaaaa
     7801 cggcgccgat tttctgaact cttcctacgg cctgaatccc accacgccga tgccaacgtt
     7861 cccggctccc gaactggggg cgagcgtctg ggtggcaccg gcatcctggt tttcagccgg
     7921 tgccggggtc ttcaagggcg gcacactgga tgccctgacg gaggatggcg ccgccatgaa
     7981 ccgccgtcca ttcgcgatgt tcgaggccag gttcgagccg ttcagcaaga acgcggcgtc
     8041 gcacggacaa tatagtatcg gtctgtggcg gcaatcgcgc ggggcatggc tttccgcggg
     8101 cgaggagaac gccgcgccgg tgcgcaacta tggcatctat gcgactgcgg atcactggtt
     8161 catgcgttct ccgtcgggcg agcaccgtgg cccgggactg tttttccagt ggggatggac
     8221 accggcggat cgcaacgaaa tcaccggcta cacgggcggc ggcgtggcat gggcgggcct
     8281 gattcccgcc aggcgccggg acagcattgg catgggtgtg acccgtgcac gtctggttgg
     8341 gagggacagc gagaacatca tcgagtggtt ctataaactc caggtgagct cgaaactgat
     8401 ggtgcaaccc gatcttcagt gggttctgaa cccgtgcggc gatcatcgca gagcgctggt
     8461 tgccggattg cgcctgggcg tggagttcta acggctccgg ccgccggtgt gcagcacgtc
     8521 cgtcgcccgg caagttaaga tatcgcctta aggagttaac agatgtttcg ttttattctg
     8581 ctcgttctgg ctatatcgtt gactgcttcg gcgcaggatc cgcttctgac aaaggcacag
     8641 cggattttca aaccggtacc ggcgtctccg cccgcgcttc gcgccaatcc cattacgccg
     8701 gaaaagattg aactcggccg gatgctctat ttcgatccgc ggctctcgtc gagctggctc
     8761 atcagttgta atacttgcca caacgtcggg ctgggtggtg tggatctgct tgaaacctcg
     8821 gtcggccacg ggtggcagaa aggaccgcgt aatagcccga cggtattcaa cgcggtcttc
     8881 aatatcgcgc agttctggga cgggcgcgcg cgggatctga aggagcaggc acaggggccc
     8941 gtgcaggcgg cggtggaaat gaacagtaat cccggccgca cggtgaagac actgagcagc
     9001 attccggaat atgtcagccg ttttgagaag gcgtttccgg gcgaagctgc gccggtcagc
     9061 tttgagaaca tggcaaaagc gatcgaggca ttcgaggcga cattgacgac gccgggggct
     9121 cccttcgacc ggtatctgaa aggcgacgca aaggccatca atgaggagga gaagaagggg
     9181 ctccaggttt tcatggcgaa aggttgtacc gcctgccatg ccggtgtgaa cctgggcggg
     9241 cagggatact tcccgttcgg tgtggtcgaa aagcccggtt ccgagattct gccgccgcag
     9301 gacaaaggcc ggtttgccgt gacgcgcacg gcgagcgacg aatatgtgtt caaatcgccg
     9361 tcgctgcgca acattgcatt aaccgcgcct tacttccact ccggcaaggt ttgggatctg
     9421 aagcaggcgg ttgccatcat gggcactgcc caactgggcg cagaactcaa ccaggccgaa
     9481 acggaggcga tcacccggtt tctccatacg ctaaccgggg agcagccgaa gatcgaatat
     9541 cccattctgc cgccgcacac cgcgtcgact ccgctgcccg aggtcggcgt gggtccgcgg
     9601 ccggccgggc actaatcagc gcgtggtcgc ttcatcgatc ccgtcgggcc tggggccata
     9661 ccagggcagc ggctggcccg cttcgtaagc gccgatatcc ggagctttgc cgagaaagcc
     9721 gtcgttgatg ccgggaatgc ggagaccggc atccacaaca ggcgagccgg ggcgcgggcg
     9781 caggtccggc ggagtcatca gggtgagtgg cgagacggga aattccacgc cgtcgaagac
     9841 gctcatatcc acgcgcactc cgtggggctc aacctcgaaa aagtccaatt tgccaatcgt
     9901 ggccttgaag ggcgtctggt acgtgcccac cgcgtcataa tcgaagctcg aattcggccc
     9961 atgtgaattg atctgggccg ccatcccgga gcccccgcca taaccgcccc atctgacgcc
    10021 accactaggg ccgccgatcg aaagattatt ccggaaccag gcgtgatcga acggtactcc
    10081 cgaaaaacat gccattccat cgccggcctt cacggttgta ttgtgcgcga tcacgtcgcc
    10141 gcggctgccg cgatgcaact taaacggcac ataagtgagg ttatacatga cgtttctcaa
    10201 aaagtaagtt ggtccgccca agcttggctg cgaactaagt ccgacaaagg agttagtaat
    10261 ccggttgcgc atcacgcggc agttgtggaa acagaaatcg gcctcgatcg cgtcgtctgc
    10321 accaacgtag atgtcgttat tgtaaatgtc tatcgagatc tgctcggtaa cattctcttc
    10381 ttccatgttt gagatgcaat cgcgaaagcc ggtaacccga ttgaagcaga tgacgttgcc
    10441 gggtccggta agttgaatgc cttctccgat attcttaccg cttgcgccca tggcttcatt
    10501 ggtccagggc gtagggccat cgatgatgtt atccgagatg tagcagttct ttgctcccgg
    10561 agccttcgat gcccgtacgc cgtacgttgc ccgaatacgg cagcgcctca ccacacaatc
    10621 ttcggcgcgg accatgctga ccgcggtccc gccggggttc tctatcgcca ggttttcgag
    10681 atacacgtgc ttgcggccgt cgagcgaaat cgactcgaac acggccttcc cgtccgtaga
    10741 gcggtaaacg atggggcgcc ccggctcgcc atcggcctcc ggcgcgaaac gaccgtagga
    10801 accggcttcg agtactacga tttcgcccgg gcgcgcgccg ccaatggtcg ccgggttggc
    10861 ctttcggact ttcgctcccg ctggagcgcg agggacgggc cgcgtcctga cgctgagggt
    10921 tcgcgtcgcg ccgccgccat cgggatcgtg caatatgagg cggacctcat aggaggttcc
    10981 gggctccaga tcgaagatac ttccggaatg cttgttgtcc caatgaaaaa tgggccgggt
    11041 gccgctgctt gacccagccg ggacgcgccg aagcggcatc ccctcgcgcc accgggcctc
    11101 gccggatttg cggtagctga ctttcacaac gctgttcagg tttgcatctc cctcaatcct
    11161 ccactccagc gcaagagcgg taacggtcgg gtagggagca gagacctcgc cgggaaccgt
    11221 tgcatccggc gattgcagca ataggagtac taagaaaaga agatacacgc ccgattttaa
    11281 cagtgccgcg ccgaatcgat tcgagtttga aagcaagaac cggagtgtct cgaaaaagag
    11341 ccgctgataa atttgtagtt gaaaggcgaa cggtgacgat gaacgattca acgaaaaatg
    11401 cccggcgtcg agatgcaacg gtgaacgatg cccgttgggc ctctgtgttg gcacgggacg
    11461 caggggcgga cggccaattc ttctactccg tcgacaccac cggtgtatac tgccgccctt
    11521 cctgcgccgc ccgcagggcc cggccggaaa acgtaagctt tcacctcacc agcgaagacg
    11581 cgaagaaagc cgggttccgt ccgtgcaagc gctgccgtcc ggaccagctt ccggttgccg
    11641 gaccgcacgc cgcgaaaatt gcggcggtgt gccggatcat tgagaactcg gaggtgatgc
    11701 ccactctcga acagttgtcg cgacaggcgg ggttgagtcc gtatcacttt caccgggtgt
    11761 tcaaggcaat gaccgggctg acgccgaaaa agtacgccgc ggctcatctg gagaagcgcg
    11821 tccgccgcgc gcttggatcg agcgccacgg tgacggaggc gatttacgat gccggctaca
    11881 actcgaacgg acggttttac gaaacatcga atgaagtgct gggtatgaca ccttcgagct
    11941 atcgcgcggg cggggcccgc acgcagatac gtttcgccgt tgcggaatgc tctttgggat
    12001 cgatcctggt cgcgcaaagc aagcgcggca tttgcgccat tcttctcggc gacgatccgg
    12061 aggtgctgac acgccatctg caggaccagt ttccacgggc aactttcact ggcggcgatg
    12121 ccgggttcga ggaactggtc tcgaaagtgg tgggcctcat cgaaacgcct gcattgggcc
    12181 tggacttgcc gctcgacatt cgaggcaccg cttttcagca gcgcgtttgg caggcgttgc
    12241 gcaaaattcc ggttggctcg acggcgagct atgcggagat tgcccggaag atcggctcgc
    12301 cgcgctcggt ccgcgcggtt gctcaagcct gcggagcaaa ccttcatgcc gtagccattc
    12361 cgtgccatcg cgtggtgcgg accgacggcg ccttgtcggg ctaccgctgg ggcgtggaac
    12421 ggaaacgtgc gcttcttgaa cgggaagcga ggacctgaac gaaaagggtg ggttcgttac
    12481 agggcggccc gcccgcgctc ccgccatttc cgtatcgctt ccgtgtgctt tgggtagtgg
    12541 ctgtaggtgt ccagacgcag gcggcggcga aaacgcgttt cgttcgtgaa ctggctctcc
    12601 ggcgcgctat cgatatatgc aaggagccgg gtgtgcgtct cttccagctg ccgcaggaca
    12661 tcggagagcg aagcgtccct cttctgctcc gtcattcgag cgttgaaggc atggatgccg
    12721 ccgtacgtcg cggagtacgg tggcggcctc tcgcttttga gaatgagcgg caggtttttc
    12781 agagcctcct cttcccacgt ggttacatgg gcgaggatgt cttttacgga ccagccgccg
    12841 gtgaccccag gctccaacat ctgcgagtcc gataagccgg cgtaggattg acggaactcc
    12901 tgccatgctt tgtcgagccg tttcagaagt tgccgtctat ccacgtcgaa tgtatttgat
    12961 aatacacatc tgcgggcggg aatgcatatg aagaatatcg gggaacgcct ggaagcggtc
    13021 gattgggagc gagtgtggag agatctcgat gagcagggca gtgccgtcat tgggagtctt
    13081 ctctcgcccg gagaatgcag gctgatcgcc ggcctctatt cggcggacgg tccattccgc
    13141 agccacgtcg tgatggcgcg gcatgggttc gggcgcggtg agtacaagta tttcaaatat
    13201 ccgctacctg ctcttgtcgc ggaactgcgg accgccatct atccgcatct tgtgccggtc
    13261 gcgaatcgtt ggaacgaggt gatgaatacc ggtgttccgt atccggaaag gcacgcggac
    13321 ttcctcgcac gctgccatca ggccggccag gtcaagccga caccccttct acttcaatac
    13381 gaagcgggcg attacaattg cctgcaccag gacctctatg gggttcatgt cttcccgctc
    13441 cagaccgcca ttttgctgtc tgaacccggc aaggacttta ccgg
//
LOCUS       DASTMK010000331         6299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_304288, whole genome shotgun sequence.
ACCESSION   DASTMK010000331 DASTMK010000000
VERSION     DASTMK010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304288"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /gene="nifJ"
                     /locus_tag="VFQ79_14425"
     CDS             <1..2377
                     /gene="nifJ"
                     /locus_tag="VFQ79_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEU0140915.1"
                     /translation="FSTEPDNVVRAMFYGLGADGTVGANKNSIKIIGENTDNYAQGYF
                     VYDSKKSGSVTVSHLRFGPQPIRSSYLISRANFVACHQFTFLERLDVLKNAEPGAVFL
                     LNSPFGPDEVWNQLPSKVQRQIIDKKLRFYVIDGYTVARETGMGARINTIMQTCFFAI
                     SGVLPREEAIAAIKDSIKKTYGRRGEAVVQKNFAAVDSALDHLHEVQIPAQVTSTFEM
                     RPPVPAAAPAFVQDVLASIIAGEGDSLPVSKLPIDGTFPTGTAKWEKRNIALEIPVWD
                     EKLCIQCGKCVLVCPHAVIRAKIYESDKLMGAPETFKAVPARWREFKDLKYTLQVAPE
                     DCTGCGLCVDVCPVKDKTTPKRKAVNMEPQPPLREQEAANWDFFLGLPELDRTLLNTG
                     QVKDVQILEPLFEFSGACTGCGETPYIKLMTQLFGDRAVITNATGCSSIYGGNLPTTP
                     YTANCDGRGPSWSNSLFEDNAEFGMGVRLAIDKQSEFAREMVPQFSAELGDELVAGIL
                     GADQTKEKGIQEQRERVALLKQKLAGIDTPAARNLLAVADALVKKSVWILGGDGWAYD
                     IGYGGLDHVLASGRNVNILVLDTEVYSNTGGQMSKSTPKGAVAKFAAGGKPTAKKDLA
                     MLATTYGNVYVARIAMGAGDMHTLKAFLEAEAYDGPSLIIAYSHCIAHGYDLVHGLEQ
                     QKKAVNSGHWPLFRYNPLLAKEGKNPFVLDSKAPSIPLEQYVYNETRYTMLRHSDPEA
                     AKVLLQQAQEDIQTRWKLYEARAAQNGGPKEPTPAGPKQEQKETVKETQNA"
     gene            2370..3380
                     /locus_tag="VFQ79_14430"
     CDS             2370..3380
                     /locus_tag="VFQ79_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEU0140916.1"
                     /translation="MLDLSTTYLGLRLKNPLVASSGPLCKDVGNLLKLEDAGVSAVVL
                     HSLFEEQINLESQELDRFLSEPAEQYAEALSYFPDMTDYNIGPDGYVEHIRRAKEALD
                     IPVIGSLNGISTGGWIKYAKLIEDAGADALELNIYYLPTRMDLGGTEVEKMYADLVSH
                     VKASIRIPVAVKLGPYFSSIPNVARQLDACGADGLVLFNRFYQPDFNLEDLEVVPNLV
                     LSNSHELLLRLHWVAILYGNVKADIAITGGVHTAEDVVKSMMAGARVAMMTSVLLRHG
                     VDYAETLQTSLVEWMMDHEYESVGQMQGSMSARSVSSPAAFERANYMKVLSSYTLRAA
                     AR"
     gene            3556..4185
                     /locus_tag="VFQ79_14435"
     CDS             3556..4185
                     /locus_tag="VFQ79_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0140917.1"
                     /translation="MKKPRILLADNHTILFEGLKSLLSRQFELVGADCRSLITSAAEL
                     KPDIIVMDISIPEWNGVEAARQLRESGCPSKIVFLITHVDPGLLRETFRVGMSAYVSK
                     RSAVWELATAIREVLAGRSYITPLVTEDIMHSFVESAVRGKAVKLSRRQKEVLQLIAE
                     GKSHKEIAATLGISLKTVEFHKRHIIQELGISNVAELTKYAIKHGIVEA"
     gene            4293..5549
                     /locus_tag="VFQ79_14440"
     CDS             4293..5549
                     /locus_tag="VFQ79_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU0140918.1"
                     /translation="MRLPLDHLMGQPMLAPLMKSVNGIGLLNLIREQGPISRAELAKL
                     SALSKPTVSSQVESLIRKNLVVERGPGKSGHRGGKKPTLIEFNADFGRLIAVEIDASE
                     IRTALTDLEGKVIDRATVVTKPETGSGQVLRRVVRGIEKLLNAPERREKLRVIGVAAP
                     GRVDAERGIVLEAGNLFGWRNVLLRQRLQKSFGVPVFVDNDVNMAALGEKAHGMAQSV
                     SNFVLVRLDTGIGSGVVIDGKLHHGVNWAAGEIAHFVLDPAEAGKDWRARGYLESYVG
                     ADRIAERVRAATRASRGKLSQLVKNKGEFPALLEAFRQGDAEAGRILREVTTRIALAV
                     AHLSAAYDPSMVVLQGALLELLFEKIRQTVARVIPWQQNIALSTLGEEAVLRGTVVAA
                     RQHVYELIARTLDGRGDETVTGVIEA"
     gene            5567..>6299
                     /locus_tag="VFQ79_14445"
     CDS             5567..>6299
                     /locus_tag="VFQ79_14445"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0140919.1"
                     /translation="MRKWIFLYLILALAVPAAWPQASTSTVNGTVSDETGAVIPGADV
                     TLLNKGTNGRSRTTTNEVGFYIFPGITPGAYLLTVEMAGMQKFEGTISVQVQQTVAVN
                     AVLKPGTTTTIIEVQDVTPIMVTDVPTLGHVLERQRIEQLPINGRSLTSLLVTVPGME
                     GNRAYGMREGSQEMVLDGAALTDRLWGGNPRRQPALDSVQEFKVENNSSSAKFNRPTS
                     IVLSTKSGTNGFHGSLFETHRNNAIG"
BASE COUNT         1368 a         1788 c         1905 g         1238 t
ORIGIN      
        1 tttctcgacc gagccggaca acgtcgtccg cgccatgttc tacggcctgg gcgcagacgg
       61 cacggttggc gcgaacaaga actcgatcaa gatcattggt gagaataccg acaactacgc
      121 tcagggctac ttcgtttacg actcgaagaa atcgggatcg gtaaccgtgt cccacttgcg
      181 ctttggccct caaccgatcc gttcgagcta cctcatcagc cgggccaact tcgtcgcctg
      241 ccaccagttc accttcctcg aaagactcga tgtgctgaag aacgccgagc ccggcgccgt
      301 gttcctgctg aacagcccgt tcggccccga tgaagtgtgg aaccagttgc cgtccaaagt
      361 gcagcggcag attatcgaca agaagctccg gttctacgtc atcgacggct acacggtagc
      421 cagggaaacg ggtatgggcg cccgcatcaa caccatcatg cagacctgct tcttcgccat
      481 cagcggtgtg ctgccgcgcg aagaggctat tgcggcgatc aaagactcca tcaagaaaac
      541 ctatggacgc cggggcgaag ccgtggtgca gaagaatttc gctgcggtgg acagcgcgct
      601 ggatcacctg cacgaggtcc agatacccgc gcaggtgacc agcaccttcg agatgaggcc
      661 tccggtaccg gcagcagcgc cggcattcgt ccaggacgtg cttgcctcca ttattgccgg
      721 ggaaggcgac tcgctgccgg tcagcaagct ccccatcgac gggacgttcc cgaccggaac
      781 ggcaaaatgg gaaaagcgta atatcgcgct cgaaatcccc gtctgggacg aaaagctctg
      841 catacagtgc ggcaagtgcg tactggtgtg cccgcacgcc gtcattcgcg cgaaaatcta
      901 cgaatccgac aagctgatgg gagcgcccga gaccttcaag gccgtcccgg cacgctggcg
      961 ggagttcaag gatctgaaat acacgctcca ggtggcgccg gaagactgca ccggctgcgg
     1021 gctttgcgtg gacgtctgcc ctgtgaagga caagaccact ccgaagcgca aagctgtgaa
     1081 catggagccc caaccgccgc tgcgcgagca ggaggcggcc aactgggatt tcttcctcgg
     1141 cctgcccgag ctcgaccgga cgctgctcaa caccggccag gtgaaggatg ttcagattct
     1201 ggagccgctg ttcgagttct ccggcgcttg caccggctgc ggcgaaacgc cctacatcaa
     1261 gctgatgacg cagcttttcg gcgatcgggc ggtcattacg aacgccaccg gttgttcgtc
     1321 catctatggc ggcaacctgc cgacaactcc gtatacggcg aattgcgacg gccgcggccc
     1381 ctcgtggtcg aactcgctgt tcgaagataa cgcggagttc gggatgggcg tccggcttgc
     1441 catcgacaag cagagcgaat tcgcccggga gatggttccg cagttttccg ctgaactcgg
     1501 cgacgaactg gtggcaggca tcctcggtgc ggatcaaacc aaagagaaag gcattcagga
     1561 gcagcgcgaa agagtggctc tgttgaagca gaagctggca ggcatcgata caccggcggc
     1621 ccgtaacctg ctggccgtgg cggatgcgct ggtgaaaaag agtgtctgga tcctgggcgg
     1681 tgacggctgg gcttacgata tcggctacgg cggcctcgat cacgtgcttg cttccggacg
     1741 caacgtgaat atcctggtcc tcgataccga ggtctactcg aacactggcg gacagatgtc
     1801 gaaatcgact cccaagggtg cggtagcgaa gtttgccgcg ggagggaaac cgactgccaa
     1861 aaaggacctg gccatgctcg ccaccaccta cggcaacgta tacgtggcac gaatcgccat
     1921 gggcgccggc gacatgcata cgctcaaagc gtttctcgaa gccgaggcct acgatggtcc
     1981 atcgctgatc atcgcctaca gccactgcat cgcgcacggc tacgatcttg tccacgggct
     2041 cgaacagcag aagaaagccg tcaattccgg acactggccg ctgttccgct acaacccgct
     2101 gctggccaaa gagggcaaga atccgtttgt cctcgattcg aaagcgcctt ccattccgct
     2161 ggagcagtac gtgtacaacg aaacgcggta tacgatgtta cgccacagcg atccggaagc
     2221 tgcaaaggtg ctgctgcagc aggcgcagga agacattcag acccgctgga aactgtacga
     2281 ggcccgcgcg gcgcagaatg gcggccctaa agagcccact cccgccggac cgaagcagga
     2341 gcagaaagaa accgtgaagg agacccaaaa tgcttgatct ctccactacc tatctgggtt
     2401 tgaggctgaa gaatccgctg gtggcttcct ctggcccctt gtgcaaggat gtcgggaacc
     2461 tgctgaagct ggaagacgcg ggggtatcgg cggttgttct gcactcgctt ttcgaggagc
     2521 agatcaatct cgagagccag gaactcgacc ggtttctttc cgagccggcc gagcagtatg
     2581 cagaagcgct ctcctacttt ccggatatga cggactacaa catcggtccg gatggctatg
     2641 tggagcatat acgccgggcg aaagaggcgc tggatatccc ggtgatcggc agcctgaacg
     2701 gcatttcgac cggcggctgg atcaagtacg cgaagttgat cgaggatgcc ggagcagacg
     2761 ccctggaatt gaacatctac tatctgccta cccgcatgga tctgggcggg acggaggtag
     2821 agaagatgta cgccgacctg gtatcgcacg tcaaggcgag catcagaatc ccggtggccg
     2881 tgaaactcgg gccgtacttc agctcgattc ccaacgtggc ccggcagctt gatgcctgtg
     2941 gggccgatgg actggtgctg ttcaaccgtt tctaccagcc ggatttcaat ctcgaggatc
     3001 tggaggtggt gcccaacctg gtgctcagca actcgcacga attgctgctg cgcctgcact
     3061 gggtggccat tctctacggc aacgttaagg cggatattgc cattaccggc ggtgtgcaca
     3121 cggcggaaga tgtagtgaaa tcgatgatgg ccggcgccag ggtggcaatg atgacgtcgg
     3181 ttttgctgcg gcacggagtg gattacgccg aaactctaca aacgagtctg gtcgaatgga
     3241 tgatggatca cgaatacgaa tccgtcgggc agatgcaggg cagtatgagc gcccggtccg
     3301 tgtcgtcgcc ggcagcgttt gaacgcgcca actacatgaa agtactcagc agttacaccc
     3361 taagagccgc ggcacgatag taaatctctc ctctgaacag tcccggggaa cgtagggtgt
     3421 ttcccgggac ctggttttca ctttggtatc tacggctacc gctccctcgc ggtcgcggct
     3481 cagaaaccgg ttatttgtac agcagaaaat ttttgtctat ttttcggtaa aatcagtcta
     3541 tttattaact ggactgtgaa aaaaccgcgg atattactgg ctgataacca tactattttg
     3601 tttgaagggc tgaagagcct tctgtcgcgg caattcgagc tggttggagc cgactgccgt
     3661 tccctgatta catccgctgc agagctgaaa ccggacatca ttgttatgga tatatccata
     3721 ccggaatgga acggtgtgga ggcagcacgg cagctccggg agtccggctg tccatcgaag
     3781 atcgtcttcc tgataacgca cgtggacccg ggtctcctcc gggagacgtt ccgggtgggg
     3841 atgtcggcct atgtatcgaa gcgctcggcc gtatgggagc tggctacggc aatccgcgag
     3901 gtactcgccg gacgctctta catcacgccg ctggtgaccg aagacatcat gcattccttt
     3961 gtcgaatcgg cggtgcgggg gaaggccgtg aaactcagcc ggcgtcagaa ggaagtactt
     4021 cagttgattg ccgagggtaa gtctcacaag gaaatcgccg ctacactggg tatatctttg
     4081 aaaacagtag agtttcacaa gcgccacatc attcaggaac tcggcatcag caatgtcgcc
     4141 gaattgacga agtacgcgat caagcacgga atcgtcgaag cctgacccgg gcgccgcccc
     4201 cccccgccat ctccctcagc ctctggcttc cgattgactt gcttcccgta tcacggtata
     4261 taaggtaagt taactaacta tctatcgatg aaatgcggct gcctctcgac catctaatgg
     4321 gccagccgat gctggcgcct ctgatgaaga gcgtgaacgg gatcggtttg ctcaatctca
     4381 ttcgcgagca aggccccatc tcgcgagcgg aactggccaa attgagcgct ttgagcaagc
     4441 cgaccgtgtc gagccaggtg gaaagcctga tccggaagaa tctggtagtg gaacgtggac
     4501 cgggaaagtc cggccaccgc ggcggtaaga agccgacgct gatcgaattc aacgctgatt
     4561 tcggccgtct tattgcggtt gagattgatg cctcggagat ccgaaccgcc ctgacggatc
     4621 tggaaggaaa ggtcattgat cgcgctaccg tcgttaccaa gcctgaaaca ggttccgggc
     4681 aggtccttcg ccgcgttgtg cggggaatcg aaaagcttct gaatgcgcca gaacgacgcg
     4741 aaaagttgcg cgtgattggt gtcgcggctc cgggccgtgt cgatgcggaa cgggggatag
     4801 tgctcgaggc aggcaacctc tttggctggc gcaacgtgct gctcaggcaa cggctgcaaa
     4861 agagtttcgg tgttcccgtt tttgtcgata acgacgtgaa tatggccgcc ctcggagaaa
     4921 aggcgcacgg catggcccag agcgtctcca atttcgttct ggtgcggctc gacaccggta
     4981 tcgggtcggg cgtggtgatt gacggcaagc tgcatcacgg tgtcaactgg gccgcgggtg
     5041 aaattgcgca cttcgtgttg gatccggccg aagccggcaa ggactggcgg gcgcggggtt
     5101 acctggaatc ctatgtgggc gccgatcgca tcgcagagcg cgtgcgggcg gcgacgcgcg
     5161 catcgcgagg caagctttcg cagcttgtca agaataaagg cgaatttccc gcgctgctgg
     5221 aggcattccg ccagggagat gccgaggccg gccgcatcct gagagaagtg actacccgca
     5281 tcgcgctggc ggtggcgcat ctttccgctg cctacgatcc ttccatggtg gttttgcaag
     5341 gtgcgctcct cgaactcctg tttgagaaaa tccggcagac agtggcccgc gttatcccgt
     5401 ggcagcagaa tatcgctctc tcaacgctcg gggaagaggc cgtcctgcgg ggaacggtgg
     5461 tggcagcccg ccagcatgtc tacgagttga ttgcaaggac cctcgatggg aggggcgatg
     5521 agacggttac aggtgttatc gaagcttaaa aaaggagaag ttgcggatga gaaagtggat
     5581 ttttctatat cttatactcg cgctcgccgt tcctgcggcc tggccgcaag ctagcacgag
     5641 cacggtaaat ggcaccgtat ccgatgaaac cggtgcggtc attccgggcg cagacgtgac
     5701 cctgttaaac aagggcacga atgggagatc ccgtacgaca acgaacgaag tcgggttcta
     5761 tatctttccc ggcatcactc ccggcgccta tcttctgacg gtcgaaatgg ccggtatgca
     5821 gaaattcgag ggcaccatct cggttcaggt acagcaaacc gtagcggtca atgccgttct
     5881 gaagcccggc acaacgacca cgatcatcga agtacaagac gtcactccca ttatggtcac
     5941 ggacgtcccc actctgggcc acgtcctgga acggcagcgc attgagcagc ttcccatcaa
     6001 cgggcgctcg ctgacaagcc tgctggttac cgttcccggc atggaaggta accgcgccta
     6061 tggtatgcgg gagggctcgc aggagatggt gcttgacggt gccgcgctga cagaccgtct
     6121 ctggggcgga aatccgcgcc ggcagcccgc ccttgattcc gtccaggaat tcaaggtcga
     6181 gaacaacagc tcttcggcga agttcaaccg gccgacctcc attgtgctct ccacgaagag
     6241 cggcacgaac gggtttcacg gctccctgtt cgagacgcac cgcaacaacg ctatcggca
//
LOCUS       DASTMK010000332        24376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_367906, whole genome shotgun sequence.
ACCESSION   DASTMK010000332 DASTMK010000000
VERSION     DASTMK010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24376
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367906"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..3195
                     /locus_tag="VFQ79_14450"
     CDS             238..3195
                     /locus_tag="VFQ79_14450"
                     /inference="COORDINATES: protein motif:HMM:NF039257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tectonin domain-containing protein"
                     /protein_id="HEU0140920.1"
                     /translation="MKVRITAVVAFLTISWQTASGQSISWDRQSGLALHISGNAGGSL
                     WVVGVDRQNEMGFTVHKFNGTNWVRNYSIVPSRRLAVNPLDQLWITDDSGALYFQSGT
                     SPLLMDRKANDVGVGGDGSVWIIGTDQRPGGYGIYTRTGLTWTNINGGAVRIAVAKDG
                     APWVVNDAGTIFRYNTAQARWEVKPGKARSIHTGAASGAVWMLGAEPMAGGYPIFQWN
                     PATQGWDPYGSYGAVEMTEVAGTPWIVQSDGSIYSKAPNIIISTINITLTPTLPSPTP
                     QAQAPVRITGSGKLICAAADSGSATYCGNTKADYVGAYTLDTTCDEGFYDMIYGGSCW
                     KCPDETDGRGGWIRSADAVDKDTACWRVPKETTSHAIKVRSPAWAWDCPSGSFWDGYS
                     PDGIGGSCWRCPDDNPRRTLAAVWASNACASTLNETRPATLLTFNGCPTPNAANMNLP
                     GKRSPGKPFLDIAAGWSQGVASGGCYACPVVDETGNFLITARNADPLYDKENNQGCRI
                     NMKWQPPVFYEPGLAYMQGVKDVIWEQRLFDGDEITGFLYDLAEGQELGDATPAARQW
                     VIARWQEIAQKPYNSETFRSLVFARLKNALKKDVDNRTPGEKKLIESFANYVRDRRIY
                     LAEQGLAMYDAWKAHSDGIRSSMRQSPLGEAFYYGTVPLDFNGTLSSLIGMGGVGASV
                     AGTLYAANLFTKGVEVVQTFNRFGEPVGHACSRSTSIWGMSHGLTIFKSLQGLKVVSG
                     ASLIQVAFTILTSIAIDQFMAIESARPKLEASLAEAKQPVDLNILVKGANGEDMLYFY
                     WAKAMDTTDGEDPQVVQLAAEAHARAQQKGYQAPPKQTVEITMEPVSVGDRVSSGTGT
                     VSAFSSPGLTQGQQLVSPNGKFQATMQADGNFVIYTEKRPIWATNTQGRENPPYRLAM
                     QPDGNLVVYGSPDKDVTLSGGVCGPKSACIAIWNTGQRSGTAPFTLTMQDDGNLVVYD
                     NSNRAVWASGTNR"
     gene            3711..6554
                     /locus_tag="VFQ79_14455"
     CDS             3711..6554
                     /locus_tag="VFQ79_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0140921.1"
                     /translation="MTIEKWREVLSCYQAAIEVPSERRREFLESAGRDPEVIGRALRM
                     IEALQQDKSELTSGSPTAEWQLRTGTTVGHYDVVAPLGQGGMGEVYIGRDTALDRAVA
                     LKFLRPEQAGSPAAAERFVREAKAASALNHPNIVTVHEVLQAHNSLVIVMELVEGRAA
                     RQLCGAPVPLDTLLNVGTQTALALAAAHAHGIVHRDVKPENIMVRTDGYVKLLDFGLA
                     RRIALDKSTAGGLPAGTLRYMSPEQIRGESLTPASDIFSLGLVLYELAAGRHPYAEYS
                     TLDGAVAIVNEPIPDISSSNPPPPPSLAFLIGAMLSKEPGRRPAAAKVVAALRSIGEL
                     PATGHAPGTVSLPGTAGALRKPWVWPAMAVALLGLVVFSGLFWPRDPRSSKLPELRSW
                     PLTSNAGWEESPVFSPDGESVAFRWTRKLNEPWQIYVKRLDADDPVQLTHPKPGENIG
                     PIAWSPDGNSIAFKAAFTVEGRGAIYTIPKQGGEVAKLVDLFNANFSSSIDWSPDGTQ
                     VVFSDASPATQQLAIYTFNVHTGEKRKLTSPPAGDWGDWDPQFSPDGRMLAFKRVQGF
                     WSDAIYLMPASGGPARRVTNGSGGIWGHAWMPTGNGLIVSTQRDGSVFGLWRFPLTPG
                     AAPVRITGGGLDAITPASGRGVGRVAWVNRLDDTNVYRVPMAEAGAPTALITSTRRDE
                     RAYYSPDGRIAFASDRSGSWEIWIARSDGSNQARVTSFGGPLVGFPRWSPDGRHLAFE
                     TKALGRGRIFVLECQSENARCGGAKPLTAGAGAETSTDALPSWSADGAYVYFTSNRTG
                     RDEIWKQALAGGAAIQVTRRGGYASYESRDGKWLYFSKLKPYSIWRMPGSRAKVAAES
                     AELLIGPPVDLTPTGWTVTSEEILFIGVSDNVPFRAIRGYRLATGEIRTLLNLPEISE
                     RGTDLSASPDSRWLLYGQLDRSGSNIVIAETGQ"
     gene            complement(6570..7523)
                     /locus_tag="VFQ79_14460"
     CDS             complement(6570..7523)
                     /locus_tag="VFQ79_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0140922.1"
                     /translation="MDSANSTKNRRKWTRRSYLGGAGLAGAAAALYGVAPWFWHRYTR
                     EMTRPVASPRHRPRPQDWPDRGIQVAWLGHSTVLLKIDGFTILTDPVLSTRAGIHLGP
                     ITLGIKRLIEPALELEDLPLIDLVLVSHAHMDHLDTWSLARLENRHRSMVMAPSTSDL
                     VRVRRYKEVRELKWGEEARFGPVTVKAFQVNHWGARMRSDTYRGYNGYTIESGKRRVV
                     FAGDTADTHLFKSLRSSKQYDLAIMPIGAYNPWIRYHCTPEQALRMANEANAERLIPV
                     HHQTFELSREPGTEPIERFMAAARSEPERVVIREIGQEFRS"
     gene            7634..8092
                     /locus_tag="VFQ79_14465"
     CDS             7634..8092
                     /locus_tag="VFQ79_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140923.1"
                     /translation="MKLLVLFCLLIAGAPHLAGADAGGRADYVGGTLASLDGNCSGRI
                     ITSDSLSMIFDTKDGPVRVPYEKINLLEYGQKVDRRYAMAVIVSPMLLLSKKRQHFLT
                     VGYQDDDGHQQAMILRIHKKDVRAVLVSLEARTGLKIQFQDDEARKAGKG"
     gene            8089..8697
                     /locus_tag="VFQ79_14470"
     CDS             8089..8697
                     /locus_tag="VFQ79_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140924.1"
                     /translation="MRRAGFVLAALGAFAQFAPAQPDPAELDRQMLEQLLGVKRVYIE
                     RLGGGEAADQIRDMIISSFQRAKLFIVTENADRADAVLRGSAEDLVFTDTFQSSDSIN
                     ARAAVGNSTTKTADNPGRRGSLSVGVGQQESTRIAERKHEASAAVRLVGKDGDVIWST
                     TKESLGAKFRGASADVADKITKQLMADYERARNLKAGSAPSP"
     gene            complement(8689..8913)
                     /locus_tag="VFQ79_14475"
     CDS             complement(8689..8913)
                     /locus_tag="VFQ79_14475"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU0140925.1"
                     /translation="MDLRVKVILKSSKSEVVGYLPDGTLKVKIAAAPERGKANAELCA
                     LLAKQFGVPRKNVVIVSGETSQHKHVRISR"
     gene            complement(8915..10189)
                     /locus_tag="VFQ79_14480"
     CDS             complement(8915..10189)
                     /locus_tag="VFQ79_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140926.1"
                     /translation="MRIAAVLCIFAAAAAANQNFEFRVDAPAEIVADLTLSSPGADWA
                     SEGREAAVARVVADNHPEQYVTVYAGPEQHTYSVFLGKFDAGRHKLRIERDTQLSAKG
                     AGLEIGKVEFRTATPGEPNFAVFAHAPVLFARQDTIGRFTDIPLLMYCERLNENGRPY
                     LQYTIIFSNEDGGTSTRALMARWGRTTDIEYVYKAFLNDDGTLIRGTIQAKDHEEIEF
                     RGKFFGTHPLLTVSTINNMVSDAEPTNVRYQLVPVDADLSNRTRESVMDEHPITWSVM
                     AKELIREQKLRPYGTVEGQKISDPRNYLYVDVKAAFQSARLKLIARRQGDHWMATSDL
                     GQLEYAVGRDGWFRTTLELPPGTIPETIEEIGVECLVAPVPPGKPLPEGGLCKVDQIA
                     AFMLDEEYRPRPVLMESKPAIEIRTGSILLLR"
     gene            complement(10189..10614)
                     /locus_tag="VFQ79_14485"
     CDS             complement(10189..10614)
                     /locus_tag="VFQ79_14485"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEU0140927.1"
                     /translation="MPAVIIAALAIAGGALFWYLEREPEPSQAPSLTAEAKEYVRSLG
                     LSDVEMSRTGNYVGQDIIEITGKIANNGDRQLQQVEINCVFYDAYSQLVLRERVAIVR
                     ARDGGLASGGTRNFRLAFDNIPASWNQNMPQLVIANIQF"
     gene            complement(10643..11986)
                     /locus_tag="VFQ79_14490"
     CDS             complement(10643..11986)
                     /locus_tag="VFQ79_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU0140928.1"
                     /translation="MSLQELAGEVVRLALNEGATDAECTISEGEEFSASVRMGEVESL
                     KEAGAQGAGVRVLKGKRSGSAYTSDLSRDGIARMVSSALELAAITSEDPHAGLPEASE
                     LGAIDGDLALYCDDVAHLETPFKIEQARQAEKAALDFDSRIANSEGASFDSHAGARYF
                     ANSRGFSGSYRTSSCSMSAVPVASDGEKMERDYWFSVARSYSRLEEPAYIGRKAAERA
                     LRRLGARKVPTQRVPVVFEPRTARSMLDHIFEAVSGDSIYRKASFLTDKIGEQVAAEQ
                     LTAIDDSTIPGLFGTSPFDDEGVPSRRTVVIERGVLKSYLLNSYTARKLGLKTTGNAA
                     RGLSGNAGIGHGNLFIDNGAQKPEDILRSIKQGLYVTELIGFGVNIVTGDYSRGAVGL
                     WIENGELAYPVSEVTIAGNLGEMLKGIEAVGSDLEFRGSVAAPTLMIREMTVSGH"
     gene            complement(11983..13413)
                     /gene="tldD"
                     /locus_tag="VFQ79_14495"
     CDS             complement(11983..13413)
                     /gene="tldD"
                     /locus_tag="VFQ79_14495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEU0140929.1"
                     /translation="MGLQDSFFGGKFGITERDLERYLGEALSAGGDYADLYFEYVATS
                     SIGIDESIIKSANQGVSLGVGVRVISGERTGYAYSDDLSPEKIMHAARVAAHIASGPA
                     KVDKIGLEEKQGRNLYPIVTAPADTDLKERAALVERADRAARAYDARIFQVQAAYADS
                     LRHVLVATSDGSISYDRQPLARLSVAALAKANGGTPQRGYSGGGGRIALEYFQTERTP
                     EFFAQEAARQAVMQLAAVEAPAGEMTVVLGPGWPGILLHEAVGHGLEADFNRKGVSAF
                     SNRIGQQVASPLCTVVDDGTIHNRRGSLNMDDEGNVTQRNVLIENGVLRGYLQDKLSS
                     RILGSASTGSGRRESYQHIPMPRMTNTFMLPGESEPEDIIRSVPRGLYCSNFGGGQVD
                     ITSGNFVFSASESYLIEDGKLTRPVRGATLIGNGPEALKYVSMVGNDLKLDEGIGICG
                     KEGQSVPVGVGIPTIKIDKMTVGGTA"
     gene            complement(13669..13905)
                     /locus_tag="VFQ79_14500"
     CDS             complement(13669..13905)
                     /locus_tag="VFQ79_14500"
                     /inference="COORDINATES: protein motif:HMM:NF019493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140930.1"
                     /translation="MKTIALPDHLSERLEKVASETSQSIASLAEEAIETYLDQTARIR
                     ESIAQLERGEGIPYERLRPWLQNLMAGQRNPSPK"
     gene            complement(14018..15316)
                     /locus_tag="VFQ79_14505"
     CDS             complement(14018..15316)
                     /locus_tag="VFQ79_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0140931.1"
                     /translation="MRILAAMLCANAMFAQANYDLLLKGAHVIDPKNKLSAVRDVAIR
                     DGLIAAVERNIPAAQARKVVDVAGLYLTPGLIDLHAHVFAGSEGKGLAGGHASIFPDN
                     FALRTGVTTVVDAGSSGRHNFAQFKRTVIDRARTRVLVFLNIGGAGMPGDAQEQDLEE
                     MDAKAAANLAIANRDTIVGIKVAHYRGPDWTPVERGVEAGTLANIPVMIDFGQFRPER
                     PFQELVLKKLRPGDIYTHAFYVPVPMLDDNGRLLPYLFEARKRGVLFDVGHGGAAFEF
                     RQAVPAVKQGFPPDVISTDIHSGSINAGMKDQLNVMSKFLNMGMTLDDIIYRSTWNPA
                     RIIHREQLGHLSVGALADIAVLRLEKGQFAFVDSWGARMDGTQRLLCELTVASGLVVW
                     DLNGRTRERWDKLGNYQPQGEPYWNGTRGGARPKPLPLSR"
     gene            15401..16588
                     /locus_tag="VFQ79_14510"
     CDS             15401..16588
                     /locus_tag="VFQ79_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140932.1"
                     /translation="MKSTRRRLLQTAALSPAAAAAAATGNLTVSSPSPLYASLGIKPV
                     INGVGVVTVLGGSIMPPEVIRAMEEASRYFIPLPELQRKVGMRIAELLKAPAAMVTCG
                     AASAITVGTAACLSEGDSAKLRRLPNREGIRYEVIQQKSHRSGYEHQMELCGAKIVTV
                     ETRKDLEAAINEKTGMMFFLNKADPDGQVKAEEFVKIGKERGVPTMNDAASDATPKEN
                     LWKYTEQGFDLVIFSGGKALRGPQASGLLLGRKDLIEASYPAMSPYGGIGRGMKVGKE
                     EMCGLLAAVERYLKVDHEAEYRELDRRVASIRQALQGVSGVQTERHVPVIANEVPHVK
                     VLWDESARGLSSQGVVEKLRDGDPPIHIQRPGNGQLLISVWMMRGTEHEIVGRRLKEI
                     FST"
     gene            complement(16654..16914)
                     /locus_tag="VFQ79_14515"
     CDS             complement(16654..16914)
                     /locus_tag="VFQ79_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140933.1"
                     /translation="MMGALKEALPSGRKVLNRAATNDNRSQFISEAIMYDVNSKAKNT
                     LTERLKEGAITNAQRDLEIAREWFSLDEEAWKPATPTRKRTK"
     gene            complement(16930..17217)
                     /locus_tag="VFQ79_14520"
     CDS             complement(16930..17217)
                     /locus_tag="VFQ79_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140934.1"
                     /translation="MQSVEINGVRCLQEPTKRVKPDGESEVIGTTCVSPELGILLVYE
                     DIKVTVGGENLRMVSELENMQMDTEPPAEWFQIPPDYRLIPGHPGRPATQK"
     gene            complement(17240..17575)
                     /locus_tag="VFQ79_14525"
     CDS             complement(17240..17575)
                     /locus_tag="VFQ79_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140935.1"
                     /translation="MRYRFCNSIVLGCWAFLMPAVLSSQTEPSRHPPYIAVSKRTIYI
                     AEPGKPEVLKDIVRKTEARDSQGRRYSSGASIVPERFRYDWVRDFVSGRSYEVNRQQK
                     VAYFTNHRF"
     gene            complement(18110..18316)
                     /locus_tag="VFQ79_14530"
     CDS             complement(18110..18316)
                     /locus_tag="VFQ79_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140936.1"
                     /translation="MGTTCVFPELGNLTVHQDHKIDVQGERLHIVSELENMQMDTEPP
                     SEWSQIPPDFQLITGYAGKLAPMN"
     gene            complement(18534..20321)
                     /locus_tag="VFQ79_14535"
     CDS             complement(18534..20321)
                     /locus_tag="VFQ79_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU0140937.1"
                     /translation="MSVLLFWRRFGRVLRIIRASKPAPGFEIRPVSRRVLDFIGEVML
                     QAKVIRERPMAGIAHAWVFWGFCVFALVTLDHIASGFGFDLISRESSFGRLYFGWAAA
                     FAVAVSVGIAGLAVRRFLVRPKWLGELSGESGIIAALIFFLMVTYLAEWWAHEDSAFQ
                     RANWWAHTLALLVFLPLIPHTKHLHLVLSPFTIFLRRGSFSRIPPLEGDEDFGLFMGK
                     DFTRIDSLQAFSCVECGRCTEHCPANNTGKILNPKQVVLGLRSYLRGPGTAGEEPVLD
                     NHVSMEAVFQCTSCGACEYQCPVGVEHLPMIVGLRRGAVNTGRWEDEHGTRFFLNLER
                     NGNALGMSASERDKFIQKAGLPLFDGSQEFCLWLGCMGAYDPRGREIVQSLVKVLEHL
                     GITYGVLKREKCSGDPARRLGNDLLFGELAEFNLEQMREHGVKRLVSICPHCVETIQQ
                     DWKEYGASFPIEHHSELLFRNRSRLPSNGGSRQIVFHDPCYLGRYRDIYEEPRSIARL
                     GGHVIDPPRSRHRSFCCGAGGGLAFLGEEKGKRVNVERAEELVATKADVIGAACPFCN
                     TMFRDALSSMEASPQLLDIAQIAAASIEK"
     gene            20437..21396
                     /locus_tag="VFQ79_14540"
     CDS             20437..21396
                     /locus_tag="VFQ79_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEU0140938.1"
                     /translation="MRFVISGAAGFIGSHLCDRLIAEGHSVLALDNFITGSPANLAHL
                     EGADGFEFREHDVTSPIEASAPVDFVLHLASLASPKDYLNHPIETLETGSTATRNMLE
                     LARAHRARFLLTSTSECYGDPLEHPQAESYWGNVNPVGIRSCYDESKRYAEALTMAYH
                     RVHGLRTNIARIFNTYGPRMQLNDGRVVPAFVDQALRGEALTVFGDGKQTRSFCYVSD
                     LVEGLYRLMFSDERYPVNLGNPDEMTILEFAEHVRKITAAAAPIRFEPLPSDDPQRRR
                     PDITKARTLLDWEPRVSLEDGLRETIAYFRGLYASSVPVRSLE"
     gene            complement(21311..21832)
                     /locus_tag="VFQ79_14545"
     CDS             complement(21311..21832)
                     /locus_tag="VFQ79_14545"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU0140939.1"
                     /translation="MKKFKWSESSSVAVNARTVLPEFFSAFYERAEPVLKSGDPQALH
                     DFRLAAKKFRYSLEMFRGVHGPGLAQLASLMKELQDQLGAISDCSTTRAMLAESDSPE
                     FVAWLEARSARRIEEFRRFWQERFDKAAAQKWRNYLATYPRDASTRENAQEQKRRTSR
                     GNRRSSRGVRPPG"
     gene            complement(21829..22263)
                     /locus_tag="VFQ79_14550"
     CDS             complement(21829..22263)
                     /locus_tag="VFQ79_14550"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU0140940.1"
                     /translation="MTKSSGAENIRVYTLSQISSLLDRVVYHVSRAAKSPDEKRVHEA
                     RVSIRRFVQALRFFRQFIPGEPSKRIRKRLKSIMNLSAEVRSRDIALHLLEESEAPDR
                     TGVRKRMELERKVSMKELAAALRRLNRRNYSVKWRESLRLEA"
     gene            complement(22260..23798)
                     /locus_tag="VFQ79_14555"
     CDS             complement(22260..23798)
                     /locus_tag="VFQ79_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEU0140941.1"
                     /translation="MPKYAAVDIGSNSVRMQAAEVIPGSPVRVLAAERDVTRLGESVF
                     RTGRISPEALEFTCSQLRKMAAIYRQLDVAGIRAVATSAVRDASNQREFLERASEAAG
                     AAVEVISGQEEARLIQLGVESRWPHKNRRTLVIDVGGGSAELIVSENGRIVEAFSKPL
                     GAVRLTSVFLTHDPPTAKELHQLEEYIQEKIGAAVARIGRRRMDRVIATSASAAAVVG
                     SINRVARSKREAADKLRATTPQVRKFYREISGLDLAARKKVRGIGPRRAEIIVPGAAV
                     FLKILEALRLPSIYYSSAGLRDGIIADLAARGVGRERAQLTPEQRRSVEEMARRYGVP
                     VKHARKVAALGRSLFDSLAPVHRLPLETGRLLEAAAYLHDVGHFVSDVGHHKHSHYLV
                     SNSDMPGFTAWERKMTALLCRYHRKSLPAPRHDIFQALTPEERQTVMRLTPMLRLADG
                     LDRSHDQRVEGIACQMRNGGVTVKVESAVDTDLEMWAAERVAELFEQVFALQLTITRA
                     RKQT"
     gene            23833..>24376
                     /locus_tag="VFQ79_14560"
     CDS             23833..>24376
                     /locus_tag="VFQ79_14560"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140942.1"
                     /translation="MPLFAANRYKGKLIVVEGIDGSGKSTQISLLSQWLRSNGIAVAF
                     SEWNSSPLVRETTKRGKKKEMFTPSTFSLIHATDFADRMERYIIPLMKAGAVVCADRY
                     AYTAFGRDVVRGVSRNWVRNLYRFAVRPNVAFYFRVPLDVALGRILGGRDAIKYYEAG
                     MDLGLSRNVEESFRIFQGRII"
BASE COUNT         4937 a         7266 c         7147 g         5026 t
ORIGIN      
        1 tagagggcgt ccaccaattg cttcgccgcc tgctggtctc cccggcggaa ggaacgcatt
       61 aatgcgctga tatcccctgg aacaagagcc gacatttttc aagcgtgccg ccatcataac
      121 aaacaattcc gtcaattttt aagctcctgc gtggaaccac acgccgtggt ctcatgccat
      181 ccggatgata aagaccaacc ccgtcctcgc cctatatagg gacagagggg acattacatg
      241 aaagttcgca tcaccgcggt tgttgctttc ctgacgatat cgtggcaaac ggcgtcgggg
      301 caatccatct cctgggacag gcaatccggt ttagcactgc acattagcgg caacgccggg
      361 ggttcactct gggtggtggg agtggatcgc cagaacgaaa tgggattcac cgtgcacaaa
      421 tttaacggca caaattgggt tcggaattac agtatcgtgc cgagccgccg gctggccgtc
      481 aacccgctcg atcaactctg gataacagac gattccggcg ctctctattt ccaatcgggg
      541 acctctccgc ttttgatgga tcgcaaggca aacgatgttg gcgtgggcgg cgatggctcc
      601 gtctggatca tcggtacgga ccagcgcccg ggaggctatg ggatctacac gcggactggc
      661 ctcacctgga ccaatatcaa cggcggggcg gtgcgtattg ccgtagcgaa agacggcgcg
      721 ccgtgggtgg taaatgatgc aggcacgata ttccggtaca acacggctca ggcaaggtgg
      781 gaggtgaagc ctggcaaggc acgctcgatt cacacaggcg ccgctagtgg cgcagtgtgg
      841 atgttaggcg ccgagccaat ggccggcggt tacccgattt ttcagtggaa tcccgcaacg
      901 caaggctggg acccgtacgg ctcctatggt gcggtggaaa tgacagaggt ggcgggtacg
      961 ccctggatcg tgcaatccga tggatccatc tattccaaag cacccaacat tatcatttca
     1021 acaatcaaca tcacgcttac gccgacgctg ccgtcgccga caccgcaggc gcaggctccg
     1081 gtgcgaatta ccgggtcggg caagttgatt tgtgccgctg ccgacagcgg ctcagcgact
     1141 tactgcggca acacgaaggc cgattatgtg ggggcctata ctttggacac gacctgcgac
     1201 gaagggtttt acgatatgat ttacggcggc tcctgctgga aatgtcccga tgaaaccgac
     1261 ggcagaggcg ggtggattcg gtctgccgat gcggttgata aggacaccgc ctgttggcgg
     1321 gttccaaagg agacgacatc ccacgcgatc aaagtgcgat cgccggcatg ggcctgggac
     1381 tgtccgagcg gatcgttttg ggatggatat tcgccggatg gcatcggggg gagttgctgg
     1441 cgatgtcccg acgacaatcc acgcaggacg ctggccgctg tctgggcaag taatgcctgt
     1501 gcctcaactc tcaatgagac gcggccggct acgctgttga cgttcaacgg ctgtcctaca
     1561 ccaaatgccg cgaacatgaa tctgcctggc aaacggagtc cgggaaaacc gtttctcgat
     1621 atagcggccg gctggagcca gggagtcgcg agcggtggat gttatgcctg cccggtcgtc
     1681 gatgagaccg gcaactttct gatcaccgcc cgtaatgccg accccctgta cgacaaggag
     1741 aacaaccaag gctgccgcat caatatgaaa tggcagcctc ctgtctttta tgaacccggc
     1801 cttgcctaca tgcagggagt aaaggatgtg atctgggagc agaggctgtt cgatggcgac
     1861 gagatcactg ggttccttta cgatctggcc gaggggcaag agctgggtga tgccaccccc
     1921 gcagcccggc aatgggtgat tgcgcgatgg caggaaatcg cgcaaaagcc gtacaacagc
     1981 gaaacgttcc ggagcctcgt gtttgctcgt ctgaagaacg ctctcaaaaa ggatgtagac
     2041 aacagaacac cgggggagaa gaagctgatc gaatccttcg cgaactatgt tcgcgatcgg
     2101 cgcatttacc tggccgagca ggggctggca atgtatgatg cctggaaagc ccacagcgat
     2161 gggatcaggt catcgatgag acaaagtccg ctcggcgaag cattttacta tggcacggtt
     2221 ccactcgact tcaacggaac gctcagttcg ttaattggaa tgggcggcgt cggcgccagc
     2281 gtggccggaa ccctctatgc ggcgaatctg tttacgaaag gtgtcgaagt ggtacaaaca
     2341 ttcaacaggt tcggtgaacc tgtcggtcac gcctgctcac gatcgacctc catttgggga
     2401 atgtcgcacg ggttgaccat atttaagtca ttgcagggtt taaaagtggt ttcaggcgca
     2461 tcgttgattc aggtggcctt taccatcctg acctcgatag cgattgacca gtttatggcc
     2521 atcgaaagcg caagacccaa gcttgaagcc agccttgcgg aggccaagca acctgtcgac
     2581 ttgaacatcc tggtaaaggg agccaacggc gaagacatgc tctactttta ttgggcgaag
     2641 gccatggata ccaccgacgg tgaagatccg caggtggtcc aactggctgc cgaggctcat
     2701 gcgagggcgc aacagaaggg ataccaggct ccaccgaaac aaaccgtcga gatcacgatg
     2761 gagccggttt ctgtcggcga cagagtctct tcgggtacgg gaaccgtttc tgcgttcagt
     2821 agccccggtt tgacgcaggg ccaacaactg gtctcgccga acgggaaatt tcaagcgacc
     2881 atgcaggccg acggcaactt cgtgatttac acggagaagc ggccgatctg ggccactaac
     2941 acccagggga gagagaatcc gccctacagg ctggcgatgc agcccgacgg caacctggtg
     3001 gtgtacggat ccccggacaa ggatgtgacg ctttccggcg gcgtgtgtgg tccgaagtcg
     3061 gcgtgcatcg caatttggaa caccggccag cggagcggaa cggcaccatt tactctcacg
     3121 atgcaggatg acggcaacct cgtagtctac gacaactcga atcgcgctgt ttgggctagc
     3181 ggcacaaacc gttgaccttt ccgccggcgg cagcccgact gagccgcaaa tgttgattgg
     3241 ggcgcaaact tatccgattt cggtcaccgg cagtgcccga aatccattcg cagtcgcccg
     3301 ccggcgtcca ctacaaaatc cacttcgcgg gttcggtccc gccagaaaaa gaggctgcct
     3361 gcgcgtccag cacgaggttc acgaccccgg agttgggcga aaacaaacgt ctcccataca
     3421 gcccctgccg caggggacgg cagtagagtt tcttccgagc ggatgttcaa caacgtgcac
     3481 aggagacccg tgtcggcaac gtagagcttt ggacttttga cgatagactt gctccgattc
     3541 gcgaaccaag gttcaagcag gatgacctga ccggacgcct ccagcataga cagccaatgg
     3601 ttccctttct ggtggggccg aagagcgctt cgcccccttt tacatccctc gcacccgact
     3661 cgcctcctgc gaagctcgtc gattccagat ataatgcgca ctaatcagcc gtgactatcg
     3721 agaagtggcg cgaggtcttg agctgctatc aggctgccat tgaggtgcct tcggaacggc
     3781 ggcgcgagtt tctggaatcg gccggaaggg atcccgaggt gatcgggaga gccttgcgga
     3841 tgatcgaggc actccagcag gacaaaagcg aactgacatc cggttccccg actgcggaat
     3901 ggcaactgcg gacggggacc actgtgggtc attacgacgt cgttgctccg ttgggccagg
     3961 gcggaatggg ggaggtatac attggccggg ataccgccct ggatcgagcg gtggcgctga
     4021 agtttctgcg tcccgaacag gcgggctcac ccgcggccgc cgaaagattc gtacgcgagg
     4081 ccaaagccgc ctcagccctg aatcatccga atatcgtgac ggttcacgag gtgttgcagg
     4141 cccacaactc gcttgtgatc gtcatggaac tggtggaggg acgggcggcc cgccaactct
     4201 gtggcgcgcc ggtgcctctc gatactctcc tgaacgtggg cacacaaacg gcactggctc
     4261 tggctgccgc ccacgcgcac ggcattgtcc atcgtgatgt gaagcccgag aacatcatgg
     4321 tccgtaccga cggctacgtg aagttgctcg atttcgggct cgcccgccgt attgcactgg
     4381 ataagagcac tgccgggggt cttcccgcgg gcacgctgcg atacatgtct cccgaacaga
     4441 tcaggggaga atcattgact ccggccagcg acatcttctc gctcggcctg gtgctctatg
     4501 aactcgcagc gggccggcac ccgtacgcgg agtactcgac gctggatgga gctgtggcga
     4561 tcgtaaatga accaattcct gatatcagtt cgtcgaatcc gccgcctccg ccaagcctcg
     4621 ccttcctgat cggcgccatg ttgtcgaaag aacctgggcg gcgtccggcg gccgcgaaag
     4681 ttgtcgcagc actgcgcagt atcggagaac ttcccgctac cggtcatgcg cccggcaccg
     4741 tatcgctgcc aggaactgcc ggagctttgc ggaagccctg ggtttggccg gctatggcag
     4801 ttgctctgct tgggctggta gtcttttccg gcttattctg gccacgcgac ccgcggtctt
     4861 cgaagctgcc agagcttaga tcctggcccc taacctccaa cgccggatgg gaagaatcgc
     4921 ctgttttctc gccggatggt gagtcggtgg ctttcaggtg gacccggaag ctgaacgaac
     4981 cgtggcagat ctatgttaaa cggctggacg ccgatgaccc ggttcaattg actcatccga
     5041 agccggggga gaatatcggc cctattgcct ggtcgccaga cggaaacagc attgccttta
     5101 aagctgcttt cacggtggag ggacgaggtg ccatttatac gataccgaaa cagggcggcg
     5161 aggtggctaa actcgtcgac ttgttcaacg caaacttcag ctcctcaatc gattggtcac
     5221 cggatggaac gcaagtcgtg ttttccgacg cttcgccggc cacacagcag ttggcaatct
     5281 ataccttcaa tgttcatacc ggtgagaagc gaaagctgac ttctccaccg gccggagatt
     5341 ggggcgattg ggatccccaa ttttcaccgg atggcagaat gctggctttc aagagagtgc
     5401 aaggattttg gtcggatgcc atctatctga tgcccgcatc cggggggccg gccaggcggg
     5461 taaccaacgg ttcaggcggc atctgggggc atgcctggat gccgaccggc aacggactga
     5521 tcgtatcgac tcaacgcgac ggcagtgtct tcgggttatg gcggttcccg ctcactcccg
     5581 gggctgctcc ggtgcgtatc acgggtgggg gtctggacgc gatcacgccc gcaagtgggc
     5641 gtggagtagg cagggtcgcc tgggtaaacc ggttggatga caccaacgtt tatcgagttc
     5701 ccatggcgga agccggcgcg cccaccgcgc tcattacatc gacgcggcgc gacgagaggg
     5761 cctactactc gcccgatggg cgcatcgcct ttgccagcga ccgctctgga agttgggaga
     5821 tctggattgc ccggtccgac ggctccaacc aggcccgcgt caccagtttt ggaggccctt
     5881 tggtgggttt tccccgttgg tctcccgacg ggcggcatct ggcgttcgaa accaaggcgc
     5941 tcggccgcgg aaggattttc gtgctcgaat gccaatccga aaacgcgcgc tgcggagggg
     6001 ccaaaccact cacggcagga gccggcgcgg aaacctccac cgacgctcta cccagttggt
     6061 cggcggatgg agcatatgtg tacttcacat caaatcggac gggccgcgat gagatatgga
     6121 agcaggcgct tgcgggcggt gcggccatcc aggttactcg ccgtggcggc tacgcgtcct
     6181 acgagtcccg tgatggcaaa tggctctact tttcgaagct gaaaccttat agcatttggc
     6241 gcatgccggg ctcgcgggcg aaggtagcag cagaatccgc ggagttgctc atcggtccgc
     6301 ccgttgacct gactcccaca ggttggactg tgacttccga agaaattcta ttcatcggcg
     6361 tcagcgacaa cgtaccattc agggcgatcc gcggctatcg cctggccacg ggagagattc
     6421 gtactctcct gaatctaccg gaaatttccg aaagagggac cgatctttcg gcctcgccgg
     6481 attcacgctg gctgctctac gggcagctcg atcgatccgg cagcaacatc gtgatagcgg
     6541 agacagggca gtaaagagaa gtacggtttt cagcttcgga attcctggcc gatctcccgg
     6601 atcaccacgc gctccggttc gctgcgcgcc gcggccatga aacgctcgat aggctccgtc
     6661 ccaggctcgc ggctgagttc aaaggtctga tggtgaaccg ggatcagccg ttcggcgttg
     6721 gcctcgttcg ccatgcgcaa cgcctgctcc ggcgtgcagt gataccggat ccagggattg
     6781 tatgcaccga tgggcataat cgcgagatcg tattgcttcg agctgcgcag gcttttgaac
     6841 aggtgtgtgt cggctgtgtc gccggcgaac actacgcgcc gcttgccgga ttcgatcgta
     6901 tatccgttat atccgcggta ggtgtcggat cgcatccggg cgccccagtg gttcacctgg
     6961 aaggccttta ccgtcaccgg gccgaagcgg gcttcctcgc cccatttcaa ctcgcgcacc
     7021 tctttgtatc gccttacccg caccagatcg cttgtgctcg gggccattac catcgaccgg
     7081 tgccggtttt ccagccgcgc gagcgaccag gtgtcgagat ggtccatatg cgcgtgcgaa
     7141 acaagaacca ggtcgatcaa cggaagatct tccagttcga gggccggttc gatcagcctt
     7201 ttgatgccga gtgtaatagg tcccaggtgg atgcctgcac gcgtgcttaa aacgggatcg
     7261 gtaaggatgg tgaagccgtc gattttgagc aggaccgtgc tgtgccccag ccaggcgacc
     7321 tgaatgcctc ggtccggcca atcctgcgga cgaggcctgt ggcgagggct cgccaccggg
     7381 cgtgtcatct cacgggtgta ccggtgccag aaccagggtg ccactccgta gagggcggcc
     7441 gcggcgccgg ccagtcccgc tcctcccagg taactccttc tggtccattt ccgccggttt
     7501 ttggttgaat tcgcagaatc cacgcctttt tacagtatcc cttgttatta cattggatgc
     7561 agttcccgga ccggccgttg ccatgagcat tccgcctgag aattttaccg gctttccacg
     7621 actattcaca cgcatgaaac tcctggtcct gttctgtctc ttgatagcgg gtgcgccaca
     7681 cctggcaggc gcggacgcgg gcgggagggc cgactacgtg ggcggcacct tggcctcgtt
     7741 ggacggaaat tgctctggca ggatcattac ttcggattcc ttgtcgatga ttttcgacac
     7801 caaagacggg ccggttcgcg tgccttacga aaaaatcaat ctgctcgaat acggacagaa
     7861 agtggaccgc cgctacgcga tggctgtcat tgtttcgccg atgctgttgt tgagtaagaa
     7921 acgccagcac tttctcaccg tgggctatca ggacgacgat ggacaccagc aagccatgat
     7981 tctgcgtatc cacaagaagg acgtgcgcgc tgtactcgtc agtctggagg cccgcaccgg
     8041 cttaaaaatc cagttccagg acgacgaggc ccggaaggca ggcaaaggat gaggcgggcc
     8101 gggttcgttc tggcggcgct gggcgccttc gcgcagttcg ctcccgcaca gccggacccc
     8161 gccgagttgg accggcagat gttggaacaa ctgctcgggg tgaagcgtgt ctacatcgag
     8221 cgcctgggcg gcggggaggc agccgatcag attcgcgaca tgatcatcag cagcttccag
     8281 cgcgcaaagc tgtttatcgt cacggaaaat gcagaccgcg ccgacgccgt attgcgtgga
     8341 tcggccgaag acctggtatt cacagacacc ttccaatcca gcgattccat caacgcgcgg
     8401 gcggcagtcg gaaacagcac cacgaaaacc gcggacaatc ccggcaggag ggggagcctc
     8461 agtgtaggcg ttggccagca ggaatcgacg cgaatcgccg agcgcaaaca cgaggcgtcg
     8521 gctgccgtcc ggctggtcgg taaagatggg gatgtgatct ggtccactac caaagaaagc
     8581 cttggcgcga agttccgcgg cgcgagcgcc gacgtagccg acaaaatcac aaagcagttg
     8641 atggcggatt atgaacgcgc gcgaaatctg aaagcgggtt ccgctccatc accttgagat
     8701 gcgcacgtgc ttgtgttgtg aggtttcacc gctcacaata accacgttct ttcgaggcac
     8761 gccaaactgt ttcgccagca gcgcacagag ttcggcgttt gctttgccgc gctcaggcgc
     8821 cgccgcgatc tttaccttga gcgtgccgtc gggcaggtag ccgaccacct cgctctttga
     8881 gcttttcagg attactttca cgcgcaggtc catttcagcg taacaggagg atggaaccgg
     8941 tgcgaatctc gatggccggt ttcgattcca tcagaaccgg ccgcggcctg tattcctcgt
     9001 ccaacataaa agccgcgatc tgatcgacct tgcatagccc gccctccggc aatggctttc
     9061 cgggtgggac aggcgcaacc aggcactcga caccgatttc ttcgatggtt tcgggaatcg
     9121 ttccgggcgg cagttccaga gtggtgcgga accacccatc ccggccgaca gcatattcca
     9181 actggcccag gtcggaggtt gccatccaat gatccccctg ccggcgtgca atcagcttca
     9241 gcctggcgct ttggaacgca gcctttacat ccacgtagag gtaattgcgg ggatcggaga
     9301 tcttctgccc ttcgacagtc ccatacggcc tcagtttctg ttcacgaatg agttccttgg
     9361 ccatgacaga ccaggttatc gggtgctcgt ccatcacact ctcgcgggtc cggttcgaga
     9421 gatcggcatc caccggtaca agctgatacc gcacgtttgt cggctccgca tccgagacca
     9481 tattgttgat cgtgctgacc gtcaacaagg gatgcgttcc gaagaatttg ccccggaatt
     9541 cgatttcttc gtggtccttg gcctgtatcg tgccgcggat cagtgtgccg tcatcgttga
     9601 gaaaggcttt gtagacatac tcgatatcgg tggtgcggcc ccatcgcgcc atcaaagccc
     9661 gcgtggaagt gcccccatct tcattcgaga aaatgatggt gtattgcaga taggggcggc
     9721 cgttttcgtt gagccgctcg caatacatca acagcggtat atcggtaaat cggccgatgg
     9781 tgtcctgtcg cgcgaaaagc accggcgcat gggcgaaaac cgcgaagttc ggctcccctg
     9841 gcgtagcagt ccgaaattcc actttcccga tttccagccc ggcgcccttt gccgacaact
     9901 gcgtgtcccg ttcgattctc aacttgtgcc ggcccgcgtc gaattttccg agaaacacgg
     9961 aatacgtatg ctgttccgga ccggcataca ccgtcacgta ttgttcgggg tggttatcgg
    10021 caacaactcg cgcgaccgcg gcctcacggc cctccgaggc ccaatcggct cccggagagg
    10081 acagggtgag atccgccaca atctccgcgg gagcatccac gcgaaactcg aagttctggt
    10141 ttgccgccgc cgcagcggcg aaaatgcaca gcacagccgc gatgcgcatc agaattgtat
    10201 gttggctatg acaagctggg gcatattctg gttccagctc gccggaatgt tatcgaatgc
    10261 gaggcggaaa ttccgtgtgc cgccggacgc caggcccccg tcgcgggccc gtacaatcgc
    10321 cacgcgttcg cgcaggacaa gctggctgta agcatcataa aaaacgcagt tgatttccac
    10381 ttgctgcaat tggcgatcgc cgttgttggc gatcttgccc gtgatctcga tgatgtcctg
    10441 gccgacataa ttcccggtgc ggctcatttc gacatcactc aatccgaggc ttcggacgta
    10501 ctctttcgct tcagcggtca gcgagggggc ttgggacggc tcaggttcac gttccaggta
    10561 ccagaacaaa gcgccgccgg caatcgccag ggcagcgatg ataacggctg gcactacaga
    10621 tcggctgcga tttcttaccg tgctagtggc cactgacggt catctctcga atcataagcg
    10681 tgggagcggc tacggaccct cgaaactcca gatccgagcc cacggcttca atgcctttca
    10741 gcatctcgcc cagatttcca gcgatggtga cttccgaaac cgggtaagcc agttcgccat
    10801 tctcaatcca caatcccacg gcgccgcggg agtagtcccc cgtaactata tttacaccga
    10861 acccgatcaa ctccgtgaca tagaggccct gcttgatgga ccgcaggatg tcctcgggct
    10921 tctgcgcgcc gttgtcgatg aacaggttcc catgcccaat ccccgcattt ccgctcaggc
    10981 cgcgtgccgc gttgcctgtc gttttcagcc ccagctttct ggcggtatag gagttcagaa
    11041 gatagctttt caggacgccg cgctcaatca ccacggtccg gcgcgaaggc acgccttcgt
    11101 cgtcgaacgg agatgttccg aacaggcccg ggatggtgct gtcgtcgatg gcggtaagct
    11161 gctccgctgc cacctgctcg ccgatcttat ccgtgagaaa agatgctttg cggtagatgg
    11221 aatctccgct caccgcctcg aagatgtggt cgagcatcga tctcgcggtg cgcggctcga
    11281 ataccaccgg tacgcgctga gtgggaactt tgcgcgcgcc cagccgccgg agagcacgtt
    11341 ccgcagcctt gcgccctata taagccggct cttcgaggcg cgagtagctg cgcgccacgg
    11401 aaaaccagta gtcacgctcc attttttcgc cgtcggaagc cacgggaact gcgctcatcg
    11461 agcaacttga cgttctgtac gaaccggaaa aaccgcgcga gttggcgaaa tagcgtgctc
    11521 cggcgtgtga gtcaaaggag gcaccttcgg agtttgcaat ccggctgtcg aagtcgaggg
    11581 ctgctttctc cgcttgccgc gcctgctcaa ttttgaacgg cgtctcgaga tgtgcgacat
    11641 catcgcagta caacgccagg tcgccatcga tcgcacccaa ctcggacgcc tccggcaggc
    11701 cggcgtgcgg atcttccgac gtgattgcag ccagttcgag cgcgctcgaa accatgcgtg
    11761 cgatgccgtc gcgcgaaaga tcggaggtgt aagccgagcc ggaccgttta cccttcagca
    11821 ctcgcactcc ggctccctgc gcgccagcct cctttaggct ttcaacctct cccatccgga
    11881 cacttgcaga gaactcttcc ccttccgaaa tggtgcactc ggcatcggtc gcgccttcgt
    11941 tcagggccag gcgcaccacc tcgcccgcga gttcctgcaa gctcaagcgg tgcctcccac
    12001 cgtcattttg tcgattttga ttgtcggaat acccacgccc accggcacgc tctgtccttc
    12061 cttgccgcag atcccgatgc cttcatcgag cttcaaatcg ttgccgacca tgctgacgta
    12121 tttgagcgcc tccggaccat tgccgatcaa cgtagcgccg cgcacgggcc gggtcagctt
    12181 cccgtcttcg atgagatagc tttccgaggc cgagaagaca aagttgccgc tcgtgatgtc
    12241 cacttggccg ccgccgaaat tcgaacaata gaggcctcgt ggaacggatc gtatgatgtc
    12301 ttccggctca ctctctccgg gcagcatgaa cgtgtttgtc atgcgcggca tgggaatatg
    12361 ctggtagctt tcacgccgtc cgcttccggt gctggcgctt cccagaatac ggctggacag
    12421 cttgtcctga aggtacccgc ggagcacgcc attctcgatc agtacgttcc gttgcgtcac
    12481 gtttccttcg tcatccatgt tcaacgaacc gcggcggttg tggatggtgc cgtcatccac
    12541 aaccgtgcac agcgggctgg ccacctgctg cccgatgcgg ttgctgaacg cggaaacacc
    12601 tttccggttg aagtcggcct ccaggccgtg gcccacggct tcgtgcagga ggatgccggg
    12661 ccagccgggg ccgagcacaa cggtcatctc tccagcgggt gcctccacgg cagccagttg
    12721 cattacggcc tgccgggcgg cctcctgcgc gaagaactcc ggtgtccgct cggtttggaa
    12781 gtattcgagc gcgatgcgcc cgccgccgcc cgagtatccc cgctgtgggg taccgccgtt
    12841 ggctttggcc agcgccgcca cgctcagccg cgccagcggt tgccggtcgt aggagatcga
    12901 tccgtcgctg gtcgccacca gcacgtggcg caggctatcc gcataagccg cctgtacctg
    12961 gaaaatgcgg gcatcgtagg cgcgtgccgc ccggtccgcc ctttcgacga gcgctgcccg
    13021 ctctttgagg tccgtatccg ccggagcagt gacgatcgga tagagattcc ggccttgctt
    13081 ctcttcgagg ccgatcttgt cgactttcgc cgggccgctc gcgatatgtg cggctaccct
    13141 ggcggcgtgc atgatcttct ccgggctcag atcgtcgctg taggcatagc cggtgcgctc
    13201 acctgaaata acccggacac caacgccgag agacacaccc tggttcgcgc tcttgatgat
    13261 ggattcgtcg atgccgatcg agctggttgc gacatactcg aaatacaggt ccgcgtaatc
    13321 gccaccggcc gaaagcgcct cacccagata ccgctcgaga tcacgctccg tgatgccaaa
    13381 tttcccgccg aaaaaggaat cctgtaaccc catactttga gggtaacatc cgggagttct
    13441 ccggacgttt atgtgcgacc gatgctgctg cagccgtcag ggctattagc gctgccgcgc
    13501 gccgtgaagg atattgatga tctccagatt atctcagggt atcggtccag gcactcaatc
    13561 gccgctaaat tcgcgaagcc acggtagttg ctgcgtataa gctatccgct gcaatgtaat
    13621 cgtagatttc ctcgaggtcg gtggcggcgt tagaagccca aatgatcttt acttaggaga
    13681 cgggtttctt tggccggcca tcaggttctg caaccatggc ctcaatctct cgtagggaat
    13741 cccttctcct cgctccagtt gggcgataga ttcccggatg cgcgccgtct gatccagata
    13801 ggtctctatg gcctcttcag ccaggctggc aatcgattgt gaagtctcgc tggcgacctt
    13861 ttcgagccgt tccgaaagat gatcagggag cgcaatcgtc ttcacaggga ttgagaaaaa
    13921 gcgggccacg acagaaccaa aacgaagatt gcaaataaag actgattcat gcctgaaact
    13981 cctattagac atgatgtcag tctgtgactt gaacccgcta cctggacaaa ggcagaggtt
    14041 tcgggcgggc gccaccccgg gtgccgttcc agtagggctc gccttgcggc tggtaattac
    14101 ccagcttgtc ccagcgttcg cgtgttcgtc cgttgaggtc ccatacgaca aggccggaag
    14161 ccaccgtcaa ttcacacaac aggcgctgcg tgccgtccat gcgtgcgccc caggagtcga
    14221 cgaaagcgaa ctggcctttc tcgagccgca gcacggcgat atccgccagg gctcccaccg
    14281 agagatgacc gagctgctcc cggtgaatga tacgcgccgg gttccaggtg gagcggtaaa
    14341 taatgtcatc gagcgtcatc cccatattca agaatttgga catgacgttg agctggtcct
    14401 tcatgcccgc gttgatgctg ccggagtgaa tgtcggtgga gatgacatcg ggcggaaatc
    14461 cctgctttac ggcgggcacc gcctggcgga attcgaacgc ggcgccgccg tgtcccacgt
    14521 caaacagcac gccccgtttg cgcgcttcga acagataggg caacaggcgg ccattgtcat
    14581 cgagcatcgg aaccggcacg tagaaggcat gcgtgtagat gtcgccaggc cggagcttct
    14641 tcaacacaag ttcctggaac ggacgctccg ggcggaactg accgaagtcg atcatcaccg
    14701 gaatgttggc gagagtgccc gcttcgacgc cgcgctccac gggtgtccag tccgggccgc
    14761 ggtagtgggc gactttgatg ccgacgatgg tgtcgcggtt ggcgatggcc aggtttgccg
    14821 cggccttggc gtccatttcc tcgagatcct gttcctgtgc atcaccgggc atacccgcgc
    14881 ctccgatatt caggaaaacc agcacacggg ttcgggcgcg atcgatcacg gttctcttga
    14941 actgagcaaa gttgtgacgg ccggaagatc ccgcatcgac cacggtggta acccccgtgc
    15001 gaagcgcgaa gttatcgggg aagatgctgg cgtgcccgcc cgcgaggccc ttcccttcgg
    15061 agccggcgaa aacatgtgca tgcaggtcga tcaggccggg cgtgaggtaa agcccggcta
    15121 catccaccac tttgcgggcc tgcgccgccg gtatgttgcg ttcgacggcg gcgatcaggc
    15181 catcgcggat cgcgacatcg cgcacggcgc tgagcttgtt tttcgggtcg atgacgtggg
    15241 cgcccttcag gagaagatcg tagttggctt gggcgaacat tgcgttcgcg cacagcatcg
    15301 cggccaggat gcgcatagcg aactatctta acccacccct gaaggaaaaa ggagtcattt
    15361 tctgcgaaaa aacgtctttt cgcgcttata atggaaagcc atgaaatcca cgcgccgccg
    15421 ccttttgcag actgccgcgc tgtcgcccgc ggcagcagcg gccgccgcca ccgggaatct
    15481 cactgtctcc tcgccatcgc cgttatatgc aagcctgggc attaagcctg tcatcaacgg
    15541 cgtcggggtg gtaaccgtcc tcggcggttc gattatgccg cccgaagtga tccgcgccat
    15601 ggaggaagca tcacgttact tcattccgct gcccgagctt caaagaaaag tgggaatgcg
    15661 catcgcggag ctgttgaagg ctcccgcggc gatggtcacc tgtggcgcgg cctcggccat
    15721 cacggtcggt accgccgcct gcctgtccga aggcgactcc gctaagctcc ggaggctacc
    15781 caatcgcgag ggcatccgct acgaagtgat tcagcagaag tcgcaccgct ccggctatga
    15841 gcatcagatg gagttgtgcg gcgcgaagat cgtcaccgtg gaaacacgga aagacctgga
    15901 agccgccatt aacgaaaaga ccggcatgat gttctttctc aacaaggcgg accccgacgg
    15961 ccaggtcaag gccgaagagt tcgtgaagat cggcaaggag cggggcgtgc cgacgatgaa
    16021 cgatgccgct tccgatgcca cacccaaaga aaacctgtgg aagtacacgg aacaagggtt
    16081 tgatctcgtg atattctccg gcggcaaagc tcttcgaggc ccgcaagcca gcgggttgct
    16141 tctgggaaga aaggacctga tcgaagcttc ctaccccgcc atgtcgccct acggcggaat
    16201 tggccgcggc atgaaggtgg gcaaagagga gatgtgcggt ctgctggccg cggtggaacg
    16261 ttatttgaag gtggatcacg aggccgagta ccgggaactg gatcggcgtg tcgcgtcgat
    16321 tcgccaggcg ctccagggtg tctccggtgt tcagacggaa cgccatgtgc cggtcattgc
    16381 caatgaggtg ccgcacgtca aggtcctgtg ggatgaatcc gcacggggct tgtcatcaca
    16441 aggggtggtt gaaaagctgc gcgacgggga cccgccgatt cacatccagc gccccggcaa
    16501 cgggcaactc ctgatctccg tctggatgat gcgcggcacc gagcacgaga tcgtcggccg
    16561 gcgattgaag gaaatctttt cgacgtaggc gaaaacggaa gagcgggcct aaaggcggac
    16621 cagccccagg ctgatctcaa tggcccggga agtctacttc gtacgctttc tggtgggagt
    16681 ggcaggtttc catgcttcct catcaaggga aaaccactcc cgggcaatct ccagatcccg
    16741 ctgggcgttg gtgatggcgc cttctttcag acgctcggtg agggtgtttt tggccttgct
    16801 gttgacatcg tacataatgg cttcggaaat gaactggctg cggttatcgt tggtggcagc
    16861 ccggttcaga acttttcgtc ctgatgggag cgcttccttc agcgctccca tcaaacgcgt
    16921 gtctcaaaat tacttctggg tagcgggcct gcccggatga cccggaatga gccggtaatc
    16981 cgggggaatc tggaaccatt cggccggtgg ctcggtgtcc atctgcatgt tttccagttc
    17041 cgagaccata cgcaggttct cgcctccaac agtcactttg atatcttcgt ataccaacag
    17101 aattcccaac tcaggggaca cacacgttgt tccgatcact tcgctctcgc cgtccggttt
    17161 gacgcgcttt gtaggctcct gcagacaccg gacgccattg atctccacgg actgcatttg
    17221 cgacttcgag aggtccggtc tagaagcggt ggttagtaaa ataagcaacc ttctgctgcc
    17281 ggttcacctc gtacgaccgc cctgagacaa agtcccggac ccagtcatag cggaatcgct
    17341 ccgggacaat cgaggctccc gacgaatacc gccgtccctg gctgtcgcgc gcttcggttt
    17401 ttcggacgat atctttcaaa acctctggtt tacccggctc ggcaatgtag atggttcttt
    17461 tggaaaccgc gatgtagggg gggtggcgtg acggttcggt ttgtgaagag agtacggccg
    17521 gcatgaggaa cgcccagcag ccgagaacta tcgagttaca aaagcgatat ctcatggtat
    17581 ctgctcctta tcgctatcgt ctttagccgt taggcatagc aaaaacagcc ttgacagtaa
    17641 tggtagccga atggatacgt gttgcaagac tgccaatagg ccccaaccgc ggcagcggag
    17701 gtgcacagat tctcctcctc cgctgccgtc aaactggtgc cgaaatttgt gccaaagctg
    17761 aagtgaaaat cccattgctg ccctcctttg ggaggtcggt tatactgcca tgcgttactg
    17821 actgcaatat ggaaaaaaac aacaccatgc gcggaagcgg taacggcgga tacaccactt
    17881 gtccgttcgt gaccgggtac gggaaagtgc tcatggccga gttcggctat gacaagaaac
    17941 cagccctaca atacctttcc tcgaccccgt ccatgaacat cggaccagat ggatcttgaa
    18001 gcggtatgtc ctgaagccca tgtactttga gcttatgctc cgcggacggg catagcctcc
    18061 gggactgcgg ctgcaggtat acgaaggcgg ttggagatac tccgaacagt caattcatag
    18121 gagcaagctt gcccgcatac cctgtaatga gctggaaatc cggtggaatc tgggaccact
    18181 cggacggcgg ctccgtgtcc atttgcatat tttccagttc cgagacgata tgcaggcgct
    18241 caccctgcac gtctatcttg tggtcctgat gcactgtcag gttccctaac tcagggaaca
    18301 cgcacgttgt tcccatcact tcgctcccgc caccttgttt gaccatcctg acaggtccct
    18361 gcacacaccg gatccgttga tctctgcggt ccgcatttcc gggtgcgaga ggtcaggtcc
    18421 ggaaccggaa gggtcgaggt gggtgaagta agcaatcttt tgctgcctgc aatatggaag
    18481 aaaacaacac catgcgcggg agcagcaatg acagtggatt cagacgtttc gaactacttc
    18541 tcaatggaag ccgcggcgat ttgcgcgatg tccaggagtt gtggagatgc ctccatggac
    18601 gacaatgcgt cgcggaacat ggtgttgcag aacgggcagg ctgccccgat gacgtccgct
    18661 ttggtcgcca cgagttcctc tgcacgctca acattgacgc gcttgccctt ttcctctccg
    18721 agaaacgcca gcccgccgcc tgcgccgcaa cagaacgaac ggtgccggct tcgcggaggg
    18781 tcgatcacgt gtccacccag gcgggcgatg gatcgtggct cctcgtaaat gtcgcgatag
    18841 cgcccgagat agcagggatc gtgaaaaacg atttgcctgc tgccgccgtt cgaaggcagg
    18901 cgcgagcgat tccggaacaa tagctcgctg tgatgttcaa tggggaaaga tgcgccgtat
    18961 tccttccagt cctgctgtat ggtttcgacg caatgcgggc atatggaaac aagcctcttc
    19021 actccgtgct cgcgcatctg ttccaggttg aattccgcga gttcaccgaa cagcaggtcg
    19081 ttgccgagcc tgcgtgccgg atcgcccgag cacttctcgc gtttcaggac accgtaggtg
    19141 atacccagat gctccagaac ttttacgagc gactgcacaa tctcccgccc gcggggatcg
    19201 tatgcgccca tgcagcccag ccagaggcag aactcctgcg atccgtcgaa cagcggcagc
    19261 ccggccttct ggatgaactt gtcgcgctcg gaggcagaca ttcccagcgc attgccgttc
    19321 ctttccaggt tcagaaagaa cctggtgcca tgttcgtctt cccacctgcc cgtatttacc
    19381 gcgccccggc gcaggcccac gatcatggga agatgctcga cgccgaccgg acactggtat
    19441 tcgcacgctc cgcaactggt gcattggaaa accgcttcca tggaaacgtg gttgtcgaga
    19501 accggctctt ccccagcagt tccggggccg cggagatagc tgcgcaggcc cagtaccacc
    19561 tgcttgggat tcaggatctt tcccgtgtta ttcgccggac aatgttccgt acagcgcccg
    19621 cactcgacgc acgaaaacgc ctgaagggag tcgatacgag tgaaatcctt gcccataaac
    19681 aggccgaagt cctcgtcgcc ctctaaaggt ggtatgcggc tgaacgatcc tctccgtaga
    19741 aagatcgtga aaggactaag taccaggtgg aggtgcttgg tatggggaat caagggaagg
    19801 aataccagga gcgccagcgt gtgcgcccac cagttggcac gctggaacgc gctgtcttcg
    19861 tgtgcccacc actccgcgag gtaggtaacc atcaggaaga aaataagagc ggcaatgatg
    19921 ccggattcgc ccgataactc gccgagccat ttcggccgca caaggaaccg ccgcaccgcc
    19981 agtccggcaa tgccgacgga aaccgcgacg gcgaatgccg ctgcccaacc gaaataaagc
    20041 cgtccgaagc tgctttcccg ggagatcaga tcgaatccga atccggacgc gatatgatcc
    20101 aacgtaacca gcgcgaatac acagaaaccc cagaacaccc atgcatgtgc aattcccgcc
    20161 atcggtcttt cacggatgac cttggcctgc agcatcactt ccccgatgaa atccagaacc
    20221 cgccggctca ccggcctgat ctcgaatcca ggcgccggct tcgacgcgcg tatgattcga
    20281 aggacccttc caaaccggcg ccagaacagc aataccgaca tcgctataag gagtgctagt
    20341 accaacatct ccccggccga gggcgtgttc atcttgctaa agactgtaac atgaggagca
    20401 ttcggtacaa tgttgtactt tggggggaat cagcgagtgc gcttcgtcat ttccggagca
    20461 gccggattca tcgggtctca tctctgcgac agattgatcg cggaagggca ctccgttctg
    20521 gccttggaca acttcattac cggttcgccc gctaaccttg cgcacctcga aggcgcggac
    20581 ggcttcgaat tccgggaaca cgatgtcacg agtcccattg aggcgtccgc cccggtggac
    20641 ttcgttctgc atcttgcttc tctcgcgagc cccaaggact atctgaacca cccgattgaa
    20701 acgctcgaaa cgggatccac agcgacgcgc aacatgctcg agctggcccg cgcccatcgc
    20761 gcacggttcc tgctgacatc gacgtcggag tgttatgggg acccgctcga gcatcctcaa
    20821 gcggaatctt actggggaaa cgtcaatccg gttggaatac ggtcctgcta tgacgaaagc
    20881 aagcggtacg cggaagcgct cacgatggcc tatcatcgcg tacacggttt gcgaacgaat
    20941 atcgcgagaa tcttcaacac gtacgggccg cggatgcagt tgaacgacgg acgtgtggta
    21001 cccgcctttg tcgaccaggc gctacgcggc gaagctttga cggtgttcgg cgacggcaag
    21061 cagacgcgca gcttttgcta cgtcagcgat cttgtggaag gcctttaccg gctaatgttc
    21121 tccgacgagc gttaccccgt gaacctgggc aatccggatg agatgacgat tctcgaattt
    21181 gccgagcatg tccggaagat caccgccgcc gccgccccaa tccggttcga accgcttccg
    21241 tccgacgacc cgcagcgccg ccgtcccgat atcaccaaag cgcgcacact gctggactgg
    21301 gaaccccgag tcagcctgga ggacggactc cgcgagacga tcgcctattt ccgcggcttg
    21361 tacgcctctt ctgttcctgt gcgttctctc gagtagacgc atcgcgggga tacgtcgcaa
    21421 gataattacg ccatttttgt gccgccgcct tatcgaaacg ctcctgccag aaccgccgaa
    21481 actcctcgat cctccgggcg ctccgcgcct ccagccatgc gacgaattcc ggggaatcgc
    21541 tctccgctaa catcgctctt gtggtggagc agtcgctgat cgctcccaac tgatcctgca
    21601 gctctttcat caatgaggcc agttgcgcga ggccgggacc gtgtacgcca cggaacatct
    21661 ccagtgaata gcgaaacttt tttgccgcca gccggaagtc gtgcaatgct tgcggatcac
    21721 cgcttttgag taccggttcc gcccgctcat agaacgcgct gaagaactcg ggaagcacgg
    21781 tgcgggcgtt gacggccacc gaagacgatt cactccactt gaacttcttc atgcctccag
    21841 cctcaacgac tcgcgccact tgacggaata attgcggcgg ttcaggcgcc tcaacgcggc
    21901 cgcaagttcc ttcatggaca cctttcgttc cagttccatc cgcttgcgca ccccggtacg
    21961 gtccggcgcc tccgactctt ccagcagatg aagcgcgata tcgcggctgc gcacctccgc
    22021 cgagagattc ataatcgatt tcagccgttt acggatccgt ttggatggct cgccggggat
    22081 gaactgccgg aagaagcgta gcgcctgcac gaagcgacgg atcgagaccc tggcctcgtg
    22141 aacacgtttc tcatcggggc ttttggcggc gcggcttaca tggtaaacga cccgatccag
    22201 caaactggag atctgggaaa gggtatagac gcgaatgttt tcggctccgg aggatttcgt
    22261 catgtctgtt tgcgagccct cgtaatggtg agctgtagcg cgaatacctg ttcgaagagt
    22321 tcagccaccc gctccgccgc ccacatctcg agatccgtat ccactgccga ttcgaccttt
    22381 acggtcacgc cgccattgcg catctggcag gcgatcccct cgacgcgctg gtcgtggctt
    22441 cgatcgagcc cgtcggccag ccggagcatc ggtgtcaggc gcatgaccgt ctgccgctcc
    22501 tcgggagtta acgcctgaaa tatatcgtgc cgcggcgcgg gcagcgattt tcgatggtag
    22561 cggcacaaca gggcggtcat cttccgttcc caggcggtaa agcccggcat atcggagttg
    22621 gaaacaagat aatgagaatg cttatggtgg ccgacatcgc ttacaaaatg cccgacatca
    22681 tgcaggtaag cggccgcttc cagcagtctt ccggtctcga gcgggagcct gtggacgggc
    22741 gccagcgaat cgaacaggct tcttcccagg gcggccactt tgcgcgcgtg cttcacgggg
    22801 acaccatagc gtctcgccat ctcttccacc gaccttcgct gttccggtgt cagttgggcg
    22861 cgctctcttc caacacctcg cgcggccagg tcggcaatga taccgtctct gagtcccgcg
    22921 gacgaatagt agatggatgg cagccgtagc gcctcgagga ttttcagaaa taccgccgcg
    22981 ccgggaacga taatctcggc acggcgtgga cctatgcctc tgaccttctt ccgcgccgcc
    23041 aggtccagac cgctgatctc ccgatagaac ttccgcacct gcggcgttgt ggctcgcaac
    23101 ttgtcggccg cctcccgctt cgaccgcgct acccggttga tggagcctac taccgccgcg
    23161 gcggatgcgg acgtggcaat gacccgatcc atccggcgcc tgccgatacg cgccaccgcc
    23221 gctccaatct tctcctgtat atattcttcg agctgatgaa gttctttcgc tgtgggcggg
    23281 tcgtgtgtaa ggaagaccga agtcagacga accgcaccca ggggcttcga gaacgcctcc
    23341 acgatgcggc cgttctcgga aacgatgagc tcagcgcttc ctccgccgac atcgattacc
    23401 aacgtccgcc tgtttttgtg cggccagcgg ctttcgacgc ccagttggat gaggcgggct
    23461 tcctcctggc ctgagatcac ttccactgcc gctcccgccg cttcggaagc gcgctccaaa
    23521 aactcccttt ggttgctggc gtcgcggacg gcgctggtgg cgaccgcgcg aatgcctgcg
    23581 acatccaact ggcggtagat tgccgccatt tttcttaatt gggagcaggt gaattcaagc
    23641 gcttccggac tgatccggcc cgtacggaaa acgctctctc ccaggcgtgt cacatccctt
    23701 tcggctgcca gcacccggac cggagagccg gggattactt ccgcggcctg catccggacc
    23761 gaattgctgc cgatgtctac ggccgcgtac ttcggcatat accgttataa tagtcatcct
    23821 gaccttcctc ttatgccctt attcgcagcg aaccgttaca aaggcaagct gatcgttgtc
    23881 gaaggcatag acggctccgg taaatccacc cagatttcgc tactcagcca atggctgcgc
    23941 tccaatggca ttgccgtggc ttttagcgaa tggaattcat ctccgctggt ccgcgaaaca
    24001 acaaagcgcg gcaagaagaa agagatgttt acaccgagca cgttcagcct gatccacgcg
    24061 actgactttg ccgaccgtat ggagcggtat atcatcccgt tgatgaaggc gggcgccgtg
    24121 gtctgtgcgg accgctacgc ctatacggcg tttggccgtg atgtcgtacg gggcgtcagc
    24181 cgtaattggg tacgcaacct gtatcgcttc gccgtgcgtc cgaacgtggc attctatttc
    24241 cgcgtgcctc tcgacgtggc tctgggccgc attctgggcg gcagggatgc catcaagtac
    24301 tacgaagccg gtatggacct gggactcagc cggaacgtgg aagaaagttt ccgcattttt
    24361 cagggccgga tcatcg
//
LOCUS       DASTMK010000333         6880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_367913, whole genome shotgun sequence.
ACCESSION   DASTMK010000333 DASTMK010000000
VERSION     DASTMK010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367913"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="VFQ79_14565"
     CDS             complement(<1..1906)
                     /locus_tag="VFQ79_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU0140943.1"
                     /translation="MKKDLLPTESLDSRELLKFVTAFRKGDFSARLPADRSGTCGKIY
                     DTLNDIIELNDRMCREFDRISRTVGKEGKTSARVSVGSANGSWVACIEAVNGLIGDLV
                     QPSTEVARVIGAVAKGDLSQTMPLEADGRSLKGEFLRTARVVNTMVEQLSSVTSEVTR
                     VAREVGSEGKLGGQADVKRVGGAWKDLTDSVNSMAGNLTAQVRNIAEVTTAVAKGDLS
                     KKITVSVRGEILELKDTINIMVDQLNSFASEVTRVAREVGTDGKLGGQAMVKGIAGTW
                     KDLTESVNSMAGNLMAQVRNIAEVTTAVARGDLSKKITVDVQGEILELKNTINTMVDQ
                     LGSFASEVTRVAREVGTEGKLGGQARVPDVGGTWKDLTESVNSMAGNLTAQVRNIAEV
                     TTAVARGDLSKKITVDVQGEILELKNTINTMVDQLGSFASEVTRVAREVGTEGKLGGQ
                     AEVPDVGGTWKDLTESVNSMAGNLTAQVRNIADVTTAVANGDLRRKITVDVRGEILEL
                     KNTINTMVDQLNGFASEVTRVAREVGTEGKLGGQAQVKGVAGTWKDLTDSVNSMAGNL
                     TDQVRNIAQVTTAVARGDLSLKITVDVRGEMLRMKDTINTMVDQLNGFASEVTRVARE
                     VGTEGKLGGQA"
     gene            complement(2039..5449)
                     /locus_tag="VFQ79_14570"
     CDS             complement(2039..5449)
                     /locus_tag="VFQ79_14570"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0140944.1"
                     /translation="MRYLFILWAVVSLSLRTMPAQELRASVSGTVMDPSASPIPNAKV
                     SVRNLDTNVVTDTVTNEVGRYTLLFLQPGRYALSVEASGFKRYSNENVVLGTSDRVSL
                     DVPLEVGQITESVTVTAESTLLQTETASAGSLVTTSQVRDIPNNGRNVYQLVWAAPGV
                     IKNSRYWGSMENYALGNSTGVSINGGIRRENETVMDGVTNTQPNRDVNFQPPLESVAE
                     MKVQVSNYDASYGRFGGGVVAITTKSGTNSLHGSLYEFNKVAALAANPWVYNFYGEPK
                     PHFVNNTFGFQVDGPIYLPRIFDGRNRLFFMVAYEGLRERSSGGDATVLPTQGMRSGD
                     FSGLPVTIYDPLTTRSEGGRFVRDPFPGNRIPANRISPVAQRVLEFVPQPNLTGRGYG
                     LENYANFGGAKNGYDQFLTKLDYRVNDRNNVYFSYGRLPYQEFDDILFGGDSPAEPSR
                     ENPLHRNFYRYVFDWTSTLSPQTVLNFRAGLARYVDIGGSPPAVGFDPRNLGFADSLV
                     SQFTFLAFPRFNIGGFYTSVGSDRALNQSVRDSYSYQLNLNRSQGRHQLKYGAEFRIY
                     NESAQSPGNASGTYQFSKGFTQANPAQADRESGDEFAAFLLGYPSSGSVDMNIDPYFK
                     NHYFALFVHDDFKIHPRVTLNLGLRWDYEQPPAERYNRMLRGFAFDQPSPIANQVTGL
                     DLRGGLLYAGSDGDARRAFNSDKNNIQPRVGIAVQLTPKTVLRGGYGLYYLGASGGQP
                     TTGFSQTTPLVSSADAGNTPRVSLVNAFPDGLIRPQGSSQGMSTLLGQGISFSYLDRV
                     LPYAHQYSVGVQRSLPGGFVVDASYSGNETRRYPVTANLNAIPTDQLGQPDTYYREQV
                     PNPMRGLLPLNASKNGATIVRQDLLVPFPQYSGVTMSNVPIGRNHYHSFQVSARKRYS
                     QGLTMNLSYTISKTIEELTFLNAQDFNLQDIDASRLERRLTEYDVPQKFAALVNYELP
                     FGRGKPFGAGATGVAGKLISGWQFSTQTTFQSGFPVDFPNAPNLEARSAKLPSDQISL
                     FNAFDKTLFPSTTPNLSYQLRTWPTRFPDVRLYPLKNVDFSVSKRTPIGERMNVELRA
                     DFLNAANHPWFSRLHNNGTDVTRPEFGWYFLEEQNQNRLIALVLKFSW"
     gene            complement(5560..6408)
                     /locus_tag="VFQ79_14575"
     CDS             complement(5560..6408)
                     /locus_tag="VFQ79_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0140945.1"
                     /translation="MKFGVNMFIWTSEFGESDLPLLPTIKERGFDGIEIPIFSPSTFP
                     AARIRQALRENGLETTICSVIPNGLSIIADDPNVRAQTIAHLEASIAGAAEVGARVFA
                     GPLYSPVGYLPGRRRTPDEWKRAVEGFQTLGPALAQHDVTIAIEPLNRFETYFLNTAT
                     DAVALVREIGHPNVGLLVDTFHANIEEKSIADAYRTAGPYLKHVHTCENDRGTPGSGH
                     VEWQDVFQALREVKYDGWLTIESFGFAIGGLSAAASIWRDLARTPADIAFEGLAFLKQ
                     NTVKFA"
     gene            6513..>6880
                     /locus_tag="VFQ79_14580"
     CDS             6513..>6880
                     /locus_tag="VFQ79_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0140946.1"
                     /translation="MASDHAIETEDLTKVFRNRWTGREIRAVDGVSLQIRRGTTFGLL
                     GPNGAGKTTFIKMLLSATHPSSGAARIFGGDARLASSRKPVGYLPENHRFPTYFTGAG
                     MLDFYGALSGMEAAERRRRST"
BASE COUNT         1349 a         2042 c         2005 g         1484 t
ORIGIN      
        1 gcgcctgccc gcccagtttt ccttccgtgc cgacttcgcg cgccacgcga gttacttcag
       61 aggcgaagcc gttgagctgg tccaccatcg tgttgatggt gtccttcatc cttagcatct
      121 cgccgcgcac atccaccgtg atcttgaggg aaagatcgcc gcgcgccacg gcggtggtta
      181 cctgggcgat gttgcgaacc tggtcggtga ggttgccggc catcgagttc acgctgtccg
      241 tcagatcttt ccaggtaccg gcgaccccct tcacctgcgc ctggcctccg agttttcctt
      301 ccgtgccgac ttcgcgcgcc acgcgagtta cttcagaggc gaagccgttg agctggtcca
      361 ccatggtgtt gatggtgttc ttgagttcga ggatttcgcc gcgcacgtcg accgtgatct
      421 tccggcgcaa atctccgttc gccacggccg tggtgacgtc ggcgatgttg cgaacctggg
      481 ccgtgaggtt gccggccatg gaattgacgc tctccgtcaa gtccttccag gtgccgccca
      541 cgtccggcac ctccgcctgg ccgccgagct tgccttcggt gcccacctcg cgcgccacgc
      601 gcgttacttc ggaggcgaag gaaccaagct ggtccaccat ggtgttgatg gtatttttga
      661 gttcaaggat ttcgccctgt acatccaccg tgatcttttt cgaaagatcg cccctggcga
      721 cggcggtggt gacttccgca atgttgcgga cctgcgccgt caggttgccc gccatggagt
      781 tcacgctttc ggtcaaatcc ttccaggtgc cgcctacgtc aggcaccctg gcttgcccgc
      841 ccagcttgcc ctcggttccc acttcgcgcg ctactcgtgt cacttcggaa gcgaaagacc
      901 caagctgatc caccatggtg ttgatggtgt tcttgagttc aaggatttca ccctgtacgt
      961 ccacggtgat ctttttggaa agatcgcccc tggcgacggc ggtggtgact tcagcaatat
     1021 tgcggacctg cgccatcagg ttgcccgcca tggagttcac gctttcggtc aaatccttcc
     1081 aggtaccggc aattccctta accatggcct ggccgccgag tttgccgtcg gtacccactt
     1141 cgcgcgccac ccgcgttact tcggaagcga acgagttaag ctggtccacc atgatgttga
     1201 tggtgtcttt gagctccagg atttcgccgc gaacgctaac ggtgattttc ttggaaagat
     1261 cgcccttggc gaccgccgtg gtcacttccg cgatgttgcg aacctgcgcc gtcaggttgc
     1321 cggccatgga atttacgctg tcggtcaggt ccttccacgc tccgccgacc cgcttgacgt
     1381 ccgcctgacc gcccagtttg ccctccgatc cgacctcgcg cgcaacgcgg gtcacctcgg
     1441 aagtcaccga acttaactgc tctaccatgg tgtttactac gcgtgcggta cgcaggaact
     1501 cgcctttcag cgaccggcca tccgcttcga gcggcatcgt ttgcgacaaa tcgcccttgg
     1561 cgacggcccc aatgacgcgc gccacttccg tcgacggctg taccagatcg ccgatcaacc
     1621 cgttcacggc ttcgatgcag gcgacccagc ttccgttggc ggacccgacc gatacgcggg
     1681 ccgaggtttt tccctctttg cccacggtgc ggcttatcct gtcgaattcg cggcacatcc
     1741 ggtcgttcaa ctcaatgatg tcgtttagcg tgtcatagat cttgccgcag gttcccgatc
     1801 ggtcggccgg gagccgggcg gaaaaatcgc ccttgcggaa ggccgtgacg aatttaagaa
     1861 gttctctgga atccaggctt tcggtgggga ggaggtcttt cttcatcgtt ggtgcggcgg
     1921 gaaagtggag cctgaatggg cacaccccca cgcggattcg acgccagtag cggaaaaggg
     1981 tttcatcgca ggcaccggcc ggctgacggg cgcctgcgag agatctcgga gattgggact
     2041 accagctaaa cttgagaacc agggcgatca ggcgattttg gttctgttcc tcaaggaaat
     2101 accagccgaa ctccggccgc gtcacatcgg tcccattgtt gtgcaggcgc gagaaccacg
     2161 gatgattggc ggcattgaga aaatcggccc gcagctcgac gttcattctc tcgccgatgg
     2221 gcgtcctctt cgagacgctg aaatccacgt tcttcagagg atagaggcgc acgtcgggaa
     2281 agcgcgtggg ccaggttcgc aactggtacg aaagatttgg cgtggtggaa gggaacagcg
     2341 ttttgtcgaa tgcgttgaag aggctaatct ggtccgacgg aagcttcgcg cttcgggcct
     2401 ccaggttcgg ggcattcgga aaatcgacag ggaaaccgga ctggaacgtt gtttgcgtac
     2461 tgaactgcca gccggagatg agtttgcccg caacgccggt agctcccgcg ccgaacggct
     2521 taccccggcc gaacggcagt tcgtaattca caagcgctgc gaacttttgt ggaacgtcgt
     2581 attccgtcag ccgccgctcg agtctggacg catcgatgtc ctgaagattg aaatcctgcg
     2641 cattcagaaa tgtaagctcc tcaatggttt tcgatatcgt gtacgacagg ttcatcgtca
     2701 gcccctgcga gtagcgcttt cgcgcggaca cctggaacga atgatagtgg ttccgcccga
     2761 tcggcacatt gctcatggtg acgccggaat actgcggaaa cggaaccagc agatcctggc
     2821 ggacgatggt agcgccattc ttcgacgcgt tcagcggcag cagtcctcgc attgggttcg
     2881 gaacctgttc ccggtagtaa gtatccggct ggcctagctg atccgtggga atggcgttca
     2941 gattcgccgt gacagggtag cgccgggtct cgttgccgct atagcttgca tccacgacaa
     3001 atccacctgg caggcttctc tgcacgccga cggaatactg gtgcgcatac ggcagcacac
     3061 ggtcaagata actgaacgaa atcccttgcc ccaacaatgt cgacatgccc tgcgaactgc
     3121 cctgcgggcg aatcagcccg tcgggaaacg cgttcaccag agagacacgg ggcgtgttcc
     3181 ccgcatccgc gcttgaaacc agtggagtcg tctggctaaa cccggtcgta ggctgccctc
     3241 cgctcgcccc gaggtagtac agtccgtagc cgccgcgcag cactgttttc ggtgtgagtt
     3301 gcactgcaat accgacacgg ggctgtatgt tgttcttgtc cgaattgaaa gcccggcgcg
     3361 catcgccgtc cgagccggcg taaagcaacc cgccgcggag atccaggccg gtaacctggt
     3421 tcgcgatagg gctaggctgg tcgaaggcga acccgcgcag catgcggttg tagcgctcgg
     3481 ccggaggctg ctcgtaatcc caccgcagac caagattcag cgtcacgcgc ggatgaatct
     3541 tgaaatcgtc atgaacgaac agcgcgaaat agtggttctt aaagtacggg tcgatattca
     3601 tgtctaccga accgctggag ggatacccga gaaggaaagc ggcgaactcg tcgcccgatt
     3661 cccgatccgc ttgcgccgga ttggcttgcg tgaatccctt gctgaactga tacgtgccgc
     3721 ttgcgttgcc cggactttga gcgctctcgt tgtagatgcg gaactcggcg ccgtacttca
     3781 gttgatgtct gccctggctg cggttgagat tcaactggta cgaatacgag tcccgaaccg
     3841 actggttcag cgcgcgatca cttccaacag acgtatagaa gcctccgatg ttgaatcgcg
     3901 ggaacgcaag aaaggtgaac tgggaaacca ggctgtctgc aaacccgagg tttcgcgggt
     3961 cgaatcccac cgccggcggg ctcccgccga tgtcgacata gcgagctaga ccggcgcgga
     4021 agttcaatac tgtctgcggg cttaatgtgc tggtccaatc gaacacgtaa cggtagaagt
     4081 tccggtgcag agggttttca cggctgggct ccgccgggct gtcgcctcca aacaggatgt
     4141 catcgaattc ctggtatgga aggcgtccgt agctgaaata cacattgttc cggtcattga
     4201 cccggtagtc gagcttcgtc agaaactggt catatccgtt cttggcgccg ccgaagttcg
     4261 catagttctc cagcccgtaa ccgcgtccgg tcaggttcgg ctgcggaacg aattcgagca
     4321 ctctctgcgc caccggactg attcgattcg ccgggatacg atttcccggg aacgggtcgc
     4381 ggacgaagcg gcctccttcg gaacgagtgg tcagcgggtc gtatatcgtg acaggcaacc
     4441 cggagaaatc gcccgaacgc atcccctgag taggaaggac cgtcgcgtca cctcctgatg
     4501 aacgctcccg caatccttca tacgccacca tgaagaacag ccggttgcgg ccgtcgaaaa
     4561 tcctggggag gtatatcggc ccatccacct gaaaaccgaa ggtgttattc acaaagtgag
     4621 gtttgggttc gccgtagaaa ttgtaaaccc acggattagc ggccaaagcg gccactttgt
     4681 tgaattcgta aagcgagccg tgaaggctat ttgtccctga tttggttgta atcgctacga
     4741 cgccaccgcc gaatcgtcca tagctggcgt cgtaattgct tacctggact ttcatttccg
     4801 ccacggattc gagcggtggt tgaaagttca catcgcggtt cggttgtgtg ttggtgacgc
     4861 cgtccattac ggtttcgttc tcgcggcgga tgccgccatt gatcgaaaca ccggtggagt
     4921 ttccaagagc gtagttctcc atcgaacccc agtaacggct gttcttgatc actcccggcg
     4981 ccgcccacac aagctgatag acatttctgc cgttgttcgg aatgtcccga acctggctcg
     5041 tcgtcacaag gctgccggcg gatgccgttt cggtctgcaa tagtgtcgac tcggcggtta
     5101 ccgttacgct ttcggtgatc tgcccgactt cgagtgggac gtcaagcgac accctgtcgg
     5161 aagtcccgag cacaacgttt tcattgctgt aacgtttgaa ccccgacgct tccacggaca
     5221 gagcatagcg gcccggttgg agaaacagca gtgtgtatcg tcccacctca ttcgtcacgg
     5281 tgtcggtgac cacgttcgta tccagattgc gcacagatac cttcgcgtta ggaatgggag
     5341 acgcgctcgg atccatcact gtgccggaca cggatgcgcg cagttcctga gcgggcatgg
     5401 tccgtaatga cagagacacc acggcccaca aaataaacaa ataccgcatg cttattttca
     5461 cgacttaccc cctgctgagt aggagaatag gtcagtattc cggcactgtc aagggaaatt
     5521 ttgtttcagc gtttcatgtt tttaacaaaa cgcctacaat tatgcgaatt taacggtatt
     5581 ttgtttcagg aatgcgagcc cctcgaacgc gatgtcggcc ggagtccggg caaggtcccg
     5641 ccaaatggaa gcggcggcag ataggccgcc gatcgcgaat ccgaagctct cgatggtgag
     5701 ccagccgtcg tactttactt cccgcaaggc ctgaaacaca tcctgccact cgacgtgccc
     5761 cgagccgggc gtaccgcggt cgttctcgca cgtatgaacg tgcttcagat aaggacccgc
     5821 ggtgcggtat gcatccgcga tgcttttctc ctcgatgttg gcgtggaacg tgtccaccag
     5881 cagaccgacg ttcgggtggc cgatctcgcg caccagggca accgcgtcgg tggcggtatt
     5941 caggaagtag gtctcgaacc ggttcaaagg ctcaatagca atggtgacat catgctgtgc
     6001 cagggcaggc cctagagtct gaaagccttc cacagcgcgc ttccactcgt ccggcgtgcg
     6061 gcggcggccg ggcaggtatc ccaccggaga atatagcggc ccggcgaaca cgcgcgcgcc
     6121 cacctctgcc gcaccggcga tcgaagcctc aagatgcgca atcgtttgag cacgtacgtt
     6181 cgggtcatcc gcaatgatgc tgaggccgtt cggaataacg gagcagatcg ttgtttcgag
     6241 accattctcc cgaagcgcct ggcgaattcg ggccgccgga aacgtagagg gactaaatat
     6301 cgggatttcg attccgtcaa aaccgcgttc cttgatcgtg ggcagaagag gaaggtcgct
     6361 ctcaccgaac tccgaggtcc agatgaacat attcacaccg aatttcattg aacaagctcc
     6421 cgttgtatcg cgttgataag tagaaggaca gcgcgtgcat tgtatcaaag tgagcctcgc
     6481 gcgcggcgat ggataatgga atcaactcct gcatggcatc cgatcacgcg attgaaacgg
     6541 aagacctgac caaggtattc cggaaccgct ggaccggccg cgagatccgg gcggtggacg
     6601 gtgtctcact ccagatccgg cggggaacca cgttcgggct tctcggcccg aacggtgcgg
     6661 gcaagaccac cttcatcaag atgctgttgt ccgccacgca tccgagctcg ggggccgcgc
     6721 gcatcttcgg cggcgatgcc cggctggcct cctcgcggaa accggtcggc taccttcccg
     6781 agaaccatcg ctttcccacc tatttcaccg gggccggaat gctggatttt tacggcgctc
     6841 tctccggaat ggaagcggcg gaacgccggc ggcgctcgac
//
LOCUS       DASTMK010000334         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_432185, whole genome shotgun sequence.
ACCESSION   DASTMK010000334 DASTMK010000000
VERSION     DASTMK010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432185"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFQ79_14585"
     CDS             <1..257
                     /locus_tag="VFQ79_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140947.1"
                     /translation="AISELQRLRPCPAGDKGIAFFDPRWEDGEHGLTMIARLVCRNPT
                     LKADLVNESGMVADRTRYAYTITYDAAKNSYIVHGSDRLH"
     gene            264..1214
                     /locus_tag="VFQ79_14590"
     CDS             264..1214
                     /locus_tag="VFQ79_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0140948.1"
                     /translation="MAILGIDIGGTRVKAGLVSGAGEVLRRLSVPTPSSLDAFTIALR
                     ELIANLLGDAGQPEAIGFGCKGIIDFHTARIEVLPGTLHFLEGSNLADLAGLSVPVYA
                     DNDARAALAGEVAWGAGAGCRNAVMFTLGTGVGGGVLAEGRILRGEKGVAGHLGHITV
                     DPDGPVCICGNHGCLEAVFSASAIESEAIGAAYRGCASILTRLYRQDPASLTCQAVFQ
                     AAAEGDSVALAIRDRAVKYLGASIAGLIHVFDPEKVILGGLISEAGDALFGPVREHVR
                     WRTRVLLGREVPVVKAQVSDSVVGAAAVALLGRSLAAPRA"
     gene            complement(1222..2643)
                     /locus_tag="VFQ79_14595"
     CDS             complement(1222..2643)
                     /locus_tag="VFQ79_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEU0140949.1"
                     /translation="MGKVTGFLEYTRELPQRRPVTERVNDWFEIYLDFPLDRVQTQGA
                     RCMDCGVPFCHTGCPLNNLIPDFNDLAYRDRWKDAIRTLHSTNNFPEFTGRVCPAPCE
                     AACVLGINEPPVAIKVLERSIVDHAFGQGWIRPEPPAHRTGKKVAVVGSGPAGLAAAQ
                     QLNRAGHWVTVFEKADRLGGLLRYGIPDFKMEKYLIDRRLEQMTAEGVRFQTNAHVGA
                     NVPVTELRKEFAAILLAGGAEQPRDLRVPGRELKGIHFAMEFLPQQNKRCTGDRLEGI
                     EEILATGKRVIIIGGGDTGADCLGTSHRQKALSVHQFEIMPMPPGERHASTPWPLWPM
                     QLRQESSHEEGGIREWAVSTVRFTGDENGNVKQLHGIRVSPPPAFEPLPGTEFTMDVD
                     LVLLAMGFTGPVKNGMIEQFGVSLDARGNIATGENYMSSVTGIFAAGDMRRGQSLVVW
                     AIAEGRKAARAIDKYLMGETKLP"
     gene            complement(2647..>4517)
                     /locus_tag="VFQ79_14600"
     CDS             complement(2647..>4517)
                     /locus_tag="VFQ79_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEU0140950.1"
                     /translation="GSISKEAHETLAIAMNRIGARSNTGEGGEDEERFHRDANGDWRR
                     SAVKQVASGRFGVTTNYLVNAEELQIKIAQGAKPGEGGQLPGHKVDEVIARVRHSIPG
                     VGLISPPPHHDIYSIEDLAQLIYDLKNVNPQARISVKLVAEVGVGTIAAGVSKAHADV
                     VLISGDTGGTGASPLTSIKHAGTPWELGLAETQQVLVMNDLRSRIIVQTDGKLQTGRD
                     VAIAALLGAEEFGFSTSPLISMGCIMMRKCHLNTCPVGIATQDPKLRAKFEGAPEHVI
                     NFFFYIAEEVREIMAQLGFRTMNEMVGRVDMLEMRDAVEHWKAKGLDFSSILFNPPVP
                     ARVGRRRLIPQDHGLSQALDYKLIGHAREAIQNSTPVEFKLPIRNVHRTVGAMLSGEI
                     ARQRGSEGLPEDTIRFHFTGSAGQSFGAFLAKGVTLTLEGDANDYVGKGLSGGKLIVY
                     PPRTSAFVAEENILIGNVVLYGATSGEAYFNGMAGERFAVRNSGATAVVEGVGDHGCE
                     YMTNGLVVVLGRTGKNFAAGMSGGIAYVLDETGEFSETLCNRSGVDLHPPDESDAELL
                     QALIRKHLEATSSPRAEWILDHWESTLPAFVKVFPHEYKRVLGVPRPAAETVAVVR"
BASE COUNT          776 a         1476 c         1356 g          909 t
ORIGIN      
        1 gggcgatttc ggagcttcag cgcctgcgtc cctgtcctgc gggagataaa ggcattgctt
       61 tcttcgatcc ccgctgggaa gacggtgaac atgggttgac catgatcgca aggcttgtct
      121 gccgtaaccc aactttaaaa gccgatctgg taaacgaatc cggaatggtg gccgaccgga
      181 cccgatacgc ttacaccatc acctacgacg cggcgaagaa ctcctatata gtccacggca
      241 gcgacagatt acactgaaaa actatggcga tcttgggcat cgacatcggc ggtactcgtg
      301 tcaaggcagg cttggtttcc ggtgcggggg aggtgttgcg gcggctgtcc gtgccaacac
      361 cctcttctct cgatgccttc accatagcgt tgcgagaact catcgcgaat ttactgggcg
      421 atgccggtca acctgaagcc atcggcttcg gatgcaaagg cattatcgac ttccatacgg
      481 ctcgcatcga ggtcctgccc ggcacgctgc attttctgga aggctcgaat cttgcggacc
      541 tggccggcct gtctgtcccg gtatatgccg acaacgacgc tcgcgctgcg ctcgccggag
      601 aggtggcatg gggcgccggc gcgggttgcc gcaacgcggt gatgttcacg ctcggaaccg
      661 gcgtcggcgg cggcgttctg gctgaagggc gaattctgcg cggcgaaaag ggagtcgccg
      721 gacatctcgg ccacattacg gtggacccgg acgggccggt ctgcatctgc ggcaatcacg
      781 gttgcctgga ggccgtcttt tcggcgagcg ccatcgaatc ggaagcgatc ggtgctgcct
      841 accgcggctg cgcgtcgata ctgacacgcc tgtaccggca agacccggct tcgctcacct
      901 gccaggcggt cttccaggcg gcggccgaag gcgactccgt cgccctggcg attcgcgatc
      961 gcgccgtgaa gtacctgggc gcatccatcg ccggtctgat ccacgtattc gatccggaga
     1021 aggtgatcct tggagggtta atctccgaag cgggcgatgc cctgttcggt cccgtccggg
     1081 aacatgtgcg ctggcgcacg cgggttttgc tcggccgtga agttccggtc gtgaaagcgc
     1141 aggtatcgga tagcgttgtg ggggccgcgg ccgttgcgct gctggggcgc tctctcgctg
     1201 cgccccgcgc gtgacggcgc tctacggcag cttcgtttcg cccatcagat atttgtcgat
     1261 cgcgcgcgcc gctttacggc cttccgcgat cgcccacact accagcgatt gcccacgccg
     1321 catgtcgccg gcggcaaaga tgccggtgac ggagctcatg taattctcgc cggtcgctat
     1381 attgccgcgg gcgtcgaggg aaactccgaa ctgctcgatc atgccgttct tcaccgggcc
     1441 ggtgaacccc atggccagca gcaccaggtc cacgtccatg gtgaattcgg tgccgggcag
     1501 cggctcaaac gccggcggcg ggctcacgcg gatgccgtgc aactgcttca cgtttccgtt
     1561 ttcatctccc gtgaaccgca ccgtcgaaac cgcccactcg cggataccgc cttcttcatg
     1621 cgagctttcc tgacgcaact gcatcggcca caggggccat ggagtgcttg catgccgctc
     1681 gcccggcggc atcggcatga tctcgaactg gtgcacggag agcgccttct gccggtgcga
     1741 agtacccagg caatcggcgc ccgtgtcacc gccgccgatg atgatgacac gctttccggt
     1801 tgcgagaatc tcttcgatgc cctccagacg gtcacccgtg cagcgcttgt tctgctgggg
     1861 caggaactcc atcgcaaagt ggatgccctt taactcgcgt ccgggcaccc gcagatcgcg
     1921 cggctgttcg gcgcctcccg ccagcagaat cgccgcgaac tctttccgca attccgtgac
     1981 aggaacgttc gcccccacat gcgcgttggt ctggaaccgc acaccttcgg ccgtcatctg
     2041 ttccaggcgg cggtcgatca ggtacttctc cattttgaag tccgggatgc cgtagcgcag
     2101 caggccgccg agccggtctg ctttctcgaa cacggtgacc caatgccccg cacggttcaa
     2161 ctgttgcgcc gccgccagac cggcaggccc ggagccgacg acagccactt tcttccccgt
     2221 gcgatgcgcg ggcggctcgg gacgaatcca gccctgcccg aaggcgtgat cgacgatact
     2281 ccgctccagc accttgatcg ctaccggcgg ctcgttgatg cccagcacgc aggccgcttc
     2341 acatggtgcc gggcataccc gcccggtgaa ctccgggaaa ttattggtcg agtgcagcgt
     2401 ccggatggcg tctttccaac ggtcgcgata ggcgagatcg ttgaagtcgg gaatgaggtt
     2461 attcagcggg cagccggtat ggcagaacgg cactccgcaa tccatgcagc gcgccccctg
     2521 agtttgaacg cggtcgagcg ggaagtccag gtagatttca aaccagtcgt tcacccgctc
     2581 ggtaaccgga cggcgctgcg gcaactcccg cgtgtattcg agaaagcccg tgaccttacc
     2641 catgcctcac ctcaccaccg cgaccgtttc ggccgcgggc ctgggaaccc caagcacgcg
     2701 tttatactcg tgcgggaata ccttcacaaa cgccggcagc gtcgattccc agtggtcaag
     2761 aatccactcg gcgcgcgggc tggacgtcgc ttcgaggtgt ttgcgaatta gcgcctggag
     2821 cagctcggca tcgctttcat ccggcggatg gagatccaca ccgctgcggt tgcacaacgt
     2881 ctcgctaaac tcgccggtct catccagcac gtaagcgatc cctccgctca tccccgccgc
     2941 aaagttcttc cccgtgcgcc ccagcaccac gacaagaccg tttgtcatgt actcgcatcc
     3001 gtggtcgccg actccttcca ccaccgctgt cgcgccggaa ttgcggaccg cgaaacgctc
     3061 gcccgccatg ccgttgaagt acgcttcgcc gctcgtcgcg ccatatagaa ccacgttgcc
     3121 gataaggatg ttctcttcgg caacgaaggc ggaggtgcgc ggaggataca caatcagttt
     3181 cccgccggag agtcctttgc ccacgtagtc gttcgcatcg ccttcgagag taagcgtcac
     3241 gccttttgcc agaaacgcac cgaagctctg gcccgcggaa ccggtgaagt ggaagcggat
     3301 cgtgtcctcc ggcagcccct cggatccgcg ctgccgggcg atttcaccgc tcaacatcgc
     3361 cccgaccgtg cggtgcacat ttcgaatagg aagcttgaac tccaccggcg tgctgttttg
     3421 gatggcttcc cgcgcatggc cgatcagttt gtaatccagc gcctggctga gtccgtggtc
     3481 ctgtggaatc aggcgccggc gtcccaccct cgccggcacc ggcggattaa acaggataga
     3541 actgaagtcg agccccttgg ccttccagtg ctccaccgca tcgcgcatct cgagcatgtc
     3601 gacacggccc accatttcgt tcatggtgcg gaagccgagc tgcgccatga tttcgcgcac
     3661 ttcttcggcg atgtagaaga agaaattgat gacgtgttcc ggagcgccct cgaatttcgc
     3721 ccgcagcttg ggatcctgtg tcgcgatgcc caccgggcag gtgttcaggt ggcatttgcg
     3781 catcatgatg cagcccatgg agatgagggg cgaggtcgaa aacccgaact cctcggctcc
     3841 cagcagagcg gcaatcgcga catcgcgccc ggtttgcagc ttgccgtccg tttgcacgat
     3901 gatgcggctg cgaaggtcgt tcatcaccag cacctgctgc gtttcggcca gcccgagttc
     3961 ccacggcgtt ccggcgtgct tgatggaagt gagcgggctt gcgccggtac cgcccgtgtc
     4021 gccgctgatc agcaccacgt cggcatgtgc tttcgaaacg cccgcggcga tggtgcccac
     4081 acccacttcg gccaccagct tcaccgagat ccgcgcttgc ggattcacgt ttttcaggtc
     4141 atagatcagt tgcgccaggt cttcgatgga gtagatgtcg tgatgcggcg gcggtgagat
     4201 caagcccaca cccgggatgg agtgccggac gcgcgcaatc acttcgtcca ccttgtgtcc
     4261 cggcagttgc ccgccttcgc ccggcttggc tccctgcgca atcttgattt gtagctcttc
     4321 ggcattcacc aggtaattcg tagtaacgcc gaaccgcccg gatgccacct gcttcacggc
     4381 gctgcggcgc cagtcgccat tcgcatcgcg gtggaaccgt tcttcgtcct cgccgccctc
     4441 gccggtattc gatctcgcgc cgatgcggtt catggcgatt gccagcgtct cgtgcgcctc
     4501 tttgctgatg gagccga
//
LOCUS       DASTMK010000335         5800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_143512, whole genome shotgun sequence.
ACCESSION   DASTMK010000335 DASTMK010000000
VERSION     DASTMK010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143512"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFQ79_14605"
     CDS             <1..259
                     /locus_tag="VFQ79_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140951.1"
                     /translation="LRSEASRKACGELGWSAWVLKRCLSARDIAERTPAWRAYACESF
                     CDSAACIYSGISSHPEFTLASRFRNIRLHWFSMQFSDVISV"
     gene            325..870
                     /locus_tag="VFQ79_14610"
     CDS             325..870
                     /locus_tag="VFQ79_14610"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEU0140952.1"
                     /translation="MILRAISKHWWVLTLRGVAAILFGLCALFWPALTLAVLVLLWGA
                     YSVIDGIMAVVAGISGRWWSLVFFGLIAIAAGLFAIVRPGITGLVLILVMAAWTIVRG
                     IFEIAAAVRLRKELANEWLLIVSGILSIALGILVVMFPGAGILSIIWLIAAYALIIGA
                     LLVILSFRLRRLHRMEPRRAS"
     gene            998..1798
                     /locus_tag="VFQ79_14615"
     CDS             998..1798
                     /locus_tag="VFQ79_14615"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEU0140953.1"
                     /translation="MEKMLVVVFDDEVKAYQGIQALGDLHTEGSISVFACAVISKDSE
                     GKIHVRQEVDRGPFRLVAGMAVGSLIGLLGGPVGLVIGGVTGTLLGAAGDLAKTGVGA
                     DFLEEVSQQLQPGKSAIVAEIEETWVIPVDTRMEAIGGAVIRRPRAEIIDEERARELA
                     ARRKEIDNLKIEYEQSTSTAKANAKAKLEVLESRFEALQARTKDTIESLQREGETKVE
                     AMQEQARTATEQAKANLQQRAAELRTEYERRSGKLKEALELTKDALKP"
     gene            complement(1959..2228)
                     /locus_tag="VFQ79_14620"
     CDS             complement(1959..2228)
                     /locus_tag="VFQ79_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140954.1"
                     /translation="MKRARRRIDVNLEELDRVLDGAREAPLNEADYVRTRPTHGVDGF
                     FNSAMPWRITGLSTAAANRRERSSNNLLTAENRVLIPVRSITSSG"
     gene            complement(2225..2470)
                     /locus_tag="VFQ79_14625"
     CDS             complement(2225..2470)
                     /locus_tag="VFQ79_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140955.1"
                     /translation="MAAQIGADWQRMYGHPIYFLEKFVDPERFRGTCYRAANWVVMGK
                     TTGRRKQSNSYVPNRSIKKILGYPLTRRFRELLEQSE"
     gene            2960..3550
                     /locus_tag="VFQ79_14630"
     CDS             2960..3550
                     /locus_tag="VFQ79_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU0140956.1"
                     /translation="MLSRNAAVLTLCAALWVSSCSRPAQEVNAASMTPEKDRKPAPDF
                     TLRDAGGRQVKLSDYRGKVVLLNFWATWCAPCRIEIPWFIDFEQRFKDQGFAVLGVAM
                     DDEGWEIVKPYLTEKRVNYRVLMGDEDVSEAFGGVASLPTTFMIDREGKIASVHIGLI
                     SKSDYEDEIVQLVRNAANSGAVSVSGGNRTLVARAD"
     gene            3579..3947
                     /locus_tag="VFQ79_14635"
     CDS             3579..3947
                     /locus_tag="VFQ79_14635"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEU0140957.1"
                     /translation="MVLERNAVNTATVTVQLRDGFHVNSNKPMDDYLIPLRLTWEPAG
                     VEVVEIVYPEPRLEKYDFSAKPLSVFSGSFDLITKLKVPANAQSGPGMVAGKLRYQAC
                     TEKMCFPPKTLDVKMQIEIK"
     gene            complement(3963..5618)
                     /locus_tag="VFQ79_14640"
     CDS             complement(3963..5618)
                     /locus_tag="VFQ79_14640"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU0140958.1"
                     /translation="MRLALFLGLAALLPGADFDLLIRGGRVVDGTGNSAYQADVGIKD
                     RRIAAVGWLKDKSASRVIDASGLVVAPGFIDIHNHSDYTLVEDGDAESMIRQGVTTMI
                     LGEGGSAAPLGGKQVSERDRADWKDFAGYFARLLRQGISTNVGTYVGSSQIWTYVRGP
                     QAGPPTAKELSEMRAQVDEAMRQGALGVASSLSGPPGSWIDTDTLVAMCKEAARYGGI
                     YSTHIRTEGNGVFEAVSEAIDIGRRSGVPVDIIHLKIAEHKLWGQMPELVTTIQAARS
                     RGQDVQANVYPYRAGQNNLSSIIPPWAHEGGPKAMIARLKDPKLRPRIKREILNGIPG
                     SNWYNHYTATGGWDGILLVSLSNEAYKRFEGNRMKEVIETIGRPPLDVLFELLEQNRG
                     SVPAVYFHHSEEDMRYALRQPFVSIGSDGTAVKTQGPLARGNPHPRYYGTFPRVLGRY
                     VREEQVLTLEEAIRKMTSANANKIGQFDRGLLRPGMWADVTVFDAGTVVDHSTFEKPH
                     QYSTGIEYVVVNGSVVLEKGRHTGQRPGMVLVGAGKERASQNQ"
     gene            5621..>5800
                     /locus_tag="VFQ79_14645"
     CDS             5621..>5800
                     /locus_tag="VFQ79_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140959.1"
                     /translation="MHHKLPWGSLVPQLSRDGNGVPMGRQPAEQNESGLEWQRCGAAE
                     AGTAVDQSKPYGVWSG"
BASE COUNT         1145 a         1668 c         1746 g         1241 t
ORIGIN      
        1 gcttcgaagc gaagcaagca gaaaggcctg cggcgagctt ggctggtccg cctgggtgct
       61 gaaacgctgt ctgtcggcgc gggacatcgc cgaacgaact ccggcgtggc gcgcctacgc
      121 ttgtgagagc ttttgcgatt cggccgcgtg catctactcc ggaatttcgt cgcacccgga
      181 atttacgctc gcctcacgtt tccggaacat ccggttgcat tggttctcga tgcaattcag
      241 cgacgtaatc tctgtataat gtccgattga cagccaagac gaccggtagt atcctaagcg
      301 gcgatatcca tcgaggtata cgttatgatc cttcgagcta tttctaaaca ctggtgggta
      361 ctgacactgc gcggcgttgc ggctattctg ttcggcctgt gtgcattatt ctggccggcg
      421 ctgacgctcg ctgtcctggt gttgctctgg ggtgcgtatt cggtgattga cggcattatg
      481 gccgtggtcg caggtatatc gggacgatgg tggtcgctgg ttttctttgg actgatagcc
      541 atcgccgccg ggttatttgc gatcgtccgc cccggcatca caggcctggt cctaatcctg
      601 gtaatggcgg cgtggaccat tgtgcggggc atcttcgaaa tcgccgccgc cgttcgcctg
      661 cggaaagaac ttgccaacga gtggctgctg atcgtaagcg ggattctctc aatcgcgctt
      721 ggaatattgg tagtaatgtt tccgggtgcg ggtattcttt ctatcatctg gctcatcgcg
      781 gcgtacgctc tgataattgg cgcgctctta gtcattctgt cgttccgcct ccggcggttg
      841 caccgaatgg agccacgccg ggcatcctga aggatgcgcc gttacgagac gacttcaacc
      901 acagccgatc ttgctgcgca gcgctgtttg atgtacccag actacacgaa agactctcgg
      961 aaaaactgat ataacgataa ctgcacagga ggtgcatatg gagaaaatgc tagtggttgt
     1021 gtttgatgat gaggttaagg catatcaagg catccaggcg cttggagatc tccacaccga
     1081 aggcagtatc tcggttttcg cgtgcgccgt aatctcaaag gactcggagg gcaagattca
     1141 tgtgcggcag gaagtcgaca ggggaccgtt ccgtctggtt gcgggaatgg ccgtaggatc
     1201 tctgatcggt ctgctcggtg gaccagtggg tcttgtcata ggtggagtga cgggcaccct
     1261 acttggcgcg gcaggagatc tggcgaagac cggtgtgggc gccgatttcc tcgaagaggt
     1321 ctcacagcag ttgcagccgg gaaagtctgc gatcgtggcc gagatcgaag agacgtgggt
     1381 cattcccgtc gatacgcgca tggaggcaat cggtggcgcg gtcattcgcc gtccgcgagc
     1441 cgagatcata gacgaagagc gcgcgagaga gctcgcggca cgaagaaaag aaatcgataa
     1501 tctgaaaatc gagtacgaac agagtacgtc tacagccaag gcgaacgcca aggccaagct
     1561 ggaggtcctc gaaagccgct ttgaagccct tcaggcccga acgaaggaca cgatcgagtc
     1621 gcttcaacgc gaaggagaga caaaggtcga agcgatgcag gagcaggcgc gaacggccac
     1681 ggaacaggcc aaggcgaacc tccagcagcg ggccgcggag ttgcgcaccg agtacgagag
     1741 gcggtccggg aagctcaagg aagctctcga actcacgaaa gacgcactga agccctgagg
     1801 tggggttgcg gacgtatcac ttattaaggc tccgtcgtct cgggcgacag catttgggac
     1861 ttgcttgccg cgaagcgtgc cagtgcgctt tcggttggct tgctgtcggg aggcttcgga
     1921 tcgttaagaa ataatgtttc caatctagaa cgttttggct atcctgacga ggtgatagac
     1981 ctcaccggga tcaagacacg gttctctgct gtgagcaggt tgttggacga gcgttcgcgc
     2041 cggttggcgg cagcggtcga gagtccggtg attcgccagg gcatcgcgga gttgaagaac
     2101 ccgtcaacgc cgtgggtggg gcgagtgcgg acgtaatcgg cttcgttcaa cggcgcttcc
     2161 cgggcgccgt ccagcacccg gtccagttcc tccagattca cgtcgatgcg tcgtctggcc
     2221 cgcttcattc cgattgctcc agcagttcgc gaaaacgcct ggtaagtgga tatcccagaa
     2281 tctttttgat tgaccggttc ggcacgtagc tgttggattg cttgcggcgc cccgtcgttt
     2341 tgcccatcac cacccagttc gccgcccggt aacacgttcc gcggaatcgc tccgggtcca
     2401 cgaacttctc cagaaagtag atcggatgcc cgtacatccg ctgccaatcc gcgccgatct
     2461 gtgccgccat gcggccgcgc gcgttgcgcc agtggattgt ggcgcttgta agcggccggc
     2521 ggcaaggcga tctcgccagc ccgctccagc atcagcagca aaccgcgaca taccatgtcg
     2581 cgcagggcac cattgctttc cgttcgcctc tatcattttt ctggcgtttt ccgaagccgg
     2641 caacacggcg tgcaggtgat gctcaaggca ggatctgctt cgaagcgaag caagcagaaa
     2701 ggcctgcggc gagcttggct ggtccgcctg ggtgctgaaa cgcggtcttt cggcgcggga
     2761 cgtcgccgaa cgaactccgg cgtggcgcgc ctacgcttgt gagagctttt gcgattcggc
     2821 cgcgtgcttc tactccggaa tatcgtcgca cccggaattc accctggcct cccgtttccg
     2881 taacatccgg ttgcattggt tctcgatgca attcagcgac gtaatctcta tataattgaa
     2941 aaaggagtcc gccacaccta tgctttcccg caacgccgct gttctgacct tgtgtgccgc
     3001 gctttgggta agctcgtgct cgcgaccggc gcaagaggtc aacgccgctt ccatgacgcc
     3061 tgaaaaggac cggaaaccgg cgccggactt tacgctgcgc gatgccggcg gcaggcaggt
     3121 gaagctctcg gactatcgcg gcaaagtcgt tttgttgaat ttctgggcga cgtggtgtgc
     3181 tccgtgcaga atcgaaatcc cctggtttat cgatttcgag cagcgtttca aggaccaggg
     3241 gttcgcggtg ctcggcgtcg ccatggacga cgaagggtgg gagattgtga agccctacct
     3301 tacagagaaa cgggtgaact accgggtgct catgggggac gaggacgtat cggaggcctt
     3361 cggcggcgtg gcctcgctgc cgacaacgtt tatgatcgac cgcgagggca agatcgcttc
     3421 cgttcacatt gggcttataa gcaagagcga ttacgaagat gaaattgttc aactggtcag
     3481 gaatgccgcg aattccggcg ctgtgtctgt tagtggcggc aaccgcactc tcgttgcccg
     3541 cgcagactaa cgttctcagc atcaagacag ccccgcggat ggttctggaa cggaatgcgg
     3601 tgaacaccgc aacggtaacg gtccagcttc gcgatggctt ccacgtcaac agcaacaagc
     3661 cgatggacga ctaccttatt ccgctgcgtt taacctggga acccgcggga gttgaggtgg
     3721 tggagatcgt ataccccgag ccgcggctgg agaagtacga cttttccgcc aagccgctgt
     3781 cggtattttc cggcagcttc gatctgatta cgaaactcaa agttccggca aacgcacaat
     3841 ccggccccgg catggtagca ggtaagttgc gctatcaggc ctgcaccgaa aaaatgtgct
     3901 ttccgccgaa gactctcgac gtcaagatgc agatcgagat caagtagacg ccccggagcg
     3961 ggttattgat tttgactcgc ccgttctttg ccggcgccca caagcaccat ccccggccgc
     4021 tgtcccgtgt ggcggccctt ctccagcaca acggagccat tcactacgac gtattcgatg
     4081 ccggtggaat actggtgcgg tttttcaaag gtagagtgat ccaccaccgt tcccgcgtcg
     4141 aacacggtga catccgccca catgcccggg cgtagcagac ctctatcgaa ctggccgatc
     4201 ttgttcgcgt tcgccgacgt cattttgcga atggcttctt ccagcgtcaa aacctgctcc
     4261 tcgcgaacat aacgccccag cacgcgcggg aatgttccgt agtagcgcgg gtgcggattg
     4321 ccacgggcca gggggccctg ggtttttacc gccgttccat cggagcctat tgaaacaaac
     4381 ggctggcgca gcgcatatcg catgtcctct tcggaatggt gaaagtagac ggccggcacg
     4441 gacccgcgat tctgctccag caattcaaaa agaacatcga gaggcgggcg gccgatcgtc
     4501 tcgatcactt ccttcatgcg attgccctcg aagcgcttgt aagcctcgtt cgatagcgat
     4561 accagcagaa tgccgtccca gccgccggtg gcggtgtagt ggttgtacca gttgctgccc
     4621 ggtattccgt tcaggatttc gcgcttgatc ctcggccgca gcttgggatc tttcaaccgt
     4681 gcgatcattg ccttcggccc accctcatgg gcccagggag gaataatact cgagagatta
     4741 ttctgtccgg cacggtaagg gtatacgttc gcctgcacat cctgcccgcg cgatctggca
     4801 gcctgaatag tggttaccag ctccggcatc tgtccccaca gcttgtgctc ggcgatcttc
     4861 aggtgaatga tgtcgacggg tacccccgaa cgccttccga tgtcgatggc ttcgctgacg
     4921 gcttcgaaaa ccccattccc ctcggttcga atatgggtgg agtagatccc tccatatcgc
     4981 gccgcttcct tgcacatcgc gaccagcgtg tccgtgtcga tccacgaccc gggaggaccg
     5041 ctcagcgaac tcgccacgcc taacgccccc tggcgcatcg cttcgtccac ctgcgcgcgc
     5101 atttcagaga gttctttcgc tgtagggggt cccgcctgcg ggccgcgtac gtatgtccag
     5161 atctggctcg acccgacgta ggtaccgacg ttcgtggaaa ttccctgccg caggagccgg
     5221 gcgaagtatc ccgcgaaatc cttccagtcc gcgcgatccc gctccgagac ctgcttgcct
     5281 ccgagaggag cggcagagcc gccttcgccc agaatcatgg tggtcacacc ctggcggatc
     5341 atgctctcgg cgtcgccgtc ttccaccagt gtgtagtcgg aatgattgtg gatgtcgatg
     5401 aatcccggcg cgacaaccag gcccgatgcg tcgataacgc gcgaggcgga cttgtccttc
     5461 agccatccca cagccgcgat tctgcggtct ttgattccta catccgcctg gtaagcactg
     5521 tttccggtgc catcgacaac gcgcccaccg cggatcagca ggtcgaaatc cgccccaggc
     5581 aaaagagccg ccagtccgag aaataaggca agtctcatag atgcatcata agcttccgtg
     5641 gggctcgctg gtgccgcaac tgagccgcga cggtaacgga gtgcccatgg gccggcagcc
     5701 cgccgaacaa aatgaaagtg ggttagagtg gcagcggtgt ggggcggccg aggccggaac
     5761 cgctgtggat cagtcgaagc cgtacggagt ttggtctgga
//
LOCUS       DASTMK010000336         8876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_528943, whole genome shotgun sequence.
ACCESSION   DASTMK010000336 DASTMK010000000
VERSION     DASTMK010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528943"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2308)
                     /locus_tag="VFQ79_14650"
     CDS             complement(<1..2308)
                     /locus_tag="VFQ79_14650"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEU0140960.1"
                     /translation="MVQLCFRLRPMLAVVLVVSALATGALFSIRAQSATDPGVDFQKD
                     VQPVFQASCYPCHSAKVASGDLRLDNKNLALQGGVSGRPILPGKSGESLLLRRLTGED
                     KGARMPLGGALPADKIALIRAWIDQGAQWPEEAPAAKTTSKHWAYVKPVAPPVPAVTD
                     AKWVRTPIDNFVLARLEREKLAPSPEASRETLIRRLSLDLTGLPPSIAEVDAFVADQS
                     PDAYTKLVERLLASPHYGERWARPWLDMARYADTNGYEADRQRTIWKYRDWVIGAFNR
                     DIPFDEFTIEQIAGDMLPDPSNDQLIATGFHRNTMFNEEGGVDKEEAHWTNLVDRVNT
                     TTTVWLGSTIGCAQCHNHKFDPFTQREYYQLLAFFDNGAFTVKTYGDTSNKFIEPQLE
                     MPTPEQEARRKKLNAGIDELEAKTKTQTPELDAEQAQWERTVRQASSGWQTLVPAKIT
                     SSGGSTLTLQQDHSILASGSNPAEDTYTIEAKLSEGRITAVRLETLPHETLPRGGPGR
                     DAYGNFFLSEVALEMEGNELCIKKAVPDDGRMNDKKFGQLWAVDASRDEKRLPRQLVL
                     LLDTPVEGGGTLRVHLRHRSEFGGQGVGHFRLSVTSSQEPDQIVKVSARLRPVLELDS
                     GARTEKQKNDLAEYFRANAPSLKATRDRLKKLRDERDDLGIVSTLILRERNSFDRPST
                     LMRSRGEFLSKGDRVWAGVPAVLHPMPQNAMPNRLGLAKWLVSPENPLTARVTMNRIW
                     EQYFGRGLVETSEDFGAQGERPTHPELLD"
     gene            complement(2394..3080)
                     /locus_tag="VFQ79_14655"
     CDS             complement(2394..3080)
                     /locus_tag="VFQ79_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140961.1"
                     /translation="MLDKSPGRSDLVISMTLAETFLLLVFVVWYGEVAAGKDTGTLPD
                     WQKVAEQRQKEIDRLKADVEREKSARALGDAALKWWQENFRIGPPGSAEELVEALRTP
                     AGKAVLMELARGYPRCEQDNVLVHASVLNGRVRMRVVGASERLRAWCQRSGIALPKTK
                     SELADWRSIRAFLDVIRRFYGSEPVGGKACRFDYQLSYQTKADYYDGRESFEKSFYAA
                     RIARAPAGGD"
     gene            complement(3093..4475)
                     /locus_tag="VFQ79_14660"
     CDS             complement(3093..4475)
                     /locus_tag="VFQ79_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140962.1"
                     /translation="MTTSRWLARVEGQKLGFYWAIAAIVGAASISASAAEKPQRLLLP
                     ILIMIAYAWAGYRNARFSASHPALHSARVAQLADSVYFLGFLWTLWALIDSFVLKSMS
                     GAQGVFRVFGYALVTTATGMFLRLLLLQFRYTAQDQAAEVEFRVEEQLEKFSAQLTRT
                     ARSLEEWSEAFARLTAALNSNVQRIGEQTADLEQQLKEVHRQSLEELYRNISAGIQNF
                     GELLRPRVDELSSASAEIEQALSAGAQGIERGMKDAGERLRASEIPAQIDAAMRRLAV
                     KVEEIETALAALAAGIRAGGQVTEAVGQLPESLRTGAARIGEELEQLAVRIREIRVPD
                     DVEDKSRVFKERVAEVEEGLRAVAAAFRNGSQTVTSTVTVEVQSGAETIRREIDGFAK
                     RIGEFGAPLQTANRDAALERSIQDLESAISLLADRLPPQARVRAEGRAKRGLRAGIGW
                     LTWPFRRRSG"
     gene            complement(4487..5818)
                     /locus_tag="VFQ79_14665"
     CDS             complement(4487..5818)
                     /locus_tag="VFQ79_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140963.1"
                     /translation="MTVKRSLYIALLVAIVSACVAPTACTRARSPEGTSVVVLIDFSK
                     SFAPLTVSDARALNEVASAIVELAAKSWSPPVRFRWARIEEASVTAKNLCGPLEFESK
                     LIKSDNDLDRAGLRQKLAECASTVLRASQDTSQQSSFTDISGAIALAAQDRENTGEKI
                     LVIFSDFLEDQRAGLQPVDFQLHGERVLLIHRPGLDLRGLTVGEYMARIDEWAGKLRL
                     RGAKEVTSIPVFGITEHRVANALAPPAKPDGTAVSIVTSLPDTAEADVLLHVARAISA
                     SSPKWIAPVTVTWSAIEQSALVARWMPPVELQSRLIKRMGLLSPADEFAIQLEQCARG
                     IARFPPTVEAADLPGALALHRQVAALDSRRLLLVLSDFRGALVDGAAPVDLSGTEIAM
                     IAAPSRSDTAHQQNYFQRLKLWEQAFRECGARVVCRIQMSDLTAGAISRCL"
     gene            complement(5824..7674)
                     /locus_tag="VFQ79_14670"
     CDS             complement(5824..7674)
                     /locus_tag="VFQ79_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140964.1"
                     /translation="MDGFGQFLFGYVPWLRPVVAWILAALIVFLLLTLPSYFILLPFA
                     RRIRTEIATYLSKLAARHEKASAARDEKLDRITGSLCEQRDPRQLNARNQFLYLRCLQ
                     RMQGALAPLRKSLDKTTAKLARAAKTVERVPERLRDAQPAQVASVELPAAGELQASVA
                     RVRAARTRLVITSVLLLSLVLVNTGMLSQIIRDLGFIPPSLVFLDIPLYVLFALFLTL
                     LEAGLGYIHAATGGDPDDTDRVHLWPYVAIVLAVAIASVEGFFYSQVAPSRDSMVEFP
                     FIGYEIKQAHLFFVWGFALVMVLFGLGSVWSNALDTVRRGNALTELRSRLKKTRKEAE
                     RSAKAAAGAVEATTNVTELSHRIGALIGAEANRTTTLRGEVARLDAQLETMHREPPEW
                     ADMTERPLTRADVQQLAAQSAMWTALLLLAGLILTFTGFESVTGLNPGIPHALCWAIA
                     LGQVLVFTGTGFLLGAGATVVQGTGNERVVLAGPRWSRALAITLAALLTVTYIAVAFL
                     VPMARYREALWICNLLLGYFLIAAGYQLAPLLGVSRLWLGRLAAAVANTLQALHAFIV
                     RILLMISAVLENLFFLLASPVATVIELLRGDQRTTEPPPEPLVHRQAAPR"
     gene            complement(7820..8629)
                     /locus_tag="VFQ79_14675"
     CDS             complement(7820..8629)
                     /locus_tag="VFQ79_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140965.1"
                     /translation="MQLQNRLAIIIGGVLLMTVACSREAPPPQASQDQPPPSEQQAPA
                     ATPAPVAPPRETAKERPAARPPARQPESRVAESKPQTPVTEPPPARPEPVAPQPVTAT
                     LEAGTQLRIRTIEAISTKTAKKGDTFSGTLVDALRDGDRLIAPRGANVAGIVTESDPG
                     GRVKGRASLSVQLTRLGTDYGGPVDISTGAVSVEAQGSKAKDARNIGIGAGIGAAIGA
                     IAGGGKGAAIGAGAGAAAGTGATLATHGEAATIPSETVLTFELRRPVTVRK"
BASE COUNT         1643 a         2789 c         2652 g         1792 t
ORIGIN      
        1 agtcgagcag ctcggggtgg gtcgggcgtt ctccttgagc gccgaaatct tcactcgttt
       61 cgaccaaccc gcgtccgaag tactgctccc agattctgtt catggtcaca cgcgcggtga
      121 gaggattctc gggacttacc agccattttg ccaggccgag gcggttcggc atcgcattct
      181 gcggcattgg atgcagcacc gccggaacac cggcccatac ccggtcgccc ttgctcagaa
      241 actcgccgcg ggagcgcatt aacgttgaag gccggtcgaa cgaattccgc tcgcgtaaga
      301 taagcgtgct tacgataccc agatcgtcgc gctcgtcgcg cagtttcttc aggcggtcgc
      361 gcgtggcttt aagggacgga gcattggcgc ggaagtattc cgccagatca tttttctgct
      421 tttccgtgcg agcgccggaa tcgagttcca acacaggacg cagcctcgcc gagactttca
      481 caatttgatc cggctcttgg gaggaggtga ctgacagccg aaaatggcct acgccctgcc
      541 caccgaattc cgaccgatgc cggagatgga cacgcagcgt gccgccgcct tcgaccggcg
      601 tatccagcaa cagcaccagt tgacgcggca ggcgcttttc gtcgcgactt gcgtcgacag
      661 cccaaagttg gccgaacttc ttgtcgttca ttctgccatc gtcgggcacg gcctttttga
      721 tgcacagttc attgccttcc atctccagtg cgacttcact gaggaagaaa ttgccgtagg
      781 cgtcccgtcc gggcccgccg cgaggcaatg tttcatgtgg caaggtctcc agacgcaccg
      841 ccgtaatgcg gccttccgac agtttcgctt cgatcgtgta agtgtcttca gccgggttgc
      901 taccgcttgc caggatcgaa tggtcctgct ggagcgtgag ggtagaacca ccactcgacg
      961 ttatctttgc cggtaccagc gtctgccatc ccgaagaggc ctggcgaacc gtgcgctccc
     1021 actgcgcctg ctcggcgtcc agttcgggtg tctgagtctt tgtcttcgcc tccagttcgt
     1081 cgatcccggc gttgagtttc ttccgccgcg cttcctgttc cggagtaggc atctccaact
     1141 gcggttcgat gaacttgttg gatgtgtcac cgtaggtttt gacggtgaat gcgccgttat
     1201 cgaagaacgc gaggagttga tagtattcgc gctgcgtgaa cggatcgaac ttgtgattgt
     1261 gacactgggc gcagccgatg gtgctcccga gccagaccgt cgtggtggtg tttacccggt
     1321 ccaccaggtt cgtccagtgc gcctcctctt tgtccacacc gccctcttcg ttgaacatgg
     1381 tgttgcggtg aaatcccgtg gcgatcagtt gatcgttgga gggatccggc aacatgtcgc
     1441 cggcaatttg ttcgatggtg aactcgtcga acggaatgtc acgattgaag gccccaatca
     1501 cccagtcccg gtatttccag atggtccgct gacggtcggc ctcgtatccg ttggtatccg
     1561 cataacgcgc catgtcgagc cagggccgcg cccatcgctc gccgtagtgc ggcgacgcca
     1621 gcagcctctc gacaagcttt gtgtaagcgt caggactttg atcggcgacg aatgcgtcga
     1681 cctcggcgat gctcggaggc aatccggtca ggtccaggct caacctgcgg atgagagttt
     1741 ccctgcttgc ttcgggagac ggagcgagct tctcgcgctc caggcgtgcg agcacgaaat
     1801 tgtcgatcgg ggtgcgcacc catttcgcgt cagtcacggc gggaacaggc ggcgctacag
     1861 gtttcacata tgcccaatgc tttgaggtgg tctttgcggc tggagcctct tccggccact
     1921 gtgctccctg atcgatccac gcgcggataa gggcgatctt gtccgccggg agcgctccgc
     1981 ccagcggcat gcgggcacct ttgtcctcgc ccgtcagccg ccgcaacagg aggctttcac
     2041 cgctcttacc cggcagaatc ggcctgccgg aaactccccc ttgaagcgcg agattcttat
     2101 tgtccagccg caagtcgccg ctggctactt tggcggagtg gcaggggtaa caggaggctt
     2161 ggaatacggg ctgaacgtct ttctggaaat cgactccggg atctgtggca gactgcgccc
     2221 ggatggagaa tagcgcgccg gtggcgaggg cggaaacaac aagcacgacg gcaagcatcg
     2281 gccgaaggcg aaaacaaagt tgaaccatga ccagtcccag cccggaaatc cgtcgaatta
     2341 gcgggtgtct atattctata ttctatcgtg ccaggggcgg cagtggagat gcgttaatcg
     2401 cctccggccg gtgcccgcgc gattcttgcc gcatagaagc tcttctcaaa gctttcacgg
     2461 ccatcgtaat agtcggcttt cgtttgatag ctcaactgat agtcgaagcg gcaggccttg
     2521 ccgcccaccg gctcggaacc atagaaacgg cggatcacat ccaggaatgc gcgtatggag
     2581 cgccagtccg ccagctcgct tttcgtttta gggagtgcga tacctgagcg ttggcaccac
     2641 gctctcaacc gctcgctggc gccgacgacg cgcatacgga ctctgccgtt gagcacggat
     2701 gcatgtacga ggacattgtc ctgttcgcaa cgcgggtagc cgcgagcgag ttccatcaga
     2761 acagccttac ccgcaggagt cctcagagct tccaccaact cttcggccga acccggtggg
     2821 ccgatgcgga agttttcctg ccaccacttg agggctgcgt ctccgagggc ccgcgccgac
     2881 ttctcccgct cgacgtccgc cttcagccgg tcgatttcct tctggcgctg ctcggcgacc
     2941 ttctgccaat cgggcagagt accggtatct ttcccggcag cgacctcgcc gtaccagacg
     3001 acgaacacca ggagcaaaaa tgtctccgcc agcgtcatgg aaatgaccag gtccgacctg
     3061 ccgggggatt tgtccagcat gattgccttc ctctatccgg agcgccgccg gaacggccag
     3121 gttagccatc cgatgcctgc tcgaaggccg cgcttggctc ggccttccgc gcgcacgcga
     3181 gcctgcggcg gcaatcgatc agctaaaagc gaaatggcgg actccaggtc ctgaatgctc
     3241 cgctccagag cggcatctct gtttgccgtc tgtaggggag caccgaattc gccgatgcgc
     3301 ttcgcaaagc cgtcgatctc gcgccggatc gtttcggctc cggactgcac ttcaaccgtc
     3361 accgtgctgg tgacggtttg cgacccgtta cgaaacgccg ctgctaccgc tcttaagccc
     3421 tcttccactt cggcgactct ctccttgaac acccggctct tgtcttccac gtcatccggc
     3481 acacggatct cgcgtattcg aactgctagc tgttccaact cctcgccgat gcgcgccgcg
     3541 cctgttcgaa gactctccgg caattgcccc accgcttcag tcacctggcc accggcgcgg
     3601 attccggcgg caagcgctgc cagagcggtt tcaatctctt cgaccttcac agccagacgg
     3661 cgcattgcgg catcgatttg cgcgggaatc tcgctcgcac gaagacgctc gccggcgtct
     3721 ttcatgcctc tttcgatgcc ctgtgcaccc gcggacaaag cctgctcgat ttccgcgctc
     3781 gcgctgctca gttcgtccac tctcggccgc agcagttccc cgaaattctg tatgcccgca
     3841 ctgatgttcc gatataattc ttccaggctt tggcggtgaa cctccttcag ttgctgttcg
     3901 agatccgccg tctgctcgcc gatgcgttga acgttcgagt tcagggcggc ggtaagtctg
     3961 gcgaatgctt cgctccactc ctcgagcgag cgggccgtgc gggtgagttg agctgaaaac
     4021 ttctcgagtt gttcctccac ccgaaactcg acctcagccg cctggtcctg tgccgtgtag
     4081 cgaaactgaa gcagcagcag gcgcaggaac atgccggtgg cagtggttac cagtgcataa
     4141 ccgaacaccc ggaaaacacc ctgggctcca ctcatgctct tcagaacgaa ggaatcgata
     4201 agggcccaga gcgtccacag gaatcccagg aaatagacgg aatcggcgag ctgggctaca
     4261 cgcgcggagt ggagcgcggg atggctggcg gagaaacgcg cattgcgata tcccgcccac
     4321 gcgtaggcaa tcatgatcag aatgggcaac aggagccgct gcggtttttc agcggccgag
     4381 gcgctgatag atgctgcccc gacaatggcg gcaatggccc agtagaagcc gagcttctgc
     4441 ccttcgacac gggccagcca gcggctggtc gtcattcccg ctcccatcac aggcatcttg
     4501 aaatggcccc cgccgtaaga tcggacatct gaatgcggca gaccacgcgc gcgccgcatt
     4561 cacggaatgc ctgctcccac agtttcagcc tttggaaata gttctgctga tgcgcggtgt
     4621 cgcttctgct gggtgctgcg atcattgcga tctccgtgcc ggagagatcg accggcgccg
     4681 ctccatcgac gagcgcgccc cgaaaatccg acaacaccaa taacaatctg cgggaatcca
     4741 gcgcagccac ctgcctgtgg agtgcaagcg cgcccggaag gtccgccgct tccaccgttg
     4801 gagggaaacg cgcgatacct cgtgcgcact gctccagttg aatcgcgaac tcatcggccg
     4861 ggctcaacag gcccatccgc ttgatgagtc tcgattgcag ctcaacaggc ggcatccacc
     4921 gggccaccag cgccgattgt tcaatggcgg accacgttac ggtcacaggc gctatccatt
     4981 tcggcgagga agccgatatg gcccgggcta cgtgcaacag aacgtccgct tcggcggtat
     5041 cgggcaaact cgtaacgatg gagacagccg tgccgtccgg ctttgcggga ggtgccagcg
     5101 catttgccac gcgatgctca gtgatgccga acaccggtat gctggtcact tccttcgctc
     5161 cccgtaaccg tagttttccg gcccattcgt cgatgcgagc catgtattcg cctacagtca
     5221 acccgcgcag gtccagaccc ggacgatgaa tgagcagcac ccgctcgcca tgaagctgga
     5281 aatcgaccgg ctgcaacccg gctcgctggt cttccaggaa gtccgaaaag atgaccagta
     5341 ttttttcgcc ggtgttctcc cgatcctggg ccgccagggc gatagcgccg ctgatgtcgg
     5401 tgaatgaaga ctgttgagag gtatcctggc tggcacgtaa gacggtactg gcacattcgg
     5461 ccagtttctg gcgcaggccc gcccggtcca gatcgttgtc gcttttaatg agcttgcttt
     5521 cgaattcgag cggtccgcag aggttctttg ccgttacgct ggcctcctca atgcgcgccc
     5581 agcggaagcg caccggcggg ctccacgatt tcgcggcgag ttccacgatg gccgatgcca
     5641 cctcgttcaa agcgcgggcg tccgatacgg tcagaggcgc gaagctttta ctgaagtcta
     5701 tgaggacaac gacagaggtg ccttcggggc ttcgggctcg cgtgcaggcg gtcggcgcca
     5761 cgcacgcact cactatggcg actagcaggg cgatatacaa gctgcgcttc actgtcatca
     5821 agctcatctg ggggcggcct ggcgatgcac aagcggctcg ggcggcggtt cggtggtccg
     5881 ctggtctcct cgtaatagct caatcaccgt cgcgaccggc gaagcaagca ggaaaaagag
     5941 gttctcgagc accgcagaga tcatcagaag aatccggacg atgaaggcgt gcagagcctg
     6001 tagagtgttt gcgacggcag ccgccaacct gccaagccac agccgggaca cacccaataa
     6061 cggcgcgagc tgataaccgg cagcaatcag aaagtagccc agtagcagat tgcagatcca
     6121 aagtgcttcg cggtagcgag ccatcggaac aagaaacgca acggcgatat atgtcaccgt
     6181 cagcagggcg gctaacgtaa tcgcaagagc cctgctccag cgcgggccgg ccagcaccac
     6241 ccgttcgttt ccggtgccct gtaccaccgt cgctcccgcg ccaagcagga atccggtgcc
     6301 ggtaaacaca agcacctggc cgagagcaat cgcccagcat agggcgtggg ggatgcccgg
     6361 gttcagcccg gtgacgcttt cgaatcccgt gaatgtcagt atcaggcccg cgagcagaag
     6421 caatgccgtc cacatggccg attgcgccgc cagttgctgc acgtcggctc gcgttaacgg
     6481 acgctctgtc atgtccgccc attcgggcgg ctcgcggtgc atcgtttcca actgtgcatc
     6541 gagccgtgct acttcgccgc gcagggttgt ggtgcggttc gcttcggcac cgatcagcgc
     6601 cccgatgcga tgtgagagct ccgtgacgtt cgttgtggct tcgaccgctc cggccgcggc
     6661 cttcgcgctt cgttccgcct cttttctcgt tttcttcagt ctgctccgca gttccgtaag
     6721 cgcgttcccc cggcggaccg tgtcgagtgc gttcgaccag acagacccca gcccgaatag
     6781 aaccatcacc agtgcgaatc cccaaacgaa gaagaggtga gcctgcttga tctcgtatcc
     6841 gatgaacggg aattcgacca tcgagtcacg gcttggcgcc acctgcgaat agaagaaccc
     6901 ctcgacggac gcgatggcaa cagccagcac aatggctacg taaggccaca ggtgcacgcg
     6961 atcggtgtcg tcgggatctc ctcccgttgc ggcgtgaatg tagccgaggc ccgcttccag
     7021 caaggtcaga aacaaggcga aaagcacata tagaggaatg tcgagaaaga ccagcgaggg
     7081 cggaataaag ccgaggtccc gtatgatctg gctcaacatg ccggtgttca ccagcaccag
     7141 gctgaggagc agcacgctgg taatgaccag gcgcgtacgc gccgctcgaa cccgcgcaac
     7201 agaggcctgg agttcgccgg ccgcgggtag ctcaacggat gcgacctgtg ccggctgtgc
     7261 gtctctcagg cgctcgggta cccgttccac cgttttcgcg gcacgcgcca gcttggcggt
     7321 tgtcttgtcg agcgattttc tgagcggcgc caacgctccc tgcatgcgct gcaagcagcg
     7381 gagatacagg aactggtttc tggcattcaa ttgcctgggg tcgcgctgct cgcaaaggct
     7441 gccggtaatg cgatccagtt tctcgtcgcg ggcggcggac gccttctcgt gccgtgcggc
     7501 taatttcgag agataggttg cgatctcggt ccggatccgc cgcgcgaacg gcagtagaat
     7561 gaaatacgac ggtagggtca ggagaagaaa gacgatcagc gcggccagga tccaggcgac
     7621 caccggacgt aaccagggca cataaccaaa gagaaactgt ccgaagccat ccatacggag
     7681 ctcctcccaa atgctgcgga cgcagaaccg atgatatgcc tggagaagct gcgatgcaag
     7741 gagagctggc gcacttcacg cggcgggagg tccgcggttg gcagatctcc ccgccggcgc
     7801 agcggagttg ctggatttgc tacttgcgga cggtcaccgg cctgcgcagt tcgaaggtca
     7861 gcaccgtttc cgaggggatc gtcgccgctt cgccgtgcgt agccaatgtc gctccggtgc
     7921 ccgctgccgc tccggcgccc gcaccgatgg ccgcgccctt gccgcccccc gctatcgctc
     7981 caatggctgc gccaatgccc gcgccaatcc cgatattccg tgcgtccttc gccttcgacc
     8041 cttgagcctc caccgacacg gccccggtgc tgatatccac gggcccaccg taatcggtcc
     8101 caaggcgggt cagttggacc gacaacgaag cgcggccctt cacccttccg cctgggtctg
     8161 attccgtcac aataccggcc acgtttgcgc ccctgggcgc gatgagccgg tccccatccc
     8221 gcaatgcgtc gacgagcgtg ccgctgaacg tgtctccttt tttggctgtc ttggtggata
     8281 ttgcttcaat ggtgcgaatc cgcaactggg tgccggcttc aagcgtggcg gttaccggtt
     8341 gcggtgcgac cggctccgga cgcgccggcg gcggctccgt aaccggtgtt tgcggcttgc
     8401 tctcagccac ccgcgattcc ggttgccgcg ccggagggcg tgcggcaggc cgctctttcg
     8461 ccgtttcccg cggcggggct accggagcag gcgtcgctgc cggcgcctgc tgttcggaag
     8521 gcggtggttg atcctggctg gcttgcgggg gcggagcctc acggctgcac gcaaccgtca
     8581 ttagaagcac gcctccgata atgatcgcta atcgattctg tagttgcatc tcatccccct
     8641 ttcagtagcc gtgcaaccgt ctacaattat gacatagcgg tcagccggcg ctgtccctgt
     8701 tcatggagac tgatggcgtc ctgttgttgc gcacaaccgc tctctcgaaa gttactccgg
     8761 aaaaatcacg gctgtgcccc tttggcgtcc ggcgaggtga tctggacggt gtagccggca
     8821 ttccgaagtt cctgggtgag cctttggata cggcgcttca tggcctgcgg ggtggt
//
LOCUS       DASTMK010000337         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_497026, whole genome shotgun sequence.
ACCESSION   DASTMK010000337 DASTMK010000000
VERSION     DASTMK010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497026"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFQ79_14680"
     CDS             complement(<1..293)
                     /locus_tag="VFQ79_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140966.1"
                     /translation="MADRMSDPALDTLGYKPQFRRVLSRGDLVLYGLVILTPTAAYPV
                     FGIVQQVSHGHAALSYLVALVAMLFTASSYGKMAGAFPAAGSTYTYAQRSLSE"
     gene            complement(293..2506)
                     /locus_tag="VFQ79_14685"
     CDS             complement(293..2506)
                     /locus_tag="VFQ79_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEU0140967.1"
                     /translation="MFDRLVLCLLCATALPAQPLTDLWLRGYSVIPTPRKVQLDTRDV
                     VVDSSWRIDAGAVGSQHIAVRALIRDLQDFHGLRFHPPSGGVVIRLAIQAGAVKTGRE
                     PEIDRQAYRVRISQQGIEVTANGDAGLLYGAATVAQLLRRDNAGRLTVPEGQLEDWPQ
                     YQLRFLHWDTKHHQDRMETLKRYLDWSARMKINMIGFELEDKFSYPSHPVIGAPGAFT
                     PEQLQEIVDYGLERFIQVVPVIQSPAHMAYVLKHPEFADLRDDGNNYQIAMCDARAYP
                     LIFSMYEDVIKATRGVDYFFVSTDEMYYAGIGKNCAKPYNEENRSLQWVNFAQAAEKF
                     LSARNRRMLAWVEYPLLPEHAKLLPRGIIDGVVGEEEYLPAEKELGIRQLAYNSMQGA
                     TYLFPNPLATGGGRGGEGQLAQAFHSILTGRHTRGNPIGVFGAAWDDSGLHNETFWLG
                     WSAIAQYAWTPGTPSVDQHIAEFMNLYYGPGVTNMFDIYRTLQTQSRSWERSWDREVS
                     KVRGPGYGNSYGKGRGTERHDLTLTPPAIPALPDLAAAPVYAKQYAGRVAEARANLLE
                     NDRLAASLQENVGRASRNRYNLEVFLALTRFTGAHWHLLTALAGAEQSFEQARREHEA
                     KRPARAVGHMVAAYNRIGAARKRSAEVFEGLQAVYQKSRYPKGQSVGGRKFVHVLDDT
                     KDHWADRTADLTFMIAPEKSIGLDEWQKKLNAVIHEYAKTHNVPVRGLAEARLEE"
     gene            complement(2590..>2877)
                     /locus_tag="VFQ79_14690"
     CDS             complement(2590..>2877)
                     /locus_tag="VFQ79_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU0140968.1"
                     /translation="DDLLSADEMRRAKDHLKGSLMLSLESSSSRMANLARQELFFGRF
                     FSMDELAGRIEQVTPDEVQQIAREFFDPNYIALTMLGRLDEVNVDRSNLVC"
BASE COUNT          573 a          839 c          858 g          607 t
ORIGIN      
        1 tgctcactga gtgagcgttg cgcgtaggtg tatgtcgagc cggcggccgg aaatgcaccg
       61 gccatcttgc catagctcga tgcggtgaac agcatcgcca ccaacgcgac caggtagctt
      121 aaggcagcgt gtccatgtga gacctgttgg acgatcccga acacgggata ggccgcggtg
      181 ggcgtcaaaa tgacgaggcc gtagagaaca aggtcgcccc gtgaaagaac acgccggaat
      241 tgcggtttgt agcccagcgt atccagcgca gggtctgaca tgcgatcggc cattactctt
      301 cgagccgtgc ctctgccagg cctcttacag gaacgttgtg ggttttcgcg tattcatgaa
      361 taacggcatt cagcttcttt tgccattcgt ccagcccgat gctcttctcc ggagcaatca
      421 tgaatgtcag atcggcagtg cggtcggccc aatgatcctt cgtgtcgtcg agaacgtgaa
      481 cgaacttacg gccgcccacg gactgtcctt ttggataccg gcttttctgg taaacggcct
      541 gcagtccttc gaaaacttcc gccgagcgct tgcgcgccgc gccgatccgg ttataagccg
      601 ccaccatgtg acccaccgcc cttgcgggcc gcttcgcttc gtgttcgcgg cgggcttgct
      661 cgaaggattg ctcggctccc gccagcgcgg tcaacaggtg ccagtgcgcg ccggtgaaac
      721 gggtcagcgc cagaaagact tcgagattgt aacggttccg gctggcacgc ccgacattct
      781 cctgaagcga agccgccagg cggtcgtttt caagcaggtt cgccctcgcc tccgccactc
      841 gcccggcgta ctgcttcgca tagacgggag cggcagccag gtccggcagc gcgggaatcg
      901 caggcggagt gagcgtcagg tcgtgccgtt ccgtcccgcg gcctttcccg taagagttgc
      961 catatccggg gccgcgaacc ttcgacacct cccggtccca ggagcgttcc caactgcgcg
     1021 actgtgtctg gagcgtccga tagatatcga acatgttcgt gactccggga ccatagtaga
     1081 gattcataaa ttccgcaatg tgctggtcca cggaaggagt gccgggagtc caggcatact
     1141 gcgcgatagc ggaccacccg agccagaagg tttcgttatg aagtccgctg tcgtcccagg
     1201 ccgctccgaa cacgccgatc ggattgccac gcgtgtgcct tccggtgagg atggaatgaa
     1261 aggcttgcgc gagttgacct tcgcctccac gcccccctcc ggtcgcgagc gggtttggaa
     1321 acaggtatgt cgcaccttgc atggagttgt atgccagttg gcggatgccc agctcctttt
     1381 cggccgggag atattcttcc tctcccacca ccccgtcaat aattcctcgc ggcagcagtt
     1441 ttgcgtgttc tggaaggagg ggatactcca cccaggccag cattcgcctg ttgcgggcag
     1501 acagaaactt ctccgccgct tgcgcgaagt tgacccattg caagctccgg ttttcttcgt
     1561 tgtacggttt ggcgcagttc tttccaatgc cggcgtaata catctcgtca gttgacacga
     1621 agaagtaatc gacaccgcgt gtcgccttga tgacgtcctc gtacatggaa aagatcaggg
     1681 gataagcgcg cgcgtcgcac atcgctatct ggtagttgtt gccgtcgtcg cgaagatccg
     1741 cgaattccgg atgtttcagc acataggcca tgtgagcggg cgattggatc accggtacga
     1801 cctgaatgaa ccgctccagg ccgtaatcga cgatctcctg aagttgctcc ggagtaaatg
     1861 cgccaggcgc tccaatcact ggatgtgaag gataagagaa cttatcctcc aattcgaacc
     1921 cgatcatgtt tatcttcatg cgggcagacc agtccaggta gcgcttgagt gtttccatgc
     1981 ggtcctggtg atgcttggtg tcccagtgaa ggaaacggag ctgatattgg ggccagtcct
     2041 ccaattgccc ctcgggtacg gtcagacgcc cggcattgtc gcggcgcaaa agctgagcca
     2101 cggtggccgc tccgtacagg agtcccgcat cgccgttggc ggtcacttcg atgccttgct
     2161 ggctgatacg gacgcggtag gcctggcggt cgatctcggg ctcgcggccg gtcttcaccg
     2221 cgcccgcctg aatcgcaagc cgtataacga cgccgcccga tggcggatgg aaacgaagtc
     2281 cgtggaaatc ctgaagatcg cggatcagcg ctcgcacagc gatgtgctgg cttccaacgg
     2341 ccccggcatc gattcgccaa ctcgagtcga caacgacgtc gcgcgtgtca agctgaacct
     2401 tccggggagt tggaatcacg gagtaaccac gaagccacaa atctgttaag ggctgcgccg
     2461 gcagagcggt ggcgcaaaga agacaaagaa ccagacgatc gaacattttt ttatgtgaag
     2521 tacttcagac gatcatatcg tgatggagaa cgcggatgcc aattggagga cctggcgggg
     2581 aagcgcgctt cagcagacaa ggttgctgcg atcgacgttg acttcgtcca gccgtccgag
     2641 catggtaagg gcgatgtagt tggggtcgaa gaactcccgg gcgatttgct gtacttcgtc
     2701 cggtgtgacc tgttcgattc ttccggccag ttcatccatg ctgaagaatc gtccgaaaaa
     2761 aagctcctgc cgcgccaggt tcgccatgcg gctggaactg gattcgagac tgagcatcag
     2821 cgaacccttc agatgatctt tggcgcgccg catttcgtcc gcggaaagca ggtcgtc
//
LOCUS       DASTMK010000338        11248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_529018, whole genome shotgun sequence.
ACCESSION   DASTMK010000338 DASTMK010000000
VERSION     DASTMK010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529018"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFQ79_14695"
     CDS             <1..798
                     /locus_tag="VFQ79_14695"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0140969.1"
                     /translation="PLRPFLKTLWAMDQSSPPRAADRERVLPTGTMHLAVRLSDHPLR
                     LFNDVYDIAGHTIGDAVVGGARTTAYLRDVSRPSRSVGAQLHAGACEILFGVPAGELA
                     ERHTQLDAIWGRPALEMRERLLEAGSLEQQLDLFESILAGRLPRLLGLHPAVAHALER
                     LPAMTDVSEVVRETGYSHRRFIALFRHAVGLTPKVYCRVLRFGQALERVSTDPAAPHA
                     GLALEAGFSDQAHFNREFREFAGLTPGEYRRDPPLLTYHVPIRRPFR"
     gene            875..1315
                     /locus_tag="VFQ79_14700"
     CDS             875..1315
                     /locus_tag="VFQ79_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0140970.1"
                     /translation="MKIHELFAYLHVQEAAKAIEFYSRAFGAQEKFRLTEPGGRIGHA
                     ELDFGGTMVMLADEFPECNIRGPLAIGATSVTIHLHVDDADEVIRRAVEAGAVLEREP
                     KDEFYGERSGCIRDPFGHRWTIGHSIENISTDEMQRRYTEILTK"
     gene            complement(1329..2270)
                     /locus_tag="VFQ79_14705"
     CDS             complement(1329..2270)
                     /locus_tag="VFQ79_14705"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU0140971.1"
                     /translation="MANIFISYRREDSAGYAGRLCDRLNAALGSDRVFMDVEDIQPGQ
                     DFAAAIGEKIAGCDIVLAVIGPQWLPSLRKRLGQGEDFVHEEISAALRRGITVVPVLV
                     GGAVMPAAQELPESLAPLHRRQAIEIRDADFDDGVKRMTKFLEVSPVPKRRYQWMAAA
                     AVAVVVLVAILFFVRRPAPPDVQRPVPLDVQHPVPPDISRPAPANVRLPAPDIEGVWI
                     ARMQKEGQRPYSIRFEFAVSGSSLNGLVEYPTGQGAIREGRIEGRRLTFHTVHVPQFA
                     SEPATIRFDGEVAEDAIRLRIFDDSGIAKGVAKRVAK"
     gene            2380..3534
                     /locus_tag="VFQ79_14710"
     CDS             2380..3534
                     /locus_tag="VFQ79_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140972.1"
                     /translation="MALLHVFGTTLVEQDELDEKLRTAGDAIRTFGAQVTNARVPDQV
                     EEDVAIEFRDGTVTAREFLAGPQLNATTPLNPVGIGRPASIILRHLYTGGFPKGSVFG
                     SVRKDMLVTSAVRDTFATFNMAPRAINYTKRHITVQSHIKGPAATEDGTPLVYYSPAV
                     TAMSTNATFEMSFDDFPAELVGKIGNAITSAGGIPVFGPYSGVLIGVGAAIKLVSSLA
                     SDLVDSRPEFTETERLEFEVPGSVVPTAGFKVVCSDPSEFREYEFMMDAGLVHKVSGK
                     VYSGNQPYVVFLLDGTEQPTLAGFTPTAASAALLSRFLSQKEGSEVIIDALVESVKLY
                     NDTRFRNEADRLQREIDKLPENSELRLQMEKRREAFVANIISDLLKPARA"
     gene            3589..4866
                     /locus_tag="VFQ79_14715"
     CDS             3589..4866
                     /locus_tag="VFQ79_14715"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU0140973.1"
                     /translation="MMRLKLGSAVIGLIAAALLLLVWPERMLESEPNHASPVESAELA
                     NPPEIEYHTESVPIRSGDTLEGLLLNAGIDRLSTGEVISAVRGTFDVRAFRAGAELKL
                     TRATTGSIHSLEYSIDPDRTLKLSSGDSGFAATIEEVPGTIRNAPICGTLEGSLFESI
                     ERMGERPELAIQLAEIFAWDLDFYTDPQEGDRFCILVEKKDYANGQSSTYRRILAASY
                     ENASETFEAFAFEDEQGKPQFYARDGRALRAAFLKSPIKFDVRISSRFSHRRFHPVLK
                     RYRPHLGTDYAAPTGTPVQAIASGRVVFSGYSRGAGNLVRVEHTGGYETYYMHLSRRY
                     VRTGQRVEQGQRIGAVGATGLATGPHLDFRFRKQGKFVDFERLRLPRESKLSAARIGA
                     FTTVRDQYDGLIRAASGRALATVARAGSPAGTP"
     gene            complement(4890..5672)
                     /locus_tag="VFQ79_14720"
     CDS             complement(4890..5672)
                     /locus_tag="VFQ79_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0140974.1"
                     /translation="MIRFALLVAGATACALAQIVPHNSSGVSMGHIHIVTPQPELQKK
                     IWVDVLGGKLVERGPLEYAMFPGVFVGFRKGESGGGTEGSVIDHLGFGVQDLAATKAK
                     LTAAGVRIQREMPATKQLFAMFPDDVKVEFSELPGIAHPVVHHHVHFASNQVDAMRQW
                     YADTFGAVPGTRGKFQTADLPGVNLSWNPADKPTLPSKGRAVDHIGFEVGDIQAFCKK
                     LEAAGIKLDMQPTRRDDLGLIIAFVTDPWGTRIELTQGLASR"
     gene            complement(5739..7118)
                     /locus_tag="VFQ79_14725"
     CDS             complement(5739..7118)
                     /locus_tag="VFQ79_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0140975.1"
                     /translation="MKRLIWAASMITGLCLASTIPTSAPTPAGSDAGAWRTWVLASGS
                     ELRLPPPAGTASTEGELRWLKEFMTRRDEQAARQIRFWDAGAPPYRWIEMLSDRVRSG
                     RLPVSPLSFRAYALLSVAMYDATAAAWDSKYTYNRPRPSEADPAIRPLVRTPDSPSYP
                     SEHAVVSAAAAEVLAYLFPNEAANFRDLADEAARSRLFAGVQYPSDVTAGLDLGRAVG
                     RKVVESARMDRTDAAWTGTAQSGPGMWTGTNPGFVTAPLWRPWFLASASEFRPAPPPA
                     YDSPARAAEIAELKNFARTFNSNASAFFWQTGEGVHTWFFSTAGQKMFETGADRNPPC
                     AARAYALVAIAQFDAMIASNDGKYTYWTIRPAQQDTSLTTVFATPNFPSYPSNHSILS
                     TTRAEILAYLFPDDAAYFRMRGEEAGLSRLAAGIHFRSDHEAGSDMGRKIARKLIDLA
                     EQDGSSCQE"
     gene            7229..10315
                     /locus_tag="VFQ79_14730"
     CDS             7229..10315
                     /locus_tag="VFQ79_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0140976.1"
                     /translation="MSRQSRTTIIRFGGYELNVRAGELRRDNGTPARIQDKPLQLLLL
                     LLERPGELVTREEIRERVWGPSRYYDFEDSLNQAVRKLRGVLGESASNPRFIETIPRR
                     GYRLISPVEEHRRPDGGSNGMGGAREGAVGRQRELSELHLEAAAAAEGSGRVVCISGE
                     PGIGKTTLAECLCEQLSAERDTRIVIGRCSERLAGTEAYLPVLEALENSIVGDDCAAC
                     AELLKRTAPSWFLQLAHALSGIDTGEPPAVDAWTASQERRKREFLTYVDASSRERPLV
                     LFIEDLHWADVSTVDLLSYVAARCAAMPLLILGTYRPSDLLLSRHPFLDVRLELQTRR
                     LFREIELTPLGQDDVKTYVDLKFPGNRFPANFNPSIYQRTEGSPLFMADLLRDMRNTG
                     IIAVEEGEWVLTRPLAEIQNAIPDSVRGMVERKIGKLSPDHRRLLETASVQGYDFQSS
                     VLARVSALDGIAVEETLDYLERKHRLVRLIKEESFPDSTFTLHYTFSHALYQEFLYDS
                     LHASRKAALSAAVAEELIALHRGGVEIASQLGPLFETARDRNRAAHFFAIAAANAGKL
                     SANRQAVGLATRALENARQLEGDIRERRLLEAGLRLAEARLALSEFERSIKHFEFAAS
                     AAERLGDVESQVDALCGAAISCGYVKDMDGMRERGSKALEAARAAGTTPAFAQSVLGF
                     ERMFAGDLGIARHYLDQALPALRNHGPVHAAVFASGSRALLDHFQSEYRDADEVLDWA
                     MEQLRRHGSCSDLLRNLWVRGMVLGNQGRVSAALDTLMEGMRLADLNKEAFWFSRLPN
                     TIGWIYSEILDFESGLRFNEEGVRTGQSSGTPETEGNSHINLASIYLALHDHGRAREH
                     LDEGDRLLNEHRHKRWLRWRFNIRLDLEFAGYELALEDAERARRHAGQALSRASEAGA
                     RKHAAAAHKLLGDVCALEENFAGSAWQYDAALAILDRNPCPLIDWKISRSAASVAAYA
                     GDPKRASRLHKRFEKTAALVGGSIRDDKLRRRFLESAGVKGMAPASRASMARGTPA"
     gene            complement(10278..>11248)
                     /locus_tag="VFQ79_14735"
     CDS             complement(10278..>11248)
                     /locus_tag="VFQ79_14735"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140977.1"
                     /translation="PDHWHAAPTIAAFEAGKDVYVEKPLATTITEGRAMRDAAARYKR
                     IGQMGTQWRSGEHYREAVEMVQSGKIGKVRQVRCTAYLDWVKECGNPPDGPAPAGVDY
                     DMWLGPAPSRPFNKNRFHFNFRWFWDYAGGLMTDWGVHLINIALWGMGPEWPKSVVSS
                     GGKYVLRDNTETPDTQITVYDFPSYTLIWEHQVGCGLGPDRREHGVFFTGTDATLIVD
                     STGWEIIAEPKRRTSVVEMKRAALVDEKVRANHARNFLDCVRTRKAPVENLEVGHHVS
                     TVAHLGNIALRSHSRIEWDPKTETVPGNAAANALVTREYRAPWKLG"
BASE COUNT         2164 a         3355 c         3498 g         2231 t
ORIGIN      
        1 cccttgcggc ctttcctaaa gaccttgtgg gcgatggatc agtcgagtcc tccccgcgcg
       61 gcggaccggg aacgggtctt gcccactgga acgatgcacc tcgctgttcg tctctcggat
      121 caccctctgc ggcttttcaa cgacgtatac gacatcgccg gccataccat cggcgacgcg
      181 gtggttggcg gagcacgcac cacagcttat ctgcgggacg tctccaggcc ttcccgttct
      241 gttggcgctc aacttcacgc gggcgcgtgc gaaatcctgt tcggtgtgcc agccggcgaa
      301 ctggcggaac gtcatactca gcttgatgcg atctggggac gcccggcttt ggaaatgcgt
      361 gaacggctcc tggaggccgg ctccctggaa caacagcttg atctgttcga atccattctc
      421 gccggacggc ttcccagatt gctgggactc cacccggccg ttgctcatgc gttggagcgc
      481 ctcccggcaa tgactgatgt cagcgaggtg gtgcgagaga ccggctacag tcaccggcga
      541 ttcatcgcgc tgttccgtca tgccgtcggg ttaactccaa aagtctactg ccgggtgttg
      601 cggttcgggc aggcgctgga gcgggtttcc acggaccccg ccgcgccgca cgctggattg
      661 gcgctggagg cgggcttcag cgaccaggcg cacttcaatc gggagttccg cgaatttgcc
      721 ggactcacac cgggtgagta ccgcagagat ccgccgctgc tgacgtatca cgttcccatc
      781 cggcggcctt tccgctgagc atgttcgggt tcaattttgt tcaagacgag tcgggcgttg
      841 catcgtacag tcgtggaaga ggggtaaatc aaccatgaag atacacgagc ttttcgccta
      901 cttacacgtg caggaagccg caaaggccat cgaattctat tccagggcct ttggagcaca
      961 ggagaaattt cgcctcaccg agccgggcgg gcgaatcggt cacgccgaac tcgacttcgg
     1021 cggaaccatg gtgatgcttg ccgacgaatt ccccgagtgc aacattcgcg gaccactcgc
     1081 catcggcgcc acttctgtta ccatccatct tcacgtcgac gacgccgacg aggtgattcg
     1141 gagggctgtt gaagccggag ccgtgctcga aagggagccg aaggatgagt tctacgggga
     1201 gcggtcgggc tgcattcgcg atccgttcgg ccaccggtgg accatcgggc acagcatcga
     1261 gaatatatcg acggacgaaa tgcagcggcg atatacggaa attctcacga agtgacagca
     1321 tcgcgtcatc atttcgcgac tcgttttgcc acgcctttgg cgatgccgct gtcgtcgaag
     1381 atgcgtagcc ggattgcatc ctccgccact tccccgtcga agcggatcgt ggcgggttcg
     1441 gaggcgaatt gcggcacgtg cacggtatgg aaagtcagcc tgcggccttc gatgcggcct
     1501 tcgcggatgg cgccttgtcc cgtcgggtat tcgacaaggc cgttcaggct gcttccactc
     1561 actgcaaatt cgaaccggat cgagtaaggg cgctgcccct ctttctgcat tcgggcgatc
     1621 cagactccct cgatgtcggg cgcgggaagc cggacattcg ccggagcagg acggctgata
     1681 tccggaggaa caggatgctg gacatccaac ggaacaggac gctggacatc aggcggagca
     1741 ggccgccgga caaaaaaaag aatcgcgacc aggacgacga cggcgaccgc agccgcggcc
     1801 atccactgat agcggcgctt gggtaccggt gacacttcga ggaacttcgt catccgcttc
     1861 acgccatcgt caaaatcggc atcgcggatt tcgatcgcct gacgccgatg caggggcgcc
     1921 aggctttccg gcagttcctg tgcggcgggc atgaccgcgc cgcccaccag cacgggaacc
     1981 acagtaatac cgcgccgcag agccgcggag atctcctcgt ggacaaaatc ctcgccctgg
     2041 ccgagacgtt tccgaaggct cggcagccac tgcgggccga tgaccgcgag cacgatgtca
     2101 catccggcaa ttttttcgcc tattgccgcc gcgaaatcct ggccgggctg tatgtcctcg
     2161 acatccatga acacgcgatc gctgccgagg gcggcgttca gacggtcaca gagacggccg
     2221 gcgtagcctg cgctgtcctc cctccggtag ctgatgaaga tgtttgccat tgaggtgcgg
     2281 ccctccggat gccgaccgtc atcatgatat tccgaattac acacctgtgg cacggaaacg
     2341 tcacgccgcg tatactgcgt tagaacccta aggagggcta tggcactctt gcatgtcttc
     2401 ggcacgaccc tcgttgaaca ggacgagttg gatgagaaac tccgcacggc gggcgatgcg
     2461 attcgcacct ttggcgcaca ggtgacgaat gcccgcgtcc ctgaccaggt ggaagaagat
     2521 gtcgccatcg aatttcgcga tggtacggtg acggctcggg agtttctcgc cggtccgcaa
     2581 ctcaacgcca cgacgccgct gaatccggtc ggtatcgggc ggccggcttc catcatcctc
     2641 cgccatctgt acacgggcgg ctttccaaaa ggatcggtat tcggcagcgt ccggaaagac
     2701 atgctggtca cgtccgccgt tcgcgatacc tttgcgacct tcaacatggc gcctcgagcc
     2761 atcaactaca ccaagcggca tattacggtg cagtcgcaca tcaagggtcc cgccgcaacc
     2821 gaggatggga caccgctggt ctattacagc cccgcagtca ctgcgatgtc gaccaatgcg
     2881 acatttgaaa tgtcttttga cgatttccct gccgagttgg ttggtaagat cggcaacgcc
     2941 atcacgagcg ctggtggaat tccggtcttc gggccttata gcggcgtgct gatcggagtg
     3001 ggtgcggcga tcaagcttgt atcttcgcta gcctccgatc ttgtcgacag ccgcccggag
     3061 ttcacggaaa cggaacggct ggaattcgag gtgcctggta gtgtcgttcc taccgccggc
     3121 tttaaagtgg tctgttccga tccatccgag ttcagggaat acgaattcat gatggacgcc
     3181 ggactcgttc acaaggtgag cgggaaagtc tattccggca accagccgta cgtcgtgttt
     3241 ctgctggatg gcaccgagca accgacgctc gcgggattca ctccaacggc tgccagcgcc
     3301 gcactgcttt cgcgatttct ctcacagaag gaaggctcag aggtcattat cgacgcgctg
     3361 gtggaatccg tgaagttgta caatgacacg cggttccgta acgaagccga ccggctgcag
     3421 cgcgagatcg acaagcttcc ggagaactcg gaactgcggc tgcaaatgga gaagcgtcgc
     3481 gaagcttttg tcgcgaacat tatttccgac ctcctgaagc ctgcccgcgc gtagcaggct
     3541 gttttgacgt atcgcaccgg cagttcgtaa agtgaaacaa tgagccgcat gatgcgactg
     3601 aaactgggat cggctgtcat cggcctgatt gcggccgctt tgctgctgct tgtctggccg
     3661 gagcggatgc tggagagtga accgaaccat gcctccccgg tggaatccgc cgaacttgcc
     3721 aatccccctg agattgaata tcacaccgaa agcgttccca tacgatcggg cgacacactc
     3781 gagggccttc tgctcaacgc gggaatcgac cggctttcga ccggcgaagt gatttccgcc
     3841 gtccggggaa cgttcgatgt ccgggcattt cgcgcgggcg cggaactgaa actgacccgc
     3901 gcgacgacgg ggagcattca ctcgctcgag tattccatcg atcccgatcg cacgctcaag
     3961 ctgtcttcgg gggattcagg ctttgcggcc acgattgaag aggtgccggg gacgattcgc
     4021 aacgcgccca tctgcggaac gctggaagga tcgctgtttg agagcatcga acggatggga
     4081 gaacgtccgg agctggccat tcaattggct gagatctttg cctgggatct ggatttttat
     4141 acggacccgc aggaaggcga ccggttctgc attctcgttg agaagaaaga ttacgcgaac
     4201 gggcagtcat ccacttacag aagaattctg gctgcatcgt acgagaacgc gagcgaaacc
     4261 ttcgaggctt ttgcgtttga ggatgagcag gggaaaccgc agttttacgc gcgcgacggg
     4321 cgggctcttc gggccgcgtt tctgaaatcg ccgatcaagt tcgacgtgcg gatcagctcg
     4381 cgattttcac accggcgttt tcatccggtg ttgaaacgct accggcccca tttgggcacg
     4441 gattacgcgg cacccaccgg cacaccggtg caggccatcg cttcaggccg cgtggtcttt
     4501 tcgggttact cgcgaggcgc cgggaatctg gtccgcgtcg agcacacggg cggctacgag
     4561 acgtactaca tgcatctttc gcgaaggtac gtgcggaccg gacagcgcgt ggaacaaggg
     4621 cagcgcatcg gcgccgtggg ggccaccggc cttgccacgg gtcctcacct ggatttccgt
     4681 ttccgtaagc aggggaaatt cgtcgatttt gagcggctgc ggcttccgcg ggaatcgaag
     4741 ttgagcgcgg cgcgcatcgg ggcctttaca acagttcgcg atcaatacga cggcttgata
     4801 cgagcggcct ccggacgggc actcgccacg gtagcacgcg ccggatctcc cgccgggact
     4861 ccatagtttc gtccgaaccg acccggcggc tagcggctcg ccaggccttg cgtcagctcg
     4921 atgcgggttc cccagggatc tgtcacgaac gcgatgatca ggcccagatc gtcgcggcgt
     4981 gttggctgca tgtcgagctt gatgcccgcc gcttccagtt tcttgcagaa cgcctggata
     5041 tcaccgactt cgaacccgat gtggtccact gcccgccctt tggacggcag ggttggtttg
     5101 tccgcggggt tccaggatag attgacgccg ggcagatcgg ctgtctggaa cttgcctcgc
     5161 gtgcccggca cagcgccgaa cgtatcggca taccactggc gcatcgcgtc cacctggttg
     5221 ctggcgaaat gtacgtgatg atggaccacc gggtgggcga tgccgggcaa ctccgagaac
     5281 tccactttca cgtcgtcggg aaacatggcg aacagttgtt tcgtagccgg catttcgcgc
     5341 tggatgcgta cgccggcggc cgtaagcttc gctttggtcg cggcgagatc ctgcacgccg
     5401 aatcccaggt gatcaatcac cgagccttcg gtgcctccgc cggactcccc cttccggaat
     5461 ccgacaaaaa cgccggggaa catcgcgtat tcgagcggcc cacgttccac cagcttgccg
     5521 cccagtacgt caacccagat ttttttctgc aattccggtt ggggtgtaac gatgtggatg
     5581 tgtcccatcg agactcccga gctgttgtgc ggaacgattt gtgcgagtgc gcaagccgtg
     5641 gcaccggcaa cgagtaaagc gaatcgtatc atgccctgag attatgccat acctccgagg
     5701 agccgtccgt atccctgcgt cagggcggag gcagagtgtc attcctggca cgacgagccg
     5761 tcctgttcgg ccagatcgat cagctttctc gcaatctttc tccccatgtc cgaaccggct
     5821 tcgtgatcgc tccggaagtg aattcccgca gccagacgcg acagcccggc ttcctcgccg
     5881 cgcatacgga aatacgcggc gtcatccggg aaaaggtagg caaggatttc cgctctggtg
     5941 gtggagagga tagagtggtt ggaaggataa ctcggaaagt tcggggtggc gaaaactgtg
     6001 gtcaatgatg tgtcctgctg cgcgggacgg atagtccagt acgtgtattt cccgtcgtta
     6061 gaggcgatca tggcatcgaa ttgcgcaatc gcaaccaacg cgtaagcgcg ggccgcgcag
     6121 ggtggattcc ggtccgctcc cgtctcgaac atcttctgcc ccgcagtgct aaagaaccat
     6181 gtgtgaactc cttcgcctgt ctgccagaag aacgcggatg cgtttgagtt aaatgttcgc
     6241 gcgaagttct tcaactcggc gatttcggca gcgcgcgcag gcgaatcgta agcgggagga
     6301 ggcgcggggc ggaactcgga agccgaagca agaaaccagg gccgccacaa cggggcagtg
     6361 acaaatccgg ggttagtgcc cgtccacatt cccgggcccg actgcgccgt gcccgtccac
     6421 gcggcatccg tgcgatccat tcgcgcggat tcgactacct tccggcctac ggcgcgtccc
     6481 aagtcgaggc ccgcggtcac gtcgctcgga tattggactc cggcaaacag ccgcgatcgt
     6541 gcggcttcat cggccaaatc ccggaagttc gcggcctcat tcgggaacag gtatgccagc
     6601 acctcagcgg cagcggctga aaccacggca tgttcgctgg gataagaggg actgtccggc
     6661 gtgcgcacca gtggccggat ggcagggtcg gcctcgcttg gccgcgggcg attgtacgtg
     6721 tacttcgagt cccaggcggc tgcggttgcg tcatacatgg caacgctcaa cagcgcatag
     6781 gcgcgaaaag agagtggact gaccggcagc cttccgctgc gaacgcgatc cgacagcatc
     6841 tctatccatc gatagggcgg agcgccggca tcccagaacc ggatctgccg cgccgcctgc
     6901 tcatcacggc gtgtcatgaa ttctttaagc catcgcagtt cgccctcggt ggaggcagtg
     6961 ccggcgggag gcggcaaccg cagctcgctt cccgaagcta gtacccaggt tcgccaagct
     7021 cccgcatcgg aaccggcggg tgttggcgca gaagttggaa tcgttgaggc gagacacagc
     7081 cccgtaatca tgcttgcagc ccagatcaga cgtttcataa attgtccttt gatcgagcat
     7141 gaacccgaac cgggcgcacc accatgaaca attccgaatc agattctgat ccggcggtgc
     7201 tataatcctg accacggggg gctgggccgt gagccggcag agccggacaa caatcattcg
     7261 attcggcggc tatgagttga acgtccgtgc gggcgagttg cgccgcgata atggaacgcc
     7321 ggcccggatt caagacaaac ccctgcaatt gctgctcctg cttctcgaaa ggccgggcga
     7381 actcgttacg cgggaagaga ttcgcgagcg cgtgtggggc ccgagccgct actacgattt
     7441 cgaggacagc ctgaatcaag ccgtgcgcaa gctgcggggc gttctgggag aaagcgcttc
     7501 caatccgcgt ttcatcgaaa caattccgcg gcggggatat cgccttattt cgccggtcga
     7561 agagcaccgg cgtcccgatg gcggcagcaa tgggatgggg ggcgcgagag agggagccgt
     7621 cggacggcag cgcgagttgt ccgagctgca cctggaagca gccgcggcgg cggagggtag
     7681 cggccgggtg gtttgcatct cgggcgaacc cggaatcggc aagacgacgc tggccgaatg
     7741 cctctgcgaa caactctccg ccgaacgcga cacccgcatc gttatcggcc ggtgttcgga
     7801 gcgactcgcc ggcacggaag cgtatctccc cgttttggag gcactcgaga attcgatagt
     7861 cggcgatgac tgcgccgcgt gcgcggagct attgaagaga actgcgccga gctggttctt
     7921 acaactggca cacgcgctgt ccggtatcga tacgggcgag ccgccggcgg tggacgcgtg
     7981 gaccgcttcg caggagcgaa ggaagcgtga gtttcttacc tatgtcgatg cgtcgtcccg
     8041 ggaacgcccg ctggtgctct tcatcgagga tctgcattgg gccgatgtct ctacggtcga
     8101 tcttctttcg tacgttgcag cgcgttgcgc cgcgatgccg ctgttgattc tggggacata
     8161 ccggccctcc gacctccttc tgagtcgtca tcctttcctg gatgtcagac tggaattgca
     8221 gacccgcagg ctatttcgcg agatcgaatt gacgccgctt ggacaggatg atgtcaagac
     8281 ctacgtcgat ctgaaatttc caggcaaccg gtttccggca aacttcaacc cttctattta
     8341 tcagcggacg gaaggcagtc cgcttttcat ggccgatctg ctgcgagata tgcggaacac
     8401 cggaatcatc gcggtggaag aaggcgagtg ggtgctgacg cggcccttag ccgagatcca
     8461 gaacgccatt ccggattccg ttcgcggcat ggtcgaacgc aagatcggaa agctctctcc
     8521 ggaccaccgg cgcctgctcg agacagccag tgtacaaggc tacgactttc agtcttcggt
     8581 attggcgcgt gtgtcagccc tggatggcat cgctgtcgag gaaacgctgg actaccttga
     8641 acggaagcac cgtctggtcc ggttgataaa agaggagagc tttccggatt cgactttcac
     8701 tttgcactac actttctcgc atgcgctgta ccaggaattc ctttacgatt cactgcatgc
     8761 gagccggaaa gccgctctga gcgcggcggt agcggaggag ctgatcgcac tgcatcgtgg
     8821 cggcgtggaa atcgcttcgc aactcggccc gctgtttgag acagcgcgcg atcggaaccg
     8881 tgccgcgcac ttctttgcca ttgccgccgc gaatgctgga aaactatcgg ccaacaggca
     8941 ggcagttggt cttgcaacgc gcgccctcga aaacgcccgg caactggaag gagacattcg
     9001 cgaacggcgg ctgctggaag ccggtctcag gctggccgaa gctcgcctgg cgctctcgga
     9061 attcgagagg tccatcaagc atttcgagtt cgcagcctcg gcggcggagc ggctgggcga
     9121 cgtcgaatcg caagtggacg cgctttgcgg ggcggcgatc tcttgcggct acgtgaaaga
     9181 catggatgga atgcgcgagc gcggaagcaa ggcgctcgaa gcggccagag ccgccggcac
     9241 gaccccggcc ttcgcgcaga gcgtgctggg attcgaacgg atgttcgcgg gcgatctggg
     9301 cattgcacgc cactaccttg accaggcgct tcctgccttg cgcaatcacg gaccggttca
     9361 tgccgccgtt ttcgcttccg gcagccgcgc tctgctcgat cacttccaat ccgaataccg
     9421 ggatgcggat gaggtgctcg actgggcgat ggagcagttg cgccggcacg gctcctgctc
     9481 cgatctgttg cgcaacctgt gggtgcgcgg aatggttcta ggcaaccaag gccgggtgtc
     9541 ggcggcgctc gacaccctga tggaaggaat gcggctcgcc gatttgaata aagaggcctt
     9601 ctggttttcg aggctgccaa acacgatcgg ctggatctac agcgagattc tggacttcga
     9661 atccggattg cgtttcaacg aggaaggcgt ccggactggg cagagttcgg gaacgcccga
     9721 aacggaaggt aattcgcaca tcaacctggc gagcatctat ctggccttgc acgatcatgg
     9781 gcgcgcccgt gaacaccttg acgaaggcga ccgactcctg aacgagcacc ggcataagcg
     9841 ctggctgcgg tggcgcttca atatccgcct ggatctggag ttcgccggct acgagttggc
     9901 tctggaagat gcggaacggg cgcgaaggca cgccgggcag gcgctgtcgc gcgcatccga
     9961 agcgggcgcc aggaagcacg cggcagcggc tcacaagctg ttgggcgacg tttgtgcatt
    10021 ggaggagaac ttcgctggat ctgcctggca atatgacgcc gcactcgcga ttctcgaccg
    10081 caatccgtgc ccgctcatcg actggaagat ctctcgctcg gccgcgagtg tggcggcgta
    10141 cgcgggagat ccaaaacgcg cctctcgctt acacaaacgc ttcgagaaga ccgccgcatt
    10201 ggtcggcggc tctattcggg atgacaagct ccggcgaagg ttcctggagt ccgccggagt
    10261 aaaagggatg gctccggcta gccgagcttc catggcgcgc ggtactcccg cgtaaccagg
    10321 gcgttcgccg cggcgttgcc cggcaccgtt tccgttttgg ggtcccattc aatgcggcta
    10381 tgggagcgca acgcaatgtt acccagatgc gcaacggtgg aaacgtggtg gcctacctcc
    10441 aggttctcga ctggcgcttt gcgggttcgt acgcagtcga gaaagttgcg cgcgtgattc
    10501 gcgcgaacct tctcatccac taacgccgcc cgcttcatct ccactacgga ggttctgcgc
    10561 ttcggctcgg cgatgatttc ccaaccggtg gaatcgacaa tcaacgtggc atccgttccg
    10621 gtgaagaata cgccgtgttc gcgccggtcc ggacccaggc cgcaacccac ctggtgttcc
    10681 cagatcagcg tgtaggaggg gaaatcataa accgtgatct gcgtgtcggg ggtttcggtg
    10741 ttgtctcgca gaacatactt accgccggaa gaaacgacgc tcttcggcca ttcggggccc
    10801 atgccccaca aggcgatatt gatgaggtgc acgccccagt ccgtcatcag tcctccggcg
    10861 tagtcccaga accagcggaa attgaaatgg aaccggttct tgttgaacgg gcgcgacgga
    10921 gcgggtccga gccacatgtc gtaatcgacg cctgccggtg ccggcccatc cggcggattg
    10981 ccgcattcct tgacccaatc gaggtacgcg gtgcagcgca cctggcgcac tttgccgatc
    11041 ttgccggact gcaccatttc cacggcttcg cggtaatgct cgccgcttcg ccactgggtg
    11101 cccatctgtc cgatgcgttt gtacctggct gcggcatctc tcatggcacg gccctcggtg
    11161 atggtcgtcg ccaacggttt ttcaacgtag acgtccttgc cggcctcgaa ggcggcaatg
    11221 gtgggcgctg catgccagtg gtccggag
//
LOCUS       DASTMK010000339        16365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_112664, whole genome shotgun sequence.
ACCESSION   DASTMK010000339 DASTMK010000000
VERSION     DASTMK010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16365
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112664"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VFQ79_14740"
     CDS             complement(<1..1167)
                     /locus_tag="VFQ79_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEU0140978.1"
                     /translation="MRSAGSIRRVSYNKNIGPRPDSSRQNSILIRGARVHNLKNISLA
                     IPHNALTVVTGVSGSGKSSLVFDTIYAEGQRRYVESLSAYARQFLERMEKPAVDDIQG
                     IAPPIAIRQRNTTRNPRSTVGTATELYDFLRLLFARAGRTYCPQCGQRVERDTVDQVA
                     QRLLAEPEGSRWYVLFPVQVSHDAQALRDRLFDLRKKGFNRLYQPPGTYEFSSPESLL
                     EIDFDQPLFVLVDRLVIAPGIHQRIVDSIEICYRECGEVIFEQAVHEPVRRIRFSETF
                     ACKQCGIVFEDPEPRLFSFNNPFGACPRCQGFGNTIDYDMDLVVPDRWVSLKDGAVDP
                     WTKPQYAWYYDEFRRKARGKVRFDVPFAELTKQERQIVSDGVHDFFREVETKKYK"
     gene            1148..2131
                     /gene="mutY"
                     /locus_tag="VFQ79_14745"
     CDS             1148..2131
                     /gene="mutY"
                     /locus_tag="VFQ79_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU0140979.1"
                     /translation="MDPAERIRRKLLDWYRRARRPLPWRATSDPYRIWVSEVMLQQTR
                     VVAVIPYYERFIKRFPDVKSLAEAPEQELLACWSGLGYYSRARNLQRSAQMIVEMGGF
                     PENFKRIRSLPGIGDYTAAAIASIAFGLPHAALDGNVLRVLSRLSNDAGDIRARETGE
                     RISALASRLLDRKNPGDFNQAMMELGATLCRPKRPQCLLCPLASECNALAAGRQNELP
                     VKLRGAQTIQLEYTLLLVRRNGCFLLRQRNTASRKLAGFWEAPCTADLPAARVGREIG
                     RFKHSIMHHEYRVTVRFASVVKCPPGFRWIDASTLEQLPLTTATRKALKLL"
     gene            2203..3546
                     /locus_tag="VFQ79_14750"
     CDS             2203..3546
                     /locus_tag="VFQ79_14750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HEU0140980.1"
                     /translation="MKQRKRLLFAKSVAILSTIPALIYGFASGPLPNKSGAPGGGLCT
                     ECHVGSAAGTSIAVTAASGTTYTPGQAQQITVRITEAANVYGFQATARTSGNQQAGSF
                     TSGAGMKVISGEGFQFIEHNTPSSNSTFTFQWTPPANASGDITIYAAGNAANGNGDHT
                     GDHIHSGRLTLTAAGSQQPRPTIGQNGVLNGASFQPQIAAGTWVSVFGTNFASAERFL
                     LGSDLINGNTLPTELEGVRVEIDGRASPIGYLGPSQINVLAPDGTSSGPVRVEVRTAA
                     GTSEAAIVTSQAVAPAFFLYDPEGRKYIAAQHGVGNAPLGKAGLVAGSTPAAPGETII
                     LYATGLGPTDPAVPVGRIPSGAARIRENITVRFGETAATVDYAGVSTCCAGLYQINVV
                     VPATVQSGDVAVRAQIGSLQTQPDTFITIQAQQPQAQPQPQPQPEPETPYGETYY"
     gene            3613..4932
                     /locus_tag="VFQ79_14755"
     CDS             3613..4932
                     /locus_tag="VFQ79_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HEU0140981.1"
                     /translation="MLFGNSGSLPAAPNPVLKRTGAQVDGGLNCTACHQTYAPANSDN
                     RGRVVVESAYYKPGQKQIVRVRVEHPEAQRWGFQLTARTASDETKTAGSFTSSASVRV
                     ECDDGSLRGSLPPCAEGTLQFANHTMESTRNGTPGGVWWDIEWTPPATDVGDVVFYAA
                     GNAANGNGTNVGDRIYTTALRVGGCSTAVKPSIRGVVNAATYQPGVALNTIVSIFGAG
                     FAGAGQMRAAGIGDFDHGNFPKELACMAVEINGQRAPVVYVQGDQINAQVPTMPVSGP
                     VEMRVLANPGAPNQMASDPATIQMQNHAPALFTFGSSNSVAAVVPGTGQPIANPDVIQ
                     NGRPARPGEIIELYGTGFGVTEPVYQAGERPSEGARIRDPFTVTIGGTTLRAEDVIYG
                     GIAPTSISGLFQFNVRLPESMADGDLPVVIRIGGLETQNGVIIPVKR"
     gene            4966..5787
                     /locus_tag="VFQ79_14760"
     CDS             4966..5787
                     /locus_tag="VFQ79_14760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140982.1"
                     /translation="MKAAIPLLALFALSPMLQAAESGQRVAITYSSASVVNAASNVAV
                     LAPNTIASIYGEGLSWKERSISEDDIRSGNMPTILSQTGVRVIVNNIPACIYYVSPGQ
                     INFLVPANLLPGPATVRVTLDGAVGPKAEVELLPAAPAFFQLDAETIVATHADEAAVT
                     AENPARPGEIVVLYLTGLGRTKPWFRSGEIVTQAAWIEDRSKLTLLIDGEPVERSRIE
                     YAGVTPGSAGLYQINVKLPDSIRENPEIRVLMDEQASQEGLRLPVNPNPAPPEPE"
     gene            5839..6651
                     /locus_tag="VFQ79_14765"
     CDS             5839..6651
                     /locus_tag="VFQ79_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140983.1"
                     /translation="MRYLLAVVISGCCLAYGQEAGQEKESARWEEASQASTAGEYVVE
                     PGTRVPLNLINSISTKHAAEGDRVYLETAFPILTRGRIVIPPGSYVAGTVTQVKRPGR
                     LKGRGELYLRFDSLTLPNGVTRDFRARIGGLDGRASEELDRSEGKIKSEGNKGGDART
                     VGEATAAGASVGVIAGSTAGRAGMGAGIGAAAGATAGLVGVLLSRGPEAVLAKGTTVE
                     MVLDRPIHFTEEEVAFTQPPPRRSSSDGPGPAPSRKSRNLPMPGRYPLGWMF"
     gene            6807..8111
                     /locus_tag="VFQ79_14770"
     CDS             6807..8111
                     /locus_tag="VFQ79_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0140984.1"
                     /translation="MMQNRRSFLQGAAVAAVAGSASRILGANDRVNVAVVGLGGRGRA
                     HVTEWAKVPGSRIVALCDVDQAALERGQALAEKATGEKAKGYVEMRKLFEDKDIDAVS
                     MPLPNHWHALATIWACQAGKDVYIEKPACHNVWEGKKMVEAARKYNRMVQIGSQSRTV
                     PMKIRAMQLLKEGVIGKVYLAKGLCYKRRPSIGHKPDGPVPPGVNWDLFLGPAPMRPF
                     NENRFKYNWHWFWDTGNGDIGNQGVHEMDIARWGLGVAYPKSVVSFGGKYIYDDDQET
                     PNTQYASLDYGGMQIDFEVRGIFTDGTQAMPRQGRNTVGDLFYGADGYMSIDDDGFQV
                     YKGEKHEKVMDEKAERGVSSTMAHMDNFAKAVRSRNYKDLTADVELGVISADLCHLAN
                     IAYRVGRKLTFNAQASQFVNDAAASKLLTRDYRKPYVVPDKV"
     gene            complement(8130..9368)
                     /locus_tag="VFQ79_14775"
     CDS             complement(8130..9368)
                     /locus_tag="VFQ79_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140985.1"
                     /translation="MNRRTFLALSGSTVFAGASQSGPPKKIAAIITEYRPNSHADVIV
                     GKYLEGYNQDDQPPYPRSKIVSMFTEQVPKTDMSRERAKKYGVPIFRTVYDALTLGGS
                     NLAVDGVLLIGEHGDYPMNDKEQKLYPRFEMFLKITDVFRKYGRAVPVFNDKHLSWNW
                     RQAKRMVEISRELGFPMLAGSSAPVAWRVPAVDTPYGARQRHAVAISFSGLDIYGFHV
                     LEALQCMVERRTGGETGVRAVQCLENQDCWNFIEQNGWVKRLFDSAITRSNTRKPGDP
                     KELVKKPAVFVIDYNDGLKAAAFLLTGLVQDFTVAVDVKGSSQPVATLMNLQSGRPHH
                     HFGCLVKNIEILFETGKPPYPVERTMLTSGILDFALESRIQDHRKLDTPELAQVRYRS
                     PEGSHFCTKGWGPDGKRLDL"
     gene            9450..10394
                     /locus_tag="VFQ79_14780"
     CDS             9450..10394
                     /locus_tag="VFQ79_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140986.1"
                     /translation="MRFFAPLVLLIAAQVFALDAVGRMERLQIGGEWCFIRHPARNLH
                     AGEAIVILHGNGHTVESTTSTWEQQRSLRGLMDRLSAAGYLIAQSNHTATRSNGMWGN
                     PESCRSVVALMRHLENSGVRRFHAISNSAGNLVLLNLLLSGQARFESAAAIAGVVSLE
                     SMYRCPAGANRVTGIAEAFAFSPASRCPGNPGSDTSFRNATADYDPLRRMQSMSDGEI
                     RRLLGNTRWLGLYETRDPKVLPEENILAFARRLKTAGVAVELRSLDLATHGAGELFDS
                     FTPEILRVLSGSRAENSAQARIFIPTPIFDSAYWVSRR"
     gene            complement(10331..11785)
                     /locus_tag="VFQ79_14785"
     CDS             complement(10331..11785)
                     /locus_tag="VFQ79_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140987.1"
                     /translation="MSRRSFLASGAAAAITPLAAASQNAVRQAPIAVPLKIQPVLTYE
                     IPTKREATSYRPWGGLLTEQDAAAERERIGAELKALSQAGGFRFLPLVSVQTPEQAAR
                     IAREGHDVVLMYAASGGGKTLEALVTPDKWSLMFLRHRSGPVYLWYEIAHPRFLRKTV
                     DEFGQPGMTVKDVVVDSQTDLAGKLRGLYGLKNTLGKRMVAIGGPSGWGAGGRLAPDH
                     AKNDWKFDLRTVSYDELGERIQKARTDARRVKRCDAMADDYLAQKEVSLETSRDFVRK
                     AFLLTDVFRDLLDEAQTDALTVNQCMGTIMKVSETTACLPLSILNDEGYMAFCESDFV
                     VIPSGVLLHYISGKPVFLNDPTHPHHGVVTLAHCTAPRKMDGRRSEPVRVLTHFESDY
                     GAAPKVEMSKGQVVTNLIPDFGNRNWTGFEGEIIDNPFMPICRSQIDVTIKGDTDTLL
                     EEMKGFHWMTSYGSYLRETQYALSKIGVGMKILA"
     gene            complement(11942..13372)
                     /locus_tag="VFQ79_14790"
     CDS             complement(11942..13372)
                     /locus_tag="VFQ79_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0140988.1"
                     /translation="MSIPRRKFLLGLTAGAGALRTSTKAAASDRPNIILCMADDQGWG
                     DVGYRSHPVLKTPVLDEMASSGLRFERFYAAAPVCSPTRGSVLTGRHPNRYGCFSWGH
                     TIRPQEVTIAEALKGAGYTTGHFGKWHVGSVRAESPVSPSGSGFDEWLSSPNFFENDP
                     LLSRNGKVIQTRGEGSQVTVDAAIEFIRSAIARKQPFLAVVWFGSPHAPHEALESDRR
                     LYSDQPEKLQHFYGEITAMDRAIGNLRKELRRLAIADNTLLWYNSDNGAIPVGSTGGL
                     AGGKSTLDEGGIRVPAILEWPVRIRKPRISLTPCGTVDIYPTLIELAGAKVRNQVQPL
                     DGNSIVPLIDGRKQERNRPLGFWVYPEPGIPVRSRDLLEALAREQSGGAPMPNSPTSR
                     QDSARLYSENHLPGPAAWIDGDYKLHRRAPHGSDATYALFDLAKDPRETVDLSTRDPE
                     RVRRMRTELEAWQKSVIRSLKGEDYL"
     gene            13439..14398
                     /gene="cas6"
                     /locus_tag="VFQ79_14795"
     CDS             13439..14398
                     /gene="cas6"
                     /locus_tag="VFQ79_14795"
                     /inference="COORDINATES: protein motif:HMM:NF021551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HEU0140989.1"
                     /translation="MQDGAGLAPVFEFFPLRFHFVAQEAIRFPAGKPANILRGGFGLT
                     FRRLVCVPECPGAEACTLRSSCAYARAFEPHAGAKGPSGLADAPRPFVFRARHLDSVL
                     LNPGQPFHFDFHLFDVERPPFAQLIEVFAELGREGMGPGRGKANLVGADVLDSACRPR
                     VPLFVRGDAPRELAPMPVVLPLGPATNSPTRVTVDFVTPTELKTAGGLASRPDFPVLF
                     SRIRDRISALKALYSRRPLDIDFQAMAKRAESVAIVHSDLRHIRIERRSSRTGQAHPL
                     GGFTGTVEYEGEIAEFLPYLQAARWTGVGRQTVWGKGEIVVRA"
     gene            complement(14411..14881)
                     /locus_tag="VFQ79_14800"
     CDS             complement(14411..14881)
                     /locus_tag="VFQ79_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0140990.1"
                     /translation="MADDRTLRKQLVEVLRGGHAHATFDDTVDGFPVKQAGVRPGKMP
                     HSAWELLEHMRIAQNDILEFSRSADYISPKWPDGYWPASPAPSGEAEWRKSVQLFRED
                     LAAFEALLQDPRQDLNRPFPWGDGQTLLREALLIIDHNSYHLGQLMLVRRVLEA"
     gene            complement(14932..16254)
                     /locus_tag="VFQ79_14805"
     CDS             complement(14932..16254)
                     /locus_tag="VFQ79_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140991.1"
                     /translation="MSRWVFAVLASAAGLAPLYAQVEAGVIGGVPLTHFILDHSAGSR
                     VGSSTVTSAPRRYTLGPSIGFGLSGPLSLEASALYKRFGFDTFSIGGFPTAGVVRPIV
                     SINSSTTGNSWEFPVLAKLRLRFLPGVNAYLSTGPSIRRLSGIRERGVRTERILVPAV
                     STVTTSFETSSPETMNRRTSFGAVFGAGLDFRAGPVRLSPGIRLTRWDTERTSSTSAP
                     SRLARTQAEAVLRLAYTTADRLEAPPAKIPCCLEFGVLAGVPLLGVSDIRAEALPSSV
                     LDAPARRYAAGALLDWRFHSRLSLEGSFLLRRVGHVETNTIVQYTTSLTAHSWEVPLL
                     LKWRPARIGPATVVIGGGPALRRVSHIEWVTKSLTDSFRSDGSFLNRSALGVAASGGV
                     EFKTGAMRLHPELRYTRFERPLYDFYYIRTRQDSLALLIGVNWARTSR"
BASE COUNT         3353 a         4790 c         4898 g         3324 t
ORIGIN      
        1 tttgtacttc ttggtctcca cttcccggaa aaagtcgtgc acgccgtcgg atacaatctg
       61 cctctcctgt ttggtcagct ccgcgaaagg cacatcgaag cgtaccttcc cccgcgcctt
      121 gcggcgaaac tcgtcgtaat accaggcgta ttgcggcttg gtccagggat ctaccgcgcc
      181 gtcttttaac gaaacccaac ggtccggcac gacgagatcc atgtcgtaat cgatggtgtt
      241 gccgaacccc tggcagcgcg ggcaggcgcc gaatggattg ttgaagctga acaaccgcgg
      301 ttcggggtct tcgaatacaa tgccgcattg cttgcaggca aacgtctcgc tgaaacgaat
      361 ccgccggacc ggttcgtgca cggcctgttc gaaaataact tcaccgcact cgcgataaca
      421 gatctcgatg ctgtcaacga tccgctggtg aatgccgggc gcaatcacaa gacggtctac
      481 aagcacaaac aacggctggt cgaaatcgat ttccagcagc gattcgggcg atgaaaactc
      541 gtacgtcccc ggcggctgat agaggcggtt gaaccctttc ttccggaggt cgaacaggcg
      601 atctctcaac gcctgcgcat cgtgtgatac ctgcaccgga aacaacacgt accagcgcga
      661 accttcgggc tcggccaaca ggcgttgcgc cacctggtct accgtatcgc gctccacgcg
      721 ctgcccgcac tgcggacagt atgttcggcc ggcgcgcgca aacagcaacc gcaaaaaatc
      781 gtacagttcg gtcgctgtgc ctacggttga ccgcggattg cgcgtggtgt tcctctgccg
      841 gatcgctatg ggtggagcga taccctgaat gtcgtcaacc gccggttttt ccatgcgctc
      901 cagaaactgg cgtgcatacg ccgacagcga ttccacgtac cgccgctgtc cctcggcgta
      961 gatggtatcg aaaaccagcg acgactttcc tgagcctgaa actcccgtga caaccgtcag
     1021 cgcgttgtgc ggaatcgcca gcgagatgtt cttgaggtta tgtacgcgag cgccgcgaat
     1081 cagaatggag ttttgccgtg agctatcggg tcggggtcct atgttcttat tataggagac
     1141 tcgcctgatg gatcccgctg aacgcattcg gaggaagctg ctcgactggt accgccgcgc
     1201 gcgccggcct ctgccttggc gtgccaccag cgatccgtat cgcatctggg tgtcggaagt
     1261 gatgctacag caaacgcggg ttgtggcggt tattccgtat tacgagagat tcataaagcg
     1321 tttcccggat gtaaaatcgt tggccgaagc ccccgaacag gaactgctgg catgttggag
     1381 cggattagga tactactcgc gcgcccgcaa cttacagcga agcgcgcaaa tgatagtgga
     1441 aatgggagga tttccggaaa atttcaaaag aatccgctcc cttcccggca tcggtgatta
     1501 taccgccgcc gcgattgcaa gcatcgcttt tggccttcca catgccgcgt tggacgggaa
     1561 cgtcctgcgc gttctcagcc gcctgtcgaa cgacgcgggc gatatccggg cgcgtgagac
     1621 gggagaacgg atatcggcgc tcgcttcacg cttgctcgac aggaagaacc ccggcgattt
     1681 caaccaggcg atgatggaac tcggagcgac tttatgcagg ccgaagcggc ctcagtgctt
     1741 attgtgcccg cttgcatcgg agtgcaatgc gttggcggcg ggtaggcaaa atgagctgcc
     1801 cgtgaaactg cgcggcgcgc aaacgattca attggaatat actttgctgc tggtgcggag
     1861 aaacgggtgt ttcttgttaa ggcaacgcaa tacggcttcc agaaagcttg cgggtttttg
     1921 ggaagccccc tgcacggccg atctgccggc ggcgcgagtg ggccgcgaga tcggccgctt
     1981 caaacactcc atcatgcatc acgagtatcg tgtgaccgtc cgatttgcct cggttgtgaa
     2041 gtgccctccc ggctttcgct ggattgacgc cagtaccctt gaacagcttc cgttaacaac
     2101 cgcaacaagg aaagctctaa agctgttatg acttgtgaaa tctagcccgg tttcatcggt
     2161 atttccggat tttccggtta aactgatctg gaaaactggg ccatgaaaca acgaaaacga
     2221 ttactgtttg caaaaagcgt cgcgatctta agcacgattc cggcgttgat atatggattc
     2281 gctagtggtc cgctacccaa caaatcgggg gcgcccggtg gtggtctctg caccgagtgt
     2341 cacgtcggga gcgcggctgg taccagcatc gcggttacgg ctgcctcggg aactacgtac
     2401 actcccggtc aggcacaaca gattacagta cgtattacag aggcggccaa cgtttacgga
     2461 tttcaggcga ccgcgcgcac gtccggaaat cagcaggctg gcagctttac ctctggcgcg
     2521 ggcatgaagg tcatttccgg cgaaggcttc cagtttatcg agcacaatac gccatcgagc
     2581 aacagtacct tcaccttcca atggactccc cccgctaacg cttctggcga catcacgatc
     2641 tatgcggcgg gcaatgccgc gaatgggaac ggagaccata ccggtgacca cattcacagt
     2701 ggcaggctca cgctaaccgc cgccggttcg cagcaacctc gtcccacgat cggacagaac
     2761 ggtgtattga acggcgccag ctttcagccg cagatcgcag ccggtacgtg ggtgtccgtg
     2821 ttcggaacga actttgcttc cgccgaacgt ttccttctcg gctccgattt aatcaacggc
     2881 aacacgctgc cgaccgaact cgaaggcgtg cgcgtcgaga ttgacggaag agcgtcgccc
     2941 atcggctacc tgggccccag tcagatcaac gttcttgcgc cggatggcac atcctcggga
     3001 ccggtgcgcg tggaagtgag aacggcagcc ggcacgagtg aagcggccat agtgacatcc
     3061 caggccgttg ctcccgcctt cttcctgtac gatccggaag gccgcaagta catcgccgca
     3121 cagcatggag tcggcaacgc tcctttaggc aaggcgggcc ttgttgcagg ctcgacgccc
     3181 gcggctccgg gcgagaccat cattctgtac gctacgggcc tgggtcccac cgatcccgct
     3241 gtgccagtgg ggcgaattcc gtcgggtgcc gcgcgtatcc gcgagaatat cacggtgcgt
     3301 ttcggtgaaa ccgccgccac ggtggactat gccggcgtga gcacctgttg cgccggacta
     3361 taccagatca atgtggtcgt gccggcgacg gtccaaagcg gagatgtagc ggtaagggcg
     3421 caaatcggct cgctccagac ccaaccggac accttcatca caattcaagc tcagcagcct
     3481 caggctcaac ctcaacctca acctcagccg gagccggaga ctccgtacgg cgagacgtat
     3541 tactaaacaa acctatgacc gcttctactt tgagatggta agtgaatcct cgcgatcgcc
     3601 aagagtccgc tattgttgtt cggaaacagc ggatcccttc ccgccgcacc caacccggtt
     3661 ctcaaaagaa cgggagccca ggtagacggc ggattgaatt gcacggcatg ccaccagact
     3721 tatgcgcccg cgaactcaga caaccgcggg cgcgttgtcg tcgagagcgc ttactacaaa
     3781 cccggccaga agcagatcgt tcgagtacgt gtcgaacatc cggaagcgca gcgctggggc
     3841 tttcagttaa cggcgcggac cgccagcgac gaaacgaaaa cggccggctc cttcacctca
     3901 tccgccagcg tgcgcgtgga gtgcgacgat ggttcgcttc gcggaagcct tccaccctgc
     3961 gccgaaggca cgctccagtt cgccaatcat acgatggaat cgacccgcaa tgggaccccc
     4021 ggtggtgtct ggtgggatat cgaatggact ccccccgcca ccgacgtagg ggatgtagtt
     4081 ttctatgcgg ccgggaatgc cgcaaacggg aacggcacaa atgttggcga caggatatat
     4141 accacagcac ttcgcgtcgg tgggtgcagt acggccgtga agccttcgat tcgcggggtg
     4201 gtgaatgcgg ctacgtatca gcctggagtc gcactgaaca cgattgtcag catattcggc
     4261 gcgggattcg ccggcgccgg acagatgcgt gccgccggaa tcggcgattt cgatcacgga
     4321 aacttcccga aggaactcgc ctgcatggcg gtggagatca atgggcagcg cgcgccggta
     4381 gtgtatgtgc aaggggacca gattaacgcg caggttccga cgatgccggt atcgggccct
     4441 gtcgaaatgc gtgtgctcgc caatcccgga gcgcccaatc agatggcgag cgatccggcc
     4501 accattcaga tgcagaacca cgcgccggct ctgttcacct ttggaagttc gaacagcgtc
     4561 gccgctgttg tgcccgggac cgggcaacct atcgccaatc cggacgtgat ccagaatggc
     4621 agacccgcac ggcccggtga aatcattgag ctgtacggca ccggtttcgg tgtcaccgaa
     4681 ccggtctatc aggctggtga gcgtccgtcg gaaggggctc gcatccgcga tcctttcacc
     4741 gtcaccatcg gtggaaccac gctgcgggcg gaggatgtta tctatggtgg catcgctccc
     4801 acgtcgatca gcggtctgtt tcagttcaat gtccggcttc ccgagtcgat ggcggatggc
     4861 gatctcccgg tggtaatccg catcggaggc cttgagaccc agaacggcgt gataattccg
     4921 gtaaaaagat agtagaatcg gaactaggta caacacccta gtcctatgaa agcagcgatt
     4981 ccgctgctgg ccttgttcgc tctttcgcct atgcttcagg cggcagagag cgggcagcgc
     5041 gtggccatta cttattcctc ggcaagcgta gtcaatgcgg cgagtaatgt tgcggtattg
     5101 gctcccaata ccattgccag catctacggc gaaggactct cctggaaaga acgcagcatt
     5161 tccgaggacg atattcgcag tggcaacatg ccgacaatat tgtcccagac cggtgtgcgt
     5221 gtaatcgtca acaatattcc ggcatgcatt tattatgttt cgccgggaca gatcaacttt
     5281 ctggttccgg cgaacctgtt acccggtccg gcaaccgttc gagtgacatt ggacggtgcg
     5341 gtgggtccga aggctgaggt cgaactgctg cccgccgcac ccgccttctt tcaactggac
     5401 gccgagacca tcgtggcgac tcacgccgac gaggcagcag ttaccgcgga gaatccggcc
     5461 aggcccggcg aaattgtagt gctctacctg actgggctgg ggcgaacgaa gccatggttt
     5521 cgttcggggg aaatcgtaac acaggctgca tggatcgaag accggtccaa gctgacgttg
     5581 ctgattgacg gcgagccggt ggagcgttcc cggatcgaat atgccggtgt gacgcccgga
     5641 tcagcggggt tgtatcagat caacgtcaag cttccggata gcatccggga gaaccccgag
     5701 attcgagtat taatggacga gcaggccagc caggaaggac tgcgtttacc agtgaatccg
     5761 aatccagcgc ctccagagcc ggaatagcaa cgtaacccgg gtgccgcggg gcacgtttac
     5821 aatagttggg gagtatctat gcgatatctg ctggcggtgg tgatttccgg ttgttgcctt
     5881 gcatacgggc aggaagccgg ccaggagaag gaatcggcgc gttgggagga agcatctcag
     5941 gcgagcactg ccggcgaata cgtggtggaa ccgggcactc gcgttccgct caaccttatc
     6001 aacagcatca gtaccaagca tgcggccgaa ggcgatcgcg tctacctgga aaccgctttc
     6061 cccatcctga ccaggggccg tatcgttatt cctccgggaa gctatgtcgc gggcacggtc
     6121 acacaggtga agcgccccgg acgtctcaaa ggcagaggag agttgtacct gcggtttgat
     6181 tcactgacgc tgcccaacgg cgtcacccgg gacttccgcg cgcgcatcgg cggcctggat
     6241 ggacgcgcca gcgaagaact cgacaggagc gaaggcaaga tcaagagcga aggcaacaaa
     6301 ggtggcgatg ctcgcacggt gggtgaggct accgccgcgg gtgcgtcggt gggagtgatt
     6361 gccggaagca ctgcgggccg cgccggaatg ggcgccggca tcggagcggc agccggcgct
     6421 acggcgggac tcgttggtgt gctgctttca cgcggcccgg aggcggtatt ggccaaggga
     6481 acgacggtgg aaatggtcct cgaccgcccg atccacttca cggaagagga agtggcgttt
     6541 acgcaaccgc cgccccgccg gtcctcaagc gatggacctg gtcccgcgcc aagcaggaaa
     6601 agccgcaacc tcccgatgcc cgggcgatat ccgctcggct ggatgtttta aacacgacgc
     6661 cgataaccac tctctggcgg tcgtggctcg aaaacgaatg ctgagccgcg agtgcgcgga
     6721 gccggctcgc gatttcccgg gattgctcca tttgagagtt gacacttttc tcaggtaaca
     6781 tcaccaaata ggagcaactt ctcgaaatga tgcagaacag gcgtagtttt cttcagggtg
     6841 cggccgtagc cgccgtggct ggttcagcct cccggatact aggggctaat gaccgggtga
     6901 acgttgccgt ggtgggactc ggtgggcgtg ggcgcgctca cgtcacggag tgggcgaaag
     6961 ttccagggtc gcgaattgtc gcactctgcg atgtagacca ggctgcgctg gaacgcggcc
     7021 aggcccttgc cgaaaaggcg acgggcgaga aggcgaaggg ctacgtcgag atgcggaagc
     7081 tcttcgagga taaagatatt gacgcggtct cgatgccgct gcccaaccat tggcatgcgc
     7141 tcgcaaccat ctgggcgtgc caggcgggaa aagacgtata tatcgagaag ccggcctgcc
     7201 ataacgtctg ggaaggcaaa aaaatggtcg aggccgcccg gaagtacaac cgcatggtcc
     7261 aaatcggctc gcaaagccgc actgtgccga tgaagatccg tgccatgcag cttttgaagg
     7321 aaggcgtgat cggcaaggtc tatcttgcca aaggattgtg ctacaagcgc cggccctcca
     7381 tcggtcacaa acctgatgga cccgtgccgc caggtgtgaa ctgggatctc tttctcggcc
     7441 ctgcccctat gcgcccgttc aatgagaacc gcttcaagta caactggcac tggttctggg
     7501 atacagggaa cggagacatc ggcaaccagg gcgtgcatga gatggacatc gcccgctggg
     7561 gcctgggggt ggcatacccg aaatcggttg tctctttcgg tgggaagtat atctacgatg
     7621 acgaccagga gacgccgaac acgcaatacg caagcctcga ttacggcggg atgcagatcg
     7681 atttcgaagt gcgcggaatt tttacggatg gaacccaggc catgccgcgg cagggccgga
     7741 acacggtggg agatttattc tacggcgccg acggatacat gtcgatagac gacgatggat
     7801 tccaggtcta caagggcgag aaacatgaaa aggtcatgga tgaaaaagcc gagcgcggag
     7861 taagctcgac catggcgcac atggacaatt tcgccaaagc cgtgcggagc cgcaattaca
     7921 aagacctcac cgcggatgtg gaattgggcg ttatctccgc cgacttgtgc cacctggcga
     7981 acatcgctta ccgtgtggga cggaagctga cgttcaatgc gcaggcgagc cagtttgtga
     8041 acgatgcggc ggcgagcaaa ctgttgactc gcgactatcg caaaccttac gtagtaccgg
     8101 ataaagtgta aaatcctctg gccggacggc tacagatcca gacgttttcc gtccggcccc
     8161 caacccttcg tacagaagtg cgagccctcc ggactcctgt acctcacctg tgccaattcc
     8221 ggcgtgtcga gttttcggtg gtcttgtatt cgagactcca gcgcgaaatc aagaatgcca
     8281 cttgtcagca tggtgcgctc gacagggtag ggcggcttgc ccgtctcaaa caggatttca
     8341 atgttcttca ccaggcagcc gaaatgatgg tgcggccggc ccgattggag gttcatgagt
     8401 gtggccacag gctgcgatga tcccttcaca tccaccgcaa ccgtgaaatc ctgaaccagg
     8461 ccggttaaca ggaatgccgc tgccttcagt ccgtcgttgt agtcgatgac gaacaccgcc
     8521 ggcttcttga cgagttcctt cggatctcct ggcttgcgcg tgttgctgcg ggtgatcgcc
     8581 gaatcgaaaa gccgtttcac ccaaccattc tgttcgatga aattccagca gtcctgattt
     8641 tccaggcatt gcacggcgcg aacacccgtt tcgcctcccg tgcggcgttc caccatgcac
     8701 tgaagcgctt cgagtacgtg gaaaccgtag atgtcgagtc cgctgaagga gattgcgacc
     8761 gcatgacgct gccgcgctcc atagggagtg tctacggccg gcacgcgcca cgcgactggt
     8821 gcggaagagc cggcgagcat cggaaagccc agttcgcgcg aaatctccac catgcgtttc
     8881 gcctgacgcc agttccacga gaggtgttta tcgttgaaca cgggaactgc tctgccgtac
     8941 ttccggaaaa cgtcggtgat cttcagaaac atctcgaacc gcggatagag tttctgttcc
     9001 ttgtcgttca tcggataatc accgtgttcg ccaatcagta ggacgccgtc caccgcaagg
     9061 ttgctgcctc ccagcgtcaa ggcgtcatag acggtacgaa aaatcggaac accgtatttc
     9121 tttgcccgtt cgcggctcat gtcggtcttc ggcacctgct ccgtgaacat cgagacgatc
     9181 tttgagcgtg ggtagggcgg ctggtcgtcc tgattgtaac cctccaggta ctttccgacg
     9241 atcacgtcgg cgtgcgagtt aggccggtac tcggtgatga tcgcggcaat cttcttcgga
     9301 ggaccggact gcgaggcacc cgcgaacacg gttgagccgg agagagcgag aaatgtacgt
     9361 cgattcatga ggaaccagga atggagaata gcgtatcaga aaagtcccac tggtaacttt
     9421 gcccggttaa tatttcaaga ggaggagcag tgaggttctt cgcaccgttg gtactgttga
     9481 tagccgcgca ggtctttgcg ttggatgccg tcggccgcat ggagcgcctt cagattggcg
     9541 gcgagtggtg cttcatccgg cacccagcac gcaacctgca tgccggcgaa gcgattgtga
     9601 ttttgcatgg caacgggcat acggtggaat ctacaacctc tacctgggag cagcagcgtt
     9661 cgcttcgcgg gctaatggac cgcctgtcag ccgccgggta tctgattgct caaagcaacc
     9721 acaccgctac ccgctcgaac ggcatgtggg gcaacccgga aagttgccgg tcggtggtgg
     9781 ccttgatgcg gcacctcgag aatagcggcg tgcgccgctt ccacgcgatt tccaactcgg
     9841 ccggcaacct ggtattgctg aacctgcttc tatcgggtca ggcacggttc gaaagcgcag
     9901 ccgccatcgc aggagtggtg tcccttgagt ccatgtacag gtgccccgct ggagcaaacc
     9961 gggtgacggg aatcgcggag gcattcgcgt tcagccccgc gtcgaggtgc ccgggaaatc
    10021 ccggcagcga cacatccttc cggaatgcga cggcggatta tgaccccttg cgccgtatgc
    10081 agtccatgag cgatggagaa atcaggcggc ttctgggcaa cacccgctgg ctggggcttt
    10141 atgaaactcg cgatccgaag gttcttcctg aagagaacat tctcgccttt gcgcgccgct
    10201 tgaaaacagc gggcgttgca gtggaattac gatcgctcga ccttgctacg cacggtgccg
    10261 gcgagttgtt cgattccttc actccagaaa ttctgcgcgt tcttagcggc tcccgcgcgg
    10321 agaattcggc tcaggcgaga atcttcattc caaccccgat tttcgatagc gcgtactggg
    10381 tttcccgtag gtagctgccg taggaggtca tccagtggaa gcccttcatt tcttcgagta
    10441 aggtatcggt atctcccttg atcgtaacgt cgatttgaga ccggcagatc ggcatgaagg
    10501 gattgtcgat gatctcgcct tcaaagccgg tccagttgcg atttccaaaa tcgggaatca
    10561 ggttcgttac tacctgcccc ttgctcatct ctacctttgg cgcggcgccg taatcggatt
    10621 caaaatgggt gagcactcga acgggctccg agcgccggcc gtccatttta cgcggggccg
    10681 tgcagtgcgc caacgtcact actccatggt gcgggtgagt gggatcattc agaaaaacag
    10741 gcttgcccga tatgtagtgc agcagaacac cggatggaat cactacgaag tccgattcgc
    10801 agaatgccat atagccttca tcgttgagga tgctcagagg caggcaggcc gtcgtctcgg
    10861 agactttcat gatggttccc atacactggt tcaccgtcag ggcatccgtc tgggcttcat
    10921 ccaacaagtc gcggaaaaca tcggtcaaca ggaacgcttt ccggacgaag tctcgcgacg
    10981 tctcgagtga gacttctttc tgcgccagat aatcgtccgc cattgcatcg cagcgtttca
    11041 cccgtcgcgc gtcggtccgg gccttttgga tgcgctctcc cagttcgtcg tagctcaccg
    11101 ttcgcagatc gaacttccaa tcgttttttg catgatcggg cgccagcctt cctcccgcgc
    11161 cccagccact cggaccaccg atcgccacca tgcgcttgcc aagcgtgttc ttcaggccgt
    11221 agagcccacg aagtttaccg gccaggtccg tctggctgtc taccaccaca tccttgacgg
    11281 tcatccccgg ctggccgaat tcatccacgg tcttgcggag aaatcgcggg tgcgcgatct
    11341 cgtaccaaag gtaaaccggt cccgaacgat ggcgcaggaa catcaggctc cacttgtctg
    11401 gggtgacgag cgcctccagc gtttttcccc cgcctgaagc ggcgtacatc agtacgacat
    11461 cgtgcccctc ccgggcaatc cgggccgcct gttccggcgt ttggacggaa acaagcggca
    11521 ggaaccggaa gccgcccgct tgcgatagcg ctttcaattc cgcgccgatg cgttcacgct
    11581 ccgctgcggc gtcctgctcg gtgagcaacc cgccccaggg ccggtaactc gttgcctccc
    11641 gctttgtagg gatttcgtac gtcagaacgg gctgaatttt cagggggact gcgatgggcg
    11701 cctggcgcac cgcattctgg cttgctgccg ccagcggggt gatggccgcg gcggcacccg
    11761 aagcgagaaa acttctccgc gacattcccg ggtcgcagca ggcgcgcgac cggctactgc
    11821 aggaagaaca gtgaagcgaa gtaaacagat gatttgaagc cgccataaca aactccgtat
    11881 gtcggcctga ccatatcaga gtttcagatc tggtgtcaat gaatcgctta gtttgtccgg
    11941 atcaaagata gtcttcgccc ttgagactgc gtataacgga cttctgccat gcttcgagtt
    12001 cagtccgcat acgccgcacg cgctccggat cccgggtgga cagatcaact gtttcccgtg
    12061 ggtccttcgc cagatcgaag agggcatacg ttgcgtcgct gccatgaggc gctcgacgat
    12121 gcagtttgta atcgccgtcg atccacgcgg caggccccgg gagatggttt tcggaatata
    12181 gccgcgcgct atcttgccgg gaggtgggtg agttgggcat tggagcgccg ccggactgct
    12241 cgcgtgccag ggcctcgagc aggtccctgc tgcgaacggg aatgccgggt tcagggtaga
    12301 cccaaaagcc caacggcctg ttccgttcct gttttcgtcc gtcgatcagc ggcacgatgc
    12361 tgttgccatc cagcggttgc acctggttcc tgaccttggc gcccgctaac tcgatgagcg
    12421 ttggatagat gtccactgtg ccgcagggcg tgaggctgat gcgcggcttc cggatgcgta
    12481 ccggccattc gagaatcgcg ggcacgcgaa taccgccctc gtcgagcgta ctctttccac
    12541 cggcaagacc accggtggaa ccgacgggaa tggcgccgtt gtcgctgttg taccagagca
    12601 aagtgttatc tgctatcgcg agcctgcgaa gttcttttcg caggttgcca atggcccggt
    12661 ccatggcggt aatctcgccg tagaagtgct gcaatttctc gggctgatcc gagtagagcc
    12721 gccggtcgct ctcgagtgcc tcgtgcggtg cgtgaggcga tccgaaccac accactgcga
    12781 ggaaaggctg ctttcgggca atggccgaac ggataaactc gatcgcggcg tccactgtta
    12841 cttgcgatcc ttcgccccgg gtctgaatga cctttccgtt ccggcttaac agtggatcgt
    12901 tctcgaagaa gttcgggctg gacagccact cgtcaaatcc actgcccgat ggcgacacgg
    12961 ggctctccgc ccgtacactg ccgacgtgcc actttccgaa gtgtccggtt gtgtagcccg
    13021 cgccctttag cgcctcggca atcgtcacct cctgcggcct gatcgtgtga ccccaggaga
    13081 agcagccgta gcggtttgga tgacggcccg tcagtacgct gccgcgggta ggagaacaga
    13141 ccggagccgc cgcgtaaaag cgttcgaagc ggaggccgga cgaagccatt tcatcgagaa
    13201 cgggtgtttt caataccggg tgagaccggt agcctacgtc gccccacccc tgatcgtcgg
    13261 ccatgcatag gatgatgttc ggacggtcgc tcgccgcggc cttcgtcgag gtcctgagcg
    13321 cgcccgcgcc cgccgtaaga cctagtagaa atttacgcct tgggatggac atggcgttat
    13381 tctatcccgc cggctgttag cggtctgtta cgatgtgtca ggttcgacgg tctcagatat
    13441 gcaagacggc gcggggcttg cccccgtctt cgagtttttt ccgctccgct tccactttgt
    13501 agcgcaagag gcgatccggt ttccggccgg caagcctgcc aatattttgc gcggaggctt
    13561 cggcctcacg ttccggaggc tcgtctgcgt ccccgagtgt ccaggtgcgg aagcgtgtac
    13621 gctgcgctcc tcctgcgctt atgcgcgcgc tttcgaaccg cacgccggag caaagggacc
    13681 gagcgggtta gcagacgcgc cacgtccatt cgtcttccgc gctcggcatc tcgacagtgt
    13741 gttgcttaat cctggacagc cgtttcattt cgactttcat ctgttcgatg tcgagcggcc
    13801 tccttttgct caactgatcg aggtttttgc cgaactgggc cgcgagggaa tggggcccgg
    13861 gcgcgggaag gcgaatctcg tcggggccga tgttctcgac tcagcctgcc gcccgcgcgt
    13921 gccactgttc gtgcgcggcg atgcgccgcg cgagttggcg cccatgcccg tcgtgttgcc
    13981 actcggtccc gctacgaaca gtcccacgcg cgttacggta gactttgtca ctccgaccga
    14041 actcaagaca gccggaggtc tggcatcgcg ccccgatttc ccggtcctgt tcagccggat
    14101 acgcgatcgc atcagcgccc tgaaggccct ctacagccgg cggccgcttg acatcgactt
    14161 tcaggccatg gcgaagcgcg cagagagcgt cgccatcgtc cactccgatt tgcgccatat
    14221 ccggatcgaa cggcgcagca gcaggaccgg ccaggctcac ccgctgggcg gcttcaccgg
    14281 cacggttgaa tacgagggag agatagcaga atttctgcca tacctgcaag cggcaagatg
    14341 gacgggcgtg ggccgccaga ccgtttgggg taagggtgag atcgtggtgc gagcctgacg
    14401 ggtttgcagg ctacgcttcg aggacccttc tcaccagcat caattgcccc aggtgatacg
    14461 aattatggtc gatgatcagc agcgcttcgc gaagcagcgt ctgtccgtct ccccacggaa
    14521 aaggccggtt cagatcttgc ctgggatctt gtaacagggc ctcgaatgcg gccaggtctt
    14581 cgcggaatag ctgtactgat tttcgccatt ccgcctcgcc ggaaggcgct ggagaagccg
    14641 gccagtaacc atccggccat tttggagaaa tgtaatcggc agaacggctg aattccaaaa
    14701 tgtcgttttg cgcgatacgc atgtgttcca ataactccca ggcggagtgt ggcatcttgc
    14761 cgggccgtac tccggcttgc ttcaccggaa agccatctac agtgtcatcg aacgtagcgt
    14821 gcgcatgccc gccgcgcagc acctcgacca gttgctttcg cagcgtccta tcgtctgcca
    14881 tggtgtcctc ccggtgacag tgtgccacga attgcatgag gccggtgcgt cttacctcga
    14941 agtccgcgcc caatttacgc cgatcaggag cgctaacgaa tcctgccgcg tgcggatgta
    15001 atagaaatcg tagagtgggc gctcgaatcg ggtatagcga agctccgggt gaaggcgcat
    15061 ggcgcccgtc ttgaactcta ctcctccgga tgccgccacc cccagcgccg aacggttaag
    15121 gaacgatccg tcggaacgaa aagaatctgt cagggatttc gtcacccatt cgatatgact
    15181 cacccggcgt aatgccggac ctccgccaat aacaaccgtt gccggtccga ttcgagccgg
    15241 ccgccatttc agcaatagag ggacttccca agaatgcgcc gtcaaggaag tcgtgtattg
    15301 aacgatggta ttggtttcca cgtggcccac tctgcgtaga agaaaactgc cttcgagcga
    15361 taggcgtgag tgaaagcgcc aatcgagcag tgcgccggcg gcgtaccggc gtgcaggcgc
    15421 atccagcact gacgatggca gggcctcagc ccggatgtcg cttacgccca acagaggcac
    15481 acccgccaat acaccaaact cgagacagca gggaattttg gcaggcgggg cttccaggcg
    15541 gtctgccgtt gtataagcga gccgcagcac agcctcagcc tgtgtcctgg cgagccgcga
    15601 tggggctgat gtgctcgatg tgcgctcggt atcccatctc gtaagccgga taccgggcga
    15661 gaggcgcacg ggcccggcgc ggaaatcgag tcccgcgccg aataccgcac caaaagatgt
    15721 gcggcggttc atagtctccg gcgaagacgt ttcaaaactg gtcgttaccg tggagactgc
    15781 cggcactagt atgcgttccg tgcggactcc gcgttccctg attccgctga gacggcggat
    15841 ggatggcccc gtgctgaggt aagcattcac accgggaagg aaacgcagac gaagttttgc
    15901 aagaacgggg aactcccagg agtttcccgt cgtcgaggag ttgatactca caatcggacg
    15961 gacaacccct gcagtcggaa acccgcctat cgagaacgta tcgaatccga accgtttgta
    16021 gagggcactg gcttccaggc tcaacgggcc agacaagcca aaccctatgg aaggtcccaa
    16081 tgtataccgg cgcggcgcgg aagtaaccgt gctggatccg acgcggctgc ccgccgaatg
    16141 atcgagaatg aaatgcgtaa gcggcacacc tccgattaca ccggcttcta cttgtgcgta
    16201 taacggcgcc agtcctgcgg cgctggcgag aacggcaaaa acccaccttg acatttatcc
    16261 gaatggtagc tccttgcgct cccttaaggg gggaggagcg tttccgcagt cttttttcct
    16321 attgcattcc aatacggcaa gcagtagtat tggttagcaa tagga
//
LOCUS       DASTMK010000340         4233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_304783, whole genome shotgun sequence.
ACCESSION   DASTMK010000340 DASTMK010000000
VERSION     DASTMK010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304783"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFQ79_14810"
     CDS             <1..376
                     /locus_tag="VFQ79_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140992.1"
                     /translation="LAMETPGVEGQSFNLAGPIRPSAEEFMEMARKRARRNFRFYPQS
                     LLKMQTIEILKWAVKCAARKRDNAWPSFRDLKSRSLRAQLDSTAAERILGWSPTTSTD
                     TFLAEAIDSHLQPLLAGDLRCA"
     gene            380..1531
                     /locus_tag="VFQ79_14815"
     CDS             380..1531
                     /locus_tag="VFQ79_14815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0140993.1"
                     /translation="MRDPIHILHVFSTFGLGGPQLRVANIINSLGPAFRHTILPMDRN
                     SDAACRIAPGARVHFIMPPGRDSGRPYAMALHHTIRDIHPELLVGYNWGAMDAVLAAV
                     VGRLCPVIQHEHGFGIDEAHALKFRRVLARRILLKAVHCTIVPSKVLYDIAITRYRLA
                     AEKVRLIPNGVDTERFRPDRNPELRGQLGPGGVLFGYVGQLRPEKNLGLLIQAFALAN
                     IESAKLVLIGDGPCRPELEHQARGLGVLGKVIFTGNVQDPVPYLGALDVFVMSSVSEQ
                     MPMSLLEAMACGLPALCTDVGDIREMLGEAGSPVVVPSENPGAYADAMRLLACNVETR
                     AALGAGNRERCIARYSLEHMVRSYEDEYYAALGLPHAPMSMRRTLRESA"
     gene            1578..2963
                     /locus_tag="VFQ79_14820"
     CDS             1578..2963
                     /locus_tag="VFQ79_14820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEU0140994.1"
                     /translation="MSDKAIPSSISGTPPAHLISVIIPVYNSASYLKLCLEHLQQSDI
                     PYECIVVDDRSTDGSAEVARQFGAKVLSTDRRSGPARARNIGAREATGDILYFIDADV
                     CVEPGTLRRIRANFEEDPGIDALIGSYDNSPQAQDFLSQYKNLMHCYTHQRGKKQACT
                     FWSGCGAIRREVFLECSGFDESYGRPAIEDIELGYRLKSDKKKIVLDTELQVKHLKAW
                     SFASLVKTDILDRGIPWTELILRDKNLPNDLNLQLSQRVSVALVYLLIGVAAVGAIYA
                     GATFVLPLLALFFLFLIGYETESTWQQNPRAIIGTIVLVAVIVALAFFTGSYWLVPPV
                     LLAYLLLFLRHRYAYGSERRRRMTGIFCGVYLLFVILFVLTYLPNNPFVFAFFVIFSI
                     VVALNSQFYVFLAGRTGRLLALAAIPFHLLFHFYNGISFAAGFTRHTLQRVFKREPQP
                     ESKRMAASSNQ"
     gene            2960..3724
                     /locus_tag="VFQ79_14825"
     CDS             2960..3724
                     /locus_tag="VFQ79_14825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0140995.1"
                     /translation="MTGSGTAMILTYHSLDTSGSVISVAPEAFRSQMEFLMRSGIPVV
                     PLTSLRTTPGAVAITFDDGFENFYTDALPVLTEYNLPATVFVVTDYCGDSNGWPSQSL
                     HGIPRRKLMDWTQLREAAGYGITLGAHTATHPHLPELPPARAEEEIRRSKHDLEERTG
                     TPVETFAYPYGDTSRAVRDCAAGYFRFACGTALGMVNDHSDPIELPRIDMYYFRNSFW
                     FKSLWSLHGRAYLALRRTLRAARQAQLRLHPSTAQS"
     gene            3721..>4233
                     /locus_tag="VFQ79_14830"
     CDS             3721..>4233
                     /locus_tag="VFQ79_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0140996.1"
                     /translation="MNQRTPIVILGAGPAGLGAALQLARRGPFDVTAVERHTQIGGNA
                     GSFDAGGLQVDYGSHRLHPACSPAILSDIREMLGADLLDRPRHGRMRIRGRWVHFPLK
                     PLDLAAHLPLSFSLGVLRDAAAKLGRKGSGESFEQVLQHGLGRTICRDFYFPYAKKIW
                     GVDPSELDAEQ"
BASE COUNT          804 a         1280 c         1273 g          876 t
ORIGIN      
        1 gctggccatg gagacgccgg gcgtcgaagg gcaatcgttt aatctggccg gacccatccg
       61 tccttccgcc gaggaattca tggagatggc gcggaaacgg gcacggcgga atttccgttt
      121 ctatccgcaa agccttttga agatgcagac gatcgagatc ctgaagtggg ccgtaaaatg
      181 tgccgccagg aagcgcgata acgcgtggcc cagctttcgc gatttgaaga gccggtcgct
      241 gcgcgcacaa ctggattcga ccgccgcgga gcggatcctg gggtggtcgc ccacaacttc
      301 aaccgacacg tttctggccg aagcgatcga ttcccatctt cagccgcttc tcgcggggga
      361 cctccggtgc gcctgaacca tgcgtgatcc cattcacatc ctgcacgtgt tttccacctt
      421 cggcctgggc gggccgcagt tgcgggtcgc aaacatcatc aactcgctcg gaccggcgtt
      481 ccgccacacc attctgccca tggaccgcaa cagcgatgcc gcctgtagaa tcgcaccggg
      541 tgcgcgcgtt cacttcatca tgccgcccgg gcgcgattcc ggcagaccct atgcaatggc
      601 gctgcatcac acgatccggg atattcaccc cgagctgctg gtgggctaca actggggtgc
      661 gatggatgcg gtgttggccg ccgtggtggg gcggctatgc cctgtgatcc agcacgaaca
      721 tggattcgga atcgatgagg cccacgcttt gaagttccgg cgcgtactgg cgcgacgcat
      781 cctcctcaag gcggtacatt gcaccatcgt tccctcaaaa gtgttgtatg acatcgcgat
      841 tacgcggtac cgcctggccg cggagaaagt ccgcctgatc cccaacgggg tggacacgga
      901 gcggttccgg ccggaccgca atccggaatt gcgcggacag ttggggcccg ggggcgtact
      961 attcggctac gtcgggcaac tgcggccgga aaagaatctg gggctgctga tccaggcttt
     1021 cgcgctggcc aatatcgaga gcgcaaagct cgttctcatc ggcgatgggc cgtgccgtcc
     1081 tgagctggag catcaggcac gagggttagg cgtactggga aaggtgatct ttacaggcaa
     1141 cgtacaagac cccgtaccat atctgggagc cttggatgta ttcgtgatgt cgtcggtttc
     1201 cgagcagatg ccgatgtcgc tgcttgaagc aatggcctgt ggtttaccgg cgttatgtac
     1261 cgacgtaggc gacatccggg aaatgcttgg cgaggccggc tctccggtgg ttgttccatc
     1321 ggagaaccct ggcgcctatg cggatgccat gcggctgctg gcctgtaacg ttgagacgcg
     1381 cgccgccctg ggcgccggca atcgggagcg atgtattgcc cgctattccc tggagcacat
     1441 ggttcgcagc tatgaggacg agtattacgc cgcgcttgga ctaccgcatg cacctatgtc
     1501 catgcggcgt actctccgtg agagcgcgtg agcagtaaca accatccgaa tttcgccaga
     1561 tacagaggta aagctcaatg agtgacaaag cgattccttc atcgatatcc ggaacaccgc
     1621 ccgcacacct gatttcagta atcattcccg tctataactc ggcatcgtac ctgaagcttt
     1681 gcctcgagca tctgcaacaa tccgacattc cctacgagtg cattgtcgtg gacgaccgct
     1741 ctaccgacgg ctcagcggaa gtagcgcggc agttcggagc aaaggtgctg tcgacggaca
     1801 ggcgcagcgg gccggctcgc gccaggaaca tcggggctcg cgaggccact ggcgacatcc
     1861 tgtactttat cgatgccgac gtgtgcgtgg agccgggtac gctgcggcgg atccgcgcaa
     1921 actttgaaga agatcccggc atcgatgcgc tgatcggctc ctacgacaac tcgccgcagg
     1981 cccaggattt cctctcccaa tacaagaacc tgatgcattg ctacacgcac cagcggggca
     2041 agaagcaggc ctgcacattc tggagcggct gcggcgccat tcgcagggaa gtatttctcg
     2101 agtgcagcgg attcgacgaa tcctacggcc gtcccgctat cgaggatatc gagctgggat
     2161 accggttgaa gagtgacaag aagaagatcg ttctcgatac ggaactgcaa gtgaagcatc
     2221 tgaaggcctg gagcttcgcc agcctggtca agaccgatat cctggaccgc ggcattccgt
     2281 ggaccgagct gatcctgcgc gacaagaacc tccccaacga cctgaacctg cagttgagcc
     2341 aaagagtaag cgtcgcgctg gtgtatcttt tgatcggcgt tgcggcggtg ggagccatct
     2401 atgccggcgc cactttcgtc ctgccgctgc tggcgctttt cttcctgttc ctgatcggct
     2461 acgagaccga aagcacctgg cagcagaatc ccagagcgat catcggaact attgttctgg
     2521 ttgccgtcat tgtcgctctg gcatttttca ccggaagcta ctggctggtt ccaccggtac
     2581 tgctcgctta cctgctcttg ttcctgcgcc atcgctatgc ttacggcagc gagcgccggc
     2641 gccgcatgac cggcatcttc tgcggagttt acctgctgtt cgtcatcctg ttcgtgctga
     2701 cgtatctgcc caacaacccg ttcgtgttcg cgttcttcgt gatcttcagc atcgtggttg
     2761 ccctgaactc gcagttctac gtattcctgg cgggcagaac cggcaggctg ctggcgctgg
     2821 cggccatccc gtttcacctg ttgttccact tctacaacgg aatctcgttc gcggccggat
     2881 tcacacggca cactctgcaa cgcgtgttca aacgcgagcc gcagcccgaa agcaaacgga
     2941 tggcggcgtc ttcgaatcaa tgaccggctc cgggacggcg atgattctga cctaccactc
     3001 gctcgatacg agcggaagcg tgatctcggt tgcgccggaa gcgtttcgct ctcaaatgga
     3061 gtttctgatg cgaagcggaa ttcccgtggt gccgctcacg tcgctcagga caacaccggg
     3121 cgccgttgcc atcacgtttg atgacggttt tgaaaacttc tacacggatg cgctgcctgt
     3181 tctaacggag tacaatctgc cggcgaccgt atttgtagtg acggattact gcggagactc
     3241 gaacggctgg ccgtctcaat cgctccatgg gattccgcga aggaagctga tggactggac
     3301 acagttgcgg gaagcggcgg gttacggtat tacgctgggg gcgcacacgg cgacacatcc
     3361 gcatctgccg gagctgccgc cggcccgggc cgaagaggag atccggcgct ccaagcacga
     3421 tctcgaagag cgcaccggta cgcccgtgga aacgttcgcc tatccttacg gagataccag
     3481 ccgcgcggtt cgcgattgcg ccgccggata tttccgcttt gcctgcggca cggcgctcgg
     3541 catggtcaac gatcattcgg atcccatcga attgccgcgg atcgacatgt actacttccg
     3601 gaattccttc tggtttaaat ccctgtggag cctgcacggc cgggcttacc tggccctgcg
     3661 ccggacgctt cgagcggcga gacaagctca actccgtctc catccgtcta cagcacaatc
     3721 gtgaatcaac gtactcctat cgttattctc ggcgccgggc ccgctggctt gggagcggcg
     3781 ctccagttgg cccgccgcgg gccgtttgac gtaaccgcgg tggaacggca cacacaaatt
     3841 ggcggcaacg ccggaagctt cgacgccggc ggtttgcagg tggattatgg aagtcaccgg
     3901 ctgcatccgg cctgctcacc cgctatcctg tccgacatcc gcgagatgct gggggcggat
     3961 ctactggacc ggccgcggca cggacgcatg cggatccgtg gccggtgggt gcacttcccg
     4021 ctaaagccgc tcgacctggc ggcccatctg cctctgtcgt tctcgctcgg cgtattgcgt
     4081 gacgcggccg ccaaactggg ccgcaaaggc agcggcgaga gcttcgaaca ggtcttgcag
     4141 cacggtctcg gcaggaccat ctgccgcgat ttctattttc cttacgcgaa gaagatctgg
     4201 ggtgtagatc cgtcggagct cgatgccgaa cag
//
LOCUS       DASTMK010000341         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_18161, whole genome shotgun sequence.
ACCESSION   DASTMK010000341 DASTMK010000000
VERSION     DASTMK010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18161"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VFQ79_14835"
     CDS             <1..794
                     /locus_tag="VFQ79_14835"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0140997.1"
                     /translation="FLKYDWCSYGKIAPDKSLASLQAPYRQMGDILKGLPRDIVFNLC
                     QYGMGDVWKWGAEIGGHCWRTTGDLGLEKSTRLPGFYSIGFKNAANFEYAKPGSWNDP
                     DYILIGHVGNASRIQDPPKLTSLTADEQYSYMSMWALMAAPLVFSGHMGYLDDFTLNV
                     LCNAEVIEVDQDPLGKQARVVRRTEEEFVLAKPMADGSLALGLFNLSESERRIEASLT
                     ELHLEGSYRARDVWRQRDSGQVAGEYSATVRPHGVSFVRLFPGKN"
     gene            complement(860..3118)
                     /locus_tag="VFQ79_14840"
     CDS             complement(860..3118)
                     /locus_tag="VFQ79_14840"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU0140998.1"
                     /translation="MKRSRWFALLGVVIAALAACTVFRPADAPVEKLQITGLREPVEI
                     LRDRWGVPHIYAQTSDDLFFAQGYIAARDRLFQIDLWRRIGTGELAEVLGSGAVSRDR
                     LARAVRFRGDWAAEWESYAPDAKQIATAFANGINAYIRGLDGKRPLEFRLAGYDPGLW
                     EAEDCAARVAGLLMVRNLPREVQRSRDTAEFGLPALTRYLAPDPPVSLRVPAGLNLGD
                     ITPDILEVYRDVLSPVRFRAEEGSNNWAVDGTLTKTGKPLMASDPHRPLQIPSLRKTV
                     HLVGPGWNAIGAGEPALPGIALGHNEHIAWGFTIAGIDQADLYVEELNPANAGEYRYK
                     GAWRKFDLEEAKITVKGQTSPQVVELKYSIHGPVIHEDRVRNRAYALKWVGAEPGTAG
                     YLASLSMARARSWAEFLESAARFKVPSQNMVYADRDGNIGWQVVGLAPVRKGWHGLFP
                     VPGASGAYEWTGFLPPSELPRLFNPPNHFVATANHNMLPSGYRHPIGYEFAAPFRHQR
                     VVEMLESGGKFDIGDFERMQQDVVSIPARRFQGVLKKWQPDAGSRAARVHEMLLSWDA
                     SLDAESREAMIYEAWIAKLPEAVFGPRLGARVDLVTLLAELEKEPRSAALENALKAAL
                     AGLEKDFGADMDQWTWGRAHTLRFQHVLREERFNRGPMPRPGDGNTVNSTSGPGFRQA
                     NGASYRHIADLSDWDRSVMTNTPGESGDPDSPHYADLLEDWSKGKYHPMPFSRAAVEG
                     ATTERILLEPVK"
     gene            complement(3130..3513)
                     /locus_tag="VFQ79_14845"
     CDS             complement(3130..3513)
                     /locus_tag="VFQ79_14845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0140999.1"
                     /translation="MILGIEHTAIASSDPHSLADWYVSNLDFVVNYKSSSSKTVFVKA
                     PNGSMLEIIEANASPRSPQTLRDAGIRHLAIAVSDFDKAYARLKENGVEFLTQPETSK
                     GNRVVFFTDPEGNYLHLLQREKPLP"
     gene            3585..4973
                     /locus_tag="VFQ79_14850"
     CDS             3585..4973
                     /locus_tag="VFQ79_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141000.1"
                     /translation="MLRLPILFFLFIAVAPAKATVYVLLWFDTEDYIEPASDDAALRI
                     ANDLSAAGVRATFKVVGEKARVLEARGRRDIFRALAPHDIGYHTDWHSVPPAPAVYLR
                     TLGWLEGAAEFERRERQGIADVTRIFGIAPSCYGQPGSSWAPQALRALKRMGIGTYVD
                     EGSQIGVNEQPFWLDGMLNVFNMGQFSLRASLEKGARISDDIARFDAAVKKLGAQGGG
                     VISIYYHPTEFVTTEFWDGVNFPGGRMTEREQWKSPQRRSAEDSERCFNILKQYVEHA
                     RTRPEVQFLTARELRRIFDGLRPPALDRSIAARHLAEHITFLTTENGSYSAADLLQIL
                     LGLEPRDVEGPSERGQTNFEGAMIPRSAFERAKRDAISAIETLNRLPANLWLGSQQLS
                     LIDFAATLASDSGATAGVALRRGKPEMEQYVARNPTAPFRWPIHPEGFTAPELLELAR
                     LQAWTLKPARLR"
BASE COUNT         1055 a         1596 c         1526 g         1035 t
ORIGIN      
        1 attttctgaa gtacgactgg tgctcctatg gcaagatcgc gcccgacaag tccctggcga
       61 gcctgcaagc gccctaccgt cagatgggcg acatcctaaa aggactgccg cgcgatatcg
      121 tgttcaactt atgccagtac ggcatgggcg atgtgtggaa gtggggtgca gaaataggtg
      181 gccactgctg gcgcacgaca ggcgatctcg gccttgagaa atcgacgagg ctgcccggct
      241 tttacagtat cggattcaaa aacgccgcga acttcgaata tgcgaagccg ggaagctgga
      301 acgatcccga ctacattctc atcgggcacg tcggcaacgc atcccgcatt caagacccgc
      361 ctaagctcac ttcgctgacc gccgacgagc aatacagcta tatgtcgatg tgggctctta
      421 tggccgcgcc gctggtcttt tcgggccaca tggggtacct cgacgatttc accttgaacg
      481 tactgtgcaa cgccgaggtg atcgaggtgg atcaggaccc gctcggaaaa caggcgcggg
      541 tggttcgccg tacggaagag gagttcgttc tcgccaaacc gatggcggac ggctcgcttg
      601 ccctcgggct attcaatctc tccgaatccg aacggcggat cgaggcgagc ctgacggagc
      661 tgcatctcga aggctcctat cgcgcgcgcg acgtctggcg ccaaagggat tccggccagg
      721 ttgccggcga gtactcggcc accgtgcggc cccacggcgt ttcttttgtg agattgtttc
      781 cagggaagaa ctaaacacga cgccactaac cgctccctcg cggtcgcggc tcggtaaacg
      841 agggagcgga tgcgcaatct tacttcaccg gctccagcag aatgcgctcc gttgtcgcgc
      901 cttccactgc tgcgcggctg aagggcatcg ggtgatactt gcccttgctc caatcttcga
      961 gcagatcggc gtaatgcggg ctatccgggt cgccggattc gccaggcgtg ttcgtcatca
     1021 ccgaacggtc ccaatcgctc aaatccgcga tgtgccggta ggatgctccg ttcgcctgcc
     1081 ggaatccggg accgctggtc gagttcaccg tattgccgtc tccgggacgg ggcatgggtc
     1141 cgcgattgaa acgctcctcc cgcaagacgt gctgaaagcg caaggtgtga gcgcgccccc
     1201 aggtccactg gtccatatcc gcgccgaaat ctttttcgag cccggcgaga gccgctttca
     1261 aagcgttctc gagcgccgca gaacgcggct ccttttcgag ttccgccagc aacgtgacca
     1321 ggtccacccg cgcacctaat cgcggaccga acacggcttc cggcagtttt gcgatccatg
     1381 cctcgtagat catcgcctcc cgggattccg catcgagcga cgcatcccag gagagcagca
     1441 tttcgtgaac gcgggcagca cggctacccg cgtcaggctg ccattttttc agcacacctt
     1501 gaaaacgccg cgcgggtatc gaaaccacat cctgctgcat tcgctcgaaa tcgccgatgt
     1561 cgaacttccc gccggattcg agcatctcca ctacacgctg gtgccggaag ggcgccgcga
     1621 actcgtatcc gatcggatgc cggtagccgg aaggcagcat gttgtgattc gccgtcgcga
     1681 caaaatgatt gggcggattg aacagccgtg gcaattcgga aggaggcaga aaacctgtcc
     1741 attcatacgc gccgcttgca ccgggcacgg ggaacaggcc gtgccatcct ttccgcacgg
     1801 gcgcgagtcc gaccacctgc caaccgatgt tcccgtccct gtcggcatac accatgttct
     1861 gggacggcac cttgaaccgc gcggccgatt ccagaaactc cgcccaactc cgggctcgcg
     1921 ccatggaaag cgaggcaagg tatcccgccg tcccgggttc cgcgcccacc cacttgagcg
     1981 cataagcgcg gttccgcacg cgatcttcat gaatgaccgg tccgtgaatc gagtacttca
     2041 actccacaac ttgaggcgac gtctgccctt tcaccgtaat cttcgcctct tcgagatcga
     2101 acttgcgcca tgcgcctttg tagcggtact caccggcgtt ggccgggttc aattcttcga
     2161 catagaggtc ggcctggtcg atgccggcga tagtgaatcc ccaagcgatg tgctcgttgt
     2221 gtccaagtgc aataccgggc agcgcgggtt cgccggcacc gatcgcgttc catccgggcc
     2281 caaccaggtg cacggttttt cgcagtgacg ggatctgaag agggcggtgc gggtcgctcg
     2341 ccatcagcgg tttgcccgtc ttcgtaagtg tgccgtctac ggcccagttg ttgcttccct
     2401 cctccgcgcg aaaccgaacc ggactcagaa cgtcgcggta gacttccaaa atatccggag
     2461 tgatgtcgcc cagattcaga cctgccggga cgcgcagcga aacgggagga tccggcgcaa
     2521 ggtagcgcgt gagcgccggc agtccgaatt cagcggtatc gcgcgaccgc tgcacttcac
     2581 gcggcagatt acggaccatc agaagaccgg ccactcgcgc ggcacaatct tccgcttccc
     2641 acaaacccgg atcgtagccc gccagacgga attcgagcgg ccgctttcca tcgaggccgc
     2701 ggatgtacgc attgatgccg ttcgcgaacg cagttgcgat ctgtttcgca tccggcgcgt
     2761 agctttccca ctccgccgcc cagtcgccgc gaaagcgcac ggcgcgggcc agcctgtccc
     2821 gcgaaacggc cccggagcct aacacttcag ccagttcccc cgtcccgatg cggcgccaca
     2881 gatcgatttg gaacaggcgg tcccgcgcgg cgatataacc ttgcgcaaag aacaggtcgt
     2941 cgctcgtttg cgcgtagatg tgcggcacgc cccaacggtc gcgcagaatt tcaacgggct
     3001 cgcgaagtcc agtgatctgc agcttttcaa cgggcgcatc cgccggccgg aatacggtac
     3061 acgcggccag tgccgcgata acaactccta atagtgcgaa ccatcggctc cgtttcatag
     3121 tcagttagtt tatggaaggg gtttttcgcg ctgcaacaag tgcaagtaat taccctccgg
     3181 gtcggtaaag aagacgacac ggtttccttt gctcgtttcc ggttgcgtca aaaactcaac
     3241 cccgttttct ttgagacgcg cgtatgcctt atcgaaatcc gaaacggcta tcgcgaggtg
     3301 ccgtatgccg gcgtctcgaa gcgtctgcgg agatcgcggc gacgcgttgg cctcgatgat
     3361 ctccaacatg gagccattgg gagctttcac gaaaaccgtc ttgctgctgg aagatttgta
     3421 gttgacgaca aaatcgaggt tgctcacgta ccagtccgcg aggctatgcg gatcggacga
     3481 agcgatagcg gtatgttcaa tgccgagaat cataattgga aattctaacg caaacccacc
     3541 accggagcct gtgcaattcg agtgcggtat catgaggctc acccatgctt cgccttccca
     3601 ttctgttctt tttgtttatc gccgtggctc cggcaaaggc gacggtatat gtcttactct
     3661 ggttcgacac cgaggattac atcgaaccgg catccgatga tgcggcgctg cgaatcgcaa
     3721 acgatttatc cgccgcgggc gtccgtgcga cgttcaaagt ggttggcgaa aaagctcgcg
     3781 tgctcgaagc gcgcgggcgg cgcgacatct tccgtgcatt agcgccgcac gacatcgggt
     3841 atcacaccga ctggcacagc gtgccgcccg ctcccgcggt ttatctgcgt actctcggtt
     3901 ggctggaagg cgctgccgag ttcgaacggc gggaacggca gggcattgcc gatgtcacgc
     3961 gaatctttgg catcgcgccg agctgttatg ggcagccggg cagttcctgg gcgccccagg
     4021 cgctccgtgc cttgaagaga atgggcatcg gcacgtatgt cgatgaaggc tcgcagatcg
     4081 gagtgaatga gcagcccttc tggttggacg gcatgctgaa cgtcttcaac atggggcaat
     4141 tcagcctgcg cgcttcgctc gagaagggcg cccgcatcag cgacgatatc gcgcgtttcg
     4201 atgcagcggt gaagaagctc ggcgcgcaag gcggcggcgt catcagcatc tattaccacc
     4261 ccaccgaatt cgtaaccacc gagttctggg atggagtgaa tttcccgggc ggccgcatga
     4321 cggaacggga acagtggaag agtccgcaac gccggtcggc ggaggactcc gagcgctgct
     4381 tcaacatcct gaagcaatat gttgaacatg ccaggacgcg gcccgaggtc caatttctca
     4441 cagcgcgtga attgcggcgc atttttgacg gactgcggcc tccagcgctc gatcgttcca
     4501 tcgcggctcg ccatctggcg gagcacatta cttttctcac caccgaaaat ggatcttatt
     4561 ccgccgcgga tctgctgcaa attttgctgg gattggagcc ccgcgatgtc gagggtccca
     4621 gcgaacgggg acaaacgaat tttgaaggcg cgatgattcc ccgctccgca ttcgaacgag
     4681 ccaagaggga cgctatctcc gccattgaaa cgctgaaccg ccttccggcc aacctgtggt
     4741 tagggtccca acaactttct ctgatcgact tcgcggccac gctggcatcg gacagcggcg
     4801 caacggcggg tgtcgcgctc cggcgcggaa agccggagat ggagcagtat gtagcccgga
     4861 atcctaccgc gccattccgc tggccgattc atccggaggg cttcaccgcg ccggaactgc
     4921 tggaactggc ccgcctgcag gcgtggacac taaagccggc caggctcagg tagaataccg
     4981 caccatttac cacgcttttt actggatttt tacgaaatca ggcgctgaat cacctctgaa
     5041 actcgcgtaa tcgaacagtg tgccgttccg gcgcatcagt aaacaaaaca tcagagaggt
     5101 aagaagtatg gcaaacccta ttgttgcccc cattcagttt ggcgacgcga ggtcggcggc
     5161 tgacaatttc cgcagaacaa ggaaaggcgt cgaggacatc gaaggcgtaa tc
//
LOCUS       DASTMK010000342         5586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_592813, whole genome shotgun sequence.
ACCESSION   DASTMK010000342 DASTMK010000000
VERSION     DASTMK010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592813"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFQ79_14855"
     CDS             <1..400
                     /locus_tag="VFQ79_14855"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0141001.1"
                     /translation="IQGEANAIQPNKRPLSSMTPTILLRDGSLYMVVGSPGGSRIITT
                     VLEVILNVVDFGMNIQDAVNWPRFHHQWQPDKLFVEWRHSPDTKALLQSRGHQVEDIR
                     SMGEVSAILVDRGWLEGAADPRSEGNAEGY"
     gene            406..1248
                     /locus_tag="VFQ79_14860"
     CDS             406..1248
                     /locus_tag="VFQ79_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0141002.1"
                     /translation="MKHVLSTHLIVNHRLNTVWLDRIWKAGFPAVEIFCARQHLDYRD
                     NGQINELANWFADAEIQFHSLHAPMYTDEVWGRSGPHSVISITETAKARRNASVDEIK
                     RALEIAEKIPFRYLIQHLGVGGEEYDERKLDAAFNALDEIKIFAKQRGVEVLLENIPN
                     GFSSASRLLNFLELTHLDLNFCFDVGHAHIAGGVETEYKSMQHRIRSTHIHDNDGTAD
                     IHLFPLHENGGTVDWKKTMTLLRSRPEQYPLLLELREVEGMPSPLNEAKSVIERLAAL
                     ETVE"
     gene            1245..2558
                     /gene="asnS"
                     /locus_tag="VFQ79_14865"
     CDS             1245..2558
                     /gene="asnS"
                     /locus_tag="VFQ79_14865"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU0141003.1"
                     /translation="MSSAPRITIADAAAHSGETVEIAGWLYNLRRSGKIVFPILRDGT
                     GLMQCVAVRSALPEETFEALRNLTQESSLIVTGKIRAEQRAPGGYELDVEQAEIVQRV
                     DEAEPYPITPKDHGIEFLMDHRHLWLRSRRQHAILRVRHEVIKAIRDYFDSHGFTLVD
                     TPIFTPAACEGTTTLFEVDYFEDEKVYLTQSGQLYNEAGAMSLGKVYCFGPTFRAEKS
                     KTRRHLTEFWMVEPEMAYATLEDVKQVAEELVVFVVSRVLENRRAELAVLERDVSKLE
                     AVQRPFPRMTYDEAVKILQEKGSEIQWGADFGGTDETLLAEGSDRPIMVDRFPAQIKA
                     FYFEPDPERPDVVLGVDVLAPEGYGEIIGGGQRIHNLDLLLKRLDEHHLPREAFNWYL
                     DLRKYGSVPHGGFGMGVERCVAWICGLEHVRETIAFPRMLYRTTP"
     gene            2562..3476
                     /gene="rocF"
                     /locus_tag="VFQ79_14870"
     CDS             2562..3476
                     /gene="rocF"
                     /locus_tag="VFQ79_14870"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEU0141004.1"
                     /translation="MRQSTIAIIGAPLDLGAGRRGVDMGPSALRVANLNSRLRGLGYE
                     VEDRGNIAVDQPESLPEGPAHARYLPQIARTCKKLADAVRSALAQNKVPLVLGGDHSV
                     AIGTLSGVAKHFRREKRSAGLVWVDAHADMNTPKTSPSGNVHGMPLACIIGKGPRELT
                     HLYGFAPKIDPKNVALVGVRDVDSLERPVVRNSGVRVFTMRDIDERGLRTVMREAIEI
                     ASAGTVGFHVSLDMDVVDPQEAPGVGTPVRGGITYREAHLIMEIISDSGCLISMEVVE
                     VNPVIDVGNRTAALGVELVMSAMGKRIL"
     gene            3473..4441
                     /locus_tag="VFQ79_14875"
     CDS             3473..4441
                     /locus_tag="VFQ79_14875"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEU0141005.1"
                     /translation="MKLRVAVIYGGRSGEHEVSLRSAEAIMSAMDPEKYKVLQYFIDK
                     DGKWRPRPIVAEPNGNPEIDVVFPALHGTFGEDGTVQGLLELADLAYVGPGVLASAAS
                     MDKEMAKRIFRERGLPVVEYVTAARGETAPGLPFDLPVFVKPANLGSSVGISRASTPA
                     ELAGALELAGQYDRKILIERAISGQELECSVLGNDEPIASIPCEILPSRAFYDYDDKY
                     LLNTAEFRIPAEIGESQIEEVRRLAVEAYKAIGCEGMARVDFLLESSSGKLYINEINT
                     IPGFTSISMYPKMWEHTGIPFAKLVDRLISLALERHEARKSIRYTR"
     gene            4467..>5586
                     /locus_tag="VFQ79_14880"
     CDS             4467..>5586
                     /locus_tag="VFQ79_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU0141006.1"
                     /translation="MFSFFPVLLCLAAAVESGDLPSQVKKFVDVLALLEQEAAEPVSP
                     YRAVYEGAIPGMLRTLDPHSVFFDPGQFDQLKELQSSTRKGFGSVVSVLPGRVIVLQT
                     LPGTPSAKAGMMAGDQIVAVNGIRLDYLDIEQLIGLLSESRRRQVSLDVRRAGNARIQ
                     HLVLTPEEMQSPSVERAFLLEPGIGYVRVTSFDTDTASQIRQAIERLGGSSLTGIVLD
                     LRDNPGGVLPAALETASLFLEPGQNLLSVKGRSVTGEQMKVPDAAKPYRFRVSVLLNG
                     RSASASEIVAGALQDYRRAAIVGEPSFGKGLVESVYPLSGGAGLALTTAFYYTPNGRS
                     IQRPLPGTQLDAATRAGDGGIKPDFPVWPESHTRLGAVL"
BASE COUNT         1173 a         1527 c         1772 g         1114 t
ORIGIN      
        1 cattcaggga gaggcgaatg ccattcagcc gaacaaacgc cccttgtcgt ccatgacgcc
       61 aaccattctg ctgcgcgacg gcagcctgta catggttgtc ggctcgccgg gcggttcgcg
      121 gatcatcacg accgtgctgg aagtgatttt gaacgtcgtt gatttcggta tgaacattca
      181 ggatgccgtg aactggccgc gcttccatca ccagtggcaa ccggacaagc tgtttgtgga
      241 gtggcgccat tcccccgata cgaaggcgct gctgcaatcg cgggggcacc aggtggagga
      301 tatccgctcc atgggtgagg tgtccgcgat attagtggac cgcggctggc tcgaaggcgc
      361 ggccgatccc cggtccgaag ggaacgccga aggctattag atcaaatgaa gcatgttctt
      421 tcgactcacc tgatcgtgaa ccaccgtttg aatacggttt ggctcgatcg catctggaag
      481 gccggctttc cggcggtaga aatcttttgt gcccggcagc atctggacta ccgcgacaac
      541 ggacagatca atgagcttgc caactggttc gcggatgccg aaatacaatt tcattcgctg
      601 catgcgccga tgtatacgga cgaggtgtgg ggacggtcgg gtccgcattc ggtaatcagt
      661 atcacggaaa cagcgaaggc gcgccgcaat gcttccgtgg atgaaatcaa gcgcgcgctc
      721 gagatcgccg aaaaaatccc gttccgctac ctgatccagc atttaggcgt ggggggcgaa
      781 gagtacgacg agaggaaact cgacgccgca ttcaacgcac tcgacgagat caagattttc
      841 gcgaagcagc gcggcgtgga ggtgcttctc gagaatatcc ccaatgggtt ctcgtcggcg
      901 agccgcctgc tgaattttct ggagctcacc catctggacc tgaacttctg cttcgacgtt
      961 gggcatgcgc atatcgccgg cggcgtcgaa acggaatata agtccatgca gcatcgcatt
     1021 cgttccacgc acatacacga caacgacggc accgcggaca tccacctgtt tcctttgcac
     1081 gagaatggcg gaacggtgga ttggaagaaa acgatgacgc tgttgcgctc gcggccggag
     1141 cagtatccgc tgttgcttga attgcgggag gtggaaggaa tgccgtcccc gttgaacgaa
     1201 gccaagtccg tcatcgagag gcttgcggca ttggagactg tcgaatgagc tccgctccca
     1261 ggatcacgat cgccgatgcg gccgcccact ccggcgaaac cgtcgagata gccggatggc
     1321 tctataacct gcggcgttcc ggcaaaattg tgttcccgat ccttcgcgac ggcacaggcc
     1381 tcatgcaatg cgtagccgtg aggagcgcat tgccggagga aacattcgag gcgttgcgga
     1441 accttacgca ggaatcgtcg ctgatcgtta ccggaaagat ccgggccgaa cagcgcgcac
     1501 cgggcggcta cgagctggat gtggaacagg ccgaaatcgt gcaacgtgtg gacgaagccg
     1561 agccgtatcc gatcacaccg aaagatcacg gtatcgagtt cctgatggac caccgtcact
     1621 tgtggctgcg cagcaggcgg caacacgcca tcctgcgcgt acgccacgag gtgatcaagg
     1681 ccatccgcga ttactttgac tcgcatggct tcactctggt ggatacgccg attttcacgc
     1741 cggcggcctg tgaaggcacc accacgctgt tcgaagtgga ttacttcgaa gacgagaaag
     1801 tttatctgac gcaatccggg cagctctata acgaggcagg agcgatgtcg ctcggcaagg
     1861 tgtactgctt cggcccgacg ttccgcgcgg agaaatcgaa gacgcggcgc cacctgacgg
     1921 agttctggat ggtagagccg gaaatggctt atgccacgct cgaagacgtg aagcaggtgg
     1981 ccgaagagct ggtggtgttt gtcgtttcgc gcgttctgga gaaccgccgc gcggaactgg
     2041 cagtgttgga gcgcgatgtt tcgaagcttg aagccgtcca acgtcccttc ccgcgaatga
     2101 cctatgacga agcggtgaag atccttcagg agaaaggcag cgagatccag tggggagcgg
     2161 actttggcgg taccgacgaa acgctgctgg ccgaaggatc agaccggccg atcatggtag
     2221 accgctttcc cgcacagatc aaggctttct atttcgagcc ggacccggaa cgtccggacg
     2281 tggtgctcgg cgtggacgtg ctggcgccgg aaggatacgg cgaaatcatc ggcggcgggc
     2341 agcgcattca caatctggac ctgctgttga agcgtctcga cgagcatcac cttccgagag
     2401 aagcgttcaa ctggtatctc gacctgcgta agtatggatc ggtgccgcat ggcggcttcg
     2461 ggatgggtgt ggagcggtgc gtggcctgga tctgcgggct cgagcacgtt cgcgaaacga
     2521 tcgcctttcc gcgaatgctg taccggacaa caccctgacc catgcggcaa tcgaccatcg
     2581 cgatcatcgg cgcaccgctc gacctgggcg ccggccggcg cggagtggat atggggccgt
     2641 cggcgctgag ggtcgcgaat ctgaattccc ggctcagggg cctgggatac gaagtggaag
     2701 atcgcgggaa tatcgctgtc gatcagccgg agagcctgcc ggaaggaccc gcacacgcaa
     2761 ggtatttacc ccagattgcc cgcacgtgca aaaagctcgc cgacgcggtg cggtctgcgc
     2821 tggcgcaaaa caaagtgccg ctggtattgg gcggcgatca ttccgtggca atagggacgc
     2881 tttccggcgt ggcaaagcac ttccggcgcg agaagcgatc tgccgggctt gtctgggtgg
     2941 acgcacacgc cgacatgaat acaccgaaga ccagcccgag cgggaatgtg cacggaatgc
     3001 cgcttgcctg catcatcggg aaaggcccgc gcgagctcac gcacctttac ggctttgctc
     3061 ccaagatcga tccgaagaac gtcgccctgg tgggcgttcg ggatgtggac agcctggaaa
     3121 gaccggtcgt acggaattcg ggcgtgcgag ttttcacgat gcgcgatatc gacgaaaggg
     3181 ggctgcgcac ggtcatgcgg gaggcgatcg aaatcgcatc ggcgggcacg gtgggcttcc
     3241 acgtttcgtt ggatatggat gtggtggacc cgcaggaggc tcccggagtc gggacaccgg
     3301 tacgcggtgg aattacatac cgcgaagccc acctcattat ggaaatcatc agtgactcgg
     3361 gatgtttaat ctcgatggaa gtcgtggaag taaatccggt gatagatgta ggaaaccgga
     3421 cggcggcgct gggagtcgaa ctcgttatgt ctgcaatggg aaagaggatt ctttgaaatt
     3481 acgcgtggct gtaatttacg gcggccgcag cggtgagcac gaagtttcct tgcgctcggc
     3541 cgaagcgatt atgtccgcga tggacccgga aaagtacaaa gtactgcaat atttcatcga
     3601 caaagacgga aaatggcggc ctcgcccgat cgtcgcggag ccaaatggca atccggaaat
     3661 tgacgtcgta tttccggcgt tgcacggcac tttcggcgag gacggaaccg ttcaaggcct
     3721 gctcgaactc gcggacctgg cttacgtcgg ccccggtgtg ctggcctccg ccgcctcgat
     3781 ggataaggaa atggcgaaac ggattttccg ggagcgtggt ctgccggtgg tggagtacgt
     3841 gacggccgcc cgtggcgaaa cggctcccgg gcttcctttc gatcttccgg tgtttgtgaa
     3901 accggcgaac ctgggatcgt cggtgggaat ctccagagct tcgactccgg ctgaactcgc
     3961 cggtgcactg gagctggccg gacagtacga tcgcaagatc ctcatcgaac gcgccatctc
     4021 cggccaggaa cttgaatgct ccgtattggg caacgatgaa ccgattgcat cgatcccctg
     4081 tgagattctg ccgtcacggg ctttttacga ctacgacgac aagtatcttc tcaacaccgc
     4141 ggagtttcgt ataccggcgg aaatcggaga atctcagatc gaagaagttc ggcggctggc
     4201 tgtcgaagcc tacaaagcca tcggatgtga aggcatggcg agagtggatt ttctgctcga
     4261 atcctccagc gggaaattgt acattaacga gatcaacacc attccaggat tcacgtctat
     4321 cagcatgtat ccgaagatgt gggaacatac ggggattccg ttcgccaaac tcgtcgaccg
     4381 gctgattagc ctggctctgg agcggcacga ggccaggaaa agtatccgct acaccagata
     4441 accgcacgat agactaaaag gagcggatgt tttctttctt tcccgtcctt ctgtgtctgg
     4501 cagccgccgt agagtctggc gatctcccgt cacaagtcaa aaagtttgtg gatgtactgg
     4561 cattgctgga gcaggaagcc gccgaaccgg tttctcctta ccgggccgtg tatgagggcg
     4621 ctattccggg gatgttgcga acgctcgatc ctcattcggt gttctttgat ccgggacagt
     4681 tcgaccagct caaggagttg cagagttcga cgcgcaaggg ctttggaagc gtggtatcgg
     4741 tgttgccggg gcgcgtgatc gtccttcaga cgctgccggg tacgccctcg gcgaaagcgg
     4801 gaatgatggc cggggaccag attgtcgccg tcaacggcat ccgcctggat tatctggaca
     4861 tagagcaact gatcggactc ttgagcgaat cccggcggag acaggtgtcg ttggatgtgc
     4921 gcagggccgg taatgcgcgc attcagcact tggtgctgac ccccgaagag atgcagtcgc
     4981 cgagcgtcga acgggcgttt cttctggagc ctggcatcgg ctatgttcgc gtcaccagct
     5041 tcgacacgga cacggcatcg cagatccggc aggccatcga gcgcctgggt ggatcatcgc
     5101 tgacgggaat tgtgctggat cttcgcgaca accccggcgg cgtgttgcca gcggctcttg
     5161 aaaccgcatc gctatttctg gagcccggac agaatctgtt gagcgtgaaa ggccgctccg
     5221 tcacaggcga acagatgaag gtgcccgatg ccgcaaaacc ttaccgcttc cgtgtatcgg
     5281 tgcttctgaa tggcagaagt gcgagcgcct ccgaaatcgt tgccggcgcg ttgcaggatt
     5341 accggcgggc ggccatcgtg ggagagccaa gctttggcaa agggctggtg gagtcggtgt
     5401 atcccttgtc cggcggcgca ggtctggcgt tgacgacggc gttttactat acgccaaacg
     5461 gtcgatcgat ccaaaggccg ctacccggta cgcagttgga tgcggctacc cgggcgggag
     5521 atggaggaat caagccggac ttccctgtct ggcccgaaag ccatacgcgg ttgggcgccg
     5581 tactgg
//
LOCUS       DASTMK010000343        17142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_207995, whole genome shotgun sequence.
ACCESSION   DASTMK010000343 DASTMK010000000
VERSION     DASTMK010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17142
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207995"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFQ79_14885"
     CDS             <1..853
                     /locus_tag="VFQ79_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141007.1"
                     /translation="YRKGEGFAGFLDGLPHILAGDSVRAVIAAIQDAREHGKPVIWAL
                     GGHVIKCGLAPVLIELMSRRYATAFAMNGAASIHDFEIALAGHTSEEVEAVLPDGTFG
                     SAEETGREMNRALSAADGIGAGEALGRALDELADSQFADKSLLLQAWRRSVPVTVHIA
                     IGTDTPHTHPAVDPDALGRSTHHDFRLFCSLVSALNEGGVYINCGSAVIMPEVFLKAV
                     SAVRNLGILLAGFTTVNLDFLQHYRPRVNVVERPHITAGGHGYSLTGHHELMLPLIAA
                     ALIEHDA"
     gene            850..1626
                     /locus_tag="VFQ79_14890"
     CDS             850..1626
                     /locus_tag="VFQ79_14890"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU0141008.1"
                     /translation="MSDPAGGMNGSPFRPALVPFVEETNRPYPFWGYRDIALFLSMAL
                     PSFILALLLMKVFLSVAPWYPRTKATELLPAQFLAYGLWFLSLYLLLHLKYGRPFWPS
                     LAWRVVPGQLRKSALLGLALAFGVAITGGLLKTPQIEMPFEDFLKDPLSIALVAVFAV
                     TLGPLFEELAFRGFFQPLLVRSAGAILGILIAALPFSLLHGPQYAWSWRHILLITLAG
                     CAFGWHRHRTGSTAAAAIMHSAYNLMFVTAYILQGRYATP"
     gene            1626..2675
                     /gene="mtnA"
                     /locus_tag="VFQ79_14895"
     CDS             1626..2675
                     /gene="mtnA"
                     /locus_tag="VFQ79_14895"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEU0141009.1"
                     /translation="MVETIRWTDDGVVMVDQTRLPREEVFVTCRTYREVADAIRRMVI
                     RGAPAIGVAAAMGVALGVLRADGDDLDAQMDEICGELAGTRPTAVNLFWAIRRMKDVY
                     GSLRGRPLEEIRRRLVEEAQQVRREDIAINEAIGRNGADLVPDGKTVLTHCNAGALAT
                     AGYGTALGVIRAAVAAGKSIDVFADETRPFLQGARLTAWELQQDGIPTTLITDNMAGH
                     FLKSGRIGCVVVGADRIAANGDVANKVGTYSVAVLAKENGIPFYVAAPVSTLDLSLAS
                     GDEIPIEQRDAAEVTTVFGTPVAPEGIPVENPAFDITPHRYVTAIITERGVARPPFGE
                     SLARISGSDTTGGRF"
     gene            2692..3213
                     /locus_tag="VFQ79_14900"
     CDS             2692..3213
                     /locus_tag="VFQ79_14900"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0141010.1"
                     /translation="MRLFVALLGCALALNGAGKLSGRRAPGFSLPDMQMRQHDLADYR
                     GKVVLIDIMRTNCPECQKLTPKLETVKAKYPGRVIVLSVVTLPDNQTTVAKYVAAYKV
                     TGPVLFDCGQMAASFVKATPQNPSMSLPHLFFVDTQGVIRNDFSDHEVSGLTVEALSA
                     EVERLLVPASKSR"
     gene            3275..4585
                     /locus_tag="VFQ79_14905"
     CDS             3275..4585
                     /locus_tag="VFQ79_14905"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEU0141011.1"
                     /translation="MPKDTLTITDNRTGKTYEIPIEHDTIRAMDLRQIKVSDGDFGLM
                     TYDPAYLNTAACKSTITYIDGDAGILRYRGYPIEQLAEHSTYLETAYLLYRGELPTSQ
                     QLDRWVTGVTHHTIPHEKIKYFMEGFRYDAHPMGIFVGTVAALSTFFPESKQVFDPEV
                     RRITFYRGVAKTPTIAAFAHRYSIGMPYAYPDNELSYTGNFLSMMFKGTELRYKPNPV
                     LERALDVLFILHADHEQNCSTSAMRVVGSAQTDPFTAIAAAAAALYGPLHGGANEAVL
                     RMLAEIGSKDKIPAYIKRVKAGEVKLMGFGHRVYKNYDPRARIIKQTADEVFEVTGKN
                     PLLDIALELERIALEDDYFVGRKLYPNVDFYSGLIYQAMGFPTDMFPVLFAIPRVSGW
                     LSQWEEMILDPEQKIARPRQVYLGEGERDYVPMEEREAVTATRS"
     gene            4636..5436
                     /locus_tag="VFQ79_14910"
     CDS             4636..5436
                     /locus_tag="VFQ79_14910"
                     /inference="COORDINATES: protein motif:HMM:NF045027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141012.1"
                     /translation="MVAAVASCQVIFSDDFESGVLGEQWEKYSDDPVRGGIETRPQYV
                     HRGKMSYRISAPAFRGEGRTLDGRVLRESDSWIRTWFLPGYDRTYIRWYAKFAEDFEG
                     RGMHWCQFWACRPDKPRSVLGGAGRRPDGTDRFIVNVEPRGTESMPPPGQIVFYTYWP
                     DMKPSPDGRYWGNYFYPKEPFWIERGRWYCFEMLVKCNQPGKKDGEQVLWIDGKEVLR
                     VDGMRWRDTESLKLNMAMFGNYSSNSQHDRTYWLDDVVISTEYVGPSR"
     gene            complement(5557..5862)
                     /locus_tag="VFQ79_14915"
     CDS             complement(5557..5862)
                     /locus_tag="VFQ79_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141013.1"
                     /translation="MKLAKIGLASFLLGQNLLEDDPEFLTIEAMSTEALTTSEIESAL
                     AATILAKRKSYYDVLEAANKDNEITPWISWFAATAIEAQQRMVAHVEFLIARSCGPG"
     gene            complement(5886..7247)
                     /locus_tag="VFQ79_14920"
     CDS             complement(5886..7247)
                     /locus_tag="VFQ79_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0141014.1"
                     /translation="MNRRTLLKTSAAALGIPAFAQQHRRPNFIVILMDDLGCTDLGCY
                     GARDLKTPNIDALAASGVRFTNWYSNASVCAPARASIMTGRYPAHAGVPTNGPSLPAG
                     QITIARLLKGRGYATGLTGKWHLGTPGNNAPNDHGFDEFYGFHSGCVDFYSHRFYWGE
                     PKRPNFHDLWRNRTEIFEDGQYLTERITANAVDFVQRHRRDPFFLYVAYNAPHYPMHA
                     PAKYVERFRDLPAERRMYAAMISAVDDGVGALAAELDKLNLAEDTFVLFLGDNGATTE
                     PRAGLDFKPATAGSNKPYRGYKFSLFDGGMHVPAIWRWKGKLPAGEVSREVAMSMDVL
                     PTISRIAEAPLPAGYAVDGKDVFTLAAGKARSPHEAIFWSQGEQLAVRRGKYKLVING
                     FTAEDWPEGRKPLAGDDAMFLSDLDADPGESKNLRRLHPALADELATMAHRWKKSLEG
                     GER"
     gene            complement(7258..10521)
                     /locus_tag="VFQ79_14925"
     CDS             complement(7258..10521)
                     /locus_tag="VFQ79_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141015.1"
                     /translation="MSTRWGLLICFVFWSVCAWAQTSTGQITGTVFDTSGAVVPGATV
                     DLKGAETGDLVRTLTTDETGSFTAPLLRPGAYAVEVTVTGFKKLLRSGIQLSVDEVLN
                     LRLTVEPGATSESVTVAASAEMLEEKTNSVGQVVGEHTIQQLPLNGRNYLQLGALTAG
                     AVPSDRSRDRSFAAYGNRGLQNAFLLDGARNQNYLRGLDNRARDAMRPSLEAIAEFKV
                     QTSNFSAEYGASAGAVVNVVTKGGTNEIHGSAFEFLRNSAFDARDFFLPSSSDKPLYI
                     QHQFGGSLGGPVIKNRAWWHGAFQRTHISEGETNISTLPTLSERNGVFTVPVFDPDTT
                     RANPDGAGFVRDPFPGNVIPATRFNPIGKALLERFPAPNLPGTARNYVDNPLQATRAN
                     NATFRGDLRISDHDLLFGRFSFDDGAFLRVPPVPEPAQTGTNRDQPSRSVGIGYTRTI
                     SPSVVNELRFAWNRVAVDQDATLAYDEILPGALAPGVNSSIPTFGLTGYAGLGAQPAN
                     FGNLPLTKSSGVWNVSDNLSMIRGRHTIKAGFDFQVLRITTATTLNGRGNFGFNGVFT
                     QDPLRRPGTGSPVADMLLGLPHTITLGTRSISNERTQNYYWYIQDDWTITPTFTLNLG
                     VRYELTRPFYEANDRLGNLVLDAGGLYGQLILAGDERRPRSLLDTDYSNWAPRVGFAW
                     RTPAPGLVVRGGYGLFYGQDEGYGVTVRMTNNPPFVGFGGVSLTSDQLRPSSTIPLTG
                     TLPERQPEPPRESFLLDPRATAGLVSWSQRYTMPYVQQWSLSLQKELPASLLAEVNYV
                     GNRGTHLWGAFQANQPEPGPGAVNDRRPLAAVTRAPVTRMEPWVSSTYHGLSTRLERR
                     FSSGLSFLAAYTFGRSLDTQSNVDLCDGCVNTAGAGSVQDVRNRRANYGLSDHHIARR
                     LVISGVYELPFGQSKPWLTSGPGRYIFGDWVASGVWSAQDGQPFTLNLNFDNANTGTT
                     NWPDRLRSGRLENPTLDRYYDLDAFVFPAQYTFGNAGRNILIGPGTNNIDFSLQRNFA
                     VPINEGSRMEFRAEAFNLFNRPHFGIPGATIGTPNAGTIGGTSRNNRQLQFGLRLLF"
     gene            10592..11824
                     /locus_tag="VFQ79_14930"
     CDS             10592..11824
                     /locus_tag="VFQ79_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0141016.1"
                     /translation="MSIARAGLGLILMAASLPAIDLHTAARAGDVAQVKALLAAGADV
                     NARDSLGGTPLHDAAWSGATEVVRLLLDAGADTNARHAEAGSTPLHYAVITNHPDIAE
                     LLLAKGADLRARYRTGSTALHLAANRGYTDLVRLLLDKGADVNDSDNAGATALDEAAW
                     KGQADVVKLLIARGARVDVRSKESGATPLHEAAVKGHAEVVAILLASGSDPAARDNNG
                     STALDEALRYRHSKVVEVLLRDGRSGGKPGEVGAGQLQEAILRGQVDVVTLLLDKGAD
                     VNARTRFGSTLLHDAALKGHAELVRLLIARGAKVNARNSTGATPLHDAALGGHREVVR
                     ILIDNGADLNAVDEETGATPLCHAASWGRIQVAELLLERGADLKPRNKAGQTALKAAL
                     DNGNTEIASLLRARGASE"
     gene            11899..13581
                     /locus_tag="VFQ79_14935"
     CDS             11899..13581
                     /locus_tag="VFQ79_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU0141017.1"
                     /translation="MKISVNMVRQVSQLAAAADVLGGQLRRHLVRFAAVVSPHSAALN
                     RAFMTRLRASGYSVKQRSALAAITPGAAANVLSGGRSQAAFFEVVNYNGRRLAKLGLP
                     PSAVSKALAIYDALLRPVLRRGHEKEYPNLLWVREQLQFCTLLTLNSAYYEVREEEAQ
                     SFYRLSRAELESHTVEELLNRCVEILAGFARADRSALYLLSPDRAQLVPGAAPGGKPA
                     SRVPVSPALLRRLQKPRCFAGAKAPGLLLNSAWKKDISTCWSVPLLRAGEIAGVMQFG
                     FRRLYDWLPREQEMLRAAAERAMLAAEKARLMDGLAASEAQIRSLAEHMLHVEEVERQ
                     RISRELHDETGQSLLCIRLQLEMLQQLLGSGDPVLERKAAEIRKLTEKTIVEMRRLIA
                     ALSPSVLQQLGLAAAIRQLGHHLERVKPIRVRLRIGDLPALPKQVQLIAYRILQECCN
                     NILKHSEATSVNILVCFADGRLGLKVEDNGIGFDPGAASAAGDSFGIAGMRERVALLG
                     GAFEIQSRSSKGLRSRRKGTTIGILLPGDLAGRVVTAGRPVPACRTSGITKD"
     gene            13603..14268
                     /locus_tag="VFQ79_14940"
     CDS             13603..14268
                     /locus_tag="VFQ79_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0141018.1"
                     /translation="MAKIRIMLADDHALFRQGIRTLVGSEPDMEVVAEVANGGDAVTK
                     ASESRPDVVLMDIGMPGLSSFEATRQIKKNRPDTKIVFLTMYDDEDYLVEGMEVGASG
                     YVLKDSPSAHLIGAVRDVCRGGSYLSPRMLAQLVDDFRTRIKSTNRTPRFATLTPRER
                     EVLKVLAEGKSVKEIACDLNLSVKTVEAHKFNLMRKLDIHNKAQLVQYAIQKKIIKIP
                     SLN"
     gene            14390..15316
                     /locus_tag="VFQ79_14945"
     CDS             14390..15316
                     /locus_tag="VFQ79_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0141019.1"
                     /translation="MVAHTSRRAVLAMAALPHRLLPQSSQRRAIRLGGPVFLKTDDPV
                     ELAREHRRLGYSAAYCPNAKAGDTARTREIEKAFAAEDVVIAEVGAWVNMLDPDPVKR
                     RENLRFVAERLALAEAVGARCCVDIAGSYNPKVWYGPHPKNLSDEFFDATVENCRRIL
                     DEIKPQRTKFAIEMMGWCLPDGADSYVRLVKAVDRTAFGVHIDVCNGVNSPTRFYNNS
                     GFISECFRNLGLWIVSCHAKDLQWIPEMNIHFVEVIPGRGEMDYRAYLSELSRLEIDA
                     PLMLEHLKTPEEYDEGKRYIQKVANELQLRLA"
     gene            15326..16186
                     /locus_tag="VFQ79_14950"
     CDS             15326..16186
                     /locus_tag="VFQ79_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0141020.1"
                     /translation="MNRREFMTASAAALAAAVPARPAKLPVQKGVIISMLPKELSLAD
                     RMKIARDVGFDVLECLTTPDQKEADAIKAAAEKADLRIHSVMNMAHWKFPLSSADPAV
                     VKTSMEGMETSLKNAHFWGADAVLLVPAVVNPQTSYQDAWTRSQRQIRQLLPMAEDLK
                     VVIAVEEVWNKFLLSPLEFARYVDEFNSPWVRAYFDVGNVVLYGYPQDWIRTLGKRIM
                     KVHLKDFRFKNRVAEFVPLKEGEIQWDEIHKALAEIDYSGPATVELNGGDEAYLREVS
                     RRVDSILSGV"
     gene            16220..>17142
                     /locus_tag="VFQ79_14955"
     CDS             16220..>17142
                     /locus_tag="VFQ79_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEU0141021.1"
                     /translation="MKKFLIGLFSGILLCVLVAVIGFFALLRFSERRPSVASQSTLFL
                     KLDGEVPERPPLEIPVPFLESQTPATTADLWGMFEKAAADPRIKALVLAPRNVQAGWG
                     KLQEIHEGLLKFGKSGKPVIACLQLPGAKEYYLATAAGRIYLSREDILDLKGIRVEAM
                     FFKNTLDKLGVKLEVEHAGRYKDAGDAFTRTSMTPETREVLNSVLDGVYGGLVQTIAE
                     GRNKSPDEIRAAIDEGPFLAGQALSKGLVDALAYEDEVLNELENNLGGSEIRRISHRD
                     YIKATSSGGGGRVVALLVADGTILRGEGSEG"
BASE COUNT         3309 a         5094 c         5358 g         3381 t
ORIGIN      
        1 gtaccggaaa ggggaagggt ttgccggatt tctcgatggc ttgccgcaca tcctggccgg
       61 cgattcggtt cgcgccgtca tagcggccat acaggatgcg cgggaacacg gaaagcccgt
      121 gatctgggct ctgggcgggc acgtcatcaa gtgcgggctg gccccggtgc tgattgaact
      181 gatgtcgcgc cgctatgcaa ccgcgtttgc catgaacggc gccgcttcca ttcacgactt
      241 cgagatcgct cttgctggtc acaccagcga agaggtggaa gctgtcctgc ccgatggcac
      301 cttcggctcg gccgaagaaa cgggtcgtga aatgaatcgg gctttgtcgg cggcagatgg
      361 cattggggcc ggtgaggcgc ttggccgcgc tctcgacgaa ctcgcggatt cgcaattcgc
      421 cgacaaaagc ctgctccttc aggcctggcg ccgctcggtg ccggtcacgg tgcatatcgc
      481 catcggcacc gacacgccgc acacgcatcc ggccgtagac ccggacgcat tggggcgctc
      541 cacgcatcat gattttcgct tgttctgttc gcttgtgtcg gcgctcaacg aaggcggcgt
      601 gtacatcaac tgcggctccg cggtgatcat gccggaggtt tttctgaagg cggtatcggc
      661 ggtccgcaac ctgggaattc tactggccgg gttcaccacc gtgaatctcg atttccttca
      721 acattaccgc ccgcgtgtga acgtggtgga gcgcccgcac atcacggccg gcgggcacgg
      781 ctattccctt accggacacc atgaactgat gctgccgctg attgccgccg cactgattga
      841 gcacgacgca tgagcgatcc ggcaggcggt atgaacggct ctccattccg gcccgcgctc
      901 gtgccgttcg tagaagagac gaatcgcccg tatccgttct ggggctaccg cgatatcgcc
      961 ctcttcctct ccatggcgct gccgagcttt atattggcgc tcctcctgat gaaggtcttt
     1021 ctgagcgtgg cgccgtggta tccgcgcacc aaggccacgg agttgcttcc ggcgcagttc
     1081 ctcgcctacg gactgtggtt cctctcgctg tacctgttgc tgcacttaaa gtacgggcgg
     1141 cctttttggc cttcgctggc ctggcgggtc gtccccggcc agttgcgcaa aagcgccctg
     1201 ctcggcctgg cgctcgcttt cggcgtagcg atcaccggcg gattgctgaa gacgccacag
     1261 atcgagatgc cgttcgagga ttttctgaaa gacccgctct ccatcgcgct ggtagcggtc
     1321 tttgcagtca ctctgggccc gttgttcgag gagttggcgt ttcgcggatt tttccagcct
     1381 ctgctggtcc ggtccgcggg agccatcctg ggcattctga tcgctgcttt gcctttctcg
     1441 ctgctgcatg ggccgcagta tgcctggtcc tggcggcata ttcttctcat cacgctcgcg
     1501 ggctgtgcgt tcggatggca ccggcatcgt accggttcga cggcggcggc ggccatcatg
     1561 cactcggcct acaacctgat gtttgtgacg gcttacattt tgcaagggag gtacgccacc
     1621 ccgtaatggt ggaaacgatt cgatggacgg acgacggcgt agtcatggtg gatcagacgc
     1681 gcctgccgcg ggaggaggtc tttgtcactt gccggactta ccgcgaggtg gcggatgcga
     1741 tccgccgcat ggtaatccgc ggagcgcccg cgatcggcgt ggccgccgcg atgggagtgg
     1801 cgctgggggt gctgcgcgcg gacggcgatg acctcgacgc gcagatggac gagatctgcg
     1861 gcgaactcgc cggcacgcgg cccacggcgg tgaatctttt ctgggccatc cggcgtatga
     1921 aggacgttta cggttccctg cgcggacgtc cgctcgagga gatccgccgc cgcctggtag
     1981 aggaagcgca gcaggttcgc cgcgaggaca tcgccatcaa tgaagcgatc ggccgcaacg
     2041 gcgcggacct ggttcccgac ggcaaaacgg tgctcactca ttgcaacgcc ggcgcgctcg
     2101 ccacggcggg ttatggcacc gccctgggag tgattcgcgc cgcggtggct gccggcaaga
     2161 gcatcgacgt attcgcggac gagacccggc catttcttca gggagcccgc cttaccgcct
     2221 gggagctcca gcaggacggt attcccacca cgctcatcac cgacaacatg gccgggcatt
     2281 ttctgaagtc gggacgcatc ggctgcgttg tcgtgggcgc cgaccggatc gccgcgaacg
     2341 gagacgtcgc aaacaaggtg ggaacgtatt ccgtcgccgt gctggctaaa gaaaacggga
     2401 ttccgttcta tgtggccgcg ccggtttcga ctctggatct ttctctcgct tccggcgacg
     2461 agatcccgat cgaacagcgg gatgcggcgg aagtgaccac ggtgttcggc acgcccgtgg
     2521 ctcccgaagg catcccggtg gagaacccgg ctttcgatat cactccccac cggtatgtga
     2581 cggccattat cacggaacgg ggggtggcgc gcccgccatt cggtgaatcg ctggcgcgaa
     2641 tttccgggag cgataccacc ggaggccgct tttaatttaa cctggagact tatgcgattg
     2701 tttgtggcat tgctggggtg cgccctggcg ctgaacgggg cgggaaagct gtcggggcgg
     2761 agggctccgg gtttttcgct acccgacatg cagatgcgcc agcacgacct tgcagactat
     2821 cgtggcaagg ttgtgctcat cgacatcatg cggaccaatt gcccggaatg ccagaaacta
     2881 acgcccaagc tggagacggt gaaggccaaa taccctggcc gcgtgatcgt gctctcggtc
     2941 gttacgttgc ccgacaacca gaccaccgtc gccaaatatg ttgcggccta caaggtgacc
     3001 ggaccggttt tgttcgattg cggccagatg gctgcgtcgt ttgtcaaagc cactccgcaa
     3061 aatccgagca tgagcttgcc gcacctgttc tttgtagaca cgcagggcgt aattcgcaac
     3121 gatttcagcg atcatgaagt gagcggactg accgtagagg ctctctccgc ggaagtggag
     3181 cggctcctgg taccagcttc gaaatccagg tagccgttgt ccattaagta agattaagat
     3241 taggtataga atctaaacgc cggatacgtt taccatgccc aaggacaccc tgacgataac
     3301 agacaacagg accgggaaga cttacgagat cccgattgaa catgacacga ttcgcgcaat
     3361 ggacctgcgc cagatcaaag tatcggatgg ggatttcggg ctcatgactt acgatccggc
     3421 ttacctcaac acggccgcct gcaagagcac gattacttac atcgacgggg acgcgggcat
     3481 tttgcggtat cggggatatc cgatcgagca actggcggaa cacagcacgt atctggaaac
     3541 ggcttacctg ctttacaggg gagaactgcc caccagccag cagcttgacc gatgggtgac
     3601 cggtgtgacg catcacacca tcccgcacga gaagatcaaa tacttcatgg agggtttccg
     3661 gtacgacgcg catccgatgg gcatctttgt cggcaccgtc gccgcgctgt ccaccttctt
     3721 ccctgaatcg aaacaggtgt tcgatcccga ggtccggcgg atcacgtttt atcgcggcgt
     3781 ggccaagacg ccgacgattg ccgcgttcgc gcaccggtac agcatcggaa tgccgtatgc
     3841 gtatccggac aacgagttgt cctacacggg caatttcctc agcatgatgt tcaaggggac
     3901 ggaactgcgc tacaagccga atcccgtcct cgagcgcgcg ctcgacgtcc tgttcattct
     3961 ccacgcggat cacgagcaga actgctcgac cagtgcgatg cgcgtggtcg gcagcgcgca
     4021 gacggatccg ttcacggcca tcgcggccgc cgcggcggcg ttatacggac ctttgcatgg
     4081 cggcgccaat gaggccgtgc tgcgtatgct ggccgagatc ggctcgaagg acaagatccc
     4141 cgcatacatc aaacgcgtca aagcgggcga agtgaagctg atgggattcg gccatcgcgt
     4201 gtacaagaac tacgacccgc gtgcgcggat catcaagcag acagccgatg aggtcttcga
     4261 ggtgaccggg aagaacccgc tgctcgacat cgcgctggaa ctggagcgca ttgcgctcga
     4321 agacgactat ttcgtcggcc gcaagctcta tcccaacgtg gatttttatt ccggcctcat
     4381 ctaccaggcg atgggattcc cgaccgacat gttccccgtc ctgttcgcta taccgcgcgt
     4441 ttccgggtgg ctgagtcaat gggaagagat gatcctcgat cccgaacaga agatcgcgcg
     4501 gccccggcag gtctacctcg gtgaagggga gcgggactat gtgccgatgg aagagcggga
     4561 agcggtaacg gccacccgtt cctgagcctg gagtcaatgc ttcggacaac aacaaggccc
     4621 cttgccctcg catgtatggt tgccgcggta gcgtcctgcc aggttatctt cagcgacgat
     4681 ttcgagtcgg gtgtactcgg agagcagtgg gagaaataca gcgacgatcc tgtccgtggg
     4741 ggaattgaaa cacggccaca gtatgttcac aggggcaaga tgagttatcg gatctcagcc
     4801 cctgcctttc ggggagaagg ccggactctt gacggacggg ttctccgtga atccgacagt
     4861 tggatccgaa cctggtttct gccgggctac gaccggactt atattcgctg gtatgcgaag
     4921 ttcgccgaag attttgaagg gcgggggatg cactggtgcc agttctgggc gtgccgcccc
     4981 gacaaacccc gttccgtgtt gggcggtgcc ggccgcaggc ccgacggtac ggatcgcttc
     5041 attgtcaatg tggaaccgag gggcacggaa tccatgccgc ctcccggaca gatcgtgttc
     5101 tacacctact ggccggacat gaagccctca ccggacggcc gctattgggg caactacttc
     5161 tacccgaaag agcctttctg gatcgagcgc ggccgctggt actgtttcga gatgctggtg
     5221 aaatgcaacc agcccggcaa gaaagacggc gaacaggtac tctggatcga cggcaaggag
     5281 gttctgcggg tggacggcat gcgctggcgg gacaccgaaa gcctcaaact gaacatggcg
     5341 atgttcggaa actacagcag caacagccag catgatcgta cctactggtt ggacgatgtg
     5401 gtcatcagta ctgagtacgt aggtccatcc aggtagcctc acccctctgg cgcaaagagg
     5461 ccgttctcaa atgccactgg tgttgccggc cgtaggggaa tcatgaggtg ataacgggca
     5521 tgacggcgtt cgccggcacg gactagcgct cctttgctaa ccaggtccgc aagatcgcgc
     5581 gatgagaaac tcgacgtgcg caaccattcg ctgctgtgct tcgatcgcgg ttgctgcgaa
     5641 ccaggaaatc cacggcgtga tctcgttgtc cttgttcgcc gcttcgagta cgtcgtaata
     5701 tgacttccgc ttggcaagga tggtcgcggc cagagcgctc tcgatctccg aggtcgtcag
     5761 ggcctcggtg ctcattgcct caatcgtgag gaattcagga tcatcttcca gaaggttctg
     5821 tcccaagaga aatgaggcca gtccgatttt tgccaatttc aagtcatgag cgatagcgat
     5881 gatttctatc gctcaccgcc ctccagcgat ttcttccacc ggtgggccat ggtcgccagt
     5941 tcatccgcaa gcgcagggtg aagccgccgc aggttcttcg actctcccgg gtccgcgtcg
     6001 agatcggata gaaacatcgc gtcatcgccg gccagcggtt tgcggccttc gggccagtcc
     6061 tccgccgtaa acccgttgat taccagcttg tacttgccgc gccgtacggc cagttgttcg
     6121 ccctgcgacc agaaaatcgc ctcgtgcggc gagcgcgctt tgccggccgc cagggtgaaa
     6181 acatccttgc cgtccactgc gtagcccgcc ggcagcggag cctccgcaat ccggctgatc
     6241 gtcggcagga catccatcga catggcgact tcgcggctca cttctcccgc aggaagcttg
     6301 cctttccatc tccaaatggc cggcacgtgc attcccccgt cgaacaggct gaacttgtat
     6361 ccacggtacg gcttgttgct gccggccgtc gccggtttga aatccaggcc tgcccgcggc
     6421 tccgtggtcg caccgttgtc gcccaggaac agaacgaagg tgtcctccgc gagattcaac
     6481 ttgtccagct ccgccgccag cgcgcccact ccgtcatcta ccgccgagat catggccgca
     6541 tacatccgcc gttctgccgg caggtcgcgg aagcgctcga cgtacttcgc cggtgcgtgc
     6601 atcggatagt ggggcgcatt gtaggcgacg tacaggaaaa acggatcgcg gcggtgccgt
     6661 tgcacaaaat ccaccgcgtt agcggtgatg cgttccgtga gatactgccc gtcctcgaag
     6721 atctcggtgc gattgcgcca gaggtcgtga aagttaggcc tcttcggctc gccccagtaa
     6781 aaccggtgcg agtagaagtc gacacagccg gaatgaaagc cgtaaaactc gtcgaagccg
     6841 tggtcgttcg gcgcattgtt ccctggcgtg ccgagatgcc atttgcccgt cagtccggtc
     6901 gcatacccgc ggcctttcaa cagccgcgca attgtaatct gcccggccgg cagtgacgga
     6961 ccgttcgtcg gtacgcccgc atgcgccgga taccggcctg tcataatcga cgcgcgcgcc
     7021 ggagcgcata cgctcgcgtt cgaataccag tttgtgaagc ggacgccgga agccgccagg
     7081 gcatcgatat tcggcgtctt cagatcgcga gcgccgtaac agcccaggtc cgtgcagccc
     7141 aggtcgtcca tcaggatcac gatgaagttg gggcgccggt gctgctgtgc aaatgccggt
     7201 atccccaggg ccgcagccga agttttcagg agtgttcgcc ggttcataga gcctccgtca
     7261 gaatagcagc cgcaggccga attgcagttg ccggttgttt cgcgacgtac cgccgatcgt
     7321 accggcatta ggcgttccga tcgtcgctcc ggggattcca aaatgggggc ggttgaagag
     7381 gttgaaggct tcggcgcgaa actccatccg cgagccttcg ttgatgggca ccgcaaagtt
     7441 gcgctgtaga gagaagtcga tattattcgt gcccggaccg atcagaatat tgcgccccgc
     7501 gttgccgaat gtgtactgcg cgggaaagac gaacgcgtcc aggtcgtagt agcggtcgag
     7561 cgtagggttc tcgagccgtc cgctgcgaag ccggtcgggc caattcgtcg taccggtgtt
     7621 ggcgttgtcg aagttcaggt taagcgtgaa cggctggccg tcctgcgccg accacacgcc
     7681 gctggctacc caatcgccaa aaatgtagcg gcccggtccg ctggtgagcc acggcttgct
     7741 ttggccgaac ggcagctcgt aaacgccgct tatcaccagc cgccgcgcga tgtggtgatc
     7801 cgagaggccg tagttggccc ggcggtttct cacgtcctgt acacttcccg cgcccgctgt
     7861 attcacacat ccgtcgcaca ggtcgacatt gctctgcgtg tcgagggaac gtccgaacgt
     7921 ataggccgcc aaaaaactca agccgctgct aaagcgcctt tcgaggcgcg tcgacaggcc
     7981 atggtacgtg ctgctgaccc acggttccat gcgcgtaacc ggtgcgcgtg tgacggcagc
     8041 gagcggcctg cggtcgttga ccgctcccgg tcccggctcc ggttggttag cctgaaacgc
     8101 tccccatagg tgcgtgccgc ggttgccgac atagttcact tccgccagca gactggccgg
     8161 cagctccttt tgcaggctca ggctccactg ctgcacgtag ggcatcgtgt agcgctgcga
     8221 ccagctcacc agaccggctg tcgcgcgcgg gtcgagcagg aagctctcgc gaggtggctc
     8281 cggctgccgc tccggcagcg tgcctgtcaa cggaattgtg ctcgacggcc tcagttgatc
     8341 gctcgtgagc gatactcctc cgaagcccac gaacggagga ttgttcgtca tccgtaccgt
     8401 cacgccgtag ccttcatcct ggccgtagaa gaggccgtat ccgccgcgaa ccaccaggcc
     8461 cggagccggc gtgcgccagg cgaaacccac gcgcggagcc cagttgctgt aatccgtatc
     8521 gagcaggctg cgaggccgcc gttcgtctcc tgcgagaatg agctggccgt acaatccgcc
     8581 ggcgtcgaga accaggttgc cgagccggtc attcgcctca tagaacggcc gcgtcagctc
     8641 atagcggact cccaagttga gcgtgaacgt tggcgtgatg gtccaatcgt cctggatata
     8701 ccagtagtag ttctgtgtcc gctcgttgct gatgctgcgt gtgcccagcg tgatcgtatg
     8761 cggcaggccc aacaacatgt cggcaaccgg cgatcccgtg cccggacggc gcaacgggtc
     8821 ttgcgtgaag accccgttga atccaaaatt tccgcgtccg ttgagcgtag tggccgtcgt
     8881 aatccgcagc acctgaaaat cgaacccggc cttgatcgtg tgccggccgc gtatcatcga
     8941 aaggttgtcc gagacgttcc acacgcccga gcttttggtc agcggcaggt tgccgaaatt
     9001 tgccggctgc gcgccgagtc ccgcatagcc cgtcaagccg aacgtcggga tgctgctgtt
     9061 cactccgggc gcgagcgctc cgggaaggat ctcgtcgtag gcgagcgttg cgtcctggtc
     9121 gaccgcgacg cggttccatg caaagcgcag ctcgttgacc acgctcgggc tgatcgtgcg
     9181 tgtatagccg atccccacgc tcctcgatgg ctgatcccgg ttggtcccgg tttgcgcagg
     9241 ttccggcacg ggagggactc tcaggaacgc accgtcgtcg aacgaaaacc ggccgaataa
     9301 cagatcatgg tcgctgatgc gcagatcgcc gcggaacgtg gcgttgttgg cgcgcgtagc
     9361 ctgaagcgga ttgtcgacgt agttgcgcgc ggtgcccggc agattgggag ccggaaaacg
     9421 ttcgagcagc gctttgccaa tcgggttgaa acgcgtcgcc gggatgacgt tgccagggaa
     9481 cggatcgcgg acaaacccgg cgccgtccgg attcgcgcgt gtcgtatccg ggtcgaatac
     9541 cggaacggtg aagacgccgt tgcgttcgct gagcgtcggc agcgtggaga tattcgtttc
     9601 cccctcgctg atgtgcgttc gctgaaacgc gccgtgccac cacgcccggt ttttaatgac
     9661 gggaccaccc agggacccgc cgaactgatg ctgtatgtag agcggcttat cgctcgaaga
     9721 cggcaggaag aaatcgcgcg cgtcgaaagc gctgttgcgc agaaactcga acgccgagcc
     9781 gtgaatttcg ttggtgcctc ctttggtcac aacgttcacg accgcgccgg ccgatgcgcc
     9841 atactccgcc gaaaaattgc tggtctgtac cttgaactcg gcaatggctt cgagagaggg
     9901 ccgcatcgcg tcgcgcgcgc gattatccag gccgcgcagg tagttctggt tccgcgctcc
     9961 atccaacagg aatgcattct gcaatccccg gttgccatag gccgcgaaac tacggtcgcg
    10021 cgaccggtcg ctcggaacgg cgcccgccgt gagcgctccc agttgcaggt agttgcggcc
    10081 gttgagggga agctgctgaa tcgtgtgttc gcccaccacc tggccgacgc tgttcgtctt
    10141 ctcttcgagc atctcggccg aagccgcgac ggtcacggat tccgaggtgg cgccaggctc
    10201 gaccgtcagt ctgaggttga gtacctcgtc gacgctgagc tgaatcccgg accgcagtaa
    10261 tttcttgaaa cccgttacgg tcacctcgac ggcatacgcg cccgggcgta gcagcggcgc
    10321 cgtgaaactg ccggtttcat ccgtggtgag tgtgcgcacc aggtcgcccg tttcggcacc
    10381 cttgaggtcc acggtcgcac ccggcaccac cgccccactc gtgtcgaaaa ccgtccctgt
    10441 gatctgaccg gtcgacgtct gcgcccaggc gcagacggac caaaacacaa agcagatgag
    10501 gagcccccag cgagttgaca tgccactatt tcatcactgt tttcgatcac ccgccggcgt
    10561 gttggtgcgt gcggcccggt aaactgtagg tatgtcgatc gcccgcgccg gtcttgggct
    10621 gattctaatg gcggcgtccc ttccagcgat tgatttgcac accgccgccc gcgccggcga
    10681 tgttgctcag gtgaaggctc tgcttgcggc gggggccgac gtcaacgcac gcgactcgct
    10741 cggcggcacg cctctgcacg atgcggcatg gagcggggca acggaagtgg tccggttgct
    10801 gctcgacgcg ggcgccgata cgaatgcgcg tcatgcggag gcaggttcca ctcccctgca
    10861 ttacgcggtc atcacgaacc atccggacat tgcggaattg ttgctcgcaa aaggcgccga
    10921 tctgcgcgca agataccgca ccggatcgac ggcgcttcat ctcgccgcca accggggtta
    10981 cacggacctg gtacgcttgc tgctggacaa gggtgcggac gtcaacgaca gcgacaacgc
    11041 gggggccacc gcgctcgatg aggcggcctg gaaagggcag gcggatgttg tgaagctgct
    11101 gatcgcccgg ggcgcgcggg tagatgtaag gagcaaggaa tccggcgcca cgccgctgca
    11161 tgaagcggcg gtgaaggggc acgccgaagt ggttgctatc ctgctggcct cgggttccga
    11221 tccggctgcc agggacaata acggttccac cgcgcttgac gaggcgctgc gttacaggca
    11281 cagcaaagtc gtcgaggttc tgctgcggga cggccggtcc ggaggaaagc cgggtgaggt
    11341 cggggcgggc cagctccagg aagcgattct gcgcgggcag gtcgatgtgg tgacgctgtt
    11401 gctcgacaag ggcgccgacg tgaatgcgcg cacccggttc ggctccacgt tgttgcatga
    11461 tgctgctttg aagggacacg ccgaactggt gcggctgttg atcgcgcggg gcgcgaaagt
    11521 gaatgcgcgc aactcgacgg gtgccacgcc cctgcatgat gcggcactcg gcggtcatag
    11581 ggaagtcgtt cggattctta tcgacaacgg cgcggacctc aatgccgtgg acgaggagac
    11641 cggggcgaca cccctctgtc atgcggcttc gtggggaagg atccaagtcg cggagttgct
    11701 attggaacgc ggcgccgact tgaagcctcg caacaaggcc ggccagaccg ctttgaaggc
    11761 cgcgctggac aacggcaaca ccgaaattgc ctccctgctc cgcgcccggg gcgctagcga
    11821 atgaactagt gcgaatgccc gaggctgccg gcccggcgag caccggattt tctctaaagg
    11881 gttcccctaa tttggccgat gaagatatcg gtgaacatgg tgaggcaagt gagccaactg
    11941 gcagcggcgg cggacgtcct cggcggacaa ctgaggcgtc atctggttcg ttttgcggcc
    12001 gtcgtttcgc ctcattcggc cgctctgaac cgtgccttta tgacccggct gcgagcgagc
    12061 ggatatagcg taaagcagcg cagcgcgctg gcggcgatca ctcccggcgc ggcggcgaat
    12121 gtcctgtccg gcgggcgctc ccaggcggct ttctttgaag tggtgaacta taacggccgc
    12181 cgtctggcca agctcggctt gccgccaagc gccgtatcca aggctctcgc catttacgac
    12241 gcccttcttc gccccgtgtt gcggcgcgga cacgaaaagg aatacccgaa cctgctgtgg
    12301 gttcgagagc aactccagtt ttgcacgctg ttaacgttga actccgcgta ttacgaggtc
    12361 cgtgaggaag aggcacagtc gttttaccgg ctatcgcgcg ccgagttgga atcgcatacg
    12421 gtcgaggaac tgctgaatcg atgcgtggag atactggcgg gctttgcgcg tgccgaccgc
    12481 tctgctttgt acctgctgtc accagaccgc gcgcagcttg tgcctggggc ggctcccggc
    12541 ggcaaaccag cctcccgggt gcccgtttct ccggcattgc tccgccggct tcaaaagccg
    12601 cgatgcttcg ccggtgcgaa agcgccgggc ctcttgctga atagcgcatg gaagaaggac
    12661 atttcgacgt gttggtccgt gccgcttctg cgggccgggg agatcgccgg tgtgatgcag
    12721 ttcggcttcc ggcgcctgta tgattggctc ccacgcgagc aggaaatgct gcgagcggct
    12781 gccgagcggg cgatgctggc ggccgagaag gcccggttga tggacggctt ggcggcgagt
    12841 gaagcacaaa tacgcagcct ggcggaacat atgctccatg tggaagaagt ggagcggcaa
    12901 cggatcagcc gcgaactcca cgacgagacc ggacaatcgc tgctttgcat tcgtcttcag
    12961 ctcgaaatgt tgcagcagct tcttgggtcc ggagaccccg tactcgagag aaaggccgcg
    13021 gaaatccgca aactgacgga gaagaccatt gtggaaatgc gcaggctgat tgccgcgctg
    13081 agtccgtccg ttctccagca acttggattg gccgccgcca tccggcagct tggacatcac
    13141 ctggagcgtg tgaagccgat ccgggtgcga ttgcgaatag gagatctgcc cgcccttccg
    13201 aagcaagtgc aactgatcgc ctacaggatt ctccaggagt gttgtaacaa catccttaag
    13261 cactcggaag caaccagcgt aaacattctc gtctgctttg ccgatggaag gctgggactg
    13321 aaagtcgaag acaacggaat cggcttcgat cctggagcag cctcggccgc gggcgattcg
    13381 tttggcatag cgggaatgcg tgagcgggtc gcgctactcg ggggtgcatt cgaaattcag
    13441 agccggtcct ccaagggcct gcgttccaga cgaaagggaa cgaccatcgg cattctgttg
    13501 cccggggatc tcgcgggcag ggtggtaacg gcgggacggc cggtaccggc atgccggaca
    13561 tcgggaatta caaaggactg atacaggagc agggcggtac ttatggccaa aatacgaatc
    13621 atgttagcgg acgaccatgc gttgtttcgc cagggaatca gaacgctggt cgggtcggag
    13681 cccgacatgg aggtcgtcgc cgaggtggcc aatggaggcg acgcggtaac gaaagcttct
    13741 gaatcgcggc cggacgtcgt tctgatggat atcggaatgc ccggtctgag cagcttcgaa
    13801 gcgacgcgcc agatcaaaaa gaatcgcccg gacaccaaga ttgttttcct tacgatgtac
    13861 gacgacgagg attatctggt ggaagggatg gaggttggcg ccagcggata tgtcttgaag
    13921 gacagtcctt cggctcattt gattggcgcc gtccgggatg tttgccgcgg cggcagttat
    13981 ctcagcccac gcatgctggc gcaattggtg gacgacttcc ggacacgcat caagtccaca
    14041 aaccggactc cgcgattcgc cacgctaacc ccacgcgaaa gggaagtact gaaggtgctg
    14101 gccgagggca aatccgtcaa ggagatcgcc tgtgacctga acctgagcgt caaaacagtc
    14161 gaagctcata agttcaactt gatgcgcaag ctggacattc acaacaaggc gcaactggtt
    14221 cagtacgcca ttcagaagaa gatcattaag atcccgagcc tcaactaaac gcgacgccac
    14281 tccctggcgg tcgtggcgcg gaaacgaata ctgagccgcg accgcgagga agcgcagttg
    14341 gatggccgtg cccgtagctg cggccgggta atccctgtac aatcttttcg tggttgctca
    14401 tacttcccgg cgtgcagtgc tggccatggc ggcgctaccc catcgcctgc ttcctcaatc
    14461 ttctcaacgg cgagccatcc ggcttggagg acccgttttt cttaaaaccg acgatccggt
    14521 ggaactggcc cgcgagcatc gaaggctcgg ctacagcgcc gcatactgtc caaacgccaa
    14581 ggctggagac acggcgagaa cacgcgaaat cgaaaaggcg ttcgcggcgg aggacgttgt
    14641 catcgcggaa gtcggcgctt gggtcaacat gctggatccg gatcccgtca aacggcgcga
    14701 gaacctccgt ttcgttgccg aacgcctcgc cctcgcggaa gcggtcggtg cgcggtgctg
    14761 cgtggacatc gccggttctt acaacccgaa ggtgtggtac ggtccccatc ccaaaaatct
    14821 ctccgacgag ttttttgatg cgaccgtgga aaactgccgc cggattctcg atgaaatcaa
    14881 gccgcaacgc acgaaattcg ccatcgaaat gatggggtgg tgccttccgg acggtgcgga
    14941 ttcctatgtc aggctcgtaa aggctgtcga ccgcaccgca ttcggcgttc acatcgacgt
    15001 ctgcaatggt gtgaactcgc caacccggtt ctacaacaac agcggcttca tttcggagtg
    15061 tttccgcaat cttggcctct ggatcgtttc ctgccacgcc aaagatctcc agtggattcc
    15121 cgaaatgaac atacacttcg tcgaggtgat ccctggccgg ggagagatgg actaccgggc
    15181 gtatctctcc gaattatccc ggctggagat cgatgcgccg ctcatgctgg agcatctgaa
    15241 gacacccgag gaatatgatg aagggaagag gtacattcag aaagtcgcaa acgaactcca
    15301 actccggctc gcataaagga atcgaatgaa cagacgtgaa ttcatgactg cctcggccgc
    15361 cgcacttgcc gccgccgttc ctgccaggcc cgcgaagctg cctgttcaga agggggtcat
    15421 catcagcatg ctccccaaag agctgagcct cgccgacaga atgaagatcg caagagacgt
    15481 gggcttcgac gtcctggaat gcctcaccac gcccgatcag aaagaagccg acgccatcaa
    15541 ggctgccgcg gaaaaggcgg acctgcggat tcattcggtc atgaacatgg cgcattggaa
    15601 attcccgctg tcttcggcgg acccggccgt cgtgaaaacc agcatggaag ggatggagac
    15661 cagcctgaag aacgcgcact tctggggagc ggacgccgtg ctgctggtgc ccgccgtcgt
    15721 caatccgcaa acatcctatc aggacgcctg gacgcgctcc cagcggcaga tccgccagct
    15781 cctcccgatg gcggaagatt tgaaggttgt gatcgcggtg gaagaagtct ggaacaaatt
    15841 cctgctcagc ccgctcgaat tcgcgcggta tgtggatgag ttcaacagcc cgtgggtaag
    15901 ggcctacttc gatgtcggca acgttgtatt gtacggctat ccgcaggatt ggattcgtac
    15961 gctcggcaag aggatcatga aagtgcacct gaaggatttc cgcttcaaga accgggttgc
    16021 cgaattcgtg cccctgaaag agggagagat tcagtgggat gaaatccaca aggcgctcgc
    16081 ggaaatcgat tattccggac cggccacggt ggaattgaac ggtggagatg aggcgtacct
    16141 tcgcgaggtc agccggcggg tcgactccat cctttccgga gtttgaccgg cttgtccttt
    16201 ataaagcgtc tgtaatggca tgaaaaagtt tctgattggt ttattcagcg gcatcctgct
    16261 gtgcgtcctg gtggcggtaa tcggcttttt cgccttgctg cgattcagcg aacggcgtcc
    16321 atcggtcgct tcgcaatcca cgctgttttt gaagctggat ggtgaagttc ctgaaaggcc
    16381 tccactggaa atcccggttc ccttcctgga gtcgcagacg cctgccacca cggccgattt
    16441 atgggggatg ttcgaaaaag cagcggcgga tccgcgcatt aaggctcttg tcctggcgcc
    16501 gcgaaacgtc caggcaggtt ggggcaagct ccaggagatt cacgaaggat tgctgaaatt
    16561 cggaaagtcc ggcaagcccg tcatcgcatg tctgcaactt cccggagcga aggagtatta
    16621 cctggcgacc gccgccggcc gcatctacct gtcccgcgaa gatatcctcg acctcaaggg
    16681 cattcgggtg gaggcgatgt tcttcaagaa caccctcgat aagctcggcg tgaagctcga
    16741 ggtggagcat gccgggcgct acaaggatgc gggcgacgcc ttcacccgca cttcgatgac
    16801 tcccgaaacg cgcgaagtgc tcaactccgt gctcgatggc gtgtatggag ggctcgttca
    16861 gaccattgcc gagggcagaa acaaatcccc cgacgagatc cgggccgcca tcgacgaagg
    16921 cccgttcctc gccggacagg cgctgtcaaa aggcttggtg gatgccctgg cctatgaaga
    16981 tgaggttctc aacgagttgg aaaataatct cggtggatcc gagatccggc gaatctctca
    17041 ccgcgactac atcaaggcca cctcctccgg cggcggcggg cgcgttgtag cgcttctggt
    17101 ggcggacggc acgatcttgc ggggcgaggg ttccgagggg at
//
LOCUS       DASTMK010000344        46332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_529481, whole genome shotgun sequence.
ACCESSION   DASTMK010000344 DASTMK010000000
VERSION     DASTMK010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 46332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46332
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529481"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(438..2756)
                     /locus_tag="VFQ79_14960"
     CDS             complement(438..2756)
                     /locus_tag="VFQ79_14960"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HEU0141022.1"
                     /translation="MASRAVIATEPQTSTVYASSKPLAPGDYVWQAVYLDANGAPTGA
                     SRSRTFKLSESLPLLPMPDVAALKTKLASVRPRLFLTANRTAEIKAAVASGNVRWWKP
                     MLQASDAALEETLYPEPAGYPTAEFNVDDWRRIYTPGKIGSAHVARLALAYRITGEEK
                     YLEGARRWLLHLASWDPRGSTSHDVPQPNGSSGNDEASMPMLDRMSFAWDWIGDKLTP
                     EERKKVLASMTERGNQVLALLRTQDFLSHPFSNHEGRVLAFLGNAGLAFLGDIPEAEQ
                     WLDYVLRCYLTSYPGWGGDQGGWAQGVSYWSSYVYYLTTFAEALRGVTDVDLFRRPFY
                     QNTGYFAVYFQPAYAPRGAFGDGAERGPGESQQVMLERFADAFDDPVLRWNAQAIPVP
                     PPSIAENKWHEWAIEDVGSVLRARANGAPGKAQPPSELDGSRYLSDIGWVAMHSALGD
                     AANDVWALFKSSRFGSFSHSHADQNTFQLNAYGRALLIDSGYYPWYGSAHHGLWTRQT
                     RAHNGILINGRGQPTHRWEASGSIEHYERKGLITLARGQAASAYNLQQTPGMLKMWQK
                     HMKEPVPPLDPEAKTFERTLAFVGSKERPVLIVHDYLVTAKPATFGWLAHALNQMETD
                     SQTGTIYVRDGDARLAVRLVSTVPFRFSQTDRFSVAPEVSQSSGCAASPKDCAARFPN
                     QWHLSARTTQPAAEAKFLAVMVPYRASEPQPQISAEKSGDAWILRVNDTVAGAWFGAG
                     RLGKILLESAPSEARLVVQTAEGDRTDTVVSQ"
     gene            complement(2738..2977)
                     /locus_tag="VFQ79_14965"
     CDS             complement(2738..2977)
                     /locus_tag="VFQ79_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141023.1"
                     /translation="MHNPIFAILFALAVLAGCGEQSRPWTDRIAVVEWQPNPQDLIAY
                     PADGQSAGVNPPGFTWTPNEKAKTRFAALMWLPAR"
     gene            complement(3105..4742)
                     /locus_tag="VFQ79_14970"
     CDS             complement(3105..4742)
                     /locus_tag="VFQ79_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141024.1"
                     /translation="MYNEPSEHSGFTGVYSNFPYADFLLGIPQTSNRTNPAEQMYHLS
                     NTDVAGFIQDDWKVTRRLTLNIGLRWDFNPPYHERNGLSFHFDPTSGRVVVPSEESQR
                     HVNPLFPANLAPIVTARDAGLPGNLFYSYKKHFMPRFGFAFRPFADSRTVIRGGYGMY
                     TDQITDAAWNNIVGGPYISDETFTNAIVGGKPNFMFPLAFPDGFGATGVQNFTANDPH
                     LRSPVIQQWNVTVERELFDMGVRVSYIGTNSRHLIWTANLNQPAPGMEPFRNDMRQFP
                     AFRTVALRRNGGNHDYHSLHVVAERKMKNGLYYQFGWTWAKALTDVLSDGEPGSTPEN
                     SYARRMERANADYIPRHRTVTNLNYELPFGPGKPWLSAIRGVPKFILGGWSLTAMLMT
                     DTGLYFNPVFSGYDVSNTNTIGGRPDRIADGNLPKAERSVSMWFDPAAFRVPGDLDGD
                     HRPDVAVGRFGNSSPNVLQGHGLFHLNAGLFKRFFISERVTAVLQGTFDNALNHPGWG
                     IPSNNISVPGEVGQIRSLRARGGGGPRNGMVALRIEF"
     gene            complement(4639..6360)
                     /locus_tag="VFQ79_14975"
     CDS             complement(4639..6360)
                     /locus_tag="VFQ79_14975"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141025.1"
                     /translation="MRVFRLIFLFSFVTIAALSQTTLGTILGETTDSSGALLPDVAVV
                     VRNMATGIEHHATTGANGLYQVTNLPPGRYSISVSRTGFRTHVISDVILETSATVRTD
                     AVLAVGELATQVTVEASAPLINTEGAEVANVRSAKIILEAPVNYRGDSTSGYYSSVLH
                     LTPGAQRAQGSNFSLAGARGRQYGTTVDGTKIESFSAQGAMDSTAEMRVQLANDKAEA
                     LRPGGMYASSKSGGNDLHGAVFYYLGSSRLRARSPFSLTVPFRDDDDYGAAVGGPIIK
                     NRTFFHSMYERFPMRMETVLNTSVPTMRMREGDFSALLPGRVIRDPLNGQPFADNVIP
                     QDRLNPVSLKVQERFYPLPNSGTPGSYQSNFRDTLPSKWFKTQVETRIDHKLTNENSM
                     FGRVMWNRGGWESQSEAVPTFPYTRGVRSTTVVTISDTHIFTPSLINEFRFGTLRIQY
                     PYFNPLNGADLIREFGLEGLSPDVTIQTGAPRFNVTGFASLGAATMVSDDRERHVQFS
                     DNVTWIRGVHTVKAGFDIRHNTRANFVGGGGFVQRAFGTFRVHGRLQQFPVRRLPARD
                     PPDVESY"
     gene            complement(6574..7638)
                     /locus_tag="VFQ79_14980"
     CDS             complement(6574..7638)
                     /locus_tag="VFQ79_14980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0141026.1"
                     /translation="MGSLRIVETGIINADDAAYPSLIRLDDGEILAAFTVGGGPQALG
                     GTALARSCDGGRNWQTESMILSPSDSPPTTNSLRLSRTPQGTLIAYGFRKFSQNGDRS
                     FGHRLRRDPVYTLSLDGGRVWGPVQTIPTSAGDAWEVSNPMVVLSGNRWLAPAASLFH
                     PDRLGETALVFCSDDGGVSWPDSTIVFHDPEGVRGFFEQKIIETEPGRLLATAWTVRM
                     GDYRDLDNHFSVSTDAGRTWSPARPTGIQGQTLTPIWLGGDRIAALYNCRYGRQGVRM
                     CLVRYTAELWTVEWEEMLWDARASRSVTLSTSGLEELSTFAFGLPCVIRLDARLLLAA
                     HWCVEDGVCLIRWTRLELLP"
     gene            complement(7631..9088)
                     /locus_tag="VFQ79_14985"
     CDS             complement(7631..9088)
                     /locus_tag="VFQ79_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEU0141027.1"
                     /translation="MTLLDHIVLTIYFCTVVLIGTRFSAGQKTLQEFFLGNRNIPWWA
                     ASLSGVATVLSAVAYLGAPGQAYKADLTYLQYRLALPFAILLVCGLMIPYFYRMQVFT
                     VYEYLERRFDLKTRLLGSAIFLVLKCCYMGVVIYAPSVFVAEITGFSLPFVIVVTGVL
                     TALYTTLGGMRAVIWTDTFQLFVLLGGLTAAALVIGIRLDGGWSEVFAVAGERQKLRF
                     FDFSPSLTTEFTFLGGLIGGIFLMVSEYGTDQASVQRFLTTSSMRRGQWAVGSSMVIS
                     SCLGALLFLVGTGLYVYYLEFADKGGIGLAPDRVFPKFIAEELPTGFRGLLVASVLAA
                     AMSTISGVLNSLTTVVMVDFGQTLLQRTSSVASARRVTFGLGICCTLFALFADRLGNL
                     IVASAKLTSFFGGSLVGIFLLGMTARHVGGNAAFFGLLVGSAAVAVLSASTAVSWMWY
                     GAFSALTAYVAGLLFHLIEPRNTVKSVEQRETVRG"
     gene            complement(9106..10515)
                     /locus_tag="VFQ79_14990"
     CDS             complement(9106..10515)
                     /locus_tag="VFQ79_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141028.1"
                     /translation="MPIEVQAGQPQLFVDDALIESYAGVRRTLHPARKLDRPVVEPDK
                     PWEGKRVNLFGTVLYDPESKLFRMWYNGALHKSAYPEMKALRDPVCYATSKDGIHWDK
                     PALGLIEFAGSKHNNIVYDIHSPSVVIDRRESDPNKRYKMIGVSVARHRGLPSSAHGY
                     WAAYSADGLRWHAYPINPVLPHSDTISMVQHPRTGEFLAFHKRPGKVRGLDRRLVWLS
                     ASKDFQTWSEPKLILPPDEQDDAWAKTPEQRMEWYNMSGIPYGSHFLGFITAFRMEKV
                     WKRDEVGPDQSPHDGPIDVQLTYSRDGYTWKRFEDRSPIIAIGKPGTFDGGMLLGVGS
                     TLVSQGDEVHLYYTGYTATHGAPMRDKVATIGRATWRMDGFVSMDAAAAGGTVTTKLL
                     KFDGHRLEVNAAAAPGSIRVELLDDTGKRLDEAALITGDRVAHPVRWKSNTPLSTAKP
                     VRLRFTLKEAQLYSFRVLP"
     gene            complement(10633..13875)
                     /locus_tag="VFQ79_14995"
     CDS             complement(10633..13875)
                     /locus_tag="VFQ79_14995"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0141029.1"
                     /translation="MKLSTRRLVPAALIALLLLTRPVYPQGTAARLIGFVSDPSGAAV
                     VGTTVAVRNVATNLERSVQTNETGDYTVANLPPGVYSVIIQHGGFKTFESSAELAVNQ
                     TARLDAQLSVGETAETVTVEASVPLVDSETAALGQVIGATQVRELPLNGRNFVQLGQL
                     VPGATPGPPAATTVRTRQGGHSLTINGQRQDQNNWMLDGVDNNAQLFGMVVVVPSIES
                     ISEFKVQTSTYSAEFGRAAGAVVNVETKSGSNEFHGSAYEFLRNDNFDATEYFAPVAP
                     ETGRRAKPPLVFNQFGASLGGPISRNNTFFFVNYEGRRTRRSAATGSLAPTAAQRTGN
                     YAGSGIIYDPLTYTPQSPVRQPFAGNQIPSNRIHPASKSILEYIPLPNTDDSARNFTT
                     IATRTDDSDQVPVRVDQKLSDKQWLTGRFSYYNTDNVTNNAFPLDADILRNRHRSFAA
                     QHTYSLTPTLINEFRAGMNRYHFLFLHETAGENFTDMLNLPSLAKGTIADGFPDVRIS
                     GLARLGGNAAVPLDREETTIQLTDSVSWIRGAHSLRVGGDARRYTANNFQPQWGRGRY
                     TFSGVFTGPVGGRHTDGFADFLLGLPSAQNLSDVSRFHGQQPRNLRWSAFVQDDWRVS
                     SKLTLNLGLRFERDGAWKEKFNQVGSFDPATGQMVYAPDYEMPFALPYPNRQGDSNDL
                     QAATNGFAPRVGLAWRPLRNASFVVRSGYGIFWAARTAENLLFSAAVPPFAIDDLQSS
                     GSVIPEIQFGSTRLGDPQQLIPAVLSTSVVPLGTGRNPYTQQWSLTLEKQLGANIGAS
                     VGYVGNRTLRLQRSYNGNSARPGPGTLQPRRRYPLFGALSFHDSDGSSNYHALQAKIE
                     KRFSRGMNLLSTYTWSKAIDDHGGEAESAGGGIQDPDNRAAARGLAGFDLRHRFTLAA
                     VYELPVPDLGNRAANLALRGWQVNAVANIQSGFPFTPLVGGDSVNAGAGAVHPNVSGS
                     SNGNLPDGERSIERWFDTRAFTAPAQYNFGNAGRNILIGPGTQLIDLGLARNFAIHEE
                     HRLQFRFEMFNALNHPNFGFPGSTLGTANFGVIRTAGRPREIQMALRYSF"
     gene            14014..14709
                     /locus_tag="VFQ79_15000"
     CDS             14014..14709
                     /locus_tag="VFQ79_15000"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0141030.1"
                     /translation="MGTSTFGKLKQESTSDRIYAAIREAIFSGRLKTGQRLTEIQMAK
                     EFEVSRASLREALQRLSHEGLVELNSYKGARVIELTPEQLDEMLALRLLLEAEAVRQA
                     KRRLTQTDRDVLYAIARKLHNPGVNPRAYPTFDFELHQTIWEISGNRTLQRHLSLLVS
                     PLFAMGTILRYSESSRDRSDSTRDAGVDHISLVEAICDGTEERAIELMQRHILDNWKV
                     TRSRIESFMKQDS"
     gene            14739..15641
                     /locus_tag="VFQ79_15005"
     CDS             14739..15641
                     /locus_tag="VFQ79_15005"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0141031.1"
                     /translation="MQKLKGVIVPVATPVDSAGDPDLAALRRLLSFLLAHSLDGLFAN
                     GSMGGFAHLTDASQYQIIETIAAQPRGVPVVAGVSDTSISRVRQKIAVIQTMPVAALV
                     LLPPYYYMCRQDELIRFFLLAADFAQKPIILYDNPRYTQNALEPATIAGLARHPNIHG
                     IKISAPDVLKWERVLEHELPRDRFSLVCGAEKLMDVALGMSFDGITGGLHNITPGLAV
                     QLYRAASSGDAATASRLQAQLNRIYQVFEADGGWRGLEVAFAALSMPALFAPPPHDIP
                     VPEEKRRGILEILESENVLAANPA"
     gene            15667..17271
                     /locus_tag="VFQ79_15010"
     CDS             15667..17271
                     /locus_tag="VFQ79_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0141032.1"
                     /translation="MLIVGGGAGGCAAALAVCEAGRRVILSEESPWIGGQFTSQGVPA
                     DEHGWIEQFGCTARYRRFRNSIREYYRSHYPLTHFARLHPYLNPGNGWVSPLCAEPRC
                     VLAVLESMLEPYEVAGHLRILRHHSIASLDIDNDRIRAALLYDSETGVRREIEARFFL
                     DASELGDVLPLAGVEHVSGVESQAMTGEPGAPLQPEPRSMQAFTWCFAVDYLSDEDHT
                     IERPRDYDFWRDYRPRLSPPWPGPLFQLAGLNPRTLAPVSYTFNPHGESGLAFSGLWT
                     YRRLLDRMQFAPGSVASDICLVNWPMNDYMEGDLLGSAVECANHLHRAQQQSLSLLYW
                     LQTEAPRPKGGYGWRGLRPRGDVLGTATGLALRPYMRESRRIRAIRTVTEVDVSAVHR
                     PGATRAIHCRDSVGVGYYRIDLHPCTGGHNYIDVPALPFQIPLGSLIPVRIENLLAAG
                     KTVGVTHITNGCFRVHPVEWNIGEAAGALAAYCLHRRMVPRSVLAAPGDFQDMLFRQG
                     VQLAWPAELNLDDGEPHVHARPTRLL"
     gene            complement(17492..18301)
                     /locus_tag="VFQ79_15015"
     CDS             complement(17492..18301)
                     /locus_tag="VFQ79_15015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0141033.1"
                     /translation="MEIAEIAAIDVHGHYGLYCRKGLTIQNRIMSGGVEKVLERARTA
                     NIELTIVSPLSGLLPRMEADAAAGNEEAARLVEETPGLLQWVIVDPRRPATFGQAAER
                     LRHPKCAGIKIHPEEHGYPIAREGGAIFEFAARHRAIVLTHSGEENSLPKDCVGFADR
                     FPEVTLILAHLGCGWDRDLGHQVRAIQASEAGNIYVDTSSASSIYAGLIEWAVQEIGA
                     SRILFGTDTPLYSAAAQRRRIEAAEISDADKLAILRENAVRLLKPARHTSW"
     gene            complement(18327..20099)
                     /locus_tag="VFQ79_15020"
     CDS             complement(18327..20099)
                     /locus_tag="VFQ79_15020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0141034.1"
                     /translation="MRYDRLNAMRIPLVMHGPLVVLALWAGVAAASSPGPVELPFKGR
                     LGFTSRLADGRLFAVYLESRPALEWKDVSIPQHVVGRFSSDEGAGWSDPQVLFSPTGP
                     GILHAAYPLGTRDGNLHIFYLQKLDWDRPVDWSKTSALLMHTVSRDGGRSWQEPRSID
                     YGAQYTGALNSVVQLASGRLLVPLSYLVVPHRESGAFASTVVYSDDAGKTWKRSPSAV
                     TVDSGGKIVESGAIEPVVAELPDGRVWMVMRTQAGYMFESFSSDGGATWSPARKSVFR
                     ATNSPGAVLRLRNGSTVIAWNNEFDYNLFHGVSYCRQVMAMAIDDGAGWRGYRQVSAP
                     IQASEDIRSSVGYPFLLEAKDGGILLGYNERRVVGGRAGVRLFRVRPEWLRETASAED
                     FSAGVAGLYTVGTKGLEVTHGPEDRPAVRIQRTQAFEIGEPLPPSGMTWNFPFGRRGS
                     VNMKVRLDPGFGGADFTLSEYSIRPYHREGGVFRWSLGSDLRWRVQYANNGPYLPANP
                     PVINLLLSEPASRAETGRTLDLTVKWDAEQNVAWLYQDGRFVAFLLGLEAFRGISYLR
                     LTSAAADTDPAGFLLLGLRSRGEP"
     gene            complement(20102..23413)
                     /locus_tag="VFQ79_15025"
     CDS             complement(20102..23413)
                     /locus_tag="VFQ79_15025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141035.1"
                     /translation="MSSLKAAVAALFAGFAFFAIPAGMHAQVAGSALVGTVSDPAGGV
                     IQAAKVVATNVDTNLNRVTVTNEFGIYDLSGLVPGRYTVTVEKQGFRRQALEGVVLQV
                     AQKARLDVTMQVGQVSESVTVKAEAPLVDTETPEIGAVMTSEAVTQLPLNGRNFTQLA
                     SLTAGVNRSNDARPPNYNYNTAKQPSPTGAGAGSVGNSYTLDGTYNKDMWSGSYNVLP
                     PIDSIAEFKVEAALYSAAAGFGGATVVNAITKSGTNEFHGTLWEFVRNQDFDATNFFS
                     TVDASGRRQQPKLTRNQFGFTFGGPVLKDRTFFFASYEGTRERKGNTYTTRIPTAEER
                     RGDLSNYAAVIGKNIMDPTTGQPFPNNTIPVSRINSISATLIDAYFPEPTNPADFFRN
                     YVAAPSRSYDADNFSIRIDHRLSDAHSLFGRWSITKLDVFNPGANSSGLTRLGGQHST
                     NNWQNGALGLTSILTPSLTNDFRLNYNRSTDYLRGQNISDLLERTGLRYPLLRGDPAT
                     GVAMVSLSMSTTSMITSVLNLYRYDNANWNYPLRDDLTWVRGKHTLQMGAETQAVRAW
                     GLSDTTAGSVSFTGRFTGDGYSDFLLGNPFSMSISFPQSHRGDLSSRFFGGYVQDSWK
                     VTPKLTLNFGLRYELTSAPVEQSGHLVQWDPTLGNGVGGLRYPRQNRTAQEFYTNFRP
                     DLPFGFVDSDTIYDADKNNWGPRFGLAYRPFGGTKTVIRTGYALQYWVNLGLPQAAGW
                     AAVPPNKLSGSLTSSATTPTLTWESAYAGSFAGVTQQGVAFSVNAKQQRQNLNPYVSQ
                     WSVGIAHEILPNTVFKADYVGSKATKLWAAWDEQTPGPSSLPLADRIPFPKWGSITGY
                     HNAGTANYNGLLLAVERRFSGGLSFKANWTWSHALSRGGGWLDQGLLSRVQNPHDLKN
                     EVSRTGEDMRHVVSGYWVWDLPFGPGKRLFDIGGPLGHVIGGWQLTGLLYAHTGAPLL
                     WSVAQSFCNSNMGGNFCLPNVTGTAGQYTIGGQGVDRPKYDRAYFPSPNFTYGNAGMN
                     PLDMNSTWNIDAGLFKQFPITERLRLEFRAEAFNIFNHPYLIDLNARPDSANFALATR
                     AADPRDLQFALKLHF"
     gene            complement(23538..24896)
                     /locus_tag="VFQ79_15030"
     CDS             complement(23538..24896)
                     /locus_tag="VFQ79_15030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0141036.1"
                     /translation="MNLSIRLGALAAVLALSPSHGAGAEVITVPHAKVYQSPNCGSMI
                     SLKDGRLMWVWGVGGNHKPPYPMQVNYSSDGGRTWTDPQPVMTAGGAPVISFLSPSLA
                     RMKSGRLGMVQAHSQVNFNADNSQITSTFHYSDDDGRTWSKGVPVNALRRNDYPYFDQ
                     LSALSDGRLLIPAAHRVGPTPRDRSAHYFTLFGESFRNAYAPVLHTVTAYWSDDEGKT
                     WHHSENEVYATIDRGVGGIYGLLEYGVAELSDGRLLMLGFTGLGRIFRSYSRDRGRTW
                     EEAEPTDLQGSGPVSVKRIPGSNDVLIVWSQASRWETLSGYPRHRLSCAVTADGGLTF
                     RNFRNLESLDDVARIDPEPLKPYLQNRTWKQPTDTARYHRAPGPLRMDHPFTAFHDGN
                     AVVTYGVGTPGAPEMIRRFYGMTLDEFRKKFGLAADPRDPSRTQGNNRIQLIPIEELT
                     RP"
     gene            complement(24911..27691)
                     /locus_tag="VFQ79_15035"
     CDS             complement(24911..27691)
                     /locus_tag="VFQ79_15035"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU0141037.1"
                     /translation="MLLPMLGIILATSPGFQEIAFENELGPITRLADGRLFAATGQSR
                     GTRERWRDTGTPQRIFGRYSSDHGLSWSTPQLLFEPQGPGTLNSVRPLGTRDGRIHIF
                     FVQLLHLEQPYDWGKLSSTVWHAVSEDGGRSWREPAQIGLGRRWIPELQSAIQLQSGR
                     ILVPFAYANADSPVGRYAAAAVYSDNGGENWRESKNEVYVASGGRVGESGAIEPVAVE
                     LADGRVWMVVRAQTGYLHESHSEDGGVTWGVARRARFRSSNSPASVLRLENGPLLIAW
                     NNEFGYNLFHGVSYSRQAVALALQENGEWKGYRQVNPPFRADDDARGTATYSFLADPG
                     DGTVLLGYRERRRQGARPGIRLFKIHPDWLRVTGASENFTEGLKNLYLAGTGGVALRK
                     GPEEDPALLLSKTRKEEPCGFTWHFPLAAAGMVEMKLYLEPGFGGTHFSLSEYSLRPS
                     DAEGGTFRWMIGADLKWRIQYADNGPYMEVPGPHPNLTFGEIRSVAEAGRVLEVSVEW
                     DAGANTATLYQDGVFVAALVGMEEASGLAYLRIRSAADLDAKGIRVMELRSRPVEAEL
                     SGGFEGANPNRAGSILRQGPNRFLIRPFSEDPSNPDHKLNLLVAAGNPARRPLEVVVD
                     VEWDEPKEDQPHWMLYRSVLMSGRGDEWKAHQATSIEGSRARFTLSLPPGSSYLGLSP
                     KYTTSELREAIRRLPEGCAETITVGRSAGGRDIVGLRIARGDPQTRKAILLTSRMHPY
                     ETAGSFAAEGAIQWLCSPAGASLLDKYTFHVVPMANPDGVALGTSHLTAAGGADLVRI
                     GSDPSSAALKNLIDRIRPAGYLDYHQWMWRRDWIRTFMPEPETRARLFLNLEKTGFFD
                     RGWQLTDASGSAGREADLRHYAMRRWGTRVAVPSFTWSGKNPAQMRKAGELVLRAFCE
                     LFVPPSTTALQP"
     gene            27793..28944
                     /locus_tag="VFQ79_15040"
     CDS             27793..28944
                     /locus_tag="VFQ79_15040"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0141038.1"
                     /translation="MGSHELEVEASGVIFRNPFPGHRVINAFYPMIAPLDDGELLCVL
                     RRGGALYSPDGMLHLFRLGKGCDTWAHQGPVFDRIRDARQYNYSDGHLTRLGDGSLLL
                     RVARYDMTDPERFVINAATQGLASLQTCFLRSYDAGRTWSEPVVADVKEHFAPGVEPA
                     PLGGVVELPDGSWFQLFDTWKDYENGGLFDVNGYGLFSADGGRTWRDKVAVAVGTAEE
                     RSYSHGIAAPMRDGRVFVSYWTATQDLQTSFDLHTVISTDASARQWSRPLPAGVPGQT
                     SCVADAGNGCMLMIYSHREHTEQPGIKVVLSTDAGRTWRRDEPLVVWDAYGKESLGVA
                     RSNTYPSSHDAIAYGAPRVVALDGGSAIASFWCSQGGDTHCRWCRIRIR"
     gene            complement(28941..30197)
                     /locus_tag="VFQ79_15045"
     CDS             complement(28941..30197)
                     /locus_tag="VFQ79_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141039.1"
                     /translation="MNVASGVQEDTAPPEADRVNSRIYALWACLVYLAAPVTYVGVVQ
                     AALCDKLGASPTVANLPASLYLLGFAAPLVVAWAVPQRYERMAAAAANGLSCVMLTLV
                     AAVLWLPAGNSLRIAAIIGQGCTMGVTASTAQVFMLQCLCRGTNEAGRARGFRWAFGA
                     GPIAAVAGSLGAQYVLEGGIPALAYPRDFALLYSFGAVCLAVAAAGALRFKLPPAEEP
                     PRLPMLKHVAASVRSFAADRPLRRLWMAYVLWFTAVYAAPNFSLYTRELLGRDPKELS
                     GLIMALRFASKSAAGFLLASLILRRGARAPVVAAVLLTVAAAAWAGMVPGYLFLLAFA
                     LLGAGELGGAYFPNYVAGVSLLAAGARNQAVLALATPAASFGPALHGLLTVRYGFGAS
                     FVFAAVAALGALMLVCTLPRGAGSKP"
     gene            complement(30194..31159)
                     /locus_tag="VFQ79_15050"
     CDS             complement(30194..31159)
                     /locus_tag="VFQ79_15050"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0141040.1"
                     /translation="MNRTIVAPLDSGAESRIERLRGLWAALPVSWTEEDELDEEAYRE
                     NVARICRAGLHGVYTHGTTGEFYAQREEEWRRVARATVEESRAAGVPCQVGCTALWTG
                     EVIRRVEFAGQLGAFAVQIAFPFWMALSDSQAVRFFREVTCAVPEMPIVIYNTERSKK
                     PLTTDLLGRIMDAGVPLVGLKGAGDAAEFAALSSAAPGVKIFVSEHLLAPLWKEGARG
                     SYSAFVLACPGFMLRYYRLAEQESPEAMRIAAALARFLAEFVAPCVARGLYDTALDRC
                     IAATTGFLGGRILASRAPYDAAGECDVKLCREWLCRNLPEFLEEV"
     gene            complement(31152..31556)
                     /locus_tag="VFQ79_15055"
     CDS             complement(31152..31556)
                     /locus_tag="VFQ79_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0141041.1"
                     /translation="MYDRGVRGAGRGRQGGWTAAGRLLVAAALVCGSLRAASSDLKAT
                     VNAHMARLGFSRTHIAGNVTAMHYRMAGIEPAIATLEARIRSGNLMTAGFADRSPRGT
                     VTTAREAGRLLERLCKGEIVSRVRSGLGGTHE"
     gene            31616..32782
                     /locus_tag="VFQ79_15060"
     CDS             31616..32782
                     /locus_tag="VFQ79_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU0141042.1"
                     /translation="MDSRILAFSVAEYRDRTRRVQDRMAELGLDALLCHSFDSTCYLT
                     GLQSVMGPAKYFMTVVPAEGDPFLLGQDFELHNARFTSWVEDLTAHPVFEDPVEASRV
                     LLAGRGLAGKRLGVEMKRLCMNPLQFASLRQALPEAQLVDASSLVPSIRMLKSAAELD
                     YMRRACRISSRAMEAAVGAVAEGRTDNEVAAVAVEVCFREGGEFFCLEPIVSLGDRSS
                     VPHSSFAGNRARSGDLCFIEIGANIQRYSGPLIRTVALGEPDAEVSRAGAEAAASLNA
                     VIAAMRPGVTAQAVAFEGRKAWEWTASHPEYVWNGAYAYSVGLGFPSDWCWCDAWLFI
                     HEREEAVLQPGMVFHCTNNIRRPGVFGLGISETVAITDSGAEVLTSFDRRLLVR"
     gene            32858..33493
                     /locus_tag="VFQ79_15065"
     CDS             32858..33493
                     /locus_tag="VFQ79_15065"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0141043.1"
                     /translation="MARASLPEVRFVSMRASVADTLRQAFLDGYFSPGEDISEVALAT
                     QLKVSRGPIRDALLILTQEGLVTHSPNRGFSVLQLTDSDFEHIQNVRLPLEALALTLA
                     RGRVSREDVEILETLKEKLMEAFRAGNFSAAARCDLEFHSALWERSGNPWLEAALKRI
                     MVPYFTFTMVYRQKPSTLTEAIIEEQHALYIDYIKGESDKSAEECMRIHLG"
     gene            complement(33481..34746)
                     /locus_tag="VFQ79_15070"
     CDS             complement(33481..34746)
                     /locus_tag="VFQ79_15070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0141044.1"
                     /translation="MDRNGARKWWVVALLFWAAALNYGDRTAITAVFPLLQSDLGMSD
                     VALAATGSFFLWSYALASPLAGYLGDRFSRTFLIAGSLFAWSAVTLLTSFAANSPQLL
                     GMRVALGVVEAAYMPAAVALLAAWHGPATRATAMAIHTAGYSAGMVGGGALAGLLGER
                     MGWRSPLVVLGALGLLLSLAASRFFKRLPAHSGGETCAGENASAPPLRAIAIVFGNPT
                     CRLILLQAMLASTAMWVLLTWMPLYFLETFGMGLAEAGFAGTFYSQTGIIVGLLAGGR
                     PSDAVGLKGTEYRILLHCGLYLAAAPLLLVFLWSRELYVIAAASVCFFLIRSLGICNE
                     HPVLTHLLPAGLRGTGIGLSNSLNCLAGGAGVLLAGHARRAWGLPAIFASVAALYVLC
                     SAVLLLAYLSYVRRERWQRPTPVRTLQPR"
     gene            complement(34765..35553)
                     /locus_tag="VFQ79_15075"
     CDS             complement(34765..35553)
                     /locus_tag="VFQ79_15075"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0141045.1"
                     /translation="MCSSRVRMRLRRKQPVACTKINTHDPAVVDLIGLTGFDCVWMCC
                     EHVPLDADRLALLIRAASQHRMDSLVRVARFGYSSYIHPLELGATGIMVPHCMSAADA
                     REVVKMTRFHPLGRRALDGGNADGRYCTVSVPEYLQHANDNTFVVVQIEDPEAAEQVD
                     EIAAVDGVDALFVGPGDLAHALGIPGQIEAPAVQKVIECVALACEKRGRAWGLPVNAE
                     NVERYLEMGALFCNVGADVLGLQVYYSEIRARFSKLGFEFDRMV"
     gene            complement(35614..35973)
                     /locus_tag="VFQ79_15080"
     CDS             complement(35614..35973)
                     /locus_tag="VFQ79_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141046.1"
                     /translation="MYSKSGRRDYVKSHLVRRRLEHYSHIQTKAKREVLEVAGVNTYT
                     RDDYLFTPARATSKTSAATGLTSDDARLAPGDVSFVLMRATDDVIVTWTNGTSRKEQS
                     FLVNIKLLVVDSCFFSA"
     gene            36020..39409
                     /locus_tag="VFQ79_15085"
     CDS             36020..39409
                     /locus_tag="VFQ79_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141047.1"
                     /translation="MKASRHLPLIVFALGVSSAPCFSQVLYGSLVGNVMDESQAAVAA
                     AEVTITNQETNLTRRAVTNEVGLYTFPSVPSGQYTVKVATPGFGEFVRRDVPVAVNTT
                     VRVDALLRVSGVAEQVTVTAQPDAAMLQTDRAEVRHEVSRRQIENLPVPVGRSYESLL
                     QLIPGFEGGSAREERLGGNRPERSQNFNVSGTTNSTVARNLDGASNMHVWSVGGSAFV
                     PNLESIETVDVVTNSFDAEQGQAGGAVINVQIKSGSNQFHGSAFEYHYNQHLKARDYF
                     RPAGEAKGKQISNQFGGTLGGPIKRDKVFFFTSWEGRAERENLGRLLSLPSRALRAGD
                     FSEFSAVIYDPMTGNANGTGRLPFEGNRIPEHRMDTIARKVISLVPLGNLPGMQDSNS
                     NNFFASVPFKANSYRTDNKVNWQARDNFNLFGSLSYFNFDVINPTAYGESIDGPRVSG
                     GNAGHSWGYNRRISTGANYIFGPTLLMDAFFGWTRQNTNVEQPGIETNYGSDVLGIPG
                     TNGPNRFQGGWPMFSVSGFAVFGTEEAYSPYYRNDDQFSTRSNFTKTLRSHEIRWGVD
                     INAEQMNHTQPEFQGGANTGPRGRFNFDSGPTQLCMAVAANGNCQTLSTAVNQANAFA
                     SFLLGLPTQLGKNLLTEAPYNTRTWRYSLYARDRWQTTPKLTISYGVRWEYFPMPTRT
                     DRGFERYNPEDNKMYIGGVGNVPKDLGVHSSKTLFAPRLGVAYRATDTFVVRGGYGIN
                     FDPYSLAKALRTNHPMLIELVVPSRNSLSFAGRLSDGIPPINAPNLGNGIIDVPGNVS
                     TQTIPLDLQRGYIQSWNLTLQKRLGWGFVGEAGYVATRQVRQLGYRELNWSPVGGGTA
                     GQQLNRRFDRTANTREVGPIGGSHYDSLQAQLQRRFTGGALLQVAYTLSKSISNSGVD
                     SSAGAPPIIIPDFYHLNRSVSSFDRRHNLQVANVTPLPFGRGKHWLNQGGILSAIAGG
                     WQANSIVSVMSGRPFSVLASASSLNSPFNSQRADLMKTDVEILGGVGRGQPYFDPTAF
                     AAVNQARFGTAGFNILRAPGRVNWDLGLFRNIRITEAVKMELRLESFNFTNTPKFGAP
                     GNNVSNLQFNPDGSIRNLNGFSEITSAFEERQFSLGVRLSF"
     gene            39422..40687
                     /locus_tag="VFQ79_15090"
     CDS             39422..40687
                     /locus_tag="VFQ79_15090"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HEU0141048.1"
                     /translation="MNKVRMCIAASVAAITLAVATEVAVPPASSRELARRKHHVANSP
                     RTPAPEPGQENTTILSPLLKPNGEKVTSAAEWYSTRRPELLKLWTEVLGKTEPNEADR
                     KWFGDITKTIIKSTEEQPGYTRIDLDIPLETDFLQHHLLLIPRDQGRGPFPAVIAWAA
                     SSGDYRDPERWWGEFLASNGYVVLSSWSLMRNYRNATTSRTGAPDLLYDRFGRWLPIA
                     RMAYDIRRQAQFLKTRPEVDGSRIGFIGFSHSAKAAVYAGAFAPEIQAVVSVDPHIAI
                     NGGTNYYDPWYLSWQRKFKDIRTETGTVQSLLNPDIKRPGFERDHHELLAMAAPRPFL
                     LIGGDQSEGDGGHSDDLQSWGYFNHAKEVYELLGIAERLQFASTADGHKANGPNIDPA
                     WKTFFQRWLKETPVRFTGWRDQYGPARNR"
     gene            complement(40780..42405)
                     /locus_tag="VFQ79_15095"
     CDS             complement(40780..42405)
                     /locus_tag="VFQ79_15095"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0141049.1"
                     /translation="MRPTRVLLIALFVMAICYAVLATGDASISVRIVDARTGKPTPVR
                     VRLLDSNGNPPKITGALAVSDAAFGVPAAAIGVMYGQSDRAEGFLLQPDGAFYVDGSF
                     DVKLPAGNYTLSLSKGYEYIQQTHKLSISAGKRTRTTYRMEHWINMPERGWYSSDDHI
                     HLRRSSRDDASIVRWISAEDIHVGHLLQMGDFWATYYSQYAWGEKGRYRERDNILSPG
                     QEEPRTPEIGHTISLGAEDFVRFQPDYYSYDRVFDRVHELDGITGFAHQAMTFHGYRG
                     MALNVLRGKIDFLELMQFCAAEGPLVVDHYYRFLDLGYRLTALAGSDFPWCGRGPRYG
                     LKEGGSQIGDSRFYTYVGNEFTFASWLKAVKAGHTFASSGPIVQLTVNGRLPGDTVNV
                     RRGEVLKIKATAFGESKQIPLEELEIVGHGEVLRAARAAQPGQSAAQLSIDMEMAADH
                     GLWIAARVKAGPTQLAHTTPVYVSVDGGGFENRGKFRQRISECEQHLDELEKELVSPG
                     ERLDHQATRHKKSLLRQISETREVLASLKSRSE"
     gene            complement(42433..42509)
                     /locus_tag="VFQ79_15100"
     tRNA            complement(42433..42509)
                     /locus_tag="VFQ79_15100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42473..42475),aa:Val,seq:cac)
     gene            42583..42658
                     /locus_tag="VFQ79_15105"
     tRNA            42583..42658
                     /locus_tag="VFQ79_15105"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42616..42618,aa:His,seq:gtg)
     gene            complement(42669..43982)
                     /locus_tag="VFQ79_15110"
     CDS             complement(42669..43982)
                     /locus_tag="VFQ79_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0141050.1"
                     /translation="MRAITALFVFFSAAVAAPPLFEQTGFQTSADGSPAGWKSWSPRA
                     EIAPRTFVDAVHFRKRPGSLAVSGNSRVGVFGGWEYDAKGIEPGKWYRLVAHYRSEGL
                     DYEPLQVWARLDWRSSAGRRTGQPDYGYRVTRDGEWNRITLEAPAPEKASGAAIQLFL
                     LNAPNATLYWDDVSLTQIEKPGPRNVRIASINHRPKQQPSSAAAVHSFLDYVEKAVGE
                     ADLIVLPEGITVVGTGLKYADVAEPLPGPTTKALGELARRKNSYIVAGLYEREDTTIY
                     NTAVLIDRQGQLAGKYRKVYLPREETEGGITPGSDYAVFQTDFGTLGMMICWDVQYAD
                     PARALALRGAEMILLPIWGGNETLAKARAIENQVYLIASGYDHPTYIMDPDGEMLSKA
                     STLPGVASATIDLNRRYQDRWLGDMKARLWKELRLDTAVDPPGRK"
     gene            complement(43987..44481)
                     /locus_tag="VFQ79_15115"
     CDS             complement(43987..44481)
                     /locus_tag="VFQ79_15115"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU0141051.1"
                     /translation="MTDHHILDSLESHPFTQGLTADQLEVLAGLAEPVSFEAEQTVFR
                     GGERSDYLYLLLSGTAFLELQTPVYAVCIQVLGGGEAFGWSSVVEQRYAAFQVRARQR
                     CEALRLNGPLLLEACEKDPAIGSTIFRRVAELIATRLRATETRFIEFCGGVPGARPSA
                     IGTQ"
     gene            complement(44499..45773)
                     /locus_tag="VFQ79_15120"
     CDS             complement(44499..45773)
                     /locus_tag="VFQ79_15120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141052.1"
                     /translation="MLYFVALLAGFLQAPSAQAPALVEAGIELSHQGRFNEAAEKFVR
                     ALAMDPKLAEAHYLLGLVRQTQGRAAAALQSFRSALDVNPRYAEAQARVCELLTVFAR
                     AKDSGYDEARDSCRAASALDASDPEPHFHAGWNEAKLGNLAAAVQEYETVLRLNPKFP
                     KVKFELAMAYLDTQETGRAIILLRDVLAVEPGNGAASFQLGAALAKKDDCEGAVPLLE
                     AGAEVPQKYYVLAGCLKKMGREAEAAAAMARVKDLRTGADARMQAKYRAAVAHQKAQA
                     GDLDGAIKEYRAALALVRDSSIAVDLAVALLKKGEAEEVVRLLGGEPDSLARYQLALA
                     YSKLRRFQDARAILEIVLQSRPEFVEAWYQLGINSLAAGDPREAENSLRRAVELRPDE
                     PAIRLAWADSLEKLGKRKEAGEQRTLAGRFPK"
     gene            complement(45798..>46332)
                     /locus_tag="VFQ79_15125"
     CDS             complement(45798..>46332)
                     /locus_tag="VFQ79_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141053.1"
                     /translation="VCERAHRRVADCRSGVCYDGWLYWNGYIPANRINSVDSAGRPNG
                     VMGVPENYRPFQTWLIPTPANGGSSSDPNFAFYESNTVFVPLKNGTNQRTTYDTGMHR
                     LQNQYFTAPIRWNMAASAFKTVPITERVFLRLNADFLENVFNMPGTTVPGGDGIVLNR
                     TSDNAPRVLQLTIRLTW"
BASE COUNT         9083 a        14402 c        13994 g         8853 t
ORIGIN      
        1 ccagtacagc atctaccttt tcccgtggga agtatttcgc tatgacaccc agactgatgt
       61 agtcggttat acgactgcct gcgggcaacg atgcgacggt acgagccatt ctgggggcct
      121 cccgccctaa acagaatagc ataaaaatgt tctaagtgaa cagtattgca cctagcacga
      181 tagtcggcgg gcaactgccg gctggcctcg cgatcacaca tcgacgctga tgacaccgcc
      241 gcggactacc gttgatgcgg cgatcttgca agtcggttcc ccgcgggagt tcgtccctct
      301 ggctgtcttc tgcaaggcca ggcgtcggct gaagagccga cgcggcacgc tgaagcgtgc
      361 gccacgatgc cctgaatgtt tcctcggcga ggccggcgtg tgatcgctga aacgtccact
      421 ctgcaaaaga gctgtttcta ctgagaaacc accgtgtcgg tcctgtcgcc ctcggccgtt
      481 tgcacgacga ggcgagcttc actcggtgcc gattccagca gtattttccc gagccggcca
      541 gcgccgaacc aggcgcccgc aacagtatcg ttcacgcgga ggatccacgc gtccccagac
      601 ttttcggcgc tgatctgagg ctgcggttcc gatgcgcgat agggaaccat caccgccagg
      661 aatttggcct ccgccgcggg ttgggtggtg cgcgccgaaa ggtgccactg attcgggaat
      721 cgggccgcac aatctttggg cgatgccgcg catccggaac tctggctcac ctctggcgca
      781 actgagaacc ggtcggtctg cgagaaccgg aacggtactg tgctgaccaa acgcacagcg
      841 aggcgggcat ccccgtcgcg aacgtaaatg gtgcccgtct gactgtcggt ttccatctga
      901 ttcagcgcgt gtgccagcca gccgaacgtg gcaggcttcg ccgtcaccag atagtcgtgg
      961 acgatcagca ccgggcgctc tttcgacccg acaaaagcga gcgtgcgctc gaacgtcttg
     1021 gcctccggat cgagcggcgg cacaggctct ttcatgtgct tttgccacat cttcagcatc
     1081 cctggcgtct gctgcaggtt gtatgcggag gctgcctggc cccgggcgag cgtaatcagg
     1141 cctttccgct catagtgctc aatgctgcca ctcgcctccc atcgatgcgt gggctgcccc
     1201 cgaccgttga tcaggatgcc gttgtgggca cgcgtttgcc gggtccagag accgtggtgg
     1261 gcacttccat accacgggta gtaaccggaa tcgatcagca gcgcgcggcc gtaagcgttc
     1321 agttgaaacg tgttctggtc tgcatggctg tgactgaagg acccaaagcg gctcgacttg
     1381 aacagcgccc acacgtcgtt cgcagcgtct cccagagccg agtgcatagc cacccagcca
     1441 atatcggaga ggtaccgcga gccgtccagt tccgatggcg gctgggcctt gcccggggcg
     1501 ccgtttgccc gcgccctcag cactgagccc acgtcttcga tggcccactc atgccatttg
     1561 ttctcggcga tcgacggcgg tggaaccggt atcgcctggg cattccagcg gagcacggga
     1621 tcatcgaacg catccgcgaa gcgctcgagc atcacctgct ggctctctcc cggtccgcgc
     1681 tctgctccgt cgccgaacgc tccacgcgga gcgtaggcgg gctggaaata gacggcaaaa
     1741 tacccggtgt tctggtagaa cggacgccgg aacaggtcta cgtcggtgac gccgcgcaaa
     1801 gcctccgcga atgtcgtcag ataatacacg taggacgacc aatagctgac accctgtgcc
     1861 catccccctt gatctccgcc ccagccggga taagacgtga gatagcagcg caggacgtaa
     1921 tccagccatt gttcggcttc gggaatatca cccagaaaag ccagcccggc gttgccgagg
     1981 aaggccagca cgcgaccctc gtgattcgag aacggatgcg agaggaagtc ctgagtgcgc
     2041 agtaacgcca gcacctggtt gccccgctcg gtcatggatg cgagcacctt cttgcgctcc
     2101 tccggcgtga gcttgtcgcc gatccagtcc caggcgaacg acatgcgatc cagcatgggc
     2161 atagaggctt cgtcgttgcc gctcgacccg ttgggctggg gcacatcgtg gctggtggag
     2221 ccgcgcgggt cccaactggc caaatgcaag agccaccgcc gcgcgccttc cagatacttt
     2281 tcttctcccg tgatccggta agcgagggcg aggcgcgcca cgtgggcact cccgatctta
     2341 cccggcgtgt agatccggcg ccagtcgtcc acgttgaact cagcagtcgg atagcccgcc
     2401 ggttcgggat agagcgtctc ttcgagcgcc gcgtcagagg cttgcagcat cggcttccac
     2461 caccggacgt tccctgaggc aacggcagcc ttgatttcgg ctgtgcggtt agctgtcaaa
     2521 aagagccttg gccgaacact cgcaagcttt gttttgaggg ccgccacatc cggcatcggc
     2581 aggagcggca ggctctccga gagcttgaat gtgcgcgatc tcgatgcgcc ggtgggtgcg
     2641 ccgttcgcgt ccagataaac cgcctgccac acgtagtcgc cgggagcgag cggtttactg
     2701 gaggcgtaca ccgtggaggt ctgtggctcg gtcgcgatca ccgcgcggga agccacatca
     2761 gcgcggcgaa cctcgttttg gccttctcgt tcggcgtcca ggtgaaaccc ggcgggttca
     2821 cgccggcgct ctggccatcg gccggatatg caatcaaatc ctgtgggtta ggttgccact
     2881 ccacgacggc tatccgatca gtccaggggc ggctctgctc cccacaccct gcgagcaccg
     2941 ccagcgcgaa aagtatcgcg aaaatcgggt tgtgcatctc ttctcctatt gaatgctcat
     3001 caaataaatt ttgggctccc gagcgctgca acgtaaagca cccacaaaaa ctaagggccc
     3061 tccagggacc tcttccagtc gagtagatgc ctggtgcact ttcgtcagaa ctcgatgcga
     3121 agcgcgacca taccgttgcg tggaccgccg ccccccctcg cgcgcaaact tcggatctgc
     3181 cccacctcgc cgggtacgct tatgttattg gagggaatcc cccagccagg atggttgagc
     3241 gcattgtcga aggtgccttg cagaaccgca gtgacacgct ccgagatgaa gaaccgctta
     3301 aacagaccgg cgttgaggtg gaaaaggcca tgaccctgca agacgttcgg tgaagaattg
     3361 ccgaatcgcc ccaccgctac atccggtcta tggtccccgt ccaggtctcc aggaacgcgg
     3421 aaggccgcag ggtcgaacca catgctaaca gaccgctccg cttttggaag gtttccgtcg
     3481 gcgattcggt ctggccggcc tccgatcgta ttcgtgttag aaacgtcgta cccgctaaac
     3541 actggattaa agtaaagccc ggtatccgtc atgagcattg cggtcaggct ccacccgccc
     3601 agaatgaact tcggcacacc gcggatcgcg ctcagccacg gcttccctgg tccgaatggg
     3661 agttcatagt tcaggtttgt caccgtgcgg tgccggggga tgtaatccgc gttcgcgcgt
     3721 tccattcgac gcgcgtagct gttttccggt gtgcttccag gctcgccgtc cgacagcaca
     3781 tccgtgagcg ccttggccca cgtccagcca aactgataat agagaccgtt tttcatttta
     3841 cgttcggcca ctacgtggag tgaatggtaa tcatgatttc cgccgttccg acgcagggca
     3901 actgtcctga aggccgggaa ctgtctcata tcgttcctga acggctccat cccgggagcc
     3961 ggctggttca ggttcgccgt ccagatcaga tgtcgcgagt ttgtgccaat gtaggacacc
     4021 cggacgccca tatcgaacag ctctcgctcg acggtgacat tccactgctg tatcacagga
     4081 ctccgcaggt gcggatcatt cgcagtgaag ttctggacac cagttgcacc gaatccgtcc
     4141 gggaaggcga gcgggaacat gaaattgggc ttccctccga cgatcgcgtt tgtgaaagtc
     4201 tcgtccgaga tgtagggtcc gcctactatg ttgttccacg cagcatcggt gatctgatcg
     4261 gtgtacatac cgtagccgcc acggattact gtgcggctgt cggcgaacgg gcgaaaagca
     4321 aatccaaagc gcggcatgaa gtgtttcttg taggaataga aaagattgcc aggaaggccg
     4381 gcgtcccgtg cggtaacgat gggcgcgagg ttcgctggga acagcggatt tacgtgtcgc
     4441 tgtgactcct cgctgggtac aaccacacgg ccgctcgtcg ggtcaaaatg gaatgacaat
     4501 ccatttcgct cgtgatacgg cgggttgaag tcccatcgca atccgatatt caacgtgagc
     4561 cgccgtgtca ccttccaatc gtcttggata aatccggcga cgtcagtatt gctgagatgg
     4621 tacatctgtt cggccgggtt agtacgattc gacgtctggg ggatcccgag caggaagtct
     4681 gcgtacggga aattgctgta gacgcccgtg aaccctgaat gttccgaagg ctcgttgtac
     4741 aaacccgcct cctccgacga agttcgcgcg tgtgttatgg cgaatatcga aacccgcctt
     4801 aacggtatgc acgccgcgga tccacgttac gttatccgag aactgaacgt gccgttcacg
     4861 gtcatcactg accattgttg cagccccaag gctcgcgaac ccggttacgt tgaatctcgg
     4921 tgcgccggtc tggatcgtta catcaggcga gagaccttcg agcccgaatt cccggataag
     4981 gtcggccccg ttcagcggat tgaaatatgg atactgtatg cggagcgttc caaaccgaaa
     5041 ctcgttgatt aaagacggcg tgaagatatg cgtgtcggat atagttacta ccgtcgtact
     5101 gcgaacgcct cgcgtatatg gaaaagtcgg gacggcctcg gactgcgact cccatccgcc
     5161 gcggttccac atcaccctcc cgaacatcga gttctcattg gtcagcttat gatcaattct
     5221 cgtctcaacc tgagtcttga accacttact cggcagagta tcgcggaagt tgctttgata
     5281 gctgccaggg gttccggagt tgggcagtgg atagaagcgc tcctggactt tcagcgacac
     5341 tgggttgagc cggtcctgcg gaatcacatt atccgcgaat ggctggccat tgagtgggtc
     5401 ccggatgacc ctgccgggca gaagcgcgga gaaatctccc tcacgcatcc tcatcgtcgg
     5461 cacactcgta ttcagaacgg tttccatccg catcgggaag cgctcgtaca tcgagtgaaa
     5521 gaaggtacga ttctttataa tcggcccgcc gacggcggcg ccgtaatcat cgtcatctct
     5581 gaacggcaca gtcaacgaga atggtgagcg cgctctcaac cgactggagc cgagataata
     5641 aaacacggct ccgtgcaggt cattgccgcc cgatttgctg ctcgcgtaca tgccgcctgg
     5701 cctgagtgcc tcggccttgt cattggcgag ttggacacgc atttccgcag tgctatccat
     5761 tgccccctgc gcggaaaagc tttctatttt tgtcccgtca accgtcgtac cgtactgtct
     5821 gccgcgagca ccggccaaac tgaaatttga gccttgggcg cgttgagcgc ccggcgtgag
     5881 atgaagcacc gaggagtaat acccgctcgt cgagtcacca cgatagttga ccggcgcctc
     5941 gagtataatc ttcgcgctgc ggacgttcgc gacttcggcg ccttccgtat tgataagcgg
     6001 ggcagacgcc tccacagtca cctgcgttgc caattcgccc accgcgagca ccgcgtcggt
     6061 ccggaccgtg gctgaggttt ccaggatcac atccgagatc acgtgtgtgc gaaagcccgt
     6121 gcgagacaca ctgatcgagt atcgaccggg gggcaggttt gtcacttggt acaggccgtt
     6181 cgctcccgta gtggcatgat gttcgatgcc cgtcgccata ttccgtacga caacggcaac
     6241 atcaggcaac agggctccac tcgaatcagt cgtttcaccg agaatcgttc caagcgtcgt
     6301 ctgggaaagt gccgcaattg tgacaaagga aaacaggaaa attaaccgaa acactcgcat
     6361 ggcgggatct cctctctccg cttcctctac aggcaggatt aacggatccc aatcttcttt
     6421 tatgggacga agtttatatc aggtattggc ccgtgtcaat ggcgagttga catagtgcga
     6481 gttgacatag tgtcccgtat tgatgagttc ggcttctatc tgctggagag tcggcaggct
     6541 ggcgatcgcg aaaactctgc agagggtccg ggcttatgga agcagttcga gacgagtcca
     6601 gcgaatcagg cataccccgt cctcgacaca ccagtgtgcc gcgagcagca ggcgggcatc
     6661 caagcggatg acgcacggta gaccgaacgc aaacgtagac agctcctcta ggcctgacgt
     6721 ggacagtgtg acgctgcggc tagcccgcgc gtcccacaac atctcctccc actctaccgt
     6781 ccacaactcg gccgtgtaac gcaccaggca catgcgcact ccctgccttc cgtaccggca
     6841 gttgtagagg gctgcgatgc gatcaccgcc cagccagatc ggcgtcaatg tctggccctg
     6901 aatacctgtt ggtcgcgctg gactccaggt gcggcccgca tcggttgaca cgctgaagtg
     6961 attgtccaga tcccggtagt cacccatgcg caccgtccag gcggtcgcga gcagccggcc
     7021 cggttcggtc tcgatgatct tctgttcaaa gaagccccgc acgccctcgg gatcgtggaa
     7081 cacgattgtc gaatccggcc aggacacgcc tccatcgtcc gagcagaaca ctagggcggt
     7141 ctcccctaac cggtcgggat gaaacagact cgcggccgga gcgagccagc gattgcccga
     7201 aagcacgacc atcggattcg agacctccca ggcatcgccg gccgatgtag ggatggtctg
     7261 cacaggcccc cagacgcgac caccgtcgag cgacagggtg tacaccggat cgcgacgcag
     7321 gcgatgaccg aacgagcggt ccccattctg tgagaacttg cggaatccgt aagcaatcag
     7381 cgtcccttgc ggcgtgcggc tgagccgcag cgagttcgtc gtgggcgggc tgtcagatgg
     7441 ggacagaatc atcgactcgg tctgccagtt gcgcccgccg tcacacgatc gtgccagcgc
     7501 ggttccaccc agggcctgcg ggccgccacc gacggtgaat gccgccagga tttcgccgtc
     7561 gtcaagcctg atcaggctcg ggtaagcggc gtcgtccgcg ttgatgatgc cggtctcgac
     7621 aatccgcaga ctacccacgc acggtctccc gttgctcgac gcttttcact gtgttccgcg
     7681 gctcgatcag gtgaaacagc agtcccgcaa cataggcggt aagggccgag aatgcgccgt
     7741 accacatcca cgagacggcc gtcgacgccg agagcacggc gacagcggcc gaccccacca
     7801 gcaggccgaa gaaggcagcg ttgccgccga cgtggcgcgc cgtcatgccc agcagaaaga
     7861 tccccaccag gcttccaccg aaaaaactcg tgagcttcgc agatgccaca atcaggttcc
     7921 cgagcctgtc ggcaaacagc gcgaacaacg tgcagcagat gcccagcccg aacgtaaccc
     7981 ggcgcgcgct cgcgaccgag gaagtccgct gcaacagcgt ttgtccgaaa tcgaccatga
     8041 cgactgtagt gagagagttc aggaccccgc tgatcgtcga catagcagcc gcgagcacgg
     8101 aggccacaag caatcctcga aatcccgtcg gcagttcctc ggctatgaac ttcggaaaca
     8161 cgcggtccgg cgccaatccg atgccaccct tatcggcaaa ttccaggtag tagacataca
     8221 gccccgtgcc gacgagaaac aacagcgcac cgaggcatga gctgatcacc atcgacgacc
     8281 cgacggccca ctggcctcgc cgcatagatg aggtggtaag gaatcgctgg acggacgctt
     8341 ggtccgtccc gtattcactg accatcagga agatgccgcc gattaggccg ccaaggaacg
     8401 taaattccgt tgtcaggctg ggactgaagt cgaagaagcg aagcttctgt ctctccccgg
     8461 cgacggcgaa cacttcgctc cagccgccgt ccagacgaat cccgattacc aacgccgccg
     8521 cagtcaggcc acccagcagc acgaacaact ggaacgtgtc tgtccagatg accgcacgca
     8581 tgccgcccaa ggtcgtatac aacgcggtaa gcacgccggt cacaacgatg acgaacggca
     8641 gcgagaaacc agtgatctct gccacgaaca ccgacggcgc atagatcacc acgcccatgt
     8701 agcagcactt gagaaccaga aatatcgcgc tgcccagcag ccgggttttc agatcaaagc
     8761 gccgttcgag atactcgtaa acggtgaata cctgcatgcg gtagaagtat ggaatcatca
     8821 ggccgcagac cagcaggatg gcgaatggga gagcgagccg gtactgcagg tatgttaaat
     8881 cagccttata ggcttggccg ggcgcaccga ggtaggcgac ggcgctgagc accgttgcga
     8941 cgccggacag cgatgcggcc caccaaggga tgttgcggtt cccaaggaag aactcctgca
     9001 acgttttctg acccgccgag aagcgagttc caatcagtac gacggtgcag aagtagatcg
     9061 tgaggacaat gtggtcgagg agcgtcattg tgagtcgtcg cccggctacg ggagcactcg
     9121 gaaactatag agttgagctt ctttcaatgt gaagcggagc cggactggct tcgcggtgga
     9181 aagcggggtg ttcgatttcc agcgcaccgg atgcgctact cgatctccgg ttatcagggc
     9241 tgcttcatcg agccttttgc ccgtgtcgtc gagcaattcg acgcgtattg atccgggcgc
     9301 ggcggccgca ttcacctcca ggcggtgacc atcgaattta aggagcttcg tcgtcacggt
     9361 cccaccggcg gcggccgcat ccatcgagac gaagccgtcc atgcgccatg tcgcgcggcc
     9421 gatggttgct actttgtccc gcatcggagc accatgggtt gccgtgtacc cagtgtagta
     9481 gaggtggacc tcgtcgcctt gagacaccag cgtgctgccg acgcccagta gcatgccgcc
     9541 gtcgaaggtt ccgggtttgc cgattgcgat aatgggcgat cggtcctcga accgcttcca
     9601 ggtgtagccg tcccggctgt acgtgagttg cacgtcgatg gggccgtcat gcggactttg
     9661 gtccggcccc acctcgtccc gcttccacac cttctccatg cggaatgccg tgatgaagcc
     9721 aaggaagtgg ctgccgtatg gaatgccgga catgttgtac cactccatgc gctgctccgg
     9781 cgttttcgcc caggcatcgt cctgttcatc gggcggcaaa atcagctttg gctccgacca
     9841 cgtctgaaag tccttgctgg ccgagagcca gaccagccgc cggtccaacc cgcgcacctt
     9901 tcctggtcgc ttgtgaaatg caaggaactc acctgtacgc ggatgctgaa ccatcgagat
     9961 cgtatccgag tgtggaagca ccgggttgat tggataggcg tgccagcgta aaccgtcggc
    10021 cgagtatgcg gcccaatatc catgtgcgct ggacggcagg ccgcgatgtc gggccacaga
    10081 cacgcctatc atcttgtatc gtttgttcgg atcggactcc cggcggtcga tcaccacact
    10141 gggactgtgt atgtcgtaga ctatgttgtt gtgcttcgag ccggcaaact cgatgaggcc
    10201 gagcgccggc ttgtcccagt ggatcccgtc cttcgaagtc gcatagcaga caggatcgcg
    10261 taacgccttc atctccggat aggccgactt atgcagtgcg ccgttgtacc acatgcgaaa
    10321 cagcttgctt tccggatcgt ataacactgt gccgaaaaga ttgacccgct taccttccca
    10381 cggcttgtcc ggttccacca ccggcctgtc cagttttcgc gccgggtgca gagtgcgccg
    10441 cacgcccgcg taggactcga tcaaagcatc gtcgacgaac aactgtggct gcccggcttg
    10501 aacctcgatg ggcatatcgg cagacgctat caatgtcgat gctaataaga gtgtgtgaat
    10561 gcgactgagc aacataaatc tccgttttct ccgcgggtgc ggttctgtaa gagcaatcat
    10621 cagccgtcaa gctcagaagg aataccgtaa agccatttga atctcgcggg ggcgcccggc
    10681 cgtgcggata acgccgaagt tcgcggtgcc cagggtcgaa ccgggaaagc cgaagttcgg
    10741 atggttcagc gcgttgaaca tttcgaagcg gaactgcagc cggtgctcct cgtgaatcgc
    10801 aaagttgcgc gccaagccca ggtcgatcaa ctgggttccc gggcctatta gaatgttgcg
    10861 tccggcattt ccgaagttgt actgcgctgg cgccgtgaag gcgcgggtgt cgaaccagcg
    10921 ctcgatgctg cgttcaccgt ccggcaagtt gccattgctc gagccactga cgttcgggtg
    10981 caccgcacca gcaccggcgt tcacgctgtc gccgcccacc agcggtgtga atgggaagcc
    11041 ggactggatg ttggccaccg cgtttacctg ccagccgcgt aaggccaggt tggccgcacg
    11101 attgccgagg tcggggaccg gcagctcata cacggccgcg agcgtgaagc gatgacgcag
    11161 atcgaatccg gcaagacctc gtgccgccgc ccggttgtcc ggatcctgaa tgcccccgcc
    11221 cgccgactca gcttcgcccc cgtgatcgtc gattgccttc gaccacgtgt aggtgctcag
    11281 caagttcatg ccccgcgaga atcgcttctc gatcttcgcc tgcaaggcgt gatagttcga
    11341 cgatccatcg ctgtcatgga agctcagcgc gccaaacaat gggtaccgcc ggcgcggttg
    11401 cagggtaccg ggacctggcc gcgccgaatt gccgttatac gagcgctgca gccggagcgt
    11461 gcgattcccc acgtagccaa cgcttgcacc aatattcgcg ccgagctgct tttcgagagt
    11521 gagcgaccac tgttgcgtat aagggttacg gcctgtgccc agcggcacca ccgaggtgga
    11581 caatacagcc ggaatcaact gctgcggatc accgagacga gttgagccaa attgtatctc
    11641 cggaatcacg ctcccgctcg attgcaggtc gtcgatagca aatggcggga ccgctgccga
    11701 aaacaacagg ttctccgccg tacgcgcagc ccagaaaatt ccgtagcccg aacgcacgac
    11761 gaacgacgca tttcgcagag gccgccacgc gaggccaacg cgtggagcaa aaccgttagt
    11821 cgcggcctgc agatcattcg agtcgccttg gcggtttgga tatggaagag caaacggcat
    11881 ctcatagtcc ggagcgtaga ccatctgacc agtggccgga tcgaatgacc ccacctggtt
    11941 gaatttctcc ttccaagctc cgtcgcgctc gaagcgcaag cccaggttca gtgtcaactt
    12001 cgaggagacc ctccagtcgt cctgaacgaa ggccgaccag cgcaggttcc ttggctgctg
    12061 cccgtggaac cgggacacat cgctcaggtt ctgggccgaa ggcagcccaa gcaggaagtc
    12121 ggcaaagccg tcggtgtggc ggccccccac cgggccggtg aagactccag agaacgtata
    12181 acggccacgg ccccactgcg gctggaagtt gtttgccgtg tatcgccttg catcgccccc
    12241 cacgcgaaga ctgtgcgcgc cccgaatcca gctcacgctg tcggtgagtt ggatcgttgt
    12301 ctcctcacgg tccaggggaa ccgcggcatt gccgcccagc cgggcgagcc cgctgatccg
    12361 cacatctggg aagccgtcgg cgattgtgcc cttcgccagc gatggcagat tcagcatatc
    12421 ggtgaagttc tcccccgcgg tctcgtgcag aaaaaggaaa tgataccggt tcattccggc
    12481 gcgaaattca ttaatgagcg tcggagtcag gctgtatgta tgctgcgccg caaacgagcg
    12541 gtggcggttc cgcaggatat cggcgtcgag cggaaacgcg ttgttcgtca cgttgtccgt
    12601 gttgtaatac gaaaaccggc ctgtcaacca ttgcttgtca ctcagctttt gatcgacgcg
    12661 cacaggcacc tgatcggaat cgtctgtgcg agtggcaata gttgtaaagt tgcgcgctga
    12721 gtcatctgtg ttgggcagcg gaatgtactc gagaatgctt ttcgaggcag gatgaattcg
    12781 attcgatggt atctggttac cagcgaaggg ctgccgcacg gggctttgcg gcgtgtacgt
    12841 caacggatca tagatgatgc cgctacccgc gtaattgccc gttcgctgcg ctgcggttgg
    12901 ggccagcgag cccgtagccg cgctacgccg tgtccgcctg ccttcatagt tcacgaagaa
    12961 aaaggtgttg tttctagaaa tggggccacc gaggctggcg ccgaactggt tgaacaccag
    13021 tggcggtttc gcccttcgcc ctgtctccgg cgcgacgggt gcgaaatact cggtagcgtc
    13081 gaagttatcg ttacgcagga attcgtaagc cgagccatga aactcattcg acccgctctt
    13141 ggtttcgaca ttcacgactg cgccggcggc gcgaccgaac tccgccgagt aagtgctcgt
    13201 ctgtaccttg aactcactga tcgattcgat tgacggtact accacgacca tgccgaatag
    13261 ctgagcgttg ttatcaactc cgtcaagcat ccagttattc tggtcctgcc gctgtccatt
    13321 gattgtgagc gagtgaccgc cctgacgggt acgaaccgtt gtcgcggcgg gcggacctgg
    13381 cgtggcgccg ggcacgagtt gccctagctg cacgaagttt cttccgttca gcggcaactc
    13441 tcgtacctga gtcgcgccga tcacctgtcc gagcgctgcc gtttcggaat cgaccagcgg
    13501 cacgctcgct tcgaccgtca ccgtctccgc ggtttcgccc acgctcaact gcgcgtcaag
    13561 ccgcgcagtc tggttcactg ccagttccgc actgctttcg aacgtcttaa agcctccgtg
    13621 ctggattatc accgagtaca cccccggcgg caggttcgct actgtgtagt cgcccgtttc
    13681 attcgtctgc accgatcgtt ctagattcgt agcaacattg cgaaccgcga cggtggtacc
    13741 cacgaccgcg gcgcccgatg gatcgctgac aaagccaata aggcgtgccg ccgtgccttg
    13801 cggatagaca gggcgcgtaa ggagaagcag cgcgatcagc gccgctggaa ctaaccgacg
    13861 ggtggagagt ttcatgatgt ctccgattgt gatattgtcg acaatagatc attattgagc
    13921 cattgtcaat agaggttttt ctaaaattat attgactgat tttgcttgta ggattgtcga
    13981 caatgcagta tgatacaaat ttgctcaatt gctatgggca cttcaacgtt cggaaaacta
    14041 aaacaggagt caacttccga tcgcatttat gcggcgatcc gggaggccat cttcagcggc
    14101 cggctgaaga cgggccagcg tcttactgag atccagatgg cgaaggaatt cgaggtcagc
    14161 cgcgccagtc tccgggaagc cctgcagcgg ctctcgcacg agggtcttgt cgagcttaac
    14221 tcctataaag gcgcgcgtgt gatcgaactg actccggagc aactcgatga gatgctcgca
    14281 cttcgcctgc tcctcgaagc ggaagcggtg cggcaggcca aacgacgcct cacacagacg
    14341 gatcgcgatg tcctgtacgc gatcgcgcgc aagctccaca atcctggcgt taacccgcgt
    14401 gcatatccaa ccttcgactt cgaattacac cagactatct gggagatcag cggcaaccgc
    14461 accttgcagc gccacctcag tctcctcgtc tcgccgctgt tcgcaatggg cacgatcctg
    14521 cgctactctg agagctcacg cgatcgctcc gattcgacgc gcgatgctgg cgtcgaccac
    14581 atctctctcg tagaggccat ctgcgacggc acagaagaac gagccatcga gttgatgcag
    14641 cggcacattc tcgataactg gaaagtaact cgcagccgga tcgagtcatt catgaagcag
    14701 gactcttgag cggcgcacgt gatcctatgg agactcactt gcagaagttg aaaggcgtca
    14761 tcgtcccggt tgcgacgccg gtcgattcag ccggggaccc cgaccttgcg gctctgcgac
    14821 gcctgctttc ctttttgctg gcgcattccc tcgacggtct gttcgctaat ggctcgatgg
    14881 gcggctttgc gcacctgacc gatgcttcgc aatatcagat catcgaaaca atcgcggcgc
    14941 aaccgcgggg cgtgcccgtg gtcgcaggcg tgtcggatac gagtatctct cgtgtccgcc
    15001 agaagattgc cgtgattcaa accatgcccg tcgcggcgct cgtgctgctt cctccgtact
    15061 actatatgtg ccggcaggac gaactgatcc gcttcttctt gctcgccgcc gatttcgcac
    15121 agaagccgat cattctgtac gacaatccgc gttacacgca gaatgcgctc gaaccggcga
    15181 ccatcgccgg gctggcgcgt catcccaaca ttcacggaat caagatcagc gccccggacg
    15241 tactgaagtg ggaacgggtt ttggagcacg agctacctcg agaccgtttc tcgctcgtct
    15301 gcggagccga gaagctgatg gatgtggctc ttggcatgag tttcgacgga atcacaggcg
    15361 gactgcacaa catcacgccg ggtctggcag tgcagcttta tcgcgcggca tcatcggggg
    15421 atgccgcgac tgcgtcgcga ttgcaggcgc agctcaatcg catctatcag gtctttgaag
    15481 ccgacggcgg atggcggggg cttgaagtag ctttcgcggc cctaagcatg ccggcgctct
    15541 tcgcgccgcc gccgcatgac atccccgtcc ccgaagagaa gaggcgtggt attctcgaaa
    15601 ttctcgagtc ggagaacgtg ctcgcggcaa atcccgcgtg agatgggcgg cccgctcagc
    15661 actgatgtgc tcattgtcgg tggcggcgcc ggcggctgtg ccgctgccct cgcggtctgc
    15721 gaagcgggaa ggcgagtcat tctctccgag gagagcccct ggatcggtgg tcagtttaca
    15781 tcccagggtg ttcccgccga tgagcatggc tggatcgagc agttcggctg cacggctcgc
    15841 tatcgaagat ttcgtaactc aatccgcgaa tactatcgga gtcactatcc actgactcac
    15901 ttcgcccgcc tccatcccta tctcaatcca ggcaacggct gggtctctcc cctgtgtgcc
    15961 gagccgcgct gtgtactcgc tgtactcgaa tcaatgcttg agccgtacga ggttgctggt
    16021 catctccgca tcctgcgcca ccactccatc gcatccctcg acatagacaa cgatcgtatc
    16081 cgggcagcgc ttctgtatga cagcgaaacc ggtgtcaggc gcgagattga ggcgcgattt
    16141 ttcctggatg ctagcgagct cggcgatgtt ctaccgctcg cgggggtgga acacgtcagc
    16201 ggcgtggagt cgcaggcgat gaccggagag cccggtgctc cactccaacc ggagccgcgg
    16261 tcgatgcagg ccttcacctg gtgtttcgcc gtcgattacc tctcggatga ggaccacacg
    16321 attgaacgcc cgcgcgacta cgatttctgg cgcgattacc gccctcgtct gtcaccgccc
    16381 tggccgggcc cactgttcca actcgccggc ctcaacccac gcacgctcgc gcccgtcagc
    16441 tatacgttta atccgcacgg cgagtcaggg ctcgcattct ccggcctctg gacttatcgc
    16501 cggctcctcg accgcatgca attcgcgccg ggatcggttg cgagcgacat ctgcctcgtc
    16561 aactggccca tgaacgatta catggaaggg gatctactcg gatccgccgt tgaatgcgcc
    16621 aaccatctgc accgggcgca gcagcagagc ctgtcattgc tctactggct ccagacggaa
    16681 gcaccacgcc cgaagggcgg ttacggatgg cgcggactgc ggccgcgtgg cgatgtactc
    16741 ggcacggcaa cgggcctcgc actccggcca tatatgcgcg agtcgcgaag gatccgggcg
    16801 attcgaacgg tcactgaggt tgatgtctcc gccgttcatc ggcccggcgc cactcgagcg
    16861 atccattgcc gcgactccgt cggcgtgggc tactatcgca tcgacttgca tccctgcacc
    16921 ggcggtcaca actacattga cgttccggca ctgccctttc agatcccgct cggatcgctg
    16981 attccggtgc gtatagagaa tctgctggca gcgggtaaga cggtcggagt aactcacatc
    17041 acgaacggtt gtttccgtgt ccatccggtc gagtggaaca tcggtgaagc cgcaggcgcg
    17101 cttgctgcct attgcctcca ccggcgtatg gttcctcgca gcgtgctggc agcgccaggc
    17161 gacttccagg acatgctgtt tcgacagggc gttcaactcg cctggcccgc cgaactcaac
    17221 ctggacgacg gagagccaca cgtccatgcc aggccgactc gtctcctctg agttgaagcg
    17281 agcacactcc aaagtgggcg gcgtggtcgt cggcactgcc atcggcttct gaacgggtgg
    17341 aagaagggca gcggccgttc cttcttgatc tgctgccaga cacgtattcg cggcgagaca
    17401 agcgaagatc atacatgtaa gcggctggcg atgaactacc tcaagcggct aacgggcgcg
    17461 atgaaggaga atcaactcaa cccccgcaga gctaccacga cgtgtgtcga gcaggtttca
    17521 gcagccgcac ggcgttctcg cggaggatgg cgagtttgtc tgcatcgctg atttcggcgg
    17581 cttctatgcg ccgtctctgc gcggctgcgg aatagagcgg cgtatcggtg ccgaacagga
    17641 tgcgacttgc tccgatctcc tgcacggccc actcgatcag ccccgcgtag atgctgctgg
    17701 cgctcgaagt gtccacgtaa atgttgccgg cttccgaggc ctgaatggcc cgcacctggt
    17761 ggcccaggtc tcggtcccag ccgcagccca ggtgcgcgag gatcaacgtc acttcgggaa
    17821 accggtcggc gaagccgacg cagtctttcg ggaggctgtt ctcctcgccg ctatgggtca
    17881 gcacgatggc tcggtgccgc gcggcgaact cgaagatcgc accgccttcc cgcgcgatgg
    17941 gatagccgtg ctcttccggg tggattttaa tgccggcgca cttggggtga cggagacgct
    18001 cggccgcctg cccgaaggtg gcgggccgac gcggatcgac gatcacccat tgcagcagcc
    18061 cgggagtttc ctccaccaac cgcgcagcct cctcgtttcc ggctgccgcg tccgcctcca
    18121 tgcgcggaag caatccggag agcggcgaaa cgatggtgag ctcgatgttg gccgtcctgg
    18181 cccgttccag caccttttcc acgccgccgc tcatgatccg gttctggata gtcaagccct
    18241 tgcggcagta cagcccgtag tgaccgtgaa cgtcgatcgc agcgatttcg gcaatctcca
    18301 tagctccttc ccagagcgca gccaggttat ggctcgccgc gactgcgaag acccaggagc
    18361 aggaatcctg ccgggtccgt atcggcagcg gccgaagtca gacgcaggta gctgatgcca
    18421 cgaaacgcct cgagacccag caggaatgcg acaaaacggc cgtcctgata gagccatgcg
    18481 acattctgct cggcgtccca cttcaccgtg aggtccagcg tccggccggt ttccgcgcgc
    18541 gaggccggct cgctgagcag aaggttgatg accggcggat tggccggaag gtagggtccg
    18601 ttgttggcgt attgcacgcg ccaccgaaga tcggaaccca ggctccagcg gaagacacct
    18661 ccctcccggt gatacgggcg aatggaatac tccgagagcg tgaagtcggc gcctccgaag
    18721 ccgggatcca gccgcacctt catgttgacg ctgcctctgc ggccgaaggg aaagttccag
    18781 gtcatcccgg agggcgggag cggctcgccg atttcgaagg cctgcgtgcg ttgaatgcgg
    18841 acggcgggcc ggtcctccgg tccgtgggtt acctcgaggc ccttcgttcc cacggtgtaa
    18901 agcccagcaa cgcccgctga gaaatcttcc gcggaggcgg tttcccgcag ccactccggc
    18961 cggacgcgaa acagccgcac ccccgccctc cctccgacga cgcggcgctc gttgtagccg
    19021 aggagtatgc ctccgtcctt cgcctccagc aggaaaggat atcccactga gctgcgaatg
    19081 tcctcgctgg cctggatagg agcactcacc tggcgatacc cgcgccatcc cgcgccgtca
    19141 tcgatcgcca tcgccatcac ctgccggcaa tagctaacgc cgtggaacag gttgtagtcg
    19201 aactcgttgt tccaggcgat gaccgtggag ccgttgcgta gcctcaggac cgcaccgggc
    19261 gagttggtgg cccggaagac ggacttgcgg gctggggacc acgtcgcgcc gccgtcggag
    19321 gagaaagact cgaacatgta gccggcctgg gtgcgcataa ccatccacac gcgcccgtcc
    19381 ggtagctccg cgaccaccgg ctcgatcgct cccgactcga cgatcttgcc gccactatcg
    19441 acggtgaccg cgctcggcga tcgcttccat gtcttgcctg cgtcgtcgga atagaccacc
    19501 gtggaagcga aggcgccgct ctcgcgatgc gggacgacaa gatagcttaa cggaaccagc
    19561 aggcgtccgg aggcaagttg gacgacacta ttcagcgcgc cggtgtattg tgcgccgtag
    19621 tcgatggacc gtggctcctg ccaggacctg cccccatcgc gggacacggt atgcatcagc
    19681 agcgccgaag tcttgctcca atcgaccggc cggtcccagt cgagcttctg aaggtagaag
    19741 atgtgcaggt taccgtcacg tgtgcccaga gggtaggccg cgtggaggat gcccggcccg
    19801 gtgggcgaga acaggacctg cgggtcgctc cagccggccc cttcgtcgga ggaaaagcgt
    19861 cccaccacat gctgcgggat ggagacatcc ttccattcca gggctggccg gctctcgaga
    19921 tagacagcga aaagtcgtcc gtcggccaac cgcgaggtga aacccagccg tcctttgaac
    19981 ggcagttcca ccggtccggg ggaagaggcc gcggcaaccc ccgcccagag ggcgagcacc
    20041 accagcggcc cgtgcatcac gagaggtatt cgcatggcat tcaaacggtc atagcgcatc
    20101 gttaaaagtg cagcttcagc gcaaactgca ggtcccgcgg atcggccgcg cgcgtggcca
    20161 gcgcgaagtt ggccgagtcc ggccgggcat tcagatcgat caggtacggg tggttgaaga
    20221 tgttgaacgc ctcggcgcgg aattccagcc gcagcctttc ggtaatgggg aattgcttga
    20281 acaggccggc gtcgatgttc cacgtgctat tcatatcgag cggattcatc cccgcgttgc
    20341 cgtaggtaaa gttcggcgac gggaagtaag cccggtcgta cttgggccgg tccacgccct
    20401 ggccgccgat ggtgtactgg ccggccgtgc cggttacgtt cggcaggcag aagttgccgc
    20461 ccatgttcga attgcagaag ctctgggcga ccgaccacag cagcggagca ccggtgtgcg
    20521 cgtacagcag gccggtaagc tgccagccgc cgatgacgtg gcccagcggt ccgccgatgt
    20581 cgaacagccg cttccccggc ccgaagggga ggtcccagac ccagtagccc gagacgacgt
    20641 gccgcatgtc ttcgccggtg cggctcactt cgttcttcag gtcgtgcgga ttctgcacgc
    20701 gcgagagcag gccctggtcc agccagcctc ctccgcggct cagcgcgtgg ctccaggtcc
    20761 agtttgcctt gaagctgagc ccgcccgaaa aacgcctttc caccgcgagc agcagcccgt
    20821 tgtagttggc cgttccggcg ttgtgatagc cggtgatgga tccccatttc ggaaacggga
    20881 tcctgtcggc gagaggcagc gagcttggcc ctggcgtctg ctcgtcccac gcggcccaca
    20941 gcttcgtggc cttcgatccg acgtaatcgg ccttgaacac cgtgttggga agaatctcgt
    21001 gcgcaatccc gacgctccac tggctgacat aggggttcag gttctggcgc tgctgcttgg
    21061 cgttgaccga gaaggcgacg ccctgctggg tcacacctgc gaaagaaccg gcataggccg
    21121 actcccaggt gagcgtcggg gtggttgccg acgaggtgag gcttccggag agcttgttgg
    21181 gtggaacagc cgcccaaccc gccgcctgcg gcaagcccag gttgacccaa tactggagcg
    21241 cgtacccggt tcggatcacc gtcttggtgc cgccgaaggg acggtatgcc aggccgaagc
    21301 ggggtcccca gttgttcttg tccgcgtcgt agatggtatc ggaatccacg aagccgaagg
    21361 gcaagtcggg ccggaagttg gtgtagaact cctgcgcggt ccggttctgc cgcgggtatc
    21421 gcaggcctcc cacaccgttg cccagggtgg ggtcccattg cacgaggtgc ccggactgct
    21481 ccaccggagc cgaggtcaac tcataccgca ggccgaagtt gagcgtcagc ttgggagtga
    21541 ccttccacga gtcctgcacg tagccgccaa aaaagcggct actcaggtct ccgcgatgcg
    21601 attgcggaaa gctgatcgac atgctgaacg ggttgcccag caggaaatcg gaatatccgt
    21661 ctccggtgaa ccggccagta aacgaaacgc tccccgcggt ggtatcggag aggccccagg
    21721 cgcggacagc ctgcgtttcc gcgcccatct ggagagtgtg cttgcctcgc acccacgtca
    21781 ggtcgtcgcg caggggatag ttccagttcg cgttgtcgta gcgatagagg ttcaggacgc
    21841 tggtgatcat ggaggtggta gacatgctga gggagaccat ggccacgccg gtcgccggat
    21901 cgccccgcag cagcgggtat cggagccccg ttctttccag caggtcactg atgttctgtc
    21961 cccggagata gtccgtgctg cggttgtagt tgagacggaa atcattggtc aggctggggg
    22021 tcaggatgct ggtcaggccg agcgctccgt tctgccagtt gttcgtgctg tgctgccccc
    22081 ccaggcgggt cagtccggaa gagttcgccc ccgggttgaa gacgtcgagt ttggtgatgc
    22141 tccagcggcc gaacagcgaa tgcgcgtcgc tcagccggtg gtcgatccgg atgctgaaat
    22201 tgtcggcgtc gtaggatcgg gacggcgcgg ccacgtaatt ccggaagaaa tcggccgggt
    22261 tggtgggttc cggaaaatag gcgtcgatca gggtcgcgga aatgctgttg atccgcgaca
    22321 ccggaatggt gttgtttggg aaaggctggc ccgttgtggg gtccataatg ttcttgccga
    22381 tgaccgccgc gtaattagag aggtccccgc gccgttcttc tgccgtgggg atgcgggtcg
    22441 tataggtgtt gcctttgcgc tcccgtgtgc cttcgtagct ggcgaagaaa aacgtgcggt
    22501 ccttcaacac cggcccgccg aaggtgaagc cgaactggtt ccgtgtcagc ttcggctgct
    22561 ggcgccgtcc gctggcatcc accgttgaaa agaagttagt ggcatcgaaa tcctggttgc
    22621 gcacgaactc ccacagcgtg ccgtgaaact cgttggtgcc cgacttggtg atcgcgttga
    22681 ccacggtggc gccgccgaag ccggcggccg cggagtacaa cgccgcctcc accttaaact
    22741 ccgcgatcga gtcgatgggc ggcaggacgt tgtaactgcc cgaccacatg tctttgttgt
    22801 acgtgccgtc cagggtatag ctgttgccca cagatcccgc cccggctccc gtcggcgagg
    22861 gctgcttcgc cgtgttgtag ttgtagttgg gtggccgcgc gtcattcgaa cggttgaccc
    22921 ccgccgtcag gctggccaac tgggtgaaat tgcggccgtt caacggaagc tgcgtgacgg
    22981 cctcgctggt catcaccgct ccgatttcgg gcgtctccgt atccacgagc ggtgcttccg
    23041 ccttcaccgt aacagattcg gagacctggc cgacctgcat cgtgacatcg agacgcgctt
    23101 tctgtgccac ctggagcacg acgccctcga gcgcctgccg cctgaaaccc tgcttctcca
    23161 ccgtcactgt ataccgccct ggcaccaggc cggacagatc gtaaattcca aactcgttgg
    23221 tcacggtgac ccggttcagg tttgtatcca cgttcgtggc tacgaccttc gccgcctgaa
    23281 tcactccgcc cgcgggatcg gagactgtcc ccaccaaagc actcccggct acctgtgcat
    23341 gcatcccagc cggaatggcg aagaaagcga aaccggcaaa caatgccgcc acggcggcct
    23401 ttaggctcga catctcccta gtcctccttc attcggaaac cttgcgtgtc cccgaattaa
    23461 gccgatgcta cagtagacat caacatatgt caacaaaaaa ttgtcaacag attacatcac
    23521 ccgaagcggg acactttcta tgggcgggtc agctcctcga tggggatgag ctggattctg
    23581 ttatttcctt gcgtccggga tggatccctg ggatccgcag ctaacccgaa tttcttccgg
    23641 aactcatcca gggtcatgcc gtagaaccgc cggatcattt cgggggcgcc cggggtgccc
    23701 actccgtaag tgaccacggc attgccatcg tggaatgcgg tgaacgggtg atccatgcgg
    23761 agcggcccgg gcgcgcgatg gtaccgggcg gtgtcggtcg gctgcttcca ggtgcggttc
    23821 tgcagatagg gcttgagcgg ttccggatcg atgcgggcga catcgtccag gctctcgagg
    23881 ttccggaagt tgcggaaggt aagaccgccg tcagcggtca ccgcgcagct caggcgatgg
    23941 cgcgggtatc cgctcaaggt ctcccaacgt gaagcctgcg accagactat gagcacgtcg
    24001 ttgctgccgg gaattcgctt tacggacacg gggcccgagc cttggaggtc ggtcggctcg
    24061 gcctcttccc acgttctgcc gcggtcccgg gagtaggagc ggaagatccg cccgaggccg
    24121 gtgaagccga gcatgagcag gcgcccgtcg gaaagctcgg ccacgccgta ttcgagcagc
    24181 ccgtagatac caccgacccc acggtcgatc gtggcataga cctcgttttc gctgtggtgc
    24241 caggtcttgc cctcgtcgtc cgaccagtag gccgtcacag tgtgcaggac aggcgcgtag
    24301 gcgttgcgaa agctctcgcc gaagagggtg aaatagtggg cgctccggtc gcgcggggtg
    24361 gggccgacgc ggtgcgcggc ggggatgagg agccgcccgt cggaaagcgc gctgagctgg
    24421 tcgaagtagg gatagtcgtt ccttcgcagg gcattgaccg gcaccccctt cgaccaggtc
    24481 cgcccgtcgt cgtcggagta gtggaaggtc gaggtgatct gggagttatc ggcgttgaaa
    24541 ttgacttggc tatgcgcctg caccatcccg agtctgccgg atttcatgcg cgccagggag
    24601 ggcgagagaa agctgatcac gggagccccg ccggcggtca ttacgggctg aggatcggtc
    24661 catgtacgcc ccccgtcgga ggaataattg acctgcatcg gatagggcgg tttgtgattg
    24721 cctcccacgc cccaaaccca catcaggcgg ccgtccttca acgagatcat gctgccgcaa
    24781 ttggggcttt gataaacctt ggcgtgcggg acggtgatga cttccgcacc cgcgccatgg
    24841 gatggagaga gcgccaggac agcggctagc gcgccgagcc gaattgacag attcactacg
    24901 atagctcctt tcatggttgt agagcggtgg tggatggcgg cacaaagagc tcgcaaaacg
    24961 cccgcagcac gagctctccg gcctttcgca tctgggccgg attctttccg gaccaggtaa
    25021 aggagggcac cgccacgcgg gtcccccagc ggcgcatggc atagtgccgc agatccgcct
    25081 cgcggccggc actgcccgaa gcgtcggtga gctgccagcc tcgatcgaaa aagccggtct
    25141 tctcaagatt caggaacagg cgggcgcggg tctccggttc gggcataaag gtccggatcc
    25201 aatcccggcg ccacatccac tggtggtagt ccaggtatcc ggccgggcga atgcggtcga
    25261 ttaggttctt cagggcggct gaactcggat cggaaccgat gcggacgagg tccgcgccgc
    25321 cggccgcggt caggtgcgag gttcccaggg cgacgccgtc cggattggcc atcggcacga
    25381 cgtggaaagt gtatttatcg agcagggaag ctcccgccgg gctacaaagc cattggatgg
    25441 cgccctccgc cgcaaagctt cccgcggttt cataggggtg catccgggag gtcagcagaa
    25501 tcgcctttcg cgtttgcgga tctccccgcg caatcctcaa tcccacgatg tcgcggcccc
    25561 cggcgctgcg ccccaccgtg atggtctcgg cgcatccttc gggcaggcgc cgaatggctt
    25621 cccgcagctc cgatgttgtg tacttggggc tgaggccgag gtagctgctc ccgggcggca
    25681 gcgaaagagt aaacctcgct ctcgatccct caatggatgt tgcctgatgt gccttccatt
    25741 cgtcgccgcg gccgctcatc aggacgctgc gatagagcat ccaatgcggc tggtcttcct
    25801 tcggctcatc ccattccaca tccaccacca cttcgagcgg ccgtctggcc ggattgccgg
    25861 ccgcgaccag aaggttcagc ttgtggtcgg ggttggacgg atcttcgctg aagggacgaa
    25921 tcagaaaccg gttgggcccc tgccgcagga tgctcccggc acggttcgga ttggcaccct
    25981 cgaagccgcc ggacagttcg gcttccaccg gccggctgcg gagctccatc acccggatcc
    26041 ccttggcatc gaggtccgcc gccgagcgga tccgcagata ggcaagaccc gacgcctctt
    26101 ccatccccac cagcgccgcc acgaagaccc cgtcctgata tagtgtggcc gtattggcgc
    26161 cggcgtccca ttcgacgctg acttccagga cgcgtcccgc ctccgccacg gaccggatct
    26221 cgccgaaagt caggttggga tgcggcccgg gcacttccat gtatggtccg ttgtccgcat
    26281 attggatcct ccacttgaga tccgcgccga tcatccaccg gaaggtgccc ccctctgcgt
    26341 ccgaagggcg aagggaatac tccgagaggc tgaagtgcgt accgccgaag cccggttcca
    26401 ggtacagctt catctccacc atgccggctg ccgcgagggg gaaatgccaa gtgaagccgc
    26461 agggctcctc cttccgggtc ttactcagca gcagtgcagg atcctcctcc ggacccttgc
    26521 gcagggcgac gccgccggtg cccgcgaggt agagattctt gagaccttcg gtgaagttct
    26581 cgctcgcgcc cgtcactctc agccagtcgg gatgtatctt gaacaggcgg attccggggc
    26641 gcgccccctg cctgcggcgc tctctgtacc ccagcagaac cgtcccgtcg ccggggtctg
    26701 caaggaagct ataggtggcc gtcccgcgtg cgtcgtcgtc cgccctgaag ggagggttca
    26761 cctgccggta gcccttccac tcaccgttct cctggagagc cagggcaacc gcctgccgcg
    26821 agtagctcac gccatggaac aggttgtagc cgaactcgtt gttccaggcg atcagtaaag
    26881 gcccgttctc caggcgcaac accgaagcgg gtgagttgga cgagcggaag cgtgcgcgcc
    26941 gcgcaactcc ccacgttacg ccgccgtcct ccgagtgcga ctcgtgaaga tagccggtct
    27001 gggcgcggac gaccatccag acgcgcccgt ccgccagttc aaccgccacg ggctcgatcg
    27061 cgccggactc gcccactcgt ccgccgctgg ccacgtatac ctcgttcttc gactcgcgcc
    27121 agttctcccc gccgttgtct gagtagactg cggcggccgc atagcggccc acggggctgt
    27181 cggcattggc ataggcgaag ggcaccagaa tccggcccga ctgcaactgg atggcggact
    27241 ggagttcggg aatccaacgg cgccccagcc cgatttgcgc cggttcgcgc cagcttcgtc
    27301 cgccgtcctc cgagacggcg tgccacaccg tggaggacag cttgccccag tcgtacggct
    27361 gctccaggtg aagcagttgg acgaagaaga tgtggatgcg cccgtcacga gttcccaagg
    27421 gacgcacgct gtttagagtt cccggcccct gaggctcgaa cagcagttgc ggcgtgctcc
    27481 agctcagccc gtgatccgaa gagtaccttc cgaagatccg ctgcggcgta cccgtatcgc
    27541 gccagcgctc ccgcgttccc cggctctggc ccgtcgcggc gaacaaccgt ccgtccgcaa
    27601 gacgcgtaat cgggcccaat tcattttcaa aggcgatctc ctggaaacca ggagaggtgg
    27661 cgagaatgat tcccagcatc ggcagcaaca tggcgcgggt gtggctccgg aaggtggatc
    27721 atatccgact ctgcaataat tgtcaacaga ttagtattga ctgtataacg taaactgtct
    27781 acaatttttg gcgtgggatc gcacgagctc gaggtggaag ccagcggggt catcttccgc
    27841 aaccctttcc ccggccacag agtgattaac gccttttatc cgatgattgc gccgctggac
    27901 gacggggagt tgctgtgcgt gctgcgtcgg ggcggcgctc tctattctcc ggatggaatg
    27961 ctgcacctgt tccggctggg gaaaggctgc gatacctggg cgcaccaggg acctgttttc
    28021 gatcgcattc gcgatgcgcg acagtataac tacagcgacg gccatcttac gcgcttgggc
    28081 gacgggagtc tgctgctgcg ggtggcacgc tacgacatga cggatccaga gcggttcgtg
    28141 atcaacgcgg ccacccaggg cctcgcctcc ctgcaaacgt gctttctgcg ctcctacgac
    28201 gccgggcgca cctggtcgga accagtggtg gcagacgtga aagagcactt cgcgccgggc
    28261 gtcgagccgg cgccgttggg cggggtggtc gagttgccgg acggctcctg gtttcagctc
    28321 ttcgacacgt ggaaggacta cgaaaacggc gggctgttcg acgtgaacgg ttacggcctc
    28381 ttctccgccg acggcggccg cacctggcgg gacaaagttg cagtggcagt ggggaccgcg
    28441 gaggagcgct cctactcgca cggtatcgcg gctcccatgc gggacgggcg cgtgttcgtt
    28501 tcctactgga cggcgacgca ggatcttcag acctccttcg accttcacac ggtcatctcg
    28561 accgatgcct cggcacgtca gtggagccgc cccctgcccg ccggcgtccc cggacagacc
    28621 agttgcgttg cggatgccgg aaacggatgc atgttgatga tctactccca ccgggaacac
    28681 acggagcagc cgggaatcaa agtggttctc tcgaccgatg cgggccgcac ctggcgaaga
    28741 gacgaacctc tggtggtctg ggacgcttac ggcaaagaat cgcttggcgt tgcgcgcagc
    28801 aacacgtacc ccagcagcca cgacgccatc gcatacggcg cgccgcgagt ggtggctctc
    28861 gatggcggca gcgccatcgc cagcttctgg tgcagccagg gaggggacac acattgccgc
    28921 tggtgccgga tccgaatccg ctagggtttc gaccccgcgc ctcgaggaag tgtgcagacg
    28981 agcatgaggg cccccaaggc cgcaacggcg gcaaacacga agcttgctcc gaacccatag
    29041 cgcacggtga ggagtccatg cagagcgggg ccgaagctgg ccgcgggcgt ggccagcgcg
    29101 agtacggctt gattgcgcgc gccggccgcc aggagagaga ctccggccac gtagttcggg
    29161 aagtaggcac cgcccagttc gcctgctccg agcagggcga aagccaaaag gaacaggtag
    29221 ccgggaacca ttccggccca cgccgcagca gccaccgtca ggagcacagc ggcgaccacc
    29281 ggcgcccgcg ctccccggcg caggatcagg ctcgccagca gaaacccggc cgccgacttg
    29341 gaggcgaacc gcaaggccat aatgagcccg gaaagctcct tcggatcgcg ccccagcagt
    29401 tcccgggtat atagagagaa gttgggagcc gcgtaaacgg ccgtgaacca caggacgtaa
    29461 gccatccaca gccggcggag cggccggtcc gcggcgaaag aacggacgct cgcggccacg
    29521 tgttttaaca tgggcaaacg cggcggttcc tcggcgggcg gcagcttgaa ccggagcgcg
    29581 cctgcggccg ccaccgccag gcacaccgcg ccgaacgaat agagcagagc gaaatcccgc
    29641 ggataggcca gcgcgggaat gccgccttcg agcacgtact gtgcgcccag ggagccggcc
    29701 acggcagcaa tggggccggc tccgaaggcc catcggaagc cccgcgccct gccggcctcg
    29761 ttggtgccac ggcacagaca ctgcagcatg aagacctgcg ccgtagaagc cgtaaccccc
    29821 atggtgcatc cctggccgat gatggcggcg atgcgcaagc tgttcccggc gggcaaccac
    29881 agaaccgccg ccacaagcgt cagcatgacg cagctcaggc cgttggcagc cgccgcggcc
    29941 atgcgctcgt aacgttgcgg gacggcccaa gccaccacca gcggagccgc gaaccccagc
    30001 aggtacagag atgccggaag gttcgccacg gtgggggagg caccgagctt atcgcagagc
    30061 gcagcctgca caacccccac atacgtaacc ggagccgcca ggtacaccag gcacgcccag
    30121 agcgcataga tgcgactgtt gacccgatca gcttccggcg gcgccgtgtc ctcttggact
    30181 ccggaagcga cgttcatacc tcttcgagga actcgggcag gttgcggcac agccactccc
    30241 ggcacaactt cacgtcgcac tcgccggccg catcgtaggg agcgcgactc gccaggatcc
    30301 ggcctccaag gaaaccggtc gtcgcggcga tgcaccggtc aagcgcggtg tcgtaaagcc
    30361 cccgcgcgac gcacggagcg acgaactcgg ccagaaaacg cgccagcgcg gcagcgatgc
    30421 gcatggcctc ggggctttcc tgctcggcca gccggtagta gcgcagcatg aacccggggc
    30481 aagccagaac gaaggcgctg taggagcctc gcgcgccctc cttccatagg ggcgccaaca
    30541 ggtgctcgga gacaaaaatc ttcactcccg gcgccgccga tgacaaagcg gcgaattcgg
    30601 cggcatcccc cgcccccttc aggccgacaa ggggcacccc ggcatccatg attctcccca
    30661 acaggtccgt ggtcagcggc ttcttcgagc gctccgtgtt gtagataacg atgggcatct
    30721 cgggcacggc gcacgtcact tcccggaaga agcgcacagc ctgggaatcc gagagagcca
    30781 tccagaaagg aaatgcgatc tgcaccgcaa aggctcccag ttggcctgcg aactcgacgc
    30841 gccggatcac ttcgccggtc cacagcgccg tgcagcctac ctggcacgga acacccgccg
    30901 cccggctttc ttccacggtg gcccgggcga cgcggcgcca ctcctcttca cgctgggcat
    30961 agaactcgcc ggtagtgcca tgcgtgtaga cgccgtgcaa tcccgcgcga cagatccggg
    31021 cgacgttctc ccggtaggct tcctcgtcca gttcgtcctc ttcggtccag ctcaccggca
    31081 aagccgccca gagccccctg agccgctcga tgcggctttc cgcgccggag tctagaggcg
    31141 cgacgattgt tctattcatg cgttccaccc aaaccggagc gtacccggct gacgatctcg
    31201 cccttgcaca gccgctcgag caggcggccc gcctcccggg cggtcgttac ggtccctctg
    31261 ggcgagcggt cggcgaagcc cgccgtcatc aagttgccgc tgcggatgcg cgcctccagc
    31321 gtggcgatgg cgggctcgat tccggccatc cggtagtgca tcgccgtgac attgccggca
    31381 atatgcgttc tcgaaaatcc gagtcgcgcc atgtgtgcgt tgacggtcgc cttgagatcg
    31441 ctcgaagccg cccgaaggct tccgcatacc agcgcagcgg caaccagcag ccggcccgca
    31501 gccgtccatc ctccctgcct gcctcgtccg gcgcctcgca ccccacgatc atacatttag
    31561 ctattactgc ttgtcaatcg caaactgtct acaaaaaact gtataatgaa acgcaatgga
    31621 tagccgcatc ttagccttct ccgtcgcgga gtaccgcgac cggacccgcc gcgttcagga
    31681 ccgaatggcc gaactgggtc tggacgcact actctgccac agtttcgaca gcacgtgtta
    31741 tctcaccgga ctgcagtccg tcatggggcc ggccaagtat ttcatgaccg tggttcccgc
    31801 cgaaggggac ccttttctac tggggcagga tttcgagttg cacaacgcca ggttcacttc
    31861 gtgggtggag gacctgaccg cgcatccggt cttcgaagac cccgttgaag cttcccgtgt
    31921 cctcctcgcc ggtcgcggcc tggcgggcaa gaggctaggg gtggagatga agcggctgtg
    31981 catgaatccg ctgcagttcg ccagcctccg gcaggcactt ccggaggccc aacttgtcga
    32041 tgcctccagc ctggtaccgt caatccggat gctcaagtct gcggcagagc tggattacat
    32101 gcggcgggct tgccggatca gtagtcgtgc catggaggcg gcggtgggcg ccgtggcaga
    32161 gggtcgaacg gacaacgaag tggccgctgt ggcggtggag gtctgcttcc gggaaggcgg
    32221 cgagttcttc tgtctggagc cgatcgtcag cctgggagat cgttcgagcg tgccgcattc
    32281 gtcctttgcc ggcaaccggg cacgctctgg tgacctatgc ttcatcgaga tcggcgctaa
    32341 catccagcgg tattctgggc ctctcattcg cacagtggca cttggcgaac ccgacgcgga
    32401 ggtcagtcgg gcaggtgcag aagcggcggc aagcctcaat gccgtgatcg ccgccatgcg
    32461 gccgggcgtg acggctcaag cggtcgcgtt tgaaggtcgg aaagcctggg agtggacggc
    32521 gtcgcaccct gagtacgtct ggaatggcgc ctacgcctat tccgtcggcc tgggtttccc
    32581 gtccgactgg tgctggtgcg atgcttggct gttcatccac gagcgtgagg aggccgtttt
    32641 gcaaccggga atggtctttc actgcacgaa taacatccgg cgaccgggcg tgttcggcct
    32701 gggaatcagc gaaacggtcg cgatcactga ctcaggcgcg gaggttctga cctcgtttga
    32761 ccgccgcctc ctggttcgtt aatccgcatt ttgtcaacac aagactgtac ggagttggct
    32821 tttgacaaac tagacgctgc aggatacgat taagctgatg gcacgagcgt cgcttccaga
    32881 ggtccggttc gtttcgatgc gcgcgagcgt agccgatacg ctccgtcagg catttctgga
    32941 cggttatttc agcccgggag aggacatttc cgaggtcgcg ctggcgacgc aacttaaggt
    33001 gagccgcgga cccatccgcg acgccctgct gatcctcacg caggaagggt tggtgacgca
    33061 ctcacccaat cgcggcttct ccgtcctgca gttgacggac tcggatttcg agcacatcca
    33121 aaatgttcgg ctcccattgg aggccctcgc actgactctc gcccgggggc gggtgtcgcg
    33181 ggaagacgtc gaaatcctcg aaacattgaa ggagaagctg atggaggcat tccgggccgg
    33241 caacttcagc gcggccgccc gttgtgatct ggagtttcac tccgctttgt gggagcgaag
    33301 cggcaacccc tggctggagg ccgcgctgaa aaggatcatg gtcccgtatt ttaccttcac
    33361 catggtgtac cggcagaagc cgagcaccct caccgaagcg atcatcgaag agcagcacgc
    33421 gctctatatc gactacatca aaggtgagtc cgataaatcg gccgaggagt gcatgcggat
    33481 tcatctcggc tgaagcgtcc ggactggcgt cggccgctgc catctctccc ggcgtacata
    33541 actcagataa gccagcagca gcactgcgga gcacagcaca tagagagccg cgaccgaggc
    33601 gaaaatcgcg gggagccccc atgcacgcct ggcgtggccc gcgagcaaca cgcccgctcc
    33661 accggcaagg caattgagac tattactcag tcctatgccg gtgccgcgca ggccggcggg
    33721 caaaaggtgc gtgagcactg ggtgctcgtt gcagatgccc agcgaccgga taaggaagaa
    33781 gcagacgctg gcagccgcaa tcacatacag ttcacgggac caaagaaaca ccagcagcag
    33841 cggcgcggcc gcaagataca agccgcagtg cagaaggatg cgatattcag tccctttgag
    33901 ccccacggca tccgacggcc gtccgcccgc caggagtccc acgatgattc ccgtctgcga
    33961 gtagaatgta cctgcgaagc ccgcctccgc aaggcccatt ccgaacgtct cgagaaagta
    34021 gagcggcatc caggtcaaca gcacccacat cgcggtactg gccagcatgg cctgcagcaa
    34081 aatcaggcgg caggtgggat tgccgaacac gatcgcgatg gcccgcagcg ggggagcact
    34141 cgcattttct cctgcgcacg tctcgccgcc gctgtgcgcc ggcagccgct tgaagaagcg
    34201 gctggccgcc agagagagca gcagccccaa ggcccccaga accacgagcg gcgaccgcca
    34261 acccatgcgt tcccccagta aaccggccaa agccccgccc ccgaccatcc cggcgctata
    34321 tccggccgtg tggatcgcca tcgcagtagc tcgtgtagca ggcccgtgcc aagctgccag
    34381 gagggcgacc gcggccggca tataagcagc ctcgaccact ccgagcgcca cgcgcatgcc
    34441 gagcaattgc ggcgagttgg cggcgaagct ggttagaagg gtcacggcgc tccaggcgaa
    34501 caggcttccg gcaatcagga aggtgcgcga aaagcggtct ccaagatacc ccgccagtgg
    34561 agaggccaat gcgtacgacc acaggaaaaa actacccgtg gcggccagcg cgacgtccga
    34621 catgcccagg tcgctttgca gcagagggaa taccgccgtg atggcggtgc ggtcgccgta
    34681 gttgagtgcc gcggcccaga acaggagcgc caccacccac cacttgcgag caccgtttcg
    34741 atccaagagc cctccctggc gacgctagac catccggtcg aactcgaacc cgagcttcga
    34801 aaatcgggcg cggatttccg aatagtacac ctggaggccg agcacgtccg cgcccacgtt
    34861 gcagaacagg gcgcccatct ccaggtatct ctccacgttc tcggcgttca cggggaggcc
    34921 ccacgcgcgc ccgcgtttct cgcaagccag cgccacgcac tcgattacct tctggacggc
    34981 cggggcctca atctgcccgg ggattcccag agcatgggcc aggtcgccgg gcccgacgaa
    35041 aagcgcatcc actccgtcta ccgccgcgat ctcgtccacc tgttcggccg cctccggatc
    35101 ttcgatctga accaccacga atgtattatc gttggcgtgt tgtaggtact ccgggaccga
    35161 aaccgtgcag tagcggccgt cggcattgcc gccgtcaaga gcgcggcgcc ccagcgggtg
    35221 gaagcgcgtc atcttcacta cctcgcgcgc atccgcagcc gacatgcagt gcggaaccat
    35281 gattcctgtg gcaccaagtt ccagcgggtg gatgtagctc gagtagccga aacgggccac
    35341 ccgcaccagc gagtccattc gatgctgcga tgctgcacga attagcagcg ccagccgatc
    35401 ggcatccagc ggaacatgct cacagcacat ccagacgcaa tcgaagcccg ttagtccgat
    35461 aagatcgacc acagctgggt catgcgtatt gatcttggta caggctaccg gctgcttacg
    35521 cctcagtcgc atgcggacac ggctgctgca catagtttgc ctccactccc ttttgcgatg
    35581 gcatcaatcc cgggactcgc catgagtaca actctaagct gaaaaaaagc aactgtcaac
    35641 aactaacagt ttaatgttaa cgagaaaact ctgctccttt cgtgacgtcc cattcgtcca
    35701 cgtaacgatc acatcgtcgg tagcccgcat caagacaaac gaaacgtcgc ccggtgccag
    35761 gcgggcatcg tcactcgtca ggccggtggc ggcagaagtt ttggaggtgg cgcgcgccgg
    35821 cgtaaaaaga taatcgtcac gggtgtaggt gttcactccg gccacctcca gcacctcgcg
    35881 tttcgctttc gtctggatgt ggctgtagtg ctcgagccga cgccgaacga ggtgagattt
    35941 tacgtaatcg cgccgtccgg atttagagta caatcttaca aatccgaagt tcatccttgt
    36001 ctggactggg gaggactgta tgaaggcaag tcgccatcta ccgctgatcg tctttgcgct
    36061 cggcgtttca tccgcaccat gtttctcgca ggtgctttac ggttcactgg tcggcaacgt
    36121 gatggacgag tcgcaagcgg cggttgccgc cgccgaggtg acaatcacga atcaggagac
    36181 caacctgacg cggcgtgctg tgacaaacga ggtcggcctg tacacatttc ccagcgttcc
    36241 gtcgggccaa tacacggtga aggtcgcgac gccggggttc ggagaatttg tacgtcgaga
    36301 tgtacccgtg gcagtgaata cgacagtacg tgtcgacgcg ctattgcgcg taagcggcgt
    36361 cgccgagcag gtcacggtta cggcgcagcc ggatgccgcg atgcttcaaa ccgaccgcgc
    36421 cgaagttcgt cacgaagtgt cacgaagaca gatcgagaac ctcccggtgc cggtcggtcg
    36481 cagctacgaa agtctacttc agctaatccc cggtttcgaa ggagggtcgg ctagagagga
    36541 gcgcctggga ggcaatcgcc ccgaacgatc gcagaatttc aatgtcagcg gcaccacaaa
    36601 ctcgacggtc gcgcgaaacc tcgacggcgc gagcaacatg cacgtatggt ccgtcggcgg
    36661 aagcgccttc gtgccgaatc tcgaatcgat tgaaactgtc gacgtcgtga cgaacagctt
    36721 tgacgccgag cagggacagg cgggaggagc ggtcattaac gtacaaatca agagcggcag
    36781 taatcaattc cacggctcgg ccttcgagta ccactacaat cagcacctga aagcgcgcga
    36841 ttacttccgt ccggcgggcg aggcgaaggg caagcagatc tcgaaccagt tcggtggcac
    36901 gctgggcggt cctatcaagc gcgataaagt gttcttcttc accagttggg agggtagagc
    36961 ggaacgggag aatctgggtc ggttgctttc gcttccatcg cgggccttgc gcgccggtga
    37021 cttttccgag ttcagcgcgg tcatctacga tcccatgacg gggaatgcca acggaacggg
    37081 gaggcttccg ttcgaaggca atcgcattcc agagcacagg atggacacta tagcacgcaa
    37141 ggtgataagc ctggttccgc tgggaaacct gcccggcatg caagacagca acagcaacaa
    37201 cttcttcgca tctgtgccgt tcaaagcgaa cagctaccgg accgacaaca aggtcaactg
    37261 gcaggcgcgg gacaacttca acctgttcgg cagcttgagc tactttaatt tcgacgtgat
    37321 caacccgacc gcctacggcg agtccattga cggtccgcgc gtcagtggag ggaacgccgg
    37381 gcactcatgg ggatacaacc ggcgcatcag caccggcgca aactacatct tcggtccgac
    37441 cttattgatg gacgctttct ttgggtggac gcgccagaac acgaatgtag agcaaccggg
    37501 aatcgagacg aactatggca gcgatgtgct cgggattccg gggacgaacg gccctaatcg
    37561 cttccaaggc ggctggccga tgttcagcgt tagcggtttc gccgtattcg gcacggaaga
    37621 agcgtacagc ccgtactacc gcaacgacga ccagttctca acccggtcga acttcacgaa
    37681 gacactgcgt tcgcacgaaa ttcggtgggg cgtggacatc aatgcggagc agatgaatca
    37741 tacccagccc gagtttcaag gcggcgccaa tacgggtccg cgcgggcggt ttaacttcga
    37801 cagcggaccg acacaactgt gcatggcggt ggcggcgaac ggcaactgtc aaaccctgag
    37861 caccgctgta aaccaagcta acgcctttgc ctctttcctg ctcggtctgc caactcaact
    37921 cggcaagaat ctcctcactg aggcacctta caacacgcgc acctggcgct acagcctgta
    37981 cgcgcgcgac cgctggcaaa cgaccccgaa gcttacgatt tcctatggtg tccggtggga
    38041 gtatttccca atgccgactc ggacggatcg gggcttcgaa cgatataacc ccgaggacaa
    38101 caagatgtat atcggcggcg tgggtaacgt tccgaaagac ttgggcgttc actcgagtaa
    38161 gaccttgttt gccccacggc tgggcgttgc ctaccgcgcc acggacacgt ttgtcgttcg
    38221 cggcggctac ggcatcaact tcgatcccta ctcgcttgca aaggcgctgc gcacgaatca
    38281 cccgatgctg attgagttgg tggttccatc tcgcaacagc ctctcgttcg ccgggcgtct
    38341 ctccgatgga atcccaccaa tcaacgcccc aaacctgggt aatggcatca tcgacgtgcc
    38401 tggcaacgtc tcgacacaga ccattccgct ggacctccag cgcggttaca ttcaatcctg
    38461 gaatctcact ctccagaagc gcctcggctg gggattcgtg ggtgaagccg gatacgtggc
    38521 gacgcggcag gtgcggcaac tcggttatcg ggagctaaac tggtctccgg ttggaggcgg
    38581 cacggcgggg caacaactga accggcgctt cgacagaact gcgaacacgc gcgaagtggg
    38641 cccgatcgga ggaagccatt acgactcact gcaggcccag ttgcagcgcc gcttcacggg
    38701 tggtgcgctg ctgcaggtag cgtacaccct ttcgaaatcc atttctaatt cgggcgtgga
    38761 cagcagcgcc ggggcgcctc cgatcatcat cccggacttc tatcacctaa accgctccgt
    38821 gagcagcttc gaccgccggc ataacctgca ggtcgcaaac gtgacgcccc ttccatttgg
    38881 gcggggcaag cactggctga accaaggagg aatactctct gcaatcgcag gcgggtggca
    38941 ggcgaacagc attgtcagtg tgatgagcgg acgtccgttc tcggttctcg cctctgcgtc
    39001 atcgctgaac tcgcccttca acagccagcg cgccgatctc atgaagacgg acgtcgagat
    39061 tctcggcgga gtgggacgtg ggcagccata cttcgatccg acggcgttcg ccgcggtgaa
    39121 tcaggcgcga ttcggcaccg ccggtttcaa cattcttcgc gccccaggcc gcgtgaattg
    39181 ggatctgggg ctgttccgta acatccggat tacggaggcg gtgaagatgg agttgcgcct
    39241 ggaatcgttc aacttcacca atactccgaa gtttggcgcc cctggcaaca atgtgagcaa
    39301 tctgcagttc aaccctgacg ggtcgattcg aaatctcaac ggcttctcgg agatcacgag
    39361 cgccttcgag gaacggcagt tcagccttgg cgtgcggctc agcttttgag gggagtgctg
    39421 gatgaataag gttcggatgt gcattgccgc gtctgtggcc gctattacgc ttgcggtcgc
    39481 gacagaagtt gcagtgccgc cagcttcgtc gcgagaactg gcgcggcgca agcaccacgt
    39541 ggcgaattca ccgcgaacgc cggccccgga acccggccag gagaacacga cgattctctc
    39601 accccttctg aagccgaacg gagaaaaggt gacgagtgcc gccgagtggt acagcacgcg
    39661 ccggcctgag ttgttgaagc tttggaccga agttctgggc aagaccgaac cgaacgaggc
    39721 cgaccgtaaa tggtttggcg acatcacgaa gacgataatc aagagcacgg aggaacagcc
    39781 gggttatacg cgcatcgacc tcgacattcc gctggaaacg gatttcctgc agcaccacct
    39841 cttgctcatc cccagagacc agggaagggg accatttccc gccgtgatcg cctgggccgc
    39901 gagttctgga gactaccggg acccggaacg ctggtggggc gagttcttgg cgtcaaatgg
    39961 atatgtcgtg ttgagcagtt ggtcgcttat gcgaaattac cgcaacgcga ccacgagccg
    40021 gacgggtgcg ccagacctgc tgtacgaccg cttcgggcgc tggctgccga tcgcgcggat
    40081 ggcgtacgac attcgccgcc aagcgcaatt ccttaagacc cggcccgaag tggacggctc
    40141 gcgtatcggg ttcatcggtt tttcgcacag cgccaaggca gcggtgtacg ccggcgcttt
    40201 cgctcctgag attcaggcgg tggtgtcagt cgatccgcac atcgcgatca acggcggtac
    40261 gaactactat gatccgtggt atctgagctg gcagcgtaag ttcaaagaca ttcggacgga
    40321 gaccggtacg gttcaaagcc tcctgaaccc ggatatcaag cgtcccggct tcgagcgcga
    40381 tcaccacgaa ttgttggcaa tggccgcacc gaggccgttt ctactgatcg gcggcgatca
    40441 atcggaaggc gatggcgggc actcggacga tctgcaaagt tggggctact tcaaccacgc
    40501 caaggaggtt tatgagcttc ttggcatcgc cgagaggctg cagttcgctt ccaccgccga
    40561 cggccacaag gcgaacgggc ccaacatcga tccggcttgg aagacttttt ttcagcgctg
    40621 gctgaaagag accccagtcc gcttcacagg ctggcgggac cagtacggcc ctgctcgcaa
    40681 ccgttaattg tgcgcatccg cttcggtatg agcccggctt tcttcgcctg cgacacacct
    40741 tttgccatgc cggaacgagg agggcgcctc acgggcggct tattcgctgc gcgacttcag
    40801 ggaggccaat acttcccggg tctcagagat ctgcctgagc aggctcttct tatgccgcgt
    40861 agcttgatgg tccaggcgct ctccgggaga aaccaattcc ttctcgagct catccagatg
    40921 ctgttcgcac tctgaaatgc gctgccggaa cttgccccga ttctcaaatc caccgccgtc
    40981 gacgctgacg tagacgggag tggtatgagc cagttgagtt gggcctgctt tgacgcgtgc
    41041 ggcaatccac aacccgtggt ccgctgccat ttccatgtca atggagagtt gtgcggcgct
    41101 ttggcctggc tgcgccgcgc gtgctgctct gagcacttct ccgtggccga cgatctcaag
    41161 ctcttcgagc gggatctgtt tcgattcgcc gaacgcggtg gctttaattt tcagaacttc
    41221 cccgcggcgt acgttcacag tatctccagg caaccggcca tttacggtaa gctgaacgat
    41281 cggcccgctg ctcgcgaacg tgtgtcccgc cttcactgcc ttgagccatg aagcaaacgt
    41341 gaactcgttg ccaacgtacg tgtagaatcg ggagtcgcca atttgcgatc caccctcttt
    41401 cagaccatat cgaggcccgc ggccgcacca gggaaagtcg gagccggcga gcgctgtcag
    41461 gcgatagcct aaatccagaa agcggtaata gtgatcgacg accagcggac cttcggcagc
    41521 gcaaaactgc attagctcca ggaagtcgat ttttccgcgc aggacgttga gggccattcc
    41581 gcgatagcca tgaaatgtca ttgcctgatg cgcgaaacca gtgatgccat cgagctcgtg
    41641 cacccggtcg aacaccctgt cgtacgaata gtagtcgggc tggaagcgga caaagtcctc
    41701 cgcgcccagc gatatggtgt ggccgatctc gggcgtgcgg ggctcctcct gaccgggcga
    41761 cagaatgttg tcccgttccc ggtagcggcc tttctcgccc caggcatatt gcgaataata
    41821 ggtggcccag aaatcgccca tctgtaacag gtgcccgacg tgaatatcct ccgcggatat
    41881 ccaccgcacg atggaagcgt cgtcgcggga cgaccggcgc aagtgaatat gatcgtcgga
    41941 tgaataccac ccgcgttccg gcatgttgat ccaatgttcc atccggtacg tagtccgggt
    42001 ccgcttaccg gctgagatac tcaatttgtg agtttgttga atatactcat aacccttgga
    42061 gaggctgagt gtgtagttgc ccgccggaag ttttacgtca aacgatccgt ccacgtaaaa
    42121 ggcgccgtcc ggttgcaaca gaaagccttc ggctctgtcg gactgcccat acattactcc
    42181 aatggcagcc gccggaacac cgaaagcagc atcggagact gccagcgccc cggtgatttt
    42241 gggaggattc ccgttggaat cgagaagcct cacccggaca ggcgtgggtt tccccgttcg
    42301 cgcatctacg attcggaccg aaatggaggc atcgccagta gcaaggaccg catagcagat
    42361 tgccatgacg aaaagcgcta tgaggagaac acgggtggga cgcatgctca cattctctct
    42421 gaaatgaatg aatggtgggc gcgcagggac tcgaaccccg gacctcttcc gtgtgaagga
    42481 agcgctctaa ccaactgagc tacgcgcccg gcgggcggga actctcattc tagaacgatt
    42541 ggcgcgaccg ccgccacctg ctaaactatc ttctgggaca cggcggcttt agctcagccg
    42601 gtagagcgct ggattgtggt tccagatgtc acgggttcga atcccgtaag ccgccccaaa
    42661 ctccaaggct acttccgccc gggaggatcg acagccgtgt caaggcggag ttctttccaa
    42721 agccgggctt tcatgtcgcc cagccagcgg tcctgatacc ggcggttcag gtcgatcgtg
    42781 gccgatgcga ctcctggaag cgtcgatgcc ttggatagca tttcgccatc cgggtccatg
    42841 atgtaagtcg gatgatcgta gcctgacgcg atgagataaa cctggttctc aatcgctcgt
    42901 gccttggcca gtgtttcgtt gccgccccaa atgggcagca gaatcatttc cgctccgcgc
    42961 agggcgagtg cgcgcgccgg atcggcgtac tgaacatccc agcagatcat cattccaagg
    43021 gtgccaaagt ccgtctgaaa aaccgcataa tccgaaccgg gcgtgattcc gccttccgtc
    43081 tcttcccggg gcagatacac ctttcgatac ttcccggcga gttgcccctg gcggtcgatc
    43141 aacaccgccg tgttatagat cgtggtgtct tcgcgctcat acaaccccgc cacaatatag
    43201 gagttcttcc ggcgcgccag ctcgcccagc gctttggtgg tgggacccgg caaaggttcg
    43261 gccacatccg cgtatttcaa cccggttccc acgaccgtaa tgccctcggg taaaacgata
    43321 aggtccgctt ccccgaccgc tttctcaaca tagtcgagga aactgtgcac tgccgccgca
    43381 gacgagggtt gctgtttggg gcgatggttg atagaggcga tgcgcacgtt cctggggccc
    43441 ggcttttcaa tttgcgtgag cgatacatcg tcccagtaga gcgtcgcatt aggagcattc
    43501 agcaggaaga gctggatggc ggcgccagat gctttctccg gtgcgggtgc ttcgagcgtg
    43561 atgcgattcc actccccatc tcgcgtgacg cggtaaccgt agtcgggctg cccggtccgc
    43621 cggcccgcgg aactacgcca atcgagacgc gcccacacct gtaagggctc gtaatccagg
    43681 ccctccgagc gataatgcgc caccagccga taccacttgc cgggttcgat tcctttcgca
    43741 tcgtattccc aaccgccgaa tacaccaacc ctgctattac cggaaacagc caacgagccc
    43801 ggccgttttc ggaagtgaac ggcgtcgacg aatgtgcgcg gagcgatctc agcgcgaggg
    43861 gaccaggatt tccagcccgc aggcgagcca tccgcgcttg tctgaaaacc ggtttgttca
    43921 aacaggggcg gcgcagctac ggctgccgag aaaaacacga agagcgcagt aattgcacgc
    43981 atgtatctat tgtgtcccaa tcgcggatgg tctcgctccc ggcacgccac cgcagaattc
    44041 gataaaacgc gtctcggttg cccgcagcct ggtagcaatg agttccgcca cacgccggaa
    44101 tatcgtagag cctatggccg ggtccttctc acaggcctcc agtaacaagg gaccgttgag
    44161 gcgcagcgcc tcacaccgtt ggcgcgcccg cacctggaaa gcggcatagc gctgctccac
    44221 cacggaggac cagccgaacg cttctccccc gcccaacacc tggatgcata ccgcgtatac
    44281 cggcgtttgc agttcaagaa acgccgttcc tgaaaggagc aggtataaat agtcagagcg
    44341 ttcaccgccg cggaaaaccg tctgttccgc ttcgaaggaa accggttcag ccaaacctgc
    44401 aagaacttcg agttgatcgg cggtcaatcc ttgtgtaaaa ggatgggatt cgagtgagtc
    44461 cagtatgtga tgatcggtca tgttaggtgg aagatattct atttcggaaa ccggccggcc
    44521 agtgtccttt gctcgccggc ctcctttcgt ttaccaagct tttccagtga gtccgcccag
    44581 gcgagccgta ttgccggttc gtccgggcgc aactcgaccg cgcgccgcag actgttctcg
    44641 gcttcccgcg gatctccggc cgccagcgaa ttaataccga gctggtacca ggcctccaca
    44701 aattcggggc ggctctgcaa cacaatttcg agaatcgcgc gcgcatcctg gaagcgccgg
    44761 agcttggaat atgccaatgc cagttggtag cgcgccagtg agtccggttc gccacccagc
    44821 agccgcacca cttcctccgc ttcgcctttt ttcaacagcg caacagccag gtccacggcg
    44881 atggaagaat ctcttaccag cgcgagcgcg gcgcggtact ccttgatcgc cccgtccaga
    44941 tcaccggctt gcgctttctg atgagcgacc gccgcccggt atttcgcctg catccgcgca
    45001 tcggcgccgg tgcggaggtc tttcactcgc gccattgccg ccgcggcctc cgcctccctg
    45061 cccatcttct tcaaacaccc ggccaggaca tagtatttct gcggaacttc ggcgccggct
    45121 tcgagtagcg gaaccgcgcc ttcgcaatcg tccttttttg ccagggcggc gcccagttgg
    45181 aagctggccg cgccgttgcc gggctccacg gcaagaacgt cacgcagcag gataatcgcc
    45241 cggccagtct cctgcgtgtc gagataggcc atcgccagtt cgaacttcac cttcggaaat
    45301 ttcggattca ggcggagaac cgtctcgtac tcctggaccg ccgcagccag gttgccgagc
    45361 tttgcttcgt tccaaccagc gtggaagtgc ggctcaggat cgctcgcatc gagtgcactc
    45421 gcggcacggc aggaatctct cgcttcatcg tagccgctgt ccttggcacg cgcgaaaacg
    45481 gtgagaagtt cgcagactcg cgcctgcgcc tcggcgtatc gcgggttgac gtcaagcgcc
    45541 gaccggaaag attgaagcgc cgcggcggcg cgtccctgcg tctggcgaac cagacctaat
    45601 agataatgcg cttcagcgag tttcggatcc atcgcgagcg cacgcacgaa tttctcagcc
    45661 gcttcgttga agcgtccctg atgcgagagc tcgatgccgg cttctacgag cgccggtgcc
    45721 tgcgcggacg gcgcttgcag aaaccccgca agcagcgcga cgaaatacag cacacgtcta
    45781 aactaacatg caccgcccta ccatgtgagc cgtatggtta attggagcac gcgcggcgcg
    45841 ttgtccgaag tgcggttcag cacaattcca tccccaccgg gcacagtggt gccgggcatg
    45901 ttgaatacgt tctccagaaa atcggcattc aggcgcagga acacacgttc cgtaatgggc
    45961 acggttttga aagccgacgc agccatattc catcttatgg gcgccgtgaa atactggttc
    46021 tgaagccgat gcatgcccgt gtcataggtg gtccgctggt tggttccatt cttcaacgga
    46081 acgaagacgg tattcgattc atagaatgcg aagttcggat cgcttgaact tcccccattc
    46141 gcgggtgtcg gaatcaacca cgtctggaat ggccggtaat tctcgggaac gcccattacg
    46201 ccgttcggcc ttcctgcgga atcgacgctg ttgatgcggt tcgccggaat gtagccattc
    46261 cagtacagcc agccgtcgta acagacaccg ctccggcaat ctgccacccg ccgatgagcg
    46321 cgttcacaaa cc
//
LOCUS       DASTMK010000345         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_400664, whole genome shotgun sequence.
ACCESSION   DASTMK010000345 DASTMK010000000
VERSION     DASTMK010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400664"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFQ79_15130"
     CDS             complement(<1..348)
                     /locus_tag="VFQ79_15130"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0141054.1"
                     /translation="MARKKQSDQLTPLELEIMKVLWDAGSATVQTVQERLPIEPRLAY
                     TTVQTMLNLLKKKGKVHRVHKPGERAFLYAPAVSRHSAVGQLVGDLVDRLFGGSAEGL
                     VMSMVEQRYLKPED"
     gene            complement(696..851)
                     /locus_tag="VFQ79_15135"
     CDS             complement(696..851)
                     /locus_tag="VFQ79_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141055.1"
                     /translation="MTAGAPAVEEPLRKGTTVGRYDVAAPLGRGGMGEVYIGWEESPA
                     FSRMANR"
     gene            complement(848..1012)
                     /locus_tag="VFQ79_15140"
     CDS             complement(848..1012)
                     /locus_tag="VFQ79_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141056.1"
                     /translation="MTIDKWREVLSCYQAALETPSDQRRELLESKSVDPEVIEKVLRM
                     LEALDRGKVK"
     gene            complement(1116..4076)
                     /locus_tag="VFQ79_15145"
     CDS             complement(1116..4076)
                     /locus_tag="VFQ79_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141057.1"
                     /translation="MKLRIAAATVFLAWSWQTAFGQVMVWDKMPGRVIDISGNVRAPR
                     IGEPATAGGLWALGIERVNASGFKVFRFSGGDWVRDYNAPSGRRLAAGPDGDLFILDD
                     QRNLHRYAGGSAQIIGQANDVAVGADGSVWIISTLEVLGGFRVSRWNGSAWMHTNGGA
                     VRIAVDREGSPWVVNDAGKIFRYNTASNQWELKRGTARSVHTGARSGAVWMLGADPIP
                     GGFPIYQWNAAKQDWDPYGSYGAVAITEWAAEYIVQSDGTIYKATYVLTQFTTIDITN
                     TVMPLMTPQAETPVRVSGDGKLLCSAADSGSATYCGNTNADYVGGHTLNLDCKEGFYD
                     LIYGGTCWKCPDDTDGKGAWIRSGDAVDKDTACWRVPNRPESTAHAIKVKSPAPASQC
                     PSGSFWDGYSPDGIGGSCWKCPDDHPRRTAAAVWAGNACATSLNETRPATLLSFNGCP
                     TPDAAKMGLAGKRSPGKPFLDIAARGCFACPITDEEGNFLLTDRNGNPLYNRTSNDGC
                     RVNMKWQPFPFREPGLAYMQGVKQVIWDLRLFDGGKLTGFLYDSAEAKGLGDATPGAK
                     AWVSERWAEIAAKPYNSESFRAFMFAVLKTAARKNVKDRTPGEQKLIESFQDYIRDRR
                     TYLAEQGLLMYDAWKAYNDAYRQETGQTKSFSQLFYYGTVPFDYHAMVGSLMGLGGAG
                     GGVAGAIVAINQFASNTLYLQTVFEGGNAYWRAQETVGFLTSGLNLLKSVQGVTLLSG
                     ASVISIVGVILTSIAMDQFMAIETARPKLEASLAEAMKPVDLSSFTKVKNGEDMLYFF
                     WAKAMDAVDAEDPQVRQLASQARARADQSGYQAPPKQLIKVTLESISLGPAVSSGTGT
                     ASPFSSPGLAQNEMLVSNNGKYEARMQLDGNFVIYSQDTPIWATQTHGQERAPYRLAM
                     QPDGNLVVYGSRTSDVALSGYGTCKANSPCIAIWNTGPRGGTAPYSLEMQDDGNLVMY
                     DNTHRPVWASGTNR"
     gene            complement(4140..>4289)
                     /locus_tag="VFQ79_15150"
     CDS             complement(4140..>4289)
                     /locus_tag="VFQ79_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0141058.1"
                     /translation="HSFATHLVEGGADLPTVQAIPGHADLKPTSIYLHMSERHLRAAG
                     TPIKV"
BASE COUNT          850 a         1326 c         1213 g          900 t
ORIGIN      
        1 atcttccggc ttcaaatagc gttgctcaac catgctcatc accaagcctt ccgcggaccc
       61 gccgaacagc cggtccacca ggtcgcccac cagttgtccc acggcgctat ggcggctgac
      121 cgccggagca tacaaaaacg cacgctcgcc cggcttgtga acacggtgca ctttaccctt
      181 tttctttaga aggttcagca tggtctgcac tgttgtatag gccagccggg gttcgatagg
      241 caaacgctcc tgaaccgtct ggacggttgc agacccggcg tcccaaagca ccttcatgat
      301 ctcgagttcg agaggcgtca actggtcaga ttgtttcttg cgagccattt tcctaaacaa
      361 ttaggagtat aagcccagcc cgccccgcgt gtcaacgaat aaattaggac tcagtaagac
      421 atcaaaacgc cgggtgcgga tgtgacttaa accataactt tgacgtttga gaaggatatg
      481 tttatttgat tttatttcgg ccttcgattc ccaaatgaac gatgccgtgg aacccatcct
      541 tccgcaatcg acagtaacgc gggcggtaaa agggagcgtt cccactaaac ccttggcgac
      601 caggcaggag taatctgctg aggagccggt aattagaggg cttcgccctt tgcagatctg
      661 ctggcggtga ttcggcttcg gcatccatgt gaaggctagc gattcgccat ccgggagaaa
      721 gcaggcgatt cctcccaacc gatgtacacc tcgcccattc cgccccggcc cagcggcgcg
      781 gccacgtcgt accggccgac cgtcgtcccc ttccgcaatg gctcctcgac agccggggca
      841 ccggccgtca cttcactttt ccccgatcga gcgcctcgag catccgcagg accttctcga
      901 tcacctcggg atccactgac ttcgactcga gaagctcgcg cctttggtcc gaaggcgtct
      961 cgagggcagc ctgatagcag ctcaagacct cgcgccattt atcgatggtc acgactggtg
     1021 cgcattatgc cgaaagcaaa tgagctgcag gaggggaacg gaagatggaa aacgagggtg
     1081 cggcggcccg ggcgggccgc cgcaactgaa gaacgtcagc gattcgtgcc gctggcccaa
     1141 accggccggt gcgtattgtc gtacatcacg agattgccgt cgtcctgcat ttcgagactg
     1201 tacggcgcag tgccgccgcg cgggccggtg ttccaaattg cgatgcacgg ggaattcgct
     1261 ttacaggtcc cgtagccgga aagggctaca tccgaggtcc tggagccata caccaccagg
     1321 ttgccgtccg gctgcatcgc cagccggtag ggtgcacgct cttgcccatg ggtttgggtg
     1381 gcccagattg gcgtgtcctg ggagtagatg acgaagttgc cgtcgagctg cattctggcc
     1441 tcgtatttcc cgttattgga gaccagcatc tcattctgcg ccaggccagg gctgctgaac
     1501 ggagaagcgg taccggttcc cgaagaaaca gcgggcccga gtgagatcga ttccagggtg
     1561 accttgatga gttgtttcgg aggcgcctga tatccgctct gatcggcccg tgcccgtgcc
     1621 tggctggcca attgtcttac ctgcgggtcc tcggcgtcca cggcgtccat ggccttcgcc
     1681 cagaaaaagt agagcatgtc ctctccgttc ttgaccttcg tgaacgagct caggtccaca
     1741 ggtttcatgg cttctgcgag gctggcctcg agtttggggc gcgcggtttc gatagccatg
     1801 aactgatcca ttgcgatcga ggtcaggatg acaccgacaa tcgagataac ggaggcgccc
     1861 gaaaggagtg tcaccccctg caccgacttc agcaggttca gtcctgaagt caaaaacccg
     1921 acagtttctt gcgcccgcca gtaggcattg ccgccctcaa aaacagtctg gaggtacaaa
     1981 gtgttggagg cgaattggtt gattgcgact atggctccgg caacgccgcc gccggcgccg
     2041 cccaacccca tcagcgaacc aaccatcgcg tgatagtcga acggcaccgt cccgtagtag
     2101 aacagttggc taaacgattt tgtctgtccg gtctcctgcc tgtatgcgtc gttgtaagcc
     2161 ttccaggcgt catacatcag cagcccttgc tcggccaggt acgtgcgccg gtccctaata
     2221 taatcctgga aggactcgat cagcttctgc tcgcccggcg ttctgtcctt gacgtttttc
     2281 ctggctgcgg tcttcagtac agcaaacatg aaggcccgga aggactcgct gttgtaaggc
     2341 ttggcagcga tctccgccca gcgttcagac acccacgcct tggcccccgg tgtggcgtcg
     2401 ccaaggcctt tcgcctcggc cgaatcgtag agaaacccag tgagcttgcc gccgtcaaac
     2461 aatcgcagat cccagatcac ctgcttcacc ccctgcatgt aggcgagacc gggttcacgg
     2521 aagggaaaag gctgccactt catgttgacg cggcaaccat cattgctggt tctgttgtac
     2581 agcggattgc cgttacgatc ggtgagcaga aaattgcctt cttcgtcggt aatggggcag
     2641 gcaaaacagc ctctggcggc gatgtcgaga aacggcttcc cggggcttcg tttaccagcc
     2701 agtcccatct tcgcggcatc gggtgtagga cagccgttga acgacagaag cgtagccggc
     2761 cgcgtctcat tgaggctcgt agcgcaagca ttaccggccc agacggcagc cgccgtcctg
     2821 cgcggatggt catcgggaca tttccagcaa cttcccccga tgccgtccgg cgagtatccg
     2881 tcccaaaacg atccgctcgg acactgcgag gccggcgccg gcgatttcac cttgatcgcg
     2941 tgagccgtgc tctctggccg gttcggaact cgccagcagg cggtgtcttt gtctaccgca
     3001 tcgccggacc gaatccaggc gcccttgccg tcagtgtcat cggggcattt ccagcaggtg
     3061 ccgccgtaga tcaagtcata aaaaccctct ttgcagtcca aattgagcgt gtgtccccca
     3121 acataatcag cgttcgtgtt gccacagtag gttgccgagc cgctgtcggc agccgagcaa
     3181 agcagcttgc catcccctga cactcgcacc ggagtctccg cttgcggcgt catcagcggc
     3241 atcaccgtat ttgtgatgtc gatcgttgta aattgcgtga ggacgtacgt cgccttgtag
     3301 atggttccgt cggactgtac aatgtattcc gccgcccact ccgtgatcgc cacagcgccg
     3361 taggacccgt aggggtccca gtcttgcttt gcggcattcc actgatagat cgggaagcca
     3421 ccggggatgg ggtcggcccc taacatccac accgcaccgc tacgggcacc ggtgtgaacg
     3481 gagcgtgccg tcccccgctt cagttcccac tggttcgaag ctgtgttgta gcggaatatc
     3541 ttgcctgcgt cgttcacgac ccatggagaa ccttcacgat ccaccgcgat acgcaccgcg
     3601 cctccgttgg tgtgcatcca cgcggaaccg ttccatctgg agactctgaa ccctccgagg
     3661 acctcgagag tgctgatgat ccagacggag ccatcggcgc caacagcaac atcgttcgcc
     3721 tggccaatga tctgcgcgga gcctcccgca tagcggtgaa ggtttctctg atcgtccagg
     3781 ataaaaagat cgccgtccgg accggctgct agacggcgac cggagggggc attgtaatcc
     3841 ctgacccaat cgccgccgct gaaccggaat accttaaatc ccgatgcatt cacccgctcg
     3901 atcccgagcg cccacagccc gccagctgtc gccggttcgc caattcgcgg tgctcggacg
     3961 ttgccgctga tgtcaatcac gcggccgggc atcttgtccc aaaccataac ctggccgaat
     4021 gccgtttgcc atgaccacgc cagaaagacc gtggccgctg cgatacgaag tttcatgccc
     4081 ccccctcatc ttgaataggg cgagcacacg gctggtttcg atcatgtgcg ttggcggctt
     4141 caaactttga tcggtgtgcc cgctgccctc agatgccgct ccgacatatg gagataaatg
     4201 gaagtgggct tcaggtcggc atggcccgga atcgcctgga ctgttggcag atcagctccg
     4261 ccttccacca gatgtgtggc aaaactgtg
//
LOCUS       DASTMK010000346        15589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_113168, whole genome shotgun sequence.
ACCESSION   DASTMK010000346 DASTMK010000000
VERSION     DASTMK010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15589
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113168"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..494)
                     /locus_tag="VFQ79_15155"
     CDS             complement(306..494)
                     /locus_tag="VFQ79_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141059.1"
                     /translation="MRRAKRQHGLTLSATRIRLPTSARYFRYFPTEAFARPAEFSFGD
                     AGKLVIRGRCTFDLALSR"
     gene            complement(639..2978)
                     /locus_tag="VFQ79_15160"
     CDS             complement(639..2978)
                     /locus_tag="VFQ79_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0141060.1"
                     /translation="MRIEHAMSAFAMSSMTQQIKVLLVEDNPADARLFRETIVDTRRL
                     PPLLTHVQRLGEAIERLRAERFDVVLLDLSLPDSEGLDSIVRVHEKVPEIPIVILTGH
                     DDEQLALKAVRAGAQDYLVKGQGDGNLLLRAMRYAIERKRIEEALQQREEHFRSLIEN
                     ALDVITILSKEGVIRYESPAVARVLGYEPENLEGQRIAPYVHPDDTPQLNRLLGGAGR
                     SDALARSVEFRMRHRDGSWRTLEAIGKDFIDESGQPGVIVNSRDITSRRRAEEGLREA
                     HATLRTVIETAPVAIYTLDCNRHVLSWNACAEKIFGWTAAEIVGKPLPTIPPALGPES
                     MEQFERLCHGETLAAYETSRLRKDGTLIDVTIWTAPLRDGKGNIYGIMSTVADVTERK
                     RLEDQLRQSQRMDAVGQLAGGIAHDFNNILTIIAGYSDLVLGVLDDKAPLRRNIEEIK
                     KASDRAAALTGQLLAFSRRQVLQARVLNLNAIILDVETMLDRLIGEDIELRMDLDQSL
                     GNIKADAAQIEQVILNLIINAREAMPGGGRLVIGTRSITIKDETGEPGLALASGRYAV
                     LSVADTGHGMTAETASHVFEPFFTTKPKGKGTGLGLSTAYGIVRQSGGEIAVRSRVGE
                     GSTFDVYLPIVDEAATIPASSAIVAAPARGTETILLAEDEDTVRELVREVLQNQGYTV
                     LAASHGAEALELALNRGERIDLLVTDLVMPHVNGRDLAERSTAAHPEMKVLYMSGYTD
                     NTLVPGGMLTPGMEFIQKPFSPEDLARRIRQVLDSRKNS"
     gene            complement(2982..4820)
                     /locus_tag="VFQ79_15165"
     CDS             complement(2982..4820)
                     /locus_tag="VFQ79_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0141061.1"
                     /translation="MNLVRWIAGLAVASELIAAVPVLHVRVPMRDGVTLCTNVFLPST
                     SSRFPAILVRTPYGKGSQPGTYSFLASRGYAIVLQDVRGRYHSGGTFRSMDQEGPDGD
                     DTIKWIARQKWCNGNIGMAGASYAGMAQWKAAEMNNPNLKAIFPIFAGSDEYLDRYYS
                     PGGALKLGHRLLWMAGSFRARGFASPGFDRYVLHLPLRTADRAATGQRLAFFQSALDH
                     PTYDAYWRTIRSRDSLSRIRTPAFSIGGWYDNYVQGDLEAFSVLGKTGRHWLVVGPWA
                     HDMSAKFEATDFGLQSALALRKLQLEWFEYWLKPHPRGVKSAFPQPPLRMFVMGANQW
                     RDEREWPLERSHEVPFYLSSGGHANSIEGDGVLADRPDWHQAFDSYVYDPLKPVPTRG
                     GAVCCNPKVFPWGPMDQRPVEARHDVLVYTTPELDEDVEATGPVRVVLFVSTSAPDTD
                     FTAKLVDAGPDGYARNLTDGILRLRYRKSLERPVSAIPGEVYQISIDAGVTGHVFKTR
                     HRIQLEISSSNFPRFDRNLNTGRRAADDTQIHTAVQRIYHGRRYPSRLILPVALAGAE
                     RANPGAASSSNSLCYTEVRLFRLVSRLPQITLFGRGPGTGTDRHKN"
     gene            complement(4896..7259)
                     /locus_tag="VFQ79_15170"
     CDS             complement(4896..7259)
                     /locus_tag="VFQ79_15170"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEU0141062.1"
                     /translation="MKKKVVAPVSVAIGAALLYLVYPEAAVSAQGHLTQGALFRIDPR
                     GEMTSACPLKHTDVKAEISGFIARVTVTQQFENPSEEKIEAVYTFPLPDKAAVDDMTI
                     KAGDRVIKAKIKRREEARAIYEAARAKGNVAGLLDQERPNIFTQSVANIAPGAKVTVQ
                     ISYVETLKFDEGAYEFSFPMVVGPRYIPRQVTDAPRIAPPVAAKGERAGHDISLEVKL
                     DAGVPIEKLRSVTHDVAIDAPSSTTAVIRLKDKVVIPNKDFVLHYDVSGAKIQDAVLA
                     HNTGQDGYFTLMLTPPERFTVEDVTPKELVFVLDTSGSMSGFPIEKAKETMKLALEGL
                     YPRDTFNLITFSGDTHVLFPAPVPATRENLDKAQQFLAARQGLGGTEMMKAIIAALEP
                     SGKQDHIRIVCFMTDGYVGNDFEIISEIQKHKNARIFAFGIGNSVNRFLLDNMAREGR
                     GEVEYVGLNDDGSAAARRLHERMRNPLLTDIRIDFGGVEVSDVYPQRIPDLFSAKPVI
                     LTGRYARAGSGTVRLRGTMSGKPFERTMTVSLPASEPRHNVLRSLWARQRIGDLMSRD
                     YAGLQRGNVEPQLKEQITQLGLDYRLVTQFTSFVAVEETTVIEGGEPRRVEVPVEMPE
                     GVSHEGIYGERVAVAGLRSTGFAGYAGMQATGGFARGGVVGGVIGGVPSPAPMPSPRR
                     VPAEKRRDSQEQLAISRLHPDVAAAISQLKKGATPSAAFIRDGKAYLQVWLSDASPEL
                     LKQIRALGFETTVDPRVAKLLVGRIAVGKLPALAAMREVRYIAPQQP"
     gene            complement(7319..8575)
                     /locus_tag="VFQ79_15175"
     CDS             complement(7319..8575)
                     /locus_tag="VFQ79_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141063.1"
                     /translation="MSSGEARKLLGGYSAGTLTPEERKALFEAALEDSELFELLMEEQ
                     PLLELLTDPAMRTELLGALDSHEKGWFARFWNNVLKPAPVALAGSVAVAALAFFVVYR
                     FDHTEIRQPELVAQSHRPTLPEAAPAEAPAPEPPAPKQPATPSAGMRRKAEILPERAA
                     PPRPPALPPPPAIPVERELRSASKTVSPMFAERDLSSVPPPPGKSELSRATRLGEAPV
                     GVVGGVVGGVPGALGGYAAEPQAPAAPSSASAWFYGRAISAVQPMAGFRQPAPTAAVT
                     RRADTPARLGLRYSIVPSQQRARLQVQSNEPAFLQVLGRSIAGPWRLVHQGDIKKWDS
                     LLIPAPLTGQSHILLILTRKRDPGFAIEPDSPAQAGSLAIRALEAGAAAPPLVEQRGA
                     STYVIAPESHPDRLIKEFDVTGAKQQ"
     gene            complement(8572..9078)
                     /locus_tag="VFQ79_15180"
     CDS             complement(8572..9078)
                     /locus_tag="VFQ79_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0141064.1"
                     /translation="MERDEILARLRERIVAFAASRVMRDGAEDVAHEVLLLLEEKYPH
                     VTALEELVPLSMRIARFKITALYRKARRRGEDSAVSVDEIPLPDFAPDPEILASRREL
                     LDRIRAALPQVGQRCRELLRLKLQGRSFSEIRKAMGAHSLNTVYTWDYRCRQHLLELI
                     GGSWETKK"
     gene            9231..12416
                     /locus_tag="VFQ79_15185"
     CDS             9231..12416
                     /locus_tag="VFQ79_15185"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141065.1"
                     /translation="MKRTILAIFLFAAMAVAQTYYGTIRGTVMDATGAVAPGIEVILT
                     NTQTNIETRTVSNEVGNYVAPNLIPGSYRVSVEAQGFKKFVVEGVELVATADRRVDVR
                     LEVGSVAESIEVLGGAQLIETERATISDVKSNLVFTTMPVVSNYRSIWRMLALTPGVT
                     GDGSYYAGNGQGRNTTYSIDGIPIKDGWTGSSFGPALTYLDSYREFRVDMVSVNASGG
                     TSSNVAVVSESGTNALHGEAWLHYNAIGFGARPFFSPRASSGPPIYRPNVKAGGPVVL
                     PKLYDGKDRTFWHFSWQGIRGSQVPAVTNIVVPTAAFRAGDFSSLTTALRDPLTNQPF
                     ADNRIPESRISGVSKYYQERFYPLPNSGADRYTDVNVSPNTSDQYTARIDHKLTDAHS
                     FFGRFMYQHYAFERWDGENNPLIGNYEQWRDQYHFVLSDTYVLSPSTINEFRVGYARD
                     ASIYGGKNRGLDIVRESGLQLADLQDSAGLPRMNITGFSSLYQGNESGWTWSNYYVQE
                     TLHKIIGKHNLRFGVDIGDYNGRMITTSPSAVYGIYGFNGRFSGNPYADFLLGIMDTS
                     SRQTSVGPVYPHRSTWEFFVTDDYKITPRLSLNLGLRYSLLTPGTIEQDLLANFIPSA
                     NALVVVDDEVKARIHPGFPSNVPIATASSLGLDRSLLKLDRNNFAPRFGFAWRPGRWE
                     DFVIRGGAGVYYVAMQPYVSDGGGSPFELSESFTNSVTNGVPAFQFPRPFPATGYVLG
                     GTSANGMDPNLRTPYSTQYNLTVEKQVFDMGLSATYMSTLARKKVWTRNLNQVPADTR
                     PYSEKLLSVPFPYLFSANETINGSFHNYHAGTIKAERKFKDGFYYQAHLTWAKSMADD
                     WSGTEDVFNRARERSQGGQIPRWRGVVVGIYEMPFGHGKKFGSSMPRALNYIAGNWTM
                     AGTYVWQTGRYFSPAFDGVDPSNTNRFSGRPDRVADGNLSSDQRTLGQWFDTSAFVVP
                     AADIGRFGNSGAFILQGPGLSVFHFGVNKEIVLHERARLKLEMASTNFLNHPNFANPS
                     ATIGTSAYGQVLGTIGTDGNRDFQLTARLTF"
     gene            12545..13651
                     /locus_tag="VFQ79_15190"
     CDS             12545..13651
                     /locus_tag="VFQ79_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0141066.1"
                     /translation="MCTLSRRAFFAAAAGFSAAISAPGAPVPVAQYRRGGMIYRRLGQ
                     TSLYPSVLSFGSHTDPAYKRKVKHGSVLTEEGQQRRDRMLARALDRGVNMVDTYETEG
                     QWEHVARVIQSRRQSTLVSLCRQLPMFVGENIDRAARLYGHVDMYRIYINDGAALDAR
                     GIADWDVLRKAKEAGKVRAIGIATHDERLMVAALRELEGIDYIMFPYNFIHARADYTE
                     FLPAAIASGVGLIAIKPLAAGSIVKLDTRVVKGSRPESDVVQLYQKKYRPLHPAVVSE
                     LTRSLARLPDETLCQAAMRFVYSRPFLTCAMPGMFEEHTLEENYAGLTRHLELSREER
                     AALDAAAHFARATSGNWLTPGYRWLDTRWSPTRA"
     gene            13615..14949
                     /locus_tag="VFQ79_15195"
     CDS             13615..14949
                     /locus_tag="VFQ79_15195"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0141067.1"
                     /translation="MARYPLVPHARLTPLLLLAALAAAAQTSTNPERFQQAGVCSRCH
                     VAQVLEWSISGHPRAATGCEECHGPSKAHVVDERNQVKPDKMPLGAAIAGLCQSCHTQ
                     GCPKTERRASCESCHDPHALFHPKATNRPAPGSEDDVLPRFESHLKQGESLIEQRNWN
                     AAHKELQAALRLYPDHPRASARLALVNRRLNPSLPGFDILDDQFDAETGLPLHVRVSG
                     MPVEMVLIPGGETDLGNDAQPSARPVHTVSIAPFYLARTELTQGVWMTLDPANPSVHR
                     GDDLPINNISWEDAQRWIAKLNGRIAGGGFRLPSEAEWESAARAASKDIDSGERAWYR
                     ENSMAAAGSGEFRELDAYAPRRVAAKRSDGRGIHDLAGNVWEWCSTLMKPYPYDPRDG
                     RESPDTPGLRILRGGGFADSADYLAPWFRHAERRDRRLPFNGMRLARSVPAQ"
     gene            complement(14959..>15589)
                     /locus_tag="VFQ79_15200"
     CDS             complement(14959..>15589)
                     /locus_tag="VFQ79_15200"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0141068.1"
                     /translation="DTMRAYPGVFSGVGIVDSSSPHPEKTMRDLAKHGVRGFRITPGA
                     SPQSWLDTPGMEAMWRCGAEQNLAMCPLVGPDALPSIDRMCIRHPATPVVIDHMARIG
                     ATGEIRDADVRLLCGLSKHPKVAVKVSAFYALGRKTPPYADLAPLIRRVFEDYGPRRL
                     MWASDCPFQVQKEHTIAASIALIREGLPFLSAEDKEWLLTRTAESIFFG"
BASE COUNT         3013 a         4789 c         4696 g         3091 t
ORIGIN      
        1 ccctattccc gaatgaggct gcgctgttgc gcctggtcag cgccgtactg atggaaatca
       61 gcgaggagtg ggaaaccgaa aagatctact tacgaatgga aaacagtggc ccgattacgc
      121 gggaagactg aatttacaga aaaagcttga ctttgccaag cgtataggga gttgctttcc
      181 agttatctgc ccgaaggcgc tgtttccaaa ctgcgtgtcg atgccgtcgg agttggggtg
      241 gttcaacgcg ttgaacgatt cgacgcgaag ctgcaagttc aattgctcgg cgatacggac
      301 gcttttcacc gcgaaagcgc caggtcgaat gtgcagcgtc cgcgaatcac caactttccg
      361 gcgtcgccga aggagaattc cgcaggccgt gcaaacgctt cggtggggaa gtaccggaag
      421 tagcgtgcgc tcgtcggaag gcggatgcgg gttgccgaca acgtcaggcc gtgttgccgc
      481 ttggcgcggc gcattggtca gcgaaggctc gaacagccgg cctgagttga gggtccagtt
      541 gccgaaaacg ctccacgcgg aagcccctct gctcacggcg ccagagcagg tcccacctat
      601 ggcggcgggc ggcgttggca gagcttgcct gaagggtgct agctgttttt ccggctgtcc
      661 agaacctggc ggatccgtct ggcaagatct tcgggcgaga atggtttctg aataaactcc
      721 atcccggggg taagcatacc gccgggcacc agcgtattgt cggtataacc ggacatgtac
      781 aggaccttca tttccggatg ggcagccgtg gagcgctcag caaggtcacg gccgttcacg
      841 tgaggcatga ccaaatccgt aacaagcagg tctatccgct caccgcgatt aagggcgagt
      901 tccagtgctt cggcgccatg gcttgcggca agaacagtgt agccttggtt ctgcaacact
      961 tcccggacca actcccgtac cgtgtcctcg tcttcggcga gcagaatggt ttccgtgccg
     1021 cgcgcgggag ctgcgacgat ggcgcttgag gctggaatgg tcgcggcttc atcgacaatg
     1081 ggaaggtaga cgtcgaatgt ggaaccttcg ccaacgcgac tgcggaccgc gatctcaccg
     1141 ccactttgcc tgacgatccc ataagccgtc gagaggccaa gacccgtacc tttgcctttt
     1201 ggcttggtcg tgaagaacgg ctcgaagaca tgggaggcgg tttcggcggt catgccatgt
     1261 ccggtatcgg caacggagag cacggcatag cgcccggacg ccagcgccag ccccggttcc
     1321 ccggtctcgt ctttgattgt gatgctcctt gttcctatca ccagtctgcc gccgccgggc
     1381 attgcctcac gggcattaat gatcaggttc agaatcacct gttcgatttg cgcggcatcc
     1441 gccttgatgt ttccaagtga ttggtcgaga tccatccgga gttcgatatc ttcgccgatc
     1501 aggcggtcga gcatcgtttc gacgtccagg atgatcgcgt tcagatttag tacgcgagct
     1561 tgcagcacct gcctccggct gaatgccaac aactggccgg tcaacgcagc ggcgcggtcg
     1621 ctcgctttct tgatctcttc tatattgcgc cgcagcggcg ctttgtcgtc gagcacgccc
     1681 agaacgaggt cgctgtagcc cgcgatgatg gtcagaatat tattgaagtc atgagcgatg
     1741 ccgcccgcca actggcccac ggcatccatg cgctgcgatt gccgtagttg gtcttccagc
     1801 ctcttgcgct cggtaacgtc ggcgaccgtg gacatgatgc catagatatt tcctttccca
     1861 tcgcggagag gcgccgtcca gatcgtgacg tcaatgagtg tcccgtcttt gcggaggcgc
     1921 gatgtttcat acgccgccag ggtctcaccg tggcaaagac gctcgaattg ctccatactt
     1981 tcgggaccga gagcgggtgg aatcgtggga agcggcttgc cgacgatctc ggccgcggtc
     2041 catccgaaga tcttctcggc gcaggcgttc cacgacaaca cgtgccggtt acagtcgagt
     2101 gtgtaaatag caacgggcgc cgtttcgatc acggtacgca gggtcgcgtg agcctcgcga
     2161 agcccttctt ccgcccttct gcggctggtg atgtcacgcg agttgacgat gacgcccggc
     2221 tggcccgatt cgtcgatgaa atcctttcca atggcttcca acgtgcgcca gcttccatcg
     2281 cggtgccgca tgcggaactc cacggaccgc gccagcgcat cggaccgccc tgccccaccg
     2341 agcaggcggt tcaattgcgg agtgtcgtcg ggatgaacgt aaggcgctat tcgttgacct
     2401 tcgaggttct ccggctcgta tccgagaacg cgtgccacag ccgggctctc atagcggatg
     2461 acaccctctt tgctcaggat cgtgatcacg tcgagggcgt tttcaatcag cgagcggaag
     2521 tgctcctccc gttgctggag cgcttcctcg atgcgctttc gctcaatcgc gtagcgcatg
     2581 gcccgaagca gcaggttgcc atcgccctgt cctttcacca ggtaatcctg cgccccggca
     2641 cgcacggcct ttaatgccag ttgttcgtcg tcgtgtccgg tgagaatgac aatcgggatt
     2701 tccggaactt tctcgtgcac tcgaacgatg ctgtccagcc cctcagagtc cggcagcgac
     2761 aaatccagca gaaccacgtc aaatctctcg gcacggagcc gttcgatcgc ttcaccaagc
     2821 cgctgaacgt gagtcaggag cggcggcagt ctccgtgtgt ccacgatcgt ttcgcgaaat
     2881 agacgcgcgt ccgccgggtt gtcctctacc agtagaacct tgatttgctg ggtcatcgaa
     2941 ctcatcgcaa atgcgctcat cgcgtgttcg atcctcaccc ctcaattctt atgccgatct
     3001 gttcccgttc ccggcccccg cccgaaaagg gtgatctgcg gaagccgact gaccagtcta
     3061 aagagtctta cctctgtata gcacagcgag ttggaagacg atgcggcacc ggggttggcc
     3121 cgctccgccc cggccagtgc gacaggcaga attaacctcg acggataccg gcgtccgtga
     3181 taaatgcgct gcaccgccgt gtgaatttgc gtatcgtcgg cagcgcgacg tcccgtgttg
     3241 agattgcggt cgaacctggg aaaattactg ctggagatct cgagctggat gcggtgtctc
     3301 gtcttgaaca cgtggccggt gaccccggca tcgatggaga tctgatacac ctcgccgggt
     3361 atcgcgctga ccggcctctc aagggacttg cggtaccgga ggcggagtat tccatccgta
     3421 agattacgcg cgtagccgtc cggaccggca tcgacaagct tggccgtgaa atccgtatcg
     3481 ggagcggagg tagagacaaa caacacaacc cgcaccggcc ccgttgcttc cacgtcttcg
     3541 tccagttcgg gagttgtgta cacgagtaca tcgtggcggg cttcgacggg gcgctgatcc
     3601 atcggccccc agggaaaaac cttcgggttg cagcagaccg cgccgccccg ggtaggaacg
     3661 ggtttcagag gatcataaac gtagctgtcg aacgcctgat gccagtccgg cctgtccgcc
     3721 agaacaccgt ccccctcgat actgttggca tggccgccgc tcgaaaggta gaaaggaact
     3781 tcatgggagc gttccaaagg ccattcgcgt tcgtcgcgcc attggttggc gcccatcaca
     3841 aacatgcgca gcgggggttg agggaaagcg cttttcacgc cgcgcgggtg cggtttcagc
     3901 caatattcga accactccaa ctggagcttg cggagggcca gagccgattg cagaccaaaa
     3961 tccgtagctt cgaacttcgc ggacatgtcg tgtgcccacg gccctaccac cagccagtgc
     4021 cggcccgtct ttcccaaaac agagaatgct tcgaggtccc cctggacgta gttgtcatac
     4081 cagcctccga tggagaaagc gggagtgcga atcctagaca aggagtcgcg cgaccggatc
     4141 gttctccaat acgcatcata ggtagggtga tcgagcgcgc tctgaaaaaa agcgagcctt
     4201 tggcccgtgg ccgcgcggtc cgcggtccgg agcggcaggt gaaggacata gcgatcgaaa
     4261 ccgggactgg cgaaaccacg agcgcgaaag ctgcccgcca tccagagcag gcgatgtcca
     4321 agcttcaacg caccgccggg tgagtagtag cggtccaggt actcgtcgct gccggcgaaa
     4381 atcggaaaaa tggccttgag gttgggattg ttcatctcgg ccgctttcca ttgcgccatg
     4441 ccggcgtagg acgctccggc cattccgatg tttccattgc accatttctg ccgggcaatc
     4501 catttgatgg tgtcatcgcc gtccggcccc tcctggtcca tggagcggaa tgtgccgccg
     4561 gagtgatacc ggccgcggac gtcttgcaga acaatggcat aaccgcggct ggcgaggaag
     4621 ctgtaggtgc ccggttggct gcctttaccg tatggagtgc gaaccagaat ggcggggaat
     4681 cgggagctgg tagaggggag aaaaacgttg gtgcagagcg ttactccgtc gcgcatcggc
     4741 acgcggacat gcagcaccgg cacggcggca atcaactccg acgccaccgc cagaccggca
     4801 atccatcgca caagattcac ggcgcaccca cgcgggcaga gacccatgtt agcagcacgg
     4861 gaggggggtt ccacccgcgg ggcgcgccgg cagggctacg gctgttgcgg agcgatgtag
     4921 cggacctcgc gcattgcagc gagcgccggc agtttcccga ccgcgattcg gcccaccagg
     4981 agtttggcga cgcgcgggtc caccgttgtc tcgaacccaa gcgcgcggat ctgcttcaac
     5041 aattcgggtg aggcatcgga gagccacacc tggaggtacg ccttgccgtc gcggatgaat
     5101 gcggccgacg gcgtggcgcc tttcttcagt tgagagatcg cggccgcaac atccggatgc
     5161 agcctggata tcgcaagctg ttcctggctg tcgcgtctct tttccgcggg tactcgccgc
     5221 ggcgagggca tcggtgcagg gctgggtacg ccgccgatga caccccccac tacgcctccg
     5281 cgggcgaaac cgccggttgc ttgcatcccg gcatatccgg cgaagcctgt gcttctcaaa
     5341 cccgctactg ctacacgctc accgtaaata ccctcgtggc tgacgccttc gggcatctcc
     5401 accggcacct ccacccggcg cggctctcct ccctcgataa ccgtggtctc ttccaccgcg
     5461 acgaacgatg tgaactgcgt cacgaggcgg tagtccaagc cgagttgggt gatctgttcc
     5521 ttgagttgcg gctctacgtt tccacgttgg agcccggcat aatcgcggct catcagatcg
     5581 ccgatacgct gccgcgccca gaggctccgc agcacgttgt gacgcggctc ggatgcaggc
     5641 aggcttaccg tcatggtgcg ctcgaacggt ttgccagaca tggtgccgcg caagcgaacc
     5701 gtcccgcttc cagcacgcgc gtagcggccc gtgaggatga cgggtttcgc cgaaaacagg
     5761 tccggaatgc gctgcggata gacgtcgctg acctcgacac cgccgaagtc gatacgaata
     5821 tcggtgagca gcgggttgcg catgcgctca tgcaagcgcc gcgccgcagc ggagccgtcg
     5881 tcgttcaacc cgacgtactc cacttcaccg cgtccttcac gagccatatt gtccagcaga
     5941 aaacggttta cggaattgcc gatgccaaac gcaaagatcc gggcgttttt gtgcttctga
     6001 atctcggaga tgatctcgaa gtcgttgccc acgtagccgt ctgtcatgaa gcagacaatc
     6061 cggatatgat cctgcttgcc ggacggttcc agggcggcga tgatcgcttt catcatctcg
     6121 gttccaccca acccctgcct tgccgcgagg aactgttgtg ctttatccag gttttccctg
     6181 gtcgccggta cgggagccgg aaacagaacg tgcgtgtcgc ctgaaaatgt aatcaggtta
     6241 aacgtatcgc gcggatagag cccttcgagc gcgagcttca tggtttcctt cgccttctcg
     6301 atgggaaagc cgctcatcga cccggaggtg tcgagcacga acaccagttc tttaggcgtc
     6361 acatcctcca ccgtgaatcg ttccggcggc gtcagcatca gcgtgaaata gccgtcctgc
     6421 ccggtgttgt gcgccaggac ggcgtcctgt atcttcgcgc ccgatacgtc gtagtgcagc
     6481 acaaaatctt tgttgggtat caccaccttg tctttgagac ggatcacagc ggttgtggag
     6541 gacggtgcgt cgatggcgac atcgtgcgtc acggaccgta gtttttcgat gggcacgcca
     6601 gcgtcgagct tcacttccag cgagatatca tgacccgcgc gttctccttt ggcggcgacg
     6661 ggtggcgcga tgcggggagc atcggtaacc tgcctgggga tgtaacgcgg tccgacaacc
     6721 atcgggaatg aaaattcgta ggcgccttcg tcgaacttca gcgtttcgac gtagctgatc
     6781 tgaacggtga ctttggcacc cggcgcgata ttggccaccg attgcgtgaa gatattcggc
     6841 cgctcctgat caagcaagcc cgctacgttt ccctttgcgc gagccgcttc ataaatagcg
     6901 cgagcttctt cacgccgctt gatcttcgct ttgatgacgc ggtcgccggc tttgatcgtc
     6961 atatcgtcga ccgccgcctt gtccggcagc gggaacgtgt agacggcttc gatcttttcc
     7021 tcggatggat tttcgaactg ttgggtgacg gtgacacgcg cgatgaagcc gctgatttca
     7081 gctttgacat cggtgtgttt tagcgggcag gccgacgtca tctccccgcg cgggtcaatc
     7141 cggaacagcg ctccctgggt gaggtggccc tgggcggaaa ccgccgcttc cggatacacg
     7201 agataaagca aagcagcgcc gattgcgacc gagacgggtg ccacaacctt ctttttcatt
     7261 ccgttcctcc tgggatgtcg aatcccggtg aggtaatgcc acgaatgact ggatcctttc
     7321 actgctgttt tgcgccggtt acatcgaatt ctttaatcaa acgatccgga tgggattccg
     7381 gtgcaatgac gtacgtggat gccccacgtt gctcaacaag cggtggcgcc gcagcaccgg
     7441 cttcgagcgc ccgtatggcc agcgacccgg cctgtgccgg gctgtccggc tcgatggcaa
     7501 aaccaggatc gcgtttgcga gtcaggatca gcaggatatg gctctggccc gtcagcggtg
     7561 cgggaatgag caaggaatcc cacttcttta tatctccctg gtggacaagc cgccaaggtc
     7621 ccgcgatact tcttccgagc acttgaagaa aagcgggttc gttggactgc acctgcaatc
     7681 gcgctctctg ttgtgacgga acaatgctgt agcgcaaacc gagacgcgca ggggtgtccg
     7741 cacggcgcgt gaccgcggcg gttggcgccg gctgccggaa ccccgccatc ggttgtacgg
     7801 cggaaatggc acggccgtag aaccaggcgc tcgcacttga aggtgcggcc ggggcttgcg
     7861 gctctgccgc gtagccgccg agcgcgcccg gaactcctcc cacaacacct cccaccactc
     7921 ccacgggagc ttcgcccaag cgcgtcgcgc ggctcaattc acttttcccg ggtggaggtg
     7981 gcaccgaaga gagatcccgc tcggcaaaca tcggcgatac cgttttcgag gcggagcgca
     8041 gttcacgctc taccgggatc gcgggtggcg gcggcaaagc cggagggcgc ggaggcgcgg
     8101 cgcgttctgg cagaatttct gctttccggc gcatcccggc gcttggcgtg gcgggctgtt
     8161 tcggtgccgg tggttccgga gcgggcgcct ccgcaggcgc cgcttccggc aatgtgggcc
     8221 gatggctctg cgccaccagt tccggctggc gaatttcggt atggtcgaac cggtagacga
     8281 cgaagaacgc cagagcggcg acggctacgc tgcccgccaa ggcaaccgga gccggcttca
     8341 gaacgttgtt ccagaagcgt gcgaaccatc ctttttcgtg tgaatccagc gctcccagca
     8401 actccgttcg catcgctgga tcggtcaaca gctccagcag cggctgttcc tccatgagta
     8461 gctcgaatag ctcggagtcc tccaacgccg cttcgaaaag cgccttccgc tcctctggcg
     8521 tgagagttcc cgcggagtat ccgccgagaa gttttctggc ttcgcccgag ctcacttctt
     8581 tgtctcccaa ctgcctccga tcagttcaag cagatgctgc cggcagcgat agtcccacgt
     8641 gtaaacggta ttcagggagt gggctcccat cgctttccgg atctcgctga aactccttcc
     8701 ctgtaacttg agacggagca gctcacggca gcgttgccca acctgcggca gcgccgcgcg
     8761 aatccggtcg agcaactcgc gccgggaagc gagaatctcc ggatccggtg cgaaatccgg
     8821 aagcgggatt tcgtccaccg agaccgccga gtcttcaccc cggcgcctcg ccttccggta
     8881 gagcgccgta atcttgaagc gcgcgatccg catagaaagc ggaaccaatt cctcgagggc
     8941 ggtgacgtga ggatatttct cttcgagcag gagcaggact tcgtgcgcaa cgtcctccgc
     9001 gccgtccctc attacgcgag atgccgcgaa cgcaacgatc ctttcacgaa gcctggcgag
     9061 aatttcgtcg cgttccacgg caaaccatat tagacggtat cgcgggattg ggggtttccg
     9121 gggcccatag cttccctctc gaaaatgtga aataattcac ttactggaaa aaccagggag
     9181 ttgaagcttg taagctcaca aagcttaccg aaaacgaaaa ggtacagaca atgaaaagga
     9241 ccatcctagc aatattcttg ttcgctgcga tggcggtggc tcagacctac tacggcacaa
     9301 ttcgtggtac ggttatggac gccaccggcg cagtcgcacc gggtattgag gttattctca
     9361 ccaacaccca aacgaatatc gaaaccagga ccgtcagcaa cgaggtgggc aactacgttg
     9421 ctccgaacct cattccaggc agctaccgcg tctcggtgga ggcgcaaggt ttcaagaagt
     9481 tcgtcgttga aggcgtcgaa ctggtcgcga ctgccgaccg ccgcgtcgat gtccggctgg
     9541 aagttggcag cgtagcagaa tcaatcgagg ttctgggcgg cgctcagttg atcgaaacgg
     9601 agcgcgccac gatttcggat gtgaaatcga atctcgtgtt cacaactatg ccggtggttt
     9661 ccaactaccg gtcgatctgg cgcatgttgg ccctcacccc cggcgtaacc ggcgatggaa
     9721 gctattacgc aggcaacggc cagggccgta atacaacgta ttctatcgat gggattccga
     9781 tcaaagacgg ctggaccggc agttcattcg gaccggcgct gacgtacctt gattcctacc
     9841 gcgagttccg tgtggacatg gtgagcgtga acgcttccgg aggcacgtcg tccaacgtcg
     9901 cagtggtttc cgaaagcggc acgaacgcgc tgcatggcga agcgtggctg cactacaacg
     9961 caatcggttt tggcgcgcgg ccgttcttct caccgcgggc ttcgagcggc ccgccgattt
    10021 accggccgaa cgtgaaggcc ggcggaccgg tggtactgcc gaaactttac gatggcaagg
    10081 accgcacctt ctggcatttc agttggcagg gcattcgagg cagccaggtg cccgccgtca
    10141 ccaatatcgt ggtgccgacg gcagcgttcc gtgccggcga tttctcgagt cttacgacgg
    10201 cgctgagaga tccgctcacc aaccagccgt tcgccgataa ccgcattccg gaaagccgga
    10261 tcagcggcgt ctccaagtat taccaggagc gcttctatcc gctgccgaac agcggcgcgg
    10321 accggtacac cgacgtcaat gtctcgccca acaccagcga ccagtacacg gcacgcatcg
    10381 atcacaaact gaccgatgcg cattctttct tcgggcggtt catgtaccag cactacgcgt
    10441 ttgaacggtg ggacggcgag aacaacccgc tgatcggaaa ctatgaacag tggcgggacc
    10501 agtatcactt tgttctctcc gacacctacg tgttgtcgcc gtcgacgatc aacgagtttc
    10561 gcgtgggata cgcccgcgac gccagcatct acggcggaaa gaatcgtgga ctcgacatcg
    10621 tgcgtgaatc cggtttgcag ttggccgacc tccaggattc ggccggactg ccccggatga
    10681 atatcaccgg tttctcgtcc ctttaccagg gcaacgagag cggctggacc tggagcaact
    10741 actacgtgca ggaaacgctg cataaaatca tcgggaagca taatctacgt ttcggcgtgg
    10801 acatcggcga ctacaacggg cgcatgatca ccacgtcgcc cagcgccgtt tacggcatat
    10861 acggattcaa cggacgattc tccggaaacc cgtacgcgga tttcctgctc ggcatcatgg
    10921 atacgagttc gcggcagacg tcggtgggcc cggtgtatcc tcaccgttcg acgtgggagt
    10981 tcttcgtgac cgacgattac aagatcaccc cgcgattgtc gctcaacctg gggctccggt
    11041 actcgctgtt gactcccgga acgatcgagc aggacctgct ggcgaacttc atcccgtcag
    11101 cgaacgcact agtggtggtg gatgatgagg tgaaagcgcg tatccacccg ggattcccct
    11161 ccaacgtacc gatcgcgacc gcttcctcgc tgggtttaga caggtccctg ctcaaactgg
    11221 accgcaataa tttcgcgccc cggttcggct tcgcgtggcg tcctggccgg tgggaggact
    11281 tcgtgatccg cggcggcgca ggtgtgtact acgtcgccat gcagccgtac gtttccgacg
    11341 gcggcggatc gcccttcgag ctgtctgaga gtttcaccaa ttcggttacg aacggcgtgc
    11401 cggccttcca attcccacgc ccgttcccgg ccacgggcta tgtccttggc gggaccagcg
    11461 cgaacggtat ggacccgaac ctgcgaacgc cgtacagcac gcaatacaac cttaccgtgg
    11521 aaaagcaggt ctttgatatg ggtctttcgg cgacctacat gtccaccctg gcgcgcaaaa
    11581 aggtatggac gcgaaatctg aaccaggttc ccgccgacac gcggccctat tccgagaaac
    11641 tgctgtccgt gcccttcccc tacctcttct cggccaacga gacgattaac gggtcgtttc
    11701 acaactatca tgcgggcact atcaaggccg agcggaagtt taaagacggc ttctactatc
    11761 aggcgcacct gacctgggcg aagagcatgg ccgacgattg gagcggcacg gaagacgtgt
    11821 tcaaccgggc tcgcgaacgc tcgcaaggcg gccagattcc acggtggcgg ggcgtcgtcg
    11881 tcggcatcta tgaaatgccg ttcggacatg gcaagaagtt cggctccagc atgccgcgcg
    11941 ccttgaacta catcgccgga aactggacca tggccggcac ctacgtgtgg caaacaggca
    12001 gatacttctc gcccgcattc gacggcgtcg atccatccaa caccaaccgc ttcagcggcc
    12061 gtccggaccg cgtggcggac ggcaacctgt cttccgatca gcggacgctc gggcagtggt
    12121 tcgacacgtc cgcctttgta gttccggcag ccgacatcgg ccggttcggc aattcgggag
    12181 cgttcatact ccagggtccg ggactgagcg tgttccactt tggcgtcaac aaggagatcg
    12241 tgttacacga gcgggctcgt ctgaaactgg agatggcctc gacgaatttc ctgaaccatc
    12301 ccaatttcgc caacccgtca gcgaccatcg gtacgagcgc ctacggacag gtcctgggaa
    12361 ccatcggaac agacggcaac cgggacttcc aattaaccgc ccgtttgacg ttctaaacgt
    12421 ggcatcagaa tgagtcgcgg ctcagcattc gtttccgagc cacgaccgca agggagtggt
    12481 tatcgggcgg atccgatgga agttggcaga tccgcccgat ctcccgtggt gataagatcg
    12541 ctccatgtgc acactgtccc gccgggcctt ttttgccgct gcggctgggt tctccgcagc
    12601 gatctccgcg ccgggggcgc ccgtaccggt tgctcaatac cgccggggcg gcatgatcta
    12661 tcgccgtctg ggccaaacca gcctctaccc gtccgtgctc tcgttcgggt cgcacaccga
    12721 ccccgcttat aagcgcaaag taaaacacgg cagtgtcctc acagaagagg gacaacagcg
    12781 gcgcgaccgg atgctcgcgc gtgccctgga ccggggcgtc aacatggttg atacctacga
    12841 gaccgaaggg cagtgggaac acgtggcgcg ggtcatccag tctcgcaggc agtcgacgct
    12901 cgtgtcgctg tgccggcagc ttcccatgtt cgttggcgag aatatcgacc gcgcggcacg
    12961 cctctacggg cacgtcgaca tgtaccgcat ctacatcaac gatggtgcgg cgctcgatgc
    13021 caggggcatt gccgattggg atgtcctgcg caaagcgaag gaggccggca aggtgcgcgc
    13081 catcggtatc gcgacgcatg acgagcggct gatggttgcg gcgctgcgcg agttggaagg
    13141 catcgactac atcatgttcc cgtacaactt cattcacgcc cgcgccgact atacagagtt
    13201 ccttcccgca gccatagcga gcggcgttgg gctgatcgcg atcaaaccgc ttgcggccgg
    13261 ctccatcgtc aagctggata cacgtgtggt gaagggctcc cgccctgaat ccgatgtcgt
    13321 gcagctctat cagaagaagt accgcccgtt gcatcccgcc gtcgtctccg agctgacaag
    13381 gagcctggcg cgcctgcccg acgagactct ctgccaggcc gctatgcgtt tcgtgtattc
    13441 gcgtcctttc ttaacctgcg cgatgccggg catgttcgaa gagcacactc tcgaagagaa
    13501 ctatgccggg cttacgcgcc acctggaact ttcgcgcgag gagcgcgcgg ccctcgatgc
    13561 cgccgcccat tttgcccgcg ccacttccgg aaattggctc acccctggct accgatggct
    13621 cgatacccgc tggtccccca cgcgcgcctg actcctcttt tactgctggc agcactcgca
    13681 gcggcggcgc agacctctac gaatcccgaa cgcttccagc aggcaggtgt ctgctcgcgt
    13741 tgtcacgtgg cgcaggtgct ggagtggtcc atatccggac acccgcgcgc cgccaccggc
    13801 tgcgaggaat gtcacggtcc ttccaaggct cacgtcgtcg acgaacgaaa tcaggtgaaa
    13861 ccggacaaga tgccgctggg tgccgcgatc gccggattat gccagtcctg ccatacgcag
    13921 ggatgtccga agacggagcg ccgcgcgtcc tgtgaatcct gtcacgatcc gcacgcgctg
    13981 tttcatccca aggcgaccaa ccggcccgcg cccggaagcg aagatgacgt gctcccccgg
    14041 ttcgaatcgc acctcaaaca aggtgagagc cttattgaac agcggaactg gaacgccgcc
    14101 cataaggagc tacaggccgc tctgcgccta tatccggacc acccgcgggc ttccgcgcgt
    14161 ctcgcactgg tgaaccgtcg cttgaacccg agccttccag gcttcgatat ccttgacgat
    14221 cagttcgatg ccgaaacggg tcttcccttg cacgttcgcg tttccggaat gccggtggag
    14281 atggtgctca taccgggcgg cgaaacggac ctgggcaatg atgcgcagcc gtccgcgcgc
    14341 cctgtacaca cggtctccat tgctccgttc tatctcgcgc gaacggaatt gacgcagggg
    14401 gtgtggatga cgctcgatcc cgccaacccg agcgttcacc gcggggacga tcttcccatc
    14461 aacaacatct cctgggagga tgcacagcgg tggattgcaa aactgaacgg gcgcattgcg
    14521 ggcggtggct tccgcttgcc gtccgaagcg gaatgggagt ctgccgcgcg cgccgcatcg
    14581 aaagacatcg attccggtga acgcgcctgg taccgggaga actcgatggc ggcggcaggt
    14641 tcgggcgagt ttcgcgagct cgacgcctat gccccgcgcc gcgttgccgc gaaacggtcg
    14701 gacggccgcg gcattcacga tctggcaggg aatgtgtggg agtggtgctc cactttgatg
    14761 aaaccttatc cctacgaccc gcgcgacgga cgggaatcgc ccgatacgcc cggtttacgg
    14821 atattgcgcg gcggcggctt cgccgattcc gccgattacc ttgcaccgtg gttccgccat
    14881 gccgagcggc gtgaccgccg cctgccattc aacggtatgc gcctggcccg ctctgtgccc
    14941 gcgcagtgaa gctgccaatc agccgaagaa aatcgattcg gcggttcgag tgagtaacca
    15001 ctccttgtct tcagcggaca gaaacggaag tccttcgcgg atcagcgcga tggatgccgc
    15061 gatcgtgtgt tccttctgca cctgaaaagg acagtcgctc gcccacatca accggcgagg
    15121 gccatagtcc tcaaacacgc gccggatgag aggggcgagg tcggcatagg gaggcgtttt
    15181 gcggccgagc gcataaaacg ccgagacctt caccgccacc ttcggatgtt tggatagccc
    15241 gcataacagc cggacgtcgg catcgcgaat ctctcccgtg gcgccgatgc gcgccatgtg
    15301 gtcgatgacc acgggcgtag cgggatgccg tatgcacatg cggtcaatcg acggaagggc
    15361 atccgggccc accagcgggc acatcgccag gttctgctcc gcgccgcagc gccacatcgc
    15421 ttccatgccg ggcgtgtcga gccagctttg cggcgaagcg ccgggcgtaa tccggaatcc
    15481 gcggacgccg tgtttcgcca ggtcacgcat agtcttctcg ggatgcggcg aggacgagtc
    15541 gacgatgccg acgcccgaga acacgcccgg gtaggcgcgc atcgtatcg
//
LOCUS       DASTMK010000347        16292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_273381, whole genome shotgun sequence.
ACCESSION   DASTMK010000347 DASTMK010000000
VERSION     DASTMK010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16292
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273381"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFQ79_15205"
     CDS             <1..364
                     /locus_tag="VFQ79_15205"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HEU0141069.1"
                     /translation="PSAHTTFVTEGAVICSFVPRPLEEDAEAIRVPFFHRNTDYDEFI
                     FYHDGDFFSKDNIKPGMATLHPRGIHHGPHPKALAGQTKKTHTDEYAVMLDGLNPIRV
                     LPAGAQVEWQDYWKSWKG"
     gene            492..1163
                     /locus_tag="VFQ79_15210"
     CDS             492..1163
                     /locus_tag="VFQ79_15210"
                     /inference="COORDINATES: protein motif:HMM:NF016733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HEU0141070.1"
                     /translation="MFANRVISKLFLGIACMVPFVAHANLLTNESFEAGAFVNQGNAT
                     MVLPVGSTSITGWTVINDQLAWIDIGNPWGLTAQDGDRFLDLTAYPTGAPFGGVSQTI
                     TTVAGQQYMLSFYLGTYTARWGGPPVSISASAGNASQTFTVSTTSTASTWTPFSMPFT
                     ATSSNTAISLAGSAGFQYIGLDNVSVESVGGSPVPEPGTYALVIVGLMLLAAGGRRRH
                     GCSDS"
     gene            1295..1759
                     /locus_tag="VFQ79_15215"
     CDS             1295..1759
                     /locus_tag="VFQ79_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141071.1"
                     /translation="MEAKPTHEQAQLHLQVYEMRREARLRQARDWFFQNYFADTFDEA
                     MRIAAPGTETGSFVMMVFSYWEQACALLNYGLLHEDLFFETSGEFYGVWQRIKPTIQE
                     GRQRFASNQFLAHVEKAANRYEAWTEARSPGHIAAMQEMMKQMRPRSGAARS"
     gene            1921..2529
                     /locus_tag="VFQ79_15220"
     CDS             1921..2529
                     /locus_tag="VFQ79_15220"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU0141072.1"
                     /translation="MIIDPGAESPRNVYKVMIGAIVPRPIAFVSTLGADGIRNLAPFS
                     FFTAISANPPVICFSPMIRGSDSGRKDTLRNIEATREFVVNIVSENFVQQMNTCSGEY
                     PPEVDEFEVSGLTPVPSDMVRPARVKEARVNMECKLVQVVHVSERQLGGSIVLGEVLR
                     FHVDDTIVEDFRIDPDKLSAVGRMGGATYVRTTDRFDLVRPK"
     gene            2526..4460
                     /locus_tag="VFQ79_15225"
     CDS             2526..4460
                     /locus_tag="VFQ79_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0141073.1"
                     /translation="MSSRWIWEPPAGWVEETNVHRFMKKLGTESVDEFLQYSRDHLEE
                     FWDAMVRETGIEWFEPYSQVLDTSEGIEWARWFLGGKTNIAWNCLDRHRMSDRIACIG
                     ETENGAVRRISFRELYRDVSRLANALKALGLERGDRVALTMPMVPEVVTILYACHKLG
                     LVVVPIFAGFGAGAIATRLQDSGARVLFTADRLERRGKFIELKEKTDAALDNCPTVSH
                     AVVLRYRGGDVAWKEHRDIWWDDFVAGQPEECESLRLEGDAPAFILYTSGTTGKPKGT
                     VHTHAGSLAQMTKEIYLAFDHRDSDIFFWVSDIGWMMGPWTIVGNHNFGGTIFLYDGA
                     PDFPSPDRLWQMVERHGITTLGISPTAIRMLMRHGSEIPERYPMKSLRLLGSTGEPWD
                     ETSYLWYFEKAGKGRLPIINISGGTEIAGSFLFVLPVQPLKVCTLGGPAPGMGTDVVD
                     GTGRSVRGSTGYLVCRTPAPSMTRGIWGDPRRYIESYWSRWKGMWYHGDWARVDEDGH
                     WFVHGRADESMNVAGRKVGPAEVEQALMQHASVAEAAVIGVPDEVTGEAIVAFVVPRP
                     GASAVSEELCRHVAAVLGPAFRPRSVEVVSQLPKTQSGKVVRRLIRQYYLGEAPGDLS
                     TVENAEALRQFGDQGPSRRG"
     gene            complement(4457..5329)
                     /locus_tag="VFQ79_15230"
     CDS             complement(4457..5329)
                     /locus_tag="VFQ79_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0141074.1"
                     /translation="MFSLAKIILPVDFSERSAGAARYVEALATAFGSEVFLVHVLPPP
                     HYEFSALEVGGPVLNELFASRGTQVKKELDDYLREELSRLEVRRVLLEGDPATELVKF
                     ASEQKAGLIVMPTHGYGPFRRFILGSVTAKVLHDADCPVWTGVHLEQAPAVGAIQFRN
                     ILAAIDLGPQSKRTLTWAGEIAKRFDASLRVLHVMPCTVAGNDEEAIAAWKSEAVTKA
                     TEAVHEVAAEAGIEAKIKIETGDAPKTVCDHAQRIGADLLVIGRGSAAGVFGRLRTNA
                     YGIIRESPCAVVSV"
     gene            5557..6414
                     /locus_tag="VFQ79_15235"
     CDS             5557..6414
                     /locus_tag="VFQ79_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU0141075.1"
                     /translation="MDSHDFEFSTPGTLEELIELLSDPEKKPLVGDMSVIPLMKLRFA
                     RPSAEAGLRSVRGLNEIKEEGGSIRIGPAATHLQIESSPLLRNRCPLLPETATHAGGR
                     QTHSAGTIGASVSHSDPVSDYPAALLALEAKLHVVGKNSDRIVAARDFLSNTAVMALE
                     PGELLMETTVPVEDSSTGVHYERVSNPDSDFTVVGVAARIRRAEGKIAMARVGVTGLA
                     ARAFRAVNVEQALEGTPGSETDISNAAALVGEGVDARSDVYGSADQRRQLARLHTERA
                     ISEALRKAA"
     gene            complement(6418..7683)
                     /locus_tag="VFQ79_15240"
     CDS             complement(6418..7683)
                     /locus_tag="VFQ79_15240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0141076.1"
                     /translation="MILRYWTAMAGGCLIAAIKALLPRKAGLPRLPDFPSGISVVIPS
                     RNGQDLLERLLPRLVAELDGIPSEVIVVDNGSDDETATWLSSAYPGIQVLVSQEPLSF
                     AAAVNRGIASAHFSHVCLLNNDMVVGAGFFHALRAAFENVPDLFSATAQIFFPEGVRR
                     EETGKAVMPPLRPASEFPLRCDEPVPGEDLSYVLYASGGCSLYDTAKLRQLGCFGEMY
                     RPAYVEDLDAGYRAWLRGWPTVFVAGATVVHHHRATTSRYYSEDELRLVTEINYLRFL
                     ARAVVSPRLFLKLWTEAVRRLRTDPVIAAPALGFAARAFRWVERPPVCVLPEHYVLAL
                     GSGNIAVFPGRKPSNKPVVITTEPNVRTNDACVLVCLVDELRPEPEWLQSHVEVVLVR
                     RTRTAFDSTMLAAAISESRRKWNAVVPAP"
     gene            complement(7691..8782)
                     /locus_tag="VFQ79_15245"
     CDS             complement(7691..8782)
                     /locus_tag="VFQ79_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141077.1"
                     /translation="MPQIADPLCLAGAWFLNSGIQEPEGGVARYYHSDTGINARVSTE
                     ITGYAVSTLVYLAAKTSWPDYLNAGLKAARFLTRDAWDSAASQFPFEYSSSKSDPPKP
                     LAYFFDTGIILRGLLAVWRATGQNEFLERAAEAGHAMHAGFVRGHVIHPVLNLPDGSP
                     VPHGESWSRNPGCYQLKAALAWRELGEATGDSSFTGFYEHALQIALRQESDFLPGVTE
                     RERVMDRLHAYCYFLEGLLPEAPRQECAEALRRGIAKVARLVREIRPIFERSDVWAQL
                     LRVRLFADALGVLKLDSASAEEEAELLSSFQLQEPADSMRGGFWFGRKQERMLPYVNP
                     VSTAFALQALQMWREVHDGQFHTGSRELI"
     gene            8879..9523
                     /locus_tag="VFQ79_15250"
     CDS             8879..9523
                     /locus_tag="VFQ79_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141078.1"
                     /translation="MRFLAGMLAVSGLVAQDGAPAGIVKGELIEWRGSDTRGEISLRA
                     PDHRVYWCSFDEKTYFERDGKRTLISKAAAREKIELLSDRGPAPSGCYARLIRVMEEK
                     HVPPAYKIRTMLRPERRSWQTDTLFPRGNLTFAGVVLWVRPELFAVRMNGGEQKKFVL
                     REDTRYAADGQTVNASMLEPNTRVFIRAGKNLDDQLEAFHIVWGRILLPGGSGH"
     gene            9539..10759
                     /locus_tag="VFQ79_15255"
     CDS             9539..10759
                     /locus_tag="VFQ79_15255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0141079.1"
                     /translation="MTYDAIIIGSRVAGAATAMLLARGGYRILVVDRARFPSDTISTH
                     IVWQPGVARLIGWGLGEQLIALEAPPIDTIEFDLGPFSLAGVPPPSGPAESAYAPRRT
                     VLDSMLANAAAAAGADVRDGFTVDEIVFDGDRVAGIRGHGESGAVVVEHANVVIGADG
                     VHSILARAAGAPEYNVHPPLTCWYYSYWSGVRNDRIRFFSREGRAFGCIPTNDGLVCI
                     PVIWPHDRFAEVKRDIERHYLSSLDLAPDFKKEVLAGKREERFYGTAHIPNYFRSPYG
                     HGWALVGDAGYHKDPILAQGISDAFRDASLLAAALDDVFSGRSLWDPALGGYEAARNS
                     AVAATYEMNTQFAALAPPAPEMQALMAALRGNQRDTSQFIGTMTGTVPVAEFYAPENV
                     QRIRNYGDISATAC"
     gene            complement(10824..11339)
                     /locus_tag="VFQ79_15260"
     CDS             complement(10824..11339)
                     /locus_tag="VFQ79_15260"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0141080.1"
                     /translation="MPTHAELFIAFSSGKLRQLAGRIEQCLEKLDDSQIWARGSDAEN
                     AVGNLVLHLCGNVRQWIGYGASGLDDIRERDREFETHGGISRAELSAKLSGTVSEAAS
                     IIDTLPAARLLERTKIQNYDTTVLEAVYHAVEHFAHHTGQIIFATKMLTGSDLGFYRH
                     LAKPAHTEKVP"
     gene            complement(11342..12766)
                     /locus_tag="VFQ79_15265"
     CDS             complement(11342..12766)
                     /locus_tag="VFQ79_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0141081.1"
                     /translation="MPEHFRPTRNRREFLCDAFCGFGTLAFASMLAREDARAAMKNPL
                     APKPPHTPDTAKAKSVIFLFMAGGPSHLETFDPKPLLNKLHGRKRPAAFGEAKYQFIS
                     KNALLMGTKRTFKKHGVSGIDVSDLLPHIAECVDDLAVIRSCHGDMVVHSAAQYQLMT
                     GRVIPGFPSMGSWVIYGLGAEAESLPAYVVLPDPKGALEAGQPMYGNGFLPAIYQPSM
                     FRPGRRPVLNLDLPSGVALEDRKKTLSLLRQLNEANMRAGDEEFEARINAYDLAFKMQ
                     TEAPAVFDLSTESQETLDLYGIGRQTTDDYGRRCLLARRLVERGVRFVCVVSGGGPGN
                     LQWDAHTDIEENHLRMAAQSDQPCAALIKDLKRRGLLDSTLVVWGGEFGRSPEAEGGK
                     GRDHHNLGFSMWLAGGGIKGGQVVGATDEIGLHAVEKPYHFRDVHTTILHQLGLNQDA
                     LSYMHLGRKERLTEVHGHVIQEIV"
     gene            complement(12769..14742)
                     /locus_tag="VFQ79_15270"
     CDS             complement(12769..14742)
                     /locus_tag="VFQ79_15270"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEU0141082.1"
                     /translation="MCVAAVYPLPASDEYAPLGSYTAAERRHWAFQKRKNPEVPVFSD
                     AAGKAWVKTPIDAFVLEKLRKEGLEPAPPAARRILIRRVYFDLIGLPPAPAEVENFAS
                     DKLPGAWERVVDRLLASPHYGERWGQRWLDVVRYAETDGFEYDTHRNGAWRYRDYVIR
                     SFNEDKPYDRFVKEQLAGDELEPGNQALQIAAGFQRLGPLRKNAGNQEVASSRNEVLT
                     EMTNAVGAALLGVTLGCARCHDHKFDPIRQADYYRIQAYFAATHEKDVSLASEAEQAE
                     WKKKSGAFQEQLKKLRQSMKGLEGSAKEEIEQQVKDMEARAPEPLPAIFSVSNDPARI
                     TPVHLLSRGDHLRPGARVGMRPLGVLLEESVPEYTAVPENPRTRLAEWITSPENPLTA
                     RVMVNRIWQGHFGRGIAAMPNDFGRMGGLPSHRELLDWLANQFVEGGWRIKPLHRTIL
                     LSSAYQQSSHSPIGKAAREKDPDNKLLWKYSRRRLEAEEIRDAMLAVSGHLNLKQGGP
                     GVMPPVDQELIDLLYKPSQWAVAEEASEHHRRSIYLIAKRNLRLPFMEVFDAPDLQIS
                     CPRRESSTHPPQALELLNGRTANEMAEALAARLRKEAGISRTRQVELAYRFALGRSPS
                     KQESQLAVSFLRGAPLREFALAVLNLNEFLYVE"
     gene            complement(14933..16102)
                     /locus_tag="VFQ79_15275"
     CDS             complement(14933..16102)
                     /locus_tag="VFQ79_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU0141083.1"
                     /translation="MRLTEIQEALRQEQIDGWLFFDHHQRDELAYRILGFTPVRHVTR
                     RWYYLVPANGEPRALVHRIESSMLDSLPGEKRPYASWQQQAAGLTYLLEGCRRVAMQY
                     SPDCAVPYVSLVDGGTLELVRATGVEVVTSADLVQRFEASWNSNQLESHLEAGRRIDR
                     IRAAAFQEIGGRIRANSRITEWDIKQFILREFAANGLVTDHGPIVAVNANCSNPHYEP
                     TAEAPQEIRSGDFVLLDMWAKLDTPGSVYYDITWTGYCGSEPPEKMREIFDIVRRARL
                     RAVETVQRAVQTRQPLRGFEVDDAARGFIREKSYAEYFFHRTGHSIGMDVHGTGANMD
                     NLETHDERRIIPWTCFSIEPGIYLPEFGVRSEVNVFVGDEEAIVTGESQDELVRI"
     gene            complement(16089..>16292)
                     /locus_tag="VFQ79_15280"
     CDS             complement(16089..>16292)
                     /locus_tag="VFQ79_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141084.1"
                     /translation="AISSILLGTIITVVWNLFEISTLDAIYPALGASVASLILVSLLT
                     PPPPPEKVDPFFSEPVPFQHETH"
BASE COUNT         3277 a         4712 c         5010 g         3293 t
ORIGIN      
        1 acccagcgcg cataccacgt ttgttaccga aggcgcggtg atctgtagtt tcgtgccgcg
       61 gccgctcgaa gaggacgctg aggcgattcg cgttcccttc ttccaccgca acacagatta
      121 cgacgagttc atcttttacc atgacggtga tttcttcagc aaagacaaca tcaagccggg
      181 catggcgacg ctgcatccgc ggggcattca tcacgggccg catccgaagg ccctggcggg
      241 ccagacgaag aagacgcata cggacgagta tgcggtaatg ctggatggtc taaatccgat
      301 ccgggttctg cccgccggcg cgcaagtaga atggcaggac tactggaaga gctggaaggg
      361 atgatctgag caggccgcag caaggggttc ctagtactct caatttgcag ccggatagct
      421 ccgatcgcac gcaaaatcgt ggacaggaag gcgaccgcca gagtagaatc catgctattc
      481 ggaggcgcta tatgttcgcc aatcgggtga ttagcaaact attcctgggc attgcatgca
      541 tggtgccctt tgtggcacac gcgaatctgc taacgaacga aagcttcgaa gcgggggctt
      601 tcgtaaacca ggggaatgcc acgatggttc ttcctgtggg ttccacgtca atcacgggct
      661 ggacggttat caatgaccag ttggcgtgga tcgatatcgg taatccgtgg ggtctcacgg
      721 cccaggatgg tgatcgtttc ctcgatctca ctgcgtatcc caccggagct ccgtttggcg
      781 gcgtttcgca gaccatcaca accgtcgccg gacaacaata catgttgtct ttctatctgg
      841 ggacgtatac ggcgcggtgg ggtggaccgc cggtatctat ttcggcgtcg gcgggtaacg
      901 ccagtcaaac tttcacggtt tcgactacca gcaccgcatc tacctggaca cccttctcca
      961 tgccttttac ggcgacttca tcgaacaccg caatctcgct ggccggttca gccggatttc
     1021 aatacatcgg cttggacaac gtgagcgtgg aaagtgtggg tggaagcccc gttccagagc
     1081 ctgggaccta cgctctggtc attgtaggat tgatgttgct ggctgccggc ggccggcgcc
     1141 gccacggttg cagcgattcg tagaaaattt tcgaagttct gtcgatttcc cggtctttca
     1201 cgcgatgtat ctatgaggcc tgattctaac cgggccagcc cggaggaaag gcctctttcg
     1261 gagaaaacgc tacatcacgg aaaggaaatg actgatggaa gctaaaccaa cacatgaaca
     1321 agcgcaattg catctccaag tctatgagat gcggcgcgag gcgcgattac ggcaggcacg
     1381 cgattggttt ttccaaaact actttgcgga taccttcgac gaagccatgc gaatcgcggc
     1441 gccgggaacg gagaccggca gcttcgtcat gatggtattc agctactggg agcaggcctg
     1501 tgcgctgctc aactatggct tgctgcacga agacctgttc ttcgagacta gcggtgagtt
     1561 ttacggtgta tggcagcgca ttaagcccac catccaggaa ggccgtcagc ggtttgcgag
     1621 caaccagttc ctcgcacacg tggaaaaagc cgcgaaccgg tatgaagcct ggacggaagc
     1681 ccgctctcca gggcacatcg ccgctatgca ggaaatgatg aagcagatgc gtccacgatc
     1741 gggagcagca cgtagctgac ccgttccatt tagactgaat tcaccggcgg acccgcgatg
     1801 ccagaggcct ccagtgcctc cggcccgcgc gggttccgca catcctccat cctgctgtcc
     1861 gtcccgggag cgaacttctg aaaaggcgcg cttaatgaga gaataatagc cctgcacgct
     1921 atgattatcg atcccggcgc ggagagcccc cgcaacgttt acaaggtgat gatcggggcg
     1981 attgtgccgc gcccgatcgc gtttgtttcg acactcggcg cggacggtat tcgtaacctg
     2041 gcaccgttca gtttcttcac ggccatcagc gccaatcctc ccgtaatctg cttcagtccg
     2101 atgattcgcg gaagcgacag cgggcgcaag gatacgcttc gcaatattga agccacacgc
     2161 gaattcgtgg tgaatatcgt ttcggaaaac tttgtgcaac agatgaacac ctgctcgggg
     2221 gagtatccgc cggaagtgga cgaattcgag gtttcggggc tgacgcctgt tcccagcgac
     2281 atggtgcgtc cggcgcgcgt gaaagaggcg cgcgtgaaca tggagtgcaa gcttgtgcag
     2341 gtggtacacg taagcgagcg gcaacttggt gggagcatcg tgttgggtga ggtactgcgg
     2401 ttccacgtcg atgacacgat cgtggaggat ttccgcatcg atcccgacaa gttgtccgct
     2461 gtcgggcgaa tgggtggcgc cacgtacgtg cgtacgaccg accggtttga tttggtgagg
     2521 ccgaaatgag cagccgctgg atttgggagc cgccagccgg ctgggtcgaa gagacgaatg
     2581 tccaccggtt catgaagaag ttggggaccg aaagtgtaga cgagtttctt cagtattcgc
     2641 gcgaccacct ggaggagttc tgggacgcta tggttcgcga gacgggaatc gagtggttcg
     2701 agccgtacag ccaggtgctc gatacgtccg agggcatcga gtgggcgcgc tggttcctcg
     2761 gcggcaagac caacatcgcc tggaactgtc tggaccggca ccggatgtcg gatcgcattg
     2821 cctgcatcgg ggagacggag aacggagcgg tacggcgcat cagctttcgc gagctatacc
     2881 gcgatgtcag cagacttgcg aatgcgttga aggcgttggg gcttgaacgc ggagaccgcg
     2941 ttgcgcttac gatgccgatg gtccccgagg tggtcaccat tctgtatgcg tgccacaagc
     3001 tcgggttggt ggtggtgccg atcttcgccg gtttcggcgc gggtgctatc gccacacgcc
     3061 ttcaggactc cggtgcgcgc gttttgttca ccgcagatcg gctggagcgg cggggcaagt
     3121 tcattgagct caaagaaaaa acagatgccg cgctcgataa ctgccctacg gtatcccatg
     3181 ctgttgtgct ccgctacagg ggcggcgatg tcgcatggaa ggagcatcgc gatatctggt
     3241 gggacgactt tgtagcggga caacccgaag aatgcgaatc gctgcggctg gagggagacg
     3301 cgccggcgtt cattctgtat acttccggaa ccaccgggaa gccgaagggc accgttcaca
     3361 cgcatgccgg aagcctggcg cagatgacga aagagattta tctcgccttc gatcaccgcg
     3421 acagcgatat attcttctgg gtctccgaca tcggttggat gatgggccca tggacaatcg
     3481 tcggaaatca caattttggc ggaacgattt ttctttacga cggcgctccc gacttccctt
     3541 cgcccgatcg cttgtggcag atggtggagc gccatggcat cacgacgctg ggcatctctc
     3601 ccactgcgat acggatgctg atgaggcatg gctccgaaat cccggaacgg tatccgatga
     3661 agtccctgcg tctgctcggt tccacgggtg agccctggga cgagacgtcg tacctgtggt
     3721 atttcgaaaa ggccggcaaa ggccggctgc cgatcatcaa catctccggt ggaaccgaga
     3781 tcgccggctc ttttcttttt gtgttgccgg tgcagccgct gaaggtttgt acgctgggcg
     3841 gcccggcacc cggaatggga accgatgtgg tggacgggac agggaggtcc gtgcgcggca
     3901 gcaccggata cctggtttgc cggacgccgg caccttccat gacccgcggt atttggggcg
     3961 acccgcggcg ctacatcgag tcgtattggt cgcgctggaa aggcatgtgg tatcacggcg
     4021 actgggcccg tgtcgacgaa gacgggcact ggttcgtgca tgggagggcc gacgaatcga
     4081 tgaacgtggc cggccgcaag gtcggtccgg ccgaagtcga gcaggcgttg atgcaacatg
     4141 ccagtgtcgc cgaagccgcg gtgatcggag tgccggacga agtgaccgga gaagcaatcg
     4201 tcgcctttgt ggtgccgcgc ccgggcgcct ccgcggtgag tgaagagctg tgccgtcatg
     4261 ttgcggccgt gctcggtccg gcattccggc cgcgaagtgt cgaggtggtc tcacagctac
     4321 cgaaaacgca aagcggaaaa gttgtgcgaa ggttgatccg gcaatattac ctgggtgagg
     4381 cgccaggcga tctctcgacg gtggaaaatg cggaagcttt aaggcagttt ggcgatcagg
     4441 gcccgagccg ccggggctag acgctcacaa ccgcgcaggg cgattcgcgg ataatgccgt
     4501 aggcatttgt cctgaggcgt ccgaaaacgc cggccgcgga accgcgtccg atcaccagca
     4561 gatcggcacc tatacgctgg gcgtggtcgc agaccgtctt cggagcgtca ccggtttcga
     4621 ttttgatttt cgcttcgatg cccgcttcgg ccgcgacttc gtgaaccgct tcggtggcct
     4681 tcgtcacggc ctcggacttc caagcggcga tggcttcctc gtcgtttccg gccaccgtgc
     4741 atggcatcac atgaagcacg cggaggctgg cgtcgaaacg cttggcgatc tctcctgccc
     4801 acgtgagggt tctcttactt tgcggaccaa gatcgatcgc ggcgagaata tttctgaact
     4861 ggatagcacc taccgcgggc gcctgctcca gatgcactcc cgtccaaact ggacagtccg
     4921 cgtcgtgcag caccttggcc gtgaccgagc cgaggatgaa tcggcgaaag ggtccataac
     4981 catgtgtagg catgacgatc agtccggctt tctgttcact ggcgaacttg acgagctccg
     5041 tcgccggatc gccctcgagc agcactcgcc ggacctcgag acgcgatagt tcctcgcgaa
     5101 ggtagtcatc cagctccttc ttgacttgcg taccgcggct cgcaaaaagc tcatttaata
     5161 caggtccccc gacttccagt gcactgaatt catagtgagg cggaggcagg acgtgcacca
     5221 gaaacacttc cgagccgaag gccgtggcga gcgcttccac gtagcgcgcc gctccggcgc
     5281 ttcgttccga aaaatcgacg ggcagaatga ttttggccag ggagaacatg gggtggctcc
     5341 tccctgaaca tacagcaaaa ccacggagat gtgaaagtca gggtggcgta cgaaactacc
     5401 acaggccgga aaacgtctga ttcagtgtag ggtagtgcat ggcgggtctg tgcgacccgt
     5461 ggctgggctc agttgggtag caggtggtac cggcgtagat gtgagactca tcggcgcccg
     5521 ggtgacgttc caaaactcat tcagggaggg acagttatgg actcgcatga cttcgaattt
     5581 tcgactcccg gtactttgga ggaattgatc gagctgctct cggacccgga gaagaaaccg
     5641 cttgtgggcg atatgagcgt aattccgctc atgaaactgc ggttcgccag accttcggct
     5701 gaagccggcc tgcgtagcgt cagggggctg aacgaaatca aagaggaggg cgggagtatt
     5761 cgcattggac ccgccgcaac tcatcttcag atcgaatcct cgccgctgtt gcgcaacaga
     5821 tgccctttgc tgccggagac ggcgacgcat gccggcggca ggcaaactca cagcgccgga
     5881 acaatcggtg cgagcgtttc tcactcagac ccggtctcgg actatcccgc cgcattgctc
     5941 gctctggagg ccaagcttca cgtagtaggt aagaactccg atcgcattgt agcggcgcgg
     6001 gatttcctct cgaatacggc cgttatggct ctcgaaccgg gagaactgct gatggaaacg
     6061 acggtaccgg ttgaagactc atccaccggt gtgcactacg agagagtctc caatcccgat
     6121 tccgacttca cggtggtcgg tgtcgccgca cgcatccgga gggctgaggg caaaatcgca
     6181 atggcccggg taggggtaac cgggctcgcc gcgcgtgcgt ttcgtgcagt gaatgttgag
     6241 caggcgctcg aaggcacacc gggctctgaa accgatatca gcaatgccgc cgcgcttgtc
     6301 ggagagggtg tggatgcaag atcggatgta tacggctccg ccgaccagcg gaggcagttg
     6361 gcgagactgc acaccgaacg cgctattagc gaagcgcttc gtaaagcggc ctgacgatta
     6421 cggcgccggg accaccgcgt tccactttct tctggactcc gagatagcgg cggcgagcat
     6481 agtgctgtca aaagcggtac gggtacggcg caccagcacg acctcaacgt gagattgcag
     6541 ccactccggt tctggccgaa gctcatcgac aaggcaaacc agaacgcaag cgtcattcgt
     6601 gcggacgttg ggctccgtcg taatcacaac gggtttgttg gagggcttcc tccccggaaa
     6661 tacggcgatg ttcccgcttc cgagggcaag cacgtagtgc tccggcaata cacagacggg
     6721 cgggcgctcc acccatcgga aagcgcgcgc cgcaaagccg agtgccgggg ctgcgattac
     6781 cgggtccgta cgcagccgcc gtacggcctc ggtccacaac ttcaggaata agcgcggaga
     6841 aactacggcc ctcgcgagaa atcgaagata gttgatctcc gtgaccagcc ggagttcgtc
     6901 ctcgctatag taacgcgacg tggttgcgcg gtggtgatgt actaccgtcg cgcccgccac
     6961 aaaaaccgtc ggccagcctc gcagccacgc ccggtagccg gcgtccagat cttccacgta
     7021 agcgggtctg tacatctctc caaaacaacc caattgccgc agcttggccg tgtcatagag
     7081 agaacatccg ccgcttgcgt acaatacata gctcaagtct tcaccgggca cgggttcgtc
     7141 gcaacgcaag gggaattccg aggccggacg caacggtggc attaccgctt tgccggtctc
     7201 ctctcttcga acaccttccg gaaagaagat ctgcgccgtc gcggagaaaa gatcgggcac
     7261 gttttcgaat gccgcccgca gagcgtgaaa aaaacccgct ccaactacca tgtcgttatt
     7321 cagcaggcac acgtgcgaga aatgcgccga tgcaataccg cgattcacgg cagcggcgaa
     7381 cgagagaggt tcctgcgaga cgagaacctg aatgccgggg tatgcggagg aaagccatgt
     7441 agccgtctca tcgtcgctgc cgttgtcgac cacgatgacc tccgagggaa taccatcgag
     7501 ttcggctacg aggcgtggca gaagacgttc caacagatcc tgcccgtttc gcgacggaat
     7561 aaccaccgaa attccggatg gaaaatcggg taggcgcggc aggccggcct tgcgtggcag
     7621 cagcgctttg attgcggcaa tcaggcaacc gccggccatg gccgtccaat agcggaggat
     7681 caccgcaatt tcatatcagt tcccggctgc cggtatggaa ctgaccgtcg tgaacctcgc
     7741 gccacatctg gagagcctgt agcgcgaagg cggttgacac cggattaacg taaggaagca
     7801 tccgttcctg cttgcggccg aaccaaaacc cgccgcgcat ggaatccgcc ggctcctgta
     7861 attggaacga ggacagaagt tcagcctcct cctccgcact ggccgaatcg agcttcagca
     7921 caccgagcgc gtcagcaaac aggcgcacac gcagcagttg ggcccacacg tccgaccgtt
     7981 cgaagatcgg gcgaatttca cgcaccaggc gggcaacctt ggcgatgccg cggcgaagcg
     8041 cctccgcaca ctcctggcgc ggcgcttcgg gcagcaagcc ttccaggaaa tagcaataag
     8101 cgtgcagacg atccatgacg cgttcacgct cagtcacacc tggaaggaaa tcgctctcct
     8161 gccggagtgc aatctgcaaa gcgtgttcat aaaaaccggt gaatgacgaa tctcccgttg
     8221 cttcacctaa ttcccgccat gccagtgccg ccttgagttg ataacagccg ggatttcgag
     8281 accacgattc tccatgcgga acaggagagc cgtccggcag attcagaacc ggatggataa
     8341 cgtggccgcg cacgaaacca gcgtgcatgg catgtccggc ttccgccgcg cgttcgagaa
     8401 attcgttctg gccggtagca cgccacacgg cgagcaggcc gcgaaggatg atcccggtat
     8461 cgaagaagta tgcgaggggt tttggcggat cgctcttcga ggaggagtat tcgaatggaa
     8521 actgcgacgc ggcgctgtcc catgcatcac gcgtcaaaaa acgcgccgct ttaaggcccg
     8581 cgttcagata gtccggccag gatgtttttg ccgccaagta gaccagggta ctaacggcgt
     8641 atcccgtaat ttcagtcgat acgcgagcgt ttatgccggt atccgaatga taatagcgag
     8701 cgacgcctcc ttccggttcc tgaatgcccg agttcaaaaa ccatgcgcct gccaaacaaa
     8761 gcggatccgc aatctgcggc aaagtgtgtt ccgtcaaggg attaaactgc ttattctaac
     8821 atcggctgtc ttgaacgcca tgccgcagcg tctatactga ggaaggagtg aaccgctgat
     8881 gcgattttta gcgggcatgc tggcagtatc cgggcttgtc gctcaggatg gggcgccggc
     8941 ggggattgtg aaaggcgagc tgatcgagtg gcgggggtcc gacacgaggg gagagatcag
     9001 cctgcgagca cccgaccacc gggtttactg gtgttccttc gacgaaaaga cctacttcga
     9061 gcgagatggt aagcgtacgc tgatttcaaa ggccgccgcc cgggaaaaga tcgaacttct
     9121 ctccgaccgc ggcccggcgc catccggatg ctacgcccgc ctgatccgcg tgatggagga
     9181 aaagcatgta cctccggcgt acaaaatacg gacgatgttg cgcccggaac gccggtcatg
     9241 gcaaaccgat acgttgtttc cgaggggcaa cctgactttt gcgggtgttg tgctgtgggt
     9301 ccggccggag ttgtttgccg tgcgcatgaa tggcggtgag caaaagaaat tcgttctccg
     9361 cgaggacacg cggtacgcgg cggacgggca gaccgtgaat gcctcgatgc tggaacccaa
     9421 tactcgcgtc ttcatccgcg cgggcaagaa cctggacgat caactggagg ccttccatat
     9481 cgtgtggggc cggatcctgc tgcccggcgg cagcggacat tgagcttttc gatcggcgat
     9541 gacttacgat gcgatcataa tcgggagccg cgttgccggc gcagcgacgg ccatgcttct
     9601 ggcccgtggc gggtatcgca tcctggtggt cgatcgcgca agatttccca gcgacacaat
     9661 ttccacacac atcgtgtggc agcccggggt cgcccgtctc atcggctggg gtctcggcga
     9721 acagttgatc gccttggaag ctcctcccat tgacaccatc gaattcgatc tcggtccgtt
     9781 ctcgctcgca ggagtgccgc ctccctccgg acctgccgaa agcgcgtatg ctccccgccg
     9841 cacggtgctc gacagtatgc tcgctaatgc cgccgccgct gccggtgccg atgtccgcga
     9901 tggtttcacg gttgacgaga ttgtcttcga cggcgatcgt gttgccggga ttcgcggcca
     9961 tggcgagtcc ggtgcagtgg tcgtcgagca cgcgaacgtc gtcattggag ccgacggcgt
    10021 gcattcgatt ctggcgcgtg cggccggggc gcccgagtac aacgtgcacc caccgctgac
    10081 ctgctggtat tactcgtatt ggagtggagt gcggaacgac cgtattcgat tcttttcccg
    10141 ggaggggaga gccttcggct gtattccgac gaacgacggt ctggtttgta ttccggtcat
    10201 atggccacac gatcgtttcg ccgaggtgaa gcgcgacatc gagcgccact atctctcttc
    10261 gctcgatttg gctccggatt tcaagaagga agttttagcg ggcaagcgcg aagaacgttt
    10321 ctacgggacg gcccacattc ccaactattt ccggtcaccg tacgggcacg gctgggcgct
    10381 ggtcggagat gccgggtatc acaaagatcc gatactcgca caaggaatca gcgatgcgtt
    10441 tcgcgatgca tcgctgttgg ccgcagcact ggacgatgtc ttctccggac ggagcctttg
    10501 ggacccggcg ctgggcggtt atgaagcggc gaggaactcc gcagttgcgg caacgtatga
    10561 aatgaataca caattcgccg cattggctcc tccagcgcct gagatgcagg cgctcatggc
    10621 ggctttgcgg gggaaccagc gggacacgag tcaattcatc ggcacgatga caggaactgt
    10681 tccggtggcg gaattttatg cgccggaaaa cgtacagcgc attcggaatt acggtgacat
    10741 atccgccacc gcttgctgac gcgcgcggct ccgtttctga gccgtgaccg tgagggagcg
    10801 gccccgttga aatacgtaag cggtcagggg actttctccg tgtgggccgg ttttgccaga
    10861 tggcgataga agcccagatc cgaacccgtc aacatcttag tggcaaagat aatctggccc
    10921 gtgtggtggg cgaagtgttc cacggcgtgg tatacggctt cgagcaccgt cgtatcgtaa
    10981 ttctggattt tcgttcgctc cagcaatctt gcggccggaa gcgtgtcgat gatcgacgcg
    11041 gcttccgaga cggtaccgga cagcttcgcg gataactcgg cccggctgat accgccgtgt
    11101 gtttcaaact cgcggtcgcg ctcgcggata tcgtccaagc cgctggctcc gtatccgatc
    11161 cactggcgca cattgccgca caagtgcaga acgaggtttc caaccgcatt ctccgcgtcc
    11221 gaaccgcggg cccagatctg ggagtcgtcc agcttttcaa ggcattgttc gattctgccg
    11281 gccaattgcc tcaacttgcc ggacgagaag gcgatgaaca attcagcgtg tgttggcatt
    11341 ctcagacgat ttcctgtatc acgtgcccgt gtacttccgt caggcgttcc ttgcggccca
    11401 ggtgcatata gctgagcgcg tcctgattga gtccgagctg atggaggatg gtggtgtgca
    11461 cgtcgcggaa atgataaggt ttctcgacgg cgtgcaggcc gatttcgtcc gtggcaccga
    11521 ccacctggcc gcctttgata ccaccgcctg ccagccacat gctgaacccg aggttatgat
    11581 gatcgcgccc tttgccgcct tcggcttcgg gcgagcggcc gaactcgccg ccccacacga
    11641 ccaaagtgga atcgaggagc cctctgcgct tcaagtcctt tatcagcgcg gcgcagggtt
    11701 gatcgctctg tgcggccatg cgcagatggt tctcttcgat atccgtgtgc gcatcccatt
    11761 gcagatttcc gggtccgccg cccgagacta cgcacacgaa tcgcactcca cgctccacca
    11821 gccggcgggc gagcaggcac ctgcgcccgt aatcatccgt ggtttgccgg ccgatgccgt
    11881 acaggtcgag cgtctcctgc gattccgtcg acaagtcgaa tactgcggga gcctccgtct
    11941 gcatcttgaa tgcgagatcg taggcgttga tacgcgcttc gaattcttcg tccccagccc
    12001 gcatgttcgc ctcgttgagt tggcggagca gggacagtgt tttttttctg tcttcgagag
    12061 cgactcccga gggaagatcg aggttcagca ccggcctccg gcccggacga aacatgctgg
    12121 gttgatagat cgccggcagg aaaccgttgc cgtacatcgg ctgtccggct tcgagcgcgc
    12181 ctttggggtc cggcagaacc acataggcag gcagcgattc ggcttcagcg cccagtccat
    12241 aaatcaccca ggagcccatg ctgggaaaac cgggaatcac ccggccggtc atcaactggt
    12301 actgagcggc ggagtgcacg accatatcgc catggcagga acggatcacc gcgagatcgt
    12361 ccacgcattc ggcgatatgc ggcaggaggt ccgaaacatc gattccactg acgccatgtt
    12421 ttttgaacgt ccgcttcgtt cccatcaaca gggcattctt gctgatgaac tggtactttg
    12481 cttctccaaa agcagccggc cgcttccggc cgtgcagttt gttgagcagg ggtttggggt
    12541 cgaaggtctc caggtggctg ggaccgccgg ccatgaagag aaagatgacc gattttgcct
    12601 ttgccgtatc gggcgtatgc ggcggtttcg gcgcgagagg attcttcatc gcggcacgtg
    12661 cgtcctcgcg cgccagcatg ctcgcgaagg cgagagtccc aaatccgcaa aaagcatcgc
    12721 agagaaattc gcggcggttg cgagtgggac gaaagtgttc gggcatggct actccacgta
    12781 gaggaactcg ttcaggttca ggacggccag agcaaactcc cgcagcggcg ctcctcgcag
    12841 aaaggaaacg gcaagctgcg attcctgttt cgatggcgaa cggccaagag cgaatcggta
    12901 ggcaagctcc acctggcgtg tgcgagagat cccggcttct ttgcgcagcc gtgcggccaa
    12961 tgcttcggcc atttcgttcg ccgtccggcc gttaagcagc tcgagagctt gcggcggatg
    13021 agtgctcgat tcgcggcggg ggcaactgat ctgcaaatcc ggcgcatcga atacttccat
    13081 gaacgggagc cgcaggttac gtttcgcaat gaggtatatg ctgcggcggt gatgttcgct
    13141 tgcctcctcg gccaccgccc attgcgaggg tttatagagc agatcgatca attcctgatc
    13201 gaccggcggc ataacaccgg gtccgccttg cttgagattc aggtggcccg acacagcgag
    13261 catcgcatcg cggatctctt ccgcctccag acgccgccgg ctgtatttcc aaagcagctt
    13321 gttgtccgga tctttctccc gggcggcttt cccgatcgga gagtgtgaac tttgctggta
    13381 agcactgctg agcaggattg tgcggtgcag cggctttatg cgccagccgc cttcaacgaa
    13441 ctgattggcg agccagtcga gtaattcgcg atggctgggc aatccgccca tacggccaaa
    13501 atcgttgggc attgcggcaa tgccgcgacc gaagtgcccc tgccagatgc gattcaccat
    13561 tacacgggcc gtgagcgggt tttcgggtga ggtaatccat tcggccagcc tggttcgcgg
    13621 gttttccgga acggccgtgt attccggcac actctcttcg agcagtactc cgagcgggcg
    13681 catccccacc cgcgcaccgg ggcgcaggtg atccccgcga gagagaaggt ggaccggagt
    13741 aattctggcg ggatcgttgc tcacgctgaa gatggcggga agcggctccg gggcgcgtgc
    13801 ctccatgtct tttacctgct gctcgatctc ctccttggcg gagccttcca ggcctttcat
    13861 cgattggcgg agctttttca attgctcctg gaacgcgccg gacttctttt tccattcggc
    13921 ctgttccgct tccgacgcca ggctcacgtc tttctcgtgc gtggcggcga agtaggcctg
    13981 tatgcggtaa tagtcggcct ggcggatggg gtcaaacttg tggtcgtggc agcgggcgca
    14041 tccgagggtg acgccgagca gcgccgcccc gacggcattc gtcatctccg tcagcacttc
    14101 attccggctg ctggccactt cctggttccc cgcgttttta cgcaatggtc ccaaccgctg
    14161 aaagccagcc gcgatctgca acgcctggtt gccgggttca agttcgtctc ccgccagttg
    14221 ctccttgacg aaacggtcat agggtttgtc ttcattgaaa gagcgaatga cgtagtccct
    14281 atatcgccat gcgccgttcc gatgtgtgtc gtactcgaag ccgtccgttt cggcataacg
    14341 aaccacatcc agccatcgtt gcccccaacg ctcaccgtaa tggggactgg cgaggagccg
    14401 gtccaccact ctttcccagg cgccgggaag tttgtccgag gcgaagttct ccacctcagc
    14461 gggagcagga gggagtccga tcaggtcgaa atagactcgc cggatcagta ttcggcgcgc
    14521 ggccgggggt gcgggctcca agccttcttt gcggagcttc tccagcacga aagcatcaat
    14581 cggcgtcttc acccaggctt tcccggcagc gtcggagaaa acgggtacct cggggttctt
    14641 gcgcttctgg aacgcccagt ggcggcgctc cgcggccgtg taggagccga gcggcgcata
    14701 ttcatcggac gctggaagcg ggtaaacggc cgctacgcac aacagaagct gaaaaaagcc
    14761 aaaaaccgct cgctggcgcg cgcggctcgg aaacgcgttg acgtgtttgc aggtacttgc
    14821 cgagccgtga ccgcaaggga acggtggtcc aacggccctt ttcggcagac tgctaaaggt
    14881 gagcatccca gttgggatgt attatatagc accgccagcc ggcgcgggcg gttcagattc
    14941 ggaccagttc gtcctgcgat tcgcccgtca cgatggcttc ctcatcgcct acgaacacat
    15001 tcacttcgga ccggacgccg aactcgggaa gatagatgcc gggctcaatg gaaaaacacg
    15061 tccacggaat gatgcgccgc tcgtcgtgag tttcgagatt atccatattg gcgccggttc
    15121 cgtggacatc cattccaatg gaatgacccg tgcgatgaaa gaagtattcg gcgtagctct
    15181 tttcccggat aaacccacgg gcggcatcgt ccacttcgaa gccgcgcagc ggctgcctgg
    15241 tttggacggc gcgctgcacg gtttcgacag cccgcaggcg cgcccggcgg acgatatcga
    15301 agatctcgcg catcttctcc ggcggttcgg acccgcagta ccccgtccag gtgatgtcgt
    15361 agtaaacgct gcccggcgtg tccagcttgg cccacatgtc caggagcaca aagtcaccgg
    15421 agcggatctc ctggggagct tccgccgtcg gctcgtaatg cgggttggaa cagttcgcat
    15481 tgacggcaac gatagggccg tgatccgtca caaggccgtt ggcggcgaat tcccgcaaga
    15541 taaattgctt gatatcccac tcggtgatgc ggctgttcgc acggattcgc ccgccgatct
    15601 cctggaacgc cgcggcgcga atccggtcga tacgccgtcc ggcctcgaga tgcgattcca
    15661 gttgattgct gttccagctc gcctcgaagc gctgaaccag gtcggcggac gtaaccacct
    15721 ccaccccggt cgcgcgcacc agttcgagcg tgccgccatc caccagcgat acgtaaggca
    15781 cggcgcaatc gggagaatac tgcatggcga cacggcggca gccttccagc agatacgtca
    15841 atcccgcggc ctgttgttgc cagctcgcat agggccgttt ctcgcccgga agactgtcga
    15901 gcatcgagct ttcgatccgg tgcaccaggg cgcgcggctc gccattcgcg gggaccaggt
    15961 aataccagcg gcgagtgacg tggcgaacag gcgtgaatcc caggatgcga taggctaatt
    16021 cgtcccgctg gtgatgatcg aagaacagcc agccgtcaat ctgttcctgc cgcaacgctt
    16081 cttgaatttc agtgagtctc atgttgaaaa gggacgggct ctgaaaaaaa cgggtcgact
    16141 ttttccggtg gcggtggagg cgtgagcagg ctgaccaata tcaggctggc caccgatgca
    16201 cccagggccg gatagatcgc atccagtgtg ctgatctcga aaaggttcca gaccaccgtg
    16261 atgatcgtgc ccagaaggat ggaggagatg gc
//
LOCUS       DASTMK010000348         6571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_559894, whole genome shotgun sequence.
ACCESSION   DASTMK010000348 DASTMK010000000
VERSION     DASTMK010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559894"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VFQ79_15285"
     CDS             complement(<1..161)
                     /locus_tag="VFQ79_15285"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEU0141085.1"
                     /translation="MAYDDLGDFIRTLEKHRELKRIPFEVDPVLEITEFADRSVKNGG
                     PALLFEKAKG"
     gene            246..980
                     /gene="fabG"
                     /locus_tag="VFQ79_15290"
     CDS             246..980
                     /gene="fabG"
                     /locus_tag="VFQ79_15290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU0141086.1"
                     /translation="MHNRVAFVTGSSRGIGRAIALTLCREGCRIVVASPEVEKNEEVA
                     SEIRNSGGQAMTVNLDLTSTDSIKEGFAAAQREWGRVDILVNNAGITKDALAMRMKPQ
                     DWDIVLRINLSGAFFAIQQALPGMVRERWGRIVNIASVVGRSGNPGQANYVASKAGLI
                     GLTKTVAQEVGSRNITVNAVAPGFIETDMTQVLPQEVKDRMLAQIPLKRSGKPEDVAN
                     VVKFLASDDAAYITGQVIDVNGGMYM"
     gene            complement(1063..1857)
                     /locus_tag="VFQ79_15295"
     CDS             complement(1063..1857)
                     /locus_tag="VFQ79_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEU0141087.1"
                     /translation="MHLHSRFILCSVPLLALGLVYAQREFRSYSFEDEAVPLPADAGE
                     KTEWAFARLQYSSAFGRWGRGSWATDYPKADRQFVLGVRRLTRIHTRSVEQVVNLAQD
                     SEEIYNWPWIYGVGVGRWGLSDQQAARLRDYLLRGGFLMVDDFHGTREWEIFLASMSR
                     VFPDRPIEEIEPGDPIFHVLYDLERMMQVPGIHTIYSGRTFERDGIEPRWRAIYDDRR
                     RIMVAICFNQDNGDAWEHADDPRYPEAAASQAYRMAINYIIYAMTH"
     gene            complement(1935..2828)
                     /locus_tag="VFQ79_15300"
     CDS             complement(1935..2828)
                     /locus_tag="VFQ79_15300"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEU0141088.1"
                     /translation="MRKFAFAALMAVSPAFLFAETAQERLQESTKVFNEIMQSRDSAI
                     PEDLLEKAHCAVIVPGLKTAAFGVGGKYGRGFAICRRSGRAGWGAPGAVRVEGGSFGF
                     QIGGSDTDVVMLVMNERGMERLKESKFTLGANASVAAGPVGRTATANTDAYMTAEILS
                     WSRSRGVFAGVALEGATLRNDLDENQELYGKRLTNEQILLSNMAPPAAAKPLVASLNK
                     YSRFEEGTSSADRKVNRKVNKAERTAEKAGDKAERTAEKAGDKIERTADKAADKLEDA
                     ADKTADKTRKAGDKVEDKLDR"
     gene            3003..3770
                     /locus_tag="VFQ79_15305"
     CDS             3003..3770
                     /locus_tag="VFQ79_15305"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0141089.1"
                     /translation="MKLGAVTYNLLKDLDVEGIIKVLEAARFEAVELRTTHKHGVEPS
                     LSADERRRVRERVQNSKVRLLSYGTTCEFHSPDAAVRRKNVEIAKQFIDLAHDTAAWG
                     IKVRPNALPKEVPVETTIRNIGAGLREAGEYGSGKGVEIWLEVHGRETSQPKICADIM
                     KAANHRNVGLCWNSNENDIVNGSVKPSFDLLRPWIKHVHINELANGTYPWRELFMLLR
                     QSGYDRYTLCEAADNPQTERFLRWYHALWTEFNRPCS"
     gene            3761..6148
                     /locus_tag="VFQ79_15310"
     CDS             3761..6148
                     /locus_tag="VFQ79_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HEU0141090.1"
                     /translation="MLLKPGIVFLLTLQALVAAVPAPHAFFGHAIGADRTSLEWAKVA
                     AYFQLLEKNSDRIRVSDVGKSTLGRPMIAAFISSPETIASLDEYRLIQASLADPRRTP
                     EAEAEKLIATGKTVVMITCSIHSTEVASTHTAIEFAYKLLTEDTPKQRAILDNTILIL
                     VPSLNPDGVDIVTNWYRKTLGTKFEGTSPPELYHKYVGHDNNRDWYIFSQAETRHVIS
                     QLHNRWHPQIVYDVHQQSPYASRMFVPPWMDPIDPNIDPIIAQQCNAIGMGMAADLTA
                     AGKTGVVVNALYDFWTPGRHYQAYHGGLRILSESASARLSSPIVVRPEQISERGHGYN
                     PRESAWNHLQPWLGGEWRLRDIIDYQAIAMESLLYQAAVRRPDLLRAFYRIGQRATER
                     NRPQAFAVPAVQRDPGASKKLLETLSFGMVEIERARQRFESKGKAYPAGTYVIRMQQP
                     YSSFAKTLLERQNYPDLRLYPGGPPKRPYDVTAHTLPLLMGVEVDTLETPVSAPLDPA
                     GEFSFRLPRPSNGMAASDTGTWNKVNQAWNKSTPVWRDLTTGDFHISGGEGRRSVKRP
                     RVGLYRSYVPSMDEGWTRWLLEEFGFAYTSVDNSRIAKGSLRRDFDVLLFPDQSSSTI
                     ANGYREGDMPAGYTGGLGTQGADALKAFAAGGGTLIFLNDSCEYAVKQLALPVKEVLK
                     GLPASEFYSPGSLLNVRLDTKHPLAYGLPPEIAIWSQGSPAWTLTGDSREVAQYPREK
                     ILASGWLLGEKHIAGKSAIADVPLGKGRVILFGMRPQYRAQSYQAFKLLFNALLY"
     gene            complement(6161..>6571)
                     /locus_tag="VFQ79_15315"
     CDS             complement(6161..>6571)
                     /locus_tag="VFQ79_15315"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0141091.1"
                     /translation="NAHEGSLSREDYARFSEPFDKMVLEAVKTAPLNILHIHGDKIYL
                     DRFYRGWLAAAINYSIHGSGIPFEEARRHYTGVLMGGVDEVHFRKLSQDQMKAQWMAA
                     QKAAGPKYILAPGCSVPNDTADEELLRLTRAVGA"
BASE COUNT         1413 a         1969 c         1874 g         1315 t
ORIGIN      
        1 cccttcgctt tctcaaacag cagcgccggc cctccgttct tcacggagcg gtccgcgaat
       61 tccgtgattt cgagcaccgg atccacttcg aaaggaattc tcttgagctc gcgatgcttc
      121 tcgagcgtgc ggatgaaatc acccaaatcg tcgtaagcca tgatgtgtgc ggaaaacttc
      181 tttttactat gatggagctt tgccggcaaa accggtactc agccagttca aattccagga
      241 gaatcatgca caatcgagtc gcattcgtca caggttcgag ccgcggcatt ggacgcgcca
      301 tcgcactgac gctatgccgc gagggatgcc ggatcgtcgt cgcctcgccg gaagtggaaa
      361 agaacgagga agtcgcatcc gaaatccgca acagtggcgg ccaggcgatg actgtaaacc
      421 tggatctgac ctcgacggac tccatcaaag aaggctttgc cgccgcacag cgggaatggg
      481 ggcgcgtgga cattttggtg aataacgcag gcatcaccaa agacgccctg gcgatgcgaa
      541 tgaagccgca ggactgggac atcgttctgc gaatcaatct gagcggcgca ttttttgcca
      601 tccagcaggc actgcccgga atggttcgcg agcgatgggg ccgcatcgtg aatatcgcgt
      661 ctgttgtggg gcggtccgga aaccccggac aggcgaacta tgttgcatcg aaggccggtc
      721 tcatcggatt gaccaagacc gtcgcgcagg aagtgggaag ccgcaacata acggtcaatg
      781 ccgtggcgcc ggggttcatt gaaaccgata tgacccaggt gctgccacag gaggtgaaag
      841 accggatgct ggctcagatc cccctgaaac ggtcgggaaa accggaagac gtggcaaacg
      901 ttgtaaaatt tctcgccagc gacgatgccg cctatattac cgggcaggtg attgacgtca
      961 acggcgggat gtatatgtga atcccgcgac gccgctccga aacggagccg cgcgcgtaag
     1021 caagcgggtg gcggtgtcac tgcaattccg aatagaagtt actcagtgag tcatggcgta
     1081 gatgatgtag ttgatcgcca tgcggtaggc ctgcgacgcg gcggcttccg ggtacctcgg
     1141 atcgtcggca tgctcccagg catcgccgtt atcctgattg aagcagatgg cgaccataat
     1201 acggcggcgg tcatcgtaaa tcgcccgcca gcgaggttcg atgccgtcac gctcgaacgt
     1261 gcggccgctg taaatggtgt gtatgcccgg cacctgcatc atccgctcca ggtcataaag
     1321 gacatggaag atggggtcgc ccggctcaat ttcctcgatc ggacggtccg gaaaaacacg
     1381 gctcatgctg gccaggaaaa tctcccattc gcgcgtgccg tgaaaatcgt ccaccatgag
     1441 gaacccaccc cgcaagagat agtcgcggag ccgggccgcc tgttgatcgg agagacccca
     1501 gcggcccacc ccaactccat aaatccaggg ccagttataa atttcctcgc tgtcctgcgc
     1561 gaggttgacg acctgctcca cggatcgtgt atggatgcgt gtcagacggc gaacccccag
     1621 aacaaattgc cggtcagcct tgggataatc cgtcgcccag cttccgcgcc cccatcgccc
     1681 gaaagccgag gagtactgta gccgtgcgaa ggcccactcg gtcttctcac cggcatcggc
     1741 gggaagcgga acagcttcgt cttcgaagga atagctgcgg aattcccgtt gggcgtagac
     1801 taagcccaat gcgagcaagg ggacagaaca caatataaac cgggaatgga ggtgcatccg
     1861 gaacctcctg acgcactcag aataacacgc gccatgccgc ggaagccgaa agcctccttc
     1921 ccgtttagga accactagcg gtccagttta tcctccacct tgtcgccggc tttccttgtt
     1981 ttgtcggctg tcttgtccgc agcgtcttcg agtttgtccg ctgccttgtc cgccgtacgt
     2041 tctatcttgt ctcccgcctt ctcggcagtg cgttctgctt tatcacccgc tttttctgcg
     2101 gtacgttctg ctttgtttac cttccggttc accttgcggt cggcgctcga agtaccttcc
     2161 tcgaaacggg aatacttgtt caaggacgcg accaaaggct tcgcggcggc cggcggagcc
     2221 atattggaaa gcagaatctg ctcattggtc agacgctttc catagagctc ttgattttcg
     2281 tcaagatcgt tccgcagggt agcgccttcc aaggccacac ccgcgaaaac tccccgcgac
     2341 cgggaccagg acagaatttc ggctgtcata taggcatccg tgttagcggt cgcggtccgg
     2401 cctactggcc cagccgcaac cgaggcattg gcgccaagtg tgaacttgct ttccttcagc
     2461 cgttccattc cccgctcatt catcacgagc attacgacgt cggtatcaga ccctccgatc
     2521 tgaaatccga aactgcctcc ctccacccgc accgcgccag gggctcccca gcctgcacgg
     2581 cctgaccggc ggcaaatggc aaaaccacgg ccatacttcc cgcctacacc aaatgcggca
     2641 gttttcaacc caggaacaat cacggcgcag tgcgcctttt cgagaagatc ctccggaatc
     2701 gcgctatcgc gcgactgcat aatctcgtta aagactttgg tcgattcctg caagcgctcc
     2761 tgcgcggtct ctgcaaacag gaacgccggg cttacagcca tcaatgcagc gaatgcaaat
     2821 tttcgcataa tccctcccct cgaaaactca tgcaaccaac atgccgaaac gaaggagcat
     2881 taccttcaat agtttaggtg ggcatctccc agaacgtggc tgtctttttt tccgtgtgga
     2941 gagagccttc catctcagcg cgctacaatg acaattcaat ttctcacaga ggagccaggc
     3001 ggatgaaact cggcgcggtg acatacaact tgctgaagga tctggacgtg gaaggcatca
     3061 tcaaagtcct ggaggcggct cgctttgaag ctgttgaact ccgcacgact cataagcatg
     3121 gcgtagaacc ctcgctttcc gcagacgaac gccggcgtgt tcgcgaaaga gttcagaaca
     3181 gcaaggtgcg cctgctcagc tacggcacca cgtgcgaatt tcactctccc gatgccgccg
     3241 tacgccggaa gaacgtggaa atcgcgaaac agttcatcga cctcgcccac gataccgcgg
     3301 cctggggcat caaggtgcgc cccaatgctc tgcccaaaga agtgccggtt gaaaccacta
     3361 ttcgaaatat cggcgccggc ttgcgggaag cgggagagta cggcagcggg aaaggcgtgg
     3421 agatctggct cgaagtgcac gggcgggaga cctcgcaacc gaagatttgc gcggacatta
     3481 tgaaagccgc caaccatcgg aacgtaggcc tctgctggaa ctccaatgag aacgacatcg
     3541 tcaacggttc cgtgaagccg agctttgatc tgttgcggcc ctggatcaag cacgtccaca
     3601 taaacgagct cgccaacggc acgtatccct ggcgagagct gttcatgctg ctgcggcaat
     3661 ccgggtacga ccgctacacc ctttgcgaag cggctgacaa cccgcaaacg gagcgctttc
     3721 tacgctggta tcatgcgctc tggacggagt tcaaccggcc gtgctcctga agcccggcat
     3781 cgtcttcctg ctgactctgc aggcgcttgt ggcagccgtt ccggcgccac acgcgttctt
     3841 cggacatgcc atcggcgcgg accggacatc gctcgagtgg gccaaggtag ccgcttattt
     3901 ccagttgctc gagaagaaca gcgaccggat tcgtgtcagc gacgtgggaa agagcaccct
     3961 cggacgcccg atgatcgcgg cgttcatctc aagtccggaa actatcgcca gcctggacga
     4021 gtaccgcctc attcaggcct cgctagcgga cccgaggcgc actccggagg cggaagccga
     4081 aaagctgatt gcgaccggca aaacggtggt gatgattact tgctctatcc actccacaga
     4141 agtcgcttcg acgcatacgg cgatcgagtt cgcctacaag ctgcttacag aggacactcc
     4201 gaaacagcgc gcgattctcg ataacacgat tctgattctt gtgccgtctt tgaacccgga
     4261 tggagtcgac atcgtcacaa actggtaccg gaaaacgcta ggaaccaagt ttgaagggac
     4321 gtcccctccg gagctttacc acaagtacgt cgggcacgat aacaaccgcg actggtatat
     4381 tttttcgcag gcggaaacca ggcatgtaat ttcgcagttg cacaatcgct ggcatccaca
     4441 gattgtctac gacgttcacc agcagagccc ttacgcctca cgcatgttcg ttccgccctg
     4501 gatggacccg attgacccga acatcgaccc tatcatcgcc caacagtgca atgccattgg
     4561 aatgggcatg gcggcggacc tgactgccgc ggggaaaaca ggcgttgtcg tgaatgctct
     4621 gtacgatttc tggactccgg ggcgccacta ccaggcatac cacggcggtt tgaggatctt
     4681 gagcgaatcg gccagcgcgc ggctttcaag tcccatcgtc gtccgcccgg agcagatctc
     4741 ggaaagaggg cacggataca atccgcgcga gagcgcctgg aaccatctgc aaccatggct
     4801 gggcggcgag tggcgtctgc gcgacattat cgattaccag gcgatcgcga tggagtcctt
     4861 gctgtatcag gcggcggtcc ggcgcccgga cctgctccgt gctttctatc gaatcggcca
     4921 gagggccacg gagagaaaca ggccgcaagc cttcgccgtt cccgcggttc agcgcgaccc
     4981 gggcgcgtct aagaaactac tggagacgtt gagcttcggc atggtggaaa tcgagcgcgc
     5041 gcggcagcgc ttcgaatcga aaggcaaggc atatccggcc ggcacctacg tgatacgaat
     5101 gcagcagccg tatagctcgt tcgccaagac cttgcttgaa cggcagaact atcctgacct
     5161 gcgactctac cccgggggcc cgccgaaacg gccctacgat gtaacggcac acacgttacc
     5221 gctattgatg ggcgtggaag tcgatacgtt ggaaactccc gtcagcgcac cactcgatcc
     5281 ggcgggtgag ttttccttcc ggcttccgag gccgtccaac ggcatggccg cttccgatac
     5341 cggcacctgg aataaagtga accaggcctg gaataaaagc acaccagtgt ggcgcgatct
     5401 tacgacgggt gatttccaca taagcggtgg cgagggccgt agatcggtga agcgcccgcg
     5461 ggttggcctg tacaggagct acgtgccgtc gatggacgag gggtggacgc gatggctgct
     5521 ggaggaattc ggctttgcct acacgtccgt cgacaacagc cggatcgcga aaggctctct
     5581 gcggcgcgac ttcgacgtgc tcctatttcc cgatcagtcg agctctacga ttgcgaacgg
     5641 ctaccgcgaa ggcgatatgc ccgccgggta tacgggtgga ttaggaacgc aaggtgcgga
     5701 cgcgctcaag gcctttgccg ctggtggagg cacccttatt tttctgaacg actcttgcga
     5761 gtacgcggtg aagcagctcg cacttcccgt gaaagaggtc ttgaagggtt tgccggcttc
     5821 cgaattctat tcgccgggat cgctgctgaa cgtgcgcctg gatacgaagc acccgctcgc
     5881 ctatgggctt ccgccggaga tcgccatctg gtcgcagggc agtcccgcat ggacgcttac
     5941 cggcgattca cgtgaagtgg cgcagtaccc gcgcgaaaaa atcctggcct cgggatggct
     6001 gcttggagaa aagcacatcg ccggaaaatc ggctatcgcc gatgttccgc tcggcaaagg
     6061 tcgcgtaatc cttttcggta tgcggccaca gtaccgcgcc cagagttacc aggcgttcaa
     6121 actgctgttt aacgctttgt tatattaggg cgccgcacct ttacgcgcca accgcgcgcg
     6181 tcagccggag cagttcttca tcggcggtgt cgttcggcac cgaacacccg ggcgccagaa
     6241 tatatttcgg cccggcggct ttttgcgccg ccatccactg ggctttcatc tggtcctgac
     6301 tcagcttccg gaagtgcact tcatccacgc cgcccatcag cacgcccgtg tagtgccggc
     6361 gcgcctcttc aaaaggaatc ccgctgccat ggatggaata gttgatggcc gccgccagcc
     6421 agcccctgta aaaccgatcc agatagattt tgtcgccgtg aatgtgcaga atgttgagcg
     6481 gcgcggtctt caccgcttcg agcaccatct tgtcgaacgg ctcgctgaac cgggcataat
     6541 cctcgcgcga aagactgcct tcgtgcgcgt t
//
LOCUS       DASTMK010000349        11747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_625151, whole genome shotgun sequence.
ACCESSION   DASTMK010000349 DASTMK010000000
VERSION     DASTMK010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625151"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1558
                     /locus_tag="VFQ79_15320"
     CDS             239..1558
                     /locus_tag="VFQ79_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141092.1"
                     /translation="MSSMEPRATANRRDFCKTAGAAALTTSLFTGSIKGANDKITVGY
                     IGMGRMGVANLGISMKNPAIQPVAVCDVYQPHLEKAEALARRDGHQAKGYRDFREIIA
                     DKSIDVVCISTPEHWHPYMTIEACKAGKDVYVEKPLCVYVEEGQKMVEAARKYKRVVQ
                     AGTMQRSGVHFQKAVEIVKSGELGDITFCRTWQAGLTKQEGWGNPPDSEPPAGLDWDM
                     WLGPAPKRPFNANRWGVRPDAFSTFRYFWDYAGGAMTDWGVHLLDITQWAFDEVMPKS
                     VDAMGSKFYVKDNVETPDTMLVTYMYPDFIGSYESRTANPMPMHANERYGTSFHGTKG
                     TLIVNRGGYEVFLTGERGEQSRGDHTRQQMNEPHWQNFVECIRSRQKPISDIETCYRT
                     SATCILSNLAMRLKMRLDWDDSKQTVLQDAAKPYLKAKYRAPWKLEV"
     gene            complement(1569..2444)
                     /locus_tag="VFQ79_15325"
     CDS             complement(1569..2444)
                     /locus_tag="VFQ79_15325"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0141093.1"
                     /translation="MNLTRRDFVALAAGAPLAASQGSGKPSLCIFSKHMANLNYEQLG
                     KTSREMGFDGVDLTVRAKSHVLPERAEEDMPRAVEAIRSHGVTVPMITTNILRPDEPT
                     TRPILSTASRLKIPLYKPGYWLYRTPAVLDTIQQVKKATEGLVQMGKEYGIVSGFHNH
                     SGNYVGTAIWDLRDIIKDLDPAWIGYYFDPAHATIEGGSFNWILSARIALPRLKMVAL
                     KDFYWEKSGGRWRVKWCPLGEGMVDWGRFFTMLAEARFTGPISLHVEYEPADEPAAIA
                     KDLEFMKAQVAKAYK"
     gene            2513..3355
                     /locus_tag="VFQ79_15330"
     CDS             2513..3355
                     /locus_tag="VFQ79_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU0141094.1"
                     /translation="MTRRIAGLLLMSTWMGMAAGPIAVHGHRGARTVLPENTIPAFEY
                     AIEHRADFIELDLAVTKDNVLVVSHDPIVNETICKGPRKQAVIREMTAAELREWDCGA
                     IANPEFPRQKTVPGTRIPTFDEVLALAPRGNFGFTIELKSNPQRPQFAPAPDEFARLV
                     VDAIRRHKLEDRSIVQSFDFRTLRATRKIAPNIRLAALYSGPPKDFPLIAQEAQADIV
                     APNFKLITPEQIQAAHQAGKQVVPWTPNEPAEWDRLIAAGVDAIITDDPAGLIAHLDR
                     KGLR"
     gene            complement(3425..4399)
                     /locus_tag="VFQ79_15335"
     CDS             complement(3425..4399)
                     /locus_tag="VFQ79_15335"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU0141095.1"
                     /translation="MPGLLAGQAPRIGIIDFYGLKKVSEESVRKVLTVKEGDPLPPSK
                     SDVEEAIEKLPGVVLASLEAVCCEAGKAILYVGIEEKGASHFALRSFPEGDVTLPTDI
                     LDTYQSFLDCVQEAVRQGETGEDLTAGHSMMQNGAARRVQEKFLTIAAAQLSTIRDTL
                     LNSRDEQHRAIAAYLIGYAPDKQAVVNDLQTAMQDPDETVRSNAMRSLGAMMVLSQRK
                     PDLGLKVSPTWFVEMLNSILWNDRHSAAVALVNLTESRDPSVLDSIRERGLDSTAGMA
                     RWKHLPHSLPAYILLGRVAGFPEKEIQDSWKKGDQEAFITRALKQIRK"
     gene            complement(4460..4789)
                     /locus_tag="VFQ79_15340"
     CDS             complement(4460..4789)
                     /locus_tag="VFQ79_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141096.1"
                     /translation="MAAALCYLLGFITGIIFLVIEPYNRNKTVRFHAFQSIFVSIGVF
                     VISVAIGILAMMFYAISFWLGSLVGMLQLVLGLGFFILWLYMMWKAYQNERVVLPVVG
                     PLAEKQA"
     gene            5104..6216
                     /locus_tag="VFQ79_15345"
     CDS             5104..6216
                     /locus_tag="VFQ79_15345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141097.1"
                     /translation="MNYFDRRHFLFASAAAAQAALAQQTGDRIGTAMIGTGNRGSHVL
                     RGVLEQPNARVVALCDIKPDRLDAAASSAAKDNPKTYTDWRRVIDNKEVEAVFIATPP
                     HLHAEMAIAALKAGKHVYCEKPIGVTADQVKQLVRAAKGVNKVFVSGQQLRSHQQLKE
                     AVSKIHQGELGELLMVKAQRHATTDLNHEGSSADWYFDVTKSGGYLIEQSVHNLDACN
                     WVIGAHPTRATGFGGVQLYKNDPPGRTIMDNASLTFEYPSGLKLSFTQNVFHPSGMPC
                     GGQYIHVFGSKASVDLLYAMNEYPLPKGEVKVIAEKRQEPQHAHTTAFYDCIVNGAEN
                     PADITVGATAALTSILGHEAMVRERVVNWRDLGVEV"
     gene            complement(6265..6462)
                     /locus_tag="VFQ79_15350"
     CDS             complement(6265..6462)
                     /locus_tag="VFQ79_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141098.1"
                     /translation="MAHLEGKLPALTCLLAFALLLSAQDTRPVIGEQHYRIYHGDGRP
                     ARDLFIPGCDPQGHEKLGNEC"
     gene            complement(6464..6715)
                     /locus_tag="VFQ79_15355"
     CDS             complement(6464..6715)
                     /locus_tag="VFQ79_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141099.1"
                     /translation="MQNNGCILAEVPGFGRIYDCGACGNLHLSVGPVSITLTTDAYMQ
                     LVALLNTSASNFELWMQAKDIGGSMLDQEHDTPRIEGEP"
     gene            complement(6718..8283)
                     /locus_tag="VFQ79_15360"
     CDS             complement(6718..8283)
                     /locus_tag="VFQ79_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141100.1"
                     /translation="MKSRFFQFFPLLTCVSLAHAHFTFVVPEPDGTKAKMIMSETLTP
                     EPGVDVAIIGGTKLYVRTVDGSDTPLTLVKEANAYEVSLAGAGTRVVHGFTDLGIMQR
                     GSGKPHVLLYYPKTILGDAFDPKTVVGGETPVEIVPIGKPGDLKLKLVGRGKPVPHAE
                     ITVILPDATQKIVKTDESGLTETFSQTGRFGAWARFWESVPGERNGKKYEELRHYATL
                     VFDAAAASVFATMPEAASSFGAVESDGWLYVYGGHIAATHSYSTESVSGRFSRLNLVS
                     RQWEDLPPGPGLQGMNLTAHNGRIYRIGGMAPRNKPGEPADNRSTSGCARYDPATRKW
                     EALPPLPQARSSHDVVVVGNQMIVTGGWTLAGSKQKWADTLVTMNLSEPKLEWKSAAQ
                     PFRRRALIAVAFQEKLYVIGGINDKDQIEPKVSIYDPKAGTWSEGPALPEGVMAFAPA
                     ATVHNGSLYVSVSDGTLFRLDGSRWEKAGKATGRVAHRLVSYKDGILVIGGAARRKNF
                     DLIEPVSIQRSVE"
     gene            complement(8325..10586)
                     /locus_tag="VFQ79_15365"
     CDS             complement(8325..10586)
                     /locus_tag="VFQ79_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141101.1"
                     /translation="MSGYRKLSAALAALTIAGAALSQAPTNPPVLRISVTDAAALPLV
                     GARITVRSTLGAFSFDQATGQDGAVELRPPLSGRYLVQIRAQGFADESRYVDWRNETV
                     SMDVRLSVGGIREQITVTSGSRVEELQQDSPVKVEAVTRQSMITTGYERLSDVLSEIP
                     GVVTRSGSSGSVSTEQIRGISARQVAVLQDGLPMIGARGIKSGNLNLNRQSTGRLERV
                     EVAKGAASTLFGSDAIGGVINMISREPTQKLQGNLNFSGGSLGIFDGRGDIGTRYKNL
                     SIFLDLEHHRQDSYSLLPNSLFTVGPDFRRDDLLFKTRYAFTPRFTLGFSANAYRNRE
                     EGRNASETGPVLGLYNDSVQNYALVGDIVPASRTTMQLRAYSARYDENSRLDSLGAPA
                     PPALANLNQRYKRLDGSISHQLGSRNLLQGGYEWVQDNYKGANRLVGDNDGRQVTSND
                     VWLQDKFHLTRRATLDAGGRVTSHSLFGTWAVPQVGLVVRLSDHFTARGSFGRGFRAP
                     DLGQLYFRFANPASFYQVIGNPNLEPETSRSFSTGVDFRARRFRAGVSLFRNDVRNLI
                     DSVNIGTPRTSEELTALLNAYGIPASFMPLLNRQTFIYQNFGRIYAQGFELDAAHSIT
                     RQFQVSAAYTYLDARDTDTKLGLPQRHKHQGYVRTEYLNPRLGLFANVRGMFFSKWLL
                     NPAAGTRAFGYSVWDFYVSKNLVHGAQAYFSIDNFAGSRDQKLSLPTPTFDRPDYGRQ
                     YRVGLRWRFGGVE"
     gene            complement(10988..>11747)
                     /locus_tag="VFQ79_15370"
     CDS             complement(10988..>11747)
                     /locus_tag="VFQ79_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141102.1"
                     /translation="WSPFLAGGRPTTVCIGEPFFIHFPDAHAFVRLPAAYDWEQARTS
                     PSINKLLESLRETDPIPWHSFTGVGEARAAFLLGKLLGTVREDVLVTASSRLSWDQIV
                     AHNVIFLGPPKYNPQLRRIPISQEFVFEDNGIRNLHPEAGEPAYFPDEIEKGSSTRDT
                     GETHGLISRLPGPHRNAQMLILSGNWTVGTMGATQYMTQPQYARDLVSRIRLPSGQLP
                     EHYQVVVKVSFNAGVPVRVSYLNHRVLQADQNRH"
BASE COUNT         2524 a         3459 c         3405 g         2359 t
ORIGIN      
        1 cgaagtaaaa agagagttcc cacaggcaat gcagcggtac atgcccgctt cgtagtgctt
       61 gttgtacttg ccggtgaaag gcgcttccgt agctttgtta cgggtgacct cgtaggaaag
      121 cggcggcaat tcccgtttcc attcttcttc gcttttttgt ggtttatctg gcatgccgag
      181 ttcccagtcc cctcctaatt gctatggtaa aacagggttg taaaggaaac ggacccatat
      241 gtcatcaatg gagccgcggg cgaccgcaaa ccgtagggat ttctgcaaga cggccggagc
      301 cgccgctctg acgacgtctc ttttcacggg aagcatcaag ggcgcgaacg acaagataac
      361 ggttggatac atcgggatgg gccgcatggg cgtggcgaat ctcggcattt cgatgaagaa
      421 ccccgccatc caacctgtcg cggtctgcga cgtgtaccaa ccgcacttgg aaaaggcgga
      481 agcgctggca cgccgcgacg gacaccaggc caaaggctac cgcgattttc gcgagatcat
      541 cgcggacaag tccatcgacg tcgtttgcat ttctactccg gaacactggc acccttacat
      601 gaccatagag gcgtgtaagg cgggcaagga tgtatatgtc gagaagccgc tgtgtgttta
      661 cgtggaagag ggacagaaga tggtggaggc cgcgcgcaag tacaagcggg tggttcaggc
      721 cggcaccatg cagcgctccg gcgtgcattt tcagaaagcc gtcgagatcg ttaaaagcgg
      781 cgagttaggc gacatcacgt tctgccgcac ctggcaggcc ggactcacga aacaggaagg
      841 gtggggaaat ccgccggaca gcgagcctcc cgccggtctg gattgggaca tgtggctcgg
      901 ccccgctccc aagcgtccgt tcaatgcgaa ccgctggggc gtgcggcctg acgcattctc
      961 cacgttccgt tatttttggg actatgccgg cggcgcgatg acggactggg gcgtccatct
     1021 gctcgacatc acacagtggg cgttcgacga agtaatgccg aaatcggtgg atgcgatggg
     1081 ttcgaaattc tacgtgaaag ataacgtgga gacccccgac accatgctgg tcacctacat
     1141 gtatccggac ttcatcggct cttacgaaag ccgcaccgcg aatcccatgc cgatgcacgc
     1201 gaacgagcgc tacggcacgt cgttccatgg aacaaaaggg actctgattg tgaatcgcgg
     1261 cggatacgag gttttcctta cgggtgagcg cggcgagcag tcccgcggcg accataccag
     1321 gcagcagatg aacgagccgc actggcagaa cttcgtcgag tgtatccgca gccgccagaa
     1381 gcccatcagc gatatcgaaa cctgctaccg gacctcggca acgtgcattc tttccaacct
     1441 ggcgatgcgg ctgaaaatgc gactggattg ggacgacagc aagcaaaccg tattacagga
     1501 cgcagccaag ccttacctca aagcgaaata ccgcgcaccc tggaagctgg aagtctgacg
     1561 aggccgcact acttatacgc cttcgccacc tgcgccttca tgaactccag atccttggcg
     1621 atggcggcgg gctcgtccgc tggttcgtat tcgacatgca gggaaatcgg cccggtgaag
     1681 cgcgcttcgg ccagcattgt gaagaacctc ccccaatcga ccatgccctc gcccaaagga
     1741 caccacttca cgcgccagcg cccgcccgat ttttcccaat aaaaatcctt gagcgcgacc
     1801 attttaaggc gcggcagcgc aatccgcgcc gacagaatcc agttgaagct tccaccttcg
     1861 atggtggcgt gcgcggggtc gaaataatac ccgatccaag ccggatccag gtccttgata
     1921 atatcgcgca ggtcccaaat cgccgtaccg acgtagttgc ccgaatggtt gtggaatccg
     1981 gacacgattc cgtattcctt ccccatttgc accagaccct cggtagcctt ctttacctgc
     2041 tggatggtat cgagcactgc gggagtcctg tacagccagt atccgggctt gtacaggggg
     2101 atcttcaacc gggacgccgt ggagagaatc gggcgtgtcg tgggctcatc cggccggagg
     2161 atgttggtgg tgatcatcgg caccgtgacg ccatgcgaac ggatcgcttc cacggcccgg
     2221 ggcatatcct cttctgcgcg ctccggcaga acgtgcgatt tcgcgcgcac ggtcaggtca
     2281 accccgtcga agcccatctc cctggaggtc tttccaagct gctcataatt caggttcgcc
     2341 atgtgcttgg aaaatatgca cagggagggt tttccactgc cttggctggc agccagggga
     2401 gcacccgccg ccagagcgac aaagtcacgc cgagtcaaat tcatatttat ttccctccac
     2461 aggcgcctgc cggcggccag tacgtcaaaa tactcttata tcctaaacgt agatgactcg
     2521 ccgaattgca ggactgctgc tcatgagtac ctggatgggc atggccgctg gcccaatcgc
     2581 cgtacacgga catcgcggcg cgcgcaccgt tctgcccgaa aacacgattc cggcatttga
     2641 atacgccatc gaacaccgcg ccgatttcat cgagctcgat ctcgccgtaa ccaaagacaa
     2701 cgtactcgtc gtctcgcatg accccatcgt gaacgagacc atctgcaagg ggccgcgcaa
     2761 gcaagccgtc atccgcgaaa tgacagcggc ggaactgcgc gaatgggatt gcggagcgat
     2821 tgccaacccg gaatttcccc ggcagaagac ggtgcccggc acccgcatcc caactttcga
     2881 tgaagtgctg gcgctcgccc cgcgtggcaa cttcggattt acgattgagc tcaagagcaa
     2941 cccccaaagg ccgcagttcg caccggctcc ggacgagttc gcgcgtctcg tcgtcgatgc
     3001 gatccgccgc cacaagctgg aggaccgctc catcgttcag tccttcgact tccgcacgct
     3061 gcgggcaacc agaaagattg cccccaatat ccggttggcc gccctctatt cgggtccgcc
     3121 gaaggacttt cccctgatcg cccaagaggc acaagccgat atcgtggcgc caaatttcaa
     3181 actcataacg ccggagcaga tacaggccgc ccaccaggcc ggaaagcagg tggtgccctg
     3241 gacgcccaac gaaccggcgg aatgggaccg cctgatcgcg gccggtgtgg atgcgattat
     3301 tacggacgat ccggcgggat tgatagcaca tctcgaccgc aaggggctgc gataatctat
     3361 tcggggattc gggacagcga gccgcggcgg attcaattcg cgctgaagta cacgttttga
     3421 ggcgctactt cctgatctgt tttaaggcgc gggtaataaa agcttcctgg tcgccctttt
     3481 tccaggagtc ttgaatctcc ttttcgggga aaccagcgac ccgccccagc aggatataag
     3541 caggtaacga gtgcggcaaa tgtttccagc gcgccattcc ggcagtggag tccagcccac
     3601 gttcccgaat cgaatcgagt acggaggggt cccggctttc ggtcaggttg acgagggcga
     3661 ccgcggcgct atgacggtcg ttccagagga tggagttcag catctcgaca aaccaggtgg
     3721 gcgagacctt tagccccagg tctggtttcc tctgcgacag caccatcatc gcgcccagcg
     3781 agcgcatggc gttgctgcgg acggtttcat cggggtcctg catcgctgtt tgcagatcgt
     3841 tcaccacggc ctgcttatcg ggcgcatagc ctatcagata agcggcgatc gcccggtgct
     3901 gttcgtcgcg cgagttcaac aaagtgtctc gaattgtgct caattgcgcc gccgcaatcg
     3961 taaggaactt ctcctgcacc cgccgtgccg ccccgttctg catcatggaa tggccggccg
     4021 tcagatcttc ccccgtttct ccctggcgca ctgcctcctg aacgcaatcg agaaagctct
     4081 ggtaggtgtc gagaatatcc gtagggagcg tcacatcccc ctcgggaaag cttctcagtg
     4141 caaaatgaga ggctcctttt tcttcgatac cgacgtaaag gattgccttg cccgcctcgc
     4201 agcataccgc ttccaggctg gccagcacca cgcctggaag cttttcaatc gcttcctcta
     4261 catcggattt cgaaggcggt agcggatcgc cctctttcac cgtcagaacc ttgcggacac
     4321 tttcttcgga aaccttcttc aggccgtaga agtcgatgat tccaattcgg ggggcctggc
     4381 ccgcgagcag gccaggcatt agtaacgcta tgaggcaggc agttcgcaaa tcaatacgac
     4441 gtatgacacc agcatccggc tacgcttgtt tttcagccaa cggacccaca acaggaagca
     4501 ccactctctc gttctgatac gccttccaca tcatatacag ccagaggatg aaaaacccca
     4561 gcccgaggac tagttgcagc atccctacca ggctgccaag ccagaacgaa atcgcgtaga
     4621 acatcatcgc cagaatgcca atggcgacgc tgatgacaaa aactccaata ctgacaaaga
     4681 tcgattggaa tgcatgaaac cgcacggtct tattgcggtt gtagggctcg atcaccagaa
     4741 agatgattcc tgtgatgaaa ccgagcaggt aacagagagc cgcggccaca tttgtatcta
     4801 aacccgccga agccgtggga ggtgctggtg atgcgggccc tggtggaaca ctatcagcct
     4861 gaaccgaagc accacatttc gcgcaaaagc ggccttccac cggagagccg caactaggac
     4921 acaaagccat ctcaacctcc gcaagagatc aatccgtcaa taacttatcg cgggaaacca
     4981 gagaaatgca agcactgctt tgaaactgca cgtacttcca gccgggatcg ccacctcttg
     5041 aggacggatt accggcattc cgtagtgtgt aatatgattt gtgtatccga acacgagata
     5101 aacatgaact actttgaccg cagacacttc ctttttgcct cggccgcggc ggctcaggca
     5161 gcgctggccc aacagaccgg agacagaatt ggtacggcga tgatcggcac gggcaatcgc
     5221 ggctcccatg tgctgcgcgg ggtactcgag cagccgaatg ctcgagttgt ggcgctatgc
     5281 gacatcaagc ccgaccggct cgacgcggcg gcgagctcgg cggcaaagga caaccccaaa
     5341 acgtacacgg actggcggcg ggtcatcgac aacaaggaag tggaggccgt tttcatcgca
     5401 acaccgccgc acctgcatgc ggaaatggcc atcgcagcgc tgaaggccgg caaacacgtc
     5461 tattgcgaga agccgatcgg tgttacagcc gaccaggtaa aacagcttgt acgggccgcc
     5521 aaaggcgtta acaaagtctt cgtctcgggc cagcaactcc gctcacacca gcagctcaag
     5581 gaagcagtca gcaaaattca ccagggcgaa ctcggcgagc ttttaatggt gaaagcgcag
     5641 cggcatgcta ccacggatct gaaccacgag ggctcatccg ccgactggta tttcgatgtg
     5701 acaaagtcag gcggctatct catcgagcag tccgtccaca atctggatgc atgtaactgg
     5761 gttattgggg ctcatccgac gcgtgctacc ggcttcggcg gcgtccagct ttacaagaac
     5821 gatcctccgg gccgcacaat catggacaat gcgagcctga cgttcgagta cccgagcgga
     5881 ctcaagctgt cttttaccca gaacgtattc catcccagcg gcatgccctg tgggggacag
     5941 tacatccatg ttttcggcag caaggcgtcg gtggatctgc tatatgccat gaacgaatat
     6001 cctctaccca agggcgaggt gaaagtaatt gccgaaaaac gccaggagcc ccagcacgca
     6061 cacacgaccg cattttacga ctgcattgtg aacggagcgg agaacccggc cgacatcacg
     6121 gtgggagcca ccgcggccct gacctccatc ctggggcatg aggcgatggt ccgggaacgt
     6181 gtcgtgaact ggagagatct cggagtagaa gtttaaaacg tggcgtcaga aatgattgcg
     6241 caacggctcc ctggcggtcg tggctcagca ttcgtttccg agtttttcat gaccctgcgg
     6301 gtcacaacca gggatgaaaa ggtctcgagc cggccggcca tcgccgtgat agatgcgata
     6361 gtgctgctcg ccgatgacag gtctcgtatc ctgcgcgctc agaaggagcg cgaaagcgag
     6421 aagacaagtg agggccggaa gcttgccttc caggtgcgcc acgctacggc tctccttcga
     6481 tgcgcggcgt atcgtgctcc tggtcgagca tggagccccc gatgtcttta gcctgcatcc
     6541 acagctcgaa gttagaggcg ctggtgttca gcagcgcgac gagttgcatg tacgcatcgg
     6601 tggtcagcgt gatgctgacc ggtccgacgc tcaggtgaag gttaccgcaa gctccgcaat
     6661 cgtagatccg tccgaatccg gggacctcgg cgagtatgca tccgttgttt tgcatatcta
     6721 ctccactgaa cgttgaatgg aaaccggctc gatcaagtcg aagtttttcc tgcgggcggc
     6781 accgccgatc accaggatgc cgtccttgta agagacaagc cggtgggcga ctctgcctgt
     6841 cgccttaccg gccttctccc atcgcgaacc atcgaggcga aacaacgttc catcggacac
     6901 gctcacgtac aggctgccgt tatgaaccgt ggcggctgga gcgaacgcca tcacgccttc
     6961 gggaagagca ggtccttcac tccacgtacc ggccttggga tcgtaaatcg aaaccttggg
     7021 ttcaatctgg tctttgtcgt tgatgccgcc gatcacgtaa agcttttcct gaaacgccac
     7081 ggcgatcagg gcgcgccggc ggaagggttg cgccgcggac ttccattcaa gctttggctc
     7141 cgaaagattc atcgtcacca gggtgtccgc ccacttctgc tttgaaccag ccagggtcca
     7201 tcctccggtg acgatcatct gatttccaac caccacaacg tcgtgcgagg atcgcgcctg
     7261 cggcagcggc ggaagagctt cccacttcct tgtcgccgga tcatagcggg cgcagccgga
     7321 agtagagcgg ttgtcggcgg gctcgcccgg tttgttacgc ggcgccatgc cgccgatgcg
     7381 atagattctg ccgttgtgcg ccgtgaggtt catcccttgc agcccgggtc cgggaggtaa
     7441 gtcttcccat tgcctgctaa cgaggttcaa ccgcgaaaag cggcccgaaa cggattcggt
     7501 ggaatagcta tgcgtggcgg cgatgtggcc gccgtaaacg tacagccacc catcgctttc
     7561 aacggcgccg aagctcgatg cggcttcagg cattgtggcg aacacagacg cagcggctgc
     7621 atcgaacaca agcgtcgcgt aatgccgcag ctcttcatat ttcttgccgt tgcgttctcc
     7681 gggaaccgat tcccagaatc gtgcccaagc gccaaagcgg ccagtctgcg aaaaggtctc
     7741 agttagccct gactcgtcgg tcttcactat cttctgagtc gcatcgggaa gtatgaccgt
     7801 gatctccgca tgaggaacag gtttgccgcg tccgacaagc ttcagtttga gatctcccgg
     7861 tttgccgatg ggaacgatct ctaccggcgt ctcgccgccc acgacggtct tcgggtcgaa
     7921 cgcatcgccc aggatggttt tcggatagta gagcagaaca tgtggtttcc cgctgccgcg
     7981 ctgcatgatc ccgagatcgg tgaacccgtg aacgacacga gttccggcac cggctagcga
     8041 cacctcgtac gcattcgcct ccttcaccag agtcagtggc gtatcgctgc cgtcgacagt
     8101 ccggacatat aacttcgtgc cgccgatgat tgcgacatcg actcccggct ccggcgtcaa
     8161 tgtctcgctc atgatcatct tggccttcgt gccgtcaggt tctgggacca caaaggtgaa
     8221 gtgcgcgtgc gccagagaga cacaagtcag aagcgggaaa aactggaaga accggctttt
     8281 catgaaacac ctcgaatctg agttgggatt gcttgcgcgc gtcgtcactc cacacctccg
     8341 aagcgccaac ggaggccgac gcggtattgc ctgccatagt cggggcgatc gaacgtgggc
     8401 gtagggagac tgagcttctg atcgcggcta ccggcgaaat tatcgatcga gaaataggct
     8461 tgagcgccgt ggacaaggtt ctttgaaacg tagaagtccc acacgctgta gccgaaggcg
     8521 cgtgttccgg cggcggggtt cagcagccac ttgctgaaga acattccccg gacattggcg
     8581 aacaaaccaa gccgtggatt gagatactcg gtccggacat agccctgatg tttgtgacgt
     8641 tgcggaagcc ctagttttgt atccgtgtcc ctggcgtcca ggtaagtata ggccgccgat
     8701 acctggaatt gccgcgtgat cgaatgcgcg gcgtcgagtt caaacccttg tgcgtagatg
     8761 cggccgaagt tctgatagat gaatgtctgg cgattcagca gcggcatgaa cgaagccgga
     8821 atgccatacg cgttcaaaag agcggtcaac tcttcgctgg tgcggggtgt tccgatattc
     8881 accgagtcaa tcaggtttcg gacgtcattg cggaacagac tgactccggc gcgaaaacgg
     8941 cgtgcgcgga aatcgacgcc agtcgaaaaa ctgcgagagg tttccggctc gaggttcggg
     9001 ttgccgatca cctgataaaa gctggccgga ttcgcgaatc ggaagtagag ctggccgagg
     9061 tcgggcgcgc gaaagcccct tccaaaggag ccgcgcgccg taaaatgatc gctgagccgc
     9121 acgaccaaac ccacctgggg cactgcccat gtcccgaaca acgagtggct ggtgacacgg
     9181 cctccggcgt ccagcgttgc acgccgggtt aggtggaact tgtcctggag ccagacatcg
     9241 ttcgaagtga cctggcgtcc gtcgttgtcg ccgaccagtc ggtttgcgcc tttgtagttg
     9301 tcctggaccc actcatagcc gccctggaga aggttgcgcg agcccaactg gtgcgagatc
     9361 gatccgtcga gccgtttgta ccgctggttc aggttggcaa gggccggcgg cgccggcgct
     9421 ccgagcgagt cgagccgtga attctcgtca tatcgcgccg aataggcccg cagttgcatg
     9481 gtcgttcgcg aggcaggcac gatatcgccg accaacgcat agttctgcac gctgtcgttg
     9541 tagagtccga gcaccggacc cgtttcgctc gcatttctgc cttcctcccg attgcgatag
     9601 gcgttcgcgg agaagccgag cgtgaagcgc ggcgtgaatg catagcgcgt cttgaagagc
     9661 agatcgtccc tgcgaaaatc gggaccgacg gtgaacaagc tattgggtag cagagaataa
     9721 gaatcctgac ggtggtgctc cagatcgaga aatatggaca ggtttttata gcgagtcccg
     9781 atatcgccgc ggccgtcgaa gatcccaagc gaaccgccgg aaaagttcag attgccctga
     9841 agcttctgcg tcggctcgcg tgagatcata ttgatgactc ctccgatagc gtcgctgccg
     9901 aacagggtgg atgccgctcc tttcgccacc tcgacgcgct ccaagcgccc ggtggactgg
     9961 cgattaagat tgaggttccc gctcttgatt ccccgtgcgc caatcatcgg gagcccgtct
    10021 tgaagaaccg cgacctggcg tgcgctgatt ccgcggatct gctccgtgct caccgaaccg
    10081 gacgatccac tgcgcgtcac cacgccgggt atttcgctca gcacgtcgga caagcgctcg
    10141 tatcccgttg tgatcatgga ctggcgcgtg acggcttcca ctttcacggg tgaatcctgt
    10201 tgcagttcct ccacacggga acccgatgtg acggtgatct gttcccggat tccgccaacc
    10261 gaaaggcgga cgtccatcga tacggtttcg tttcgccaat caacgtaccg ggattcgtca
    10321 gcgaatccct gggctcgtat ctgcaccaga taccggcccg acagcggcgg cctcaactcg
    10381 acggcgccgt cctggccagt tgcctggtcg aaagagaaag cgcccaatgt ggagcgcacc
    10441 gttatgcgag cacccaccag gggcagtgcc gccgcgtcag tgacggaaat ccgcagaaca
    10501 gggggattgg ttggcgcctg cgataatgcg gcgccggcga tggtcagcgc cgcgagagcg
    10561 gcggaaagct tacgatagcc agacatggca gtttcttccc ttgtgaaccg cgggcgggcc
    10621 acctagaata gagatgtcgc cagcctacgg gttgtcgatc caccaggctc gggcgctgct
    10681 cggccaagag ttacgcgcct acgcctggat aaattgtgtt atcttccggt ctcgtgttct
    10741 tgaatcccag cttgcgtcag tctattctaa atgcaactgg acgtcaagta agaaacgctg
    10801 atgccactca aaatcagttg tgttctgtga tttttcaaca gcaaagcccc attctcagtg
    10861 gacatatttg taagtcagtt gcagtttgat tgccggtctt tgccatgcag cgtgacgccg
    10921 aggggcgcgc aggccgtttc tcaatttaat tagggagtgg ttatcggcac gcagtccgcc
    10981 gatgaaacta atgacgattc tggtccgcct gtagaacccg gtgattcagg taggaaaccc
    11041 gaaccggaac tccggcgtta aaactgacct tcaccaccac ctgatagtgc tccggaagct
    11101 gtccggaggg gaggcgaatt ctgctaacca gatcgcgggc gtattgcggc tgtgtcatat
    11161 actgcgtggc gcccatggtc cccacggtcc agttcccgga cagaatcaac atctgtgcgt
    11221 tgcggtgagg cccgggcaac cggctgatca agccgtgggt ttccccggtg tcccgggtcg
    11281 acgatccttt ctcgatttcg tccggaaaat atgccggttc gccggcctcc ggatgcaaat
    11341 tccgtattcc gttatcctcg aagacaaact cctgactgat cgggattcgc cggagctgcg
    11401 gattgtactt cggcggaccc aggaaaatca cgttgtgggc cacgatctgg tcccaggaaa
    11461 gcctgctgct ggccgtcacc agcacgtctt ccctaacggt cccgagcagc ttgcccagca
    11521 gaaacgcagc acgggcttct ccgacccccg tgaagctgtg ccagggaatc gggtcggttt
    11581 cgcggagcga ttcaagcagt ttgttgatcg acggagacgt gcgcgcctgc tcccagtcgt
    11641 aagcggcggg aagacggacg aacgcgtggg catccgggaa gtggatgaaa aagggctcgc
    11701 caatacagac cgtggtgggg cgccctcccg cgaggaacgg actccac
//
LOCUS       DASTMK010000350        14794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_433435, whole genome shotgun sequence.
ACCESSION   DASTMK010000350 DASTMK010000000
VERSION     DASTMK010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14794
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433435"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFQ79_15375"
     CDS             <1..366
                     /locus_tag="VFQ79_15375"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU0141103.1"
                     /translation="EFDREFLRYQLEHHKEDIREFEKTADKAENPEVKEFASNALPLL
                     KKHQDMVAQAAQAAGISIEREGTADRMRDEQQRQSDPTLDYPQRHGDPSQDPRQRPMD
                     PAQQPQQRQYPETTPPIVP"
     gene            432..1088
                     /locus_tag="VFQ79_15380"
     CDS             432..1088
                     /locus_tag="VFQ79_15380"
                     /inference="COORDINATES: protein motif:HMM:NF022620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 family protein"
                     /protein_id="HEU0141104.1"
                     /translation="MARYCFAAFLVLSILPGCSRLYYASMEKIGKEKRDILVKRITQG
                     KQDQEEARQQIQTTLEAFKELTGFEGGELERVHDKLNREYERSRDRANDLANQTKSID
                     RVARDMFAEWDKEIGSMRNSSLKQKSRSLLRDARARHEQYMAAMRSTQRKMEPVLQAF
                     YDQVTFLKHNLNARAIQSLKKTVAAMDTQVEALVADIERSIKESDAFIKTLTEPDHTS
                     "
     gene            1161..2540
                     /locus_tag="VFQ79_15385"
     CDS             1161..2540
                     /locus_tag="VFQ79_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0141105.1"
                     /translation="MLRLDADRAVEFCDGLRRRDFLHAGSLSFLGLGLTQMFGLKALG
                     AVDNRKKDINCIFLMLVGGPSQLDTWDMKPDAPVEIRGPYKPIKTNVPGIEVSENFPR
                     MAKNADKFALVRSVYHTAAAVHDTGHQMMQTGRLFQGGVEHPHIGCVLGKLKGPNGDV
                     PAHVLLPRSIGNTGGNMPHGQNAGYLGKTYDPFVLNADPSDPNFRVPDMLPPEYLSAL
                     RVDRRKNWRELVDRTVSKFETSQDARLFDATFHQAYTLMSSAKAREAFELHREPEAVR
                     QKYGMNRFGQSCLLARRMIEAGVRFVTVNMFETVFDEITWDIHGSKPFSPISCYSDLV
                     GPMFDNAYSSLLEELHERGLLENTMVVATGEFGRTPKVNPAGGRDHWPQCWTMLLGGG
                     GIKGGQVIGSSDEIGALPKDRPTSPAEIAATIYYGLGIDLELELPGAQGRPLPLVDRG
                     VEPVRELFS"
     gene            2565..5009
                     /locus_tag="VFQ79_15390"
     CDS             2565..5009
                     /locus_tag="VFQ79_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEU0141106.1"
                     /translation="MKQVLPFLFSGMLWAAPAPSSLSLLPASVNLSGPEARQQLLAEA
                     TVDGYNEDWTTIAVWKSSNPRVAAVDGAGIVRPVADGEATITAAAKGMTASAKVTVKG
                     AAAPFTWSFRNHVIPVFTKAGCNQGACHGALAGKNGFKLTLRGYDPEVDYNTLTRESV
                     GRRISLAEPGRSLMLLKPTFALPHGGGKRFGIDSLEYRILSEWIAAGTPPPAPKDPEV
                     MSLEVFPKNAKLKPDAQQQLVVLARYSDNHVEDVTRWVKYSSNNEGVATVDDSGRVKM
                     TGRGEAAVTLWYLSRVLYSRVVTPYDHQVPPEVYTQAPVENYIDGFVNAKLKSLNLAP
                     SKQASDSTFIRRAFLDAAGILPNSEEVETFLKDSSPDKHRRLIDNLVEREEFVDYWAY
                     KWSDLFLVSSRKLGSGSMWAFYKWIRESVKNNTPWDQMAREIFTGSGSNRRNGALNYF
                     VLHKDPIDLAENTTQAFLGQRLTCARCHNHPLEKWTQKQYYQFANLFSRVGIKNGGEP
                     GEVVVYGKASGDINHPRLLRPLAPAPLDGEPVALDSAEDRRVAFAEWLTSPENPYFAR
                     SIVNRVWGALMGRGLVDPVDDVRATNPASNEELFTALTKDFVTSGFDVKKLIRTIMSS
                     AAYQRSSDSNETNQHDNIYYSKYILKRLPAEVIADAMSQVTGVPANYAGYPAGTRAMQ
                     LPDTRIASRFLTVFGRPERVICDASERSSDPSIAQALHVINGDSLNRKLSAPDGNISL
                     FLKLGLSDARILEYLYLSAFSRYPTEAELETLGSALAKARLTTGTPEAIREARRQALE
                     DMTWALLTSKEFVFNQ"
     gene            5006..6325
                     /locus_tag="VFQ79_15395"
     CDS             5006..6325
                     /locus_tag="VFQ79_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEU0141107.1"
                     /translation="MRRRWYILLTPVIAASVGARAEEPSYSRDVAPVFEKYCVSCHAA
                     QVKLGGLELDTFEGATKTGAHGPVIVPGKAEESRLFLMIAGKVKPVMPMDGKILASGE
                     IDSIRRWIDAGAKFDGSAPATAAPKTAIPDIRPRKAVKPQVFAIAYSPDGKTLALAGY
                     QEVRLLDAATRKTVATLGGHSGSVRALAFSSDGKLLAAAGGLPGQKGEIRIWDAATHI
                     LVRSITGHNDCIYAVAFPPDAKSIASSSYDKLIKIWSVETSEEIRTLKDHIDAVYALA
                     YTPDGKRIVSGAADRTVKIWDAVSGRRLYTLGEPQDGINSIAIHPSGKYVAAGGLDKS
                     IRIWSLGETSGTLVQNSIAHADAILKLAWSPDGNLLVSSAADRTIKAFRPDLTEVQTI
                     GAQPDWAYAVEFSPDGKVFAVARFDGTLSIYDTEQFQDIIATQLAGR"
     gene            6363..8783
                     /locus_tag="VFQ79_15400"
     CDS             6363..8783
                     /locus_tag="VFQ79_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEU0141108.1"
                     /translation="MKWAWLLMLIPAMTAGAVEEHPTGSRTTPPIIQSVSPRGLPRGM
                     TAEFTVEGLNLADASAIYFSEQRLSGRILRIKELPDLPEVRLGSAGTPSTVDLGPLPP
                     RNQVTIEMDIAPDATVGPVSFRLQTPLGTTSESRFVVEPFYGEAPDREPNDTPEQAFE
                     TYLPAILVGAISKPGDVDFYKITAQAGEQIVFDNAGAELGSSLEPVVTILTPDQATLK
                     EFGPEGVEDANRFAYTFESAGTYYVRIADYEQRGRGSNFYRIKAGAFPIVTSAYPLGL
                     QRGSKKNVHLTGYRIGPGTMVVEGQPSAGEEDSITLRPSSPAGPAFSTVRLAVGVDPE
                     VEASGTNTTATAAQRIAAPLTVNGRIPAPQNGAPGEHYYRLAARKGEKLVIETLARRY
                     GSDLDSLLEILDAKGQPVERATVRAVWETTTTLAERDSVQRGIRMQAWNSIAPGDYLM
                     IGGEIVRVESLPKSPDADVVLEGFGGQRLTYFDTSAEAHAVDKPVYKVQIYEAGKQFA
                     PNGLPLARLYYRNDDGGPGYGKDSIVRFTAPADGEYLVRVRDIRGEGGDKFAYRLNVR
                     PPRPDFRLRATPENPNIPRDGYVPLTVTAFRMDGFDGPVEVSVQELPEGVSATKAVIA
                     SGQDSATLLLSATGQAKLDRALPLKITGEAKIEGRPVIHAANAGEKLQLVSLMPQPDV
                     VMTAETQEVVVEAGGTAEIAVSIRRQNDFGGRVPVDVRNLPPRVSVLDVGLNGVLINE
                     EEDRRSFTIEALPNAEPVEQLIYVAARVETRSPLPSSYAAPQAIRLKVLPRKEVSQAK
                     TASAAGAR"
     gene            complement(8780..9652)
                     /locus_tag="VFQ79_15405"
     CDS             complement(8780..9652)
                     /locus_tag="VFQ79_15405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0141109.1"
                     /translation="MSYFHRRTFLHSAVVALTAFVKGNPLSAEEVTETRRGIFDAHLH
                     IPADNGEVFQWNLVTRTMPEFVAYLDKCGVRRGVISSARSNTAKTPEDYRAGNREVAK
                     YAEKYKGRFRGSCVITPFRIDEALREIEECHKQLGFVWLGEFCNYMTGYRYDTPEWTQ
                     IMNLAKRLNVVTQIHTNAREMRYLADTFPENTIVFPHLGGNQKDIQERVDIVAQHKNA
                     YIDISGSGIERVGILEKAVKTIGADRVLYGSDFTINEPSGVIARVNNAFLTAEDREKI
                     LFRNLERLLNRRSG"
     gene            complement(9701..10363)
                     /locus_tag="VFQ79_15410"
     CDS             complement(9701..10363)
                     /locus_tag="VFQ79_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU0141110.1"
                     /translation="MVLAGIGLILTIGVFLLSAGARGPFPLTGRSHELPNPETGSSRR
                     AALIGMFLALLSILMFFAAFSVAMFARRGTSDDWVSLPLPDILWINTGLLVASSVCLE
                     WARRSLHSGRREVFNFCWTMGVLLGIGFFFGQCTAWSELLENGFYVNSGPSSSFFYMI
                     TVAHAIHLTGGLFCLLYVEVEALRFRLGPAKRTFVDVSRNYWHFMGAIWVYVILLFQF
                     WG"
     gene            complement(10456..11118)
                     /gene="fsa"
                     /locus_tag="VFQ79_15415"
     CDS             complement(10456..11118)
                     /gene="fsa"
                     /locus_tag="VFQ79_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU0141111.1"
                     /translation="MKFFLDTANLGELKKAAAWGIVDGVTTNPSLIAKEGRPIAEHIK
                     AICDIVDGDISAEVVSTEAGEMLKEGRELSRIHKNVVVKLPLTRDGITACSKLSKEGI
                     RINVTLCFSPAQALLAAKAGAYIISPFVGRLDDIGAIGMDLVRSITSIYKTHGLATQV
                     LAASLRSPLHVVDAASAGAHIATMPFKVLDMLFNHPLTDKGLEQFLKDWNKVFEETPA
                     AR"
     gene            complement(11123..11914)
                     /locus_tag="VFQ79_15420"
     CDS             complement(11123..11914)
                     /locus_tag="VFQ79_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0141112.1"
                     /translation="MPKRIRFSLNPNIEPTVPDSSNEVKYGIVENGTIFETPSVDSVE
                     KGASHAMSSVRLLPPCWPSKIVCVGRNYPEHARELGNEPPAEPMIFLKPPSSLIADGD
                     RIVYPHISGRVDYEGELGVVISRRARHVRREEAFGYISGYTCVNDITARDLQKKDGQW
                     TRGKGFDTFCAVGPWVVPASEARLDELRVQTYVDGVKKQDAPISEMMFPVDAIVAFVT
                     EFMTLEPGDLIATGTPPGVGAMQPGSVVRVEIPGLGAIENLVVRG"
     gene            complement(11918..12910)
                     /locus_tag="VFQ79_15425"
     CDS             complement(11918..12910)
                     /locus_tag="VFQ79_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141113.1"
                     /translation="MRRLLAAALPLVLAGCGSIGDPLPPALNIPERILDLRVKQSGGM
                     LVLDFTRPQRTTEGLVIDKPGTIEVAAGPAGEGEFDHERWAAEATRFEFPSEPTESSQ
                     PIEVPAQEFTGKEILLGVRALNKRGRTAGWSNLVTLRVIAPLPVPSKVQAESAAEGAK
                     VTWAYPERAAGLKFRVYRRAPASSEMAQAGEVETLEWVDAAAEYGSRYEYQVQAVRED
                     GDIRAESKHSPPVAIVPVDTFPPAAPSGVIGIPGAGTVELTWERVANRDLQGYYVYRA
                     AGDGDLAKISGLVEAPAYSDATVESGKAYQYAVTSVDRLGNESQRSTPVPITAP"
     gene            complement(12915..14273)
                     /gene="hslU"
                     /locus_tag="VFQ79_15430"
     CDS             complement(12915..14273)
                     /gene="hslU"
                     /locus_tag="VFQ79_15430"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU0141114.1"
                     /translation="MVIYLPTQSVDEAPLLDELTPREIVKELDKYVVGQPEAKRAVAV
                     ALRNRIRRQKLEPEMAEEVMPKNILMIGPTGVGKTEIARRLAKLANSPFLKVEASKFT
                     EVGYVGRDVESMIRDLVDIAIDMVREERLDEVADRAERNAEDRVLDLLMPPQGESSDS
                     IERTREKLREKLRTGKLDERTVELDVKERGPTFEISTNAGIEEMDVNLKDVLPGLFGG
                     RTRKRKMRVSEALEYLVQEEEQKLIDMEQVTRIAIERVEKNGIIFLDEVDKIAGRESG
                     HGPDVSREGVQRDILPIVEGTTVNTRYGFVRTDHILFMAAGAFHVSKPSDLIPELQGR
                     FPIRVELRSLSEQDFIRILREPKNALIKQYMALLETEGIKLSFTPDALEEIARSAARV
                     NEMAENIGARRLHTIMEKLLDDISFEGPDLKRKTVKIDRAYVNKQLAEIIKDQDLSKY
                     IL"
     gene            complement(14273..>14794)
                     /gene="hslV"
                     /locus_tag="VFQ79_15435"
     CDS             complement(14273..>14794)
                     /gene="hslV"
                     /locus_tag="VFQ79_15435"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEU0141115.1"
                     /translation="STTILCVRRGGKVVLAGDGQVTFGQEVFKASAKKLRRLYNDKII
                     AGFAGSTADAFALFARFEAKLEQYSGNLPRSVVELAKDWRTDKMLRHLEALLLVADLK
                     STYIVSGGGDVIEPDEDVAAIGSGGPFAKAAAAALIENTKLPAREIAEKSMAIAGKIC
                     IYTNENIVYEELG"
BASE COUNT         3072 a         4388 c         4390 g         2944 t
ORIGIN      
        1 gagtttgacc gggagttctt gcgctatcag ctcgaacatc acaaagagga tatccgggag
       61 ttcgaaaaaa cagcagataa agccgagaat ccggaagtga aagagtttgc gtccaatgct
      121 cttccgctgt tgaagaaaca tcaggatatg gttgcgcagg cagcccaagc cgccggcatt
      181 tcgatcgagc gggaaggaac tgcggaccgg atgcgcgatg agcagcagag acagagtgac
      241 ccaacactcg attatccgca acgccacggt gatccgtcac aggatccgcg tcagcgtccg
      301 atggatcccg cacagcaacc gcagcagcgg cagtacccgg aaactacacc ccctatagtt
      361 ccttaacatg aggagtggcg gcgtccgatg ccgtcactca cctttcccaa ttgtgtctta
      421 gactgattac agtggcacga tattgcttcg ccgcctttct ggttctatcg attctccctg
      481 ggtgcagccg gctgtactac gcctccatgg agaagatcgg caaagagaag cgcgacatcc
      541 tggtgaagcg aatcacgcaa ggcaagcagg atcaggaaga agcgcggcag cagatccaaa
      601 ccacgctgga agcattcaag gaactgaccg gcttcgaagg gggcgaactc gagcgcgttc
      661 acgacaaact caaccgcgaa tatgagagat cccgggaccg cgccaacgat cttgccaatc
      721 agaccaaatc catcgatcgt gtcgccaggg acatgttcgc ggaatgggat aaggaaatcg
      781 gcagtatgcg gaacagctcc ctcaagcaaa agagccgcag cctgcttcga gacgcgcggg
      841 cacgtcacga gcagtatatg gcggcgatgc gttccaccca acgaaagatg gagccggttt
      901 tgcaggcttt ctacgaccag gtcacgtttc tcaaacacaa cctgaatgca cgagccatcc
      961 agtcgctgaa gaaaacggtt gccgcgatgg atacccaggt ggaagcgctg gtagccgata
     1021 tcgagcggtc cattaaagag tccgacgcct tcatcaagac actcacggag ccggaccaca
     1081 catcctaaag ggcggttgcc gccgctgatc aatcgggtat aaactgtaaa ccactattcc
     1141 ctgggaggag ttttcctgcc atgttgcgtc tggatgcaga tcgggccgtg gagttttgtg
     1201 acggtcttcg ccggcgggat ttcctgcatg ccggctccct ctcttttctt ggcctcggcc
     1261 tcacccaaat gttcgggctg aaagcactgg gggcggtaga taaccggaag aaggatatta
     1321 actgcatttt tttgatgctc gtcggcggac cttcgcagtt ggacacatgg gacatgaagc
     1381 ccgatgcgcc cgtggagatc cggggtccgt acaaaccgat caagacaaac gtacccggca
     1441 ttgaagtatc ggagaacttc ccgcgaatgg cgaagaacgc ggataagttc gccctggtac
     1501 gtagcgtgta tcacaccgcc gcggccgtgc atgacaccgg ccatcagatg atgcagacgg
     1561 gcaggctgtt ccagggtggc gtcgagcatc cgcacatcgg gtgcgttctc ggcaagttga
     1621 aagggccgaa cggcgacgtg ccggcacacg tgctgttgcc gcgcagcatc gggaataccg
     1681 gcggcaacat gccgcatggt cagaacgccg gctatctcgg aaaaacttac gatcccttcg
     1741 tgttgaacgc cgatccgtcc gatcccaatt tccgcgtccc tgacatgctt ccgccggaat
     1801 acctgtccgc gttgcgggtg gaccggcgca aaaactggcg ggagctggtg gaccggacgg
     1861 tcagcaaatt cgaaacctcg caggatgcgc ggctgttcga cgcgacgttt catcaggcat
     1921 acacgctcat gtcatcggcc aaagcgcgcg aggctttcga attgcaccgg gagcccgagg
     1981 ccgtgcgcca gaagtatggc atgaaccgct tcggacagag ttgtttgctg gcgcgccgca
     2041 tgattgaagc gggagtgcgc ttcgtcacgg tgaatatgtt cgagacggtg ttcgacgaga
     2101 tcacctggga catccacgga tcgaagccgt tcagtcccat cagttgttac agcgacctgg
     2161 tgggacccat gttcgacaac gcgtatagct cgcttctcga agaactccac gagcgcggac
     2221 ttctcgaaaa tacgatggtg gtcgctaccg gcgaattcgg ccgcacgccg aaggtgaacc
     2281 cggcgggcgg gcgcgaccac tggccgcagt gctggacaat gctgctgggc ggcggcggga
     2341 tcaaaggcgg tcaagtgatc ggctcctccg atgagatcgg ggcgctgcca aaagaccggc
     2401 caacatcgcc ggccgagatc gcggcgacca tttattacgg ccttggcatc gacctcgaac
     2461 tggaattgcc gggtgcgcag gggcgcccgc ttcctttggt ggaccgcggc gtcgagccgg
     2521 ttcgcgaact cttctcctaa agttgttgtt tagctgggac tcgcatgaaa caagtgttgc
     2581 cgtttctgtt ttcggggatg ctctgggcgg cgccggcccc ttcaagcctg agcctgctgc
     2641 ccgcctcggt gaatctttcc ggtccggaag cacggcagca gttgctcgcg gaagccacag
     2701 tcgacggcta taacgaggac tggacaacca tagccgtctg gaagtcgtcc aacccgcgag
     2761 tcgccgccgt ggatggcgcg ggaatcgtgc gtcccgtggc cgatggcgaa gcaacgatca
     2821 cagcagcggc caaggggatg acggcttccg cgaaggtgac ggtaaagggt gccgccgccc
     2881 cgttcacgtg gagtttccgc aaccacgtga tccccgtttt tacaaaagcc ggatgcaacc
     2941 agggtgcatg ccacggcgcg ctggcgggaa agaacggttt caagctgacc ctccgggggt
     3001 acgatcccga agtcgactac aacacgctga cgcgggagtc cgtcggccgc cgcatctcgc
     3061 tggccgagcc gggacgcagc ctcatgctgc tgaagcccac tttcgcccta ccgcacgggg
     3121 gaggcaagcg cttcggtatc gattcgctcg aataccggat tctttcggag tggattgcag
     3181 ccggtacgcc gccgccggcc ccgaaagatc ccgaggtgat gagcctcgag gtatttccaa
     3241 agaacgccaa gttgaagccg gacgcacagc agcaactggt ggtgctggca cgctacagcg
     3301 acaatcacgt cgaggatgtc acacgctggg tgaaatacag ttccaataac gaaggagtgg
     3361 ccaccgtcga cgattcaggc cgcgtgaaga tgaccggccg cggcgaggcg gcggtgacac
     3421 tatggtattt gagccgggtg ttgtactcgc gtgtggtgac gccgtacgat catcaggttc
     3481 cgcccgaagt ttacacacag gcaccagtcg aaaactatat cgacggcttc gtaaacgcga
     3541 agctgaaatc gctgaacctg gccccttcca aacaggcttc ggattccacg ttcatccggc
     3601 gggccttcct ggatgccgcc gggatcctgc cgaattccga agaggtggaa accttcctga
     3661 aggattcctc accggacaag caccggcgct tgatcgacaa cttagtggaa cgggaagagt
     3721 tcgtcgatta ctgggcttat aagtggtccg atctgttcct ggtatccagc cgtaagttgg
     3781 gctcgggctc catgtgggca ttttataagt ggatccggga gagtgtaaaa aacaatacgc
     3841 cctgggacca gatggcgcgg gagatcttca ccggttccgg cagcaaccgg cgcaacggcg
     3901 ccttgaacta ttttgttctc cacaaagatc cgatcgatct ggcggagaat acgacccagg
     3961 cgttcctggg ccagcggctg acttgcgcgc ggtgccacaa tcatccactc gaaaagtgga
     4021 cccagaaaca gtactatcaa ttcgcgaacc tgttctcgcg tgtcggaatc aaaaacggcg
     4081 gcgaaccggg agaggtggtg gtgtatggga aagcatcggg cgacatcaat catccccgcc
     4141 tgctgcgccc gctggcgccg gctcctctcg acggcgagcc ggtcgctctg gattcagccg
     4201 aagatcgacg tgttgctttt gcggaatggc tgaccagtcc ggaaaacccc tactttgccc
     4261 gctccatcgt caacagggtc tggggcgctt tgatgggaag ggggctggtc gaccccgtgg
     4321 acgacgtacg cgcgacgaac ccagcatcca acgaagagtt attcacggca cttactaagg
     4381 attttgtaac aagcggcttt gatgttaaaa agctgatccg gacgattatg agctctgccg
     4441 cctatcaacg ctcgtccgac tcgaatgaaa cgaatcagca cgacaacatc tattactcca
     4501 aatacattct caaacggctt cccgcggaag tgattgccga tgccatgtca caggtaaccg
     4561 gagttccggc caactatgcc ggctatcccg cgggcactcg cgctatgcaa ttaccggaca
     4621 cgcgcattgc ctcgcgcttt cttacggtct tcggccgtcc ggagcgtgtg atctgcgatg
     4681 cgtcggagcg ttcatccgac ccgagcattg cgcaggcttt gcacgtgatc aacggcgatt
     4741 ccctgaacag aaaactgagt gccccggacg gcaacatctc attgttcctc aagctgggcc
     4801 tgtcggacgc gcgcattctc gaatatcttt atctctccgc gttttcgcgc tatccgacgg
     4861 aagcggagtt agagacgctg ggcagcgcgc tcgccaaagc ccgcctgacc actggaactc
     4921 cggaggcgat tcgggaagcg cggcggcagg cgctggagga tatgacctgg gcgctgctaa
     4981 ccagcaagga gtttgtcttc aaccaatgag gcgccgctgg tatattcttt tgacgccggt
     5041 gattgccgcc agcgtgggcg cgcgcgccga agaaccctcc tactcgcgcg acgtggcccc
     5101 cgtgttcgag aagtattgcg tgagttgtca cgcggcacag gtgaaactcg gcgggttgga
     5161 gttggacacg tttgaaggtg caacgaaaac gggcgcgcac ggccctgtga tcgtgcccgg
     5221 caaggcggag gaaagccggc tatttctgat gatcgccggc aaggtgaagc ccgtgatgcc
     5281 gatggatggg aagatactgg cgtccggcga gatcgactcg atccgccgtt ggatcgacgc
     5341 aggcgcgaaa ttcgacggct ccgccccggc gaccgccgct ccaaaaacag ccatccccga
     5401 catccgcccg cgaaaggccg tcaaaccgca ggtatttgcc atcgcataca gccccgacgg
     5461 aaaaacgctg gcgctggccg gataccagga ggtccgtctg ctcgacgccg ctacgcgtaa
     5521 aaccgtcgca actctgggcg gccattcggg ctcggtgcgc gcgctcgcat tctcaagtga
     5581 cgggaagctt ctggcggcag cgggcgggct gcccgggcaa aaaggcgaga tccggatctg
     5641 ggacgctgcc acgcatattt tggtccggtc catcacgggc cacaacgact gcatctacgc
     5701 ggtggccttt ccacccgatg ccaaatccat tgccagttcc agctacgaca agctcatcaa
     5761 gatctggagt gtagaaacaa gcgaagagat ccgcacgttg aaagaccaca tcgatgcggt
     5821 ttatgcactc gcctatacgc cggacggcaa aagaatcgtc tcgggcgccg cggaccgaac
     5881 ggtgaaaatc tgggatgcag tatccggccg gcgcctgtac acgctcggtg agccgcagga
     5941 cggtatcaac tcaatcgcaa ttcacccgtc cggaaaatat gtcgcggccg gaggcctgga
     6001 taagagtatc cggatatggt cgctgggaga gacaagcggg acgctggtac agaactccat
     6061 cgcgcatgcg gacgccattt tgaaactggc atggtcaccc gatggcaatc tcctggtatc
     6121 ttccgccgcc gacagaacaa ttaaagcgtt ccggccagac ttgaccgagg tacaaacgat
     6181 cggggctcag ccggattggg catacgcggt cgagttttcg ccagatggga aagtatttgc
     6241 ggtagcgcgg ttcgacggga cgcttagtat ttacgatacc gagcaatttc aagatattat
     6301 cgccacgcaa ctggccggac gctgaacgca atatctagag cgcgaaatct gggaggcgct
     6361 tcatgaaatg ggcatggctg ttgatgctta tcccggcgat gacagccggg gcggtcgagg
     6421 aacatccgac gggttcgcga acgacgccgc caatcattca gtccgtctca ccccggggct
     6481 tgccgcgagg catgactgcc gagttcacgg tggaaggact gaatcttgcg gatgccagcg
     6541 cgatctattt cagtgagcag aggctgtcgg gccgcattct gcgaataaaa gaacttcccg
     6601 acttgcccga ggtacggctc ggctcggcag gcacgccatc tactgtcgac cttggaccgc
     6661 taccgccccg caatcaggta acgatcgaaa tggacatcgc gcccgatgcc acggtcggcc
     6721 ctgtttcttt ccgtctgcaa acgccgctgg gaacaacttc ggagtcccgg tttgtggtgg
     6781 aacctttcta tggggaggcg ccggaccgcg agcccaacga cacaccggag caggccttcg
     6841 aaacttatct gcccgcgatt ctggtcgggg ccatttcgaa gccgggcgac gtcgatttct
     6901 acaagatcac cgctcaagca ggcgagcaga tcgtatttga caacgcgggc gcggaactcg
     6961 gctcatctct tgaacccgta gtaacgatcc tcactccgga ccaggctacc ctcaaggaat
     7021 tcgggccgga aggcgtagag gacgcaaatc gattcgctta caccttcgag tcggccggaa
     7081 cctactatgt ccgcatcgcc gactatgaac agcgtgggcg cggaagtaat ttctatcgca
     7141 tcaaagctgg agcgtttcct attgtgacca gcgcgtaccc gctcgggttg cagcgtggca
     7201 gcaagaaaaa tgtgcatttg accgggtacc gcatcgggcc gggaacgatg gtagtcgagg
     7261 gccagccttc cgccggtgag gaagactcta tcactttgcg gccttcctca cccgcaggac
     7321 cggctttttc cacggtgcgc ctggccgtcg gtgtcgatcc cgaagtggaa gcgagcggca
     7381 ccaatacaac cgctacagcc gcgcagcgca ttgcggctcc gttaaccgtc aacggcagga
     7441 tcccagcccc tcaaaacggt gcaccgggcg agcactacta tcgcctggcg gcccgcaagg
     7501 gcgagaagct ggtaatcgaa acgctagccc ggcgatatgg ctcggacctg gattcgctgc
     7561 tcgaaattct cgacgcgaaa ggccagccgg tggagcgtgc gacggtgcgt gctgtttggg
     7621 aaaccaccac cactctggcg gagcgggatt ccgtgcagcg cggcatacgc atgcaggcgt
     7681 ggaacagcat cgccccgggc gactacctga tgattggcgg tgaaatcgtg agagtggaat
     7741 cgctgccaaa gtcacctgat gcagacgtcg tcctggaagg attcggcggg cagcgcctta
     7801 cctacttcga cacttcggca gaagcgcacg ccgtcgataa acccgtgtac aaagttcaga
     7861 tttacgaggc gggcaagcag ttcgcgccga atgggctacc cctggcgcgc ctctattacc
     7921 ggaatgacga cggcggcccg ggatacggaa aagactccat cgtgcgcttt acggcaccgg
     7981 cggatggaga gtacctggtg cgcgtgcgcg acatccgcgg cgagggcgga gacaagtttg
     8041 cctatcgtct gaacgtccgg ccgccccggc ccgacttccg gctgcgcgct acgccagaga
     8101 atccgaacat cccacgcgat ggctacgtcc cgctgacggt aaccgcattt cgtatggatg
     8161 gttttgacgg accagtggaa gtttctgttc aggagctgcc ggaaggcgtg agtgcgacaa
     8221 aagccgtcat tgcttcggga caggattcgg caacgctgct gttgagcgct accgggcagg
     8281 cgaaactcga tcgggcactg cctttaaaga tcaccggtga agcaaagatc gagggccggc
     8341 cggttattca cgctgccaac gccggcgaga aattgcaact cgtttctctg atgccccaac
     8401 cggacgtcgt gatgacggca gaaacgcagg aagttgtcgt tgaggcaggc ggaaccgcgg
     8461 agattgccgt cagcatccgc aggcagaatg atttcggcgg gcgtgtgccg gtggatgtgc
     8521 gaaacctgcc tccgcgcgtg agcgttctcg atgtcggcct gaacggggta ttgatcaacg
     8581 aggaggaaga ccggcggagc tttacgattg aagcgctgcc gaatgccgag ccggtggagc
     8641 agctcatcta tgtagcggcc cgcgtggaaa cacggtcgcc gttaccgtct tcctatgccg
     8701 ctccgcaggc aatccggctg aaagtgctgc cacgaaaaga ggtctcacag gcgaagacgg
     8761 cgagtgcggc gggcgcccgt tagccggacc tgcgattcaa cagccgttcg agattgcgga
     8821 acaggatctt ctcccgatcc tcggccgtga ggaacgcgtt gttcacgcgc gcgatcactc
     8881 ccgacggctc gttgattgtg aaatccgaac cgtacagcac ccggtcggct ccgatggtct
     8941 tcaccgcctt ctccaggatg ccgacacgct caatccccga ccccgaaatg tcgatgtagg
     9001 cgttcttgtg ctgcgcgact atgtcaacgc gctcctgtat gtccttttgg ttgccgccga
     9061 ggtgcgggaa tacgatcgtg ttctccggga acgtgtccgc gaggtatcgc atctccctcg
     9121 cgttcgtgtg gatttgcgtc accacattca ggcgtttggc cagattcatg atctgggtcc
     9181 attcgggcgt atcgtaacgg tagcccgtca tgtaattgca gaactctccc agccagacga
     9241 agcccaattg tttgtgacat tcctcgattt cgcgaagcgc ctcatcgatc cggaaaggag
     9301 tgatgacgca gcttccacgg aagcggcctt tgtatttttc cgcgtactta gctacttccc
     9361 ggttgcccgc ccggtagtcc tctggcgttt tcgccgtgtt gcttcgcgcc gacgagatta
     9421 cgccgcggcg tacaccgcat ttatcgagat aagcgacgaa ctccggcatg gtgcgcgtta
     9481 ccaggttcca ctggaagacc tctccattgt cggccgggat gtggagatgg gcgtcgaaaa
     9541 tgccgcgccg cgtttctgtc acctcttccg cgctcagcgg gttgcccttg acgaaggccg
     9601 tgagggctac gacggctgag tgtaagaacg tacgcctgtg aaaataagac atgccgcctc
     9661 cagcgtgaca tcagccacgt tccagcatac cgcgccggtt ctatccccag aactggaaaa
     9721 gaagaatcac atagacccag atggcgccca tgaagtgcca gtagtttctg ctgacatcca
     9781 caaaggttct cttagccggg cccaggcgga agcgcagcgc ctcgacctcc acgtaaagca
     9841 ggcagaaaag gccgccggtg agatggatgg catgcgccac cgtgatcatg taaaagaagg
     9901 aactgctggg accggagtta acgtaaaaac cattctcgag cagctcgctc catgccgtgc
     9961 actgaccaaa gaaaaacccg attccaagca acacgcccat cgtccagcaa aaattgaaca
    10021 cttcgcggcg gccactgtga aggcttcgcc gggcccattc gagacaaacg ctacttgcca
    10081 ccagcaggcc ggtgttgatc cacaatatat ccggcaaggg gaggcttacc caatcgtccg
    10141 aagtcccgcg gcgcgcaaac atcgcaacac tgaaggcggc gaagaacatc agaatggaca
    10201 gaagcgcgag aaacatgccg atcaaggcgg cacggcggct cgatccggtt tccggattcg
    10261 gaagctcatg actcctgccg gtcaacggga aaggacctcg cgctcccgcg gaaagcagaa
    10321 acacaccaat tgtgaggata aggccgatcc cagctaaaac cattccctct tttgacaatg
    10381 cccagaaccg gcgtgattgc cggtatgtcg atcgaaacta ccacagctca gggaagccgg
    10441 gcaaatgccg gcgcgtcacc gcgccgcggg tgtttcctcg aagaccttat tccagtcttt
    10501 caggaactgc tccaggcctt tgtccgtcaa cggatggttg aacaacatgt cgagcacttt
    10561 gaatggcatg gtcgcgatat gagctccggc ggatgcggcg tccacgacgt gcagcgggga
    10621 ccgcagcgaa gccgcgagca cctgcgtggc aagaccgtga gtcttgtaga tggacgtgat
    10681 gctgcggacc aggtccattc caatggcgcc aatatcgtcg aggcggccga caaacgggct
    10741 gatgatatat gcgccagcct tggcggcaag cagggcctgc gccggagaaa agcacagcgt
    10801 cacgtttata cgaatgccct ctttgctgag cttggagcag gcagtgattc cgtctcgcgt
    10861 cagcggcagt ttcacaacca cgttcttgtg tattctggag agctcacggc cctccttcag
    10921 catttcgccg gcttccgtag agaccacttc cgcgctgatg tcgccatcca caatgtcgca
    10981 gatggctttg atgtgctcgg cgatcggtct gccttccttg gcaatcagcg acggattcgt
    11041 ggtgacgccg tctacgatgc cccaggcagc tgcttttttc aactctccca gattggctgt
    11101 gtccagaaaa aacttcatgt gattatcctc tcactaccag gttttcgatg gcccccaggc
    11161 ccggaatttc cacgcgaacg acggaaccgg gctgcatggc accgactccc ggaggcgtgc
    11221 cggtagcgat caggtcgccg ggctcgagcg tcatgaactc cgtgacgaac gcgactatag
    11281 catccaccgg aaacatcatc tccgagatgg gcgcgtcctg ctttttcacg ccgtccacat
    11341 aagtttgcac ccgcaattcg tccagccgcg cctccgatgc gggaaccacc cagggtccca
    11401 cagcgcagaa ggtgtcgaac cctttgcccc tggtccactg cccgtctttc ttctgcaagt
    11461 cccgcgccgt gatgtcattc acgcaggtgt agccggagat gtaaccaaat gcttcttcgc
    11521 ggcggacgtg ccgcgcgcgc cggcttatga cgacacccaa ctcgccctcg taatcgacgc
    11581 gcccggaaat gtggggataa acgatgcgat cgccatcggc gattagcgac gagggcggct
    11641 tcaagaaaat catgggttcc gccggcggct cgttgcccag ttccctggcg tgctcgggat
    11701 agttgcggcc cacgcacacg atcttggaag gccagcaggg aggcaggagc cggaccgagg
    11761 acatcgcgtg gctcgctccc ttttccaccg aatcgacgga gggggtttcg aagatcgtac
    11821 cgttttcgac aattccgtat ttcacctcgt tcgacgagtc gggaaccgtc ggctcaatat
    11881 taggattcag actgaagcga attcgtttcg gcataagtta tggtgcggta attggaaccg
    11941 gcgtggagcg ctggctctca tttccaaggc ggtcaaccga agtcaccgcg tactggtagg
    12001 ctttgccgct ctccaccgtg gcgtcgctgt aggccggcgc ctcgacaagg ccggaaattt
    12061 tcgcgaggtc tccgtctccg gccgcgcgat acacgtaata tccctggaga tcgcggttgg
    12121 cgacgcgttc ccaggtgagc tcgacggtgc cggctccggg aatgccaatc acgccggagg
    12181 gcgccgcggg cggaaaggta tcgacgggca cgatagccac tggtggtgaa tgcttgcttt
    12241 ccgcccggat gtctccgtcc tcccgcacgg cttgtacctg gtattcatag cgggagccat
    12301 attcggctgc cgcatcgacc cattccaggg tttccacttc gcccgcttgt gccatctccg
    12361 aagacgcggg cgcgcgccgg tacacacgaa atttcagccc ggccgcacgc tccgggtatg
    12421 cccatgtgac cttggcgcct tcggcggccg attcggcctg aaccttcgac ggcacgggca
    12481 agggagcaat cacgcgaagt gtcacaaggt tcgaccaccc agccgtgcgt ccccgtttgt
    12541 tcaaggcgcg cacacctagc aggatctcct tgccggtgaa ctcttgtgcc ggaacttcga
    12601 tcggctggga cgattcggtg ggctcactcg ggaattcgaa gcgcgttgcc tccgccgccc
    12661 agcgttcgtg gtcaaactcg ccctccccgg ccggaccggc cgccacttct atcgttccgg
    12721 gcttgtcgat tacaaggcct tccgtggtcc tctgcggccg ggtgaaatcc aacaccagca
    12781 tgccccccga ctgtttcaca cgcaaatcca ggatgcgctc gggaatgtta agcgccggag
    12841 gcaacgggtc tcctatggag ccgcaccccg cgagaacaag tggaagcgcc gccgctagca
    12901 gacgccgcat gtcgtcatag gatgtactta ctcagatcct ggtctttgat gatctcggcc
    12961 agttgtttgt ttacataggc ccggtcgatt ttcacggtct tgcgtttcag gtccggaccc
    13021 tcaaacgaga tatcgtccag gagcttttcc atgatcgtat gtagccgccg cgccccgatg
    13081 ttttcggcca tttcgttcac ccgcgccgcg gacctcgcaa tttcttcgag agcatccggg
    13141 gtgaacgaaa gcttgatgcc ttcggtttcg agcagcgcca tgtactgttt gatgagcgca
    13201 tttttgggct cgcgcaggat ccggataaaa tcctgctccg acagtgatcg cagctccaca
    13261 cggatcggga accgcccctg caattcggga atcaggtcgc tcggtttcga gacgtgaaac
    13321 gcccccgccg ccatgaagag aatgtgatcg gttcgcacga agccgtagcg tgtgttcacc
    13381 gtggtgcctt cgacgatggg cagaatatcg cgctggacgc cttcgcggct cacatccgga
    13441 ccgtgaccgg actcgcgtcc ggcgatcttg tccacctcat cgagaaagat gatgccgttc
    13501 ttttccacgc gctcgattgc gatgcgcgtg acctgctcca tgtcgatcag cttctgctct
    13561 tcttcctgga ccagatactc cagcgcttcg ctgacacgca tcttgcgttt tcgcgtgcgc
    13621 ccgccgaaca aaccgggcag gacgtccttg agattgacgt ccatctcctc gataccagcg
    13681 tttgtggaga tctcaaacgt cgggccgcgt tccttcacat cgagttcgac ggtgcgttcg
    13741 tccagtttgc cggtgcgaag cttttccctc agcttctcgc gggttcgctc gatgctgtcg
    13801 ctcgactccc cttgtggggg catcaacagg tccagtacgc gatcttcggc gttgcgctcc
    13861 gcgcggtcgg ccacttcatc caggcgttct tcgcggacca tgtcgatagc gatgtcgacc
    13921 aggtcgcgaa tcatcgattc gacgtcgcgg ccgacgtacc ccacctcggt gaatttgctc
    13981 gcctccacct tcaggaaggg cgaattggca agcttggcaa ggcgcctggc gatctcggtt
    14041 ttcccgacgc cggtggggcc gatcatcagg atgttcttgg gcatcacctc ttcggccatc
    14101 tccggctcga gtttctgacg ccggatgcgg ttgcgcaggg cgacggcgac cgcgcgcttg
    14161 gcttcaggct ggcctacgac gtacttgtcc agctctttta caatttcgcg cggtgtaagc
    14221 tcatcgagga gcggcgcttc atccaccgac tgggtaggca ggtagatgac cattatccga
    14281 gttcctcgta cacaatgttc tcattggtat agatgcagat tttgccggcg atggccatgg
    14341 atttctccgc gatttccctc gcaggcagct ttgtgttttc gatcagggcg gcagcagccg
    14401 ctttggcgaa agggcctccc gagccgatgg cggcgacgtc ttcgtcgggt tcgataacgt
    14461 cgccccctcc gctgacgata taagtgcttt tcaaatccgc cactagcagg agggcttcca
    14521 ggtgccgaag cattttatcg gtacgccaat ccttggcgag ctcgacgaca cttcggggca
    14581 ggtttccgct gtactgctcc agtttggcct cgaaccgggc gaataaggcg aaagcatcgg
    14641 cggtggaccc cgcaaatccg gcaatgatct tgtcgttgta caggcggcga agtttcttgg
    14701 cggaggcttt gaacacctcc tgaccaaacg tcacctgtcc gtcgcccgca agaacgacct
    14761 ttccacccct gcggacacaa aggatggtgg tcga
//
LOCUS       DASTMK010000351        10753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_273690, whole genome shotgun sequence.
ACCESSION   DASTMK010000351 DASTMK010000000
VERSION     DASTMK010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273690"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFQ79_15440"
     CDS             complement(<1..577)
                     /locus_tag="VFQ79_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0141116.1"
                     /translation="MQGPIKITVLGSGTSVGVPTIGCHCEVCSSTDPRNSRLRPSILV
                     STGNMNVLIDTTPDLRQQALRFGIEKVDAILLTHPHADHIMGLDDVRPFNFKKSEPIP
                     IFGSVETIAAIRRSFAYIFDGCETQSSRPKLVTREFDGEPIPIGDARFEPIRLAHGKG
                     VVHGFRFANAAYLTDHSDIPLESLEKLHGLDV"
     gene            complement(578..919)
                     /locus_tag="VFQ79_15445"
     CDS             complement(578..919)
                     /locus_tag="VFQ79_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEU0141117.1"
                     /translation="MSEADTPKPEVTADPEPPETTEQDFPLPPVTFEFLTLSLKMQAE
                     MQLGLIHFGEEKDRPQPEFKLARHTIDLMAMLQEKTKGNLSVDEQRLLENSLTELRFR
                     YIQALEQKQKS"
     gene            993..3839
                     /gene="ileS"
                     /locus_tag="VFQ79_15450"
     CDS             993..3839
                     /gene="ileS"
                     /locus_tag="VFQ79_15450"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU0141118.1"
                     /translation="MYDNGLIPMNLEDVDLKKTVNLPRTDFPMKANLPQNEPRILARW
                     EEEGLYEKIRAARSGRPLYLLHDGPPYANGNIHLGTAFNKVLKDFIVKSKSMAGFDAP
                     YVPGWDCHGLPIEIKVDSELGKKKAGMTASEIRAECRRYASKFVEIHRREFKRLGVLG
                     RWEDPYLTMSAQYESVIAQAFVDVLSQGYVYKGLKPVHWCIRDRTALAEAEVEYENHT
                     SPSIWVRFALTSDPAAIDPALAGRRVYAVIWTTTPWTIPANMAIAFNSRFEYVAAEVG
                     DDVYIVAAELLAETAGKSGWIDPRAIARFRGDRLEGVVFRHPFIERDSPGILADHVTL
                     EQGTGAVHTAPGHGHEDYVIGRQYGIETYCPVSGDGRFFQAEDAPGAIPEEILGKTIW
                     EANPIVVNILNQKGALLAQKTIEHSYPHCWRCHNPTIFRATEQWFIGMDRNELRSRAL
                     EAIRQVKWLPGWGEERISNMIANRPDWCISRQRVWGVPITVFYCDACNEALTDKPVLD
                     RVVKLFAEHTADVWYERTAAELLGDGVVCAKCGGSSFRKESDILDVWFDSGSSHLAVL
                     TEANGLPWPSDMYVEGGDQYRGWFHSSLLIGIALRGASPYRNCATHGWTLDAEGKAMS
                     KSVGNIIEPQNIVNRYGAEVLRLWVASVEFTEDVRISETILTRLSEAYRKMRNTFRYA
                     LGNLADFDPAGDAISPAEMLEIDRWILLRGEKLVRDCRAWYEELAFHRVYQALYNFAT
                     TDLSAIYFDIIKDRLYTAGAASVERRSAQTAIYRLTYALLRLMAPLISFTTEEVWGHF
                     RKPAGAPESVHIAEFPQPDELTASMTERERERFSNWERLMQVRESVMKALELARQEKF
                     IGSGLEAHLRLKANSELYALLNEYERELPALFIVSQASLETEPGVEFSVHVERAEGEK
                     CERCWKYTQDVGSNPQFPSICANCAEVVSELVQA"
     gene            3841..4359
                     /gene="lspA"
                     /locus_tag="VFQ79_15455"
     CDS             3841..4359
                     /gene="lspA"
                     /locus_tag="VFQ79_15455"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU0141119.1"
                     /translation="MNSRAAALSISGAIFVLDRVSKALVEAYVSAWDTYVVIPGFFNI
                     IHTQNRGAAFGLLADAGSAWRTILLVGVSSIVLLFVGALLWRATREGAEESRLSRSAL
                     AMILGGAVGNVYDRVTKGVVTDFLDFYAGTYHWHTFNVADSAITIGASLLLIEMWRQR
                     NEGDRRRNQHAA"
     gene            4370..5161
                     /gene="lgt"
                     /locus_tag="VFQ79_15460"
     CDS             4370..5161
                     /gene="lgt"
                     /locus_tag="VFQ79_15460"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU0141120.1"
                     /translation="MFPKVIQIGDFFLPTYGLLVAIAFLVALWVISRLARRARLNPDA
                     VTNLGIYCALAGLAGAKLFMFLFDWETYVRNPGEIFSLATIQAGGVFQGGLIAALVTA
                     IWYMRHRKLPVLETADVFAPGIALGHAIGRLGCFSAGCCWGARCDLPWAVTFTNPDAH
                     DLTGVPLNVSLHPTQLYEAFAEAIIFIVLYRKFGRAHRSGSILGLYLILYSAVRFTVE
                     FVRFHEQALPFGGPLSITQWIALALLAVGIWLVSRKSPQTVPAAS"
     gene            complement(5198..5883)
                     /locus_tag="VFQ79_15465"
                     /pseudo
     CDS             complement(5198..5883)
                     /locus_tag="VFQ79_15465"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(6017..6868)
                     /locus_tag="VFQ79_15470"
     CDS             complement(6017..6868)
                     /locus_tag="VFQ79_15470"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEU0141121.1"
                     /translation="MRRHIISAESAAPSKTVGSRTAQLTTALYEGGQTTFTHGDVERI
                     TGLPAASARSLIRHAVNRGVVSRLEPGLFVLVPPELGRAVEFAGNPYLVVRQLVAGAE
                     YFISHASAMELHRMVTQPQLVIFTSTTKRLRNRTIHGTDFRFVLTRHENVFGVTTHWI
                     SKQDSVKVSDIERTVIDGLHLPAYCGGVTEVAKGLWMRRDDVKPERLIEYARRLDNGA
                     VVRRLGYLLERYSLTAPEELQRMRHSLSATYAVLDPTLPPEGTHVSGWRLRVNVSPDE
                     LEAVRRT"
     gene            7138..7602
                     /locus_tag="VFQ79_15475"
     CDS             7138..7602
                     /locus_tag="VFQ79_15475"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEU0141122.1"
                     /translation="MKMRCRNERGGALMEGALIFIPLFAMMMAIVDFSFAIFLRSTFQ
                     HAVREGVRYAVTYQTQSGMGHDASIREVVRRQSMGFLNGRENLINIEYCDPATLACNV
                     SNEPGNIVEVSIENYRFGWLASLLRDANPLSITVRASDRMEGLPGGVNPPAR"
     gene            7680..8225
                     /locus_tag="VFQ79_15480"
     CDS             7680..8225
                     /locus_tag="VFQ79_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141123.1"
                     /translation="MPVLLGTFSIGLSLGRSIQASQISRDAGHMYARYVDFSLPGNQD
                     IIVRLARGMGMTRTGGNGIVILSRITFVSQDDCAGSALSPGQCTNADQHVVIHRIVIG
                     NRQLRASAFGTPTPSTINADGSINAPVYLTDLNARAGNFGTLLRLAPGEFAYVAEAFF
                     PSNDLTVPGLQNGGVYARTIY"
     gene            8225..9802
                     /locus_tag="VFQ79_15485"
     CDS             8225..9802
                     /locus_tag="VFQ79_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0141124.1"
                     /translation="MRTQYAQPSTTRRRQRGIALVLTSLLIVFLMAFVGLAIDASLAY
                     AAKARLSAAADAAAIAAARSLNLGLTMAEQEASAIARARAFFAANFPDGHLMTRGLDV
                     DVSVAETAYRTRTVSVSASVNSPAMFMRVLGFDGMTIQAEGRASRRDVNLIIVLDRSG
                     SLQQTDSCEPMKAAARNFVRQFAPGRDRLGLIVFQTGVYRAFAPSMNFRSGSPNLESL
                     ISNIRCAGNTSLAQGLSMGYQELRDLAEPGALNLIVLFTDGLANGLVADFPMRYTSDV
                     RYGDGSGSYSSNSRTYDMLPSPCQWVNPPVHPTEPQSFDENHLGKKFSAPWNPAWVPI
                     AQVATKRGVLTRSGVGSTGNTSGLYQESFSSVSTTSSIPIITDNQGCGFSNAARVRRD
                     IAYIPDQDAFGNATDCCRYNMGTERFTSGPYTGRIRPDKPSAVLKAGANALDSAGRRI
                     RQDDQYNVVVYTIGLGDFDANEPPDETLMRRVSNDPSSPIYDSSRPEGLYVFAPNNTQ
                     LNQAFARVASEILRIAK"
     gene            9847..>10753
                     /locus_tag="VFQ79_15490"
     CDS             9847..>10753
                     /locus_tag="VFQ79_15490"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEU0141125.1"
                     /translation="MAQVFPFQPYRYSASAGSLDTLVTQPYDKISPAMQQRYLSLSPY
                     NLVRIILGQRRDSDNEADNVYTRAAAHLNSWLASGVLVQDKEPAFYAYSQEFEIPDTA
                     ERLVRAGFIGLGAVEEYEAGVVHRHEKTLSGPKKDRMELLRHTRSHFGQVFMLYPDAE
                     NAIDAILGQVMSGPADAEVNDEYGAVHRLWKISDTGRVASIQQLMSDKKLVIADGHHR
                     YETALAFSREHPELRDAQRVMMTFVNMYSPGLRILATHRLLTGLPSFSIGEFQQKAST
                     RFRCTPLKDTGELKARWSEPHENLVR"
BASE COUNT         2089 a         3110 c         3260 g         2294 t
ORIGIN      
        1 gcacgtccaa tccgtgcagc ttttcgaggg actcgagcgg aatatcgcta tggtctgtca
       61 ggtaagccgc attcgcgaaa cggaaaccgt ggacgacacc cttgccatgc gcgaggcgga
      121 tcggctcgaa ccgcgcgtct ccgatcggaa tcggctcccc gtcgaactcg cgtgttacca
      181 gcttgggccg ggaagactgt gtctcgcatc cgtcaaagat gtaggcaaaa gaccgcctta
      241 tggcagcgat ggtctccacc gatccgaaaa taggaatcgg ctcacttttc ttgaagttga
      301 atggccggac atcatccaaa cccataatgt ggtcggcatg agggtgtgtc agcagaatcg
      361 cgtccacttt ctcgatgccg aaccgcaggg cctgctgacg caggtccggt gtcgtgtcga
      421 tcagcacgtt catgttgccg gtgctgacca ggatggaagg acgcaagcgg ctattccgcg
      481 ggtccgtaga gctgcaaacc tcgcaatggc agccgatggt gggtacgccg acactggttc
      541 ccgatccgag aacggtgatt ttgatcgggc cttgcattca ggatttctgt ttctgctcga
      601 gcgcctgaat gtaacggaac cgcagttccg ttagcgaatt ctccagaagg cgctgctcgt
      661 cgacgctgag attccctttg gttttctcct gtagcatggc catcagatcg atggtatggc
      721 gcgccagttt gaactcgggc tgtggccggt ccttctcttc gccgaaatgg atgagcccca
      781 gttgcatctc cgcctgcatc ttaagcgaaa gagtgagaaa ctcgaaagtc accggcggga
      841 gcgggaaatc ctgctctgtt gtctccggag gctctggatc ggcagtaact tcgggtttgg
      901 gagtatctgc ttcactcatt ccactccatt ttactccgtc aaagcggcgc ggtctcctct
      961 tcggccgcgc cgattggtga gcggattgcc gcatgtacga taatggtctt atccccatga
     1021 atcttgaaga tgtggacctg aagaagactg tcaatcttcc ccgcacggat tttccgatga
     1081 aggcgaacct tccccagaac gagccgcgga tcctcgcgcg ctgggaagaa gaggggcttt
     1141 atgagaaaat ccgggccgcg cgttccggcc ggccgttgta cctgttgcac gacggccctc
     1201 cctacgctaa cggcaatatt cacttgggta ccgccttcaa caaggttctg aaagatttca
     1261 tcgtcaaatc gaagagcatg gccggtttcg atgccccgta tgtcccggga tgggattgcc
     1321 acggcctgcc gatcgaaatt aaggtagaca gcgaactcgg caaaaagaaa gccggcatga
     1381 ccgcgtccga gattcgcgcc gagtgccgcc gctacgcttc gaaatttgtc gagatccacc
     1441 gccgcgagtt taagcgtttg ggtgttctgg gccgttggga agatccttac ctgaccatga
     1501 gcgcccaata cgaatcggtg atcgctcagg cctttgtgga tgttctgagc cagggctacg
     1561 tttataaagg attgaagccg gtgcactggt gcatccggga ccgtacggcg ctcgccgaag
     1621 cggaagtgga gtacgaaaat cacaccagcc cgtccatctg ggttcgcttc gcactgacct
     1681 ccgacccggc cgcaattgac ccggcgctcg ccgggcgtcg cgtctacgca gtcatatgga
     1741 cgaccacacc ctggacgatc ccggccaaca tggccatcgc attcaattcc aggttcgagt
     1801 acgtggctgc ggaggtgggg gatgacgtgt acatcgtcgc cgccgaactg ctggccgaaa
     1861 ccgccgggaa atccggttgg atcgatccgc gcgctattgc ccggtttcgt ggggatcgcc
     1921 tcgaaggcgt ggtgttccgg catcccttta tcgaaaggga ttcgccgggg attctcgccg
     1981 accacgtcac gctggagcag ggcaccgggg cggttcacac cgcacccggg cacggacacg
     2041 aagactatgt catcggccgg caatatggca tcgagaccta ctgcccggtg tctggcgatg
     2101 gccgcttctt tcaggcggag gatgcgccgg gtgcgatccc ggaggagatc ctcggcaaga
     2161 ccatttggga agccaatccc atcgtggtta atattctgaa tcaaaagggc gcgctgctcg
     2221 cgcagaagac cattgagcat agctacccgc actgctggcg ctgccacaat cccaccatct
     2281 tccgcgcaac ggaacagtgg ttcatcggca tggaccgcaa cgagcttcgg tcccgcgcgc
     2341 tggaagccat ccgccaggtg aagtggctgc ccgggtgggg tgaggaacgg atttcgaaca
     2401 tgatcgccaa ccggccggac tggtgcatct cgcgccagag ggtctggggc gtaccgatca
     2461 ctgttttcta ttgcgacgcc tgtaacgaag ctttgaccga taaaccggtg ctcgatcgcg
     2521 tggtgaagct gttcgccgag cacaccgccg atgtctggta cgaacgcacg gccgccgagc
     2581 ttttgggcga cggcgtggtc tgcgcgaagt gcggcggcag ctcgttccgc aaggagtccg
     2641 acattctgga tgtgtggttt gattccggat cgagccacct tgccgtattg accgaagcca
     2701 acggactccc gtggccatcc gacatgtacg tcgaaggcgg cgaccagtat cgaggctggt
     2761 tccacagctc gctgctgatc ggcatcgcgt tgcgcggcgc ttcgccgtat cgtaactgcg
     2821 cgacccacgg ctggacgctt gatgccgaag gaaaggcgat gtcgaagtcc gtgggcaaca
     2881 tcatcgagcc gcagaacatt gtgaaccgct acggcgccga agtgttgcgg ctgtgggtgg
     2941 cttcggtcga gttcaccgaa gacgtgcgga tctcggaaac gattctgacc cggctgtcgg
     3001 aggcataccg caagatgcgg aacacgttcc ggtatgcatt ggggaacctg gccgatttcg
     3061 accctgccgg cgatgccatt tcaccggccg aaatgctcga gatcgacaga tggatcctgc
     3121 tgcggggcga gaaactggtc cgcgactgcc gggcttggta tgaggaactg gcctttcacc
     3181 gggtttacca ggccctgtac aacttcgcca cgacggactt gagcgccatc tatttcgaca
     3241 tcatcaagga ccggctctac acggccggag cggcatcggt ggagcgccgg agtgcacaga
     3301 ccgctattta cagactgacc tatgcgctgt tgcggctgat ggcgcccctt atttctttca
     3361 ccaccgaaga ggtttggggt cacttccgca agcccgctgg agcgccggag agcgttcaca
     3421 tcgccgagtt tcctcagccg gatgagttga ccgccagcat gacggaacgc gagcgcgagc
     3481 ggttttcgaa ttgggaacgg ctgatgcaag tccgggaatc cgtcatgaaa gcgttggagc
     3541 ttgcacggca ggagaagttc atcggctcgg gtctggaagc tcacctgcga ttgaaagcaa
     3601 actccgagtt atacgctctg ctgaatgagt acgagcggga gttgcctgca ctcttcattg
     3661 tttctcaagc ttctctagag acggagcccg gcgtggaatt ttcggtgcac gtcgagcgtg
     3721 cggaaggcga aaaatgcgag cgctgctgga agtacacgca ggatgtggga tcgaacccgc
     3781 agttcccctc tatttgcgcc aactgcgcgg aagtcgtcag cgaactggtt caggcctgaa
     3841 gtgaacagcc gggccgcagc tctctcgatt tcgggtgcaa tcttcgtcct cgaccgcgtg
     3901 tcgaaagcgt tggtggaagc gtacgtctcg gcgtgggaca cgtatgtggt tataccgggt
     3961 ttcttcaaca tcattcacac gcaaaaccgg ggcgccgctt ttggacttct ggcggatgcg
     4021 ggcagcgcgt ggcggacgat cctgctggtc ggcgtttcat ccattgtgct tctattcgtc
     4081 ggcgccttgc tttggcgggc gactcgcgaa ggagcggaag agagccggct gtcgcgctcg
     4141 gcgctggcga tgattttggg cggcgcggtg ggcaatgttt atgaccgcgt gacgaaaggc
     4201 gtggtaacgg atttcctcga cttttacgcc ggcacgtatc actggcacac gtttaatgtg
     4261 gcggacagtg cgattactat tggcgcttcc ctgttgttga ttgagatgtg gaggcagcgg
     4321 aatgaaggtg accggcggcg taatcaacac gcggcctgat tggatttgaa tgttccccaa
     4381 agttatccag attggcgact tctttctccc tacctatgga ctgctggtgg cgatcgcttt
     4441 tcttgtcgca ctgtgggtca tttcgcggct cgcgcgccgt gcccggctta acccggatgc
     4501 ggtcaccaat cttggaattt actgcgcgct ggcgggactg gcgggcgcca agctcttcat
     4561 gttcctattc gattgggaga catacgtccg caatcccggc gagatttttt cgctcgccac
     4621 cattcaggct ggaggcgtct tccagggtgg cctcatcgcg gcactggtta ccgccatctg
     4681 gtatatgcgc catcgcaagc tgccggtgct ggagacggcg gacgtgttcg ctcccggtat
     4741 cgccctggga catgccatcg ggcggctggg atgcttctcc gccggctgtt gctggggcgc
     4801 gcgatgtgac ctgccttggg ccgtcacgtt cacaaacccg gatgcgcacg atttaaccgg
     4861 cgtcccgctg aacgtgagcc tgcaccccac acaactctac gaggcgtttg ccgaagcgat
     4921 tattttcatc gtgctttatc gtaagttcgg ccgcgcacac cgttcgggtt caatcctggg
     4981 gctgtatctc atcctgtatt ccgcggttcg atttacggtc gaatttgtga gattccacga
     5041 gcaggccttg ccgttcggcg ggccgctctc gatcacgcag tggatcgcgc tcgccctgct
     5101 tgccgtaggc atctggctgg tatcgcgcaa gtcaccgcag acggtcccgg cggcatcgtg
     5161 attcctaccg cggcctccgg ttgacgagca ggtgcagtca tggcaggttg gcctgccgca
     5221 gtacgcggcg gacctctcgg aagacctggt cgaacggcgg caacacggac atctgattcg
     5281 agagtcgcac ggtccaaagc ttcttcaggc gcgcttcctt cttgatgatg gcatcctgga
     5341 ttccaaccgc ctcttgctgc cggaaggcga gtttggcgct cattgcgggc acgagttccg
     5401 caaggctgat gctttcgcag gtgacgagat gccacaaatc gtagaggtcg cgcggttcgt
     5461 tgcgagcgcc aggactttct cggtcgcagt ttccgcaagc gagtaagcct ggatgacccg
     5521 gccatccggc agatccgaga actcccggta ggcgcgaagt accgggcgag cttcaagagg
     5581 gaattcaagc tgctcgcgga tcgtaatatc caccttgacg gcgccgccgg ctggtaacgg
     5641 tcctacgtac cgcaagtaga atgtgtgact gttcgcatga gaatgacgat cttcgcggtc
     5701 gaatgcgaac tcgattgcgc tggcgtcgcg aacagcggcg tagactggct ccaggttctt
     5761 acgaatgtcc tcaagcggca ttggctgcag gagcgtgaag tcgaggtctt ctgaaaatcg
     5821 gtaatcgcca aagtagcagc gcttgagagc ggtcccacct ttgaacgcga ggctgcgctg
     5881 catgttgctc tcggtcagtt cggcgaggaa ccaggcaagg cagtaatcgc gttcgagcac
     5941 gctttctgga atccggcgcc caccggcctt agcgaggcgg ttggcaagaa gaaagaggtt
     6001 gcgctgtggg atcaggtcag gtcctccgga ccgcctcgag ttcgtcagga gatacgttca
     6061 cacgcaggcg ccaaccggaa acatgcgtac cttccggtgg cagcgtcgga tcgagcacag
     6121 cgtatgtcgc gctgagtgaa tggcgcatgc gctgtagttc ttccggagcc gtgagcgagt
     6181 atcgctcaag taggtaacca agccgccgga cgacagcacc attatccagg cgccttgcgt
     6241 attcaatcaa ccgctccggt ttcacgtcgt cgcgtcgcat ccagaggcct tttgccactt
     6301 cggttactcc cccgcagtac gcggggagat gcaagccatc gatgacggtc cgctcaatgt
     6361 cgcttacctt taccgaatcc tgtttcgaaa tccagtgtgt cgtcacgccg aagacgtttt
     6421 cgtgccgagt gagaacaaat cgaaagtcgg taccgtgaat cgtcctgttc cgcaggcgct
     6481 tcgtcgtgct ggtgaagatg accaactgcg gctgcgtgac catccggtgc agttccatcg
     6541 ccgaggcgtg agagatgaag tactccgcgc ctgctaccag ttgccggacc accaaatatg
     6601 gatttccggc gaactccacc gctcgaccga gttccggggg cacgagaacg aagaggccgg
     6661 gctccaaccg ggaaacgacg ccgcggttga cagcgtgccg gatcaaactc cgcgccgatg
     6721 ctgccggcaa gccggtgatg cgctcaacgt cgccgtgcgt gaaggtcgtc tggccgccct
     6781 cgtagagcgc tgtcgtcaac tgggccgtcc gggaaccaac cgtcttcgac ggcgcggcgg
     6841 attcggcgct tatgatgtgg cggcgcattg gatggccctg ctcctctata ataatggttt
     6901 gtacgcattt cgtgcaatgc catagcgcga attgcgcact aacaataaca aactggccga
     6961 ccccggcggc atcgtgattc ctaccggcta aggtgattct gggtgcgaat ctaagggaaa
     7021 cgcaccggtc ctctcgtctt atttgcctga tttccttcca cccggcgcgc ccgtatgctg
     7081 aggctagcac agaccgcgac catgcgaacg cgggtgtagc ggcaggctgg cgggccgatg
     7141 aaaatgagat gccggaacga gcggggcggg gcgctgatgg aaggggcgct catctttatt
     7201 ccactcttcg cgatgatgat ggccatcgtt gacttcagct ttgccatctt tctgcgaagc
     7261 acctttcagc acgcggtaag agaaggtgtg cggtatgcgg tgacctacca gacgcagagc
     7321 ggcatggggc acgacgcatc gatccgtgag gtcgtgcggc gccagtcgat gggtttcctg
     7381 aatgggcgtg agaacctgat caacatcgaa tactgcgatc cggccacgct cgcgtgcaac
     7441 gtgagcaatg aacccggcaa catcgttgag gtttcaatcg aaaactaccg gttcggatgg
     7501 ttggcttccc tgttgcgtga cgccaacccg ttaagcataa cagtgcgcgc ttcggaccgg
     7561 atggagggac tgcccggcgg cgtcaatccg cctgcaaggt gacgggtatg agaaatcggc
     7621 gcaatcaccg tcggcgaggc cagtcattgg tggaattcgg cttctgcatg gtctttctta
     7681 tgccggttct gctcggaacg ttttctatcg gcttgagcct cggcagaagc attcaggcga
     7741 gccagatcag ccgcgacgcg ggtcatatgt acgcgcgcta tgtggatttt tcgctacctg
     7801 gcaaccagga cattatcgta cggctggcgc gcggcatggg catgacgcgc accggtggaa
     7861 acggcattgt gatcctttca cgtatcacct ttgtcagcca ggatgactgc gccggctccg
     7921 cgctctcgcc gggccaatgc acgaacgcgg atcaacatgt cgtcattcat aggattgtga
     7981 ttgggaaccg gcaactgcgc gccagcgctt tcggcacgcc gaccccgtct accatcaacg
     8041 cggacggcag catcaacgcc cccgtgtacc tgaccgatct gaatgcgcgt gcaggcaact
     8101 tcggcacgct cctccggctg gcgccgggcg aattcgctta tgtggcggag gcgttcttcc
     8161 cgtcgaatga cctgacggtt ccgggtcttc aaaacggcgg tgtctacgcg aggacgattt
     8221 actgatgcgg actcaatacg cgcagccatc tacaaccaga cgccggcagc gagggatcgc
     8281 gctggtgcta acgtcgctat tgatcgtgtt cctgatggca tttgtcggac tggcgataga
     8341 cgcaagcctc gcctatgccg ccaaggcccg gctgtccgct gctgcggacg cggctgccat
     8401 cgccgcggcg cgatccctga acctcggctt gaccatggcc gagcaggaag cgagtgcgat
     8461 agcgcgagcc cgggcttttt ttgctgcgaa tttccccgat gggcacctga tgacgcgcgg
     8521 cctcgacgtg gatgtttctg tcgccgaaac tgcgtaccgg acgcgaacgg tttcggtatc
     8581 cgcgtcggtg aattctccgg cgatgttcat gcgcgttttg ggattcgatg gcatgaccat
     8641 tcaggccgaa ggccgcgctt cgcgccggga cgtaaatctc atcatcgtgc tggatcgctc
     8701 agggtcgctc cagcagaccg actcctgtga acccatgaag gcggctgcca gaaacttcgt
     8761 tcgccagttt gctcccgggc gcgaccgcct gggattaatc gtgttccaga ccggcgtgta
     8821 cagggcgttc gctccttcca tgaacttccg gagcggcagt ccaaatctgg aaagcctcat
     8881 ttccaacatc cgctgcgcgg gcaacacaag cctggcgcaa ggcctctcga tgggttatca
     8941 ggaactgcgc gacctggcgg aacccggagc gttgaacctg atcgtgctct ttaccgacgg
     9001 tcttgcaaac ggtctggtag cggatttccc gatgcgttat acatccgatg tgcggtatgg
     9061 agacggctcc ggcagctaca gctcgaattc ccggacctac gacatgcttc cgagcccctg
     9121 ccaatgggtg aatccaccgg tgcatccgac tgagccgcaa agcttcgacg aaaaccactt
     9181 gggtaagaag ttctccgcgc cttggaaccc ggcttgggtg cctatcgccc aagttgcgac
     9241 gaagcgggga gttctgaccc gcagcggagt tggctccacg ggcaacacca gcgggctcta
     9301 tcaggaatcg ttctcttctg taagcacgac gagcagcatc cctatcatta cggacaacca
     9361 gggatgcggc ttctccaatg cggccagggt acgccgcgat atcgcctata tacccgacca
     9421 ggatgccttc ggcaacgcta ccgattgctg ccgctacaac atgggtacgg aacgattcac
     9481 ttcaggtcct tataccggcc ggatccgtcc cgacaagccg tccgctgtcc tcaaagcggg
     9541 tgccaacgca ttggacagcg ccggccgccg catccggcag gacgatcaat acaatgtggt
     9601 ggtttacacg atcggcctcg gtgatttcga tgccaacgag ccgcccgacg agacgctcat
     9661 gcggcgcgtt tccaacgatc cgtccagtcc catctacgac tctagccgtc ccgaagggct
     9721 gtatgtattc gctccgaaca acacacagct caaccaggcg tttgcgcgtg tcgcgtcgga
     9781 gattctcaga atcgccaaat gagtttcgcg ggatgggatc tggtatccta aaaaatcccg
     9841 cgaaatatgg ctcaggtgtt tccttttcaa ccctaccggt atagtgcttc cgccggttct
     9901 ctcgacacgc tggtcacgca gccttatgac aagatcagtc ccgcgatgca gcagcgctat
     9961 ctgtcgctca gcccgtacaa cctggtccgc atcattttgg gccagcgccg cgattccgac
    10021 aacgaagcgg ataacgttta tacgcgtgcg gcggcgcatc tgaactcctg gcttgcttcg
    10081 ggcgtccttg ttcaggataa ggagcctgcc ttctacgcgt attcccagga attcgaaatt
    10141 cccgacaccg ccgaacgcct ggtgcgagcc ggatttatcg ggctcggcgc tgtcgaagag
    10201 tatgaagccg gtgtggtgca caggcatgag aagacgctgt ccggtcccaa gaaagatcgg
    10261 atggaattgc ttcgccacac gcgttcgcat ttcggccagg tgtttatgtt gtatccggat
    10321 gcggaaaatg cgattgatgc gattctaggc caggtgatgt ccggcccggc ggacgcggag
    10381 gtgaacgacg agtacggggc cgtgcaccgg ctatggaaaa tatcggacac aggccgggtt
    10441 gcgagcattc agcagctcat gtccgataag aagctggtga ttgccgacgg tcaccaccgc
    10501 tatgaaacgg ctctggcctt ctcgcgtgag cacccggagc ttcgcgatgc tcaacgggtg
    10561 atgatgacgt tcgtcaacat gtattcaccc ggtctgcgta ttctggctac gcatcgctta
    10621 ttaaccggct tgccttcctt ctccattggg gagttccagc aaaaggcatc tacgcgtttc
    10681 cgctgcaccc cattgaaaga tacgggggag ttgaaggcgc gctggagcga acctcacgaa
    10741 aatctggtgc ggt
//
LOCUS       DASTMK010000352         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_273720, whole genome shotgun sequence.
ACCESSION   DASTMK010000352 DASTMK010000000
VERSION     DASTMK010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273720"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFQ79_15495"
     CDS             <1..193
                     /locus_tag="VFQ79_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141126.1"
                     /translation="FFFPRVRGTAAFVGVLAGEAAILGASQLTQVSFLWYNVIGALVV
                     IGTGLLVSALWTRNKPVHT"
     gene            complement(184..1437)
                     /gene="glgC"
                     /locus_tag="VFQ79_15500"
     CDS             complement(184..1437)
                     /gene="glgC"
                     /locus_tag="VFQ79_15500"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU0141127.1"
                     /translation="MELRVLAFVLAGGKGTRLFPLTKERAKPAVPFGGRYRIVDFVLS
                     NLINSGIYSIYVLIQFKSQSLLQHIRDGWELSGVLKKHFIIPVPAQMRTPSETWYSGT
                     ADAIYQNINLIEQADPHLVVIFGADHIYRMNIREMIEFHVQKRADVSIAAIPVPKDQA
                     REFGVIEAAADHSVLSFIEKKADAPTMPGDPQRVYASMGNYVFSTGVLLRELYEDARS
                     ENSSHDFGRDILPRLIGQASLYAYDFQTNRIPGDPEDSPAYWRDVGTIDAFYDANMDL
                     RAVSPALNLYNRHWPLRTGSYQDPPAKFTFDEEGRRGQAVDSIVSGGSILAGGIVRNS
                     IIGRNVRIHTGALVDDSVIFDNCDIGRRSKVRRAILDKNVHVPEEDTIGYDLELDRLR
                     HHVTESGIVVVGGARTRVEIAALQV"
     gene            complement(1526..3166)
                     /gene="pgm"
                     /locus_tag="VFQ79_15505"
     CDS             complement(1526..3166)
                     /gene="pgm"
                     /locus_tag="VFQ79_15505"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEU0141128.1"
                     /translation="MPIHPKAGTPATRDMLIDPVKLEQAYCETYPDLEDPNQVVAFGT
                     SGHRGTPGKGTFTEAHIVAITQAICDLRRDREIGGPLFLGKDTHALSAAAQRTALEVL
                     AANQVDVFLQENDGATPTPVISRAILMHNRGRRNDLADGIVISPSHNPPEDGGFKYNP
                     PNGGPAETDITGWVEQRANALLRARNSEAKRWPLARARQASTTYYYDYVTPYVDELKA
                     AIDMDLIGRSGLKIGADPLGGASLAYWEPIAAKYGIDITVVNPHVDVTFGFMTLDHDG
                     KIRMDCSSPYAMANLVRLKDQYAVAFGNDPDSDRHGIVTRSSGLLNPNHYLAAAIDYL
                     LSHRPGWPAGAAVGKTLVSSSMIDRVVAAHHRQLAEVPVGFKWFADGLFNGSYCFGGE
                     ESAGASFLERAGSVWTTDKDGILLNLLAAEITARTGKDPGEHYAELTARFGSPVYRRI
                     DAPATPEQKKALARLTPEAVTAKELAGEPIEAKITRAANGAAIGGLKVQSKNGWFAAR
                     PSGTENIYKIYAESFRGEPHLDAIIEEAQRIVSAAAGV"
     gene            complement(3292..>3833)
                     /locus_tag="VFQ79_15510"
     CDS             complement(3292..>3833)
                     /locus_tag="VFQ79_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU0141129.1"
                     /translation="GQIIRRPCKECGGEGRIRVERKLKVTIPAGVDNGTRLRLPSEGQ
                     PGANGGPNGDLYVFLRVKEHPVFSRRENDLHCTVPINVAQAALGTEIHVLTFDGLESI
                     KVPEGTQSGTEIKLRNKGVPYLNGHGRGDLYVRIDVKIPRKLTREQRKLFEDLLETLP
                     AENEPDEKGLFDKVRDYFA"
BASE COUNT          747 a         1154 c         1092 g          840 t
ORIGIN      
        1 attctttttc ccgcgggtgc gcggaacggc ggcattcgtc ggggttctgg caggcgaagc
       61 agccatcctc ggcgcgagcc aacttaccca ggtctcattc ctctggtaca acgtgattgg
      121 cgccctggtg gtgatcggaa cgggcctgtt ggtttccgcc ctatggactc gaaacaagcc
      181 cgttcatact tgaagcgcag cgatctccac acgagttcga gcccctccca ccaccacaat
      241 tccagactcc gtcacgtgat gacggagccg gtcgagttcc agatcgtatc caatcgtatc
      301 ttcctccgga acgtgaacat tcttatcgag gatggcccgc cgtaccttgg acctgcggcc
      361 gatatcacag ttgtcgaaga tcaccgaatc gtcgacgagc gctccggtgt ggatccggac
      421 gttacggccg ataatggaat tgcggacgat accgcctgcc agaatggagc cgccggaaac
      481 gatggaatcc actgcctgcc ccctgcgccc ttcctcgtcg aaggtgaact tcgccggggg
      541 atcctggtaa cttccggtgc gcaacggcca gtggcggtta tacaggttca gtgcgggaga
      601 aaccgcacgc agatccatat tggcgtcgta aaacgcatcg atagttccca catcgcgcca
      661 gtacgccgga gaatcttcgg gatctccggg aatccggttg gtctgaaaat cgtaggcgta
      721 cagggaagcc tggccaatca gacggggcag gatatcgcgt ccgaaatcgt gtgagctgtt
      781 ctcgctgcgg gcgtcttcgt acaattcgcg gagtagaaca ccggtggaga aaacatagtt
      841 tcccatcgac gcgtatacac gttgcgggtc gcccggcatg gtaggggcgt ccgccttttt
      901 ctcgatgaag gagagaacgg aatgatccgc cgcggcctca atgacgccga actcccgcgc
      961 ctggtctttg ggtaccggga ttgccgcaat cgaaacatca gcccgctttt gcacgtgaaa
     1021 ttcgatcatt tcccgaatgt tcatgcggta gatgtgatcc gccccgaaga tgactacgag
     1081 gtgcgggtcc gcctgctcga tgaggttgat gttctggtag atcgcatccg ccgtgccgct
     1141 gtaccatgtc tcgctgggag tgcgcatctg tgcgggaacg ggaatgatga aatgtttctt
     1201 caggacgccg ctcagctccc aaccatcccg gatgtgttgc agcaaagact ggctcttgaa
     1261 ctgaatgagg acgtaaatcg aataaatgcc ggagttaata agattactca gaacaaaatc
     1321 aacaatccgg tatcgtccgc cgaatggcac ggccggtttg gcgcgctcct tcgtaagtgg
     1381 aaacaggcgg gttcccttcc cgccggccag gacaaaggca agcactcgaa gttccattta
     1441 tcggctccag gtagtgcaca aaccacatcc gtagcaaaca tcattttgtc agccgcctcg
     1501 cttcaaatca acatcgaaat gttttttaaa ccccggcagc cgccgagacg attcgctgcg
     1561 cttcttcgat aatcgcatca aggtgcggct caccccgaaa gctctccgcg tagatcttgt
     1621 aaatgttctc ggtgcctgaa ggacgtgctg caaaccagcc atttttactt tggaccttta
     1681 gcccacctat cgccgcgcca ttagcggcgc gcgtgatttt cgcttcgatg ggctcgcccg
     1741 ccagttcttt cgccgtcacc gcttccgggg ttaaacgagc caacgccttc ttctgctctg
     1801 gagtggccgg agcatcgatg cggcgataga cgggagaacc gaagcgtgcc gtcagctccg
     1861 catagtgctc acccgggtct ttgcccgtac gggccgtgat ctcggccgcg agcagattca
     1921 gcagaatgcc atccttgtcc gtggtccaca cgctgccagc acgctcgagg aaacttgccc
     1981 cggcactctc ttcgcctccg aagcaatagg atccgttgaa caggccatct gcaaaccact
     2041 tgaagcccac cggcacttcg gctaattgcc tgtgatgcgc tgccaccacg cggtcgatca
     2101 tgctgctgct caccagcgtc ttcccaacag ccgccccggc cggccatccg ggacggtggc
     2161 tgagaagata gtcgatcgcg gccgccaggt aatgattcgg attcaggagc ccggaggaac
     2221 gcgttacgat gccgtgacgg tcggaatccg gatcgtttcc gaaggcgaca gcatactggt
     2281 ctttcagcct gacgagattc gccatcgcat acgggctgga acagtccatg cggatcttcc
     2341 catcgtgatc cagcgtcatg aatccgaagg tcacgtccac atgaggattg actaccgtaa
     2401 tatcgatacc gtatttcgca gcgattggtt cccaataagc aagcgaggcg ccgccgaggg
     2461 gatcggcccc gattttgagg ccggaacgcc cgatgagatc catgtcaatc gcagccttca
     2521 actcgtctac gtacggagta acgtagtcgt aatagtaggt cgtcgatgcc tgccgggcgc
     2581 gagcgagcgg ccaccgcttc gcttcgctat tgcgggcccg aagcagagcg ttggctcgtt
     2641 gctccaccca gccggtaata tcggtttccg ccggcccgcc attcggggga ttgtatttga
     2701 aacctccgtc ttcgggggga ttgtgcgatg gactaatgac gatgccatcc gccagatcat
     2761 tccggcggcc gcggttgtgc atcagaatgg cccgggagat taccggagtg ggtgtggctc
     2821 catcattctc ctgcaggaaa acgtcgacct gattcgccgc cagcacttcg agcgcagtcc
     2881 gctgagcggc ggccgagagg gcgtgcgtgt ctttgcccag gaatagcggc ccgccgatct
     2941 ctctgtcgcg cctcaggtcg cagatcgcct gggtgatcgc gacgatatgg gcttctgtga
     3001 aagtgccttt ccccggcgtt ccgcggtgcc cgctggtacc gaaggcaaca acctggttcg
     3061 ggtcctcgag atcggggtaa gtctcgcaat acgcttgctc cagcttcacc gggtcgatca
     3121 gcatgtcgcg agtcgccggt gttccggctt tggggtggat aggcatcttg gctcctctgg
     3181 cgttgtgtcc cggcattgcc gcttcctcgc ggtcgcggct ccgtaagcat tgtgctttca
     3241 acggatccga tgccgagccg cgaccaccag ggagcggttt gagtggctga tttacgcgaa
     3301 ataatctctg accttatcga acaggccctt ttcgtcaggc tcgttttccg caggcagcgt
     3361 ttcgagcaga tcctcgaaaa gcttcctttg ctcgcgcgtg agcttcctgg ggatcttcac
     3421 atcgatacgc acgtaaagat cgcccctccc gtgcccgttc agatagggga cgcccttgtt
     3481 ccgcaacttg atttccgtac cgctctgcgt cccttcgggt accttgatgc tttccagccc
     3541 gtcgaaagtc agaacgtgta tctcggttcc aagcgctgcc tgagctacat tgatgggaac
     3601 cgtacagtga aggtcgttct cgcgcctgga aaagacggga tgttccttca cgcgcaggaa
     3661 cacgtacagg tccccgttcg gtccgccgtt cgcccccggc tgtccttcgg atggcagtct
     3721 aaggcgcgta ccattgtcca caccggccgg aatggtgacc ttcagcttgc gctcgacacg
     3781 gatacggcct tcgccgccgc actccttgca cggccggcga ataatttgcc ccc
//
LOCUS       DASTMK010000353         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_463884, whole genome shotgun sequence.
ACCESSION   DASTMK010000353 DASTMK010000000
VERSION     DASTMK010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463884"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1556
                     /locus_tag="VFQ79_15515"
     CDS             351..1556
                     /locus_tag="VFQ79_15515"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0141130.1"
                     /translation="MARNGRKGRGRWILLVVLVVVLGGAFFGLRAAFKPDNSIDSSKI
                     ATVEMGDIARSVVATGKIEPRSKVEIKSKASGIVKKIYVDYGDRVREGQVLVELDKEE
                     LEARVREMTATLQAAQAAEEAAGAALERNKVEALGPDIPFLKSSWERAKQLDNQGLIA
                     KTLLEDAEKAYQLALNRQMTASRNVAVTRAELAKAKAQVAQARAALERAQEDLRNSTI
                     VSPMDALVLSRDVEVGDAVSSILVLGSQATLVMTLGDVTDVYVLGKVDQADIGKVYLG
                     QRARIVVESFKEKKFEGAVTKISPLGVEKDNVTTFEVRVSIRNPDGTLKANMSANAEI
                     LLEEKKGVLLIPETSVVYDRNRNTSVEVPDAASKNGKRSIPVRLGISNGVKTELVSGL
                     NNGDKLILQ"
     gene            complement(1565..2116)
                     /locus_tag="VFQ79_15520"
     CDS             complement(1565..2116)
                     /locus_tag="VFQ79_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141131.1"
                     /translation="MTAVYLLLAILALGLAYLVFRKPIKTYFRFRGERLIDCPENHAP
                     AAVVVNARRAVMARGQAGLRLKDCSRWPEKQNCGQECLRQIETAPESCLVRNILVNWY
                     AGKSCVLCGKPLDEMNWMEHKPCFLAADGVSYEWSDVPAEALPETFSSHVPICWNCHV
                     ASTFRRKHAELVTDRDWKKTAAK"
     gene            2261..2947
                     /locus_tag="VFQ79_15525"
     CDS             2261..2947
                     /locus_tag="VFQ79_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0141132.1"
                     /translation="MYKDLRITVIIPCLNEEQGVEQVLRRMPSFVDEVIVVDNGSTDR
                     TSEVARSFGAHVIREPVRGYGRSYKKGFAHASGDIIVTLDGDHSYPPDAISYLLEAFL
                     HLEVDFLNASRFPVRDRRAMSFKHYIGNLILSIAMSLLYFRWVRDSQSGMWVFRRSIL
                     KDMVLESDSMAFSEEIKIEALKNPRIRFEEISILYSSRLGEIKLNPWRDGFHNLFFLV
                     RKRFTRRARS"
     gene            2947..3936
                     /locus_tag="VFQ79_15530"
     CDS             2947..3936
                     /locus_tag="VFQ79_15530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0141133.1"
                     /translation="MMGGVTVVIPHWNRHELLASVLADLRTQTYPIDEVLVVDNGSTD
                     GSPEVAEALGAKVVRLPSNRGFAAAVNRGIDECRSELVAILNNDVRLSPEWLHRLVNA
                     LDDPAISFATGKLLNANHKGYLDGAFDLLSRAGCAWRAGNARSDGPSWSTPCRIFSAP
                     LTAGLFRVELFRAIGKLDERYISYMEDVDLGLRCALNGRSGLYVPDAIAWHAGSATLG
                     AWSPDVVRLIARNQVLLTAKHYPGKLVVRWFWAILVGQLLWGVVALRHRALFAFLRGK
                     IAGLASSIPMRQSSARADPVRLREVLEANERNIRELQRQTGFDLYWRIYFGLT"
     gene            3933..4901
                     /locus_tag="VFQ79_15535"
     CDS             3933..4901
                     /locus_tag="VFQ79_15535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0141134.1"
                     /translation="MSKTAILVVTHNSERVIGTCLDACARYSKAPVLVVDNASEDATA
                     AVVRSRTGIRLLANSHNRGFAAAVNQGMAALDAANVLLLNPDVALRTSIDLLEQACEH
                     GAAAAAGLLTDEAGRPQAGFSVRRLPTPWALTFEVMGLNRLWPGNPVNRRYRALDWNA
                     TEPAEVEQPAGAFLMLRRDVWATLGGFDEQFHPVWFEDVDYCKRLRDHGLTIQFVPQV
                     RAFHEGGHSVSRLPWGCRQVYWYVSLLKYASKHFRASERRLVSAAVILGFIPRLVTGI
                     LSGRRAQTIKAFFAVVRFAVPLLLPRGNSGRISPDASPAAGQRVRR"
     gene            4946..>5239
                     /locus_tag="VFQ79_15540"
     CDS             4946..>5239
                     /locus_tag="VFQ79_15540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0141135.1"
                     /translation="MRNYLISVTIVTYNSGRFIKTCLESVLEQKCSEKEIIVVDNGST
                     DGTCDILEQFEDRCRVIYNQENIGFAAAQNQAIRLSSGEWILTLNPDVLLLPNF"
BASE COUNT         1098 a         1468 c         1533 g         1140 t
ORIGIN      
        1 ttcctggtaa cccagctcag aaaagctgaa gatcgagtcc accatctgct gcgtgaactc
       61 gcggcgcgcc tccaccattt cgaccgccgc ctgcttgaga gtttcaagag acgggggctg
      121 cgccgcgaca ggaaccaagc ctataagaga aagaaatagg aaccgtgctt tcatagtcat
      181 ctcgccttcc cacctgtacg caagccggca gaatccctct tggcatgacg tcagggttag
      241 agccaacaat aatatcaggc cggcaggtcg cttatggcgc gcttccggcg gaggctcttt
      301 tttcgatctt tttcgtggcc ttgtgcgtaa caaaagtgag gaatcattct atggctagaa
      361 acggaaggaa aggccggggc cgctggatcc tgctggttgt cctcgtcgtc gtgctcggcg
      421 gtgccttttt cggcctgcgg gcggcgttta aaccggataa cagtatcgat tcatccaaaa
      481 tagcgactgt cgagatgggc gacatcgccc gatccgtggt cgcaacgggc aaaatcgaac
      541 cgcgctcaaa ggtagagatc aaatcgaaag ccagcggcat tgtcaaaaag atctacgtcg
      601 actacggaga tcgtgtcagg gagggccagg tcctcgtcga attggacaaa gaggaattgg
      661 aagcccgggt tcgtgagatg accgccactc tccaggcagc ccaggcggcc gaggaagccg
      721 cgggcgccgc attggaaagg aacaaggtag aagcgctagg cccggatatt ccttttctga
      781 agtcctcctg ggaacgcgcc aaacaactcg acaaccaggg gctgattgcc aaaacgctgc
      841 tcgaagacgc cgagaaagcg tatcaactgg ccctgaatag acagatgacg gcatcgcgca
      901 atgtggcagt cacccgggcg gaattggcaa aggcaaaagc ccaggtcgca caggcccgcg
      961 cagcgctcga acgggcccag gaagatttgc gtaactcgac gatcgtcagt cctatggatg
     1021 cgctggtgct ttcccgcgac gtggaagtgg gcgacgctgt gagctccatc cttgtacttg
     1081 gctcgcaggc cactctggtg atgacgctgg gagatgtgac cgacgtctat gtgctgggaa
     1141 aggtggatca ggccgacatc gggaaggtgt atctcgggca acgggcccgc atcgtcgtcg
     1201 agtccttcaa agaaaagaaa ttcgagggcg ccgttaccaa gatttccccg ctcggcgtgg
     1261 aaaaggataa cgtcaccaca tttgaggtgc gagtctctat acggaacccg gatggaacgc
     1321 tgaaggcgaa catgagcgcc aatgccgaga tcctgctcga agaaaagaag ggggttctgt
     1381 tgatcccgga gacttccgtg gtctacgacc gcaatcggaa cacttctgtt gaggttccgg
     1441 atgccgcgag taagaacggg aaacgcagca taccggtccg cctcggtatt tcaaatggcg
     1501 tcaaaacgga actggtttcc ggactgaata acggcgataa actgatcctg caataggact
     1561 ccggctactt cgcagccgtc ttcttccaat ccctgtccgt caccagctcc gcgtgttttc
     1621 gccggaaggt cgatgcgacg tggcagttcc aacagatggg aacgtggctg ctaaatgtct
     1681 ccggcaacgc ttcggccggt acatcgctcc attcgtaact cacaccgtct gcggcgagaa
     1741 aacagggttt gtgctccatc cagttcattt cgtccagcgg cttcccgcag aggacgcagc
     1801 ttttgccggc gtaccagttc acgagaatat tccgtacgag acagctttcc ggcgcagtct
     1861 caatctggcg caggcactcc tgcccgcagt tctgcttttc gggccaccgt gagcagtctt
     1921 tcaggcgcag gcctgcctgt ccacgcgcca tcacagcgcg cctcgcattc accacgacgg
     1981 ctgccggcgc atggttttcc gggcaatcga tcaggcgttc cccgcgaaac ctgaaatagg
     2041 ttttgatggg ttttcgaaag accaggtaag ccaatccgag ggccaggatg gctaacagaa
     2101 gatatacggc ggtcatactg agtcgcctcc ttgatttact tccttgcaat agccggacca
     2161 acctataact cctccggcaa ccagtgctta caccaatcgc ttcagattct tctggggctt
     2221 atgcctcacc ggctggggtg ttttccctac aatagttagc ttgtacaagg acctgaggat
     2281 taccgtcatc attccatgcc tgaacgagga gcagggggtc gaacaggtat tacggcgcat
     2341 gccttctttt gtggacgagg tcatcgtggt cgacaacggg tcgacggacc ggacttccga
     2401 ggtggcacgc agttttggcg cacacgtcat tcgtgaaccg gtccgcggtt acggcagaag
     2461 ctacaagaaa ggtttcgccc acgccagcgg ggacatcatc gtaactctgg acggcgacca
     2521 tagctacccg cccgacgcta tttcctacct ccttgaagcg ttcctgcacc tggaggtgga
     2581 tttccttaac gcatcgcgct tccctgtccg tgaccggcgt gccatgagct tcaagcacta
     2641 catcggcaat ctgattcttt ccatcgcgat gtccctgctt tactttcgat gggtacggga
     2701 ttcgcaatcg ggcatgtggg tcttccgccg gtcgattctg aaggacatgg tgctcgagtc
     2761 cgacagcatg gcattttccg aggagatcaa aatcgaggcg ttaaagaatc cacgtatccg
     2821 tttcgaagag atctccatcc tctattcatc acggctcgga gagatcaaac tgaatccctg
     2881 gcgggacggt tttcacaacc tttttttcct ggtccgcaag cggtttactc gaagagcgag
     2941 gtcctgatga tgggcggagt aacagtggtc attccgcact ggaaccgcca tgagctcctt
     3001 gcttccgttt tggccgatct gcgaacacaa acgtatccga tcgacgaagt gctggtagtg
     3061 gacaacggct ccaccgacgg atctcccgaa gtggccgaag ccctgggcgc caaagttgtg
     3121 cgccttccct cgaaccgagg tttcgccgcc gcggtgaacc ggggcattga cgagtgccgc
     3181 tcggagctgg tcgccattct aaacaacgat gtccgtctgt ccccggagtg gctgcaccgg
     3241 ttggttaatg cactcgacga tcctgcaatt tcctttgcga ccggaaagct gttgaatgcg
     3301 aaccacaagg gatatctgga tggcgcgttc gatctgctct cgcgggcagg ctgtgcgtgg
     3361 agggcgggga acgcgcgttc cgacggccca tcatggtcca cgccgtgccg gatcttttcc
     3421 gctccgctga cggcaggcct gtttcgtgtc gagctcttcc gggccatcgg gaagctcgat
     3481 gaacgctaca tctcgtacat ggaagatgtt gatctcgggc tgcggtgcgc gctgaacggc
     3541 cgttcgggcc tatatgtgcc ggatgccatt gcctggcatg ccggcagtgc gacgctgggc
     3601 gcctggagcc cggacgtggt ccgtctgatc gctcgcaacc aggtcctgct gaccgcaaaa
     3661 cattatccgg gcaagcttgt tgttcggtgg ttttgggcta ttttagtagg acagcttctc
     3721 tggggtgtag tggccctgag acaccgcgca ctcttcgcat tccttcgtgg aaaaatagcg
     3781 ggactcgctt cttcgattcc gatgcggcaa agcagtgccc gcgcggaccc tgttcgcctc
     3841 cgcgaagtgc tggaggcgaa tgaacgaaac atccgtgagc tccagcgcca aacaggcttc
     3901 gatctctact ggcggatcta cttcggattg acatgagcaa aaccgccatc ctcgtggtga
     3961 cacacaattc cgaacgtgtg atcggcacgt gtctcgatgc gtgtgcgcga tattcgaaag
     4021 ctcccgtgct ggtggtggat aacgcttcgg aagacgccac ggccgctgtg gtccgttcga
     4081 gaaccggcat ccggctgctc gccaacagtc acaaccgggg ttttgccgcc gcggtgaatc
     4141 agggcatggc ggcattggat gcggcgaatg ttctgttgct gaatccggat gtcgcgcttc
     4201 ggacttcgat tgatttattg gagcaagcct gtgaacacgg cgcggcggca gccgccggct
     4261 tgctgacgga cgaagcggga aggccacagg ccggtttctc cgtgcggcgg ctgcctactc
     4321 cttgggctct cacttttgaa gtgatgggac tgaaccggct gtggcccgga aatccggtga
     4381 accggcggta ccgtgcgctc gactggaacg caaccgaacc cgccgaagtg gagcaacccg
     4441 ccggcgcgtt cctgatgctc cgccgcgacg tctgggcaac attaggaggc tttgacgagc
     4501 agtttcatcc tgtgtggttc gaggatgtcg attactgcaa gcgtctgcgc gaccacggtc
     4561 tcaccatcca attcgtgccg caggtgcgcg ccttccatga agggggccat tccgtctcgc
     4621 gcctgccatg gggatgccga caggtgtact ggtatgttag cctgctaaag tacgcgtcca
     4681 agcacttccg tgcctccgaa aggcgtctgg tgagtgcggc agttatttta ggatttattc
     4741 cccgcttggt cacggggatc ctttcgggcc ggagggcaca aaccataaaa gctttttttg
     4801 cagtggtaag gtttgctgtg ccgttgctgt tgccacgtgg aaattcaggc cgtatttcgc
     4861 ctgatgcttc cccggcagcc gggcagcgag tccggcgatg agaggacgcg gttaacggtc
     4921 ccggtcgaaa aaaaactcac aataaatgcg caactacctg atctcagtca caatcgtgac
     4981 ttacaacagc ggccgtttta ttaagacatg tttggagtcg gtactggagc agaagtgttc
     5041 ggagaaggag atcatcgttg tagataatgg atctaccgac ggcacatgcg acatcctcga
     5101 gcagttcgag gaccgctgcc gtgtgatcta caaccaggaa aatatcgggt tcgcggcagc
     5161 gcaaaatcaa gccattcgcc tgtcctcggg agagtggatt ctgacactca acccggatgt
     5221 tctgctgctg cccaatttc
//
LOCUS       DASTMK010000354         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_530009, whole genome shotgun sequence.
ACCESSION   DASTMK010000354 DASTMK010000000
VERSION     DASTMK010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530009"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VFQ79_15545"
     CDS             <1..1765
                     /locus_tag="VFQ79_15545"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEU0141136.1"
                     /translation="MRSWAPILLSAAGLAPALVAQTSVSLEPSVASPAAVGTLVTWTA
                     TVSDGANPGTLWYRFIIRGFDPRPRIVKDFGPDNSFRWAAGEAEGFYAIEVRVRNRDT
                     GEQMEAVAPFQVVSNVTSGQPVAKTTSHPLVWLYSAPPCPLGASMFVYFQAAGVEPGT
                     ADALHRTPAKQCNGLFSMNFHIAGIRPATPYHVKHVIERNSLFSEGPVLQFTSGEAPD
                     HLPVHTVSEGSFTSSNQRVFLAGSLFTNFVATDLEGNLIWYYPEVITFLTRPEPGGYF
                     FGINEDESGDQSKQIVRMFDLEGITVFETNAARVNEQLTAAGKRPINAFHHEARRLSN
                     GHILVLAGVEQIMQNVQGSDSANILADMIIVLNRNLEVVWTWDAFDHLDVQRAATMDD
                     MCTPGVCPPLFLAPEAKDWLHSNSVAETPDGNLLLSIRSQDWVIKIDYQNGQGSGNVL
                     WRLGKDGDFRIDSSDPDLWFSHQHDAEYESDGTLTIFDNSNVRQAANPEANSRGQVYR
                     LDEQNRVAELVMNADLGYYAFALGSAQRLENGNYLFDAGFLLDGAGISLETDANGNTV
                     HAIKSSAPEYRTFRLKDMYTP"
     gene            1775..3166
                     /locus_tag="VFQ79_15550"
     CDS             1775..3166
                     /locus_tag="VFQ79_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0141137.1"
                     /translation="MVPDRNGSRRAFLKGAALAAMGVSLRAQSRPPNIVLIYADDLGY
                     GDLSCYGSGIATPNLDRMAEEGVRFNHFYSASSVCSPSRAALLTGRYPTRVGVPRVLG
                     PEDNSGIPSSETTIAQMLKTAGYRTMCVGKWHLGSRPEFMPVNRGFDEYFGIPYSNDM
                     WPRPLMRNNDVIERPATLETLTERYTLAAEDFIARSQDSPFFLYMPHTAPHIPLAVSP
                     RFTGGSGHGAYGDTVQELDWSVGRILETLKERGLDENTLVMFSSDNGPWYQGSAGRLR
                     GRKGDSYDGGMRVPFIARFPGRIPSGQTSNALATTMDVLPTVARLTGASLPAAPLDGI
                     DIWPLMTNVTDNLPREVFLYFNDIYLQCARLGPWKLHMARYNVPMFTPVPERGRCNLP
                     LPYPELYNLVADPTEAYDRARRNPSVVNEIRERVERLIRTFPDDVFYAYSDTQRRKVH
                     DTPADALPIEKID"
     gene            complement(3191..>3708)
                     /locus_tag="VFQ79_15555"
     CDS             complement(3191..>3708)
                     /locus_tag="VFQ79_15555"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0141138.1"
                     /translation="GVRIVNASADHTGLEDACASLVYGEAMLSMQTPEQKRRILAEAF
                     RILKPGGRYGIHELALLPDSIDIAVRKTIEREMSMNIHVGVRPATFSEWKELMEEAGF
                     KVTWQTTVPMHLLEPKRVLADEGLGGVLRIAFNLLRRPKARRRVLSMRRMFRRYASNL
                     TAVAMVCEKPA"
BASE COUNT          777 a         1100 c         1049 g          782 t
ORIGIN      
        1 aatgcgttcc tgggcaccga tattattgag cgcggcaggg cttgctcccg ctctggtagc
       61 ccagacctca gtgtcactgg aaccgtcggt cgcttcacct gctgcagttg gaactttggt
      121 cacatggacg gcaacggtat ccgacggcgc gaaccctggt acgctttggt accgctttat
      181 tatcaggggc ttcgatccgc ggcctcgtat cgtcaaagat ttcggtccgg ataatagctt
      241 ccggtgggct gccggagagg ccgaaggctt ctacgcgatc gaggtccgtg ttcgaaacag
      301 agacacaggc gagcaaatgg aggcggtggc cccttttcag gtcgtttcga acgtgacttc
      361 cggccagcct gttgcgaaaa ccacgtctca cccactcgtg tggctttaca gcgcaccgcc
      421 atgtcccttg ggtgccagca tgttcgtcta cttccaggcc gccggagtag agccgggcac
      481 cgcggatgct ctccatcgca cgcccgcgaa acaatgcaac gggctcttca gcatgaattt
      541 ccatatcgcc ggaatccggc cggccactcc gtatcatgtc aagcacgtta tcgagcggaa
      601 cagcctgttc tccgaagggc cggtgctgca attcacgtcc ggagaagcgc cggaccatct
      661 tccggtgcac accgtaagcg agggatcgtt tacttcgtca aaccaacgtg ttttcctcgc
      721 gggctctttg tttaccaact ttgtcgcaac cgaccttgag gggaacctga tctggtatta
      781 ccccgaggta attacttttc tcacccgccc ggaacccggc ggctacttct tcggaatcaa
      841 cgaagacgaa tccggagacc aatcgaagca gatcgtacgc atgttcgatc tggagggaat
      901 cacggtattc gaaacgaacg cagcgcgagt caacgagcag ttgacagcag cgggaaaacg
      961 tccgatcaac gcatttcacc atgaggcccg gcggttgtcg aacggtcaca tcctggttct
     1021 ggcaggcgtg gagcaaatca tgcagaacgt ccaggggtcc gattccgcaa acatcctggc
     1081 ggacatgatt atcgtattga atagaaacct ggaagtggtc tggacctggg atgctttcga
     1141 ccaccttgat gtccagcgag cggccaccat ggacgacatg tgcactccgg gagtctgccc
     1201 cccgctgttt ctggcgccgg aagcgaaaga ctggttgcac tcgaactccg tggccgaaac
     1261 acccgacgga aatctgcttt tatcgattcg cagccaggac tgggttatca agatcgacta
     1321 ccagaatgga caaggctcgg gaaatgtgtt gtggcgcctt gggaaagacg gggatttccg
     1381 aatcgactcc tcggaccccg atctctggtt ttcccaccag cacgatgccg agtatgagtc
     1441 cgacggcaca ctcacaatct tcgataacag caatgtcaga caagccgcaa atccggaagc
     1501 caatagccgc gggcaggtat acagactcga tgaacagaac cgcgtagcag aactggtgat
     1561 gaatgccgac ctgggctact acgctttcgc gctcggctca gcccaaagac tggaaaacgg
     1621 caactatttg ttcgacgccg ggttcctgct ggacggcgcc ggtatctcgt tggaaacaga
     1681 cgctaacggc aatacggtcc atgccataaa gagctccgct ccggaatacc gtacttttcg
     1741 gttaaaagac atgtatactc cataacaaag ggttatggtt ccggatcgta acgggagccg
     1801 gcgcgcgttc ctgaaaggcg cggcgctggc ggcaatgggt gtgtccctcc gcgcgcagtc
     1861 gcggccgcca aatatcgtat taatttatgc cgatgacctc ggctatggag atctgagttg
     1921 ttatggatcg ggcattgcga cgccgaacct ggataggatg gcggaagaag gagtgcggtt
     1981 caaccacttc tattccgcca gctccgtctg ttcgccatca cgcgccgcat tgctgacagg
     2041 ccgttatccc actcgggtgg gtgtgccgcg ggtgcttggt ccggaggata attccggaat
     2101 tccatcgtcg gaaacgacga ttgcccagat gctgaagacc gccgggtaca gaaccatgtg
     2161 cgtcggcaaa tggcatctcg gctcccggcc ggagttcatg ccggtcaacc gcggctttga
     2221 cgagtatttc ggcattcctt acagcaacga catgtggccg cggccgctga tgcgaaacaa
     2281 cgacgtcatc gagcgcccgg cgaccctgga gacactgacc gaacgctaca cgcttgccgc
     2341 ggaggatttc attgcccgct cgcaagattc gccattcttc ctttacatgc cgcacaccgc
     2401 gcctcacata cccttggcgg tatcgccacg attcaccggc ggttccggcc atggtgccta
     2461 tggcgacacg gttcaggagc tcgattggag tgtgggccgt attctggaga cgctcaagga
     2521 gaggggcttg gacgaaaata cgctcgtaat gttctcgagc gataacggcc cgtggtatca
     2581 gggcagtgcc ggcagattac gcggccgtaa aggcgacagc tacgacggcg gaatgcgcgt
     2641 tcccttcatt gcaaggttcc ctggccgcat tccatccggg cagacctcga atgccctggc
     2701 caccacgatg gatgttctgc cgacggtagc gcggttgacc ggggcatctc ttcctgcggc
     2761 tccgctggac ggtatcgaca tctggccgtt gatgacgaac gtcaccgata atttgccccg
     2821 tgaagtgttc ctctacttca acgacattta cctgcaatgt gcacgcctgg gtccgtggaa
     2881 gctgcatatg gcccgataca acgttccgat gttcacaccg gtcccggaac gcggacgctg
     2941 caatctgcca cttccttacc cggaacttta taatcttgtt gccgatccca ctgaggccta
     3001 cgatcgcgcc agaagaaacc ccagtgtggt caatgaaatt cgcgagcgcg tggaacggct
     3061 catccggact ttcccggatg acgttttcta tgcctattcg gacacccagc ggcggaaagt
     3121 gcatgatacc cccgccgatg cccttcctat cgagaagatt gactgatttc cgcggcgggg
     3181 ccgattccta ttacgccggt ttctcgcaca ccatcgccac ggcagtcagg ttggaagcgt
     3241 agcgccggaa catccgccgc atcgagagaa cacgccggcg cgccttcggc ctgcggagca
     3301 ggttgaaggc gatacgcaag acgccgccaa ggccttcatc ggccagtact cgcttcggct
     3361 cgagcagatg catcggcacc gtcgtctgcc atgtcacttt gaagccggcc tcttccatga
     3421 gttccttcca ttcgctgaag gtggcgggcc gcacaccgac gtggatattc atggacatct
     3481 cgcgctcgat tgttttgcga acggcaatgt cgatcgagtc cggcagcagc gccagttcgt
     3541 ggatgccgta ccgtccaccc ggtttcagaa ttcgaaaagc ctcggccaga atacgccgct
     3601 tctgctcggg cgtctgcatg gatagcatcg cttcgccgta caccagagaa gcgcacgcgt
     3661 cttcgagacc cgtgtgatcc gccgatgcat tcacgattct cacgccgt
//
LOCUS       DASTMK010000355        28896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_498049, whole genome shotgun sequence.
ACCESSION   DASTMK010000355 DASTMK010000000
VERSION     DASTMK010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 28896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28896
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498049"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFQ79_15560"
     CDS             complement(<1..365)
                     /locus_tag="VFQ79_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141139.1"
                     /translation="MTGDSAIPAIRFRAMRMLGYLLWIASAAFAVIALAAWFSLMDGW
                     IRFLASTRQSLGFAAPPGPDWLLIAGLAAGIAVPVVALIGIYAVYMRSWRRRLSVVWA
                     GVPVRASYTRVRPLEPVAA"
     gene            464..982
                     /locus_tag="VFQ79_15565"
     CDS             464..982
                     /locus_tag="VFQ79_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141140.1"
                     /translation="MAEGTGFTTVYRSADASAKEDAEAVYDLLSSAGMNAVLLDEKAP
                     GVFSGAYEVRVPPERATEAEQLIAARVPAGERPVDTSRDLDLETIFSAQGTTAEMEAI
                     GVKSILEANLIPTVLIGNSALPVLRFEVRVARECADAAKRAIAEARAAGPEAAEEAQR
                     EAEDTALGGSGI"
     gene            993..2831
                     /locus_tag="VFQ79_15570"
     CDS             993..2831
                     /locus_tag="VFQ79_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU0141141.1"
                     /translation="MPYQPIEDYGVVGNMHTVALAGRNGSIDWFCYPNFDSPSVFAAI
                     LDHQKGGYFRIYPLEEELTRKQLYWPGTNVLVTRFLSVHGVAEIVDYMPVKATHDGVG
                     YHGLIRRLKMIRGEMRFRMECFPAFDYGRAKHRVDVHDNTARFRSGAVNLGLEADFPL
                     QREGDGVTAEFVLNNGGTANFELHGISGADDAEIGISDAEAERLFRETVQFWRDWLSQ
                     CTYRGRWREVVERSALVLKLLTFAPTGAIVAAPTTSLPESVAGPRNWDYRYTWIRDAA
                     FTLYALMRIGFTGEAAAFMAWVDDRCRHLEPDGSLEVMYAIDGSHVPEEIDLDHLEGF
                     RHSRPVRIGNGAHSQLQLDIYGELMDSVYLFNKYGVQIPYEQWTNLRRLTNWLCDNWR
                     RKDEGIWEVRSGAQHFVYSKMMCWVAIDRALRLADKRSFPADRERWLKVRDEIYEDIL
                     QNGWSEERQAFVQYYGSDSLDASALMMPLVFFLSPGDPKMLKTLNAINRPPSERGLVS
                     NGMVYRYNIEETPDGFATGEGTFNICTFWLVEAMTRAGAADRRYLEEARLLFEQMLSY
                     ANHLGLFSEQTGPRGEALGNFPQAFTHLALISAAFNLDRSLGASGR"
     gene            complement(2869..4140)
                     /locus_tag="VFQ79_15575"
     CDS             complement(2869..4140)
                     /locus_tag="VFQ79_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141142.1"
                     /translation="MQPIDRPALERIRPEDIFQQIDLICGSHSFRNRESFRKLLTYLA
                     GKTLDGSAEYLKEYTVGIELFGKPENYDPREDAAVRVAISKLRQKLEEYYVGEGKDDS
                     VVIELPRRQLALTWHVRQPAFDAMGRQLPAVPARPHVERPSPWRLIGAAAVLCVVAFL
                     TGRQFAPTPAASVPVEVGQFWRPLLADGRPVLISLGTPMFVRMRGARVRAGHEDIASA
                     QEDPRIRQIQTLFDSPDTQASYIYTGVGEAAAAFRLAKLFSRWNVDLRLRRNSALTWE
                     DIGNNNLIILGSAKYNPKIRNLPVEQSFLVEQGGVTNLHPGTGEPAAFRRRYADDPER
                     SIIEDFAVISRLPGVNGRGVIVAVGASATEGTAAAVDYMTDPARLRELFGHLKGASGE
                     VPKYFEAVLRVEFREMVPVRTDYRTHREVRP"
     gene            4230..5762
                     /locus_tag="VFQ79_15580"
     CDS             4230..5762
                     /locus_tag="VFQ79_15580"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0141143.1"
                     /translation="MSELTRRSVLGAAASAVVTAGRPQPTGGHRPNILFICSDQHSGA
                     ALGANGHGLVRTPNLDRLAAGGVNFRNAYCGSPVCVPGRASLMTGKFASDVGSYCNST
                     PYNGSEGTWGTRLRQAGYDCWATGKMDLWLGRDLGFREVGTSHGHSQNPDVTSLFRAP
                     VCFRPGERDNVDGSFSHRRAPDQPKIDAALAFLRQESKQTGRAWCAYVGLSKPHPKWN
                     AAASYEGMYPPSEMPLPSWPEGYLERRHIAFQILANFKNIQVPISKARIRLARAAYYA
                     CISEVDELIGSLLAELERTGQRENTIVVYTSDHGEMLGDHGLWLKNVLLENAVRIPMI
                     VAGPGIPKGRTVETPVSHVDLVATMLDLAGASVASGLRGHSLIPAARGENGSHPGFAY
                     SESHSEGNCTGSFLIRKGDWKYVYFTGGEPLLFNLKEDPGEFRNLAADTKSAGIREEL
                     HAHLCSLVDPDSVTRASFRKQQSVLKQLVAGNSRQEFYRRLAGRLGPLQARMITERHY
                     TT"
     gene            5921..9340
                     /locus_tag="VFQ79_15585"
     CDS             5921..9340
                     /locus_tag="VFQ79_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141144.1"
                     /translation="MNRHSLIGIVPIAVLVCVAHAQEFRGSISGTVTDASGGSVPGAT
                     VTAINTSTNVRLPTTATSTGSFTIPFVLPGNYRIAVEAAGFKTAIRESIDVEIQQRVR
                     LDFELEPGVVTESITVSAETPLLDTADASLGEVVNRRQMIDLPQAGRNAYLQARIAPG
                     IMPTDTRLFARVFDNGAVSNVSISGAPRRSNDLLLDGIPNADSTNTVAFVPTVEAVEE
                     MKAQTNTYDAEFGRAAGGTINITVRSGTNQFHGSMLEFLRNDKLEANSFFNNRSGQPK
                     PRQRYNQFGATAGGPVYIPKLYDGRNRTFIFGSWESIRQKDPEAILTTVPTLLERNGD
                     FSQSFDASGRLLPVYNPLTTRPDRDRPGRFIRDPFPGNAIPASLQDPVARKLMSYYGE
                     PNQPGARLTSVENFAWSGSSPDRYDSLLLRGDHNFTDRQRIFVRTSTSVRPRTGNNLF
                     DNIASTPATSERISRGAALDYLNTLTSSLMLNLRYGFTRYGDSDDFQDFSIAELGMPA
                     SLAAAVSNAHFPDIGISGYEGLGDSGDSRTRQNVHSFQANMTRIGSSHNLKWGFDHRM
                     YQANYQDTGNASGSFSFNPNFTRGPDPVSNTNTGHAVASFLMGTPSSGSIDNNVQPAF
                     FNKYYGLYLQDDFRVTRSLTLNLGLRYEYETPRMERHDRMVRGFAYDTPSPIAIPGRQ
                     LLGGLQFAGAGGEPRYQWNPQRTNFAPRFGIAWQIDPRVVFRAGYGIFRAGASDFGGG
                     TAASPGFSATTPMVTSLDGVTPVHRLSNPFPDGLLAPIGSSEGLRTLTGQSIAFVDVN
                     GRVPYTQQFSAGFQVQTPGNFVIDASYSGSRGTGLSIGNVNINQLPVEELARGGALQQ
                     SVPNPFFGQFASGVLASANTTLGQLLRPYPHFNSVTTRNPTVGNSTYHAALLKVERRM
                     ANGLTFLASYTVSKLIDDVGAPQNNYNIRGERALSVFDRPQRLVVSGLYELPFGPGKA
                     LTGGSSPIVHRIIGGWQLNWISTFMSGAPLAVTSQVNTTGSLGGSQRPDSTGVSPALE
                     GPNRSRLDRYFDTSQFRAAAPFTFGNLSRQLPDVRGPGLHNWDISLIKNTNIAETVRL
                     QIRGEAFNAFNLPAFDNPATSFGASTFGRISAVQNRANPARQIMLGMKLLW"
     gene            9351..10295
                     /locus_tag="VFQ79_15590"
     CDS             9351..10295
                     /locus_tag="VFQ79_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0141145.1"
                     /translation="MKRSIFVFCALLGGFAAGQHDAAIEFIHVTDTHVTDLKQVSPPL
                     VKAREHFSDGERRLAALLAGTGRPSTASFALITGDLIDAFSYEGRSADRVFGQLDAFR
                     RATARSSIPLYLALGNHDIAHYGLAPDGVKPMADQSVAAAARAAWTRSMECFRDGTYY
                     EFSKQSGSTRYVFLMLDNGYTAAGAAAGQQGVRIAHEQLYWLRRRAAANRDAIIVLAM
                     HIPLGKDGTSEAIRSAVSEAPNIGLILAGHNHRDQVEELVLGASTAVQVRTAALGYGA
                     NNCRRIRLLPDRIEIYKTGAHEELERTVVAGRTVRPAA"
     gene            10431..14102
                     /locus_tag="VFQ79_15595"
     CDS             10431..14102
                     /locus_tag="VFQ79_15595"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141146.1"
                     /translation="MKRAIFLISAFAVAHPAWAQESRGSIAGVVRDQQGASIVGATVV
                     VTNNETNAANRTTSNASGYFEASFLNPGGYTVSVEATGFRRLVRSGLTLSVGSRLDLD
                     FQLEIGQVAETVEVTAEAPLLDTSSAGGGRVIDQQQILQLPIADMNPFTLAALAPGMQ
                     WTGNPGFRRPSDVGGTSSFNTMGGVGQNEYTIDGSPVTGTSRRVGFVPPSDAVTEFKL
                     EANNFDASYGHSTGAAINVATRSGTNRYHGTLYDQHWQQRWNATPHFTRLQFENAVRR
                     GEKSPGDQRQGSGRSNFFGGNLGGPVRIPKLYDGTDKLFFFFSYNGHYERTWDADSPN
                     RTVPAQAWRDGDFSDLLAIDAVRYTIYDPRSARLVNGRVVRTPFPGNRGVPVFNPLYD
                     YYTSLYPQPNNVPGLVSPEGFDNYFADRTVNTLKYNAIVNRVDYNINERNRVSGRWQY
                     SRRTPNTRDWAYETRPGLLAVGSHRVNKGAAGNYVWVLNNNTLLDVSASWARFADGSR
                     NDMRNSIKPSDVGLPAYMDEKAGDFFTLPAIQFSELETLSTTYPMLTASGTTGEFRLG
                     VTTLKGGHSLKMGYTERRYWFASADPGYSSGIFTFNNAYTRQADDTNTASQHGLEWAA
                     FRMGLPSSVSIDTVDSAYWSTRVRSLYVQDDWRLTNRIRINLGLRYEREGGITERFNR
                     GLSGEFLHDVRLPFSEIAESVYARNPLPELPAAQFQVAGGTSYLGQLKDTFTNGTHRL
                     LPRFGFVAEIANSTVLRAGYGWYFDTLNANNTQPSQHGYSLPTATVITNDTGLSFTGV
                     GPAANLSPANNLMLDPFPVRADGTRFNVPYGNRLGTSARAGTSHTFFARDYAPAAQQR
                     WKLGVQRQLMRDLVVEISYNGAFSRIPVNQPINFLPREYWATGNVRNSARDTDLNANV
                     PNPFRVTNMPSLATSEPLLYQYLSTVSFFTSSNIRKNQLLRPYPHMTTLNGVRPGGSF
                     EDTRGRNWYNDLQLMVERRFSRGFQSALMYTYASSAVQDVYLHEFDSQPSWQPNNSVR
                     PHRFVWSAIWELPFGRGRKWVQNNPIQYVIGGWQLSWVYQYQSGPATTWGNRFFYGDM
                     ANLKDLLNADEAHGRDIHLWFDPSISYRGTGVIPTNFQGFEGRSAAQPGQYHVRVFPT
                     RLSILRADGIRNWDVKILRKFRVTEGLSASFSLDMLNATNHTNFSAPNTDPTNTNFGR
                     VTSQNGLSRRLQLNLRIEF"
     gene            14102..15325
                     /locus_tag="VFQ79_15600"
     CDS             14102..15325
                     /locus_tag="VFQ79_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141147.1"
                     /translation="MRLTGFALWFLAAVAAAQTAGEVLRPWSPGTLDIHHINTGKGDS
                     ALLVFPDGTSMLVDAGNTLRPGPRVTPQRPGNTRTPGEWIARYVRHMLEPTGIVRLDY
                     ALLTHFHEDHMGELSQAARRSAGGYQLTGITEVGDAIPIATMLDRGWPDYAFPGPVTG
                     PMMTNYREFLKWQVQHRKMRVERFEAGRNNQIGAVREPGRYRDFEVRNIAVNGEIWTG
                     VGSATRAHFPPIDSVPKDNLPAENMCSAGLRVSYGKFDYFTGGDMPGLPAEGSPPWHD
                     VETPVAKAVGPVDVHTLNHHGFHDCCNAFFLGALRPRVHIMSVYAPSHPGMRVLRRLQ
                     STRIYPGPRDVFATNLMQETKVVIGDALNRLKSTQGHVLVRVAPGGASYRVVILDDSA
                     ETYRVTAIHGPYESR"
     gene            complement(15412..16425)
                     /locus_tag="VFQ79_15605"
     CDS             complement(15412..16425)
                     /locus_tag="VFQ79_15605"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0141148.1"
                     /translation="MAVDGAPARRLAYSVALSAAVAVAAGIIAPLIGSAPLSYSRAFA
                     GQSPDFEILFYARVPRVLLSLIAGGGLAVAGVLFQAVLRDALATPFTLGISAGASLGA
                     VITISAGIGTIAAVPATWFGAALGAFTTLLIVLGVATEGRRMSSFTLLLTGTTVNSIS
                     MAGILFLQNLSTFGQSFAIVRWLMGGIEAVNYSTLAWIGSLVVAASCVLFFKAREWNV
                     LAVGEEWAEARGVSTSRLLLTGYLAGSLIAGSVTALTGPIGFVGLIVPHALRLRLGAD
                     HRILLPCAFFLGAAFLAVCDVVARTLLAPSEIPVGVITAMLGGPFFIYLLRSRRKAAV
                     SGG"
     gene            complement(16415..17134)
                     /locus_tag="VFQ79_15610"
     CDS             complement(16415..17134)
                     /locus_tag="VFQ79_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0141149.1"
                     /translation="MLEGIHAGFTAPELVAIAGPNGAGKSTMLGIMAGLRGEYEGRCL
                     YGGTEVLRWRRRLFAREVSFVPQNLRLEFPFTAEEVVLMGRTAHENGLFESPDDFKAV
                     ERAMRITDTLAFRERDFRSLSGGERQRVILASALAQEPRTLLLDEPTTFLDIRHQLAI
                     YELLRELCRTRGLLIVAVTHDLNLAAAYADRIVMLNRGRIVIDAPPERVISAKIIADV
                     FGVAAEISHTRAGRPWIVYGS"
     gene            complement(17185..18033)
                     /locus_tag="VFQ79_15615"
     CDS             complement(17185..18033)
                     /locus_tag="VFQ79_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEU0141150.1"
                     /translation="MRALIAFLVAAVLWAAPPARIVSTAPSITETLYALGLGPRVVAV
                     TAYCRYPPEAARKPKIGNFLNPNLEVILGLRPDLVIIHRNPTGLAGRLQNMGLKTLEV
                     DQQTMADVFTSMHAIGAAAGVPEAARAVEKAIRAQLEEVRRKTAKLPRPRTMFLAGRT
                     PGAIEGMVAAGGGSYHHELIEIAGGKNIFESVTGAYPRVSLEEVLSRDPEVIIDMGEM
                     SDPAGVTEERKRAVVALWRKYPVLSAVRNGRVYAIASDIFVVPGPRMVEAARAFAAMI
                     HPEAGF"
     gene            complement(18033..20267)
                     /locus_tag="VFQ79_15620"
     CDS             complement(18033..20267)
                     /locus_tag="VFQ79_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141151.1"
                     /translation="MHELSFAGIVIIAFCAPLFSQAPIQGTVFDPTGRRVQGAVIECQ
                     DRRATSGFDGTFRIEGPDECKATVTRSGFEEQQIDLKAGAGNRVDLDLAGVVERVVVT
                     ATRSQTTAEAAGVAVNVITAGEIKTQQSPMVADLLRELPGIHVVRSGRYGALSAVFTR
                     GAQRTGTLVLIDGVPMNDPGGELNFGHLTSSSLERIEVVRGPESALFGAEASAGVIQL
                     FTRRGDPERTVPHGTFSYERGTFQTDRWVAGLSGGSGERLDYSLNAEQFHSVGEFTND
                     YYRNTTGSANVGYRFSPATEFRGTYRSFDAALGVPGQVHYGLVDHDAHETARDSLVSA
                     RIHDARGSRFVQQFGFGYHRQKDVFTDFGMDGPYELALLVRDVRTPVPRVYREAILDA
                     RNLPSTRPPGTRLITQSVTLYPFDPPFVTLHSRKEFQYQGNLAHTGGSFVFGYDYERQ
                     QGNVSERDVARNNHGVFLHEQYTIGRRVFLSGGIRIENSDVFGTKVAPRGAVSVQVAG
                     EHGPLTSTFLRASAGRGITEPSLVQNFSRTAFYVGNPDLRPERTNSYEAGVVQEWFGR
                     RLRTEVAAFHNSFNDLIVFVSLPPPVFGSWDNVERSWARGLEFSGRARLASDLHVSGA
                     YTRLYTRITHSNDPSSLFTGIGQELARRPRNSGSLSISFAPRRWSGFVGCALVGERQD
                     TDFLGVSRNSGYQNVYAGGSWNLHSHVTPFLRVDNLLNSRYEEALGYSSLSRTFRGGV
                     QLGW"
     regulatory      complement(20290..20494)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(20554..21915)
                     /locus_tag="VFQ79_15625"
     CDS             complement(20554..21915)
                     /locus_tag="VFQ79_15625"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0141152.1"
                     /translation="MSGGRVLVAEDDARARDALVALLEEEGYDVECAVDGIAASECVA
                     AGDFDAVLLDVRMPGKDGLSLLREWRDWGRAPAVLVMTAYGTSAVAIEAMKLGAYDYL
                     TKPLHFEEVLIQLERAIAARRQTAALRRYGEVAEEEQNEAEIVGNSPAMQQVYKLIGQ
                     VAPTDSTVLIRGESGTGKELVARALHRHSPRRQRRLITVNCAAIPETLLEAELFGYER
                     GAFTGAVSQRKGRFELADGGTVFLDEIGELSPSTQAKLLRVVQERTVERLGSEQTFTV
                     DVRIIAATNRDLEAAVRGNSFRQDLYYRLNVFSIWLPPLSKRREDIPELTWRLLKKLS
                     SQRKLPLSGITPEGVQALCQREWPGNVRELEHTLERALVLSRGAPITPEHLAPETMED
                     PADPFGSVSLEEGMHTLVSQLERRLIERALAQAGGNRTRTAEILKINRRLLYDKLREY
                     GIE"
     gene            complement(21912..23375)
                     /locus_tag="VFQ79_15630"
     CDS             complement(21912..23375)
                     /locus_tag="VFQ79_15630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0141153.1"
                     /translation="MRISLDSFRAQIAVTIFASLSMLALAWLLAEDILTSTRERLVAE
                     AREQCIRAAEELSQQYRDRANLSNEPVEVLPFEALDVSLRGLSLAVLRAYEGMAGGFY
                     LAEQRRMAGDVPPGGLESQQTLAKFERDVAHSAIVRIGDPGETVVEVASLGRDVYVAA
                     AHRADSGAVAWTLKRLAEARDPVLERRRWWLAALVFATVLGLGGIISIMLYLRRGVGS
                     VNEGLRRLEEDFAYRMPAIGGQFGAIAQAINRMSDRRGALETRLRRQDRLAALGRVVS
                     GVAHEIRNPLNSIRLTLELLERRARRGALRGDEAREAIEEVDRLEQILGRLLEFGKTG
                     LEERRRQNVRPLLDRAIKLVHEQAQSKQIQVRLDTEAGAPLEAEVDGLQLEQVLINLL
                     LNAIDASPSGEEVRISAGCDGDNLRIRVEDCGPGIPPDARDHIFDPYFTTKDAGTGLG
                     LAMSREVVLRHGGTLEFDTGPEGTVFTLAVPSREAPL"
     gene            complement(23476..23982)
                     /locus_tag="VFQ79_15635"
     CDS             complement(23476..23982)
                     /locus_tag="VFQ79_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141154.1"
                     /translation="MAEFSERLKRLESDGGVTSEQRFLHERVTELMAKWKPLDAGTYT
                     DSRIRSAIDSFLDASEELKGARKQSRSSRSESADGDARRRTARVLERTYFRVKQGEYF
                     SAQSKDPFGPEYVRLGRRLYQQARSAYDRGTFELARRLAEASHEVIEGLEKLAQAAVP
                     IPMPPPLD"
     gene            complement(24084..26627)
                     /locus_tag="VFQ79_15640"
     CDS             complement(24084..26627)
                     /locus_tag="VFQ79_15640"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141155.1"
                     /translation="MRYLIVLLLAGWSVQAQSVSTRIQGHRVSGSVTDSTGLPVPGVK
                     ITLHTGGETPGARSTETNVEGKFSFENLPTGEARLEARSPGFSDSQQEIEITRDISNL
                     QIRLELAGRSEEVTVTAQSDEPELSPTGNADALELDSEMLYGLPVLDQDILAAAGEFV
                     DDSAMGSEGVSLVVDGMETDQLGVSPSAIAEVRINRNRYSAEFSRPGRGRIEVITKQG
                     SDQFHGSFMAGLRDARMNARNAFSPTRPPQQRRFFEGHLTGPLTGSGNTTFLLSVERQ
                     TDELQQPVFARTPSGLLQQSLVQPERETGIYGRVNRYVGERHVLSWRYGYDREWQKQG
                     IGGFNLPESAYENRDRDHVAGFGYRWFPSATFFSDLEIRYRNSHETNTSATPDAARIV
                     VNDAFTGGGAQQDQAQDRNRLSLNWVFSNTRGRHSLRWGIMTPTLEWQRLVDRNNFGG
                     TYQFASLDDYVTGRPLSYTVRVGQPDVSFFYWQGSAFLQDDIKLSSRLTLGLGLRYDA
                     QGHLGDYNNIAPRLSIAWGLNESGKTVIRAGGGMFYDRLGMGTVSDLLQLNGLRLRDL
                     QILAPAYPILDIDGTAALPPNIVELSRRAKLPYSFDFSGGIEHRFTRDFQVSANYSVT
                     RGTSLWRTIDANAPLPPDFERPMDSYGFIRQIQSSGTMKSQGLHISANGRMLRFFRGQ
                     VRYRLGSSHNDTGGLPPDSLDLRSQWARADWDRRHRFRAAGLVDLPRSFGLGIIYSAS
                     SGRPYEWTTGLDLNRDGLTLERPNGVGRNALQMPGESRLDLRLSRAFRLSGGASERPP
                     AVVLSLDGFNVLNRVNYTRLVGNQSSPFFGEPVAASAARRLQASVRFTF"
     gene            26780..27190
                     /locus_tag="VFQ79_15645"
     CDS             26780..27190
                     /locus_tag="VFQ79_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0141156.1"
                     /translation="MRTLLAIAGVMTSFAAAGQEVENAEKAWAAAVAANDYAAIDRLT
                     GDDLIYGHSTGLVETKSQYVAALRSGQQKYDQIRHESMNVKTHGNTAVVSSRVRMTGS
                     TKGKPFDNQLIMMHVWVKNGGAWKLVGHQTARMP"
     gene            27212..27784
                     /locus_tag="VFQ79_15650"
     CDS             27212..27784
                     /locus_tag="VFQ79_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141157.1"
                     /translation="MKTLAQFRQQAVFLRESSGSTNIEKTLLKALKVLKLFNIPHYVC
                     GGFAVQERGYPRFTTDVGIIVPDVSFAIEKLSINGFKQNLGSNMTVTDRETKVEVDLL
                     PGGQKIDPGPLTLPIPTHVSDKPQILTLEKLISAKLSTYMGRGIERSQDYADVVKLVQ
                     ANHLPRDFGVDPRVRDEYHKIWDGLHQKDI"
     gene            complement(27818..28027)
                     /locus_tag="VFQ79_15655"
     CDS             complement(27818..28027)
                     /locus_tag="VFQ79_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141158.1"
                     /translation="MAIRFDQLIRPSMVIRDVKVQYPQTVEVFENLRFRDSCDDCSIE
                     VVARKHGLDSRLIIDALNEAAFGVK"
     gene            28286..>28896
                     /locus_tag="VFQ79_15660"
     CDS             28286..>28896
                     /locus_tag="VFQ79_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HEU0141159.1"
                     /translation="MESGNRVHVHQGCSEPEELVAALMRRAPELTDVEIVHCATMGTA
                     DYTRPEFEGHFRHAAFFIGGNVREAVQCSRADYIPIFLSEIEALFESGAMPIDVSLLQ
                     CTPPDDYGYMSLGAGVDIALTAARYSKHVIVEVNDQCPRTFGDCFIHVRDVDAMVECS
                     HPLPEYRSHEVTPADEKIAAQIANLIPDGATIQTGIGAVPDCVL"
BASE COUNT         5842 a         8969 c         8536 g         5549 t
ORIGIN      
        1 tccgcggcga cgggttcgag cgggcgaacg cgcgtatagg aagcccgcac cggcactccg
       61 gcccatacga cactcaggcg tcttcgccaa ctccgcatgt aaacggcgta gatcccgatg
      121 agagccacga cgggaacggc gattcccgcg gcgagtccgg ctatgagcag ccaatccggg
      181 ccgggcggag cggcgaaacc gaggctctgg cgcgtggacg cgagaaaacg aatccagcca
      241 tccatgaggg agaaccaggc ggcaagcgcg atgacagcga atgccgctga ggcgatccac
      301 aaaaggtagc cgagcatccg catagcccgg aagcggatag cgggtatcgc actgtcgcca
      361 gtcacgattg gcgtctcctt gcttgagggt aagcgtttgt gagcggcgcg ggcaagcccc
      421 aaccgtgcat gcgtttcgcg ggtggtagcg gtatgctcta gttatggcag agggaacagg
      481 cttcaccacg gtatatcggt cggccgatgc aagcgcgaag gaagatgccg aggctgttta
      541 cgacctgctc tccagcgcgg gcatgaatgc cgttctgctc gacgagaagg ctcccggtgt
      601 cttcagcggc gcctatgagg tccgtgtccc tcccgagcgc gcaaccgagg ccgaacagtt
      661 aatcgcggcg cgcgtccctg ccggggagcg gccggtggac acctcgcgcg atcttgacct
      721 cgagacgatt ttcagcgccc aaggaaccac cgcggaaatg gaggccatcg gggtgaagag
      781 cattctggag gcgaacctga ttcccaccgt gctcatcggc aatagcgcgc ttcccgttct
      841 tcgcttcgag gtacgcgttg cgcgcgagtg cgccgatgcc gcgaaacggg ccattgcgga
      901 agcgcgcgcc gccggacccg aggccgccga agaggcacag cgcgaagccg aagatacggc
      961 gctgggcggt tccgggatct gaccggtgcg gaatgcccta tcaacccatt gaggattacg
     1021 gtgtcgtcgg caacatgcac accgttgcgc tcgccggcag gaacggatcg atcgactggt
     1081 tctgctatcc taatttcgat tcaccgagcg tctttgcggc gatactggac catcaaaaag
     1141 gcggttactt ccggatctat cccctcgaag aggagttgac ccggaaacag ctctattggc
     1201 ccggtactaa cgtccttgtt acacgatttc tatccgttca cggcgtggcg gagattgtcg
     1261 actacatgcc cgtgaaggcc acccatgacg gggtcggcta tcacgggctg attcgacgtc
     1321 tgaaaatgat ccgcggcgaa atgcgctttc gcatggagtg ttttccggca ttcgactatg
     1381 gacgcgcgaa gcaccgtgtc gacgtccatg acaacacggc ccggttccga tccggcgccg
     1441 tcaatctcgg actcgaagcc gattttccgc ttcagcgcga aggcgacggc gtgaccgccg
     1501 agtttgtgct caacaacggc ggaacggcga actttgagtt gcacggtatt tccggcgccg
     1561 acgacgctga gatcggaatc tccgatgccg aggcggagcg ccttttccgt gagacggtgc
     1621 aattctggcg cgactggctg tcgcagtgca cctatcgcgg cagatggcgc gaggttgttg
     1681 agcgctccgc gctggttctc aagttgttga ccttcgctcc caccggcgca atcgtcgctg
     1741 cgccgacaac cagcctgcct gaaagtgtcg ccgggccgcg caattgggac taccgctaca
     1801 cctggattcg cgatgctgcg ttcaccctgt atgccctgat gcgcatcggc tttaccggcg
     1861 aggctgcggc gttcatggca tgggtcgacg accggtgccg ccacctggag ccggacgggt
     1921 ctctcgaagt gatgtatgcc atcgatggca gccacgttcc ggaagagatc gatctcgatc
     1981 acctggaagg attccgccac tcccgtcccg tccgcatcgg caacggagcc cacagccagt
     2041 tgcagctcga tatttacggc gagctgatgg attcggttta tctgttcaac aagtacggag
     2101 tgcagatccc ctacgagcag tggaccaatc tgcggcggct gacgaactgg ctttgcgaca
     2161 actggcgccg gaaagatgag ggcatctggg aagtccgcag cggcgcgcag catttcgtgt
     2221 actcgaagat gatgtgctgg gtagcgattg accgcgcttt gcggctggcc gacaaaagat
     2281 cgttccccgc cgatcgcgaa agatggctga aggtccgcga cgagatttac gaagacatcc
     2341 tgcaaaacgg ttggagtgag gagcggcagg cgttcgtgca gtactacgga agcgactccc
     2401 tggacgcatc ggcgctcatg atgccgctgg ttttcttcct gtcgccgggg gatccgaaga
     2461 tgctgaagac cttgaatgcc atcaaccggc cgccgtcgga gagaggtctg gtctcaaacg
     2521 ggatggtcta ccgctataac atcgaggaaa cgccggacgg gttcgcaacg ggagagggga
     2581 cattcaatat ttgcaccttt tggctggtgg aagcgatgac cagggccggc gcggcagatc
     2641 gccgttacct ggaagaagcg cgcctgttat tcgaacagat gctgagttac gcaaaccatc
     2701 tgggactttt ttccgagcag accggaccac gcggggaagc gctcgggaac ttcccacagg
     2761 cgttcaccca tctcgctttg atcagcgccg ctttcaatct cgaccggtcc ctgggcgctt
     2821 cgggccgcta gtatcccgac ctggagattc gatttatgac tcgggacatt agggtctgac
     2881 ctcacgatgc gttcggtagt ccgttctgac cggtaccatc tcgcggaact cgacacgcag
     2941 gacagcttcg aaatacttcg ggacctcacc ggatgcccct ttcagatgcc caaagagctc
     3001 gcgcaacctg gccggatccg tcatatagtc tacagccgcc gccgtgccct cggttgcgct
     3061 ggctcccacc gcgacgatca ctccacgccc attcactcca ggcaaccggc tgattaccgc
     3121 aaaatcttcg ataatcgaac gctcggggtc gtccgcgtag cgccggcgga acgctgcagg
     3181 ttcgcccgtt ccgggatgca gattggtcac ccctccttgt tcaacgagga acgactgctc
     3241 aacgggaagg ttgcggatct tgggattgta cttcgcggat ccgaggatga tcaggttatt
     3301 gttcccgatg tcctcccagg taagcgcgga attacggcga aggcgcaggt ctacgttcca
     3361 acgagagaat agtttcgcga gtcgaaaggc cgcggcagct tcgccgactc ctgtgtagat
     3421 ataggacgcc tgtgtgtccg ggctgtcgaa aagagtctgg atctgcctga tcctgggatc
     3481 ctcttgcgcg ctggctatat cctcgtggcc cgcccgaacc cgcgcaccac gcatccgaac
     3541 aaacatcggt gtgcccagcg agatgaggac ggggcggcca tcggccagaa gcgggcgcca
     3601 gaactgcccc acctcaacag ggacagaggc ggccggggtt ggcgcgaact gccgtccggt
     3661 cagaaacgca acaacacaaa gaaccgccgc tgccccaatg agtctccaag ggctcggccg
     3721 ttctacatgc ggccgtgccg gtacagccgg caactggcgc cccattgcat cgaatgcggg
     3781 ctggcgaacg tgccacgtta gcgcgagttg gcggcgcggc agttcaatga cgacggagtc
     3841 gtccttccct tcgccaacgt agtactcctc gagcttctgc cgtagcttgc tgatcgccac
     3901 ccgaaccgct gcatcctcgc ggggatcgta gttttcgggc ttaccgaaca actcgatacc
     3961 gacggtgtac tctttcaggt attccgcgct tccgtcgagt gtcttcccgg ccaggtaggt
     4021 cagcagttta cgaaaagact cgcgattgcg gaaggaatga ctgccgcaga taaggtctat
     4081 ctgttggaag atatcttcgg ggcggatccg ctcaagcgcc ggcctatcaa tgggttgcat
     4141 cgcgagacta catgtagtct acacctttta gacatgttta acttgatttc gcttggcccg
     4201 tggagcaaac ttagccaaat tttatattta tgtcagagct cacccgccga tcggtgctcg
     4261 gagccgcggc ttccgcggtc gtcacggctg gacgtccaca gccaacgggc ggacaccggc
     4321 cgaacatttt gttcatctgc tccgaccagc actccggagc ggcgctcggg gcgaacggcc
     4381 acgggcttgt ccgcacccca aacctcgacc ggcttgccgc cggaggcgtg aacttccgga
     4441 atgcctattg cggcagcccg gtatgcgttc ccggacgcgc ctccctcatg acggggaagt
     4501 ttgcatccga cgtgggttcg tactgcaaca gcactcccta caacgggagc gaaggaacat
     4561 ggggaacccg cctgcggcag gccggatacg attgctgggc gacgggcaaa atggacctgt
     4621 ggctgggacg ggacttgggg ttccgcgaag tcggcacgtc ccacggacat tcgcagaatc
     4681 ccgacgtcac atcgttgttt cgcgctccgg tttgcttccg cccgggcgag agggacaacg
     4741 tcgatgggtc cttctctcat cgtcgcgcgc cggaccagcc gaagatcgac gccgctctgg
     4801 cgttcctccg gcaggaatcc aaacagaccg gcagggcctg gtgcgcttac gtggggctga
     4861 gtaagccgca cccgaagtgg aacgccgccg cgagctacga gggaatgtac ccgccatcgg
     4921 agatgcctct gccgtcatgg ccggagggtt acctcgagcg gcgtcatatc gccttccaga
     4981 tactcgcgaa tttcaagaat attcaggtcc cgatctccaa ggcccggatt cgcttggcga
     5041 gggcggctta ttatgcctgc atctccgagg tggatgaact catcggcagc ctgctggcgg
     5101 agttggagcg gacgggccag cgcgagaaca cgatcgtcgt ctataccagc gatcacggcg
     5161 agatgctggg cgaccatggt ctctggctca agaatgtgct gctcgagaac gccgtccgga
     5221 ttccaatgat cgtcgccggg cctggtatcc cgaaaggacg aaccgtcgag acgccggtgt
     5281 cgcacgtcga cctggtcgcg acaatgctcg atctcgccgg cgcaagcgtt gccagcggct
     5341 tgcgcggaca ctcgctgata ccggcggcgc gcggcgaaaa cggcagccat cccggtttcg
     5401 cgtatagcga atcgcattcg gagggcaact gcacgggttc gttcctcatc cgcaagggcg
     5461 attggaagta cgtctatttc accggtggcg aacctctgtt gttcaacctg aaagaggatc
     5521 ccggcgagtt tcgtaatctc gcggcggata caaagagcgc cgggattcgg gaagaactgc
     5581 acgcgcatct ttgttccctg gtcgaccccg acagcgtcac acgagcttcg ttccgcaaac
     5641 agcagtcggt actgaagcaa ctcgtagccg ggaactcgcg acaggagttt tacaggcgcc
     5701 tggcgggccg gttggggccc ctacaggctc gcatgattac cgagcggcat tacaccacat
     5761 aaaatggcac ccactggtga aaagaaaccg cgagcttttt caaattggga ttttttaccg
     5821 ccggttgcgg cggcgcgcgg cgcggaccgc gtcaaaaacg ccgtcttgtt tattcggctt
     5881 ttcggatacg ccgcagggaa attcaagata gggggttggt atgaatcggc attcgctgat
     5941 agggatagtc ccgatcgcgg tcctggtctg cgtcgcgcac gcgcaggagt tccgcggatc
     6001 gatatcgggc acagtaacag acgcatccgg cggctcggtt ccgggagcca ctgtcacggc
     6061 cataaacacg tccacaaatg tccgtctgcc aacgacggcc acatcaaccg gctcatttac
     6121 aattccattc gtgctgcctg ggaattaccg catcgcggta gaagccgcgg gtttcaagac
     6181 ggcgattcgc gaatctatcg acgtcgagat tcagcagcgg gtccgtctgg atttcgagtt
     6241 ggaacccggc gtcgtaacgg agagcatcac ggtcagcgcc gagaccccgc ttctcgacac
     6301 ggcggatgcc tcgcttggcg aagttgtgaa ccggcggcag atgatcgacc tgccgcaggc
     6361 aggcagaaac gcttaccttc aggctcgtat cgctccggga attatgccga cggacacgcg
     6421 cctgtttgcg cgcgtcttcg ataacggcgc cgtgtccaat gtgtctatta gcggcgcgcc
     6481 gcggcgttcc aacgacctgc tgctggatgg aattcctaac gcggattcga ccaacacggt
     6541 cgcgtttgtg cccacggttg aagccgtcga ggagatgaag gcacaaacga atacatacga
     6601 cgccgaattc ggacgtgccg ccggaggcac aatcaatatt accgtgcgct cgggaacaaa
     6661 ccagtttcat ggctcgatgc tcgagttcct ccgcaacgat aaactggagg ccaacagctt
     6721 cttcaacaat cgcagcggac agcccaagcc gcgtcaacga tataaccagt ttggcgcgac
     6781 tgccggcggc cccgtataca ttccgaagct ctatgacggc aggaaccgca ccttcatttt
     6841 cgggtcctgg gaaagcatcc gccagaaaga tccggaagcg attcttacga ccgtgccgac
     6901 cctgctcgaa cgcaacggcg acttctcgca gtcgttcgac gcgtcgggac ggctattacc
     6961 ggtctacaat ccgttaacga cacgccccga tcgggacagg ccgggccgct tcatccgcga
     7021 tcctttcccg ggcaatgcga ttcccgccag cctgcaggat ccggttgcac gtaaactgat
     7081 gagttattac ggcgagccta accagccggg cgcccggctc acctcggttg agaatttcgc
     7141 ctggtccgga agttcgccgg accgatacga ctccctgctc ctgcgcggcg atcacaactt
     7201 caccgaccgc cagagaatct tcgtgcgcac ctctacaagc gtgcgcccgc gcacgggcaa
     7261 caacctattc gacaacatcg cttcgacgcc cgcgacctcc gagcgcatca gccgcggcgc
     7321 agcgctcgac tacctgaaca cgttgaccag cagcctcatg ctgaatctcc ggtacggatt
     7381 tacccggtac ggcgacagtg acgactttca ggacttctcc atcgccgaac tcggtatgcc
     7441 ggcatcgctc gcggcggccg tgagcaacgc gcacttcccg gacatcggca tctccggcta
     7501 tgagggcctc ggcgattcgg gcgacagcag aacccggcag aacgtccact cgtttcaggc
     7561 aaacatgacg aggatcggat cgagccataa cttgaagtgg ggcttcgacc accggatgta
     7621 ccaggcgaat taccaggaca ccggcaatgc ctcgggcagc ttcagcttca atccgaactt
     7681 cacgcgggga cccgatccgg tttccaacac caacacggga cacgctgtgg cgtcgtttct
     7741 gatgggtacg ccatcgagcg gcagcataga caacaatgtg cagccggctt tcttcaacaa
     7801 atactacggc ctgtatctgc aggacgactt ccgcgtaaca cgatccctca ctctcaacct
     7861 tggcctccgg tacgaatacg aaacaccgag gatggagcgg cacgatcgca tggttcgcgg
     7921 ctttgcctac gatactccga gcccgatcgc cattccgggc cggcagctcc tggggggcct
     7981 tcaatttgcc ggggccggcg gggaacctcg atatcagtgg aatccgcagc gaacgaattt
     8041 cgcgccacgc ttcggcatcg cctggcagat cgatccgagg gtagttttcc gcgccggcta
     8101 cgggatcttc cgcgccggcg cgtccgattt cggaggtggt accgccgctt ctcccggatt
     8161 cagcgccacg acgccgatgg tcacgtccct cgatggggtt acgccggtgc accgtctctc
     8221 gaatccgttc ccggacggtc tgcttgcgcc gatcggctcg tccgaagggc tacgcaccct
     8281 taccgggcaa tccatagcgt tcgtggatgt gaacgggcgc gttccgtaca cgcagcagtt
     8341 cagtgccgga ttccaggtgc aaacaccggg caacttcgtg atcgacgcaa gttactccgg
     8401 cagccgcggg actggtttgt cgatcggcaa cgtcaacatc aaccaacttc cagtcgaaga
     8461 actggcacgc ggcggggctc tgcagcaatc ggttccgaac cctttctttg ggcaattcgc
     8521 ttcgggcgtg ctggcgtccg ccaacaccac gctcgggcaa ctgctgcggc cgtatccgca
     8581 tttcaactcc gtaacaaccc gcaatcccac cgtcggcaac tcgacgtatc atgcggcgct
     8641 actgaaggtg gaacgccgga tggcgaacgg cctgaccttc ctcgcgtcct atacggtgtc
     8701 caagctgatc gacgacgtgg gcgctccgca aaataactac aacatccgcg gagagcgggc
     8761 gctttccgtc ttcgaccggc cgcagcgcct ggtcgtctcc ggactatacg agcttccttt
     8821 tgggcccggc aaagcgctga ccggcggttc gagcccaata gttcacagga ttatcggagg
     8881 ctggcagttg aactggatct ccacgttcat gtccggcgcg cctctggccg tcacaagcca
     8941 ggtcaatacc actggatctc tcggcggcag ccagcgcccc gatagcaccg gagtgtcccc
     9001 ggccttggaa ggcccaaacc gcagccgatt ggatcgctac ttcgacacct cgcagttccg
     9061 cgccgccgct ccgttcacct ttggcaacct gtcgcgccag ctgcccgacg tgcgcgggcc
     9121 cgggcttcac aattgggata tctcgctcat caaaaacacg aacatcgcgg aaacggtgag
     9181 attgcagatc cggggagaag cattcaacgc tttcaatctg ccggcgttcg acaatccggc
     9241 cacgtcgttt ggcgccagca cgttcggccg catcagcgcc gtccagaacc gcgcaaaccc
     9301 ggctcgccag attatgctgg gcatgaagct actgtggtaa caaggacgga atgaagagaa
     9361 gcattttcgt attctgtgcg ctgctcggcg gttttgcggc cgggcagcac gacgctgcga
     9421 ttgagttcat ccacgtcacc gacacgcatg ttaccgattt gaagcaggtt tccccgccac
     9481 ttgtgaaagc gcgcgagcac tttagcgacg gcgagcgccg gcttgccgct ctccttgccg
     9541 gcaccggacg tccctccact gcatcgttcg ccctgattac gggtgacctg atcgatgcgt
     9601 tcagttacga gggccgaagt gccgatcggg tgttcggaca actcgacgca ttccgccgcg
     9661 ctaccgcgag gagctccatt ccgctgtatc tggccctcgg aaaccacgat atcgcgcact
     9721 acggcctcgc gccggacggc gtgaaaccca tggcagacca atccgtagcc gccgccgctc
     9781 gtgcagcctg gaccagatcg atggagtgct tccgcgacgg cacgtactac gagttctcca
     9841 aacagtcggg aagcacacgc tacgtctttc tgatgctgga caacgggtac actgccgcgg
     9901 gcgcggcggc cggacagcaa ggcgttcgta ttgcgcatga gcaactgtac tggctgcgcc
     9961 ggcgggcggc tgccaatcgc gacgccataa tcgtcctggc gatgcacatt ccgctgggga
    10021 aagacgggac gtccgaggcc attcgcagtg ctgtctcgga ggcgcccaat atcggtctga
    10081 tcctcgccgg acacaaccac agggaccagg tcgaggaact tgttttgggc gcttccacgg
    10141 ctgttcaggt tcgcaccgcc gcattgggat atggcgcgaa caactgccgc cgcatccgcc
    10201 tgcttccgga ccgcatcgag atctacaaaa cgggagccca cgaagagctg gaacgaacgg
    10261 tcgtggccgg cagaactgtg cggccggcag cgtagcgata ttcataggag cataaccccg
    10321 taaccgtatt ttaactgcct gttgacgcag tgtttgtttg gggctgttac aaaagaggtt
    10381 cgatcattct acacaggctt caagggagct actagaaaag gagattgcag atgaaacgtg
    10441 ccattttttt gatatcggct ttcgctgttg ctcaccccgc gtgggctcag gagtcccgag
    10501 gcagcattgc cggagtggta cgcgatcagc agggtgcttc tattgttgga gcgacggtgg
    10561 tggtgacgaa caacgaaacg aacgccgcaa accgcaccac cagcaatgcc agcgggtact
    10621 ttgaggcgag cttcctcaat ccgggaggat acacagtctc tgtcgaagca accgggttcc
    10681 gccgcctcgt gcgatcaggg ctgacgctga gcgtcggaag ccggctggac ctcgattttc
    10741 agcttgagat cgggcaggtc gccgaaacag tggaagtgac cgcggaagct ccgctgctcg
    10801 atacatcttc ggccggaggc ggacgtgtca tcgaccagca acaaatcctg caactgccca
    10861 ttgccgacat gaacccgttc acgctggcag ccctcgcgcc cggaatgcag tggacgggca
    10921 acccgggctt ccgccgtcca tcggacgtcg gaggcacctc ttcgttcaac accatgggcg
    10981 gagtcggcca gaacgagtac acgatcgatg gctcgccggt gacggggaca agcaggcgtg
    11041 tcggttttgt gccgccttcg gacgcggtga ccgagttcaa gctcgaggcc aacaacttcg
    11101 acgcttcgta tggacactcg acgggagcgg ccatcaacgt ggcgacgcgc tcgggaacga
    11161 acagatatca cggaacgttg tacgatcagc actggcaaca gcggtggaac gcgactccgc
    11221 acttcacccg cctgcagttc gaaaatgctg tgcgccgtgg cgaaaaaagt cccggcgatc
    11281 aaaggcaggg ctccggccgc tccaattttt tcggaggcaa tctcggtggc cccgtgcgta
    11341 ttccgaagct gtacgatggg acggacaagc tgtttttctt cttcagctac aacggccatt
    11401 acgaaaggac gtgggacgcc gactcgccga atcgcaccgt gccggcgcag gcgtggcgag
    11461 acggcgattt ctccgatttg ctggcgatcg atgcggttcg ctataccatc tacgacccac
    11521 gcagcgcgcg tcttgtcaac ggccgtgtgg tgcgcactcc cttccccggc aatcgcggcg
    11581 tgcccgtttt taatccgctg tacgactatt acacttccct ctacccgcaa ccgaacaacg
    11641 tacctggact cgtatcgccc gagggcttcg ataattactt cgccgaccgg actgtcaaca
    11701 cgctcaagta caacgcaatc gtgaaccggg tggactacaa catcaacgag cggaaccgcg
    11761 tgagcggaag gtggcaatac agccgccgca cgccgaacac acgcgactgg gcgtatgaga
    11821 cgaggcccgg tttgctggcg gttggatccc acagggtgaa caaaggagcg gcgggtaatt
    11881 acgtttgggt gctgaacaat aacaccctgc tcgacgtgag cgccagttgg gcccgcttcg
    11941 ccgatggcag ccggaacgat atgcggaatt ccatcaagcc gagcgacgtt gggcttccag
    12001 cgtatatgga cgagaaagcg ggcgactttt tcacgctgcc ggcgatccag ttctccgaac
    12061 tggaaaccct gtcgaccacc tacccgatgc tgaccgcctc cggcacgacc ggcgaattcc
    12121 ggctgggggt gactacattg aagggaggcc actcgttgaa gatgggttac accgagcgcc
    12181 gctattggtt tgcctccgcc gacccgggat attcgtctgg tatcttcacc ttcaacaatg
    12241 cgtatacgcg gcaggccgac gacaccaata cagcctcgca gcatggactc gagtgggcgg
    12301 cgtttcggat ggggctgccg tcgtccgtca gcattgacac ggtagactcc gcctactggt
    12361 ccacacgcgt gcgctcgttg tacgttcagg acgactggcg actgaccaac cgcatccgaa
    12421 tcaatctggg cctgcggtac gagcgcgagg gcggcatcac cgagcggttc aaccgggggc
    12481 tcagcggtga gtttcttcac gatgtcagac tgccgttcag tgagattgca gagagcgtct
    12541 atgcgcgcaa tccgttgccg gaactgccgg ccgcgcaatt ccaggttgcc ggcggcacct
    12601 cgtatctggg ccaattgaag gacacgttta cgaatggcac gcaccgcctg cttccgcgct
    12661 tcggattcgt cgcggagatc gccaactcga cagttttgcg cgccgggtac ggttggtatt
    12721 tcgatacgct caacgcgaac aacacgcaac ccagccagca cggttatagc ctgccgacgg
    12781 cgactgtgat cacaaacgac acagggctca gcttcaccgg cgtgggaccg gccgcgaacc
    12841 tttcaccggc gaacaacctg atgctggatc cgtttccggt tcgtgcggac ggaacgcgct
    12901 tcaatgttcc ctatggaaac cggctgggaa cctcggcgcg cgccggaacg agccatacct
    12961 tcttcgctcg ggattatgca cccgccgcgc agcaacgatg gaagctcggc gttcagcgcc
    13021 aactcatgag ggatctggtg gtggaaatct cgtacaacgg tgcattctcc cgcatcccgg
    13081 tgaaccagcc catcaatttc ctgccgcggg agtattgggc aacgggcaac gtacgcaatt
    13141 cagcgcggga tacagacctg aatgccaacg tgccgaatcc cttccgcgtc acgaatatgc
    13201 cgtcgcttgc gacctcggag ccgctgctct atcagtacct cagcaccgtt tccttcttta
    13261 cgagctccaa catccggaaa aatcagttgc tgcgtcctta tcctcatatg acgacgctca
    13321 atggcgtccg cccgggcggg tcgttcgaag atactcgtgg acgcaactgg tacaacgacc
    13381 tgcagcttat ggtcgaacgc cgcttcagcc ggggattcca gtcggcgctg atgtacacgt
    13441 acgcgagcag cgcggtgcag gacgtatacc tccacgaatt cgactcccag ccgtcatggc
    13501 agccgaataa cagcgtccgg ccgcaccgct tcgtgtggag cgcgatctgg gagcttcctt
    13561 tcgggagagg gcgcaaatgg gtccagaata acccgatcca gtatgtaatt ggcggatggc
    13621 aactgagctg ggtatatcag tatcaatccg gcccggcgac cacctggggc aaccggttct
    13681 tctatggcga catggcgaat ctgaaggacc tgctcaatgc ggacgaggcg cacggccgcg
    13741 acattcacct ctggttcgat ccgtcgattt cttatcgcgg aacaggcgtc atccccacga
    13801 acttccaggg ctttgaagga cgatccgccg ctcagccggg ccagtatcat gtgcgcgtgt
    13861 tcccgacgcg gctgagtatc ctgcgggccg atggaatccg gaactgggat gtgaagatcc
    13921 tccgcaagtt tcgggtcacc gaaggcctga gcgccagctt ctcgctggac atgttgaatg
    13981 cgaccaatca cacgaacttc agcgcgccga atacagaccc cacaaacacg aacttcggcc
    14041 gcgttaccag ccagaatggg ttgagccgcc gcctgcagtt gaatctgagg atcgagttct
    14101 gatgcgtctc accggtttcg ctctttggtt tcttgccgcc gttgccgcgg cgcaaacggc
    14161 cggcgaggtt ctgcggccat ggtcgcctgg gacgctcgac attcatcaca tcaatactgg
    14221 gaagggcgat tccgccctgc ttgtcttccc cgacggcaca tcgatgctgg tcgatgcggg
    14281 caacacgctg cgccccggtc cgcgcgtcac tccccagcgt cccggcaaca cgagaactcc
    14341 gggcgagtgg attgcccgat acgttcggca catgctggaa cccacaggta ttgtccggct
    14401 ggactatgct ctgctgaccc actttcacga ggatcatatg ggtgaactgt ctcaagctgc
    14461 ccggagatcc gccggaggat accaactcac cggtatcacg gaagttggcg atgcgattcc
    14521 aattgcgacc atgctcgatc gcggctggcc cgattacgcc ttccctgggc cggtcacggg
    14581 accgatgatg actaactatc gcgagttcct caaatggcaa gtgcagcatc ggaagatgcg
    14641 cgtggaacgc ttcgaggccg gccgtaacaa ccagatcggg gcagtgaggg agccaggccg
    14701 gtatcgcgat ttcgaggtgc gcaatatcgc cgtgaacggc gagatttgga ccggcgttgg
    14761 ctccgcgacc cgcgcgcact tccctccgat tgactccgtg ccaaaagaca atctgccagc
    14821 cgagaacatg tgcagcgcgg gcttgcgggt cagctacggc aagttcgatt acttcacggg
    14881 cggagacatg ccgggtctgc cggcggaggg aagccctcca tggcacgacg tcgagacgcc
    14941 ggtcgcaaaa gcggttggac ccgttgatgt gcatacactg aatcatcacg gcttccacga
    15001 ctgctgcaac gcttttttcc tcggcgcctt gcggccgcgg gttcacatta tgtcggtcta
    15061 tgcgccgagc cacccgggaa tgcgcgtgct ccgccgcctg caatccacgc gtatttaccc
    15121 cggccctcgc gacgttttcg ccacgaatct catgcaggaa acaaaggtgg tgattggcga
    15181 cgcgctgaat cgtctgaaga gtacgcaagg gcacgtgctc gtccgcgtag ctcccggcgg
    15241 cgcaagctat cgtgtcgtca tcctggacga cagcgccgag acgtatcgag ttacggcgat
    15301 tcacgggccg tatgaatcga gatgatcgta tgaggcctcc gctcgcattc ttccgttgac
    15361 aggagccgaa caatcctggc gcgtggtctt cccggactcc gccgcccgcc gctaccctcc
    15421 gctcacggca gcttttctac gcgaccggag taagtagata aaaaacggtc cgccgagcat
    15481 cgccgtaatc acgccaaccg gaatctcgga aggtgcgagc agggtgcgcg ccaccacgtc
    15541 gcagactgcc aggaacgccg cgcccagaaa gaacgcgcag ggaagcagaa tacgatgatc
    15601 cgctcccagg cgcaggcgca atgcgtgcgg gacaatgagc cccacaaaac cgatgggacc
    15661 ggtgagggcg gtgacggaac cggcgatcag ggatccggcg aggtatccgg tgagcagcaa
    15721 ccgggaggtg gagacgccgc gcgcttccgc ccactcctcg cccacggcca gtacgttcca
    15781 ctcacgcgct ttgaagaaca gcacgcagga tgcggccaca accaacgagc caatccacgc
    15841 cagcgtggag tagttgaccg cctcaatgcc gcccatcagc cagcggacaa tcgcaaacga
    15901 ctggccgaat gtggacaggt tttgcaggaa caggatgccc gccatcgaga tgctgttaac
    15961 cgtggtcccc gtcagcaaca gagtaaatga cgacatgcgc cgcccctcgg tcgcgacccc
    16021 cagtacgatg agaagcgttg taaacgcgcc cagcgcggct ccaaaccacg tagccggtac
    16081 tgcggcgatg gtcccgattc ccgccgagat ggtgataacc gcgcctaacg atgcgccagc
    16141 ggagatgccg agcgtaaaag gcgttgcgag tgcgtcgcga agcaccgcct gaaacagcac
    16201 accagccaca gcgagtccgc cgcccgcgat gagcgagagc agaacgcgcg gcacgcgggc
    16261 gtagaagaga atttcaaaat cgggcgattg cccggcgaag gcgcgagaat agctgagggg
    16321 cgcggagccg atcagcgggg cgataattcc cgccgcgacc gccacagcgg ccgaaagcgc
    16381 caccgagtag gcaagccgcc ttgccggcgc accgtcaact gccatagaca atccagggcc
    16441 gccccgcgcg cgtgtggctg atctcggcag ctacgccgaa cacatcggcg atgatcttcg
    16501 cgctgatcac acgctcggga ggcgcatcga ttacgatgcg gccacggttg agcatgacga
    16561 tgcggtccgc gtaagccgcc gcgaggttca aatcgtgagt caccgcgacg atcagcagtc
    16621 cccgcgtgcg gcacaactcg cggaggagtt cgtaaatggc aagctggtgg cggatgtcga
    16681 ggaacgtagt gggctcgtcg agcagcagcg tcctgggctc ctgcgcaagg gccgaggcca
    16741 gaatgacgcg ctgcctttcg ccgccgctca acgaccggaa atccctttca cgaaaggcga
    16801 gcgtgtccgt tatccgcatg gcgcgttcga ccgctttgaa gtcgtccggc gactcgaaga
    16861 gtccattctc gtgcgctgtc ctgcccatca ggaccacctc ttcggcagtg aacggaaatt
    16921 ccagccgcag gttctgcggg acgaacgaaa cttcccgcgc gaagaggcgc ctgcgccagc
    16981 gaaggacctc ggttccgccg tagaggcatc gtccctcata ctcgccgcgg agacccgcca
    17041 tgatgcccag catcgtggat ttgcccgctc cattcggacc tgcgatcgcg actagctccg
    17101 gcgccgtaaa accggcgtgg atgccctcca gcactggagt gccgttatag gtcttcccga
    17161 cgctctcaag taaaaagctg gggttcagaa tccagcctcc ggatggatca tggccgcgaa
    17221 ggcgcgcgcc gcttccacca ttcgcggccc gggaactacg aatatatccg aggcgatggc
    17281 gtagacgcgc ccattgcgga cggccgaaag caccggatac ttgcgccaca aggcgacaac
    17341 ggcacgtttg cgttcttccg ttacgcctgc cgggtcgctc atttctccca tgtcgatgat
    17401 gacttcgggg tcgcgcgaca acacctcttc gagagagacc cttggatagg ctccggtgac
    17461 ggattcgaaa atgttcttcc cgcccgcaat ctcgatgagc tcgtgatgat aggaaccgcc
    17521 gccggccgct accattcctt caatcgctcc gggagtgcgc ccggcgagga acatggtgcg
    17581 gggacgtggc agcttcgctg ttttgcgccg cacttcctcg agctgcgcgc ggatggcttt
    17641 ttccaccgca cgcgccgctt ccggcacccc ggccgcggca ccaatcgcat gcattgaagt
    17701 aaacacatcc gccatggtct gctggtccac ttccagtgtt ttgaggccca tattctggag
    17761 ccgccctgcg agcccggtag gattgcggtg aatgatgacg aggtcgggcc tcagccccaa
    17821 aatgacttcg agattgggat tgagaaagtt cccgatcttg ggcttacggg cggcttcagg
    17881 cggataccgg cagtatgctg tgaccgccac cactctcggg cccaacccca gagcgtaaag
    17941 agtctccgtg atgctgggag cggtggaaac gatgcgcgcg ggcggggcgg cccataaaac
    18001 ggcggcaacc agaaacgcaa taagagcacg cattaccatc cgagttggac tcctccacga
    18061 aacgtgcgcg acaggctgct gtaccctagc gcctcttcgt accggctgtt gagcagattg
    18121 tccaccctga gaaatggcgt cacgtgtgaa tggaggttcc acgaacctcc ggcgtagacg
    18181 ttctgatatc cggaattccg gctgacaccc aggaaatcgg tgtcctggcg ctcccctacg
    18241 agcgcacagc ctacaaagcc cgaccaccgc cgcggtgcga acgagatcga gagcgaaccg
    18301 gagttgcgag gtcttctcgc cagttcctga ccgatgccag tgaacagact ggagggatca
    18361 ttcgaatgcg tgatgcgcgt gtacagacgc gtgtaggctc cggaaacatg aagatcgctc
    18421 gccagccgcg cgcggcccga gaactccaat ccgcgggccc aactgcgctc gacgttgtcc
    18481 cagcttccga agaccggcgg cggaagcgat acgaaaacaa tcaggtcgtt aaaagaattg
    18541 tggaaggcgg ctacttccgt ccgcaggcgg cgcccgaacc attcctgcac gacacctgct
    18601 tcgtagctgt tcgtccgctc gggacgcaga tccggattcc ccacataaaa tgccgtccgc
    18661 gaaaagttct gaaccaggct gggttcggtg attccccgtc ctgcgctggc gcgcagaaag
    18721 gtggaagtga gcggcccatg ctcacccgct acctggacgc tgacggctcc ccgtggcgcg
    18781 actttcgtac caaagacatc gctgttctcg attcggatac ctcccgacag gaacacgcga
    18841 cggccgatgg tgtactgttc atgcagaaag acaccatggt tgttgcgtgc cacgtcgcgc
    18901 tccgagacgt tgccctgctg ccgctcatag tcataaccga aaacgaaaga cccgccggta
    18961 tgcgccaagt tgccctgata ctgaaattcc ttgcgtgaat ggagcgtcac gaaaggaggg
    19021 tcaaaggggt agagagtgac ggattgagtg atcaggcgcg tgccgggtgg ccgcgttgag
    19081 ggcaggttac gggcgtccaa tatcgcttcc cggtatacgc gaggcaccgg cgtccgcaca
    19141 tcgcgcacca gcaacgcaag ctcatagggg ccatccatcc cgaagtccgt gaaaacatct
    19201 ttctggcgat ggtacccgaa tccgaattgc tggacgaagc ggcttccccg cgcatcgtga
    19261 attcttgcgg acaccagcga atcgcgtgcg gtttcatggg catcgtggtc tacgagtccg
    19321 tagtggacct gtcccggaac ccccagtgcc gcatcaaagc tgcggtaggt tccgcgaaac
    19381 tcggtggcgg gtgaaaaccg gtagcccacg ttcgcgcttc ctgtggtgtt gcgatagtag
    19441 tcgttcgtga attctcctac ggaatggaac tgttcggcat tgagcgaata gtccaggcgc
    19501 tcacccgaac ctccgctcaa tcccgctacc cagcggtccg tctgaaatgt cccgcgttcg
    19561 taggaaaagg taccgtgcgg cacggttcgc tccggatctc cgcggcgtgt gaatagctga
    19621 atgactccgg ccgaagcttc ggcgccaaac agggcgctct ccggtccgcg caccacttca
    19681 atccgttcga gcgaactcga agtgagatgt ccgaaattca actcgccgcc cggatcgttc
    19741 atcggaacgc cgtcgattag gacaagcgtg ccggtccgct gcgcacctcg cgtgaaaact
    19801 gccgagagcg cgccatagcg gccgctccgg acgacgtgga ttccggggag ctcccgcaac
    19861 agatcggcca ccattgggga ttgttgtgtt ttaatctcgc cggcggtgat tacattcacc
    19921 gccacgccgg cggcttccgc tgtggtctgt gaccgcgtcg cggttactac gacgcgttcg
    19981 acgacaccgg ccagatcgag atcgacgcga ttccctgctc ccgccttaag atcaatctgc
    20041 tgttcctcga agccggatct ggttacggtg gccttgcatt cgtccggtcc ttcgatacga
    20101 aacgtgccgt caaagccgga agtcgctctc ctgtcctggc actcgatgac ggctccctgg
    20161 actctgcgcc cggtgggatc aaagaccgtt ccctgaatcg gagcctggct gaatagaggc
    20221 gcacaaaacg ctatgatgac gattcctgca aaagacaatt cgtgcatgaa acgttctccc
    20281 tcggaacgca tcactcatcc agcgaggaac cggtctcctg actttgcgac tcggctatcc
    20341 gaagccgggt gtatggcctt cccgcttaca cagtgaccac acagccgaag tttcctgtcg
    20401 cttacagttg cggggcagtg gcggattctc accgccttcc cgtaaaattt cctcgcccgc
    20461 tggtgaaggt cagattttta ccggagctgc ctgagcagcc cacagcaagt aagtataacg
    20521 cagcgggacg agttggttgc gcaatcgtaa ggtttactca atcccgtact cccgcagctt
    20581 gtcgtataga aggcgccggt tgatcttcag gatctccgcg gtcctggtcc ggtttccacc
    20641 tgcctgggcc aatgcgcgct cgatcaaccg ccgttcgagt tgggacacga gcgtgtgcat
    20701 gccctcttcc agggacacgc ttccgaaggg gtccgcgggg tcttccatcg tctccggcgc
    20761 cagatgctcg ggtgtaatcg gcgctccgcg gctgagcacg agggcccgtt ctaatgtatg
    20821 ttcgagttcc cggacatttc cgggccattc gcgctggcag agcgcctgca ccccctcagg
    20881 agtaattccg ctcagcggca gcttacgttg ggacgacagc ttcttgagca aacgccaggt
    20941 gagttccgga atgtcttcac gccgcttgga gagcggcggc agccaaatgc tgaacacgtt
    21001 gaggcggtag tagaggtcct gacgaaacga attcccgcgc acggcggctt ccaggtcgcg
    21061 attggttgcg gcgatgattc gcacgtccac ggtgaacgtc tgttcggagc cgagccgctc
    21121 caccgtgcgc tcctgcacca cacgcaacag tttggcctgg gtcgagggtg acaattcgcc
    21181 gatctcgtcg agaaaaaccg ttccgccatc ggccagctca aacctgccct tccgctgcga
    21241 cactgcgccg gtgaatgcgc cgcgctcata gccgaacaac tcggcttcga gtaacgtctc
    21301 cggaatagcg gcgcagttca cggtgatcag gcgccgctgc cgccgtgggc tgtgacggtg
    21361 gagcgcccgc gcaaccagtt ctttgccggt gccggattcc ccccggatga gaacagtgga
    21421 atcggtaggc gcaacctggc cgatcaattt atatacctgc tgcatggccg ggctgttgcc
    21481 tacaatctcg gcctcgttct gttcctcttc ggcaacctcg ccgtaacgcc tgagcgccgc
    21541 cgtctggcgc cgtgccgcga tcgcgcgttc gagttggatc agtacttcct caaaatggag
    21601 cggctttgtg aggtagtcat aagcgcccag cttcatcgcc tcaatcgcga cggcggacgt
    21661 cccgtaagcc gtcatcacaa ggacagccgg agcgcggccc caatcgcgcc attcacgcaa
    21721 cagcgacagc ccgtcttttc cgggcatgcg gacatccagc agaacggcgt caaagtcccc
    21781 ggccgccacg cattcactcg cggctatgcc atcgacggcg cattcgacgt catagccctc
    21841 ttcttcgagc agtgccacca aagcatcgcg cgcacgcgca tcgtcctcgg ctaccagcac
    21901 ccggccgccg ctcacaaggg ggcctctctc gaaggcacag ccagggtgaa taccgtgccc
    21961 tcgggaccgg tgtcaaattc cagagtgccg ccgtgccgca gaacgacctc gcgcgacatc
    22021 gccaacccga gccctgtgcc tgcatccttc gtggtgaagt aagggtcgaa gatgtgatcc
    22081 ctcgcatcgg gcggaatgcc cggtccacag tcctccacgc ggatacgcaa attatctccg
    22141 tcgcagccgg cgctgattcg cacctcctcg cccgagggag acgcgtcaat cgcgttgagc
    22201 agaagattga tcaacacctg ctccagttgc agtccatcca cttcggcttc gagcggggcg
    22261 cccgcttccg tgtcgagccg cacttggatc tgtttcgatt gcgcctgctc gtgcacaagc
    22321 ttgatggcgc gatcgagcag cgggcgcaca ttctgccgcc ggcgctcttc caatccggtt
    22381 ttcccgaatt ccaacagtct acccaggatc tgctccaggc ggtcgacttc ctcgatggct
    22441 tcccgcgctt cgtcaccacg caaggcgccg cgcctcgccc ggcgttccag caactccagt
    22501 gtcagccgga tggagttgag cgggttccga atttcatgcg cgacccccga cacgacccgt
    22561 cccaaagccg ccaatctgtc ctgccggcgc agcctggttt cgagagcgcc gcgccggtcc
    22621 gacatgcgat taatggcctg agcgatcgcg ccgaattgtc caccgatcgc aggcatccgg
    22681 taagcgaaat cctcctccag acgccggagc ccttcgttta ccgagccgac gccccgccgc
    22741 aggtagagca tgatggagat aatgccgccg agaccgagca ccgtcgcaaa tacaagcgcc
    22801 gccagccacc atcgccggcg ctccagcacc ggatcacgcg cttccgccag ccgcttcagc
    22861 gtccaggcga ccgcgccgga atccgcgcga tgtgccgcag ccacgtacac gtcgcgcccc
    22921 aggctggcaa cttcgaccac cgtctcaccc ggatcgccaa tccggacgat ggcgctatgc
    22981 gctacatcgc gttcgaactt cgccagcgtc tgctgcgatt ccagtccacc agggggcaca
    23041 tcgcccgcca tccggcgctg ctcggccagg tagaagccgc cggccattcc ttcgtaggcc
    23101 cggagcacgg cgagactgag tccccgcaag gaaacatcca acgcctcaaa tggcaaaact
    23161 tccaccggct cattgctgag attggcgcgg tcccggtact gctggctcaa ctcctctgcc
    23221 gcgcggatgc actgctcacg cgcttccgcc accagccttt cacgggtgct tgtcagaata
    23281 tcttcggcca gcagccaggc caaagcgagc atggaaaggg aggcaaagat agtgaccgca
    23341 atctgcgccc gaaaactatc cagagagatt cgcactcgcg ctccggttcg aaaagaccgc
    23401 ttgctggcgc gcgcggctcc gtttcagagc cgcgaccgcg agggagcggt tttttaccac
    23461 gggttgttac taatctcaat ctaacggagg cggcatcgga atgggaacgg ccgcttgcgc
    23521 gagcttctcc aggccttcaa tcacttcgtg cgaagcctcc gccaaccggc gtgccagctc
    23581 gaacgtgccg cgatcgtaag cggatctggc ctgctggtac agccgtcttc ccagccgtac
    23641 gtattcgggt ccgaaaggat ctttgctttg ggcggaaaag tactcgccct gtttgacgcg
    23701 gaaataggtc cgctcgagca cgcgagccgt cctccggcgt gcatccccat cagcgctttc
    23761 cgaacggctg ctccgggatt gcttgcgtgc ccccttcaat tcttcgctgg cgtcgaggaa
    23821 agagtcgatg gcggagcgga tcctcgaatc cgtgtaagtt cccgcgtcga ggggcttcca
    23881 tttggccatc agctccgtca cccgctcatg tagaaagcgc tgttcggagg tgacgccgcc
    23941 gtcggattcc agacgcttca ggcgctcgga aaactccgcc acctgacgtc tctttttctt
    24001 cttgctggag tgctcatcgc cggcctccac attgtgcaat aggcacgctg cgacaaccaa
    24061 ccataaaaag attcccgtac gcatcaaaat gtaaatcgaa cggaagcttg caaccgccgt
    24121 gccgcactgg ccgctaccgg ttccccgaaa aacggcgagc tttggttccc gaccagccgc
    24181 gtgtagttga cacggttcag cacgttaaac ccgtcaagcg acaacaccac ggcgggaggc
    24241 cgctcgctcg cgccgcccga aagccggaat gcccgcgaaa gccgcaagtc cagtctggat
    24301 tcgccgggca tctgcaaagc gttcctgccc acgccattgg gccgctccag agtcaggccg
    24361 tcgcggttca gatccaggcc cgtggtccat tcatagggcc gcccgctcga cgccgaataa
    24421 atgatgccaa ggccgaagct ccgcggtaaa tcgaccagcc cggccgctcg aaagcgatga
    24481 cgccggtccc agtcggcacg cgcccactgg ctgcgcaggt ccaggctgtc cggcggaagc
    24541 ccgcccgtat cgttatggga cgatcccagg cggtatcgaa cctgtcctcg aaagaaccgc
    24601 agcatccgcc cgttcgccga aatgtgcagt ccctgcgatt tcatggttcc tgacgattgt
    24661 atctgacgga taaaaccgta ggagtccatc ggccgctcga aatccggcgg aagtggagcg
    24721 ttggcgtcga tggtgcgcca caggctcgtt ccccgcgtca cggaataatt ggcgctgacc
    24781 tggaaatccc gggtgaaccg gtgctcgatt cccccactga aatcgaagct gtaaggcagc
    24841 ttcgctcgcc gggaaagctc gacgatgttg ggcggcagtg cggccgtgcc gtcgatatcg
    24901 aggatcggat aagccggcgc cagaatctga agatcccgaa gacggagccc gttcaattgg
    24961 agcagatccg acacggtgcc cattccgagg cggtcgtaga acatgccgcc acccgcgcgg
    25021 atgacggtct ttccgctctc gttcaaaccc cacgcgatgg agagacgggg tgcaatgttg
    25081 ttgtagtcgc ccaagtgccc ctgtgcgtca tagcgtaagc cgagccccag ggtcagccgg
    25141 gacgacaact tgatatcgtc ctggaggaac gccgagccct gccagtaaaa gaaactgaca
    25201 tccggctgtc ccactcgaac cgtgtaggac agcgggcggc cggtcacata atcgtccagc
    25261 gaggcgaact ggtaggtacc gccgaaatta ttgcggtcca ccaggcgctg ccattcgagc
    25321 gtgggcgtca tgatgcccca acgcaacgag tgccggcccc gcgtgttcga aaacacccag
    25381 ttcagcgaca ggcgattgcg gtcctgagcc tgatcctgct gcgcgcctcc tccggtgaac
    25441 gcatcgttga cgacaattct tgcggcatcg ggcgttgcgc tcgtattggt ttcgtggctg
    25501 tttctgtagc gtatttccag gtcggagaag aacgtggcag acggaaacca gcggtatccg
    25561 aatcccgcga cgtgatcgcg gtcccgattt tcataagcgg attccgggag attgaagccg
    25621 ccgattccct gcttctgcca ttcgcgatcg tacccatatc tccacgacaa aacatgccgc
    25681 tcgcccacat agcggttcac gcggccgtag atgcccgttt cccgttcagg ctgcacgagg
    25741 ctctgctgaa gcaaccccga aggtgtgcgg gcaaacacag gctgctggag ctcgtcggtt
    25801 tgccgctcca cgctcaacag gaaggtagta ttgccggaac cggtgagcgg gccggtgaga
    25861 tgtccttcaa agaaccgccg ctgttgtgga ggcctggtgg gtgaaaaagc gttgcgggcg
    25921 ttcatgcgcg catcgcgcaa ccccgccatg aagctgccgt gaaactggtc cgagccctgt
    25981 ttggtgatta cttcaatacg cccccgtccg ggacgcgaaa actccgccga ataacgattc
    26041 cggttgatgc gcacctcggc gatggccgac ggactaacgc ctagctggtc ggtttccatg
    26101 ccatccacca ccagcgatac gccttccgac cccatcgcgc tatcgtccac gaactcgccc
    26161 gcggcggcca gaatatcctg gtcgaggacc ggaagcccgt acagcatttc gctatcaagc
    26221 tccagggcgt cggcgttccc ggtcggcgag agttccggtt cgtcgctttg cgcagttacc
    26281 gtcacctctt ccgaacggcc ggccaactcc agacggattt gcaggtttga tatgtctctg
    26341 gtgatctcga tttcctgttg cgagtccgaa aaacccggag agcgggcctc caggcgggct
    26401 tctccggtgg gaaggttctc gaaagaaaac ttcccttcca cattcgtttc ggtgctgcgg
    26461 gctccgggcg tttctcctcc tgtgtgcaag gttattttga ctcccggcac cggtaaaccg
    26521 gttgagtccg ttaccgagcc gctcacgcga tgcccttgta tccttgtgct cacgctctgg
    26581 gcctgtacgc tccatccggc cagcaacaat acgatcagat acctcattcc ccaagcgata
    26641 gtgcaagtgc tattccgacc cgatgacaag cgttttgagc aggttagcgt ccccatcgcc
    26701 aggcctcgcg tgcggattcg ttacggacag cgtgcggatt tgtatcaact acgacctgct
    26761 gcgcttatga tggataccta tgagaacact gctcgcaatc gccggcgtca tgacctcttt
    26821 tgcggctgcc ggccaggaag tggagaatgc tgaaaaggcc tgggccgccg ccgtcgcagc
    26881 gaacgactat gccgctatcg accggctgac gggagacgac ctgatttatg gtcattcgac
    26941 gggcctggtg gaaaccaaga gccagtatgt ggcggcattg cgctccggac agcagaaata
    27001 cgaccagatc cgccatgaga gcatgaacgt gaaaactcac ggcaacacgg ccgtggtttc
    27061 atcccgggtg cgcatgaccg gcagcacaaa agggaaaccg ttcgacaacc aactcatcat
    27121 gatgcacgtg tgggtgaaga acggaggcgc ctggaaactg gtgggccatc agacagcacg
    27181 tatgccgtga ggagcacggc aacggtgtgg aatgaagact ctcgctcaat tccggcaaca
    27241 ggcggttttc ttgagagaat cctccggctc taccaatatc gaaaaaacgc ttttgaaagc
    27301 tttgaaggtt ctgaagctgt ttaatatccc gcactatgtt tgcggcggct ttgcagtgca
    27361 ggaacgtggt tacccacggt tcacaacgga cgtcggtatt atcgtgcctg atgtgtcgtt
    27421 cgccatcgag aagctgtcga taaacggctt caagcaaaac ctcggctcca acatgactgt
    27481 caccgaccgc gagacgaagg tagaggttga ccttttgccg gggggccaaa agattgatcc
    27541 gggaccgctc acgttgccga tacccaccca cgtttcagac aaaccgcaaa ttctgactct
    27601 ggaaaagctg atatcagcca agctatcaac ttacatgggc cggggaatcg aacgcagtca
    27661 agactatgcc gatgtcgtca agctcgttca agcaaaccac ctgccgagag acttcggcgt
    27721 tgaccctaga gtacgggacg aataccacaa gatttgggac ggcctgcacc agaaggatat
    27781 ttaagctcgt ttacagccgt cgcatcggct gccagactta cttgacgccg aacgccgctt
    27841 cgttcagcgc gtcgatgatc aggcgggaat cgaggccgtg ttttcgcgcc actacttcga
    27901 tggagcaatc gtcgcaggaa tcgcggaacc tgaggttctc gaaaacttcg acggtctgcg
    27961 gatactgcac cttcacgtcg cgaatcacca tgctggggcg tatgagctga tcaaaacgga
    28021 ttgccacgaa ttaccctcca gtttaagcct cagtgaatac agggtaccag aaactctcga
    28081 tcccggccca gtggttgaac tttcgcaccc cggcatctct cttgagtgat cctctggggg
    28141 aaataccccg tcttctccct aagataatga tttaaaacta attcagtagc ggtacaaaac
    28201 gtgcaccact gttatgtggg tttagtctgt gtggagagat ctgtataggt tcaagctgac
    28261 ctcagccgat gaagcgatcc ggatcgtgga atcagggaat cgcgtacacg tgcaccaggg
    28321 gtgttccgaa ccggaggaac tggtggcggc tctgatgcgc cgcgcaccgg agttgacgga
    28381 cgtggagatc gtccactgcg ccacgatggg aaccgcggac tatacgcggc cggagtttga
    28441 gggccacttc cggcacgcgg ctttcttcat tggcggcaat gtccgtgagg cggtgcaatg
    28501 ttcgagagcc gattacattc ccatcttcct gtcggagatt gaagcgctat tcgagagcgg
    28561 cgcgatgccg atcgatgtct ctcttcttca gtgcactcca cccgacgatt acggatacat
    28621 gagtttgggt gccggagtgg atatcgcact aaccgccgcc aggtattcca aacacgtgat
    28681 tgtcgaagtc aacgaccaat gcccccgcac tttcggcgat tgcttcatcc acgtgcgcga
    28741 cgtggatgcc atggtcgaat gttcgcatcc gttgcccgaa tatcgatcgc acgaagtgac
    28801 cccggcagat gaaaagatcg ccgcacagat tgctaacctg atacccgatg gagcgacgat
    28861 tcagaccggc atcggagcag ttcccgactg cgtgct
//
LOCUS       DASTMK010000356        13571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_177460, whole genome shotgun sequence.
ACCESSION   DASTMK010000356 DASTMK010000000
VERSION     DASTMK010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13571
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177460"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFQ79_15665"
     CDS             complement(<1..1015)
                     /locus_tag="VFQ79_15665"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0141160.1"
                     /translation="MSWPAKTQQDPFTALLICPNRALAAQFLETAARDGFQVLSQLEN
                     YPSRQTLDIRLRQIRPDLVVLDVGSNRGTAADLIRFAVTTSHQTHVVAIHSASEPELV
                     LSALRSGATEFLTAPFEIQVQQEALARLRRLRQPQTDNRPEPGHVLAFASAKPGSGAS
                     TLAAQLAFSLQALTKERVLLADFDVMGGTIGFCLKIAPEYSLSDLLATGDSTPAALWQ
                     GVVAAHAGLDLLPSPAVPPDEPPDTSRLHEIIESARSAYDWVLIDLPAIFHRISLITF
                     SQSDQAFLVSSAELPSLHLARKAAMLLAQLGIEKQRFQLVLNRATTRHGIRGAELEKI
                     LSCP"
     gene            complement(1038..2402)
                     /locus_tag="VFQ79_15670"
     CDS             complement(1038..2402)
                     /locus_tag="VFQ79_15670"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEU0141161.1"
                     /translation="MIRVWSAAISFVLLGASATASAQSAPEELKIPVGRSMVIDYPAD
                     VRQISTSNPDVVDAVAVTTREILLHGKGQGSGTVVIWAKTGQRTFYNITVDQNLEPLR
                     EMLRDTFPGESIKVQSGKDSLALTGHVSSKEVSDRAAALAAGFSKAVVNNLEIAAVPV
                     EKQVLLRVRFAELNRTAASSLGVNLVSTGAGNTPGQISTGQFASASASTLKGAIPGSA
                     AGTVSEFDISDVLNVFAFRPDLNISAFIKALQTRGLLQILAEPNLVTVNNKEASFLVG
                     GEFPVPVVQGGASMGTVTVQFREFGIRLTFLPSITGNGTIKMHVKPEVSTIDIANSVT
                     LSGFTIPALATRRMETNIELAEGQSFVIGGLIDDRVTESASKIPGLSNIPILGALFKS
                     RQENKSKSELVVMVTPEIVHPLDAKDPKPMPVMPREFMGPATAGDGTTLTILPPDRQK
                     PERK"
     gene            complement(2399..3277)
                     /gene="cpaB"
                     /locus_tag="VFQ79_15675"
     CDS             complement(2399..3277)
                     /gene="cpaB"
                     /locus_tag="VFQ79_15675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEU0141162.1"
                     /translation="MDRRFLTVLALSLVFALVVSGIFYQMTTRAATGKPANQATRDIV
                     VSKGALGVGVSIKPDHVKIAKIPADQFPKGGFSRVDEVVDRAVMSNILPEEPLLEGRL
                     AARGSGLGLAPIIPVGMRAVSVRVNDVVGVAGFVLPGMRVDVLVTGRPPSSDDTITTT
                     VLQNILVLSAGQTIQADTTGQAINAPNVTLLVSPEQAEILTLAGNEGRIQLVLRNGGD
                     QDVEITPGRTISQLYGKRGSRASEDGPSRPHVPKPPAPPAPRVVENVPPPPDEVIVIR
                     GTARTVEVVNPRGKEK"
     gene            complement(3313..3768)
                     /locus_tag="VFQ79_15680"
     CDS             complement(3313..3768)
                     /locus_tag="VFQ79_15680"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU0141163.1"
                     /translation="MTIALAIVLLLGVAASAEDFRRRQIPNWIPVAAVVSGLLLHTWT
                     GSWWGLLNAAGGALGGFAALLLFYLLGGMGGGDVKLMAGFGAVAGAGSLILALVLVFL
                     AGAGFALVVLALRALKRALSGRHRTDAEDASIPYAPAIFVGTLMAILGH"
     gene            complement(3765..3992)
                     /locus_tag="VFQ79_15685"
     CDS             complement(3765..3992)
                     /locus_tag="VFQ79_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141164.1"
                     /translation="MRQSKPRGRGNRILEGGLGAPVETTGWDDTGWTLPSGQRITDFA
                     LEHAFLAPALMGPLTAGEELAVGSGAESGPK"
     gene            complement(4188..5498)
                     /locus_tag="VFQ79_15690"
     CDS             complement(4188..5498)
                     /locus_tag="VFQ79_15690"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141165.1"
                     /translation="MELSRRNFMQAGAALAAAQGNRSVSANDRIQVGVIGCGARSHEL
                     MHALMQLPQAEIVAVVDAYKGRVERALDRVGGKAKSFKDYREVLAQPSIDAVLVVTPD
                     HWHKQMVLDAIKAQKDVYCEKPLTYRSTEGVEIIEAAQSAKRIVQVGSQGMSSPIQKK
                     AREIIQAGKIGKVTLIRAAYNRNTASGAWIYPIPPDASAQTVDWEMFLGPAPKRSFSL
                     ERFFRWRCYDDYSGGIATDLFVHLCTTIHYLMGAKVPSKVMALGQLYRWKESREVPDT
                     LNAVLEYPEGFAVNLSSTFNNQTSAESSFEFLGTEGSLNLGWGGATLYPEVAVEDNRW
                     IVESWPKRLEEAYYRDPKVRQAEMPSTWKPDVLEGNQQLRQQGPDATVLHFSHWIDSI
                     RTRKPYWEDAAAGHHAAACAHMVNRSAKENKVVEWDFARDDIKA"
     gene            complement(5519..6925)
                     /locus_tag="VFQ79_15695"
     CDS             complement(5519..6925)
                     /locus_tag="VFQ79_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0141166.1"
                     /translation="MTILEAAAAIRAGDLSPVELTSRCLAGIRELNPRLNACLTVLEE
                     QALRRAKQAEEELVSGKDLGLLHGIPIVLKDNFYTKGVRTTCGSKLFANYVPAHDATV
                     TERLRDAGTVLLAKTGLHELAYGITSGNPHFGPVRNPWDPERIPGGSSGGSGAAVASG
                     MVFLGMGSDTGGSIRIPASYCGTVGLKPTFGRVSRHGVFPLGYNLDHMGPMTRSVRDA
                     AVVLQAIAGHDVRDKTSSRAPVADYLSEANRSIAGLRIGLPENHYFERVAPECAAAVE
                     SMSKVAEQLGASLVRLPFPDVQALNTVGRVILLAEASAILESRMAERDQFGPDVLALL
                     DQGRLLPATDYINAQRLRSVLCAEFSALWSRIDCFFTPATPMAAPRIGQSKVMFNGEE
                     EDVRLATTRLVRGINVLGYPALSMPCGFTESGLPLGLQIVGRPFEEGVILRVGTALEG
                     ATEFHTRKPPLYNSAIAR"
     gene            complement(6922..7095)
                     /locus_tag="VFQ79_15700"
     CDS             complement(6922..7095)
                     /locus_tag="VFQ79_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141167.1"
                     /translation="MKNWKLIAEGLDLGIPAPELEKICPALERLEKDCAVLRGLAPGT
                     EPAITFECGPDTQ"
     gene            7094..8350
                     /locus_tag="VFQ79_15705"
     CDS             7094..8350
                     /locus_tag="VFQ79_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0141168.1"
                     /translation="MKIHSTVFRGPAPLNRSGRDDIIQEAMSTRIEQMEPSPLTRLSE
                     EEEGFRAEVRRFAREQIAPYVREMDEKGAFRKEIIQQFFDLGLMGIEIPEELGGQGGT
                     FFQAVLAVEELSAVDPSAGVIADVQNTLVNNALMRWANDEQKRAYLPRLAGNAVGSYA
                     LSEAGAGSDAFALAARAEEKDEEYFLTGRKLWITNAAEAELYLVFATLDPGAGYRGIT
                     CFLVERGFPGFEVGKKEDKLGIRASSTCELILDACRVPKSNVIGEPGKGYKIAIETLN
                     EGRIGIGAQMVGLARGALDHALAYAKQRKQFGTAIAAFQGVQFALADMATELEAARLL
                     VYNAARLRDAGAPFVREAAMAKLYASEAAERIASAAVEVLGGVGFTKDYPVEKLYRDA
                     KIGRIYEGTSNMQRLTIAKQIFGKPV"
     gene            8383..9171
                     /locus_tag="VFQ79_15710"
     CDS             8383..9171
                     /locus_tag="VFQ79_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141169.1"
                     /translation="MRQLLIAATLCVFLTGTGMAAKADLSQAEVDEIITKFAAKEGEF
                     AKARENYTYRQTVRIQELDPAGNVQGRHEMISDIIFSADGKRTEKVVRAPVSTLKNIL
                     LTPEDEQDLRNVQPFVLTTKDIPDYHIRFLGKETLDEIPCYAFAVKPKQMEAGKRYFQ
                     GIVWVDDRDLQIVKTYGRGVGLLKKNSDNQFPKFETFREQIDGKYWFPTYTAANDTLH
                     FQAGPQRIRMIVRYEDYKQFKADTTIKFGEEVDTPPSVEEPKKP"
     gene            9277..10293
                     /locus_tag="VFQ79_15715"
     CDS             9277..10293
                     /locus_tag="VFQ79_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0141170.1"
                     /translation="MTFRRRVRAISRRARELRMIAKGAFSTDHPILAHLIPIRRCNLS
                     CKYCNEYDNVSKPVPTEIMLGRIDRLAALGTTIITISGGEPLLHPDLDQIITRIRRRG
                     ILAGLITNGYLLTAERIRRLNKAGLDHLQISIDNVQPDEVSKKSLKVLDKKLQLLAGH
                     ALFHVNINSVVGGGIRNPEDALVVGKRAVELGFTSTIGIIHDGDGQLRPLGGREREVY
                     HQMKRLEKSSYAQVNYFQDAIAEGRPNRWRCRAGGRYLYICEDGLVHYCSQQRGYPAK
                     PLMEYTVEDIRREYLTEKGCAPNCTVSCVHQISYFDFWRGEQTIRSADPAPEAAPLVH
                     IEMN"
     gene            complement(10312..12636)
                     /locus_tag="VFQ79_15720"
     CDS             complement(10312..12636)
                     /locus_tag="VFQ79_15720"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU0141171.1"
                     /translation="MTLGKTVFREITSSAILGIVLFTFVLFLQRVGRLFELLVRSSMP
                     IDTIGLLFILVLPFVLTFTIPVGVLVGTLIGLSRMASDGEITAMRAAGVPGRRVFWPV
                     MAFAALATAAAAATSLWLTPLSIQETYRVLNKLVAEQMTAEIRPRVFEETLTNTILYV
                     GDVEPGPVTRWKKVFMADLTPAGERISRTEEASDDPRITVAAEAIAVADVERSRIQVS
                     MLNGRTYEVGKDPAQYFNTGFPKGDQVLVAEPPEIRTAKAYSEMPTFPLMAEAEKSVD
                     ARIELHQRFALPLGCILLALVGLPLGVSTRKAGKSTAIVMTVLLAFTYYMALISLIGV
                     ARQGTLPAGIAVWMPNIVFAITGIILLARLERPGDRDWVGTAWTWIGRILRGLLHRLP
                     SRPGPLSRRSGGRMPLLPQIVDTYILNGFLFYFGLLLASFVLMTHVYQFFELLGDVIR
                     NNIPMQRVFAFLFFLTPKLLYDLAPLSVLVAVLVTFGILTKNNEVAAMKACGVSLHRL
                     AIPILLMSAGLSGALFAFDHYVIPDTNRKQDAIRNEIRGKPIQTYLRPDRKWIFGNGS
                     RIYYYRYFDPVSSVMGGISVYELDPGTFRLTRHISAERALWQPSLKTWIFQNGWMREL
                     GGGTDGETFRDFRGQTATFPELNEPPSYFLKEVKQDKQMNFHELAGYIRELQQSGFDT
                     VRLQVQFYKKFSVPLFVLIMALIAIPFAFVTGNRGAMAGVGVSLGIAITYLATNSLFE
                     QMGNVAQLPPQVAAWSPDAVFSLAGLYMMARMKS"
     gene            complement(12640..>13571)
                     /locus_tag="VFQ79_15725"
     CDS             complement(12640..>13571)
                     /locus_tag="VFQ79_15725"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU0141172.1"
                     /translation="SYIGPVLLEGEAAPQMFAQVLGDNLAMIRRPVSEPNRPIPLGQS
                     EIEGRIGSRVLPEWMDVVDDPTQSQWRGRPLLGYYPVDMEGVPPVPLTIIEKGTLKNI
                     LLTRQPVRGYSGSNGRARLPGNFGAKGATFGNLFIKASQTTSAEELKRKLIEMCRERN
                     KPYGLLVRKLDYPSTAPLDEIRRMAAGMAQSSGGRNPVSLPVLVYRVYADGREELVRG
                     MRFRNLSVRSLRDIVAVSGENHVFDFLDNTAPFAATGGATFVAPATVVAPSVLFDELE
                     LERSQEQLPKLPLVPPPSLSEGATASETARAHL"
BASE COUNT         2848 a         4003 c         3998 g         2722 t
ORIGIN      
        1 ccggacagct aagaattttc tccagttccg ctccacggat tccgtgcctt gtcgtggcgc
       61 ggttcagaac gagttgaaat cgctgtttct cgatgccgag ctgggcgagg agcatcgccg
      121 ccttccgtgc caggtgcaaa ctgggaagtt ccgcgctcga aaccagaaac gcctggtccg
      181 actgcgagaa cgtgatgagg ctgatgcgat ggaaaatagc cggtaaatca atgagtaccc
      241 agtcataggc ggaccgggcc gattcgatga tctcgtgcag ccgggaagta tcgggaggct
      301 catcgggcgg aacggcgggc gacggcagca ggtcgagacc ggcgtgtgcc gccacgacgc
      361 cttgccagag cgcagcgggc gttgaatccc ccgttgcgag cagatcggaa agcgagtatt
      421 cgggagcgat cttcaaacaa aaaccgatag ttccgcccat cacgtcaaaa tcggccaaga
      481 ggacgcgctc tttggtgaga gcctgaagcg aaaaggccaa ctgtgccgcc agcgttgacg
      541 cgccggatcc gggtttcgca ctggcaaatg ccagcacgtg ccccggctca ggtctgttat
      601 cggtctgcgg ctggcgcagg cgccgcaaac gggctagcgc ttcctgctgc acttgaatct
      661 cgaaaggcgc ggtgagaaac tccgttgcac cggaccgcag cgcacttaaa acaagttccg
      721 gttcactcgc cgagtgaata gcgacaacat gcgtctggtg agacgtcgtg acggcaaacc
      781 ggatcaggtc cgctgctgtg ccgcgattcg agcccacatc cagcacgacg aggtcgggcc
      841 ggatctggcg cagccggatg tccagggtct gccgggaagg ataattctcg agttggctga
      901 gaacctggaa tccatctcgg gcggcggttt ccaaaaactg cgccgcaagc gctctgtttg
      961 ggcaaattaa cagggctgtg aacggatcct gttgggtttt tgccggccag ctcatgcctg
     1021 cctctcgaat tgaacactca cttccgctcc ggcttctgcc ggtctggagg gaggattgtc
     1081 aacgttgttc cgtcgccggc cgttgccggt cccataaact cccgcggcat gaccggcatc
     1141 ggcttcggat ctttcgcatc caatggatgt acgatttccg gtgtcaccat taccactaat
     1201 tcgctcttgg acttattctc ctgacggctc ttgaagagcg ctccgagaat agggatgttg
     1261 gacaggcccg gtattttcga tgcgctctct gtcacccggt cgtcgatcag gccgccaatc
     1321 acaaagctct gcccctccgc caactcgatg ttcgtttcca tgcgccgggt agccagggct
     1381 ggtattgtga atccgctcaa ggtgacggag ttggcgatat cgatggtcga cacttccggc
     1441 ttcacgtgca tcttgatggt gccgttcccg gtgatagaag gcaggaacgt gaggcgaatg
     1501 ccgaattccc ggaactgcac ggtaaccgtt cccatactcg cgcccccctg cacaaccggc
     1561 acaggaaact cgccgccgac caggaagctc gcctctttgt tgttcactgt tacgaggttc
     1621 ggttccgcca gaatttgaag caggccccga gtctgcaaag cttttatgaa cgctgagata
     1681 ttcaggtcgg gacggaatgc aaacacgttc aaaacatcgc tgatatcgaa ttcggaaacg
     1741 gtgcccgcgg ccgaaccggg tatggcgcct ttcagcgtgc tggcggatgc ggacgcaaac
     1801 tggcctgtcg aaatctggcc cggtgtattc ccggcgccgg tcgaaacgag attgaccccg
     1861 agcgacgagg cggcggtgcg attgagctcc gcaaaccgta ccctgagcag gacctgtttt
     1921 tcaacgggca cagcagcaat ttcgagatta ttgacgaccg cttttgaaaa gccagcggcc
     1981 agtgcggcag cacgatccga aacctctttg ctgctcacat ggcccgtcag cgcgagtgaa
     2041 tcctttcccg attgcacctt tatgctttca cccgggaaag tgtcccgcag catttcacgc
     2101 aggggctcca gattctgatc gaccgtgatg ttatagaacg tacgttgccc ggtcttggcc
     2161 cagatgacga cggtgccgga gccttgtccc ttcccgtgca gaagaatctc gcgggttgtc
     2221 acggcgacgg catccactac atcgggattg ctggtggata tttgccgcac atcggcggga
     2281 taatcgatca ccatactccg gccaaccgga atcttgagtt cctccggcgc ggactgcgcg
     2341 gacgccgtgg cggaggcgcc caaaagcaca aacgaaattg cagcactcca gacccggatc
     2401 atttttcttt tcctcgcgga ttcactactt ccaccgttct tgccgtccca cggatgacaa
     2461 tcacctcatc aggaggcggc ggcacatttt ccaccacccg aggggcagga ggtgccgggg
     2521 gcttcggcac gtgtggacgc gacgggccgt cttcggaagc tctgcttccc cttttcccgt
     2581 acaactgaga aatcgtgcgg cctggtgtga tttcgacgtc ctggtcaccg ccattgcgga
     2641 gaacgagttg tatgcgacct tcgttgcctg ccagcgtaag gatctccgcc tgctcgggac
     2701 tcaccagcag ggtcacgttc ggggcgttaa tcgcctggcc tgtggtgtca gcctgaatcg
     2761 tttgtccggc ggagaggacc agtatgttct ggaggaccgt cgtggtaatg gtgtcatcgc
     2821 tcgacggtgg ccgtcccgtc accagtacgt ccacccgcat cccgggcaaa acgaatccgg
     2881 cgacgccgac cacgtcgttc acccgtacgg atacggcgcg catcccaacg ggaataatcg
     2941 gcgccaggcc cagtccgctt ccgcgtgccg cgagtctgcc ttcgaggaga ggttcctcgg
     3001 gaaggatatt gctcatcacc gcccggtcaa caacctcgtc cactcgcgaa aacccgcctt
     3061 tcggaaactg gtccgccgga attttggcaa ttttcacgtg gtcgggcttg atgctgactc
     3121 ctactccaag cgcgcctttt gaaacgacaa tgtcccgcgt ggcttgattc gctggttttc
     3181 ctgttgcggc gcgtgtcgtc atctggtaga agatgcccga aaccacgagc gcgaagacca
     3241 ggctcagagc caagaccgtc aaaaatctcc gatccatttc atcctttccg cctcacagcc
     3301 ttgccgcatt gcctagtgcc ccagaatcgc catcagtgtc ccgacgaata tagcgggagc
     3361 gtacggaatc gatgcatcct cggcatccgt ccggtgccgt ccggagagcg ccctcttcag
     3421 agcgcgtaga gcaagcacca ccagcgcgaa accggcacca gccaggaaca ccaggaccag
     3481 cgccaatatg agcgaaccgg cgcccgcaac cgcgccgaat cccgccatca acttcacgtc
     3541 gccgccgccc attccaccca gcaggtagaa caggagcagg gcggcgaacc cacccagggc
     3601 gccgccggcg gcattcaata atccccacca cgaacccgtc catgtgtgca gaagtaatcc
     3661 ggagacgact gcggcgaccg gaatccagtt cgggatctgc cgcctgcgga aatcctccgc
     3721 gctggcagca acacccagca gaagaacaat tgccaaagcg attgtcattt gggtccactc
     3781 tccgcaccgg atcccactgc caactcctcg ccggccgtta aaggtcccat cagagcgggc
     3841 gcgaggaagg catgctcgag agcgaagtcg gtgatccgct ggcccgaggg caatgtccat
     3901 cccgtgtcat cccagccggt tgtttccacc ggcgcaccca aaccgccttc caggatcctg
     3961 ttgcctctgc cgcgcggttt cgactgtctc acaacgcgtt ctctccctca caggattctt
     4021 cggcagcctc ggtacttttc attagccccg ccgcggggta ctgccaataa tgttgttgaa
     4081 ttgggggtag tgctgaggtt agaggggaat actattgctg agcggaaggg tacaggcgcc
     4141 ggaccgcgcc gctctgaatc cggccaggcg cctcaaaaca ggagagttca ggctttaatg
     4201 tcgtctctgg caaaatccca ctcgaccacc ttgttctctt tggccgagcg attaaccata
     4261 tgcgcgcagg ccgccgcgtg gtgccccgcc gcggcatctt cccaataagg ctttcgagtg
     4321 cggatcgaat cgatccagtg agagaaatga agaaccgtcg cgtccgggcc ctgttgccgg
     4381 agttgctggt tcccttcgag aacatcgggc ttccaggtgg aaggcatctc cgcctgccgc
     4441 actttcgggt cacggtaata cgcctcttcc aggcgcttgg gccagctttc cacaatccac
     4501 cggttgtctt ctaccgcaac ctccggatag agcgtggccc cgccccaccc cagattcagg
     4561 cttccctctg tgcccagaaa ttcgaaactg ctctcggctg aggtctggtt gttgaacgtc
     4621 gagctgagat tgacggcgaa tccctcggga tattcgagca ccgcgttgag agtgtccggc
     4681 acctctctgc tttctttcca gcggtacagt tgtccgagcg ccatcacttt ggaagggacc
     4741 ttcgcaccca tcaggtaatg aatggttgtg cagagatgaa cgaaaaggtc cgtcgcaatt
     4801 ccgccggagt agtcgtcgta gcagcgccac cggaaaaagc gctccaggct gaaagaccgt
     4861 ttcggggcgg ggccgaggaa catctcccaa tccacagtct gcgccgatgc gtccggggga
     4921 atcggataga tccaggctcc ggaagccgta ttgcggttgt acgcggcgcg gatcaaagtt
     4981 actttgccga tcttgccggc ctggatgatc tcccgcgctt ttttctgaat gggagagctc
     5041 atgccctggc tgcctacctg gacgatccgt ttcgcactct gcgcggcctc gatgatctcg
     5101 acaccttcgg tcgagcggta ggtcagcggc ttttcgcaat agacatcttt ttgtgccttg
     5161 atggcatcca gaaccatctg tttatgccag tgatcgggcg tcaccaccag cacggcgtcg
     5221 atggagggtt gcgcgagcac ttcccggtag tccttgaaag acttggcctt cccgccgacc
     5281 cggtccaggg cgcgctccac gcggcctttg taggcgtcaa cgacggcgac gatctcggcc
     5341 tgcggcaact gcatgagcgc atgcattaat tcatgggagc gcgctccgca tccgatgacc
     5401 ccgacctgta tgcggtcgtt ggcagatacg ctgcgattgc cctgggctgc cgcaagcgcg
     5461 gcgcctgcct gcatgaagtt tctacggcta agttccatga gctgtccctc ccatcacact
     5521 agcgggcaat ggccgaatta tacaaagggg gctttcgcgt atgaaactcc gttgcccctt
     5581 cgagcgccgt gcccaccctc aggataacgc cttcttcaaa cggccgcccg acgatttgaa
     5641 gcccgagcgg aagccccgat tccgtgaagc cgcaaggcat ggacagcgcg gggtagccca
     5701 gaacgttgat gccccgcacc agcctggttg tcgccagccg cacatcttcc tcttcaccgt
     5761 tgaacatcac cttgctctgg ccgattcgag gggccgccat cggcgtcgcc ggagtaaaga
     5821 aacagtcgat gcggctccac agcgcggaga attcggcgca aagaacgctg cgtaaacgct
     5881 gcgcattgat gtaatctgtc gccgggagaa gccggccctg gtccaacagg gccaatacgt
     5941 ccggaccgaa ctgatcgcgt tcggccatgc ggctttccag gatcgcggag gcttccgcca
     6001 acaggatcac cctgcctacc gtgttcaggg cctgaacatc ggggaagggc agccgcacca
     6061 gggatgctcc aagctgttcc gctactttgc tcatggactc cacagccgcg gcacattccg
     6121 gcgcgactcg ctcgaagtag tggttttccg gcaggccgat gcgaaggccg gcgatggagc
     6181 gattggcttc ggatagataa tccgctaccg gagcacggct ggaggttttg tcccggacgt
     6241 catgtccggc gatcgcttgc agcacgacgg cggcatcgcg cacagagcgg gtcatcgggc
     6301 ccatatgatc caggttgtag ccgagcggga aaacgccatg gcggctgaca cgtccgaatg
     6361 ttggcttcag gccgacggtt ccgcaataag aagcgggaat gcgaatcgag ccgcccgtat
     6421 cgctccccat gcccaggaaa accattccgg aggccacggc tgcgcccgat ccgccgctgg
     6481 agccgccggg aattcgttcc gggtcccatg gatttcgcac cggcccgaaa tgagggttgc
     6541 cggacgtgat gccgtaggcc agttcatgca aaccggtttt ggccagaagc accgttccgg
     6601 catcccggag ccgttccgtt acagtggcgt cgtgagcggg tacatagttc gcaaagagtt
     6661 tcgacccgca ggtcgtccgc acgcctttcg tatagaagtt gtccttcaga acgataggaa
     6721 ttccgtgcag taatcctaaa tcctttcccg ataccaactc ctcttcggcc tgtttcgccc
     6781 gtcgaagagc ctgctcctcc agcaccgtga gacaggcatt gagccggggg ttgagttcgc
     6841 gaatgcctgc gagacaccgg gacgtaagct caacggggga gagatcgcct gcacgaatcg
     6901 ccgcggctgc ttcgaggatg gtcattgggt atccggaccg cattcaaatg tgatggcagg
     6961 ttccgtcccg ggagcgagcc cgcgtaggac ggcgcaatcc ttttcaagcc gttccagtgc
     7021 ggggcaaatc ttctccagtt cgggcgcggg gattcccagg tcgagccctt cagcgatgag
     7081 cttccagttt ttcatgaaaa ttcattctac ggtgtttcgc gggccggcgc cgttgaatcg
     7141 ttccggccgg gatgacatca ttcaagaagc catgtccacc cgtatcgagc agatggagcc
     7201 ctctccgctt acccggctga gcgaagaaga agagggtttt cgtgccgaag ttcgcagatt
     7261 tgcgcgggag cagatcgcac catacgtccg cgagatggat gaaaagggcg ctttccggaa
     7321 ggagatcatc caacagtttt tcgacctggg attgatgggt atcgaaatcc ccgaggaact
     7381 gggcgggcag ggcgggacat tctttcaggc ggtgctggcg gtggaagagt tgtccgcggt
     7441 agatccctcc gccggcgtca ttgccgacgt gcagaacacg ctcgtcaaca atgcgctgat
     7501 gcgttgggcg aatgacgagc agaagcgcgc ttatctgccc agacttgccg gcaacgccgt
     7561 cggctcctat gcgctttccg aagccggcgc cggttcggat gccttcgcac tggcagctcg
     7621 cgcggaagaa aaggacgagg agtatttcct taccggccgg aagctctgga tcaccaacgc
     7681 cgcggaggcg gaactgtatc tcgtttttgc cacactggac cctggcgccg ggtatcgtgg
     7741 catcacctgc tttcttgtag aaagaggatt tccgggtttc gaggtcggca agaaggagga
     7801 caagctcggg attcgggcat cgtccacctg cgaactgatt ctcgatgctt gccgggtgcc
     7861 gaaatcgaat gtcatcggcg agcccggtaa gggatacaag atcgcgatcg agacgctaaa
     7921 cgaaggccgg atcgggatcg gcgcgcagat ggtggggctc gcgcgcggcg ccctggatca
     7981 cgctctggcg tatgcaaaac aaagaaagca gtttgggaca gctattgccg cgtttcaggg
     8041 cgtgcagttt gcgctggcgg atatggcgac ggagttggag gctgcccgcc tgctcgtgta
     8101 caatgccgct cgtttgcggg atgccggggc accgtttgtc cgggaagccg ccatggcaaa
     8161 gctgtatgcg tcggaggcgg ccgaacgaat cgcttcggca gcggtcgaag tgcttggtgg
     8221 agtgggtttt acgaaggatt acccggttga gaaactgtat cgcgatgcga agatcgggcg
     8281 catttacgag ggcaccagca acatgcagcg gctgacgatc gcgaaacaaa ttttcgggaa
     8341 acccgtttaa attcaatacg tcttacgaag aaagagggac ctatgcgcca gcttcttatt
     8401 gccgcgacgc tctgcgtgtt tctaactgga accggtatgg ctgccaaggc cgatctgagc
     8461 caagccgagg tggacgagat catcaccaaa tttgccgcaa aggaaggcga gttcgccaaa
     8521 gcacgggaga actataccta ccggcagacg gttcgaatcc aggagctgga ccctgcgggt
     8581 aatgttcaag gccggcacga gatgatttcc gacatcatct tctcagccga cggcaagcgt
     8641 acggagaagg ttgtccgggc gccggtctcc acgctcaaaa acattcttct taccccggaa
     8701 gacgagcagg acctgcgcaa cgtacagccg ttcgtgctga cgacgaagga tattccggac
     8761 tatcacatcc gttttctcgg caaagaaact ttggacgaaa ttccctgcta cgcattcgcc
     8821 gtgaagccga agcagatgga agccgggaag cggtacttcc agggaattgt ctgggtggac
     8881 gatcgcgacc tgcaaatcgt caaaacgtac gggcgcggcg tcggtcttct caagaaaaac
     8941 tccgataacc aattccccaa atttgagaca ttccgggaac agatcgacgg caaatactgg
     9001 tttccgacct atacggcagc caacgatacg ctccattttc aagctggtcc gcagcggatc
     9061 cggatgattg tccgatacga ggattacaag caattcaagg ccgacacgac aatcaaattc
     9121 ggtgaagagg tggacacgcc gccttctgtc gaagaaccga aaaagccctg atttcaccgt
     9181 tcacaaggta aaacgcacag gtcgaaacac tgtcctgcac ccgggcggct cccttttcgt
     9241 acaataaaga tctgcatcta tatgggaaca agggggttga cctttcggcg gcgggtacgg
     9301 gcgatctcga ggcgggcgcg cgaattgcgc atgatcgcca aaggggcttt ttccacggat
     9361 catccgattc ttgcccacct gattccaatc cggcgctgca acttgtcctg caagtattgc
     9421 aatgagtacg ataatgtctc caagccggta ccgacggaaa tcatgttggg ccggatcgac
     9481 cggcttgcgg ctctgggcac cacgatcatc accatcagcg gcggcgagcc gctgcttcat
     9541 ccggacctgg accagatcat cacacggatt cgccgtcgcg gaattctcgc gggcctgatc
     9601 acgaacggct acctgttgac cgccgagcga attcgccgcc tgaacaaagc cgggctggat
     9661 catctgcaaa tcagcatcga caacgtccag ccggatgagg tttcgaaaaa gagtttgaaa
     9721 gtgctggaca agaagctcca gttgctggcg ggtcatgccc tgtttcatgt gaacatcaat
     9781 tcggtggttg gcggcggcat ccggaatccc gaagatgccc ttgtggttgg gaagcgggcc
     9841 gtggaattgg gtttcacctc aacgatcggg attattcacg atggcgatgg ccagttgagg
     9901 cctctgggcg ggcgtgaacg cgaagtgtac caccagatga agcggctgga gaaaagcagc
     9961 tacgctcagg tcaactattt tcaggatgcc atcgcggaag gccgtccgaa ccgttggcgt
    10021 tgccgtgcgg gcggacggta tttgtacatc tgtgaagacg gactggtgca ctactgctcg
    10081 cagcagcggg gctacccggc caaacctctg atggaataca cggtcgaaga tatccgaaga
    10141 gaatacctta ccgaaaaagg ctgcgctccc aactgcacgg tttcctgcgt tcaccagatc
    10201 tcgtatttcg atttctggcg aggcgagcag acgattcgct ccgcggatcc ggcgccggaa
    10261 gctgctccgc tggtacacat cgagatgaac tgatttactt tcaggccctc atcagctctt
    10321 catccgcgcc atcatataaa gtccggcgag agagaagacc gcatcgggtg accaggcggc
    10381 cacttgcggc ggcagttgcg ctacgttccc catctgttcg aaaagcgagt tggttgcgag
    10441 ataagtgatc gcgatgccca ggctcacgcc gacaccagcc atggctccac gattacctgt
    10501 cacgaatgca aaaggaatcg caatcaacgc catgatgagc acaaatagcg ggaccgagaa
    10561 cttcttgtaa aactgcacct gtaagcggac cgtgtcgaag ccactttgtt gaagctcacg
    10621 aatatagccg gcgagttcgt ggaagttcat ctgcttgtcc tgctttactt ccttcaggaa
    10681 atagctgggc ggttcgttca actcgggaaa tgtggcagtc tggcctcgaa aatcgcggaa
    10741 agtctcaccg tctgtgccgc cgccaagttc ccgcatccag ccattctgga aaatccacgt
    10801 tttcagggac ggctgccaca atgccctttc ggccgagata tgccgggtca accggaacgt
    10861 accagggtcc agttcgtaca cggagatacc gcccattacg ctggagaccg ggtcgaaata
    10921 cctgtagtag taaatgcggg aaccatttcc gaaaatccat ttccggtccg gccgcaagta
    10981 cgtctggatc ggctttcccc ggatctcgtt gcggatcgca tcctgtttgc ggttcgtatc
    11041 gggaatgacg taatgatcga acgcaaagag cgcgccgctt aatcccgcgc tcatcaacag
    11101 aatggggatg gccaggcgat gcagcgatac gccgcaggct ttcatggccg ccacctcgtt
    11161 attcttggtc aaaatgccga aagtaacgag cacggccacc agtacgctca ggggtgcaag
    11221 atcgtacaac agcttcgggg tcaggaagaa cagaaacgca aatacccgct gcatgggtat
    11281 gttgttgcga atcacgtccc ccagcaattc gaagaactga tagacgtgcg tcatcagaac
    11341 aaagcttgcc aatagcagcc cgaagtagaa gagaaaaccg ttcaggatat acgtatcgac
    11401 gatctgggga agcagtggca tgcggccgcc ggagcggcgg gaaagcggcc ccggccgcga
    11461 cggcagccgg tggagcagcc ctcgcaggat gcgaccgatc caggtccatg cggtgccgac
    11521 ccaatctctg tcgccgggac gctcgaggcg ggccagcaaa atgatccccg tgatcgcaaa
    11581 cacgatattc ggcatccaga ccgcgatgcc cgccgggagc gtgccctgcc gtgctacacc
    11641 aatcaggctg atcagggcca tgtagtacgt gaatgccagc aaaaccgtca tcacgatggc
    11701 ggtggactta cctgctttgc gtgtagagac gccgagcggc aggccgacaa gcgccaacag
    11761 gatacatccg agcggtagtg cgaagcgctg atgaagctcg atgcgcgcat ccaccgactt
    11821 ctctgcttcg gccatgagcg gaaaagtcgg catctcgctg tatgctttgg ctgtacggat
    11881 ctccggcggt tcggccacca acacctggtc acctttcggg aacccggtat tgaagtattg
    11941 tgccgggtct ttcccgactt cgtaggtccg gccgttcagc atggaaacct gaatgcggct
    12001 gcgctctaca tcggcaacag cgatcgcttc cgccgcgacc gtaattcgcg gatcgtcgct
    12061 cgcctcttca gtccgcgata ttctctcgcc ggctggagtg aggtccgcca tgaagacctt
    12121 tttccaccgg gtcaccggac cgggctccac atcgccgaca tagagaatcg tgttggtgag
    12181 ggtctcttca aaaacccgcg gccggatctc ggcggtcatc tgctcggcga ccagcttatt
    12241 cagaacgcgg taggtttcct gaatcgatag cggcgtgagc catagggagg tggccgccgc
    12301 cgcagccgta gccagcgccg cgaacgccat aacgggccaa aagacccgcc ggccaggcac
    12361 gcccgccgcc ctcatggccg tgatctcgcc gtcgctcgcc atccggctaa gcccgatcaa
    12421 tgtgccgacc agaactccga ccggtatagt gaacgtcagc acaaacggaa gcaccaggat
    12481 gaagaggagt ccgatcgtgt cgatcggcat cgaactgcgg accagcagtt cgaacagccg
    12541 ccctaccctt tgcagaaaaa ggacaaaggt gaagagaacg atgccgagga tggcgcttga
    12601 ggtgatctcc cggaaaacgg ttttacccag tgtcatctgc tacaggtgag ctcgtgcagt
    12661 ttctgatgcg gtggcccctt ctgaaagaga cggcggaggc acgagcggca gtttcggtaa
    12721 ttgctcctgg gaacgctcga gctcgagttc gtcgaacagg accgagggtg cgaccacggt
    12781 tgcgggcgcc acaaacgtcg ctccgccggt ggcggcgaac ggagcggtgt tgtcgaggaa
    12841 atcgaaaacg tgattttcgc cggacaccgc gacaatgtcg cgcaggctgc gaaccgagag
    12901 gttccggaag cgcatgccgc gcaccagttc ctcgcggccg tcggcgtaaa cgcgatacac
    12961 cagaacaggg aggctcaccg gatttcgtcc gccggaggac tgagccattc cggcagccat
    13021 gcggcgaatc tcatccagcg gagccgtgga tggataatcg agttttctga cgagaagacc
    13081 gtaaggtttg ttgcgctcgc ggcacatttc gatcagcttc cgcttgagtt cctcggcgga
    13141 agtggtttgg gacgctttga taaacaggtt gccgaaagtg gcgcccttag cgccgaaatt
    13201 tccgggaagc ctcgccctgc cgttcgaacc cgagtagcct cgcaccggct ggcgggtcaa
    13261 caggatgttc ttgagtgttc ctttttcgat tatggttaag ggaaccggcg gcacgccttc
    13321 catatccacc gggtagtatc caagcagcgg cctgccgcgc cattgcgatt gtgtggggtc
    13381 gtccaccaca tccatccatt ccggcaaaac ccgcgagccg atacggcctt cgatctcact
    13441 ctggccgaga gggatggggc ggttcggctc ggaaaccgga cggcgaatca tcgccagatt
    13501 gtccccgagg acctgagcga acatctgcgg agcagcttct ccttccagaa gtaccgggcc
    13561 gatgtaactt t
//
LOCUS       DASTMK010000357        17562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_305913, whole genome shotgun sequence.
ACCESSION   DASTMK010000357 DASTMK010000000
VERSION     DASTMK010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17562
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305913"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFQ79_15730"
     CDS             complement(<1..609)
                     /locus_tag="VFQ79_15730"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0141173.1"
                     /translation="MGISTNKTNFQRAVTLFVFSGFLLNIHAQEQTGIVAGMVFDATT
                     GQAIRRVQVQVEGLPEKTAATNLDGKYRIVAPPGTYKLLFTGTNHLDTTIEQVTVVAG
                     ETVDASTVMVGKNSVTTVDVVERLSPVTSTAEAMLTERRLAPVVTDSIGSDDIRGGTA
                     SDAASALEKVTGVSIVDNGFVFVRGLGERYSATMLNNAMIPTT"
     gene            complement(654..1376)
                     /locus_tag="VFQ79_15735"
     CDS             complement(654..1376)
                     /locus_tag="VFQ79_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0141174.1"
                     /translation="MKKIVLIEDDADLFALLKYNLEKEGFQLAGAQTGKDAVELCRRE
                     RPDLILLDIMLPDSDGLDICKGIRANPNLMHVPIIFLTARASETDRIVGLELGANDYI
                     VKPFFIRELIARIKIQFRGTAAPVRVLKAGGLELDRGSCRVHLNGAELTLTATEFRLL
                     EFLMGHPGVVFSREQLLNAAWGHDRAVTDRTVDVYILRLRQKIEKDAANPIIIRSVRG
                     FGYSFDAAAEPAQALAGFVTQD"
     gene            complement(1425..2909)
                     /locus_tag="VFQ79_15740"
     CDS             complement(1425..2909)
                     /locus_tag="VFQ79_15740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0141175.1"
                     /translation="MTARIFAKLIAGVVCVLVIALFAVDFLVSRIAEDNYTETLTLEL
                     NDKARLVAELAGGPLIHLSQERARILAKAAGARLTVVDIQGHVLADSESDAATMANHR
                     SRPEIAAALNGRTGSSVRLSPTIGVNFLYVAIPMPGGALRLAVPMKDIRQTIGAVRKR
                     MLAATVFAFLPAILIAALFARHFSSRLAHIIDYAKRLSTGDFQARMEVKNKDELGILS
                     ARLNETAAKLEKMFDEIQREQAALRQLERVRTDFVINVSHELRTPLASIQGYTETLLD
                     GALDDPAHNVRFLSIIRQNAERLGRIIADLMTISRLELKTQKFQFASYSVNGLLSDAV
                     DALQPIAEKKNISLVMEPAPADAEVFCDSEAVQQILINLLDNAVKYTPQNGTITVGAK
                     PPVRRAGHDEIALYVRDNGIGIPAQDLPRLFERFYRVDKARSRELGGTGLGLAIAKHL
                     VRAQGGEIGVESELNKGSVFFFTLPVQDLGLLEAGEVQTELTAL"
     gene            complement(2937..3695)
                     /locus_tag="VFQ79_15745"
     CDS             complement(2937..3695)
                     /locus_tag="VFQ79_15745"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEU0141176.1"
                     /translation="MTNRARIKLLFAVAVLITCTSLAAFQAAQKAPGASSDPLIAGFQ
                     KTTVASVADAVDAVVGERGFMNFDMRPYSGTKFVGRARTSLVRPAPPDKATPALAVKH
                     SVEMIEEANPGDVGVIVMEKGLNVAAIGGLMGTAAKARGMAGMILDGGVRDVPELRAL
                     NLPVFARSVSPATAVGRYASVAKNVAVECAGVTVKPGDIIVAGEDGVVRVPQERAQEV
                     LKAAQEIDDRESKMVPYIQKFRSLRKAIEVFNRI"
     gene            complement(3757..4203)
                     /locus_tag="VFQ79_15750"
     CDS             complement(3757..4203)
                     /locus_tag="VFQ79_15750"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0141177.1"
                     /translation="MKSSTRRKLLTTVAAAPVALAAAQPAGGRVKQAHYAGAKPSGTP
                     LYSTAISYGDLVFISGHGVADQGGVKVQTAKVLDRIAKALESAGSSMEKVLKCNVYLQ
                     NIGDYKAMNEVYLGRFGSTPPVRTTIAAAAIPLDGCLVEIDVIAHR"
     gene            4243..5454
                     /locus_tag="VFQ79_15755"
     CDS             4243..5454
                     /locus_tag="VFQ79_15755"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0141178.1"
                     /translation="MYSRLSFPFFWLLLISTFLISCSCSTAVNKTQIETVSVQVRKPA
                     RVDRAVTIAASGTVEAGDSVDVAFQVAGRIARVIPDEGSFIRKGELLAQIDATDYNLG
                     AEAAQAQTAMARANHEKARAGARAQELEQARIAYERAADEHRRMRILYERKSLAPNDF
                     RKFEAAWQLARERYDEAKEGARGEDKAAALAALEQAQAAEKIARKRVADTALYAPLSG
                     FIARRGVDAGETVPAGAPVFTIMNVDPAKVRVGIPETDVSKVRVGQRANVSLPALPQA
                     VFTGRVTLIGVAADPMSRTYTAKIEVPNRGHSLRPGMIAEAQIESNVRTGAITLPGQA
                     ISRDPQGVTVVFLYFPDQQRAHMRRVETGTAYGSEVEIRSGLTGDELVVVAGQQKLRE
                     GSTVHVVGDVQ"
     gene            5451..9842
                     /locus_tag="VFQ79_15760"
     CDS             5451..9842
                     /locus_tag="VFQ79_15760"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0141179.1"
                     /translation="MNPVKASLAYPQVTLILTAMAVAVGVHALLTMPRREDPKITIRT
                     GLVSAIFPGASAEEVENQVTRKIEDRLFRFEEVRKSKTYSTSRNNIVFVNVELEDWVQ
                     NPDQFWDTLRHDLNELRVRELPPGVQGPVVNSDFGDTVAVLLAVHGGRYDYRQLKGYV
                     ERIEDELRTIRAASKLKRYGEQKEQIYITSSFDRISQYATNPLRVIQALQARNTVQYA
                     GSIEAGHSDVPLKTTGPFQTEDQIRRVMVDVSRTTGQPVYIRDLAEVERRYQDPQYLT
                     RFNGQRAVMLSVEMQKGYNIVDFGKEIRAHLDRLRTLLPPDLEIDLVADQPRIVEQRI
                     SHFIREFGIAIASVILVTLVLLPFHVAAIAAFAIPVTIAITFGIMNAVGGELHQVSIA
                     ALIVVLGMVVDDAIVIADNYVELLDQGVPRQEAAWRCATELAVPVLTATLTIIASFVP
                     LLMLTGSVGEFISALPIAVTVSLGTSYVIAMLLTPLLCRWFIKKGLHSPEKDTAGKKK
                     FSVLDRMQTYYGSTIRLMMRRKPLAAGLGAAAFACGVILIQIAPEQFFPSAERDQFVI
                     DLWMPEGTRIEATDAVLRRMENHLASQKLVATFASFLGQSAPRFYYNVSPEQPAANYG
                     QLLVNTNSVKETPALVHRLREEFSTLAPEGIVIVKELQQGQVYQAPVEVRIAGDDITA
                     LKTLGSKVEDILRSSQSSAYVRNDFHEDTFQVRVKVNEEVSNRLGLTNASVSQQLAGG
                     FSGAPVTTFWEGSRAVEVLLRLEEGRRKQFEDIENAYMTSLVTGARVPLRQVATVDPE
                     WQTSRIVRRNGVRTLTVQSFFKEGHLASEVLKDAQPAIDDIALPPGYRIDYGGEIESQ
                     TETFSEMVRALLVSLLAIFLILLFQFRSITDPLIVMVSIPLALLGAAAGLFLTQNPFG
                     FTAFMGIVSLSGVVVRNAIILVDYINERLRDGHDLEEAAMEAGQRRLRPIFLTSMAAA
                     VGVTPMILSRSSLWSPLASVIAVGLLFSMFFTLLVVPVLFVLIRRRRHASGGALAVLL
                     LVLLTPGLHAQTRRLTLPEAVDTALRQNVMLKIARLKVEENRHKTGAARANYFPRATN
                     ESNLLNTVERQKIAIPAGIFGVFQDEGPVPPRPIPIEQGSSNLFYTTTTLGQPLTQLV
                     KIRAGHGVALADAAAAAADLKKAENEIALKVHELYFGLLIARAQKSAAQAQVEFAEAR
                     MTDSRAAVEAGNVLEVALIGARAGLLENRHQLLAADDQIADLAVEFNDLLGLPLDTVG
                     ELVPPDPVDTALNDRGEYTRIALAESPDVHAAEEAVEKARRGVTAAKADYIPELSAFV
                     QHVYQNGVPFLARNNGILGLRMNWDLFDFGKRRSAVGEREALLAQAEENLRRQKSRVS
                     VEVEKAVRKLERARQMLEVAREALHLRRENERLTADQVEVGVASASAASETRAAAAKA
                     EADLLRANLGYLLAHAELERTIGRTPR"
     gene            complement(9843..10916)
                     /locus_tag="VFQ79_15765"
     CDS             complement(9843..10916)
                     /locus_tag="VFQ79_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0141180.1"
                     /translation="MPDYPVSRRAFVAGAASLPVTAAPSLQSVSTTRSGPVRIVTMYP
                     FEAHEAAKIRAAASNAKVDLILCKDRDEFRKNLREADVVYGALGGADLDYAPKVKWVQ
                     AGGAGVENLDKRFRESPIVLTNFARTFAPGISETGIGLLLCLTRGISKYYMPQFYKRQ
                     MEPVGTPKSDHHTELKGRTMGVVGMGGIGSAIARRAYYGFDMRIVGTDAKPMPAPEYV
                     AELRDPGWFEEMVPQVDVLVASAPHTPETERMFNEKIFRSMKKTAYFLGLSRGMLFDD
                     MALVRALKEGWIAGAGLDVFPVEPPPSSHPIYDCPNVVMTAHTSGWSPDRQVRLIDLF
                     AENVRRYAAGLPLINVVDKQKGY"
     gene            complement(10945..11532)
                     /locus_tag="VFQ79_15770"
     CDS             complement(10945..11532)
                     /locus_tag="VFQ79_15770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU0141181.1"
                     /translation="MKAIFWDVDTQLDFLYPAGALYVPGAERIVDTIARLNHYAAANG
                     MHVVSDVDAHAEDDPEFLHWPPHCVAGTAGQQKPQSTLLDHRAVVPFKSGEFPIDKVQ
                     QLILEKPTLSCFADPNLPPLLQALNPDKYVLYGVVTEICVRHAALGLLDTGKRVELVT
                     DAVRHLNEQDRDRFFEEFTARGGKLTTADEVCSTR"
     gene            complement(11529..12875)
                     /locus_tag="VFQ79_15775"
     CDS             complement(11529..12875)
                     /locus_tag="VFQ79_15775"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU0141182.1"
                     /translation="MQGLLTDLYQLTMAAGYYQAGKVHEKATFELFVRKLPHNRNFLI
                     AAGLEQAVDYLLNLRFTEEEVGYLRGLKQFAQVPKDFFEALRAFRFTGDVFAAAEGTP
                     MFAGEPLLTVRAPLIEAQIPETYLLATIGFQTMIATKAARMTEAACGRSVVEFGTRRA
                     HSPEAGVLAGRAAYVGGCIGTSNTLAGFRYNIPVFGTAAHSWVQAFPAELDAFRELQK
                     LLGPATVYLIDTYDTVEGARRAAQLGEPVWGVRLDSGNMKELSQAVRQILDDAGLKSA
                     KIMVTGDLNEYKILELMAAKAPIDSFGVGTDLATSADAPNLGAVYKLVELESNGQKRY
                     TAKFSENKLTMPGAKQVFRFDDHDEIACSWECRGCPSTGPAARALLRPVILGGRLVEA
                     LPSATAARENACDSLKKLPGVYRRLFAAEEPYPVRYTPALNVLLEQVRKEREGVPA"
     gene            complement(12885..14222)
                     /locus_tag="VFQ79_15780"
     CDS             complement(12885..14222)
                     /locus_tag="VFQ79_15780"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEU0141183.1"
                     /translation="MGSRGLLIGAVVLAILAGGVYWSNKVEEKKAAQPAADAGPKILE
                     IPEEQIQQVEIKRKDGPETIIRKTDAGKWEITSPKPLAADEDAVRSLLSTVSSLSSDK
                     LVEEKASDLGPFGLDAPTMNVVITKKGGDSRTVLVGDETPTGSAAYVKLENDPRIFTI
                     ASWNKSSLDKTAADLRDKRLLRFDQDKLSRVELVRKGQSVEFGKNNKNEWTIIRPSPM
                     RADGGEIEELVRKLRDAKMDTSLSAEDDRKTAASFAGGAQVAVVRVTDAAGTEQMEVR
                     KNKEDYFARSSVLEGTHKINADLGQAVDKDLEGFRNKKLFDFGWNEPSKVQVKDGDKT
                     MVFEKSGDKWMSGAAEIDSTSVQNLIDKIRDLSAVKFVQQGFTAPVFEITVTSNEGKQ
                     TETVEISKAGESHIAKRANEPTLYEVDAATVEELRQAAGDVKPAQAAKDEKKK"
     gene            complement(14226..15602)
                     /locus_tag="VFQ79_15785"
     CDS             complement(14226..15602)
                     /locus_tag="VFQ79_15785"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEU0141184.1"
                     /translation="MKTDWIKTRQTRYTAYATLYIVIIIGVLALVNFLANRHNKSYDS
                     TANKRFSLSDQTEKVVKNLKQDVTVSYYDVSSRFTSAQDLLGRYDNLSPKLSVEYVDV
                     LKNPQRARAEGVSTEGTVFVKAGDRKEEARSLTEEEITGALIRALKTGTRSVCFVQGS
                     GEHSISETNRDGYSQFKDALEKSNYQTRTISLLEKPEVPSDCTIVVVGGPRFDYVEPA
                     VNALRAYVEGGGSALFMIDPPLRMGRENISDNPGLVKLLESWGVKAEKDLVLDTSGFG
                     QIFGLSAAVPLVVNYESHAIVREMKEVATAFPLSRSLDTTTAEKTSVDKLFSTSNNSF
                     ATTNLSSAEIQPNEAKDKKGPLTLGVAGTYDTGKENQKGRFVVTGSSGWVTNGILRFN
                     GNRDLALNMINWLSADEDLISIRPKEPEDRRLTLTGSQMRVVFYSSVVFLPLLIIAAG
                     LSVWWRRR"
     gene            complement(15603..16367)
                     /locus_tag="VFQ79_15790"
     CDS             complement(15603..16367)
                     /locus_tag="VFQ79_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0141185.1"
                     /translation="MKNIWIICRKELKSYFASPIAYGLMAFFAAISGYFFYVITAVFV
                     QRGIQSQMMGQSFPMDVNEWVVRGFLSNVSVIGLFLIPMITMRLFAEEKRTGTIELLV
                     TSPVKDIEVIVGKWLAAVILYAGILLISALNLAILFLYGKPDWKPILVGYLGLLLLGG
                     ALLAIGTFISTTTKNQIIAGTATFAVCLLLWVFDWVSYETTTWAKVMSYLSVVAHFEP
                     FSKGVLDSKDIIFYLSMIFFGLFLTARSLESLRWRS"
     gene            complement(16364..17338)
                     /locus_tag="VFQ79_15795"
     CDS             complement(16364..17338)
                     /locus_tag="VFQ79_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0141186.1"
                     /translation="MIKVEGLSKRYARTVAVDQISFEVNKGEIVGFLGPNGAGKTTTM
                     RILTCFLPPTAGGASVAGFDVLTQPLEVKKRIGYLPETPPLYPEMEVVEYLHFVGRLK
                     GIPKSDIAARVDEVSERCSVTDVRTKLISKLSKGYRQRVGLAQAIIHNPDVLILDEPT
                     AGLDPKQILETRQLIKSLSGAHTIILSTHILPEVEQTCEKVIIISKGKVVATDSVHNL
                     TNRLRGSEAVGLEIEPPQGSTGSAEIQQRLEQVPGVSRVLLRDSRDGRYTFEVESLQG
                     RTVRGDLARSVVEAGWNLNELRPVALSLEEIFLQLTSSDATQSPVGAK"
BASE COUNT         3556 a         5412 c         4956 g         3638 t
ORIGIN      
        1 ggttgtcgga atcatggcgt tgttcagcat ggtggccgaa tacctctcgc caagaccgcg
       61 aacaaagaca aacccgttgt ccacaatcga taccccggtc accttttcca gtgcgcttgc
      121 cgcgtctgac gcggtaccac cgcggatgtc gtcgctaccg atggaatccg tcacgacggg
      181 agcgagcctg cgctccgtga gcattgcttc ggcggtcgat gtcaccggac tcaaacgctc
      241 gacgacgtcc accgttgtga ccgaattctt gccgaccatc accgtgcttg cgtcaacggt
      301 ctcgcccgca accaccgtta cctgctcaat cgtcgtatcg aggtgatttg ttcctgtaaa
      361 cagcagcttg taagtgcccg gaggcgcgac gatgcggtac tttccatcca ggtttgtagc
      421 ggccgttttc tcgggaagtc cctccacctg cacctgcacg cggcggatgg cctgtccggt
      481 ggtcgcatcg aaaaccattc cggcgacgat tccggtctgc tcttgagcgt gaatgttcag
      541 caggaatccg ctgaatacga aaagcgttac cgcacgctga aaattcgttt tattggttga
      601 tatgcccaaa gccgagagta tcaaccggct gtaacgatac tattgctgcg ctattaatct
      661 tgcgttacaa aacccgcaag ggcctgcgcc ggttcggctg ccgcgtcgaa gctgtatccg
      721 aaacctcgca cggagcggat gatgatggga ttggcggcgt ccttctcgat tttctgacgg
      781 aggcggagga tatagacatc gacggttcga tccgtgacgg cccggtcatg tccccaggca
      841 gcgttcaata gttgctcgcg gctgaaaacc actccgggat ggcccatcag gaattcgagt
      901 aaccggaact cggtggctgt caatgtcaac tcggcgccgt tcaggtggac gcggcaactg
      961 ccgcggtcca gttccaggcc tccggctttg agcactcgta cgggggccgc cgtgccgcgg
     1021 aattgaatct tgatgcgggc aatcaattcg cgtatgaaga acggcttcac gatgtagtcg
     1081 tttgcaccga gttccaggcc aacgatgcga tcagtctccg aagcccgtgc ggtcaggaaa
     1141 atgatgggga catgcatcag atttggattc gcgcgaatac ctttgcagat atcaagtccg
     1201 tcggaatcgg gaagcatgat atccaacaga atcaggtccg gtcgttcccg gcggcacaac
     1261 tccaccgcat ctttgcccgt ctgggcgccc gccagttgaa acccttcttt ttccaggttg
     1321 tatttgagca gggcgaacag atccgcatcg tcttcaatca gtacgatttt tttcatttca
     1381 agtcggctca cctttagggt aatccgggaa ttgttgcaat aacattacaa tgcggttaac
     1441 tctgtttgaa cttctcccgc ctcgagcagt cccagatcct gaacgggcag agtgaagaag
     1501 aaaaccgatc ccttgtttaa ctcgctttcg acgccgatct cacccccctg cgcccgcacc
     1561 aggtgtttgg cgattgccag acccaaaccg gttccgccga gctcgcgcga ccgggccttg
     1621 tccacgcggt aaaacctttc aaacaggcgc ggcaaatcct gggccggaat gccaatcccg
     1681 ttgtcgcgca cataaagggc gatctcatcg tgtccggcgc ggcgcacagg cggctttgcg
     1741 cccaccgtaa tcgtgccgtt ctgaggggtg tattttaccg cgttgtccaa caggttgatc
     1801 aggatctgtt ggaccgcctc cgaatcgcaa aaaacctcgg catccgccgg cgccggttcc
     1861 atcacgaggg aaatattctt cttctccgca atgggctgca aggcatcgac agcatccgag
     1921 agaagcccgt ttacggaata cgaggcaaac tggaactttt gcgtcttcag ttccaggcgc
     1981 gagatggtca tgaggtcggc gatgatgcgc ccgagccgct ccgcgttttg ccggatgatc
     2041 gaaagaaacc gcacgttgtg cgcagggtcg tccagcgcac cgtcgagcag cgtttcggtg
     2101 tagccctgaa tggaggcaag cggtgtgcgc agctcgtgcg aaacattaat aacgaaatcc
     2161 gttcggacac gctcgagttg ccggagcgct gcctgttcac gctgaatctc gtcaaacatc
     2221 ttctccagct tggccgcggt ctcattcagc cgggcgctaa gaattcccag ctcgtcctta
     2281 tttttcactt ccattcgggc ctggaagtcc cccgtcgaga gccttttggc gtaatcgata
     2341 atgtgagcga gacgcgaaga gaagtgccgt gcaaaaagcg ccgcgatgag gatggccggc
     2401 agaaacgcaa acacggtagc ggcaagcatt cgcttgcgca cagcgccgat ggtctggcga
     2461 atgtccttca tgggaacggc caggcgcagc gctccgccag gcatgggaat ggcgacgtat
     2521 aggaagttaa cgccgatcgt cgggctcagg cggacactcg atccggttcg cccgtttagc
     2581 gccgcagcga tctcagggcg gctccggtga ttggccatcg ttgcggcatc cgattcggaa
     2641 tccgccaata cgtggccctg aatatcaaca acggtaaggc gcgcgccggc cgctttggcc
     2701 agaatacgcg cgcgctcctg cgaaaggtgg atcagtgggc cgccggccaa ctccgcaacc
     2761 aatcgcgctt tgtcgttcaa ctccagcgtc agcgtttcgg tgtagttgtc ttcggcaata
     2821 cgggaaacca gaaagtcaac ggcaaacagg gcgatgacca ggacgcacac gaccccggcg
     2881 atcagcttgg cgaaaatcct cgcggtcaat agtctaatta tcccagatcc gagatatcag
     2941 atccggttaa acacttcgat ggctttccgc agggagcgga acttctggat gtaaggcacc
     3001 atcttacttt cgcggtcgtc gatctcctgc gccgccttca gaacctcctg cgcgcgctcc
     3061 tgcggcactc gtaccacacc gtcctcgccg gcaacaataa tgtcacccgg ctttacggtg
     3121 actccggcgc attccactgc aacgtttttc gccacgctgg cataacgccc tacggcggtg
     3181 gccggggaga cgctgcgagc aaatacgggc agattgaggg cgcgaagttc aggcacatcg
     3241 cgaactccgc cgtccaggat catgccggcc atgccgcgtg ccttggcggc ggtgcccatc
     3301 aggccgccga tggcagcaac gttcaagccc ttctccatca cgatcacgcc gacgtccccg
     3361 gggttggcct cttcaatcat ctctaccgaa tgcttcacgg cgagagccgg tgtggcttta
     3421 tcgggcggcg ccggccgcac aagcgacgtg cgcgcgcggc cgacaaattt tgtcccggaa
     3481 tacggccgca tgtcgaagtt catgaagccg cgctcgccca ccaccgcgtc cacagcatcc
     3541 gcaaccgagg ccactgtggt cttttggaag cctgcaatca gcggatcgga ggatgctccc
     3601 ggcgctttct gcgccgcctg gaacgcggct agcgacgtgc aggttataag aaccgcaaca
     3661 gcgaaaagga gtttgattct ggctcgattc gtcatttccg ttcgtgtcct cgatcttgat
     3721 tcccgctcat catacaacat cggcggaaga cgatccttat ctgtgggcga tgacgtcaat
     3781 ctccaccagg cacccatcca gcgggatcgc cgccgcggca atcgtcgtcc tcacgggtgg
     3841 cgtggatccg aagcggccga gataaacttc attcatcgcc ttgtaatcgc cgatattttg
     3901 caaatacaca ttgcacttca agaccttctc catcgacgag ccagccgact cgagcgcctt
     3961 ggcgatccga tccagaacct tcgctgtctg cacctttact cctccttgat cagcgacgcc
     4021 atgaccggaa atgaagacca ggtctccgta ggaaatcgcc gtcgagtaga gcggcgtacc
     4081 cgagggcttc gcgcccgcat aatgcgcctg cttcacgcgt ccgccggcag gttgtgctgc
     4141 cgcgagagca actggtgcgg cggcaaccgt agtgagtagc tttcttcttg ttgagctttt
     4201 cattttgtgg atcccatcca gataacatac aaaagacgct ctatgtactc cagactttcg
     4261 tttccatttt tttggcttct tctcatttcc acattcctca tctcctgttc gtgctcaaca
     4321 gcagtaaaca agacgcagat tgagacggtc tccgtccagg tgcgaaaacc cgcccgcgtc
     4381 gaccgtgccg tcaccattgc agccagcggg acggtagaag cgggtgattc tgtcgatgtt
     4441 gcctttcagg tagcaggtcg catcgcacgg gtgatcccgg acgaaggctc gttcatccgc
     4501 aagggagagt tgctggcaca gatcgacgcc accgactaca accttggcgc cgaagccgca
     4561 caggcgcaaa cggcgatggc gcgcgccaac catgaaaaag ctcgggccgg agcgcgggcg
     4621 caggagctcg agcaggcgcg cattgcctat gagcgcgcgg ccgatgagca ccgccgcatg
     4681 cggatattgt acgaacgcaa gagcctggcc ccgaacgatt tccgcaagtt cgaagcagcc
     4741 tggcagcttg cgcgcgaacg ctacgacgaa gcgaaagagg gcgcgcgagg ggaagacaaa
     4801 gcggcagctc tggcagcgct cgagcaggcc caggcggcgg aaaaaatcgc acgcaagcgt
     4861 gtagccgaca ccgcgcttta cgccccgctc agcggcttca tcgcgcgaag aggcgtcgat
     4921 gcgggcgaga cggtgccggc gggcgctccg gtcttcacca tcatgaacgt cgatcccgcc
     4981 aaagtacgcg tgggtattcc cgaaaccgac gtctcgaaag tacgcgtcgg gcagcgggcg
     5041 aatgtctcac tgcccgcgtt gccacaagcc gttttcaccg ggcgcgtgac gctgatcggt
     5101 gtggccgccg acccgatgtc gcgcacctat accgcgaaga tcgaggtgcc caaccgtggg
     5161 cattcgctgc gtccgggaat gatcgccgaa gcacagatcg agagcaacgt cagaaccggg
     5221 gccattacgc taccgggcca ggcgatctcc cgcgatccgc aaggcgtcac cgtcgtgttt
     5281 ttgtacttcc ccgatcagca acgcgcgcat atgcgccgcg tcgaaacagg cacggcgtac
     5341 ggcagcgagg tggagatccg gagcggcctc accggcgatg agctggtagt ggtagccgga
     5401 cagcagaagc tacgcgaagg ctcaacagtc cacgttgtgg gagatgtgca gtgaatccgg
     5461 tcaaggcatc gctcgcttac ccgcaggtga cgctgatcct tacggcgatg gctgtcgccg
     5521 taggtgttca tgcgctgctc accatgccgc ggcgcgagga tccaaaaatt accatccgca
     5581 ccgggctcgt ctccgccatc ttcccgggcg cctccgccga ggaggtcgag aaccaggtta
     5641 cacgcaagat cgaagaccgg ttgttccggt ttgaagaagt ccgcaagtcg aaaacctact
     5701 ccaccagccg caacaatatt gtcttcgtca atgtcgagct cgaagactgg gtccagaatc
     5761 cggaccagtt ctgggacact ctccgccacg atttgaacga actgcgggtg cgggagttgc
     5821 cccctggtgt gcaaggcccg gtcgtgaact ccgacttcgg cgataccgtc gccgttctgc
     5881 tcgccgttca tggaggccgc tacgattacc ggcaattgaa gggatacgtc gaacgcatcg
     5941 aagacgagct tcgaaccatc cgcgccgcct cgaaactgaa gcgctacggc gagcagaagg
     6001 aacagatcta catcaccagc agtttcgacc gcatctccca gtacgccacc aacccgcttc
     6061 gcgtcataca ggcgttgcag gctcgcaaca ccgttcagta tgccggcagt attgaagccg
     6121 ggcacagcga tgttccgttg aaaaccaccg gtccgttcca gaccgaagac cagatccggc
     6181 gcgtgatggt ggacgtttcg cgcacgaccg ggcagcctgt ttacatccgg gacctggcgg
     6241 aagtggagcg ccgctaccag gacccgcaat acctgacgcg tttcaacggc cagagggcgg
     6301 tgatgctttc cgtagagatg cagaaaggct acaacatcgt ggatttcggc aaagaaatcc
     6361 gcgcgcatct cgaccggttg cgaaccctgc tgccgcccga tctggaaatc gatctcgtcg
     6421 ccgaccagcc gcgcatcgtc gagcaacgca tctcgcattt catccgggag ttcggcatcg
     6481 cgatcgcatc ggtgatcctg gtcacgctgg tgctgttgcc ttttcacgtg gctgccatcg
     6541 ccgcgtttgc catcccggtg accattgcca tcacattcgg catcatgaac gccgtgggtg
     6601 gggaactgca ccaggtttca atcgccgcgt tgatcgtggt gctcggcatg gtggtggatg
     6661 atgccatcgt aattgcggac aactacgtcg aactgctgga ccagggcgtg ccgaggcagg
     6721 aggccgcctg gcgctgcgca accgagctcg ccgtgccggt gctgacggcc acgctgacca
     6781 tcattgcttc gtttgtgccg ctgctgatgc taacgggttc ggtgggcgag tttatttcgg
     6841 cgctgcccat cgcggtcacc gtttcactcg ggacgtcgta cgttatcgcc atgctgctga
     6901 ctccgctgct gtgccgctgg tttatcaaaa agggtctgca ttcaccggag aaagacaccg
     6961 cggggaaaaa gaagttcagc gtgctggacc ggatgcagac gtattacggc agcacgatcc
     7021 gcctcatgat gcggcgcaag ccgctggcgg ccggcttggg agcagcggcc ttcgcgtgtg
     7081 gagtcattct catccaaatt gctcccgagc agttttttcc gtccgccgag cgggaccagt
     7141 ttgtcatcga tctgtggatg ccggaaggca ctcgaattga agctactgac gccgtgttgc
     7201 ggcgcatgga gaatcacctg gcatctcaga agctggtggc tacattcgcc agctttctgg
     7261 gccagagcgc accgcgtttc tattacaatg tcagtcccga acaaccggcc gccaactacg
     7321 gacagcttct cgtcaacacc aactccgtga aagaaacacc cgccctggtg catcgtctgc
     7381 gggaggaatt ctccacgctg gctccggaag gcattgtcat cgtcaaagag cttcagcagg
     7441 gccaggtcta tcaggctccg gtcgaggtcc ggatcgccgg cgacgatata acggcgctga
     7501 agacgctcgg cagcaaagta gaggatatct tacgtagctc gcagagctcc gcttacgtcc
     7561 gcaacgactt tcatgaggac acgtttcagg tgcgcgtgaa ggtaaacgag gaagtgtcca
     7621 accgcctggg ccttaccaat gcctcggtct cacagcagct tgccgggggc ttctcaggcg
     7681 ctccggtcac cacgttttgg gaaggaagcc gcgcggttga ggtcttgctt cgcctggaag
     7741 agggacggag aaaacagttc gaagacatcg agaacgccta catgacttcg cttgtaacgg
     7801 gtgcgcgggt tccgctgcgg caggtggcaa ccgtcgatcc ggagtggcaa accagcagaa
     7861 tcgtgcggcg aaacggtgtg cggacgctca ctgtccagag ttttttcaaa gagggacatc
     7921 ttgcctccga ggtgctcaaa gacgcacagc cggcgatcga cgatatcgcg ttaccaccag
     7981 gctatcggat cgactacggc ggcgaaatcg aaagccagac agagacattc tcggaaatgg
     8041 tgcgcgcact cctggtcagc ttgctggcga tctttctgat tctgttgttt cagttccgtt
     8101 ccatcaccga tccgctgatc gtgatggttt ccattcctct tgctctgctc ggggcagcgg
     8161 caggactctt cctcacgcag aatccattcg gttttaccgc attcatgggc attgtgagcc
     8221 tttccggagt ggtcgtgcgc aacgccatca tcctggtgga ctacatcaat gagcggttgc
     8281 gcgacggcca cgacctggag gaggccgcga tggaggctgg acagcggcgc ctccggccta
     8341 ttttcctgac gagcatggcc gccgctgtcg gagtgacgcc aatgatcctt tcccggtcca
     8401 gcctctggag tccgctcgcc agcgtgatcg cggttggcct gctgttctcc atgttcttta
     8461 cgctgctggt cgtgccggtg ctattcgtgc tgatccggcg caggcggcat gcctccggcg
     8521 gcgcactcgc cgtcctgctg ctggtcctgc tcacgccggg cctgcacgcc cagacgcggc
     8581 ggctcacgct ccccgaagcg gtagacacag cgttgcgcca gaatgtgatg ttgaagattg
     8641 cccgcctgaa ggtggaggaa aacaggcata aaaccggcgc cgcgcgggcg aactatttcc
     8701 cgcgcgccac caacgagtcc aatctcctga acacggttga gcggcaaaag atcgcaatac
     8761 ccgcaggcat tttcggggtc ttccaggacg aaggaccggt accgccgcgt ccgattccta
     8821 tcgaacaggg atcatcgaat ttgttttata ccaccacgac gctgggccag ccgctgacac
     8881 aactggtgaa gatccgcgcc gggcatggcg ttgccctggc ggatgccgcc gccgcggcgg
     8941 ccgatctgaa aaaggccgag aacgagatcg cgctgaaggt ccatgagttg tacttcggcc
     9001 tgttgatcgc gagagcgcag aaaagtgcgg ctcaggctca ggtggaattt gccgaagcgc
     9061 gcatgacgga cagccgcgcc gccgtcgaag ccggcaacgt tctggaagtg gcgctgatcg
     9121 gtgctcgtgc tggcttgctg gagaatcgcc atcagctttt agccgccgat gaccagatcg
     9181 cggacctggc ggtcgaattc aacgacctgt tgggactgcc tctcgacacc gtcggggaac
     9241 tggttccgcc cgatccggtc gataccgccc tgaatgaccg tggcgagtac accagaattg
     9301 ccctggccga aagtcccgac gtacacgccg cggaggaggc ggtagagaaa gcgcgccgcg
     9361 gcgttactgc cgcaaaggcc gattacattc cggagttgag tgcatttgtg cagcacgttt
     9421 accagaacgg tgtacccttc ctggcccgca acaatggaat cctcgggctg aggatgaact
     9481 gggacctgtt cgatttcggc aagcgccgca gtgccgtcgg cgaacgtgaa gcgctgctgg
     9541 cacaggcgga agagaacctg cggcgtcaaa aaagccgggt gtccgtggaa gtggaaaagg
     9601 ctgtccgcaa gctcgaacgg gcccgccaga tgctggaggt ggcccgcgaa gcgttgcacc
     9661 tgcggcgcga gaacgagcgg ctcacggcag atcaggtaga ggtgggcgta gcatccgcct
     9721 ccgccgcgag cgagacccgc gcggccgccg caaaagccga agcggatctg ctgcgtgcaa
     9781 acctgggata cctgctggca cacgccgaat tggagcgcac cataggccgg accccgcggt
     9841 aatcagtagc ctttctgctt gtccaccacg ttgatcagcg gaaggcccgc ggcataccgc
     9901 cgcacgttct ccgcgaacag atcgatcagc cgcacctgcc ggtccgggct ccaacccgag
     9961 gtatgagcag tcatcaccac gttcgggcaa tcgtagatcg gatgcgaaga gggcggaggc
    10021 tccaccggaa aaacatccag ccccgcgccg gcgatccagc cttccttgag cgcccgcacc
    10081 agggccatat cgtcaaacaa catgcctcgc gacaggccga gaaaataggc ggtcttcttc
    10141 atgctccgga aaattttctc gttgaacatg cgctcggttt cgggcgtatg cggcgcggaa
    10201 gccaccagca catccacttg tggaaccatc tcttcgaacc agcccggatc gcgcagctcc
    10261 gcgacatatt ccggagcagg catcggcttg gcatccgtac ccacaatgcg catgtcgaag
    10321 ccgtagtacg cgcggcgggc gatggcgctt ccgatgcctc ccatgccaac gacgcccatc
    10381 gtgcggcctt tcagttccgt atgatggtcc gacttgggcg ttcccaccgg ctccatctgc
    10441 cttttgtaaa actgcggcat gtaatacttg ctgatgccgc gcgtcaggca gagcagcaat
    10501 ccgatgccgg tttcggagat gcccggcgca aacgtgcgtg cgaagttcgt cagaacgatc
    10561 ggactttcgc ggaatcgctt gtccaagttc tcgacgcccg cgccacccgc ctgtacccat
    10621 ttcactttcg gcgcatagtc cagatcggcg ccgcccaaag cgccatagac cacgtccgct
    10681 tcacgcaggt ttttccggaa ttcgtctcgg tctttacaca ggatcagatc gactttggcg
    10741 ttggaagcag cagcccggat ctttgccgct tcgtgcgcct cgaacgggta catcgtgacg
    10801 atgcggaccg gcccgctgcg ggtggtggaa acggattgga gcgagggcgc tgcggtaacc
    10861 ggcaaggacg cggcgccggc gacaaatgca cggcgcgaga cggggtagtc aggcatgatt
    10921 ttctcccaga acgatcgagc cgcttcagcg ggttgagcaa acttcgtcgg ccgttgtcag
    10981 ctttccgcca cgggcggtga actcttcgaa gaaccggtcc cggtcctgtt cattcaggtg
    11041 ccgcaccgca tccgtaacca gttccacgcg cttgcccgta tcgagcaggc cgagagcggc
    11101 gtgacgcacg cagatctcgg tcaccactcc atacaaaaca tacttatcgg ggttgagggc
    11161 ctgaagcagg ggaggcagat tcgggtcggc gaaacaactc agcgtgggtt tttccaggat
    11221 caactgctgc actttatcaa tcgggaactc tcccgatttg aatggcacca ccgcgcggtg
    11281 atccaacagg gttgactggg gcttctgctg tccggccgtg cctgccacac aatgcggcgg
    11341 ccagtggaga aactcggggt cgtcctcggc gtgcgcatcc acgtcggaca cgacatgcat
    11401 gccgttggcg gcagcataat gattcaaccg tgcgatggta tcgacgatcc gttccgctcc
    11461 gggaacatat agcgctcccg ctggatacag aaaatcgagc tgcgtatcga cgtcccagaa
    11521 gatcgctttc atgccggcac tccttccctc tctttgcgaa cctgttccag aagcacgttc
    11581 aacgccggag tgtaccgcac cgggtacggc tcttccgctg caaaaagccg cctgtaaacg
    11641 cccggcagct ttttcaggga gtcacaggcg ttttcccggg cggccgttgc cgacggaagc
    11701 gcctccacca gccgtcctcc cagaatcacg ggccgcaaca gcgcccgtgc ggccggacca
    11761 gtagagggac agccccggca ctcccaggaa catgcgattt cgtcatgatc gtcgaaccgg
    11821 aacacctgtt tggcacccgg catcgtcaat ttattttcgc tgaatttagc cgtgtaccgc
    11881 ttttgcccgt tggattcaag ttccaccagc ttgtacaccg ccccaagatt cggcgcgtcg
    11941 gcggaggtgg ccaggtcggt gccaacaccg aacgaatcga tcggcgcttt ggccgccatc
    12001 agctcgagaa ttttatactc gttcagatca ccggtaacca tgatcttcgc gcttttgaga
    12061 ccagcgtcgt ccagaatctg ccgcactgcc tgcgacagct ctttcatgtt cccactatcg
    12121 agcctgacgc cccatacggg ctctcccagt tgagcggcgc ggcgcgctcc ttccaccgtg
    12181 tcgtatgtat cgatgaggta aacggtggcc ggacccaaaa gcttttggag ttcccggaaa
    12241 gcgtccaatt cagccgggaa cgcctgcacc caggaatgcg ccgccgtgcc aaacaccggg
    12301 atgttatagc ggaacccggc cagagtgttg ctggttccaa tgcagcctcc cacgtaggcc
    12361 gccctgccgg ccagaacacc cgcttccggc gaatgcgcgc gccgcgtgcc gaactccacc
    12421 accgagcggc cacacgctgc ctccgtcatg cgagccgctt tcgtggcgat catggtttga
    12481 aacccgatgg tcgccaacag gtacgtttcc ggaatctgcg cttctatcag cggcgcgcga
    12541 accgtcaaca gaggctcccc ggcgaacatg ggtgtgcctt ccgccgccgc gaagacgtca
    12601 cccgtgaacc ggaacgcacg gagtgcctcg aaaaaatcct tggggacttg cgcgaactgc
    12661 ttcagcccgc gcagatagcc gacttcctcc tccgtgaacc gaagattcag caggtagtca
    12721 accgcttgct ccagcccggc ggcaatcagg aaattgcggt tgtgcggcag tttccgtacg
    12781 aagagctcga acgtcgcttt ctcatgaacc ttgcccgcct gatagtaccc ggcagccatc
    12841 gtaagctgat acaggtccgt aagaaggcct tgcattctct tacgctactt ctttttctca
    12901 tccttcgccg cttgcgccgg tttgacgtcg cccgcggcct ggcggagttc ctcgacggtc
    12961 gcggcatcta cttcgtacaa cgtcggttcg ttcgcgcgtt tcgcgatatg gctttcgccc
    13021 gctttggaga tctcaaccgt ttcggtttgc ttgccttcgt tcgaggtaac cgtgatttcg
    13081 aataccggtg ccgtgaagcc ctgctgcaca aactttacag ccgacagatc gcggattttg
    13141 tcgatgaggt tctgcacgct ggtggaatcg atctcggccg ccccggacat ccatttgtcg
    13201 cccgatttct caaacaccat cgtcttatca ccatccttca cttgcacctt ggaaggctca
    13261 ttccagccga aatcgaacag ttttttgtta cggaaccctt ccagatcctt atcaacagcc
    13321 tgacccaaat ccgcgttgat cttgtgcgtg ccctccagca cgctgctcct ggcgaaatag
    13381 tcctctttgt tcttgcggac ctccatctgc tccgttccgg cggcatcggt aacccgcact
    13441 acagctacct gcgcgccacc cgcaaatgag gccgcggttt tcctgtcgtc ctccgccgaa
    13501 agggaggtgt ccattttcgc atcgcgcagc tttcggacca gttcctcgat ttcgccgcca
    13561 tccgcccgca tgggactcgg cctgataatc gtccattcgt tcttgttgtt cttcccgaac
    13621 tcgaccgact gtcctttgcg aaccaattcg acccgcgaca acttgtcctg atcaaaccgg
    13681 agcaggcgtt tgtcccgcag atcggccgcc gtcttatcga gagaactctt gttccagctt
    13741 gcgatggtga agattcgcgg atcgttctcc aatttcacgt aagcagcgct tcccgtaggc
    13801 gtctcgtcgc caacgagaac ggttcggcta tctccgccct tcttggtgat cacgacgttc
    13861 atggttggcg catccagtcc gaacggtccc agatcggaag ccttttcctc caccagcttg
    13921 tccgaactca gggacgatac cgtcgaaagc agagaccgga cagcgtcctc gtccgcggcc
    13981 agcggcttcg gagaagtgat ttcccatttc cccgcgtccg tcttccggat gatggtttcc
    14041 gggccatcct tccgcttgat ctcaacctgc tggatctgtt cttccggaat ttccagaatc
    14101 ttcgggcctg cgtccgccgc cggttgggca gccttcttct cttcgacttt attggaccag
    14161 tagactccgc cggccaaaat cgccagcacc accgcaccaa tcaataaacc acgagaaccc
    14221 atcggctatc tcctgcgcca ccagacgctc aggccggccg cgataatgag gagcggaaga
    14281 aataccacgc tcgaataaaa aaccactcgc atctggctgc cggtaagcgt caaccgccgg
    14341 tcttcgggct ccttcggccg aatggagatc aggtcttcat cggccgagag ccagttgatc
    14401 atgttcaacg ccaggtcccg gttcccgttg aaccggagaa ttccgttggt gacccagccc
    14461 gacgaacccg tgacgacgaa ccgccccttc tgattctctt tgcctgtgtc gtaggttccg
    14521 gcaacgccca gcgtcagcgg acccttcttg tctttcgcct cattgggctg tatctccgcg
    14581 gaactcagat tggttgtggc aaagctgttg ttcgaagtcg agaagagctt atctacggac
    14641 gttttctccg ccgtcgttgt gtcgagcgat cgggacagcg ggaacgcggt cgctacttct
    14701 ttcatctcgc gcacgatcgc gtgggattcg taattgacaa ccagcggcac cgctgcgctg
    14761 aggccgaaaa tctggccgaa tccgctggta tccagcacca ggtccttttc cgctttgaca
    14821 ccccagcttt ccagcagttt tacaagcccc ggattgtcgc tgatgttttc gcggcccatc
    14881 cggagcggcg ggtcaatcat gaacagcgcc gatcctccac cttcgacgta ggcacgcagc
    14941 gcgttcaccg ccggctctac ataatcgaaa cgaggcccac cgaccacgac gatcgtgcaa
    15001 tctgacggga cttccggctt ttcgagcagc gagatcgtgc gggtttgata attgcttttc
    15061 tccagcgcat ctttgaactg cgaatagccg tcgcgattcg tctcactgat gctgtgctca
    15121 ccactcccct gcacaaaaca gacgctgcgt gttcctgttt tcaacgcccg gatgagcgcg
    15181 cccgttatct cttcttcggt cagactgcgg gcctcctcct tcctgtcacc cgccttgaca
    15241 aaaacggtcc cctccgtact cacaccctcg gcgcgtgctc tctggggatt cttcagaaca
    15301 tccacgtatt ccacggacag cttcggcgag agattgtcgt accgcccaag caggtcctgc
    15361 gcgctcgtga accggctcga cacgtcgtag tagctgacag taacatcctg cttcaggttc
    15421 ttgacaacct tttccgtctg atcggaaaga ctgaaccgct tgttcgccgt cgagtcatag
    15481 gacttgttat gccggttggc cagaaaattg accagcgcca gcactccaat gatgatgacg
    15541 atatacagcg tcgcgtatgc cgtgtagcgt gtctgcctgg tcttgatcca atctgttttc
    15601 atttacgacc tccaccggag ggactcgagc gagcgggccg ttagaaacag cccgaagaag
    15661 atcattgaga gatagaagat gatgtcttta ctatccagaa cacccttgga aaatggctca
    15721 aaatgcgcca ccaccgagag atacgacatc accttggccc acgtcgtggt ctcgtagctc
    15781 acccagtcga atacccacaa cagcaaacaa acggcaaacg tagcggtgcc tgcgatgatc
    15841 tggttttttg tggtggtgga aatgaaggtt ccgatggcga gcaaagcgcc gcccagcagc
    15901 agcagtccca ggtagccgac cagaatcggc ttccagtccg gtttgccgta cagaaagagg
    15961 atggccaggt tcagagccga aatcaacaat atgcccgcat ataggatcac ggcggccagc
    16021 cacttgccga caatcacctc gatgtctttc accggcgatg tcaccagcag ttcgatggtg
    16081 ccggttctct tctcttcggc gaacagccgc atggtgatca tcggaatcag gaacagcccg
    16141 atcacgctca cgttggaaag aaacccgcgc acaacccact cgttcacatc catgggaaag
    16201 ctctgcccca tcatctggga ctgaatgccg cgttgcacga aaacggcggt aatgacgtaa
    16261 aagaagtaac ccgagatcgc ggcgaagaat gccatcagcc cgtaggcgat cggcgaggcg
    16321 aagtagctct tcaactcttt tctacaaatg atccagatat ttttcacttg gcacccactg
    16381 gtgattgagt cgcatcggac gacgtcaatt gcaggaagat ctcttccagg ctgagagcca
    16441 ccggccggag ttcgttcagg ttccagcccg cctcaaccac cgaacgcgcc aggtctcccc
    16501 tgacggtccg gccctgcaag ctctccactt caaacgtata ccggccatcc cggctgtccc
    16561 gcaaaagaac ccggctgacg cccggcacct gttccaaacg ctgttgtatc tccgcgctac
    16621 cggtcgaacc ttgcggcggt tcgatttcca ggccgacggc ttccgaaccc cgcaagcggt
    16681 tcgtcaggtt gtgaacggaa tcggttgcca ccaccttgcc cttgctgata atgatgacct
    16741 tttcgcaggt ctgctccact tcgggcagaa tgtgcgtgct cagaatgatg gtgtgcgctc
    16801 cagacaggct cttgatgagc tggcgtgttt cgagaatctg cttgggatcg agccccgccg
    16861 tcggctcgtc caggatcaga acgtcgggat tgtgaatgat ggcctgggcc agccccacgc
    16921 gctgccggta tccctttgag agtttgctga tcagtttcgt gcgaacgtcc gtgacggagc
    16981 agcgctcgga cacctcatcc acccgcgcgg caatgtcgct cttcggaatc cccttcagcc
    17041 gtccgacgaa gtggagatac tcgaccacct ccatttccgg atagagggga ggtgtttccg
    17101 gcagatagcc aatgcgcttc ttcacctcca gcggctgcgt aagaacatcg aaacctgcca
    17161 cgctcgctcc ccccgctgta ggtgggagaa agcacgtcag aatacgcatg gtggtggttt
    17221 ttcctgcacc gttgggtccg agaaacccga cgatctcgcc cttattcacc tcgaatgaga
    17281 tctggtctac ggcaaccgta cgagcgtatc gcttcgagag tccttccact ttgatcataa
    17341 atatcctgga atgagaagac gaatcttatc taacacaagg gtacacaatg gcgcccacta
    17401 ccacggaccc agcagattga aactacatca taagaatctg ccgaaactgc tgtcaaactc
    17461 cggcggcgct ggcgcgatag aacgtcaagc ggccacaata cgctctttcc cggcgtccca
    17521 tttcatgtac ttgcgtttac gataggactg caccgacatc tc
//
LOCUS       DASTMK010000358         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_337271, whole genome shotgun sequence.
ACCESSION   DASTMK010000358 DASTMK010000000
VERSION     DASTMK010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337271"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..3328
                     /locus_tag="VFQ79_15800"
     CDS             137..3328
                     /locus_tag="VFQ79_15800"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0141187.1"
                     /translation="MKRTIQLLLLPAIVAAGGFAQSLNTGTFSGSVYDASGAVIPGAT
                     IRITSENTAFQRETTADAEGNYQLLQVPTGTYTIEFEREGFQKSVQKGVAISAGQNLR
                     VNGNLTVGSVTETVEVGARVAQVDTTSANVGATVYGSQVQELALTTRSFTQLITLQPG
                     VASNQAQQPGFGSNTSVPFSFNGGQQSSNNWLLDGGRNVDTYNGNNLTMVNLDAIAEV
                     RIERNAYSAEYGRNSGAQVNVITRSGTNQFHGSAFEFFRNDKLDSRNFFATAKPKNRY
                     NNFGWTLGGPIKQDKVFFFLSNEYRRILQTTGTRTAAVPTARQLAGDFSGGRVINDPT
                     TGQPFPNNRIPADRLDPNALALIRGYYAEPTPGFQQGALNFTSSEPDGTKYRSGLGRV
                     DYNYSPTMTFFGRYNIDSTRLDSPYGLFAANSMPGVAASEQAHIMYTANGSMNWTIRP
                     TLLNQFTMSWYHGSMAISTVPFAARDRVPDFGVPRYFDTDTDSAGFIPAISMSAPYAG
                     ISILWPQNISHYTWELMDNMSWIKGRHIFKFGGSLSRENKTQNSSNINNNGIFTFNAS
                     YSGDALADLLLGRAFSYAETSHHRMGSAKFNGVGLYFQDQYRATNRLTLTAGLRWEFF
                     QPEYDPDGLISYFDPNAFDRSNAAVVLPNNGEIQPGTENFGNGIRVACETSEFGCAVT
                     NTRYKTFAPRVGFSYALTQDQMTVVRGGYGIFHDRWSQFVSGSRNNYPFNQSISIYNT
                     LFSNPAQGTRRIFPSNVTNMMSPWEVPYLQKWSLDLQRQLFGDLVVTVGYVGSKGTHL
                     IRTLDINQPQPSLAIATGQVNPNAVRPYPGFAAITSYSTSADSIYHSLQASGVRRFGG
                     GVSIQASYTWSKSLDNVITPFNIYWDGALNRGISSFDRRHMLIASYIWEIPFAKSTTG
                     WKRKVLDGWQISGISSFQSGNPLTIGITGDRAGTGAGGQRPNLIAPVDRPRELSQWFS
                     TSSFALPAQGTFGNAGRSLVTGPGINNWDVSFSKRTDLTESVSLQFRAEFFNLFNHAQ
                     WSGVGTTFGTGTFGQVTSARDPRIGQLGLRLLF"
     gene            3493..4164
                     /gene="eda"
                     /locus_tag="VFQ79_15805"
     CDS             3493..4164
                     /gene="eda"
                     /locus_tag="VFQ79_15805"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU0141188.1"
                     /translation="MKKETVLSFIRDVGIVPVVRTNSAESAIRSIEAIYRGGIRAAEI
                     TMTVPGAIKALEKVADQFGDKIVLGAGTVLDPETARACMLAGAEFFVTPALRVSTIEM
                     AKRYSKVICPGALTPTEVMTAWDAGADIVKIFPCGNVGGPKYIKALKAPFPHIEMIPT
                     GGVNLETAGEFLKAGACAVAVGGELVDAKTIAEGKFEVFEERAKKYLEVIARTRSEMK
                     AAATA"
     gene            4202..4372
                     /locus_tag="VFQ79_15810"
     CDS             4202..4372
                     /locus_tag="VFQ79_15810"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEU0141189.1"
                     /translation="MAISKDLLEILVCPLCRADVVLLENERGLKCVQCKRVYPIRDDI
                     PVMLIDEATIEE"
     gene            4369..>4804
                     /locus_tag="VFQ79_15815"
     CDS             4369..>4804
                     /locus_tag="VFQ79_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HEU0141190.1"
                     /translation="MTSSRTGTVLDRVPPGARVAIIRLRSLGDCILTTPAIRLLKQAR
                     ADLETGIVVEERFAAVFEDNPDVSCILRPRAADVAAWRPFLSVNLHGGTRSLALTLAS
                     NARIRAGFRHFRGACLYSTGIPTAQEILGITRKVHTAEHLASA"
BASE COUNT         1015 a         1473 c         1386 g          930 t
ORIGIN      
        1 cgttcggttc cagattaacg aacgtgaatg cttgtgcctt cctcttgacc tccggcctcc
       61 ggatctgata attgggtaac gactggatcc atcgttccgc acggacggtt ggtctcaatg
      121 cttcggggca aaactcatga aacgaaccat acagctacta ttgctgccgg cgatcgttgc
      181 cgccggcggc ttcgcacaat ccctcaatac gggaaccttc tcaggctccg tatacgacgc
      241 gagcggcgct gtcattcccg gcgccacaat tcgaatcacc agcgagaata ccgcttttca
      301 gcgtgaaacc acagcggacg cggaaggcaa ctaccaactg ctgcaagttc ccaccggcac
      361 gtacaccatt gaattcgaac gtgaaggatt ccagaaatcc gtacagaagg gcgtcgcgat
      421 cagcgccgga cagaacctgc gcgtgaacgg caacctcacc gtgggctctg tgaccgagac
      481 ggtggaggta ggggcacgcg tcgcgcaggt agacaccacg tcggcgaacg tcggcgcgac
      541 cgtctacggc tcgcaggtgc aggagctggc actcacgacg cgcagcttta cacaactgat
      601 cacgctgcaa cccggcgtcg catcgaacca ggcgcaacag ccggggttcg gatcgaacac
      661 cagcgtgccg ttttccttca acggaggcca gcaaagctcc aataactggc ttctcgatgg
      721 aggccgcaat gtcgatacgt ataacggcaa caacctgacg atggtcaatc tggatgccat
      781 tgcggaagtg cgcatcgaac gcaacgccta ttcggcggag tacgggcgca attccggagc
      841 ccaggtgaat gtgatcacac gctcgggcac gaaccagttt cacggctctg cgttcgagtt
      901 cttccgcaac gacaaactgg actccaggaa cttcttcgct accgcgaaac ccaaaaaccg
      961 ctataacaat ttcggctgga cgttgggcgg ccccatcaag caggacaagg tattcttctt
     1021 cctctccaac gaataccggc gaatcctaca gactacggga acccgtaccg ccgccgttcc
     1081 tacggcccgg caactcgcgg gggatttctc gggcggacgc gtcatcaacg accccacgac
     1141 ggggcagccg ttcccgaaca accggattcc cgcggaccgg ctggatccga acgcgctcgc
     1201 gttgatccgc ggctactatg cggagccgac gcccggtttt cagcaagggg cgctgaattt
     1261 cacctcgtcc gagccagatg gcaccaagta ccggtcgggg ctgggacgtg tcgattacaa
     1321 ctacagcccg accatgacgt tcttcggtcg ctacaacatc gatagtacgc gtctggacag
     1381 cccttatggt ctcttcgccg ccaactccat gccgggcgtt gccgcttccg agcaggccca
     1441 tatcatgtac acggccaacg gctccatgaa ctggaccatt cgcccgacgc tgctcaacca
     1501 gttcaccatg tcctggtatc acggctcgat ggcgatctcc acggtaccgt tcgccgcccg
     1561 cgatcgcgtt ccggactttg gcgtccccag gtacttcgac accgatacgg actcggcagg
     1621 ttttattccg gctatcagca tgtccgcgcc ttatgccgga atcagtatcc tctggccgca
     1681 gaacatctcg cactatacgt gggagttaat ggacaacatg agctggatca agggccgcca
     1741 tatcttcaaa ttcggaggct ctctgagcag ggagaacaag acgcagaatt ccagcaacat
     1801 caacaacaac ggaatattca ccttcaacgc ctcctacagc ggggatgcct tggccgatct
     1861 tctgctgggc cgggcgttct cctacgcgga aacttcgcat caccggatgg gcagcgcaaa
     1921 gttcaatggc gtagggctgt actttcagga tcagtaccgt gcgacaaacc gcctgacgct
     1981 caccgcggga ttacggtggg agttcttcca acccgagtac gatccggacg ggctgatatc
     2041 gtacttcgat ccgaatgcct tcgatcgctc caatgccgct gtggtgctgc cgaacaacgg
     2101 ggaaattcag ccaggcaccg agaacttcgg caacggcatc cgggtagcgt gcgagacctc
     2161 ggagttcggt tgtgcggtta ccaacacccg ctacaaaact tttgcgccgc gagtgggctt
     2221 ctcttacgcg ctgacgcagg accagatgac ggtggtgcgc ggcggatatg ggatcttcca
     2281 cgaccggtgg tcgcagttcg tctcgggttc gcgcaacaac tatccgttca atcagagcat
     2341 ttcgatttac aacacgctgt tctccaatcc ggctcagggc acgcggcgca ttttcccgtc
     2401 caacgtgacg aacatgatgt cgccctggga agtgccgtat ctgcagaaat ggtcgttaga
     2461 cctgcagcgc caactgtttg gcgacctggt ggtcacggtg gggtatgtcg gatcgaaagg
     2521 gacgcacctg atccgtacac tggatatcaa ccagccgcaa cccagcctgg ccattgcgac
     2581 cggccaggtc aacccgaacg cagtccggcc ctatccggga ttcgccgcca tcacttctta
     2641 ttcgacgtcg gccgactcca tataccactc gctgcaggct tccggcgtgc gccgtttcgg
     2701 cgggggcgtg tcgatccagg cctcctatac ctggagcaag tcgcttgaca acgtgatcac
     2761 gccgttcaac atctactggg acggcgcttt gaaccggggc atctcgagct tcgatcgccg
     2821 ccacatgctg atcgccagct acatctggga gattccgttt gcaaagtcca ctaccggatg
     2881 gaagaggaag gtcctggacg gctggcagat ttccggtatc tccagcttcc aatcgggcaa
     2941 cccgctcacc atcggcatta ccggcgaccg ggcaggcacc ggagccggtg ggcagcgtcc
     3001 gaacctgatc gcgccggtcg acaggccgcg cgaactcagc cagtggttca gcacgtcgtc
     3061 gttcgccctg ccggctcagg gcacgttcgg taacgccgga cggtcgctcg tcaccggccc
     3121 gggcatcaac aactgggacg tgtcgttcag caagaggacc gacttgacgg aatcggtctc
     3181 gctgcagttc cgtgccgaat tcttcaacct cttcaaccac gcacagtgga gtggagtagg
     3241 gaccacattc ggaaccggaa cgttcggaca ggttaccagc gcccgcgacc cgcgcatcgg
     3301 ccaactgggg ctgcgcctgc tgttctgacc ggctcttgcg ctaagggacc gtcagttcgc
     3361 caagaccgct cgctaacgcg cgcggcttcg taatcgcttc tgagccgcga acgtaagtga
     3421 gtggctggca ggggtccggt tcggcagttt gccccggcgt gtaaattgcc agacttggta
     3481 agatcaagag aaatgaagaa agagactgta ctctccttta tcagggacgt gggtatcgta
     3541 cccgtggtgc ggaccaacag cgccgaaagc gccatccggt cgatcgaagc catctaccgc
     3601 ggcggaatac gcgccgctga gattaccatg accgtccccg gcgcgatcaa agcgctcgaa
     3661 aaggtggccg accagttcgg cgataagatc gtgctcgggg ccggtacagt gctcgacccg
     3721 gaaaccgcgc gggcgtgcat gctggccggc gcggaatttt tcgttactcc ggcgctgcgc
     3781 gtctccacca tcgagatggc aaagcgctat tccaaggtga tttgcccggg cgcgctcact
     3841 cccaccgagg tcatgacggc atgggacgcc ggcgcggata ttgtgaaaat tttcccgtgc
     3901 ggaaacgtcg gcggccccaa gtacatcaaa gcactgaagg cgcctttccc gcatatcgag
     3961 atgattccga cgggcggcgt gaacctggaa accgcaggcg agttcctgaa ggccggcgcg
     4021 tgtgccgtag cggtcggcgg agaactggtg gacgcaaaga ctattgcaga aggcaaattc
     4081 gaggtgtttg aggagagggc caaaaagtac ctcgaagtca tcgccaggac aagatcggag
     4141 atgaaggccg cggcgacggc gtaaatgcgg ccggccggct tttgttaaac tggtgccgtt
     4201 tatggcgatc agcaaggatt tactagagat actggtttgc ccgttgtgca gagccgacgt
     4261 cgtgctgctc gaaaacgagc gcggcctgaa atgcgtgcaa tgcaaacggg tctatcccat
     4321 tcgcgacgat atccccgtca tgctgattga cgaggccaca atcgaagagt gacttcgtca
     4381 cgcaccggaa ccgttctgga ccgggtgcct ccaggtgccc gggtggctat cattcgtttg
     4441 cgttcgttag gagattgtat cctcacgacc ccggcgatac gcctgctgaa acaagcacga
     4501 gcggacctcg aaaccggtat tgtggtggaa gaacgtttcg ctgcggtgtt cgaagacaac
     4561 ccggatgtat cctgcattct ccgtccccgc gctgccgatg tggcggcctg gagacccttc
     4621 cttagcgtga atctccacgg aggaacgcgc agcctggcat tgaccctggc atcgaacgcg
     4681 cgcatccgcg caggattccg gcatttccgc ggcgcctgcc tgtatagcac gggcattccc
     4741 accgcgcagg aaattctcgg aatcacacga aaagttcaca ccgccgaaca cctggcttcc
     4801 gcca
//
LOCUS       DASTMK010000359         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_19274, whole genome shotgun sequence.
ACCESSION   DASTMK010000359 DASTMK010000000
VERSION     DASTMK010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19274"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFQ79_15820"
     CDS             <1..394
                     /locus_tag="VFQ79_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141191.1"
                     /translation="RQKPLGFERRRAPLFETHAPPSTLDRCLLEASFSIRLIPLYYAS
                     VSNLAILAERASISRTTLNRIEKGDPNAALGNYATVLFALGMVDRLADIADARHDTVG
                     LELEEEHLPQRIRLSRRQKPGKPEKTGA"
     gene            588..1139
                     /locus_tag="VFQ79_15825"
     CDS             588..1139
                     /locus_tag="VFQ79_15825"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEU0141192.1"
                     /translation="MRRAERRRAEREGQAPRTLREIDHLLMADDEARAGALRFTEREG
                     GPFLAEHGPAKIPPLIELPRLLSAAEHVLTGTGSDEDLRLLLAPGSSLGGARPKASVR
                     DRDGHLAIAKFPNKGDEWNTVVWEAVALTLAERASIPAPHWRLERSAAVFRPVSAMWT
                     LSGVRVGKRGLGERPSRNGAVSM"
     gene            1180..2167
                     /locus_tag="VFQ79_15830"
                     /pseudo
     CDS             1180..2167
                     /locus_tag="VFQ79_15830"
                     /inference="COORDINATES: protein motif:HMM:NF042573.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
BASE COUNT          445 a          633 c          762 g          428 t
ORIGIN      
        1 gcgccaaaaa cctctgggat ttgagcgacg gcgcgcgccg ctctttgaaa cgcatgcgcc
       61 gccgtccacg cttgatcgct gccttctgga agcaagcttc tctattcgat tgattccgct
      121 atattatgcg agcgtcagta atttagctat tctggcagaa cgggcttcga tcagccggac
      181 aactttaaac cggatagaga agggcgatcc taatgccgct ctcggcaatt acgcgaccgt
      241 tctctttgcg ttgggaatgg ttgaccggct ggccgacata gccgatgcgc gtcatgacac
      301 ggtgggcctc gagcttgagg aagagcatct tcctcagcgc atccgtctct cgcgccggca
      361 gaagcccggt aaaccggaaa agacgggtgc gtgacggaca gggaagtttt cgttcacatg
      421 gacctgcagg gcacgcccta tttggtgggg cggctgtgga cgcgcatccg caaggaccgg
      481 gagagcgcca cgtttgaata cgaccggggc catttcacac gccatcggat aagccgctgt
      541 tcggagcggt tggcgattcc gctcccgacc agtggagacg cgttctcatg cgccgcgccg
      601 aacgcaggcg tgcggaacgc gaaggtcagg cgccgcgtac cctccgcgaa atcgaccacc
      661 tgctcatggc ggatgacgag gcacgcgcgg gcgcgctccg attcacggaa agggaaggag
      721 ggccgtttct cgccgagcac ggccctgcga agatcccgcc gctgatcgag ttgcctcggt
      781 tgctatccgc cgccgagcac gttctgaccg gtaccggcag tgacgaggat ctccgcctgc
      841 ttctcgcacc cggatcgtca ctcggcggag cccgtccgaa ggcatcagtc cgggaccgcg
      901 acggtcatct cgccatagcg aagtttccaa acaagggaga cgaatggaac acggtggtat
      961 gggaagccgt ggccctgact ctcgcggaaa gagcgagtat cccggcgcct cattggcggc
     1021 ttgagcgatc agcggcggta tttcgtcccg tgtccgcgat gtggacacta tcaggtgttc
     1081 gtgtgggcaa acgtggcttg ggagaaagac catcccgaaa cggcgcggta tcgatgtgag
     1141 gcgtgcgagg cactcctcga gaactggcag aaaacggaca tgctcgcacg gggcgagtgg
     1201 cgcgcaaccg cgtccggtga cggaaagacc cgcggctatc acatttccgc cctatacgct
     1261 cctgtaggct ggccgtcctg gggcgaactg gcagcggagt tcctcgaagc acgtaagacc
     1321 cgcgaaacac ttcaggtctt ctgcaacacc gtctggggcg aagtatggcg cgacgaaacg
     1381 gcgttaccgc tggatgcgga cgcgctatac tcgcgccgtg aaccgttcgg ggcggaagtc
     1441 ccgatgggcg ggtgtctgct gactgccggg gcggacgttc aggcggatcg tatcgaggtc
     1501 gaaatcgtcc gatggggagg aaacctggag tgtgggttat cacgtgctgt acggcgacac
     1561 cgggcaaccg gaggtgtggt gtgaacttga ccggctattg cttcgtcagt ggaggcatga
     1621 aagcgggttc gatctgccga tcagcgcggc gtgcatagat gctggcttcg aaatggcgca
     1681 agtactggag ttctgccggc cgcggctgaa tcgcaaggtt tacgccgtga aaggcgcgtc
     1741 gggcttcggt aaaccgatct ggccgagacg tgcgtcgaag ggcgtgcata agagcgagtt
     1801 cttcatcgtg ggcagcgaca cggcaaaaga acgggtgtac ggcaaattgc gggtgaatct
     1861 gccgggcgcg ggacattgcc atttcccgct gaatcgggag tgcgattact tcgacatgct
     1921 gacggctgag cgcgtcaaga cgcgcgcggt tcacgggcgt gcagagaggt acttctacaa
     1981 gcctgacggc gtgcggaatg aggcgttgga ctgccgggct tatgcgacgg cggcgttgca
     2041 cgcgctctat gtgagcgggt tcaagttggc cgatcaagcg gcgcgcatga aagccatggt
     2101 ggcgggcaaa ccggacccgg cgcccgcgta tcaggtgtac cgcagcaagt ttctaggtgg
     2161 agcgtaagcc ataatgtttg ttgctcacgt aagaccaggc cgacgtacta cgtccagcgc
     2221 tggaagtgcc ggccgtcggg cgtcatgtgt ctgtttaccg tgagcaga
//
LOCUS       DASTMK010000360         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_49800, whole genome shotgun sequence.
ACCESSION   DASTMK010000360 DASTMK010000000
VERSION     DASTMK010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49800"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..844)
                     /locus_tag="VFQ79_15835"
     CDS             complement(122..844)
                     /locus_tag="VFQ79_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEU0141193.1"
                     /translation="MQGLGRAYLRFYAELNDFLPAERRFREFEYPFYVSGSVKDVIES
                     AGVPHTEVDLVLVNGDSKGFDYRVRNGDRISVYPVFEAFDIRSISRLRGEPLRAVRFV
                     LDTHLGKLARYLRMAGFDAHYRNDLSDSELVRISTGERRILLTRDVGLLKHSALTHAS
                     FVRETDPRRQFVEVLKRFDLRKPAAAFSRCLECNKPLERSGSSEFHRCPACGRIYWKG
                     SHYSRMQSFLAQMFEQVHQSSR"
     gene            complement(855..2057)
                     /locus_tag="VFQ79_15840"
     CDS             complement(855..2057)
                     /locus_tag="VFQ79_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141194.1"
                     /translation="MGAISALAFAVVVAGQPAFLRTACSSESSAIAELHPGDSVEIRF
                     ALSDASGTCYKVAVGGREGYVPASALSGMDAFERERKTASDVDLPRLMRAQVDAVRGG
                     LSLAAGEAAIAAIRLLDSGQPAEALRVLERIANGTNSDPQVFALAGVAAYRSDDVRRA
                     ADYWSRSLALRENTSIRQMHRKAERELAADRSAEKKYGTRFMLRYDSGAISSETARAM
                     VSVLEEEYSRIDAELGCQWSERITAIVQTRAAYLQTTEAAEWSGGQYDGRIRIALMEG
                     DTVGPGTRRVFAHELVHACLSSIGKWPAWLQEGLAQKLSGETLTHTARQRVQSYFRQG
                     RMPRLDRLGQDWSRLSPLHAAMAYGFSLAAVESLAETHPGMSYRTLVHSPHLLPQVTQ
                     ALNQRLAQ"
     gene            2154..3341
                     /locus_tag="VFQ79_15845"
     CDS             2154..3341
                     /locus_tag="VFQ79_15845"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0141195.1"
                     /translation="MNRNLHAMTRREILAASGLGVIAAGQSMGADRAAPASPVSIAKC
                     AGYDDDLARVLGRQFDQIGGIGRLVSGKTVAIKLNLTGGGRIGNYTPGQTHWVHPTLV
                     GVCCHLFGRAGAKRIRLLEGAARGDSLEDKMLNAGWDVEALRNAAPLVEFEDTNRLGS
                     GKRYARLKVAKPYIYPAFDLNPSYEDTDVFVSLSKLKHHEECGITLTIKNSFGIAPNS
                     VYGDDAGVDEPNERARRGRESILHYGRRQPPKSAPQEVDFTSDRFEGYRVPRISVDLI
                     AARPVDLAIIDGIESSIGGEGPWVKGVRYAKPEVLIAGRNPVCTDAVATAVMGYNPRA
                     VPGELPFRSTKETGEGEPRHPANPMLLAEAAGIGNAELARIEVLGVPIKQALYEFESR
                     RGG"
BASE COUNT          637 a         1034 c         1052 g          626 t
ORIGIN      
        1 ccggcatcgg aaccattatc gccatgatgg ttccttactc tgtggtgttt ctggtcttgt
       61 ggactgcact gctggtggtg tggatcgcgt tcggactgcc ggtcgggcca ggcgcgggtc
      121 tctacctgga agattgatgc acctgttcga acatctgggc cagaaaggat tgcatgcggc
      181 tgtaatgcga gcctttccaa tagatccgcc cgcacgcggg gcagcggtgg aactcgctcg
      241 agccggaccg ttcgagcggc ttgttgcatt cgagacaacg tgaaaatgcc gccgctggtt
      301 tgcgtagatc aaacctcttc agcacctcta caaactgccg gcgcggatcc gtttcacgga
      361 cgaaagatgc gtgcgtaagt gccgagtgtt tcagaaggcc aacgtcgcgg gtcagcagga
      421 tgcggcgctc tccggtggag attcgcacca gctcggaatc gcttagatcg ttccggtagt
      481 gcgcgtcaaa accggccatc cggaggtatc gcgccagctt gcccaagtga gtatccagaa
      541 cgaaacgaac ggctcgcagc ggctcgcccc gcaaacgcga aatggagcgg atgtcgaagg
      601 cttcaaatac cgggtagacg ctgatgcgat cgccgttccg cacacggtag tcgaatcctt
      661 tcgaatcgcc gttgaccagc accaggtcca cttccgtgtg agggacccct gccgattcga
      721 tgacgtcttt cacggagccg ctgacgtaaa acggatattc gaattcccgg aagcggcgct
      781 ccgcaggcag gaagtcgttc aattcggcat aaaagcggag ataggcacgc ccgagtccct
      841 gcaatgctcc gattttactg cgccaggcgc tggttcagcg cctgggtcac ctggggcaga
      901 agatgcgggc tgtgcaccag tgtgcggtag ctcatgccgg ggtgggtttc ggcaagcgat
      961 tccactgctg cgagggagaa gccgtaagcc atggcggcat gtaatgggct gagccttgac
     1021 cagtcctgtc ccagccggtc gaggcgcggc attcgaccct ggcggaaata gctttgaacg
     1081 cgctggcgtg ccgtatgcgt cagggtctca cccgaaagct tctgggccag cccttcctgc
     1141 aaccaggccg gccacttccc gatgctcgac aggcacgcat gtaccagctc gtgcgcgaac
     1201 acgcggcgcg ttccaggtcc cactgtgtcg ccttccatca aggcgatccg gatgcggccg
     1261 tcgtattggc ccccgctcca ctcggcggct tcggtcgtct gaagatacgc agcgcgcgtc
     1321 tgcacaatgg cagtgatgcg ctcgctccac tggcagccca gttcggcatc gatccgcgaa
     1381 tattcctctt ccaacacact caccatcgct cgcgccgttt cgctggaaat cgcgccgctg
     1441 tcataccgaa gcatgaagcg cgtgccgtat ttcttttcgg cgctgcggtc cgcggcgagc
     1501 tcgcgctccg ctttgcggtg catctgccgg atgctcgtgt tttcacgaag ggcgagcgag
     1561 cgcgaccagt agtccgccgc gcgtcgaaca tcgtcgctgc ggtaggcggc aactcccgcc
     1621 agcgcgaata cttgcggatc ggaattcgtc ccgttggcga tgcgttcgag cacccggagc
     1681 gcttcggccg gctgcccgga gtcaagaagg cgaatcgcgg caatcgcagc ttccccggct
     1741 gcgagggaca agccgccccg gaccgcgtcc acctgcgccc tcatgagccg cggcagatcc
     1801 acatccgatg cggtctttct ctcccgctcg aatgcatcca tgccggataa cgccgatgcc
     1861 ggaacatagc cctcgcggcc tcccacagcc accttgtagc aggtgcccga agcgtccgag
     1921 agagcgaagc ggatttcaac agagtcgccg gggtgcagct ccgcgatggc cgaagactca
     1981 ctcgaacacg ccgtccgcag gaacgccggc tgcccggcaa ccaccaccgc gaaggcaagc
     2041 gccgatatcg ctcccatgac ccgatactaa gatggaaagc ccgcgcgtgt cgctcgggga
     2101 aatacctgag aaactcttgt attccgcaga ccgcggtgaa ataatgcgct cttatgaacc
     2161 ggaacctgca tgcgatgacg cggcgtgaaa tactggccgc ctccggcctg ggcgtgatag
     2221 ccgccgggca aagcatgggt gcggacaggg ccgccccggc ctctcccgtt tccatcgcca
     2281 aatgcgccgg ctatgacgac gatctcgccc gggtgctcgg ccggcagttt gaccagattg
     2341 ggggaatcgg ccgccttgtc agcggtaaga ccgtcgccat caagttgaac ctgaccggcg
     2401 gcgggcgcat cggcaactat accccgggac agacgcactg ggtgcaccca acgctcgttg
     2461 gtgtttgctg ccatttattc gggcgcgccg gagcgaaacg catccggctg ctcgagggtg
     2521 cggcgcgcgg cgattctctc gaagacaaga tgctcaacgc gggctgggat gtggaagcgc
     2581 tgcggaacgc cgcgccactg gtggagttcg aggacacaaa ccggctcggc agcggcaagc
     2641 gctacgcgcg cctgaaggtg gccaaacctt atatttatcc cgccttcgat ctgaatcctt
     2701 cttatgagga tacagatgtg tttgtcagcc tcagcaagtt gaagcatcac gaggagtgcg
     2761 gcatcacgct cactatcaag aacagctttg gcatcgcgcc gaattccgtt tacggagacg
     2821 atgccggcgt tgacgagccg aacgaacggg cgcgccgcgg ccgggagtcg atcctgcatt
     2881 acggccggcg ccagcccccc aagagtgctc cgcaggaagt ggattttacc agcgatcggt
     2941 ttgagggata ccgcgtgccg cggatctccg tcgatctgat cgctgcaagg ccggtggacc
     3001 tcgctatcat tgacggcatc gagtcttcga ttggcggcga agggccgtgg gtgaagggcg
     3061 tccgttatgc gaagccggaa gtacttatcg ccggcaggaa cccggtgtgt accgacgccg
     3121 tggcgaccgc cgtgatggga tacaacccgc gcgccgtgcc gggcgagttg ccgtttcgca
     3181 gcacaaagga aacaggggaa ggcgagcctc gtcatccggc gaatccgatg ttgctcgccg
     3241 aggccgcggg catcggcaac gcggagctcg ccaggatcga agtacttgga gttcctatca
     3301 agcaggcgct ctacgagttc gaatcccggc gcgggggtta gtctgttcg
//
LOCUS       DASTMK010000361         5170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_369378, whole genome shotgun sequence.
ACCESSION   DASTMK010000361 DASTMK010000000
VERSION     DASTMK010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369378"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..269)
                     /locus_tag="VFQ79_15850"
     CDS             complement(123..269)
                     /locus_tag="VFQ79_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141196.1"
                     /translation="MSRFHAALAAYLAIFLLASFTLKGPFRLAIWILLGGLAFKTWLA
                     TKRS"
     gene            complement(273..1340)
                     /locus_tag="VFQ79_15855"
     CDS             complement(273..1340)
                     /locus_tag="VFQ79_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0141197.1"
                     /translation="MATRLQSIPADREELKLLPEVDRSSAWKSGMRAAGVSIVAHLVL
                     VATLLSLPPQTPRSVPEPDEALVARRYTPLVAPPFELTQREPNRGRISKEVDLEALTP
                     SPRIRVPALPPGRRAAQRQFSLPGETKETQRPSVVPAPPKIQAEESRVAQVAPPGGTR
                     LLPAPPPQPPQEEELKLAFERPGAGGSGPSTPNVPVVVPGRSVDEAVRSVARSGGVGA
                     GLIVGDIEGAGGIGEALSINPARGRSGSNIELLSDPMGVDFRPYLIKVLSAVRRNWFA
                     VIPESAKFGRRGKVIIQFAISREGGVPKLVIAVPSGADALDRAAVAGISASNPFPPLP
                     AEYRGDQVRLQLSFFYNMSRN"
     gene            complement(1382..2332)
                     /locus_tag="VFQ79_15860"
     CDS             complement(1382..2332)
                     /locus_tag="VFQ79_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141198.1"
                     /translation="MSKIRMAVVGAGEFGRNHIRVIAENPRAELTAIMDTDAGRASEL
                     AKQFHCTGTSELAEIAGLADAVVVAVPTTAHSETGVRLMEMGLHVLVEKPIADDLAGA
                     RELVRASERTGRILQVGHLERFNPAVTALTEAVTVPLFFEVHRMSVFTPRSLDIDVVL
                     DLMIHDLDIVLSLVGEMPEEIRAAGLSILSGKVDIANVRLAFPGGCVANLTASRVSTE
                     RVRKLRLFQPNQYISIDYTRQEAVAFSVSKDRQISFNKLPVTAGEPLQFEIESFLESI
                     QKGVRPRVSGEEALRALEVAVAILDKIEEHSKAVAKALAH"
     gene            complement(2393..3208)
                     /gene="lpxI"
                     /locus_tag="VFQ79_15865"
     CDS             complement(2393..3208)
                     /gene="lpxI"
                     /locus_tag="VFQ79_15865"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687860.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEU0141199.1"
                     /translation="MKYGLIAGNGRFPLLALEAARQMGDEVVVVAIREEASKEVEALA
                     ARCYWISLGELSRLIEILKSEGISEIVMAGQVKHASIFSSIRPDWRLFKLLASLGTRN
                     TDSLILGVAKVLEDEGIRLIDSTRVLKPLLAPEGNLTRRKPDSEEQADIAYGRRVANA
                     LAGFDVGQSVAISGRACVALEAMEGTDAMLERAATLVNGRPLRLIKVSRRRKHLLFDV
                     PVAGPGTIATMLRTGTTALAVDANRTLLLDKEEMLSRAEEAGLAVVGILPDEP"
     gene            complement(3205..3981)
                     /gene="lpxA"
                     /locus_tag="VFQ79_15870"
     CDS             complement(3205..3981)
                     /gene="lpxA"
                     /locus_tag="VFQ79_15870"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEU0141200.1"
                     /translation="MPVHPTAIVDTQSRIADTAEIGPFCIIGPEVEIGPGTRLMAHVY
                     VEGPAWIGQDNIFYPYSTVGVASQDLKYRGERAETRIGDRNKIREFVTIHRGTEGGGL
                     VTSIGADNLLMAYVHVAHDVSIGNHCILANAVTFGGHVRVEDWAVIGAFSGVHQFCRV
                     GRHAMIGGYSVITQDVLPFSMTVAPRENKVFGANRTGLERRGFSTETIEGLQTALRLL
                     TRSGLNTTQAMERISGEVPASPELDELMAFIRSSERGIVK"
     gene            complement(3989..4453)
                     /gene="fabZ"
                     /locus_tag="VFQ79_15875"
     CDS             complement(3989..4453)
                     /gene="fabZ"
                     /locus_tag="VFQ79_15875"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU0141201.1"
                     /translation="MAILDNEAIRRILPHRYPMLLVDRIIEIDGERIVGIKNVTANEP
                     FFSGHFPEFPVMPGVLIVEAMAQVAGILVLRQMENRDSKLVFLASIEEAKFRRPVLPG
                     DQLRIEMKVGKRKATVAKMYGQATVDGVVVAEAQVMCKLADKEPAAAAQATP"
     gene            4477..>5170
                     /locus_tag="VFQ79_15880"
     CDS             4477..>5170
                     /locus_tag="VFQ79_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0141202.1"
                     /translation="MKQILSHVQAKQKETVALIRRLVECESPTDDAGAVNRFVDLVAA
                     EVQGEADIRTYPGGTFGRHLRIEFRLPGRRKTKQLLLLGHSDTVWPLGTLARMPFRER
                     DGRLWGPGVLDMKSGIAFAIQGARALRELDIPVSRRVVLLLNADEEIGSDSSRPLTER
                     EARRSAAVLVLEPGTGLEGKLKTARKGIGGFKVSVAGRASHAGVDFAAGASAIVELAR
                     QIAIIAGFTDLDR"
BASE COUNT          980 a         1628 c         1525 g         1037 t
ORIGIN      
        1 ttccgtttca atctgaaaca gaatgtggcc cctccatcta ggctatcggc tgtccgataa
       61 ttcgtaaaca gaggttttgg tctggattgc ctgaaaaaga aggggaaaat accttagtat
      121 cttcaactgc gcttcgtagc aagccatgtt ttgaacgcca gaccgcccag aagaatccag
      181 atcgccaggc ggaacggccc cttcagcgtg aaggaagcga gtagaaagat ggcgagatac
      241 gctgccagcg cggcgtgaaa ccgtgacatc cgtcagttcc tgctcatgtt atagaaaaaa
      301 gatagttgca ggcggacctg atcgccgcgg tattcggcgg gaagcggcgg aaaaggattg
      361 gacgcgctga tacccgccac cgctgcacgg tccaacgcgt cggctcccga aggcactgcg
      421 attaccagct ttgggacccc gccttcccgg ctgatggcaa actggatgat caccttgccc
      481 ctgcgtccga atttggcgct ctcgggaatg acggcgaacc agttgcggcg tacggcggag
      541 agcactttga tcaggtaagg ccggaaatcc acgcccatcg ggtcgctcag cagttcaatg
      601 ttgcttccgc tacggccgcg ggcgggattt atgctcaggg cctcgccgat tccacctgcc
      661 ccctcgatgt caccgacgat gagtcccgcg ccgactcccc cgctccgcgc cacacttcga
      721 acggcctcat ccacggaacg ccccggaacc acgacaggaa cattcggggt gctcggtccg
      781 gaaccgcccg ctccgggcct ttcaaatgcg agtttgagtt cttcttcctg gggaggctga
      841 ggcggtggcg cgggcagtaa tcgggtcccg cccggaggtg ccacttgtgc cactctggat
      901 tcctcggctt ggattttggg tggtgcggga accaccgacg ggcgctgtgt ctcctttgtc
      961 tcgcccggta acgagaattg gcgctgcgca gcccggcggc cgggcggcaa tgctggaacc
     1021 cgtattcggg gactcggtgt cagtgcttcc aaatccactt ctttgctgat tcttccacgg
     1081 ttcggctcgc gctgcgtcaa ttcgaaaggc ggtgctacca gcggtgtgta ccggcgggcg
     1141 accagggcct cgtcgggctc aggaacactg cgcggagttt gcggcggaag tgaaagcagg
     1201 gtggcgacca ggacgaggtg cgccacaatc gaaacacccg ccgcccgcat tccacttttc
     1261 caggcgctgg agcggtccac ttccggaaga agtttcaact cctcgcggtc agccggaata
     1321 gactgaagcc gagtagccat ggaaagactc agacgtaacg tggtgagcgg aggttgccgg
     1381 atcagtgtgc cagggctttc gcaaccgctt tcgaatgctc ctcaatttta tcaagaattg
     1441 ccacggctac ttccagcgcc cgcagcgctt cttctccgga aaccctcggt ctcacccctt
     1501 tttggatgct ttccaggaac gattcgatct cgaattgaag cggttctccc gcggtaaccg
     1561 gtaacttatt aaaagagatc tgccggtctt tggaaactga aaaggccacg gcttcctggc
     1621 gtgtgtaatc gatggaaatg tattggtttg gctgaaaaag gcgcaatttc cggacacgct
     1681 ccgtcgacac acgactggct gtcagattcg ccacgcatcc tcccggaaag gcgagacgaa
     1741 cattcgcgat atccactttt cccgacagaa tggatagccc agccgcacgg atctcctcgg
     1801 gcatctcccc gaccagagac agcacgatgt cgagatcgtg aatcatcagg tccagcacca
     1861 cgtcgatgtc caggctcctc ggcgtgaaca cgctcatgcg atgcacttcg aaaaacaggg
     1921 gaacggtgac ggcttccgtc agcgcggtca cggcggggtt aaaccgctcc aggtgcccta
     1981 cttgaaggat tctgccggtt cgttcgctgg cgcggaccag ttcgcgggcg ccggcaagat
     2041 cgtccgcgat gggtttctcc accagcacat gcaagcccat ttccatcagc cggacgcctg
     2101 tctcgctgtg cgcggttgtg ggaaccgcca ccaccaccgc atcggccaga cccgcaattt
     2161 ccgccaattc cgaggtgccg gtgcagtgga attgcttcgc caactcgctg gcgcgtccgg
     2221 catcggtatc catgatggcg gtcagttcgg cacgcgggtt ctcggcgatg acccggatat
     2281 gatttctgcc gaactccccg gcaccaacaa cggccatgcg gatcttgctc acttggttct
     2341 cctcgcctca aacgataaca cgcgattcct gccgctcagg gcagacccgc ccttacggtt
     2401 cgtccggaag aatgccaacc accgcgagac cggcctcctc ggcacggctg agcatttcct
     2461 ctttatccag cagcagcgtg cgattcgcgt ccaccgcgag agcggttgtc ccggtccgga
     2521 gcatggtagc gatcgttccg ggtcccgcga ccgggacgtc gaacaggaga tgcttccgcc
     2581 tgcgcgatac tttgatcagg cgcagggggc ggccgttcac cagcgtggcg gcacgttcca
     2641 gcatcgcgtc cgtgccttcc atcgcttcga gcgctacaca agcccgcccg gagatggcaa
     2701 cgctttgacc gacgtcgaaa ccggccagtg cgttcgccac acgacgtcca tacgcaatat
     2761 cggcctgttc ctcgctatcc ggcttccggc gggtgaggtt gccttccggc gccagcagcg
     2821 gcttcagtac ccgcgtggaa tcgatgagac ggatcccctc gtcttccagt accttcgcta
     2881 cgcccaggat gagggaatcg gtgtttcggg ttccgaggga ggcgagcagc ttgaacaacc
     2941 gccagtcggg gcgaatcgat gaaaaaatgc tggcgtgctt cacttgcccc gccatcacga
     3001 tctcgctgat tccttcgctc ttcaggattt cgatgagccg gctcagctca cccaaggaga
     3061 tccaataaca gcgcgcggcc agggcttcca cttctttcga ggcttcttcc cggattgcca
     3121 ccacgacaac ttcgtctccc atctgccgcg ccgcctccag cgcgagcagc gggaaccttc
     3181 cgttgccggc aatcagcccg tatttcattt cacgatgccg cgttcggagg agcggatgaa
     3241 cgccatcagt tcatccaact cggggctggc gggcacctcc ccggaaatgc gctccatcgc
     3301 ctgcgtggtg ttcaaaccgg agcgtgtcag cagccgcaag gcggtttgca gtccttcaat
     3361 ggtttcggtc gaaaaaccgc gccgctctag gccggtgcgg tttgcgccga acactttgtt
     3421 ctcgcgcggc gccacggtca tggagaacgg cagcacgtcc tgcgtaatca cgctatagcc
     3481 gccgatcatg gcgtggcggc ccacccggca aaactggtgg acgccggaaa aggctccgat
     3541 gaccgcccaa tcctccaccc ggacatgccc gccgaacgtt accgcattgg cgagaatgca
     3601 gtggttgccg attgatacat cgtgggccac atgcacgtag gccatcagca ggttgtccgc
     3661 accgatggat gtcaccagcc ccccgccttc cgtgccccgg tgtatggtga cgaattcgcg
     3721 gattttgttc cggtcgccga ttcgggtttc cgcgcgctcg ccgcgatatt tcaggtcctg
     3781 tgacgccacg cccacggtcg agtagggata gaagatatta tcctggccga tccatgccgg
     3841 cccctcgacg tagacatggg ccatcaggcg ggtaccgggc ccgatctcca cttccggccc
     3901 gatgatacag aagggcccga tctcggctgt atccgcaatg cgggactgtg tgtctacaat
     3961 cgcggttgga tgaaccggca tcggcgcgct aaggagttgc ctgtgcggcg gccgccggtt
     4021 ccttgtcggc gagtttgcac atcacctgtg cttcggcgac cactacgccg tccaccgtcg
     4081 cctggccgta catcttcgcc acagtcgcct tgcgctttcc aaccttcatc tcgatccgga
     4141 gctgatcgcc aggcagcacg ggcctgcgga atttcgcctc ttcaatggag gccaggaata
     4201 ccagcttact gtcgcggttt tccatctggc gcagcaccag aatacccgcc acctgcgcca
     4261 tcgcctcgac gatcagtacg cccggcatca caggaaattc cggaaagtgc ccactgaaaa
     4321 agggttcgtt cgcggtcacg tttttgatgc cgacgattcg ctctccgtcg atctcgatga
     4381 tccggtcgac aagcaacatg ggatagcggt gcggcaaaat ccgccggatc gcttcattat
     4441 ccaggatggc catttagact cgttattata acagcgatga aacagatcct ttcgcatgtg
     4501 caagcgaagc agaaagaaac cgtagcgctc atccggcgcc tggtggaatg cgaatcgccc
     4561 acggatgatg caggtgccgt caaccgcttc gtcgacctgg tcgccgccga agtccagggg
     4621 gaagccgata tccgcaccta ccccggtggc acgttcggcc gtcacctgcg catcgaattt
     4681 cgccttccgg gacgccgcaa gacaaagcag cttctgttgc tcggccactc cgacacggta
     4741 tggccgttgg gcactctcgc gcggatgcct ttccgggagc gtgacgggcg tctgtggggt
     4801 ccaggtgtgc tcgacatgaa atcgggtatc gcgtttgcca tccagggggc gcgtgcgctg
     4861 cgggagctcg atatccctgt ctcccggcgc gtcgtgctgc tgctcaatgc cgacgaagag
     4921 attggcagcg acagctcgcg gcctctcacg gagcgtgagg cgcgccgcag cgccgccgtg
     4981 ctggttctgg agccgggcac cggactcgaa gggaagctga agaccgcacg gaagggcatc
     5041 ggagggttta aggtaagcgt tgccggacgg gcatctcacg ccggcgtcga tttcgcggcg
     5101 ggtgccagcg cgattgtcga gctcgcccgc cagatcgcca tcatcgccgg gttcaccgat
     5161 ctcgatcgcg
//
LOCUS       DASTMK010000362        10302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_433784, whole genome shotgun sequence.
ACCESSION   DASTMK010000362 DASTMK010000000
VERSION     DASTMK010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10302
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433784"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..619
                     /locus_tag="VFQ79_15885"
     CDS             359..619
                     /locus_tag="VFQ79_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141203.1"
                     /translation="MNPLLPVLVYGSALALAFLLLFVFQARRWYWHALSVLAAVCLGL
                     VRFPENWYGPAFDLFIGFWFVFLLVWGIGAPFFRPERLPRHA"
     gene            757..1320
                     /locus_tag="VFQ79_15890"
     CDS             757..1320
                     /locus_tag="VFQ79_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0141204.1"
                     /translation="MLVRQMLEGSESAFTELYRRHQARVFRFALHMSGSRALAEEVVQ
                     EVFLAFLKDPARYVPERGNFGLYLLGMAKNYVLRMLHRTRDEDLEQADGPAAGDDPAD
                     GLARRQTIENIRKAVLSLPPGYREAVVLCDLEEMDYAEAAQVLDCAVGTVRSRLHRAR
                     AMLAERIRNKPELRDELSRFKVMRCLA"
     gene            1380..2075
                     /locus_tag="VFQ79_15895"
     CDS             1380..2075
                     /locus_tag="VFQ79_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141205.1"
                     /translation="MHAEMAEHAAACPECAAIGEEERLLSSALSALAASTSSSEAPEE
                     VAQALLTEFRAGRRTQRNIRRFSWTAAVAAGIGLVAVMGNWSVLKPRLQAPPAVAEKR
                     AGPVQTVPVQEAAPAPQPPAVERGAGARAASALTARVRSGRGVRRPVAPPARPEPLRE
                     YVTEFVPLRYGESLRLDDVAGVVRIAIPRSELARFGLPVAPEASGRRIQADVLLGVDG
                     VARAMRFVKTETY"
     gene            2101..3087
                     /locus_tag="VFQ79_15900"
     CDS             2101..3087
                     /locus_tag="VFQ79_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141206.1"
                     /translation="MEFSRVSMFIAGALAFCAASVLSGQETIVGQRIGYPPAQAAAVP
                     AEGTAGGVFQMALPPPPFGGEPGTFSLIAGQAGFGMVAVAGAPYSAQAVTETSRVLGD
                     GTRIQQKNTVAMYRDSEGRTRREQTLDGIGPWRSATGTVTVVFIHDPVAKEMYTLNPA
                     DKTATKSVIEERFDPAAGANRRRPGPPPDEMKATEVQVMRRVGGGPGGGAVLANRLFH
                     AGRAENSQTESLGQQNIEGVMAEGTRVTTTIEVGKIGNDRPITIVSEHWYSPDLKMIV
                     MSKRTDPLAGDTSYKVTNLVRAEQPRSLFEIPPDYTVKETGGFFRHTIEVKK"
     gene            complement(3136..3609)
                     /locus_tag="VFQ79_15905"
     CDS             complement(3136..3609)
                     /locus_tag="VFQ79_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEU0141207.1"
                     /translation="MQPAEHAARVIPSWDEYYLSICKIVATRSKDPNTQIGCVIVGPA
                     HEIRSTGYNSFPRGIRDDIPERVVRPAKYLWIEHAERNAICNAARAGTATEGCTIYVE
                     IMPCMDCARAIVQAGIVEVVVSDVRMRQYSSEYYNEHFGMVEVLFGEAGIRIRQA"
     gene            3896..4144
                     /locus_tag="VFQ79_15910"
     CDS             3896..4144
                     /locus_tag="VFQ79_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141208.1"
                     /translation="MKRIWTFLRDSWCYRMHPAPMWPVNGMYRCPECQREYPVPWEKG
                     VTRWDFQPAKEDVRESTVVSHPARSGFRIVKAFKRTAA"
     gene            complement(4197..5354)
                     /locus_tag="VFQ79_15915"
     CDS             complement(4197..5354)
                     /locus_tag="VFQ79_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141209.1"
                     /translation="MDNTAPTSEPRDSFPVYYVWQVAGKSIAVRLNLDLVDALLQEVM
                     SGFGAVPRRGAEVGGILLGSVKEDHGTVIVIDGFERVPCEYRSGPSFTLSEPELQRLR
                     TTIEAWKPSPGRKSHVVGYFRSHTRDGMGLSPEDIQIFNDYFPQPNAVALLVKPFATK
                     ASQAGFFFREGAAFRTESSYLEFPFRRRELGGSPTVRKERGAIPALAQPQHHQTVPAH
                     PPAPPEQPIPVMVTPALQPTEAPPANKSMPVPLTNSVWKFSAFLLLLLGIALGFVASR
                     FTSPETGEASAVDSYTLGVALERSGENVKITWDRSAPAIRRSLGGALVIRDGDSTRTV
                     NLTRASLQNGSVLYSHVSDHITFRLEVFFSNGAVLSHTEDYIAGERPVSNP"
     gene            5442..6275
                     /gene="rsmI"
                     /locus_tag="VFQ79_15920"
     CDS             5442..6275
                     /gene="rsmI"
                     /locus_tag="VFQ79_15920"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU0141210.1"
                     /translation="MAVSGTLFVVSTPIGNLDDLTFRALRVLKEAGVIACEDTRHTKK
                     LLDHYGIKRPLVSYHEHNENERAVELVGRLQRGESIALVSDAGTPLLSDPGYRIVARA
                     RQEGIPVVPLPGASALLAALAASGLPTDAFHFAGFLPPKPAARRKLLESLRDEHATLV
                     FYEAPHRILEALADVEAILGQRDVVVARELTKVHEEFLRGTAAEIGQLLAARGSVKGE
                     ITLLVARGRQASAGSGSVEEAVDALSAAGMPRMEAIKTVARERGLSKREVYRRAHEPE
                     H"
     gene            6309..9446
                     /locus_tag="VFQ79_15925"
     CDS             6309..9446
                     /locus_tag="VFQ79_15925"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HEU0141211.1"
                     /translation="MLRRIRCTALFLCVSTVLFSQTKPRLIVFTDIGGDPDDQQSMVR
                     LLSYANEFEIEGLIASAAGIPGELKENVVRPDLIRQMVAAYGHVRPNLERNKPGFPSA
                     GYLETRIKSGNPKRGVENIGAGKDTEGSNWIIKVVDRDDPRPVNVAIWGGATELAQAL
                     WRVRNDRGAAGVSRFAAKLRVHSISHQDNTGPWIVENFPELFFILNAKAAEDVLRVTP
                     SGPDRRLSSYRGMYLGGDESLTSREWIDTNVRHNHGPLGALYPPKTWTVPNPHSALKE
                     GDTPSWFYFLANGLSDPAHPEWGGWGGRFLPAGGGLYRDAVDTVDGVIDARATVWRWR
                     PAFQNDFAARMDWSVQPPDKANHNPIAVLNGNRTQGIVKVEVSSGAPVTLSAAGSADP
                     DGDRLSFRWFLYPEAGTYKGAVPIENAEEAVARIVAPGVQSPATVHVILKVRDGGTPA
                     LYAYRRAILTVQPKPKAAIAGERKLWHKITITFDGPSLSEEAMPNPFRDYRLNVMFTH
                     TSTGVRRAVPGYFAADGNAAESSASAGNKWRVNFSPDLPGEWRYQASFRRGTDVATSL
                     DVTEGEAAGFDGASGTFRVAAAGKPGNDFRSKGVLRYTGEHYLRHAETGERFLKGGAD
                     SPENFLAYADFDGTFDTDARVNEGMSARFSEPFIHHYEPHAADWTPGDPSWKNGKGKN
                     IVGALNYLASKGMNSVYFLTYNVDGGDGKDVWMWTSPGVRDRYDVSKLEQWEIVFSHM
                     TSKGLLLHVVTQETENDRKLGGGPGLNPVRRLYYRELVAHFSHHPAIVWNLGEENNTS
                     DADRKEIAAYLRALDPYAHPITVHTHNNKAPDFYNGVLGDSNFEAASIQGDMRNYYRD
                     AVVLRERSAKVGRKWAIFGDEQPSADVGVMPDESDPGHDIPRIHALWGNLLGGGSGVE
                     WYFGYKYPHMDLNCEDWRSRDRMWDQTRHALTFFHRHLPFWEMEPDNSLASSTSGARV
                     FAKRGEVYAVQLPGGTGDLSVPEGRYTVSWYNPRTGGDLVAGSVAYITGPGSQSLGSP
                     PSEPERDWIVLVKKR"
     gene            complement(9456..>10302)
                     /locus_tag="VFQ79_15930"
     CDS             complement(9456..>10302)
                     /locus_tag="VFQ79_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0141212.1"
                     /translation="DGGKSCWGSGFLPTVYQGVEFRSKGDPVLFLSNPPGVEMETRRR
                     SLDLLRDLNTDHLADIGDPEIATRINSYEMAYRMQSSVPELAEISKEPQHIHEMYGTE
                     PGNASFANNCLLARRLVERGVRFVQLYHRGWDTHGASNNEDIVNKLAHLCRETDQGAS
                     ALVKDLKQRGLLDSTLVVWGGEFGRTPMNEARSGSKFLGRDHHPRAFTMWMAGGGIKP
                     GITVGATDDVGYNIAEDAISVHDLHATILHLLGIEHTKLTYRFQGREFRLTDVEGLVV
                     KKLLA"
BASE COUNT         2052 a         2936 c         3208 g         2106 t
ORIGIN      
        1 ctcacccagg aacttcggaa tgccggctac accgtccaga tcacctcgcc ggacgccaaa
       61 ggggcacagc cgtgattttt ccggagtaac tttcgagaga gcggtagacc cgggttcgca
      121 aagcatggaa tgagctggcg agcctgtggc ggtcttcctg gggaatgtac cttgaagtcc
      181 gccaaccgct cgctgacgct cacggctcag ctacaagtcg cccctaccat agtaataagg
      241 tacagaaatc ttaatattcg cctataatgg taatggagac gctggctgcc caccgaagat
      301 gcgtcggtgt cctccggcca caaggttcag gccgccgtgc gcccgactgg aggtagctat
      361 gaaccctcta ttgcccgtgc tggtttacgg ctccgccctt gcattggcat tcctgcttct
      421 tttcgttttt caagcacgcc gttggtactg gcatgcgttg agcgtcctcg ccgccgtctg
      481 ccttggtctc gtccgtttcc ccgagaattg gtacggcccg gctttcgacc tgttcattgg
      541 cttctggttt gtctttctcc tggtatgggg tatcggcgcg ccgtttttcc gtccggagcg
      601 cctgccccgg catgcgtgag cgcccacggc tttgacgagc cacggccggt tctgaaaaat
      661 cgggaacttt catcgcctct tttgtgaata cacaatagag ggggcggtgg tattcgccct
      721 tttccggcgc gaatggacag tcgtgaacaa gatccgatgc ttgtcaggca aatgctggaa
      781 ggctcggaat cggcgttcac ggaactgtac cggcgtcatc aggcgagggt gttccgcttt
      841 gcgcttcata tgtccggttc acgggcactc gccgaagagg tcgtgcagga agtgtttctt
      901 gccttcctga aagatccggc gcggtatgtg ccggagcgtg gcaacttcgg tttgtacctg
      961 cttggcatgg cgaagaatta cgtgcttcgg atgcttcatc gcacacgcga tgaggacctc
     1021 gaacaggcgg acggcccggc cgccggggac gatccggctg acggtctggc acggcggcag
     1081 accatcgaaa acatacgcaa ggccgtgctg tcgttgccgc ccggatatag ggaagcggtt
     1141 gtgctgtgcg acctcgagga gatggattat gcggaggcgg cgcaggtact cgactgcgcc
     1201 gtgggcacgg ttcgttcccg ccttcatcgc gcaagggcga tgctcgcgga acgaatccgg
     1261 aacaaaccgg aactgcggga tgagttgtcc cggttcaagg tgatgaggtg tctggcatga
     1321 attgcgcccg gtttagtgaa tgcgtgcgcg attggattcg cggttctgcc ggagaagcca
     1381 tgcacgcgga gatggcggag catgccgcgg cgtgtccgga atgcgctgct atcggggaag
     1441 aagagagatt actgtcgtcg gcgctgtcgg ctctggcggc ctctacctcc tcttcggaag
     1501 ctcctgaaga agtggcgcag gcgcttttga cggagttccg cgccggccgc cgcacccaac
     1561 gcaacatacg gcggttctcc tggacggcgg cagtcgcggc cggtattggg ctggtagccg
     1621 ttatgggaaa ttggagtgtt ctgaaaccgc ggcttcaggc tccgccggct gtcgcggaaa
     1681 agcgagctgg tccagttcag actgttccgg ttcaggaagc agcgccggcg ccgcagcctc
     1741 ctgccgtcga acgtggcgcc ggcgcgcgcg ctgccagtgc cctcactgct cgcgtccgtt
     1801 caggccgcgg cgtccggcgc ccggttgcgc cccccgcgcg gcccgagccg ctgcgggagt
     1861 acgtgacgga gttcgttccg ctgcgctatg gcgaatcgtt gcggttggac gatgtggcgg
     1921 gtgttgttcg catcgcgata ccgcgctccg aactggcgcg gttcggcttg ccggtggctc
     1981 ccgaagcatc aggccggcgc attcaggcgg atgtgctgct gggtgtggat ggtgtagcgc
     2041 gggccatgcg ctttgtcaag acagagacct actagatccg tgttcgaaag gaaaaacgtt
     2101 atggaatttt cgcgtgtttc gatgttcatt gccggcgcgc tcgccttctg tgcggcgtcc
     2161 gtgctgtccg ggcaggaaac tatcgtgggc cagcggattg gctatcctcc ggcacaggct
     2221 gccgctgttc ctgcagaggg cactgcgggg ggtgtgtttc agatggcttt gcctccacca
     2281 ccctttggag gagagcccgg gacgtttagt ctcatcgcgg gacaggctgg ttttggcatg
     2341 gtcgcggtcg cgggtgcgcc ctattcggcg caggccgtaa cggagacgtc ccgggtgttg
     2401 ggggatggca cacggattca gcagaaaaac acggtggcca tgtatcgcga cagcgaggga
     2461 cgtacgcgcc gcgagcagac gttggacggc attgggccgt ggcgttcggc caccggaacg
     2521 gttacagtcg tgttcataca cgatcccgtg gcaaaagaga tgtacacgtt gaacccggcc
     2581 gacaagaccg cgacgaagtc cgttattgaa gagaggttcg atcccgcggc gggagcgaac
     2641 cggcgccgtc cgggaccgcc tccggacgag atgaaagcca ctgaagtgca ggtcatgcgg
     2701 cgtgttggcg gcggccccgg tggcggtgca gttcttgcca atcggctctt tcacgcaggc
     2761 agagctgaaa acagccagac cgagtcgctg ggacagcaga acatcgaggg cgtgatggct
     2821 gagggtacgc gcgtgaccac cacgattgag gttggcaaga tcgggaacga ccggccgatt
     2881 acgattgttt ccgagcactg gtactcgccc gatctgaaaa tgattgtaat gagcaagcgg
     2941 accgatccgc ttgccggcga tacgtcttac aaggtgacga atctcgtccg cgcggaacag
     3001 ccgcgttcgt tgtttgaaat tccgcccgac tacacggtga aagaaaccgg aggatttttt
     3061 cggcacacca tagaagtgaa gaagtaaaat ccgggcgggg caatggcgtg acacccgctg
     3121 ccccgtcaac agaactcagg cctgcctgat ccgtatcccc gcctcgccga aaagaacctc
     3181 taccatcccg aagtgctcgt tgtaatactc gctcgagtat tgacgcatgc ggacatccga
     3241 aaccaccacc tcgacgatac ccgcctgcac gatggcccgg gcgcagtcca tgcaaggcat
     3301 gatctccaca tagattgtgc atccttcggt cgcggtgccc gcgcgcgcag cattgcagat
     3361 ggcattgcgc tccgcgtgct caatccacag gtactttgcc ggacggacca cgcgctccgg
     3421 gatatcgtct cgtataccac gcggaaaaga gttatatccg gtcgaccgga tctcgtgggc
     3481 ggggcctaca attacgcagc cgatctgcgt gttagggtct ttgctccgag tggccactat
     3541 cttgcaaatg ctcagataat actcgtccca ggagggaatg acacgtgcgg cgtgttccgc
     3601 tggttgcatg ctttcgctat tgtacgcggt gcggcctcaa tgcggcactg acgtaccagt
     3661 acgggggcgg ttttttcgta ccgggacggc acgaatctac aaattcgaaa cccgtgcagg
     3721 cggttcgtca tccatatatt agggactttc ggaagaggaa ttctgagagg aacaggtctt
     3781 ttctcctggc ttttatagcg tgaagttatt gataacgttc tcatcaaagg cggccggtat
     3841 actcggccgg cgaaaaaagt tgtactggtt gtagcgcttc aggaggagaa ggcatatgaa
     3901 acggatttgg acatttctca gggatagttg gtgctaccgg atgcatccgg caccgatgtg
     3961 gccggtaaat ggaatgtatc gctgtcccga atgccagcga gagtaccccg taccgtggga
     4021 aaaaggtgtc acacgttggg attttcagcc ggctaaagag gatgtccgcg agtccaccgt
     4081 tgtgagccat cctgcacgtt ccggattccg gatcgtgaag gctttcaagc gcacggcggc
     4141 ataagtacct tcccgcttat tttccacgaa atcaaaattt tgaggcgagg ccgggttcaa
     4201 ggattgctga ccggtctctc gcccgcgatg taatcttccg tgtgagaaag aactgcacca
     4261 tttgagaaaa aaacttccag ccggaaggtg atgtggtctg acacatggct gtagagaact
     4321 gacccatttt gaaggctggc acgggtaaga ttcacggttc gcgtgctgtc gccgtcgcgt
     4381 attaccagag ctccgccaag agaacgccga atggcggggg cggagcgatc ccacgttatc
     4441 ttcacatttt cccctgaacg ttccagggcg actcccagcg tataagagtc cactgcggaa
     4501 gcctctccgg tttcgggact tgtgaaccgg gatgctacaa agccgagagc gattcccaac
     4561 agcagcaata ggaaagcgga aaacttccag acgctattcg tgagcggtac cggcatggac
     4621 ttgtttgccg ggggggcctc ggtaggctgt aatgcgggcg taaccataac ggggataggt
     4681 tgttcgggcg gagcaggcgg gtgggccggc acggtctgat gatgttgagg ctgtgccaat
     4741 gccggaatgg cgcctcgctc cttcctgacg gttggtgagc cgcccagttc gcggcggcga
     4801 aaaggaaatt ccagatagct cgattccgtt cgaaaagccg cgccttcacg gaagaaaaaa
     4861 cctgcctggc tggcttttgt ggcgaacggc ttcacaagca aggcgacggc attcggttga
     4921 ggaaaataat cgttgaaaat ttggatgtct tccggactca gtcccatccc gtcccgcgtg
     4981 tggctgcgaa agtagccgac gacatggctc ttccggccag gcgatggttt ccaggcctct
     5041 atcgtggtac gcagccgctg taactcgggt tcggagaggg tgaaggatgg accgctacgg
     5101 tactcgcaag gaacgcgctc aaacccatcg atgacaatga ccgtgccatg gtcctctttg
     5161 acactgccca gcaggatgcc cccgacctcg gcgccacggc gcggaaccgc gccgaaaccg
     5221 ctcattacct cttgcaacag cgcatcgact aaatccagat tcagccggac cgcgatgctc
     5281 ttccccgcga cttgccagac gtagtaaaca gggaagctgt ctcgaggttc ggacgtaggt
     5341 gccgtgttgt ccattcattc tcgccgaaac cacatgttgc cgcgcggcac gacccgctcc
     5401 atcattttat cgacagaagg ctggaaaact acaatgtgtt aatggccgtc tccggcactc
     5461 tcttcgttgt gtcgacaccg atcggaaacc tggatgatct gacatttcgc gcactacggg
     5521 ttctcaagga ggctggagtc atcgcttgcg aggacacgcg gcacacgaag aaacttctgg
     5581 atcactatgg catcaagcgt ccactggtaa gctaccacga gcacaacgag aacgaacgtg
     5641 ccgtggaact ggtaggccgg ctacagcgcg gcgaaagcat cgcgctggtc tccgacgccg
     5701 gaacaccgct gctgtccgac cctggttacc ggatcgtggc cagggcaagg caggagggaa
     5761 ttccggtggt tcccttgccg ggcgcttccg ccttgcttgc ggcactggca gcctcgggtc
     5821 tgcctacgga cgcctttcac ttcgccgggt tccttccgcc aaaacctgct gcgcggcgaa
     5881 agcttctcga gtcccttcgt gacgagcacg cgacgcttgt gttttatgag gctccgcatc
     5941 gcattctgga agcgctcgcg gatgtcgaag cgattctcgg acagagagat gtagtagtgg
     6001 cgcgggaact caccaaagta catgaggagt tcctgagagg aaccgccgcc gaaatcgggc
     6061 agttgctcgc cgcgcgcggc agtgtaaagg gcgaaatcac gctgctggtg gcgcgcggcc
     6121 ggcaagccag cgccggtagc ggatcggtcg aagaagccgt tgacgcgttg tccgccgccg
     6181 gcatgccgcg catggaggct atcaagacgg ttgcccggga gcgcggcctg tccaagcgcg
     6241 aagtctatcg cagggcccac gagccggaac actgaaaggc gcccttcgat accatggcac
     6301 tcatgaatat gcttcgccgg atccgctgca cggctctgtt tctatgtgtc tcgaccgtac
     6361 tattctccca aacaaaacca cgcctgattg tttttacgga tatcggaggg gatccggacg
     6421 accagcagtc gatggtacgg ctcctgtcct atgcaaacga gttcgaaatc gagggattga
     6481 tcgcgtccgc cgccggcatt cccggagagt tgaaggagaa cgtggtccgg cccgacctca
     6541 tccgtcagat ggttgccgct tacggccacg ttcgccccaa cctggagcgc aacaaaccgg
     6601 gatttccgtc tgccggctac ctcgagacac gtatcaagtc cggcaatccc aagcgcggtg
     6661 tcgaaaacat cggtgcaggg aaggacaccg aaggttccaa ctggattatc aaggtcgtgg
     6721 atagggacga cccgcgtccg gtgaatgtcg ctatctgggg tggggcgacg gaactggcgc
     6781 aagccttgtg gcgtgttcgt aatgaccggg gagcggcggg ggtgagccgg ttcgcggcga
     6841 agcttcgcgt acactcgatc tcgcaccagg acaataccgg cccatggatt gtggagaact
     6901 ttcccgagtt gttcttcatc ctgaatgcga aagccgcgga ggacgtattg cgtgttacgc
     6961 cttccggccc cgaccggcgg ttgtcctctt atcgcggcat gtacctgggt ggcgatgagt
     7021 ctctcacatc gcgggagtgg atcgacacca acgtgcgcca caaccacggt ccgctgggcg
     7081 cgttatatcc gcctaagaca tggaccgtgc cgaatccaca cagcgccctg aaggaagggg
     7141 atacaccgtc ctggttttac ttcctggcga acggcctgag cgaccctgca cacccggaat
     7201 ggggcggatg gggcgggcgc tttcttccag ccggaggcgg cctctatcgc gatgccgtcg
     7261 atacggtgga cggggtaata gatgcccggg cgaccgtgtg gcgctggcgg ccggcatttc
     7321 aaaacgattt cgccgcgcgc atggattggt ccgttcagcc accggacaag gccaatcaca
     7381 atccgatagc ggtattgaac ggcaaccgca cgcagggaat tgtgaaggtc gaggtttcat
     7441 ccggcgcacc ggtcactctg tccgccgccg gttccgccga ccctgacgga gaccgcctgt
     7501 cgttccgctg gttcctttac ccggaggcgg gcacttataa aggtgccgtt cccatcgaaa
     7561 acgccgaaga agccgtggcg cggattgtcg cgcccggcgt acagtcgccc gcgacggtgc
     7621 acgtcattct caaagtgcgc gacggtggaa ctccggcgct ctacgcctat cgcagggcca
     7681 tcttgactgt acagccgaaa ccgaaggcgg ctatcgcggg agagcgcaag ctgtggcata
     7741 aaatcacgat cacgttcgac ggcccgtctc tctccgagga agcgatgccg aatccgtttc
     7801 gtgattaccg tctcaacgta atgttcacgc ataccagcac aggggtgcgt cgcgccgtac
     7861 ctggttattt cgccgcggac ggcaacgcgg ctgaatcctc ggcgagcgca ggcaataagt
     7921 ggcgcgtgaa cttctcgccg gacctcccgg gtgagtggcg ctatcaggct tctttccgaa
     7981 gaggcacaga cgttgctaca agcctggatg taactgaggg tgaggccgca ggcttcgatg
     8041 gggcttcggg tacgttccgc gtggcagcgg ccggcaagcc cggcaacgat tttcgttcga
     8101 agggcgtgtt gcgctatacg ggggagcact acctgagaca tgcggagaca ggcgagcggt
     8161 ttttgaaagg aggggcggac agcccggaaa atttcctggc ttatgccgat ttcgacggca
     8221 cgttcgatac cgatgcccgg gtgaatgaag gcatgtcggc ccgcttttcc gaaccgttta
     8281 tccatcacta cgagccgcac gccgccgact ggacgcccgg cgatccgtcc tggaagaacg
     8341 gcaagggtaa aaacatcgtc ggcgcgttga actaccttgc ctcgaagggt atgaacagcg
     8401 tctatttcct cacttacaac gttgacggcg gcgatggaaa agacgtatgg atgtggactt
     8461 cgcccggcgt ccgcgatcgt tacgacgtaa gcaaactcga gcagtgggag atcgtgttct
     8521 cacacatgac gagcaaagga ttgctgctgc acgttgtgac tcaggaaacc gagaacgatc
     8581 gcaaactcgg cggcggaccc ggcttgaatc cggtccggag gctgtattac cgggaactgg
     8641 ttgcgcattt ttctcatcat cccgccatcg tctggaacct gggggaagag aacaacacca
     8701 gcgatgcgga ccggaaggag attgccgctt atcttcgcgc gctcgatccg tatgctcatc
     8761 ccatcacggt gcacacgcac aacaacaagg cgccggattt ttacaatggc gtgctgggcg
     8821 attccaactt tgaggcggcg tcgatccagg gcgacatgcg gaactattat cgcgacgcgg
     8881 ttgtgttgcg cgaacgctcc gcgaaagtag gccggaaatg ggcgatcttc ggagacgagc
     8941 agccaagcgc cgacgtgggt gtgatgcccg atgagtccga tccgggccac gatatcccac
     9001 gaattcacgc gctatggggc aaccttttag gcggtggctc cggtgtggaa tggtacttcg
     9061 gttacaaata cccgcatatg gatttgaatt gcgaagactg gcggtcgcgc gatcgcatgt
     9121 gggaccagac ccgccatgcc ctcacgttct ttcacaggca tctgccgttc tgggagatgg
     9181 agccggacaa ctcccttgca tcgagcacgt cgggtgcgag agtgtttgcc aaaaggggag
     9241 aagtttacgc ggtgcagttg ccgggtggaa ccggagactt gagcgtgccg gaaggccgct
     9301 acacggtgag ttggtataac ccacgcaccg gcggcgattt agtggcgggt tctgtggcct
     9361 acatcaccgg gcccggttcg cagtccctcg gcagcccgcc atccgagccg gaaagagatt
     9421 ggattgtact ggtgaagaag cgctgaagcg cacggctatg ccagcaattt cttgacgacc
     9481 aaaccctcga cgtccgtaag cctgaattca cggccctgga aacggtacgt gagcttcgtg
     9541 tgttcaatgc cgagcagatg caggatggtc gcgtgcaggt cgtgaactga gattgcatct
     9601 tcggcgatgt tgtagccgac gtcgtcggtg gcacccaccg taatgccggg tttgatgccg
     9661 cctccagcca tccacatggt gaacgcgcgc gggtggtggt cacgccccag aaacttggag
     9721 ccgctgcgcg cttcattcat cggcgtgcgg ccgaattcac caccccagac gaccagcgtc
     9781 gaatcgagca atccgcgctg tttcaagtcc ttcaccagcg ccgacgcgcc ctggtcggtt
     9841 tcgcggcaca ggtgcgcgag cttgttgacg atgtcttcgt tattactcgc accgtgagta
     9901 tcccaaccgc ggtggtaaag ctgtacaaac cggacgccgc gctccaccag gcgccgcgcc
     9961 agcaggcaat tgttcgcgaa agaagcgttg cccggctcgg tgccgtacat ctcgtgaata
    10021 tgttggggct ctttggagat ttccgccagt tccggaacgc tcgattgcat ccggtaggcc
    10081 atctcatagg agttgatgcg cgtggcgatt tcgggatcgc ctatatcggc caggtgatcg
    10141 gtattgaggt cacgcagcaa atcgagagaa cgccggcgcg tctccatctc tacccccggc
    10201 ggattcgaga gaaacagcac cgggtctccc tttgaacgga actccacacc ctggtaaacc
    10261 gtgggcagaa atccgctacc ccaacacgac ttcccaccgt cc
//
LOCUS       DASTMK010000363         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_81181, whole genome shotgun sequence.
ACCESSION   DASTMK010000363 DASTMK010000000
VERSION     DASTMK010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81181"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..>1936
                     /locus_tag="VFQ79_15935"
     CDS             189..>1936
                     /locus_tag="VFQ79_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141213.1"
                     /translation="MKHVPAAFGVFLLAAAVSASVKAQQNTPPPPDDASKTGEVEATN
                     LDQVIVTGVRTPKAVDKIPGAITLVSKQEVERTLGLTEDATAVLARQVPGYSEASQAM
                     SNTGETLRGRIALRLFDGIPQTSPLREGNRSGTFTDMGLVGRIEVINGPSASEGIGAQ
                     GGIINYLSKTPTKDGSETTVTSRYSTQNGDDSESWKLGVTYAYKADDFDLLTAASFID
                     RGISYDGRGRRIGMNASGSIADSKADNLFIKWGMNFGEAGEQRLQGSFSRFNVQGNNN
                     YHWVPGDRERGITDTAERGRIPGSKAEFNDFTQFAFSYTHGDFFAGTLKVDLYKADQA
                     MRYPAENGDDRQDPEIAPLGTLWDQSEIHSNKQGLRTAWTRPQLFGVDGLEMRVGLDL
                     VEDETEQSLALTHRVWVPPMTYKSTAPYMQLSYDIGPVTLSGGLRREDGELSVDDYTT
                     TWFRDRRFVQGGKLSYEETLANFGAIWRLPAGFSVFASYGEGFGLPNVGIPLRNLQCS
                     NDSPEGTQPDGCPNDPQATVAGIVDLQPIIADNREIGLNWQGQRGSFGLSHYKSDSDF
                     GVSLAVDPISRDFVMVR"
BASE COUNT          383 a          597 c          594 g          362 t
ORIGIN      
        1 tttccgtgac ggcgtgcacc taggctctgt cgacattgtc gaggctgtcg ccggcgtctg
       61 ggaggaacgc ctggcagcgc gggcgctttt cctgccgcct gcggatgcga aaacacaaga
      121 acgccatcgg ggagccctgc gtgccgcatg ccggggttct tgcagccacc atctcagaga
      181 agaacaccat gaagcacgtg cccgccgcat tcggcgtatt cctgctggcc gccgccgtca
      241 gcgccagcgt caaggcccaa cagaacaccc cgccgccgcc ggacgacgcg tcgaagacag
      301 gggaagtgga agccaccaac ctcgatcagg tcatcgtcac cggcgtgcgc acgccgaagg
      361 cggtggacaa gatcccgggc gcgatcaccc tggtgtcaaa gcaggaagtc gagcgcacgc
      421 tcggcctgac cgaggatgcg acagccgtat tggcgcgcca agttcccggc tactccgagg
      481 cgtcccaggc aatgagcaac acgggtgaaa cgctgcgcgg tcgcatcgcg ctgcgcctgt
      541 tcgacggcat tccccagacc tcgccgttgc gcgagggcaa ccgatccggc accttcaccg
      601 acatgggcct ggtcggccgc atcgaggtca tcaacggacc ttcggcctct gagggcatcg
      661 gcgcacaggg cggcatcatt aactatcttt ccaagacccc gacgaaggac ggtagcgaga
      721 ccaccgttac ctcgcgctac agcactcaga acggcgacga cagcgagagc tggaagctgg
      781 gcgtgactta cgcgtacaaa gctgatgact tcgatctctt gaccgcggca tcctttatcg
      841 atcgcggtat ttcctacgat ggccgcggtc gtcggattgg catgaacgcg agtggctcga
      901 tcgccgattc caaggcggac aacctgttta tcaagtgggg catgaacttc ggcgaggcag
      961 gggagcaacg tctccaaggt tcgttcagcc gcttcaatgt gcaagggaac aacaattacc
     1021 attgggttcc cggcgatcgc gaacggggca tcaccgatac tgccgaacgc ggccgcattc
     1081 cagggtcaaa ggcggagttc aatgatttca cccagttcgc tttctcctat acgcacggcg
     1141 atttctttgc cggaaccctg aaggttgacc tgtacaaggc ggaccaggcc atgcgttatc
     1201 cggccgaaaa tggcgatgat cgccaggatc ccgagatcgc gccgcttggc acgttgtggg
     1261 atcaatccga aatccattcc aacaagcaag ggctacgcac agcctggacg aggccgcagt
     1321 tgtttggcgt tgacggcctg gaaatgcgcg tcggcctgga cttggtagag gatgaaaccg
     1381 agcagagcct ggccctgaca catcgtgttt gggtgccccc gatgacgtac aagagcaccg
     1441 cgccttacat gcagctctcc tatgacatcg gcccggtaac gttgagtggc ggtttgcgcc
     1501 gtgaagacgg cgagttgtcc gtggacgact acaccaccac gtggttccgt gaccgccgct
     1561 tcgtccaggg tggcaagctg tcctacgagg agacgctggc caatttcggc gccatctggc
     1621 gactgccggc cggattctct gtcttcgcgt cctatggcga ggggtttggc ttgcccaatg
     1681 tcggcatccc gctacgcaat ctgcagtgct ccaatgattc gccggaaggt actcagccgg
     1741 atggctgccc caacgatccg caggccacgg tggcgggtat cgtcgatctg caacccatca
     1801 tcgccgacaa tcgtgaaatc ggtttgaatt ggcaggggca acgcggcagc ttcggtctgt
     1861 cccactacaa gtcggattcg gactttggcg tttccctggc ggtggacccg atcagccgcg
     1921 atttcgtgat ggtccg
//
LOCUS       DASTMK010000364         4528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_498427, whole genome shotgun sequence.
ACCESSION   DASTMK010000364 DASTMK010000000
VERSION     DASTMK010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498427"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2224
                     /locus_tag="VFQ79_15940"
     CDS             <1..2224
                     /locus_tag="VFQ79_15940"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU0141214.1"
                     /translation="SFLLEVEKEGFQTATTNIGVPPATPSGGDVTLRVAGVAESVVVT
                     AAGLPQQLDEISKAVSTTTREEIQDRNEYTFSEILRTTPGVLITNGGGPGQNTSIRIR
                     GVRPDATAILVDGLPFRDASTTQSDASSFVSNLNFITTGRVEVLRGSGSSLYGTNAVG
                     GVINVVTDEGGGRVHGDLQTEGGNLGFWRGRGRIGGGLLGDRLTYSAGFLHLNVTSGV
                     DGDDATRSTGGQGFLKYNLAPTASLSGRLWASDDFVQLNTGPTTSGIPAANIPATGII
                     PAIPLSPEGVRIVNDGGTPNYGNATYIPGRNDPDDRRASRFYSTALIFRHAPAPRVSW
                     QASYQRVHTNRVYNYGPAGTGFQNSTRNDYLYVGDIDTLDVRGTALPASWLSLTGGYE
                     FEHEGYHERANNNAPGASLIDTRSNISQNGHAAYFASQVSLLSRRLQLSFSGRAQVFR
                     LTEPEFDAKGVATPYEGLAVKSPPRALTGDVSASYLIAASNTKIRAHFGNAYRAASIY
                     ERFGGGFFNDFFTNELVFNAYGDPRLSPDRYNSVDGGIDQYLFQDRLRISATAFYSRV
                     VSITAFANAIDGTTDPFGRFYGYINGSGGISRGVEFGIEARPVRSLILNASYTYTNAD
                     LDRDITVAGFWKVLGVPANMATLVATKQWTRRLSTNIDLFTSSKYYTSFSAGGRSRAF
                     EFPGFTKVDLTANYRIWEGETRAVRLYGKVDNMFNERYYQNGWLNPQATFVMGLGYTF
                     "
     gene            complement(2230..3690)
                     /locus_tag="VFQ79_15945"
     CDS             complement(2230..3690)
                     /locus_tag="VFQ79_15945"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEU0141215.1"
                     /translation="MFPRSASTDWQSAKMEEVARSIFIGGTGSNVGKSWMATALCRLL
                     VRRGVRVAPFKAQNMSNNSHPCPEGGEIGRSQVAQAEACGLAPMVKMNPVLLKPAGDS
                     RSQVILHGKVWQTIDAREYYHHAPFLRQEALKAYEALASSFDIIVIEGAGSVAEINLW
                     DRDFTNLSMAQAVGARSLLVADIERGGVFASILGTLDLLPKEYRMLFRAFAVNRFRGD
                     RSLFETGVTILEEKSGLSCLGVFPFAPDLHLDAEDSLASPSNVQKTEQPAQIAVIRFP
                     RISNSTDFRLLPNADWLDRPNGLHYRFIILPGTKSTIADLEWLRRAGLAHWIIEQHKS
                     GATIIGICGGYQMLGETIDDPHGLDGTPGSVAGLGLLPVHTRMEPEKTTAKREACTPA
                     GVRFQAYEIHMGRTVSELSAQPFAILADGAREGIRRNRVIGTYLHGALESPDVVAELF
                     ECRPPVDPVKDRHYDALADWLELSASPGVLAELIEG"
     gene            complement(3762..4355)
                     /locus_tag="VFQ79_15950"
     CDS             complement(3762..4355)
                     /locus_tag="VFQ79_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0141216.1"
                     /translation="MPQLKELLVEQLQDLLHAEGQLTGALPKMAEAAHHPKLKEAFEK
                     HLTQTETHVERLRQVFEMLGEEAQGKPCKAMIGLIEEGNETIEEGADKEPVAADLALI
                     AAAQRVEHYEIACYGTVRNLARQIGEIDAARLLTHTLGEEESTDFLLTAISDPLTQQA
                     AIEDGGNGVNLENTEKRGPARATKKTEKSGKMVSRAR"
BASE COUNT          902 a         1380 c         1264 g          982 t
ORIGIN      
        1 aagcttcctg ctcgaagtcg agaaggaggg ctttcaaacg gccactacca acattggtgt
       61 cccgccggca acgccttccg gtggcgatgt caccttgcga gtggctggag ttgccgagtc
      121 agtggtggtc accgccgccg gtctgccgca gcaactcgat gagatttcga aggcagtgag
      181 caccacgacg cgagaggaga tccaggaccg caacgaatac acgtttagcg aaatcttacg
      241 caccaccccg ggcgtgttga ttaccaacgg aggcggccct gggcaaaaca ccagcattcg
      301 tatccgcggg gtacggcccg atgcgacggc gatcctggtc gacggattgc cattccggga
      361 cgcctctaca acgcagtcgg atgcaagctc gttcgtgtcg aacctcaact tcatcaccac
      421 cgggcgcgtc gaagtgctgc gtgggtccgg gtcgtctctc tatggcacga acgccgtcgg
      481 tggtgtcatc aatgtagtta ccgatgaagg cggcggccgg gtgcacggcg accttcagac
      541 cgagggtggc aatcttggtt tttggcgcgg acggggcagg attggtggtg gacttcttgg
      601 tgaccggctt acttactcag caggatttct ccacctcaac gtgaccagcg gcgtagatgg
      661 cgacgatgcg actcgcagca cgggagggca gggatttctc aaatacaacc ttgctccgac
      721 tgccagcttg agcggacgcc tttgggcttc cgacgatttc gttcagttaa acacgggccc
      781 cactacttcc ggtattcccg ccgcgaacat cccggcgacc ggcatcatcc ctgcgatccc
      841 gctatcaccg gaaggagttc ggattgtgaa cgacggtgga actccaaact acggcaatgc
      901 aacttacatt cccggccgca atgaccccga cgatcgccgg gcatcacgct tctattcaac
      961 tgctctcata ttccgtcatg ctccggctcc acgcgtcagt tggcaggcca gctatcagcg
     1021 ggtacacacc aaccgcgtgt acaactatgg tccggcaggc accggttttc agaactccac
     1081 gcgaaacgac tatctctatg ttggcgatat cgacacgctc gatgtgcgtg ggaccgcatt
     1141 gccggcatca tggctgtccc tgacaggggg ctatgagttc gagcatgagg gctaccacga
     1201 gcgggcgaac aacaacgccc cgggtgcgag tctgatagac acacgaagca acatcagtca
     1261 aaacggacat gccgcctact ttgcttcgca ggtttctttg ctcagccgcc ggttgcagct
     1321 ttcgttttcg ggtcgggccc aggtgttccg gttaacggaa ccggaattcg atgcgaaagg
     1381 cgttgctaca ccctatgaag ggcttgctgt taagtcgcct ccccgcgccc tgacaggcga
     1441 cgtttccgca tcttatctga tcgccgcatc aaatacgaag atccgggcgc atttcggcaa
     1501 cgcctatcgt gcggcgtcga tctacgagcg tttcggcggc ggcttcttca acgacttttt
     1561 tacgaacgaa cttgtgttta acgcctatgg cgatccacgt ctctctccgg accggtacaa
     1621 ctccgtcgat gggggaatcg accaatatct ctttcaggac cggcttcgca tatcggccac
     1681 tgcattctat tcgcgtgtcg tctccatcac tgcttttgcg aatgcgattg acggcacgac
     1741 agatcccttt ggccggtttt acggctatat caacgggtcg ggcggcatct cacgcggagt
     1801 ggagttcgga atcgaggcac ggcccgttcg atccctgatc ctcaacgcct cctataccta
     1861 taccaacgcc gatctcgatc gggacatcac cgtcgcaggt ttctggaaag tgctcggcgt
     1921 accggcgaac atggcaacgc ttgttgcgac gaaacagtgg acgcggcgcc tttccacaaa
     1981 catcgatctg ttcaccagct cgaaatatta cacatcgttt tccgcgggcg gccgctctcg
     2041 agccttcgag tttcccggtt tcacaaaggt ggatcttacg gccaactacc ggatctggga
     2101 gggcgagacc cgcgcagttc gcctttacgg caaagtcgac aacatgttca acgaacgcta
     2161 ttaccagaac ggctggctta acccgcaagc tacattcgtg atgggtctcg ggtatacctt
     2221 ctaaaccagc tagccctcga tcaattcggc gagtacaccg ggagaggcac tcaattcgag
     2281 ccaatcagcc agcgcgtcat agtgacgatc cttgacggga tcgaccggcg gccggcattc
     2341 gaacagctcg gcaactacgt ctggtgattc gagcgcgcca tgtaggtaag taccgatgac
     2401 acggttccgg cgaatccctt cgcgggcgcc atcggcaaga attgcgaacg gctgggcaga
     2461 tagctcggat acggtccttc ccatatggat ctcgtacgcc tggaaccgaa ccccggccgg
     2521 agtacatgct tcacgttttg ctgttgtctt ctccggctcc atcctggtgt ggaccggcag
     2581 caggccgagg ccggctacag atcctggcgt gccgtcgagt ccatgcggat cgtcaatggt
     2641 ctcgccaagc atctgatagc ctccacagat cccaatgatc gtcgccccgg acttgtgctg
     2701 ctctataatc cagtgggcca gcccggctcg tctgagccac tccaggtccg caatagtcga
     2761 cttcgtgcca ggcaagatga tgaaacggta atgcagcccg tttggccgat ccagccagtc
     2821 ggcatttggc aggagccgga aatcggtcga attcgagatc ctcggaaatc ggatcaccgc
     2881 aatctgcgcg ggctgctctg tcttttggac gttcgatggg gatgcgagcg agtcctccgc
     2941 gtccaggtga agatccggtg caaatggaaa gacaccgaga caagacaggc cggatttctc
     3001 ttcaagaatc gtcactcctg tctcaaatag agagcgatcg ccccggaatc gattcacggc
     3061 gaaagcgcgg aacagcattc gatattcctt cgggagtaaa tcgagagtgc cgaggatcga
     3121 tgcaaagacg ccgccacgtt cgatgtccgc gacaagaaga ctccgagccc ccaccgcctg
     3181 ggccatgctg agattggtga agtcccggtc ccaaagattg atctccgcaa cgcttccggc
     3241 gccttcgata acaataatgt caaacgacga tgcaagcgcc tcgtaagcct tcagcgcctc
     3301 ttgccggagg aagggagcat gatgataata ctcgcgagca tcaatcgttt gccacacctt
     3361 gccgtgcagg atcacttgcg aacgagagtc accggccggc ttgagaagca ccgggttcat
     3421 cttcaccatc ggtgccagac cgcaagcctc cgcttgtgcg acctgggacc ggccaatctc
     3481 accaccctca ggacagggat gagagttgtt ggacatgttt tgggctttga acggagcgac
     3541 ccgcacgccc cggcggacca gcagccggca cagagccgtt gccatccaac tctttccaac
     3601 gttcgacccg gtgccaccaa taaagatcga tcgagccact tcctccattt tcgccgactg
     3661 ccagtctgtg ctcgcagaac gagggaacat tggtgaaaac ctggaaaacc gcttgctggc
     3721 gcgcgcggct ccgtttctga gcagcgacct gcctactcct gttagcgggc tcgactgacc
     3781 atttttcctg atttttcagt ctttttcgtt gcgcgggccg gccccctctt ctccgtattt
     3841 tcgagattga caccgttgcc gccatcctcg atggcagcct gctgggtcag cggatctgaa
     3901 atggcggtca gcagaaaatc ggtgctttct tcctcgccca gtgtatgcgt cagcagccgc
     3961 gcggcatcga tctcgccgat ctgtcgtgcc agattgcgga ccgtgccata acaggcgatt
     4021 tcgtaatgct cgacccgctg cgcggcggca atgagagcga gatcagcggc taccggctcc
     4081 ttgtccgctc cctcttcaat cgtctcgttg ccctcttcga tcaggccgat catggccttg
     4141 cacggttttc cctgcgcctc ctcaccgagc atctcaaaca cctggcgcag gcgctccaca
     4201 tgggtttcgg tttgcgtaag gtgcttctca aacgcctctt tcagcttggg atggtgggcc
     4261 gcctcggcca ttttgggtag cgcccccgtc aattggcctt cggcgtgaag caggtcctgc
     4321 aattgttcga cgagtagttc cttgagttgt ggcatggaat gtccctttca gatttgttag
     4381 ctcttcacag cgccgttgcc acccggctgg cgggtgccgg cctgcgccga ttcgaattcc
     4441 tggaaagcgg gagccgtcac catctcccag ccgttgccct cgttccaggg gcctttcgca
     4501 tccggttcgc cctggtcacc ggtcccgg
//
LOCUS       DASTMK010000365         8557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_337454, whole genome shotgun sequence.
ACCESSION   DASTMK010000365 DASTMK010000000
VERSION     DASTMK010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337454"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFQ79_15955"
     CDS             complement(<1..199)
                     /locus_tag="VFQ79_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141217.1"
                     /translation="MKRLALLAILVFQAEAAEWVRIATTAFEVYTDAGEKTAREALAQ
                     IERIHSAFAPMRSRSLPLPARM"
     gene            284..910
                     /locus_tag="VFQ79_15960"
     CDS             284..910
                     /locus_tag="VFQ79_15960"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEU0141218.1"
                     /translation="MEQSAWDAGLLYRIGKIANSRRTLDEALNEILALLVQRTGCQAC
                     FVYVPDRSSGERILRASHAPPSWEPGSIKERALSVPLTGSNHSQGVLDIYFHGHRKAT
                     PAEVTFAAFVAQQLGGAMERAYLEAENARLLEEVRRTRSQLEVRKVVERAKGILQRRL
                     HLTEEEAYLRLRNESRRLRTPMKQLADAIILQESQDAGAKSFLNWFNS"
     gene            complement(911..4096)
                     /locus_tag="VFQ79_15965"
     CDS             complement(911..4096)
                     /locus_tag="VFQ79_15965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0141219.1"
                     /translation="MEPAGDDHKNLALRLSEFSIRHPVTTWMIFASFIVLGTVSATKI
                     PLVMAPDIKFPFVGVHVPYPNATPEQIQETITKPVEEAVSTIPHIQLLRSRSSADEAW
                     IDLNFEWGADMEWLRAEVREKVEQIRHELPADVERIRVMNFGTEDIPILEGRIATKRD
                     LRHAYDFLEAKVKRPIERVPGVGEVEMWGAVRNEIDIYLRLDDVKRHRVEVGALFRRL
                     DSVNLDRSLGRVVDRDARYSAMTRGTLKSLDDIRNFPVNSQGLRLSQVADIIYDNPPI
                     NYGRHLNGEYAIGFGVRKTSEANTVETVRRVLARIEEINRDPSLGGVQILVWHDSGKE
                     ITKSLSGLLEAGTIGALLAVVVLFLFLRKVGATLAIGFAIPFSMIATVGFLYLLGKTL
                     NVLSMMGLMLAAGMLVDNAVVVLESIYQNLEKGRDRVSAAVTGAREVITAVFAATLTS
                     IIIFVPLVFGKATNYSMFLADTGTSIMIALLCSLFISLTLIPLGMARTLNVDISKRSR
                     FDEWLYRHVAPLGRRLVRRRASPAPDDAKGGSVVELYLRMMAFPLKHKFLVGFVLIPA
                     VFAVSLIVLKKVPDNSPEAQDLQDLAIQYNFTENYHYAKIERDFVAPVERYLLRNREK
                     FKIKDVSSNFGNNNAFSQVYFDKDRVTLEELKDIRQEIAKGLPVIPGAEIRLGRQEGA
                     ERLSWIGVNLYGDDSTVLQSLVREAKSRLLRHKGFIEVHNDLDRGKEEVQLRMRRDLA
                     RKYNVSPQSVANVLGIILRGQQIRGYSTPEGEVDIYMRLRPGDREDLDDLKSMVVGSG
                     SNGRDVLLEQVADLQIVKTPGVIQREDRRNYSWMWANFSGGQKDEGKEQMTQVMNSID
                     YPQGYGWSFGFWTKRQDQEDNDFLFNILLALFMVYFVMAALFESLAHPFAIMLSLPFA
                     MVGVVWTLYLTGTPFNLMAKIGLMVLVGIVVNNGIVLLDHVNNLRRAGMNRSDAILRG
                     CRERFRPILMTATTTIVGLFPLAVGTSGMFELRYFPLARTVMGGLAASTVLSLIVLPT
                     YYELFDDLAQYLKRMWYSTEPEASETRAYGD"
     gene            complement(4114..7578)
                     /locus_tag="VFQ79_15970"
     CDS             complement(4114..7578)
                     /locus_tag="VFQ79_15970"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0141220.1"
                     /translation="MNVIRSAIHRPVTVSMFVVAVILFGWVSLDRLALNLLPDISYPS
                     LTIQTDYEDAAPEEVEALITRPIEESVGVLPGLTRLSSVSRPGQSEVVLEFGWDTNMD
                     LAALDTREKLDFVTLPRDAGRPVILRFDPSYDPIMRLQLSGTGMSLSRLRYAAERELK
                     RQLESTSGVAAIKVIGGLEEQIRIEIDEKRLAELGIPITEVTRILAQENLNQASGSLY
                     DLDANYMVRMLNQFRDVEEIRGIVVRDEAGRKVVLGDVAKVWRGAKDREIIARINGKE
                     SVEMAIYKEGDANTVTVARAVQDRLDALKRANLIPRGVEYQVVFNQADFISDAIEDIH
                     SSAILGGLLAILVLYLFLRDMRSTLLIGITTPISIMATFALMYQTGITLNLMSLGGVA
                     LGVGMLVDNSIVVLESVFRHKRQGTKLDDAVFRGTKEVAMAVTASTMTTVAVFLPLIF
                     IEGIAGQLFKDQALTITYSQIVSLIIGLTLIPMLLALQVNKHAPEPQDNVAPRREPSS
                     RRWVRALQRPAWFLETKARAVGAFVFTDMVRIVVADLRRLVRMLGRLVLWPLNPVLDR
                     FDSGYKWASGRYPGLLDWALNHKITVIGFALGLAAVAAVLVPFIGAELIPPLTQGEFV
                     FELRLPEGRALEHTDRIVNGMEKRIAVLPGVHTVFSSVGGSNRNQFARETREENVGQL
                     YVVMSDKNDKRLEEQTINRIRAELRQLPEVSFTFSRPTFFSFKTPIEVEIYSQDLGNL
                     RTTADLVARRLETMRGLSDVRTSTELGNPEIQVRFDRDRLARLGLDEGEIAATVRNKV
                     RGDVASRFREGDKQIEILVRADESDRNRVENIRNLTINMGGNARVLEETPLTETRRNT
                     NGGSIAVPAPVTRQQAGGAAGGAVVQARTIPIRLGAVADVSVQRGPSEVRRIRSQRAA
                     LVSANLSGRALNSATEEIEAELQRLRASLPADTTVTIGGQNEELRTSYRSLVLAMALA
                     VFLVYLVMASQFESLVHPFIILFSVPLGVIGVIFALALTGTTFNVMVFLGLIILAGIV
                     VNNAIVLIDYANQLREQGLPRREALKQAGEVRLRPILMTTLTSVLGLVPMALGWGEGA
                     EIRTPMAITIMGGLSFSTLLTLIFIPVLYEMIDRKRYAVDEVTAHAVSDRQLTAGQHS
                     PGLPETAQ"
     gene            complement(7623..>8557)
                     /locus_tag="VFQ79_15975"
     CDS             complement(7623..>8557)
                     /locus_tag="VFQ79_15975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0141221.1"
                     /translation="AEEGDLIKEGQVLCELDDAQLKIRLELARERLAQAKLQMEKART
                     RQEKAAVQILNARTELERYQKAYKEGLASELDVAQRKYRLDELEHDERVSTQEIREFT
                     HRVAELEAEIAQAELELSRTRIRAPFSGYITHRTVELGQRIRNLDALFTIGSFSPLFV
                     DVHLSELDAQEVHPGQPALVRLGADAGAELKGQVERLSPIVDQSTGTVKVTIVMKPVA
                     ANFRPGAFVRVDIRTDTKPGAILVPKRAVIEEDGQNYVFVANGTTAKRTKVDVGYSSE
                     GVVEIRSGIRAGEQVVVAGHGALKEGSKIRSIQS"
BASE COUNT         1710 a         2719 c         2416 g         1712 t
ORIGIN      
        1 acatgcgagc gggcaacggg aggctgcggc tgcgcatcgg tgcaaacgcg ctatggattc
       61 tctcgatctg ggcgagcgct tcgcgcgcgg ttttttcgcc cgcgtccgtg tacacctcga
      121 acgcagtggt ggcgatccgc acccattcag ccgcctccgc ctggaaaacg aggatggcaa
      181 gcagcgccag ccgtttcatt ccactgtcaa gtatagggcc aatagaaact ttcgattgga
      241 cgctttccgc cgtgcaaact agtctcgaaa gtggtggaag tgtatggagc agtccgcgtg
      301 ggatgccggc ctcttgtatc ggatcggaaa gatcgccaac tcgcggcgga ccctggacga
      361 agcactcaac gaaatcctcg ctctcctcgt tcaaaggaca ggctgccagg cgtgttttgt
      421 ctacgtcccg gaccgctcct cgggtgaaag gatccttcgc gcctcgcatg cgccgccatc
      481 gtgggaaccc ggttccataa aagagagagc attatcggtg ccgctgaccg gcagcaacca
      541 ctcacagggt gtcctcgata tctattttca cggccatcgg aaagcgacgc ccgccgaagt
      601 cacgtttgcg gcgtttgtgg cgcagcagct tggcggcgcc atggagagag cttatctaga
      661 ggctgaaaac gcgcgattgc tcgaagaggt tcgccggacc cgctctcaac tcgaggtccg
      721 caaagtggtg gaacgtgcca agggcattct gcaacggcgc ctgcatttga cggaggagga
      781 ggcttatctc cggcttcgca acgaaagccg ccgcttgcgg acgcccatga agcagctcgc
      841 cgatgccatc attcttcagg aatcgcagga cgccggagcg aagtccttcc tgaattggtt
      901 caatagctga tcaatcgcca tacgcccggg tttccgaagc ttcgggttcc gtcgaatacc
      961 acattcgctt cagatactgg gccaggtcgt cgaacaactc gtagtaggtc ggcagaacga
     1021 tcagcgaaag cacggtggaa gcggcgagcc ctcccatgac ggtgcgcgcg agcgggaagt
     1081 atctcaattc gaacatcccg ctggttccga cagccagcgg aaacagacct acgatcgtcg
     1141 tggtcgcggt catgaggatc ggacggaaac gctcgcggca gccgcgaaga atcgcatcgg
     1201 accggttcat gccggcacgg cgcaagttgt tcacatgatc gagcagcaca atgccgttgt
     1261 tcacgacgat gccaaccagc accatcaacc cgatcttcgc catcagattg aacggcgttc
     1321 ccgtcaggta tagagtccag accactccca ccatcgcaaa cggcagcgac aacataatgg
     1381 cgaacgggtg tgccagcgat tcgaaaagcg cagccatcac gaaatacacc atgaacagcg
     1441 ccaacaggat gttgaacagg aaatcgttgt cctcctggtc ctgccgcttg gtccagaagc
     1501 caaagctcca gccgtatccc tgcgggtaat cgatactgtt catcacctgc gtcatctgct
     1561 ctttcccttc gtccttctgc ccgccggaga agttggccca catccaggag tagtttctcc
     1621 ggtcctcgcg ttggatcacg ccgggtgtct tgacgatctg gagatcggcc acctgctcga
     1681 gcagcacatc acggccgttc gagccgcttc ccactaccat cgacttgagg tcgtccaggt
     1741 cttcccggtc gcccggacgc aaccgcatgt atatgtccac ctcgccttcg ggcgtgctgt
     1801 atccccgaat ctgctggccg cgaagaatga tgcccaacac attggcgacg gattgcggcg
     1861 aaacgttgta cttccgtgcc aggtcgcgcc gcatccttag ctgtacctcc tccttacccc
     1921 ggtccaggtc attgtggact tctataaatc ccttgtgcct cagaaggcgg cttttggctt
     1981 cccgcaccag cgattgcagc accgtactgt cgtcgccata taagttcacg ccgatccacg
     2041 atagccgttc tgcgccctcc tgcctcccca gcctgatctc cgcgccggga atgacaggca
     2101 gtcccttggc gatctcctgc cggatatctt tcagctcttc gagcgtcacg cggtctttgt
     2161 cgaagtagac ctgcgaaaaa gcgttgttgt tgccaaagtt gctggaaaca tccttgattt
     2221 tgaacttctc cctgttccgc agcaggtacc gttcgaccgg agccacgaaa tcgcgctcga
     2281 tcttggcgta gtggtagttc tcggtgaagt tgtattggat ggccaggtcc tgaagatcct
     2341 gcgcctccgg ggaattgtcg ggcacctttt tcaggactat cagagacacc gcaaagacag
     2401 ccgggatgag cacgaagcca accaggaatt tgtgcttcag aggaaacgcc atcattcgca
     2461 ggtacaattc cacgacgctt ccgcccttcg cgtcatcggg tgccggcgat gcgcggcggc
     2521 ggaccaggcg cctccccaac ggcgccacgt gacggtacag ccattcatcg aaccgcgacc
     2581 gctttgagat atccacgttg agcgttcttg ccatgcccag cggaatcagc gtgagcgaga
     2641 tgaacagcga acacagcagc gcgatcatga tcgacgtacc ggtgtccgcc agaaacatgg
     2701 aatagttcgt tgccttgcca aaaaccagcg gaacgaaaat gatgatggac gtcagcgtgg
     2761 ccgcgaacac cgccgtgatc acttcccgtg cgccggtgac cgcggccgag acgcgatctc
     2821 tgcccttctc caggttctgg taaatggatt cgagcaccac taccgcgttg tccaccagca
     2881 tgcccgcggc cagcatcaga cccatcatcg aaagaacatt caaggtcttt ccgagcaggt
     2941 aaagaaatcc gaccgttgcg atcatcgaaa atggaatcgc gaacccgatc gcgagcgtcg
     3001 cgcccacctt tctgagaaac aggaacagca cgacgacggc gagcaatgcg ccgatcgttc
     3061 ccgcctccaa aagaccggaa agcgacttgg tgatttcctt cccggaatcg tgccacacca
     3121 gaatctggac gccaccgagc gacgggtcgc ggttgatttc ttcaatccgg gccaacacac
     3181 ggcgcaccgt ctcaacggtg ttcgcttccg aggtcttgcg aacgccgaag ccgatggcat
     3241 actccccgtt caggtggcgg ccgtagttga tcggcggatt atcgtaaatg atatcggcca
     3301 cctgcgacag ccgaagcccc tggctgttga cgggaaaatt tcgaatgtcg tcgagagatt
     3361 tcagcgttcc gcgcgtcatc gcgctatacc gtgcatcccg gtcgacgaca cgtccgagcg
     3421 accggtcgag gttgacgctg tccagcctgc ggaatagcgc tccaacctcc acgcgatggc
     3481 gcttgacgtc gtcgagccgc agataaatgt caatctcgtt gcgaaccgcg ccccacatct
     3541 ccacttcgcc gacaccggga acgcgttcta tcggccgctt caccttggct tccaggaagt
     3601 cataggcgtg ccgcaggtcc cgctttgtcg cgatccggcc ctctagaatc ggaatgtcct
     3661 cggtgccgaa attcatcacc cggatccgct cgacatccgc cggcaactca tgacggatct
     3721 gctcgacctt ttcccgtact tccgcccgca gccactccat gtcggcgccc cactcgaaat
     3781 tcaggtcgat ccaggcctcg tcggcgctcg accgcgaccg cagcaactga atatgcggga
     3841 tggtggagac cgcttcttcg acgggcttgg tgatggtctc ctggatctgc tccggcgtgg
     3901 cgttcgggta gggtacgtga accccgacga aaggaaactt gatatccgga gccatcacca
     3961 gcggaatctt tgtggccgaa accgttccca gcacaatgaa cgacgcaaag atcatccacg
     4021 tcgtcacggg atgccggatc gagaactccg aaagccggag tgccaggttc ttatggtcgt
     4081 cgcccgcagg ttccatattc gctgcctccg gttctattgc gccgtctcgg gcaatccggg
     4141 cgagtgctgc ccggccgtta actgccggtc cgataccgca tgagccgtta cctcatccac
     4201 ggcgtatcgt ttgcggtcga tcatctcgta aagcacgggg atgaagatca acgtcagaag
     4261 cgtcgaaaag gagaggccgc ccataatcgt gatcgccatc ggcgttcgga tctcggcacc
     4321 ttcaccccat ccgagcgcca tcggcaccag ccccagaacg ctggtgagcg tcgtcatcag
     4381 aatgggccgc aggcgcacct cgcccgcctg cttcaacgct tcacgccgcg gcagtccctg
     4441 ttcgcgcaac tggttcgcgt agtcgatgag tacgatggcg ttgttcacta caatgccggc
     4501 caggatgatg agaccgagga acaccatcac gttgaatgtc gtaccggtga gggccagagc
     4561 gaagatgacg ccgatcaccc cgagcggcac gctgaacagg atgatgaacg gatgcacgag
     4621 ggattcaaac tgcgaggcca tcaccaggta gaccagaaaa accgcgagcg ccatggcgag
     4681 caccaggctg cggtaggaag tcctcaattc ctcgttctgt ccgccgatgg tgaccgtcgt
     4741 gtcggcagga agcgacgcac gaagacgttg aagctcggcc tcgatctcct cggtagcgct
     4801 gttcagcgcg cgcccgctca ggtttgccga aacaagggcc gcgcgttgcg accggatgcg
     4861 gcgcacttca ctcggcccgc gctgcacaga tacatcggcc accgcaccca gacgaatcgg
     4921 tatcgtgcgc gcttgaacta cggcgccgcc cgctgcccct ccagcctgtt gccgcgtaac
     4981 aggcgcgggt accgcgatgg agccaccgtt tgtatttctg cgggtctccg tcagcggcgt
     5041 ctcctccaaa acccgcgcgt tcccgcccat gttgatggtg aggttcctga tattctccac
     5101 ccgattgcga tcggattcgt ccgcccgcac cagaatctcg atctgcttgt cgccttcccg
     5161 gaagcggctc gcaacatcgc cgcgcacttt gttccgcacc gtggcggcga tttcaccttc
     5221 gtcgagaccg agccgtgcca gccggtcccg gtcgaacctc acctggattt cgggattacc
     5281 cagctcggtg ctggtcctca cgtcgctcaa tccccgcata gtctccagcc tccgggccac
     5341 cagatccgcc gtcgtgcgca gattgcccag atcctgcgaa tagatctcca cctcgatcgg
     5401 cgttttgaaa ctgaagaacg tagggcggct gaaggtgaac gacacttccg gcagttgccg
     5461 cagctccgcg cgaatgcggt tgatggtttg ctcctccagg cgtttgtcgt tcttatcgct
     5521 catgacgacg tacaactggc ccacgttctc ctctcgtgtt tcgcgcgcga actgattacg
     5581 gttgctgccc ccgacgctgg aaaatacggt gtggacgccg ggcaggacgg cgatgcgctt
     5641 ctccattccg ttcacaatcc ggtccgtgtg ttccagcgcg cgtccttccg gcagacggag
     5701 ctcaaacacg aactctccct gggtgagcgg cggaatcagc tcggccccga taaatggaac
     5761 cagaaccgcc gcgacggcag caaggccaag cgcgaagccg atgaccgtaa ttttgtgatt
     5821 cagagcccaa tccagcagac cgggataacg gccgctggcc cacttgtatc cgctatcgaa
     5881 gcggtcgaga accggattca aaggccacag cacaagacgg cccagcatcc gtacgaggcg
     5941 ccggaggtcc gctacgacga tgcgcaccat gtcagtgaag acgaacgcac ccaccgcgcg
     6001 cgcctttgtc tccagaaacc aggccggccg ctggagggca cgaacccaac gccgtgagct
     6061 cggctcgcgc cgcggcgcaa cgttgtcctg cggttcgggg gcatgcttgt tcacctgaag
     6121 ggccaggagc atagggatca gcgtcaaccc gataatcagg gaaacgatct gcgagtaagt
     6181 gatggtgagt gcctggtctt tgaagagctg cccggcgatg ccttcgatga agatcagcgg
     6241 cagaaagacc gccactgtcg tcatcgtgga tgccgttacc gccatcgcca cttctttcgt
     6301 tccacggaac accgcgtcgt cgagcttggt gccctgacgc ttgtggcgga acaccgattc
     6361 cagcacgaca atcgagttat ccaccagcat tcccacgccc agggcgacgc cgccgagcga
     6421 catgagattc agcgtgatgc cagtctgata catcagggcg aaggttgcca tgatcgaaat
     6481 cggggtcgta attccgatca gcagcgtact gcgcatgtcc cgaagaaaca ggtacaaaac
     6541 cagaatggcg agcagaccgc cgaggatggc cgaagagtga atatcctcga tggcatcgct
     6601 gataaagtca gcctggttga aaaccacctg gtattcgacc ccgcgcggaa tcaggttcgc
     6661 ccgcttcagc gcgtcgaggc ggtcctgaac ggcgcgcgcc accgtgacgg tgttcgcgtc
     6721 gccctccttg taaatcgcca tctccacgct ttcttttccg ttgatgcggg cgatgatctc
     6781 ccgatccttg gcgccccgcc agaccttggc cacgtcgccg agcaccactt ttcttccggc
     6841 ctcgtcgcgt acgacaattc cgcggatctc ttcgacgtcc ctgaactggt tcagcatccg
     6901 gaccatgtag ttcgcgtcca ggtcgtatag gctgcctgaa gcctggttca gattttcctg
     6961 cgccagaatg cgagtgactt ccgtaatagg aatccccagt tcggccaacc tcttttcgtc
     7021 gatctcgata cggatctgct cttcgagccc gccgatcact ttgatcgcgg ccactccgct
     7081 ggtggattcc aactggcgct tcagctcgcg ctccgccgca tagcgcagcc ggctcaaact
     7141 catgcccgtg ccggaaagct ggagccgcat aatggggtcg taggaaggat cgaaccgcag
     7201 gattacgggc cgtccggcat cgcgaggcag cgtgacgaaa tcgagtttct ctctggtatc
     7261 cagggccgcc agatccatat tggtgtccca gccgaactcg agaaccactt cggactggcc
     7321 ggggcgcgaa acagagctca accgcgtcaa acccggcagc accccaacgg actcttcgat
     7381 cggacgcgtg atgagcgctt ccacctcttc gggagccgcg tcttcatagt cggtctgtat
     7441 cgtcagcgac gggtaggaga tgtccggcag caggttcaag gcgagccggt caagcgagac
     7501 ccacccgaag agaatcaccg cgacgacaaa catggatacc gtaacaggac gatgtatggc
     7561 gcttctgatg acgttcatga aacctcacgc gcggcaatcc gcctgaacgt tctgccgcct
     7621 cttcagcttt ggatggatct gattttggag ccctctttga gtgcgccgtg gcctgctacc
     7681 accacctgtt cgcccgctcg aataccgctg cggatctcga ccacgccctc actgctgtag
     7741 ccgacgtcga ctttcgtgcg tttcgccgta gtcccattgg ccacgaacac gtaattctgc
     7801 ccgtcttctt cgatgacggc gcgtttggga acgaggatgg cgcctggctt cgtgtcggtc
     7861 cggatgtcca cccgaacgaa cgcgccgggg cggaaattcg ccgcgaccgg tttcataacg
     7921 atggtcacct tcacggtccc ggtggactgg tcgacaatgg ggctgaggcg ctccacctgg
     7981 cccttcagtt ccgcccccgc atcggcgcct aaacgaacca gcgccggctg tcccggatgc
     8041 acctcttgcg catcgagctc ggacaggtgc acatcgacga acagcggcga aaaggagccg
     8101 atggtgaaga gcgcatccag gttccttatc cgctggccca gctcgaccgt ccggtgagtg
     8161 atatagccgg agaacggagc gcggattcgt gttcgggaca gttccagctc ggcctgtgcg
     8221 atttccgctt cgagctccgc cacccggtgc gtgaactcgc gaatttcctg cgtggaaacg
     8281 cgttcatcgt gctcgagttc atccagccgg tacttccgct gcgcgacgtc caattcgctc
     8341 gcaaggccct ctttgtaggc tttctgataa cgctccagct ccgttcgggc attcaggatt
     8401 tgaaccgcgg ccttctcctg gcgcgtgcgc gctttttcca tttgcagctt cgcctgcgcc
     8461 agccgttcgc gcgcgagctc cagccggatt ttgagctgag cgtcgtcgag ctcacacaga
     8521 acctggccct ccttgatcag gtccccctct tccgcca
//
LOCUS       DASTMK010000366         8946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_433939, whole genome shotgun sequence.
ACCESSION   DASTMK010000366 DASTMK010000000
VERSION     DASTMK010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433939"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFQ79_15980"
     CDS             complement(<1..501)
                     /locus_tag="VFQ79_15980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU0141222.1"
                     /translation="MASRRRFLQTAAALGAASSLAAAPQYQLGCETLPYRSFPLERAL
                     EGIRKAGYRYVMPYHMHAGKVVFGPSLTSAERASVRRKFEDQDLQLFMVFIGLDKDSR
                     LPEGLKGYLAELDFFAEMGIRTAVGVGPWYFTRFPNVPKRDRDWQKEVDEYYRALEPA
                     VRHAEKL"
     gene            complement(513..1730)
                     /locus_tag="VFQ79_15985"
     CDS             complement(513..1730)
                     /locus_tag="VFQ79_15985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141223.1"
                     /translation="MSESTWNRRRFLAAASVSAAAAGRAIGANDRLSVAIVGCGGRGL
                     LKEVLEFSTATNSEVTVVCDTWRQQREKAAAAVAEATGKQPKTAVRYQDVLAMKDVDA
                     VVIGTPDHQHCTMLRDSVRAGKDTYIEKPLAMNMKELLEAVDAVKQSGRIVQVGTQVR
                     SFSSSAAGRAFVRSGGLGSIFKIEQSRNAYRPYWHSYAQRPVEQADVDWESFLMHVKH
                     RPWNADQYAGWYGYRDFSLGPQTGLLSHFIDLVHFVTGAKFPKKATAMGGTFRWKDAR
                     TAPDSFEALFEYEEGFLVRYNTSFGTSSNNFLKFFGTRGVMDATQWARPWVLTGQPGE
                     PDSLPQGATIPEAESTPHMKNWLECIRNRKEPNAPIEAGFQHAVACLMAEESWVRGHR
                     VTFDPARRKIMAG"
     gene            complement(1832..3277)
                     /locus_tag="VFQ79_15990"
     CDS             complement(1832..3277)
                     /locus_tag="VFQ79_15990"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEU0141224.1"
                     /translation="MSIRLSTGIEIPIEMHKVRIVQKVRLLPAKERLRAIEEAGYNTF
                     LMRSRDLFLDMLTDSGTNAMTDNQQAAMMQADDAYAGSESFYRLEDAVREIFGYRYVL
                     PAHQGRAAEHLLAKTFLKPGNVVPMNYHFTTTKAHFELAGASVRELFAAEALNTESTH
                     PFKGNIDVTRLKETIDDAGPRNIPFVRMEATTNLLGGQPFSLANLREIKALIEPMGIP
                     LVVDGSLISENAYFIRQREEGYAGASIKDIILEIMSVADLCYLSGRKSCSVRGGLIAT
                     NRRDLHEAIRPWLPVYEGFFTYGGMSSKEVEAMAVGMREMTELDVAGSSADAIKYFAN
                     RLIQLGLPVVTPPGGLACHLDATRFIPHVPQEDYPAGALAAAVYIASGIRGMERGTIS
                     MDRDAEGRDVLSDLELVRLAVPRRVFTMSHFEYAADRLKWLHNHRDLIGALGFCQEPP
                     VLRFFAGKLKAKSDWPEQLASAFEKDFGPHC"
     gene            3431..3829
                     /locus_tag="VFQ79_15995"
     CDS             3431..3829
                     /locus_tag="VFQ79_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEU0141225.1"
                     /translation="MSITLDVARKIIAAAEKKAAEIKQPMNIAVVDEGGNLVAHVRMD
                     GAWIGSIDISINTAYTSRAFAISTKDLASHSQSGGQFFGIHVSNGGRIMIFAGGIPLK
                     SGGKVVGAIGVSGGSGEQDHAVAEAGAAAL"
     gene            complement(3843..5030)
                     /locus_tag="VFQ79_16000"
     CDS             complement(3843..5030)
                     /locus_tag="VFQ79_16000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0141226.1"
                     /translation="MATSDRKIHRRLLSVAAYAGMFVFGIVMALLGTILPVLSARLEF
                     QVSSIGTLFLVMNFAMLASSLLLGVAMDRFGMKPPLTAGPLLVAVSLVMILRADIFVE
                     LIPAVVLLGIGGGALNGATNTLVADLHDEPKRKSSALNVLGVFFGFGALFLPFTVGVL
                     LAALGLERLLLFAALLCMAAGVFSVALRFPPPKQRQRVPFAVMPRFLRSPLVLFAAAL
                     LFFQSGVEFTLGGYIATYLTNTMGFAVSSASFVLAGYWAALMVSRIALSRMLLGGDPH
                     RFVTLCALGACAGAVLTVVASSPALAAAAIVFTGLALAGIYPTVLGIAGAEFREHSGT
                     VFGLLFTVALTGGMTLPWVAGQAAQAAGLRWVFVIVAVSFGAIFVLGRLMARPSTDTA
                     AVR"
     gene            complement(5017..5790)
                     /locus_tag="VFQ79_16005"
     CDS             complement(5017..5790)
                     /locus_tag="VFQ79_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0141227.1"
                     /translation="MSAKNYFQRISEVLGSINEREMKKLEEAGEMLAAAIQAGKRVYL
                     FGSGHSVIPVMDVFPRYGSFVGFFPLYDPRLMWHNVVGPGGARELLWLERREGYIEHF
                     LQSYAFDAGDCMIVFSHGGLNAAPIEAALYAKERGLKVITVSSLANAEIAAATHSSGK
                     KLSDVADIALDNCVEPEDAQVDIGRREKVAAGSTIAAVFLAMALVAETGARLAANGTV
                     PPTFVSPNVSGVEKDHNFRVFDAFTGKLFERSPVPHGNV"
     gene            complement(5805..6677)
                     /locus_tag="VFQ79_16010"
     CDS             complement(5805..6677)
                     /locus_tag="VFQ79_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0141228.1"
                     /translation="MNSVIAIDLGGTKTAMAVVDEAGYVHHKQKFPAARTLEGTIDQV
                     VAYTSDAGVAQVGMIVPGIYHPRNGTAWAPNLWGHDYVPLRDALQDRLSLRTAIASDR
                     TGYVLGEQWLGVARGLSDVIFVAVGTGIGVGILAGGHIVEGAHGIAGSAGWMAIESAW
                     KPEYAEMGCWESEAAGPALARRAGMLSAETVTAAARTGDQHAVTAIAETAEYLAKGIA
                     NLISVLDPELVVLGGGLMQAADLLLEPVRAAVPRWAQPVAAKLARIERTWLGEDAGLL
                     GAARLALLHTCRQD"
     gene            complement(6674..>8946)
                     /locus_tag="VFQ79_16015"
     CDS             complement(6674..>8946)
                     /locus_tag="VFQ79_16015"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU0141229.1"
                     /translation="TGETIARTAMPKVFLFLLCAPVFATEFFPVAVWYGGGKARAPML
                     ESGARGKKETWRKDIRTIKSLGFNTVRTWIDWATGEPRQDQYNFENLDVILELAEEEG
                     LKVFLQVYMDSAPHWVGRKFPDSYFVSSNGAVVEPESSPGYCMDHPGVRQRDLAFYAA
                     LAGRARRSKAFVGWDLWSEPHVINWANPTYIPNPEFCFCKNTIAKFRSWLQKKYGSIG
                     KLNEAWYRRYEDWDDVVPGRMSTILSYTDYIDWKTFIAGKLGDDLAARYAAVKDAAPQ
                     TIVTSHAAGVGLFVSPHHWEGQSDDWTMAQKVDYYGTSFYPKHSAFVDRDVPWRAALL
                     DFTRSFGFAGGGRGFYIGELQGGFGTIALNVSPTVTPEDIRVWAWSALARGAKGVNFY
                     AWYPMSSGYESGGFGLIELDGTVTERARVAGSISRVVDQNQKLFLDARPPKAQVAVVY
                     NPLSHFVGGRQRATAYGGPQGEVASIERDSLLGIHRALFPHNVPLDYIHINHLSPDLL
                     AQYKLVVLPYPLMLPEASAKHFKEYVRGGGALVAEARLGWNNERGYAAERIPGMGLWE
                     VMGCRETAVQTGVKGRTALRWTSPDLPGLKPGEMLAARWYEETIEPLSESARVVAEFA
                     SGAPAAVMSRYGKGKTLMLGSYLSAAYQSTPTPEVERFYTGLLEWADVELPVKTSKGG
                     IEVRTLETGPDTLLFVFGHGKAPTDAIVDVRTAALTAFDLVAQKPVPAERAGDMLRLR
                     KRVEPSEVWVLRLSLQ"
BASE COUNT         1800 a         2792 c         2613 g         1741 t
ORIGIN      
        1 cagtttctcg gcatggcgca cggcaggttc gagcgcgcgg tagtattcgt ccacttcttt
       61 ttgccagtcg cggtcgcgct tgggcacgtt ggggaagcgt gtaaaatacc acgggcccac
      121 accgacggca gtacgaattc ccatctcagc gaagaaatcg agctccgcca ggtagccttt
      181 cagaccttcc gggagcctgc tgtctttgtc gagcccgatg aagaccataa aaagctgaag
      241 atcctggtcc tcaaacttgc gccgcacgct cgcacgttcg gcggaggtaa gagatggtcc
      301 aaaaaccact ttcccagcgt gcatgtggta gggcatcacg taacggtaac ccgctttacg
      361 aatgccttcc agcgcgcgct cgagaggaaa ggagcggtag ggcagcgtct cgcaaccgag
      421 ttgatattgc ggcgcggcgg caagcgaact cgccgcccca agcgccgccg cagtctgtaa
      481 aaatcttctt cgggaagcca tcgattctct ccttagcctg ccataatctt gcgcctcgcc
      541 gggtcgaatg tgacgcgatg cccccgcacc caggactcct cggccatcag gcacgccacg
      601 gcgtgctgaa agccggcctc aatcggcgcg ttcggctctt tccggttgcg tatacattcg
      661 agccagttct tcatgtgcgg cgtactttcg gcttcgggaa tggtggcgcc ctgaggcaga
      721 ctatccggtt cgccgggctg ccccgtcagc acccagggac gcgcccattg tgtcgcgtcc
      781 atcacacccc gcgtgccgaa aaacttcagg aagttgttgc tgctggtacc gaagctggtg
      841 ttgtagcgga caaggaatcc ctcttcgtat tcgaacagag cttcgaaaga atcgggcgct
      901 gtgcgggcgt ctttccatcg aaatgtgccg cccatggctg tcgccttttt tggaaacttc
      961 gcgccggtta caaaatgtac gaggtcgatg aagtggctta gcagcccggt ttgcggacca
     1021 agcgaaaaat cgcgatagcc gtaccagccc gcgtactggt ctgcgttcca ggggcgatgt
     1081 ttcacgtgca tgagaaacga ttcccagtca acgtcagcct gctcgaccgg ccgctgcgca
     1141 tagctgtgcc agtaggggcg gtaggcgttg cgtgattgtt caatcttgaa aatgcttccc
     1201 aatcccccgg atcggacgaa cgcccggccc gccgcggaac tggagaagct gcgcacttgc
     1261 gtgcccacct gtacgatgcg cccggactgc ttcaccgcat ccacggcctc cagcagttcc
     1321 ttcatgttca tcgccagagg tttttcgata tacgtgtcct tgcccgcgcg cacgctatcg
     1381 cggagcatcg tgcaatgctg gtgatccggc gttccgatca ccaccgcatc cacgtctttc
     1441 atcgccagga cgtcctggta tcgcacggcc gtcttgggtt gtttgccggt tgcctctgct
     1501 accgcggcgg cggccttctc ccgctgctgc cgccaggtgt cgcaaacgac ggttacttcc
     1561 gaattggtgg ccgtcgaaaa ttccagaact tctttgagca ggccgcgtcc tccgcagcct
     1621 acgatcgcga ccgacagccg gtcgttcgcg ccaatggccc tgcctgccgc ggcggcgcta
     1681 acggatgccg cggcgaggaa ccggcggcgg ttccaggttg attcggacat atgaagctcc
     1741 tatgtcctta cagtctattg cgagctcgac gcagttgttg cggacgacgc ctcttatttg
     1801 tccaaaattc tggacgtgcg gctgtgctgc ctcagcaatg cgggccgaag tctttctcga
     1861 acgccgatgc caattgctcc ggccaatcgg atttcgcttt gagctttccg gcgaagaagc
     1921 gcaagacagg cggttcctgg cagaagccga gggccccgat caaatcccgg tggttgtgaa
     1981 gccatttcag acggtccgcg gcgtattcga aatgggacat cgtgaacacg cgccggggaa
     2041 cggcgagccg tacgagttcg agatccgaga gaacatcgcg tccttcggcg tcacggtcca
     2101 tcgagatcgt tccgcgctcc attccgcgga ttcccgaggc gatgtacacc gccgcggcca
     2161 gcgctccggc gggataatct tcctgcggta cgtgcggaat gaatcgcgtg gcgtccaggt
     2221 ggcacgccag gccgccggga ggcgtcacta ccggcagtcc gagttgaatc aggcggttgg
     2281 cgaagtactt gatggcgtcc gccgacgagc ctgccacgtc gagttccgtc atctcgcgca
     2341 tgccgacagc catggcctcc acttctttgg aagacatgcc gccgtaagtg aaaaagccct
     2401 cgtagaccgg cagccatggg cgtatcgctt cgtgcaggtc tctgcgattg gtagcgatga
     2461 gtccgccccg gacgctacaa ctttttcgtc cggacaggta acagagatcc gccacggaca
     2521 tgatctccag gatgatgtcc ttgatggaag cgccggcgta tccctcctcc cgctggcgaa
     2581 tgaagtaggc gttctcggag atcaggctgc catccaccac cagcggaata cccatcggct
     2641 caatcaacgc ctttatctcg cgcagatttg cgagggaaaa gggctggccg cccagcaggt
     2701 tggtggtcgc ctccatccgg acaaacggta tattccgggg gccggcatcg tcgatggttt
     2761 ccttcagccg cgtgacgtcg atattcccct tgaacggatg ggtgctctcg gtgttcaagg
     2821 cctccgccgc gaacagttcc cggacgctcg cgcctgccag ttcaaagtgg gccttggtgg
     2881 tggtgaagtg gtagttcatg ggcaccacat tgcccggttt caggaacgtt ttcgccagca
     2941 ggtgttcggc ggcccggccc tgatgcgcgg gcagaacata ccggtagccg aagatttcgc
     3001 gcacggcgtc ttccagcctg taaaagctct cggagccggc gtacgcatcg tcggcctgca
     3061 tcatggcggc ctgctgattg tccgtcatgg cattggtgcc gctgtcggtc agcatgtcca
     3121 ggaaaagatc gcgcgaacgc atgaggaacg tgttgtaccc cgcttcctct atcgcccgaa
     3181 ggcgttcctt ggcgggcagc aggcgcacct tttgcacaat tcgaactttg tgcatttcga
     3241 tcggaatctc gataccggta gaaagacgaa tactcatgat tattcggatg atatcatccg
     3301 gaagtggtaa atgggagtaa acagtcagga tttccgcttg ctggcgcgcg cggctcggtt
     3361 ccgggccgtg acctgcaact ctatcatccg cggcaaagca tgcgctcccg ttttcatcca
     3421 caatagagca atgtccatca ctcttgatgt cgctcgaaag atcattgccg ccgccgaaaa
     3481 gaaagcggcg gaaatcaagc agcccatgaa catcgccgtt gtggacgagg ggggcaacct
     3541 ggtggcgcac gtgcgcatgg atggcgcctg gatcggcagt atcgatatct ccatcaacac
     3601 ggcgtacacc tcgcgcgcgt tcgctatctc taccaaagac ctggcttcgc attctcaatc
     3661 cggcggacag tttttcggga tacacgtctc caacggcggc cggatcatga ttttcgccgg
     3721 cggcattccg ctcaaaagtg gcggcaaagt ggtcggcgct atcggtgtca gcggcggatc
     3781 cggggaacag gatcacgccg tagcggaggc gggggctgcc gcgctctgag ccgggatatc
     3841 ccttaacgaa ccgcagccgt atctgtgctc gggcgcgcca tcaaccgccc gagcacgaat
     3901 attgcaccga acgatacggc tacgatgacg aatacccaac gcaggccagc cgcttgggcc
     3961 gcttgtcccg ccacccatgg cagggtcatg cctccggtca gtgcgaccgt gaaaagcaga
     4021 ccgaacacag ttccggagtg ttcccggaat tccgcgccgg caattccgag aacggtcggg
     4081 tagatcccgg cgagcgccag tccggtgaaa acaattgcgg cggcagcgag tgccggcgag
     4141 gacgcaacca ccgtcaatac cgcccccgcg cacgcgccca acgcacagag ggtgacgaac
     4201 cggtgcggat cgccgccgag cagcatgcgg ctgagcgcga tgcgtgaaac catcaacgcc
     4261 gcccaatagc ctgccagaac gaaggatgcg ctcgataccg cgaaccccat ggtgttcgtg
     4321 aggtacgtgg caatgtagcc gccaagtgta aactccactc cggactggaa aaacaacagc
     4381 gccgccgcaa acaggaccag tggtgaacgg agaaagcggg gcattaccgc gaagggtacc
     4441 cgctgccgct gcttcggggg cggaaaccgc agcgctacgg aaaatacccc cgcggccatg
     4501 catagcaggg cagcgaataa cagcagacgc tccagaccta gcgccgcaag cagtaccccg
     4561 acggtaaaag gcaggaacaa cgctccgaaa ccaaagaaca cacccaggac gttcagcgcc
     4621 gagcttttac gcttcggctc atcgtgaagg tcggcaacca gtgtgtttgt tgcgccgttt
     4681 agcgcgccgc cgccaatccc cagcagcaca accgcgggga tcaactccac aaaaatatcc
     4741 gcccgcagga tcatcaccag ggataccgcc accagcagtg gaccggctgt gagcgggggt
     4801 ttcatcccga aacggtccat ggccaccccg agcagcaggc tcgaagccag catggcgaag
     4861 ttcattacca ggaacagtgt gccaatgctg gagacctgaa attcgagccg cgcggagagc
     4921 accggcaaaa ttgtgccaag cagcgccatg acaatgccga aaacaaacat gccggcataa
     4981 gcggctacgc tcagcagtct tctgtggatt ttccggtcag acgttgccat gcggaaccgg
     5041 cgaacgttca aacagcttcc cggtgaatgc gtcaaatacc cggaagttgt ggtctttctc
     5101 cactccggaa acattcggcg atacgaatgt cgggggcacg gttccgttgg cggccagccg
     5161 cgcaccggtt tcggccacaa gcgccattgc aaggaacaca gccgctatcg tggagcccgc
     5221 cgctaccttc tcgcgccggc cgatatccac ctgtgcgtcc tcgggctcta cgcagttatc
     5281 gagcgcgatg tcggccacat cggaaagctt cttgccgctc gaatgcgtag cggctgcgat
     5341 ttcggcattg gcgagcgagg aaacggtgat caccttcagt ccccgttcct tcgcgtaaag
     5401 ggcggcttca atgggcgcgg cattcaggcc gccatgtgag aacacgatca tgcagtcgcc
     5461 cgcgtcaaac gcgtagcttt gcaaaaaatg ttcgatgtag ccttcgcggc gctccagcca
     5521 aagcagttct cgcgcgccgc cgggacctac cacgttgtgc cacatgagcc gcggatcgta
     5581 aagcggaaaa aacccgacaa agctcccgta gcggggaaac acatccatga cggggatgac
     5641 ggagtgtccg ctgccaaaca ggtagacgcg ctttcccgct tgaatcgcag cggcaagcat
     5701 ctcgcctgcc tcttcaagtt tcttcatctc acgctcgttg atggagccga gaacctcgct
     5761 gatacgttgg aagtaattct tagcagacat agggtagtcc tgagttaatc ctggcggcag
     5821 gtatgcagca gcgcgagcct tgcggcgccc agcagtcccg catcttcacc caaccaggtc
     5881 ctttcaattc gtgccagttt cgcggccacc ggctgcgccc atcggggcac ggcggcgcga
     5941 acgggttcaa gcagaagatc cgccgcctgc atcagtccac cgcccagcac aaccagttcc
     6001 gggtcgagga cactgatcag gttggctatt cccttcgcaa gatactccgc ggtttcggcg
     6061 atggccgtga cggcgtgctg gtctcctgtg cgcgcggcgg cggtgacggt ttcagcggag
     6121 agcattccgg cgcggcgcgc cagcgcaggg ccggccgcct cggactccca acagcccatt
     6181 tcagcgtatt ccggtttcca cgcgctttcg atcgccatcc atcccgccga tccggcgatt
     6241 ccgtgtgctc cttcaacgat gtggcccccg gcgaggatac caacgccaat acccgtaccc
     6301 accgccacga agatcacgtc gctcagtcca cgtgccacgc cgagccactg ctcgcccagc
     6361 acataacccg tgcggtcgct ggcgatagca gtgcgaaggc tcaggcggtc ctgtagagcg
     6421 tcccgcagcg gaacgtaatc gtgcccccat agattcggcg cccacgccgt tccattgcgc
     6481 gggtgataga tacccggcac aatcatgcct acctgggcta ctccggcgtc cgaggtatag
     6541 gcaacaacct gatcgatcgt gccttcgagg gtgcgggctg cgggaaattt ctgcttgtgg
     6601 tgcacgtagc ccgcttcatc cacaacggcc atagccgttt tcgttccgcc gagatcgatt
     6661 gcgattacgc tattcattgc agagataacc ggagcaccca gacttcggaa ggctccacgc
     6721 gttttcgaag ccggagcatg tctccggcgc gctcggccgg taccggcttc tgcgccacaa
     6781 ggtcaaacgc tgtgagcgcc gccgtccgaa catcgacgat cgcatccgtt ggggctttgc
     6841 catgtccgaa aacgaacagc aacgtatccg gtccggtttc cagtgtgcgg acttcgatgc
     6901 cgcccttcga cgtcttcacc ggcagttcca catccgccca ttcgagcagt ccggtgtaga
     6961 agcgctccac ttcgggcgtt ggcgtcgact ggtatgccgc gctcaaatac gatccgagca
     7021 tcagggtctt tcctttcccg tagcgagaca tcacagccgc cggcgctccg gatgcgaact
     7081 cggccactac ccgtgcgctt tccgagaggg gctcgatggt ttcctcgtac caacgcgccg
     7141 ccagcatctc tcccggtttc aggccgggca agtccggcga tgtccagcgg agagccgtcc
     7201 tcccttttac accggtctgg acggcggttt cgcggcaacc cattacttcc cacagaccca
     7261 tgccgggaat gcgttcggct gcataaccac gctcgttgtt ccagcccagc cgcgcttccg
     7321 cgaccagggc gccaccgccg cggacatact ctttgaaatg tttcgccgat gcctccggca
     7381 acatcagcgg atacggcaac actaccagtt tgtattgcgc cagcaggtcg ggagacaggt
     7441 gattgatatg gatgtagtcg agcggcacat tgtgggggaa cagagcgcgg tggatgccca
     7501 atagggaatc ccgctcaatg ctcgcgacct ctccctgcgg gccgccatag gccgttgccc
     7561 gctgcctgcc tcctacaaaa tgcgacaaag ggttgtagac tacggccact tgcgctttcg
     7621 gcgggcgtgc gtccaggaac agcttctgat tttggtcgac tacacgcgag atggaaccgg
     7681 ctacacgcgc ccgttccgtg acggtcccgt ccagctcgat cagaccgaat ccgccgctct
     7741 catagccgga actcatggga taccacgcgt agaagttcac gcctttggcg ccgcgggcga
     7801 gcgccgacca ggcccatacg cggatatcct ccggtgtgac agtgggactg acattcaatg
     7861 cgatagttcc gaagccgccc tgcaattctc cgatatagaa gccgcgcccg ccgcccgcga
     7921 acccaaaaga gcgcgtgaaa tcgagcaacg cggcacgcca ggggacatcg cgatcgacga
     7981 aagcggaatg cttgggataa aaggatgtgc cgtagtaatc caccttctgc gccatagtcc
     8041 aatcgtcgga ctggccttcc cagtggtgcg gcgaaacgaa caggcccacg cccgccgcgt
     8101 ggctggtgac gatggtctgg ggagcggcat ccttcacggc tgcgtaacgc gcggcgagat
     8161 cgtcgcccag cttgccggcg atgaaagttt tccagtcaat gtagtcggta taggagagga
     8221 ttgtactcat ccgccccggc acaacgtcat cccagtcttc atagcggcga taccaggctt
     8281 cgttcagctt cccgatagag ccgtatttct tttggagcca ggaacggaat ttggcgatgg
     8341 tgttcttgca gaagcagaac tcgggattcg gaatataggt gggattcgcc cagttgataa
     8401 cgtgcggttc actccacagg tcccacccca cgaaggcctt gctgcgccgc gcccggccgg
     8461 ccagggcggc ataaaaagcg aggtctctct gccgaacgcc aggatgatcc atgcagtaac
     8521 cgggcgagga ctccggctcc acgacggcgc cgttggacga tacgaagtaa gagtcgggaa
     8581 atttcctgcc cacccagtgc ggagccgaat ccatatacac ctgcaggaaa accttgagcc
     8641 cctcttcctc ggccagctcc agaatcacat ccagattctc aaagttgtat tgatcctggc
     8701 gcggctcgcc ggtagcccag tcgatccatg ttcgaaccgt gttgaagccg agcgacttga
     8761 ttgtgcgaat gtccttgcgc catgtctctt ttttcccgcg cgctccggat tcaagcatag
     8821 gagcgcgggc ttttccgcct ccgtaccata cggcgaccgg aaaaaattcc gtggcgaaga
     8881 cgggtgcgca aagcaggaaa aggaaaactt tgggcatcgc cgttcgagct atcgtctcac
     8941 cggttg
//
LOCUS       DASTMK010000367         7015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_178007, whole genome shotgun sequence.
ACCESSION   DASTMK010000367 DASTMK010000000
VERSION     DASTMK010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178007"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3122)
                     /locus_tag="VFQ79_16020"
     CDS             complement(<1..3122)
                     /locus_tag="VFQ79_16020"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0141230.1"
                     /translation="MSARIVTACLLTAGVCFAQGTGTITGNVTDPSGLAAAGATVTAL
                     LEERGTTRTISVDDRGVYVLPLLPVGTYTIKVEAQGFKTFSQSGVTLTLNQNVRVDAR
                     LEVGAVSEAISITAEAPLVDSRSAQLGTLVDSRRVTELPTNGRNVISLAVLLPGATSI
                     SAPQTFTGDRSGPTVSMSGSRGNQNAFLFDGQHFNAVFRNTGLNYPPPDALQEVKVLT
                     NNFSAEYGRNAGAVFNIVTRSGTNEIHGSLWEFHRNHALNARNFFAPSEKPKLIQNQF
                     GAAAGGPIRRDRLFVFGSYEGLRIRPASLSTSAFPLTDAERAGDFSALGTAIRDPLTG
                     EPFPGNRIPANRVDPVAKALISPNLMPLPNRPDGQLVTTAQEPQSNNTYLVRLDYNMS
                     RHTIDARYNLNRAVEADSAGQIPTYLPLSRTADVHSVALGDTFPILPTLLSQTRLSFN
                     RVAATILNLNPIHMTDLGGNFPVLGPKIPPAIEISGRVSMGNGSTVDAINVNESYQLD
                     QILSWNKSSHTIKAGAQLLRLRYLNRSYFLIMGVYAFSGQITGNAAADFLIGRPSNLQ
                     VASPVLEQAGRQFNAYFFVQDDWRVLPRLVLNLGLRYEVPVPWVHPQDFWGTLRPGQQ
                     SQVIKTAPVGLVFPGDAGVPRGLIQTDKNNFAPRFGFAWDIFGNGRTSLRGGYGLFYE
                     TLNADMIQNTMQPYRYSFTINQPYSLSDPLRGQPEIPLTVNLLNPVFTGTPEVFYPDP
                     SLVSPYVQHFNLALQRQVSGDLAVQVAYVGKVGRKLLMGLSDNPAIYGPGATLANINQ
                     RRILSGFGNNSIISSRANSSYNALQVEVTKRYGRGFSLQGAYAFSRSLDMASGIALGA
                     AVPNVFDLSTQWGLSDFHAKHVASLSWIWDVPVTGSSSAFVKTVADGWQVNGLITAKS
                     GSPIDARTGADNALSGTPNQRPDVNGDPTLTGDRPKADRINAWFDRTVFGTPMTGTFG
                     NAGRNVLIGPGDFGANVGLFKNFAVPGRERMRVQFRSEFFNLLNWVNLGNPNATLTSG
                     TRMGRITSAGS"
     gene            complement(3119..6169)
                     /locus_tag="VFQ79_16025"
     CDS             complement(3119..6169)
                     /locus_tag="VFQ79_16025"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU0141231.1"
                     /translation="MSRSPGFVLLLLLCATLSATQIAENGRARAVIVAGDHQAAAELI
                     TYLGRVTGADLATGAPGGDRILVGGSVCAPEIRQLIEQMGRDGYLIKTQSDGALCLAG
                     SGSDGTAFAVYRFLEEFAGVRWLWPGELGEVVPRRPTLGIGRVSIREEPAFVWRDLGP
                     GGALWGPLDKLAAERKLGVSEEHQKTQKLWERRNRFGGERIYGGHAFGEILPPAKLGP
                     IHPEYFALVDGKRDWQYFNGKHRSQPCTSNPDVIRLTTEYARDFFGRHPEYDAFSISL
                     NDGRGFCECDNCRRLDSGQTQVEAADPETGRTARLPVITDRIVAFANQVAEGLAKTHP
                     NKKLILFAYGMYKEPPQRVKVHPNVIIQYTFHASRLGDSRAERDQLGETGAWNGAAKY
                     LGIYEYFVQGNSPDIPRLIPELIARSVRRLYGQGYRYYQTQAGNGYAVNGLNYYILGR
                     LLWNPDADVRAIEADYIRSGFGKAALPVKRYFDRLESRWKELGGKNVGMDTATTASYR
                     ALAEAFPRAWRDACRRDLAEAASVASDMERRRVAFLQSGFDYVDMTLEAVEKTLPLLE
                     SGWKLGRTVAAPANAKMQDFGIALAAWERRHRYVEDHKEDYTLSYLWVRYNDHNRTFN
                     PLAGMRALAAAKSDPEELRSKIRETLFVPDPLPALEAETHGSFEPEPGIIAERVSYAT
                     QYGMRVPAILYLPKQRSSKIPALIIVNGHGGDKHSWYAAYSGVLYARGGAAVLTYDPI
                     GEGERNAEKKSGTRAHDTLERDRILGRRLGGLMMTDVMQAVSYLRSRPEVDPERIAAA
                     GYSMGSFVLSLACATETRLKACVLAGGGNLDGFGEYWDSSKPMCQAYPYQSLEFLGDR
                     AAIIYRLHAQRGPTLAINGLRDSIMQPERRNPVEFFKQLQARVAMPGRVFDTEYIEEG
                     GHRPYFVTRKAALWLERNLDFPNWDESRIRSMPESHILTWAREQRAAMDKSYATELSE
                     GGTKALGEGVPGLTREQLTVFPGHVWARNRNQIILEGWVQRTKQ"
     gene            complement(6476..6550)
                     /locus_tag="VFQ79_16030"
     tRNA            complement(6476..6550)
                     /locus_tag="VFQ79_16030"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6515..6517),aa:Glu,seq:ctc)
     gene            6640..6969
                     /locus_tag="VFQ79_16035"
     CDS             6640..6969
                     /locus_tag="VFQ79_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU0141232.1"
                     /translation="MGARKHSGVSEAGERKAAAVDPAIKAGNLRRLRRIEGQVRGLQR
                     MIEEDRYCADVMVQISAVQEALRTVGRALMRNHLKHCATHAIRGTPEQAHAMYDELVD
                     LIYKHSR"
BASE COUNT         1337 a         2185 c         2068 g         1425 t
ORIGIN      
        1 gagcccgccg acgtgatccg gcccatgcgc gtccctgaag tgagcgtcgc attgggattg
       61 ccaaggttca cccagttcaa caggttgaag aactccgagc ggaactggac ccgcatgcgc
      121 tcgcgacctg gcacggcgaa gttcttgaac aaccccacat tcgcaccgaa gtcgcctggt
      181 ccgatcagga cgttccggcc cgcattgccg aacgtgccgg tcatgggagt cccgaaaacc
      241 gttcggtcga accaggcatt gatacggtcg gctttgggcc ggtctccggt aagggttggg
      301 tctccgttca catccgggcg ctggtttggt gtgccgctca gcgcgttgtc cgccccggtc
      361 cgcgcatcga ttggagatcc ggactttgcc gtgatcagac cgttcacctg ccagccgtcc
      421 gccaccgtct tcacgaaagc actagaggaa ccggtgaccg gcacatccca gatccacgag
      481 agggatgcga cgtgcttggc gtggaagtca gacagtcccc attgcgtgct taagtcgaac
      541 acgttgggga cagcggcccc cagcgctatg ccggaagcca tatcgagcga tctcgagaat
      601 gcgtaggcgc cctgcagcga aaatcccctg ccgtagcgct tggtcacttc cacctggagc
      661 gcgttgtagc tggaattagc tcgcgagctg atgatactgt tgtttccgaa cccggacagg
      721 atgcgccgct gattgatgtt ggccagcgtt gctccgggac cgtagatcgc cgggttgtcg
      781 gacaggccca tcagcagctt gcgtccgact ttgccaacat aggccacttg gacggctagg
      841 tccccggata cctgccgttg cagcgccagg ttgaagtgct gcacgtacgg gctgactagc
      901 gacggatcgg ggtaaaacac ctccggagtc cctgtgaaca cgggattcag cagattcacc
      961 gtgagaggaa tctccggttg cccgcgcaac ggatcgctca gactgtacgg ctggttgatg
     1021 gtgaacgaat accggtaggg ctgcatagtg ttctggatca tgtccgcatt aagagtctcg
     1081 taaaacaggc cgtaaccgcc gcgcagcgag gtgcggccgt tgccgaagat gtcccacgcg
     1141 aatccgaagc gtggagcgaa gttgttcttg tcggtctgga tgaggccgcg cgggacccca
     1201 gcgtctcccg ggaagaccaa ccccacaggc gccgttttga tcacttgcga ttgctgtccg
     1261 ggccgcagcg ttccccagaa atcctgcggg tgcacccacg gcacaggcac ttcataacgc
     1321 agccccaggt tcagaaccag ccgtggcagc acgcgccagt cgtcctgcac aaagaagtac
     1381 gcgttgaact ggcgtcccgc ctgttcgagt accgggctag ctacctgtag gtttgaaggt
     1441 cggccgatca gaaaatcggc ggctgcgttg ccagtgatct ggccgctgaa cgcgtacact
     1501 cccatgatga ggaaatagga gcggttgaga taccgaagcc tcagaagctg cgctcccgct
     1561 ttgatggtgt ggctggattt gttccaactc agtatttgat ccaactgata cgactcattc
     1621 acgttgatgg cgtccaccgt cgaaccgttg cccatcgaca cgcgcccgga gatctcgatg
     1681 gccgggggga tcttcggccc cagcaccggg aagttgccac ccagatcagt catgtggatc
     1741 ggattgagat tgaggatcgt cgccgcgacg cggttgaagc tgagccgcgt ttggctgagc
     1801 agtgtgggca aaatcgggaa cgtatcgcct aaagccacac tgtgcacgtc cgctgtccgg
     1861 ctcaggggca gatacgtagg gatctgtccg gccgaatcgg cctccaccgc gcggttcagg
     1921 ttgtaccgcg cgtcgatggt atggcgactc atgttgtagt ccagccggac gaggtaagtg
     1981 ttgttgcttt gcggctcctg agccgtggtg acgagctgcc cgtcgggacg gttcggcaag
     2041 ggcatcaggt tgggcgaaat cagagccttc gccaccggat cgacgcgatt cgctgggatc
     2101 ctgttgccag gaaacggctc tcctgtcagc ggatcccgaa tcgctgtgcc cagtgccgag
     2161 aaatcgcccg ctcgctccgc atctgtcagc ggaaacgcac tcgtgctcag cgacgctggc
     2221 cggatgcgca ggccttcgta cgaaccgaaa acgaacagcc tgtcgcgccg gatgggaccg
     2281 cccgccgccg cgccgaactg gttctggatc agcttcggct tttccgaggg tgcgaaaaag
     2341 ttgcgcgcgt tcagcgcgtg attgcggtgg aactcccaca ggctgccgtg gatctcgttc
     2401 gtgcccgagc gtgtcacgat gttgaacacc gccccagcgt tgcggccgta ttccgcactg
     2461 aagttgttgg tgagaacctt cacctcctgc agcgcatccg gaggaggata gttcaggccg
     2521 gtgttccgaa acaccgcgtt gaagtgctgc ccgtcgaaaa ggaacgcatt ctggtttccg
     2581 cgtgaaccgg acattgaaac cgtcggcccg ctgcggtcgc cggtgaacgt ctgcggcgcg
     2641 ctgatgctgg tggcgccagg tagcaggacc gccagcgaaa taacatttcg tccgttggtt
     2701 ggcagctctg tgacacggcg tgaatccacc aatgtgccga gttgcgccga acggctgtcc
     2761 actagcggcg cttccgccgt gatgctgatg gcttcgctca ccgcgcccac ttcgagtctc
     2821 gcgtccacgc gcacgttctg gtttaaagtg agcgtcacgc cgctttgcga aaaggtcttg
     2881 aagccctgcg cttccacctt gatggtgtag gtccccacgg gcagtagagg caacacgtaa
     2941 acgccccggt catcaacgga aatcgtgcgt gtcgtgcccc gctcttcgag taacgcagtg
     3001 actgttgcgc ccgccgcagc aagccccgac gggtcggtca cgttgccggt gatcgtaccc
     3061 gttccctgcg cgaagcaaac gcctgccgtc aatagacagg ctgtcacaat tcttgcgctc
     3121 attgtttcgt cctctgtacc catccttcta gaatgatctg gttgcgattt ctggcccaca
     3181 catggcccgg aaaaaccgtt aactgctcgc gcgtgagccc gggtacccct tctcccaacg
     3241 cctttgtgcc cccttcggaa agctccgtgg cgtaggactt gtccatcgcc gcgcgctgct
     3301 ccctggccca cgtcaagatg tggctctctg gcatcgaacg gatgcgcgac tcatcccaat
     3361 ttggaaaatc cagattccgt tccagccaca gagctgcctt cctggtcacg aaatacggcc
     3421 ggtgaccgcc ttcctcgatg tattcggtgt cgaagacgcg gcccggcatc gcgacgcgcg
     3481 cctgcaattg cttaaagaac tccaccggat tgcgccgctc gggctgcatg atgctgtccc
     3541 gcaatccatt gatcgcgagt gtcggaccgc gctgtgcgtg cagccggtag atgatcgccg
     3601 cgcggtctcc aaggaactcc agggattgat atggatacgc ctggcacatt ggcttcgagg
     3661 agtcccagta ctcgccgaat ccgtccaggt tgccgccgcc cgccaggacg catgctttca
     3721 gccgcgtttc ggtagcgcag gccaaactca agacaaacga gcccatcgaa taaccggccg
     3781 ccgcaatgcg ctccggatcc acctccggcc gcgagcgcag gtaggaaacc gcctgcatga
     3841 catcagtcat catcagaccg cctagacgcc ggccgagaat ccggtcgcgc tccaacgtat
     3901 cgtgggcacg tgtaccggac ttcttctccg cgttgcgctc cccctcgccg atgggatcgt
     3961 acgtcagcac cgccgcgccg ccgcgcgcgt acaacacacc cgagtaggcc gcataccaac
     4021 tgtgcttgtc gccgccgtgc ccattgacga tgatcaatgc ggggatcttg ctgctgcgct
     4081 gtttcggaag gtacaagatt gcgggcacgc gcatgccgta ctgggtggca tagctgacac
     4141 gctccgcgat gatgcccggc tccggttcga aactcccgtg tgtttccgcc tcgagagcgg
     4201 gcagcggatc cgggacaaag agcgtctctc ggatcttgct tcttagttcc tccggatcgc
     4261 tcttggcggc tgccaaagcg cgcatccctg ccagcgggtt gaacgtgcgg ttgtggtcgt
     4321 tatagcgcac ccacaggtac gacagggtgt agtcttcttt gtgatcctcg acgtaccgat
     4381 ggcgccgctc ccaggccgcg agcgctatac caaagtcttg catctttgcg ttggcgggtg
     4441 cagcaaccgt gcgcccaagt ttccatcccg attccagcaa tggcagcgtc ttctccaccg
     4501 cctccagcgt catgtccaca tagtcgaagc cgctctgcaa gaacgcgacg cggcgccgct
     4561 ccatgtcgct tgcaacactg gcagcctccg ccaggtcacg cctgcaggcg tcgcgccagg
     4621 cgcgcggaaa cgcctctgcc agcgcgcgat agctggctgt tgtcgccgta tccatcccga
     4681 cgttctttcc gcccagctcc ttccagcgcg actccagccg gtcgaagtag cgtttcaccg
     4741 gcagcgccgc tttcccgaaa ccgctccgga tgtagtcggc ctcgatcgcc cgcacgtcgg
     4801 cgtcagggtt ccataacagc cgcccgagaa tgtagtagtt cagcccattg accgcgtacc
     4861 cgttgcccgc ctgtgtctgg taatagcggt agccttggcc gtaaagcctc cggacactcc
     4921 gcgcaatcag ctccggtatc agccggggga tatcgggact gttgccttgt acgaagtact
     4981 cgtagatgcc gagatacttc gccgctccgt tccacgctcc cgtctcaccc agttgatcgc
     5041 gctccgcccg cgaatccccg agccgcgatg cgtgaaatgt gtactggatg atcacgttcg
     5101 ggtgtacctt gacccgctgc ggcggttcct tgtacattcc gtacgcaaac aggatcagct
     5161 tcttgttcgg atgggtcttc gccaagccct ccgcgacctg gttcgcaaaa gccacgatgc
     5221 gatcggtgat caccggcagc cgggccgtcc tgccagtctc cggatctgcg gcttccacct
     5281 gcgtctgccc gctatcgaga cgccggcagt tgtcgcactc gcagaatccg cggccatcgt
     5341 ttaaggaaat agaaaacgcg tcgtactcgg gatgcctgcc gaaaaagtcc ctcgcgtatt
     5401 cggtggtaag ccggatgacg tccgggttgc tggtgcaagg ttgtgagcgg tgcttgccgt
     5461 tgaaatactg ccagtcgcgc ttcccgtcca ccaacgcgaa atattcggga tgaatgggac
     5521 cgagctttgc aggcggcaga atctcgccga acgcgtgccc cccgtaaatc cgctctccgc
     5581 caaagcggtt gcggcgttcc cacagcttct gggtcttctg gtgttcctcg gaaaccccga
     5641 gtttgcgctc ggcagccaac ttatccagcg gaccccatag cgcgccgcct ggaccgagat
     5701 cgcgccaaac gaacgccggc tcctcccgaa tagagaccct gccaataccg agagtgggcc
     5761 ggcgtggcac cacttctcca agttcaccgg gccaaagcca tcgcactccg gcaaattcct
     5821 cgagaaatcg gtacaccgca aaggctgtgc cgtccgagcc gctgcccgca aggcacaagg
     5881 cgccgtcgga ttgtgtcttg atcaggtatc cgtcccggcc catctgctcg atcaactgcc
     5941 ggatctccgg ggcgcaaacg ctgccaccca ccaggatgcg gtcgccgccc ggcgcaccag
     6001 tagcgaggtc cgcccccgtg acgcgcccta gataggtgat caattcggcc gcggcttgat
     6061 ggtctccggc cacaatcacg gcccgcgcgc gtccgttctc ggcgatttgt gtggcggaca
     6121 gtgtggcgca aaggaggagt agtagaacga acccaggtga cctgctcaca agcacatcct
     6181 ttcgtgcgga cagcgtggct ggcgcgatct tccagtatgc gatcaaatat attatatgcg
     6241 gccaatatca tagataaaga cctttttatt ctagatatgc agttggcaaa agccggaatg
     6301 tggcttttac ctcggcttac cgcctgtgtg cttcgcagtc cggctcttga ttttcccgcg
     6361 agtcagcacg gaacggcttg gggcactctt cacctcgcgt cactgccggc atctacgcgc
     6421 atgcgataac cggccgagat cgtcttgtaa gtttttttga tgaatggaag ttttttggta
     6481 gcgctaacgg gaatcgaacc cgtatttgag ccttgagagg gctccgtcct aaccgttaga
     6541 cgatagcgcc agaacgtaac atcagtatac caaaggtctg acttgcgtct cttgacaata
     6601 cccccatcgg gtatcttaag atacccgtat agggtattta tgggcgcaag aaagcattca
     6661 ggagtgtcgg aggctggaga gcgcaaagca gccgccgtcg atcccgcgat taaagccggc
     6721 aatctgcggc gtttgcgccg cattgagggc caggtgcgcg gccttcagag aatgatcgaa
     6781 gaggaccggt attgcgcgga tgtcatggtg cagatatcgg ccgtgcagga agccctcaga
     6841 acggttggcc gggccttgat gcgcaaccac cttaaacatt gcgcaacgca tgcgatcaga
     6901 ggtacgccag agcaggccca cgccatgtac gacgagctgg tggatctgat ctacaaacac
     6961 tcgcgttgaa cgccatcagc gaaaggaggt gatcatccat ggagaaagat cccgt
//
LOCUS       DASTMK010000368         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_114048, whole genome shotgun sequence.
ACCESSION   DASTMK010000368 DASTMK010000000
VERSION     DASTMK010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114048"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFQ79_16040"
     CDS             <1..858
                     /locus_tag="VFQ79_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0141233.1"
                     /translation="LLKVAGLALLISSAFLYRGPVALEWASPASVLSLRSVAIAMLGC
                     FLAYDGWQYIAFVAGEVRNPQRNLPLALGLGTGAVIILYLLANLAYMRVLPLSQIAST
                     ERVAAVVSEQTMGSAGAALVALTIMLSCAGAANGAILSSPRIYFAQARDGLFFQRFAE
                     IHPRFQTPGFSILVQGIWSMLLTLTGSYETLLSYVLFAMWLFHAMAVFGVIILRRKDG
                     NRPRPYRMWGYPVAPLLFTAFALAFVINTFISRPIPSLLGALIIASGIPTYWLWRRQA
                     RRLSRAGMA"
     gene            938..1945
                     /gene="hrcA"
                     /locus_tag="VFQ79_16045"
     CDS             938..1945
                     /gene="hrcA"
                     /locus_tag="VFQ79_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU0141234.1"
                     /translation="MNILHAIVQSYIETGEPVASRTISRRWSGGALSPASIRNVMADL
                     CDEGYLAQPHTSAGRIPTAKAYESYVRSIAEQRVLRAELQRIRTELGRLDTMEDRVEL
                     SSHILTEMTRNVGIAAAIPTSGQTLDQVELLTLGEQRVLMIVVTRDRMIRNRVVALEE
                     AVSQDELNSIRNYINRNFSGWILADVKKELKSRLEAESAAYDEILKKLILLYDKGLLN
                     LSFNPEVHLEGTSNLVGVGLQWTRETMRALFHALEEKKRILELLEQFMEKPEGEVAVQ
                     VGLGGAHPDLGEFSLIGLNVRLPSGLSAKIAVLGPMRMDYGNVISAVQHVGRAFQSLP
                     V"
     gene            2024..2575
                     /gene="grpE"
                     /locus_tag="VFQ79_16050"
     CDS             2024..2575
                     /gene="grpE"
                     /locus_tag="VFQ79_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU0141235.1"
                     /translation="MAIENKPIVDDPVDSGPSALPEGETEPAEETLLALVSERDRLAG
                     EKAELQDQLLRLRAEFDNFRKREERERMEFVEFAAMEAIKPLLPVLDDFERALKAESQ
                     DSEYAKGIELIYQGLLDTLKKLGVGPIVAEGQPFDPNLHHAVEMIKTDDFEDHTVVSE
                     YQRGYNFKGRLLRPAMVRVAVRP"
     gene            2579..>3164
                     /locus_tag="VFQ79_16055"
     CDS             2579..>3164
                     /locus_tag="VFQ79_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU0141236.1"
                     /translation="MNVSKRDYYEVLGVSREADEQSLKSAYRKLALKYHPDRNPGDKS
                     AEEKFKEAAEAYSVLTDPQKRSAYDRFGHQGLQGGGNGFNPDIFADFTDILGDFFGFG
                     FGDAFGGNSTRRRSRAQQGEDLRYDLEIGFEDAIKGMTADIQAPRLETCKSCSGSGAE
                     AHDGFTTCSRCRGRGEVIFQQGFLSVRQTCSQCGG"
BASE COUNT          665 a          871 c          959 g          669 t
ORIGIN      
        1 ctgctgaagg tggccgggtt ggcgctactc atttcgagtg cctttctgta tcgcggaccg
       61 gtggcgctcg aatgggcatc tccggcgtcg gtgctgtcgt tgcggagcgt agcgatcgcc
      121 atgctgggct gcttcctcgc atatgacggc tggcaataca ttgcattcgt ggccggtgag
      181 gtgcgaaatc cccaacggaa ccttccgctc gcgctggggc ttggaacggg tgccgtcatc
      241 atcctctacc tgctcgccaa cctggcgtat atgcgagtac ttccgctgag ccagatcgct
      301 tccaccgaac gcgtcgccgc cgtggtttcc gaacaaacaa tgggaagcgc cggcgcggct
      361 ctcgtcgccc tcacgatcat gctgtcctgc gcgggcgcgg ccaatggggc cattctttcc
      421 agcccgcgaa tttacttcgc ccaggcacgc gacggactgt ttttccagag attcgctgaa
      481 atccatcccc ggttccaaac accggggttt tccattctgg tgcagggaat ctggtcgatg
      541 ctcctgacgc taaccgggtc gtatgaaacg ctgctatcgt acgttctttt cgcgatgtgg
      601 ctctttcatg cgatggcggt gttcggcgta ataattcttc gccgtaagga tggcaatcgc
      661 ccgcgtccct accggatgtg gggctatccg gtagctccac tgctctttac agcgtttgcc
      721 ctggcctttg taatcaatac attcatctcc cgcccgatcc cgtcgctact gggggcactg
      781 attattgcaa gcggcattcc aacctactgg ttgtggaggc ggcaggcacg gcgcctatca
      841 cgggcaggca tggcttgacg ggttgatgtg tctattttac aatatgttaa caggggtgtt
      901 ccctctcgct atggttagtc gaaaaccatc ccgttcgatg aatattctgc acgctatcgt
      961 gcagtcctac atcgagacgg gcgaacctgt cgcgtcccgg acgatttccc ggcgctggag
     1021 cggcggcgcg ctcagcccgg catcgatccg caacgtgatg gccgatttgt gcgatgaagg
     1081 ctaccttgcc cagccgcaca cctccgcggg ccggatccca acggcgaaag cctatgaaag
     1141 ctacgtgcgt tcgattgcgg agcagcgcgt gcttcgagcg gaactccagc ggatccgtac
     1201 ggagttgggc aggctggaca cgatggagga ccgcgtcgag ctttcctctc atattctgac
     1261 ggaaatgacc cggaacgtcg gaattgcggc tgccatccct acctccgggc aaacgctcga
     1321 ccaggtcgaa ttactcacct tgggcgagca gcgtgtgctg atgattgtgg tcacgcggga
     1381 tcgtatgatc cgcaaccgcg tggtggcgct cgaagaggcc gtgtcgcagg acgagttgaa
     1441 ctccatccgc aattacatca accgcaactt cagcggctgg atcctcgcgg atgtaaaaaa
     1501 ggaactcaaa tcgaggctgg aggccgagag cgcagcctac gacgagatcc tgaaaaagtt
     1561 gatcctgctc tacgataagg gcttgctgaa cctgagcttc aatccggaag ttcacctgga
     1621 aggcacttcg aacctggtcg gagtcggttt gcaatggacg cgtgagacga tgcgggcgct
     1681 gttccacgct ctggaagaga agaaaagaat tctcgaactg ctggagcaat tcatggagaa
     1741 accggagggc gaggtggcgg tgcaggttgg tcttggcggc gctcatcccg atctcgggga
     1801 gttctcgctc attggtctga atgtccggct tccgagcggc ttatccgcca agatcgcggt
     1861 cctgggaccg atgcgaatgg actacggcaa cgtgatctcg gcggttcagc acgtgggacg
     1921 ggcctttcag agtctgcctg tataataaga agtagcaccg gttaggcgaa acagaggagt
     1981 tctccgctcc aagaacgtta ttttcctgga atgaagctca gacatggcaa ttgagaataa
     2041 acccatagtg gacgatccag tcgatagcgg tccatccgcg ctacccgagg gcgagacgga
     2101 accggcagaa gaaaccctgt tggcgctggt ctcggaaaga gatcgcctgg ctggggagaa
     2161 ggcggaatta caggaccagt tgctgcggct acgagccgag ttcgacaatt tccgaaagcg
     2221 ggaagagcgc gaacgaatgg aattcgtcga gttcgctgcg atggaagcga tcaaaccgct
     2281 tctaccggta ctcgacgatt tcgagcgcgc gctgaaagcg gaatcgcaag attccgagta
     2341 cgcgaaggga attgaactga tttaccaggg attgttggat accctgaaga aactcggggt
     2401 agggccgatt gttgcagaag gacaaccatt cgaccccaat ttgcaccatg ccgtggaaat
     2461 gattaagacc gacgatttcg aggatcatac cgtcgtgtcc gagtaccagc ggggctacaa
     2521 tttcaaagga aggctgctgc ggcccgcaat ggtaagagtt gccgtcagac cttagctcat
     2581 gaacgtgtcc aagcgagatt actacgaggt tctcggtgtc agccgggaag ccgacgaaca
     2641 atcgctgaag agcgcctatc gcaagctggc attgaagtat catcccgacc gcaaccccgg
     2701 cgacaagagt gccgaggaga agttcaaaga ggctgcggaa gcctacagtg tgctgaccga
     2761 tccgcagaaa cgctcggcct acgaccgctt cggccaccag ggtctgcaag gcggcggtaa
     2821 tggtttcaac cccgacattt tcgcggattt tacggacatt ttgggggatt ttttcggatt
     2881 cggtttcggg gatgcttttg gaggaaactc gacccggcgg cgtagtcgcg cccagcaggg
     2941 agaggacctg cgttacgatc tcgagatcgg tttcgaggat gccatcaagg gcatgacggc
     3001 cgacattcag gcaccgcgcc tggagacgtg caaatcctgc agtggcagcg gcgccgaagc
     3061 acacgacggc ttcaccacct gctcccgttg cagaggacgc ggcgaggtca tcttccagca
     3121 gggctttctt tccgtgcgcc agacctgttc gcaatgcggg ggac
//
LOCUS       DASTMK010000369         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_560899, whole genome shotgun sequence.
ACCESSION   DASTMK010000369 DASTMK010000000
VERSION     DASTMK010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560899"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFQ79_16060"
     CDS             <1..298
                     /locus_tag="VFQ79_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141237.1"
                     /translation="HFENFCNEDGIKYTGEISLTTLTDFRAGRRVTVKDGEEVTEREI
                     TGSTSGKELETMRAFCAFAKKHGCIGETTQRKCGRRKRRTALHCHSRPRKWTPS"
     gene            283..894
                     /locus_tag="VFQ79_16065"
     CDS             283..894
                     /locus_tag="VFQ79_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0141238.1"
                     /translation="MDAILKACDQMEDGNPNRLERTRARARAFCLVMLYSGLRISDTV
                     KLRRKAVDLTTGKMLLRVMKTGRKLYVRLGRSAIDALSALPGRGEYFFWNGESKLSTA
                     VGNARKTISRVLAVAKVAGHPHRFRDTFSVRLLEKGEDLRTVQLLLGHTSIKTTEKHY
                     APFVDSFQRILDAATSKLDFVSEALYQNVPKRRISGISRRLAS"
     gene            1057..2304
                     /locus_tag="VFQ79_16070"
     CDS             1057..2304
                     /locus_tag="VFQ79_16070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141239.1"
                     /translation="MPIHRRHFLKSSTTGAALALAGLSPGQAQTRNDTIQVGIIGPGA
                     RGTELLKECIELGSAGNARMVAVCDIWSRALERAARRVRESYGTEPKTYRHHEELLAD
                     KEIDAVIIATPDHQHARMLIQAVQAGKDVYCEKPMANVLEEANEALAVVQRSDRVVQM
                     GTQRRSFPRYQTARDLMRKGRIGNLVKVDICGNQYTPFRWSFKPEEFAAVRESDVDWK
                     AFLMGKPDRPFDPRIYRSFRLFREFSGAIADQWMSHDIDMVHFLTDELYPLSAVALGG
                     VYEYRDFRENPDTLQVAFQYGRKRSPFLVAYSACLNNSTGFGTDYLGTKGTIEVADRF
                     RLSGGVGKQPDAVKEDSELSAATGVQHHMANWFSAIRQRKQDAVYAPVEAGYGQSIAC
                     IMAIQSYWSGRRTTFDAAKQQIV"
     gene            2331..>3674
                     /locus_tag="VFQ79_16075"
     CDS             2331..>3674
                     /locus_tag="VFQ79_16075"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0141240.1"
                     /translation="MTWRKRICRISYLLLLAGAGPAAWSQTPPGPGATGENVRHDANQ
                     RYELFQKYLQRRAAEVSRANLSTIHSLEQWKREKPRVSREMRYMLGLDPMPAKTPLEA
                     KVTGVIERPTHRIEKIVLQSMPGLYVTGNLYLPKNAGGPLPTVLYLCGHSPSPHGAKI
                     HYQHHGVWFARNGYAAFLIDTIEYGELAGVHHGLHDLGMWYWLSLGFTPAGPEVWNAI
                     RAIDYLQTRKEVDADRIAITGMSGGGAMTWYTAAVDDRVKVAAPVCATWTVEQQVALD
                     HVRGNCDCIFFHNTFQADLPLAGALIAPRPLKMINATRDVVFPPAGYLDVFRRLEPVY
                     GWHGASGKLAQYEHDAPHEDILPFRKAANEWINRWLKQDNTPFDEGAIEQEPGEVLRV
                     LDHYPPGTINDSIHRRFIPVHELRPWPQRTAWERRRSELIEQIKNRVLRLLPSTKV"
BASE COUNT          774 a         1093 c         1088 g          719 t
ORIGIN      
        1 gcatttcgag aacttctgca acgaagacgg catcaagtac acgggcgaaa tctcgctcac
       61 aacgcttaca gacttcaggg cgggccggcg ggtcaccgtc aaggatggcg aggaagtcac
      121 cgaacgggag atcaccggct ccacgagcgg caaggagctt gagaccatgc gggccttctg
      181 cgccttcgcc aagaagcacg gctgcatcgg agagactacg caaaggaagt gcgggcgccg
      241 aaagaggagg actgccctac actgccattc acgaccccgg aagtggacgc catcctaaag
      301 gcgtgcgacc agatggagga cggcaatccc aacaggctgg agcgtaccag ggcacgggcg
      361 cgggcgttct gtctggtgat gctctactca gggctgcgga tctccgatac tgtgaagctt
      421 cgccgcaaag ccgtggatct caccacgggc aagatgctgt tacgggtgat gaagactggc
      481 cgcaaactct acgttcgcct ggggcggtcc gcaattgacg ctttgagcgc tttgccgggc
      541 cgtggtgagt acttcttctg gaacggagaa tcgaagctct ccacagccgt aggaaacgcg
      601 cggaagacta tttcccgcgt gctggccgtc gcgaaagtgg ccggacatcc gcatcggttc
      661 cgcgacacgt tcagcgtgcg gctgctcgag aagggtgaag accttcgcac agttcagttg
      721 ctcctgggac acacgagtat caagaccacg gagaaacatt atgcgccgtt cgtcgattcc
      781 ttccagcgaa ttctggacgc cgcaacatcg aagcttgatt ttgtttcaga agcactgtac
      841 caaaacgtac caaaacggcg catttctggc atttctcgcc gtttggcctc ttaacagaat
      901 caataagtta cttgttttca atgcggatat tgggttcgac tcccactagc ttccgccaaa
      961 caactaattt tctccgctcc tgccggcggc tatgaacatg ggacatcggc tccaatggta
     1021 gaattgccca catccggaag gttttctgaa atcaacatgc cgattcatcg tcgccatttt
     1081 ctgaagagtt cgactacggg ggccgctttg gccctggcgg gtctttcgcc gggccaggcg
     1141 caaacccgaa acgacaccat ccaggtgggt attatcggac cgggcgctcg cgggaccgag
     1201 cttcttaaag agtgtattga gcttggcagc gccggcaatg cccggatggt tgcagtttgc
     1261 gacatctgga gccgggcgct ggaacgcgca gcgcgccgtg ttcgtgaatc ctacggtacg
     1321 gagccgaaga cctaccgtca tcacgaggag cttctggccg acaaagaaat cgatgcggtg
     1381 atcatagcca ctcccgatca tcagcatgcc aggatgctca tccaggccgt gcaggcgggc
     1441 aaggacgtct actgcgagaa gcccatggca aacgttctcg aggaggcgaa cgaggccttg
     1501 gccgtcgtgc agcgctccga tcgggtcgtt cagatgggca cccagcgccg cagttttccg
     1561 cgctaccaga ccgctcgcga cctgatgcgc aagggccgca tcggcaatct ggtgaaagtc
     1621 gacatatgcg gcaatcaata cactccgttc cggtggtcct ttaagccgga agaatttgcg
     1681 gccgtgcggg agagcgatgt cgattggaag gcctttctga tgggcaagcc cgaccggcct
     1741 ttcgacccgc gcatctaccg gtcgttccgg ctgttccggg agttttccgg cgccatcgcc
     1801 gatcagtgga tgagccacga tatcgatatg gtgcacttcc ttacggacga gctttatccg
     1861 ctcagcgccg tcgccctggg aggagtctac gaataccgcg actttcgcga aaatccggat
     1921 acccttcagg tcgcttttca atatggaaga aagcggtccc cgttcctggt ggcatacagt
     1981 gcctgcctta acaacagtac aggttttggc acggattatc tgggtacgaa agggacgatc
     2041 gaggttgcgg atcgctttcg cctttcgggc ggagtcggaa agcagccgga cgcggtcaaa
     2101 gaggattccg agctttccgc agcaacgggc gtgcagcatc atatggccaa ctggttttcc
     2161 gcgatccggc aacgtaaaca ggatgcggtc tatgcgccgg ttgaggcggg ctacggtcag
     2221 tccatcgcct gcatcatggc gatccagtcc tattggagcg gccgccgcac cactttcgat
     2281 gccgcaaaac agcagatcgt ttagcttgct ccatggcggt gagcgatgtc atgacctggc
     2341 ggaaacgcat atgccgcatc tcgtatcttc tccttctggc cggcgccgga ccagcggcct
     2401 ggagtcaaac cccgcctgga cccggcgcca cgggagaaaa tgtccgccat gacgcgaacc
     2461 agcgttacga gctgtttcaa aagtatctac agcgccgggc ggcggaagtt tctcgagcga
     2521 acctgagcac gatccattcg ctcgagcagt ggaagcggga gaagccgcga gtgtcccgcg
     2581 agatgcggta catgctgggg ctggacccta tgcccgccaa gaccccgctc gaagccaaag
     2641 ttaccggcgt catcgagcgg cccactcatc gcatcgagaa gatcgttctc caaagcatgc
     2701 cagggctcta cgtcaccggc aacctttacc ttccgaaaaa cgccggcggt ccgttgccca
     2761 cggtgctcta tctgtgcggc cactcgccga gtccccacgg cgcgaagatc cactaccagc
     2821 accacggcgt ttggttcgcg cgcaacggct acgccgcatt tctgattgac accatcgaat
     2881 atggcgaact ggcgggggtt catcatggcc tgcacgatct cggcatgtgg tactggttat
     2941 cgctcggctt cacgccggcg ggtccggaag tctggaatgc catccgcgcc atcgactacc
     3001 ttcagacgag aaaggaagtg gacgcggacc gcatcgcgat caccggcatg tccggcggcg
     3061 gcgccatgac ctggtacacc gccgcggtcg acgaccgggt gaaagttgcg gcgccggtgt
     3121 gtgcaacctg gacagtcgaa cagcaggtgg cgctcgacca tgtccgcgga aactgcgact
     3181 gtatcttctt ccacaacacc tttcaggcgg atctgcctct cgccggggcg ttgatcgctc
     3241 cgcgcccgct caaaatgatc aatgccacac gagatgtggt gttcccgccg gcgggttatt
     3301 tggacgtctt tcgccggctc gaacccgttt acggctggca tggcgcttcc ggcaaactgg
     3361 cgcagtacga acacgacgct ccccacgaag acatccttcc gttccgcaaa gcggcaaacg
     3421 agtggattaa tcgctggctg aaacaggaca acacgccttt cgatgaaggc gctatcgagc
     3481 aggaacccgg cgaagtgttg cgcgttctcg atcattatcc gcccggcaca atcaacgaca
     3541 gcatccacag gcggttcatc ccggtgcatg agttgcgccc ctggccccaa cgaactgcgt
     3601 gggaacggcg gcgttctgag ctgatagaac agatcaagaa ccgggtgttg cgcctgctgc
     3661 cgtccacaaa ggtc
//
LOCUS       DASTMK010000370        11713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_434263, whole genome shotgun sequence.
ACCESSION   DASTMK010000370 DASTMK010000000
VERSION     DASTMK010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11713
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434263"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="VFQ79_16080"
     CDS             <1..1974
                     /locus_tag="VFQ79_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEU0141241.1"
                     /translation="EFTARALILGLIMTVILGAANAYLGLRAGMTIAATYPAAVIGMA
                     ILRIWRGSILEENMARTVGSIGESVAAGAIFTIPAFVISGAWPSFDFEHAYWPSTVLM
                     MVGGVLGILFVTLLRRVMVEDPELPFPESVAASEIHKAGQHGSKAAGILFSAMGFGAL
                     IYSLGVVKVFSASNDFMIRIGELGSTLLRLGRTAESPAIGVGGVTMVSAPNVSPAYLG
                     VGYIIGPRLAALNFSGGVVAWGLLVPLFIYFLGPQLQITLPADATAEDWAGAATSVWL
                     NIVRPIAVGGMLVGAAYTLFRMRKSLGAGIRRSISDLKKASGQKQATNRTELDLSSKT
                     VFLGLGATLICMVLLYTYFSGTLAGGVVAALVMIVLGFFFAAVSGNLVGLIGSSNNPI
                     SGLTLSTLIIAALLMVSIGVSGMTGVAAVLGVAAVVCVSSAVAGEMLQDLKVGHILGG
                     TPRSMQIGDFIGVAAASLLLYFPLLLLHQGNINAGGTGFGDRALSAPQAGLMASLAQG
                     IVGGDMPWPLVVVGIMMGFALIMVQVRSPMLFAVGMYLPLETTFAIFIGGVIRWITDS
                     MRERRAFNDAQKARVENAGVLTASGLIAGEALMGLVVATFRFFEWPLPVIFEHPPLLI
                     ALAVLVLLGLVMVRVPLANAGRPDEPAPPTAIM"
     gene            complement(1981..2484)
                     /locus_tag="VFQ79_16085"
     CDS             complement(1981..2484)
                     /locus_tag="VFQ79_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141242.1"
                     /translation="MPRRVVTYTAIVGLRLEYARFRFDPSMSRGDLARVLAANPPQWK
                     TARWPPPLTGTDADLSILALSMLKANLDFTNRLSDGTARQMESAIQVRFPGITSEEAG
                     ASGNYKRFPIEAEIWEDGSTSWGYEIPEAGEFPPGFEADPTGLEIFLRNVVRADLQAR
                     LEALGSK"
     gene            complement(2499..4019)
                     /locus_tag="VFQ79_16090"
     CDS             complement(2499..4019)
                     /locus_tag="VFQ79_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEU0141243.1"
                     /translation="MFKAQTRFAVILLSVSGWAAADVRLPSLISDHMLIQRDAPARIW
                     GKADPGEQVTVTLASSTRSTTADALGRWEVFLPPAQAGGPFELTVKGKNTLQVNDVLV
                     SDVWIASGQSNMVWQVSRSDNAQQEIASARFPRIRLFKAALKTSDYPLDDVEGAWTTC
                     NPDTVGAFSAVGYFFARDLHQKYGIPVGVIQSAWGGTPAESWISPTSLAADPALISVY
                     ADWAQLISDYPEARARYEIRLKEWEEKNKTSGGTPAPGRPGPPVGPGHPWEPSSLYNA
                     MIAPLTPYAIKGAIWYQGENNAHKNRSYVYRRLFQAMIQDWRRSWGQGNFPFLFVQLA
                     NYARTPAASEWPELREAQAMALDLSQTGMAVTIDIGDPNDIHPTNKQDVGHRLALAAR
                     HIAYGEEIVYSGPLFREVTEGKSLRVWFSHVGGGLTAKGGPLRAFEVAGRDKKYVPAN
                     AAIEGKTVIVSSESVAEPMYVRYNWADSPDGNLYNAEGLPASPFRSEEWTGPAMYR"
     gene            complement(4084..5166)
                     /locus_tag="VFQ79_16095"
     CDS             complement(4084..5166)
                     /locus_tag="VFQ79_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141244.1"
                     /translation="MRIFAVAALVACLGPGSLLADFSYDQSSKVTGGALASMMKVAGA
                     FSKKAREPIRSTVLVKGNRMATVGGSSTQIIDLDSETITDINLEKKTYSVITFAQMTQ
                     ALQQMARKMEGETKADMNFKASVKDTGQSKNISGLDTRQVILTVEIEGTDKQSGQTGA
                     MTVTSDMWLAAAVPGYDEVKEFQKRMAQKLDWSPGSTMPMMQPGMAKGMAELWKESSK
                     LEGMPVLMVVTMGAKGQGGEMPEGSAQSAGQQQAPPPPSAGEAAEGAAAGAVGSRLGR
                     LGGLGGLGGFGRRKKQQQEEQAAAPPPAEPSAQSQRAGSGTSAGALLELTTELASFSP
                     AAVDGSRLEVPSGFKQVESEMLKHLK"
     gene            5286..5906
                     /locus_tag="VFQ79_16100"
     CDS             5286..5906
                     /locus_tag="VFQ79_16100"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0141245.1"
                     /translation="MRFLALFCVMAAGLCAAEWKQLFNGKDLTGWKMVGPGRFVVENG
                     ELKTEGGMGLLFYEREKFGDATLRVVFRTTEGHGNSGVFIRLPEPPKDPWYGVHNGYE
                     VQIESGGDEWHCTGAIYSISKVTARPQTSGSGWNTMDIVLKGPKTTVFVNGQKVNEYV
                     EGQEVPPRKAWFEPVRGPRPEIGYIGLQNHDAKSTVFFREVSIKKN"
     gene            6019..6801
                     /locus_tag="VFQ79_16105"
     CDS             6019..6801
                     /locus_tag="VFQ79_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141246.1"
                     /translation="MPVPPIPPPLEQFGQRPFSFYPPLLNVDHNEWRFQRATWSEILV
                     RNTASGAEVWIPRRYLGEISRVDEPVMILGLLKELELRAGSVWPAEKRVIEMPRAVND
                     VRPVAGGTEPPSPAPVVGLRFESSSESRISKLIGGVLLIGIIACVVVVSLFRGGPAGT
                     RVNYTTVLQSEIGLTAHDDYHAVIRKLGTPSDDHWRSDKGELQYRILNYPQQGLSIIL
                     MGSERGNATYIGAMDKAWKVVHSVELPDGRNTRPMLMALKRF"
     gene            6878..8944
                     /locus_tag="VFQ79_16110"
     CDS             6878..8944
                     /locus_tag="VFQ79_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEU0141247.1"
                     /translation="MKKFVGHLIWAGIAVAGALAFGAIALERGEPINSIWLVVAAACT
                     YLVGFRFYAKFIAAKVMLLDNARATPAERLRNGHDFEPTNKWILFGHHFAAIAGPGPL
                     VGPTLAAQFGYLPGTLWIIVGAVLGGAVQDFIILFASMRRDGKSLGEMAREEIGKVGG
                     ITALLTVLLIMVILLAVIALVVVNALRGSPWGTFTIAATMPIAVFMGLYLRYWRPGKV
                     LEVSVLGFVLVVASIFGGQAVAHSEALAPWFTFSGMALAVAVIIYGFLASALPVWLLL
                     APRDYLSTFVKLGVVMMLAVGIVFVRPDLELPAFTQFVDGTGPIFAGKIFPFCFITIA
                     CGAISGFHSLISSGTTPKMIAKEWHAWPVGYGSMLLEGFVAIMAMIAACVLQPGVYFA
                     VNAPAGIVGATPEVAVQTITSWGYPVTADQMASLARDVGEQTLMHRTGGAPSLALGMA
                     HIFARSGGGQAILGFWYHFAIMFEALFILTIIDAGTRVGRFMLQDLLGSVYAPLGRTS
                     WMPGVIGASAAIVLAWGYFLVQGVRDPLGGINSLWPLFGIANQLLAAIALCVATTILL
                     KMHGVKYIWITCVPLVWLVVVTYTAAWQKIFSPVPRIGFLAQAAQLESALASGEIAAA
                     RIAETQARIFNERLDAAVCGIFLVLVTTILLDSIRVWVGILRGTRDATIHEAPYVPST
                     LRAEEI"
     gene            8941..9117
                     /locus_tag="VFQ79_16115"
     CDS             8941..9117
                     /locus_tag="VFQ79_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141248.1"
                     /translation="MKAVRAVGRFLLCLMKEIGDENAYQRHLAAHGRRHSPEEWRRFS
                     DHHLRAKYQRGKCC"
     gene            9182..10597
                     /locus_tag="VFQ79_16120"
     CDS             9182..10597
                     /locus_tag="VFQ79_16120"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU0141249.1"
                     /translation="MFAPHHRKVKILFGLSDVLLTALAFEAAYQTRVWMQLERVFFLT
                     VPTKALLLGFCALVWVLLGFWFEVYSKLDSAHPRTILRDTFRQCAIGFVAVVVFQFML
                     RLDLSRPFLGLFAVYSWMLLCLFRLYAGQLVGAIRREFGAPHYVVLVGLGPKAQHLGE
                     LLEQSAKYGIRIRGFLNDTTETAPRYVRLEQEYPVYPLADLPQLLRYQVIDEVIFAVT
                     PQRLGELEEVLLMCDEEGVNTRVLLDFFPHVNSDMHLERIGPAPMLSFSAAPNDELRL
                     FLKRATDIALAAAALVLLFPVMLAIAGLIKLTSPGPVIFRQERCGLNGRRFVFYKFRS
                     MCDRADEMKFALRHLNRKTTAFKIPDDPRLTPLGRYLRRFSIDEWPQLWNVLKGDMSI
                     VGPRPAVPEEVWEYKRWQRRRLRMRPGLTCLWAVSGRDALDFETWMRLDMQYIDSWSL
                     SLDWKIILRTIPRVLTGKGAY"
     gene            10601..11215
                     /locus_tag="VFQ79_16125"
     CDS             10601..11215
                     /locus_tag="VFQ79_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0141250.1"
                     /translation="MHLVPSQDDVVTVFRETGALRQGHFEYPNGLHSNEYLQVALAMR
                     YFQYAKTLSVGLSRLLRANPEIRAMIPELSIVAPATGGLPVAYGVSEALRAAQVYWAE
                     RENENEPLRFRQYLKQMPGEKVLLVDDILRSGRRLTELKKLVESNGAEVVGLAVVIYQ
                     PNPKTPSFDPLPFYYLAKLDALYSIDASSCDLCKRGVPLEQVWI"
     gene            complement(11228..11455)
                     /locus_tag="VFQ79_16130"
     CDS             complement(11228..11455)
                     /locus_tag="VFQ79_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEU0141251.1"
                     /translation="MTIDRYLRMIAGAFVLASLALGYWVHPGWFLFTAFVGLNLFQSA
                     FSNWCPMMTLLQKLGIRSCAHDLKSSAATHR"
     gene            complement(11469..>11713)
                     /locus_tag="VFQ79_16135"
     CDS             complement(11469..>11713)
                     /locus_tag="VFQ79_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141252.1"
                     /translation="VIDAGAVRFRPMMLTASAVVVGATVILFDPIFQGLAISLMAGEI
                     ASLLLSRMTVPIVYYVVHRRQTAPAAPAAPTAVLTH"
BASE COUNT         2079 a         3415 c         3675 g         2544 t
ORIGIN      
        1 gagttcacgg cacgggccct gattctgggc cttattatga ccgtcatcct gggtgccgcg
       61 aacgcgtacc tcggtcttcg ggccggcatg accattgcgg ccacttaccc cgccgcggtg
      121 atcggcatgg cgatcctgcg catctggaga ggctcgattc tggaagaaaa catggcgcgg
      181 acggttggtt ccatcgggga atcagtcgct gccggcgcca ttttcaccat tccagccttc
      241 gtgatctccg gcgcctggcc gtctttcgat ttcgagcacg cctattggcc ctccacggtt
      301 ctcatgatgg tgggcggcgt tttgggcatt ctcttcgtca cgctgctgcg gcgcgtgatg
      361 gtggaagacc cggagttgcc gttcccggaa tccgtcgccg cttccgagat tcataaagcg
      421 ggccagcacg gcagcaaagc ggccgggatt ctgttctccg cgatgggctt cggagcgctg
      481 atctactcgc ttggggttgt caaggtattt tcggcatcga acgatttcat gatccggatc
      541 ggcgagttgg gcagcactct gctgcgtctc ggcagaaccg ccgagtcccc ggccattggg
      601 gtggggggcg ttacgatggt ttcggctcca aacgtgagtc ctgcttacct cggtgtcggc
      661 tatatcatcg gcccgcggct tgccgctctg aatttttcgg gtggtgtggt ggcctgggga
      721 ctgctggttc cgctgttcat ctattttctg gggccacagc tccagatcac tttgcccgcg
      781 gacgctaccg cggaagactg ggccggcgcc gcgacctcgg tgtggctcaa catcgtccgg
      841 ccgattgccg tgggtggaat gctggtcggc gcggcctaca cgctctttcg catgagaaag
      901 agcctgggtg cgggaatccg gcgttcgatt tcggatctca aaaaagccag tgggcagaag
      961 caggccacga atcgcacgga gctcgacctg agttcgaaaa ccgtgttcct gggcctcggc
     1021 gccacgttga tttgcatggt cctgctgtac acgtattttt cgggtacgct ggccggcggc
     1081 gtagtggccg cgctggtgat gatcgtcctc ggcttcttct ttgccgcggt ttcgggcaat
     1141 ctcgtcgggt tgatcgggtc gtcgaataac ccgatttccg ggctgacttt atcgacgctc
     1201 atcattgcgg cattgctgat ggtttctatc ggtgtctccg gcatgacggg tgtggcggcc
     1261 gtgctcggtg tggcggccgt ggtttgcgtt tcttccgcgg tggcgggtga gatgctgcag
     1321 gatctgaaag tcggccacat tctcggcggc accccgcgca gtatgcagat cggcgacttc
     1381 atcggcgtgg cggcggccag tcttctgctc tattttccgc tattgctact gcatcagggc
     1441 aacatcaatg cgggcggaac cggctttggc gaccgtgcgc tatcggctcc ccaggcgggc
     1501 ttgatggcat cgctggcgca gggcatcgtc ggcggcgata tgccgtggcc gctggtggta
     1561 gtgggcatca tgatgggttt cgcattgatc atggtgcagg tgcgcagccc catgcttttc
     1621 gcggtgggca tgtacctgcc gctcgaaacc acattcgcga tcttcatcgg cggcgtcatt
     1681 cgctggatca cggactccat gcgagagcgg agggccttca acgatgctca gaaagcccgg
     1741 gtggaaaatg cgggggtgct taccgcttcc gggctgatag ccggagaagc gctgatggga
     1801 ctcgtcgtgg cgactttccg gttctttgaa tggccgcttc ccgtcatctt cgagcatccg
     1861 ccgctgctga ttgctctggc ggtgcttgtg ctgctcgggt tggtgatggt gcgggtgccg
     1921 ctggcgaacg cgggccggcc cgacgaaccc gcgccgccca ccgcgattat gtgacggggc
     1981 tcacttcgag ccgagtgctt ccagacgcgc ctggagatcg gcgcggacca catttctgag
     2041 gaagatctca agtccggttg gatccgcttc gaagccagga gggaactcgc cggcctccgg
     2101 gatctcgtag ccccaactgg tgctgccgtc ttcccatatt tccgcttcta tcgggaaccg
     2161 cttgtagttt ccggatgctc cggcttcttc ggaagtgatg cccgggaatc gcacttgtat
     2221 ggcagactcc atctgccgcg cggtcccatc ggacaacctg ttcgtgaaat ccaggtttgc
     2281 cttcaacatc gatagcgcca gaattgagag gtcagcgtct gtacctgtca gcggcggggg
     2341 ccaacgagcg gttttccatt ggggtggatt ggcagccagc acacgggcga ggtcgccgcg
     2401 agacatcgat ggatcgaagc ggaatcgcgc atattccagt cgcagaccca caatcgctgt
     2461 gtaagtaacc acgcgtcgtg gcattgcgga tcctgaagct accggtacat cgccggtccg
     2521 gtccattctt cggagcggaa aggtgaagcg ggcaatcctt cggcgttgta taggttgccg
     2581 tccgggctgt ctgcccagtt gtagcgaacg tacatcggct ccgcgacact ttcgctcgac
     2641 acgataaccg ttttcccctc gatggctgca ttagccggta cgtacttttt gtcgcggccg
     2701 gctacttcga aagcgcgcag cgggccgcct ttcgccgtca atcctccgcc cacgtggctg
     2761 aaccacacgc gcaaggactt gccttctgtt acctctcgaa acagcgggcc tgaatagaca
     2821 atctcttcgc cgtaggcgat gtgccgcgcc gccagcgcca gccggtgtcc cacatcctgc
     2881 ttgtttgtcg gatgtatgtc gttcgggtcg ccgatatcaa tggtgacggc catgccggtc
     2941 tggctgaggt cgagcgccat ggcctgcgct tcccggagct ccggccattc actcgccgcg
     3001 ggcgtcctgg cgtagtttgc gagctgcacg aagaggaacg gaaaattacc ctggccccag
     3061 gagcggcgcc aatcctgaat cattgcctga aaaaggcgcc ggtaaacgta cgagcggttc
     3121 ttgtgggcat tgttctctcc ctggtaccag atagcccctt tgatcgcata gggtgtaagc
     3181 ggcgcgatca tggcgttgta cagcgacgag ggctcccagg ggtggccagg tccgaccgga
     3241 gggccgggcc tgccgggcgc cggagtgccg cccgacgtct tgtttttctc ctcccattcc
     3301 ttcaaccgta tttcataccg ggcgcgagct tcggggtaat cggagatcag ttgggcccaa
     3361 tccgcatata cggaaatcag cgcggggtcg gcggcaagcg aggtgggact gatccaggat
     3421 tccgcgggag ttcctcccca tgccgattga atgacgccca ccggtatgcc atacttctgg
     3481 tgcaaatcgc gcgcaaagaa ataacccacc gcggagaatg cgccgacggt atcgggatta
     3541 caggtcgtcc aggcaccctc gacatcgtcc agcggatagt ccgacgtttt cagagcggct
     3601 ttaaaaagac ggatgcgcgg gaacctggcc gatgcaatct cctgctgcgc gttatcggaa
     3661 cggctcacct gccacaccat gttcgactgt ccggaagcga tccatacgtc gctaaccaga
     3721 acgtcattca cctgaagcgt atttttccct ttcacggtga gttcgaaagg gccgccagcc
     3781 tgcgccggcg gaaggaacac ctcccatcgg ccgagcgcgt ccgctgttgt gctgcgagtg
     3841 ctgctggcga gcgtcaccgt gacctgctcg cccggatcgg ctttccccca gatgcgcgcc
     3901 ggcgcgtccc gctgaatcag catgtgatcg gagatcagcg acggcaggcg tacgtcagcg
     3961 gcagcccatc ccgagacgga aagcagaatc actgcgaagc gagtttgtgc cttgaacaag
     4021 actcttcctc ctaattgccg ctccctgccg cgagcgcgag cgagcggtcg aagatccaag
     4081 aagctatttg aggtgcttga gcatctcgct ctcgacctgc ttaaagccgg acgggacttc
     4141 cagacgcgaa ccatccaccg cggcaggaga aaaactggcc aactcggtgg tcagctcaag
     4201 cagggctccg gcactcgtac cggaaccggc gcgctgggat tgggcggagg gttccgctgg
     4261 aggcggcgcc gcagcctgct cttcctgctg ttgcttcttc cggcgtccga aacctcccaa
     4321 tccgccgagt cctcccaagc gtcccaatct ggagccgacg gcgccggctg ccgcgccttc
     4381 cgccgcttca ccggccgagg gtggcggagg agcctgctgt tgcccggcgg attgcgccga
     4441 accttccggc atctcgccgc cctggccttt tgctcccatc gtcacgacca tcaggaccgg
     4501 catgccttcg agcttcgacg attccttcca aagctccgcc atacctttcg ccattccggg
     4561 ctgcatcatc ggcatcgtag agcctggtga ccagtccagt ttttgggcca tgcgcttttg
     4621 aaactctttc acctcgtcgt aacccggcac cgcagcggcc agccacatat ctgatgtcac
     4681 ggtcatggcg cccgtctgcc ccgactgctt gtcggttcct tctatctcga cggtcagaat
     4741 aacctgccgg gtatcaagac ccgagatgtt cttactttgg ccggtgtcct tcaccgaggc
     4801 cttgaaattc atatccgctt tggtttcgcc ttccattttg cgcgccatct gctgtagcgc
     4861 ctgggtcatt tgggcgaaag tgatcacgga ataggtcttc ttttccagat tgatatccgt
     4921 aatggtttcg ctgtccagat cgataatctg ggtactgctt ccgccgacgg tcgccatccg
     4981 gtttcccttc accagcacag tggaccggat gggctcccgc gcctttttgg aaaaagctcc
     5041 ggcaaccttc atcatgcttg ccagagcacc gcccgtaacc ttggatgact gatcgtagct
     5101 gaagtcggcc agaagtgagc cgggaccgag acacgcaacc aaagccgcaa ccgcaaatat
     5161 cctcatgcaa cctccaaaag gattatcgta cgcgcgtggc cggaagtgta gagtgcaggt
     5221 tacaggcgcg ggtactaccc gcccacgcgc tagaatgaaa atttcgacga acgggagaag
     5281 ctccaatgcg atttctcgca cttttttgtg taatggccgc cggtttatgc gccgccgaat
     5341 ggaagcagtt attcaatggc aaggatttga cgggttggaa aatggtgggc ccggggcggt
     5401 tcgttgtcga gaacggagag ctcaagacgg agggcggcat gggcctcctg ttttatgagc
     5461 gtgagaagtt cggggacgct acgctccggg tggtattccg aactaccgaa ggacacggga
     5521 attccggtgt gtttatccgg ctgcctgagc cgccgaagga cccctggtac ggggttcaca
     5581 acggctacga ggttcagatc gaatccggtg gagacgagtg gcactgcacc ggagccatct
     5641 actccatcag caaagtcacg gcccgccccc agacgtccgg ttccgggtgg aacactatgg
     5701 atatcgtcct gaaaggaccg aaaacaacgg ttttcgtgaa cggccagaaa gtcaacgaat
     5761 atgtggaagg acaagaagtg ccgccgcgga aggcatggtt tgaaccggtt cgcgggcctc
     5821 gcccggagat cggctacatc ggccttcaaa accacgacgc caaatccacc gtcttcttcc
     5881 gcgaagtcag cataaagaag aactaacgag cttgttgaaa aacatagaaa accgcttgtt
     5941 cgcgcgcgcg gctccgtaac gcctttgaca ccagacacgt gctgccgcgg gccggaagcg
     6001 gctaccatag aagcagccat gccggtgccg cccatccctc cgcccttgga gcaattcggc
     6061 cagcgtccgt tctcgtttta tcctccgctg ctgaacgtgg accataacga gtggcggttt
     6121 cagcgtgcca cgtggtccga gatcctggta aggaacaccg cgtcgggcgc cgaagtctgg
     6181 attccgcggc gttatcttgg cgagatttcc agggtcgatg agccggtcat gatcctgggg
     6241 ctgttgaaag agttggagct ccgggcgggg tcggtgtggc cggccgagaa acgcgtcatt
     6301 gagatgccgc gcgcggtcaa tgacgtccgt cccgttgcgg gaggtacgga acccccgtcg
     6361 cctgcgccgg tcgttggcct tcggttcgaa agcagttcgg aatcgaggat cagcaagttg
     6421 attggcggag tgctgctcat cggcatcatc gcgtgcgtgg tggtagtgag tcttttccgg
     6481 ggaggtcccg ccggcacgcg tgtcaactac acgacggtat tgcagtccga gatcggcctt
     6541 acggcgcatg acgactacca tgcggtgatc cgaaagctcg ggacgccgtc ggacgaccac
     6601 tggcgctcgg ataagggcga gttgcagtac cgcatcctca actacccgca gcagggactc
     6661 tccatcattc tgatgggcag cgaacgcggc aatgccacgt atatcggcgc catggataaa
     6721 gcctggaaag tagttcactc cgtggaattg cctgacgggc ggaatacaag gcccatgttg
     6781 atggccctca agcgattctg aagcaccgtc attctccctc ccgccgcgac agcgagagcg
     6841 gacgctctcc tccgcgcttc gatagaattc attccttatg aaaaaattcg ttgggcatct
     6901 gatctgggcc ggcattgccg tcgccggcgc gctggcgttc ggcgccattg cgctcgagcg
     6961 cggggagccg atcaacagca tctggctggt ggtcgcggcc gcctgcactt acctggtggg
     7021 gttccggttt tacgccaagt ttattgcggc caaggtgatg ctgctcgata acgcacgcgc
     7081 cacgcccgcc gaacgccttc gaaacggcca cgacttcgag ccgacgaaca agtggatcct
     7141 gttcggccat cattttgccg ccattgcggg ccctggtccg ctggtggggc ccacgctggc
     7201 ggcgcagttc ggatacctgc cgggcacgct ctggatcatt gtgggtgcgg tgctgggggg
     7261 agcggttcag gacttcatca ttctgttcgc atccatgcgg cgcgacggca aatccctggg
     7321 cgagatggcc cgcgaggaga tcgggaaagt ggggggcatc acggcgctgc ttaccgtgct
     7381 cctcatcatg gtcattcttc tggctgtgat cgcactggta gtggtgaacg cgctgagagg
     7441 gagtccgtgg ggcacgttca ccattgccgc caccatgccg atcgcggtgt ttatgggttt
     7501 gtacctgcgc tattggcgtc cgggaaaggt cctggaagtt tcggtattgg gcttcgttct
     7561 ggttgtcgcc agcattttcg ggggacaggc ggtggcccat tcggaagccc tggcgccctg
     7621 gttcacgttt tcggggatgg ctctcgcggt cgctgtgatc atctatggat tcctggcgag
     7681 tgcgctgccg gtgtggctgt tgctcgcccc gcgcgattat ctcagcacgt tcgtgaagct
     7741 gggtgtggtg atgatgcttg cggtaggaat cgtgttcgtc cggccggacc tggagctacc
     7801 cgcgttcacg caattcgtgg acggcacggg gccaattttc gcgggcaaaa tattcccgtt
     7861 ctgctttatc accatcgcct gcggcgccat ctcgggattt cactcgctga tctcgtcggg
     7921 caccaccccg aagatgatcg caaaggaatg gcatgcctgg ccggtcggct acggctcgat
     7981 gctgctcgaa gggtttgtag cgattatggc catgattgcc gcctgtgtgt tgcagccggg
     8041 agtgtacttc gccgtgaatg caccggcggg catcgtgggc gcgactccgg aagtggcggt
     8101 ccaaaccatc acctcctggg gctacccggt cacggcggat cagatggcat ctctcgcccg
     8161 cgatgtgggc gagcagacgc tgatgcatcg tacgggcggg gcgccgtcgc ttgctctcgg
     8221 catggcgcat atcttcgctc gcagcggggg aggacaggcg atcctgggct tctggtatca
     8281 cttcgccatc atgtttgagg cgctattcat tctcacgatt atcgatgcgg gaacgcgcgt
     8341 cgggaggttc atgctgcaag atcttctcgg cagcgtatac gcgccgctgg gccggaccag
     8401 ttggatgccc ggcgtcattg gcgcgagcgc ggccatcgtc ctcgcgtggg gttatttcct
     8461 ggtccagggg gtgcgggatc cgctgggggg tatcaactcg ctgtggccgc tgttcgggat
     8521 cgccaatcag ttgctggccg cgatcgcgct ctgcgtcgcg acaacgattc tgttaaagat
     8581 gcacggcgtg aagtacatct ggatcacgtg tgtgccgctg gtctggctgg tagtggtgac
     8641 atacacggcg gcatggcaga agatcttttc gccggtgccg aggatcggct tcctcgcgca
     8701 ggcggcacaa ctggaaagcg cgctcgccag cggagagata gcggcggccc ggatcgccga
     8761 aacacaggcg cgaattttca acgagcggct ggacgcggcg gtttgcggga tttttctggt
     8821 gctggtgaca acgattcttc tggattcgat ccgggtctgg gtgggtattc tgcgcgggac
     8881 ccgggacgcc accattcacg aggcgcctta cgtaccgtcg acgttgaggg cggaggagat
     8941 atgaaagccg tccgcgctgt cggccgcttt ctgttgtgcc tgatgaaaga gatcggcgac
     9001 gaaaatgctt atcagcggca cttggccgcg cacgggcggc gccattctcc ggaggagtgg
     9061 cggcggtttt cagaccacca ccttcgcgcg aaataccagc ggggcaagtg ctgttaaccg
     9121 tttcgagacg tgcctcttgc aggcgcacgg ccgctcgtgg ttctgatatt ctgatggacg
     9181 gatgttcgcg ccgcaccatc gcaaagtcaa gattctattc ggactttccg atgtcttgct
     9241 gacggccctt gcctttgaag cggcctacca gacgcgcgtg tggatgcaac tggagagggt
     9301 cttcttcctg accgtcccca cgaaggcgct tctgttaggt ttctgcgcgc tggtttgggt
     9361 gttgctgggc ttctggttcg aggtgtacag caagctcgat tcagcgcacc cgcggacgat
     9421 cctgcgggac acgttccgcc agtgtgccat cggctttgtt gccgttgtcg tttttcagtt
     9481 catgctgcgg ctggatttga gccgtccgtt cctgggtctg tttgcggtgt atagctggat
     9541 gctgctgtgc ctgttccggc tgtatgcggg gcagcttgtc ggggccatcc gtcgggaatt
     9601 cggcgctcct cattatgtgg ttctggtagg gcttggcccg aaggcgcagc atctcggcga
     9661 actgctggag caatccgcga aatacggcat ccggattcgc ggttttctca acgacaccac
     9721 cgagacggcg ccccgttatg tgaggctgga gcaggaatat ccggtctatc cgctggcgga
     9781 tctcccacag cttctgcgat accaggtgat cgacgaagtg atcttcgccg tgacgccgca
     9841 gcggctgggg gaactcgaag aagtgctgtt gatgtgcgat gaggaagggg tgaacacgcg
     9901 cgttctcctc gacttctttc cgcatgtgaa cagcgacatg cacctggagc ggatcggtcc
     9961 cgctcccatg ctgagctttt ccgctgctcc caatgatgaa ctgcgcctgt tcctgaagcg
    10021 cgcgaccgac atcgcgctgg cggcggcggc tttggtgctt ctgttcccgg tcatgctggc
    10081 cattgcgggt ttaatcaaac tcacgtcgcc gggtccggtg attttccgcc aggagcggtg
    10141 cgggttgaac ggacggcgtt ttgtctttta caagttccgg tccatgtgcg accgtgccga
    10201 cgaaatgaaa ttcgcgctgc gccacctgaa ccgcaagacc acggccttca aaattcccga
    10261 cgacccgcgg ctgacgccgc taggccgtta cctgcgccgt ttctcgatcg acgaatggcc
    10321 gcaattgtgg aacgtattga aaggcgacat gtccatcgta ggcccccgcc cggcggttcc
    10381 ggaagaggtg tgggaataca agcgctggca gcgcaggcgc ctgcgcatgc ggcccgggct
    10441 cacctgtctg tgggccgtca gcggccgcga cgcgctggac ttcgaaacct ggatgagact
    10501 ggatatgcag tatatcgaca gttggtcgct gtcgctcgat tggaaaatca ttcttcgtac
    10561 gattccccgg gtgttgaccg ggaagggagc ttattaagct atgcatctcg tcccctcgca
    10621 ggacgatgtt gttacggtgt tccgggagac cggcgcgctg cgccagggcc atttcgaata
    10681 cccgaacgga cttcattcca acgaatatct tcaagtagcg ctcgccatgc gctattttca
    10741 gtacgccaag acgctcagcg tgggtcttag caggctgctc cgggcgaacc cggagatccg
    10801 ggcgatgatt ccggagcttt cgattgtggc gccggccacc ggagggctgc ccgtggcgta
    10861 cggcgtttcg gaggctttgc gagcggcaca ggtatactgg gctgagcgcg aaaacgaaaa
    10921 tgagccgttg cgtttccgcc agtatctgaa acagatgccc ggagaaaaag tgctgctggt
    10981 ggatgacatt ctccggtcgg gccgcagact gacggaactg aagaagctgg tcgaatcgaa
    11041 tggcgcggag gtcgtcgggt tggcggtggt catctatcag ccgaacccga agacacccag
    11101 cttcgatcct ctgccgtttt actatctggc caaactcgat gcgctttatt cgatcgacgc
    11161 ctcttcctgc gatttgtgca agcgcggagt tcctctggaa caggtctgga tctaagccgg
    11221 agaccgctca ccggtgagtg gcggcgctac tctttaagtc atgcgcgcag gatcggatgc
    11281 ccagcttctg gagtagcgtc atcatcggac accagttgct gaacgccgat tggaacaggt
    11341 tcaagccgac aaaagcggtg aacaggaacc agccggggtg aacccaatag ccgagggcca
    11401 ggctcgccag gacgaaggcg ccggcaatca ttcgcaagta acgatcgatc gtcacaatca
    11461 aatcctcctt aatgtgtgag aacagcggtt ggcgcggccg gcgcggcagg tgccgtctgc
    11521 ctgcggtgga ccacgtaata aacaatgggg acagtcatac gcgacagcag cagagaagcg
    11581 atttcgcccg ccatgagaga gatggccagc ccctgaaaaa tcgggtcgaa caggatgacc
    11641 gtggcgccca cgaccaccgc gctcgccgta agcatcatcg gacggaaccg gacggcgccg
    11701 gcatcgatca ccg
//
LOCUS       DASTMK010000371         8641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_274641, whole genome shotgun sequence.
ACCESSION   DASTMK010000371 DASTMK010000000
VERSION     DASTMK010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274641"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFQ79_16140"
     CDS             complement(<1..581)
                     /locus_tag="VFQ79_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0141253.1"
                     /translation="MYRICRFLLLLSAAVPLLAQADGKLRIIAFGAHPDDCDSRAGGT
                     AAKYAKLGHHVKFVSVTNGDAGHQSEGGGALAKKRREEAREAGRRIGIEYETLDNHDG
                     ELLPTLAVRHQVIRKIRQWNADVVMAPRPNDYHPDHRYTGVLVQDAAYMVVVPNVCPD
                     TPPLRKNPLFLYFQDGFQRPNPFRPDVAVAIDDV"
     gene            complement(648..1586)
                     /locus_tag="VFQ79_16145"
     CDS             complement(648..1586)
                     /locus_tag="VFQ79_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141254.1"
                     /translation="MDRRDLIKGAGTLAALLAAADAAAGTDAPADRVPDAQGGPVLPS
                     VLFGRHRITRLIAGANPIYGHSHFNYLFNAHMVEYHNTPRVISFLKQLEKAGLTTWQA
                     SWSERLEKDWLEYKQQGGTLQLLLLSRPTFNQQSEMLKRAMKLNPIGIAQHGGSTKQL
                     WESGKFDLAKDYLSRIRDTGAMAAISCHEPKVIEYIEEKGWDLDYYMTALYNIVRPQS
                     EFAKILGEVPYGEVYLASDPPKMMSVIRKTKRPCLAYKVLAAGRTVSSPAQVKSRIAA
                     ALDGIKPGDALIIGMYQRFNDQIGQTAQFVRELLKA"
     gene            1718..2488
                     /locus_tag="VFQ79_16150"
     CDS             1718..2488
                     /locus_tag="VFQ79_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0141255.1"
                     /translation="MHVLERFKLDGKTALVTGCRRGIGKAIAVALAEAGADIAGVSAS
                     LETAGSAVEREVEKAGRRFRGYACDFANRDAVYKFIERVKSEVPPIDILVNNAGTILR
                     SPAAEHPDEDWDRVIEINLNAQFILSREFGGDMVARGSGKIIFIASVLSFQGGIYVPG
                     YAASKGAIASLMKALANEWAGRGVQVNGIAPGYIRTDNNEALRTDAARNAAILARIPA
                     GRWGEAEDLQGAAVYLASSASNYANGTILLVDGGWMGR"
     gene            complement(2504..5278)
                     /locus_tag="VFQ79_16155"
     CDS             complement(2504..5278)
                     /locus_tag="VFQ79_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEU0141256.1"
                     /translation="MANYHSIEGTPRGRNTALIVLLALVVLTFVLGRSILSYLIEYRW
                     WHELGQTSTWFTMLGYSIAPLVLATLIAAIVLWTAHSMALRFAGTSLRYYPLYAKLSI
                     LGALVLGFFFAAVVTDTWTVVRFFGGSRLDVQENAWHDPVFGHSLVFYFFQVPFYSLL
                     LRFVLGLSVVAALVYWLTARGWQLREQMPRWQDKGVIELQDLGLSGAIESRFLRGLAV
                     VFLIGLAVKFYLDRYTMLLNEHAFMVGVDWVNEYVSLPLQWLTILGAVAAAALVWMRR
                     PKLAAAMLLVLPVRALVPVIANAVYVRPNEISIQRPYIERHIQATRSAFRLDRNAKEI
                     DFPAKLDTKVDRTKHQALLENVRLWDWRAFHDTVTQIQALRPYYAFADSDVDRYQISG
                     KINQVLLSPREFDIRQLAGAQASWVNQRFIYTHGYGLVMAEANRITPEGLPVLFVRDA
                     PPVVETDSLRLTRPEIYYGEVVHEPVFVRTAQPEFNYPSGSENVHSRYEGKGGFPVSS
                     WPMRIAAAVTFGDWNIVLTQYLTPESRMMIRRNISGRLRSLAGFINWDPDPYLVLTDE
                     GRLVWMIDGFTTSRAHPYSRSIQMQGVGEFNYMRNSVKATIDAYDGSIDLFIFDSADP
                     IIQSYAKLFPALFKPAAEMPADMRRHARYPEVIFRAQAEIYRTFHMRDPESFYNKEDL
                     WDLGKNISGQGGRAEAVNPTYVVATMPGSNVPEFLLVIPFTPRNKDNLIGLMMARCDG
                     ENLGELVFLQLSKQELIFGPMQIEARINQDQFIAKDLALWSQQGSQVLRGHILVLPLE
                     DSFLYVEPIYIQASEARMPQLRKIVMAMGNRLIYADNYEQAVAALTGTEAGEVGTIPV
                     SSEIPPPSAMAPSASAPAAQPATAPPPTDKRIETIRDHLRRYRDFAAQGRWAEAGKEL
                     EAIQSVAGSR"
     gene            complement(5287..6576)
                     /locus_tag="VFQ79_16160"
     CDS             complement(5287..6576)
                     /locus_tag="VFQ79_16160"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU0141257.1"
                     /translation="MARLLIKNGRVMDPASSLDRVADVLIEGDKIVGIDENTDAAGAE
                     VIDAAGLIVAPGFIDMHVHLREPGFEHAETIETGSRAAAAGGFTSVCAMPNTAPINDD
                     ATVTRYIVERARRYAVVNVFPIGAITKGSAGKELAAIGSMKEAGIVAISDDGRPVMNA
                     RVMRRAMEFAREFSLPVIDHCEDLNLSAGGDMHEGFQSVRLGLGGIPACSEEVIVARD
                     ILLAELTGARFHVAHISARNSVRMVAAAKSRGLKVTCEATPHHIALSDSDMIPYDSNY
                     KMKPPLRSACDVSAVVEGIVSGTVDVIATDHAPHAGSEKMQEFEKCPFGITGLETAIG
                     VVLEHLVHTGKIGISKMIEMFTVKPASIVGLKGRGTLAPGSFADVTIIDPELTWTFDA
                     NQSCSKSRNTPFDGHTFRGGAVMTVVNGNCAWRRDIA"
     gene            complement(6579..7490)
                     /locus_tag="VFQ79_16165"
     CDS             complement(6579..7490)
                     /locus_tag="VFQ79_16165"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU0141258.1"
                     /translation="MSAGLLGIEDLERGEIEAILARAKEFQRLGSQARKRLDILSGRM
                     VVNLFYEASTRTRTSFEIAAKRLGADAVSITAAGSSVSKGESLVDTLNTLAAMRPDAI
                     VMRHAASGAPHFLARHLHMPIVNAGDGTHEHPTQALLDARTILDCRGSLEKMRIAIIG
                     DIAHSRVARSNIYLLSKFGADIVLCGPASLLPPEFASVAPGVTLTHDIREAIRDTDVV
                     MMLRVQLERQHEAAFPASEYFQFYGLTLEHLNVAKPDVIVMHPGPMNRGREIASEVAD
                     SQRSVILNQVENGVVVRMAVLERVLGN"
     gene            complement(7503..8054)
                     /gene="pyrR"
                     /locus_tag="VFQ79_16170"
     CDS             complement(7503..8054)
                     /gene="pyrR"
                     /locus_tag="VFQ79_16170"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU0141259.1"
                     /translation="MTPTREKTQLMSASEIDRTLVRLAHEILEKTQDLDKLAFIGIQR
                     RGVPLAYRLAKKIQSLENRDIPVGILDISLYRDDLSTIGAAPVVNGTDIAFPVTGKDV
                     ILTDDVLFTGRTVRAALDALFDHGRPSRVQLLTLIDRGHRELPIEAGYVGRKVQTTSD
                     EIIEVKFKEIDDSEKVLLVERIS"
     gene            complement(8133..>8641)
                     /locus_tag="VFQ79_16175"
     CDS             complement(8133..>8641)
                     /locus_tag="VFQ79_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEU0141260.1"
                     /translation="YGLISDVKMLNFFRFVGMTSAITLAVLVILSVFVQNFWCRYLCP
                     YGALMGLAAFFSPARIRRNPETCIDCAKCAKACPSLLPVDRLITVQSVECTGCLECVA
                     VCPAEDALHMSLPRKRVLPAWAMAAAVCAVFLGVVAYAQLAGYWHTPIADAIYFELIP
                     RAHEFGHP"
BASE COUNT         1775 a         2542 c         2596 g         1728 t
ORIGIN      
        1 acgtcatcga tggcgacagc cacatccggg cgaaacggat taggtctctg aaaaccgtcc
       61 tggaaataaa ggaagagcgg gttcttccgt aaaggcggtg tatcgggaca gacgttcggc
      121 accactacca tgtaagcggc atcctgtacc agcacgcccg tataacggtg gtcggggtga
      181 taatcgtttg gccgcggggc catgacaacg tcggcgttcc actggcggat tttccggatc
      241 acctggtggc ggacggcaag agtgggaagt aattctccgt catgattgtc gagcgtctcg
      301 tactcgattc caatccggcg gccggcttcg cgcgcctctt cgcgccgttt tttggccagg
      361 gccccgcctc cctcggactg atgcccggca tcgccgttcg taaccgacac gaacttgacg
      421 tgatggccga gctttgcgta tttggcggcg gttccgcccg cccggctatc acagtcgtcc
      481 gggtgcgcac caaaagctat gatgcgcagc tttccgtcgg cttgggccag cagcggcacg
      541 gcggcggaca aaagaaggag aaatcgacaa attctataca taaacagaaa tccctatcag
      601 aaaacatcat atacgcagtt gccgccggtt gcgcgaggaa ggtgagccta tgctttcagt
      661 aactcgcgca cgaactgcgc cgtctggccg atctgatcgt tgaaacgctg atacatgccg
      721 atgatcagag catcgcccgg tttgatgcca tcgagcgcgg cggcgatacg gctcttaacc
      781 tgagccggcg aacttacagt ccggcctgcg gccagcactt tgtaagccag gcatggtctt
      841 ttcgtttttc ttatgaccga catcattttg ggcggatcgg aagccagata aacctctcca
      901 tatggcacct cgcccagaat tttggcgaac tcggactgcg gccgcacgat gttatagagc
      961 gcggtcatgt aatagtcgag atcccagcct ttctcctcta tatactcaat tacttttggc
     1021 tcgtgacacg aaatggctgc catcgcgccc gtgtcgcgaa tgcggctcaa atagtccttg
     1081 gcgagatcga acttgccgga ttcccacaac tgtttggtgc tgccgccatg ttgcgcgatg
     1141 ccaatcggat tcagcttcat cgcgcgtttc agcatctccg actgctggtt gaacgtggga
     1201 cgggagagca gcagcagttg cagtgtgccg ccctgttgtt tgtattcgag ccagtccttc
     1261 tccagacgct cgctccagct tgcctgccac gtggtcagac cggctttctc caactgtttg
     1321 aggaaggaga tgacgcgcgg cgtgttgtga tactccacca tgtgcgcatt gaacaggtag
     1381 ttgaaatggg agtggccgta aatcgggttt gcgccggcaa tcaaccgcgt gattcgatgg
     1441 cggccgaaca ggaccgacgg cagtaccggc cctccttgtg cgtccggcac gcggtcggct
     1501 ggggcatcgg tccccgccgc ggcatcggcg gctgccaata acgcggccag agttcctgct
     1561 cctttgatca gatcgcggcg atccatgcta gggtcatcga tcggctcaag tattggcata
     1621 ctgctccttg tgaaggaaat gatagctcac tccgccggtg cgaatgcctg ttgttttgtc
     1681 accgccgcag ggtagagcct gatatcggag aacaactgtg cacgttctgg aacgatttaa
     1741 gctggacggc aaaaccgcgc tcgtcacggg ttgccgtcgt ggaataggaa aagccatcgc
     1801 ggtggcgctg gcggaagcag gcgctgacat cgccggtgtc agcgcatcgc ttgaaacggc
     1861 gggaagtgcc gtggagcgcg aagtagaaaa agcaggacgg cggtttcgcg gctatgcctg
     1921 cgacttcgca aatcgggacg ctgtttacaa gttcatcgag cgcgttaaaa gcgaggttcc
     1981 cccaatcgat attctcgtga ataacgccgg caccatcctg cgctcaccgg cggccgaaca
     2041 tcctgacgag gattgggacc gggtgatcga aatcaatctc aacgcgcaat tcattctgag
     2101 ccgggaattc ggaggggaca tggtggcgcg cggctcgggg aagatcattt ttatcgcatc
     2161 ggtattgagc tttcagggcg gtatttacgt gccgggttac gcggccagta aaggcgccat
     2221 cgcatcgctg atgaaagcgc tggcgaacga atgggcgggg cgcggcgtcc aggtcaatgg
     2281 cattgcgccg ggctacatac gaacggataa caacgaagcg cttcgtaccg atgcggcccg
     2341 taacgcggcc attctggcgc gaattccagc ggggcgttgg ggggaagcgg aggatttgca
     2401 gggcgccgcg gtttaccttg cgtcgtccgc ttccaactat gcgaatggaa ccatcctgct
     2461 tgttgacggc ggctggatgg gacgttaaac ggcggctccc ggcttaccgg ctcccggcaa
     2521 cggactgaat cgcttcgagc tctttccccg cttcggccca gcggccctgg gcggcgaagt
     2581 cgcggtagcg tcgcagatgg tcgcgaatgg tttcgatgcg tttatccgtt gggggcggcg
     2641 cggttgccgg ttgtgccgcc ggtgcggagg cagagggcgc catcgcggac ggcggtggga
     2701 tctcactgga aaccggaatc gtccccactt ctccggcctc cgttcccgtg agcgcggcga
     2761 ccgcctgctc gtaattgtcc gcatagatga gacggttgcc catagccatc acgattttcc
     2821 ggagctgcgg catgcgcgct tcggacgcct ggatgtagat cggctccaca tacaggaaac
     2881 tatcctccaa cggcaacacc agtatgtgcc cgcgcaacac ctgcgagccc tgctgactcc
     2941 acagcgccag gtccttggca atgaactggt cctggttgat tcgtgcctcg atctgcatcg
     3001 ggccgaaaat cagctcctgt ttggatagct gcaggaacac gagttccccc aggttttcac
     3061 cgtcgcaccg cgccatcatc aggccaatca ggttgtcctt gttgcggggc gtgaacggga
     3121 tgacgagcag aaactcgggc acgttggagc ccggcatggt cgccaccaca tatgtcgggt
     3181 tcacggcttc ggcgcgcccg ccttgaccgg atatgttctt gccgagatcc cacaagtctt
     3241 ccttgttata aaaggactcc ggatcccgca tgtggaaggt gcggtaaatc tccgcctgcg
     3301 cgcggaagat cacttccgga taccgcgcat gtcggcgcat gtcggcgggc atctccgcgg
     3361 ctggcttgaa gagggcgggg aacagcttcg cgtacgactg aataatgggg tcggcggaat
     3421 cgaagatgaa gagatcgatg cttccgtcgt aggcatctat cgtcgccttc acggaattcc
     3481 gcatgtagtt gaattcgccc acaccctgca tttgtatgct gcgcgaatag ggatgcgctc
     3541 tcgacgtggt gaacccgtca atcatccaaa ccaggcgccc ctcgtcggtg agcacaaggt
     3601 acggatcggg atcccagttg atgaaccccg ccagtgaccg taagcgcccg ctgatattcc
     3661 ggcggatcat catccggctt tccggcgtca gatattgcgt cagcacgatg ttccaatcgc
     3721 cgaacgtcac ggcggcggcg atgcgcatgg gccaggacga aaccggaaaa ccgccctttc
     3781 cttcgtagcg cgagtgcacg ttctccgagc cggaaggata gttgaactcg ggttgggccg
     3841 ttcgcacaaa taccggttca tgcacaacct caccgtaata gatctccggc cgggtcaggc
     3901 gcaatgagtc cgtttccacc acgggaggag cgtcgcggac gaacaggacc ggaagtcctt
     3961 cgggggtgat gcggttcgcc tcggccatca ccaggccgta gccgtgtgta tagataaagc
     4021 gctggttcac ccaactcgcc tgtgctcccg ccaactgcct gatgtcaaac tcacgcggcg
     4081 acagtagaac ctggttgatt ttgcccgata tctgatagcg gtccacgtcg gagtccgcga
     4141 aggcgtagta ggggcgcagt gcctgtatct gcgtgaccgt atcgtggaag gcccgccagt
     4201 cccagaggcg gacgttttcc agcaatgcct ggtgcttcgt ccggtccacc ttcgtgtcca
     4261 gctttgcggg aaaatcgatc tccttcgcgt tgcggtcgag gcggaacgcc gaacgggtag
     4321 cttgaatgtg ccgttcgatg tagggccgct gaatcgaaat ttcattgggc cggacataga
     4381 cggcattcgc gataaccggc accagtgcac ggaccgggag aaccagcagc atcgccgccg
     4441 cgagtttggg acggcgcatc cacacaagag ccgccgcggc gacagcgcca agaatggtca
     4501 gccattgaag cggaagactg acgtactcgt tcacccagtc gacacccacc atgaaggcat
     4561 gctcgttgag cagcatcgtg tagcgatcca ggtagaattt caccgccagt ccgataagaa
     4621 acaccaccgc cagtccccgc agaaaacggg attcaatggc tccgctcagt ccaagatcct
     4681 ggagctcgat aacgcccttg tcctgccagc gtggcatctg ttcgcgcaac tgccatccgc
     4741 gagccgtaag ccagtacacg agggcggcga ccaccgacag tcccagcaca aatcgcaaca
     4801 ggagggagta aaacgggacc tggaaaaaat agaaaacaag cgaatgtccg aaaacggggt
     4861 cgtgccaggc gttttcctgg acgtccaacc gcgagcctcc gaagaaacgc accactgtcc
     4921 aggtatcggt caccacggcg gcgaagaaga agcccagcac gagcgccccc aggatggaga
     4981 gcttcgcata aagggggtag tatcgcaggc ttgttccggc gaacctcagg gccatgctat
     5041 gtgcggtcca aagcacgatg gcggcgatga gtgtagccag cacgagcggt gcgatgctgt
     5101 agcccagcat ggtgaaccag gtggaagtct ggcccagttc gtgccaccac cggtattcaa
     5161 tcagataaga aagaatggac cggccgagta cgaaagtcag aactacgagc gcgagcagaa
     5221 cgattagagc cgtgttgcgg ccgcgcggag tgccttcaat cgagtggtag ttggccaagc
     5281 cccattctat gcaatatcgc ggcgccaggc gcaatttccg ttcaccaccg tcattaccgc
     5341 gccgccgcgg aacgtgtggc cgtcaaacgg agtattgcgg ctttttgaac aggactgatt
     5401 ggcgtcgaat gtccaggtga gctccggatc gatgatcgtt acatcggcga acgagccggg
     5461 cgcaagcgtc cccctgcctt tcagaccgac gatggaagcg ggcttcactg tgaacatttc
     5521 gatcatcttc gagatgccga tctttccggt gtggaccagg tgctccagca ccacgccaat
     5581 cgctgtttcg aggcctgtga tgccgaacgg gcacttctcg aattcctgca tcttttcgct
     5641 tcctgcatga ggggcgtggt cggtcgcgat cacgtccaca gtgcctgaca cgattccctc
     5701 taccaccgcc gacacatcgc aggccgatcg caacggcggc ttcattttat agttgctgtc
     5761 gtagggaatc atgtccgaat cgcttaacgc gatgtgatgg ggcgttgcct cgcaggtgac
     5821 tttcaatccc cgcgatttgg cggcggcgac catgcgcacg gagttccggg ctgaaatgtg
     5881 ggcgacgtga aagcgggctc cggtcagctc cgccagcaga atatcgcgcg cgacaatgac
     5941 ttcctccgaa cacgcgggaa tcccgcccag gccgaggcgc acggattgaa agccctcgtg
     6001 catatcgccg ccggcgctca ggttcagatc ttcgcaatga tcgatgacgg gcagcgaaaa
     6061 ctcgcgcgcg aactccatgg cgcgccgcat cacgcgggca ttcatcaccg gacgcccatc
     6121 gtcggaaatg gcgacgatac cggcctcttt cattgagccg atggcggcca gttctttgcc
     6181 cgcgctgcct ttggtaatag ctccgatggg gaacacgttc acgacggcat accggcgcgc
     6241 gcgctccaca atgtaccgcg tgacggttgc atcgtcgttg atcggggcgg tgttgggcat
     6301 cgcacagaca gacgtgaagc caccggcggc ggcggcgcga gaacctgtct cgatcgtctc
     6361 ggcatgctcg aaaccgggtt cacgcaggtg aacgtgcatg tcgatgaaac caggcgccac
     6421 gatcaacccc gccgcgtcga tgacttcggc tcccgccgca tcggtatttt catcgatccc
     6481 cacgatcttg tccccttcga tcagcacgtc ggcgactctg tcgagcgaag aagcgggatc
     6541 catcacgcgg ccgttcttta tcaaaagcct tgccataatc agttccccag cacccgctcg
     6601 agcaccgcca ttcgcacgac aaccccgttc tccacctggt tgagaatgac actccgctgt
     6661 gaatcggcga cttccgaagc gatctcgcgg ccgcggttca tcggcccggg gtgcatgacg
     6721 attacgtccg gctttgccac gttcagatgc tccagcgtca agccgtagaa ttgaaagtac
     6781 tcgctggcgg gaaaagcggc ttcgtgctgc cgttcaagtt gtacgcgaag catcatgacg
     6841 acatcggtgt cgcgaatcgc ttcgcgaata tcgtgcgtga gcgtaacacc cggtgcgaca
     6901 ctcgcgaatt ccgggggcag cagcgaagcc ggaccgcaaa gaacgatgtc ggcgccgaat
     6961 ttcgataaca gataaatgtt agagcgtgcg acgcggctat gggcgatgtc gccgatgatc
     7021 gcgatgcgca tcttttcaag cgaaccgcgg cagtcgagta tcgtgcgggc gtccagcagc
     7081 gcttgcgtgg ggtgctcatg cgttccgtcg ccggcgttga cgatcggcat gtgcaaatgc
     7141 ctggcgagaa agtgcggagc accggaggcc gcgtgccgca tgacgatggc atccggccgc
     7201 atcgccgcca gcgtgttcag cgtgtccacc aggctttcgc cctttgaaac gctggacccg
     7261 gcggcggtga tggatacggc atccgcaccc agtcttttgg cggcgatttc gaaactggtg
     7321 cgggtacggg tagaggcttc atagaagaga ttgaccacca tccgcccgct caggatatcc
     7381 aatcgtttgc gagcctgact tcccaggcgc tgaaactcct tcgcccgggc caggatcgct
     7441 tcgatctcgc cgcgctccag gtcttcaatc cccaacaatc cggcgctcat gcgtcctccc
     7501 ggtcaggaga ttctttcaac gagcagaacc ttttcgctat cgtcgatctc cttgaacttc
     7561 acttcgataa tttcgtccga tgtggtctga actttgcggc ccacgtaacc ggcctcaatg
     7621 ggcagttcgc ggtggccccg gtcgatcagc gtcagaagtt gaacccgtga gggcctgccg
     7681 tgatcaaaca gcgcgtcgag agcggcgcga acggtgcgtc ccgtgaacag cacatcgtcg
     7741 gtcaaaatga cgtccttgcc ggtcaccggg aaggcgatat ccgtgccgtt cacgaccgga
     7801 gcggccccga tcgtcgacag gtcgtcgcga tacaaactga tatcgaggat gccaaccggg
     7861 atgtcccggt tttcgagaga ctggattttt tttgccagcc ggtatgcaag cgggactccc
     7921 cggcgttgaa ttccgatgaa cgccagtttg tccagatctt gcgttttctc caggatttcg
     7981 tgtgccagcc gaaccagcgt ccgatcgatc tcggaagcgc tcatgagttg agtcttctcg
     8041 cgagtgggcg tcataactcg ggcagtgcca aacgcttcag gttagcacag cgtttgagtg
     8101 ccgggaaccg gggcacccac gccccccttg tctcaaggat ggccgaactc gtgggcccgg
     8161 ggaatcagct cgaaatagat cgcatccgct atcggcgtat gccagtagcc ggcgagctgt
     8221 gcatatgcca caacgccgag aaaaacggcg cacacggcag ccgccatcgc ccacgcgggc
     8281 aggacgcgtt tgcggggcag gctcatatgg agagcgtcct cggccggaca caccgctacg
     8341 cactccaggc agccggtgca ttctaccgat tggaccgtaa tgagccggtc taccggcaac
     8401 agcgaagggc aggccttggc gcatttagcg cagtcgatac aggtttccgg attgcggcgg
     8461 atgcgcgcgg gactgaaaaa tgccgccaga cccatcaacg ctccgtacgg gcagagataa
     8521 cggcaccaga agttctgtac gaataccgag agaatcacca gaaccgccaa cgtgatggct
     8581 gaagtcattc caacgaaacg gaaaaaattg agcatcttca cgtcggagat gaggccgtat
     8641 g
//
LOCUS       DASTMK010000372        23527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_81692, whole genome shotgun sequence.
ACCESSION   DASTMK010000372 DASTMK010000000
VERSION     DASTMK010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 23527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23527
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81692"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFQ79_16180"
     CDS             <1..922
                     /locus_tag="VFQ79_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU0141261.1"
                     /translation="AFAVLDREKVDLIIVDFVLPQISGPDLCRRLKSNRKTQFIPIVI
                     LTSVQGVENEINGMVSGADEFLTQPLHGDVVRARIRAMLRNKAAVDSLEEAESILFAL
                     AQAIEHRDSYTAGHCQRLAAYSSAIGIAMGLPRTSIIALHRGGYLHDIGKIGIPDSIL
                     YKQGPLTVEEWEIMRTHTIRGEQICLAMKTLAPVLPIIRHHHERYDGSGYPDGLRGTE
                     IPLLARILQVSDIYDALTTARPYKPAFSSARAMAILEEECQAGWRDPDIVQVFSEVLK
                     NAPVGPEPVAWDEGWIVQQSLQNLHRELSR"
     gene            1001..2242
                     /locus_tag="VFQ79_16185"
     CDS             1001..2242
                     /locus_tag="VFQ79_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141262.1"
                     /translation="MNRRQLLVAAGAPAAFAAMPQKEPGGRGLQKKLTGFAIRPDYLT
                     EAEHIPEFWVSTFDDVQRFLTRSVRKGTVRTIGTTAGGRPIRAVFYGKPRSGAGTSTF
                     SGALGYGDVRAWVGPDYEKKVYWGMAAVHGGEFEGIVGMVNLLAVLETGADLRGKRWP
                     ELSAAAAALDRIILLPIVNGDGRSRVPLRMGSHRGSDYTVAEYFNTGGRPDGSLIGWP
                     QCKEYIPLDFSKTQFPGGYPNDAGVNIQHDDFFGNPQPETRALFTLAASERPDLTMNM
                     HTGATFVHPLRSFIEPALHSSFEGVYRGIMTALTKAGLQGTDDPAKEADPNRERLSVF
                     NLDSAINMHCGALTVLVESPSHTFSTAKRQGQPFLHTPDHLLDAQLICHQQAMQFLVE
                     TGGRWRWTPNPRSRWSAPSAG"
     gene            complement(2239..2808)
                     /locus_tag="VFQ79_16190"
     CDS             complement(2239..2808)
                     /locus_tag="VFQ79_16190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0141263.1"
                     /translation="MKRIARFLAAGIVFCGIASADPIDFIYDFAFRTSGGISHIQFRF
                     TPPLLLHLFGPPPGPKLGLFPGSVTRITAGSFSGGEYFLDRFLFNDPVTSNGTVLPAL
                     ALRDAAIPGQPWFNTRDAAQALKLFPGTYRGKMTAVDFNTGKPAPQWNSGFTITATPE
                     PASVVLLATTGVVIGLIALRRRRGRNHHV"
     gene            3006..3800
                     /locus_tag="VFQ79_16195"
     CDS             3006..3800
                     /locus_tag="VFQ79_16195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0141264.1"
                     /translation="MIDDPRFPMRPEVATCPKNFARHGYSAEFLLDQMRLYRHDHVVI
                     SHVCYYGRDNSYAVHCVKTYPGKFSGYGLLVGYRLYSPGDKENPSRLRRLIKDDGLIG
                     LRLSPIYDRDVVWLNDPVCYPLWKNAEELGAVFHIFLAPHQVKQVADMAERFPGVTVV
                     IDHIAMIDIAASDSDGFGPLLELAKFPNVYIRTSLHNPSKTKVMPYRDVWPFLKRLYH
                     RYGAKRLVYANFFEYVIMKDLIPFFTAEDKEWILGRTAMKIYRIRL"
     gene            3957..5333
                     /locus_tag="VFQ79_16200"
     CDS             3957..5333
                     /locus_tag="VFQ79_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141265.1"
                     /translation="MDYGFKNIALRVAPKRFESWEDFVRETPEYSIGLEVADDTPGHR
                     GHHVHFDHHSGVIREATMSAALQAYIAVRQGRLMDRWLRKRKPVPVYVWNADQDVCLA
                     AFVLENHELLERSEGSPLLRWIVQYNNKIDVCGGIYPVNLHELEENHFTWVFEPYRQQ
                     RMRGKGGQNDEALVTDTIAQVCERLRRLLDGTAETAPITAKPEILYNSPYNFVIADEK
                     GDPHSRLVLAAQGYTNLISRVCTRPSGRFTYSLIRGSSYDEDTFQIGKLIDAFQAAED
                     HPDVKIWGGSNLAAGSDSEIGSSLPWTALRDIAEPIVRDAFLTNMSQSLPANEGTRSG
                     VLLVSPPGCYSGIQQHLSDCGLEVFAVADCAEARKVLSMHYQIAAVFTPVLLPDGNFR
                     DLVEMAAQRTNPVPVIACLPELDGGWVDLLESGAFQVVSEQCELQEIQKVVNSIVGCR
                     QYAIETAV"
     gene            complement(5339..6151)
                     /locus_tag="VFQ79_16205"
     CDS             complement(5339..6151)
                     /locus_tag="VFQ79_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0141266.1"
                     /translation="MAIAESKRRAGAASGEEERLKSRALAKGLEMLERLAQCSHPLGL
                     AELAHAVSLGKPSAFRLLQTLVATGYVRQREDSIYELNRVWVPALTQDWQLRLVAAAR
                     PEMEALGMELAETVTLAVLMVDHIRVVETRESPQHIRMSNYPNRILPPYASSLGKAIA
                     AFQPPERLQQLFQVYGIYATTENTITDPAAIRQELAGVRERGFSREFEETVKGGCCFA
                     APIAEKNGAVEAAISVSLPTARLTPALEKRIPSLVMATAHRIEKRMRQSFKE"
     gene            complement(6231..7643)
                     /locus_tag="VFQ79_16210"
     CDS             complement(6231..7643)
                     /locus_tag="VFQ79_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141267.1"
                     /translation="MYDPVDMFYRNVLLLIVGATALCAAPETRWQRWETLQRENLTSI
                     TAWLKKKNPALSKFSVHDKRSIDGTYAVMVVTGFDEARQQPFDPWAGVFVVYGKTNQV
                     YMVLDIRPAPQCCGPQLRNATGEFVYVDWIGDYGLYGGTQKYLYHLATRRAPERYRFH
                     RFSVKTVQPRPDHIAFFGSYSASSEMSTETLPGSIALVHRMSDGQWHAREEPAVVLQE
                     TEQATEIAGIPAPVLNAVRLPNGSLPGVERILKTKRGLWLYVAPGELPCCGYQKSGVY
                     VVNSRGAAQFYPVPVPTAALCNRLRRSTGEVAFTPGPSSDGLRNSIGPYAFDGAKLWF
                     ANYFYDGEGLSGVGAVGSFDPDSRRYEMRYLPEIAPWSGSALLLDSGSIWIGLMRQPE
                     GAAYGGGVLRFGTQSGLARKYPINDYVATITRDGSTLYFGTAHGVYVYDTDKNSLAHV
                     RVEPGPGGPLEVVTLNIETE"
     gene            complement(7665..8633)
                     /locus_tag="VFQ79_16215"
     CDS             complement(7665..8633)
                     /locus_tag="VFQ79_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141268.1"
                     /translation="MIAIVVALIVAAAALLLWRSIPTPEISTVTGAVLARDPDPHKQR
                     PIPNAIVIAGAGGRNAKAVSDGKGLFRLRLEPPVPAGEQISLRVEHPDYRPFTTGTAA
                     GDEIQLLRLTPKAPAPVPPPAQKQVAIANIRVRYATKATNTVNVGSVVRTFDILNRGN
                     VPCEGQPPCSPDGKWKAMLGSLSINTGEDNKYFRNARVSCIAGPCPFTSIESDGFSRG
                     GRAISVVVRNWSDRVTYVLEAEVEQTIQTDLIRHTYPVMFGRSLNFTLPAQAKGPSIE
                     AEVDGAEIAFPLGPELRLSWAACRLELQPDGTQVYRCELKPGFRFG"
     gene            8725..10080
                     /locus_tag="VFQ79_16220"
     CDS             8725..10080
                     /locus_tag="VFQ79_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0141269.1"
                     /translation="MSFGTEHANVVSSRREALRRIAILTGASATANRLFSLAETTEPQ
                     TGEPDLIAGQAALSPEDQAFLDELEHAAFLYFWEQADPNTGLVKDRCNVRSGNDTGTV
                     ASIAATGFGLTALCIGHSRGFVPLADVRGRVIAALRFLYEKMPAHRGFFYHWADVHSG
                     ERTWNSEISSIDTAILLCGVLTCRQHFRYREISRLATLIFNRVNWTWLSADTSLLPHG
                     WTPELGFLQYRWDYYSELMMMYLLGLGSTTHPLPVQTWHAWKRLTFEYDGLRYIGSYA
                     PLFVHQYSQAWFDFRGTRDRYANYFQNSTIATQVHRRFCLELSKRYPHFTEDLWGITA
                     SDSRNGYVVWGGPPEMGPIDGTVVPSAAGGSLAFLPRETLRVLKHIRSRYGERTWSRY
                     GFVNAFNPMTEWYDTDVVGIDTGVTLLMAENLRTGFVWDAFSRNPEVVAALKRAEFTE
                     G"
     gene            complement(10146..10712)
                     /locus_tag="VFQ79_16225"
     CDS             complement(10146..10712)
                     /locus_tag="VFQ79_16225"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0141270.1"
                     /translation="MHILMIGGTRFIGAHVTRRLHDAGAEIVAFHRGSSTNPILPDIE
                     HVLDPSAEYPVTHFPERLHREWDVVVHMVAMGEADAGAAVRTLQDRTRRLVLISSCDV
                     YRAYGRLTKTEPGPPDPVPLKEDAPLRSVLYPYRGMEAQLGTFAHDYDKILAEKAVQS
                     AVGLDWTILRLPKVYGPEDNADLSKGRP"
     gene            complement(10834..12198)
                     /locus_tag="VFQ79_16230"
     CDS             complement(10834..12198)
                     /locus_tag="VFQ79_16230"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEU0141271.1"
                     /translation="MDKPVPREITALLSDERLILRETQRAVTPFGGIAVFISFLGKIG
                     LVEAVRQHMPIRFKSPNQIQPTFTFTAFLVSVLVGARRFAHVSLLRGDRALHALLGIT
                     RFPTDDTIRNLFRRFGMGQVQRLFEPLAEWQMQRLPARDDGYTLDLDSTVFERYGKQE
                     GSLKGHNPRKHGRPSHHPLLAVMNEAHFLLHGWLRSGNCGTSRGVEEFLKEALALWGQ
                     RQKIRLLRADSGFFDDKLLSFLEQRLLPYIVVARLTGWVKRAAQRVEQWTMLDDDYAA
                     GEFQLQLHGWKTERRFVVLRERVREGRNSVGRKLIDVPGYTFRVFLTSCSDPPQEIWR
                     GYNQRADMENRIAELKQDLGADGFCLNQFFATEAAFRAVLLLFNLLAEFQRAVGLPVY
                     RQPATIRTQVLTCGAILGRAGRRLVIHLSESWGGLKTRIPLLDSLSKWEIPTSPKLES
                     AVVT"
     gene            complement(12246..14462)
                     /gene="bglX"
                     /locus_tag="VFQ79_16235"
     CDS             complement(12246..14462)
                     /gene="bglX"
                     /locus_tag="VFQ79_16235"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HEU0141272.1"
                     /translation="MTLALQTSLPADIEKRIDALLARMTLEEKLGQMSQATSMRNPIA
                     EEIKQQIRQGRWGSFLNAGLPEDRAEAQRIAIQESRLGIPLLFGRDVIHGYRTVFPIP
                     LGQAAAWNPGLIERAAAIAAREASTEGIHWTFAPMLDVTRDPRWGRVAESLGEDPYLT
                     GRLGSAMIRGFQGGSLREAGRIAACAKHFAGYGAAEAGRDYNSTFIPENQLHEVYLPP
                     FHDAIAAGVATFMTAFNALNGVPASGNRFLLDRILRKEWGFTGVVVSDYESVLEMVQH
                     GFAAGPAEAAQKAVTAGVDMEMVSTTYFDNLRRLVEKQNVSIASIDAAVRRILRLKYQ
                     LGLFDAPMRPAASVQTANDARGIAKELATQSLVLLKNDNDVLPLSPAVKRIAVIGPLA
                     NSPVDQMGSWVMDARPEEVETPLTSLQRKFGAGRIRYVAALRNSRDTSREQFNAAIEA
                     AKASDVVLLLLGEEQILSGEAHSRAFLNLPGAQEGLAAEIAATGTPIVTIILAGRPLT
                     FQNVAAKSAGVLYAWHPGTMGGPALLDVLTGDVSPSGKLPISFPRAVGQIPIYYSHLN
                     TGRPASPGELGIPLGSPVNPEGFTSKYLDVDFTPEYPFGFGLSYTTFEYSKPRISTAE
                     LTPGSSITVSAEIANTGKRAGEEVAQLYIRDPVASVARPVRELKGFSRVRLNPGEKRT
                     VSFTLTPRDLSFYNESMELVTEPGEFQVWIAPDSASGSDVRFRFRAGTKPENRSLK"
     gene            complement(14547..15287)
                     /gene="rsmA"
                     /locus_tag="VFQ79_16240"
     CDS             complement(14547..15287)
                     /gene="rsmA"
                     /locus_tag="VFQ79_16240"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU0141273.1"
                     /translation="MGRRLGQHFLVRGSVLERIARAACPEPAPRVIEIGPGKGALTAH
                     LVSKAGEVIAIELDEELALRLRSRFPDITVISGDVLTTDLAQWGAAVVAGNLPYYITS
                     PVIEKVLALGPLLTRAVFLIQKEVAQRLVAEPGSRDYGYLTVAILIYARAEYLFTVPP
                     AAFHPPPKVESAVVRLTPHPPPVEHPEELLAFVGQCFRQKRKTIRNNLLPGYGHQVDA
                     WPEAALRAEQLTLEQFAAMHRRLRGVER"
     gene            complement(15454..16140)
                     /locus_tag="VFQ79_16245"
     CDS             complement(15454..16140)
                     /locus_tag="VFQ79_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0141274.1"
                     /translation="MKCHEVEKNLSAYLDGFLPQAESQTISLHLTHCPKCTVRCEQTD
                     VLRSRLRALPAQTPPASLTFSLRVIGSKEQTRRRKSATLSNIWEKWAASTRLWRDNLM
                     RPLAIPLAGGLASAVFLFSMVVPNVATYQSFTGNDVPTAFYTEATVKSTGPFTFGDDD
                     IIVELTVDEQGRMVDYSFPSKVSENPELLRIIENNLLFTTFTPATTFGQPTAGKVRVS
                     FSKSHIEVKG"
     gene            complement(16137..16844)
                     /locus_tag="VFQ79_16250"
     CDS             complement(16137..16844)
                     /locus_tag="VFQ79_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0141275.1"
                     /translation="MADTTIGYHFGSHVAAYDLAPCADCHQQEEELLVEGLQAGENEA
                     YEALISQFQQPVYNLVHRLLTDTSDTCDVVQDVFLKVFRNIGSFRRQSSLKTWIYRIA
                     VNEAHNQRRWFTRHRRQEVGLESDEERNRNYQETLCDSSRSPFEETLSHETQALIERA
                     LAQLNPTFRAAVVLRDIEGLSYEEIADVLQVSLGTVKSRILRGREALRKTLAGQLEPG
                     PAWSWTPQPALGPRCEP"
     gene            complement(16958..17407)
                     /gene="nusB"
                     /locus_tag="VFQ79_16255"
     CDS             complement(16958..17407)
                     /gene="nusB"
                     /locus_tag="VFQ79_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU0141276.1"
                     /translation="MPSRHSSRERAVQILYQLDMRKQEVDEAIAAFYGTLSSSDEHPA
                     NIEEQDAFMEELVRGTVSQLADIDLLIAGHSANWRIERMPAVDRNILRCAVYEMRYLG
                     TPAPVAIDEALELARRFSGDDSVAFVNGVLDAVSKSTTIRTKESDSV"
     gene            complement(17414..17893)
                     /gene="ribE"
                     /locus_tag="VFQ79_16260"
     CDS             complement(17414..17893)
                     /gene="ribE"
                     /locus_tag="VFQ79_16260"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU0141277.1"
                     /translation="MPHRIIEGNLSAQGLKFAILVSRFNSIITERLLAGALDALARTG
                     CAEEDIEIVKVPGSWELPMAANEIAAQKKHDGIICLSAIIKGETPHFDYIAAEAAKGL
                     AQASLQHGVPVAFGVLTTGTLEQAIDRAGAKSGNKGFDAAMTAVEMGNLVRQLRAGR"
     gene            complement(17897..18682)
                     /locus_tag="VFQ79_16265"
     CDS             complement(17897..18682)
                     /locus_tag="VFQ79_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0141278.1"
                     /translation="MSYSHIRFEAGADRIALVTINRPEKRNALNAHVMEELADAFERA
                     RSDATIGALLVTGAGDKAFVAGADIGELSEMTPVEARQAALRGQQIFRKLETLGKPSV
                     ACINGYALGAGLELAMACTLRVACPEAKLGQPEVKLGLLPGYGGTQRLPRLVGRGRAL
                     DLLLSGEPVDAEEAHRIGLVNYVTPRENLQPFARGLLEKVLTNGPIAISLILEAVDVG
                     TSAGLEEGLRFEAAAFGLIADTQDRREGTRAFLEKRKAVFAGK"
     gene            18718..20526
                     /gene="lepA"
                     /locus_tag="VFQ79_16270"
     CDS             18718..20526
                     /gene="lepA"
                     /locus_tag="VFQ79_16270"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU0141279.1"
                     /translation="MDSAFIRNFSIIAHIDHGKSTLADRLLEVTGALSQREMMEQVLD
                     SMDLERERGITIKAHAVRLNYQASDGETYQLNLIDTPGHVDFSYEVSRSLQACEGALL
                     VVDASQGVEAQTLANAYLALHHDLEIIPVINKIDLPAADPERIRAQIEQVVGLDASDA
                     ILVSAKQGVGVEDVLEAVVHLVPPPKGSPESPLRALVFDSWFDPYRGVITLLRVVDGT
                     LRTGQKIRLWSNGKSFEVEGLGYQSPKAIPAEQLSAGEVGYMFANIKTVSDAQIGDTV
                     TDDAKPAAQPLPGFEQIKPMVFAGLYPVESHEHGVLREALEKLRLNDSAFSFEPESSV
                     ALGFGFRCGFLGLLHLEIVQERLEREFQIDLITTAPGVRYRVVLRDGDVVEVDSPTKF
                     PDPAKIDKIEEPIIEATVITREEFLGEILKLLDEKRGVQKKFEHIGSDRVLLTYEIPL
                     NEVVLDFYDRLKSASRGYASLDYHLAGYRESPMVKLDVLVAGEPVDALSIIVHKDFAH
                     ERGKALIERLRKLIPRQMFEVALQAAIGNKVVARENIPAIRKNVIAKCYGGDITRKRK
                     LLEKQKEGKRRMKRVGRVEIPQEAFLAVLRVGGNQT"
     gene            20561..22183
                     /locus_tag="VFQ79_16275"
     CDS             20561..22183
                     /locus_tag="VFQ79_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0141280.1"
                     /translation="MRKSFLCLLSLVLALVASAAPPQAAPQGTPKLILTLVVDQFRYD
                     YLTRFRSDYNGGLARLLTHGAVFSNAHYEHFPTVTAIGHSTILSGATPSISGIVGNEW
                     YDRELNKQVTSVSDSTVQLLGGRAGEAGASPRKLLVSTIGDELKMSGKGTPRVIGMSI
                     KDRSAILPAGHMADAAYWFDDVTGNFVSSTYYSADLPAWVKAFNSGRAVDRYITKQWS
                     SNSGTAGAAPQVFATMPPKPDSAYFKLLERTPFGNEVLELFAEHAIEAEQLGQRGTTD
                     LLSVSFSSNDYVGHGVGPDAPEVRDISIQTDRLFARLLDFVDRKVGLDNVLVIFTADH
                     GVSPLPELMKQRKMPGGRMSARVVLDGVQKAIEAKYGEGNWVIGKSGPAPYFNYQLIR
                     ERKLDLAEVQRVAADAVRNIPHIARVYTREQLKNGQILEDRIGRRVRNGFNNERGSDL
                     FIVVEPYWLFEERGTSHGTPYNYDSHVPVIFMGRGIKAGQYNQPIAVNDIAPTVATFL
                     EIETPSGAEGRVLDEMLTAPAPGVHLSSYVEK"
     gene            22200..22493
                     /locus_tag="VFQ79_16280"
     CDS             22200..22493
                     /locus_tag="VFQ79_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU0141281.1"
                     /translation="MDIRPLHDHVLVKRIEEAETVSGSGIIIPDTAKEKPQQGEVIAV
                     GNGKLLSNGERSVIDVKPGMRILFTKYAGSDIKSAGEEYLVLREDEILGVLEQ"
     gene            22692..23213
                     /locus_tag="VFQ79_16285"
     CDS             22692..23213
                     /locus_tag="VFQ79_16285"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU0141282.1"
                     /translation="MTRASFRLIVMGAALLASASLAGAQTKVAVVNLQRAVLETADIK
                     KAQADLEARYRPQQQEMEKLQKEVEALSQQLQTMRGKLTQQGEQDLVVQGQRKQRELQ
                     RLTEDLQGEVNRDREEILARVSQQMSEVVGKLATQKDLDIVVDVTNTVFFKPALDLTK
                     EAVAAYDAAHPVK"
     gene            23223..>23527
                     /locus_tag="VFQ79_16290"
     CDS             23223..>23527
                     /locus_tag="VFQ79_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141283.1"
                     /translation="MSGYLEAYGAGDEKRSKIIRWTAITVVAVLVIGLFLYFGFRNYR
                     EDRIANQFVEFLKTQDYQAAYRLWGCTPESPCPDYSIEKFMEDWGPKSPYGSPANAEV
                     "
BASE COUNT         4747 a         6898 c         7142 g         4740 t
ORIGIN      
        1 tgccttcgcg gtgctcgacc gtgaaaaggt cgatctgatc atcgtcgatt tcgtacttcc
       61 gcagatcagc ggccccgacc tctgccgccg gctgaagagc aatcgcaaga cgcagttcat
      121 tcccatcgtt attctgacca gcgtgcaggg cgttgaaaac gaaatcaacg ggatggtgtc
      181 gggcgccgat gaattcctga cacaaccgct tcacggcgat gtcgtccggg cacggatcag
      241 ggcgatgctg cgcaacaagg cagccgtcga ttcgctcgaa gaagccgaaa gcattctgtt
      301 tgcgcttgcc caggccatcg agcatcgcga ctcctacacc gccggacatt gccagcggct
      361 ggccgcatac agctcggcaa tcggcatagc gatgggactc ccccgcactt ccatcattgc
      421 gctgcatcgg ggtggctatc tccacgacat tggaaaaatc gggatacccg attccattct
      481 ctacaagcaa ggtcccctga cagtcgaaga atgggagatc atgcgcacgc acacgattcg
      541 cggagagcag atctgcctgg cgatgaagac cctggcaccg gtattgccga tcatccggca
      601 tcatcatgaa aggtacgacg ggtcgggata tccggacggg ctgcggggca cggaaatacc
      661 gctgctggcc cgaattctcc aggtgagtga catctacgac gcgctcacaa ctgcacgacc
      721 ctacaaaccc gcgttttctt ccgcacgcgc catggcgatc ctcgaagagg aatgccaggc
      781 cggctggcgc gatccggata tcgtgcaggt gttttccgaa gttctgaaga acgccccggt
      841 ggggccggag ccggtggcct gggatgaagg ctggatcgta cagcagtcct tgcaaaacct
      901 gcatcgggag ctttcccggt aagcatttaa ctgctccgcc tgtgccgggc gggagcggct
      961 cacgttattt ccgcactaca ctggacacag gacttcctcc atgaatagaa ggcaactact
     1021 tgtggctgcc ggtgcgccgg ccgcctttgc cgccatgcct caaaaggaac cgggcgggcg
     1081 gggactgcaa aagaagttga cgggattcgc catccgcccg gactatctga cagaggcgga
     1141 gcacatcccc gagttttggg tcagcacctt tgacgacgtg cagcgcttcc tcacgagaag
     1201 cgtgcgcaag ggtacggttc gtacgatcgg caccaccgcc gggggacgcc ctatacgcgc
     1261 ggtgttttac ggaaagccac gctcgggagc aggaacctcc accttttccg gcgcgctcgg
     1321 ctatggggac gttcgcgcct gggtggggcc cgattacgag aagaaggtgt attggggcat
     1381 ggcggcggtt catggcgggg aatttgaagg catcgtcgga atggtgaacc tgctggcggt
     1441 gctcgaaacc ggagccgacc ttcgtgggaa gcgatggccg gaattatccg ccgcggcagc
     1501 cgccctggac cggatcattc tcctgccgat tgtgaacggc gacggccgct cgcgcgttcc
     1561 gctgcgcatg ggctcacatc gaggcagcga ttacacggtg gccgagtact tcaataccgg
     1621 tggccgtccc gatggctccc tcatcggatg gccacagtgt aaggaataca tcccactcga
     1681 tttctcgaaa acgcagtttc cggggggcta tcccaacgac gccggcgtga acattcagca
     1741 tgacgatttc ttcgggaatc cgcagccgga gacacgcgct ctgttcactc tcgcggcgtc
     1801 cgaacggccg gatctgacca tgaacatgca taccggcgcg accttcgtcc atcccttgcg
     1861 ttccttcatc gagccggctc ttcattcatc cttcgagggt gtataccgcg gcatcatgac
     1921 cgcgctgacc aaggcgggcc ttcagggtac cgatgacccc gcaaaggaag ccgaccccaa
     1981 ccgcgagcgt ctttcggtgt tcaatctcga ttcggcaatc aatatgcatt gcggcgctct
     2041 tacggtgctg gtggagtctc cctcgcacac gttctccacc gcgaagaggc aagggcagcc
     2101 ctttctccac acgccggacc atcttctgga cgcgcagttg atttgccatc aacaggcgat
     2161 gcaatttctg gttgaaaccg gcggccgctg gcgctggact ccgaatcccc gatccaggtg
     2221 gagcgcgccg tccgccggct aaacgtggtg atttctgccg cgccgccgcc gcagcgcgat
     2281 caacccgatc acgacgccgg tagtggccag cagaaccacg ctcgccggtt caggtgtagc
     2341 ggtaatcgta aatcccgaat tccattgcgg cgccggtttg ccggtgttga agtcgacagc
     2401 ggtcatcttg ccccggtacg ttcccggaaa cagcttcagc gcctgggcgg catcgcgcgt
     2461 attaaaccac ggctgtccgg gaattgcggc atcgcgaagc gctagcgccg gcaacaccgt
     2521 gccgttcgat gtaaccggat cgttgaaaag gaagcggtcg aggaaatatt ccccgccgct
     2581 gaaagatccg gcggttattc gcgtgaccga gccgggaaaa agcccgagtt tcggtccggg
     2641 tggaggtcca aagagatgca gcaatagcgg aggagtaaac cggaattgga tgtgagagat
     2701 tccaccgctg gttcgaaagg cgaaatcgta aatgaaatcg atgggatccg cactggctat
     2761 accacaaaat acgattccgg ctgccaggaa acgagcaatc cttttcacga ttttacccct
     2821 tgcgcaaagt ttacatatct ccggaatttt tgccaatagg attggtacca ggaggaccag
     2881 tcccgccccg gctgtgataa gatccgccga taacgcgccg ccacctgctg ataaccggaa
     2941 ccgcgggcct gcttcccgcc gcttccgccg ctgctcccgt cgttaacgcc gccgaacacg
     3001 cctgggtgat cgacgatccg cgatttccaa tgcggccgga agtggcaacc tgccccaaga
     3061 atttcgcccg gcatggttat tcggcggaat ttttgctcga tcagatgcgg ctttaccgcc
     3121 acgaccatgt cgttatctcg catgtttgct actacgggcg ggacaactcc tacgccgtac
     3181 attgcgtgaa gacgtatccc ggcaagtttt ccggctacgg cctgctggtt ggctaccggc
     3241 tgtattcgcc cggcgataaa gagaacccgt cgcggctgcg gcggctcatc aaagacgatg
     3301 gtcttatcgg tttgcgcctg agccccattt acgaccgcga cgtcgtctgg ttgaacgacc
     3361 cggtgtgcta tccgctatgg aaaaacgccg aagaacttgg ggccgtgttt cacatttttc
     3421 tggcgccgca ccaggtgaag caggtcgccg atatggcgga gcgctttccc ggggtgacgg
     3481 tggtcatcga ccacattgca atgatcgata tcgccgcttc cgacagcgac ggattcgggc
     3541 cgctgctgga attagcgaaa ttccccaatg tgtatatccg cacttccctg cataacccgt
     3601 ccaaaacaaa ggtgatgccg tatcgggatg tctggccttt tctgaagcgg ctgtaccacc
     3661 gctacggagc gaagcgcctg gtctacgcga atttcttcga gtacgtgatc atgaaagacc
     3721 tgatcccttt ctttacggcc gaagacaaag aatggattct aggccgcacc gccatgaaga
     3781 tctaccgtat ccggttgtag gcttcacaga ccgggtggcg attgactgcc cggcccgaca
     3841 cggcgacaat gagggcggga aggttgtggg gacagccggt gtgagttgct gttccgggca
     3901 accggggttc cccgttccga cgggcaatgc aggagccttt ttcccccggg atgaagatgg
     3961 actacggttt taagaatatt gctttgcggg ttgccccgaa gcgttttgaa agttgggagg
     4021 acttcgtcag ggaaacgccg gagtactcca tcggcttaga ggtagcggat gacacgccgg
     4081 ggcaccgcgg ccaccacgta catttcgacc atcactccgg agtgatccgg gaggcgacca
     4141 tgagtgccgc gctacaggcg tacatcgccg tgcgccaggg acgtctgatg gaccgctggc
     4201 tgcggaaacg gaaaccggtt cccgtttacg tttggaacgc cgatcaggat gtctgcctgg
     4261 cggcatttgt tctggagaac cacgaactgc tggaacgcag cgaaggttcg ccgttactgc
     4321 ggtggattgt gcagtacaac aacaagatcg acgtttgtgg aggtatctac ccggtcaacc
     4381 tccacgaact ggaagagaac catttcacgt gggtgtttga gccataccgc cagcagcgaa
     4441 tgcgaggcaa gggcggccag aacgacgagg cgctggtgac cgatacgatc gcgcaggtct
     4501 gcgagcgttt acggcgtctt ctggacggaa cggcggagac ggcgccgatc acggcaaaac
     4561 cggagatcct ttacaactct ccatacaatt tcgtcatcgc cgatgagaag ggcgatccgc
     4621 actcccggct ggtgctggcc gcgcaaggtt acacgaacct gatcagccgg gtttgcacgc
     4681 gtccgtccgg ccgcttcacg tacagcctga tccgcggctc gtcctacgac gaggacacgt
     4741 tccagatcgg gaaactcatc gatgcctttc aagccgccga ggaccatccg gacgtgaaga
     4801 tctggggcgg gtcgaatctc gctgccggct ccgacagcga aatcggtagt tcgctgccgt
     4861 ggaccgccct gcgggatatc gcggagccga ttgttcgcga tgcgttcctg acgaacatga
     4921 gtcaatcgct gccggcgaac gaaggtacgc gctcgggggt cctgctggtc tccccgccgg
     4981 gctgctattc cggaattcag cagcaccttt cagactgcgg cctggaggtc ttcgcggtcg
     5041 cggactgcgc ggaagcgcgc aaagttctgt cgatgcatta tcagatcgcc gccgtattta
     5101 cccctgtgct gctgcccgac ggcaatttcc gcgacctggt ggagatggcg gcgcaacgca
     5161 cgaatccggt accggtgatc gcatgcctgc cggagctcga cggtggctgg gttgatctgc
     5221 tcgaatcggg ggcttttcaa gtggtcagcg agcagtgcga gcttcaggaa atccaaaagg
     5281 tggtgaattc catcgtgggt tgccggcagt acgcgatcga aaccgccgtc tgacggtgtt
     5341 attccttaaa gctctggcgc atccgtttct cgatgcggtg cgcggtggcc atcacgagcg
     5401 agggaatccg tttttccagg gcgggagtca gccgcgcggt tggcaacgag acactgatgg
     5461 cggcttcaac ggcgccgttc ttttccgcaa tgggggccgc aaaacagcag ccgcctttta
     5521 ccgtctcctc gaactcgcgg gagaagccgc gctcgcgcac accggccagt tcctgccgga
     5581 tggccgccgg gtcggtgatg gtgttctccg tagtggcgta gatgccataa acctggaaca
     5641 gttgctggag ccgctccggc ggttgaaacg ccgcgatcgc ctttcccagc gaagaagcat
     5701 agggcggcag aatgcggttc gggtagttgg acatgcggat atgctgcggg ctttcgcgag
     5761 tctccacgac gcggatgtgg tccaccatca gcaccgccag ggtcacggtc tccgccagtt
     5821 ccatcccgag cgcttccatt tcaggccggg ccgccgcaac cagccgtagc tgccagtcct
     5881 gtgtcaacgc aggcacccat acacgattca actcgtatat actgtcctcc cgctggcgga
     5941 cgtaaccagt ggcaaccagt gtttgaagca accggaatgc cgacggcttt ccaaggctca
     6001 ccgcgtgggc aagctccgca agcccgagcg gatgcgagca ctgtgccaga cgttccaaca
     6061 tctccagccc cttggcgagc gcgcgggatt tgagccgttc ctcctcgccg gaagcagctc
     6121 cggctcttct tttggattcg gctattgcca tgtgtttacc gattatacgt gaatcttacc
     6181 cagcaaatgc agctttcgga agaatatcga aacccgattt cacttgtggt tcattccgtc
     6241 tcaatattga gtgtcacgac ctctagcggc ccgccgggcc ctggctcgac acgcacatgc
     6301 gccagtgagt ttttgtctgt gtcatagacg tacacgccgt gagcagtgcc gaaatacagc
     6361 gtggaaccat cccgtgtgat ggtagcgaca tagtcattga tcgggtattt ccgcgccaaa
     6421 ccggattgcg tgccgaaccg gagcaccccg ccgccgtatg ccgcgccttc aggctgccgc
     6481 ataagcccga tccagatcga accggaatcg agcaacagcg cggagccgga ccatggcgcg
     6541 atttcgggaa gatatcgcat ctcgtagcgg cggctgtcag gatcgaagct gcctacagcc
     6601 ccgactccgg atagtccctc tccgtcgtag aaatagttgg caaaccaaag cttcgctccg
     6661 tcgaatgcat agggcccaat cgagttcctc agaccgtccg aagaggggcc gggtgtgaat
     6721 gcaacctcac cggtgctgcg acgcagtctg ttacagagcg cggccgtggg aaccggaacg
     6781 gggtagaatt gcgccgcacc tcgcgagttc acgacatata ctcccgattt ctgatagcca
     6841 caacacggta gttccccggg cgccacatag agccataggc cgcgctttgt tttcaggatt
     6901 cgttccacgc ccgggagact gccgttcggt agacggacgg cattgagcac gggcgccgga
     6961 atgccggcga tttccgtcgc ttgttccgtt tcttgtaaga ccactgcggg ttcctcgcgg
     7021 gcgtgccact gcccgtcgct catccgatgg accagcgcga tacttccagg caaagtttcc
     7081 gtgctcatct cactcgaagc cgagtaggaa ccgaaaaacg cgatatggtc cggccggggt
     7141 tgcacagtct ttacggaaaa gcggtgaaag cggtagcgtt cgggagcgcg ccgcgttgcg
     7201 agatgataca ggtatttttg agtgcccccg tagagtccgt agtcgccgat ccaatcgacg
     7261 tacacgaatt cgccggttgc gttgcgcagt tgagggccgc agcactgtgg cgccgggcgg
     7321 atgtccagca ccatatacac ctgattcgtt tttccgtaga ccacgaaaac gccggcccat
     7381 ggatcgaacg gctgctgccg cgcctcgtca aagcctgtga caaccatcac ggcgtaggtg
     7441 ccgtcgatgg agcgcttgtc gtgcaccgaa aatttggaga gcgctggatt tttcttcttt
     7501 agccaggccg taatgctggt gaggttttcc cgctggagtg tttcccatct ttgccaccgc
     7561 gtttcgggcg cggcgcaaag ggcggtggcg ccgacgatca acagcagcac gttccggtag
     7621 aacatgtcta ccggatcata caacttctcc cggacgcctc gccgttatcc aaaccggaag
     7681 cccggtttca gttcgcaccg gtacacctga gtgccgtccg gctgaagttc gagccggcaa
     7741 gcggcccatg acaggcgaag ttccggtccc agagggaacg cgatctccgc tccgtccact
     7801 tcggcttcaa tgctgggtcc tttggcctgg gcaggtagcg taaagttgag cgaccggccg
     7861 aacatcacgg ggtacgtgtg gcggatcaag tcggtttgta tcgtttgctc gacctcggct
     7921 tcgagtacat aggtgacgcg atccgaccag ttgcgaacca cgacgctgat ggcgcggccg
     7981 ccgcgcgaaa agccgtccga ttcgattgac gtgaacggac acggaccggc aatgcacgac
     8041 actctcgcgt tgcggaagta tttgttgtcc tcgccggtat tgatcgacag ggaccccagc
     8101 atcgctttcc attttccgtc gggtgagcag ggcggctggc cctcgcacgg aacgttgccc
     8161 cgattcagaa tgtcgaaagt acggacgacg cttccgacat tcacggtgtt ggtggccttg
     8221 gttgcatacc ggacgcgaat attcgcgatg gccacttgct tctgcgcggg cggcggcaca
     8281 ggggccggtg cttttggagt gaggcgaagc aactgaattt cgtcaccggc ggctgtgccg
     8341 gttgtgaatg gacgatagtc gggatgctct acccgcaggg agatctgttc ccctgcggga
     8401 accggcggtt ccaggcgcaa acgaaaaagc cccttaccat ccgagacggc tttggcgttc
     8461 ctgccaccgg ctccggcaat gacgatcgcg tttggaatgg gccgttgctt gtgcgggtcg
     8521 ggatcccgtg cgagaacggc gccggtgacg gtggagattt ccggtgttgg aatcgatctc
     8581 caaaggagca gggctgcggc cgccacaatt agcgccacaa caatagcgat cacagtagtc
     8641 ctgcgtgaca tagccggggg catcccataa atatagtacg atgcgtataa ggatgccaga
     8701 ttatcggggg gcgtttctca cattgtgagt ttcggaacag aacatgcaaa tgttgtctca
     8761 tcgcgccgtg aagcactaag gcgcatcgcg atcctgacag gcgcatcggc aaccgcgaac
     8821 aggctgttct ctcttgccga gaccacggag ccgcaaacgg gcgagccgga tcttatcgcg
     8881 ggacaggctg ctttgtcccc ggaggaccag gccttcctcg acgagctcga acacgccgct
     8941 tttctgtatt tctgggagca ggccgaccct aacaccggac tggtaaaaga ccgctgcaat
     9001 gtgcggagcg gcaacgacac gggtacggtc gcaagcattg ccgcaaccgg gttcggcctc
     9061 acggctctct gcatcggaca ttcacgtggt ttcgtcccgc tggccgacgt gcgcggccgg
     9121 gtgattgcag cactccggtt cttgtatgag aagatgcccg cacatcgcgg ctttttctat
     9181 cattgggccg acgtacacag cggggaacgg acgtggaatt cggagatctc gtctatcgac
     9241 accgccattc tgttgtgcgg tgtgctcacc tgccgccagc atttccggta tcgcgaaatc
     9301 agccggcttg cgacgctgat cttcaatcgc gtgaactgga cgtggctttc ggcggatacc
     9361 agcctgctgc cgcacggctg gactccggaa ctcggcttcc tccaatatcg ctgggactac
     9421 tacagcgaac tcatgatgat gtatcttctc ggcctgggct ctacgacgca tcctttgccg
     9481 gttcaaacct ggcacgcctg gaagcggctg acgttcgaat acgacggcct ccgctatatc
     9541 ggctcctacg cgccgctgtt cgtgcatcag tactcgcaag cctggttcga ttttcgaggc
     9601 acgcgggatc gatacgccaa ttatttccag aattcgacca tcgcgacgca ggtccatcgc
     9661 cgcttttgtc tcgagttgtc aaaaaggtat ccacatttca ccgaggacct ttggggcatt
     9721 acagcgtccg attccaggaa cggctatgtg gtttggggcg gcccgccgga gatgggcccc
     9781 attgatggaa cagtggttcc gagcgcggca ggcggttcgc tcgcttttct accgcgggag
     9841 acgctgcggg tcctcaagca tattcgaagc cgttatggcg agcggacctg gagccgctat
     9901 ggattcgtca acgccttcaa tcccatgacg gagtggtacg acaccgacgt ggtgggaatc
     9961 gatacgggcg tgacgctgtt gatggccgag aatttgcgaa ccgggttcgt atgggacgct
    10021 ttttccagga accccgaagt ggtggcggct ttgaagcgcg ccgagttcac cgaaggatag
    10081 tgttcgcccg gcgtcttacg cacgtccatt ctttggcgat gaagtagcca aggcagcaag
    10141 ctgcatcatg gccggccctt cgaaagatcg gcattatctt ccggtccgta aacttttggc
    10201 agccggagga tcgtccagtc gaggccgacg gcgctctgca cggccttttc ggccaatatc
    10261 ttgtcatagt cgtgcgcgaa agtgccaagc tgtgcttcca tcccgcgata gggatagaga
    10321 accgaacgaa gcggagcatc ctccttcagg ggaacgggat caggtggtcc gggttccgtt
    10381 ttggtcagtc gcccataagc tcgatagacg tcacagcttg agatgaggac caggcgccgt
    10441 gtccggtcct gaagcgtgcg aaccgcagcc ccggcgtccg cttcgcccat tgccaccatg
    10501 tgaacgacaa catcccactc acgatgcagc cgttcgggga aatgcgtcac cgggtattcc
    10561 gcgcttgggt ccaaaacatg ctcaatgtca gggaggatgg ggttggtgct ggagccgcgg
    10621 tgaaaggcga cgatctctgc tcccgcgtca tggaggcgtc gtgttacatg cgctccgatg
    10681 aagcgtgtgc cgccgatcat cagaatatgc attcctcttc tctctcgatg atagagcgcg
    10741 ccggcttact gacgattagc ctgaaatccg aagttgaacg cctgattgac ctcctgggga
    10801 ccttcgaaac gtaggtaggc gactgtccgc cgatcaggtt acgacggcgg attctaactt
    10861 cggcgaagtt gggatttccc acttcgagag actgtccagc aaggggatcc tggttttcag
    10921 cccgccccaa ctttccgaca gatggatcac caatctgcgc cctgctcggc cgaggatcgc
    10981 gccgcaagtg aggacttgag tccgaatggt ggccggctgg cgataaaccg gcaaaccaac
    11041 cgcgcgttgg aactcggcca gcagattgaa cagcagtaac acggcacgaa acgcggcctc
    11101 ggtagcgaag aactggttca ggcagaagcc atcggcaccc aggtcctgtt tcaactcggc
    11161 gatgcgattt tccatgtcgg cgcgttggtt gtagcctcgc cagatctcct gcggagggtc
    11221 ggagcaactg gtaaggaaga cgcggaaggt gtaccccggc acgtcgatga gcttgcgccc
    11281 tacactgttg cggccctcgc gcactcgctc ccgtagaacc acgaatcgcc gttccgtctt
    11341 ccagccgtgc aactggagct gaaactcgcc cgcggcatag tcgtcatcca acatcgtcca
    11401 ttgctccacc cgctgggcgg cgcgtttcac ccaaccagtc agccgggcca ctacaatgta
    11461 gggcagcagg cgctgttcca aaaagctcag cagcttgtca tcgaagaagc cggaatcggc
    11521 ccgtagcaag cggatcttct ggcgctgtcc ccacagcgcg agagcttctt tgagaaactc
    11581 ctccacgcca cggctggttc cgcagttgcc gctgcgaagc cagccgtgga gaaggaagtg
    11641 cgcttcattc atcaccgcta gcaaaggatg atgactgggc cgtccatgct tgcgcgggtt
    11701 gtgccctttg agcgaaccct cctgcttgcc gtaacgttca aaaacggtcg aatccaaatc
    11761 cagggtgtag ccgtcgtcgc gcgccggcaa acgctgcatc tgccattccg ccagcggctc
    11821 gaacagccgc tgcacctggc ccatcccgaa ccggcggaac aaattgcgga tggtatcgtc
    11881 ggtaggaaaa cgggttatcc ctagcagcgc atggagcgcc cgatcaccgc gcagcagact
    11941 gacgtgagcg aaccgtctgg ctcccaccag caccgacacc agaaatgccg tgaaggtgaa
    12001 cgttggttgg atctgattcg gcgacttgaa gcgaatcggc atgtgctgcc gcactgcttc
    12061 caccaagccg attttgccca agaacgaaat gaaaaccgcg atccccccga acggggtcac
    12121 cgcgcgttgc gtttcgcgca gaatcaaccg ctcatccgac aacagggcgg tgatctcgcg
    12181 cgggactggt ttgtccatcg cgtttgattc cattcccctg ttgtctcaaa tctttgggac
    12241 ccttcctact tcaagcttcg gttttccggc ttagtgcccg cccggaaccg gaatcgcaca
    12301 tccgagccgc tggccgaatc gggtgcaatc cacacctgaa actcgcccgg ctccgtcaca
    12361 agctccatcg attcgttata aaagctcagg tcgcgcgggg tgagggtgaa ggagaccgtg
    12421 cgcttttctc cgggattcag gcgaacgcgg ctgaaccctt tcaattcgcg gacgggccgg
    12481 gcaacgcttg ctaccggatc gcgaatatac aattgcgcga cttcttctcc cgcccgtttt
    12541 cctgtattcg cgatctccgc cgacaccgtg atgctggaac cgggtgtcag ctccgcggtt
    12601 gaaatgcgcg gcttcgagta ttcgaacgtc gtataggaaa ggccgaagcc gaagggatat
    12661 tccggcgtaa agtccacgtc gagatacttc gaagtgaagc cctccggatt gacgggacta
    12721 cccagaggaa tgccgagctc tcccggactc gcgggcctgc cggtgttcag gtggctgtaa
    12781 tagatcggaa tctgtccaac cgcgcgagga aaggagatcg ggagtttgcc ggagggactc
    12841 acatcgccgg tcaatacgtc gagcaacgcc ggcccaccca tcgttccggg atgccatgcg
    12901 taaagcacac ccgccgattt cgccgccacg ttctgaaacg tcaaggggcg ccccgcgagg
    12961 ataatggtca ctatcggcgt tccggtcgcg gcgatctccg cggccagtcc ctcctgcgca
    13021 cctggaagat tgaggaaggc gcgggaatgc gcttctcccg agaggatctg ctcctcgccc
    13081 agcagcagca gaacgacatc gctcgccttg gccgcctcga tagcagcgtt aaactgctcg
    13141 cggctggtgt ccctgctgtt ccggagagcg gcgacatacc ggatccggcc tgcgccgaac
    13201 tttcgttgga gactggtgag gggcgtttcg acctcttccg gcctggcatc catgacccac
    13261 gagcccatct gatccacggg gctgttagca agcgggccga tcaccgcgat gcgcttcacc
    13321 gcgggcgaaa gcgggagcac gtcattgtca ttcttcaaaa gcaccaggct ctgggtggcg
    13381 agttctttgg cgatgccgcg cgcatcgttg gcggtctgga cggaggccgc cggacgcata
    13441 ggagcgtcga acaacccgag ctgatacttc aagcgcagaa tgcggcggac ggcggcatcg
    13501 atggaggcta tggaaacgtt ctgcttttcg acaagccggc gtaagttgtc gaagtaggta
    13561 gtgctgacca tctccatgtc cacaccggct gttaccgcct tttgcgcggc ctcggcgggc
    13621 ccggcggcga atccatgctg caccatctcc agcaccgatt cgtaatcgct gaccacgacg
    13681 ccggtgaacc cccattcctt gcgcaaaata cgatcgagta aaaaccggtt cccgctcgcc
    13741 ggcacgccat tcagagcgtt gaacgctgtc atgaaagttg cgacgcccgc ggcgatggca
    13801 tcgtggaagg gcggcagata gacctcgtga agctgattct cgggaatgaa ggtggaattg
    13861 taatcccgtc ccgcttcggc ggcgccatat cccgcaaaat gcttcgcaca agccgcgatt
    13921 cgtccggctt cccgcaacga gccgccttga aatccgcgga tcatcgccga cccaagccgg
    13981 cctgtcaggt agggatcttc gcccaacgat tccgcgacgc gtccccatcg tggatcccgg
    14041 gtgacgtcga gcatcggcgc gaaggtccaa tggatgccct ccgtgctcgc ctcccgcgcg
    14101 gcgattgcgg ctgcgcgttc gatcaagccg ggattccagg cggcagcctg tccgagaggg
    14161 attgggaaca cggtgcggta tccatgaatc acgtcgcggc caaacaggag cgggattccc
    14221 agccggctct cctggatggc aatccgctgc gcttccgccc gatcttccgg cagcccggca
    14281 ttcaggaaag atccccagcg cccctgccgg atctgctgtt tgatctcttc tgcgatcggg
    14341 ttgcgcatcg aggtcgcctg cgacatctgc ccgagctttt cctcgagcgt catgcgagca
    14401 agaagggcat cgatgcgctt ttcgatgtcc gccggaagac ttgtctggag agccaaagtc
    14461 atggagagtg taagaaagag ttgcccgaac atgccagcgc cccttctgct ttcatcgtac
    14521 ccgccccgga acacccgcgt tgcgggttag cgctcgacgc cgcgcaggcg gcggtgcata
    14581 gcggcaaatt gttcgagcgt gagttgctcc gcgcgcaatg ccgcctccgg ccaggcatcg
    14641 acctgatggc catagccggg aaggaggtta ttccggatcg tcttccgctt ctgccggaag
    14701 cattgtccga cgaatgcgag aagctcttcc gggtgttcga ccggcggcgg atgcggcgtt
    14761 aaccgcacga cggccgattc cactttgggc ggcgggtgaa aggcggcggg cggaaccgta
    14821 aacagatatt cggcacgcgc gtagatcagg atcgcaaccg tcagatagcc gtagtcgcgc
    14881 gatccgggtt cagccaccag ccgctgcgcg acttctttct ggatcaggaa aaccgcacgg
    14941 gtcagcagcg gccccagagc cagcaccttt tcgatcaccg gcgacgtgat gtagtaaggc
    15001 aggttgccgg cgacaacggc cgcgccccat tgcgcaagat cggtggtaag aacgtcgccg
    15061 gagatgacgg taatatcggg aaaccgcgac cggagacgca gcgcgagctc ttcgtccaat
    15121 tcgatcgcga tcacctcgcc ggccttcgag accaggtggg ctgtcagggc gcctttgccg
    15181 gggccgatct cgatgactcg cggcgcaggt tcggggcagg cggcacgcgc aattcgctcc
    15241 agaacggagc cgcggaccag gaaatgttgt ccgagccgcc gccccatagt ctaaacacga
    15301 cgtactgaat gtttgcgcga agatttgatt ggccacggat ggacacagat gaacacagat
    15361 aagaaaaacg caaggcttga tccgtgtgaa tccgtgttca tccgtggccg aatttgcttt
    15421 tctagattgc tgggaatcgt acggcggaga aactcagcct tttacctcga tatggctctt
    15481 gctgaaggag acgcgcactt ttccggccgt cggctgtccg aaggtggtag ctggagtgaa
    15541 tgtcgtaaag agcaggttgt tttcgataat ccgcagcagc tcgggattct cggaaacttt
    15601 cgacgggaac gagtaatcca ccatcctgcc ctgttcatct acagtgagtt caacgataat
    15661 atcgtcatct ccgaacgtga acgggccggt gctcttcacg gttgcttctg tataaaaagc
    15721 cgtgggcaca tcatttcccg tgaagctctg gtaggtggcg acattcggca ccaccatgct
    15781 gaacaggaag actgccgagg cgagtccacc cgcgagtgga atcgccagcg gacgcatgag
    15841 gttatccctc caaagccgcg tgctggcggc ccacttctcc caaatgttgg aaagagtggc
    15901 gctcttgcgc ctccgggttt gctccttcga gccgattacc cggagcgaaa atgtcaaact
    15961 ggccggcggc gtctgcgcgg gcagtgccct cagccggctg cggagaacat cggtctgctc
    16021 gcagcgcaca gtgcatttcg ggcaatgcgt aaggtgcagg gaaatggtct ggctctcggc
    16081 ttgcggcagg aagccatcca ggtacgccga cagattcttc tccacctcat ggcacttcat
    16141 ggctcgcacc ttggtcccag agccggctgc ggtgtccaac tccaggcggg tcccggctcc
    16201 agttgacccg ccaaggtctt acgtaaggcc tcgcgtccgc gtagaattct cgattttacg
    16261 gtgcccagtg aaacctgcag gacatccgcg atttcttcat agctgagccc ctcgatgtcc
    16321 cgcagaacta cagcggcgcg gaacgtcgga ttcaactgcg cgagcgcgcg ttcaatcagg
    16381 gcctgggttt cgtggctgag cgtctcttca aaaggcgagc ggctggaatc gcacagagtc
    16441 tcctggtaat tccggttccg ctcctcgtcg ctctccaaac cgacttcctg gcggcggtgg
    16501 cgtgtgaacc agcgtctctg gttgtgcgcc tcattcaccg caattcgata gatccaggtt
    16561 ttgaggctgc tttgccgccg gaacgagccg atattccgga agaccttcag gaaaacatcc
    16621 tggacgacgt cgcaggtatc cgaggtatcc gtgagcaggc gatgaaccag gttatagaca
    16681 ggctgctgga attgggaaat cagcgcctcg taagcctcat tttcgccggc ttggagcccc
    16741 tccaccaaca gttcttcctc ctgctggtga caatcggcgc agggcgccag atcgtaggcc
    16801 gcgacatgcg agccgaagtg atagccaatg gtcgtatctg ccatggcaag cctcacccgt
    16861 attctacatc agcggaacgg tctacctgcg ccggcccgct ccaacagcat gggcctctca
    16921 aaactgatta gacggctccg gccgctgaaa gttccctcta aacggaatcg gactcttttg
    16981 ttctgattgt tgtggatttg ctgacggcgt ccagtactcc attgacgaac gcgacggagt
    17041 cgtcgcccga aaaccggcgg gccagttcca gcgcttcatc gatggctacc ggcgcgggag
    17101 tgccgagata ccgcatctcg tagacggcgc agcgcaggat attgcggtct acggccggca
    17161 tccgttcaat tcgccagttg gcggaatgcc cggcaatgag cagatcgatg tcggccagtt
    17221 ggctgacggt gccacgaacc agttcctcca tgaatgcatc ctgttcctcg atattcgccg
    17281 gatgctcgtc ggaagaagag agtgtgccgt aaaaggcggc gatggcctcg tccacttcct
    17341 gtttccgcat atccaactga taaagaattt gaacggctct ttcgcgggag gaatggcgcg
    17401 aaggcatgcc ggtctaccgt ccggcgcgaa gctggcgcac gaggttaccc atctcgacgg
    17461 cggtcatggc tgcgtcgaat cccttgttac cgctctttgc gcctgcgcgg tcgatcgcct
    17521 gctcaagcgt gccggtggta aggacaccga aagccaccgg tacgccatgt tggagggaag
    17581 cttgagccag ccctttcgcg gcctccgccg cgatgtaatc gaaatggggc gtttcgcctt
    17641 ttatgatggc gctgaggcag atgatgccgt cgtgtttctt ctgcgcggcg atttcgtttg
    17701 cggccatggg aagctcccag gaaccgggaa ctttcacgat ttcgatatct tcctcggcgc
    17761 acccggtgcg cgccagcgcg tcaagggcgc ccgcgagcag acgctccgta atgatgctgt
    17821 tgaagcggct caccagaatg gcgaacttca gtccttgcgc gctgagattt ccttcaatga
    17881 ttctgtgcgg catgtgctat ttccctgcga aaacagcctt tcttttttcg agaaatgcgc
    17941 gggttccttc ccggcggtcc tgggtgtccg cgatcaggcc gaaggcggcg gcttcgaatc
    18001 gcagcccttc ctccagcccc gcgctggttc ccacatccac agcttccagg atgagcgaga
    18061 tggcgatggg gccgttcgtg agcacctttt cgagcaaccc gcgcgcgaac ggctgaaggt
    18121 tctcacgcgg cgtcacgtag ttcaccaggc cgatgcggtg cgcctcttcc gcgtccacgg
    18181 gttcgccgct cagcagaaga tcgagcgcgc gtccgcggcc caccagacgc ggcagccgct
    18241 gtgtaccgcc atagcccggc agaagaccga gcttcacttc cggttgcccg agtttcgctt
    18301 cgggacatgc tacccgaagc gtgcaggcca tggccaactc gagacccgcc cctagcgcat
    18361 agccgttaat gcaggcgacc gatggctttc ccaacgtctc aagcttacgg aatatctgtt
    18421 ggccccgaag agcggcttgc cgagcctcca ccggcgtcat ttccgacaac tccccgatat
    18481 ccgctcccgc cacgaacgct ttgtcgccgg ccccggtgac gaggagcgcg ccgatggtag
    18541 catcgctccg cgcacgttcg aacgcatcgg cgagttcttc catcacgtgc gcgttgagcg
    18601 cattccgttt ctcgggacgg ttaatcgtga cgagtgcgat gcggtccgcc ccggcctcaa
    18661 accggatgtg gctgtaggac atatgggata atcggactca gagcctcatc ataccgcatg
    18721 gattccgcgt tcattcgaaa cttctctatc attgcgcata tcgatcacgg caagtcgacg
    18781 ctggccgacc gcctgctgga ggtaaccgga gctctctcgc agcgggaaat gatggagcaa
    18841 gtgctcgact ccatggacct ggagcgggag cgaggcatca ccatcaaggc acatgctgtc
    18901 cgcctgaatt accaggcaag cgacggcgag acgtaccagt tgaacctgat cgacacgccg
    18961 ggccacgtcg atttcagcta cgaggtttcg cggagcctgc aagcctgcga gggggcgctg
    19021 ctggtggtgg acgcctcgca gggagtggaa gcgcagacgc tggccaacgc ctacctcgcg
    19081 ctgcaccacg atctggagat tattccggtc atcaacaaga tcgatttgcc ggcggcggac
    19141 cccgagcgta tccgcgcgca gattgagcag gtcgtcggcc tcgacgcctc ggacgcgatc
    19201 ctggtaagcg cgaagcaggg cgttggagtg gaggatgttc tcgaagccgt cgttcacctg
    19261 gtgccacctc caaagggctc accggaaagc ccgctgcgcg cgttggtttt cgattcctgg
    19321 ttcgaccctt atcgcggtgt catcacgctg ctcagggtgg tggatggaac ccttcgtaca
    19381 ggccagaaga tccggctgtg gtcgaacggc aagtccttcg aggtggaggg actcggttac
    19441 cagtcgccga aggccatacc ggccgaacag ctttcggccg gagaagtcgg ctacatgttc
    19501 gcgaacatca agacggtgtc cgacgcgcaa atcggggata cggtcactga cgatgcaaag
    19561 ccggcggctc aaccgcttcc cgggttcgag cagatcaagc cgatggtctt tgccggcctc
    19621 tatccggtgg agtcgcacga gcacggagtg ttgagggaag cactggaaaa gctgcgcttg
    19681 aacgatagcg cgttcagttt cgagccggag agctcggtgg cgctgggctt cggattccgc
    19741 tgtggtttcc tgggcctgct gcatctggaa atcgtacagg agcgcctgga gcgcgaattc
    19801 cagatcgacc ttattacaac cgctccgggt gtccggtacc gggttgtgct gcgggacggg
    19861 gatgtagtgg aagtagacag ccccaccaag tttccggatc cggcgaaaat cgataagatc
    19921 gaagagccga tcattgaagc caccgtgatc acacgcgaag aatttctggg tgagatcctg
    19981 aagttgctgg acgaaaagcg gggcgtacag aaaaaattcg agcatatcgg cagcgacagg
    20041 gtgctgctca cgtatgagat tccgttgaac gaggtcgtgc tcgattttta cgaccggctg
    20101 aagtcggctt cgcgcggcta tgcttcgctc gattatcatt tggccgggta ccgcgaatct
    20161 ccgatggtca agctggacgt gctggttgcc ggtgaaccgg tggacgcatt gtcgatcatc
    20221 gtccacaagg atttcgccca cgaacgcggc aaggccctga ttgaacggct gaggaagttg
    20281 attccacgcc agatgttcga ggtggcgctg caggccgcga tcggcaataa agtggtggcc
    20341 cgcgagaata ttcccgccat tcgaaagaac gtgatcgcga aatgctatgg cggggatatt
    20401 acgcgcaagc gcaagctgct tgaaaagcag aaggaaggca aacgccgcat gaagagagtg
    20461 ggccgtgtgg agattccgca ggaggcgttt ctcgcggtgt taagagttgg tggaaatcaa
    20521 acatagccgg ctcatgccgg acgcaatcaa aggtttcgtg atgagaaaat catttctctg
    20581 tctgcttagt ctcgttctcg ctctggtggc gtcggccgct ccgccgcagg cggctcccca
    20641 gggtaccccc aagttgatcc tgacgctggt ggtcgaccag ttccggtacg attatctgac
    20701 ccgtttccga tccgattaca acggcggatt ggcccgtctg ctcacccatg gcgctgtctt
    20761 ttcaaacgcc cactatgagc attttccgac ggtcacggca attggtcatt ccacgattct
    20821 gagcggagcg acaccctcca tcagcggaat tgtcggaaat gaatggtacg accgtgaatt
    20881 gaacaaacaa gtgaccagcg tttccgacag caccgtgcag ttgctgggag gccgggccgg
    20941 cgaggccggt gcgtctccgc ggaaactgct ggtgagcacc atcggcgacg agctcaagat
    21001 gtcggggaag ggcacgcccc gcgtgatcgg aatgtcgatc aaagaccgca gcgccattct
    21061 tcctgcgggt cacatggccg acgcagccta ctggttcgac gatgtgacgg gcaacttcgt
    21121 cagcagtact tactattccg ccgacctacc ggcatgggtg aaggcattca acagcggtcg
    21181 agcggtggac cggtacataa ccaaacagtg gtcctccaat tccggtacgg cgggcgcggc
    21241 gccgcaggtg ttcgccacga tgccgccgaa gccggacagc gcttatttca agctgctgga
    21301 gcggacgcct ttcggcaatg aggtgttgga gttatttgcc gagcacgcca ttgaagcgga
    21361 gcaactcggc cagcgcggca cgacggacct gctgtcggtg agcttctctt ccaacgacta
    21421 tgtcgggcac ggcgtggggc cggacgcgcc ggaagttcgg gacatctcga ttcaaaccga
    21481 ccggctgttc gccagattgc tggatttcgt cgaccgcaaa gtggggctgg acaacgtgct
    21541 ggtgatcttt acggcggatc acggagtgtc gccgcttccc gaactcatga agcaacgaaa
    21601 gatgccgggc ggcagaatgt ccgcgcgcgt ggtgctggac ggtgtgcaga aggcgattga
    21661 agcgaagtac ggggaaggaa actgggttat cggcaagtcc ggcccggctc cgtatttcaa
    21721 ctaccaactg atccgcgagc gcaagttgga tctggcagag gtgcagcgag tggccgccga
    21781 cgcggtccgg aacattccac acattgcccg ggtctatact cgcgagcagt taaagaacgg
    21841 gcagattctg gaagaccgga tcgggaggcg cgtccgcaat ggcttcaata acgagcgggg
    21901 atccgacctt tttattgtcg tcgagccgta ctggctgttc gaggaacggg gcacgtccca
    21961 cggcacgccg tataattacg attcccacgt gccagtgatt ttcatgggcc gcgggatcaa
    22021 ggcaggccaa tacaaccagc ccatcgcggt aaacgacatc gcgccgaccg tcgccacgtt
    22081 tttagagatc gaaaccccca gcggcgcgga aggccgcgtc ctggatgaaa tgctgacagc
    22141 gcccgcaccg ggcgtacacc tgtcatccta tgtggagaaa tagactcgag gagtgattta
    22201 tggacattcg accactgcat gatcacgtgt tagtgaaacg catcgaggaa gctgagaccg
    22261 tgagcggcag cggtatcatc attccggaca ccgccaaaga gaagccgcag caaggcgaag
    22321 tgatcgccgt cggcaatggc aagctgctca gcaatggcga gcgcagcgtg atcgacgtca
    22381 agccgggcat gcggatcctg tttacgaaat atgccggctc ggacattaaa agcgcgggtg
    22441 aggaatacct cgttctgagg gaagacgaga tcctcggagt actggagcag taggtacccc
    22501 ggttggtctt ccggtctgtg ctaaggtgaa aacaaagaac taacgacccg tgaggaaggc
    22561 ggtgggccgc tcgtggcggt attttggccg cggtgtgtag cgagcggata ccgcttgctg
    22621 acgcgcgcgg ctccgttctg agccgtgacc gtaagggagc ggtgccgttg aaaaaggagt
    22681 cttgaagaag aatgacgaga gcctcgttcc gtctgatagt aatgggagcc gcactgctgg
    22741 catcggccag tctggcgggt gcgcagacaa aagtagcggt ggtgaatctc cagcgtgccg
    22801 tgttggaaac ggctgacatc aagaaggcac aggcggacct ggaagcccgg taccggccgc
    22861 agcagcagga aatggagaag ttgcagaagg aagtcgaggc tctatcgcag caacttcaga
    22921 cgatgcgggg taagttgacg cagcagggcg agcaggatct tgttgtgcaa ggacagcgta
    22981 aacagcggga gttgcagcgc ctgacggaag accttcaggg agaagtgaac cgcgaccgcg
    23041 aagagattct tgccagggtg tctcaacaga tgtcggaagt ggtgggcaag ctcgccacgc
    23101 agaaagacct cgatatcgtc gtcgacgtga caaataccgt ttttttcaag cccgcgctcg
    23161 atctgaccaa ggaagcggtg gcggcctacg acgcggccca tcccgtaaag tgaggtgagg
    23221 gaatgtccgg ctacctggaa gcatacgggg ctggcgacga gaagcgttcg aagattatca
    23281 gatggaccgc catcacggtg gtggcggtgc ttgttattgg tctcttcctt tatttcgggt
    23341 tccggaatta ccgcgaagac cggatagcca atcagttcgt cgaattcctc aaaacgcagg
    23401 actatcaggc ggcataccgg ctgtggggct gtacgcctga aagtccatgt cccgactact
    23461 cgattgaaaa gttcatggag gactggggtc ccaaaagtcc ttacggctct ccggcgaatg
    23521 cggaggt
//
LOCUS       DASTMK010000373        15194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_370177, whole genome shotgun sequence.
ACCESSION   DASTMK010000373 DASTMK010000000
VERSION     DASTMK010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15194
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370177"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..2509)
                     /locus_tag="VFQ79_16295"
     CDS             complement(86..2509)
                     /locus_tag="VFQ79_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0141284.1"
                     /translation="MPIRLIALFAVIGLAAVLSSRPAPREQAGPIDGGYLLVSGWKSR
                     PAGKQVPLDTFPMSCALSKDGKHLLVLNAGYKPPSISVLDVASMKETSRVPVKDGWLG
                     LTFHPGGDRVYAGGGSSGSVFEFSFEDGKLEPARVLQLSSEARAFIGDVAIAPGGNVL
                     YATNLYHDSVVRVDLASGSAPSHIETGRRPYRIIFHPEGESFFVSNWADNTVVEHEAA
                     TGRVRSRLDAGAHPSAMAWKDGRLFVAAANTNKVYVLSREGEEWKPSEQINISLTPRQ
                     PVGMTPSALAFNHDLTRLFVVCSDGNSVAVVDTSKPAARVLGFLPAGWYPTEATVLPD
                     GTLVVLNGRGQRSYPNPLGPSPVRRAVPQSQGTGEVEYVGRIQIGSASFIPPFGDREL
                     ASYTKAVLENSPYRDSKLDDAGTGRNNPIPSRPGGKTPIEHVVYIIKENRTYDQVLGA
                     LGKGNGDPSLVLFGEDITPNQHKLAREFVLLDNFYVSADVSADGHNWSMAAIAPDFTQ
                     RVWPNGYGRRGTHRYLFGQEPAAATPNGYLWTHVHEKGLSIRNYGYLGVHKNPAPADG
                     VQIADVLDSILKPVTCLEYRAYELDYPDIGRARAFLRDLAQHEEKGEWARFVLVRLGN
                     NHTQGTTPGRLTPASHVADNDLALGMIVEGITKSRFWPKTAIFVLEDDAQNGPDHVDS
                     HRSPAFVISPYVRRGAIDSTMYNTTSMLRTMELLLGVGPMTHFDAGAHPMSAAFQRKP
                     DFKPYQAVPARVPLNDRNPANSPTAARSLALDFSEADLIDDDELNEILWLAMRGTQPP
                     APVRSYFSR"
     gene            complement(2515..4998)
                     /locus_tag="VFQ79_16300"
     CDS             complement(2515..4998)
                     /locus_tag="VFQ79_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HEU0141285.1"
                     /translation="MKFILPAMLLVLAAILTSQPAPREQPGPLPDGGFLLNSGWKIKP
                     AGRQVPLDTFPMSTALSPDGAFLLVLNGGYKPPSISVLDAASMKEIGRTPVPDGWLGL
                     AFEPKGKQVYVGGGSRASVFEYSISPEGRLEPARTFPIVPEKDRSHRDFIGDVALSPD
                     GRLIYAAGLYQDTIFVINPQSGMVIERFKTGRRPYRILFHPDGKSFFVSSWVDGTLLH
                     HNAQSGEILARVRLGPHPTDIVWSSRKAPVEEGQAAPAWTARMFVAAANTNRVYVVDI
                     NESRNLRVIESINVAMTPNQPLGMTPSALALNADESRLYVVCSDANAVAVADVTGQRS
                     RVLGFVPTGWYPTAARAMKDGTLVILNGRGLRSYPNPRGPNPAQRPEPVHKGTAAPEY
                     VGRLQTGSASIVPPFNDERLEEYTATVLANSPYEDVKLIGVNVPADSPIPPSPGAPSL
                     IQHVIYIVKENRTYDQVLGGLEKGNGDPSLVLFDEKASPNHHKLARDFVLFDNFYVNS
                     DVSADGHNWSTAAIAPDYVQKLWPNSYGGRRRHYDYEGTEPAATPPAGYLWTNAAAAG
                     VSMRNYGYFVNNLPKPGPDGIQVGSVRDPVLDKVTNRKYRGFDMDYPDIERAKVLLED
                     LAQFEQSGEMPRLLLVRLGNDHTSGTAPGKIAPLSAMADNDYALGMIVEAVSKSRFWP
                     TTAIFVLEDDAQNGPDHVDSHRSVAFVLSPYARRGAVDSTMYNTTSVLRTMELILGLR
                     PMTHFDAGARVMTACFQAKPNLKPYTAEKPRMPLDDRNPASSPTAARSARLDLSEADR
                     IDDDELNDILWRAIRGTEPPAPVRSFFAR"
     gene            complement(4995..6644)
                     /locus_tag="VFQ79_16305"
     CDS             complement(4995..6644)
                     /locus_tag="VFQ79_16305"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU0141286.1"
                     /translation="MRIALGQIDTTVGDLAGNVDRMVRFAKDAAGHGAELIVFPELSV
                     TGYPPRDLLEKDAFIEKNLLELERLAAETASLDIAIISGYAGRANGVPGKQITNSAAI
                     IEKGRVIFHQDKMLLPTYDVFDEARYFYAAERQAAVNLRGRKIALTICEDAWNDKQYW
                     ERRLYQRDPVDELVQSGAELLITINASPYHMGKRKLRREIFAATARHHRVPVVYVNQV
                     GGNDQLVFDGSSFVMSREAEVIASAASFREDLVLLDLDTGSGDRNENLHNECEAVYEA
                     LVLGTRDYIQKCGFRRVLLGLSGGIDSSLVAVIAADAVGRENVFGVTMPGPYSSAHSV
                     ADSRALAERLGIGFEVLPIDGVYQSYLAALDPLFRGAARDVAEENIQARIRGAMLMAL
                     SNKWGALVLTTGNKSELAVGYCTLYGDMCGGLAVISDVPKTLVYELSRIANQRHPEAI
                     PENVFVKPPSAELRPNQKDTDSLPEYAVLDDILKGYVEDAKSAAQISEELGLPRELVR
                     DIINKVDRNEYKRHQAAPGLKVTSKAFGIGRRFPIAQRFLE"
     gene            complement(6737..9370)
                     /locus_tag="VFQ79_16310"
     CDS             complement(6737..9370)
                     /locus_tag="VFQ79_16310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141287.1"
                     /translation="MPWRWLYTVPLRLRSLFRRAQVESDLEEELRFHLEQQIEDEIAR
                     GKSPQEARYTALRRMGGLEQRKEECRDTRHMNLIDNLMWDVRYAMRMLARSPGFTVVA
                     LLALALGIGANTAMYSIVHAVMFRPLEVRDPDRLVRVYESNRSRNLPAFSASVRNYLS
                     WKEQAHSLDLAVFQGYAANWTQDGEPERLEGMAVSASFLPVLGMTARIGRWFHDEEER
                     AGRHRVVVLSERLWAARFGRDPGVLGRKLFLNGEPYSVVGIASGGLTIPVAPDLWVPL
                     VVDPDASRGNRQYTVIGRLRPGFTVQQAQAEMGSIAGSLERQFPESNNGWSVSIVPLM
                     RWLVPAEIRTALLVLLGAVGMVLLIACANVANLLVARAEARRKELAIRAAMGAGASRI
                     SQQLLTESLLLSILGGAVGVAIGYSIVGVARRSLNELVPRANEISIDLTVLGFALSVS
                     VITGLLFGLTPILQLGKMRNLDALHEAGRMSQPAARSRLRAMLVIAQLSLATLLLIGA
                     GLLLQSFARLQNVSLGLDPGSVLTARISLPRARYADGEAVSAFLSRLTEELKSAPGVQ
                     AAGVSNAIPLGPGSTIAGTAVVVGAPDSALGQPVSLGWRSVDAGYFAALRIPLLRGRI
                     FGWEDDTARRRVFVLSQQAALSLYGNSDPVGRQLRLNDAAGEVIGVVGDVRMKSLADP
                     PERVVYLPISQGGRFAVFALFVKISSGSSAAAASLIRERLREIDSALPAYGFRTMSDW
                     VGMSSARTRIRTWTLALLAAIALALGMIGIYGVLAYLVILRRHEFGVRLALGAPPGSL
                     LRLVLGQGLGLAAIGIAIGLAGAVLLTRLLETLLFGVGTHDPMTFLGVAILLLAASLI
                     ACYAPARRAAGADPIAALRTE"
     gene            complement(9377..9712)
                     /locus_tag="VFQ79_16315"
     CDS             complement(9377..9712)
                     /locus_tag="VFQ79_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141288.1"
                     /translation="MSKPSDLVQGTLDLLILKILALEPLHGWAISQRLKQVSGDVLQV
                     SDGSLYPALHKLEQEGWITAEWKTSELGRRAKFYSLTRIGRRHLEKEAANWERLSAAI
                     SQVVRLKEA"
     gene            9796..10524
                     /locus_tag="VFQ79_16320"
     CDS             9796..10524
                     /locus_tag="VFQ79_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU0141289.1"
                     /translation="MSAFHGWAGSPDVRGLPFAVLISADAEWQVVKTLFATVVIKTSP
                     YGEYFFANIDRDTVLFFQGGWGKVAAAGSTQYVIDHFHPVRLINIGTCGGVEGRIKCF
                     DVVVIEKAVIYDIEEAIGDSKQAIAAYSTSLSLPPRFPVPVVKVTMYSADRDLTAAGF
                     RELDTQHQLVVADWESGAIAWVAHRNATPVLILRGVTDLVNADSSEAQGNLQLFQNNT
                     SRVIHNLVGDLPKWLAEFRIGVLS"
     gene            10535..10951
                     /locus_tag="VFQ79_16325"
     CDS             10535..10951
                     /locus_tag="VFQ79_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141290.1"
                     /translation="MNRDDIGLLPAISFGTLILFYFVWAAMHDIAHGDEGTLEWTVLA
                     ICAVAFAGLYRQALWFLTPKAKVAWLIGTGLLLVLYSAGAISSMLHPKYVKDPMLART
                     FLMVGSPALALISYHLVREALHSRARRGWHHAHTNS"
     gene            complement(10974..12017)
                     /locus_tag="VFQ79_16330"
     CDS             complement(10974..12017)
                     /locus_tag="VFQ79_16330"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0141291.1"
                     /translation="MKHTAGKPSNGDAVRPGQIGAGVIGLGLMGHSIIACLLAAGHRV
                     TAITRSTAKHRGTPRHILALLQEMKREGLLRKDPVSLMPKFAISEDFADLAGCGIVIE
                     SIIEDIDTKKQTYRVVEENVAGDTIIGSNTSSIPLTLLQQGAVRPERFVGIHWDEPAH
                     VTRFMEIIAGEKTERKHTDKVMALARAWGKEPSLVRRDVRGFITNRVSYAMFREACHL
                     VDSGIATVEDVDRSLRNDVGWWITFAGPFRYMDLMGVEAYARVMKDLLPELSTSPDVP
                     PLMRGVIEAGGRGISNGRGFYKYTPEQAKRWEKLFLKFNYEIRRLAMRYPEDIGNRTG
                     RKKPARKAARSGA"
     gene            12163..14631
                     /locus_tag="VFQ79_16335"
     CDS             12163..14631
                     /locus_tag="VFQ79_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEU0141292.1"
                     /translation="MTTRLTEAERRTAGSLSAYGKARSTVQGAPLTEEDLSRIDAYWR
                     ACNYLALGMIYLQDNPLLREPLKPEHIKNRLLGHWGASPGLAFIYVHLNRLIRKYNLN
                     MIFLAGPGHGAPGVLGPVYLEGTYSEIYPEKSQDEDGLREFFKQFSFPGGIGSHCTPE
                     TPGSIHEGGELGYVLSHACGAAFDNPDLIAAAVVGDGEAETGPLATSWHITKFLNPIR
                     DGAVLPILHLNGYKINNPTLLARISHEELEQLLRGYGWTPYFVEGSDVATMHQAMAAT
                     VEHCVLDIRKSQDECRKTGTPARPRWPMIVLRTPKGWSAPAEVDGHRLEGFWRAHQVP
                     LAGVKSNPEHLRVLENWMRSMKPEELFDETGRLIMDLRDLAPAGNRRMSANPHANGGL
                     VKRSLRLPGFRDYAVKVERPAAMEVENVPPLGRFLADVMKRNPDNFRVFAPDENTSNK
                     LDAIYEATKKFWIAEYFPEDQDGGELSPDGRVIEMLSEHTIEGMLEGYLLTGRNGFLS
                     SYEAFTHVIDSMFNQHAKWLAISNELSWREEIASLNLLITSTVWRQDHNGFTHQDPGF
                     LDIVLNKSAAVTRIYLPPDANCLLSVADHCLRTENYVNVIVSDKQRHLQYLDIEFAIM
                     HCTKGIGIWDWASTDRGTEPDVVMAAAGDIPTQEALAATALLREQFPDVKIRFVNVVD
                     LFKLQPATEHPHGLTDRDFDSLFTVDKPIIFNFHGYPWSIHRLAYRRTNHRNLHVRGY
                     KEKGNINTPLELAIANEVDRFSLAMDVIDRVPALQRIGAHAKEEFRNRQIACKNYAYE
                     HGIDPPEITEWKWPYGPHGEVQNS"
     gene            complement(14619..>15194)
                     /locus_tag="VFQ79_16340"
     CDS             complement(14619..>15194)
                     /locus_tag="VFQ79_16340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141293.1"
                     /translation="IFYTDLRHAFTLVEDEVPGQTFRRLLRVNGPGAHVAGVDFDGTI
                     QLNSRLGLRGGFTVQQARWKEPEEQFGASDFFRAPKRYGFLGLDWDLPHEIEVTGTAD
                     FTGSMLVPHYAGYINEDRLETSKRFGVFNAVVSKTFTVTDASKIRLFFNMQNIGDSYQ
                     KDLDQGPSRDSAYVYGPAEMRRAVVGLTYEF"
BASE COUNT         3195 a         4558 c         4420 g         3021 t
ORIGIN      
        1 tcgcggctca gaaacaaagg ccttacggag ccgcgcgcat cagcaagcgg tcgtgacttt
       61 tgaagcaaac ttccgtgaca gaacattagc gtgagaagta actgcgcacc ggagcgggag
      121 gctgggtgcc gcgcattgcg agccagagta tctcgttcaa ttcgtcgtcg tcgatcaggt
      181 ccgcctcgct gaaatcgagg gcgagcgacc gtgcggcggt aggcgagttg gccgggttcc
      241 ggtcgttcaa aggaacacgg gcgggcacgg cctggtacgg cttgaagtcc ggttttcttt
      301 gaaacgccgc cgacatcgga tgggcgccgg cgtcgaaatg cgtcatcggc cccacgccca
      361 gaaggagttc catcgtcctc aacatcgagg ttgtgttgta catcgtgctg tcgatggcgc
      421 cacgccggac ataaggagaa attacgaatg caggcgagcg gtgggaatcc acgtggtcgg
      481 gaccattctg cgcatcgtct tccagaacga agatagcggt cttcggccag aagcggctct
      541 tcgtaatgcc ttccacgatc attccaagag ccaggtcatt atcggccaca tgcgacgcgg
      601 gagtcagcct tcccggcgtg gtgccctggg tgtgattatt ccccaaccgg accagcacga
      661 agcgggccca ttcgcccttc tcctcatgtt gagcgaggtc ccgcagaaaa gcccgcgcgc
      721 ggccaatatc cgggtaatcc agctcatacg cgcgatactc caggcacgtc accggcttca
      781 gaatggaatc cagcacgtct gcaatttgta ctccgtccgc cggtgccgga ttcttgtgaa
      841 ccccgagata accgtagttc cgtatggaca atcccttttc gtgaacgtgc gtccagagat
      901 agccgtttgg tgtcgccgcg gcgggctcct gaccgaaaag gtaacggtgc gttccgcgcc
      961 tgccgtatcc gttgggccaa acccgctgcg tgaaatccgg cgcgatggcg gccatggacc
     1021 agttgtgccc gtcggcgctc acatcggcgc ttacataaaa gttgtccagc agaacaaatt
     1081 cgcgagccag tttgtgctgg ttgggagtga tgtcttcgcc aaacaatacc agcgacgggt
     1141 cgccgttgcc tttgcccaac gctcccagca cttggtcgta cgtgcgattt tccttgataa
     1201 tgtacaccac gtgctcgatg ggcgttttac cgcccgggcg cgatggaatg gggttattgc
     1261 ggccggtgcc ggcatcgtcc aatttcgaat cgcggtatgg agagttctcc agcaccgctt
     1321 tggtgtaaga ggcgagctcc cggtcgccaa atggcgggat gaaggacgcg ctcccgatct
     1381 ggatgcggcc cacgtactct acttcgcccg taccctgaga ttgcgggaca gcacgccgga
     1441 ccgggctggg cccgagcggg ttcggatagg agcgttgtcc gcggccgttc aataccacca
     1501 gagtcccatc gggcaaaacg gtagcctcgg tgggatacca gcccgcggga aggaacccca
     1561 acacacgcgc cgccggtttc gacgtgtcaa ccaccgcgac ggaatttcca tcggagcaaa
     1621 caacgaacag ccgggtgagg tcgtggttga atgccagcgc gctcggcgtc atacccaccg
     1681 gctggcgggg cgtcagggaa atattgatct gctcggaggg tttccattcc tctccctcgc
     1741 gcgagagtac gtacactttg ttcgtgttcg ccgccgccac gaaaagcctg ccatctttcc
     1801 acgccatggc ggaaggatgg gcgcccgcat ccagacgcga ccgcacacgg cccgtcgcag
     1861 cctcgtgctc gacgaccgtg ttgtcagccc aattggagac gaaaaaggac tcgccctcgg
     1921 gatggaagat aatgcgatac ggccgcctcc ccgtctcgat gtgcgacggc gccgaaccgg
     1981 aagcgaggtc cacgcgcaca acggaatcgt gatacaggtt cgtcgcgtac aggacattcc
     2041 caccgggcgc tatcgccaca tctccgatga aagcgcgggc ttcggaagag agttgcagaa
     2101 cgcgtgccgg ttcgagtttg ccgtcttcaa atgagaactc gaaaacggag ccgctggagc
     2161 ctccgcccgc gtaaacgcga tcgccgccgg gatgaaacgt caggcccaac cagccatcct
     2221 tgacgggaac gcggctcgtc tccttcatgg aggctacatc cagcacgcta atggacggtg
     2281 gcttgtatcc cgcgttcaac accagcaggt gcttgccatc cttcgacagc gcgcacgaca
     2341 tgggaaacgt gtcgagcgga acctgttttc cggcgggccg cgatttccac ccgctgacga
     2401 gcagatagcc gccgtcaatg ggaccggcct gttcccgtgg ggccggccgc gagctcaaca
     2461 ccgccgcgag acctataacc gcgaagaggg caatcaatcg tataggcatg gatgtcacct
     2521 ggcgaaaaag ctgcgaacgg gcgccggcgg ttcagtaccg cgaatcgccc gccataatat
     2581 atcgttcagc tcgtcatcgt cgatgcgatc ggcttcgctc aagtcaaggc gtgccgaacg
     2641 agcggcggtt ggggatgacg cagggttccg gtcatcgagc ggcatgcgcg gcttttccgc
     2701 tgtgtacggc ttcaaattgg gcttggcttg aaaacaagcc gtcatcacac gcgcaccggc
     2761 atcgaaatgg gtcatcggcc gcagcccgag aatcaattcc atggttcgca acaccgaggt
     2821 cgtattgtac atcgtgctgt ccaccgcgcc gcgccgggcg tacggagaga ggacgaatgc
     2881 aaccgaacgg tgcgaatcca catggtccgg tccgttctgc gcatcgtctt ccagaacaaa
     2941 gatggccgtc gttggccaga agcggctctt cgatacagcc tcaacgatca ttcccagcgc
     3001 atagtcgtta tccgccatcg ccgagagtgg agcgattttg cctggcgcgg tgccggaggt
     3061 atgatcgttg cccagcctga ccagcagcaa ccgcggcatt tcaccggatt gttcaaactg
     3121 cgcgagatcc tccagaagaa ctttcgcgcg ctcgatatcg gggtaatcca tatcaaagcc
     3181 gcgatactta cggttcgtaa ctttgtcgag aacgggatcg cgaacggaac ccacctgtat
     3241 gccgtcaggc ccgggcttgg gcagattatt cacaaagtag ccatagttgc gcatcgacac
     3301 gcccgccgcg gcggcgttgg tccacaggta gcccgccgga ggagtggctg cgggttcggt
     3361 tccttcgtaa tcgtagtggc ggcgccgccc tccatagctg ttgggccaca gcttttgcac
     3421 gtagtcggga gcgatcgccg ccgtcgacca gttgtgcccg tccgcgctaa cgtcggaatt
     3481 gacgtagaaa ttgtcgaaca gcacgaaatc ccgtgccagc ttgtgatggt ttgggcttgc
     3541 cttttcgtca aaaagcacaa gggacggatc accgttcccc ttctccaggc cgcccagcac
     3601 ttggtcgtac gtgcggttct ccttgacgat atagatgacg tgctgaatga gcgacggagc
     3661 gccgggcgaa ggcggaatgg ggctatcggc cggcacattc acaccgatca gtttcacgtc
     3721 ttcatatgga gagttggcga gaaccgtggc tgtatactcc tcgagccgtt cgtcgttaaa
     3781 cggcggaaca atggacgcgg aaccggtctg caagcgtccc acgtattccg gggcggctgt
     3841 tcccttgtgt acaggctccg gccgctgcgc gggatttggt ccccgcggat tcgggtacga
     3901 ccgcaagccg cgtccgttca ggatcacaag cgtgccgtct ttcatcgcac gcgccgccgt
     3961 cggataccag ccggtgggga caaaccccag cacccggctt cgttggccgg tgacgtccgc
     4021 caccgcaacc gcattcgcgt cggaacagac aacgtacagc cgcgattcgt ccgcgttcag
     4081 cgcgagtgcg ctgggtgtca tgccgagcgg ctggttgggt gtcatcgcaa cgttgatgga
     4141 ttcgataacc cgcagatttc tgctctcgtt gatgtcgacg acatagacac ggttcgtatt
     4201 ggccgcggca acgaacatgc gcgcggtcca ggcgggcgct gcctggcctt cctcgaccgg
     4261 cgccttgcgg ctgctccaaa cgatgtccgt agggtgtggt cccaaccgga cgcgtgccag
     4321 aatttctcca ctctgggcat tgtggtgcag cagcgttcca tccacccagc tcgaaacaaa
     4381 gaatgatttc ccgtcaggat ggaaaagaat gcgatagggg cggcggccgg ttttgaagcg
     4441 ctcgatgacc attccggatt gcgggttgat cacaaagatc gtgtcctggt agagcccggc
     4501 ggcatagatc aggcggccat ccggagacag tgccacatcg ccaatgaaat cgcggtgcga
     4561 cctatccttt tccggcacaa tcgggaacgt gcgggcgggc tccagccgcc cctcaggcga
     4621 gatggagtac tcgaacacgc tggctcgcga tccgccgcct acataaacct gttttccttt
     4681 cggctcgaac gcaaggccaa gccagccatc cggtaccgga gttctcccga tctctttcat
     4741 agacgccgca tcgagaaccg aaatcgaagg aggcttatag cccccattca atacgagtag
     4801 aaacgcgccg tccggcgaga gtgccgtgga cattggaaag gtgtccagcg gtacctgacg
     4861 gcccgcgggc ttgattttcc aaccgctgtt gagcagaaag cctccgtccg gcaaggggcc
     4921 gggctgttcg cgcggagcag gctgcgacgt caggatggct gccagaacca gcagcatggc
     4981 gggcagaata aacttcattc caaaaacctc tgagcgatgg ggaaacggcg gccaatacca
     5041 aatgccttgc ttgtcacttt caagccgggc gcagcctggt ggcgcttgta ttcgtttcga
     5101 tctactttgt tgatgatgtc gcggaccagt tcccgtggga gtccaagctc ttcggaaatc
     5161 tgcgcggcgg acttagcgtc ttcgacatag cctttcagaa tatcgtcaag caccgcgtat
     5221 tcgggaagac tgtccgtatc cttctgattg gggcgaagct cggcggaagg cggcttgaca
     5281 aaaacgtttt caggaatggc ttccgggtgc cgctgattgg cgatgcggga caactcgtac
     5341 accagtgttt tcggcacatc gctgatgacc gcaaggcccc cacacatatc gccgtagagt
     5401 gtgcaatagc cgacggcgag ttcgctttta ttcccggttg tgagcaccag cgcgccccat
     5461 ttgttggata gggccatcaa catggcgccg cggattctcg cctggatatt ctcttccgcc
     5521 acgtcgcgag ccgctccccg gaaaagcgga tcgagagctg cgagatagct ctggtagacc
     5581 ccatcgatcg gcagaacctc gaaaccgatg cccaaccgct ccgctaaagc acgggagtcc
     5641 gcaacgctgt gtgcggaaga gtagggtccg ggcatggtga caccgaagac attttcgcga
     5701 cccaccgcat ccgcggcgat gacggcgaca agcgaagaat cgatgccgcc gctcaagccc
     5761 agcagcacac gccggaatcc acacttttga atgtagtcgc gcgttccgag caccagggcc
     5821 tcatataccg cttcacattc gttgtgcagg ttctcgtttc ggtctcccga tccggtatcc
     5881 agatcgagca acaccaggtc ttcccggaac gaagcggcgg aagcgatgac ttcagcttcg
     5941 cggctcataa cgaaactgct tccatcgaag acaagctggt cattaccgcc aacctgattg
     6001 acatagacga ccggcacgcg atgatggcgc gcggtcgcgg cgaagatctc ccggcgtaac
     6061 tttcgtttgc ccatgtggta aggcgaagcg ttgatagtga tcaacaactc cgcgccggat
     6121 tgcaccagct catccacggg gtcgcgctgg taaaggcgcc gttcccaata ctgcttgtcg
     6181 ttccacgcgt cctcgcagat cgtaagcgct attttgcggc cccgcaggtt aacagctgcc
     6241 tgccgttcgg ccgcatagaa gtaccgggct tcatcgaaga catcgtaggt gggaaggagc
     6301 atcttgtcct gatggaaaat gacgcggccc ttctcgatga tggcggcgct attagtgatc
     6361 tgttttcccg gcacgccgtt ggcccgcccg gcataaccgg aaataatcgc tatgtcgagc
     6421 gacgcggtct cggcagccag ccgctccagt tcgagcaggt ttttttcgat aaatgcgtcc
     6481 ttttccaaca ggtcgcgggg aggatatccg gtgacagaaa gttcggggaa aacgatcaac
     6541 tcggcgccgt ggccggctgc gtctttggcg aaacggacca tccgatccac attgcccgcc
     6601 aaatcgccca cagtcgtgtc gatctgcccc agcgcaattc gcatggaatt cactattgta
     6661 ctaagcgagt cttacgttcc gatgaccggc tgcggcagtg tgcaggctcc tgatttcgcc
     6721 ggcgcgccga gcccggctat tccgtcctca atgcggcaat cggatcagcg cccgccgcgc
     6781 gccgggcggg cgcatagcac gcaatcaacg acgcggcgag gagtagaatc gctactccca
     6841 ggaacgtcat gggatcatgc gtgccgacac caaaaagcag ggtttcgagg agccgtgtca
     6901 acagaaccgc tccggcgaga ccaatcgcga tgccgatcgc cgccaacccg agtccttgcc
     6961 ccaacaccag cctgagcaga ctaccgggcg gggcacccag cgccagccgt acaccgaatt
     7021 catgccggcg aaggataacg agataagcaa gcacaccgta gatgccaatc atcccgagag
     7081 ctagcgcgat agcggcgaga agagcgaggg tccaggttct gatccgtgtg cgagcggaac
     7141 tcatgccgac ccaatcgctc atcgtgcgaa agccgtaagc cggcaatgcg gagtcaatct
     7201 cccggagtct ttccctgatc agggaagccg ccgcggcaga cgatccacta ctgatcttca
     7261 caaacagggc aaaaaccgcg aagcggccgc cttgcgatat gggaaggtag acgacacgtt
     7321 cgggaggatc ggccaggctc ttcatgcgca cgtcaccgac cacgccgatg acctctccgg
     7381 cagcgtcatt cagacgaagt tgccggccaa ccggatcgct gttgccatac agactcaatg
     7441 cagcctgctg gctaagtacg aagacgcggc gtctggccgt gtcatcctcc cagccgaaaa
     7501 ttcggccgcg gagaagaggg atgcggagtg ccgcaaagta gcccgcatcc accgagcgcc
     7561 acccaagact cactggctgc cccagagcgg aatccggcgc gccaaccact acggcagtgc
     7621 ccgcaatggt cgagccaggt cccaggggga tggcgttaga aacgcccgca gcctgaacac
     7681 cgggtgccga tttgagctcc tccgtcagcc gcgagaggaa tgccgaaacc gcttccccgt
     7741 ccgcatatct tgctcgtgga agagatatcc gtgccgtaag aacggagccg ggatcgagtc
     7801 ccagcgagac gttctgtaga cgtgcaaagc tttgcaggag cagcccagcg ccgatgagaa
     7861 gcagagttgc caaagaaagc tgagctatca cgagcatcgc gcgcaatcgg ctgcgagctg
     7921 ccggttgtga cattcggccc gcctcatgaa gcgcatcgag attccgcatc ttgccaagct
     7981 gtaggatcgg tgtcaatcca aagagtagcc ccgtgatcac agacacactc agcgcaaaac
     8041 caagtacggt caggtcaatc gatatctcgt tggcacgagg aacaagctcg ttgagcgagc
     8101 ggcgcgcaac acccacaatg ctgtatccaa tggcaacacc aaccgcccct ccaaggatcg
     8161 acagcagcaa gctttctgtg aggagttgtt gagagattcg cgatgcccct gcccccattg
     8221 ctgcgcgaat ggcaagttct ttgcgccggg cttcggcgcg ggcaaccagc agattggcga
     8281 cattggcgca ggcgataagt aacaccatcc ccactgctcc cagcagcacg aggagcgcgg
     8341 tgcgtatctc ggccgggacc agccatcgca tcagaggtac gatggagaca ctccagccgt
     8401 tgttcgattc gggaaactga cgctccaggc tgccggcgat cgaacccatt tcggcctgcg
     8461 cttgctgaac cgtgaatcct ggtcttaaac gtccgattac cgtgtattgg cggtttccgc
     8521 ggctcgcgtc tggatcgact accaacggaa cccagagatc gggagcgacc ggaatggtaa
     8581 ggcccccgga agcgattcct acgacactat agggttctcc attcaggaac agcttacgac
     8641 ccagtacacc gggatctcgt ccgaatcgcg ccgcccacaa tcgttcgctc aatacgacga
     8701 ctcgatgccg gccggcgcgt tcttcttcat catggaacca gcgcccgatc cgtgcggtca
     8761 tgccgagcac aggaagaaac gaggcgctaa cggccatgcc ctccagccgc tcgggttcgc
     8821 catcttgggt ccaattggct gcgtatccct ggaagacggc caaatccagg ctgtgagctt
     8881 gctccttcca ggaaagataa ttgcgcactg aagcggaaaa agccggcaaa ttacggcttc
     8941 gattggactc gtaaacccga acgagccgat ccggatcgcg cacttccagc gggcggaaca
     9001 tgactgcatg cacgatgctg tacatcgcag tgttcgcacc gatgccaaga gcgagagcaa
     9061 gcagcgccac tacggtgaat ccaggactgc gggccagcat gcgcatcgca taacggacat
     9121 cccacatcag gttgtcaatc agattcatgt gacgcgtatc acggcactcc tctttgcgct
     9181 gctccagccc ccccatcctg cgcaaagcag tgtatcgggc ctcctgagga gacttgcctc
     9241 ttgcgatttc atcctcgatc tgctgctcca gatggaagcg cagttcttcc tccaagtcac
     9301 tttccacttg cgcccgtcga aagagcgagc gtaaccggag cggaaccgta tagagccatc
     9361 gccagggcat gccgcctcac gcctctttca accggaccac ctgcgagatc gccgcagaga
     9421 gccgttccca attcgcggct tctttttcca gatgccggcg gccgatccgc gtcagagagt
     9481 agaatttagc tcgccggcca agctcgcttg ttttccattc cgcggttatc cagccctcct
     9541 gttccagttt gtgcagcgcc gggtagagcg atccgtcgct gacctgaaga acgtcgccgg
     9601 agacctgctt caaccgctgg ctgatggccc acccgtgcag cggctctaaa gcgagaatct
     9661 tcaggatcag gagatccagc gtcccttgaa ccagatcgga aggtttgctc aaagcgtttg
     9721 tcctcggtta ccgagatcat agctgaatga agaccgctac gcaagagtta cggtgtctcc
     9781 cggtagaatc tgcctatgag cgcctttcac ggatgggccg gttccccgga tgtcaggggt
     9841 cttccattcg ccgtgttgat ctcggcggac gccgaatggc aggttgtgaa aacgctcttc
     9901 gcaacagtgg tgatcaaaac ttctccttat ggagaatact ttttcgccaa catcgatcgg
     9961 gataccgtcc tcttttttca aggagggtgg ggtaaggtag cagccgctgg atccactcag
    10021 tacgtaatcg atcatttcca tcccgtacgg ctgattaaca tcggaacctg tggcggcgtg
    10081 gaaggccgca ttaaatgctt tgatgtcgtc gtaattgaga aagcggtaat ttacgacatc
    10141 gaggaagcga tcggcgactc gaaacaggca attgcggcgt actccacatc gctttccttg
    10201 ccacctcgat tcccggttcc ggttgtcaag gtcacaatgt actcggcgga tcgggacttg
    10261 acggctgccg gtttccggga actcgacact caacaccagt tggtcgtagc cgattgggaa
    10321 tcgggggcca tagcgtgggt cgcccacagg aatgcgacgc ctgtactaat cctgcgtggg
    10381 gtcaccgatc tggtgaatgc ggatagctcg gaagcgcaag gcaatttgca gttattccag
    10441 aacaatacaa gcagggtgat tcacaatctg gttggcgatc ttcccaagtg gctcgccgaa
    10501 ttccgaatag gagtactaag ctagaatcga ctgcatgaat cgcgacgata taggattgct
    10561 gccggcgatc tcgttcggca ccctgatctt gttttacttc gtttgggccg ctatgcacga
    10621 catagcgcac ggcgatgaag gaacgcttga atggaccgtg ctggccatct gcgctgtggc
    10681 gttcgccgga ctgtaccgcc aagctctttg gttcttaacg cctaaagcca aggtggcatg
    10741 gcttatcggt acgggccttt tgctcgtttt gtacagcgcc ggggctatca gctccatgct
    10801 gcaccccaag tatgtcaagg atccgatgct ggccaggacg ttcttaatgg taggctcgcc
    10861 ggctctcgcg ctcattagct atcacctggt tcgcgaagcg ctgcacagcc gggctcggcg
    10921 cggttggcat cacgcgcaca cgaattcctg atcgttcact aagcagtgtt gcgctaggcc
    10981 ccggagcggg cggccttccg ggccggtttc ttccggcctg tgcggttccc tatatcttcc
    11041 ggataccgca tcgccaagcg acggatctcg taattgaatt tcagaaacag tttttcccag
    11101 cgctttgcct gctcgggtgt gtacttgtaa aagccgcgtc cgttcgaaat gccccgtcct
    11161 ccggcttcaa tgacgccgcg catgagggga gggacatcgg gactcgtgga cagttccggc
    11221 agcaggtctt tcatcacgcg ggcataggct tcgacaccca tcaggtccat atagcggaaa
    11281 ggaccggcga aagtgatcca ccagcccaca tcgttgcgca gcgaccggtc gacgtcttcc
    11341 accgtcgcga tgcccgaatc caccaggtgg caagcctcgc gaaacatcgc ataggaaacc
    11401 cggttggtga tgaagccgcg cacgtcgcgc cgcaccaggg atggctcctt cccccacgcc
    11461 cgggccagtg ccatcacctt gtccgtatgc ttccgctctg tcttctcgcc cgcaatgatc
    11521 tccatgaacc gcgtcacatg agcgggctca tcccaatgga tccccacaaa gcgctcgggc
    11581 cgcaccgcgc cctgttgcag cagggtgaga ggaatggacg aagtgttgct gccaatgatc
    11641 gtgtccccgg ccacgttctc ttccaccacc cggtaggtct gcttcttggt atcgatgtct
    11701 tcgatgatcg attcaatgac gatgccgcac ccggccaggt cggcgaaatc ttccgagatc
    11761 gcgaatttgg gcatcagcga gaccgggtct ttacgcagga gcccctcgcg cttcatttcc
    11821 tgaagcagcg ccaagatatg ccgcggcgtc ccgcgatgtt tcgccgtgct gcgtgtgatg
    11881 gcggttacgc ggtgccccgc cgccagcaga cacgcgatga tgctgtgtcc catcaggcca
    11941 agaccgatga caccagcgcc gatctgccca ggtctgacgg cgtccccgtt gctgggcttc
    12001 ccggccgtat gtttcatatg agtcctcccg cgtttctcaa ttggactagt atacacagac
    12061 cgccgcctgc atttttttga caatgtcctg tgcgaatttt tccggaaaac ggtctgagcg
    12121 cgtccaaagt aagaagatat aagttccaga gggagactat agatgacgac acgactcaca
    12181 gaagcagaac ggcgaaccgc cggttctctc agcgcatatg ggaaagcccg gtccaccgtt
    12241 cagggagcac cgttgacgga ggaagatctg tcgcgcatcg atgcttactg gcgcgcgtgc
    12301 aactacctgg ctcttggcat gatctatctc caggacaacc ctctcctgag ggagccactg
    12361 aaaccggagc acatcaaaaa ccgcctgctc ggacactggg gggccagtcc cggactcgcc
    12421 ttcatttacg ttcacctgaa ccggcttatt cgaaaataca acctcaatat gatttttctg
    12481 gccggtcccg gtcatggcgc cccgggcgtg ttggggccgg tctatctcga aggcacctac
    12541 tcggagatat atccggagaa gagccaggat gaagacggtc tgcgggaatt cttcaaacag
    12601 ttttcttttc cgggcgggat cggcagccat tgtacgccgg aaacaccggg ctccattcac
    12661 gaaggcggcg aactgggcta cgtgctgtcg catgcttgcg gcgcggcgtt cgacaatccc
    12721 gatctgattg ccgcggccgt cgttggcgac ggtgaagcgg aaacaggacc gctggccact
    12781 tcctggcata tcaccaagtt cctcaatccg atccgggacg gtgcggtcct gccgattctg
    12841 catttgaacg gatacaagat caacaacccc acactgctcg cgcgcatttc tcatgaggag
    12901 ttggagcaac tgctgcgggg atacggttgg acgccgtatt tcgtagaagg ctccgacgtg
    12961 gccacgatgc accaggcgat ggcggcgacg gtggagcact gcgtcctgga tatccggaag
    13021 agtcaggacg agtgccggaa aaccgggact cccgcgcgcc ctcgctggcc gatgatcgtg
    13081 ctgcgtacac caaagggctg gagcgcaccg gctgaggtgg acggccaccg tctggaaggc
    13141 ttctggcgcg cgcatcaggt tccgctcgcc ggtgtcaaaa gcaatccgga acatctccgt
    13201 gtgctggaga actggatgcg ctcgatgaag cccgaagaac tgttcgatga aaccggccgg
    13261 ctcatcatgg atctgaggga cctggcaccg gcgggtaatc gccgcatgag cgccaaccca
    13321 catgccaacg gcgggcttgt caagcgatcg ctgcgcctgc ccggcttccg ggattacgcg
    13381 gtgaaggtgg agcggcccgc ggcaatggaa gtcgagaatg ttccgcctct gggaaggttt
    13441 ctggcagacg tgatgaagcg gaacccggat aacttccgcg tttttgctcc ggacgaaaac
    13501 acgtcgaaca agctggatgc gatttatgaa gccacaaaga aattctggat tgccgaatac
    13561 tttccggaag accaggacgg tggcgaactg tctcccgatg ggcgggttat cgagatgctc
    13621 agcgagcaca cgatagaggg aatgctggag ggatatctgc taaccggccg caacggcttc
    13681 ctctcttcgt acgaagcgtt tactcatgtc atcgactcga tgttcaacca gcatgcgaag
    13741 tggctggcga tctcgaacga gctatcctgg cgtgaagaaa tcgcgtccct gaacctcctc
    13801 atcacttcga ccgtttggcg tcaggaccac aacggcttca ctcaccagga tcccggattt
    13861 ctggatatcg ttctgaacaa aagcgccgcg gtgacgcgca tctatcttcc gcccgacgcc
    13921 aactgtctgc tatccgtggc cgatcattgc ctcagaacgg aaaactacgt taacgtcatc
    13981 gtttccgata aacagcgcca cctgcaatat ctggacatcg agttcgcgat catgcactgc
    14041 accaagggaa ttggtatctg ggactgggca agcaccgatc gcgggaccga gccggacgtc
    14101 gtgatggccg cggcagggga tataccaacg caggaggcgc tggccgccac ggctcttctg
    14161 cgggaacagt ttcccgatgt gaagatccgc tttgtcaacg tcgtcgacct tttcaagttg
    14221 cagccggcca cggaacaccc gcatggtttg accgaccgcg acttcgattc tctgtttacc
    14281 gtcgataagc cgattatctt caactttcac gggtatccct ggtcgattca ccggctggct
    14341 tatcgccgca ccaatcaccg gaatctgcac gtacgcggat acaaggaaaa gggcaacatc
    14401 aacaccccgc tggagttggc catcgccaat gaggtggacc ggttcagtct tgccatggac
    14461 gtcatcgatc gtgttcccgc gctacagcgc atcggggccc acgccaagga agagtttcga
    14521 aaccggcaga tcgcctgcaa gaactacgcc tacgagcacg gaatcgatcc gccggaaata
    14581 acggaatgga agtggccgta cgggccgcac ggcgaagttc agaattcgta agtaagcccc
    14641 acgacagcgc gccgcatttc agccggacca taaacataag ctgagtcgcg ggacgggcct
    14701 tgatcgagat ctttctgata actgtcgccg atattctgca tgttaaagaa caggcgaatt
    14761 ttcgaggcat cggtgacggt gaaagtcttg ctgaccacgg cattgaatac cccaaaacgc
    14821 ttgctcgtct cgagccggtc ttcgttgatg taacccgcgt agtgcgggac cagcatggag
    14881 ccggtgaaat ccgccgtgcc ggtcacctcg atctcatggg gcagatccca gtcgaggcca
    14941 aggaatccgt atcgctttgg cgcccggaag aaatcgctgg cgccaaactg ctcttcgggt
    15001 tctttccaac gcgcctgctg cacggtaaac cctccccgaa gaccaaggcg gctgttgagt
    15061 tgaatggtcc cgtcgaaatc cacaccggcc acatgcgcac ccggcccgtt gactctcaac
    15121 aggcggcgga atgtctgacc aggcacctca tcctcgacaa gcgtgaacgc gtggcgcagg
    15181 tctgtataga agat
//
LOCUS       DASTMK010000374         7594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_81883, whole genome shotgun sequence.
ACCESSION   DASTMK010000374 DASTMK010000000
VERSION     DASTMK010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81883"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1194)
                     /locus_tag="VFQ79_16345"
     CDS             complement(223..1194)
                     /locus_tag="VFQ79_16345"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU0141294.1"
                     /translation="MATTYLEAIREGIWEEMERDPNVFVIGEDIGIYGGAFKITAGML
                     DHFGEKRIVDTPISESAIVGAAIGAGMMGLRPVAEMQFADFITCGFDQIVNFAAKCRY
                     RWGASVPIVVRAPSGGGIHGGPFHSQNPEAWFVHTPGLKVIAPSTAYDAKGLVKSAIR
                     DNDPVIFFEHKALYRRVKEDLPQEDYTVPIGKARVFREGSDLSIITYGAMVYVAEEAA
                     TELEKEGISLEIVDLRTLLPIDEETVLESVRKTSRVILLHEDTLTGGIGGEIAARIGE
                     KAFEYLDAPVMRIAAPDTPVPYSPPLEEAFLPNTQKVLEKARQLLGY"
     gene            complement(1196..2284)
                     /locus_tag="VFQ79_16350"
     CDS             complement(1196..2284)
                     /locus_tag="VFQ79_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU0141295.1"
                     /translation="MKWRILQALDDNLALSMTMAAVKSLPAGNPAVAAEESGTEAGLA
                     LRAYHYMTLMREVEDRIERKLYRQGKILGGVYVGRGQEAICIGAGLAAEPEDVLFPSH
                     RDLGLYFIRGIHPRQVFAQYMGRAGGPTRGKDGNMHMGDLSLRIVAIISAMAASVPVA
                     AGAAMALRYKGSRNVALCFFGDGATSRGDWHEGLNLAAVQKAPVVYICNNNQYAYSTP
                     LSKQMCCENVADRAPGYGIPGHIVDGNDVLAVNATVKRAIEHARAGRGPTLIECKSFR
                     MTGHSAHDAASYVPEHLWAEWAKKDPISRLRSRILAEAWAEEQEMDRIVERIRAEVEE
                     AVEWAENSPYPDPSEVMDNVYEQSTEGK"
     gene            2349..3209
                     /locus_tag="VFQ79_16355"
     CDS             2349..3209
                     /locus_tag="VFQ79_16355"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0141296.1"
                     /translation="MPYILSRRNLLRLAAAPAAMALAQDRSSSAKWALLSDVHIPADP
                     SNAYRGFRPVENLKKTVPAVEAFKPDGMIICGDLARLEGLPGDYEALGALVRPVTERT
                     PVAMALGNHDDRKNFLAAFSTRPGEAQDVQGKWVSALDAGPVRIVLLDSLLYVNRVAG
                     LLGKAQRQWLETYLRSNQTPTVFFVHHTLDDTDGAMLDSQRFFEIIRPARQVKAVFYG
                     HSHRYRYDEEDGLHLVNLPAVGYNFNDAEPVGWVEALFRKDGGDFTLRAIGGNTEQSG
                     KTVSLRWRSS"
     gene            complement(3186..3764)
                     /locus_tag="VFQ79_16360"
     CDS             complement(3186..3764)
                     /locus_tag="VFQ79_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEU0141297.1"
                     /translation="MALAKTAVWILALVPAAVLIWKGFRQDLGPNPIEYITHATGDWT
                     LRFLLITLAISPLRKILRFPQLIRFRRLLGLFAFFYGFLHFLTYLLLDQFLDFSAMLR
                     DIGKRPFITAGFASFLLMLPLAATSTAGWIRRLGGKRWQQLHRLVYLSAALGVLHYYW
                     LVKSDIRMPVLYGTILAVLLSARLLLRRAPAK"
     gene            complement(3856..4779)
                     /gene="msrP"
                     /locus_tag="VFQ79_16365"
     CDS             complement(3856..4779)
                     /gene="msrP"
                     /locus_tag="VFQ79_16365"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEU0141298.1"
                     /translation="MLIGKAGEIRSSEITPRDVYFSRRKFLAGGVGLAVGTGALRAAA
                     KLEGLGKSPFSTTEKLTPYDAVTTYNNFYEFGSEKDQPARFAKNFQTTPWTVSVEGHA
                     QKPRTFDLDTILALAPIEERIYRMRCVEGWSMVIPWAGYPLSTLLKQVEPLSKAKYVA
                     FQSYYNSKQMPQARFSGIPFPYVEGLRMDEAMHPLTLLCVGLYGERLPNQNGAPVRIV
                     VPWKYGFKSIKSIAKIKLVEKEPNITWNLANSREYGFYSNVNPNVDHPRWSQAKERRI
                     GEFFKRDTLLFNGYGDNVSSLYAGMDLKKYY"
     gene            complement(4836..6644)
                     /gene="typA"
                     /locus_tag="VFQ79_16370"
     CDS             complement(4836..6644)
                     /gene="typA"
                     /locus_tag="VFQ79_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU0141299.1"
                     /translation="MGRNNILNASIRNIAIIAHVDHGKTTLVDAMLKQSGIFRDNEQV
                     AERVMDSNELERERGITILAKTTGVRYAGVKINIVDTPGHSDFGGEVERALKIVDGVM
                     LLVDASEGPLPQTRYVLMKALEAKLPPILVINKIDRADARIQEVLNEVYDLFIDLDAT
                     EDQLDFPVIYTNAKLGIAKTAPEADGADLRPLFDTILEHIPCPAGDPDGVLQLLVANL
                     DYSDYLGRLAIGRIFSGTLNLGDEAGIVKLDGSLEKTRITKLYSFEGLSRVEERIGRP
                     GDVLAIAGVPGITIGETITSAETPKPLTNIQIDEPTIAMTFTVNTSPFAGREGQYVTS
                     RNLRDRLDKELLTNVSIRVEEAGGPDAFKVMGRGELQLAILVEMMRREGYELAVGKPE
                     ILTKTVNGAVQEPLELLVIDCAENFTGVVIEKLGSRKGKMTKMVNNGSGRVRLEFHIP
                     SRGLIGLRGELLTDTRGTAVMNSLFHGYIPWQGDIPTRPTGSLVADRAGRTTAYAIYN
                     LQERGDIFVTPGAEVYEGMIVGENARDADLNVNIVKEKKLTNMRASTADEAIRLVPPR
                     ILNLEQAIEFVRDDELVEVTPHSIRLRKKTLKANQR"
     gene            complement(6647..>7594)
                     /gene="tatC"
                     /locus_tag="VFQ79_16375"
     CDS             complement(6647..>7594)
                     /gene="tatC"
                     /locus_tag="VFQ79_16375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU0141300.1"
                     /translation="PSAGGSGTPPEPPAPADEEDDDGDEGMLRMSFMEHLEELRSRII
                     KAVAGIGVAFGLSLFFAHDLWLIVSQPAMAALTSLGVNPPKLAQITPMETFNTVWIKL
                     PMLTAIFLASPWILYQVWAFIAPGLYKKERRWAGPFVLCSAGLFILGGLFAYFVAFRF
                     GLTFLLGIGRDINIQPLVSVTEYFNLFVNVTLGVGIVFELPVLIFFLTLLRITNPAFL
                     LRNARYAILVIVILAAIVTPTPDIFNLMIFSVPMVVLFFVGIFASYLLILNREGRSFP
                     WKIAALILLAIVAGAAAIVYVVMSRYGYHFEPHWPFLLK"
BASE COUNT         1535 a         2268 c         2215 g         1576 t
ORIGIN      
        1 ctacggtcac attccgttgt tccaagcggt gcccagacca aacttgctgg caggcttcac
       61 tgccctgttg cggtttacgg cctcgatccg cagccgcgtc tcatcacaac ctacacccac
      121 tttcatctca ggttgtgagc caaaggatca tgcggaactt gctaacgcgc ggctccgttt
      181 ccgagccaca accgccagcg agtggtggcg ttaggaagct tcttaatacc ccagcagttg
      241 gcgtgctttt tccagcacct tttgtgtgtt cggcaggaac gcttcttcga gtggagggct
      301 gtatggaacc ggcgtatcgg gtgcggcaat gcgcatgacc ggcgcgtcca ggtattcaaa
      361 agctttctcg ccgatgcggg cggcgatttc accgccgatg ccaccggtca gcgtgtcttc
      421 atggagcagg atcacgcggg aggtcttcct tacgctttcg agcaccgtct cctcgtcgat
      481 gggcagaagc gttcgcaggt ccacgatttc gagcgagatg ccttccttct caagttccgt
      541 ggcggcttcc tccgcgacgt ataccatcgc gccgtaagta atgatcgaaa ggtcgctgcc
      601 ttcgcggaac acgcgcgctt tgccgatggg aaccgtatag tcttcctggg gcagatcttc
      661 cttcacgcgc cggtacaggg ccttgtgctc gaaaaagatc acaggatcgt tatcgcggat
      721 ggcggacttc accagtcctt ttgcatcgta agcggtggag ggtgcgatca ccttcaagcc
      781 cggcgtgtgg acgaaccacg cttcggggtt ctgggaatgg aaaggtcctc cgtggatacc
      841 gccgccggaa ggcgcacgta caacgatagg gacggacgct ccccatcgat accggcactt
      901 ggcggcgaaa ttcacaatct ggtcgaagcc gcaggtgatg aaatcggcga actgcatttc
      961 ggcaaccggc cgcaacccca tcatgcccgc accgatcgcc gcccccacga tggccgattc
     1021 ggaaatagga gtatccacga ttctcttctc tccgaaatgg tccagcatgc ctgcggtgat
     1081 cttgaacgct ccgccgtaga tcccgatgtc ctcgccgatg acgaagacat tcgggtcgcg
     1141 ttccatctct tcccagatac cttcccgaat cgcttccagg taagttgtcg ccacgttact
     1201 ttccttcggt ggactgttcg tagacgttgt ccattacttc cgaaggatca gggtatgggc
     1261 tgttctcggc ccactccact gcttcctcca cctcggcacg gatgcgttcc acgatcctgt
     1321 ccatctcctg ctcctccgcc caggcttccg ccagaatgcg ggagcggagc cgcgagatgg
     1381 ggtccttctt cgcccattcc gcccacaggt gctcgggaac gtaggatgcc gcgtcgtgtg
     1441 ccgaatggcc tgtcatccga aaggacttgc attcgatgag agtcggcccc cgtccggctc
     1501 tggcgtgctc gatggcgcgt ttcaccgtag cgtttacggc cagaacatcg ttgccgtcca
     1561 caatgtggcc cggtattcca tagccgggag cgcggtcggc aacgttctcg cagcacatct
     1621 gtttggagag gggcgtggaa tatgcgtact ggttgttatt gcagatgtag acaaccggcg
     1681 ccttctgtac cgccgcgaga ttcaggccct cgtgccaatc gccgcggctg gtggcgccgt
     1741 ctccgaaaaa gcagagagct acgttgcggc tgcccttgta ccggagcgcc atcgcggcgc
     1801 ccgccgcaac cggaacggaa gcggccatgg cactgatgat cgcgacaatg cgcaggctga
     1861 ggtcgcccat gtgcatgttg ccgtctttgc cccgcgtggg gcctccggcg cggcccatat
     1921 actgcgcgaa aacctgtctc gggtggattc cgcgaatgaa ataaaggccc aggtcgcgat
     1981 ggctggggaa aagaacgtcc tcgggttccg cggcaagccc ggcgccgata caaatggctt
     2041 cctggcctct acctacgtag acgcccccga ggattttgcc ttgccggtag agcttacgct
     2101 cgatgcgatc ttccacttca cgcatcagcg tcatgtaatg ataggcccgc agcgcaaggc
     2161 ctgcttcggt gccggattct tccgcggcta cggccggatt cccggcgggt aaggatttca
     2221 cagcggccat ggtcatactc agggcgagat tgtcgtccaa agcctgtagt atacgccatt
     2281 tcaccacccc ggcagagaag cgcgagccgg tctggcgccg ccccccgccg ggccgctatc
     2341 atgtcttcat gccgtacatt ctgagccgca ggaatctgct tcgtctcgcc gccgcgccgg
     2401 ccgccatggc tctggcgcag gaccggtctt cttccgcgaa atgggcgctg ttgtcggatg
     2461 tccatattcc cgccgatccc agcaacgcct atagagggtt ccggcctgtc gaaaacctga
     2521 aaaaaaccgt tccggccgta gaggctttca agcccgatgg gatgatcatc tgcggcgacc
     2581 ttgcaaggtt ggaaggtttg cccggcgatt acgaagccct gggggctctt gtccgtcccg
     2641 tgacggagcg aacgcccgtc gccatggcgc tggggaacca tgacgatcgc aagaatttcc
     2701 tcgcggcctt ttccacccgg cccggtgaag cccaggatgt acagggcaaa tgggtctccg
     2761 cgctggacgc cggaccggtc agaatcgtcc tgctggattc cttgctctac gtaaatcgcg
     2821 tggccgggct cctaggcaag gcacagcgcc agtggttgga aacgtacctt cgctccaacc
     2881 agacaccgac ggttttcttc gtgcaccata cgctcgatga caccgacggc gccatgctcg
     2941 acagccagcg ctttttcgag atcatccggc ccgcccggca agtgaaagcc gtgttttacg
     3001 gccattccca ccgctaccgt tacgacgagg aggacggact tcacctggtc aatttgcccg
     3061 ccgtgggcta caacttcaac gacgcggaac ccgttggctg ggtagaggcc ctgttccgga
     3121 aagacggagg cgatttcacc ctccgcgcca tcggcggcaa cacggagcag tccggaaaaa
     3181 ccgtttcact tcgctggcgc tcgtcgtaac agcagccgcg ccgacagcag gacggcgaga
     3241 atcgtgccgt ataaaacagg catccggata tcggacttca ccagccagta gtaatgaagt
     3301 actcccaatg ctgcgctcag atagaccagc cggtgaagct gttgccaccg tttgccgcca
     3361 agacgccgga tccatcccgc cgtcgaagtg gcggcgagag gaagcatcag tagaaacgac
     3421 gcgaacccgg cggtgatgaa cggccgtttc ccgatgtccc ggagcatcgc cgaaaaatcc
     3481 agaaactgat cgagcaatag atacgtgaga aagtgcagga agccgtaaaa aaacgcgaat
     3541 aaccccaaaa gccgtcgaaa acgtatcaat tgcgggaagc gtagaatctt tcgcagcgga
     3601 gagattgcca gcgtaataag gagaaaccgg agggtccaat cgccggtcgc atgcgtgatg
     3661 tattcgatcg ggttgggtcc aaggtcctga cggaagcctt tccagatcag aaccgctgcg
     3721 ggcaccagtg ccagaatcca aactgcggtc ttggcgagcg ccatcacctt agtgaaaacc
     3781 gcttgctcac gcgcgcggct ccgttccgag ccgcgaccgt aagggagcgg accccgaact
     3841 ccctttttca gcatcctaat agtatttttt gagatccatg ccggcgtaga ggctcgagac
     3901 gttatcgcca taaccgttga acaggagcgt gtcgcgttta aagaattcgc cgatccgccg
     3961 ctcttttgcc tggctccagc gtggatgatc cacattcgga ttcacgttgg aatagaaacc
     4021 gtattcgcgc gagtttgcca ggttccaggt aatattcggc tccttttcaa caagcttgat
     4081 cttggcgatg gacttgatgc ttttgaagcc gtacttccac ggtacgacaa tacgcaccgg
     4141 cgcgccgttt tggttgggca gccgctcacc gtaaagtccc acgcagagca gggtgagcgg
     4201 gtgcatcgct tcgtccatcc gcaagccctc cacgtaagga aacgggatgc ccgaaaagcg
     4261 cgcctggggc atttgcttgg agttgtaata actctggaac gccacatact tcgccttgga
     4321 gagcggttcc acctgtttga gaagcgtact cagcgggtat cccgcccacg gaatgaccat
     4381 cgaccacccc tcgacacagc gcatccggta gatccgctcc tcgatgggcg ccagtgccag
     4441 aatcgtgtcc aggtcgaagg ttcgcggctt ttgggcgtgg ccttccacgg aaacggtcca
     4501 cggtgtggtc tggaagtttt tcgcaaacct ggcaggctgg tctttctcgg agccgaactc
     4561 gtagaagttg ttataggtcg tgacggcgtc gtagggcgtc agcttttctg ttgtactgaa
     4621 cgggctcttc cccagtcctt ccagtttcgc ggcggcgcgc agcgcaccgg tgcccactgc
     4681 caggcccact ccgccagcca ggaatttcct gcggctgaaa tatacgtcgc gaggcgtgat
     4741 ctcggaggaa cggatttcgc cagctttccc aatcaacata gcggcatccc cttatctaca
     4801 atgtattcgt ttttgaggcg gcggatgtca ggaatttagc gctggtttgc cttgagggtc
     4861 ttcttccgga ggcggatgga gtgcggcgtc acttccacca gttcgtcgtc acggacgaat
     4921 tcgatagcct gttccaggtt cagaatacgc ggaggcacca gccggatggc ctcatcggcg
     4981 gtggaggcgc gcatgttggt gagcttcttt tctttgacga tgttcacgtt gagatcggca
     5041 tcgcgcgcgt tctcgccaac gatcatgcct tcgtagactt cggcgcccgg cgtaacgaag
     5101 atatcgccgc gttcctgaag gttgtagatg gcgtaggcgg tcgtcctgcc tgcgcggtcc
     5161 gccaccagcg agcctgtcgg cctcgtgggg atatcgccct gccaggggat gtagccgtgg
     5221 aatagcgagt tcatgacggc ggtgccgcgc gtatcggtca gcagttcgcc tcgcaaaccg
     5281 atcaggcccc gggaaggaat atggaactcc aggcgaacac ggccggagcc gttattcacc
     5341 atcttggtca tcttgccctt gcgggatccc agtttttcga tgacgactcc ggtgaagttt
     5401 tccgcgcaat cgataaccag cagttcgagc ggctcttgta ccgcgccgtt cacggtcttc
     5461 gtcagaattt ccggcttgcc caccgcaagc tcatagcctt cgcggcgcat catttccacg
     5521 agaatcgcaa gctgaagctc accgcgtccc atcaccttga atgcatccgg tccccccgcc
     5581 tcttccacgc gaatggaaac gttcgtgagc agctccttgt ccagacggtc gcgcagattg
     5641 cgggaggtta cgtactggcc ttcgcgtccg gcgaacggcg acgtgttcac ggtgaaggtc
     5701 atcgcgatcg tcggctcatc gatctgaata ttcgtgagtg gctttggcgt ctcggcgctg
     5761 gtgatcgttt ctccaatcgt gatccccggg acgccggcga tggcgagcac gtcgcccggc
     5821 ctgccgatgc gctcctccac gcgggacagc ccctcgaagg aatagagctt cgtgatgcgc
     5881 gtcttttcaa gagacccgtc gagcttcaca atcccggctt cgtcgccgag gttcaacgtg
     5941 ccggagaaaa tacgcccgat cgcaagccgg cccagatagt cgctgtaatc cagattggcg
     6001 accagcagtt gcaggacgcc gtcagggtca ccggcagggc agggaatatg ttcgaggatc
     6061 gtatcgaaaa gcgggcgcag atccgcgcca tccgcctccg gcgcggtctt tgcgatgccg
     6121 agcttggcat tcgtatagat gaccggaaaa tcgagctggt cctcggtggc gtcaaggtcg
     6181 atgaacaggt cgtagacttc gttcaatact tcctggatgc gtgcatcggc ccggtcgatt
     6241 ttattgataa ccaggatggg aggcagcttc gcctcgagcg ccttcatgag cacatagcgg
     6301 gtttgcggaa gggggccttc gctcgcatcg accagcagca tgacgccgtc gacgatcttg
     6361 agcgcccgct ccacttctcc gccgaagtcg ctgtgacccg gcgtatcgac gatgttgatc
     6421 tttactccgg catagcgcac gccagtggtc ttggcgagga tggtgatgcc gcgctcgcgc
     6481 tccagttcgt tggaatccat aacgcgctcg gccacctgtt cgttgtcgcg gaagatgccg
     6541 ctctgtttca gcatggcgtc gacgagcgtg gtctttccgt gatcgacgtg cgcaatgata
     6601 gcgatattac gaatggatgc gttgagaata ttgtttctcc cgatgcctac ttaagcagga
     6661 aaggccaatg cggctcaaag tgatagccgt accggctcat gacaacatag acgatggccg
     6721 ctgctcccgc tacgatcgcg agaagaatca gcgcggcgat cttccacggg aaactccgtc
     6781 cttcccggtt aaggatgagc aggtagctcg caaaaatgcc gacgaagaag agcacgacca
     6841 tcggcacgga aaagatcatc aaattgaaaa tgtcgggtgt cggcgtgacg atggctgcca
     6901 ggatgacgat caccagaatc gcgtagcgcg catttctcaa caggaacgca ggattggtaa
     6961 tgcgcagcag ggtgagaaaa aagatcagta ccggtagctc aaaaacaatt ccgacgccga
     7021 gcgtaacgtt cacaaacagg ttgaaatact cggttaccga gacaagcggt tgaatgttga
     7081 tgtcacggcc aatgccgagc agaaacgtca atccgaaacg aaatgcgacg aagtaagcga
     7141 acagcccgcc caggatgaac aacccggcgg aacacaaaac aaatgggccg gcccagcgcc
     7201 tctccttttt atagagaccc ggcgcgataa aagcccacac ctgatacaaa atccacggcg
     7261 acgccaggaa aatcgcggtc agcatcggca gcttaatcca caccgtgttg aacgtttcca
     7321 tcggggtgat ctgcgccagc ttaggagggt ttactcccag cgacgtcagg gccgccatgg
     7381 cgggctggct gacgatcagc cacagatcgt gcgcgaaaaa caggctgagg ccgaaggcga
     7441 caccgatgcc ggccaccgcc ttgatgatcc gggaccgaag ttcctccagg tgttccataa
     7501 aggacatccg gagcatcccc tcgtcgccat cgtcgtcctc ttcatcggcc ggagctggag
     7561 gctcgggggg cgtgccggag ccgcctgcgg aggg
//
LOCUS       DASTMK010000375        13960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_274997, whole genome shotgun sequence.
ACCESSION   DASTMK010000375 DASTMK010000000
VERSION     DASTMK010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274997"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2961
                     /locus_tag="VFQ79_16380"
     CDS             <1..2961
                     /locus_tag="VFQ79_16380"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0141301.1"
                     /translation="RVWTHVEKSTRLIGLQGSLLLNRFLWIGIALGVLALTHLRFRFA
                     HHTAGAWWSRGAQRTDQELSLAYARASEPSCDREGAVRRTFGFTTHTRQVLAVARESF
                     QEIVMSWGGFVLVGMAALAAVSASQIEHMGVPLFATAERMTAFLAAPLTNPQEFFGMM
                     VPLFILIYAGELVWRERETLLGEITGAAPVPEWVFFLGKFVGLGLVLVTLQALMMAAG
                     ILIQVLLGHFDFEIGLYARILFGLQLPDYLLFALLALVVHVVVNHKYVGHLVVVLAYA
                     FMAFGPALGVGHNLLVYGSDPGWTYSDMRGFEPFIGPWLWFKFYWAAWALLLAVAATL
                     LWVRGKEADLGSRLALARRRLTRRAARVAGAAGALILTLGGFIFYNTNVLNAYQTVSD
                     GLAWRAKYERRYGQYKNIPQPQLTAINLNVEIYPEHRAAEIRGTFHLVNTSAAAIDSI
                     HLATKPAVNTTAVRFDRPGRNVRSDEEFGHQIYALETPLRPGESLQLDFEVHFKPRGF
                     PNRGMDASVVANGTYFTNDAWLPAIGYQADRELRSAGDRRKYRLAARPETPMLDDVEA
                     RRDAGGDARIAFEAVVGTGEAQVAVAPGRLRRTWTQSGRRYFHYATDVPIRNHYAFFS
                     AAYAVHEARWNDTSIEIFHHPAHAWNVDRMARSIQASLNYYTQQFGRYPHGQLRFVEQ
                     PGDDVILHASPVNISYEEGFSLLNPGADPRDIDLPFAVVAHEVAHQWWGATLTPTRVE
                     GAAVLTESLAWYSALQVVERTFGREHLRRLLGMMREVYATPRTRAEVPLLRATDSFLT
                     YRKGPFAMYALREYAGAGQVNAALRRLLERHGSGTPPLATTLDLYRELKAVIPEPFHS
                     LLADLFERNTFWELATQRVNTKQTGTGTWQVTLDVKTRKVVVDEAGVETEVPMDDLVE
                     VGVFADAEDGGFGDPLYLRMHRIRSGEQRITVMVASKPARAGIDPRSLLIDVETDDNL
                     KEITRAEGVRSRGR"
     gene            complement(3011..3907)
                     /locus_tag="VFQ79_16385"
     CDS             complement(3011..3907)
                     /locus_tag="VFQ79_16385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0141302.1"
                     /translation="MTIRSDQRTTTDRIVHMNAVSRGFFAALGTRFVAGNDFDERDAL
                     PVSEGGRRVAIVNEAFVKRYLGGRSPLGVHIAGGTGPDVNPQTEIIGVVADISYRGVR
                     EEWEQAYFPIRNDEDFYLGANFYLRMRGASQAGFQSVRTILQRVDPALPIDYLRTLDE
                     QVSRSLNTERMLAALSASFGTFALLLSLVGLYGVMSFVVTQRTREIGIRLALGSTRLG
                     AVWLVLRDALGMIAVGIAVALPCVWMLGRLVESQLYDIKPTDPATIAVATLILCSAAL
                     GAALIPARRASTVNPTEALRFE"
     gene            complement(4020..5723)
                     /locus_tag="VFQ79_16390"
     CDS             complement(4020..5723)
                     /locus_tag="VFQ79_16390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141303.1"
                     /translation="MKRLLNWFRRGKLESDLDRELEYHIERRVSDLMLSGLPEPEARR
                     QATLEFGGVTQVQEEVRDIWLTRWLRDFAYDLRFSARSFLRSPSFTATAVISLALGIG
                     ATIAIYSLLDQVVRRALPVREPERLVLIDWIGDQASVNAFGSTNLMSYPICDDLHTQV
                     RFFEGVFCRAATSVSLSTGKEARLAAAEIVSGTYFSVLGVTPAVGRLLTPDDDQAPGA
                     SPVVVLSYDFWKAHLAGDPNIIGRKVLVNQHPMTVIGVAAAEFRGIDVGAVPALWIPA
                     AMSAGAIPGFDGLLNRRAKWMQVLGRLRPGVSLTQAQAGLQPWFKAMLEEDTHRAGFP
                     KITPERRRQFLASTIELTPAPRGHSFLRSRLSQPLWVLTAATVVLLGLACLNVAGLFL
                     ARASARDHEISTRLALGASRGRIGRQLLADSVLLALAGGSLGVALAPFAMRALIAFLP
                     ADVASTAVESSVDARLLLFTFLVSAAAGILTGFAPAIQTGRRPLITSLRERGGSDFGG
                     VRLRRIIVTAQIAFTLVLVIGAALFVRTLAGLMAKGPGFDTSSLISFGVHPVRKRLLS
                     L"
     gene            complement(5726..6061)
                     /locus_tag="VFQ79_16395"
     CDS             complement(5726..6061)
                     /locus_tag="VFQ79_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141304.1"
                     /translation="MPPTRFPIPQGTLDMLILQILSLEPAHGYGIAQRLEQISRSVVR
                     VNQGSLYPALHRLEQRGWLKSEWKQSETGREAKFYSLTPSGRKQLKAEKESWARLTGA
                     VQLVFDESK"
     gene            6193..7542
                     /locus_tag="VFQ79_16400"
     CDS             6193..7542
                     /locus_tag="VFQ79_16400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0141305.1"
                     /translation="MSEQADGALVRRVQSGEPAAAGELFKRYWRSARAAAYGVTGDWL
                     AAEDAASEGLSKAIAGIGSLADPDRFAPWLRTIVVRTARMGLRDPSPIEGVTECLRDP
                     RPNPDECLQRQQLIAAVQQAMRKLPETLREAIALHYFEGYDSAGAAHFLDIPTGTLRR
                     RLHEGRKRLRVEVERVLERRKPMGDNVQPQLEKFEAMIREGDAGRALRKIFALQRPPA
                     PLLNLLRNNLGGRRDAGEFARTAASILESQPSHASDPETPVGAMAHAIRVALPEFQEW
                     QIDIVTAATRLFGTGKYRDRLQAVLPPVFAKGHPGNFVKSGWALLKQSEAGIQSVYEV
                     LREDTDEPAFDRSQVVMSEVLDLTWMVDGSLELHSVHQRIAQLISEVLDGAEPAFSSY
                     YEPRYRSAFQIHFAGVFARAAVGGVLAEWPGRPHGVDAAHVRIFLEPWALLRSGQHG"
     gene            complement(7569..8945)
                     /locus_tag="VFQ79_16405"
     CDS             complement(7569..8945)
                     /locus_tag="VFQ79_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0141306.1"
                     /translation="MSGKSLPDQPNLDPVAAFIEAACVPLDGHNSGTLDEAGMILARY
                     PNVAEASIYTAAILADEANIRAYLARDAAGATEKGGPRGWDALTYLCFSRYLRLDRTR
                     SDAFVRTARALLDAGASANTGWIEMIDHPNPRPVLESAIYGAAGIAQHAELTRLLLER
                     GADPNDEETPYHVVETHDNTVLRILLESGKLNARSLSILLLRKCDWHDPEGVRLVLEH
                     GANPNLITDWGLSALHQAVRRDNSLTIIEYLLDYGADSSLPDRDGFSAIAIAAHRGHG
                     AALDLFERRGAAIPLAGVDALLAACARDRQETILSLITAEPQLRTLIIEHGGTLLAQF
                     AGVGNLAGVRTLLDLGVSPAALYREGDGYFAIAKESTALHVAAWRAWPEVVKELIARG
                     APVNAQEAQGRTALQLAVKACVDSYWTERRSPDSVRALFEAGASTAGIELPTGYDEID
                     KLLRGIPT"
     gene            complement(8963..11380)
                     /locus_tag="VFQ79_16410"
     CDS             complement(8963..11380)
                     /locus_tag="VFQ79_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141307.1"
                     /translation="MFRRGFGSVVLLLFFAIATAKAQLPPGLQISSLTPGSVPASILG
                     VSLQVDGTGFRGPSICLTVCGVACPRASVVQIDGSDLPTIFVSGTRLIASVPGVLLGT
                     PRAASIRVHNRTDILCETGGTSTFSNTRLFTITMPPPNVTPSPSTVEMDTNAGSTTPV
                     SFPVTLTNNGSLPAAFSVSTNVPFLSTSPRTGSIAVGQQIDLAVIANPNGLRAGTYTG
                     SVTIRVQNAGESGGQIQTISLPTTFSVHGTVLSFDPPGPLRLTAKVNEVATANLRLRD
                     LAGGTTVAVAANADIGAWLKVATLPASGEAPFLFTVAADATGLMPDVYFGTVSGASIA
                     GSPALEERLAVEFTVTAISTRRTIPQIADGGAFKTSITLVNADKVPATVSLVFRRSDG
                     PDSRTTQPWNIAIANNQPIQNVTIQPGNAYTVETAALENFTSIGWAEVISEQNVTGFA
                     VYRQRLPGQPDQEAAVPVNQGDMQRFLLPFDNRAGFRTTMAIANLSVTENAVVNVAFR
                     DASGVLIGGGTVKTIPPQGHYAYRLIDDFPIDGQEGVAEFTAVSGQISAVGLRFNPTS
                     SFTSFEVQRFAPPAGGVRRVIPQIAEGGVYTTAITLVNTDTFPAVVSLRFHRSNGPDV
                     RTTQPWNPPIVGNRPTENVILQPGNVYTVQTAGQGNFTSIGWAELITNQAVSGFAVYR
                     QRIPGIADQEAAVPVNTESMQSFLLPFDNRDFFRTTMAIANLSTTEQALVQVVFRDTK
                     GVPLGSALVKTLPPQGHYAFRLIDDFGFDGVQGVAQFTALSGQISAVGLRFNPTNAFT
                     SFKVQPN"
     gene            complement(11473..>13960)
                     /locus_tag="VFQ79_16415"
     CDS             complement(11473..>13960)
                     /locus_tag="VFQ79_16415"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141308.1"
                     /translation="ILGASGSGKSSLARAGVLYQIQKGALPGGKIWPVIAFKPAANPL
                     ESLSIELLRHAHAETTGKALAEMIDGFLHDERRLHLSIAAALLNQPAGTRMALLVDQF
                     EEVFTVCRDEKAREAFIKNLLYAASAGGPSVVLLTLRADFYGECAGFVGFASALESRH
                     VLVGGMAEGELRAAIEEPARIANVDMEPGLADVLVREASGQAGALPLLQYTLMRLWEE
                     CAVSGRLSLDAYRGMGGLSGALHTQAENVFTEWLSNSQPEISRRVFLRLAEAQPDGRY
                     TRVRTAISQLHPASAAPEQVKNIEEVVGILAGPKARLLTVSSGQAAGPTVEVAHEELF
                     RAWKRYSQWLEEDRAFLNWRRTFGVGLSEWLRLGRDPDCLLRGALLKEAEGWFAERAE
                     DFTETEAAFLRSSREAVDAEARAAQAAKRRRYLLIAGQIAASIALVLVIWLFISRAQV
                     TGKLLAKAKELRASDGKLALLMAYQANLRRDGPETLSALYTSLEAATAAEIEGHEAIA
                     ISFSPDGNYLATGAADGTVALWDADVAHGDQTYSELRRLSNEERLRLDGPVAAVRFSN
                     ANVLAAGTLRGIVGGFNPDTGALLPPVLLKQSGITSIAISPDGARILTGAEAGEIRLW
                     SATGAGLEQYRIPAERVSAVTFSGDGRRFAAGGSNGVFAVWDTGSPAPIWEQKDPDGT
                     FSFLALPADSRWLMTASRSGKARRWDLETKSEERVFSPPTGLVRLAAVAEDGSRLATQ
                     TDGNSIVLWDATGGNELFTISSDAAPVRAMAFSFDAKRLAVARTDGTARIMELDRNRV
                     RTRVLTILRRDTRFPFTNECREAAGSCSGL"
BASE COUNT         2770 a         4311 c         4304 g         2575 t
ORIGIN      
        1 agggtatgga cgcacgtcga aaaaagcacg cgtttgatcg gactgcaggg ctcgctcctc
       61 ttgaaccgtt tcctttggat cggcattgcc ctgggcgtgc tcgcgctcac gcacctccgg
      121 tttcgcttcg cgcaccacac cgcgggcgcc tggtggagcc gcggcgcgca gcgcacggat
      181 caggaactat cgctggctta cgcgcgcgcc tcagaaccga gctgcgaccg tgagggagcg
      241 gtccggcgga ccttcggttt cacgacgcac acgcgccagg tgctcgccgt cgcgcgggag
      301 tctttccagg agattgtgat gagctggggc ggctttgtcc tggttgggat ggcggcgctc
      361 gcggcggtca gcgccagcca aatcgaacac atgggcgtgc cgctgttcgc gacggccgag
      421 cgcatgactg cgttcctggc tgcacccttg acgaatcccc aggaattttt tgggatgatg
      481 gtcccgctat tcatccttat ttacgcgggc gaactggtct ggcgggaacg ggaaacgctc
      541 ctgggcgaga tcaccggcgc tgcgccggtc ccggaatggg tcttttttct gggcaagttc
      601 gtgggacttg gtctggtgct cgtcacgctg caggccctca tgatggcggc ggggatactc
      661 atccaggtcc tcctgggcca cttcgatttc gagatcggcc tgtacgcgcg gattcttttc
      721 gggcttcaac tccccgacta cctcctcttc gccctgcttg cccttgttgt tcacgtagtg
      781 gtgaatcata agtacgtcgg acatctcgtg gtcgtcctcg cctacgcgtt catggccttt
      841 ggcccggcgc tgggagtcgg gcacaacctg ctcgtctacg gatccgatcc tggctggacc
      901 tactcggaca tgcgcggctt cgaaccattc atcgggccgt ggctctggtt caagttttat
      961 tgggctgcat gggcgctgtt gctggcggtg gcggcgacgc tgctctgggt acgcggcaag
     1021 gaggcggacc tcggctcgcg gctcgcgctg gcgcgccgcc gcctcacgcg tcgagccgcc
     1081 cgggtcgccg gggcggcagg ggcgctcatt ctgacgttgg gcggttttat cttctacaac
     1141 acgaacgtcc tgaacgcata tcagacggtc tccgatggcc tggcatggcg cgccaaatat
     1201 gagcggcgct acggacagta caagaacatt ccgcagccgc aactgacggc catcaacctc
     1261 aatgtggaaa tctatccgga gcaccgggcc gcagagatcc gcggcacgtt ccatctcgtg
     1321 aacacgagcg ccgccgcgat cgactctatc catctcgcca ccaagccggc ggtcaacaca
     1381 acggccgtcc gcttcgaccg gccaggcagg aacgtacgtt ccgatgagga gttcggccac
     1441 caaatttacg ccctggagac accgctccga cccggcgaat cgctgcagct tgatttcgag
     1501 gtgcatttca agccccgtgg gttccccaac cgcgggatgg acgcctcggt ggtcgcgaac
     1561 ggtacctact tcacgaatga cgcgtggttg cccgccatag gctatcaggc ggaccgcgaa
     1621 ctgcgaagtg ccggcgaccg cagaaagtat agacttgctg cgcgccctga gacgcctatg
     1681 ctcgacgacg tagaagcacg gcgcgacgcg ggaggagatg ctcggatcgc gttcgaggcg
     1741 gtcgttggca cgggcgaagc tcaagtcgcc gtcgcgcctg ggaggttgcg ccgaacgtgg
     1801 acgcaaagcg gacggcgcta ctttcactac gcgaccgatg ttccgatccg gaatcactac
     1861 gcgttcttct ccgcggccta cgccgtgcac gaagcgcggt ggaacgatac ctcgatagag
     1921 atctttcacc atccggccca tgcgtggaac gtggaccgca tggcccgaag catacaggcg
     1981 tcgctgaact actacacgca gcagttcggc cggtacccgc acggccagct caggttcgtc
     2041 gagcaacctg gcgatgacgt catcttacac gcttctccgg tcaacatctc gtacgaggaa
     2101 ggtttctctc tgctgaaccc cggcgccgat ccgcgggaca ttgatcttcc tttcgcagta
     2161 gtggcgcatg aagtggcgca tcagtggtgg ggcgccacgc tcacacccac acgcgtcgag
     2221 ggtgccgcag tgctcaccga gagcctggcg tggtactcgg cgctgcaggt cgtcgagcgg
     2281 accttcggcc gcgagcactt gcggcggctc ctgggcatga tgcgggaggt ctatgcaacg
     2341 ccacgcacgc gcgccgaagt cccgctgctc cgcgccaccg attcgttcct cacgtatcgc
     2401 aagggcccgt tcgcgatgta cgccctgcgc gaatacgccg gcgccgggca agtaaacgcc
     2461 gcgctacggc gcttgctcga gaggcacggc tcgggaacgc cgcccctggc aaccactctc
     2521 gatctttacc gggaactcaa ggcagtcatt ccggagccgt tccactctct gcttgccgat
     2581 ctcttcgaaa ggaacacgtt ctgggagctc gcgacgcaaa gagtaaatac gaagcaaacc
     2641 gggacaggca cctggcaggt gacgctcgac gtgaagacgc gcaaggtggt ggtcgacgaa
     2701 gcaggcgtag agaccgaagt tccgatggac gacctcgtcg aagtcggcgt cttcgcggac
     2761 gcggaggacg gagggttcgg ggatccgctg tacctgcgta tgcatcgcat ccgctccggc
     2821 gagcagcgca tcaccgtgat ggtggcgagc aagcccgccc gggcggggat cgacccgcgg
     2881 agccttttga tcgacgtaga gaccgatgac aatctcaagg agataacacg cgccgagggt
     2941 gttcgatcgc gcggtcggtg acctcctcag caccggcaag caccaagccc caagcgatag
     3001 gaataactgg ttactcaaat cgcaatgctt cggtggggtt cacggtcgag gcgcggcggg
     3061 cgggaatgag ggctgcgccc aatgccgcag agcacaaaat caaagtagcc acggcgatcg
     3121 tagccggatc ggtcggcttg atatcgtaca actgcgattc aacgaggcgc cccagcatcc
     3181 agacgcacgg cagagcgacg gcgattccca cggcgatcat cccgagcgca tcacggagta
     3241 ccagccagac tgcgccaagc ctggtggacc ccagcgccag ccgtatgccg atctcgcgcg
     3301 tgcgttgcgt taccacgaag gacatcacgc cgtaaagacc taccagggag agcagcagcg
     3361 caaacgtgcc gaagctggcc gaaagcgcgg ccagcatgcg ttccgtattc aaagaccggc
     3421 tgacctgctc atccaatgtg cggaggtaat cgatcggcaa tgccggatcg acgcgttgca
     3481 gaatggttcg aacggattga aagcccgcct gggaggcgcc gcgcatcctc agatagaaat
     3541 tggcgcccag gtagaagtcc tcgtcgttgc gtatcgggaa gtaggcttgt tcccattcct
     3601 cgcgaacgcc acgatagctg atgtccgcca ccacgccgat gatctccgtc tgaggattga
     3661 cgtccggtcc cgtacctccg gcaatgtgca caccgagagg gctgcgcccg cccaggtagc
     3721 gcttcacgaa cgcctcgttg acaatggcga cgcgccggcc tccctcgctc accggcagcg
     3781 cgtcgcgctc gtcgaagtca ttgcccgcga cgaagcgggt tccaagtgcg gcaaagaatc
     3841 cgcgggagac cgcattcatg tgaacgatgc gatccgtcgt ggttcgctgg tcgctccgaa
     3901 tcgtcatgaa gttattccag cttccaccgg tcagcaactg aatactcgca agcgctgccg
     3961 actcagtggt actggaggca cgtatctcgt cgtaaattcg gcggacgagg cggcttgcct
     4021 cagagggaga gtagccgttt gcgaaccggg tggacgccga acgagatcag gctggaggtg
     4081 tcaaaccccg gccccttggc catcaacccg gcaagggttc gaacgaacaa ggccgctccg
     4141 atgacaagga ccagcgtgaa agcgatttgg gccgtgacga tgatccttcg aaggcggacg
     4201 ccgccgaagt cagagccgcc gcgttcccgg agtgaagtga tcaacggccg ccgtccggtt
     4261 tggatggcag gagcaaatcc tgtcaggata cccgccgccg cgctgacgag aaatgtgaaa
     4321 agcaacaggc gcgcgtccac ggaggattcg accgcggtcg aggcgacatc ggcgggcaaa
     4381 aacgcgatga gcgcgcgcat tgcgaaggga gccagagcaa cgcccagcga cccgccggca
     4441 agtgcaagaa gaacgctatc ggccagaagc tgccggccga ttcgcccgcg ggacgcgccc
     4501 aatgcgagcc gcgtactgat ctcgtggtcc cgagccgacg cgcgcgctag gaaaaggccg
     4561 gcgacattga gacaggccag tccgagcagc accaccgtag ccgcggtcaa gacccatagt
     4621 ggttgcgaga gcctgcttcg taaaaaggaa tggcctcgtg gagccggagt aagctcgatg
     4681 gtcgaggcaa gaaactgccg gcggcgctcc ggcgtgattt tagggaatcc ggcgcgatgt
     4741 gtgtcttcct ccagcatcgc tttgaaccag ggctgcaagc cggcctgtgc ctgagtcaac
     4801 gaaacgccgg gacgcaagcg gcccagcacc tgcatccatt tcgcccgccg gttgagtagg
     4861 ccatcgaagc cggggatggc tcccgctgac atcgccgctg gaatccatag tgcgggaacc
     4921 gcgccgacat cgataccgcg aaattctgcg gcagcgactc cgataaccgt catcgggtgc
     4981 tggtttacga gcaccttgcg gccgatgata ttcggatcgc cggcgagatg tgccttccag
     5041 aagtcgtaag acagcacgac cacgggactg gcgccgggcg cttgatcgtc gtccggcgtt
     5101 aagagtctgc cgacggcggg agtgactccg agcaccgaaa agtaagtgcc tgacacaatc
     5161 tcggcagccg cgagcctggc ctccttgccg gtcgacaggc ttacagacgt ggcggcacgg
     5221 cagaagacgc cctcgaagaa tcgcacctgc gtgtgcaggt cgtcgcagat cgggtaggac
     5281 atcaggttcg tgctgccgaa cgcgtttacg ctagcttggt caccgatcca gtcgatgagg
     5341 acgaggcgct cgggttcccg taccgggagg gcgcggcgga cgacctgatc cagaagagag
     5401 tagatggcaa tggttgcgcc gattccgagc gcgagtgaga ttacagccgt agccgtaaac
     5461 gagggactcc ggaggaagga gcgggccgag aagcggaggt cgtaggcgaa gtcgcgcagc
     5521 catcgggtga gccaaatgtc gcggacttcc tcctgaacct gcgtgacgcc gccgaattcc
     5581 agggtggcct gccttctcgc ttccggctcc ggcaaacccg aaagcatcag gtcactcacg
     5641 cgccgctcga tgtgatactc cagttcgcgg tccaggtcac tctcgagctt tccgcgccgg
     5701 aaccagttca gcaaccgctt catggtcact tgctctcgtc aaaaaccaac tgaaccgcac
     5761 ccgtaagccg ggcccagctc tccttctcgg ctttgagctg ctttcgtccc gaaggtgtta
     5821 aagaatagaa cttcgcttcg cgtcccgtct ccgattgctt ccactcggat ttgagccatc
     5881 ccctctgctc caacctgtgg agagcaggat aaagcgatcc ctggttgact cgaacgacgg
     5941 atcgggagat ctgctccagc cgctgcgcga tgccgtagcc gtgcgccggt tcgagagaga
     6001 gaatctggag aatcaacatg tcgagcgtgc cttgcgggat gggaaaccgg gtgggtggca
     6061 tagatacctc aaacttctac cattcagcat aaagcataat aggtcgaaga tcaaggtatc
     6121 tttggcgctg aattaccacc cgcgaatttt ttccgaacaa aatagccaca agaccccaca
     6181 taccattgag agatgagcga gcaggcagat ggcgcgttgg tacggcgcgt tcagtccgga
     6241 gagccggcag cggcagggga actgttcaag cgttattggc gaagtgcacg tgcggcggct
     6301 tatggagtca cgggagactg gctggcggct gaagacgcag catcggaagg cctctctaag
     6361 gccatcgctg gaataggctc gcttgcagat ccggaccgat tcgcgccgtg gctgcggacg
     6421 atcgttgtcc gtactgcccg gatgggactt cgggacccat cccctatcga gggtgtcact
     6481 gaatgcttgc gagaccccag accaaacccg gacgagtgct tgcagcggca acaactgatc
     6541 gcagcggtcc agcaagcgat gcgcaaactt ccagaaacac tacgtgaagc aatcgccctg
     6601 cactacttcg agggctatga ttcagccggc gcagctcact ttctcgatat cccaacgggt
     6661 acgctacggc ggcgcctcca cgaaggccgc aagcgtttgc gtgtggaggt ggaaagagtt
     6721 cttgaaagaa ggaaacccat gggcgataac gttcaaccgc agttggagaa attcgaagcg
     6781 atgatccggg agggcgatgc cggtcgcgcg ttgcgaaaga tcttcgcgtt acagcggcct
     6841 cccgcgccat tgctcaacct gctccgcaac aacctaggcg gacgaagaga tgccggcgag
     6901 tttgcacgca ccgctgcctc gatcctggag tcgcagccga gccacgcctc cgatccagag
     6961 actccggttg gcgctatggc acatgcaatc cgagtggctt tgcctgagtt tcaggaatgg
     7021 cagatcgata ttgtcacggc agcgacgcgg ctgttcggta ccggcaagta tcgcgaccgg
     7081 ttgcaggcgg tgttgccgcc ggtattcgcg aaaggacatc caggaaactt cgtgaagtcc
     7141 ggctgggcgc ttctcaagca aagcgaagcg ggaattcaat cggtgtacga agttctgcgg
     7201 gaagacacgg acgagcccgc ttttgaccgc agtcaagttg tcatgtcgga ggttctcgat
     7261 ctgacctgga tggtggatgg ctcgctggaa ctacactctg ttcaccaacg gatcgcgcag
     7321 ttaatctcag aggttctcga tggcgctgag ccggcttttt cgagctacta cgagccgcgt
     7381 taccgctccg cattccaaat tcacttcgcg ggcgtattcg cccgtgcggc tgtaggtggc
     7441 gtcctggccg agtggcctgg gcgtcctcac ggagtagacg cggctcacgt ccgcattttc
     7501 ctggaaccct gggctttgct ccgctccggg caacacggtt gagtcaccct ctggtccaaa
     7561 tgatcggact acgtcggaat gccccgcagc aacttgtcga tctcgtcata ccctgtgggt
     7621 agttcgattc ctgcggtcga cgcgccggct tcgaacaggg cgcggacgga atcgggtgag
     7681 cggcgctcgg tccagtagga atcgacgcag gctttgacgg cgagttggag agccgtgcgg
     7741 ccttgggctt cctgggcgtt gacaggcgcg ccgcgggcga tcagttcctt gacaacttca
     7801 ggccacgcgc gccacgcggc gacatgcaga gccgtactct ccttcgcgat ggcaaaatac
     7861 ccgtcgcctt cacgataaag cgcggctggg ctcacgccca ggtccagcag ggtacggaca
     7921 cccgcaaggt tccccacacc ggcgaattgg gcgagaagcg taccaccgtg ctcgatgatt
     7981 agcgttctca gttgtggctc ggccgttatc agagaaagaa tcgtttcctg ccgatcgcgc
     8041 gcgcaggcgg cgagcagcgc atcaacacct gccaggggga tcgcggcacc gcgccgttcg
     8101 aagagatcga gcgcggcacc gtgtccacgg tgagcggcaa tggcaatcgc agagaacccg
     8161 tcacggtccg ggagcgagga atcggcccca tagtcgagca gatattcaat gatggtgagg
     8221 ctattgtcgc ggcggaccgc ctgatgcagc gcgctgagtc cccagtccgt tatcaggttc
     8281 ggattcgcgc catgttcaag tacgagacgg acaccctcgg gatcatgcca atcgcacttg
     8341 cgaagaagca gtatactcag gctccgcgca tttagcttgc cgctctccag cagaatcctc
     8401 agtaccgtgt tgtcgtgcgt ttccactaca tgatacgggg tctcttcatc gttaggatcg
     8461 gcgccgcgct cgagaagcaa acgcgtcaat tccgcgtgtt gcgcgatccc ggccgcgccg
     8521 tagatggcgg attcaaggac gggccgggga ttcggatgat cgatcatttc gatccaaccc
     8581 gtgttggcgc tcgcgccggc gtcgagcaga gcgcgggctg tgcggacaaa ggcatcggaa
     8641 cgcgtgcggt ccagcctgag gtagcgcgag aagcacagat aggtgagcgc gtcccagccg
     8701 cgcggcccgc ctttctcagt cgcgccagca gcatcgcggg cgagatatgc gcggatgttg
     8761 gcctcgtcag caagaatggc agcggtataa atgctggctt cggcgacgtt cgggtaccgc
     8821 gcgaggatca tcccggcttc atcgagagtg cccgaattat gcccgtcaag aggcacgcat
     8881 gcagcttcga taaacgccgc gacgggatcg aggttgggtt gatcgggtaa agacttgccg
     8941 gacacacgaa gctccctggc cctcagttgg gctgtacttt aaaagatgtg aaggcgttcg
     9001 tcgggttgaa acgcagaccg accgccgaga tctgtccgct caaggctgta aattgcgcta
     9061 ctccctgcac gccatcgaac ccaaaatcat cgatcagccg gaatgcatag tggccttgcg
     9121 ggggtaaggt tttcaccagc gcgctgccca gcggaacacc cttcgtatcg cggaagacca
     9181 cctgaaccag agcctgttcg gtcgtgctga gattggcgat ggccatcgtg gtccggaaaa
     9241 aatctctgtt atcgaacggc agcagaaaac tctgcatgct ttctgtattg accggaacgg
     9301 cggcttcctg gtcggcgatg cccggtatgc gctgccggta caccgcgaac ccgctcacgg
     9361 cctggttcgt aatcaattcc gcccagccga tagaggtgaa gttgccctgt cccgccgttt
     9421 gcactgtgta cacgttgccc ggttgaagga tgacattttc ggtaggccgg tttcccacaa
     9481 tgggcggatt ccagggttgc gtcgtgcgca cgtccggacc attggacctg tggaatctca
     9541 ggctcacgac agcgggaaag gtatccgtat tcaccagggt gattgcggtg gtatatacgc
     9601 ccccctccgc gatttgcgga atcacgcgcc gtacccctcc cgcaggaggc gcaaaacgct
     9661 gcacttcgaa cgaagtgaag gacgatgtcg gattgaaccg cagtcctacg gcggagatct
     9721 ggccggatac ggcggtgaat tccgctacgc cctcctggcc gtcgatcggg aaatcgtcga
     9781 tgaggcggta cgcatagtgg ccctgcggcg gaatggtttt gacggttcca ccgccgatga
     9841 gaactcccga tgcatcgcgg aacgccacat tcaccaccgc attttcagtg acgctaaggt
     9901 tggcgatcgc catggtggta cgaaagcccg ccctgttatc gaacggcagc agaaagcgct
     9961 gcatgtcgcc ctggttgacc ggcaccgcag cctcctggtc cggctggccc ggcagacgct
    10021 ggcggtaaac cgcaaacccg gtgacgttct gttccgaaat cacctccgcc cacccgatag
    10081 atgtgaagtt ttccagcgcg gcagtttcca ccgtgtaggc gttgcctggc tgtatcgtca
    10141 cgttctggat gggctgattg tttgcaatcg cgatgttcca cggttgagtg gtgcggctgt
    10201 caggcccatc cgaacggcgg aagaccagac taacggtcgc agggacctta tccgcgttca
    10261 ctaaagtaat agaggtctta aatgcgccgc catcggcaat ctgaggaatg gtgcggcgtg
    10321 tgctaatggc tgtaactgtg aactccaccg ccagccgttc ttcaagggca ggtgaaccgg
    10381 cgatgctggc gccggaaacc gtcccaaagt agacgtccgg catcaaaccg gtagcgtccg
    10441 ccgcgaccgt aaacaggaac ggggcttctc cacttgccgg aagagttgca actttcaacc
    10501 atgcgccaat atccgcattt gccgccacag ccacggtggt tccacccgct aggtctctca
    10561 ggcgcagatt cgctgtggcc acctcgttga ctttggccgt gaggcgtaac ggacccggcg
    10621 ggtcgaaact caacaccgtg ccgtgaaccg agaaggtcgt gggcagcgat atagtctgta
    10681 tctggccgcc ggattcccct gcattctgga cgcgtatcgt gacgctgccc gtataggtac
    10741 ccgcccgcaa cccgttgggg ttcgcaataa cagccaggtc gatctgctgg cccactgcaa
    10801 tcgaacccgt ccttggactc gtggacagga agggtacatt ggtggacacg gaaaacgcag
    10861 cgggcaacga gccgttgttg gttagagtaa cgggaaaaga tacgggggtc gttgatccgg
    10921 cgttagtatc catctccacg gtggacgggc tcggtgtcac attgggcggc ggcatggtga
    10981 tggtgaaaag cctggtattg gaaaacgtgc tcgtcccgcc cgtttcgcat agtatgtccg
    11041 tccggttgtg cacacgaatg ctcgccgcac ggggcgtgcc cagcagcact ccgggaacgc
    11101 tggcgatcag ccgtgtacca ctgacgaata tggtgggaag gtcactgcca tctatttgca
    11161 ccacacttgc gcgcgggcaa gcgacaccgc agacggttaa gcagatggag ggtccccgga
    11221 atccggtgcc atccacctgg agcgagactc ccaaaatgct cgccggcacc gaacccggtg
    11281 tcaggctgga aatctgtagc cccggaggaa gttgggcctt cgcggtggca atagcgaaaa
    11341 acagcaacag taccactgaa ccaaagccgc gtcggaacat aatcagccct ctccgtgtga
    11401 gtgataggaa gtctagcaca cggtatttca ttggtggaat gcgagccgta ctcctgaaaa
    11461 gtaatagcag tactaaagcc cggagcagct ccccgccgcc tcgcggcact cgttcgtgaa
    11521 cgggaaccgc gtgtcccggc gaagaatcgt caggacgcgc gtgcgcaccc ggttgcggtc
    11581 gagttccatg atgcgggcgg tgccatcggt gcgggccacc gcaagccgct tcgcatcgaa
    11641 actgaaagcc atggcgcgca cgggtgccgc atcgctcgaa atcgtgaaga gttcgttacc
    11701 acccgtcgcg tcccacagga cgatggaatt accgtcggtc tgcgtggcca ggcgacttcc
    11761 gtcctccgcc accgcggcta atcgcaccag accggtaggc ggcgaaaaga cgcgctcctc
    11821 actcttcgtt tcgagatccc accgccgcgc cttgcccgac cggctggctg tcatgagcca
    11881 tctgctgtcc gcgggcagag ccaggaaaga aaacgtgccg tccggatctt tctgttccca
    11941 tatcggcgcc ggcgagccgg tgtcccacac cgcaaatacg ccgttcgagc cgcccgccgc
    12001 aaagcggcgc ccgtcgccgc tgaatgtgac ggcagagact ctctcagccg ggatccgata
    12061 ttgttcgagc ccggctcccg tcgcggacca cagccgtatt tctcccgctt ccgctccggt
    12121 aaggattcgc gccccatccg gcgagatggc tatggacgta atgccgctct gcttcaacaa
    12181 gaccggaggg agcagagcgc ccgtgtcagg attgaaccct cccacgatgc cccggagcgt
    12241 tcccgccgcc agaacattcg cattgctgaa acgcacagcc gctacggggc cgtcgaggcg
    12301 aagccgctcc tcgtttgaaa gccgccgcaa ttcgctgtac gtctggtctc cgtgcgcgac
    12361 atcggcatcc cacaacgcca cggttccgtc cgccgcgccg gtggccagat agttgccgtc
    12421 gggactgaac gaaatcgcga tggcctcgtg cccttcgatt tccgccgcgg tggccgcctc
    12481 caggctcgtg tacaacgcgc tgagagtctc gggcccatcg cggcgcaagt tcgcctgata
    12541 ggccatcagg agcgccagct tgccatcgga tgcgcgcagc tctttggctt tcgccagaag
    12601 ttttccggtt acctgcgccc ggcttataaa aagccagatg acaagcacca gcgcgatgct
    12661 cgccgcgatt tgtccagcga tgaggagata gcggcgccgt ttcgccgcct gtgccgcgcg
    12721 ggcctctgca tccaccgctt cccggctgga acgcagaaag gctgcctccg tttccgtgaa
    12781 atcctccgcc cgttccgcaa accacccttc cgcttctttt agaagcgcgc cgcggagcag
    12841 gcagtccgga tcgcgaccga gccgcaacca ttccgacaaa ccgacgccaa acgtgcggcg
    12901 ccagtttaaa aacgcgcggt cttcctccag ccactgcgag tagcgcttcc aggcgcgaaa
    12961 caactcttcg tgcgccacct ccacggtagg gccggctgcc tgcccggacg acaccgtcaa
    13021 cagccgcgct tttggaccgg ccagaatgcc cacaacctcc tcgatattct tcacctgctc
    13081 cggtgccgcc gatgccggat ggagttggga gatcgccgtc cgaaccctgg tgtacctgcc
    13141 gtccggctgc gcctcggcga gccgcagaaa aacgcggcgc gagatttcag gctggctatt
    13201 ggaaagccac tccgtgaaca cattctccgc ctgagtgtgc aacgcgcccg aaagcccgcc
    13261 cattccgcga tatgcgtcaa gagacaggcg gcccgagacg gcgcattctt cccacaaccg
    13321 catcagcgtg tactgaagca gcggaagagc gcccgcctgg ccggacgcct cgcgcaccag
    13381 aacgtccgcc aggcccggct ccatgtccac gttggcgatg cgggccggtt cttcgatcgc
    13441 cgcgcgcagt tcgccttcgg ccatgccacc caccagaaca tgacggctct cgagcgccga
    13501 agcgaaaccc acaaatccgg cgcattcacc gtagaaatcg gcacgcagcg tcaacagcac
    13561 gacagacggc ccgccggccg acgcggcata cagcaggttt ttgatgaaag cctctcgtgc
    13621 tttctcatcg cggcaaacgg tgaagacctc ttcaaattga tcgacgagca gtgccatacg
    13681 cgttccggcg ggctgattca acagggccgc cgcaatcgac aaatgcagcc gccgctcatc
    13741 gtgcaggaaa ccatcgatca tctctgccag agccttgccg gtagtttccg cgtgcgcatg
    13801 ccgcaacagt tcgatagaga gcgattcgag cgggttcgcc gcgggcttga aggcgatcac
    13861 cggccatatt ttgccgcccg gcaaagcacc tttctgaatc tggtacagaa cgccggcgcg
    13921 cgcgagggaa gatttcccgc tgccggaagc tcccaggatg
//
LOCUS       DASTMK010000376         6113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_178600, whole genome shotgun sequence.
ACCESSION   DASTMK010000376 DASTMK010000000
VERSION     DASTMK010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178600"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFQ79_16420"
     CDS             complement(<1..467)
                     /locus_tag="VFQ79_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141309.1"
                     /translation="MNLQALRKNLLPSEAEQDEGFRQEILRLSHQSLKIIAAVQAGAA
                     VAITLAHFAFGRGLGTVGVRLLQSGIMIALGGFLLAMHRLPAAYARSRTIAVVTAFAS
                     GSVATWFSLWAAPRAPYMDNFIPSQVTLVVLVAIAAIPLKPKHTLALCLSLAG"
     gene            complement(510..2759)
                     /locus_tag="VFQ79_16425"
     CDS             complement(510..2759)
                     /locus_tag="VFQ79_16425"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0141310.1"
                     /translation="MTPERFQQVDRIFQDAVECGEAGREAFLAKSCAGDAELEAEVRS
                     LLASSAEAAGRIGDALQEAAQTFPYGDTHGEVGSMAGPYLLVRQIGSGGMGTVYQAIR
                     SDGEFFQTVAVKLVHRGMDTRSILQRFRTERQILANLTHPNITVLLDGGTSSEGRPYL
                     VMEYIEGELLLDWCERNRLTIRQRIELFRQICSAVQFAHERKVIHRDLKPGNVMVTPA
                     GLPKLLDFGIAKLLMEELLPDGTAVTETHFRFMTPDYASPEQVRGEPITEASDIYSLG
                     VMLYELLTGSRPFALTGRSPSEIETAICTGTAPPPSHAVRDPKLSHELAGDLDNIAGM
                     AMRKEPERRYRSAMELSDDLARYLEGRPVKARKDSISYRASKWLRRRRSSVAIAALLI
                     AATAAALPNFFRRPPVSLEAISLCDQARELTRMDARIRRQQGGIPPNLREAASLYEKA
                     TRLDPSYVPAWIGLAETIEFAIDFDQPRWKELASQSLKAAQECVRLDPKNAKAWELIG
                     NIHNREWNFSAAANAFKRALELNALAPYALRNYTTWLGYTGRTDEALELVRQALSHPI
                     AMSNPDPHVISGRSRAILLSHQGALLFRAGRYQEAIESNRQALEIEATYRIANWITGL
                     ALEALGDFTGAEKEFRLALAVAPEDTRPLAALGHLLARIPQRRGEALEIEKQLLDLHD
                     RGRPVLCHAAIVRTGLGNIPGALDLLERAAGNQEGPLPSILVDQRMEPLRPEPRFQAL
                     VDRVQPYRR"
     gene            complement(2756..3364)
                     /locus_tag="VFQ79_16430"
     CDS             complement(2756..3364)
                     /locus_tag="VFQ79_16430"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU0141311.1"
                     /translation="MRATINDNRMGEPQTITALLKRHDQGDAQALGEMLPLVYSQLRR
                     MAGALMRSARSDQTLQPTALVHEAFLKMSAGDEFTASGKAHFLALAARVMRQVLVDHA
                     RSRASAKRGGGKAFATLDEAMVYSAERAPALLALDLALDALGKLDERKARMIEMRYFG
                     GLTGEEIACVMCVGTATVTRELRMAEAWLGRELGRPEAASGR"
     gene            3436..>6113
                     /locus_tag="VFQ79_16435"
     CDS             3436..>6113
                     /locus_tag="VFQ79_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141312.1"
                     /translation="MIIGAPIRGIVSGLAYIRDTITINDAFLSELNSQIAEKKLPANG
                     TATITIAAREIKHAPNYILRLTNYKVVVIADEYDAAGGSIEVSGTAGANGAKGPNGEK
                     GYASATPQNNKDGKPGGPGGSGQPGSKGMPLQLFCKRLKRANLLSRGGSGGNGGAGGN
                     GGEGGSAKIIVVDPGGGKFQEYVGTKGGNGGRGGNGAAGGSGGQVTVRYVERLAGSPS
                     VSVTGGAGGVGGAGGTGGKGGYQSANGVAGAKGSNAAKGPDGQASVQQLEESVFWDQV
                     RTLIDGTRSYMWAEYRTRMGEYYLRCFAPGKADTAKFLSLAANEFRSILWLRPQEPKA
                     QVMLGNIAANLIPLGTARDFYLTPDFPRYEQAFTDYAPMVQNMVDSAKDLLMHANNVQ
                     YNRTKLSTQIAHIQSSGLIIESERAEAAVEKLQAEGVVSGYDGRINALEAEIDAKRHE
                     LAQARLELGGAFISSVLEAIGAVAAVVGAVYTGGASLAALPGLLVAAQDSWGKLSTDA
                     NGQVKYQDTYLTDWVEWKDGKPVLKQEIKDLGAGIEQVVNSVKPAVNATLDLIDKAKV
                     LGDIGQSTVDGELQSELKELLRRKAELEFERAQAKLGVKQAELRLATIDLKAQQAQAD
                     LAAAQQMLDTLTADNKLLGRVTAMLMRRAQDYMSHLLQFQFWASRALDIYTMKDHTGL
                     LPFDIGYVHPDVEEDAYTAFGRGSSSHALNLMEAYLTSWARLPGIIILRNEYESYRMD
                     LLTQTQFWSFSGSHPVLQEFRQKGSVEFELGLSDIYQDRFECKIEGATVALAGAVSAE
                     PLINCILMQRGESASVRLDGSVVHFNYPPRGCPLLAVKAAAQINSAPPSTPEAFWGRS
                     PAAKWRLYLEPDAMTKVDLSGVTEILLCFSYKAFIPP"
BASE COUNT         1258 a         1848 c         1879 g         1128 t
ORIGIN      
        1 aagcccgcca gcgacaggca gagcgccagc gtatgcttgg gcttcagtgg aatcgccgcg
       61 atggccacca gaactaccag cgtgacctgg ctcggtatga aattgtccat gtacggcgcc
      121 cggggtgcgg cccaaagcga gaaccacgtc gccacactgc ccgaggcaaa cgccgtcacc
      181 accgcgatcg tccgcgaccg ggcataggct gccggaagac ggtgcatcgc cagcaggaag
      241 cctcccagcg cgatcatgat gccgctttgc agcagccgta ctcccaccgt tcccaggccc
      301 cgcccgaacg cgaaatgcgc cagcgtgata gccacggccg caccggcttg aacggccgcg
      361 atgatcttca aagactggtg gctgaggcgc aggatctcct ggcggaatcc ttcatcctgt
      421 tccgcttcgc tggggagaag gtttttccgg agcgcctgta ggttcattaa gaggcttctt
      481 gagattactg taccgcgtcc ttccccgcct taacgccggt aaggctgtac tcgatcaaca
      541 agcgcctgaa atcgcggctc gggtcgcagc ggttccattc gctggtccac caggatggat
      601 ggcagcggcc cttcctgatt gcccgcagct ctttcgagca gatcgagagc tccgggtatg
      661 tttccaagtc ccgtcctcac gatagcggcg tgacacaaga cgggccgtcc acggtcatga
      721 aggtcgagca gttgtttctc aatctccagc gcctcgccgc gccgctgagg aatgcgggcg
      781 agcagatggc ctaaagccgc cagcggcctg gtatcctcgg gtgcgacggc caacgccagg
      841 cgaaactcct tctcagcgcc ggtaaagtcg ccgagagctt ccagggcgag tcccgtgatc
      901 cagtttgcga tccggtacgt agcctcaatc tccaaagcct ggcgattgct ttcgattgcc
      961 tcctggtaac gccccgcacg aaacagtaac gccccttgat gagataaaag gatcgcccgc
     1021 gaacggccgc tgatcacgtg aggatcggga ttcgacatcg caataggatg ggaaagcgcc
     1081 tgccggacca gttccagagc ttcatcggtg cggccggtgt aaccaagcca tgttgtgtaa
     1141 ttacgcagtg catacggagc cagagcattc agttccaaag cacgtttgaa agcatttgcc
     1201 gcggcggaga agttccactc ccggttatga atgtttccga tgagctccca agccttggca
     1261 tttttgggat cgagacggac acattcctgt gcggccttca aactttgcga ggccagttct
     1321 ttccatcgag gctggtcgaa gtcgattgcg aactcgatgg tctccgccag accgatccag
     1381 gcaggtacgt agctggggtc cagccgggta gccttttcat aaagtgaggc tgcctcccgc
     1441 agattcggag gaatgccccc ctgctggcgc cgtattcgag catccatgcg cgtcagttcg
     1501 cgagcctgat cgcaaaggga aatcgcctcc aacgaaaccg gcgggcgccg gaagaagttc
     1561 ggaagcgcag cggctgttgc cgcaatcagt agcgccgcaa tggccacaga cgagcggcgc
     1621 cggcgaagcc acttggaagc tcgataggag atgctgtcct tgcgcgcctt caccggccgg
     1681 ccctccagat agcgggcgag atcgtctgag agttccatag cgctccggta gcggcgctcc
     1741 ggttccttac gcatcgccat gccggcgata ttgtcgaggt ccccggccag ctcatgcgaa
     1801 agctttggat cgcgaacggc atggctcggc ggaggcgctg taccggtgca gatggccgtt
     1861 tcaatctcgg aaggagaacg tccggtcagt gcaaaaggcc gcgagcccgt cagcagctca
     1921 tacagcatga cgcccagcga gtaaatatcg gatgcttccg tgatgggttc gccgcgcacc
     1981 tgctcgggac tcgcgtagtc cggcgtcatg aaccggaagt gcgtttccgt aacggctgtg
     2041 ccatccggca ggagttcctc catcagcagc tttgcgattc cgaaatccaa tagttttgga
     2101 agaccggccg gtgtaaccat cacgttcccc ggtttgagat cgcgatgaat caccttgcgc
     2161 tcgtgagcaa actgaacggc actgcagatc tggcggaaga gctcgatccg ctggcgaatc
     2221 gtaagccggt tgcgctcaca ccagtccaac agcagttccc cttcgatgta ctccatcacc
     2281 aggtagggac ggccttcgct ggatgtgccc ccatcaagca gcaccgtgat attcggatgg
     2341 gtaaggttgg ccagaatctg ccgctccgtg cggaagcgct ggagaataga ccgcgtgtcc
     2401 atgccccggt gcaccagttt cacggctacg gtttggaaga attccccgtc ggatcgaatg
     2461 gcctggtaga ccgttcccat gcctccgctg ccgatctgcc gcaccagcag gtagggccct
     2521 gccatcgacc ccacttcacc atgagtgtcg ccgtacggaa aggtttgcgc ggcttcctgg
     2581 agagcatcgc cgattctccc ggcggcttcg gcggagctgg cgagcagaga gcgcacctcc
     2641 gcctcgagtt cggcatcgcc ggcacaggac ttcgccagaa aggcttcccg gccggcctcg
     2701 ccgcactcca cggcgtcctg gaagatgcgg tcgacctgtt gaaaccgttc cggcgtcacc
     2761 gccccgacgc agcctcgggg cggcctaatt cgcgccccag ccaagcctcc gccatgcgca
     2821 gctcacgggt tacggttgcc gttcctacgc acattacaca ggcgatttcc tcgcccgtga
     2881 gtccgccaaa ataacgcatc tcgatcatgc gagcttttct ttcgtccagt ttgccgaggg
     2941 cgtccagcgc aagatccagc gccagaagcg cgggtgcgcg ctctgccgag tagaccatgg
     3001 cttcatccaa ggtagcgaac gcttttccac cgccgcgttt ggccgacgcc cgggaccggg
     3061 cgtggtctac cagaacctgc cgcatcactc gcgcggccag ggccagaaag tgcgccttgc
     3121 cgctcgcggt aaactcgtcg ccggcggaca ttttcaggaa cgcttcgtgc accagggcgg
     3181 tcggctgcaa ggtttgatcg ctgcgggcgc ttcgcatgag tgctcccgcc atccgccgaa
     3241 gctgactgta gaccaaaggc agcatctcac ccagggcctg cgcatccccc tggtcgtgcc
     3301 gcttcagcag cgctgtgatt gtctgtggct cgcccatccg gttgtcattt atcgtagcgc
     3361 gcatgatcaa atccggtgcg cgattccgta tccgttgcag aagttgacac acagcaacaa
     3421 ccaaggagca cggaaatgat cattggagcg ccgattagag gcatcgtttc aggtctggcc
     3481 tacatccggg acaccatcac catcaatgat gcgttcctca gcgagttgaa cagccagatt
     3541 gccgaaaaaa aacttcccgc gaacggcacc gccaccatta cgattgctgc tcgcgaaatc
     3601 aaacacgcac ctaactatat actgcggctc accaactaca aagtcgttgt gattgcggac
     3661 gaatacgacg ccgccggcgg ctcgattgag gtgagcggca ctgccggagc aaacggtgcg
     3721 aaagggccga atggagaaaa gggttatgcc agcgccacgc cccagaacaa caaggatgga
     3781 aaaccgggag gacccggcgg cagcggtcag cctggcagca agggaatgcc gttgcaactt
     3841 ttttgtaaac gcctgaaacg ggccaatctg ctgtcgcgcg gcggcagcgg cggcaatgga
     3901 ggagccggtg gaaacggcgg agagggaggc agcgccaaaa tcatcgtagt cgacccgggc
     3961 ggcggcaagt tccaggagta cgttggaacc aagggcggca atgggggccg cggcgggaat
     4021 ggcgccgccg gcggcagcgg cggccaagtc accgtccggt acgtcgaacg gctggcagga
     4081 agcccctctg tttccgttac cggaggtgcg ggcggcgttg gcggagccgg gggaaccggc
     4141 ggcaagggcg gataccagtc ggcaaacggg gtggctggtg ccaaaggatc gaacgcggcg
     4201 aaaggtccgg acggccaggc cagcgtacaa cagttggaag agagcgtgtt ctgggatcag
     4261 gtcagaacct tgatcgatgg cacccgttcc tacatgtggg ccgaataccg cacacgcatg
     4321 ggcgagtact atctgcggtg ctttgctccg ggcaaggccg atacagcgaa gtttctcagc
     4381 ttggccgcaa acgagtttcg cagcatactt tggttgcgcc cccaggaacc caaggctcag
     4441 gtgatgctgg gcaacatcgc tgcgaacctc attccgctcg gcaccgctcg cgacttttat
     4501 ctgacgccgg actttccgcg ttacgagcag gcattcacgg actatgcgcc gatggttcaa
     4561 aacatggtcg acagcgcaaa ggacttgttg atgcacgcca acaacgttca gtacaaccgc
     4621 accaagcttt caactcagat cgcccatatt caatcaagcg ggctgatcat cgaaagcgaa
     4681 cgcgccgaag cggccgttga aaagctgcaa gcggaaggag tggtttcggg ctacgacggg
     4741 cgcatcaacg cgctcgaagc ggagatcgac gcgaagcggc atgagctggc gcaagctcgc
     4801 ctggagcttg ggggcgcctt tatttcctct gtgctggagg cgatcggcgc tgtcgcggct
     4861 gtggtcggag cggtctacac cggcggcgcc tcgctggcgg cgttgcccgg ccttctggtt
     4921 gctgctcagg acagttgggg caagctcagc accgatgcga atggccaggt caagtaccag
     4981 gatacgtatc tgaccgactg ggtggagtgg aaggacggca aaccggtgct caagcaggaa
     5041 atcaaagatc tgggcgccgg catagagcag gtagtgaaca gcgtcaagcc cgccgtaaat
     5101 gccaccctgg atctcatcga caaggccaag gtgcttggcg acatcggcca gtccaccgtg
     5161 gacggtgaac tccagagcga gttgaaggag ttgctgcgcc ggaaagccga gctcgagttc
     5221 gagcgcgctc aggccaagct tggcgtgaag caggcggagt tgcgcctggc caccatcgac
     5281 ttaaaggcac agcaggcgca ggccgacctg gcggcggcac agcagatgct ggacaccctt
     5341 acggcggaca acaagctgtt gggacgggtt acggcaatgc tgatgcggcg tgcccaggat
     5401 tatatgagtc atctgcttca gttccagttc tgggccagcc gcgccttgga catctatacg
     5461 atgaaggacc atacgggttt gctcccgttc gacatcggtt acgttcatcc cgatgtggaa
     5521 gaggatgctt acacggcctt cggccgcggg agcagcagcc atgcactgaa tctgatggaa
     5581 gcctatctca cttcgtgggc ccggcttcca ggcatcatca ttctgcggaa cgaatacgaa
     5641 tcgtaccgga tggatctgct tacgcaaacg cagttctgga gcttcagtgg cagccatccc
     5701 gtgcttcagg agttccggca gaaaggcagc gtcgagtttg aactcggcct gagcgacata
     5761 tatcaggacc ggtttgaatg caagatcgag ggagcaacgg tggcgctcgc cggcgccgtc
     5821 tcagccgagc cgctgattaa ctgcatcctg atgcagcggg gcgagtcggc atcggtccgg
     5881 ctggacggca gtgtggtcca cttcaactac ccgccacgcg ggtgtcccct gcttgcggta
     5941 aaagcggcgg cgcagatcaa cagtgccccg cccagcacac cggaagcgtt ctggggccgc
     6001 agcccggcgg caaaatggag gctctacctg gaaccggatg ccatgacaaa agtggacctc
     6061 tcgggtgtca ccgagattct cctctgcttc agctacaagg cattcattcc acc
//
LOCUS       DASTMK010000377         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_561292, whole genome shotgun sequence.
ACCESSION   DASTMK010000377 DASTMK010000000
VERSION     DASTMK010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561292"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFQ79_16440"
     CDS             <1..754
                     /locus_tag="VFQ79_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0141313.1"
                     /translation="QHMFYRRLGSASEDVIEAMYRRMDELVGKTMAFADNGTALFVLS
                     DHGFCSFRRGVNLNTWLYRNGYLALRDGAQESGPFFKNVDWSRTRAYALGLSGLYLNL
                     EGREASGIVRPGAEADALARELIEGLEALRDSNEVAAVRKVYRTAALYRGPYLDAAPD
                     LIVGYAEGYRVAWDAATGKTGVEVIEDNDKAWSGDHCVDPLLVPGVLFSNVPIDATDP
                     GIEDMAPTALRLFGIPVPAWMEGAPVLTDAVS"
     gene            768..959
                     /locus_tag="VFQ79_16445"
     CDS             768..959
                     /locus_tag="VFQ79_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141314.1"
                     /translation="MFGPAIKVNKELWERIRTCAAAAGYSSPEEFVRHILEREVAKLE
                     DAQSDEEIARKLKGLGYLE"
     gene            956..1711
                     /locus_tag="VFQ79_16450"
     CDS             956..1711
                     /locus_tag="VFQ79_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141315.1"
                     /translation="MIPDAAAALLLALAGTVCGVGTLLAMRRFSDQRAVRSAKARVRA
                     HLYEMRLFGDDPVLALRAQKKLLLWNLRYARLALLPAAIVAVPAILIMSQLEAVYGSE
                     PLMPGETTLVTVQLKRGTQPVAADLTAPSGFTVETPPVRIADLRQVCWRIRARGSTDG
                     ELHFQTAGESLHRAIHSGPGLRYVTPECSSAPLASIWYGCALESNSVESIAVDYRTRK
                     ISLFGFDAHWLVWFALFWLAAMLLLRRRFGVTF"
     gene            complement(1815..>2701)
                     /locus_tag="VFQ79_16455"
     CDS             complement(1815..>2701)
                     /locus_tag="VFQ79_16455"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141316.1"
                     /translation="KNVIERYAAEARELEAHLNAAAGSSAHAPEKVQTAMVDQATLRQ
                     LRSLGYSAGSSEREFTLTGQGIDPKDRTEVLRLLYLAVSPEAGTPVSRRAELLRRALA
                     ADPGNPTVYLHLGDEYGRTGRREDALKLYQDGIRNGVRTAWLYSRLGFLYMAQGNLEQ
                     AITAYERAAQLNPADCDSLNDLGMTYLDTGRPGDAERVFQWCLATGEDHALSHNGLGL
                     VAVRKDDFAAARGHFEKAVQMDPGLLEGYLNLGRIYKMSGDYAKARQCFETFLAKASP
                     AQYAQIIPRLREELRQMR"
BASE COUNT          470 a          829 c          870 g          532 t
ORIGIN      
        1 ccagcacatg ttctaccggc ggcttggttc ggcttcggaa gacgtaatcg aagcgatgta
       61 ccggcgcatg gacgagcttg tgggtaaaac gatggccttt gccgataacg gcacggcctt
      121 gttcgtcctt tccgatcacg gcttctgctc gttccggcgc ggcgttaacc tcaacacatg
      181 gctgtaccgc aacggatacc ttgcgctgcg cgacggggcg caggagagcg gcccgttttt
      241 caaaaatgtc gactggagcc gcaccagggc ctacgccctg ggactgtccg gcctgtatct
      301 caatctcgaa ggccgcgagg cgagcggcat tgtccgcccc ggcgccgaag cggacgcgct
      361 cgcgcgtgaa ctcatcgagg ggctggaggc gctgcgcgac agcaatgaag tggcggctgt
      421 gcgaaaggta tatcgcacag ccgctctcta ccgcggtccg tatctcgatg ccgctcctga
      481 cctgattgtg gggtatgcgg agggttaccg tgtcgcctgg gatgcggcga ccggaaaaac
      541 aggtgtagag gtgatcgagg acaacgataa ggcctggagc ggcgatcact gcgtggaccc
      601 gctgctggtt cccggcgtgt tgttttcgaa cgtaccgatc gatgcaaccg accccggcat
      661 cgaggatatg gcgccgaccg cgctccggct gttcgggatt ccggtgcccg cctggatgga
      721 aggcgcgccc gtgttgaccg acgcggtatc ctgatcggga caggattatg ttcgggccgg
      781 ccatcaaagt aaataaggaa ctctgggagc gcatcaggac gtgcgctgcc gcggcgggat
      841 atagctcgcc ggaggagttt gtccgccaca ttctggagcg cgaagtcgcc aagctggagg
      901 atgcacagtc cgacgaagag atcgcacgca agctgaaggg gctgggctac ctcgagtgat
      961 tccggatgcc gccgcggcgc tgctgctggc tctggccgga acggtatgcg gtgtgggcac
     1021 gctgctggcg atgcggcgct tctccgacca gcgcgccgtg cgttccgcca aggcacgcgt
     1081 tcgcgcacat ctttacgaga tgcgcctctt cggggacgat ccggtattgg ctctgcgcgc
     1141 gcaaaagaag ctgctgctgt ggaacctgcg gtatgcccgg ctcgcgctgt tgccggcggc
     1201 gattgttgcc gtccccgcga ttctgatcat gagccaactg gaagcggtgt atggaagcga
     1261 gccgctgatg ccgggggaga cgacccttgt taccgtgcaa ctgaaacgcg gcacgcaacc
     1321 cgtggcggcg gatctcaccg caccgtccgg cttcaccgtg gagacacctc cggtgcgtat
     1381 cgcagacctc cggcaggtct gctggcgcat tcgcgcacgc gggagcaccg atggtgagct
     1441 ccacttccaa accgctggag aaagcttgca tcgtgcgatc cactcaggcc ccggccttcg
     1501 ttacgtgacg ccggagtgtt cgtccgctcc cctggcgtcg atctggtacg gatgcgccct
     1561 cgagagcaat tcagtggagt ccatcgccgt ggattaccgc acccgcaaga tctccctctt
     1621 cggtttcgac gcgcactggt tagtctggtt cgccctcttc tggcttgccg ccatgctgct
     1681 gctccggcgg cgtttcggcg tgacgttctg agttgacgga agtccgaaaa aggccacttg
     1741 ctgacgcgcg tggctcagta ggatcattgc gtttcggagc cgcgaccgcg agggagcggt
     1801 gtctaccacg gctgctatcg catctgccgc agctcttccc gcaaccgggg gatgatctgc
     1861 gcgtactggg cgggcgaagc cttcgccaga aacgtttcga aacactggcg cgccttcgcg
     1921 tagtcgccgg acattttgta aatgcgcccc agattcaggt agccttcgag gagaccggga
     1981 tccatttgga ccgctttctc gaaatgcccg cgagccgccg caaaatcgtc ttttcgcacc
     2041 gcaaccagcc ccaggccgtt atgggataaa gcatgatctt cgcctgtcgc caggcaccat
     2101 tggaagaccc gttcggcgtc tccaggtctc ccggtatcca ggtaagtcat gccgaggtcg
     2161 ttcaggctgt cgcaatcggc aggattgagt tgggctgccc gttcataggc ggtgatcgcc
     2221 tgctccaggt ttccttgcgc catgtacaga aaaccgagcc gcgagtaaag ccacgccgtc
     2281 cggactccgt tgcgaatgcc atcctggtat agcttcagcg cgtcttcgcg gcggccggtc
     2341 cggccatact catcgcccag gtgtaagtaa acggttggat ttcccggatc tgccgccagt
     2401 gcccgccgca gcagttcggc gcggcgcgag acaggagtgc cggcttccgg agaaacggca
     2461 agatacagca ggcggagaac ctcggtgcga tctttcgggt cgatgccctg gccggtgagc
     2521 gtgaactcgc gctcggaaga cccggccgaa tagcccagag accgcaattg ccgcagcgtg
     2581 gcctgatcca ccatcgccgt ctgtaccttc tcgggagcgt gggccgaact gccggcggct
     2641 gcattgaggt gggcttccaa ctcgcgtgcc tccgccgcat aacgctcgat cacgtttttg
     2701 g
//
LOCUS       DASTMK010000378         8511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_81971, whole genome shotgun sequence.
ACCESSION   DASTMK010000378 DASTMK010000000
VERSION     DASTMK010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81971"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="mraY"
                     /locus_tag="VFQ79_16460"
     CDS             complement(<1..1091)
                     /gene="mraY"
                     /locus_tag="VFQ79_16460"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU0141317.1"
                     /translation="MLYWLLYEQLFPYFSPFRIFQYATIRVAFASLTALFLCVVLGPW
                     LISKLREFEIGQHIREEGPKSHQKKAGTPTMGGVLIVISIVVPTMLWTNLSNQYVWIA
                     LFGLLSFAAIGFYDDYTKIRRMRNLGLSGKQKFGLQVLVALVVGFWLLVLHSYGMYST
                     EINMPFFKQFKPDLLIDEFLANPWTYPLAFIFFFLFMVVVLVGSSNAVNLTDGLDGLA
                     IGLMIIAAGAMTVLTYVSGHREFATYLEIARLPGASELAVFCGAMTGASIGFLWYNAH
                     PAEIFMGDVGSLALGGGLALIAVLIKQEILLIFIGGVFVLEALSVIIQVGSFKIRGKR
                     VFKMAPLHHHFEALGWQESKIIARFWILGL"
     gene            complement(1098..2435)
                     /gene="murF"
                     /locus_tag="VFQ79_16465"
     CDS             complement(1098..2435)
                     /gene="murF"
                     /locus_tag="VFQ79_16465"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU0141318.1"
                     /translation="MKFSLRQIADVLQWEKPVPDLAVAGWSIDSRTIKAGDLYFALKG
                     ARHDGNAFLEDAFANGAAAAVAQVPLEGKPVIVVPETLASLQELARWARREWDGKVVG
                     ITGSAGKTTTKDVIAAMLAVERNTGKTAGNLNNHIGLPLSILRLPDEADVAVLEFGMN
                     HAGEIRDLARIAKPDIGVVTNVGYAHIEAFDCLDAVAAAKRELIESLPPDGVAVLNAD
                     DPRVRNFGEVHPGRTVTFGLSQDAAVRAEEIEYSAEGVRFRALGCRFETAMAGRHAVL
                     NILAGLAVAGVFGIAPERLQDAVRGLRAATMRSERIQKDGITVWNDCYNSNPDAVRAM
                     LDVLKETPASRRIAVLGEMLELGRWSEALHREVGRYVAGSGISVLVGIRGAARYMVDE
                     AVRAGLEAGAAYFFEDPSEAGDFVRSMAAEGDAILFKGSRSVQVEKALERFFG"
     gene            complement(2432..3910)
                     /locus_tag="VFQ79_16470"
     CDS             complement(2432..3910)
                     /locus_tag="VFQ79_16470"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU0141319.1"
                     /translation="MTLGEIFEGVELKAGLPEQLANTPVAGLEYDSRRIQPGFLFFAF
                     EGARADGRKYARQALDKGAVAVVGESPPPADFQGAWIEVAHGRQALATIARNFYGKPD
                     ERLFLTGITGTNGKTTTSYLIDSVLRAAGKTTALIGTIEYHLADEILPAVNTTPESLD
                     LFRLFAELERRGGTHVTMEVSSHALALGRSFGLRFHTGVFTNLSRDHLDFHKTMEDYF
                     AAKRRLFTGYGAPPPPFAVLNSDDEHGRGMEVPAGTHVFRYGLDADREIRASNIVSGF
                     TGLRFDVVSPEGSFEIQSPLVGRFNVYNILAACCTGMSYGFTPETIARGIAECGPVPG
                     RFERIDEGQRFLVVVDYAHTDDALRNVIGVARSLSPRRVITVFGCGGDRDRTKRPLMG
                     MAAGELSDFVVLTSDNPRSEDPLSIINDALVGLGRYDTPHVVEPDRMKAIHRAIQEAG
                     ESDIVIIAGKGHEPYQVLKDRTIDFDDREAARSVLQKLGYRK"
     gene            complement(3915..6023)
                     /locus_tag="VFQ79_16475"
     CDS             complement(3915..6023)
                     /locus_tag="VFQ79_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU0141320.1"
                     /translation="MKIRDWATWRRKSRGGVYDPPSWGTSDETAEPESVRRLRHVARF
                     ALIWAIIIIVRLVYLQIFSHAEYSHLAEQQQERMLEILAPRGTIYDRSGEPLAMSVPV
                     DSVCINPQRIKDFAVAAEILSGVLNIDKQQLLERMRWAKEHDRGFLWVKRKLEPEESR
                     RLRALNLGWVEFRKESRRFYPKGQLASHVIGAVDHRERGNAGLELSLDDELAGRAGAL
                     RMMADVRQRGFETEVSVEPVPGVSLTTTIDERIQFTAEKALAEAVVSNQCTTGSLVVM
                     DPRNGDILALANYPTFDPNAPVADLENRANLSVSTPFEPGSVFKVITVSAALETTRLR
                     PETIIPCGNGSINLFGRLIRDHKAHSALPMEEVIAKSSNIGAINIALQVGNERLLEYV
                     RRFGFGSRTGVPLPGESSGLVRDLRLWGKSSIGSVAMGHEISTTTVQLAQACSAIANG
                     GLLIKPRLILQRQAPGMKAEPQPPHPAKRVLKPETSITMRRMMEGVVLHGTGTRAKLD
                     GYTAGGKTGSAQIFDLVHRVYTHKYNASFIGFAPLTNPAIVVVVTLNGSSKFGGAVAA
                     PVFREVASTALRILDVPRDLPYELPQLAKKEPEDDYDLAIAEVGGAPDAEMLLATTGN
                     VREGNGSMPVLVPAAYPVELLGPAVPDFSGKTVRKVIEESAAQGLPVEFTGSGVARGQ
                     YPPAGVRLKSGERVRVRFAR"
     gene            complement(6057..6548)
                     /locus_tag="VFQ79_16480"
     CDS             complement(6057..6548)
                     /locus_tag="VFQ79_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141321.1"
                     /translation="MASLATFYNRFAGSKTMDSAFAAGRSRAHDDVFRSRAIPNEDIY
                     FFVKQIDNTNVVRQPDSQAPQTCWKLIGTASILSLLLVALLIPSAYGLLAGYEMETLR
                     EQREMLEREKLSLELEASRLLSPERLQELANAQQFIDPAPGTVVYLDPKDGSLALNVP
                     AKK"
     gene            complement(6556..7416)
                     /gene="rsmH"
                     /locus_tag="VFQ79_16485"
     CDS             complement(6556..7416)
                     /gene="rsmH"
                     /locus_tag="VFQ79_16485"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU0141322.1"
                     /translation="MHAPVLVDEVIEYLALRSGGIYLDATAGLGGHTAAIARRVTEGM
                     VIANDRDAESLDMARRNTAEWASRIRYVQGTFSGLSSTLPVLGLAQVDGLVADLGVSR
                     YQLTDAGRGFSLMARGPLDMRMDRSQGVPAADIVNHYAEKALADLIYQLGEERRARKI
                     ARAIVRARPIRDSLHLAGVIEEAVHRTGRLHPATQTFMALRIFVNDETGQLDDLLEQA
                     PSLVKSGGRIVIISFMSLEDRKVKRKFQALARAGRARILTRHVVTPKEEEVRRNPASR
                     SAKLRALEMS"
     gene            complement(7426..7911)
                     /locus_tag="VFQ79_16490"
     CDS             complement(7426..7911)
                     /locus_tag="VFQ79_16490"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141323.1"
                     /translation="MDQNPTPADVVEPPRGFLPARVDEKGRLKLPVAVQQYLASFGQE
                     KVFITSLDTRIARIYPISVWKENEIFFEQASEDPDAAEDVAFIAAYLGADSELDGQGR
                     LLLPTDLRRTLNLENQQVWLSCYKGRINVFSKEIFEQRKQRALGGLDEKLRLLEKRGF
                     R"
     gene            8114..>8511
                     /locus_tag="VFQ79_16495"
     CDS             8114..>8511
                     /locus_tag="VFQ79_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEU0141324.1"
                     /translation="MPSLYDLDPGPECPELVRAIIEIPKNSANKYEYDGKLELFRLDR
                     SLYSPMHYPGDYGFIPGTVAEDGDPMDVLVLVQEPSFTGCMIEVRPVGILNMVDNQEG
                     DQKVLAVPNRNPRYDQIHTMDQIFPHVRRE"
BASE COUNT         1731 a         2728 c         2352 g         1700 t
ORIGIN      
        1 agcccgagaa tccagaaccg ggcgataatc ttcgactcct gccatccgag cgcttcgaaa
       61 tgatgatgca ggggagccat cttgaagaca cgtttgccgc gtattttgaa gctgcccacc
      121 tggatgatga ccgacagcgc ctccagcaca aatacgccgc caatgaagat cagcaggatt
      181 tcctgcttga tcaacaccgc gatcagcgcc agcccccctc cgagggcgag cgaccccaca
      241 tcgcccatga agatttcggc cgggtgcgcg ttgtaccaga gaaagccgat ggatgcgcct
      301 gtcatggccc cgcagaacac ggccagctcc gaggcacccg gcaacctcgc gatctccaga
      361 taggtggcga attcgcgatg cccgctcaca taggtgagca ccgtcatggc gcccgcggcg
      421 atgatcatca atccgatcgc cagaccatcc agcccgtccg tgagattcac cgcgttcgac
      481 gaaccaacca ggaccactac catgaacaga aagaagaaaa tgaatgccag cggataggtc
      541 cacgggttgg cgagaaactc gtcgatgaga agatcgggct tgaactgctt gaagaacggc
      601 atattgattt cggtggaata cataccgtag gaatgcagca ctagcagcca gaaaccaacg
      661 accagcgcca ccaggacctg caacccgaac ttctgcttcc cgctgagccc aaggttgcgc
      721 atcctgcgta ttttcgtgta gtcgtcgtag aacccgatgg ccgcgaagct taacaaccca
      781 aacagcgcga tccacacgta ttggttcgac aggttggtcc acagcatggt gggaaccaca
      841 atcgagatga caatcaggac accccccatt gttggcgttc cggccttctt ctgatgggac
      901 ttgggacctt cttcccgaat gtgctgcccg atttcaaatt ccctcagctt cgagatcagc
      961 cacggtccca gcaccacgca cagaaacagg gccgtaaggc tcgcgaaggc tacacggatg
     1021 gtggcgtact ggaaaatgcg gaacgggctg aagtaaggaa agagctgttc atataggagc
     1081 cagtaaagca tctggtgcta cccgaaaaac ctctctaagg ctttctccac ctgaacactc
     1141 cgggatcctt taaaaaggat ggcatccccc tccgcggcca tacttcggac gaagtcgccc
     1201 gcctcgctgg gatcctcaaa aaagtacgcg gcgccggcct ccagaccagc acggactgct
     1261 tcgtcgacca tataacgggc cgcgccgcgt atcccaacaa gcacagatat accgctgccc
     1321 gcgacgtaac ggcctacctc gcgatgcagc gcctcagacc agcgtccaag ctcgagcatt
     1381 tctccaagca cggcgatgcg gcggctcgcg ggcgtctctt tcaagacgtc aagcatggcg
     1441 cgcaccgcgt cggggttgga gttgtagcaa tcgttccaga ccgtgatgcc gtccttttgt
     1501 atgcgctcac tgcgcatcgt cgcggcccgt aagccgcgga cggcgtcctg aagccgctcc
     1561 ggcgcaatgc cgaatacacc ggctacggcc aggccggcaa gaatattcaa taccgcgtga
     1621 cgtccggcca ttgccgtctc aaaccggcaa cccagcgcgc ggaaccgtac tccttccgcg
     1681 gagtactcga tctcttcggc gcgaacggcc gcgtcctgcg acaacccgaa ggtcacggta
     1741 cgtcccggat gcacttcgcc gaaattccgc acgcgggggt cgtcggcatt cagcacggcg
     1801 acaccatcgg gcggaagcga ttcgatcagt tcccgcttgg ccgctgcgac tgcatccaag
     1861 cagtcgaaag cctcgatatg ggcgtagccg acgttggtaa ccacaccgat atccggtttc
     1921 gcgattcgcg caagatccct gatttcaccg gcatgattca tcccgaattc gagtaccgca
     1981 acatcggctt catcgggcaa gcgcagaatc gagagcggca gccctatgtg gttgttcaag
     2041 ttcccggctg tcttgccggt gttcctctcc accgcaagca tcgccgcaat aacgtccttc
     2101 gtcgttgttt tcccggcact gcccgtgatg ccaaccacct tgccgtccca ttcgcggcgc
     2161 gcccaccgcg cgagttcctg aagcgacgcc agcgtctccg gaacgacgat aacgggtttt
     2221 ccttcgagcg gtacctgggc gacagcggcg gccgctccgt tggcgaacgc atcctctaga
     2281 aaagcgttgc cgtcgtgcct cgcgcctttg agtgcaaagt acagatctcc cgctttgatc
     2341 gtgcgcgaat cgatactcca acccgccacg gcgagatccg gcacaggctt ctcccactgg
     2401 agcacatccg cgatctgccg cagcgaaaac ttcatttacg gtaacccaac ttctgtagaa
     2461 cggagcgcgc cgcctctctg tcgtcaaaat cgatggtgcg gtccttcaga acctggtagg
     2521 gttcatgccc cttgccggca ataataacga tgtcggattc gcccgcctcc tgaatcgccc
     2581 ggtggatcgc tttcatgcga tccggttcca ccacgtgcgg agtatcgtag cggcccaggc
     2641 caaccagcgc gtcgttgatg atcgacagcg gatcctcgga gcgcggattg tccgatgtca
     2701 gcaccacgaa gtcgctcagt tctcccgccg ccattcccat caggggccgc tttgtccggt
     2761 cgcggtcgcc gccacagcca aacaccgtaa tcacacggcg gggcgagagg ctgcgggcaa
     2821 cgccgatcac atttcgcagc gcatcatccg tgtgcgcgta gtcgacgacg acaagaaaac
     2881 gctgcccctc gtctatgcgc tcgaaacgtc cgggcaccgg accgcattcc gcaatgccgc
     2941 gagcgattgt ctccggagtg aaaccgtaac tcataccagt gcagcacgcc gccagaatgt
     3001 tatagacgtt gaaccggccc accagcggcg attggatctc gaaggaacct tccgggctca
     3061 ccacatcgaa cctcaacccg gtgaatccgc tgacgatatt cgacgcccgt atctcccgat
     3121 ccgcatcaag gccgtaccgg aaaacatggg ttcccgcagg aacctccata ccgcgtccgt
     3181 gctcgtcgtc ggaattcagc accgcgaatg gcggaggagg agcgccatag ccggtaaaaa
     3241 gcctccgctt ggcggcaaaa tagtcctcca tggtcttatg gaaatcgagg tgatccctgc
     3301 tcagattcgt gaatactccc gtatggaacc gcagcccgaa cgagcgcccc agcgcgagtg
     3361 cgtgggaaga cacctccatt gtgacatggg tccctcctct gcgctccagt tcggcgaaga
     3421 ggcgaaagag gtccagcgat tccggcgtcg tgttgacagc gggcagaatc tcatcggcaa
     3481 gatgatactc gatggtgccg atcagtgcgg ttgtcttgcc tgccgcgcgc agcacggaat
     3541 cgatcaggta ggaagtagtg gtcttgccgt ttgtacccgt gatgccggta agaaacaacc
     3601 tctcatcggg cttgccgtag aagtttcttg cgatggtagc gagcgcctgc cgtccatgcg
     3661 ccacctcgat ccatgcgcct tgaaaatccg ccggcggcgg cgactctcca acaacggcaa
     3721 cggcgccctt gtcgagcgcc tgccgagcat acttgcggcc gtccgcccgg gctccctcga
     3781 acgcaaaaaa caggaatccc ggctggatgc gtcgcgagtc atactcgaga ccggcgacgg
     3841 gggtattcgc cagttgctcc ggaagtccgg cttttagttc gacaccttcg aagatctcac
     3901 ccagtgtcat ggtttcaccg ggcgaaccgc actcggactc tctctcccga cttgagccgg
     3961 acacctgccg gcgggtattg gcctctggct acgccgctgc cggtgaactc taccggcagc
     4021 ccctgggcgg ctgattcctc aatcactttt cgaactgtct tgcccgaaaa atccggcacg
     4081 gccgggccga gcaattccac cgggtaagcc gcgggcacca gcaccggcat tgaaccgttc
     4141 ccctcccgca cgtttccggt ggtggcgagc agcatctccg catccggtgc gccgcctact
     4201 tccgcaatcg caagatcgta atcgtcctcg ggctcttttt tggccagttg cggcagttcg
     4261 tagggcaggt cgcgcggaac atccagaatt cgaagggccg tcgatgcgac ttcgcggaat
     4321 accggagcgg ctaccgctcc tccaaatttc gatgagccgt tcagcgtcac aacgacaacg
     4381 atggcaggat tcgtcagcgg tgcaaatcct ataaacgacg cgttgtactt atgcgtatac
     4441 acccgatgaa ccaggtcgaa gatctgcgcc gaaccggttt tgcctcccgc cgtgtagccg
     4501 tccagtttcg cgcgcgtacc ggtgccgtgc aaaacgacgc cttccatcat ccgccgcatg
     4561 gtgatagagg tctccggttt caggacgcgc ttggcggggt gcggcggctg cggctccgct
     4621 ttcatgccgg gcgcctggcg ctgcaggatc aggcgcggct ttatcaacag tccaccattg
     4681 gcgatcgcag agcacgcctg cgccagttgc actgtcgtgg tgctgatctc gtgtcccatg
     4741 gccaccgatc cgatggagct tttgccccac agccgcagat ccctgaccag gcctgaagac
     4801 tcgcctggca ggggaacgcc ggtgcgcgaa ccgaatccga atcgccggac gtattccaac
     4861 aggcgttcat tcccgacctg gagagcaatg ttaatcgctc cgatgttact cgatttcgcg
     4921 ataacctctt ccatcggcag agcggaatga gccttatggt cgcgaatcag gcggccaaac
     4981 agattgatgg atccgttgcc gcacggaata atggtttccg gcctcagccg tgtagtctcg
     5041 agggcggcgg ataccgtgat caccttgaac acggagcccg gctcgaacgg cgtcgacaca
     5101 gaaaggttgg cgcgattttc caggtccgcg accggagcgt tcgggtcgaa ggtcggatag
     5161 ttggcgagag ccaggatgtc tccgttgcgt gggtccatta ccaccaggct gcccgtcgta
     5221 cactggttcg aaaccacggc ctcggcgagc gccttttccg cggtgaattg aatcctttcg
     5281 tcgatcgtgg tcgtgagact cacgcccggc acgggttcga ccgacacctc cgtctcgaat
     5341 cccctttgcc ggacgtccgc catcatgcgc aacgcaccgg cccggcccgc aagctcatcg
     5401 tccaggctca actcgaggcc ggcattgccg cgttcccgat ggtccacggc gccgataacg
     5461 tgtgaggcca attgtccttt cggatagaac cggcggctct ctttgcggaa ctcgacccag
     5521 ccaagattca gcgcgcgcag ccggcgcgat tcctccggtt ccagcttccg cttcacccac
     5581 aggaagccgc gatcatgctc tttcgcccag cgcatccgtt ccagcaactg ttgcttatcg
     5641 atgttcagga cgcccgaaag aatctccgcg gccaccgcga aatcttttat ccgttgggga
     5701 ttgatgcaga ccgaatccac cggcacgctc atggcaagcg gctcgccgga acggtcgtag
     5761 atggtgccgc gcggcgccag gatctccagc atgcgctcct gctgctgctc ggcaagatgg
     5821 ctgtattcgg cgtgggagaa gatttgcaga taaacgagcc ggacgatgat gataatcgcc
     5881 cagatcaggg caaaccgggc cacatgacgc agccgccgga ccgattccgg ctcggcggtc
     5941 tcatcgcttg ttccccagct tggtgggtcg tacacaccac cccggctctt ccgccgccag
     6001 gtggcccagt ctcgtatctt catatcgcgc acgcacggtc gagtccgcga ggggcgtcac
     6061 ttcttggccg gtacgttcag ggccagggaa ccgtctttcg gatccaggta caccaccgtt
     6121 cccggggcgg gatcgatgaa ttgctgggca ttcgccagtt cttgaagccg ctccggactc
     6181 agcagccgcg atgcttccag ctccaggctg agtttctcgc gttccagcat ttcccgctgt
     6241 tcccgcagcg tctccatctc atagcccgcg agcaggccgt acgcgctcgg gatcagtaac
     6301 gcaacgagca gcagagaaag gatagacgct gtgccgatca gcttccagca ggtttgcggc
     6361 gcctggctgt cgggctgccg tacgacgttt gtgttgtcaa tctgcttcac gaaaaagtag
     6421 atgtcttcgt ttgggattgc gcgggaccgg aacacatcgt cgtgtgcccg gctcctgccg
     6481 gcagcaaacg cgctatccat cgttttggat cccgcaaatc ggttgtagaa agtcgccagt
     6541 gacgccattt tctgatcagc tcatctccag cgcccgaagc ttagcgctcc gcgacgccgg
     6601 gttccttctc acttcttctt cctttggggt gaccacatgc ctggtcagaa ttcgcgctct
     6661 gcccgcacgg gccagcgcct gaaacttccg cttcaccttc ctgtcctcca gcgacatgaa
     6721 agagatgatt acaatcctgc cgccgctctt caccagcgat ggagcctgct caagaagatc
     6781 gtccaactgc ccggtctcgt cattcacgaa aatccggagg gccataaacg tctgcgtagc
     6841 cgggtgaagc cgtcccgtcc ggtgcacggc ctcttcgatg actcctgcca ggtgcaggct
     6901 gtcccgaatt ggccgcgccc ggacgattgc tctggctatc ttccgcgccc tcctttcttc
     6961 gcccagttga taaatcaaat cggcgagcgc cttttcggca tagtgattga caatgtcggc
     7021 agccggcacg ccctggctgc gatccatccg catatccaac ggccctctgg ccatcagcga
     7081 aaaccctcgt ccggcgtccg tcaactgata ccggctcaca cccaggtcgg cgaccaagcc
     7141 gtcgacctgg gcgagtccca gaaccggcag ggtggaggac agacccgaaa acgttccctg
     7201 tacgtagcgg atgcggctgg cccattcggc cgtattccgt ctggccatgt cgagcgactc
     7261 cgcgtcccgg tcgttcgcaa tcaccatccc ttcggtgacc ctgcgagcaa tcgccgcggt
     7321 gtgccctccg aggccggctg tcgcgtccaa atagataccg ccgctccgga gcgcgagata
     7381 ctcgatcact tcgtcgacca gaaccggagc gtgcatacat gacgcctacc tgaaaccccg
     7441 cttctccagc aggcgcagtt tctcgtccag cccgccgagc gcccgctgct tccgctgctc
     7501 gaaaatctct ttactgaaaa cgttgattct gcccttgtaa caggacagcc aaacctgctg
     7561 gttctcgagg ttcaacgtac gccgcaagtc cgtgggcagc aacagacgcc cttgcccgtc
     7621 cagctctgaa tccgcaccca ggtacgcagc gatgaaagcc acatcctcgg ccgcgtccgg
     7681 atcttcactt gcctgctcga agaaaatctc gttttctttc caaaccgaaa tagggtaaat
     7741 tcgagctatg cgagtatcca gactggtaat aaaaaccttt tcctgtccga aactcgctaa
     7801 atattgctgt acggctacgg ggagcttgag gcggcctttt tcatctacgc gtgccggcag
     7861 gaacccccgg ggaggctcga cgacatcagc gggcgtggga ttttggtcca cctagttcca
     7921 ctcgtaacca ctttttacca ctgaatggat tgtcgcatgg cctgccgaag gttgcaatac
     7981 ttttttccca ttactacctt aaggtctaca tgttgtagca agacgatacg agccgcaatt
     8041 gctcgtatca gggacgacga acaccgttcc gtaatggcac gttgaaagcg cacctgcatg
     8101 ctacgctgta caggtgccat cgctttacga tctggatccc ggtcccgagt gccccgagct
     8161 ggttcgggcg atcattgaaa tcccgaagaa ctctgcgaac aagtatgaat acgacggcaa
     8221 gctggagcta ttccggctcg accgttcttt gtactctcct atgcactacc ccggcgatta
     8281 cggcttcatc cctggaacgg tagccgaaga cggggatccc atggatgtcc tggttctggt
     8341 gcaggaaccc agctttaccg ggtgcatgat cgaagtacgc ccggtgggca ttttgaatat
     8401 ggtggacaac caggaaggag accaaaaggt gctggccgta ccgaaccgga atccccgcta
     8461 cgaccagatc cacacaatgg atcaaatttt cccgcatgtg cgccgggaaa t
//
LOCUS       DASTMK010000379         9677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_307090, whole genome shotgun sequence.
ACCESSION   DASTMK010000379 DASTMK010000000
VERSION     DASTMK010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307090"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFQ79_16500"
     CDS             complement(<1..230)
                     /locus_tag="VFQ79_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU0141325.1"
                     /translation="MTHLRKMMLEELQRRNYSQTTVNAYLKIVGSFANYFQRSPNQLG
                     PAEIRAYQVYLLKERKLGARTVGHHTAALRFF"
     gene            complement(467..1609)
                     /locus_tag="VFQ79_16505"
     CDS             complement(467..1609)
                     /locus_tag="VFQ79_16505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0141326.1"
                     /translation="MKLGKKFADLKVRPKLIVLHNIFFLVLTCAVYFALIPLFERTGT
                     DPDVVHGARLVLFLVLGIVYVLAVILLEAVIMPRYVYQPLQLMLQADEATRQGDREHE
                     YISDSGIPGDEIGQIMRSRNETVRQLRRHEDELEAALVRLEKAKQSLLEQDRLASLGL
                     MSASVAHELNTPIAVLQGSIEKLMETIPNAQAQERLSRMLRVARRLHRISETLLDFAS
                     ARTDDMGSVSIRPLVDEAWALLAIDEKASEVSFRNDVDSRHAVIGNADRLIQVFVNLL
                     RNSLYAVHSAGHLTVWSREVEEDSATWIEMSFEDDGPGIPADLLPNIFEAFVSSRLDS
                     RGTGLGLTVAEGIVTRHGGRIRAENREGGGAMLVVLLPSASTATTP"
     gene            1745..2392
                     /locus_tag="VFQ79_16510"
     CDS             1745..2392
                     /locus_tag="VFQ79_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU0141327.1"
                     /translation="MSAIFPKSADRYLRLAAVSLAAVGASVIGVYAYLTQPRVIDTGY
                     SPVQPVAYSHKLHAGNLGMDCLYCHSTVEKSSFAAIPTTETCMNCHVRVKPQSPLLVK
                     VRESHATGQPIEWVKVHKLPDYVYFNHQAHVTSGVSCVSCHGRVDQMIEVKQVQPLSM
                     AWCLDCHRSPAAHIRPPSLVTKLDWTPERDAAEIGREIIAQKGIVPPTNCSGCHR"
     gene            2389..5292
                     /locus_tag="VFQ79_16515"
     CDS             2389..5292
                     /locus_tag="VFQ79_16515"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEU0141328.1"
                     /translation="MSRKGKNYWRSLDQLADKPEFRNWLHREFPENASELLESGSRRT
                     LLKLMAASFGLAGLAACRRPVEKILPAAKGVEGYIPGNPLYYSTAMSLGAGVSGLLVE
                     THDGRPTKVEGNPRHPANAGTASAYVQASILGLYDPDRSQTVLKNGRKSSWEEFAQFA
                     GSHFTGDGSGLRILSEQVTSPSLDAVRRHLLKRFPKAQWIEYEPLSQDEAMEGARLAF
                     GDYARAHYQFEKADVVLSLDADILGLDSPSTVYTRDFAKRRRPESMNRLYAGEAQYSV
                     TGTMADHRLRLCASDIGRLALELAEALKLNPAALTVLPNSNDARAKWIQAVARDLVAH
                     RGRSLVVVGPRQPAAVHALAHLMNAALGNAGGTVTYSRSEWQPQISAMRQLAGEMASG
                     AVQTLVILGGNPVFNAPADLQFEAALRKVPTVIHHGLEYDETAALAGWHVPAAHYMES
                     WSDARAWDGTATVQQPLIQPLYGGKTTAEVTALLSGYKDQRAYDIVRNYWVAQWPAAQ
                     SEKIWRRSLHDGVVAGTRSPEWKTAINAKRVLDASAALESTGSGLEVAFHQSASLFDG
                     RFANNGWLQEAPDPLTKIVWDNAALIGPATAGKLGLSTGDMVTLEKEGRRITVPVSIQ
                     PGQADDSISLALGYGRSRCGRVGTGVGVNVYPLRASAGLHMATGVTLRKTGGKYRLTT
                     TQDHHSMEGRDIVRESALEDFRKNPHGHSEGAAHPELFSLYKEHSYEQGNQWGMVLDL
                     NACTGCNACLVACQAENNIPIVGKDQVERGREMHWIRLDRYFDGSEEDPQVVYHPVAC
                     QQCENAPCESVCPVAATTHSPEGLNDMAYNRCIGTRYCANNCPYKVRRFNFLEFQKDV
                     AAVQKMAYNPDVTVRVRGVMEKCTYCVQRIQEKKILAKSEGRRPIRDGEIQTACQQTC
                     PAQAITFGNLRDPESKVAKLAGDPRNYAVLSELNIKPRTTYLTKLRNLNPELA"
     gene            5295..6674
                     /gene="nrfD"
                     /locus_tag="VFQ79_16520"
     CDS             5295..6674
                     /gene="nrfD"
                     /locus_tag="VFQ79_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU0141329.1"
                     /translation="MADLALRRPLSLELDPPMVTGDLTYSEVTQKVSEITESGTPRAW
                     YVAISITGGITLLLGAMLGYLALTGIGVWGNNSPIGWAWDITNFVFWIGIGHAGTLIS
                     AILFLFRQKWRTSINRFAEAMTLFAVACAGIFPLFHVGRPWRAYWLFPIPNTDLNMWQ
                     NFRSPLLWDVFAVSTYATVSLLFWYVGLIPDLATLRERAKSLFRKRIFGVLSLGWRGS
                     SHHWRHYEMAYLILAGLSTPLVLSVHTIVSFDFAVSVIPGWHTTIFPPYFVAGAIFSG
                     FAMVVTLMVLARQIFGLQNLVTMKHLENMNKIMLATGMIVGYAYATELFTAWYSGNKY
                     EQFAFSNRAFGPYAWAYWTMVTCNVIAPQVFWFKRFRTSIPMMFAVSILINIGMWFER
                     FVIIVTSLHRDYLPGSWGYFSPTIVDILTFLGSFGLFLTLFLLFVRFLPMIAISEVKG
                     VMAQQRARH"
     gene            6709..7293
                     /locus_tag="VFQ79_16525"
     CDS             6709..7293
                     /locus_tag="VFQ79_16525"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEU0141330.1"
                     /translation="MIHSEDSVLDQPVSTTAAQVEIHGILGEFDSPEALLEAVKAMRE
                     RGYTRLDAYTPFPVHGMDKALGARRSRLGLVSLTGGVLGASAALLLQWWTGAVDYPLV
                     IGGKPFFAFEFSIPVTFELTVLFAAFGTVLGMLAFNGLPRLYHPAFNYSRFRGATDDR
                     FLLAVDAADPKFDSEKTLMLMRSLGAAHPEVVEA"
     gene            7290..7865
                     /locus_tag="VFQ79_16530"
     CDS             7290..7865
                     /locus_tag="VFQ79_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0141331.1"
                     /translation="MKLLATSAAFFAMTILWGCSGSTSRKPPIEIFADMDRQGKPRPQ
                     QAAYRPVPGTVAVGQLNEDDAYHTGIENGFYVAKNPLPMTRETLETGRRRYDIYCAPC
                     HDRTGSGHGIVSQRSGSWLPTNLKDDRVRNMVDGEIFSVATMGRRSMPAHRFQVSAAD
                     RWAIVAYVRALHRASAGSIEDVPRELRSDLR"
     gene            7870..9078
                     /locus_tag="VFQ79_16535"
     CDS             7870..9078
                     /locus_tag="VFQ79_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141332.1"
                     /translation="MPAHTTQFERLESYEIAPQGWTHARNSIALLTAVGVAASAAAYA
                     ADAPRFFASWLVSFAFFVSIGLGALFFVMVQHLTGAAWSVTLRRLMENCAGTLPASIL
                     LFVPVVFGFNDLYKWSNAAAVSPDSILLGKSAYLNPSFFLLRSALYFAIWGLWAWKLR
                     AISAKQDRTGSIETVRDGARWSAPGVILLILTGTLASFDWIMSLDPHWYSTIFGIYFL
                     SGGALAFIAALILIALAFRRAGILRYAITVEHYHDLGKWLFALTIFWAYIAFSQYLLI
                     WYANIPEETIWFRHRLEGSWTWMSAALLIGHFMVPFLVLLPSSSKRNLKILGLMSAWI
                     LAAHFIDLYWQIMPNFHAHRVEFHWADAATFAASGGIVSWVFWTGTRARPAVPVGDPR
                     LEKSLEFENA"
     gene            9087..9503
                     /locus_tag="VFQ79_16540"
     CDS             9087..9503
                     /locus_tag="VFQ79_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141333.1"
                     /translation="MQDHKQHGGYDYGEPRSGLIALFGIAAIVLVVAAVFAIQTYYDH
                     VREKHLYEKVLAPVSDDLQSLRAKEDGELHSYGYTDRDAGKVRIPIERAMELLEKEAA
                     AGKLAYPTRPAPVKVAAQAEAQPQEGEAPHAPASLD"
     gene            9481..>9677
                     /locus_tag="VFQ79_16545"
     CDS             9481..>9677
                     /locus_tag="VFQ79_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141334.1"
                     /translation="MRLPAWISLLFIAALLPAADPQLPAELEGVGVVERIGDSIDLGL
                     TFTDETGRAVALREYFSSDRPV"
BASE COUNT         1885 a         2847 c         2913 g         2032 t
ORIGIN      
        1 aaaaagaaac gcagtgcggc cgtgtggtgt ccgacggttc gcgccccaag ttttctttcc
       61 ttgagcaggt agacctgata ggcgcggatc tccgctggtc cgagctgatt cggagaacgt
      121 tggaaatagt tcgcaaacga ccctacgatc ttcaggtagg cgtttacggt agtctgggag
      181 taatttcgac gctggagctc ttccagcatc atttttcgaa gatgggtcac agggacctcc
      241 ttgtgaccca cacactacgt caaccactga tggaaatgcc gaacctactg acacgcgtag
      301 cgtccgccgc gctggcggct tcgttctgac ggcgtagcat ttacgatccg tcccttggcc
      361 agacgcctga acatcatgtt ccagaatagg gctcagcgac taggagcaag gactttcggg
      421 ataaggcact gccagtttat gtttccctgt cctgccaaat tatcgttcaa ggtgtggtgg
      481 cggtactggc cgatggcagc aggaccacga gcatcgcgcc gccgccttcg cgattctcgg
      541 cgcggattct tccgccatgc cgcgtcacga ttccttcggc caccgtcagg cccaacccgg
      601 tgccgcgcga atccaggcgg gatgaaacga atgcttcgaa aatgttcgga agaagatcgg
      661 cgggaatgcc gggaccgtcg tcttcaaaac tcatctcgat ccaggttgcg ctatcctcct
      721 ccacttcgcg ggaccacacc gtcaggtggc ccgccgaatg gacggcatag agggaattgc
      781 ggagcagatt gacaaacacc tggatcaagc ggtcggcatt cccgatgacg gcgtggcgcg
      841 agtccacgtc gttgcggaaa ctgacctcgg acgctttctc atcgatagcc agcagggccc
      901 acgcctcgtc caccaggggg cgtatggaaa cgctgcccat gtcatccgtt cgcgcgctcg
      961 caaaatccag cagcgtctcg ctgatccggt gaagtctcct ggcaactcgc aacattcgcg
     1021 aaagccgctc ttgcgcctgt gcgttgggga tggtttccat cagcttttcg atggagcctt
     1081 gcagcactgc gatgggcgta ttcagttcgt gggcgacgct ggcgctcatc aggccgaggc
     1141 tcgccagccg gtcctgttcg agcaagcttt gcttggcctt ttccagccgg accagcgccg
     1201 cctccaactc atcttcgtgc ctccgcagtt gtcttacggt ttcgttgcgc gagcgcataa
     1261 tctgcccgat ctcatccccg ggtatgccgc tgtcgctgat atattcgtgt tcccggtcgc
     1321 cctgccgcgt ggcttcgtcg gcctgtagca tgagttgcag cggttggtac acgtatcgcg
     1381 gcatgatgac cgcttccagc agaatcacag ccagtacgta cacgatgccg agcacaagaa
     1441 ataacaccag tctggctccg tgcaccacgt cgggatccgt accggtcctc tcaaaaagcg
     1501 ggatcagtgc gaaatagacg gcacacgtca gcaccaggaa gaaaatgttg tgcagaacga
     1561 tcagtttcgg cctgaccttc aaatccgcaa acttttttcc cagtttcatt gaatcttttc
     1621 ccctcgccgg atcatcactg caattgctgt aaaacaagaa attttattgt gattgagcat
     1681 tgaatgcggg tttggtagaa aatatctata gcaagagcaa tctgggcctg ggttcccgaa
     1741 ctggatgagt gccatattcc ctaagtcagc cgacagatat ctacgcctgg ctgccgtctc
     1801 gctggcagca gttggcgcct ccgtgattgg agtctacgct tatcttactc agcctcgcgt
     1861 aatcgacact ggctattctc ccgtacagcc ggtagcctac agccacaaac tgcacgccgg
     1921 caatctcggc atggattgcc tctactgcca ttcgacggtg gagaaatctt cgttcgccgc
     1981 cattcccacg acagagacct gcatgaactg ccacgtgcgc gtgaagccgc agagcccctt
     2041 gctcgtgaaa gtgcgtgaaa gccacgccac gggccagccg atcgagtggg taaaggttca
     2101 caagctgccc gactatgtgt acttcaacca ccaggcacat gtcactagcg gtgtcagttg
     2161 cgtcagttgc catggccgtg tggaccagat gattgaagtg aaacaggtac agccgctcag
     2221 tatggcgtgg tgcctggatt gtcatcgtag tcccgcggcg catatccgtc ctccatcgct
     2281 ggtgacgaag ctggactgga cgccggagcg cgatgcggcc gagatcgggc gggaaatcat
     2341 tgcgcaaaag ggaatcgttc ctcccaccaa ctgttcgggg tgccaccgat gagccgcaaa
     2401 ggaaagaatt actggcgcag tctggaccaa ctcgcggaca agcccgagtt tcgcaactgg
     2461 ctgcatcggg agtttccgga aaacgcctcg gaactgctcg agagtggttc acggcgcacc
     2521 ctgctgaaat tgatggcggc gtcgttcggc ctagccggcc tggccgcttg ccgccggccg
     2581 gtggagaaga tcctccccgc ggcgaaaggc gttgagggct acatccccgg aaatccgctc
     2641 tactacagca cagcgatgtc tttgggtgcg ggtgtgagcg gcctgcttgt ggaaacacac
     2701 gacggcaggc cgacgaaagt ggaagggaat ccgcggcatc cggcgaatgc aggtacggct
     2761 tccgcttacg tacaggcttc cattcttggg ttatacgatc cggaccgctc gcagacagtg
     2821 ctcaagaacg gacgcaagtc gagctgggag gagtttgcgc agttcgccgg ttcgcatttc
     2881 acgggagacg gatccggatt gcgcatattg agcgagcagg tcacatcgcc gtcgctggat
     2941 gcggtgagac ggcatctgct gaagcgtttt cccaaagccc aatggatcga gtatgagccg
     3001 ctttcgcagg acgaagcgat ggaaggcgcg cggctggcct ttggcgatta tgcgcgcgcg
     3061 cactatcaat tcgagaaagc cgatgtagtg ctgtcgctcg atgcagacat ccttggtctc
     3121 gattcgccct ccacggtgta cacgcgggac ttcgcgaagc gccgccgtcc ggagtcgatg
     3181 aaccggctct acgccggcga agcacaatac tccgtcacgg gcacgatggc ggaccaccgg
     3241 ctccggctgt gcgcgtcgga tatcgggcgg cttgcgctgg agttggcgga agccctgaag
     3301 ctgaatcctg ccgcgcttac ggttcttccg aactcgaacg acgcccgcgc gaaatggatt
     3361 caagcggtgg ctcgcgacct ggtggcccat cgcggacgtt ctcttgtggt ggtggggccg
     3421 cgccaacctg ccgcggtgca cgcgctggcg cacttgatga acgccgccct cgggaatgcg
     3481 ggggggaccg taacgtattc ccgctcggaa tggcagccgc agatttcggc gatgcggcaa
     3541 ctggccggcg aaatggcgtc gggggcggtt cagacgctgg tgatcctcgg cgggaatccc
     3601 gtgtttaacg cgcccgccga tctccagttc gaagccgcgc tccggaaggt acccaccgtt
     3661 atccatcatg gtctcgagta cgacgagacg gccgctctcg cgggctggca cgtacccgcg
     3721 gcgcactaca tggaatcctg gagcgacgcg cgcgcgtggg acggaacggc tacggttcaa
     3781 cagcctctga ttcaacccct gtatggcgga aagaccacgg cggaagtgac ggccctgctt
     3841 tccggctaca aggatcaacg ggcctatgac atcgtgcgga actactgggt ggcgcaatgg
     3901 cccgcagcac aaagtgaaaa gatctggcgc cggtcgctgc atgacggtgt ggtggcggga
     3961 acgcgcagcc ccgaatggaa aacggcgatt aacgcgaagc gcgtgctgga tgcctccgcc
     4021 gcactcgaat ccaccgggtc cggcctggaa gtagcgttcc accagagcgc atcgctgttt
     4081 gacggccgct tcgccaacaa cggctggctc caggaagcac ccgacccgct gacaaaaatc
     4141 gtttgggaca acgccgcgtt gatcggcccg gcaacggccg gcaaactggg cctttccacg
     4201 ggtgacatgg tgacgctcga aaaagagggg cgccgaatca ccgtgcctgt ttcgattcaa
     4261 ccgggacagg cggatgattc gatctcgctc gcgctcgggt acgggcgcag ccgctgcgga
     4321 cgcgtgggta cgggcgtcgg agtgaacgtg tacccgctgc gggccagcgc cggcttgcat
     4381 atggcgactg gcgtcacatt gcggaagacg ggcggaaaat accggctgac caccacgcag
     4441 gaccatcatt ccatggaagg ccgcgacatc gtccgtgaga gcgccttgga ggactttcgc
     4501 aaaaatccgc acgggcactc cgaaggcgct gcgcatccgg agctcttctc tttatacaaa
     4561 gagcacagct acgaacaagg caaccagtgg ggaatggtcc tcgacctcaa tgcctgcacc
     4621 ggctgcaatg cctgtctggt cgcctgccag gccgagaaca acattccgat cgtagggaag
     4681 gatcaagtcg agcgcgggcg cgagatgcat tggatccggc tggaccggta tttcgacggt
     4741 tcggaggaag atccgcaggt ggtctatcat ccggtggcct gccagcagtg cgagaacgcg
     4801 ccgtgcgagt cggtctgccc cgtagcggcg accacgcaca gccccgaggg cttgaacgac
     4861 atggcgtaca accgctgcat cgggacgcgg tattgcgcga ataactgtcc ttacaaggtg
     4921 cgccggttca actttcttga attccagaag gacgtcgcgg ccgtgcagaa gatggcctac
     4981 aatcccgacg tcaccgtacg cgtgcggggc gtcatggaga agtgcacgta ctgcgtgcag
     5041 cgcattcagg aaaagaagat tctggcgaag tcagaaggcc gccgcccgat ccgtgacggc
     5101 gaaatacaga ctgcgtgcca gcagacctgt ccggcgcaag ccatcacgtt cgggaacttg
     5161 cgggaccccg aaagtaaggt cgccaaactg gccggcgatc cgcgcaatta cgctgtcctt
     5221 tcggagttga acataaaacc gcgcaccact tatttgacga agttgcgaaa tctgaatccg
     5281 gagcttgcat aacgatggcc gacctggcgc ttcgaagacc cctttccctc gaactcgacc
     5341 cgccgatggt tacgggcgat cttacttact ccgaagtaac tcagaaggta agtgagatta
     5401 cagaaagcgg aacgccccgc gcctggtacg tcgccatctc catcacgggc ggcatcacgc
     5461 tgctgctggg cgctatgctc ggatacctgg cgctcaccgg tatcggtgta tggggcaata
     5521 actcgcccat cggctgggcc tgggacatca cgaatttcgt tttctggatc ggcatcgggc
     5581 atgccggaac gctgatctcg gcgattttgt ttttgttccg gcagaagtgg cgcacctcca
     5641 ttaaccgttt tgcggaagcg atgacgttgt tcgccgtggc ttgtgcgggc atctttccgt
     5701 tgtttcacgt tggccggccc tggcgcgcct actggctgtt cccgattcca aacaccgacc
     5761 tgaacatgtg gcagaacttc cgcagtccac tgctgtggga cgtcttcgcc gtcagcacct
     5821 acgcgacggt atcgctgctg ttctggtacg tcggattgat accggacctg gcaacgctgc
     5881 gcgaacgggc aaaatccctg ttccgcaaac gcatcttcgg tgtgctgagc ctcggctggc
     5941 gcgggtcgtc gcatcactgg cgccactatg aaatggcgta cctgattctg gcggggctct
     6001 ccactccgct ggtgctttca gtacatacga tcgtcagttt tgatttcgct gtctcggtga
     6061 ttcccggatg gcacaccacc atctttccgc cgtacttcgt cgcgggagcg attttctcgg
     6121 gctttgccat ggtggtgacg ctgatggtgc tcgcccgcca gattttcggc cttcagaatc
     6181 tggtgacgat gaagcacctg gagaacatga acaaaatcat gctcgccacg ggcatgatcg
     6241 tcggctacgc ttacgcgacc gaactcttca ccgcctggta cagcggaaac aaatacgagc
     6301 agttcgcgtt ctcaaaccgc gccttcggcc cttacgcctg ggcctactgg acgatggtga
     6361 cctgcaacgt tatcgctccg caggtgttct ggttcaagcg tttccgcacc agcattccga
     6421 tgatgttcgc agtttccata ttgatcaata tcggcatgtg gtttgagcgc ttcgtcatca
     6481 tcgtgacgtc gctgcatcgt gactacctgc cgggaagctg gggctatttc agcccgacga
     6541 ttgtggacat tctgactttc ctgggaagct tcggattgtt cctgacgttg ttcctgctgt
     6601 tcgtccgatt tttgccaatg atcgccatct cggaagtgaa aggcgtgatg gcgcaacaga
     6661 gagcgcgcca ctaatgacgg ttcacagcgc aacggagaga tttggaaaat gattcattcg
     6721 gaagattccg ttctcgatca accggtctcc accaccgccg ctcaagtgga gatccacggc
     6781 atcctcggcg aattcgacag cccggaagct ttgcttgaag ccgtgaaggc gatgcgcgag
     6841 cgcggctata cgcggctgga cgcttacact ccatttcccg ttcacggtat ggacaaggcg
     6901 ctgggggcgc gccgttcgcg gctggggctt gtcagcctga ccggcggcgt gttgggcgca
     6961 agcgcggcac tgctgcttca gtggtggacc ggcgcggtgg attacccgct ggtgattggg
     7021 ggcaagccat tcttcgcgtt tgaattctcg atacccgtca cgttcgagct gacggtgttg
     7081 tttgccgcct tcgggaccgt actgggtatg ctggccttca acggcctgcc gcgcctgtat
     7141 caccccgcgt tcaactactc gcgatttcgg ggagcgaccg atgaccgttt cctgctcgct
     7201 gtagacgccg ccgatccgaa attcgacagt gagaaaactc tgatgttgat gcgctcgctc
     7261 ggggccgctc acccggaggt agtggaagca tgaagcttct ggcaaccagc gccgcttttt
     7321 tcgccatgac gattctctgg ggctgctcag gctctacatc gcgcaagccg cccatcgaga
     7381 tcttcgcgga catggaccgg cagggcaagc cccggccgca gcaagccgca taccggcccg
     7441 ttccgggtac cgtcgcggtg ggccaactga acgaagacga tgcctatcac acggggatcg
     7501 aaaacggctt ctacgtggcg aaaaacccgc ttccgatgac gcgggaaacc ctggagaccg
     7561 gcaggcggcg atacgacatt tactgcgccc cgtgtcacga ccgcacggga agcggccatg
     7621 gcattgtctc gcagcgcagc ggctcctggc tgcccaccaa tttaaaggac gaccgcgtcc
     7681 gcaacatggt ggacggtgag attttttccg tggcgacgat gggccgccgg tccatgcccg
     7741 ctcaccggtt tcaggtttcg gcggcggacc gctgggccat cgttgcctat gtacgcgcgc
     7801 tgcatcgtgc ttcggcggga agcatcgaag acgtcccgcg cgaacttcga tcggacttga
     7861 ggtgatctga tgccggccca cacaacacaa ttcgaaagac tcgaatcgta cgagatcgcg
     7921 ccgcagggct ggacgcacgc gcgcaatagc atcgcattgc tcacggcggt gggcgtggct
     7981 gcaagcgccg cggcctatgc tgcggacgca ccgcgattct ttgcgtcgtg gctggtgtca
     8041 tttgcctttt tcgtaagtat cggcctcggt gcactcttct tcgtaatggt gcaacacctc
     8101 acgggagcag cctggagtgt gacgcttcgc cgcctgatgg aaaactgcgc aggcacactg
     8161 ccggcttcga tattgctgtt cgttccggtg gtgttcggct tcaacgatct gtacaaatgg
     8221 tcgaacgccg ctgccgtcag ccccgattcg attctactcg gcaagagcgc gtacctgaac
     8281 ccgtccttct tcctgcttcg atccgccctg tacttcgcta tttggggact gtgggcgtgg
     8341 aagcttcgcg cgatatcggc gaagcaggac cggacaggct ccatcgagac ggtccgcgat
     8401 ggcgcccgct ggagcgctcc cggggtaatc ctgttgatcc tcaccggaac actcgcttcg
     8461 ttcgattgga tcatgtcgct cgatccacac tggtactcca ccatcttcgg gatttatttt
     8521 ctttccggcg gcgcgctggc atttatcgcc gcgctgatac tgatcgcgtt ggcttttcgc
     8581 cgcgccggta ttttgcggta tgccatcacg gtcgaacact atcatgacct tggaaagtgg
     8641 ctgttcgcgc tgacgatttt ttgggcctat atcgcttttt cgcagtacct gctcatctgg
     8701 tacgccaaca ttcccgaaga gaccatctgg ttccgccatc gcctggaggg aagctggacg
     8761 tggatgagcg ccgcgctgct gatcggacat ttcatggtgc cgtttctggt gctgctaccc
     8821 agctcgtcta aacggaattt gaaaattctg gggctgatgt ccgcatggat tctggcggcg
     8881 cactttatcg atctgtactg gcagattatg cccaacttcc atgcgcacag agtcgagttc
     8941 cactgggccg atgcagccac ttttgcggcg tcgggtggaa tcgtcagttg ggtcttctgg
     9001 acaggcacgc gggcgcggcc tgctgttccg gtaggagatc cgcggctgga aaagtcgctc
     9061 gaatttgaga acgcgtagga gggctgatgc aagaccacaa acagcatggc ggatacgact
     9121 acggcgaacc acgctccggg ctgattgcgc tcttcggcat tgcagccatt gtcctggttg
     9181 tagcggcagt gttcgccata cagacctact acgatcacgt gcgggagaag catttgtacg
     9241 agaaggtgct tgctcccgta tcggacgatc tgcaatcgtt gcgggcgaaa gaagacggcg
     9301 agttgcactc ctacggctat actgaccgcg atgccggtaa ggtgcgcatt cccatcgaaa
     9361 gggccatgga gcttctggag aaggaagccg cggcaggaaa gctcgcctat ccaaccaggc
     9421 cggcaccggt gaaagtggca gcccaggcag aagcacaacc tcaggaggga gaggcacctc
     9481 atgcgcctgc cagcctggat tagcctgttg ttcattgcgg cacttctgcc ggccgccgac
     9541 ccgcagttgc ccgcggagtt ggagggtgtg ggtgtcgtcg aacggatcgg cgattcgatt
     9601 gacctgggac tgacttttac ggacgaaacc ggccgtgcgg tcgcgctgcg ggagtatttc
     9661 tccagtgacc gcccggt
//
LOCUS       DASTMK010000380         8090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_499456, whole genome shotgun sequence.
ACCESSION   DASTMK010000380 DASTMK010000000
VERSION     DASTMK010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499456"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFQ79_16550"
     CDS             <1..489
                     /locus_tag="VFQ79_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141335.1"
                     /translation="QTTYGMMSGFHGSMLFTLSMPVTRRRLFFVRAGLGAIETCAFVV
                     MTAGLTLLLRPETTGTSQAFLYGLRAMVCTMAVYALSALLACVLDETWQFMGGCLILG
                     FVLAIQSRVAVISDFSPLRGMSLVSYPVTASVPWALVGASVVLIAIFLYTSVLVLQRK
                     EY"
     gene            659..1225
                     /locus_tag="VFQ79_16555"
     CDS             659..1225
                     /locus_tag="VFQ79_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0141336.1"
                     /translation="MITTSEPTIAVPTQVARPVPTDREAFFGLDEIIVSKTNTKGIIT
                     YANRVFMKISGYAEEELLGKPHNCIRHPDMPRCVFKLLWDTISAGSEIFAYVKNLCKN
                     GDYYWVHAHVTPTYDSLGKIIGYHSNRRVPGRKAVSYMEDLYRELRSIESRHSNWREG
                     MAAAGEALNQKLKQAGVEYDEFIFSLED"
     gene            1194..2408
                     /locus_tag="VFQ79_16560"
     CDS             1194..2408
                     /locus_tag="VFQ79_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0141337.1"
                     /translation="MTNSSSPSRTEIGICHASESDPAGYYREWIRRAADVCECAANGD
                     LEQRLLLAPADGDLGRLLHAINHLLDMTDAFVRESGACMDSAARGRFFRNIILRGMRG
                     SFQHAAKLANSATAEMAQKSVALTNLEQRRAGVAKELARVIGSIADSAAEVRTTADAL
                     SSGANTTSEVSTEVSSSAEETSLNVQAVASATEQLTASFAEVERQTKQSEQFARTAVE
                     DAERTAETMDGLSAASRHIDGIIKLITQIAARTKLLALNAAIEAARSGEAGKGFAVVA
                     AEVKNLAQRTAEATEGITEEVHRIQAAISNVAGGIKNIGERINQMDNIGADITQMITE
                     QRQATREISSSVHRAAENTRVVSQQIAGVRETAGSTREYASSLLRSAGELSNQSRQLE
                     ETMKSLLEVGSK"
     gene            complement(2423..2764)
                     /locus_tag="VFQ79_16565"
     CDS             complement(2423..2764)
                     /locus_tag="VFQ79_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141338.1"
                     /translation="MGRKRIEKLDLLQGTLDMMVLRTLSAGPANGYEIAKAIERSSED
                     VLNVDHGSLYPALHRLEKSRLVTGKWQISSTNRRARYYRITPAGRKRLVVERSKWEQL
                     ASAVSRVMRPA"
     gene            2971..3576
                     /locus_tag="VFQ79_16570"
     CDS             2971..3576
                     /locus_tag="VFQ79_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141339.1"
                     /translation="MDFSRRLFLGAAAAAAARSPATAKKEEVYRFRTEEADIEMAVQF
                     HDEYTSRGFWFGERNSDRRYCLSAEGEEGRNCVTAFRGSIAIARYRVRPRSLRHDARM
                     LREYVRTVDYDARLAHRPPFERTIVLENGLGSDLQAFGYEAGPDDRSNPETHGPWYLC
                     RQDLYLDPQPTPFLILYWKHALPSIRLLDLIPGEQTWVVKK"
     gene            3558..6275
                     /locus_tag="VFQ79_16575"
     CDS             3558..6275
                     /locus_tag="VFQ79_16575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141340.1"
                     /translation="MGREKVATWFSGLVSRVRHYRTDDDLKDELQAHLEMQEEDHLGA
                     GMSVHEARRRARLALGSAGTVVENVRDQEFITMLESCYRDFVLGIRSLRKSPVFCLAA
                     VLTLAIGIGANTAVFTLLHGLLLRSLPVADPGQLVRLRLARADASQWAATHTVPYRML
                     PSLQAQQRSLSGIAAWRIFLATVEDRDGTPGYYHVGLVGGNGFKVLGMTAYAGRLINE
                     SDDVRGGPAEGWSTVLSYGFWKDRFGGDPDIIGKSINVANTALTVVGVAPRDFQGLWP
                     GFDPKLYLPMQFVNVLVGRDVLNTPASFATCAVVGRLNPGVSLEEAAAEIAVHQGQLL
                     REFLPPEYERDPGFQGAKLQVEPARTGLPTFFQTAYSTPLYLMQGLVAFVLLLCCVNV
                     SGLMMSKIHERRREFAIRTAIGAARWRLVRQYLTESFVIALAGAALGSVAAWYGSGLL
                     LLFFRDPNFGFGMSVEPNNTVFAVTAASAVLTTLFFGLIPAWKMGRSDPGMLLKTRTA
                     AAHRHAGGRALVPIQVSLSLILVALATLLSQSLLRLQGEYTGFDVDHVTIQTPPFHLL
                     PQTGDSRLDVYQRMVDRIERSPAVRSAAITRNTPMTGVQSTAVFQALVEGASHTGNVT
                     MAYNDVGPGYFRTMGTKIVAGREFFKHERRRDVCILNQAAANYLFPRRQALGQYVRTA
                     DPQEFPQPATCLIVGIAEDAKYWSLREPPPPTLYFPITTYALEKAFNFVFLMNSPHKA
                     QAVAAYREALQEIAPTVPLVLFATLREQMEAALGSQRAITTLSNFFGGVALFLSAIGL
                     YGLLSSGVAQRTGEIGIRVALGAQRRTILWMILSDAFRLVGAGMLVGAFGLYFAVRYV
                     KNMLYGVSAFDPVTLAAMVAMLAAVALIAALVPALRAASVDPIRALRIE"
     gene            complement(6280..6654)
                     /locus_tag="VFQ79_16580"
     CDS             complement(6280..6654)
                     /locus_tag="VFQ79_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU0141341.1"
                     /translation="MNEARIAKFRKALIARERELQQRLEHPEGISVQQQAEQQDVGRQ
                     RLDIDVSIESLAVEWELLRNIREALHRMERGTYGICGVCDGPVGIRRLNALPWAPFCV
                     KCQSAMESGDILCLPGSQPAAA"
     gene            6795..7235
                     /locus_tag="VFQ79_16585"
     CDS             6795..7235
                     /locus_tag="VFQ79_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141342.1"
                     /translation="MRTQWARWFMAAFALLLLSCEDKEAYLALQREVNEIGMQVDLIE
                     QSIEQRLPDTVEILEQALKNVTAANARLDILRTEFTDRLDRRRQTFSEPIAELTGTLD
                     RLTEDLSALRKDASDLGAGIDRLQHQVSTLKSEITVIQPSPPGF"
     gene            7239..>8090
                     /locus_tag="VFQ79_16590"
     CDS             7239..>8090
                     /locus_tag="VFQ79_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU0141343.1"
                     /translation="MSFPSRSVEGSQRLGAEYLGAATTRFLVWAPYPQLVQLRLFLPE
                     ERLIPMEAVGDGYYQAILKGVTTGTHYKFHLDSGDFPDPASRLQPLGVHGPSEVVEND
                     FAWTDQNWAAPPLKDLILYELHVGTYTAEGTFDAIIETLDQLKETGISAIQLMPVAEF
                     PGARNWGYDGVYPYAPHSAYGGVKGLKRLVNAAHECGLAVILDVVYNHLGPEGNYLWQ
                     YGPYFTEKYQTPWGPAVNFDGEDSRPVRRYFIENAVHWIRDYHFDGLRLDAVHAIFDS
                     STPHILEE"
BASE COUNT         1599 a         2358 c         2395 g         1738 t
ORIGIN      
        1 caaaccactt atgggatgat gtcggggttt cacggctcta tgttgtttac tttatcgatg
       61 ccggttacgc ggagacgatt gttctttgtg cgggccgggc tgggagcaat agagacgtgt
      121 gcctttgttg ttatgacagc cggtttgaca ctactcttgc ggccggaaac aacaggtaca
      181 tcccaggctt tcctttacgg tctgcgggcg atggtctgta ccatggcagt gtatgccctt
      241 tccgccctgc tggcctgcgt gctggacgaa acgtggcagt ttatgggcgg ctgcctgatt
      301 ttgggctttg ttttagcgat tcagtcccgg gtggctgtga tctcggactt cagcccgctc
      361 cgaggcatga gtttagtgtc ctatccggtc accgcatccg tgccctgggc tctggtcggg
      421 gcctccgtag tgcttattgc catttttctg tacacctccg tgctagtgct tcagcggaag
      481 gaatactgag cggaagtacc gttttcggat tcaaaatcgg catagctatt ccgatatgga
      541 acgatatgtg gaccttgacg gacggaaaat gccgcaaagt cgatacccag cgtttggcgt
      601 gttgcgtgcc tttgccgaga tcgcggcctg tggtcgccaa atttgttgag agggctttgt
      661 gatcacgact agcgaaccaa ccattgctgt gccgacgcag gttgcccggc cggtgccgac
      721 cgaccgggaa gcctttttcg gcttggacga gatcatcgtc agcaaaacca acaccaaggg
      781 gatcatcacg tacgccaacc gtgtctttat gaaaatctcc ggttacgcgg aagaagaact
      841 gctcggtaag ccgcacaact gcatacgcca tcccgacatg ccgcggtgcg tattcaagct
      901 gttgtgggac accatcagcg cgggaagcga gatcttcgcc tacgtcaaaa acctgtgcaa
      961 gaacggcgac tattactggg tccacgcgca tgtcaccccg acatacgatt ccttggggaa
     1021 gatcatcggc tatcactcga accgtcgtgt tcctggccgt aaagccgtgt cgtacatgga
     1081 agacctctat cgggaactcc gctccatcga aagccgccac tctaactggc gtgagggcat
     1141 ggcggcagcc ggcgaagcac tcaatcagaa attaaaacag gctggtgttg aatatgacga
     1201 attcatcttc tccctcgagg actgaaatcg ggatctgcca cgcgagcgag tcggatccag
     1261 ccgggtacta ccgcgaatgg attcgccgcg ctgccgacgt ctgtgaatgt gccgccaacg
     1321 gcgatctgga gcagcgattg ctgctcgcac ccgcggacgg tgacctcggc cgccttcttc
     1381 atgccatcaa ccatctgctg gatatgacgg acgcgtttgt gcgtgaatcc ggcgcatgca
     1441 tggattcggc cgctcgcggc cggttcttcc gcaacatcat cctgcgtgga atgcgaggca
     1501 gcttccagca cgcggcaaag ctcgccaact cagcgacagc cgagatggcg caaaaaagtg
     1561 tcgcattgac gaatctggag caacgacgcg cgggggttgc caaagaactc gcccgtgtta
     1621 tcggaagcat cgccgattcg gcggcggagg tccggacaac cgcggatgcg ctttcgagcg
     1681 gcgccaacac caccagtgag gtctcaacgg aggtgtcttc gtctgccgaa gagacatctc
     1741 tcaatgtgca ggcggtcgcc tcggccaccg agcaactgac cgcctccttc gccgaggttg
     1801 aacgtcaaac gaaacaatcc gaacagttcg cccgcactgc cgtcgaagat gccgaacgta
     1861 ccgctgaaac gatggatggc ctttcggctg cctcgcgcca tatcgacggc atcatcaaat
     1921 tgattacaca aatcgccgca cggacgaaac tcctggcgct taacgcggcg atcgaagcag
     1981 cgcgctccgg cgaagcggga aagggtttcg cggtagtagc cgcggaagtc aagaacctgg
     2041 cacaacgcac cgccgaggca accgaaggca tcaccgagga ggtacaccgc atccaggcgg
     2101 cgatttctaa cgtggccgga ggcatcaaaa acatcggtga acgcatcaat cagatggaca
     2161 acatcggagc ggacatcact caaatgatca ccgaacagcg tcaggccaca agggagatca
     2221 gcagtagcgt tcatcgcgct gccgaaaata cccgggtggt cagccagcag atagcagggg
     2281 tccgggaaac ggcggggagc actcgggagt acgcctcttc actactccgg tcggctggcg
     2341 agttgtcgaa tcagtcccgg caactggagg agactatgaa aagccttctc gaggttggtt
     2401 cgaaatagta tcgcggtcgg cctcaggccg gccgcatgac gcggctgacg gcggaggcta
     2461 actgttccca tttggatcgt tccacgacca gccgtttgcg gcctgccgga gtaatccggt
     2521 aataccgtgc gcggcggttt gtggaagaga tctgccattt ccccgtgacc agacggctct
     2581 tctcgagccg gtgcagcgcc gggtaaagag acccgtggtc gacgttgagg acatcctccg
     2641 acgagcgttc gatcgctttc gcgatttcat aaccgttggc ggggcctgcc gaaagagtac
     2701 gcagcaccat catgtccagc gtaccctgta gcagatccag cttctctatt ctcttcctcc
     2761 ccatttccgc cttgctatgg tagcatgagg caatagtagt atggcaagag gtagattaac
     2821 tactattcga aaacggacag tggctgtatc gaaaccggac agccggactg gagagacgaa
     2881 aacggcgggt gggcgtttat tgaacaaaac gaacggttcg ctgcaatggt gggcgggatg
     2941 cattgccgga cggccaggag gtcattgacg atggacttca gccggcgatt gtttcttggc
     3001 gccgccgcgg cagctgccgc caggtccccc gccactgcga agaaggagga ggtataccgc
     3061 ttccggactg aggaggccga catcgaaatg gcggttcagt tccacgacga atataccagc
     3121 cgcggcttct ggttcggcga gcggaattcg gatcgccgct attgcctctc ggcggagggg
     3181 gaagaggggc gcaactgtgt aaccgcattt cgcggctcga tcgcgattgc gcgctaccgg
     3241 gtgcgcccgc gatccctgcg ccacgacgca cggatgctgc gcgagtatgt tcgcaccgtc
     3301 gactatgatg cccggcttgc ccaccgcccg ccgtttgaac gcactattgt gctcgaaaac
     3361 ggactcggca gcgatttgca ggcattcgga tatgaggccg gtccggacga ccggtcgaat
     3421 cccgaaacac acggtccctg gtatttgtgc cggcaggacc tgtacctgga tccgcaaccc
     3481 actccctttc tcatcctgta ctggaagcac gcgttgcctt cgatacgatt gctcgacctg
     3541 atccccggag agcagacatg ggtcgtgaaa aagtagctac gtggttctcc ggtctcgtct
     3601 cacgagtccg gcactatcgc accgacgacg atctgaagga cgaactgcaa gcccatctgg
     3661 aaatgcagga ggaggaccac ctgggcgccg gtatgtctgt ccacgaagca cgccggcgcg
     3721 cgcgtcttgc tttaggaagt gccgggaccg ttgtcgaaaa tgtccgggac caggagttca
     3781 tcacgatgtt ggaaagctgt taccgcgact ttgtgctcgg tattcgttct ctgcgaaaga
     3841 gcccggtctt ttgtcttgcc gccgttctca ccctggcgat cggcattgga gccaataccg
     3901 cggtctttac cctgcttcac ggcttgctgt tgcgtagctt gccggtcgcc gatccaggac
     3961 aactggtccg cctccgtcta gccagggccg atgcctccca atgggctgct acgcacaccg
     4021 ttccctatcg tatgcttccg agtctccagg cccagcaacg ctcgctttcc ggaatcgcgg
     4081 cgtggaggat ctttttggcc acggtggagg accgggatgg gacgcctggc tactaccacg
     4141 tagggctggt gggcggcaat ggatttaaag tgctcggaat gactgcgtat gccggccgtt
     4201 tgatcaacga atccgacgac gttcgtggcg ggccggccga gggttggtcc accgtgctca
     4261 gttacggttt ctggaaagac cggttcggtg gcgaccccga catcattggc aagagcatca
     4321 acgttgccaa caccgctttg acggtcgtcg gtgttgcccc gcgtgatttc caggggctat
     4381 ggccggggtt tgatccgaag ttgtacctgc cgatgcagtt cgttaacgtg ctagtgggcc
     4441 gggatgttct caatacgccg gcatccttcg cgacttgcgc tgtcgtggga cgcctgaacc
     4501 ccggcgtttc tctcgaagag gccgccgcgg agatagcagt gcatcagggg caactgctgc
     4561 gcgagttcct gccgcccgag tacgaacggg atccgggatt tcagggagcg aaactgcaag
     4621 tggaaccggc ccgcacagga ctgcccacat tttttcaaac ggcctactcc acccctctgt
     4681 acctgatgca agggctggtc gcgttcgtgc tgctgctttg ctgcgtcaat gtcagcggcc
     4741 tcatgatgtc caagattcac gagcgccggc gtgagttcgc cattcggacc gctattggcg
     4801 ctgcgcgatg gcgcctggtc cgccagtatc tgacggaaag ttttgtgatc gcgctggccg
     4861 gcgccgcgct tggatcggtg gcggcatggt atgggagcgg gcttttgctt ctgttcttcc
     4921 gcgatccgaa ttttggcttc ggaatgtcgg tggaacccaa caacacagta tttgccgtga
     4981 ccgcggcatc ggcggttctg acgacgctgt tctttggctt gattccggcg tggaaaatgg
     5041 ggcgatcgga tccaggcatg ctcctcaaga cgcgtacggc ggcggcgcac cggcatgccg
     5101 gcggacgcgc tctcgttccg attcaggtgt cgctatcttt gatcctggtt gccttggcta
     5161 cactgctctc tcaaagcctc cttcgccttc agggcgagta caccggcttt gacgtggacc
     5221 acgtgacgat ccagacgccg ccctttcatc ttctgccaca gacaggcgat tcgcggcttg
     5281 acgtctacca gagaatggtg gaccggatcg aacgctcgcc tgccgtccgg tcggctgcaa
     5341 ttacccggaa cactccgatg acaggggtac aatccactgc ggtgtttcaa gcgctggtag
     5401 agggcgcgag tcacaccggg aatgtgacca tggcctacaa cgacgtaggg ccaggttact
     5461 tccggaccat gggaacaaaa attgtggccg gccgcgagtt cttcaaacac gagcgccgcc
     5521 gggatgtgtg cattctgaat caggcggctg cgaattacct ctttccccgc cggcaggctt
     5581 tggggcagta tgtgcgaacc gccgatccgc aggaatttcc gcagcctgca acctgcctca
     5641 tcgtcggaat cgcggaggat gcgaaatact ggagccttcg cgaacctcct cctcccactc
     5701 tctatttccc cataaccacg tacgccctcg aaaaggcctt taacttcgtg ttcctaatga
     5761 attcgcctca taaagcccag gccgttgccg cttaccgcga ggcgttgcag gagatcgctc
     5821 caaccgtccc actcgtcctg ttcgccaccc tccgcgagca gatggaagcg gcgctcggaa
     5881 gccagcgggc gattacgacg ctaagcaact tcttcggtgg ggtagcctta tttttgagcg
     5941 ctattgggct gtacggcctc ctgtcctccg gcgtcgctca gcgcacggga gaaatcggca
     6001 ttcgtgttgc cttgggcgcc caacgccgga ccatactgtg gatgatcctt tccgacgctt
     6061 ttcgcctggt aggagcaggg atgctggtgg gtgcattcgg cctgtatttc gccgtgcggt
     6121 atgtgaagaa tatgctctac ggtgtttcgg cgttcgatcc ggtgaccctg gctgcgatgg
     6181 tggcgatgct ggcggcggtc gcgctgatcg ccgcgcttgt gcccgcacta cgcgcggctt
     6241 ccgtcgatcc cattcgtgcg ctgcgtatcg agtgatgact caggcagccg cgggctggct
     6301 gcccgggagg cacaagatat cgcctgactc catagcggac tggcatttca cgcagaaggg
     6361 agcccagggc agcgcattca ggcggcgaat gcctacaggc ccatcgcaca cgccgcaaat
     6421 accgtacgtg ccgcgctcca tccggtgaag cgcctcgcga atgttgcgca acagttccca
     6481 ctccaccgcc agactttcta tggacacgtc tatatcgagg cgctgccgtc caacgtcctg
     6541 ctgttccgcc tgctgctgaa cggaaattcc ctccgggtgt tccagccttt gttgcagttc
     6601 ccgctccctg gcaatcaacg cctttcggaa tttcgcgatc cgtgcctcgt tcatgacatc
     6661 tcctcctttt atgctaaaaa ggtggtctcc cggacggtat cccccacata cgggagatgg
     6721 cttccttttg agcactaggg ccggttcagc gaatggaatc agtgcgtttt cgtacttccc
     6781 ggtataatga aaatatgagg acgcaatggg ccaggtggtt catggcagcc ttcgccctcc
     6841 tccttctatc ctgcgaggac aaggaggcgt accttgcact tcaacgcgaa gtgaatgaga
     6901 tcggcatgca agtcgatttg atcgagcagt cgattgaaca gaggctgccg gatacggtcg
     6961 aaatcctgga gcaggcactg aagaacgtga ctgcggcgaa tgcgcgtctg gacatcctcc
     7021 ggacggagtt taccgatcgc ctggaccgcc ggcgtcaaac gttttcggag ccgatcgctg
     7081 agttgaccgg gacgctggac cggctcacgg aagatctttc ggctctacgg aaggatgctt
     7141 cggatcttgg tgccggaatc gaccgtcttc agcaccaggt ttcgactttg aaatcggaga
     7201 tcaccgtcat tcagccgtcg ccgcccggct tttaggttgt gagttttccc tcccgatctg
     7261 ttgaaggatc ccagcgatta ggagcggagt atctgggcgc cgctaccacg cgttttctgg
     7321 tttgggcgcc ttacccccaa ctcgtacagt tacgcctgtt cctgcccgaa gagcggctaa
     7381 ttcccatgga agccgtggga gacggctatt atcaggcgat cctcaaaggc gtcacgacgg
     7441 gcacgcacta caagtttcat ctcgacagtg gggacttccc ggaccccgcg tcgcgtttgc
     7501 agccgctggg agtgcacgga ccctcggaag ttgtggaaaa cgactttgcc tggaccgatc
     7561 agaactgggc tgcaccgccc ttgaaggatc tgatccttta tgaactacac gtaggaacct
     7621 ataccgccga aggaacgttc gacgccatca tcgaaaccct cgatcagtta aaagaaaccg
     7681 gtatttcagc cattcaacta atgccggtgg ccgagttccc gggagcgcgg aactggggct
     7741 acgacggggt atatccctat gcgccgcact ccgcctatgg cggcgtcaaa ggattgaaac
     7801 ggctggtgaa tgcggcgcac gaatgcggac tcgcggttat tctggacgtc gtttacaatc
     7861 acctcggtcc tgagggcaat tacctatggc agtatggccc gtatttcacg gaaaagtacc
     7921 agacgccctg gggcccggcg gtgaatttcg atggcgagga cagccggccg gtgcggcgct
     7981 acttcatcga aaacgccgtt cattggatac gcgactacca cttcgacgga ttgcgcctgg
     8041 acgcggtcca tgccattttc gattcgtcca cgcctcacat cctggaggaa
//
LOCUS       DASTMK010000381        14564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_114844, whole genome shotgun sequence.
ACCESSION   DASTMK010000381 DASTMK010000000
VERSION     DASTMK010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114844"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VFQ79_16595"
     CDS             <1..1165
                     /locus_tag="VFQ79_16595"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEU0141344.1"
                     /translation="QPDDAHLSGVVLRLNPDGTAPQDNPFYRHGAQLGGQAGANLQKV
                     FAYGIRNSFGMEFDPFSGELWETEHSDDTFDEINRIEAGHNGGWVQIMGPISRIDQFK
                     AIETSNEFFGLQQLRRPPTNPADSPAAALSRLVMLPGAHYADPQFSWKYATLPVALNF
                     APQTFGKDLARKMIVGIASGSGYLFAFGLTGNRRGLAFEDSRLSDHVADNTAKQDLTE
                     SESLVLGSGFGILTDIETSPANTLYLVSFTQGSIYELSRRSGNSFDDNSGTGKPLVTR
                     LTGAVEIPPGDPDGTGLATFRLNSGQGEICYELTVRNIATATAAHIHPGAAGQTGPAI
                     VPLNAPASGSSSGCVAVPRDLVKAIRQDPDAYYVNVHNAEYPAGAVRGQLSGQ"
     gene            1206..1460
                     /locus_tag="VFQ79_16600"
     CDS             1206..1460
                     /locus_tag="VFQ79_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141345.1"
                     /translation="MPLLEEGVFSNPRMSLKPEVIPMQMILKIWGPTGVRREGAAVER
                     PSLCISTVLLPEPVSQGSASRQQLTSCLAHEGLRREARRT"
     gene            1981..4611
                     /locus_tag="VFQ79_16605"
     CDS             1981..4611
                     /locus_tag="VFQ79_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141346.1"
                     /translation="MASASAISPLDVAPPKRWRRFVPPWLRSLIWIDTADRYDAFLSY
                     SWKSDSKVAPVVQSLIQRFLRPWYRLRAKTVFRDLSCLPAGSSLEAELFDRLDRSKHL
                     IVLASTEAATSRGMETEAQYWFSRKRDGEVIIIISSGDAEGWEEIRECLIPPSLRMNL
                     PSAPVWASIRQRRDRILANPADQRLRAELIEDLKQVFLRFYPDRDWGQLRGEERSQRR
                     RVLGLLSCVSLLLMTLTAFSWFLRLEAVQQRSLAEHRQRIAVSRQLAAQATATIAGHF
                     DLALLLSVESYRHEITVESYQSLLAGLEHEPRLAQLVRLPGTRISKPALDATGVVTVV
                     TWDSNRGAAVWDVTAGRPVCQLPSTLDVRSGQVKLTPDARAFAHADSAGAVSIWDARM
                     CRTVRPGWVADPEGVRTIAFSHDGSRLVTGGESGTLKIWNLNRQPPEGRVLANAAGLP
                     ITAATFSPDDRVVAFARGPATGVAYADLIEVRLVHLESGRSTKLKGPTGIVRVLAWDD
                     TGGLLASGGCGENPTGINECVSGEIVVWDTRQEVNGHSLPDVAGDVHDLRFVPGATAL
                     FSAGCGEMERDGAGVCAGDISLWDLESVSHAATMSEVRRFIDSIAMNPNGQNFVTIDD
                     EGVAQWWRITNQHPLIEPIESRVVEKGSGSSTRAGRRQSTFLSRSGKVRVTYMDGDIE
                     VENSTTGRSARIRSTNSDLRVEGTAVSEDDRILVSLAQHVNGGETELRLWDVETGRPL
                     SGPLTGHTREIVAAAFNKDGTVLVAGGCGQYATFSGCAGELVLWHVPSGLLLTRPIAT
                     GANVLDVRFDGARAIVEMERPGSEPMMATIDVDIASWAKRACARANRNLSADEWRRYL
                     PDEPLRDTCALQAVTTAERP"
     gene            complement(4719..7487)
                     /locus_tag="VFQ79_16610"
     CDS             complement(4719..7487)
                     /locus_tag="VFQ79_16610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141347.1"
                     /translation="MIRDLLYRLRAVVRRNGMERDLEDELQFHIERETEKLERAGLSH
                     EEARRRVNLDFGNAEQVREECRDARGIAFVESILRDLQYASRVLRANPGFTAVAVLSL
                     TLGIGATTSIFTVVNAVLVRTLPVSDPQQLMVAYWKTDVPVRMPTQNGVNRKDPASGQ
                     WLNSTFTLAALRQFRESGAGMLDVFGFYVPGHVRVSDGTTMWPAHCTFVTGNFFEAIR
                     ARIALGRPLTEEDDRNGATAAVVTYDFWQHSLEGDASILGKVLRVAGVPVTVVGVSAP
                     GFYGVASAGWGGPTELFLPLNAMDTVMPRELRTGRPKTAPDFSWVQIFARKHPGVTTK
                     AAAARLTALFRGTFAASGVPALEQARNPRVVLLDGDKGLAQLRDSIQRPLLILFGMVV
                     LVLLLACVNVANLQLARSAARHREVAVRLSLGAGRARIVRQLLTEGFLLSAAGAALGL
                     LLAVAGSRLIAAQLTGNATVALDLAPDLTVLAFTTLVSVTSAVLFGLVPALKASRVEI
                     APNLKQGGRTAGARRSVGWHGAVMGRGLIAIQVALSVVLLAGAGMLLRTLDNLAHVDA
                     GFVRDRVLTFRLDAGELGYQVAQAGPLYDRVLELIRAIPGVVSAASLSQPIIGGGVDS
                     TDVSLPDIENGRRINLWMNTVSPEFFQTMGVPVVQGRALSRQDTATAPNVVVLNQTAA
                     RRLFGERPAIGQVLWRHDHHDDKARWQVQVVGVVRDAKYNSLRTAPPPTVFVPYAQDH
                     RPWTGMAFAVRTAGDPAAMAGPVRQAIARVNRDLNMTDVKTQRRLIEDSLYEERLYAL
                     LLTLFGLFALVLAAIGLNGVTAYATSRRTSEFGLRMALGAQRGQILRLILRQVLAAVI
                     LGMAVGVGGMWAASRWIASMLFGVQPMDPVSIVFVLLLLAAVASGAAFMPAWRAARLD
                     PMVALRAE"
     gene            complement(7499..7831)
                     /locus_tag="VFQ79_16615"
     CDS             complement(7499..7831)
                     /locus_tag="VFQ79_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141348.1"
                     /translation="MPAERIDLPQGTLDLLILKSIALEPQHGWDISERIRQMSKDILQ
                     VQQGSLYPALHRLERRGWIKGKWEVTRNNRRAKYYQLTTAGRKQLEQETRYWRMLVAG
                     VGGVLETT"
     gene            8001..8987
                     /locus_tag="VFQ79_16620"
     CDS             8001..8987
                     /locus_tag="VFQ79_16620"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0141349.1"
                     /translation="MQAAVVSAVNTSWQVKEVPQPQPGPNQVLVKMHASGICYTDVHQ
                     TLGRLPGPFPRILGHEPVGEIIAVAPGVTTRKVGDRVGTAWIQSTCGRCEWCQRGRRM
                     FCPYLKGTGVEVQGGHAEYMLMNTDATYLIPGKVSYEQAAPIFCAGYTVYSGLRWADP
                     KPHERVAVLGIGGLGHLAVQYAKAAGFETIAVSHSPDKDKMIRDLGADEVVRDGKSLA
                     AVGGADVILSTSNSTKSMVDSLQGLRPDGRLVTMGADVEPLSVSLADLIGKRIRIIGS
                     QQNGPEYLYEALDFVAQGKVKTIVETYPLTQAVKAYDRVAEGKARFRVVLTM"
     gene            complement(9034..12288)
                     /locus_tag="VFQ79_16625"
     CDS             complement(9034..12288)
                     /locus_tag="VFQ79_16625"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141350.1"
                     /translation="MLQRKTRMLFALALMLGLPAVLLGQAAVSGSLTGKITDEAGALI
                     PNAEVVLTNEATGVKFVTRTNEAGSYTFTNLSPGRYTLSAQMTGFSPVEVSGLTVTVA
                     QFARADIQMKVGVVSERVEVTARAGVLETERPTLSSVITHTQVTQLPFNGRYNLGGLI
                     ALAPGVQRGGRFVSGGFFTGGLNVVVDGAVNLDMQNGGPSAADPTFDSIEEFRFITNT
                     ASAEYSHGTAQLIVVTRSGANDLHGSLFWFNRNREFAAKYTLDHREKPPYNRNEFGGT
                     LGGRIIKDKLFYFGSFEQLSQREWATRTFNMATVSMKQGNFAGLRTITDPLTSAPFPE
                     NRMPGSRISPAATFFMGYMRDPNLEGTGPAGTGVNFIGRNQTKQDSPRIGIKMDYHPT
                     SNDILSGGAKIFNDGPYLRAGTGPELFGNYTQGFDQRQYFAAYTKIISPRLTNEVRLG
                     VTTPYGYHLTNEHNDFDPGTLFPQLDGQCCAEKRSPDKNYDGRFGGGLPGVNITGYQG
                     ISTVGAHAKDNRIDINIFNNLVYIRGTHQLKFGGYYYRYQGYWGGIYPHGLGIFGFTG
                     RYTGDAFADFLLGYANESQRGPAYQFGDFNEKRFGGYVQDDWQVTRNLTLNLGIRWDV
                     ETPFKEKKLRQANYDPASNRLVTYSGTDQFPPEVVTRLAESYPVVTSVQAGLGTTTLT
                     RDTNWRNFQPRVGFAWRPFGDSSTVIRSGAGIYYAYEASNQLGLRQVLFAPFALIENY
                     NSPPGNNPYVTFADPFPGIGSIPSSPSILGMPRDFPNPQSLQWNFTIERELVPNTSLR
                     ISYVGNQTHFAAQEYDLNLPRAFGPGTLQTLRPLQPWGDIRWIDPRGNAVTHQLQIGS
                     TRKAGDLTYQFEYQFTSSISDGAGAADGWGSVGGIDYPFDARRNRGPMDGIVRHQAVT
                     NWVYNLPFGRGRKLFSGVPAPLNHVIGGWQVSGIATLRTGTPYHVSYSGQLVGFPTSG
                     RADVVGDWRVSDPGVEGWFNPNAFRQPAPFTLGNVGRNSMWTPGAWFLDAGIMKNFRF
                     AERYDIQFRSEFFNMLNHPNPGGPGANLSVPSTFGKTTSFTAARVILFGLKFAF"
     gene            12565..13806
                     /locus_tag="VFQ79_16630"
                     /pseudo
     CDS             12565..13806
                     /locus_tag="VFQ79_16630"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            complement(13820..14488)
                     /locus_tag="VFQ79_16635"
     CDS             complement(13820..14488)
                     /locus_tag="VFQ79_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEU0141351.1"
                     /translation="MRLPVLLVCINAVLFSASKDDLNRLNESAAVLEEVMAAPDKAIP
                     QDLLDRSHCIVVVPGLKKAAFIIGGKYGRGFLSCRKAGKVGWSAPGAVRVEGGSFGFQ
                     LGGSETDVVMLVMNERGADRLLSSKFTVGGDASAAAGPVGRTASAQTDATMRAEILTW
                     SRSRGLFAGVSLQGATLRQDISVNEGIYGKAYANRDVVNSGLAVPAGASRLISLLNKY
                     SSRK"
BASE COUNT         2955 a         4288 c         4439 g         2882 t
ORIGIN      
        1 gcagcctgac gatgcacatc tatccggcgt cgtgctgagg ctgaatcccg atggcacagc
       61 gccccaggac aatccttttt acaggcacgg cgcgcaactc ggaggacagg ccggcgcgaa
      121 tctgcagaag gtgtttgcat atggaatccg gaacagcttc ggcatggaat tcgatccgtt
      181 cagcggtgag ctttgggaga ccgagcacag cgacgacacg tttgacgaga ttaacaggat
      241 cgaggccgga cataacggcg ggtgggttca gattatgggt cccatcagcc gtattgatca
      301 gttcaaggca attgaaacga gcaacgagtt ctttggattg cagcagcttc ggcggccgcc
      361 gaccaatcct gccgacagtc ctgcggcggc gctatcgcgc ctggtgatgc ttcccggcgc
      421 gcactacgcc gacccgcagt tcagttggaa gtacgctacg ctgccggttg cattgaattt
      481 cgcgccacag accttcggca aggatctggc ccggaaaatg attgtcggga ttgcttctgg
      541 ttcagggtac ctgtttgctt tcggtcttac aggcaaccgt agaggtcttg cattcgagga
      601 ttcccggctt tcggaccacg tcgcggacaa taccgcgaaa caggacctga ccgagagcga
      661 gagcctggtg ctcgggtccg gattcggcat tctgacggat atcgagacgt cgccggcgaa
      721 tactctgtat ctcgtctcct ttacacaagg cagcatctat gagctgtcca ggcgttcagg
      781 gaactcgttc gatgacaaca gtggaacagg taaaccgcta gtgacccgat tgactggagc
      841 ggtggagatc ccgcctgggg atcccgatgg caccggcctg gcgacgttcc gcctgaacag
      901 cggccagggg gagatttgtt atgaactgac ggtccgaaac attgccactg cgacagcggc
      961 gcacatccac ccgggtgcgg cgggtcagac cggtcctgcg attgtgccct taaacgcacc
     1021 ggcgagcggt tcgtcgtcgg gatgtgtcgc ggtcccacgg gatttggtca aagcgatcag
     1081 gcaggatccg gacgcgtact atgtgaacgt gcacaatgca gagtacccgg cgggcgcagt
     1141 tcgaggccag ttaagtgggc agtagttagc gcagcactgc aaacgagatg ttctggagcg
     1201 gacgggtgcc cttgctcgag gagggagtat tttccaatcc gagaatgagc ctgaagcctg
     1261 aagtgattcc aatgcaaatg atcctgaaga tctggggccc aactggagtg cggagggagg
     1321 gggcggcggt tgagcgccct tccctctgca tcagcactgt attgctcccc gagcccgtct
     1381 cacagggctc ggcttcgcgc cagcaactga cctcgtgcct ggcgcatgaa gggcttcgcc
     1441 gtgaagcgcg ccggacgtga agggcaatct gattatgcta agttgaggag gaaatggaat
     1501 ctgccggcgt ctggttggcc ttcgctacga tcatctgtat gctcgcgctt ctccttcgac
     1561 gttcggtcct ggattcgatg atcctgggaa ttcgggcgtt cgcggacgca atcagcaacg
     1621 gcggaaatcc accaacaccg atgcaccctt ctccttcgaa cgattccgct ctacttcgaa
     1681 gaccacggga caacagccgg gcaccctctg catagcgcgt gatctgatcc gaggctggtg
     1741 gttgcgtgtg acgcttgtat ctaaacgaag aagagaagat gagcatccgg accgtcatat
     1801 caatcttggc aggacggtgt tccacctgat tggcctccgc tggggcaacc agttacggat
     1861 cacgtacggg gtcgcgcgcg atcatgcacg gcgattaggc acggggattg ccttggcaaa
     1921 gcacgcatga tatagaatcc gacagtgaca tcgttgcctt gacgacctca gggcgaaggt
     1981 atggcttccg cctctgccat ctcgccgctc gatgtggctc cgccgaaacg ctggcggcgt
     2041 tttgtgcctc cgtggttgcg gtctctcatc tggatcgaca ccgcggacag atacgacgcc
     2101 tttctctcct atagctggaa gtcggacagt aaagtcgctc ccgtcgttca gtcgctgatc
     2161 cagagatttc tccggccgtg gtacaggttg agggccaaga cagtgtttcg cgatctgtcc
     2221 tgtttaccag ccggttcgag cctcgaggct gaactattcg accgtttgga ccggtcgaaa
     2281 catctcatcg tgctcgcttc cacggaagct gctacgagcc gcggaatgga aaccgaggcc
     2341 cagtactggt tcagccggaa gcgggatggc gaggtgatca tcatcatctc ttcgggcgat
     2401 gccgaaggtt gggaggagat tcgggagtgc ctgattccgc catcgctgag aatgaatctc
     2461 ccgtcagcac cggtgtgggc ctcgatccgg cagcggcgtg atcggattct ggccaatccg
     2521 gcagaccaac ggcttcgagc agaactcatc gaagatctca agcaagtttt tcttcgtttc
     2581 tatccggatc gcgactgggg acaactccgc ggcgaggagc ggtcgcagcg ccggcgtgtt
     2641 ctcgggttgt tgtcgtgtgt ttcactgctt ctcatgactt taacggcgtt ctcgtggttc
     2701 ctgcggctcg aggccgtgca acaacggagc ctagcggaac accggcagcg cattgcggtt
     2761 tcgcgccagc ttgcggcgca ggcaaccgcc accatagctg gtcacttcga tctggctctg
     2821 ctgctaagcg tcgagtctta tcgccacgag attacggtag agagctatca gtcgctcctg
     2881 gccggtctgg agcacgagcc gagactggcg cagttggtca ggctacctgg aacacggata
     2941 tcgaaacccg ctctcgatgc gaccggagtt gtgacggtgg tgacatggga cagtaatcgc
     3001 ggagcagccg tgtgggatgt gactgccggc agaccggtgt gtcaactacc ttcgacgctc
     3061 gatgtccgct caggacaagt gaagctcacc cctgacgcac gagcgtttgc acacgcggat
     3121 tcggcagggg cagtttccat ttgggacgcg aggatgtgcc gaacagtacg gcccggttgg
     3181 gttgccgatc cagaaggtgt tcgcaccatc gcattcagcc acgatggatc tcgactggtt
     3241 accggcggtg aaagcggaac cctgaagatc tggaacctga acaggcagcc gccagaagga
     3301 cgtgtccttg caaacgcggc tgggctgccg atcacggctg ctacgttcag ccccgacgac
     3361 cgtgtggttg ctttcgcacg cgggccagct accggggtcg cctatgcgga cctgatcgaa
     3421 gtacggctgg tgcacttaga gagcggacgc tccacgaagc tgaaaggtcc aaccggaatt
     3481 gtgcgggtcc tcgcgtggga tgacacagga ggcttgctgg cgtcgggggg ctgtggggaa
     3541 aatccgacgg ggattaatga gtgtgtgagt ggcgagatcg tcgtgtggga cacacgccag
     3601 gaggtaaacg gacactcatt gcctgacgtt gcgggggatg tacacgatct ccggttcgtg
     3661 cctggcgcca ctgccctgtt ttcggccgga tgcggagaga tggagcgcga tggcgcaggc
     3721 gtctgtgcag gcgatatctc gctatgggac ttagaatccg tgagccacgc ggcgacgatg
     3781 tccgaggtca ggcggttcat cgattcgatc gcgatgaatc cgaacgggca gaacttcgta
     3841 acgatcgacg atgagggagt cgcacagtgg tggcgtatca ccaaccagca tccgctgata
     3901 gagcccatcg aatcacgggt cgtcgagaaa gggtctggat caagcacccg tgcggggcgc
     3961 cggcagtcga cattcttaag caggagcggc aaggtgcgtg ttacgtacat ggacggcgac
     4021 atcgaagtcg agaactcgac cacaggcagg tccgcccgaa tacgatcgac gaactcggac
     4081 ctgcgcgtcg aagggaccgc cgtgagcgaa gacgaccgga tcctggtgtc actggcgcag
     4141 cacgtgaatg gaggcgagac cgaactgcgg ctatgggacg tagaaacggg gcgtccgttg
     4201 tcgggaccgc tcaccggcca cacgcgcgag atcgtcgcgg ccgcattcaa caaggacggg
     4261 accgtgctgg ttgccggagg gtgcggacaa tacgcgacat tctcggggtg tgctggtgag
     4321 ctggtattgt ggcacgtgcc atctgggctg cttctcacgc ggcccattgc gacgggtgcg
     4381 aacgttttgg atgtacgttt cgatggcgcc cgggcgattg ttgaaatgga gcgccccgga
     4441 tcggagccga tgatggccac cattgatgtg gacatcgcgt cgtgggcgaa gcgagcgtgc
     4501 gcgcgagcaa accggaatct gtctgcggac gagtggcgga gatacctgcc ggacgagcct
     4561 ctccgcgata cgtgtgcatt gcaggctgtt acgacggctg agaggccgtg aactccccgt
     4621 gcgaatctgg tcgtagggtg ccggaacaaa atgcgggtca ctgtcgcgcg tcttgctaac
     4681 agatgacacc ggccagtcgc gtctggccac ctcttgcgtc actcggcgcg tagggcgacc
     4741 atgggatcaa gacgtgctgc gcgccaagcg ggcatgaatg ccgcgccgga agccacggca
     4801 gcaagcaaca acagcacgaa aacgatgcta acgggatcca tcggttggac gccgaagagc
     4861 atcgaggcga tccaccggct cgccgcccac atcccgccaa cgcccaccgc cattccgagg
     4921 attaccgccg ccagcacctg cctcagaatc aggcgcaaaa tctgtccccg ctgcgcaccg
     4981 agcgccatcc gtagtccgaa ctctgacgtc cgcctcgatg tggcataggc cgtcacgccg
     5041 ttgagtccga tcgccgccag caccagcgcg aacagcccga acagggtcag tagcagcgca
     5101 tagagccgct cctcgtagag gctgtcctcg atcagtcttc gttgcgtctt gacatccgtc
     5161 atgttgaggt cgcggttcac gcgtgcaatc gcctgccgca ccggtccagc catggccgcc
     5221 ggatcgcccg cggtccgcac tgcgaacgcc atccccgtcc acggccggtg atcctgtgcg
     5281 tacggtacga acaccgtagg cggcggcgct gtccgaagac tgttgtactt ggcgtcgcgc
     5341 accaccccaa ccacttgcac ctgccaccgc gccttgtcgt catggtggtc atgccgccac
     5401 aacacctgcc cgattgccgg ccgctcgccg aacagccggc gcgcggcagt ctgattcagc
     5461 accaccacgt tgggcgcggt cgcggtgtcc tggcgcgaaa gagcgcggcc ttgtacgacc
     5521 ggcactccca tcgtctggaa aaattcggga ctaacggtgt tcatccacag attgatccgc
     5581 cggccattct cgatatccgg caaggataca tctgtgctat caaccccgcc accaatgatc
     5641 ggttgcgaca acgacgccgc agaaacaaca cctggtatgg cgcggatcaa ttccagtact
     5701 cgatcgtaga gcggcccagc ctgcgccact tggtagccca actctcccgc atccagacgg
     5761 aatgtcagaa cgcggtcgcg cacgaatccg gcatcgacgt gcgcgagatt gtcgagagtg
     5821 cgaagcagca tcccggcgcc tgccagtaac accaccgaca gggcgacctg gatggcgatc
     5881 agcccccgcc ccatcacggc tccatgccat ccgaccgagc gccgtgcgcc cgctgtgcgg
     5941 ccaccctgct tcagattcgg cgcgatctcg acgcggctcg ccttaagtgc gggaaccagc
     6001 ccgaacagca ccgcgcttgt caccgagacc agcgtagtga acgccagaac cgtcaggtcg
     6061 ggagcgagat ccaatgcgac cgtagcgttg ccggtcaact gcgcagcgat caaccgcgac
     6121 ccggcaaccg ccaggagcag tcccaaagct gctcccgcgg ccgacagcag gaaaccctcc
     6181 gtgagcaact gtcgcacgat gcgcgcgcgc ccagcgccaa gcgacagccg caccgccacc
     6241 tcccgatgcc gcgcagcgga gcgcgcgagt tggagattgg ccacgttgac gcacgcgagg
     6301 agcagcacca gaacgaccat gccgaaaaga atcagcagcg ggcgctgaat gctgtcgcgg
     6361 agctgcgcga gccccttgtc gccgtctaac aggacgacac gcggattgcg ggcctgctcc
     6421 agcgcgggca cgccggaggc cgcgaatgtg ccgcgaaaca gtgccgttag ccttgctgcc
     6481 gcggccttgg tggtcacgcc ggggtgcttc cgtgcgaaaa tctgaaccca tgagaaatcg
     6541 ggcgcggtct tcggcctgcc ggtgcgaagc tcacgaggca tcactgtatc catcgcgttc
     6601 aggggaagga aaagctcggt gggaccgccc cagcccgccg atgcgacgcc gtaaaatcct
     6661 ggcgcagaca cgcccacaac cgtcacgggc actccggcca cgcgcagcac tttgcccaga
     6721 atcgacgcat cgccttcgag cgagtgctgc cagaaatcat aggtgaccac cgccgccgtt
     6781 gcgccgttcc gatcgtcctc ctccgtcaac gggcggccga gcgctatcct ggcccggatt
     6841 gcctcgaaga agttgccggt gacgaaggtg cagtgtgccg gccacatcgt cgtgccgtcg
     6901 ctgactctaa catgtccggg aacgtagaaa ccgaatacgt ccagcatgcc ggcaccggat
     6961 tcccgaaatt gccgcagcgc cgccaacgtg aacgtgctgt tcagccattg gccggatgcc
     7021 gggtccttac ggttaacacc gttctgcgtg ggcattcgga ccggcacatc cgtcttccag
     7081 taggcaacca tgagctgctg cgggtcgctc accggaagag tgcggaccag cacggcgttc
     7141 actacagtga agatcgacgt agtcgcgccg ataccgagtg taagggaaag caccgctacg
     7201 gccgtgaatc caggattggc acgcagaacg cggctggcat actgcaggtc acgcaggata
     7261 gattcgacga atgcgatccc gcgcgcgtcg cggcactctt ctctgacctg ttccgcattg
     7321 ccgaaatcca ggttcacccg gcgccgtgcc tcttcatgcg agagcccggc gcgttccagc
     7381 ttttcggtct cccgttcgat gtgaaattgg agttcgtcct ccaggtcccg ctccatgccg
     7441 ttgcgccgca cgaccgcgcg tagccggtac agaagatcgc ggatcactgt gttctccttc
     7501 aggtcgtctc gagtaccccg cccacgcccg ccaccagcat gcgccagtag cgggtttcct
     7561 gttcgagttg tttccgcccc gcagtagtga gctggtagta cttcgcgcgg cggttatttc
     7621 tggtcacttc ccatttgcct ttgatccagc cgcggcgctc caagcgatgc agcgcagggt
     7681 acaacgatcc ctgttgcacc tgcaggatgt ccttcgacat ctgccggatg cgctccgaga
     7741 tatcccagcc gtgctgtggt tcgagagcaa tgctcttgag aatcagcaga tcaagagtgc
     7801 cttgcggaag gtctatacgt tcagccggca tatgggctac cctgctactt ctacaactga
     7861 gcttactcca actgatgtag aagtacaagg ggagaacggc ccggggtgtc acccgaatcg
     7921 ggatcctcga ctattggcga catctaaatg atgcatcgag ctaaccgcga tagcatcgaa
     7981 tcgggcagag aggaagtatt atgcaggctg cggtcgtttc cgctgtgaac acttcgtggc
     8041 aggtcaaaga agttccgcaa cctcagcccg gacccaatca agtgctcgtg aaaatgcatg
     8101 caagcggcat ctgctacacc gacgtccatc aaactcttgg gcgcttgccc ggaccattcc
     8161 cccgaatcct cggccacgag ccggtcggcg agattattgc cgtggccccc ggcgtcacca
     8221 cgcggaaggt gggagaccgc gtgggcaccg cttggatcca atccacttgc gggcgctgcg
     8281 aatggtgcca gcgtggccgc agaatgttct gtccctacct gaagggtaca ggcgttgagg
     8341 tacagggcgg acacgccgag tacatgctga tgaatactga tgcgacctat ctgattcccg
     8401 gcaaggtttc ctatgagcaa gcagccccca tcttttgcgc aggttacaca gtttatagcg
     8461 ggcttcgctg ggcggatccc aagccacatg agcgtgtggc tgtgctcggg atcggcggac
     8521 ttgggcattt ggccgtgcaa tatgcgaagg ccgcaggctt cgagactatc gcagtttcgc
     8581 attcacccga taaagacaag atgatccgcg atctcggcgc ggatgaggtc gtccgcgacg
     8641 ggaagagcct cgcggcagtt ggaggtgccg atgtaatcct cagcacctca aactcgacca
     8701 agtcgatggt ggacagcctt cagggattgc ggccggatgg ccggcttgtc accatgggcg
     8761 ccgacgtgga gccgctttcg gtctcgctgg cggatctcat cgggaaacgc attcggataa
     8821 ttggcagcca gcagaacggc cccgaatatc tgtacgaagc gcttgatttc gtagcgcaag
     8881 gaaaagtcaa gacgattgtc gagacgtatc cattgacaca ggcagtgaag gcctatgatc
     8941 gggttgccga aggcaaggcc cggttccgcg tggtcctcac aatgtgatcc tcgttccggg
     9001 cagtctgttt cgagcggcta aaccccgcgg gccttagaat gcaaacttca gcccgaagag
     9061 aatcacgcgc gcggcggtga agctggtcgt ctttccgaat gtgctgggca cggaaagatt
     9121 ggcgccaggt ccgcctggat tcgggtggtt gagcatattg aagaactcgc tccggaactg
     9181 gatgtcgtag cgttcggcga accggaagtt tttcatgatg cccgcatcca ggaaccacgc
     9241 gcctggtgtc cacatgctat tgcgcccaac gttcccaagt gtgaacggcg ctggttgccg
     9301 gaacgcgttc gggttgaacc agccttccac gcctggatcg gataccctcc aatcgccaac
     9361 cacatcggcc ctgccgctgg ttggaaaacc gaccaactgc ccactgtagc tgacgtgata
     9421 aggcgtgcca gtgcgcagcg tagcgatgcc cgaaacctgc cagccgccaa tgacatggtt
     9481 gaggggagcc gggacgccgg aaaaaagctt cctgccgcgg ccgaatggga ggttgtacac
     9541 ccagttggtg acggcctggt gccttacgat gccatccatc ggtccccggt ttcgccgggc
     9601 gtcgaacggg taatcgatac cgccgacgct tccccagccg tcggcagcgc ccgcgccatc
     9661 cgagatcgat gacgtgaact gatactcgaa ctggtaggtg aggtcgcccg cctttcgggt
     9721 cgagccgatc tgtaattgat gtgtcaccgc attgccacgc ggatcgatcc atcgaatatc
     9781 gccccaaggc tgaagcggac gcagcgtttg cagggttccg ggtccgaacg cacgaggaag
     9841 atttaaatcg tactcctgcg cggcgaagtg tgtctgattg ccgacatagc ttatgcgcag
     9901 agaggtgttg gggaccagtt cccgctcgat cgtgaagttc cactggaggc tctgcgggtt
     9961 cgggaaatcc cgcggcattc caagaatcga aggactcgac gggatgctcc cgatgcccgg
    10021 aaacggatcg gcgaatgtta cgtaggggtt gttgcccgga ggagagttat agttctcgat
    10081 cagcgcaaac ggggcgaaca gcacctggcg aagaccaagc tggttggaag cctcgtaggc
    10141 gtaatagatc cccgcgccgg aacgaatgac tgtgctactg tcaccgaaag gccgccatgc
    10201 gaaacctacg cgcggctgga agttccgcca gttggtgtcc cgagtaagcg tggtggtccc
    10261 taaaccggcc tgtacactcg tcacgaccgg atagctctct gccaggcgcg taaccacttc
    10321 cggcggaaac tggtcggttc cactataggt caccaaccgg tttgatgccg gatcgtagtt
    10381 cgcctggcgg agtttctttt ccttgaatgg cgtctcaacg tcccagcgga tgccgagatt
    10441 tagtgtgagg ttgcgggtca cctgccagtc gtcctgcaca tagcccccga agcgcttctc
    10501 gttgaaatcg ccgaactgat acgccggacc gcgctggctc tcgttcgcat accccagcag
    10561 gaagtctgcg aaagcgtcgc cggtataacg tccggtgaat ccgaagatgc ccagaccatg
    10621 cggatagatg ccgccccaat atccctggta acggtaataa tatccgccga atttcagttg
    10681 gtgcgtgccc cggatgtaaa ccaggttgtt aaaaatattg atgtctatcc ggttgtcttt
    10741 cgcgtgtgcg ccaaccgtgg aaattccctg gtagccggtg atgtttacgc cgggcagacc
    10801 gccgccgaat cgcccatcgt aatttttgtc gggactccgt ttttccgcgc agcattggcc
    10861 gtcgagttgg ggaaagagcg ttcccggatc gaaatcgttg tgctcattgg tcaggtgata
    10921 gccgtaaggt gtggtaacac ccagacgaac ctcgttggtc aaccgcggtg aaatgatttt
    10981 ggtgtacgcg gcaaagtatt ggcgctggtc gaaaccctgc gtgtaattgc cgaacagttc
    11041 cggtccggtg ccggctcgca gataggggcc gtcgttgaag atcttagcgc cgccgctcag
    11101 gatatcgttc gacgtcggat ggtagtccat cttgatgcct atcctcggcg aatcctgttt
    11161 cgtctggttc ctgccgatga aattgacgcc ggtacccgcc ggtccggtgc cttccaggtt
    11221 cgggtcccgc atgtagccca tgaagaatgt tgccgccggc gagatgcggg aaccaggcat
    11281 ccggttttcc ggaaacggcg cagacgtaag aggatcggtg atggttctga gcccggcgaa
    11341 gtttccctgc ttcatcgaaa cggtcgccat gttgaatgtc cgcgtggccc actcgcgctg
    11401 gctgagctgc tcgaacgatc cgaagtaaaa cagcttgtct ttgatgatgc gtccaccgag
    11461 cgttccgccg aattcattcc ggttatatgg tggcttctcg cggtgatcca gtgtgtactt
    11521 tgcggcgaac tcacggttgc ggttgaacca gaagaggctc ccgtgcaggt cgttggctcc
    11581 cgaccgggtg acgacgatga gttgtgccgt gccgtggctg tattcggccg aggcggtatt
    11641 cgtgataaac cggaactcct cgatcgagtc gaacgttggg tcggcggcgc tgggaccgcc
    11701 attctgcatg tcaaggttca ccgcgccgtc gactaccacg ttaaggcctc cggtaaaaaa
    11761 ccctcccgag acgaagcggc caccccgctg cactcccggt gcgagtgcga tcaatcctcc
    11821 gaggttgtat cttccattaa atggaagttg agtcacttgc gtatgcgtga tgacggaaga
    11881 aagagtcggc ctctcggttt ccagtacgcc cgcacgtgca gttacttcga cccgttccga
    11941 cacaacgccc accttcatct ggatgtcagc gcgggcgaac tgcgcgacag tgacggtcag
    12001 gccgctaact tccacgggcg agaaccccgt catctgggcc gaaagtgtgt agcggccggg
    12061 ggacaggttc gtgaaagtat acgatccagc ttcattcgtc ctggtgacga acttcacacc
    12121 tgtggcctcg ttcgtcagga ctacttccgc gttaggtatc agcgcgcctg cctcatccgt
    12181 aatctttccg gtgaggctgc cgctcacagc cgcctggcct agaagaaccg ccggcaaacc
    12241 caacatcagc gccagcgcga aaagcatccg cgttttacgt tgcagcatgt cgcccccttt
    12301 agtggctaat tcccagacct ctagatgctt atatcacggc aatatgtgct gtcaagaaaa
    12361 accgacgtcc ggatatacaa tggcctgtat cgataaagtg agaaattcaa tgaatatcgg
    12421 tcctaatcgt agagagtttt tgaggagagc cggtcttggc gcagccggtt tcgcagtgcc
    12481 cggcctgtcg cgcggcattg ctcagaaaag caagcgtccg aacttcgtca tcatcttcct
    12541 cgacgatgcg gatgggctga tttccggccc ttcggtaagc ccgattaccc cacacccaat
    12601 gtcgaaggaa tggccgcgcg cggctgcacg ttcaacaatt tttacgtgcc gcaggcggtc
    12661 tgctccgcat cccgttccgc cctccttagc ggatgctatc ctggcaggac aaaagtgttt
    12721 ggcgcacatg gaccgcgggc gcgtgggctc gatccgaaat tcgccaccat gggcaatgtg
    12781 ttccagtccg ccggatataa aacagccgcg ttcgggaaat ggcacatcgg cgatcagccg
    12841 gacacgcgcc ctctccggag gggtttcgat gaatcgtgcg ggctgatgta ttccaacgat
    12901 atgtgggagt tccatccgga aaacccgcaa gcttacagca agtacccgct acagttctgg
    12961 aaaaacggcg agatcacgat tgagcgggtt acaccggaac accagcgcat gctgacgacg
    13021 tggtacaccg ggcatgccgt cgacttcatt cgccggaaca aagagaatcc gtttctgctg
    13081 tacgttcccc actccatgcc gcatgtacca ctgtttgtga gcgacaagtt caaaggcaaa
    13141 tcaggggctg gcctttacgg agacgtatta atggaagtcg actggtcagt aggccagatt
    13201 tcgaaggcgc tgaagtccgc cggagtggaa gagaacaccc tggtgatttt cacttcggac
    13261 aacggtccgt ggacgtcgta tggaaaccac tccggtaaga ctcccttccg ggaagccaag
    13321 gggaccggtt tcgacggggg cacacgcagc ccgtgcatta tgacgtatcc gggccgcatc
    13381 aaagcggggt ccgtttcgaa acggactttc tgctcggtgg acctgctgcc gacacttgcc
    13441 catctggcgg gggcgccact accggaaaat ccgattgacg gccggaacgt gtgggatctg
    13501 atttccggaa agccgggcgc ggtgaatccg cacgaatact atcccttttc taccggcagt
    13561 gtctttgagg gagtaatcag cggggacggc cggtggaagt tgcaccttcc tcaccaatac
    13621 cgggtgctag agaaggcggg tcatgatggt atggctggaa agtaccggca ggagcgcatc
    13681 gagctttctc tcttcgatat ggagaaagat cctctggaga ccaccaatgt gatcgataaa
    13741 ttcccggacg tggccgcgaa aatgaaggga tacgccgaac accaccgcaa gcagttctat
    13801 agctgaggcc gctaggccgc tattttctgc tcgagtattt gttcagcagt gaaatgagcc
    13861 ggctggcgcc cgcgggtacg gcaaggccgg agttgacgac atcgcggttc gcgtaggctt
    13921 tgccgtagat gccctcgttc accgagatat cctgacgcag cgtggcgcct tgcagagata
    13981 caccggcgaa gaggccgcgc gaacgggacc aggtgagtat ttcagcgcgc atcgttgcgt
    14041 ccgtttgtgc cgatgccgtg cggcccacgg gaccggctgc ggccgaggcg tcgccaccca
    14101 ccgtaaactt gctcgagagc agccggtcgg ctccccgttc attcatcacg agcatcacta
    14161 catccgtctc cgacccgccg agttgaaaac cgaaactgcc tccctccacg cgaaccgcac
    14221 ctggagcgga ccagccgact ttgcccgctt tgcggcagga cagaaaaccg cggccgtatt
    14281 tcccgccgat aatgaaggcg gcttttttca aacccggaac cacgacgata cagtgcgacc
    14341 ggtcgaggag atcctgagga atcgctttat ccggcgccgc cataacctct tccagcaccg
    14401 cggcggattc gttcaggcgg ttgagatcat ctttcgatgc cgaaaacagc acggcgttta
    14461 tgcacacgag cagaactggg agtcgcatcc tcataggggg taacctgaac tacatcatcg
    14521 cacgggcgcg gccttgagtc attccggaat ggggccgaga agcg
//
LOCUS       DASTMK010000382         4429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_51296, whole genome shotgun sequence.
ACCESSION   DASTMK010000382 DASTMK010000000
VERSION     DASTMK010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51296"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFQ79_16640"
     CDS             <1..1005
                     /locus_tag="VFQ79_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141352.1"
                     /translation="TQARKRGMGTVYTNADTWSSQQFGTLPHLPFPYQWYERYRALEE
                     SGVEGALESWTPGFKPNFVAEVRAWYSWTEAPPLDHILRQIARRDFGAGSEDLVLDAW
                     KRFSSAVRMNPNTGPSACGYNAVANPLFFKQPAEHIHTMGHSFCDQKEWMEATNTNPY
                     WPYVIRSFLFYPDFTNRVNMAQEYTKPFSLKVFRKYLLAAADEMERGLESYRRAALNA
                     PPSKRANAFREVLLAEQIERTMRSSEAILEFEDLRFRLENTRDKAVCLDLLQRMTEIV
                     ESEMARTEASLEAARRDSRLGYEWENDYIYWPQVLEKKLNLLRLTLNEQLPAYRRRYS
                     "
     gene            complement(1013..1966)
                     /locus_tag="VFQ79_16645"
     CDS             complement(1013..1966)
                     /locus_tag="VFQ79_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0141353.1"
                     /translation="MPRPSKTAFALLLLNQFIWGSSWVAIKWAQQQVGPVVLNLWSLG
                     ISVVVLYPFVRTQLRGKPRRRSLQEYSDFAMMGFAGVSGMALLYAWGTSRTLAANGAL
                     ISTAVPVLTSVLAAAVLGERLTYIRLVSLAIALAGVFTLSDIGWSDADFSRGHLTGNL
                     MLLGGTVSNALYVVYSKKLIAVTEPVLLLFWGQLLGFLGALPFLYFDDFTAAAVAGYT
                     PTTWLSLVFLGVVFYALTMVIFFKLLVYLDAGQIMVATYLQPVLGVLTAAIVLRENIT
                     LPMIGSGVLVLAATILATYGESGRLKPGPLPGVGQREGSPD"
     gene            complement(1995..3002)
                     /locus_tag="VFQ79_16650"
     CDS             complement(1995..3002)
                     /locus_tag="VFQ79_16650"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141354.1"
                     /translation="MDKVRLGVVGCGLFGERHLLALRVVRNAEVTAVFDLDRARARQI
                     AAEFRIPRVCDTMEELCTLPELDAVDVVTSEEAHLQPVLRALAAGKHVFVEKPLAVEL
                     DDCTRMIEAACTAGRILMVGHILRFETKCIMLKDELVSGRLGDIVSIHSWGNRPKTLL
                     PRYGRTHPVLENSIHDIDLMLWYTGKHVRRVRGFGRCITTTKHPDIFWGVLEFEDGAI
                     GIVETIWLLPDAAGIGLDDGVQVVGTAGVGNIQLLPGNYNLLRETGYEVPDISYDPSV
                     AGSVRGALRDELAYFCDCVADNVEPNIITPTEAKRAVRVALALIESAAAEREVELLDW
                     D"
     gene            3342..>4429
                     /locus_tag="VFQ79_16655"
     CDS             3342..>4429
                     /locus_tag="VFQ79_16655"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEU0141355.1"
                     /translation="MNRREFLYLGPGVYALRSPAADPLSRESREPIPEPHFPNRLYQF
                     VWRNWELANSDRMAAVIRASPEAVVDLGSSMGLPKKRHLIDDQLARLYITVIRQNWHL
                     LPESQIIELLGWDRQKFDFILKEDDFLDFKLGTKPRCDELTYQPPSQREKSAAEGIRR
                     TVLESFGRAIDRPGEDLFAFVTDLSKTLYVPARDPAAKPHEDEIDLSRDWAISAGGSL
                     EPAAFRFANWLQSVMNAEIVAGTGLKTIRLEVSPGVPGGREAFRIEVNEREVRILGND
                     DAGVLQGIYLLEDLMEVRDGPFLRRGTIERKTVWNPRYLYSYFALYGDPLIDTERDPF
                     PDAYLEKLARSGINGVWMQAVLNTLAPSL"
BASE COUNT          944 a         1327 c         1310 g          848 t
ORIGIN      
        1 acccaggcgc gcaagcgcgg aatgggcacc gtctatacaa atgcggacac gtggtccagc
       61 cagcagtttg ggacgttgcc tcatctgccg tttccctatc agtggtacga gcggtacagg
      121 gcgctggagg agagcggagt cgagggagcg ctcgagagct ggactcccgg gttcaagccg
      181 aacttcgtgg ccgaggtacg cgcgtggtat agctggaccg aggcgccgcc gctcgatcat
      241 atcctccggc agatcgcccg ccgcgatttt ggtgcgggat cggaggattt ggtcctcgat
      301 gcctggaagc gcttcagctc cgccgtccgc atgaatccca acacgggccc gagcgcgtgc
      361 ggttataatg cggtagcaaa tccgctgttt ttcaagcaac cggcggagca tatccacacc
      421 atgggtcaca gtttttgcga tcaaaaagaa tggatggagg ctaccaacac caacccgtac
      481 tggccttacg taattcgttc gttcctcttt tatccggact tcaccaaccg cgtcaacatg
      541 gcccaggaat atacgaagcc gtttagcctg aaggtgttca ggaagtatct tctagccgcc
      601 gcggacgaga tggagcgagg actggagagt tatcggcgcg ccgcgttgaa cgcgccccct
      661 tcgaagaggg cgaacgcttt tcgcgaagtc ctcctggcag agcagatcga gcgcacgatg
      721 cggagcagcg aggccatttt agagtttgaa gacctgcggt tccggctgga gaacacccgc
      781 gacaaggccg tgtgcctgga cctgctgcag cgcatgacag aaattgtgga gtcggaaatg
      841 gcccgaacgg aagcctccct cgaagccgcc cggcgcgact cccgtctcgg ctacgagtgg
      901 gaaaatgact atatttactg gccgcaggtc ttggagaaga agcttaacct gttgcgcttg
      961 acattgaacg agcagcttcc ggcttaccgt cgtcgctaca gctagcagcg tattagtccg
     1021 gagaaccttc acgctgtccg actcctggca acggacccgg tttcagacgc ccggattcgc
     1081 cataggtcgc gaggatagtc gcggcaagga caagcacgcc gctgccgatc atcggcaagg
     1141 tgatattctc gcgaagcacg atggccgcag ttagcacgcc gaggactggc tgaaggtagg
     1201 tagctaccat gatctgtccg gcgtcaagat acactagtag cttgaaaaag atgaccatcg
     1261 tgagcgcata gaataccacg cccagaaaga cgagtgatag ccaggtggtg ggagtgtagc
     1321 cggcgaccgc ggctgcggta aagtcgtcga agtaaaggaa ggggagggct cccaggaatc
     1381 ccagcaactg gccccagaag aggagaagga caggctctgt cacagcgatc agcttcttgc
     1441 tgtagacgac atatagcgcg ttcgagacgg tgccgccaag cagcatgaga ttgccagtca
     1501 ggtgcccgcg cgaaaagtcg gcgtctgacc agcctatatc cgaaagagtg aatacccccg
     1561 ccagggcgat tgccagactc accagccgaa tgtatgttag ccgctcgcca agaacggctg
     1621 ccgccagcac cgaagtcagg accggtacgg ctgttgagat caatgcgccg tttgcggcca
     1681 gagtccggct ggtgccccac gcatacaaaa gcgccatgcc gctcacgccg gcgaaaccca
     1741 tcatcgcaaa atcagagtac tcttgaaggc tccgccgtct gggttttcca cgcaactgtg
     1801 tacggacaaa agggtagagc acaaccaccg agatacccag gctccagagg ttcaacacaa
     1861 ccggccccac ctgctgctgc gcccacttga tcgcgaccca gctactgccc cagatgaact
     1921 ggttcaacag caggagagcg aaggcggtct tgctaggcct cggcatatat agttgtactg
     1981 gcgacttgcc gcgatcagtc ccagtcgagg agttcgactt cgcgctcggc tgccgccgac
     2041 tcaatcagcg cgagagctac ccggaccgca cgctttgctt cggtcggcgt gatgatgttc
     2101 ggttcgacgt tatcagcgac acaatcgcag aagtaagcca gttcatcgcg cagggcgccc
     2161 cgaaccgatc cggctacgct cggatcgtaa ctgatatccg gaacctcgta gccggtctcg
     2221 cgtagcaggt tgtaattacc ggggagcagt tgtatgttgc cgacacccgc ggtcccgaca
     2281 acctgaacgc catcgtcaag cccgattccg gcggcgtccg gcagcagcca gattgtctca
     2341 actatgccga tcgccccgtc ctcgaattcg agcactcccc aaaagatgtc cgggtgcttg
     2401 gtcgtggtga tgcagcgacc gaaaccgcgg acacgccgca cgtgtttccc tgtgtaccac
     2461 agcatgagat cgatgtcgtg gatagagttc tccaacaccg ggtgagtgcg gccgtagcgc
     2521 ggcagcaaag tcttcggccg gttgccccaa ctatgtatcg agacgatgtc ccccagtcgg
     2581 cccgagacca actcgtcttt cagcatgatg cacttggtct caaatcggag gatgtgcccg
     2641 accatgagaa ttcggcccgc ggtgcaagcc gcctcaatca tcctggtgca gtcgtcgagc
     2701 tccaccgcga gaggtttctc tacaaataca tgtttcccgg ccgccaacgc cctaagtact
     2761 ggctggagat gggcctcctc tgacgtcacc acgtcgacgg cgtcaagttc gggcagcgtg
     2821 cagagttcct ccatggtgtc gcagactcgc ggaatccgaa actcggccgc gatctggcgg
     2881 gcgcgtgcgc ggtccagatc gaaaacggcc gtgacctccg cgttgcgcac aacacgcaga
     2941 gccagaagat gacgctcacc gaacaggcca cagcctacca cgcctagacg caccttatcc
     3001 atccaattcc ctccactacg atcaatccac ctgtcagaat gcgccgccac cctcaacagg
     3061 ggcggcgcat tctaagcacg taattagtat acgcgatgta tgtagtctgc ttgacgtgtg
     3121 cggccttccg tcgcgacgca gtctgctcaa atgtctcgtc agcgccgaaa gaacgaggcc
     3181 agcgcctcgt ggaaaatcag tggacgctgg gattatagga tagtatattc attgtattca
     3241 atgtgtcggc tgtgtcggct ggctggtggc ccgctccggc ggtgttcgcg tcgcgtggcc
     3301 ggcaatggcc ggaacgacat tttgttcgga ggccggcatt tatgaatcga agagaattcc
     3361 tttacctagg cccgggggtg tacgcattgc gcagccccgc cgcggaccct ctgtccaggg
     3421 agagccgcga gccgattccg gagccgcact tcccaaaccg tttgtatcag ttcgtctggc
     3481 gaaactggga gctcgccaat tccgacagga tggccgctgt gatccgcgcc agtccggaag
     3541 cagttgtcga cctcggctca tcgatgggcc tgccgaagaa gcggcatctc atcgacgatc
     3601 agctcgcccg gctgtacatc acggtgatcc gccagaactg gcacctgctg cctgaatcgc
     3661 agatcatcga gctgctgggc tgggaccgac aaaagtttga tttcatcctc aaggaagatg
     3721 attttctcga tttcaagctc gggacgaaac ctcgctgtga cgaactgacc taccagccgc
     3781 cttcgcagcg ggagaaatcc gccgccgaag ggatccgccg gaccgttctg gagtccttcg
     3841 gccgtgcgat cgaccggccc ggggaggatc tgtttgcgtt cgtcacggat ctgagcaaga
     3901 ccctctatgt tccggcgcgg gatccggcgg cgaaaccaca cgaagacgag atcgatttgt
     3961 cgcgggattg ggctatctca gcgggtgggt cgctcgaacc cgcggcgttc cgcttcgcca
     4021 actggctgca atcggttatg aatgccgaga tcgtggccgg aaccggccta aagaccatcc
     4081 gactggaagt cagcccgggc gttcccggcg ggcgcgaagc ctttcgaatc gaggtcaacg
     4141 aacgcgaggt gcgaatcctg ggtaacgacg acgcgggcgt gctgcaaggc atttacctgc
     4201 ttgaggacct catggaggta cgtgacggcc cgtttctgcg ccggggaaca atcgagcgga
     4261 aaacagtgtg gaatccgcgc tacctctact cctattttgc gctgtatggc gacccgctca
     4321 tagacacaga gcgcgacccg tttcccgacg cttacctcga gaagttggct cgctccggca
     4381 tcaacggcgt gtggatgcag gccgtcctca acacgctcgc accttccct
//
LOCUS       DASTMK010000383         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_210382, whole genome shotgun sequence.
ACCESSION   DASTMK010000383 DASTMK010000000
VERSION     DASTMK010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210382"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2195)
                     /locus_tag="VFQ79_16660"
     CDS             complement(<1..2195)
                     /locus_tag="VFQ79_16660"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HEU0141356.1"
                     /translation="MRILLSLLILASGVFGDELLTGYRLQGLRYQGPGEWRQSAEPIW
                     VYEYHTLRFRYRASGLPKSDAAVLTLRPGSVGPVTPGANNIENPFVAGRPVVVVTAPD
                     LISDGAVHTVDIELRGKLRTAQIDQLQFALPDGATLEIEDLQFRGDPAARPCTTAGPP
                     LPEDAVKLPARGPLACAGWPATGLRGRDSIHIEGGGRKGATLYLSMNAQFPGVGKWLV
                     SSQKSWRDTEIKESSETASVLARIHYADGSGEEQFPLLVAERRHAVLNRKAALYALSL
                     DAARPVASIELMDRSPHAQLVLYAAGLSPKSAPSTRAEEEPPPVTGPASGEPETKSTY
                     RVTTLEGKRAPGKAVQAEFREEKQPAGRRLSLAVKNVSSEAQEFNLVFPSVTIRPAAD
                     ASDVWYLFPRQGAVLSREEKPLEAMYSGEFPLQFVDVFAPAANRGTCMILQDPGGRWK
                     KFRLHKTGASVRVEVEYTVRLAPGETFRAPDARIVAHGGDWRQGFETYRQWVATWYKP
                     KGPRPAWLRSAFWARRDYPVGGTGRLFDTRRNRYTYDEFIRDGQLFDGIDFIDISGWA
                     LSETRGRVGDYSIELGGADDLRRNVAAGNKAGIPTGLYFEGYLIDKNSKVGREHGSEW
                     QIIKADGTGQWWQGAVELFACPYAEGWQQFLSQRVAAVAREVGAAAVYLDEFGFGRKR
                     CYSTKHGHPPGEETLCGELAMTSRVRQALDAAGMRDTIIYIEETPPDAAA"
     gene            2345..3958
                     /locus_tag="VFQ79_16665"
     CDS             2345..3958
                     /locus_tag="VFQ79_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0141357.1"
                     /translation="MSVGFRALFENGFSRVTRRGLFHWAGLAAGSGSLLPGAAAAPSK
                     AGALDIGPNIYQSIGVQPVVNCKGTFTIISGSLMLPEVRQAMMEASKHYVHLDELMQG
                     VSQRLAQLTGAEWGIVTNGCASALAHSTAACIAGADPEKLQRLPDLDGLKNEVICPRY
                     SRNVYDHAIRMLGVKMINVNTPEELERALNPRTAMIMILASPAADREPLSTQTVSQIA
                     RKHGVPVLVDAAAEVLTIPNKHLQAGATMVAYSGGKCLRGPQSAGLLLGRKDLVQAAW
                     LHSAPHHAFGRPMKVGKEEIMGMLAAVEQWVKRDYQAEYKVWESWLESISGKVTKVAG
                     VTTQVQAPRGLSNHAPLLHIRWDGTKLGINGRDVEKILIETEPRIILSGARGGSRDAA
                     ESSVTIMPYMMTPADHTVVADRLYAILSKPPAARSAAPAGAAMPNIAGQWVLRMKYFC
                     GTAEHRLFLEQNGTSLTGVHHGEELKGDLRGEVEAGEIRFRSRHRYEGTSLEYDFAGK
                     LAGEQMSGKVGMGEYGLGEWTAERYPYRA"
     gene            3990..>4791
                     /locus_tag="VFQ79_16670"
     CDS             3990..>4791
                     /locus_tag="VFQ79_16670"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0141358.1"
                     /translation="MKLIAFLLLGLFAVQAADLKVVAPPGPPLVGPFSPGIFAGDFLY
                     VSGQGARNAEGKFAETTEQQTTDCLANVRTVIEAAGLTMEHVVYMQVYMKDVTHYDRM
                     NESWRKVFTKNPPARATLGVHRMPTDTPIEINAVAVRDLKMKKIVNPRSFQAASTISA
                     GVLAGNRMYVSGLVGRDPKTGEIPSDPAAQVQLSLDRVKETLAEAGLDFRHVAFVNPY
                     LTDGVPMNVMNKVYAKHFEFGNTPARATIRVTGLPRGANIEFTMIAVRD"
BASE COUNT          925 a         1484 c         1497 g          885 t
ORIGIN      
        1 gcggcggcat ccggcggggt ctcttcgata tagatgatgg tgtcgcgcat gcccgcggca
       61 tccagcgcct gacgcacgcg gctggtcatg gccagttcgc cgcacagcgt ttcctccccg
      121 ggcggatgtc catgcttagt ggaatagcag cgtttgcgtc cgaagccaaa ctcgtccagg
      181 tagacggcgg cggcccccac ctcgcgcgca acggctgcca cgcgctggct gaggaactgc
      241 tgccagccct ccgcgtaggg acacgcgaac aattccacgg cgccctgcca ccattggccg
      301 gtgccgtccg ctttgatgat ctgccattcg ctgccgtgtt cccggccgac cttcgagttc
      361 ttgtcgatca ggtagccctc gaagtacaga ccggtgggga tgcccgcctt gttgcctgcg
      421 gccacgttgc gccgcagatc gtcagcgccg cccagttcga tggaatagtc gcctacgcgg
      481 ccacgcgttt ccgaaagcgc ccaccccgag atatcaatga aatcgatccc gtcgaaaagt
      541 tggccgtcac ggatgaattc gtcgtaggta tagcggttcc ggcgcgtgtc gaagaggcgg
      601 ccggtgccac ccacagggta gtcgcggcgc gcccaaaagg ctgaacggag ccatgcgggc
      661 cgcggcccct tgggcttgta ccaggtcgct acccattggc ggtaagtttc gaaaccctgg
      721 cgccagtcac cgccgtgtgc gacgatccgg gcgtcgggcg cccgaaaggt ttcaccgggc
      781 gccaggcgca cggtgtactc gacctccacc cgcacgctcg cgccggtctt gtgcaaccgg
      841 aattttttcc agcggccgcc ggggtcctgc aagatcatgc aggtgccgcg gttggccgcg
      901 ggggcgaaca cgtccacaaa ctgaagaggg aactcaccgc tgtacatcgc ctcgagcggt
      961 ttttcttcgc gcgagaggac cgcgccctgc cgggggaaca gataccacac gtcggaggcg
     1021 tcggcggccg ggcggatggt cacggaagga aataccaggt tgaactcctg ggcttcgctg
     1081 ctgacgttct tcacggccag cgataacctg cgtccggcgg gctgtttctc ctcccgaaat
     1141 tcggcttgca cggctttgcc cggtgccctt ttgccctcca gggtcgtcac acggtaggtg
     1201 ctcttcgttt ccggctcacc ggaggcgggc cccgtgacgg gaggaggctc ttcctccgcg
     1261 cgagttgaag gcgcactctt cggagaaagc cccgctgcgt aaagcaccag ttgcgcgtgc
     1321 gggctgcggt ccatcaactc gatggaagcg accggccgcg ccgcatcgag gctcagtgca
     1381 tagagcgcgg ctttgcggtt aagcaccgcg tggcggcgct ccgccaccag cagcggaaac
     1441 tgctcttcac ccgagccatc tgcgtaatgg atccgcgcca gcacggatgc tgtctcgctg
     1501 gattccttga tttccgtgtc gcgccagctc ttttgggaac tcaccagcca cttccccacc
     1561 ccggggaact gcgcattcat gctgagatag agcgtcgcgc ccttgcgccc gccgccctcg
     1621 atgtgaatgc tgtcgcggcc gcgcaagccg gttgccggcc aacccgcgca cgcgagcgga
     1681 ccgcgggccg gcagcttcac cgcgtcctcg ggcagcggcg gtccggccgt agtgcacggc
     1741 cgcgcggccg gatcaccgcg aaattgcagg tcttcgatct ccagggtggc gccatccggc
     1801 agcgcgaact gcaactggtc gatctgcgcc gtacggagct taccgcgcaa ctcgatatcg
     1861 acggtgtgaa cggcgccgtc cgatatcagg tccggcgcgg taaccaccac tacggggcgg
     1921 cccgccacaa aggggttctc gatattgttg gcgccgggag tgaccggtcc gacactgccg
     1981 ggacgcaacg tcagcacggc ggcatcggat ttcggcagcc ccgaagcccg atagcgaaag
     2041 cgcagcgtgt gatactcgta aacccagatg ggttcagccg attgccgcca ctcgccgggt
     2101 ccctgatacc tcagcccttg caggcgatag cccgtaagca gttcatcgcc gaagacgcca
     2161 ctggccagta tcaacaatga aagtaggatt ctcatggttt accagaggaa cttgatgccg
     2221 aattgcagtt ggagcccgcg ttcataatca tcagggtaac gccggtgaaa tgagggtagg
     2281 acagcagcct cctatgttgc aattcttagc cagtggcaat acgctggagt tcctggaggc
     2341 tgaaatgtct gtcggatttc gtgcgctgtt cgaaaatggc ttcagcaggg taacgcggcg
     2401 aggattgttt cactgggccg gtttggcggc cggttccggt tctctgctgc cgggtgcggc
     2461 cgcggcgccg tcgaaggcgg gcgctctcga catcgggccg aacatctatc aatccatcgg
     2521 cgtccagccg gtggtcaact gtaaagggac gttcaccatc attagcggct cgctgatgtt
     2581 gcccgaggtg cggcaggcga tgatggaggc gagcaagcac tacgttcatc tcgacgagct
     2641 catgcaggga gtcagccagc gtcttgcgca actgacaggc gccgaatggg gcatcgtaac
     2701 caacggctgc gcgtcggctc tcgcgcattc cactgccgcc tgcattgccg gcgccgatcc
     2761 ggagaaactg caacgcctgc cggatctcga cgggctcaag aacgaggtga tttgcccgcg
     2821 ctattcgcgc aacgtctacg atcacgcgat ccgcatgttg ggcgtgaaga tgatcaacgt
     2881 aaacacgcct gaggaactgg agcgggcgct caatccccgc accgcgatga tcatgatcct
     2941 cgccagccct gcggcagacc gcgagccact ctcaacccaa accgtctcac aaattgcccg
     3001 caaacacgga gtgcctgtgc tggtggatgc cgccgccgaa gtgctgacca ttccgaataa
     3061 gcacctccag gccggtgcaa ccatggtggc ttacagcgga ggcaagtgcc tgcgagggcc
     3121 gcaatcggcg ggcctgctgc tgggccggaa agacctggta caggccgcct ggctgcacag
     3181 cgcgccgcat cacgctttcg gccgtccaat gaaggttggc aaggaagaga tcatgggcat
     3241 gctggcggcc gtggaacagt gggtgaagcg cgattaccag gcggaataca aagtgtggga
     3301 gtcgtggctc gagtctatat cgggcaaggt gacgaaagtg gccggcgtca ccacacaggt
     3361 gcaggctcca cgaggtttgt cgaaccacgc gccgctactg cacattcgct gggacggcac
     3421 gaagctgggc atcaacggcc gcgatgtgga aaagatcctg atcgaaaccg agccccgcat
     3481 cattctcagc ggtgccaggg gcggcagccg cgacgcggcg gaaagctcgg tcaccatcat
     3541 gccttacatg atgacgccgg cggaccacac ggttgtggcc gatcgcctct atgcgattct
     3601 gtcgaagccg cccgccgccc ggagcgccgc gccggcaggt gcagccatgc cgaatatcgc
     3661 gggacagtgg gttctacgca tgaaatactt ttgcgggacg gcggaacatc gcctgttcct
     3721 ggagcagaac ggaacatcgc tcaccggcgt acatcatggc gaagaattga aaggcgatct
     3781 tcgtggtgaa gtggaagccg gcgagatccg tttccgcagc cgtcaccgtt atgagggcac
     3841 atcactcgaa tatgactttg ccggcaaact cgcgggcgag cagatgtcgg gcaaagtcgg
     3901 tatgggcgaa tacggattag gggaatggac agcagaacgc tacccctatc gggcatagta
     3961 tccatcgatc tctttactgg aggaatattt tgaagctcat cgcttttctc ttgctaggtt
     4021 tgttcgcagt tcaggcagcc gatttgaaag ttgttgcacc gcccggtcca ccgttggtcg
     4081 ggccattcag ccccggcatc ttcgccggcg attttctgta tgtctccggc cagggcgcaa
     4141 gaaacgccga gggcaagttc gccgaaacga ccgagcagca gactaccgat tgcctcgcaa
     4201 atgtgcgaac ggtgatcgaa gccgcaggac tgacgatgga acacgtcgtc tatatgcagg
     4261 tctatatgaa ggatgtcacc cactacgacc gcatgaacga atcctggcgc aaggtgttta
     4321 cgaagaatcc cccggcccgc gcgacgctcg gagttcatcg catgccgacg gacaccccga
     4381 tcgagatcaa cgccgttgcc gtgcgcgatt tgaagatgaa gaagatcgtg aatccaagga
     4441 gcttccaggc ggcctccacc atctccgccg gcgtcctggc aggcaacagg atgtacgttt
     4501 cgggcctggt gggacgcgat ccgaaaacgg gcgagatccc gagcgacccg gccgcgcagg
     4561 ttcaactttc gctcgaccgg gtgaaagaga ctcttgccga agcgggtctc gattttcgcc
     4621 acgtggcttt cgtcaacccg tacctgaccg atggcgttcc gatgaacgtg atgaacaagg
     4681 tatatgcgaa gcactttgaa ttcggcaaca caccggcgcg tgcgacgatt cgagtgaccg
     4741 gcctccctcg cggagccaat atcgaattca ccatgatcgc cgttcgcgat c
//
LOCUS       DASTMK010000384         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_20812, whole genome shotgun sequence.
ACCESSION   DASTMK010000384 DASTMK010000000
VERSION     DASTMK010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20812"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VFQ79_16675"
     CDS             complement(<1..1157)
                     /locus_tag="VFQ79_16675"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141359.1"
                     /translation="MIGRIVYSLAGLLWLTASAMGQASEPVRYTLRFPQPETHYVEVE
                     ASFPVDRQERIEIVMPVWTPGSYLVRDYSRHVEGVAASAGDRALKISKTRKNRWTVET
                     AGSARVDVRYRVYGFELSVQTNWIGSEFAMLNGAATFLSLAEKTPRPHEITVGLPPSW
                     KTVATGLAEAPGGENHRFVAPDYDTVVDSPIVAGNPTIHKFEVGGKPHYLFNFGGDRF
                     WDGEAAARDVRRIVESSFRLWGSLPYDKYVFLNVIAEASGGLEHKNSTLLLTSRWAYR
                     IRETGTDRPNYINWLDLVAHEFFHVWNVKRLRPVELGPFDYENEVYTKSLWVAEGLTS
                     YYAPLIVKRAGLISTTQFLRLLSRRIETLETTPGRLVQPVETSSYDAWIHL"
     gene            1243..2346
                     /locus_tag="VFQ79_16680"
     CDS             1243..2346
                     /locus_tag="VFQ79_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141360.1"
                     /translation="MLDLDLGHIPAPPAKKDYAIGVIGAGFIVKDIQLVAYRQAGYNV
                     QAIASDVPENSRAVAAAHGIPKIYDSPADLLADKSIEILDIAVPPHAQLAVLREAVRH
                     SGHIKGILAQKPLAMNYREAKQAAEMCSEAGITLAVNQNMRYDQSIRALKTVLDRGYL
                     GEPVLATIEMRAIPHWQPWIREYGRLTMLVMSIHHLDTFRYLFGDPESVYVSARPDPR
                     TAFPHRDGIVLYILEYANGLRAAAWDDVWTGPAREGTAPDNYIKWRVEGMDGMAQGTL
                     GWPSYPNAAPSTIDFTTKLQPQCWFSPRWKEVWFPDAFAGTMGQLLQAVSDGTEPAIG
                     ARDNLHTMALVDACYKSLEEHRPVRITEILEES"
     gene            2343..3197
                     /locus_tag="VFQ79_16685"
     CDS             2343..3197
                     /locus_tag="VFQ79_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0141361.1"
                     /translation="MNLGLINSAWLGSPVGTAEGIRKTKEIGFDTIDISADPMEIGIQ
                     ERRLIKDTCREMELPVISVCCCALGIADFSAPVRQFHFDRAKAHLDFVYELEARNLLL
                     VLGEYIWQQEVIQPEDQWAWAVDRVRGLGEYAKTLGLEIAIELEPFRLSIVNSVASMD
                     RLLKGVGLPEVVKANLDVSHLALVHTPPEALSQLKGRASHVHFSDCDGKVHGDLPPGR
                     GVVEFLPYLQAIRGIGFDGTLSIELEYSPEPEKIVDWVTEAYRETDRLMREAGITRGE
                     SRTSATVS"
     gene            complement(3235..3414)
                     /locus_tag="VFQ79_16690"
     CDS             complement(3235..3414)
                     /locus_tag="VFQ79_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141362.1"
                     /translation="MYKKVGAVNPLAIELYRAFENGKSVLEIARETGIPVVRVEQRLR
                     AAAAFLARKKSAEVA"
     gene            3764..>3987
                     /locus_tag="VFQ79_16695"
     CDS             3764..>3987
                     /locus_tag="VFQ79_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0141363.1"
                     /translation="MKRRILFLAVACASLSWSAIEPSNRPERLEWFRDQGFGLFIHWS
                     VDSQIGSVISHSMAGASEDYLRRYVSELPKT"
BASE COUNT          828 a         1160 c         1199 g          800 t
ORIGIN      
        1 taaagatgaa tccaggcgtc ataggaagac gtctcgacgg gctggaccag cctgccgggc
       61 gtcgtttcca gcgtttcaat acggcgggac agcaggcgca ggaattgcgt tgtgctgatc
      121 aggccggcgc gcttaacgat caggggcgcg tagtaactgg taaggccctc ggctacccaa
      181 agcgatttcg tgtagacctc gttttcgtag tcaaatggcc cgagctcaac cgggcgcagc
      241 cgtttcacgt tccagacgtg gaagaactcg tgagcaacca ggtccagcca gttgatgtag
      301 ttcggccggt ccgtgccggt ttcccggatg cggtaggccc agcggctggt caacaacaga
      361 gttgagttct tatgttcgag accgccggac gcttccgcga tcacattgag aaagacgtat
      421 ttgtcgtagg gtaaggaacc ccacagccgg aaactgcttt ccacgatgcg gcggacgtcc
      481 ctggcggcgg cttcaccatc ccagaaacgg tcaccgccga aattgaacag gtagtgcggc
      541 ttgcctccaa cttcgaattt gtggatggtg ggattgcccg cgacgatggg cgaatcgaca
      601 accgtatcgt agtccggcgc gacgaagcga tgattctccc cgcccggcgc ctcggcaaga
      661 ccggtggcga ccgttttcca ggaaggcggc aatccgacgg tgatctcgtg cgggcgcgga
      721 gtcttttcgg ccagcgacag gaacgttgct gccccgttca gcatggcaaa ttcactgccg
      781 atccagttgg tctgtacgct gagttcgaat ccgtagacgc gataccgcac atccacgcga
      841 gcgctcccgg ctgtttccac ggtccaccgg ttcttgcgcg ttttagaaat tttgagagcg
      901 cggtctcctg cggacgccgc aacgccttcc acatggcgcg agtagtcgcg taccaggtag
      961 gatccgggcg tccagaccgg catcacgatc tcgatccgtt cctggcggtc caccggaaag
     1021 gaggcctcca cctcgacgta gtgcgtttcg ggctgtggaa accggagagt atagcggacc
     1081 ggttcgctgg cctggcccat agccgaagcg gtgagccaga gcaatccggc gagagagtaa
     1141 acgatacggc cgatcataag ttaatgaagt catggtacac agaagggccg tggatttgac
     1201 accgcaaaat cggcaatcgg ataatgtgct cggcttatac acatgctcga tctcgatctt
     1261 ggacatattc ccgctccacc ggcgaagaag gattacgcca tcggcgtgat cggagcggga
     1321 ttcatcgtaa aagatataca actggttgct taccggcagg ccggctacaa cgtgcaggcg
     1381 atcgcctccg acgttccgga gaactcgcgg gccgtagcag cggcccatgg aattccgaag
     1441 atctacgact cgcccgcgga cctgctcgcc gacaagtcca tcgaaattct cgatatcgcc
     1501 gtgccgccgc acgcgcagct tgcggtcctg agagaggccg tcaggcactc cggccacatt
     1561 aaaggcatcc tggcgcaaaa accactggca atgaactata gggaggcgaa gcaggccgcg
     1621 gaaatgtgca gcgaggccgg cattacgctc gcggtgaacc agaatatgcg gtacgaccaa
     1681 tcgattcgcg cgctgaaaac ggtgctggat cgcgggtatc ttggggaacc ggtgctggcc
     1741 accatcgaga tgcgggccat tccacactgg cagccctgga tccgcgaata cggccggctc
     1801 acgatgctgg tgatgagcat ccaccacctc gacaccttcc gctacctttt cggcgacccg
     1861 gagtcggtgt atgtgagcgc ccgtcccgat ccgcgcactg cattccccca tcgcgatgga
     1921 attgttttgt acatcctgga gtatgcgaac gggctgcgcg cagcggcttg ggatgatgtc
     1981 tggacgggac cggcccgcga aggtaccgcg ccggacaact acatcaagtg gcgcgtagaa
     2041 ggcatggacg gaatggcgca gggcacgctc ggctggcctt cctatccgaa cgccgcgcca
     2101 agcaccatcg atttcacgac gaagttgcag ccacagtgct ggttttcgcc gcggtggaag
     2161 gaagtgtggt ttcccgacgc tttcgccgga accatgggac aactgcttca agccgtgtcc
     2221 gatggcaccg agccggccat tggcgcgcgc gacaatcttc atacgatggc cctggtggac
     2281 gcttgttata aatcgctcga ggaacaccgc cccgtgcgga tcaccgaaat actggaggaa
     2341 tcatgaatct cggactcatc aattcggcct ggctcggttc acccgtgggg accgcggaag
     2401 gcattcgaaa gacgaaggag atcggcttcg acaccatcga catctccgcc gacccgatgg
     2461 agatcggcat ccaggaacgc agactgatca aggacacctg ccgggaaatg gaactgccgg
     2521 tgatttccgt ctgctgctgc gcgctgggga tcgcggattt cagcgccccg gtgcggcagt
     2581 ttcacttcga ccgtgcgaag gcgcatctcg acttcgtgta cgaactggaa gcccgcaacc
     2641 ttctgctggt tctgggagag tacatctggc agcaggaagt aattcaaccg gaagaccagt
     2701 gggcctgggc cgtggaccgt gttcgcggac ttggagagta tgcgaaaacg ctcggcctgg
     2761 agatcgccat cgaactcgaa cccttccggc tgtcgattgt gaactcggtc gcttcgatgg
     2821 accggttgct gaaaggtgtc ggcctgccgg aggtcgtgaa agcaaacctg gacgtttccc
     2881 atctcgcgct ggtccacacg ccacctgaag cgttatcgca gcttaagggc cgcgccagtc
     2941 acgtacattt ctccgattgc gacggaaagg tgcacggcga cctgccgccc gggcggggcg
     3001 ttgtggagtt cctgccttat ctccaagcca tccgcggcat cggattcgat ggaaccttga
     3061 gcattgaact tgaatattcc ccggagccgg agaagattgt cgactgggtg acggaagctt
     3121 accgggagac agaccggctg atgcgcgaag cgggcatcac gcgcggagaa agccgcacgt
     3181 cagccactgt gtcctagccg ggcggagaca gattccgagc gagaacctgc gatttcaggc
     3241 aacttcggca gactttttcc gggcaaggaa agccgccgcg gcacgcaaac gttgttcgac
     3301 gcgcacgacg ggaatacccg tctctcgagc gatttccaga accgatttcc cattctcgaa
     3361 tgctctatag agctcaatag ccagcggatt cacggcaccc acctttttgt acatctattt
     3421 gattggacgg atggcggctg ccgggagttc ccgccgccaa ctttcccagt tcacacaaac
     3481 agttattctc cgtcaaccgg caccgatcga aaatacagga tttgctgtat ttacgcggtg
     3541 gacacgcaac gataggcttt aggcaaagac aggttggtct cgattttgag ctgcgagtcg
     3601 gaatgcaggg cgtgagacgt ttacgccctg cacgttagct tttaagagcg cattcttgtc
     3661 tacgggttta aggtggagct cggtccttca tcctgcaacc cgcccgattc cgccgggcgg
     3721 gtttcttttc cccgcgctgt gccgctacaa tctgcaaaga cctatgaagc ggcggatact
     3781 gtttctggcc gtagcctgtg cttccctctc gtggtccgcg atagaacctt cgaaccggcc
     3841 cgagcgactg gagtggttcc gggatcaggg cttcggcctg ttcattcatt ggagcgtcga
     3901 tagccagatc ggctccgtta tcagccattc catggcgggc gcgtccgaag actatctccg
     3961 gcgctacgtc tcagagttgc cgaaaac
//
LOCUS       DASTMK010000385        11638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_338811, whole genome shotgun sequence.
ACCESSION   DASTMK010000385 DASTMK010000000
VERSION     DASTMK010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338811"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..184)
                     /locus_tag="VFQ79_16700"
     CDS             complement(59..184)
                     /locus_tag="VFQ79_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141364.1"
                     /translation="MPDLLITITVLLLSFIAALGIQFGALKLLLRSMCAKSPAGR"
     gene            complement(221..2644)
                     /locus_tag="VFQ79_16705"
     CDS             complement(221..2644)
                     /locus_tag="VFQ79_16705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0141365.1"
                     /translation="MVWRGFRTVVSELRLKRHKSQPDNRYSSGTWRAARALRDFSERL
                     PLAQKAAVILFLLASGGIIALGTFFWFLGRTADDSRFEVLAARQRMLVQEMYTYALGA
                     RIEEPGIEDRLLSIINEFDSSLWSFEMGGGALGVHIPPAPPEFHPALMRIKRTWVRLR
                     GPLLLLAEEPRNTPELRGAFKAAQRGLPQLAAGTTGLAALFEERKQRLRANMLTVLFS
                     ICFVNLLFVVAALRFTKRQIAAPIAEVTHAARLFQTGNFSARVRVRAKNELAELAETF
                     NRMAANVAWLVARVQASEARFRGLFENVPEGVCKTTTEGRITAANPALLSMLGLEKEE
                     DLFLDTLNSYVAGQAKTPKEEAFPGAAQTRNLELQLRRTDGRTITVLANTCAVRDESN
                     NIIGYQTTLTDISDRKDLEQQLRQSQKMEAVGRLAGGVAHDFNNLLTVILGYSEFALE
                     SLPKGDPVRDSINEICGAAKRAERLTQQLLAFSRKQVARATVLDLNSVIHGMETMLRR
                     LIGEHIQLHLALHPNLWPIYADHGQMEQVVMNLALNARDAMPTGGVLRIETANVYLDE
                     STIGQNSLQLEPGPCVILSVADDGAGMTDEVLSHLFEPFFSTKEKGKGTGMGLSMVYG
                     IVRQSGGDVRVGSQPGMGSVFQIMLKRAGRVETSSLPEPEETSPGQAHGETILLVEDE
                     AGVRQFAAEALRRRGFKVLEATDGREGLRTFERHRAEIDLVLTDVVMPAMTGRELAQI
                     MRAKDPSLPVLYMSGYTDDIILSYGLDGAEIPLLSKPFTADVLTRRVQQTLAASGSRS
                     SSRFSTNQH"
     gene            complement(2751..4298)
                     /locus_tag="VFQ79_16710"
     CDS             complement(2751..4298)
                     /locus_tag="VFQ79_16710"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU0141366.1"
                     /translation="MAMDRLEELRRRKAAAETGGGPERRERQHREGKLTARERVELLL
                     DEGTFEELDKLVRHRCRDFGMEKHVVDGDGFVTGYGHVNGRPVCVFAQDFTVFGGSLS
                     EANAQKIVKVMDLALRNGCPIIGLNDSGGARIQEGVLSLAGYADIFLRNTLASGVVPQ
                     ISAIMGPCAGGAVYSPALTDFVFMVDGASHMFITGPDVIKTVTHEDVSKEELGGGMTH
                     NQKSGVAHFLASGDAHCLRMVRRLLSFLPQNNLDDAPLRATSDPADREDPQLDSVVPE
                     QPDVPYDMRGVILRVVDDGDFFEVQEHFAKNIVIGFAHLDGRPAGIVANQPAHLAGCL
                     DIDSSVKAARFVRFCDAFNIPIITFEDVPGFLPGTNQEFGGIIRHGAKLLYAYAEATV
                     PKITVITRKAYGGAYCVMGSKHLRTDMNFAYPTAEIAVMGPEGAVNIVYRRELAGADG
                     ADEARKAKIQEFRDRFANPFIAAERGFVDDVIEPRQTRPKLIRALRLLENKTDSSPRK
                     KHGNIPL"
     gene            complement(4408..5337)
                     /locus_tag="VFQ79_16715"
     CDS             complement(4408..5337)
                     /locus_tag="VFQ79_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0141367.1"
                     /translation="MRLAGQGQDQAPAPAEETPVFRTGVTQVRVDVQVTDRDGNVITG
                     LTRDDFAVFDENVPQPVAYFGQDRDPLSLLLLLDVSGSMRRYLDQMASTAEKALRYLR
                     VGDRVGVMLFSRQTEVTQPFTGDLVQVAEKIRDSSNEHDLGAGTAINESILDGVSYLA
                     RYKESEFGNRQDRRAVLILTDNLSLNYQTPDSKVTGELLREDTVLNAIVVGKAERPGA
                     PRPGVYQNPDFTPADVFKLAEQTGGEAVKVNRADQTFPEMMERIRTRYTLAYNAPEAK
                     SGTFRQIRVELTPAARQRYSGAVVRARSGYYVR"
     gene            5351..7252
                     /gene="selB"
                     /locus_tag="VFQ79_16720"
     CDS             5351..7252
                     /gene="selB"
                     /locus_tag="VFQ79_16720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEU0141368.1"
                     /translation="MRIQRMKNLTIGTAGHIDHGKTALVRALTGVDTDRLAEEKRRGI
                     SIDLGFAHMDLSSGLRIGFVDVPGHERFVKNMLAGVAGIDMVLFVIAADESIKPQTRE
                     HFDICRLLDVGKGILVLTKSDLVDPDILELVKLEAEDFVRGSFLEGAPIVAVSSTTGE
                     GLEQLRAEIGQMAASIPPRNAGGLFRLPIDRSFSMKGFGAVVTGTLIAGSVRTEQEVE
                     VQPSGQRFRVRGIQVHGEAVESAAAGQRTALNLAGADHRHLARGMMLTVPGLLRPTTR
                     LDCLLYLLPLAKPLKHGAPVHFHAGTAEVEGEIRMLDRLSALAPGESAFLRILLRDPV
                     LLLPGDRYIVRMFSPVVTIGGGRVIDIDVPRRISRAGAFRRMQSLSNADDATRVSTLV
                     RESACGLSLAELVARTGLPLASLETLARRDEFVAPGGWLVDRDWFNAASAGLSRILED
                     FHRANPLLPGIPKQDLRGRQLPDCPPPLFDELVRFNKGLVADGEFVRSATHRITLQEN
                     EAEALERMERLFRDAGLTVPATKDVLARSGLDAARSRRLLQMLLRGGKLVRVGEDLVY
                     HQTALEGLKSLLAGHVGERFAVPEFKDWTGISRKYAIPLLEYLDREGVTRREGNARVV
                     LSALPARKA"
     gene            7303..8301
                     /locus_tag="VFQ79_16725"
     CDS             7303..8301
                     /locus_tag="VFQ79_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0141369.1"
                     /translation="MLFPEFARPTLIGIARQAAGSELLEAKARVQYFELPTRRFLSPV
                     RSRRVPFCWAINPYRGCEFGCRYCYARYTHEFMELREISQFEEMIYAKQFDKAAFRQE
                     LRSTARQESIAIGTGTDPYQPAERRFGVTRQILEVLAGEEGRRISLVTKSDLVARDAL
                     LFQTIAQSNVLHVNITITTLDERLARLLETWAPRPALRVAAVRTLVEAGIRVGVLPNP
                     IVPLITDSEENLDSVAAAAAQAGAESFGGGVLFLKPCSRRVFFPFLAEHFPHLLRRYR
                     ERYEESAFLHGSYPESIRERVRRVRQRHGLVGGGANYEPELWEGEPQLALFDDSAL"
     gene            8480..10276
                     /locus_tag="VFQ79_16730"
     CDS             8480..10276
                     /locus_tag="VFQ79_16730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0141370.1"
                     /translation="MAHPGGITQGPHNRARIVADMLQRSFAGPIANVFAIVVFLFGTD
                     LLQQHPFVTSLFLGILTLRLLGRAVFTCLRRKRMPAREPPLWLIGFSAYALSVPTGCY
                     AAFVLHSYGYEDWNTVLMYVFAMVCAISGTTALAPDLRLGLGYQASLLSPVVIASLAA
                     DGRRCWVGAIAAILFAAYAVMQAVRQNTEYRNAQAASDALRCRAEELEVAQAAAAAAS
                     EAKSRFLANMSHEIRTPMNGVLGMLELVLRSDLSSCQRQDLDCARDSAQSLLVLLNQI
                     LDHAKAEAGRLELEQTDFSVREVVCSAAVPFRFQAAEKEIALSCTVEPSVPQCLRGDP
                     TRLRQVIVNLVANALKFTPSGSIKVAVRVESVTGPDVLLGFRVADSGPGIPTDKQETI
                     FDAFSQADSSITRRFGGTGLGLAICRDLVELMGGQIRVESQPGRGSVFSFTAMFTMSS
                     SEKRETEPARAEPRVCNPLRVLIAEDNVVNQKILMRILQTGGHSVVLAEDGLQAVARF
                     SEDWFDVVLMDVQMPRMDGLQATRHIRAAAGRDVPIIGVTAGATPAEVAACFEAGMDS
                     CITKPINISKLQELLARVRAGNLPEGQPSRCG"
     gene            10359..10688
                     /locus_tag="VFQ79_16735"
     CDS             10359..10688
                     /locus_tag="VFQ79_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141371.1"
                     /translation="MGKPSDLIQGTLDLLILKTLSLEPMHGWGIAQRVEQASRDALQI
                     QQGSLYPALHRLEQQGWIRSEWRETENRRRAKYYLLTPAGKRQYAKELANWQRLSQAI
                     GLVLEMA"
     gene            10688..>11638
                     /locus_tag="VFQ79_16740"
     CDS             10688..>11638
                     /locus_tag="VFQ79_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141372.1"
                     /translation="MRWLDRLRLRFRSLLRRGRVERELDQELSFHIEELTRRQIERGI
                     TAEEARTAALRQFGGVAQFQEACRDQRRTRWIEDFFHDLRYAVRLFAKSPGFTVVALF
                     SLALGIGANTAVFSLLETIVLRELPVREPGALVQIVGRDGRGAQGSFSYPSYLWLREN
                     APMFSHVFTWRFTNVWAGQGDSMEWIAVDKVSEDYFAGLGVSATLGRTLGDEDGQPAV
                     AVLSHKWWKVRFQADPDVVGRELRLGGIQFTIAGVMPAGFSGAEVGKAADVYIPLSAE
                     RLLAPGSEILLRRNASWLPLMGRLRGGLPRQQATAEFEPVW"
BASE COUNT         2213 a         3462 c         3554 g         2409 t
ORIGIN      
        1 tggtccgtta attcaggtct ccacctctaa cgggccgatc cgcatcgaga agctgtaatc
       61 agcggccggc tggtgacttg gcgcacatgg atcgtaagag aagtttgagg gcgccgaact
      121 gaattcccaa ggccgcaatg aagctgagca gcagaaccgt tatcgtgatc agaagatcgg
      181 gcatattgcg ctccatatat cggctgaatt catcaggact ttagtgttga tttgtcgaga
      241 atcgcgagga agatctggac ccggaggcgg cgagcgtctg ctgaacccgc cgcgtgagga
      301 catcggctgt aaaaggcttc gacagcagcg gaatctccgc gccgtcgaga ccatagctca
      361 ggatgatgtc gtcggtgtag ccggacatat agagtacggg cagtgaaggg tccttggctc
      421 tcataatctg tgccagttcc ctgccggtca tggcaggcat caccacgtcg gtcagaacga
      481 gatcgatctc cgcgcgatgc cgctcaaaag tacgaagacc ttcccggccg tcggtagcct
      541 ccaatacttt aaaaccacgg cgccggagcg cttccgctgc gaattgccgc acacccgctt
      601 cgtcctccac caggaggatc gtttcgccgt gggcctggcc cggcgaggtc tcctccggct
      661 ccggtagcga actggtctcc acgcggcccg cccgcttcaa cataatctga aacacgcttc
      721 ccataccggg ctggctgccg acgcgaacat cgcccccgga ctgccgcaca atgccataaa
      781 ccatggagag ccccatgccc gtgcctttgc ctttttcctt cgtgctgaag aacggctcaa
      841 aaagatgcga cagaacctcg tccgtcatac ccgccccgtc gtccgcgacg gagaggatca
      901 cgcaaggtcc cggctccaac tgaagggagt tctgcccgat ggtggactcg tcgaggtaaa
      961 cgttggcggt ctctattctc agcacacccc ccgtgggcat ggcatctcgc gcattcagcg
     1021 ccagattcat tacgacctgc tccatctggc cgtggtcggc ataaatcggc cagaggtttg
     1081 gatgcagcgc gagatgaagc tgaatgtgct ctccgatgag cctgcgcagc atggtttcca
     1141 ttccgtggat gacggaattc aagtcgagga cggtcgcgcg cgccacctgc ttccggctaa
     1201 acgccagcaa ttgctgcgtg aggcgttccg cgcgctttgc cgcaccgcag atctcattga
     1261 tgctgtcccg aaccggatca ccctttggaa gcgactccaa cgcaaattcg ctatagccca
     1321 ggatcacggt cagaaggtta ttgaagtcat gggccacacc gccggcgagc ctgccaacgg
     1381 cttccatctt ctgtgactgg cgcaactgct gctccagatc tttgcgatcg ctgatatccg
     1441 tcagagtggt ctggtagcca atgatgttgt tgctttcatc gcggacggca cacgtgtttg
     1501 ccagcacggt gatggttcgc ccgtcggtgc gccgcaactg aagttcgaga ttgcgcgtct
     1561 gggccgctcc gggaaaagct tcctctttgg gtgtcttcgc ctgtccggca acgtaagaat
     1621 tcagggtgtc gagaaagaga tcctcttcct tttcgagacc gagcatcgac aacagagccg
     1681 gattcgccgc agtgatccgc ccttccgtcg tggtcttgca taccccctcg ggaacattct
     1741 cgaacaggcc gcgaaacctc gcctcggagg cctgcactct ggctaccagc catgccacat
     1801 tggctgccat ccggttgaaa gtctccgcca gttccgccag ctcgttcttt gccctcacgc
     1861 gcacgcgcgc ggagaagttg ccggtctgaa ataagcgggc ggcgtgcgtc acctcggcga
     1921 taggagcggc gatctgacgc ttcgtgaatc gcagtgccgc cacaacaaac agcaggttca
     1981 cgaaacagat ggaaaaaagc acagtcagca tgttggcccg cagacgctgt ttgcgttctt
     2041 caaagagtgc cgcgagcccc gttgtgccgg cagcgagctg gggcaaacct ctttgagccg
     2101 ctttgaacgc accgcggagc tccggcgtgt tcctgggttc ttcggcaagg aggaggagcg
     2161 ggcccctgag ccgtacccag gttcgcttga tgcgcatgag cgccggatgg aattccggcg
     2221 gcgcgggggg gatatgaacc ccgagagctc cacctcccat ctcgaaactc cagagtgagc
     2281 tgtcaaactc gtttatgatc gagagcagcc ggtcctctat tccaggctct tcaatccgcg
     2341 ctcccagcgc gtacgtgtac atctcctgca ccagcatgcg ctgccgcgct gccagcacct
     2401 caaaccggct atcgtcggcg gtgcggccca gaaaccagaa gaaagtcccc agggctataa
     2461 tgccgccgga agccagcaga aacagaatta cggctgcttt ttgagctaat ggaaggcgtt
     2521 cggaaaaatc gcggagagct cgggccgctc tccacgtccc gctggaatag cggttatccg
     2581 gctgagattt atgacgtttc agccttagtt cggagacaac agtcctgaag cctctccaga
     2641 ccacgattta attttactca taacattaag atatagaact tcttttttga tggtaatcgg
     2701 gccgcaaaac tgggggcagt actcactagg ggatttccta taggccgcaa ttaaaggggg
     2761 atatttccgt gttttttcct gggagaggaa tcggttttat tctcgagcag ccgcaacgcg
     2821 cgtatcagct tcgggcgagt ttggcgcggc tcgatcacat cgtccacgaa acccctttcg
     2881 gcagctataa agggattggc gaagcggtcg cggaactcct ggatctttgc cttacgcgct
     2941 tcgtccgctc catcggctcc cgcgagttcc cggcggtaca cgatattcac cgcgccttcc
     3001 ggacccatca cggcgatttc cgccgtggga taagcgaaat tcatgtcggt gcggagatgc
     3061 ttcgacccca tgacgcaata ggcgccgccg tatgcctttc tcgtaatgac cgtaattttc
     3121 ggcaccgtgg cttcggcgta cgcatacagc agcttcgccc cgtggcgaat aatgccgccg
     3181 aattcctggt tcgtcccggg caggaatccg ggcacgtcct cgaaggtgat gatcgggata
     3241 ttgaaggcgt cacagaatcg cacaaaacga gcggctttca cggaagagtc gatgtccagg
     3301 cacccggcaa gatgggcagg ctggttcgcc acgatgcctg ccgggcggcc atccaggtgc
     3361 gcaaacccga tcactatatt ctttgcgaaa tgttcctgta cttcaaaaaa atcgccatca
     3421 tccaccacgc ggaggatgac gccccgcatg tcatagggaa cgtcgggctg ctccggaacc
     3481 acgctgtcga gctgcggatc ctcccggtcc gcggggtcgg aggtggcgcg caacggagca
     3541 tcgtccaggt tgttctgcgg gagaaaactg agcaggcggc gaaccatccg caggcaatgc
     3601 gcgtcgccgg aggccagaaa atgggccact ccgcttttct ggttatgggt catcccgccg
     3661 ccgagttcct ctttgctgac gtcttcgtgc gtgacggttt tgatgacatc gggtcccgtg
     3721 atgaacatat ggctggcgcc atccaccatg aagacgaaat cggtgagtgc cggggaatac
     3781 acggcgcctc cggcgcacgg tcccatgatg gcagagatct ggggaaccac tccgctcgcc
     3841 agcgtgtttc gcaggaagat gtcggcgtag ccggccagcg aaagcacccc ctcctgtatg
     3901 cgggcgccgc cggaatcatt cagcccgata attgggcagc cgttccgaag cgcaagatcc
     3961 atcaccttga caattttttg cgcgttggct tccgacaaag aaccgccgaa tacggtgaag
     4021 tcctgggcga atacacaaac cggtctgccg ttgacgtgac cgtaaccggt gacgaatcca
     4081 tcaccgtcga caacgtgttt ctccatgccg aaatcgcggc agcggtggcg cacgagttta
     4141 tcgagttctt cgaacgttcc ctcgtcgagc agcagttcaa cgcgttcgcg cgcggtgagt
     4201 ttcccttccc ggtgctgccg ctcgcggcgt tccggtccgc ctccagtctc cgcggccgcc
     4261 ttacgccgcc gcaactcttc cagcctgtcc atggccataa cagaacagta gcgtacgcaa
     4321 acgcgggaag ctacgcgggg ccggataccg cttgctgacg cgcgcggctc cgtggaaata
     4381 cgtaagcgaa tttctgaaaa tgtgtactta gcgaacgtaa tagccgctgc gcgcccggac
     4441 caccgcaccg gaatacctct ggcgcgctgc gggagtgagt tccacgcgaa tctgccggaa
     4501 tgttccggac tttgcttccg gagcgttgta ggccagggtg tatcgtgtgc ggatacgctc
     4561 catcatctcg gggaaggtct ggtcggcgcg gttcaccttc acggcttctc cgccggtctg
     4621 ctcggccagt ttgaacacat cggcaggagt gaaatccggg ttctggtaca cacccgggcg
     4681 tggcgcgccg ggacgctcgg ctttgcccac cacaatggcg ttcagcacgg tgtcttcccg
     4741 caacaactca cccgtgacct tggagtcggg cgtctggtaa ttcaagctca gattatcggt
     4801 gaggatcaaa acggcgcgcc ggtcctgccg gtttccgaac tcggactctt tgtatcgcgc
     4861 caggtaggat acgccatcga gaatggactc gttgatggcc gtgcccgcgc ccaggtcatg
     4921 ttcgttactg gaatcccgga tcttttccgc tacctgcacc agatcgcccg taaagggctg
     4981 cgtgacttcc gtctgcctgc tgaagagcat cacgcccacg cggtctccga cccgcagata
     5041 ccgcagagcc ttttcggccg tgcttgccat ctggtcgaga taccttctca tgctgccgct
     5101 cacatccagc agcaggagaa gagacagcgg atcgcggtcc tggccaaagt aggccaccgg
     5161 ttgcggtacg ttctcgtcga acacagcgaa atcgtcccgc gtgagacccg tgatcacgtt
     5221 tccatcgcgg tctgtcacct gtacgtctac ccttacctgt gtcacgccgg tgcggaagac
     5281 gggcgtttct tccgcaggcg ccggcgcctg atcctggccc tggcctgcca atctcatcgc
     5341 aggcagtacg atgagaatac aaagaatgaa aaacctgacc atcggcacgg cagggcacat
     5401 cgaccacggc aaaacagcgc tggtgcgcgc tttgaccggt gtcgataccg accggctggc
     5461 ggaagagaag cgccgtggca tctccatcga tctgggcttc gcacacatgg atttatcgtc
     5521 cgggcttcgc ataggtttcg ttgatgttcc gggccacgag cgatttgtca aaaacatgct
     5581 ggcaggcgtg gccggaatcg atatggttct tttcgtgatc gccgccgatg aatccattaa
     5641 accgcagacg cgcgaacact tcgatatctg ccgtctgttg gacgtcggga agggcattct
     5701 ggttttgaca aagtcggatc tggtcgatcc ggacattctc gaactggtaa agctggaagc
     5761 cgaagatttt gtgcgaggct cgtttctgga gggcgctccc attgtggcgg tcagctcgac
     5821 aacgggagag ggcctggagc agttgcgcgc ggaaatcgga caaatggccg cctctattcc
     5881 gccacgcaat gcgggcgggt tgttccggct ccccatcgac cgctctttct ccatgaaggg
     5941 cttcggagct gtggtgacgg gcacgctgat cgccggttcg gtcaggaccg aacaggaggt
     6001 ggaagtgcag ccttccgggc agcggtttcg cgtccgggga atccaggtgc acggtgaagc
     6061 tgtagagagc gcggcagcgg ggcagcgcac tgctctgaac cttgccggcg cggaccatcg
     6121 ccacctcgcg cgagggatga tgcttaccgt gccgggcctg cttcgtccaa ccacccggct
     6181 cgattgcctg ctgtatctgc tgccgttggc gaaacctctg aaacacggcg ctccggttca
     6241 ctttcatgcg ggtaccgcgg aggtggaggg cgagattcga atgctggatc gcctttcggc
     6301 gcttgctccc ggcgagagcg ctttcttaag gattctgctg cgcgaccctg tcctgctgct
     6361 gccgggagac cgctacatcg ttcgtatgtt ctctccggtc gtgaccatcg gaggcggacg
     6421 cgttatcgac atcgatgtgc ctcgccggat ttcccgcgcc ggggcgttcc ggcgtatgca
     6481 atccctctct aacgcggacg atgccacgcg cgtgtcgacg ctggtacggg aatcggcctg
     6541 cggcttgtcc ctggccgagc ttgtggcccg gacgggcctg ccgcttgcct ccctggagac
     6601 tctcgcccgc cgcgacgagt ttgtcgcccc gggcggttgg ctggtggacc gcgactggtt
     6661 caatgccgcc tccgccggct tgagccggat cctcgaggac tttcatcgtg ccaacccgct
     6721 gcttcccggc attcccaagc aggacctgcg aggccgccaa ttgcccgatt gtccgccacc
     6781 gcttttcgat gagctggttc gtttcaacaa ggggctggta gccgacggcg agttcgtgcg
     6841 gtccgccacc catcgaatta cgctgcagga gaacgaggca gaagcactgg agagaatgga
     6901 gcgccttttt cgggatgcgg gtcttactgt cccggccacg aaggatgtgc tggcgcggtc
     6961 cggccttgac gctgcgcgct cccgccgtct gcttcagatg cttttgcgcg gcgggaaact
     7021 cgtgagagtg ggagaagacc ttgtctacca tcagactgct ctcgaaggac tgaaaagcct
     7081 tctggcaggt catgtgggcg agcgtttcgc cgttccggag ttcaaggact ggaccggcat
     7141 ttcgcgcaag tacgcgattc cgcttctcga atacctggac cgcgaggggg taacacgcag
     7201 ggaaggcaat gcccgcgtgg tactatccgc gctcccggcg cgcaaggctt gacacagggc
     7261 gaataaaaga cgaatatacc gggaagaggg aggggatttg ccgtgctttt tcccgaattc
     7321 gcacgcccca cgctcatcgg aatcgccaga caggcagccg ggagcgaatt gctggaagcc
     7381 aaggcccggg tccagtattt cgaactgcct acgcgccgtt ttctgagccc cgtacgcagc
     7441 cggcgtgtgc cgttctgctg ggccatcaac ccctaccggg gctgcgaatt cggttgccgc
     7501 tactgttacg cacgctacac gcacgagttc atggaacttc gcgagatctc tcaattcgag
     7561 gagatgattt atgcgaagca gttcgataaa gccgcgttcc gccaggaact ccgctccacg
     7621 gcgcggcagg agtccatcgc catcggcacg gggacggatc cctatcagcc cgccgagcgg
     7681 cggttcggcg tcacaagaca gatcctcgaa gtccttgccg gcgaagaggg ccgccgcatt
     7741 tcactggtta ccaaatccga tctggtcgcg cgggacgcgt tgctgtttca aacaattgcc
     7801 caatcaaatg ttttgcatgt caatatcacc attacgacgc tggatgagcg gctggcgcgt
     7861 ttgctcgaga cctgggcacc gcggccggcg cttcgggtgg ctgccgtgcg gaccctggtc
     7921 gaggcgggta tacgagtggg agtgctgcca aacccgattg tgccgctgat tacagacagt
     7981 gaagaaaatc tggactcggt ggcggccgcc gccgcccagg ccggcgctga atcgttcggt
     8041 ggtggagtgc tgttcctgaa accctgctca cgccgcgtgt tcttcccttt tctggcggag
     8101 cattttccgc acctgctgcg gcggtaccgc gagcggtatg aagaatccgc ttttttacac
     8161 gggtcctatc cggagtccat tcgggagcgc gtgcggcgtg tgaggcagcg gcatggcttg
     8221 gtgggcggcg gcgcgaacta tgaacccgaa ctgtgggaag gcgaacctca actggcgctg
     8281 ttcgatgatt ctgctttgta ggaataagac tgcaaaacat tgttgtcaat ttcacccaag
     8341 catgtccgat aagtagatag gaagtctgtg ggccggcggt gtgttcgcgt acaaacggta
     8401 cgggcaactt cgtgtgccgg ccgatacgtc agcgtcgcaa gatgtccgtt cggaccgacc
     8461 atacttcctc tgaacagcga tggcacaccc aggagggatc acacaaggtc ctcacaaccg
     8521 cgcacgcatc gtcgccgaca tgctgcaacg gtcatttgcc ggaccaatcg caaacgtgtt
     8581 tgccatcgtg gtatttctgt tcgggacgga cctgcttcag caacacccgt ttgtgacatc
     8641 gttgtttctc ggcatcctca ctcttcggct gcttgggcgc gcagtgttca catgcctccg
     8701 ccggaaaagg atgcccgcgc gcgagccacc gctctggctg atcggcttct ctgcctatgc
     8761 gctctcggtg ccgaccggat gctatgcggc attcgttctg cactcgtacg ggtacgaaga
     8821 ctggaatacc gtgctgatgt atgtcttcgc catggtctgt gcgatatcgg gcaccacggc
     8881 tctggctccc gatctgcggc ttgggctggg ttatcaggct tcgttattgt cgcctgtggt
     8941 catcgcttca ctggcggcag acggccggcg ctgttgggtg ggagcgatcg ccgccatcct
     9001 ttttgcggcg tacgccgtga tgcaggcggt ccggcaaaat acggagtacc ggaacgccca
     9061 ggcggccagc gacgctcttc gatgccgtgc ggaagaactg gaggtggcgc aagccgccgc
     9121 ggccgcggcg agcgaagcga agagccgttt ccttgcgaac atgagccacg agatccgtac
     9181 accgatgaac ggagtcctcg gcatgctcga actggtgctc cggagcgatc tgtcgtcctg
     9241 ccagcggcag gacctcgact gcgcgcgcga ctccgcacag tcgctgctgg tgttgctcaa
     9301 ccagatactg gatcacgcaa aggccgaggc aggccgcctg gaactggagc aaacggattt
     9361 ctctgttcgt gaagttgtat gctccgcggc ggttccgttc cgtttccagg cggccgaaaa
     9421 ggaaatcgcc ctgagttgta ccgtcgagcc ctccgtgccg caatgtctga ggggcgatcc
     9481 gactcgtctg cgtcaggtca tcgtcaatct cgttgcgaac gcgctgaagt tcacgccgtc
     9541 gggctcgatc aaagtcgctg tgcgtgtgga gtccgtcacc gggcccgatg tgcttctcgg
     9601 cttccgcgtg gccgacagtg gaccgggaat tccaaccgac aaacaggaga cgatcttcga
     9661 tgcgttttca caagctgaca gctccatcac acgccgtttt ggcgggacgg ggctcggttt
     9721 ggcgatctgt cgcgatctgg tggaactgat gggaggccag attcgggtcg agagccaacc
     9781 cggacgtggg agcgtattca gctttaccgc catgttcacg atgtcgtcca gtgaaaagcg
     9841 ggagaccgaa cccgcccggg ctgagccgcg tgtgtgcaat ccgctcagag ttctgatcgc
     9901 cgaggacaat gttgtgaatc agaaaatcct catgcgcatt ctgcaaaccg gcggacactc
     9961 cgtagtgctt gcggaagacg gtcttcaggc cgttgcacgg ttctcggagg attggtttga
    10021 tgttgtcctg atggacgttc agatgccgcg catggacggc ttgcaggcaa cccggcacat
    10081 tcgagccgcg gcgggcagag acgtccccat cattggtgta acagcaggtg cgactcctgc
    10141 cgaggttgcc gcctgtttcg aagcgggaat ggattcctgc atcacgaaac caattaacat
    10201 ctccaagctt caggaactgc ttgcgcgagt gcgggccggg aacctgccgg aaggccagcc
    10261 tagccgttgc ggctgattga agccgcaggc acaaaatttc cccttggcta tctacaatac
    10321 ctggcactat tgtagatagt cgagaggagt aggggacttt gggcaaacct tccgatttga
    10381 tccagggcac gctggacctg ctgattctga agacgctgtc gctggagccg atgcacggct
    10441 ggggaattgc gcaacgtgta gaacaggctt cgcgagatgc gttgcagatc cagcaaggct
    10501 cgctctatcc ggcgctgcac cggctggagc agcaaggctg gatccgctcc gagtggcggg
    10561 agacggagaa tcgcaggcgc gccaaatatt acctgttgac cccggccggc aaacggcagt
    10621 acgcaaaaga gctggcgaac tggcagcgcc tgtcgcaggc gatcgggctg gtgctggaga
    10681 tggcgtgatg cggtggcttg atcgcttacg ccttcgtttc cgctctctgc tccggcgtgg
    10741 acgcgtggag cgcgagctcg accaggagct gtcgttccat atcgaagagc tgacgcgccg
    10801 gcagatcgaa cggggcatca cggccgaaga ggcgcgcact gcggcgttgc ggcaattcgg
    10861 cggcgtcgct caattccagg aagcctgccg cgatcagcgg cgtacgcgat ggatcgaaga
    10921 cttctttcac gatctccgat atgccgtgcg gcttttcgca aagagccccg gatttacggt
    10981 agttgccctg ttctccctcg cgctgggcat cggcgccaac accgccgtat tcagcctgtt
    11041 ggaaacgatc gtacttcgcg agctgcccgt gcgggaaccg ggagcgctgg tgcagatcgt
    11101 gggtagggat ggaaggggag cgcaaggctc tttctcctac ccgtcgtacc tatggttgcg
    11161 ggagaacgcg ccgatgttct cgcacgtgtt tacctggcga ttcaccaacg tctgggccgg
    11221 ccagggcgac tcgatggaat ggatcgctgt cgacaaggta tccgaagact acttcgccgg
    11281 actgggcgtc tccgccaccc tgggccggac actgggcgac gaggacgggc agccggcggt
    11341 cgcggtcctg agccacaaat ggtggaaggt gcgattccag gccgatccgg atgttgtcgg
    11401 gcgagagctg cgtctcggag gtatccaatt cacaattgcc ggcgtgatgc ccgcagggtt
    11461 ctccggcgcc gaggtcggca aggccgcgga tgtatatata ccgttatccg ccgaacggct
    11521 gctcgcaccc ggcagcgaaa tactgttacg gcgcaacgcg tcatggcttc ctttgatggg
    11581 ccggcttcgc gggggtttgc cgcggcaaca ggcgacggcg gaattcgagc cggtatgg
//
LOCUS       DASTMK010000386        14013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_146014, whole genome shotgun sequence.
ACCESSION   DASTMK010000386 DASTMK010000000
VERSION     DASTMK010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146014"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..997)
                     /locus_tag="VFQ79_16745"
     CDS             complement(209..997)
                     /locus_tag="VFQ79_16745"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0141373.1"
                     /translation="MKRIIAFAYGIACYGVFFATLLYAIGFLGNFGVPKSIDSGREGS
                     VFTALVIDALLLALFALQHSIMARPWFKRVWTRIVPVPVERSTYVLFSSLALLLMFWQ
                     WRPVGGVIWNIDGGTARMIVFGLYAAGLLIVLVSTFLINHFDLFGLRQVYLYLAGRKY
                     TQLEFRTPFFYRYVRHPLYVGWLLAFWAAPVMTVAHLFFAVMTTAYILVAIQFEERDL
                     IDMHGEKYRRYRKQVPMIIPSLQTGVNSEQEPVSTPTPIAVSNQ"
     gene            1123..2121
                     /locus_tag="VFQ79_16750"
     CDS             1123..2121
                     /locus_tag="VFQ79_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU0141374.1"
                     /translation="MLIAPLDALSEVLKFVTLKGALFYNAEFSAPWSVRSPASAAIAT
                     HLTAEAAHVIIYHLVTEGGAWVQLETGPRIDLRAGDIVMFPHGHPHLMGSGAPAVPVD
                     NEKELQRILSQGLEVARMGGGGQITKLVCGYMVCEPRINDLLLSGLPSLLTIHIRNDP
                     SGVWLEDSIRFFMVHAASSAPGAEAVLAKLSEVLFVETLRRYIALLPTTETGWLAGAR
                     DPEVGKALALLHREPARPWTIADLAASAGVSRAVLAERFRHYLGEPPITYLRRWRLNL
                     GARLLTTTSHSVAQVAALSGYDSEAAFNRAFKGSFGVPPARFRRNSRSKRTAGAST"
     gene            complement(2092..3255)
                     /locus_tag="VFQ79_16755"
     CDS             complement(2092..3255)
                     /locus_tag="VFQ79_16755"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0141375.1"
                     /translation="MIRRFDAVIVGAGAAGLMCAIEAGKRGRRVAVLEHAEGVGKKIL
                     ISGGGRCNFTNIYSSPDNFLSANPHFAKSALARYTPADFVTLVEAYGIPYHEKTLGQL
                     FCDRTARDIIHMLEEECRRAGVTIFLKTSVREVRRTTEFVLDTGTEEFRASVLVVATG
                     GLSIPKMGATGFGYGVARQFGLKIRECRPALVPFTFGREDRGRYSSLAGVSCEVVASI
                     GKQRFREKMLFTHRGLSGPAILQISSYWTQGQPIMIDLAPGGDFTPALLEKSHRDTGA
                     AKSVLRGMVPARLAELWMDLNAPARWTNPAILELERRLHHWQVIPAGTEGYEKAEVTA
                     GGVDTNELSAKTMECRKQTGLYFIGEVVDVTGHLGGFNFQWAWASGACAGRAL"
     gene            complement(3277..4467)
                     /locus_tag="VFQ79_16760"
     CDS             complement(3277..4467)
                     /locus_tag="VFQ79_16760"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0141376.1"
                     /translation="MIFPLLAVNAADTRPVPLFDGKSLKGWQQCNGSATYRVEDRAIV
                     GTTAEGSPNSFLCTDREYGDFVLEFETRTDPALNSGVQIRSHRYPAETVVRTFDGKQT
                     REHKQPAGRLYGYQVEISNEESGTSGGIYDEARRGWLDNTSSKPACKAAFKDNQWNRY
                     RIEARANVIRTWVNGAECANLVDSADLSGYIALQVHSYKGDKPVQVRWRNIRLTDLGK
                     HVWRPLWDGKTLKGWLNTGGGKWTIEDGAIHGQSVPGDERIGFLMTEESFKDVTARVR
                     FKIPKGNSGFFVRIDPETRAGYEIEIDAQKRTGGFWEVRGRNWVTGPEDNANVRTDDW
                     NEITGFFHGHRIVFHLNGVKTIDFPNDTQGRLEGRIGIQAHGSKMPTDVWFKDIAVLE
                     PERK"
     gene            4544..6961
                     /locus_tag="VFQ79_16765"
     CDS             4544..6961
                     /locus_tag="VFQ79_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141377.1"
                     /translation="MQRRHAGNKLLFLCAAALFPPAIVAQTSLEPARAVVLGIHRYTD
                     VLGIDPLEYAGKDAALFESLLRSGRLGTSFGPSGIVPLTNSGQIATRAGLRDTIGSAI
                     PGETLFIFISGRGLAYPGWDEGYIACSDTQAEKPQSTGVSVKELKSWIDGSPATRVWL
                     FADVCRQSSRRLDNRINYRFADEFKKIASKPFGAVLATSQKQQSEESGTLRSSLSDGH
                     GVFSYFLVNALYGRVSAGTKDADTDGDGRVSTKELLAYLLDRQRPPFTKTPQVPEAFG
                     SLTKANAAVVVLGARRGAIRPQAEPVLLASLGAASLKFFPMQAGAAVPQATEAGCPSL
                     SNEEIANPVRAAEAFLGARPQTGSGEWNRRAYCLALAWESQGQRVVAAYGIGDQFPDD
                     PAKLKESDFLAAARAFEAAYKLRPSDESLRVRQLFCDGRARAFSNATEARRLLGAALA
                     VPTSGAEPAAEIHNAFGLTLFESGEHKSAIPHLEEAIRRAPTWAYPRHNLALAYTELG
                     NYIAAEREYREAIRRTPYYPYLYYNLGLLLHRTGRNRAARQEYQLALTVSDEGIHKWR
                     DAAARWGREGHTADADLAAARAEAFRRNKGEIYNALGALLEAEDALVQAAEEYQHALD
                     MNPELFVAAYNRASVVDRAAVRQTPGTVAAEALKLLRSVRDRNERFYPSRLLLGSLLL
                     RQREFDEAEAEFRAVLQSGSAPVDAHAGLARALLGRKDFDGARIHAEQAIKQQTAAIR
                     IENPSSKRTPLAQAALYQVLADTFREMQDRSRACEFYRTAQQALKWNREGVDLRELSR
                     KANECSR"
     gene            6949..10200
                     /locus_tag="VFQ79_16770"
     CDS             6949..10200
                     /locus_tag="VFQ79_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141378.1"
                     /translation="MLAVKRLAAIALLLPCLTAPVLPQSTQALISGRVYNRDIGFGVA
                     GAVVTATHNASGLRQRAVTGAFGYYWLPSLSAGTYSITVTAQGYQPRAAFDVTLFTAG
                     FLTLDFPMRALPAAAWHGEYVPGYLPSTDAIVHIYAADVETTAAEPLSALKSRQGVAE
                     ASQSYVIAPEEIERLPLAGRDVYATVALQPGVTSQPASARGLGLSANGQRPTSSNFLL
                     DGVQINDPLVTGPLTLMAPEAVQEYRVSVGTSSAEYGGAAGLIANAVSKAGTNDWHAL
                     AYWNVRHDALNAQDFEQKRLGYNKPPLREMQEGFVAGGPLSPGRVFGSLAFDAVRFRS
                     RRDPVPYWLPSASFLASLPDGSAAGRLLRQYPAVAVPSAGAGMAQIELAPTASINRYL
                     AAPRADAILDGGAHHIMSRVAIARLHQPDLLYSPYEQFNAPYNQNLLNVAAAWTWSAS
                     PRLTNELRAGYGELEADVDRPHPEVPVLLAPRVTLPGTQSFYAFQTKASSVEVSNTTA
                     LAWGRHLFKWGGFYLNRRIDNFTSMGRDGIYYFDSLDDFAAGNSSALLGSVARGLQGL
                     SEPGYRRRYGYGNAALFAQDSFRPHPRLTLSFGVRYERFGTPKSLRDATDFLVAWGQG
                     NDVFERLQGAQLARRGGEHDMFEGQPHHWAVRFGIAHEILPRRGTVLRAGYGIFYDRI
                     FDNLWVSAATNAVAFGFWHFGHPVDVLLPLAFNKAMAADFAPTDLLDYVAFAPQFSAG
                     RAHNFFTSVQQPLTRSVWVEATGAGSLGRGLVTTDRINRYGSVPRTPDNLEQRIAPGL
                     PQLAYRANQGDSDYFAGTALVRFRAANITGQVAYTLSHMIDVQSDPVADDYLDLNWFR
                     RAQTAAPRPVPSFTRQFRPGLDRANSDFDQRHNLVFFAALDVPRVERLKWLARDWRIS
                     LLGAVRSGTPYTVYVSAPGTGELIVNNRPDLIAPAAVRDGSSVNGGRRLLNPSAFQFP
                     ADGQVGSTGRNAFVGPGQFSFDFSVARRLPFRFGDRSCAVTFRADLYNALNHANPGNP
                     LNTTLPHPQFQSPNDSEFAVARYGRRGRAAGVPLLVPLSETARQIQLMLRFEF"
     gene            10282..10755
                     /locus_tag="VFQ79_16775"
     CDS             10282..10755
                     /locus_tag="VFQ79_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141379.1"
                     /translation="MRNPIRTFFAAALLLASATQLPAGGLFVMLGNPEASPEARKHNA
                     VLTLTLAGCHEPEKVPVEGTAITVVDGKRQTVPIQLIALSKPGMYAVTRQWPAKGRWV
                     LQFVANDQGRAASTLVAATPGKIERHSAKMAMRLPSEEDVAALLSGEDKPEVARK"
     gene            10761..11327
                     /locus_tag="VFQ79_16780"
     CDS             10761..11327
                     /locus_tag="VFQ79_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0141380.1"
                     /translation="MTARLMPLTFALRTPAALAFDHAARVRAAAGGDRAAFDELYIRY
                     ASMVHGILLARVPVFVAEDLVQDVFLIALRKLSGLRAPEAFPGWLAAIARNRAVEYHR
                     SARHESDVHAEAARRQVQAPASNALDVLRVIRKLPEAYRETLILRLVEGMTGPEIAEQ
                     TGLTPDSVRVNLFRGMKMLREELGGGTK"
     gene            11324..12289
                     /locus_tag="VFQ79_16785"
     CDS             11324..12289
                     /locus_tag="VFQ79_16785"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEU0141381.1"
                     /translation="MNDYLWDGSGRPEPEIEALERTLAPLRFPVAKRPLQLPHSSRQA
                     IPYAAIAASIVLGLVVWQLFPTHPPQLTAWTVSEDGDAAAVRKVLVGERLKTGESSRL
                     DLESDGFGRIEIRPDSEMRFLESAAGRQRMELKHGSIHAFIWAPPQQFIVDTPSATAV
                     DLGCQYELSVDKSGNGFLTVQTGWVAFQLGDIESFIPAGAACRTTPAGGPGSPFFEDA
                     SIAFRNALEGFERFGDQKMLDRVLVESRPRDGLTLWHLLARVRETERAAVFDRFAQLV
                     PLPAGVARDRAIARDRATLNLCWNALELDSTDWWRTWKQQWRGRR"
     gene            complement(12328..12591)
                     /locus_tag="VFQ79_16790"
     CDS             complement(12328..12591)
                     /locus_tag="VFQ79_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0141382.1"
                     /translation="MELGDNAEVIRELVKQGADVNARTRYEVQTPLHIAAAMGKMKAI
                     EALVALGADLTIKDSEGQTPLDAAERAGLTDVAAFLRRAAAAK"
     gene            complement(12761..13981)
                     /locus_tag="VFQ79_16795"
     CDS             complement(12761..13981)
                     /locus_tag="VFQ79_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEU0141383.1"
                     /translation="MARTVASLPAGSRITDYISLGVIAKYFPREKVDAVLEQTGRASL
                     RERDLPAHVVVYYVIALALYMRSSYREVLRCLLEGVQWLLNPSASVKVAGKSGISQAR
                     SRLGVEPMQALYEAMVAPIAGKRTKGAWYRQWHLTSLDGSTLDVADTKENEEAFGRPG
                     ASRGSAAFPKIRFAALLENGTHVLWAARVDKYATDELTLAEEVVPALQKGMLCLADRF
                     FPSHKLWQKAAATGADLLWRTRQNARLEIDERLPDGSYLSRIYPSTSDRRNERKAMVV
                     RVIDYRLDKVPDAEPVYRLITTILDHKLAPAKELAALYHERWEIETALDELKTHLRGA
                     QIVLRSKTPELVKQEFYGLLMAHFAIRGLMHEAALKADEDPDRLSFLHTVRVVQRRMA
                     RFAAIPPSAQKSTS"
BASE COUNT         2715 a         4378 c         4122 g         2798 t
ORIGIN      
        1 tgaggacaaa gatccccatt gcaaaaaaca aaaatctcct ctgcattgtg atttcctccc
       61 caagttgtgt ggctaacacc cagtgagtcg atcgtatacc catgcagaga agtaagtcaa
      121 aggaaaacgg actgaataca caactcgccg ccaccggctc cgtgaggagt gggcgagatc
      181 gtgtccccac ccctcgtatg tggagtgtct attggttcga cactgcgatt ggcgttggtg
      241 ttgaaacggg ctcctgttct gaattaacac ctgtttggag agatgggatg atcatcggca
      301 cctgtttccg gtaccgccgg tacttttctc cgtgcatatc tatcaggtcg cgctcttcga
      361 actggatcgc taccaggatg taggcggttg tcattacggc gaagaacaga tgagctaccg
      421 tcatgaccgg cgcggcccag aacgcaagca gccaaccaac atagagcggg tgccggacat
      481 agcggtagaa gaacggtgtc cggaactcca gttgagtgta cttgcgccca gccagataca
      541 agtacacctg ccgcaatcca aacagatcga agtgattaat cagaaacgtg ctgaccagca
      601 cgatgagcag gccggctgcg tacagaccga agacgatcat tcgggccgtt ccgccgtcaa
      661 tgttccagat cactcctccc accggccgcc attgccagaa catcaatagc agcgccaggc
      721 tcgaaaacaa cacatatgtg ctccgctcta ccggcaccgg cacgatgcgc gtccacaccc
      781 gcttgaacca tggccgcgcc ataatgctgt gctgcaatgc aaacagcgcc aggagaagag
      841 catcgatcac cagcgcagtg aacaccgaac cttcgcggcc ggaatcgatc gattttggca
      901 cgccgaaatt tcccagaaaa ccaatcgcat ataacaacgt ggcaaagaac acgccgtagc
      961 acgcgatacc gtaagcaaac gcaatgatcc gcttcatttg aatgtcctcc tgttggaatc
     1021 aaatctgata ccagccgctg cccttcatag agagaagcgt tgaaagcggc accttgaatt
     1081 tgcccgggcg tctgtttgtt ttgctccggc ggcggtaaga tattgctaat cgcacccttg
     1141 gatgccctgt ctgaagtact gaaattcgtt acgctcaaag gcgcgttatt ttataatgcg
     1201 gagttttccg ctccatggag cgtccggtca ccggcatccg ccgcaatcgc gacgcatctg
     1261 accgcggaag ccgcgcacgt catcatctac catctcgtga cagagggcgg cgcctgggtc
     1321 caactcgaaa caggcccgag gatcgatctc cgcgccggtg acattgtgat gttcccgcac
     1381 ggccatccgc acctgatggg cagtggagct ccggcggtgc ccgtcgacaa cgaaaaggag
     1441 ctgcaacgaa ttctgtcgca agggctcgaa gtagctcgaa tgggcggtgg cggccagatc
     1501 acgaaactgg tgtgcggata catggtttgt gagccccgga tcaatgatct tttgttaagc
     1561 ggacttcctt cgctgctcac catccatatc cgaaacgacc cgtccggtgt gtggctcgaa
     1621 gattccatcc ggttcttcat ggtacatgcc gcttcgtccg cgcctggggc ggaagctgtg
     1681 cttgcaaaac tttccgaagt cttattcgtt gaaacactgc gccgctatat cgcgctgtta
     1741 ccaactaccg agacaggctg gctggcaggt gcgcgggatc cggaggtggg aaaagcgctc
     1801 gccctgttgc accgtgaacc cgcgcgtccc tggacgatcg cggacctggc ggcgtcggcc
     1861 ggagtgtctc gcgcggtatt ggcagaacgt ttccgtcact acctgggtga gccgccgata
     1921 acctacctga gacggtggcg cctgaatctg ggtgcacgct tactcacgac gaccagccac
     1981 agtgttgccc aagtcgcagc cctctccggc tacgactctg aagctgcttt caaccgtgcc
     2041 ttcaagggca gtttcggcgt accgccggcc cgtttccgga ggaactcaag atcaaagcgc
     2101 acggccggcg caagcacctg atgcccaggc ccactggaaa ttaaaaccgc cgagatgccc
     2161 ggtgacgtcc acgacttcgc cgataaaata gagccccgtt tgtttgcggc actccatggt
     2221 cttggctgat aactcattcg tgtcgacacc gccggcggtc acctccgcct tttcgtagcc
     2281 ttcggttccc gcggggatca cctgccaatg gtgcagcctc cgctcaagtt ccaggatggc
     2341 agggttggtc cagcgtgccg gtgcgttcag atccatccaa agctccgcca gccgggccgg
     2401 aaccatgccg cgcaataccg atttcgcggc gcctgtatcc cggtggctct tttcgagaag
     2461 cgctggagtg aaatcgccgc cgggggcaag gtcgatcatg ataggttgtc cctgggtcca
     2521 gtaagaggag atctggagaa tcgccggacc gctcaatccg cggtgtgtga acaacatttt
     2581 ttcacggaaa cgttgtttgc cgatggaagc aaccacttcg cacgacaccc cggcaagact
     2641 actatagcgt ccgcgatctt cccggccgaa tgtgaaagga accagcgcag gccggcattc
     2701 gcgaatcttc agaccgaact ggcgcgcgac gccatagccg aagccggtag ctcccatctt
     2761 cggaatggag agcccgccag tggcaaccac cagcacggac gcacggaatt cctccgtgcc
     2821 tgtgtcgagg acaaactcgg ttgtgcgccg cacttcgcga acagacgttt tgagaaagat
     2881 cgtgacgccc gcgcgccggc actcttcctc cagcatgtgg atgatgtcgc gcgccgttcg
     2941 gtcacagaac aactggccga gcgttttctc gtgataggga atgccatacg cttccaccag
     3001 ggtcacgaaa tcggcgggcg tgtagcgtgc aagggcggat tttgcgaaat gcggattcgc
     3061 cgaaaggaaa ttgtccggac tggaatagat gttggtgaag ttgcagcgtc cgccgccgga
     3121 gataaggatc ttcttcccca ccccttcggc gtgctccagc acggccacgc gccggccacg
     3181 tttgccggct tcgatcgcac acatcagacc cgcggcaccc gcgccgacga tgactgcgtc
     3241 gaaccggcga atcacgttat ggagcagctt actgttctac ttccgttcgg gttcgaggac
     3301 agcaatgtcc ttgaaccaaa catcggtggg cattttgctg ccgtgcgcct gaattccgat
     3361 cctgccttcc aggcggccct gcgtgtcgtt gggaaagtcg atggtcttca cgccattgag
     3421 gtgaaaaacg atccgatgtc cgtggaaaaa gccggtgatc tcgttccagt cgtccgtgcg
     3481 cacgttcgca ttgtcttcgg gtcccgtgac ccagttgcgg ccgcgcacct cccagaatcc
     3541 gccggtccgt ttttgagcgt cgatctcaat ctcgtacccg gcgcgggttt cgggatcgat
     3601 gcgcacgaag aaaccgctgt tacctttcgg aatcttgaat cgcacgcgag cggtgacgtc
     3661 tttgaaggat tcctccgtca tgagaaagcc gatgcgctcg tcgcccggta cgctctggcc
     3721 gtgtatggct ccgtcttcga tcgtccactt gccgcccccg gtattgagcc acccctttaa
     3781 tgtcttaccg tcccaaagag gacgccagac atgcttgcct aaatcggtga ggcgaatgtt
     3841 gcgccagcgg acctgcaccg gtttgtcgcc tttataagag tggacctgaa gcgcgatgta
     3901 gccgctcaga tcggcagaat ccaccagatt ggcgcattcc gcgccgttta cccaggtgcg
     3961 gatgacattg gcccttgctt caattcggta gcggttccac tggttatcct taaacgcggc
     4021 cttgcaggcg ggcttggacg acgtgttatc gagccagccc cggcgcgctt catcgtagat
     4081 gccgccggaa gtgccgctct cttcgttcga aatctccacc tggtagccat acaaacgtcc
     4141 ggcgggttgc ttgtgctccc gcgtctgttt gccatcgaaa gtccggacca cggtttcggc
     4201 gggatagcga tggctgcgaa tctgcacccc cgaattcagc gcaggatccg tcctcgtctc
     4261 aaactcgagg acgaaatcgc catactccct gtccgtacaa agaaagctgt tggggctgcc
     4321 ctcggcggta gtgccgacaa tggccctgtc ttcgacccgg taggttgccg agccattgca
     4381 ctgctgccag cctttgagcg atttgccgtc aaacagggga accggcctgg tatcggcggc
     4441 gttgacggcc agtagcggga aaatcataca aattatgagg cgatgcacac ggatattgta
     4501 acctcggatt ccgctgtgcg ttggcgtata ctggaccaca cacatgcaga ggcggcacgc
     4561 gggaaacaaa cttttgttcc tttgtgccgc agctctcttt ccgccggcca tcgtcgctca
     4621 aacttccctt gagcccgccc gggcggttgt actcggcatt catcgctaca ccgatgtgct
     4681 tggtatcgac ccgttggaat acgccggcaa agatgcggcg ctgtttgaga gcctgctccg
     4741 gtcggggcgc cttggaacgt cgttcggccc gtccggcatc gtgccgctga cgaactccgg
     4801 ccagatcgct acgcgcgcgg gcctgcgcga cacgatcggc tccgccattc cgggagagac
     4861 cctcttcatc tttatttccg ggcgcgggct cgcctatccg ggatgggatg aaggctatat
     4921 cgcctgctcc gacacgcagg cggaaaagcc gcagagcacc ggcgtgtccg tcaaggagtt
     4981 gaagagctgg atcgatggct ccccggcaac gcgcgtatgg ctgtttgcgg acgtgtgccg
     5041 gcagtcctcc agacgcctgg ataaccgtat caactatcgt ttcgcggatg agttcaagaa
     5101 gatcgcttcg aagcctttcg gagccgtgct ggctacctcc cagaaacagc agtccgaaga
     5161 aagcggaacc ctccgttcaa gcctgagcga tggtcatggt gttttcagct actttcttgt
     5221 gaacgcgctt tacggccgtg tttccgcggg aaccaaagac gccgacaccg acggggacgg
     5281 gcgcgtcagc acaaaggaat tgctggccta cctgctggat cgccagcgcc cgccgttcac
     5341 caaaactcca caggttccgg aagcattcgg gtcgctcaca aaagcaaacg ccgccgttgt
     5401 cgtgctggga gccaggcgcg gagcgatacg ccctcaagcg gaaccagtcc tgttggcatc
     5461 actcggcgcg gcgtcactca agttctttcc gatgcaggcc ggcgcggcgg ttccacaggc
     5521 caccgaagca gggtgcccgt cactgtcgaa tgaggaaata gccaaccccg ttcgtgcagc
     5581 ggaagcattt cttggagcgc ggcctcaaac cggaagcggc gaatggaacc gcagggccta
     5641 ctgtctcgcg ctggcgtggg aaagccaggg gcaacgcgtg gtggctgcat acggaatcgg
     5701 agaccagttc cccgatgatc ccgccaaact caaggagagc gacttcctcg ccgcggcgcg
     5761 cgcgttcgaa gccgcttaca agctgcgccc ttccgacgag tcgctccgtg tccggcaact
     5821 cttttgtgac gggcgcgccc gcgcattttc caatgcaacg gaagcccgcc ggcttctcgg
     5881 ggcggcgctg gcagtaccca ccagtggagc ggagccggcc gctgaaatcc ataacgcctt
     5941 cgggctcacg ctttttgaaa gtggagaaca caagagcgcg attccgcatc tggaagaggc
     6001 aatccggcgc gctccaacgt gggcttatcc acggcacaat ctcgcactgg catacacgga
     6061 gctgggaaac tacattgccg ccgagcgcga gtatcgcgag gcgattcgcc ggacgcctta
     6121 ttacccatac ctctattaca atctcggact gcttctgcat cgcacagggc ggaaccgggc
     6181 cgcgcgccag gagtaccaac tggccctgac agtctccgac gagggcattc acaagtggcg
     6241 ggatgctgcc gcgcgttggg gacgggaggg ccacacagcg gacgcggatc tcgctgcggc
     6301 gcgcgccgaa gccttccggc gcaacaaggg cgagatctac aatgcgctcg gagcgctcct
     6361 cgaagcggag gacgcgctcg tccaggcggc ggaagagtat cagcacgctc tcgacatgaa
     6421 cccggagttg tttgtagcgg cctacaacag ggcctccgtg gtcgatcgcg cggctgtccg
     6481 tcagacgccc ggcacggttg ccgcggaagc gctgaagctg cttcggtcgg tacgggaccg
     6541 caacgaacgg ttctatccat cccggctgct tctcggctcg ttgttgctcc gccagcgcga
     6601 attcgacgag gcggaagcgg agttccgggc tgttctccaa agcggctcag cgcccgtgga
     6661 cgcacatgcg ggtctcgcgc gcgcgttgct cggacggaaa gatttcgatg gtgcacgaat
     6721 ccacgctgaa caggcaatca agcagcaaac cgcggcgatc cggatcgaga acccttcctc
     6781 caaacgcacg cccctggcgc aagcggccct ctaccaggta ctggccgaca cgttccggga
     6841 aatgcaggat cgcagccgcg cctgcgagtt ttaccggacg gcccagcagg ccctcaagtg
     6901 gaatcgtgag ggagtcgatc ttcgcgaact ctcgaggaaa gcgaacgaat gctcgcggta
     6961 aagcggcttg cggcaatcgc gctgcttctg ccttgcttaa cggcgccggt gcttccacaa
     7021 agcacgcaag cgctgatctc cggccgggtg tataaccgcg acatcggatt cggcgtcgcc
     7081 ggtgcggtgg tcaccgccac gcataacgcc tccggcctgc ggcaacgggc ggtgaccggc
     7141 gccttcggtt actattggct gccgtcgctt tccgccggaa cctattccat tacggtcacg
     7201 gcacaggggt atcagccgcg cgcggctttc gatgtcaccc tctttaccgc cggttttctt
     7261 accctcgatt tcccaatgcg cgcgctcccc gcggcggcct ggcacggaga atatgtgccc
     7321 ggctacctgc cttccaccga cgccatcgtc cacatctatg cggcggatgt ggaaacgacg
     7381 gcggctgagc cgttgagcgc gctcaagagc cggcagggcg tggccgaggc cagccaatcg
     7441 tacgtgatcg cgcccgagga gatcgaacgc ctgccgctgg caggacgcga cgtctatgcg
     7501 accgtcgccc tgcaaccggg agtgacttca caaccggcga gcgcccgtgg tctgggcctc
     7561 tcggcgaacg gccagcggcc tacatcctcg aatttcctgc tggacggcgt acagatcaac
     7621 gacccgttgg taaccggtcc gttgacactc atggcgccgg aggccgttca ggagtatcgc
     7681 gtctcggtgg gaacgtcttc ggctgaatat ggaggagctg ccggcctcat tgccaacgcc
     7741 gtttccaaag cgggaacgaa tgattggcat gcgctcgctt actggaacgt gcggcatgat
     7801 gcgctcaatg cccaggactt cgaacagaag cggcttggtt acaacaagcc gccgcttcgc
     7861 gagatgcagg agggcttcgt agcgggaggc ccactttcgc ccggccgcgt gttcgggtcg
     7921 ctcgctttcg acgcggttcg attcagaagc cgccgcgatc cggttccgta ttggttgcct
     7981 tcggcctcct tcctcgcttc gctacccgac ggcagcgccg ccggacgcct gctgcggcaa
     8041 tatcccgcgg tggcggttcc gagtgctgga gcgggtatgg cacaaatcga gctggcgccc
     8101 accgcgtcca tcaaccgcta cctggcggcg ccgcgtgctg acgccatcct ggacggggga
     8161 gcccatcaca ttatgagccg ggtcgcgatc gcccgccttc atcagccgga cctgctgtac
     8221 agcccgtacg aacagttcaa cgcaccgtac aaccagaacc tgttgaatgt ggccgccgcc
     8281 tggacctgga gtgcgagccc ccggctgacg aacgaacttc gcgcgggcta cggagagttg
     8341 gaagcggacg tcgatcgtcc acacccggaa gtgcccgtcc tgcttgcgcc acgagtaaca
     8401 ctgcccggca cgcagagttt ctatgccttc caaaccaagg ccagttctgt tgaagtgtca
     8461 aacaccaccg ctctcgcctg gggccgccat ctctttaaat ggggcggctt ctacctgaac
     8521 cgtcgcatag acaactttac gtcgatgggc cgggacggga tctactattt cgattcgctt
     8581 gacgattttg cggcggggaa ctcctccgcg cttctcggtt cggtggcccg cgggcttcag
     8641 ggtctctccg aacccggcta tagacgccgc tatggatatg gtaacgccgc gcttttcgca
     8701 caggatagct ttcgtcctca cccgcggctg acgctcagct tcggcgtgcg atacgaacgc
     8761 ttcggcacac cgaagagcct gcgcgatgca acggacttcc ttgtggcgtg gggacaaggc
     8821 aatgacgttt tcgaacggct gcagggtgca cagcttgcca ggcgcggcgg cgagcacgac
     8881 atgttcgaag gccagcccca tcactgggct gttcgctttg gaatcgctca tgaaattctc
     8941 ccccgccgcg gcaccgtgct gcgcgccggt tacggcattt tctacgaccg cattttcgac
     9001 aacctttggg tgagtgccgc gaccaatgcc gttgcgttcg gcttctggca tttcggtcac
     9061 ccggtggatg tgctgttgcc gctcgcgttc aacaaagcca tggcggccga tttcgcgccc
     9121 acggatctgc tcgactatgt tgcattcgcc ccgcagtttt ccgccggccg cgcgcacaac
     9181 tttttcacgt cggtacagca gccgctgacg cgttccgttt gggtggaagc caccggcgcc
     9241 ggttcactcg gccggggcct cgtcaccacc gatcgcatca atcgctatgg cagtgtgcct
     9301 cgcactccgg ataatctcga acagcgcatt gcgccgggcc ttccgcaact ggcttaccgt
     9361 gcgaaccagg gcgactcgga ttactttgcg ggaacggccc tggttcgttt ccgcgcagcg
     9421 aacatcaccg gacaagtagc ctacacgctc agccatatga ttgatgtgca gtccgatccg
     9481 gtagccgacg attacctgga cctcaactgg ttccgccgcg cgcagacggc cgcgccgcgc
     9541 ccggtccctt ctttcacacg gcaatttcgc cccggcctgg accgcgcaaa ctcggatttc
     9601 gatcagcggc acaacctggt gttcttcgcc gcgctcgatg ttccgcgcgt cgagcggttg
     9661 aagtggctgg ctcgggattg gcgcatctcg ctattaggtg cggtgcgatc cgggacgccg
     9721 tatacggtct atgtgagcgc tcccggaaca ggcgagttga tcgtcaataa ccggcccgac
     9781 ctcatcgcgc cggccgcggt gcgggatggt agcagtgtga atggtggccg caggttgctg
     9841 aacccgtccg cgttccaatt ccccgccgat ggacaggtcg gctcgaccgg ccggaatgct
     9901 ttcgtaggac cggggcagtt cagcttcgat ttttcggtcg cccggcgcct gccattccgc
     9961 tttggagacc ggtcctgcgc cgtgacgttc cgggccgatc tctacaacgc gctgaaccac
    10021 gcaaatcccg gcaaccccct gaacaccacg ctgccgcatc cgcagttcca gtctccgaat
    10081 gattccgaat ttgccgtggc ccgttacgga cgcagaggca gggccgcggg ggtgccgctc
    10141 ctggtgccgc tgtcggaaac tgcgcggcag atacaactca tgctccgatt tgaattttag
    10201 attcgacggt taacacttcg cgcctgtacg ccgtctctcc aacagaattc gccggcgaat
    10261 cgaacccgaa tggagaagac aatgcgtaat ccgatccgaa cctttttcgc ggccgccctg
    10321 ttgttggcgt ccgccacaca gttaccggcc ggcggacttt tcgtgatgct gggcaatcca
    10381 gaagctagcc ccgaggctcg caaacacaat gccgtgttga cgctcacact cgccggttgt
    10441 cacgaacccg aaaaagtgcc ggttgaaggg acggccatca ccgtcgtgga cgggaagcgg
    10501 cagacggtcc cgattcagtt gatcgccctt tccaaacccg gcatgtacgc cgtcacacgg
    10561 cagtggcccg cgaagggccg ttgggtgctg caattcgtag ccaacgacca gggccgcgcc
    10621 gccagcacac tggtagcggc cacgccgggc aagatcgaac ggcactccgc gaaaatggcg
    10681 atgcgcttac catcggagga ggacgtcgcc gcactactct ccggcgagga caagcccgaa
    10741 gtcgcccgca aatgacggca atgactgccc ggcttatgcc actgacgttc gcactccgga
    10801 ctccggccgc gcttgcattc gaccacgcgg ccagagttcg ggcagcagcc ggcggagacc
    10861 gggccgcatt cgacgagctg tatattcgct acgcgagcat ggtgcacggc atcctgctcg
    10921 cgcgtgtccc ggtgtttgtg gcagaagacc tggtgcagga tgtattcctc attgccctcc
    10981 ggaaactttc cggcttgcgc gcgcccgaag cgtttccggg gtggctggcg gctatcgcgc
    11041 gaaaccgcgc cgtggaatat caccggagcg cgcggcacga atccgatgtc cacgccgagg
    11101 ccgcgcgaag gcaggtgcag gcaccggcga gcaatgccct ggatgtgctc agggtcattc
    11161 gtaagctgcc cgaggcgtat cgcgaaacac tgattctcag gctggtggaa ggaatgacag
    11221 gtcccgagat cgcagagcaa accggcctta cgcccgattc cgtccgcgta aacctttttc
    11281 ggggaatgaa aatgctccgc gaagaactcg ggggaggaac aaagtgaacg attatctctg
    11341 ggacggcagt ggccgtcccg aacccgaaat cgaggctctg gagcgcacac ttgccccttt
    11401 gcgttttccg gtcgcgaagc gcccgctcca attgccgcac tcgtcgagac aggccattcc
    11461 ctatgcagca atcgccgcaa gcattgttct cggattggta gtctggcagc tctttcccac
    11521 acacccgcca caactcaccg cgtggacagt gtcggaagac ggggacgccg ccgccgtaag
    11581 aaaggtcctg gtcggagaac gtcttaaaac cggcgaatca tcacgcctcg atttggagtc
    11641 cgatggtttc ggacggatcg agatccggcc ggattccgag atgcgctttc tggaatccgc
    11701 cgccggacgg cagcgcatgg agctgaaaca cgggagtatc cacgctttca tctgggcgcc
    11761 tccacagcaa ttcatcgtgg acactccttc cgccacggca gtggatctcg gctgccagta
    11821 cgagctttcg gtagacaagt cgggcaatgg ctttcttacg gttcagaccg gctgggtcgc
    11881 tttccagctc ggcgatatcg aatcgttcat tcctgcaggc gccgcttgcc ggaccactcc
    11941 cgccggcggc ccgggcagcc ctttcttcga ggacgcttcc atcgcgttcc ggaacgcgct
    12001 cgaggggttt gagcgctttg gcgatcaaaa gatgctggac cgggttctgg tcgaatcccg
    12061 ccctcgcgac gggctgacgc tatggcacct gctggcgaga gtccgcgaaa cggagcgtgc
    12121 agcggtattc gaccggtttg cccaactggt gcccctgcca gccggtgtcg cgcgcgaccg
    12181 cgccatcgcg agagaccgcg ccacgctcaa cctctgctgg aacgctctcg aactcgacag
    12241 caccgactgg tggcggactt ggaaacagca gtggcgcggg cggcgctgag ttcgcgatgc
    12301 cagaatccat ggacttagtg cgtcagctta tttggccgcc gcagcacgtc tgaggaatgc
    12361 ggcgacatcg gtcaggccag ctcgttcggc agcatcgagt ggcgtctgtc cctccgagtc
    12421 tttaatcgtc agatccgcgc cgagtgcgac aagggcttcg atcgctttca tctttcccat
    12481 cgctgctgcg atgtgaagcg gcgtttgaac ctcgtatcga gtacgagcgt tcacatccgc
    12541 cccttgcttc acaagttccc gaatgacctc cgcgttatca cctaactcca atactgttca
    12601 cttaatgatt ttgatagatg cggagaagtc agttttgatt gtcgactgcg gcattcgcgg
    12661 ccgcaacgga tagttgctca tctttcgctt cacacctcgc gggttgatgc gattgcggct
    12721 ggaactgcag cgctcctgaa ggatctcgtc gagaatcgct tcatgaagtg cttttctgtg
    12781 cggagggggg aatagcggcg aagcgggcca tgcgccgctg gacaacacgc accgtgtgga
    12841 gaaacgagag gcggtccgga tcttcgtcag ccttcagggc ggcctcgtgc atcaggccgc
    12901 gaatcgcaaa gtgtgccatc agcaagccat agaattcctg tttgaccagt tccggcgtct
    12961 tgcttcgcag cacgatctga gccccgcgta aatgagtctt cagttcatcc agcgcagttt
    13021 ctatctccca acgctcatgg taaagcgcgg ccaattcttt ggcgggcgcc agtttgtgat
    13081 ccaggatggt ggtgatcagg cgataaacgg gctcggcatc cgggaccttg tccaggcggt
    13141 agtcgatcac acgcaccacc atcgccttac gctcattgcg gcggtccgaa gtggacgggt
    13201 agatgcgact cagataagaa ccgtccggca gtcgctcatc gatctccaga cgcgcattct
    13261 ggcgagtccg ccacagcaga tccgctcccg tagccgcagc tttttgccaa agcttgtggc
    13321 tgggaaagaa ccggtcggcc aggcatagca ttcccttctg cagcgcgggc acaacctctt
    13381 cggccaaggt cagttcatcg gtggcgtact tgtccactcg tgccgcccaa agcacatggg
    13441 ttccgttctc cagcaatgcc gcaaagcgga tcttgggaaa ggccgcgctt ccgcgactgg
    13501 cgccgggtcg cccaaatgct tcctcgtttt ccttcgtgtc cgccacatcc agcgtacttc
    13561 cgtcgagact cgtcagatgc cattgccgat accaggcgcc tttggttcgc ttcccggcaa
    13621 tcggcgccac cattgcttcg tagagcgcct gcataggttc aactcccaaa cggctgcgcg
    13681 cttgcgaaat acccgatttg ccggcgactt tcaccgaagc cgatgggttc agcaaccact
    13741 gcactccttc cagcaaacag cgaaggacct cccgatagga cgaccgcatg tacaacgcta
    13801 aagcgatgac gtaatacacc accacgtgag ccggcaggtc gcgctcacgc aggctggcac
    13861 gtccggtctg ctccagtaca gcatctacct tttcccgtgg gaagtatttc gctatgacac
    13921 ccagactgat gtagtcggtt atacgactgc ctgcgggcaa cgatgcgacg gtacgagcca
    13981 ttctgggggc ctcccgccct aaacagaata gca
//
LOCUS       DASTMK010000387         8564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_242927, whole genome shotgun sequence.
ACCESSION   DASTMK010000387 DASTMK010000000
VERSION     DASTMK010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242927"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFQ79_16800"
     CDS             complement(<1..325)
                     /locus_tag="VFQ79_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141384.1"
                     /translation="MTVPVPIVSNQRETRPSLPDYLPARMVNEFVYCPRLFFYEWVEG
                     LFRESVDTQEGKIQHKRVDREGGALAAPEDLGGEKIHSQSVTLSSDRLRLIAKMDLLE
                     VENGIV"
     gene            complement(327..617)
                     /gene="cas2"
                     /locus_tag="VFQ79_16805"
     CDS             complement(327..617)
                     /gene="cas2"
                     /locus_tag="VFQ79_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEU0141385.1"
                     /translation="MRSSYLVCYDISDDKRLRKVFQTMRGYGDHLQYSIFECQLTPMD
                     LVRCRAELAGIIHHGEDQVLFVNLGPAEGRGDRVITALGKPYTSMDAPCIVV"
     gene            complement(628..1542)
                     /locus_tag="VFQ79_16810"
     CDS             complement(628..1542)
                     /locus_tag="VFQ79_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141386.1"
                     /translation="MSSGVVLGGLDGSNPLAFLAALGALRLSASFEPDVRLHWRMEGE
                     WHPVLTGVAGTEEEELCRRLAGADPIADETLKILGKNLTVPPDVFELFVKTAERNLLE
                     TRDRRAADFAAALGSEVCVDSRKNRIEYTSFCFITGSGHQDFVETARALVQNTKPEQI
                     REALFGPWQCTDKGLSMRWDPSDAVEYALRWDNPGPLGVKSVWGATRLAVEALPLFPA
                     HPTWRGLATTGFREESDLEEFTWPIWETPIGLYTVRTILSLAELRQKTPDRLTLRERG
                     ISEVFRSRRVRIGQGANFKVSFRPARSV"
     gene            complement(1539..4406)
                     /gene="cas3u"
                     /locus_tag="VFQ79_16815"
     CDS             complement(1539..4406)
                     /gene="cas3u"
                     /locus_tag="VFQ79_16815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HEU0141387.1"
                     /translation="METGGEDIVNGPDPEDFSAFFRELHGREPFPWQRRLAMRVCETN
                     WPDVIDLPTASGKTACIDIALFALASRERAPRRIFFVVDRKVIVDDAYHRMLHIREKL
                     AAASDGVLGRVAARLRELGGGEPEPVRVYQLRGGIYRDESWVRNPLQPTVVASTVDQV
                     GSRLLFRGYGVSDYALPVHAGLIANDALILLDEAHCSKAFAQTLERIQNYRGEGWTRR
                     RLEAPFRFVEMTATPSRKPSESPFRLDSEDRVHPLLQERLTAPKQVRFVVSKVKSGDS
                     RRLAVALVAEAQKLVSQDPSILRVAIMVNRVDTARCAFELLKTSHAKLVIGRMRAIDR
                     EDLAERLKPLKSDSKRGPDDPRMFVVATQCLEVGADLDFDALVTECASMDALLQRFGR
                     LDRLGEFGKARAVILAPALDSRKLDAVYGEALAKTREWLEQLAGESGEVDFGLEGAPA
                     TVPQRWKDLPEEERRAMTPPAPCTPVLLPAHLDTLVQTSPRPDPEPAVEFFLHGREPA
                     SADVQVVWRADLDGSDPRKWKDIVKLSPPVQAEALPVKLWAFRRWLRGEKEIVLQSDI
                     EGETPPEEPVRRGRHTNASRPILVWRGEESTSDPAKIRPGDTIVLPGSAEDSDLLGYV
                     AGQRDVGDHAFLEVRGRVRLRLHKALIEMWSKESAPERVLAISRDPDPDWDLLQEALA
                     TYQAEAGDKMPPWLRRSFDGVIGRRRKEFHVLPYPAGGIPGWVLEGKRQLRGPDVMEE
                     DAGADEKSAGEPVPLYTHTEHVRRAARTTAECLLDAAHTEALELAALWHDTGKSDERF
                     QTLLHGADAMAARFAPKLLAKGRFLPQSRVWEHWDRSGLPKGFRHELISLLLAGAKDL
                     PGDHRDLILHLVASHHGRCRPLAPVVLDKSCGEICFDGMTLSPEERRRNAPHKLDLGV
                     SQRFWDLTHAYGWWGLAYLEALFRLSDWKSSLEEQRKGEAE"
     gene            complement(4379..5800)
                     /gene="csb2"
                     /locus_tag="VFQ79_16820"
     CDS             complement(4379..5800)
                     /gene="csb2"
                     /locus_tag="VFQ79_16820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HEU0141388.1"
                     /translation="MTVIGIRYLTGYAAAADLAAGGREPEWPPHPGRVFMALAAAHFE
                     SGEDPTERRALEWLEAEPAPAMRASGATHRSFVETYVPVNDQRYAILNRSRQPRSFPK
                     ARPEEGTVFLVWPSQPPQELRQALAVLCTKVTRIGHSSSLVQLWMPEQLPEPGCNWRP
                     EDGASSLRMRVPAQGTLRSLESAFQARERPRLNAWKGYGKWSTAEDREHTQSGPFDPD
                     FIVMTAMEGRTLGLESTLQLTSALRNAAMKAAGDNPPEWLTGHTPEGAPSRELHAAFF
                     PLPFVGHAHADGHVMGLGIALPRSLSESAEDRRSLREKLGPLFFDPDTGEPRKVRLWR
                     DGVWEWNLERETRESPPRTLQREKWTGPSRMWASVTPVVLHHYPRKRAGDVERILLDA
                     FASALLPEPEALEVRAASAHEGAGHSRSLPSFAEGDPALCRYQTHVIVRFRDPVEGPV
                     LVGRGRYRGYGLFAPWRPGERIS"
     gene            complement(5797..7002)
                     /gene="cas7u"
                     /locus_tag="VFQ79_16825"
     CDS             complement(5797..7002)
                     /gene="cas7u"
                     /locus_tag="VFQ79_16825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HEU0141389.1"
                     /translation="MPDILTFAQLQAAVAGKAAAFRSVTKLQPVGGEGDKIFPATYAG
                     GVYATEKRRLPGESQEVECVLLNSVQSEANHAEEALRQAMLAGEIALPLIEVNFSAAN
                     ENFRKPLPNLSSLEVPHRLADAILRDSNLPDGTRFSKSEYARRWSRANLWNATPVYEL
                     CPTALVFGMWGSPEKPGGLGAKFERAYVSEIVAVDTNNNDKARRSGFRIDPLEVSKSV
                     LVRSNEEGFDLVTDAKAKGALRPSELNHGNIPYESPNTGIRCRYAEQTTVVSLGALRK
                     LRLPVDGRQNPKLDNMGRTVLAAIGLCAGVLAAERGTSLRSRCHLWPVETRKWELLET
                     PGEPPRTFELTGSSVIALLKEAVAAARAMGLNWMDEKLTLTPAPELVELVKQSQEVAA
                     KETGEGEAS"
     gene            7162..7581
                     /locus_tag="VFQ79_16830"
     CDS             7162..7581
                     /locus_tag="VFQ79_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HEU0141390.1"
                     /translation="MSRKQKPPKQPAPPLGVWAPVVALAWVIPGGGHLYLKRNARGAV
                     LMASVLVTFVIGLLMRGMLFQPETGDLLTTIIYTGGFIGNLAAGLPYFLTVWLGYAQP
                     DVAGMVHDYGTKFLVAAGLMNVLAMVDAFEIAARRKD"
     gene            7581..7763
                     /locus_tag="VFQ79_16835"
     CDS             7581..7763
                     /locus_tag="VFQ79_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141391.1"
                     /translation="MKVNLSHFEAALLLALLSSVVLGVVTKKTDPERLRYGLRCFGYF
                     MLALFGIGWLMYLGHG"
     gene            7884..8153
                     /gene="rpsO"
                     /locus_tag="VFQ79_16840"
     CDS             7884..8153
                     /gene="rpsO"
                     /locus_tag="VFQ79_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU0141392.1"
                     /translation="MALAADVKSEVISTHARHEKDTGSTEVQVAILSQRILQLTGHFK
                     DHKQDFHSRKGLLTMVAQRRRLLDYLKSRSPKRYQDLIQKLGLRR"
     gene            8370..>8564
                     /locus_tag="VFQ79_16845"
     CDS             8370..>8564
                     /locus_tag="VFQ79_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141393.1"
                     /translation="MTHTVEIDIEGRVITLETGKIAKQANGSVVVRSGESVVLATACS
                     EEQPKPGAGFFPLTVDYREYT"
BASE COUNT         1647 a         2712 c         2478 g         1727 t
ORIGIN      
        1 tgacgatgcc gttttccact tcgaggagat ccatcttcgc aatcagccgc aaacgatcgc
       61 tcgacagcgt aacggattgt gagtgaatct tctcgccgcc caggtcttcc ggcgcggcaa
      121 gtgctcctcc ttcgcgatcc acgcgcttat gctggatctt gccttcctgc gtgtccacac
      181 tttcgcggaa cagcccttcc acccattcgt aaaagaacag ccttggacag tagacgaact
      241 cattcaccat gcgcgccggc aggtaatcgg gaagcgaagg ccgcgtctcc cgctgattcg
      301 aaactatcgg aacaggaacg gtcatgtcac accacgatgc acggcgcatc cattgacgtg
      361 tacggtttgc ccaaagccgt aatcacgcga tcgccacgcc cttccgccgg gcccaggttc
      421 acaaacaaca cctgatcttc cccgtgatgg atgattcctg ccagctccgc cctgcaacgt
      481 accaggtcca tgggcgtcaa ctggcattca aagatggaat actgcaaatg gtcgccgtag
      541 ccgcgcatcg tttgaaagac cttgcgcagc ctcttgtcat cggaaatgtc gtagcacacc
      601 aggtaagaag aacgcatcag gactctccta cacggaccgc gccggccgga agctgacttt
      661 gaaattcgcg ccctgcccga tgcgaacccg ccgcgagcgg aacacttcac tgatacctct
      721 ctctcgaagt gtcaggcggt ccggagtctt ctggcgcaac tctgcaagcg aaaggatcgt
      781 gcggacggta tagaggccga tgggagtttc ccaaatcggc caggtgaact cctccaggtc
      841 cgattcttcc cggaagccag tggttgccag cccccgccac gtgggatgcg ccggaaacag
      901 cggcaacgcc tccacagcca aacgggtggc accccacacg cttttgacgc cgagaggacc
      961 gggattgtcc catcgcagcg cgtactccac cgcgtcggac ggatcccatc gcatggacaa
     1021 acctttatcg gtgcactgcc aaggcccgaa aagcgcttct cggatctgtt cgggtttggt
     1081 gttttgcacc agcgcgcggg cggtctcaac gaaatcctgg tgaccggatc cagtaatgaa
     1141 acagaagctg gtatattcaa tccggttctt cctggaatcc acgcacactt cggatcccaa
     1201 tgcggctgcg aaatccgccg cacgccggtc ccgtgtctct aacagattac gctccgccgt
     1261 tttgacgaag agttcgaaca catcgggtgg cacggtcagg tttttgccaa ggatcttcag
     1321 cgtctcatcg gcaatcggat ccgcaccggc aagccggcgg cacagctctt cctcttcggt
     1381 gccggccacg cccgtcaata cgggatgcca ctcaccctcc atccgccagt ggagtcgaac
     1441 gtccggctcg aacgaggccg acaggcgtaa cgctcccagg gcagccagaa acgcaagcgg
     1501 gttggatccg tccaacccgc cgagaacgac gccgctgctc attcggcttc tcctttccgt
     1561 tgctcctcca ggctgctctt ccagtcggac aggcggaaga gcgcttccag atacgccagg
     1621 ccccaccagc cgtaggcgtg tgtgagatcc cagaaccgct gtgaaacacc gagatccagc
     1681 ttgtgtggag cattccgccg ccgctcctcc ggcgataaag tcataccgtc gaagcagatt
     1741 tctccgcaag atttatccaa aaccacaggc gcgagcggac ggcaccggcc gtggtgactc
     1801 gccaccagat gcagaatgag atcccggtga tcgccaggca agtccttcgc cccggccagc
     1861 agcaacgaaa tcaattcgtg gcggaatcct ttggggagcc cgctacggtc ccagtgctcc
     1921 cacacacggc tttgcggtag gaagcgcccc tttgcgagca gctttggtgc gaatcgggcc
     1981 gccatcgcat ccgcgccatg gagcagcgtc tgaaagcgct cgtcggactt gccggtgtca
     2041 tgccacagcg ctgccagttc caacgcttcg gtgtgagctg cgtcaagcag acattccgcc
     2101 gtagtgcgcg ccgctctacg aacatgctcc gtgtgagtat agagaggaac cggctcacca
     2161 gcgctcttct cgtccgcgcc ggcatcttct tccatcacat ccgggccgcg gagttgccgc
     2221 tttccttcca aaacccaacc tggaatccca ccggccgggt aaggcaaaac atggaattct
     2281 ttccgccgtc ggccgatcac gccgtcgaac gatcggcgaa gccacggggg catcttgtca
     2341 ccggcttcgg cctggtaggt tgcgagagcc tcttgaagca ggtcccaatc ggggtcgggg
     2401 tcccgggaaa tcgcgagcac ccgctcgggc gccgactctt tggaccacat ctcaatcaat
     2461 gctttgtgca gccgcagccg gacacggccg cgcacctcaa gaaaagcgtg gtcgcccacg
     2521 tcccgctggc cggcaacata accgagcaga tcgctgtctt ccgcgctgcc cggcaggaca
     2581 atggtatcgc ccggacgaat cttcgcggga tcgctcgtgc tttcctcgcc ccgccacacc
     2641 aggatcggtc ttgacgcatt cgtgtgccgc ccgcgccgca ccggttcttc gggaggcgtc
     2701 tctccttcga tatcggattg aagaacgatc tctttttcgc cacgcagcca gcggcggaac
     2761 gcccacagct taacggggag cgcctcggcc tggacgggcg gagagagctt gacgatatct
     2821 ttccattttc gcggatcgct tccgtccaga tccgcccgcc agaccacctg tacatctgcg
     2881 ctggccggtt cgcgtccgtg cagaaagaat tccacggcag gttcagggtc agggcgtgga
     2941 gaggtttgca ccaacgtgtc gagatgcgcg ggcagcagga ccggtgtgca cggagcgggc
     3001 ggcgtcatcg cacgtcgctc ctcttccgga agatccttcc accgttgggg cacagtagcg
     3061 ggcgcgcctt cgaggccgaa atcgacttca ccgctttccc ccgcgagttg ttcgagccat
     3121 tcgcgtgttt ttgcgagagc ctccccatac acggcgtcca gctttctgga atcgagcgcc
     3181 ggagccagga taacagcccg ggcttttccg aactcaccca gccggtcgag acgtccgaag
     3241 cgttgcagca atgcgtccat gctggcgcat tcggtcacca gcgcatcgaa gtcgaggtcg
     3301 gcccccacct ctagacactg tgtggctact acaaacatac gagggtcatc cggcccgcgt
     3361 ttcgagtccg acttcaacgg cttcaagcgt tcggcaaggt cctcccggtc gatcgcgcgc
     3421 atgcgcccga tcaccaactt ggcgtgagat gttttcagca gttcgaaagc gcagcgcgcc
     3481 gtgtccacgc ggttcaccat gattgccacg cggagaatgg acgggtcctg cgaaaccagt
     3541 ttctgtgctt cggcgaccag cgccacagcg agccttctgc tgtcgccgga tttgactttc
     3601 gacacgacga agcggacctg cttcggcgcc gtaagcctct cctgaagcaa tgggtgaacg
     3661 cgatcttcgc tgtcgagccg gaatggcgat tcagacggtt tgcgggatgg tgtcgccgtc
     3721 atctccacaa agcggaaggg cgcttccaga cggcgccggg tccaaccctc gccgcgatag
     3781 ttttgaatgc gttcgagcgt ctgtgcaaac gccttcgagc agtgcgcctc atccagaagg
     3841 atcagcgcgt cgttcgcgat cagccccgca tgaaccggca gggcgtagtc gctgacccca
     3901 taaccgcgga agagtagccg cgagcccact tgatccactg tgctcgccac cacggtcggc
     3961 tgaagaggat tgcgcaccca gctttcgtcg cgatagattc cgccgcgtag ctgatagacg
     4021 cggacgggct ccggttcgcc gccgccgagt tcgcgcaaac gcgcagccac acgtcccaga
     4081 accccatcgc ttgcggccgc cagtttttcc ctaatatgca gcatccggtg ataggcatca
     4141 tcgacgatca ccttccggtc gacgacaaag aagatccgcc gcggcgcacg ctcgcggctg
     4201 gccagtgcaa acagcgcgat gtcgatacag gcggtcttcc cgctggctgt gggcaggtcg
     4261 attacatcag gccagttggt ttcgcacacc cgcatcgcaa gccggcgctg ccaggggaag
     4321 ggctcgcggc cgtgcaactc gcgaaagaac gcgctgaaat cttcgggatc tggtccgttc
     4381 acgatatcct ctcccccggt ctccatggcg cgaacagacc gtagccgcgg tagcggcctc
     4441 gtcctacgag cacgggacct tctaccggat ctctaaaacg cacgatcaca tgcgtctgat
     4501 agcggcaaag cgccgggtcg ccttccgcga aggacggtaa cgagcgcgag tggcccgctc
     4561 cttcgtgcgc ggacgcggcc cgcacttcca acgcttccgg ctcgggcaga agggccgagg
     4621 cgaatgcgtc caggagaatg cgctccacgt cgccggcacg cttcctcggg tagtgatgca
     4681 gaaccaccgg tgtgacgctg gcccacatgc ggcttggtcc cgtccacttt tcacgttgca
     4741 gcgtgcgcgg cgggctctcc cgcgtctccc gttcaagatt ccactcccag actccgtcgc
     4801 gccataggcg cactttccgg ggttcgcccg tatcgggatc gaaaaagaga ggccccagct
     4861 tttcacgtag tgaacgccgg tcttccgccg attccgaaag gctgcgtggc agcgcgattc
     4921 caagacccat cacgtggccg tcggcatgcg catgtcctac aaaaggcagt ggaaaaaaag
     4981 cggcgtgcaa ctcacgcgag ggtgcgcctt ccggtgtgtg cccggtgagc cactccggcg
     5041 gattgtcccc cgcagctttc atcgccgcgt tgcgtagtgc gctggtgagt tgaagcgtcg
     5101 attccagacc aagtgtcctg ccctccatcg ccgtcatcac gatgaaatcg gggtcgaagg
     5161 gaccggactg agtgtgttcc ctatcctcgg cggtgctcca ttttccatat cctttccatg
     5221 cgttcaatcg cggccgttcc cgcgcctgga acgccgattc aagggaacgc agcgtgcctt
     5281 gtgccggtac gcgcatcctc aggctggacg cgccgtcctc aggccgccag ttgcagccgg
     5341 gttcgggtag ttgttccggc atccacaatt ggaccagcga ggaggaatgg ccgatgcgcg
     5401 tcaccttcgt acaaagaacg gccagggcct ggcgcaactc ctgcgggggt tgcgagggcc
     5461 acaccagaaa cacagtgccc tcttcaggcc gcgctttcgg aaaacttcta ggttggcgcg
     5521 aacggttgag tatggcatag cgctggtcgt tcacgggaac ataggtttct acaaacgacc
     5581 ggtgcgttgc gccggaagca cgcatcgccg gagcgggttc ggcctccagc cattcgagag
     5641 cgcggcgctc ggtggggtct tcaccacttt caaagtgggc ggcggccagc gccatgaata
     5701 cacgcccggg gtgcggaggc cattccggtt ccctcccgcc tgccgccagg tcggctgccg
     5761 ccgcatagcc ggtcaggtac cgaataccaa taacggtcat gacgcttcgc cttctccggt
     5821 ctctttcgcc gctacctcct ggctctgctt caccaactcc accagctccg gcgcgggagt
     5881 gagtgtcagc ttttcgtcca tccagttgag tcccattgcc ctggcggccg cgacggcctc
     5941 cttcaagaga gcgatgacag agcttcccgt taactcgaat gtgcgcggcg gttctcctgg
     6001 cgtttcgagc agctcccact tgcgagtctc tacgggccag aggtggcagc gcgaacgaag
     6061 gctggtaccc cgttccgcgg ccagaacccc ggcgcacaat ccgatggcgg cgagcaccgt
     6121 gcgtcccatg ttgtccagtt tcggattctg ccgcccgtcg acggggaggc gtagcttccg
     6181 caaagcgccg agggagacca cggtggtttg ttcggcgtaa cggcaccgga tgccggtgtt
     6241 gggactttca taaggaatgt tgccgtggtt taactcggag ggccgcagcg cgccctttgc
     6301 cttcgcatcc gtgaccaggt cgaagccttc ctcgttggaa cgaacaagca cacttttgct
     6361 gacttcgagc gggtcgatgc ggaatccgct tcttcgtgct ttgtcgttgt tgtttgtgtc
     6421 taccgccacg atttccgaca cataggcccg ctcgaacttg gctcccaatc cacccggttt
     6481 ctccggcgat ccccacatcc caaacaccag cgccgtgggg catagctcgt agaccggcgt
     6541 ggcgttccac aagttcgcgc ggctccaccg cctggcgtac tccgatttgg agaagcgtgt
     6601 gccgtccggc agattcgagt cgcgcaggat ggcgtcggcg aggcggtgcg gaacctccag
     6661 gctactgagg tttggcagcg gtttgcgaaa attctcattg gcggcggaga aattcacttc
     6721 gatcagcgga agcgcgattt cgccggcgag catcgcctgc cgcagcgcct cctcggcatg
     6781 gttggcctcc gattgcacag agttcaggag aacacattca acttcctgtg actccccggg
     6841 caggcggcgc ttttcggtgg cataaacacc gcccgcgtaa gtggcaggaa agattttgtc
     6901 tccttccccg cccaccggct gaagttttgt aacggaacgg aaggcagctg cctttcccgc
     6961 aacagccgcc tgaagctggg caaaagtaag tatgtctggc attagattcc tctggtgtac
     7021 ttgtatgtgc gagcaggccc tccgctcacg aaaaattaca aagtttaaat cagtatttta
     7081 tgtctgaaaa taccgtcgtg gggatgggcc ggtaccggta ctccgcctct ccaaacgatg
     7141 ttgttacact attagaaagc tatgtcccgg aaacagaagc cgccgaaaca accggcgcct
     7201 ccgctgggtg tgtgggcgcc cgtcgtggcg cttgcctggg tgattccagg gggcggccac
     7261 ctgtacctga agcggaacgc cagaggagcg gttctgatgg cttcggtgct tgtcaccttt
     7321 gtaatcggac tcctgatgcg cggaatgctc ttccagccgg agaccggcga cctgttgacc
     7381 accatcatct atacaggtgg attcattggc aatcttgccg ccggcttacc atactttctg
     7441 acagtatggc tggggtatgc ccaacccgac gtagcgggta tggtccacga ctacgggaca
     7501 aagtttctgg tcgccgccgg cctcatgaac gtcctcgcca tggtggatgc gttcgaaatt
     7561 gccgcacgaa ggaaggactg atgaaagtca acttatccca cttcgaagcg gctctcctgc
     7621 ttgcgctgtt gtcgtccgtt gttttgggag ttgttacgaa gaagaccgat cccgagcgcc
     7681 tgcgctacgg gctgcgctgc ttcggttact tcatgctcgc gctgttcggc attggctggc
     7741 tgatgtatct cggccacggt taggttattg atttcgcctg ggtgtcagga cgtcccccgg
     7801 ctgtccactg gacattaccg ccggtgtggt atgctattcc tgtacagaaa agattaaggc
     7861 aagacaagga gtatccactc tagatggcat tggcggctga cgtcaaatct gaagttatct
     7921 ctacccacgc acggcatgaa aaggacacgg gctctacaga agtccaagtg gccatcttga
     7981 gccagcgcat cctgcagcta acgggtcatt tcaaggacca caaacaggat tttcactccc
     8041 gaaagggcct gctcacgatg gtggcccaac ggcgccgcct gttggactat ctcaagagcc
     8101 gcagtcccaa gagataccag gatttgattc agaagttagg cctccgtcgc tagaggggtt
     8161 ctcaggtgcg agcgggcaac aggcccttcg ctgtccaacc actcctgtct gttctcggga
     8221 agcattggac atcgccctcg ttgaaaactc gcaaggtgat gccactccga tgagtggctc
     8281 cggcccggtt cgggctaccc tccaagtatc cactccagtc tccttgatac gccggccgga
     8341 tcggtttccg gccgccaaag gaattccaga tgacacatac agttgaaatt gatatcgagg
     8401 gtcgcgttat cacccttgaa accggcaaga tcgctaaaca ggcgaacgga tccgtggttg
     8461 tccgatccgg agagtccgtc gtccttgcaa cggcgtgttc ggaggagcag cccaaaccgg
     8521 gcgcaggttt ctttccgctc accgtcgatt accgtgagta taca
//
LOCUS       DASTMK010000388         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_82714, whole genome shotgun sequence.
ACCESSION   DASTMK010000388 DASTMK010000000
VERSION     DASTMK010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82714"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VFQ79_16850"
     CDS             complement(<1..1238)
                     /locus_tag="VFQ79_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU0141394.1"
                     /translation="MAARIMIYAVVIIAVVVALAGVTLWKTLALKNRADFLVAGRKLP
                     WTVLVFTLLSSWIGAGSLFAGGENAYRNGFAALWQPAGGWLGLIVIAFIAGRARRFAQ
                     FTVPDLLEKRYNAAARVLGTVAIVISYTVITSYQFKGGGDIMHMIFPDMDRTTGMYIV
                     ALFVIVFTAAAGMASVAYMDLVIGALVTVIVVIAVPLVLSSAGGWSGVRASLPDTHFQ
                     VLGNLSLSQALGFTIPTMLLLVGNQGMYQKFFSARSERDAKLAVGGWIVGTLLLETLL
                     IALAVIGSAKFKTEAPREIIPITARLGLPTVIGAILLGGIFAKIISTANNYLFSPATN
                     LIHDVYERFIDRNASDRRSLIVSRIIVVLLGLFALLQASHFESILRAALYAYTVYGAA
                     VTPAVLAVFFWRRTTTAGAIS"
     gene            complement(1220..2425)
                     /locus_tag="VFQ79_16855"
     CDS             complement(1220..2425)
                     /locus_tag="VFQ79_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0141395.1"
                     /translation="MVVAQNLSKLFRLYDRPSDRLFEALPFRSKRLHTNFWALKDINL
                     HVQRGETFSIIGPNGSGKSTLLQILCGVLTPTSGRVAVRGRIAALLELGAGFNPEFTG
                     RENVYLNGEIMGLARAEIDASFHSIEAFAEIGEFIDRPVKEYSSGMYVRLAFSTAIHV
                     RPDVLVVDEALAVGDAIFSNRCVRKFEELKAAGVTVIFVSHDLSLVKRLSDRAMLMAH
                     GKSIAEGTPSDVVNRYIGMVHERNLIDDHGTRRELGAASFRHGDSASRILDVSVMDGA
                     GNPTNVLETGSPMRVRVRAQFQRAVSEPVIGILLRNRLGTDVFGTNTRCEGVRLGNFA
                     EGEIAEVDFAFDCLLSRQTYTLTAATQYWDGMSQDWLDDVVSFSVVERKGVAGVACFS
                     TSIEWRRAS"
     gene            2483..>2939
                     /locus_tag="VFQ79_16860"
     CDS             2483..>2939
                     /locus_tag="VFQ79_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0141396.1"
                     /translation="MDFRLATPDDYSVLEPMVVDGFEPITWVKMIDEKYGPLNGVNWR
                     ERWKKRFRGIFDSQTVLIGEIDGKIVACATGTLDAETKLGYIDILAVAPGEQGKGYGR
                     TMLRGMLDYFRAHGAEHAHLECLVWNDAGNCLYRSEGFEEVARSIRWFVK"
BASE COUNT          670 a          855 c          888 g          526 t
ORIGIN      
        1 gagatggccc cggcggttgt agtgcgccgc cagaaaaaga ctgccaatac tgcgggtgtg
       61 acggcggcgc cgtagaccgt gtacgcatag agcgcggcgc ggagaatcga ttcgaaatgg
      121 ctggcctgca gcagcgcgaa caacccgagc agaaccacga taattcgcga cacgatcagg
      181 ctgcggcggt ccgaggcgtt ccggtcgatg aagcgctcgt agacgtcgtg aatcaggttc
      241 gtcgccggcg aaaacaggta gttgttggcc gtggaaataa tctttgcgaa gatgccgccc
      301 agcagaatgg ctccgatgac ggtaggcaac ccaaggcgcg cggtaatggg gatgatctcg
      361 cggggcgcct ccgttttgaa ctttgcgctc ccaatcacag ccagggcaat cagcagtgtt
      421 tccagcagca gggtgcccac gatccagccg ccgacggcga gtttggcgtc gcgttcggaa
      481 cgtgctgaaa aaaacttctg atacatgccc tggttgccca ccagaagcag catggtagga
      541 atggtgaaac cgagcgcctg agagagcgat agattcccga gtacctgaaa atgcgtgtcc
      601 ggcagactgg cgcgaacgcc gctccaaccg ccggccgatg acagcaccag cggcacggcg
      661 attactacaa tcacggtgac cagcgcacca atcaccaggt ccatataggc aaccgaggcc
      721 attccggcgg cggccgtgaa cacgatgacg aacaacgcga caatgtacat gcctgtggtc
      781 cggtccatgt ccggaaagat catgtgcatg atgtcgccgc cacctttgaa ctgatagctg
      841 gtgatgacgg tgtatgagat gacgatggcg acggtcccca gcactcgcgc ggcggcgtta
      901 taacgcttct ccagcaggtc gggcacggtg aattgagcga agcgccgggc gcgtcccgcg
      961 atgaaggcga tcacaatcag gcccagccag ccgccggcgg gctgccagag cgcggcgaat
     1021 ccattgcgat aagcattttc tccgccggca aacaggcttc ccgctccaat ccaggaagag
     1081 agcagcgtaa acacgaggac cgtccagggg agcttgcgcc ccgcgacgag aaagtccgcg
     1141 cgattcttga gcgcaagcgt tttccagagc gtcacccctg cgagagcaac aaccaccgcg
     1201 ataattacta cggcatagat catgatgcgc gccgccattc gatagaggtc gaaaaacagg
     1261 caactccggc aacgcccttg cgctccacaa cggaaaagga gacgacgtcg tcgagccagt
     1321 cctggctcat accgtcccaa tactgcgtgg cggcggtaag ggtataggtc tggcgtgaca
     1381 gaaggcagtc aaacgcgaaa tccacttccg cgatttcgcc ttcggcgaag ttccccaggc
     1441 gcacgccttc acaccgggta ttggttccga atacatcggt tcccagccgg ttgcgcaaca
     1501 gaatgccaat gaccggttcg gaaacggcgc gctggaattg cgcgcgaacc cgcacccgca
     1561 tcggagatcc ggtctccagt acattggtgg ggttgcccgc gccatccata acggaaacgt
     1621 cgaggatccg gctggcgctg tcgccgtgcc ggaaagaagc ggcgcccaac tcgcgccggg
     1681 taccgtgatc gtcgatcagg ttacgctcat gaaccatccc gatgtaccgg tttacaacat
     1741 cgctgggagt cccttcggca atggactttc cgtgcgccat tagcattgcc cggtcggaga
     1801 gccgttttac cagcgacagg tcgtgcgaaa caaagatgac ggtgacgccc gccgctttca
     1861 actcctcgaa ctttctgacg caccggttgg agaaaatggc gtcgcccacc gccagggctt
     1921 cgtcgaccac cagcacgtcc gggcgcacgt ggatcgcggt cgagaaagcc agccgcacgt
     1981 acatgccgct cgaatattct ttgacgggcc ggtcgataaa ttccccaatc tcggcaaacg
     2041 cttcaatgga gtggaacgat gcatcgattt cggcgcgcgc gaggcccatg atttcaccgt
     2101 tcaggtaaac attttcccgg cccgtgaact ccgggttgaa gcccgctccc aattcgagca
     2161 gcgcagcaat gcgccccctc accgcgacgc gccccgacgt cggagtcaac acaccgcaca
     2221 gaatctgcaa aagggtactc ttgcccgagc cgttgggacc gatgatgctg aacgtctcgc
     2281 cccgttgaac atggagattg atatccttca gcgcccagaa attcgtgtgg agccgcttgg
     2341 atcgaaacgg cagggcttcg aacagcctgt ccgaaggacg gtcgtaaagg cggaatagct
     2401 tggaaaggtt ctgggctaca accacgatga atcgacaata tagcgcattc aaagcatgac
     2461 ccggcgtgat acggtggaaa gcgtggactt caggttagcg acgccggacg actattctgt
     2521 tttggagccg atggtcgtag acggcttcga acccattacc tgggtgaaga tgatcgacga
     2581 gaagtacggg ccattgaacg gcgtgaactg gcgcgagcgt tggaagaagc gctttcgcgg
     2641 catattcgat agccagaccg tcttgatcgg tgaaatcgac ggaaaaattg tagcgtgcgc
     2701 gaccggcaca ctggatgcgg aaacaaagct cggctacatc gacatccttg cggtggctcc
     2761 cggcgagcag ggtaaaggct acggccgcac aatgctgcgc gggatgttgg actactttag
     2821 agcgcatggg gcagagcacg cccacctgga atgcctggtt tggaatgatg cgggcaactg
     2881 cctctaccgc tcggaaggct tcgaagaagt tgcccgctcg atccgttggt tcgtcaagt
//
LOCUS       DASTMK010000389        15790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_21117, whole genome shotgun sequence.
ACCESSION   DASTMK010000389 DASTMK010000000
VERSION     DASTMK010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15790
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21117"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFQ79_16865"
     CDS             complement(<1..808)
                     /locus_tag="VFQ79_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEU0141397.1"
                     /translation="MKSVTGHEIRQKFLDFFASRGHRVVRSASLVPAEDPTLLFTNAG
                     MNQFKDVFLGLEKRDYTRAASSQKCVRAGGKHNDLENVGYTSRHHTFFEMLGNFSFGD
                     YFKREAIRYAWDLITVDYGLPKDRLYVTVFREDDDAEQLWQEVAGVPKDRIFRLDEKD
                     NFWQMGETGPCGPCSEIHYDLGPEAAEPGRADKPFPSDAGGRYVEIWNLVFMQYDRAT
                     DGTLTPLPRPSIDTGMGLERIAAVLQGKLSNYDTDLIHPIVLRAAELFHVT"
     gene            900..1421
                     /locus_tag="VFQ79_16870"
     CDS             900..1421
                     /locus_tag="VFQ79_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU0141398.1"
                     /translation="MARTAQKGEDMGMAIEDMKDSLRQRGIRLTRQRQILLDLIDKSG
                     QHLDAEGLYQMAKERDPKLNRVTVYRTLKMLKEGGLVDELDLMHYGGDQHYYETRLKQ
                     EHAHIICLRCGKVEEFFGEPLQKLRRQVEAHFGFQILLARTEIGGYCSHCQVLRAKEV
                     KDLTAVSAGARRG"
     gene            1421..3103
                     /locus_tag="VFQ79_16875"
     CDS             1421..3103
                     /locus_tag="VFQ79_16875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU0141399.1"
                     /translation="MVSAKRGPAPPGVLTVVNPSGNRTRIPVQPLPFRIGRHSDNDLV
                     LRDNRVSRIHAQVSIEEEEFWIEDLKSRHGVFVNGSRVQGRQKLGNADRIDFGIRDSY
                     HLVFNIEDAELSRLLDQISPPGTGTSQGAGNLAKLRALVEVARVMQSSLSTQDVLTAV
                     VDAALAVTGSERGFLLLKEQDELEVRVARHRRGMSLPATELRVPTRLIHRALTQRREL
                     LSMSFDPNDEAGVRPDISVADLELSSVVCLPLVRIRTGSNEDTRIISSAHETAGMIYM
                     DSRCGPADLSAGNRELLQTLALEASTILENARLLEQERAKLKMEEELNVARAIQQGLL
                     PKALPHAGWFQASGSSIASHQVGGDYYDVRQIRENAWCAVVADVSGKGVSSALLASLL
                     QGAFLLASEGQVEIQEMMNRINHFLNERTQGDKYATIFYCVLDANGNLQWANAGHCAP
                     LLLRTGGEIEELKPTGMPVGLLEMASYSVEDTRLNRGDKLILYTDGFSEATNEAGEFF
                     EQKRLRELLRVNARLNSAELHQAMMRAVEEFTDGAEQSDDITMVVLEYASVS"
     gene            3145..3969
                     /locus_tag="VFQ79_16880"
     CDS             3145..3969
                     /locus_tag="VFQ79_16880"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU0141400.1"
                     /translation="MSFQSMRVISLESRRSAEMSQLIRNQQGEPVQAPSMREAPMERN
                     EEVYRFGERLFAGAFDMMILLTGVGTRLLNKALAERYPEERFAEALRGITTVVRGPKP
                     AAALRELGVTPTVFVPEPNTWRELLASVDERPEKRIAVQEYGRTNDELIAGLRSRGAA
                     VETVRIYRWELPEDTGPLEHAVKLIANGEAQVLLLTTSIQIAHLFQVAERMGLAGEVR
                     RELPRMVIASIGPTTTESLEEFGFHPDLEPSHPKMGFLVKETAQRAPELLEAKRER"
     gene            3959..4183
                     /locus_tag="VFQ79_16885"
     CDS             3959..4183
                     /locus_tag="VFQ79_16885"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU0141401.1"
                     /translation="MNDRRNWIRLTGEYLSLAFLLPSATFVGYAIGYFLDRTFGTSFL
                     YLVFLLLGIAGGFIQLVRQIQKDSRPRRGL"
     gene            4173..4550
                     /locus_tag="VFQ79_16890"
     CDS             4173..4550
                     /locus_tag="VFQ79_16890"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEU0141402.1"
                     /translation="MDSDSALAARAHRRIGRIMMILSIAGVAAAFSIYGTAAGAGFLA
                     GAAMSVVNFRWLKQSVDSLGPGAGRKRGAVLFAIRYAVFGATAYVIVKYFEVSLQALL
                     IGLLVSIAAVIIEIVYELIYARA"
     gene            4537..5274
                     /gene="atpB"
                     /locus_tag="VFQ79_16895"
     CDS             4537..5274
                     /gene="atpB"
                     /locus_tag="VFQ79_16895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU0141403.1"
                     /translation="MPEHELWFTALFNDYLAGAGNAILNIFGLQAEHPERPWANFITM
                     QILVALIIIVLFAILRTRLSMDKPGKLQHFFEVIYEFLANQTDEVVGHNGRRYLAFFG
                     TLFIFILFANLLGIIPTLESPTMFPYVPVGCAIAAFLYYNVVGIKEVGVGGYMKHFVG
                     PMPLMAPLMIPIEIISHLARPLSLSIRLFANMYAGEQVTLVFLGLTYVVAPALFMGLH
                     VFIALLQAYVFVLLTMVYVGGAVSHEH"
     gene            5369..5668
                     /locus_tag="VFQ79_16900"
     CDS             5369..5668
                     /locus_tag="VFQ79_16900"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU0141404.1"
                     /translation="MNVKLFLFLLAMFVLASPVFAQETAGGGTNWVAITSGFSMAIAS
                     GLCGLAQAKAVAASAEGMARNPSAAAAIRFALLLGLVLIESLALYTLVIIFAKVV"
     gene            5711..6595
                     /locus_tag="VFQ79_16905"
     CDS             5711..6595
                     /locus_tag="VFQ79_16905"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0141405.1"
                     /translation="MKLEGILPPIATPFDHEGNLYKAKLKHNVEKWNRTGLAGYVVCG
                     STGESVYLTTEEKIQLWEWVAEFAAPGKLLLAGTGMESVRETVWLTNKAAEIGYKAVM
                     VRTPHYYKNLLNRADAQCVYFRAVADASKIPLMIYNWPQATGVDIPAEAVVALSEHPN
                     IIAIKESSGNIEKVMQMIREVKPGFQVLVGSAPTLWPSFAVGAVGAVLAYANAAPYST
                     IAIWEAHRTRENDAARDWQNRIARASWLVTVKHGIPGLKYAMDLNGYYGGPTRLPLVP
                     PSPEAKQEIEEAFRDLKG"
     gene            complement(6752..8104)
                     /locus_tag="VFQ79_16910"
     CDS             complement(6752..8104)
                     /locus_tag="VFQ79_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141406.1"
                     /translation="MSKSGWSRRHFFYGSLLAGAVPAAGFGSTPSLKHLGYKSPNEKL
                     NIAAIGAGGKGYSDLKGCETENIVALADPDEKRAARTFNEYPQVPKYKDFRRMLDKEE
                     KNIDAVTVSTPDHTHAIASMWAMARGKHVYCQKPLTRTVWEAQQLKEAAAKYKVATQM
                     GNQGYSNDGARLCAEIIWNGDIGNVTEVHAWTNRPLKYWPQGPDVVPQEAAVPSTLDW
                     DVWLGMAKYRPYSPAYAPHHWRGFPDFGCGAIGDMACHILGTPNMALRLGNPVSVECI
                     KQEGKGEYTFPQKTVIRFDFAARGSMPPVKLFWYDGMTAQPEVEGVPESERLGDKDVN
                     GSLFVGEKGMVTTGCYGEKTRLVPAWKMLDYKLPPEFLTRSPGHYRDWIRACKGGQAS
                     CSDFSVAGPFVEWMLLGVIAMRFEGKLEWDANKGRFTNNKEANKYLKPYIRDGWSFNG
                     "
     gene            complement(8172..8954)
                     /locus_tag="VFQ79_16915"
     CDS             complement(8172..8954)
                     /locus_tag="VFQ79_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEU0141407.1"
                     /translation="MNLLFVGDIFASPGRSIVAEHLQRIVAEEHIDLALANAENAAGG
                     FGITPMIAEELFSCGLDVLTSGNHIWDKKEIYEYLCRQPRLLRPANYPSDLPGKGVYV
                     AEARNGARCAVINLQGRTYMPATECPFRKADQILQALNPSVKVRIVDFHAELTSEKMA
                     MGWHLDGRVSGVFGTHTHIPTADTRILENGTAYQTDLGMTGPYDSVIGVQKDIVIRRF
                     LTAMPVRMEAAKRGAELHAAILSVDEETGKATSVRRYSIEQR"
     gene            complement(9210..9854)
                     /locus_tag="VFQ79_16920"
     CDS             complement(9210..9854)
                     /locus_tag="VFQ79_16920"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU0141408.1"
                     /translation="MATDLIEIDSEQPSPDALEKAAAALRRGEIIAIPTDGLYSIVAD
                     PLNLQAVGRVFSAKGRQPHQSLPLIVSDLLMAQELAKEASTRFYLLARHFWPGPLTII
                     VPASEKVPLKVTGNTGRLGLRHSGSRVATALVEWLAQPLIATSANISGQPTCRSGIEV
                     FGMMDGRIDLVLDGGLCTGPGSTTVDVAEPYWRVIREGAVSEKEIADCLAVGGL"
     gene            complement(9873..11753)
                     /locus_tag="VFQ79_16925"
     CDS             complement(9873..11753)
                     /locus_tag="VFQ79_16925"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEU0141409.1"
                     /translation="MTTSTPPIPKSRAIEFRKRLQEKVLVADGAMGTMLYSRGVFINR
                     CYDELNVSAPAMVKEIHEQYVKAGAEVLETNTFGANRMRLGAFGLAEKLKAINEAGVR
                     LAREAAGESAFVAGAIGPLGARIEPLGRTSFSEARDIFAEHARALTEAGIDLLLLETF
                     SDLNELREAALAAREAAGPDMVIVAQVTIDDYGNLTGGTTTEQFTCRMDKFPVDALGL
                     NCSAGPRVMLETIEKMAKFSAKPLSAMPNAGHPSTVEGRKIYLCSPEYMAQYARRFLW
                     AGVKIIGGCCGTTPEHIKAIRSEARSLQPAIRKIAVTVETPAENQKIFKNVPVAEKSK
                     LGAKLAAGRFAAFVEILPPRGVDASKEIAGAKACAEQGIDCINVPDGPRASARMSAQV
                     TCQLIQQHAGIEAVLHFCCRDRNILGIQSELLGAHAVGIRNLICITGDPPRMGGYPDA
                     TAVFDVDAIGLTNIVSNLNRGLDIGGNPMGSQTQLLIGVGANPGAFNLEEELRRFEWK
                     VEAGAEYAVTQPVFDLNLLEQFLKKTGHLHIPFIAGIWPLTSYRNAEFMVNELRVPVP
                     EQFMERMRRAESAEQARQEGVLIAQEMVQKVRPMVAGVQLSAPFGRYQMAIEVAQAIG
                     AR"
     gene            complement(11759..12568)
                     /gene="mutM"
                     /locus_tag="VFQ79_16930"
     CDS             complement(11759..12568)
                     /gene="mutM"
                     /locus_tag="VFQ79_16930"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU0141410.1"
                     /translation="MPELPEVEAVSRRLREQVTGAEIRRARVLRPRSTYPQPPSKVEH
                     LAAGRTIEAVQRRGKHILIYLSGGIVLGCHLRMTGNLHVIPDAGTRPASTRLYLELAD
                     GRGIVLNDPRALGVMNAFPLAQAEVLMSKLGIEPLSAEFTPDRLIEFAARSKMPVKVF
                     LMDQRFISGLGNIYAAEALYRARIHPVTPASQIRPVRLRRLHAAIVDVLNDAVKSAYI
                     AYERPGQFREAERFPLAVYGREGVCCSVCHRKIRRLTQAGRSTYYCPGCQR"
     gene            complement(12585..13373)
                     /locus_tag="VFQ79_16935"
     CDS             complement(12585..13373)
                     /locus_tag="VFQ79_16935"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU0141411.1"
                     /translation="MPRSIITLTTDFGTSDHFVGTMKGVLLSIAPQAQLVDITHDLNP
                     FEISEGAFVIAQAYGYFPKNTVHLVVIDPGVGTARRPILAQAGGQFFVAPDNGVLSMV
                     YSREKPKVRAVTADKFFLKPVSNTFHGRDVFSPVAAHVARGASPAAFGKLIKDYLRLD
                     LETPFRTGKRVWTGTILKIDHFGNLITNFRMDEFTDIQTRRFEMAVGTRKLWRLANTY
                     AEVEPGELVLIAGSAGFVEICANQASAAKLLGCGVGAPAELTIY"
     gene            complement(13481..15697)
                     /locus_tag="VFQ79_16940"
     CDS             complement(13481..15697)
                     /locus_tag="VFQ79_16940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEU0141412.1"
                     /translation="MSARIANLPVLLLIAFSQAATGQLTFWSTQSQAAVGPRNQAIAQ
                     RIASDHQGNTYVAANLLQDVTFPSALSNAPELRYDVALIKFDSTGKEVFQAELRGGGN
                     DTVAGLAVDSAGNAYLTGFTNSTDFPVFRPYQREHRGESGSAFVAKISAAGSTEYVTL
                     LGGSRGATGSAIAAGLDGSAYITGRTSSTDFPVTEGAFQKEGPPYDFFATFSPRTYAF
                     AAKLSPDGSRLVYSTYLGGDTARCSGGSNCIGVVGGAAGLAIAADAAGNAYMAGNTNA
                     TNFPVTPGALQTMCRCIRRAGDVFVSKLNPTGTALVYSTYLGGDTVGSEPYTDGDESL
                     VSIALDPNGVVAVTGVTRAATFPVTADALKLRTDYRDDMRQPPADGFLSLLSADGASL
                     LYSTLIGGGAEESIVALRRGPAGTYFLSGTTHSTDFFRTTGFARGSDFLIEFDPVQRQ
                     VLRAQRWPTGVVGGDMAVSEQGAVLLAGGNSGVLTRLLPGDSNQPQILGIASAAGSRT
                     TAAVSPSELVSLYGVDLGPGEPRFLELSEGRRVTTELGGVQVLFDGIPAPLLYAQRDQ
                     INAVVPFEMAGRESVSIQVRTATAGMTPAFQVTIAEATPELFRDSPDTANFLRLFALN
                     QDGTPNSSRNAAARGSIIALFGTGAGRLEPTPENGMLATEPLSQAALPVRVLMNGRPC
                     EILYAGAAPTLVAGMLQVNARVPEGLSAASDVQSVHVQLFVGDRQTERGYISVGPP"
BASE COUNT         3058 a         4684 c         4841 g         3207 t
ORIGIN      
        1 aggtgacatg gaacaactcc gccgcgcgca gcacgatggg atgaatcagg tccgtatcgt
       61 agttcgataa tttgccttgc agaacggccg cgatgcgttc caatcccatg ccggtatcga
      121 tggaaggccg cggcagcggt gtgagcgtgc cgtccgtggc gcggtcatac tgcatgaaca
      181 cgagattcca gatctcgacg tagcggcccc ccgcatccga tgggaagggt ttgtccgccc
      241 gtcccggttc cgcggcttcg ggtcccaggt cgtaatggat ttccgaacag ggaccgcagg
      301 ggccggtctc tcccatctgc cagaaattgt ccttttcgtc gaggcggaag atgcggtctt
      361 tcgggacgcc ggcaacctcc tgccagagtt gctccgcgtc gtcgtcctcg cgaaagacgg
      421 tgacgtacag acggtccttc ggcagtccgt aatcgacggt aatcaagtcc caggcgtaac
      481 ggatcgcctc ccgcttgaaa tagtcgccga aggagaaatt cccgagcatc tcaaaaaacg
      541 tgtggtgccg gctggtatag cctacgttct ccagatcgtt gtgcttccct ccggcacgga
      601 cgcacttctg cgagctggcg gcgcgcgtgt agtcgcgttt ttcgagcccc aggaacacgt
      661 ccttaaactg attcatgccg gcgttcgtaa acagcagggt ggggtcctcg gccggaacca
      721 aagaggcgct gcgaacgact cgatggccgc gggaggcaaa aaaatcgagg aatttctggc
      781 ggatttcgtg acctgtcaca gatttcattt tgacacgagc gcgcccggac agcccgccgg
      841 gtgccggagg acgctccggc accgggcctt cagaatctgg cgcaaacctc acaataagaa
      901 tggcaagaac ggcccaaaaa ggcgaagata tgggtatggc gatcgaggat atgaaagact
      961 ccctcaggca aaggggcatc cggctgacgc gtcagcggca gattctcctc gacctgatcg
     1021 acaagtccgg gcagcacctc gatgccgagg gcctgtacca gatggccaaa gagcgtgatc
     1081 cgaagttgaa ccgcgtaacc gtctaccgga cactcaagat gctgaaggaa ggcggtctgg
     1141 tggatgaact cgacctcatg cattatggtg gggaccagca ctactatgaa acgcgcctca
     1201 agcaggaaca tgctcacatc atttgcctgc gctgcggcaa agtggaggag ttcttcggag
     1261 agcccctcca gaagctgcgc aggcaggtgg aggctcattt cggatttcag attctgctgg
     1321 cgagaaccga gatcggcgga tactgctctc attgccaggt gctgcgagcg aaggaagtga
     1381 aggacctgac ggctgtttcc gccggcgccc ggagaggctg atggtttccg caaaacgcgg
     1441 accggctccg cccggggtac tgaccgtagt aaatccgtca ggcaatcgca ctcgcattcc
     1501 agtgcagccc ctgccgttcc gtatcgggcg ccactccgat aacgaccttg tactgcgcga
     1561 caaccgggtc tcgcgtatcc atgcccaggt ctcgatcgaa gaagaggagt tctggatcga
     1621 agatctcaaa agccggcatg gcgtcttcgt aaacggcagc cgcgtgcagg gccgccagaa
     1681 gctgggaaat gccgaccgca tcgatttcgg cattcgcgat tcctaccatc tggtcttcaa
     1741 catcgaagat gccgagctca gccgtctgct cgatcagata tcgccgcccg gcaccgggac
     1801 gtctcagggc gcgggaaacc tggccaagct gcgtgcgctg gtcgaggtgg cgcgggtgat
     1861 gcaaagctcg ctgtcgacgc aggatgtgct gacggcggta gtggatgccg cgctcgccgt
     1921 aaccggttcc gagcgcggat ttctcctgct caaagagcag gacgaactgg aggtccgcgt
     1981 ggcccgtcac cgccggggta tgtcgcttcc cgcgacggag ctgcgcgttc ccacccgcct
     2041 gatccatcgc gcgctgacgc aaaggcgcga gctgctgtcg atgagcttcg accccaacga
     2101 cgaggcgggc gtccgcccgg acatttcggt tgccgatctg gagctgtcta gcgtggtctg
     2161 cctgccgttg gtgcgcattc gaaccggatc gaacgaggat acgcgaatta tctcttccgc
     2221 ccacgagacc gcggggatga tctacatgga ctcccggtgc ggaccggcgg atttgtccgc
     2281 cggaaaccgc gagcttttgc agacgctcgc acttgaagct tctacgattc tcgaaaacgc
     2341 ccggcttctc gaacaggagc gcgccaagct gaagatggag gaggagttga atgtcgcccg
     2401 cgccatccaa caggggctgc ttccgaaagc gctgccccac gccggctggt ttcaggcgtc
     2461 cggctcgagc atcgcttcac atcaggtggg cggagactac tacgatgtgc gccagatccg
     2521 cgagaacgcg tggtgtgcag tggtagccga tgtttcggga aaaggcgtca gctccgctct
     2581 gctggcatcc ctgctccagg gtgcgtttct tctggcttcc gaaggccagg tagagattca
     2641 ggaaatgatg aaccgtatca atcatttcct gaatgaacgt acccagggag acaagtacgc
     2701 caccattttc tattgcgtgt tggacgccaa tggcaatctg caatgggcca atgcggggca
     2761 ctgcgcgccc ctgctgctca gaacaggcgg agagattgaa gagctgaaac cgaccggaat
     2821 gccggtaggg ctgctggaga tggcatccta cagcgtcgag gacacgaggc tcaaccgcgg
     2881 tgacaagctg attctctata cggacgggtt ttcggaggcc accaacgaag ccggcgaatt
     2941 ttttgagcag aagcggttgc gcgagttgct tcgcgtcaac gcacgcttga atagcgcgga
     3001 gctgcatcag gcgatgatgc gggcggtgga agaattcacg gacggagccg agcagagcga
     3061 cgacatcaca atggtggtgc tcgaatacgc gtccgtgtcg taatacccga ttcgcctgcc
     3121 gaacgggagg ctatactaac cacgatgtcg tttcagtcca tgcgggtgat ttcgctcgag
     3181 agccgccggt ccgccgaaat gagccagctc atacgcaacc agcagggtga accggtgcag
     3241 gcaccctcca tgcgggaggc gccgatggaa aggaacgagg aggtgtaccg gttcggagaa
     3301 cggctgttcg cgggcgcgtt cgacatgatg atcctgctga ccggtgtcgg aacccggctc
     3361 ctcaacaagg ccttggctga gcgttacccg gaagagcggt ttgccgaagc gttgcgcggg
     3421 atcaccacgg tggtgcgggg gccgaagccg gccgcggcgt tgcgcgagct tggggttacg
     3481 cccaccgttt tcgtgcccga accgaataca tggcgcgagc ttctggcgag cgttgacgag
     3541 cgtccggaga aaaggatcgc ggtgcaggag tatggcagaa ccaacgacga gttgatcgcc
     3601 ggcctgcgca gccggggcgc cgctgtggaa actgtgcgca tctaccgttg ggagctgccc
     3661 gaagatacgg gacccctgga acatgccgtg aagctgatcg cgaacggcga agcgcaggtg
     3721 ctgctattga ccacctccat tcagattgcg cacctgtttc aggtcgccga aaggatggga
     3781 ttggccgggg aggtgcggcg cgaactgccg cggatggtga tcgcctccat tggcccgacc
     3841 accaccgaaa gcctggaaga gttcggcttt catcctgacc tggaaccgtc ccatccgaag
     3901 atggggttcc tggtgaaaga gaccgcccag agagcgccgg aactgctgga ggcgaagcgt
     3961 gaacgataga aggaattgga tccggttgac gggcgaatat ctgtcgctgg cctttctcct
     4021 tccctccgcg acgttcgtgg gctatgcgat cggatatttc ctcgatcgca ccttcgggac
     4081 gtcgtttctc tatcttgttt ttcttctgct cggaattgcc ggcgggttca tccagcttgt
     4141 gagacagatc cagaaggatt cccggcctcg ccgtggactc tgacagcgca ttggcggccc
     4201 gcgcgcaccg gcgcatcggg cgcatcatga tgatactgtc gatcgccgga gtggcggctg
     4261 cgttttctat ctatgggacg gcggccggcg ccggcttcct cgcgggcgcc gcgatgtcgg
     4321 tcgtcaattt ccgctggctg aaacagagtg tcgattcgct cggtcccgga gccgggcgaa
     4381 aacgcggcgc cgttctcttt gccatccggt atgccgtttt cggagcaacg gcttatgtta
     4441 tagtgaaata cttcgaagta agccttcagg cgcttctgat cgggctgctc gtctccatcg
     4501 cggccgttat tatcgagatc gtctacgaat tgatctatgc cagagcatga gttgtggttc
     4561 acggcgctgt tcaacgatta tcttgccggc gcaggcaacg caatcctgaa catttttggg
     4621 ctacaggcgg aacatcccga acggccatgg gcgaatttta ttaccatgca gattctggtc
     4681 gccctgatta ttattgtttt gttcgccatt ctgcgcacca ggctttccat ggacaagccg
     4741 ggaaagctgc agcacttctt tgaagtgatc tacgagttcc tggcgaatca gaccgacgaa
     4801 gtggtcggcc ataacgggcg gcgctatctc gcctttttcg gaacgctgtt catatttatc
     4861 ttattcgcta acctgctggg aatcattccg acgctcgaat ccccgacgat gttcccgtat
     4921 gttcccgtgg gttgcgcgat cgcggcattt ctttattaca acgttgtggg tatcaaggaa
     4981 gtcggtgtcg gcggctacat gaagcatttc gtcgggccga tgccgttgat ggcgcccctg
     5041 atgattccca tcgagatcat cagccacctg gcgcggccgc tttccttgag catccggctt
     5101 tttgccaaca tgtatgccgg tgagcaggtg acgctggtct ttctgggact cacctatgtt
     5161 gtggcgcccg ccctgttcat gggattgcac gttttcatcg cgttgctaca ggcgtatgta
     5221 ttcgtgctgc tgacgatggt gtatgtcggc ggcgcggttt cgcacgagca ttgattcggc
     5281 cttatccgtt cccaatgtga gcccggctgc ttgaggatga cgggaaccac ctcctgggag
     5341 cgattttata cgaaggagag aaagaagcat gaacgtgaag ttgtttctgt tcctgctcgc
     5401 gatgttcgta ctcgcgagcc ctgtgtttgc ccaggaaacg gcgggcggcg gcaccaactg
     5461 ggttgccatc acctccggat tctccatggc gattgcgtcc ggtctttgcg gactcgcgca
     5521 ggcgaaagcc gtagcggcgt ccgccgaagg catggcccgc aacccgagcg ccgcggcggc
     5581 cattcgattc gcacttctgc ttggactggt gctgatcgag tcgctggcgc tgtacacgct
     5641 ggttatcatc ttcgccaagg ttgtgtaatc gcgaaagacg ggcgggccct tccggctcgc
     5701 cctttctccc atgaaactcg aaggaatttt acccccgatt gcgacaccct tcgaccacga
     5761 agggaacctc tataaagcaa aactgaagca caatgtcgag aagtggaacc ggaccggcct
     5821 ggcgggctac gtcgtgtgcg gctccacggg cgaaagcgtc tacctgacca cggaagaaaa
     5881 aatccaactg tgggagtggg tggcggagtt cgccgctccc ggcaagctgt tgttggcggg
     5941 caccggtatg gagagcgtcc gcgaaacggt ttggctcacc aacaaagccg ctgaaatcgg
     6001 atacaaggcc gtcatggtgc ggacgccgca ctactataag aaccttctga accgggcgga
     6061 cgcgcagtgc gtttatttcc gtgcggtcgc ggacgcgtcg aagatcccgc tcatgattta
     6121 caactggcct caggccacgg gtgtagacat tccggcggag gcggttgtgg cgctttccga
     6181 acacccgaac atcatcgcca tcaaggagag ctcgggcaat atcgagaagg tgatgcagat
     6241 gatccgcgag gtgaagcccg gcttccaggt gctcgtcgga tcggcgccaa cgctctggcc
     6301 ctcttttgcc gtgggcgcgg tgggcgcggt gctggcctat gccaacgcgg cgccatattc
     6361 gaccatcgcc atctgggagg cgcaccggac gcgcgaaaac gatgcggcgc gggactggca
     6421 gaaccgcatc gcgcgggcat cctggctcgt aaccgtgaag cacgggattc ccggcctcaa
     6481 atacgccatg gacctgaacg gctactatgg cggtccaaca cgtctgccgc tggttcctcc
     6541 ctcgcccgaa gcaaaacagg aaatcgaaga ggcgtttcgc gatctaaagg ggtagcttgc
     6601 tgtgcggcat cccaaacacc ctcacagagg cgaaagtaga ttggccacgg atgaacacgg
     6661 attcacacgg atcaagcctt gtgtttttct tatctgtgtt catccgtggc caatcaaatc
     6721 gtcgcgcaaa catttatgac gtcgtgttta gttaaccgtt gaagctccat ccgtcgcgga
     6781 tgtacggttt caaatacttg ttcgcctctt tattgttcgt gaagcgcccc ttgttcgcgt
     6841 cccattcgag tttgccctcg aatctcatgg cgatgacgcc gagcagcatc cactcgacga
     6901 aaggccctgc cacgctgaag tcggagcacg aagcctgccc gcctttgcac gcgcgaatcc
     6961 agtcgcgata gtggccggga gaacgcgtca ggaactccgg aggcagcttg tagtccagca
     7021 ttttccaagc cggtacaagc cgcgtcttct cgccgtaaca gcccgtcgtc accattccct
     7081 tttcgccgac gaagaggctg ccgttcacgt ccttgtcgcc cagtcgttcg ctctcgggta
     7141 cgccttccac ctcaggctgg gcggtcatgc cgtcatacca gaacagcttc actggcggca
     7201 tgctgccgcg cgcggcgaaa tcgaaccgga tgacggtctt ctgcgggaag gtatactctc
     7261 ccttgccttc ctgcttgatg cattccacac tcaccggatt ccccaggcgt agggccatgt
     7321 tgggagtccc gagaatgtgg cacgccatat cgccgatcgc gccgcagccg aagtccggaa
     7381 acccgcgcca gtggtgagga gcataggcgg ggctatacgg tctgtatttc gccatgccga
     7441 gccacacgtc ccaatcgagc gtcgaaggga ccgccgcctc ctgcggcacg acgtccggac
     7501 cctgcggcca gtacttcagc ggccggttcg tccaggcgtg gacttcggtg acgttgccga
     7561 tgtcgccgtt ccagatgatt tcggcgcaaa gacgcgcgcc gtcgttcgag tagccctggt
     7621 tccccatctg cgtggcgacc ttgtatttcg cggcggcttc ctttaactgc tgcgcttccc
     7681 acaccgttct cgtcaacggc ttctggcagt agacatgttt gccgcgcgcc atggcccaca
     7741 tcgacgcaat cgcatgagta tggtccggcg tcgaaacggt gacggcgtcg atgttcttct
     7801 cttccttgtc gagcatgcgg cggaagtcct tgtacttcgg aacttgagga tactcgttga
     7861 aggttcgggc ggcgcgtttc tcatccggat ccgcgagcgc aacgatattc tctgtctcgc
     7921 agcccttcag atcggagtag cctttgccgc cggcgccgat cgccgcgata ttcagtttct
     7981 cattcggcga tttatagccg agatgcttta gcgaaggggt acttccgaat ccggcggccg
     8041 gcaccgcgcc ggccagcagg ctgccgtaaa agaaatgccg tctggaccat ccgctcttgg
     8101 acacgctgaa ctcccgcaac gaagatttgg accacagaga gattcatctt atcgcaccgt
     8161 cccgcgagcg tttagcgctg ctcgatggaa taccggcgca cggaagtagc cttgcccgtc
     8221 tcctcgtcca ccgagaggat ggcggcgtgc agttccgcgc cgcgtttggc ggcttccatg
     8281 cgaacgggca tagcggtaag aaaccggcgg atgacgatgt ccttctgaac cccgatcacg
     8341 gaatcgtagg gccctgtcat tcccaggtct gtctggtatg ccgtgccgtt ctcgaggatt
     8401 cgcgtgtcgg cggtggggat gtgtgtgtga gtgccgaaaa cgccggagac ccttccgtcg
     8461 aggtgccagc ccatcgccat cttttcactg gtcagctccg catgaaaatc gacaattcgc
     8521 acctttacgg aggggttcag agcctgtaga atctggtccg ctttacggaa cgggcattcc
     8581 gtggcgggca tatacgtacg cccttgaagg ttgatgacgg cgcagcgcgc cccgtttcgg
     8641 gcctcggcga cgtatactcc tttgccgggc aggtcggagg ggtaatttgc ggggcgcagc
     8701 aggcgcggct ggcggcagag gtactcgtag atctcctttt tgtcccaaat atggtttccc
     8761 gacgttagaa catccaggcc gcaggaaaag agctcttcgg cgatcatggg tgtgatgccg
     8821 aaaccgcccg cggcgttctc cgcgttcgcc agcgccaggt cgatgtgttc ctccgccaca
     8881 atgcgttgaa gatgctcggc gacaatactg cgtccgggag aggcaaagat atcgcctaca
     8941 aaaaggagat tcatgggtat atgtgtggga cgcgggtggg attgccggaa tcggagcgta
     9001 ccgctgggcc gtgtcagcct ccgtcattga ccccgttttt tataacgggg gagccctccg
     9061 agcgtcttca ggcttctcca acgcgttaat ccgcgggagc ttgcacacca acgcatcatc
     9121 gagccgggct ccatgacttg actgttggat caaattatgg agaccgttca cctgctttcg
     9181 acgacacgct cctcgtcgat cataacagat taaaggccgc ccacggccag gcaatcggcg
     9241 atttccttct ctgaaaccgc accttcgcga atgacacgcc aataaggttc tgcaacatcc
     9301 acggtagtac tgccgggccc cgtgcacagg ccgccgtcca gaaccaggtc gatgcggccg
     9361 tccatcatgc cgaaaacctc gatgccgctc cggcacgtcg gctgtccgga gatgttggcg
     9421 ctggtcgcga tcaggggttg ggcgagccac tcgaccagcg ccgttgcgac gcgggagccg
     9481 gaatgccgca gccccagcct ccccgtgttc ccggtaactt tgagcggcac tttctccgaa
     9541 gccgggacga taatggtcag cggcccgggc cagaaatggc gggccagcag gtagaaccgg
     9601 gtcgaggcct ccttggcaag ttcttgagcc atcaggagat cggagacgat caggggaagg
     9661 gactgatgag gctggcgacc cttggccgaa aaaacccggc cgaccgcctg caggttcagc
     9721 gggtcggcaa cgatgctgta cagcccgtcc gtcggaatgg cgatgatttc gccccgcctg
     9781 agagcggctg ccgccttttc cagggcatcg ggactcggtt gttcggaatc tatctcgatc
     9841 agatcggtgg ccactggcgt gagactcgta gtttatcttg ccccaatggc ttgagcaact
     9901 tcgatggcca tctggtaccg gccgaagggg gcgctcagtt gcactccggc caccatcggc
     9961 cggacttttt gcaccatctc ctgagcgatc aacacccctt cctggcgcgc ctgctcggcg
    10021 gattccgccc gccgcatacg ctccatgaac tgctctggta cgggcacccg cagctcattc
    10081 accataaact cggcattgcg atagctggtg agcggccaga tgcccgcgat aaacggaata
    10141 tgcaggtgcc cggttttctt gaggaactgc tccagcaggt tgaggtcaaa cacgggttgg
    10201 gtaacggcgt actccgctcc ggcttccact ttccattcga accggcgcaa ctcctcttcc
    10261 aggttgaacg cacccgggtt cgcgccaacg ccgatgagca actgcgtctg ggaccccatc
    10321 ggatttccgc caatgtcgag gccccggttc aggttgctga cgatatttgt cagcccgatg
    10381 gcatctacat cgaataccgc cgttgcatcg gggtacccgc ccattcgcgg cggatctccc
    10441 gttatacaga tcaggttccg gatgcccacg gcatgtgcgc cgaggagttc ggactggatt
    10501 ccgagaatat ttctatcccg gcagcaaaaa tgtaagacgg cctcgatgcc ggcgtgctgc
    10561 tggatcaact ggcaggtcac ctgcgcgctc atgcgggcgc tggctcgcgg cccgtccgga
    10621 acattgatgc agtcgattcc ctgttccgcg cacgccttgg ccccggcaat ctctttggat
    10681 gcgtccacgc cccggggcgg caggatttcg acaaaggcgg cgaaccgtcc cgccgcgagt
    10741 ttcgcaccga gcttcgattt ctccgcgacc ggaacgtttt taaagatctt ctggttttcg
    10801 gccggcgttt ccaccgtcac ggcgattttc cggatggctg gctggagaga tcgcgcctcg
    10861 gaccggatgg ccttgatgtg ctccggtgtc gttccgcagc agcctccgat gatcttgacg
    10921 ccggcccaca ggaaacggcg cgcgtactga gccatgtact ccggcgaaca cagatagatc
    10981 ttgcggccct ccacggttga gggatgccct gcattgggca tcgcacttag cggtttggcc
    11041 gaaaactttg ccatcttctc gatggtttcg agcatcaccc ggggaccggc tgagcagttc
    11101 agcccgagcg catccaccgg gaatttgtcc atgcggcacg taaactgttc cgtcgtagtg
    11161 ccgcccgtca gattgccgta gtcgtcgatg gtgacctgcg cgacgataac catgtccggg
    11221 ccggccgcct ctcgcgcggc cagggcggct tcccggagtt cattaagatc ggaaaacgtc
    11281 tcgagcagca gcaggtcgat tcccgcttcg gtcaaagcgc gggcatgctc ggcgaaaatg
    11341 tcgcgggctt cggaaaagga agtccgcccg aggggttcga tgcgggcgcc caaaggacct
    11401 atagcgccgg ccacgaaggc ggattctccg gcggcctcac gcgccaggcg gactcccgcc
    11461 tcgttgatcg ccttcagctt ctcagccaat ccaaacgccc cgaggcgcat ccggttcgca
    11521 ccgaacgtgt tggtttccag cacctccgcg ccagctttca catattgttc gtgaatctcc
    11581 ttcaccatgg ccggcgccga aacgttcaac tcgtcgtagc agcggtttat gaagacaccg
    11641 cgggagtaga gcatggttcc catcgcgcca tcggcgacca gaaccttctc ctgaaggcgc
    11701 tttcggaatt caatggcacg ggacttcggt atcgggggtg tggaggtggt catcgatttt
    11761 agcgctggca accggggcaa taataagtgg agcggccggc ttgcgtcagc cggcgtatct
    11821 ttctgtggca gacggaacag cagacgcctt ccctaccgta gactgccagc ggaaaccgct
    11881 ctgcttcccg gaactgccca ggacgttcat acgcgatata ggccgatttt accgcatcgt
    11941 tcagcacgtc aacaattgcg gcgtgaaggc ggcgcagtct caccgggcga atctggctgg
    12001 cgggcgtaac cgggtggatt cgcgcccggt acaacgcctc cgcggcgtaa atgttgccca
    12061 atccgctaat aaagcgctgg tccatcagaa atactttcac cggcatcttc gaccgcgccg
    12121 cgaattcgat caggcgatcg ggcgtgaatt cggcgctgag gggctcgatt ccgagcttgc
    12181 tcatcagcac ctcggcctgc gccagcggaa atgcgttcat cacgccgagc gcgcgcgggt
    12241 cgttcaggac gattccgcgg ccgtcggcaa gctccaggta caggcgtgtg gaggcaggcc
    12301 gggttccggc atcgggaatg acatggagat tgccggtcat tcgcaggtgg cagcccagga
    12361 caatgccgcc cgacaggtaa atcaggatat gcttgccgcg ccgctggact gcctcgatcg
    12421 tccgtccggc tgccaggtgc tccactttcg acggcggttg cggataagtc gagcgcgggc
    12481 gcaggacccg cgcacggcgg atttcggcgc ccgtcacctg ctcgcgaagc cttcttgaaa
    12541 ccgcctccac ttcgggtaat tccggcattg ctcctcttta tgtttcaata gatggtcagt
    12601 tcggcgggcg cacccacgcc gcagcccaac aacttggcgg cggaagcctg gttggcgcag
    12661 atctcgacaa agccggcgct cccggcgatc agaaccaact cgcccggttc cacttccgcg
    12721 taagtattgg caagccgcca gagttttcgc gtacccacag ccatctcaaa ccggcgggtt
    12781 tgtatgtccg tgaactcgtc catacggaag ttcgtgatca gatttccgaa gtggtcgatc
    12841 ttgagaatgg ttcccgtcca tacccgcttg ccggtgcgga agggtgtttc gagatcgagg
    12901 cggagataat ctttgatgag cttgccgaag gccgctgggg aagcgccgcg ggcgacatga
    12961 gccgcaacag gcgaaaaaac atcgcggcca tgaaatgtat tgctcaccgg tttgaggaag
    13021 aacttgtccg ccgttaccgc gcgcaccttc ggcttctccc gcgaatacac catggagagc
    13081 acgccattgt cgggagcgac aaagaactgc ccgccagcct gggccagaat cggccgccgc
    13141 gcggtgccga cgcccggatc gatgaccacc agatgaacgg tattcttcgg aaagtaaccg
    13201 taggcctggg caatgacgaa agcgccctcg gaaatctcga acgggttcag atcatgtgtg
    13261 atgtccacta gttgtgcttg cggcgcaata ctcaaaagga cgcccttcat cgtccccaca
    13321 aaatgatcgg aggtgccgaa atcggtagtc agcgtgataa tggaacgcgg catattcgac
    13381 aagaccgctc gctgacgcgc gcggctccgt aatcgcttct gtgagcggtt ggcatggtta
    13441 ttctgtgaaa gtcccttagt gtttagcata acggcccggc tcagggtggt cccacgctga
    13501 tgtagccgcg ttcggtctgg cggtctccaa cgaagagctg gacatggacg gactggacgt
    13561 cgctagcagc cgatagtcct tccggcacgc gcgcatttac ttgcagcatg ccggccacca
    13621 gcgtgggagc agcaccggcg tataagatct cgcagggccg cccgttcatc aggacacgca
    13681 ccggaagcgc cgcttgagac agcggctccg tggcgagcat accattctcc ggagtgggtt
    13741 ccagccggcc tgctccggtg ccgaacagag cgatgatgga gccgcgtgcg gcggcgtttc
    13801 gggatgaatt cggcgtgcca tcctgattca gtgcgaacag tctgaggaaa ttcgccgtat
    13861 ccggagagtc tcgaaagagc tcgggcgttg cttcggcaat cgtcacctgg aacgccggag
    13921 tcatcccggc cgtggcggtt cgcacctgga tggaaacgga ttcgcgtccg gccatctcga
    13981 aaggcacgac cgcattgatc tggtctcttt gtgcataaag cagcggtgcg ggaatcccgt
    14041 cgaacaacac ttgaacgccg ccaagctccg tggtgacccg ccgtccctca gagagttcca
    14101 gaaagcgggg ctctccgggg ccaaggtcca ctccatagag tgaaaccaac tcgctgggtg
    14161 aaacggctgc tgttgtccgc gagccggccg cactggcaat cccgagaatt tgtggctggt
    14221 tcgaatcgcc gggaagaagg cgggtaagga cgcctgaatt cccgcccgcc aacaggacgg
    14281 cgccctgctc gctgacggcc atgtctcccc caacgacgcc ggtgggccag cgttgcgcgc
    14341 gaagaacctg gcgctgtacc gggtcgaact cgatcaggaa atccgatccg cgcgcgaaac
    14401 cggtcgttct gaaaaaatcg gtggaatgcg tagttccact gagaaagtac gtgccagccg
    14461 ggccgcggcg tagcgcgacg atagactctt cggcgccgcc gcctatcaag gtggagtaca
    14521 gcagcgatgc gccatccgcc gacagcaggc tcaggaaccc atcggccggc ggctggcgca
    14581 tatcgtcccg gtagtctgtt cgcagtttca gagcatccgc ggtgacgggg aatgtcgcgg
    14641 cgcgcgtgac gccggtcacg gccaccacgc cattcggatc gagcgcgatc gaaacgaggc
    14701 tctcgtcccc atccgtgtaa ggttcggagc caaccgtgtc tccaccgagg tatgtcgagt
    14761 acaccagagc ggttccggtg gggttcagct tggatacaaa aacatcgccg gcgcgccgga
    14821 tgcaccggca cattgtttgg agcgcgccgg gggtgaccgg aaaattcgtg gcgttcgtgt
    14881 tgcccgccat gtaggcattg cccgccgcat cggctgcaat cgccaatccg gccgcaccac
    14941 ccacaacgcc aatgcagttg ctgccgccgc tgcaacgggc cgtgtcaccc ccgaggtaag
    15001 tggagtaaac cagtcggctg ccatccgggc tgagcttggc ggcgaaggca taggtgcggg
    15061 gtgagaacgt agcgaagaag tcgtacggag gaccttcctt ttgaaaagct ccttccgtca
    15121 cgggaaaatc cgtggaggag gtccggccgg tgatgtaggc gctgccgtcg aggcctgcgg
    15181 cgatcgcact tcccgtagca ccgcgcgagc cgccgagcag agtgacatac tctgtcgatc
    15241 ccgccgccga gatcttcgcg acgaaggcgc tgccggattc accgcggtgc tcccgctgat
    15301 acggccggaa caccggaaaa tcggtggaat tggtgaagcc ggtcaggtag gcgttgccgg
    15361 cgctgtccac tgcaaggcct gcgaccgtgt cattaccacc gccccgcagt tcagcctgga
    15421 atacttcttt acctgtggaa tcgaatttga tgagtgccac atcgtagcgc agctccggcg
    15481 cgttcgataa ggcggacgga aaggtgacgt cctgaagcag attcgctgcg acgtaagtat
    15541 ttccctggtg gtctgaggcg attctctgtg cgatcgcctg attgcgggga cccactgcgg
    15601 cctgggattg cgtactccag aaagtgagtt gtccggtagc ggcctgtgaa aacgcgatca
    15661 gcaggagcac cggcaggttt gcgatgcgag cggacatggc tgttagaatt gaagtaatcg
    15721 ctgttgtgaa ccgcttttat ttcgaccaca atgctactac tccggtgtct tcggaggtgc
    15781 tcgccgggca
//
LOCUS       DASTMK010000390         5369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_21167, whole genome shotgun sequence.
ACCESSION   DASTMK010000390 DASTMK010000000
VERSION     DASTMK010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21167"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFQ79_16945"
     CDS             complement(<1..518)
                     /locus_tag="VFQ79_16945"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0141413.1"
                     /translation="MPAHPFNTRNGALHVISSEPPPARDRPLALPEPIFPLHPEKCGD
                     VAPDSRSVPQNGSPYRTRVTPFMLRRALRGWLYPYLRSRLLPGDFHPMIAYLFTEWKC
                     NLDCHYCWAFDNRVNGMTENTARRAIDWLHDTGCRVLALMGGEVLLRPKFVHKVVYYA
                     AQKGFWVYVPTNG"
     gene            complement(583..1551)
                     /locus_tag="VFQ79_16950"
     CDS             complement(583..1551)
                     /locus_tag="VFQ79_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0141414.1"
                     /translation="MKLAQIFPAWGMILDGRRPFLSIEVTRECPLRCPGCYAYAPNHT
                     GNAGSLRTLSDLSGKDLIDGILEVTRRLRPLHISLVGGEPLVRYKELDALLPLLAPTE
                     VQLVTSAVRPIPAAWAEHRHLHIVVSVDGLPREHNRRRAPATYERILENIAGHQVIIH
                     CTITRQLMQRAEYLQDFAGFWSGRPEARKIWFSLFTPQAGEFPEERLRPSERQRAIQE
                     LAGLPLLFPKVYTSKPLLDGYAHPPTRPEQCIFARVTTCISSDLKTEIRPCELGGTPE
                     CCECGCIASAGLAAIGRYRLAGLVRVGDIFRASSRIGEMRHGYNAT"
     gene            complement(1617..3005)
                     /locus_tag="VFQ79_16955"
     CDS             complement(1617..3005)
                     /locus_tag="VFQ79_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0141415.1"
                     /translation="MASKLNAALSWLAPVAADPPDPAYRRQQATEVIDGFRVLSLLVC
                     ITAAAMFLMKILQWDSPPASHHTASLAVWAVLAVAGIFGFWASRSIQSPTLALVATAA
                     LLGTSVAAVTMDRLLQSAQDGAPAPSVIPAIFILLMLGAALMPLRPLRVLGLGVLLLL
                     SCAASAAFMGVPLKGSFMDSATALLSVAISVAISARFTTQRIRLYNAHAAAVQSAKET
                     EAARSRALMAESAVTMERLAASLSHELNTPIGALKSATDTLIRGVERHASFPKGSHMP
                     ELVEELSDAIQQSAARLSETIARIQRFANLDRSAIRLTDVNQLIQDAVALMNPPSFNQ
                     AQIVLHLDPVPHVWCKPHRLSAALASILNMGIEGGYAVTIDTYAAAAHVNVEVTITGG
                     REGLPEQQELTPGFGVEAGRVRATGWDLFAARQLVWEIGGDLRIGQTGPGERAITVTI
                     PVTVMAPRQSAA"
     gene            3071..3808
                     /locus_tag="VFQ79_16960"
     CDS             3071..3808
                     /locus_tag="VFQ79_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141416.1"
                     /translation="MQLVKGADDARQAMRTLLLSLVLTGIVRVPVAGANRLPTAVIVV
                     MEGSPGQQGEAASKIVGALKTVLGCRHTLQVARFDAEPGLQSRLTVKGANRDLMAVEP
                     RTPMREAIEAGLIQLITVSSPRTMVVIAHEQFYPTTVSKDRLLELARRSEAKIHTIYL
                     ASNRDKPGVLRRFGRSLRNGIVWLVEVIILEERGYSARDTSHLLKFMADATGGKYCVA
                     TDESSGIVCADGISAAIAAEPQLYSQP"
     gene            complement(4016..5230)
                     /locus_tag="VFQ79_16965"
     CDS             complement(4016..5230)
                     /locus_tag="VFQ79_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141417.1"
                     /translation="MKLPVLLLLLAWLPAVAQQLPSIEEAIAAKNWFYERAVHPETKL
                     IYSRVNLDDPQLWKHVRFATPEAVRGKPNNDSEVPNLSNCAGAGGVFLGQLVDIHAVT
                     GDPQCIRQARAVFSGLESLATASKRPGFIARGLLPGDASRAHFHNSSVDQYTFYVYGL
                     YKYFHSALAGEPEKASMRRIMQDICTMLERDGTILGSNGAPGWVSDIEAIRSDRSSRL
                     LQMYLVGHAITDDPHWRDVYLEKLREGRYARLRTVLDPVQLRYTYTPRDLARGPEHAD
                     IGTIWQTQYSLVPLFELETELPLKTAWLEALQTGARIAERYGSDGPELQIIMLAQNRH
                     VISAVPTPGQLQKNEELKRRVADLISKTPRWQTEMPRGNAFAASLTHFGLTWVGVMDA
                     YWTGILRGGFHP"
BASE COUNT         1100 a         1565 c         1668 g         1036 t
ORIGIN      
        1 ccgttggtcg ggacgtaaac ccagaaacct ttctgtgccg catagtaaac gactttatga
       61 acgaacttgg gccgcagcag aacctctcca cccatgagcg cgaggacgcg gcaacccgtg
      121 tcatgcagcc aatcgatggc gcgacgggcg gtgttctcgg tcatcccgtt tacgcggtta
      181 tcgaacgccc agcagtaatg gcaatcgagg ttgcacttcc actccgtgaa caagtaggcg
      241 atcatcggat ggaaatcgcc gggaagcaat cgggatcgca ggtaagggta gagccagcca
      301 cgaagcgcgc ggcgcagcat gaacggcgtc acacgcgtcc tgtatgggct cccgttctgc
      361 ggtacagatc gggaatcggg cgccacatcg ccgcacttct cgggatgcaa ggggaagatg
      421 ggctccggca aggccaatgg cctgtctcgc gccggcggag gctctgagct tatgacatgc
      481 aaagccccat tgcgggtatt gaatgggtgt gccggcatct ctttcttcga tcctcctcat
      541 tcgtttaaga cggcacatcg tgttccaaaa gctgccagcc ggctacgtcg cgttgtaccc
      601 gtgacgcatt tcgccaattc tgcttgacgc gcggaagatg tcgccgactc gcaccaatcc
      661 cgcgagacga tagcggccga tggcggccaa ccccgccgac gcaatgcatc cacattcaca
      721 gcactccggc gtcccgccca gttcacaggg acggatttcc gtcttcaagt cagacgagat
      781 gcaggtggtg acacgtgcga agatgcactg ctccgggcgt gtgggtggat gcgcatagcc
      841 atcgaggaga ggcttggacg tatatacttt tggaaacagc agaggaagac cggccagctc
      901 ctgaatggcg cgttggcgtt ccgagggccg gagccgctct tccggaaatt caccggcttg
      961 cggagtgaaa agactgaacc agatctttcg cgcctccggg cggccggacc agaacccggc
     1021 gaaatcctgc aaatactcgg cacgctgcat gagttggcgc gtgatggtgc aatgaatgat
     1081 cacttggtgg ccggcgatgt tttcgaggat tcgttcgtaa gtcgcgggcg cccggcggcg
     1141 gttatgctcg cgtggaagcc cgtcaacgga tacaacgatg tgcaggtggc ggtgttctgc
     1201 ccaggcagcc gggatggggc gcacggcact ggtgaccaac tgaacttccg tgggagcgag
     1261 gaggggaagc agagcgtcca gttccttgta gcggactaga ggttcgcctc cgaccaggct
     1321 gatgtgcagc gggcgaaggc ggcgcgttac ctcgaggatt ccatcgatca gatcctttcc
     1381 ggacagatcg gatagcgtgc ggagcgaacc ggcattgccg gtatggttcg gtgcgtaggc
     1441 atagcagccg ggacagcgaa gcgggcactc ccgtgtcacc tcgatcgaca ggaacggccg
     1501 ccgcccatcg agaatcattc cccaggcagg gaaaatttgt gccagtttca tcgggatatt
     1561 cacccctctt tgccgaatat gtcagatgcc atcggcagca gccgattccc ggcagttcat
     1621 gccgcgcttt gtcgaggtgc catgacggtg accgggatcg tgaccgtgat cgcccgttca
     1681 cccgggccgg tctgcccaat tcgcagatct cccccgatct cccacacaag ttgccgggcc
     1741 gcaaacaggt cccatccggt cgcccgaacg cgcccggcct caacaccgaa gccaggagtc
     1801 aactcctgct gttcaggtag tccttcgcgg cctccggtga tggttacctc gacgttgacg
     1861 tgggccgcgg cggcgtacgt gtcgatcgta acggcatatc caccttcgat acccatgttc
     1921 agaatcgacg ccagggctgc gctgaggcga tgcggtttgc accagacatg cggcaccggg
     1981 tcgagatgga gcacgatctg cgcctggttg aacgacggag gattcatcaa cgccacagcg
     2041 tcctggatca actgattgac atcggtaagg cgaatcgcgc tgcggtccag gttcgcgaat
     2101 ctctgaattc gcgctatagt ctcactcaaa cgcgcggccg attgctgaat agcgtctgac
     2161 agctcctcca ccagctccgg catatggctg cctttgggga acgaagcatg tctctcgacg
     2221 ccgcgaatca atgtgtctgt tgcactcttc agcgctccga tcggggtgtt caactcgtgg
     2281 ctcaaagagg cggccaagcg ttccattgta acggcgcttt cggccattaa cgcccgcgat
     2341 cgggccgctt ccgtctcctt ggcggattgc acggctgccg cgtgcgcatt gtagagtcga
     2401 atgcgttggg ttgtgaatct ggcggagatc gccaccgaga tagcgaccga caggagggct
     2461 gtggcggagt ccatgaagct tcctttgagt ggaaccccca tgaaagcagc cgatgcggca
     2521 caacttagca gcagcaacac gcccagtccc agcacacgaa gcgggcgaag cggcatcaat
     2581 gctgccccga gcatgagcag gatgaagatg gccgggatca cggaaggcgc aggcgcccca
     2641 tcttgagccg attgaagtaa gcgatccatg gttaccgctg caaccgaagt gccgagtagc
     2701 gctgccgtcg caactaaagc gagtgtggga gattgaatgg atctggaagc ccagaacccg
     2761 aagattccgg cgaccgcgag cacggcccac accgccagcg atgcggtgtg atgagaagcc
     2821 ggaggactat cccactgtag gattttcatc aggaacatcg cggccgcggt gatgcaaaca
     2881 agcaggctca ggactctgaa tccgtcgatt acttccgttg cctgctggcg ccggtatgcc
     2941 ggatcgggcg gatccgccgc aaccggcgcc agccaggaga gagccgcgtt cagcttgctt
     3001 gccacgaacg ttatggaagc aaatcgagtg ccaaggatgc ccgcgcgcgc cgggcgccgt
     3061 ggaagcaggc gtgcaactcg tcaaaggtgc ggacgatgcg agacaagcca tgcgcacact
     3121 tcttctgagc ttggtactta ccggtatcgt cagggtgccg gtggccggtg ccaatcggct
     3181 gccaacagcg gtgatcgtgg tgatggaggg cagtcccggg cagcagggcg aggccgcaag
     3241 taagattgtt ggcgcactga agacggtgct tggctgccgc cataccttgc aggtagcccg
     3301 gtttgacgcg gagccggggc tgcaatccag gctcaccgtg aaaggtgcga accgggacct
     3361 tatggcagtg gagccgcgca cgccgatgag agaagcgata gaagccggtc tcatccagct
     3421 gatcacagtt tcaagcccgc gcacaatggt cgtcatagct catgaacagt tctatcccac
     3481 caccgtttca aaagaccgtt tgctcgaatt ggcccggcgc tcggaagcaa agatccacac
     3541 aatttacctg gcatcgaacc gcgacaagcc cggagtgctc cggcgctttg gacggtcgtt
     3601 gcgaaatggg attgtgtggc tggtcgaggt aatcatcctg gaagaacggg gatactcggc
     3661 acgcgatact tcgcacctgc tgaagttcat ggcggatgcg accggaggga agtattgcgt
     3721 agccaccgac gaatcgagcg gaattgtctg tgcagacggc atctctgctg cgatcgccgc
     3781 tgaaccacaa ctctattcgc agccgtgaac atattgaacg gcaaagtcat cggcgcatgg
     3841 tagctcgtgg gccggtagcg cggcggagcg attgtttcca ggacgatcag cactggggcg
     3901 gcagatccga gacgctcggc ggagtccacg aagcctccct taagtggaac cccatgaagg
     3961 cggcggcctc ttcgagcgca gacactgaat cagatccgtc gtctgccggg agtggtcacg
     4021 gatggaagcc gccgcgcaga attccggtcc agtaggcgtc catgactccg acccacgtga
     4081 gcccgaaatg cgtcaatgac gcggcgaacg cattgccgcg tggcatctct gtctgccaac
     4141 gcggcgtctt cgatatcaga tcggctaccc tgcgcttcaa ctcttcgttt ttctgcaatt
     4201 gaccgggtgt aggaacggcc gagatcacgt gccggttctg agcgagcatg atgatctgca
     4261 gttccggacc gtcgctgcca taccgctcgg caattctcgc gccggtctgc agcgcctcca
     4321 accacgccgt cttcagcggc agttccgtct cgagttcaaa cagcggcacg agcgagtatt
     4381 gcgtctgcca gatggtcccg atatccgcgt gctccggacc gcgcgccaga tcgcgaggcg
     4441 tgtacgtgta gcgaagctga acagggtcga gcactgtgcg cagcctcgcg tagcgtcctt
     4501 cgcggagctt ctcaagatat acgtcacgcc agtgtggatc atccgtgatc gcgtgcccca
     4561 ccaagtacat ttgcaggagc ctggacgagc ggtccgagcg gatagcttcg atgtcgctca
     4621 cccagccggg cgctccattg ctgcccagaa tcgtcccgtc gcgctcaagc atcgtgcaga
     4681 tgtcctgcat gatgcggcgc atcgaagcct tttccggttc gcccgccaaa gcggagtgga
     4741 agtatttgta taaaccgtat acgtagaagg tgtattgatc gaccgacgag ttgtggaagt
     4801 gcgcgcgaga agcgtcaccg gggagcaacc cgcgtgcaat aaagccaggc cgcttgctgg
     4861 cggtggccag actctcaagc cctgagaaga cggcgcgcgc ctggcgaatg cactgcggat
     4921 cgccggtaac cgcgtggatg tccaccagtt gccccaggaa cacgccgccc gcgcctgcgc
     4981 agtttgagag attcggaact tcactatcgt tgtttggttt gccgcgcaca gcctccggcg
     5041 tcgcaaagcg aacgtgtttc cagagctgtg ggtcgtcgag attcacccgc gagtagatga
     5101 gttttgtctc agggtgaaca gcgcgctcat agaaccagtt cttcgcggcg atcgcttctt
     5161 cgatcgatgg caattgctgt gctacagccg gcagccatgc caggagcaac aatagtaccg
     5221 gtaacttcat ttgtgtttcc gtacgaagtc cacacactcg ttactgttgg aaaacgtgtg
     5281 cggcacgttg tagagccggt ggtcaccgtg aaggcactcg cgctcggtct tcggtggacc
     5341 gaagggtttg cgcaaaggtt cggtggcaa
//
LOCUS       DASTMK010000391         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_339246, whole genome shotgun sequence.
ACCESSION   DASTMK010000391 DASTMK010000000
VERSION     DASTMK010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339246"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFQ79_16970"
     CDS             <1..346
                     /locus_tag="VFQ79_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141418.1"
                     /translation="ATIVPLRYNSAMAKASKGMFEVSCPCCEATLKIDPETRAVISHQ
                     EKPKPPTFESMEAAFTRLKGEAGKREEAFQKSVQEHKSHADVLNRKFDELLKHARENP
                     DAPPPKRDIDFD"
     gene            complement(359..1108)
                     /gene="tpiA"
                     /locus_tag="VFQ79_16975"
     CDS             complement(359..1108)
                     /gene="tpiA"
                     /locus_tag="VFQ79_16975"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU0141419.1"
                     /translation="MRTPLMAGNWKMYKNAGETTQFFEKFKPLVEKSEHCEIAICPPF
                     VNIAAAVEAARGTRIGIGGQNVFWAKEGAYTGEVSAPMLKSLGCEWVIIGHSERRQYF
                     AETNETVLKRTLAALEAGLKPIVCVGETLEEREGGATESVLAEQFAGGIGPLDPAQFA
                     RIVIAYEPVWAIGTGRTATPEIASEAHRFIRSQVGTKFGADAAGAVRILYGGSVKPDN
                     VKKLMAQADIDGALVGGASLEPNSFASIVNF"
     gene            complement(1145..>1600)
                     /locus_tag="VFQ79_16980"
     CDS             complement(1145..>1600)
                     /locus_tag="VFQ79_16980"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688151.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU0141420.1"
                     /translation="GEIFLGRGICRTQKTKNNADGMEKTTAALHELREGLIFTNLVDF
                     DQLYGHRNDSEGYARALEAVDEWLPELRASCRDEDLVIFTADHGCDPTTPSTDHSREY
                     VPVLAWGPKAAAGRALGTRETLSDIGQTVAENFGVSIANGSSFLDEIVA"
BASE COUNT          310 a          498 c          465 g          327 t
ORIGIN      
        1 cgcaacgatt gttccgctgc gctacaattc cgctatggcg aaagcgagca aagggatgtt
       61 cgaagtgagt tgcccgtgtt gtgaggccac gctgaagatc gaccctgaga cacgcgcggt
      121 aatcagccac caggagaagc cgaaaccgcc cacgtttgaa agcatggaag ccgccttcac
      181 ccgtctgaag ggagaagccg gcaagcggga agaggcattc cagaagtcgg ttcaggagca
      241 caagagccac gcggatgtgc tcaacagaaa gttcgacgaa ctgctgaagc acgcgcgaga
      301 gaatccggac gcgcctcctc ccaagcgcga tatcgacttc gactgacagg accggacgtc
      361 agaagttcac gatcgaagcg aacgaattcg gttcgaggct cgctccgcct accagcgcgc
      421 cgtcgatgtc ggcttgcgcc atcagtttct tcacgttatc cggcttgacg ctgccgccgt
      481 aaagaattcg gacagcaccc gcggcatccg cgccgaactt ggttcctacc tgggaacgga
      541 taaaacggtg cgcttcggag gcgatctccg gcgttgccgt ccgcccggta ccaatggccc
      601 acaccggctc gtaggcgatg acaatccggg caaactgcgc ggggtcgagg ggtccgatgc
      661 cgccggcgaa ctgttcggcg agcacacttt ccgtcgcgcc gccctcgcgc tcctcgagtg
      721 tttctccgac acatacgatc ggctttaacc ccgcttccaa tgcagccagc gttctcttga
      781 gcacggtctc gttcgtctcg gcgaagtact gcctgcgctc gctgtggccg atgatcaccc
      841 actcgcagcc aagggacttg agcatgggag cggaaacctc gccggtatag gcgccttcct
      901 tggcccaaaa tacgttctgg ccgccaatgc cgatgcgcgt tccccgggct gcttcgacag
      961 cggccgcgat gttgacaaaa ggcggacaga tcgcgatttc acaatgctcg gatttttcca
     1021 ccagcggctt gaatttctcg aaaaattgag ttgtttcgcc ggcgttcttg tacattttcc
     1081 aattgccggc catcagcggt gttctcatag gtatctcaga taagtgggtc cttaatattg
     1141 tagcttacgc tacgatctca tccagaaaac tgctgccgtt cgcgatagag acgccgaagt
     1201 tctccgccac cgtctggccg atgtcggaaa gcgtttcgcg cgtgccgagc gcccgccccg
     1261 ccgccgcttt cggcccccag gcgagcactg gcacgtactc gcggctgtga tcggtggagg
     1321 gagttgtggg gtcgcagccg tgatccgcgg tgaagatgac cagatcctcg tcccggcacg
     1381 acgcccgtag ctccggcagc cattcgtcga ccgcctccag cgcgcgtgcg tagccctcgc
     1441 tatcgttccg gtgcccgtag agctgatcga agtccaccag gttggtgaag atcaatcctt
     1501 ccctaagctc gtggagcgcg gctgtggttt tctccatgcc atccgcgttg ttcttcgtct
     1561 tttgggtgcg gcagatgccg cgtcccagaa agatctctcc
//
LOCUS       DASTMK010000392        10613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_403661, whole genome shotgun sequence.
ACCESSION   DASTMK010000392 DASTMK010000000
VERSION     DASTMK010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403661"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFQ79_16985"
     CDS             <1..854
                     /locus_tag="VFQ79_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141421.1"
                     /translation="QGDRSEPSSITAEEAARAEGQGQQKGNEGTEGETEGEQADGAMP
                     ASDESGAQEKQAGNSGDAPRDKQASSQSGNQQGNQEDSGLMQKMREAMANLLSKLKIQ
                     PPPGAGQQSAQAGKSGQERQKQASSGKGQPAPGKQQGEGEQADSSQGEQDEGSEATRS
                     ARGNQGDRNSDQRGSEDSKSGVGKTDGDKDLKAAEQLAAMGKLSEIIGKRAANVTGEV
                     MVEVASGDQQLRTPYSRRHATHAQAGGEIHRDEVPLVYQHYVQQYFEEIRKAPLPEPA
                     DSANPAK"
     gene            885..2462
                     /locus_tag="VFQ79_16990"
     CDS             885..2462
                     /locus_tag="VFQ79_16990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEU0141422.1"
                     /translation="MSRLSELQRSTFDVVVTGGGIVGSGIARDAALRGLKVALLEKND
                     YGSGTTAGSTRLVHGGLRYLEMLDFRLVRMDLREREILLRIAPHLVRPLRFVLPFYDR
                     RLALRLKLRTGMTLYDALSFDRSLPGHRYIPKEEVLRGEPSLSTCGLQGAVAYSDAQV
                     VSPERLCLENILDARENGCIALNYAEVTGALREHGRVCGVRTRDVLTGETVEVSGRVV
                     INASGPWLDAVAQSLQPTGRIRIRTTKGIHIACSPVSKQAYVLTSPLDGRLFFVIPWL
                     GYSWIGTTDTDFTGDPASVRATAEDVAYLVESASAFFPTLRDARIFWTNAGVRALVMR
                     KGRPSDISRLHRIVDETPNGSPGLISVLGGKITGYRAIAEEATGAACALLSRRRKSAT
                     AALPLPGARDDAPSGSLPHLQAIYGSRAAEVAALADAGESLARPLDAAYPDITAQVIH
                     AVRSEQAVRVGDFLLRRSYLGFSEDRGMRALAAVADTMAAESGWSAARKSAEIEEYTR
                     WTAQTQEFRNPAAAHRR"
     gene            2539..3024
                     /locus_tag="VFQ79_16995"
     CDS             2539..3024
                     /locus_tag="VFQ79_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU0141423.1"
                     /translation="MKTYGKFAVLIVAIIGTLVWLAAGGIQEGKTYYKTVSELRDMKD
                     AEGKRLRVAGDVEAGSIIRKGREVEFTLVQESQKLKVVYGGIDPLPDTFRDGAQALAD
                     GKLGADGVFRAAKIQAKCASKYEAKPGGNQGAPVYQKKAGGSTPAGSYPADTPQRQAG
                     L"
     gene            3049..5058
                     /locus_tag="VFQ79_17000"
     CDS             3049..5058
                     /locus_tag="VFQ79_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEU0141424.1"
                     /translation="MENLGALAILLAFCLSLYSVVGSLIGKWKKNLFLTISAQRAVYS
                     IWFLLTAASGLLVYALMTGDFRMAYVAAHSNHAMPALYKFAAWWGGQEGSLLFWSWLL
                     ATYSAIVVFTNRRKFREMMPYVVSILMATQCFFLILNAFVVSPFQVLAVGRGITEVPD
                     GNGLNPLLQYWSMAIHPPMLYLGYVGFIVPFAFAMGSLITRQPGDSWIHTTRRWTLVT
                     WLFQSCGILLGAGWAYAVLGWGGYWAWDPVENASLLPWITATAFLHSVMMQEKKGMMK
                     VWNMVLISATFFLCIFGTFLTRSGVVQSVHAFAQSPIGKYFVVYLTVGIAATAYLILD
                     RLDYLKSESHLESVVSREASFLFNNLILLASCFAVLWGTMFPVISEAVTGEQVSVDAP
                     FYNRINVPIGLFLIFLTGVGPLIAWRRSSIESLKRAFMWPTIGGLALMVALFAAGVRH
                     FYALMSFGLCLFVAWTIVSEFWKGANAIRAKSGGNLFGAMFELTHRNTRRYGGYVVHM
                     GMVFMFIGFTGAAFNKNLITETELGGVLHIGKYELKVSDLRDGENQNYAWNHAVVEVR
                     SGGKFLGALEPERRFYKSSGQSTSEVAIRRRVNEDLYLNYAGVNENQKPVIQAYIFPL
                     VSWIWIGFATLIFGTAICLVPSKVRLQYARTEVVGMVGKHAPAEK"
     gene            5039..5506
                     /locus_tag="VFQ79_17005"
     CDS             5039..5506
                     /locus_tag="VFQ79_17005"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEU0141425.1"
                     /translation="MLRLRSSFLLLALAALSLAQTGMTLATPEIRRVGDKLACLCGSC
                     KNSVGDCPMIMCHYSNPAREKIASMQKAGFSDQAIIDEFVKKEGIRALVAPPAEGFSL
                     VGWLMPFVAIGIGLVMIWMFLRRFRKPAAVPEIDPGDFDRYRDRIDKDLAKLD"
     gene            5506..5931
                     /locus_tag="VFQ79_17010"
     CDS             5506..5931
                     /locus_tag="VFQ79_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141426.1"
                     /translation="MLLISAGILTVGAILYTLFVRAKDLPEPVPPSPVAHLEERKARI
                     YEGLRDLQFEYRVGKLSDADYQQTKLDLQKELAAVMAEIDRILGVKPAPKPAVAPQPT
                     AAPPERPGVYVCPHCGAEFPQALKFCGECGKPMTGEAGA"
     gene            5928..6926
                     /locus_tag="VFQ79_17015"
     CDS             5928..6926
                     /locus_tag="VFQ79_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141427.1"
                     /translation="MRLPALFLLASLAFAAVDGTVMNRTSGKPEANATVTLYRLDQTG
                     MQPVKTVRAAADGKFQFDAKVEGPHLLQAIHRGVAYNHMLSPGSPSTGIELAVFDSSS
                     KPGIAPVAQHMVLLEPSEGRLAVSETFFLRNQSNLTYNNSEQGTLRIYLPEEAVESAR
                     VMATAPNSMPVERPSQKTNTPGIYLIDFPIKPGETRIDLTYQLPFSGTGKFSGRVLEK
                     EGTARLVVPSGVTIQGEGLEMLGIEPSTQAQIYGLKTGAYAVEITGTGALRATDRGED
                     EAPGIQQILPRIYDRLYWVLGLSLAILGIGFYLLYRSPNKEVQASAPPARTGRKAK"
     gene            6923..7624
                     /gene="ccmA"
                     /locus_tag="VFQ79_17020"
     CDS             6923..7624
                     /gene="ccmA"
                     /locus_tag="VFQ79_17020"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEU0141428.1"
                     /translation="MSAVRVEGVWKYYGHFPALRNVSFQVEPGACLALLGRNGAGKTT
                     LLKIIAGLSRPAGGQVLIADKEARDRETRGRIGILGHGIAVYDELSAFENLKLFGRLY
                     GLRDPARTALEWLERTGLERVRDGLVREFSRGMRQRLAVARAFLHSPSILILDEPFTA
                     LDDRAIAVLQGLLEKALSEGRTVIMSTHQLREAMQLATDVALLNRGKLAYHGERTAEM
                     LADPGWLYRHYGEVA"
     gene            7621..8301
                     /locus_tag="VFQ79_17025"
     CDS             7621..8301
                     /locus_tag="VFQ79_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU0141429.1"
                     /translation="MIFLRQSLTIAVKDLRAELRSKEAINASLSFAIVILLLFSFAFD
                     PSSEQTREISGGLLWLVFAFAGALILNRSFARELTNDCLEALIASPVSGAALFLGKAL
                     SNFVVLIAIELLSLPVFGIFYNVRWTGQFWTLMMILLLATWGLTVIGTVFSALTVNLR
                     LRELMLPTLVYPILIPALLPAIQLTSAVIAGQPLTADLYAGLRLLIAFDVIFTALALG
                     LVETILVG"
     gene            8305..8988
                     /gene="ccsA"
                     /locus_tag="VFQ79_17030"
     CDS             8305..8988
                     /gene="ccsA"
                     /locus_tag="VFQ79_17030"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU0141430.1"
                     /translation="MRRNIMIGLCVIAAALLARNLNVILLHLPDEANQGAIYRIFYFH
                     LPAAMTAGACAFAALVSSILYLATKNFRYDSIAVSVTEVGLAFGAMVLATGMIWARII
                     WGIWWTWDARLTSMLIVWLMYAGYLMLRRAIDEPTARARNAAVLSIFIAPGVYITWKS
                     IEWWRTQHPGPVLSIRGGGGMAPGMESAAYWNLLALLLIAVVLVMVRMQQETYQRRLD
                     ALRREAHAF"
     gene            9210..9368
                     /locus_tag="VFQ79_17035"
     CDS             9210..9368
                     /locus_tag="VFQ79_17035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HEU0141431.1"
                     /translation="MDERNFTYMFYGFLAAWLILVAYVVLLVLREKKIEREMESLKRM
                     VEDRGRKA"
     gene            9365..>10613
                     /locus_tag="VFQ79_17040"
     CDS             9365..>10613
                     /locus_tag="VFQ79_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0141432.1"
                     /translation="MKRRSLLRMATAGAASGLLTKREAAAQAQVQRATSGMPSPKIKD
                     ITAIETAPAGLRLTVVKVTTDQDGLYGYGCATFTQRADLVRPAVERYLKPLLVGKPAD
                     RIEDTWQMCYNSSYWRNGPVLNNAISGVDQALWDIKGRQAGMPVYQLLGGKAREAADC
                     YGHASGGEIAEVIDSARKWMDRGFRHVRIQVGVPGMAGYGSGRGAAKVEALHSAPIFE
                     PAAYIRRALALFEQARKELGDEIELLHDVHERISQSQALQFCKDVEKFRLFFLEDPVS
                     PEDIAWFRLMRQQCTTPLAMGELFNSPHEWNPLIAERLIDYIRVHVSQAGGLTPCRKM
                     AAMGEIFGVKTAWHGPGDVSPVGHAANVTLDVACYNFGIQEYSTFNERTQEVFQGCPV
                     MKNGYLYANEAPGWGIEVNEKLAA"
BASE COUNT         2070 a         3113 c         3306 g         2124 t
ORIGIN      
        1 agcagggcga ccgctcagaa ccgtcttcga taaccgccga agaagctgcg agagcggaag
       61 gccaggggca acagaaaggc aacgagggaa ccgaaggaga gacggagggc gaacaggcag
      121 acggcgcaat gccggcgtct gacgaatccg gcgcgcagga aaaacaagcc ggcaactccg
      181 gcgacgctcc acgcgacaaa caggcttcgt cccagtccgg caatcagcag ggcaaccagg
      241 aagattccgg tttgatgcag aagatgcggg aggccatggc gaacctcctc tccaagctca
      301 aaattcagcc gccgcccggc gccggccaac aatccgcgca ggccggcaaa agcggccagg
      361 aacggcagaa acaagcttcg tccggaaagg gccaacccgc gccgggcaag cagcagggcg
      421 aaggcgaaca ggcggattcg tcacagggcg aacaggatga aggctccgag gcaacccgct
      481 ccgcgcgggg gaatcagggt gaccgtaata gtgatcagcg cggttccgag gactcaaaaa
      541 gcggcgtcgg caagaccgac ggcgataagg acctgaaggc cgcggagcag ttggccgcga
      601 tgggtaagct gagtgagatc atcggcaagc gcgccgctaa cgtaactggt gaagtcatgg
      661 tggaggtcgc ttccggcgat cagcaactga gaacccccta ctcacgccgt cacgccacac
      721 acgcgcaggc cggcggggaa attcaccggg acgaggtccc gctcgtctac cagcattacg
      781 tgcagcaata tttcgaagag atccggaagg cgccgctgcc cgaaccggct gattccgcaa
      841 atcccgccaa gtaaccctcc tgggtccgtg cctgggcgaa agcgatgagc cgcctcagcg
      901 aactccagcg ttccactttt gacgtggttg ttaccggggg cggcatcgtc ggctccggca
      961 ttgcacgtga cgcggctttg cgcggcttga aggttgcgct tctcgaaaag aacgattacg
     1021 gatcgggaac gacagcgggc tctacacgcc tcgtacacgg cggcttgcgt tatctcgaga
     1081 tgctcgattt tcgcctggtg cgcatggacc ttcgcgaacg cgagatcctt ctccgcatcg
     1141 ctcctcacct ggtccggcct ctgcgttttg tgcttccgtt ctatgaccga aggctcgcat
     1201 tgcgcctgaa gctccgcacg ggaatgacgc tctacgacgc attgagcttt gaccgcagcc
     1261 ttccgggaca tcggtatata ccgaaggagg aggttctccg cggcgaacca tcgctctcca
     1321 cgtgcgggtt gcagggcgcg gttgcgtatt cggatgcgca ggtagtatcg cccgaacggc
     1381 tatgtctcga aaacatcctg gatgcgcgcg agaacggatg catcgcgctc aactatgcgg
     1441 aggtaacggg cgcgcttcgc gaacacggca gggtctgcgg cgtgaggact cgggatgtac
     1501 tcacgggaga aacagtggag gtaagcggcc gggtagttat caacgcgtcg ggcccgtggc
     1561 tggatgcggt cgcgcaatcg ctccagccca ccggtcgcat ccgcatccga accacgaagg
     1621 gtatccatat cgcgtgctcg ccggtttcaa aacaggccta cgtcctcacc tcaccgctgg
     1681 atggccggtt attcttcgtc attccatggc tgggctacag ttggatcggc accaccgaca
     1741 ccgacttcac cggagacccg gcatccgtgc gcgctacagc ggaggatgtg gcttacctgg
     1801 tggagtcggc atcggctttc ttccccacct tacgggatgc gcggattttt tggaccaacg
     1861 ccggggttcg cgcgctggtc atgcggaaag ggcggccgtc cgacatctcc cggctccacc
     1921 gcatcgtaga cgagacgccg aacggttcgc ccggactgat ttccgtcctt ggaggtaaga
     1981 ttacgggcta tcgcgccatc gccgaagaag ccaccggcgc ggcttgcgcg cttctgagcc
     2041 gccgcaggaa atcggcgacg gcggcattgc cgttgccagg cgcgcgcgac gatgcgccct
     2101 ccggctcgct gcctcatctt caggcgattt acgggtcccg cgccgccgag gtcgccgcac
     2161 tggcggatgc cggcgagagc ctcgcgcggc cgctcgacgc cgcctacccg gacatcaccg
     2221 cgcaggttat tcatgccgtc cgctccgaac aggcggttcg tgtaggcgat tttcttttac
     2281 gaagaagcta tcttggcttc agcgaagatc ggggaatgcg cgccctggca gcggtggcgg
     2341 ataccatggc ggcggaatca ggatggagtg ccgcgaggaa atcggcggaa atcgaagagt
     2401 acacacgatg gaccgcgcag acacaggagt tccggaaccc ggccgccgca cacaggcgtt
     2461 aaatcgaagc gatgggcttc gatgggcgcg gaaaatcggc aaaataaagc tatactattg
     2521 ggttaagagg cagcacttat gaaaacttac ggtaagtttg ccgtcctcat cgtggccatc
     2581 atcgggacac tggtctggct ggcggctggc ggaattcagg aaggcaaaac ctactataaa
     2641 acggtatccg agcttcgcga catgaaggat gccgagggca aacggcttcg tgtggccgga
     2701 gacgtagaag cgggttctat tattcgaaag ggccgcgaag tcgaattcac tctggtgcag
     2761 gaatcgcaga agctgaaggt tgtctatggc ggcatcgatc cgctgcccga cacgttccgc
     2821 gacggcgcac aggcactggc ggatggcaaa cttggcgccg acggcgtgtt tcgcgcggcc
     2881 aagattcaag cgaagtgcgc ttccaagtat gaggccaaac ccggtggaaa ccagggtgcg
     2941 cctgtgtatc agaaaaaagc cggcggatcc acacccgcgg gctcttatcc ggcggatact
     3001 ccacaacgtc aagccggact gtaatcggga attcgtcttc ggtgtgaaat ggaaaatctg
     3061 ggcgctctcg ctattctgct cgccttctgc ctctccctct actcggtagt tggatcgctg
     3121 attggaaaat ggaagaaaaa cctctttctg acaattagcg cgcagcgggc cgtttattcc
     3181 atctggtttc tgctgactgc tgcttcgggt ctgctggtct atgcgctcat gacgggcgac
     3241 ttccggatgg cgtatgtagc cgcccactcg aaccacgcca tgcccgcgct gtacaaattc
     3301 gcggcatggt ggggaggcca ggaaggttct cttctgttct ggagctggct gctggccacc
     3361 tactcggcca tcgtcgtgtt caccaaccgg cgcaagttcc gggagatgat gccgtacgtg
     3421 gtttccatcc tgatggccac gcagtgcttc ttcctgatcc tcaacgcctt cgttgtgagc
     3481 ccgttccagg ttctggcggt gggccggggc atcactgaag taccggacgg caacggtctc
     3541 aaccctctgc ttcaatactg gagcatggcg attcacccgc ccatgctcta cctgggctac
     3601 gtcggcttca tcgttccgtt cgctttcgcc atgggatcgc tgatcacacg ccagcccggc
     3661 gactcctgga ttcacaccac gcgccgctgg acgctggtga catggctgtt ccagagctgc
     3721 ggcattcttc tcggtgcggg ttgggcgtat gcggtgcttg gatggggcgg ctattgggca
     3781 tgggacccgg tggaaaatgc ctccctgctg ccctggatta ccgcgacggc atttctgcat
     3841 tccgtgatga tgcaggagaa gaagggaatg atgaaggtct ggaacatggt cctgatttcc
     3901 gccaccttct tcctctgtat attcgggacg ttcctcacgc gctccggcgt cgtgcaatcc
     3961 gttcatgcgt tcgcgcaatc gcccatcggc aagtacttcg tggtgtacct gacggttggc
     4021 atcgccgcaa cggcctatct gattctggac cgcctggatt acctcaagag cgaatcgcac
     4081 ctcgagagcg tagtttcgcg cgaagccagc ttcctgttca ataacctgat tctgctggca
     4141 agctgttttg cggtgctgtg gggcacgatg ttcccggtga tttcggaagc cgtaacgggc
     4201 gagcaggtga gcgtggatgc tccgttctac aaccgcatca atgtgccgat cggcctgttc
     4261 ctgatttttc tgacgggcgt aggcccgctg atcgcgtggc ggcgcagttc gattgaaagc
     4321 ctgaagcgcg cttttatgtg gcccaccatc ggcggccttg cgctcatggt tgccctgttt
     4381 gccgcgggtg tgcggcactt ctacgctctc atgtctttcg gcctgtgcct gtttgtggca
     4441 tggaccatcg tctccgaatt ctggaagggc gccaatgcga ttcgcgcaaa gagcggcggg
     4501 aatcttttcg gagccatgtt cgagctgacg caccgcaaca cgcggcgtta tggcggatac
     4561 gtagtgcaca tgggcatggt gtttatgttc atcggcttca ccggcgccgc attcaacaag
     4621 aacctcatca cggaaaccga gttggggggc gtcctgcaca tcggaaaata cgaattgaag
     4681 gtgtcggatt tacgtgacgg ggagaaccag aattacgcct ggaatcacgc cgtcgtcgag
     4741 gtccggagcg gcgggaaatt tctgggtgcg ctcgagccgg agcgccggtt ttacaagtca
     4801 agcggccagt ccacctccga ggtagccata cggcggcggg tgaacgaaga cctgtatctc
     4861 aactacgccg gcgtgaacga gaaccagaag cctgtcattc aagcctatat tttcccgctg
     4921 gtttcctgga tctggatcgg ctttgcgacg ctgatcttcg gtacggccat ctgcctggtt
     4981 ccgagcaagg tccggttgca gtacgcgcgc accgaagtgg ttggtatggt aggaaagcat
     5041 gctccggctg agaagtagtt ttttgctgct ggcgctggcc gcgctttctc tcgcgcagac
     5101 cggcatgacg ctggccacgc cggagattcg ccgtgtcggc gacaagctgg catgcctttg
     5161 cggcagttgc aaaaattccg taggtgactg ccccatgatc atgtgccact attcgaatcc
     5221 ggcgcgggag aagattgcca gtatgcagaa ggccgggttc tccgatcagg cgatcatcga
     5281 tgagtttgtg aagaaggaag gaattcgcgc cctcgttgcg ccgccggcgg aaggcttcag
     5341 cctggtcgga tggctgatgc cgttcgtcgc catcggaatc gggcttgtca tgatctggat
     5401 gttccttcgc cggttccgga aacccgctgc ggttccggag atcgatcccg gcgatttcga
     5461 ccgctatcgt gaccgcatag ataaggacct cgcgaaactc gactaatgct cctgattagc
     5521 gccggcatcc tgactgtagg ggctattctc tatactctct tcgtccgcgc gaaggacttg
     5581 ccggaacccg tcccgccgtc tccggtagcg cacctggaag agcgaaaagc ccgcatctac
     5641 gaaggcttgc gggaccttca attcgaatat cgtgtcggca agctctccga cgcggactat
     5701 caacagacca agctcgacct gcaaaaagag ctggccgcgg tgatggcgga aatcgaccgg
     5761 attctgggcg taaagcccgc accaaaaccg gcggtggctc cccagcccac cgcggcgcca
     5821 cccgaacggc ccggcgtcta cgtctgcccg cattgtggcg ccgagttccc acaggcgttg
     5881 aagttctgcg gcgagtgcgg aaaaccgatg acgggagagg ccggcgcatg aggctccccg
     5941 ctttgttcct gcttgcgtcc ctcgccttcg ccgccgttga tggcaccgtg atgaaccgca
     6001 cgtccggcaa accggaagca aatgccacgg tcacgctgta ccgtctcgat caaacgggca
     6061 tgcagcccgt gaagaccgtg cgcgccgcgg cggacggcaa attccagttc gacgcgaagg
     6121 tggaaggccc gcacctgctt caggcgatac accggggcgt ggcgtacaat cacatgctct
     6181 cgcccggcag cccgtccacc ggtatcgagc tcgctgtttt cgactcgtcc tcaaagccgg
     6241 gtatcgcacc agtggcgcag cacatggtgc tgctggagcc gtcggaaggg cggctggcgg
     6301 taagtgaaac cttcttcctt cgcaaccaga gcaatctaac ctacaacaat tccgaacagg
     6361 gaacactccg gatttatctt cctgaggaag cggtagagtc cgcgcgcgtg atggcgacgg
     6421 cgcccaatag catgccggtg gaaaggcctt cgcagaagac caacacacct ggtatttatc
     6481 tgatcgactt ccccatcaaa cccggtgaaa ccagaatcga tctaacctat cagctcccat
     6541 tcagtgggac aggaaaattc tcaggcaggg ttttggagaa ggaaggaacc gcgcgactgg
     6601 tggttccctc gggtgtgacc attcaaggcg aggggttgga gatgctcggc attgagccca
     6661 gcacgcaagc tcagatttat gggttgaaga cgggcgccta tgcggtggag atcaccggga
     6721 ccggggctct gcgcgctacg gatcgtgggg aagacgaagc tcccgggatc cagcagattc
     6781 tcccacgcat ctacgaccgc ctgtactggg tgctcggact ttcgctcgcc attctcggca
     6841 tcggtttcta cctgctctac agaagtccca acaaagaagt ccaagcttcc gccccgccgg
     6901 cgcgaaccgg acgcaaggcg aaatgagcgc cgtccgcgtg gaaggcgtat ggaagtacta
     6961 cggccacttc cccgccctgc gcaatgtcag cttccaggtt gagccgggcg cgtgcctcgc
     7021 actgctgggc agaaatggcg cgggcaagac gacgctcctg aaaattatcg ccggcctttc
     7081 ccgtcccgcg ggcggccagg tgctgatcgc cgacaaggaa gcgcgggacc gcgaaacgcg
     7141 tgggcgtatc ggcattctgg gccacggcat tgcggtttac gatgagctct cggcgttcga
     7201 gaatctcaag ttgttcggcc ggttatacgg tttgcgcgac cctgcgcgga ccgcgctcga
     7261 atggctggag cgcacgggcc tcgaacgggt gcgcgacggg ctggtacgcg agttctcgcg
     7321 cggtatgagg cagcggctgg ccgttgcacg ggccttcctg cactccccgt ccatcctgat
     7381 tctggacgag cccttcaccg ctctcgacga ccgggcgatt gctgttcttc agggcctgct
     7441 cgaaaaggca ctgtcggaag ggcgcaccgt catcatgtct acgcaccagc ttcgcgaagc
     7501 gatgcagctc gcaaccgatg tcgctctgct caaccgtggc aaactcgctt accatggcga
     7561 gcggacggcg gagatgcttg ccgaccccgg ctggttatac cggcactacg gagaggtggc
     7621 gtgatcttcc tgcggcaatc ccttaccatc gccgtcaaag accttcgcgc ggagttaaga
     7681 agcaaggagg ccatcaatgc atcgctctcc tttgccatcg tgatcctgtt gctgttcagc
     7741 ttcgctttcg acccgagctc ggagcagacg cgcgagatct ccggagggct gttatggctg
     7801 gtgttcgcgt tcgccggcgc cctgatattg aatcgcagct tcgcccgcga gttgacgaac
     7861 gattgcctgg aggcgctgat cgcctccccc gtttccggcg cggcgctgtt tctgggcaag
     7921 gcgttgtcga acttcgtcgt gctgattgcg atcgagttgc tttctcttcc ggtgttcggc
     7981 attttctata atgtccgctg gaccgggcaa ttctggacat tgatgatgat tcttctgctg
     8041 gcgacgtggg gcctgaccgt aatcggcacc gtgttcagcg cgttgaccgt gaacctgcgg
     8101 ctgcgcgagc tgatgttgcc tacgctggtg tacccgattc tgataccggc cctgctgccg
     8161 gctattcagt tgaccagcgc ggtgatcgcc gggcagccgc tcacggcaga cctgtacgcc
     8221 gggctgcggc tgttgatcgc gttcgacgtc attttcacgg cactcgcgct aggcctggtg
     8281 gaaacgattc ttgtggggtg aggtatgcga cgaaacatca tgatcggcct ttgtgtcatc
     8341 gccgcggctc ttctggcacg caacctgaac gtgattctgc tgcacctacc ggatgaggcg
     8401 aaccaggggg ctatctaccg gatcttctac tttcatctgc ctgcggcgat gaccgcgggc
     8461 gcctgcgctt tcgcggcgct ggtgtcgagc attctatatc tggcgacgaa aaatttccgc
     8521 tacgattcga tcgccgtctc tgtcaccgaa gtggggctcg ccttcggcgc gatggtgctg
     8581 gcgacgggga tgatctgggc gcgcatcatc tggggaatct ggtggacctg ggacgcccgg
     8641 ctgacatcga tgctgattgt ctggctgatg tacgccggtt acctgatgct gagacgcgcg
     8701 atagacgagc cgacggcgag agcgcgcaac gccgcggtgc tgtcgatatt catcgcaccc
     8761 ggcgtctata tcacctggaa gtccattgaa tggtggcgca cgcagcaccc cgggcccgtc
     8821 cttagcatac gcggcggcgg cggaatggct ccgggcatgg agtcggcggc ttactggaac
     8881 ctgctcgcgc tgcttctgat cgcggttgta ctggtgatgg tcagaatgca gcaggaaacc
     8941 taccagcgcc ggctcgatgc tttgcggcgt gaggcacacg cgttttgaaa cacgacgtcc
     9001 taaatatttg cgcgacgatt tgattggcca cggatggaca cagatgaaca cagataagaa
     9061 caatgcaagg cttgatccgt gtgaatccgt gttcatccgt ggccgaattt gcttttttag
     9121 attgctgaga tgcgcaatta tttatggcgt tctgttttag attttccaca ttcctgctgc
     9181 gatagggcga tactggagaa cgaatcgtta tggacgagcg caatttcact tacatgttct
     9241 acggctttct cgcggcctgg ctgattctgg ttgcctacgt cgtgctgctg gtcctgcggg
     9301 aaaagaagat tgagcgcgag atggaaagcc tgaaaagaat ggtggaagac cgggggcgca
     9361 aggcatgaaa agacgcagcc ttctccggat ggccactgcc ggcgccgcat cgggtctcct
     9421 gacaaagcgt gaggcggcgg cccaggcaca ggtgcaacgc gccaccagcg gcatgccatc
     9481 accgaagatc aaggacatta ccgccatcga aaccgcgccc gcgggcctgc ggcttacagt
     9541 cgtcaaggtg acgacggacc aggacgggtt gtacggctac ggttgcgcta ctttcaccca
     9601 gcgcgcggac ctggtgcgcc cggccgtcga acgatacctc aagccgctcc tggtgggcaa
     9661 gcctgccgac cgcatcgaag acacctggca gatgtgctac aacagctcct actggcgcaa
     9721 cgggccggtg ctcaacaatg cgatcagcgg agtagaccag gcgctgtggg acatcaaggg
     9781 ccgccaggcc ggtatgccgg tgtatcaatt gctgggcggc aaggcgcggg aagccgccga
     9841 ttgctacggc catgccagcg gcggcgagat cgcggaagtc atcgatagcg ccaggaagtg
     9901 gatggaccgc ggcttccgcc acgtacggat acaggtcggc gtgccgggca tggctggtta
     9961 cggttccggg cgcggcgcgg caaaggtaga ggcgctgcat tccgctccga tttttgaacc
    10021 cgctgcttac atccgccgcg cgctggcact gttcgaacag gctcggaaag aactcggcga
    10081 cgagatcgaa ttgctgcatg acgttcacga gcgcatcagc cagagccagg ccctgcaatt
    10141 ctgcaaggac gtggagaagt tccggctgtt cttcctggaa gacccggtct cgccggaaga
    10201 catcgcgtgg ttccggctca tgcggcagca gtgcactaca ccgctcgcga tgggcgagtt
    10261 gttcaacagc ccgcacgaat ggaatccgct gatcgctgaa aggctcattg actacatccg
    10321 ggtgcacgtc tcgcaggcgg gcggcctgac gccctgccgg aaaatggcgg cgatggggga
    10381 aatcttcggg gttaaaaccg cctggcacgg tcccggcgac gtttctcccg tcggacatgc
    10441 cgccaacgtg acgctcgatg ttgcctgcta caacttcggc atccaggaat attccacgtt
    10501 caacgagcgc acgcaggaag tgtttcaggg ctgccccgtc atgaagaatg gctacctgta
    10561 cgcgaacgaa gctccgggtt ggggcattga agtaaacgaa aagctggcgg cga
//
LOCUS       DASTMK010000393         5701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_532501, whole genome shotgun sequence.
ACCESSION   DASTMK010000393 DASTMK010000000
VERSION     DASTMK010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532501"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFQ79_17045"
     CDS             complement(<1..364)
                     /locus_tag="VFQ79_17045"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU0141433.1"
                     /translation="MEDLETSAPEKAEETESVGADSERQPEETAAGDLVPEQLLIGDD
                     SSAAPDPDTELRAVLEAIVYITDEPLSSQQIAFALGQPQEKIEALLEELAERYARPEH
                     GLMFRQVAGGYKITTKPEH"
     gene            complement(371..1162)
                     /locus_tag="VFQ79_17050"
     CDS             complement(371..1162)
                     /locus_tag="VFQ79_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU0141434.1"
                     /translation="MIEVPSPLDIRLEHYEGPLDLLLDLIRKQQINIYDIPIASITAQ
                     YLDYVQKAMELDVELGSEFIYMAATLIHIKSRTLLPRDPELDKIAPEEDPRKELVDRL
                     IEHERFKNAAEMLQQKRVIEEAVWSNPQIDQFIAEDENPGLSVTLFDLVKTLQTVLER
                     ARTRPVYEVSKEDVSVPAMIAYLRELMTLRPTQTLRAIELFESQHSRRAMICLFLAIL
                     ELVRRQALVLEQGDAFGDIEIARHENFDSVFASGEAIEAVEKEYS"
     gene            complement(1202..1996)
                     /locus_tag="VFQ79_17055"
     CDS             complement(1202..1996)
                     /locus_tag="VFQ79_17055"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0141435.1"
                     /translation="MRRWVPLAAAGILTSLFLVAQNSPDVTDGEMHGDAPYLIEEGWK
                     PLLNGKDLSGWHAQGNKPSEWFTTRGVRWERLLGPTRLSAAPSPGGTILNGPNGRTAN
                     LVTDEKFGDVELYLEFMISKGSNSGVYLHGLYEVQVFDSYGSIEPMKTSDGGAIYHRW
                     INNQGVGGSAPARNASRRPGEWQSYYIWFRGPRFNGGKKTEDARFVRVLHNGVLVQDN
                     VICEGPTRAHMDLPEAAENPLMLQGDHGPVAFRNIYIRPLRPIVVR"
     gene            complement(2015..2920)
                     /locus_tag="VFQ79_17060"
     CDS             complement(2015..2920)
                     /locus_tag="VFQ79_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0141436.1"
                     /translation="MARSPRSSLRDRAEYAAAYLLLSSLRLGSRRFAGMLARGYVAVL
                     DRAVPKLRRTAIANLERAYPEWPPAQRKAVADDVFHSIARLLVAFARFPSIDRSNVSE
                     WIRYEGREHFEEAKRQGRGVLFATAHLGNWELSAFAHGLMAEPMNVVVRPLDNPLIDR
                     LVEHRRTLSGNRIIEKKEAARAILKALDRNEAVGILIDQNSGLDQGVFVDFFGIPACA
                     SVGLARLAAHSGALVIPGFALWSEKENKYVLRFHAPIEMSGDAARDTARLQAMLEGVI
                     REHPSQWLWIHRRWKTRPPGEPPLY"
     gene            complement(2970..4076)
                     /gene="mnmA"
                     /locus_tag="VFQ79_17065"
     CDS             complement(2970..4076)
                     /gene="mnmA"
                     /locus_tag="VFQ79_17065"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU0141437.1"
                     /translation="MADPPVAVAMSGGVDSSTVAALLRKQGRQVIGLTMQLWNQRRLP
                     GLSAEGTHGRCCSLDDVYDARRVAQQLGIPYYVLNFEQRFEEQVVRPFIQEYLDGRTP
                     VPCTLCNNFVKFDDFIDFAAGLGVERIATGHYARIAWNEETGRHELRRGADASKDQTY
                     FLFGLTQRQLSRTLFPLGDLTKEEVRKLAAELEVPVAKKPDSQEICFVPNGDYAAFID
                     AYFEEQGIPANAASGEIVDTSGKVIGGHSGVHHFTVGQRRGLGIAAGEPLYVIETQPA
                     SQRVVVGRNQDLLRRELVARDVNWISIPDLTEAVRAEVKIRNKHIAAPATLLPTGDAK
                     RTRVLFDEPQRAITPGQAAVFYQGDLVAGGGWIE"
     gene            complement(4069..5265)
                     /locus_tag="VFQ79_17070"
     CDS             complement(4069..5265)
                     /locus_tag="VFQ79_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEU0141438.1"
                     /translation="MAVRIEVIAVVNRFYFDHNATTPVSSEVLAEHIEALREVYGNAS
                     SIHHNGQQAKQALEEARRRVAAAIGADPREIVFLSGGTEADNLAIRGVVRASTNPRKH
                     VITSAIEHPAVMNTCAQLEREGIEVTYVPVASSGVIDPDDVRRALREDTVLVSLMHAN
                     NETGTLQPVEAIGRITREAGVVFHSDGVQAFGKIPVDVNTLGVDLYTISGHKIYAPKG
                     VGALYVRKGTKLAPLLFGGHHERDRRPGTENVPGAVALGCAAAAAVRDLEAESSRLDA
                     LRNRLERAILEQVPHSGVNGSAAARVPNTTNIYFDGVQGEAMVIALDLTGFAVSTGSA
                     CSSGAVEPSHVLTALGLTKERARASLRFSLGRSNTAAQVDALVEAVVEAARRLRSLSP
                     AYVHHG"
     gene            5264..>5701
                     /locus_tag="VFQ79_17075"
     CDS             5264..>5701
                     /locus_tag="VFQ79_17075"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEU0141439.1"
                     /translation="MSARIANLPAFLLIAFSQAATAQLTSWSTQSQAAVGPRNQAIAQ
                     RIASDHQGNTYVAANLLQDVAFPSALSNVPELRYDVALIKLDPTGKEVFRAELRGSGN
                     DTVAGLAVDSAGNAYLTGFTNSTDFPVFRPYQREHRGESGSAFV"
BASE COUNT         1071 a         1803 c         1697 g         1130 t
ORIGIN      
        1 ggtgctcggg tttcgtggtg attttgtacc cacccgccac ctggcggaac atcaggccgt
       61 gctccggacg cgcataccgc tcggcaagct cttcgaggag agcctcgatc ttctcctgcg
      121 gctgtccgag agcgaacgcg atctgctgcg aagatagtgg ctcgtcggtg atgtagacga
      181 tggcctccag gacggcacgc agttcggtat cggggtccgg ggcggcgctt gaatcgtcgc
      241 caatcagcaa ttgttccggc accaggtccc cggctgccgt ttcttccggt tgccgctcgc
      301 tatccgcccc cacggattcc gtctcttctg ccttttcagg ggcgcttgtt tcgagatctt
      361 ccatcgttgg ctagctgtat tccttctcaa ctgcttcgat cgcttcaccc gacgcgaaga
      421 ccgaatcgaa attttcgtgg cgcgcgatct cgatgtcgcc gaacgcatcg ccctgttcga
      481 gcaccagcgc ctggcgtctc accagttcca ggatggccag gaacaagcag atcatggcgc
      541 gccgggagtg ctggctctca aaaagctcga tggcacgcag cgtctgggtg gggcggagcg
      601 tcatcaattc ccgcaaatag gcaatcatcg cgggcacgct gacatcttcc ttgctgacct
      661 cgtagaccgg cctggtcctg gcgcgctcca acaccgtctg gagagtcttg acgaggtcaa
      721 acaaggtcac cgaaagcccg ggattttcat cttcggcgat gaactgatcg atctggggat
      781 tcgaccacac cgcctcttcg atgaccctct tctgctggag catctccgcc gcattcttga
      841 accgctcatg ttcgatgagc cggtctacca gttctttccg ggggtcctct tcgggcgcga
      901 tcttgtcgag ttccggatcc cttggaagaa gcgtcctgga tttgatgtgg atgagggtcg
      961 ccgccatgta gatgaattcg gaaccaagct ccacgtccag ctccatcgcc ttctgaacgt
     1021 agtcgagata ctgcgccgtg atggaagcga tggggatgtc gtagatattg atctgctgct
     1081 tgcggatgag gtcgagcagc aggtcgagcg gaccctcgta gtgttccaga cgtatgtcca
     1141 gcggtgacgg cacttcaatc acgataacat aagcttccag gcgccctgcc ggcgccgtgg
     1201 atcaccggac aacgatgggc cggagcggcc ggatgtagat gttgcgaaag gctacggggc
     1261 catgatcgcc ctgtagcatc agcggattct cagcggcttc ggggaggtcc atatgcgcgc
     1321 gcgtggggcc ttcgcaaatg acgttgtcct gtaccagtac gccgttgtga agcacgcgga
     1381 cgaagcgcgc atcctccgtc tttttgccgc cattgaaacg aggcccccgg aaccagatgt
     1441 aatacgactg ccactcgccg gggcgcctgc ttgcattgcg cgccggcgcg gagcctccga
     1501 cgccctgatt gttgatccaa cggtgataaa tggccccgcc atcggaggtc ttcatcggtt
     1561 cgatagagcc gtagctgtcg aagacctgca cctcgtagag gccgtgcagg tagacgccgg
     1621 aattcgagcc tttcgaaatc ataaattcga gatagagctc gacatcgccg aacttctcgt
     1681 ccgtcaccag gttggcggta cgcccgttcg gaccgttgag gatcgtccct ccgggggatg
     1741 gtgccgcgga cagcctggtg gggcccagga gccgttccca gcgtacgccc cgcgtggtaa
     1801 accattcgct cggtttattg ccctgcgcgt gccatccact caggtctttg ccgttgagga
     1861 gcggcttcca gccttcctcg atcagataag gggcatcccc gtgcatctcg ccgtccgtta
     1921 catcgggaga attctgcgcc acgaggaata gtgaggttag aatgccagcc gccgcaaggg
     1981 ggacccaccg tctcatgagt gacagcgtac caccttagta aagcggcggc tcgcccggcg
     2041 ggcgcgtctt ccagcggcga tggatccata gccactggct ggggtgctca cgaatcacgc
     2101 cttcgagcat cgcttgcaga cgcgccgtat cgcgcgccgc gtccccgctc atctcgatcg
     2161 gcgcatggaa ccgcagcacg tacttgtttt ccttttccga ccagagagca aagccgggaa
     2221 tcactagggc cccgctgtgc gcggccagtc tcgccaatcc aaccgacgcg cacgcgggaa
     2281 tgccgaagaa gtcgacgaag acgccttgat ccaggcccga attctgatcg atgaggatgc
     2341 ctaccgcttc atttctatcg agggctttca ggatggcgcg ggcggcttct ttcttctcga
     2401 tgatgcgatt gccggagagg gtacgccgat gctccaccag gcggtcgatg agcggattat
     2461 ccagcggcct cacgaccacg ttcattggtt cggccatcag cccgtgcgca aacgcgctga
     2521 gctcccagtt gcccaggtgc gcggtggcga agagaactcc cctgccctgc cgcttggcct
     2581 cttcgaaatg ttcgcgccct tcatagcgaa tccattcgga gacgttggac ctgtcgatgg
     2641 atggaaagcg cgcgaacgcc accagcaggc gcgcgatgga atggaatacg tcatcggcga
     2701 ccgctttgcg ctgcgcgggc ggccactccg gatacgcgcg ttcgaggttt gctatcgcgg
     2761 ttcgccgcag cttcggcacc gcgcggtcga gcacggcaac ataaccacgg gccagcatgc
     2821 cggcaaagcg gcgcgagcca agacgcagcg acgacagcag cagatacgcg gcggcgtatt
     2881 ccgcgcgatc acgcaaagag gaacgagggg accgggccat tgatcaatga atcaatgtat
     2941 caatgaactc cctggcggat tcatcggcgc tattcgatcc agccgcctcc ggctacgaga
     3001 tcgccctggt aaaagacggc cgcctggcct ggcgtaatcg ccctttgcgg ttcgtcaaac
     3061 agcacgcggg tgcgcttcgc atcgccggtg ggcaggagcg tcgcaggagc ggcgatgtgc
     3121 ttgttgcgaa ttttgacttc ggcccggacg gcttcggtaa ggtccggaat cgagatccag
     3181 ttcacatcgc gcgccaccag ctcgcggcgg aggagatcct ggtttcgccc caccactacg
     3241 cgctggctcg ccggttgggt ctcgatgacg tacagcggct cgcccgcggc aataccgagc
     3301 ccccgcctct ggccgacagt gaaatggtga acgcccgaat gcccgcctat aacttttccg
     3361 ctggtgtcga cgatctcgcc ggatgcggcg tttgccggaa tgccctgctc ctcgaaatac
     3421 gcatcgatga aagcggcgta gtctccgttc ggcacaaagc agatttcctg gctgtccggc
     3481 ttttttgcta ccggcacttc gagctcagcc gccagtttgc gcacttcctc cttggtcagg
     3541 tccccgagcg gaaatagggt gcggctcaac tgccgctgcg tcagaccgaa caggaaatag
     3601 gtctggtctt tcgaggcgtc cgcgccgcgg cgcagttcat gacggcccgt ctcttcgttc
     3661 caggcgatgc gcgcgtaatg gccggttgcg atgcgctcca cgcccagccc tgcggcaaaa
     3721 tcgatgaagt cgtcgaattt tacgaagtta ttgcacaacg tgcacgggac cggtgtacgc
     3781 ccgtccagat attcctgaat gaacggccgg accacctgct cttcgaatcg ctgctcgaag
     3841 ttcagaacgt agtaaggaat tccgagttgc tgtgcaacgc gccgcgcatc gtacacatcg
     3901 tcgagcgaac agcagcggcc gtgcgtgccc tccgccgaaa gtccggggag ccggcgctga
     3961 ttccaaagct gcatcgtaag gccgatgacc tgcctgccct gcttccggag cagcgcggca
     4021 acggtggagc tgtcgacacc accgctcatc gcaaccgcga ccggtggatc agccatggtg
     4081 gacataggcg ggtgacaggc tgcgcaaacg ccgggccgct tcgacgacgg cttcgaccag
     4141 cgcatccacc tgcgcggcgg tattcgagcg gcccagcgaa aagcgcaggc ttgcgcgcgc
     4201 cctctccttc gtcagcccga gcgcggtcaa aacatgggac ggttccacgg cgccgctcga
     4261 gcaggcggaa cccgtcgaga ccgcaaaacc ggtgaggtcc aaggcgatca ccatcgcctc
     4321 tccctgcacg ccatcgaaat aaatgttggt ggtgttgggc acgcgggcag ccgcgcttcc
     4381 attcacaccg gaatgcggaa cctgctccag gatggcgcgc tccaggcgat tgcgcagggc
     4441 atcgagccgc gacgactccg cttccagatc gcgaacggcg gccgccgcgg cacaaccgag
     4501 cgccaccgca cccggaacat tctccgttcc gggacgccgg tccctctcgt gatgtcctcc
     4561 aaataaaagc ggggccagtt tcgtaccctt gcggacgtac aacgcgccca cgcctttcgg
     4621 cgcataaatc ttgtgaccgc tgatggtgta gaggtccacc cccagcgtat tcacgtcgac
     4681 aggtatcttt ccgaaagcct gcacaccatc ggaatgaaac acgacgcccg cctcacgggt
     4741 aatgcgccca atcgcctcga cgggctgaag ggtcccggtt tcgttgttgg catgcatgag
     4801 cgagacgagc acggtatctt cgcgcaacgc cctgcgcaca tcgtcaggat cgataactcc
     4861 gctcgaagca accggaacat acgtgacctc gatgccttcc cgctcgagtt gcgcacaggt
     4921 attcatcacc gcggggtgtt cgatggcgct ggtgatgaca tgcttgcggg ggttcgtgct
     4981 cgcacgcacg actccccgga tggccagatt atcggcttcg gttccgccgc tcaggaacac
     5041 gatttcgcgc gggtccgcgc caattgccgc cgccactcgc cggcgcgctt cttcgagcgc
     5101 ctgcttcgcc tgctggccgt tatggtggat actggaggcg ttgccataca cctcgcgaag
     5161 cgcttcgatg tgctccgcga gcacctccga agacaccgga gtagtagcat tgtggtcgaa
     5221 ataaaagcgg ttcacaacag cgattacttc aattctaaca gccatgtccg ctcgcatcgc
     5281 aaacctgccg gcgttcctgc tgatcgcgtt ttcacaggcc gctaccgcac aactcacttc
     5341 ctggagcacg caatcccagg ctgcagtggg tccccgcaat caggcgatcg cacagagaat
     5401 tgcctcagac caccagggaa atacttatgt cgcagcgaat ctgcttcagg acgttgcctt
     5461 tccatccgcc ttatcgaacg tgccagagtt gcgctacgac gtggcactca tcaaattgga
     5521 ccccacgggt aaagaagtat tccgggctga actgcggggc agcggtaatg acacggtcgc
     5581 cggtcttgca gtggacagcg ccggcaacgc ctacctgacc ggcttcacca attccaccga
     5641 ttttccggtg ttccggccgt atcagcggga gcaccgcggt gaatccggca gcgccttcgt
     5701 c
//
LOCUS       DASTMK010000394         6741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_276497, whole genome shotgun sequence.
ACCESSION   DASTMK010000394 DASTMK010000000
VERSION     DASTMK010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276497"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFQ79_17080"
     CDS             <1..991
                     /locus_tag="VFQ79_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0141440.1"
                     /translation="TLLVAGCSALTQSDIKRVLAYSTVSQIGYMFLALGVGAWSAALF
                     HFMTHAFFKALLFLAAGIVIEALHHEHNIFRMGGLRRELPVAFWTFLAGGCTLAGLPL
                     ITSGYFSKDLIVWEAWSSPLGGLLLWTAGMAGVLLTALYTFRLIFLVFFGTRRTEVSH
                     RPGFRMKAPVIALAALAVVSGYWKNPLLDFLHTALPSMEEAHGGMTETMSGIIAATLF
                     AAGLAIAYLFFLARPQYSDAAARNPVFGMLHRYWFSGWGFDWLYDHVFVRPFLWTARI
                     NRSDFVDAFYTGIARLTEGANHLLVATQTGRVRWYAAGIAAGAVIFVGVAILL"
     gene            988..2472
                     /gene="nuoM"
                     /locus_tag="VFQ79_17085"
     CDS             988..2472
                     /gene="nuoM"
                     /locus_tag="VFQ79_17085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU0141441.1"
                     /translation="MILVWFIAILLAGGILAALAARASAAAARWIAFAAIAADFALGL
                     LLWTGAGRTSLAPDGRWIEQVNWAWIPQFGIHFHLALDGLSLLLLMLTFFLGILAVLA
                     SWTEITESVGFFHLNLLWVLAGVVSVFLAMDLFLFYFAWELMLVPMYFLIAMWGHERR
                     VYAAIKFFLFTQLSGLLMLVGILALYFLHYASTGVYTFQYDQLLGTALPPGTEFWIML
                     GFFIAFAVKLPVFPLHTWLPDAHTEAPTAGSIVLAGLLLKTGGYGILRFVVPLFPDAA
                     REFTPVAMTLGVVGILYGAILAFAQTDLKRLVAYTSVSHLGFVLVGIFAWNELALQGA
                     VMTMLCHGISTGALFVLAGALQERLHTRELGRMGGLWATIPRLSGAGLFFALASLGLP
                     GLGDFVGEFLVLFGAFPSHPLITIFATIGVLFATFYALRLVQRAFHGENVHGLSVRDL
                     VPREWAVVALMATSLLWLGLYPQPVLDTFSPAVNHLQTVAGIGR"
     gene            2477..3910
                     /locus_tag="VFQ79_17090"
     CDS             2477..3910
                     /locus_tag="VFQ79_17090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU0141442.1"
                     /translation="MSQAQAIAILPLLILTATPIVLMVGVAIRRSHTATAVLSLAGLA
                     AALASLRTAAAAAPMQVTPLLMIDAYSLFYTGLILSGTAAVVLLSWGYFEGRTRRPEE
                     FYILVLVAALGSVVLASANHFISLFLGLELLSVSLYAMIAYLHWRDLPVEAGIKYLIL
                     AAASAAFLLFGMALIYTELGTMEFGAMRFDAATNMHPAILLPALALIIVGIGFKLAVV
                     PFHMWTPDVYEGAPAPAAAFIATVSKGAMFALLLRFFYGSGAYGYEPVAVVFAMIAIV
                     SMIAGNVLALLQDNVKRILAYSSIAHLGYLLVAFLAAGDLAPAAITFYLVAYFITMLG
                     AFGVVGALSGPHADAGGLAEYRGLYWRSPVIAALFTAMLLSLAGIPLTAGFVGKFYVV
                     AAGTSRALWALVFVLVVTSAIGLYYYLRIVVALYSRGPETAAAGRPRLAPATGVALAI
                     LTFLLVAFGIFPQPLLEMIRNATAGLG"
     gene            complement(3921..4790)
                     /locus_tag="VFQ79_17095"
     CDS             complement(3921..4790)
                     /locus_tag="VFQ79_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141443.1"
                     /translation="MITLVTGFLVTGLVAMQVGNPKPADEQSSTAAGAQPIYRVSVVQ
                     RTIKAVNYGHRSRPTKVDLTGTVLVPAARGEAEVESRRGAVQIRVKVKDLPAPSTFGR
                     EYLTYVLWAITTEGRPANLGELVLDPGNEGKLQVSSELQSFALLVTAEPYFAVTQPSD
                     VVVMENVIRPDTVGRIEEVNARYELLPRGHYTYVQPSSGPDPSSEREKVSMDQYEAIA
                     ALYQALNAIQIARAAGADSAAADTMNKALELYEEARQLQSSNAGSRKVVMTARQAAQT
                     AEDARAITQKRSQ"
     gene            4922..5224
                     /locus_tag="VFQ79_17100"
     CDS             4922..5224
                     /locus_tag="VFQ79_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141444.1"
                     /translation="MDYEPETPFDDIEGAQQYLDLLGEAVIDARQEIEGEIALAKEMG
                     APRREQALMLISYNLAKLGSHMTKSRRILNDLRTLRRLLLEERSMKAAASKNGKLA"
     gene            complement(5235..5957)
                     /locus_tag="VFQ79_17105"
     CDS             complement(5235..5957)
                     /locus_tag="VFQ79_17105"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU0141445.1"
                     /translation="MMMSIRGPMLSLLLLCGTVYAQSNVTQATIPSITASGDAVIQAQ
                     PDRARIDLGVVSQASTAQEASAQNAKQVEQVLAGLRKALPDTKNIRTRGYSLHPNYHH
                     PRDGGQPTITGYTASNIVEITVDDVKTVGEIVDSAIKAGANNVNGLEFMLRNEQEFRS
                     RALREAATKARANAESLAAALGLKVARVLHVQEGSPAVIRPVREMAMQRMAADAGSAV
                     PMEPGTIEVRAQVTLTVEVRGQ"
     gene            complement(6003..>6741)
                     /locus_tag="VFQ79_17110"
     CDS             complement(6003..>6741)
                     /locus_tag="VFQ79_17110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU0141446.1"
                     /translation="ILLKAGVEVPPAESARKAPESESVPVFHPAPEVNWVPSPEPTPR
                     RPFRKWIVAGASAVLLLLVLSASWWLGERHEEAVVKPRASNPQPPVRNTETPATRPSP
                     MVDDSAAAVQQPPPFVASSGERSKKAGSPTRDADLSLADATSSADRQADGVGTADYSG
                     TELDGNRTASGEPFDSKSLTAAHPDFPIGTQVRVTNLRNDRSVVVRINDRTALPQGHV
                     IRLTIGAARELGFAGAGRARVRVERVE"
BASE COUNT         1105 a         2030 c         2038 g         1568 t
ORIGIN      
        1 aacgctgctg gtggccggat gcagcgcgtt gacgcaatcg gatatcaagc gcgtgctggc
       61 atactcgacg gtgagccaga tcgggtacat gttcctggct ctgggtgtag gagcgtggtc
      121 cgccgccctg tttcacttca tgacgcacgc cttcttcaag gctcttctct ttctggccgc
      181 gggcatcgtc attgaggcgc tgcatcacga acacaatatc ttccgcatgg gaggccttcg
      241 gcgggaactg ccagtcgcgt tctggacttt tcttgcaggc ggctgcacgc ttgcaggcct
      301 gccgctgatc acatccggct acttcagtaa agacctgatt gtttgggaag cctggagttc
      361 cccgcttggc ggtctgctgc tgtggaccgc gggcatggcg ggtgtgctgc taaccgcact
      421 ctacacgttc cggttgatct tcctcgtatt tttcggcaca cgcaggacgg aggtcagcca
      481 tcgtcccgga tttcgcatga aggctccagt cattgcgctc gccgcgctcg cggtggtgag
      541 cgggtactgg aaaaatccac tgctcgattt cctgcacacg gcgctgccat cgatggaaga
      601 agcccatggg ggcatgaccg agacgatgtc aggcatcatc gcggcgaccc tgtttgccgc
      661 gggcctggct atcgcgtacc tgttcttcct cgcgaggcca caatattccg atgccgccgc
      721 gcggaacccg gtattcggga tgctgcaccg gtactggttt tccggatggg gtttcgactg
      781 gttgtacgac cacgtcttcg tgcgtccgtt cctttggacc gcgcgtatca atcggagtga
      841 tttcgtcgat gcgttttata cgggaatcgc gaggcttacg gaaggggcga accatctgct
      901 ggtggcgacg caaacggggc gagtccgctg gtatgcggcc ggaatcgcgg ctggtgccgt
      961 gattttcgta ggggtggcga tactcttatg attcttgtct ggttcatcgc gattcttctg
     1021 gcgggaggca ttctggcggc gctggccgcg cgcgccagcg ccgccgccgc acggtggata
     1081 gcgttcgctg ccattgctgc cgacttcgct ctcgggttgc tgctctggac cggcgccgga
     1141 cgcaccagcc tggcgccgga tggcaggtgg atcgaacagg tgaattgggc atggattccg
     1201 cagttcggca tccacttcca tctcgccctc gacggtctca gcctgttgct gctgatgctc
     1261 accttctttc tgggcattct cgcggtgctc gcctcgtgga cggagattac cgaaagcgta
     1321 ggcttcttcc atctgaacct gctgtgggtg ctggcggggg tagtcagcgt cttcctcgcg
     1381 atggacctgt ttcttttcta ttttgcgtgg gagttgatgc tggttcccat gtacttcctc
     1441 attgcgatgt ggggacatga acgccgcgtc tatgccgcca tcaagttttt cctcttcacg
     1501 cagctcagcg gactcctgat gctggttggg attctcgcac tctatttcct gcactacgcc
     1561 agcaccggag tatacacgtt ccaatacgat caacttctcg gcacggcgct gccgccgggc
     1621 acggaattct ggatcatgct gggcttcttc attgccttcg cggtgaaatt gcccgtgttc
     1681 ccgctgcaca cctggctccc ggacgcgcac accgaggctc ccacggccgg cagcatcgtc
     1741 ctcgccggac tactgctgaa aaccggcgga tacggcatct tgcgttttgt cgttccgctg
     1801 tttcccgatg ccgctcgcga gttcactccc gtcgctatga ctctgggtgt cgtgggaatc
     1861 ctttacggtg cgatcctggc ttttgcgcag acggatctga agcggctcgt cgcctatacc
     1921 agcgtcagcc atctgggctt cgtgctggtg ggaatctttg cctggaacga gctcgctctt
     1981 cagggtgccg tcatgaccat gctgtgtcac ggcatcagca cgggcgctct gtttgtactt
     2041 gccggcgctt tgcaggaacg tctgcacacg cgtgaattgg gccgcatggg cggtctttgg
     2101 gcaaccattc cccggctcag tggcgcgggt ctcttttttg cgctggcctc tctcgggttg
     2161 ccgggattag gggatttcgt cggcgagttt ctcgtgctgt tcggagcatt cccttcgcac
     2221 cctctcatca cgatcttcgc gaccatcggc gtcctgttcg ccactttcta cgcactgcgg
     2281 ctggtccagc gcgcattcca cggcgagaac gttcacggcc tgagcgttcg cgacctggtt
     2341 ccacgggagt gggccgttgt cgcactaatg gccaccagtt tattgtggct cgggctgtat
     2401 ccgcagccgg tgctcgacac gttcagccct gccgtaaatc atttgcaaac ggttgcggga
     2461 attgggagat gaccttgtga gccaagcaca agcaatcgcc attttgccgc tgctgatttt
     2521 gacggcgact cccatcgtat tgatggtagg cgtagccatc cggcgcagcc acaccgccac
     2581 ggccgtcctg agtcttgccg gactcgccgc cgcgttagca tccctccgga ccgcggccgc
     2641 cgccgcgccc atgcaggtta cgccgctgct catgatcgat gcctactcgc tgttctacac
     2701 gggcctgatt ctaagcggta ccgccgcggt ggtattactt tcgtggggct atttcgaggg
     2761 ccgcacaaga cgcccggagg agttctacat cctagttctg gttgcggcgc tggggtcggt
     2821 ggtactggca tcggccaatc acttcatttc gctattcctc ggcctggaac tgctgagcgt
     2881 ttcgctgtac gcgatgatcg cctatctcca ctggcgcgac ttgccggtgg aagccggaat
     2941 aaaatatctg atcctcgcgg ctgcgtccgc cgcgttcctg ctgttcggaa tggcgctgat
     3001 ctacacagaa ctgggcacga tggagttcgg cgcaatgcgg ttcgatgccg cgacgaatat
     3061 gcatcctgcg atcctgttgc ctgcgctggc gctcatcatt gtggggatcg gtttcaagct
     3121 tgctgtagtc ccatttcata tgtggacgcc ggatgtgtac gaaggcgccc cggcaccggc
     3181 tgcggcattt atcgcaacgg tgtcgaaagg ggccatgttt gcgcttctgc tacggttctt
     3241 ctacggctcg ggcgcgtacg gatacgaacc cgtagcggtc gtctttgcga tgatcgcgat
     3301 cgtttcaatg attgcgggca atgttctggc gctgctccag gacaacgtga aacgaatcct
     3361 ggcctactca tccatcgctc atctcggata cctgctggtg gcatttctgg cggccggaga
     3421 ccttgcgccg gcggccatca cgttttacct ggtggcatac ttcattacca tgctgggcgc
     3481 tttcggcgtt gtgggcgcgc tttccggacc ccacgcggat gccggcggcc tggcggaata
     3541 ccgcggcttg tactggcgca gcccggtgat cgcggcgctc ttcaccgcga tgcttctctc
     3601 cctggccgga attccgctga cggccggctt tgtaggcaag ttctatgtgg tagcggcggg
     3661 tacatcgagg gcgttatggg cgctggtgtt cgtgctggtc gtcaccagcg cgatcgggct
     3721 ctactactat ctgaggatcg tagttgccct gtacagccgc ggtccggaaa ccgcagcggc
     3781 ggggcggccg cgtctggcgc cggcaaccgg ggtcgcgttg gcgattctca ctttcctgct
     3841 ggttgccttc ggaatatttc cgcagccgtt gctcgagatg attcgcaacg cgactgccgg
     3901 tctcggatag tgaaagcgct ctattgactt cgcttctggg tgattgcccg tgcatcctcg
     3961 gccgtctgcg ccgcctgccg ggccgtcatc acgactttcc tgcttccggc attgctcgat
     4021 tgcaactgcc tcgcctcctc gtaaagctcc aatgccttgt tcatggtgtc cgccgcggcg
     4081 gaatcggcac cggctgccct cgcgatctgg atggcgttca gggcctgata aagcgctgcg
     4141 atggcttcat actggtccat cgaaaccttc tcacgctcgc tcgaagggtc cggcccggac
     4201 gacggctgca cgtaggtgta atggcctcgc ggcagcaatt cgtacctggc attcacctct
     4261 tcgattctgc ccaccgtgtc gggccggatc acgttttcca taacgaccac gtcgctcggt
     4321 tgggtgacgg cgaagtacgg ctccgcggtg acgagtaaag cgaaggactg aagctcagaa
     4381 gagacctgga gcttgccctc attgcccggg tcgagaacca gctctccgag attcgccggc
     4441 ctaccctcag tggtaatcgc ccacagcaca taagtaaggt attccctgcc gaatgtcgat
     4501 ggcgccggca gatccttcac ttttacccgg atctgtaccg ctccacgcct gctttcgact
     4561 tcggcttcgc cgcgggctgc gggcacaagc acagtgcctg tcagatccac cttggtgggc
     4621 cggctgcggt gcccatagtt aaccgccttg atggtgcgct gaactacgct gacacggtaa
     4681 atgggttgag cgccggccgc cgtgctgctt tgctcgtccg caggtttagg atttccaacc
     4741 tgcattgcca ccaggccggt taccagaaat ccagtgacaa gtgtaatcat aagcccctct
     4801 ataacgagta ttgcaggtag gcgcggcggg tgcagcgcgg cttcggcggc atcgtgttgc
     4861 agatttcggg cgaaacagtt gcaaaacggt ttataattcg taggtccggc caggaggtgg
     4921 agtggattac gaaccggaga ccccatttga cgatatcgaa ggcgcacagc agtaccttga
     4981 ccttcttggt gaggccgtga tcgatgccag gcaggaaatt gaaggtgaga ttgcgctggc
     5041 caaggaaatg ggtgcaccgc gccgcgaaca ggcactcatg ctcatctctt ataaccttgc
     5101 gaagctcgga tcgcatatga cgaaaagccg ccggattctg aacgatttga ggacgcttcg
     5161 ccggctgctg ctggaagaga gaagcatgaa agccgcggcg tcgaaaaacg gcaagctagc
     5221 ctgacaattg tttgttattg gccgcggact tcgacggtaa gtgtaacctg agcacgtact
     5281 tctatcgtgc ccggttccat cgggaccgca ctcccggcat cggctgccat cctctgcatc
     5341 gccatttcgc gcaccggacg gatcacggcc ggtgatcctt cctgcacgtg cagcacccga
     5401 gccactttca agcccagggc ggccgcgagc gattccgcgt tggcgcgcgc cttggtagcc
     5461 gcctctctga gcgcccggga gcggaattcc tgctcgttcc tgagcataaa ctccaatcca
     5521 ttcacgttat ttgcaccggc tttgattgcc gaatccacga tctccccgac agtcttcacg
     5581 tcgtccaccg tgatctcgac gatattcgaa gccgtataac cggtgatggt tggctgtccg
     5641 ccgtcacgcg gatgatggta gttcgggtgg agcgaataac cacgcgtgcg aatattcttc
     5701 gtgtcaggca acgctttccg gagcccggcc agcacctgtt ccacctgctt cgcgttttga
     5761 gccgatgcct cctgcgcggt agaggcctgg ctgaccacgc ccaggtcgat tctggcgcga
     5821 tccggctgcg cttgaatgac ggcatcgcca cttgcggtga tcgatggtat cgtcgcctgt
     5881 gtgacattcg attgggcgta aaccgtcccg cagagcagca gcagcgaaag cataggaccg
     5941 cgaatggaca tcatcattct cctctttcag gatatgcctt tgaagcggct gtcctttcgc
     6001 cgctactcca ctctttctac tctgacgcgg gcgcggcccg ctccggcgaa accgagctcg
     6061 cgggcggcgc cgatggtgag gcggatgacg tggccctgcg gcagtgcggt gcggtcattg
     6121 atccggacga ccacgctacg gtcgtttcgc aggttggtga cgcgcacctg tgttccgatt
     6181 ggaaagtccg gatgagccgc ggtcaagctc ttgctgtcga agggttcacc gctcgcagtg
     6241 cggtttccat caagctccgt gccggaataa tccgccgtac cgactccatc tgcctggcga
     6301 tcggcgctcg acgtggcatc cgcgagagac aaatccgcgt cgcgcgtagg ggaacctgct
     6361 ttcttggacc tttctccact ggacgcgacg aacggcggcg gttgctgcac tgctgccgcg
     6421 ctgtcatcaa ccatcggact gggccgggtt gccggagttt ccgtattgcg caccggcggt
     6481 tggggattgc ttgccctcgg tttgacaaca gcttcctcat gccgttcgcc taaccaccag
     6541 gaggcagaaa gaaccagcag cagcagaacg gcacttgctc cggcaacgat ccacttacgg
     6601 aacggccggc gcggcgtggg ttctggagag ggcacccaat tgacttcggg tgccgggtga
     6661 aacacaggaa cgctttcgga ctcgggcgct tttcgagcgg attcggcggg cgggacctca
     6721 accccggcct tcagcaggat g
//
LOCUS       DASTMK010000395         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_179852, whole genome shotgun sequence.
ACCESSION   DASTMK010000395 DASTMK010000000
VERSION     DASTMK010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179852"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFQ79_17115"
     CDS             <1..873
                     /locus_tag="VFQ79_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU0141447.1"
                     /translation="IALIGFRNAGIIVTSETTAVAMGNLRDPHTLLSIFGLLLISGLL
                     VWGVRAAMLVGVLATTALGAITGLVQWRPQAYSFSDISATAFQLDIAGAIRLGLLEII
                     FVFLFVDLFDNVGTLVAVGKKARLFDEAHQIPRINRILYSDATATMAGSLTGTSTVVS
                     YIESAAGVVAGGRTGVPAVVAGLLFLVALFIAPAMGVVPAAATAPALILVGSFMLTHV
                     AEIQWDDPVVAIPAFLTITTIPLSFSIANGLSFGFTAYALLRLARGEFRKVNWLVWLL
                     AALFIVRFAYLGGG"
     gene            903..1994
                     /locus_tag="VFQ79_17120"
     CDS             903..1994
                     /locus_tag="VFQ79_17120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0141448.1"
                     /translation="MHGHGNAGGPRPRLVFWELTTGCNLRCIHCRASATELMSPDDLN
                     YQESCDVIDQIAEYAPLILVLSGGEPLWRRDVFDLAHRARSKNIRVALATNGTLVDEA
                     MAQRIQDAGIQRVAISLDGADRATHDNFRGHSGAFDAAIRGLRCVQDLGISTQINTTV
                     SHHNVHQLPDIMKLAETLGVNAFHIFLLVPVGCGLTISEDQSVKGREAEQILEWFYER
                     SLESTMELKATCAPQYYRIVRQKRAEAKRAGLSVPDLPTHGMNAVTRGCLAGQGVCFI
                     SHRGEVYPCGYLPLAAGDLRRQRFRDIWERATVFATLRDSGNLDGKCGFCEFRQVCMG
                     CRARAYGVYGNFLAEEPFCEYQPRTGVHA"
     gene            2114..2611
                     /locus_tag="VFQ79_17125"
     CDS             2114..2611
                     /locus_tag="VFQ79_17125"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU0141449.1"
                     /translation="MPVVTKPETPAVKDNRERFLERAMSRHHYSGDALIEVLHTAQEL
                     YGFLTPALLKTIARKLRLPPSRVLGVATFYHFFSLEPKGEHSMIVCTGTACYVAGAQG
                     LMRILEHRCAKAGGTSPDGKMSVQAARCIGSCGLAPAVIYDGKILPRVTPDQLNAELD
                     RIGVR"
     gene            2608..4284
                     /locus_tag="VFQ79_17130"
     CDS             2608..4284
                     /locus_tag="VFQ79_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NuoF family protein"
                     /protein_id="HEU0141450.1"
                     /translation="MTFEDLLELQAKEIARQQGLRERIYCCTAAGCISSGADAVKKAI
                     DEEIKARGVGADVECCGTGCMGLCSKGPLVRAQSDGAIYAAITPEDAGALVAGEDANL
                     LAGRKLDPQADFFTRQEKIVLANSGTVDPEKLSDYIAAGGYQALARAVMELTPQQVIE
                     EVKRSGLRGRGGAGYSTGLKWELVAKQPVDTKYVVCNADEGDPGAFMDRSILEGDPHR
                     ILEGMAIAGYAVGAQQGYIYVRGEYGLAIQRLQAAIKQAERERLLGSRIFENSFQFRI
                     DLRIGAGAFVCGEETALIASIEGKRGQPRQRPPYPPESGLWGKPTLINNVETFGNIAP
                     IVAKGSEWFATIGTSASKGTKVFALAGKIENTGLIEVPMGTSLRTIIFDIGGGIPGGK
                     EFKAAQTGGPSGGCIPPQFLDVPVDYESLHKIGSIMGSGGLIVMDDTSCMVDVARFFM
                     DFCMDESCGKCIPCRAGTVQMHDILQRITKGEANDNDLVVLEQLAKLLRETSLCGLGQ
                     TAPNPVMSTLNYFSGEYQAHIKDHRCPAGQCTLDGEEAAATVNGNGKVQA"
     gene            4281..>4810
                     /locus_tag="VFQ79_17135"
     CDS             4281..>4810
                     /locus_tag="VFQ79_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0141451.1"
                     /translation="MSVRTLEIDGKAVTGVAGQTIFSVAWDNGIHIPRLCHVGGVSDV
                     GACRLCMVEVEGQRKLLAACMTPVEEGMVIRTNTERLVAHRRLIVELLLAERNHVCAV
                     CVVNGACELQDLAASLGIDHVRLRYRFPTLSVDTSHERFGLDHNRCVLCSRCIRVCDE
                     VEGAHTWDFSGRGANSL"
BASE COUNT          943 a         1372 c         1541 g          954 t
ORIGIN      
        1 atcgcgctga tcggatttcg caatgccggc atcatagtga ccagcgagac tacagcggtg
       61 gcgatgggga atctacgcga tccccatacg ctactctcga tttttggcct gttgctgatt
      121 tccggtctgc tggtgtgggg agtgcgcgcc gcgatgctgg tgggtgtgct ggcgacaacg
      181 gcgcttggcg ctatcaccgg cctggtgcag tggcgacccc aggcctatag cttctccgat
      241 atctcggcaa cggcttttca gttggatatc gccggagcga tccggctcgg cttactcgaa
      301 atcatcttcg tcttcctgtt cgtcgacctt ttcgataacg tcggaacgct ggtcgcggtg
      361 gggaaaaagg cgcggctgtt tgacgaagcg caccagattc cccgcatcaa ccgcattctc
      421 tattcggatg caacggccac catggcgggc tcgctcaccg gaacctccac cgtggtgagc
      481 tacatcgaaa gcgccgcggg ggtggtagcg ggaggccgaa ccggcgtccc cgcggtggtg
      541 gcgggactcc ttttcctggt agcgttattc atcgcaccgg ccatgggagt agtgcccgcc
      601 gcggcaacgg ctccggcgct gattctggtt ggttccttca tgctgaccca cgtagcggaa
      661 atccagtggg acgatccggt ggtggcgatt ccggctttcc tcacgattac gacgattccg
      721 ttgtctttca gcattgccaa tggcttgtcg ttcggcttca ccgcctacgc tctgctgcgg
      781 ctggccaggg gagagttccg gaaggtgaac tggcttgtct ggctgctggc ggcgctcttc
      841 atcgtgcgat tcgcgtatct tggcggcggc tagcgtggct atcgacgtgc actccgaaag
      901 gcatgcacgg acatggaaac gcgggcgggc ctcgtcccag actggttttc tgggaactga
      961 caactggctg taatctcaga tgcatacact gccgcgccag cgcgacggag ttgatgtctc
     1021 cagacgacct gaactaccag gaaagctgtg acgtcatcga tcagattgcg gaatatgccc
     1081 cactcatcct ggttttgagc ggtggagaac ccctttggcg gcgcgatgtg tttgatttgg
     1141 cgcaccgtgc gcgaagcaag aacatccgcg tggctctggc aacgaacggc acgctggtgg
     1201 acgaagccat ggcgcagcga atccaagatg caggtattca aagagttgcc atcagtttag
     1261 acggtgcgga ccgggccacc catgacaatt ttcgcggcca ctcgggggcg tttgatgccg
     1321 ccattcgtgg cctgcggtgt gtgcaggacc tggggatttc tacacagatc aacaccaccg
     1381 tttcgcatca caacgtgcat cagcttcccg atatcatgaa gctggccgaa acgctcggcg
     1441 tcaatgcatt tcatatcttt ctgctggtac cggtgggctg cgggctgacc atcagcgaag
     1501 accagtccgt gaaaggcagg gaagccgaac agatcctgga atggttttac gagcggtcgc
     1561 tcgaatcgac tatggaattg aaggccacct gcgctccaca gtattaccgc atcgtgcgcc
     1621 agaagcgcgc ggaagccaag cgggcgggct tgagtgtgcc ggacctgccc actcatggga
     1681 tgaatgccgt tacgcggggc tgcctggcgg gccagggcgt ttgcttcata tcgcatcgcg
     1741 gcgaagtgta cccttgcggc tatcttcccc ttgccgccgg tgatcttcgc cgccagcgtt
     1801 tccgcgacat ctgggagcgc gcgaccgtgt tcgcgacgct gcgggattcc ggcaatctcg
     1861 acggcaagtg cgggttctgc gaattcaggc aggtgtgcat gggctgccgg gccagggcgt
     1921 atggagtcta cggaaatttt ctggccgaag agccgttctg cgagtaccaa ccccgcacgg
     1981 gagtgcacgc ctgacggtgt gtgtccaccg tcagaaccgg gggcatgata ttctggagtt
     2041 tgatactgta ttatcttaaa taattcatta aagtccattt aaaaatcgga gtgaggaaag
     2101 aagcgcgacc cgcatgccag tcgttaccaa acccgagacc ccggcggtaa aagacaaccg
     2161 cgaacgtttc ctggagcggg cgatgtcccg ccatcattat tcgggcgatg cgttgataga
     2221 agttcttcat acggctcagg aattatacgg atttcttacg cccgccctac tcaaaaccat
     2281 cgcccggaaa ctgcgccttc cgcccagccg cgtgttgggg gtggcaacct tttatcattt
     2341 cttctcgctg gagccgaaag gcgaacattc catgatcgtc tgcacgggca cggcttgtta
     2401 tgtagccgga gcccaggggc tgatgcgtat tctcgagcac cggtgcgcga aggccggcgg
     2461 gacgagtccc gacggcaaga tgagtgtgca ggcggcgcga tgcatcggtt catgcggact
     2521 ggctcccgcc gtgatttacg acggaaagat cctgccgcgc gtaactccgg atcaactgaa
     2581 cgcggaactg gatcggattg gtgtgagatg accttcgaag atctgctgga acttcaggcc
     2641 aaagagatcg cacgccagca agggctgcgc gagcgcatct actgctgcac cgccgcgggc
     2701 tgcatctcct cgggtgcgga cgcggtcaaa aaagccatcg acgaggagat caaagcccgt
     2761 ggggtgggtg ccgacgtgga gtgctgcgga accggctgca tgggcctgtg cagcaagggg
     2821 ccgctggtgc gtgcgcagtc cgacggagcc atttacgcgg cgataacgcc ggaggatgcc
     2881 ggcgcactgg ttgcgggaga agatgccaac ctgctggcgg gccggaaact ggacccgcaa
     2941 gccgatttct tcaccaggca ggagaagatc gtgctggcaa acagcggcac ggtcgacccc
     3001 gaaaagcttt cggactacat cgcagccgga ggataccagg cactggcccg ggccgtgatg
     3061 gaattgaccc cgcaacaggt tattgaggaa gtgaagcgaa gcgggctgcg gggccgcggc
     3121 ggtgcgggct acagcaccgg cctcaagtgg gagctggtgg ccaagcagcc ggtggatacc
     3181 aagtatgtcg tctgtaacgc cgacgagggc gatcccggcg ctttcatgga ccgcagcatc
     3241 ctcgaaggcg atccccaccg catactggaa ggcatggcta tcgccggtta cgccgtgggc
     3301 gcgcaacagg gctacatcta tgtccgcgga gagtacggac tggccatcca gcgcctgcaa
     3361 gcggccatca agcaggctga gcgcgagcgg ctgctcggca gccggatctt cgaaaacagc
     3421 ttccagttcc gtatcgacct gcggatcggg gctggggctt ttgtttgcgg cgaggaaaca
     3481 gcgctgatcg cttccatcga gggcaagcgt ggacagccgc gccagcgtcc gccctatccg
     3541 ccggaatccg gcttgtgggg caagcctacc ctgatcaata acgtggagac gttcggcaac
     3601 atcgctccca tagtggcgaa gggcagcgaa tggtttgcga ccattggcac ttctgccagc
     3661 aaaggaacca aggtcttcgc tctggcgggc aaaattgaga acaccgggct cattgaagtg
     3721 ccaatgggta ccagcctccg caccatcatc ttcgacatcg gcggagggat tccgggggga
     3781 aaggaattca aggcggcaca gaccggcgga ccgtcgggag gctgcattcc accgcagttc
     3841 ctggacgtac cggtagatta cgagtcgctc cacaagatcg gctccattat gggctcgggc
     3901 ggcctcatcg tgatggacga tacgtcgtgc atggtcgacg tggcgcggtt cttcatggat
     3961 ttttgcatgg acgaatcgtg cggcaagtgc ataccgtgcc gggcgggaac cgtccagatg
     4021 cacgatatcc ttcagcgcat tacgaaaggc gaggcgaacg ataacgacct cgttgtgctc
     4081 gaacaattgg ccaagctgct gcgggagacc agtctttgcg ggctcggcca gacagcgccc
     4141 aaccccgtga tgagcacgct caattacttc tccggagagt accaggcgca cattaaggat
     4201 caccggtgcc ccgccggcca gtgcacgctc gacggagaag aggcggccgc gacggtgaac
     4261 ggaaatggga aggttcaggc atgagtgtca gaactctcga aatcgacggt aaggccgtaa
     4321 cgggtgttgc cggccagacc attttcagcg tggcgtggga caacggaatc cacatccccc
     4381 ggctgtgcca cgtcggaggc gtgtccgacg tcggcgcctg ccggctgtgc atggtggagg
     4441 tggaaggcca gcgcaagctg ctggccgcct gcatgactcc ggtggaagag ggaatggtca
     4501 tacgcaccaa caccgagcgg ctggttgccc accggcggct gattgtcgag ctgctgctcg
     4561 cggaaagaaa ccatgtctgc gcggtgtgcg ttgtgaatgg agcgtgcgaa ttacaggacc
     4621 tggccgcttc gcttggcatc gaccatgtcc ggcttcgcta ccggtttccc actctttcgg
     4681 tggacacgtc gcacgaacgc ttcggcctgg atcataaccg gtgcgtcctt tgcagccgct
     4741 gcatccgggt ttgcgacgag gtggaaggcg cgcacacgtg ggacttctcc ggacgcggag
     4801 ctaattctct
//
LOCUS       DASTMK010000396         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_466500, whole genome shotgun sequence.
ACCESSION   DASTMK010000396 DASTMK010000000
VERSION     DASTMK010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466500"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFQ79_17140"
     CDS             <1..378
                     /locus_tag="VFQ79_17140"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0141452.1"
                     /translation="LKSNRGNVVLVNLWATWCAPCREEMPALVALQSKLASKGLRLIT
                     VSADEPEDEASARQFLSEQKVPSPAYIKRVENDEKFIESMDPKWSGALPASFLYDRNG
                     RKARAFIGEVDMKELEAAIRKLL"
     gene            complement(416..3994)
                     /locus_tag="VFQ79_17145"
     CDS             complement(416..3994)
                     /locus_tag="VFQ79_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141453.1"
                     /translation="MRLTSRLATCIVLPLVSCWTSLAQESRGTIVGRVIDSSQAAIPD
                     ASVEVTNKAMGTKLSVKTNETGLYQAPYLIPGLYQVAVEVPGFKRFIRDNVAVQVNDR
                     LELDITLEVGTAEQTVTVTAETPLLSTTTGSMGAVVDGRRISELPIPHGNPYFLIALA
                     PGVTFARDARLDRPFEPTHIVGYAMDGTRANRSDVTLDGAPSTATANAGEVISSYVPP
                     ADIISEFRVQTATFDASFGQTEGGVTNISIKSGTNALHGTVNYTKMAPELFANDFFAN
                     ANRIPRADLTYDRWGGTAGGPVYLPKLYDGRNRTFFMWGYEGIHEARPRNNGTNTVPT
                     VENKLGDFSALLNLNSNYQIYNPFTRRSIGGGRYQQDPFPGNIIPSSLINPVARNILA
                     YFPDPLTPGNPDGTNNYSRSDLMERAKYYTHTVRIDHTLTEKQRIFVRGSVYRRDSTY
                     NNYFDNLATGSLFQFLSRSFVGDHVYNLTPTVVMNVRYGYNRFIRVDAPNPESQGFDL
                     TQLGFPAEYNNAISADIRRFPRLDFPGGTYQGTGFGGDYRPNDTHSLNFTVQKAQGRH
                     FIKSGMEFRAYRENSRPLGNDQTGRFSFDSTWTRGPLDNSPNSPAQLGQSFAAFLLGL
                     PSGSNSLLNRPAGYAEQSTSWGFFLHDDWTVNSKLTLNIGLRWEFEGALTERFNRSVR
                     GFDVNAAQPFEAQARANYARNPTPEIPPEQFNASGGLTFAGVNGLPRGLYNTPKRNLM
                     PRFGLAYKLDDKTVLRAGYGIFFGFLGQRRSDVIQSGFTRATPFNASDDQGLTFSSTL
                     SNPFPNGILEPLGAAQGAETFVGQAITFFNEDPRMPYMQRWQFGIQRELPAGFVTELN
                     YVGNRGTNIEIQRNLNVTPQRYLSRSPVRDNQTINYLSANLPNPFAGLLPAGAIGVFT
                     STSIARERLLRPYPHFDTVTSTTYEGYSWYHSLQARIEKRFSKGYTLAANYTFSKFMQ
                     ATELMVPDDLRPFEGVSDMDYPHKVAVSGIYELPFGDRRRWLAGGNRVVSMLVGGWQI
                     SGMFTYQSGPPILWGATTGSSTFTGNNLTINSVIFNGDINDIRLSGSERSLSRWFNTD
                     AGFERNSAQQLDTARQIRTFPLRFGFLRGDNISNFDLALFKNARVAEGKEIQLKGEFL
                     NAFNHPQFPTPAGNAFNPANQTFGQIVASNQANYPRRIQLSLRFLF"
BASE COUNT          879 a         1205 c         1355 g          929 t
ORIGIN      
        1 ctgaagtcga accgtgggaa tgtagtgttg gtgaacttgt gggccacctg gtgcgcgccc
       61 tgccgggagg aaatgcccgc cctggtcgca ctgcaatcga aactcgcatc gaaaggcctg
      121 agactgatca cagtttcggc agacgaaccg gaggatgaag cttccgctcg ccagtttttg
      181 agcgagcaaa aggttccctc tcctgcttac atcaaacggg tcgagaacga cgagaagttc
      241 atcgagtcca tggatcctaa gtggagcgga gccctgccgg cgtccttcct ttatgaccgt
      301 aacggccgga aggcccgtgc tttcatcggc gaagtggata tgaaagaact ggaagcggcc
      361 atccggaaac tgctgtaatt gggatggccg cattcccggt ccgaaaacta ccggatcaaa
      421 atagaaaccg caaactcaac tgaatgcgcc gcgggtagtt ggcctggttg gacgcgacaa
      481 tctgaccgaa agtctggttc gccggattga atgcgtttcc ggccggcgtc gggaactgcg
      541 ggtgattgaa cgcgttcagg aattcacctt tgagttggat ctctttgcct tccgccaccc
      601 gggcattttt gaacagcgcc aggtcgaagt tgctgatatt gtcgccgcgc aggaacccga
      661 agcgcagtgg gaaagtccgg atctggcgcg ccgtatccaa ttgctgcgcg gagttccttt
      721 cgaaaccggc atctgtgttg aaccatcgtg agagactgcg ttcgctgccg gatagccgga
      781 tgtcgttaat gtcaccgttg aagatcacgc tgttgatggt taggttgttg ccggtgaaag
      841 tggaactgcc ggtcgtcgcg ccccacagaa tcggcgggcc gctctggtaa gtaaacatac
      901 cggagatctg ccaaccgcca accagcatcg agacaacccg gttaccgccg gccagccagc
      961 ggcgccggtc tccgaacggc agctcgtaaa tgccgctgac ggctaccttg tgtggataat
     1021 ccatgtcgga aaccccctcg aacggccgca ggtcgtccgg caccatcagt tccgtagcct
     1081 gcatgaattt ggagaacgtg tagttggccg cgagggtata gcccttcgag aatcgcttct
     1141 cgatgcgggc ttgcagtgag tggtaccagg agtagccctc gtacgtggtt gacgttacgg
     1201 tgtcgaaatg cggatacggc cgcagcagcc tttcgcgggc gatgctggtg cttgtaaaga
     1261 ccccgatagc gccggcaggc agcagaccgg cgaacgggtt gggcagattg gcgctcaggt
     1321 aattgatggt ctggttgtcg cgcaccgggc ttctgctcag ataccgctgg ggggtgacgt
     1381 tcaggtttcg ctgaatctcg atgttggttc cgcgattgcc gacgtagttt aattcggtga
     1441 cgaatcccgc cggaagttcc cgctgtatcc cgaattgcca ccgctgcatg tagggcatgc
     1501 gcggatcctc gttgaagaag gtaattgctt ggccgacgaa ggtctccgct ccctgtgctg
     1561 cccccagcgg ctccaggata ccgttcggga aaggattgga cagagtggag ctgaaggtta
     1621 gcccctgatc gtccgatgcg ttgaacggcg ttgcgcgggt aaacccgctc tggatgacgt
     1681 cgctgcgccg ctggccgaga aaaccgaaga agatgccgta gccggctcgc agtacggttt
     1741 tgtcgtcgag cttgtaggcg aggccgaagc gtggcatcag gttgcgttta ggcgtgttgt
     1801 agaggccccg cggcagcccg ttgactccgg cgaaggtcag tccccccgag gcgttgaact
     1861 gttcgggcgg gatttccggc gtcgggtttc gcgcatagtt ggccctcgcc tgtgcttcaa
     1921 acggctgggc ggcgttaacg tcaaagccgc gcacgctgcg attgaaccgt tccgtaaggg
     1981 cgccctcaaa ctcccatctc agaccgatat tgagtgtgag cttggagttt acggtccagt
     2041 cgtcgtgcag gaagaacccc cacgacgtgg actgctcggc atagcccgcc gggcggttga
     2101 gcaagctgtt ggaacccgag ggcagaccca gcagaaaggc cgcgaacgac tggcccaatt
     2161 gcgcgggcga gttcggggag ttgtccagcg ggccgcgagt ccaggtggag tcgaaactga
     2221 agcggccggt ctggtcattt ccgagaggcc tgctgttttc ccggtaagcg cggaattcca
     2281 tgccactctt gataaaatgc cggccttgcg ccttctggac ggtgaagttc aaagagtgag
     2341 tatcgttagg acggtaatcg cccccaaatc ccgttccctg gtaggtgcca ccgggaaagt
     2401 cgaggcgcgg aaaacgccgg atatcggccg agatggcgtt attgtactcg gcgggaaacc
     2461 cgagttgcgt cagatcaaag ccctgcgatt ctggattggg agcatccaca cgaatgaaac
     2521 ggttatatcc gtagcggacg ttcatgacca cggtcggagt caggttgtag acgtggtcgc
     2581 cgacgaaaga gcgcgatagg aactggaaca gcgaccccgt ggccaggttg tcgaaatagt
     2641 tgttatacgt gctatcgcgc cggtacacgc tgccgcgaac gaaaatcctc tgtttttccg
     2701 tcagcgtgtg gtcgattcgc actgtatggg tgtaatattt cgcccgctcc atcagatcgg
     2761 atcgcgagta gttgtttgtg ccatcggggt ttccgggtgt gagcggatcc ggaaagtacg
     2821 ccagaatatt tcgcgcaacc ggattgatca gcgaactggg gatgatgttg cccgggaacg
     2881 ggtcctgctg gtaccggccg ccgccgatag aacggcgcgt gaacggattg tagatctgat
     2941 aattcgagtt caagttcaac agggccgaga aatcgccgag cttgttctcc actgtcggca
     3001 ccgtatttgt gccgttgttg cgcggccggg cctcgtgaat accctcataa ccccacatga
     3061 agaaggttct gttccggcca tcgtaaagtt tcggcagata aacggggccg cccgccgtgc
     3121 cgccccaccg gtcataagtg aggtcggcgc gcggaattct gttggcgttg gcgaagaaat
     3181 cgttcgcaaa cagttccggc gccatcttcg tgtaattcac ggttccgtgc agcgcattgg
     3241 ttcccgattt gatgctgatg ttggttacgc cgccttcggt ctgcccgaag ctggcgtcga
     3301 aggtagcggt ctgtacacgg aactccgaga tgatgtccgc gggcggaaca taggatgaaa
     3361 taacttcacc cgcgttcgcc gttgcggtgc ttggcgcgcc gtccagtgtt acgtcgctgc
     3421 ggttggcccg agttccgtcc atggcgtagc cgacgatatg agtcggctca aacggccggt
     3481 ccagccgggc gtcacgcgcg aaggtaacac ctggtgcgag ggcaatcaga aaatacggat
     3541 tgccgtgcgg gatcggaagt tcggaaatac ggcggccgtc cacaacggcg cccattgagc
     3601 cggtggtggt gctgagcagc ggcgtttcgg cggtaaccgt caccgtttgt tccgcggtgc
     3661 ccacctccag cgtgatatcc aattccaggc ggtcgttgac ctgtaccgcc acattgtcgc
     3721 gaatgaacct cttgaacccc ggcacttcca ccgcaacctg gtacaagccg gggatcaggt
     3781 acggggcctg atagagacca gtctcattgg ttttcacaga cagcttcgtt cccatcgctt
     3841 tgttggtgac ctccaccgaa gcatccggga tggctgcctg cgagctgtca atgactctgc
     3901 ctacaatggt tccgcgggat tcttgagcga gtgacgtcca acaggaaacc agcggcaaaa
     3961 cgatacaagt cgcaagacgc gaagtgaggc gcatcgttcc ccctccccac ggccacatgg
     4021 gccgttgaga caagactatc ctgcgtcaag aataaccgtt ttgcttgtca agcgcaccag
     4081 tatcagcggg caggtgcagg agtttacttc tgaaaccgcc ggctaaacac gacgccgcct
     4141 ccgtttgcgt atcccgataa ccactccctc gcggtcgtgg ctccctcgaa aataatgcct
     4201 gactcgcgaa tcaggctgcg ggatggaggg tgacctcgta tccgagcctt tccagtcgct
     4261 tgacggaacg tttcaggagg ctctccttct ggcgcagatc aaagtatagg gtgcccatct
     4321 cccggtactc ggtttggtca cgcagaacgt attggcgacg gccaggag
//
LOCUS       DASTMK010000397        11657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_628031, whole genome shotgun sequence.
ACCESSION   DASTMK010000397 DASTMK010000000
VERSION     DASTMK010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628031"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFQ79_17150"
     CDS             complement(<1..798)
                     /locus_tag="VFQ79_17150"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0141454.1"
                     /translation="MQTSLIATPIRDVETEALILLSFEKEDREKAELFASVNSATGGW
                     LAEIYDTAEFTGKPLTFALLHRPPGMKSRRVLAVGAGKRDRFDAAELRKAIAAGVRHL
                     KGKGCRSIAVVLDPLWAAPEYVSAAVEGAITGDFEPSRYKTAKEEECALDSLTVVAPS
                     TGVEWQQALGRGRILAEAQNFTRELVNEPANRMSPMVLADRARRLAEEQGLDFELLDR
                     DRMAQLGMGALLGVAQGSEEPPALIILRYKPATPASTNAHLGLVGKGV"
     gene            complement(810..1277)
                     /gene="smpB"
                     /locus_tag="VFQ79_17155"
     CDS             complement(810..1277)
                     /gene="smpB"
                     /locus_tag="VFQ79_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU0141455.1"
                     /translation="MATQSGFKILSDNRQAGHNYHLMDRFEAGLVLTGTEVKSARTGK
                     IQLKDSYAEVVSNEAWLVNAHISHYTHGNRENHEPVRRRKLLLHRREIDKLAGKTREK
                     GLSLIPTKIYLKEGRIKVELALAKGKKLHDKREAERTREQQAEARAALGRRTA"
     gene            1387..3867
                     /locus_tag="VFQ79_17160"
     CDS             1387..3867
                     /locus_tag="VFQ79_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEU0141456.1"
                     /translation="MDLSRRLLSLLLALLLAAPATVEARNRKGDKLLKEGQQAEQKKN
                     YDEALILYEKAVLEDPSETAYQLALRRVRFQAAQAHVDKGKQLRDAGNLEEALKEYQH
                     AYAIDPSSMIAEQELRRTFQMMERNKKLSAETGRELTPKEQGMTPSQAADAKLEERIS
                     GYQPIPELRPITPQITSLKMNNQPPRVLFETVAKLAGINVIFDPEFIQQNTRPSNLDL
                     SNTTLQQALDHLAVLTNAFWKPLSANTIFVTQNNPTKRRDYEDQVVKVFYLQNVTNVQ
                     ELQEIAVTVRSVLELRRAFTYNAQNAIVIRAEADRIALAEKLLRDLDKPKSEVVVDLV
                     VLEANRTKTRDLAAALQSAGGAIGLRAPVNFTPRNPVLSGNDGNGDDNGNDDGDDDNG
                     NSNTSGLGGILGSSSSGNTTQEQAISLARIGRISTNDFSITLPGALFQAMLSDRSTRV
                     IQSPQVRSIDNAKAELHIGDKYPYATGSFQPGIGSVGVSPLVSTQFQFADVGVKLVMT
                     PKVHGSDEVSLHVEMEISSVRDRIDVGGLSQPVIGQRRVVHDIRVKEGQVTLLGGLMQ
                     NQDTNSVSGLPGIASLPGLKWLFGSESVEKNQGELLIALIPHIVRSQELSENNLKGIS
                     SGTEMNYKVTYSSPTPEKPTAPVEAPKPEAPPAAPVPGAAPEVKPEAPPVEAPKPETP
                     AAPAAPAATALSFNPPAVEARLGAEIPVSIQIQNVTDLFSAPVRLRFDPKRLRLNEVT
                     RGTLLSGDGQNVIFTRNIQNDVGELTVNLNRLPGSAGVSGSGTLLNLTFQAIGVGQAQ
                     VTVVDGGLRNSQMLSIQAGNPILVINVK"
     gene            3877..4341
                     /locus_tag="VFQ79_17165"
     CDS             3877..4341
                     /locus_tag="VFQ79_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU0141457.1"
                     /translation="MRRWRQRGLTLVELIVAFTILLTLTSMAVPMARMKVKREQEREL
                     RMALREIRAAIDKYKDAADLQQIGPTKMGSENYPESLEILVEGVSKTGAVDTKVKFLR
                     RIPKDPMTNSYDWGLRSVQDDPESIAWGGQNVFDVYTKSLDKAADGTPYSKW"
     gene            4316..4720
                     /locus_tag="VFQ79_17170"
     CDS             4316..4720
                     /locus_tag="VFQ79_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU0141458.1"
                     /translation="MVHPIRSGRRGFTIVELMIVLTIVGILVSIAIPIYQKSIIRAKE
                     SVLKSNLFTLRTVIDEYTYDKQKAPQSLQDLVNDGYLRQVPVDPMTGSDSTWKIIMED
                     AMNSIDQTEPGIFDVKSGSDRMSLEGTPYSEW"
     gene            4779..4868
                     /locus_tag="VFQ79_17175"
     tRNA            4779..4868
                     /locus_tag="VFQ79_17175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4813..4815,aa:Ser,seq:cga)
     gene            complement(4945..5337)
                     /locus_tag="VFQ79_17180"
     CDS             complement(4945..5337)
                     /locus_tag="VFQ79_17180"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141459.1"
                     /translation="MDLKDSQLIREVLLSFWKIHILHHAAEGTVYGQWMIGELRRHGY
                     DISPGTLYPLLRRMESHGWLKPIRATPVNSHSRREYRLTKLGTDALDLIRAQLKELYR
                     EVFEEKQGGRRRSKKSKGKPQSGSHTHD"
     gene            5626..7011
                     /locus_tag="VFQ79_17185"
     CDS             5626..7011
                     /locus_tag="VFQ79_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0141460.1"
                     /translation="MRFTIITRTLGVGVLLASIGPGTLLGQSYTIGQAVADAGDRYPA
                     TRVSIEQVSAAAQAINLARTAYLPRVDFLSQVNRATRNNVFGMTLPQATLPAISGPPL
                     PENDMTSVFGSTVGFTVTWEPFDFGARQANVRYAEAGRRVAETGVSRTRFEVTTAAAD
                     AVLTVIAAEQTAIAAQAGVERSRVLLEMVQAQVRAQLRPGADASRARAELALAQNQLI
                     RAQSAIEVAKANLTQFVGGDPTTISVQTEPLLKLPDPSAGAGSLMPNHPVLQQQRSLI
                     EQIQANEQVLQRLYYPRFSLQGSNYARGTGAYANGSTLGGLNGLGPNIYNWGVGLSVT
                     FPLFDLSSIRSKREIERYRERAESARFDQYQQDLAAAVSSARANLDGARRVAQNTPIQ
                     LDAARATEQQMSARYKSGLGTLIELAEAQRLVTQAEIDDALARLGVWRALLQAAVAQG
                     NLEPFLQEAGK"
     gene            7041..10163
                     /locus_tag="VFQ79_17190"
     CDS             7041..10163
                     /locus_tag="VFQ79_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0141461.1"
                     /translation="MRRPITILVVVMAVALASILAIRRMKMDIFPSLGSPTIYVAQPY
                     GGMDPSQMEGFLTYYYEYHFLYITGIEHVESKSIQGAALMKLVFHPDTNMNQAVAETV
                     GYVNRSRAFMPPGAVPPFIVRFDAGSVPIAQLVFSSPNRSPGEMQDFAINRVRPLFAT
                     LPGVSAPPPFGGNQRTIIVRIDPDRMRAYQMSPEEVIAAVNKASAVLPSGLVRIGNDA
                     YIASTNATVGGNLDEFMNTPVRTGAGPTVYIRDVGLVENGTDILSSYAHVNGRRTVYI
                     PVTKRSDASTLDVIQRVREALPRMQAAVPEDVEVRLEFDQTGYVSRALNSVVYEALIG
                     AALTGLMVLLFLRDWRSSLVVVATIPFALLAAVVCLWAAGQTINIMTLAGLALAVGIL
                     VDEATVLIESIHSHMTQGDSKVWAVMGASAKTAIPRLLAMLCILAVFVPSFFMAGVGQ
                     QLFIPLSLAVGFAMISSYILSSSLVPVLSTWILRRAHCEAETGLFHWLREIYTKYLRF
                     TLKLRWPVAAAYVFGCFGLAWLLLPKIGTEIFPTVDAGQFQLRLQAPTGTRIEQTERI
                     TLQAMDLIGETLGRDNVRITTSFIGVQPASYPINTIYLWTSGPHEAVLLVALKPEAKL
                     RGEELKEELRARFRKRMPDVRISFEAGDIVARVMSFGSNTPIEVAVQGPNIAQNRNHA
                     EKVRTELAKLPFLRDLQYAQPLDYPTLQVDIDRDRAGQFGMTAAGVARSLVAATSSSR
                     FVDPNFWRDPNSGNAFQIQVEVPQHEMASIEDMRALPITSNGSRALLGDVANIKVTRS
                     LGLVERYNMQRVVSLTANISGKALGQVVADIQRATARAGDAPRGSTVAVRGQIPALNE
                     TISGLQVGLGLAIAVIFLLLAANFQSFRLAIAVITTIPAVLVGVLLMLLITGSTLNVQ
                     SFMGAIMSSGIAVANSILLVTFAEFSRHAGSNAVDAGIEGGRGRLRAILMTATAMIAG
                     MIPIALGIGEGSEQTTPLGRAVIGGLLFATFATLTVLPAFYAILQRKATPVTSSLHPL
                     DAEGRFYEPR"
     gene            10150..11238
                     /locus_tag="VFQ79_17195"
     CDS             10150..11238
                     /locus_tag="VFQ79_17195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0141462.1"
                     /translation="MNPGKFFVALAVTGVLSAQNVPVVKVESKALSRTVLLTGEFLPF
                     QTVDLHARVQGFVEKVLVDRGSIVKEGELLVTLSAPEMEAQVAEAEAKVKTAESSASE
                     ARAKLVAAESTYKRLKEASATEGAIAGNELVIAEQSAEALRDTVRASESAITAAKASL
                     AAVQKMQQYLSISAPFPGVITERLAHPGALAGPSTGPLLHLEQVSRLRLVVAVPEANF
                     AGVRPGTKVPFQVSAYPTRMFEGAVARIARSIDQKTRTMSVELEVPNASGTLAPGMYP
                     RVNWPVRSTERALVVPSTSVVRTTERVFVIRVKDGRAEWVDVQVGARESGQFQVFGSL
                     AEGDMVVRNGSDEIRDGSQVSVTTAKPG"
     gene            11283..11624
                     /locus_tag="VFQ79_17200"
     CDS             11283..11624
                     /locus_tag="VFQ79_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141463.1"
                     /translation="MYEILMGATSVIAGAIASIVGFGIGSILTPLLSLRIGTRLAVAV
                     ISIPHLFATFVRFVATATAIGLVVDAVRMPVYFVTAGDEVLRMPGVIISSTVGGDRHS
                     EPDSQLRRELP"
BASE COUNT         2432 a         3364 c         3476 g         2385 t
ORIGIN      
        1 gacgccttta ccaaccaggc ccaggtgcgc gttcgtggac gcgggagtgg caggcttgta
       61 ccggaggatg atcagcgccg gcggctcctc gctgccttgc gcaacgccga gtagcgcgcc
      121 catacccaac tgtgccattc gatcccggtc cagcagttcg aagtccaggc cctgttcttc
      181 ggccaaccgg cgcgcgcggt ccgccagcac catcgggctc atccgattgg cgggttcgtt
      241 caccagttcg cgggtaaagt tctgcgcctc ggcaaggata cggccgcggc cgagcgcctg
      301 ctgccattcg acccctgtgg agggagccac aaccgtcagg ctgtcgagcg cgcattcctc
      361 ctctttcgcg gtcttgtacc ggctgggctc gaaatcgcct gtaatcgcgc cttccactgc
      421 cgcggacacg tactccggag cagcccaaag ggggtccaga accaccgcga tactccggca
      481 gcctttccct ttcaggtgac ggacgcccgc cgcaatcgcc ttccgcaatt cggcggcatc
      541 gaaacggtcc cgcttgcccg cgcccacggc gagcacacgg cggctcttca tccccggcgg
      601 cctgtgcagc agggcaaacg tgaggggctt tccggtaaat tcggcggtgt catagatttc
      661 cgcaagccaa ccgccggtag cggaattgac ggacgcgaac aattccgcct tttcccggtc
      721 ctctttttca aaggagagaa gaatcagcgc ctcggtttcg acgtcccgaa tgggtgttgc
      781 gatcagtgaa gtttgcattc tatttataat caggcggttc gtctgcccaa cgcagcgcgc
      841 gcttccgcct gctgttcccg tgtgcgctcc gcttcccgct tgtcgtgcag ttttttgccc
      901 ttggccaggg cgagttccac tttgatcctg ccttctttga ggtaaatttt cgtgggtatc
      961 agcgagagac ccttctcgcg ggtcttacct gccagtttgt cgatttcgcg gcggtgaagc
     1021 agtagcttcc ggcgccgcac cggttcgtgg ttctcgcgat taccgtgagt gtaatggctg
     1081 atatgggcgt tgaccagcca ggcctcgttc gaaacgactt ccgcatagct gtctttgagc
     1141 tggattttgc cggtccgtgc ggatttgact tccgtgcccg tcaggacgag ccccgcctca
     1201 aaccggtcca tcaaatggta attgtgccct gcctgccggt tatcgctaag aatcttgaag
     1261 ccagactgag ttgccactcg aaaaatgccc tcaacgctgg aaaaccgaga tacgtcaatc
     1321 gtttgtgtgg tgtttccaga cggatgccgc acactatatt agcataaacc tctacgactc
     1381 aataagatgg acttatccag acgcctcttg agcctgctgc tcgccctgct gctcgccgct
     1441 cccgctacag tggaagcccg aaatcgcaag ggcgacaaat tactgaaaga gggccagcag
     1501 gcagagcaga aaaagaacta cgatgaggcg ctgatcctat atgagaaggc ggtgctggaa
     1561 gatccgtccg aaacagccta ccaactggcg ctgaggcgcg tccgttttca ggcggcacag
     1621 gcccacgtcg acaagggcaa gcagttgcgc gatgccggaa acctggaaga agcgctgaaa
     1681 gagtatcaac acgcctacgc catcgacccg agttccatga tcgccgagca ggaactgcgc
     1741 cgcacgttcc agatgatgga acgtaataag aagctcagtg ccgagaccgg ccgcgagttg
     1801 acgccgaagg aacagggcat gactccgtcc caggcggcgg acgccaaatt ggaagagcgt
     1861 atctcgggat atcagcccat tcccgaactg cggcccatca caccccagat cacgtctctc
     1921 aagatgaaca accagccgcc gcgggtcctg ttcgagacgg tcgcgaagct ggccggcatc
     1981 aacgtgatct tcgacccaga gtttatccag cagaacacca ggccctccaa cttggacttg
     2041 agtaatacga ccctccaaca ggcgctggat catctcgccg tgttgacgaa tgctttctgg
     2101 aagccgcttt ccgccaatac gatcttcgtc acgcagaaca atccaaccaa acggcgtgat
     2161 tacgaagacc aggtggtgaa ggtgttctat ttgcagaatg tcaccaacgt gcaggaattg
     2221 caggaaatag ccgtgacggt gcgaagcgtg ctggagttgc gccgcgcctt cacctataac
     2281 gcgcagaacg ccatcgttat ccgcgccgag gccgatagaa tcgcgctggc ggagaagctg
     2341 ttgcgcgatc tggacaagcc gaagtccgag gtagtggttg acctcgtcgt gttggaggcc
     2401 aatcgtacga agacccgcga cttggccgcc gctttgcaat cggcgggcgg cgcgataggg
     2461 ctgcgtgcgc cggtgaattt cacaccgcgc aacccggtgc tttcgggaaa tgatgggaac
     2521 ggcgacgata acggcaacga tgacggagac gatgacaacg gaaactcgaa tacaagcgga
     2581 ttaggaggga ttctcggctc ctcgagcagc ggcaacacta cgcaggagca ggcgatttcg
     2641 ctcgcgcgga tcggcagaat ttcaaccaac gatttttcca tcactctgcc gggcgcatta
     2701 ttccaggcca tgctttcgga ccgttccacc cgcgtgatcc aatcgccgca ggtgcggtcg
     2761 attgacaatg ccaaggcgga gctgcacatc ggagacaaat atccctatgc aacgggcagc
     2821 tttcagccgg gcattggctc ggtcggcgta agtccgctgg tgagtaccca attccagttt
     2881 gccgatgtgg gcgtgaaact cgtgatgaca ccgaaggtgc atggcagcga tgaggtttca
     2941 ctgcacgtcg aaatggagat ttcgagtgtg cgcgaccgca tcgacgtcgg gggactgtcg
     3001 caaccggtta tcggacagcg tcgcgtagtg cacgatatac gggtgaagga aggccaggtg
     3061 acgctgctcg gcgggctgat gcagaaccag gacaccaatt cggtatcggg tctgccggga
     3121 atcgccagtc taccgggatt gaagtggctg ttcggcagcg aaagcgtgga aaagaatcag
     3181 ggcgaattac tgatcgcgct gatccctcac atagtacgat cgcaggaact cagcgaaaac
     3241 aatttgaagg gtatctcatc gggaacggag atgaattata aagtgaccta ttcgtcaccc
     3301 acccctgaga aacccaccgc tcctgtagag gctccaaagc ccgaagcgcc tccggccgcc
     3361 cctgtaccgg gtgcggctcc cgaagtgaaa cccgaagctc caccggtgga ggcgccaaaa
     3421 ccggaaacac ctgctgcgcc cgctgctccc gcggctacgg ccttgagctt caacccaccg
     3481 gcggtggaag cgcggcttgg cgccgagatc ccggtcagca tacagattca gaacgtcacg
     3541 gatctgttct cggctcctgt acggctgcgg tttgatccca agcgccttcg tctgaatgaa
     3601 gtgactcgcg gcacgctgct atccggagac ggccagaacg taattttcac gagaaacatc
     3661 caaaacgatg tgggagaact gacggtcaac ctgaaccggc tgcccgggtc cgccggcgtg
     3721 agcggttccg gcactctgct gaacctgacg ttccaggcga tcggcgtggg gcaggcacaa
     3781 gtaaccgtgg tcgatggcgg attgcggaat tcccagatgc tgtcgattca ggccggcaat
     3841 cccatccttg taatcaatgt gaagtaggca atgagtatga ggcgatggcg gcaacggggg
     3901 ctcaccctgg tggaattgat tgtggcattc accattctgc tcaccctgac cagcatggcg
     3961 gtgccgatgg cccgcatgaa agtgaagcgc gagcaggagc gcgaactgcg gatggcgctg
     4021 cgcgagatcc gcgccgcgat cgacaagtat aaggatgccg cggacctgca acagatcggg
     4081 ccgaccaaga tggggtctga gaattatccg gaaagtctgg aaattctggt agagggcgta
     4141 tcgaaaacgg gagcggtgga tacgaaggtg aagttcctgc gccggatccc caaggatccc
     4201 atgacgaact cctacgactg ggggctacgc agcgttcagg acgatccgga gtcgatcgca
     4261 tggggaggcc agaacgtctt cgacgtatat acgaagagtc tcgacaaggc ggcggatggt
     4321 acaccctatt cgaagtggta ggcgcggatt tacgatcgtc gagctgatga tcgtgctgac
     4381 catcgtcggc attctggtct cgatcgcgat tccgatctat cagaagtcaa tcattcgggc
     4441 gaaggaaagt gtcctgaaga gcaatctttt cacacttcga acggtgatcg acgagtacac
     4501 ctacgacaaa cagaaggcgc cgcagtcgtt acaggacctc gtaaacgacg gctacctgcg
     4561 gcaggtgcct gtggacccga tgaccggctc ggattcaaca tggaagatca tcatggaaga
     4621 tgcgatgaac agcatcgacc agaccgaacc gggcattttt gatgtaaaga gcgggtcgga
     4681 caggatgagc ctggaaggaa cgccatactc cgagtggtga cgaggctgca gacgaagcgg
     4741 cccgcacacg gatgctgtta tcctatacag tgaatcacgg tgaggtgcga gagtggttga
     4801 atcgggcggt ctcgaaaacc gttgaacctt cacgggttcc gtgggttcga atcccaccct
     4861 caccgccagc atagaatcat aacgtagcgg caaagagccc aactcggtgg gtaatgatgt
     4921 taaacgggct ctgtgttctt gtggtcagtc atgagtgtga gaacccgact gcggcttgcc
     4981 cttcgatttc tttgatcggc ggcggccacc ctgcttctcc tcgaagacct ctcgatacag
     5041 ttccttcaac tgcgcccgga tcagatccag agcgtctgtt ccgagttttg tgagcctgta
     5101 ctctctgcgg ctgtggctgt tcacaggtgt ggcccggatg ggcttcagcc aaccatggct
     5161 ctccattcgc cggagtaagg gatacagcgt ccctgggctg atgtcatagc catgccgtct
     5221 gagttctccg atcatccatt gcccatacac ggtgccttcc gctgcatggt gcaggatgtg
     5281 gattttccag aagctcagca gcacttcgcg gatgagttgt gaatccttga gatccaattg
     5341 ccgattgtcc ttgagactcg ctgaactgtc aatacctcgc tgcgcagtaa ccttgacgag
     5401 cgatttcccg ccttggtata aacgagaatc tacaacgaca gacgtagacg aatatcgtaa
     5461 tatgccaatt caagagagcg accgcgtgca acggaacctg agcccggcat tcgtggacca
     5521 cgggtttacc gcgcgactcc gcggggaaga ggggccctga aaacggcgaa gaccaacgtg
     5581 caggaattgc aggggaattg ctgaaagaga gctgagatcc ggctgatgcg atttactata
     5641 atcactcgaa cactaggggt cggcgttctt cttgcctcga ttgggcccgg tacattgttg
     5701 ggccagtcat acaccatagg ccaagcagtg gcggatgctg gtgatcgcta tccggcgacg
     5761 agagtatcga tcgaacaagt gtccgccgcc gcccaagcaa tcaacctggc gcgaacggca
     5821 taccttcctc gcgtagactt cctaagccag gtaaaccgag ctacgcggaa caacgtgttc
     5881 ggaatgactc tgccgcaagc gacgcttcct gcgatctccg gaccaccgct ccctgagaat
     5941 gacatgacga gcgtgtttgg cagcactgtg ggctttaccg ttacgtggga gcccttcgat
     6001 ttcggtgccc gtcaggcaaa cgtccgctat gcggaagcag gccgtcgcgt ggcggaaaca
     6061 ggtgtttccc ggacccgttt cgaagtcacc acggctgccg cagatgctgt cctcaccgtc
     6121 atcgccgcgg aacaaaccgc tatcgcagca caggccggtg tcgaacgctc ccgagtactg
     6181 ctggaaatgg tgcaggcgca agttcgagcg cagcttcgtc ccggcgccga tgcaagccgt
     6241 gcccgtgcgg agcttgccct cgctcaaaac cagttgatcc gtgcacaaag cgcgatcgag
     6301 gttgcgaagg caaatcttac tcagttcgtt ggcggtgacc ccaccacgat ctccgttcaa
     6361 acggaaccct tgctcaagct accggatcca agcgcaggag cagggtcgtt gatgcctaac
     6421 catccggtac ttcaacaaca gcgctcactt atcgagcaga tccaagctaa cgagcaggtt
     6481 ttgcagcgct tgtattatcc acgtttttcg ctgcaaggct cgaactacgc gagggggacc
     6541 ggcgcgtatg caaacggctc gaccctgggt ggtctcaatg ggctcggtcc gaacatttac
     6601 aactggggcg tcggattgag tgtgacgttt cccttgttcg acctttcgtc gattcgttca
     6661 aagcgggaga tcgagcggta tcgggaacgc gcggagtcgg cacggttcga tcagtaccag
     6721 caagacttgg ccgccgccgt ctcgagcgcc cgagcgaatc tggacggcgc gcggagagtc
     6781 gcccaaaaca caccgattca actggacgcc gcacgggcca ctgagcagca gatgagtgcc
     6841 cgatataagt ccggattggg cacactgatc gaattggccg aggcacagcg ccttgtcacg
     6901 caggctgaga tcgacgatgc cctggcaagg cttggcgttt ggcgagctct gcttcaggcc
     6961 gccgtggcgc aagggaatct ggagccgttc ctgcaggaag cggggaagta ggggggctta
     7021 tgtggttagt caatgcagca atgagacgcc ccatcacaat tctggtcgtc gtgatggctg
     7081 ttgcacttgc gtccattctc gccatcaggc gtatgaaaat ggacatcttc ccgagtctcg
     7141 gctcgccgac gatctatgtc gcccagccgt acggcggcat ggatccatct cagatggaag
     7201 gattcctgac gtactactac gagtatcact tcctctacat cactggcatt gagcacgtgg
     7261 agtcaaagag cattcagggt gcggcgctta tgaagctcgt attccatccc gatacgaaca
     7321 tgaatcaggc ggttgcggaa acggtcggat acgtaaaccg gtcccgggct ttcatgcctc
     7381 ccggcgcggt tccaccgttc atcgtacgat tcgacgccgg cagtgtccct attgcgcaac
     7441 tcgtcttttc cagcccaaac cggagcccgg gcgagatgca ggactttgcc atcaaccgag
     7501 tgcgtcctct attcgccacg cttcccgggg tttcggcccc tcctcccttt ggtggtaatc
     7561 aacggaccat cattgttcgc atcgatcccg accgcatgcg cgcctatcag atgtctcccg
     7621 aagaggtcat tgcggcggtc aacaaagcga gtgcggtcct gccgtccggc ctcgtgcgca
     7681 tcggcaatga tgcgtatatc gcatccacta atgcaacggt cggcggcaat ctcgatgaat
     7741 tcatgaacac gccggttcga actggcgccg gcccgactgt ctacattcgc gacgtcggac
     7801 tggtcgaaaa cggtacggac atcttgagca gctacgcgca cgtcaacggc cgccggactg
     7861 tctatatccc ggtcaccaag cgctccgatg cgtccacgct cgacgtgatc cagcgtgtgc
     7921 gggaagcgct acccagaatg caggcggccg tgcccgaaga tgttgaggtc cggctggagt
     7981 tcgaccagac cggctacgta tcccgtgcgc tgaactcggt cgtttacgaa gcgctcatcg
     8041 gtgcggccct gacaggcttg atggttcttc ttttcctgcg ggactggcgc agctccctgg
     8101 tggtggtcgc caccattccc ttcgcgttgc ttgctgcggt agtgtgcctg tgggcggccg
     8161 gacagaccat taacatcatg acgctggcag gcctggccct ggcggtcggc atcctcgtgg
     8221 acgaagcgac ggtgctgatc gagagtatcc actcacacat gacgcaaggc gattcgaagg
     8281 tttgggctgt aatgggtgct tccgccaaga ccgctatccc tcgtctgctc gcgatgcttt
     8341 gcatcttggc tgtgttcgtg ccgtctttct tcatggctgg cgtcggacag caactgttca
     8401 tcccgctatc gcttgctgtc ggctttgcga tgatctcgtc atacatcctg tcgagttcgc
     8461 tggtaccggt gctatcgacg tggattttgc gcagagcgca ctgcgaggcc gaaacgggtc
     8521 tgttccattg gctgcgggaa atttacacga aatacttgcg attcacgctg aagctccgct
     8581 ggcctgttgc cgcggcgtac gtgttcggtt gcttcggact ggcgtggctg ttgctgccga
     8641 agatcggaac ggagatcttc ccgactgtcg atgccggaca gttccaattg cggctgcaag
     8701 ctcccaccgg cacacgcatc gagcaaaccg agcgtatcac acttcaagcc atggacttga
     8761 tcggcgaaac tttggggcgg gacaatgtgc ggatcacaac gagctttatc ggcgttcaac
     8821 cagccagcta tccgatcaat accatttacc tctggacgag cggaccacac gaggccgtat
     8881 tgctcgttgc actcaaaccg gaagccaagc ttcgtggaga agaactgaaa gaagagttgc
     8941 gggcgcgctt tcgaaagcgc atgcccgacg tgaggatttc gttcgaggcc ggcgatatag
     9001 tcgcacgcgt aatgagcttc ggctcgaata ctccgatcga ggtcgcagtg caaggtccga
     9061 acatcgcgca gaaccggaac cacgcggaga aagtgcggac ggaactcgcc aagctcccgt
     9121 tcctgcgtga tcttcagtat gcccagccgc ttgactaccc gactctccaa gtggacatcg
     9181 accgggaccg cgcgggccaa ttcgggatga ctgcggccgg ggtggcacgg tcgctcgttg
     9241 ccgccacttc gtcgagccgg ttcgtcgatc caaacttctg gcgagatccg aacagcggca
     9301 acgccttcca gattcaggta gaggtccctc agcacgagat ggcatcaatt gaggacatgc
     9361 gggcgctccc gatcacttcc aacggatcac gcgcgctgct tggagacgtc gccaatatca
     9421 aggtcacaag gtccctgggg ctcgttgagc gttacaacat gcagcgcgtg gttagtctca
     9481 ccgccaacat ctctggcaag gcactcggcc aggtcgtggc cgacatacag agagctaccg
     9541 cccgcgcagg ggacgcgccg agagggtcaa ccgtggcagt tcgcgggcag atccccgcgc
     9601 ttaacgagac catatccggg ctacaggtcg gactggggct cgccatcgcg gtcatcttcc
     9661 tgctgttggc ggctaacttc cagtcattca ggctggcgat cgctgtgatc acaacgattc
     9721 ccgcggtgct cgtcggagtt cttctcatgc tcctgattac cggcagcacg ctgaatgtgc
     9781 agtcgtttat gggagcgatc atgtcaagtg gcatcgcggt cgcgaactcc attctgctcg
     9841 tgacgtttgc cgaattcagc cggcatgccg gatcgaacgc cgttgatgca ggaatcgagg
     9901 gcggtcgcgg cagattgcga gcgattctga tgacggcaac ggcgatgatt gccggcatga
     9961 tcccgatcgc acttggaatc ggagaaggct ccgagcagac aacaccgctt ggccgcgccg
    10021 tgatcggcgg cctgttgttt gcgacttttg cgacgctgac ggtcttgccc gcgttctacg
    10081 ccatcctcca gcgcaaggcg acaccagtca cttcatcact tcatccgctg gacgcggagg
    10141 ggaggttcta tgaacccagg taagttcttt gtcgcactgg ccgtcacagg cgtattgtcc
    10201 gcacagaacg tgccggtggt gaaagtcgag tcaaaggcac tgagcagaac ggttctgctg
    10261 accggtgagt ttctgccgtt tcaaaccgtg gatctgcatg caagagttca aggtttcgtc
    10321 gagaaggtcc tcgtggaccg cggcagcatt gtcaaagaag gcgaattgct cgtgacgctg
    10381 agcgcgcccg agatggaagc gcaggttgcc gaggcggaag ccaaggtgaa gaccgccgaa
    10441 tcatcagcat cggaggcacg ggcgaagctt gtcgccgctg agagtacata caagcggctg
    10501 aaagaggcgt ccgcaaccga aggagcgatc gctggaaatg agttggtgat tgcggaacaa
    10561 tccgcggagg cgttacgaga tactgtccgc gcttccgaat ccgcgattac cgcagccaag
    10621 gcttctctcg cggccgtcca gaagatgcag cagtatctca gtatcagtgc gcccttcccc
    10681 ggcgtgataa cggagcgtct cgcacatccg ggcgcgctgg cgggacccag caccggccct
    10741 ctcctgcatc tggaacaagt ctcgcgtctt cggcttgtag tggccgtccc ggaagccaac
    10801 tttgcgggag tgcgcccggg aacaaaggtc cctttccagg tgtccgcata tccaacacgc
    10861 atgtttgagg gcgccgtcgc ccgtattgcg cgttcgatag atcaaaaaac gcgcacaatg
    10921 tccgttgagt tggaggtgcc caacgcttct ggcacgcttg cgcctggcat gtatccgcgg
    10981 gtgaactggc ccgttcgctc caccgagcga gcgttagtag tcccctcaac cagtgtcgtc
    11041 cggacaacgg agcgagtttt tgtgattcgc gtcaaggatg gcagagccga gtgggttgac
    11101 gttcaagtag gcgccagaga gtccgggcaa tttcaggtgt tcggcagcct cgccgagggc
    11161 gacatggttg tgcggaacgg cagcgacgag attcgtgacg gctcccaggt aagtgtgaca
    11221 acggcgaagc cagggtaggg cctggaactg cccttggggc cggcgggtgg agggtatgcc
    11281 cgatgtacga aattctgatg ggtgcgacat cggttatcgc cggagcgatc gcctccattg
    11341 tgggttttgg catcggaagc atcctgaccc cgttgctgtc tctgcggatt ggcacgcgcc
    11401 ttgcggtcgc cgtgatctcg atcccccatc ttttcgcaac gttcgtgcgc tttgtcgcaa
    11461 cggcaacagc catcggcttg gtggtcgatg ccgtgcgcat gcctgtgtac ttcgtgactg
    11521 caggcgatga agttctgaga atgccaggtg tgatcatcag cagcacggta gggggtgacc
    11581 gccacagcga acctgattcg caacttcgtc gagaattacc atgagaaact cgaggaagag
    11641 tacccctttc cgcgctt
//
LOCUS       DASTMK010000398         5427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_562958, whole genome shotgun sequence.
ACCESSION   DASTMK010000398 DASTMK010000000
VERSION     DASTMK010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562958"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..816
                     /locus_tag="VFQ79_17205"
     CDS             67..816
                     /locus_tag="VFQ79_17205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0141464.1"
                     /translation="MTNSQAAIRLENISKSFGSRTVLRDVSFDVDKGSVFCILGRSGT
                     GKSVTLKLIVGLLHADDGQIYIGDHNITGLNSRALVSVRKSIGFLFQNAALFDSISVG
                     ENVAFPLRRHTDLPEDEIHGRAQSKLEQVGLGKEYDKMPADLSGGMKKRAGLARALAL
                     DPAIVLVDEPSAGLDPITSSEIDELLVNLKSSRDTTLVVVTHNIPSARRIADELIMLH
                     EGGIIARGSVEEMEQTQDPIVQRFMASEDAG"
     gene            821..2275
                     /locus_tag="VFQ79_17210"
     CDS             821..2275
                     /locus_tag="VFQ79_17210"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0141465.1"
                     /translation="MSKRGNVLVGIFLLGGVLLFALGLFLIGNRRLLFEDSFHIHTEF
                     AKLAGLANGAKVRVGGMDAGEVVSVNVPAGPGSKFRVRARITEKLHPLVRKDSVATIQ
                     TDGIVGNQFLQIGPGTEQAAPAVAEGTIPSREPFDFADLLQELQTTVKIANATILDLR
                     GEVETVLHTINETAGEATDLIKDAGKDVKEIASSGAGISRDLGAIITDIRAGRGTVGK
                     LVTDERLYSQIKGVANDLSDSSTNVRNATADIRKLTTDLRERQVMEEVEKTLKNVRQI
                     SDQVKSTLAKFQQGEGTADLTTDLRQTLAYAREAMQDMAENSEALKRNWFFRGFFRDR
                     GFYDLDSVALRDYTGGQFARGKQVRREWVYYSSLFTPSKSGGFEISAEGKLKLDEVMG
                     EFFRYGRDSFLIVEGYASEGTGSEQFLNSRRRARAVRDYLVSTYHINPSSAGTMPMGA
                     VESSVNGKYWDGVAIALFYTGEPVPEKSSATTAP"
     gene            2549..5290
                     /locus_tag="VFQ79_17215"
     CDS             2549..5290
                     /locus_tag="VFQ79_17215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141466.1"
                     /translation="MRLFIALFAVAALSPAAEFTTGQAARALLGQHNFTTQDSSRAEN
                     SVGAAGGVAYANGQLFVTGSNRIVGIDPQLSRVVVFQDVNADVRTPDAEISVGSIRCP
                     VCSGSADVVLGQPDFTTNDWSNGPGGMRSPTAVASDGHYLAVADTDNNRVLIWNSIPS
                     SNQAPANVVVGQEDFSKTLSLPQPTASTLRGPQGVWIQDGKLYVADTLNHRVLIWNSI
                     PTSNGQAADVVLGQPNFNVQVQSNLAQAAIEPKPETMLNPVSVTSDGVRLFVTDLGHN
                     RVLVWNALPSSNGAPADFAVGQPDLNSALANNATALCQPVGQNDDGEDLYPFRCGATL
                     EFPRFALSDGTRLFVADGGNDRVLVFNTMPRDNGATADVALGQPSLLLNQVSDGSNPM
                     FRSAADQLRTPMALAWDGTNLYVTDPFNRRVMVFSMGEPAVPLTGVRNAASLDVAAVG
                     SIVFSGSVEENDEVTINIQDREYKYKVLADDSAVDVINALVNLINAGTGDPEVLATPN
                     TEIAAIILTSRLLGVEGNSITYSASASATSQIIVETSGATLAGGGDAARIAPGTIVTI
                     QGDGLAETTAEAPAEDWVLPTELGGVQVYFDGLKAPLLMVSPTKINVQVPWEILDATS
                     INAYVRTVKEGGNVVVTTPVAVPIVPFNPGIFAGDGPDPRPAMAVHASSQASGTVSVD
                     GSAKAGDVATVIIRAPEGAEEEDRRYTYTVQEGDTLTTVRDKLIEAINEDPRVFAFAA
                     GVFNRIRLRAREEGEGGNGIVYDVEVNDGAQVILTATTSALCCANEAGAPITEENPAL
                     PGETIIVYATGLGLVGPEEAQQSLWTGHKYDGPDVNEPYQFVDAMAGGKTANVLFAGL
                     KKGEHALYEVHLELNSDIPTNAATHVTIAQDVFVSNIVSIPVFNPNPPEEEEGETSP"
BASE COUNT         1125 a         1541 c         1646 g         1115 t
ORIGIN      
        1 tattttgatc gtgacgaacg tgatcctggt aaaagcgatt ttcttcgtct ttccggaggc
       61 gagcttatga cgaactctca ggccgcgatc cgcctggaaa atatctcgaa atcgttcggc
      121 tcgcgaacgg tgctgcgcga cgtctccttc gatgtggata aaggcagcgt gttttgcatt
      181 ctgggacgca gcggcacggg aaagagcgtg acgctcaagc tgatcgtcgg tctgctgcac
      241 gccgacgacg gacagatcta catcggggat cacaacatca ctggcttaaa cagccgggcg
      301 ctggtgtcgg tacggaaatc catcgggttt cttttccaga atgccgcctt gtttgattcg
      361 atctccgttg gtgagaacgt cgcgtttcct ttgcggcggc atacggatct tccggaagac
      421 gagattcacg gcagggcaca gagtaaactc gagcaggtcg gacttggcaa ggaatacgac
      481 aaaatgccgg ccgatctttc gggtggtatg aaaaagcggg ctggactggc acgcgcgctc
      541 gcgctagatc ccgctattgt gctggtcgac gagccgagcg ccggactgga ccccatcacc
      601 tcctcggaaa tcgacgagct tttggtgaat ctgaaatcca gccgggatac cactctcgtc
      661 gtcgtgacgc ataacattcc cagcgcacgg cgtattgccg acgagctcat catgttacac
      721 gagggcggga ttatcgcgcg cggatcggtg gaggaaatgg aacagacgca ggatcctatc
      781 gtgcagcggt tcatggcgtc ggaagacgcg ggttgattgt atgtctaaac gaggaaatgt
      841 tctggttggc atttttctgc ttggcggcgt gcttctcttc gcgctcggtc ttttcctgat
      901 cggcaaccgc cgcctgctgt ttgaagacag cttccatatc cacacggagt ttgccaagct
      961 ggcgggtctc gcaaacggag ccaaggttcg tgtgggagga atggatgccg gagaagtcgt
     1021 ttccgtgaac gttccagcgg gcccgggctc gaagttccgg gtgcgggcgc gaattacgga
     1081 gaagctccat cccctggtgc gtaaggattc cgtggcgacc atccagaccg acggcatcgt
     1141 cggcaatcag ttcctgcaaa tcggtccggg cacggaacaa gcggcgcctg ccgtcgccga
     1201 gggcacgatt cccagccgcg agccgttcga ttttgccgac ctgctgcagg agctgcaaac
     1261 aaccgtcaaa atcgccaacg cgaccattct ggacctgcga ggggaagtgg agactgttct
     1321 ccataccatc aatgagacgg ccggggaagc gacggacctc atcaaggacg caggcaagga
     1381 tgtgaaagaa atagccagtt cgggggctgg catctcccgc gacttgggcg cgatcatcac
     1441 cgatataagg gccggccgcg ggaccgtcgg caagctggtt accgacgagc gcctgtactc
     1501 tcaaatcaag ggagtggcga acgatttgtc ggactcctca acaaacgttc gcaacgcaac
     1561 cgccgatatc cggaaactca ccaccgatct tcgcgaacgg caggtgatgg aggaagtgga
     1621 aaagacgctc aagaatgtcc gccagatcag cgatcaggtg aagtcaaccc tcgcgaaatt
     1681 ccagcagggc gaagggaccg cggatctgac tacggatctc cgccagaccc tcgcctatgc
     1741 ccgcgaggcg atgcaggaca tggcggaaaa ctccgaagcg ctgaaacgca actggttctt
     1801 ccggggcttc ttccgggatc gcggcttcta cgatctcgat tcggtggctt tgcgcgatta
     1861 caccggcggg caatttgcac gcggcaaaca ggtccggcgc gaatgggttt actactccag
     1921 cctgttcaca ccttcgaaaa gcggaggctt cgaaatttcc gcggaaggca aactgaagct
     1981 cgacgaagtg atgggcgagt ttttccggta cggccgggac agctttctta tcgtcgaggg
     2041 ctacgccagc gaggggaccg ggtcggagca gtttctgaat tcacgccgca gggcacgggc
     2101 ggtgcgggac tacctggtaa gcacctacca catcaaccct tccagtgccg ggacgatgcc
     2161 gatgggagcg gtggagtcga gcgtaaacgg caaatactgg gatggcgtcg ctatcgcact
     2221 cttttacacc ggggaaccgg tgccggagaa atcctcagcc acaaccgctc cgtaagaagc
     2281 tgtgaagaaa ttgaattcag ccacggatga acacggatgc acacggataa gtttcctgtt
     2341 ttcaatctgt gtttatccgt gtccatctgt ggctctgaat ctttcacact tctaaccgtc
     2401 gcggctcaga attgcggagc cgcgcgcgca agcggtggtg acggcacact agtctgaaac
     2461 ccgttcccgg tcagctacgt cgcgtatcct tggtatactg ttgtatccaa cccgctcgcg
     2521 tccaaatgag gctccgtttc aggtacttat gaggttattc attgcattgt tcgccgtggc
     2581 ggccctgtcg ccggcggcgg agtttaccac cgggcaggcc gcccgcgcgc tgctcgggca
     2641 gcacaatttc acaacccagg attccagccg cgcggagaac agcgttggcg ctgccggcgg
     2701 cgtcgcatac gccaacggac aactcttcgt gacgggttcg aaccggattg ttggtatcga
     2761 tccgcaactc agccgcgtgg tcgtcttcca ggatgtcaac gccgatgtgc ggactccgga
     2821 tgccgagatt tcggtcggga gtatccgctg tccggtatgc tccggttcgg ccgatgtcgt
     2881 gctcgggcag cccgatttca caacgaacga ttggtcaaac ggtccgggcg gcatgcggtc
     2941 gcccacggcg gtggcttccg acggccatta cctcgccgtc gccgacaccg ataacaaccg
     3001 ggtattgatc tggaactcga ttccgtcgtc caatcaagcg ccggcgaatg tcgtggtggg
     3061 gcaggaggac ttttcgaaga cgctgtccct tccccagcca accgccagca cattgcgagg
     3121 accgcaaggc gtctggatcc aggatggcaa gctttatgtc gccgatacgc tgaatcatcg
     3181 cgtcctgatc tggaactcca tcccgacgag taatgggcag gctgcggatg tcgttctcgg
     3241 gcagccgaat ttcaacgttc aggtccagtc aaacctcgcc caggcggcaa tcgagcctaa
     3301 gccggaaacc atgctgaatc ccgtttcggt gacctccgac ggagttcgcc tgttcgttac
     3361 ggatcttggt cacaatcgcg ttctggtatg gaatgctctg ccatcttcaa atggcgcgcc
     3421 tgccgacttt gcggtaggcc aaccggacct taacagcgca ctggcgaaca atgcgaccgc
     3481 gctctgccag cctgtcggac agaatgacga cggtgaagac ctctatccgt tccggtgcgg
     3541 cgcgacgctg gagtttccgc gcttcgctct ctccgacggt acccggctct ttgtcgcgga
     3601 cggcggaaat gaccgcgtcc tggttttcaa caccatgccg cgggacaacg gtgctaccgc
     3661 ggatgtggcg ttggggcagc cttcgttatt gctgaatcag gtttccgacg ggtcgaaccc
     3721 tatgttccgc tccgccgccg atcaactgcg cacaccgatg gctttggctt gggacggcac
     3781 gaacctgtac gtcaccgatc ccttcaaccg ccgggtgatg gtgttctcca tgggtgagcc
     3841 ggctgtaccg ctcaccggcg tccgcaacgc cgccagtttg gatgtggcgg cagtcggtag
     3901 catcgtcttc agcggatcgg tggaggaaaa cgacgaggtc acaatcaaca ttcaagaccg
     3961 cgagtataag tacaaggtgc tggcggacga ttcggccgtc gacgtgatca atgcgctggt
     4021 gaacttgatc aatgcgggca ccggcgatcc ggaagtgctc gcgactccta ataccgaaat
     4081 tgccgcaatc attttgacct cccgccttct tggtgtagaa ggcaactcga ttacttattc
     4141 cgcttcggcg tccgctactt cgcagattat tgtcgaaacc agcggagcga cactcgccgg
     4201 gggcggagat gcagccagga tcgcccctgg caccatcgtc acgatacagg gggacggact
     4261 cgcggagacc acagcggaag cgccggcgga agactgggtg ctgcccaccg aactgggtgg
     4321 tgtccaggtg tattttgacg gcctcaaggc gccgttgctg atggtttcgc caacaaagat
     4381 caacgtacag gttccgtggg aaattctgga tgccaccagt atcaacgcat acgtccgcac
     4441 ggtgaaggaa ggcggcaacg ttgtggtgac gacgccggtc gcggtgccga tcgtcccgtt
     4501 caatcccggt attttcgccg gcgacggacc ggacccacgc ccggccatgg ccgtacacgc
     4561 cagcagccag gcgtcgggga ccgtttccgt tgacggctcc gcaaaagcgg gcgacgttgc
     4621 gacggtcatt attcgcgctc cggaaggcgc agaagaagaa gacaggcgtt acacctacac
     4681 cgttcaggaa ggcgacacgc tcaccactgt ccgggacaag ttgattgagg cgatcaacga
     4741 agatccacgt gtttttgcat tcgccgctgg agttttcaac agaatccggt tgcgcgcacg
     4801 ggaggagggt gaaggcggaa acgggattgt atatgacgtg gaggtgaacg acggcgccca
     4861 ggtgattctt acagcgacta cgagtgcgct gtgttgcgct aacgaagccg gtgctcccat
     4921 cacggaagag aacccggcgc tgcccggaga gacaatcatc gtttacgcga ccggactcgg
     4981 actagtgggg cccgaggaag cgcagcaatc gctctggacc gggcataagt acgacggccc
     5041 ggatgtcaac gagccgtacc agttcgtcga tgcgatggcg ggcggcaaaa cggctaacgt
     5101 tctgttcgcg ggcttgaaaa aaggcgaaca cgctctctac gaagtgcacc tggaactgaa
     5161 ctcggatatt ccgacgaatg cggctaccca cgtaaccatt gcgcaggatg tctttgtgag
     5221 taacatcgtg tcgattccgg tctttaatcc gaatccaccg gaggaagagg aaggggaaac
     5281 gtccccctag tgcatcgtcc cattagttct ttatagaatt cactttcttt cgcgtggccc
     5341 ttcggcggcg cggttttttg ggatgagcca cctgatagtc cttggtggtt tcgatgatgc
     5401 gctgcagttg ctcgggccct ttggtcc
//
LOCUS       DASTMK010000399         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_211320, whole genome shotgun sequence.
ACCESSION   DASTMK010000399 DASTMK010000000
VERSION     DASTMK010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211320"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VFQ79_17220"
     CDS             <1..1136
                     /locus_tag="VFQ79_17220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141467.1"
                     /translation="LAGAITFTRTLANLHDVDPGFRNKDVLTMSIELPVSSKTGESVG
                     RWSRVLAAVRETPGVSNAALCVFTPLSGRDSGATPVRIRGYQPANVEDSTVRVDHVSE
                     GYFETLGIRLLRGRLLSERDREGTLPVAVINESAARQFFGERDPIGQALEFPGKGAAN
                     VVYRIVGVVGDTKHNDLREASPRFAFIPIRQPLDAYRRLTLTVASAVPGGQAALLEPV
                     RSRLTAIDPGLLISEVITIRQQLDSTLLTERLLSGLSTSLGVLALILASVGLYGVMSY
                     RIGQQRQSIGIRMALGASPFSIASSVLRQSGGVIAAGLLCGLPFAFLAARMAGSLLWG
                     VESSDPTTYLMAAATLCLAGGVSAWIPARRASAIQPSEALRHN"
     gene            complement(1168..>3996)
                     /locus_tag="VFQ79_17225"
     CDS             complement(1168..>3996)
                     /locus_tag="VFQ79_17225"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEU0141468.1"
                     /translation="VANAQTPHQVDIVVLPDGGTPRDPRRIMRLGPREFHIQAVVEEG
                     KSPLTHALSRIDVIVHNPGSPADVQLHLDLSGNGTRINFDFSYYGGMPKRDFIYIQRP
                     GEPWRRIDGTTSGWVATVKFSVPNGDTKVGLGPWYPYADYLAFVSSLAPHPHLKKELI
                     GKSDGGREHWELTITDPSVPAQNKRTIFWHTREHAYETFSSFAMEGAVRQLLSEDAAE
                     MRRRFVFVLHPMTNVDGVALGYEYRGGYDFPDPHKTATGRLTFATVDRLRPHYCVAWH
                     NWTAPRDFCVAFYTDSENGKPSRRGWDLLTQRLPSPRAVGQRWDNDWVPNRHNWFNRK
                     TPNSNIHGYAANQYGSHIWGWEIPWWGRDEGDPAENSRQFGAAFARAFPATLFLLESN
                     AAPVRESPPVTVARWQIHEFELRGRAHVENLSRDTTIIGEFTSPSGKTLQVEGFYDGD
                     ETWRLRFAPTEEGEWTYLLRGEGVEIFQRGRIVAGPATTQGFIGVHPQNSYAFAYSDG
                     TAFFPMGDTCYGLFDDSPITPELRKKYLDTRRGQHFNFVRMSIGHSPYRASADPVYWA
                     WGGTAQQPDLDRLNPEFFKAFDRLLLEMRQAGMNAELLLFNYYRRPFTDPTQWTASRE
                     RLWLRNVMARYAAFDNVFLWTASNEYETYPDGRYRLDQPSDPDWAKSIGRAVKQLDPY
                     RHPYTVHPVVPSNNRGPSPRDPFEQPWRIGGFFGTGEEVDVLSQQTSTPYAGTWDETL
                     NAWTGDAAGVDASIAADRIYRKPVLNTENGYEYLKGYATNRRQVHHTGKVRRASWRIV
                     CAGGYFSAGFIGTIGHGDNWDRIDPSSKHPFVIKDEGAAGQLALLYEFFAELPFWRME
                     PCQPAVRGNGICFAAPGEVCVVYVPRGGRPIEVDAKLLKPGIAAQWFDPRTGKYSAAQ
                     DGSSSDGWLSFQVPDEDDWVLLIRSRRS"
BASE COUNT          778 a         1229 c         1210 g          779 t
ORIGIN      
        1 tgcttgcagg agcgatcact tttacccgga cgctcgcgaa cctgcacgac gtggatcccg
       61 gcttcagaaa caaagatgta ctcacgatgt cgatcgagct gccagtgtct tcgaagaccg
      121 gcgagtccgt aggacgctgg agtcgcgtgc tggctgccgt gcgggaaaca cccggggtga
      181 gcaatgccgc tctctgtgta ttcaccccct tgtcgggacg agacagcgga gcgacacccg
      241 tgcggattcg tggctatcag ccggctaacg tcgaagacag cacggttcga gtggatcacg
      301 tttccgaggg ctacttcgag actctgggga tccggttgct tcgcggccgc ctgctgtcgg
      361 aaagagaccg ggagggcaca ctgccggtcg cggtgatcaa cgaatcggcg gcgcgccagt
      421 tcttcggtga gcgggatccc atcgggcagg cgctcgagtt cccgggaaaa ggtgcggcga
      481 acgttgtgta ccgcatcgtt ggcgttgtcg gcgacacgaa gcacaacgat cttcgtgagg
      541 catctccgcg attcgccttt atcccgatcc ggcagccact ggacgcatac cgccggctta
      601 ccttgacggt cgcctctgcc gtgccaggcg gacaggcggc gcttctggaa ccggtccgaa
      661 gccggctcac cgccatcgat cccgggttat tgatttccga ggtgatcacg atccggcagc
      721 agcttgatag tacgctgctc actgaacggc tcctgtccgg actgtccacg agtttaggag
      781 tgctggcgtt gatcctcgca tcagtggggc tgtacggcgt aatgagctat cggattggcc
      841 agcagcggca atccatcggc atacgaatgg cgttgggagc atcgcctttc tccattgcct
      901 ccagcgtgct gcggcagagc ggcggggtca tagcggccgg gctcttatgc ggcctgccgt
      961 ttgcctttct cgcagcgcgg atggccggct ccttattgtg gggagtggaa tcgagcgatc
     1021 ccaccactta tctgatggca gccgcgacgt tatgcctggc tggcggcgtc agcgcctgga
     1081 taccggcacg acgcgcgtcg gccatacagc cttcagaagc gttgcggcac aactgaacgc
     1141 cactcaaccg cgccgcgcgg aagcattcta cgaacgtctc gagcggatca gcaaaaccca
     1201 gtcgtcttca tccggcacct gaaacgacag ccacccgtcg gacgaggagc cgtcttgcgc
     1261 tgcgctgtac ttgccggtgc gaggatcgaa ccattgcgca gcgatacctg gcttgaggag
     1321 ctttgcatcg acctcgatgg gccggccgcc gcgcggtaca tacacgacgc agacctcgcc
     1381 cggagccgcg aagcagatgc cattgccgcg caccgccggc tggcatggtt ccattcgcca
     1441 gaacggtaat tccgcaaaaa actcgtacag cagtgcaagc tggccggcag cgccttcgtc
     1501 cttaatcacg aacggatgtt tactggacgg gtcgatccgg tcccaattgt cgccgtggcc
     1561 gattgtgccg atgaatcccg cggagaaata tccgcccgcg caaacgatgc gccaggaagc
     1621 gcggcgcact ttgccggtgt gatgcacctg ccgccggttc gtagcgtaac ctttcaggta
     1681 ctcataaccg ttctcggtgt tgagcaccgg tttgcgatag atgcggtccg ccgcgatgct
     1741 ggcatcgacg ccggcggcat cgcccgtcca agcgttcagc gtctcgtccc acgttcccgc
     1801 gtacggcgtt gaggtttgct gcgaaagcac gtccacttcc tcgccggtcc cgaaaaaacc
     1861 accaatgcgc cacggctgct caaatgggtc gcgcgggctc ggtcccctgt tgttcgacgg
     1921 caccacggga tgcaccgtgt aggggtgacg gtacggatca agctgcttca ccgcgcggcc
     1981 gatcgatttc gcccaatccg ggtcgctggg ctgatccagc ctgtacctgc cgtcagggta
     2041 ggtctcgtat tcattcgacg cggtccagag gaagacgtta tcaaacgcgg cgtagcgcgc
     2101 catcacgttg cgcagccaca ggcgctcgcg cgacgcggtc cactgagtgg gatcggtaaa
     2161 tgggcggcgg tagtagttga acaacaacag ttcagcattc atacccgcct gcctcatctc
     2221 cagcagaagc cggtcgaacg ccttgaagaa ctcggggttc agacgatcga ggtccggctg
     2281 ttgcgccgta ccgccccacg cccaatacac gggatcggcc gaggcacgat acggactatg
     2341 cccgatactc atgcgcacaa agttgaagtg ttgcccgcgc cgggtgtcga gatacttctt
     2401 ccgtagctca ggcgtgatcg gactatcgtc aaacagcccg tagcaggtgt cgcccatcgg
     2461 gaagaaggcg gtcccgtcgg aataagcgaa ggcgtaagaa ttctgcggat gaacaccaat
     2521 gaaaccctgt gtggtggcgg gtccggcgac gatgcgcccg cgctgaaaaa tctcgacgcc
     2581 ctcaccgcgc aacaggtagg tccactcgcc ttcttccgta ggagcaaatc gcagacgcca
     2641 ggtttcatcg ccgtcgtaga agccttccac ctgaagcgtc tttcccgatg gcgatgtaaa
     2701 ctcaccgatg attgtggtgt cgcgcgacag gttttcaacg tgtgcccgtc cgcgcaactc
     2761 gaattcgtgt atctgccagc gcgcaacggt caccggaggg gactccctca cgggcgcagc
     2821 gttcgattca agcaggaaca gcgtcgcggg aaacgctctt gcaaaggccg ctccaaactg
     2881 acgggaattc tccgccggat cgccttcgtc acgtccccac cagggaattt cccatcccca
     2941 tatgtgcgaa ccatactgat tcgcagcgta tccatggatg ttagagtttg gagttttgcg
     3001 gttgaaccaa ttgtgacggt tcggcaccca gtcattgtcc caacgctgtc ctacagcccg
     3061 cggcgacgga aggcgctggg tgagcaaatc ccatccccgg cggcttggct tgccattctc
     3121 ggagtccgta taaaaggcga cacagaagtc gcggggcgcg gtccagttgt gccatgcgac
     3181 gcaatagtga ggccgcaggc gatcaacggt cgcaaacgtc agccggcctg tcgccgtctt
     3241 gtgcggatcg ggaaaatcgt acccgccgcg atattcataa ccaagggcga cgccgtcgac
     3301 attggtcatc ggatgcagaa caaaaacaaa cctgcgccgc atctccgccg catcctccga
     3361 caacaattgc ctgacggcgc cttccatcgc aaacgaggag aaggtctcgt aagcatgctc
     3421 gcgagtatgc cagaagatag tgcgtttatt ttgcgctggc accgacgggt ccgtaatggt
     3481 cagctcccaa tgctcgcggc cgccgtcgct cttgccgatc agttctttct tgagatgcgg
     3541 atgcggcgcg agcgacgaga cgaaggccag atagtcggcg tacggatacc acggtccgag
     3601 ccccaccttt gtgtcgccgt tgggcacgct gaacttgacc gtcgccaccc agcccgaggt
     3661 cgtgccgtcg atgcgccgcc acggttcccc cgggcgttgg atgtagatga aatcgcgctt
     3721 cggcattccg ccgtagtacg agaagtcgaa attgatccgg gtgccatttc ccgacagatc
     3781 cagatgaagc tgcacatccg ccgggctgcc ggggttgtga acgatcacgt cgatgcgcga
     3841 aagggcatga gtcagaggac tcttcccttc ctcaaccacc gcctgaatgt gaaactcacg
     3901 tggtccgaga cgcatgatgc gcctcggatc gcgtggcgtg cctccatccg gtagcacgac
     3961 aatgtcaacc tgatgaggtg tctgggcatt cgcgac
//
LOCUS       DASTMK010000400         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_147057, whole genome shotgun sequence.
ACCESSION   DASTMK010000400 DASTMK010000000
VERSION     DASTMK010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147057"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFQ79_17230"
     CDS             <1..883
                     /locus_tag="VFQ79_17230"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0141469.1"
                     /translation="RYQQRWYLVFREGTSRVSGDGIVRILSSDDGERWRSAGTLFYNG
                     GDLRDPKLAVSPSNTLMLSATAVIPSDAGVGHQTLIWYSGDGRDWSDPVQVGDPNLRL
                     WRVSWHDRRAFAVGYSTTAEKYLRLYTSKDGVFFKALADQMYSGDASESALFSGEEDS
                     LYCVSQHEGSSLLGISRPPYRGWTWRELDTRIAAPNILQLPDGRLVIAGRFHAEESRT
                     ALGWLDPKEGTVTRFLTLPSGGDNSSPGLVWYDGILWVSYHSSHEGKASIYLAKVKVP
                     TLETRKRTFDPNKPFEP"
     gene            883..1497
                     /locus_tag="VFQ79_17235"
     CDS             883..1497
                     /locus_tag="VFQ79_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141470.1"
                     /translation="MRILCLGLLILCAGCSREPQPAAGQKEEKVPAETKILHFYASPG
                     SLAPGETATICYGVENATAVEISPVVDELKPAFNRCFQVAPRQTTTYTLTATGAGTST
                     ASLVINVSGRAASAKERAPAQKSQMILFFLTSTPSVPAGLPATLCYGVTGAAKVSITP
                     PGPALELSERHCFTVKPTTTTTYTLTAVNNDGTRETQRLTVAVR"
     gene            complement(1501..1932)
                     /locus_tag="VFQ79_17240"
     CDS             complement(1501..1932)
                     /locus_tag="VFQ79_17240"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEU0141471.1"
                     /translation="MFDNCVEWFQLADELPGTQQTMIALVVFLLLTNVPQLTVVERER
                     AEKLESRLLAPCCYQETVSRHLSDAATAMRAEIAQFISDGKPDREILHIYKARYGARV
                     LVEPEGAAWWWMQTVPLVAAAAGLFFVVIVIRNWRRSALAT"
     gene            1984..2379
                     /locus_tag="VFQ79_17245"
     CDS             1984..2379
                     /locus_tag="VFQ79_17245"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEU0141472.1"
                     /translation="MPVLSGVPVGAELWATYESAFRDFAAKARIVQRYAADPLAARTD
                     VETAILDMAKARAAYTNARDAVARRLLDQTPENVLATRLEPSPEHTAELAELFWKFEG
                     KPEGTAEADWYRAEEILRSARKEWAHAAR"
     gene            complement(2389..3015)
                     /locus_tag="VFQ79_17250"
     CDS             complement(2389..3015)
                     /locus_tag="VFQ79_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141473.1"
                     /translation="MPVDPMQRLTQSGTPCSVQKRSGSVIKITSASMLVLLIGVFLSM
                     PQVLAAREREVRVPWEELAPLVEGRDVETVLTNGVYVRGRAQKVLHRELEVRVKRTSD
                     AAVIPKGETRIPRELLGALTVKSRKGPARVLLPPAMVFGVSLTLSRFTSIEDHVSSAV
                     WYPIAIGTGIAGYLLGRRLDTQSMRIIVTPTHSQRSEVAERSDYETPE"
     gene            complement(4014..>4215)
                     /locus_tag="VFQ79_17255"
     CDS             complement(4014..>4215)
                     /locus_tag="VFQ79_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU0141474.1"
                     /translation="IVDDSGKSALDMSRQGLRDILAAATERRMEVLIVEDCARLARDP
                     EDLQRILDSLAADGVIVVHGAE"
BASE COUNT          888 a         1246 c         1213 g          868 t
ORIGIN      
        1 gcggtaccag cagcgttggt atctggtttt tcgcgaggga acgtcgcgcg tctcggggga
       61 tggcatcgtc cggattttga gctcggacga tggcgagcgt tggcgttccg ccgggacgct
      121 gttttacaac ggtggcgacc ttcgcgaccc caaactcgcg gtttctccga gcaacacgct
      181 gatgctgagt gcgacggcgg tgatcccaag cgatgccggt gtcggacatc agaccctgat
      241 ctggtattca ggggacggcc gcgactggag cgaccctgtg caggtggggg acccgaatct
      301 gcggttatgg cgggtttcct ggcatgaccg ccgcgccttc gccgttggct atagcaccac
      361 ggccgaaaaa tatctgcgcc tgtacaccag caaggacgga gtttttttca aggcgctcgc
      421 ggatcagatg tatagcggcg atgcgagcga atcggccctg ttcagcggcg aggaggacag
      481 cctgtactgt gtgtcccagc acgagggttc ttccttgctc ggcataagcc ggccgccgta
      541 ccgcggctgg acatggcgcg aattggatac tcgcatcgcg gcgccgaaca tccttcaatt
      601 gccggatggc cgccttgtga ttgcgggccg cttccatgcg gaagagtccc ggactgcgct
      661 tgggtggctc gatccgaagg aaggcacggt gacccggttt ctgactctgc cctccggagg
      721 agacaacagt tctcccgggt tggtctggta cgacggaata ctttgggtaa gctaccactc
      781 atcacatgaa gggaaggcca gtatttatct cgcaaaagtg aaggtgccaa cgcttgaaac
      841 gcgcaagcgc accttcgatc cgaacaagcc ttttgaaccg tgatgcgaat tctctgcctt
      901 ggattgttga tcctttgcgc cgggtgttcc cgggaaccgc aaccagcggc cgggcaaaaa
      961 gaagaaaaag ttcccgccga aacgaaaatc cttcacttct acgccagccc gggttcgctg
     1021 gcacccggag aaaccgcaac catttgctac ggtgtggaga acgcgacagc cgtggaaatc
     1081 agtccggtgg tggacgagtt gaagcccgcc ttcaatcgct gcttccaggt cgcaccccgg
     1141 caaacgacca cttacacgct aaccgctacc ggcgcgggaa cctccacggc atcgctggtc
     1201 atcaacgtga gcgggcgtgc tgcttccgcg aaagagaggg cgcccgccca aaaatcgcag
     1261 atgattttgt tctttctcac cagtacgccg agcgtaccgg cgggccttcc agctactttg
     1321 tgctacggtg tgaccggcgc ggcgaaagtt agcattacac cgcccggccc cgcgctggag
     1381 ttgtccgaac ggcactgttt cactgtgaaa cccaccacga ccactaccta tacgctcact
     1441 gctgtcaata atgacggcac gcgcgaaaca caacgactca cggtggcagt ccgatgagat
     1501 tcaggtagcg agcgcgcttc gccgccagtt ccgtatcacg atcaccacga aaaacaatcc
     1561 cgcagcggcg gcaacgaggg gtaccgtctg catccaccac caggctgcac cttcgggctc
     1621 caccagcact ctggcgccgt accgtgcttt gtagatgtgg aggatttcac ggtccggctt
     1681 accatcgcta ataaactggg caatctcggc cctcatggct gtcgcggcgt cgctaagatg
     1741 ccgtgacaca gtttcctggt agcagcaggg cgccagcaga cggctttcca gcttctccgc
     1801 ccgttcccgt tcgacaacgg ttaattgcgg cacattggta agtagaagaa aaacaaccag
     1861 tgcaatcatg gtctgctggg ttcctggcag ttcatccgcg agttggaacc attcaacaca
     1921 attatcaaac aatccggtcc tccacttgaa cctaacgggc agctcgttcg tctaatactg
     1981 cgtatgccgg tattgagtgg agtgcctgta ggagcagagc tttgggcaac ctacgagagc
     2041 gctttccggg atttcgcggc aaaggctcga atcgtccagc gctacgccgc tgatccgttg
     2101 gctgcccgga ccgatgtgga aaccgccatt cttgacatgg cgaaggctcg cgctgcctac
     2161 acaaatgcac gcgatgcggt ggctcggcgg ctactggatc agacgccgga gaacgtgctt
     2221 gcgacaaggc ttgagccttc gccggaacac accgccgagt tggctgaact gttttggaaa
     2281 ttcgaaggaa aacccgaagg caccgccgag gcagactggt atcgcgccga ggagattctt
     2341 cgatctgccc ggaaagaatg ggcccatgcg gcacgctgat cctctcactt attccggcgt
     2401 ttcgtaatcg gatctctctg caacttcact gcgctgggag tgtgtgggcg taacgatgat
     2461 acgcatactt tgcgtgtcaa gccggcggcc aagcaggtat ccggctatcc cggttccgat
     2521 ggcaatcgga taccacacgg cactactcac gtggtcctct attgaagtga agcgcgacag
     2581 ggttagggaa acaccgaaca ccatcgcagg agggagcagc acacgcgctg gccccttccg
     2641 ggatttcacc gtcagggcac ctagcagttc gcggggaatc cgcgtctctc ctttgggaat
     2701 cacggcggca tcagaggtcc tcttcactcg gacctctaat tcccgatgca ataccttctg
     2761 cgcccggccc cgcacataaa ccccgttggt caacacggtc tccacatcac gcccctcgac
     2821 cagcggcgcc agttcctccc acgggacccg cacttcccgc tcgcgagcag ccagcacctg
     2881 gggcatagaa aggaagacac cgataagcag caccagcatg gaggctgacg tgattttgat
     2941 tacggaccca ctgcgcttct gtacgctaca tggcgtgccg gattgcgtca atctctgcat
     3001 cgggtcgacc ggcatcggtg atcccgcctc cgccgggcgg tggtgcacct gctttcagta
     3061 tgaagtccgg cgttaatgcc ggcaatagta ctctcgaagc cctccactgt atcgagcagg
     3121 cggcgaatct acgatataac atagcttgca aaaactgaga catgaataag ctattaacgc
     3181 ccaatgaaca actgcccggt ttggaaggag tcaccatcgg agatttctgg tcgtgggcgt
     3241 actcggacct tatgagcaac acagtccgtc cgcttttcgc cgagttcctt gttggcaggt
     3301 gtctcggctt aattgataag ccccgaaagg aatgggatca cgtcgacttt ctctatagcg
     3361 ggaaaaaggt tgaggtcaaa tcttctgcct acgtccaaac atggacccag accacggcgt
     3421 ctgtgatcat gtttgacatc gcagccaaag cgagaccgtg gctggcggaa acaaacagct
     3481 atggtccgtc gggcagctcc gccgactgct acgttctgtg cgttcacacc gacgtagacc
     3541 gcggatacag aggctctgct tgccggtcaa gtattccggg ctcaagcatg cgatcgatgt
     3601 agtacttttc ggagcttccg aggtcattcc cgcttccgaa tctacgccgt cgaagtgaca
     3661 ggactaagta gcaggctggt cagaggattg gtggacctgg agagattcga actctcgacc
     3721 tcttccatgc catggttgcg aaatcaatca cttacaaaga acggcgcgga aaataaaaga
     3781 gttacgcgtc ggtgctttgg acctcatttg gaccccatgc gctcttccat gcgtgtctgg
     3841 acctcaaaag gtccggcttc cgggattcgt ggttgcggcc cggacctcac gtggaatgcc
     3901 acttgcatcg cgcacgcgcg cggtcgaaac cgcccttccg attcttcgtg caggacaaag
     3961 aaaagggttt ccttatattg cggaagaccc cagaccgacg tggatgacgg ggttcactcg
     4021 gcaccgtgga ccacgatgac gccgtcggcg gcgagtgaat ccaggatgcg ctgaaggtcc
     4081 tcgggatcgc gtgccagccg ggcacagtct tcaacgatca gtacctccat ccggcgttcg
     4141 gtcgcggcag ccaggatgtc cctcagcccc tggcggctca tgtcgagggc agatttgccg
     4201 ctgtcgtcga cgatt
//
LOCUS       DASTMK010000401         9933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_466857, whole genome shotgun sequence.
ACCESSION   DASTMK010000401 DASTMK010000000
VERSION     DASTMK010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466857"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VFQ79_17260"
     CDS             <1..1292
                     /locus_tag="VFQ79_17260"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU0141475.1"
                     /translation="TNEIKAWIRRAAADSDVAIVEIGGTVGDIESLPFLEAIRQLRHE
                     LGRDDTMFAHVTLVPFIAAAQELKTKPTQHSVKELREIGIQPDLLFCRSERPLPHDVR
                     EKIALFCDVAPEAVITARDVKSVYEIPPIYAEQKVDEIILRMLRLPAGQLDLSRWMDM
                     LERMQNPDGEVSIALVGKYVEYEDSYKSLKEALLHGGLAHRLRVHIKWIEAEGLTWPD
                     CGRELANYDGILVPGGFGKRGIEGMINAIRYARENQVPYFGICLGMQTMVIEYARNVC
                     GLERANSTEFDPATPDRVIFKLRELKGVDELGGTMRLGAYPCELAENSFARQAYGAPV
                     ISERHRHRYEFNREYEQILTGHGLRITGHTPDGVYVEICELPSHPWYLGCQFHPEFKS
                     KPLRPHPLFRAFIGAAHRNRLLRLGTAAGSPQYSHAG"
     gene            1282..2154
                     /gene="kdsA"
                     /locus_tag="VFQ79_17265"
     CDS             1282..2154
                     /gene="kdsA"
                     /locus_tag="VFQ79_17265"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU0141476.1"
                     /translation="MQVEVPQPGGSPLFFSNGAPLALIAGPCVIESEEHVHFLAGEIL
                     RIAGAFVFKASFDKANRTGIGSPRGPGLQEGLRILAGLRSRGFAVLTDIHESWQAERA
                     AESADILQIPAFLCRQTDLLIAAGRTGRVVNIKKGQFVAPADIERAAEKVASTGNNSV
                     LLTERGTTFGYNNLVVDMRGLKMMAAGGRPVVFDATHSVQLPGASGDASGGQPQFIET
                     LSRAAVATGISGVFIEVHEAPERALSDGANALRLDLLGTLWARLQQLDALVKSDEFLN
                     GPDTSNVNRIIEEK"
     gene            2167..2625
                     /locus_tag="VFQ79_17270"
     CDS             2167..2625
                     /locus_tag="VFQ79_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU0141477.1"
                     /translation="MNRRRRGFSLIELLIVIAIILIIAAIAVPKLDKARMHSQETAAI
                     AQIRTIHTAQTQYFSQFGRYATTLTELGPPTSGQPGPAGADLIPGDLAMGSKTGYRFT
                     VQATPTGYAVTANPEVYNSTGRRNFYSDQSLVVKENWGSEPANPNSKEIK"
     gene            complement(2635..5070)
                     /locus_tag="VFQ79_17275"
     CDS             complement(2635..5070)
                     /locus_tag="VFQ79_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0141478.1"
                     /translation="MRWLVLALAVVFPAALAAAVEGGRTLETAASIRNSGLDPDACYR
                     VRDIALVREDLRVYLTEGFLIFGRPVNGRRYSAVFSAEVEGGDAELLLMPPHRSERMA
                     LAAFTGTPNLNERFRSAVFLFTDSTASELMDRIKARESELGRSAASPERGHILAANWD
                     PVVKNFSSSFEIRLVHDLLSPEREVAGFFYGAVSSIRLGNFDIIYDPHMRKQITAGQV
                     LHRDNQTFFDVWASFESRSVRSGARTISGSGFSVGQVRIDATIEPDLRLNATSRIAIK
                     PDRLLEGPLAFAISRQMRVSKARIDGQPAEVFQRESLRAGLLRGDHNMEFLIMGPEAF
                     QPGRSYEVEFRHEGEVVSSAGNGVFFVGSRGTWYPNDGTQSFSLYDLTFRYPKELDLV
                     ATGEVVEDRTEEQWRITRRTTSAPIRLAGFNLGNYEQSTATRGGYTVSVYANKQLESA
                     LQKSRDMILMPPPELTRTWPQRSRIPTLLSTVPDPRPPVQTRLHQLATNIASAFEFMA
                     ARFGPPPFSKLTVSPIPGMFGQGFPGLIYLSTLSYLEPKERPPAVRAQNVETFFSDII
                     DAHETAHQWWGNLVAAEEHQDVWIMEALANYSALLYLEKKNGTRGLESILNLYKANLL
                     APAPDGRPIDSAGPITWGTRLHSSQTPGAWKVITYEKGSWIMHMLRRRLGDEPFFAML
                     NEMAKRYRFRNITTEQFRQLAAEFAPKGAPDAKLEAFFEQWVYGTGIPALKLNYSAKG
                     KPPAVRISGTVAQSEVDPTFSVLVPVEIQFGKRNSIVRWVRTSEDPAEFSVTVPQPPV
                     RVVMDPSDSVLKR"
     gene            complement(5106..6899)
                     /locus_tag="VFQ79_17280"
     CDS             complement(5106..6899)
                     /locus_tag="VFQ79_17280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0141479.1"
                     /translation="MSALRPTPPGEAFYRTLFDNFVEPAFVVDGEGRSVDVNRRLCEL
                     LKMERQQLIGFPFTDFIPEEVRGRAVLAFEELRGKGDVHLDSPLRSADGSVLELEWNC
                     RADGASGLYLCSGRDVTERKNIEEERRRLAAIVESSDDAIISKTLEGIVTSWNRGAEK
                     VFGYTVEEMIGKPVSILAVPGRNEMPLILEKIRLGQPIEHYETQRRRKDGRLIVVSLS
                     VSPMRDSSGRITGASKIARDVTDRKLAEQALAEANQTMLDILESMDEGFIAFDGEGRC
                     TYLNGRVEEVTHKHRSELLGKVIWDVLPEMRGKKVFSEVERVRKERVTVTWEEYFAEH
                     DVWYQGHAYPTRDGVAMFIRNITERKRAEEILAQHAEEAARSSADLQQFAYATSHDLQ
                     EPLRNIVSFSQLLAHRYRDKLDSDADEFIGYIEDGARRMELLITDLVDYSRVASGRSG
                     ERLLVEMDSVIDWALRNLDSLIKDSGAVVSRDPLPSVKGDKIQLVQLIQRLVENAIRY
                     RGPEDPRIHIFSARADGEWWISVKDNGIGIEPQYSERIFGLFKRLNRNVPGTGVGLAI
                     CKRIAEKHGGRIWVESERGQGSTFRIALPAE"
     gene            7147..7626
                     /locus_tag="VFQ79_17285"
     CDS             7147..7626
                     /locus_tag="VFQ79_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141480.1"
                     /translation="MLSSKLIQLVEDHWEQITRRVIGEIRRDPELAHLGQLPDSELIE
                     WSGNIIRNLDLWLVSGRTKEIGDAYESLGRRRYQENVPLHELVRGTLTLKVVMVDFVR
                     SQGLHQTAVDIYAEEELDYLVGRFFDTVVYHGVKGYEQALRKESARPGVAGARTAGS"
     gene            complement(7623..8552)
                     /locus_tag="VFQ79_17290"
     CDS             complement(7623..8552)
                     /locus_tag="VFQ79_17290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0141481.1"
                     /translation="MAAESKAAIYGAIVANLGIAIAKFTAAAFTGSAAMVAEGVHSLV
                     DTGNGGLLLVGLHKSKAPADETHPFGYGKEIYFWSLIVAISIFAAGGGISIYEGILHV
                     FDPSPLQDPTWNYVVLGIAVVFESGALAVAYKQFRRATGGGPIWESVRRSKDPSTFAV
                     LFEDAAALLGLIAAFVGVYVGHLLEDSRFDGIASITIGLILVATAALLGRECKGLLIG
                     EGADPEELRAIRATLEADHAIDKVYKLLTMYFGPHTVLLTTEVEFRDGLSGQEVEETV
                     DRIEKAIRREHPHVHHIFIEAESIAETAKPGQS"
     gene            complement(8588..9757)
                     /locus_tag="VFQ79_17295"
     CDS             complement(8588..9757)
                     /locus_tag="VFQ79_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0141482.1"
                     /translation="MSIQPNVLQTLAPVQTIQAESLSDAPSPGIALCLSGGGYRAMLF
                     HLGTLWRLNELGYLPKLDRISSVSGGSITAGALGLLWSKLQFNAAGVAANFDETVAAR
                     IHGLAGHTVDTVSILSGVLGPGSVGDKVEQAYRRYVFGDATLQDLPDRPRFVINSTNV
                     QSMVLWRFSKPYMRDYRVGEVRNPKVPLAVAVAASSAFPPFLSPVVLKLEESDFTPAS
                     GEDLQRPPFTTEVVLTDGGVYDNLGLETAWKRYDTVLVSDAGGAYQPESDPKRDWAQH
                     SYRVFNLIDNQVRALRKRQVIASFKEGIRKGAYWGIRSNIADFRLPDALPCPFDKSTA
                     IAKTATRLKALEAPAQQRLINWGYAVCDAAMRKHVDPSLPAPAGFPYPAEGVGQV"
BASE COUNT         1982 a         3065 c         2909 g         1977 t
ORIGIN      
        1 tcaccaacga gattaaagcc tggatccgcc gcgccgccgc cgactccgac gtggccatcg
       61 tggaaattgg aggaactgtc ggcgatatcg aatccctgcc gttcctggaa gcgatccgcc
      121 agttgcgcca tgagctgggc cgcgacgata cgatgttcgc gcacgtgacg ctggtgcctt
      181 ttatcgccgc cgcgcaggaa ctgaaaacca aacctacaca gcatagcgtc aaggaattgc
      241 gtgaaatcgg tattcagccg gacctgcttt tctgccggtc cgagcgtccc ctgccgcacg
      301 acgtgcggga gaagatcgcg ctattctgcg acgtcgcccc ggaagccgtt atcaccgcgc
      361 gcgacgtcaa atcggtttac gaaatcccgc ccatttacgc cgagcagaag gtggatgaga
      421 tcattctccg gatgttgcgc ctgccggccg ggcagctcga cctcagccgg tggatggata
      481 tgcttgagcg catgcaaaat cctgatggcg aggtttccat cgcgctggtg ggcaagtatg
      541 tcgagtacga agattcatac aagagtctga aagaggcgct gctgcatggc gggctcgcgc
      601 accgcctgcg cgtccatatt aaatggattg aggcggaagg cctcacgtgg cccgactgtg
      661 gacgcgagtt ggcaaattac gacggcattc tcgtccccgg aggcttcggg aagcgcggca
      721 ttgaaggcat gatcaacgcc atccgctacg cgcgcgaaaa ccaggtaccg tatttcggca
      781 tctgcctcgg catgcagacg atggtgatcg aatatgcgcg caacgtctgc ggcctggagc
      841 gcgccaactc caccgaattc gatccggcca cccccgatcg cgtcatcttc aaactgcgcg
      901 agttgaaagg cgtggatgag ctcgggggca cgatgcgcct cggggcttat ccgtgtgagc
      961 ttgcggaaaa cagtttcgca cgccaggcct atggcgctcc ggtcatcagc gagcggcacc
     1021 gtcatcgtta cgagttcaat cgggaatacg agcagatact gaccgggcac ggtttgcgga
     1081 tcaccggcca cacgccggac ggcgtctatg tcgagatctg cgagctgccc agccatccgt
     1141 ggtatctcgg ctgccagttt caccccgagt tcaagtcaaa gccgttacgg cctcatccgc
     1201 tctttcgtgc gtttatcggg gccgctcatc gcaaccgcct actccggctc ggaaccgccg
     1261 ccggctcacc gcagtattcc catgcaggtt gaggttcctc agccgggcgg ttcacccctc
     1321 tttttctcga acggcgcacc gctggcgctg atcgccggac cctgcgtgat cgagagcgag
     1381 gaacacgtcc acttcctggc cggagagatt ctccgcatcg ccggcgcatt tgtttttaaa
     1441 gcgtcgttcg acaaggccaa ccggactggc atcggcagtc cacgggggcc aggtttgcag
     1501 gaaggcttgc gcatcctggc cgggctgcgt agccggggtt ttgccgtcct gaccgacatt
     1561 cacgaatcgt ggcaggcgga aagggcggct gagtctgccg acattctgca gattccggcg
     1621 tttctgtgca ggcagacgga cctgctgatt gcagccggac gcactgggcg ggtggtgaat
     1681 attaaaaagg ggcagttcgt cgcgccggcc gatatcgaac gtgccgcgga aaaagtagcc
     1741 tccacgggca acaacagcgt cttgctcact gaacgcggca ccacgttcgg atacaacaac
     1801 ctggtggtgg atatgcgcgg cctcaaaatg atggcggcgg gtggaaggcc ggttgtgttc
     1861 gacgcgacgc acagcgttca actgcccggg gcgtccggcg atgcttccgg cgggcagccg
     1921 cagttcatcg agaccctttc ccgcgcggcg gttgccaccg gcatcagcgg tgttttcatt
     1981 gaggtgcacg aagcgcccga acgggccctg tccgacggcg cgaacgcgtt gcgtctggac
     2041 cttctgggca cgctctgggc acgcttgcag caacttgacg cgctcgtgaa gtcggatgaa
     2101 tttctcaacg gaccggacac ttcaaacgtc aaccgtataa tagaagagaa gtaacaggag
     2161 agccgtatga atcgacgccg gcgcggtttt tcgctcattg agctgctgat tgtaattgcg
     2221 atcatcttga ttattgccgc gatcgcagtc cccaaactgg ataaagccag aatgcactcg
     2281 caggagaccg cggccatcgc gcaaatcagg accatccaca cggcccaaac gcagtatttc
     2341 tcccaatttg gacgatacgc cacaaccctg acggaactgg gcccccctac cagcggccaa
     2401 cccggccccg cgggtgcgga cctcattccc ggcgacctcg cgatgggcag caagaccgga
     2461 taccggttca ccgtccaggc gacacccacc ggctacgccg taacggcgaa tccggaggtc
     2521 tacaacagca ccgggcggcg taacttctat tccgatcaga gccttgtcgt caaggaaaat
     2581 tggggatcgg agccggccaa ccccaacagc aaggaaatca agtaaggcgc ggggctaccg
     2641 ctttaacacg ctgtccgacg ggtccatcac gacgcgaacc ggaggctgtg gcacggtgac
     2701 ggagaactcc gccggatctt cgctggtgcg aacccaccgg acgatggaat ttctcttccc
     2761 gaattgaatc tccaccggca ccagaacgct gaaggtgggg tccacctcgc tttgtgcaac
     2821 ggttccgctg atgcgtacgg ccggcggttt gccctttgcg gaatagttga gcttcagcgc
     2881 cggaattccc gtgccatata cccactgctc gaaaaaggct tccagcttcg catccggtgc
     2941 gcctttgggt gcaaattccg cggcaagctg ccggaactgt tctgtcgtga tgttgcgaaa
     3001 ccggtaccgc tttgccattt cgttcaacat cgcaaagaac ggttcgtcgc ccaaccgccg
     3061 gcgaagcatg tgcataatcc aggagccctt ctcataggtg atcaccttcc acgcgccggg
     3121 tgtttgcgac gaatgcagcc gcgtgcccca cgtaatgggt cccgccgaat cgatcgggcg
     3181 tccgtccggc gccggcgcca gcaggttggc cttgtataaa ttaaggatcg attcgagccc
     3241 gcgcgttccg ttctttttct cgaggtatag cagggcggag tagttggcga gagcctccat
     3301 aatccagaca tcctggtgct cctcggctgc caccagattt ccccaccact ggtgcgccgt
     3361 ttcgtgtgcg tcgatgatgt cggaaaaaaa ggtctccacg ttctgcgccc gcacggccgg
     3421 cggcctctct ttcggttcga gataagaaag cgtcgaaaga taaatcaggc ccgggaaacc
     3481 ctggccgaac atgcccggaa taggcgagac cgtgagtttg gaaaacggtg gcggaccgaa
     3541 ccgcgcggcc atgaactcga acgcggaagc gatgtttgtg gccagttgat ggagccgcgt
     3601 ttgcaccggc ggccggggat cggggaccgt cgacagcagg gttggaattc gggagcgttg
     3661 cggccaggtg cgcgtcaatt cgggaggcgg catcaggatc atgtcgcggc tcttctggag
     3721 tgcgctctcg agttgcttgt tcgcgtagac cgaaaccgtg tagccgcctc tggtggcggt
     3781 ggattgctca tagtttccga gattgaaccc ggccaggcgg atgggagcgg acgtggtccg
     3841 gcgggtaatg cgccattgtt cctcggtccg gtcttccacc acttcacccg tcgccaccag
     3901 gtccagttct ttgggatagc ggaacgtcag gtcgtacagc gagaacgatt gcgtgccgtc
     3961 gttggggtac catgttcccc gggatccgac gaagaatacg ccattgccgg cggacgacac
     4021 cacttcgccc tcgtgccgga actcaacctc ataagaacgg ccgggttgaa acgcttccgg
     4081 ccccatgatg agaaattcca tattatggtc gccgcgaagc agccccgccc gcagcgattc
     4141 gcgttgaaat acttccgccg gctggccgtc tatacgtgct ttggaaacgc gcatctgccg
     4201 ggagatagcg aacgccaggg gtccttccag gaggcggtcc ggcttgatgg cgatcctgga
     4261 tgtggcattc aggcgcaggt cgggctcgat cgtggcatcg atgcggacct gccccaccga
     4321 aaaccccgat cctgagatcg tacgcgcgcc gctccgcacc gatcgcgact cgaaactcgc
     4381 ccacacatcg aagaacgtct ggttgtcgcg atgcagcacc tgtccggccg tgatctgttt
     4441 gcgcatatgc gggtcgtaga tgatatcgaa gttgcccagt cgaatacttg acacggcgcc
     4501 gtagaaaaag cccgcgactt cccgctcggg cgacagcaaa tcatggacca ggcggatctc
     4561 gaagctgctg ctgaaattct tcaccaccgg gtcccagttc gccgccagga tatggcccct
     4621 ctccggggag gcggcgctgc ggcctagctc agactcacgg gccttgatgc gatccatcaa
     4681 ttccgatgcg gtggaatcgg tgaacaaaaa cacggcggag cggaaacgtt cgttcaggtt
     4741 cggcgtgccg gtaaacgccg ccagtgccat cctctcgctg cggtgcggtg gcatcaacag
     4801 cagctccgcg tccccgcctt ccacctcggc ggaaaacacc gcgctgtagc gccggccgtt
     4861 caccggcctg ccgaagatca ggaatccttc ggtgaggtat acgcgcagat cctcgcgcac
     4921 cagcgctatg tcacgaacgc ggtagcaggc atccggatcg agaccggagt tccggatgga
     4981 agccgcggtt tcaagcgtgc gccctccctc cacggcagcc gccaatgcag ccggaaacac
     5041 caccgcgagt gccagcacaa gccatctcac actcccagtc taccccgcgt attacgggtt
     5101 agatttcact cggcgggtaa agcgattcga aatgtggaac cttgtccccg ttcggattcc
     5161 acccagatgc ggccgccatg cttctcggcg atacgcttgc aaatggcgag cccgacaccg
     5221 gtgccgggca cgttgcggtt gaggcgtttg aacagtccga agatccgttc ggaatattgc
     5281 ggttcgatgc cgatgccgtt gtccttcacg gagatccacc attctccgtc cgcgcgcgcc
     5341 gagaaaatat ggattcttgg gtcttccggc ccccggtacc ggatcgcgtt ttccaccagc
     5401 cgctgtatca attgcacaag ttgaatcttg tcgcctttga cggagggcaa cggatcgcgg
     5461 ctgacaacgg cgccgctgtc tttaatcaac gagtcgagat ttcgcagcgc ccaatcgatc
     5521 acggagtcca tctccacaag caggcgttcc ccgcttcggc cgctcgctac gcgggaatag
     5581 tccaccaggt cggtaatcag caactccatc cttctggctc cgtcctcgat gtaaccgatg
     5641 aactcatcgg catcggaatc cagcttgtcc cggtaccggt gagcaagcag ttgcgaaaaa
     5701 ctcacgatgt tgcgcagcgg ctcctgcaga tcgtgactgg tggcatacgc aaactgctgc
     5761 aaatcggcgc tggagcgggc cgcttcttcg gcgtgctgcg ccagaatctc ttcggcccgt
     5821 ttcctctccg tgatattccg gataaacatc gcaacaccgt cccgcgtggg ataggcgtgt
     5881 ccctgatacc agacatcatg ctccgcgaaa tactcctccc acgtgacggt cacgcgctcc
     5941 ttgcgcacgc gctccacttc gctgaaaact ttctttccgc gcatctccgg aagcacatcc
     6001 catatcactt tgcccagcaa ttcggatcgg tgcttgtgcg tgacttcctc taccctgccg
     6061 ttgaggtacg tacaccgtcc ttcgccatcg aaagctatga acccctcgtc catgctttca
     6121 agaatgtcga gcattgtctg gttcgcctcg gcgagcgcct gttccgcgag tttccggtcc
     6181 gtcacatcgc gggcgatctt cgaagcgccg gtgatgcggc cggacgaatc ccgcatgggc
     6241 gatacggaca gagaaaccac gatcaatcgc ccgtctttgc ggcggcgctg tgtttcatag
     6301 tgctcgatgg gttgtccgag ccggatcttc tcgaggatta gcggcatttc gttgcggcct
     6361 ggtaccgcca gaatcgagac cggcttcccg atcatctctt cgaccgtgta tccgaaaacc
     6421 ttttcagccc ccctgttcca gcttgtaaca attccttcca gagttttgct gatgatcgca
     6481 tcgtcggagg attccacaat ggcggccagc cggcggcgct cctcttcgat gttcttcctt
     6541 tccgttacgt ctcggccgga acaaagatac agccccgacg caccgtccgc gcggcaattc
     6601 cactccagtt ccaggacact gccatcggcg ctccgcagtg gagagtccaa atgaacgtcg
     6661 cccttgcccc ggagttcctc gaacgcgagg accgctctac cccgcacctc ctccggaatg
     6721 aaatccgtga aagggaaacc aatcaactgc tgccgttcca tctttaacaa ctcgcacagc
     6781 cggcggttca cgtcgacgct ccggccttcc ccgtcgacga caaaggcggg ctctacgaag
     6841 ttgtcgaata gagtacggta aaacgcttca cccggcggcg tcgggcgaag ggcggacaac
     6901 tctcctctgt cctttttttg tttccttccc ggcatgcgct cgtatgatgt ccgttacagc
     6961 gagataacgg tcttcccctt caccctttcc cctccccatc ttagctccgc ctgcgggatg
     7021 ccgtctccgc cacgacgatg gccggattga caccggcaca ctatgggtct actcatggag
     7081 tagcgagttt ccggtacatt tcgcaagagc ggttcctttc accggaaaac ttgggggagg
     7141 tgagtaatgc tctcgtcgaa actcatccaa ctggtcgaag atcattggga gcagattacc
     7201 cggcgggtga tcggcgaaat ccggcgcgac cctgaactcg cgcacttggg ccagcttccc
     7261 gactcggaat tgatcgagtg gagcggaaac atcatcagga acctcgacct ctggctggtc
     7321 agcggaagaa cgaaggaaat cggtgatgcc tacgaatcgt tggggcggcg ccgctaccag
     7381 gaaaatgtcc ccttgcacga gctggtccgt ggcacgctaa cgctgaaggt ggttatggtg
     7441 gatttcgtgc gcagccaggg tttgcaccag acggctgtcg atatctacgc cgaagaagag
     7501 ctcgactatc tcgtcggccg ttttttcgac acggtcgtgt accacggcgt gaagggctac
     7561 gagcaggcgc tgcggaagga aagcgcgcgg cccggagtgg cgggagcgcg aacggcaggc
     7621 agttagcttt gtcccggctt ggccgtttcg gcgatcgatt cggcttcgat gaagatgtga
     7681 tggacatgcg ggtgctcgcg ccggatcgct ttttctatgc gatcgacggt ctcctccact
     7741 tcctgtcccg aaaggccatc gcgaaactcg acctcggtgg ttagcaaaac ggtatgcggg
     7801 ccgaaataca tggtcagcaa tttgtaaact ttatcaatag cgtgatccgc ctcgagcgtt
     7861 gcacggatcg ctcgaagttc ttccggatcg gctccttcgc cgatcagcaa tcccttgcat
     7921 tcccgcccca acaacgcggc cgtagccacc agaatcaagc cgatggtgat ggaggctatt
     7981 ccatcgaaac gcgaatcctc cagcaggtgg cccacataaa cacccacgaa cgcggcgatc
     8041 aggcccaata gcgccgctgc atcctcgaac aacacggcga acgtcgatgg gtctttactc
     8101 cggcgaacac tctcccagat cgggccgccg cccgtggccc ggcggaactg cttataggcc
     8161 acggcgaggg cgccgctttc gaacaccacc gcgattccca ggacaacata attccaggtg
     8221 gggtcctgaa gcggagaagg atcgaatacg tgaagaattc cttcgtaaat cgagatgccg
     8281 ccgcccgccg cgaaaatgga aatggcgaca atcaggctcc agaaatagat ctctttcccg
     8341 tatccgaacg ggtgtgtctc atcggccggc gctttgctct tgtgaagccc gaccagcagc
     8401 agacctccgt tgccggtatc gacgagggag tgaacgcctt cggcgaccat ggccgcgctt
     8461 ccggtgaaag ccgcggcagt gaacttcgca atggcgatgc ccaggttcgc aacgatcgct
     8521 ccataaatag cggctttcga ttctgctgcc attcgcctcg cgcccccgat ccccttcccg
     8581 cggcttacta gacttgtccc acgccttccg ccggataggg aaagcccgcc ggtgccggaa
     8641 gcgacgggtc cacatgtttt cgcatagccg cgtcgcagac ggcatagccc cagttgatga
     8701 gcctctgttg cgccggcgct tccagcgcct tcagccgtgt cgcggttttg gcaatcgcgg
     8761 tcgatttgtc gaacgggcag ggcagcgcgt ccggcagccg gaaatcggca atattcgatc
     8821 gtatgcccca gtaagctccc ttgcgaatcc cttctttgaa agacgcgatg acctggcgtt
     8881 tgcgcagcgc gcgcacctga ttgtcgatga ggttgaaaac gcggtagctg tgctgcgccc
     8941 aatcgcgctt gggatcggat tccggctggt aggcgccccc cgcgtcgctc accagaactg
     9001 tgtcatacct cttccatgcg gtctccaggc cgaggttgtc gtagacgcct ccgtccgtca
     9061 gaaccacttc tgtcgtaaaa ggcgggcgtt gcaaatcttc accgctcgcg ggagtgaaat
     9121 cggactcctc gagcttcagc accaccggag acagaaatgg agggaacgcc gaggaggcgg
     9181 caaccgcgac cgccagcggc accttggggt ttcgcacttc gccgacccgg taatcccgca
     9241 tgtaaggttt cgaaaagcgc catagaacca tcgactgcac gttagtagag ttgatgacga
     9301 aacgcgggcg gtcgggaagg tcctgcagcg tggcatcgcc gaaaacgtag cggcgatagg
     9361 cctgctccac cttgtctcct accgagccgg ggccgagcac gccggagagg atggaaaccg
     9421 tatccaccgt gtgcccggcc agcccgtgaa tgcgggccgc cacggtttca tcgaagttcg
     9481 ctgccacgcc tgctgcatta aactgcaatt tcgaccagag caagccgaga gcgccggctg
     9541 tgatggagcc gccggaaaca ctggagatcc ggtcgagttt gggaagatat ccgagttcgt
     9601 tcagccgcca cagcgtgccc aggtgaaaca gcatcgcgcg gtaaccgcct ccggataaac
     9661 aaagcgcgat tccgggcgaa ggcgcgtcgg acagcgattc ggcctgaatg gtctgaacgg
     9721 gcgcgagcgt ctgcagaaca ttgggttgta tggacatgag gtggagatcc ttcgcgcatt
     9781 gtaaacccgc cgcctgccgt tacgatacgg gatggtgaaa aaatatccgc cccgcctacc
     9841 aaacccggca ggcaggctcg cggaccatgg gagcatcctg cttgcagccg aacgccttcc
     9901 ggaattcagg gctgttcgaa accacgccgt tga
//
LOCUS       DASTMK010000402         5348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_116428, whole genome shotgun sequence.
ACCESSION   DASTMK010000402 DASTMK010000000
VERSION     DASTMK010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116428"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFQ79_17300"
     CDS             <1..876
                     /locus_tag="VFQ79_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEU0141483.1"
                     /translation="RGGMAAKRGGTQAGQARRSAVRPDLATLGGLIVAFGGIVGGLIL
                     EGGAIGDIAQPTGAMIVLGGTLGAVMVTTPLNVLLRAIKRLKIVFLEEIHSPAVVIDE
                     IIEFATKARKNGIVSLEQDAESVQEPFLRKALNLAVDGTDLQEIRKMMELELTLEEQH
                     AEAEAKVFESAGGYSPTIGIIGAVLGLIQVMKHIDQPSEVGHGIGVAFVATVYGLLLA
                     NVFLLPMGNKIKARVHSAIQIKELMLEGVIGIVEGLNPKLIRSKLDAYVHQSRPSKKA
                     ARNAAVRNAEASAET"
     gene            888..1685
                     /locus_tag="VFQ79_17305"
     CDS             888..1685
                     /locus_tag="VFQ79_17305"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEU0141484.1"
                     /translation="MARQKKHAAHENHERWLVSYADFITLLFAFFVVMYASSQADKAK
                     AAEVAASVRTALEDGKASSVIAAIIGSQKELDKNTKPAKQAVAAPEDPEVVADLLPSL
                     LLLEKELAEEIKAGEMKVTMQPRGLVVTLREAAFFPSGQDTMVPNALPSIGKVAGVIQ
                     KIGNPVRLEGHTDSVPISNSRFRSNWELSAARAIAMLDLFTNRFSINHHRLAVAGYAD
                     IIPVESNDTAEGRARNRRVDIVILKGSSLENEPPPPQRIPKPSPTAP"
     gene            1739..3481
                     /locus_tag="VFQ79_17310"
     CDS             1739..3481
                     /locus_tag="VFQ79_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0141485.1"
                     /translation="MEQIGRYRVVGEVGRGAMGLVYRAEDPAIGRTVAIKTIRLSELS
                     DPNERERLRERFAREAQSTGILSHPNIVTIFDVVQEGDTTHLVMEFVSGATLERLMSS
                     EQIPNRGVLIDILAQTAGALDYAHRKGIVHRDIKPANIAVTEEGLVKITDFGVAKIVS
                     QQMTHAGVLMGTPNYMSPEQVQGLPVDGRSDQYSLAVIAFELLTGEKPFNADYLPTLL
                     FRIMTDAPPVPSHLNQTLGAPIDAALIKALAKQPEDRYATCCDFVTALKQACYSVPEW
                     EPVPRGSSLAIPTLVVPGGEVSGPRYKPDVASEPPPAAETARRDRLARYREAEPARET
                     RGRRTAWKSLAAVVSAVLLVAALFWGVRRFSPFAPPVSAPEEKIASDPKVPDTGSRPS
                     PTDEPAGQGSPAASPPPAQESEEQEGPEGEPAPAQEEPAVVRRPSSTAEYTTQISSSP
                     SGAAVVFDNNPSIHCRTPCAIPLLAGRHTLSAELEGYRQALRIFEVPAEREVFVPLER
                     RTGQLSIRSNPPGASISINGEPRPEKTPVTLSLPAGKYRVGVGGAGHPQEVTDVEIKD
                     GDLKNLVIDWSGKG"
     gene            3549..4844
                     /locus_tag="VFQ79_17315"
     CDS             3549..4844
                     /locus_tag="VFQ79_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU0141486.1"
                     /translation="MRMASLGLAVASATLAAAATPMKIRVEPVTDTVRAGESPSLVVQ
                     LMDADNRPAPAPKDLPVKISARLPSNAAEEVAGIIIRAGQSSATIKLPSPRATGPVYY
                     WARQQELITGGTFVMVESGQPPAAGDVPASPYVVTLRYSPQREFLADGKDPVTVHAFV
                     FSRNDTPLPAFRITLFDSSGTINPVPLVIPPGQGSGTATLTSKSPGEVKVEYVSSVPR
                     ANVDGDPVLKIPFGPPITALQLSVNPREISLVDRADLVVRLVDEHGTPLATTRPRTIS
                     LALDRGRGAIEKSELTIPPGSAEVRTGFSPLWWGTMGVAVSSPNLFTVTAPMEVKLPF
                     ALLGISLLGGLAGAYVFLLRNRRTKPWRVFVGALTGFVFYWACLFLSLVGLPGTIVLN
                     PLSAFVLSVLGGWMGPEIFERLLARFGFAKEKAKARGAA"
     gene            4935..>5348
                     /locus_tag="VFQ79_17320"
     CDS             4935..>5348
                     /locus_tag="VFQ79_17320"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU0141487.1"
                     /translation="MLCLCETGVLRSAEDPPAQPIPFSHKQHAAAGAACVDCHTTAKK
                     AARAGMPDTARCLLCHRSVKQDSPAIVKLRKMHEDKEPIEWIRLYQVPDFVFFSHEKH
                     LAAGAACSECHGPVEQREVLTKEVPTTMAACMNCHK"
BASE COUNT         1098 a         1575 c         1660 g         1015 t
ORIGIN      
        1 cgggggggca tggcagcgaa gagaggtgga acccaagccg gacaagcccg gcgatctgct
       61 gtcaggccgg atctggcaac gctgggtggc cttattgttg cttttggcgg gatcgtgggc
      121 ggcctcattc tcgaaggggg cgcaattggc gatatcgccc agccaaccgg ggcaatgatc
      181 gtgctcggag gcacccttgg cgccgtcatg gtgacgacgc cgttgaacgt gctgctgcgg
      241 gcgatcaaaa ggctgaagat tgtcttcctg gaagagatcc attccccggc cgtggtaatc
      301 gacgaaatca tcgaatttgc gacgaaagcc cgaaaaaacg gcatcgtttc gttggaacag
      361 gatgcggagt cagttcagga accctttctc cgcaaggccc tcaacctggc tgtagacggg
      421 accgacctcc aggaaatccg caaaatgatg gaactggagc ttactctcga agagcagcac
      481 gcggaagcgg aagcaaaagt gtttgaatct gccggaggat attcccccac cattggtatt
      541 attggcgccg tgctcggcct gattcaggtc atgaagcata tcgaccagcc ctcggaagtc
      601 ggtcacggca ttggagttgc tttcgtggct acagtttacg gattgctgct ggccaacgtg
      661 ttcctgttgc ccatgggcaa caagatcaaa gcgcgggtgc attcggcaat ccaaatcaag
      721 gaactgatgc ttgaaggcgt cattggcatc gtggagggcc tgaatccgaa gttgatccgc
      781 agcaaactgg acgcctacgt ccaccaaagc cggccttcga agaaggcggc gaggaacgcc
      841 gcggtgagaa atgcggaagc ttccgcggag acttgagaac tcacacaatg gcccggcaaa
      901 aaaaacatgc ggcgcatgaa aatcacgagc ggtggctcgt ttcgtatgcc gatttcatca
      961 ctcttctgtt cgcattcttc gtagtaatgt acgcttcttc gcaggccgac aaggccaaag
     1021 ccgcggaggt ggctgcgtcc gtccgcacgg cactcgagga cggcaaagcc tcctcggtca
     1081 tcgccgccat catcggttcc cagaaggaat tggacaagaa taccaagccg gccaaacagg
     1141 cggtcgcagc gccggaagat ccagaagtgg ttgccgatct gctgccttcc ctcctgttgc
     1201 tcgaaaagga gctcgcagag gaaatcaaag ccggagagat gaaggttacc atgcagcccc
     1261 ggggcctggt ggtcacactc cgggaagccg ctttctttcc atcgggccag gatacgatgg
     1321 ttccaaacgc gctccccagc ataggaaagg tcgccggcgt catccagaaa attggaaatc
     1381 ctgtccgtct ggaaggccac accgactcgg tgcccattag caactcgcgg tttcgcagca
     1441 actgggaact gtccgccgcg cgagccatcg cgatgctcga tttgttcacc aaccgcttca
     1501 gcatcaacca tcatcgcctg gcggtggcgg ggtatgccga cattatcccg gtggagtcca
     1561 acgacaccgc cgaggggcgt gcccgcaacc ggcgagtgga tattgtgata ctcaaaggct
     1621 ccagtctcga aaatgagccg ccacccccac aacgtatccc gaagccctca cccacggcac
     1681 cctgatcgtc tgggcggggg gcatgacgca cctgcggtaa aatgacaggc ggcgcggcat
     1741 ggagcagatt ggccggtaca gggtcgtcgg cgaggttggg cgcggcgcta tgggccttgt
     1801 gtaccgggct gaagatcccg cgatcggccg gaccgtcgcc attaagacca ttcgcctctc
     1861 ggagctgagc gacccgaacg aacgggagcg gctgcgggag aggtttgccc gcgaggcgca
     1921 gtcgaccggc atcctgtccc acccgaacat cgtcacgatt ttcgatgtcg tccaggaagg
     1981 cgataccact cacctggtga tggagtttgt gagcggagcg acgctcgagc ggttgatgag
     2041 ctccgaacaa attcccaacc gcggtgtgtt gatcgacatt ctggcgcaga cggcgggcgc
     2101 tctcgattat gcacaccgga aaggcattgt ccatcgcgac atcaagccgg cgaacatcgc
     2161 cgtcaccgaa gaaggactgg tcaagatcac cgatttcggg gtcgccaaga tcgtatcgca
     2221 gcagatgacc catgccggag tgttgatggg cacgccaaac tacatgtcac ctgaacaggt
     2281 tcagggactc ccggtggacg gaaggtcgga ccagtattcg cttgctgtga ttgcgtttga
     2341 gctgctcacc ggagaaaaac cgttcaacgc cgactacctg cccaccctgc tgttccggat
     2401 catgacggac gccccgcccg tcccctcaca tctgaatcaa acgctcggtg cgcccatcga
     2461 cgccgcgctg atcaaggcgc tggccaagca gccggaagac cgctacgcca catgttgtga
     2521 ttttgttacg gccctcaaac aggcttgcta ctccgtaccc gaatgggagc cggtccctcg
     2581 cggaagcagt ctcgcaattc caaccttggt agtgccgggc ggcgaagtgt ccggtcctcg
     2641 ctacaaaccg gacgtagcca gtgaaccgcc gccggccgcc gaaacagccc gtcgcgacag
     2701 gctggcgcgg tatcgggaag ccgaacctgc acgggagacg cgagggcgaa gaacagcctg
     2761 gaagagcctg gcggcggtgg tgtccgcggt gctgctcgtg gcggctctgt tttggggtgt
     2821 gcgccggttc tctccattcg ctccacccgt atccgcacca gaggagaaga ttgcatctga
     2881 ccccaaagtt cccgataccg gatcgcggcc gagccccacg gatgaaccgg cagggcaagg
     2941 ttctcccgca gcgagccctc cgcctgctca ggaaagtgaa gagcaggagg ggccggaggg
     3001 cgagccggcg ccggcgcaag aagaacctgc cgtggttcgc cggccgtcat ccacagccga
     3061 atacacgacc cagatcagca gcagcccgtc cggggcggcg gtggtgttcg acaacaatcc
     3121 ttccatccat tgccggaccc cgtgcgccat tcctttactg gcggggcggc acacactttc
     3181 cgcggaactg gaggggtatc gccaggcatt gcggattttt gaggtccccg ccgaacgcga
     3241 ggttttcgtt ccgctggagc ggcgcaccgg acaactcagc attcgttcga atcctcccgg
     3301 tgcctccatc tccatcaatg gtgagccgcg cccggagaaa acacctgtca cgctcagttt
     3361 gccggccggg aagtatcgcg tgggcgtggg gggtgccgga catccgcagg aagtgacgga
     3421 cgtggagatc aaggatggcg atctgaagaa tctcgtcatt gactggagcg gcaaagggta
     3481 acccagccgc cgggctttcg cgcgcgcggc acgctgatac ataatgcgat cagcgaaacg
     3541 aggtgcatat gcggatggcg tctctgggtt tggctgtcgc gtccgccacg ctggcggctg
     3601 cggctacgcc gatgaaaatt cgggtggagc cggtgacgga taccgttcgc gctggggaat
     3661 ccccgtctct tgtcgttcaa ctgatggatg cggacaatcg gccagccccg gcgccgaagg
     3721 acctccccgt gaagatctcc gcgcggctcc cgtcgaatgc agcggaggag gtcgccggga
     3781 ttatcatccg ggcgggccag tcttcggcaa ccattaagct tccgtctccg cgggcaacag
     3841 gacccgttta ttactgggcc aggcagcagg agttgatcac cggcggaacg ttcgtgatgg
     3901 tagagagcgg ccagccaccg gctgcgggtg atgtacccgc cagcccttac gttgtgacgc
     3961 tgcgatatag cccgcaacgt gagttcctcg ccgacggcaa agatcctgtg acggtgcatg
     4021 cttttgtctt ctcgcgcaat gacacaccat tacctgcgtt ccgcatcacc ctgttcgaca
     4081 gcagcggcac catcaatccg gtcccgctcg tgataccgcc gggacagggc agcggcacgg
     4141 caacgctcac atcgaaatca ccaggggaag tgaaggtgga atacgtcagt tccgtgccgc
     4201 gtgcgaatgt tgatggcgac cccgtgctga agatcccgtt cggtccgccc attaccgctc
     4261 tacaactgag cgtgaacccg cgagagattt cgcttgtcga ccgcgcggac ctggtggtac
     4321 gcctggtgga cgaacacggg acgccgctcg ccactacacg gccgagaacg atttcgcttg
     4381 ctctggaccg cggccgtggt gcgatcgaga aatccgagct tacgattcca cccggctccg
     4441 cggaggtgag aaccggcttt tcgcccttgt ggtgggggac aatgggcgtt gccgtgtcca
     4501 gtccgaatct ctttaccgtg accgcgccga tggaggtcaa gctgccgttt gctcttctcg
     4561 gcatctccct gctgggcggg ttggcaggcg cctacgtctt cctcttgcga aatcgccgta
     4621 cgaagccgtg gagggtattt gtgggagcgc taaccggctt cgttttttac tgggcctgcc
     4681 tctttctttc gctggtgggg ctgccgggaa cgattgtgct gaatccgctg agcgcatttg
     4741 tgctctcggt attgggtggc tggatgggcc cggaaatttt cgagagattg ttagccagat
     4801 tcggttttgc gaaggaaaag gccaaggctc gcggagcggc atagctccac gcgcgatgaa
     4861 ggcaggtacg atgaaagaag ttgcgctggg ggaaaagcgg tttcgtgatt cgaacaattc
     4921 tatacgtatg tgttttgctt tgcctgtgcg aaaccggcgt gctgcgttcg gccgaagacc
     4981 ccccggcaca gccgattcct tttagccata agcagcacgc ggcggctggg gccgcctgcg
     5041 tggattgcca cacgacggcg aagaaagccg cacgcgccgg gatgccggat accgccaggt
     5101 gtctgctctg ccaccgcagt gtgaagcagg acagccccgc cattgtgaaa ctccgtaaaa
     5161 tgcacgaaga caaggaaccg atcgaatgga tccggctgta ccaggttccc gatttcgtat
     5221 ttttcagtca cgagaaacat ctcgcggcgg gcgcggcctg ttccgaatgc cacggtcccg
     5281 tggaacagag agaagtattg acgaaggaag tgcccaccac aatggccgcc tgtatgaatt
     5341 gccacaaa
//
LOCUS       DASTMK010000403         5978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_244145, whole genome shotgun sequence.
ACCESSION   DASTMK010000403 DASTMK010000000
VERSION     DASTMK010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244145"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1525
                     /locus_tag="VFQ79_17325"
     CDS             113..1525
                     /locus_tag="VFQ79_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0141488.1"
                     /translation="MATGESKAPNELVELLSQKVIGQPNALKFIVPYIEMYQAGLAPE
                     GRPVGVFLLLGPTGTGKTRTVEVLAEVLHGSQSNYLRIDCGEFQIEHEVAKLVGAPPG
                     YLGHRETQPMLSQQKLASVTSERSDLALVLFDEIEKAAPSLTRLLLGILDKATLRLGD
                     NTAVNFEKTLIFLTSNLGAREMMKELNPSFGFQPGTPRDKEELGSKLESIALAAVRKK
                     FSPEFVNRIDAVVTYRPLDAESLATILDQQIGELQQHVNTRLGARCFRIEVTPESRKF
                     LLEKGTSQEYGARELKRTIHRFLTQPLATLVASGQIEPGAHVAVEVDSSGDRLQMRTV
                     AVDTPAQPAPANPTVLIVDDNRDLLRFLEHLMVGAHWRILTAETGQQALAAVASQTPD
                     AALLDYMLPDLNGLELALQIQKKLPRLQIILMTGANLAAEEELVCRQNGFPLLQKPFL
                     AQDIMSLIRNRLSGAAACPA"
     gene            complement(1497..2684)
                     /locus_tag="VFQ79_17330"
     CDS             complement(1497..2684)
                     /locus_tag="VFQ79_17330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0141489.1"
                     /translation="MLIVAAILAIFVYGMIAAMLGTILPYLSTAFNLTPQENSYIALA
                     QGLGLTLASISVGPLIDNKGKKTGILLGLSMIAIALFGLPNSTGYIMIMALILLLGLG
                     GGILVTGANALVSDISDKRRASVLNFLNLFFGLGGFATPFIAGNFFEGNAIQLCYFIA
                     ILVVVTLTVHALTKMPPPSGERGFKFAEVGDVLGRPVLYLLAGFLFLYVACETGVWNW
                     LPRHLMAQGIPESRSMNILSLGFALGLLIGRMVVAPILIKVAPATVTLYSAVLMMITT
                     FAMLQTNDPGVAWIAVFCAGVAMAPVFPTTLAMVGNAFPKMTATAMGVVITCGWIGLV
                     VSSPIIGAIAGVDPTRLKTALLLLPIFSVAMIVVNLALRPMLKSPVPAVETPATPGRP
                     PRR"
     gene            complement(2726..3502)
                     /locus_tag="VFQ79_17335"
     CDS             complement(2726..3502)
                     /locus_tag="VFQ79_17335"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0141490.1"
                     /translation="MSPRNLLLEFDRDVAVLTLNRPEKRNALSLDLMLEFMACLDEAA
                     RHAGVRAVIVRAAGKVFCAGHDLSEMVGRTVVEYRRLFDVCTELMETIQSIPQVVIAE
                     VQGLATAAGCQLVASCDLAIASADAAFATPGVRIGLFCTTPMVALTRSIGRKRAMEML
                     VTGRTVDAFTAMDWGLVNRVVQPAELGVETRKLAAQVAQSSDFVTGLGKQAYYAQIDL
                     DQPKAYAYAKEIMTMNSLAADAQEGISAFLEKRPPCWTGK"
     gene            3564..4280
                     /gene="rlmB"
                     /locus_tag="VFQ79_17340"
     CDS             3564..4280
                     /gene="rlmB"
                     /locus_tag="VFQ79_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU0141491.1"
                     /translation="MAVLSGIHPIREALQAGRLFDRVLIAKGAGGARLQELIELCRLA
                     SVPVRFEPRSALDRIAGSSAHQGVVALGAAKGYAALEDVLPSGGLLVVLDGVEDPHNL
                     GAIIRTAHAAGASAVCVPERRAAGLTDVVAKAAAGALEHLPVVRLGNINQSLELMKKR
                     GFWIYGIDERGPEMYDQVDYASPAALVFGAEGHGLHQQVRNHCDVLVRIPMAGRIASL
                     NVSVAAGVVLFEWKRRSKPA"
     gene            4298..5965
                     /gene="ggt"
                     /locus_tag="VFQ79_17345"
     CDS             4298..5965
                     /gene="ggt"
                     /locus_tag="VFQ79_17345"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0141492.1"
                     /translation="MRWVLLLLVFLQFSGSPATCAQPVRARHAMVVTREPHATRAGLD
                     VLRSGGNAVDAAVAVGFALAVTHPAAGNLGGGGFLLARFADGRTTFIDFRERAPGAAS
                     RNMYLDESGALTRDSLTGYRASGVPGTVAGLEYAHRKFGAKPWSELLRPSLQLASDGF
                     PVSYGLAQSLQATKVLSRFPESNRIFLRDGRYFEPGEILKQPELARTLERIAREGSKD
                     FYRGETAQLFAKAMKANGGTITLEDLRNYRPVERKPLTGAYRGYQIVTAPPPSSGGVG
                     ILQMMGVLENSGYAKSGPGSAAAIHFIAEAMRRYYADRSKYLADPDFFEVPVSKLLNR
                     RYIETLRASIDPERARPSTEVHPGDLKAYESAETTHYSIVDAEGNAVAVTYTLNGSYG
                     SGVTLPGLGFLLNNEMDDFSAKPGEPNMFGLIQGEANAIQPNKRPLSSMTPTILLRDG
                     SLYMVAGSPGGSRIITTVLEVILNVVDFGMNIQDAVNWPRFHHQWQPDKLFVEWRHSP
                     DTKALLQSRGHQVEDIRSMGEVSAILVDRGWLEGAADPRSEGNAEGY"
BASE COUNT         1267 a         1741 c         1848 g         1122 t
ORIGIN      
        1 aaacaatcgt gtgcggtctg tcatggccgg cgatttacct gccttggctg ccattgatta
       61 ttttcgagac cagcgggccg acagatataa tgcagagtaa agggggctga aattggcaac
      121 cggcgaatcc aaggcaccga atgaattagt ggagctttta tcccaaaaag tgattgggca
      181 gccgaatgct ctgaagttta tcgttccata tatcgagatg taccaggccg gactggcgcc
      241 ggaaggccgt ccggtagggg tgtttctgct gctgggaccg accggcaccg gaaagacacg
      301 caccgtcgaa gtgctggcgg aagtgctgca tggaagccaa agcaattatt tgcgcatcga
      361 ctgcggcgag ttccagattg agcacgaggt ggcgaaactg gtgggagcgc ctccggggta
      421 cctgggacac cgggagaccc agccgatgtt gtcgcaacag aagctggcgt cggtgaccag
      481 cgagagatcc gatttggcgc tggtgctgtt tgacgaaatt gagaaggcgg caccttcttt
      541 gacacggctg ctactgggaa ttctggataa agctacgctg cgcctgggcg ataataccgc
      601 cgtgaatttc gagaaaacgc tgatctttct gaccagcaat ctgggtgcgc gcgaaatgat
      661 gaaggagttg aacccgagtt tcggttttca acccggcacg ccgcgcgaca aggaagaatt
      721 gggcagcaaa ctggagagca tcgcgctggc ggccgtgcgg aaaaagttct ccccggagtt
      781 tgtcaaccgg atcgatgcgg tggtgacgta ccggccgctc gatgcggagt cgctggcgac
      841 gattctcgac cagcagatcg gcgaattgca gcagcacgtc aacacgcgtc tgggggcgcg
      901 ctgcttccgc atcgaggtta ctcccgaaag ccgcaagttt cttctcgaaa agggcaccag
      961 ccaggaatat ggagcgcggg agttgaaacg aaccatccac cgctttctga cgcaacccct
     1021 ggctacgctg gtggcctccg gacagatcga acccggcgca catgtcgccg tggaagttga
     1081 ttcttccggc gaccggctgc aaatgcgaac ggtggccgtg gacacaccgg cgcagcctgc
     1141 accggcgaat ccgaccgtgc tcattgttga cgacaaccgc gacctgcttc gctttctgga
     1201 gcacctgatg gtaggagcgc actggcgaat tctgacggcg gagaccgggc agcaggcgct
     1261 ggcggcggta gcgtcgcaaa caccggacgc cgcgctgctc gactacatgc taccggacct
     1321 gaacgggctc gaactcgcgc tacagataca gaagaaactt ccgcggcttc aaatcattct
     1381 gatgacaggc gcgaatctgg cggccgaaga agagttggtt tgcagacaga acggcttccc
     1441 gctgctgcag aagccttttc tggcgcagga cattatgagt ctgatccgca accggcttag
     1501 cggcgcggcg gcctgcccgg cgtagccggc gtctcgacgg cgggtaccgg actcttcagc
     1561 atcggtcgaa gggcgaggtt cacgacgatc atcgccaccg aaaaaatcgg cagcaacaac
     1621 aaagctgttt ttagccgggt ggggtcaacg ccggcgatcg ctccgataat cggagaactc
     1681 acaaccagtc cgatccagcc gcaagtgatc acaaccccca tcgccgttgc ggtcatcttc
     1741 gggaatgcgt ttcctaccat cgccagcgtc gttgggaaga ccggggccat agccacaccg
     1801 gcgcagaaca ccgcaatcca ggcgactccc ggatcgttgg tttgaagcat cgcgaaggtg
     1861 gtgatcatca tcaggaccgc ggagtagagc gttaccgtgg cgggcgcgac tttgatcaga
     1921 atcggcgcaa ccaccatacg gccgatgagc agcccgaggg cgaagcctag agacaggata
     1981 ttcatggaac gggattcggg aatcccttgc gccatcaagt ggcgtggcag ccagttccag
     2041 acacccgttt cgcacgccac atacagaaac agaaatcccg ccagaaggta caacactggc
     2101 ctgccgagaa catcgcccac ttctgcgaac ttgaatccgc gttcaccgct gggcggcggc
     2161 attttagtca gggcatgaac ggtgagggtg accacaacca ggatcgcgat gaaatagcag
     2221 agctggatgg cattgccctc gaagaaattg ccggcgataa acggtgtagc gaatcctccc
     2281 agcccgaaga agaggttgag gaagttcaga accgatgcgc gccgcttatc gctgatgtcg
     2341 ctgaccaggg cattagcgcc ggtaacaaga atcccgccac ccaagccaag cagcagaatc
     2401 agggccatga tcatgatgta gcccgtggag ttcggcagcc cgaacaacgc aatggcaatc
     2461 atagaaagcc cgagcaggat tcctgttttc ttccccttgt tatcgatcaa cggacccact
     2521 gatatcgagg ccagagtcag gcctagcccc tgggccagcg cgatgtagct gttttcctgc
     2581 ggtgtcagat tgaacgcggt cgataagtac gggaggatcg tgccaagcat ggcggcgatc
     2641 attccgtaaa cgaagatggc gagaatagcg gcgacgatga gcatatgtcc tcggactcac
     2701 gatcttatca gaaactattc gagaatcatt tgcccgtcca gcaaggaggg cgcttttcaa
     2761 gaaaggcgga aatgccctcc tgcgcgtccg ccgccaacga attcatggtc atgatttctt
     2821 tggcgtacgc gtatgctttg ggctggtcca gatcgatctg cgcgtaatac gcctgcttgc
     2881 ccagccctgt cacgaaatcg ctggactgcg ccacctgggc ggccagcttc cgcgtttcca
     2941 cgccgagctc ggctggctgg acaacccggt ttaccaggcc ccaatccatc gcggtaaagg
     3001 cgtcgaccgt ccggccggtc accagcattt ccatggcccg cttacggccg atggagcgcg
     3061 tcaacgccac catcggggta gtgcaaaaca ggccgatacg tacgcctgga gtggcgaacg
     3121 cggcatcggc gctggcaatg gcgagatcac agcttgccac caactggcat cccgcggccg
     3181 tggcaagccc ctgcacttca gcgatcacca cctgcgggat agactggatt gtttccatca
     3241 gctccgtaca gacatcgaaa agccgccggt actccaccac cgtacgcccc accatttcgg
     3301 acaggtcgtg cccggcgcag aagacctttc cggccgctcg aacgatcacc gcgcgcacgc
     3361 cggcgtgccg ggcagcctca tccaggcaag ccatgaactc cagcatgaga tcgagcgaca
     3421 gtgcgttgcg cttttcagga cggttcaatg tcaggaccgc aacgtcgcgg tcgaattcga
     3481 gcagaaggtt tctcggtgac atagctaccc cgtgaccagt gtagacaaga catcccgcgt
     3541 tccgcgaacg ctaccatcgg attgtggcgg tactaagcgg gattcatcct atccgggaag
     3601 cgttgcaggc cggtcggctg tttgaccggg tgttgattgc gaaaggtgcg gggggcgcaa
     3661 gacttcagga attgatcgag ctttgccggt tggcgtcggt gcccgtacgc ttcgagcccc
     3721 gaagcgcact cgaccggatt gcgggcagca gcgcgcatca gggcgtggtc gcattgggag
     3781 ccgcgaaagg gtacgcggcc ctggaggatg ttctgccttc agggggactg cttgtggtcc
     3841 tcgatggagt cgaagatccg cataacctcg gcgcgattat ccgtaccgcg catgccgccg
     3901 gtgcgagcgc ggtctgtgtc ccggagcgtc gcgccgcggg ccttaccgac gtggtagcga
     3961 aagcggccgc gggtgcgctg gagcacctgc ccgtcgtgcg cctgggaaac atcaaccagt
     4021 cgctcgagct gatgaagaag cgcggtttct ggatctacgg tatcgacgag cggggcccgg
     4081 aaatgtacga ccaggtggac tacgcctcac ccgcggctct tgtattcgga gccgagggcc
     4141 acggcctgca ccagcaggtg cgcaatcact gcgacgtgct ggtgcgaatt ccgatggcgg
     4201 gccgtatcgc gtcgctgaat gtgtcggtgg cggcgggagt cgtgctgttc gaatggaaac
     4261 gccgctcaaa gcccgcatag taaaatcgag tgtcaatatg cgatgggtgc tgttactcct
     4321 ggtgttcctg caattttcgg ggagccccgc cacctgcgcg cagcccgtgc gcgcgcgcca
     4381 cgcgatggtt gtcacgcgcg aaccccatgc aacgcgcgcg ggcctggatg tgctgcgttc
     4441 cgggggaaac gccgtggacg cggccgtggc ggtgggtttc gcactggctg tgacgcatcc
     4501 ggccgccggc aatctggggg gtggagggtt cctgctggca cgatttgccg atggcagaac
     4561 cacgtttatc gatttccgcg aacgtgcgcc cggcgccgcc tccagaaaca tgtatctgga
     4621 cgagagcggg gcactgacgc gcgacagcct caccggttat cgcgcctcgg gcgttcccgg
     4681 gacggttgcg gggctcgagt atgcccaccg gaagtttggc gccaagccat ggtccgaact
     4741 gctgcggccc tcgctccaat tggcttccga tggttttccc gtctcctacg ggctggcgca
     4801 gtctctgcaa gcaacgaagg tattgagccg ctttcccgaa tcgaaccgca tttttctgcg
     4861 cgacggcagg tatttcgaac cgggcgaaat attgaagcag cctgaactcg cccgcacgct
     4921 ggaacggatc gcgcgggaag gctccaagga tttttatcgc ggcgaaaccg cgcaattatt
     4981 tgcgaaagcg atgaaggcca acggcggcac catcacgctc gaagacctgc gaaactatag
     5041 gcccgtagaa cggaaaccgc tcacgggcgc gtaccgtggt taccagatcg tcaccgcacc
     5101 tccaccgagt tccggcggcg tcgggattct gcaaatgatg ggtgtgcttg aaaacagcgg
     5161 atacgcgaaa tcgggccccg gttccgccgc ggctatccac ttcatagcgg aagcaatgcg
     5221 gcggtattac gcggaccgaa gcaagtacct ggccgatccc gactttttcg aagtaccggt
     5281 ctcgaagctt ttgaaccggc gctacatcga aaccctgcgc gcttccatcg acccggagcg
     5341 cgccaggccc agcaccgaag tccatccggg agatttgaaa gcctacgaga gcgccgagac
     5401 cacacattat tcgattgtgg atgccgaagg aaacgccgtc gccgtaacct acacactcaa
     5461 cggtagctat ggttccggtg tgacgctgcc cgggctcggc tttctgctga acaatgagat
     5521 ggacgatttc agcgccaagc cgggcgagcc gaatatgttc ggcctcatcc agggagaggc
     5581 gaacgccatt cagccgaaca agcgtccctt gtcgtccatg acgccgacca tcctgctgcg
     5641 cgacggtagc ctatacatgg ttgccggctc accgggcggt tcaaggatca tcacgaccgt
     5701 gctggaagtc attttgaacg tcgttgattt cggtatgaac atccaggacg ccgtgaattg
     5761 gccgcgcttc catcaccagt ggcaaccgga caagctgttt gtggagtggc gccactcccc
     5821 ggatacgaag gcattgttgc agtcgcgtgg gcatcaggtg gaggatatcc gttcaatggg
     5881 tgaggtgtcc gccatattag tggaccgcgg ctggctcgaa ggcgcggccg atccccggtc
     5941 cgaagggaac gccgaaggct attagatcaa atgaagca
//
LOCUS       DASTMK010000404        21883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_436484, whole genome shotgun sequence.
ACCESSION   DASTMK010000404 DASTMK010000000
VERSION     DASTMK010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21883
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436484"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VFQ79_17350"
     CDS             complement(<1..968)
                     /locus_tag="VFQ79_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU0141493.1"
                     /translation="MPTPAAAEPVRLIFDTDMGNDVDDAMALSMIHALQNRGECELLA
                     VTVTKDNPYAAPFIDLMNTFYGRGGIPIGVVQKGVTPEDGKYNRAVVTAAASGGGPRY
                     PHDLKSGKDAPDAVAVLRKTLAAQPDNSVVIAMVGFSTNMARLLASGPDAMSPLAGTE
                     LVRRKVRLLSAMAGGYTEELAKKRYKEFNIVRDIPSAQKVFAEWPTPIVASGWEIGYA
                     IHHPAVSMREDYGYVDHHPLVEAYQYYRGLTNDQPTYDLTSVLYAVRPNRDYFALSGP
                     GRIVVEPDGFTRFESASGGQHRYMRVGNDQVVRVREAQALLCSEPPR"
     gene            complement(1001..2395)
                     /locus_tag="VFQ79_17355"
     CDS             complement(1001..2395)
                     /locus_tag="VFQ79_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU0141494.1"
                     /translation="MSFDMPPEEFRKTAHEVVDWIADYLEGIRDYRVMPIMEPGSLVR
                     SLPKSGPEQGEPAGRILEDFRNLVIPAVNHWNHPRFFGYFSVSASHPGILGEMLMSAL
                     NVNGMLWKSCPAATELEQVTLGWLRQWLGLPEEFFGIIYDTASISTMHAIAAAREMAD
                     PEARERGGSQNLVLYCSEQAHSSVEKGAIAIGIGQKNVRKIAADDRFRMRTDLLKAAI
                     EEDVRASKKPFCVVATVGTTSTTSVDPVSEIVPLAEEHGMWLHIDGAYGGTAALVPEH
                     RHILDGAARAHSLVMNPHKWLFTPQDASVFYTRKPDILRRAFSLVAEYLRTAEDERVV
                     NYMDYGVQLGRRFRSLKLWFVLRNFGREGIIEILRNHIAWAKELACAIDADPRFEVAA
                     PVPFSLVCFRFKGSDEQNRLLLDRINADGQAFLSHTVLNGRFVLRLAIGDIKTTREDV
                     METWKCISKLAETL"
     gene            complement(2392..4074)
                     /locus_tag="VFQ79_17360"
     CDS             complement(2392..4074)
                     /locus_tag="VFQ79_17360"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0141495.1"
                     /translation="MSERSVKLFLFLFCALALRAQVYPAGPQVLTFFSGIDDSDQPYA
                     LYLPKNFDAAKKYPLVVSLHGAWSNHRLNLRRVFGKGNRAGETDPEATRYFPALRDVD
                     FLVASPYSRGTMGYQGIPEQDVYDVLADVKRRFPIDEDRIYLTGLSMGGGGTFWLALT
                     RPDIWAAIAPVCAAVPEGSLDLAPNLLNIPVHLFHGDADPAVPVEQSRKWNHELLRIG
                     VNVQYTEYPGVRHNSWDLAYKDGAIFDWFSQFRRNRFPDEVRFATRNYKYNSAYWVQL
                     DGLTPGDLAKISARFQNRNELVVETSGVKGFTLTPAGHSSFAVGRAVSVTIDGAVLKL
                     KGTEKLSFRKAEKGWQPGRYTPPPGEKRPGSEGPIGEAVAARHVYVYGTADSPSPEEL
                     NRRRRQAEEAAEWSTPRLKLLVNFRTVADKDVGESDLKGSNLVLFGTRETNSLIARLA
                     GDAPMALNAGAADFGLVFVMPLGEHYALVNSGLPWWTGADRAQRAGLRFMPPPYRLLL
                     SFGDYILFKGSLDNVIAEGRFTSEWTVPPPEAEKMKATAAVTLRTAHREPAK"
     gene            complement(4130..5020)
                     /locus_tag="VFQ79_17365"
     CDS             complement(4130..5020)
                     /locus_tag="VFQ79_17365"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0141496.1"
                     /translation="MASFQGIYAAPVTPRRASGHEIDLAAMLEFVDYLGESGVDGIAL
                     LSPASEFVHFNPEERVRVTALATKRSRLPVLANCSHSTLDGAVFIAQEISGAGVAGLL
                     LMPPYFFRYEQPEIERFYLEFMDLCGAPPPVYLSNVPSFTSEIAPPTAARLLATGRFA
                     GIHDASAHWGAFDSNAGFPCIAGCDAAYVGARSHGAAGAISEIACVVPELMVALDRAI
                     AAGAPDAVARLEARLNELLGWIDSFPSPVGIREAAVARGLKAGPPAVPLGPENVQRAG
                     EFRAWFQAWLPAVQKECKDV"
     gene            5121..6311
                     /locus_tag="VFQ79_17370"
     CDS             5121..6311
                     /locus_tag="VFQ79_17370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141497.1"
                     /translation="MDVSRRSFVAGCAGVASAGAQTSSPRPVRIGVVGGGFGSSFQWH
                     LQPDCKVEAVCDIRPDRLQRLSEVYRCGAAFTNFREMLAKAKLDAVGVFTPAPLHAWM
                     AMESMKAGKHVISAVPAGITVEELERILETVRSTGMRYMMAETSYYRPEIMTCMEMQR
                     KGEFGTIFYSEAEYHHEGLIPLMFDKIGNPTWRYGFPPMLYPTHCTGMVVPVTGERLT
                     SVQAVGWGDGHEVLQTNMYTNPFWTTTGFFRTSKGHSSRISVFWHVAAGGTERAQFYG
                     DRLSYIMQRPEGSPNTLIKISKDGKTVIDANGYPEGDVKIEAFKQPDHLEKLPPSMRV
                     KTGHGNSHTFITQEFIRAIVEDRHPAVNIWEAIAYTLPGIVAHQSALRGGEPMKIRDY
                     GTPA"
     gene            complement(6368..7603)
                     /locus_tag="VFQ79_17375"
     CDS             complement(6368..7603)
                     /locus_tag="VFQ79_17375"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141498.1"
                     /translation="MVPLQVALIGGGMIAHDQILPSLYHLQRQGVIGEISVCATEARF
                     LKSLVDSENISKAFPGQSFRPFPDLSDTGVHLDLYKQVIAGLPPRNIVAVAVPDQLHH
                     AVIMEALRHDQHVLTVKPLVLTAAEATEIEQEASRRGLLVGIEYHKRFDDRSLMARRR
                     YRDGWFGEFRLGTACLLEKWYYRHSNFQNWCTVENSDAFTYIGCHYVDLVHFITGLLP
                     VSVSVYGIKDRYPNGNEGFLWTDARVLWNNGASLNVQNALGFPDDAPGTNTQGMVLYC
                     SGREKGGLLGHSDQYRGLEYSYVRPPDGPGATVYAEPSPDYFQYVDLGGGGLVPVGYG
                     YRSVDFIIRAAVRLESQAGTLEERQQMLRQFDEEGVMATPANSRYNELVIEAGRLSIL
                     NGGREVRIQYEGKPAVALA"
     gene            complement(7603..8991)
                     /locus_tag="VFQ79_17380"
     CDS             complement(7603..8991)
                     /locus_tag="VFQ79_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0141499.1"
                     /translation="MAREYELTPRRVPRIETALRRIVTELPVPASVPVLEKLHQYEPV
                     AMRGQPPIVWDHAEGFQVFDAHGNCWIDWSSGVLITNAGHGRREIIEAVSGQAASKLL
                     TNYCFPSEIRARLVEKIASLLPDPLKKVFLLTTGSEAVECAIKLCRTNGVKVGGRSKN
                     VIVSYDKAFHGRTLGSQQAGGIPALKDWIVNLDPGFVQIPFPDGFRTENTSFEFFEEC
                     LSKAGIKPADVAGVVLETYQGGSASFAPPEYMKRLRKWCDANGALLVCDEVQAGFGRT
                     GTMWGFEHYGIVPDLTTFGKGISSSLPISAVAGRPDIMDLHPPGSMTSTHTGNPVCCA
                     AALASIEIVERENLVENARKQGTLLLSLLNDLKAKHPEIGSVDGKGLVVGLSCVKPGT
                     KEPDGDLAWDIVERSIAKGVLMFSPVGFGGGTVKICPPLVITEEAIRESAAAFSEAFS
                     EALAARKQEAAV"
     gene            9372..11537
                     /gene="glgX"
                     /locus_tag="VFQ79_17385"
     CDS             9372..11537
                     /gene="glgX"
                     /locus_tag="VFQ79_17385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEU0141500.1"
                     /translation="MSDSDKNERPPRTVLPGRPYPLGATWDGAGTNFALYSHSGTAVD
                     LCLFDDRGQEEQIPLREQTNFVWHCYLPAAPPGQLYGYRVHGPYEPKRGLRFNPAKLL
                     LDPYATAVSGEVNWEAPLFGYQLGHPDADLSCDSGDSAWGMPKGAVVSPFFDWENDRP
                     LRTPLNDSVIYEVHVKGFTIQHPDIAQELRGTYAGLGSPPAVQYLKKLGITAVELMPI
                     HDMLDDKHLVDQGLKNYWGYNTTNFFSPAARYASAGDKGGQVAEFKSMVKALHREGIE
                     VILDVVYNHTSEGNHLGPTLSFRGIDNLTYYRTNDDARYYMDYTGTGNSLNVRHPQVL
                     KLIMDSLRYWVEEMHVDGFRFDLAATLARELHDVDRLSAFFDIIHQDPVLSQVKLIAE
                     PWDVGEGGYQVGNFPILWAEWNGKYRDVVRRYWKSEDGTLAELGYRLTGSSDLYQRDG
                     RRPSASINFVTAHDGFTLNDLVSYNEKHNEANGEENRDGSNDNHSWNMGVEGPTSDPE
                     IIELRERQKRNLLATLMLSQGVPMICGGDEISRTQRGNNNAYCQDNEISWLDWTPEAG
                     KRALFEFTRELIRLRLEHPNLHRRKFFQDREIRGTIEKDVSWFRPDGEEMTDEEWSSG
                     WVRCIGVRLNGETLNDSDSKGAPLHDDTFMLLLNPHHEPIDFYLPEPDKQTHWEMLVN
                     TIQPGFYQNQCIKSGEPVNLARQTLVVLQQVAPHVPTVP"
     gene            11607..13895
                     /locus_tag="VFQ79_17390"
     CDS             11607..13895
                     /locus_tag="VFQ79_17390"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HEU0141501.1"
                     /translation="MLTVALCQSPDREALPQEWGYRPADGATVPLNPPALSWVPEKGA
                     AGYTVQWANNPEFADPATVPNLRWTVYTHHQPLKPGTWWWRYRIASEEERQSSWSRAR
                     QFIIPAEAAVFPKPTMEDLRRRIPTGHPRLFVRAEDLPKWKQWAAGGGKEPYGRLLAR
                     ADALLRAEPTPEPTVRANPYDAATRQYWWSNRVETIEALQEAEILAFAWLLTGNSKYG
                     EQAKRFTLKLAEWDPDGPTRFSLNCEAAKPLVHRLARAYDWAWPLFTPEERSKIRAVL
                     LRRARDAWNSGEVKQGGGHLAEPYSSHGNRTWHKLAENAIATLGETPESEEFLEYAMA
                     KFFGAYPVWSDDDGGWHEGLNYLGGYMSKTRWWIDIARTAMNIDAFQKPFFTRIGDYA
                     MYSAPPGTPNLGFGDLAIGPVSRGWSFLHYYVRNTGNAHWAWWLQQWDVGETNEEPVL
                     DFLWSAAPRPNPQAPSGVPPSKLFEGTGVAILNSNLLDGRENVQLRFKSSPMGRWSHG
                     LDPQNSFTLNAYGSALLVNNVYRDLYGSPFHSKWVWSTRAQNAVLINGEGQRPRSPAH
                     DGRIVKAEFQPGLDYVSGEAAPAYAQNLKRARRHIVFVKPDVAFIADEIQAAKPGTYQ
                     WMLHAPQAFQVDESQQRLVLDRGAAGVIVEYAAPQPLKLRQWTGYDPEPDAEYLASVK
                     RPPIPPQWHVEASADKPTDDVFTVTVLRVFRQGQTPGSQMRVSRDGGTLRIAAGDVEA
                     AFEQRRIVARKGDRRWTIALPE"
     gene            complement(13902..14246)
                     /locus_tag="VFQ79_17395"
     CDS             complement(13902..14246)
                     /locus_tag="VFQ79_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141502.1"
                     /translation="MKRTFIVCIMLLVGLLPFRPAASQGQQQSKVKPAKIESLQNIGV
                     SRSESGQPAFLPREWGRLVSVQQIEGDRYVLFLQGDDGAIYLVRLLQQGQYLYLDTYD
                     QGGVALVIRREP"
     gene            14331..15173
                     /locus_tag="VFQ79_17400"
     CDS             14331..15173
                     /locus_tag="VFQ79_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0141503.1"
                     /translation="MTSRRAFLSVAGSALPLAAKQKFSGPLGLEIYSLRRQAQKDLPG
                     TLAMIREMGFKELEVGGYYGRTVSEFKRMLAANGLAATSMMAGWDQLSNSTGKVAEEA
                     RTLGSGYVVCGSIPHKKPFSADDAHRAADHFNKWGEALKSAGLQFCYHAHGFEFGASP
                     DGTLFDTMAKRMDPKVANFQMDVFWIAFGKEDPAKLLERYAGRFPLMHLKDIRKGEAK
                     TGNPGDVVEEASVPLGTGEIDWPSVLSAARKTGVRRYYIEEEHPDAVKQIPKTLRYLE
                     NIRF"
     gene            complement(15281..17257)
                     /locus_tag="VFQ79_17405"
     CDS             complement(15281..17257)
                     /locus_tag="VFQ79_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0141504.1"
                     /translation="MRRRDLLSGLGLGGYSLVVLADQIPQGPAPAVSERLRREFDRIA
                     PPDPQRRKAEAEPNMAVVDLETDVFIAGGGLAGVCAAVAAVRNGAKVVLVQDRSRLGG
                     NSSSEVKMHVVGANSHKGRPGWREGGLIEEFRLDDAANNPQRCWELWDLLLYDKVVSE
                     PNITLLLETILYSATMQGSRIEQVMARCDKSEHLYRIKAGLYVDATGDSRLGLEAGAE
                     YRTGRETRSEFNESLAPETADQETLGSSILFTSRLHRRPMPYTPPKWARKVTKEHLRF
                     RKVSPENWEYGYWWIEWGGDKDTIRDNERIRFELLSIVTGVWDYIKNSGDYPDSAHWA
                     LEWVGMMPGKRGSRRLVGDVILTQGDLMRGTWPDAVAMGGWPMDDHPPGGFDRPDLPP
                     NTVIHTDEVFDIPLRALYSRNITNLMMAGRNISASHAAFTSTRVMATCASIGQAVGTA
                     AALCRERGVTPRQLARDRQLVSGLQQLLLRQDQSIRTVSNRDTRDLARTAAIKASGEV
                     GEAKAALVTDGIDRDIPANKRTGKPGEVHHWAANVGSREAWIELSWRQAQRLREVVLK
                     FDTGFQRELTLSASDSISRAIVRAPQPETVRDYTVTVTGADGRQHTVATVTGNHQRLN
                     RVTFEPVDALSVRITATKTNGAEEVRIFEVRCYA"
     gene            complement(17264..18424)
                     /locus_tag="VFQ79_17410"
     CDS             complement(17264..18424)
                     /locus_tag="VFQ79_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU0141505.1"
                     /translation="MFSVIPSVVMGAVPAAVRIDDDIPGGNIIVDAVDGDTVSVRQDL
                     RDTEGDWFYWAFRVRGAAGRKMTFRFPGRAPLSALGPAVSLDRGHTWQWLGADSIHQA
                     SFTYTFPARAEEVRFSVNIPYFEADLVRFLDRHRGSPHLKAGVLAKSRKGRDIEMLRV
                     GNFENPKMRAILTARHHACESLASYSLEGILAGIVEDTAGTWLRENVEFLVVPFMDKD
                     GVEDGDQGKNRRPHDHNRDYDGESIHPSVKALRELTPKWSDGRLRFAMDLHCPYISGK
                     GNEEIYFVGSRHPEIWREAVRFSKILESVQSGPLVYRAENNLPYGEGWNTDANTKGLK
                     SFGMWAAEQPGIRFASTLEIPYATAGGTAVTAASARALGIDLAKAIGRYLEG"
     gene            18503..18865
                     /locus_tag="VFQ79_17415"
     CDS             18503..18865
                     /locus_tag="VFQ79_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEU0141506.1"
                     /translation="MVVSEALMHELSIATSIVELAEEEAERRGVRVVAVHLTLGQLSG
                     VVTEALAGSYDIATAGTPLEGSRLVITEAPVVVFCPACRERRTLPSVQLFQCPQCGTP
                     TADVIEGRELQVTALEVE"
     gene            18865..19533
                     /gene="hypB"
                     /locus_tag="VFQ79_17420"
     CDS             18865..19533
                     /gene="hypB"
                     /locus_tag="VFQ79_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEU0141507.1"
                     /translation="MKPEPRLVEVRRNVLKHNDLVARELRARFQREGISVVSLVSSPG
                     SGKTRFLESTLSRMNGRHRVAALVGDLATENDAVRLARSGAPVKQIVTGTVCHLDAGM
                     IRNSLEEWHLADVNYLFIENVGNLVCPASYDLGEDVRAVLMSVTEGEDKPLKYPTIFN
                     GADVAVITKMDLCEAAEFDLEAARANIQAVRPGLRVLCVSAKTGEGMEEWMEYIESLT
                     PAQK"
     gene            complement(19588..20616)
                     /locus_tag="VFQ79_17425"
     CDS             complement(19588..20616)
                     /locus_tag="VFQ79_17425"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEU0141508.1"
                     /translation="MKIIRTLTIALLSISGMSQAASLSAGDIRAVRAAVEKSLAVMQP
                     SSKQFFQKSGCISCHHQSLGAMAVAAARERGFAVDEELAQYQVKATISIASPHREVLL
                     QAIPAIPVTPIVSSYALVGLAAERYPADETTDALVMELAARQVSDGSWRTDDQRPPLE
                     QSRFTSTTLTARALQLYAPAGRKQEFDKRVAKAKEWLVRTPAHTTQEMTMRLLGLAWT
                     KADRAQVKAAATALLSGQRSDGGWAQLPGLESDAYATGQVLFALHEAGTLKGTDLRYW
                     SGVQFLLRTQEPDGSWYVKSRAMGFQPYFESGYPHGHDQWISSAGAAWATLALATAVE
                     PPRTLSRR"
     gene            complement(20629..21657)
                     /locus_tag="VFQ79_17430"
     CDS             complement(20629..21657)
                     /locus_tag="VFQ79_17430"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0141509.1"
                     /translation="MKLMKSYWLLLCAAVSILPVSARNGNGDGAIFQAIRNNDVLLVH
                     KLIEDGALAVQARPDGTTPLMHAALYSNVPCMRLLMEHGADPNAANAAGATALMWSVH
                     DPAKVALLLDKGADVKARTKAGRTALLIAARQGGSGGLIRMLLAKGADPRDKDEMGGT
                     ALMLAAEAGDLEVVRLLVEKGVEVNAQAHPAFGAPRFGNLMPDFVKAHSGEPPAGPTA
                     LTLAVAADNADVVRFLLEHGAKVNVRALGGFNPLLVSARHKQTTIVRMLLAAGADPNS
                     TEYRGATPLIAAAASDDFSTETIALLLDAGADPRAKDNSGQTALDWAMKRGETAVTGM
                     LRKAVEVQ"
BASE COUNT         4403 a         6799 c         6467 g         4214 t
ORIGIN      
        1 cgcggcggtt cgctgcaaag caacgcctgc gcctcgcgca cgcgcacgac ctgatcattc
       61 cccacccgca tgtagcggtg ctgtcctcca ctcgcgctct cgaagcgagt gaagccgtcc
      121 ggttccacga cgatgcgccc cggaccggat agtgcgaaat aatcgcggtt gggacgaacg
      181 gcatataaca cgctggtgag atcgtaggtc ggctgatcgt tcgtgagtcc gcggtagtat
      241 tggtaggctt ccaccagcgg atgatgatcg acgtagccgt aatcctctct catgctcact
      301 gccggatgat gaatcgcgta cccgatttcc catccgctcg ctacgatggg agtcggccac
      361 tccgcaaata ctttctgcgc cgacgggata tcgcgaacga tattgaactc cttgtaccgc
      421 ttcttggcga gttcctcggt gtacccgcct gccatcgccg aaagcagccg caccttccgg
      481 cgcaccagtt ccgttcctgc caggggtgac atcgcgtccg gcccggaggc cagcaaccgg
      541 gccatgttcg tagagaagcc gaccatcgcg atgacaaccg aattgtccgg ctgtgccgcc
      601 agcgtcttcc gaagcacggc caccgcgtcc ggagcgtcct tgccgctctt gaggtcatgc
      661 ggataccgcg gccctccacc ggatgccgcc gctgttacca ccgcgcgatt gtacttgccg
      721 tcctccggcg tcacaccctt ttggacaaca ccgataggaa tgccgccgcg gccgtagaac
      781 gtattcatga ggtcgataaa gggcgcggca tacgggttgt ccttggtcac cgttacggca
      841 agcaattcgc actcgccccg gttctggaga gcgtggatca tgctgagcgc catcgcatcg
      901 tccacatcgt tgcccatatc cgtatcgaag atcaggcgga ccggttccgc cgccgcggga
      961 gttggcattg agcacaacca gaggcaaagc aacagccagc ctacaatgtt tctgcaagtt
     1021 tcgaaatgca cttccatgtc tccattacgt cttcccgagt ggtcttgata tctccaatgg
     1081 cgagccgcaa cacgaaccgg ccgttcagaa cagtgtgcga cagaaacgct tgcccgtctg
     1141 cattaatgcg gtcgagcagc aggcgatttt gctcgtcgct tcccttgaac cggaagcaca
     1201 ctagtgaaaa cggcacggga gcggcgacct cgaagcgggg gtcggcgtcg atggcgcagg
     1261 ccagttcctt cgcccacgcg atgtgattcc ggaggatttc tataatgccc tcgcgtccga
     1321 aattgcggag cacgaaccac agcttcaaag aacggaagcg gcgccccaat tgaacgccgt
     1381 agtccatgta gttcaccacg cgctcgtctt ccgccgtgcg caaatattcc gccaccaggg
     1441 agaacgcgcg ccgcagaata tccggcttcc gtgtgtagaa gacgcttgcg tcctggggtg
     1501 tgaagagcca tttgtgcggg ttcatgacca gcgaatgggc acgggcggcg ccgtccagaa
     1561 tgtgccgatg ttccggaacc agcgccgccg taccaccata agcgccgtcg atgtgcagcc
     1621 acatgccatg ttcttcggcg aggggaacga tttcggaaac cgggtctacg ctggttgtcg
     1681 aggtagtgcc cacggtcgcg acaacgcaga acggtttttt gctggcgcga acatcttcct
     1741 cgatggcggc tttcagcagg tccgttcgca ttcgaaaccg gtcgtccgcc gcgatctttc
     1801 gaacgttctt ttgtccgatg ccgatcgcga tcgctccctt ctccaccgaa gaatgcgcct
     1861 gttcggagca atacagcacc aggttctgcg acccgccccg ctcgcgtgct tccggatctg
     1921 ccatttcgcg cgcggcggcg atggcgtgca tagtgctgat cgacgcggtg tcgtagatga
     1981 ttccgaaaaa ctcctccggc agcccgagcc attgccggag ccagcccagc gtcacctgct
     2041 ccaactccgt tgcggcaggg caggacttcc agagcatgcc gttcacgttc aacgcggaca
     2101 tcagcatttc gccgagaata ccggggtggg aagcggaaac ggaaaaatac ccgaaaaaac
     2161 gcggatgatt ccaatgatta acggcgggaa ttacaagatt tcgaaaatct tcgagaattc
     2221 gtccggcggg ttctccctgt tccggaccgg atttcggaag cgaacgcacc aggctgcccg
     2281 gctccataat gggcatcacg cggtagtctc gaattccctc gagataatcg gcgatccaat
     2341 ccaccacttc atgcgcggtc ttgcgaaact cctccggggg catatcaaaa ctcatttggc
     2401 gggctcccgg tgggcggtgc gcagggtcac agccgcggtg gctttcatct tttccgcttc
     2461 cggcggcggc acggtccact cggaggtgaa ccggccttct gcaataacgt tatcgagcga
     2521 acctttaaac aaaatgtagt ctccgaaact tagtaacaat cgatacggcg gcggcataaa
     2581 tcgtaatccg gctcgttgag cgcggtccgc gcccgtccac cagggcagcc ccgaattcac
     2641 aagcgcgtaa tgctcaccca gcggcatgac aaagaccagg ccgaaatcgg cggccccggc
     2701 gttgagtgcc atgggagcat cgccggcaag acgcgcgatc aggctattcg tttcacgcgt
     2761 gccgaacagt acgaggttcg agcctttaag atcagactcc ccgacgtctt tgtccgctac
     2821 ggtgcgaaag ttcaccagta gtttcagccg cggcgtggac cattcggcag cctcttctgc
     2881 ctgccgcctg cgccggttca actcttccgg tgagggagaa tccgcggtgc cataaacata
     2941 gacgtgccgc gccgcgaccg cctcaccgat agggccttcc gagccgggac gcttctcgcc
     3001 gggcggcggt gtatacctgc ccggctgcca ccccttttcc gctttccgga acgacagctt
     3061 ttccgtgcct ttcagtttca ggactgcgcc gtcaatcgtg accgacacgg cgcgtcccac
     3121 cgcgaaagaa gaatgcccgg caggagtaag tgtgaaacct ttcacgccgc tggtttcgac
     3181 aacaagctcg ttgcggttct ggaaacgcgc ggagatcttc gccagatctc caggtgtgag
     3241 gccgtcaagc tgtacccagt aagcggagtt gtacttgtaa ttgcgggtcg cgaatcggac
     3301 ttcgtccggg aagcggttgc ggcggaactg tgagaaccag tcgaaaatgg cgccgtcttt
     3361 atacgccaga tcccaggaat tatgccgcac tccgggatac tccgtgtatt gcacgtttac
     3421 gccgatgcgc agcagttcat ggttccactt ccgcgactgc tccacgggaa ccgccgggtc
     3481 ggcatcccca tgaaacaagt gaacgggtat gttcagaaga ttcggcgcga ggtcgagcga
     3541 gccttccgga acggcggcac agaccggcgc gatggcggcc cagatatcgg gccgggtcag
     3601 ggcgagccag aacgttccgc caccacccat cgaaagaccc gtcaggtaaa tccgatcttc
     3661 atcaatcggg aagcgccttt tcacgtcggc cagcacgtcg tagacatcct gctcggggat
     3721 gccctggtaa cccatggtgc cgcgcgaata cggagaggca acaaggaagt cgacatcccg
     3781 gagcgccgga aagtaacggg tggcctcggg gtctgtctcg ccggcccggt tccctttgcc
     3841 aaacacgcgc cgcaagttca ggcggtggtt ggaccatgcc ccatggaggc tgaccaccag
     3901 cggatacttc ttcgcggcgt cgaagttctt cggcagatac aaggcgtaag gttgatcgga
     3961 gtcgtcaata ccggaaaaga acgtaagcac ctgcggtccg gcagggtaca cctgcgcgcg
     4021 cagagccaga gcacagaata gaaaaagaaa tagcttgaca gaccgctcgc tcacgctcgc
     4081 ggctcggaag ccggcaccga gccgcgagcg tgagcgagcg gttcctacac taaacatcct
     4141 tgcactcctt ttgtacagcg ggcagccatg cctgaaacca ggcgcggaat tccccggccc
     4201 gttgtacatt ttccgggcca agcgggaccg ctggaggacc ggctttcaag ccccgcgcca
     4261 cggcggcttc ccgaatgcca accggtgagg gaaacgaatc gatccacccc aaaagttcat
     4321 tgagccgggc ttccagacgt gcgacggcat ccggcgcgcc agccgcgatt gcacggtcca
     4381 gcgccaccat caactccggc accacacacg cgatctccga gatagcgccg gcggctccgt
     4441 gcgagcgcgc cccgacgtat gcggcgtcgc atccggcgat gcaaggaaac ccggcattcg
     4501 aatcgaatgc gccccagtgc gcgcttgcat cgtggattcc cgcaaaccgg ccggtcgcga
     4561 gcagccgggc tgcggttggg ggagcgattt cactggtgaa agaaggcaca ttcgagagat
     4621 aaacgggcgg gggcgcgcca cacagatcca tgaattcaag ataaaaccgc tcgatctcgg
     4681 gttgttcgta gcgaaagaaa tagggcggca tcagcaataa gccggcaaca cctgcgccgc
     4741 tgatttcctg cgcgatgaaa acagcaccat cgagagtgga gtgtgagcaa tttgcaagta
     4801 ccggcaggcg gcttcgcttg gtggccagcg cggtcacacg cactcgttcc tccgggttaa
     4861 aatgcacgaa ttcactcgcc gggctgagca atgcgattcc atccacaccg ctctcgccga
     4921 gataatccac gaattcgagc atggccgcga ggtcgatctc atggccactc gcgcgccggg
     4981 gagtaacggg agcagcatag atcccctgaa acgaagccat gtaccatttg ggataacact
     5041 gtcttcgata gcttatcaac cttcacaagt ttcgatatgc tggtcgggac caaaagcact
     5101 tcagacaggg aggaaaattt atggacgtct cacgccggtc ctttgtcgcc ggatgcgccg
     5161 gagtggcttc ggccggcgct cagacctcgt caccccggcc tgtccgcatc ggcgtggtgg
     5221 gcggcggctt cgggtccagt tttcaatggc atctgcagcc ggactgcaag gtggaagcgg
     5281 tgtgcgacat caggcccgac cggctgcaac gcctgtcgga agtctaccgc tgcggcgccg
     5341 cctttacgaa ctttcgggaa atgctggcga aagccaagct ggacgcggtg ggagtattca
     5401 cacccgcgcc gctgcacgca tggatggcca tggagagcat gaaagcgggc aaacacgtga
     5461 tcagcgcggt acccgccgga ataacggtgg aagaactgga gcgaatcctt gaaaccgtgc
     5521 gttccacagg catgcgttac atgatggcgg agaccagtta ctaccggccg gagatcatga
     5581 cgtgcatgga aatgcagcgc aagggcgaat tcggcacgat tttttactcc gaggccgagt
     5641 atcaccacga gggcctgatt cccctcatgt tcgataagat cgggaacccc acgtggcgtt
     5701 atggcttccc acccatgctc tatccgacac actgcaccgg aatggtggtt cccgtcaccg
     5761 gcgagcgtct gacaagcgtt caggccgttg gctggggcga cggtcacgaa gttctgcaaa
     5821 ccaatatgta cacgaacccg ttctggacta cgacgggatt cttccgcacg tcgaaaggcc
     5881 actcgtcgcg catcagcgtc ttctggcacg tcgcggcagg tggaaccgaa cgcgcccaat
     5941 tctacgggga ccgcttgtcg tacatcatgc agaggcccga aggatcgccg aatactttaa
     6001 tcaaaatatc gaaggacggg aagacggtga tcgacgcgaa cggatatccc gagggcgacg
     6061 tgaagatcga ggctttcaaa cagccggatc acctggaaaa gctgccgcct tcgatgcgcg
     6121 tcaagacagg tcacggcaac tcgcacacgt tcatcacgca ggagttcatc cgcgccatcg
     6181 tggaagaccg gcacccggcc gtgaacatct gggaggcgat agcctacacg ctgcccggta
     6241 ttgtcgcgca ccagtccgcc ctgcgcggcg gtgagccgat gaagatccgc gattacggaa
     6301 caccggcgta gaaaggcccg cttgctcacg cgcgcggctc agtaggttta ttgcgtttcg
     6361 gttttcgcta tgccagcgcg acggccggtt tgccttcgta ctgtatgcgc acttcacgtc
     6421 caccgttcag gatggacagg cggcctgctt cgatcaccag ttcgttgtag cggctgttgg
     6481 cgggtgtagc catcacgcct tcttcatcga actgccgcaa catttgctgg cgctcctcca
     6541 gagtgcccgc ttgggattcg aggcgcacgg cagcccggat aatgaaatcg accgagcgat
     6601 atccataacc caccggcacc agcccgcccc cgcccagatc gacatactgg aaataatcgg
     6661 gactcggttc ggcatagacc gtggcgcccg gaccgtccgg cggacgtacg tagctgtatt
     6721 cgagcccgcg gtattgatcc gagtgaccca gcagtccgcc tttctcccgg ccggagcaat
     6781 acaataccat gccctgcgtg ttggtaccgg gagcatcgtc cgggaatccc agcgcgttct
     6841 gcacgttcag agaggcaccg ttgttccaca gcacccgcgc gtcggtccac aggaatcctt
     6901 cgtttccgtt cggatagcga tcctttatgc catatacgct gacggaaaca ggaagcaacc
     6961 cggtaatgaa gtgcaccagg tccacgtaat gacagccgat gtaagtgaac gcgtcggagt
     7021 tctcgaccgt gcaccagttc tgaaaattcg aatggcggta ataccacttt tccagcaggc
     7081 acgccgtgcc gagccggaat tcgccgaacc aaccgtcgcg ataacggcgg cgggccatca
     7141 ggctgcggtc gtcgaatcgc ttgtgatact cgattcccac cagcaggccg cggcgcgacg
     7201 cttcctgctc gatctcggtg gcctccgccg cggtcagcac cagcggtttc accgtcagca
     7261 catgctggtc gtgccggagc gcctccatga tgacggcatg gtgcaactga tccggcacgg
     7321 ctacggctac gatgtttcgc ggcggaaggc cggcgatgac ctgcttgtag agatccaggt
     7381 gaactcccgt atcggagagg tccgggaacg gccggaacga ctggcctgga aaagccttgc
     7441 tgatgttttc cgaatccacc agcgacttga gaaatctcgc ttccgtcgcg cagacgctga
     7501 tttctccaat cacaccctga cgctgcaagt gataaagaga cggcagaatc tgatcgtgcg
     7561 cgatcatccc cccgccgata agagcaacct ggagcgggac catcagacag cggcctcctg
     7621 cttgcgcgcg gccagcgctt cggagaacgc ttcgctaaat gccgcggcgc tctcgcggat
     7681 ggcctcttcg gtgatgacca gcggcgggca gattttcacc gtgcctccac cgaacccgac
     7741 gggcgagaac atgagaacac ccttggcgat ggaccgctcg acaatgtccc acgcgagatc
     7801 gccatcgggc tctttcgtac ccggcttcac gcaactcagg cccacgacca gccccttgcc
     7861 atccacactt ccgatttcag gatgtttggc tttcaggtca tttagcagcg agagaagcaa
     7921 agtgccttgc ttcctcgcgt tctcaaccag gttttctctt tcgacaatct cgatggacgc
     7981 cagcgccgcg gcacagcaaa cgggatttcc tgtgtgtgtg gaggtcatac tgcccggcgg
     8041 gtgcaaatcc ataatgtccg gccttcccgc caccgccgag atgggcagcg aactggaaat
     8101 acccttgccg aaggtcgtca ggtccggcac gattccatag tgctcgaagc cccacatggt
     8161 tccggtgcgc ccgaagccgg cctgcacttc atcacagacc aacagtgctc cgttggcatc
     8221 gcaccacttc cggagccgct tcatatattc gggcggggcg aacgaagcac tgccgccctg
     8281 atatgtttcg aggacaacac cggccacgtc cgccggcttg atgcccgctt tggaaaggca
     8341 ctcttcgaaa aattcgaaag acgtgttctc ggtgcggaag ccgtcgggga aaggaatctg
     8401 aacgaaaccc ggatcgaggt ttacaatcca atccttcagc gccggaatac cgccggcctg
     8461 ctgcgagccg agggtccgtc cgtggaaggc cttgtcgtag gaaacgatga cgttcttcga
     8521 acgtccgcct accttaaccc cgtttgtgcg gcagagtttg atggcacatt ccacagcttc
     8581 cgatccggta gtgagcagga atactttttt caacggatcc ggcagcagcg atgcgatctt
     8641 ttccaccagg cgcgcgcgga tttcgctggg gaagcagtag ttcgtcagaa gcttcgaggc
     8701 agcctgtccc gacacggctt cgataatctc ccggcgtccg tgtccggcgt tcgtgatcaa
     8761 gacacccgac gaccagtcaa tccagcagtt cccatgtgca tcgaacacct gaaatccttc
     8821 cgcgtggtcc cagacgatgg gcggttggcc tcgcatcgct accggctcat actggtgtag
     8881 cttctcaagc accggaaccg atgcgggcac cggcaactcc gtcacaattc gccttagggc
     8941 tgtctctatt ctggggaccc tgcgcggggt caactcgtat tctcttgcca tttcttgcaa
     9001 ttgtcccaca agcttcaggc ccacaggtat tttttaccgg agtgcagccc ggttcacgcc
     9061 ggcttttcaa agagatccgt ctggaagcca aaatgccata cgtacgtcgc catagcggga
     9121 gttttgctgt tttggaccgc gaatcgccca cctaagggga acggatgtct gggagggcga
     9181 gtggcgtgag tagcgccggt caggcggccg tgggatgcgg agctgggttt ttaccgtttc
     9241 ccctgcttgt gaaagcctcg tagggcgtga aagcgctctt cagaacagcg cgagactttc
     9301 aacgcaaccg gaacaatttc acggcccgcc ctctggcgcc tatgcagggg cacagattat
     9361 catccaacaa tatgtccgac tccgataaga acgagcggcc gccgcggacg gtgctgccgg
     9421 gaagacctta tccgttaggc gccacctggg acggcgccgg taccaacttc gccttgtatt
     9481 ctcacagcgg caccgccgtc gatctctgcc tgttcgacga tcgaggccag gaggaacaaa
     9541 tccctcttcg ggagcaaacg aacttcgtct ggcactgtta tctgccggcg gcgccccccg
     9601 gacaactcta tggttatcgc gtgcatggac cttatgagcc gaaacgcggc ctccgattca
     9661 atcccgcaaa actgctgctc gatccttatg ccaccgctgt ttcgggagag gtgaattggg
     9721 aagcgccgct cttcggctac caactgggtc acccggacgc cgacctttcc tgcgattccg
     9781 gtgacagcgc atggggcatg cccaagggcg ccgtcgtcag cccgtttttc gattgggaga
     9841 acgaccggcc tctccggact ccgttaaacg actcggtgat ctatgaggtg cacgtaaaag
     9901 gattcaccat tcagcacccc gacatcgctc aggagttgcg tggcacctac gcaggcttgg
     9961 gatcgccgcc cgcggttcag tacctgaaaa aactggggat cacggccgtc gagttgatgc
    10021 cgatacacga catgctggac gacaagcacc tggttgacca aggactgaag aactattggg
    10081 ggtataacac caccaatttc ttctcccctg cggcccgcta tgccagcgca ggcgacaaag
    10141 gcggccaggt ggccgagttc aagtcgatgg tgaaggcgct gcatcgcgaa ggcatagagg
    10201 tgatcctcga cgtcgtgtac aaccacacgt ccgaaggaaa ccatctgggc ccgacgctga
    10261 gctttcgcgg tatcgacaac ctcacttact atcggacgaa cgacgatgcg cgctactaca
    10321 tggattacac aggaacggga aatagcctga acgtgcgtca tccacaggtc ctcaaactca
    10381 tcatggatag cctgcggtat tgggtggagg agatgcacgt cgacggtttc cgtttcgatc
    10441 tcgcagccac tctggcgcgg gaactgcacg atgtagaccg cctgtccgcc ttcttcgaca
    10501 tcatccacca ggatccggtc ctatcgcagg tgaaactgat cgccgagcca tgggacgtgg
    10561 gcgagggagg ttaccaggtt ggaaacttcc cgatcctctg ggccgagtgg aatggaaagt
    10621 atcgcgacgt ggttcgccgc tattggaaaa gcgaggacgg cacgttagcc gagctgggct
    10681 accggctgac cgggagcagc gacctgtacc aaagagacgg gagaaggcca tcggcgagca
    10741 tcaatttcgt taccgcgcac gatggcttca ctctgaacga cctggtcagc tacaacgaaa
    10801 agcacaacga ggccaacggc gaagagaatc gcgacggctc aaacgacaac cattcgtgga
    10861 acatgggggt ggagggcccg accagcgatc ccgaaatcat cgaattgcgc gagcggcaga
    10921 aacggaatct gctcgccacg ctgatgcttt ctcagggcgt gccgatgatt tgcggcggcg
    10981 acgagatcag ccgcacgcaa cgcggcaaca acaacgccta ttgccaggac aacgaaatca
    11041 gttggcttga ctggacgccg gaggccggta agcgggcgct gtttgaattc acccgcgaac
    11101 tcatccggct ccggctggaa catcccaacc tgcaccggcg caaatttttc caggaccgcg
    11161 agatccgcgg aaccatagag aaggacgtga gctggttccg gcccgacggt gaagagatga
    11221 cggacgagga gtggtcctcc gggtgggtgc gatgcatcgg cgtgcgcttg aacggtgaga
    11281 cactcaacga ctccgattcc aagggcgctc cgctacacga cgataccttc atgctgctgc
    11341 tcaacccgca tcacgaaccg atcgattttt atttacccga acccgacaag caaacacact
    11401 gggaaatgct cgtcaatacg attcagccgg ggttctacca gaatcagtgc attaaatccg
    11461 gtgagcccgt caacctcgcg cggcaaacac tggtcgtttt gcaacaggtg gcgccacacg
    11521 ttcccacggt gccgtaacgg cccgcgaggg atagcgcacg ctgtatagtg tgctacatgt
    11581 gtcgagctat ttctgttttg ccgctcgtgt taacagtggc gctttgccaa tctcccgatc
    11641 gcgaagcgct gccgcaggag tggggctacc gcccggcgga tggcgccacc gtgccgttga
    11701 accctcccgc actttcgtgg gtgccggaga agggagcagc gggttatacg gtccagtggg
    11761 cgaacaatcc ggaattcgcg gaccctgcga cggtgcccaa tctgcgctgg accgtttaca
    11821 cgcaccatca accgctgaag cccggcacct ggtggtggcg ctaccgcatc gcctcggaag
    11881 aagaacgcca atcgtcatgg agccgtgcgc ggcagttcat cattcccgcc gaggccgccg
    11941 tctttcccaa gcccacgatg gaggacctgc gccggcggat tcccaccgga caccctcgcc
    12001 tgttcgtgcg cgcggaggat ctgccgaaat ggaaacaatg ggcggcgggc ggaggaaaag
    12061 agccttacgg cagactgctc gcgcgcgccg acgcgctgct gcgggcagag cccacgccgg
    12121 aacccaccgt tcgcgcaaat ccgtatgacg cagccacacg ccagtattgg tggtcgaatc
    12181 gcgtcgagac cattgaggcg ctccaggaag cggagatcct cgccttcgcc tggcttctga
    12241 ccgggaattc gaaatacggc gagcaggcaa agcgcttcac gttgaagctg gcggaatggg
    12301 atccggatgg acccacccgg ttctccttga attgtgaagc cgccaaaccg ctggtccacc
    12361 ggttggcccg ggcctacgac tgggcctggc cgcttttcac tcccgaggag cgctcgaaaa
    12421 ttcgcgccgt gctgctgcgc cgcgccaggg atgcatggaa cagcggcgaa gtgaagcagg
    12481 gtggtggaca tctcgccgag ccctacagca gccacggcaa ccggacctgg cacaagctgg
    12541 cggaaaacgc catcgccacg ctcggcgaaa cgccggaatc ggaggagttc ctcgaatacg
    12601 cgatggccaa gttcttcggc gcctatcccg tctggtccga cgacgacggc ggctggcacg
    12661 aaggcctgaa ctatctcggc ggctacatgt cgaaaacacg ctggtggatc gacattgcgc
    12721 gaaccgcgat gaacatcgat gcctttcaaa agccgttttt cacgcgcatc ggcgattatg
    12781 cgatgtactc ggctcctccc ggaacgccca acttgggttt tggcgatctg gctatcggac
    12841 cggtctcgcg cggatggtcg tttctgcatt actacgtccg caacaccggc aacgcgcatt
    12901 gggcgtggtg gctgcagcaa tgggatgtgg gcgagaccaa tgaggagccc gtactcgatt
    12961 ttctctggag cgccgcgccc agacctaacc cccaggcgcc ctccggagtg ccgccgtcga
    13021 aactattcga gggaaccggc gtcgcaatcc tgaactcgaa cctgctcgat ggccgcgaga
    13081 acgtgcagct tcgattcaaa tccagtccca tggggaggtg gagtcacgga ctggacccgc
    13141 agaacagttt cacgctgaat gcctacgggt ccgcgctgct ggtgaacaat gtttatcgcg
    13201 acctgtatgg cagtccgttt cactccaaat gggtctggag cacacgcgcg caaaacgccg
    13261 tgcttattaa cggcgagggg cagcggcctc gctcgcccgc tcacgacggc cgcatcgtga
    13321 aagccgaatt ccagccgggc ctggactatg tctcgggcga agcggcgccg gcttacgccc
    13381 agaacctgaa gcgcgcacgg cggcacatcg tgttcgtgaa accggacgtg gcctttattg
    13441 ccgacgagat ccaggcggcg aaacccggca cataccaatg gatgcttcac gcaccgcagg
    13501 ccttccaggt ggacgaatcg caacagcggc ttgtgctgga ccggggcgcg gcgggagtca
    13561 tcgtcgaata tgcggctccg cagccgctca aactgcggca atggaccggc tatgatcccg
    13621 aacccgacgc cgagtatctc gcatcggtga agcggccgcc gattcctccg cagtggcacg
    13681 tggaagcaag cgcggacaaa cccactgacg atgtgttcac cgtcaccgta ttacgcgtct
    13741 tccggcaggg acaaaccccg ggatcgcaga tgcgtgtctc cagggacggc ggcacgctgc
    13801 gaatcgcggc gggcgatgtc gaagcagcgt tcgaacagcg gcgcattgtg gcgcgcaaag
    13861 gcgatcgccg gtggacgatc gcactaccgg aatagtgccc ctcacggctc gcgccggatc
    13921 accagcgcca caccgccctg atcgtacgtg tccaggtaca ggtactggcc ctgttgcagc
    13981 agcctgacga gatagatcgc gccgtcgtcg ccttgcagaa agagcacata ccggtcgccc
    14041 tcgatttgct gaacgctcac cagccgtccc cattcccgcg gcaggaatgc gggctggccg
    14101 gattcgctcc ggctcacacc tatgttctgc aaagactcga tcttggccgg ttttactttg
    14161 gattgctgct ggccctgcga cgccgccggg cggaatggaa gtaagccaac cagcagcatt
    14221 atgcagacta tgaatgtccg tttcatgtga ttctcctcca tgagcgctga cgctcgccat
    14281 tcggagtttg ttgcggaaat tgccaggact gctaagctgg aaccgtactc atgacatctc
    14341 gaagggcgtt tttatccgtg gccggctcgg cacttcccct ggccgccaaa cagaagttct
    14401 ccgggccgct tggcctcgaa atctacagtc tccggcgcca ggcgcagaaa gatcttcccg
    14461 gcactcttgc gatgattcgt gagatgggct ttaaggagtt ggaggtcgga ggatattacg
    14521 ggcgcacggt ttccgagttc aaacgcatgc tggcagccaa cggactggcg gccacatcga
    14581 tgatggccgg gtgggaccag ctttcgaact ccaccggcaa ggtggcggaa gaagcacgta
    14641 ccctcggctc cggatacgta gtctgcgggt ccatacccca caagaagcct ttctccgcgg
    14701 acgacgctca tcgcgccgca gaccatttca acaaatgggg ggaagcgctg aagagcgccg
    14761 gcctccaatt ctgctatcac gcgcacggct ttgaattcgg ggccagcccc gacggcacgt
    14821 tgttcgacac catggcgaag cgcatggacc cgaaggtggc taacttccag atggatgtgt
    14881 tctggatcgc cttcggcaag gaagatcccg caaagcttct ggagcgctac gcgggccggt
    14941 tcccgctgat gcatctgaaa gacatccgca aaggggaagc gaaaacgggc aaccccggtg
    15001 atgtggtgga ggaagccagc gtcccgcttg gcacgggcga aatcgattgg cccagcgtgc
    15061 tgagcgcggc gcgtaaaacc ggtgtccgcc gctactacat cgaagaggaa cacccggacg
    15121 cggtgaaaca gatcccaaag acgctgcgtt atctcgagaa cattcgcttc taaacgcgaa
    15181 agaccgcttg ctggcgcgcg cggctcagaa tcgactccgc gccgttacag caacttacag
    15241 agtggtggcc ttggcagttt ttcaacaagc ttctaaacca tcaggcgtag cagcggactt
    15301 cgaagatccg cacttcttcc gcgccgttcg tcttcgtagc ggtaatccgc acggagagtg
    15361 cgtcgaccgg ttcgaacgtg acgcgattca agcgctggtg gttgcctgtt accgtggcga
    15421 ccgtatgctg gcggccgtcg gcgccggtca cggtcaccgt gtaatcgcga accgtctccg
    15481 gctgcggcgc tcgcacaatg gcgcgtgaaa tcgagtccga agccgagagc gtcagctccc
    15541 gctggaagcc ggtatcgaac ttcagcacca cctcgcgcag ccgttgcgcc tggcgccagg
    15601 ataactcgat ccaagcttcc cgggagccca catttgcagc ccagtgatgc acttcgcccg
    15661 gctttcccgt cctcttattc gccgggatgt cgcggtcgat tccatccgtc accagcgcgg
    15721 ccttggcctc gcccacttcg cctgaagctt tgatggccgc ggttcgcgcc agatcgcgtg
    15781 tatcgcgatt ggagaccgta cggatagact ggtcctggcg caagagcaac tgctggaggc
    15841 cactgacaag ttgacggtcg cgcgcaagct gccgcggtgt aacgccacgc tcgcgacaga
    15901 gtgcagccgc tgttcccacc gcctggccaa tggatgcgca ggtcgccatt acacgggtgc
    15961 tggtgaacgc cgcatgcgaa gcgctgatgt tccgccccgc catcatgagg ttcgtgatgt
    16021 tccggctgta gagcgcgcgc agcggaatat cgaaaacctc atcggtgtga atgacggtgt
    16081 tgggcggcag atccgggcgg tcaaaacctc cgggcggatg atcgtccatc ggccagccgc
    16141 ccatcgccac ggcatcgggc catgttcccc gcatcaggtc cccttgcgta aggatcacgt
    16201 cgcctaccag gcgccggctg ccgcgcttgc ccggcatcat gccaacccat tccagcgccc
    16261 aatgcgcact gtccggataa tcgccgctgt tcttgatgta atcccagacg ccggtcacaa
    16321 tcgagagcaa ctcgaaccgg atgcgttcgt tgtcgcgaat cgtatccttg tcgccgcccc
    16381 attcaatcca ccagtagcca tactcccagt tctcgggact caccttgcga aagcggagat
    16441 gctctttcgt aaccttccgt gcccatttgg gcggcgtgta aggcatcggt ctgcgatgca
    16501 agcgcgaggt gaacagaatg ctgctgccga gggtttcctg gtcggctgtt tcgggagcca
    16561 gcgattcgtt gaactcactc cgtgtctccc ggccggtccg gtactcggct cccgcttcga
    16621 gaccgagacg gctgtcgccg gtggcatcga catacagccc ggccttgatg cggtacaaat
    16681 gctcgctctt gtcgcaacgc gccattacct gctcgatacg gctgccttgc atggtggctg
    16741 aatacagaat ggtttcgagc agcaacgtga tgttcggctc gctgacaact ttgtcgtaaa
    16801 gcaacaggtc ccaaagctcc cagcagcgct gcgggttgtt tgccgcatcg tccagccgga
    16861 actcctcaat cagcccgccc tcgcgccagc ccgggcggcc tttgtggctg ttcgcgccga
    16921 cgacgtgcat tttcacttcg ctggaggagt tgccacccag ccgggaacgg tcctgtacca
    16981 gcacgacttt tgcgccattg cggaccgccg ccaccgcggc gcacacgccc gcgaggccgc
    17041 ctccggcgat gaaaacgtcc gtttccagat cgacgacggc catgttgggt tccgcctccg
    17101 ccttccggcg ctgcgggtct ggtggcgcga tccggtcgaa ctcgcggcga agccgttcgc
    17161 tcacagccgg cgccggaccc tgtgggatct ggtccgccag caccaccaag gagtacccac
    17221 ccagtcctaa cccgctgagc aaatcacgtc gacgcatttc ccttcacccc tccagataac
    17281 ggccgatcgc tttcgcgagg tcgattccaa gagcccgcgc ggatgcggcc gtcactgccg
    17341 ttccgccggc cgtcgcgtac gggatctcca gggtggaagc aaaccggatg cccggctgct
    17401 ccgcggccca cataccaaag ctcttgaggc ctttggtgtt tgcatccgtg ttccatcctt
    17461 cgccgtaagg caagttattc tccgcacggt aaacgagtgg accggattgc acggactcga
    17521 gtattttcga aaaacgcacc gcctcccgcc agatctccgg gtggcgcgag ccgacgaagt
    17581 agatctcctc gttacctttg ccgctgatgt acgggcaatg cagatccatt gcaaatcgca
    17641 atcgcccgtc cgaccatttc ggcgtcaact cgcgaagggc ctttacggac gggtgtatgc
    17701 tctctccgtc gtaatcccgg ttgtgatcat gcgggcggcg attcttgccc tggtcgccgt
    17761 cttccacacc gtccttatcc ataaacggca caaccaggaa ctccacgttt tcgcgcagcc
    17821 aggtcccggc ggtgtcctcc acgatgcccg cgagaattcc ttccaggctg tagctggcga
    17881 gcgattcgca ggcatgatgg cgcgccgtca gaatcgcgcg cattttggga ttctcgaaat
    17941 ttcccacacg caacatctcg atatcgcgcc ctttccggct tttcgccaga acaccggcct
    18001 tgagatgcgg gcttccgcga tggcggtcga gaaaacgtac gagatcggct tcgaagtaag
    18061 gaatgttgac ggagaaccgc acctcctccg cgcgtgccgg gaacgtgtac gtgaaggatg
    18121 cctggtggat ggaatccgcg ccaagccact gccaggtgtg tccgcggtcg agactgaccg
    18181 ccggaccgag tgccgatagc ggcgcccggc caggaaatcg aaacgtcatt ttgcggcccg
    18241 ccgcgccccg cacacggaaa gcccagtaga accagtcgcc ttccgtatcg cggaggtcct
    18301 ggcggacgga gacggtatcg ccatccacgg cgtcgacgat gatgttgcct ccagggatgt
    18361 catcatcgat gcgcacggcg gccggaactg cgcccatcac cacagaagga atgacactaa
    18421 acaatgttcg gcgtgtgagc atgtcggcca gtgaacaacc attctatccg ctccgcgggc
    18481 gcaccttcgg gcatatggga aaatggttgt atctgaagca ctcatgcacg agctttccat
    18541 cgccaccagc attgtggaac tcgccgaaga agaagccgag cgccggggcg ttcgcgtcgt
    18601 tgcggtccac ctgacgctgg gtcaactctc cggcgtggta acggaggcgc tggcgggctc
    18661 ttacgatatt gcaaccgcgg gtacgccgct cgaaggatcc cggctcgtca ttaccgaagc
    18721 tccggtagtc gtcttttgcc ctgcctgccg cgaacggcgc actcttcctt cggtgcagtt
    18781 gtttcaatgt ccgcagtgcg gaactccgac ggcggacgtg atcgaaggcc gggaattgca
    18841 ggttacggcg ctggaggtgg agtgatgaaa ccggagccgc ggctggtgga agtgcggcgc
    18901 aacgtgctga agcacaatga tcttgtagcg cgggaactcc gcgcacgttt ccagcgggaa
    18961 ggcatctcgg ttgtcagcct cgtgtccagc cctggctcgg gaaagacgcg ttttctggag
    19021 agtacgttgt cccggatgaa tggccgtcac cgggtcgcag ccctggttgg cgacctggcg
    19081 acagagaacg atgccgttcg tctggcgcgc agtggggcgc ccgtgaaaca gattgtcacc
    19141 ggcacggtct gccatctgga cgccgggatg atccgaaatt ccctcgaaga atggcatctg
    19201 gcggatgtga actatctgtt tatcgagaat gtcggcaacc tggtttgccc cgcttcttac
    19261 gatttgggcg aagacgtgcg cgcagtgctg atgtcggtga cggaaggcga agataagccg
    19321 ctgaagtatc cgacgatctt caacggcgcc gacgtcgcgg tgatcacgaa gatggatttg
    19381 tgtgaagccg cggaattcga tctcgaagcc gcaagagcga acatccaggc cgtgcgcccc
    19441 gggctccgtg tactgtgcgt ttcggcaaag acgggtgaag gaatggaaga gtggatggaa
    19501 tacatcgaat cgctcacgcc cgcgcagaaa taaactgcgc tcccggctcc atcctcgaag
    19561 ccgggagcgt ccaacgcgcc tgttgtttta ccgccgcgag agggtacgcg gcggttctac
    19621 ggccgtggcc agcgccaggg tagcccaggc agcgcccgct gaagagatcc attggtcgtg
    19681 gccgtgcgga tacccacttt cgaaataggg ctggaacccc atggcgcggc tcttgacata
    19741 ccacgatcca tccggctcct gcgtacggag caggaactgc actccgctcc agtaccgcag
    19801 gtccgtaccc tttaaggtgc ccgcttcatg caatgcgaag agcacctgac cggtggcata
    19861 ggcatcgctt tcgagtcccg gcagttgggc ccagccgcca tcggagcgct ggccggaaag
    19921 cagcgcagta gcggcggcct ttacctgtgc ccggtccgcc ttcgtccagg ccagtcccag
    19981 aaggcgcatc gtcatctcct gcgtggtatg cgccggcgtg cggaccagcc attcttttgc
    20041 cttcgcgact cttttgtcga actcctgctt ccgtccggca ggcgcataaa gctggagcgc
    20101 gcgcgccgtc agcgtcgtgg aggtaaagcg gctctgttca agaggcggtc tctgatcatc
    20161 cgtacgccag gacccgtcgg acacctgccg cgcagccaat tccatcacca gggcgtccgt
    20221 tgtctcatcg gcgggatacc gttcggcggc taaaccgacc agcgcatagc tgctcacgat
    20281 cggcgtcacc ggaatcgccg gaatcgcctg caacagcacc tcgcgatgag gcgacgcgat
    20341 ggagattgtg gccttgacct gatattgcgc cagctcttcg tcgaccgcga acccgcgctc
    20401 cctcgcagcc gccaccgcca tcgctccgag cgattggtga tggcacgaaa tgcatcctga
    20461 tttttggaag aactgcttgc tcgacggctg catcacggcc aggctctttt ccactgccgc
    20521 tcgcacggct cttatatcgc ccgcactcag gctggcggct tgcgacatcc cggagatgct
    20581 gagtaaagca attgtcagtg ttcgaatgat tttcatggcc gatctcctct actgcacctc
    20641 tactgctttc cggagcattc ccgtcaccgc cgtttcgccc cgtttcatcg cccaatcaag
    20701 cgctgtttgt ccgctgttgt ccttcgcacg cggatcggct ccggcatcca gcagcagcgc
    20761 gatcgtctcc gtgctgaagt cgtccgatgc cgccgccgca atcaacgggg tagctccacg
    20821 gtactccgtc gagttcgggt cggctccggc agccagcaac atgcggacga tcgtggtttg
    20881 cttatgtctc gcacttacga gcagggggtt gaagccgccc agtgcgcgca cgttcacctt
    20941 cgccccgtgc tccaacagga accgcacaac atcggcgttg tcagccgcca ccgcaagcgt
    21001 cagcgcggta gggccggcgg gcggctctcc ggagtgagcc tttacaaaat cgggcatgag
    21061 attgccgaaa cgtggcgcgc cgaaagccgg atgcgcttgg gcattgacct ccacaccctt
    21121 ctctacaagc agcctcacta cttccagatc gccggcttcg gcggccagca tcagggcggt
    21181 accgcccatc tcgtctttgt ccctcgggtc cgcacctttc gccaggagca tgcgaatcaa
    21241 accaccactg cccccctgac gcgcggcaat cagaagcgct gtcctgcccg ctttggttct
    21301 ggccttgaca tccgctccct tgtcgagcag caaagccact ttcgccggat cgtgcacgga
    21361 ccacatcagg gcggtggcgc cggcggcgtt cgccgcattg gggtccgcgc cgtgttccat
    21421 caacagccgc atgcatggaa cgttcgagta aagggcggca tgcatcagcg gcgtcgttcc
    21481 gtccgggcgt gcctggacgg ccaatgcgcc atcttcgatc agcttgtgga cgagcaacac
    21541 atcgttgtta cgaatagcct ggaagatggc cccgtcgcca ttcccgttgc gggcggacac
    21601 gggcaaaatc gagacggcag cgcacaataa cagccaatac gatttcatga gtttcataag
    21661 cttcctctgg tgtgtggggt cgcgacccgt atcagtcaag aaacgcgcga aaacaaggca
    21721 gagtagctca cggggagaga aggcggcaaa ccgctcgctc acgcgcgcgg ctccgttccg
    21781 ggagcggctg gcttctaacc caagttcgtc acgggaccgt cggttttaac gattttgagg
    21841 ctttggacgg gagcaagtcc tgcgtttgca atcaccactt ctg
//
LOCUS       DASTMK010000405         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_404745, whole genome shotgun sequence.
ACCESSION   DASTMK010000405 DASTMK010000000
VERSION     DASTMK010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404745"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFQ79_17435"
     CDS             complement(<1..859)
                     /locus_tag="VFQ79_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141510.1"
                     /translation="MNRGACRLLGFLLAVCATASLHIAGAQEAADPILRAMREEIERS
                     RQLSIVNLDSPYYIEYSLDDLDSFSASASLGGLIGSRHTRVRLPRVQVRVGSYDFDNT
                     NYIFTDLFGRVGSAGFPLENDLLAMRTFFWLATDQAYKGAVQAIARKRAALQHVTESE
                     VLADLAKAQPTQKILPVRRYTVNQEEWKARVRQLSAIFAGYPAIIESDVEFQAGQSTA
                     YYVNSEGTQYRIPENLSFLRVRGTALAPDGMLVRNGSVICVRDVSDLPSEAELRQRIT
                     TLAKNLSDTV"
     gene            complement(856..2532)
                     /locus_tag="VFQ79_17440"
     CDS             complement(856..2532)
                     /locus_tag="VFQ79_17440"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU0141511.1"
                     /translation="MHRFVLLVLLGAVGVAALAPQPELLDILAGELQRNFEVMRQKGD
                     PPPYYIAYTVTEEQSAAVSASQGTVQSSSADHRRILDVTVRAGSPELDNYHRLPGERP
                     RFAAPVSISLDNNPASIRQRLWLQTDQVYRAAAERLIKVKTSRQVKVTEEDLSADFSK
                     EEPVIAADPVIPLQFPMEEWRSRARSVSKVFTAHPQILESQVVVHANRQTKYFVNTEG
                     TRLRHGRPFSRITIAAGTKAADGMDLTLFESFDAAEPAGLPDEGELSKAVHRIAKKLE
                     SLSAAPLVEPFVGPAILSGRASGVFFHEIFGHRIEGHRQKDEGEGQTFTKKVGQEILP
                     DFLSVEFDPTKKNTNGVDLNGFYSFDDEGVKARPVPVVEKGVLKTFLMSRTPIEGFST
                     SNGHGRKAPGAEVVSRQSNLFVHSLRRFPQKQLRTMLIDEIKKQGKPYGLYFEEVTGG
                     FTMTGRRGLQAFTVIPVIVYRVYADGRPDEMVRGVDIVGTPLTSFSKVLATSNEVEVF
                     NGYCGAESGNVPVSAVSPALLISEIEIQKKDKSDQRPPLLPRPVVLGGGR"
     gene            complement(2630..3301)
                     /locus_tag="VFQ79_17445"
     CDS             complement(2630..3301)
                     /locus_tag="VFQ79_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0141512.1"
                     /translation="MTNTYIRRTVGICESQPITAEGLKSLLGQLPQFQFAGAASSLEA
                     ASELVAKKAPEILILDKTFGVQPLLHWMIQNNIAGRTSAVVVWGVSITESEALRYVQA
                     GAKGIIRKTADLNSITACLRAVSAGTNWMEDAVFRELPRRERYGRTDLTPREAQVLEL
                     VEQGLKNKEIARELGIRPGTVKIHLKHIFEKTGVKGRYGLALSGLKRAIAPAAAAPAA
                     GNSFH"
BASE COUNT          664 a          996 c          995 g          730 t
ORIGIN      
        1 ggacggtatc cgacaggttc tttgcgaggg tcgtaatccg ctggcgcaac tccgcttccg
       61 aaggaagatc gctcacgtcg cgaacacaaa tgacactgcc attgcgaacc agcattccat
      121 caggcgccag agcggtgccg cgcacgcgta gaaagctcag gttttccggg atgcggtact
      181 gcgtgccttc ggaattcacg taataggctg tggattgacc cgcctgaaac tcaacgtccg
      241 attcgataat ggccggataa ccggcgaata tcgccgaaag ctgtctcacg cgtgctttcc
      301 attcttcctg attgaccgta tagcggcgga cgggcaggat cttttgcgtg ggttgggctt
      361 tcgccagatc cgcgagaacc tccgactccg taacatgttg gagggcggcg cgtttgcgcg
      421 cgattgcctg cactgcccct ttataggcct gatcggttgc cagccagaaa aacgttcgca
      481 tggcgagcaa atcattttca agcggaaatc ccgccgagcc cacacggccg aacaggtccg
      541 tgaaaatgta gttggtgttg tcgaagtcgt agctgccgac gcggacctgg acacgcggca
      601 gcctgacgcg tgtgtgccgg ctgcctatca ggccgcccag actggcggaa gccgagaagc
      661 tgtcgagatc gtccagcgag tactcgatgt agtagggcga atcgagattt acgatgctga
      721 gctggcggga tcgctcgatc tcttcgcgca tggcgcgcag gatggggtcg gcggcttcct
      781 gggcgccggc aatgtgaagg gaggccgttg cacagacagc cagcaggaat ccgagcagcc
      841 tgcaggcgcc gcggttcatc ggcctcctcc gagtacgacc ggccgcggta gcaggggcgg
      901 ccgttggtcg gatttgtctt tcttctgaat ttcgatctcg gaaatgagca gagccggaga
      961 aactgccgaa acaggtacgt ttccggattc cgctccacaa tagccgttga aaacctcgac
     1021 ttcgttcgag gtcgccagca ctttggaaaa gctggtcagc ggtgtgccga cgatgtccac
     1081 tccccgcacc atctcgtcgg gtctgccgtc cgcatagact cgatagacaa tcaccggaat
     1141 cactgtgaaa gcttgcagcc cccgccgccc cgtcatggtg aacccgccgg tcacctcttc
     1201 gaaatagagg ccatagggtt ttccctgttt cttgatttca tcgatcagca tcgtgcggag
     1261 ttgcttttga ggaaatcgcc ggagcgagtg gacgaacagg ttggattgcc gggagaccac
     1321 ttccgcgccg ggcgcctttc ggccatgtcc gttggaggta ctaaagccct cgatgggggt
     1381 gcgcgacatc aggaaagttt tgagcactcc cttttcgacg accggcaccg gcctcgcttt
     1441 tacaccttcg tcgtcgaaag agtagaagcc gttcaaatcc acaccgttcg tgttcttctt
     1501 tgtcgggtcg aactccaccg acaaaaagtc cgggagaatc tcctgcccta ccttctttgt
     1561 gaaagtctgg ccctctccct catccttctg gcggtgccct tcgatgcgat gcccgaaaat
     1621 ttcgtgaaaa aacactcctg acgcgcggcc ggacaggatc gccgggccca cgaacggctc
     1681 taccaggggt gcggcggaga gcgattcgag cttcttcgcg atacggtgaa ccgccttcga
     1741 aagttcaccc tcgtccggca accctgcggg ttcggctgca tcgaagctct cgaagagagt
     1801 cagatccatg ccatccgccg ctttcgtccc ggctgcgatg gtgattcgcg agaaaggcct
     1861 gccgtgccga agccgtgtac cctccgtgtt gacgaaatat ttcgtctgac gattggcatg
     1921 caccacaacc tgcgattcga ggatctgagg atgagcggta aagaccttcg agacgctgcg
     1981 cgcgcgagat ctccattcct ccatcggaaa ctgtagcggt attaccgggt ccgcggcgat
     2041 cacaggctct tcttttgaaa agtcggcgga gagatcttcc tccgtgactt tcacctgcct
     2101 gctggtcttt actttgatca ggcgctcggc ggctgctctg tacacctggt cggtttgcag
     2161 ccacagacgt tgccggatgg aagccggatt gttgtcgaga gagatggaca ctggggcagc
     2221 gaaccgggga cgctcgcccg gcaaacggtg atagttgtcg agctcggggc tgccggcccg
     2281 gacggtgaca tccaggatcc gtctgtgatc ggccgaactg ctttgaactg tgccctggct
     2341 ggcggatacc gccgcgctct gttcttcggt gacggtgtag gcaatgtaat agggaggcgg
     2401 gtcgcccttt tgcctcatca cctcgaaatt gcgctgcaac tcgccggcga ggatgtcaag
     2461 cagttcaggc tggggagcca gcgctgcgac cccaacggct ccgagcagca ccagcaggac
     2521 aaatcggtgc acgctcaaca ggatatcaaa ggcacgtctc cggccgcgct tccggtatgg
     2581 tcgcagaaac ggagcagcgc gcgttaagca ggcggttttc tacgttttcc tagtgaaagg
     2641 aattaccggc cgcaggcgcg gcggcagccg gtgctattgc acgttttaac cctgataatg
     2701 ccagaccata ccggcccttt actccggtct tttcaaagat gtgtttgagg tggatcttga
     2761 ccgtaccggg ccggatcccc agttcccggg caatttcctt gtttttcagc ccctgttcga
     2821 ccaactcgag aacctgcgcc tcgcgcggcg tcaggtctgt gcgcccatag cgctcacggc
     2881 ggggcaattc gcggaaaact gcatcctcca tccagtttgt ccccgccgaa acagcacgca
     2941 gacacgcagt gatcgagttg aggtccgccg tcttccgtat aatgcctttg gcaccggcct
     3001 gcacataccg cagcgcttcg gactccgtga tggagacgcc ccataccaca acggcggagg
     3061 tgcgtccggc gatgttgttc tgtatcatcc agtgcagcag tggctgaact ccgaaggtct
     3121 tgtcgagaat cagaatctca ggagcttttt tagcgacaag ctcggatgct gcttccaaag
     3181 agctggcggc tccggcgaat tggaactgag gcaactggcc aagcagcgat ttcagacctt
     3241 cagccgtaat cggctggctc tcgcagatac ccaccgtcct tctgatgtat gtgttagtca
     3301 tggtgctgtc cgctcgcccg ggcacaactg cgcacgcaat tgccgctcac gggcattcat
     3361 cgacttcagc tactggatcg gcagt
//
LOCUS       DASTMK010000406         5663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_309224, whole genome shotgun sequence.
ACCESSION   DASTMK010000406 DASTMK010000000
VERSION     DASTMK010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309224"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFQ79_17450"
     CDS             complement(<1..767)
                     /locus_tag="VFQ79_17450"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141513.1"
                     /translation="MAVVLLALMLFAFAVPHAGAQTLYGTLVGNVTDPSNAAIGGAKV
                     VAVNTGTEFSREATTGERGAYLFSDLQPGTYRLNVSATGFAAFTQTDVQVSTNSVVRA
                     DIRMQLPTATESVTVAAAATALQSDRSDLRAEISGREYRDLPVPGARNYQALFKLVPG
                     FTPPRRQNSIVSTPQEGLVANVNGTTKSTNNTRIDGASNAHIWLPQHSAYMPPIEAIE
                     TVNVVTNSMDAEQGLAGGAASNVSMKSGTNEVHGVAF"
     gene            complement(904..2256)
                     /locus_tag="VFQ79_17455"
     CDS             complement(904..2256)
                     /locus_tag="VFQ79_17455"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEU0141514.1"
                     /translation="MKNIFSLLLVAAAIAVQGAYAQPAKHVVLISIDGFAAYHLYNQE
                     LRLPNIRELIDRGVWFASSETIFPSVTHPSHTTIVTGVEPRLHGVIGNSMVNRRTGER
                     FHPTNKPRKEIVRVPTIFDAAKARQLTTAAFFWPETKDDPAIDYNVPEVFTPDQKGDI
                     AAVNRKWIDELKNANVPIDLYFRWYGTERQGAGDAVLAEAAAYTIEKYKPNLLAIHIL
                     VTDKAQHQFGPHHYMASAALSMADHCVGILRRAVEKAGIERETAFIVTADHGFHSVYR
                     EINVRPVFEKAGLLDKVRLHGGGWTMAVELTDRFNGPSDMPALEKVFSRLRVEGIVRR
                     IASPEDMHELGLPRFEESEYASGQYFLIPDIDSYLVADAASSSTKPRPRATPAHSHGY
                     LPQHPRMYPILVLSGAGVKRNVNLPHARNLDIAPTIAHLLGIQLENMSGKVLRNALIQ
                     "
     gene            complement(2294..3028)
                     /locus_tag="VFQ79_17460"
     CDS             complement(2294..3028)
                     /locus_tag="VFQ79_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0141515.1"
                     /translation="MGAVPPGSDLAILEAEVIACRRCPRLVAYREHIAAVKRRAFRDW
                     IYWGKPVPSFGDPEARLLIIGLAPAAHGANRTGRMFTGDRSGDFLYRALHANGFASQP
                     ASERIGDGLELRDAYITASVRCAPPDNKPLPEEIRACRPFLERELDLLKRISVVVVLG
                     RLAFDVYLSILKDKGIIRRRSAFEFGHNRAHTIAPGQPLLLSSYHPSQQNTSTGKLTD
                     CMLRAVFEAAKMHIHSPELSRAEVPR"
     gene            3081..4394
                     /locus_tag="VFQ79_17465"
     CDS             3081..4394
                     /locus_tag="VFQ79_17465"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0141516.1"
                     /translation="MISRRGFIAVAPAVTTALSGASTPLAVLGGPKTRTEAFPGWPRI
                     GPLEEKELLGVLKSGHWYRGTGQNVNRFESKYAELTGAKHCLATANGTSALVTSLAGL
                     GIGPGDEVIIPPYTFIATVNAVLLHRALPVFVDTDLETFQIDAGKVEAAITDNTKAIM
                     PVHLGGAASDMDAIQAIASRRKLAVIEDACQAHLGEWRGRKVGSLGTTGCFSFQASKN
                     LNSGEGGAILTADEDLAERCFAFHNNGRGRKISGYDFSYRAGGANLRMTEFQAALLMA
                     QMTRLEEQSKTREENAAYLTKMLKEIPGILPAKMYEGCTRNAYHLYMFRYDKSAFADA
                     PRRAFLKALSGEGIPCSGGYAPLNKEPFLADTMNSKAYKTIYSARRLARWTERNQCPV
                     NDRLCEEAVWFTQTMLLGTKRDMEQIAEAIRKIHQQSGELRKVQA"
     gene            4497..4856
                     /locus_tag="VFQ79_17470"
     CDS             4497..4856
                     /locus_tag="VFQ79_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0141517.1"
                     /translation="MSAKIDVRQSMGVSILDVSGRITLGESSNLLRLTVKDLVAQQQR
                     NIVLNLSDVSYIDSGGLGEIVGSYATVANAGGRLKLLNPQKRVDDLLQVTKLLTVFEI
                     YTDEAAAVRSFQQAASA"
     gene            4869..>5663
                     /locus_tag="VFQ79_17475"
     CDS             4869..>5663
                     /locus_tag="VFQ79_17475"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0141518.1"
                     /translation="MRVALDATPLAGPVGGIRRYTVELSLALAREYSEDEFALVSDQA
                     FILPAGAPNLKAGRGPRNALESKWWTIGLARELSKLGTDVFHGTDFAVPYIPLRPSVM
                     TVHDLSPWIGPGKDDASMRVRRRTSILAGLGLATMIITPTASVCRQVLDCFRVSPHRV
                     AVTPLAASSRFRPNSACRGRFFLLPGTREARKNTAVLLQAWRELRKSVEVDLVLAGRS
                     RETEPDHEIPGLRILGEVSEEKLVSLYSEALAVIYPSVYEGFGLPVL"
BASE COUNT         1148 a         1618 c         1702 g         1195 t
ORIGIN      
        1 ttgaacgcga cgccgtgaac ctcgttggtg ccggatttca tactcacatt gctggcggcg
       61 cctccggcga ggccctgttc cgcatccatg ctgttggtca cgacattgac ggtctcgatg
      121 gcctcgatcg gaggcatgta ggccgaatgc tgcggcaacc agatatgcgc gttgcttgcg
      181 ccgtcgatgc gcgtattatt ggtgctcttc gtggttccgt tgacgttcgc gaccaacccc
      241 tcctggggtg tcgacacaat cgaattctgc cggcgcggcg gtgtaaatcc tggaaccagc
      301 ttgaacaatg cctggtagtt ccgggcaccg ggtacaggca ggtcgcgata ctcccgcccg
      361 ctgatctcgg cgcgcaaatc ggagcggtcc gattgcagcg cggtggcggc cgcggccacc
      421 gtgaccgact cggttgcggt cggcaattgc atgcgaatat ccgcccgaac caccgagttc
      481 gtggagacct gcacatccgt ctgcgtgaaa gcggcgaatc cggtcgcgct cacattaagg
      541 cggtacgtgc ccggttgcag atcggagaag agataggctc cacgttcgcc tgtcgtcgcc
      601 tcgcgactga actctgtacc ggtattcacc gcgacgactt ttgccccgcc gatggctgcg
      661 ttgctcggat ccgtcacatt tcccaccaga gttccgtaaa gggtttgcgc tcccgcatgc
      721 ggaaccgcga atgcaaacaa catcagcgca agcaggacca ctgccatcct gtcagatacc
      781 tgaagtatgg atgtgcctat aagtttgttc gttgagatca actttagctc cctctctggg
      841 gagctattat atccaggtcg gagcggtaca ttcgtaacaa attaaactgg gcagggcgcc
      901 gccttactgg ataagggcat tccgaaggac tttaccactc atattttcca actggattcc
      961 cagaaggtga gcgattgtgg gtgcaatgtc gaggttccgc gcgtggggca gattcacatt
     1021 ccgcttcact cccgcaccgg acaggaccag tattgggtac attcgcggat gctggggcaa
     1081 ataaccgtgg ctgtgcgccg gtgtcgcgcg cgggcgcggc ttggtggacg aactcgcagc
     1141 atcggcgacg aggtaggaat cgatatccgg aatgaggaaa tactgaccgc tggcatattc
     1201 actctcttca aatcgcggca gtccgagttc gtgcatgtcc tcggggctgg caatccgccg
     1261 tacgatacct tccacacgca aacggctgaa cactttttcg agggccggca tgtcggacgg
     1321 accattgaaa cggtctgtca actccacggc catggtccaa ccgccaccgt gcagccgaac
     1381 cttatcgaga agtccggctt tttcgaacac aggacgaacg ttgatttccc gatacacgct
     1441 gtgaaacccg tggtcggctg tcacgatgaa agctgtctcc cgctcgatgc ccgccttctc
     1501 gacggcacgc cgcagaatcc cgacgcaatg gtccgccatc gatagcgccg ccgaagccat
     1561 atagtggtgc ggcccgaact ggtgctgcgc cttgtcggtg acgagaatgt ggatcgccag
     1621 caggttcggc ttgtatttct cgattgtgta agcggcggct tcggccagca cggcatcgcc
     1681 cgctccctgc ctttccgttc cataccagcg gaagtagagg tcgatcggca cattcgcatt
     1741 cttcagctca tcgatccatt tcctgttgac agcggcgatg tccccttttt gatccggtgt
     1801 gaacacttcc ggtacgttgt agtcgattgc tgggtcgtct tttgtttcgg gccagaagaa
     1861 cgctgcggtg gtgagctgcc gggccttcgc tgcgtcgaaa atcgtaggca cgcgcacgat
     1921 ttcctttcgc ggtttgttcg tggggtggaa gcgctcaccg gtacgccggt tcaccatgga
     1981 attcccgatt acgccatgca gcctcggctc tactcctgta acgatcgtcg tgtgcgaagg
     2041 gtgcgttacg ctggggaaaa tcgtctcgct ggatgcgaac cagacgcctc tatcgatcag
     2101 ttcccgaata ttgggcagcc gcagttcctg attatagaga tggtatgctg cgaacccgtc
     2161 gatcgagatg agaaccacgt gcttcgccgg ctgcgcgtat gccccctgta ccgcgatcgc
     2221 agccgcaacc agcaacagag aaaagatgtt cttcataacc catcagcata gtccgaaaca
     2281 gggcgtgtac aaattagcgc ggcacttccg ctcgcgataa ctccggggag tgaatatgca
     2341 tcttcgccgc ttcgaacacc gcgcgcagca tacagtcggt cagctttccg gtagaggtat
     2401 tctgctggct cggatggtag gagctcagaa gcagaggttg ccccggagca atcgtgtgcg
     2461 cgcggttatg cccgaactcg aacgccgagc ggcgccggat aatccctttg tctttgagaa
     2521 tggagagata cacgtcgaaa gcgaggcggc caagaacgac cacaacgctt atccgtttca
     2581 atagatcgag ctcgcgctcg agaaacggac ggcacgcacg gatctcctcg ggaagaggtt
     2641 tgttgtccgg cggagcgcag cgtaccgagg cggttatgta tgcatcccgc aattccagtc
     2701 cgtctccaat gcgctcgctg gccggctgcg aagcgaaacc gttggcgtgt agagcgcgat
     2761 acagaaaatc gcccgagcga tcgccagtga acatgcgccc cgtcctgttg gctccgtggg
     2821 cggcgggcgc taatccgata atcagcagcc gcgcttccgg atcgccgaac gaggggaccg
     2881 gtttacccca atagatccag tcgcggaaag cgcggcgttt gacggcagcg atatgctcgc
     2941 ggtacgccac cagtctcgga cagcggcggc aggcgataac ttcggcttcg aggattgcca
     3001 aatccgagcc gggcggaaca gctcccattt cccaattgtc gcctggaaga gtaacatata
     3061 gcgggaggct cgcttaagtt atgatttcac gacgcgggtt tatcgcggtt gcgccggccg
     3121 tgactacggc gttgtcgggg gcgtccaccc cgcttgctgt cctcggaggt ccaaagacac
     3181 gaaccgaggc attcccaggc tggccacgca tcgggccact cgaagaaaag gaactccttg
     3241 gcgtactgaa gagtggtcac tggtatcgcg gaacgggaca gaacgtcaac cgttttgaaa
     3301 gcaagtacgc cgaactgacg ggcgcgaaac attgcctcgc aacggccaat ggcaccagcg
     3361 cgctcgtcac gtcgctggct gggctcggta tcggcccggg cgacgaagtg atcattccac
     3421 cttatacgtt catcgccacg gtgaatgccg tcctgttgca tcgcgcactt ccggtttttg
     3481 tcgatacgga tctggagaca tttcagattg atgcgggcaa ggtggaagcg gccatcacgg
     3541 ataatacaaa agcaattatg ccggttcacc ttggcggggc ggcatccgac atggatgcga
     3601 tccaggcaat cgccagccgc agaaagctgg ctgtcatcga agatgcgtgc caggcacacc
     3661 tgggagaatg gcgcggccgc aaggtagggt cgctcggcac caccggctgt ttcagcttcc
     3721 aggcatcgaa gaacctcaat tccggtgaag gcggcgctat ccttacggca gatgaagatc
     3781 tcgctgagcg ctgcttcgca tttcacaaca acgggcgcgg ccgcaagatt tccggctatg
     3841 acttttcgta ccgcgccggc ggcgccaacc tgcggatgac ggagtttcag gccgcgctgc
     3901 tgatggcgca gatgacccgg ctcgaagagc aatcgaagac tcgcgaggaa aacgccgcat
     3961 atcttacgaa gatgctcaag gagattccgg gtattctgcc ggcgaagatg tatgaaggct
     4021 gcacgcgcaa cgcgtatcac ctctacatgt tccgctacga caagagcgcg tttgccgatg
     4081 caccccggcg tgccttcttg aaggccctgt ctggagaggg cattccctgc tcgggcggat
     4141 atgcgccgct caataaggaa ccattccttg cggatacgat gaactcgaag gcgtacaaga
     4201 ccatctattc ggcccggcgc cttgcgagat ggacagagcg caaccagtgt ccggtgaacg
     4261 accgtctctg tgaagaagcg gtgtggttca ctcagacgat gctgctcggg acgaagcgcg
     4321 acatggaaca gattgcggaa gctatccgga aaattcatca acagtctgga gagctgcgaa
     4381 aggtccaggc ctgacggaac tgctgaaacg ccagtgcgtt ccggggcttg acttgcggac
     4441 gcgctcgcgc cattctttta ggcgcatcga agtgttccgg aagaccggag gttttcatga
     4501 gcgccaagat tgatgtgcgg caaagcatgg gcgtttctat actggacgtc agcggccgta
     4561 tcacgttggg agaaagttcc aacctgttgc gccttaccgt aaaagacctg gtggcgcagc
     4621 agcagcggaa tatcgtactg aacctttccg atgtgagcta cattgacagc gggggtctgg
     4681 gcgaaatcgt cggcagctac gcaacggtgg cgaacgctgg aggacggctg aaattgttga
     4741 acccgcagaa aagggtggat gacttgttgc aggtcaccaa gcttctgacc gtatttgaaa
     4801 tctacaccga tgaagcggct gcggtccgaa gcttccagca agctgcatcc gcgtgattcg
     4861 cactgcggat gagagttgcc cttgacgcga caccgttggc cggtcccgtt ggaggcatcc
     4921 gccggtatac cgttgaattg agccttgcac tggcccggga atattcggag gacgagtttg
     4981 cactcgtctc cgatcaggcg ttcatcctgc ccgcgggcgc gccgaacctt aaagcgggaa
     5041 gaggtccgcg aaacgctctc gaatcaaagt ggtggaccat cgggcttgcg cgggagctgt
     5101 cgaaactcgg gaccgatgtc tttcatggaa ccgactttgc tgtgccttac attccgctga
     5161 ggcccagcgt aatgaccgtt cacgatctct caccctggat aggccccgga aaagatgatg
     5221 cttccatgcg agtgcgcagg cgtacttcca tcctggcggg gctgggtctg gcgacgatga
     5281 ttattactcc cacggcctcc gtctgcaggc aggtgctgga ttgctttcgg gtttcgcctc
     5341 atcgggtggc cgtgacaccc cttgcggctt cgagccggtt ccgtccgaac agcgcgtgcc
     5401 gcgggcggtt ttttcttttg cccgggactc gcgaagctcg caaaaacaca gccgtgcttc
     5461 ttcaggcgtg gcgggagttg cgcaagtccg tggaggtgga tcttgtcctc gccgggcgta
     5521 gccgcgaaac ggagcccgat catgaaatcc cgggactccg tattctcggg gaggtctccg
     5581 aagagaaact cgtttcactt tatagcgagg ctctcgcggt gatttacccc tccgtttatg
     5641 agggattcgg cctgcctgtg ctg
//
LOCUS       DASTMK010000407        21739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_180601, whole genome shotgun sequence.
ACCESSION   DASTMK010000407 DASTMK010000000
VERSION     DASTMK010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21739
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180601"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFQ79_17480"
     CDS             complement(<1..650)
                     /locus_tag="VFQ79_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU0141519.1"
                     /translation="MAVVRIKVLFFGMLKDITGRSGEELDIDAVIIEDVFSHYARAFP
                     RIGELSSSILIARNQQFAGRGTAITEGDEIAFLPPVSGGSGEYTHEISEDEAGHFFAL
                     TRRPIDVRALAGRLLRGEDGAVVTFEGVVRNNTRGRPTKYLDYECYEPMAIKVMAGVG
                     REIAAASPIGRIAMVHRLGRMEIGETSVAIIVTAPHRKPAFEAALEGINRLKKLVP"
     gene            complement(686..2749)
                     /locus_tag="VFQ79_17485"
     CDS             complement(686..2749)
                     /locus_tag="VFQ79_17485"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEU0141520.1"
                     /translation="MPKRAIVFPILILLAASTTQADEGLWLFNRFPKDLVKERYNFEV
                     TDQFLDHLRLSSVRFNNGGSGSFVSPDGLLFTNHHVGAECIQQLSTPAQDYMANGFYA
                     ASQSDEKACPALEVNVLLDIQDVSGKVKAGVTDSTPDAEANRVRKANMSRLEKECTAE
                     TGTRCDVVTLYSGGQYHLYRYRKYTDVRLVFAPEYSIAAFGGDPDNFTYPRYCLDFAM
                     FRAYEKGRPATVSHFLKWSKEGVRDGELTFVPGHPGATGRLMTMAQLEFSRDLSYPLV
                     LARLHSQVRAIEAFGATSAENHRVAQEELQSAQNSYKAYSGFLRGLREPGLMARKRAE
                     EEKLRKAVQSDATSRARYGNAWNEIAAAYKTYRSIYKPYWLLESSATRGSRLMSLARN
                     IIRYAAEKQKPDGERLREFTDSALPSLEQEMYSTAPINPEMEKAVLTDYFRFLQQHLG
                     VQDATFKAILEGKTPKQAADHYVSTSRLDDVKYRKQLASDPAAIQKSTDGMIRLALIL
                     DEPARRYRKQYEDNVEAVVAANASKIAQAKFAVEGERSYPDATFTLRLSFGPVRGYED
                     AAGKRVPYATTVEGIYGRATGKPPFKLPERWLQSKAALRLNTPFNFVTTNDTHGGNSG
                     SPTVNTKGEIVGIIFDGNIEGLPNRFVFTDQQARSVHVASQGIVEALRTVYKAHRVLK
                     EIGAE"
     gene            2942..4297
                     /locus_tag="VFQ79_17490"
     CDS             2942..4297
                     /locus_tag="VFQ79_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141521.1"
                     /translation="MTQLGEAVARYNKLIETSPYRDLAWADELREQMQAKHLTVGGRP
                     ICPVLRPHFITRRQYKNLVKAAESLFGALDRIKHLALANPALLARMELLPAEKMLASV
                     DPGYQYLAVTSLLDTHIENGHLHFVECNSDTASGVVYGEMLSELFYDALPVKDFRKRH
                     TLTKLGGMKYFLQALLKAYKQFGGKKSPQIGILEFRQPFQTVDSGEYLLLREYFEREG
                     YPTQVIYPDQLEYRAGVLKQGDFQIDLIYRRVKAQEFLIRFDLTHPLVRAYREHAVCM
                     VNSFRSELGHKKAIFDLLTDDNITAGFPAAERRAIRDYIPWTRLVSATKTSYNDKAVD
                     LIDFILQNREKLVLKPNDDSGEQHTVHGSETDDAGWERALKAAMRNPYVVQERVAPAS
                     AMFPVLQWGALEMRELKVDVHPHSYLGKVQGCSSWVTADGPAGFSSISGLAPTFILES
                     R"
     gene            4376..7309
                     /locus_tag="VFQ79_17495"
     CDS             4376..7309
                     /locus_tag="VFQ79_17495"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU0141522.1"
                     /translation="MAHSQPDEGLLFKRLEEVLAPDALKVLTTLGLPGLSGQSDDRPA
                     LVVLAAGKGTRFGADPKCIQPVNGKPLAAHSIDAFHEIAPAPAVCVVGYRKEDVAAAL
                     GNRNVYVASANPAGGTGFAAFEALSVPELAERDFLLVLTMGDRVVPASIFRRILGVHH
                     EGAREAELTFLTAVHEVPRQAGKGRIRRNDSGRVLNIVEERDLPPDESPSGPVECNCP
                     LYVIRAKSLFRHLREVTNGNAQGQYYLTDIVQSIAEAGGEIRTLTITPEDEEYEILCA
                     DVTRPADLETLETVLTRRAAPPGGAIEKAVRAITADRHPGQIASIARQLQELVSTIAA
                     EDLSFRPDRPVAIGICGGRVRIAFMHPDMARFYGPAWQMPIGAATASGREQIIMLVQA
                     ADDGRVHLLPTSFKFRERIDSIPTADHMYPEQVSGWHEYEQFGTRMSESLLLSLGYFS
                     DEELEQRRRKELPVPPPALWVSNSMRRPFALVQNAIASLRTLSSGEHGSRVRNYLARD
                     TFRGLRLASTGSIPEGGFSSSSAVTVATQNAINALWDLGIGPDVLVQLACQAEYGTGV
                     RAGSLDQATEQKGRYNEGALMSSNPRENYRVIGTFPVPSDRFRMFFPYTVERDRTAWR
                     WSCGTYGEMAGSGPLTTGEMRKLTGKAAEIAAILLRLPLDRDFFQFIEQDLFEDGLLS
                     KENQRWICSVLQELPLLSSQDDLRTRVMANQSWYAEQMVTVNGMEPATAEQMAATTLE
                     SLFAGWRNPVLKRCDSSGNVVCEEGVPLRAMVAYLFGEVAKNFYLLHHPELWIEYVTL
                     SQRGDRCFDIDPTRLPSRADMERPLEWERDWSGPELLEEWLRRFQATPVDYNRGLDDA
                     ALTGEKLPEFHRLEGSNFFRGLALIDVAEAMLKRAFGENTVAVRVNAAGQGDYFQVHV
                     DRQKADPESVKSFIRNAFYRRFGLWSQPHFVEVHPGGGAVGVRLDRFDTLGTLIGKLQ
                     SWSAR"
     gene            7354..8376
                     /locus_tag="VFQ79_17500"
     CDS             7354..8376
                     /locus_tag="VFQ79_17500"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0141523.1"
                     /translation="MDRRRFLSAASALPLSLIGCGSSGNSDGETTLRNVSYDPTRELY
                     QEINQAFGREWQAAGNPAVVVEQSHGGSGKQARAVIDGLEADVVTLALAYDIDAIAER
                     SKFLPANWQQRLPNNSCPFTSTIVFLVRKGNPKNIRDWDDLAKPGTVVITPHPKTSGG
                     ARWNYLAAWGHASRKYGGDETKSQEFVSAVYRNVPVLDSGARGSTTTFAQRKLGDILI
                     GWENEAYLARKEFAADGFEIVVPPTSINAEPPVALVDHNTGKHGTRKVAEAYLQFLYT
                     PEGQEIAARHHYRPSLPEVAERHRADFQPVALFTVDEMFGGWKAAHEKHFRDGGLFDQ
                     IYQPGT"
     gene            8380..9204
                     /gene="cysT"
                     /locus_tag="VFQ79_17505"
     CDS             8380..9204
                     /gene="cysT"
                     /locus_tag="VFQ79_17505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEU0141524.1"
                     /translation="MARHRFIPGFGLTLGFTVFYLSVIVLVPLWFLVLRTTSLSWAQF
                     WAIVVDPRTLASLRLTLLASFAAAAVNAVMGFLVAWSLVRYQFPGKRAIDAIVDLPFA
                     LPTAVSGIALTAIYSSNGWLGSILEPLGIKVAFTPLGITVALIFIGLPFVVRALQPAL
                     EDLDPAVEEAAAILGAGRWQTFLRVILPAVTPALITGFTMAFARAVGEYGSVVFISGN
                     MPLKTEIVSLLIIAKLEQFDYAGATALGSLMLAISFVLLLAINSLQWWTARRYQGN"
     gene            9213..10067
                     /gene="cysW"
                     /locus_tag="VFQ79_17510"
     CDS             9213..10067
                     /gene="cysW"
                     /locus_tag="VFQ79_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEU0141525.1"
                     /translation="MIQTAKTTEPAWMRTILLGIALLFLLCFLVLPLAAVFTEALRRG
                     FGAYLASFRDPAALSAIRLTLLAAGIAVPANLVFGLAASWAIARFKFPGRNLLVTLID
                     LPFAVSPVISGLIYVLLFGMQGALGPWLAQHNIQIIFAVPGIVLATVFVTFPFVAREL
                     IPLMQEQGSEEEEAALLLGASGWQTFWRVTLPNIKWGLLYGVILCNARAMGEFGAVSV
                     VSGHIRGKTNTVPLHVEILYNEYNFVGAFAVASLLALLALLTLVIKSALEWKIRAETA
                     ALQAEEAA"
     gene            10064..11110
                     /locus_tag="VFQ79_17515"
     CDS             10064..11110
                     /locus_tag="VFQ79_17515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEU0141526.1"
                     /translation="MSIEVRNVSKTFGGYVALRNVSLCVGNGELVALLGPSGSGKTTL
                     LRIIAGLEVPDPADGSAVIVAGEEISVRNAQERRVGFVFQHYALFRYMTVFENIAFGL
                     RVRPRKERPAKAEIRERVNRLLELIQLDGLAHRYPAQLSGGQRQRVALARALAIEPKV
                     LLLDEPFGALDAKVRQELRRWLRRLHDEIHVTSVLVTHDQDEALEVADRIVVMNQARI
                     EQTGTPEEVFHNPASQFVMEFLGHVNLFHGRVESGKAVVSNDASAGAPIFVRPFDLEI
                     HREARGAAPMRARVKRIQSAGPSVKVELAGDNGQPILVEMSHDRLRELSLHRDDEVFV
                     NPREAKVFLDDYSI"
     gene            11162..11635
                     /locus_tag="VFQ79_17520"
     CDS             11162..11635
                     /locus_tag="VFQ79_17520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU0141527.1"
                     /translation="MKTLTKRTQYSLRALYALTRNYGSGPTLITQLSKEQAIPKKFLE
                     LLLLSLKGHGLVASKKGRGGGYYLAQPPDQITIGSVIRMIEGPLAPLPCASETRFRKC
                     DECLDVETCETRIVMREVRDAIAQILDRTTLAELCKRVDEAREKKQDAGALMYYI"
     gene            complement(11661..14795)
                     /locus_tag="VFQ79_17525"
     CDS             complement(11661..14795)
                     /locus_tag="VFQ79_17525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEU0141528.1"
                     /translation="MSEYAELQASSNFSFLRGASHPRELMAAAALSGLKAIALTDRDT
                     LSGIVLAHSFLKQNPTSGTRFIVGCRLDLANGASLLCYPTDRAAYGRLTRLLTLGKRR
                     AAKGTCDLSLADVAEYSEGQRFILPFPYALNEAAAGHIRTCAAMFPGRVHLALTHSCR
                     GDDRRWIQIVAEFARDIGIPAVVTNEVLYHSRERKPLQDVVTCIREGCTLRDAGFQLQ
                     ANAERDLKSPRQMHQLFSAYPDALEATLEIADACRFSLDELRYEYPEEIVEPGVSAYQ
                     DLERRAWAGAAWRYPEGIPDAVRKQIRHELQLIGEREYAPYFLTVHEIVKFAASKGIL
                     HQGRGSAANSVICYCLGITQVDPVKHGLLFERFISAARDEPPDIDVDFEHDRREEVIQ
                     HIYEKYGRDRAGLTATVVHYRTRRAVREVGKVLGLSEEVTSALAGSVWGWSNDGVLKE
                     SVREAGLDPSDRHLSLALRLTKMLVGFPRHLSQHVGGFVISRGRLDELVPVENATMKD
                     RTVVQWEKDDLDELGMMKVDILALGMLSAIRRAFELMERHYGRKLTLATIPHGDSAVY
                     EMLQRADSLGVFQVESRAQQTMLPRLKPKEFYDLVIEVAIVRPGPIQGDMVHPYLRRR
                     EGKEPVSYPSRELEAVLKKTLGVPLFQEQAMQIAIIGAGFTPTEADQLRRSLATFRHV
                     GTIATFRERFISGMLSNGYPQDFAERCFSQIEGFGTYGFPEAHAISFANLVYVSAWLK
                     CRYPDVFCAALLNSQPMGFYAPAQLVRDARSHGVEVRPIDVNRSEWDSTLEPVQGSTR
                     HAVRLGLRLVAGLAEKDALALVEARGEGYRSPDEIARRAGCGRTALDRLAQADAFGSM
                     QLGRRQALWKASALEGKMPPLFLHSEANLFEEPEVELPPLTPSQEVVKDYRATGLTLR
                     EHPLAFLRAGLRARRVITAEQLGRTSNGKVVTVAGLVLFRQQPMTAKNTIFLTLEDET
                     GAMNLIVWKHVHEKYRRAVYGGKLLGCQGVVQREGQVLHVVVNRVWDWSSDLLRLNSG
                     EKTPSLPVRSRDFR"
     gene            complement(14801..16267)
                     /locus_tag="VFQ79_17530"
     CDS             complement(14801..16267)
                     /locus_tag="VFQ79_17530"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEU0141529.1"
                     /translation="MIDRLERKDPQRRSSAFAVVKEEKNHLYIAAVNDPARDAGIQPG
                     ARLADARTLLPTLNIVLDDPEADEQLKQSLVEWCDRYSPLAAHDGPDGIALDITGCAH
                     LFGGEDALLEEMQTQLREMKFRVRGAIADTAGAAWALARFSKQAVVTREELTNALDPL
                     RVKALRIACETAAELDQVGLTTIGHIRRIPPDSLAARYGPGVLLRLDQVLGLAEEPIT
                     PYRAPAPYRAGRTFAEPIGTAAAIERTVLDLLRKLCTRLEKEHRGARQFDLDCYRVDG
                     SIVRQQVRTSKPGRSVTHLMRLFSERMSSFDVGFGIEIMILSAADVDTSAPVQMTLPH
                     CGDSIGKDTSLDEFLDRLGLRLGFDRVCRFQIRESLLPESSTEFVPVTNALPPSACWP
                     ENRVRPVRLIEPPAPIEIAEIIPGKSPVQIRIGRQLHRIVRAEGPERLTPEWWHDRPS
                     RWKTRDYYRIENERGARFWIFRETTRSGERWFLHGHLP"
     gene            complement(16308..17051)
                     /locus_tag="VFQ79_17535"
     CDS             complement(16308..17051)
                     /locus_tag="VFQ79_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141530.1"
                     /translation="MGSVFRTQAVSAPERAARLRETIEPLVAIRRRFSRTVPVSREID
                     AGLPYRGLPLGCVHEVRSSGLACGIAFAAMLAARISSPGGQIVYVASDRTFHPLGLLA
                     YGVQPERLIHVAVRRPLDLAWATLEALRCPQVDAVLAVVKTADLTLCRRFQLAAESHG
                     ATGFLLTDAASRAIASVASVITRWQVSPIPAPPGSTFEEPCWEIELSYCRGGRPGQWN
                     AAWRNGRLEPLASKPVRQAGFLHPNILAG"
     gene            complement(17150..17704)
                     /locus_tag="VFQ79_17540"
     CDS             complement(17150..17704)
                     /locus_tag="VFQ79_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0141531.1"
                     /translation="MGEILVQDITDTATVNRATFYDHYSDKFELFNSLVGADFQNLLE
                     ERNVCLDENCSSGLSAIVLAVGDYLQGLHRDHAACTRQTSSGPLIDAAVTLAIRRIVL
                     QGLKKQAGRFPVPREVTASLVSGAIYGAVKEWLSKTHWQVDEAALLSLVQPILPLLER
                     NAAPVCAAVAPRRKRSRNTIKPGR"
     gene            17912..18532
                     /locus_tag="VFQ79_17545"
     CDS             17912..18532
                     /locus_tag="VFQ79_17545"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0141532.1"
                     /translation="MTTLLQIDSSPLPGSVSREFTREFVRTWKTAHPQGEVIYRDLAA
                     DPPPPIDAGWIDAAYAAPAARRPPQTQTLALSDELIGELERADEYVIGVAMHNFSIPA
                     VLKLWIDQIVRSGRTFAYGKEGARGLLQGKKAAVLVASGGVYAPGTPAAAMNYIEPYL
                     KTILAFLGVTDVKFIAAGGTARMMSGAIDRPTFLKPVLEEVRAAAA"
     gene            complement(18671..19552)
                     /locus_tag="VFQ79_17550"
     CDS             complement(18671..19552)
                     /locus_tag="VFQ79_17550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0141533.1"
                     /translation="MNQSNWLAQRFEEHRGHLRGVAYRMLGSVSEADDAVQEAWLRLS
                     RVEAETIQNLSGWLTTVVARVCLDMLRSRKSRGEEPLEEGAATTVTDKTAGSDPEQEV
                     LLADSVGVALLVVLDRLSPAERLAFVLHDIFAVSFEEIAAIAGRTPEAARQLASRARR
                     RVQGVDSAASANLSGQREIVETFLSALRRGDFEGLLAVLDPDVVVRIDEAAARPGAPR
                     EIRGARKWAQGAIAYSQAARFVQPALVDGSVGLILAPGGKLSRVLRFIISNGKIVDAE
                     IIGDPARLREIDLAVLG"
     gene            19675..20181
                     /locus_tag="VFQ79_17555"
     CDS             19675..20181
                     /locus_tag="VFQ79_17555"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0141534.1"
                     /translation="MQARMNNPATVNPEAMKALHALWSAVEKSDVPQSTLGLIEMRAS
                     QINGCSVCVDMHARQLKQAGETGERLFTVAAWYDAPYFNEAERAALALTEAVTRLSDR
                     SDPVPDEIFNEAARHYDEQALAALILAIATINVWNRLNVSTRQIAGEWMKSAEAREWI
                     EKNHAAAR"
     gene            complement(20187..20687)
                     /locus_tag="VFQ79_17560"
     CDS             complement(20187..20687)
                     /locus_tag="VFQ79_17560"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEU0141535.1"
                     /translation="MTLLSFVLVLAAVLCSLVAGFLFAFAVVVMPGIAKLDDEGFIRT
                     FQAIDGVIQRSQPLFVFVWVGSVLALVSAALLGIRELDAANRLTLIVATLVYILGVQL
                     PTFTINIPMNNKLQKLDTGSISEPMKQRARSEFEPRWNRWNTIRTACSILTSVLLLLV
                     LLGRNP"
     gene            complement(20698..20907)
                     /locus_tag="VFQ79_17565"
     CDS             complement(20698..20907)
                     /locus_tag="VFQ79_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141536.1"
                     /translation="MEDPVSESKAVRAAVKGSYDQLHEVHRDIQAYVKTQSLKPTLTV
                     EEYTVVGMDKPDPKDWETNVYYLYE"
     gene            21109..>21739
                     /locus_tag="VFQ79_17570"
     CDS             21109..>21739
                     /locus_tag="VFQ79_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEU0141537.1"
                     /translation="MFGVLCVPGMYHHIKQLMYTVRIGVPDPRFGNMLLEQFGGANGE
                     LAAAMQYSIQGLNCDDPDRKDLLMDIGTEELSHLEVVGTLARMHLKPTKTDRRAAHAD
                     PLIAIAGGGGVNLFNSMGNAWTADYLKITGELDVDLRSNIAAEARAKIVYERLINFTD
                     DPGTIDALQFLMTREITHMKAFTAALESMGKPAFSIGRIPPTAGLVDQYF"
BASE COUNT         4333 a         6488 c         6553 g         4365 t
ORIGIN      
        1 attggaacca gttttttcaa ccggttgatg ccttcgagag ccgcttcgaa ggcgggcttg
       61 cgatggggcg ccgtcacgat gatggccacg cttgtttcac cgatctccat gcggcccagg
      121 cggtgaacca tggcgatccg gccgatcggg gaggctgccg cgatctcgcg gccgacgcca
      181 gccataacct taatcgccat cggttcatag cactcgtaat ccagatactt cgtgggacgg
      241 cccctggtgt tgttgcggac tacgccttca aacgttacca ccgcgccgtc ttcaccgcgc
      301 agcaagcggc cggctagtgc gcgtacgtcg atgggccggc gtgtgagcgc gaagaaatgg
      361 ccggcttcgt cctcggagat ctcgtgcgtg tattccccgc ttccgccact gaccggcggc
      421 aggaatgcaa tctcgtctcc ctcggttatc gccgtaccgc gtccggcgaa ctgctgatta
      481 cgcgcgataa gaatgctgga cgacaactcg ccgatgcgcg ggaacgcacg ggcgtaatgg
      541 ctgaagacat cctcgataat gaccgcgtcg atatccaact cttccccgga acggccggta
      601 atgtctttca acatgccgaa aaacagcact ttgattcgca caaccgccat actaacagac
      661 tgagttttcg tgctttgcgt tccctttatt ccgcgccgat ttccttcagg acacggtgcg
      721 ctttgtagac ggtgcgaagc gcttcgacga tgccctggct ggcgacgtga accgaccgcg
      781 cctgttgatc ggtgaacaca aaccggttgg gcaacccttc gatgtttcca tcgaatatta
      841 tgccgacgat ttcccccttg gtattcaccg tgggacttcc cgaattgccg ccatgcgtgt
      901 cgttggtggt gacgaaatta aacggagtat tcaaacgaag tgcggccttg gactgtagcc
      961 atcgttcagg cagtttgaaa ggcggcttac cggtcgcccg cccgtaaatc ccctccaccg
     1021 tggtggcata gggcacgcgc ttacccgcgg catcctcata gccgcgcacg ggcccgaagc
     1081 taagacgcag cgtaaaagtg gcatcggggt agctgcgctc gccctcgacg gcgaacttgg
     1141 cctgggcgat cttcgaagca ttcgccgcga caacggcctc cacgttgtcc tcgtactgct
     1201 tgcggtaccg gcgcgcgggt tcgtccagaa tcagggccag gcgaatcatt ccatcagtgg
     1261 acttctggat ggccgcgggg tccgaagcca attgtttccg gtacttcacg tcatcaagac
     1321 ggctggtgga gacatagtgg tccgcggcct gtttcggggt ctttccctcc aggatcgctt
     1381 tgaaggtggc atcctgcacc ccaagatgct gctggagaaa acgaaaataa tccgtcagga
     1441 cggccttttc catctccggg ttgatgggcg ccgtgctgta catttcctgt tcgagcgatg
     1501 ggagcgcgga atccgtgaac tcgcggagac gttcgccatc cggcttctgc ttctccgcgg
     1561 cataacggat gatgtttctc gcgagcgaca tcaaccggga acctcgcgtg gcactcgatt
     1621 cgagaagcca gtagggcttg tagatagatc gatacgtctt ataggcagcc gcgatttcat
     1681 tccatgcatt gccgtaccgg gcccggctgg ttgcgtcgga ctgaaccgct tttcgcaatt
     1741 tttcttcttc ggcgcgtttc cgcgccatca gtccgggctc tcggagaccg cgtagaaatc
     1801 cggagtaggc cttatagctg ttctgagcgc tttgcagttc ttcctgcgcg acgcggtggt
     1861 tttccgcact ggtggcgccg aacgcctcaa tagcgcgaac ctgcgaatgc agccgggcaa
     1921 gtacaagcgg gtaagaaagg tcgcggctaa actcgagttg ggccatggtc atcaggcggc
     1981 cggtggcgcc cggatggccg ggaacgaatg tcaactcgcc gtcccgaacg ccttccttgc
     2041 tccacttcag aaaatggctg acggtagcag ggcggccctt ctcgtacgcg cgaaacatcg
     2101 cgaagtcgag gcaatatcgc ggataggtga agttgtccgg gtcgcctcca aatgccgcga
     2161 tgctgtactc cggcgcgaaa accaaacgga cgtcggtgta ctttcgatac cgataaaggt
     2221 gatattgccc gccggaatag agggtcacga catcgcagcg agtcccggtt tccgccgtgc
     2281 actctttctc gaggcgcgac atattcgctt ttcgaacccg gttcgcctcg gcgtcggggg
     2341 ttgagtccgt tacgccggcc ttcactttac cggagacgtc ttgaatgtcg agcaggacgt
     2401 tcacttcgag cgccgggcag gctttctcgt ccgattgcga ggcggcatag aaaccgttcg
     2461 ccatataatc ttgcgccggc gtgctcaact gttgaatgca ctcggcgccg acgtgatggt
     2521 tcgtgaacag aagtccgtcc ggcgaaacga aggacccgga tccgccgttg ttgaatcgta
     2581 cggaggatag ccgcagatga tcgaggaact ggtccgtaac ctcgaagtta tatcgttctt
     2641 tcaccagatc tttcggaaaa cgattgaaca accacaggcc ttcatcggcc tgggtggtag
     2701 aggcggcgag aaggatcagt atcgggaaaa cgatggcgcg tttgggcata aatggcagga
     2761 agtttgagcg ttaacagttc gatgacgcat ctggggcggt acacgttaca gaagtttggc
     2821 aattcgtatg ctatacgatt atcaatataa ataacttatt tgttattagt aacttacagg
     2881 aaatcgcagt tcactttccc gacatgctgt gcgatactga gtgtagagat attttatagc
     2941 catgacgcag ttgggcgagg cggtagcccg atacaacaaa ctgattgaga cgagtcctta
     3001 cagggacttg gcatgggcag atgagctgcg cgaacagatg caggccaaac acctgaccgt
     3061 tggcggacgg ccgatttgcc cggtgttgag acctcatttt atcacgcggc ggcaatacaa
     3121 aaatctcgtg aaagcggccg aatcgttgtt tggtgcgctg gaccggatca agcacctggc
     3181 gcttgccaat cccgcgctac tggcgcgcat ggaactgctg ccggccgaaa agatgctggc
     3241 ttccgtcgac ccgggctatc agtatctggc ggtcacttcg ctgctcgaca cccacatcga
     3301 aaacgggcat ctgcactttg tggaatgcaa ctcggacacc gccagcggcg tggtttatgg
     3361 ggagatgctg tccgagttgt tctacgatgc tttgccggtg aaagattttc gcaagcggca
     3421 cacgctgacc aaactgggcg gcatgaagta tttcctccag gcgctgttga aggcgtacaa
     3481 gcaatttggc ggcaagaagt ccccgcagat cggcattctg gaattccgtc aaccctttca
     3541 aacggtggat tcgggcgagt atttgctgct tcgcgagtat ttcgagcgcg agggttaccc
     3601 gactcaggtg atctatcccg atcaactgga ataccgggcc ggcgtcttga agcagggaga
     3661 tttccagatc gatctgattt accgccgggt gaaggcgcag gaatttctaa tccggttcga
     3721 tctcacgcat ccgctggtgc gtgcttatcg cgagcatgcg gtgtgcatgg tgaacagctt
     3781 ccgttccgag ctcggccaca aaaaagcgat cttcgatctt ctcacggacg acaatatcac
     3841 ggccggtttt ccagccgccg aacgcagagc catccgggac tacattccct ggacgagact
     3901 ggtaagcgcg acgaagactt cttataacga caaggctgtg gatctgatcg acttcatcct
     3961 ccagaatcgc gagaaactcg tgctgaagcc gaacgatgat tcgggcgaac agcataccgt
     4021 gcatggttcc gagaccgacg acgccgggtg ggagcgggcg ctgaaagccg ccatgcgcaa
     4081 tccctacgtt gttcaggagc gagtcgcccc ggccagcgcg atgtttccgg tgttgcagtg
     4141 gggcgccctg gagatgcgcg aactgaaagt ggacgtgcat ccgcactcct acctcggaaa
     4201 agtgcagggc tgctcgagtt gggttacggc ggatggtccg gcgggtttct cctcgatctc
     4261 cggccttgcg cccaccttta ttctcgagag ccggtaaaac ctccataatt cgggcgcggg
     4321 taccatcact gcggccttct gtctgccgcg cctgataata atgaatacag gcgatatggc
     4381 tcactcacaa ccggacgaag gtctgctgtt caaacggctc gaagaggttt tagcgcccga
     4441 tgcattgaag gtattgacaa ctctggggtt acccggcctg tccggccaga gcgacgatcg
     4501 gcccgcgttg gttgtcctcg cggccggcaa aggcacccgg tttggagccg acccgaaatg
     4561 catccagccc gtgaatggca agccgctggc cgcgcattcc atcgatgcat ttcatgaaat
     4621 cgctccggcg cctgccgtat gcgtcgtagg ttaccgcaag gaggatgtgg cggcagcgct
     4681 gggcaaccgc aacgtctatg tggcatccgc aaaccctgcg ggaggaaccg gctttgcggc
     4741 gttcgaagct ctgagcgtgc cggagttggc ggaacgggat ttccttctcg ttttgacgat
     4801 gggcgaccgg gtcgttccag cgtcgatttt ccgcaggatt ctcggcgttc atcatgaggg
     4861 cgcgcgcgag gcggagctga cgtttctgac ggcagtgcat gaggtgcctc gccaggcggg
     4921 caagggccgc atccgccgca atgattccgg ccgcgtgttg aacatcgtcg aagagcgcga
     4981 cctgccgccc gacgaatcgc cgtccgggcc ggtcgaatgc aactgtccgc tgtatgtcat
     5041 tcgggccaaa tctctattcc gccatctgcg ggaagtgacg aacgggaacg cgcagggcca
     5101 gtattacctc acagatatcg ttcaatccat tgccgaagcc gggggagaga tccgtactct
     5161 gacgatcaca cccgaggacg aggagtatga aatcctatgc gccgacgtga cacggcccgc
     5221 ggacctggag actcttgaaa ccgttctcac ccgcagagcc gcccctcccg gtggcgccat
     5281 cgaaaaagca gttcgggcca ttaccgcgga tcgccacccc gggcagattg cgtccatcgc
     5341 gaggcaactt caggaactgg tgtccaccat cgccgccgaa gacttgtctt tccgtcccga
     5401 ccgtccggta gcgattggaa tctgtggcgg acgcgtccgg atcgcgttca tgcatcccga
     5461 catggcccgt ttttacggac cggcctggca gatgccgatc ggcgcggcca cggcgtccgg
     5521 acgcgagcag atcataatgc ttgtgcaggc ggccgacgac ggccgcgtac acctgctccc
     5581 gacgagcttc aaattccgcg agcgcatcga ttccatcccg accgccgatc acatgtaccc
     5641 ggagcaggtc tccggctggc atgagtatga gcagttcggc acgcgcatga gcgaaagcct
     5701 gctgctgtct ctcggctatt tcagcgacga ggagttggag cagcgccgcc gcaaggagct
     5761 gcccgtgccg ccgccggcgc tatgggtgtc caacagcatg cggcgtccgt ttgccctcgt
     5821 gcagaatgcg attgcctcgt tgcgcacgct ctcttccggc gaacacggca gccgcgttcg
     5881 gaactacctg gcgcgcgata cgttcagagg tctccggctg gcttcgaccg gtagcattcc
     5941 ggaaggcggt ttctcgagtt cttcggccgt caccgtggcg acgcagaacg ccatcaacgc
     6001 tctctgggac cttggcatcg gccccgacgt gctggtccag ctcgcttgcc aggcggaata
     6061 cggaacgggg gttcgcgcgg gctctctcga tcaggccact gagcagaaag ggcgctacaa
     6121 cgaaggcgcg ttgatgtcgt cgaacccgcg ggagaactac cgcgtcatcg gcacatttcc
     6181 cgtgccgtcc gaccggtttc gcatgttttt cccttacacg gtggaacgcg acaggacagc
     6241 ctggcgatgg tcgtgcggca catacggcga aatggccggt tccggtcctc tgacgaccgg
     6301 agagatgcga aagcttacgg gaaaggccgc cgaaatcgcc gccatcctgc tgcggctgcc
     6361 gctggatcgg gatttctttc agttcatcga acaggacctc ttcgaggatg gccttctgag
     6421 caaggagaac caacgctgga tctgctcggt acttcaagag cttcccctgc tctcctcgca
     6481 ggacgatcta aggacccgcg tgatggcgaa ccagtcctgg tatgcggaac agatggtgac
     6541 cgtaaacggc atggaacccg cgacggctga acagatggcc gcgaccacgc tggagtcgtt
     6601 gttcgcgggg tggcggaatc cggtgctgaa acggtgcgac tcctccggca atgttgtttg
     6661 cgaagagggc gttcctctcc gcgccatggt ggcctacctg ttcggtgagg tcgcaaaaaa
     6721 cttctacctg cttcatcatc ccgagctgtg gatcgaatat gtcacgctct cgcagcgagg
     6781 tgaccgctgc ttcgatatcg atccaacccg cctgccctcc cgcgcggata tggagcggcc
     6841 cttggaatgg gagcgggact ggagcggtcc ggaactcctg gaggagtggt tgcggcgatt
     6901 ccaggcgact ccagtcgatt acaatcgagg gctggatgac gcggcactaa ccggcgaaaa
     6961 gctgccggaa ttccaccggc ttgagggatc gaacttcttc cgcggccttg cgctgattga
     7021 tgtggcggaa gcgatgctaa aacgcgcatt tggcgaaaac acggtggcgg ttcgggtcaa
     7081 tgcggctgga cagggcgatt atttccaggt gcacgtagac cggcagaaag ccgatcccga
     7141 aagtgtcaag tccttcattc gaaacgcttt ctatcgccgt ttcgggcttt ggtcccagcc
     7201 ccacttcgtg gaggtccatc ccgggggtgg agccgtcggt gtacgcctcg accggttcga
     7261 tacgcttggg acgctgatcg gaaagctgca atcctggtca gcacgctaat gcctgattct
     7321 ctatcgaatt gatagaatgt gatccaggac ctaatggatc gccgacgttt tctctctgcc
     7381 gcttccgcac tccccctctc tctaatagga tgcgggtctt ctggtaacag tgatggggaa
     7441 acgacgctcc gcaatgtctc ctacgatccc actcgtgaac tgtaccagga aatcaaccag
     7501 gccttcggac gcgaatggca ggcggcggga aatccggcgg ttgtggtcga gcagtcgcac
     7561 ggcggctcag gcaagcaggc acgcgccgta atcgacggac tggaagccga tgttgtgacg
     7621 ctggcgctgg cgtatgacat cgatgctatt gccgagcggt cgaagttcct gcctgcgaac
     7681 tggcagcagc ggctgccaaa caacagttgt cctttcacgt caacgatcgt gtttctggta
     7741 cggaaaggca atccgaagaa cattcgggac tgggacgacc tggcgaagcc cggcactgta
     7801 gtcatcacgc cgcatccgaa gacctccggc ggcgcacgct ggaactatct ggcggcctgg
     7861 ggtcacgcaa gccggaagta cggaggcgat gaaacaaaat cccaggaatt tgtttcggca
     7921 gtttacagaa acgtccccgt acttgattcg ggcgcgcgcg gatcgacgac gacctttgcg
     7981 cagcgtaaac tcggcgacat actcatcgga tgggagaacg aagcttacct ggcgcggaag
     8041 gagtttgccg ctgacggatt cgagattgtg gtgcctccca cgagtatcaa cgcggagccg
     8101 cctgtcgcgc ttgtggatca caataccgga aaacatggaa cgcgaaaggt ggcggaggct
     8161 tacctgcaat ttctgtatac tcccgagggg caggagatcg cggcgaggca tcactaccgg
     8221 ccatccctgc cggaggtggc tgaacggcac cgtgccgatt ttcaaccggt cgcgctgttc
     8281 accgtggacg agatgttcgg cggctggaag gcggcgcacg aaaagcactt ccgcgacggc
     8341 ggattgttcg accagatcta tcagccggga acgtaatcgg tggcgagaca cagatttatt
     8401 cccggattcg gcctgaccct cgggttcact gttttttacc tgagtgttat tgtgctggtg
     8461 ccgttgtggt tcctggtgtt gcgtacgacg tcgctgagtt gggcccagtt ctgggcgatc
     8521 gtggttgacc cgagaacatt ggcttcgctg cggttgacgc tgctggcttc tttcgccgcg
     8581 gcggcggtga atgcggtcat gggtttcctg gtcgcgtggt cgctggtgcg gtaccagttt
     8641 ccggggaaac gcgcaatcga tgcaatcgtg gatctgccgt tcgcattgcc gaccgccgtc
     8701 tcgggcatcg cgctgaccgc gatctactcg agcaacggat ggctcggcag catactggag
     8761 ccgctgggga tcaaggtagc gttcacgccc ctcgggatca ccgtggcact catctttatc
     8821 gggctgccgt tcgtagtgcg cgcgctgcaa ccggcgctcg aagatctcga tccggcggtg
     8881 gaagaggccg cggccattct gggcgccgga cgctggcaga cgtttctccg cgtgattctg
     8941 ccggcggtta caccggcgct gatcaccgga ttcaccatgg cgttcgcgcg cgcggtgggc
     9001 gaatacgggt ccgtcgtgtt catctccggg aacatgccgc tgaagacgga gatcgtgtcg
     9061 ctgttgatca tcgccaagct cgaacagttc gactacgccg gcgccaccgc gctgggaagc
     9121 ctgatgctgg cgatctcctt cgtgctcctg ctggcgatca attcgctcca atggtggacc
     9181 gcgcggcgat atcaaggaaa ctgatttcgg aaatgataca aaccgcgaag actaccgagc
     9241 ctgcctggat gcgaacgatt ctgctcggca tcgcgctgtt gttcctgctg tgcttcctgg
     9301 tgctgccgct ggcggcggtg ttcacggagg cactgcggcg cggctttggg gcctacctgg
     9361 cgagctttcg cgatccggcg gccctgtcgg cgatccggtt gacgttgctg gcggcgggca
     9421 tcgcggttcc ggcgaatctc gtgttcggtc tggcggcatc ctgggcgatc gcccggttta
     9481 agtttccggg gcgcaatctg cttgtcacgc tgatcgacct tccgttcgcc gtgtcgccgg
     9541 tcatctcggg attgatctat gtgctgctgt tcgggatgca gggggcgctc ggtccctggc
     9601 tggcgcagca taacatccag atcatcttcg ccgtaccggg catcgtgctg gcgacggtgt
     9661 ttgtgacgtt cccgtttgtg gcgcgcgagc tgattccgct aatgcaggaa caaggcagcg
     9721 aggaagagga agccgcgcta ctgctgggcg caagcggctg gcagacgttc tggcgtgtga
     9781 cgctgcccaa tatcaagtgg ggattgctct acggcgtgat cctatgcaac gcccgcgcga
     9841 tgggcgagtt cggcgccgtc tcggtcgtga gcgggcacat tcgcggtaag acgaacacgg
     9901 tcccgctgca tgtcgaaatt ctgtataacg agtacaattt tgtcggtgcg ttcgcagtgg
     9961 cgagcttgct tgccctgctg gcgcttctga cactggtgat caagagtgca ctggaatgga
    10021 agattcgcgc ggagacggcg gcactacaag cggaggaagc agcgtgagca ttgaggtgcg
    10081 gaacgtcagc aagacgtttg gcggctatgt cgcgctgcgc aatgtcagcc tttgtgtcgg
    10141 caacggagag ttggtcgcac tgcttggccc ttcgggctca ggaaaaacga cgctgcttcg
    10201 catcatcgcc ggactggaag tgcctgaccc cgccgatggg agcgcggtaa tcgtcgccgg
    10261 ggaggaaatc tcggtacgca acgcccagga gcggcgcgtc ggctttgtgt ttcagcacta
    10321 tgctctgttc cggtacatga cggtattcga aaacatcgcg tttggattgc gagtgcggcc
    10381 tcgcaaggag cgccccgcca aggccgagat tcgcgaacgg gtgaaccggc tgctcgaatt
    10441 gattcaactg gacgggttgg cgcatcgtta tccggcgcag ctttccggtg gtcagaggca
    10501 acgtgtggcg ctggcgcggg cgctggcgat tgaaccaaag gtgctgctgc ttgacgagcc
    10561 gttcggtgcg ctggatgcaa aggtacgcca ggaactccgg cgctggctgc ggcgcttgca
    10621 cgacgagatc catgtgacca gtgtgttggt gacacacgat caggacgagg cgctggaggt
    10681 ggcagaccgg atcgtggtga tgaaccaggc gcggatcgag cagacaggta cgccggaaga
    10741 ggtgtttcac aaccccgcgt cgcaatttgt aatggagttc ctgggccatg tgaacctgtt
    10801 ccatgggcgt gtggagtcgg gcaaagccgt ggtatcgaac gacgcgtcag cgggagcacc
    10861 cattttcgtg cggccgttcg atttggagat tcaccgggag gcgaggggtg ccgctccgat
    10921 gcgggcgcgc gtgaagcgga tacagtcggc ggggccgagc gtgaaagtgg aactggccgg
    10981 agacaatggg cagccgattc tggtggagat gtctcacgac cggctgcgag aactctcgct
    11041 gcaccgggat gacgaggtgt ttgtgaatcc gcgggaggcg aaagtatttc tggatgacta
    11101 ttcgatctga cgtggttggt gccccgtgcc ggtgttgcat gcgaaactac aagcggagcc
    11161 cgtgaagact cttaccaaac gaacccaata cagcctgcgc gctctgtacg cccttacccg
    11221 caactatgga agcgggccga cgctcattac gcagctttcc aaggagcagg ctattccgaa
    11281 gaagtttctc gaactgctcc tgttgtcgct caagggtcac ggcctggtcg cgagcaaaaa
    11341 gggcaggggt ggtggatact atctcgcgca gccgccagat cagatcacga tcggttcggt
    11401 gatccgcatg attgaaggtc cgctggcgcc gctgccttgc gcgagcgaga cccgcttccg
    11461 caagtgtgat gagtgcctcg acgttgagac ctgcgagaca cggatcgtga tgcgcgaggt
    11521 gcgggatgcc attgcccaga ttcttgatcg aaccacgctc gccgaattgt gcaagcgggt
    11581 cgatgaggca cgcgagaaga aacaggatgc gggcgcgttg atgtactaca tctgagggag
    11641 caagaacccg cccaacaccg ctatcgaaag tcccggctgc gaacgggaag ggaaggcgtc
    11701 ttttcgccgc tattcagccg gaggagatcg cttgaccagt cccagacgcg gttgaccacg
    11761 acgtgcaaaa cctgcccttc cctctgcacc acgccctggc agccgagcag ttttcccccg
    11821 tagaccgcgc ggcgatattt ttcgtggacg tgcttccaga cgatcagatt catcgctccg
    11881 gtctcatctt cgagcgtcag aaaaatcgtg tttttggccg tcatcggctg ctgccggaac
    11941 agaaccaacc cggcaacggt aacgactttt ccgttggagg tccgtccgag ttgttccgcc
    12001 gtgatcacgc gccgcgcccg cagaccggct cgcaggaaag ccagagggtg ctcgcgcagc
    12061 gtcagccctg tcgcccggta gtctttcacc acttcctggc tgggcgtcag gggcggcagt
    12121 tccacttccg gctcttcgaa cagattcgcc tctgaatgca aaaataacgg cggcatcttt
    12181 ccctccagcg cgctcgcttt ccaaagcgcc tgccgccgtc cgagttgcat cgatccgaat
    12241 gcatcggcct gggccagacg gtccagcgcg gtgcgcccac acccggcccg ccgggcaatt
    12301 tcgtccggcg agcgatagcc ctcgccgcgt gcctcgacca gtgccagcgc atctttttcc
    12361 gcgaggcccg caaccaggcg cagtcccagc cgtaccgcgt gccgcgtgga gccctgcacc
    12421 ggttcgagcg tcgaatccca ttcgctccgg ttcacgtcga tggggcggac ctccacaccg
    12481 tggctgcgcg catcccgaac cagttgcgct ggcgcataaa aacccatcgg ctggctgttc
    12541 agcaaggcgg cgcagaacac gtccggatag cggcatttca accatgccga gacgtagacg
    12601 aggttggcga aggagatggc gtgcgcttcc ggaaagccgt acgtgccgaa gccttcaatc
    12661 tggctgaagc aacgctcggc gaagtcctgc ggatatccgt tggaaagcat cccgctgatg
    12721 aaacgctcgc gaaacgtcgc gatggtgcca acatgccgga acgtggccag ggaacgccgc
    12781 aactggtccg cttcggtggg ggtgaaaccg gctccgataa tagcgatctg catcgcctgc
    12841 tcctggaaca gcggaacacc caaagtcttt ttcaggaccg cttccagttc ccggctcggg
    12901 taggagaccg gctccttgcc ctctcttcgc cgcaggtaag gatgcaccat gtcgccctga
    12961 atcggccccg gccgcacgat ggctacttcg atgaccaggt cataaaattc tttcggcttg
    13021 agccgcggca gcatggtttg ttgtgcgcgg ctctccacct gaaaaacgcc cagggaatcc
    13081 gccctctgca acatctcata cacggcggaa tccccatgcg gaatggtggc taaagtaagt
    13141 ttcctgccat agtgccgctc catcagctcg aacgctcttc gaatggcgga gagcatgccg
    13201 agtgcaagaa tatccacctt catcatgccc aactcgtcca ggtcgtcttt ctcccactgc
    13261 acgacggtgc ggtctttcat cgtcgcgttc tcgacaggaa ccagctcgtc gagacgcccc
    13321 ctcgagatga caaatccgcc gacgtgctgg gataaatgcc gtgggaatcc gaccagcatt
    13381 ttcgtgagac gcagcgccag gctcaggtgc cggtccgaag ggtcgagccc ggcctcgcgc
    13441 acgctctctt tcaatacgcc gtcgttgctc caaccccaca ccgaacccgc gagcgcgctg
    13501 gtcacttctt cggaaagacc caggactttg cccacttcgc ggacggcgcg gcgcgtgcgg
    13561 taatgcacca cggtcgcggt caatccggcg cggtcgcgcc cgtacttctc ataaatatgc
    13621 tggatcacct cctcgcggcg gtcgtgctcg aagtcgacat cgatatccgg cggctcatcg
    13681 cgcgccgcgg agatgaaccg ttcgaacagc agcccgtgct tgaccgggtc cacctgggta
    13741 attccgaggc aataacagat caccgagttc gccgccgatc cgcggccctg gtgaagaatg
    13801 cctttgctcg cggcgaattt cacgatctcg tggaccgtca gaaagtacgg cgcatactcc
    13861 cgttccccga tcagttgcag ttcatgccgg atctgctttc tgacggcatc cggaattccc
    13921 tccgggtagc gccaggccgc tccggcccac gcacgccgct ccagatcctg ataggcggac
    13981 acccccggct ccacgatctc ctccggatac tcgtagcgca gctcatccag agaaaaccgg
    14041 caggcgtccg cgatttcgag tgtagcttcg agcgcatccg gataagcgga aaaaagctgg
    14101 tgcatctgcc ggggagattt caggtcccgt tcggcattcg cctgtaattg gaaccccgcg
    14161 tcccgcagcg tgcacccttc gcgaatacag gtgaccacgt cctgaagcgg cttgcgttcg
    14221 cgcgagtgat agagaacttc gttcgtaacc accgccggaa ttccgatatc ccgggcgaat
    14281 tcggctacga tttgaatcca acgccggtca tcgccccggc aggaatgcgt cagcgccaga
    14341 tggaccctgc cggggaacat cgcggcgcaa gtccggatat gcccggccgc cgcttcgttc
    14401 agcgcatacg ggaatggaag gataaaccgc tgcccttcgg aatattcggc cacatccgcc
    14461 aggctgagat cgcacgttcc tttcgcggca cgccgtttgc ccagcgtgag aagccgggtc
    14521 aggcgtccgt aagcggcccg gtccgtggga tagcaaagca aactcgcacc gttcgcgagg
    14581 tcaagcctgc aacccacaat gaagcgagtg cctgaggtgg ggttctgctt caggaaactg
    14641 tgcgcgagca cgatccccga aagcgtatcc cggtccgtca aagcaatcgc tttgagtccg
    14701 gacaacgccg cagccgccat cagttcccgt ggatgcgacg caccccgcaa gaaactgaag
    14761 ttcgaagaag cctgaagctc ggcgtattcg ctcatattcg ctacggaaga tgcccgtgca
    14821 aaaaccagcg ctctccggac cgggttgttt cgcggaagat ccagaatcgc gccccccgct
    14881 cgttttcgat ccggtaatag tcgcgcgttt tccagcgaga agggcgatca tgccaccatt
    14941 ccggagtcag ccgctccggt ccttcggcac gcacaatccg atgtaattga cggccgatgc
    15001 gaatctgcac cggtgacttg cccgggatga tttcggcgat ttcaatcggc gccggcggtt
    15061 cgatcaaacg caccgggcgc acgcgatttt ccggccagca ggcgctgggc ggcaaagcgt
    15121 tggtaacagg aacgaattcg gtggaggatt cgggcagcag gctttcacga atctggaacc
    15181 ggcacacgcg gtcgaagccc agccgcagcc ccaaacggtc gaggaactcg tccagcgagg
    15241 tatctttccc tatgctgtcg ccgcaatgcg ggagcgtcat ctgcaccggc gcggacgtgt
    15301 cgacatcggc ggcgctcagg atcatgattt caatgccgaa ccccacatcg aaggagctca
    15361 tcctctctga gaaaaggcgc atcaggtgcg tgacggaccg gcctggcttg gaggtgcgca
    15421 cctgctgccg gacgatgctt ccatccacgc ggtagcagtc gagatcgaac tggcgcgccc
    15481 cgcgatgctc tttttccaaa cgcgtgcaga gcttccggag cagatcgagt acggtgcgtt
    15541 caatggccgc cgccgttccg atgggttccg cgaaggtccg tccggcgcgg tagggcgcgg
    15601 gcgcacgata gggtgtgatc ggctcctccg caagtccaag cacctgatcc aatcgcaaca
    15661 acactcccgg gccatagcgg gccgccagcg aatcgggagg aatccgccgt atatgaccga
    15721 tcgtcgtcag cccgacctgg tcgagctcgg ccgcagtctc acacgcgatg cgcagcgcct
    15781 tcacccgcag cggatcgagc gcgttagtca gctcttccct tgtcactacc gcctgcttgc
    15841 tgaaacgggc gagcgcccat gctgcgcccg ccgtatccgc aatcgccccc cgaacgcgaa
    15901 acttcatttc gcgaagctgg gtttgcattt cttcgagcaa cgcatcttcg ccgccgaaca
    15961 aatgcgcgca gcccgtgatg tcgagcgcga tcccatcggg accatcgtga gcggccagcg
    16021 gcgagtagcg gtcgcaccac tccaccagac tctgtttcag ttgctcgtcc gcttccgggt
    16081 cgtccagcac gatgttcaag gtaggcagaa gcgttcgcgc atcggccagc cgcgcgccgg
    16141 gctggatccc ggcgtctctc gccggatcgt tgactgcggc aatgtagaga tggttttttt
    16201 cttccttcac caccgcaaag gcagaagatc ggcgctgagg atctttgcgc tccaggcgat
    16261 caatcatcag gcgaggaaag aataagcaca aaaaccgctg ccgcatctca tcccgccaag
    16321 atgttcggat gaaggaagcc tgcttgccgg actggtttgg atgcgagcgg ttccaaccgc
    16381 ccatttctcc acgcggcgtt ccactgccct ggacgtccac cccggcagta ggaaagttca
    16441 atttcccaac acggttcttc gaatgtcgat ccgggtggcg cggggatagg agaaacctgc
    16501 cagcgcgtga taacagacgc gaccgatgcg attgcccggg aggccgcatc ggtaagcagg
    16561 aaacccgttg caccatggct ttccgccgca agctgaaacc ggcggcaaag agtcagatcc
    16621 gcggtcttca cgacggccag gaccgcatcc acctgaggac agcgcaatgc ctccagcgtg
    16681 gcccaggcca gatccagcgg gcggcgcacc gcgacatgaa tcagccgctc cggctggacg
    16741 ccataagcca gcagccccaa cgggtgaaag gttcgatcag aagcgacgta aacaatttgc
    16801 ccaccggggg aagagatgcg cgccgccagc atcgcggcaa aagcaattcc gcaagcgagg
    16861 ccggagcttc tcacttcatg gacacagccg agcggcaagc cacggtaggg gagtccggca
    16921 tcgatctccc gggaaaccgg caccgttcgc gaaaatcgcc gccggatcgc tacaagcggc
    16981 tcaatcgtct cccgcaggcg ggcagcccgt tctggcgcag agaccgcctg ggttcgaaag
    17041 acactcccca tgacaggtcc agaatatcac aatattcgcc ttttattcgc ctagttcacg
    17101 ccactgtgtt gaggaaaggg gagcaagccg gcgtcgcggg atggccggat tacctgccag
    17161 gctttattgt gtttctcgat cgctttctgc gtggggcaac agcagcacaa acaggcgcgg
    17221 cgttccgctc gagaagagga aggatgggct gcaccagaga caatagggct gcttcgtcaa
    17281 cttgccagtg cgttttggac agccactctt tcacggctcc atagatcgcc ccactcacca
    17341 gggatgcggt cacttcacgc ggtaccggaa atctgccggc ctgttttttc agaccctgga
    17401 ggacaatcct tcgaatcgcg agtgtgaccg cggcgtcgat cagcggcccg gacgaggtct
    17461 ggcgagtaca ggcggcatgg tcgcggtgaa ggccctgaag gtaatcgcct accgcaagca
    17521 cgatggcgga tagtccggaa gagcagttct catcgaggca gacgttccgt tcttccagca
    17581 gattctgaaa gtcggcgccg accagggagt tgaagagctc gaacttgtcg ctgtaatggt
    17641 cgtagaaggt tgcgcggttc acggttgccg tgtccgtaat gtcctgaacg aggatctcac
    17701 ccaaaggttt ttgccggagt agttttctta agctgtcctg caggagccgc cgcgtgcgtt
    17761 ttatccgggg gtcgcgaaca ctttgatctg gcaacaaatt taaaccttct gctttcagat
    17821 gttatcagcg cttgaatctt tcgcacaaca tgtgtcgtct aagtaaacat caaacaatgc
    17881 ttaaacagca tatgttgtaa agaaggattc catgacgact ctcttgcaga ttgattccag
    17941 ccccttaccg ggttccgtga gccgcgaatt cactcgcgag tttgtccgga cctggaaaac
    18001 agcgcacccg cagggcgagg tcatttatcg cgacctggca gccgatccac ccccgcccat
    18061 cgatgccggc tggattgacg cggcatacgc tgcacccgcc gcccgccgcc cgccgcagac
    18121 gcaaacatta gccctgtcgg acgagttgat cggcgaactg gagcgcgctg acgaatacgt
    18181 gatcggggtg gcgatgcaca acttcagcat ccccgcggtg ctgaagcttt ggatcgacca
    18241 gattgttcga agcgggcgca cctttgccta tggtaaagag ggtgccagag gccttttgca
    18301 gggcaaaaaa gccgccgtcc tggtcgcaag cggcggggtg tacgcaccgg ggacaccggc
    18361 agccgccatg aattacattg agccgtacct gaaaaccatc ctcgccttcc tcggcgtgac
    18421 ggacgtgaag ttcatcgccg ccgggggcac tgccaggatg atgtcgggcg ccatagatcg
    18481 ccccactttt ctgaagcccg tattggaaga agtgcgcgcc gctgccgctt gaaacagaca
    18541 aacggcttgg acattaatac ccacccggga tatggtatat cgccgggaat gccgcacacc
    18601 atttcgggtg gccgctgtgg ccgagcgacg gtccgggctg ctgaatcctt tgtatattgc
    18661 ttgtgcgctg tcatcccaac acagccaggt cgatctcgcg aaggcgagcg gggtcgccaa
    18721 tgatctcagc gtctacgatc ttcccgttgg aaatgatgaa gcggagcact ctggatagct
    18781 tgccgcccgg tgccaggata aggccgaccg atccatcaac cagcgcaggc tgcacgaatc
    18841 gggccgcttg cgaatacgca attgcgccct gagcccattt ccgcgcgccg cgtatctctc
    18901 ttggcgcccc cggacgcgcg gcggcttcgt cgatccgcac gacgacatcg ggatccagca
    18961 cggcgagcag tccttcgaag tctccgcgcc gcagggctga gagaaacgtt tccacaattt
    19021 cacgctgccc ggaaaggttg gcagacgccg ctgaatcaac tccctgaacc cggcgccggg
    19081 cgcggctggc aagttgccgc gccgcttccg gtgtacgccc ggcgatggcg gcaatctcct
    19141 caaaagacac ggcgaagata tcgtgcaaga cgaaagcgag ccgctcggcg ggactcagcc
    19201 ggtcgagcac aacgagcagc gcgacaccaa cggaatcggc aagaagaact tcctgctccg
    19261 gatcgctccc ggccgttttg tccgtcacgg ttgtagccgc cccctcctcc agcggctcct
    19321 ccccgcgcga cttccgcgaa cgcagcatat cgaggcacac acgcgcaacc accgtcgtga
    19381 gccatccgct caggttctgg attgtctcgg cctcgacgcg gctcaaccgc agccaggcct
    19441 cctgcacggc atcatcagcc tcgctcaccg agccgagcat ccggtaggcc acgccgcgca
    19501 gatgcccgcg gtgctcttcg aatcgctgtg ccagccagtt gctctgattc atcgttcgtc
    19561 tccctcacgg agtgacggat gaatcctcgc aaacgtgaca gtcacagttt tccgtcgcga
    19621 tccgtcccgg tgttgacagt gcgaattcaa cacattccaa ggagatcaac aatcatgcaa
    19681 gcacgaatga acaatccggc aacggtcaac cccgaggcca tgaaggcgct gcacgcgctg
    19741 tggtcggccg tagagaaaag tgacgtgccg cagtccacgc tcggcctgat cgagatgcgt
    19801 gccagccaga tcaatggctg cagtgtctgc gttgacatgc atgcgcgcca gttgaagcag
    19861 gccggcgaaa ccggcgagcg tctctttacg gtggcggcct ggtatgacgc accatacttc
    19921 aacgaggccg aacgcgcggc gctcgctctt accgaagccg ttacccgcct cagcgatcgt
    19981 tccgaccctg tgccggacga aatcttcaat gaggctgccc ggcattacga cgagcaggcg
    20041 ctcgcggcgc ttatccttgc catcgcgacg attaacgtct ggaaccggct caacgtcagc
    20101 acacggcaga ttgccgggga gtggatgaaa tcggccgaag cccgggagtg gatcgagaag
    20161 aaccacgcgg cggcacggtg aaccggttac gggttccggc cgagcaggac aagcagcagc
    20221 aaaacggatg tgaggattga gcacgctgtc cggatggtgt tccatcgatt ccagcgcggc
    20281 tcaaactcgc tccgtgcacg ctgcttcatc ggctcgctta tcgagccggt gtccagtttc
    20341 tgaagcttgt tgttcatcgg tatgttgatg gtgaacgtcg gcaactgcac gccaagaata
    20401 taaacgagcg tggcaacgat cagtgtcaaa cgattggctg cgtccagctc ccggatgccc
    20461 aacaaagctg cggacaccag cgccagaacg gaacccaccc atacaaagac aaacaatggc
    20521 tggctgcgct ggatgacacc gtcaatcgcc tgaaacgtcc ggatgaaacc ctcgtcatcg
    20581 agcttcgcga tacccggcat caccacaacg gcaaacgcga acaaaaaccc ggccaccagg
    20641 gaacaaagaa cggccgccag caccagtaca aacgacaaga gcgtcatgtt cctcccttca
    20701 ctcgtacagg tagtacacat tcgtctccca atctttcgga tcgggcttgt ccatgcccac
    20761 cacggtgtac tcctcgacgg taagggttgg cttcaagctt tgcgtcttga cataagcctg
    20821 aatgtcgcgg tgaacttcgt gcagttggtc gtagctgcct tttacggccg cccgcactgc
    20881 tttggactcg gatactggat cctccataag aaattatgtc ttgcgtgctc accaggatgg
    20941 tcgtttggga atgccggaaa tcgacaaggg gtctcgtggc gagcttcgcc tttttgccgg
    21001 caaatctcct ctcagcattg cgtaaatttt gcaacgaccc taccgcttgc cgataatctt
    21061 ttcgcgcaag gcccttatga tcgcgtgctt tcggaagcat cggcttcggt gttcggcgtg
    21121 ctgtgtgttc ccggcatgta ccaccacata aagcaactga tgtacacggt gcggataggg
    21181 gtgccggatc ctcgattcgg gaatatgctg cttgaacagt tcggcggggc aaacggggaa
    21241 ctggccgctg cgatgcagta ttcgatccag ggcctgaact gtgacgatcc cgaccgcaag
    21301 gacctgctga tggatatcgg aaccgaggaa ctgagccatt tagaagtagt gggaaccctg
    21361 gcgcgcatgc atcttaaacc aacgaagacc gaccggcgcg cggctcatgc cgaccctctg
    21421 atcgccatcg ccggcggggg aggtgtcaat cttttcaatt ctatgggcaa cgcgtggaca
    21481 gccgactatc tgaagatcac cggcgagctc gacgtcgact tgcgcagcaa catcgccgca
    21541 gaggcccgcg cgaagatcgt ttatgagcgt cttatcaact tcactgacga ccctggaaca
    21601 atcgacgcgc tccaattcct gatgacccgg gaaatcaccc acatgaaggc atttacggcc
    21661 gcgctggaga gcatgggcaa accagccttt tccatcggtc gcattccgcc caccgcgggt
    21721 ctggtcgacc agtatttca
//
LOCUS       DASTMK010000408         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_147697, whole genome shotgun sequence.
ACCESSION   DASTMK010000408 DASTMK010000000
VERSION     DASTMK010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147697"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFQ79_17575"
     CDS             <1..721
                     /locus_tag="VFQ79_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141538.1"
                     /translation="RRFANGFQTQVSYTYSKAIAFTDEVDSSLSFNAPSALARNRSLT
                     NYDRTHNLQAAWAAELPFGRGKRWANQGGIGGALLGGWQLNGIFSSYSGTPFTVTSAG
                     TSLNAPANTQTADQVKPEVKILGGTGPGQAYFDPLAFAPVTDVRFGNSGLNILRGPGV
                     VNLDLGLFREFSATERVKIQFRAESFNVTNTPHFSNPGTNVSNLLLGADRSVSALRGY
                     AEILSAQEDERQFRLGLRISF"
     gene            847..2448
                     /locus_tag="VFQ79_17580"
     CDS             847..2448
                     /locus_tag="VFQ79_17580"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0141539.1"
                     /translation="MTRSSIRRTTIGLLFAVLLLLAYRGSGADRLEGRILDEKTGQPV
                     AATLLLSDGEGRPVEVDGEHSHVQYLGKRRCYVDGAFVLNARPSRLLIEVRRGLETVP
                     LQTEVDLTRHGSDPLTLRLRRWIDMREQGYVSGDTHVHFLSQAESHLQMRAEDLNVLN
                     LLVSDFTNDKEKFTGKLDPVSTPGHAVFVGQESRDWQNGHVLLLGINRIVEPFEPFGG
                     TFQGRNEPNLVMAKILREARRQGGVTTWAHFCNLPGAESPIDIALGLVDAVDLITYDD
                     PTQLPSHWGPWINSGMSQAEFTVMRSMDLYYQYLNSGFQLPIAAGTDKMGDYIPVGSN
                     RLYARPVSEPDYTGWLAGLKAGNGFVTNGPMLTFEVEGHTSGDVVPFTGSRKVKARAT
                     ARSILPFQSLEIVVNGETASIRNIASSRRPADGLYTLEVEGTFDLNRSSWIAARVAEN
                     PASKNRILPRGLTVFAHTNPVYFLQDGTKVRELSSVRYLQKYIQGTIHWLNTKARFRN
                     SSEREEALRLADQARRLYSDLEIQK"
     gene            2482..2988
                     /locus_tag="VFQ79_17585"
     CDS             2482..2988
                     /locus_tag="VFQ79_17585"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU0141540.1"
                     /translation="MAWQAELEPDRQFLRDLCEVRLAIEPTAAGFAAVRATTEELNEI
                     ALQLARRQILAQDGPLDQIIDGDLAFYAAVVVASHNPFLEYLSSAIRQPFRTALLYIS
                     RRPANLTLGLETHFKLLEALRSGDPLAARKAAEEAIGLAMVAVEEGARARTKPAVTRR
                     NRRGHHHA"
     gene            2985..>3919
                     /locus_tag="VFQ79_17590"
     CDS             2985..>3919
                     /locus_tag="VFQ79_17590"
                     /inference="COORDINATES: protein motif:HMM:NF023598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HEU0141541.1"
                     /translation="MTTESRALLAIETEAEFVFGQAPKPLHCGFDLTIGAGEVYPEVN
                     FTLPAIEITASSWPEIVSHYEDIATQIIRRAAALQVPGLVLEFELLPAMTETPEWGAE
                     ITGLLHRHLERAHGKTGLRSALRVTPTDIRDQLKPPVLRHGPAWDHLRRSFELCIAAG
                     ADIVSIESVGGKEVHDQALMYGEVPGILFALGVLAPRDMAWLWEKIVDFCKGTRAVPG
                     GDSACGFANTAMQLAHQKMLPEVLAAVVRAASAVRSLTAFEQGAVGPSKDCAYEGPIL
                     KAITGRPISMEGKSATCAHFSSVGNIAGAVCDLWS"
BASE COUNT          804 a         1156 c         1185 g          774 t
ORIGIN      
        1 gcggcgcttt gcgaacggat ttcagacgca ggtgagctat acgtactcca aagccatcgc
       61 gtttaccgac gaagtggaca gctcgctttc cttcaacgcg ccctcggctc tggcgagaaa
      121 ccgctcgctg accaactacg accgcacgca taacctgcaa gcggcctggg cggcggagct
      181 tcccttcgga cgcggcaaac gctgggcaaa ccaggggggc atcggtggcg cgcttctcgg
      241 aggctggcag ttgaacggga tcttcagcag ctacagcggc acacccttta cggtcacgtc
      301 ggcgggtacg tcgctgaatg ctccagcgaa cacacagacg gcagaccagg tcaagccaga
      361 ggtgaaaatc ctgggcggca ccggaccggg gcaggcatac ttcgatccgc tcgcattcgc
      421 tcccgtgacc gacgtccggt tcggcaacag cggcttgaac atcctgcgcg gaccgggagt
      481 agtgaacctc gatctggggc tttttcgcga attctccgcg accgaacggg tgaaaattca
      541 gttccgcgcc gagtccttta acgtcaccaa tacaccccat ttcagcaatc ccggaaccaa
      601 cgtttcgaac ctcctgctgg gggcggaccg ttccgtttcg gcgttgcgcg gatatgccga
      661 gatcctgagc gctcaggaag atgagcgcca gttccgcctg gggctgcgca tcagcttcta
      721 gagtgctgtc acaaccgctt gctcacgcgc gcggctcggt aacatctttg tttccgggcc
      781 gcgacggcca cggagcgttg cccgcgctgg agctatcatc atcaagagcg tgaaggcgca
      841 cccgttatga cacgaagctc cattcgcagg accacaattg gcttgctgtt tgcggttctt
      901 ctgttgcttg cctaccgggg ctcaggcgcg gaccggctgg aagggcgcat cctggacgag
      961 aagaccggcc aacctgtcgc agccacgctg ctgctctccg acggagaagg aagaccggtc
     1021 gaggtggacg gcgagcattc tcacgttcag tacctgggta aacgcagatg ttacgtggac
     1081 ggcgccttcg tattgaatgc ccgcccgagc cgtcttttga tcgaggttcg gcgcggttta
     1141 gaaacggtcc cacttcaaac ggaagtggac ctgacccggc acggaagcga tcccttgacg
     1201 ctccgcctgc ggcgctggat cgatatgcgc gagcagggct atgtgagcgg cgacacgcat
     1261 gttcatttcc tgtcgcaagc cgaatcgcac ctgcaaatgc gggcggaaga cctgaatgtt
     1321 ttgaatctcc tggtcagtga cttcacgaat gacaaagaga agttcacggg caaactcgat
     1381 ccggtatcga cgccaggtca tgccgtattc gtaggccagg aatcccggga ttggcagaac
     1441 gggcacgttc tcctgcttgg gatcaaccgg attgtagagc ccttcgaacc attcggaggt
     1501 acttttcaag gccgaaacga gcccaacctc gtgatggcaa agatcctcag agaagcgcgg
     1561 cggcagggtg gcgttaccac ctgggcccat ttctgcaatc tgccgggggc cgaatctccc
     1621 atcgacatcg ctctcggcct ggtggatgcc gtcgacctga ttacctatga cgatccaaca
     1681 caacttccca gccactgggg accctggata aactccggca tgtctcaagc cgagttcacc
     1741 gtcatgcgct ccatggatct ctattatcag tacctcaact cgggctttca acttccgatt
     1801 gccgcgggca cggacaagat gggcgattac attccggtgg gcagcaaccg gctttatgcg
     1861 cgtccggtga gcgagccgga ctatacagga tggctggccg gactgaaagc aggaaatggt
     1921 tttgtcacaa acggtccgat gctgactttc gaggtggaag gccacacgtc cggggacgtc
     1981 gtacctttca ccggaagccg caaagtgaag gcgcgggcga cggcccgttc cattcttcct
     2041 ttccagagtc tcgaaattgt agtgaatggc gagacggcgt ccatccggaa catcgcctct
     2101 tcgcggcgcc ccgcggatgg cttgtacacg ttggaagtgg aaggaacgtt cgatttgaat
     2161 cgaagcagtt ggatcgcggc gcgcgtagct gaaaatcccg ccagtaaaaa tcgcatcctg
     2221 cctcggggac tgaccgtttt tgcccacacc aatccggttt attttctgca agatggaacc
     2281 aaggtccggg aactgtcttc cgtgcgctac cttcagaaat acatccaggg gacgattcac
     2341 tggctgaata caaaagcccg gtttcgcaac tcgtctgaaa gggaagaagc gttgcgcctt
     2401 gcggaccagg ctcgccgtct ttattcggac ttggagatcc aaaagtaata atcatattgg
     2461 cggctgctgg atccggatat attggcctgg caagcggaac tggaaccgga tcgccagttc
     2521 ctgcgcgatc tgtgtgaagt gcgcctggcg atcgaaccga cggccgccgg gtttgcggcc
     2581 gtccgggcca caacggaaga gcttaacgag atcgcgctcc aactcgcccg gcggcagatt
     2641 ctcgctcagg acggcccatt ggatcaaatc atcgacggcg acctcgcatt ctacgcggcg
     2701 gtggttgtag ccagccataa cccttttctc gaatacctca gcagcgcgat tcgccagcca
     2761 ttccggaccg cgcttctcta catatcccgc cgtcccgcca acctcacgct tggccttgaa
     2821 acgcatttca agctgttgga ggcgttgcgg tcgggcgatc cactggccgc ccgcaaggct
     2881 gccgaagagg cgatcggact ggcgatggta gctgtcgaag agggcgcccg ggcgcgaaca
     2941 aaacctgccg taacgcggcg aaatcgaagg ggacatcatc acgcatgaca acagaatcgc
     3001 gggccctact ggcgattgaa acggaagcgg aattcgtctt cggccaggcg ccaaagcctc
     3061 tgcattgtgg gtttgacctg acaatcggtg ccggtgaggt gtacccggaa gtgaacttca
     3121 cgttgccggc aatcgagatc acggcatcga gctggccgga aatcgtgagc cattacgagg
     3181 acatcgcaac gcagatcatc cggcgggcgg cggcgcttca ggtcccgggg ttggtactcg
     3241 aattcgaact gctgccagcg atgacggaaa cgccggagtg gggcgcggag atcaccggac
     3301 tgctgcaccg tcatctagag cgcgcgcacg gcaagacggg cttgcgctcc gccttgcgcg
     3361 tcactcccac cgatattcga gaccagttga aaccacctgt cctgcgccat ggtccggcct
     3421 gggaccattt gaggcgcagc tttgagctat gcattgccgc gggcgctgat atcgtctcca
     3481 ttgaatcggt gggcggcaag gaggtccacg atcaagccct gatgtatggt gaggtgccgg
     3541 gcatcctttt tgcgctgggc gtgttggcgc cgcgggatat ggcgtggctc tgggaaaaga
     3601 tcgttgattt ctgcaagggc acgcgagccg tgcccggtgg agattcagcc tgcgggtttg
     3661 caaacaccgc gatgcaactc gcgcatcaaa aaatgctgcc cgaggttctc gccgcggtgg
     3721 tgcgcgctgc gtctgccgtt cgaagcctga cggctttcga acagggagcc gttgggccct
     3781 ccaaggattg cgcgtatgaa gggccgatcc tcaaagccat taccggacgc cccatctcca
     3841 tggaaggcaa atccgctacg tgcgcccact tcagttccgt cgggaacata gcgggagcgg
     3901 tgtgcgacct ctggagcaa
//
LOCUS       DASTMK010000409         8782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_436852, whole genome shotgun sequence.
ACCESSION   DASTMK010000409 DASTMK010000000
VERSION     DASTMK010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436852"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VFQ79_17595"
     CDS             complement(<1..1419)
                     /locus_tag="VFQ79_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141542.1"
                     /translation="MDSRSEHLPEEQLFDFREENPSRETAAHLASCPHCRQRLHDLQE
                     ACKAYEEYRTTIREPLLPPAPQTWPGLSVLIAGRKVKDRPWRFRWWPAAALAAASCLA
                     IAVVVFYGTGESSSRRAERILAQSAQVALPEGRMISLQMQGHTLVRPAVLKMDVPAAD
                     DPEMIHLRNLFVAANYSWEEPLSARSFLAWRDGLAKRRDEVSIVHGDGRRAYRVRTEN
                     PAGKLRAASLLLREHDMRPTEGSFDFEGETTLEVAETSMQPDRVPRNQRRPEIAQPEI
                     PAGPEDTIRVLAALRRLGADVEDPIAVVNDGRAVVVRGNGISPDRQQEIAAALHSIPR
                     VRLDFGSAGPVLSFAPPEAASERHSTNIPFSIRRKLEDRLGGPLAAQQTIDMVLDESS
                     SLMSHARALENVAAQFPETVEPGLSAQARKELEFLWNSHLSEMKEFLGRIREQLHSLT
                     AAAVVTPPVAVPQDLRESRVHQL"
     gene            complement(1412..1900)
                     /locus_tag="VFQ79_17600"
     CDS             complement(1412..1900)
                     /locus_tag="VFQ79_17600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0141543.1"
                     /translation="MNLHERVEKIYVSERENIYSYLLYLGVPAHRAQELAQDAFLKLY
                     LKMSKGEDIGDPRAWLYRVVHNLARRFHRREPVFDEIGPHMESLQTWTDPAGALIEKE
                     RRLNLARAIHDLSPQQRNCLHLRSQGLRYRDIAKTIGISSSAVGEFLRRAVVRLKEAV
                     GG"
     gene            2044..2682
                     /locus_tag="VFQ79_17605"
     CDS             2044..2682
                     /locus_tag="VFQ79_17605"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0141544.1"
                     /translation="MRHFLTFFLLACTTATGQIVSYGIKGGVPVTDAEQRPASFGNSF
                     FSYLNTGRLTIGPTIEFHLPANLSIEVNALYRGYEAKSAGTLQFGSELNPVFITMERD
                     VRAWDFPLLLKYRFPGDKMRPFLSAGVSLTRESTDTTVLYRCVGPLACYPDELTAFTG
                     GQFSHSRNREGYVGATGAEFRLRYIRIAPEVRYTRLQNPGANQVSVLVGISF"
     gene            2709..4085
                     /locus_tag="VFQ79_17610"
     CDS             2709..4085
                     /locus_tag="VFQ79_17610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0141545.1"
                     /translation="MKERQKVPLAAGVYLLIAIALCGVRPLWLDEILQLIDTRDGSPA
                     EIIGRLPANHPGGAPLGYLTQKAVLAVTGYSLRAARLPSAIFAAAAVFVVCLLAAELG
                     VRRSWLASILFAAFPLTLRYATEGRMYAEALLFATVATLLYLKLARRQTWGIAAAYAL
                     TLTAAAYTQPYSASVGAAHVLWSAGMRERRTALLGGTALAVTVGAFLPWYLWSREAWS
                     AAAAAYEFQFSLRTPLMIFREMTGAGYWGSGLLLILCVLGVMGGSNRRREHWFLLLMF
                     VVPVAAALIANALFGYFIATRQYLWVLPALAVFAAGAVERNGRLGTMLGVLLIAVCLV
                     QNARYFKGPQEDWETAAHAISRHVKCYGACVAVVPPAGLRLYQFFQPELGRSTCDPAE
                     VVLVVTPYTRAEDRAREVVRWTAEGYRNVNEVQVGGSLLLRFHRCPQVREDPQSGNQC
                     SWIRMSLK"
     gene            complement(4052..4657)
                     /locus_tag="VFQ79_17615"
     CDS             complement(4052..4657)
                     /locus_tag="VFQ79_17615"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0141546.1"
                     /translation="MIQRMLLLLCCAATLAVAADRFQSLFNGKDLSGWEVDTPGVWSV
                     NNGMIVGKASALKHNNFLRTRRKYRDFTLKINFRLVDGAGNSGIQFRSVPVTQSHEIS
                     GYQADIGETYWGCLYDESRRRKILAPASAAAVAGVNKDGWNTYLITAKGNHITLTLNG
                     ILSVDYEEKEPGMDIPGIIALQVHSGPRVEIYFKDILIQEH"
     gene            complement(4673..5749)
                     /locus_tag="VFQ79_17620"
     CDS             complement(4673..5749)
                     /locus_tag="VFQ79_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0141547.1"
                     /translation="MKAVILRQPRVLEVADVPKARMTDEHHVMVKVEACGICGSDLRY
                     WNGENPWALHTLGRHIPNPPNIILGHEFAGIVVEVNSSAYEHLLGERVGAQAFRVCGK
                     CNFCCSGRENLCRKTIHIGHAQGWGEMDLYPGAYAEYCLAWGDLVYPIPGNVPLEHAA
                     LADILCVAVHAVSRSKCTAGSVLCIGGGPAGLSIAQVAKAKGASKVFVSEPSPMAHNV
                     LQKFGNLVLIDPRQQRFSSVFGNSNGSCHMTAIYDSIGSAETIAEALPFLEESGTYVN
                     LAVHDTLVQMNAAVFGSEKTITSSSNALYSDVAKAYELICTQQIDLSPMITHRFSLDQ
                     CVEAFDLLLRSPKEAYKVILNPSN"
     gene            complement(5775..7019)
                     /locus_tag="VFQ79_17625"
     CDS             complement(5775..7019)
                     /locus_tag="VFQ79_17625"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141548.1"
                     /translation="MHQARSRPSRRGFLEHSALAAGYAATTFGYPANETLDVGCIGTG
                     GRCRELMRALQRIPGVRITAVADIWDANLNEGRRLADAKAFATKNYRDLLSRSDVDAV
                     VVGAPDHWHSQMAIDACAAGKDVYVEKPLTHSMAEGPKVIEAQNQHKRIVQVGTQQRS
                     MPHLIKARELVKSGQLGRIHKVHMSWNRNHQRRGPTTIDIDPTTVDWNSFLGNAPKQP
                     FDAYKLRNWRWFWDFGGGTLTDLMVHWLDTVNWFLDLDHPESAMSIGDTIHAKDLWQT
                     PDTMQTLLHYPQNELQLYFEGTFVNARCGAMTEFMGTEATLYIDRGRYEVIPERKRGL
                     RGEFVGNSLKPSELVLGDGPRGQDFYKTPEGEILHLGNWIECVRTRKRPNVPAEEGAK
                     AAAAAHLGNIAFRTGKVARWPG"
     gene            7198..7872
                     /locus_tag="VFQ79_17630"
     CDS             7198..7872
                     /locus_tag="VFQ79_17630"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0141549.1"
                     /translation="MLTSLGRSAAVPDQLVELFRNLILQGEWKPGSPIVETAIAKAVG
                     VAQPTVREALRHLEAEGLILRRQFRSCEVTQLSRDEVDQIFRLRMEWESLAAELAVEN
                     RANWKREHLLKAAEKLKQAARKHDSDSFYRYDLDFHKELWACTGNQFLSKALSQITVP
                     LFAFWTLRHLRESDVDLVKQAAAHERIALAIISKDKRQARKTTREAMQAFWKDGARVT
                     ADSRKH"
     gene            7889..>8782
                     /locus_tag="VFQ79_17635"
     CDS             7889..>8782
                     /locus_tag="VFQ79_17635"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141550.1"
                     /translation="MRPRIVLLAWLMASFLMLAQDFRATITGRITDPSGAVIAGSRVT
                     VTNTATRVGTEATTNEQGVFTVPYLIPGSYEVSAEAPGFKRAIQERIFLQTSDRLTLD
                     MKLEVGSATEVVEVREQSSLIDTSAAVAGTVISSRTVTQIPTLTRIPYLMASLSPGVV
                     ARDMNGTVPNAAGNATASGIRVNGGFGDTSNEYLIDGVPNNTTSRVAFIPSADAIQEF
                     KIVGNAYDAQYGRQAGSTINVTIRSGSNDLHGGAYHFYRDFNMSANSFQSNLSGRPKD
                     EWRYNLWGGQLGGPLYVPRLYD"
BASE COUNT         1729 a         2581 c         2542 g         1930 t
ORIGIN      
        1 cagttggtga acacgggatt cccggaggtc ctgcggaacc gctacgggag gcgtcaccac
       61 ggcggcggct gtcagggaat gtaattgttc tctgatgcgc ccgaggaatt ctttcatctc
      121 cgacaggtgg ctgttccata agaattccag ttccttccgc gcctgcgccg aaaggcccgg
      181 ctccacggtt tcgggaaact gcgccgcgac attttcaagc gcgcgcgcat gagacatcag
      241 cgaagagctt tcgtcgagca ccatatcgat tgtctgctgc gcagccagag gcccgccgag
      301 ccggtcctcc agtttgcggc ggatagagaa aggaatgttc gtggaatgac gttcgctcgc
      361 ggcttccggc ggagcaaagg acagcaccgg cccggctgat ccgaaatcaa gccgcacacg
      421 gggaatcgaa tgtagcgcgg cggcaatctc ctgctgccgg tccgggctga ttccgttgcc
      481 gcggacgacg accgcgcggc cgtcgttgac cactgcgata gggtcttcga catccgcccc
      541 gagccgccgc agcgccgcca gcacgcgtat ggtatcttcg ggcccggcgg gaatctccgg
      601 ttgcgcaatc tccggccgcc gctggttccg gggaacgcgg tccggttgca tggacgtctc
      661 cgccacctcc agcgttgtct ctccttcaaa atcgaacgag ccctcggtcg gccgcatgtc
      721 gtgttcgcgg agcagcagcg aggccgcccg cagtttaccc gcgggattct ctgtccggac
      781 acgataagcc ctcctgccat cgccgtgaac aatgctcacc tcgtcgcgcc gtttcgcaag
      841 cccgtcgcgc cacgcaagaa aggagcgggc gctcaacggc tcctcccaac tgtagttggc
      901 ggccacaaag agattccgca ggtgtatcat ctccgggtcg tccgcagcag gcacgtccat
      961 cttcaaaacg gcagggcgaa ccagcgtgtg gccttgcatc tgtagcgaga tcattcgtcc
     1021 ttccggtaat gcaacctgtg ccgattgcgc gaggatccgc tccgcgcggc gggagctgct
     1081 ctcgccggtt ccataaaaca ccaccacagc gatcgcaagg caggacgccg cggcgagtgc
     1141 ggcggcaggc caccagcgga atcgccaggg ccgatccttc accttccgcc cggcgatgag
     1201 gacgctcaga cccggccatg tctgtggggc gggaggcagg agaggttccc gaatcgttgt
     1261 ccggtattct tcatacgcct tacacgcctc ctggagatcg tgaagccgtt gccggcaatg
     1321 cgggcaggac gccagatgcg ccgctgtttc ccgcgacgga ttctcctcgc gaaaatcgaa
     1381 aagctgttcc tccgggagat gctctgaacg gctatccacc gacggcctcc ttcagccgga
     1441 ccacggcgcg ccgcagaaat tcaccaacgg cggagctgct gatcccgatg gtcttggcaa
     1501 tgtcccggta gcgcaggccc tggctccgca ggtggaggca attgcgctgt tgaggagaaa
     1561 ggtcgtggat cgcgcgcgcc aggttcaacc tgcgctcctt ttcaatgagc gcgcctgcgg
     1621 ggtcggtcca ggtttgcaaa ctctccatgt gtggaccgat ctcgtcgaac acgggctcac
     1681 gccggtggaa ccttcgggcg aggttatgaa ccacccggta cagccacgcg cggggatcgc
     1741 cgatatcttc tcccttcgac attttcaggt acagcttcaa aaacgcgtcc tgcgccagct
     1801 cctgcgcccg atgggcggga acacccaggt aaagcagata ggaatatatg ttctcgcgtt
     1861 cagagacata gatcttctcc actcgttcat ggagattcat gcctgcgact ggctcgcctg
     1921 catcagcacg ttccacgctt ctccataaga cactagagcc acacacaaca ggatttcact
     1981 acagcaaaga gaaatatttt ttgcctgtag gcagccggcc tctatccagg ctcttagcaa
     2041 ttgatgcgac acttccttac ttttttcctt cttgcctgta caacagcaac gggacaaatt
     2101 gtttcatatg gcataaaagg cggcgtgccg gttacagacg ccgaacaacg ccccgccagt
     2161 ttcgggaact cgtttttcag ttatctgaat acaggccgtc tgacgatcgg gcccaccatc
     2221 gagttccatc ttcctgccaa tctctctatt gaggtcaacg ctctctaccg gggatatgag
     2281 gcaaagtcgg cgggaactct tcaattcggc agcgaactca atccggtatt cattacgatg
     2341 gaacgcgacg tgagggcatg ggattttccg cttctgttga agtaccgttt cccgggtgac
     2401 aagatgcgtc cctttttgag cgcaggtgtt tcgttgactc gcgagtcgac cgataccacc
     2461 gtgctctaca ggtgcgttgg gccgcttgcc tgttatccgg acgaattaac tgcattcacc
     2521 ggcggccagt tcagccactc ccgcaaccgg gaaggttatg tcggcgccac cggcgctgag
     2581 ttccggctgc gttacatccg aatcgcgccg gaggttcgct acacgcggct acagaatccg
     2641 ggtgcgaacc aggtttccgt tctcgtcggc atctcgttct aaccggcttt caataacaac
     2701 tcgaaacgat gaaggaacgg cagaaagtgc cgcttgcggc aggcgtgtat ctgctcatcg
     2761 cgattgccct ttgtggggta aggccactct ggctcgatga gattttgcag ttgatcgaca
     2821 cgcgcgatgg gtccccggcg gaaataatcg ggcgcctgcc tgcgaaccat cccggcggtg
     2881 ctccgttagg gtatctgacg cagaaggcgg tgctggccgt gaccggatac tcgttgcggg
     2941 cggcgcgttt gccatcggcg atctttgcgg ctgcggcggt ttttgtggtc tgcctccttg
     3001 ctgcggagct cggggtccgc cggagctggc ttgcttcgat cctgtttgca gctttccccc
     3061 tgacccttcg ctacgcgacg gagggaagga tgtatgccga ggcgctgctg ttcgccactg
     3121 ttgcgactct actgtatctg aaactggcac ggaggcagac gtggggaatc gctgcggcgt
     3181 acgcccttac gttgacggcg gcagcgtata cgcagccgta ttccgcgtcc gttggagcgg
     3241 cgcacgtgct atggtcggcc ggcatgcggg aacggaggac ggcgctgctg ggcggtacgg
     3301 cactggcggt cacagttggc gcatttctgc cttggtatct atggtcccgc gaagcgtggt
     3361 cggcagccgc agccgcctac gagtttcaat tttcgctcag gacgcccctc atgatttttc
     3421 gagagatgac tggcgccggg tattggggct cgggcctgct cctgatcctt tgtgtgctgg
     3481 gagtgatggg cgggagcaac cgccggcggg agcactggtt tttgcttctg atgttcgttg
     3541 tgccggtcgc tgccgcgttg attgcgaatg cgctcttcgg ttatttcatc gcgacacgcc
     3601 agtatctctg ggttctcccg gcgctcgcgg tctttgccgc cggagcggtg gaacggaacg
     3661 gccgcctggg aacgatgctt ggcgtgctgc tgatagcggt ttgtctggtg cagaacgcac
     3721 gctatttcaa agggccgcag gaagactggg aaactgctgc ccacgccatt tcccggcacg
     3781 tgaaatgtta tggtgcatgt gttgccgtcg ttccccccgc aggcttgcgg ctgtaccagt
     3841 ttttccagcc ggaacttggc cgctcgacgt gcgatccggc tgaagtggtg ctcgtcgtga
     3901 cgccctacac acgcgcggaa gacagggcac gtgaagttgt gagatggact gccgaaggct
     3961 atcggaatgt aaacgaggtg caggttggag gttcgctgct tctccgcttc caccgttgcc
     4021 cgcaggtaag agaagaccct caatcgggga atcaatgttc ctggatcaga atgtctttga
     4081 agtaaatttc tacgcgaggg ccgctatgaa cctgcagggc gataatgcca gggatatcca
     4141 tcccgggctc cttttcctcg tagtcgacac tgagaatgcc gtttagggta agagtgatgt
     4201 gattgccctt ggccgtgatc aggtaggtat tccaaccgtc cttattcacc cctgccacag
     4261 cagccgcgga agccggtgcg agtatcttgc gccgccgcga ttcgtcgtaa aggcatcccc
     4321 agtacgtctc accaatatcc gcctggtatc ccgatatctc gtgggactgt gtgaccggca
     4381 cgcttctgaa ttggataccg ctgttgccgg caccatcgac gagccgaaaa ttgatcttca
     4441 aggtgaaatc tctatatttg cggcgtgttc gcaggaagtt attatgtttc agagcggacg
     4501 cttttccgac aatcatgccg ttgttcaccg accacacacc tggtgtatcc acttcccaac
     4561 cgctcaaatc ttttccattg aatagcgatt ggaaccgatc tgccgccact gccaatgtag
     4621 ccgcgcagca gagtagcaaa agcatgcgtt gaatcatcgc cgggtctcct gactaattcg
     4681 acgggttcaa aatcaccttg tatgcttcct taggcgaacg aagtaaaaga tcaaaagctt
     4741 caacgcactg gtccagagag aatcgatgcg taatcatcgg cgacagatcg atctgctgtg
     4801 tgcagatgag ttcgtaagcc tttgcgacgt cgctgtagag cgcattggag gaacttgtga
     4861 tcgtcttctc cgacccgaac acggctgcgt tcatttgcac tagcgtgtca tgcactgcca
     4921 aattgacgta ggttcccgac tcttcgagga agggaagcgc ttccgcgatg gtttcggcgc
     4981 taccgatcga atcgtaaatc gctgtcatat ggcaggaccc gttactgttt ccaaagaccg
     5041 agctgaagcg ctgctgtcga ggatcgatca gcacaagatt gccgaacttc tgcagcacat
     5101 tgtgggccat cggagagggc tcggaaacga acactttcga cgcgcccttc gctttcgcta
     5161 cctgtgcaat actcagaccg gccggcccac cgccgatgca gaggacgctc ccggctgtgc
     5221 atttggaacg gctgaccgca tgcacggcaa cgcacaaaat gtccgccagc gctgcgtgct
     5281 ccaacggcac gttaccggga atcgggtata ccaggtcgcc ccaggcaaga caatactctg
     5341 cgtacgcacc ggggtagaga tccatttctc cccagccttg cgcgtgaccg atatggattg
     5401 tcttccggca cagattttcg cggccggaac agcaaaagtt gcacttccca caaacgcgga
     5461 acgcctgtgc tccaacgcgt tcgccaagca aatgctcgta ggccgatgag ttcacttcga
     5521 ccacgattcc cgcaaactcg tgaccgagta tgatattcgg cgggtttgga atatgccgcc
     5581 cgagcgtgtg cagcgcccat ggattttcgc cgttccagta gcggaggtcg ctgccgcata
     5641 tcccgcaagc ttcgaccttg accatgacgt ggtgctcgtc tgtcatgcgg gccttgggca
     5701 cgtccgcgac ctcgagcacg cgtggctgcc ggagtataac tgctttcatc gtgggacacc
     5761 taacgacagt cgcatcagcc tggccacctg gccacctttc cggttcgaaa ggcgatgttt
     5821 cccaggtgtg ccgccgctgc cgctttcgca ccctcttccg ctggtacgtt cggacgcttt
     5881 cgtgtccgaa cacactcgat ccagttaccg agatgcaaga tctccccctc cggagtcttg
     5941 tagaagtcct gtccacgagg accgtcgccg agcacgagtt cactgggttt tagcgaattg
     6001 ccgacaaact ccccacgaag gccccgtttc cgttccggta tcacctcata gcgtccccgg
     6061 tctatgtaga gcgttgcctc ggttcccata aattcggtca tcgctccaca acgggcattc
     6121 acgaatgtgc cctcaaagta caactgaagc tcgttttgcg gatagtgaag aagcgtctgc
     6181 atcgtatccg gcgtttgcca caggtccttt gcatggatgg tatcgccgat tgacattgcg
     6241 ctttccggat ggtccagatc gaggaaccag ttcaccgtat ccagccagtg caccatcaga
     6301 tccgtcagag ttcctcctcc gaaatcccag aaccaccgcc agttccgcag cttgtaagcg
     6361 tcaaatggct gcttcggcgc gttgccgaga aagctgttcc aatcaaccgt tgtagggtcg
     6421 atgtcaatcg tcgttggtcc gcgtctctgg tggttgcggt tccaactcat atgcacctta
     6481 tgaatcctgc caagctgccc ggacttcacc agctcgcgag cctttatgag atgaggcatg
     6541 ctgcgctgct gcgtgccgac ctgcacaatg cgcttgtgtt gattctgcgc ttctatgact
     6601 ttcggccctt cagccatgct gtgcgtaagc ggcttctcga catacacgtc tttaccggcg
     6661 gcgcaggcgt caatggccat ctgtgagtgc cagtggtcgg gtgccccgac gaccaccgca
     6721 tcgacgtcgg aacggctgag caggtcccga tagttcttcg tggcgaatgc cttcgcatcc
     6781 gccagcctgc ggccttcgtt cagattggcg tcccagatat ccgcgacggc ggtaattcgc
     6841 acaccgggta tgcgctgcaa tgcgcgcatg agctcgcggc agcgtccacc ggttccgatg
     6901 caacccacat cgagtgtttc gttagccgga tacccgaacg tggtggcggc atacccggcg
     6961 gccagcgctg aatgttcgag aaaaccgcgc cggcttggcc tgctccgcgc ttgatgcatg
     7021 acaccttcct cattaacgat caacgatgaa agtttatatc agaattaacg cgagctgtca
     7081 attttttcgt tgacaggacg tttggaggac tataagctat catcgatgat cgttttaaga
     7141 aataatcttc cagaagttac aaaacgcagc ggcccaatct cgcagatccc gccgcagatg
     7201 cttacatcgc tcgggcgctc ggccgccgtt cccgaccaac tcgtggaact gtttcgaaac
     7261 ctgattctgc agggtgagtg gaagcccggc tcgcccatcg tcgaaacggc tattgccaag
     7321 gcggttggag tggcgcagcc caccgttcgt gaggcgctga ggcacctcga ggccgaaggt
     7381 ttgatcctgc gcaggcaatt tcgcagttgc gaagtgacgc agttgtcgcg cgacgaagtc
     7441 gaccagatct tccggcttcg tatggagtgg gaatcgcttg ccgccgagtt ggcggtcgaa
     7501 aaccgggcaa actggaaacg cgaacatctt ttgaaggcgg ccgaaaagct caagcaggcc
     7561 gcgcgaaaac atgacagcga ttccttctac cggtacgatc tcgattttca caaggagctc
     7621 tgggcctgca cgggcaatca gttcctgtcg aaggcgctgt cgcagattac agtaccgttg
     7681 tttgcattct ggacgctgcg gcacctgcgg gagtccgatg tcgacctcgt gaagcaggct
     7741 gccgctcatg agcggattgc cctggccatt atttcgaaag acaagcggca ggcacggaag
     7801 acaacgcggg aggcgatgca ggcgttctgg aaagatggcg ctcgcgttac cgctgactca
     7861 aggaagcact aagagagagg ggaatcgtat gcggcctcgg atcgttttac tcgcatggct
     7921 gatggcttcg ttcctgatgc tggcacagga ttttcgtgcc accatcaccg gtcgtatcac
     7981 cgatccgagc ggcgcggtaa tcgccggatc cagagtcacc gtcaccaaca cagctacgcg
     8041 agttgggacg gaggcgacga caaacgaaca gggagtgttt actgtaccct atctgattcc
     8101 ggggtcttat gaagtgagcg cggaagctcc ggggttcaag cgggcgattc aggaacgaat
     8161 tttcctgcaa acatccgatc gtctgacgct cgacatgaag ctggaggttg gttcggctac
     8221 ggaagtggtc gaagttcgcg agcagtcgtc gctcatagat acttccgccg ccgttgccgg
     8281 aactgtgatc tcgagccgga cggttaccca gatcccgacg ctaacccgta ttccttatct
     8341 gatggcgagc ctgtcgcccg gcgtggttgc tcgcgatatg aacggaactg ttccgaacgc
     8401 agcgggaaat gctaccgctt ccggcattcg tgtcaatggt gggttcggcg atacgtcgaa
     8461 tgagtacctg attgacggcg tgccaaacaa cacaaccagc cgggtcgcct tcattccctc
     8521 ggccgacgct atccaggagt tcaagatcgt cggaaacgct tacgatgctc agtacggacg
     8581 gcaagccggc agcacgatca atgtcaccat tcgcagcggt tcgaacgatc tccacggcgg
     8641 tgcgtatcac ttttaccgcg acttcaatat gagcgcgaat agcttccagt cgaatctcag
     8701 tggccgtccg aaagatgagt ggagatacaa cctgtggggc ggtcaactgg gcgggccgct
     8761 ttatgttccg cgcctttacg at
//
LOCUS       DASTMK010000410         7176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_628976, whole genome shotgun sequence.
ACCESSION   DASTMK010000410 DASTMK010000000
VERSION     DASTMK010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628976"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFQ79_17640"
     CDS             <1..201
                     /locus_tag="VFQ79_17640"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEU0141551.1"
                     /translation="DAVGIGTDFDGVSCVPVGFEDVSRFPALEKALLGKGYSAAQIGK
                     IFGGNILRVMRSAEQARGSPSN"
     gene            246..857
                     /locus_tag="VFQ79_17645"
     CDS             246..857
                     /locus_tag="VFQ79_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141552.1"
                     /translation="MTRREFVSAAAAVSSSPALLVIPVHQVIDARAKHTPEQLHRFSS
                     GIWTEAVRDFRRCGIQFQGTQGPGEIRRSPGGRPVFNGLRDGMINLILTGHLPMAWDK
                     GRALPGLSTRHEGRPICIIALRYAHGHQIPFVSVNTCVHELLHVLLGDIFESRPKGLP
                     GFGRELRIDWYATRLWLFRDGAAIREAARAYLDRLRTELAART"
     gene            complement(863..1807)
                     /locus_tag="VFQ79_17650"
     CDS             complement(863..1807)
                     /locus_tag="VFQ79_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0141553.1"
                     /translation="MSKFVVTGGAGFIGSSVVRALLKDGAPRVTVIDNLLSGKRENLD
                     EVKDRVDFHLADIRHYDEIRPLLEGAGAVFHLAAIPSVPRSIKDPVPSHEVNVDGTFQ
                     LLRAAADAGARRVVYAASSSAYGDTEVLPKVETMVPNPQSPYAAQKLFGEYYCSVFSK
                     CFNLETVALRYFNVYGPRQDPSSPYSGVLSIFMRCVAERRSPVIFGDGEQSRDFTYVE
                     DVAALTVRAAVKGVSGRVYNAGNGNRYTLNEIWKLVQGLEGVSLPASYGPSREGDVRH
                     SQADTAAAARDLGHSPRFSIEEGLKRTLHWYKEQSVAA"
     gene            complement(1807..2643)
                     /locus_tag="VFQ79_17655"
     CDS             complement(1807..2643)
                     /locus_tag="VFQ79_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0141554.1"
                     /translation="MSYQFKQSICNEVFKDWSFSDTCKAIRKAGYQGIEIAPFTLAED
                     PAKITAAARKEYRDVMASEGLTFVGLHWLMVSPKGLHVTTADKKLREKSWQHIRVLID
                     LCADLGPDGVMVFGSPQQRSTTGRLTAPEATRNFIEGLTEVAGQAAGRGVTILVEALP
                     RAQCDVVQSVSEAVEIVKGINSPAIRTMFDTHNAVDEAEEHATIVDRYFELIRHVHIN
                     EMDGRHPGTGSYDFKPVLQVLKNRNYQGWVSLEVFDFSPGAEKIANESLRYIESEISR
                     LA"
     gene            complement(2689..4098)
                     /locus_tag="VFQ79_17660"
     CDS             complement(2689..4098)
                     /locus_tag="VFQ79_17660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0141555.1"
                     /translation="MRLSVLVPVYNEEVVVERCLKQVLSTPLPENLDREVILVDDCST
                     DGTFGILERLASEHPEIRLFRHPVNKGKGAAIRTAVQQATGDFSIIQDADLEYDPSEY
                     SRLLRPLLDGRADVVFGSRYLAGEQTRVLPFWHSVINQGLTLVSNMFCNQNISDMETG
                     YKVFRTDLLKSIPIRSDRFGFEPEVTVKCAKRHFRIYEVPISYHGRTYEEGKKIRWRD
                     GLNAVGVILKYWLIDDLYLEPYGRAFLNNLSGTPQYLNWMTAVIRPHMGNSVLEIGAG
                     IGNLSARLMGRRSRYVAADHDPLYLHTLQNRFLRTPNVSVAKIEPSNSQDFIPFRQQF
                     DTALCLNVLEYAASPASVLHSLAASLVPGGRLIVLVPQSPSLYGTIDKTLGHRRRYCL
                     SELSKELEGCGLMVEHVQHLNKIGTPAWWIYARLLRRTGIDKLTLKLFDITVWFWRRV
                     DRILPWRGLSLVVSARKRA"
     gene            4299..4577
                     /locus_tag="VFQ79_17665"
     CDS             4299..4577
                     /locus_tag="VFQ79_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141556.1"
                     /translation="MEVSAVSPAAADRVRGRIGTVVDRRGRIELFEVQGQLVGAGYAR
                     GIERGVQAMSARVAAGSRKPDVKRCGPKVKQAGRRAFAFVVAACSRSF"
     gene            complement(4649..5020)
                     /locus_tag="VFQ79_17670"
     CDS             complement(4649..5020)
                     /locus_tag="VFQ79_17670"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEU0141557.1"
                     /translation="MTKRVFVACAALAVLAAGQLFAAAPTKPKSVIHVVTVQWKPDAS
                     KEQIQQAIAGVENMNFPGLKNVWTRPIKTQLPEGYTSVIVMEFESEDALKKYAGSDAQ
                     KQWYKVYLPIRGESRTHDISN"
     gene            complement(5106..6041)
                     /locus_tag="VFQ79_17675"
     CDS             complement(5106..6041)
                     /locus_tag="VFQ79_17675"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEU0141558.1"
                     /translation="MREKLSSTVPADEYFMRLALEEARVGDRTPGAGEVGCVIVRGGE
                     VAASGHNEVEMRNDPTAHAEVVTLRRLGEKLKTIDFSGCTLYSTLQPCGMCTMAAIWS
                     RIGRIVYGATRSDVHSMYFEERHYNTADFIRDAFEADIEMTAGVLAAECSQLYYTPGE
                     LPGDARDDTAGAKPDEEAPVVGGIVETALYVADLDRSERFYTRLFGFERMYAEQDRMR
                     ALAIPSHQVLLLFRAGASVAGSSGNILGHDGRGNLHVAFSIPREQVALWERRLAGSGI
                     PLESKVNCGDGISLYFCDPDRHLIELITPGCWRNY"
     gene            complement(6070..>7176)
                     /locus_tag="VFQ79_17680"
     CDS             complement(6070..>7176)
                     /locus_tag="VFQ79_17680"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU0141559.1"
                     /translation="ELRAKGHTEGVAYDGYILDFLVHWLEVIPRDAADRILKHPAFPE
                     YLEQSYMLSAPGAIEQVAELSDVEPKEMPFHYSAQAKLARFGLNPVLAWFLGGWPVDW
                     IRADALSVLAPLAGRLDGTMPPPGALNAHYAAVLRSGWGEKDAAVVMSATPSPTGHMQ
                     ADNGTVVIGSRGRWLISDPGYQQYMRDVEREFTIGPAAHNYPLINGAAQDSKKPGPVA
                     LDTVAPGLLRARVELAGCYPAAAQAKSVVRTAWLHSNSTVVIADQVDAAGLESLTYHW
                     HGHPEAAWGHQAGWLRLYFPDVDLWFTAPASPLSDSQVTRHPGSRGQLTATARIAPPP
                     PVMWWIFSTSPAPPEYKLDSSGKELQVQGVRFRV"
BASE COUNT         1451 a         2216 c         2056 g         1453 t
ORIGIN      
        1 gacgccgtgg gcatcggcac ggacttcgac ggcgtcagtt gtgttcctgt tgggttcgag
       61 gacgtctccc gatttcctgc tctcgaaaag gcgctcctcg ggaagggata ttccgccgcg
      121 cagatcggca aaatcttcgg cggaaacatt ctgcgagtca tgcgtagcgc ggagcaggct
      181 cgcggaagtc cgagcaatta gttaccgggc cgcgaccgcc gggaagcggt tattcaggag
      241 gggagatgac aaggcgggag tttgtttcag cagcagcagc cgtctcctcc tccccggcgc
      301 ttctggtaat accggttcat caggtgatcg atgcacgcgc aaagcatacc cccgagcagc
      361 ttcaccgctt ctcatcgggc atctggacgg aggcggtccg ggacttccgg cgatgcggta
      421 ttcagttcca aggtacccag gggccgggag aaatccggcg ctctcccggc ggaagacctg
      481 tattcaacgg gctgcgggat ggcatgatta acctgatcct gaccggccac cttcccatgg
      541 cgtgggacaa aggccgcgcc ctgccgggtt tgtcgacccg gcacgaaggg cgtcccatat
      601 gcataattgc gctccggtat gcgcatggcc atcaaatccc gttcgtttcg gtcaacacct
      661 gcgtgcacga acttctacac gtgctgttgg gagacatttt cgaaagccgc cccaaaggat
      721 tgccgggctt cggacgtgaa cttcgaatcg attggtatgc cacccggctt tggctcttcc
      781 gtgatggcgc cgctatccgc gaagcggccc gggcctatct cgaccgtttg cggaccgagt
      841 tggctgcgcg cacgtgacgc gcctacgccg ccacggactg ctctttgtac cagtgcaggg
      901 ttctcttcag tccttcttcg atcgagaacc ggggcgagtg gccaagatcg cgggcggccg
      961 ccgccgtatc ggcttgcgag tggcgcacgt cgccttccct cgatgggccg tagctggccg
     1021 ggagagacac gccttccaac ccctgcacca gcttccaaat ttcattgagc gtgtaccggt
     1081 tgccgttgcc ggcgttgtac acgcggccgc tcacgccctt caccgccgcc ctgacggtca
     1141 gcgcagcgac atcttccacg tatgtgaaat cccttgactg ctcgccgtcg ccgaagatga
     1201 caggcgaacg tctctccgcc acgcaccgca taaagattga aagcactccg gagtacggac
     1261 tcgatggatc ctgccttggc ccatacacat tgaaataccg gagcgccacg gtctccaggt
     1321 tgaaacactt cgaaaaaacg ctacagtaat actcgccgaa aagcttttgc gcggcgtaag
     1381 gagactgcgg attaggcacc attgtctcga cttttggcaa cacctcggta tcgccatagg
     1441 cggacgacga agcggcatac accaccctgc gcgcacccgc atcggccgcc gcccgcaata
     1501 gctgaaacgt gccgtccacg ttcacttcgt gcgaaggcac cgggtcttta atcgaacgtg
     1561 gaacggaggg aatggccgcc aggtgaaaaa cggcgccggc gccctccagg agcggccgga
     1621 tctcgtcata gtgccggata tcggcaagat ggaaatcgac ccggtctttg acttcgtcca
     1681 ggttttcgcg tttcccactc agtaaattat cgatgaccgt caccctgggc gcgccgtcct
     1741 tcagaagtgc cctcaccacg ctggagccga taaatcccgc tcccccagta acaacaaact
     1801 tgctcattat gccaacctcg aaatttcgga ttctatatat cgaagagatt cgttcgcgat
     1861 cttctcagcg cccggactga aatcgaatac ttccagggac acccagccct ggtagttccg
     1921 attcttcagc acctggagca caggtttaaa atcgtaggat cccgtgcccg gatgccggcc
     1981 gtccatctca ttgatgtgaa catgccggat cagctcgaaa taccggtcga cgatcgtcgc
     2041 gtgctcctcg gcttcatcga ccgcattatg cgtgtcgaac atggtgcgga tggccgggct
     2101 gttaattccc ttcacgattt ccaccgcttc ggaaacgctc tgcaccacat cacattgggc
     2161 cctcggcaac gcttccacga gaatggttac tccccggccg gcggcctgcc cggcaacttc
     2221 ggtcaaacct tcgatgaaat ttcgcgtggc ctcgggcgcc gtcaaccgtc ccgtggtgga
     2281 ccgctgctgc ggcgaaccaa acaccatcac gccgtccggc ccaagatccg cgcacaggtc
     2341 gatgaggact cgaatgtgct gccagctctt ctcacgtagc ttcttatcgg ccgtggtgac
     2401 atgaagccct ttaggagaaa ccatcagcca gtgcagcccc acaaacgtga gtccttcgga
     2461 tgccatcaca tcccggtatt ctttccgggc cgcggccgta atctttgccg ggtcttccgc
     2521 cagcgtgaat ggggcgatct cgatcccctg atatcccgcc ttgcgaatcg ctttgcaggt
     2581 atcggaaaag gaccagtctt tgaagacctc gttgcatatg gactgcttga attggtaact
     2641 catgcgctat ctcagtgtag tcgggtgaat caggcgggac aatcccggtt aagctcgttt
     2701 gcgcgcgctg acgaccagcg aaagccccct ccagggaagt atccggtcga cgcgccgcca
     2761 aaaccacacg gttatatcaa acagcttcag ggtcagtttg tcgattcccg ttcttctgag
     2821 cagccgtgcg taaatccacc acgccggcgt gccaatttta ttcaaatgct gcacgtgctc
     2881 aaccatcagg ccgcaacctt ccaactcttt cgaaagctcg ctgaggcaat agcggcggcg
     2941 atgcccaagc gtcttgtcta ttgtcccgta aagtgagggg gattggggta ccaggacgat
     3001 cagacgccct cccggtacca ggctggcagc cagggaatgc agcacggaag cgggagacgc
     3061 cgcatactcc aacacgttca ggcagagtgc ggtgtcgaac tgttgccgga aagggatgaa
     3121 atcctgtgag ttggaaggtt cgattttagc gacggaaacg ttcggcgtgc gaagaaaccg
     3181 gttttgcagc gtatggagat agaggggatc gtgatccgcg gcgacatatc tggaacggcg
     3241 gcccatcagg cgcgcggaaa gatttccgat acccgcgccg atttcgagaa ccgagtttcc
     3301 catatgcggc cggattacgg cggtcatcca gttcaggtac tgcggcgtgc cggacaggtt
     3361 gttcagaaac gcgcgtccat aaggctccag atacaggtcg tcgataagcc agtacttcaa
     3421 aatcacgccc accgcgttca gcccatcccg ccaccggatt tttttgccct cttcatacgt
     3481 gcggccgtga taactgatag gaacctcgta aatgcgaaag tgcctctttg cacatttcac
     3541 ggtgacttcg ggttcgaagc caaaacggtc cgaccggatc ggaatacttt tcagcagatc
     3601 ggtgcgaaag actttgtagc cggtctccat gtccgaaata ttctgattgc agaacatgtt
     3661 cgatacgagt gtcagaccct ggttgattac cgaatgccaa aacggcagca cgcgcgtctg
     3721 ttctcccgcc aggtaacgcg atccgaacac tacgtccgcc cgtccgtcga ggagtggcct
     3781 caggagcctc gaatactccg atggatcgta ctccaggtcg gcgtcctgaa tgatcgagaa
     3841 gtctcccgtc gcctgctgca cggcagtgcg tatggcggcg cccttacctt tattcaccgg
     3901 gtgccggaac aggcggatct ccgggtgctc cgacgccaac cgctcgagga ttccgaacgt
     3961 gccgtccgtg gaacaatcgt ccaccagaat cacctcgcgg tcgaggtttt cagggagcgg
     4021 agtggaaaga acctgcttga gacaacgctc cacgaccact tcttcgttgt aaacaggaac
     4081 cagaacggat agtcgcacgg aacagaatcc cagtatagta gttccccgct aatgcgcccg
     4141 aaccgctgcc ggcggtgcgc tgattacctg gggaagctcg ggcggaaacc cgtggtcatg
     4201 gccgctactc ccaatcttcc gcccgagaca aaacggaaat tcaggttctg gaactggtcc
     4261 acctcatagt cgttgaacgc gtttcggaac aggcgcggat ggaagtttct gctgtaagcc
     4321 cagccgccgc ggatcgcgtc cgcggtcgca tcggcacggt cgtcgatcgt cgcggtcgca
     4381 tagagctgtt tgaagtacag ggtcagctcg tcggcgccgg ttacgcgaga ggcatcgaaa
     4441 gaggtgttca ggctatgagt gcgcgcgttg ccgcgggcag ccgcaagccc gatgtcaagc
     4501 gatgcggtcc aaaggtaaaa caggccggca ggcgcgcttt cgccttcgta gtcgcggctt
     4561 gttcccgatc gttctgagtt ctttcggtac ggctatgaac gaagaccggg cggcttttgg
     4621 gcaaccgccc ggaacagaga aggcgaggtc agttgctgat atcgtgagtg cggctttcgc
     4681 cgcgaatagg caggtacacc ttataccact gcttttgagc atcgctgccc gcatacttct
     4741 tcagggcgtc ttcgctttca aactccatca cgatgacgct cgtatagcct tcgggcaact
     4801 gcgtttttat gggccgggtc cagacgttct tgagacccgg aaaattcatg ttttcaacgc
     4861 ccgcaatggc ttgttgaatc tgctccttcg acgcgtccgg tttccattgg acggttacaa
     4921 catggatcac ggactttggt tttgtgggcg cggcggcaaa caactggccg gcagccagga
     4981 cagcaagcgc ggcacacgcc acgaatacac gtttggtcat ggttgtcgtc ggttcctcct
     5041 ctgtaaagtc gtcgggttga ctctctctat gttaaccgta tccggccgcc tttcgaggga
     5101 gacgctcaat aatttctcca gcatccaggc gtaatcaact cgatcaggtg ccggtccgga
     5161 tcgcagaaat agaggctgat accatctccg cagttcacct tgctttcgag cggaatacca
     5221 gagccggcaa gacggcgctc ccaaagcgcc acctgctccc gcggtatgga aaaggccacg
     5281 tggaggttgc ctctcccatc gtgaccgagg atgtttccgc tggagcccgc tacggaagcg
     5341 ccggcgcgaa acagcaacag cacctggtgg ctgggtatcg cgagcgcacg catgcggtcc
     5401 tgctccgcat acatcctttc aaagccgaac agccgcgtgt agaaccgttc gctgcgatcg
     5461 agatcggcga catagagggc tgtttccaca atgcccccca caacgggagc ctcctcgtca
     5521 ggcttggcac ccgccgtatc atcgcgcgcg tcaccgggca actcaccggg cgtgtagtaa
     5581 agctgggagc actcggcggc caggactccc gcggtcatct cgatgtcggc ttcgaatgcg
     5641 tcgcggataa aatcggcggt attgtaatgc cgctcctcga agtacatgga gtggacatcg
     5701 ctgcgagtgg cgccatagac gatacgcccg atccgggacc agattgccgc catcgtgcac
     5761 atcccgcaag gctgcaatgt cgaatagagc gtgcatccgg agaagtcgat cgtcttcaac
     5821 ttctcgccaa gcctgcgaag cgtgacgact tcggcgtgcg ccgtcggatc gttccgcatc
     5881 tcaacctcat tgtggccgga tgcggctact tcgccgccgc ggacaatgac acagcctact
     5941 tcgcccgcgc ccggcgtgcg gtctccaacc cgggcctcct cgagcgccag gcgcataaag
     6001 tattcatcgg ccgggacagt ggaagacagt ttttcccgca ctctgcactc attatccccc
     6061 gcggtgaaat tacacccgga accgcacccc ctgaacctgt agttccttac ccgagctgtc
     6121 cagcttatat tccggcggcg cgggcgaagt cgaaaaaatc caccacatca ccggcggtgg
     6181 cggagcgatt ctggcggtgg cggtgagctg gccgcgcgag ccggggtgcc gggtaacctg
     6241 ggaatccgac agcggtgaag caggcgctgt gaaccacaga tccacatccg gaaaatacag
     6301 gcgcagccag cccgcctgat gtccccatgc cgcttcggga tggccatgcc agtgataggt
     6361 gaggctctcg agccctgccg cgtcgacctg gtccgcaatc accaccgtgc tgttcgaatg
     6421 aagccacgcg gtgcgcacca ccgatttcgc ctgtgccgcc gccggataac agccggcaag
     6481 ctcgacccgc gcgcggagca gtcccggagc aaccgtgtcc agcgctaccg gacccggttt
     6541 cttgctgtcc tgcgccgcgc cgttgatcaa cggataattg tgcgccgccg gcccgatggt
     6601 gaactcgcgc tcgacatccc tcatgtactg ctggtagccg ggatcggaga tcagccagcg
     6661 gccgcgcgat ccgatcacca ccgtcccgtt atccgcttgc atgtgtccgg tcggcgaggg
     6721 cgtggcggac atgacaaccg ctgcatcctt ctctccccag ccgctccgca gcaccgcggc
     6781 ataatgagcg ttcaacgctc ccggcggcgg catggtgccg tccagccggc cggcgagcgg
     6841 tgcgagcacg ctcaaggcat ccgcacgaat ccagtccacc ggccagccgc ccagaaacca
     6901 ggcaagcacc ggattcaaac cgaagcgggc gagctttgct tgtgccgagt aatggaaggg
     6961 catctctttc ggctcgacat cgctcaactc agccacctgc tcaatggctc ccggagccga
     7021 gagcatgtag gattgctcca ggtattcggg gaatgcaggg tgttttagaa tcctgtccgc
     7081 cgcatcccgg ggtatcactt ccagccagtg cacgagaaaa tcgagaatgt atccgtcata
     7141 agcgacgccc tcggtgtgcc cctttgcccg aagctc
//
LOCUS       DASTMK010000411         8787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_533797, whole genome shotgun sequence.
ACCESSION   DASTMK010000411 DASTMK010000000
VERSION     DASTMK010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533797"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFQ79_17685"
     CDS             <1..893
                     /locus_tag="VFQ79_17685"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU0141560.1"
                     /translation="NGVSTRDMPLAFAELLLQGAAGSSVEISVMRLRRSEPQKITLAR
                     EVLKYPAINTKMLNGQIGHVQVESLETGKTKQIAAAVTQLEKQGAAKIILDLRNNAAG
                     STEEGIAVANLFLEKGLITYLQGQKYPRRDFTADAQVAIYREPLVVITNRGTAGGAEL
                     AAAALLGNKRAEIVGERTYGDAAVRKTVPMDDGSAVVLSVAKYYSPDGKAIQDTGVTP
                     TVAVAEMEAAAEVPGEEAPQTQPEAKPEEDILLKRALEVATKGAPPAGSETAAPAAVD
                     PQGGIPPRDLPPDSRPEEKK"
     gene            906..1205
                     /locus_tag="VFQ79_17690"
     CDS             906..1205
                     /locus_tag="VFQ79_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU0141561.1"
                     /translation="MPLYEYKCKSCGDVFEVIQKFSEAPLTSHNGCGGAVERLLSAPA
                     LQFKGTGWYITDYARGNGNSKENPTKSDSRSDSKTSESASKSETPSAPAVSAEKK"
     gene            1291..2367
                     /locus_tag="VFQ79_17695"
     CDS             1291..2367
                     /locus_tag="VFQ79_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU0141562.1"
                     /translation="MPHWILLPVVLLGLVSPTNAIERPGVEFKVFQFPPNAIPRIDGD
                     TADWDLVPPGYAIGTDQLRDTVGNQSFNPKDLDVRVRVGWVKGLNRLYFLYEAYDDYW
                     DFSRSDLHNDIFEVVVDGDLSGGPFIKQMHPYKALNVWDAHFLFHGVHAQNYHIFTPA
                     EGKDWAMVWGCQPWIKELPWANAAYSHQLKPGGSGRLVLEFWITPFDYAGCEGPARAV
                     PSELWENKVIGISWSVLDYDDSKSERYKGFWNLSHKTTMYGNASDAVAFRLMPLESKF
                     RKPIEAQWSFHVLDMARRAVAFKDLSHGDISAWRWDFGDGKTSNEQHPVHVYEKPGEY
                     VVVLEVEGPAGSSKRAKVWDVSLK"
     gene            2447..3619
                     /locus_tag="VFQ79_17700"
     CDS             2447..3619
                     /locus_tag="VFQ79_17700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEU0141563.1"
                     /translation="MKAVCWYGKRELHVEEVPDPVILNPRDAIVKVTLTAICGSDLHL
                     YNGFIPTMKKGDILGHEFMGEVVDVGKGISTLKVGDRVVVPFPIACGNCFACHDEMYS
                     LCENSNPNAWIAKKLWGHSPAGIFGYSHMVGGYAGGQAQYVRVPFADVGPIKVPDGLS
                     DEQVLFLSDILPTGYMAAEACQIQPGQTIAVWGCGPVGQFAIKSAYLLGARRVIAIDR
                     FPERLAMARDKGGAEIINYEQEDVYDSLLAKTGGRGPDACIDSVGMESHAPGVVGAYD
                     RTKQAMMLQTDRTGALRQAIMACRNGGTVSIPGVYGGFADKFPLGSLMNRSLTIKSGQ
                     THVQRYMRPLLERIEKGEIDASFVITHRLGLSEAPAAYEMFKNKQDGCIKVVMKPQ"
     gene            complement(3624..4934)
                     /locus_tag="VFQ79_17705"
     CDS             complement(3624..4934)
                     /locus_tag="VFQ79_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141564.1"
                     /translation="MVPWAAAVLLLTSWFEEGLALAKHGETQAAIGRFQVAVKLEPER
                     PAAWFNLGAMLHRAGDMDGAVAALEKAVRLDSRDPEPLNALGLIHEQQGDDKEAVQCF
                     RAALRLKPGYLEARHNLGVALVSSRKLEEAVAELRAVIAAQPDWAEPHLTLARAYHRM
                     NRAKEAISEYRTALRWNPSLAAAAFHLAQLLDQQQNDAEALKQYRAAAAMEQDNVGYR
                     YALARALQRSGHADEAREHFEATAKLRAQQRAGELADTANRAGVAAAESGNLEAAITH
                     FRRAAEYLPKAAEAFYNLGGVYLRQHALDQAAQSFRRAIELEPEFLAARTELASVLVA
                     KENWNEAAQQYRAVLKLRPGDLDLRYRLADVLAQSGDHADVVRELQLASDIDPDNAAV
                     QYNLGVALLNTGDTAGAAQRFERALTLDPGNAKARAVLDSIRER"
     gene            4957..5985
                     /locus_tag="VFQ79_17710"
     CDS             4957..5985
                     /locus_tag="VFQ79_17710"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141565.1"
                     /translation="MLLEVGIAVLLAPATAQSHFARGVELREAGRKIEALREFRRAAE
                     LDAAMAAAHREIGMLLLDQRDFAGAADAFERAVAINPGDMDSRYNRAMSLANAGRRTT
                     AIRELEALLKEKDDWGQAWFGLGHVYARAGRTNEAERAFRKSITLDPGLFRAHFELGR
                     LLDDKGDREGAMEAFAAGVKLAPDSTSARFRLATLLQQAGRREEAAHHFAAVRELRDR
                     RQRGEQAALAYQQGLKYLQAGDDASAIRELKRAVELRSDFPESRIALGEAYEHSGDLA
                     AAIVEFQRALELAPSAETANHAGVLLAKSGRIDESIRAFRRALEIDPNFANADKNLKQ
                     ALQIQKRR"
     gene            6004..7569
                     /locus_tag="VFQ79_17715"
     CDS             6004..7569
                     /locus_tag="VFQ79_17715"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0141566.1"
                     /translation="MKLLYPIALLASAAIMPAAESFDVVVYGATSGGVTAAVSAAREG
                     AKVALIEPGRHLGGMSSGGLGATDYGKQETIGGLSREFYEGLGRHYGKEIAWTFEPHV
                     AENVFKEMVKEAGVKVYYEHRLKEKGGVKKSGARITEIVMENGKVFRAKAFLDCTYEG
                     DLMAFAGVSFTWGREGTDQYGEEFAGVRPVDRYHHHIFPPGVSAYDENGKLLPEISPE
                     PRGELGAADKKVQAYNFRICLSKDKNNQVPFTKPANYDPHRYALLLRFIEAFEKHNGR
                     PARLKGDLLIWSLMPNNKTDINNRGGFSTDYIGKNWEYPTANYKHRAEIWQDHLNYTA
                     GFFYFLANDPRVPQAVRDDLNEWGLAKDEFADTNHWPHQLYIREARRMIGDFVMTEKD
                     ARTDLVKEDSVAMGSYQIDSHNIQRHVTPEGFVQNEGDTEVPAKPYQIAYRVMLPKKN
                     EASNLLVPVCVSSSHIAYGTVRMEPVFMALGHAAGIAAHMAATKNVTVQEIDVPALRA
                     KLRAQRAVISLTN"
     gene            7591..>8787
                     /locus_tag="VFQ79_17720"
     CDS             7591..>8787
                     /locus_tag="VFQ79_17720"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141567.1"
                     /translation="MRFRFDPALVLLIFLISTGLMFAQSPLGTITGTISDTQNARVPG
                     VEVTATQVGTGLSFKATSSGDGTYVIPNLPVGRYEVEASATGFKSFRRTGVVLEVSQR
                     LRLDIALELGALTETITITGAVSRVQTEEATLGSVVERQRIEELPLNGRHVFNLVKLV
                     AGVRPVDRAADGFAEISNQGFSQMTFNGGPVYGNQFYVDGGMNTVPVHNEISVVPMTD
                     AVEEFKVETNSLKAEFGQTSGGVVNVVTKGGTNEIHGSLYEFFRNDSLDARHAFATQT
                     DPLTGRIKPILRYNQFGGTVGGPVYLPKLYDGRNRTFFFVGYEQWRQSSSSLRRSTVP
                     TALERAGDFSRTFDSRGSLIPIYDPATTIQNPNGSGFVRQPFAGNVIPSSRFDPVSLK
                     VMEFMPL"
BASE COUNT         1848 a         2511 c         2661 g         1767 t
ORIGIN      
        1 tcaacggcgt ttccacccgc gatatgccgc tggccttcgc cgaactgctg ttgcagggtg
       61 ccgccggttc gagcgttgag atttctgtga tgcggctccg ccggtcagag ccgcaaaaga
      121 ttacgctggc gcgggaggtg ctgaaatatc ctgcgatcaa taccaaaatg cttaacggcc
      181 agatcgggca tgtgcaagtg gagagcctgg agacagggaa aaccaagcag atcgccgcag
      241 ccgtgaccca actcgaaaaa cagggcgcgg cgaaaatcat tctcgatctg cggaataacg
      301 ctgccggcag cacggaagaa ggcattgcgg tggcgaatct ttttctggaa aaaggcttga
      361 ttacctacct tcaggggcag aagtatcccc ggcgtgactt cacggccgat gcacaagttg
      421 cgatctatcg ggagccgctg gtggtaatca ccaacagagg cacggccgga ggagctgaac
      481 tggccgccgc cgcccttctg ggaaacaagc gggcggaaat cgtgggcgag cgcacttacg
      541 gcgacgccgc cgtacgcaag accgttccga tggacgatgg aagcgctgtt gtgctttccg
      601 tcgccaagta ttattctccc gacgggaagg cgattcagga taccggtgtt acaccgaccg
      661 ttgcggtggc ggaaatggaa gcggcggctg aggtgcccgg cgaagaggct ccacaaactc
      721 aaccggaagc caaacccgaa gaggacattc tgctgaagcg cgctctggag gtagcaacga
      781 aaggcgcacc accggcgggt agcgaaaccg ccgcccccgc cgctgtggat ccacagggtg
      841 gaattcctcc ccgcgatctt ccccccgatt cccggccgga agaaaagaag tgagttgaac
      901 tgtatatgcc tctttacgag tacaagtgca agtcctgtgg cgatgtcttt gaagtaatac
      961 agaaattttc cgaggcccct ctcacgtcgc ataacggatg cggcggagcg gtggagcggt
     1021 tgctgagcgc tcctgccctc cagttcaagg gaacgggctg gtacatcacg gactatgccc
     1081 gcggaaacgg gaattcgaaa gagaacccca ctaaatccga ctcgaggtcc gactcgaaaa
     1141 ccagcgagag tgcgagcaag tccgaaactc catccgctcc cgctgtttcc gccgaaaaga
     1201 aataggctct acttttgagc agaccgctcc ctggcggtca cggctcagaa acggagacgc
     1261 gtgccgtgat atgctcgccg cggaggtggc atgccgcact ggattcttct tccggttgtg
     1321 cttctggggc tcgtgagtcc aaccaacgct atcgagcggc ctggtgtcga gttcaaagtg
     1381 tttcagttcc ctccgaatgc cattccccgc attgacggcg acactgccga ttgggacctg
     1441 gtgccgcccg gctatgccat cggcaccgat caattgcggg atacggtggg caatcaatcg
     1501 ttcaatccga aagacctgga cgtccgggta cgcgtgggtt gggtgaaggg cttgaaccgg
     1561 ctctatttcc tctatgaagc ctacgacgac tattgggatt tctcgcgcag cgatttgcac
     1621 aacgacattt ttgaagtggt tgttgacggc gatctttccg gcggaccctt catcaagcaa
     1681 atgcatcctt acaaagcgct gaacgtttgg gatgctcatt tcctcttcca cggagtgcat
     1741 gcgcagaact accacatttt cacgccggcg gaaggcaagg actgggccat ggtttggggt
     1801 tgccagccct ggatcaagga attgccatgg gctaacgcgg cttattcgca ccaactcaaa
     1861 cccggtggga gcggcagact ggtgctcgaa ttctggatta ctccattcga ctacgccggc
     1921 tgcgaaggtc cggcacgcgc tgtgccttcc gagttgtggg aaaacaaggt gattggcata
     1981 tcctggtccg tgctggacta cgacgattcc aagtcggagc ggtacaaagg cttctggaac
     2041 ctgtcgcata agaccacgat gtacggaaac gcttccgatg cggtggcttt ccggctgatg
     2101 ccgctcgagt cgaagttccg caagcccatc gaggcgcaat ggtcctttca cgtgctcgat
     2161 atggcacgcc gggccgttgc gttcaaggac ctgtcgcatg gcgacatcag cgcctggcgc
     2221 tgggatttcg gcgacgggaa aacttccaac gaacagcatc ccgtacacgt ttacgagaag
     2281 ccgggcgaat atgtggtggt attggaagtg gaaggacccg ccggcagcag caaacgcgcc
     2341 aaggtgtggg atgtttctct gaagtaagta tcacacccgt tccgtcgcgg ttctacaacg
     2401 aaacgttttg ccggcagagc gaatccactt tccgaagtga gaacgcatga aagctgtctg
     2461 ctggtacggt aagcgcgaac tgcacgtgga agaggtgccg gatcctgtca tcctcaatcc
     2521 gcgcgatgca attgtcaagg tcactctgac cgcaatctgc ggctccgatc tccaccttta
     2581 caacggcttc attcccacca tgaagaaggg cgacattctg ggtcatgagt ttatggggga
     2641 agtggtggat gtggggaagg ggatctccac tttgaaagtg ggtgaccggg tagtagtgcc
     2701 gtttcccatt gcctgcggaa actgcttcgc ctgtcatgac gaaatgtatt cgctttgcga
     2761 aaattcgaat ccgaatgcat ggatagcgaa gaaactatgg ggacattcgc cagcgggcat
     2821 cttcggctac tcgcacatgg ttggggggta tgccgggggg caagcacagt atgtccgtgt
     2881 ccctttcgct gacgtcgggc ctattaaagt cccggacggc ctttccgatg aacaggtgct
     2941 cttcctatcg gacatcctgc ccaccggcta catggctgcc gaggcttgcc agattcaacc
     3001 gggacagacc atcgccgtct ggggctgcgg tcccgtcgga caattcgcga tcaaaagcgc
     3061 gtaccttttg ggagcccggc gcgtgatcgc cattgaccgc tttcccgaac gtctggcgat
     3121 ggcgcgtgac aaaggcggcg ccgaaatcat caactacgaa caggaagatg tctacgattc
     3181 gctgctggcc aaaaccggcg gacgcgggcc ggatgcctgt atcgattcgg tagggatgga
     3241 atcgcatgcg cctggagtgg taggagctta cgatcgcacc aagcaggcga tgatgctcca
     3301 gacagaccgc acaggggcgc tacgtcaagc catcatggcc tgccggaacg gtggaaccgt
     3361 ttcgattccc ggtgtttatg gcggctttgc tgataaattt ccacttggct cgttgatgaa
     3421 ccggtcgctc accatcaaga gcggacagac gcatgtgcag cgctatatga ggccgctgct
     3481 cgaaaggatc gagaaagggg aaatcgacgc ttcgttcgtc attacgcacc ggctggggct
     3541 aagcgaggcg ccggcggcat atgagatgtt caaaaacaag caggacggct gtatcaaggt
     3601 agtgatgaaa ccgcaatagg cgattagcgt tcccggatgc tgtcgagcac ggcgcgagct
     3661 tttgcgttgc ccgggtcgag ggtgagtgcg cgttcgaacc gctgtgccgc gcccgctgtg
     3721 tcgccggtat tcagcaatgc gaccccgaga ttgtattgaa ctgccgcgtt atccgggtcg
     3781 atgtccgacg cgagttgcag ttcccgcact acatcggcgt gatcgccgga ttgcgccagc
     3841 acatccgcca ggcgatagcg aaggtcaaga tctcccggcc tgagcttcag aaccgcccta
     3901 tactgttgcg ccgcctcgtt ccagttctcc ttggccacca ggacggacgc cagttccgtc
     3961 ctcgcggcga gaaactccgg ctcgagttcg atggctcgcc ggaaggattg cgcagcctgg
     4021 tccagggcgt gttgccggag atataccccg cctaagttat agaacgcttc cgccgccttg
     4081 ggtaaatatt ctgccgcccg ccggaaatgg gtgatagcgg cttcgagatt tccactttcg
     4141 gctgccgcca cacccgcgcg attcgcggtg tcggccagct cgcccgcccg ttgctgtgca
     4201 cgtaacttgg ctgtggcttc aaaatgctcc cgcgcttcgt ccgcatgccc ggaacgttgg
     4261 agcgcccggg cgagagcgta ccggtatccc acattgtcct gctccatcgc ggccgcggca
     4321 cgatactgct tcagcgcttc cgcatcgttc tgctgttgat cgagtaactg ggcgaggtgg
     4381 aaggccgccg ccgcaagact cgggttccat cgcagcgccg ttctatattc gctgatcgct
     4441 tcctttgcgc gattcattcg atgataggcg cgcgcaagcg tcaaatgagg ttcggcccaa
     4501 tcgggctgcg cggcgatcac cgcccgcagc tccgcgacag cttcttcgag tttgcggctc
     4561 gacaccagcg cgacgccaag gttgtgccgg gcctccaggt agccgggttt gagacgtaac
     4621 gcggctctga aacactgaac cgcttccttg tcatcaccct gctgttcatg aatcaagccc
     4681 agggcgttca gtggttcggg gtcacgactg tcaagccgca ccgctttctc gagtgctgcc
     4741 accgcgccgt ccatgtcgcc ggcgcggtgc agcatagcgc cgagattgaa ccacgcggcc
     4801 gggcgctccg gttcgagttt cacggcaacc tggaaacggc cgatagccgc ttgcgtctcg
     4861 ccgtgtttcg caagtgccag accctcttca aaccaggagg tgagcagcag aaccgcggcg
     4921 gcccagggca ccatgagtgc agtatagttg ttctaagtgc ttctggaggt agggatcgct
     4981 gtgctgcttg cgccagctac tgcgcaatcg cattttgcgc gcggcgttga actgcgcgag
     5041 gcgggccgga aaatcgaagc cctgcgtgag tttcgccgtg ccgcggagct ggacgcggct
     5101 atggccgcgg ctcaccggga aatcggcatg cttctgctgg accagcgcga ttttgccggg
     5161 gccgcagacg ccttcgaacg cgccgttgcg atcaatcccg gcgacatgga ttcccgctat
     5221 aaccgggcca tgtccctggc gaacgcgggg cgccgcacca cagcgatacg cgaactcgaa
     5281 gcgttgctca aagaaaagga cgattggggc caggcatggt tcggcctggg ccatgtatat
     5341 gctcgcgccg gccgcactaa cgaggccgag cgcgccttcc ggaaatcgat tacgctcgac
     5401 cctgggctgt tccgcgcgca ttttgaatta ggcagattgc tggacgacaa gggagatcgc
     5461 gaaggagcga tggaagcctt cgcggccgga gtgaaattgg cgcccgattc gacgtcggcg
     5521 cggtttcgtc tggcgacgct gctccagcag gccggccgcc gcgaagaggc ggcgcaccac
     5581 ttcgccgccg tgcgggaact gcgagaccgg cggcagcgcg gagagcaggc agcgctggca
     5641 taccagcagg gtctgaaata cctccaggcc ggagatgacg cctccgctat ccgcgaactg
     5701 aagcgcgccg tggagttgcg ctccgatttt cccgaaagcc gtatcgcgct gggagaggct
     5761 tacgagcatt ccggcgacct ggccgcggct atcgtggagt ttcagcgtgc tctggaactc
     5821 gcaccttcgg cggagacggc caatcacgcc ggcgtcctgc tggcgaagtc tgggcgcatc
     5881 gacgaaagca ttcgagcgtt tcgccgtgcg ctcgaaatcg atcccaattt cgcaaacgcg
     5941 gataagaacc tgaaacaggc gcttcaaatt cagaagcgcc gctgatgcag gaaaagaata
     6001 acgatgaaac tcctttatcc aattgctcta ctcgcctccg cggcgattat gccggccgcg
     6061 gaatctttcg atgtcgttgt ttatggcgcc acgtccggcg gtgttacagc agcggtgtcc
     6121 gctgcgcgtg aaggtgcgaa agtcgcgctg atcgagccgg ggcggcacct cggcggcatg
     6181 tcttcgggtg ggcttggcgc tacagattac ggcaaacagg aaaccattgg cggcctgtct
     6241 cgtgagttct atgaaggcct gggccgccac tatgggaagg aaatcgcctg gacgtttgag
     6301 ccgcacgtgg cggagaacgt attcaaagag atggtgaagg aggccggcgt gaaagtttat
     6361 tacgaacacc ggctgaagga aaaaggcgga gtgaagaaga gcggcgcccg catcaccgag
     6421 atcgtgatgg agaacggcaa agtttttcgc gcgaaggcat ttctcgactg cacctacgag
     6481 ggcgatctga tggcgttcgc gggcgtctcg ttcacgtggg gcagggaagg cacggatcag
     6541 tacggcgaag agttcgccgg cgtgcgtccc gtggaccggt atcaccatca cattttccct
     6601 ccaggcgttt ccgcttatga cgaaaacggc aaattgctgc cggaaatcag tcccgaaccg
     6661 agaggcgaac tcggcgcggc ggacaagaaa gtgcaggcat acaacttccg catctgtctt
     6721 tccaaagaca agaacaacca ggtgccattt acgaaacccg cgaattacga tccgcatcgc
     6781 tacgcgttgt tgcttcgatt tatcgaagcc ttcgagaaac acaatggccg tccggcgcgc
     6841 cttaagggcg acctcctgat ctggtcgctg atgccgaata acaagactga catcaacaac
     6901 cgcggaggtt tttcaaccga ctacatcggc aaaaattggg aatatccgac cgcgaactac
     6961 aagcaccgcg ccgagatctg gcaggaccac ctcaactaca cggccggttt cttttatttc
     7021 ctcgccaacg atccccgggt gccgcaggcc gttcgcgacg acctcaacga atggggactt
     7081 gcgaaagacg agttcgcaga tacgaatcac tggccgcacc agctctatat tcgcgaggca
     7141 cgcaggatga ttggcgactt cgtgatgacc gagaaggatg cgcgcaccga cctggtgaaa
     7201 gaggactcgg tcgcgatggg ctcctatcag atcgattccc acaatattca gcggcacgtt
     7261 actcccgagg gtttcgtgca gaatgagggc gacactgaag tgcctgccaa accttaccag
     7321 atcgcctacc gcgtgatgct gccgaaaaag aacgaagcat cgaacctgct ggtgccggtc
     7381 tgcgtttcga gctcccatat cgcctacggg actgtccgga tggagcctgt gttcatggcg
     7441 cttggtcatg cggccggtat tgcggcgcac atggctgcca ccaaaaacgt aactgttcag
     7501 gaaattgacg taccggcgtt gcgcgcgaag ctccgcgcgc agcgtgccgt catctcactt
     7561 acaaactaac tttggatgga ggaggatgct atgcgattca gattcgatcc tgctttggtt
     7621 ctattgatct tcctgatatc gacggggctt atgtttgcac agtcccctct gggaacgatc
     7681 accggaacta tctccgacac gcagaacgcc cgtgtgccgg gagtcgaagt aacagccacc
     7741 caggtcggaa ccggcttgtc gttcaaagcg acaagttcgg gagatggaac atacgtcatc
     7801 ccgaacctgc cggtcggccg gtacgaagtc gaagcgagcg caacggggtt taagtctttc
     7861 cgccgcacgg gtgtcgtgct ggaagtttca caacgcttgc ggctcgatat tgcgctcgag
     7921 ctcggcgcgc tcactgagac catcaccatt acgggcgccg tctctcgtgt gcaaacggaa
     7981 gaagcgacgc ttggaagtgt cgtcgagcgg caaaggatcg aggaattgcc tctcaacggc
     8041 cgccacgtgt tcaacctggt gaagctggtt gccggcgtgc ggcctgtaga ccgcgccgcg
     8101 gatggattcg ccgagatatc gaaccagggc ttctcccaaa tgacgttcaa cggaggaccg
     8161 gtctacggca atcagttcta tgtcgatggc ggtatgaaca ccgtgccggt gcacaacgag
     8221 atctccgtcg ttccgatgac ggacgccgtc gaagagttca aagtggaaac gaactcactc
     8281 aaagccgagt tcggccagac cagcggcggt gttgtgaacg tcgtgacgaa aggtggaacg
     8341 aacgaaatcc atggttcgct gtatgagttc ttccgcaacg actcgctgga tgcccgtcat
     8401 gcgttcgcca cgcaaaccga tccgctgacg ggccggatta agcccatttt gcggtacaac
     8461 cagttcggtg gaaccgtggg tggcccggta taccttccga agttatacga cggaagaaac
     8521 cgtaccttct tctttgtcgg ctacgagcaa tggcggcaga gcagttccag cctgcggaga
     8581 tccactgtac cgacggcgct cgaacgtgca ggcgatttct cacggacgtt cgacagtaga
     8641 ggcagcctga ttcccattta cgatccggcc acgaccatcc agaacccgaa cggtagcggt
     8701 tttgtccgtc agcctttcgc cggaaacgtc attccgtcgt caaggttcga tcctgtgtcg
     8761 ttgaaggtga tggagttcat gcccctt
//
LOCUS       DASTMK010000412         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_180673, whole genome shotgun sequence.
ACCESSION   DASTMK010000412 DASTMK010000000
VERSION     DASTMK010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180673"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1195
                     /locus_tag="VFQ79_17725"
     CDS             71..1195
                     /locus_tag="VFQ79_17725"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0141568.1"
                     /translation="MPRSTVAALYTSPERVLDDIGRLAELAGMREALDARSTTILKDN
                     ISWHFPFPGANTTPWQLEGTILALRERGFKDLSCVQNKTVVTNAFKGEDLNHYVPVFR
                     RYEIPVFYNFHDADMKWIEYKPKGKMLVLDKIFREGIYVPDYFFGKNIVHLPTVKCHI
                     YTTTTGSMKNAFGGLLNTHRHYTHSWIHETLVDLLTIQKEIHAGLFAIMDGTTAGDGP
                     GPRTMRPKIKNLMLASADQVAIDAVAAKIMGFDPMSLPYIHLAHERGLGVGKPEEIHI
                     AGDDIRNESWGFSVGDNMASRIGDICWFGPLKRIQKLLFHSPLVHMFVFGSEAYHDWF
                     RWPVKDRRVYEQWLRTTGWGRLFGEYARYGTRTSAASAAD"
     gene            1304..2470
                     /locus_tag="VFQ79_17730"
     CDS             1304..2470
                     /locus_tag="VFQ79_17730"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0141569.1"
                     /translation="MMRVAIDATCWHNNRGYGRHARALVSTLVRQYPENEYRLFLDSA
                     GNTPDLAGHAKLELVAADTPASAAASSNGHRSLSDMWRMSRALSKARVDVLLYPTIYT
                     YVPALTRARKLVMIHDVIAETYPNLTLPRRTARLFWKAKVALGRMQADALVTVSEFSR
                     EKIIEHFRVAPERVFVVSEAADPVFRILEQPAMPASLRALGIDAGRRIVVYVGGFSPH
                     KNLEELMDAFSHIASLGQFDDCLLVLVGETRKEVFHSCLPALREKVDQAGLRDHVVFP
                     GYVSDEDLTELLNLSAVLVLPSFMEGFGLPAVEAAACGCPVIATKASPLPQLLEGGGI
                     YIEPGSGQLEQALEAVLASKEMRERMRRAGAAAAQRLTWNAAAEQLMQVMQRFE"
     gene            2479..3759
                     /locus_tag="VFQ79_17735"
     CDS             2479..3759
                     /locus_tag="VFQ79_17735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0141570.1"
                     /translation="MNFLHLTTFYPPYSFGGDAMYIYRLSHALAEAGHHVDVIHCVDS
                     YHLLHPAKPPIQFAEHPNVTRHELRSGHGWLSPFLTQQTGRPYLKTDTIRKVLSSRRH
                     DVIHFHNMSLLGPQTLAIEPPNPAAVKIYTTHEHWLVCPMHVLWKFNSRACEKPECLK
                     CTLLGKRPPQWWRYTGLLERAARRVDQFVSPSRFTAKMHASRGFSQPVEHLPYFIDRA
                     DDDWRTPSPRPQERPYFLFVGRLEIIKGLQTLIPLWKNVEFDLLVAGTGNYEVELRAM
                     AASNPRIRFLGPMSQRQLGALYHHALACILPSITYETFGMISIEAFARKTPVIVRDLG
                     ALPEAVEDSGGGFVYRSEEELLEAIRQIGASPGLRRELGEKGYRGFLEWWCREAHMRF
                     YFDYLGRAAMKKSGGIPWQTAPEPVCSQPSSPKA"
BASE COUNT          766 a         1187 c         1187 g          783 t
ORIGIN      
        1 ccggagttac actgccgcca ggggacgcgt gcaggttgcc atcatggggg aagaataagg
       61 ggggccgttg atgccgcgaa gtactgttgc cgctttatat acatcgccgg agagggtgct
      121 cgacgatatt ggacgcctgg cagagctggc cggaatgcgc gaggcgctgg atgcgcgttc
      181 gaccactatc ctgaaagaca atatctcctg gcactttccg tttcccggcg caaataccac
      241 gccctggcag cttgaaggca cgatcctggc gctgcgcgag cgcggattca aagacctttc
      301 ctgcgtgcag aacaagacgg tggtcaccaa cgcgttcaaa ggcgaggacc tgaaccacta
      361 cgtgccggtt ttccggcgtt acgaaatccc ggtgttctac aacttccacg acgcagacat
      421 gaagtggatc gagtacaagc ccaaagggaa gatgctggtg ctcgacaaaa tcttccgcga
      481 agggatctac gttccggatt atttcttcgg gaaaaatatc gttcatctgc ctacggtgaa
      541 gtgccacatc tacaccacca caacgggctc gatgaagaat gcgttcggcg ggcttctgaa
      601 cacgcatcgc cattacaccc actcctggat tcacgaaacg ctggtggacc tgttgacgat
      661 tcaaaaagag atccacgccg gacttttcgc cataatggac ggcaccacgg ccggcgacgg
      721 tccggggccg cgcaccatgc gtccgaagat caaaaacctg atgctggcca gcgcggacca
      781 ggtcgccatt gatgccgtgg cggcgaagat catgggattc gatccgatga gcctgcctta
      841 tatccacctg gcgcacgagc gcgggttggg cgtgggcaag cccgaggaga ttcacatcgc
      901 cggcgacgac atccggaatg agagctgggg cttcagcgtc ggtgacaaca tggcgagccg
      961 catcggcgat atctgctggt tcggacccct gaaacgcatt cagaagttac tgtttcattc
     1021 gccgctggta cacatgttcg tcttcgggtc ggaggcctac catgactggt tccgctggcc
     1081 ggtcaaagac aggcgggttt acgagcagtg gctccggacg accggatggg gccgcctgtt
     1141 cggcgaatac gcgcgatacg gtacgcgcac atccgccgct tcggcggcgg actaagtaca
     1201 tggttctcgc tttacgtgga caccgctccc tgacggtcgc ggctcagtat gggcctcatt
     1261 ccgagccgcg accgtcaggg agcggtcgtt cgcctgggtt gaaatgatgc gtgtcgccat
     1321 agacgccacc tgctggcaca ataaccgggg ctatggacgg cacgcccgcg cgctggtttc
     1381 gacgcttgtc cgccagtatc cggagaatga ataccggctg tttctggact ctgcgggcaa
     1441 cacgcccgat cttgccgggc acgcaaaact ggaactggtg gcggcggaca caccggcctc
     1501 ggcggcggct tcgtccaacg gccaccggtc gctgtccgat atgtggagaa tgagccgggc
     1561 tctgtcgaaa gcccgtgtgg acgtgttgct ctatccgacc atctatacgt atgttccggc
     1621 actgacgcgc gcccggaaac tcgtcatgat tcatgacgtg attgcggaga cctatccgaa
     1681 tctgacactg ccccggcgga cggcacgcct gttctggaag gccaaagtcg ccttggggcg
     1741 tatgcaggcg gacgcactgg tgaccgtctc cgagttctcg cgcgagaaaa tcatcgaaca
     1801 tttccgtgtt gctccggaac gcgtctttgt cgtcagtgaa gccgcggacc cggttttccg
     1861 catcctggag cagccggcca tgcccgcatc cctgcgggcg ctcggcatcg acgccggccg
     1921 ccgcatcgtt gtttacgtcg gcggcttcag cccgcacaaa aacctggagg agctgatgga
     1981 cgcgttctcg cacatcgctt ctctcggaca gttcgacgat tgcctgctgg tgctggtggg
     2041 agagacgcgg aaagaagtgt ttcacagttg ccttccagcc cttcgcgaga aagtggatca
     2101 agccggcctg cgcgatcacg tcgtgtttcc gggatacgta tccgacgagg atctgacgga
     2161 gttgttgaac ctttccgcgg tgctggtgct tccctctttc atggaaggct tcggactgcc
     2221 cgcggtagag gcggcggcgt gcgggtgccc ggtgatcgcc accaaggcca gcccgctgcc
     2281 gcaactgctg gaggggggag gaatctacat cgagccggga agtggtcaac tggagcaggc
     2341 gctggaggcg gtactggctt cgaaagagat gcgggaacgt atgcggcggg ccggcgccgc
     2401 ggcggcgcaa cggctcacct ggaatgccgc agcagagcaa ctgatgcagg tgatgcaaag
     2461 gttcgagtaa ccactgccat gaactttctt cacctgacca cgttttatcc gccttacagc
     2521 ttcggtggcg atgccatgta catctaccgc ctttcgcatg ccctggcaga ggcgggccat
     2581 cacgtggatg tcattcattg cgtcgattcc taccaccttc tgcatccggc gaaaccgccg
     2641 atccaatttg cggaacaccc gaacgtgacg cgtcacgagc tgcgcagcgg ccatggctgg
     2701 ctgtctccgt ttctgactca gcagaccggc cggccgtacc tcaaaacaga cacgatccgc
     2761 aaagtgcttt cctcgcgacg ccacgacgtg atccactttc acaacatgtc gctgctgggg
     2821 ccgcagacgc ttgccatcga gccgcccaat cccgcagcgg tgaagatcta cacgacgcac
     2881 gaacactggc tggtctgtcc catgcacgtg ctatggaagt tcaacagcag ggcatgcgaa
     2941 aagcccgagt gcctgaaatg cacgctcctc ggaaaacggc cgccgcagtg gtggcgctat
     3001 accggcttgc tcgaacgcgc ggcgcggcgc gtggatcagt tcgtctcgcc cagccgcttc
     3061 acggcgaaga tgcatgccag ccggggcttt tcgcaaccgg tggagcacct gccgtatttc
     3121 atcgaccgcg ccgatgacga ttggcgcacc cccagcccgc gcccgcagga acggccctac
     3181 ttcctgttcg tcggccgtct ggaaattatc aaagggctcc agacgttgat cccgctttgg
     3241 aagaacgtgg agtttgatct tctggtggcc gggaccggca actatgaagt ggagctgcgc
     3301 gccatggcgg catcgaatcc gcggattcgc tttctgggcc ccatgtcaca acggcaattg
     3361 ggcgcgctct atcatcatgc gctcgcctgc atccttcctt cgatcactta cgagaccttc
     3421 gggatgatca gcatcgaagc cttcgcgcgc aagacgccgg tcatcgtgcg cgacctcggc
     3481 gcgctgcccg aagccgtcga ggattccggc ggcggcttcg tctatcgaag cgaggaagag
     3541 ctgctcgaag ccatccggca gatcggcgct tcgcccggcc tgcggcgcga gcttggggag
     3601 aaaggctacc gcggttttct cgagtggtgg tgccgggagg cgcacatgcg cttctacttc
     3661 gattacctcg gccgcgcggc aatgaaaaaa tccggaggca tcccctggca gaccgcaccc
     3721 gaaccggtct gctcccagcc gtccagtcca aaagcttagg aatctggtct ctttttttga
     3781 aaagaggccg gcttcccaac gctaaaactc tccatttttc gggctccagt tgggccctgt
     3841 acgtgccttc ctgcctgtaa tacccatgat ttgcaatctg gaggcatggt tgacgaggtt
     3901 cgcatggcaa catttctagt aac
//
LOCUS       DASTMK010000413         9103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_629094, whole genome shotgun sequence.
ACCESSION   DASTMK010000413 DASTMK010000000
VERSION     DASTMK010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629094"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1597
                     /locus_tag="VFQ79_17740"
     CDS             209..1597
                     /locus_tag="VFQ79_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0141571.1"
                     /translation="MPEKPAALVRQLGIVSAAALVISNMVGTGIFTASGFLAADLGSP
                     SLILTIWMVGALFALAGALCYSELGINFPSSGGEYVYLTRAYGPTWGFMTGWISFFAG
                     FSAPIAAASLAFSDYVGYFIPGAQQANAQFTIGSGAWSLKVGGAQLVASALIIVFTLL
                     NIFGIGRVAKIQNVLTAIKVLVIIGLVGLGFLIGTGSWSHFSMDAVRTSTRSLPAQFA
                     ISLFWIYVSYSGWNAATYIAEELKRPERTLPVALTVGTLLVAAMFIAMNVVFIYATPL
                     ETMKGVVAVGSLAASHLFGPGIAGAFSALMALSLMSTVNAMVTVGPRVYYAMAKNGAF
                     LPAAAKVDSRWHTPVVAILCQGACAVVMTLTPFRDLMIYIGFLLNFFAVMSVASLFIF
                     RRRPGWQKLRVVSFAFPLIPLLFVLVGAWMTIFGLTLQPKIAVAAIVTIATGALVYHF
                     RLRSSARAGSHV"
     gene            1622..2542
                     /gene="folD"
                     /locus_tag="VFQ79_17745"
     CDS             1622..2542
                     /gene="folD"
                     /locus_tag="VFQ79_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU0141572.1"
                     /translation="MAKILDGKWVRDEILKDCVSRIERLSAAGRPPGLAVVLVGNDPA
                     SEIYVRNKVKTSGDLGIYSEKLTPSASVTTEELLEIVEGLNARADIDGILVQMPLPAQ
                     VDSARVLLAVDPEKDVDGFHPLNVGNLVTNRPGPRPCTPSGIMEMLRRYDIPIKGKRA
                     VVVGRSDIVGKPMAMMLLHAHATVTICHSRTSDLQGECRRADILVGAIGRPALLTADF
                     IKPGATVIDVGMNRIADAATARSVLAGATEKLAEFDRKGTVLTGDVHPGDVARTAGAY
                     TPVPGGVGPLTIAMLMVNTIDAAEQRMGIG"
     gene            2547..3155
                     /gene="coaE"
                     /locus_tag="VFQ79_17750"
     CDS             2547..3155
                     /gene="coaE"
                     /locus_tag="VFQ79_17750"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU0141573.1"
                     /translation="MLRVGLTGGLASGKTNVGQALESMGCYLIQADRLGREVLEPGGP
                     AYASVLKEFGTGILEEDGSINRRALAAQVFANPDRLAVLNSLVHPHVIELEGKLLAEY
                     ERREPHGIGIVEAAILIETGSFKRFDKLILVMCSQEQQLERAMKRDGLSYEEVQARLE
                     RQMPLEEKRQYADYVIDTSGSKEETLAQTREVYNSLRSLIQK"
     gene            3152..4306
                     /locus_tag="VFQ79_17755"
     CDS             3152..4306
                     /locus_tag="VFQ79_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0141574.1"
                     /translation="MRKYRFLILTALIVGVFVYLTSVSQWPRRWGTNVSDAGGNLWSE
                     PVVAHTASLSADEINNIEIYKMAHAATVNITSTVYRRGWFMQIVPDPSTGSGFVIRDD
                     GLILTNYHVVSGRAPEIQVTMPDKSRYKASILARDYPNDLALIRIGPRKKIDFLRLGD
                     SDHLQVGQKVLAIGNPFGLEGTLTTGIVSSIGRTIADQSGRELEGMIQTDAAINPGNS
                     GGPLLDSFGNVVGINTAIYGPGGNIGIGFAMPISRAKSMIERYQAGGSFARPRLGVST
                     VYVAGDLAQMLNLPAEGGLLVQEVGPGSPAASAGIRGPRQVVIVGNLEIGVGGDLITE
                     VDGRPIDTDDAVRRALDKKHAGDKLELTVFRDGQSRKVVVTLGAASEENF"
     gene            4364..5089
                     /locus_tag="VFQ79_17760"
     CDS             4364..5089
                     /locus_tag="VFQ79_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU0141575.1"
                     /translation="MGPRVSIFVTCVVDQLFPRVGLAMADVLERAGCLVEFPERQTCC
                     GQPAFNSGYHEDARRVARHFLDVFKDSEYIVVPSGSCTSMIAHHYADLFRDSPEWLDR
                     AKQIAPRVWEFSTFLVDVLQIADVGASLETVATYHDSCHALRELHVKEAPRKLLRHVR
                     GLELREMDAAEECCGFGGTFAVKFPGVSGGMARTKIDSIARTNADVVVSVDSSCLMQI
                     QGALSRAGLPTKTMHLAEVLAAR"
     gene            5094..6518
                     /locus_tag="VFQ79_17765"
     CDS             5094..6518
                     /locus_tag="VFQ79_17765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEU0141576.1"
                     /translation="MTSLPEANIDRTLADANLQLAIYASTGRLMDKRKTAVSAELLPD
                     YQELRTQANAVKKHALDNLDFYLEQFDTNVQNVGGHVVYCRDGSEVADFVLKLAKDRG
                     AGLLVKSKSMTTEEIDLNERLEHHGLHAVETDLGEYILQLAEERPYHIVAPALHKTRY
                     DVADIFTSKLGVEREIVPENQTRIAREVLREKFLAADIGLTGANFLVADSGAVVLVEN
                     EGNARLTSSAPKIHIAVAGIEKIIPRAQDLAVFLKLLGRSATGQHLTVYTSFLSGPRR
                     NGEIDGPDEFYVVLLDNGRTRLLADPEKRQSLYCIRCGACLNICPVYRKIGGHNYPWV
                     YSGPIGAIITPQYQGIAKNPWLPYASSLCGACAEVCPVKIDIPKLLLDLRSEVTATGP
                     KDQKTRIERLSFRIWSWLMTKPKLFEMAGMAASTALANGSGWLRNVPAPLNAGPLRAW
                     ISQRDLPVPPSKSFRQLWRSRPNK"
     gene            6520..7152
                     /locus_tag="VFQ79_17770"
     CDS             6520..7152
                     /locus_tag="VFQ79_17770"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEU0141577.1"
                     /translation="MSRDDILQQVRSALGRKPGEAPPDAPPARICISQPDTDERIRSF
                     VCSLEGLAGKAFVAGSGEEARQYAADLLENGTAVASNAPFLEQLGIRTLPGVQSGFYG
                     RDELRTACASAGLGITGADYALADTGTLVLISSPEEARLISLLPPVHLAFVARENILS
                     GLDELFAILPKPAARTSSMVLITGPSRTADIEQILIRGVHGPGHVHVVIV"
     gene            7195..7791
                     /locus_tag="VFQ79_17775"
     CDS             7195..7791
                     /locus_tag="VFQ79_17775"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0141578.1"
                     /translation="MRHITPILLAAAFFAGQPLAHAQVAEFAVIGGASRLSNAGIGSF
                     DFENPREDDVELTDGWRLGFRMTLNNWRFLGHEFGYAYNRTKLKVGSDEAGMAIHQGF
                     YNFLAYATPEGTFIRPFATGGVHFANYTPPGISVTQGGGDTKFGVNYGGGLKIRVSHN
                     LMLRFDVRDYLTGKPFDLPGQRGKLHQLEIAVGLGIAL"
     gene            complement(7811..8341)
                     /locus_tag="VFQ79_17780"
     CDS             complement(7811..8341)
                     /locus_tag="VFQ79_17780"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HEU0141579.1"
                     /translation="MKAIHLMAVDTAPDLGVLDRLSASLAGVFGISCRVLDTVIDPAF
                     ALDPARNQYHSTAILQAMQPLVGKPGTHLLAVTPVDLYVPILTFVFGEAQLSGPCALV
                     STHRLREEFYGLPPREPLFNDRLVKEAVHELGHTFGLRHCPDWRCAMASTHSVERLDL
                     KAAWFCERCRQTVRQP"
     gene            complement(8350..>9103)
                     /locus_tag="VFQ79_17785"
     CDS             complement(8350..>9103)
                     /locus_tag="VFQ79_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0141580.1"
                     /translation="MPMVVIHCGALTETLLESELFGHERGAFTGAQYRKKGKFEVAEG
                     GTIFLDEIGDISLKTQTDLLRVLQEKEIVRVGGNETLAVDFRCVAATNKPLEMMVKEG
                     TFRPDLFYRLNVFSIHLPPLRDRKEDIPILVSHFLEKYAAAMNRPVPDIGGEGLDLLI
                     NYNWPGNIRELENAIERAMVINRGQEIKASDFPFQLNAPEPVQGKTLEDIERIHIQRV
                     LEETNWNLSRTARILAIDRTTLYNKLRRYGMR"
BASE COUNT         1819 a         2671 c         2728 g         1885 t
ORIGIN      
        1 caggagaacg atcgatgccg ttgcaaccac caggttcccc atacttcaat ggtaggcggt
       61 tcaagtgtca agaaccagcg gatctgcgtg aacaccagaa aaaagcacgt agttctggca
      121 gattgttcag cggcttcgcc acatgcgggc gatcgaccgg cgtacaatgt gcaaggtggg
      181 accagcaaaa accggccgta cacgccctat gccagagaag cccgcagcgc ttgtccgcca
      241 gctcggaatc gtcagtgccg ccgccttggt gatctcgaac atggtcggga ccggcatctt
      301 cacagccagt ggttttctag ccgcagatct cggctctcca tcgctcatcc tcaccatctg
      361 gatggtgggc gcactattcg ctttggccgg tgcgctctgc tattcggaat tggggattaa
      421 ttttccaagc tccggcggcg aatacgtgta tcttacccgt gcctacggcc cgacatgggg
      481 tttcatgacc ggctggatct cgttcttcgc cggattctca gcgccgattg cagccgcctc
      541 gttggccttc tccgattacg taggatattt catcccggga gcgcagcagg cgaacgcgca
      601 gttcacgatc ggctccggtg cctggtcgct gaaggttgga ggagcacagc tcgtcgcctc
      661 cgcactaatc atcgtcttca cgctgctgaa tatcttcggg atcggacgtg tcgccaaaat
      721 tcagaatgtc ctcaccgcga taaaggtgct ggtcattatc ggactcgtgg gtctcgggtt
      781 tctcatcggc accggaagct ggtcgcactt ctccatggat gcggtgcgta catcaacacg
      841 ttccctgccg gcgcaattcg ctatcagcct gttctggatt tacgtctcct acagcgggtg
      901 gaacgcggcc acctacatcg cagaggaact gaaacggccc gagcggactt tgcccgtcgc
      961 gctcactgta ggaaccctgc tggtcgcggc gatgttcatc gcgatgaacg tggtatttat
     1021 ctacgcaact cctctcgaaa cgatgaaagg cgtggtagcc gtcggctccc tggcagcttc
     1081 ccatttattc ggaccgggta tcgcgggcgc attcagcgcg ctgatggcgc tgtcgttgat
     1141 gtccactgta aacgccatgg ttacggtagg cccccgcgta tactacgcca tggcgaagaa
     1201 cggagcgttt ctgccggccg ccgcgaaagt ggacagcagg tggcacactc ccgtggtcgc
     1261 gattctatgc cagggtgcct gtgcggtggt gatgaccctg acgccgttcc gcgaccttat
     1321 gatttatatc ggatttctgc tgaacttctt cgcggtgatg tctgtggcct ccctgttcat
     1381 tttccgcaga aggccgggat ggcagaaact ccgcgtagtg agctttgcgt ttccgctcat
     1441 tccgctgttg tttgtcctgg taggcgcctg gatgacgatc ttcggattga cgctccagcc
     1501 gaaaatcgcc gtcgcagcta ttgtcacgat cgcgacgggc gcgctggtgt atcacttccg
     1561 tcttcgctcg tccgcgcgcg cgggttcgca cgtctaaata gtttcaagtt tcgctggcaa
     1621 aatggcaaaa atactcgatg ggaagtgggt gcgtgacgaa atactgaagg actgtgtctc
     1681 ccggatagag cggctgtccg cggccggaag acctccaggg ttggctgtcg tgctcgttgg
     1741 aaacgacccg gcgtccgaaa tctacgtccg caataaagtg aagacctcgg gcgacctcgg
     1801 catttatagt gagaagctga cgccgtccgc ctcggtcaca accgaagagt tactggagat
     1861 cgtggagggc ctcaatgccc gagccgacat cgacgggatt ctggtgcaga tgccgctgcc
     1921 cgcgcaggtc gattccgcac gggtgctgct ggctgtcgat ccggagaagg acgtcgatgg
     1981 ctttcatccg ctgaacgtgg gcaatctcgt cacgaaccgt ccgggaccgc gcccgtgtac
     2041 accctccggc atcatggaaa tgctgcgccg ctacgatatc cccatcaagg ggaaacgtgc
     2101 cgtcgtcgtg ggccggagcg atatcgtcgg caagccaatg gcgatgatgc ttctgcatgc
     2161 gcatgccacc gtgacgatct gccattctcg tacgtctgat cttcaggggg agtgtcgccg
     2221 ggccgacatc ctggtgggcg ccatcggacg cccggcactg ctcaccgccg atttcatcaa
     2281 gcccggagcg accgtgatcg atgtcgggat gaaccggatc gccgatgccg caaccgcgcg
     2341 gtcggtgctg gcgggggcta cggaaaagct ggcggagttc gatcgcaaag gtacggtgct
     2401 gaccggcgac gttcatcccg gcgatgtagc gcgtacggca ggcgcctaca cgcccgtgcc
     2461 tggcggcgtg gggcccctga cgattgcaat gctgatggtg aatacgatcg atgcggcgga
     2521 acagcgcatg gggatcgggt aatcggatgc tgcgggtcgg cttgaccgga ggtttggcga
     2581 gcgggaagac caatgtcggc caggcgctcg agtcgatggg atgctacctg attcaggccg
     2641 atcgactggg acgggaagtt ctggagcccg gcggacctgc ctatgcctcc gttctgaagg
     2701 aatttggcac cggtatcctg gaggaagacg ggagcattaa ccggagagcg ctggcggcac
     2761 aggttttcgc caacccggac cggctggcgg tgctcaacag cctggttcac cctcacgtca
     2821 ttgaactcga gggcaaactg ttggcagagt acgaacgccg ggagccgcac ggaatcggga
     2881 tcgtcgaagc ggcaattctg attgagaccg gcagctttaa gaggttcgac aagctgattc
     2941 ttgtcatgtg cagccaagag caacaactgg agcgtgcaat gaagcgcgat ggccttagct
     3001 atgaggaagt gcaggcgcgt ctcgagcggc aaatgccgct cgaagagaaa cggcaatacg
     3061 ccgactacgt gatcgacacc tctggcagca aagaggagac gctggcgcaa acacgcgagg
     3121 tttataactc gctccggagc ctgattcaaa aatgaggaag tatcgttttc tgattttgac
     3181 agccctgatc gtaggggtct tcgtatacct gacctcggtt tctcagtggc ctcgccgctg
     3241 ggggacaaac gtgagtgatg cgggcggaaa cctttggtca gagcccgtgg tagcccatac
     3301 ggcgagcctc tccgcggacg aaatcaacaa catcgagatt tacaaaatgg cgcacgccgc
     3361 aacggtgaac attacaagca cggtttaccg ccgcgggtgg ttcatgcaga ttgtacccga
     3421 ccccagcacc ggttccggat tcgttatccg cgatgacggt ctgatcctta ccaattacca
     3481 tgtcgtatcc ggccgtgcac cggagattca ggtgaccatg ccggacaaga gccggtacaa
     3541 ggcgtccatt ctggcgcgcg attatccgaa cgatctcgct ctcatccgga tcgggccacg
     3601 gaagaagatc gacttccttc ggctcggcga ctccgatcat cttcaggtgg gacagaaagt
     3661 gcttgcgatc ggcaacccct ttggcctgga aggcacactc acaacgggca tcgtcagttc
     3721 catcgggcgc accatcgcag accagagcgg ccgtgaactg gaaggcatga ttcaaaccga
     3781 cgcggcgatc aatcccggca atagcggcgg cccgctgctc gattcttttg gcaatgtggt
     3841 tggaattaat actgccatat acggacccgg aggaaatatc ggcattggct ttgcaatgcc
     3901 catcagccgc gccaaatcga tgattgaacg ctaccaggcc ggcggcagtt ttgcccgccc
     3961 gcggctgggc gttagtacgg tttacgtcgc cggggatctt gctcagatgc tgaaccttcc
     4021 tgctgagggc ggcctgctgg tgcaggaagt ggggccaggt tcgcccgccg cctccgccgg
     4081 catacgcggt ccaaggcaag tggtcatcgt tgggaacctc gagattggag taggcggaga
     4141 tctcatcacg gaagtcgatg gccgtcctat cgacaccgat gacgccgtgc gccgcgcgct
     4201 cgataaaaaa cacgcgggcg acaagttgga gctgacggtc tttcgcgatg gccagagccg
     4261 caaagtcgtg gtaacgctcg gcgcggcgtc agaagagaat ttttagcgcc ggcgcacggc
     4321 gcccacccgg cagccgtaca gccggtgtta caatctcagc ttcatgggtc cacgggtatc
     4381 gatctttgtc acttgcgtcg tggaccagct tttcccgcgc gtcggactgg cgatggcgga
     4441 cgtgttggag cgcgcgggat gtctcgtcga gtttccggag cggcagacct gctgcggcca
     4501 gccggcgttc aacagcggat atcacgagga cgcgcgcagg gttgcgcggc actttctgga
     4561 tgttttcaag gacagcgaat acatcgttgt tccttccggc tcctgcacgt ccatgattgc
     4621 gcatcattac gcggatctct tccgcgatag cccggaatgg ctggaccgtg cgaaacaaat
     4681 cgctccgcga gtgtgggagt tttccacttt cctcgtggat gtcctccaaa tcgcggacgt
     4741 cggcgcaagc ctcgaaaccg tggcgacgta tcatgacagt tgccatgcgc ttcgggagct
     4801 gcacgtcaag gaagcaccac ggaaactgtt gcgccacgtg cgcggcctgg aattgcgaga
     4861 gatggatgcg gcggaggaat gctgcgggtt cggcggtacg ttcgccgtga aatttcccgg
     4921 cgtgtcgggc ggcatggcac ggacgaaaat cgattccatc gcgcgtacga acgccgatgt
     4981 ggttgtatcg gttgattcca gttgcctgat gcagattcaa ggcgctctgt cccgggcggg
     5041 gctgccaacg aagaccatgc atctggccga ggtgctcgcc gcgcggtaga tccatgactt
     5101 cccttcccga agccaatatc gaccgaaccc tggcggatgc gaatctccag cttgccattt
     5161 acgcttccac cggacggctg atggacaaac gcaagacggc ggtatcggcg gagctgctgc
     5221 ccgattacca ggaacttcgc acgcaggcga acgccgtcaa gaagcatgct ctcgacaatc
     5281 tggatttcta cctcgagcaa tttgatacga atgttcagaa cgtgggtggt catgtcgtct
     5341 actgccgtga cggcagcgag gtagccgatt tcgtgctgaa gctcgccaaa gacagaggtg
     5401 ccgggttgct ggtcaagtcg aaatccatga ctaccgaaga gatcgatttg aacgagcggc
     5461 tggagcatca cgggctccat gcggttgaaa ccgatctcgg tgagtacatc cttcaattgg
     5521 cagaggagcg gccctatcat atcgtcgctc ccgccctgca caagacccgc tacgacgtgg
     5581 ccgatatctt cacgtcgaag ctgggcgtgg agcgggaaat cgtgcccgag aatcagactc
     5641 gaatcgcccg cgaggtgctg agagagaaat ttcttgcggc cgatatcggt ctcacgggcg
     5701 ccaatttcct ggtggcggat tccggtgccg tcgttctggt cgaaaacgaa gggaacgccc
     5761 gcctgaccag ctccgcgcca aagatccaca tcgccgtggc gggaattgag aagatcatcc
     5821 cccgcgcgca ggatctcgcc gtattcctca agctgctggg ccgcagcgcc accgggcagc
     5881 accttacggt ctacacttcg tttctctccg ggccgcggcg caacggcgag atcgacgggc
     5941 cggacgaatt ctacgttgtg ctcctggaca acggccgcac gcgtctgctc gccgatcctg
     6001 aaaaacgcca atcgctctat tgcatccgct gcggtgcgtg tctgaacatc tgccccgtgt
     6061 accgcaagat cggcggccac aattatccat gggtctattc cggcccgatc ggcgccatca
     6121 tcacaccgca ataccaggga atcgcgaaaa atccctggct gccctacgct tcgagccttt
     6181 gcggcgcgtg cgcggaggtc tgtccagtta aaatcgacat acccaagctg ctgctcgatc
     6241 tccggtcgga agtcaccgcg acaggcccga aagatcaaaa gaccagaatc gagcgcctgt
     6301 cttttcgaat ctggtcctgg ctgatgacga agccaaagct ttttgagatg gccggcatgg
     6361 ccgcttccac cgcactcgct aacggatccg ggtggctgcg gaacgtaccc gctccgctga
     6421 atgccgggcc tctgcgggca tggatcagcc agcgtgatct tcccgttccg ccgtcgaaga
     6481 gcttccggca actctggcgc tcaaggccca acaagtaaga tgtcacgcga cgatattctt
     6541 cagcaggttc gatccgccct cggccgaaag cccggcgaag ccccgccgga tgcgccgccc
     6601 gcaagaattt gcatctcaca gccggacacg gacgaacgga ttcgttcttt cgtatgctcg
     6661 ctcgagggtc tggccgggaa agcctttgta gcgggttcgg gggaagaagc ccgccagtat
     6721 gccgcggacc ttctcgaaaa cgggacggcg gtggcgtcaa atgctccatt cctcgaacag
     6781 ttgggcatcc gaaccctgcc gggcgtgcaa tcgggcttct atggccggga tgaattgcga
     6841 acagcctgcg ccagtgccgg cctgggcatc acgggcgcgg attatgctct ggccgatacc
     6901 gggacactgg ttttgatctc ttcgccggaa gaagcacgtc tcatctcgct gttgcccccg
     6961 gtgcatcttg ccttcgtcgc gcgggaaaat atcctttcgg gattggacga gttattcgct
     7021 attttgccca agccggcggc aagaaccagt tccatggtcc tgattaccgg gccgagccgc
     7081 acggcggata tcgaacagat cctgatccgg ggcgtccacg gccccggtca cgttcatgtg
     7141 gtaatagtct agaagaaacg gaacttgcga tcaccgcatc caaggtttaa acgaatgaga
     7201 cacataacac ctatcctgct cgctgccgcc ttttttgccg gtcaacctct ggcgcatgcc
     7261 caagtcgccg aatttgccgt catcggcggc gcctcccgcc tgagtaacgc cggtatcggc
     7321 tctttcgact tcgagaaccc gcgcgaggac gatgtcgaac tgacggacgg atggcgcctg
     7381 ggctttcgaa tgaccctgaa caactggcga ttcctgggcc atgagttcgg ctacgcttac
     7441 aaccggacca agctgaaggt tgggtcggat gaggcgggta tggccatcca tcagggattt
     7501 tacaatttcc tcgcctatgc tactcccgag ggtaccttta tacggccgtt cgctaccgga
     7561 ggtgttcact ttgccaatta cacccctccc ggcatatcgg tcacccaagg cggcggcgat
     7621 accaaattcg gggtgaacta cggcggtggt ttaaaaatcc gggtgtctca caatctcatg
     7681 cttcgattcg atgtgcgtga ttaccttaca ggaaaaccat tcgatctgcc cggccagagg
     7741 ggcaaactgc atcaactcga gatagcggtc ggcttgggga tcgcgctgta gacacgaacg
     7801 cgtcatttcc ttagggctgc cggaccgtct gcctgcatcg ctcgcagaac catgcggcct
     7861 tcaggtccag gcgctcgaca gagtgcgtgg atgccatggc gcatctccaa tccgggcaat
     7921 gccgcaaacc aaatgtgtgg ccgagctcgt gaaccgcttc ctttaccagg cgatcgttaa
     7981 acagcggttc gcgcggcgga agcccgtaga actcttcccg cagccggtgc gtggagacca
     8041 gcgcgcaggg tcccgaaagc tgtgcttcgc cgaagacgaa cgtaaggatc ggcacataca
     8101 gatcgaccgg agtgacagcg agcaggtgag tcccaggttt cccgaccagc ggctgcatgg
     8161 cctggagaat cgccgtcgaa tgatattggt tccgcgccgg gtcgagcgca aaggccgggt
     8221 cgatcaccgt gtccagaacc cggcaggaaa tcccgaatac accggcaagc gaagccgaga
     8281 gccggtcgag aacgcccagg tccggcgccg tgtccaccgc catcaggtgg atggccttca
     8341 tggatcactt cacctcatcc cgtagcgtct cagcttgttg tagagtgtcg tacgatcgat
     8401 ggccagtatc ctggcggtac gggacaggtt ccagtttgtc tcttccagga cacgctggat
     8461 gtgtatccgt tcgatgtctt cgagcgtctt gccctgtacc ggctcgggtg cattgagctg
     8521 aaacggaaaa tcggacgctt tgatctcctg gccacgattg atcaccatcg cccgttcgat
     8581 ggcgttctcg agttcccgga tgttgccggg ccagttgtag ttgatcagca gatcgagtcc
     8641 ttcaccgccg atgtcgggca ccgggcgatt catcgcagcc gcatacttct cgaggaaatg
     8701 gctcaccaat atgggaatat cctctttgcg atcgcgcagc ggcggaagat gaatggaaaa
     8761 aacattcagc cggtaaaaca agtccggccg gaaggtgcct tccttcacca tcatctccag
     8821 cggtttgttg gtggcggcca cgcagcggaa gtccactgcc agcgtttcgt tgccgccgac
     8881 acgcacgatc tctttctcct gcaagacacg gagcaggtcg gtttgtgtct tcaggctgat
     8941 gtcgccgatc tcatcgagaa aaatggtccc accctcggcc acttcgaact tgcccttctt
     9001 ccggtactgc gcgccggtaa aggcaccgcg ctcatgtccg aacagctcgc tttcgagcag
     9061 cgtctccgtg agggcgccgc agtgaatcac taccatcggc ata
//
LOCUS       DASTMK010000414        10258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_372886, whole genome shotgun sequence.
ACCESSION   DASTMK010000414 DASTMK010000000
VERSION     DASTMK010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372886"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..879
                     /locus_tag="VFQ79_17790"
     CDS             112..879
                     /locus_tag="VFQ79_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEU0141581.1"
                     /translation="MRFEKDLQAARDAAIRAGELALIHRSKGLAPEDKADLSPVTIAD
                     RESERLLVTALTGAFPDDGILGEEGANHTGSSGRHWIIDPIDGTREFLRGSSAWCVLV
                     GLEAEGEVELGVAHFPAMKTTFWAVRGEGAFHDGERISVSSISSADRAVLCLNGFNNL
                     GKHSFQDRLLPFLSRYWSVRSLGGCLDAVMVAQGQADAWIEPSAQPWDLAALKVITEE
                     AGARFFNFDGGSSIHAGNCVTCVPALEAELRAFVRNG"
     gene            883..1881
                     /locus_tag="VFQ79_17795"
     CDS             883..1881
                     /locus_tag="VFQ79_17795"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU0141582.1"
                     /translation="MRAVVLLLAGMPFAGFAQSPATLYAEARALADTNTPEAIRRLED
                     IVQRWPEFVEAHLSLGRIYLTPGFRNREKAIEHLQRAMDLQPDSLEPYRLLTALPPSS
                     FLSQSARRMRELLERSTDPDDLAAFPVLWAIEFRTTPPSGHSAVRDRLKEDVERLKTA
                     GSRPSPAVLYALSEARRILGTAETPGMGWRQMLKPLPAFRLPDLAGRVWTPDDLKGKV
                     TLINVWATWCGPCRVELPHIQRLHEKVKDRTDLLVLTLNVDGHPGRVDSYLKEGGYTF
                     PVIPAGDYVLEATPVFVVPQTWIVDGSGMIREQSVGFAGADEGWVDEILRRMKQWK"
     gene            1938..2450
                     /gene="coaD"
                     /locus_tag="VFQ79_17800"
     CDS             1938..2450
                     /gene="coaD"
                     /locus_tag="VFQ79_17800"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU0141583.1"
                     /translation="MHPLATKPPCVIAIYPGSFDPITNGHLDLIERSARLVDKLIISI
                     LRNENKQPLFSVQERMDMLRDVVGPYPNVEVDSFDGLLVDYAAARSATVILRGIRAIS
                     DYEYELQMALMNRRLRPQIETVFLMSGEAYSFISSRLVKEVFRLGGDISGLVPPPVEQ
                     RLKNRFLGEK"
     gene            2457..3641
                     /locus_tag="VFQ79_17805"
     CDS             2457..3641
                     /locus_tag="VFQ79_17805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0141584.1"
                     /translation="MQAALELAERVSLISVSSTMKVAADADRLRREGVDVVDFGAGEP
                     DFPTPENIKQAAIQAIAEDFTKYTAAGGTPELKQAICERHRHDYGTAYSPAECIVSVG
                     GKHVIFNLMQVLLNAGDEVVIPVPYWVTYKDVVNYAGGKCVFVETDEAAGFALTAAML
                     EPYITDKTKLIVINSPSNPSGAVLDREEFERIYHLASQRGVWLLTDECYCRFLYEGDP
                     FSIAAMPGAKDHVLVAGSLSKTYAMTGWRIGFGLAPASIIGAINKLQSHSTSNPTSIA
                     QKAAVEALRGPQDSVGVMLDEYRTRRDFVLKRLRQIPGVTIQEPKGAFYAYPNIGAAL
                     GKNGIRTPMQFAEKLLGEAHVAVVPGEAFGTDRHVRISYAASLDELSRGLDRMHEFIA
                     KI"
     gene            3642..4451
                     /locus_tag="VFQ79_17810"
     CDS             3642..4451
                     /locus_tag="VFQ79_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEU0141585.1"
                     /translation="MPVQKLTPSFREKVWGATDLTPWFPVQQTRIGEVWFESERPLPL
                     LVKFLFTSERLSVQVHPGDDYARKHENSPGKTEMWHVLRTEGDARIALGFHERLSRET
                     LRRASETGEIEHLLNWVPVQAGDSIFAPAGTVHAIGPGLALCEIQQVSDITYRLHDYG
                     RPRELHLNKAMDVANTEPHPGKAVPRPLPDGSLLLVCCEYFCTEKAVVSTEVPWQPRG
                     SNPEILVVLEGTGYLENKVFGPGEAWLVDEPVVLQPQGTAVLLRTYVPSAA"
     gene            complement(4464..4631)
                     /locus_tag="VFQ79_17815"
     CDS             complement(4464..4631)
                     /locus_tag="VFQ79_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141586.1"
                     /translation="MYQEAPDPAAPQQEDLYCPKCAKPVQEPLVCGDCLAVICRDCGT
                     PLERTDDLGIG"
     gene            4662..5897
                     /locus_tag="VFQ79_17820"
     CDS             4662..5897
                     /locus_tag="VFQ79_17820"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0141587.1"
                     /translation="MPLSRLLLSSLIVATLSLAGCGSRDEGPKPIGHAFVAQPDLKLR
                     AELDAKAPATATVQNGERVEILRTFRRFVKVRTKTGAEGWVHERQLMSSREIDKLDRA
                     SERLRELPSQGSATVYATLNVHIEPHRQSPTFYQIREKTHVTVLGHQLVPRGPYKPEP
                     LLPPPPPKPKAVKKPSRPSQAQTPPIVTPDPPGPPEDWLEISKSPLPPEPEPEPEQTP
                     IPGEDWSLVRVPDGRVGWVLSRMLAMAIPNEVAQYAEGARITSYFSLGKVLDGDVEKH
                     NWLWTTIRARDVSYEYDSFRIFIWNLRRHRYETAYIERNLEGYFPTRVHTVKVTEGGR
                     TVEVPGFTVITRNKDGQVYERRFAFQGYLVREVERVTSQIPTPPWEEVAEPQAAAPAV
                     SGTPRPASWFSRTKQWLDK"
     gene            complement(5901..6665)
                     /locus_tag="VFQ79_17825"
     CDS             complement(5901..6665)
                     /locus_tag="VFQ79_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0141588.1"
                     /translation="MAEQYPHYIEFRHVYKTFDYPVLVDANFYVDGGETVAIIGRSGV
                     GKSVALAHIMGFLKPDQGNVIVAHQDITGFREAELREIRKKVTMVFQSGALFDSLTVA
                     ENVLFSLELRDDYDEQNREDVVMGLLRLVGLEDAAEQYPSDLSTGYKRAVAIARALAA
                     QPECILYDEPTTMVDPIMSDHVASLMQRLKEQLHLTSVVVTHDLDLMRAVADKVVVLH
                     DGGVIYFGAVSELANSDHPHIREFLAMDRTLLGSNG"
     gene            complement(6658..7242)
                     /locus_tag="VFQ79_17830"
     CDS             complement(6658..7242)
                     /locus_tag="VFQ79_17830"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU0141589.1"
                     /translation="MRQAGFVLVGGRSSRMSRDKALLDYGGKTLAQHVAAEVLLAAGS
                     VTLVGAPERYSHLGFPVIADEFPETGPLGGIVTALGSSTAEWNLVLACDMPGVRAELL
                     SRLLDHAAASGADCVVPLSPSGRPEPLCAVYRRTCNALFRTALASGVRRIASALENVR
                     PEHIGFDAGGWFENVNTPADWHAHLTHCTGHTDG"
     gene            7395..8099
                     /locus_tag="VFQ79_17835"
     CDS             7395..8099
                     /locus_tag="VFQ79_17835"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0141590.1"
                     /translation="MALLCIGGSVLPAASHNDAARVTSVVRTDQKSGRLVRRVVAKPA
                     PVRAIPAKTTTGAAGSGIIDIPRIVELTAAKYDLDPLLVHSVIQAESNYDPFAVSPKG
                     AQGLMQLMPATAKRFGVQNVFRAEQNIEGGVRYLRYLHDLFRNPTLALAAYNAGEGAV
                     LKYNNTVPPYAETRSYVRRVAGKLDRARSARESGASEPAEVTKSETEGAPAEDAAYRP
                     VVSFVDGAGRLYLKTE"
     gene            8107..9423
                     /locus_tag="VFQ79_17840"
     CDS             8107..9423
                     /locus_tag="VFQ79_17840"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0141591.1"
                     /translation="MASVSSPNRSQWKWLKAAAAVWFFIPAPLLNARDLAVSAIRFWP
                     LGDVTRVAIEITGEFEYRYDRVYNPDRLYFDILGAQSLLQKRGTHVIPVGDRLLKQIR
                     IALTQPNVTRVVLDLEPSVDFICSQLANPNRLMIELRTAAAGGKLSSGTPAEPVAVQQ
                     EPPATPAPPPTPSDAVEASVKPPASAAPARVGGAGQQSLTRVLGLKVARIVIDAGHGG
                     HDTGTIGPGGLMEKDVVLDIAKRLGTLIQTKLGSEVVYTRSDDTFVALEERTKLANEK
                     KADLFLSIHANSSRLRSIAGVETFYLNLTSSKEDLDVAARENAASQQSIFELQNVIQK
                     IALTDKIEESREFAANVQKALYSALSRRNSRVRNRGVKKAPFIVLIGAQMPSILTEIG
                     FISHPREEALMKRPEHRQKIAEAIFKGLSAYASTLSHFEVAQTTTR"
     gene            complement(9413..>10258)
                     /locus_tag="VFQ79_17845"
     CDS             complement(9413..>10258)
                     /locus_tag="VFQ79_17845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0141592.1"
                     /translation="DILLREDSPRLAVLDWMMPGFTGPDLCKLVRQLKREPYTYILLL
                     TSRSLKGDLIEGMEAGADDYLVKPFDEHELKVRLRAGRRIVELNAELFEIREALREQA
                     TRDSLTGLWNRHAILEILARELERTRREAKPVGLVMLDLDHFKQVNDGYGHLTGDQVL
                     RESSARMQASVRPYDAVGRYGGEEFLIVLPGCDAVATAQHAERIRRVIASGSIPTSAG
                     EIWLTASVGATAAFESDCATNPEQMLRTADLALYDAKGRGRNCVAFRPVHYAPEPETE
                     SFLSA"
BASE COUNT         2075 a         2996 c         3150 g         2037 t
ORIGIN      
        1 ggggctggta cagcgccggg agttcgggat atcgggcggc cgtttccttc aggaccgcat
       61 ggatcgtgcg gtaagacatt ctcagtaaac ccacaaggta aaatgaaccg gatgcgcttc
      121 gaaaaagatc tccaagcggc acgggatgcc gcgatccgag ccggtgagct agcgctcatc
      181 catcgtagca aaggccttgc gcccgaggat aaagccgacc tttcgccggt taccatcgcg
      241 gatcgcgagt cggagcggct gctggtgacc gctctcaccg gagcttttcc ggatgacgga
      301 atccttggcg aggagggtgc caatcatacc ggaagctcgg ggcgccactg gattatcgat
      361 cccatcgacg ggacccgcga gttcctgcga gggagttcgg cctggtgcgt gctggttggc
      421 ctggaagcgg aaggagaggt ggaactcggc gtggctcatt tcccggccat gaagaccacg
      481 ttctgggcgg tacggggcga gggcgccttc cacgatgggg agcggatctc cgtatcctcc
      541 atctccagtg cggaccgcgc cgtattgtgt ttgaacggct tcaataatct tggcaaacac
      601 tcctttcagg acaggcttct tccattcctc tcgcgctatt ggtctgtgag gagcctcggc
      661 ggatgtctgg acgcggtgat ggtggcgcaa gggcaggccg acgcctggat tgaacccagc
      721 gcacagccgt gggaccttgc tgcactgaag gtgatcaccg aggaggccgg tgcgcgcttc
      781 ttcaatttcg acggcggcag cagcatccac gccggaaatt gcgtcacctg cgtaccggcg
      841 ctggaggcgg aactgcgggc gtttgtccgc aacgggtagg ctatgcgtgc tgtcgttctt
      901 ctgctcgccg gtatgccgtt tgccggtttt gcgcagagtc cggcaactct ttatgccgaa
      961 gcgcgagcac tggcggacac gaacacgccc gaagccatcc gccgcctcga ggacatcgtg
     1021 caacgctggc ccgagttcgt cgaagcacat ctttcgctcg ggcgaatcta tctcacccct
     1081 ggttttcgga accgcgaaaa agctatcgaa cacttgcagc gtgcgatgga tctacagccg
     1141 gatagcctgg agccgtacag actgctgacg gctcttcccc catcgagttt tttgagccag
     1201 agtgcgcggc ggatgcggga gttgcttgag agaagtacgg acccggacga tcttgcagca
     1261 ttcccggtcc tgtgggcgat cgagtttcgt accactccgc cctccggaca cagcgccgtg
     1321 cgcgaccgct tgaaagagga tgtggagcgg ctgaaaacag caggttcgcg gccgtccccg
     1381 gccgttctat atgcgttaag cgaggcacgc cgcatactgg gaaccgccga aacaccaggg
     1441 atgggttgga ggcaaatgtt gaaaccgctg cctgccttcc gattgccgga tcttgccgga
     1501 cgagtctgga cgccggacga tctcaaaggg aaagtaacgc tcatcaacgt gtgggccacc
     1561 tggtgcggac catgccgcgt cgaactgccg cacatccaga gactgcacga gaaagtgaaa
     1621 gaccgtacgg accttcttgt gctgacgttg aacgtggatg gccatcccgg ccgcgtggat
     1681 tcctatctga aggagggtgg gtacacgttt ccggtgatac ccgccgggga ttacgttctg
     1741 gaagcgacgc ccgtctttgt ggttccgcaa acctggattg tcgacggttc aggtatgatc
     1801 cgcgaacaat cggtgggatt cgccggggcg gatgagggct gggtagatga gatccttcgc
     1861 cgcatgaaac aatggaaata gtatccgtac agggcgcgtt tccgctacaa ttgaaccaat
     1921 aaccggcagg aatcctaatg catccgttgg ccacgaagcc gccctgcgta atcgcgatct
     1981 acccagggtc ttttgatccg atcaccaacg ggcatctgga cctgatcgag cgttcggcaa
     2041 gactggttga caagctcatc atttcgatcc tgcggaatga gaacaagcag ccgctgttca
     2101 gcgtacagga acgcatggac atgttgcgcg atgtagtggg tccctatccg aacgtggaag
     2161 tggattcgtt tgacggactg ctggtagatt atgccgcggc gaggagcgcg acggtgattc
     2221 ttcgcggtat ccgggccatt tccgactacg agtatgagtt gcaaatggcg ctgatgaacc
     2281 ggcggcttcg tccgcagatc gagaccgtgt ttttaatgtc cggagaggca tattccttca
     2341 tcagttcgcg gctggtaaag gaagtcttcc ggctgggcgg cgatatctcg ggacttgtgc
     2401 cgcccccggt ggaacaacga ctgaagaaca gatttctggg agagaaatag caattcatgc
     2461 aagcagcttt ggaactggcg gaaagagtat ccctgatcag tgtgtcctcc acgatgaagg
     2521 tggcggcgga cgctgatcgt ctcaggcggg aaggtgtgga cgtcgtcgat ttcggggccg
     2581 gggaacctga ttttcctacc cctgaaaaca tcaaacaggc cgcgattcag gccattgccg
     2641 aagacttcac caagtacacg gcggcgggcg gcacaccgga actgaagcag gctatctgtg
     2701 agcgtcaccg ccacgattat ggcaccgcgt attccccggc ggaatgcatt gtttcggtgg
     2761 gcggcaagca cgtgatcttc aacctgatgc aggtgttgtt gaacgcgggc gacgaagtgg
     2821 tgatcccggt tccttactgg gttacataca aagatgtggt gaactacgcc ggcggcaagt
     2881 gtgtctttgt cgaaaccgac gaggccgccg gcttcgcgct gacggctgcc atgctggagc
     2941 cgtacatcac cgataagacg aagctgatcg tcatcaactc gccctccaac cccagcggcg
     3001 ccgtgctgga tcgcgaggag ttcgaacgga tttaccacct ggccagccag cgcggagtgt
     3061 ggctgttgac cgacgaatgc tattgccgtt tcctgtacga gggcgatccg ttctccatcg
     3121 cggccatgcc gggggcgaag gaccacgtgc tggtcgccgg ttcgctttcg aaaacttatg
     3181 cgatgacggg ttggcgcatt ggcttcggcc tggccccggc gtccattatc ggcgccatca
     3241 acaagctcca gagccacagc acttccaatc cgacatcgat cgcgcagaag gcggcggtgg
     3301 aagcgctgcg tggcccgcag gactccgtcg gagtgatgct cgatgagtac cgcacacgca
     3361 gggatttcgt tctcaaacgc ctccggcaga ttccgggtgt cacgatccag gagccgaagg
     3421 gtgctttcta cgcctatccc aacatcggcg ccgcgcttgg aaagaacggc attcggaccc
     3481 cgatgcagtt cgcagagaag cttctggggg aggctcatgt cgccgttgtt cccggagagg
     3541 cgttcggcac ggaccggcat gtccgtattt cctacgccgc gtccctcgat gaattgagcc
     3601 gcggtctgga ccgcatgcac gaattcattg ccaagatctg aatgccggta caaaaactga
     3661 cgccttcctt ccgggagaag gtatggggcg caacggacct caccccctgg tttcccgttc
     3721 agcaaaccag gattggtgaa gtgtggtttg aatcggagag gccgctgccg ctgctggtga
     3781 agtttctctt cacgtcggaa cggctctcgg ttcaggtgca tcccggcgac gactacgcgc
     3841 gcaagcacga gaacagcccg ggcaagaccg aaatgtggca cgttctgcgt acggagggcg
     3901 acgcccggat cgcgctcgga tttcacgaac gcctgagccg tgagacgctg cgccgtgctt
     3961 cggaaaccgg cgagatcgag catctgctga actgggttcc ggtgcaggcg ggggactcca
     4021 tcttcgcacc cgccggaacg gtacacgcca tcgggccggg actcgcgctc tgcgaaatac
     4081 agcaggtctc cgatatcacc taccggttac acgactatgg ccgtccccgc gagctgcatt
     4141 tgaataaagc gatggatgtg gccaatacgg aaccccatcc cggaaaagcg gttccccggc
     4201 cgctgccgga tggatccctc ctgctggtct gctgcgaata cttctgtacg gaaaaggcgg
     4261 ttgtttccac cgaggttcct tggcagcctc gtgggagcaa cccggagatc ctggtggttc
     4321 tcgaaggcac gggctacttg gaaaacaaag tgtttggtcc aggcgaggcg tggctggtgg
     4381 acgagcccgt tgtccttcag ccccagggaa cggccgtgct gttgcggaca tacgttccgt
     4441 ccgccgcctg aacgggcttc tcctcagccg atgcccaggt cgtcggtgcg ttccagcgga
     4501 gtgccgcaat cgcggcagat cactgcgaga cagtcgccgc acaccagcgg ctcctgaacc
     4561 ggcttggcgc acttggggca gtacaggtct tcctgttgtg gcgccgccgg atctggagct
     4621 tcctgataca ctgaagttgt cagccctcta ttcgcacgag aatgccgctg tctcgtcttc
     4681 ttctatcctc tctcattgtg gcaacgctgt cgctcgcggg ctgcggttcc cgcgacgagg
     4741 gtcccaagcc gatcgggcac gctttcgtcg ctcaacccga cctgaaactt cgggctgagc
     4801 tcgatgcgaa agccccggcc accgccaccg tgcagaacgg ggaacgggta gagatcctgc
     4861 gaaccttccg ccggttcgtg aaagtccgga cgaagacggg tgcggaaggc tgggtgcacg
     4921 agcgccaact gatgagctcg cgcgaaatcg acaagctcga ccgggcatcc gaacgcctgc
     4981 gggagctccc gtcgcagggg agcgccaccg tctatgccac tctgaacgtt cacatcgagc
     5041 cgcaccggca atcgcctacg ttttaccaga tcagagaaaa gacgcacgtc accgttctcg
     5101 gtcaccaact cgtacctcgc ggcccctaca aaccggaacc tctgctcccc cctccgccgc
     5161 ctaagccgaa agcggtgaag aagccgagcc gtccctccca ggctcagact ccgccgattg
     5221 tcacgccgga ccctccgggt ccgccggaag actggctgga gatctcgaaa tcccctctgc
     5281 ccccggagcc ggagcccgaa ccggaacaga ctcctattcc cggggaagac tggagcctgg
     5341 tgcgggtgcc ggatggccga gtcggttggg ttctgtcccg catgcttgcc atggcgattc
     5401 caaatgaagt ggctcagtat gcggaaggcg cgcgcatcac atcgtatttt tccctcggga
     5461 aggtgctgga tggagacgtt gagaagcaca attggctgtg gaccacgatt cgcgccagag
     5521 acgtttctta tgaatacgat agcttcagaa tcttcatctg gaacctgaga agacatcgct
     5581 atgaaaccgc ctacatcgaa cggaacctgg aaggctattt ccccacacgt gtacacaccg
     5641 tgaaggtgac cgagggcggc cgcacggtgg aggtaccggg gttcaccgtg atcaccagaa
     5701 acaaagacgg ccaggtttac gagcgcaggt ttgcctttca gggctacctt gtgcgtgagg
     5761 tggaaagggt gacatcgcag attccaactc ccccttggga agaggtagca gaaccccagg
     5821 cggcagcccc ggctgtttcc ggcactcccc ggcccgcctc ctggttttcc aggacgaagc
     5881 agtggctcga taagtgagcg ttagccgttc gaacccaaca gggtacggtc cattgcgagg
     5941 aactcgcgga tgtgcggatg atcggagttg gcaagttccg aaacggcacc gaaataaatg
     6001 acgccgccgt catggagtac aaccaccttg tcggcgaccg ccctcatcag gtccaggtcg
     6061 tgtgtgacaa cgacggaggt caggtgaagt tgttctttca ggcgctgcat gaggctggct
     6121 acgtggtccg acatgatggg gtcgaccata gttgtcggtt cgtcgtacag aatgcattcc
     6181 ggctgtgcgg ctaacgcgcg ggcgatggcg acagcccgtt tgtagcctgt agacaggtcg
     6241 ctcgggtact gctcggcggc atcctccagg ccaacaagcc gcagcagtcc catcaccaca
     6301 tcctcacggt tttgttcatc gtagtcgtcg cgcaactcca gggaaaagag gacgttctcg
     6361 gcgacggtga gcgaatcgaa aagagcgccc gactgaaaaa ccatcgtgac ctttttccgg
     6421 atttcgcgca gttccgcctc ccggaatccg gtaatgtcct gatgcgccac gatgacgttg
     6481 ccctggtcgg gcttcagaaa gcccataatg tgcgccaggg ccacagactt tcccacgccg
     6541 ctgcgcccga tgatggcgac ggtctctcct ccgtcgacgt aaaaattggc atcgactaaa
     6601 acggggtagt cgaacgtctt atacacgtgc ctgaactcga tgtagtgagg atactgctca
     6661 gccatccgtg tgcccggtac agtgagtgag atgggcgtgc cagtcggccg gggtgttaac
     6721 gttttcaaac catccgcccg cgtcaaaacc gatatgctcc ggacggacgt tctcgagcgc
     6781 ggaggcgatt ctgcggacgc ccgacgcgag cgccgttcga aagagcgcat tgcaagtccg
     6841 ccggtagacc gcgcacagcg gttccggccg tcccgacgga gagagcggta cgacgcaatc
     6901 cgcgccggag gcagcggcat gatccagcag acgcgaaagc agttctgccc gcacgcccgg
     6961 catatcgcag gcgagtacca ggttccattc ggccgtggag gacccgaggg cggtgacgat
     7021 accgccgagc ggacccgtct ccgggaactc gtccgcgatc actggaaaac ccaaatggga
     7081 ataccgttcc ggagctccga cgagcgttac cgacccggcg gcgagcaaga cttccgctgc
     7141 gacgtgttgg gcaagcgtct tgcctccgta gtcgagcagc gctttgtcgc ggctcattct
     7201 ggaactccgg ccgccgacca gaacgaaacc agcttgccgc atgcagagat gatagcattt
     7261 gcgataacga agcccgttca ggtaattatt atcttcaata tcagtgaact ggctgaaccg
     7321 gcacggcttg gtgtcgcgaa cgagtggggg ttataatcgg tttgaacaaa gagatgcgga
     7381 attgtttggc atccgtggcg ctattgtgca tcggtggttc ggttcttccg gcggcgagcc
     7441 acaatgacgc cgcccgcgtt acatcggtcg tgaggaccga tcagaagagc ggccggctgg
     7501 tgcgcagggt agtagcgaag ccggcgccgg tgcgcgcgat tccagcgaaa accaccactg
     7561 gtgccgcggg ctccggtatc atcgacatcc cgcggatcgt cgaacttacc gcggcaaagt
     7621 acgatctcga tccgttgctc gttcattcgg tgattcaggc ggagagcaac tacgatccat
     7681 ttgccgtctc tcccaaggga gcgcaggggt taatgcaatt gatgccggcc accgcgaagc
     7741 gtttcggcgt ccaaaatgta tttcgcgcgg agcagaacat cgagggcggt gttcgatatc
     7801 tccggtatct gcacgactta tttcggaacc cgacgctggc actggcggct tataacgccg
     7861 gtgaaggcgc ggtgttgaag tacaacaaca cggttccgcc gtatgcggag acccggagct
     7921 atgtccggcg ggtagccgga aaattggacc gagcgagaag tgccagggaa tccggcgcat
     7981 cggagccggc agaagtgacg aaaagcgaga cggaaggagc gcccgccgag gatgctgcct
     8041 accggcccgt ggtcagcttt gtggacggcg cgggcagatt atatctgaag acggaatagg
     8101 acggtaatgg catcggtttc ctcccccaac cgatcgcaat ggaagtggct gaaagccgcg
     8161 gcggcggtct ggttcttcat cccggcgccc ttactgaatg cacgggacct ggccgtgtcc
     8221 gccatccggt tctggccttt gggcgacgtc acccgggtcg ctatcgagat caccggtgaa
     8281 ttcgagtacc ggtatgaccg tgtatataac cccgaccggc tgtactttga catactcggc
     8341 gcccaatccc tgttgcagaa gcgtggcacc cacgtcatcc ctgttggcga ccggctgctc
     8401 aaacagatcc gcatagctct cacgcagccg aatgttacgc gggtggtgct cgatttagaa
     8461 ccctcggttg atttcatctg ctcccagcta gccaatccaa accggctcat gattgaattg
     8521 cgcacagccg cggccggcgg caaactctcg tccggaacgc ccgccgagcc ggttgccgtg
     8581 cagcaggagc ctcccgcaac tccggcgccc ccgccgactc catcggacgc ggtggaggcg
     8641 tctgtcaaac cgccggcgtc cgccgctccg gctcgcgttg gcggtgctgg acagcagtcg
     8701 ctgacacgtg tgctcggcct caaggtggcc cgtattgtga ttgacgccgg acacggtggc
     8761 cacgacacgg gaacgattgg ccccgggggc ttgatggaaa aggatgtggt gctggatatt
     8821 gcgaagcggc tcggcacgct tatccaaacg aaactcggta gcgaagtggt gtacacccgg
     8881 agtgacgaca cgtttgtcgc gctcgaagag cgcaccaagc tcgcaaatga gaagaaggcg
     8941 gatctgtttc tgtcgattca tgccaactcc tcgcgtcttc gttccattgc cggcgtcgaa
     9001 accttttatt tgaatctcac ctcttcaaag gaggatctcg atgtggcggc gcgcgagaat
     9061 gcggcatcgc agcaatccat ctttgagcta caaaacgtga ttcagaagat cgctcttacg
     9121 gataagatcg aggagtcccg tgaattcgcc gccaacgtcc aaaaggctct ttatagcgct
     9181 ttatcgcgga gaaactccag ggtgagaaac cgcggtgtca aaaaggcgcc gttcatcgtg
     9241 ctgattggcg cgcaaatgcc gtcgattctg acggaaatcg gcttcatcag ccatcctaga
     9301 gaggaggctt tgatgaagcg gccggagcac cggcagaaga ttgccgaagc gatttttaaa
     9361 gggctatcgg cctacgcttc cacattgagc catttcgagg tggctcagac cactacgcgc
     9421 tgaggaagct ttccgtttcg ggttccggcg catagtgcac cggacggaac gctacacagt
     9481 tccggccgcg gccttttgca tcatagagag ccaggtcggc ggttcgcagc atctgttcgg
     9541 gattcgttgc acaatccgat tcgaaggcgg ctgttgcgcc tacgctggcg gttaaccaga
     9601 tctctccggc ggaggttggg atggagccgg atgcaatgac cctccgtatg cgttcggcgt
     9661 gctgcgccgt agcaacggca tcgcagcccg gcagcacaat cagaaattct tctcccccgt
     9721 agcgtcctac ggcgtcgtaa ggccggacgg acgcctgcat gcgcgcggac gattcgcgca
     9781 gaacctgatc tcccgtgagg tgcccgtacc cgtcgttgac ctgcttgaaa tggtctagat
     9841 ccagcatgac aagaccgacc ggctttgctt cgcgccgtgt tctttcgagt tcacgagcga
     9901 gaatttcgag aatggcatgc ctgttccaca gccccgtgag cgagtcccgc gtcgcctgtt
     9961 cccgcaaagc ttcacggatt tcaaacaatt cggcgtttag ctccacgata cggcggccgg
    10021 cgcgaaggcg aaccttcagt tcgtgctcgt caaagggctt caccaggtaa tcgtctgccc
    10081 ctgcctccat tccttcgatc agatcgcctt tcaggctccg cgaggtcagc aacaggatgt
    10141 aggtgtaggg ctcccgtttc aactgacgca ccagtttaca gagatcaggt cccgtgaagc
    10201 cgggcatcat ccaatccagc acggcgaggc gtggagaatc ctcccggagg agaatgtc
//
LOCUS       DASTMK010000415         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_309407, whole genome shotgun sequence.
ACCESSION   DASTMK010000415 DASTMK010000000
VERSION     DASTMK010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309407"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="VFQ79_17850"
     CDS             <1..1577
                     /locus_tag="VFQ79_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141593.1"
                     /translation="RERAPNGVQADLSIRGAGFGQTLVLLNGQRLNDAQSGHHNMDIP
                     VPLESVARIEVLRGAGSTYYGSDAVGGVVNVITDEPEYSEFRLRSAVGNHGVNQQQGF
                     IALGGGPISQQFSFARDFSSGFRANRDYRNLSFASTTGVSSRYGDTRLLAAYNDRPFG
                     AEGFYGNYPSWERTRTWFGSVQHSFPSDTQVAVAYRRHTDLFVLYRDRPEVFTNHHAA
                     ESYQAAVRQRAPIGLNAALHFGAEAFGDRISSTNLGRHGRTRGAGYIAFDARALQRFS
                     LSLGIRDEIYGSGNHEASPNAAIGFWVSPQLKLRAGVSRAFRLPSYTDLYYHDPANLG
                     SPDLLPESAVSYEAGADWRPSQAVGGDVTVFHRRETDVIDYIRLSLADIWRATNFHRL
                     RFTGAEAALRLRLARTQQIDLRYTALRGVASLPPAIQTKYVFNYPVHSGVVSWLTPLP
                     KNSMLRVRVGVLERLARDPYTLVDIYLAAKPGRIQPFVQVTNMAGSRYEEIAGVAMPG
                     RAILGGVQLVVCCRPK"
     gene            1613..3103
                     /locus_tag="VFQ79_17855"
     CDS             1613..3103
                     /locus_tag="VFQ79_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141594.1"
                     /translation="MIRTLLAILLAVLCASNAVPAEQGQLDASPTLFSVLAAINAAGY
                     DADLDSPANDPIREAVRKNLASKNIPCLNDLKQFFASHRQKDWTAELSQYVSFALSVD
                     GPPAFEYRFQHYLLPPDVQPLTGFETLMARFHREADIDTLWQQAQSAFERQIARYHQP
                     VSAAVLEVNGYMRNTSGGFLGRRFQIYIDLLGAPNQIHTRSYADDYFVVLTPSPEPQV
                     EDVRHAYLHYLLDPLATKYGMVLEEKKPLIDFAFGAPYLEEYYKNDFLLLTTESLIRA
                     MESRLERGPAEKKNQMVAQALGEGFILTPYFAEQLPAFEQQDQAMRLYFPEMIKAIEL
                     KRESKRLENVQFAATRPVRKAKVVVPAERKVEPTGVFKTLEEAEQSYTGRNLDSARAA
                     YLKVLEETDQKPLHAKAYYGLARIAALKNDPELAEKLFQQILELSPDPQTAAWAHVYL
                     GRLSDIAGDREAASGHYHAALRVQGASNAARKAAEQGLKAEFPKNN"
     gene            3148..3972
                     /locus_tag="VFQ79_17860"
     CDS             3148..3972
                     /locus_tag="VFQ79_17860"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141595.1"
                     /translation="MKNRVWRAVMGLMAGAGLLLAQEAAPVAKQPQPKSKQEVEALQA
                     MFSAQDPDGRIAAAQNLITKFADTEFKALALQLIAMSYEQKGDADNMIVYSERVLEAD
                     PQNYASMLMISRALAQRTKEFDLDKEEKLGRAEKMAHQAQEALKTTPKPRPDITDDQW
                     AEAKKDFNSQALESLGLIAMVRKKYDVAIKEFNAAIEGSSNPDPATMVRLGAVYNLAG
                     KHDEAIGILDKVMALPEVHPQIKQIAQAERVRAMQAKGGAKPATPPPAEPAKPANP"
     gene            3914..>4684
                     /gene="rnc"
                     /locus_tag="VFQ79_17865"
     CDS             3914..>4684
                     /gene="rnc"
                     /locus_tag="VFQ79_17865"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU0141596.1"
                     /translation="MEPSPQHRPPPSPQSLPIRNLLSKRISADPEGLERALGYSFADK
                     TLLVRALTHRSRAYEQSSVSGPAADNEQLEFLGDSILGFIVSDYLLAHFPSFREGQFS
                     VWKARIVSAANLAQVARRIQLGDFLVLGKGEQSSGGRSKGALLSNAIEALIAAVYLDG
                     GMGAARTLVERLVIGNFNPSEAAAENVNAKTALQEKSHALGLPLPRYVVVREEGPPHE
                     RTFTVEARIGTRYSSSAQGLSKKSASQSAAELLLTQLER"
BASE COUNT          992 a         1357 c         1408 g          927 t
ORIGIN      
        1 tgcgtgagcg cgcgcccaac ggcgtgcagg ccgacctctc catccgcggc gccggcttcg
       61 gccagacgct cgtgctgctc aatggccagc gcctgaacga cgcgcagtcg gggcatcaca
      121 acatggatat cccggtgccg ctcgaatccg tggcgcgaat cgaggtgctg cgaggtgccg
      181 ggtcgacata ctacggctcc gatgcggtcg gtggagtcgt taacgtaatt accgatgagc
      241 cggagtattc ggagttccgt ttgcgaagtg ccgtaggcaa tcacggagtg aaccagcagc
      301 agggctttat tgcgctcggt ggcggaccca tttcccagca gttcagtttc gcgcgcgatt
      361 tctcgtcggg cttccgcgct aatcgcgatt accgcaatct gtcgttcgct tccaccacgg
      421 gtgtgtcttc gcgctacggc gatacgcgtc tgctggcggc atataacgac aggccgtttg
      481 gtgcggaagg tttttacggc aactacccgt cctgggagcg gacacgcacg tggttcggct
      541 ccgtgcaaca ttcgtttccg tcggatactc aagtcgctgt tgcctaccgg cggcacacgg
      601 atctattcgt gctgtaccgc gatcgtcccg aggtattcac caatcatcac gccgccgaaa
      661 gttatcaggc cgcggtccgg cagcgggcgc ccatcggcct gaacgcagct ttgcatttcg
      721 gtgcggaggc gttcggcgat cgcatttcga gcacgaacct cggaaggcat ggcagaaccc
      781 ggggggccgg ctacattgcg ttcgatgcgc gagcgcttca gcgcttctcg ctttctcttg
      841 ggattcgcga cgaaatatac ggatcgggga atcacgaagc tagcccgaac gccgcaattg
      901 gtttttgggt ttcgccgcaa ctgaaactgc gtgcgggcgt gagccgcgcc ttccgcctgc
      961 catcctacac ggatctgtac taccacgatc ccgccaacct gggctctcct gaccttttgc
     1021 ccgaatccgc cgtcagctat gaggctggcg ccgactggcg gccgtcgcag gctgtgggcg
     1081 gcgatgtgac ggtctttcac aggcgcgaaa ccgatgtgat cgactacatc cggctctccc
     1141 tcgcggacat atggcgagct accaattttc accgcttgcg gttcaccggc gcagaagccg
     1201 cacttcggct gcgtctggcg cgtacgcaac aaatcgacct ccgctacacg gctctccgtg
     1261 gagttgcgag ccttccgccg gccattcaga caaagtatgt tttcaactac ccggttcatt
     1321 cgggcgttgt gtcgtggctc acgcctctgc cgaaaaattc gatgttgaga gtgcgggttg
     1381 gcgtgctgga gcggctggcg cgagaccctt acacgctggt cgatatttac ctggcggcga
     1441 agcccggacg tattcaaccg ttcgtgcagg tgacgaatat ggccggctcc cgttatgagg
     1501 aaatcgccgg ggtggcgatg ccgggcaggg ccatcctggg cggggtgcaa ctcgtggttt
     1561 gttgccggcc gaaataagct agactgaaaa taacggtgca aggcaggatt tcttgattcg
     1621 cactctcctc gcaatactgc tcgccgttct atgtgcgagc aatgcggtgc cggccgagca
     1681 aggccagttg gacgccagcc caaccctgtt cagtgtactc gccgcgatca acgccgccgg
     1741 ctatgatgcc gatctcgact cacccgcgaa cgatccgata cgcgaggcgg tccggaagaa
     1801 cctggcgtcc aagaacatcc cgtgtctcaa tgatttgaag cagtttttcg cctcccatcg
     1861 ccaaaaggac tggaccgccg agctgagtca gtatgtctcc ttcgccctga gcgtggatgg
     1921 ccctcctgcg ttcgaatacc gcttccagca ttacctgttg ccgccggacg tacagccgct
     1981 gacggggttc gaaacgctga tggcgcggtt ccatcgcgag gcggacatcg acacgctctg
     2041 gcaacaggca caatccgctt ttgaacggca gatcgcccgc tatcatcaac cggtgagcgc
     2101 cgcggtgctg gaggtgaacg gctacatgcg caatacctcc ggcggattcc tgggccggcg
     2161 gttccagatc tatatcgacc tgctgggcgc gccgaatcaa atccacacgc gcagctacgc
     2221 cgacgactat ttcgtcgttc tgacgccgtc tccggaaccc caggtagagg acgtgcggca
     2281 cgcctacttg cattacctgc tcgatccgct tgccacgaag tacggtatgg tgctggaaga
     2341 gaaaaagcct cttatcgatt tcgcctttgg tgcgccatac ctggaggaat actataagaa
     2401 tgactttctg ctattgacga cagaatctct gattcgcgcc atggaaagcc gcctggagcg
     2461 aggtccggcg gaaaagaaaa atcaaatggt agcgcaggcg ctaggggaag ggttcattct
     2521 gacgccgtat tttgccgagc agttgccggc gtttgaacag caggatcagg ccatgcgtct
     2581 ctacttcccc gaaatgataa aagctatcga attgaagcgg gagtcgaagc gcctggagaa
     2641 tgttcagttc gcggcaactc gtcccgtgcg gaaggcgaag gtggtggtgc ccgccgaacg
     2701 aaaagtggag cccacgggtg tcttcaaaac cctcgaggaa gcggaacaat cgtacacggg
     2761 acggaatttg gattctgcgc gtgcggcgta tctcaaagtt ctcgaggaaa ccgaccagaa
     2821 gccgctgcac gccaaagcat attacgggct ggcccgcatc gccgccctta aaaacgatcc
     2881 cgagttggcg gagaaactat ttcaacagat tctggagctc agtccggacc cgcagacagc
     2941 agcctgggct catgtgtatc ttggccggct gtccgacatc gcgggtgatc gggaagccgc
     3001 ctccgggcac taccacgccg ctcttcgagt ccagggcgct tcaaacgcgg cacgcaaagc
     3061 ggccgaacaa ggtttgaagg ccgaattccc caagaacaac tgataacatc aataggttaa
     3121 gtcgacgcag gaaaggaatc ttttgcaatg aaaaatagag tttggagagc agtcatgggc
     3181 ttgatggcgg gcgcggggct attgctcgcg caggaagccg cgcccgtcgc gaagcaacca
     3241 caaccgaaat cgaaacagga agtggaagcc ttacaggcga tgttcagtgc gcaggatccg
     3301 gatggccgga tcgccgccgc gcagaacctg atcaccaagt tcgcggatac cgagttcaag
     3361 gcgctggcat tgcagttaat cgccatgtct tacgaacaga agggagatgc ggacaacatg
     3421 atcgtctatt cggagcgcgt gctggaagcc gatccgcaga actatgcatc catgttgatg
     3481 atctcccgcg cgctggcaca acgaacgaaa gagttcgatc tcgacaagga agagaaactc
     3541 ggacgggcgg agaagatggc gcatcaggct caggaggctc tgaagactac gcctaagcca
     3601 cgcccggaca ttaccgacga ccagtgggcg gaggcaaaga aggacttcaa ctcccaagcg
     3661 ctggaatccc tcggactgat cgctatggtg cgcaaaaagt acgacgtggc gatcaaagaa
     3721 ttcaatgcgg cgatcgaagg ctcaagcaac cctgatcccg ccacgatggt aagacttggc
     3781 gccgtttata accttgccgg caaacacgac gaagcaatcg ggatattgga caaggtaatg
     3841 gcgctaccgg aagtgcatcc gcagattaag caaatcgcgc aggcagagcg cgtgcgtgcc
     3901 atgcaggcca agggtggagc caagcccgca acaccgcccc ccgccgagcc cgcaaagcct
     3961 gccaatccgt aatttgcttt ctaagcgaat aagcgcggat ccggaagggc tggagcgagc
     4021 cctcgggtat tctttcgcgg acaagaccct tctggttcgc gctttgacgc atcggtcccg
     4081 ggcttacgag cagagttcgg tgtccggtcc tgccgccgat aacgaacaac tcgaatttct
     4141 gggcgattcc atccttggct tcatcgtcag cgactatctg ctggcgcact ttccatcatt
     4201 tcgcgagggg caattttccg tttggaaggc ccgcattgtg agtgcggcca atctggcaca
     4261 ggtcgcgcgg cgaatacaac tgggcgattt tctggtttta ggcaaaggcg aacagtcgtc
     4321 cggcggccgt tccaaaggag cgttgttatc caacgccatc gaggccctga tcgcggctgt
     4381 ttacctggat ggcgggatgg gcgctgcgcg cacgctggtt gaaagactcg tcattgggaa
     4441 tttcaatccg tcggaggcgg cggcggaaaa tgtcaacgcc aaaacggcgc tccaggaaaa
     4501 gtcccatgct ttgggcctgc cgctgccgcg gtacgtggtc gtgcgggaag aaggtccgcc
     4561 gcatgagcgc accttcaccg tggaggcacg cattggaacg cgatactcga gcagtgccca
     4621 agggctttcc aagaagagtg ccagccagag tgccgccgaa ttactgctca cgcagcttga
     4681 gcgg
//
LOCUS       DASTMK010000416        10132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_564034, whole genome shotgun sequence.
ACCESSION   DASTMK010000416 DASTMK010000000
VERSION     DASTMK010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564034"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFQ79_17870"
     CDS             <1..416
                     /locus_tag="VFQ79_17870"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0141597.1"
                     /translation="IALKPHTGITARGKDCLEVVKRLPSERLKICWDAGNVSFYEGVH
                     PDPDLVDLAPHVKAVCIKDHLGLRGEGNFPPPGEGQIDHELMFKTLFGAGFNGPMALE
                     RVDGRDNAAKMPAELIDQRIAAAQKFLTGILEKNA"
     gene            413..1438
                     /locus_tag="VFQ79_17875"
     CDS             413..1438
                     /locus_tag="VFQ79_17875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141598.1"
                     /translation="MKTLGVGIVGTGWVSDEYIKAFSKHEHCEVIGICSRDAQRARNK
                     IAEHKLANGEPFTDLSAMIQQNGLDVAVICTPHHLHVEQGVACAQAGKHVIVEKPIAI
                     DIAGVRRLDQAIRAAGVKSIVSFVLRWNPMFDNIRAMLERKVIGDIFYAEVDYLHGVG
                     PWYRIWEWMRKSEFGGSAILAGGSHAVDGLRWFVQDEAVEVTAYSNRSRSNPLQYEYS
                     PNTVTLVRFAGGAIGKVACTLECRMPYVFNVELFGDKGSIRNNKLFTTALPGQNDWAT
                     IPAIQPDSGDVSHHPFQQEVNHFVECIEKNVESHASVADAAKTHEICMAADLSAAEGR
                     PVKLPLQ"
     gene            complement(1445..2446)
                     /locus_tag="VFQ79_17880"
     CDS             complement(1445..2446)
                     /locus_tag="VFQ79_17880"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0141599.1"
                     /translation="MKSDPGGKRVPVGVLGMGLMGRSIVTCLLAAGHPVTGITTTASK
                     LTTTRRQVLALLKELEREGLLKTSPASVIDRLTVSTSYADLAGAEVVIESVIENIETK
                     KDLYRRVEEVVSRRCLIGSNTSAIPVSLLQEGLKYPERVLGFHWAEPAHITRFMEIIC
                     GKQTKPAFAKRAVELARGWGKEPSLLKRDIRGFITNRCFYALLREAFHLVDSGYATIA
                     DVDRSLRNDLGYWITFAGPFRFMDLTGIPAYEAVTKDLFPELDNRTSVPELMAKTVKS
                     GARGVSNAKGFYRYTPESAARWEKLFLKFSYDIRKLAMQYPEDIGDRPVRSRSRKKA"
     gene            complement(2443..2829)
                     /locus_tag="VFQ79_17885"
     CDS             complement(2443..2829)
                     /locus_tag="VFQ79_17885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEU0141600.1"
                     /translation="MAKTEIKHPDKTASTGAYSAGVVADGWLYVSGQGPLDMKTGAVV
                     EGSIEDQTRLTLEHVGKILNAAGCGFEDVVKCTCHLRDIADFDRFNQVYAQFFTGIRP
                     ARTTVQSVLWGTIKVEIDCIAKIPQK"
     gene            complement(2929..3765)
                     /locus_tag="VFQ79_17890"
                     /pseudo
     CDS             complement(2929..3765)
                     /locus_tag="VFQ79_17890"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(3865..4830)
                     /locus_tag="VFQ79_17895"
     CDS             complement(3865..4830)
                     /locus_tag="VFQ79_17895"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0141601.1"
                     /translation="MRNDLKNIRGILPVIPTPFTPEGEIAWEDFRALIDFAAAAGACA
                     ACLPAYASEFYKLTDEERSQLVYEAVSHSNGRLPIVGQVNTPSAASAVEAVESHQRAG
                     VAGLAVAVPRLFALPEDDLFRYFDRVLAPAQVPVIIQDFNPGGASVSPQFIVRLHERH
                     PQFRYIKLEEPMMAAKAARIRQESGGAVQVIEGWGGMYMLELIPAGISAVMPGVALTD
                     LLARVFELAGAGDVEEAYEIFAVVLPQIVFSLQNLELFHHAEKLLLRDRGIIRHAAVR
                     DAGLLLRPEDERHIRFLNGRILALLDRYALPANPLSIAAGNKALP"
     gene            complement(4840..6828)
                     /locus_tag="VFQ79_17900"
     CDS             complement(4840..6828)
                     /locus_tag="VFQ79_17900"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU0141602.1"
                     /translation="MRLPIALLLLPLAAGAAEHRPLLPQPREVRYGVGSLSLSGVSVR
                     VVPPASAEDQFAASVLTSAFSLDRGKGSGPEIILRRTPQQVGNSPEGYSIKVTPGGAE
                     VTAGSTAGLFYGVQTMRQLATGGALPEVEIRDWPALPYRGFMMDISHGPMPTEAEIKR
                     QIDFLSRWKANQYYFYSEASIEIDGYPLVNPDARYTKEELRRIIAYAKERHVDVVPCL
                     EFYGHLHDLFRVERYAQMAPVPYGGEFNPHHPGVRKVLEDWIGQLAALFPSEWFHVGL
                     DEPWALEQLGSKAAGVPPGQLYIEQLKTVSDIVRKNGKRMMFWADMVAGARIFTKYPE
                     LISQLPPGTIAVPWHYSPEKDYTPMVEPLRKAGVDVVVAPAVWNWNEIAPSFRTSFAN
                     IGGFLEAGKKEKAIGLLNTGWSDSGQVLYRMSLPGMAYGAAAPWQAAPMNSSSFFSEY
                     AALVYSGPTAADVATGLELLTESQEAFGKALGGGTIHSLWADPLAPRRLERAQAHSRE
                     LHQARLNAEEAQSHFRRALELKGEPSTIGPLLLGARMLDYLGMKYLYAAEMAGYFERL
                     GSKPDREAVYLLIRIQSASQNHGLIQDLLDGISELKQPYREAWLAEYRPYRLESALGR
                     WQAEYEYWRALQARFQEFGSSFKTGESLPPIEDFRPKR"
     gene            complement(6831..10010)
                     /locus_tag="VFQ79_17905"
     CDS             complement(6831..10010)
                     /locus_tag="VFQ79_17905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141603.1"
                     /translation="MRKILFLLLAAILLSLPMIAQLALSTIRGTTVDPSGALVANAEI
                     TVTNTQTNATRVAKTNENGDFEFPDLQRGTYELTAKASGFKAFVAQNIILDANQVRRI
                     NVTLEIGAVDAQIMVRADAAVIETDTAKLGGSVNTSRHFDNPWVGAEATLDPSLFITV
                     LPLVSQTNGVWSSQWAGQSSSQVQEGQDGHTNDNAVNQINDILDTQEITVVTVNNSAE
                     FARVGYMNMITKSGTNEWHGRAAYFHQNSAFNAREFFESQKAKTLIHTTSVSLAGPIK
                     RDKAFFYASANILKVPSKQFYLRDVPTDRMRQGDFSQLLGLSSPVIIRDPLTGDPFPG
                     NVIPANRISSVSARVNESYLPAPNRLGSDALASNYAFTFPFPTDYALRKDFTQRVDYH
                     LSSSNRLMGRVIENWGLYVLPTNFPQFAWTRVRFNVHTVIEDTHVFSPTLVNTARVGF
                     YKEKITDGDELYGVTPFKGDEAVQELGLQGVNRLGLSAQGFPRIDIAGYPTLRTQPGG
                     EVQNDHNWGFADTATWNTGRHVVRFGGEYKPQNRFRGNVPEGNYGSFNFNGSLTGYGY
                     ADFLLGLPFSSSRLDPLTNRKLTDSELGIFITDSWKVSNRLTLDIGLRWDRFGSATYD
                     DGLVYNWDPGTGNVIISQNAMDKVSPLYPATITLAAGNPRPNPSNTNFVPRIGAAYRL
                     SDKTVLRGGYGIFNETIGRYARAVTGGPFEITETYNNQIINGQPLLQFPNPFPSSLAG
                     ANIPSQSVTGFPRDTDNGKIHQFNLTVERQVGEVGLKLAYIGSRNRGMNYNISINKPE
                     ASLIPFTAARRPWSQFVGTTYARSDGKANYNAMLAEVQRKVGMLTLNAHWTWSSNYNT
                     TTVLQDPYAEPVWSRDPFTARHRVVVNTVWELPVGKGRRYLAGAPAVAEHILGGWQLY
                     WIAYFETGQFFSPSFSGRDPSNTNTVGGLPDRICDGNLPAGERSIDRWFDASCFAVPP
                     TGRFGNSGAYVLEGPGLHMHNISLAKTFDLTERFKFTLTGAASNAFNHANFAGPALNI
                     SAPGSVGIISDVRDGGRSRRIELRGRIDF"
BASE COUNT         2167 a         2948 c         2996 g         2021 t
ORIGIN      
        1 ccattgcgct aaagccgcac acgggcatta cggcccgggg gaaagactgc ctcgaagtgg
       61 tgaagcgcct gccttccgag aggctgaaga tctgctggga tgcgggcaac gtttccttct
      121 acgaaggggt tcatccggac ccggacctgg tggaccttgc gccccatgtc aaagcggttt
      181 gcatcaagga ccatttgggg ctgaggggcg aagggaattt cccaccgccg ggcgaaggac
      241 agatcgatca tgagctgatg ttcaaaacct tgttcggcgc gggtttcaac ggccccatgg
      301 ccctcgaacg cgtggatggc cgtgacaacg ccgcaaagat gccggcggaa ctcatcgacc
      361 aacggattgc ggccgcccaa aaatttctga ccggcatact ggagaagaac gcgtgaaaac
      421 acttggagta ggaatcgtag gtaccggctg ggtgtcggat gaatacatca aggcattctc
      481 gaaacacgaa cattgtgagg tgatcgggat ctgcagccgt gacgctcaac gcgcacggaa
      541 caagatagcg gagcacaaac tcgccaacgg cgaaccgttt accgatctct ccgcaatgat
      601 ccaacagaac ggtctcgacg tcgccgtcat ttgcacgccg caccatctgc acgtggagca
      661 gggcgtggcc tgtgcgcaag ccggcaaaca cgtcatcgtg gaaaagccga tcgccatcga
      721 catcgctggt gtgcggcgtc tcgatcaagc gatccgcgcc gccggcgtga agagtattgt
      781 cagtttcgtg ctgcgctgga atccgatgtt cgacaacatc cgggcgatgc tcgaacgcaa
      841 agttatcggc gatatctttt acgccgaagt cgattacctt cacggcgtcg gcccctggta
      901 caggatttgg gaatggatgc ggaagtccga gttcggcggt agtgcgatcc tggcgggtgg
      961 ctcgcatgcc gtcgacggct tgcgctggtt cgtgcaggac gaagctgtag aggtcacggc
     1021 ctattcaaac cggagccgga gcaatccact tcagtatgag tattctccga ataccgtgac
     1081 gctggtgcgc ttcgcgggag gcgcgatcgg caaggtcgca tgtacgctcg aatgccgcat
     1141 gccgtacgtg ttcaacgtcg aactgttcgg cgacaaagga agcatccgca ataacaagct
     1201 gttcacgacc gcgttgccgg gccagaacga ctgggccacg attccggcca ttcaacccga
     1261 cagcggcgac gtgtcgcatc atccctttca acaggaagtg aatcactttg tcgagtgcat
     1321 cgaaaagaac gtggagagcc acgccagcgt cgccgatgcc gcgaaaacac acgagatctg
     1381 tatggcagcc gatctctcgg ctgccgaggg ccggcctgta aaattaccgc tgcagtaaaa
     1441 cgtttcaggc cttttttcgg gaccggctgc gaacgggccg gtctccgata tcttccggat
     1501 actgcatcgc cagcttgcgg atatcgtagc tgaacttcag aaacagcttc tcccaccggg
     1561 cggcgctttc cggcgtgtag cgatagaagc ccttggcgtt cgatacgcct cgcgcgcccg
     1621 atttcaccgt tttcgccatc aactcgggga cgctggtccg gttgtccagc tcagggaaaa
     1681 gatctttcgt gacagcctca taagcaggga tgcccgtaag gtccataaag cgaaacggtc
     1741 cggcgaacgt gatccagtat ccgaggtcgt ttcgcaggga gcggtcgacg tccgcgatgg
     1801 tcgcatagcc ggaatccacg aggtggaacg cttcccggag cagcgcatag aagcagcggt
     1861 tcgtaatgaa accgcgtata tcgcgcttta acagcgaggg ctctttaccc catccacgcg
     1921 cgagctccac cgcacgtttc gcgaatgcgg gttttgtctg tttgccacag ataatttcca
     1981 tgaaccgtgt aatgtgcgcc ggctcggccc agtgaaaccc gagaacccgc tcgggatact
     2041 tcagcccctc ctgtagcagg ctgacgggaa tagcggacgt attgctgccg atcaggcacc
     2101 gccgcgacac cacttcttcc acacggcgat acagatcttt cttggtttcg atgttttcaa
     2161 taacggattc gatgacgact tcagcaccgg cgagatccgc atagctggtg gaaacagtaa
     2221 gcctgtcgat tacgctggcc ggcgatgtct tgagcaaccc ttcgcgttcg agttccttga
     2281 gaagcgccaa aacctgccgg cgcgtagtgg tcaacttcga cgcggtagtg gtgatgccgg
     2341 tcaccgggtg accggccgct agaaggcatg tgacgatgct gcggcccatg agccccatgc
     2401 cgagcacgcc caccggcaca cgcttgccgc ctggatcgga tttcatttct gcgggatttt
     2461 cgcgatgcag tcgatttcta ctttaatggt tccccagagc accgattgga cagtggtccg
     2521 ggccgggcga atacccgtaa agaactgcgc atacacctgg ttgaagcggt cgaagtcggc
     2581 gatgtcccgc aagtggcagg tgcactttac cacatcctcg aatccgcatc ccgcggcgtt
     2641 gaggattttg ccaacatgct ccagagtaag acgggtctga tcttcaatac tgccttccac
     2701 tactgcgccg gttttcatat ccagcggacc ttgtccactg acatacaacc atccatccgc
     2761 tactacgccg gcggaatagg cgcccgtgga ggcagtctta tcggggtgtt tgatttcggt
     2821 tttggccacg aagggatttt ggcgcagacg ggcacccgtc cgcaagtgcc acgagctgaa
     2881 aggttcctcg gacgtccagc aatccagacg ttttgacaag caagggcctt actgaaccac
     2941 gcgcgccagc aagcggacct gtctcttctt cttctcttcc cagaccgtaa tcagcagggt
     3001 gctgagcagc accagcacgc cgccggcaat catgagcggc ctcattcgct ctccaagcca
     3061 gatggcggaa atggggacgc caaagaacgc aatcaggtaa ttggaaagag cggtctgaat
     3121 cgcgtcgaga tagtcgagcg ccttcagaaa gagaatcatc gaaagataat tatggaagac
     3181 agtcaggagg aagagtccta cccaggtccc ggcggtgaac tgcggtatac gcgcgaatat
     3241 atcggactcg gccgcaagcg tcagcggcgt catgatcagg aacatcgcca cgtaggtgta
     3301 gaacaacatc tccagtggtg aaaagcgctt cagcaccttc ttgccatacg aattgtagaa
     3361 ggcactgccg ttcacgccgg cgaagatcag aaggttgccg aagagataag ccgtgccgaa
     3421 attcagcgtt ccgtaatcga cgccggaaca cagcaacaca ccaccgaggg cgagggcgaa
     3481 agcgatccat cgtatgcgcg tcatccgctc gccgagaaaa agataggcca tgaacgccgt
     3541 gcagatggga agagccagcg tcagcatcgc ggcattactg gcgagcgaaa ggcgtgtgcc
     3601 ccaggtcacc agaaactgtc ccggaaaaac accggccgcg gcgagtagca aaaagatcag
     3661 caattctttc gggtgcggcc acgggcggcc cttgctgcgc aactcacggc ggacggacgg
     3721 atatagaaat accgtcgcca gcgtcatcgg tccccatacg gtgaaaaaaa aatccgacct
     3781 ggtcctggat gagtttgacg caggtgaatt gcaaagccca catcatattg cagaggaaca
     3841 aaaagagcca cgaccggccc gcgctcatgg cagcgccttg ttcccggccg caatcgataa
     3901 cgggttcgcc ggtagagcat agcgatcgag cagcgccaga atccggccgt tcaaaaaccg
     3961 gatatgccgt tcgtcttccg ggcgcagcaa aagacccgca tcgcgaacgg cggcgtggcg
     4021 gatgataccg cgatcgcgca gcagcagttt ttcggcatga tggaacagtt ccagattttg
     4081 caggctgaag acgatttgcg gtaacacgac ggcgaagatc tcataggctt cctcaacgtc
     4141 accggcgccg gcaagctcga acacccgcgc aagcagatcg gtcagcgcaa cacccggcat
     4201 gacggcggat atccctgcag gaatcaactc cagcatgtac atcccgcccc agccctcgat
     4261 gacctggacc gcgccgccgg attcctggcg gattcgagca gctttggcgg ccatcatggg
     4321 ctcctcgagc ttgatgtacc ggaattgcgg atggcgctca tgcagccgga cgatgaactg
     4381 cgggctcacc gaagctccgc ccgggttgaa gtcctgaatg atgacaggca cctgtgccgg
     4441 tgcgagcacg cggtcgaaat agcggaaaag gtcgtcttcc ggcaatgcga agagccgcgg
     4501 cacggcgacg gcgagtcccg ccacgccggc acgctgatgg gactccacag cctccacggc
     4561 ggacgccgcg gatggagtgt tcacctgccc gacgatagga agccggccgt tcgagtgcga
     4621 tacagcctca tacacaagct gcgaccgttc ctcatcggtg agcttgtaaa actcgctcgc
     4681 atacgccggc agacaagccg cgcaagcacc ggccgccgcg gcgaaatcga tcaatgcgcg
     4741 gaagtcctcc cacgcgatct ctccctccgg cgtaaacggc gtggggatca cgggaagaat
     4801 gccgcggatg ttcttcaggt catttcgcat tggcttatct caacgtttcg gacggaaatc
     4861 ctctatcggc ggcaggcttt cgccggtttt gaagctgctg ccgaattcct gaaagcgggc
     4921 ctgcaaagcg cgccagtatt catactccgc ctgccagcgg ccgagtgcgg attccaggcg
     4981 atagggccgg tactcggcga gccatgcctc cctgtaaggc tgcttgagtt cactgatgcc
     5041 gtccaacaga tcctggatca ggccgtgatt ctgcgacgca ctttggatgc gtatgagcag
     5101 atacacggcc tcgcggtccg gcttggatcc cagacgctca aaatacccgg ccatctccgc
     5161 cgcgtagaga tatttcatgc caaggtaatc caacatgcgc gctccaagca gcagaggacc
     5221 gatcgtagag ggctccccct tcaattccag agcgcgccgg aaatgggatt gcgcttcctc
     5281 cgcgttgagc cgtgcctggt gcagttcgcg gctgtgtgcc tgcgcacgct ctaatcgccg
     5341 gggcgcgagt ggatcggccc acaggctgtg aatcgtaccg ccgccgagcg ctttgccgaa
     5401 cgcctcctgc gactcggtga gcaactccag cccggtagcc acgtccgccg cggtgggccc
     5461 ggaatacacc agcgccgcat attcggaaaa gaaagaggaa ctgttcatcg gagcagcttg
     5521 ccagggtgcg gcggctccat aagccatgcc tggcagggac atgcgataga gtacctgacc
     5581 gctatcgctc cagcccgtat tcaacagccc aatcgctttc tctttcttgc ctgcctccag
     5641 gaatccgccg atgttggcaa acgaggtccg gaagctcggg gcaatttcgt tccagttcca
     5701 gacggcgggt gccaccacga cgtcgacgcc tgcttttcgc agcggctcca ccataggtgt
     5761 gtagtctttt tcgggtgaat aatgccatgg aacagcgatc gttcctggag gaagttgcga
     5821 aataagttcg ggatacttgg tgaaaatacg ggcgccggct accatgtccg cccaaaacat
     5881 catgcgcttg ccattcttcc gaacgatatc agagacggtc ttcaactgct caatataaag
     5941 ctggccggga ggcactccgg cggccttgct gccgagttgt tcgagggccc acggctcatc
     6001 gagaccaaca tgaaaccact cgctggggaa tagtgctgcg agttggccga tccagtcctc
     6061 gagcaccttg cggactcccg ggtgatgggg attgaattcc ccgccatagg gaaccggagc
     6121 catttgcgca taacgttcca cccgaaagag gtcatgcaaa tgtccataga actcgaggca
     6181 cggcacaacg tccacgtggc gctctttcgc atacgcaatg atgcgccgca gttcttcttt
     6241 ggtgtagcgc gcgtccgggt tgacgagcgg ataaccatcg atttcgatgc tcgcttccga
     6301 atagaagtaa tactggttcg ctttccaccg ggacagaaaa tcgatctgcc gcttgatttc
     6361 ggcttcggtc ggcatcggcc cgtggctgat atccatcatg aagccgcgat agggtaacgc
     6421 cggccagtcg cgaatctcca cttcgggcag cgcgccaccg gtggcaagct gccgcatcgt
     6481 ttgaacgccg taaaacagcc ccgccgtgct tccggcggtg acctcggctc caccgggagt
     6541 aactttgatc gaataccctt ccggcgaatt accaacctgc tgcggcgtcc gccggagaat
     6601 aatctccgga ccggagcctt tgccccggtc cagagaaaaa gccgacgtga gtacggacgc
     6661 agcgaactga tcctcggcac tcgccggtgg gaccacgcga acgctgacgc cgctcaatga
     6721 aaggctgccc accccgtagc gaacctcccg gggctgcggc agcaacgggc gatgttccgc
     6781 tgcgccagcg gccagcggca gcaacaacag cgcaatggga aggcgcattg tcagaagtcg
     6841 atgcggccgc gaagctcgat gcggcgggaa cgtccgccgt ctcttacgtc gctgatgata
     6901 cccacgctgc ccggagccga aatgttcaag gcggggcctg cgaaattcgc gtggttgaat
     6961 gcgttcgaag ccgctccggt gagagtgaac ttgaaacgct ccgtgaggtc gaacgttttg
     7021 gctagtgaga tgttatgcat gtggagcccg gggccttcca gaacataggc gccggaattg
     7081 ccgaagcgtc cggtgggtgg aacggcgaag caactggcat cgaaccagcg atcgatgctg
     7141 cgctcacccg ccggcagatt tccgtcgcag atgcggtccg gaagtcctcc aacggtattg
     7201 gtattcgatg gatcgcggcc ggagaaactc ggcgagaaga attggcccgt ctcgaagtag
     7261 gcgatccagt aaagctgcca ccctccgaga atatgctccg ctacagccgg cgcaccggcg
     7321 agataccggc gtcccttgcc gaccggaagc tcccagacag tgttcactac cacacggtgg
     7381 cgcgcggtga aaggatcgcg gctccacaca ggctcggcat aaggatcctg caaaaccgtc
     7441 gtggtgttgt aattggatga ccaggtccag tgcgcattca acgtcagcat gccgactttc
     7501 cgctgcacct cggctagcat tgcattgtaa ttggccttac catccgagcg ggcgtaagtg
     7561 gtgccaacga actgcgacca gggcctccgg gccgcggtga acggaatgag gcttgcctcg
     7621 ggcttgttga tgctgatgtt gtagttcatg ccgcggttgc gcgagccgat ataggcaagc
     7681 ttcaatccca cttcgcctac ctgccgctcg acggtcaggt tgaactgatg aatcttgccg
     7741 ttgtcggtat ccctgggaaa accggtgaca ctttgcgatg ggatgttggc gcccgccaga
     7801 gaggagggga aaggattcgg gaactgcaac agaggctggc cgtttatgat ctggttgttg
     7861 taggtttcgg tgatctcgaa aggcccgccg gtcaccgctc gcgcgtaacg gccgatggtt
     7921 tcattgaaaa tcccgtaacc gccgcgcagg acggttttgt cgctcaggcg gtaggccgct
     7981 ccgatgcggg gcacaaaatt cgtattactt ggattggggc gcggattgcc cgccgccagc
     8041 gtaatggtcg ccggatacag cggactgacc ttgtccatcg cattctgcga gatgatcacg
     8101 ttgcccgtcc cggggtccca attgtaaacc aggccgtcat cgtaggtggc ggaaccgaaa
     8161 cgatcccacc gcaggccgat gtcgagagtg aggcgattac tcaccttcca ggagtccgtg
     8221 atgaagatcc ccagttcgct atccgtcagc ttgcggttgg tgagagggtc gaggcgcgaa
     8281 ctgctgaacg gaaggcccag caggaagtcg gcgtagccat aaccggtgag cgatccgttg
     8341 aagttgaacg agccgtaatt gccctccggc acgttaccgc ggaaccggtt ttgcggcttg
     8401 tactcaccgc cgaacctcac tacatgccgg cctgtgttcc atgtcgccgt atcggcgaag
     8461 ccccagttgt gatcgttctg tacctcgccg ccgggctgcg tccgcagcgt cggatatccg
     8521 gcgatgtcaa ttctggggaa tccctgcgcg ctcagaccga gacgattgac gccttgcagc
     8581 ccgagttcct ggaccgcctc gtcacccttg aaaggcgtaa cgccgtagag ttcgtcgcca
     8641 tcggtgatct tttctttgta gaagccgacc cgggccgtat tcaccagcgt cggcgagaac
     8701 acgtgcgtgt cttcgatgac ggtgtgaacg ttgaaccgta cgcgagtcca ggcaaactgc
     8761 gggaaattcg tcgggagaac atacaggccc cagttttcga tcacgcgccc catgagccgg
     8821 ttgctgctgc tcaggtggta gtcgacccgt tgagtaaagt ctttgcggag agcgtagtcc
     8881 gtagggaagg ggaacgtgaa ggcgtagttg ctggcgagag cgtccgatcc cagccggttg
     8941 ggagcaggca aatagctttc attcacccgc gccgagacgg aactgatccg gttcgccggg
     9001 atgacatttc cgggaaacgg atcgccggta agggggtccc tgatgatgac cggcgacgag
     9061 agcccaagca attgcgaaaa gtctccctgg cgcattctgt cggtgggaac atccctgaga
     9121 tagaactgct tactgggaac cttgaggatg ttggcggagg cgtaaaagaa cgccttatcc
     9181 cgcttgatgg gccccgcaag gctgacgctc gtggtgtgga tgagcgtttt cgccttctgg
     9241 ctttcgaaga attcccgtgc gttgaaagcc gaattctggt ggaaatacgc cgcccgccca
     9301 tgccactcgt tggtgcccga tttcgtgatc atgttcatat agccaacacg tgcgaattcg
     9361 gcgctattat ttaccgtcac cacggtgatt tcctgtgtgt ccaggatgtc gttgatctga
     9421 tttacggcat tgtcgttcgt gtgcccgtcc tgtccttcct gcacctggct gctcgactgg
     9481 ccggcccact gcgaactcca aactccattc gtctgtgaca ccagcggcag cactgtgatg
     9541 aagaggcttg ggtcgagcgt cgcttccgca ccgacccacg ggttgtcgaa atgtctcgat
     9601 gtgttcacag aaccgccaag tttagcggta tccgtttcga taacggcagc atcggcacga
     9661 accatgatct gggcgtctac ggcaccaatt tccaatgtga cgttgatacg ccggacctgg
     9721 ttggcgtcca gaatgatatt ctgcgcgacg aaagccttga acccgcttgc cttcgccgtc
     9781 aattcatagg ttccgcgctg aagatcggga aattcaaaat ccccgttctc attcgtcttg
     9841 gcaacacgcg tcgcattggt ctgagtgttg gtgactgtga tctcggcgtt tgcgaccagc
     9901 gcgccgctgg gatccacggt cgtgccgcgt atcgtggata gcgcaagttg cgcgatcatg
     9961 ggcaacgata gcaggatcgc cgccagcaac aaaaacagaa ttttcctcat agtagctccc
    10021 tcaccagtaa cagttgttaa acatatcaag ggcctgcgtg accccaatgc ataagggaga
    10081 gtattcccct ctgcgatcct acggtcaagc gcaaggttcg tttctaacgg ga
//
LOCUS       DASTMK010000417        17566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_117213, whole genome shotgun sequence.
ACCESSION   DASTMK010000417 DASTMK010000000
VERSION     DASTMK010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17566
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117213"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VFQ79_17910"
     CDS             <1..1446
                     /locus_tag="VFQ79_17910"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141604.1"
                     /translation="EFERTRPSSATEAFFQSGPERDYIVAHRSVSGNYRVLRHEYVHL
                     ALSHSSGRLPLWFEEGTAEFYSTIDISGTRLRIGGPVPGHIRKLQSAGLLEAGVFAAV
                     DRDSAWYLDRDKAGLFYAQCWALVHMMNLAPGYREQMPVFADKLAAGDPPAQAFHEAF
                     GNDLAAALADVVRYLRRPFRVAHVAWEPSQHEIQAQVERIDGTEAALAIGELLIYAGK
                     PKLAEEIYARVEKGGTDSPESVAGSAMIALRRGSAGEAMRQFERAIALGSRQASVFFE
                     YAMLLRDSGAEAERYRPLLEQAIAIQPSLAEAQFILGLEYSREGNEEKAVEHYRKAAE
                     ILPRQANFWHALALSYHKLGQRELALRCARRTLQAAANPAEREMAEAALKLTSNQQAA
                     PEKRSGVATPDSWQNSPGDSAIDGQLVRVDCEESEARLHLKTSAGDFVLKATRNTRGD
                     IRQLECGVQEPLNVSVGYDAATKEIRSIVFR"
     gene            1627..2187
                     /locus_tag="VFQ79_17915"
     CDS             1627..2187
                     /locus_tag="VFQ79_17915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0141605.1"
                     /translation="MTRVEASALVASLFENWYSSLVRYVARTTQDPQGAEDIVQESFL
                     RLYRELRSGKEIRNPRAWTLCVARHETGRVLRERCHDESNHDGTLDLDEIPDRLPRSP
                     DREIELDELSKLLQVLTRREEEVVLLRMASMTYREIAGRLGISSSAVNTLLARALRKL
                     QDAVQVKVEGRTGSRHYAKQIPSTLQ"
     gene            2363..4165
                     /locus_tag="VFQ79_17920"
     CDS             2363..4165
                     /locus_tag="VFQ79_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141606.1"
                     /translation="MDARDRFFANVELEERVSSSTPSFLISESRRRRASRVFAAVAAC
                     GILAYFAYSPSPEALDPQNLLHAAQAREASQFSLPMSFHQEFAVEIEQREPKGPERSS
                     RLKVWREGGGQRFAARWEGDSGDLKYAAWQPAPGEEREFEPSEGSGIVVRRHRSASQP
                     LLRILRSGGGGVQEIERAFFRWLRQREWTLVSLSSEFALFAAEEGVVLHAERLSSAEG
                     NRIRLTAERDEDDLKLELVLELEPESLRPRLQVARISSAGRQFELRLASRRSVAGATA
                     INASLFRPQSPALPEVLNRPGDLTGDSVLNSPSITPSQLDLETTEVEILYALHRAGLC
                     LGEPVEVVRLDGKGLRVDAVAETADRRNAVLAALKEVSESPFVEVRVRTTAEEPDLPL
                     RTGELPATRFTTRDLPIEELLERRLGGQEGGPDLHAEVVRLSHSAMLLAEGAFAEAWA
                     IRDISVRFPERSVQQLPPQTRWLLEVMLRDHVSALSRQVREARSSLNTILAAAPPHSN
                     KVQPVDGSAVQPEDYLVLFEVVKHLHSLLSAAFAGNDTPAGVSAGELPGLIGVSLDTV
                     EAQAQKLTGHLAWRPPEPAEDVRAGDESERNRTQ"
     gene            4162..7572
                     /locus_tag="VFQ79_17925"
     CDS             4162..7572
                     /locus_tag="VFQ79_17925"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0141607.1"
                     /translation="MKKSTPSRPGSIELFVLAATVFLIAGASPLCGQTAELTGRVMDS
                     SDAVIVDADVTAINVDTGVRRPTTSNELGLYRVRLLPPGNYRVMVQKPGFRPINQSGI
                     TLTVDQVARIDFVLQVGPVTETVEVAVSAPLLESESANYGQVVTQRSVEELPLNGRDP
                     MFLITLTPGVSTGSSFDASQAGNNNSGRNWFKTDFSVGGGRNFEQEVLLDGAPNTSSD
                     RNYAIYIPPVDSTQEFKVLANPYSAEYGRTAGAVVSMVTKAGTNEFHGLAYEFHRNSV
                     LDANLFFNNRNNVRRGSFRRHQFGANFSGPIFKDRTFFFADYEGFRQAVPATFLTTVP
                     TELQRRGDFSQTFTRDGRLIGIYDYATLLRGSDGRWTRQPFPGNVIPQDRIDPVAAAV
                     TSFYPLPNLPGDPLTAANNYINAAKDVLDMNKYGIRVDHHIGTGNRVFGRYSAQKSNN
                     RRPGRWDAGEAPSNIQQIDDFLNIVVGDSHTFSPTMLGEIRASVARGYPVQSVASAGF
                     NLSALGFPAPFLSVAESFFPSFVPSDVSQIGNGFINLQPRDTYSLVGSVSKMAGRHTF
                     KFGGETRTLRFHQYQVEDPAGRFTFGRLFTQGPDPLVARTDAGFGFASMLLGAGTSGK
                     IDHRAGLSLQRLYHAFFVQHDWKASSRLTVNLGLRYDVTTGTNERYNRLAWLDLDSPS
                     PFGSVVGLPLRGILQYTGTDGNRRNQLATDGNNFGPRVGIAFQVDPSTVIRTGYGIFY
                     VAMTTRNDGSIGFNTESPWVATLDDLRPEYLLRNPFPNGFNFPVDDRSPTTNVGFGLD
                     GYLGYEPVGYTQQWSFSVQRQVASGILVDAGYWGNKGTKLAYGAGFQENFLDPAYLSL
                     GAALNERVANPFFGHIATGALAASTVTRRQLLLPYPQYTSVIRTSPSAASAIYHAFTL
                     KAEKRATHDLSFVVSYTLSKIIGDSSGRLGSANVGGIQNMADRRQERHILSIDVPQRL
                     VAGFVYDLPFGRGKWIGSGAPRIANAMIGGWRLSGISTFQSGFPVSIARPSVNSGRSA
                     KLDDPSIDRWFDTTAFSPALPFTFGNVGPSLPDVRRHGVRNFDFTLGKDFRFLERFRL
                     QLRSEFFNALNRPQFGPPNGSVTNPAFGQVTTQANSPREIQLGLKLYW"
     gene            7578..8762
                     /locus_tag="VFQ79_17930"
     CDS             7578..8762
                     /locus_tag="VFQ79_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141608.1"
                     /translation="MKQKIIVVFGSLAVLLGGVAWMTRPAPPEEEVDVLSGWRKPLTV
                     RPVAPSQDRHTIHAYFNTCPESPDGRWVVYYSSGAPDSHQGEIRILQRATGEERTIAR
                     GLTVEDAHRAACQQWISGGRRVVFHDFRDGHWVVAAVDINTLEERVLARDRMVGWGQP
                     RSNLVPIYGPHYDRAAFKDLELLDVESGERRTVLKAAEVRKAYPELVASKYADRDFSI
                     YFPILSPDRGRVFFKLAAPSGGDFRSKTASSRHLIVVYDIAKPQFLFGHENWGHPAWH
                     PDSRHIINVGPVLIDSEAQSIEPVPGLPRFPGSHPSFSPDGALFTTDTRVGTEGRWGV
                     VVGDVRGGRHELVHTFDNSKGAASWRVSHPHPVFSADGRRVYFNVNATQWTQLHVAER
                     AD"
     gene            8762..10300
                     /locus_tag="VFQ79_17935"
     CDS             8762..10300
                     /locus_tag="VFQ79_17935"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0141609.1"
                     /translation="MVVEASSRRRFLGGASSLCALSLAEPAQQQAGQGEYDVLVWDST
                     PAGIATAIAASRAGLRAAIVTEDKHVGGMQTSGLGNTNAGQRETVGGLAREFHHRIHK
                     YYTNLYGANSANVQACQNGFYFEPHVAERVYLEWMREAGVACLSEEWIESVEKNGPRL
                     MAVRTARGNRIGARIFVDASYEGDLLKLTGCSYHLGREAASKYGEPLAGVRFPPERAG
                     QADRKLQAFDYRLCLTDVAGNRVPFAEPANYDPSDYFFQGARLKHAPPQRLHQALPLN
                     PMPNRKTDSRTGEWVGASWDYPEAGPAERKRIEDAHRNYSAGYIWFLLQDAPLPAPLR
                     EELSRWGLAKDEFTDNGNWPYHIYVREGRRLAGDFVITQQDVTERRFFEDGIALGNFY
                     LDVHPVDMLVTHDMVVAEGGLGNVRVKPYEIPYRSLIPKRSEAVNLLVPVCLSASHIA
                     FSTIRMEPVWMMLGHACGVAASLALESGASVQDVDGRLLRKRLAGDGQIVDARPFTRK
                     WPFR"
     gene            complement(10349..10903)
                     /locus_tag="VFQ79_17940"
     CDS             complement(10349..10903)
                     /locus_tag="VFQ79_17940"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0141610.1"
                     /translation="MPLGKRGPYEVVSSREVYRGARISLRCDHVIQPGGSERDFDILE
                     MVEGSTVLALSDDHTAYLTREYKYAVGRECLELISGAIDEGETALEAAQREIKEEAGL
                     TASEWIPLGVLDPFTSTVRSPNHMFLALGAVLGEPDPDEGEVIHTVAVPFSQAVEMVL
                     RGDITHGASCVAILKAERLLRERR"
     gene            complement(10903..11640)
                     /gene="bshB1"
                     /locus_tag="VFQ79_17945"
     CDS             complement(10903..11640)
                     /gene="bshB1"
                     /locus_tag="VFQ79_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEU0141611.1"
                     /translation="MIVSDLTEVPSVDVLAIAAHPDDVEQTCGGTLIRMAEMGYRTGV
                     LDLTAGDMGTRGSPELRLLEAGEAGERMLLSWRGNRRFPDARLENTISVRMTLAVDIR
                     RLRPRVVILPYWEARHPDHYRAGEIAYEACFLAGLRKLDEYSEPHRPSKVIYSSLYAN
                     VTPSFVVDITRQFDRRMDALLAYGSQYGTVEAGSELFPKEREIRERLAAVARFYGNLI
                     GVKYGEPFVVKETIRVDDILQMGVRSF"
     gene            complement(11640..12308)
                     /locus_tag="VFQ79_17950"
     CDS             complement(11640..12308)
                     /locus_tag="VFQ79_17950"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0141612.1"
                     /translation="MIPLRDTQPSYSTPFVTILLIVVNVFVFLYEVSLDDYTRNAFIS
                     TWGMIPARFEYTDMLTSMFLHGGWMHLIGNMWFLWIYGDNVEDTLGHWKYLLFYLLCG
                     LAAAGTHAVTNLDSRIPTVGASGAIAGVMGAYLIKFPHSRILTLVVVFVFITTVEIPA
                     VLILAYWFLIQLASGMWSVGYSQLSQGGVAWFAHVGGFVAGVILINVMRTRERYRRRR
                     DLHW"
     gene            complement(12309..13265)
                     /locus_tag="VFQ79_17955"
     CDS             complement(12309..13265)
                     /locus_tag="VFQ79_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0141613.1"
                     /translation="MAEFDVVGVGLNATDTLLLLPKFPAYAGKVAFEDEILSPGGQVA
                     SAMVTCASLGLRVKYIGTVGDDERGRIQLESLAGTGINIDHVQVRENCPNQTAYILID
                     RTTGERTVLWRREECLRLKPEEITPEQITCARLLHIDGHDTPAVERAAAIARSASIPV
                     TVDVDTIYHGFDRVLPLVDYLVTSSEFPTQWTNERDPFRALEMIQNEYGMKVAAMTLG
                     SLGALARSEGRFVYSPAFVVNCVDTTGAGDVFHGAFCYAVLKGMDMAQLLDFSNAMAA
                     LNCTALGARGGIRRLEDATALMSRAERRSHAEFEVRTVTSTR"
     gene            complement(13258..14427)
                     /locus_tag="VFQ79_17960"
     CDS             complement(13258..14427)
                     /locus_tag="VFQ79_17960"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEU0141614.1"
                     /translation="MTERIYYNDSYQTEFRAHVLESSEDGRRLYLDRTAFYPASGGQP
                     HDTGCIDNAMVTDVIEEEDGRIAHILSTPVEHSDVNCRIDWARRFDHMQQHSGQHLLS
                     AVLMELFSVETLSFHLGDAASTIDLSVASLEPEQLRKAEERVNQIVFENRPLAVEFRD
                     ASENLELRKPSERQGKLRIVTIEGLDRSACGGTHVRSTGEIGAVLIRRLEKMRGNLRI
                     EFLCGMRAVRRARLDYEALLEISRLLSASLDETPSLVAVQLRRLNESEKARNKLSIEL
                     ARIEGAALNEATEPGPDGVRRASRTIARGPIDDDLRALAQGFTSRNKAVFAAFVEEPP
                     SLLVAVSSATGLHAGNLLKSLLAGPGGRGGGNAQLAQGSVSSREALERIRGELNG"
     gene            complement(14440..14796)
                     /gene="ndhC"
                     /locus_tag="VFQ79_17965"
     CDS             complement(14440..14796)
                     /gene="ndhC"
                     /locus_tag="VFQ79_17965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU0141615.1"
                     /translation="MPEGYIPIFIFMAVALAFPIVALGLAKLVRPAAPSVTKLEAYEC
                     GIQASSGARGRYTVRFYIIAILFVIFDVEAVFLFPWAVRYRQLGWFGVAEVAVFLGIL
                     IVGYIWAYKKKALEWV"
     gene            complement(14830..16830)
                     /locus_tag="VFQ79_17970"
     CDS             complement(14830..16830)
                     /locus_tag="VFQ79_17970"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0141616.1"
                     /translation="MIRILVLFLGALAFAQTDNTALRSMEESKTAVTAYLERCARRIT
                     DRAASEIRSREEWERVRARRLEEMRDILGLLPWPERTPLNVKITNTLDKGSYTIENIA
                     FESIPKFYVTANLYLPKNRPGKAPAVIYVCGHAMEPHGSKAAYQRHGISLAKNGYAAM
                     IIDPIQIAEAFGVHHGIHSQEMYDWYSRGYTPAGVEVWNAMRAIDYLETRPEIDADRI
                     GMTGRSGGAAMTWFTAAVDPRVKVAAPVMGISTYAANVQLNTQRGHCDCMFTINALLH
                     DMMHQGSLIAPRPLLMAHGKLDSLFPVPGYEEFEAKVGGLYSSYGERTGFRNIVVETG
                     HQDSDFLREEVIRWFDRYLMRIPERKLDMDYSNAAPESLAVFPEGSPADAQNYRIQDT
                     FLPTPRLKRYANRASWEAHRAGVVETLRRKVFAAFPPTAVDLKVRRPLSESPPRELEL
                     DFESEEGITVRAVYRRHEKPGKHPALLYVASDGEDARSINAVFRSIAPRMIVYPRGVG
                     EIPWPKNFWKDTLRNAMHVGHTVDSMRLWDVMRAVQVLRLQDDVDPARIMVVGQGVSG
                     ALGLYAAVLDPQIHQVTLIDPPVTHVQGPIFLNVLRYLDLPEAAAMIAPRHLNFYARI
                     PKEFESARQVFELYGKPENLFLTMNFDKILEGRYDHRFASGL"
     gene            16851..>17566
                     /locus_tag="VFQ79_17975"
     CDS             16851..>17566
                     /locus_tag="VFQ79_17975"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEU0141617.1"
                     /translation="MGLITVSGEPGCRFEEAGRLTAQRLRCEFVTESSLAGMLIEEFG
                     PQIAMPDKAYPHVVSSIVARIATQHHVVVCLLGAETLFRHYPGVLRVHVDAPEPRRTG
                     NLMLDGRLTRSAARKQLRERQVKQRAERKRKFGRATTPAHLFDLVLNADGLEAEEMAE
                     IMETAARVKGVFDHGLLGASAEAQLQFQVRLELAKHGITPIGHAELKRRAFGHPSEEV
                     FANVLDFYRVAWKYEPKSFP"
BASE COUNT         3491 a         5182 c         5333 g         3560 t
ORIGIN      
        1 gagttcgagc gcacgcgccc ttcttccgcg acggaagcct tctttcaaag cggtcctgag
       61 agggactaca ttgtggcgca ccggagcgta tcgggaaatt accgggtgct gcgccatgag
      121 tatgtgcatc tcgccctcag ccattcctcg ggacggcttc cgctatggtt cgaagaaggc
      181 accgcggagt tctattccac aatcgatatc tccggaacgc gcctccgcat cgggggaccg
      241 gttccgggtc acatccgcaa gctacagtcc gcggggctgc tcgaagcggg cgtgttcgcg
      301 gccgtggatc gcgattccgc gtggtattta gaccgcgaca aggcgggcct gttctacgca
      361 cagtgctggg cactggtgca catgatgaat ctcgcgcccg gctaccggga gcagatgccg
      421 gtgtttgccg acaaactcgc agcaggcgac ccacccgccc aggcttttca tgaggcattc
      481 ggaaacgatc tggcggccgc cctggcggac gtggtccgct atctgcgaag gccgttccgc
      541 gtggcgcacg tagcatggga accgtctcaa cacgagattc aggcgcaagt ggaaagaatc
      601 gacggaacgg aagcggcgct ggcaatcgga gaactgctga tctacgccgg aaagccgaag
      661 ctggcggaag aaatctacgc acgagtggaa aagggcggca cagactcccc cgaatccgtt
      721 gccggcagcg ccatgatcgc cctgcggcgc ggcagcgccg gggaagccat gcggcagttc
      781 gagcgcgcca ttgcgctcgg cagccggcag gcttccgttt tcttcgaata tgcgatgctg
      841 ctgcgtgatt ccggggcgga ggcggaacgg taccggccct tgttggaaca ggccattgcc
      901 atccaaccgt ctctggccga agcgcagttt atcctcggcc tggaatactc gagagagggc
      961 aacgaagaga aggcagtgga gcattatcgc aaggcggcgg aaatactgcc gcggcaggcg
     1021 aacttctggc atgctcttgc actgagttat cacaagctcg ggcagcggga actggccttg
     1081 cgatgcgcgc gccggacttt acaggccgct gcgaacccgg cggaaaggga gatggcggaa
     1141 gcagccctaa agctcacctc caaccaacag gcggcgcccg aaaagcggag cggtgtggcg
     1201 acacccgata gctggcagaa ctcccccggt gattcggcca tcgacgggca actggtgcgc
     1261 gtcgattgtg aagagtcaga agcgcgcctg cacttgaaaa cttccgccgg cgactttgtc
     1321 ttgaaagcga cacggaacac caggggcgac atccgccaac tcgaatgcgg ggtgcaggaa
     1381 ccactcaacg tgagcgtggg gtatgacgcc gcgacgaaag agatccgcag tatcgtgttc
     1441 cgataggcgt taaccccggg ctagcatcgg aaagtcctgc ccggagagcc gaagtcattt
     1501 ttcccgcact ggtttgtggt gaattcacgg cgggcgagac tcattaacaa tatggggttc
     1561 tttaaccata gatggcggca cgatcttgcc ggatctctgc gcgtggaata agaaactgaa
     1621 attgagatga ctcgggtcga agcgtcggca ctggttgcca gcctctttga aaactggtac
     1681 tcgtctctcg tccgttacgt tgcgcggacg acgcaggacc ctcaaggagc ggaggacatc
     1741 gttcaggagt cgttccttcg cttgtatcgc gaactgcgat ctggcaagga gattcgaaat
     1801 ccccgggcgt ggacgctgtg cgtggcccgc cacgagaccg gccgggtttt acgtgaacgg
     1861 tgccatgacg agtcgaatca cgacggcacc ctggacttgg atgagatccc ggaccgtctc
     1921 ccgaggtcac cggaccggga gatcgaattg gatgagctga gcaaactcct gcaggttctt
     1981 acgcgccggg aagaggaggt cgtgctgctg cgtatggcct cgatgaccta ccgggaaatc
     2041 gcgggaaggc ttggcatcag ctcaagcgcc gttaacacac tgctcgcgcg ggctctccgc
     2101 aaactgcagg acgcagtgca ggtgaaggtg gaagggagga caggctcaag gcactatgca
     2161 aagcaaattc catcgacact gcagtgacga gcgccttctc gcgtacctgg atggtgagct
     2221 atcgcttccg gccgcagggc gcgtgcgccg ccaccttgac tcttgctggc actgccggaa
     2281 tcgtcttgga actctggctg aacaggcacg cgctatagcg gaggcgtttg aacggcaaaa
     2341 ctatcccagt gccaggcacg tcatggacgc ccgggatcgc ttcttcgcca acgtggagtt
     2401 ggaggaacgg gttagttctt ccacgccgtc tttcctgatc agcgaatccc gccgccgcag
     2461 agcgtcgcgt gtgttcgcgg ccgtggcagc ctgcggaatc cttgcctact tcgcctactc
     2521 gccatctccg gaagccctcg atccgcagaa tctcctccac gccgctcagg cgcgtgaagc
     2581 ttcccagttc agtttgccga tgtcttttca tcaggaattt gcggtcgaga tcgaacagag
     2641 ggaaccaaag ggaccggagc gatccagccg cttgaaagtt tggcgggaag gtggtggaca
     2701 gcgcttcgcg gcccggtggg aaggggatag tggcgatctg aagtacgctg cctggcagcc
     2761 ggcgcccggg gaggagcgcg aattcgagcc ttcggagggc tccggcattg tcgtccggcg
     2821 ccatcgtagc gcatctcagc cgttgttgcg catcctgcgc tctggcggcg gtggtgtcca
     2881 agagatcgag cgcgcgttct ttcggtggct acgccaaaga gaatggacgc tggtttctct
     2941 ctcctcggag ttcgcattgt ttgctgccga ggaaggagtc gttcttcacg ccgagcggct
     3001 ttcttccgcg gaaggcaacc gtatcaggct gactgctgag cgagacgaag acgatctgaa
     3061 actggaactg gtccttgagc ttgaaccgga atctctccgt ccccggctcc aggttgcgcg
     3121 tatcagttcg gcgggccggc agttcgagct tcgactggcg tcgaggcgat ctgtcgcggg
     3181 cgcgacagcc atcaacgctt cgctcttccg cccgcaatcg ccggccctcc ccgaggttct
     3241 gaaccggcca ggcgatctga cgggtgactc cgtgctcaat tctccttcca tcactccatc
     3301 gcaactggat ctggagacga cggaagtgga gatcctctac gctctccacc gcgcaggcct
     3361 gtgcctgggt gagccggtgg aggttgtgcg cctggacggt aaagggcttc gggtggatgc
     3421 ggtcgcggag acggcggacc ggcgcaacgc ggtgctggct gcgctgaagg aggtatcgga
     3481 atcgccgttt gtcgaggttc gtgtcagaac aactgccgaa gagccagacc ttcctctgcg
     3541 gaccggagag cttccggcca cccggtttac gaccagagac ctgccgattg aggagcttct
     3601 tgagcgccgt ctgggcgggc aggaaggcgg gccggacctg cacgctgaag tcgtgcgtct
     3661 gtcgcactcg gcgatgttgc ttgctgaagg cgctttcgcg gaagcatggg caatccggga
     3721 catctccgtt cgcttcccgg agcgatccgt tcagcagttg cctcctcaga cgcgctggct
     3781 gctggaagta atgttgcggg accatgtctc cgctctatcg cggcaagttc gcgaagcccg
     3841 atcgtcgttg aacacgattc tggcggcagc tccgccgcac agcaataagg ttcagcccgt
     3901 tgacggtagt gccgtccagc cggaagatta tctggttctg tttgaagtcg taaagcatct
     3961 ccacagcctc ctcagcgccg catttgcggg caatgatacg ccggctggcg tctcggccgg
     4021 cgaattacca ggtcttattg gagtcagcct cgataccgtc gaggctcaag cgcagaaatt
     4081 gacgggacat ttggcttggc gccctcccga gccggcggaa gatgtgcggg ccggtgacga
     4141 atcagaaagg aatcgaactc aatgaagaaa agtactccgt cccggccggg ctcgatagag
     4201 ctttttgttc tggccgccac tgtctttctc atcgcaggcg caagcccatt atgcggtcaa
     4261 acggccgaat tgacgggccg tgtcatggac tcctccgacg cggtcatcgt ggacgccgac
     4321 gttaccgcaa tcaacgtcga taccggagtg cggcggccaa ctacatctaa tgaacttggg
     4381 ctttaccggg ttcgattgct gccgcctggg aactatcgcg ttatggtcca aaagccaggg
     4441 ttcaggccca tcaaccaatc gggcattacg ctgacggtgg atcaggttgc gcggatcgac
     4501 tttgtgcttc aggtggggcc ggtgaccgag acggtagagg ttgcggtaag cgcgcccttg
     4561 cttgagtcgg aaagcgccaa ctacggtcaa gtcgtgaccc agcgaagcgt tgaagaactt
     4621 cctctaaacg gccgcgaccc gatgtttctc atcactctga cacctggtgt ttctactggg
     4681 agctcgtttg atgcgtcgca agccggcaac aacaacagcg gtcgaaactg gttcaaaacc
     4741 gatttcagcg tcggtggcgg acgaaacttt gaacaggagg tgctcctcga tggcgcgcca
     4801 aacacatcgt ccgaccgaaa ctacgcaata tacataccac cggtagactc cacgcaggaa
     4861 tttaaggtgc ttgccaaccc gtactccgcc gagtatggca ggactgcggg cgcggtcgtg
     4921 agtatggtga ccaaagcggg caccaacgaa ttccacggac tggcgtacga gttccaccgc
     4981 aacagcgtgc tggacgcgaa cctgttcttc aacaaccgta acaacgtccg tcgcggctcg
     5041 ttccggcggc atcaatttgg cgccaatttc agcgggccaa ttttcaagga caggacattc
     5101 ttttttgccg actatgaagg ctttcgccag gcggtgcccg cgacatttct gacgacggtt
     5161 ccgaccgaat tgcagcgccg tggcgacttt tcccagactt ttacgcgcga cggcaggctg
     5221 ataggtatct atgattacgc aactctgctc cgcggctcag atgggcgctg gacgcgacag
     5281 ccgtttccgg gaaacgtgat tcctcaggac cgtatcgatc cggtggcagc ggccgtcact
     5341 tcgttttatc cactgccaaa cctgccgggc gatccactga ccgccgcgaa caactacatc
     5401 aacgcggcga aggatgtcct cgacatgaac aagtatggaa ttcgcgtgga tcaccacatc
     5461 ggcaccggca atcgcgtgtt cggtcgctat tcggctcaga aaagcaataa ccggcggccg
     5521 ggccggtggg atgccggcga ggcgccaagc aacatccagc agatcgacga ctttctcaac
     5581 atcgttgttg gcgattcgca cacgttctcc ccaacgatgt tgggcgagat ccgcgcttcg
     5641 gtcgcgcgag gatatcctgt acaatcggtg gcatccgccg gcttcaatct cagcgcgctg
     5701 ggtttcccag cgccgttcct ctcggtggcc gaaagcttct tcccaagctt tgtgccaagc
     5761 gacgtatctc aaatcggcaa tggcttcata aacctgcagc cacgcgatac ttactctctg
     5821 gtcggaagcg tcagcaagat ggcgggccgt cacactttca agtttggcgg tgaaacgcgc
     5881 acactccgct tccatcagta tcaggttgaa gatccggccg gccgcttcac tttcggccgc
     5941 ctcttcacgc agggaccgga tccgctggta gcccgcacgg atgccgggtt cggcttcgcc
     6001 agcatgctgt tgggagccgg aacgagcggg aagatcgatc atcgcgccgg tctttccctc
     6061 cagcgcctgt accatgcgtt cttcgtccaa cacgattgga aggccagcag ccggcttacg
     6121 gtcaaccttg ggcttcgtta tgacgtcacg acgggcacca acgaacgcta caaccggctg
     6181 gcttggctcg atctggactc gcccagccca ttcggcagcg ttgtcgggct tccccttcgc
     6241 ggtattcttc aatacaccgg cacggacggg aatcgccgca accagctcgc aacggacggt
     6301 aacaatttcg gcccgcgcgt gggcattgcc ttccaggtgg atccgtcgac cgtgattcgg
     6361 accggatacg gcatcttcta tgtggccatg acgacaagaa acgacggttc gatcgggttc
     6421 aacacggagt cgccctgggt ggcgacgctc gacgacctgc ggccggagta tctgttgcgc
     6481 aatccctttc ccaacggctt caattttccg gtcgacgatc gctctccaac aaccaatgtg
     6541 ggctttggac tcgacggata cctgggctat gagccggttg gatacacgca gcaatggagc
     6601 ttttctgtgc agcgtcaggt tgcgtccggt attctcgtgg atgccggtta ttggggtaat
     6661 aagggaacga aattggctta cggcgcaggg tttcaggaga attttctcga ccctgcctac
     6721 ctttcgctcg gcgccgccct taacgagcgc gtggccaacc ccttctttgg acatatcgca
     6781 accggggcat tggcggccag caccgtcacg cgccggcaac tgctgcttcc gtatccacag
     6841 tacacatcgg ttattcgaac gagtcccagc gcggccagtg ctatctacca tgctttcact
     6901 ctgaaggcgg agaagcgcgc cactcacgac ctcagttttg tggtttcgta tacactctcg
     6961 aagatcatcg gcgacagttc gggccgcctt gggagcgcga acgttggcgg aatccagaac
     7021 atggcagatc gccgccagga acgccacatc ctgagtattg acgttcccca gcgtcttgtc
     7081 gccggttttg tgtacgatct gccattcggt cgcggcaagt ggattggatc tggcgcaccc
     7141 cgaatcgcga atgccatgat cggcggctgg cgtctcagcg gcatcagcac ttttcaaagt
     7201 ggttttcctg tcagcatcgc ccggccctct gtgaacagcg ggcgcagtgc gaaacttgac
     7261 gatccgtcga ttgaccggtg gttcgacacg acagcgttta gtccggccct accgttcacg
     7321 ttcggcaatg tcggtcccag tcttcccgat gtccgccgac acggggtccg caattttgac
     7381 tttacgttgg gcaaggactt tcgcttcctg gagcggtttc gcctgcagct tcgttccgag
     7441 ttcttcaatg cccttaacag gccccagttt ggacctccaa acggaagcgt cacaaacccg
     7501 gcattcggcc aggtgacgac ccaggcgaac tctcctcgtg aaattcagtt gggcctgaag
     7561 ttgtactggt gagtggtatg aaacagaaaa tcatagtcgt cttcggttcg cttgcggtcc
     7621 tcctcggtgg cgttgcctgg atgacacggc cggcgcctcc cgaagaggag gtagatgttt
     7681 tgtctgggtg gcggaagccg ctaacggtgc ggcctgtggc tcccagtcag gaccggcaca
     7741 ccattcatgc atacttcaat acatgtcctg agagccccga cggacgctgg gtggtctact
     7801 actcgtcggg cgcgccggac agccaccagg gtgagatccg aattcttcag cgtgccaccg
     7861 gcgaagaacg gacaattgcc cggggcttga ccgttgagga cgcacatcgc gcggcgtgcc
     7921 agcagtggat ctcggggggg cggcgagtcg tgtttcacga cttccgggat ggacactggg
     7981 tagttgccgc agttgatatc aacaccctcg aggagcgagt ccttgctcgg gaccggatgg
     8041 tgggttgggg tcagccgcgg agcaacctcg ttccaatata cggccctcat tatgatcggg
     8101 ctgcattcaa ggatctggag ttgctcgatg tagagagcgg ggagcgccgg accgtcctta
     8161 aagcggctga ggttcgcaag gcttatcccg aattggtggc ttccaaatat gcagatcgcg
     8221 atttctcaat ttacttcccg attctcagtc cagaccgagg ccgggtcttc ttcaaactgg
     8281 ctgcgccaag cggtggcgac ttccgtagca agacggcgag cagccgccat ttgatcgttg
     8341 tttacgatat cgcgaagccg caatttctgt ttgggcatga gaactggggg cacccggcct
     8401 ggcatccgga ttcgcgccat attataaacg ttggcccggt gttgatcgac agcgaagcac
     8461 aatcgatcga accggttcca ggactaccgc gcttccccgg gagtcatcca tcgttcagtc
     8521 ctgacggagc gctattcacc accgatactc gtgtcgggac cgaggggcga tggggcgtcg
     8581 tcgttggaga cgttcgcggc ggacgccacg aactcgtcca cacgttcgac aactcaaaag
     8641 gcgcggcgtc atggcgggtt tcccatccac atccggtttt tagcgcagac ggccggcgcg
     8701 tctacttcaa tgtgaatgcc actcagtgga cacaactcca cgtggcggag cgcgccgact
     8761 gatggtggtt gaggcttcct cgcgccggag atttcttggg ggagcatcct cgctctgtgc
     8821 gctgtcattg gcggaaccag cccagcagca ggcagggcag ggcgagtacg atgttctggt
     8881 ctgggactcg acaccggcgg gtatcgcgac ggctattgca gcctcgcgcg caggtctccg
     8941 agccgctatc gttaccgagg ataagcatgt cggtggcatg caaacaagcg gcctcggcaa
     9001 tactaacgca gggcagcgcg aaaccgttgg cggcttggcg cgcgagtttc atcaccggat
     9061 tcacaagtac tatacgaact tgtacggtgc gaactcggcg aatgtgcaag cgtgccagaa
     9121 cggtttctac ttcgaacctc atgttgccga gcgagtttat ctggagtgga tgagggaagc
     9181 gggcgtcgcg tgcttgtctg aggaatggat tgagtccgtc gagaagaacg gaccacggtt
     9241 gatggcagta cgaactgccc ggggcaatag aataggcgcg cgtatctttg tggatgccag
     9301 ctacgaaggg gacctcctaa agctaaccgg ttgttcctat catcttggcc gcgaggcggc
     9361 ctcgaagtat ggagagccgt tggctggagt tcgatttccc ccggaacgag ccggacaggc
     9421 cgatcgcaaa cttcaggcat tcgactatcg tctgtgtttg accgatgttg ccggcaaccg
     9481 ggtcccgttt gccgaaccag ccaattacga cccatcagac tactttttcc aaggggcccg
     9541 gctcaaacac gcacccccgc agcggctgca ccaggcgctg ccgctgaacc cgatgccgaa
     9601 ccggaagacc gattcgcgga ccggtgaatg ggttggtgct tcctgggatt acccggaggc
     9661 cggaccggcg gaacggaagc ggattgagga cgctcaccgc aactacagcg ctgggtatat
     9721 ctggtttctt ctgcaggatg caccgctgcc ggcacccctc cgggaggagt tgtcccgctg
     9781 gggtttagcc aaggatgagt tcaccgataa cggcaactgg ccgtatcaca tctatgtccg
     9841 cgagggccgc cggttagccg gtgatttcgt gatcacgcag caggatgtca ccgaacgacg
     9901 tttttttgaa gacggcattg ctttgggcaa tttctatttg gacgtgcatc ccgtggatat
     9961 gttggtgaca cacgacatgg tggttgccga gggagggctg ggcaacgtgc gagtgaagcc
    10021 atacgaaatc ccctataggt cactgatccc aaagcgttcc gaggcggtga acctcctggt
    10081 gccggtttgc ctttccgcct cgcacatcgc gttctcgaca atccgcatgg agccggtatg
    10141 gatgatgctg ggtcacgctt gcggcgtcgc ggcgtcgctt gcacttgagt ccggcgcctc
    10201 cgtccaggat gtcgacggcc gcctgctgcg caaaagactc gccggcgatg gacagattgt
    10261 cgacgcgcgc ccattcacaa gaaagtggcc ctttcgttga gtgggacgtc gcggcgggaa
    10321 tgtggtgagc tcgcgggctc gccgtggctc agcgccgttc gcgaagcagc cgttccgctt
    10381 tgagaatagc cacgcagctc gccccgtggg tgatgtcgcc tcgcaggacc atttcgaccg
    10441 cctgggagaa cggaacggca accgtgtgga tgacctctcc ttcatccggg tcgggttccc
    10501 cgaggacggc gccgagcgcg agaaacatgt ggttgggcga ccgcaccgtg ctggtgaaag
    10561 gatcgagcac gccgagcgga atccattcgc tggccgtcaa tcctgcttct tccttgatct
    10621 cccgttgcgc cgcttcgagt gcggtctcac cctcatcgat cgctccgctg atgagttcca
    10681 ggcactcgcg ccctaccgcg tacttgtatt cccgcgtaag atacgctgtg tggtcgtccg
    10741 acagggcaag aacggttgag ccctccacca tttcgaggat gtcgaaatcg cgctcgcttc
    10801 ccccgggttg aatcacgtgg tcgcaccgca ggctgattcg cgctccccgg tacacctcgc
    10861 ggctcgacac gacttcgtaa ggtccccgtt tccctaacgg catcagaagc tccgcacacc
    10921 catctggagg atgtcgtcca cgcgtatggt ttcctttacg acgaacggtt cgccgtactt
    10981 cacgccaatc aggttgccgt agaagcgtgc gacggcggcg agccgttccc gtatctccct
    11041 ttctttcggg aacagttccg agcccgcctc caccgtgcca tattgcgacc cgtaggctag
    11101 tagtgcgtcc attcgcctgt cgaattggcg tgtaatgtcg acgacgaagg aaggggtgac
    11161 gttcgcatac aacgacgagt agatcacctt ggagggccgg tgcggctcgc tgtactcgtc
    11221 gagcttacgg agacccgcga ggaaacaggc ctcgtaagcg atttcaccgg cgcggtagtg
    11281 atcggggtgc cgcgcctccc agtaaggaag aatcaccacg cgcgggcgca agcggcggat
    11341 gtccaccgcc agcgtcatcc gcacactgat cgtattctcc agccgcgcgt cggggaagcg
    11401 tcggttgccg cgccaggaga gcagcattcg ctcccccgcc tcgccggctt ccagcagccg
    11461 gagctcaggg ctgccgcgcg ttcccatgtc accggccgtc aggtccagca cacccgtgcg
    11521 gtagcccatt tcggccatgc gaatcagagt gccgccgcaa gtctgctcca cgtcgtccgg
    11581 atgcgcggcg atggcgagaa cgtctacgga cggaacttct gtgagatcgg aaacaatcat
    11641 taccagtgaa ggtcgcgccg ccgtctgtag cgttcgcgcg tacgcatgac gttgatcagg
    11701 atgacaccgg cgacgaatcc accgacgtgt gcgaaccagg ctacgccgcc ctgcgacaac
    11761 tgcgaatagc ccacgctcca cataccgctt gccagttgaa tgaggaacca gtaggccaga
    11821 atcagaactg ccggaatttc caccgtggtg ataaatacaa aaacgacaac cagcgtcaga
    11881 atccgggaat gcggaaactt gatcaggtat gcgcccatga ccccggcaat cgcgccgctc
    11941 gcacctaccg tggggatgcg cgaatccaga ttggtcaccg catgcgttcc cgccgccgcg
    12001 agtccgcata gcaggtagaa caacaggtac ttccagtggc ccagtgtgtc ctcgacgtta
    12061 tcgccataga tccagaggaa ccacatgttg ccgatcagat gcatccagcc tccgtggaga
    12121 aacatcgagg tcagcatgtc cgtgtattcg aagcgggccg gtatcatgcc ccaagtcgag
    12181 atgaaagcgt tacgggtata gtcgtcgagc gacacttcgt atagaaatac gaagacgttc
    12241 accacgatca acagaatggt gacgaagggc gtggagtaac tcggttgagt gtcccgcagt
    12301 ggaatcatct agcgtgtcga ggtcacggta cgaacttcaa attcggcgtg agaacggcgt
    12361 tccgcgcgcg acatcagggc tgtcgcgtcc tccagtctcc gtatgccgcc tcgcgcgcca
    12421 agtgcggtgc agttcagggc agccatggcg ttggaaaaat cgagaagttg cgccatatcc
    12481 atgcccttca gcacagcata gcagaaggcg ccgtgaaata cgtcgccggc gccggtggtg
    12541 tcgacgcagt tcacgacgaa tgcgggcgaa tacacaaacc ggccttccga tcgcgccagc
    12601 gcacccaacg aaccgagcgt catcgccgcc accttcatgc cgtattcgtt ctgaatcatt
    12661 tcgagggcac gaaagggatc cctctcgttc gtccattgtg ttgggaactc ggaactggtc
    12721 accaggtaat cgaccagcgg cagcacgcga tcgaaaccgt gatagatggt atccacgtcc
    12781 acagtcaccg ggatggaagc ggatcgtgcg atggctgcag ctcgctccac cgctggagta
    12841 tcgtgcccgt cgatgtggag caggcgcgcg caggtgatct gttccggcgt gatctcttcc
    12901 ggtttgagac gaagacactc ctcgcgccgc cagaggacgg ttcgctcgcc ggttgtccgg
    12961 tcgatcagaa tataggcggt ctgattcgga cagttctccc ggacctgtac atgatcgatg
    13021 ttgatgcccg ttccggcgag gctttccagt tggatgcgcc cgcgttcgtc gtcccccacg
    13081 gttccgatat atttcacacg caggccgagg ctggcgcaag ttaccatcgc gctggcaacc
    13141 tggccgccag ggctcaggat ctcgtcttcg aatgccacct tgccggcgta ggccgggaac
    13201 ttgggcaata gcagcagcgt atctgtggcg ttcaggccaa ctccaacgac atcgaactca
    13261 gccattcagc tctcccctga tgcgctccaa cgcctcccgg gaggagacac tgccttgcgc
    13321 gagttgcgca ttgcctccgc cgcgcccgcc ggggccggca agcagcgatt tcagcaggtt
    13381 accggcgtgc agcccggtgg cggaggaaac cgccacgagc agcgacggcg gttcttctac
    13441 aaaagccgca aaaacggctt tgttccggga cgtgaagcct tgcgccagcg cgcgcagatc
    13501 gtcatcgata ggaccccggg cgatggtccg gctcgcgcgg cggacgccat ccgggcccgg
    13561 ttcggtcgct tcattcaaag cagcaccttc gatgcgcgcc aactcaatcg ataacttgtt
    13621 ccgtgctttc tcggattcgt tcaaacggcg tagctgcact gctacgaggg acggggtttc
    13681 gtccagcgag gcggaaagca agcggctgat ttccaacaac gcttcatagt ccaaccgggc
    13741 gcgccggaca gcccgcattc cgcagagaaa ctcgatgcga aggttgccgc gcatcttctc
    13801 caggcggcga atcagcaccg ctccgatttc accggtagag cggacgtgtg taccgccgca
    13861 ggcgctgcgg tcgaggccct cgatggtcac aatgcgcaat ttcccctggc gttcggaggg
    13921 tttccgtagc tcgagatttt ccgatgcatc ccggaattct accgcgagcg gccggttctc
    13981 gaaaacgatc tggttgacgc gttcctcagc ttttcggagt tgttccggct cgagcgatgc
    14041 aacgctcaaa tcgatggtgg aagccgcatc gccaagatga aagctcaacg tctccacgga
    14101 aaacagctcc atcagaacgg cagagagcag gtgctggccg ctgtgctgct gcatgtgatc
    14161 gaaacggcgc gcccaatcga tacggcagtt tacatcggag tgttcaaccg gcgtggagag
    14221 gatatgtgca atacggccgt cttcctcttc gatgacgtcc gtcaccattg cgttatcgat
    14281 gcagcctgta tcgtgcggct ggccgccgga ggcagggtag aatgcggtgc ggtccagata
    14341 gaggcggcgt ccgtcctccg agctttccaa aacgtgcgcc cggaattcgg tttggtacga
    14401 atcgttgtaa tagatccgtt cagtcaccgt tcgagcagat tagacccact cgagagcttt
    14461 tttcttgtag gcccagatat agccgacgat caggataccg aggaataccg caacctcggc
    14521 cactccaaac catcccaact ggcggtaccg tacggcccac ggaaacagga acacggcttc
    14581 cacgtcgaag atgacgaata agatcgcaat gatatagaac cggaccgtgt agcggccacg
    14641 cgccccgctg gaagcctgga ttccgcactc gtacgcctcc agcttggtga cggatggcgc
    14701 agccggacgg accagttttg ccaaaccgag cgcaacaatg ggaaacgcca gggcaaccgc
    14761 cataaatata aagatcggaa tatacccttc cggcatggct tcaatatatc acacggattc
    14821 gtaccgtttt catagtccgg atgcgaaccg gtgatcgtag cgcccttcca gaattttgtc
    14881 gaagttcatc gtcagaaaca ggttttccgg ctttccgtag agttcgaaaa cctggcgcgc
    14941 gctttcaaac tctttgggaa tgcgcgcgta gaaattcaag tgacgaggcg cgatcatcgc
    15001 cgccgcctcc ggcaggtcga gatagcgaag cacattcagg aagattggtc cctggacgtg
    15061 tgtgacaggg ggatcgatca acgtcacctg gtggatctgc ggatcgagaa cggcggcata
    15121 aagacccagc gctcccgaga cgccctggcc aacaaccata atacgagcgg ggtccacgtc
    15181 atcctggagc cgcagcacct gcacggcgcg catcacgtcc cacagccgca tggaatccac
    15241 cgtgtggccc acgtgcatcg cattgcgcag agtatctttc cagaagttct tcggccatgg
    15301 gatctctccg acgcccctgg gatacacaat cattctggga gcaatcgagc ggaaaactgc
    15361 attgatggag cgagcgtctt ccccgtcgga ggcgacgtac agcagcgcag gatgctttcc
    15421 gggcttttca tgcctgcgat acacggcgcg cacagtgatg ccttcctcgg attcgaagtc
    15481 gagttccagt tcgcggggcg gtgactccga aagggggcgc cgcaccttca agtcgaccgc
    15541 ggtaggcgga aacgccgcga atactttcct gcgcagcgtc tcaacgaccc ctgcgcgatg
    15601 ggcttcccag gaggcccggt tcgcataccg tttcaaccgc ggtgttggca gaaacgtatc
    15661 ctggatgcga tagttttgag catcggccgg tgaaccctct gggaacaccg ccaggctttc
    15721 cggcgccgca ttgctgtagt ccatgtcgag tttgcgttcg gggatgcgca tcaggtagcg
    15781 gtcgaaccag cggatcactt cttcgcgcag aaaatccgaa tcctggtggc cggtttcgac
    15841 gacaatgttc cggaaaccgg tgcgttcgcc ataactggaa tagaggccgc caaccttcgc
    15901 ttcgaactct tcatagccgg ggacgggaaa gagactgtcg agtttgccat gcgccatcag
    15961 cagcgggcgc ggtgcgatca gagatccctg gtgcatcatg tcgtgcagta gagcattaat
    16021 cgtaaacata caatcgcagt gtcctcgctg cgtgttcagt tgaacattcg cggcataagt
    16081 gctgatgccc atcaccggcg cggcgacttt cacgcgcggg tcgacggcgg cggtgaacca
    16141 ggtcatggcg gcgcccccgg agcggccggt catcccgatg cgatccgcat cgatttccgg
    16201 ccgggtttcg agataatcga tggcacgcat ggcgttccaa acttccaccc ccgcgggtgt
    16261 gtagccgcgc gagtaccagt catacatctc ctgggaatgg atgccgtgat ggacgccaaa
    16321 cgcctcggcg atctggatcg ggtcgatgat catcgccgcg tatccgttct tcgcaagcga
    16381 gatgccgtgg cgctggtacg cggccttgga gccgtgcggt tccatggcat ggccgcaaac
    16441 gtagatcacc gccggcgcct tccccgggcg gttcttcggc agatatagat ttgcggttac
    16501 gtagaatttc ggaatgctct cgaacgcgat gttctcgatg gtgtaggaac ccttatccag
    16561 cgtgttcgtg atctttacgt tcagcggggt ccgttcgggc cagggcagaa ggccgagtat
    16621 gtcgcgcatt tcttccagac gccgggcgcg gacacgctcc cactcttcgc gagaacggat
    16681 ttccgatgcc gcgcggtccg tgatgcggcg ggcgcatcgc tccaggtaag ccgttacggc
    16741 tgtcttgctc tcttccatgg agcgaagggc ggtgttgtct gtttgggcga acgccagtgc
    16801 gccaagaaac aggaccaaaa tacggatcat gttggtacga taacagacga atggggttga
    16861 taaccgtctc cggcgaaccg gggtgcaggt ttgaagaggc gggacggctt acggctcagc
    16921 ggctccgttg cgagtttgta acagagtcct ctcttgccgg catgttgatc gaggaattcg
    16981 ggccgcagat cgccatgccg gacaaggcgt acccgcacgt ggtgtcttcc atcgtcgcgc
    17041 gcatcgccac ccagcatcac gttgtcgttt gcctgcttgg cgccgagacg ttgttcagac
    17101 attaccccgg cgttttgaga gttcacgtcg atgctcccga accgcgccgg accgggaatc
    17161 tgatgctcga tggccgtctc acacgttccg ctgcgagaaa gcagttacga gaacggcaag
    17221 tgaagcagcg tgcggaaaga aagcggaaat tcggcagggc caccaccccg gctcacctat
    17281 tcgatctggt tctaaatgcc gacgggctcg aagcggaaga gatggccgag atcatggaga
    17341 ctgccgcgcg cgtgaaaggt gttttcgatc acgggttgct gggcgcttcc gctgaagcac
    17401 agcttcagtt ccaggtgcgc ctcgagctcg ccaaacacgg gattactccc atcggacacg
    17461 ctgagctcaa gcggcgggcc tttggccacc cgagtgaaga ggtttttgcc aacgtcctcg
    17521 atttttatcg cgtagcatgg aaatacgagc ccaagagctt tccgat
//
LOCUS       DASTMK010000418        13976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_534076, whole genome shotgun sequence.
ACCESSION   DASTMK010000418 DASTMK010000000
VERSION     DASTMK010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534076"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFQ79_17980"
     CDS             <1..491
                     /locus_tag="VFQ79_17980"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEU0141618.1"
                     /translation="NGGSRQEMNTPLRGGKATMWEGGLRVPMIARWPGRIPRGLVSTE
                     FLSTAEFLPTFAAVGGAATPKGVKLDGFNMLPVLEGKAKSRRTEFFWQRRSEKAARAG
                     EWKWIESERGGGLFNLSTDIGEKNDLSREKPDVLARVKNRWAAWRKEMDESEPRGPFR
                     DY"
     gene            677..1054
                     /locus_tag="VFQ79_17985"
     CDS             677..1054
                     /locus_tag="VFQ79_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0141619.1"
                     /translation="MARPKSPMLTDAELRLMEVLWDRGPSTVGEVTEALPKGVELAYS
                     SVLTTLRILEQKGYARHEKRGRAFVYAPLIRRRDAQKSAVRHLLGRFFEDSPELLVLN
                     VLEDERLSAAELRKLRKMIEEAE"
     gene            1051..2640
                     /locus_tag="VFQ79_17990"
     CDS             1051..2640
                     /locus_tag="VFQ79_17990"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEU0141620.1"
                     /translation="MSLPYSNVLFEAVLNGACRSVLFAVMAWTLLKALPGLGAGVRYV
                     VWYTTLVAALLFPAARLVTHWPVAEPPAQAFVTGFTARDLESVPAPAFQALPVTIPRG
                     WSAWPFFGVWLVISGLLMARLLRGVRHLRRLKAVSEEPPETLRRWSEQWLQEDSGGRN
                     ARLRISPQIRLPVTAGMRNPAILFPSSLASKLAEEDAHSIWLHETAHVRRWDDWTKLG
                     QRFLEAVLFFHPVVLWVGRRLQFERELACDEWVIRQTGRPLRYAACLTRLAEFASPES
                     CAAPALPMAKDRQQIFRRVEMLLHRDRKIHSLRPGVAGAITLVLLVAGAVSLTLTSPV
                     LVMAQAPEAPAEMPVSPQAPAPSGIAPQPSPAAAPQPGPESVRDDERKASQKASREQR
                     EKIQQLSIEIRRQVQEQIRPQVEEIRKLSEQIREKVQQQIRPATEKTKELTKQLQQQM
                     LSDNPDEAVMQKLQENIRQIQEQAVKAAETDIRTLEEKIHRHELNIKVPEEQIRQLEE
                     QIHKLEELIRIEHKVRRRASV"
     gene            complement(2665..3912)
                     /locus_tag="VFQ79_17995"
     CDS             complement(2665..3912)
                     /locus_tag="VFQ79_17995"
                     /inference="COORDINATES: protein motif:HMM:NF027658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HEU0141621.1"
                     /translation="MEYFHRKLRPYWRRARGAGPAEMLKAAVVDFESLRERCSRFDQE
                     LIEDLVAAGGPEYAALAVLAYRQTLAAHKLTVDWDGTPLYFSKENFSNGCIGTVDVTY
                     PSSPFFLLLNPQLLKAQLRPVLDYAQSPRWRWPFAPHDLGQYPLANGQVYGGAEESEE
                     NQMPVEESGNMLIMMAALARAEGNANFSRRYWPLLTKWAEYLRAKGMDPENQLSTDDF
                     AGHLARNTNLSIKAIVALASYGELAGQLGYNELAASYRDLSRQMASRWVEMADDGDHF
                     RLAFDKPGTWSQKYNLVWDKLLNLNLFPADVVRKEIAWYKQKQNAFGLPLDNRSTYTK
                     LDWILWTATLAGNQEDFRTFSQPAYRFANETPDRVPLSDWYWTLDGKQRGFRARSVVG
                     GVYIKMLENPATWRKWAGRAALN"
     gene            complement(3840..4766)
                     /locus_tag="VFQ79_18000"
     CDS             complement(3840..4766)
                     /locus_tag="VFQ79_18000"
                     /inference="COORDINATES: protein motif:HMM:NF028477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5127 domain-containing protein"
                     /protein_id="HEU0141622.1"
                     /translation="MKRFGAVRILPCLLAAAGCVTAQQTAPLRPPAIPLVTHDPYFSV
                     WSNTNELAASPTRHWTGSEQPLTGLIRIDGAVYRFLGGERRLPDSVQPMQQTGFELTP
                     TRTSYRFETSGIRLDLTFLTPALPDDLDILSRPATYLIWDIHATDGKEHQVKLYLDAA
                     SHLAVDRPDQRTVWSRHKVAGMDVLRVGSADQKILNRSGDDLRIDWGYFYIAVPWQND
                     KVETIGTTLPERENYAGTLELPALDSFDTPEYTSRAAPVIGFALDLGTITSRPVRRYA
                     ILAYDDIFPSSIFTASSVPIGEEREGPGPRKC"
     gene            complement(4772..6040)
                     /locus_tag="VFQ79_18005"
     CDS             complement(4772..6040)
                     /locus_tag="VFQ79_18005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0141623.1"
                     /translation="MQTRVLFCGLMAIPVLLSAQSREERIAKAVASLQDSLVAQRRDF
                     HMHPELSNREERTSKVIQEKLKALDFDDIRTGVSRHGIVALLKGGKPGPVVAIRADMD
                     ALPIEETLDVPYKSQNKGVKHACGHDVHMTVALGTAEVLSKMRNEIAGSVKFIFQPAE
                     EGAPEGEDSGAVRMIREGALENPKPKAIFALHTNPLIEAGTIGFSPGAVLASSDSLTV
                     RIIGKKVHAAWPHQGIDPVVVAAETILALQTIRSRRIDPVEPLVLTFGSIHGGNRHNI
                     IADEVRLEGTLRTHSEQVRERAITLVREITNGVAASHGASAEVTWSPRSNPPTVNDTA
                     LVEATLPIIRKTLGPENVLHLPPVMGAEDFAYFQKQIPGFMYWLGVGNKAKGITAMLH
                     TPDYDADESSLQVGVKVMTNIVLDYLDRNR"
     gene            6168..6974
                     /gene="mazG"
                     /locus_tag="VFQ79_18010"
     CDS             6168..6974
                     /gene="mazG"
                     /locus_tag="VFQ79_18010"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU0141624.1"
                     /translation="MDNDPSSTPGAKFQRLVDIMARLRSADGCPWDREQNFDTIKPYL
                     LEETYEVLDAIDARDWHNLADELGDLMLQAVFFAQMAAEQGSFRIEDSLDAINEKLVR
                     RHPHVFGDGTAQTAGEVIQRWEEIKADEREKQGKRVEGLLTSVPRSLPALVEAQQIAK
                     KAASVGFDWSTPEQVIEKLHEELEELSQARQSESPEQLEDELGDILFVVVNIARFFHV
                     NAEQALRKTNAKFRRRFGYLEQKLSEAGTSAADASLDQMEELWQQAKRQE"
     gene            6947..7699
                     /locus_tag="VFQ79_18015"
     CDS             6947..7699
                     /locus_tag="VFQ79_18015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0141625.1"
                     /translation="MATSQAAGMTEIRELTSNEEFVDAVRLQKEIWGFEEVDLLPVRL
                     FVVASKIGGQVLGAFDNSRMIGFCLSIPGVKPDGRSYLHSHMLGVRPEYRNSGLGRTL
                     KLRQRQDALARGIGLIEWTFDPLELKNAHFNLERLGAIVRRYVLNQYGSTSSPLHGGL
                     PTDRCVAEWWLAHPRVQAIVDGRTPDPAPVEATVSVPCDIARLKTENPKQARAIQEAM
                     SNQFLDYFSKGLAVIGFGRSEDAGTYLLGPWA"
     gene            7690..8805
                     /gene="menC"
                     /locus_tag="VFQ79_18020"
     CDS             7690..8805
                     /gene="menC"
                     /locus_tag="VFQ79_18020"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEU0141626.1"
                     /translation="MGLKIEKIILRQIKMPLVHFFETSFGRTTERHIILVEAVADGIS
                     GWGEVTAGENPFYNEEWTEAAWIILRDYVAPRVAGRELAGPEEVAPLSEHIRGHRMAR
                     GGLETAVWDLAARRNDVPLWSEIGGGDHKEIACGVSIGIQDSVAQLLEKIETELAAGY
                     QRIKIKIKPGWDVDVIREVRRRFPSIRLMADANSAYTLADSDRLRQLDEFYLMMIEQP
                     LSDDDIIDHAVLQSRLETPICLDECIRSARHAEQAIRLKACRIINIKLGRVGGFLEAR
                     RVHDLAARAGIPVWCGGMLESGIGRAHNIALSTLPNFTLPGDVSASKRYWAQDIIEPP
                     VEVTPRGTIVVSDEPGLGYSIDRDFIGRLLVREETVS"
     gene            8814..9746
                     /locus_tag="VFQ79_18025"
     CDS             8814..9746
                     /locus_tag="VFQ79_18025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0141627.1"
                     /translation="MKRRRPSSFRLYALISLMILLWSLNFTIGKIALREIEPLLLSAL
                     RTALAALFILPVYLWKVQTEKRRVEAEGPECLPRARQPWDRSELRMLILLGVFGVALN
                     QVFFVLGLGNTSVAHASVLMGMTPVQVLLLAAARKQERITLRKLLGMIIAVGGVAWLN
                     TSPGRGANASLVGDGFILLASFAFAVFTVAGKSVTKRHDSITVNTFAYVCGAILLLPL
                     IIWRGAGFDFSAVSGSAWVSLFYMAAFPSVLCYLIFYYALTFIAASRLSAFSYVQPFL
                     AMLLAFPLLGEPITSSAISGGALVLAGVYLTERG"
     gene            9746..11047
                     /locus_tag="VFQ79_18030"
     CDS             9746..11047
                     /locus_tag="VFQ79_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0141628.1"
                     /translation="MLSDFSRLLRGNRNYRYMWMGQVVTEIGDHFNNIAVFSLAIEST
                     GSGLVVTAVMLARAAAVILAGPLAGVALDRLDRRKIMIASDLMRGVVALGFILTVDRG
                     HTSMLYIFSALLMFASPFFTSGRASILPTIATKRELHTANSLTQTTQWMTLTIGTMLG
                     GTTVMQFGYSWAFVFNAASFLFSAWAISRIGGGAHFQAKRRALTESEVVRPWHEYTEG
                     LRYMRGFPLVLGIALIGVGWATGGGAAQILFTLFGEVVFNRGAAGIGIIWGCAGVGLL
                     IGGTLGHWLGKRIGFSAYKITISICYVVHGGAYVVFSQMQEFWLALVFIGLSRAAVAV
                     SSVLNYTQLLRHVSDEYRGRVFSTMESMIWATMMLSMMGAGVASQEWGPRTIGAWSGV
                     LSSTTAIFWAWANWSGRLPQPREEGTAPEEVEVHGDPRWTS"
     gene            11035..11766
                     /locus_tag="VFQ79_18035"
     CDS             11035..11766
                     /locus_tag="VFQ79_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0141629.1"
                     /translation="MDILMNTLKDKPVLVTGAAKRIGRSIALRLAREGARVLIHYHRS
                     EAEARQTAEECGGAPLFRANLESVPEIVRLFEQVEASRGPLYGLVNNAARFTRFDPME
                     IAEADWDFIHSVNLKGAFFCCQQAARLMRKNGGGRIVNVSSLGGLRPWAEHAHYCASK
                     AGLIMLTKSLAKAFAPAITVNSVAPGVIPFNDLNSESEAMVRNTPAKRPGTPDEVADA
                     VVYFLRASNFITGQVLAVDGGLNLR"
     gene            complement(11777..11863)
                     /locus_tag="VFQ79_18040"
     tRNA            complement(11777..11863)
                     /locus_tag="VFQ79_18040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11827..11829),aa:Leu,seq:cag)
     gene            11946..12803
                     /locus_tag="VFQ79_18045"
     CDS             11946..12803
                     /locus_tag="VFQ79_18045"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0141630.1"
                     /translation="MTIAITGATGQLGRLVIDKLKTKVPAGNIIALVRTPAKAADLAV
                     TVREADYRKPDTLDRALTGVDTLLLISSNKVGERAAQHRNVIEAAKKAGVKRMIYTSL
                     LHADTSAITSLAVEHSATEAELKASGIPFTILRNGWYTENYTASIPAAVAGGAFLGSA
                     GNGRISSAARADYADAAVAVLTGDGHESKVYELAGDEAYTLSDLAAEISRQTGKNIPY
                     KNLPEAEYAAALAGFGLPQPVAQAYASFDTGASKDALFDDGCELSRLIARPTTPLSVS
                     VADALKQLK"
     gene            12808..>13976
                     /locus_tag="VFQ79_18050"
     CDS             12808..>13976
                     /locus_tag="VFQ79_18050"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0141631.1"
                     /translation="MQPALRTLPRGVIALGFVSLFMDISSEMIHSLLPVFLVSVLGLS
                     TVAVGLIEGIAESTAAITKIFAGVASDWTGKRKPLVLLGYGMAALTKPLFPLATGAAT
                     VFTARFLDRVGKGIRGAPRDALVADLTPERMRGAAYGVRQSMDTIGAFAGPALAMALM
                     ALSSNNFRFVFWIAVLPAVISVGFAVFGVQEPDTHAAGANRRFPLQREEVSRLGFMFF
                     LVVTFAAILTLARFSEAFLLLRAAGVGLPAAYVPAVLIVMNVVYSASAYPFGHLSDRV
                     DRLLLLAAGIGFLIAADLVLAVANAVWLVLAGSMLWGLHMGATQGLLTTIVAATAPAD
                     LRATAFGIFNLVTGGALLVASVFAGFLWEAYGPAVTFLAGAIFSTVALIGLVALRS"
BASE COUNT         2700 a         4085 c         4263 g         2928 t
ORIGIN      
        1 acaacggcgg ctccaggcag gagatgaaca cacctctgcg cggcggcaaa gccacgatgt
       61 gggaaggtgg gttgcgcgtg ccgatgatcg cacgctggcc gggacgcatc ccgcgaggcc
      121 ttgtgtccac cgagttcctg agcacggcgg aatttctgcc cacctttgcc gccgttggtg
      181 gcgccgccac tccaaaaggc gtaaaactgg acggcttcaa tatgctgccc gtcctcgaag
      241 gcaaggctaa atcacgccgc acggaattct tctggcaacg ccggagcgaa aaagccgcgc
      301 gcgccggaga atggaagtgg atcgagtcgg aacgaggcgg cggcctcttt aacctttcca
      361 ccgatatcgg cgagaaaaac gatctctcac gggagaagcc cgacgtgctg gcgcgcgtaa
      421 agaaccgctg ggccgcctgg cgcaaggaga tggacgagag cgaaccccgc ggcccgttcc
      481 gggattactg acctgcgccc gagctttgca ctttgcgtac gcgacgagga gatccccggc
      541 accatggtca cctgcgctgc aatcttgctc tcgcaaacct gccgcttcat cttttaattt
      601 tttaattgac agcgctatct tttgcgagta cgctgaaact gagctattaa ttctttaaca
      661 gaatggaggt acttctgtgg cccggccgaa atcgccgatg ttaaccgatg ctgaattgcg
      721 gctgatggag gtgttgtggg accggggtcc ttccactgtc ggtgaggtga cggaggcgct
      781 tccgaaaggc gtagagcttg catacagcag cgtgctgacg accttgcgga ttctcgagca
      841 gaaaggatat gcgcggcatg agaagcgtgg gcgtgcgttc gtgtatgctc ccctgatccg
      901 gcgccgtgat gcccagaaaa gcgctgtgcg ccacttgctg ggaagatttt tcgaagattc
      961 gcccgaattg ctggttttaa acgtattaga agatgagcgg ctgagcgcgg cggaattgcg
     1021 caagctgcgc aagatgattg aggaggcgga gtgagcctgc cgtattcgaa cgtcctattc
     1081 gaagccgttc tgaatggggc gtgccggagt gtattgtttg cggtgatggc ctggacgctg
     1141 ctgaaggctc tgcccgggct cggcgcaggt gtgcggtatg tggtctggta cacgacgctg
     1201 gtagcggcgc tgctctttcc cgccgcccgc cttgttacgc actggcctgt cgcggagcca
     1261 ccggcgcagg cgttcgtgac cggcttcaca gctagagatc tcgagagcgt accggctccc
     1321 gcttttcagg cgctgccggt gaccataccc aggggctggt cagcctggcc gttcttcggc
     1381 gtgtggctcg ttatatcggg cctgctgatg gcacggctcc ttcgtggagt gcgtcactta
     1441 cggaggttga aggcggtttc ggaagaacct ccggagacgc tccggcgttg gagtgaacag
     1501 tggctgcagg aggacagcgg aggacgcaac gcgcgtctgc gaatttctcc gcaaatccgg
     1561 cttcccgtca cggcaggaat gagaaacccg gcgattctct ttccctcatc gcttgcctcg
     1621 aaactcgcgg aagaagatgc acacagcatc tggttgcacg aaacggccca cgtgcgccgc
     1681 tgggacgact ggacgaaatt gggtcagcgg ttcctggaag cggtgctttt tttccatccg
     1741 gtggtgttgt gggtggggag aagactccag tttgagagag agcttgcgtg tgacgagtgg
     1801 gtaatccgcc agacgggccg gccgcttcgt tacgcagcgt gcctgaccag gttggcggag
     1861 ttcgcttcgc cggaatcttg cgcggccccc gcgctgccga tggccaagga cagacaacag
     1921 attttcagga gagtagaaat gctgctacat cgtgatcgaa agatacattc actccggccg
     1981 ggagttgccg gcgccattac tctggtgctg ttggtggcgg gtgcggtttc actgaccttg
     2041 acgtctccgg tgctcgtcat ggcccaggcg ccggaggcac ccgccgaaat gcctgtttcg
     2101 cctcaggcac cggctccatc cgggattgca ccccagccaa gtccggcggc ggctccacag
     2161 cccgggcccg aatccgtccg tgacgatgaa cgcaaggcct cacaaaaggc ttcccgtgag
     2221 cagcgggaaa agatccaaca gctctctata gaaatccgcc ggcaggtgca agagcaaatc
     2281 cgcccgcagg tggaagagat ccgcaagttg agtgagcaaa tccgcgagaa ggtccaacaa
     2341 cagatcagac ccgcaacaga aaaaacgaaa gagctgacga aacagcttca acagcaaatg
     2401 ctttcagaca atccggatga agcagtgatg cagaagttgc aggagaacat caggcagatt
     2461 caggagcagg ccgtgaaagc ggccgaaacc gacatccgga ctctggaaga aaaaattcac
     2521 aggcacgagc tgaatatcaa ggtcccggaa gaacagatcc gccaactgga agaacagatt
     2581 cacaagctgg aggaactgat ccggatagag cacaaggtcc ggcggcgagc gagtgtctga
     2641 ggaatccggc ggtacgggcc gcaatcagtt gagcgcggcc cggcccgccc atttacgcca
     2701 cgtggccgga ttctctagca ttttgatata cacccctccg acaacagatc gggcccgaaa
     2761 gccgcgctgc ttgccatcga gcgtccaata ccaatcggac agcggcacgc gatccggcgt
     2821 ctcgttggca aacctgtacg ccggttgcga aaacgttctg aaatcctcct ggttcccggc
     2881 cagtgttgcc gtccatagaa tccagtcgag ctttgtataa gtggatcggt tatccagcgg
     2941 caggccgaac gcgttctgct tctgcttgta ccaggcgatc tccttcctga cgacatccgc
     3001 cggaaacaga ttcaggttta gcagtttgtc ccacaccagg ttgtacttct ggctccacgt
     3061 cccgggcttg tcgaacgcga ggcggaagtg gtcgccgtcg tccgccattt cgacccatcg
     3121 ggacgccatc tgcctggaaa gatcacgata gctcgccgcc agttcgttat agcccaactg
     3181 tccggccaac tcgccataac tcgccagagc cacaattgcc ttgatcgaca gattcgtgtt
     3241 gcgtgccaag tgcccggcaa aatcgtctgt gctgagctga ttctccggat ccattccctt
     3301 tgcccgcagg tattccgccc atttcgtgag cagcggccaa taccggcggc tgaaattggc
     3361 gttcccctcc gcccgtgcca gagcggccat catgatcagc atatttccgc tctcttccac
     3421 cggcatctgg ttctcctcgc tttcctcggc tcctccgtac acctgtccat tcgccagcgg
     3481 atactggccg aggtcatgcg gcgcgaacgg ccagcgccag cgcggggatt gcgcataatc
     3541 gagcaccggc cgcagttgcg ccttcaacag ttgtggattc agcagcagaa aaaacggtga
     3601 actcggatac gtcacgtcca cggtgccgat acacccgttg ctgaagttct ctttcgagaa
     3661 atacaggggc gtgccgtccc agtctacggt gagcttgtgc gcggccagtg tctgccggta
     3721 agccagtacc gccagcgcgg cgtactcggg accgcccgcc gccaccaggt cttcgatgag
     3781 ttcctggtcg aaacgggaac agcgctcccg cagcgattca aaatcgacga cagccgcttt
     3841 caacatttcc gcgggcccgg cccctctcgc tcttcgccaa tagggacgga gcttgcggtg
     3901 aaaatactcg atggaaaaat gtcgtcgtag gcgagaatgg catagcggcg gacgggccgc
     3961 gaagtgatgg tcccgagatc gagcgcaaag ccaatgaccg gcgccgcgcg gcttgtgtat
     4021 tcgggagtgt caaaactgtc gagggcgggc agctcgagcg tgcccgcata gttttcccgc
     4081 tccggcagcg tggtcccgat ggtctctacc ttgtcgttct gccatggcac cgcgatgtag
     4141 aaataacccc aatcgattcg caggtcgtct cccgaccggt tcaaaatctt ttgatcggcg
     4201 ctgcctacgc gcagcacatc cattccggct actttatgcc gcgaccacac cgtgcgctgg
     4261 tccgggcggt ccaccgccag gtggcttgcc gcgtcgagat acagcttcac ctggtgttct
     4321 ttcccatccg tcgcgtggat atcccagata aggtaggtcg caggccgaga caggatgtcg
     4381 agatcgtcgg gcagcgccgg agtcaggaac gtcaagtcga gccttatgcc ggacgtctcg
     4441 aaacgatagc tggtccgtgt aggcgtcagc tcgaagccgg tctgttgcat gggttgaacc
     4501 gaatccggca ggcgccgctc tccgcccaga aagcggtaga cggcgccgtc gattcgaatc
     4561 agtccggtca gcggctgctc ggaccccgtc cagtgacggg tcggactcgc cgccagctcg
     4621 ttcgtgtttg accacacact gaaatacgga tcgtgcgtca ccagcgggat ggccggcggg
     4681 cggagcggtg ccgtttgttg cgccgtgaca caaccggctg cggcgagcag gcaaggcagg
     4741 attcggacag ccccaaaacg cttcatcggg ctcagcgatt gcggtccaga taatcgagca
     4801 cgatgttggt catcactttc acaccaacct gtaagctcga ttcatcggca tcgtagtcgg
     4861 gcgtgtgcag catcgccgta attcctttgg ctttgttgcc aacccccagc cagtacatga
     4921 atccaggaat ctgtttttga aaatacgcga agtcttcggc gcccatcacc ggaggaaggt
     4981 gcaaaacatt ttccggcccc agagttttgc ggataatcgg cagagtcgct tccaccagcg
     5041 cggtatcgtt cactgtaggc ggattcgaac gcggtgacca ggtcacttcg gcggaagctc
     5101 catgcgatgc ggcaacgccg tttgtgattt cgcgcaccag cgttatggcg cgctcgcgaa
     5161 cctgctcgct atgagtccgg agagtacctt ccaacctcac ttcgtccgcg atgatgttgt
     5221 ggcggttgcc tccgtggatg gacccgaacg tcagcaccag gggctcgacg ggatcgatgc
     5281 ggcggctgcg gatggtctgg agcgccagga tggtctcggc ggcgacgaca accggatcga
     5341 tgccctggtg cggccacgcg gcatgtacct ttttgccgat gatgcgaacg gtaagcgagt
     5401 ccgaagaagc cagcacagcg ccgggcgaga aaccgatcgt gccggcctcg atcagcgggt
     5461 tcgtgtgcaa ggcgaaaatg gccttcggct tcggattttc gagcgctcct tcccggatca
     5521 tccggacagc acccgaatcc tcgccttccg gcgcaccctc ttcggcgggc tgaaagatga
     5581 acttgacgga tccggcgatc tcgttccgca ttttgctcaa cacctccgcg gtgccgagcg
     5641 ccacagtcat atgtacgtca tgcccgcagg catgcttcac acccttgttc tgtgatttgt
     5701 aggggacgtc cagcgtctcc tcgatgggca gcgcatccat gtcggcgcga atcgccacca
     5761 ccggtccggg tttgcctcct ttcaatagag cgacaattcc gtggcggctt accccggtcc
     5821 ggatatcgtc gaagtccaac gccttcagct tttcctgaat caccttggac gtccgctcct
     5881 cccggttcga cagttcggga tgcatgtgaa aatccctgcg ctgcgcgacg agcgaatcct
     5941 gtagcgaggc taccgctttg gcaattcgct cctctcgcga ctgggcgctc aataaaaccg
     6001 ggatggccat aagcccgcaa aacaaaactc gagtttgcat ggttttcgca ttaggttatc
     6061 acggcggaat tacagcctgg cgtgccggcg gaatcggaac gcggaaggta agcccggcct
     6121 ccggcatgtt aaatttaaga attcggctga ttctcccgca tttctaaatg gataatgatc
     6181 cgagttcgac tccaggcgca aaatttcagc gcctggtgga catcatggcg cggctccgtt
     6241 ccgctgacgg ctgtccctgg gatcgcgagc agaatttcga caccatcaaa ccttatctgt
     6301 tggaagagac ctatgaggtc ctggatgcca tcgatgcccg cgactggcat aacctcgcgg
     6361 acgagttggg cgacctgatg ctgcaagccg tcttttttgc gcagatggca gccgagcagg
     6421 gctccttccg catcgaagac tcgctggatg ccatcaacga aaagcttgtt cgccgccatc
     6481 cgcacgtttt cggtgacggg acggcgcaaa ccgccggtga ggtgatacag cgctgggaag
     6541 aaatcaaggc ggatgaacgc gaaaagcagg ggaagcgtgt cgaaggcttg ctgacctcgg
     6601 ttccccgttc gctgcccgcc ctggtggaag cgcagcaaat cgcgaagaag gcggccagtg
     6661 tcgggttcga ctggtccacg ccggagcagg tgatcgagaa gctccacgaa gaactggagg
     6721 agctcagcca ggccaggcaa agcgaatcgc ccgagcaatt ggaggatgag cttggagaca
     6781 tcctgtttgt cgtggtgaac atcgcccggt ttttccacgt gaatgccgaa caggcgctgc
     6841 ggaaaacgaa tgcgaaattc cgccgccgct tcggttacct ggagcagaag ctgtccgaag
     6901 ccggcacttc cgcggcggac gcttcactgg atcaaatgga ggaactttgg caacaagcca
     6961 agcggcagga atgacggaaa tacgcgaact tacctcgaac gaggaatttg ttgatgcggt
     7021 gcggcttcag aaggagatct ggggatttga ggaagtcgat cttctacccg tccgtttgtt
     7081 cgtagtggca agcaaaatcg gtgggcaggt attgggtgca ttcgacaact cgcggatgat
     7141 cgggttttgt ttgtcgatcc cgggagtgaa accggatggc aggagttatc tgcatagcca
     7201 tatgctcggc gtccggcccg agtaccgcaa cagcggcctt gggcgcacac tcaagctgcg
     7261 gcagcggcag gacgcgctcg cgcgtggcat cggcctgatc gaatggacct tcgatccgct
     7321 ggaactcaag aacgctcact tcaacctgga acggcttggc gccatcgtgc ggcgctatgt
     7381 gttgaaccaa tacggctcga cgtcgagccc gctgcacggc ggattgccca cagaccgttg
     7441 cgtggccgag tggtggcttg cacacccgcg cgttcaggcg atcgtcgatg ggcgcacacc
     7501 cgatccggcg ccggtggaag cgaccgtatc ggtgccgtgt gatattgctc gtttgaaaac
     7561 cgaaaacccg aaacaggccc gagccatcca ggaagcaatg agtaaccagt ttctcgatta
     7621 tttcagcaag ggcctggcgg tgatcgggtt tgggcgatcg gaggacgccg gtacgtatct
     7681 gttaggtcca tgggcttgaa aatagaaaaa atcatactgc ggcaaatcaa aatgccgctg
     7741 gttcacttct ttgaaaccag ttttggccgc acgaccgagc gccacatcat tctggtggaa
     7801 gctgtcgctg acggcatttc aggatggggc gaggtcacag cgggcgagaa ccctttctat
     7861 aacgaagagt ggacggaggc ggcctggatc attctgcggg attacgttgc gccgcgtgtt
     7921 gcaggccggg aattggccgg tccggaagag gttgcgcctc tctccgaaca catccgcggc
     7981 caccggatgg cacgaggcgg attggaaaca gccgtttggg atctggccgc gcggcgcaat
     8041 gatgttccgc tctggagtga gatcggcggc ggcgatcata aagagatcgc ctgtggtgtc
     8101 tccatcggta ttcaggattc cgtcgcgcaa ctgctcgaga aaatcgaaac ggagctggcg
     8161 gccggatacc agcgcatcaa aatcaaaatc aagcccggtt gggacgtcga tgtcatccgc
     8221 gaagtacggc gccggtttcc ttcgattcgt ttgatggcgg atgccaattc cgcttacacc
     8281 ctggcggatt ccgatcgtct ccggcagctt gatgaattct acctgatgat gatcgaacag
     8341 ccgttaagcg acgatgacat catcgatcac gcggtcctgc aatcgcgcct cgaaaccccg
     8401 atctgcctgg atgagtgtat ccgctccgcg cgtcatgccg aacaggccat ccgtctcaaa
     8461 gcctgccgga ttatcaacat caagctgggg cgcgtgggtg gtttcctcga agcccgccgg
     8521 gtgcatgatc tggccgcgcg cgccggtatt cccgtctggt gcggcggaat gctggagtcg
     8581 ggcatcgggc gtgcgcacaa cattgcgctc tccaccttgc ccaatttcac acttccaggc
     8641 gatgtatcgg cgagcaagcg ctattgggcg caggacatca tcgagccgcc tgtggaagtc
     8701 acgccgcgag gaacgattgt ggtgagcgac gagccggggc tgggatacag catcgatcgg
     8761 gattttatcg ggcgtcttct ggtgcgggag gaaaccgtaa gttgaggggc ggggtgaagc
     8821 gccgccgccc ttcctcgttt cgcctgtatg cactcatctc gctgatgatt ctgctgtggt
     8881 ccctgaactt caccatcggc aagatagcgc tgcgcgagat cgagccgcta ttgctgagcg
     8941 cattgcgaac cgcattggcc gccctgttta tcctgcccgt ttacttgtgg aaagtgcaga
     9001 cggagaagcg ccgcgtggaa gcggaaggac ccgagtgcct gccgcgcgcc cggcagccgt
     9061 gggaccggag cgagttgcgc atgctcatcc tgctgggtgt tttcggtgtg gccctgaacc
     9121 aggtgttctt tgtcctggga ttgggtaaca ccagtgtcgc gcacgcgtcc gtgctgatgg
     9181 gaatgacacc cgtgcaggtg ctgcttctgg ccgccgcgcg gaagcaggag cgtatcacgc
     9241 tgcggaaact tctgggcatg attatcgccg tcggcggcgt cgcctggctg aatacctctc
     9301 ccggaagggg agcgaatgcg agccttgttg gcgatgggtt cattcttctg gcatccttcg
     9361 ccttcgctgt cttcactgtg gccggcaaga gcgtcaccaa gcgtcacgac agcattacag
     9421 tgaatacgtt cgcctatgtc tgcggcgcta tcctgctgct gccgctgatt atctggcgcg
     9481 gcgccggctt cgatttcagc gccgtcagcg gcagcgcgtg ggtaagcctg ttctacatgg
     9541 cggcgtttcc gtccgtgctc tgctacctga tcttttatta cgcgctgacc ttcattgcgg
     9601 cctcgcgcct ctcagcgttc tcctatgttc agccattcct ggccatgctg ctggcgtttc
     9661 cgttgttagg ggagcccatc accagttcgg ccatttccgg tggcgccctg gtgctggcgg
     9721 gcgtttacct cacggagcgg ggctgatgct ttccgatttc tcgcgcctgt tgcggggcaa
     9781 ccggaactac cgttacatgt ggatgggcca ggtggtcacg gagatcggcg accatttcaa
     9841 taacatcgcc gtattcagcc tggccatcga aagcaccggc tccggacttg tggttaccgc
     9901 ggtgatgttg gcgcgcgccg cggccgtcat tctggcgggg ccgctcgccg gtgtcgcgct
     9961 ggaccggttg gaccgccgca agatcatgat cgccagcgac ctgatgcgcg gcgtcgtcgc
    10021 tctcggcttt attctgacgg tggaccgcgg gcacacttcg atgctgtaca ttttcagcgc
    10081 cctcctgatg tttgcctcgc cattcttcac cagcggccgt gcctccatcc ttcccaccat
    10141 cgccacaaaa cgggagttgc acaccgccaa ttcgctgaca cagaccaccc aatggatgac
    10201 gcttaccatc ggaaccatgt tgggaggcac caccgtaatg cagttcggct attcatgggc
    10261 cttcgtcttt aacgcggcgt ctttcctgtt ttccgcctgg gccatttcgc gaataggagg
    10321 cggagcccat tttcaagcga agcgtagggc actgacggaa agcgaagtcg tccggccctg
    10381 gcacgaatac acggaagggc ttcgctacat gcgcgggttt ccgctggtcc tcggcattgc
    10441 gctgatcggt gtggggtggg ctacgggggg tggcgcggcg caaatcctgt ttacgctgtt
    10501 cggggaagtg gttttcaacc gcggcgcggc cggcatcggc atcatctggg gctgtgcggg
    10561 cgtgggtttg ctgataggcg gaacgttggg ccattggctc ggaaaacgga tcgggttcag
    10621 cgcctataag atcacgatca gtatctgcta tgtcgtgcat ggcggagcgt atgtcgtctt
    10681 cagccagatg caggaattct ggctcgccct cgtattcatc ggcctttcgc gcgcggcggt
    10741 ggcggtcagt tccgtcctca attacactca attgctccgg cacgtttccg acgaataccg
    10801 cgggcgtgtt ttctctacga tggaatcgat gatatgggcc accatgatgc tttcgatgat
    10861 gggcgcgggc gttgcatcgc aggagtgggg accccgtacg attggagcat ggagtggtgt
    10921 gctgagttcc acgaccgcca tattctgggc ttgggcgaat tggagcggcc ggctgccgca
    10981 accccgggag gaaggcaccg cgcccgaaga agtggaagtt catggtgacc cgagatggac
    11041 atcctgatga acaccctgaa ggacaagccc gtactggtaa cgggggccgc caaacgtata
    11101 gggcggagca tcgctctgcg gctggctcgc gaaggggccc gggtgctcat tcattatcac
    11161 cgttcggaag cggaagcacg ccagacagcc gaagaatgcg gcggagcgcc gctgttccgg
    11221 gcaaacctcg agagcgtacc cgagatcgtc cgcctgttcg agcaagttga agcttcccgc
    11281 ggcccgcttt atggattggt aaacaacgcg gcccggttca cgcgcttcga tccgatggag
    11341 atcgccgaag cggactggga tttcatacac tccgtgaatt tgaaaggcgc gtttttctgc
    11401 tgccagcagg ccgcgcgcct gatgcggaag aacggcggcg gacgcatcgt gaatgttagt
    11461 tcgcttggag ggctgcgccc ttgggccgaa cacgcccact actgcgcgtc gaaggcaggt
    11521 ttaattatgc tgaccaagtc cctggccaaa gccttcgctc ccgccatcac cgtgaactcc
    11581 gtcgctcccg gcgtcatccc gttcaacgat ctgaacagcg agtccgaagc aatggtgcga
    11641 aacactccgg cgaaacgtcc gggcacaccc gatgaagtcg ccgatgccgt tgtctatttt
    11701 ctacgcgcga gcaactttat taccggccag gtgctcgccg tggatggcgg attgaactta
    11761 cgataaaggg gatttttggt gcggatgaga ggacttgaac ctccactccc ttgcgagaac
    11821 atggacctga accatgcgcg tctgccagtt ccgccacatc cgcacgtgtg agcctgcaat
    11881 tcgattttcc tgtactttta cccaggtgtc aaacttcgat tctacgctag gaaagggagg
    11941 aaatcatgac tatcgctatc actggagcta ccggccagct cggccggctc gtcatcgaca
    12001 aactcaagac caaggtgcct gccggaaata taatcgcact ggtgcgcaca cctgcgaaag
    12061 cggcggatct cgccgttacc gtgcgcgagg ccgactaccg gaagcctgat acactcgacc
    12121 gggcactcac cggagtggac acgctgctcc tgatctcttc gaataaggtc ggcgagcgtg
    12181 ccgcccagca ccgcaacgtc atcgaagccg cgaaaaaagc gggcgtaaag cgcatgatct
    12241 acaccagcct gcttcatgcg gacacttcgg ccattacgag cctcgcggtt gagcattccg
    12301 ccaccgaagc ggagttgaag gcttccggta ttccgttcac cattctgcgc aacggctggt
    12361 acacggagaa ctacaccgct tccatccccg ctgcggtcgc cgggggcgcc ttcctcggca
    12421 gcgcaggcaa cggcagaatc tcatccgcgg cacgtgcgga ctacgccgat gcggcggtcg
    12481 cggtgctgac cggcgacggg cacgaaagca aggtctacga actcgccggc gacgaagctt
    12541 acacccttag cgatttggcc gccgagatct ctcgtcaaac gggcaagaac atcccctaca
    12601 aaaacctgcc ggaggctgag tacgcagccg ccctggccgg tttcggcctg cctcaacccg
    12661 ttgcccaggc ctatgcgagc ttcgataccg gcgcatcaaa agatgcgtta tttgacgatg
    12721 gatgcgagtt gtcgcgtctt atcgcgcggc ctacaacccc gttgtccgtt tccgtcgccg
    12781 atgctctgaa gcagttgaaa tagggaaatg cagccagccc ttcgaaccct gccccgggga
    12841 gtgattgcgc tggggttcgt cagcctgttc atggatattt cttcggagat gatccatagc
    12901 ttactgcccg tctttctggt gagtgtgctt ggcttgagca cggtggctgt ggggctcatc
    12961 gagggcattg cggagtctac ggcggcgatc acaaagatct tcgccggcgt agcgagcgat
    13021 tggacgggca agcgcaagcc cctggttctt ttgggctatg gcatggcggc gctcaccaag
    13081 cccctgtttc ccctcgccac cggcgcggct accgtcttca cggcccgctt tctggatcgc
    13141 gtcggcaaag ggattcgggg cgcgccgaga gacgccttag tcgcggattt gacacccgag
    13201 cggatgcgcg gcgcagccta cggcgtccgc caatccatgg acacgatcgg agcatttgcc
    13261 ggacctgctc tagcaatggc gttgatggca ctatcgagca ataacttccg gttcgtgttt
    13321 tggatcgccg tgttgccggc tgtgatctcc gtgggattcg ccgttttcgg cgttcaggaa
    13381 ccggacacgc acgcggccgg tgcgaacagg cgttttccat tgcagcgcga ggaagtgagc
    13441 cgcctcggtt tcatgttctt cctggttgtc acgttcgccg ccatactcac cctggcccgc
    13501 ttcagcgagg catttcttct tctgcgtgcc gccggcgtgg gccttccggc cgcctatgtt
    13561 cccgccgtcc tcattgtcat gaatgtggtc tattccgcga gcgcctaccc gttcggccac
    13621 ctttccgatc gggtcgaccg cctcctcctc cttgcagccg gcatcggttt tttgattgca
    13681 gcggacctgg ttctcgccgt ggcgaatgcg gtgtggctgg tactcgccgg ttccatgctt
    13741 tggggattgc acatgggagc gacccaaggt ctgttgacca cgatcgttgc cgcaacagca
    13801 ccggcagatc tacgtgccac agcttttggg attttcaatc tcgtcaccgg tggagcattg
    13861 ctggtggcga gcgttttcgc cggatttctt tgggaagctt atgggcccgc ggtgacgttt
    13921 ctcgcgggtg cgattttttc gacagtagcc ctgatcggtc tggtagcgct acgttc
//
LOCUS       DASTMK010000419         5897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_212457, whole genome shotgun sequence.
ACCESSION   DASTMK010000419 DASTMK010000000
VERSION     DASTMK010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212457"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFQ79_18055"
     CDS             complement(<1..207)
                     /locus_tag="VFQ79_18055"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141632.1"
                     /translation="MARKVTANAPLTPAVLHILLALSTQERHGYGIMKQVEADSQGKV
                     KMGPGTLYGSIGRMIDAGLIRESDR"
     gene            complement(271..3693)
                     /locus_tag="VFQ79_18060"
     CDS             complement(271..3693)
                     /locus_tag="VFQ79_18060"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0141633.1"
                     /translation="MAKLSRDWDSRKPSYDFIVVGSGYGGAISAARLSAAPLDQKPSV
                     CLLERGKEWEVGSFPDTLTKYLDEIRDNDNPLGLYDMVNFKDISVIKGNGLGGTSLVN
                     ANVAIIPEEHVFQQSVWPRSMSLDALRPFYQRALDVLKPSRHPRFEQLKKLDALRKRA
                     AELGGSVDPLDITVTFENREDNGFGMRQPACTDCGDCVTGCNVGSKNTLYMNYLPMAA
                     RAGAEIFTQTKVEWIQKLVTGGWRVHGRHYRTRFSSSPFTLDAANVILAAGAVNSTEI
                     LLRSEMRGLRVSPRLGSGFSGNGDFFAISYNSDRFLQVLGFGNHPDSPGSATPPGPTI
                     TGAIRYNGSRPVLNRFLVEDVAFPSPVVRAAQIAFAGLRGDDTDTGDEAEERRRVLQD
                     LSQTNPYSPDGALNHTLLYLVTAFDDAKGSMEFEAPFWEPDGRLKIAWDDVGRQRIFS
                     LVNEELRRHARSIGATFVENPIWTAFDLRRLITVHALGGCPIGEDYMHGAVDEFGRVF
                     SGDGAVHDGLFVADGALIPSAVGVNPFMTISAVAERIAARKIEELQGNPYPAPKMAVS
                     MTGVGTAGVVSARDAELDRLFQRCSSKSIDALVNSGEKRIDLAARLIHNDTKWKGFLP
                     KGLLLGELAKRLFTGYDKKFWKEGGRYRGVTLYADGRIALNHVLEEITITERTNDLDP
                     GQYILLRYTDPGFEDLFYDVMKVIHDDLVLYRGYTGKYPNGRRGWTAPLLRSYEFEQM
                     TAADHRDLYSGGAPPSPADLEGDWRIDVVANANHATGIGWLRFDSKPDGRVEARCQFM
                     GVMESLALPKSFEDHFRLGHYAGAESEIRKIDGALLVGKWIHDVPSELLQALPADSLG
                     IWQVERANAGNRLAVYYMLTRAEAGDRPVNTLLQPFLERTLPQGVGMTFEEEMVGWYF
                     PGAAATEPGRAGDLKIRERIPSDWSRPAGAVEVRFKVTMVVADVNEFVEGAAHEARLK
                     GTITFGEFGETRDATFALDERRSFFNYLIQNPETGEAEMRYHIEFGSSGGARYTFDGR
                     KYMQQDENSGVRALAEILDDYTTLYVHVCEGERELGSGSMKFRTFEDLAATGSLLAFM
                     RSFRITGTDDLLVQTQARLKFLAFTGQFITQEYDPAGILLRTDVAGAGTGGV"
     gene            complement(3704..5122)
                     /locus_tag="VFQ79_18065"
     CDS             complement(3704..5122)
                     /locus_tag="VFQ79_18065"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141634.1"
                     /translation="MRKSPRSTVFALVLLPASFLFVFDKNQPERHEDIVEHFKYGSIG
                     AEERAGVPYEIWRVLPALFPEHLPQRSGKGYEKFGLLFETPDKARPIGSSYRQRQVAL
                     VGLNCAVCHTGVLRDAPDAPRRIILGMPAHQFDLQSYEEFFFACARDPRFTEDYILSG
                     IRSANPKFSWFDALLYRWFVIPRTKSGILEQSRNFAWFEGRPRQGPGRVDTFNPYKVM
                     FGFDMTGDHTIGTADLPSLWNQRVREGLWLHWDGNNGAVTERNKSAAIGAGASEASLD
                     LESMKRVEDWILDLKPPEFPRDKIDMGRIAAGKKLFDAHCSECHELGRPLIGQTIEQV
                     QIGTDPERVNSFTRELCDKMNTLGTGRPWKFSHFRKTEGYAAMPLDGIWLRAPYLHNG
                     SVPTLRDLLKPPEERPAVFWRGYDVYDYVNVGFVSDGVEAQREGFRFDTVLKGNRREG
                     HTYGTNLQAGEKDDLIEFLKTL"
     gene            complement(5115..>5897)
                     /locus_tag="VFQ79_18070"
     CDS             complement(5115..>5897)
                     /locus_tag="VFQ79_18070"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141635.1"
                     /translation="DRKDEYGGTLRNRARFVLEIVEAIRREVGRDFHLQVKISATEYN
                     NALSPFDPPGNTVKDSVPVAQWLEQAGIDALHVSAGSAFPHPRNPPGDFPVDEALKTF
                     DSMISSGTQELRNYLALRSEPLGKLYRALWLNARGDKIEGINLADSRAIKSAVSIPVI
                     CTGGFQTASVIRNAISGAACDAVSIARPLIANNDLVHLFANGLDQAPKPCTYCNKCLV
                     NVLENPLGCYEQSRFSSREEMIAEIMSVFDPAPFSEKISAHA"
BASE COUNT         1138 a         1878 c         1657 g         1224 t
ORIGIN      
        1 cctgtcgctc tcgcgtatca atccggcgtc gatcatgcgg ccaatcgatc cgtagagcgt
       61 gcccggtccc atctttacct tcccctgcga atcagcttca acttgcttca tgattccgta
      121 accgtgccgc tcctgcgtgg aaagcgcaag gaggatatgc agcacggctg gagtgagggg
      181 cgcattggct gtgaccttcc tggccatagc tctatgatat atccgtcacg gatataagtc
      241 aagagagtcg accgggaacg atagcaggcc ctacaccccg ccggtccccg cgcctgccac
      301 atccgttctc agcaggatgc ccgccgggtc gtactcctgt gtgatgaact ggccggtgaa
      361 ggccaggaac ttcaaccttg cctgtgtttg aaccagcagg tcgtccgttc cggtgatgcg
      421 gaaggaccgc ataaaggcca gcaggctgcc cgtggccgcc aggtcttcga acgttctgaa
      481 cttcatcgag ccggatccga gttcgcgttc cccctcgcag acgtgcacgt aaagcgtcgt
      541 gtagtcgtcg agaatttcgg ccagcgcccg cacgccggag ttctcatcct gttgcatgta
      601 cttccggccg tcaaaggtat atcgcgcccc accggaggat ccgaattcga tgtggtaccg
      661 catctcggct tcgcctgttt cgggattctg aatcaggtaa ttgaagaagc tccgccgctc
      721 gtcgagggcg aacgtcgcat cgcgggtctc tccaaactcc ccgaacgtga tggtcccttt
      781 cagccgggct tcatgcgcgg ccccttccac aaattcgttc acgtcggcca ccaccatggt
      841 cactttgaag cggacctcaa cagcgcccgc cggcctggac cagtccgagg gaattcgctc
      901 gcggatcttc aggtcgcccg cacggcccgg ctccgtggcc gcagcgcccg gaaaatacca
      961 gccgaccatc tcttcttcga acgtcatgcc tactccctgg ggaagcgtgc gctccaggaa
     1021 cggttggaga agcgtgttca ccggacggtc gcccgcttcc gcccgcgtca acatgtagta
     1081 caccgccagc cggttcccgg cgttcgcccg ctccacttgc cagatgccaa gcgaatccgc
     1141 cggcagtgcc tgtaagagct cagacggaac gtcgtgaatc catttcccca ccagaagcgc
     1201 gccgtcgatc ttccggattt cgctttccgc gccggcgtaa tggcccagcc gaaagtgatc
     1261 ttcgaagctt ttcggcaagg cgagcgactc catcactccc atgaactggc agcgagcctc
     1321 caccctgccg tcgggtttcg agtcgaatcg cagccacccg atgcccgtgg catgattcgc
     1381 gttggcgacc acatcgatgc gccagtcccc ctccaggtcc gctggcgacg gaggtgcgcc
     1441 gccgctgtac aaatcgcggt gatcggccgc cgtcatctgt tcgaactcgt agctgcgcaa
     1501 caggggagca gtccatccgc gtctcccgtt cgggtatttc cccgtgtagc cgcgatagag
     1561 aaccaggtcg tcgtggatca ccttcatcac gtcatagaac agatcctcga accccgggtc
     1621 ggtatatcgc agcaggatat actggcccgg gtccagatcg ttggtccgct ccgtaatcgt
     1681 gatctcctcg agcacgtgat tcaacgcgat acgtccatcg gcatacaagg taacgccacg
     1741 ataccggccg ccctctttcc agaacttctt gtcgtatccc gtaaacaggc gcttggccag
     1801 ttctcccagc agcagaccct tgggcaggaa acctttccac tttgtgtcgt tgtgaatgag
     1861 gcgcgcagcg aggtcgatgc gtttctctcc gctgttcacc agcgcgtcga tactcttgct
     1921 ggaacacctt tggaataacc ggtcgagttc ggcatcgcgc gcgctcacta ccccggcagt
     1981 tcccacaccg gtcatgctca ccgccatttt gggagccgga taagggttgc cttgcagttc
     2041 ttcgatcttt ctcgccgcga tgcgctcggc cacggccgag atggtcatga aaggattcac
     2101 gcccaccgcg gaaggaatca gtgcgccatc ggcgacgaac aggccgtcgt gtacggctcc
     2161 gtctccagaa aacacacggc cgaattcatc cacggccccg tgcatgtagt cttctccgat
     2221 aggacagcca cccagagcgt gaaccgtgat caggcgcctt aagtcaaaag cggtccatat
     2281 ggggttttcg acgaacgttg cgccgataga acgcgcgtgc cgccgcagtt cttcattgac
     2341 cagcgaaaaa atcctctggc gtcctacatc gtcccacgcg atcttcaggc gtccatccgg
     2401 ctcccagaac ggcgcctcga actccatgct tccctttgcg tcgtcgaatg cggtcacaag
     2461 gtacaggaga gtatggttga gcgcgccgtc cggagaatac gggttcgttt gcgacaggtc
     2521 ctgtaaaaca cgccggcgct cctccgcctc gtcgcctgtg tccgtatcgt cgccccgcag
     2581 tcccgcgaaa gcgatttgcg cagccctcac cacgggagag ggaaacgcga catcctccac
     2641 cagaaagcgg ttcagcaccg gccggctgcc gttgtaccgg attgcgccgg taatcgtggg
     2701 tcccggcggt gttgcgctgc ccgggctgtc cggatgattt ccgaatccca acacttgcag
     2761 aaaccggtcc gagttatagg agatcgcgaa aaagtcgcca ttgccactga accccgaacc
     2821 caggcgcggg gaaacccgca gcccgcgcat ctccgatcgc aacaggatct cggtggagtt
     2881 gacggcgccc gccgccagaa tcacgttcgc cgcgtccagg gtaaacgggc tgctcgaaaa
     2941 ccgggtgcgg tagtgccgcc catgcacgcg ccagcctccc gtaaccagct tctgaatcca
     3001 ttccactttg gtttgcgtga agatttccgc gccggcccgc gccgccatgg gaaggtaatt
     3061 catgtacagc gtgttcttcg agcccacgtt gcagccggtg acgcagtctc cgcaatccgt
     3121 gcatgccggc tggcgcattc cgaaaccgtt gtcttcccgg ttttcgaagg tgacggtgat
     3181 gtcgagcggg tccacggagc cacccagttc cgctgcgcgt ttccgaagag cgtccagctt
     3241 cttcaattgc tcgaaccggg gatgccggct gggtttcagc acatcgagcg cgcgctggta
     3301 gaacgggcgt agcgcatcca ggctcatcga gcgcggccag accgattgtt gaaagacgtg
     3361 ttcttcggga atgatggcta cattcgcgtt caccagcgaa gtaccaccca ggccgttgcc
     3421 tttgattacg gagatatctt tgaaattcac catgtcgtac aacccgagcg ggttgtcgtt
     3481 gtcgcgaatc tcatccagat atttcgtcaa cgtgtccggg aaggagccga cttcccattc
     3541 ttttcctcgt tccagaaggc agaccgaagg cttctggtcg agcggtgccg ccgacagccg
     3601 tgcagccgaa atcgccccgc cataacccga tccgaccacg ataaagtcat agctcggttt
     3661 tctgctatcc caatctcgcg aaagttttgc cataacacca tccctatagc gtctttaaaa
     3721 attcgatcag gtcgtctttc tcgccagcct gcaggtttgt gccgtacgtg tgcccttcac
     3781 gcctgttccc cttcagcacg gtatcgaatc gaaagccctc ccgttgggct tccactccgt
     3841 ctgaaacgaa tccaacgttc acgtaatcat acacgtcgta gccacgccag aatacggcgg
     3901 gccgctcttc cggcggcttc agcagatcgc ggagagtcgg tacggagccg ttatgcagat
     3961 acggcgctcg cagccatatc ccatccagcg gcatcgccgc ataaccctcg gtcttgcgga
     4021 agtgcgaaaa cttccacggg cggccggtgc cgagcgtatt catcttgtcg cacagctctc
     4081 gcgtaaagga attcacccgc tcggggtccg taccgatctg gacctgttcg atggtctgcc
     4141 ctatcaacgg ccgccccagc tcatgacatt ccgagcaatg tgcatcgaac agcttttttc
     4201 ctgctgcgat gcggcccata tcgattttgt cgcgtggaaa ctccggtggc ttcaggtcca
     4261 gaatccagtc ctccacgcgc ttcatactct cgagatcgag ggaagcctcc gacgcgccgg
     4321 cgccgattgc agcgctcttg ttgcgctcgg tgacggcacc gttgttgccg tcccaatgca
     4381 gccatagccc ttcccgaaca cgctggttcc acagagaagg caggtccgcg gtaccaatcg
     4441 tgtgatcgcc ggtcatgtcg aagccgaaca tgaccttgta tggattgaag gtgtccacgc
     4501 gtcccgggcc ttgccgcggg cgaccctcga accaggcgaa gttccggctc tgttcgagga
     4561 tgccgctttt ggtccgcggt atcacgaacc agcgatagag aagagcgtcg aaccatgaga
     4621 acttcggatt cgcggaccgg ataccggaca gaatgtaatc ttccgtgaac cgcgggtccc
     4681 tggcgcaggc gaagaaaaac tcctcgtagc tttgcaggtc gaactggtgt gccggcattc
     4741 ccaaaataat gcgccgcggc gcgtccggcg catcccgcaa cacgccggta tggcacaccg
     4801 cgcagttgag gcccaccaat gccacctgcc gctgccggta actggagccg atgggacgcg
     4861 ccttatccgg ggtttcaaac aggagcccaa atttttcgta tcccttcccg ctgcgctgtg
     4921 gcaggtgttc ggggaacaac gccggaagga cccgccagat ctcgtaagga acgcccgccc
     4981 gttcttcggc tccgatggag ccgtacttga aatgctcgac gatgtcctcg tgccgctccg
     5041 gctgattctt atcgaacacg aataaaaagc tggcgggtag cagcaccagt gcgaaaactg
     5101 tgctccgtgg gctcttacgc atgtgcggat atcttttcgc tgaacggagc gggatcgaag
     5161 acggacatga tttcggcaat catctcttcc cgcgacgaga accggctctg ctcatagcag
     5221 cccaatggat tctcgagaac atttacaagg cacttgttgc agtaggtaca cggtttcgga
     5281 gcctggtcca gaccgttcgc gaacaggtgt accagatcgt tattggcgat gagaggccgc
     5341 gcgatcgaca ccgcgtcgca agccgcgccg gagatggcat tgcgaatcac cgatgccgtc
     5401 tggaaccctc ccgtacaaat cacgggaatg gagaccgcgg acttgatcgc ccggctgtcc
     5461 gccaggttga tgccctcaat cttgtcgccg cgcgcgttca gccacagcgc gcgatacagc
     5521 tttccgagcg gttcgctgcg cagtgccagg tagttgcgca actcctgcgt gccgcttgaa
     5581 atcatggagt cgaacgtctt cagtgcttca tcgacgggaa aatcgcccgg agggttcctg
     5641 gggtggggaa aggcgctgcc tgcggagaca tgcaacgcgt cgatgcctgc ctgctccagc
     5701 cactgtgcca cgggaacgga atctttcact gtgttgcccg gcggatcgaa cggcgacaag
     5761 gcattgttat actccgtggc gctgatcttc acctggagat ggaaatcgcg gcccacttcg
     5821 cggcggatgg cttccacgat ttccagcacg aagcgcgcgc ggttgcgcag cgttccaccg
     5881 tattcgtctt tgcggtc
//
LOCUS       DASTMK010000420         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_502058, whole genome shotgun sequence.
ACCESSION   DASTMK010000420 DASTMK010000000
VERSION     DASTMK010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502058"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2409)
                     /locus_tag="VFQ79_18075"
     CDS             complement(<1..2409)
                     /locus_tag="VFQ79_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141636.1"
                     /translation="MPTSLERSGDFSRSFDTNGQPFVIYDPRTTRLDPNRPEGTTRYI
                     RDVFPGNRIPQGLINPIATAALGYYPAPNQAGLGQSDINNYFVAAADSLDGDRIDARV
                     DHQLTSTHTLFTRYNWFRNINAQPLVFEHFASPVETPNRIPGINWTLNHTWTIGPGTI
                     FEHHFSLAQSETNRIPLSMDFDQSTLNIPRNLIDGQRVKYFPRFTIGGLTQLGVTGTG
                     YNTVKSRTWQYAASMTMLRGSHTFKTGFDWRRFPVTIDQSSPLAFNSTGSFTAGPNPQ
                     AAAARTGRGLADLMLGISQVSYTLRPLEAHAHPYYAWYFQDEWKVKPKLTLTLGLRYS
                     LELPRTEENNVYVFLDMESPSPLSGQVPGFTDLRGGVGFVGVDGRGRRTQIADRTNWD
                     PRLGLAWEINEKTVLRSGFGVFTSSVVPNTDSSLGFSRTTSSLVAEPDGVTPLYNLSN
                     PLPGGFLPATGNSLGLLTNLGQGINGPVRQQRLPYQMQWSLDVQRQLPWQSMIEIGYA
                     GSSGVALPSGVQYNQLPVEYMDLGTALNQTVANPFYNIITDSTSSLSRPTVQRGQLLR
                     PFPQFTGTSANQAPVGHSSYHAMQMRVERRFSEGLAVLFAWTHSKLIDNVGDFGGFLG
                     AGGFTNNYCFPCDRSLSFYHIPDVVRLSFRYELPFGVGKPHLSRGVLARIVGGWSTAM
                     YFTWDNGTPVQVTGPNDSNSFGGGQRPDATGISAKIDDQKLEDGALWFNPAAFQRAPQ
                     FTFGTASRNLPDVRIPGFQNFDILIEKRISFTERYSLDFRTELFNAFNSVVFAGPQTN
                     VTSAD"
BASE COUNT          508 a          702 c          752 g          491 t
ORIGIN      
        1 atccgcggag gtcacgttcg tctgtggtcc cgcaaaaact acgctgttga acgcgttgaa
       61 aagctccgtc cggaagtcca aagaataccg ctccgtgaaa ctgatgcgct tctcgatcag
      121 aatgtcgaaa ttctggaaac cgggaatacg cacgtccgga agattgcggc tggctgtgcc
      181 gaacgtaaac tggggtgcac gctggaatgc tgccggattg aaccacaacg caccgtcttc
      241 gagtttttga tcgtcgatct tggcgcttat accggttgca tccgggcgct gcccgccccc
      301 gaacgaattg gaatcgttcg gaccggtcac ctgcaccgga gtgccattgt cccaggtgaa
      361 atacatcgcc gttgaccatc cgcccacgat gcgtgccagc acgccccggc tgagatgcgg
      421 cttgcctact ccgaacggca actcgtagcg gaagctcaag cgcacgacat ccggaatgtg
      481 gtagaaactc agcgaacggt cgcaagggaa gcagtaattg ttggtgaaac cgccggcgcc
      541 aaggaagcct ccgaaatcac ctacgttgtc gatgagcttc gaatgggtcc acgcgaacaa
      601 caccgccaga ccttcggaga atcgccgctc cacacgcatt tgcatggcgt gatagctgga
      661 gtgtcccacc ggcgcctggt tcgccgacgt gcccgtgaat tgtggaaacg gacgcagaag
      721 ctggccacgc tggacggtcg gccggctgag agacgaggtc gagtcggtga tgatgttata
      781 gaacggattc gccaccgttt gattcagcgc cgtaccaaga tccatgtact ccaccggcaa
      841 ctggttgtac tgtacgccgc tggggagagc aacacccgag gagcccgcgt atccgatctc
      901 gatcatcgac tgccagggaa gctgccgttg tacatccagg gaccactgca tctggtatgg
      961 cagccgctgt tggcgcacgg gtccgttgat accctgtccc aagttcgtca gaagccccag
     1021 cgagtttccg gtggctggaa ggaatccgcc aggcaatggg ttggagagat tgtagagcgg
     1081 cgttactccg tccggctccg ccaccagaga ggaagtggtc cgcgaaaaac cgagggagct
     1141 gtctgtgttc ggcaccaccg acgaggtgaa aacgccaaaa ccggaacgta ataccgtttt
     1201 ctcgttgatc tcccaggcta agccgagccg tgggtcccaa tttgtcctgt cggcgatttg
     1261 cgtccggcgg ccccggccat cgacgccgac gaaacctacc ccgccgcgca ggtccgtgaa
     1321 tcctggaacc tggccgctca gtggtgaagg cgactccatg tcgaggaaca cgtacacgtt
     1381 attctcttcc gttctcggaa gttccaggct atagcgcaag cccagggtga gggtaagctt
     1441 cggtttcacc ttccactcat cctggaaata ccaggcgtaa tagggatgag catgcgcttc
     1501 cagcggacgc agtgtgtagc tcacctggga gatgcccagc atcaggtcgg caagccccct
     1561 gcccgttcgc gccgcggcgg cctgcgggtt cgggccggcc gtaaaggagc cggtcgaatt
     1621 gaaagccaac ggagacgact ggtcgatggt cacggggaag cgccgccaat caaaccccgt
     1681 cttgaaggta tggctgccgc gcagcatcgt catcgacgcc gcatactgcc aggtgcggga
     1741 cttcaccgtg ttgtaaccgg ttcccgtcac cccgagctgc gtcagcccgc cgatggtgaa
     1801 ccgtggaaag tatttcaccc gctgcccgtc gatcaggttc cgtggaatgt tgagggtgga
     1861 ttgatcgaaa tccatgctga gcggaatgcg attggtctcg ctttgcgcca gtgaaaaatg
     1921 atgttcgaag atcgtgcccg gcccgatggt ccaggtgtga ttgagagtcc agttaatgcc
     1981 tggaatgcga ttcggtgtct cgaccggaga agcgaaatgc tcgaatacca gcggctgcgc
     2041 gttgatattg cgaaaccagt tataacgagt gaacagagtg tgcgtggacg tgagttgatg
     2101 atcgacgcgc gcgtcgatac gatcgccatc gagcgaatcg gcggccgcta cgaagtagtt
     2161 gttgatatcg ctttgtccca accccgcttg attcggtgcg gggtagtatc caagcgccgc
     2221 cgtggcgatg ggattgatca gtccctgcgg aatccggttg ccgggaaaca cgtcccggat
     2281 atagcgcgtg gtgccctcag gccggttggg atcgagcctc gtcgtgcgcg gatcgtagat
     2341 cacgaaaggt tgtccgttgg tatcgaacga ccgcgagaag tctccgctgc gttccagtga
     2401 cgtcggcaca gtgcctgtgt aggcgcgcag gctcctctgg cgcagccctt cgt
//
LOCUS       DASTMK010000421        24661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_502114, whole genome shotgun sequence.
ACCESSION   DASTMK010000421 DASTMK010000000
VERSION     DASTMK010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24661
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502114"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFQ79_18080"
     CDS             <1..1112
                     /locus_tag="VFQ79_18080"
                     /inference="COORDINATES: protein motif:HMM:NF021156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Cas7"
                     /protein_id="HEU0141637.1"
                     /translation="KLAIIAPTSLVFGVWDSRSTQVKQQRVFRANIRATNVREVSRSA
                     QYTPATAYVDVGAISEDIEKKSGDESNLSQEGMVHALSTQTVGGVMLTGESKLVRTVN
                     VNLEAIRALRGTVPSPTSVQTQPETGGEQRSAGTAESAQPIDGTEALQRYILGLALVA
                     ATHEPELNLREGCHLRFKDGATHSIKLIYRSKPGMEQELPEDVLSFAETAKSNFFKLA
                     GIDEETMHRRDAVLETNIAERFLSYPQGDRDKIRKLGPITQATLDRFKALGNDPFKTV
                     KDELKAMSKDLGKKPRKNAPPVKKIEAFRKVAELLDQLAENEALPEGVADLASELAKT
                     AREHDNSHDALKHIEEEIKNYKKAQKESTPTGSNT"
     gene            1121..2704
                     /gene="csb2"
                     /locus_tag="VFQ79_18085"
     CDS             1121..2704
                     /gene="csb2"
                     /locus_tag="VFQ79_18085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HEU0141638.1"
                     /translation="MTFLRVTVWFFDDRYHGLLDPTGPAEWPPSPFRLFCALVAGVAR
                     RGELERETGRVLAWLQNLEPPIIIAPRSKMGRAIRRYVPNNDGDNPRKLDRKDRLTEK
                     LTIPTLMSVNPDEMPKVHYLWTIKDKTAVPMDRIRHAAHSLTALGWGVDIAFADASKA
                     SEADIQALQGVRWHPKPGVRRDQGMLRVPTAHSNFQECTLCDLKHCHQTATERIEHGK
                     PLHTVDKPRVFERVFYASVEQPIGRPCRVFELRNVDGESERYPQRKLVHLAGMVRHLA
                     AQAMTKDPPHGVGEDWADTFVAGHWRRGEPASLKRNECVDDADRRQQGEELTTVDKHR
                     QLSYLPLPSIGRVHTDPGVRRIMITAPVGDDAWLDYVARHLAGQMLKPLRGDEFGGDG
                     PPLLVPVRHDDIARLYTKQANIWASVTPVILPGHDDHKPEKTHKLIERALAQSGIEQP
                     CEFEWSAFSRFPKSLSAHKYDKNKKPTGYLRPDYLNSQTAVHLTLRFSNGLSAPGPLV
                     IGAGRHIGLGLMAPRENWD"
     gene            complement(2743..4503)
                     /gene="cysN"
                     /locus_tag="VFQ79_18090"
     CDS             complement(2743..4503)
                     /gene="cysN"
                     /locus_tag="VFQ79_18090"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEU0141639.1"
                     /translation="MAVTAPMTAEEGIEAFLRQNETKELLRFTTAGSVDDGKSTLIGR
                     LLYDSKGVYEDQLASVQKASVNRNAGPIDFSLLTDGLRAEREQGITIDVAYRYFSTPR
                     RKFIIADTPGHEQYTRNMATGASTADLAVVLVDARKGVLPQSRRHAYIAALLGIPRIV
                     VAVNKMDLVDFDEQIFLTIRSDFQAFAAPLEIPHLDFIPISALDGDNVVGRSERTPWY
                     SGPALLEYLETVPATAARNLDQFRLPVQYVIRPNLDFRGYAGQVASGVLRPGDRLMVL
                     PSGRTSRVKSIVTYDGELREAFPPMSITVCLEDEIDISRGDMLVSPDSMPHASRRFNA
                     SVVWMNADPLEPGRPYVLKHTTQQTSGTVRTVLETVDVNTLEKSPASVLHLNEIGRVA
                     IETHKPLFFDSYRRNRSTGSFVLIDPVTNATVAAGMIEDTSRRTPADEAAKAAISALE
                     SGSSRVTPAERYARAGHRPATLWITARKEAAYHLERMLFDRDCLVKVITDDFESPLVS
                     ELARFSNHGGFIAICSVSSNAPEETARAREVVGADKFIEVDPTELSPRDEEAAAQLCR
                     LLETRGIIRRDDRFAEGSGI"
     gene            complement(4503..5420)
                     /gene="cysD"
                     /locus_tag="VFQ79_18095"
     CDS             complement(4503..5420)
                     /gene="cysD"
                     /locus_tag="VFQ79_18095"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEU0141640.1"
                     /translation="MIDFMQLPTLTHLRALEAESIHILREVAAEFQKPVMLYSIGKDS
                     SVMVRLAQKAFYPGPIPFPLLHVDTTYKFREMIAFRDSFTARIGARLIVHTNTAAIEE
                     GANPFRLGTQKCCGLLKTRALLDAIAQGGFDAAFGGARRDEEKSRAKERVYSFRDAYG
                     QWDPKNQRPELWNLFNSRIDKGESIRVFPLSNWTELDVWQYIHLEKIPIVPLYFAKER
                     PMVVRGDSLIPLEHDIPLLPGEKPQMVMCRMRSLGCTPCTGAIRSEADTVPKIIEELM
                     LFRRSERENRIIDHDQEGSMEIKKREGYF"
     gene            complement(5464..7455)
                     /locus_tag="VFQ79_18100"
     CDS             complement(5464..7455)
                     /locus_tag="VFQ79_18100"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0141641.1"
                     /translation="MPMLRGFLFLLFATAAWAAQPFYDRTHPSKVFGQDRHYRILLPP
                     NYQGSGKAYPVIYYFHGHSDRYTLEKYDNGKDTIPKMAGFVSKNGCIVVAVDGYVARD
                     YTGFYGGSPWDVREEGGDYDFGEYFKELVAHIDSTYRTLTDRRHRATSGLSMGGFMSL
                     WLSARYPDLIGSASAFNPGPEFYTGDKGRRILWRPKDHVSNHGHTMVRLIRASGDYIS
                     QYHEETHEAYARAREVDYEYRRDEYHRHWATSIGETFGFHMRAFGSSVLDNVPEVWSH
                     ANPYRRFQVWGYEFSSEGEQPGTVYLEAVYQGGMRITTRRWAPDGPPAERRIRVLTAP
                     LYRTGASYNLTDYNLTTGTAETRQIVPDDKGRLSFTVDGQGHQLSFAGPGTGSQPSVL
                     LPLTRKEVLRLPPGEEIALPIRIYNPRSVPMTAVKAALSSQYPTVQVLSGSGDIPKIE
                     PGQAADLSSHMKVRFVAGAGYFTHARLELRLTYDGWQSTQENIDVLIAPETIPNPDAV
                     EVLDGRTFTFNVFHQKGNQGGGASVQRTVTEGSGNGNGVLEPGETATIWVRMKQGMDP
                     FDKNNWYRTKIYTDSNYVDETGDIQEQKQLEWTGAKERTSLIRLAPATPAGTSIPLLL
                     DNESWSYHYTPDVRYGKEKLYQAFQLHTRHLHRFVLQVP"
     gene            complement(7455..8801)
                     /locus_tag="VFQ79_18105"
     CDS             complement(7455..8801)
                     /locus_tag="VFQ79_18105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU0141642.1"
                     /translation="MMNRREFLASSAAGLLGAETTASKPNIIFLLADDLGYGDLSCYG
                     QKRFSTPNLDRIAAEGTRFTQAYAGSTVCAPSRCCLMTGLHSGRATVRGNKKPEVGLR
                     PEETTVASLLQKAGYETALFGKWGLGGPATGSVPNTRGYDHFYGYLNQLHAHNYYPSH
                     LWDNQNEAFLTENWFNQRKQYSPDLIHRRTLDFLEQDRAKPFFLYLAYTIPHANNELG
                     RYQGNGMEIPDNAPFSGRDWPETEKNFAAMVTRLDREAGDILDALKRKGLDSNTLFIF
                     SSDNGPHREGGHDPEFFNSNGPLRGIKRDLYDGGIRVPFFARWPGRVPAGRTSDQVFA
                     FWDLLPTAAELAGASAPPGIDGASVLPALLGNRQTSHEYLYWEFHEGGFKQAVRMDEW
                     KGVRLSADGPVELYNVVEDVGEMKDLAAANPQVVKKIGQIMTHARTESTLFPVQKS"
     gene            complement(8862..9470)
                     /locus_tag="VFQ79_18110"
     CDS             complement(8862..9470)
                     /locus_tag="VFQ79_18110"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HEU0141643.1"
                     /translation="MAKVLVAALVFCAAIPMAASGGALALRAARNSQVIQNKRADRDD
                     LSRMKNVAMVKRFAKAGLLVRVPSSTRRYYLHRVPHNYQYLRPWSKLFLERLSKQYFR
                     KHNQRLRVTSMVRTVEYQNSLRLRNVNAAPAVGFFRSTHLTGATLDISKKFMKPEGVK
                     WMRRVLQSLVLKGFLSAVEEFRQPTFHIMVHRNYPKYVASLK"
     gene            complement(9862..10119)
                     /locus_tag="VFQ79_18115"
     CDS             complement(9862..10119)
                     /locus_tag="VFQ79_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141644.1"
                     /translation="MIRDPELETAVLHFVETFTLVFDHDWDMTKGIITDPWYISPSGT
                     FINPKVTDESNNWANRGALLNAYRNLLALLKERDLIPDFAA"
     gene            10245..10841
                     /locus_tag="VFQ79_18120"
     CDS             10245..10841
                     /locus_tag="VFQ79_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141645.1"
                     /translation="MKPLLPIVLIVLTALAGMAQQGRVGRGQPPPNQGSALLHITPTP
                     SQIFSSLKDLAAASLLIVEGTVKATLPSRETSPRALETDAVIAVRRVLKGPADVREVV
                     ISQRGGSRGGLVVMPAQYSLVQPGEHYVLFLTEDSRPDIPDVPGVRRYLVTGLWTGLF
                     RFQEGKMHTDARKVDPLRAKYEGVSAEEIIEEVKASLW"
     gene            complement(10854..12269)
                     /locus_tag="VFQ79_18125"
     CDS             complement(10854..12269)
                     /locus_tag="VFQ79_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0141646.1"
                     /translation="MPNDDAIIVGAGPNGLSAAIELVRAGLSVSVYEASDVVGGGVRS
                     AELTLPGYTHDVCSAVHPLAYASPFFRRLPLGQFGLEWINPDAALAHPLEADECVVLE
                     RSVSVTSASLGVDRQAYSEFFSSVLEHWSRIETAFLHPFHLLRYPGSLARLGMDAFRT
                     ARGLAHRQFQGTKARALFLGLSAHSMLPLDRIPSAAFGLVLGLYGHAYGWPVPRGGAQ
                     KIADALAAYLRSLGGRIVTGNRVDSLSELPSTRIVLCDVTPRQLLRIAGESLPSGYRR
                     SLERYRYGMGAFKVDWALREPIPWRSPRCAEAITVHLGALPQEIEASERAAWAGGLCE
                     RPFVLLAQPSLFDRDRAPAGRHTAWAYCHVPHASDADMLSRIENQVERFAPGFRDCIL
                     ARSVKSPRELERGNPNLVGGDINGGVADWRQLLARPTWRLYRTPVKGLYLCSASTPPG
                     GGVHGMCGYYAARAALHDLKK"
     gene            12393..12710
                     /locus_tag="VFQ79_18130"
     CDS             12393..12710
                     /locus_tag="VFQ79_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141647.1"
                     /translation="MSKYRVVFMERTDQFGENIESPAEYLMPQLSDGVVIDTEFVSRN
                     QPEAQHSQEVLDEDDSFLSIGTEIWMYEIADGREQEFEDAARNSRMVIELERMDDTEM
                     IGS"
     gene            complement(13089..15038)
                     /locus_tag="VFQ79_18135"
     CDS             complement(13089..15038)
                     /locus_tag="VFQ79_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141648.1"
                     /translation="MQHTLLAGPAIVSSFLLLSCPTLFAADSVTAMRLKVERPTLLCL
                     GFEWQITGDDNRNASVEVSYRRVGEQAWQKGMPLLRLGGEKVIRPDLGLDYTVPDMFA
                     GSILDLQPDTAYEARFELRDPDGVNGAAVQTAKVRTRAEPKAASGGRILHVYPPGWTG
                     AKEQPAFTGLKQAYFGSGNGDWAVVSERKVRPGDTILVHAGLYKGDRLQYSHPLGLDF
                     TGTYVLTAKGTPEQPIVIRGADDGEAIFDGDGAHELFNVMGADYHIFEGLTIRNADIA
                     FQAGLKDLIGAKGLTVRSCRIEDVGIGVTTQYSGSHDFHISDNVMLGRDDRYRLRGWY
                     NPGIYGRNLLNSYYAVKVYGAGHVVSHNYIAYFHDGISVCTHGSPDPEEDRKASSIDF
                     YNNDIHLMADDFIEADGGVHNIRIFRNRGVNAAQCGLSAQPVYGGPAYYIRNVVYHVP
                     TGCGLKFNVKPSGLVLYHNTIIAEGLPGDLFSNAHFRNNLFLGTDAPGRAVLRFSNAT
                     AYSTYDYNGYRLNPQAKDQFLWISPAADALRDYTLNRGSAKRFGSLAELRTVTGQEAH
                     GIEVDYNIFEDLRAPDAAKPQAVYHASELNFKLNPHGKAVDAGIHLPGVNDGFVGNAP
                     DLGAYEVGQPLPKYGPRSTAATAYE"
     gene            15144..16415
                     /locus_tag="VFQ79_18140"
     CDS             15144..16415
                     /locus_tag="VFQ79_18140"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141649.1"
                     /translation="MNSRRTFLIGTGAAASQVLVRKAGANDRVRVAVLGVNGRGRDHI
                     EGFQGVPGTEVALLCDPDSKLLGERAAQFQEKYGRKVETANDLRKVFERKDIDAVSVA
                     TPNHWHSLAGIWACQAGKDAYVEKPASHNVFEGRKLVEAAHKYKRIVQHGVQLRSSDA
                     IQEAVQLLRKGVIGKVYMARGLVYRWRPSIGHKPTEPVPSYLDYNLWTGPAQQRPFSR
                     RLVHYNWHWHWDYGNGDVGNQGIHETDMCMWGLDVGLPKRITAMGGKFLFDDDKETPE
                     ILTSVYHYPEEKKIIQFEVRHWCTNEEHGVGVGNIFYGSEGYMLIRGYNTYEVYLGQK
                     REKGPSGKSGGDHYANFIKAVRSRKTSDQNGPVETAHLSSALAHLGNISFRLERQLTF
                     DPATEKFIGDSEANSMLTRNYRAPFVVPEKV"
     gene            complement(16449..16760)
                     /locus_tag="VFQ79_18145"
     CDS             complement(16449..16760)
                     /locus_tag="VFQ79_18145"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0141650.1"
                     /translation="MSEIRRLSFLLICVLFALAWPTVASQKFEISDDAIYDQVRRRLA
                     NDPDVKGGTLGVEVKEGVVTLRGTVEKEKLKQKAEKLTKKVPGVKRVINELKIEPVRT
                     S"
     gene            complement(16753..17703)
                     /gene="era"
                     /locus_tag="VFQ79_18150"
     CDS             complement(16753..17703)
                     /gene="era"
                     /locus_tag="VFQ79_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU0141651.1"
                     /translation="MPKRRSPDGSPSKKKPHVSGFVSILGLPNAGKSTLLNALVGTKL
                     AIVADKPQTTRTSVQGVLTLPDAQIVFLDTPGIHKSDSLFNRKMMQTVRAAVEDRDLL
                     LFLVDVSRPHSIEEEKALDLVRKGQAPVFLLLNKIDKVKDKRKILPRIEQLRTLLDFA
                     EYIPISAATGDGLEDLVKAMIAGLPEGPPLFPGDYLTDQPERFLAAEIIREKVLELTR
                     QEVPHSVAVLVETWEETGNLLRIAATIYVEREGQKAIIIGSKGAMLKEVGTLARQELE
                     SFFGRKVFLELFVKVRPEWRNSPEFLNTIDWRSMAGTESE"
     gene            complement(17696..18955)
                     /locus_tag="VFQ79_18155"
     CDS             complement(17696..18955)
                     /locus_tag="VFQ79_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU0141652.1"
                     /translation="MIAALLVTAIVLSLLLLLCTFVQHLYLESLRLRARESAALVFFK
                     ETLEDRLGLKTDTGALAFSLVKHTLLLLLGIVFLAAAIPGVAFGLAILEAAVFSFLVM
                     IACAYLGPQLLYRRTGGTWLLPLLPLLKLLALIAKPFTILFGFLQSLAELGEPEAKRD
                     ENGRSNEDIDALISAGEEEGIFEKDDRRLIESVVAFGDKTVREVMTPRPNIVAISKDE
                     TLEGLRQLVINEQYSRIPVFENSIDQIIGFIHVRDMFEIEAEDRDKVTLAQLIRPIKS
                     VPETKPVHDLFREMQEDGTHMAVVIDEYGNTAGLATMEDLVEEVFGEIRDEHEPTRDI
                     EKIAENTYLVSGSFDLDHLYDLLHFRPEEETESTTVGGLVTEWLGRVPQPGEAVEREG
                     IRLEVVAGNEFRVDRVRISKSPGTVNA"
     gene            complement(18952..19401)
                     /gene="ybeY"
                     /locus_tag="VFQ79_18160"
     CDS             complement(18952..19401)
                     /gene="ybeY"
                     /locus_tag="VFQ79_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU0141653.1"
                     /translation="MSDTDDGPVLFRRSVVGLPRKSIRTFAERLEAEVAGGRRFECLL
                     TNDRELRRLNRDFLARDYPTDVLSFPSPDRAEYLGEIAISVERAAEQARDFGHGVDQE
                     IGILMLHGLLHLLGMDHVKDRGRMARAEARWRKKLDLPPALIERSRS"
     gene            complement(19415..20464)
                     /locus_tag="VFQ79_18165"
     CDS             complement(19415..20464)
                     /locus_tag="VFQ79_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU0141654.1"
                     /translation="MKLSVQISRGIESLFGTRDENIRLLESGLGVQAVLVDDTLEIEG
                     DEPNAIRAASILEDYAGLVKEGHVFSNGDLNSYLRVVTGDPEVTLRGLVVSGKQRNFG
                     KKALAPKTINQRRYLEAIERNDLVFGIGPAGTGKTYLAVAMGVSALLSKRVTRIILTR
                     PAVEAGEKLGFLPGTLQEKVDPYLRPLYDALYDMLEADKVEKLLERNVIEVAPIAFMR
                     GRTLNDSFIILDEAQNSTPEQMKMILTRQGFNSKMVVNGDLTQIDLPPGKRSGLLDAA
                     DVLRGVEGISFVQFDERDVVRHTLVQKIVKAYERYYENSGAGRQLSLKLGDGQAETPL
                     VPMIDGVSSGVVPAA"
     gene            20701..21192
                     /locus_tag="VFQ79_18170"
     CDS             20701..21192
                     /locus_tag="VFQ79_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0141655.1"
                     /translation="MPLSDATRIRKLYVNGREASIDADPNRTLLSVLRNDLDLTGAKY
                     GCGEGLCGACTVLVDGAAVRSCTTPVKAVAGKRITTIEGLAQQDRLHAVQQAFLDAGA
                     FQCGYCTPGMILSAVALLRENPTPGEAEIKDSLEGNICRCGTYPRIVAAVRLAATGKA
                     GGQ"
     gene            21189..23285
                     /locus_tag="VFQ79_18175"
     CDS             21189..23285
                     /locus_tag="VFQ79_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0141656.1"
                     /translation="MTTQPCFDVERYELAEDAAYWFPLSRREFIQTFGAGVVVLALAG
                     STDAQESAPREVGGWVHIGADGSISVFTGKTEVGQNIRTSLSQAVAEELRVSPEAIRM
                     VMADTDRVPFDMGTFGSRTTPTMAPQLRKAAATAREFLLDKAAKEWGTGRETLVSSNG
                     SVRDPASGRSATYGQLAKDEAVLKAVLTDTPFTAAEEWQVLGKSILKVNGRSFVTGSH
                     RFTTDVAMPEMAVGKVLRPPSIGATLVSVDSSAVPKADATVVVEKNFAGVAAGSEEAA
                     ERALAALRPQWSTTAQPSDSELFQLLKASGTPPPESTKGSIEEGMAAADTRISAAYTV
                     AYIAHAPLEPRAAVAQWENGKLTVHTGTQRPFGVKQELARAFGIPESRVRVIVPDTGS
                     GYGGKHTGEVAVEAARLAKAAGKPVKLVWTREEEFKWAYFRPAGLIETASGTRKDGLL
                     TAWEFRNYNSGGAGIQTMYEVPHQRVHFVRADSPLRQGSYRALASTANHFAREVHMDE
                     IAVALGLDPLEFRLKNLKDPRLRAVFEAAAEAFGWGRVKAGNGRGSGMGGGLEKGGYV
                     ATFAEVSSGKDLRSVKVERVVTAFECGAVVNPEHLKNQVEGALVMGLGGALFEAVRFE
                     NGRVLNARFSRYRVPRFSDLPRIETVLLDRKDLRSAGAGECPIVGIAPAIANALFRAT
                     GKRLRSLPLLPQEAAG"
     gene            23398..24549
                     /locus_tag="VFQ79_18180"
     CDS             23398..24549
                     /locus_tag="VFQ79_18180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141657.1"
                     /translation="MKAIMRQAAMMAVSGLLLTGLSWAQTSSLAGAVKGEDGKGLRDA
                     WVKIERKDIKGNYKVKTNKKGEYFHAGLPLGVYKITLEVGGRDVDQVDNVRTRLGDPT
                     EINFDLQQLKARRDAMQQAAETGQLTQEQMREMSPEQRAAMEKQLKERQQQLSKSKEL
                     NAAFNEGMEALKTKNFEVAVQGFTKASELDPKQHVVWGNMAEAYMGLAGTKTGAEQEA
                     AFTKAMEAYGKALELNPGDAAYHNNYALALARQKKFDEAQAELEKAAQLNPPGAGQYY
                     YNLGAVLVNIGQIEPAGEAFKKAIEADPNYANAQYQYGVYLVSKATTTPDGKIVPPPG
                     TKEAFEKYLQLDPSGPFADAAKGMLSSMESTVQTEYTNPDAKKKGGKKK"
BASE COUNT         5256 a         7122 c         7384 g         4899 t
ORIGIN      
        1 tcaagctagc cataattgcc cctacatcac tggtgttcgg cgtgtgggac tcgcgatcaa
       61 ctcaagtcaa gcagcagcgt gtgttcaggg ctaacatccg ggccacaaat gttcgggaag
      121 tgtcgcgttc agcccaatac acgcctgcga ccgcctatgt ggatgtcggt gcaatcagcg
      181 aagacatcga gaaaaagagc ggagatgaaa gcaatctatc gcaggagggc atggttcatg
      241 cgctcagcac tcagacagta ggcggggtca tgctgaccgg tgagagcaaa ctggtccgaa
      301 ctgtgaatgt caaccttgag gcgattcgag cacttcgggg taccgtcccc tcgcctacgt
      361 cagtccagac acagccggag actggcggcg agcagcgatc agcgggcacg gcggagtcgg
      421 cgcaacctat cgacggcacc gaggcgctgc aacgatacat tctcggcttg gctctggtcg
      481 ctgcgacgca cgagccggaa ctcaatcttc gcgaagggtg tcatctgaga ttcaaagatg
      541 gggcaacgca ttccataaag ctcatttatc gctccaaacc tgggatggag caagaactcc
      601 cggaggatgt gttgagtttt gcggaaacgg ccaagtcgaa cttcttcaag ctcgcaggca
      661 ttgacgaaga gacgatgcac cgtcgggacg ccgtgcttga aacgaatatt gctgagagat
      721 tcctctccta tccacagggc gaccgtgaca agatacgcaa gctagggccg atcacgcaag
      781 cgaccctcga ccgttttaag gcactgggca acgatccgtt caagacggtc aaagatgagt
      841 tgaaggccat gtcaaaggat ttgggcaaaa aacctcgaaa gaacgcgccg ccggttaaga
      901 aaatcgaggc ctttagaaag gttgcagagt tgctggatca actcgcagag aatgaagcgc
      961 ttcctgaagg cgtggccgat cttgcttccg aactcgctaa gacagcccgc gagcacgaca
     1021 actcgcatga cgcactcaaa catatcgaag aagagatcaa gaactacaag aaggcacaga
     1081 aggaaagtac gccaacaggt tctaacacgt aggaggcgga atgactttcc tccgcgtcac
     1141 agtctggttc ttcgacgatc gctaccacgg cctgctggat cctaccggac ctgcggaatg
     1201 gccgccgtca ccgtttcggt tgttctgcgc gctggttgct ggagtcgctc gccgaggcga
     1261 actcgaaagg gagaccggca gagtgctggc gtggcttcag aatctggagc cgcctatcat
     1321 catcgcgcca aggtcgaaaa tggggcgggc gatcaggaga tatgtgccga acaacgacgg
     1381 cgataacccc aggaagctcg accggaaaga caggttgaca gaaaagctta caataccaac
     1441 gctaatgtcg gtgaatccgg atgagatgcc aaaggtgcac tatctgtgga ctatcaagga
     1501 caaaacggct gtgccaatgg atcgcattcg ccacgcggcc cacagtctca cggcgcttgg
     1561 ttggggggtg gacatcgcat ttgccgatgc aagtaaggcc agcgaagcag acattcaagc
     1621 acttcaaggt gttcgatggc accctaagcc cggcgtacgg cgtgaccaag gaatgctccg
     1681 tgtgcccacg gcccatagca attttcaaga atgcacgctt tgcgacttga agcattgcca
     1741 tcagaccgcc acggagcgga tcgagcacgg taaaccactt cacacggtgg acaaaccacg
     1801 ggtattcgag cgagtgttct acgccagtgt cgagcagccg atcggccgtc cctgccgtgt
     1861 ctttgagttg cggaatgttg atggtgaatc cgaacggtac cctcagcgca aactcgtcca
     1921 tctggccggt atggttcggc atcttgcggc ccaagcgatg acgaaagacc cgccgcacgg
     1981 cgttggggag gattgggccg atacgttcgt cgctggtcat tggcggcgtg gtgagccggc
     2041 atcattgaaa aggaacgagt gcgtcgatga tgcagatcgc cgccagcaag gcgaagaact
     2101 cacgaccgtt gataagcatc ggcagctatc ctatctgccg cttccgtcaa tcggacgtgt
     2161 acacaccgat ccgggcgtgc ggcgaatcat gatcaccgcg ccggtgggcg acgacgcatg
     2221 gcttgactac gtagcacggc atctggcggg gcagatgtta aaacctttgc ggggcgatga
     2281 gtttggaggc gatggtccac ctctgcttgt accggtacgg catgatgaca tcgcgcgcct
     2341 ctacacgaag caggcaaaca tttgggccag tgtcaccccg gtgatcctgc ccgggcacga
     2401 tgaccacaag cccgaaaaaa ctcataagct cattgaaaga gcacttgctc aatccggtat
     2461 cgagcagccc tgtgagttcg agtggagtgc gttttcgcgg ttcccaaaat cactttccgc
     2521 ccataagtac gacaagaaca agaagccgac aggctacctt cgaccggatt acctgaacag
     2581 ccagaccgcg gtccatctga cgcttcgatt tagcaacggc ctgagcgccc ctggcccgct
     2641 ggtcatcggt gccgggcgcc acatcggcct aggtctgatg gccccacgcg aaaattggga
     2701 ttgaccatgt ccggttccac attgcctgct gcgacgtctt ggctagattc cgcttccctc
     2761 cgcgaaccgg tcgtcccttc gaatgatgcc gcgggtttca agcaggcggc agagttgggc
     2821 ggcggcttcc tcgtcgcggg gcgagagttc agtggggtcc acttcgatga acttgtctgc
     2881 gcccacaact tcgcgcgcgc gggcggtttc ctcgggcgca ttcgaggaga cggaacagat
     2941 ggcgatgaat ccgccgtggt tgctgaatct ggcgagttcc gaaacgaggg ggctttcaaa
     3001 gtcgtccgta attaccttta ccagacagtc gcgatcgaac agcattcgtt cgaggtggta
     3061 cgccgcttcc ttgcgcgcag taatccagag cgtagcgggg cggtggcccg cgcgggcata
     3121 gcgttccgcc ggggttacgc ggctcgagcc ggactcgagc gccgaaatgg ctgctttcgc
     3181 cgcttcgtcg gccggtgtcc ttcgcgatgt atcctcaatc atgccggcgg cgaccgtagc
     3241 gttcgtcacc ggatcgatca ggacaaagct cccggtagac cggttgcggc ggtaagagtc
     3301 aaagaacagc ggcttgtgag tttctatcgc cactcttccg atctcgttca ggtgcaggac
     3361 ggaggccggc gatttttcga gcgtattcac atccaccgtc tccagcaccg tcctcacggt
     3421 tccggatgtc tgttgcgtcg tgtgcttcaa cacgtagggc cgccccggct ccagcgggtc
     3481 ggcgttcatc cagactacgc tcgcgttgaa ccgccgcgac gcgtgcggca tggagtcggg
     3541 cgaaaccagc atatcgcccc ggctgatgtc gatctcgtct tccaggcaca ccgtaatcga
     3601 catcggggga aaagcctccc gcagttcgcc atcgtaggta acaatcgatt tcacgcggct
     3661 cgttcggccc gacggcagca ccatcagccg gtctccaggc cggagaaccc ccgaggccac
     3721 ctgtccggca tacccgcgga aatccaggtt aggacggatt acatactgta cggggaggcg
     3781 aaactgatcg agattgcgcg ccgccgtcgc gggaacggtc tccaggtatt cgaggagcgc
     3841 cggaccgctg taccacgggg tacgctcgct gcggccgacg acattgtcgc catccagggc
     3901 gctaatggga ataaagtcca ggtgcggaat ctcgagcggt gcggcgaaag cctgaaagtc
     3961 cgagcggatc gtgagaaaaa tctgctcgtc gaaatccacc aggtccatct tattcaccgc
     4021 tacgacgatg cgtggaattc ccagcagggc ggcgatgtag gcatgccggc gcgactgcgg
     4081 caggacaccc ttgcgggcat cgacaagaac tacggctaaa tccgccgtag aggctcccgt
     4141 cgccatgttg cgcgtatact gctcgtgtcc cggagtgtcc gcaataatga acttgcgccg
     4201 gggcgtggaa aagtagcggt aggcgacgtc aatggtgatc ccctgctccc gttcggcacg
     4261 gagcccgtcg gtcagaagcg agaaatcgat agggcccgcg ttacggttca ccgacgcttt
     4321 ttgcaccgag gcaagctggt cttcgtacac ccccttggag tcgtacagca gacgcccgat
     4381 cagcgtcgat ttaccgtcgt cgacgctgcc ggcggtggtg aaacgcagca gttctttggt
     4441 ttcgttttgc cgtagaaacg cttcgatgcc ctcttctgcc gtcatcggag ccgttactgc
     4501 catcagaaat agccctcgcg cttcttaatc tccatcgagc cttcctggtc gtggtcaata
     4561 atgcggttct cccgctccga ccgccggaac agcatcagtt cttcgatgat cttcggaacc
     4621 gtgtccgcct ccgaccggat ggcacccgtg caaggagtgc aaccaaggga tcgcatacgg
     4681 cacataacca tttgcggctt ctcaccggga agtaagggga tgtcgtgctc cagggggatg
     4741 aggctatcgc cgcgcaccac catcggcctc tctttggcaa agtacaacgg cacgatagga
     4801 atcttctcca ggtgaatgta ttgccagacg tcgagctccg tccagtttga cagagggaat
     4861 acgcggatgc tctccccctt gtcaatgcgg ctattgaaca gattccacag ttcgggacgc
     4921 tgattcttcg ggtcccactg tccataggca tcgcgaaaag agtaaacgcg ctccttcgcg
     4981 cgcgattttt cttcatcgcg ccgcgcgccc ccgaatgccg catcaaaccc gccctgggcg
     5041 atcgcatcga gcagtgcccg tgtctttagc agcccgcaac atttctgggt acccagccgg
     5101 aacggattcg ccccctcttc aatagcggcg gtgttggtgt gaacgatcag ccgggctccg
     5161 atgcgggccg tgaaagagtc gcggaaagcg atcatttccc gaaacttgta cgtggtgtcg
     5221 acgtgtaaca acggaaacgg aataggaccg ggataaaacg ctttctgcgc cagccgcacc
     5281 atgacggaag agtctttccc gatggaatac agcataaccg gcttctggaa ctccgctgcc
     5341 acttcccgca ggatatgtat gctttccgcc tcgagcgcac gcaaatgggt cagcgtcgga
     5401 agctgcataa aatcaatcat accattcttg atagagattt cgaagattca ccgaggctcg
     5461 ggtttaaggg acctgtaaca caaaccggtg caggtggcgc gtgtgcaact ggaacgcctg
     5521 ataaagcttc tccttcccat agcgtacatc cggtgtgtag tgatagctcc acgattcgtt
     5581 atcgagaagg agcgggatgg aggtgcctgc cggagtggcg ggcgccaggc ggatgaggct
     5641 cgtacgctct tttgcgccgg tccattccaa ctgcttctgc tcctggatat cgccggtctc
     5701 gtcaacataa ttggagtctg tgtagatctt cgttcgatac cagttgtttt tgtcgaaagg
     5761 gtccatgccc tgtttcatgc ggacccagat cgtggccgtc tcgccgggct ccagaacgcc
     5821 atttccgttt ccgctgcctt cggtcacggt tcgctgaacg gacgcgcccc caccctgatt
     5881 tcctttctgg tggaaaacat tgaatgtaaa cgtgcgtccg tccaggacct caaccgcgtc
     5941 ggggttgggg atcgtctccg gcgcaatcag cacgtcgata ttttcctgcg tactctgcca
     6001 gccgtcgtac gtcagccgga gttccaatcg cgcgtgcgtg aaataccccg cgcccgccac
     6061 aaagcgcact ttcatgtgcg aggagagatc ggccgcttgt cccggctcga ttttcggaat
     6121 atccccactg ccggagagaa cctgtaccgt cggatactgc gaactcagtg ccgccttgac
     6181 agcggtcatt ggcacgcttc gcggattgta aatgcgaatc ggaagtgcga tttcttcgcc
     6241 gggcggcagg cgtaacacct ccttgcgtgt aagcggcagc aggacggagg gctggctccc
     6301 ggtccccggc ccggcgaagc tgagctgatg cccttgtcca tcgacggtga acgacagcct
     6361 gcctttatcg tcgggaacaa tctgcctggt ttcggctgtt ccggtggtca ggttatagtc
     6421 cgtcaagtta taggaagcac cggtgcggta tagcggcgcg gtcagcacgc ggatgcgccg
     6481 ttccgccgga ggtccatcgg gcgcccagcg gcgcgtggta atccgcattc cgccctgata
     6541 cacggcttca aggtagacgg ttccgggctg ttcgccttcc gaagagaact catagcccca
     6601 cacctgaaac cggcgatagg ggttcgcatg gctccaaacc tccggaacgt tatcgagcac
     6661 actggagccg aaagcccgca tgtggaaccc gaatgtctcg ccgatggaag tagcccaatg
     6721 acggtgatac tcgtcgcggc ggtactcgta gtccacttcg cgcgccctgg cataggcctc
     6781 gtgggtctcc tcgtggtact ggctgatgta atcgccgctc gcgcgaatca gccgcaccat
     6841 ggtatggccg tggttgctta catgatcttt gggccgccag aggatgcgcc gccctttgtc
     6901 gccggtgtag aactccggcc ccgggttgaa ggcagaggca cttccgatca gatccggata
     6961 acgcgcgctg agccacaggc tcataaagcc acccatgctc aagccggacg tggcgcgatg
     7021 acggcggtcc gtgagcgtcc ggtacgtcga atcaatatgg gctaccagtt ctttgaagta
     7081 ctcgccaaag tcgtaatcgc ctccctcttc gcgaacgtcc cacggtgagc ccccgtagaa
     7141 accggtgtag tcccgcgcca cgtatccatc gacggcaacc acaatgcagc cgttcttcga
     7201 tacgaagccg gccatcttcg gaatcgtatc cttgccgtta tcgtactttt cgagcgtgta
     7261 ccggtcgcta tgcccgtgaa agtagtagat gacggggtac gctttgccgc tgccttggta
     7321 gttgggcggc aggaggatcc ggtaatgacg gtcctgtccg aacactttcg acggatgggt
     7381 ccggtcataa aacggctgtg ccgcccaggc ggcggtggca aacaacagaa acagaaaacc
     7441 tcgcagcatc ggcattaact cttctgtacc gggaacagcg tcgattccgt gcgggcatgc
     7501 gtcatgattt ggccgatctt tttcacaact tgtggattcg ccgccgcgag gtccttcatc
     7561 tcccccacat cctccacgac gttgtacaac tccacaggac catcggccga aaggcgcacg
     7621 cctttccatt catccatccg gacagcctgt ttgaagccgc cctcgtggaa ttcccaatac
     7681 agatactcgt gcgaggtctg ccggtttccg agcagcgccg gaagcacgga ggctccgtcg
     7741 atacccggcg gcgcgctcgc tcccgccagt tctgcggcgg taggaagcag gtcccaaaac
     7801 gcgaatactt gatcgctggt ccggcccgct ggaaccctgc ccggccagcg cgcgaagaac
     7861 ggaacacgga tgcctccatc gtaaagatcc cgcttgatgc cgcgcagcgg gccgttgcta
     7921 ttgaagaact ccggatcgtg gcctccttcg cggtgcgggc cgttgtcgct cgaaaatatg
     7981 aagagcgtat tgctgtcgag gcccttgcgc ttcaacgcgt cgaggatatc gcccgcctcg
     8041 cggtccagcc gggtgaccat cgctgcgaaa ttcttctcgg tttccggcca gtccctcccg
     8101 gagaacggcg cgttgtccgg tatctccata ccgttgccct gataacggcc cagctcgttg
     8161 tttgcatgcg ggatcgtgta cgccaggtac agaaaaaacg gcttcgcccg atcctgttcc
     8221 agaaaatcga gcgtgcggcg gtggatcaga tcgggggaat actgtttccg ctggttgaac
     8281 cagttctccg tcagaaacgc ttcgttctgg ttgtcccaca agtgactcgg gtagtaattg
     8341 tgggcgtgca actggttcag gtatccatag aaatgatcgt agcccctggt gttgggcaca
     8401 ctgccggtcg ccggaccacc caaaccccat ttgccgaaca gcgccgtctc ataaccggcc
     8461 ttctggagca gcgaagcgac ggtggtttcc tccgggcgca ggcccacctc gggtttcttg
     8521 ttgccgcgca cggtcgcacg gcctgaatga aggccggtca tcaggcagca gcgggacggc
     8581 gcgcagacgg tgcttccggc ataggcttgc gtgaaccggg ttccctcggc ggcaatacga
     8641 tccagattgg gtgtggagaa ccgcttctgt ccatagcaac tcaggtcgcc atagcccaga
     8701 tcatcggcca gcagaaaaat gatattcggt ttcgaagccg tggtttccgc gcccaaaagt
     8761 cccgccgcgg aggatgccag aaattcccgc cggttcatca taagctcttg cgcgactgcg
     8821 cgcgaactct tatcctaccc ccgaagcgat cgacccggcg tctactttag cgaagcaaca
     8881 tatttcgggt aattgcggtg gaccatgatg tggaaggtgg gctgccggaa ctcctcgaca
     8941 gcggagagga agcccttcaa gacgagtgat tgcaatacgc ggcgcatcca cttcacccct
     9001 tccggcttca tgaacttttt ggagatatcg agtgtggcgc ccgtcaggtg cgtcgagcgg
     9061 aaaaacccga cagcgggagc ggcgtttaca ttccgcaggc gcaacgaatt ctggtattcg
     9121 accgtgcgca ccatgctggt cacacggagc ctctggttgt gctttcggaa atactgtttg
     9181 ctcaggcgtt ccaggaacaa cttgctccag ggccgcagat actggtaatt gtgcggcacg
     9241 cggtgaaggt agtaacgacg cgtggaagag ggaacccgta ccagcaggcc ggccttcgcg
     9301 aaccgcttca ccatcgccac gttcttcata cgggacaggt cgtcgcgatc ggcccgctta
     9361 ttttggatca cctgcgaatt acgcgccgca cggagtgcca gcgcacctcc ggaggcagcc
     9421 attggaatag ccgcacagaa caccagtgca gccactaaca ctttggccat tagctacaga
     9481 ttaagaacga ttaacggtac gcgtcaagcg ctggcttggc tacttcggcc agtgccaaat
     9541 gccctcggtg ttgcaaagcc ttgaccagac accgactccg gttatgatct ggaagcagtg
     9601 gaggccgcga tcggtccgat tcgctgaatt gcggaagcgg agcgtgccaa agacttgccg
     9661 cgtgtacggc agggagcgcc cgtgatcggt ggaggctggt gaacagccca gcaccccaaa
     9721 acactgacaa acgtctcttt cggctcgctc aggattccga gattgactgg cggccattag
     9781 ctcaacccgc gaaacgtatg cggagccgca tgtatggtgg tgtcgcaggg aaagtgggcg
     9841 accgcctaca tatgcaaatt cttacgccgc aaagtctggt attaaatctc tttccttgag
     9901 caacgccagg agatttctat aggcattgag aagggctcca cgattagccc agttgttact
     9961 ttcgtcagtc actttggggt tgatgaatgt ccctgacggg ctaatgtacc aaggatctgt
    10021 gataattcct ttggtcatat cccaatcgtg gtcgaagacg agagtgaagg tttcgacaaa
    10081 gtggagcact gctgtttcta actcaggatc tcgaatcatc aagtggatta tggcgcaatt
    10141 cacggtggcg gtcgagctta cttcggaaga tctgcccttt gtctccggat accttctggg
    10201 tccgggaccg attaattcgg aaagtcgaaa aggaggatgc ggcagtgaaa ccacttctgc
    10261 caattgtgct gatcgtactc accgcgctgg caggtatggc ccaacaaggc cgggtagggc
    10321 gtggacaacc gccgccaaac cagggcagcg cactgcttca tatcactccg acgccctcgc
    10381 aaattttcag ttcgttgaag gacctggccg ccgcttcgct gctcatcgtg gaaggcacgg
    10441 tcaaggcaac gttgccttcc cgcgagacaa gtccgcgggc actggaaacg gatgccgtga
    10501 tcgccgttcg cagagttcta aaggggccag cggacgttcg cgaagtcgtg atatcacagc
    10561 gaggtggaag ccgtggaggg ctggttgtaa tgcccgctca atattccctg gtgcagccag
    10621 gtgagcacta tgtgctgttt cttacggaag acagccggcc tgatattccg gacgtgccgg
    10681 gtgtacgccg ctacctggtc acggggctat ggacgggact tttccgtttt caggaaggga
    10741 agatgcacac cgatgcgcgc aaggtagatc ctctgcgcgc aaagtacgaa ggcgtcagtg
    10801 cggaagaaat tatcgaagag gtcaaagcgt cgctatggta ataaacgttt caatcatttc
    10861 ttaagatcgt gtagcgcggc gcgcgcggca tagtatccac acatgccatg cactcctccg
    10921 ccgggcggtg tggaagcgga gcagagatag agtcctttga ccggcgtccg gtaaaggcgc
    10981 catgtgggcc gggccagcaa ctggcgccaa tccgccacgc cgccgttgat gtcgccgcca
    11041 accaggttgg ggtttccccg ttcgagctcg cgcggggact tcacgctcct ggccagaatg
    11101 cagtcgcgga aacccggcgc gaagcgctcc acctggttct cgattcggct cagcatatcg
    11161 gcgtccgatg catgcggcac gtggcaatag gcccaggcgg tgtgccgacc tgcgggcgca
    11221 cggtcccggt cgaagaggct cggctgcgcc agcagcacaa agggacgttc gcagaggccg
    11281 cccgcccagg ccgcccgttc ggacgcctcg atttcctgcg gcagtgcacc cagatgaacc
    11341 gtgatcgctt cggcgcagcg gggtgagcgc cagggaatgg gctcccttaa agcccaatcc
    11401 actttgaatg ctcccattcc atatcgataa cgttcgagcg agcggcggta gccggaaggc
    11461 aggctttcgc ccgcaatacg cagcaactgg cgtggcgtta catcgcatag gacgatcctt
    11521 gtggaaggca gttcgctaag gctgtcgacg cggttgccgg tgacaattct gccgccgaga
    11581 gaacgaagat atgccgcaag cgcatcggcg attttctgcg cgccgccgcg ggggaccggc
    11641 catccatagg cgtgcccgta gaggcccagg accagaccga acgcagcaga aggaatgcga
    11701 tcgagcggaa gcatggagtg cgcgctcagt ccgagaaaca acgcccgcgc tttcgttccc
    11761 tggaactggc gatgggccag accgcgtgcc gtgcgaaacg catccatgcc caagcgcgcc
    11821 agcgagccgg gataccggag gagatggaaa ggatgcagaa aggcagtctc gatgcggctc
    11881 caatgttcga gaacggaaga aaagaactcg ctgtaagctt gccggtctac cccaagggaa
    11941 gccgacgtca cggaaaccga gcgttccagc acgacgcact cgtccgcttc gagagggtgt
    12001 gccaaagcag cgtccggatt gatccattcg agcccgaact gtcccagtgg aagccggcga
    12061 aaaaaaggcg acgcgtatgc cagggggtgg acggccgaac agacgtcgtg cgtgtatccg
    12121 ggaagggtca actcggcgga ccggacacca ccacccacta cgtccgacgc ttcgtagacc
    12181 gagacgctca gccccgcgcg gacgagttca atggcggcgc tgaggccgtt aggaccggcg
    12241 ccaacgatga tggcatcgtc attcggcatt tttcaggccg tgcctgccgg aggaaacaac
    12301 cggagcaggg cggcatccaa tcagtgttcc actccccgcg tgtgatttgc cgggcaatgt
    12361 ggcacagaac gcaaggaaac ctgggaggac tcatgtcgaa ataccgtgta gtattcatgg
    12421 agcggacaga ccagtttggt gaaaacatcg agagcccggc cgaatacctg atgcctcaac
    12481 ttagcgatgg agtggtgatc gatacggaat ttgtgtcgcg caaccaaccc gaggcgcaac
    12541 attcacagga ggttctggac gaagacgaca gctttctgag catcggaacc gagatctgga
    12601 tgtacgagat cgccgacggc cgggagcagg aatttgaaga cgcggcgcgc aattcgcgca
    12661 tggtgatcga gctcgaaagg atggacgaca cggagatgat cggctcctga gccgccgatc
    12721 agttcgcgat cagcaactcg tgaaggcgtt ggacccgcgc acaaagggtc acgcgcgaac
    12781 ggttgccgat ccgttttgga gcaagctctt tcagaggctg gaaacataca gcttgtggaa
    12841 atggccgaac gatggctaga tggcaacaaa gccggagtgt accctcgatc tggatctcgt
    12901 aacgatgggc gacttgcacg ggtggcagag cccgataggc gtctcagtga ccttacataa
    12961 agcagacgaa gtccttgagg agattcgaga aacaaggaaa cgagtgcatc gggagatgta
    13021 gatcgctggt atacggtgat gagtatatca gcccggtcct gcggtcattc ggacggagga
    13081 tgcgcgggct actcgtaagc cgtggctgcc gtactgcggg gcccgtattt cgggagcggc
    13141 tggcccactt catacgcacc gaggtccggc gcgttcccca cgaagccgtc gttcacgcca
    13201 ggtaagtgga tgcccgcatc taccgccttg ccgtgcgggt tcaatttgaa attcagttcg
    13261 cttgcatgat acacggcttg cggtttggcg gcgtcaggcg cccgcagatc ttcgaagatg
    13321 ttgtaatcga cctcgatgcc atgagcctcc tgtcccgtta ctgttcgcaa ctctgccagc
    13381 gaaccgaatc gtttcgcact cccgcggttg agagtataat cccgcagggc gtcggcagcg
    13441 ggggatatcc agaggaattg atctttcgcc tgcgggttca ggcggtaacc gttgtagtcg
    13501 taagtggagt aggcagtagc gttggagaat cggagcacgg cgcggccggg agcatcggtg
    13561 ccgaggaaca ggttgttccg aaagtgcgca ttggagaaca ggtcgccggg cagaccttca
    13621 gcaatgatgg tgttgtgata gagcaccagt cctgacggct tcacgttgaa cttcagcccg
    13681 catcccgtag ggacgtggta cacgacgttg cggatatagt aggccggtcc gccgtatact
    13741 ggctgcgcgc tcagtccaca ctgtgcggcg ttgactccgc gattccggaa aatgcgaatg
    13801 ttgtgaacgc cgccatcagc ctcgatgaaa tcgtcggcca tcaggtgaat atcgttgttg
    13861 tagaagtcga tcgacgatgc tttccggtcc tcttccggat ccggagaccc atgcgtgcat
    13921 acggagatac cgtcgtgaaa ataggcgatg taattgtggg agactacatg gccagcgccg
    13981 tagaccttga ccgcatagta gctgttcagg aggttgcgtc cgtagatgcc ggggttgtac
    14041 cagcctctta accgatagcg atcgtcgcga ccaagcatga cattgtccga gatgtggaag
    14101 tcgtgggaac cggagtattg cgtcgtgacc ccgatgccga cgtcctcgat gcggcagctt
    14161 cgaacagtga gaccttttgc gccgatcaaa tcctttaagc cggcctggaa cgcgatgtcg
    14221 gcatttctaa tcgtgagtcc ttcaaaaatg tgataatcgg cgcccatgac gttgaacagc
    14281 tcgtgcgctc cgtcaccatc gaagatcgcc tcgccgtcat cagcgccgcg gatcacgatg
    14341 ggctgctcag gagtgccttt ggcggtcagc acgtaggtcc ctgtaaagtc cagcccgagc
    14401 ggatgcgagt attgcagccg gtcgcctttg taaaggcccg cgtgcaccag tatcgtgtcg
    14461 cccgggcgaa ccttgcgctc actcacgacg gcccagtccc cattgccgga gccgaaatac
    14521 gcctgcttca gtcctgtgaa tgccggttgc tctttggcgc cggtccagcc cggcggataa
    14581 acgtgcagga tgcggcctcc cgaagcggcc ttcggctccg cccgcgtcct gaccttcgca
    14641 gtttgtacag cggcgccatt gacgccatcg gggtcgcgca gttcaaagcg ggcttcatac
    14701 gccgtatcgg gctgtaggtc gaggatgctc ccggcgaaca tatcgggtac cgtgtagtcg
    14761 aggccgagat cgggtcgaat gaccttttcg ccgccgagac ggagcagcgg catgcccttc
    14821 tgccatgctt gctcgccaac gcgacgatag ctgacttcga cggaggcatt ccggttgtcg
    14881 tcaccggtga tctgccattc gaaaccgagg cagagcagcg tggggcgctc caccttgagc
    14941 cgcattgcgg taactgagtc ggcggcaaac agcgtcggac aactgagcaa gaggaagctg
    15001 gaaacaatgg ccggtccagc aagcaatgta tgctgcatgc caaaaagctt atcatcgtgt
    15061 gtcagacagg aaccgccatg ccgccttccg gcctgcaatg ccgcctcgtg tggtagaatc
    15121 ccggcgagga ttcatctaca gccatgaaca gcaggagaac tttcctgatc ggtaccggcg
    15181 ctgctgcgtc gcaggtactc gtacgcaaag cgggggcaaa cgatcgcgtc cgggtcgcgg
    15241 tgctcggagt gaacggccgc ggcagggatc acatagaagg gtttcaggga gtgcccggga
    15301 ccgaggtggc gctgctgtgc gaccccgata gcaagctcct gggcgaacgg gccgcccaat
    15361 tccaggaaaa gtacgggcga aaagtcgaga ccgcgaacga cttgagaaag gtattcgagc
    15421 ggaaggacat cgacgcggtg agtgtcgcca cacccaacca ctggcattcc ctcgccggga
    15481 tttgggcgtg ccaggccgga aaagacgcgt acgtggagaa accggcttcg cacaacgttt
    15541 tcgagggccg gaagctggtg gaagccgctc ataagtacaa gcggatcgta cagcacggag
    15601 ttcaattgcg cagctcggac gccattcagg aagccgtcca actgctgcga aaaggagtga
    15661 tcggcaaggt gtatatggcc cgcgggctgg tctatcgctg gcggccctcg atcggacata
    15721 agccaaccga accggtgccc tcctacctcg actacaatct ctggaccggc cccgcgcagc
    15781 agaggccctt ttcacgccgt ctggttcatt acaactggca ctggcattgg gactacggca
    15841 atggcgatgt cggcaaccag ggcattcacg aaaccgacat gtgcatgtgg ggactcgacg
    15901 tcggcctgcc gaagcggatt acggcgatgg gcggcaagtt cctgttcgac gacgacaagg
    15961 agacaccgga gattctcacc tccgtgtatc actaccccga agagaagaag atcatccaat
    16021 tcgaagtaag acactggtgt acaaacgaag agcacggcgt cggcgtaggg aacatctttt
    16081 acggctcgga aggctacatg ctgatcaggg gttataacac ctacgaggtg tacctcggtc
    16141 aaaaacggga gaagggaccg tccgggaaat cgggcggcga tcactacgcc aacttcatca
    16201 aagcagtccg cagcagaaaa acctcagacc agaacgggcc ggttgaaacg gcgcacctgt
    16261 catcggcgct cgcccatctc gggaacatct cgttccggct ggaacgtcaa ctgacgttcg
    16321 atccggccac cgagaagttc attggcgatt ccgaggcgaa ttcgatgctc acgcgaaact
    16381 accgcgcgcc ctttgtcgtg ccggaaaaag tctgattgga cgctgcgagc ggtacgggcc
    16441 gggcgcgttc aggaagtgcg taccggctcg atcttcaact cgttaataac gcgcttcacg
    16501 ccggggacct tcttggtcag tttttcggcc ttctgcttca gcttttcttt ttcaacggtc
    16561 ccgcgaaggg tgacgacacc ttctttgact tccactccaa gcgtgccgcc cttcacatcg
    16621 gggtcgttgg ccaggcggcg gcgcacctgg tcgtaaatgg cgtcatcgga aatttcgaat
    16681 ttctggctcg ccacggttgg ccaggcgagc gcaaacaaca cgcatatcag caagaaagac
    16741 agccgtcgta tttcactcac tttcagtacc tgccatcgac cgccagtcta tcgtattcag
    16801 gaattcgggg ctgttcctcc attccggccg cactttgaca aaaagctcca gaaacacctt
    16861 ccggccgaaa aagctttcca actcctgccg ggctaaagta cccacttcct ttagcatggc
    16921 gcctttcgag ccaatgataa tcgccttctg gccttcgcgc tccacataaa tggtggcggc
    16981 aatacgaagc aggttcccgg tctcctccca agtctccacc agcacggcga cagagtgcgg
    17041 aacctcctgc cgtgtcaact ccagtacctt ctcccgaatg atttcggcgg cgagaaaacg
    17101 ctccggctga tcggtaagat agtcgcccgg aaagagcgga gggccttccg gaagccccgc
    17161 gatcattgct ttgaccaggt cctccaaacc atctccagtc gcggccgaga tggggatgta
    17221 ttcggcgaaa tcgagcagcg tgcgcaactg ctcgatacgc ggcagaatct tgcgcttgtc
    17281 tttcaccttg tcgattttat tgaggagcag aaacaccggc gcctgccctt tccggacaag
    17341 atccagggcc ttctcctctt cgatggaatg aggccgcgag acatcgacca gaaacagaag
    17401 caggtcacga tcttccaccg ccgcgcgtac ggtctgcatc atcttgcggt tgaaaagact
    17461 gtcggacttg tgaatgccgg gagtatccag aaatacgatc tgggcatcgg gcaacgtgag
    17521 gacgccttga accgacgtgc gcgtggtttg cggcttgtcg gcgacgattg ccagcttggt
    17581 tcccaccagc gcgttcagca aggtggattt gccggcgttc ggaagtccca ggatagagac
    17641 aaaacccgag acatgcggct ttttctttga cggcgaaccg tccggactgc ggcgcttagg
    17701 catttaccgt ccctggcgat ttcgagatac gtacgcgatc cacgcggaat tcattgcccg
    17761 ccaccacctc caggcggatg ccttcacgct ccaccgcttc gccgggctgc ggcacccgcc
    17821 ccagccactc ggtaacaaga ccgccgaccg tggtggattc cgtttcctct tccggccgga
    17881 agtgcagcag gtcgtagaga tgatccaggt cgaagctgcc ggagacaaga taggtgtttt
    17941 ccgcgatctt ctcaatgtcc ctggtgggtt cgtgctcgtc gcggatctcg ccgaacacct
    18001 cctccaccag gtcttccatg gtggccagac ccgccgtgtt tccgtattcg tcgatgacga
    18061 cggccatgtg cgttccgtcc tcctgcattt cgcggaacag atcgtgaacc ggcttggttt
    18121 ccggcacgga tttgatgggc cgtatcagtt gcgccagggt caccttgtcg cggtcctccg
    18181 cctcgatctc gaacatgtcg cgcacgtgaa taaacccgat aatctggtcg atcgagttct
    18241 cgaaaacggg gatgcgcgag tattgttcgt tgataactaa ttgacggaga ccttccaggg
    18301 tctcgtcctt cgagatcgcc acgatgttcg gccggggagt catgacttcg cgaacggtct
    18361 tatctccgaa ggccacgaca gactcaatga ggcggcggtc gtccttttca aaaatccctt
    18421 cctcctcgcc cgccgaaatc agcgcgtcga tatcttcgtt cgacctgccg ttctcatccc
    18481 gtttggcctc cggctcgccc aactcggcca gcgattgcag gaatccgaag aggattgtaa
    18541 aaggcttcgc gatcagcgcg agcagcttga gcagcggcag caggggcagc aaccaggtcc
    18601 caccggttct ccggtatagc aattgcggtc cgagataggc gcacgcgatc attacgagga
    18661 aagagaacac ggcggcttcg aggatggcca ggccgaaagc gacgcccggt atcgccgctg
    18721 ccaggaagac gatcccaagc agcagtaaca acgtatgctt caccagcgag aaggcgagcg
    18781 caccggtgtc cgtcttgaga ccgagccggt cttcgagcgt ctccttgaag aaaaccaacg
    18841 cagccgactc tcttgcacga aggcgcagcg actccaggta aagatgctga acgaaagtac
    18901 agagcaacag caggagagag agcacgatag cggtaaccag cagagccgct atcatgaccg
    18961 gctccgttca atcagggccg gaggaaggtc cagtttcttg cgccaccggg cctcggcgcg
    19021 ggccatccgc ccccggtctt tcacgtggtc catgccgagc agatggagaa ggccatgcaa
    19081 catcaggatt ccgatttcct gatccacacc atgaccgaag tcgcgcgcct gctccgccgc
    19141 gcgttccacg gagatggcga tctcgccgag atattcagcc ctgtccgggg atgggaacga
    19201 cagaacgtcg gtggggtaat ccctcgcgag aaaatcgcga ttcagccgcc tcaactcgcg
    19261 gtcattggtg agcaggcatt caaaccgccg gcctcctgcg acttcggctt ccagccgttc
    19321 cgcaaatgtc cgaatgcttt tccgcggcag gcccacaaca ctacggcgga acagaaccgg
    19381 gccatcatcg gtatcagaca ttggcactca tccgtcaggc ggcgggaacc acgccggaag
    19441 agacgccgtc gatcataggg acaagcggcg tctccgcctg tccgtcgccg agttttagag
    19501 acaactgccg gccggcaccg ctgttttcgt agtaccgctc ataggctttg acgatcttct
    19561 gaaccagcgt gtggcggacg acgtcgcgct catcgaactg gacgaaactg atgccctcaa
    19621 ccccgcgcag cacatcggcc gcgtccagca ggccgctgcg ttttccgggc ggcaggtcga
    19681 tctgggtcag gtcgccattt accaccatct tcgaattgaa cccctgccgg gtcagaatca
    19741 tcttcatctg ctccggtgtg ctgttctggg cttcgtcgag gatgataaag ctatcgttta
    19801 aggtacgtcc gcgcatgaag gcgatcggag ctacctcgat gacattgcgt tccagcagct
    19861 tctccacttt gtctgcttcc agcatgtcgt agagagcatc atagagcggc ctcaggtagg
    19921 ggtcgacttt ctcctgcagc gtacctggca gaaatccaag cttctcaccg gcttcgacag
    19981 cgggcctggt aagaataatt cgcgttactc gtttacttag tagcgccgat acacccattg
    20041 cgacagcgag ataggtctta cccgtgccgg cgggtccgat gccgaataca agatcgttcc
    20101 gttcgatcgc ttcgagatag cggcgctgat tgatggtctt tggcgcaagc gctttcttac
    20161 cgaaattacg ctgcttcccg ctgaccacaa gtccgcggag tgtaacttcc gggtccccgg
    20221 tcacaacacg aaggtaactg ttgagatcgc cgtttgagaa aacgtgcccc tcttttacga
    20281 gaccggcgta atcttccaga atgctggcgg cacggattgc gttcggctcg tcaccttcga
    20341 tttcgagcgt gtcgtccacc agcactgcct gcactcctag accggattcg agcaggcgga
    20401 tattttcgtc gcgggtccca aaaagggatt cgatgccgcg gctaatctgc acgctaagtt
    20461 tcattgatgc ttatatgatt tgacctggct gacatttgca gatgcaaaca gagatctgga
    20521 cggtaaaatg ggaacctgcc gagccggaga ttgcaagtga aatttctgaa gacgatatcc
    20581 gctggcgcgg accagtctcg aacatcctgt attagtataa catgagcgaa ccttcagaaa
    20641 ccatgttttt tggcagccat ctaacttgaa taaagcccgt tcctgcggta ggaatgctat
    20701 atgcctcttt ccgacgcaac ccgaatccgg aaactgtacg tcaacgggcg ggaagcctcg
    20761 attgacgccg acccgaaccg gacactgcta agcgttctgc ggaatgacct tgacttgacc
    20821 ggtgcgaagt atggctgtgg tgagggatta tgcggagcct gcacggtcct ggtggacggg
    20881 gcagccgtcc gctcctgcac cacacccgtc aaagcggtgg ccggaaaacg aatcaccaca
    20941 attgaagggc tggcgcagca ggatcgtctc cacgcggtgc agcaggcttt cctcgacgcg
    21001 ggtgcgtttc agtgcggtta ctgtacacct gggatgatcc tgtccgcggt cgctttgctg
    21061 cgggaaaatc caacaccagg cgaagcagag atcaaggact ccctcgaagg aaacatctgc
    21121 cgatgcggta cctatccccg aatcgtagcg gcagtccgcc tggcggcaac gggtaaggcg
    21181 ggcgggcaat gaccactcaa ccctgcttcg atgtcgaacg atacgagttg gcggaagacg
    21241 ccgcctactg gttcccgctg agccggcggg agttcatcca gacgttcggt gcgggcgtcg
    21301 ttgtcctggc gcttgccggt tccactgacg cgcaggaatc cgcgccgcgc gaagtgggcg
    21361 gatgggttca catcggcgcg gatggctcca tttccgtttt caccgggaag acggaagtag
    21421 gccagaatat tcgaacatct ctcagccagg cggtggccga ggaattgagg gtctcgccgg
    21481 aagcgatccg gatggttatg gccgacaccg accgggttcc attcgatatg ggcacgttcg
    21541 gcagccggac tactcccacc atggccccgc agcttcgcaa ggcggccgca acagcacgcg
    21601 aatttctact cgacaaagct gccaaggaat ggggtaccgg ccgggaaaca cttgtaagct
    21661 ctaatgggtc ggtgcgcgat ccggcgagcg gcagatccgc cacctacgga cagcttgcga
    21721 aagacgaagc cgtactaaaa gccgtcctta ccgatacgcc ttttaccgct gcggaagaat
    21781 ggcaggtgct cggaaagtcg attctgaaag taaacggccg ctcgtttgta acggggagcc
    21841 atcgcttcac gaccgatgtc gcgatgccgg agatggcggt gggaaaggtt ttgcgcccgc
    21901 cctccattgg cgcgacgctc gtgtcggtgg attcgagcgc ggttcccaaa gcggatgcga
    21961 ccgtcgtagt agagaaaaat tttgccggtg tcgccgccgg gagtgaagaa gcggccgagc
    22021 gcgcgctggc cgccttgcgc ccacaatgga gcaccacggc acagccttcg gacagcgaac
    22081 tttttcaact gctgaaggca agcggcacgc caccgccgga atctacgaaa ggctccatcg
    22141 aggagggcat ggcggcggcg gataccagaa tttccgccgc ctacacggtg gcctatatcg
    22201 cacacgctcc gctcgaaccg cgggcggcgg tcgcccagtg ggaaaacggt aagctgaccg
    22261 ttcataccgg cacccagagg cctttcggag ttaaacagga actggcgcgc gctttcggga
    22321 ttccggagtc gcgcgtgcgt gtgatcgtcc ccgacacggg ctcgggctac ggcgggaaac
    22381 ataccggtga ggtggcggtc gaagccgccc ggctggcgaa ggcggcggga aaaccggtca
    22441 agctggtctg gacgcgggaa gaggaattca agtgggccta tttccggccc gccggcctca
    22501 ttgagacggc gagcggaacc cgcaaagacg gcctgctgac ggcatgggag tttcgcaact
    22561 acaattcggg cggcgcgggc atccaaacca tgtacgaggt tcctcaccag cgcgtccatt
    22621 ttgtgcgcgc ggattcgcct cttcgccaag gctcttaccg cgccctcgcc tccacggcca
    22681 atcatttcgc gcgcgaggtc cacatggacg aaatcgccgt cgcgcttggc ctggatcccc
    22741 tggagttccg gctgaagaac ctgaaagacc cacgcctgcg ggcggtgttc gaggctgccg
    22801 ccgaggcctt cggctggggc cgcgtgaagg cgggcaacgg gcgtggatcc ggcatggggg
    22861 gcgggcttga gaaaggaggc tatgtcgcaa cgttcgccga agtatccagc ggcaaagatt
    22921 tgcgtagtgt caaagtcgag cgtgtggtca ccgccttcga atgcggtgcg gtggtgaacc
    22981 cggaacattt gaagaaccag gttgaaggtg cgctcgtcat gggtttggga ggagcgcttt
    23041 ttgaggcggt acggtttgaa aacgggcgcg ttctgaatgc gaggttttcg cgctatcgcg
    23101 tgccgcggtt cagcgacctg ccgaggatcg aaacagttct cctcgaccgg aaggacctgc
    23161 gctcggcggg cgccggcgag tgtcccatcg tgggcatcgc gcccgccatc gcaaacgcac
    23221 tgtttcgggc aaccgggaaa cggcttcgca gcctgccgct gttgccgcaa gaggctgccg
    23281 gctagcagat ttttcgtccg gccggtggag ggtttcaggt gtgagtatcc cgtttgaaac
    23341 ttaactttcg tagtatcctg aaagtgtaag cttcactcga gttggaggtg tatttctatg
    23401 aaggcaatca tgcgccaggc agccatgatg gccgtttccg gtctcttgtt gaccggtttg
    23461 tcgtgggccc aaacctcgtc cctcgcgggc gcggtgaagg gagaagacgg caagggcctc
    23521 agggacgcct gggttaaaat cgaacgcaaa gatatcaagg gcaattacaa ggttaagacc
    23581 aacaaaaagg gcgagtactt tcacgccggc ttgcccctcg gcgtatacaa gatcacgctc
    23641 gaagtcggcg gccgcgatgt ggatcaggta gacaatgtcc gtacgcggtt gggagatccg
    23701 acagagatta atttcgacct ccagcagttg aaagcgaggc gcgatgcgat gcagcaagcg
    23761 gctgaaaccg gccagttgac gcaggagcag atgcgcgaaa tgtcgccgga gcagcgggcg
    23821 gcaatggaaa agcagctcaa agagcgccaa cagcagcttt ccaagagcaa ggaattgaac
    23881 gcggcattta atgaaggcat ggaagccctg aaaacaaaga atttcgaagt tgcggttcag
    23941 gggttcacga aggcttccga gctggacccg aaacagcatg tggtttgggg taatatggcc
    24001 gaggcctata tgggtctggc cggcacgaag acgggagccg agcaggaagc cgccttcacg
    24061 aaggcgatgg aagcatacgg caaggctctc gagctgaacc cgggcgacgc cgcctaccat
    24121 aacaactatg cgctggcgct ggcgcggcag aagaagttcg acgaggcgca ggcggaactg
    24181 gaaaaggccg cgcagttgaa ccctccgggc gcgggccaat actactataa tttgggtgcg
    24241 gtgctggtca atataggcca aattgagccc gccggtgagg ctttcaagaa ggcgatcgag
    24301 gccgacccga actacgccaa tgcgcagtac cagtatggcg tgtacctggt ttcaaaggcg
    24361 accactaccc cggacggtaa aatcgtgccc ccgcccggga ccaaggaagc tttcgagaag
    24421 tacctccaac tcgacccaag tggacctttc gccgatgcag ccaaagggat gttgtcttcc
    24481 atggaatcaa cggtccagac cgaatacacc aatccggacg ccaagaaaaa aggcggcaag
    24541 aagaagtaag tccggtgttc tctcgcgggg cgccgctccc ggtgccccct atgcgatatg
    24601 attcgtttcg tcaccatcgc gctgatcgca attgtcctca tcactcttct gagatttact
    24661 c
//
LOCUS       DASTMK010000422         5848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_212678, whole genome shotgun sequence.
ACCESSION   DASTMK010000422 DASTMK010000000
VERSION     DASTMK010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212678"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFQ79_18185"
     CDS             <1..685
                     /locus_tag="VFQ79_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141658.1"
                     /translation="QTSDWRSRYQALQLRVQRSYSAGATILVAYNYNQERNEAFFNDP
                     QHYVNQVFWLGSNNARHRLTLAGTYDFPIGKGRKIGGGMHPVLNAVAGGWQVSGIYTY
                     RSGEYLRFGGMDLIGDPVIDNPGPGGWFNTDAFRIQTAFTPRMNPYQFSDVTGPIFWN
                     LDGTLSKNFPIMERYTLEFRFEAYNLTNSLMWANPNMTVGNAQFGRSGAQANQNRGRE
                     MQYTLRFIF"
     gene            complement(710..1570)
                     /locus_tag="VFQ79_18190"
     CDS             complement(710..1570)
                     /locus_tag="VFQ79_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU0141659.1"
                     /translation="MRWTPGGRSPNLEDRRGAGGGGLRLGRGGAGLGLGGLLLMLVLS
                     FIFGQDFLSLLSTSETSVPYTRSDPIQDANEEPMVQFVSFVLDDLQKTWRQILPAAGR
                     NYRDAKLVLFRDTVQSGCGFAEAQTGPFYCPADEKVYIDLSFFAELHQRFGAPGDFAQ
                     AYVLAHEIGHHVQHVLGIDRQVRRAQDAGVASANELSVRTELQADCLAGVWGHSTAAR
                     DILEKGDVEEGLNAAAAIGDDRLQRMGRGHVSPETFTHGSSEQRAGWFRRGFETGDVS
                     ACDTFGAGAR"
     gene            complement(1629..4841)
                     /locus_tag="VFQ79_18195"
     CDS             complement(1629..4841)
                     /locus_tag="VFQ79_18195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141660.1"
                     /translation="MTARSVLSLYLLVICSGTAVSQTYYGTIRGTITDATGAITPGVK
                     VTVTNIDTNISQDVESNSVGNYVAPNLIPGRYRVTAEPPGFKRFVVEDIELTATADRR
                     VDVRLEVGQVTESVTVEGGAQLIETERATISDTKSNYVFTNMVINSNYRSIWRMLVLS
                     PGVSGSTFAGNGQGRNTTYSIDGIPIKDGWTGSSFGPALTYLDSYREYRVDLVSVNAS
                     GGTSSNVAVVSESGTNQLHGEAWLHYNAIGFGARDFFAPARPHGPPIFRPNIKIGGPL
                     YLGKLYNGRDRTFWHFSWQGLRGSQTPTVANFVVPTAAFRAGDFASLGTPIIDPSTGS
                     AFPNNRIPESRISPVAKYYQDTFYPLPNSGTDRYNNVAVFPNTSDQYTARGDHKISNA
                     NSFFGRFMYQHYEFETWDGGANPNIGNYSQWRDQYHVVLSDTHVISPSMLNEFRVGYA
                     RDQSEYGGPNPGLPIVQAAGLQLADLQDVPGLPRMNITGFQPIYQGDMNGWMWSNFHV
                     QETFHITRGKHNFRVGLEVGKYNGKQYATSPSAVFGIFGFNGRFSGDPYADFLLGLMD
                     TSDRRTSVGPVYPHRLNWELFFTDDFKITPRFSLNYGLRYSLLDPGMIEQNLLANFVP
                     AYNALVVPDEAARARVHPGFPASIPIVTANSVGLGAKLLNLDKNNFAPRVGFAWRPEF
                     WNNFVIRGGAGVYYVAMQPYISDGGGAPYELRETFTNAISGGVPAFSFPRPFPGTAYV
                     LGGTGASGMSPDFRTPYSTQYNVTMEKEAFDMGFSVSYMSTLSRKNVWSRNLNQPPAD
                     TRPYAEKLPLVPFPYLFSASYTENGGSHSYHAGVIKAERQFKKGLYYQAHLTLAKSMG
                     DDWSTSGENAFDRRRDRSQGGTIPRWRAVAIALYELPFGRGKTFGSSMPAVLNHIVGN
                     WNMGGTYVWQTGLYFTPAYAGLDASNTNQFGGRPDRVRDGNLSKSERSLERWFDTGAF
                     VAPPAGAGRYGNSGAYILEGPGISVFHFGVNKDIVLHERAKLKLEMASTNFLNHPNFA
                     NPVNTIGTSTYGSILSTGGRASSSSGEGPRDFQFTARFIF"
     gene            complement(4938..>5848)
                     /locus_tag="VFQ79_18200"
     CDS             complement(4938..>5848)
                     /locus_tag="VFQ79_18200"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0141661.1"
                     /translation="RVVARHGKLYATHMRSYFGGILESIDEQLELARRSGCRLQISHL
                     QIVGRKNWDLQKKVIDKIEQAHQQGVDVAFDCYPYIAGSTVLTQILPQSALEGGIAAM
                     LARFADPAARARIASETLENLAWAWSDVYISAVASAKNASLVGKNLEEISKEWQREPV
                     QTVMDLLTEENGAVNMLCFNQSEENLRQTLAHPLSMIGSDGFYVKGRPHPRLAGTFPK
                     LLGYYTRQEKLFPLEEAIRKITSIPAERLRLNDRGVLAPGNVADIVVFHPQEIAGPAD
                     YENPEVPPTGIRYVFRNGNRTDNGRR"
BASE COUNT         1169 a         1700 c         1748 g         1231 t
ORIGIN      
        1 ccagacgagc gactggcggt cacgatacca ggcgctgcag ttgcgcgtgc aacggtccta
       61 cagcgccggg gcaaccatcc tggtggcgta taactacaac caggaacgta acgaagcgtt
      121 ctttaacgac ccgcagcatt acgtgaacca ggtgttctgg ctggggtcga acaatgcgcg
      181 gcaccggctg accctggcgg ggacttacga cttcccgatc ggtaaaggcc ggaagatcgg
      241 cggtggcatg catccggtgc tgaatgccgt tgccggtggg tggcaggtga gcggcattta
      301 cacctaccgt tccggcgaat atctccgttt tggcggcatg gacctgattg gcgatccagt
      361 gatcgacaac ccaggtcccg gaggatggtt taacacggat gcattccgca ttcagaccgc
      421 cttcacgcct cgaatgaacc cgtaccagtt ctccgacgtt accggcccca ttttctggaa
      481 tctggacggc accctgtcca agaacttccc gatcatggag cgatacacac tggagttccg
      541 cttcgaggcc tacaacctga ccaactcgct gatgtgggcg aaccccaaca tgaccgtggg
      601 taacgcgcag ttcggccgct ccggtgcgca ggccaaccag aaccgcgggc gcgaaatgca
      661 gtatacgctg cggttcattt tctaattgtt ctgaaggtgt gcgggggcgt taccgcgctc
      721 ccgcaccgaa tgtgtcacag gcggagacat caccggtctc gaagccacgg cggaaccaac
      781 cggctcgttg ctcggaggaa ccgtgtgtga aagtttcggg actcacatgt ccccggccca
      841 ttcgctgaag acggtcgtcg ccgatggcgg cagccgcgtt caatccctcc tccacgtcgc
      901 ccttctccag gatgtcccgt gccgccgtgc tatgtcccca tacaccagcc aggcagtcgg
      961 cctgtagttc cgtacggacc gacagctcgt tggcgcttgc cacgcccgca tcctgtgcgc
     1021 ggcgcacctg gcggtcgata cccaacacgt gctgcacgtg gtgtcctatt tcgtgcgcca
     1081 gcacgtaagc ctgcgcgaaa tctcccggtg cgccgaaccg ctgatgcagt tcggcgaaaa
     1141 atgagagatc gatgtatact ttctcatccg cgggacagta gaaagggccc gtctgcgctt
     1201 ctgcgaaccc gcagcccgat tgcaccgtgt cgcggaacaa cacgagcttg gcatcccggt
     1261 aatttcttcc cgccgctggc agaatttgac gccaggtctt ctgaagatcg tcgagaacga
     1321 aagagacaaa ttggaccatc ggctcttcgt ttgcatcctg tatggggtct gaccgggtat
     1381 atggaacaga ggtctcgctc gtgctgagaa gcgacagaaa gtcctgcccg aagatgaagc
     1441 tcagcactaa catcaacaga aggcctccga gaccgagccc ggcgccgcca cggccgagcc
     1501 gaagaccgcc accgccggcg ccgcgacgat cttcgagatt agggctacgt ccaccagggg
     1561 tccagcgcat tcaatcctcc gcgcgggccg tagcacttga actgccacgg ccctttttgc
     1621 gggactgctt aaaatataaa gcgtgcggtg aactggaagt cgcgtgggcc ttcaccggaa
     1681 ctggacgatg cgcgcccgcc ggtgctgagg atgctcccgt acgtgctggt gccgatggta
     1741 ttcaccgggt tcgcaaagtt cggatggttc aggaaattcg tggacgccat ctccaacttc
     1801 aatttcgctc tttcgtgaag aacgatatct ttgttgacgc cgaagtgaaa tacactgatg
     1861 ccgggacctt ccaggatgta ggcaccggaa ttgccgtaac gccccgcgcc ggctggtggc
     1921 gcgacaaaag caccggtatc gaaccagcgt tcgagactgc gctcactctt ggagaggttg
     1981 ccgtcgcgga cgcggtccgg ccgtccgccg aactggttcg tgttggacgc atccaggccg
     2041 gcataagccg gagtgaaata gagccctgtc tgccatacat acgtaccccc catgttccag
     2101 ttgcccacaa tatggttgag gaccgccggc atgctgctgc cgaaggtctt tcccctgccg
     2161 aatggcaatt catagagcgc tatagcgaca gcacgccagc gcgggatggt tccgccctga
     2221 gagcggtcgc gcctgcggtc gaaggcattc tctcccgaag tgctccagtc gtcgcccatg
     2281 ctcttggcca gcgtaagatg ggcctgatag tacaagccct tcttgaactg gcgctcggct
     2341 ttgatcacac cggcgtgata gctgtggctg ccgccatttt ccgtgtaact cgcggagaaa
     2401 agataaggaa atggcaccag cggcaacttt tccgcatagg gccgcgtgtc tgccggcggc
     2461 tggttcaggt tccggctcca gacgttcttc cggctcagcg tcgacatgta ggacacggaa
     2521 aaccccatat cgaaagcttc tttttccatg gtcacgttgt attgcgtgct gtatggtgtg
     2581 cggaagtcgg ggctcatccc acttgcgcca gtgccgccaa ggacatatgc cgtgcccggg
     2641 aacgggcgcg gaaacgagaa tgccggcaca ccgccgctga tcgcgttcgt gaacgtctcg
     2701 cgcaactcgt agggcgcacc gccgccgtcg gaaatatacg gctgcatggc cacgtagtag
     2761 acccctgccc cgccgcggat cacaaagttg ttccagaact ccgggcgcca cgcaaatcct
     2821 acacgtggcg cgaagttgtt tttatccagg ttcaatagct ttgcgcccag ccccaccgaa
     2881 ttcgccgtca caatcgggat gcttgccggg aagccgggat gcactctggc cctggccgcc
     2941 tcatccggca ctacgagcgc attataagcc ggcacgaagt tggcgagcag gttctgctcg
     3001 atcatacccg gatcgagcag cgaatagcgc aggccgtagt tcaacgaaaa gcgcggggtg
     3061 attttgaaat cgtcggtgaa gaacagttcc cagttcagcc ggtggggata gaccggaccg
     3121 accgaagtgc ggcgatcgct ggtatccatc aggcccagaa gaaaatcggc gtacggatct
     3181 ccgctgaagc ggccgttgaa gccgaatatt ccgaaaacgg cgctcggcga tgtcgcatac
     3241 tgctttccgt tgtatttgcc gacttccagt cctacgcgga aattgtgttt gccgcgggtg
     3301 atgtgaaacg tctcctgcac gtggaagttg ctccacatcc aaccgttcat gtcgccctga
     3361 tagataggct ggaacccggt gatattcatg cgcggcaatc cgggcacgtc ctgcaggtcc
     3421 gccagttgca acccggcggc ctgtacgatc ggaagtccgg gattcgggcc gccgtattcg
     3481 ctctggtctc tggcataacc cacgcggaat tcgttcagca tggagggtga aattacgtgc
     3541 gtgtcggaga gaacaacgtg atactggtcg cgccactggc tgtaattgcc aatgttcggg
     3601 ttggcgcctc cgtcccaggt ctcgaattca tagtgctggt acatgaagcg gccgaagaag
     3661 gaattggcgt tactgatctt gtggtcgccg cgcgcagtgt actgatcgct cgtattcggg
     3721 aacacggcga cgttgttata gcggtccgtg ccgctgttcg gcagcgggta aaacgtatcc
     3781 tggtagtatt tcgccaccgg gctgattcgt gattcaggga tgcggttgtt cgggaatgcg
     3841 ctgccggtcg aaggatcgat gatgggagtg ccgagtgagg cgaaatcgcc ggctcgaaag
     3901 gctgccgtcg ggacgacgaa gttcgccacc gtaggcgtct ggcttccgcg caacccctgc
     3961 caggagaaat gccagaacgt ccggtcgcgg ccgttataaa gcttccctaa atacagggga
     4021 ccgccgatct tgatgttcgg ccggaaaatg ggtggcccgt gtggacgtgc gggcgcgaag
     4081 aaatcgcgcg cgccgaagcc gatggcattg tagtgaagcc atgcttcccc gtgaagctgg
     4141 ttggttccgc tttcggaaac tacggcgacg ttggaactgg tgccgcccga agcgtttaca
     4201 ctgaccaggt ccacgcgata ctcgcggtag gaatccaggt aagtaagggc gggaccgaag
     4261 gaactgccgg tccaaccatc cttgatcgga attccatcga tcgagtaagt cgtattccgt
     4321 ccctggccgt tgcctgcgaa cgtgctgcca cttactccag ggctcagcac cagcatgcgc
     4381 cagatcgaac ggtaattgga gttaatcacc atgttggtga aaacgtagtt cgacttcgtg
     4441 tcggagatgg ttgcgcgctc tgtttcgatc agttgcgcgc cgccctccac ggtgacagat
     4501 tcggtgacct gcccgacctc gaggcgcaca tccacacgac ggtcagccgt cgccgtcaat
     4561 tcgatgtcct cgacaacgaa ccgtttgaag ccgggaggct ccgccgtaac ccgatagcgg
     4621 ccaggaatca agttcggcgc tacataattg ccgaccgaat tactttctac gtcctggctg
     4681 atgttggtat caatattcgt cacggtgact tttacaccgg gagttatcgc gcctgtggca
     4741 tcggtgatag tgccgcgtat agttccgtaa tacgtctgcg aaaccgcggt tccggaacag
     4801 atgactagca ggtacagtga aagaacactt ctggctgtca tagattcctt ccctgagaga
     4861 tttggtacaa cagtacttcc aggactatta ggaagtcaag gggcggatag ctgaaattgg
     4921 gctgatgatg ggcggtgcta tcgcctgccg ttatcggtcc ggtttccgtt ccggaatacg
     4981 tatcggattc ccgtgggagg aacctcgggg ttctcatagt ccgccgggcc ggctatttcc
     5041 tgcggatgga aaaccacgat gtcggcgacg ttgccgggcg cgagcacccc gcggtcgttc
     5101 agccgcagcc gctccgccgg gatgctggtg attttgcgga tggcctcttc gaggggaaac
     5161 aacttctcct gccgggtgta gtatccgaga agtttcggaa acgttccggc caggcgtgga
     5221 tgcggccgtc ccttcacata gaagccgtcg cttccgatca tggatagcgg atgcgcaagt
     5281 gtctggcgca gattctcctc gctctggttg aagcagagca tgtttaccgc accattctct
     5341 tccgtcaaca ggtccatcac cgtttgaacc ggttcgcgct gccactcttt cgaaatctct
     5401 tccaggtttt tgcccaccag cgaagcgttc ttggccgacg ccacggccga gatgtagaca
     5461 tcggaccatg cccaggcgag gttttcgagt gtttcggagg cgatgcgcgc ccgtgcggcc
     5521 ggatcggcaa agcgcgcgag catggccgcg atgccgcctt ccaacgccga ttgcggaaga
     5581 atctgagtga gaacggtact tcccgctatg tatgggtaac agtcgaaggc gacatcgacg
     5641 ccctgctggt gggcctgttc gattttgtcg atcacctttt tctgaagatc ccagtttttg
     5701 cggccgacga tttgaagatg ggagatttgc agccggcagc cgctgcgccg tgcgagttcc
     5761 agttgctcat caatcgactc caggatgccg ccgaagtagc tgcgcatatg ggtggcgtac
     5821 aatttgccat ggcgggcgac gacgcggc
//
LOCUS       DASTMK010000423         9214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_212690, whole genome shotgun sequence.
ACCESSION   DASTMK010000423 DASTMK010000000
VERSION     DASTMK010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212690"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFQ79_18205"
     CDS             complement(<1..1011)
                     /locus_tag="VFQ79_18205"
                     /inference="COORDINATES: protein motif:HMM:NF016220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEU0141662.1"
                     /translation="MTDYREKALEDAIEQSLVNTGGYRAVPESAFDRESALIASEALT
                     FVQGTQEKVWKVLAAYYGSRAEAEFIAELRKALDARGTLDVLRHGLEFFGQTFRLAYF
                     APASGLNPDIAKLYAANRLGATRQVHFDTRNEQSVDLMLSLNGIPVATAELKNHFTGQ
                     TVRDAISQYRGRDIRGIALFRFKTRALVHFAADPDEVYMTTRLEGEATRFLPFNRGRN
                     RGAGNPDNPGGHKSAYLWEQIWAHDSWLDLIGRFLHLERIEKPDPATGKTRVVENLIF
                     PRYHQIDAVRKLVADARESGAGKSYLIQHSAGSGKSNSIAWLAHRLASLHDAQDRKVF
                     DSV"
     gene            complement(1008..2249)
                     /locus_tag="VFQ79_18210"
     CDS             complement(1008..2249)
                     /locus_tag="VFQ79_18210"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU0141663.1"
                     /translation="MAELNPPTPRLSGEMPYVPMEAIGLGGGLCVDTTRNIDQESSGL
                     TYFQDGDVLIAKITPCFENGKCALATGLPDGHAVGTTELHVLRCGPRLRPKFLFYVVQ
                     SHTFRQAGEASMTGAAGQKRVTPEFIANYGVLLPDHVEQDAVVDFLNRATLEIDELVA
                     KKQRLIELLEEKRAALISRAVTQGLDAGVPMKDSGVEWLERVPAHWRLGRVKWLARTQ
                     SGGTPNSQDAGLYGGEIPWLRTLDLTNGPVSNAPILITEEALMQIAGDVLPTGSVLIA
                     MYGGEGTIGKFGILEIEAAVNQAICAILPCAAVLPTFLGTYIEFYRPFWMADAQGTRR
                     DPNISQDIVRRLMVPLPPVAEQQRIAEYVEREATKLQRIAAGCRATIGRLREYRSALI
                     TAAVTGQLDLKKHEKQMEALA"
     gene            complement(2288..4303)
                     /locus_tag="VFQ79_18215"
     CDS             complement(2288..4303)
                     /locus_tag="VFQ79_18215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEU0141664.1"
                     /translation="MTVYSDKLVSFIWDVADLLRGPYRPPEYRKVMLPMTVLRRLDCV
                     LAGTKVKVLDTAKAVKGQPATLIDRRLKQAAKQPFYNASKYDFEKLKGDPDGLAQNLL
                     NYIRGFSPDVRKVFDNFAFDQQIAKLDQTNRLFKLVSKFSEVDLHPDRVSNLEMGRTF
                     EELVRKFNEAANEEAGDHFTPREVIRLMVDLLFGPDDDALTRKNVVKTLFDPAAGTGG
                     MLSEASKYIRELNPDATLVLFGQDYNPEAYAICGSDMLMKGEDIDHIAFGDSLGDGRT
                     SDAFPDTKFDYMLANPPFGVKWEAQQERLRREHEDLGYNGRFGAGLPRINDGSFLFLQ
                     HMISKMWPYSHRERTGSRVAIVFNGSPLFTGDAGSGESNIRQWIVENDWLEGIVALPD
                     QLFYNTGIHTYIWVVTNRKSRKRKGKVQLVNATGFFEKMRKSAGNKRNLISEEQIGEI
                     ARLYEAFEEGEHSKIFDNEDFGYRKITVERPLRLRFEVTEEGLERLAEARAFAALATS
                     RKSGKEGRAEIGRGQALQEQIVAALRKLAGEVWLSRPVFEGVLDGLPVSMKAPVRKAV
                     LAAMTERDESAEVCLDQDGKPEPDPELRDTENVPLKEDVQEYFRREVLPHVPDAWVDE
                     SKTKVGYEIPFTRHFYKYTPPRPLEEIERDIRELEGEIVRMLAAVGR"
     gene            complement(4412..5623)
                     /locus_tag="VFQ79_18220"
     CDS             complement(4412..5623)
                     /locus_tag="VFQ79_18220"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU0141665.1"
                     /translation="MVPSGILSGIALLLIGGNAIALAQSPASNASPAQDRRVCAAQPA
                     ARLINWRLEPAKVLEEVDRSLTALEQIVHRAGQAGCDVLALPEDTMGVLHWEMGNTGR
                     MNQVLPEAVTRMLTRLGKVAASYRMYLVCSSDVAEQDGSYRNTAFLLGRDGREIGRYY
                     KVNLPLQESSRKRGDHFPVFETPDLGGVGMLICYDMMMPEATRSLALGGADIVFVPTL
                     GGAAFGERDMSRAAFRTRAVDNFIYLVVSHRGGGAMIISPQGKVLAEGKEPNDIAMAD
                     INPFGGREGADALNSQTDMRARLFRERNPAAYGILTDPNPPVLKKIPEVTTAEEAVRV
                     GAQALTIGEERFRDAEALLNSGKTADAIAAFEKLRTDFPRTWIDRVSRQRLEKIRASA
                     TSQAPQSSTPR"
     gene            5695..6948
                     /locus_tag="VFQ79_18225"
     CDS             5695..6948
                     /locus_tag="VFQ79_18225"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141666.1"
                     /translation="MDRRLFLSTGVSSLVSSQLGAASDRVRIAVIGMGGRGSELMRVF
                     SGVAGVEVATVVDPDGNRGEKAAGWLLQKTGKRATVETDMRRVFDDKTIDAVIIATTN
                     HWHALTAIWAMQAGKDVYVEKPVSHNFFEGQKLVEAARKYNRIAQGGTQRRSMGRFRK
                     AMQLLHEGAIGDIYLAKWFFPGSRNSIGFKPVEPPPSWLKWDLWLGPAPEQPYHGNLV
                     HYNWHWFWDFGNGEMGNNGIHLVDISRWGIGKELPVKVHCWGGRFGYKDQAETPNTQT
                     VTWQYADGSAIVGEIRGLYTTEPMYWDFFGSKGHLHIAANGEFQILLGRNKQTEPPVE
                     PLPEMNHYANFAEAVRTRNRDMLAAEIRETHLSTALCHLGNIAYRVGREVRFDPAAGR
                     FIDAPDADKLLTRQYRPPYVVPDRI"
     gene            6989..8200
                     /locus_tag="VFQ79_18230"
     CDS             6989..8200
                     /locus_tag="VFQ79_18230"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0141667.1"
                     /translation="MSTTRRTLLRGAGFGSLFTAWPFSKLSAARRGPVKVYEELGVRP
                     VINFQGTHTTIGASKMWPELHDAMAEASREYVSLEELQTKAGERIAKLMGAEAAMVTT
                     GAAGAITLGTCAVLTGEDTSKVRRLPEITGPKTEVIIQKVHRNGYDHAVRNAGVRIVD
                     VENREQFQNAIGPQTAMMYYLGGTSHDWEWETPIPLEECLTIAKRAGFPVMVDAANML
                     PPWENIRKLAATGVELICLSGGKHMRGPQCSGILAGRKDLIRAAWLNSSPHSDALGRP
                     MKAGREEIVGLWLAAEKYSRLDFAAIDRECAGQADYLIAQLSKIGGVKAERTPFDRTR
                     RVHRVHVQWDEAAAGLTTNEMIQRLKEGDPPIVVLRGKPQGIELTVFMNEPGDEKVAA
                     RRIKQIFEKRA"
     gene            complement(8204..>9214)
                     /locus_tag="VFQ79_18235"
     CDS             complement(8204..>9214)
                     /locus_tag="VFQ79_18235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0141668.1"
                     /translation="EVFDQIAIPAHTFGGWFDIFSQGTLRGYVGMSQRGKGETARRQS
                     RMVIGPWGHGSSQRTGAIDFGPHAHVDTHAIELRWFDYWLKGIDNGLDKEPPVTLFVM
                     GKGIWRQENEYPLARTEYRKMYFSSGGRANTRLGDGRLSWDEPPADSKPDRYRYDPDD
                     PVPSLGGNNCCGTPTPAGPQDQRPIETRQDVLVYTSDYLKEDLEVTGPVKVVLHASSD
                     APDTDFVAKLIDVFPDGRAINVAEGILRARYRESLSRPVPLQPGRIYELPIDLVGTSN
                     AFLKGHRIRVDITSSHFPQFDRNPNTGEPFGRHAGVRIANQTIHHTRAHASHIVLPVI
                     PK"
BASE COUNT         1783 a         2952 c         2626 g         1853 t
ORIGIN      
        1 caccgaatcg aacaccttgc ggtcctgagc gtcatgcagg ctcgcgagcc ggtgcgccag
       61 ccacgcgatt gaattgctct tgccgctgcc cgccgaatgc tggatcagat agctcttgcc
      121 ggcgcccgac tcccgtgcgt cggcgaccag tttccgcacc gcgtcgatct ggtgatagcg
      181 cgggaaaatc aggttctcga caacgcgtgt cttgccagtc gccggatcgg gcttctcgat
      241 tctttcgagg tgcagaaatc gtccgatcag gtcgagccac gaatcgtgcg cccagatctg
      301 ttcccacaga tacgcgctct tgtgtccgcc gggattgtcc ggatttcccg caccccggtt
      361 ccggccacgg ttgaacggga ggaatcgcgt ggcttcccct tccagccgcg tggtcatata
      421 gacctcatcg ggatcggccg cgaagtgaac cagtgcccgc gttttgaacc ggaacagcgc
      481 gatgccgcga atatcccggc ccctgtattg cgagatcgcg tcccggactg tctgtccggt
      541 aaaatgattc ttcaattccg ccgtggccac cgggatgccg ttcaggctga gcatcagatc
      601 cactgactgc tcgttccgtg tatcgaagtg gacctgccgc gttgccccaa gccggttcgc
      661 tgcatacagc ttcgcgatgt cgggattcag cccgctcgcc ggagcgaagt acgccagtcg
      721 gaacgtctgg ccgaaaaact cgagcccgtg ccggagcaca tcgagggtcc ctcgcgcgtc
      781 gagcgccttg cgcagttcgg caatgaactc cgcttccgcg cgtgagccgt agtaggccgc
      841 aagcaccttc cagactttct cctgcgtgcc ttgaacaaac gtgagcgcct ccgaagcgat
      901 cagcgccgac tcgcgatcaa acgcggattc cggaactgct cggtacccgc ccgtgttcac
      961 gagcgattgc tcgatcgcgt cttccagcgc cttctcgcga tagtcggtca cgccagcgcc
     1021 tccatctgct tctcgtgctt cttaagatcc agttgccccg tgacggcagc ggtgatcaga
     1081 gcggagcggt actcacgaag gcggccaatc gttgccctgc aacccgccgc gattcgctgc
     1141 agcttcgttg cttcccgctc gacgtactca gcgattcgtt gttgctctgc gacaggcggt
     1201 agagggacca tcaatcgtcg tacgatgtct tgactgatgt ttgggtccct tcgcgttccc
     1261 tgagcgtcgg ccatccagaa cggtcggtaa aactcgatat acgttccgag gaacgtcggc
     1321 agcacggctg cgcacgggag aatcgcacaa atcgcttggt taacggcggc ttcgatttca
     1381 agaatgccga acttgccgat cgtgccttca ccaccgtaca tcgcgatgag caccgacccg
     1441 gtgggaagca cgtcaccagc tatctgcatc aacgcctcct cagtgatcag gattggcgcg
     1501 tttgacacag gaccgttcgt caaatccaga gttctgagcc atgggatctc gccgccgtat
     1561 agacccgcgt cctgcgaatt tggcgtccca ccgctctgcg tccgggccaa ccacttcact
     1621 cgacccaacc tccagtgtgc cggcacgcgt tcaagccact ccaccccgga gtccttcatc
     1681 ggcacacccg catccaaccc ctgagtcacc gcccggctga tcagcgccgc ccgcttctcc
     1741 tccagcagtt caatcagccg ctgctttttt gcgaccaact catcgatctc aagggtcgcg
     1801 cgattgagaa aatcaacaac cgcatcctgc tccacatgat ctggcagtag cacgccgtag
     1861 ttggcaatga actcaggcgt tacgcgcttt tgcccagcag caccggtcat gctcgcctca
     1921 ccagcttgcc gaaaggtgtg cgactgcaca acgtagaaca ggaatttcgg ccgcagtcgt
     1981 ggcccgcagc gcagaacgtg taactcggtt gtgcccaccg cgtgcccgtc aggaagtccc
     2041 gtagccagag cacacttccc attttcgaag catggggtga tcttggctat caatacatcc
     2101 ccatcctgga agtacgtcag gccggacgat tcctgatcta tattgcgcgt cgtgtccacg
     2161 cacaacccac cacctaaccc tatcgcttcc atcggcacgt agggcatctc gccggaaagt
     2221 cgcggcgtcg gcgggttcaa ctcggccacg aatcgaagtc gaatggcaga ccaacggtta
     2281 cggagcatca ccgccccacc gccgccagca tccgtacaat ctccccttcc aattcccgaa
     2341 tgtcccgctc gatctcctcc agcgggcgag gcggcgtgta cttgtagaaa tgccgcgtaa
     2401 acggaatctc gtagccgacc ttcgtcttgc tctcatccac ccacgcatcc ggcacatgcg
     2461 gcaatacctc gcgccggaag tattcctgca cgtcctcctt cagcggcacg ttctccgtat
     2521 cccgcaactc cggatcgggc tcgggcttgc cgtcctggtc aaggcaaacc tccgccgatt
     2581 cgtcccgctc cgtcatcgcc gccaaaaccg ccttacgcac tggagccttc atcgaaaccg
     2641 gcagtccgtc cagcacccct tcaaacacgg ggcgcgacag ccatacctcg cccgccaact
     2701 tacggagcgc ggcaacgatc tgctcctgca acgcctgtcc ccggccaatc tccgcccgcc
     2761 cttccttccc tgacttgcgc gacgtcgcca gcgccgcaaa tgcccgtgcc tccgcgagcc
     2821 gctccagccc ttcctccgtt acttcgaacc gcagccgcag cgggcgttcc acggtgatct
     2881 tccggtaacc gaaatcctcg ttgtcgaaga tcttcgagtg ctcgccctct tcaaacgctt
     2941 cgtaaaggcg cgcaatctcg ccgatctgct cctccgaaat caggttgcgc ttgttgcccg
     3001 ccgacttgcg catcttctcg aagaatcccg tcgcgttcac aagctgcacc ttgcccttgc
     3061 gcttccggga cttccggttc gtcaccaccc agatatacgt gtgaatcccc gtgttgtaga
     3121 acaactggtc cggcagcgcg acaatcccct ccagccagtc gttttcgacg atccactgcc
     3181 ggatgttgct ctcgcccgat ccggcatcgc ccgtgaacag cggcgacccg ttgaacacga
     3241 tggcgaccct cgaccccgtc cgctcccgat gtgagtacgg ccacatcttc gaaatcatgt
     3301 gctgcaggaa cagaaacgac ccgtcgttga ttcgcggcag gcccgccccg aaccgcccgt
     3361 tatacccaag gtcttcatgc tcgcgccgca accgctcctg ctgcgcttcc cacttcaccc
     3421 cgaacggcgg gttcgccagc atgtaatcga acttcgtgtc cggaaacgcg tccgacgtcc
     3481 tcccgtcccc aagcgagtcc ccgaacgcga tatggtcgat gtcctcgccc ttcatgagca
     3541 tgtccgaacc gcagatcgca tatgcctccg ggttgtaatc ctgcccgaac aacaccagcg
     3601 tcgcgtccgg gttcagttcc cggatgtact tcgacgcttc cgacaacatc ccgcccgttc
     3661 ccgctgccgg atcgaacagc gttttcacta cgttctttcg cgtcagggcg tcgtcatccg
     3721 gaccgaatag cagatccacc atcaaccgga tcacttctct cggcgtgaag tggtctcccg
     3781 cttcttcatt cgcggcttcg ttgaacttgc ggacgagttc ttcgaacgtc cggcccatct
     3841 ccaggttcga gacccggtcc ggatgcaggt ccacctcgga gaacttgctc acaagtttga
     3901 aaagccggtt cgtctgatcg agcttcgcaa tctgctgatc gaatgcgaag ttatcgaaga
     3961 ccttgcgtac gtccggagaa aacccgcgaa tgtagttgag caggttttgc gccagtccgt
     4021 ccgggtcgcc tttcagcttc tcgaaatcgt atttgctggc gttatagaaa ggctgcttcg
     4081 ccgcttgctt cagcctccga tcaatgagag tcgcaggctg ccctttgacc gccttcgcgg
     4141 tatcgaggac cttcaccttc gtacccgcga ggacgcaatc cagccgccgc aggaccgtca
     4201 tcggaagcat caccttccgg tattccggag gccggtacgg cccccgcagt agatccgcca
     4261 catcccaaat aaagctgaca agcttgtcgc tgtaaacagt cacgccggtt accctgattg
     4321 agaaccttta gcgtatacca ggctcggaca cgggcgtgcg ccgattatta cataacgtat
     4381 acgctgactg acggggatct ctatagtgtt ttcagcgcgg cgtcgagctt tgcggagcct
     4441 ggctcgtggc gctggcccgg atcttctcca gacgctgccg cgatacgcgg tcgatccacg
     4501 tgcgaggaaa gtcggtgcgg agtttttcga aagccgcgat ggcgtccgct gtcttgccgc
     4561 tattgagcag cgcctccgcg tcgcggaatc gctcttcgcc gatggtgagt gcttgcgctc
     4621 cgacgcgcac cgcctcctcc gccgtggtaa cctcgggaat ctttttcagc acgggagggt
     4681 tggggtctgt aaggattcca taagcggcgg gattccgttc ccggaagagc cgtgcgcgca
     4741 tgtcggtctg cgaattcaga gcgtcggccc cttcgcgtcc gccgaatggg ttgatatcgg
     4801 ccattgctat atcgttcggc tctttccctt ccgccagcac ttttccctgc ggtgagatga
     4861 tcatggctcc gccgccgcgg tgggatacga caagatagat gaaattatcc actgcgcgtg
     4921 tgcgaaaagc ggcacggctc atatcccgtt cgccgaatgc cgcgccgccc aatgtcggga
     4981 caaacacgat gtccgcgccg ccgagggcga ggctccgcgt ggcttcgggc atcatcatgt
     5041 cgtagcagat caacataccc acaccaccca gatcgggagt ttcaaacacc gggaaatggt
     5101 caccgcgctt ccggctggac tcttggagag gaagattgac cttgtaatac cggccgattt
     5161 cccggccgtc gcggcccaaa agaaaagcgg tgttgcggta gctgccatcc tgctctgcca
     5221 catcactcga acacaccagg tacatccggt agctggctgc tacttttccc aaccgcgtga
     5281 gcatccgcgt gaccgcctcg ggaaggacct ggttcatccg cccggtgttg cccatctccc
     5341 aatgcagcac acccatcgtg tcttcgggca gtgccagaac gtcgcacccc gcttgtccgg
     5401 ccctgtgtac gatctgctcc agggcggtga gcgaacggtc gacttcctcc aataccttcg
     5461 ctggctccag gcgccagttg atcagccgcg cggcgggttg cgccgcgcaa acacgcctat
     5521 cctgcgcagg cgacgcattg gaagcagggc tctgcgccag ggcgattgcg ttgccgccta
     5581 tcagtagcag ggcgattcca ctcaatattc cggatgggac cacaacaaac ctctcccatt
     5641 gattttgcct cagcggccga tgaaatggga aagggcttcg cgcataatct aataatggat
     5701 cgcagactct ttctttccac gggggtatcg tctctcgtct cctcgcaact cggcgccgcc
     5761 agcgaccggg tacggatcgc cgtcatcggc atgggagggc gcggtagcga actcatgcgc
     5821 gtgttctccg gtgttgccgg cgttgaggtt gccaccgtgg tggacccgga cggcaatcgc
     5881 ggtgagaagg ccgccggatg gctccttcag aagaccggta agcgcgcgac agtggagacc
     5941 gatatgcgcc gcgtgttcga tgacaagacc atcgacgctg tcatcattgc cacgacgaat
     6001 cactggcatg cgctgaccgc tatctgggcg atgcaggcgg gaaaagacgt ttacgtggaa
     6061 aagcccgtgt cgcacaattt cttcgaaggc caaaaactgg tggaggcggc ccgcaaatac
     6121 aaccggatcg cgcagggcgg tactcaacgg cgctcgatgg gccgctttcg caaagccatg
     6181 cagttgctgc atgaaggggc gatcggcgac atttacctgg cgaaatggtt tttccccggc
     6241 agccggaatt ctatcggctt caagccggtt gagccacccc catcctggct caaatgggat
     6301 ctgtggctgg gtcccgcgcc cgagcagccc tatcacggca acctggttca ctacaactgg
     6361 cactggttct gggatttcgg aaatggcgaa atgggcaaca acggcatcca cctggtcgat
     6421 atttcgcgat ggggcatcgg caaagaacta ccggtgaaag tgcattgctg gggcggacgc
     6481 ttcggctaca aggaccaggc cgagactccc aatacgcaaa cggtgacgtg gcagtatgcg
     6541 gacggcagcg ccatcgttgg cgagattcgc ggcctctaca cgacggaacc gatgtattgg
     6601 gacttttttg gatcgaaggg ccacctgcat atcgccgcca atggtgaatt tcagatcctg
     6661 ctcgggagga ataagcagac ggaacccccg gtggagccat tgcccgagat gaatcactac
     6721 gcgaacttcg ccgaggccgt acgaacccgg aatcgtgata tgctggccgc cgagattcgc
     6781 gaaacgcatc tctcgacagc gctgtgccat ctggggaata tcgcttatcg tgtagggcgc
     6841 gaggtgcgct tcgatccggc ggcggggcgg tttatcgatg ctcccgatgc cgacaagctt
     6901 cttacgcggc aataccggcc gccttatgta gtgccggacc ggatctagaa tcgagcggta
     6961 aacgatactc gaatcgcgga ggtgttccat gagtacgacg cggcggactt tattgcgggg
     7021 cgccggattc ggctcgcttt tcaccgcctg gccgttttcg aaactgagcg ccgccaggcg
     7081 aggcccggtg aaagtctacg aagagctcgg cgtccggccg gtcatcaatt ttcaaggtac
     7141 acacacaacc atcggcgcct ccaagatgtg gccggagttg cacgatgcca tggccgaggc
     7201 ctcgcgcgag tacgtctcac tcgaagaact gcaaacgaag gcgggtgagc gtattgcgaa
     7261 actaatgggg gcggaggcgg cgatggtaac caccggcgcc gcgggcgcga tcacgctcgg
     7321 cacgtgtgcc gtactgaccg gcgaggatac ctcgaaggtc cggcgtctgc ccgaaatcac
     7381 cggcccgaaa actgaggtga tcattcagaa agtacatcgc aacgggtacg accacgccgt
     7441 ccggaacgct ggcgtgcgca tcgtggacgt cgaaaaccgg gagcagttcc agaacgcaat
     7501 cggaccgcag accgcaatga tgtactacct cggcggcacc agccacgatt gggagtggga
     7561 gacccccata ccactcgagg agtgtctgac catcgcaaaa cgcgccggat ttccggtgat
     7621 ggtggatgcg gccaacatgc tgccgccgtg ggagaacatc cggaagctgg ccgccaccgg
     7681 cgtcgagctc atctgcctca gcggtgggaa acacatgaga ggcccgcagt gctccggcat
     7741 tctggcggga cgcaaagatc tgatccgcgc cgcatggctg aattccagcc cgcattccga
     7801 tgcgctcgga cggcccatga aagccggccg cgaagagatc gtcggattgt ggctggcggc
     7861 cgagaagtat tccaggctcg acttcgcggc tatcgaccgc gagtgtgccg gacaggcgga
     7921 ctacctgatt gcccaattat cgaagattgg cggtgttaag gcggagcgca caccgttcga
     7981 ccgcacacgc cgcgtacatc gtgtacatgt gcagtgggac gaagccgccg cgggcctcac
     8041 gacgaatgaa atgatccagc ggttgaagga aggcgatccg ccaattgtgg tgctccgcgg
     8101 taaaccacag ggcatcgaac tgacggtctt tatgaatgag cccggcgacg aaaaggtcgc
     8161 cgccaggcgc atcaaacaaa tctttgagaa aagggcctga cccttacttc ggaatcaccg
     8221 gcagcacgat gtgcgacgcg tgcgcgcgcg tgtgatgaat ggtctggttg gcgatccgca
     8281 cacctgcatg acggccgaac ggctcacccg tgttgggatt gcgatcgaat tgcggaaaat
     8341 gactgctggt gatgtctacc cggattcggt gccctttcag aaacgcgttg ctggttccca
     8401 ccaggtcgat cggcagctcg taaatcctgc ccggctggag cggcaccgga cggctgaggc
     8461 tctcacgata acgcgcgcgc agaatgcctt cggccacgtt gatggcgcgt ccgtccggaa
     8521 acacgtcgat cagcttggcg acaaaatcgg tatccggagc atcggaggac gcatggagaa
     8581 ctaccttgac ggggccggtc acctccagat cctccttaag atagtctgac gtgtacacca
     8641 ggacatcctg ccgcgtttcg atcggacgct ggtcttgcgg accggcgggc gtaggcgtgc
     8701 cgcagcagtt gttgccgccg agactgggca ccggatcgtc aggatcataa cggtagcggt
     8761 cgggtttgga atccgcgggc ggttcgtccc agctcagcct tccatcgccg agccgcgtgt
     8821 tcgcgcggcc gccgctcgaa aaatacatct tccggtactc ggtgcgtgcc agtggatatt
     8881 cattctcctg ccgccagatg cctttgccca tcacgaacag ggtcacggga ggttctttgt
     8941 ccaggccgtt atcgatgcct ttcagccagt agtcgaacca gcgcaattct atggcatgcg
     9001 tgtccacatg cgcatggggg ccgaagtcga tggcgcccgt tcgttgcgag gagccgtgtc
     9061 cccaggggcc gatcaccatc cggctctgcc gccgtgcggt ttctcctttt ccccgctggc
     9121 tcattcccac gtagccgcgc aaagtgccct ggctaaagat gtcgaaccat ccgccgaagg
     9181 tatgcgcggg aatggcgatt tgatcgaaca cctc
//
LOCUS       DASTMK010000424        14001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_23212, whole genome shotgun sequence.
ACCESSION   DASTMK010000424 DASTMK010000000
VERSION     DASTMK010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23212"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1235
                     /locus_tag="VFQ79_18240"
     CDS             219..1235
                     /locus_tag="VFQ79_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU0141669.1"
                     /translation="MDLILFPFSEYWWFYGAFSAFVFLLLALDLGVFHRKAHDVSFRE
                     ATIWSVVWVSLALLFNFGLYQYAASHFPQAVARQVGFEFLTGYIVEKALSIDNIFVFV
                     LVFGYFGIPSKYQHRVLFYGILGAFVFRAIFIAMGSVLMQYHWVVVFFGAVLILTGVK
                     MFFAPDKQIHPEKNPIIRLFRKAVPVTPELHGQKFFVRLNQVVHATPLFVALLFLELS
                     DIVFAIDSVPAIFALTDEPMIVFTSNIFAILGLRAMYFMLASAVNKFHMLQYGLAFVL
                     VFVGLKMVWLNNWFGGKFPITVSLGIIAGAIGVSVVASLLVPPREKAPEQAGGSADQK
                     VLKV"
     gene            complement(1225..3993)
                     /locus_tag="VFQ79_18245"
     CDS             complement(1225..3993)
                     /locus_tag="VFQ79_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141670.1"
                     /translation="MKFVTLLLAAFCAMGADRLTLPVTADATVTFADLASKPEPPRGA
                     EPELVIQGRVSFALLQFDTSALKGLQVTKAMLRVHAAPSPVPLHTVGISTVSGNGPWK
                     ESEAIFYRPSPAGWWSYKGSDLVDVTFSQGGSLYVYPRVRDAGNGWFEADVSPDIVTA
                     LATGDQFGILLTDEKGQTRTRHVLSSKEGKHAPVLVVEADKPNAKVPAAPRGRHAGGT
                     LVFEGARARLDVRYAQSPIKDFAAAKAYPRWLIDPLAPKPHPLATSNSLSGTVTVKLD
                     DLAPGTYYFASRTLDERGTASPVTEVGPVRIDAPLSRKLPAADPVTGSDGPRSRVWTA
                     PDTVKINPVSGALLERDVVWNDGVKLTGARNEFVAFQLAVEGVQNGVTIEVSKPLFAG
                     NKLPAVLQKTGAIQLYREWFVRDDKNPNLFHPDPLVPLTRPFAIPSTDNPVPGQTVQP
                     VFVDIYIPHDAKPGVHTGELRVRSEAGLDQRVPVEVTVLPLMLPDKVSFVVDLNCYSG
                     VEGPTRGTPEYRAVEHAFHRVAHLHRANLDVLGYSHNGSTVPDHAPPLTGEGAETRVA
                     DWTDWDAHWGPLVSGELFQDMPRAGVPVDAMYLPFFENWPGDLRKHYRADNPAVAKTT
                     GEYEALITRHALTAGPVEEEFSKEYQDRFSAVALDFARHIKSRGWNTHTKFVVYFNNK
                     YYWKRPEQGGRGIGWWLLDEPNHRDDVRAISFLGWLAMRRLGEAPGSNILFRTDISRV
                     DWMRDLLTGQIDLNTISRRFHDKPRLLQDRNRFGKELWNYASTNHPRNTNVMLRAWAW
                     RVWLNGGDGLLPWLATRTQNAWKRTEPLTVFYSGAKFGQTEPFASLRLKAYRRGQQDM
                     EYLNLLAKSKGWSREILTRSVREALDLAAEVEEKYEEDAGTMRFQNIQNRDLETLRRQ
                     VATALVKP"
     gene            4049..4867
                     /locus_tag="VFQ79_18250"
     CDS             4049..4867
                     /locus_tag="VFQ79_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEU0141671.1"
                     /translation="MGPTPASEQPKLPIEGVRNLIAIGSGKGGVGKTTVAVNLALALR
                     SLGSKVGLLDADVYGPNVPLMMGLQETPHAIGNRIQPLEKYGIKLMSMGFLNPGDKPL
                     IWRGPMLHSVMQQFLRGVDWGELDYLLIDLPPGTGDVQLSLIQTAPLTGSIVVTTPSE
                     VSLEDARKAVNMFQQVRVEVIGLIENMSYLKCPHCSERIDVFSTGGGQKTAQAMGVNF
                     LGALPLDPQVRIGGDTGNPVALKDSSAFHEIGAQVLERVQAVGAPRGPSIEIED"
     gene            5010..8132
                     /locus_tag="VFQ79_18255"
     CDS             5010..8132
                     /locus_tag="VFQ79_18255"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEU0141672.1"
                     /translation="MRLAIMGINIGLDIGAISIKLAALGKPEDRPIVDRLGQPGSSFR
                     RIEDVCGNPAAISEYRRISGSPIQSAYDLLLDLYEFVPERLIEGIRVTGSGSRTIAKI
                     LGIYFENEFKAIARMFAAVHPEVRSIFELGGESSKYIRLEPGDDSGGCGILDYDRSGE
                     CAAGTGSFLDQQALRMGYSVEEVGDVVSCAGCGARIAGRCSVFAKSDMIHAQQKGYSP
                     AEILRGLCDAVARNFKSSIVKSRAIAPPVALIGAVSQNAGIAGALREVFRLGESDLMV
                     PQLYAWCGALGAAILEAEEPRKRSFRDIHKLNQHESVICRLDSKPLSMEKVVLLGDRV
                     DKYQPPEGDGPIAAYLGIDIGSVSTNLVAIDESANVIHDIYLRTAGRPIEAVQKGLTE
                     LKELWGHRLVIEGVGTTGSGRELIAEFVGADVVNDEITAHKTGAIHISDTLGGEPVDT
                     IFEIGGQDSKYISIENGVVVDFAMNEACAAGTGSFLEEQAEKLGISIKGEFARLALSS
                     QAPTRLGERCTVFMERDVTGWLHNGETVPNLVAGLAYSIVLNYVNRVVRGRKIGDVIY
                     FQGGTAYNDAVAAAFAKLLGKKITVPPYNGVMGAVGMALIARQWHRATRGFTRFRGYD
                     LNNLEMSSRDFVCKACSNECDIKEFKIEGQKSYWGDKCSDRYRKVSSTGRKPVIEDLF
                     AFREKLLEEITGAPLAPPKQLPRSDRICVGIPRTMPTYDQYPFWQHYFTAIGVDVVLS
                     RQTDPKIAADGVEMALAQPCYPVQIAHGHVQSLLDLGVDYVLTPNILNAEAHEDTVCT
                     SHMCPWNQTLPFVLQSAPRLEPHRHKFLTPTLHFQLGRQYVARTLAQMAQKLGVKRRV
                     SDAAVDAAYTAQRQFQERILEAGAAAMAALDESGEPGILLIGRGYNIYDRNINCDIPR
                     KLRHHYGANVIPLDFLVTGRERVQDLHPNMYWSSGQKILAASRLAAAHPNLHIIYITN
                     FKCGPDSYIKYFARQTAGVPLLVLQFDGHGNDAGYMTRCEAYLDSKGILRCYSSQPSS
                     EKAIRAAATH"
     gene            8075..9379
                     /locus_tag="VFQ79_18260"
     CDS             8075..9379
                     /locus_tag="VFQ79_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141673.1"
                     /translation="MLQQPTLERKSDPRGSHPLTGKTVYIPPMAYGSARAFAAGFRAL
                     GVDARLTPPSDERTKELGGRYTSGDECYPAKVTVGDFMRVLEQPSTDQVKTVFFMPTA
                     DGPCRFGQYATYLRRILDLNGFPSAQVLSPTSANGYEGLGNLARPFFRTGWRTLVAGD
                     ILQKLLLIYRPYETRRGETDEVYHVCLDDVCGTVERTTVEPSVQLKAIREALLRCRGR
                     FHAISARRDRSVPLIGVVGEIFCRLNTFSNDDLIRRLEEYGGEAWLSDIGEWIWYTNA
                     EQFRRLKLLGKQISTDAAKAWVRSKIQRHDEHVLLEPFRDDFRGYEEPPVEEILEYAR
                     PYLPCDGALGEMVLNVGKAIYLARKGADGILDISPFTCMNGIVCEAIYPRLSRDLGGI
                     PIRTFYFDGTQADLDRDLGVYLELARSYSRKKSTARVYPDRP"
     gene            complement(9408..9809)
                     /locus_tag="VFQ79_18265"
     CDS             complement(9408..9809)
                     /locus_tag="VFQ79_18265"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0141674.1"
                     /translation="MPSVVLDTHAAVWYLAAHPSLSISARQAIDGAQQPPYQVFVPTI
                     CLVELTYLIEKGKLHPEARARLISAIREGNSTFVEQPLTLEVAEAVSTVSRESVPDLP
                     DRIIAATAVALGVPLISRDSKIRAAQLTTIW"
     gene            complement(9809..10033)
                     /locus_tag="VFQ79_18270"
     CDS             complement(9809..10033)
                     /locus_tag="VFQ79_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141675.1"
                     /translation="MTVEESIVQAVRALPPNKKEAVLRYAESLQEPNGVEKPVRNLYG
                     LWKDLGPGPSAAEIDEVRHEAWKNFLRDDV"
     gene            10095..10277
                     /locus_tag="VFQ79_18275"
     CDS             10095..10277
                     /locus_tag="VFQ79_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141676.1"
                     /translation="MTSSLGRISFYADGRFVAEPKASREKPGQDILSKLESYSLDFRK
                     MRFSAEASNRQTPSGR"
     gene            10376..10594
                     /gene="infA"
                     /locus_tag="VFQ79_18280"
     CDS             10376..10594
                     /gene="infA"
                     /locus_tag="VFQ79_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU0141677.1"
                     /translation="MSKEDSIEVTGTVVEKFPSGLFSVQLDQERTVLAHLAGKLRRNR
                     IRVLAGDRVTMELSPYDLTKGRITYRHK"
     gene            10617..11732
                     /gene="wecB"
                     /locus_tag="VFQ79_18285"
     CDS             10617..11732
                     /gene="wecB"
                     /locus_tag="VFQ79_18285"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003772284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU0141678.1"
                     /translation="MPKILIVFGTRPEAIKLAPVVKELRSRSSHFDVQVCITAQHREL
                     LDQVLEVFSIEPEFDLDLMLAGQTLFQSTSRVVAGLESVLHQARPDMVLVQGDTTTTM
                     CGALAAFYARVPVGHVEAGLRTGTPGIPFPEEMNRVLTSRLASLHFAATEWAAANLRK
                     EGVAPERIVVTGNTGIDAVLHVHAGIESGRLVPCRNIPVDPGRKLILVTAHRRESFGE
                     GFEQICAALIELSRRPDVTIVYPVHPNPNVQEPVNRILEGAERILLVEPLDYVSFVGL
                     MRRAYLLITDSGGVQEEAPSLGKPVLVMRASTERPEAVEAGTARLVGTDARAIVREAS
                     RLLDDPEEYASMARVHNPYGDGRASRRIADAILSFFK"
     gene            11839..12894
                     /locus_tag="VFQ79_18290"
     CDS             11839..12894
                     /locus_tag="VFQ79_18290"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0141679.1"
                     /translation="MRNFRGGSKSKKETPGTVSGLKAGLHKRIVGQPAALEAIVPHIQ
                     IHQAGLSPEGRPAGVFLLLGPTGTGKTKTVEALAEVLHGSERNLLRIDCAEFQLDHEV
                     AKLIGAPPGYLGHRETHPLLTQAKLNSVVSERSPLSIVLFDEIEKAAPSLTRLLLGVL
                     DKATLRLGDNTAVNFERSLIFLTSNLGARDMARELSPGFGFDAMAGSRPALSGKLENV
                     AMRAVRKHFSPEFVNRLDTVVTYQPLDEKSLSEILDLQLAGLQQHIHSRRGCETFYLH
                     VAASGRKFLLNAGTSPEYGARELKRAIQRHLITPLAELIELGIEPGATVIADAPKNST
                     RLHFRMPAIRLAALDAA"
     gene            12965..>14001
                     /locus_tag="VFQ79_18295"
     CDS             12965..>14001
                     /locus_tag="VFQ79_18295"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0141680.1"
                     /translation="MGASEERQSGLRREIGLRDLVLFNIAAVIGIRWLAAAAHAGPGS
                     ISLWLLAALLFFVPSALAVSMLSRRFPEEGGLYIWTKRSFGDWHGFVAGWCFWLSNLF
                     YFPNLALAGVAMGVYTLGAQHTHLASNRGFVLGASLVLLWAALLSHLFGLRIGKWTQN
                     IGGLSTYAAGGLIIAAGAIVWALYGAATPIEMAPQWNWDKVNFWSQIAFAFGGLELGA
                     VMGGEIRNPKRNVPRAAWISGSAICVFYIAGTLAILALLAPENVSVLTGLAQAGQVAA
                     ERTGLAWFSPLLAGLITLGVVGQLGAWVGGSARVPFAIGLDRYLPPALGKLHPRWGTP
                     YISLLAQGVVCT"
BASE COUNT         2740 a         4097 c         4291 g         2873 t
ORIGIN      
        1 gcctcgcgct gcctggaatt ttgaaacctc cgggcggcgt actgcatcag accaactagc
       61 acgaggggag tagatcactc cttagagttt ggcggctcgt caagacggtt gtctagagac
      121 gatccgggcc gccagcgggc gaaagctcag atcgagacct cttgcggttc gagccgcaag
      181 aggtcttttt gcgttagggc ctcgcgcgca taaacctaat ggaccttatc cttttccctt
      241 tttccgaata ctggtggttt tacggggcct tctccgcttt cgttttcctt ttgctggcgc
      301 ttgacctggg ggtatttcac cggaaggcgc acgacgtgtc ctttcgcgaa gccaccatct
      361 ggagcgtcgt ttgggtcagc ctggcattgc tgttcaattt cggcctgtac cagtatgccg
      421 ccagtcattt cccccaagcg gtcgcgcggc aggtggggtt cgagtttcta acgggttaca
      481 ttgtcgaaaa agcgctttcg atagacaaca tcttcgtctt cgtactcgta ttcggctact
      541 tcggaattcc gtcgaaatac cagcaccgtg tcctgttcta cggcattctg ggggcattcg
      601 tatttcgcgc gatctttatc gccatgggat cggtgctgat gcaataccac tgggtggtgg
      661 ttttcttcgg cgcggtcctc atcctgaccg gtgtaaaaat gttcttcgcg ccggacaagc
      721 agattcaccc cgagaagaac ccgatcattc gcctgttccg taaagcggta cccgtcacac
      781 ccgagcttca cggccagaaa ttcttcgtgc gcctgaacca ggtggtgcac gccaccccat
      841 tgttcgtcgc tctgctgttt ctcgaactca gcgatattgt gttcgccatt gactcggttc
      901 cggctatttt tgcgctcacg gatgagccga tgatcgtctt cacgtccaat attttcgcca
      961 ttctcgggct tcgcgcgatg tatttcatgc tggcgagcgc ggtgaacaaa tttcacatgc
     1021 tccaatatgg gctggcgttc gttctcgtgt tcgtcggcct gaagatggtc tggctcaata
     1081 actggttcgg cggaaagttc ccgatcacgg tttcgctggg cattattgcg ggcgccatcg
     1141 gtgtctcggt tgtcgcgtcg ctgctggtgc cgccacggga gaaggcgccg gagcaggcgg
     1201 gtggaagcgc cgatcagaag gttctcaagg tttaacgagc gctgtagcta cttggcggcg
     1261 cagcgtctcc agatcgcgat tttgaatgtt ctggaagcgc atggtgccgg cgtcttcttc
     1321 gtatttctct tccacctccg ccgccaggtc cagcgcctcg cgcaccgagc gcgtcaaaat
     1381 ctcccgcgac catcctttcg atttcgccag tagattcagg tactccatat cctgctgacc
     1441 ccggcggtag gctttcagcc gcaaggaggc gaacggctcc gtctgcccga atttcgcgcc
     1501 gctgtagaac accgtgagcg gctcggtcct tttccacgcg ttctgtgtgc gtgtggcaag
     1561 ccatggcagc aggccgtcgc cgccattcag ccacacgcgc caggcccatg cccgcagcat
     1621 cacgttggtg ttgcgcggat gattcgtgct ggcgtaattc cagagttcct ttccaaaccg
     1681 gttgcggtcc tgaagaaggc gcggcttgtc gtgaaaccgg cggctgatgg tgttcagatc
     1741 gatctgtccc gtgagcaggt cgcgcatcca atccacacgg ctgatgtccg tgcgaaagag
     1801 gatgttcgag cccggcgctt ccccgagccg gcgcatcgcg agccagccga gaaagctgat
     1861 agcccgcaca tcgtcccgat ggttcggttc atcgagcagc caccagccga tgccgcgccc
     1921 gccctgctcc ggccgcttcc agtagtactt gttgttgaag tagacgacaa atttcgtgtg
     1981 tgtattccag ccgcgcgatt tgatgtggcg tgcgaaatcg agcgcgaccg ccgaaaagcg
     2041 gtcctggtat tccttgctga attcttcttc gaccggcccc gccgtcaggg catgcctggt
     2101 gataagtgct tcgtattccc cggtggtctt cgctaccgcc ggattatccg cccggtagtg
     2161 cttccgcaga tcgccgggcc agttctcgaa aaacggcagg tacatggcgt ccacgggtac
     2221 gccggcacgc ggcatgtcct ggaacagttc tccgctgacc agcgggcccc agtgcgcatc
     2281 ccaatccgtc cagtcggcca cgcgcgtttc cgcgccttca ccggtaagag gtggggcgtg
     2341 gtccggcacg gtgcttccgt tgtgcgagta gccgagaacg tccaggttgg cacgatgcag
     2401 atgcgctacg cgatgaaacg cgtgctctac cgcgcgatac tccggcgtgc ctcgcgttgg
     2461 tccttcgact ccgctgtagc agttcaggtc cacgacgaag gaaactttat ccggcagcat
     2521 caagggcagg acggtaactt cgacaggcac acgctggtcc agtcccgcct ccgaccggac
     2581 gcgcagctcg cccgtatgca caccgggctt cgcatcatgc gggatataaa tatcgacgaa
     2641 cacgggctgc accgtctgac ccggcaccgg gttatctgtt gacggaatgg cgaagggcct
     2701 cgtcagcggc accagtggat ccggatggaa gaggttcggg ttcttgtcgt cccggacgaa
     2761 ccactcgcga tagagctgta tcgccccggt cttttgcagg actgcgggca gcttattgcc
     2821 tgcaaacagc ggcttggaga cttcaatcgt aacgccgttc tgcacaccct ccacggccaa
     2881 ctgaaacgct acgaactcat tgcgcgcacc ggtcagcttc acaccgtcgt tccagacgac
     2941 gtcgcgttcc agcagtgcac cgctcaccgg attgattttg accgtatccg gcgccgtcca
     3001 cacgcgggac cgcggaccgt cgctgcccgt caccggatcg gcagccggca gtttgcggct
     3061 gagaggcgcg tcgatgcgaa ccgggccaac ctctgtcacc gggcttgccg taccgcgttc
     3121 atccaaagta cgcgaagcaa aataataagt gcccggggcc aggtcgtcca gcttgaccgt
     3181 caccgttcca ctgaggctgt tgctcgtggc cagcggatgc ggtttcggag cgagaggatc
     3241 gatcagccag cgcggatagg ctttcgccgc ggcgaagtct ttgatgggag attgcgcata
     3301 tcgcacatcg agacgcgcac gcgcgccttc aaacaccagc gtgccgcccg catggcggcc
     3361 tcgcggagcg gcgggcacct tcgcgttcgg cttatccgct tctaccacga gtaccggcgc
     3421 gtgcttccct tctttgctcg acagcacgtg ccgcgtccgc gtctggcctt tttcgtccgt
     3481 cagcaggata ccgaactggt cgccagtggc cagcgccgtc acgatgtcgg gtgagacatc
     3541 cgcctcaaac cagccattcc cggcgtcgcg cacacgcggg tacacataaa ggctgccgcc
     3601 ctgggagaac gtgacgtcca ccaggtccga ccctttgtat gaccaccaac cggcaggcga
     3661 cgggcggtag aagatcgcct cgctttcctt ccacgggccg tttccgctca cggttgaaat
     3721 gccgaccgta tgcaatggaa ccggtgatgg agcggcatgc acgcgcagca tcgccttggt
     3781 tacctggagc cctttcaggg cagacgtgtc gaactggagc agcgcaaagc tgacccggcc
     3841 ctggatcacc aactccggtt ccgccccgcg aggcggctcc ggtttcgacg ccaggtcggc
     3901 aaaggtgacg gtcgcgtcgg cagtgaccgg gagcgtaagg cggtccgcgc ccatcgcgca
     3961 aaacgcggca agcagaagag tgacgaattt cattccattc gagtttatcc aacctggtat
     4021 ccaatgcagg agttgttacg atgactttat gggtcctact cccgcaagcg aacaacctaa
     4081 acttccgatt gaaggcgttc ggaacctgat tgcgatcggt tccggcaaag gaggcgtggg
     4141 gaaaaccacg gtggccgtga acctcgcgct tgcgctgcgc agcctgggat cgaaagtcgg
     4201 attgctcgat gccgatgtgt atggccccaa tgttccgctg atgatggggt tgcaggagac
     4261 gccgcacgct attggcaacc ggattcagcc gctcgaaaaa tacggaatca agctgatgtc
     4321 gatgggcttt ctgaatcccg gcgacaaacc tctgatctgg cgcggtccca tgctgcatag
     4381 cgtgatgcag cagttcctgc gcggcgtgga ttggggcgag ttggattacc tgctgatcga
     4441 cttacctcct gggacaggcg acgtgcaact ctccctgatc cagacggccc cactgacggg
     4501 atcgattgtg gtaacgacgc cgtcggaggt atcgctcgag gatgcccgaa aagccgtgaa
     4561 catgttccaa caggtaaggg tggaagtgat cgggctgatc gagaatatga gttacctgaa
     4621 gtgcccgcac tgttcggaac ggatcgacgt tttcagcacc ggagggggac agaagacggc
     4681 gcaggcgatg ggtgtgaatt tcctcggtgc gctacccctc gacccgcagg ttcgtatcgg
     4741 gggcgacacc gggaatccgg tggcactgaa agactcctcg gcatttcatg agatcggcgc
     4801 gcaggtattg gaacgagtgc aggcggtcgg agccccgcgc ggcccctcca tcgaaatcga
     4861 agattagcag gcgcaggagg ccggctcgca aagaggccgt cctggccgga ggccctctcc
     4921 cgcgagggcc attggatgcc aaaaagaggc gaaccggcga taaaaagagc ccattcgatc
     4981 tgattctctt gaaaaacgct aatctttata tgagattagc gatcatgggc atcaacatcg
     5041 gtctggatat cggcgccatc agcatcaagc tcgccgcgct cggcaaaccc gaagaccggc
     5101 ccattgtgga ccgtctggga cagcctgggt cgagttttcg acggattgag gacgtgtgtg
     5161 gcaatccggc ggcaatctcc gaataccgcc ggatatccgg cagtcccatt caatccgcat
     5221 acgatctgct tctggatctc tacgaatttg tcccggagcg gttgattgaa ggaatccggg
     5281 tgaccggatc gggcagccgc accatcgcaa aaatactcgg tatctacttt gagaacgagt
     5341 tcaaggcgat cgcgcgcatg tttgccgcgg tgcacccgga ggtgcgaagc attttcgaat
     5401 tgggcggtga aagctccaag tatatccgtc tcgaaccagg ggacgattcg ggcggctgcg
     5461 gcattctgga ctacgaccgg agcggggagt gcgccgctgg aacaggctcg tttctcgacc
     5521 agcaggcgct gcggatgggc tactcggttg aggaagtggg cgacgtcgtc tcgtgcgcgg
     5581 gttgtggagc gcggatcgcg ggccgatgtt cggtgttcgc caagtccgac atgattcatg
     5641 cccagcaaaa gggctattcg cccgccgaaa ttctgcgggg cctttgcgac gctgttgcac
     5701 ggaacttcaa aagcagcatc gtgaagagcc gggccatcgc tcctccggtg gcgctgatcg
     5761 gcgcggtttc gcaaaacgcc ggaattgccg gtgcgctgcg ggaggtgttc cgcctgggcg
     5821 agagcgacct gatggttcca caactctacg catggtgcgg agcgttgggc gcagcgattc
     5881 tcgaagccga ggagccccga aaacggtcgt tccgcgatat tcacaagttg aaccagcacg
     5941 aatcggtaat ctgtcgcctg gatagcaaac cgctctcgat ggagaaggtg gttctgcttg
     6001 gagaccgtgt cgataaatac cagccgcccg aaggcgatgg tcccatcgcg gcgtatctcg
     6061 gaatcgatat cggatccgtt tcgaccaacc tggtcgccat cgacgagtcc gcgaacgtga
     6121 tccacgatat ttacctgcga accgccggca ggcccattga agcagtgcag aaaggactga
     6181 cggagttgaa ggagctctgg ggccaccggt tggtcattga gggcgtgggt accaccggct
     6241 ccggacgcga gttgatcgcg gagttcgtgg gcgccgacgt cgtcaacgac gagattaccg
     6301 cccacaaaac cggcgcaatc cacatcagcg acacgctggg aggagagccg gtcgatacca
     6361 ttttcgagat cggcgggcag gattcgaaat acatctcgat cgagaacggc gtcgtggtgg
     6421 atttcgcgat gaatgaggca tgcgcggcgg gcaccggatc gttcctggaa gaacaggcgg
     6481 agaagctcgg tatcagtatc aaaggggagt ttgcgcggct tgctttatcg tcccaggcgc
     6541 caacccgcct cggtgaacgt tgtacggtat tcatggagcg cgatgtcacc ggctggcttc
     6601 acaacggcga gacggtgccg aacctggtag cgggactggc gtattccatt gttctgaact
     6661 acgtgaaccg cgtggtccgc gggcggaaga tcggcgatgt gatctacttc cagggtggaa
     6721 cggcttataa tgacgcggtt gccgcggcgt ttgcgaaact gctgggcaag aagatcaccg
     6781 tgccgccgta caacggtgtg atgggggcgg tgggaatggc cctgatcgcg cggcaatggc
     6841 atcgggcgac gcggggattc acacgatttc ggggctatga tctgaataac ctggaaatgt
     6901 cctcacggga ctttgtctgt aaagcctgct cgaacgagtg cgacatcaag gaattcaaga
     6961 tcgaaggaca gaaaagctac tggggtgata agtgttcaga ccgttaccgg aaggtctcct
     7021 cgacgggccg taagccggta atcgaagatc tgtttgcgtt ccgcgaaaaa ctccttgaag
     7081 agataacggg tgccccgctt gcgcctccga aacaactgcc gcgatcggac cggatctgcg
     7141 tcggcattcc gcgcacgatg cccacctatg atcagtatcc gttctggcag cattacttca
     7201 ccgccattgg cgtcgacgtg gttctgtcgc ggcaaacaga tcccaaaatt gcggcggacg
     7261 gtgtggaaat ggccctggcg caaccctgct acccagtgca gattgcccat ggacacgtgc
     7321 agtcgctgct cgacctgggt gtggattacg tgctgacgcc gaacattctg aacgcggagg
     7381 cgcacgaaga caccgtatgc acttcgcata tgtgcccgtg gaaccagacg ctgccttttg
     7441 ttctgcaatc cgcgccccgg ctcgaaccgc accgccacaa gttccttacg cctactctac
     7501 actttcagct tgggcgccaa tacgtagccc ggacgctcgc ccaaatggcg cagaaactgg
     7561 gagtgaagcg gcgcgtgagc gacgcggccg tggatgcggc ctatacggcc caaagacagt
     7621 tccaggaacg aattctcgaa gcgggagccg ccgccatggc ggcgctcgat gagagcgggg
     7681 agcccggcat tttgctcatc gggcggggct ataacatcta cgaccgcaac atcaactgcg
     7741 acattccgag aaagctgcgg caccactacg gagcgaatgt cattccgctt gattttctcg
     7801 tcacaggacg ggagagggtg caggatctgc atcccaacat gtattggtcc tcgggccaga
     7861 agattctggc cgcgtcccgt ctggcggcgg cgcatcccaa cctgcacatc atctacatca
     7921 caaacttcaa gtgcgggccg gattcgtata tcaagtattt cgcgcgccag accgccggtg
     7981 tcccgttgct ggtgttgcaa ttcgacgggc acggaaacga cgcgggctat atgacccggt
     8041 gcgaagcgta tctcgacagt aaaggaattc tgcgatgcta cagcagccaa ccctcgagcg
     8101 aaaaagcgat ccgcgcggca gccacccact gaccggcaag acggtttata ttccgccgat
     8161 ggcctacggc agcgcgcgcg cgttcgccgc aggattccgc gcgctgggcg tggacgccag
     8221 gctgacgccg ccttccgacg aacgaaccaa agaactcggc gggcgctata catcgggtga
     8281 cgagtgttat ccggccaaag taaccgtggg cgattttatg cgggtgttgg agcaaccctc
     8341 caccgatcag gtcaagaccg tgttttttat gcccaccgcg gacggaccgt gccgttttgg
     8401 ccaatacgcg acctatctcc ggcgcattct cgacctgaac ggattcccca gtgcgcaggt
     8461 gctctcaccg accagcgcca acggatacga agggctgggc aatctcgcgc ggccattttt
     8521 ccgcacgggt tggcgtacac tcgtggcggg cgatattctg caaaaactgc tgctcatcta
     8581 tcgtccttac gagacgcggc gcggggaaac ggacgaggtg taccacgtgt gcctcgacga
     8641 tgtgtgcggc acggtggagc gaaccacggt ggagccttcc gtgcaactga aggcgattcg
     8701 cgaagcgctt ttgcgatgcc gcggccgctt tcatgccatt tcagcgcgcc gcgaccggtc
     8761 cgtcccgctc atcggcgtgg ttggtgagat cttttgcagg ctcaatacgt tttcgaatga
     8821 cgatctcatc cgccgcctgg aggaatatgg cggtgaggcg tggctctccg acatcggcga
     8881 atggatctgg tatacgaacg cggagcagtt ccggcgcttg aagctgctcg gcaaacagat
     8941 ttcgaccgac gccgccaaag cgtgggtccg cagcaagata caacggcacg acgaacatgt
     9001 gctgctcgag ccgttccgtg acgactttcg aggatacgaa gagccgcccg ttgaagagat
     9061 tctggagtat gcgcggccgt acctgccctg cgacggcgcg ctcggagaga tggtgctcaa
     9121 cgtcggaaaa gccatctacc ttgccaggaa aggcgccgac ggcatcctgg acatcagccc
     9181 gttcacgtgc atgaacggca tcgtctgcga agccatctat ccccggttga gccgcgacct
     9241 tggcggcatt ccgatccgca cgttctattt cgacgggaca caagccgacc tggatcgcga
     9301 tctcggggtg tacctggaac ttgcacgttc ctacagccgg aaaaaaagta ccgcgcgcgt
     9361 ttaccccgac cgtccctgac caattccgcg gtgccgctgt cgccgggtta ccaaatagtg
     9421 gtgagttgtg ccgcgcgaat tttcgaatcc cggctgatga gtggaacacc caaggccaca
     9481 gcagtggcag cgatgatgcg gtcgggaagg tccggcacag attcccggct gaccgtggat
     9541 acagcctcgg caacctcgag tgtcaacggt tgctccacaa aggttgagtt gccctcccga
     9601 atcgcagaaa tcagtcttgc ccgagcttcc ggatgtagct tacccttctc aatcaggtag
     9661 gttaattcca ccagacaaat tgttggcacg aatacctggt agggaggctg ctgagcgcca
     9721 tcgatggcct gcctggcgct gatcgatagg ctcgggtggg cagcaagata ccaaacggcg
     9781 gcatgcgtat cgagcacgac gcttggcatc aaacgtcgtc ccgcaggaaa ttcttccagg
     9841 cctcatgccg tacttcatcg atctccgccg cagacggacc tggccctaga tctttccaaa
     9901 gcccgtataa gttacgaaca ggcttctcga ctccgttcgg ctcttggagc gattcggcgt
     9961 atcgcagcac cgcctccttt ttgtttgggg gcaaggcgcg taccgcttgc acaatcgact
    10021 cttcgaccgt catatcgaac ctgtatctac tattatcagc cccacggcct ccaagaggaa
    10081 gctcaggggg cggtgtgacc tcgtcgctgg gccgcatcag tttctacgcc gacgggcggt
    10141 tcgtggctga accaaaagcc agccgtgaga agccgggaca ggatattttg agtaagctgg
    10201 aaagctacag cctcgatttc cgcaagatgc gcttctcggc cgaagcctca aatcgtcaga
    10261 cgccgtctgg ccggtaggac cgggttgggc ctggcatgtg ctggcattgg gcaatttagc
    10321 tttggaaggc tcgcgttata atcggggaag ggttaaatag gccctaaggg ggacaatgag
    10381 caaagaagac agtattgaag tgacaggaac tgtagtggaa aagtttccga gcggactctt
    10441 cagcgtgcaa ctggaccagg agcgtaccgt gctggcgcac ttggcgggca agctgcgccg
    10501 caatcgtatc agggtactgg cgggtgaccg cgtaacgatg gaactctctc cgtacgactt
    10561 gaccaagggt cgaatcacct accggcacaa atagaacctt ccttttcctc ccgcgcgtgc
    10621 cgaaaatcct catcgtattc gggacgagac ccgaggcgat caaactcgct ccggtcgtaa
    10681 aagagcttcg ttcccgctcc agccattttg atgttcaggt ttgcataacg gcgcagcatc
    10741 gtgagctgtt ggaccaggtg ctcgaggtct tctcgataga gcccgagttc gacctcgacc
    10801 tgatgcttgc cgggcaaacc ctgtttcagt cgacctcgcg cgtggtggcc gggctcgaat
    10861 ccgttctcca ccaggcgcgc ccggacatgg tcctggtaca aggcgacacg acgacgacaa
    10921 tgtgcggcgc actggcggcg ttttacgctc gcgtgcccgt gggacacgtg gaggccgggc
    10981 tccgaacggg aactccgggc attccttttc ccgaagagat gaaccgtgtc ctcaccagcc
    11041 gactggcatc gcttcacttc gcggctacgg agtgggcggc ggcaaatctg cgcaaagaag
    11101 gagttgcgcc cgaacggatc gtggtgactg gcaataccgg aattgacgcg gtgctgcacg
    11161 tccatgccgg aattgaatcg gggcggctgg ttccatgccg gaatattccg gttgatcccg
    11221 gcaggaagct gattctggtg accgcgcaca gaagggagag cttcggagaa ggcttcgagc
    11281 aaatctgcgc ggccctgatc gagttgtcgc ggcgcccgga tgtgaccatc gtttatccgg
    11341 ttcatcccaa tcccaacgtg caggaaccgg tcaaccggat tttggagggc gccgaacgga
    11401 ttctgttggt tgagccactc gactatgtct cgttcgtcgg cctgatgcgg cgcgcctatc
    11461 tgctgatcac ggactcgggc ggcgtgcagg aagaggcgcc ttctctggga aagccggtgc
    11521 tggtgatgcg tgcctccacg gaaaggccgg aggcagtgga agcaggcacg gcacgcctgg
    11581 tagggacgga cgcgcgggcg atcgttcgag aggcgtcgcg gctgctcgac gacccggaag
    11641 agtacgcttc catggcacgc gttcacaacc cttatggaga cggccgggcg agccgtcgta
    11701 tcgcggacgc aatactctca ttcttcaagt gaaaacgctt cccggcctgt gtccgccaag
    11761 gcatttccga ggcggccgga accccggaga agccagggga caaccgctat aatcaggccg
    11821 gcgaggaaca cgggcttaat gagaaatttt cgcggcgggt cgaaatcaaa aaaagagacg
    11881 cctggcactg tctcaggact gaaggcaggg ttgcacaagc gcatcgtggg gcagccggca
    11941 gccctggaag caatcgtacc gcacatacag atccatcaag ccggcctgtc accggaggga
    12001 aggcccgccg gcgtgttcct cctgctgggg cccactggca cgggaaagac gaagacggtg
    12061 gaagcgcttg ccgaggtgct gcatgggagc gagcgaaacc tgctgcgaat cgattgtgcc
    12121 gaatttcaac tggatcacga ggtggcgaag ctgatcggtg cgcctcccgg atacctgggc
    12181 catcgtgaaa cgcacccgct gttgacgcag gcgaagctga attccgtcgt aagcgaacgc
    12241 agtccgcttt cgatcgtgct gttcgacgag attgagaaag ccgcaccgtc gttgacgcgc
    12301 ctgctgctcg gcgtgctgga caaagctacg ctgcggctcg gcgacaacac ggcagtgaac
    12361 ttcgagcgga gcctgatctt cctgaccagc aacctgggcg cgagagacat ggccagggaa
    12421 ttgagccccg ggttcgggtt tgatgcaatg gccggttccc gtccggcgtt gagcggcaag
    12481 ctggaaaacg tagcgatgcg cgcggtccgg aagcattttt ctcccgaatt cgtgaatcgc
    12541 ctcgatacag tggtcacgta tcagccgctg gacgaaaagt ccttgtcgga gatcctcgac
    12601 ctccaactgg cgggattgca gcaacacatt cattcgcggc gtggctgcga aacgttctat
    12661 ttgcacgtgg cggcttccgg acggaaattt ctcctgaatg cgggtaccag cccggagtac
    12721 ggcgcgcggg aattgaaacg cgccatccaa cgccatctga tcacgcccct ggccgaactc
    12781 atcgagttgg gaattgagcc cggcgcgaca gtcatcgcag atgcccccaa aaacagcacg
    12841 cgactgcatt tccggatgcc ggcaatccgg ttggctgcat tggatgcggc ctgattccag
    12901 cgtcagcaca catcggcaca ggaacgaagc ccgttggagc gttctgctac catagcgctt
    12961 ggcaatgggg gcttcggagg agcggcagtc cggattacgc cgtgaaatcg gccttcgcga
    13021 cctcgtcctg ttcaacattg cggcggtaat cggaatccgg tggcttgccg ccgcggcgca
    13081 tgcgggtccg ggctccatct ccttgtggct gctcgcggcg ctgctgtttt tcgttccctc
    13141 cgcactggcc gtttccatgc tgtccaggcg cttcccggaa gagggcggcc tctatatctg
    13201 gacgaagcgt agtttcggcg actggcacgg cttcgttgca ggctggtgct tctggctgtc
    13261 taatcttttc tatttcccga atctggcgtt ggccggcgtc gcaatgggcg tctatacttt
    13321 gggcgcccaa cacacgcatc tggcaagcaa tcgcggtttt gttctgggcg cgagcctggt
    13381 gctgctttgg gcagcgctgc tgagccacct tttcggacta cgaatcggca aatggaccca
    13441 gaacatcggc ggcctttcga cgtatgccgc gggcgggctg atcatcgccg ccggtgccat
    13501 tgtctgggcc ttgtatggcg ccgccactcc gatcgaaatg gccccccaat ggaactggga
    13561 caaggtcaat ttctggtctc agatcgcctt cgcattcggt ggtttggaac tgggcgccgt
    13621 gatgggcgga gagatccgta atccgaagcg gaacgttccg cgggcggcgt ggatcagcgg
    13681 ctccgcgatc tgcgtgttct acattgcggg cactctcgcc attctggcgt tgcttgctcc
    13741 tgagaatgtc agcgtgctta cgggcctcgc gcaagcagga caggttgcgg ctgaacggac
    13801 gggcctcgcc tggttttcgc ccctgctcgc cggtctcatt actttgggag tggtgggaca
    13861 actcggggcg tgggtgggcg gcagcgcgcg cgtccctttc gctatcggac tggatcgcta
    13921 tctgccaccc gcactcggaa aactgcatcc acgctggggt acgccttaca tctcccttct
    13981 ggcacagggc gtcgtttgca c
//
LOCUS       DASTMK010000425         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_84760, whole genome shotgun sequence.
ACCESSION   DASTMK010000425 DASTMK010000000
VERSION     DASTMK010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84760"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFQ79_18300"
     CDS             complement(<1..681)
                     /locus_tag="VFQ79_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141681.1"
                     /translation="MSTEPFLSVIVTITSGAPNLGRCLKALEAQRNAPPMEILVPVYE
                     NFDEVAALRTAYPAVCFLPVEGDQEGLASGDPGLLHLTYDRRRATGLAAARGEWIAMT
                     EDHAIPGETWCAGIAEAHRKSYAAIGGAIENASPRWLTRAAYFCDFGRYANPLKEGPA
                     HYASDINISYKRAAIEKTQPVWRRYYHETAVHAALEAAGETLWLTPRIVVRHDRGPLR
                     FGPLLRERF"
     gene            complement(691..1674)
                     /locus_tag="VFQ79_18305"
     CDS             complement(691..1674)
                     /locus_tag="VFQ79_18305"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU0141682.1"
                     /translation="MSWKHANVFVTGATGLLGSWLSEELVRRGARVTCLMRDWVPASR
                     LLASEAFSRMNVVHGELEDHALLVRALNEYEIDTVFHLGAQTIVGTASRSALSTFETN
                     IRGTWNLLEACRQCSKLMRRVIVASSDKAYGSHEKLPYTEQAPLEGRFPYDVSKSCAD
                     LITLSYWHTYRTPVAITRCGNLYGAGDLNFNRLIPGTIRSALANESPVIRSDGKFIRD
                     YFYVRDAVDAYLTLAEKMQDGRLEDRAFNFGTEVPASVLGVVETILALMKRTYLKPAV
                     LNEATHEIPRQYLDCTLARQLLGWRSKYTLEEGLAETIEWYSAPLQRAAVA"
BASE COUNT          323 a          558 c          543 g          355 t
ORIGIN      
        1 aaaccgttcc ctcagaagag gcccgaaccg cagcgggccg cggtcgtgcc gcaccacaat
       61 ccggggcgtc agccagagcg tctcgccggc ggcttcgagc gccgcatgca ccgctgtttc
      121 gtgatagtac cgccgccaga caggttgcgt tttttcgatc gccgcacgtt tgtacgatat
      181 gttgatgtcg ctcgcgtagt gcgccggacc ttctttgagc ggattggcgt atcgtccgaa
      241 atcgcagaag taggcggcgc gggtcagcca tcgcgggctt gcgttctcaa tcgccccgcc
      301 gatagccgca taggatttgc ggtgggcctc cgcgatcccg gcacaccagg tttcgcccgg
      361 aatggcgtga tcttccgtca tcgcgatcca ttcaccccgc gccgcggcca gaccggtagc
      421 acgccgccgg tcgtaggtca ggtggagcag gccagggtcg cccgacgcca gtccctcctg
      481 gtcgccttcg acaggaagga aacacaccgc cgggtatgcg gtgcgcaagg ctgctacctc
      541 gtcgaaattt tcataaacag gaaccaggat ttccatcggc ggtgcgttcc gctgggcttc
      601 gagcgctttg agacatcggc ccaggttcgg cgctccgctg gtgatggtga caatgaccga
      661 taaaaagggt tcggtactca caacctcaac tcaagccacc gcggcacgct gcagcggcgc
      721 ggaataccac tcgatggtct ccgccagccc ctcttccagc gtgtacttcg accgccaccc
      781 gaggagttgt cttgccagcg tgcaatccag atattgccgg gggatctcat gcgtggcctc
      841 gttcagtacg gccggcttca ggtacgtcct cttcatcagc gccaggatgg tttcaaccac
      901 ccccagcacc gatgccggca cttcggtccc aaagttgaat gcacggtcct ccagccgtcc
      961 atcctgcatt ttttcggcca gtgtcagata cgcatccacg gcgtcccgga cgtagaagta
     1021 atcgcggatg aacttgccgt cgctgcggat gacgggcgat tcgttcgcca gcgccgagcg
     1081 gatggtgccc ggtatcaggc gattgaaatt caggtcgccc gcgccgtaga gattgccgca
     1141 gcgcgtgata gcgaccggcg tgcggtacgt gtgccagtag gagagcgtga tcaggtcggc
     1201 gcaggacttt gacacgtcat aagggaagcg cccctcgagt ggagcctgct ctgtgtaggg
     1261 caacttctcg tgcgatccgt aggccttgtc gctcgaggcc acgatgacgc ggcgcatcag
     1321 tttcgagcat tgccggcagg cttcgagcag gttccaggta ccgcgaatgt tcgtctcaaa
     1381 cgtggaaagc gccgagcgcg acgccgtgcc cacgatggtt tgtgcgccca ggtgaaacac
     1441 ggtgtcgatt tcgtattcgt tcagcgcgcg taccagcagc gcatgatctt cgagctcgcc
     1501 gtggaccacg ttcatgcgcg aaaacgcctc cgaagcaaga agacggctgg ctggtaccca
     1561 atcgcgcatc aggcaggtaa cgcgggctcc tcgccggaca agttcttcgc tcagccacga
     1621 tccgagcagg ccggtggcgc cggtgacgaa tacgttcgca tgtttccagg acattgttat
     1681 tcccatatct tccagggggc gtttcctccg gcccataacg actcgagcaa ctgcttttcg
     1741 cgcagcgtgt ccatcggttg ccagaagccg gtgtgccgg
//
LOCUS       DASTMK010000426         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_117662, whole genome shotgun sequence.
ACCESSION   DASTMK010000426 DASTMK010000000
VERSION     DASTMK010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117662"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFQ79_18310"
     CDS             <1..379
                     /locus_tag="VFQ79_18310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0141683.1"
                     /translation="ELPAGGFDGIFCGQVVEHIAPERLPELIRLMARALQPGGVLVVE
                     TPNPESLAIFATHFYIDPTHVRPVPAALLVFYMEEYGLGGIRVERRFPAVASSPALAQ
                     LPEEVRDSFFGGLDYAAIATKLT"
     gene            423..1181
                     /locus_tag="VFQ79_18315"
     CDS             423..1181
                     /locus_tag="VFQ79_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0141684.1"
                     /translation="MAAGKLAGRNALITGASKGLGKAMALALGKEGANIALVARDTDR
                     LAAVAEEIRANGSDAESYSLDVSNEAAVRELEAAALARFGHIDILINNAGINLRKPVT
                     EFTLEEWNQVVSVNLTSAFLLCRSFVPQMKGRGYGRIINMTSTMGHVSLPGRTAYSAT
                     KAALIGFTKALALELANEAITVVGISPGPFATEMNLPLLQNPEVNQQFIANIPVGRWG
                     RVEEIGALAAFLCSDEAAFLTGTDILIDGGWCAR"
     gene            1171..2142
                     /locus_tag="VFQ79_18320"
     CDS             1171..2142
                     /locus_tag="VFQ79_18320"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0141685.1"
                     /translation="MRASRWLLLVLGVALAVSSWGCRRDDRKVIAVIPKSTSHLFWVS
                     VEAGARSAGRDLNVEVLWNGPPAETDYSRQIQIMDSMIAKRVDGIALAASERKALVGP
                     LERAAASGIPVTVFDSGLDSEHYLTFVATNNYEAGQMAARELARLIGGKGTVGVILHA
                     PGSYSTMDREQGFQDLIAREYPQIKIVAQQYGMADPARSRAAAENILTAYPRLDAMFA
                     STEPSSAGATLAIKARGLTGKIKFVAFDSSENMIQDLKSGAIDAMVVQDPFRIGYEAV
                     QTLVDKLNGKQPPKRMDLSAQLIRREDLERAEVRQLLFPDVRTHLRQ"
     gene            2139..3179
                     /locus_tag="VFQ79_18325"
     CDS             2139..3179
                     /locus_tag="VFQ79_18325"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU0141686.1"
                     /translation="MTNDVTISSHDLIAFGSKLLEATGVTPERAHLVAASLVAADLRG
                     VDSHGIQSLPFYIRQIEHSSIRIDAEGHVLSETGGLALYDGENGLGQVVSQRCTELAI
                     RLGREHGFGVVVARESNHFGAAAYWAQQISAEGFIGIVMCDASHVVPPWQGREGRIGT
                     NPICMSVPGEEWLLDMATTTVARGKMLKAVLRGDKEIPAGWALDKDGVPTTDPEAAMA
                     GLPMPLGGYKGSGLAMMVEILCGVLSGGAMATQVGGIHFYTQPARISQFFLALDVSRF
                     MPLDQFRARVAHLVAEVKSAPPALGYDEVLVAGEPERRVESQRCREGITIPAGVWAKL
                     TENARKWNVPVP"
     gene            3183..3749
                     /locus_tag="VFQ79_18330"
     CDS             3183..3749
                     /locus_tag="VFQ79_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HEU0141687.1"
                     /translation="MNSNKKNLSPEQRELLLRTLQARFEKNRNRHKGLEWAQVQTRLK
                     ANAEELWSLHEMERTGGEPDVAGHDEKTGEYIFYDCSAESPKGRRSLCYDREALESRK
                     QHKPENNALDMAAAMGIELLTEEQYRELQKLGEFDTKTSSWIKTPSRIRKLGGALFCD
                     RRYDTVFVYHNGAESYYAARGFRGLLRV"
BASE COUNT          799 a         1105 c         1217 g          717 t
ORIGIN      
        1 ggaattgccc gccggcggtt tcgatggaat cttctgcggc caggtagtgg aacacattgc
       61 gcccgagcgt ctgccggaac tcatccggtt gatggccagg gctctgcaac ccggcggagt
      121 gctggtagtg gaaacgccga acccggaaag cctggctatt ttcgccacgc acttctatat
      181 cgatccgacc cacgtacggc cggtgcccgc tgcattgctg gttttttaca tggaggagta
      241 tgggctgggc ggcatccgcg tagaacggcg attccccgcg gtggcatcgt cacccgcgtt
      301 ggcccaattg cccgaagagg ttcgcgatag cttcttcggc ggcctggatt acgcggctat
      361 tgccacgaag ctcacttgag acgctccggt acaatagcca gattgaggag aaattccgat
      421 taatggcggc tggaaaactg gcggggcgca atgcgctgat cacgggtgcg agcaagggac
      481 tgggaaaggc catggcgtta gcgctgggca aagaaggcgc aaacatcgca ctcgtggcac
      541 gcgatacgga ccggctcgcg gcagtggcag aggagattcg cgcgaacgga agcgacgccg
      601 aatcctactc cctggatgtg tcgaatgaag ccgctgtgcg ggagctcgag gcggcggcgc
      661 tggcgcgctt cggccacatc gacatcctca tcaacaacgc cggcattaat cttcgaaagc
      721 ccgtgaccga gttcacgctc gaggagtgga accaggtcgt cagcgtcaac ctgaccagcg
      781 cgtttctcct ctgccggtcc ttcgttccac aaatgaaggg acgtggatac gggcggatca
      841 tcaacatgac atcgacgatg ggccatgtct cgctgcccgg acggaccgcc tactcggcca
      901 ccaaggcggc gctgatcggt ttcaccaaag ccctggcgct tgaactggca aacgaagcga
      961 ttaccgtggt cggtatcagt cccggcccgt ttgccaccga gatgaatttg ccgcttcttc
     1021 agaacccgga ggtgaatcag cagttcattg cgaacatccc cgtcggccgg tggggccgcg
     1081 tggaggagat cggagcgctg gcggcgtttc tctgctcgga cgaagctgcc tttttgaccg
     1141 ggacggacat cctgatcgat gggggatggt gtgcgcgcta gccgctggct tctgctcgtt
     1201 ctgggcgtcg ccctggcggt aagctcctgg ggctgccggc gcgacgacag gaaggtgatc
     1261 gcggtgatcc ccaaatcaac ttcacacttg ttctgggtgt ctgtagaggc cggcgccagg
     1321 tccgcgggcc gcgatctgaa tgtcgaagta ttatggaacg gtccgccggc ggagaccgac
     1381 tacagcaggc agatacagat catggattcc atgatcgcca agcgcgtgga tggaatcgct
     1441 cttgccgcca gcgagcggaa agctctggtc ggcccactgg agcgcgcggc cgcgtcggga
     1501 attccggtta ccgtgttcga ttccggtctc gactcggagc attacctgac gttcgtcgcg
     1561 accaacaact acgaagcggg ccagatggca gcacgtgaac ttgcgcggct gatcggtgga
     1621 aaggggacgg tcggagtcat cctgcatgct cccggcagct attccaccat ggaccgtgaa
     1681 cagggttttc aggatctgat cgccagggaa tatccgcaga ttaaaatcgt ggcgcagcag
     1741 tatggcatgg cggatcccgc ccgctcgcgt gccgccgccg aaaatattct tacggcctat
     1801 ccccgcctcg acgcgatgtt cgcctcgacg gaaccgagct ccgccggcgc gacgctcgcc
     1861 atcaaagcac gtggcctcac aggcaaaatc aagttcgtcg ctttcgattc gagcgagaac
     1921 atgattcagg atttgaagtc cggcgccatc gacgccatgg tggtgcagga ccccttccgc
     1981 atcgggtatg aagcggtcca aacactcgtg gacaagctaa acggaaagca gccgcccaaa
     2041 cgaatggacc tgagcgctca gctcatccgc cgtgaagacc tggagcgcgc ggaagtcagg
     2101 caactgcttt tccccgacgt ccggacacac cttcgccaat gaccaacgac gtaacaattt
     2161 cttctcacga tctcatcgcg tttggtagta agctgctcga agctacgggc gtcacgccgg
     2221 aacgggctca cctggtagcg gcatcgctgg tggcagccga tctgcgcggc gtggattcgc
     2281 acgggattca gtctctgccg ttctacattc gccagatcga acattccagc atccggattg
     2341 acgccgaggg ccacgtgcta tcggaaacgg gcggccttgc cctatacgat ggcgagaacg
     2401 ggctggggca ggtggtgtca cagcggtgca ccgaactggc gatccgcctg gggcgggaac
     2461 atggcttcgg cgtcgtggtg gcgcgggagt cgaaccattt cggagccgcc gcctattggg
     2521 cgcaacagat ctcggcggaa ggattcatcg gtatcgtgat gtgcgatgcg tcgcacgtag
     2581 tgccgccctg gcagggccgc gagggacgga ttggcacgaa cccgatctgt atgtccgtgc
     2641 ccggcgagga gtggctgctc gacatggcga ccaccacggt agcgcgcggc aaaatgctga
     2701 aagcggtgct gcgcggcgat aaagagattc cggcgggttg ggcgctcgac aaggacggcg
     2761 taccgaccac cgaccccgag gcggccatgg ccgggcttcc gatgccgctg ggcggctaca
     2821 aaggctcggg cctggcgatg atggtggaaa ttctctgcgg cgtgctgagc ggcggcgcca
     2881 tggcgacgca ggtgggaggc attcatttct acactcaacc ggcccgcatt tcgcagtttt
     2941 tcctggctct cgatgtctcc cgtttcatgc cgctcgacca gttcagagca cgggtggcgc
     3001 acctggtcgc agaagtgaaa tcggcgccgc cagccttggg atacgacgag gttctggtgg
     3061 ccggcgaacc ggagcggcgc gtggaatcgc aacgctgccg cgaagggatt accatcccgg
     3121 ccggagtgtg ggccaagctg acggagaacg cccggaaatg gaatgtgccg gtaccgtgaa
     3181 gtatgaatag caacaagaag aacttgtcgc cagaacagcg cgaactgcta ctcagaacat
     3241 tgcaggcccg tttcgagaaa aacaggaatc gccataaagg tcttgaatgg gctcaagtcc
     3301 aaacaagact gaaagctaac gctgaagagc tctggtcgct gcatgaaatg gaaagaaccg
     3361 ggggtgaacc ggacgttgcc ggccacgacg aaaagacggg cgaatacatc ttctacgatt
     3421 gttcagcgga aagtccgaaa ggccgcagaa gtctttgcta tgaccgggaa gcgctggagt
     3481 cgaggaagca acataaaccg gagaataatg cgctcgacat ggcggctgcc atgggcatcg
     3541 agctcttaac ggaagaacaa tatcgggagc tgcagaaact cggagaattc gacacgaaga
     3601 cctcgagctg gatcaaaaca ccttctcgaa tcagaaaact cggcggcgcg cttttttgcg
     3661 atcgccgcta cgacactgtt ttcgtgtatc acaacggcgc ggaatcctac tatgccgcca
     3721 gagggttccg tggcttgctc agggtctgag ttgcacgtgc ctatctggtc atcaccgcat
     3781 cacacgcccg ggccagatat tcttcgtact cccaggatcc gctgccgccg gtctggcg
//
LOCUS       DASTMK010000427        11171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_181470, whole genome shotgun sequence.
ACCESSION   DASTMK010000427 DASTMK010000000
VERSION     DASTMK010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181470"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..826)
                     /locus_tag="VFQ79_18335"
     CDS             complement(380..826)
                     /locus_tag="VFQ79_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141688.1"
                     /translation="MPMELTERFVQERQYIINVSPKTVAWYREAFRQFATVGLNAVNE
                     QPAATLVANIKTRIMELIGSGKVKPISINCWLRVVNAYLRWAANEGHLSGALDSDGRL
                     LKRVKIPKLKEPKIVIEPLSKDDIAKLVKCSPKYKAEKRVHTTSVR"
     gene            complement(888..964)
                     /locus_tag="VFQ79_18340"
     tRNA            complement(888..964)
                     /locus_tag="VFQ79_18340"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(928..930),aa:Val,seq:tac)
     gene            complement(1000..2901)
                     /locus_tag="VFQ79_18345"
     CDS             complement(1000..2901)
                     /locus_tag="VFQ79_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141689.1"
                     /translation="MRPTILVLLFALAASAEQFSIRLIGQAQPNCWDVADIVSSVTKG
                     AGSEREKVLALHKFGMAHQIHFNGPIEERGHYVDDPLKLMAVYGFSLCGNNSKAMTAL
                     YNEAGMRARTRSMPGHSVPEIWFEGKWNYIDTDMFGYVFLEDGRRIASVDELVSAPDL
                     FLKQKNPPDPYYPFDEKADMASVFRGAKPSRNHHPYSNAHTMNLALRTNESIEFYYRP
                     RNRYLLTELPEDLGVVYKDYWVVGPVRKGSLAWTDQPPAAYGNALVEYVPDLRSSTFA
                     KENPQRANVTVAAERGKPQLSAERPGEVASLVVEVNTPFVIVGEQNDLTNFEDNSDAV
                     AVKGLFWRKDRRDENRIYVSTDAGRTWNKVWENRYLGAIPFEVDLTRWVEGRYSYHLK
                     FEWVDRAGSNAVGLEGLAFRTWVEVSPMALPRLVSGRNTFQLQTAPRRTYYHESYWHK
                     GQNLRDQKVENLVFDTTSSQYRLQDPDQPGTLTFRLGPDAAAEELRFSVSARAAGPMN
                     RVALSLSLSEDGGATWRQLADFKPDPEHNQNQMWFNHVLRGVNLDSARTWLKVSTAGA
                     NVEKVIANTVLPVKPATESTIEVTHQWYEDAALKSATHIVANGEGYELETSASAIRNH
                     ALRLRAVAR"
     gene            complement(2912..4699)
                     /locus_tag="VFQ79_18350"
     CDS             complement(2912..4699)
                     /locus_tag="VFQ79_18350"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEU0141690.1"
                     /translation="MALKCPFAFLVCAAAFAQPGDLPVPPVPAGIQEEFLPVSGEMLQ
                     PSEADHQRGWLLYQRDRNFELLPNSRPAPGEALTRLRITATPGEMESRPFSLYALREI
                     PAIRPELRGPSWLKIEDVLFHPVQLRTKSMGGSSGEFDTTRIKRIGRFVRYPVFLRPV
                     TEQAVPAGSSRLYWVTASVPPDAAPGLYQTTLRMAQPGGAALEVPLEVRVLPFKLDDP
                     GVRFGAFLSGRKFEKGEWAMMKRYGMDALQWFWGSHPIRVQNDAGKVRLDFTEFDAFV
                     RGMQQAGMRGPIVPSLGNSWLGMYEIALARAFDLRLMKRDLGGREVTLMDMTDPRWEK
                     PYIEGLRQIFAHAKEAGWPPLALLINDEPTKFIMAYHPYRYHLLKKHFPEVPVYGVFF
                     EPRKDPGPLLHSSDIVVANRDLEHMSRLAREFGKRFWTYNNITADQSFGKCRLLYGQI
                     PAFYGSEAMFFWSWNYYINNPWNDFDGRGEANDPLPQSDADWVAVYPSVDGAEPVRTL
                     AFEAAREGIDDVRYLKTLENLVKRKDPSRWEALRAEIHRRQEAMFQGIVLDNRIYTDA
                     EFFVTTRNDDVEHLRDFAIREILKSLDLQ"
     gene            4781..7069
                     /locus_tag="VFQ79_18355"
     CDS             4781..7069
                     /locus_tag="VFQ79_18355"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HEU0141691.1"
                     /translation="MNNRFPMLFLVMAVLGVYAAAPPVSNRAPAPNDIGYLPADGAKV
                     GTNPPALAWLPEPHADAYAVQLARDEGFQKGVITIRRTPYVLYTHTATLRPGTWWWRY
                     ACLDARGNQSAWSQARHFTIAGDAQPFPRPSDELVRERIPKSHPRLMLRPEEVEMFRQ
                     ARLGPEKARWEELVKAAEEYLKAPLMPEPPPWTDGKWNAPEWRRNFQDTLRAVEKAET
                     LAFCYLLSGDKRYGEGARKWLLHFASWNPAGSTSMDVNDEAGMPILYLTSRAYDWAYD
                     ALSKEDREAMRKMVRFRGEEAYDWLHDKPFEQQAYNSHGGRMWHFLGEAAIAYYGEVP
                     EATKWLDYALSIFWGWYPSYGDEDGGWAQGYSYWASYVNRSTWWFDALRAGLLIDGTE
                     KPFYRNVGLFPMYVAPPNGSLTGFGDFAEAEPNARAAVPVSYFARMRGVPEWQWFAEA
                     WGKGEGETGPIGFIRSARPDPPKVTARPPSNWPQAKWFRGAGWVSLHSGMMNPAENVQ
                     VMMRAAPLGNISHSHADQNAIVLAAFGSPLLVNTGIRPWYGSPFSKEWYWTTKAHNAL
                     EIDGQGQPKTAEATGEIIVFQPGKEYDYVVGDATPGYGANMERYRRHLLFLKPDVIVL
                     VDEVSARKPVSLKFWLHGRAPFELNPAGDHVALTFGKASLSGFLRSPGGVQTVQTDKY
                     PLPPEQGQTRPEWHLSSETKAKHTNARFVAVLGVGKAGEKIPLDSVEDASSGGRIAVQ
                     FRRAGKPVSISIDPAGPTVSIR"
     gene            7074..8969
                     /locus_tag="VFQ79_18360"
     CDS             7074..8969
                     /locus_tag="VFQ79_18360"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141692.1"
                     /translation="MSSRVKPFLSLWIACTVLPAQTWIAATDEALVKRTGTWTAAEFR
                     RASSGHLLTVEDGAALELTFTGTRVVLSLDAHGLSYAHLGLENLGILEVTLDGQPSTA
                     VLPQQEDRDVVLARELRNGAHTLRLVHRATSAGSGARVAGFRILSGNEGGLSFLLHGE
                     ANRYLNDIRAVVSKNGQVVRNGLFRNWLTGQCRIAGLPAGPGYEVELMAAGWQTQHLR
                     NIEIKAGQETELRPVYLLRKPESTTSGVEFPRAGQPAIVRSGESFATRVFLAGASIES
                     VELRRQIGPATISREVVFREDKAREQDGRAEGIITLPTGVAPGLYDLVFRLSKKASRS
                     APRAVYVVAEYPKNPVFVTFGHMDTFGQEAAEYLQRLAGLSNLIGADMVLVSNEVNAA
                     YALGAFARLDMPHLITFGNHEVAGHEEFYGNAISMIDYGPELSILNFSHAWHGDLSHA
                     YALLDSRAKTRCKIINAFEHDAPVEAMLDRYGISFLHEAHGPDPKVMQIGRTPTQRAG
                     KVNSESFRVVRFEGCRPVSFTYAGHPSAPIPLPRHQPAPMQLSFSAPNDGTARRVTAR
                     ISNRWAQSFPHGRVVFVMPAGDCTVEGGRMESAIASDDGRFVVLTARVDIPANADIQV
                     TAESRRR"
     gene            8996..9916
                     /locus_tag="VFQ79_18365"
     CDS             8996..9916
                     /locus_tag="VFQ79_18365"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0141693.1"
                     /translation="MQRRSFLVSTAAAMLNAAPEQPRTFVYKTVPGCDIKADVYYGGK
                     AAAVWIHGGALISGSRRLSRDSRVLRSLLEAGFTVVSIDYRLAPETKLPEIIEDIRDA
                     FRWLRTEAGSLGIDGDRVVVCGGSAGGYLTLMSGFCVKPRPKALVSYWGYGDIVGPWY
                     SRPDPFYLKQPAVTREEALSTVGKSPLSEPPQKNNRGRFYLHCRQQGIWPKEVAGRDP
                     DTDDAWFNPYCPIRNVTRDYPPTMLVHGTADTDVPYEQSKQMAERLRQQGVEHQLVTV
                     SGGGHGIGNVPPAQQDDIYRKAAEFLKKRT"
     gene            9938..>11171
                     /locus_tag="VFQ79_18370"
     CDS             9938..>11171
                     /locus_tag="VFQ79_18370"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU0141694.1"
                     /translation="MDGKFHSSSRRGFLREVGGLAFAVTGHTAENGAAPWYRRAYRWG
                     QTNITEKDPERYDIEWWRKYWKRTQVQAVIINAGGIVAYYPSKFPLQHRAQFLGNRDL
                     FGELTKAAHDDGIFVMARMDSNRTGEDFFKAHPDWFARDKDGNPYRAADKFITCINSP
                     YYEEYLPGVLTEIIERSRPDGVTDNSWAGLGRSSICYCANCEREFRKKTGKAIPSGAN
                     WDDPVYRQWIMWNYERRIEIWELNNRVTQRAGGPDCIWSGMNSGSISNQARSFRDLRE
                     ISKRAHIMMLDHQRRDDDTGFQQNSDTGKRVHGLLGWDKLAPESMALYQTGPGYFRLA
                     SKPAAEARMWMIAGIAGGIQPWWHHVGAYHEDRRMYRTAEPVMRWCKDHEQCLVNRQP
                     VATVGVLWSQRNTDFYGRD"
BASE COUNT         2259 a         3288 c         3416 g         2208 t
ORIGIN      
        1 cttgcttgcg ccgttgctca agagcctgga agtcccgacg ttgtccgcgc cgaaccacca
       61 tgccccagtg taaggccact gctctcgcac gtccagaaca taagcagcat gaaatgtaac
      121 tgtattatgc gaaactcatt aaaacgcttg agatagcgat ccccttcgta ccgcgctacg
      181 cacttgtgaa attctttatg cggcgcgtgc gccatcaact gcgcaaagat cgtccggccc
      241 gagttcatgc accattgtgc cgcccaagcc gggtgggctc ggacccggaa aatcgggctt
      301 tcgaagttga aatcgttccc gggtcgaact ccagctatct cattgcaaat aggcagcttg
      361 ctggacatct ccggagagtt taccggacac tagtggtatg tactctcttc tctgctttgt
      421 acttggggct acacttcacg agcttggcga tgtcgtcttt agataacggc tcgatgacga
      481 tcttgggttc cttcagctta ggaatcttca cccgcttcag cagccggccg tcagaatcaa
      541 gagcaccgga cagatggcct tcgtttgcag cccagcggag ataagcattc actacccgga
      601 gccagcagtt gatcgatatg ggcttgacct tcccgctacc gatcaattcc atgatgcgag
      661 tcttgatatt ggctaccagc gttgctgctg gttgctcgtt cactgcgttc agtccgacag
      721 tagcgaattg ccggaatgct tcccggtacc acgctactgt cttgggggaa acatttatga
      781 tgtactgcct ctcctggacg aacctttccg tcagttccat aggcataagg tcatttcctc
      841 tttcctctgc cttggtttta cctgtaaatg attgacccgg aaggagttgg tgggcggtgc
      901 ggggctcgaa cctgcgacct cctgcttgta aggcaggcgc tctgaccaac tgagctaacc
      961 gcccaatcct gttacttctt gattctacta tgcttgcagc tatcgggcta cggcgcgcag
     1021 gcgcagggca tggttgcgaa tggcgctcgc agatgtctcc agttcatatc cttcgccgtt
     1081 tgccacaatg tgggtagcgc ttttcagggc agcgtcttca taccactggt gagtcacttc
     1141 aatagtgctt tccgtggcgg gtttcacagg aagcacggtg ttggcgatca ccttctccac
     1201 gtttgcgcca gcggtagaga ccttcagcca tgtgcgcgca ctgtccaggt tgacgccgcg
     1261 cagaacgtgg ttgaaccaca tctggttctg gttatgttcg ggatccggct tgaagtccgc
     1321 cagttgccgc catgtggcac cgccgtcttc ggacaagctg agagacagcg ctacccgatt
     1381 catcggcccg gccgcgcgag cggagacact gaagcgcaac tcttcggcgg cggcgtccgg
     1441 cccgagacgg aatgtcaggg ttcctggctg gtccggatcc tggagccggt attgcgacga
     1501 ggtggtatca aacaccagat tctccacctt ctgatcgcgc aggttctgcc ccttgtgcca
     1561 gtagctttcg tggtagtagg tacggcgggg cgcggtctgg agttgaaacg tgtttctacc
     1621 cgagaccagc cgcggcaacg ccatcggaga gacttcgacc caggtccgga atgccaggcc
     1681 ttcgagtccc accgcattgg agcccgcgcg gtccacccat tcaaacttca agtgatagga
     1741 gtaacggcct tcgacccaac gcgtgagatc gacctcgaag gggatggccc caagataacg
     1801 gttctcccac accttgttcc aggtgcggcc ggcatccgta ctgacataga tgcggttctc
     1861 gtcgcgccgg tctttacgcc agaagagtcc tttaacggcc accgcatcgg agttgtcttc
     1921 gaagttggtg aggtcgttct gctcgcccac gatcacaaac ggggtgttga cctccacgac
     1981 gagagaggct acttcacccg gccgttccgc cgagagctgc ggttttcccc gctcagccgc
     2041 taccgtgacg ttggcccgct gaggattctc tttagcgaaa gtggaagagc ggaggtccgg
     2101 aacatattcc accaacgcgt tgccgtaagc ggcgggcggt tgatcggtcc aggccagcga
     2161 gcctttccgc acgggaccca cgacccaata atccttgtaa accacgccca gatcctcagg
     2221 cagttccgtg agcaggtaac ggtttctcgg ccggtagtaa aactcgatgg attcattcgt
     2281 gcgcaacgcc aggttcatcg tgtgcgcatt cgagtaggga tgatgattcc tgctgggctt
     2341 tgccccacga aaaaccgatg ccatgtccgc cttttcatca aacgggtaat agggatcagg
     2401 aggattcttc tgcttcagaa acaggtccgg ggcagacacg agttcgtcga cgctggcgat
     2461 acggcggccg tcttcgagaa acacgtagcc gaacatgtcg gtatcgatgt agttccattt
     2521 gccctcgaac caaatttccg gaacggagtg tcccggcatg gatcgcgtgc gggcccgcat
     2581 gcccgcctcg ttatacagtg ccgtcatggc cttgctgttg ttgccacata acgagaagcc
     2641 gtaaaccgcc atcaacttga gcgggtcgtc gacataatgg ccgcgctctt cgatgggccc
     2701 gttgaagtga atctggtggg ccatgccgaa cttgtgaagc gcgagaacct tttcacgctc
     2761 gctgccggcg cctttcgtga cggacgagac aatatcggcg acgtcccagc agtttggctg
     2821 cgcctgtccg atcagccgaa tggagaactg ctcggcggaa gccgccagcg caaagagaag
     2881 aacgagtatc gtaggccgca ttgcaatcca gttattgaag gtccagagat ttcaggatct
     2941 cccggatggc gaagtcccgc aagtgctcca cgtcatcatt ccgggtggtg acaaaaaact
     3001 cggcgtccgt gtagatgcgg ttgtcgagga cgatgccctg aaacatggct tcctggcggc
     3061 ggtggatttc cgcgcgcaac gcttcccatc gcgaggggtc tttccgcttc accagatttt
     3121 ccagggtctt gagatagcgg acatcgtcga tgccttcgcg ggcagcctcg aaggctaaag
     3181 tgcgcaccgg ttcagcgccg tccacggaag gatagacggc tacccagtcc gcgtcggatt
     3241 gaggcaaagg atcgttcgct tcaccgcgac cgtcgaaatc gttccaggga ttgttgatgt
     3301 agtagttcca tgaccagaaa aacatcgcct cgcttccgta gaaagcgggg atttggccgt
     3361 acaggaggcg gcatttcccg aaggactgat ccgcggtgat gttgttgtag gtccagaagc
     3421 gcttgccgaa ttcacgcgcg aggcggctca tatgttccag gtcgcgattg gcgaccacga
     3481 tatcggaact gtgcagcagc gggccggggt ccttccgcgg ctcgaagaac acaccgtaga
     3541 ccgggacctc cgggaagtgc ttcttgagca gatggtagcg gtacgggtga taagccatga
     3601 tgaactttgt cggctcgtcg ttgatcagca gcgcaagggg aggccagccg gcttctttgg
     3661 cgtgtgcaaa gatctggcgc aggccttcga tgtagggctt ttcccaacgc ggatcggtca
     3721 tgtccatgag ggtgacctcg cggcctccga ggtcgcgctt catcaggcgc aggtcgaagg
     3781 cccgggcaag cgcgatttcg tacatgccaa gccaactgtt gccgagcgac ggaacaatgg
     3841 ggccgcgcat tccggcctgc tgcatgccgc ggacgaaggc gtcgaactcc gtgaaatcca
     3901 gccgaacctt accggcgtca ttttggacgc ggatgggatg cgagccccag aaccattgca
     3961 gcgcatccat gccgtagcgc ttcatcatgg cccattcccc cttttcgaac ttccggccgc
     4021 tgagaaacgc gccgaaacgc acgccggggt cgtccagctt gaacggcagc acgcggactt
     4081 caagcggcac ctcgagcgcc gcgccgccgg gttgcgccat gcggagtgtg gtctggtaca
     4141 ggccaggcgc ggcgtcgggc ggcaccgacg cggtaaccca ataaaggcgg ctgctgccgg
     4201 cggggacagc ctgctccgta accgggcgga ggaaaaccgg atagcggacg aagcggccga
     4261 tacgcttgat tcgtgtggta tcgaattcac cggagctgcc acccatcgat ttggtgcgga
     4321 gttggacggg atgaaacaga acatcctcga tcttcagcca cgatggtccc cgcagttcgg
     4381 gacggatggc cgggatttcc cgcaaagcgt aaagggagaa aggccgcgat tccatctcgc
     4441 cgggagtagc ggtgatccgt agtctggtga gcgcttcgcc gggagcgggc cgcgaattgg
     4501 gcagcaattc gaaattgcga tcccgctgat agagcagcca gccgcgttgg tgatcggctt
     4561 ccgaaggctg aagcatctcg cccgaaaccg gcagaaactc ctcctggatg ccggcaggaa
     4621 cgggcggaac gggaaggtcg cccggttgtg cgaacgccgc ggcacacacc aaaaacgcaa
     4681 aggggcactt gagagccatt tctccgatga taagccgaac cagcgggtac agacggcggc
     4741 caacaggtta tgctttctgg taaggcaaat tcctatgtgg atgaacaatc gtttcccgat
     4801 gctgttcctc gtcatggcag tgctgggcgt ttacgctgcc gctccgccgg tttcgaatcg
     4861 agcgccggca cccaacgata tcggttatct gccggctgac ggagccaaag tgggcacgaa
     4921 tcctcccgcg ctggcctggc tgcccgaacc gcacgcggat gcttacgccg tccaactcgc
     4981 gcgcgacgag ggattccaga aaggcgtaat cacgatccgg cgcacaccgt atgtgctgta
     5041 cacgcatacc gcgacgctcc gtcccggaac ctggtggtgg cggtacgcat gtctcgatgc
     5101 gcgggggaac cagtcggcgt ggagccaggc acggcacttc accatcgccg gagacgcgca
     5161 gccgttccca cgtccatccg acgaactggt gcgcgagaga attccaaaaa gccatcctcg
     5221 cctgatgttg cggccggagg aggttgagat gttccggcag gcgcgccttg gaccggaaaa
     5281 ggcccgttgg gaagaactcg tcaaggcagc cgaggaatac ctgaaggcgc cgcttatgcc
     5341 cgaaccgccg ccctggaccg atggaaaatg gaacgctccg gagtggaggc gcaacttcca
     5401 ggacacgctc cgcgcagtgg agaaggccga gactcttgct ttttgctact tgctgtccgg
     5461 cgacaaacgc tatggcgaag gagcacgcaa atggctgctc catttcgcct cctggaatcc
     5521 ggccggcagc accagcatgg acgtcaatga cgaagccgga atgccgatcc tctatctcac
     5581 ttcgcgcgct tatgattggg cctacgatgc gttgagcaaa gaagaccgcg aagccatgcg
     5641 caagatggtg cggttccgcg gagaggaagc gtacgactgg ctgcacgaca agccgttcga
     5701 gcagcaggcc tataacagcc atggcggccg gatgtggcac ttcctcggtg aggccgcgat
     5761 cgcttactac ggtgaggtgc ccgaagccac caaatggctg gactacgcgc tgtcgatatt
     5821 ctggggatgg tatccatcct atggcgatga agacggcggg tgggcgcagg gttattccta
     5881 ctgggcgagc tatgtcaacc gctctacgtg gtggttcgac gcgctgcgag ccggtctgct
     5941 catcgacggt acggagaagc ctttttaccg gaatgtcggg ctcttcccga tgtacgtggc
     6001 gccgccgaat ggatcgctca cgggattcgg agatttcgcg gaagccgaac cgaacgccag
     6061 agcggccgtg ccggtgtcgt attttgcacg gatgcgcgga gtaccggaat ggcagtggtt
     6121 cgcggaagct tggggcaagg gggaaggaga gaccggtccg atcggtttca tccgctccgc
     6181 gcgcccggac ccgccgaaag tgacggcacg tccgccgtcg aactggccgc aagccaagtg
     6241 gttccgcggc gccgggtggg tctcgctcca ttcgggcatg atgaacccag ccgaaaacgt
     6301 gcaggtcatg atgcgcgcag cgccactcgg gaatatcagc cacagccatg ccgaccagaa
     6361 cgccatcgtt ctcgccgcct tcggctcgcc cctgttggtg aacaccggta tccggccgtg
     6421 gtatggcagc ccgttcagca aggaatggta ttggacaacc aaggcccaca acgccctgga
     6481 aattgacggc cagggccagc ccaagacggc ggaagccacg ggggaaatca tcgtcttcca
     6541 acccggcaaa gagtacgact acgttgtggg cgatgcgacg cctggttacg gtgcgaacat
     6601 ggaacgctac cggcggcacc tgttgttcct gaagcccgac gtcattgtgt tggtggatga
     6661 ggtttcggcg cgcaaaccgg tctcgctgaa gttctggctg cacgggcggg caccatttga
     6721 actgaacccg gcaggcgacc atgttgcact tactttcggc aaagcatcgc tgtccggttt
     6781 cctgcgttcg ccgggcggcg tgcaaacggt tcaaaccgac aagtaccctc tcccgccgga
     6841 acaaggtcag acccggccgg agtggcacct ctcgtccgag acgaaagcga agcataccaa
     6901 cgcgcgtttc gtcgcggtac tcggagtggg caaagctggt gagaagatcc cgctcgactc
     6961 cgtagaagac gcgagcagcg gcggccgtat tgccgttcaa ttccgccgcg ccgggaagcc
     7021 ggtaagcatc tctatcgatc cggccgggcc aacggtatcg atacgataat gtcgtgagca
     7081 gtcgtgtaaa gccctttctc agcctgtgga tcgcctgtac tgtgctgccg gcccaaacct
     7141 ggattgcggc cacagacgag gccctggtta aacgcacagg aacgtggacg gcagcggaat
     7201 tccggcgcgc cagttccggg cacctgctga cggtggaaga tggggcggca ttggagctca
     7261 ctttcaccgg tacacgcgtg gtgttgtcac tcgatgccca tggcctttcc tatgcgcatc
     7321 ttggtctcga aaatcttggc attctggagg tcacgctaga cggccagcca agcacggcgg
     7381 ttctgccgca acaggaggac cgcgatgtcg tgctcgctcg cgaacttcgc aatggcgcac
     7441 atacgctgcg cctggtgcat cgcgcaactt ccgcgggaag tggcgcgcgc gttgccggat
     7501 ttcgcattct gagcggcaac gaaggcggcc tttcctttct cctgcacggc gaggccaacc
     7561 gctatttgaa cgacattcgc gcagtcgtct cgaagaacgg ccaggtggtc cgcaacgggc
     7621 tgtttcgcaa ttggctcacc ggacaatgcc gcattgccgg actgcccgcc ggaccaggat
     7681 atgaagtaga gttgatggcc gccggctggc aaactcagca cctccggaat atcgagatca
     7741 aggccgggca ggaaacggaa ctgcggcccg tgtaccttct ccgtaagccg gaaagcacga
     7801 cgagcggtgt tgaattccct cgcgccggcc aacccgcaat cgtacggtcc ggagaatcgt
     7861 tcgctacgcg cgtattcctg gccggagcat ccatcgagtc cgttgaactg cgccggcaaa
     7921 tcggaccggc aaccatttcc cgcgaggtcg tgtttcgcga agacaaagcg cgtgagcagg
     7981 acggcagagc ggaagggatc atcacattgc ccaccggcgt tgcacccggg ctttacgacc
     8041 tggttttccg gctatcgaag aaggccagcc ggagtgcgcc gcgcgccgtc tacgtcgttg
     8101 cggaataccc caagaacccc gtcttcgtta cgttcggcca catggatacc tttgggcagg
     8161 aggctgccga gtatctacag cgtctggccg gtctctccaa tctgattggc gcggacatgg
     8221 tgctggtcag caatgaggtg aacgccgcct acgcgttggg cgcgttcgcg cgactggaca
     8281 tgccgcacct catcacattc ggtaatcatg aggtggccgg ccacgaagaa ttttatggaa
     8341 acgccatcag catgatcgat tacgggccgg aactcagcat cctcaatttt tctcatgcct
     8401 ggcatggcga cctttcacat gcgtacgctc tgctcgactc gcgagcgaaa acccgttgca
     8461 aaatcattaa cgccttcgag cacgatgcgc cggtggaggc gatgctcgac cggtacggaa
     8521 tctctttcct tcatgaggcc cacggacccg atccaaaggt gatgcagatc ggccgcactc
     8581 ccacgcaacg cgccggcaag gtgaacagcg agagtttccg ggtggtgcgt ttcgaaggct
     8641 gccgcccggt gtccttcacc tacgcgggac atccttcggc gccgatcccg cttccgcgcc
     8701 accagcccgc gccaatgcag ctttcttttt cggcacccaa tgacggcacc gcgcgaaggg
     8761 tgaccgcgcg tatttccaac cgttgggcac agtctttccc ccacgggcga gtggttttcg
     8821 tgatgccggc gggcgattgt accgtggagg gcggccgtat ggaatccgcg atcgccagcg
     8881 acgacgggcg cttcgtcgtc ctgaccgcgc gcgtcgatat tcccgcgaat gccgatatcc
     8941 aggtgactgc cgaatcgcgg cggcgatgac accgtctatg tggtaggctc gggccatgca
     9001 gcgcaggagt tttctggttt caacggcagc ggcaatgctg aatgccgctc cggaacaacc
     9061 gcgcacgttt gtctataaaa ctgtgcccgg atgtgacatc aaagcagacg tttactatgg
     9121 cggaaaggct gccgccgtgt ggatccatgg cggtgcgttg atctcagggt caagacgtct
     9181 gtctcgggat tcgcgcgttt tgcggtcgct cctcgaagcg ggcttcaccg tcgtatcgat
     9241 tgattaccgt ttagcgcccg aaaccaagct gcccgaaatc atcgaagaca tccgggacgc
     9301 gttccgctgg cttcgcaccg aggccggctc gctgggtatt gacggtgacc gtgtcgttgt
     9361 atgcggcggc tcggcgggcg ggtatctgac cctgatgagc gggttctgcg tgaagccgcg
     9421 gccgaaagcg ctggtctcgt attggggcta cggcgacatc gtggggccat ggtattcccg
     9481 ccccgacccg ttttatttaa agcagcccgc agtcacacgc gaggaggcac tgagcacggt
     9541 aggcaaatcg ccgctttcgg agccaccgca gaagaacaac cggggccgct tctacctgca
     9601 ctgccgccag caaggcatct ggccgaagga agtggctgga cgtgacccgg atacggacga
     9661 tgcctggttc aatccctact gccccatccg caacgtcaca cgggactatc cgcctacaat
     9721 gttggtgcac ggaacggcgg acaccgatgt gccctacgag caatcgaaac agatggcgga
     9781 acgcctgcgg caacaaggcg tcgagcatca gttggtcacc gtgtccggcg gcgggcacgg
     9841 aatcggcaat gtcccacccg cccagcagga cgacatctat cgcaaggccg cagagttctt
     9901 gaagaagcga acctgacggc ccggggagca gatcagtttg gacggaaaat ttcattcgag
     9961 cagccgccgc gggtttcttc gcgaggttgg cggtctcgca ttcgctgtaa cgggccatac
    10021 ggccgaaaac ggcgctgcgc cgtggtaccg gcgtgcctat cgatggggcc agacgaacat
    10081 cacggagaaa gatccggaac gctacgacat cgaatggtgg cggaaatatt ggaagcgaac
    10141 acaggtgcag gcggtaatta tcaatgcagg cggcattgtt gcttactacc cgagcaagtt
    10201 tccacttcag caccgcgccc aatttcttgg gaaccgcgac ctgttcggcg aactgacgaa
    10261 agcggcgcac gatgacggaa tcttcgtcat ggcccgtatg gattcgaatc gcaccgggga
    10321 ggatttcttc aaggctcacc ccgactggtt tgcccgcgac aaagacggaa atccctaccg
    10381 cgccgcggac aaattcatta cctgtatcaa cagcccctac tacgaggagt accttccagg
    10441 cgtcctcacc gagatcatcg agcgctcgcg cccggacggt gtcacggata acagttgggc
    10501 gggcctgggc cgcagcagta tctgctattg cgcgaactgc gagcgggagt tccggaagaa
    10561 gaccggcaaa gcgattccct ccggcgcgaa ttgggacgat ccggtatacc ggcagtggat
    10621 tatgtggaac tatgaacgcc ggatcgagat ctgggagttg aataaccgcg tgacacagcg
    10681 ggcgggtggt ccggactgta tctggtcggg catgaatagc ggctcgatat ccaaccaggc
    10741 gcgatcgttc cgcgatctgc gcgagatctc caaacgcgct cacatcatga tgctcgatca
    10801 ccagcggcgc gacgacgata cgggttttca gcagaacagc gataccggga aacgcgttca
    10861 cggcctgctc ggctgggaca agctggcgcc cgaaagcatg gccctgtatc aaacgggacc
    10921 aggctatttt cgtttggcga gcaaacccgc cgccgaagcg cgaatgtgga tgattgccgg
    10981 aatcgcgggc ggcatccaac cctggtggca ccatgtcggg gcttaccatg aggaccggcg
    11041 catgtatcgc acggccgagc cggtgatgcg ctggtgcaag gatcacgaac aatgtttggt
    11101 aaaccgccag cccgtggcca cggttggagt gttatggtcg cagcgcaaca cggatttcta
    11161 tggccgcgac c
//
LOCUS       DASTMK010000428         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_373679, whole genome shotgun sequence.
ACCESSION   DASTMK010000428 DASTMK010000000
VERSION     DASTMK010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373679"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1923)
                     /locus_tag="VFQ79_18375"
     CDS             complement(262..1923)
                     /locus_tag="VFQ79_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0141695.1"
                     /translation="MAQFLLDMEGHVVTWSPGASRIYLYSEDQAVGKHVSALYSIDEN
                     NGSEPQRELTRSAAEGHFGNEGWHARKDGTRFWANVITLALRDARGELQGFARIVRDF
                     SERHERDEKLRHSRARVRRAPLRSTIAGIVSGEFDRVPEANDAFLKIVGYSREDLLNG
                     SLRWRDLTPPEYAALDDLAHEEALRFGACTPYEKELLSKDGARVPVLIATAVLKLSPF
                     RWITFVQDLRDRNRNENIGDEDVESEQDFEEMVGNSAALRRVLRNVEVVAPTDANVLI
                     VGETGTGKELVARAIHRLSPRRKQPFISLNCAAIPTGLLESELFGYEKGAFTGALGQK
                     LGRFEMAHRGTLFLDEVGDIPLDLQPKLLRALQEKSFERLGGTRTIPIDVRLIAATNR
                     NLTQMMGDKLFRSDLYYRLKVFPITTPPLRDHPEDIPILARHFTQKYAQQMNKEIERI
                     PSETMRALVNYAWPGNVRELENFIERAVILSRGPGLRAPLSELQSSASQAAGDSTLEH
                     VEREHVIRVLRETGGVVSRAAARLGMPRTTLNALMRKLGISRKDL"
     gene            complement(2236..2676)
                     /locus_tag="VFQ79_18380"
     CDS             complement(2236..2676)
                     /locus_tag="VFQ79_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141696.1"
                     /translation="MITSGVLLIQPDALRPRCFQVEADPHLNGWTVVTHGLTSQQLEK
                     ELSATGWTFFFMAGPLRMTAFGFDREKTVYRALKRGIRAVRQQGCNCLQIESVEMHSF
                     LGIPRVSMSIRPRHIQRGMLFAGPSLADGASITAHGLRSNVAAT"
     gene            complement(2764..>4522)
                     /locus_tag="VFQ79_18385"
     CDS             complement(2764..>4522)
                     /locus_tag="VFQ79_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0141697.1"
                     /translation="RERTKVTQTKLTGAFLIVSGILSAQVAPPQDENASRMAGGDAPI
                     YRVTVTARTARAINYQHRSGATKIDFRGTELLAGARGEAKVESKQGYIEIEVEFDNLR
                     PANKQGAEYLTYVLWAITPEGRTANLGEILLNGTKSKLNVTTELQVFALVVTAEPYYS
                     VTRPSDLIVMENVVRPDTKGKVEEIEAKYELLQRGQYQRLANPLALKLDQKTPLELYE
                     ARNAVQIARAVGADRFATETFKKAEQSLAQAEAYQTSKAGRKPVTMAARETVQTAEDS
                     RAIAVKRQEDEALAAERQRSADREARLETERVAAKAETDRISREAETAQAKAQAESER
                     LTREKNEQAAAAAAEAERVRLETQARMAAAASEADRLRVENEARLATAAADADRARLE
                     NEARATAAAAEADRLRRENEAQKAANQAELDRAAKDRAQAEAEKAALRAQLLSQFNAI
                     LQTRDTARGLIVNMSDVLFDTGKYTLRPLAREKLAKVAGIVSGHPGLKLDVEGHTDSV
                     GSDDYNQRLSEHRGAAVRDYLTQQGMSSGSVTSKGLGETQPVASNDSASGRQQNRRVE
                     LVISGEVIGTAIGTPLAAR"
BASE COUNT          773 a         1406 c         1323 g         1020 t
ORIGIN      
        1 catatcgtta tggccgaggc ggtcggacac ggctggtggg acgggtacgg gaaggcggct
       61 cgcagcggat gccgcgcgcc aagttggcga gaaccgccca agttgagatc tggcctgcca
      121 gcggatggtc cacgtcactt gctcggtatt taccgccggt tagcgcaacg gaaagtcgga
      181 tctataataa tcgggcagct gtgcgcccgg acctgacatg cggtcaggcg accggcagcg
      241 gtcagcgatc cgtcgacgca gtcacaggtc ttttcgtgaa attccgagct tccgcataag
      301 ggcgtttaga gtcgtccgag gcattccgag acgggctgcg gcccggctga cgactcctcc
      361 ggtctcccgg agcacgcgta tgacatgctc cctctcgacg tgctccagtg tgctatcccc
      421 cgccgcttgc gacgcactgg actgcaattc cgacaaaggg gcgcggaggc cgggacctct
      481 tgaaagaatg acagcccttt caatgaagtt ctcgagctcc cgaacgttgc ctggccacgc
      541 ataattcacg agcgcccgca tcgtctccga cggaatcctc tcgatctcct tgttcatttg
      601 ctgggcgtac ttctgggtga agtgccgtgc cagtattgga atgtcctcag ggtgatcgcg
      661 caacggcggt gtcgtgatcg ggaacacctt gaggcggtag tagagatcgc ttcgaaagag
      721 cttgtcgccc atcatctgag tcaggttgcg gttggtggcg gcgatcagcc ggacatcaat
      781 gggaatcgtg cgggtccccc ccagccgttc gaacgacttc tcctggaggg cacgcagcag
      841 tttgggctgc aggtcaagcg gaatatcgcc aacttcgtcc agaaacaggg tgcccctgtg
      901 cgccatctcg aagcggccca gtttctgtcc caaggcgccg gtgaaggctc ctttttcgta
      961 accgaacaac tcgctttcga gaagcccggt ggggatcgca gcgcagttca aactaatgaa
     1021 aggctgcttc ctccgggggc tgagccgatg gatcgcgcgc gcgaccagtt ccttcccggt
     1081 gccggtctct ccgacgatca gcacgttcgc gtcggtcggg gccacaacct cgacgttgcg
     1141 gaggactctc cttagagcgg cactgttgcc gaccatttcc tcaaaatcct gttcgctctc
     1201 gacgtcttcg tccccgatat tttcattccg gttgcgatcg cgaaggtcct ggacgaacgt
     1261 aatccatcgg aagggagaga gttttaatac cgcggtggcg atcaaaaccg gcacgcgggc
     1321 gccgtccttc gaaagcagtt ccttttcgta aggcgtgcag gccccgaacc gcagtgcttc
     1381 ttcgtgtgcg agatcgtcga gcgcggcgta ttccggcggc gtcagatccc gccatcgcaa
     1441 gctgccgtta agcagatctt cgcggctgta cccgacaatt ttcaagaagg cgtcgttggc
     1501 ttcgggaacc cgatcgaact caccggatac gatcccggcg atggtagacc gcaaaggcgc
     1561 ccgtcgaacc cgtgcgcggc tgtgacgcag tttttcgtcg cgctcatgac gctcgctgaa
     1621 atcgcggaca atccgggcaa atccttgcag ttctccacgc gcatcccgta gcgccagcgt
     1681 aataacgtta gcccagaatc gcgtcccgtc cttcctcgca tgccagcctt cgtttccgaa
     1741 atggccttcg gccgctgacc gggtcagctc gcgttgcggc tcagaaccgt tattctcgtc
     1801 gatggaatag agagcggaga cgtgtttgcc aacagcctga tcttcgctgt agaggtagat
     1861 acgcgacgcg cccggggacc aggtgacgac atggccttcc atatccaaca ggaactgtgc
     1921 caagtcctcg tccgaagcgg cagccttcga cgccgtggcc gaactatcaa attctgttga
     1981 aggtacctta tgacagacaa ttgtccgaag gagcgcctcg agctgtgggg ggccgtattt
     2041 cagcacccaa ctggccgacc gtgaccgggg gacttccgag gccaacgctc ctcctcggcc
     2101 ctgtctcggc accaaccagc tttctgaggc gtccagtgtt ggccctgtat tgcaggcagg
     2161 tcaacgccga accgggccac cgaatccatg ctagcacctg acggcgcttc gagtggcccg
     2221 attgaccata tttcgtcagg tggcggctac gttgctgcgc agtccgtgcg ctgtaatgct
     2281 cgctccatcc gcaagcgacg ggccggcaaa aagcattcct ctctggatgt ggcgcgggcg
     2341 gattgacatg cttacgcgtg gaatccccag gaaggaatgc atctccacgc tttcgatctg
     2401 aaggcaattg catccctgct gtctcacggc ccggatgccg cgcttgagcg ccctgtagac
     2461 cgttttttcc cgatcgaatc cgaaggccgt catccgtaga ggcccggcca tgaagaagaa
     2521 cgtccagccc gtggctgaga gttccttttc gagctgctga gaagtcagac catgcgttac
     2581 aaccgtccag ccattcagat gtggatcagc ttcgacctga aagcatcgcg ggcgcaacgc
     2641 atctggttga atgagaagga cccctgacgt gatcattgga tttctccgtt gttcatacgc
     2701 cgcgaacagc cttggggccg gcagcagttg cctgtcggcc ccttggatcg cggtagcccc
     2761 cgcctacctg gccgcgagtg gagtcccgat cgccgtgccg atgacttcgc cggagatcac
     2821 tagctctacc cgtcgattct gctgccggcc gctcgcgcta tcgttcgatg ccaccggctg
     2881 agtctccccc agacctttcg acgtgaccga accagatgac attccctgct gcgtcaggta
     2941 atcccggacg gccgcgcctc gatgttcgga aagccgctga ttgtagtcgt cgctacccac
     3001 gctgtccgtg tgcccttcaa cgtccaactt caggcccggg tgtcctgaaa cgataccagc
     3061 caccttggca agcttttcgc gcgccaatgg tcgcaacgtg tacttacccg tatcgaatag
     3121 aacgtcggac atgttgacga tcaggccgcg cgctgtatca cgggtctgga gaatggcatt
     3181 gaattgactt agtagctgag ctcgcagtgc cgccttttcc gcctcagcct gggctcggtc
     3241 ttttgcggcg cggtcgagtt cggcctggtt tgccgccttt tgcgcttcgt tttctcttct
     3301 gagccggtcc gcctcggcgg cagcggcagt ggcccgcgcc tcgttctcca gccttgcgcg
     3361 gtccgcgtcg gccgcggcgg tggcaagacg cgcttcgttt tctaccctta accgatctgc
     3421 ttccgacgcg gcggcagcca tacgggcctg ggtttccagc cttacgcgtt ctgcttcagc
     3481 cgcggctgcg gccgcctgct cattcttttc gcgtgtcaag cgctccgatt cggcctgggc
     3541 tttggcctgt gctgtttccg cttcgcggct gatcctatcc gtctccgcct ttgcagcaac
     3601 tcgctcggtc tccaggcgtg cctcgcgatc ggcggaccgc tgccgttcgg ccgccagggc
     3661 ttcatcctcc tgccgtttga cggcgatcgc gcgggagtct tccgcggtct gcaccgtctc
     3721 gcgcgccgcc atcgttaccg gttttcgccc agccttgctg gtttggtacg cttcggcttg
     3781 ggctagactc tgctcggctt tcttaaaggt ctcggtggca aaacgatccg cgccaaccgc
     3841 gcgtgcgatt tgaacggcgt tgcgtgcttc gtagagttcc aaaggcgttt tctgatccag
     3901 ctttaaggcc aggggattcg cgaggcgctg gtactggcca cgttgcagca gctcatactt
     3961 ggcttcgatc tcttccactt ttcccttagt gtccgggcgc accacgtttt ccatgacaat
     4021 cagatcgctg ggacgggtca ccgagtagta cggctctgcc gtgacgacca aggcaaagac
     4081 ctgtagttcg gtggtcacat tgagtttgct ctttgtccca ttcaagagaa tctcgcccag
     4141 gttggccgtt cggccctccg gcgtgatcgc ccacaggaca taggtcaggt attcagcacc
     4201 ctgtttgttt gccggccgca gattgtcaaa ttcgacctcg atctcgatat agccctgctt
     4261 gctttctact ttggcctccc cgcgagcgcc cgccagtagt tccgtgccgc gaaagtcgat
     4321 ctttgttgcg ccgctgcggt gctgatagtt gattgccctt gcggtccgcg cggttacggt
     4381 gacgcgatag atgggcgcat ctcctccggc cattctggag gcgttctcgt cctggggtgg
     4441 cgccacctga gcggacaaga tgccggatac gatcagaaat gcgccggtaa gcttcgtttg
     4501 agtcactttt gttctttctc gc
//
LOCUS       DASTMK010000429        12886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_278373, whole genome shotgun sequence.
ACCESSION   DASTMK010000429 DASTMK010000000
VERSION     DASTMK010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278373"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(636..3272)
                     /locus_tag="VFQ79_18390"
     CDS             complement(636..3272)
                     /locus_tag="VFQ79_18390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141698.1"
                     /translation="MNWWSRLVGRKQMEEQLEKELSFHLDQHAGDLIASGCTPEEARR
                     QARLSLGGFEQVKEKCRDARGTRWLEDLVRDTRHTFRTFRQKPGFGAVAVMILGLGVA
                     ATTVMFAVVHSVLLKALPFSGSDRLVTVNGYTERLGDFWGFSNPDFDDLRRSSRSLTV
                     AAWTYGGGTISEPGEPEYANGRKISAELLPTLGTLPLLGRAFQPDEDRPGAPPVTIIS
                     YALWQRRFSGDPAAVGRTLVFEGKTYTVVGIAPASFQLSGDADVFTPLGQSTDPRMQN
                     RAARFIHLVARLRQKVTLSEAQSEIAVIANQLAKEYPKSNEGITMRVHPLQQELVGDV
                     RETLWLLLSGVGLLLLLACVNIASLFLTRAISREREFAMRVALGAGRGRLMRECLTES
                     VAFGICGGLLGIVLAAVSLRPFVVFWPGSLPRAEEIQLDWSVLWFAIGVSLLSGLLFG
                     VVPALRVPSHSLGHALRAGGRALAGGSRRLHSAFVVSEIALACVLLVCAGMLGKTLLT
                     LSSLDPGLNMHNVLTARFALSPSAVANPSQIRSAWQDVLDRARRVPGVEFAALTDIVP
                     MREGENSVTYRTTAAPLPANREPFALASSVTPDYLKVMGIPLREGRFFNEHDREGSES
                     VVVIDENLARNAFGEQHAVGRRLWIPAFGDAPVQVVGVVGHVRHWGLARDDQSRVRDQ
                     IYYPFAQVPVPLLRFFSSIMSITVRTKIPPMDVIKPLEFELRGAARDQALYELRTMEQ
                     LASASLARHRFLLLLFGVFAGIALILAATGIYGVLAYLTGQRVPEIGVRMALGATVRD
                     VMALVLRQCLQMAFAGIGLGILAALAAGRVLQRLVQGMQPVQVATLTAMITILLAAAV
                     VAGFIPARRASRVDPVRALRQE"
     gene            complement(3269..3616)
                     /locus_tag="VFQ79_18395"
     CDS             complement(3269..3616)
                     /locus_tag="VFQ79_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141699.1"
                     /translation="MTTPKSDLPQGTLDLLILKAVAAGPLHGYAIAQRLEQVSRGVVQ
                     VPEGSLYPALHRLENRGLLTADWKKTETGRDAKFYRLTRKGHTQLEAETASWQRLTEA
                     VGFILAMADGRAK"
     gene            complement(3845..4159)
                     /locus_tag="VFQ79_18400"
     CDS             complement(3845..4159)
                     /locus_tag="VFQ79_18400"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0141700.1"
                     /translation="MTKKLTARALARLGASRDVWQEVAEGVAAIKAGRGKRTSVQPRS
                     PIVRARLKSELTQEQFAALLGVSRRTLEQWEQGRRVPSGAAKTLIRVAERHPEVLREM
                     AA"
     gene            complement(4156..4467)
                     /locus_tag="VFQ79_18405"
     CDS             complement(4156..4467)
                     /locus_tag="VFQ79_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141701.1"
                     /translation="MFEFIESPAFERFRAYYLDDDEFGELQQFLMRHPEAGKVVPGSG
                     GMRKLRWRLGGIGKRGGLRVIYYVRYEPNQFWMLTLYAKAKTENVPAHILKQLQGDFR
                     A"
     gene            4788..5381
                     /locus_tag="VFQ79_18410"
     CDS             4788..5381
                     /locus_tag="VFQ79_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU0141702.1"
                     /translation="MPKAAMEESREITGLLHAWRSGDATALDKIIPCVYAELHRLAHS
                     YLSREQSRDVLQTTALVHEAYLRLVRASEIDWKSRIHFFAISATLMRRILVEFARTRR
                     AVKRGGNEHRDVFDEDVPMAVPVQEDLVAIDEALTALSEIDPREARVVELRFFSGMTV
                     EEISAELDISPDTVLRDWMHAKVWLLRKLSLETRRGG"
     gene            5371..7818
                     /locus_tag="VFQ79_18415"
     CDS             5371..7818
                     /locus_tag="VFQ79_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0141703.1"
                     /translation="MEDERWNRSKDLYAATLRIEPGQRRAFLAHACGTDQSLRRELNT
                     LLDYESRARNFLELPAVDVRAKFRSGPTDAPDIVGTSLGHYQVLEKAGTGGMGVVYRA
                     YDRQLERDVAIKLLAGEPETTSRMRLLDEARAAARLAHPGICAVYGVTSANGHACIVM
                     EFVPGEPLADLLKNGPLGTDEVIRFGGQLAEALAHAHERGVIHGDIKSANILVTPERR
                     IKILDFGLARRMRPATSPTPGPAFPMGALAGTVAYMAPELLLGREPDVHSDIWSLGVV
                     LYEMAAGFRPFSGPGTLEVCSTILNSPHQPLPGRVPEPLRSVIERCLAKEPERRFQHA
                     LELGSELHTVPWRAAVTRALSRKIAVLPFENLPVGGATGHLAQGIHDAVITDLYRLSG
                     LRVIARHSSMALGKEHLSRVPARLNGVDGVMTGTISIAGDTIELSIQLADATSGEIVW
                     ANRYNSLLADTARVRKDIVARVCEAMRLQCDAQQAQAGITRVKPETSALYMKARFFLN
                     KLTPDGLAKGLAYLRTAVEADPGEPLVHAGLALAYVLIAHQASVRLVGDPFELAKPYA
                     AKAIHLNPQLPEAHQALAEIRTYLDWDFAAAEHEYRTALALSNDNLAEAHAHYSWYLM
                     LAGRESEQMAHMKMAQELDPLNPVYHVWMGQHYAIAGRWTDVTPEAQAALEINPGTPD
                     ALVLLGHAYLQRGMIAEGIECQEQAAAIDLRLSTYALAATYARIGRADDARRIAAELR
                     KNPDHWNAYGLASIYAALNDADEALFWLERMYASRHMYTPWVRIAPEWASLRDDARFQ
                     KLAHRLPVPIGVTWPDQ"
     gene            complement(7870..9279)
                     /locus_tag="VFQ79_18420"
     CDS             complement(7870..9279)
                     /locus_tag="VFQ79_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141704.1"
                     /translation="MIARLLLTGVVSLAALVAEPLRLHPENPHYFLFRGKPAVLITSG
                     EHYGAVLNLDFNYTRYLETLNRDRLNLTRTFTGVYSEVPGSFQIASNTLAPKPSRFIS
                     PWQRAGDKFDLTKWDQAYFARLKDFLRKANDHDVVVELVLFCPYYKDEMWEVSPLNAR
                     NNINDLGNVGREEVLTLRHPGLVKVQEAMVKKIVTELNGFDNLYYEICNEPYFGGVTL
                     EWQAHIAGVIAETEKTLPKRHLIAQNIANGSRKIENPNPLVSIFNFHYSRPPESVAMN
                     YSLNKAIGNNETGFDGVEDDTYRIQGWEFLLAGGALYNNLDYSFAAGSEDGTFQYPSA
                     QPGGGSTRLRRYLRNLHDFMDRIDFIHMKPDRSILAGGLPENASFQALVEPGKSYAVY
                     IHHGRVVKDAKPRYQVDATPHQLALELELPAGTYHMMWVNPKSAKVEKSSTLRHPGGK
                     TTLDSPEYSEDIALRLTAN"
     gene            complement(9276..10529)
                     /locus_tag="VFQ79_18425"
     CDS             complement(9276..10529)
                     /locus_tag="VFQ79_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEU0141705.1"
                     /translation="MDLQDQLQQLRRRIARIDRKYAGATARPQSPAPERRLPERYSIE
                     HWLSGQEVETPHGRHFETERVWERHRRHGSIGISDLNDLPPDLFDELTGRAVDPAAPH
                     HWAFLDTETTGLAGGSGTYAFLIGVGRITAEGFHLRQFFMRDYAEEASLLFALERYLS
                     EFSVLVTYNGRAYDQPLLETRYRMSRRRSPFDHMGHVDLLYGARRLWKLQFESCRLVD
                     LENQVLGVEREGDLPGEMIPYVYFEYLRRREAFRVAPIFHHNAIDILTLACLCAIVPW
                     AFRSPAEARLRRGPEMVGIGRWLRKCGRMEDALLLFRRAVDAGLPDDLLFRTLWEVAQ
                     LERKLGRMDAAIAVLTELSACRNPFRVAALEDLAKYYEHRERNYTMALEMTRAALACE
                     DSNPLRHREQRLARRLAVPRTRRLL"
     gene            complement(10564..12486)
                     /locus_tag="VFQ79_18430"
     CDS             complement(10564..12486)
                     /locus_tag="VFQ79_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU0141706.1"
                     /translation="MIQLSAAGKRFGPKTLFEGLDWLIGNRDRVGLVGANGTGKSTLL
                     KMLGGIESLDSGSITVMRGLTTGYLPQDGLTLSGRSVFAECMSVFSNLRDIEEEMEAL
                     MQRMAEIDPASAEYRQVAERFHNIESEFRARDGYSIEAQAGIVLNGLGFLKRDWRRRT
                     EEFSGGWQMRIALAKLLLEKPDLLLLDEPTNHLDLEARNWLEQYLLSYPGAFVLISHD
                     RYFLDVTVAKIAEIWNKQVYFYTGNYDKYLSQKAERRAQLEAAWKNQQEKIHHLETFI
                     NRFRYQATKAKQVQSRIKELDRIERIEIPPEEKTIHFRFPQPKASGRVVAEFIGVAKS
                     YPGVDVFRDVSFIVERGDRIALVGVNGAGKSTLIKLLAGIEPLTSGEYRLGHNVEPDY
                     FAQDQYKELDPAARMLDDLSAIAQRSTQTELRNLLGCFLFSEDDVFKPIGVLSGGERN
                     RYALARMLLSPSNFLLLDEPTNHLDLRAKDVLLNALQEYSGAVIFVSHDRYFIDKLAT
                     RVFEVGDCAVRVYPGNYEDYQWRKQAQAEPPPASAAEEELAQAASPEPDGAKQKRLNP
                     IRLRQLQEQCGSLEERISRAEAEIAESEASLAVFVSVEETLRLTQLVESRRAELESLM
                     QEWESVSAQIQENSPA"
     gene            complement(12493..>12886)
                     /locus_tag="VFQ79_18435"
     CDS             complement(12493..>12886)
                     /locus_tag="VFQ79_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0141707.1"
                     /translation="ILRYPNTAVASDGGVQEPGVGVPHPRSYGTNARVLAEFVRVRKV
                     LTLEDAIRRMTSLPARTFGFRDRGRILEGMAADLVLFDPDTVQDKATFQEPHQFSEGF
                     EMVLVNGIPVVDQSQPTGTLPGHVLRRQ"
BASE COUNT         2596 a         3926 c         3790 g         2574 t
ORIGIN      
        1 ggtcgctcgc gcgcctcacg atgacgttca atccacaggt ctgaccaatc tcctttacgg
       61 ggtctgcgcc ccactaaacc gatacagcct gtgacctgtc tactccggaa gtcgcctccc
      121 ggagcttacc gccgaccgta gcgccatgag acattcatct catctccttt ctgcgccaga
      181 ggagccatca tcggctcctg gctgttcgtt gtgtaatgcg aaaagacgcg gagaataccc
      241 gcattagtcc caacgccgtt cacttaagga atttggggct gcgcaaggtt cgaattggcg
      301 tctcagtcaa gcctaccttc ttccgagccg gtaggagtca gggcggagcc ataagtccag
      361 tttgggcgtt gggaataaag ccgatggtgt ggcgtacgcc tgcgccactt ctcagcctct
      421 gagcgttggg cacttccgca caatcgtcgg caaatctcag cagcaggcgt tttccgtcca
      481 ggtgacaggc tgccactgca tgacgatttc gtaagcgctg gccgactact cgcggtgatt
      541 accgggccaa gctgcggctg ccggcacgac tcgccgactg tacgctcaat cggatatttc
      601 ttagattcgt cgataatcgg gacaaccgtg cggatctact cttgacgcag tgccctgact
      661 ggatcgaccc ggcttgcgcg acgggcaggt atgaaacctg cgaccaccgc ggctgctaac
      721 aaaatcgtaa tcatagctgt caacgtcgca acctgaacag gctgcattcc ttgcacgaga
      781 cgctgcagta cgcgtcctgc tgctagagca gcgagtatcc ccaaaccaat gcccgcaaat
      841 gccatctgca agcattggcg caacaccagc gccatcacat cgcgaacggt tgctccaagc
      901 gccatgcgca caccaatctc aggtacgcgc tggccggtca agtaggccag cacgccataa
      961 atgccggtcg ccgcgagaat caaagcgatt ccggcaaaca ccccaaatag aagcaagaga
     1021 aaccggtgcc gggcgagcga ggcgctcgcg agctgctcca tcgttcggag ttcatatagc
     1081 gcctggtctc tagccgcgcc ccgcaactcg aactccagcg gcttgattac atccatgggc
     1141 ggtattttcg ttcgcacggt aatggacatg atcgacgaga agaagcgcaa cagagggacg
     1201 ggcacctgcg caaagggata gtaaatctga tcgcgcacac gcgactgatc atcacgcgcc
     1261 aggccccagt gccgcacatg gccaaccaca ccaacaacct gaacgggagc gtcgccaaac
     1321 gccggaatcc agagacgcct gccaacagcg tgctgttcgc caaaagcgtt ccgcgccaaa
     1381 ttctcatcga tgactacgac cgactcgctc ccttcgcgat cgtgttcatt gaagaaacgt
     1441 ccttcgcgca gcggaatccc catcaccttc aggtagtctg gtgttacgga tgaggcgagc
     1501 gcaaaaggct cccggttcgc cggcagtgga gccgccgtag tccggtaggt gactgaattc
     1561 tccccttcac gcatggggac gatatcggtg agcgcggcga attccacgcc gggcacacgg
     1621 cgagcgcggt caagcacgtc ctgccaggct gaccggattt gcgatggatt tgctaccgcg
     1681 ctgggggaaa gtgcaaagcg agccgtcaac acgttgtgca tattcaaacc gggatccagg
     1741 gaggagagcg tgagcaatgt cttccccaac atcccggcgc agactagcag cacgcacgcc
     1801 agcgcgatct cagagacaac gaatgcgcta tggaggcgcc gcgaacctcc ggcgagggcg
     1861 cgacctccgg cgcggagcgc gtgtcccagg ctgtgcgatg gaacacgcag agcggggacg
     1921 accccgaaca acaggccgct caagagcgac actccgatag caaaccagag tacagaccag
     1981 tcaagctgaa tctcctcggc gcggggcaag ctaccaggcc agaacaccac gaacggacga
     2041 aggcttaccg ctgcaaggac gatacccagc aggccaccgc aaataccgaa tgccacgctt
     2101 tcggtcaggc attctcgcat caggcggccc cgtccggcgc cgagcgcgac gcgcattgca
     2161 aattcgcgtt cacgtgaaat cgcccgagtc aggaacagac tggctatgtt cacacaggcg
     2221 agtaagagca gcaatccgac gccggacagg aggagccaca gtgtctcacg gacatctccg
     2281 accaactcct gttgcagcgg atgcacccgc atggtgatgc cttcattcga cttcggatac
     2341 tccttggcta gctgattcgc gatcaccgcg atctcggact gagcctcgga caacgtgacc
     2401 ttttgccgca ggcgcgctac gaggtgaata aagcgcgctg cgcgattttg catcctgggg
     2461 tctgtgctct gaccgagcgg cgtaaacacg tctgcatccc cagacaactg aaaacttgcc
     2521 ggggcaatgc cgacaaccgt ataggtctta ccctcaaaaa ccagtgttct gccgacggcg
     2581 gccggatcac ccgaaaaacg gcgttgccac aatgcgtaac tgatgattgt gacgggcggc
     2641 gccccgggcc ggtcttcgtc gggctgaaag gcgcgcccga gcaagggcag cgtgcccaga
     2701 gtcgggagca gttccgccga gatcttccgc ccgttcgcgt attccggttc tccaggctca
     2761 ctgatcgtgc cgccgccgta ggtccacgca gcaaccgtaa gcgatcggct gctacgcctg
     2821 agatcgtcga aatcgggatt cgagaacccc caaaagtcgc caaggcgctc ggtatatcca
     2881 ttcaccgtga ccagtctgtc cgagccggaa aacgggaggg ccttgagcag gacgctgtgg
     2941 accacggcga acatgacggt tgtggcggcg acgcccagcc cgaggatcat caccgcgacg
     3001 gcgccgaacc ccggcttttg cctgaaagta cgaaacgtat gacgagtgtc ccgcaccagg
     3061 tcctccagcc agcgtgtgcc gcgagcatcc ctgcactttt ccttgacttg ctcgaagcct
     3121 ccgagagaaa gccgggcttg tcgccgcgcc tcttcaggag tgcagccgct ggcgatcagg
     3181 tcgcccgcat gctggtcgag gtgaaagctc aattcctttt ccagttgctc ttccatttgc
     3241 ttccggccaa cgagacggga ccaccagttc atttcgctct cccgtcggcc atcgccagga
     3301 tgaatccgac agcttcggtc aaccgctgcc agctcgctgt ctcagcctcg agctgtgtgt
     3361 ggccctttcg cgttagccgg tagaacttcg cgtcgcgccc ggtttccgtt tttttccagt
     3421 ccgcggtgag aagccctcga ttctccagcc gatgcagcgc ggggtagaga gaaccttccg
     3481 gaacctggac cacaccgcgc gagacttgtt cgagccgttg cgcgatggcg tatccatgga
     3541 gagggccggc ggcaaccgct ttgaggatca gcaggtccaa tgtgccctga ggaagatccg
     3601 atttgggagt cgtcacatat acctcgtgct tctatggatg cattgtagcg caagaagcca
     3661 tagaagcaca aggtatgtga ttgtccgtcg acatccccgc atgtgtccgg ttccccggtt
     3721 tagttgcgca aagcggtgcc acgtgggatg tggcctgacg acagcctgat cctcgctgac
     3781 ggcgtagtat ttcacattcc tgacggtcgg tagttcgccg ccggaaggcg cgagggacag
     3841 gcgattaagc ggccatctcc cgtagcactt ctggatgcct ttctgcaacc cgaatcaagg
     3901 tcttcgccgc accgctggga acacggcgtc cttgctccca ttgctccagt gtgcgacgcg
     3961 atacgccaag caacgcagcg aactgctcct gtgtcaactc cgacttcagg cgcgccctaa
     4021 cgattggcga acggggctgg accgaagttc gttttccgcg gccggccttg atcgccgcaa
     4081 ctccttccgc gacctcttgc cagacgtcgc ggctggcccc caatcgggcc agtgcccgcg
     4141 cggtcagttt cttagtcatg ctcgaaagtc tccttgcagt tgtttgagaa tgtgggcggg
     4201 aacattctcg gtcttcgcct tcgcatagag tgttagcatc caaaactggt tcggctcata
     4261 gcgcacgtag tagattacgc gcaacccgcc acgctttcct atcccgccta gccgccagcg
     4321 aagttttcgc ataccacccg aaccagggac gacttttccg gcttccggat gtcgcatcag
     4381 gaactgttgc aactcgccaa attcgtcgtc atcgaggtag tacgcccgga accgttcgaa
     4441 ggcgggcgac tcgataaact cgaacacctc tctatactac gcctttggcg tatctccgtc
     4501 aaacagcacc gcgcgtccat gcacgtcgcc cgcctgcccg ttcatgttgc cggttgacca
     4561 ggatctcgaa ccgcttttat accgtgccgg ctagctcgcg atcaacggcg aagggtttac
     4621 gggatccagg ctgacgggtt gtcgacatag agtggaggtg ccctcgggcg ctcacctgat
     4681 ccgatccgac gccttttcat taagtgaacc gtattggcat tagtccggct tggcgtgagg
     4741 cttgtcaccc tgtgttcgcg gcaccataat agaggcgaaa tacgacgttg ccgaaagctg
     4801 cgatggaaga atcgcgtgag attacgggtc tcctccatgc ctggaggagt ggagacgcga
     4861 ccgcgctaga caagataatt ccgtgcgtct acgccgaact ccaccgcctc gctcatagtt
     4921 atctttcccg cgagcaatcg cgcgacgtgt tgcagactac ggcgctggtg cacgaagcat
     4981 acctccgcct cgtgcgggca agcgagatcg actggaaaag ccgcatccac ttctttgcca
     5041 tttccgccac tctgatgcgg cgaattctgg tagagtttgc aaggacgcgc cgtgccgtga
     5101 aacggggcgg taacgaacac cgggatgtct tcgacgaaga tgtcccgatg gcagtccccg
     5161 ttcaggaaga cttggttgcc atcgacgaag ccctgaccgc gctttccgaa atcgatccgc
     5221 gggaggccag ggtcgtggaa ctgcgtttct tcagcggaat gacggtggaa gaaatctccg
     5281 cggaactcga tatttcccca gacacggtat tgcgggactg gatgcacgcg aaggtatggc
     5341 tactccgcaa gttgtcgctg gagacgcgcc gtggaggatg agcgctggaa tcggagtaag
     5401 gacctgtacg cggctacgct caggatcgag ccagggcaga gacgagcatt ccttgctcat
     5461 gcctgcggaa ccgaccagag cctccggcgt gaactcaata ccctgctcga ttatgagtct
     5521 cgtgcccgca acttcttaga gctacccgcc gtagacgtca gggccaagtt tcgctccggc
     5581 ccaaccgatg ctcccgatat cgttgggacg tcactaggcc actatcaggt gctcgagaaa
     5641 gccggtacgg gaggaatggg tgtggtctac cgtgcctacg accggcaact cgagcgtgat
     5701 gtggcaatca agctcctggc gggcgagccg gaaacgactt cccgcatgcg gcttctggat
     5761 gaggctcgcg cggccgcgag actggctcat ccgggtattt gcgccgtata cggcgtaacc
     5821 tcggcaaacg gacacgcctg catcgtcatg gaattcgtcc ccggcgaacc tctggccgat
     5881 ttgctaaaga acggaccgtt aggcacggat gaagtgatcc ggttcggagg tcaactcgcc
     5941 gaagccttgg cacacgctca tgaacgtggc gtgatacacg gggacataaa gagtgcaaac
     6001 atcctcgtca caccggaacg gcggatcaag attctcgatt tcggtctagc gcggcggatg
     6061 cgtccggcga cgtcaccaac tcccggccca gccttcccga tgggtgcgct ggccggcaca
     6121 gttgcctata tggcgccgga actgctcctc ggccgggaac ccgatgtcca cagtgatatc
     6181 tggtcgctcg gagtcgtact gtatgagatg gcggcggggt ttcggccttt ctccgggccc
     6241 ggcactctcg aagtatgctc gacgattttg aattcgccgc accaaccgct gcccggccgc
     6301 gtaccagaac cgctccggag cgtgatcgag cgatgtctcg ccaaggaacc cgagcggcgc
     6361 tttcaacacg cgctcgaact gggatccgaa ctgcacaccg tcccgtggcg tgcggcggtg
     6421 acgcgagctt tgtccagaaa gatcgcggtg ttgccattcg agaatctccc ggtcggcggt
     6481 gcgaccgggc atctggcgca gggaattcac gatgccgtga tcacagacct ctaccgattg
     6541 agcggcttgc gggtgatcgc gcggcattcc tcgatggccc tcgggaaaga gcatttgagc
     6601 cgggttccag cgcggctgaa tggggtcgat ggcgtcatga ccggcacgat ctcgatagca
     6661 ggtgacacta ttgaattgag tatccagctt gcagatgcaa cgagtggtga gatcgtgtgg
     6721 gcgaatcgat acaacagctt actggccgat actgcgcggg tacgtaagga catcgttgcc
     6781 agagtgtgcg aggcaatgcg cctgcaatgc gatgcccagc aggcgcaggc cggaatcacg
     6841 cgggtcaagc ccgaaacgtc cgctctttac atgaaagcga gattcttcct taacaagctg
     6901 actccggacg gcttggcgaa aggtctggcc tatctgagaa ccgccgttga ggcagacccc
     6961 ggcgaacctc ttgttcacgc ggggttggcc ctcgcctacg ttctcatcgc tcatcaggcg
     7021 tccgtcaggc ttgtaggcga tccgttcgaa ctggcaaagc cgtatgcggc aaaggccatc
     7081 catctgaatc ctcagcttcc ggaagcccat caggcgcttg cggagatcag aacttacctg
     7141 gattgggact tcgccgcagc ggaacatgag taccggacgg cgctcgctct cagcaatgac
     7201 aacctcgccg aagcccacgc gcactactcc tggtatctca tgctcgctgg ccgcgagagc
     7261 gagcagatgg cccatatgaa gatggcgcag gaactcgatc ctctgaaccc cgtatatcac
     7321 gtttggatgg gccagcacta cgcgatcgcg ggaagatgga ccgacgtaac gccggaggcc
     7381 caagccgcat tggagatcaa tccgggaacg ccggatgcgt tagtgttgct tggtcacgca
     7441 tacctgcaaa gggggatgat cgcagagggg atcgagtgtc aggagcaggc tgcggcaatc
     7501 gatcttcggc tcagcacgta cgcgctcgcg gccacgtatg caaggattgg ccgggccgat
     7561 gacgctcgcc ggatcgcggc agaactacgc aagaacccag accattggaa tgcatacgga
     7621 cttgcctcta tttacgcggc gttgaacgac gccgatgagg cgctgttctg gctggaacgg
     7681 atgtacgcat cgcggcacat gtatactccc tgggtaagga tagcgccgga atgggcatcg
     7741 ttacgggacg atgctcgatt tcagaaattg gcgcaccgtc tgccagttcc gatcggggtc
     7801 acatggcctg atcaatgacg aacgtggaat gtgcatagcg cacaagcaca gggtcgcaat
     7861 gtttcgctac tagttcgccg tcagccgcaa ggcgatgtct tcggaatact ccggcgaatc
     7921 cagggttgtt tttcctccgg ggtgccgcag tgtgctcgac ttctctacct tggccgattt
     7981 cggattcacc cacatcatgt gataggtccc cgcggggagt tcgagttcca gcgccagttg
     8041 gtgcggggtg gcgtcaacct ggtagcgcgg cttggcgtct ttcacaacgc gcccgtgatg
     8101 gatatagacg gcataggatt ttcccggctc caccagcgcc tgaaaagaag cattttccgg
     8161 caatcctccc gctaaaatgc tgcggtcggg cttcatgtgt atgaagtcga tacgatccat
     8221 aaagtcatgc aggtttcgca ggtaccggcg caaacgggtg ctcccaccgc cgggctgagc
     8281 cgaaggatat tgaaaggtcc catcttcgct gcccgccgcg aaggagtaat ccagattgtt
     8341 atagagcgca cctccggcca gaaggaactc ccaaccctga atccggtaag tgtcatcttc
     8401 cactccgtca aagccggttt cattgttgcc gatggctttg ttcaggctgt aattcatcgc
     8461 cacgctttcg ggcggccgcg aatagtgaaa gttgaagata gagacgagag gattggggtt
     8521 ttcaatcttc ctggagccgt tcgcgatgtt ctgggcgatt aagtgccgct tgggcagcgt
     8581 cttttccgtt tcggcgatca caccggcgat gtgcgcctgc cattccaggg tcacgccgcc
     8641 gaagtacggt tcgttgcaga tctcgtagta gaggttgtcg aacccgttca actcggtgac
     8701 gatcttcttc accattgcct cctgcacctt caccaatccg ggatgacgga gagtgagaac
     8761 ctcttcccgc cctacgtttc cgaggtcgtt gatgttgttg cgggcattga gcgggctcac
     8821 ctcccacatt tcgtctttgt agtacgggca gaaaagcacc agttccacca ccacatcgtg
     8881 atcgttcgct ttccgcaaga aatccttcag ccgcgcgaaa tacgcctggt cccatttcgt
     8941 caggtcgaac ttgtctcccg cgcgctgcca cggcgaaatg aaccggctcg gcttgggtgc
     9001 cagggtgttg gaggcgatct ggaacgaacc ggggacttcg ctatacacac cggtaaacgt
     9061 ccgcgtcaga ttcaggcggt cgcggttcaa agtctccagg tatcgggtgt aattgaaatc
     9121 gagattcagc actgctccat agtgttctcc ggatgtgatt aaaacggccg gcttgccgcg
     9181 aaaaagaaag tagtgaggat tctccggatg gaggcggagg ggttccgcca ccagggcggc
     9241 aagggaaacc actcctgtga gcaagagccg tgcaatcaca ataacctcct ggtgcgcggt
     9301 acggcgaggc gccgggcaag ccgctgctcg cgatgccgca gggggttcga atcttcgcac
     9361 gcgagcgccg cccgggtcat ctccagagcc atcgtgtagt tccgctcccg gtgttcataa
     9421 tacttcgcca gatcctccag cgcggcgacg cggaaaggat tgcggcaggc cgacagttcg
     9481 gtaagcaccg caatggcagc gtccatgcgt ccgagttttc tctccagttg tgccacttcc
     9541 catagcgtcc ggaataacag gtcgtcgggc agcccggcat ccacggcgcg tcggaacagc
     9601 aggagcgcgt cttccatccg gccgcatttc cgcagccagc gcccgatccc caccatttcc
     9661 ggtccgcgcc tcaggcgtgc ctcggccggg gagcgaaacg cccacggcac aatcgcacac
     9721 aaacaggcca gggtcagaat atcgatggca ttgtggtgaa aaatcggcgc tacgcgaaag
     9781 gcctcacggc gccgcagata ctcaaagtag acgtacggaa tcatctcgcc cgggagatcc
     9841 ccctcgcgct ccactcccag cacctggttt tcgagatcca ccaggcggca gctttcgaat
     9901 tgaagcttcc acaagcggcg cgccccgtac agcagatcga cgtgccccat gtggtcgaat
     9961 ggcgaccttc gccgcgacat gcggtagcgg gtttcgagca ggggctgatc atacgctctt
    10021 ccgttgtacg tcaccagcac cgaaaactcc gacaggtacc gttcgagtgc gaacaacagg
    10081 ctggcttctt cggcgtaatc cctcatgaag aactgccgca ggtgaaaccc ttccgcggtg
    10141 atccggccca cgccgatgag aaacgcgtat gtgcccgacc cgccggcgag gccggtcgtt
    10201 tccgtatcca gaaaggccca gtggtgcgga gcagccgggt ccacggcgcg cccagtcagc
    10261 tcgtcgaaca ggtccggagg caaatcgttc aaatccgata tgccgatgct gccgtgccgg
    10321 cggtggcgct cccaaacccg ctcggtttca aagtgacggc catgcggggt ttccacctcc
    10381 tggccgctca gccagtgttc gatggaatag cgctccggaa gccgccgctc cggggccggt
    10441 gactgcggcc gggccgtggc gcccgcatat ttccggtcta tgcgtgcaat gcggcggcgc
    10501 aactgctgga gttgatcctg gagatccatc tggcggattc gctttttctt tgccccaatg
    10561 atatcacgcc ggcgaattct cctggatctg tgcgctgacg ctctcccact cctgcattag
    10621 ggactcgagt tccgcccgcc tggactcgac cagttgcgtg agccgcagcg tttcctcaac
    10681 gctgacaaag acggcaaggg aggcttcgga ttccgcgatt tcggcttccg cacggctgat
    10741 gcgttcttcc agggaaccgc actgctcctg caattgccgc agccggatgg gattcaggcg
    10801 tttttgcttc gcgccgtccg gttccggcga agctgcctgt gcgagttctt cttccgcggc
    10861 ggaggcgggc ggcggctcgg cctgcgcctg cttcctccac tgatagtctt cgtaattacc
    10921 cgggtataca cgcaccgcgc aatcgcctac ctcgaagacc cgtgtggcga gcttatcgat
    10981 gaaatagcgg tcgtgcgaaa cgaagatgac ggctccggaa tactcctgca aggcgttcag
    11041 cagcacatcc ttcgctctca ggtccaggtg attcgtcggc tcgtcgagca gcaggaaatt
    11101 cgaggggctc aacagcattc gcgccagcgc gtagcggttg cgttcgcctc cggagagcac
    11161 gccgatcggt ttgaagacgt catcttcgga gaacagaaag cagcccagca ggttccggag
    11221 ttccgtctgc gtggagcgct gcgcgatggc cgatagatcg tccagcatgc gcgccgccgg
    11281 gtcgagctcc ttgtattgat cctgcgcgaa gtaatccggc tccacgttat gcccgaggcg
    11341 gtactcgccg ctggtcaagg gctcgatccc ggccagcagc ttgatcaacg ttgatttacc
    11401 cgcgccattg acgcccacca gcgcgatgcg gtccccacgc tcgacgatga agctcacatc
    11461 ccggaaaacg tcgacacccg ggtagctctt ggcgacgccg atgaactcgg ccactacgcg
    11521 cccgctcgcc ttgggttgtg gaaagcggaa gtgaatcgtc ttttcttccg gcgggatctc
    11581 tatgcgttca atgcggtcga gttctttgat tcggctctgc acctgcttcg ctttggtggc
    11641 ctggtagcgg aaacggttga tgaaggtttc aagatgatgg attttctcct gctgattttt
    11701 ccacgccgct tccagttgcg cgcgccgctc cgctttctgc gaaaggtact tgtcgtaatt
    11761 gcccgtgtag aagtagacct gcttgttcca gatctcagcg atcttcgcga cggtcacatc
    11821 caggaaataa cggtcgtgcg aaatcagcac gaacgcgccg gggtacgaca gcagatattg
    11881 ttcgagccag ttgcgcgctt cgagatcgag atggttcgtg ggctcgtcca gcagaagcag
    11941 atccggtttt tcgagcagaa gcttggcgag ggcaatccgc atttgccagc cgccggaaaa
    12001 ctcctcggtg cgcctgcgcc aatcccgctt caaaaaaccg agcccgttca gcacaatccc
    12061 ggcctgggcc tcgatggaat atccgtcgcg cgcgcgaaac tcgctttcga tgttgtggaa
    12121 acgctccgcc acctggcggt attcggcgct cgccgggtcg atttcggcca ttcgctgcat
    12181 cagtgcctcc atctcctcct cgatgtcgcg gagattggag aacactgaca tgcactcggc
    12241 aaacacgcta cgcccggata gcgtcagccc gtcctgcggc agatagccgg tagtgagacc
    12301 gcgcatcacc gtgatcgatc ccgagtcgag cgattcgatg cctccgagca ttttaagaag
    12361 cgtggacttg cccgtgccgt tggcgccaac gagcccgacg cggtcgcggt tgccgatcag
    12421 ccagtcgagg ccttcaaaaa gtgtcttagg tccgaaacgc ttgcccgccg cggaaagctg
    12481 gatcatcgtt ccctactgcc ttcggagcac gtgtccaggg agcgtgccgg tcggctgcga
    12541 ctgatcgaca accggtattc cgttgacgag caccatctcg aagccctctg aaaactggtg
    12601 gggctcctgg aacgtggcct tgtcctggac agtgtccgga tcgaagagga ccagatccgc
    12661 cgccattcct tccaggatcc gtcccctgtc gcggaagccg aaggtgcgtg ccggcaacga
    12721 cgtcatgcgg cgtatggcgt cttcgagggt aagtaccttt cgtacgcgaa cgaactccgc
    12781 cagcacgcgc gcgttggtgc catacgagcg cggatgcggg acgccgacgc cgggttcctg
    12841 cacaccgcca tcgctggcca cggcggtatt ggggtatcgc aggata
//
LOCUS       DASTMK010000430         8302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_341588, whole genome shotgun sequence.
ACCESSION   DASTMK010000430 DASTMK010000000
VERSION     DASTMK010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341588"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="ftsZ"
                     /locus_tag="VFQ79_18440"
     CDS             complement(<1..1046)
                     /gene="ftsZ"
                     /locus_tag="VFQ79_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU0141708.1"
                     /translation="MEDKGLDALKFEIQEEALLGTRTKVVGVGGGGSNAVARMMAEGM
                     GGVDFYVLNTDRQALMASPVPNKLAIGTKLTNGLGTGSDPNIGREAALEDTERIIEIL
                     EGADMVFITAGLGGGTGTGAAPVVASLAKELNALTVAVVTKPFSFEGPRRMKQANAGL
                     SELASMVDTVIQIPNDRLLELAPRGTSVVDAFRNADDVLRQAVQGISDIITVPGLINR
                     DFSDIRTIMVGMGFAMMGTASAKGENAAVEAARLAINSPLLEEGGVRGARAMLVNITG
                     SSQLTLHDVNEACMLIRAAAEYDEVHLSFGVVMNEAMQDSVKITVIATGFEREGLPTI
                     ARRAKELAASDPFGT"
     gene            complement(1055..2251)
                     /gene="ftsA"
                     /locus_tag="VFQ79_18445"
     CDS             complement(1055..2251)
                     /gene="ftsA"
                     /locus_tag="VFQ79_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU0141709.1"
                     /translation="MGKPLLAVGIDPGSWRTRCVICRVDHEQLRLIGFGESYSRGWAK
                     GRVADQDTVSQCIVEAVREAESMARVQVESAVVGIGGAAIEGANSRGIYEFGRPREVE
                     PADLGYAADRATRIRLQEDRLLLQVCPQDFIVDGRAGYRNPKGIRCSRIEANIHVITA
                     AVQEHQFLLSALHQAHLAAEETIFEGMAAAYAATLQDDRCRGLAVVDIGDQSTEVAVY
                     DGEAMIGASSLPIGGERFTKDVAVCLCTSFEDASHLKEEYGCAILGLTSDNSLIELPS
                     AEGRPPREATRRQLNEVLEARAEELFRYIRWELARTGMEQSLLEGVVLAGGGALLNGM
                     CDVAEKILNCQTRNGLAVGIEDWPEYLDTPAWTVAAGLAMYSARLKLRKDLRRKAPGL
                     AGLVLR"
     gene            complement(2244..3065)
                     /locus_tag="VFQ79_18450"
     CDS             complement(2244..3065)
                     /locus_tag="VFQ79_18450"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU0141710.1"
                     /translation="MSSKPVSKVGPRSAWRYFLPGVISMSALFGGVFTFQRVEQYLIA
                     NPRFRFEAPAEYGEDAPGLRIEEISHASKVRLMQVFAPDFGRSVYLMPLAERRRNLLA
                     VNWVKDASVSRIWPHDLVVRISEREPVAFLTTARADRRQPFRYRLIDADGFILDPPPR
                     VSFHLPVVAGIGVNDELESRRQRIRKCLRFFKEIGPMGAKLSEVDVGDLGNLKATWQV
                     DEGVAVLMLGGADFAGKLKNFEKHYPEIRTRFPQAAMFDLRIEDRITVVGGAGNG"
     gene            complement(3058..4473)
                     /gene="murC"
                     /locus_tag="VFQ79_18455"
     CDS             complement(3058..4473)
                     /gene="murC"
                     /locus_tag="VFQ79_18455"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU0141711.1"
                     /translation="MFFKPQHLHFTGIGGIGMSSIAEVLLNLGYKISGSDLRLSPLTE
                     RLVGLGATVYEGHAPENLSGAKALVVSSAVNPNNPEVLEAKRLGIPIIPRGEMLAELM
                     RLKYGIAVAGSHGKTTTTSMVATILNQANLDPTVVVGGKVATMGGSNARVGHSDFLVV
                     ESDESDGSFLKLAPILAIVTNIDREHLDHYSSIEEIRAAFCEFVNKVPFYGMAILCLD
                     DENVQQILPEVNRRVLTYGTSAQADLAVVDVCCADFSSEFRLRLRDRDLGTFSIGVPG
                     IHNVLNATAAVAVGLELEVKPDVIRAGLAGFSGVDRRFQIRGKERGVTVIDDYGHHPT
                     EIRATLEAARLCRFAQIHVIFQPHRYSRTMHLMDEFGRSFHNADTVRVLDVYAASEKP
                     IEGVSGEILAERLRAFGHKGVEYTPDIDSAVASAAAVVKDGDVVLTLGAGNVWQAGDR
                     LLERLREGALNVEAGQERRNV"
     gene            complement(4564..5628)
                     /gene="murG"
                     /locus_tag="VFQ79_18460"
     CDS             complement(4564..5628)
                     /gene="murG"
                     /locus_tag="VFQ79_18460"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEU0141712.1"
                     /translation="MSVFLMAGGGTGGHVIPLLAVARELRKRGHSPIFVGTRRGLEAK
                     LVPPESFPLEWIEIGGLNRVGFRQILRTLCQLPISTLHAFRLISRYRAAAVFSLGGYV
                     GGPVTAAAWLHRIPMVLMEPNAIPGFANRRIARFVTKALVNFEETAKYFPAGRTIHTG
                     LPVREEFFSLPVKPRGETFSLLITGGSRGSRTLNQAARDSWRLFRESGIRIRIVHQTG
                     PEAFEEIRAEFARSGLEGEIVPFIADMPAAFAEADLVVCRAGAGAVSELAAAGKPSIL
                     SPFPFAADQHQLRNAEAFSRVGAALLVLDRDMTGAHLFKEVTRLASTPGVLERMSAAA
                     RSMARPHAAHRAADLLEELA"
     gene            complement(5625..6767)
                     /locus_tag="VFQ79_18465"
     CDS             complement(5625..6767)
                     /locus_tag="VFQ79_18465"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU0141713.1"
                     /translation="MAQRLKTDWTLFCTVILMVTFGLVIIYSASSMIATLKYNLPSHY
                     FLLRQAAWALVGFCALMYFKRKDYRLLSAPHWAFVPMGLVLVMLLAVIFLDPLAHRWL
                     RVGPLSLQPSEFAKPALAIFLAWFITRRAAAINNVHTWLPAGLALLLLAGTVLVGDLG
                     TAAVLAVTGAAVFYVAGLNRRYFILALTGGVLLAGFAVLAKPYRLGRIIGYVDPEFKL
                     IDKIDPAGRIKSYAHSTLSTRDPSYQARQSKIALGSGGPLGLGLMQGKQKILYLPEAH
                     TDFIYAVIGEELGIWGCAGVLFAFLIILWRGLRLYFVAPDDFGRYLALSVTVTVAFQA
                     LINMSVVLDLVPTKGIPLPMISHGGSSLLSTLMSLGILLSVSEHTG"
     gene            complement(6767..8113)
                     /gene="murD"
                     /locus_tag="VFQ79_18470"
     CDS             complement(6767..8113)
                     /gene="murD"
                     /locus_tag="VFQ79_18470"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU0141714.1"
                     /translation="MELKGSNVLVVGMARSGLAAARLLVQHGAHVRAADAKPMESLHG
                     AERELESLGVSFTPQSPAAFEGCDLIVISPGVPRDLDLLEQARRRGTPVIGEVELAGW
                     YLRGKTLGITGSNGKTTTTALAGHILRECGIPVQVGGNIGTAPASMVDSSREGQWNVL
                     ELSSFQLETVDRFRAHIAVITNLTPDHLDRHHTMEAYVEAKGRLLVNQTAQDYAVLNA
                     DDATCAAFSDSIRSRLVWFRGSRRTETGWSFDGAVIFFEGRPWMEASGIPLRGRHNIE
                     NVMAAAGATHVAGAPLEQIAEAVGSFPGVEHRLEFTAEIAGVQFFNDSKATNVDATLK
                     AIDAFDGGLWIILGGKDKGSDYTPLIEPLRRKARAALLIGAAADKIASHLGNAVPVIR
                     SGTLESAVRTAVERASSGDTVLLAPACASFDQFESFEHRGRVFKEIVHSLESAGVS"
     gene            complement(8119..>8302)
                     /gene="mraY"
                     /locus_tag="VFQ79_18475"
     CDS             complement(8119..>8302)
                     /gene="mraY"
                     /locus_tag="VFQ79_18475"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719701.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU0141715.1"
                     /translation="ALSVIIQVGSFKIRGKRVFKMAPLHHHFEALGWQESKIIARFWI
                     LGLVMALFALTTLKLR"
BASE COUNT         1657 a         2773 c         2329 g         1543 t
ORIGIN      
        1 gtgccgaacg ggtcggaggc ggccagttct ttcgcccggc gggctatcgt aggtaagcct
       61 tctctttcga aaccagtggc gatgacggtg atcttgacgg aatcctgcat tgcctcattc
      121 atgacgacac cgaagctgag gtgaacctca tcgtattcgg cggcggcgcg gatgagcatg
      181 caggcttcgt ttacatcgtg caatgtgagt tgcgaagaac cggtgatgtt gaccagcatc
      241 gcccgggcgc ctcgcacgcc accctcttcc agcagaggtg agttgatggc caaccgggcc
      301 gcttcaacgg cagcattctc ccccttcgca gaggcggtcc ccatcatcgc gaagcccatg
      361 cccaccatga tggtgcggat atccgaaaaa tcgcgattga tcaagccggg cacggtaatg
      421 atatcgctaa taccctgcac cgcctggcgc agcacgtcgt ccgcattccg gaatgcgtcg
      481 acaacagagg ttcctctggg agcaagctcc agcagccggt cgttggggat ctggatcacc
      541 gtgtccacca tggaagccag ttcgctcaat ccggcattcg cctgcttcat gcggcgcggt
      601 ccctcaaagc tgaaaggctt ggtgaccacg gccacggtga gggcattcaa ttctttcgca
      661 agcgaagcca ccacgggcgc ggcccctgtc ccggttccac ctccgagccc cgccgtgata
      721 aacaccatgt cggcgccttc cagaatttcg atgatgcgct cggtatcttc gagcgccgcc
      781 tcacggccga tgttcgggtc cgagccggtt cccagaccgt tagtcagctt cgttccgatc
      841 gccagtttgt ttgggaccgg agaggccatc agtgcctgcc ggtccgtatt gagcacgtaa
      901 aaatcgacgc cgcccattcc ttccgccatc atgcgcgcga ccgcattgga accgcctccg
      961 cccacgccta ccactttggt gcgcgtgccg agcagcgcct cttcctgaat ctcgaacttc
     1021 aacgcgtcca agcccttgtc ttccatttgc cctcctagcg taaaaccagc ccggccagac
     1081 cgggcgcctt gcgtctcaga tcttttcgga gcttcaatcg ggccgagtac atcgccagac
     1141 ctgccgccac ggtccacgcc ggcgtatcca ggtattccgg ccaatcctca atccccacag
     1201 cgagtccatt gcgggtctgg caattgagta tcttttccgc cacgtcgcac atgccgttca
     1261 gcagcgcacc ccctcccgcc agcacaacgc cctcgagcag cgattgctcc attcccgtcc
     1321 tcgccagctc ccagcggatg tagcggaaca gttcttcggc tctggcttcc agcacttcgt
     1381 tcaactggcg gcgcgtagct tcgcggggcg gccggccttc cgcgctcggc aattcgatga
     1441 ggctgttgtc cgaagtcaaa cccaaaatcg cacagccgta ttcttccttc aaatgggagg
     1501 catcttcaaa tgaggtgcac aggcatacgg caacgtcctt ggtgaaccgc tcgccgccga
     1561 taggcaggct ggacgcgcca atcatggctt ccccgtcgta caccgcaacc tcggtagact
     1621 ggtctccgat atccacgacc gcgagtccgc ggcagcggtc atcctgcaac gtggccgcat
     1681 aggcggccgc catgccttca aaaatggtct cctccgcagc caggtgcgcc tgatgcagcg
     1741 ccgaaagcaa aaattggtgt tcctggaccg ccgcggtaat cacgtggatg ttcgcttcga
     1801 ttcgcgagca gcggatgcct ttgggattgc gatagcccgc acggccgtcg acaataaaat
     1861 cctgcgggca aacctgcaac agcagccggt cttcctgtaa gcggatgcgg gtagcgcggt
     1921 ctgccgcgta accaaggtcc gccggttcca cctcgcgcgg ccgtccaaac tcgtagatgc
     1981 cgcggctgtt ggctccctcg atggcggcgc cgccaatgcc gacgacggcc gattccacct
     2041 gcacgcgggc catcgattcg gcttcgcgga ctgcctcgac gatgcactgg gagaccgtat
     2101 cctggtcggc aacgcggcct ttcgcccagc ctcgcgaata ggattcgccg aagccaatca
     2161 atcgcaactg ctcgtgatca acacggcaaa tgacgcaacg cgtccgccaa ctcccggggt
     2221 cgatcccgac cgccagcaga ggcttaccca ttccctgctc ctccaaccac ggtaattcga
     2281 tcctcgattc gtaagtcgaa catagcggct tgcggaaacc ttgtccggat ctccggataa
     2341 tgcttttcaa aattcttcag tttcccggca aaatccgctc cgcccagcat cagcaccgcg
     2401 acaccttcat ccacctgcca ggtcgccttc aggttgccca ggtccccgac gtcgacctcg
     2461 gataactttg cgcccatcgg tccgatctct ttaaagaacc tgaggcattt ccggatccgc
     2521 tgccgccggc tttccaactc atcgttaaca ccgataccgg ctacgacggg caggtgaaag
     2581 gagacgcgtg gaggcggatc cagaatgaac ccatccgcgt cgatcaggcg atagcggaac
     2641 ggctgccgcc ggtccgcgcg ggccgttgtg agaaacgcaa ccggctcccg ctcgctgatg
     2701 cgcaccacca ggtcgtgagg ccagatgcgc gacacactcg cgtccttcac ccaattcacc
     2761 gccagcaggt ttcggcggcg ctccgcaagg ggcatcaaat agacgctgcg gccgaaatcc
     2821 ggcgcaaata cctgcatgag gcgaactttc gacgcgtgcg aaatttcttc gatccggagg
     2881 ccaggcgcat cttcgccgta ttcggccggt gcttcgaagc gaaaccgcgg gttcgcgatc
     2941 agatactgct cgacacgttg aaacgtgaac acgcctccga aaagcgcgga catggatatc
     3001 acacccggca ggaagtatct ccaggcgctt ctgggaccca ctttcgagac cggtttgcta
     3061 gacattgcgc ctctcctgcc ccgcttcgac attcagcgcg ccttcccgca gtctttcgag
     3121 caaccggtcg cccgcctgcc agacattccc ggcgccgagg gtcagcacca catcgccgtc
     3181 cttcaccacc gccgcggccg atgccaccgc gctgtcgatg tccggcgtat actccacgcc
     3241 tttatgtccg aaagcacgca ggcgctccgc cagaatttcg ccgctcaccc cttcaatagg
     3301 cttttccgaa gcggcgtaga cgtcgagcac gcgcaccgta tcggcattgt gaaacgagcg
     3361 gccaaactca tccatgagat gcatggtgcg gctgtagcgg tgcggttgga aaatcacgtg
     3421 aatctgtgcg aagcggcaca gccgcgccgc ttcgagcgtc gccctgattt ccgtcgggtg
     3481 gtggccgtaa tcgtcgataa ccgtgacgcc gcgctccttg ccgcggatct gaaaccggcg
     3541 gtccacgccg gaaaaacccg ccagccccgc gcggatcaca tcgggtttca cctcgagttc
     3601 caggcctacc gctaccgcgg ccgtcgcgtt gagcacgttg tggattcccg gcactccaat
     3661 gctgaacgtc ccgagatccc ggtcgcgcag gcgcagccgg aattcactcg aaaaatcggc
     3721 gcagcagacg tcgaccaccg ccaggtcggc ctgggcactg gttccgtagg tcaacacgcg
     3781 ccggttcacc tccggcagaa tctgctgcac gttctcatcg tccaggcaca atattgccat
     3841 gccgtaaaac ggcactttgt tcacgaactc gcagaatgcc gcgcgaatct cctcaatgga
     3901 ggaatagtga tcgaggtgct cgcgatcgat attggtgacg atggcgagaa tgggcgccag
     3961 cttcaggaac gatccgtcgc tttcatcgga ttccaccacc aggaaatcac tgtgtcccac
     4021 gcgggcgttc gatccgccca tggtcgccac tttaccgccc accaccaccg tgggatcgag
     4081 attcgcctgg ttcagaatgg tggccaccat cgacgtggtc gtggtctttc catgactccc
     4141 cgcgaccgcg atgccatact tcaggcgcat tagctcggcc agcatctctc cgcgggggat
     4201 gatgggaata ccgagccgct tcgcctccag aacttccgga ttgttggggt ttacggcgga
     4261 gcttaccacc agcgctttcg caccgctcag attctcgggc gcgtgcccct catagaccgt
     4321 ggcgcccaac ccgaccagcc gctccgtaag ggggctcagc ctcaggtcgg aaccgctgat
     4381 cttgtatccc aggttcaaca acacctcagc gatgctgctc atgccgatac caccgattcc
     4441 cgtaaaatgc agatgctgag gtttaaaaaa cattttacta ccgtattgtt ccgggtttat
     4501 ccgggcgtgt caataacatg ggaaaaaaac acgcgcgctt acgcacctgc cgttgtttgt
     4561 ggctcacgcc aactcctcca gcaggtccgc cgcccgatgc gccgcgtgcg gccgcgccat
     4621 cgaccgggca gcggcgctca tacgctcaag cactccaggc gtggaagcaa gcctcgtcac
     4681 ttccttgaac aaatgtgcac ccgtcatatc gcgatcgagc accagcagtg ccgcgcccac
     4741 ccgcgagaat gcctccgcat ttcgaagctg gtgctggtcg gccgcaaacg ggaagggcga
     4801 caggatggag ggtttgcctg ccgctgccag ctccgatacg gcgcccgctc ccgcgcggca
     4861 aaccaccagg tccgcctccg cgaaagccgc cggcatatcg gcgatgaacg gcacgatttc
     4921 tccttccagt ccggaccgtg cgaactccgc ccggatctcc tcgaaagctt ccggcccggt
     4981 ctggtggaca atcctgatcc ggattcccga ttcgcgaaac aggcgccaac tgtctctcgc
     5041 cgcctggttc aaagtccggg agccgcggct ccctcctgtg atcaacaggc tgaacgtctc
     5101 tcctcgcggc ttcaccggca acgagaagaa ctcttccctg accggcaacc ccgtatgtat
     5161 tgtgcgtccg gcagggaagt attttgccgt ctcttcgaag ttcaccaacg ctttcgtcac
     5221 gaaccgtgca atccggcgat tggcaaagcc cggaatagcg ttcggctcca tcagcaccat
     5281 gggaatgcgg tggagccatg cggcggcggt aaccggaccg ccgacatacc cgcccaggct
     5341 gaagaccgcc gccgcgcggt agcgcgagat cagcctgaac gcatgcagag tgctgatggg
     5401 caactgacac aacgtccgaa ggatctgccg gaacccgacg cgattcagcc cgccaatttc
     5461 aatccattcg agcgggaacg attccggagg aaccagcttc gcctccaatc cccggcgcgt
     5521 gcccacgaag atgggcgagt ggccgcgttt ccgcaactca cgggcgacag ccagcagggg
     5581 aatgacgtga cctccggtac ctccacccgc cattagaaaa acactcatcc ggtatgttcg
     5641 ctgacactca gcaggattcc gagcgacatc agcgtgctga gcagcgaact ccctccgtga
     5701 ctgatcatgg gcaggggaat ccctttcgtc ggcaccaggt ccagcacgac actcatgttg
     5761 atcaaggcct ggaacgcaac cgtgacggtt acacttagtg ccaggtagcg cccgaaatcg
     5821 tccggcgcga cgaaatagag ccgcagcccc cgccagagaa tgatcaggaa cgcgaacaac
     5881 actcccgcac atccccagat accgagttct tcgccgatca cggcgtaaat aaaatcggtg
     5941 tgcgcttcgg gaagatacag gatcttctgt ttgccctgca tgagcccgag gccgagcggc
     6001 ccgccggagc ccagggcgat cttcgactgc cgcgcctggt agctggggtc gcgcgtactc
     6061 aacgtgctgt gcgcatagct cttgatacgg cccgccggat cgattttgtc gatcagcttg
     6121 aactccgggt ccacgtaacc gatgatccgg ccaagccggt agggtttcgc caataccgca
     6181 aaaccggcca gcagcacgcc gcccgtcagc gccaggataa agtaccggcg attgagtccc
     6241 gccacgtaga aaaccgcagc ccccgtaacg gcgagcacgg cagccgtgcc aagatcgccg
     6301 accagcaccg taccggcaag cagcagcaac gccaggcccg cgggcagcca cgtgtgcacg
     6361 ttattaatcg ctgcggctct gcgggtgatg aaccaggcga gaaaaatcgc cagggccggc
     6421 ttggcaaatt ccgatggctg aagcgaaagc ggtcccaccc ggagccaccg gtgcgcaagc
     6481 gggtccagaa agatgaccgc gagcagcatc accagcacca gccccatggg cacaaaggcc
     6541 cagtggggtg cgctcaacag ccggtaatct ttccgcttga aatacatcag cgcgcaaaaa
     6601 ccgacgagcg cccacgccgc ctgccgcagc agaaagtagt ggctgggcag gttgtatttc
     6661 agcgtcgcga tcatggacga cgcgctgtaa atgattacga gcccgaacgt caccatcagg
     6721 atgacggtgc agaacagggt ccaatctgtc ttgaggcgct gcgccatcaa ctcacccccg
     6781 ccgattcgag cgaatgcact atctctttga agactcttcc tcggtgctcg aagctctcga
     6841 actgatcgaa gcttgcgcac gccggggcca gcagaaccgt gtcaccggag gaagcccttt
     6901 ccacggcggt gcgcactgcg gactccaacg ttccggagcg gattacaggt accgcatttc
     6961 ccaggtgcga cgcgatcttg tccgccgcgg cgccgatcaa aagcgccgcg cgcgccttgc
     7021 ggcgcagcgg ttcgatgaga ggcgtataat cgctcccttt gtccttccca ccgagaatga
     7081 tccaaagccc gccgtcgaag gcgtcgatcg ctttcaaagt ggcatctacg ttcgtagcct
     7141 tggagtcgtt gaagaattgc acgcccgcaa tttcagcggt gaactccagc cggtgctcca
     7201 ccccggggaa gctcccgaca gcttcggcaa tctgctcaag aggagcgcct gccacgtgag
     7261 tggcaccggc cgccgccatt acgttctcga tattgtggcg tccacgaagc ggaatgccgc
     7321 tcgcttccat ccagggcctg ccctcgaaga aaatgaccgc cccatcaaag gaccagcctg
     7381 tttccgtccg ccggctcccc cggaaccaga ccagccggct ccggatggaa tctgaaaaag
     7441 cggcacaggt ggcgtcatcc gcgttcagca cggcataatc ctgggcggtc tgattcacca
     7501 ggaggcggcc cttggcttcg acgtacgctt ccatggtgtg gtgccggtcc aggtgatccg
     7561 gcgtcagatt ggtgatcacc gcgatgtggg cgcggaaccg gtcaaccgtc tccaattgaa
     7621 aactggagag ttcgagtacg ttccactgtc cttcacggga actgtcgacc atggaggcgg
     7681 gagccgtgcc gatgttaccg cctacctgca ccgggatgcc acactcccgt agaatgtgtc
     7741 ccgccagagc ggtcgtagtt gtcttcccgt tcgagccggt gatgccgagc gtcttccccc
     7801 gcaggtacca cccggccagc tccacttcgc cgataacggg ggttccccgc cggcgcgcct
     7861 gttccagcag atccaggtcc ctggggacgc cgggagaaat gacgatcagg tcgcagccct
     7921 cgaaggccgc cgggctctga ggggtgaagg agacaccgag ggattcaagc tcccgttccg
     7981 cgccgtgcag gctttccatc ggcttggcat ccgcggcccg cacatgggcg ccatgctgca
     8041 ccagcagccg cgccgcggca agacccgacc gcgccatacc caccaccaat acgttcgagc
     8101 ccttcagttc cattcccgtc atctcagctt cagggttgtc agcgcgaaca gcgccatcac
     8161 cagcccgaga atccagaacc gggcgataat cttcgactcc tgccatccga gcgcttcgaa
     8221 atgatgatgc aggggagcca tcttgaagac acgtttgccg cgtattttga agctgcccac
     8281 ctggatgatg accgacagcg cc
//
LOCUS       DASTMK010000431         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_54608, whole genome shotgun sequence.
ACCESSION   DASTMK010000431 DASTMK010000000
VERSION     DASTMK010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54608"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..886
                     /locus_tag="VFQ79_18480"
     CDS             71..886
                     /locus_tag="VFQ79_18480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU0141716.1"
                     /translation="MASFLETAIEIAREAGSLLAHYFERRPAFELKGEADLVTEADRA
                     SEKLVVERLRSHFPAHSILAEEGGGHETQSEYRWYVDPLDGTTNFAHGYPVFNVTMAL
                     ERNGELIAGVIYDPIHQELFAGERGAGAYLNNRRIRVSSVRHLAQSLVATGFPSYKRH
                     LNINIHFYHQIAMASHGVRRGGSAALDFAYVACGRLEAFWEFGLKPWDMAAGIVIVTE
                     AGGKCSDMRGSAPTLGGANVAASNGHIHTELLEAFGDVFAGRFKIPLPEIKPA"
     gene            complement(963..3935)
                     /locus_tag="VFQ79_18485"
     CDS             complement(963..3935)
                     /locus_tag="VFQ79_18485"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0141717.1"
                     /translation="MFQRYLKLLVLFTSLSALVFAQGLQTNASKDDWEEINFEFNSAI
                     LSDGYPSLLRLAELLQQNPGYKVRLVGNADWIGGNRYNQKLGTSRAEEVKRFLVKYGA
                     SDGQVETATQGEESPKVPNRTKEGRFMNRRVEVNVFDDQGRRVAAGGVKEAIDVIPDW
                     AKKQQECCEEILKRLDRLDEIVSMLKDLGGVRADLEKLRKEHDDLRQQVAGLPKPLTR
                     DETTQVARTATAEEIEKARMPRFSISRLNFGADDNRDLTFTGMGRYFAPFKDRFAVQA
                     QGEYMYFRDRQEGQFDIGLVNRIHTRAQAGMFASFKHVTLREMQSGGTLGQASFTLDY
                     LFSHGRAGVFAAKGFMNDAIVNRVALTRNVFEETYLSTVDQYGASTTLGLFGENYVEG
                     NLGWLNSRGHGNRVGGTARFIFPISDRFAFTLEGGMNETLLARGNAGRVVAGFQFGNF
                     IRPKDYLGVDHAVPTDVPRIRYELLTRRVRTGNDPPVADAGADQVGVPAGPIQLDASG
                     SFDPEEDPITFQWSQVAGPAVTLSGMTSAVATFTAEEGQSYAFRVTVKDDKGAQSIDR
                     VSVITRSAPQVQIARFAANPAAIDAGQTSTIEWRVLNATEVNITNLGRVDANAGTSTV
                     SPADTTEYRLTAKNDTNEVSETITITVRRPEARFQTCTAQPMNIMQGESATLFWATQA
                     ADTVSISGLGNVDATGTRSVSPTESTTYTFTARNRFGETTCTVNVQVTEGQRPRILSF
                     AANPTEVVAGGTSALSWSVENATEVTIDGIGTVQNSGSRNVQPQQTTTYTLRARNNTG
                     EVTATATVTVTPGPVLPPTLASCVAAPATITTPGTAVSLSWAAQNATTVTIAPGVGEV
                     PVPGPFTVNPQQTTTYTITAAGVQGSTPATCQVTVTLQGEAPIPDIAGPDVIETIYRE
                     LSLDASGSTDPNGLPLTYIWEPMGTGVAILDQGQPITRIQVGGLFGDYPIRLTVRNSA
                     GQSATTTVTVRFKSSTIL"
     gene            4214..4572
                     /gene="rnpB"
                     /locus_tag="VFQ79_18490"
     ncRNA           4214..4572
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFQ79_18490"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          926 a         1361 c         1333 g          989 t
ORIGIN      
        1 gtttgagaat tgacgctccg gatcatgcga gcctcccatg cttcgcatgg ctgtccaata
       61 actccttaac atggcatctt tcctggaaac cgcgattgaa atcgctcgtg aagccggttc
      121 cctgctcgcg cattactttg agcggcggcc cgctttcgaa ttaaagggcg aagccgacct
      181 ggtgaccgaa gccgaccggg cttccgaaaa actggtggtg gaacgccttc gttctcactt
      241 cccggcgcat tctatattgg ccgaggaagg cggcggccac gagacccaat cggaataccg
      301 ctggtatgtc gacccgctgg atggcaccac caatttcgcg catgggtatc ccgtattcaa
      361 cgtgacgatg gcactcgaac ggaatggcga attaatagca ggcgtcattt acgaccccat
      421 ccaccaggaa ttattcgccg gcgagcgggg cgcaggcgcc tatctcaaca accggcgcat
      481 tcgtgtttcg agcgtccggc atcttgccca aagcctcgtt gctacggggt ttcccagcta
      541 caagcgccac ctgaatatca acatccattt ctatcatcag atcgcaatgg caagccacgg
      601 agtgcgccgc gggggttcgg ccgcgctcga cttcgcctac gtggcttgtg ggcggctgga
      661 agcgttttgg gaattcgggc tgaagccctg ggatatggcg gcggggatcg tcatcgtcac
      721 cgaggccggc gggaaatgct ccgatatgcg cggcagcgca cctacgctgg gtggcgcaaa
      781 cgtcgccgcc agcaacggcc acatccatac ggaactcctt gaggcgttcg gcgatgtctt
      841 tgccggccgc ttcaaaattc ctcttcctga aatcaagccc gcataaaaaa agcgggcggc
      901 gcattcacgc caacccgctt tttaagatcg gggcggcagt agccgcccct tttacctccg
      961 cttcaaagaa tggtgctgct cttgaagcga accgtcactg tagttgtggc gctctgtccg
     1021 gcgctgttac gcaccgtcag gcgaatgggg taatcgccaa agagaccgcc aacttgaata
     1081 cgagttatag gttgaccctg gtcgagtatt gcaactcccg tccccatggg ttcccagata
     1141 taggtcagag gcaggccatt cggatcggta gatccgctgg catcgagcga caactccctg
     1201 taaatggtct cgatcacgtc cggaccggca atatccggta tcggagcttc gccctgtagt
     1261 gtcaccgtca cctggcaggt agctggagta cttccctgga cacctgcggc tgtgatcgta
     1321 tacgttgtgg tctgctgcgg attcaccgtg aatggacccg gcaccggcac ttcgccgact
     1381 cccggtgcga tggtgacggt ggtcgcattc tgggcagccc agcttagcga taccgccgtg
     1441 cccggcgtgg tgatcgttgc cggagcggca acgcaggagg ccagtgtggg tggcagcacc
     1501 ggccctggtg ttactgtcac tgtcgcagtc gcggtaactt cacccgtgtt attgcgggcg
     1561 cgaagcgtat aagtggtcgt ctgctgcggc tgtacgttcc ggctgccgct gttttgaacc
     1621 gtgccgatgc catcgatagt cacctcggtc gcgttctcca cagaccagga caacgccgac
     1681 gtacccccgg ctacgacttc cgtcgggttc gccgcaaagc tgaggatacg cggccgctgg
     1741 ccttcggtta cctgaacatt caccgtgcac gtggtttcgc cgaaacgatt ccgtgccgtg
     1801 aaggtatacg tggtactttc ggtaggactc accgagcgcg tgccggttgc gtctacgtta
     1861 cccaacccgc tgatggacac ggtatcggct gcctgtgtcg cccagaagag cgtagccgat
     1921 tcgccctgca tgatgttcat gggctgtgcc gtacaggtct ggaaccgggc ttccggacgg
     1981 cggacggtga tggtgatggt ctcacttacc tcgttggtgt cgtttttcgc ggtcagacgg
     2041 tattccgtcg tatccgcggg agaaacggtg gaggttccgg cgtttgcatc tacgcgaccc
     2101 aggttggtaa tgttcacttc cgtggcgttc aatacgcgcc actcaatggt cgaagtctgg
     2161 cctgcgtcga tggcggccgg attagccgca aaacgtgcaa tctgcacctg gggtgcgctg
     2221 cgcgtgatga cactcacccg gtcgatgctc tgcgctcctt tgtcatcctt gacggtgacg
     2281 cggaacgcat agctctgccc ttcctccgcg gtgaatgtag cgacagccga agtcatgccc
     2341 gacagagtaa cggccgggcc ggcgacctgc gaccactgga aagtaatcgg gtcttcttcc
     2401 ggatcgaatg aaccggatgc atccaactgg attggtcctg ccggcacgcc aacctggtct
     2461 gcgccagcgt cggccaccgg gggatcgttg ccggtgcgaa cgcggcgggt gagcagttca
     2521 taccggatgc gtgggacgtc tgtcggaacg gcgtgatcca cgcccaggta gtctttcggg
     2581 cgaatgaagt tgccgaactg gaagccggcg accacacgac cggcgttgcc gcgagccagc
     2641 agcgtctcgt tcatgccgcc ctcaagcgtg aaggcgaaac ggtcgctgat cgggaatatg
     2701 aacctggcgg taccaccgac gcgatttccg tgtccgcggc tgttcagcca gccgaggtta
     2761 ccctcgacat aattctcgcc aaacaggccc agagtcgtac tcgcgccata ctggtccacc
     2821 gtgctcaggt aggtttcctc gaacacgttt cgggtgagag ccacacggtt gacgatcgcg
     2881 tcattcatga agcccttggc cgcgaaaaca ccagcccggc cgtggctgaa caggtaatcc
     2941 aaggtgaagg aagcctggcc cagagttcca ccagactgca tctcacgcag cgtgacgtgc
     3001 ttgaagctgg cgaacatgcc ggcctgcgca cgagtgtgaa tacgattaac gagaccgatg
     3061 tcgaactggc cttcctggcg atcgcggaaa tacatgtatt cgccctgggc ctgcaccgca
     3121 aaccggtctt taaacggcgc gaagtaacgg cccataccgg tgaaggtcag gtcgcggttg
     3181 tcatccgcac cgaaattcag ccgggaaatc gagaagcgcg gcatacgggc cttttcgatc
     3241 tcctccgccg tggcggtacg ggcaacctgc gtcgtttcgt cacgcgttag gggtttgggc
     3301 aacccggcga cttgctggcg caggtcgtcg tgttccttcc gcaacttctc cagatcggct
     3361 cgaacaccac cgagatcctt cagcatgctg acgatctcat ccaggcggtc cagccgtttc
     3421 aggatctcct cgcaacattc ctgctgcttt ttggcccaat ccggaatgac atcgatggct
     3481 tctttgaccc ctccggccgc aactcggcgc ccctggtcgt cgaagacatt tacttccacc
     3541 cggcggttca taaaacggcc ttccttggtg cggttgggca ctttcggaga ttcctctcct
     3601 tgagtggccg tttccacctg gccgtcactc gcgccatatt tcaccagaaa ccgcttcact
     3661 tcttctgcgc gggaagttcc taatttctgg ttgtaccggt ttccgcctat ccaatcggca
     3721 tttccgacga gcctgacttt gtaacctggg ttctgctgca acaactctgc taatcgcaac
     3781 aggctgggat atccgtcaga aagtatcgcc gaattgaatt caaaattgat ctcttcccaa
     3841 tcgtcctttg atgcgttcgt ttgcagaccc tgggcaaaaa cgagcgcaga caggctggtg
     3901 aagagcacaa gtagtttcag gtagcgctgg aacatgatcc gaactcctcc tcatcaactg
     3961 gtcgcaacta ctctatctgt actgcttgat gtactgcttt aaccaaagct catatactgt
     4021 tctaccaatt cacaacatac aatgtcgaaa atggaaaatc gacccccggc gaagcgcagc
     4081 acttcccaca tccgggaaat gtgcggaagc cccatccgga gcaaggatgg cacctttgca
     4141 tttttactga aagattagat gcggaattcg cgggatcatt tcaagatcat gagataatat
     4201 atgcaagcta aagcttgccg gatgggtaac cgctcgtttt gaacgggagg aaagtccggt
     4261 ctccacaggg cagcgtgccg gctaacggcc ggggggattc gctcaaagcg gatttcacgg
     4321 aaagtgccac agaaaatata ccgcctgcct cggcaggtaa gggtgaaaag gtgcggtaag
     4381 agcgcaccgc gggcggagtg atccgtccgg cagggtaaac cccacgcgga gcaagaccaa
     4441 ataggagagg aggagcggcc cgctccgcca gactctcggg taggtcgctt gagccgtccg
     4501 gtaacggccg gcccagagga atggttaccg cccgcaaggg tacagaaacc ggcttatagt
     4561 ccggcaagct catttgttcc ggccatcggg tcagccgcct cgcttcctg
//
LOCUS       DASTMK010000432         4959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_118041, whole genome shotgun sequence.
ACCESSION   DASTMK010000432 DASTMK010000000
VERSION     DASTMK010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118041"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFQ79_18495"
     CDS             complement(<1..634)
                     /locus_tag="VFQ79_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0141718.1"
                     /translation="MSAPLRKAVFPCAGLGTRFLPATKAQPKEMLTLVDKPLIQYGVE
                     EALGAQIPDIVVITSRGKSSIEDHFDFSPELEAALEEKGKAKRLAEVKAISDLINICF
                     VRQKRALGLGHAVNCARDVVGNEAFAVVLPDDIIMSHVPVLKQMKDVHEALGASVLAL
                     MEVPRERISAYGVVDAEPHPYPGADGRLFKLRGMVEKPKAEDAPSNLAIIG"
     gene            complement(755..4039)
                     /locus_tag="VFQ79_18500"
     CDS             complement(755..4039)
                     /locus_tag="VFQ79_18500"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141719.1"
                     /translation="MALKQFLAFLILLTLAAAAAWPQGGQGSIVGTVTDDSGAVIPKA
                     KLTAINERTAAERTVEADESGQYTIPNLPASRYTVTAEAEGLGPNELRNVTLTAGQER
                     AVNIVLQPAALAQEVSVSAGELVVIDTSSARVGVNVNEREVATLPLNGRQLSQLYLMA
                     PGAQSAGGGSYDNVRFSGRANQQNAIRFDGIEASSIVDSSPGNLNGESSTSFRLQSSL
                     ENVQEFRVESSNYPAEFGTGTGGQISVVTKSGSNEFHGSVFHYLRNDALDARNFFDRE
                     SASPLRLNQFGGSLGGPIRRDRLFFFASYEGLRQRAYQNLIEAVPSVAARRRAVPSIR
                     PLMDAYPLGVPTSNPDLDLAQIRAGAPIDENYGSIRLDYRISDATNLTARYFRDQGEL
                     TQPLNVTGNSARTSAVPQNALLNLQTVLHANAVNEIRLGMNGSKTRYNGVAPNVNGLD
                     LSAVSIDFAGTATIEGIGGQGVSGGAARIGGLVRSSSAQNGRGIPYTNYTLTFADQLS
                     WIKGNHSMKFGVEVRPIRMYTDRLGGTTYTYSNVNALLNNTPSQIQILGDVSAPNPLH
                     DGVAGNRFLKQEYYIGFAQDEWRIRPNLTMNYGLRYEYYSVLREDRGLFTFFDMNTGN
                     LDTNPDRPWYQSSKGNFGPRLAFSWSPERLNGNTVLRIGAGYYFGPGQTEDQVQPIDS
                     DRVTVTQTSNIAFPVNSQAIINSFDPNNLANFAPRAYAPGYRLPEKILSYTASIQQKL
                     PFETVLSVAYVGSQGRNLFLRSWTNLLVGVNMNPTTGAGIPVLQFGNKFSQLDYKTSG
                     GTDHYDSLQTTVNRRFHRGLTVGSQWTWAHSLGNAGGSNEAQTAQNPFNFEQDRGNNA
                     FDVRHSFNASVLYELPFGRGKTSRWARLAMADWEIGGIVNARTGLPIDVTLARNDIAY
                     RVNSTGRIVDQPIVSGGQILTTPVINNPWGGAFRGNRRPSVIAGVNPDLSGSGNDKRA
                     FLNPAAFTIPAPGEFGNLGRWALHGPGLAQFDLTLHKKFAIAETKALEFRAEVYNVLN
                     RANFANPVPRLSNALGVGGLQLQPGQPFSSGTAGGGFGLLTSTVTKDVGLGASRQMQL
                     SLRFTF"
     gene            4188..>4959
                     /locus_tag="VFQ79_18505"
     CDS             4188..>4959
                     /locus_tag="VFQ79_18505"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU0141720.1"
                     /translation="MLLVALAAVDILISRVAETHYRETLIRDLRSTGEMLALCPPGQA
                     TELARAAVLRLTIVDDAGRVRVDSDADPLHMESHRERPEIREALSGRHGTSIRRSPTL
                     GIDFLYVAVPYGKGALRLAMPLSDVSRQVNSLRLQTFKAALIAFFPAILVAGFFARRV
                     SRRLARIIEFSTKLAKGDLTAKLHSSSGDELGILATTLNETARNLEQTFGQLQSERKE
                     MEKLDRVRKDFVINVSHELRTPLASIQGYTETLLDGALH"
BASE COUNT          919 a         1463 c         1544 g         1033 t
ORIGIN      
        1 gcccgatgat cgccaggttg ctgggggcgt cttcggcttt cggcttttcc accatgccgc
       61 gcaatttgaa gaggcgcccg tcggcgccag gataggggtg gggttcggca tcgacgacgc
      121 cgtaagcgct gatgcgctcc cgggggactt ccattagggc cagcacggag gcgcccaaag
      181 cctcatggac gtctttcatc tgcttaagaa cgggaacgtg cgacatgatg atgtcatcgg
      241 gaagcaccac cgcgaaggct tcgttgccca caacgtcgcg ggcacagttc acggcgtggc
      301 cgaggccgag cgcacgcttt tgacggacga agcagatatt gatgagatcg ctgatcgctt
      361 tcacttccgc cagtcgcttt gccttgccct tctcctcgag cgctgcctcc agttcggggc
      421 tgaaatcgaa gtggtcttcg atggagcttt tgccccggct cgtaatcacc acgatatcgg
      481 gaatctgcgc gcccagcgct tcttccaccc catactggat aagcggctta tccaccaggg
      541 tgagcatctc tttcggctgc gctttggtgg cgggcaggaa gcgggtgccg agcccggcgc
      601 aggggaagac ggctttgcgt agcggggcag acatagaaat ccatcgtatc tccaatgcgc
      661 gggtggctgt ggtgaagagc cgtcaaagag aagtggcctt ccgaagtctt gaggacttgg
      721 gaaggccaca tggcgtaccg gcgacattcc ggatttagaa cgtgaagcga agcgaaagct
      781 gcatctggcg gctcgcgccc agcccgacat ccttagtaac cgtagaggtg agaagcccga
      841 atccgccacc cgccgtccct gagctaaagg gctggcccgg ctgtaactga aggccgccga
      901 cgccaagcgc attgctcagt cgcggcacgg gattggcgaa attcgcacgg tttagcacgt
      961 tgtacacttc agcccggaac tccagggcct ttgtttcggc aattgcgaat ttcttgtgca
     1021 gagttaggtc aaactgggcg aggccagggc cgtgcagcgc ccatcggccc aaattaccga
     1081 attcgccagg agccggaatg gtgaacgcgg cggggttaag gaacgctctc ttgtcatttc
     1141 cagagccgct caggtccggg ttgacgccgg cgatgacgct gggcctccgg ttgccgcgga
     1201 aggcgcctcc ccaagggtta ttaatcacgg gagtcgtcaa gatttggcca ccgctcacaa
     1261 tgggctggtc gacgatgcgg cccgtcgagt tcacccggta ggcgatgtca ttccgggcca
     1321 gggtgacatc aatcggaagg ccggtccggg cattcacaat gcctccgatc tcccaatctg
     1381 ccatggccag acgtgcccac ctggacgtct ttccgcgtcc aaatggcaac tcatagagaa
     1441 ccgaagcgtt aaagctatgg cgcacatcaa aagcgttgtt gccgcgatcc tgttcgaaat
     1501 tgaaagggtt ctgcgctgtt tgcgcctcgt ttgagccgcc cgcattgccc agcgagtggg
     1561 cccaggtcca ctgcgatccc accgtcagac ctctgtggaa gcggcggttc accgtcgtct
     1621 gcagggagtc atagtggtcc gttccaccgc tggttttgta gtccaactgg gagaacttgt
     1681 tgccgaactg gagcacggga atgcccgctc ccgtcgtggg attcatgttc acccccacca
     1741 gtagattggt ccaggaacgc aggaacaggt tgcgtccctg gctgccgaca taggcgacgc
     1801 tcaaaaccgt ctcaaacggc aacttctgct ggatggaggc agtataggag aggatcttct
     1861 ccggcagacg atatccgggg gcataagcgc gcggagcgaa gttcgcgagg ttgtttgggt
     1921 caaatgaatt gatgattgcc tggctgttta ccgggaaggc gatgtttgag gtctgggtga
     1981 cggtcacccg gtcgctgtcg attggctgaa cctggtcctc ggtctgcccg ggtccaaagt
     2041 agtagcccgc tccgatgcgc agcaccgtat ttccgttcag ccgttctggc gaccaggaga
     2101 atgccaatcg gggtccgaag tttccctttg aactttgata ccacggccgg tcggggttcg
     2161 tatcgaggtt ccccgtattc atgtcgaaga aagtgaatag cccccggtcc tcgcggagca
     2221 ccgaatagta ttcatagcgc aggccatagt tcatggtcag attcggccgt attcgccatt
     2281 cgtcctgcgc gaacccgatg tagtattcct gcttcagaaa ccggttgccg gcaacgccat
     2341 cgtgcaacgg gttgggcgcg ctcacatcgc caagaatctg gatctgcgac ggcgtgttgt
     2401 tcagcagggc attcacgttg gaatatgtgt aggtcgttcc gcccagacga tccgtgtaca
     2461 tgcggatcgg gcgcacctcg acgccgaatt tcatcgagtg atttcctttg atccagctca
     2521 actggtccgc gaaggtcagg gtgtagttgg tatatgggat gccgcgtccg ttctgagcgc
     2581 tcgaagaacg aaccagaccg ccgatccgcg ctgcgccgcc ggagacgccc tgcccgccaa
     2641 ttccctcgat cgtggccgtt ccggcgaagt cgatagaaac cgcagagagg tcgaggccgt
     2701 tcacgttggg agccacgccg ttgtatcggg tctttgagcc gttcatgccg aggcgaatct
     2761 cattgacggc gttagcgtga agcacggtct gcaggttcag caacgcattc tgtggcacgg
     2821 cggaagtccg tgccgagttg cccgtcacgt tgagcggttg ggtaagttca ccctgatcgc
     2881 ggaaataccg ggctgtcaga ttggtcgcgt cgctaatccg atagtccaaa cggatactcc
     2941 cgtagttctc gtcgataggg gctcctgctc ggatctgcgc caggtccagg tcgggattgg
     3001 aggtgggaac acccagcggg taggcgtcca tgagcgggcg gatggaggga acggctcgcc
     3061 gtcgagcggc cacgctggga accgcttcga tgagattctg gtaggcgcgt tgccgcaatc
     3121 cctcgtagct ggcgaagaag aacagtctgt cgcgccggat cgggccgccg agtgagccgc
     3181 cgaactgatt gaggcgcagc ggcgacgccg attcgcggtc aaagaagttc cgcgcgtcca
     3241 atgcgtcgtt acgcagatag tggaacacgc tgccgtggaa ctcgttggaa ccggatttgg
     3301 tgacgacgct gatttgtccg cctgtgccgg tgccgaactc ggccgggtag ttgctggatt
     3361 ccaccctgaa ctcctgaacg ttttccaggc tggattgcag gcggaaggat gtggagctct
     3421 cgccgttcaa gttgccgggt gaggagtcca caatggagga ggcctcaatc ccatcaaacc
     3481 gaatggcgtt ctgctgattc gcgcgcccgc tgaagcggac gttgtcgtag cttcccccgc
     3541 cggcgctctg tgcgcccggc gccatcagat agagttggga aagttgccgc ccgttcagcg
     3601 gcagcgtggc aacctctctc tcattgacgt tcacgccgac acgggctgag cttgtgtcga
     3661 tcacgacaag ttcccctgcg gagacgctaa cttcctgcgc gagagcggcc ggctggagga
     3721 cgatgttaac cgccctctcc tggcccgccg taagcgtgac attcctgagt tcattgggcc
     3781 ccaatccttc cgcttcggcg gtcacggtat atcgtgaggc ggggaggttg gggatggtgt
     3841 attggccgct ttcgtcggcc tcgacggtcc gctcggcggc ggttctctcg ttaatcgctg
     3901 tcagcttggc cttcggaatc accgcgcccg agtcgtcggt gacggtgcct acaatactcc
     3961 cctggccgcc ctgcggccat gcggcggccg cggccagcgt caacaggatg aggaacgcaa
     4021 gaaattgttt tagtgccatc gccaccaatc tagcggtcgc ctgttacaga ttgatgaacg
     4081 aaccgtgaat gttcggcgag ccgggattgc ctggactgcc cgggccttat gatgggagca
     4141 aggtgacctc gcggatcttc gctaaactcg tctttgctgg cgcttgtatg ctcctcgtgg
     4201 cattggctgc cgtcgacatc ctgatatcgc gcgtcgcgga gacgcactac cgcgagaccc
     4261 tgattcgcga tctccgttcc acgggggaga tgcttgcgct ttgccctccc ggccaggcta
     4321 cggaactggc gagagcggcc gttcttcggc tcaccatcgt cgacgacgcc ggccgggtgc
     4381 gcgtagattc agacgccgac ccgctgcaca tggaaagcca ccgcgagcgt ccggaaatcc
     4441 gggaggcgct gtctggccgc cacggtacca gcatccgccg cagcccgaca ctgggcattg
     4501 atttcctcta tgtcgccgtc ccgtacggca agggcgcgct gcggctggcg atgcccctct
     4561 ccgacgtcag ccggcaagtg aattctctcc ggcttcagac cttcaaggct gcgctgatcg
     4621 ccttttttcc cgcaatcctg gtcgcaggtt tcttcgccag gcgggtctcc cggcggctgg
     4681 cccgcattat cgagttttcc accaaactcg cgaagggaga cctcacggcg aagcttcatt
     4741 caagcagtgg ggacgaactc ggcatcctcg ccacgacgct caatgagact gcccggaacc
     4801 tggagcagac gttcggacaa ttgcagtccg agcgcaagga aatggagaag ctcgaccgtg
     4861 tacggaagga ctttgttatc aacgtctctc acgagttgcg tacgcccctc gcctctatcc
     4921 agggctacac cgagaccttg ctcgacgggg ccctgcatg
//
LOCUS       DASTMK010000433        20561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_310378, whole genome shotgun sequence.
ACCESSION   DASTMK010000433 DASTMK010000000
VERSION     DASTMK010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20561
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310378"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFQ79_18510"
     CDS             complement(<1..426)
                     /locus_tag="VFQ79_18510"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU0141721.1"
                     /translation="MQNSGIRPSHFGLEEHGIRNANTVFWNLGTAQLIEKAIQRREGL
                     LASGGALVVRTGDHTGRSPKDKFVVRDETTESSVNWGPVNQPFTPQAFDWIYGKLLAY
                     LQARDLYVQDCFAGSDPEFQLPIRVITERAWHNLFARQLF"
     gene            624..1619
                     /locus_tag="VFQ79_18515"
     CDS             624..1619
                     /locus_tag="VFQ79_18515"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU0141722.1"
                     /translation="MDNLTHTLTGIALSRAGLNRWCPHAAAVLVLSSNAPDIDAVAGF
                     WGATTYLHYHRHLTHALVSIPVLALLCAASVALLFRKSIKLRRDAFRLTATAAVGVAL
                     HQGMDFTNIYGIRLLLPFSSEWLRLDIINIVDLWLWVVLFLAATAPLLSRLVNSEIGA
                     KGGSGRGLAVLALSFLVLYSGARAVLHARAVETLNSRLYAGQLPRRTAAFPSFWNPFA
                     WRGLVETDSFYSLHELDLLEQFDPTAGRTLYKIARDEVTEAASRTEAFRVFLAFSPYT
                     FWRIEKDTEEGLLRVEAMDLRFGAPPEPRFVATAIVDTAGRVRSSQFGFGPLRPR"
     gene            complement(1625..2890)
                     /locus_tag="VFQ79_18520"
     CDS             complement(1625..2890)
                     /locus_tag="VFQ79_18520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0141723.1"
                     /translation="MKIPKMRWVIAAMLCMATMINYADRLTLAVVSVEVRNEFGMTEQ
                     DYSYILALFMLAYAIMYAGSGYIVDRLGTRRGFAVFIFSWSLAQMMHALTRGKTSLGA
                     ARFALGLTEPGNWPAAAKAIAEWFPASQRALGVGIFNAGSSLGSAIAPPMVAVITLHY
                     GWRASFIFTASLGFVWLSFWLLLYNPPHRNRWLRRSEYEAMKDHIRPPEETLPADSKT
                     LNWRRVISMRQCYTLILTRFFTDPVIYFVIFWLPEYLRRERGFDLALVGKYAWVPFIF
                     GDLGYVLGGWLSGRLIKGGWSLPRARKFVMLLGASVMPAAMLAPFVSEAWMAIGAICF
                     VTFGHAFWVANLQTLPTDLFRATEVGTATGFSGMGGAIGGVLAQLGTGYIVTHFSYAP
                     IFLLAGVFHPLSAALTYWLLPDRYFKQRA"
     gene            2996..3481
                     /locus_tag="VFQ79_18525"
     CDS             2996..3481
                     /locus_tag="VFQ79_18525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEU0141724.1"
                     /translation="MALSIDDTISVLNNLIETCKDGEEGFRSAAEGTDDSQLRALFNE
                     YAMQRGGFAAELQRQVAGLGGSPERSGSLAGAAHRGWMDIKSAVMGRDNKAIVNEAER
                     GEDSAVEQYTDALARDLPSDVRSIVERQARMVREAHDRIRDLKSQYEMGKPRTRTGGV
                     Y"
     gene            complement(3539..4786)
                     /locus_tag="VFQ79_18530"
     CDS             complement(3539..4786)
                     /locus_tag="VFQ79_18530"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU0141725.1"
                     /translation="MSRRVVITGLGAVSPNGVGRDNFWAATSHGVSGIRKITRFDASP
                     YAVQVAGEVDFPEKNYVDPKDRPHVSRVVPLSRAAVEEALSDAGLDAGRMSRDELRGI
                     GVILGSGGGSGEFSEEQYRLYYEGHHKQCSVYVIPSSTMGTLASEISMRFGFRGLSHV
                     VTTGCTSSTDALGYAYRNIQAGVLDTIVAGGADAPIAPLIFRGFLLMRIMASKWNEEP
                     HRASRPFSRDRDGFVLAEGAWFFVLEELERARARGAKIYAELVGYGSTCEAYHRVRLE
                     ECGEEPARAMGLAMEEAGIAPTAVQYINYHGTSTDLNDRIETRAVRLAFHSHAYRLAG
                     SSLKSLIGHPQGACGAAGVAATILAMQSGVAPPTINLDAPDPECDLDYIPDIGRQMQI
                     EYAVANCIAFGSKNSALVLRRMD"
     gene            complement(4839..5336)
                     /locus_tag="VFQ79_18535"
     CDS             complement(4839..5336)
                     /locus_tag="VFQ79_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141726.1"
                     /translation="MPARSVVPAGRSASIALVEVTMEHDPSKSVTALAILSSEEDRLS
                     LHRLFGNSNWILHTVDTFEEAVEVIRRTPMPVVIAEDRLGRRTWRDLLYALDGVEKPP
                     VIIVTSRMANESLWGEVLNFGGYDLLMEPFERNEVYRVISLAWLHWKHGRTQPDIRTH
                     AVPAG"
     gene            5498..6826
                     /locus_tag="VFQ79_18540"
     CDS             5498..6826
                     /locus_tag="VFQ79_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141727.1"
                     /translation="MQTDSTIPSRRSFLRKGSIAGAGVSAFTIIKPELVRGAGNEKLK
                     AGVVGCGGRGTQAVVDLLTGSQNVDVVAMADAFEDKLEGSLRRLRAHKESGKWGDRLK
                     VDPEHRFVGFDAFKKLVNSGVDIVMLATPPGYRPEHFEAAVNAKKHVFCEKPFGTDPV
                     GVRRFMTAARKSEEMKLTVVTGAQRRFARDYQETVEKINSGAIGDIVATYAYWVGTPV
                     VQQKAGRNPKWGDMEWQHRNWYSYVWICGDQIVEQHLHNIDVCNWIMGTHPVKVVATG
                     GAIWRPKTEVYGNIYDHISADFEYPNGVRMSSYCRQFPRDRNSEIHTNISELIVGTKG
                     RSNAHDLGTGSGQDVRTSFRDPLENPYVREHTAMVASIRGDGPYINHAMAVAESTMTC
                     IMGRESAYSGLEITWDMIMNSKQDLQPKQFGYDLKMEVPPLPVPGEYKFV"
     gene            complement(6865..7485)
                     /locus_tag="VFQ79_18545"
     CDS             complement(6865..7485)
                     /locus_tag="VFQ79_18545"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0141728.1"
                     /translation="MLPRNHPEVSAAAVENGAEKAAWRTVPNLLTLLRLALIVPFAWF
                     AILGRDMEALGVFALAGLTDAFDGYIARRFGQRSKFGRLADPVADKILTSTAFVVLSA
                     FRGQFVSLPLWLMAAVVLRDVLILIGCFVVYLLTRSTAFRPTISGKINTILEIFTIVC
                     FLASASLPAISQALGPLYLVLLVSIVISAADYVLLGSRMIRNSPRV"
     gene            7567..8484
                     /locus_tag="VFQ79_18550"
     CDS             7567..8484
                     /locus_tag="VFQ79_18550"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0141729.1"
                     /translation="MRIGITYLYAIFRYGYPHSVADALRSIPDIRKLGFRFLEMEGLG
                     RPFLRALYKDRNTLRQVVEDNGVHVHNFCVVDPDMVSLHPGKQKAALDGFKKGAEIAA
                     MLGTETLHLASYAPPVRYLDQKPYDLGAEEGYSFAGGSRIRIPKDFDWNSVWIALVDS
                     CRQCADIAANYGKTVIMEPRVGEVICSVDSQLRLIEDVARPNFKANFDTAHFSAQREN
                     VVLALEKLRNRFANIHIADNNPKDTGHLAIGDGVIDWEEFFVVLKRMNYTGYLGLDLG
                     TSRHLAADYRKSLDRIQSIASKLKIKIEV"
     gene            complement(8497..8733)
                     /locus_tag="VFQ79_18555"
     CDS             complement(8497..8733)
                     /locus_tag="VFQ79_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141730.1"
                     /translation="MRADVSDPGQVTVNTEFLGVVPFITPSMVKMKLAGAEVSTRGSM
                     LPNTTASAVSQAVRNGPCGPAFATAQAKMIKNAE"
     gene            8825..9589
                     /locus_tag="VFQ79_18560"
     CDS             8825..9589
                     /locus_tag="VFQ79_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU0141731.1"
                     /translation="MFASGLALPPNNRSGRDQIPTGTCHEVVWAVLKDSAAYIDLSPA
                     CLTAMETLLREDPPETAAALRDFSESIQRFAQSASVNLRALALASGIRRQDVQSLAQF
                     AAQASLLAKSQVIHPAAAAVAAWRDPDPAGLAALGTISQTAGADTLSRSAAQALMMIH
                     TKDTVPHLAKLLSSTDPELRGAAIRGLSFFVKGVPILSAENLRSMAYFAEVENPEYFD
                     ETISPYVSIIPVSPAKEAEYVNAWQAWWRRMATRFN"
     gene            complement(9686..9901)
                     /locus_tag="VFQ79_18565"
     CDS             complement(9686..9901)
                     /locus_tag="VFQ79_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141732.1"
                     /translation="MSPSFLARNQHSLIHAGLINGLTDSPEGIEPTLPTLTLIKEVPD
                     RLFDQFIPAVIPAAGEFLLDLPSQIGW"
     gene            9889..10446
                     /locus_tag="VFQ79_18570"
     CDS             9889..10446
                     /locus_tag="VFQ79_18570"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0141733.1"
                     /translation="MTGSPLVVIDACVLANFSLCDTLLRLAEPPRLYEPKWSEEIIRE
                     TTRTLESKLGWPSPLTASLQAELRAHFGETWISGHEALIPQMTNDEKDRHVLAAADHG
                     QASIIVTFNLRHFRREHLEPWGICALHPQSFLIETFRQEESLGMTKLEQQAADRRRSL
                     RELLHILSATVPDFVAVVSAAFSRE"
     gene            complement(10462..12084)
                     /locus_tag="VFQ79_18575"
     CDS             complement(10462..12084)
                     /locus_tag="VFQ79_18575"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0141734.1"
                     /translation="MPSARVIVLRFGLVLAFCLALAFAQFRETLRPSFAATLELVTRD
                     ANSGRTVPARVYLFKDGRPFRFSPVDSLLALRVDLFYREVLWRRAADPATLEITANGE
                     SHFLLLRGTAAFDLPAGRYRAEAYRGLFYKPAVQEFELRAGGKQRIELDLAPLTEHEQ
                     WLSADDHIHLVRHREDDPVFLGWIDGEDLSVGNFLQLQRQQDAAMQYGFGEAAEARME
                     GRSIRSGMEARSHFYGHVNLLGPREILRPLSIGEVYANSREAYPFPTVLFARARSLGA
                     TVGYAHFDGSQKNSTLLMDLALGSIDFIEVFQFGVLKTESWYELLNAGFRVTGIAGSD
                     FPVPLNNRKPWPRSLPLLGPERTLVKAAPKPSAYEAWAAGVRIGRVVVSNGPLLDLTV
                     DGSAPGAVINWSGVQKPVEGEAVARYHRPLEKLEIIVNGRTVASIAGEGRHEVRLPFR
                     FNLEESSWVAARATSASQEGEPQIQAHANPVYLLREGRHVSVNGARRALLLRWQQQAG
                     YYRSPDLEFSERRHREELLSNVEKTLAVLGSE"
     gene            complement(12114..13226)
                     /locus_tag="VFQ79_18580"
     CDS             complement(12114..13226)
                     /locus_tag="VFQ79_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141735.1"
                     /translation="MNRRLFCSMLPAVPSALAAADSTSSVDYPRIAARILEPLKLRRG
                     ERVLARFDPEYFAELLPLLRQNVRSAGAVWLEPLPAAAPVSATTAGRVDVFIRLPLGP
                     SARALAPAEERHLVDWVDKGGTRRELHFHWSDGTRHPDGLPTAHPPEFDALYMRALNI
                     DHQALRAVQDRAIRILRMGTVRIQSPNGTDVMFRIAKRPFNIQDGDASPERAQAAQVR
                     VDRHIELPAGVVRVAPVEDSAFGSIGLAEARFDDTVAKGVVILLDNGRVTGVRAKEGL
                     DAVKAALAAGGDAAMRFREFGLGCNPALTVPEGSGLIPYYGYGAGVVRLSLGDNEEIG
                     GKVRGGFVRWFFLPDTTLHVDFRYLVRNGKLTVPET"
     gene            complement(13233..14960)
                     /gene="ilvD"
                     /locus_tag="VFQ79_18585"
     CDS             complement(13233..14960)
                     /gene="ilvD"
                     /locus_tag="VFQ79_18585"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0141736.1"
                     /translation="MNEGIENIRRRGSRILTEGVHMTPAASLMRAEGVIRSMDDLKRP
                     FVTIINSYTTQIPGHAHLDRIGAIVFEELKSLGYNVWYTNVGAAICDGIAMGHFGMKY
                     SLPSRELIADQIESIIGAHPCDAWIGIGNCDKIVPAMYNAMVRINIPAVYVSGGPMLA
                     GTGNSDLISVFEGVGKYTAGKMTEQGLRGLAERACPGCGSCSGMFTANSMNCLGEVIG
                     LALPGNGTITAEQWVDRAEGITELNPERIELTRRAARTLKSCLDNNVRPLDIVTRQAL
                     DNAFVLDMAMGGSTNTVLHALALAHSAGIDYRLDRLNEISDTTPNICKVSPSRPEMHV
                     EDVHRAGGIGTILKELACHAKTVLNLDTAGVAGRLGEYVTASPDADGDVIRRVSNAFS
                     ATGGLTVLFGNLAPHGAVVKTAGVDAAMMEYEGPARVYESQEAALKGILEREVREGEV
                     VVIRYEGPRGGPGMQEMLSPTAALVGAGTRAALITDGRFSGGTRGLCIGHVAPEAAAG
                     GPIAAVRDGDVIYVNTTTRRIELKVDAGEIERRLAALPPFEPKVKRGWLARYSHHVTS
                     ADTGAVMAI"
     gene            15043..16524
                     /locus_tag="VFQ79_18590"
     CDS             15043..16524
                     /locus_tag="VFQ79_18590"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEU0141737.1"
                     /translation="MPVVSRREWLASLFACPALSIAAPDPEAWIPLFDGASLDGWTAG
                     ENRDSFRVEGGTIVAAGPRSHLFYTGGIREASFRNFECRADVLPHPGANSGIYFHTRF
                     QKQGWPTAGFEVQINNSYGGEGRYRERRKTGSLYGVRNVYKAFARDGEWAQLHITVHS
                     KQVQVRLNDLLLVDYVEPDPPVRADKTMDRVLNRGTFALQCHDPGSRTHFRNIAVKPL
                     PDDVRASAAAPEVDDVYRQILDLSARNFPVVDYHTHLKGGLTIDEMLRESRRLGIQYG
                     VAVNCGVSFPIHNDAGLNDFLETMKGQPVFIALQAEGREWVNLVSSEMVARFDYVFTD
                     SMTYTDDAGKRMRLWIKEEVGDIRDPQAFMETYVDRTVGILSREPIDIYANPTFLPDV
                     ITSQYDVLWTEERMRRVVEAAAANGVAMEINSRYRLPSPAFIKMAKAAGVKFSFGTNN
                     DAKYLGRCEYGLRMVKECGLDWHDFFIPKEDGNKPVQVRGLKG"
     gene            16549..17388
                     /locus_tag="VFQ79_18595"
     CDS             16549..17388
                     /locus_tag="VFQ79_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0141738.1"
                     /translation="MSDLSRRDFGRLAFLGAALTTNHVALSGANGNIGIGVQLYSVRK
                     DAAADLRGVLAAIGRMGYQAVEFAGYYDHDAKAVRKMLDDAGLKCCGSHIGLPALRGD
                     ELSKTVEFNRIIGNKYLVVPGLPKENRASRQAWIETASVFNEISAKVKPHGMRVGYHN
                     HTIEFQPLDGEKPWDTFFGRTNKDVIMQLDIGHAQRAGNDPIDVMKKYPGRAVTVHVK
                     EFSPSDKNALIGEGQINWKGFFQAAQTVGGTEWYIIEEESGAYPGLEGIEKSIKNLKK
                     LLS"
     gene            complement(17398..19491)
                     /locus_tag="VFQ79_18600"
     CDS             complement(17398..19491)
                     /locus_tag="VFQ79_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141739.1"
                     /translation="MPHLRVLLPFLLGFAMISPPLKAQGQNARPDWIAGAVSALEKEL
                     VDKYGPTQRVRVERGLKQVAGFWRADDGDRNAFEEFVRTNFAGDQKALDTVFSRYETL
                     FEQLDGHMLEIQIAFRLQVDLDRGGPIMPYDEIFAAYDPSAHTKEDFFKNKLAFVVLL
                     NFPLTTLEERLARGEQWSRREWAEARLAQRFSRRVPPEVNLAIAEAGAQAEQYIAQYN
                     IWMNHLLDEKGQRLFPAKMRLLSHWNLRDQIKADYTEKDGLPRQRMIARVMEHIVRQT
                     IPQAVVNNPTVDWNLSWNSVSRSEVKDYSEVPRPARQASNEREPDTRYAMLLGTFRAL
                     RQIDPYSPAAPTHMARIFDEERELPEERVQAMFEQILTSPLIPRVAKLIEQRLGRPLE
                     PFDIWYAGFKPRGKYSEAELDEITRKRYPTPEAYEKDMPRMFRDLGFSAERADYLASN
                     IVVQPARGSGHAWGAQLRTAPARLRTRVGADGMDYKGYNIAVHEMGHNVEQTFSLKNI
                     DHWLLNGVPNTAFTEALAFVFQARDLELIGLKDTDESAEALKILNDFWATYEIAGVAL
                     VDMAVWHWMYDQPDATPEQLREAVIEIATDVWNRHYAPVFRHKDVVLLGIYSHMIERY
                     LYLPDYPIGHLIAHQLEEHMAKAGKIGPEVERVSKQGRIAPDLWMKGATGAPVGAEAL
                     IEAATRALARIAPSN"
     gene            19590..>20561
                     /locus_tag="VFQ79_18605"
     CDS             19590..>20561
                     /locus_tag="VFQ79_18605"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEU0141740.1"
                     /translation="MRILVLNGGSSTIKASLYDVEGGTMSGAAQRPIWRVHGGWGNSN
                     GTARIHAEGPGGVEDMELRIGAPGEVIEPALGMLLKGKAQVLRAWTEISTVGHRIVHG
                     GSRLRESALISEEVKSGISEAAEFAPEHNWFQLETIHTVERLLGPGVPQFAVFDTAFH
                     ATLPERTFVYGGPYEWLDRGIRRYGFHGISHQYVFGRAAEILGHDASGLKLVSCHLGN
                     GCSLAAIDGGKSVDTTMGFTPLDGLIMGTRSGSVDPGILIYLMQRGGYTADQLNRTLN
                     KESGLKGLSGISGDMREIMAAVEVGNRRAGLAFDAYIQRLCREIGGMV"
BASE COUNT         4358 a         6146 c         6089 g         3968 t
ORIGIN      
        1 gaaaagctgc cgggcaaaga ggttatgcca ggcacgttct gtaatcacgc ggataggcaa
       61 ctgaaattcc gggtctgagc ccgcgaaaca atcctgtacg taaagatcgc gcgcctgcag
      121 ataagcaagt aattttccgt agatccagtc gaacgcctgc ggtgtaaacg gctgattgac
      181 cgggccccag ttgaccgacg attccgtggt ctcgtcccgc accacgaatt tgtccttcgg
      241 tgaacgtccg gtgtgatcgc ccgttcgaac aacgagagcg ccaccgctgg ccagcaaacc
      301 ttcacggcgc tggatggcct tctcgatcaa ttgtgcggtg cccaggttcc agaacacggt
      361 attcgcattt cgaatcccat gctcctctaa accaaaatga ctcggacgga ttcccgaatt
      421 ctgcatttta gattttctcc tctgatctta tgacaaagat atgtagcaac ttgtcttcca
      481 ttttcaagca cttaagtttt catcgtgatt aacgctggat taaggtcata tagcccagta
      541 tcgtattaag acggcaaatc aatgtggatg aatacaccca atttgctagg attcttacag
      601 cgtccgccgg cgctccgcct tccatggaca atctcacaca caccctcacc ggcatcgctc
      661 tcagccgcgc tggcttgaac cgctggtgcc cgcacgccgc agccgtcctg gtattaagct
      721 caaacgcgcc cgatatcgat gcagtcgcag gtttttgggg agccacgacg tacctccatt
      781 accaccgcca tctcacacac gcgctggtca gcatcccggt gctcgccctg ctatgcgccg
      841 caagcgtggc tttattgttc cgcaagagca ttaaactgcg tcgtgatgca ttccggctga
      901 cggcaaccgc tgcagtgggc gtcgcccttc accagggaat ggatttcacc aacatctatg
      961 gcatccgcct gctgctgccg ttctcgtcgg aatggctgcg gctggatatc atcaacatcg
     1021 tcgacctgtg gctatgggtg gtgctgtttc tggctgccac cgctcctctg ttatcgcggc
     1081 tggtcaattc agaaatcggc gcgaagggag gcagcgggcg agggctcgcg gtgcttgcgc
     1141 tttcgtttct ggtgctttac agcggagcac gcgctgtgtt gcacgcccgc gcagtggaaa
     1201 ctttgaactc ccgcttgtat gccggacaac tcccccggcg cacggccgca tttccgagct
     1261 tctggaatcc cttcgcctgg agaggactgg tagagaccga cagcttctac tcccttcacg
     1321 agttggatct gctggagcag tttgacccta ccgccggacg cactctgtat aaaattgcgc
     1381 gcgacgaggt aacagaggcg gcgagccgta cggaagcatt ccgggttttc ctcgcgtttt
     1441 cgccgtatac gttctggaga atcgaaaaag ataccgaaga aggactgctg cgcgtggaag
     1501 ctatggatct tcgcttcggc gctccgccgg agccccggtt cgtggctaca gccattgtgg
     1561 acacggctgg ccgcgttcgc agttcccaat tcggcttcgg tcctttgcgg ccccggtgag
     1621 ggggctatgc acgctgcttg aaataccggt cgggaagcag ccagtaggtc aacgcggcgg
     1681 atagcggatg aaagacgcct gccagaagga agatgggcgc ataggagaaa tgggtcacga
     1741 tgtagcccgt tcccagttgg gccagcacac cgccaatggc accgcccatg ccggaaaatc
     1801 ctgtcgccgt tcccacctcc gtcgcgcgga acaggtcggt tggtaaagtc tgcaggttcg
     1861 ccacccagaa agcgtgaccg aatgtgacaa agcagatcgc gccaatcgcc atccaggcct
     1921 ccgaaacgaa cggcgcgagc atggccgcag gcatcacaga ggctcccagc agcatgacaa
     1981 actttctggc gcgcgggaga ctccatccgc ctttgatcag cctgccagaa agccagccgc
     2041 ccaggacgta gcccaggtct ccgaaaataa aaggaaccca ggcatatttg cccaccaggg
     2101 caagatcgaa cccgcgttcc cggcgcagat actcgggaag ccagaagatc acgaaataga
     2161 tcaccgggtc cgtaaagaac cgcgtcagga tgagcgtata acactgcctc atgctgatga
     2221 ctcgccgcca attgagagtt ttcgaatccg cgggaagggt ctcttcgggc ggccggatgt
     2281 gatctttcat cgcctcgtac tcgctccggc ggagccagcg gttccggtgc ggaggattgt
     2341 acagcagcag ccaaaaagac agccacacga acccgagcga cgcggtgaaa atgaacgatg
     2401 cccgccagcc atagtgaagc gttatcacgg ccaccatggg cggcgcgatg gcggatccca
     2461 gcgacgagcc tgcgttgaaa ataccgactc ccagcgcacg ctgcgacgcc gggaaccact
     2521 cggcgatggc cttcgccgcc gcgggccaat tcccgggctc ggtcagcccg agcgcgaaac
     2581 gggctgctcc aagggacgtc ttaccgcgcg taagcgcgtg catcatttgc gccagggacc
     2641 aactgaagat aaagacggca aaaccacgcc gggttccaag ccggtccacg atatagccgg
     2701 agcccgcgta catgatcgcg taagccagca taaagagagc aaggatgtag gagtagtcct
     2761 gctccgtcat cccaaactcg ttgcggacct caacggagac gaccgccaac gtgagccggt
     2821 cggcataatt gatcatcgtc gccatgcaca gcatggcagc aatcacccac cgcatttttg
     2881 ggattttcat ggacgaacca ttacaacgta tcgcagcgcc gatatgcaaa gcggccataa
     2941 aggcgaaaca agtgtgagcc aaccgagtcg aactttgcgg agggcgcttt cggttatggc
     3001 attaagcatt gacgacacca tctctgtttt gaacaacctg attgaaacct gcaaggacgg
     3061 agaggagggt ttccgtagcg cggcggaggg gactgacgat tctcaacttc gcgcgttgtt
     3121 caatgaatat gcgatgcaac gtggcgggtt cgcggcggag ttgcagaggc aggtagccgg
     3181 gctcggagga tctcccgagc gttccggcag cctcgcggga gccgctcacc gcggctggat
     3241 ggacatcaag tccgcggtca tgggacggga caataaggcg attgtgaacg aagcggagcg
     3301 gggcgaagat tccgcggtag aacagtacac cgatgcactc gcgcgcgacc tgcccagcga
     3361 cgtgcgatct atcgtggagc ggcaggcccg tatggtaaga gaggcccacg accgtatccg
     3421 cgatctgaag tcgcaatacg aaatgggaaa accccggacc cggacgggcg gtgtttattg
     3481 atgagcagga aaatcctccg gcggcggaac ccgctcctgt agcaacaggg gcgggttatc
     3541 agtccatccg cctcaacacc agagcggaat ttttactccc gaaagcgatg cagttggcca
     3601 ccgcatattc aatctgcatc tggcgcccga tatcgggaat ataatccagg tcgcattccg
     3661 gatccggcgc atccagatta atcgtagggg gcgccactcc gctttgcatc gcgagaatgg
     3721 tcgccgctac tccagcggcg ccacaagccc cctgcgggtg gccgataagg cttttcaacg
     3781 acgagcccgc cagccggtaa gcgtgggaat ggaatgcgag ccggacagcc cgtgtttcaa
     3841 tccggtcatt caaatcggtc gacgtcccat ggtagttgat gtattgaacc gcggtgggcg
     3901 cgatgccggc ctcctccatc gctaaaccca tggcccgcgc cggctcttcg ccgcactctt
     3961 ccagccgtac gcggtggtag gcctcacagg tggaaccgta tccaacgagc tcggcgtaga
     4021 ttttcgcacc cctggcgcgt gcgcgttcca actcctcgag aacgaaaaac catgcgcctt
     4081 cagccagcac aaacccgtcg cgatcgcgcg aaaacgggcg ggaggcgcga tgtggttcct
     4141 cattccactt gctggccatg atcctcatca aaagaaatcc ccggaagatc aggggtgcga
     4201 tcggtgcatc cgcccctccc gcgacaatcg tgtccagcac gccagcctgg atgttgcggt
     4261 aagcgtaacc gagcgcgtcg gtggaagaag tacagccggt agtaaccaca tggctcaagc
     4321 cgcgaaagcc gaagcgcatg gagatctcgc tggccagcgt tcccatcgtg gagctcggga
     4381 tcacgtacac actgcactgt ttgtggtggc cctcgtaata gaggcggtac tgttcttcgg
     4441 agaactctcc gctgccgccg ccggaaccga gaataacgcc gataccgcgc aactcgtccc
     4501 tgctcatccg tccggcatcg aggccggcat ccgacagcgc ctcctccacg gcggcgcggc
     4561 tgagcggcac gacccgggaa acatggggac ggtccttggg atcgacgtag ttcttctcgg
     4621 gaaaatcgac ctcgccggcc acttgcaccg cgtagggaga cgcgtcaaag cgcgtgattt
     4681 ttcgaattcc actgacgcca tgggaagtgg ccgcccagaa gttgtcccgc cccaccccgt
     4741 tgggactgac ggcgcccagg ccggtaatga ctactcttcg gctcatcgcg tttcgatggt
     4801 agcagtatct gccggccagg agacatcgat cgcgaggatt accccgccgg aacggcatga
     4861 gtgcgaatgt ccggttgagt gcggccgtgc ttccagtgca accatgccag gctgatgacg
     4921 cggtagactt cgttccgttc aaatggctcc atcagcaggt cgtatccgcc gaagttcagc
     4981 acttcacccc agagagattc gttcgccatc ctcgatgtga cgatgatgac aggtggcttc
     5041 tccacgccgt ccagcgcgta tagcaggtcc cgccaggtgc gcctgcccag gcggtcttcg
     5101 gcgatgacga caggcatggg tgtacggcgg atcacttcga ccgcttcttc gaatgtgtca
     5161 actgtgtgga gaatccaatt ggagttgccg aacagcctgt ggagggacag gcgatcttct
     5221 tcggacgata ggatagcaag agcggtaaca ctcttgcttg gatcgtgttc catggttacc
     5281 tccaccagtg cgatgctggc ggaacggccc gccggaacta ctgatcgagc cggcatcaag
     5341 acaccggtct ctttaattat cggtcatacc ggccgccggt tgcaattgca tttcgagtgc
     5401 gggttacctg cacatcggac aaacggagcg gagatacctt agacccggca acagttatac
     5461 aatgatggcg aacccggttc gtccgaggct acataaaatg cagactgatt caaccattcc
     5521 atcccgaaga tcctttcttc gcaaaggaag catcgccgga gcaggcgttt ccgcatttac
     5581 gatcatcaaa ccggaactcg tgagaggcgc cggcaacgaa aagttgaaag caggcgtggt
     5641 aggctgtggc ggccgaggca cccaggcagt ggtggacctg ctgaccggta gccagaacgt
     5701 cgacgtggtg gcgatggccg acgcgttcga ggacaaactg gaaggttcgc tgcggcggct
     5761 ccgcgcgcac aaagagtccg ggaaatgggg cgacaggctg aaagtggacc cggaacaccg
     5821 ttttgtcggg ttcgatgcct tcaagaaact tgtcaactcc ggcgtggata tcgtcatgct
     5881 ggcgacacct ccggggtatc gtccggagca ctttgaagcg gccgtcaacg ccaagaagca
     5941 tgtcttctgc gagaagcctt tcggaaccga tccggttggc gtacgccggt tcatgactgc
     6001 ggccaggaaa tccgaagaaa tgaaactgac ggtcgtgacc ggcgcgcagc ggcggtttgc
     6061 gcgcgactat caggaaacgg tcgaaaagat caacagcggt gccatcggag acatcgtggc
     6121 aacttatgcc tactgggtgg gtactcccgt cgtccagcag aaggcgggcc gaaatcccaa
     6181 atggggcgat atggagtggc agcaccggaa ctggtattcg tacgtgtgga tctgcggcga
     6241 ccagatcgtc gagcagcacc ttcacaacat cgacgtctgc aactggatca tgggcacgca
     6301 tccggtcaag gtcgttgcca cgggcggcgc gatctggaga ccgaagaccg aggtttacgg
     6361 taacatttac gaccacatca gcgccgattt cgaatatccg aacggcgtcc gcatgtccag
     6421 ctattgccgg cagtttccca gggaccggaa ctccgagatc cacaccaata tcagcgagct
     6481 cattgtagga acgaaagggc gcagcaacgc gcatgacctt ggcacgggca gcgggcagga
     6541 tgttcgcaca tccttcaggg atccattgga gaacccgtat gtgcgcgagc acaccgccat
     6601 ggtcgccagc attcgcggcg acgggccgta catcaaccat gcgatggcgg tggccgaaag
     6661 cacgatgacc tgcatcatgg ggcgcgaatc cgcctactcg gggctcgaga tcacctggga
     6721 catgatcatg aactcgaagc aggacttgca gccgaagcag tttggctacg acctgaagat
     6781 ggaagtaccg ccgctgccgg ttccaggcga gtataagttt gtctgacgag cgcggcggcg
     6841 cgtcagcaat cgcgcggggg aaggtcacac ccggggcgaa tttcgaatca tgcgggagcc
     6901 gagtaatacg tagtcggcgg cagagatcac aatagaaacc agcagcacca ggtagagcgg
     6961 gccgagagcc tgcgaaatcg cgggcaggct tgccgacgcg agaaaacata cgatggtgaa
     7021 gatctcgagg atagtgttaa tttttccgga aatggtaggt ctgaaagcgg tactgcgcgt
     7081 caacaggtaa accacaaagc acccgatcag gatcagcaca tcccgcagca cgacggcggc
     7141 cataagccat aacgggaggg aaacgaactg cccgcgaaat gccgaaagga cgacaaatgc
     7201 cgtcgaggtg agtattttgt cggctaccgg atcggccagg cggccgaact tggagcgctg
     7261 gccgaatctc cttgcaatgt acccgtcaaa ggcgtcggtg agcccggcca aagcaaagac
     7321 gcccagagct tccatatccc ggcccagaat ggcgaaccag gcgaatggaa ctatcaacgc
     7381 cagccgcaac agggtcagca ggttcggcac cgttcgccac gccgcttttt cagcaccgtt
     7441 ttcaacagcg gcggcagaga cttccggatg attccgggga agcatatccg gatgctaccg
     7501 ggttctcgcc ctatcgggca agccggacaa tccacagagc agtttcaatg aggcactgac
     7561 acgatcatga gaataggaat cacttacctg tatgcaattt ttcgttatgg ctatcctcat
     7621 tccgtcgcgg atgcgctccg ctcgataccg gacatccgca agcttgggtt ccgctttctt
     7681 gaaatggagg ggctcgggcg tccgtttctc cgggctcttt acaaggaccg gaacacactg
     7741 aggcaagtgg tggaggacaa cggcgtccac gtccacaatt tttgcgtcgt ggatcccgat
     7801 atggtcagtc tccatccagg caaacagaaa gcggcgctcg atggcttcaa gaagggcgcg
     7861 gagatcgcag cgatgctcgg aaccgagacg ctgcacctgg ccagctacgc tccgcccgtc
     7921 cggtacctcg atcagaagcc ctatgatctt ggagctgagg agggatacag ttttgccggc
     7981 ggctcgcgca ttcgcatccc gaaggatttc gactggaata gcgtctggat cgcgctggtg
     8041 gattcctgcc ggcagtgtgc ggatatcgcc gccaactacg ggaagacggt cattatggaa
     8101 ccgcgcgtgg gggaggtcat ctgttcggtc gattcgcagt tgcggctgat tgaagacgtc
     8161 gcccggccga acttcaaagc taatttcgat acggcgcact tcagcgcaca acgggagaac
     8221 gtcgtgttgg ctctggaaaa actccggaac cggtttgcca acattcatat cgccgacaac
     8281 aaccccaaag acaccggcca ccttgcaatc ggggacggcg tcatcgattg ggaggaattc
     8341 ttcgtcgtat tgaagcggat gaattacaca ggatacctcg ggctggacct gggcacgtcc
     8401 cgccacttgg ccgcggacta ccgcaaatcc ctggaccgaa tccaatccat cgcttcgaag
     8461 ctgaaaatca agatcgaggt gtaacgcgct gcacgtttat tctgcgttct tgatcatctt
     8521 ggcttgcgcc gttgcaaatg ccggtccaca cggaccattg cggacggcct gcgagactgc
     8581 cgatgctgtg gtgttgggca gcatcgatcc tcttgtgctc acttccgcgc cggcaagctt
     8641 catcttaacc atcgatggag taataaaggg cacgacaccc agaaattccg tattgacggt
     8701 aacctggcca ggatcgctca catcggctcg cataagccct cccaaatacc gggctttgtg
     8761 gttcctgcgc aacaccccgt ctggagttcc ggaaatctct cctataggag gaccaagagc
     8821 tccattgttc gcgtccggat tggctttgcc gcccaataac cggagcggcc gggaccaaat
     8881 tccaaccgga acctgtcacg aggtcgtatg ggcggttctc aaggatagcg ccgcctacat
     8941 cgatctatcg ccggcctgcc tgacggcaat ggaaacgctg ttaagggaag atcctccgga
     9001 gaccgccgcc gcgctccggg acttttccga atcgatacaa cgcttcgctc aatcggcatc
     9061 cgtaaacctc agagctctgg ccctcgcgtc gggcattcga cggcaggatg tgcaatcgct
     9121 ggcacaattc gcggcgcagg cgtcattgct tgcgaaatcg caagtcatcc accctgccgc
     9181 ggcggccgtt gccgcgtggc gcgatccgga tccggcgggc ctggcagcac tcggaacaat
     9241 ttctcagaca gcaggcgccg atactctcag ccgctccgcg gcacaggctc tcatgatgat
     9301 ccacacgaaa gatacggtgc cacatttagc caagctgcta tccagtaccg atccggaact
     9361 tcgaggcgcc gccatccggg gtctgagttt ttttgtgaag ggagtcccca ttctttcggc
     9421 tgagaacctc cggtccatgg catatttcgc ggaggttgag aatcccgaat atttcgacga
     9481 aacgatttcg ccgtatgtct ccataatccc ggtctcccct gcgaaagaag ccgaatacgt
     9541 aaatgcctgg caggcctggt ggcgccggat ggcaacacgg tttaattgac tcgacgacgc
     9601 tattgaatgc aggcatggct ctactacagt ctcgtggacc aggaaagcgt agaatgaaat
     9661 taggagactt gccatggagg tcacgctacc agccgatttg agaaggcagg tcgagcaaga
     9721 actcgccagc ggccggtatc acagccggga tgaactgatc gaacaggcgg tcaggcactt
     9781 ccttgatgag cgtcaacgtg ggcaacgtcg gctcgatgcc ctccggagaa tcggtcaggc
     9841 cgttgatcag gccggcatgt atgagcgagt gctggttcct ggcaaggaat gacgggctca
     9901 ccgctcgtcg ttatagatgc gtgtgtcctc gctaacttct ccctttgtga taccctcctg
     9961 cgcctcgctg agccgccgcg actctatgag ccgaagtggt cggaggagat cattcgtgag
    10021 acaactcgta cgttggaatc aaagctcggg tggccgagtc ctctgacggc tagtctccag
    10081 gctgaactgc gcgcccactt cggtgagaca tggatcagcg gccacgaagc actgatcccc
    10141 cagatgacaa acgatgagaa ggatcggcat gtgctggcgg ccgccgatca cggccaggcg
    10201 tccatcatcg tcactttcaa ccttcgccac ttcaggcgtg agcacctcga gccgtggggc
    10261 atctgcgcgt tacacccgca atcctttttg atcgagacct ttcgccagga agaatcgctg
    10321 ggaatgacga aactggagca acaagccgcc gatcgccggc gcagcttgcg ggaacttctc
    10381 catattctca gcgccaccgt gcccgacttt gtggcagtcg tttcggctgc gttctcaaga
    10441 gaatagaaat cggaatggcc gctactcgga gcccagaacg gccagcgttt tttccacgtt
    10501 tgaaagcaat tcctcgcggt gccggcgctc tgaaaattcg agatccgggc tccgatagta
    10561 accggcctgc tgctgccagc ggagaagaag ggcccggcgt gctccgttga cggatacgtg
    10621 acggccctcg cgcaacaggt acacggggtt ggcgtgggcc tggatttggg gttcgccttc
    10681 ctggctggcg ctggtggcgc gggctgcgac ccaggaactc tcctcgagat tgaaacgaaa
    10741 cggaaggcgt acctcatggc gcccttctcc ggctatcgag gctaccgtcc taccgttcac
    10801 gatgatttcc agcttttcga gcggccggtg atagcgcgcc accgcttcgc cttccaccgg
    10861 tttctgaacc ccgctccagt tgatgacggc gccaggggca ctgccgtcga ccgtcaaatc
    10921 cagcaagggg ccattggaaa caaccacgcg gccgattcgc acccctgccg cccaagcttc
    10981 ataagcggaa ggtttcggag cggccttcac cagagtgcgc tccggcccga gcagcggcag
    11041 ggagcgtggc cagggctttc ggttattcaa agggacggga aaatcgcttc cggcgattcc
    11101 cgtcacccga aaccccgcgt tcagcagctc ataccaggac tcggtcttga gcacaccaaa
    11161 ctggaatacc tcaatgaaat cgatgctccc gagcgcgagg tccatcagca gcgtggaatt
    11221 cttctggctg ccatcaaaat gcgcatagcc caccgtggcc ccaagactcc gcgccctggc
    11281 aaacagcacg gttggaaacg gatacgcttc gcgcgagttc gcgtaaacct ctccaatgct
    11341 caaagggcgc aaaatctcac gaggtccgag gaggttcacg tgaccataga aatggctgcg
    11401 tgcttccatg ccggaacgaa tcgaccgccc ttccatccgg gcttccgcgg cctctccaaa
    11461 gccatactgc atggccgcgt cctgctgccg ctgtagttgg agaaaattgc ccacggagag
    11521 atcttcgccg tcgatccatc ccagaaaaac cggatcgtcc tcccggtgac gcaccaggtg
    11581 gatgtggtcg tccgcggaga gccattgctc atgctccgtt aggggagcaa ggtcgagttc
    11641 aatgcgctgc ttgccgccgg cgcgcaactc gaactcctga acggcaggct tgtagaacaa
    11701 gcccctgtac gcttccgccc ggtagcgccc cgccggtaaa tcaaaggcgg cggtgcctcg
    11761 aagcagaagg aaatgggatt cgccgttcgc cgtgatctcc agcgtggctg gatccgccgc
    11821 ccggcgccaa agcacttcgc ggtagaacag atcgacgcgc agggcaagca ggctgtcaac
    11881 cggactgaag cgaaacggcc gcccgtcttt gaacagatac acgcgggctg gaacagtccg
    11941 gccgctattc gcatcgcgag taacaagttc aagcgtagcg gcgaaagacg gccgcagcgt
    12001 ctcgcggaat tgggcaaaag ccagagccag gcaaaacgcg agcaccaggc caaaacggag
    12061 aacaatgact cttgcgcttg gcacaagaga gtaatatcac agccgcaacg gtattatgtc
    12121 tcgggaacgg tcagcttgcc attcctgacc aggtaccgga aatccacgtg aagcgtcgta
    12181 tcgggcagga agaaccagcg cacgaaaccg ccgcggacct tccccccgat ctcttcattg
    12241 tctccgaggc tcaagcggac gacgccggcg ccgtaaccgt aataaggaat cagcccgctt
    12301 ccctccggca cagtcaacgc cgggttgcat ccgaggccga attcacggaa gcgcatcgcc
    12361 gcatcgccgc ccgccgcgag cgcggctttc acggcgtcca aaccctcctt ggctcgcaca
    12421 ccggttacac ggccgttatc gagtagaatg actacacctt tcgcgactgt atcgtcgaac
    12481 ctagcttcgg caagtccgat gctgccgaag gcgctgtctt cgaccggcgc gacgcgcacc
    12541 acacccgccg gcaattcgat atggcgatcc acgcgcacct gcgccgcctg cgcgcgctcg
    12601 ggggaggcgt caccgtcctg aatattaaag ggccgttttg cgattcgaaa catcacatcg
    12661 gtgccgttcg gagactgtat gcgaaccgtg cccatgcgaa gaatacgaat ggcgcggtcc
    12721 tgtacggcgc gcagggcttg atgatcgatg ttcaaagccc gcatgtacag ggcgtcgaac
    12781 tccggcggat gcgcagtggg caaaccatcg ggatgccggg taccgtcaga ccagtgaaag
    12841 tgcagctcac gccgtgtgcc gcctttatcc acccaatcga cgagatgccg ttcttccgcg
    12901 ggtgccagcg cccgcgcgga agggccgagc ggtaacctga taaacacgtc gacgcgcccg
    12961 gcagttgtgg cggaaacggg cgcggccgcc gggagcggtt ccagccacac ggcgcctgcc
    13021 gagcggacgt tttgccgcaa cagcggcagc agttccgcga agtattcagg atcgaaccgg
    13081 gccagcacac gctcaccacg gcggagcttc aacggttcca gaatgcgcgc cgcaatgcga
    13141 gggtaatcca cagaagaagt ggaatcggcc gcggcgagcg ccgagggcac ggcgggcagc
    13201 atggaacaaa acaagcggcg gttcataacg gatcagatcg ccatcaccgc gcctgtatcg
    13261 gcgctggtca cgtgatgcga ataccttgcg agccaaccgc gcttcacctt tggttcgaag
    13321 ggaggcaaag cagccaggcg gcgctcgatt tcaccggcat cgactttcaa ttcgatccgg
    13381 cgcgttgtcg tgttcacata gatgacatcc ccgtcacgga cggccgcgat gggacctccg
    13441 gctgccgctt ccggcgccac gtgaccgatg caaaggccgc gcgtgccgcc cgaaaatctt
    13501 ccgtccgtga tgagcgcagc gcgggtgccg gccccgacca gcgccgccgt gggggaaagc
    13561 atctcctgca tcccaggtcc gccacgcggg ccctcatagc ggatgacgac cacttctcct
    13621 tcgcgcactt ctctttcgag gatgcccttt aacgccgctt cctgagattc gtagactcgc
    13681 gcgggccctt cgtactccat catcgccgcg tccacgcccg cggtcttgac gacagcgcca
    13741 tgcggagcga gattcccgaa aagtacggtc aggccgccgg ttgcggaaaa cgcatttgaa
    13801 acacgacgga tcacgtcgcc atcggcatcg ggcgaggcgg tgacatattc gcccagccgc
    13861 ccggccactc ccgcggtatc caggttcaga acggtcttcg catgacacgc caattccttc
    13921 aggatggtgc cgataccgcc ggcacggtga acgtcttcga catgcatctc cggccggctg
    13981 ggcgacacct tgcagatatt gggtgtggta tccgagatct cgttcaaccg gtccagccgg
    14041 taatcgatcc ccgccgaatg cgcgagcgcc agcgcatgca ataccgtatt cgtcgacccg
    14101 cccatcgcca tgtccagaac aaaggcatta tcaagcgcct ggcgcgtcac gatatccagc
    14161 ggccggacgt tgttatcgag acacgatttg agcgtgcgcg ccgcccggcg ggtgagttcg
    14221 atccgctccg gattcagctc cgtgatcccc tcggcgcggt cgacccattg ctccgccgta
    14281 atggtaccat tgccgggaag cgcgagaccg atcacttctc caaggcagtt catcgaattc
    14341 gcggtgaaca tcccggagca gcttccgcat cccgggcacg cgcgctcagc caggccgcgg
    14401 agaccctgct ccgtcatctt gcccgccgta tatttgccta cgccctcgaa gacggagatc
    14461 agatccgagt tgcccgttcc cgccagcatc ggaccaccgc tgacgtacac cgcggggata
    14521 ttgatgcgga ccatcgcgtt gtacatcgcc gggacgatct tgtcgcagtt cccgattccg
    14581 atccaggcgt cgcaggggtg ggctccgata atggattcga tctggtccgc aatcagctcg
    14641 cgcgaaggca gagagtactt cattccgaag tggcccatcg cgattccatc gcagatagcc
    14701 gcccctacgt tcgtgtacca cacgttgtag cccagcgact tcagttcctc gaacacgatg
    14761 gccccgatac ggtcgagatg agcgtgtccg gggatctggg tggtatagga attgataatc
    14821 gtgacgaacg gacgcttcaa atcgtccatc gaccggatca cgccttcggc ccgcatcagg
    14881 gaagccgccg gtgtcatgtg aacgccctcg gtcaggatac ggctgccgcg cctgcgaatg
    14941 ttttcgatgc cttcgttcat tactgacatt gtgccagtaa cggagtctcc gtgcggctat
    15001 actgactatt cgggacctta actctggaag gaggaatcca cgatgccggt cgtctcaaga
    15061 cgggagtggc ttgcgtccct gtttgcctgc ccggccctgt ccatcgccgc gccggatccg
    15121 gaggcgtgga ttccgctgtt cgatggcgcc tcgctcgacg gctggacggc tggcgaaaac
    15181 cgcgattcgt tccgcgtgga gggaggcacg atcgtcgccg ccgggccgcg ctcgcatctg
    15241 ttttacacgg gcggcattcg cgaagccagc ttcaggaatt tcgagtgcag agcggatgtg
    15301 ctgccgcacc cgggcgccaa ttccggcatc tacttccaca ctcgcttcca gaaacaaggc
    15361 tggcccacag ccgggttcga agtgcagatc aataactcct atggcggcga gggccgttat
    15421 cgcgagcgca gaaagacagg ctcgctctac ggcgtccgga acgtctacaa ggcgtttgcc
    15481 cgtgatggcg aatgggcaca gttgcacata acggtacaca gcaaacaggt gcaggtccgc
    15541 ttgaacgatc tgcttctggt ggattatgtc gagcccgatc ctcccgtccg cgccgataaa
    15601 accatggacc gcgtgctcaa tcgcggaacg ttcgcactgc aatgccatga cccgggctcc
    15661 cgtacgcatt tccggaatat cgcagtgaag cccttgccag acgacgttcg tgcatccgcg
    15721 gcggcgcccg aagtagacga cgtctatcgg cagattctgg atctgagcgc gcgcaatttt
    15781 ccggtggtgg actaccacac gcacctgaag ggcggcctca ccattgacga gatgctgcga
    15841 gagtcgcgcc gtctgggcat ccaatacgga gtcgccgtga attgcggtgt gtcgtttccc
    15901 atccacaatg acgctggatt gaacgatttc ctggagacca tgaaagggca accggtcttc
    15961 attgcccttc aggccgaagg ccgggaatgg gtcaacctcg tcagcagtga aatggtggcg
    16021 agatttgact atgtttttac cgactccatg acctacaccg atgacgccgg aaagcgaatg
    16081 aggttatgga tcaaggaaga agtgggcgac atccgcgacc cgcaggcatt tatggagacc
    16141 tacgtcgatc gcaccgtcgg cattctaagc cgggaaccga ttgatattta cgcgaacccg
    16201 acgtttcttc cggacgtaat tacgtctcaa tatgatgtgc tatggacaga ggagcggatg
    16261 cgcagggtgg tggaggcggc agccgcgaac ggcgtggcaa tggagatcaa cagccggtac
    16321 cggctgccca gcccggcctt cattaaaatg gcgaaggccg ctggagtaaa attctccttc
    16381 ggcacaaata atgacgccaa atatctcggg cgctgcgaat acgggctccg gatggtaaaa
    16441 gagtgcgggc tcgattggca cgatttcttc atcccgaagg aagacggcaa caagcccgtt
    16501 caggttcggg gtctcaaagg ttgagcgatt atttttgaga gggaatgtat gtcagattta
    16561 tccagacgcg attttgggcg gttggccttc cttggggcgg cgctcacgac gaatcatgta
    16621 gcgctctccg gcgcgaacgg caacatcggg atcggtgtcc agctttactc ggtgcgcaag
    16681 gatgcagctg ccgatctgcg cggcgtactg gctgccatcg gccggatggg ttatcaggcg
    16741 gtggagttcg ccggctatta cgaccatgac gcgaaggccg tgcgaaagat gctggacgat
    16801 gccggcctga agtgctgcgg gtcgcacatc ggcttgcctg cccttcgcgg cgacgagtta
    16861 tccaaaacgg tcgagttcaa ccgcatcatt ggaaacaaat acctggttgt gcccggcctg
    16921 ccgaaggaaa accgcgcttc gcgccaggcg tggatcgaga cagcgtcggt cttcaatgag
    16981 atctcggcga aagtgaagcc ccacggaatg cgcgtgggtt atcacaatca cacgatagag
    17041 tttcaacccc ttgacggcga gaagccctgg gacacgttct tcggcaggac gaacaaggac
    17101 gtcatcatgc aactggacat cggacacgcg cagcgcgccg gcaacgatcc catcgacgtg
    17161 atgaagaaat acccgggcag ggcggtcacg gtgcacgtga aggagttctc tccctcagac
    17221 aaaaacgcgc tgatcggtga gggacagatc aactggaaag gtttcttcca ggccgcgcaa
    17281 accgtgggcg gaacggagtg gtacatcatc gaggaagaaa gcggcgcata ccccggatta
    17341 gagggcatcg agaagagtat caagaatctg aaaaaactcc tctcctgagc ggcgccgtta
    17401 attcgacggc gctatccgcg ccagggcgcg cgtggccgct tcgatcagcg cttcggcacc
    17461 gacgggcgcg ccggttgcgc ccttcatcca cagatccggg gcgatgcgcc cctgcttgct
    17521 tacccgctcg acttcagggc caatcttgcc ggccttggcc atgtgttcct ccaactgatg
    17581 cgcgatgagg tggccgatcg ggtagtccgg caggtagagg tagcgctcga tcatgtgcga
    17641 gtagatgccc agcagaacca catccttgtg gcggaacacc ggcgcatagt gcctgttcca
    17701 cacatcggtg gcgatttcga tcaccgcttc ccttagctgc tcgggcgtcg catccggctg
    17761 atcgtacatc cagtgccata cggccatgtc caccagcgcc acgccggcga tctcgtacgt
    17821 cgcccagaag tcgttcagaa tcttgagcgc ttcggcgctt tcatcggtat ccttcaatcc
    17881 gataagctcc aggtccctgg cctggaacac aaacgcgagc gcttcggtaa aagcggtatt
    17941 cggcacgccg ttgagcagcc agtggtcgat gttcttcagc gagaacgtct gctctacgtt
    18001 gtgccccatc tcgtgaacgg caatgttgta gcctttgtaa tccatgccgt ccgcaccaac
    18061 gcgagtacgc agccttgccg gagccgtacg caactgcgcg ccccacgcat gtccggagcc
    18121 gcgggccggt tgcacgacga tgttgctcgc aagataatcc gcacgttccg cgctgaagcc
    18181 caggtcccgg aacatgcgcg gcatgtcttt ctcgtatgct tccggcgtgg gataccgctt
    18241 tcgcgtgatc tcatccagtt cggcttccga gtacttgccg cgcggcttga agccggcata
    18301 ccagatatcg aacggctcga gcggccggcc aagtctctgc tcaatcaact tcgctacgcg
    18361 ggggatcagc ggagatgtca ggatctgctc aaacatcgct tgaacacgtt cttccggcaa
    18421 ctcgcgctct tcatcgaaaa tccgggccat gtgcgtaggc gccgccggcg agtagggatc
    18481 gatctgtctc aacgcgcgga atgtgccgag caacatcgcg taccgggtat caggctcgcg
    18541 ctcattcgag gcttggcgcg ccggccgcgg cacctcgctg taatccttca cttcagaccg
    18601 gctgaccgag ttccaggata gattccaatc gacggttgga ttgttcacca ccgcttgcgg
    18661 gatggtttgc cggacgatgt gctccatgac gcgcgcaatc attcgctggc gcggcagccc
    18721 gtctttttcc gtgtagtcgg ctttgatctg atcgcgcaga ttccaatgcg agagcagccg
    18781 catttttgca gggaacaacc gttgtccttt ttcatcgagc agatggttca tccagatgtt
    18841 gtattgcgcg atgtactgtt cggcttgtgc cccggcctcg gcgatcgcca aattcacctc
    18901 gggaggcact cttcgcgaga agcgctgagc caggcgcgct tcggcccact cgcggcggct
    18961 ccactgctca cctcgcgcca gccgctcttc gagcgtggtc agcgggaaat tcaacaggac
    19021 aacgaacgcc agcttgtttt tgaaaaagtc ttctttcgta tgcgccgatg ggtcgtaggc
    19081 ggcgaagatt tcgtcatagg gcatgatcgg gccgcctcgg tccagatcaa cctggagccg
    19141 gaaggcgatc tggatttcca gcatatgccc gtcaagctgc tcgaacaggg tctcatagcg
    19201 ggagaaaacg gtgtcgaggg ctttctggtc gccggcgaag ttcgtccgga cgaattcctc
    19261 gaacgcgttg cggtcgccgt cgtccgcgcg ccagaacccg gctacctgtt tcaatccgcg
    19321 ttcgacacgc acgcgctgcg tggggccgta tttgtccacc aattcttttt cgagcgcgga
    19381 aacggcgccc gcgatccaat ccggacgggc gttctgcccc tgcgctttga gcggcggtga
    19441 aatcatggca aaccccagca gaaacggcag caacacgcgc aagtgcggca cgaaaaatct
    19501 cctgtgcacg gctgtagttc cgcgcagatt tagtataacg tgcggtctcg cggcagactg
    19561 tggcgataat ggagtcaaat agcgcccaca tgcgaatact ggttctgaac ggcggatcga
    19621 gtacgatcaa agcttccctg tacgacgtcg aaggcggcac catgtccggt gccgcccagc
    19681 gtcccatctg gcgtgtccat gggggctggg ggaacagcaa tgggaccgca cgaattcatg
    19741 ccgagggtcc gggcggcgtg gaagatatgg aactgcgaat cggcgctccc ggggaagtta
    19801 tcgaacccgc gctgggcatg ctcctgaaag ggaaggctca agtgctgcgc gcatggacgg
    19861 agatatccac tgtggggcat aggatcgtcc atgggggcag ccgtcttcgc gaaagcgcgc
    19921 tcatctcgga ggaagtgaaa tccgggatct ccgaagcggc cgaattcgcg ccggagcaca
    19981 actggtttca actggaaaca atccataccg tcgaacggct gctcggcccc ggcgtgcctc
    20041 agttcgctgt cttcgatacc gctttccacg ccactctgcc ggagcgcacg tttgtctacg
    20101 gaggtcctta cgaatggctt gaccgcggca tccggcgata tgggttccac ggtatcagcc
    20161 atcaatacgt gttcggacgc gcggcggaaa ttctgggcca tgatgcgtcc gggctgaaac
    20221 tggttagctg tcatctcgga aacgggtgtt ctctcgccgc gatcgatggc ggaaagagcg
    20281 tcgacaccac gatggggttt acgccgctcg acggcctgat tatgggaacc cggtcggggt
    20341 cggtcgatcc cggcattctc atctacctga tgcagcgcgg cggatacaca gcggaccaac
    20401 tgaaccgaac cttgaacaag gagtcggggc tgaagggact ctccggcatc tcgggcgaca
    20461 tgcgcgaaat catggccgcc gtcgaagtgg gaaaccgccg cgccgggctg gcattcgacg
    20521 cttacataca acgcttatgc cgcgagatcg gcgggatggt t
//
LOCUS       DASTMK010000434        10196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_213294, whole genome shotgun sequence.
ACCESSION   DASTMK010000434 DASTMK010000000
VERSION     DASTMK010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213294"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3885
                     /locus_tag="VFQ79_18610"
     CDS             <1..3885
                     /locus_tag="VFQ79_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0141741.1"
                     /translation="LTDSVNSMAGNLTNQVRNIAEVTTAVARGDLSRKITVDVRGEIL
                     ELKNTINTMVDQLSSFASEVTRVAREVGTEGKLGGQAVVSGVAGTWKDLTESVNSMAS
                     NLTNQVRNIAGVTTAVARGDLSTKITVDVRGEILELKNTINTMVDQLNSFASEVTRVA
                     REVGTEGKLGGQAVVTGVAGTWKDLTESVNSMAANLTTQVRGIAKVVTAVANGDLKRK
                     LILETKGEIAELAETINGMIDTLAVFTEQVTSVAREVGIEGKLGGQANVPGAAGLWRD
                     LTNNVNQLAANLTTQVRAIAEVATAVTKGDLTRSITVQAQGEVAALKDTINEMIGNLK
                     ETTRKNTEQDWLKTNVARFTRMLQGQRDLMAVSGTVLKEMAPLVEAQHGVFYISSDAN
                     GDDSEFKLLASYAYRERKSLSNRFGLGEGLVGQCALEKQRILLTDVPGDYIKINSGLG
                     EAAPLNIVVFPVLFEGDVKAVVELASFHSFSAVHLAFLEQLAESMGIVLNTIEATMRT
                     EELLTQSQALAEELQSQQKELTEGNRRLEEQARTLQASEELLRHQQEKLQHTNSELQE
                     KARLLADQKAEVERKNHEVEQAKEALEEKAEQLALTSKYKSEFLANMSHELRTPLNSL
                     LILAQMLAEDNENRLTGKQVKAAQTIHQSGVELLSLINDILDLSKIESGTILLDLEQV
                     FVTDLKDYVTRTFSHVAEEKGLAFETELGPDLPKTLYTDPRRLQQVLKNLLSNAIKFT
                     DDGGVRLKIDRASGGWRADVKSLNEAETVVVFSIVDTGIGIAEDQQQVIFEAFRQVDG
                     TSSRRYSGTGLGLSICRELAALLGGDIQLESKPGEGSTFSLYLPQNYAGSPASLKRLS
                     SPRRKPETPRETSQQERAGAAHARVVSMVPVEVEDDRHNITPGDRVLLVIEDDPTFAR
                     ILVDLARQAGFKTVVALWGDRGLALARELKPDAITLDIRLPDMAGWTILDRVRHDPET
                     RQIPIHIISVDEDRRRGLSLGASSYLEKSNSKERFVEVFERILESVERRQRELLIVEP
                     DGERRGRIQEVLVGGDVRSVFVSSGAEAVEAVKTGSFDCVVLGLEMGDIDPLELIESW
                     QNSPETRELPVVVYIDNNLTPEQEHTINRLARHALVRAVCSLDRLLEETAVFLHRVES
                     AMPEESRRKLANMPKDSVIAGGKILVVDDDVRNLFALTSVLERHKLEVLHAEGGPEGL
                     EILKRNPDVDAVLMDIMMPEMDGYETIRQIRQIPEFRGLPVVALTAKAMGGDREKCIE
                     AGASDYITKPVNLDELIWMLRAWMPRRAERRA"
     gene            3837..5207
                     /locus_tag="VFQ79_18615"
     CDS             3837..5207
                     /locus_tag="VFQ79_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0141742.1"
                     /translation="MDAQGMDAEACRASRVDFLVLTPMGCMLGGESRLNNKMAGYGVG
                     LGQSRELKAVANQKARILVVDDHPGNLEAVESILEELGQEIVKAGSGKEALRILLKVD
                     FAVILLDVKMPGMDGFETATLIRERERSRYTPILFLTAHREEEEHLSRGYYAGAVDFL
                     YKPINPEILRSKVAVFVDLNKKTELLKKHAEMLEIRNRELEKLVAEKERAEESIRRLN
                     LQLEQRVAERTRELSRSNEDLRQFAYAASHDLQEPLRTVGNFTELLSQRMKNVEDRDA
                     QEFMAYIIDGVRRMNTLVKDLLAYSQVSHGPIESVNPACAEAVFQAAAMNLSVAIRES
                     GATVTHDPLPDVLIDFGQLTQIFQNLIGNAIKYRREQPPAVHVSAEETETHWVLSFRD
                     NGIGIDPAYKDQVFGIFKRLHGREYPGTGMGLAICKKIIDRYGGQMWVESQRGQGADF
                     KFSIPK"
     gene            complement(5679..6602)
                     /locus_tag="VFQ79_18620"
     CDS             complement(5679..6602)
                     /locus_tag="VFQ79_18620"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0141743.1"
                     /translation="MRPASELEQRLTCRRKAEELHRKGRSAHGRGDYILRHDRLVGPV
                     LRTALQAAGLYRRGCRNAVRPVVRHTTVRIPQLPSSFSHFRILHMSDFHIDGVQGLAE
                     VIAETVSRHPVDACVLTGDYRFETEGEYDLVLPRMRMALSRVQSHYGIIGILGNHDCA
                     DLAVDLSKIGVRMLINDAIELRKGVDSIWIAGVDDPHYYGCDDLSQALKEVPDGSTRI
                     LLAHSPEIYEEAASAGIQLYLCGHTHGGQICLPWLGPVVLNAKCPREYTCGAWHHRSL
                     QGYTSAGVGCSMLPVRYHCPPEITVLELVAD"
     gene            complement(6862..>10196)
                     /gene="metH"
                     /locus_tag="VFQ79_18625"
     CDS             complement(6862..>10196)
                     /gene="metH"
                     /locus_tag="VFQ79_18625"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU0141744.1"
                     /translation="ATSLVLAEYGLEQYAYEINREAARLAREAAAEFSTPNKPRFVAG
                     SMGPTTKAITVTGGVTFAELERTFHEQAKGLIDGGADVLLIETCQDTRNVKAALLAVG
                     RLGRELGRKIPAMVSGTIEPMGTMLAGQTADAFWVSVSHADLLSIGLNCATGPEFMTD
                     HIRTLNDMATTRVSCYPNAGLPNEDGKYLETPTSLAAQLERFVDHGWLNIIGGCCGTT
                     PEHIRAIVQMVEGKAPRIVKPSPNRAYYSGIELVEAEETNRPLLVGERTNVIGSRLFK
                     NLIAEEKWEEASEIARRQVRNGAQVIDVCLQSSERDEINDIPHFYEKLIRKIKAPVMI
                     DTTDAKAVEHALTYCQGKSIINSINLEDGEEKFERLCPIAKAYGAAVVVGCIDEDKLQ
                     AQAFTRERKLAIAERSVRLLTEKYGLAVGDIIIDPLVFPCATGDENYIGGAVETIEAI
                     RLIKEALPFVKTVLGVSNISFGLPPAAREVVNSVFLYYSTKAGLDLAIVNSEKLERFA
                     SIPAEERRLAEDLLFNTPPREVPADHRDANLLRAAPEDWRQQSREQKIAINQFHIAAI
                     AEHFRTATGKQKKTAVALSLDERLANYIVEGSKDGLIADLDTKRAEGAAPLEIINGPL
                     MAGMNEVGRLFNNNELIVAEVLQSAESMKAAVAYLERFMEKSETSTRGSIVLATVKGD
                     VHDIGKNLVEIILANNGFRVINLGIKVPPDELIRAYNEHKPDAIGLSGLLVKSAQQMV
                     ITAGDFHEAGINVPLLVGGAALSERFTKSRIAPSYSAPTFYAKDAMTGLRLLNEIMNP
                     DTRTGMLAAHTWEEPAPPVETASPALVPSGNGRSPKVATEIPIPAVPYLDRRMRAIPN
                     LPEVWSYINPFMLYGRHLGFRGNFEKALAERDEKALKLFHDVDEVKEYAKSFMKVRAV
                     WQFFEAERDGNSILLFSPGGSEPVHVFRFGRQAKEDGLCLSDYILDPKDGQRDHLALF
                     VVTAGEGIREHSEAAKEQGQFFKAHALQALGIETAEATAEWLHRRIREDWGFPDPVDL
                     TMQQRFTSRYRGKRYSFGYPACPNLDDQVGVWKLLQPDEIGVHLTEGMMMEPEASVSA
                     LVFHHPDCAYFTALETAEPVGV"
BASE COUNT         2170 a         2886 c         3038 g         2102 t
ORIGIN      
        1 ctgacggaca gcgtcaattc gatggcgggc aacctcacga accaggttcg caacatcgcc
       61 gaagtgacaa ctgccgtagc gcgcggcgac ctgtcgcgca agatcaccgt ggatgtgcgc
      121 ggcgagatcc tcgaattgaa gaacaccatc aacacgatgg tcgatcagct cagctccttc
      181 gcctccgaag tgacgcgcgt ggcgcgcgaa gtcggcacgg agggaaaact cggaggccag
      241 gccgttgtca gcggcgtggc gggcacgtgg aaggacctta ccgaaagcgt caactcgatg
      301 gcaagtaacc tgaccaacca ggttcgcaac atcgccggag tcaccactgc cgtagcccgt
      361 ggggatcttt ccaccaagat caccgtggac gtgcgcggtg agatcctcga attgaaaaac
      421 accatcaaca cgatggtgga tcagcttaat tcgtttgcct ccgaagtgac gcgcgtggcg
      481 cgcgaggtcg gcacggaagg gaaactcgga ggccaggccg ttgtcaccgg agtggcaggc
      541 acgtggaagg accttacgga aagcgtcaac tcgatggcgg ccaacctgac cacacaggtt
      601 cgcgggatag ccaaagttgt aaccgcggtg gccaacggag acctgaaacg caaactgatt
      661 ctcgagacca aaggtgagat cgccgagctg gcggagacca tcaacggcat gatcgatacg
      721 ctggccgttt tcacggagca ggtcacgtcg gttgcgcgtg aagtcggaat cgagggcaag
      781 ctgggcggac aggcaaacgt gcccggcgcc gcaggccttt ggcgcgattt gacgaacaac
      841 gtcaaccagc ttgccgcgaa tttaaccacc caggtgcggg cgatcgcgga agtggcgact
      901 gctgtgacga aaggcgacct gacacgatcc attaccgtgc aggcacaagg cgaggtggcc
      961 gcgctcaagg acacaatcaa cgaaatgatt ggaaacctga aggagaccac ccgaaagaac
     1021 acggagcagg actggctgaa aaccaacgtt gcgcgcttca cccggatgtt gcagggtcag
     1081 cgggacctca tggccgtgtc tggcacggtg ttgaaagaga tggctcctct cgtcgaagcg
     1141 cagcacggtg ttttctatat ctcctcggac gcgaacggcg acgattccga attcaagctg
     1201 ctggcaagct atgcataccg ggagcggaag agcctttcga atcgtttcgg actgggcgaa
     1261 ggcctggtgg ggcaatgcgc cctggagaaa cagcgaatcc tgctcacgga cgtgccgggc
     1321 gattacatca agattaactc ggggctcggc gaagcggcgc cgctgaacat tgttgtgttc
     1381 cctgtccttt ttgaaggcga tgtaaaagcc gtcgtagaac ttgcgtcgtt ccactctttc
     1441 agcgcggtgc atctcgcttt cctggaacaa ctggcggaaa gcatgggaat tgtgctcaac
     1501 acgattgagg cgactatgcg caccgaagag ttgctgacgc agtcgcaggc gctggccgag
     1561 gagctgcaaa gccagcagaa agagcttacg gaggggaacc gcaggctgga ggagcaggcc
     1621 cgcacgcttc aggcatcgga ggaattgctg cgccaccagc aggagaagtt acaacatacg
     1681 aactccgaac tgcaggaaaa agcccgcttg ctggcggacc agaaggcgga agtggagcgc
     1741 aagaaccacg aagtggagca agcgaaggag gcactggaag agaaagcgga gcaacttgcg
     1801 cttacatcca agtacaaatc cgagtttctc gccaacatgt cccacgagct gcggactccg
     1861 ctgaacagcc tgctgatact cgcccagatg ctcgccgaag ataacgagaa cagattgacc
     1921 gggaagcagg tgaaagccgc gcaaacaatt catcagtccg gcgtcgaact gctctcgctg
     1981 ataaacgaca tcctcgacct gtccaagatc gaatccggaa cgatcctcct cgacctggag
     2041 caggtctttg tgaccgacct caaggactac gtgacccgca cgttctcgca cgtggcggag
     2101 gagaaaggtc tggcattcga gacagaatta ggtccggatt tgccgaagac cctatatacc
     2161 gatccgcggc gattgcaaca ggtactgaaa aatctgttgt cgaacgctat caagttcacg
     2221 gacgacggcg gcgtgcggct gaagatcgat cgcgcatccg gaggctggcg cgcggacgta
     2281 aaaagcctga acgaagccga aaccgtggtg gtcttctcca tcgtcgatac aggtatcggc
     2341 atcgcggaag atcagcagca agtgatcttt gaagcgttcc gccaggtcga cggcacttcg
     2401 agccgccgtt atagcgggac tgggctcggg ctgtcgatct gccgcgagtt ggcggctcta
     2461 ttgggcggcg acattcaact tgagagcaaa ccgggcgaag gcagcacatt ctcgctgtat
     2521 ctgccacaga actatgcggg ctctccggcg agtttgaaac ggctctcctc tccccgccgc
     2581 aaacctgaaa caccccggga gaccagccaa caggagcgag ccggggcggc tcacgcgcgg
     2641 gtcgtctcca tggttcctgt cgaggtggaa gacgaccggc acaatatcac accgggagac
     2701 agggtgctgt tggttattga agacgacccc acgttcgcgc gaatactcgt ggatcttgcg
     2761 cggcaagcgg ggttcaagac cgtggtcgcc ctttggggcg atcgcggact ggcgctggcg
     2821 cgcgaactga agccggatgc tatcacgttg gatatccgcc tgccggatat ggcgggctgg
     2881 acgatactcg acagggtgcg gcacgacccc gagacccggc agatcccgat ccatatcatc
     2941 tcggtagacg aagacagacg ccgcggattg tcgcttggtg catccagtta tctggaaaaa
     3001 tcgaacagta aggaacgctt cgtggaagtg ttcgagcgca ttcttgaatc cgtcgaacga
     3061 aggcagcggg aattgctcat cgtggagccg gacggcgagc ggcgcgggcg cattcaggag
     3121 gttctggttg gcggagatgt ccgcagcgtg ttcgtctctt ccggcgcgga ggcagtcgag
     3181 gccgtcaaga ccggctcgtt cgattgcgtg gtcctcggat tggagatggg tgatattgat
     3241 ccgcttgagc tgatcgaaag ctggcagaac agtcccgaga cacgcgagct tcccgtggtg
     3301 gtttacatcg acaacaatct tacaccggag caggaacata caataaaccg gctggcccgg
     3361 cacgccctgg ttcgcgctgt ctgctcgctt gaccgtttgc tggaggagac agcggtgttc
     3421 cttcaccgtg ttgaatccgc catgccggag gagtcgcgac ggaaattggc aaatatgcca
     3481 aaggactccg ttatcgccgg cggcaagatt ctggtcgtcg atgacgatgt ccggaatttg
     3541 tttgccctca ccagtgtgtt ggagcggcat aagctggagg tactgcatgc ggaaggcggg
     3601 ccggaaggtt tagaaattct gaaaagaaat ccggatgtcg atgccgtcct gatggacatc
     3661 atgatgcctg aaatggacgg ttatgaaacc atccggcaga tccgtcagat acccgaattt
     3721 cgtggcttgc cggttgttgc gcttactgcg aaggcgatgg gcggcgatcg tgaaaaatgt
     3781 atagaggccg gtgcgtccga ttacatcacc aagccggtca acctggatga gttgatttgg
     3841 atgctcaggg catggatgcc gaggcgtgcc gagcgtcgcg cgtagatttc ctggtcctga
     3901 cgccgatggg gtgcatgctg ggcggagaat cgaggttgaa taacaagatg gcgggttatg
     3961 gggttggtct gggtcagtct cgagaactca aagcggttgc taaccagaaa gcaagaatcc
     4021 tggttgtcga cgaccacccg gggaatctgg aggccgtgga gtccattctg gaggaactgg
     4081 ggcaggagat cgtcaaagcc ggctccggca aagaggcgct tcgaattctg ctgaaggttg
     4141 attttgcggt tatcctgctc gatgtcaaaa tgcccgggat ggacgggttc gaaacggcga
     4201 ccctgatccg cgaaagagag cggtcgcgct atacgccgat cctgtttctg accgcccaca
     4261 gagaagagga ggaacatctt tcgcgcggtt actacgccgg tgctgtcgat tttctgtata
     4321 agccgatcaa cccggaaatt ctccgctcca aagtcgcggt cttcgtcgac ctgaataaga
     4381 agacggaact gctgaagaag catgccgaga tgctggagat ccgtaaccgt gaactcgaga
     4441 aactggtggc ggaaaaggag cgggccgagg aaagcatccg ccgcctgaat cttcagctcg
     4501 agcagcgtgt agcggagcgc acgagggaac tcagccgctc caacgaagac ttgcggcaat
     4561 tcgcctatgc cgcttcgcat gacttgcagg agcctctgcg taccgtcgga aatttcacgg
     4621 aactgctgtc gcagagaatg aagaacgtcg aggatcggga tgcgcaggag ttcatggcct
     4681 acatcatcga tggcgtgcgc cggatgaaca cgctggtgaa agacctgctc gcgtattcgc
     4741 aagtctcgca tggccccatc gaatcggtga atcctgcctg cgcggaagcg gttttccagg
     4801 ccgccgccat gaacctcagc gtcgctatca gggaaagcgg cgctacggtt acccacgacc
     4861 cgcttccgga tgttctgatc gatttcggcc aactcacgca gatctttcaa aacctgatcg
     4921 gcaacgcaat caagtacaga agggaacaac cgcctgccgt gcacgtttcc gccgaggaga
     4981 ccgaaaccca ctgggtgctt tcgtttcgcg ataacggtat cggaatcgac ccggcttaca
     5041 aagaccaggt ttttgggatt ttcaaacggc tgcacggaag agagtatccg ggtaccggga
     5101 tgggactggc aatctgtaag aagatcatcg accggtacgg aggacagatg tgggtggaat
     5161 cccagcgggg ccagggagcc gatttcaaat tctccattcc caaatagagc tgcgacacat
     5221 caagtgtgcc gaaatgaaac accggctcca aaatgcagcg tgacgcctac aagcgggaag
     5281 agactaagct acgtccgctt tattttgaag ataaatttcg gctgcccgga tacgttgttc
     5341 tatacgttcc aaaggtatac cggtttccgt cgccagttcc agtgccgtct tgccgccccg
     5401 gaattgttga tagagctgaa cagcgaaagg cctcatatcg gacccacctc cgttactcgg
     5461 caactgagtg aagcaatcag cgtgccaagc gttttgcatt gcagttcttg caacacactt
     5521 tatatatcgg gtgttttccc gatccctttg aataaagtgt taagcttcag tgctaccgat
     5581 tctgtttctt tttcgttttt ttgcaccagg gttcccgttg ttctcaaacg caacgcgttt
     5641 ttaactatct tctacctccc ggctgccagt caacagcgct agtctgcgac cagttctagt
     5701 acggttatct cgggcggaca atgatatcgc accggtaaca tcgagcagcc aacgccggcg
     5761 ctggtatagc cctgcaaact ccggtgatgc cacgcgccgc aggtatactc ccgcggacac
     5821 tttgcattca aaacgaccgg accgagccag gggaggcaaa tctgcccacc gtgcgtatgc
     5881 ccgcataggt acagttggat acccgccgac gctgcttcct cgtaaatttc cggactgtgg
     5941 gccagcaaaa tccgagtgct cccatccgga acctctttga gcgcctggct cagatcgtca
     6001 catccgtagt agtgcggatc gtcaaccccg gcaatccaga tcgaatcgac gcctttgcgc
     6061 agttctatcg cgtcgttgat caacatccgc acgccgattt tggagagatc caccgcaagg
     6121 tcggcgcagt cgtgattgcc gagaatcccg atgatcccgt aatgcgattg gaccctcgac
     6181 agcgccatcc gcatacgcgg aagaaccaga tcgtattcac cctccgtctc aaaacggtag
     6241 tctcccgtca ggacacatgc gtccaccgga tggcgcgata cggtttcggc gatgacctcc
     6301 gccaacccct ggactccatc gatgtggaag tcggacatgt gcaggattcg aaaatgcgaa
     6361 aagctggaag gaagctgcgg aatgcgtacg gtcgtgtggc ggactacggg ccgcaccgcg
     6421 ttgcggcagc cccggcgata taagccggcc gcctggagtg ccgtacgcaa gacggggccg
     6481 actaagcggt cgtgccggag gatgtaatct ccccgtccgt gggcgctccg tcctttgcgg
     6541 tggagttctt ccgccttgcg gcggcaggtt aatcgctgct ccaactcact ggccgggcgc
     6601 atgccgtgtt tgtttctccc cgtgatcggc gtagcctatc ccggtgccgg gcggcggtag
     6661 aaattgcaca gatccggacg gacctcgctg catctggtgc gtgtaaagca ctgaaagatt
     6721 accactgtat gagattggtc caccggttgc aaatttcaga atgggaggag agagagaatg
     6781 tatataccga tcagtttgct tctgctggtg ctgctgatta tcctgcttgt ctattatgtg
     6841 taggcgagcg aacacacagg gttacactcc gaccggttct gccgtttcga gggcggtgaa
     6901 ataagcacaa tcgggatgat ggaacaccag cgcgctgacg cttgcctcgg gctccatcat
     6961 catgccctcg gtgaggtgaa caccgatctc atcgggctgg aggagtttcc acactcccac
     7021 ttgatcgtcc aggttcggac aggcggggta tccgaagctg taccgcttcc cgcgatagcg
     7081 ggaggtgaaa cgctgttgca tggtcaggtc aacgggatcg ggaaagcccc agtcctcccg
     7141 gatgcgccga tgcagccact cggcggttgc ctccgcggtt tcaattccca gtgcctgtag
     7201 cgcatgagct ttgaagaact gcccctgctc cttcgccgct tcggaatgtt cccgaatgcc
     7261 ttcaccggcc gtcaccacga agagtgccag gtgatcgcgc tgcccgtctt taggatcgag
     7321 gatatagtcg ctgagacaga ggccgtcctc cttcgcctgc cttccaaatc gaaacacgtg
     7381 cacgggttcg ctgccgccgg gtgaaaacag cagaatggaa ttaccgtcgc gttcggcttc
     7441 gaagaactgc cacacggcac ggaccttcat gaagcttttg gcgtactcct tcacctcatc
     7501 cacatcgtga aacagtttca gcgccttctc gtcgcgttcc gccagagctt tttcgaaatt
     7561 gccccggaaa ccgagatgcc ggccgtaaag catgaacgga ttgatatagc tccagacctc
     7621 ggggagattg ggaatcgcgc gcatgcggcg gtccaggtac ggcactgccg gtatgggaat
     7681 ctcggtggcg actttcggac tgcgcccgtt tcccgaaggg accaaagccg gacttgccgt
     7741 ctctaccggg ggggccggct cttcccacgt atgagcggcc agcatgcccg tacgggtgtc
     7801 cgggttcata atttcgttca gcaggcgaag gccggtcatc gcatctttgg cgtagaaagt
     7861 gggtgcggaa tagctgggcg caatgcggga ttttgtaaat ctctccgaaa gcgcggctcc
     7921 gcccaccagc agcggcacgt tgatgccggc ttcatggaaa tcccccgccg tgatcaccat
     7981 ctgctgcgcg ctcttcacca ggagcccgga caggccgatc gcatccggct tgtgttcgtt
     8041 gtaggcgcgg attaattcat ctggtggaac cttgataccg agattgatga cccgaaaccc
     8101 gttgttggcg agaatgatct ccaccaggtt ctttccgatg tcgtgcacat cgccttttac
     8161 cgtggccagc acaatggaac ctcgcgtcga agtctcgctt ttttccatga agcgttccag
     8221 gtaggccacc gccgctttca tcgactccgc cgattgcagc acttcggcca cgatcagctc
     8281 gttattgttg aacagccggc ccacttcatt catgcccgcc atcaacggcc cgttgatgat
     8341 ctccagcgga gccgcgcctt ccgccctctt cgtatccaga tccgctatca gcccgtcctt
     8401 gctgccttcg acgatgtagt tggcgagccg ttcatccagc gagagcgcga ccgcggtctt
     8461 cttctgtttt ccggttgccg tccggaagtg ttcggcgatt gcggcaatgt ggaattgatt
     8521 gatcgcgatc ttttgttctc gcgattgctg ccgccagtcc tccggggcgg cgcggagtaa
     8581 attggcatca cgatgatccg ccggcacctc ccgcggcgga gtattgaaca gcaggtcctc
     8641 cgcaaggcgg cgttcttcgg ccggtatgga ggcgaagcgc tcgagctttt ccgagttgac
     8701 gatggctagg tccagaccag ccttggtcga gtagtagaga aaaaccgagt tcactacttc
     8761 gcgcgccgcg gggggcaacc caaaggagat attcgatacg ccgagaactg ttttcacaaa
     8821 cggcagggct tctttgatga ggcgtatcgc ttcaatcgtc tcgacggcgc cgccgatgta
     8881 attctcgtct ccggtagcac acgggaacac cagcggatcg atgatgatat cccccactgc
     8941 aagcccgtac ttttccgtca gcagccgcac cgagcgctcc gcaatcgcga gcttacgctc
     9001 acgcgtgaag gcctgggcct gcagtttgtc ctcgtcaata catcccacca ctacggcggc
     9061 accatacgct ttcgcgatgg ggcagaggcg ctcaaatttc tcctcgccgt cttccaggtt
     9121 gatggagttg atgatgctct tgccctggca gtacgtcagc gcgtgctcga cggcctttgc
     9181 gtctgtggtg tcaatcatca ccggggcttt gattttgcgg atcagttttt cgtagaagtg
     9241 cggaatatcg ttgatctcgt cgcgctcgct cgattgcagg catacatcga tcacctgggc
     9301 gccattgcga acctgccggc gcgcgatctc gctcgcttct tcccacttct cttcggcgat
     9361 caggttcttg aagagccgcg acccgatgac gttcgtcctc tcccctacca gcagcggcct
     9421 gtttgtttcc tccgcctcca ccagttcgat gcccgagtag tacgcacggt taggtgatgg
     9481 cttcacgatt cgcggcgctt tgccttccac catctggacg atggcgcgga tgtgttcggg
     9541 cgtggtgccg cagcagccgc cgatgatatt gagccatccg tggtcaacga agcgctccag
     9601 ttgcgccgca agagaagtag gcgtttccag atacttccca tcttcattcg gcaaaccggc
     9661 gttcgggtaa caggacaccc tggtggtggc catatcgttc aaggtgcgga tatggtcggt
     9721 catgaactcc gggccggtgg cgcaattgag gccgatggat agcagatcgg cgtgtgaaac
     9781 cgatacccag aaagcgtccg ccgtttgtcc ggcgagcatg gtgcccatcg gctcaatggt
     9841 gccggagacc atggcgggga ttttccgccc cagttcgcgt cccaggcgcc ctaccgccag
     9901 cagtgcggcc tttacgttgc gcgtgtcctg gcaggtctcg atcaaaagga catcggcgcc
     9961 cccgtcgatg agtcctttcg cctgctcatg gaacgtccgc tccaattcgg caaaggtgac
    10021 tcctcccgta accgtgatcg ctttggtggt aggccccatc gagccggcga cgaagcgcgg
    10081 tttgttcgga gtggagaact ccgctgccgc ctcacgggcc aggcgcgccg cctcgcggtt
    10141 gatctcgtag gcgtactgct cgagtccata ttcggccagt accaggctgg tggctc
//
LOCUS       DASTMK010000435        13489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_213369, whole genome shotgun sequence.
ACCESSION   DASTMK010000435 DASTMK010000000
VERSION     DASTMK010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213369"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFQ79_18630"
     CDS             <1..648
                     /locus_tag="VFQ79_18630"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEU0141745.1"
                     /translation="TFVSRILLILCLAVFPAAAATTDISKLLKGIEDRYNRARTLQIH
                     FAQTYTYAQGRRMETGDLYLRKPGKMRWVYSNPAGKIFVSDAKDAWFYSPAANRVEKM
                     KLKETDDMRAPMAFLLGRLDFSRDFKEFRSRPEGEAVFITAIPKSEKMPYREVSFLVS
                     PAHQIQRLIVTGQDGSVLQFDFSAEKVNPPLEDSLFGFQIPRGAEFVDLTNSQAN"
     gene            652..1860
                     /locus_tag="VFQ79_18635"
     CDS             652..1860
                     /locus_tag="VFQ79_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0141746.1"
                     /translation="MAEFTLKYADARGEIHQQVAEAGSEQELRERFSRQGYLVYSIKG
                     RSAAGLSAGLSRGRKKLNVEKFLIFNQQFVTLIRAGLPILKCLDLLADRLTDPKLGEH
                     VKAVRDDVKKGSMLSDAFAKRGVFPPIYVTSILAGEKSGALPEVLERFLTYQRLALAV
                     RKKILLSLMYPAILVLLVLCLIVFLVTYVVPSFAVLYGSMSAQLPAVTQLLIAVGTTA
                     RNYVIVGFVCLGGAIVAFRFWSRGNAAKARIDRVKLRTPVLGEIWIKYQVAQFSRVLS
                     TLLVGGIPLIQSLETASQSVGTVVLRNALETARKRVREGQSLSSSLEATGVFPELSID
                     MIEVGESTGALPSMLNSVAEFYEDDVNTRMTAALSLIEPAIMIFMGGFVAFVLVALYL
                     PIFSLADTLG"
     gene            1866..3488
                     /locus_tag="VFQ79_18640"
     CDS             1866..3488
                     /locus_tag="VFQ79_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU0141747.1"
                     /translation="MATDRLKLLDIEQEVAQARALAARYRCDFIDLREGKIDHDLFRS
                     IPVDLMFRYNFVPLGGHNGTLEIALADPRHLNLIDELSILLQKKLKVRVATLSQISDL
                     LKKTEQSQRVLEEVTEGFTLDVVGDEENQEETLSIDKLASSDSDIAPVIKLVDTTIFN
                     ALERRASDIHIETRDQEVAIKYRIDGVLHYAMPPIAKDWHSTIISRIKIMSELDIAER
                     RVPQDGRFRVRYKNRLIDFRVSIMPTIYGEDAVLRVLDKESMSEKFAKLSLDVVGFSD
                     DDLVKFRRYIKEPYGMVLVTGPTGSGKTTTLYAALSEIKNDEDKIITIEDPVEYQIKG
                     ITQIPVNEKKGLSFARGLRSILRHDPDKIMVGEIRDHETAQIAINAALTGHLVFTTVH
                     ANNVLDVLGRFLNMGVEPYNFVSALNCILAQRLVRVICEHCKTRVSYPDSYLSESGLD
                     PDEWRNVPLWEGQGCFECGGTGFRGRTAIHELLDLSERIREMILDKRPSSEIKRVSRD
                     EGMTFLRESAIEKARDGVTTLKEINKVTFIEG"
     gene            3507..4391
                     /locus_tag="VFQ79_18645"
     CDS             3507..4391
                     /locus_tag="VFQ79_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141748.1"
                     /translation="MRRLARIFEDPPPEYVFELSGAGIAYARHGHPPQTGFRRLEPGV
                     IAVSPLEDNVLNAEALVAGVKAAGGANGSRKRKPAALILPDYCARVTVLDFDDFPSES
                     GEQMSLVRFRTKKTVPFDIDSAAVSYEVQPKQGGRKTVEVVVAAVALEIIARYEAAFR
                     AAGYHPGFITTSALGVLRLLRGSGLTVAARWNGHTMTVSVQQGGALKLLRCVEFPEVG
                     IPEVLSMLHPTFAFIEDELGARPDKLFLCGFGDFTELFRAQCAEDLAVTVEPLKSRFG
                     TPGPDNAGLLGYLDSVEV"
     gene            4407..4967
                     /locus_tag="VFQ79_18650"
     CDS             4407..4967
                     /locus_tag="VFQ79_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141749.1"
                     /translation="MQLNLASEPFRRDRPMLVASGVVALLMVAVLGMLITLIVADREQ
                     SRDTRAEIERTTKRLAALTSEQNKLESILRQPNNAEVLERSVLLNMLLRRKGISWTLI
                     FSDLERIMPHNVRLINVRPQVTSGNTVLLQMVVGAQSAEPVRVMLINMEKSPLFGPAS
                     MQNWVPPSQNDPLYRYRVSVNYARKL"
     gene            4951..5568
                     /gene="pilO"
                     /locus_tag="VFQ79_18655"
     CDS             4951..5568
                     /gene="pilO"
                     /locus_tag="VFQ79_18655"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU0141750.1"
                     /translation="MPGSFNLTAVRRRLPALQGDFGRDPRFVVRLVLGLLLLANIVAA
                     LVLFKPWGGSAEELERRLADMDQQAVQKQASVVTLQNLVSKVEKARGEGDLFMKDYFL
                     SERSAYSTLVAELTEVAGKAKIKIKDHSFTEEPIEGAEHLKMLSISANYEGTYADLVE
                     FVNMLDRSPRLLIVENLTATPQQSGGALNINIKLDTFMRGEEVAP"
     gene            5565..6293
                     /locus_tag="VFQ79_18660"
     CDS             5565..6293
                     /locus_tag="VFQ79_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141751.1"
                     /translation="MKVGAERKKVILLVGLLAFAVVLYLYNSRSEFEEGSAQAPVPSA
                     SQVQIPEAPRAGSNTPATSALISQAPRIGSSSSRVSSEEFRPSLKKLKAEGAIDPMTL
                     DPTLHLDLLAKLQDVNIEGGERSLFEFAQPPAPKTPEPKVVPKPLNAAAEEKKGEGKN
                     EAASKPPPPPIPLKFYGYAAPVKQGVKRAFFLDGDDIFVASEGEIVKKRYKIVRIGVN
                     SAVVEDTQHENNQQTLPLVQEMAG"
     gene            6290..8065
                     /locus_tag="VFQ79_18665"
     CDS             6290..8065
                     /locus_tag="VFQ79_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141752.1"
                     /translation="MSETRRDRERGFALLLIFVLAAGIAISLYMQLPRVAFEMQREKE
                     EKLIERGEQYKRAIQIFVRKNKRYPNSMEELEGRAGEVHFLRRKYIDPMTGKDEWRLI
                     HVGPNGQLLDSVVQPSEEEKKKQEGQSENTFISELPTVGATGQEGQSGGNPFLRQRDS
                     DKPGAPGSTGAPVQSAPEMAGQPYSPQAGGYPGAPQQSNPYQQQTNPYQQPDPNQQPY
                     ANPAGPPPNPGYAQANQPNPFFGQTGVPAQGGQTGVQQPGQPQVVPGQPGQQPFGQQQ
                     MQVQQGYQQPGMVAPQAPNTPQGQQPALPSFLLPRPQTQGSLPGGSLPGGSIPSGQVG
                     GNAQQQQFQQQQYQQPQQYPQQQYVQQQQYPQQQAYQQQLQQQQQQQQQSRQQPSPQQ
                     LYQQQYQQYQQQYQQPQPYQPPAAQQPSYMQRRFQQLPYEQQQQLQQQQWQQQQQQQQ
                     QQQQQQQPRTSSGSYPVTPAGQQNPALQAIQQQLTRPRPGGLAGLQGSATSGGQTLGG
                     GIAGVASKYESEGIKIYRERTKYNEWEFIYDPKEDKTMVGSAAGQQQQQQGQGRTPGS
                     LSGSPSGSSPQSPMPGPNRMGPGTR"
     gene            complement(8062..8286)
                     /locus_tag="VFQ79_18670"
     CDS             complement(8062..8286)
                     /locus_tag="VFQ79_18670"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEU0141753.1"
                     /translation="MPTRGGARKRETTTAAVERVPEAFQAINESSPQGEIAQIAYRLY
                     EERTRGGNSGSAEEDWLKAEEEFRRIRGRG"
     gene            complement(8311..10251)
                     /locus_tag="VFQ79_18675"
     CDS             complement(8311..10251)
                     /locus_tag="VFQ79_18675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0141754.1"
                     /translation="MRREVLIVLAFVLLLRLPFLNQAIQGDDVYYIAAAEHAQIDPLH
                     PGHARFVFLGDEVSMQGHPHPPLNSWVLAILLAVLGDIREVPYHAAYILFSLLAAFSM
                     WLLARRFSPQPLWATLLFLVTPAFVVNGNSLESDVPFLAFWLAAVALFVRAVDRRSGA
                     WLGASALAMALAAMTAFQAVVLAPVLAVYVWLKRRTWMAGWAVTLTAPATIAAWQLYE
                     RLTSGALPAAELAGNFRTYNLQNLAAKFNNAVALTAHLGWIVFPLAALLAFGRFRKPW
                     ILAAGAVAGAIYLDSHPLFWVSFGTGVVILIGLLHRPLHANADTRFLSAWPLIFFLSA
                     LVLFFAGSARYLFPMAAPLALLVSRAVSVRWLATGFALQAALSFSLALVNYQHWDGYR
                     RFAASLRDQTTSARTWINGEWGLRFYFESDGGLAVRKGQAVRPGEILISSELAYPVPV
                     TTGGGELTTMAGEEIRPALPFCLIGLDCRSAYSTVAAGNFRPFDIAHRPVDRVRAAMV
                     MERKPTLENLLMSAAEADSHIVSGVFQLEEGRYRWIGGRAVFVLKSPAVSSPLRISFF
                     VPGGAPARRMSAYVDGKLVAETSFTGPGSHELVSPPVMPEGSTATLAVEVDRTFQPPN
                     DRRELSVILVEAGFQRNISSGR"
     gene            complement(10262..11764)
                     /gene="gcvPB"
                     /locus_tag="VFQ79_18680"
     CDS             complement(10262..11764)
                     /gene="gcvPB"
                     /locus_tag="VFQ79_18680"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEU0141755.1"
                     /translation="MRSKARQHISQNEPLLFERSSPGKKAYQLPQLDVPAVDAVKTLG
                     EEHVREEIEGFPEVSEVEAIRHFTRLSTWNYAIDHGMYPLGSCTMKYNPRINELVARL
                     DGLAWAHPYQPEALSQGAMRIIAVLEAALAEITGMDAVTLQPAAGAHGELTGILVIRA
                     LLESRGNPRRKLLIPDSAHGTNPATASMAGYQVENLKSNDRGMVDVEALAAAVNEDVA
                     ALMITNPNTLGVFEQNITRISEILHAKGALVYMDGANMNALVGITRPGDFGMDVMHLN
                     LHKTFSTPHGGGGPGSGPVAVKKELEPFLPGPRLKRSAGAWSWNYDLPQSIGRVRAFY
                     GNFGVLVRALAYIMAHGGDGLRRATMDAVLNAIYIRKSLDRYYGLPLDAPSMHEVVFS
                     DEKQARHGVRTGDLAKRLIDYGFHPYTVSFPMIVKGALMIEPTETEGKIELDAFIDAM
                     IAIAREVEEDPEMVLKAPYSTRTSRVDEVSAARKPVLRWRPQPPAGKAAD"
     gene            complement(11844..13157)
                     /gene="gcvPA"
                     /locus_tag="VFQ79_18685"
     CDS             complement(11844..13157)
                     /gene="gcvPA"
                     /locus_tag="VFQ79_18685"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEU0141756.1"
                     /translation="MRYLPKSEIERREMLSVCGVSSIEELFDSLPPEVRLNRPLKLDP
                     GKSEYEIVDYFRERAVENANGYASFLGAGVYNHYRPVLVDTVVSRGEFLTSYTPYQAE
                     ISQGTLTAIFEFQTMICQLTGMEVANASMYDGSTAVPEAAMMAARITGKDRILAARSV
                     HPEYREVLHTYAKHQGLPVVEFAYDAESGEVDSSALEAGLDEHTAAVIVQTPNFFGVL
                     ENIERLAGIAHGRGALLVVVFTEAVSLGLLKPPGAADIVAGELQSFAISPGYGGPYAG
                     VIATKEQYMRQLPGRLVGETLDSHGNRAYCLTLSTREQHIRREKATSNICTNQALIAL
                     MATVFMTVYGKQGLRELAEQNLAKAHYLASGLSVRFSGRFFNEFVVKTNGRSPEEINQ
                     RLLDHRILGGLPLGRFYPELNDTLLLCATEMSRRESMDRVKEALT"
     gene            complement(13188..>13489)
                     /gene="gcvH"
                     /locus_tag="VFQ79_18690"
     CDS             complement(13188..>13489)
                     /gene="gcvH"
                     /locus_tag="VFQ79_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU0141757.1"
                     /translation="TDHAQKELGDIVYVDLPKAGASVQAGKTLGSVESVKAVSDIYSP
                     LTGEVAEINELLSSAPEKLNEDPHGTAWLVKLKLNAPEEAGQLMTAADYQAYIGE"
BASE COUNT         2856 a         4047 c         3993 g         2593 t
ORIGIN      
        1 acatttgtga gccgcattct gcttatattg tgcctggcgg tgtttcctgc cgccgctgcc
       61 acaaccgata tctctaagct gctcaaagga atagaagacc gctacaatcg cgcccgcacg
      121 cttcagatcc attttgcgca aacctatacg tatgcccagg gccgccggat ggaaaccggg
      181 gacctttacc tgcgcaagcc gggaaaaatg cggtgggtgt atagcaatcc ggcagggaag
      241 attttcgttt ccgacgccaa ggatgcctgg ttctacagcc cagccgcgaa ccgggtagaa
      301 aagatgaagc tgaaggaaac cgatgatatg cgggcgccga tggccttcct gctcggcagg
      361 ctcgatttct cccgggattt caaagagttc cgctcccggc ccgaaggcga ggccgtgttt
      421 atcacggcga ttccgaaatc ggagaagatg ccctatcggg aagtgagttt cctggtttcg
      481 ccggcgcacc agattcaacg ccttattgtt accgggcagg acggttctgt gctgcaattc
      541 gatttttccg ccgagaaggt gaacccgcct ctcgaagaca gtctttttgg ctttcagatc
      601 ccgaggggtg ccgagtttgt cgatctgaca aactcgcagg caaattgaac cgtggctgaa
      661 ttcaccctca agtatgcaga cgcccgcggc gagatccacc aacaggtggc cgaggcgggg
      721 tccgagcagg aattgaggga gcgcttctcc cgccagggct acctggtgta ttcgatcaag
      781 ggccgttcgg cggcggggct ttccgccggc ctgagccgcg gccggaagaa gctgaatgtt
      841 gaaaagttcc tgatcttcaa ccagcaattt gtcacgctga ttcgcgctgg gttgccgatt
      901 ttgaagtgcc tggatcttct ggccgaccgg ctgacggacc cgaaattggg cgagcacgtg
      961 aaagcggtcc gcgacgacgt gaagaagggc tccatgttga gcgatgcgtt cgctaaacgc
     1021 ggtgtctttc cacccatcta cgtcacctcc attttggcgg gggagaaaag cggagcgctg
     1081 cccgaagttc tcgagcgctt tctgacctac cagcggctcg cgctggcggt gcgcaaaaaa
     1141 attctgctgt cgctgatgta tccggcgatt ctggttctcc tcgtactgtg cctcatcgtc
     1201 tttctggtta cctacgttgt ccccagcttt gcggtgcttt acggcagcat gtcggctcag
     1261 ttgccggcgg tcacccaatt gcttatcgcg gtgggaacga ccgccagaaa ttacgtgatt
     1321 gtgggattcg tgtgccttgg cggcgcgatt gtggccttcc gtttctggtc ccgaggcaac
     1381 gccgccaagg cgcgcatcga tagggttaaa cttcgaacgc cggtgctggg cgagatctgg
     1441 atcaaatacc aggtggcaca attctcgcgc gtgctcagca cgctgctggt gggcggcatt
     1501 ccactcatcc agtcgcttga aaccgcttcg cagtcggtcg gcaccgtggt gctgcggaac
     1561 gctctcgaaa cggcccggaa acgggttcgg gaagggcaat cgctttcttc gtctctcgag
     1621 gcaacgggtg tttttccgga actctccatc gatatgattg aagtagggga gtccactggc
     1681 gcgttgccgt ccatgctgaa cagcgtggcg gaattctacg aggacgatgt aaatacccgc
     1741 atgacggcgg cattgtcgtt gatcgagccc gccatcatga ttttcatggg cggatttgtg
     1801 gcgttcgtgc tggtagctct ctacctgccg atcttctcac tggctgacac gttggggtaa
     1861 gtccgatggc aaccgaccga ttgaaattgt tggatatcga acaggaggtg gcacaggcgc
     1921 gcgcactggc tgcccgctac cgttgcgact ttatcgacct gcgcgaaggc aagatcgatc
     1981 acgacctgtt ccggtccatt ccggtcgacc tcatgttccg gtataacttc gtaccgttgg
     2041 gaggacacaa tggcacgctc gaaatcgctc tcgccgaccc tcgccacttg aatctgatag
     2101 acgagctctc cattctgctt cagaaaaaac tgaaagtgag agtggcgacg ctgtcccaga
     2161 tctccgacct gctcaagaaa accgaacagt cgcagcgcgt actcgaagag gtgaccgaag
     2221 ggttcacgct ggacgtggtc ggcgacgagg agaaccagga agaaacgctc tccatcgaca
     2281 agctggcctc gtcggacagc gacatcgcac cggtcatcaa gctggtggac accaccatct
     2341 tcaacgcgct cgaacggcgc gccagtgaca tccacatcga gacgcgcgac caggaagtcg
     2401 ccatcaagta ccgtatcgac ggggtgctgc actacgcgat gccgcccatc gcgaaggatt
     2461 ggcattcgac catcatttcg cgtatcaaga tcatgtccga gctggacatc gccgaacggc
     2521 gcgttccgca ggacggccgt ttccgggtcc ggtacaagaa ccgtctcatc gatttccgcg
     2581 tctcgatcat gccgaccatt tacggagagg atgcggtgct ccgtgtcctc gacaaggagt
     2641 cgatgagcga gaagttcgcc aagctctcgc tcgatgtggt gggcttttcg gatgacgacc
     2701 tggtgaaatt ccgccggtat atcaaagagc cttacggaat ggtgctggtt accggtccga
     2761 cgggatcggg caagaccacc acgctctatg ccgcgctgag cgaaatcaag aacgacgaag
     2821 acaagatcat tacgattgag gatccggtcg aataccagat caaaggcatc acgcagattc
     2881 cggtgaacga aaagaagggc ctcagcttcg ctcgcggtct gaggtccatt ctgcggcacg
     2941 atcccgacaa gatcatggtc ggcgagatcc gcgaccacga gacggcccag atcgccatca
     3001 acgccgcgct tacgggacac ctcgtgttca ccaccgttca cgccaacaac gtgctggatg
     3061 tgctggggcg cttcctcaac atgggagtcg agccgtacaa tttcgtctcg gcgttgaatt
     3121 gcattctggc gcagcggctg gttcgagtca tctgcgaaca ttgcaagacg cgggtcagct
     3181 acccggattc gtatctctcg gagagcggcc tcgatcctga cgagtggcgg aatgtgcctt
     3241 tgtgggaagg ccagggatgt tttgaatgcg gcggcacggg gtttcgcgga agaaccgcca
     3301 tccacgaact tctggatctt agcgagcgga ttcgcgaaat gattctcgac aaaagaccca
     3361 gttccgaaat caagagggtc tcccgcgacg aaggcatgac cttcctgcgg gaatccgcca
     3421 tagaaaaagc ccgtgatgga gtaactacgc tgaaggagat caacaaggtc accttcatag
     3481 aaggttgatg cgcggatatg ttgctgttga gacgactggc tcggatattc gaagacccgc
     3541 cgccggagta cgttttcgaa ctgtcgggcg ccggcatcgc ctacgcccgg catggacatc
     3601 cgccgcagac cggcttccgc cgcctcgagc ctggagtgat cgcggtctca cctctcgagg
     3661 acaacgttct gaatgccgag gcgctggtag ccggcgttaa agccgccgga ggtgcgaacg
     3721 gctccaggaa gcgcaaaccg gctgccctga tactgccgga ttactgcgcg cgcgtcaccg
     3781 tgctcgactt tgacgatttt ccttctgaat ccggtgagca gatgtctctg gttcgcttcc
     3841 gtacgaagaa gaccgtgccc ttcgacatcg attccgcggc cgtcagctac gaggtacaac
     3901 cgaagcaggg cgggcggaag acggtggaag tggtggtggc ggcggtagcg ctcgagatca
     3961 tcgcacgcta tgaagccgcg tttcgcgcag ccggatatca cccgggattc atcaccacgt
     4021 cggcgctcgg cgtgctgcgc ttgctgcgcg gatctggtct caccgtcgca gcccgctgga
     4081 atgggcatac gatgacggtt tcggtgcagc agggaggtgc gttgaagctg ttgcggtgcg
     4141 tcgagttccc ggaggtgggg attccggaag tgctctcaat gctgcatccc acttttgctt
     4201 tcattgagga tgaactcggg gcgcggccgg acaaactgtt cctgtgcggc ttcggcgatt
     4261 tcaccgagct tttcagggcg caatgcgcgg aggatctggc tgttacagtg gagcccctga
     4321 agtcccggtt tgggacgccg ggaccggata atgcaggctt attgggatac ttggattccg
     4381 tggaggtata gggcttgtcg cagaggatgc aattaaattt agcgagtgag ccgttccggc
     4441 gcgacaggcc gatgctggtg gcttccggtg tggttgcgct tctgatggtg gctgtcctgg
     4501 gcatgttgat cacactgatc gtcgccgacc gcgaacaatc gcgcgacacc cgggccgaga
     4561 tcgaacgtac cacgaaacgg ctggcggcgc tcacttccga acaaaacaaa ctcgaatcca
     4621 ttctgcgcca accgaacaac gccgaggtgc tggagcgcag cgtgctgctg aacatgctgc
     4681 tgcggcgcaa gggcatcagt tggacgctga tcttctccga cctggagcga atcatgccgc
     4741 acaatgtccg gctgatcaat gtgcggccgc aagtgacatc gggcaacacg gtactgctcc
     4801 agatggtggt gggagcgcag tcggccgagc ctgttcgggt aatgctgatc aacatggaga
     4861 agtcgccgct cttcggcccc gcatccatgc agaactgggt accgccttcg caaaacgatc
     4921 cgctgtaccg ctacagagtg agcgtcaatt atgcccggaa gctttaatct gaccgcagtt
     4981 cgcagacgcc ttcccgcgct tcagggcgac ttcgggcgtg acccgcgatt cgtggtacgc
     5041 ctggttcttg gactgttgct gctcgccaat attgttgcgg cgctggtgct cttcaaaccc
     5101 tggggcggat cggcggaaga actggaacgc aggcttgccg atatggacca gcaggcggta
     5161 cagaaacaag ccagtgtagt gacgcttcag aacctggtga gcaaagtaga gaaggcgcga
     5221 ggagaaggcg atttgttcat gaaggactac ttcctgtcgg aacgcagcgc ctattcgacg
     5281 ttggtagccg aactgacaga agttgccggg aaagcgaaaa tcaagatcaa ggaccactct
     5341 tttacggagg agccgattga aggagccgag catttgaaaa tgttgagcat ctcggctaac
     5401 tacgaaggaa cgtatgcgga ccttgtcgag ttcgtcaata tgctcgaccg ctcgccgcgg
     5461 ctcctcatcg tcgaaaacct gacggccacg ccgcagcagt ccggcggtgc gctgaatatc
     5521 aacatcaagc tggacacgtt tatgcgcggt gaggaggtcg cgccatgaaa gtaggggcgg
     5581 agcgaaagaa ggtcattttg ctggtaggcc tgctggcttt cgcggtcgtg ttgtatcttt
     5641 ataactcccg ttctgagttc gaagagggct ccgcgcaggc tcccgttcca tcggcgtccc
     5701 aggtccagat acccgaagcc cctcgtgcgg gttcgaatac ccctgccacc tccgctctga
     5761 tttcgcaggc gccccgtatc ggaagcagtt ccagccgcgt gtcctcggag gagttccggc
     5821 cctctctcaa gaagctcaaa gcggaaggtg cgatcgatcc gatgacactc gatcccactt
     5881 tgcatctcga tctgctggcg aaactgcagg atgtgaatat cgaaggaggc gagcgaagcc
     5941 tgttcgagtt cgcgcagccg cccgctccca agacacccga acccaaagtg gttcctaaac
     6001 cgctgaacgc cgcagcggaa gagaagaaag gcgaagggaa gaacgaagcc gcgtcgaagc
     6061 cgccaccccc gcctattcca ttgaagttct acgggtatgc ggccccggtg aaacagggag
     6121 tcaaacgggc tttcttcctg gacggcgatg acatcttcgt agcatccgaa ggcgaaatcg
     6181 tgaagaagcg atacaagatc gtgcgcatcg gtgtgaattc ggcggtggtt gaggacacgc
     6241 agcacgagaa taaccagcag acgctgccgc tggtgcagga gatggctgga tgagcgaaac
     6301 gagacgcgac cgggagcggg gattcgcgct gctcctgatc tttgttctgg ccgccggcat
     6361 tgctatctcg ctttacatgc agttgccgcg tgtggctttc gagatgcagc gcgaaaagga
     6421 agagaagctc atcgaacgcg gcgagcaata caagcgcgcc attcagatat tcgtgcgaaa
     6481 gaacaagcgc tatcccaaca gcatggaaga actcgaggga cgggccggag aagtacactt
     6541 cctgcggcgc aagtatatcg atccgatgac agggaaggac gaatggcgcc ttattcatgt
     6601 cggtcccaac ggccagttgt tggattcggt ggttcaacca tccgaagaag aaaagaagaa
     6661 gcaggagggg cagtcggaga acacattcat ttctgagctc cctacggtag gtgccaccgg
     6721 gcaggaaggc caatccggcg gcaatccttt cctgcgccag cgcgacagcg acaaacctgg
     6781 cgcacctggc agcacgggcg cgcccgtcca gagtgctccg gagatggctg gacaaccgta
     6841 ttctcctcag gcgggcggat atccaggcgc gccgcagcaa tccaatccat accagcagca
     6901 gactaatcca taccaacaac cggatccgaa ccaacaaccg tatgcgaatc cggccgggcc
     6961 gccgcccaat cccggttatg ctcaggcgaa ccagccgaac ccgttcttcg ggcagacagg
     7021 agtgcctgct cagggcggcc agacgggtgt gcaacagcct gggcaacccc aggttgttcc
     7081 aggccaaccc ggccagcagc cgttcgggca acagcagatg caagtgcaac agggctatca
     7141 gcaaccgggc atggtagcac cgcaggcccc caatactccg cagggacagc agcccgcact
     7201 gccgtcattc ctgctcccca ggccacaaac ccagggcagc ctgccgggcg gcagcttgcc
     7261 cggcggcagc ataccttccg gacaagtggg cggcaatgcg cagcagcagc agtttcaaca
     7321 acagcagtac cagcagccgc agcagtaccc ccaacagcag tatgtgcaac aacagcagta
     7381 tccgcagcag caggcatatc agcagcaatt acaacaacag cagcagcaac agcaacaatc
     7441 ccggcagcaa cccagcccac agcaactgta ccagcagcaa taccagcagt atcaacagca
     7501 gtatcaacag ccgcaaccgt atcagcctcc ggctgcacag cagccttcct acatgcagcg
     7561 gcgctttcag cagcttcctt acgagcaaca gcagcagttg cagcagcaac agtggcaaca
     7621 gcagcaacag cagcaacaac agcagcaaca acagcaacaa ccccggacgt cctccggttc
     7681 ctacccggtg acacccgcgg gacagcagaa tccagcgttg caggcaattc aacagcagtt
     7741 gacaagaccg cggccgggag gcctcgcggg tctgcaaggc agtgcaacca gcggcggaca
     7801 gacactgggc ggggggatcg ctggtgttgc cagcaagtat gaatcggaag gaatcaaaat
     7861 ctaccgggag cggactaaat acaacgagtg ggaattcatc tacgatccga aggaagacaa
     7921 aaccatggtg ggaagtgcag ccggccagca gcagcaacag cagggccagg gccggacgcc
     7981 gggttcgcta tcgggctctc catcgggtag cagcccgcag tctcctatgc ccggcccgaa
     8041 ccgcatgggc ccggggacgc gctagcctct tcctcttatc cggcgaaatt cctcttccgc
     8101 tttcaaccag tcctcttcgg ccgatccgga atttccgccc cgcgtgcgtt cttcataaag
     8161 ccggtaggca atctgtgcga tttcgccctg agggctggac tcattaatgg cctggaatgc
     8221 ttccggaacc cgctccacgg cagccgtggt tgtctctcgt ttccttgcgc ccccgcgtgt
     8281 cggcatgcgt aaaatcctct gtggatgaga ctaccggccg gaagaaatgt ttctctggaa
     8341 cccggcctcc accaggatca cgctgagctc gcggcggtcg ttcggaggct gaaaggtacg
     8401 gtctacctcc accgccaagg tagcggtgga tccttccggc atcacgggcg gtgaaaccaa
     8461 ttcatggcta cccggtccgg tgaaactggt ctcggcgacg agcttgccgt ccacgtaggc
     8521 gctcatacgc cgtgcgggtg ctccgccggg aacgaaaaac gaaatgcgca gcggggatga
     8581 taccgctgga gatttcagaa cgaagaccgc cctgcctccg atccagcgat agcggccctc
     8641 ttcaagctgg aagaccccgc tcacgatgtg gctgtcggcc tccgccgcgc tcatgaggag
     8701 gttctcgagt gtgggcttcc gctccatcac catggcggcg cgcacccggt cgacaggccg
     8761 gtgtgcaatg tcaaaggggc ggaagtttcc ggccgccacg gtcgagtatg cggagcggca
     8821 gtccaacccg atgaggcaga acggcaatgc cggccggatc tcctcgccgg ccatcgtggt
     8881 gagttcgccg cctcccgtcg tgaccggaac gggataagcg agttcactcg aaatcaggat
     8941 ctcgccgggc cggactgctt gccccttgcg aaccgcaagc ccgccgtcac tttcaaaata
     9001 gaaccgcagc ccccattcgc cgttgatcca cgtgcgtgcg cttgttgtct ggtcccttag
     9061 cgaggccgcg aagcgccggt atccatccca gtgctgataa ttcaccaggg ccagcgagaa
     9121 gcttaacgcg gcctgtaacg cgaaacctgt cgcgagccat cgcacgctca ccgcacggct
     9181 caccagcaaa gccagcggcg cagccatggg aaacagatat cgcgccgagc ccgcaaagaa
     9241 caggaccagc gcggacagga aaaagatcag cggccacgcc gagaggaacc gcgtatcggc
     9301 attcgcatgc aggggccggt gcaacagccc gattagaatt acgacgcccg ttccgaaaga
     9361 aacccagaac agcgggtgtg aatccaggta tatggcaccg gccaccgcac cggcggcgag
     9421 gatccacggt ttccggaaac gcccgaacgc cagtagtgcc gccagcggaa aaacgatcca
     9481 gccgaggtgt gccgtcagcg ccaccgcgtt gttgaatttc gctgcgaggt tttgcaggtt
     9541 gtaagtgcgg aaatttcccg ccaattccgc cgcgggcaat gcacccgaag taagacgttc
     9601 atacaactgc caggccgcga tcgtcgccgg cgccgtcaaa gttaccgccc atccggccat
     9661 ccaggttcgc cgtttcagcc acacgtagac ggcgagaacc ggagctaaca cgactgcctg
     9721 aaaagccgtc atggcggcca gcgccatcgc cagggccgat gcacccaacc acgcgccgga
     9781 gcgtctgtcc accgcgcgaa cgaacaacgc caccgcggcg agccagaacg ccaaaaacgg
     9841 aacatccgat tcgagcgagt tgccgttgac aacaaaagcg ggcgtaacca aaaagagcag
     9901 cgtggcccac aagggctgtg gtgagaacct ccgggcgagc agccacatcg aaaacgcggc
     9961 aagcagcgag aaaagaatgt aagcggcgtg atacggaacc tcgcggatat cgccgagcac
    10021 cgccagcaga atcgccagca cccaggagtt cagaggcgga tgcgggtgac cctgcatcga
    10081 gacttcatca cccagaaaga cgaagcgtgc atggccggga tgcagcggat cgatctgcgc
    10141 gtgttcggcc gccgcgatgt agtaaacgtc gtctccctga atcgcctggt tcaaaaacgg
    10201 cagacgcaat agcagaacga atgcgagaac gatgagtact tcgcgccgca tgaggtccgc
    10261 cttaatcggc ggctttgccg gcgggtggct gtggcctcca gcggagtacg ggctttcggg
    10321 cagccgagac ctcgtccacc cggctcgttc gcgtgctata cggcgctttc agcaccattt
    10381 cgggatcctc ttccacttcg cgcgcgatgg cgatcatggc gtcgatgaag gcgtcgagct
    10441 cgatcttgcc ttcggtttcg gtcggctcaa tcatgagcgc acccttcaca atcatcggaa
    10501 agcttacggt gtaggggtga aatccataat cgatcagccg cttggccagg tctcctgtgc
    10561 ggacgccgtg ccgggcctgc ttttcatcgc tgaataccac ttcgtgcatg ctcggagcgt
    10621 ccagcggaag gccgtagtaa cggtcgagac ttttgcgaat gtagatggca ttcaggacgg
    10681 cgtccatggt ggcccgccgc agcccgtcgc cgccgtgcgc catgatgtaa gccaacgcac
    10741 gcaccaggac gccaaagttt ccataaaacg cgcgcacccg cccgatggac tgcggcagat
    10801 cgtaattcca gctccaggcg cccgcggaac gtttcaggcg aggccccggc agaaacggtt
    10861 ccaactcctt tttcaccgcc accggacccg aacccggccc gcccccgccg tgcggtgtcg
    10921 agaatgtctt atgcaggttc aggtgcatca cgtccatgcc gaaatcgccg ggccgcgtga
    10981 tgcccaccag tgcgttcatg ttcgcgccgt ccatgtacac cagcgcgccc tttgcgtgca
    11041 gaatctccga aatgcgcgtg atgttctgct cgaatacgcc cagcgtattc gggttggtga
    11101 tcatcagcgc cgcgacgtct tcattcacgg cagccgccag cgcttccacg tccaccatgc
    11161 cgcggtcgtt cgacttgaga ttctccacct ggtaaccggc catggatgcc gtagccggat
    11221 tagtgccgtg cgccgaatcg ggaatcagga gctttctgcg cgggttgccc ctgctttcga
    11281 gcagcgcccg tatcaccaga atgccggtca gctctccgtg cgcccccgcc gcgggttgca
    11341 gcgtgactgc gtccattccg gtgatctccg ccagcgccgc ttcgaggacc gcgataatcc
    11401 tcatcgctcc ctgggacagc gcctcgggtt gatacggatg cgcccatgcc agaccatcca
    11461 gccgcgctac aagctcgttg atccgcgggt tgtacttcat cgtgcatgag ccgagcgggt
    11521 acatgccgtg gtcgatagcg tagttccacg tcgacaggcg cgtaaagtgc cggatggctt
    11581 ctacttcgct tacctcggga aagccctcga tctcttcgcg aacatgttcc tcacccaatg
    11641 ttttaacggc atcgacggcg gggacatcca gttggggaag ctgatatgct ttcttgcccg
    11701 gcgagctgcg ctcaaacagc agcggttcgt tctggctgat gtgttgtctg gctttcgatc
    11761 gcatctattg tcctgtcaac tgctccgtga ccgtcgcggc tcagaaacaa aggtgttacg
    11821 gagccgcgcg cgtgagcaag cggttatgtc aacgcttcct tcacccggtc catgctctcc
    11881 cgcctgctca tctcggtggc gcataacagc agcgtatcgt tcaactccgg gtaaaagcgg
    11941 ccgagaggca atccgccgag gatgcgatga tccagcagcc gctgattgat ctcttccggg
    12001 gaacgtccat tcgtctttac cacgaactca ttgaaaaatc gcccggagaa gcgcacgctc
    12061 aggccggagg ccagatagtg cgccttggcc aggttctgtt cggcgagctc ccgcaaaccc
    12121 tgcttcccgt acacggtcat gaagaccgtg gccatcagcg cgatcagagc ctggttcgta
    12181 cagatgttcg aggtcgcttt ttcccgccgg atatgctgtt cgcgcgtgga aagcgtaaga
    12241 cagtaggcgc ggtttccgtg cgaatccagc gtttccccca ccagcctgcc gggcagttgc
    12301 ctcatgtatt gttctttggt ggcaatgacg cccgcgtatg ggccgccgta gcccggcgaa
    12361 atcgcgaacg actgcaattc gccggcgacg atatccgctg cccccggcgg tttcaaaagt
    12421 ccgagcgaca ccgcttccgt gaagacgacg accagaagag cgccccggcc gtgggcgatg
    12481 ccggccagcc gctcgatatt ctccaacacc ccgaaaaaat tgggtgtctg cacgatcacc
    12541 gcagcagtgt gttcgtcgag ccccgcttcg agcgcggaac tgtccacttc acccgactcc
    12601 gcgtcatagg cgaactccac aacgggcagc ccctggtgct tcgcataagt gtgcagcacc
    12661 tcgcggtatt cagggtgaac ggaccgggca gcgagaatac ggtcctttcc ggtaatccgc
    12721 gccgccatca tcgccgcttc cggcaccgcg gtggagccgt catacatgga cgcgttcgcc
    12781 acctccatgc cggtgagctg gcagatcatc gtttggaatt cgaagatcgc cgtcaacgtc
    12841 ccctgtgaaa tctcggcctg gtacggcgtg tacgaagtca ggaactcccc gcgcgagacc
    12901 accgtatcca ccagcaccgg acggtaatgg ttgtacacgc ccgcacccag aaacgacgcg
    12961 tagccgtttg cgttctccac ggcgcgttcg cggaaataat ccacgatctc gtactccgac
    13021 ttgccggggt cgagtttcag cggacggttc aagcggactt cgggcggcag cgaatcaaac
    13081 aactcctcaa tcgaggacac gccgcaaacg gaaagcattt cccggcgttc tatttcagac
    13141 ttcggtaaat agcgcatcag tagcggacgg gcggggaaac ttctatctta ctcccctatg
    13201 taggcctgat agtcggcagc ggtcataagc tgcccggctt cttctggcgc gttcagcttc
    13261 agcttgacga gccacgccgt tccatgcggg tcttcgttga gcttttcggg agcgcttgac
    13321 agcagttcgt tgatctcggc cacctctccg gtcaggggcg aatagatatc ggataccgcc
    13381 ttgacggatt ccaccgatcc cagcgttttt cccgcttgca cggacgcgcc ggctttcggc
    13441 aggtccacat agacgatgtc accaagctct ttctgtgcgt ggtcggtaa
//
LOCUS       DASTMK010000436         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_246296, whole genome shotgun sequence.
ACCESSION   DASTMK010000436 DASTMK010000000
VERSION     DASTMK010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246296"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1988
                     /locus_tag="VFQ79_18695"
     CDS             300..1988
                     /locus_tag="VFQ79_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141758.1"
                     /translation="MHAQKFLPDMTITRRNLMLPAAAAVFAQTGEPSWLRQVIDRHDS
                     HVEHLLKQQITSPRDRWRGIFPDEYGLYFPAAAAGIIDAMAAAYLHPGSKFHKNAELP
                     RRIQLAADLLAREQTSDGNWDLPITNFNSPPDTGFILRNLCPPAVLLKRAQETELFGQ
                     LEPLLRKAGGGISKGGVHTPNHRWVICAALSQLYELFEEPGYLKRIDQWLAEGIDIDP
                     DGQFSERSTYVYNPVTDNALTTMAAKLNRPHLLEPVRNNLESMLYLLHPEHEVVTEIS
                     RRQDRNQRGDMGPYWFSLAYLARHHDNRDGRFETLAAHFAPTRASLSALMEYPELAAP
                     GPALVSVPDHYRRAFPHNQLFHIRRGRMSAVVLAGGRSRFFTLRNGNAVVNAVRFASA
                     FFGKGQFSAPVIEEAGNTFRMTQRLEAPYYQPLDPPERVNAGQWEAVRPKRKKSEICR
                     LTQSVTISEHGTGFRLRIRSEGTKDVPLAVEINLREGGKLESVVPAQKVTEGWIFESG
                     HAIYRAGPDAIRFGPGTRAHVYTQVRGAEPKLPGPSVYLTGTTPFDHTIAFECL"
     gene            1998..3284
                     /locus_tag="VFQ79_18700"
     CDS             1998..3284
                     /locus_tag="VFQ79_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0141759.1"
                     /translation="MNRRTFFRSAAGAGALFQVDAVRRAQAAVQSISGRPADLVARDE
                     DFWLNIRHAFTVDRNLINLNNGGVSPSPRIVMDTEKRYLEIENMTPSYYMWHVLDPGI
                     ETVRRRLAREFGCDSEEIAITRNASEALEIVQLGLDLKPGDEILTTNQDYPRMITTWQ
                     QRERRDGVVLKQLSFPVPPPGLDYLAGRIEEAITPKTRVILICHITNRTGQIFPVKKI
                     CRMARARGIEVIVDGAHAFGHFPFRHADLDCDYYGTSLHKWILAPIGTGFLYVRKSKI
                     PGVWPMMAAPASMNENIRKFEEIGTHPASQRNAITEALNFHDSIGVERKAERLRYLRK
                     RWSNRLQKLPGVKILNSEDPEQSCGIGFVSVEAFDAAKLADHLWQKHRIWTTAIDTPG
                     EYQGLRITPNVYTTLEEIDTFAEVMEKLIRAGKLPA"
BASE COUNT          729 a         1007 c         1030 g          624 t
ORIGIN      
        1 atctgctgga tgcgccagtc gaattttcga atagcgcgga cttgttttgc ctcgaccttt
       61 acaggcactt cgatatcgaa cccctcacac aagccgccac gaagacccgc attgtgtgga
      121 gatgaacggg gatgccagac acccgcgcca taagcctcag cgccattgtg tctgctgaag
      181 ggattcttcg aaagaaatga tggcgaagtg ttggggctgg ctatagcgaa atctcctgtg
      241 cttggcgaga agcaccgggt ggggagcctc ttgccggcag gtgctacgat ctgttttaaa
      301 tgcacgcgca gaagttcctt ccggacatga cgatcactcg ccgtaacctg atgctgccgg
      361 ccgcggctgc ggtgttcgca caaacgggag agccttcgtg gctgcggcag gtgatcgatc
      421 gccacgactc acacgtcgag caccttttga agcagcagat caccagccct cgagaccggt
      481 ggcgaggcat tttcccggat gagtatgggc tttattttcc ggccgcggcc gcaggcatta
      541 tcgacgcgat ggcagccgcc tacctgcatc ccggatcgaa attccacaag aacgccgaac
      601 tgccgcggcg aatccaactt gcggcggatc tgcttgcacg cgagcagacg agcgacggca
      661 actgggactt gccgatcacg aatttcaact ctcctcccga tacaggattc atcctgagaa
      721 acctgtgtcc gccggcggtt cttctgaagc gtgcacagga aacggagctc ttcggccagc
      781 tcgagccgct cttgcgcaaa gcgggaggcg gaatcagcaa gggcggcgtt cacacgccca
      841 atcaccggtg ggttatctgc gcggcgctgt cgcaactcta tgagctattc gaggagccgg
      901 gctatctgaa gcggatagac cagtggctgg ccgagggcat cgacatcgac ccggacggac
      961 aattctccga gcgcagcacc tacgtctata atccggtaac ggataacgcg ctcacgacaa
     1021 tggcggcgaa actaaatcgt ccgcacctgc tcgagcccgt acggaacaac ctcgagagca
     1081 tgttgtatct gctgcatccg gagcatgaag tagtcaccga aatctcgcgg cggcaggacc
     1141 ggaaccagcg cggcgacatg ggcccgtact ggttttccct cgcgtacctg gcgcggcacc
     1201 acgataacag agatggccgg ttcgaaacgc tcgcggcaca tttcgctccc acgcgagcaa
     1261 gcctgagcgc gctgatggaa tatccggaac tggcggcacc cggacccgcg ctcgtttccg
     1321 tgccggacca ttatcgcagg gcgtttccgc ataaccagct cttccacatc cggcgaggcc
     1381 gtatgagtgc cgtcgtgctg gcgggcggac gcagccggtt ctttaccctc cgcaacggca
     1441 acgcggtggt gaacgcggta cgcttcgcaa gcgcgttctt cggtaagggg cagttcagtg
     1501 cgccagtcat cgaggaagcg ggcaacacat tccgcatgac acaacggctc gaggcgcctt
     1561 attatcagcc gctcgatccg ccagagcgcg taaatgccgg ccagtgggag gcggtacgcc
     1621 ccaaacgaaa gaagagcgag atctgccgct tgacgcaatc ggttacaatt tccgaacacg
     1681 ggacgggttt caggctgcgg attcggtccg aggggacaaa ggacgttccg ctggccgtcg
     1741 agatcaatct ccgcgagggc ggtaaactcg aaagcgtcgt accggcgcag aaagtgaccg
     1801 agggatggat tttcgaatca ggccacgcga tctatcgcgc aggcccggat gcaatccggt
     1861 tcggccccgg cactcgcgcg catgtttata cgcaggtccg gggagccgag ccgaagctgc
     1921 ctgggccaag cgtctatttg acgggcacga cgccgttcga tcacaccatc gccttcgagt
     1981 gcctatagga gtaatccatg aaccgccgaa cgttcttccg gtccgccgct ggagccgggg
     2041 ccttatttca agtggacgcc gtccggaggg cacaggcggc tgtccaatcc atatcgggac
     2101 ggcccgcaga cctggtcgca cgcgacgagg acttttggct caacatccgg cacgcgttca
     2161 cggtcgatcg caacctgatc aacctgaata acggcggggt atcgccatcg ccgcgcatcg
     2221 tgatggatac cgaaaagcgg tatctggaga tcgagaatat gacgccgtcg tactacatgt
     2281 ggcacgtcct cgatcccggg atcgaaacgg tgcggcggcg gctggctcgc gagttcggat
     2341 gcgactccga agagattgcc atcaccagga atgcgagcga agcgctcgag atcgtacaac
     2401 tcgggctcga cctgaaaccg ggcgacgaga tcctgacgac gaaccaggat tatccgcgga
     2461 tgatcacgac gtggcagcag cgtgagcgcc gcgacggagt agtgctgaag cagttgagct
     2521 tccccgttcc gccgccgggt ctggattacc tcgccggacg tatcgaagag gcgattacgc
     2581 cgaagacgcg ggtcattctg atctgccaca tcaccaaccg cacgggacag atttttcccg
     2641 tgaagaagat ctgccggatg gcgcgcgcac gcgggatcga ggtgatcgtc gacggcgccc
     2701 atgcgttcgg ccattttccc ttccggcacg cggacctgga ctgcgactat tacggcactt
     2761 cccttcataa atggatcctc gcaccgattg gtacgggctt tttgtatgtc cgcaaatcga
     2821 aaatccctgg cgtctggccg atgatggcgg cgccggcgtc gatgaacgag aatatccgca
     2881 agttcgagga gatcggcacg catccggcgt cacagcgcaa tgcgatcacg gaagcgctca
     2941 atttccacga cagcatcggc gtggagcgaa aagccgaacg gcttcgatac ctgaggaaac
     3001 gctggtcgaa ccggctccag aagctgccgg gggtgaagat cctgaacagc gaggacccgg
     3061 agcagtcctg tggcatcggg tttgtcagcg tggaggcctt cgacgcggcg aaactggccg
     3121 accacctgtg gcagaagcat cgcatctgga ccacggcgat agatacgccg ggcgaatacc
     3181 aaggtctgcg tatcacgccg aacgtttata caactcttga agagatcgac acgtttgccg
     3241 aagtgatgga gaagctgatc cgcgcaggca aactgccggc gtaactcgtc aaggcatccg
     3301 gcgagacaaa acgagccggc actgtaacgc cccctgcgct accggcgtct gatttgatgt
     3361 gaaaaccctt gcgctgcttt taattacggc
//
LOCUS       DASTMK010000437        11734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_54870, whole genome shotgun sequence.
ACCESSION   DASTMK010000437 DASTMK010000000
VERSION     DASTMK010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54870"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VFQ79_18705"
     CDS             <1..1055
                     /locus_tag="VFQ79_18705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141760.1"
                     /translation="TFLSVLAIGVEVTMMLTLVGLSHGMLDESAQRARGVGADIVVRP
                     PGSSVISLSSAPMSDKFIQFFEKQPHVSLAVGTMVHPIGGINTITGIDLDRFNRISGG
                     FRYQSGGPFEGPDSIIVDEFYATQNNLKAGSMHDLLNRQWRVAGVVEPGKLARIFVQL
                     PVLQELTGNTGKLSQIYLKVDDSALDDEVVRQLRSIPALKDYYIYTLEEFTSLISVTN
                     VPGLRAFIYVIIGLSVVVGFIVVFMAMYTAVLERTREIGVLKALGASSGYILNLLVRE
                     TVLLALLGSVLGILMTFGSRWLIETLVPASLTQQIVPSWWPIAAGIAIVGALLGALYP
                     GWKAARQDPIEALAYE"
     gene            1048..1743
                     /locus_tag="VFQ79_18710"
     CDS             1048..1743
                     /locus_tag="VFQ79_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0141761.1"
                     /translation="MNSPCIIEVRDLRKTYRAGEVDVHALRGVNLDVSQGEFISIVGP
                     SGSGKSTLFNVVGGLTPPTSGTVKIGGKDLAGLSDADRTNLRKTMVGFVFQKYNLLPT
                     LSARDNIEIARHIAGRNSNTDARFQQVLELLGIGHRLHHKPRAMSGGEQQRVAIARAI
                     VNDPAILLADEPTGNLDTENSAIVLDLLRDLNERMGQTILMITHNPEAAAYGHRTVHM
                     RDGSIVDAQLTLE"
     gene            1762..2817
                     /locus_tag="VFQ79_18715"
     CDS             1762..2817
                     /locus_tag="VFQ79_18715"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0141762.1"
                     /translation="MYSGFATPEGTARFASRFPGQTAAGFFRQAQGLTVSSLGIGTYL
                     GNLDDATDSSYAASVSRAVLGGINFIDTSLNYRNQRSEISVGAALHGLFDSGAAKRDE
                     IVVATKAGYLVPNAVPAGLRKADLVGGMHCMTPEFLVDQIERSRRNLGLETIDIFYLH
                     NPETQLAEVQANEFYRRIREAFIALEEAAGRGMLRFYGTATWDGYRRPVGDRARISLT
                     RMVQLAEDAGGPGHRFRFIQLPFNLAMPEAYLDKSEEGRSVMEAARAAGIEVVGSASI
                     LQARLARGLPEQLVERMPGTRSDAQRALQFSRSTPGIIVSLVGMSTPAHVEENLEIAT
                     IRPLTEASYLDLFRPQS"
     gene            2819..3946
                     /locus_tag="VFQ79_18720"
     CDS             2819..3946
                     /locus_tag="VFQ79_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141763.1"
                     /translation="MDEAPPEIIELAGSPEEIERRIEAVPDAPAVFAVWAREGAPYLG
                     RTNVLRRRLRRLLTPRSGTSRMLNLRALAVRVEFWLTGSRLESAVLNYELARRHFPDL
                     YRKIVKLPSPAYVKVLLANRFPRCQVTSKTSSGSSMYFGPFRTRASAEQFQEQFLNLF
                     QLRRCTEDLDPSPSHPGCIYGEMGKCLRPCQQVVGEEEYRSEVERVTVFLASGGRSML
                     ERIAGIRDRLSEQLDFEQAAYQHSRYEKVQAVLALRDDLASDVRRIHGVCITPSVADN
                     AVELWFVVKGCFHSRSRFEVGASSSGMVPLDARLRQVLAGLAPSKCLPRHRDENLALL
                     SRWYHASDCDGEWLPFEDLSQVPYRKLVNAIHRVSARGRSH"
     gene            3982..5502
                     /locus_tag="VFQ79_18725"
     CDS             3982..5502
                     /locus_tag="VFQ79_18725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0141764.1"
                     /translation="MERTCVSAALLLLLAAQGLQSASQESESAVLRVTTHVVEVSVVA
                     TPKNGQMDRELTAADLRLWDNGKEQVIRSFEKLSSVTTPADDLEPGVFSNRFSARPQI
                     FSIILLDALNTSWANTASARAEVLRAITQIRPGERIAIFVLGDGLRVVHDFSSDSASL
                     LRKLNSAAGIGPLGRASGTPRRLSAAFSSEMRHRRMEGGAARNFFQTGRILDTFAALE
                     AVANHVKGLPGRKNLLWVSAAFPLGVGNMGQLRTFGAEMERAARALNSADIAVYPIDA
                     RGLFVGNPYVNVSTMKALAEETGGTAFYNRNDLHQAVRAALDDTRDVYSLSYSPQDFS
                     EDGSFHRIRLETSRRDIQLRYRRGYYASQATNEEKLPAAATLMDSAFGSPLDASEIGL
                     TVSVEREAADGKDLSVLIRIDPHDLNLRRQGDRWTGAISVGLVQLGAAGERRGEAVKE
                     MGISISPEDYDRVRRGNFAFRMRIRQEKGASSLRVAVVDKASAHVGSLSVPFPDTL"
     gene            5580..5918
                     /locus_tag="VFQ79_18730"
     CDS             5580..5918
                     /locus_tag="VFQ79_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141765.1"
                     /translation="MKRSLLASLTVASVLLLASCGSVKIGRILDNPTRYQDRSVSIEG
                     TVTNSFGAFVAGGYQVQDETGQILVLASGSVPRKGVKVKVDGRVQSGVTFMGRSYGTT
                     IRERDHKVKY"
     gene            complement(6003..6368)
                     /locus_tag="VFQ79_18735"
     CDS             complement(6003..6368)
                     /locus_tag="VFQ79_18735"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0141766.1"
                     /translation="MVQYNSLYWDEHLEVDFQRNLVTLDSVPLVLTRKEFELLALLVA
                     NACEIVPRDVLLMRVWGYSREIRTRTLDVHIRRLRKKLNRYGDQYIETIFGVGYRFQR
                     YREPRLIPSYLPALMATVA"
     gene            complement(6497..6865)
                     /locus_tag="VFQ79_18740"
     CDS             complement(6497..6865)
                     /locus_tag="VFQ79_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141767.1"
                     /translation="MLATLLYAADGKRKPPKPPDLEVIELTARRTEGRILVDFRIRNS
                     GLKTLENVVVLLDFLAPENKVVTTQRAEIEPAILDPGEEAEFHGQLRDPARAVRCRIN
                     SQDGAGRELTVSGSGPFVID"
     gene            6947..8017
                     /locus_tag="VFQ79_18745"
     CDS             6947..8017
                     /locus_tag="VFQ79_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEU0141768.1"
                     /translation="MFSRREWLLGSAAFSGAAGYQAGGRDIVIASSNGLGACQRAMAV
                     LRSGGDTLEAVVEGVTLVELDPGDMSVGYGGLPNEEGVVELDACVMHGPTRRAGSVAS
                     LRNIKTPARVAKLVMEQTDHIMLAGEGALRFAKAYGFQEENLLTEKARLAWLVWKQSL
                     RDNKGHNNWGPGLDAPDGKPSASLRNAFPGVDEATWAWAIEVARRPPTGTINCLALNS
                     KGEMSGVTTTSGLAWKIPGRVGDSPIIGAGLYVDQDVGAAGSTGRGEENIRVAGAHTI
                     VENMRHGMEPQEACIDALKRVARNYNGNREKLAKFDLSFYALRKDGQYGAASLWSGRK
                     FAVNTGTGSRHENAAHLFERKD"
     gene            complement(8018..9259)
                     /locus_tag="VFQ79_18750"
     CDS             complement(8018..9259)
                     /locus_tag="VFQ79_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU0141769.1"
                     /translation="MLRRFRYHIAVFFSVIGPGFITAVVDNDAGGIYTYSQAGAKYGY
                     LPLWTLIPITVLLIVTQEMCSRMGAVTGKGLSDLIREEFGLRTTFLVMSALVLANVTN
                     VMANFAGVASSLELFQVSKYVSVPLGAMAVWLLVVKGNYQSVEKIFLFATALYVCYIV
                     SGVLVKPDWEEAMISSVRPEIAFETGYIQMLIAMVGTSVAPWMQFYLQAAVVEKGITA
                     KEYAESRIEVIVGCIVMTVIAFFIIVACAGAIWANGPRDIETAADAALALRPFGQYAY
                     LLFCAGLLNASLFAASILPLSTAYTVCEGLGFESGVNKRFREAPVFYWFYTLLIVIGA
                     GVILIPGFPLIDMILLSQVINGVLLPFVLIFMILLVNRRGLMREWTNSKSYNAVAWAA
                     VAVMIGLTLALVGISIRDLGR"
     gene            complement(9273..10580)
                     /locus_tag="VFQ79_18755"
     CDS             complement(9273..10580)
                     /locus_tag="VFQ79_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0141770.1"
                     /translation="MAAWPELGVGALSVSYWSAAMATADHILYLTELIGLPVFDLKGR
                     RIGRIRDAALVPLINPMRVDRYLIGGGWAWLTIRHDQVQSISIDGIHLRDEQLTPYHS
                     DEYMLRMNRDLLDQQIIDVHGRKVVRVTDITFEIRRVDGHDELRIVEVDIGIRSILRR
                     LLQGVAPRKLIRRFQSSIPPNSIRWEFCNIVESDPQRRLRLNISNQMLEKMHPADLAD
                     IVEELSPEDRGAIFSTIDSEVAAEALSEVDPEIQASILESLEAEKAADIVEEMSPDQA
                     ADVLAELEEETSDEILEEMEMEPKSEVQELLEFGEDTAGGLMNTEYVSMEPGATVDGA
                     IEAMKIQEELLEVLNTIFLVDADGRLTGAVPLAKLFVVPGSTTLQSLAVETLIHVPVN
                     EKSDRVTELFDKYNLLTLPVVDEEGRMAGVITADDIITVLRSR"
     gene            10712..>11734
                     /locus_tag="VFQ79_18760"
     CDS             10712..>11734
                     /locus_tag="VFQ79_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0141771.1"
                     /translation="MSLSMPSPLVRGLFAIIIIALVVLVHLKLLRWVTESHTRRRSIT
                     WLLRGLAALSAFWILASVFVRRAPGLDYVPVWLAWMFGLGITWGFCSLLVLVLSVIWR
                     RLPAFNPRRRGFLVASRTALIAAPAAVAGFGVFIGRKDIGVREIDLRMPALPKDLDGF
                     RLVQLSDIHLGPFFSQDDLARAVDMANDTRAHLAVVTGDLITRMGDPLDGCLQELSRL
                     KADFGVWGCLGNHETYANTEDYTEREGARRGIRFLRQRAHRLSKGSARLNLAGVDYQS
                     VRRPYLENAESLVAPDSFNILLSHNPDVLPIAARKGFDLTIAGHTHGGQINVEILAQN
                     ISLARFI"
BASE COUNT         2354 a         3341 c         3563 g         2476 t
ORIGIN      
        1 gcacgttttt gagcgtactg gccattggtg tcgaagtgac gatgatgctg acgctggtcg
       61 gcctgagtca cggaatgctc gacgaatcgg cacagcgagc ccgcggtgtg ggcgcagata
      121 ttgttgtacg cccgcctggc tcctctgtca tcagtctcag ctcggctccg atgtcagata
      181 agttcatcca gttctttgag aagcagcctc acgtctcgtt ggcagtggga acgatggtac
      241 accccatcgg cggcattaat acgatcacgg gaattgacct cgacaggttc aatcgcatca
      301 gcggcggctt ccgttatcag agcggcggtc ctttcgaggg cccggacagc attattgtcg
      361 acgaatttta cgccactcag aacaacctga aagccgggag tatgcatgac ctgctaaacc
      421 ggcagtggcg agtcgccggt gtcgtagagc cggggaaatt ggcccgcatt ttcgttcagt
      481 tgcctgttct tcaggaactc accggcaata cgggaaagct gagccagatc tatctgaaag
      541 tggacgactc cgcgctggac gacgaggtgg tccggcagct acgctccatt cccgcgttga
      601 aggactacta catctacacg ctcgaagagt tcacatcgct gatttcggtc accaatgtgc
      661 cgggcctgcg ggccttcatc tacgtgatta tcggtctctc cgtggtcgtc gggttcattg
      721 tcgttttcat ggcgatgtac acggcggtcc tcgaaagaac gcgcgaaatc ggcgtgctga
      781 aggcgctggg cgcttcctcc ggatatatct tgaacctgct ggtacgcgag accgtgctgc
      841 tggccctgct tggatcggtt ctcggtattc tgatgacctt cggcagccgc tggctgattg
      901 agacgctggt gccggcttcc ctcacccaac aaattgtgcc gtcctggtgg cccatcgcgg
      961 caggcatcgc tattgtggga gcgttgctgg gagcgctgta tcccgggtgg aaagctgccc
     1021 gacaggatcc gatcgaggcc ctggcgtatg aatagtccgt gcatcatcga agttcgggat
     1081 ttgcgcaaaa cctaccgcgc gggagaggtg gatgtgcatg cgctgcgtgg ggtgaacctc
     1141 gacgtcagcc aaggggagtt catcagcatc gtaggtccat cgggttccgg caagtccact
     1201 ctgtttaacg ttgtgggcgg actcaccccg ccgacttccg gaaccgtcaa gatcggcggc
     1261 aaagacctgg cggggttgag cgatgcggac cggacgaacc tgcgaaagac gatggtcggg
     1321 tttgttttcc agaagtacaa cctgttacca acgctctccg cgcgagacaa catcgaaatt
     1381 gcacgccaca tcgcaggccg gaattccaat acggatgccc gttttcagca ggtgcttgag
     1441 cttctgggaa tcgggcatcg cctgcaccat aaacccaggg caatgtccgg cggcgagcag
     1501 cagagagtcg ccattgcccg ggccattgtg aacgaccccg cgattttgct tgccgacgag
     1561 ccgaccggca acctggatac ggaaaactct gcgatcgtgc tcgatctgtt gcgcgatttg
     1621 aacgaacgaa tgggacagac cattctcatg attacccaca atccggaagc cgcggcctat
     1681 ggccaccgga ccgttcatat gcgcgacggc tctattgtag acgcgcaact gacgctcgaa
     1741 tagcgcggtt agaatcaaga aatgtattcg ggttttgcga ctccggaggg caccgcgcgg
     1801 tttgcctcgc gttttcccgg ccaaaccgct gccggcttct tccggcaggc gcaaggattg
     1861 acggtctctt ccctgggcat tgggacttac ctgggcaatc tggatgatgc tacagactcg
     1921 tcttatgcgg catcggtttc ccgcgccgtc ctcggtggca tcaatttcat cgacacctcg
     1981 ctgaactacc ggaaccagag gtccgagatc tccgtcgggg cggcacttca cggtcttttc
     2041 gacagtggag cggcgaaacg cgacgaaatc gtagttgcga ccaaagcggg ctacctggtt
     2101 ccgaatgccg tgccagcagg actgcggaag gctgatctcg tgggtggcat gcattgcatg
     2161 actccggaat ttctggtgga tcagatcgaa cggagccgcc gcaacctggg tctggagacc
     2221 atcgacattt tctatctcca caacccggaa acgcagttgg cggaggtcca ggcgaacgaa
     2281 ttctaccgtc gcatccgcga agcgttcatc gcactggagg aggcggccgg gcgcggaatg
     2341 ttgcggttct acggcaccgc gacatgggac ggctaccggc ggccggtggg tgatcgtgcg
     2401 cgtatctccc taacccgcat ggtgcaatta gccgaggacg cgggaggccc gggccatcga
     2461 ttccggttca tacagcttcc cttcaacctg gccatgccgg aggcgtatct cgacaagtcc
     2521 gaagaaggcc gctccgtgat ggaagccgcg cgagcagcgg gaatcgaagt cgttggcagc
     2581 gcttccattc tccaggcgcg ccttgcccgc ggactgcccg aacaactcgt ggaacggatg
     2641 ccgggtactc gaagcgatgc gcagcgcgcg cttcagttca gccgctccac gccgggaatt
     2701 attgtttcgc tggtagggat gagcacgccc gcgcacgtgg aggaaaacct ggagatcgcc
     2761 acgatccggc cgctgacgga agcaagttat ctcgatctgt tccgtcctca atcgtgacat
     2821 ggatgaggcg cctcccgaga tcatcgaact cgccggttca ccggaggaga tcgagcgccg
     2881 catagaggct gtgccggacg ccccggcggt ttttgccgtc tgggcgcgtg aaggtgcgcc
     2941 ttatctcgga cgcacgaacg tactgcgccg ccgtcttcgg cgcctgctca ccccgcgttc
     3001 cggaacttcc agaatgctga acctgcgggc tcttgccgta cgggtcgagt tctggctcac
     3061 tggatcgcgc ctggaatcgg ctgttctgaa ttatgagctg gcgcgccggc actttccgga
     3121 tctgtaccgg aaaatcgtaa agctgccgtc tcctgcttat gtgaaagtac tgctggcgaa
     3181 ccgtttcccg cgctgccagg taacatcgaa gaccagctcc ggttcctcaa tgtactttgg
     3241 gccttttcgt acccgcgcat cggcggagca gtttcaggag cagtttctca acctgttcca
     3301 gttgcggcgc tgcacggagg atctggaccc ctcgcccagc catcccggct gtatttacgg
     3361 agaaatgggt aaatgcctgc gcccgtgcca gcaggtggtg ggggaggaag agtaccggtc
     3421 ggaagtggag cgcgtcacag tgttcctcgc aagcggaggc cgctcgatgc tggagcgcat
     3481 cgccggaatc agggatcgtc tgagcgagca gttggatttc gagcaggcgg cctaccagca
     3541 cagccggtac gagaaagtac aggccgtcct tgcgctgcgg gacgatcttg cctccgacgt
     3601 gcgacgcatt catggagtgt gtatcactcc ctcggtggcg gacaacgccg ttgagttgtg
     3661 gttcgttgtc aaaggttgct ttcactcccg cagccgtttc gaagtgggtg cttccagcag
     3721 cggcatggtg ccgctggatg cgcgtttgcg gcaggttctg gccgggcttg cgccttctaa
     3781 atgtctgccg cggcatcgcg atgagaacct ggcattactt tctcggtggt atcatgcaag
     3841 cgactgcgat ggcgaatggc ttcccttcga ggatttgagc caggttccgt accggaagct
     3901 ggtgaatgcg atccaccggg tgagtgcccg gggacgctct cactgaatcc gtatggagcc
     3961 tcgttttcgc ggcttcaaaa tatggaaaga acatgtgttt cggcggcttt gctcctgctc
     4021 ctggctgccc agggattaca gtcggcgtcg caggaatctg agtcggctgt attgcgtgtg
     4081 acgacccacg tcgttgaagt tagcgtggtc gcgactccca agaacggtca gatggatcgt
     4141 gaactaacgg ccgcggatct tcgcttgtgg gataacggca aggaacaggt tattcgatct
     4201 ttcgaaaagc tctcatccgt taccaccccc gcggacgatc tcgaacctgg tgtgttttcc
     4261 aatcgcttta gcgcacgccc gcagattttc tccatcattc ttttggatgc tctgaataca
     4321 tcatgggcaa acacggccag tgcacgagcc gaggtcctcc gtgcgattac tcagatccgt
     4381 ccgggggagc gcatagcgat tttcgtgctc ggcgatggtc ttcgcgttgt gcacgacttt
     4441 tcttccgaca gcgcttcgct actccgtaaa ctcaactcgg ccgccggcat cgggccatta
     4501 gggcgggcgt ccgggacgcc gaggcgcttg tctgccgcgt tttcctcgga gatgcggcac
     4561 cggcgtatgg aaggcggggc ggcgagaaac ttctttcaga ccggtagaat tctggacaca
     4621 tttgccgcac tggaagccgt cgccaatcac gtcaaagggc ttccgggcag aaagaatttg
     4681 ctttgggtct cggccgcctt ccccctgggc gttggcaata tggggcaact gcgcaccttc
     4741 ggcgccgaga tggagcgagc agcgcgggcg ctcaactcgg cggacatcgc ggtatatccg
     4801 attgatgcca ggggactctt tgtcggtaac ccgtatgtta acgtctccac gatgaaggca
     4861 ttggctgaag agaccggcgg aacggcgttc tacaaccgca acgacctgca tcaggctgtg
     4921 cgcgccgcgc tggacgatac acgcgatgtt tactcgctca gctactcgcc tcaggatttc
     4981 agcgaagacg ggtcgtttca ccgcatccgg cttgagacat cgcgccgcga tattcagcta
     5041 cgataccggc gagggtatta tgcttcccaa gccacaaacg aggaaaagct gccggcggcc
     5101 gcaaccctca tggattcggc attcgggtcc ccattggacg catcggagat cgggctgacc
     5161 gtcagcgtgg agcgggaagc tgcggacggg aaagatttgt ctgttctgat ccggatcgat
     5221 ccgcacgacc tgaatttgcg gaggcaaggg gaccgctgga ccggcgcgat cagcgttgga
     5281 ttagtccaac tgggtgcggc cggagagcgg cgcggagagg cagtgaaaga gatgggcatc
     5341 tccatttcgc cagaggatta tgaccgcgtg aggaggggaa atttcgcgtt ccggatgcga
     5401 atccggcagg agaagggagc gtcatctctt cgcgtggcgg ttgtagacaa agcgagtgcg
     5461 cacgtgggtt ccctttcagt accttttcca gatacacttt gataccggtc tggtttagtc
     5521 tcattgaata tacgcaaccg cacagattgg gttgtgcgtc tatgaggcgg ggaggaagaa
     5581 tgaaacgatc gttgctcgcg agtcttaccg tggcttccgt tctgctgctt gcttcgtgcg
     5641 ggtcggtcaa gatcggcaga atattggata atcccacgcg ataccaggac agatcggtca
     5701 gtatcgaggg taccgttacc aattcatttg gtgcgttcgt cgccggaggt taccaggtgc
     5761 aggacgagac cggacagatt ctcgtgctgg ccagcggctc ggttccacgc aagggagtca
     5821 aggtgaaggt cgacgggcgg gtgcagagcg gcgttacgtt tatgggccgg tcctacggaa
     5881 ccacaattcg cgagcgcgat cacaaagtaa aatactgaaa atccagccgg attgtctccc
     5941 cgtttggagc ggcggctcgg tcacgatccc cgcggccgag ccgcacgcaa gcaggtgcgg
     6001 tgtcaggcga ctgtagccat gagggcgggc agataggacg ggatcaggcg cggctcccgg
     6061 taccgttgaa acctgtagcc tacaccgaaa atcgtctcga tgtattggtc tccatatcga
     6121 ttgagtttct tgcgcaacct gcggatgtgc acgtccagag tgcgtgtacg gatctcgcgg
     6181 ctataacccc agactctcat caaaagtacg tccctgggaa cgatttcgca cgcgttggcg
     6241 accaaaagcg cgagcagttc gaattctttc ctggtgagga ccagcggcac ggaatccagc
     6301 gtgaccaggt ttctctggaa gtccacttca aggtgctcat cccagtaaag cgagttgtat
     6361 tgaaccatca ctttgctccc tactgtgatg ctagggtgcg gtccgcggaa ggtcgtggct
     6421 aacttgtaaa agaaatgtaa aaccggtggc ggtgggcttg catattgccc accggctgag
     6481 cttgacgcct ggtacattaa tcgataacaa aggggccgct gccggagacc gtcagctcgc
     6541 gtccggcgcc gtcttgcgag ttgatccggc accgtaccgc ccgtgccggg tcccggagct
     6601 gtccatggaa ttcggcttcc tccccgggat cgaggatcgc cggttcgatt tcggcacgct
     6661 gggttgtgac cactttgttc tccggggcca gaaaatcgag cagaaccacg acattctcga
     6721 gagtcttcag cccgctgttt cgaatgcgaa aatccaccag aatcctgccc tccgtccttc
     6781 gggcggtcaa ctcgataact tcgagatccg gcggcttcgg aggcttgcgc ttcccatcgg
     6841 ccgcatacaa caatgtggca agcatcaggc agcttattac gagacgcccc atgatgaaga
     6901 tagtgtagct gaagcggcgc gtcttggcgt gtgaggagga gattttatgt tcagcaggcg
     6961 ggagtggctg ctcggctcgg cggcattttc cggagcggca ggctatcagg cgggaggccg
     7021 cgacatcgtc attgccagct ccaacggcct cggcgcctgt cagcgggcga tggctgtcct
     7081 gcgatccgga ggcgatactt tagaagctgt cgtggaagga gtgacactgg tcgagctcga
     7141 tcccggcgac atgtctgtcg gttatggcgg gctgcccaac gaagagggcg ttgtggaact
     7201 ggatgcctgc gtgatgcacg gacccacgcg gcgggcgggc tcggtggcct cgctgcgaaa
     7261 catcaagaca ccggcccgtg ttgcgaaact ggtgatggaa cagaccgacc acatcatgct
     7321 tgccggggaa ggcgcgctgc gcttcgcgaa ggcttacggt ttccaggaag agaacctgct
     7381 gacggagaaa gcccgccttg cgtggctggt ctggaagcag tcgctgcgcg acaataaggg
     7441 ccataacaat tgggggccgg ggctggacgc gccggatggt aagccatccg cctcgctgcg
     7501 gaatgctttt ccaggggtcg atgaggcaac gtgggcctgg gccatcgaag tggcgcggcg
     7561 tccccctacc ggcacgatta attgtcttgc cctgaattcc aagggcgaga tgtcgggtgt
     7621 aacgaccacc agcgggttgg cgtggaaaat tccgggtcgg gttggcgatt cgcccattat
     7681 cggcgccggg ctctacgtcg accaggatgt cggagcggcg ggctccacgg gccggggtga
     7741 agagaatatc cgggtcgccg gtgcgcatac cattgtcgaa aacatgcgcc acggaatgga
     7801 gccgcaggaa gcctgcatcg acgctctgaa gcgcgtggcg cgcaactaca acggaaaccg
     7861 cgagaagctt gcaaagtttg acttaagctt ttacgcactg cggaaagacg gccagtacgg
     7921 tgccgcgtcg ttatggagcg ggcggaagtt tgccgtgaac accggtacgg gcagccggca
     7981 cgagaacgcg gcccatctgt tcgagcgaaa ggactgatca gcggcccaga tcccggatcg
     8041 aaatccccac caacgcgagc gtcaggccga tcatcactgc cacggccgcc caggccaccg
     8101 cgttgtacga tttcgagttg gtccattccc gcatcagccc gcggcggttc acgagaagga
     8161 tcatgaaaat caggacgaag ggcagcaaaa cgccattgat cacttgcgag agcaggatca
     8221 tgtcgatgag agggaatccc gggattagaa tcacgccagc gccaatgacg atcagcagcg
     8281 tatagaacca gtagaaaacc ggagcttcgc ggaaacgttt gttgacgccg gactcgaatc
     8341 ccagaccctc acatacggta taggcggtgg aaagcggcag aatcgaggct gcgaaaagag
     8401 aagcattcaa cagccctgcg cagaagagca agtaggcata ctggccgaag ggacgaaggg
     8461 ccagtgccgc atccgcggcg gtctcgatat ccctggggcc gttggcccag atcgcaccgg
     8521 cgcaggccac gatgatgaag aacgcaatca ccgtcatcac gatgcagccg acgataactt
     8581 cgatcctcga ttcggcgtat tccttcgctg tgatcccttt ctccactaca gccgcttgca
     8641 gataaaactg catccacggt gctacggagg tgcccaccat agcgatcagc atctgtatgt
     8701 agcccgtctc gaatgcgatt tccggccgca cgctggaaat catcgcttct tcccaatcgg
     8761 gcttcaccag aactccggaa acgatgtagc agacatagag ggcggtggcg aagaggaaga
     8821 ttttctccac gctttgataa ttgcctttca ccaccagcag ccacaccgcc atggcaccca
     8881 gcggcaccga gacgtacttg gacacctgaa aaagctcgag ggaactggcc acgcccgcga
     8941 aattcgccat gacattcgtc acgtttgcca ggacgagcgc cgacatcacc aggaaggtgg
     9001 tcctgaggcc gaattcttcc cggatgaggt cggacaggcc tttgccggtc accgcgccca
     9061 tgcgggagca catctcctgc gttacgatca acaggaccgt gatcggtatc agggtccaca
     9121 acgggagata cccgtatttg gcgccggcct gcgaataggt gtagatgccg ccggcgtcat
     9181 tgtcgacgac cgcggtgata aaaccgggac caatgacaga gaaaaatact gcgatgtgat
     9241 agcgaaaccg tctcagcatc gggccggctc gctcacctgc tcctcaagac cgtgatgatg
     9301 tcatcggcgg tgatgacgcc ggccatcctg ccttcttcgt caaccaccgg gagcgtcaac
     9361 aggttgtatt tgtcgaacag ctccgtcacg cggtcgctct tctcgttcac cggaacatgg
     9421 ataagggttt cgacagccag gctttggagt gttgtagagc cgggaacgac gaacagcttt
     9481 gccaaaggga cggcgccggt cagcctgccg tccgcgtcga ccaaaaagat ggtgttcagg
     9541 acttccagca gttcctcctg tatcttcatc gcttcaattg cgccgtctac cgtcgcaccg
     9601 ggttccatcg agacatactc ggtattcatc agcccgccag ccgtatcctc tccgaattcg
     9661 agaagctcct gcacctcgct cttcggctcc atctccatct cttcaaggat ctcgtccgag
     9721 gtttcctcct caagctcggc gagtacatcg gcagcctggt cgggagacat ctcttctaca
     9781 atatcggcgg ccttctccgc ctccagcgac tcgagaatac tcgcttgaat ttcgggatcg
     9841 acttcggaaa gcgcttcggc agccacttcg ctgtcgattg tgctgaaaat cgcgccgcgg
     9901 tcctccgggc tcagttcttc tacaatatcg gccaggtccg ccggatgcat tttctcgagc
     9961 atctggtttg agatgttcag ccgcaggcgc cgctgcgggt cggactcgac gatattgcag
    10021 aattcccaac gaatggagtt ggggggaatg gaggactgaa accttcgaat cagtttccgc
    10081 ggcgccactc cttgcagcag ccggcggagg atgctgcgaa ttccgatgtc cacttcgacg
    10141 atgcgcaatt cgtcatggcc atccacacgg cgaatctcga aggtaatgtc ggtaacccgg
    10201 accactttac gcccgtgcac atcgataatc tgctggtcca gcaagtcgcg gttcattcgc
    10261 agcatgtatt catcggagtg gtacggagta agctgctcgt cgcgcaggtg gatgccatcg
    10321 attgagatgg attgaacctg gtcgtgacgg atggtcagcc acgcccagcc gccaccgata
    10381 agatagcggt ctactcgcat cgggttgatc aggggaacga gagccgcgtc gcgaatgcgg
    10441 ccgattctcc ggcccttaag gtcaaataca ggcaatccga tcagttccgt aaggtacagg
    10501 atgtgatccg ccgttgccat cgccgcgctc caataggaaa ccgagagcgc gccaacccca
    10561 agctccggcc aagcggccat tccggtgcgc tctacattgc tgattcgctc attctcacta
    10621 tgccccaagg cccggaggag ccgcaacaat attcaaattc gctggcaggt ccggaccgcg
    10681 tttcagcatt gcgccggggg agtccgttag aatgagctta tcgatgccct ctccgctggt
    10741 acgcgggtta tttgccatta tcattatcgc gttggtggta ctggtccatc tgaagctgct
    10801 tcgatgggta acggaatcgc atacacgccg ccgttccatc acatggcttc tccggggcct
    10861 agccgcgctt tccgcgtttt ggattttagc aagcgtgttc gttcgcaggg caccagggct
    10921 cgactacgtt ccggtgtggc tcgcctggat gttcgggtta ggaatcacct ggggtttttg
    10981 cagcttgctg gttctggttc ttagcgtgat ctggcggcga ttgcctgcgt tcaatccgcg
    11041 gcgcagaggt tttctcgtgg cctcccgcac tgccctgatt gccgcacccg cggcggttgc
    11101 cgggtttggc gttttcatcg ggcgcaagga cattggcgtt cgcgagatcg atctgcggat
    11161 gccggcctta ccaaaagatc tggatggctt ccggctcgtt caactgagcg atattcacct
    11221 gggcccattc ttttcacagg acgatctggc ccgcgcagtg gacatggcga atgatacgcg
    11281 ggcacacctg gcggttgtaa cgggcgattt gatcaccagg atgggggacc cactcgacgg
    11341 ctgcctccag gaattgtcgc gcctgaaagc ggatttcggt gtctggggct gtctgggaaa
    11401 ccacgagacc tatgcgaata ccgaggatta taccgaacgg gaaggcgcgc gccggggtat
    11461 ccggttcctc cggcagagag cgcaccggct ttcgaaagga tcggcgcggc tgaatcttgc
    11521 cggtgtcgat tatcagtccg ttcgcagacc gtatcttgaa aatgccgaat cgttagtggc
    11581 tcccgattct ttcaacatcc tgttgtccca caatccggac gtgcttccca tagccgcgcg
    11641 caaggggttt gaccttacca ttgccggaca cacgcacggc ggccagatta acgtagaaat
    11701 attggctcaa aatatcagtc tcgcgcgctt catc
//
LOCUS       DASTMK010000438         5566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_182057, whole genome shotgun sequence.
ACCESSION   DASTMK010000438 DASTMK010000000
VERSION     DASTMK010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182057"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VFQ79_18765"
     CDS             complement(<1..1350)
                     /locus_tag="VFQ79_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0141772.1"
                     /translation="MKRVRVLVVDDEPGIRQSLKGVLEDEEFLVDTVGSGEACLDILR
                     REPYDIVLLDVWLPGIDGMEVLSRIQEIPFPERPGVVMISGHGTIETAVRATKLGAFD
                     FIEKPLTAEKVCVIVKNAFQQRRLEIELQRIRQDRDPHIKIIGESVPMKALRQQLSLM
                     AATNGRVLIFGESGTGKELIAHAIHSMSQRATEAFIEVNCAAIPEELIESELFGHRKG
                     SFTGAVDDKIGKFQKADGGTIFLDEVGDMSLKTQAKVLRALEEQRFEPVGASNSVQVD
                     VRVVAATNKNLEEQIERGSFREDLFYRLNVIPFYVPPLRERKEDIPALADHFLREFTT
                     AYGRKQKELTPEALRVLVDYHWPGNVRELRNLIERIVIMNPQVRVDARHIPIHPTRRA
                     VYERPPDRLGTLQEVREAAERDYILRKLEEMKGNVTRTAELLGLERSNLYRKMRSLGI
                     "
     gene            complement(1347..3455)
                     /locus_tag="VFQ79_18770"
     CDS             complement(1347..3455)
                     /locus_tag="VFQ79_18770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0141773.1"
                     /translation="MKKRLINSAGVAVMIALVTLVVWQGSFDFGNYGPSSPQQTYLFW
                     AVSTLVFLLTVTLGFMLFRTAFKLYMERQENREGSRIRTKLIAGALTLSFVPVIFLVI
                     FDIHVLNRNLDKWFTRPAENIKINLAEIGSALEQEIRARIAAQAGQIASMPEIQAYAE
                     TGIRPQNVFESICKQKALDEVLLEAASGASLPVCRINPDELAQRAPLVEARAPVQAAG
                     IFVANLLVRDRIPVDIAREQNEIRREIADYNQLHANKKEFRYFYRALLVLIVLFLLFF
                     ATWLAGVFARRLSLPITGLLEAAREIRSGNLSHRVHVNAIDELATLVRTFNEMTQALE
                     TNSRELERRRRFTETILDSIPTGVLSMSGDGRIQSYNPALKRILPEERVARATTLDDL
                     FSRDDAAEVRYLMKRARRLGVASRQLDYKTGQQTLQLSLTVSALEERATAGFVMVVED
                     TSELLRAQKAMAWHEVARRVAHEIKNPLTPIALSAGRVARQLAKYPVAPEFERIVQEC
                     TATISREVETVKTLVNEFSQFARFPAARPEIADLNAVVQGGLSAFEDRLDGIRIHTVL
                     TPDLPPVNIDREQFKRVVVNLVDNAAEAMQDSLVKELLITTQAVTTDSIELVVADTGV
                     GISPEDKEKMFLPYFSTKGRGTGLGLAIVSHIISEHGGHIRVEDNSPCGARFIIEIPT
                     AGDAERRLVETPAAGSWEQP"
     gene            complement(3564..>5566)
                     /locus_tag="VFQ79_18775"
     CDS             complement(3564..>5566)
                     /locus_tag="VFQ79_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141774.1"
                     /translation="PQYLTGSLFAYDGISPNVSTRSLQAAQDSASRGYNQPEQSYTGN
                     LDFTLGNTSLLSVKGGRYYLNYKEIGVPFLKSYWWQASSVGLPGVPADLQQPFNYATA
                     SAAQVVHDITTRSYVQADFSQFLRFLGSHNLKAGVGTQKNVNNVNDAWVGPDGRVQLY
                     WDLSVASGDRGTYGYYIVDDAATRGSTGASIIHMYIQDSWRVHPRLTLNVGLRTEKET
                     IPSFRRDVQEYAFQFGFGDKIAPRLGASFDVFGDGKMKISGAWGRFYDWTKFDLARGT
                     FGGDLWQQHYRSLDTLDIYSISLNNMPGRNLWSGPYRDRRLPGFEYLDPDMKPMSADV
                     MNIGIEYEVRPQMVFAGRYVRNHLNRTIEDMGALDASGNEVYRYGNPGEGTNTVFPAS
                     GATCTVQVGEACGFEMPKAKREYDAMELSLTKRFGNGWLGNVSYVYSRLWGNYTGLQS
                     TDEIRPPTLGYGFGPNQVFAAENYRPGGNANRYFDLDEAMWDANGRVGLFGRLPTDRP
                     HVFKFYGAKHFSFGTEIGGFFRMMSGTPVTTQVVTVNDIPFYVEGRGDWGRTPFFNQT
                     DLMVAHEVRLGEVKRLRFEFNMDNLFNQKTSMYVFDRYNREEHSDSAGINLSNTDLSR
                     GFDWRSMVAATPDGARALDQRYGKDGIFNPGFQGRFLVKFIF"
BASE COUNT         1121 a         1634 c         1606 g         1205 t
ORIGIN      
        1 gattccgagc gaacgcattt tccggtataa gttgctgcgt tcgagcccga gcagttcagc
       61 ggtgcgagtg acgttcccct tcatctcctc gagcttccgt aggatgtagt cccgttcggc
      121 ggcttcacgg acctcctgta gcgtgccgag gcggtccggc ggacgttcat aaacagcacg
      181 gcgggtcggg tggatcggga tgtgcctggc atcgacacgg acctgcgggt tcatgatgac
      241 gatgcgttcg atgaggtttc tcagctctcg cacattaccg ggccagtgat aatccaccaa
      301 tacgcgcaaa gcttccggtg tcagctcttt ctgcttgcgg ccgtaggcgg tggtgaactc
      361 gcggaggaag tggtccgcaa gcgcaggaat gtcctctttc cgctcccgca gcgggggcac
      421 atagaaggga atgacgttca accggtaaaa gaggtcctcc cgaaagctcc cacgctcaat
      481 ctgctcttcc aggtttttgt tggttgccgc caccacgcgc acatccacct gcacggagtt
      541 cgaggcgccc acaggctcaa agcgctgttc ttcgagagca cgcagaacct tcgcctgcgt
      601 cttcagactc atgtcgccta cttcgtcgag gaagatggtt cctccgtccg ctttctggaa
      661 tttcccgatc ttgtcgtcga ccgcgcctgt aaagctgccc ttgcggtggc cgaacagctc
      721 gctttctatc aattcttccg gaatcgcggc gcagttcact tcgatgaacg cttctgttgc
      781 gcgctggctc atggagtgaa tggcgtgagc gatcagctcc ttgccggttc cgctttctcc
      841 gaaaataagt acgcggccgt tagtggcggc catcaggctg agctgttgcc gcagcgcttt
      901 catcggtacg ctctcgccga tgatcttgat gtggggatcg cgatcctgcc ggatgcgctg
      961 caattcgatt tcgagccggc gctgttggaa cgcgttcttt acgatgacgc agaccttttc
     1021 cgcggtcagg ggtttttcga tgaaatcgaa cgcgccaagc ttggtcgctc tcaccgcggt
     1081 ttcaatggtt ccgtggcccg agatcatgac cacacccgga cgctccggaa aaggaatctc
     1141 ctgaatgcgg gacagcactt ccataccgtc tatcccgggc aaccagacat cgagtaggac
     1201 aatatcgtag ggttcacgcc gcaaaatgtc gaggcatgct tctccactgc cgaccgtgtc
     1261 taccagaaac tcttcgtctt cgagtacacc cttaagtgac tggcgaatgc ccggttcgtc
     1321 gtcgacgacg agcacgcgga cccgcttcat ggctgttccc aggatccggc ggcgggcgtc
     1381 tccaccagtc ttcgctcagc atcacccgcg gtgggaattt cgatgatgaa cctggcgccg
     1441 caaggactgt tgtcctccac ccggatgtgc ccgccatgct cggaaatgat atgactcacg
     1501 atcgccagac cgagtcctgt tccgcggcct ttggtgctga agtacggcag gaacatcttc
     1561 tccttgtcct cgggagaaat gcccacacca gtatcggcca ccaccagctc gatggaatcg
     1621 gttgttaccg cctgcgtggt gataagcagt tccttcacca ggctatcctg catggcttcc
     1681 gccgcgttat cgaccagatt caccaccacg cgtttaaatt gctcgcggtc gatgttcacg
     1741 ggaggaagat ccggagtaag cacggtgtgg atacgaatgc cgtccaggcg gtcttcaaat
     1801 gcggacaggc ctccctggac caccgcgttc aaatcggcaa tctccggccg cgcggcgggg
     1861 aaacgcgcaa attgagagaa ctcgttcacc agggtcttga cggtttcgac ctcgcgcgag
     1921 atggtcgccg tacactcctg gacaattcgc tcaaattccg gagccaccgg atacttggcc
     1981 aattggcggg caacgcggcc ggccgacagc gcgatgggcg tcagcggatt cttgatctca
     2041 tgagctacgc gccgcgccac ttcgtgccag gccatggctt tttgtgcccg aagcagttcg
     2101 ctggtgtcct cgaccaccat cacaaagccg gcggtcgcgc gctcttccag cgccgatacg
     2161 gtcaaagaga gctgtagcgt ctgctggccg gttttgtagt cgagttggcg ggacgccaca
     2221 ccgagccgcc gcgcgcgttt catcaggtat cgcacttcgg cggcgtcgtc ccgggaaaac
     2281 agatcgtcga gcgtagtggc ccgcgccacc cgctcctcgg gcaggatgcg cttcaaggcc
     2341 gggttgtagc tttgaatgcg gccgtctcca ctcatggaga gcacgccggt gggaatgctg
     2401 tcgagaatgg tttccgtgaa ccggcggcgc cgttcgagct cgcggctgtt cgtttcgagc
     2461 gcctgcgtca tctcgttgaa cgttcgcacc agcgtcgcca gctcatcgat ggcattcacg
     2521 tgaacgcggt gagacaggtt gccgctgcgg atttcgcggg cggcctccag caggccggta
     2581 atgggcaggc tcagccgccg tgcaaagacg ccagccagcc acgtcgcaaa gaatagcagg
     2641 aagagcacaa tcagaaccag cagcgcccgg tagaagtaac gaaactcctt tttgttggcg
     2701 tgcaactggt tgtaatcggc gatctcgcgc cggatttcgt tttgttcccg ggcgatgtcc
     2761 acgggaatgc gatcccggac cagcaggttt gcaacgaaaa tgccggcggc ctggacaggt
     2821 gcgcgcgctt ccacgagagg agcgcgttgt gccagctcat ccggattaat gcggcagacc
     2881 ggcagagagg cgccggaagc agcttcgagc aaaacctcgt ccagcgcctt ttgcttgcag
     2941 atgctttcga aaacgttctg cggccggatg ccggtttccg cataagcctg gatctcgggc
     3001 atcgaagcga tctgcccggc ctgcgccgcg atgcgggcgc ggatctcctg ttcgagcgcg
     3061 ctgccgattt cagccaggtt gattttgatg ttttcagccg gccgcgtgaa ccacttgtcc
     3121 agattgcgat tcagaacgtg gatatcgaag atgaccagaa aaatgaccgg cacaaagctc
     3181 aaagtcagag cgccggcgat cagcttggtc ctgatccgcg agccttcgcg gttctcctgg
     3241 cgctccatgt acaacttgaa cgccgtgcgg aacagcatga atcccagcgt gacggtaagc
     3301 aggaagacca gtgtcgaaac cgcccagaac agatacgtct gctgcgggct ggaggggcca
     3361 tagttgccga aatcgaacga tccctgccac accaccaggg tgaccagggc gatcatcacg
     3421 gcgacaccgg cgctgttgat cagacgcttc ttcacggctg agagaacctt ctcaaattat
     3481 acgggacggc ttttagcggc agaaatggaa aaggggcgct cgttcggagc gcccctttga
     3541 gtaaaacagt ttggtctggc gatttagaag atgaacttta ccaggaagcg gccctggaag
     3601 cctggattga agatgccgtc cttgccgtag cgctgatcca gggctctagc tccgtcaggg
     3661 gttgcggcaa ccatggaccg ccagtcgaag cctctggaca gatccgtatt gctgaggttg
     3721 atgccggcgg agtctgagtg ttcttcacgg ttgtagcggt cgaacacata catactggtc
     3781 ttctggttga aaaggttatc catgttgaac tcgaaccgca gccgctttac ttctcccagc
     3841 cttacctcat gggctaccat caggtcggtc tggttgaaga atggtgttcg gccccagtca
     3901 ccccgcccct cgacgtagaa ggggatgtcg ttgacggtca caacctgtgt cgtaacggga
     3961 gttccactca tcatgcggaa gaaaccaccg atttctgtgc cgaatgagaa gtgcttcgca
     4021 ccgtagaact tgaagacatg cggtcggtcg gttggcaaac ggccgaatag gcctactctt
     4081 ccgttggcat cccacattgc ttcatcgaga tcgaagtaac ggtttgcatt tccgccggga
     4141 cggtagtttt ccgccgcgaa aacctggtta ggaccgaatc cataaccgag cgttggcggc
     4201 cggatctcgt ccgtgctctg caagccagtg tagttgcccc agagcctgct atatacgtag
     4261 ctcacattgc caagccaccc gttcccgaat cgcttagtca gcgagagttc catcgcatcg
     4321 tattcacgct ttgccttggg catctcaaaa ccgcaagctt ccccgacttg gacggtgcaa
     4381 gtggcgccgg aggccggaaa gaccgtattc gttccttcgc cggggttccc atagcgatag
     4441 acttcgttgc cgctagcatc cagagctccc atgtcctcga tagtgcggtt caagtggttc
     4501 cgcacgtacc gtccggcaaa aaccatctgc ggccggactt catactcgat gccgatgttc
     4561 atcacatcgg cactcatcgg tttcatgtcg ggatcaagat actcaaatcc gggcagccgc
     4621 cggtcgcggt agggacctga ccagagattc cggccgggca tattgttcaa gctgatgctg
     4681 taaatgtcga gcgtatcgag cgagcgataa tgctgttgcc acaggtcgcc accgaaggtg
     4741 ccacgagcca gatcgaactt tgtccagtcg tagaaccggc cccaggcacc cgaaatcttc
     4801 atttttccat cgccgaatac gtcgaagctt gcgccgaggc gtggagcgat cttatctccg
     4861 aatccgaact ggaaggcata ttcctgaaca tccctgcgga aggaaggaat ggtctccttt
     4921 tccgttcgga ggcccacatt caaagtcagc cgcgggtgta cgcgccacga atcctgaata
     4981 tacatgtgta tgatgctggc gccggtggag ccacgagtcg ctgcgtcgtc gacaatgtaa
     5041 taaccatacg tgccgcggtc gccgctggcg acggaaagat cccagtaaag ctgcacgcgt
     5101 ccgtccggtc ccacccaggc atcgtttacg ttattgacgt tcttctgcgt tccgacgccg
     5161 gccttcagat tgtgtgatcc caggaaccgc aggaactggc tgaaatcggc ctgaacgtag
     5221 gaacgcgtcg tgatatcgtg caccacctgc gcggcgctgg cggtcgcata attgaacggc
     5281 tgctgcaagt ctgccggaac gccgggaaga cctaccgacg aagcttgcca ccaatatgat
     5341 ttcaagaacg gcacgccgat ttctttgtag ttcaggtaat agcggcctcc tttaacactc
     5401 agcaaggaag tattccccag cgtgaaatca agattcccgg tatagctctg ctccggctgg
     5461 ttgtagccgc gagaagccga atcctgcgcc gcctggaggc tcctggtgct tacattcggg
     5521 gaaatgccgt cataagcgaa cagactgccg gttaaatact gcggtg
//
LOCUS       DASTMK010000439         8558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_149106, whole genome shotgun sequence.
ACCESSION   DASTMK010000439 DASTMK010000000
VERSION     DASTMK010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149106"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..563)
                     /locus_tag="VFQ79_18780"
     CDS             complement(21..563)
                     /locus_tag="VFQ79_18780"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0141775.1"
                     /translation="MPKRKPADDVLETMLRDGGRLLDTAAAIGLAAPPIKPPGGLRTR
                     VLSATRQASAEAQHGPQVWKNWPAPSPGSLHVVRQGEGSWENVRAGVMAKSLYVDPER
                     DIVTMLVSMQPGSTYVPHRHAGPEQCFVLEGDLHDGERVFRAGDFQCAETGSVHGTQW
                     TESGCLLLIVSSLNDELLSL"
     gene            complement(550..1188)
                     /locus_tag="VFQ79_18785"
     CDS             complement(550..1188)
                     /locus_tag="VFQ79_18785"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0141776.1"
                     /translation="MQNSPSRSDRTQDALRIFSRDQEWAGYVRRIAGGDDTALASLYD
                     QTSTLLYSIAVRILGNPADAEEVTLDVYTQIWNKAGSFDASRGSASAWIVMLARSRAL
                     DRLRSRAVQKRREEPLPESLRDTLASPAVEPSEYPDVDHRTLHAALGKLSPEQRQVIE
                     LAFFSGFTQSELAKHLALPLGTVKTRVRLAMLKLRKALESLPISMGRTNAEA"
     gene            1341..2021
                     /locus_tag="VFQ79_18790"
     CDS             1341..2021
                     /locus_tag="VFQ79_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0141777.1"
                     /translation="MRRVLLMMALGAGILDPGWGAPVACSPGTLDSYISLGTDGCTIG
                     DATVSDFASLPPLGEASEIAPGSIFVTPLLDGARFGLRFGFGATAAAGDILQSLIGFQ
                     VNASGLSSVEVSIAGAAASGDASVTAVPNLCLAGVFSGFDCAGTPAIAPIAFAIATDS
                     MLSARQTFSRSMSAGLLQDLVLDGGIAGNAALQSATLRFSAVPEPGTYITVFSGLLFA
                     ALLRLRRR"
     gene            2040..3578
                     /locus_tag="VFQ79_18795"
     CDS             2040..3578
                     /locus_tag="VFQ79_18795"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEU0141778.1"
                     /translation="MRKLPALAVSALAVFMAWDTTYGANHREAPITAFDHKADITDIY
                     AFRSYGGDTGKVTLILCVDPLLDPANGPNWFPFDPDILYEIKIDNNNDAVEDIVFEFR
                     FDTEQRLPGLFQVYAGVPGGANSPANSPPPVPPGTPIVPNRIVSFADAGLGQRQSYTV
                     TMVRNGSRTRISPGSPLYAVPVNVGPRTMDYEALFQAGIYSLANGVRVFAGTTDDAFW
                     IDLGGAFDTLNTSVPPVLSETQDAANQNFASDTVSGYAVNSIAIEVPTSMLTRTGNIE
                     AANSPAATIGIWGTTSRQRITVRRSPFPAQGAGSFLQVQRMGNPLINELLVGTGYKDR
                     FSMDQPKRDSQFANFFLDPTLARVVNALTNGAVAIPAPPRTDLLPVVTYAPPIAAPGT
                     PAGPVADLLRLNTGVPPTPFNNANRLGLLGNDPAGFPNGRRVFDDVTDIALRLVAGGV
                     LAGPPFSTSPVNSRLGDGVNVNDAPYRPDFPYLASSPSGRTRRHIDPGEAGCTAGTGA
                     PCAP"
     gene            3591..4724
                     /locus_tag="VFQ79_18800"
     CDS             3591..4724
                     /locus_tag="VFQ79_18800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141779.1"
                     /translation="MKPIIILLICASLWAGEQSPAEQAIAAATEALEKNPKRVESHNT
                     LAMALARRARETADTSYYDRAEAALAASLKIEPGNFEARKIQAWLLLGKHRFADALKL
                     STALNREAADDVMVYGLMADAHAELGNYSQAEEAVQWMLNIGRGSIPGLTRAAHLREL
                     FGDIQGALELMLTAYQRLSPSENEERAWVLTHMAHLELVNGRYEQASKLAGEALQLFP
                     DYHYALAQLAKVRASQGKAAEAAALLRKRYDAAPHPENLYELATALKAAGRNKEARES
                     FAEFEKAARAEMESWDNANRELIFYYTDHAGNPEEALRVARLEIARRRDVYTLDAYAW
                     ALYKNRKTAEARRQMETALAVGVRDPNILKHARVIGVELAEIH"
     gene            4756..5205
                     /locus_tag="VFQ79_18805"
     CDS             4756..5205
                     /locus_tag="VFQ79_18805"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEU0141780.1"
                     /translation="MELRARRKKQPTSIPPVEPERRRDPKIRRQMSGPAVRTYFNVAE
                     KWGLTVEEQRALLGWPPESTYYKYKAGQVGTLSYDMLMRISLVLGIYKNLHILYPEEA
                     LADGWVRLPNSNPLFGGKPALTLMTEGGMDGLYQVRRLLDGRRGGWN"
     gene            5196..5873
                     /locus_tag="VFQ79_18810"
     CDS             5196..5873
                     /locus_tag="VFQ79_18810"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU0141781.1"
                     /translation="MELTPPVTRLNLRDTCRLVPSLYPVAGILDTVASAEDLPYIFEL
                     EGWTNDRISTELGILHRLPKDEWVVGRAMASVIMAGFCHPRPEGGRFNGPDRGAWYAG
                     RTLETAHAEVAYHRTRELEEIGVLDTRLQMRLYLADFKASFHDVRPRIPENEPLHNPL
                     DYTAPQTLARDLLRAGSNGVIYRSVRHAGGECIACFRPRLVANVRPDAHFEYRWEGSR
                     VPRIRRL"
     gene            complement(5885..6868)
                     /locus_tag="VFQ79_18815"
     CDS             complement(5885..6868)
                     /locus_tag="VFQ79_18815"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEU0141782.1"
                     /translation="MQALKRSITRGAWIVLLAAVPAAAHMISMSTGTLNIEGTRARYE
                     LRIPAYELQHAGGQVPDLLAEIRFSSNGEDARMVEKTCSYENGGEEYVCRAQYVFKAS
                     PETIDIRCTLYRVTVPNHVHLLQAENDGRTGQAVLDVTFDTGRLDFAVPGAVSVAASA
                     FFSGSLRTLAGLGQLLFLATILVAAHTRRELWAMAAAVIGGQTLAAVLLSTLEWYPSQ
                     RFAEAAVALTIAYLALDVLLFPAARHRWMVVLILGVFHGLYFDVFLRTGAFSPVFFLA
                     GAAAMELALLALMALVFSLLTRWITTARPLVVCRSLLAVAGLTWFIVRLRG"
     gene            6924..8516
                     /locus_tag="VFQ79_18820"
     CDS             6924..8516
                     /locus_tag="VFQ79_18820"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU0141783.1"
                     /translation="MTHAAGEQMKQLVEKLHHLEDRLRAGGGPSKIEKQHAQGKLTAR
                     ERIAALIDPGTMFLEVGLLIAFDKYDGQAPAAGVVTGLTRIEGHPAVVVANDATVKAG
                     AWWPETITKILRAQEIAMRNRLPIVYLVDSAGVNLPLQDGIFPGQYGAARIFYYNSLM
                     RRKLQVPQIAAVMGPCIAGGAYLPALSDVIFMVEGTSFMGLGGPNLVKGATGQVTDAE
                     TLGGARMHTSVSGVAHYMAKDDSHCIKLIRQKFREFAPAEPPPRGAAPLKSAEGLYEI
                     LPSDHRLPYDMQQIIERIFDGGESLEFQPDYAPEMICANMRLCGRPVAVIANRRGFLK
                     TEERPRLGGIVYTESAQKVAYFVENAERNGTPLVYLQDVSGFMVGVEAESDAIIRAGA
                     AMVEAMACATVPKIVLTLNHASGAGYYAMAAQGFDPSFTFGWPTARIGVMEGDAAVQA
                     IHGPELAKLKERGEEIPAGLKAKIEATRADYELWLDARHAAARGYMDAIIDPLDTRRV
                     LSFALEAACYRSNRRHLVLENL"
BASE COUNT         1740 a         2600 c         2631 g         1587 t
ORIGIN      
        1 gagaagtaac tccacccctt ttatagggac agcaattcgt cgttaagcga agacacgatc
       61 agcaacaggc acccgctttc cgtccattgt gtgccgtgca cgctgcctgt ctcggcgcat
      121 tggaagtctc ccgctcgaaa aacccgctca ccgtcgtgca ggtctccttc gagaacaaag
      181 cattgttccg gtccggcgtg acggtgcgga acatacgtcg agccaggctg catactcacg
      241 agcatggtga cgatgtcgcg ctcgggatcc acatataggg atttggccat cacgccggcg
      301 cggacgtttt cccaactgcc ctcgccttga cggacaacgt gcagcgaacc gggcgagggc
      361 gccggccagt tcttccaaac ctgcggaccg tgttgcgcct ccgcgctcgc ctggcgagtc
      421 gcggagagca ctcttgtccg cagtcctccc ggcggtttaa tcggtggagc ggcgagcccg
      481 atggcggccg cggtatcaag caagcggccg ccatcgcgca gcatagtctc cagaacatca
      541 tccgcgggct tacgcttcgg cattggtacg ccccatggaa atcggtagac tctccagagc
      601 cttccttagt ttcagcatag cgagtctgac acgtgtcttg acagtgccga ggggcagtgc
      661 caggtgcttt gccagttccg attgagtgaa cccggagaaa aatgcaagtt cgatgacctg
      721 gcgctgctcg ggagaaagct tccccagcgc cgcatgcagc gtgcggtggt cgacgtccgg
      781 atattcggaa ggttccaccg ccggtgaagc aagcgtgtcc cgcaaggatt ccggcagagg
      841 ttcttcccgc cgcttttgaa ccgctctgga acgtaagcgg tccagagcgc ggctgcgcgc
      901 cagcatcacg atccacgccg aggcggaacc ccggctggca tcgaagctgc cggccttgtt
      961 ccaaatctgc gtatagacgt ccaacgtgac ttcctcggca tccgccgggt tgcccagtat
     1021 gcggaccgcg atgctataca gcagagtgct cgtctggtcg tataaactcg ccagcgcggt
     1081 atcgtctcct cccgcgatac gccggacata gccggcccat tcctggtccc gggagaagat
     1141 ccgtaacgca tcctgcgttc tgtcggatcg cgacggagaa ttttgcatcg gcgaccgctg
     1201 cctgaccttc ccgattgtat cagcagagtt cacgattgcg gcctcgaatt ccaactggcg
     1261 tcatgaatcc ggcggcaatg agcggctcgt atactcgaac gtcagacgct cttagggaaa
     1321 tacggaagga gagaaatgta atgcgccgtg ttttgctgat gatggcactg ggcgccggga
     1381 tcctcgatcc gggctggggc gcgcctgttg cgtgctcacc gggaaccctc gacagctata
     1441 taagtctcgg aacggacggc tgcacaatcg gagacgcgac cgtctcggac ttcgcgtccc
     1501 tgccgccact gggagaggca agtgaaatcg cgccaggctc tatcttcgtg acgccgctac
     1561 tcgatggcgc ccggttcggt ttgcggttcg gctttggcgc aaccgcggcg gccggagaca
     1621 tactgcaatc gctgatcggg tttcaggtca acgcctccgg gttgtcgagc gtggaggtct
     1681 caatcgccgg cgcagcggct agcggggatg cgtccgtgac ggcggtgccc aatctttgtc
     1741 tcgcgggcgt attcagcgga ttcgattgtg cgggaacccc tgcgatcgcc ccgatcgcgt
     1801 tcgcgattgc cacggattca atgctttcgg cgcggcagac cttttcacgt tcgatgagcg
     1861 ccggcttgct ccaggacctg gtcctggatg ggggcattgc aggcaacgcc gctcttcaaa
     1921 gtgcaaccct ccgcttttcg gcggtgcctg agcccggaac ttatatcacg gtcttttccg
     1981 gactgctttt tgcagcttta ctgcgcctgc gaaggcgttg actcaacgag gagaacccaa
     2041 tgagaaaatt acccgctctg gctgtgtccg cgctcgcggt attcatggct tgggatacaa
     2101 cctatggcgc caatcaccgg gaggcgccca tcacggcgtt cgatcataag gcggacatca
     2161 cggacattta tgcgttccgc agctacggag gcgacaccgg caaggtgacg ctgattctgt
     2221 gcgtggatcc tctgctcgat ccggcgaatg gccccaattg gtttccattc gatccggaca
     2281 ttctctacga aatcaagatc gacaacaaca acgatgcggt ggaggacatc gtgttcgagt
     2341 ttcgcttcga cacggagcag cgtctgcctg gcctttttca ggtctacgcg ggagtgcccg
     2401 gcggagcgaa tagccccgct aattctccac cgcccgtgcc gcccggaacg cccatcgttc
     2461 ccaatcgcat cgtatctttc gccgacgccg gcctcggcca gagacagagc tataccgtca
     2521 caatggtcag gaacggatcg cggacgcgca ttagcccggg gagtccgcta tatgccgtgc
     2581 cggtgaatgt gggcccccgc actatggact atgaggcgtt gtttcaagcg ggaatctact
     2641 cgcttgcaaa tggagtccgc gtgtttgccg gaaccacaga cgacgcgttc tggatcgatc
     2701 tcggtggcgc cttcgatacc ttgaacacct cggttccgcc tgtcttgtcg gagacgcagg
     2761 atgcggctaa ccagaatttc gcgagcgata ccgtctccgg ctacgcggtg aactccatcg
     2821 cgatcgaagt gccgacctcc atgttgacga ggaccggcaa catcgaggcg gcgaactcgc
     2881 ccgccgccac gatcgggatt tggggaacga cctcccggca gcgcatcacg gtccgccgtt
     2941 ctccgttccc ggctcaaggc gcgggtagct tcttgcaggt ccagcgaatg ggcaatcccc
     3001 tgatcaacga actgctggtg ggaaccggct acaaggatcg cttcagcatg gatcagccga
     3061 agagggactc gcaattcgcc aacttcttcc tcgatccgac cctggcgcgc gtcgtgaacg
     3121 cgctcaccaa cggcgccgta gcaataccgg cgcctccgcg aacggacctg cttcccgtgg
     3181 ttacctatgc gcctccgatt gccgcgccgg gcacaccggc aggcccggtt gcggatctac
     3241 tcaggcttaa cacgggagtg cctcccacgc cgttcaacaa cgcaaaccgc ctgggtctgc
     3301 tcgggaacga tcccgcgggg ttcccgaacg gaagaagggt gttcgacgac gtgaccgata
     3361 tcgcgctccg tctggtggcc ggtggagtcc tggcgggtcc ccctttctcc acctcgccgg
     3421 tcaattccag gctgggcgat ggcgtgaacg tcaacgacgc gccatatagg ccggacttcc
     3481 cctatcttgc aagttcgccc agtgggcgca cccggagaca catcgatccc ggcgaggccg
     3541 gctgcacggc cggaaccgga gcgccctgcg caccctgatg tggaggcgaa atgaaaccaa
     3601 tcataatcct tctgatctgc gccagcctgt gggccggaga gcaatctccg gccgaacagg
     3661 ccatagctgc cgctacggaa gcgctggaaa agaaccccaa acgggtggaa agccacaata
     3721 ccctggcaat ggctttagcc cggcgcgccc gggagaccgc cgacacgtcc tattacgatc
     3781 gggcggaggc ggcattggcc gcttccctga agatcgagcc cggaaacttc gaagcacgca
     3841 aaatccaggc ctggctgttg cttggcaagc accgcttcgc cgatgccttg aaactgtcaa
     3901 cagctctgaa tcgcgaggcg gccgacgacg tgatggttta cgggttgatg gcggatgccc
     3961 atgccgagct cggcaattac tcgcaagccg aggaagccgt ccagtggatg ttaaacattg
     4021 gacggggcag cattccggga ttgacgagag ccgcgcatct gcgtgagctg ttcggggaca
     4081 tccagggcgc gctcgaactg atgttgaccg cctaccagcg gttaagtccg agcgagaacg
     4141 aagagcgtgc atgggtgctg acccatatgg cccacctgga gctggtgaac ggccgctacg
     4201 aacaggcttc taaacttgcc ggagaagcct tgcaactgtt cccggattac cattacgcgc
     4261 tggcgcagtt ggccaaggtg cgcgcgagcc aggggaaggc cgccgaagcg gcggccctgc
     4321 tcaggaagag atacgatgcg gcgccgcatc cggagaattt gtacgaattg gccaccgccc
     4381 tgaaagcggc cggccggaac aaggaagcgc gtgagagttt tgccgaattt gagaaagcgg
     4441 ctcgggccga aatggagtcc tgggataatg ccaaccgcga actgatcttc tactacacgg
     4501 accacgcggg caacccggaa gaagccctgc gcgtggccag gctggaaatc gcacgccggc
     4561 gcgacgtcta cacactcgac gcttatgcct gggcgctgta taagaatcgg aaaacggccg
     4621 aagcacgccg gcagatggag acggcattgg ctgtgggcgt ccgcgatccc aatatcctga
     4681 aacacgcgcg ggtgatcgga gtggaactcg cagaaattca ttgacggttc tcgaaatgta
     4741 gcgtaagctc cagatatgga gctccgcgcg cggcgcaaaa agcaacccac ctccatcccc
     4801 ccggtcgagc cggagcgcag acgcgatccg aaaatccggc gccagatgtc gggacccgcc
     4861 gtccgcacat atttcaacgt tgcggaaaag tgggggctca cggtggagga gcagcgggcc
     4921 ttgctcggct ggccgccgga atcgacctac tacaagtaca aggctggcca ggtgggaacg
     4981 ctgtcctatg atatgctgat gcgcatatcg ctggtcctcg ggatttacaa gaacctgcac
     5041 atcctttatc cggaagaggc gctggccgat ggatgggtgc ggctgccgaa cagcaatcca
     5101 ttgttcgggg gcaagccggc attgactctc atgacggaag gcgggatgga cgggctgtac
     5161 caggtgcgcc gtctgctcga cggccgccgc ggcggatgga attgacaccg ccggtcacgc
     5221 ggctgaacct gcgcgatacc tgccgcctgg ttccgagcct ttacccggtt gccggcatcc
     5281 tggacacggt tgcttcggcg gaagacctgc cgtatatctt cgaacttgaa ggctggacga
     5341 acgaccgcat ttccacggag ctcggaattc tgcatcgtct tccaaaagat gaatgggttg
     5401 tagggcgggc catggccagt gtgattatgg cggggttctg ccatccgcgc ccggaaggcg
     5461 ggcggttcaa tggaccggac cgtggagcgt ggtacgccgg cagaacactc gaaacggcgc
     5521 atgccgaggt tgcctatcat cgaacccgcg agctcgaaga gattggagtt ctcgataccc
     5581 ggctgcagat gaggctctac ctcgccgact ttaaggcgtc tttccacgat gtacggccgc
     5641 gcattccgga aaacgaaccg ctgcacaacc ccctggacta caccgcgccg caaacgctgg
     5701 cgcgcgatct gcttcgagcg ggttccaacg gagtaattta ccgcagcgtt cgccacgccg
     5761 gtggtgaatg cattgcgtgt ttccggcctc gattggtcgc caatgtccgt ccggatgccc
     5821 atttcgaata ccgctgggaa ggaagccggg ttcctaggat ccggcggctc tgagggccac
     5881 tccttcagcc cctcagcctg acgataaacc aggtgagacc ggcaacggcc agcaaagacc
     5941 ggcacaccac tagtggccgc gcggtggtaa tccaccgcgt gaggaggctg aaaaccaatg
     6001 ccataagcgc gagcagcgcc agctccatcg ccgccgcacc ggccagaaag aacaccggag
     6061 agaaagcacc ggtgcgaaga aatacgtcga aataaaggcc gtgaaaaacg ccgagtatca
     6121 gcactaccat ccagcggtgc ctggccgcgg gaaagagcag aacgtccaat gcgagatacg
     6181 caatggtcag cgcgactgcg gcttcggcga atctctggga tggataccac tcgagcgtgg
     6241 atagcagcac ggccgccagt gtctggcctc ctattacggc agcggccatc gcccacaact
     6301 cgcgccgcgt gtgcgctgcc acaaggatgg ttgccaggaa cagcagttgg cctaatcccg
     6361 ccagggtacg caggctgccc gaaaagaagg ctgaagccgc aaccgaaacg gcacccggca
     6421 cggcgaaatc aagccgcccg gtgtcgaaag tcacgtccaa caccgcctga ccggtccggc
     6481 cgtcgttttc agcctgcaac aagtgcacgt gattcggaac ggtcacccgg tagagagtgc
     6541 accggatatc gatggtctcg ggcgaagcct tgaaaacata ctgtgcccgg cacacgtact
     6601 cctcgccgcc gttctcgtac gagcacgtct tctctaccat tctggcgtct tcgccgttac
     6661 ttgaaaaccg gatctccgcc agcagatccg gaacctggcc gccagcgtgt tgaagttcgt
     6721 aagcgggtat gcgcaactcg taacgcgcgc gtgtcccctc gatgttcaag gtcccggtgc
     6781 tcatgctgat catgtgcgcc gcggcgggca cggctgcgag aagaacgatc cacgctccgc
     6841 gagtaatgct ccgtttcaga gcctgcatga ggggcatcct agcgcactat actaaacaca
     6901 gtgcaagtag tactcctgct gacatgactc acgctgctgg ggagcagatg aagcagcttg
     6961 ttgaaaagct gcaccatctg gaagaccgtc tgcgcgccgg gggcgggccg tcgaaaatcg
     7021 aaaaacaaca tgcgcagggt aagctcacgg ccagggaacg aattgccgcg ctcatcgacc
     7081 cgggcacgat gtttctcgaa gtgggcttgc tgatcgcatt cgacaagtat gacggacagg
     7141 ctcctgccgc gggcgttgtc acgggcctga cgcgcatcga aggccatccg gcggtggtcg
     7201 tcgccaacga cgctacggtg aaggccggcg catggtggcc ggagacgatc acgaaaattc
     7261 tgcgcgcgca ggagattgcc atgcgcaacc gccttccgat tgtctacctg gtggactcgg
     7321 caggcgtcaa tctcccattg caggacggta tcttccccgg ccagtatggc gccgcccgga
     7381 tcttctacta caactcgctc atgcggcgaa aactccaggt tcctcagatt gcggccgtca
     7441 tgggtccctg catcgccgga ggggcgtatt taccggcact gagcgacgtg attttcatgg
     7501 tggagggcac cagttttatg gggctcggcg gaccgaacct ggtgaaaggt gcgacgggtc
     7561 aggtaacgga cgccgaaacg ctgggcggcg cgcggatgca caccagcgtg agcggtgtcg
     7621 cgcattacat ggcgaaggac gattcgcatt gcataaaact cattcgccag aaattccggg
     7681 agtttgcgcc ggcagagccc ccgccgcgag gcgccgcccc cctgaaatcc gcggaaggcc
     7741 tgtacgaaat cctgccgtcg gatcaccggc ttccctacga catgcagcag atcatcgaac
     7801 gcattttcga cggcggtgaa tccctggaat tccagccgga ctatgcaccc gagatgatat
     7861 gcgccaacat gaggctgtgc ggccggcctg tcgccgtcat cgccaaccgg cgcggattcc
     7921 tgaagacgga agaacggccg cggctgggtg gtatcgtgta cacggagtcg gctcaaaagg
     7981 tggcctattt tgtcgaaaac gcggaacgca acggaacacc gctcgtctac ctccaggatg
     8041 tttcagggtt catggtggga gtggaagccg aaagcgacgc cattattcgt gcgggcgccg
     8101 cgatggtgga agccatggcc tgcgcgaccg ttccgaaaat tgtgctgact ctcaaccatg
     8161 ccagcggagc gggttactat gccatggcgg cccagggctt cgacccgagc tttacgttcg
     8221 gatggcccac ggctcgaatc ggagtcatgg aaggcgatgc tgccgtgcag gccatccatg
     8281 gaccggagtt ggcgaagctt aaagagcgcg gtgaagagat tcccgccggc ttgaaagcga
     8341 agatagaggc cacgagagcg gattacgaac tctggctcga tgcgcggcac gccgccgccc
     8401 ggggatacat ggacgcgatc attgacccgc tcgacaccag gcgtgttctc tcgttcgcgc
     8461 tcgaagccgc ctgctaccga agcaaccggc gccatctcgt gctggagaac ttatgatccg
     8521 aattgcaaac ggccagggct tttggggaga ctggctgg
//
LOCUS       DASTMK010000440         4976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_23928, whole genome shotgun sequence.
ACCESSION   DASTMK010000440 DASTMK010000000
VERSION     DASTMK010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23928"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VFQ79_18825"
     CDS             <1..1000
                     /locus_tag="VFQ79_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141784.1"
                     /translation="EVLRGPLSLTSRRWGFPYSTVNPLFSPARMNTFVEYRFYAGTPW
                     FLKTGYMQVLKEMEIGYLRDDEWVFSGMSFTDILWMGADGKLKTGPVDKGSAENLWSV
                     GFFNRDTRDAFIGVFLEHTAENVGGLKHTGAPLFYYKWHGHVWSRALYHNATLPAGAV
                     LRQKNAYLTLPFPAGQGAEMVERFRHQLLNPLLPSPAQLPSGMSAGKPAGRLARPGEA
                     GDSSISKKLLWDALAECQDQQLYTSRPSVVDLGLVYDLRVRGDVVFVLMAMPHRGRPR
                     IGYFSYGSGGNSTPVEQMLLKVPGVRKVIVEQTWEPHWDANRVTAEGRRKLGLPEI"
     gene            complement(1022..2035)
                     /locus_tag="VFQ79_18830"
     CDS             complement(1022..2035)
                     /locus_tag="VFQ79_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141785.1"
                     /translation="MVQLFGGLKVGAIVCSTRSTVEDPFYPGKAAGDVYDNRFKESHS
                     IPFLPEHVPQGLAAWSNWSHGSGEDDLLLITSYDPAERYSLIIGIDAKTGKRIGAAKI
                     AYSHVGGIAVFEKLGWAFVSDEEPRKVRRYSLKKLADAISTSDKKILDSEPPDQSVIG
                     ASFLTSHGPTNTLWAGNFKQSAKGGRSRMKAYKVDKNGQLKPENGSWEVPIKTQGVVV
                     TKDFLIYSTSFGRNNRSNLYVVRRGKGETDLDNARLFCFRSPSMSEGIAVYGDNLYVL
                     YESGARIYNPPIAPGSPKPDVPRNKISNLHRAPLSSLEELPPRSGPSRPAPIGPDKRK
                     LKR"
     gene            complement(2087..3157)
                     /locus_tag="VFQ79_18835"
     CDS             complement(2087..3157)
                     /locus_tag="VFQ79_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141786.1"
                     /translation="MALWQIAGVVLILLLVQFAGWPSSSAPAEVPGIPALLQEPGYFI
                     ETAGGKWRVRKPGIRIFDPVGSKPAEAWTVFEDTRQDMVEWRKEALPKLRLNRALVRF
                     DPITRRRTLVEDWTRESDALEIEADVRALLERFAAPDVLALFSSTPLYMARLNGASGH
                     TAEEWNDQQRFVIYIDPFRATGRLHAASTLVHELSHVAGYRKRGFHANRAVSVLTRSD
                     FILLGVSDEFAAYEAEAAFIESFVNSVRSDDDRGTIARLMTLPELRWPAGLIELLGFG
                     RSPEAGARRGEARKHVVLDLDRQAARYWDAYHKRELSASVEGAIRDWYVHSQEWKEIA
                     GKRDEWLSAGACVDEAAPSGTR"
     gene            3362..>4976
                     /locus_tag="VFQ79_18840"
     CDS             3362..>4976
                     /locus_tag="VFQ79_18840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141787.1"
                     /translation="MRKLISFWRSCLLLTACIGLCLAQDYRARVQGNVTDQTQAAVAG
                     AKVTLTNQDTGVELSRLTDGSGRFLFDYVAPGTYTVRMEMAGFSPFSQENITVLTRGD
                     VTVNGTLSVGGITQTVNVSESVMALEFNTSTMSQTVTGKMLQDLPILARNPFTLVLLD
                     PAVVNRYSTVAHRNPFYMQAANGVDVGGATGGRNDILLDGVPIGVDSRGSYSPSMDSV
                     QEVSIQQNSVDAEFGFSAGGNMSVSMKSGTNEYHGTVYYFGRNPALNAMANRYTRSEN
                     LVRQHIGGGTVGGPILKNRLFTFFSYERWNKTEPREGTRTLPTDLERTGDFSRSLNAA
                     GGLRTIYDPWSTQFDAATSTASRTPFPNNIIPNSRIDPAAAIVMGDLWKPNGPGDDIT
                     GTNNFKITYPWWTKYYNLSNRTDWNVNDKFRMYARYSIFRTRLDNNNYGNSPATPSDN
                     GGLMDSLNAAADGVYTMNPSTVLNVRFGVTYLEDDYDSDWAKVGEAGLQKVWPSTPWY
                     TSYIQGIPAIYYPAISVSGKGSFGKGSTWF"
BASE COUNT         1008 a         1515 c         1402 g         1051 t
ORIGIN      
        1 tgaggtcctg cgcggtccac tctctctcac cagtcgccgc tgggggtttc catactccac
       61 tgtgaatcct ctcttctcac ccgcccgcat gaacacgttc gtcgaatacc gtttttatgc
      121 cggcacaccc tggttcctca aaaccggtta catgcaggtg ctcaaagaga tggagatcgg
      181 atatctgcgg gacgatgagt gggtcttctc cggtatgtcg ttcaccgaca ttttgtggat
      241 gggcgcggac ggcaagttga agaccggccc ggtggacaaa ggatccgcgg agaatctctg
      301 gtcggtcggc tttttcaatc gcgatacccg cgacgccttt atcggagtgt tcctggagca
      361 tacggcggag aacgtgggcg gcttgaaaca tactggcgcg ccattgtttt attacaagtg
      421 gcacggacat gtctggagcc gcgcgctgta tcacaatgcg acgcttcccg ccggggccgt
      481 tttgcgccag aagaacgctt atctgacgct gccgtttcct gccgggcaag gcgcggaaat
      541 ggtggagcga ttccgtcacc aactcctgaa tcctctgctt ccctcccctg ctcaattgcc
      601 atccggcatg tcggcaggca agcccgccgg acgtctcgcc cgccccggtg aggccggaga
      661 cagctcgata tccaagaagc tgctgtggga cgcgctcgcg gaatgccagg accagcaact
      721 gtatacgtcg cggcccagcg tcgtggatct cgggctggtg tacgacctgc gcgtccgcgg
      781 agatgtggtt ttcgtgttaa tggccatgcc gcaccgcggg cgcccccgca tcggctattt
      841 ctcatacggc tccggcggca actcgacgcc cgttgaacag atgctgctca aggttcccgg
      901 agtgcgcaag gtgatcgtcg agcagacgtg ggaacctcat tgggacgcga atcgcgtgac
      961 cgccgagggg cgccgcaaac tgggtctccc ggaaatttaa cgcaagtgct ggacaccacc
     1021 cctagcgttt taatttgcgc ttgtcgggcc ctatcggtgc cgggcgtgat gggccggagc
     1081 gcggcggtag ttcctccagt gacgacagcg gtgccctgtg caagtttgag atcttgttgc
     1141 gcggaacgtc cggctttggg ctcccaggtg cgatgggcgg gttgtatatc ctggcacccg
     1201 actcgtagag cacatacaaa ttgtcgccat agacggcgat gccttcggac atgcttgggc
     1261 tccggaagca gaacagccgc gcgttatcga gatccgtctc accctttcca cgccggacga
     1321 catacagatt gctgcggttg ttgcggccga atgaagtgct gtagatcaag aagtccttgg
     1381 tgacgaccac cccctgcgtc ttgatcggca cttcccaact gccgttctca ggcttcaact
     1441 ggccgttctt gtcgaccttg tacgctttca tgcgggaccg gccacccttg gccgattgct
     1501 tgaaattgcc cgcccatagc gtattggtcg gtccgtgact ggtcaggaag gacgcaccga
     1561 tgacactctg gtccggcggc tccgaatcga ggatcttctt atctgacgtc gagatcgcgt
     1621 ccgccagctt tttcagagag tacctacgta ctttgcgggg ctcttcatcc gacacaaacg
     1681 cccacccgag cttttcaaag accgctatcc cgccaacgtg cgagtaggcg attttcgcgg
     1741 cgccgattcg tttgcctgtc ttcgcatcga tgccgatgat cagggagtac cgttccgccg
     1801 ggtcatacga cgtgatgagc aggagatcgt cctcgcccga accatgtgac cagttcgacc
     1861 aggccgccaa gccctgaggc acgtgttccg gcagaaaagg aatgctgtgg ctctctttga
     1921 aacggttgtc gtagacatct cctgccgcct ttcccggata gaagggatct tcgacggttg
     1981 aacgcgtact acagacgatg gcgcccactt tgagtcctcc aaacagttgg accaacgcag
     2041 ggcctgttaa gtgacatgct atttcggcca ggatttccgg cgcggattat cgagttccgg
     2101 acggcgcggc ctcatcgacg caggcgccgg cagacagcca ttcgtcccgc ttgccggcta
     2161 tctctttcca ttcctgcgaa tgcacgtacc agtcgcgaat cgccccctcg acggaagcgc
     2221 tcagctcgcg cttatgatag gcgtcccaat accgtgcagc ctgacggtcg agatccaata
     2281 caacgtgctt gcgcgcttcc ccccgccgcg cgcctgcttc cggcgagcgt ccgaagccta
     2341 ataattctat gagcccggcc ggccacctca attccggcaa cgtcatcaac cgggcaatgg
     2401 tcccgcgatc gtcgtcgctc cgaacgctgt ttacaaacga ttcgatgaag gcggcttccg
     2461 cctcgtacgc cgcaaactca tcggagacgc caagcaggat gaagtcgctt ctggtcagga
     2521 ctgagacggc acggttcgca tgaaatccgc gcttccggta accggcgaca tgcgagagct
     2581 cgtgcaccag cgtcgaggcg gcatgcagcc ggccggtggc ccggaaagga tcgatataaa
     2641 ttacgaaccg ctgttgatcg ttccactctt ccgccgtatg tccggacgcg ccattcaagc
     2701 gcgccatata gagcggggtt gacgaaaata gcgccagcac atccggcgct gcgaacctct
     2761 cgagaagtgc gcggacgtcg gcttcaattt ccagggcatc gctttcccgg gtccaatcct
     2821 ctaccagcgt tcgccgccgc gtaatgggat cgaaccgcac cagtgcccga ttcagccgca
     2881 gtttcggcag ggcctccttg cgccactcca ccatgtcttg ccgggtatcc tcgaagaccg
     2941 tccaggcttc cgccggtttg cttcccactg gatcgaagat tcgaatgccg ggttttctca
     3001 ctctccattt gccgccagcc gtttcgatga aatagccggg ctcctggaga agcgccggta
     3061 taccgggaac ttccgcgggc gcagaagatg atggccaccc tgcaaactgc accagcagca
     3121 aaatcagaac cacgcccgca atttgccata gcgccatgcc gttcctacta aggtagaacg
     3181 gttcgcggtg tacctgcccc ggacgcggaa atctgcctga aaagacccaa aacactaaat
     3241 ccttgactct cccccaaggc gcctatataa tcttccaaac cgtctgaacc gtgctggcat
     3301 tggggattgg gggcaatcgc cattatggag acgcgtatca aacacttaaa ggggagttgc
     3361 aatgagaaag ctgatttctt tctggcgttc ctgcttgttg ctcaccgcat gtataggtct
     3421 ctgtttggca caggactatc gtgcgcgcgt gcagggtaac gtcacggatc aaactcaggc
     3481 ggcggtcgcc ggcgccaaag taactttgac aaaccaggac acaggcgttg aactctcgcg
     3541 ccttaccgat ggcagtggac gttttctgtt cgactacgtg gcgccgggca cttacaccgt
     3601 tcgtatggag atggccggtt tcagcccgtt ttcgcaggag aatattactg ttcttactcg
     3661 cggagacgtc accgtaaacg gcacgctcag cgtcggcggc atcactcaga ccgtcaatgt
     3721 ctccgagtcc gtaatggcgc tggaattcaa cacctccacc atgtcacaga cggtcaccgg
     3781 aaaaatgctt caggatctcc cgatcctcgc gcgaaatcca ttcaccctgg tgttgctcga
     3841 cccagccgtg gtgaaccgct attcgacggt ggcccaccgg aatccgtttt acatgcaggc
     3901 tgccaatgga gtggatgtag gcggcgccac gggcgggcgg aatgacatac tgctcgacgg
     3961 tgtgcccatc ggtgtggact cgcgcggatc gtattcgccc tcgatggatt ccgtgcagga
     4021 agtttctatc cagcagaaca gcgtggatgc cgaattcggc ttcagcgccg gtggaaacat
     4081 gagcgtgtcc atgaaatccg gcacgaacga atatcacggc acagtttact acttcggccg
     4141 taacccggcg ctcaatgcca tggccaaccg gtacacacgg agcgagaacc tggtccgcca
     4201 gcacatcggc ggcggtaccg tcggtggacc cattctcaag aacagactgt tcactttctt
     4261 ctcttatgag aggtggaaca aaacagagcc gcgggaagga acgcgaaccc tgccaaccga
     4321 cctcgagcgc accggcgatt tctcccggtc tctgaatgcg gcgggcgggc tgcgcactat
     4381 ttacgatccg tggtcgacgc agtttgatgc tgctacctca acggcctccc gaactccatt
     4441 tccgaacaac atcattccaa acagccgcat cgacccggcg gcggcgatcg tcatgggcga
     4501 tttgtggaag ccgaatggac cgggtgacga catcaccggc accaataact tcaagatcac
     4561 ctacccgtgg tggacgaagt attacaacct ttcgaaccgt acggactgga atgtgaacga
     4621 taagtttcgt atgtatgccc ggtatagcat cttccgcacc cggcttgaca acaacaacta
     4681 cggaaactcc cccgccactc catcggacaa tggcggcctg atggactcgc tcaacgcggc
     4741 cgcggatggc gtttacacga tgaatccctc cacggtgctg aacgtgcggt ttggagtgac
     4801 gtatctcgaa gacgactacg attcggactg ggccaaggtt ggcgaagcgg gcctccagaa
     4861 agtctggccg agcactccct ggtacacgtc ctacatccag ggcattccgg cgatttacta
     4921 tcccgccatc agcgtttccg gcaaaggaag cttcggcaag ggtagcacct ggtttt
//
LOCUS       DASTMK010000441         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_24077, whole genome shotgun sequence.
ACCESSION   DASTMK010000441 DASTMK010000000
VERSION     DASTMK010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24077"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..385
                     /locus_tag="VFQ79_18845"
     CDS             74..385
                     /locus_tag="VFQ79_18845"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HEU0141788.1"
                     /translation="MLQLNGKSVPARTRREPGRAETLMELREGEHGILEALEVPEDIA
                     QRLMELGFIPGSRIVAARSAPGGDPRVFQVDGSEIALRLETAAALRLRAFPAAQPSDV
                     S"
     gene            382..2277
                     /locus_tag="VFQ79_18850"
     CDS             382..2277
                     /locus_tag="VFQ79_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEU0141789.1"
                     /translation="MSGCHDCSGGGIGTVVAPVAANRTKVVAIVGPPNSGKSTLFNRL
                     TGLRQKVANFPGVTVEQHLGKARLGGGREVMLVDLPGIYSMTPRSEDEQVTHDVLKGR
                     MKNVARPDAVLLILDSTNLGRHLTLAAPVLSLGVPTLVILNMADDLRHRGGNVDTAAL
                     SQQLGAPVVLVSASQGEGIEAVFSFLEKGTSAPVPVELPIIQDVPRCRAWAARIGTKA
                     AYQAPAPPLWTRRLDAIFLHPVMGPVFFAIVVVAVFQTIFSGAKPLMEGVESLIAASG
                     DWVASVLPESAFRSLLIDGVWGGVGSVVIFLPQILLLFLFIGILEDSGYLARAALIAD
                     RTMARVGLQGKSFIPLLSAYACAVPAIMATRTIENKRDRYATILIAPFMTCSARLPVY
                     TLIIAAFIPNHSLLGPLLGTQAAAMLGLYLLGFLAAFVTARVLKSSVLKSERTPFMLE
                     MPPYRKPTLQGLALRLLDRAKVFLRRAGTVILAVAILLWVLANIPLHDGSAPPIEQSV
                     AGTVGRAVEPLIRPLGFDWKIGIGLITSLAAREVIVGTLGTIYGIEGEADSKGLQQAL
                     QRDLDLPGAVALLVFFAFAMQCMSTLAVVRRETGGWKWPVLQFTYMSALAYVSAFVAN
                     RAVAAFV"
     gene            complement(2286..2648)
                     /locus_tag="VFQ79_18855"
     CDS             complement(2286..2648)
                     /locus_tag="VFQ79_18855"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0141790.1"
                     /translation="MTVFTALAIGLVAGVFSGMFGIGGGLIIVPALVILMKMEPLGAI
                     GTSLAALIPPVSLLGAIEYHRAGHMNLKYAMLIALGLFLGAYFGARILLGLPPALVRK
                     LYGIFLLVIATRMLFFQK"
     gene            2720..>3927
                     /locus_tag="VFQ79_18860"
     CDS             2720..>3927
                     /locus_tag="VFQ79_18860"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0141791.1"
                     /translation="MKLHSYTITQGRDRAPARAMLKAIGFTDEDLRKPVIGVANTWIE
                     TMPCNYNLRDLAVKVKDGIRAAGGTPMEFNTIAISDGVTMGTEGMKSSLVSRDVIADS
                     IELVARGHMFDGIVALVACDKTIPGAAMALLRLNVPGLVLYGGSILPGHYNGRDLTIQ
                     DVFEAVGANASGRISDQELLAIENAACPGAGACGGQFTANTMATLMEVIGLSPLGTAS
                     VPQVDPRKNDVAERCGRVIMDAVKLDLKPRDIVTRKAFENAIASVAATGGSTNSVLHL
                     LAMAREAGVDLKIDDFQTVSSRTPLLVDLKPAGHFVAVDVDKAGGIPVIAQRLVEGGY
                     ADGSAITITGRTLEAEAAEAKETPGQEVIHPLSNPIKKSGGLVILKGSLAPEGCVIKV
                     TGIERKGHTGP"
BASE COUNT          765 a         1158 c         1210 g          794 t
ORIGIN      
        1 atatgcaagt gaatgtctgt taaggtcata gcgtatcttg acagcatcgg tcacgagatt
       61 ggatactgga actgtgctgc aactgaatgg caagagtgtt ccggcacgaa cccgtcgtga
      121 gccgggtcgc gctgaaaccc tcatggagct tcgcgagggt gaacacggca tcctggaagc
      181 tttggaagta cctgaagata tcgcacaaag gctgatggaa ttggggttta tccccggcag
      241 ccggatcgtg gctgccagga gcgcgccagg cggggaccca agagtgtttc aggtcgatgg
      301 atctgaaatt gccctccggc tggagaccgc ggcggctctg cggctgcgag ctttcccggc
      361 ggcgcagccg tccgacgtct catgagcggc tgccacgatt gctctggcgg cggcattggc
      421 acagtggttg cgccggttgc agcgaaccgc acgaaagtag tcgccatagt aggccctccg
      481 aattcaggca aatccaccct cttcaaccgt cttacgggtc tgcggcaaaa ggtcgcgaac
      541 ttccccggcg ttactgtcga acaacatttg ggaaaagcca ggctcggcgg cgggcgcgaa
      601 gtgatgctgg tggatttacc cggcatctac tcgatgacgc cgcggtccga ggacgaacag
      661 gtaacacatg acgttctgaa gggcagaatg aaaaacgtgg cgcggccgga tgccgtgctg
      721 ctcattctgg attcgaccaa tctcgggcgg cacctgactc tggccgctcc cgttctgtct
      781 ctcggcgttc ccaccctggt cattctgaac atggctgacg atcttcgcca ccggggtgga
      841 aacgtggaca ccgctgcact gtcgcagcaa ctaggcgcgc ccgtcgttct cgtcagtgca
      901 tcgcagggcg aaggaataga ggccgtcttt tcgtttctgg agaaggggac ctccgcaccg
      961 gttcctgtcg aactgcccat tatccaggac gttccccgtt gccgggcttg ggctgcgaga
     1021 atcggcacca aagcggcata ccaggcgccc gcgcctcctc tttggactcg ccgtctggat
     1081 gcgatatttt tgcacccggt aatgggtccg gtgttcttcg cgatcgttgt ggtcgcggtt
     1141 tttcaaacga ttttctcggg cgccaaaccg ctgatggaag gcgtggagag cctgattgcc
     1201 gcgtccggcg attgggtggc ttccgtgctt cccgagtccg ccttccgctc gctgttaatt
     1261 gacggcgtgt ggggcggcgt cggctctgta gtcatatttc tgccgcagat cctgctgctg
     1321 tttctgttca tcggcattct ggaagattcg ggctatctcg cacgggccgc attgattgcg
     1381 gatcgcacca tggcccgggt cggtctgcag ggcaagtcgt tcattcccct gctgtcggcc
     1441 tatgcctgcg ccgtgccggc catcatggcc acccgaacca tcgagaataa acgggaccgc
     1501 tatgcgacga tcctgattgc gccgttcatg acgtgctcgg cgcggctgcc ggtatacacg
     1561 ctcatcatcg cggccttcat ccccaatcac tctctgcttg gcccattgct gggcacgcag
     1621 gcggcggcga tgcttggcct gtacctactc ggatttctgg cggcattcgt tacggcgcgc
     1681 gtattgaagt cgtcggtgct caaaagcgag cgtaccccgt tcatgctgga aatgcctccc
     1741 tatcgcaagc cgacactgca aggactggcg cttcgtttac tcgatcgcgc caaagtgttc
     1801 ctgcggcgcg cgggcacggt gattttggcc gtcgcgattc tgctgtgggt gcttgcaaat
     1861 atcccgctac atgacggcag cgctccgccc attgagcaaa gcgtcgcggg aactgtgggc
     1921 cgggccgtgg agccgctgat ccggccgctg ggtttcgact ggaagatcgg tatcggcctc
     1981 atcacatcgc tcgccgcgcg cgaagtgatt gtcgggacgc tcggaaccat ctacggcatt
     2041 gaaggagaag ccgattcgaa aggccttcag caggcgctgc aacgcgacct ggatttgccg
     2101 ggcgccgtcg cactgctggt gttcttcgcg ttcgcgatgc agtgcatgtc cacgcttgct
     2161 gtagtgagac gcgaaaccgg cggctggaaa tggcctgtgc ttcagttcac ctatatgagc
     2221 gcgctggcgt atgtcagcgc attcgtcgcc aatcgtgccg tggcggcgtt tgtttagatc
     2281 ccaagtcact tctggaagaa caacattcgc gtagcaatca ccagcaggaa aattccgtat
     2341 aactttcgaa ccagcgccgg gggtagtccg aggagaattc tcgcgccgaa gtacgcgccc
     2401 aggaaaagcc ccaacgcgat gagcatggca tacttcaggt tcatatgccc ggcgcggtga
     2461 tactcgatgg cgccgagcag gctgacgggc ggtatcagcg ccgcaaggct tgtaccgatt
     2521 gcgccgagag gttccatctt catcaggatt acgagggccg gtacgataat cagcccgcct
     2581 ccgatgccga acatcccgga aaacacacct gctacgagcc cgatagccaa tgccgtgaag
     2641 acagtcatat gttcgaagag tctagcagat agccggtccc cacggagcgt cacctgtacg
     2701 ctgtatactc aggaatcaaa tgaaactgca tagttacacc atcacacagg ggcgggaccg
     2761 tgcaccggcc cgggcgatgc tgaaagccat cggattcacg gatgaagatc tccgaaaacc
     2821 ggttatcgga gtcgccaaca cctggatcga aaccatgccg tgcaactata accttcgcga
     2881 tctcgccgtg aaggtaaagg acggaatccg cgcggccggg ggcacgccca tggagttcaa
     2941 taccattgcg atcagcgacg gcgtcaccat gggtacggaa ggaatgaagt cgtcactcgt
     3001 cagccgcgac gtcatcgcgg attccatcga gttggtcgcg cgcggccaca tgttcgacgg
     3061 tatcgtcgcg ctggttgcct gcgacaagac catccccggc gcggccatgg cgctgttacg
     3121 gctgaacgtt cccggcctcg tcctctacgg cggctccatt ctgccggggc actacaatgg
     3181 ccgggacctg accattcagg atgtcttcga agccgtggga gccaatgcgt cgggccgtat
     3241 ttccgaccag gagttgctcg ccatcgagaa tgcggcctgt ccgggagcgg gagcgtgcgg
     3301 aggccagttc acggcgaaca ccatggccac gctcatggaa gttatcggcc tcagcccctt
     3361 gggaacggcg tcggttccac aggtggatcc acgcaagaac gacgtggcgg agcggtgcgg
     3421 ccgcgtcatc atggacgcgg tgaaactcga cctgaagccc agggacatcg ttacgcgcaa
     3481 agctttcgag aatgcgatcg cttcggttgc ggccaccggc ggatcgacga actccgtcct
     3541 gcacctctta gccatggccc gtgaagccgg cgtcgatttg aagatcgacg attttcagac
     3601 cgtcagcagc cggacgcctc tcctggtgga cttgaaacca gccgggcact ttgtcgcggt
     3661 cgatgtggac aaggcgggtg gcattcccgt catcgcccaa cggctggtcg aagggggcta
     3721 tgcggacggg tcggctatca ccatcacggg ccggacactg gaagccgagg cagccgaggc
     3781 gaaggaaaca ccgggccagg aagtgatcca tccactttcc aatccgatta agaaaagcgg
     3841 cggcctggtt atcctcaaag gctcgctggc tcccgaaggt tgcgtcatca aggtaaccgg
     3901 aatcgagcgc aaggggcaca cgggacc
//
LOCUS       DASTMK010000442        22267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_55218, whole genome shotgun sequence.
ACCESSION   DASTMK010000442 DASTMK010000000
VERSION     DASTMK010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 22267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22267
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55218"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFQ79_18865"
     CDS             complement(<1..1075)
                     /locus_tag="VFQ79_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU0141792.1"
                     /translation="MGFHLVVDYRPRGDQEAAIEQLVRGVLDRERHQVLLGVTGSGKT
                     FTMAKVIERIGKPALVLAHNKTLAAQLFHEFRSFFPSNAVEYFVSYYDYYQPEAYIPS
                     GDVYIEKEATINDELDKLRLSATKSLFERRDCVIVASVSCIYGLGSPEAYYGMLLMLE
                     KGQKIARQQILQRLVEILYERNDYDFRRGTFRVRGDVIEVYPTYDDYAYRIELWGDEI
                     ESLSQIDPVLGQVRQTYVRIPIYPKTHYVMTPEARDAAIHTIETELDWWRNELEKQGK
                     VIEAQRLYQRTMFDLEMIRQIGYCHGIENYSRHFSGRLPGEAPPTLLDYLPHDALVFI
                     DESHQTIPQLRGMYHGYRSRKETL"
     gene            complement(1121..1738)
                     /locus_tag="VFQ79_18870"
     CDS             complement(1121..1738)
                     /locus_tag="VFQ79_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141793.1"
                     /translation="MRVFYSVVVLWIVLSGNGWTQQREPAFEVYALTGAHYHGNLSLA
                     REWKPQYGAGVLAPLGRNWAAVFDVTTSATEAWWKHDGFPGTGPDDNFTRERRTIMVP
                     SVVRLWRRDRFSIYAGGGVAFEHRRQRTRQRPIVARGPNGEPILGPDFEELNPRSTNR
                     AAALRIGGIVSLTRKVVLRTGYSLLPRYADEKASQSLEFGIGYRF"
     gene            complement(1778..2671)
                     /locus_tag="VFQ79_18875"
     CDS             complement(1778..2671)
                     /locus_tag="VFQ79_18875"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0141794.1"
                     /translation="MRPIVALRRRIIQAGFWQRLWWVFRHSLVMTYRDGCLGTAKAIA
                     YSGLLAFFPILTVVATILVHANAEAVSRTIARLLFEIVPPGTEQLVERAFTVRGERPL
                     YLLIVAMLLSIWAAAGAMASLMEGFRAAYRIPTGRSAVKDRLVAILLVFFAALPVIGA
                     SALIVFGGRIQGRILTRIGVVSYGEQIQGWVSLLGTLINYAVALATVSLVAALLYHFG
                     PNRPKNWLAVWPGAMVATVLWLVVTSAFAWYVRNIADYNVLYGSIGAVIALLVWMYLL
                     ALVALIGCEFNAERERLSRIL"
     gene            complement(2673..4682)
                     /locus_tag="VFQ79_18880"
     CDS             complement(2673..4682)
                     /locus_tag="VFQ79_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141795.1"
                     /translation="MQTISRSRLLRFTESTAFCSVLLFVLNAWICWRLFFLEYPQYFS
                     SIEGAFISIARYASENWPHISWWPLWHCGMPYQDTYVPLLHLVVAAVAALSGFSAPHA
                     YHFVTAITYSLGPVTLFLLLRRLGAPSASAFIAALAYSLFSPSTMLMPDVRMDAGGLF
                     NARRLQVLTEYGEGPHITSIALLPLALLAIHRLLDRMDARRLALASLSIAAVLLTNTP
                     GSVSLVFAVFCLMAAQRVWSPRSLLRVMGAGFFAYGIAAYGLPWPTIKTNLANMERMH
                     AGFSYSLIRAGLVLLLLAALAAAGWGLGKLRTPLYVRFGSIYFLAMGALVLTGQIFAF
                     DIVPQPHRFQLEMEIGIVIVMAAAATAVFRRSSLPVRRLLFALGLAAAVFQISSYRAH
                     AKYKVRAVDPVSRSEYATAVWLEENMEGRRVFTSGSTAFWLNVFTDTPQLTGCCDQGM
                     PDRFQRPPVYLITATGSPHQTELSVLWMQAFGVHAVAVSGPESTDAYKDFRFPHKFEG
                     VLDELHRGAGDVIYRIPQASPMLSHVLRRDEIVWREPTDVEDVQEVRRYVNVLQDVSR
                     PMAAFNWMANNHAKIRAQMARDDLVSVQVTHSDGWQASANGKPARVLRDGLDFIVIEP
                     DCEGACEIDLVWTQSAGASASKWISAFSLLLAAGMLVLPRRRSPA"
     gene            4810..5952
                     /locus_tag="VFQ79_18885"
     CDS             4810..5952
                     /locus_tag="VFQ79_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141796.1"
                     /translation="MAEPANAFVDDVLGSNKAVLTAAAGLKRAIESSDPARVQFAVRD
                     LEQAASLREIDSETGGFRFATRPASADLSGTPAGPAPIPETLVAVLSDLQTANVLMAA
                     GTLTGEVEAPGTPQLNAAVADLEITTQSSTVRVQGAAGFGFAGETLPEPSPDVPSALT
                     QFQARCEEALKMLVEQVRGVLNSIIEKLSGLDKDKVLDALNRIGQSAGLPNFGRLYQK
                     AIDIIGRALETLRSLLGEGAVTFVREKATAFWNGLTSGKLIEDLLAHLFDVSGVKQRI
                     AAIAARQDLEIAPLDTASGDLAGLEEKLKRTMSTLGAIATAIGTAAALLALAPISGQT
                     LAVITAGAYVLVLAAAVVIGMDYADSEKVVQRVRGIGGIVSSVAKP"
     gene            5936..7102
                     /locus_tag="VFQ79_18890"
     CDS             5936..7102
                     /locus_tag="VFQ79_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141797.1"
                     /translation="MSPSLSSNDFAGIRIGELIAARITALDSELQRTVWLLNERIAEP
                     LRTRVLARLSRVLGEARQDLNRLAKDLPNLKADESARRYRAIREQCDRLFRECLAVIE
                     GSLYREAGADGGLCRIADSLIDDLSRRSDEPWGRFTILGEGEYFGEMAEIIRLRFPEN
                     SIWSLPLAAHEFGHFFARSAEVKRNDGSDLDSLRTRLMLAKKHSDYAWRRVHEHFADL
                     FATYAIGPAFACVSILLRFDPESAWQAFAEHPPDADRAEMILMGLRKKQEYRWIADFL
                     EATWRDALAQCGQKTVHEQTDLPAFKALWDELDFVFSHPLEGIAYDGWLRAVPLQAEL
                     SDLNQSRIVPPAGSNIADVLNAAWSGRLKLHPGATATIDLLGQRAFVLCDRLAA"
     gene            7118..8482
                     /locus_tag="VFQ79_18895"
     CDS             7118..8482
                     /locus_tag="VFQ79_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141798.1"
                     /translation="MKDDILEARLIFLDEQLERAENALRWLEGSQILQNLGYDMQKIA
                     APLETTIVKEARLQRKSIERAQAELNLAAAGSTGEPHLAAAWQMYNQSLEKSQELLGQ
                     WLELIGGIALRDKQFDCNVCDLADELIRTCCDDTIGPTIKAPDLFVTVPAQQDAVSKT
                     WFRMIRLRFPHWTVWHLPLTASEFGYVLFDEWDDLSEFLDEQVKLWGRPGDKQKPAPK
                     TGPRRRNIKSHLRKLIADGFAALTMGPAYAYSAFYLNFSAGRAETEDLPSDAARAFVV
                     LRMIDLMNQHESEKPYREHIERLDEKWKASCKFAEDQERQTELEEGKLNSLCRAVWNF
                     LNDQFRFTGRYPYLNGDQGWNTAQRIRRHLENPQQYPMSFTSSTRLRDVLNAGWLRRI
                     EIPAGAEHDFQVKQLAKAIMALCRHIVDQRRGIPARAEQPQQGSIQIPTLGGGAEVKT
                     VGGR"
     gene            8513..11587
                     /locus_tag="VFQ79_18900"
     CDS             8513..11587
                     /locus_tag="VFQ79_18900"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0141799.1"
                     /translation="MEKSPISDLLANLPGSGNAVTDPFAAARTIRESIQRARDALRAK
                     DYGQVRNMLGALADRPVQVRHYPNMMSTSQAPSVDASCVVHLIAIARAIFGQASLAQQ
                     NSQEAANLFETAVAELPVTPEELDLSGEDYADLAVALEAAGRRQVAISYWRQAVSRGL
                     QYAVSLLKAGTTLMEAGLVGEAQEAFRKSVHIHPSAAAFEKLAEATELQNDAAAAAPM
                     YTSAAQIALSDGDAAHALELVNKALESGAGKVSALGVKAQVLHNLGRYEEAVQAAGEA
                     LALDPDSIPALLWKSFALGELKDWENALQACQHILSLQPGMLAASLNMARVVYELGRK
                     EEAAQIVEDAVKDTPGVPDGAYAGLAAIYVELNEADKAIAAAGKALEINPNNLQALTS
                     KTMAMLSQERLEESLPQLDRMAELATENRDTILYTRGIVLEGLERLEESIASLRAARE
                     YNPSRTDVLERLAKLLARTGDTPEAIEIIDQLLVLEPERTDILAEKARLLILVDKCEE
                     ARQYADRALARDPGNYLALFVRGLALFGLSLYEEALAAFDACMSLDDGKLAAAWKGKA
                     LNKLNRHAEAAEVLGKLVEQGDEDTSVLRALSESLIYSGKPDDAAQLLRQLVAQHPDA
                     PEYVELLSQAFEKSGRINDALQHLDTYLAGNPRDTLILGCAAKLLRQAGRAAESATYY
                     ERLADVEPENLSHLQNLATALLSAGSNEKALEAVERALRLEPESQQSRAYKAEILRVL
                     NRFEECFAACDALLAENPNNDYVLATKGTALRFLDRTEEALECLNAAVIQNPANDFAR
                     LNKAHALCDAAEFNVALDELDQVSQQERETGTYYWILLGWALENLGRDRTEEALRAYD
                     YCVSRDPEDIYSRRGRADMYRRLGKTDRAAEDYEWLIRRADSQAGTAVPDLLAMKGWS
                     MYGLGRPEDGVKFLVDALSLDPSLISSQFDLGLIFLCCGRAAVALREYQRGIDLTNGK
                     TPARRKGLLSVAVRDLRDALADRPELERVRQVRQIREMLQSELRPRESAAAQ"
     gene            complement(11603..12214)
                     /locus_tag="VFQ79_18905"
     CDS             complement(11603..12214)
                     /locus_tag="VFQ79_18905"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU0141800.1"
                     /translation="MSFSLPALPYPPDALEPHIDKMTMEIHHGKHHGAYVNNLNKALE
                     SAPDLAGKSIEELLANNLAIVPENIRTAVRNNGGGHANHSMFWTIMAPKSGGKPTGEL
                     SDAISATFGSFDSFKEKFNAAAMSRFGSGWAWLVKDGNKVDILSTANQDSPIMEGKLP
                     VMGLDVWEHAYYLKYQNRRAEYVEAWWNVLNWKEIEKRFTSGK"
     gene            complement(12272..13711)
                     /gene="gatA"
                     /locus_tag="VFQ79_18910"
     CDS             complement(12272..13711)
                     /gene="gatA"
                     /locus_tag="VFQ79_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU0141801.1"
                     /translation="MNIATLSIDQVREGLKERAFSAVELTREALEFAQAENPKTNAYL
                     HFSTERALEAAARVDEGIARGENIGPLGGVPIAVKDVIVTKGVRTTCGSKLLAEYVPP
                     YDATAVGRLEQAGGILIGKTNCDEFAMGSSNENSAFGPVRNPVALDRVPGGSSGGSAA
                     AVAQGTAVVALGSDTGGSIRQPASFCGLVGVTPTYGRVSRYGLTAFASSLDHVGPFAR
                     NVKDAATLLRVLAGRDPLDSTSAFAPVPDYPVDMDGGVEGMKIGLPREYFRDLSSETG
                     DLILQGVETLKKLGCEVRDISLPHTAYAVGCYYIICTAEASSNLARYDGVRYTARTSG
                     DTLIDMYRNTRGAGFGLECKRRIMLGTYVLSAGYYDAYYLKAQKVRTLITRDFADAFG
                     SVDAIIAPVSPSPAFRLGEKTDDPMEMYLSDIYTLTGSLAGIPCMSVPCGKTVEGLPV
                     GMQLMTRHFGEADMFRLAGAYEKASSFFA"
     gene            complement(13708..14007)
                     /gene="gatC"
                     /locus_tag="VFQ79_18915"
     CDS             complement(13708..14007)
                     /gene="gatC"
                     /locus_tag="VFQ79_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU0141802.1"
                     /translation="MKISEQEVRYVAELANLRLTDEELGRMAAELDKILTHMDKLNEL
                     DTSAVEPMAQVLYEAGETATLRDDVERTPLGEEAALANAPLSGAGHFKVPRVIER"
     gene            complement(14004..14366)
                     /locus_tag="VFQ79_18920"
     CDS             complement(14004..14366)
                     /locus_tag="VFQ79_18920"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEU0141803.1"
                     /translation="MKNVSPEAVATRAWSIAVGKIIAAHSNVAAVVDKKLVIDVEDFE
                     WQRQLFTLRRQILARMEQVAGPGLFTDLQFRIAVPRRMPQREERVSGVAPVRDEADGI
                     RDPSLRHAYKMARKRASA"
     gene            14440..15003
                     /locus_tag="VFQ79_18925"
     CDS             14440..15003
                     /locus_tag="VFQ79_18925"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU0141804.1"
                     /translation="MNLILASASPRRSELLASAGIRFTVRVSQVEERHVPGEDAWQYV
                     KRLAEAKARTIESTPGEVVLGADTVVVVDDHILEKPADHADAARMLRLLSGRRHQVIT
                     GICLRGLNRITVDAETTEVRFVSLSEAEIDDYVRTGEPMDKAGGYAIQGLASKFIDRV
                     EGCYFNVVGLPVALVYQKLKEFEGMHE"
     gene            14996..15649
                     /locus_tag="VFQ79_18930"
     CDS             14996..15649
                     /locus_tag="VFQ79_18930"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU0141805.1"
                     /translation="MSKRATVGVTYRLEEKLKPYEDALRAAGLDVVRIQAGSSSKLDG
                     LGGLVLTGGTDIDPSFYGEKPAPETQTPDRERDEMELSLLQQALSVDLPVLAICRGMQ
                     LFNVAHGGALIQHLGEGSPHTRGRDVDHVHSIITVEGTHLSRILGPGEQVVNSRHHQA
                     VDPDRTGKGLIVSAKSPDGVIEGLEMPGQRFAIAVQWHPEDLFRLDARLFQAFAEAV"
     gene            complement(15959..17233)
                     /locus_tag="VFQ79_18935"
     CDS             complement(15959..17233)
                     /locus_tag="VFQ79_18935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0141806.1"
                     /translation="MSDELSRRNFIRNSAGVALAAGPAILPALGANDKVQVGWVGTGS
                     RGNHVMGMMYKAVPELVTVTAVCDTYTGNLNRAKDAVQTQGGNTPKTYVDYREVLRDS
                     SIDVVFICTPEHLHHEMAVAALNAGKHIYLEKPLAHTIEEGWDIVNAAKKAGKVVQVG
                     TQNRSNSLYIKAKEMVEQGMIGDVHYVRAFWYRNSLDDNPAWRYRIPPDADPQNTDWK
                     RFLGPASNRAFDKQRYYQWRLYWDYSGGISTDLLVHQTDITNFVCGKTVPSSCMASGG
                     IYRWTEDDREVPDCLSAIYEYPDRFHINYSCYFGNDHFGYGEQFMGNEGTIEVNSRQF
                     LSFWPEKFGGKPPEKVAARQEIRIEAKGNDANAVQAHVKNFIMAVLGKEKAIAPAEIG
                     QQAAIGGHMATLSFKRGKKILWDDKTNKYRFV"
     gene            17350..20556
                     /locus_tag="VFQ79_18940"
     CDS             17350..20556
                     /locus_tag="VFQ79_18940"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0141807.1"
                     /translation="MAVSLLLLLAGNLFAGRLERYALILEGNPAARQASSSKPGQPRF
                     AEDQRQKLVAAQSNVRRALADRKIRVTGAVQTIANAVFVVASEEQAAALRTLPGVAGV
                     QLMRPVKRSLNRAIDLMNVSGAWSALQGEGNAGAGMRIAILDTGIDQTHPAFQDGSLQ
                     IPAGFPKCAGQGCEYTNNKVIAARSYVQMLVLPEDPADSRPDDLSPRDRVGHGTAAAS
                     VAAGVRNGGPAATITGVAPKAFLGNYKIFGSPGVNDVTFTDVVIQALEDAYNDGMDVA
                     SLSLGFAAVWGPLDSGSVCGNSPGVPCDLFAAAVEEAAQAMVVVVSAGNDGDLGNLAP
                     SLNTIHSPGTAPSAITVGAIYNSHEFFSSVRASGLDRINALFGNGPKPGGPISAPLRV
                     VSRSEDQKACTSLQSGSLAGAIALIERGDCGFDTKVINAQRAGAVGVILHQSQGSNFV
                     FPPTGLSETGIPAVLIGYDAATRLSSSAASNADFQVTLDPALVEVNATSNQVAYFSSY
                     GPSIATFGIKPEISATGTDLYMATQKYDPNGDLYDATGYTNAQGTSFAAPIVAGAAAL
                     VMQAHPNFSVADVKSAIVNTTSADVSDIDGEGRVVPARVAAVGAGKLDVSAAVQTQIT
                     SDVATISFGAIGAGSSGVSRSFRLTNSGTDSVQLSVRVAERDTDAGGRVSIVGETSFT
                     LAPGTRTTNINARLDLTQRNPGSYEGFVNVMQGSRAVLRIPYVYYVPGSGPFNIIPLT
                     GFDFTGIADQRLPGRLTLKVIDRFGIPVPDLPVQFRPANAIDTATETTDSLGIAEAQV
                     FLGPQLGEQEFSADVGGSTVYFPGKAIQRPAIFTNGVVNAASNQVGQGLAPGSLISIY
                     GSALSQAIRGVFTPYLPVSLAGMSVSFDRPSDNVSVPGRLHYVSPDQINLQIPWELEG
                     YNNAIMKISIGNFSSGLYDVPLARYSPAFYEYNDAGSGRTLIAALDEGNVLVTPSRPA
                     RKNTVVQFYANGLGPVDNRPASGELSPTSPLARTRVIPEVRIGGRPAHVDFSGLAPTF
                     AGLYQINAVIPGDAPSGLQPVEITMEGVTSKTAFLPVE"
     gene            20572..>22267
                     /locus_tag="VFQ79_18945"
     CDS             20572..>22267
                     /locus_tag="VFQ79_18945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0141808.1"
                     /translation="MNRIWIAVLALPLAAQIVPNRYIVELSGEPAATYAVKTKGNLRA
                     ALADRRAAIQSEQLPVRQAVQNTGGTVLASVDTVANALVVEIPDSQAASLNRLPGVLR
                     VHKVWEGKLFLDHALPLSRVPEAWMEAGGEDRAGAGMKIGIIDTGLDADHPAFFDSNL
                     QPAGVPAAGFENNPRIMVARSYDRGPVLSGQGARDLNGHGTGVAMVTAGGRTAAPLAT
                     ISGVAPKAYLGIYRVFSGNSGSFSSDLLLRALDDAVADGMDVVNLSLGLTAPLRPSVD
                     PFYNAVERAAAAGVIVVAAAGNEGPYPGSVGPPASAPSTIAVGATANSRIFAAFVQPD
                     GDTPITAEPGSRSATGAAVTGPMIDLAAIDSSALSCSALPADALSGHIVLILRGECTF
                     ETKLNNAQNAGALGAVVYTQAEDPGLFRMETGSVALPALSVSHEDGLRLKALLQEQPE
                     LTATLQLSRAAVPADPNNLADFSSTGPNTDAGIKPDLVATGTNVYTAAEKTDPSGESY
                     DASGFLVQSGTSFSAPLVSGAAAVLKGARPGLTAAQYRSLLINSTARFLFTGGQAAS"
BASE COUNT         4618 a         6641 c         6720 g         4288 t
ORIGIN      
        1 ccagcgtctc ttttctcgaa cggtagccgt gatacatgcc tcgcaattgc ggaatggttt
       61 gatgactctc gtcgatgaac accagagcgt cgtgcggcag gtagtcgagc aaagtaggcg
      121 gggcttcgcc gggcagacgt cccgaaaaat gacgggagta gttctcgatc ccatggcagt
      181 agccaatctg ccggatcatc tccaggtcga acatcgtcct ctggtacagg cgctgggcct
      241 caatcacctt gccctgtttt tccagttcat tccgccacca gtccagctcc gtttcaatgg
      301 tgtggatggc ggcgtcccgg gcctccgggg tcatgacgta gtgagtcttc ggataaattg
      361 gaatccgcac gtaggtttgg cgaacctggc ccagtacggg atcgatctgg gacagagatt
      421 cgatttcatc cccccataac tcgatgcgat aggcgtaatc gtcgtaggtg ggatagacct
      481 cgatcacatc gccgcgcacc cggaacgttc cgcgccggaa gtcataatcg ttgcgctcat
      541 acaggatctc aaccagccgc tgcaaaatct gctggcgcgc gattttctgc cccttttcga
      601 gcatcagcag catgccataa taggcctccg gcgaaccgag gccgtatata cagctcacgc
      661 tcgcgacaat gacgcagtcg cggcgctcaa acagggactt ggtagccgag agccgcagct
      721 tatccagctc gtcgttaatg gtggcttcct tttcgatata cacatcgccg gaagggatgt
      781 aggcttccgg ctgatagtaa tcgtagtagc tgacgaagta ctcgacggcg ttggatggaa
      841 agaaggaacg gaactcatga aagagctgcg ccgccagcgt cttgttgtgg gcgagaacca
      901 gcgccggctt gccgatgcgc tcgatgacct tcgccatggt gaaggtcttt ccggacccgg
      961 tgacgccgag gagaacctgg tgccgctcgc ggtcgagaac gccccttaca agctgttcga
     1021 ttgcggcttc ctgatctccc cggggacgat aatcgacgac gagatggaag cccatgctat
     1081 ccactctagc gcagcgccgg aagacggcac cggcctcgtc tcaaaagcgg tagcctatgc
     1141 caaattccaa gctttgcgat gccttttcat cggcgtagcg tggaagaagg gagtaaccgg
     1201 tccgcagcac aactttgcgg gtcaggctta cgatgccgcc tatacgtaaa gccgccgccc
     1261 tgtttgtgct cctgggattc agctcctcga aatcaggccc cagaatcggt tcgccattgg
     1321 gaccccgggc gacgatcgga cgctggcggg tgcgctgcct cctgtgctcg aaggcgacac
     1381 cgcctccggc atagatggag aagcgatccc gcctccagag ccggaccacg gaaggcacca
     1441 tgatagtgcg ccgctctctt gtaaagttgt cgtcaggacc ggtgccggga aagccgtcat
     1501 gcttccacca ggcctccgtc gcgctcgtgg tcacatcgaa gacagccgcc caattcctgc
     1561 ccaacggagc cagcacgcct gcgccgtatt gtggtttcca ttcgcgcgca agcgatagat
     1621 tcccgtggta gtgcgcgccg gtgagggcgt agacttcaaa ggccggttcg cgctgttgcg
     1681 tccaaccgtt gccggagagc acgatccata ggacaacaac cgaataaaag acccgcatag
     1741 cattattcta tgcgtgcagc gcgggtgtcc gcccgcctca caggatccgg ctcaggcgct
     1801 cgcgttccgc attgaactca cagccgatca atgcaaccag agccaacaga tacatccaga
     1861 caagaagagc gatcactgct ccgatactgc catagagcac gttgtagtct gcgatgttgc
     1921 gtacatacca tgcgaacgcc gatgtcacaa ccagccacag aacggtagcc accatagcgc
     1981 cgggccagac ggccagccag ttttttggcc gattggggcc gaagtggtag agcagtgcgg
     2041 ccacaagact gacagttgcc aacgccactg cgtagttgat cagggttccc agcagcgaca
     2101 cccagccctg gatctgttcg ccataggaaa ccaccccgat tctggtgaga atgcgtccct
     2161 gtatccggcc gccgaacacg atcagggcgg aagcgccgat gacggggagg gcggcgaaaa
     2221 acacgagcag aatcgccacc agccggtcct tcaccgcgga gcgcccggtc ggaatacgat
     2281 aggccgcgcg aaagccttcc atgagactgg ccattgcccc ggccgcggcc cagatggata
     2341 gcagcattgc gacaatcaac agatacagag gccgctcacc ccgcactgta aacgcccgtt
     2401 cgacaagctg ttctgttccg ggcggcacaa tctcaaacag aaggcgcgcg atggtgcggg
     2461 acacggcttc cgcattcgca tgcaccagga tggtggcgac aaccgtcagt atcggaaaga
     2521 aagcgagcag ccccgaataa gctatcgcct tggccgtacc caggcatccg tcgcggtagg
     2581 tcataaccag cgagtgccgg aacacccacc acaggcgctg ccagaatccg gcttggatga
     2641 ttcttcggcg cagtgcaacg atgggacgca tcttaagcgg gactccgtct gcgcggaagg
     2701 accagcatgc ctgccgccag caacaacgag aatgccgaaa tccacttcga ggcggatgca
     2761 ccggcgctct gggtccagac gagatcgatc tcgcaagcgc cctcacagtc cggctctatc
     2821 actataaaat ccagcccgtc acgcaacacg cgggcaggct tgccgttggc cgacgcctgc
     2881 catccatccg agtgtgtcac ctgaaccgac acaagatcgt cgcgcgccat ctgcgcgcgt
     2941 attttggcgt ggttgtttgc catccagttg aacgccgcca taggccggga cacatcctgc
     3001 aacacattta catagcggcg cacctcctgc acatcttcca catcggttgg ctcgcgccat
     3061 acgatttcgt ctcttcgaag tacgtgagaa agcatgggag aagcttgcgg aatccggtaa
     3121 atcacatccc cggcgccgcg gtgaagctcg tcgagcacac cttcgaactt gtgtggaaaa
     3181 cggaagtctt tatacgcatc cgtgctctcc ggaccgctga cggcaacggc gtgaacacca
     3241 aaagcctgca tccataacac ggagagttcg gtttggtgcg ggctcccagt tgcagtaatc
     3301 aggtaaaccg ggggccgctg gaaccggtcc ggcatgccct gatcgcagca accggtcaac
     3361 tgcggcgtat cggtgaagac gttcaaccag aacgcggtgg acccgctggt aaagacgcgg
     3421 cgcccttcca tgttctcctc gagccagacg gcggtggcgt attcggagcg agagacaggg
     3481 tcgacggctc gaaccttgta cttggcgtga gcccggtagc tggaaatctg aaagacggcg
     3541 gcggcaagac ccagtgcaaa caacagccgg cgtaccggaa gtgaagaccg ccggaaaact
     3601 gctgttgccg ccgcagccat cacaatcacg attccaattt ccatctccag ttgaaagcgg
     3661 tgtggctgcg gcacgatatc gaatgcgaag atctggccgg tcaggacaag agcccccatc
     3721 gcgagaaagt agatagagcc gaaacggacg tatagcggcg tgcggagttt gcccaggccc
     3781 caaccggctg cggcaagagc tgccagtaat agaagaacga ggcccgcccg tatcaggctg
     3841 taggagaacc ccgcgtgcat cctctccata ttcgcaaggt ttgttttgat ggtcggccag
     3901 gggagcccat acgcagcgat gccatacgcg aaaaaacccg cgcccatcac acgaagcagg
     3961 ctccggggac tccagacccg ttgtgccgcc atcaggcaga agaccgcaaa tacaagcgag
     4021 acgctgcccg gcgtattcgt caacagcacg gcggcaatgg agagcgaagc cagggccagc
     4081 cgtcgggcat ccatgcggtc cagcagcctg tgaattgcga ggagagcgag cggcagcaaa
     4141 gcaatcgagg taatgtgcgg cccttcgcca tattccgtga gcacctgaag tctcctcgca
     4201 ttgaaaagac ctcccgcgtc catacgcacg tccggcatga gcatcgtgga cggcgaaaac
     4261 agcgagtacg ccagcgccgc gataaacgcc gaagccgaag gtgcgccaag cctgcgcagc
     4321 agcagaaaca acgtgacggg accaagcgaa tacgtaatgg ccgtaacgaa atggtatgca
     4381 tgcggcgcgg aaaaaccgga aagagccgcg acggcagcca cgacgaggtg caacagcggt
     4441 acgtaagtat cctggtatgg catcccgcag tgccatagag gccaccacga aatatgcggc
     4501 cagttttccg aagcgtagcg ggcaatcgag atgaatgcgc cctcgatgga gctgaagtac
     4561 tgcggatact cgagaaaaaa gaggcgccag cagatccagg cgttcagcac aaacagcaac
     4621 acggaacaaa aggcagtgga ttcggtgaag cgcagcaacc gggaacggga aatagtttgc
     4681 actgggaacg taaccatcca tggtagcgcc agggcactcc tacaatgagt agcaaaatca
     4741 gtaccgtact tgtatccgta atggtccgcg cgccgtatac tgtaagcacc tgactggagg
     4801 tacaaagaca tggcagaacc tgcgaacgca ttcgtggatg acgtgttggg gagcaacaag
     4861 gccgttctga cggcggccgc gggattgaag cgagcgatcg aatccagcga tcccgcccgg
     4921 gtccagtttg ccgttcggga cttggagcag gcagccagcc tgcgggaaat agatagtgag
     4981 acgggcggct ttcgtttcgc aacgcggccc gcttcagccg atctgtccgg tactcccgct
     5041 ggaccggcac ccataccgga gaccctggtc gcagtcttga gcgacttgca gacggcgaat
     5101 gtcctgatgg ccgcggggac gctgaccggg gaagtggagg ctcccggcac tccccaactg
     5161 aatgcggcgg tggcagacct ggagatcaca acacagagtt cgacggtacg ggtacaagga
     5221 gccgctggat tcgggtttgc cggagaaacc ctgccggagc cttcccccga tgttccttcc
     5281 gcgctcacgc agtttcaggc ccgctgtgag gaagccttga agatgctggt ggaacaggtc
     5341 cgcggcgtat tgaacagcat tatcgaaaag ctttcgggcc tggataaaga caaggtgctg
     5401 gatgcgctga atcgtattgg ccaatccgcg ggtctgccaa acttcggacg gctgtatcaa
     5461 aaagcgatcg atatcatcgg gcgtgctttg gagacgcttc gctccctgct cggcgaagga
     5521 gcggttactt tcgtcaggga gaaggcgacg gcgttttgga acggtctgac aagcggaaaa
     5581 ctcatcgagg atctgctggc acatctgttc gacgtgagcg gcgttaagca aagaattgcc
     5641 gccatcgcgg cgcggcagga tctcgagatt gctcccctgg acacggcgag cggcgacctg
     5701 gccggcctcg aagagaagct caaacggacc atgtccacgc tcggggcgat cgctaccgcg
     5761 atcggcacag ccgctgctct tctggcactc gcgccgatct ccggacagac gcttgcggtc
     5821 atcaccgccg gagcgtacgt gctggtactg gcggcggcgg ttgtgatcgg catggattac
     5881 gcggactcgg aaaaggtggt gcagcgcgtc cgtggcattg gtgggatcgt ttccagtgtc
     5941 gccaagcctt agctcgaacg attttgccgg cattcggatc ggagaactga tcgccgcgcg
     6001 gatcactgcg ctcgattccg agttgcagcg gaccgtgtgg ctgctcaacg aacgcatcgc
     6061 ggagccgttg cgcacgcgcg tcctggcgcg gctgagccgc gtgctcggag aggcacggca
     6121 ggacctgaac cggctcgcaa aggatctgcc caatctgaag gctgacgagt ccgcccggcg
     6181 ataccgcgcg atccgggaac aatgcgaccg gctgttccgg gagtgccttg cggtcatcga
     6241 gggctcttta tatcgcgaag cgggcgccga cggcgggttg tgcaggatag ccgattccct
     6301 catcgacgat ctgagccggc gctcggatga gccgtggggg cgcttcacca tcctgggcga
     6361 aggcgaatac ttcggcgaaa tggcggagat catccggctg cggtttcccg agaattccat
     6421 ctggagtctg cccttggccg cgcatgaatt cgggcacttc ttcgcacgga gcgcggaagt
     6481 gaaacgcaac gatggtagcg atctcgacag cttgcggacg cgcctcatgc tggcgaagaa
     6541 gcattcggat tacgcctggc ggcgtgtgca cgaacacttt gcggatctct tcgcgacgta
     6601 cgccatcggg ccggcgttcg cctgcgtatc gattttgtta cggttcgatc cggaatcggc
     6661 gtggcaggcc tttgcggaac acccaccgga tgcggaccgg gcggagatga ttcttatggg
     6721 acttcgcaaa aagcaggaat accggtggat agcggatttt ctggaagcga cgtggcgcga
     6781 tgcgctggcg cagtgtggcc aaaagacggt tcacgagcag acggacctgc cggcgttcaa
     6841 ggcgctgtgg gacgaactcg atttcgtgtt ttctcatcca ctcgaaggaa tcgcgtatga
     6901 tggctggctg cgcgctgtcc cgcttcaggc cgaactgtcg gatttgaatc aaagccggat
     6961 cgttccgccc gccggatcga acattgccga cgtattgaat gccgcctggt ccgggcggct
     7021 caagcttcat cccggcgcaa cagctacaat tgatctcctg gggcagagag catttgtctt
     7081 gtgcgaccgg ttggcggcgt gagccgcggg agggccaatg aaagacgaca ttctggaagc
     7141 gaggctgatt tttctcgacg agcagttgga gcgcgcagag aacgctctcc gctggctcga
     7201 gggaagtcag attctgcaaa acctcggcta tgacatgcag aagattgcgg cgccgctcga
     7261 gaccacgatt gttaaagagg cccggttgca gcgaaagagc attgaaaggg cacaggcgga
     7321 actgaatctg gctgccgcag gctctaccgg cgagccgcac ctcgcggctg cctggcagat
     7381 gtacaaccag tcgctcgaga agagccagga actgctgggg caatggctgg agctcatcgg
     7441 cggcatcgct cttcgcgaca agcaattcga ctgcaacgta tgcgaccttg ccgacgaact
     7501 catccgcacc tgctgcgacg acacaatagg gcccaccatc aaagcacctg acctctttgt
     7561 tacagtgccg gcgcaacagg acgcggtcag taagacctgg ttccgcatga tccggttgcg
     7621 gttcccgcac tggaccgtct ggcacctgcc gctcaccgcg tcggaattcg gttacgtttt
     7681 gttcgacgaa tgggacgact taagcgagtt cctcgacgaa caggtgaaac tatggggcag
     7741 gcccggcgat aaacaaaagc cggcgccgaa gaccggcccg agacgccgca atatcaagtc
     7801 gcacctgcga aagctgatcg ccgacggatt cgcggcgcta acgatggggc cggcttatgc
     7861 ttactcggcg ttctatctga atttcagtgc gggccgcgcg gaaaccgagg atttgccttc
     7921 ggatgccgct cgcgctttcg tcgtgctgcg catgatcgat ctcatgaacc agcatgagag
     7981 cgaaaagcct tacagggagc acatcgagcg tctcgacgaa aagtggaaag caagttgtaa
     8041 gtttgcggaa gaccaggagc ggcagacgga attggaagaa ggaaagctca acagcctgtg
     8101 ccgggcggtg tggaattttc tgaacgacca gttccggttc accggccgct atccctatct
     8161 caacggcgac cagggttgga acaccgccca gcgtatcaga cgacatctcg agaatccgca
     8221 gcagtatccg atgtcattta ccagttcgac ccggcttcgc gatgtgctga acgcgggctg
     8281 gctgcggcgc atcgagattc cagccggggc cgagcacgat ttccaggtga agcagctcgc
     8341 gaaggcgatt atggcccttt gccgtcatat tgtggaccag cgcagaggca ttccggcacg
     8401 tgcagaacaa ccgcagcaag gcagtatcca gatcccgacc ctcggcggcg gagccgaagt
     8461 gaagacagtc ggagggcgct gagggttatc ggccgaaagg gagcctgacg tcatggagaa
     8521 atcaccaatc agcgatctac ttgcgaacct gccagggtct ggcaatgcgg tcacggatcc
     8581 gttcgcggcg gcacgcacga tccgggaaag catccaaagg gcgcgggatg cgctcagggc
     8641 gaaggactac ggacaggtgc ggaacatgtt gggcgccttg gcggaccggc cggtacaggt
     8701 cagacattat ccgaatatga tgtccacctc acaggcaccg tcagtcgatg cttcctgtgt
     8761 ggtgcattta atcgcgatcg cgcgcgcgat ttttgggcag gcctccctgg cgcagcagaa
     8821 tagccaggaa gccgcaaatc tattcgaaac ggctgtcgcc gagctgcccg tcacacccga
     8881 ggaactggat ctatcgggcg aggattatgc cgacctcgcg gtggcattag aagccgccgg
     8941 ccgcaggcaa gttgccatca gttactggcg ccaggcagtg tcgcgagggc ttcagtatgc
     9001 ggtcagtctg ctcaaagcag gcaccacatt gatggaggcc ggtctcgtgg gggaggcaca
     9061 ggaagcgttc cggaaatccg ttcacatcca cccttccgcg gctgccttcg aaaagctcgc
     9121 cgaagcaacg gagttgcaga atgacgccgc cgcagccgct ccgatgtata ccagcgctgc
     9181 ccagattgcg ctctcggatg gcgatgccgc tcacgctctg gagctcgtca ataaggctct
     9241 cgaatcggga gccgggaaag tatccgccct cggggtgaaa gcacaggtct tgcacaacct
     9301 gggccgctat gaagaggccg tgcaggcggc cggcgaggcg ctcgcactcg atcccgattc
     9361 catcccggcc ctgctctgga aatccttcgc gctgggcgag ctgaaggatt gggaaaacgc
     9421 tctccaagcc tgccagcata ttctcagcct gcaacccggc atgctcgcgg cttccctcaa
     9481 catggcccgc gtggtctacg agctcggccg gaaagaagaa gcggcacaga tcgtggagga
     9541 cgcggttaaa gatacgccgg gtgtgcccga tggcgcctat gcgggtctcg cggccatcta
     9601 cgtggagctg aacgaggccg ataaagcgat cgccgcagcc ggcaaagctc tcgaaatcaa
     9661 tccgaacaac ttgcaggccc tgacgtccaa aaccatggcg atgctcagcc aggagcggtt
     9721 ggaggaaagc ctgccgcagc tcgatcgcat ggcggagctc gcaaccgaaa accgcgacac
     9781 catcctctat acgcgcggaa ttgtcctgga gggtctcgaa cgcctggagg aatccatcgc
     9841 atcgctgcga gcggcaaggg aatataaccc atcgcgcacg gatgtgctgg agcgccttgc
     9901 gaagctgctc gcgcggacag gagatacccc ggaagccatc gaaatcattg accagttgtt
     9961 ggtgctggag cccgaacgga cggacatcct cgctgagaaa gcgcgcctgc tgattctggt
    10021 cgacaagtgc gaagaggctc gccagtacgc cgaccgcgcg ctcgcgcggg accccgggaa
    10081 ctatctcgca cttttcgtca ggggactcgc gttattcggt ctctcgctct acgaagaggc
    10141 gctggcggcc ttcgatgcct gtatgtcttt ggacgacggc aaactggctg ccgcctggaa
    10201 aggcaaagcg ctcaacaaac tgaatcgtca cgcggaagcg gctgaagtgc ttggcaagct
    10261 cgttgagcag ggtgacgagg atacttccgt gttgcgcgct ctgtctgaga gcctgattta
    10321 ttccggcaaa ccggacgatg cggcacagtt gttgcggcaa ttggtggcgc agcaccccga
    10381 cgctcccgag tatgttgagc ttctttccca ggctttcgag aagtcaggcc gtatcaacga
    10441 tgcattgcaa catctggaca cgtatctggc gggaaatccc cgcgatacgt tgattcttgg
    10501 ttgcgcggcc aagctgctgc gccaggcggg ccgtgcggcg gaatccgcaa cttactatga
    10561 gcgtctcgct gacgtggagc cggagaacct gtctcatctg caaaacctcg ccaccgctct
    10621 gctctccgcc ggcagtaacg agaaggcgct ggaagcggtg gaaagagctt tgcgtctgga
    10681 accggaaagc cagcagtcgc gcgcctacaa agccgagatt ctgcgcgttt tgaaccgatt
    10741 cgaggaatgt ttcgccgcct gtgacgctct tttggcggag aatccgaaca acgattacgt
    10801 cctggctacg aaaggcaccg cgcttcgctt cctggaccgc acggaagagg ccctcgagtg
    10861 cctgaacgca gcagtaattc aaaatcccgc aaacgatttc gcccgtttga acaaggcgca
    10921 tgctctttgc gatgccgcgg agttcaacgt cgcgctggac gaactcgacc aggtcagcca
    10981 gcaggaacgc gagacaggca cttactattg gattctgctc ggctgggcac tcgaaaacct
    11041 ggggcgagac cgcacggaag aagccctgag agcctatgac tattgcgtgt cgcgggatcc
    11101 ggaagacatc tatagccggc gcggaagagc ggacatgtac cgccggctgg gcaaaacgga
    11161 tcgggctgcc gaagactatg agtggctgat ccggcgggcg gattcccagg cgggaactgc
    11221 cgttccagac ctgttagcca tgaaaggatg gagtatgtac ggcctggggc gtcccgaaga
    11281 tggcgtgaag tttcttgtgg acgcgctttc gctcgacccc tcgctgatca gctcccaatt
    11341 cgacctgggc ttgattttcc tctgttgcgg ccgcgcggcg gtagcgctgc gggaatacca
    11401 gagaggcatc gatcttacta acggcaagac gccggcacgc cgcaaggggt tactaagtgt
    11461 ggccgttcga gatttgcggg atgcgctggc ggaccgtcct gaattggagc gggtcagaca
    11521 ggtccgccaa atccgcgaga tgctccagtc agagctgcgg ccccgggaat cggcggccgc
    11581 gcaataaccg gttttttgaa acttacttgc cgctcgtaaa ccgtttctcg atttctttcc
    11641 agttgagaac gttccaccac gcctccacat actcggcacg gcggttctga tacttcaggt
    11701 agtaagcatg ctcccagaca tcgagtccca tgaccgggag cttgccttcc atgatggggc
    11761 tgtcctgatt ggcggtggag agaatgtcga ctttatttcc atccttcacc agccacgccc
    11821 agccggaacc gaaacgggac atggccgccg cattgaattt ttctttgaac gaatcgaaac
    11881 tcccgaacgt tgcgctgatg gcatcggaga gttcgccagt cggtttgccc cccgacttcg
    11941 gagccatgat cgtccagaac atggagtggt tcgcatgccc gccgccgtta ttgcgaacag
    12001 cggtccggat attttccggg acgatcgcga ggttgttcgc cagcaactcc tcaatgctct
    12061 taccggccag gtccggagcg gattcaagcg ccttgttcag gttgtttaca tacgcgccat
    12121 ggtgtttccc gtggtggatt tccatcgtca tcttatcgat gtggggttcc agtgcatccg
    12181 gaggatacgg cagggcggga agtgagaatg acatgttgcc tcctttgagc tttgttttga
    12241 cctacgttgt tccagtaatg ttagatgaca ctcacgcgaa aaagctgctt gctttttcgt
    12301 aagcacccgc cagacggaac atatccgcct cgccaaagtg ccttgtcatg agctgcatcc
    12361 cgacagggag cccttcaaca gtcttaccac acggcacgct catacacgga ataccggcca
    12421 ggctgccggt tagggtgtag atgtccgaca gatacatttc catcgggtcg tctgtttttt
    12481 cacccagcct gaaggcggga gagggcgaaa caggggcgat aatcgcgtca acgctcccga
    12541 aagcgtctgc aaagtcgcgc gtgatcaacg tgcgcacctt ttgcgctttg agatagtacg
    12601 cgtcgtagta gccggcgctc agcacatacg tacccaacat gatgcgcctc ttgcactcca
    12661 gcccgaaccc ggcaccgcgc gtatttctgt acatgtcgat gagggtatcg ccggaagtgc
    12721 gcgcggtgta tcgcacgcca tcatagcggg ccaggttcga actggcttcc gcagtgcaga
    12781 tgatgtaata gcagccgacc gcataggcgg tgtgcggcag actgatgtcc cgtacttcac
    12841 agccgagctt cttcagcgtc tccacgccct gcaggataag atcgcccgtt tcgctggaca
    12901 aatccctgaa gtattcgcgc ggaaggccga tcttcatgcc ttctacgccg ccgtccatat
    12961 caaccggata atccggaacg ggagcgaaag cggacgtcga gtccagagga tcgcgcccgg
    13021 cgagcacccg gagcagcgtg gcggcatcct ttacattgcg tgcaaacggt ccgacatgat
    13081 cgagcgagct ggcgaatgcc gtcaggccat agcgcgacac ccgcccgtag gtgggcgtga
    13141 ctcccaccaa accgcaaaac gatgcgggct gccggatgga gccccccgta tccgagccca
    13201 gcgccacgac agccgtaccc tgcgcgacag cagcagccga cccgccgctc gaaccgccgg
    13261 ggacgcggtc gagagccacc ggattccgca cgggcccgaa ggctgagttc tcgttggaag
    13321 atcccatcgc gaactcgtcg caattcgtct tgccgatgag gatgccccca gcctgttcca
    13381 gccggccgac agcggtcgca tcatacggag gaacgtattc agccagcagt ttcgaaccgc
    13441 acgtcgtgcg aacacccttc gtgacaatca catctttcac ggcgataggc acgccgccca
    13501 acggcccgat gttttcacct cgcgcgattc cttcgtccac gcgcgcggct gcctcgaggg
    13561 cgcgttcggt ggagaaatgc agataggcgt tggttttcgg attctcggcc tgggcaaact
    13621 cgagagcctc gcgagtgagt tccaccgcgg aaaacgcgcg ttccttcagt ccttcgcgaa
    13681 cctgatcgat ggagagggtg gcgatattca ccgctcgatc accctcggca ctttgaaatg
    13741 gccggcaccg gaaagcggag cgtttgcgag agcggcctct tcaccgagcg gcgtccgttc
    13801 gacatcgtcc cgcaaagtgg cggtttctcc ggcttcatac agcacctggg ccatgggctc
    13861 cacggccgac gtatccagtt cgttcagctt gtccatgtgg gtcagaatct tatccaactc
    13921 ggccgccatt ctgcccaact cttcatcggt aagcctgaga ttcgccagtt cggcgacgta
    13981 acgtacttcc tgttcgctga tcttcaagcg gaagccctct tgcgcgccat cttatatgca
    14041 tgccggagcg acgggtcgcg gatcccgtcc gcttcgtccc ggaccggcgc gacgccggag
    14101 actctctcct ccctttgagg catgcggcgg ggtacggcga tccggaactg caaatcggtg
    14161 aaaagtcccg ggccggccac ctgttccatc ctcgcaagaa tctgccgcct cagtgtgaac
    14221 aactgccgct gccattcgaa gtcttcgaca tcgatgacga gcttcttatc caccacggca
    14281 gccacattgc tgtgcgcggc aataattttc ccaaccgcga tggaccaggc acgcgttgca
    14341 acagcctcag gagaaacgtt tttcatcctc gcaatgagtc taccggcgcg ttccatggtg
    14401 ggaacatgta ttgtagcatt gacctttgcg cactgacgaa tgaatctgat tcttgcttcc
    14461 gcttccccac gccgcagcga gcttttagca tcggccggca ttcgtttcac cgtccgagtc
    14521 tcccaggtag aggagcggca tgtgcccggc gaggacgcgt ggcagtatgt caaaagactt
    14581 gcggaagcca aagcccggac cattgaaagc acacccggcg aagtggtgct gggcgcggac
    14641 accgttgtgg tcgtggacga tcacattctc gaaaaacccg cggaccatgc ggatgccgcg
    14701 cgaatgctgc ggcttctctc cggacggcgc caccaggtga ttacgggaat ctgcctgcgc
    14761 ggcctcaatc gcattaccgt ggatgccgaa acaacagagg ttcgttttgt atcgctctcc
    14821 gaggccgaga tcgacgacta cgtgcgcacc ggcgagccca tggacaaggc gggcggatat
    14881 gccatccagg gtctggcatc gaaatttatc gatcgcgtcg aaggctgtta cttcaacgtt
    14941 gtcggcctgc ccgtcgcatt ggtataccag aagctaaagg agttcgaagg aatgcatgag
    15001 taagagggca accgtcggcg tcacctaccg gcttgaagaa aaactgaagc cctatgaaga
    15061 cgcgctgcgc gccgccggcc tggacgtcgt gcgtattcaa gccgggtctt cttcaaagct
    15121 cgatggatta ggcggtctcg tgctaaccgg gggcacagat atagacccct ctttctacgg
    15181 cgagaagcct gcgcccgaaa cacaaacgcc tgaccgtgaa cgcgacgaga tggagttgag
    15241 cctgcttcag caggcgctct cggtcgacct gccggttctg gcgatttgcc gtggaatgca
    15301 gttgttcaac gtcgcacacg gtggcgcact gattcagcat ctgggtgaag gcagtccgca
    15361 cacccgggga cgcgacgtcg atcacgtaca ttcgattatc acggtcgaag gaacgcacct
    15421 gtcgcgcatt ctcggacccg gagagcaggt ggtgaactcc aggcatcatc aggcggtcga
    15481 tccggatcga accggcaaag ggctgatcgt ttccgcaaaa tctccggacg gtgtcatcga
    15541 aggactggag atgcccggcc aacgcttcgc catcgccgtg cagtggcacc cggaggactt
    15601 gttcaggctg gacgcgagat tgttccaggc gttcgccgaa gcggtgtgac ttcagcgcgt
    15661 gccgttactc tgccgatcag gtccaaagac cgatggaccg ctccctcgcg tgcgcggctc
    15721 agaaacactt actgagccgc gcacgtgaga agctgtgaag aaattgaatt tagccacgga
    15781 taaacacggg taagtttcct gttttcaatc tgtgtttatc tgtgtccatc tgtggctctg
    15841 aatctttcac atcttctcag taagcggtcg aggagcttgt tgaaaaaatc attgcagccg
    15901 ccgctccctc gcggtcacgg ctcagaaacg gagccgcgcg cgccagcaag cggtggccct
    15961 acacgaaccg gtacttattc gtcttatcgt cccagaggat cttcttgccc ctcttgaacg
    16021 acagggtagc catgtggccg ccgatggccg cctgctgtcc gatttcagcc ggtgcaatcg
    16081 ctttctcttt gccgagcacg gccatgatga aattcttcac gtgcgcctgt acggcgttgg
    16141 cgtcgttccc cttcgcttca atcctgatct cctggcgcgc ggctactttc tccggcggtt
    16201 tgcctccgaa cttctcgggc cagaaggaga ggaactggcg gctgttcact tcgatggtgc
    16261 cttcgttccc catgaactgc tcgccgtagc caaagtggtc atttccgaaa tagcaactgt
    16321 agttgatgtg gaagcggtcc ggatattcgt aaatggcact gaggcaatcg ggcacttcgc
    16381 ggtcgtcctc cgtccagcga tagattccgc cggaagccat acaggaggaa ggaaccgttt
    16441 tgccgcaaac gaagttggtg atgtcggtct gatgaaccag cagatcggtg gagataccgc
    16501 cagagtaatc ccaataaaga cgccactggt agtagcgctg cttatcgaat gccctgttgc
    16561 tcgccggtcc caggaagcgc ttccaatcgg tgttttgagg atcggcgtcc ggcggaatcc
    16621 ggtaccgcca cgcgggatta tcgtcgaggg agttgcgata ccagaacgcg cgaacgtaat
    16681 gaacgtcgcc gatcattccc tgctccacca tctccttggc tttgatgtag agcgagttgg
    16741 atcgattctg cgtacctact tgcacgacct taccggcttt cttcgccgca ttcacgatgt
    16801 cccacccctc ttcgatggta tgggccagcg gcttttcgag gtagatgtgt ttgccggcgt
    16861 tgagcgcggc caccgccatc tcgtggtgca gatgctccgg cgtacaaata aagaccacgt
    16921 cgatggaaga atcgcgcagc acctcgcgat aatccacgta ggtcttcggc gtgttgcctc
    16981 cctgggtttg aacggcgtct ttcgccctgt tcaggttgcc cgtataggta tcgcacacgg
    17041 ccgtgacggt gaccagttcc ggcaccgcct tgtacatcat gcccataacg tggttgccgc
    17101 ggctccccgt gccaacccag ccaacctgaa ctttgtcgtt cgcgccaagc gcaggcagga
    17161 tggccggacc ggccgcaagc gctactcccg ctgagttccg gatgaagttg cgccgggaga
    17221 gttcatcgga cataagtaca catctccttc attaagggat ttactccttc agaatatcag
    17281 gatttcccac acaggaatct gaaacgatct gactcccggc aacgtcgaaa cggggtgaac
    17341 atcagccggg tggcagtatc tcttcttctc ctactggccg gaaacctgtt tgccggccgt
    17401 ctcgaacgat atgcgctcat cctcgaaggc aatcccgcgg caagacaggc atcgtcctcc
    17461 aaaccgggcc agccgcgttt cgccgaagat caaagacaaa agctggtagc cgcgcaatcc
    17521 aatgtgcggc gcgcgctagc ggatcgcaag atccgggtca cgggagccgt gcagactatc
    17581 gccaatgcgg tgttcgtagt ggcctccgaa gaacaggcag ccgcgctgcg cacgcttccc
    17641 ggcgtggccg gcgtacaact gatgcgcccc gtgaagcgga gtctgaaccg cgccattgac
    17701 ctgatgaacg tctccggagc ctggagcgcg ctccagggcg aaggcaatgc gggcgcggga
    17761 atgagaatcg cgatactcga taccggtatc gaccagaccc atcccgcttt tcaggatggc
    17821 tcgctgcaaa taccggcggg ttttcccaag tgcgccggac agggatgcga atacaccaac
    17881 aataaagtga tcgctgcgcg cagctacgtg cagatgctgg tgctgcctga agatccggcg
    17941 gattcacggc ccgacgatct ctccccgcgc gatcgtgtgg ggcacggtac ggcggcggcc
    18001 agcgttgcgg cgggtgtgcg caacggcggt ccggcggcaa ctatcaccgg cgtcgcaccg
    18061 aaagcgtttc tcggcaatta caagatcttc ggatctcctg gtgtgaatga tgtgactttt
    18121 accgatgtcg tgattcaagc gctcgaagac gcctacaacg acggtatgga tgtcgccagt
    18181 ctctcactgg gcttcgcggc agtgtgggga ccactcgatt ccggttctgt ctgcggcaac
    18241 tctccagggg tgccgtgtga tctgtttgcc gccgcggtgg aagaggccgc gcaagcgatg
    18301 gtagtggtgg tctcggcagg caacgacgga gatctcggaa acctggcacc cagcctgaac
    18361 acgatccatt cgccggggac ggcgccttcg gcgatcaccg tgggtgcgat ctataactcg
    18421 catgagtttt tttcgagcgt gcgagcctcc gggctggacc gcatcaacgc gctgtttgga
    18481 aacggaccga agcccggcgg ccccatcagt gcaccgctgc gagtggtttc gagaagcgaa
    18541 gaccagaaag cctgcacgtc tcttcaaagc ggctcccttg ccggcgcgat cgccttgatc
    18601 gagcgcggcg attgcggttt tgacaccaaa gtcatcaatg cgcagcgagc cggtgcggtg
    18661 ggagtaatcc tccatcaatc gcaaggatcg aatttcgttt tcccgcctac cgggctttct
    18721 gaaaccggca ttcccgcggt cctcatcggc tacgatgccg ccacgcgtct ctccagttcg
    18781 gctgcatcga acgcggattt ccaggtcacg ctcgacccgg ccctcgtcga agtgaatgcg
    18841 acgtcgaacc aggtggcgta tttctcttct tacggacctt ccatcgctac gtttggcatc
    18901 aagcccgaga tctccgcaac gggcacggac ctctacatgg ccacacagaa gtatgacccg
    18961 aacggcgacc tgtacgatgc gacgggatac accaatgcgc agggcaccag tttcgccgcg
    19021 ccgatcgtag cgggagcagc tgcgctggtg atgcaggcgc acccgaattt ttccgtggcg
    19081 gacgttaagt cggccatcgt gaacacgact agcgcggatg tcagcgatat cgacggggag
    19141 ggacgtgtcg tgcccgcgcg cgtggcagcg gtgggcgcgg gcaagctcga tgtgagcgcc
    19201 gcggtgcaaa cacagatcac ctcggacgtc gccaccatct cgttcggtgc gatcggagcc
    19261 ggctcgtccg gcgtttcgcg cagctttcgc ctcacgaact ccggcacgga ttcggtacag
    19321 ctttccgtca gggtggcgga acgggacacg gacgccggcg gaagagtgtc gatcgtcggc
    19381 gaaacgtcgt ttaccctggc gccggggaca agaaccacca atatcaacgc gcgcctggac
    19441 cttacgcagc gcaaccccgg ctcttacgaa ggcttcgtca acgtgatgca gggttcgcga
    19501 gccgtactgc gaattcccta cgtctactac gtcccgggca gcggaccgtt caacatcatc
    19561 ccgcttacag gtttcgattt caccggaata gcggaccaga gattacccgg acggctcact
    19621 ttgaaagtga tcgatcgttt tggcattccg gtgccggatt tgcctgtcca gttccggccg
    19681 gcgaatgcaa ttgacacggc cacggagacg accgactcgc ttggcatcgc agaggcgcag
    19741 gtttttctcg gcccacaatt gggcgaacag gaattttccg ccgacgtggg cggcagtacg
    19801 gtttactttc cgggaaaggc cattcaacgt ccggcgatct tcaccaacgg cgttgttaac
    19861 gctgccagca atcaggtggg gcagggtctt gcgccgggct cgttgatttc catttacggg
    19921 tcggcgctga gccaggcgat tcggggcgtt ttcacacctt atctgccggt gtcgctggcg
    19981 ggcatgagcg tcagcttcga caggccatcg gacaacgtaa gcgtgccggg acggctccac
    20041 tacgtcagcc ccgatcagat caacctgcaa atcccgtggg agctcgaagg ctacaacaac
    20101 gcgatcatga agatcagtat cgggaacttt tccagtgggc tatacgacgt tccgctggcg
    20161 aggtattccc cggccttcta tgaatacaac gatgccggga gcggccgcac gctgatcgcc
    20221 gcgctggatg aagggaacgt gctggtcacg ccgtcgcgcc ccgcgcgcaa gaacacggtg
    20281 gtgcaattct acgccaacgg tcttggcccg gtggacaacc ggccggcgag cggcgaattg
    20341 agcccaacct cgcccctggc gcgtacgcgc gtgattccgg aagtccggat cggcggccgt
    20401 cctgcgcacg tggatttctc gggtctggcg cctaccttcg cgggtctgta tcagatcaat
    20461 gccgtcatac cgggagacgc acccagtggt ttgcagccgg tcgaaatcac catggaaggc
    20521 gttacttcca aaactgcgtt tctgccggtg gagtaaacag agccgtaccc tatgaaccga
    20581 atctggatcg ccgtattagc gctgccgctg gcggcgcaga ttgttccgaa ccgctacatc
    20641 gtggagttga gcggtgagcc cgcggccacc tatgccgtca agacgaaggg caatttgcgg
    20701 gcggcgctgg cagaccggcg tgccgcgatt caaagcgagc agcttcctgt acggcaggcc
    20761 gtgcagaaca ccggaggcac ggttctcgcc agcgtcgata cggtagccaa cgcgctcgtg
    20821 gtagagattc cggactcgca agccgccagc ctgaaccggc tgcccggtgt gctgcgcgtg
    20881 cacaaggtct gggaaggcaa gctttttctc gaccatgcac ttccgttatc ccgcgttccg
    20941 gaagcctgga tggaagccgg cggcgaggat cgtgcaggcg ccggcatgaa gatcggaatc
    21001 atcgatacgg gattggacgc ggaccatccc gctttcttcg attcgaacct gcagcccgcc
    21061 ggtgtgcccg ccgcaggctt cgaaaacaac cccagaatca tggtggcccg cagctacgat
    21121 cgcgggcccg tcctttccgg ccaaggcgcg cgcgatttga atggacacgg caccggtgtc
    21181 gccatggtga cggcgggcgg acgcacagcc gctccgctgg caaccatttc cggcgtcgcg
    21241 ccaaaagcgt atctcggtat ttaccgcgtt ttttcgggca actcgggaag tttctcgagc
    21301 gatctgctat tgcgcgccct ggacgatgcg gtcgccgacg gtatggatgt agtgaacctc
    21361 agcctgggcc ttaccgcgcc gctgcgccca tccgtggacc cgttctacaa cgcggtggag
    21421 cgtgccgcgg cagcgggcgt catcgtcgta gccgcggcgg ggaacgaagg accgtaccct
    21481 ggcagtgtcg gacccccggc gagcgcgcct tccaccatcg ccgtcggggc gaccgccaac
    21541 agccggatct ttgccgcctt cgtccaaccg gacggcgaca cgccgatcac agcggaacca
    21601 gggagccggt ccgcaacagg tgcggcggtc acagggccca tgatagacct tgccgcgatc
    21661 gattcttcgg ctctctcgtg cagcgcgcta ccggcagacg ctctgtccgg acacattgtc
    21721 ttgattctcc ggggtgagtg cacttttgag acgaagctga acaacgcgca gaatgcgggc
    21781 gccctcggcg ctgtcgtcta tacgcaggcg gaagatccag gcctgtttcg aatggagacc
    21841 ggcagcgttg cgttgccggc cctgtccgtc agccatgagg acggtctccg gttgaaggct
    21901 ttgctccagg aacagccgga attaaccgcc acgctccagt tgtcgcgggc cgcggtgccg
    21961 gccgatccca ataacctcgc ggatttttcg agtaccggtc cgaacacgga cgccggcatc
    22021 aagcccgatc tggtcgccac cggaaccaac gtgtatactg ccgccgaaaa gaccgaccca
    22081 agcggcgaat cttacgatgc cagcggtttc ctcgtgcaga gcggaaccag tttttccgct
    22141 cccctcgtct ccggagcagc cgcagtcctg aagggtgcac gtcccgggtt gaccgccgcg
    22201 caataccggt ctttactgat caactcaacg gcccggtttc tcttcaccgg tggccaggca
    22261 gcttcta
//
LOCUS       DASTMK010000443         5899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_469352, whole genome shotgun sequence.
ACCESSION   DASTMK010000443 DASTMK010000000
VERSION     DASTMK010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469352"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFQ79_18950"
     CDS             <1..199
                     /locus_tag="VFQ79_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEU0141809.1"
                     /translation="TFQKAQGHLKLLNVSRRNIELLVLTKLETVFEVFADEQDAVNSF
                     FPNREIRRFDILTFIQQHKQE"
     gene            209..1513
                     /locus_tag="VFQ79_18955"
     CDS             209..1513
                     /locus_tag="VFQ79_18955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0141810.1"
                     /translation="MKLVVIGGVAAGMSAAARARRLDPSCEITVLEKGDTVSYSACGF
                     PYFIDGRVRSLDDLVAYTADEFRRERNIDVRTSAEVVSISHARREVSLAGGDRVRYDR
                     LVIATGAGPDAKNITGATLPHVFHLVSLGSTRALDEFLKSRNPRSAVIVGGSYIGIEA
                     AEALRSRGLAVTLVEKKPYLLSRNNLQLTGILKAHLEKFRIEVRLETAVNAITPSAAG
                     EFSCDLVVLATGLKPNVELAADAGIELGRTGAIRVNERMETNLGGVFSAGDCAEAYHV
                     VTGAPVWIPLGTTANKMGRVAGANAVGKRERFGGIAGTAIVRACGLGVAVTGLSPAEA
                     KREGFVAVDCWIHSRDRASYFQGRPTSVQLVADRNTHRLLGGAVVGEGGVEGRIGIIA
                     AAIQARMKVEEFQDLDLAYAPPFAPVWDPVLIAAQQLLKALP"
     gene            1590..2087
                     /locus_tag="VFQ79_18960"
     CDS             1590..2087
                     /locus_tag="VFQ79_18960"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0141811.1"
                     /translation="MGFGKHRRILEKGSRAPDFSLADLQGEVVSARQILSRGPALFAF
                     FKISCPVCQLTLPFLERFHRQLDRGVLQVFGISQNDEGATRRFGREFDLTLPMLLDTS
                     ASGYPVSNGFGISQVPSMFLVEQDGTIAWASEGFSKQDLEDLGHRFAINPFRADEEVP
                     AFRAG"
     gene            2158..2667
                     /gene="nuoI"
                     /locus_tag="VFQ79_18965"
     CDS             2158..2667
                     /gene="nuoI"
                     /locus_tag="VFQ79_18965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU0141812.1"
                     /translation="MASKIQDALSSAAALAKGMGVTLKEMMSPTITEDYPDVPPKFQE
                     RYRGVHVLQRDENGLEKCVACFLCSANCPSDCIYIEAAENTDSLRISGGERYAQVYNI
                     DYSRCIFCGYCVEACPTDAITHGHGFEIASYDTSTLIWRKEQLLAPVPSRETQAGINP
                     PSPGARPPV"
     gene            complement(2630..3745)
                     /locus_tag="VFQ79_18970"
     CDS             complement(2630..3745)
                     /locus_tag="VFQ79_18970"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEU0141813.1"
                     /translation="MNILVPNLGSTSLKYQLLDMPSERVLSKGRLERVSDYHDAVKQI
                     DTGGHPIQVVAFKAVLAGPRYRGTFLINDDLMAAMEDFLPAAPAHNAIYLTGIRAFRE
                     TMPGVPLVAAFETEFHTTIPDYAGHYGVPEEWRARSGIRRYGFHGASHAYISERVPEI
                     LGVQAGQIKLVSCHLGGSSSLCAVDGGVSVDTTMGFSPQSGLENATRHGELDIFAVLY
                     MMDRNGWDTEQVRRQLSRTGGLAGISGIAGGDVRDIEKAAAGGNAQATLALELFAYQV
                     RKTIGGYAAAMGGIDAIAFTGGIGENSARLRANCCRGLEFLGVRLDRDANESGQGDRV
                     ISTGDSRVAVVTLATNEELNVARRAFKLVGARQATAD"
     gene            complement(3750..4859)
                     /locus_tag="VFQ79_18975"
     CDS             complement(3750..4859)
                     /locus_tag="VFQ79_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141814.1"
                     /translation="MRVRVLAITFLAGAALLIAQRRELTIDAGTPEGAQLQQIGQESD
                     EAKKLQMFEKFAADHPQYDGIVWVYAQMQPLYIKQGNFQKAMEAGEKVLAADPEDARM
                     AHETLKAAEGTKDPDLILKWSNHGSEVARKIAASTKPGDDEDEEHWKQRVDFAKQFDT
                     YTEYALYAALLQTPDPGKKAMLAETLEKRNPESQYLPQMAGPLFVAYQQSGAKDKALA
                     LAEKTLQKEQTNEDMLLAVADHYMSQKDAGKVLLYSGKLIELMQTKPRPEGTNEEDWE
                     KKKALSLGLGYWMQGMTYMSQNKFVDAEKSLRASLPHIKENEQLMPSALFNLGLANHK
                     LKRNKDAVTYFSQCSKYKSPYQAQAIQNVTALRGR"
     gene            4933..5289
                     /locus_tag="VFQ79_18980"
     CDS             4933..5289
                     /locus_tag="VFQ79_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141815.1"
                     /translation="MFKSICFAALICLLACAPGFAAGSAVAGTWNLTADTADGEMRWT
                     MVVKDDGGKLSGTASASQGDYTMEDPRLDGNKFSFKITFDGAVYRVSMVIDGSRVSGK
                     WSGPSDGGKISGTKRP"
     gene            5323..>5899
                     /locus_tag="VFQ79_18985"
     CDS             5323..>5899
                     /locus_tag="VFQ79_18985"
                     /inference="COORDINATES: protein motif:HMM:NF018828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HEU0141816.1"
                     /translation="MILPSTYSIALLLTLVSMICWGSWANTQKLAGKWRFELFYFDYS
                     FGVLLTALVAAYTFGSMGDGLSFMDNLAISSRRNIAFGLAGGVVFNLANMLLVAAISI
                     AGLSVAFPVGIGLALVIGVVWNYSINPQGNPTLIFAGAGLVTVAIIVDALAYRAHAAA
                     RQKSAASGSRSGASRFWKGILLSIVSGVLMGS"
BASE COUNT         1110 a         1698 c         1750 g         1341 t
ORIGIN      
        1 cactttccag aaggctcaag gccatttgaa gctgctgaac gtcagccggc ggaatatcga
       61 gctgctggtt ctgacaaagc tcgagaccgt gttcgaggtc tttgcggacg aacaggatgc
      121 cgtcaatagt tttttcccga accgtgaaat ccggcggttc gatatcctca cgttcattca
      181 gcagcataaa caggaataac ccgccgcaat gaaactggta gtcatcggcg gcgtagcggc
      241 cggaatgagc gccgcggccc gtgcgcggcg actcgatcct tcctgtgaaa ttacggtact
      301 cgagaagggc gataccgttt cttacagcgc ctgcggtttt ccttatttca tcgacgggcg
      361 agtgcggtcg ctcgacgacc ttgtcgctta tacagcggat gagttccgcc gcgagagaaa
      421 tatcgatgtc cggacctcgg ccgaagtggt atccatctcc cacgcgcggc gggaagtttc
      481 ccttgcgggt ggcgatcgtg tccgttacga ccgcctggtg attgcgaccg gagccgggcc
      541 ggatgcaaag aacattaccg gcgctacgct gccccacgtt ttccacctgg tttcgcttgg
      601 gagtacgcga gcgctcgacg aattcctgaa gagccggaat cctcgctccg ccgtcattgt
      661 tggcggcagc tacatcggca ttgaggcggc ggaggctctt cgctcgcgcg ggctggccgt
      721 gacgctggtc gagaagaagc cgtacctgct gtctcgcaat aatctccagc taaccggaat
      781 cctgaaggcg cacctggaga agttccggat cgaggtccgg ctcgaaacag ccgtgaacgc
      841 cattactccg tcggcggccg gcgagttttc gtgcgacctc gtggtgctgg caaccggcct
      901 caagccgaac gtggagcttg cggcagacgc cggcatcgaa ctcgggcgca ccggcgccat
      961 tcgcgtcaac gaacgaatgg agaccaacct gggcggggta ttttcggcgg gcgactgcgc
     1021 cgaggcatat cacgttgtca ccggcgctcc cgtatggatt ccgctcggca cgaccgccaa
     1081 caagatgggc cgtgtggcgg gggcaaacgc agtgggaaaa cgggagcgtt tcggcggcat
     1141 cgcgggcaca gccatcgtcc gggcttgtgg attaggcgtc gcggtaacgg gcctgtcgcc
     1201 cgccgaagcg aagcgcgagg ggttcgtggc ggtcgactgt tggattcatt cgcgcgatcg
     1261 tgcctcgtat tttcaaggcc gccccaccag cgtgcaactg gtggccgacc ggaacacgca
     1321 ccgtctgctg ggcggcgccg tggtgggtga aggcggagtg gaggggcgca tcggaatcat
     1381 cgccgcggct atacaggccc gcatgaaggt ggaggagttc caggatttgg atctggccta
     1441 tgcgccgccg ttcgcacccg tctgggatcc cgtcctgatc gccgcgcagc agttgttgaa
     1501 agcactgccg taattgcgat cacttttctg agccgcgcgc gcgagcaagc ggttttcgcc
     1561 ctttttcagc agcctgttag actgaaagta tgggcttcgg gaagcacaga cgaattctgg
     1621 aaaagggctc ccgcgcaccg gatttcagcc tggctgacct acagggagag gtcgtgagtg
     1681 cgcggcagat tctgtcgcga ggccctgctt tgttcgcgtt tttcaagatc tcatgcccgg
     1741 tctgccagct tacgcttcca tttctggagc gtttccaccg gcaactggac cgcggtgttt
     1801 tgcaggtctt cggcatttcg cagaacgatg aaggcgcgac ccggcgattc gggcgtgagt
     1861 tcgatcttac gctgcccatg ctgctggaca cgtccgcctc cggttatccg gtgagcaacg
     1921 gatttggcat ctcgcaggtg cccagcatgt ttttggtcga gcaggatggt accatcgcct
     1981 gggcttcgga ggggttctcc aagcaggatt tagaggattt ggggcatcga tttgccataa
     2041 atccgttccg cgcggacgaa gaagtgcccg cgttccgggc cggttgaggt tctaaaaact
     2101 aggctcccgt gcggagctgg gcgttgaagg cacaggatag gacaggctga ataaacgatg
     2161 gcatcgaaga ttcaagacgc gttgagttcg gcggcggcac tcgccaaggg gatgggagtc
     2221 actctgaagg agatgatgtc tcctacgatc acggaggatt accctgacgt tccgccgaaa
     2281 ttccaggaac gttaccgcgg agtacacgtg cttcagcgcg acgagaatgg cctggagaag
     2341 tgtgtcgcgt gtttcctgtg ttcggcgaac tgcccttccg attgcatcta catcgaagcg
     2401 gcggaaaata ccgattcgct tcggatcagt ggaggcgaac gttacgcaca ggtttacaac
     2461 atcgactatt cgcgatgcat cttctgcggc tattgcgtgg aagcgtgccc caccgacgcc
     2521 attacccatg gccacggttt cgaaattgcg agctatgata cgtctacgct gatttggcgc
     2581 aaggagcaac tgctggcccc ggtgccttca agagagactc aggccggcat taatccgccg
     2641 tcgcctggcg cgcgcccacc agtttgaagg cacgccgggc gacattcagt tcttcattcg
     2701 tggcgagtgt aacaacggcg acacgggaat caccggtgga aatcacccgg tcgccctggc
     2761 cgctttcatt ggcatcgcga tccaggcgga ctccaagaaa ctccagaccc cggcagcaat
     2821 tggcccgcaa acgcgcggag ttctcgccga tgccgccggt gaacgcgatg gcatcgattc
     2881 cccccatcgc cgccgcataa ccaccgattg tttttcttac ctgataggcg aagagctcca
     2941 gcgcaagagt agcctgtgca ttgcccccgg ccgccgcttt ctcgatatcg cgcacgtcgc
     3001 ctcccgcaat gccggagatc ccggccaagc cgcccgtgcg agacaattgc cggcgcacct
     3061 gctcggtatc ccagccgtta cgatccatca tgtacagcac ggcgaaaata tccagctctc
     3121 catggcgtgt cgcgttctcc agtcccgatt gcggagaaaa gcccatggtt gtatcgaccg
     3181 atacaccgcc atccaccgcg cacaaagacg aactgccgcc caggtggcaa cttactaact
     3241 tgatttggcc ggcctgtact ccgagtatct ccggcacccg ctcgcttata taggcatgcg
     3301 aagcgccgtg aaagccatac cggcgaatgc cggaccgcgc gcgccactct tccgggacac
     3361 catagtggcc cgcgtagtcc ggaattgtgg tatggaattc cgtctcgaaa gccgcaacca
     3421 agggcacacc gggcatcgtt tcgcgaaacg cgcgaatgcc ggtgagatag attgcgttat
     3481 gggcgggcgc ggccggaaga aagtcttcca tagcggccat cagatcgtcg ttgataagga
     3541 acgtgccgcg gtagcgcggg ccggccagga ccgctttgaa ggctaccacc tggatcggat
     3601 ggcctccggt gtcgatctgt ttcaccgcat cgtgatagtc ggaaacccgc tcgagccgcc
     3661 ccttcgaaag cacgcgctcg gaaggcatat ccagcaactg gtacttgagc gaagtcgaac
     3721 cgaggttcgg aacgagaata ttcaagcggc tatctcccgc gaagcgccgt cacgttctgt
     3781 attgcctggg cctggtaggg acttttgtat ttgctgcatt gtgagaagta agttaccgcg
     3841 tccttattgc gcttcaactt gtggttggcc aggccgagat tgaaaagcgc gctgggcata
     3901 agttgctcgt tctccttaat gtgtggcaac gaagcccgca gcgatttttc ggcgtcgaca
     3961 aacttattct ggctcatata ggtcatgcct tgcatccagt agccgagtcc cagcgaaagg
     4021 gctttctttt tttcccaatc ctcttcgttt gtcccctccg gccgcggctt cgtctgcatc
     4081 agttcgatga gcttgccgga atacagcaga accttgccgg catccttctg gctcatgtaa
     4141 tggtcggcaa cggctagcag catgtcttcg ttcgtctgct ccttttggag cgttttctcg
     4201 gccagcgcca acgctttatc cttggctcca ctctgctgat acgcgacgaa taaagggccc
     4261 gccatttgtg gcagatactg gctttccgga ttgcgcttct ccaaagtttc cgccagcatc
     4321 gcttttttgc cggggtcggg agtctgcagc agggccgcgt acagagcgta ctcagtatag
     4381 gtgtcgaact gcttagcgaa gtctacccgc tgcttccagt gctcttcgtc ttcatcgtcg
     4441 cctggtttcg tggaggctgc gatcttccgg gccacttccg agccgtggtt tgaccatttc
     4501 aggatgagat ccgggtcctt ggtcccttcc gccgctttca atgtttcgtg tgccattcgc
     4561 gcgtcttccg gatcggccgc gagcactttt tcacccgctt ccattgcttt ctggaagttg
     4621 ccctgtttta tatagagcgg ctgcatctgt gcgtataccc acacgatgcc gtcgtactgg
     4681 ggatgatcgg cggcgaattt ttcgaacatt tgaagcttct tggcttcgtc gctttcctgg
     4741 ccgatctgtt gaagttgcgc accctcgggt gttccggcgt cgatggtcaa ttcccgcctc
     4801 tgagcgatca gtaacgcggc gccggccaga aacgtaatgg cgagtaccct tactctcatt
     4861 cgttcaccct ccattgatat tgaacgggtg tatcattata cagaatttgg attcgagtag
     4921 ggatcttcat ctatgttcaa atcgatctgt ttcgcggctc tgatatgctt actcgcctgc
     4981 gctcccggtt tcgccgccgg gtctgccgtt gccggaacgt ggaatcttac ggcggacacc
     5041 gcggatggtg aaatgcgctg gacgatggtc gtgaaggacg acgggggtaa attgagcggt
     5101 accgcctcag cgtcacaggg cgattacacc atggaggatc ccaggctcga cggcaataag
     5161 ttcagcttta agattacatt tgacggcgcg gtttatcgtg taagtatggt tatcgatggg
     5221 tcacgcgtct ccgggaaatg gagcggccca tcggacggtg gaaagatcag cggcacaaaa
     5281 agaccctgac catcgacgga atactccgct tcgtacagtg caatgatact tccttctacc
     5341 tactcaattg cgctcctgct cacgctggtg tccatgatct gttggggatc gtgggccaac
     5401 acgcagaagc ttgcgggcaa gtggcgtttt gagctttttt acttcgatta ttcgttcggt
     5461 gtcctgctga cggccctcgt cgcggcctac accttcggtt ccatgggcga tgggctttcg
     5521 ttcatggata atctcgcgat ttcgtcgcgc cgtaacatcg ccttcggttt ggcgggtgga
     5581 gtcgtcttca acctcgcaaa catgctcctg gtggcggcca tcagcatcgc gggcttgtcg
     5641 gttgcatttc ccgtgggaat cgggctggcg cttgtcatcg gcgttgtctg gaactactcc
     5701 attaatccgc aaggcaaccc caccttgatc ttcgccggcg cgggtctggt gaccgtcgcc
     5761 attattgtgg atgcgctggc gtaccgtgcg cacgcggccg cgcggcagaa aagcgccgct
     5821 tccggttcca gaagtggagc tagccgcttc tggaaaggaa ttctgctcag cattgtaagc
     5881 ggcgtcctga tgggctcct
//
LOCUS       DASTMK010000444        16641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_469375, whole genome shotgun sequence.
ACCESSION   DASTMK010000444 DASTMK010000000
VERSION     DASTMK010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16641
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469375"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFQ79_18990"
     CDS             <1..397
                     /locus_tag="VFQ79_18990"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEU0141817.1"
                     /translation="THPIAVMPAAGDSVPFVVFMARENSSDSAEIPTHAVRVFFDPTN
                     GRHLETRRPPPNRTAGDWIIWSMKPFHFGTEWGFAMKVVWALLGLSIPVLTITGALMY
                     WNRSLSKLWRKHKRPACTLPVVREHQEVA"
     gene            complement(399..4928)
                     /locus_tag="VFQ79_18995"
     CDS             complement(399..4928)
                     /locus_tag="VFQ79_18995"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0141818.1"
                     /translation="MHSSIPSVRGEVLHANSAALRGEADFQDELGSVNLDSQRIAALR
                     RHAHETASSWVDAGRYGGKVLKAEHKRLRALLSSHALTGEPFDAETRAWLLENYRLIR
                     TALRDAGHPDRDMAEHPHVSSAGGASVPRAYALARSFLTSADFVYRPDALAVYVGAVQ
                     DERPLEMGEIWALKPMLQLVLLQELAATLERIANADSASGETGNPAAGRLISSLRAVA
                     QTDWKSLFEQVSLVERILRSDPSLTYPRMDYESRDMYRNAIAEMATHSRAPETDIAAT
                     AVRLAKHAAEEDAPTTRIRERHSHVGFYLVDLGLPALQREIGYVPPFARRVRDAILNS
                     PNGFYLIGVELSTMLIAFLLLSQLETFTPIVAGFLLLITPATQAAVDFMNSLTTFLLK
                     PRPLPKLDFSEGIPDEFRTMVAVPTLLLNEQQVEETVNELEIRYLGNPDRNLYYALLT
                     DAPDSDRPFDEKEELANFCSELIRGLNRKYGGGGGRFYLFHRHRVFNPSEGAWMGWER
                     KRGKLLDLNKLLRNDFDSFPVKVGDMSILPSIQFVITLDSDTQLPRDSAHKMVGTLAH
                     PLNRAIIDPVTNTVVAGYGILQPRIGISVQSAGRSRLAALYSGQTGFDIYTRAVSDVY
                     QDLYGEGIFTGKGIYDVDTLRAVLDHRFPCNALLSHDLIEGAYARSALVSDIELIDDY
                     PSHFSAYSRRKHRWVRGDWQIAQWLFPRVPDYDRNSVPNPVTLASRWKILDNLRRSVI
                     DPATFTLFLTGWFYLPGKPWYWTAATLALMLMPVYTQLLFSLLRFGDFRRIVPFAKET
                     GSAFLKGHFHVLLNLVFLAHQTLVMLDAIIRTIVRNRFTHRRLLQWETAAQAEAGMKR
                     RTPVDRYLSWIPYVSLLIAGLMALFKPESLPFAGPILALWFASPFISYWLNRAPRVRQ
                     PKLSPDDKKFLRLTALRTWRYFRQFSGPESNWLVPDNVQENPQVIAHRISPTNLGLLL
                     NARQAAVEMGFLTVPEFVRDSERTLASMRRMSRLKGHFLNWYDTRTLEPLEPLFLSTV
                     DSGNLAACLWTLKQGCLSLKSRPLFPASLWQGLREHIHILDEVAPSGVPGLRACVSGL
                     GSGALEWIRELDELERGACALERELGESPEAAWWAHELCERVRAIRSVVERFAPWLLA
                     PAGELVEALEAGTADNLTLDNLTGRVRTIRCHLEERERFQSLSAADERFLAALRSRIP
                     HAAQEADILLSALSGIAEQAESLVRGMDFRFFYHPRKKLLSVGFKVDEGRLMNSYYDL
                     MASEARIAAFVGIAKGDIPQESWFHLGRAQTLYRGERVLLSWTGTMFEYLMPALWMRA
                     YPATLMWKSMVSAVEAQQKYARGKGIPWGISESAYSRTDAAGNYQYHAFGLSGLALKF
                     SSQEALVISPYSTFLALLTHASGAVRNLRDMAARSWCGPLGFYEAIDFTSDSSGQAVP
                     CWMAHHQGMSLLAVCNLVSKYPFRQFFHAEPRVMATELLLQEKAPAVPIAHDDLETEV
                     VAAAGVSAPAAETAG"
     gene            complement(4934..6757)
                     /locus_tag="VFQ79_19000"
     CDS             complement(4934..6757)
                     /locus_tag="VFQ79_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0141819.1"
                     /translation="MPLDSAQTFWGGWKKRLDALYTIPPVLRIVWESGPAVVSAGLTA
                     RLIAALIPITMLSISRLIIDSVVASTRGEPLIPLFWWLVAAEFGLAALGGILGRSIGY
                     FDSLLADQFTRHVSVRVMEHASQLDLASYEDPVFYDKLERARVQATDRIAMIQAFGVM
                     LQQAIVVITLAGSILTFSPWLLLILIACVVPAFLGESHFAFLGYSLNFNQTPVRRQMD
                     YLRVLGASKESAKELKLFGLSSFFTGRYAELSDVIYKQNVALAKRRLWAGSMLSLLTS
                     AGYYGAYAYVIYRTVTGDLSVGTMTFLAGAIAGASSNIQSIFATFSSIADQALFLTDL
                     IEFFAMQPKVRSKPGALPAPRPIRDGFEFRNVSFAYPGNSRLVLDSLDFRIEPGQRIA
                     LIGENGQGKTTIVKLLTRLYDPVSGQILLDGIDLREYDIENLSREIGVIFQDFMRYEV
                     SAAENIAMGRVEERNHLEKLESAARKSLADAVIRRLPRGYEQILGRRFEGGLDLSGGE
                     WQKVALARAYLRDAQLLVLDEPTASLDARSEHEVFQRFAELTHGKMALLISHRFSTVR
                     MADRIVVLENGRITEEGSHSRLMALGGRYSEMFELQAASYR"
     gene            7055..8320
                     /locus_tag="VFQ79_19005"
     CDS             7055..8320
                     /locus_tag="VFQ79_19005"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0141820.1"
                     /translation="MKAATGLSSLAVGAPLRAQTRMDLIPHAQRSRQALLDEIHRRSC
                     HFFYDQVNPELGLVHDRAIHTGREQRAVSSIAATGFGLSALCIADQQRYFAAGSIRRR
                     VQATLEYLASEAPHEHGFFPHFLNIRTGERIWGCEYSSVDTAWLLCGVLHCRATFADS
                     GIQALADEIIGRVDWRWMLNGGPTLSHGWTPEHGFLPYRWDSYSELMAMNLLAVSTGT
                     TTLPGESWHAWARPFRNWKGAEYIDSPAPLFVHQFSHAWFDFRARRDRYADYFANSRL
                     ATEAHQEYCAGLSAQFPWYSMDMWGITASESETGYVAWGTPAAPVPPNGTLVPCAAAG
                     SMPFLPDQCTTVLETMLERYGNKVWTRYGFVDAFHPGKGWYSPDIIGIDVGITLLMIE
                     NARTGSVWNAIMSTVEAPRAFDIVGLQKA"
     gene            8446..10116
                     /locus_tag="VFQ79_19010"
     CDS             8446..10116
                     /locus_tag="VFQ79_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU0141821.1"
                     /translation="MNTRRFLAFLSITAFLTATPLAMAAEKKAAGAERETLAKPMSEK
                     ERRKQQERLRKELEGPYRKWLNEDVAYIITDEERAAFKRLSTDDEIEQFIEQFWLRRD
                     PSPDSIENEYKEEHYRRIAYANERFASGIPGWKSDRGRIYITFGPPDENESHPSGGSY
                     QRPMEEGGGTTSTYPFEKWRYRWIEGIGTDVNIEFVDPTMTGEYRMTMDPSEKDALLM
                     VPGAGLTLMEQMGLASKADRFSRTDGTRLGVGDTPLPSRMNQFERLEQYAKLQKPPSI
                     KFKDLEAAVSSTIRYNTLPMDARLDFIRMTNSTVQTNITLQFDKKDLQFQQKDGVAKA
                     IVNIYARITSMTRRPINVFEDVVTVEVPSELLQQASKGSSVYQKSIFLSPGTFRLNVV
                     AKDVVGGNMTNYETAIHVPRYEDEKLGASTLILADTIEKVPTRSIGTGQFVIGTSKVR
                     PRLNETFRRDEKMGIYLQLYNFQPDEQTQKPNAIVEYEVIQDGTNKKVFGYTEEVKGM
                     EGASATQVTIEKLLPLQTMEPGKYTIKLKVTDKNTDQVLTPEATFTIT"
     gene            10165..11949
                     /locus_tag="VFQ79_19015"
     CDS             10165..11949
                     /locus_tag="VFQ79_19015"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141822.1"
                     /translation="MRSRKHIVAAVLAGSLFWPGGAVFAAAPDKLSGSLVGFVSDTAG
                     IPQMGATVLLFNRSERLVNKTLTNDKGAFGFDSLVPDLYSIRVTLASFVPAMKAGIAI
                     QPGARSFLSINLASVLSSIELVYTAPGQGAIMNDDWKWVLRSSMSTRPILRIVPQVDI
                     SDPRSKSSTTAVLSRTRGMVRLSASDMGTVSTGASEPDLGTAFALATSLMGKSELQFS
                     GGVGYSPGGGTPSAGFRTSFSRNNATGMGPEVALTMRQMFLPARAGLAFFTGRSGAAP
                     ALRTMSVSASDRQKLGDSLELEYGASLEAVSFIEHLNYFSPYARMSYAPSDTSTFLVG
                     FSSGVPPATGMEWPTDDFQGDLAVLSAFPRVSLRGGTASVQRSENVEIGYRRKSGSRT
                     YAVGVYRERASNLALSALAPAGVYGADLLPDLFSSSGSIFNIGDFERFGYVVSLTQNL
                     GDNLSASAAYGNREVLAPPMMDSPGAGQLRSSFDRRRSGWVSATLAGTSPWTGTRFVT
                     SYEWTDYAMLAPMRRDLLQHLSSEPGWNVRLRQPIPSIPGMRGRLEATAELRNLLAQG
                     YVPVSAQGKRLLLVPSPRSIRGGLSFIF"
     gene            complement(12217..13515)
                     /locus_tag="VFQ79_19020"
     CDS             complement(12217..13515)
                     /locus_tag="VFQ79_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU0141823.1"
                     /translation="MAEKMNIAVFVDYDNIEIGVKSTLRRGFDVSLPLEALKERGDII
                     AKFAYANWARQEGATRQMAENAVQMVQRIPSPRGDKNAADINLALDALEMAYTHSHVN
                     AFAIVSGDSDFIPLVNKLKEFGKTVYVVGGRAFTSTILQQNCHEFISYESLLDDNKSP
                     GAGVKSEERQQHHPRSPRQRPTPLDIAQAMPLLERALQVLDRRGVQPQLGLLKSTMLQ
                     LDSTFSEKAYGGSSFSDFVDKLKKADFVEVSGPEGRYTIRKKSSAEVPENALLHPEEA
                     LPSLRDVLETHRMEIENGAPADDLATWMHAEHPSFDPKAYGFQEFAEFLNYAQDKTVV
                     RVEPDEEKGLMVYLGAEFYPPALPEREAEPAEPIEEQEEPQPIVEGQPSFLEPIPKPK
                     KTTTTRRKQTKSTAKKRTTGTTSRTRARKNPHPTQEHPAF"
     gene            complement(13543..13944)
                     /locus_tag="VFQ79_19025"
     CDS             complement(13543..13944)
                     /locus_tag="VFQ79_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU0141824.1"
                     /translation="MDAPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EMIVNAASQEDVRVIGLSILSGAHNAIVPRIMDLLREHEMTDVIVVVGGIIPDEDVQE
                     LKKHGVAAVFQPGASLGEIVEFIRSSVKQNA"
     gene            complement(13966..15582)
                     /locus_tag="VFQ79_19030"
     CDS             complement(13966..15582)
                     /locus_tag="VFQ79_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU0141825.1"
                     /translation="MSKSLGAGPDAAVRSFCTLSDIPIAPVYGPEDWKDFDAARDLAR
                     PGEFPYTRGIHESMYRGKLWTMRQFSGFATPEETNARYLYLLEQGQSGLSVAFDLPTL
                     MGLDADDAASLGEVGKCGVSISSLEDMETLFRGIPLDTVTVSMTINSPAPVIWAMYLA
                     VAEQQGVSWSALSGTLQNDILKEYIAQKEFLYPPRPSMRLVTDVIEYAAKYVPRFNPI
                     SISGYHIREAGSTAVQELAFTLRDGIEYVEWALERGLGVDDFAPRLSFFFNAHNDFFE
                     EIAKYRAARKLWAHIMRDRFGARDERTMKLRFHAQTAGCSLTWQQPYNNVVRTTIQAL
                     AAVLGGAQSLHTNSLDEAYALPSEQAVTIALRTQQVLAYESGITSTPDPLGGSYYLEA
                     LTLEVEKAARRYIEQIDGMGGMIPAIEHGFPQREIADASYRYQCSIEKKERIVVGVNA
                     FTGGGEEIELLQVDENAQSRQSEKLARLRARRNAAAVQSALDSLKRAAADGANTMPFI
                     LDAVRAYATVGEICGAFRDVFGSYRERSVL"
     gene            15643..16293
                     /locus_tag="VFQ79_19035"
     CDS             15643..16293
                     /locus_tag="VFQ79_19035"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU0141826.1"
                     /translation="MSEKMFNEPRLALNRIYTRRGDSGETALTGGQRVSKTSPRIECY
                     GTVDELNSFVGMACCSIRKAAPAEPRLAELERILVRVQHELFNLGSILSTLPADVHPK
                     QPRITLREVEQLEQDIDRMNAPLPALRSFVLPGGTRANTELHACRTVCRRAERVCVHL
                     AQQEEIPPECIHYLNRLSDAFFVWSRWVNFVMGVPELLWEPNRAASGLEPDSPPEP"
     gene            complement(16214..>16641)
                     /locus_tag="VFQ79_19040"
     CDS             complement(16214..>16641)
                     /locus_tag="VFQ79_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141827.1"
                     /translation="RLVRFQLAALAALLRSWTADRTGLPDSGFLRAVAANGAAGLKNS
                     IGSIALHPDALEFSARRWFGERHYRIPLSSISEVAFRRRILVDELTLCRGRGEQRYYF
                     FKDSGRANEELFQAMVRAGCPVRDRKQRDWAPTGAQVRP"
BASE COUNT         3466 a         4943 c         4968 g         3264 t
ORIGIN      
        1 tacgcacccg attgccgtca tgccggccgc aggcgatagt gtgccgttcg ttgtcttcat
       61 ggcccgcgag aattcgagcg atagcgccga aatacccacc cacgccgtcc gcgtattttt
      121 cgatccaacc aatggccggc atctggaaac acgcaggccg ccgcccaatc gcacggccgg
      181 cgactggatt atctggtcga tgaagccctt tcatttcgga accgaatggg gattcgccat
      241 gaaagtcgtt tgggcattgt tgggcctgtc tattccggtc ctcaccatca ccggagctct
      301 gatgtactgg aaccggtctc tcagcaagct ctggagaaag cacaagaggc ctgcttgcac
      361 gttaccggta gtccgcgaac atcaggaagt agcctgactc aaccagcggt ctcggccgcc
      421 ggcgcggaca ctcccgccgc ggccacgact tccgtttcaa gatcgtcgtg cgcaatcgga
      481 acggcgggcg ccttctcctg aagcagaagc tccgttgcca ttacccgagg ttccgcgtga
      541 aagaactggc gaaatggata ctttgagacc aggttgcaaa ccgccagcag gctcatgccc
      601 tgatggtgag ccatccaaca aggaacggcc tgtccggaag agtcgctggt gaaatcgatg
      661 gcctcataga aacccagggg gccgcaccag gaccgcgccg ccatgtcgcg caggttccgc
      721 accgcgccgg aagcgtgggt cagcagcgcc aggaacgtcg agtacggcga gataaccagc
      781 gcttcctgtg agctgaactt cagcgcaagg ccgctcaacc caaacgcatg gtactgataa
      841 ttgccggcgg catcggtgcg ggaataagcg gattcggaaa taccccacgg tatccctttt
      901 ccccgcgcgt acttctgctg cgcttcgacg gcggaaacca tgctcttcca catgagcgtc
      961 gcgggatagg cccgcatcca gagggccggc ataagatatt caaacatcgt gccggtccag
     1021 gacagcagga cacgctctcc gcgatagagc gtctgcgccc ggcccaggtg gaaccaggat
     1081 tcctgcggga tatcgccttt agcgattccg acgaacgccg cgatgcgagc ttccgaagcc
     1141 atcaggtcgt agtaagagtt catgaggcgg ccctcgtcga ccttgaaccc taccgaaagc
     1201 agcttcttcc ggggatgata gaaaaagcgg aaatccatgc cgcggaccag cgactcggct
     1261 tgttcggcaa ttccggacag cgcgctgagc aggatgtccg cctcctgcgc cgcgtgaggg
     1321 atgcgagacc gcagtgccgc aagaaagcgc tcgtcggcgg ctgacagcga ctggaagcgc
     1381 tcacgttcct ccaggtggca gcggatggtc cggaccctgc cggtaaggtt gtcgagggtg
     1441 agattgtccg ccgtgccggc ctcgagcgct tcgaccagtt caccggcagg cgcgagcagc
     1501 catggcgcga atcgctccac aacactccgg atcgcgcgca cacgctcgca caactcgtga
     1561 gcccaccagg cggcttcggg agactcaccc agttcccgct ctagagcaca agcgccacgt
     1621 tccagctcat ccagctcccg aatccattcg agcgcgccgg acccgagacc gctcacgcac
     1681 gcccgcaatc cggggacacc cgacggagca acctcgtcga gaatatgaat atgctcccgc
     1741 aggccctgcc acagcgaggc cggaaacaac ggtctgctct tcagcgaaag gcagccctgc
     1801 ttcagagtcc agagacaggc agcaaggttg ccgctatcca cggtcgaaag gaagagaggc
     1861 tccagcggtt ccagggtgcg ggtatcgtac cagttcagga aatgcccttt cagccgcgac
     1921 atgcgccgca tggaggccag cgttctctcg gaatcccgaa cgaattcggg aacggtgaga
     1981 aaacccattt cgacggccgc ctgccgcgca ttcagtaaca ggccaaggtt cgtaggcgaa
     2041 attctgtgtg ctataacctg cgggttctcc tgcacgttat cgggtacgag ccagttcgat
     2101 tccggtccgc tgaactggcg gaaataccgc caggtgcgca gcgctgtaag acgcaggaac
     2161 ttcttgtcat ccggcgacag tttaggctgc ctgacacgcg gcgcccggtt cagccagtag
     2221 gaaataaacg gcgaggcaaa ccagagcgcg agaatcggtc cggcgaaggg cagcgactcc
     2281 ggcttgaaca gcgccatcag tccggcgata agaagcgaca cgtaggggat ccaggaaaga
     2341 taacgatcca ccggtgtgcg gcgcttcatt cccgcttccg cctgtgcagc ggtttcccat
     2401 tgaaggagcc tgcgatgggt gaaacgattc cggacaatgg tccggataat ggcatcgagc
     2461 atcaccagcg tctggtgggc aagaaagacc aggttcaaca gaacgtgaaa atgccctttc
     2521 agaaacgccg agccggtttc cttggcaaag ggcacaatgc gccggaaatc accgaagcga
     2581 agcagcgaaa acaataactg cgtgtatacc ggcatcaaca tcagcgccag cgtggcggcc
     2641 gtccaatacc agggtttacc tggaagataa aaccacccgg tgagaaacag agtgaatgtg
     2701 gcggggtcaa tgacgctgcg gcgcaggttg tccagaatct tccaacgcga cgccagtgtg
     2761 accggattcg gtacggagtt tctgtcataa tccggcacac ggggaaacag ccactgcgca
     2821 atctgccagt cgccgcggac ccaccggtgt tttctgcggc tataggcgct gaagtgggac
     2881 gggtagtcgt cgatcagctc gatgtcggat acaagcgccg aacgcgcata ggcgccctcg
     2941 atcagatcgt ggctcaacag ggcattgcag ggaaaacggt ggtcgaggac cgcgcggagc
     3001 gtgtctacat cgtaaatgcc ctttcccgtg aagatgccct ctccgtagag gtcctgataa
     3061 acgtcggaaa ccgcacgggt atagatgtcg aaaccggttt gtcccgaata aagcgccgcc
     3121 agccgcgaac gcccggccga ctgcacgcta atgccgattc ggggttgcag aatcccgtat
     3181 ccggccacca ctgtgttcgt taccggatcg atgatggcac gattcagcgg gtgcgcgagc
     3241 gtccccacca ttttgtgcgc cgagtcccgc ggaagctgcg tgtcggaatc gagtgtaatg
     3301 acaaactgga tacttggcag aatcgacatg tcgccaactt tcaccggaaa gctgtcgaag
     3361 tcattccgaa gcagcttatt cagatcgagg agtttgccgc gcttgcgctc ccagcccatc
     3421 caggcgcctt cactgggatt gaaaacccgg tggcggtgga agagatagaa acgtccgccg
     3481 ccacccccat atttgcggtt cagcccgcga atcagttcgg agcagaagtt ggccagttct
     3541 tctttctcat cgaacggccg gtccgaatcc ggcgcgtccg tcagcagcgc gtagtagagg
     3601 ttccggtcgg gatttcccag gtagcggatt tccagctcgt tgacagtctc ttccacctgt
     3661 tgttcattca acaacaacgt cggcactgca accatggtgc ggaattcgtc cggtatgccc
     3721 tcggagaagt cgagcttcgg cagcggccgc ggcttgagga gaaaagtggt cagactgttc
     3781 atgaaatcga cggcagcctg cgtagcgggt gtaatcaaca gcaagaaccc ggcgacgatg
     3841 ggggtaaacg tttcgagctg gctcaacaga agaaaagcga tcagcatcgt cgagagctcg
     3901 acgccgatca ggtagaaacc attgggagag ttcagaatgg catcgcgaac ccggcgggca
     3961 aatggcggaa cgtacccgat ttcacgctgc agagcaggca gcccgagatc caccagatag
     4021 aagcctacgt gcgaatggcg ctcgcggatg cgcgttgtgg gagcgtcttc ttcagcggcg
     4081 tgctttgcca gccggacggc cgtggcagcg atatccgttt ccggcgcgcg cgagtgtgta
     4141 gccatctccg cgatggcatt ccggtacata tcgcgcgatt cgtagtccat gcgcgggtag
     4201 gtgagagagg ggtcagagcg caaaatgcgc tccaccaggc tgacctgctc aaacagcgat
     4261 ttccagtcgg tctgggcaac ggcccggagc gaagagatca gccgcccggc cgccggattg
     4321 ccggtttcac cagacgcgga atccgcattg gcgatgcgct cgagcgtagc ggccagctcc
     4381 tgcagcagga cgagttgaag catcggtttt aatgcccaga tttcgcccat ctcgagcggg
     4441 cgctcatcct gaacggcacc aacgtaaaca gccagcgcat cgggacgata gacgaaatcc
     4501 gcgctggtca ggaaactacg cgccagcgca taggcccgcg gtacggatgc tccgccagca
     4561 gaagaaacgt gggggtgctc cgccatgtcg cggtccgggt gcccggcatc gcggagcgcg
     4621 gtgcggatca ggcgatagtt ctcgagcagc caggctcgcg tttcggcgtc gaagggttcg
     4681 cctgtcaatg cgtgcgagga gagcagcgcc cgcaagcgct tatgttccgc ttttagaacc
     4741 ttgccgccgt acctgccggc gtcgacccag gaagaggcag tctcgtgcgc gtgacgccgc
     4801 agcgccgcaa tacgctgcga atcgagattg acggacccga gctcgtcctg aaagtcggcc
     4861 tcgccccgga gcgccgcgga gttggcgtga agcacttcac cacgcacgga aggaatactg
     4921 gaatgcatgt ttcttatcga taactcgcgg cttgtaattc gaacatttcg gaatagcgcc
     4981 caccgagcgc catcaaccgg ctgtgactac cctcttcggt gatgcgcccg ttctcgagca
     5041 caacaatcct gtcggccatg cgcaccgtag agaaccggtg cgaaattaaa agcgccatct
     5101 ttccgtgggt cagttcggcg aatctctgaa aaacctcgtg ctcggagcgc gcatcgagcg
     5161 aggcagtagg ctcgtccaga accaggagct gcgcatcgcg cagataggcg cgagccagcg
     5221 ctactttctg ccactcaccg ccggagaggt ccaaacctcc ttcgaagcgg cggcccagaa
     5281 tctgctcata gccgcgcggc agcctccgaa tcaccgcgtc ggcgagactc ttgcgcgccg
     5341 ccgattcgag cttttccaga tggttccgct cctccacacg gcccatggcg atgttttccg
     5401 cggcgcttac ttcgtaccgc atgaaatcct ggaagatcac gccgatttcg cgggaaaggt
     5461 tctcgatatc gtattcgcgc aggtcgatgc catcgagcag aatctggccg gaaaccggat
     5521 catagagcct cgtcagcagt ttcacaatcg ttgttttgcc ctggccgttc tccccgataa
     5581 gcgcaatccg ctggccgggt tcgatgcgga aatcgaggct gtccagaacc aggcgcgagt
     5641 tccccgggta cgcgaaagac acgttccgga actcgaagcc gtcccgaatc ggacggggag
     5701 cgggtaacgc tccgggcttt gagcgaacct ttggctgcat ggcaaagaac tcgatcagat
     5761 ccgtgaggaa cagggcctgg tctgcgatgc tcgagaaagt ggcaaaaatg ctttggatgt
     5821 tgctactggc cccggcgata gcgcccgcga ggaaagtcat ggtgcccacg ctgagatcgc
     5881 cggtgacggt gcggtaaatc acataggcgt acgctccgta gtaaccggcc gatgtcagaa
     5941 gagagagcat ggaacccgcc cacagccttc gcttcgcgag tgcgacattc tgtttgtaaa
     6001 tgacgtcgga caactcggcg taacgtccgg tgaagaacga gcttaagccg aagagcttca
     6061 gctccttggc cgattccttg ctcgctccca ggacacgaag gtaatccatc tgcctgcgca
     6121 ccggcgtctg gttgaaattc agcgaatatc cgagaaaggc gaagtggctt tcgcccagaa
     6181 aagcgggaac cacacaggcg atgagaatca gcaatagcca gggtgagaat gtgaggatgc
     6241 tgccggccag agtgatcacc acaatggcct gttgcagcat aacgccgaag gcctggatca
     6301 tcgcgatacg gtcggtagcc tgtaccctgg cccgctccag cttgtcgtaa aaaaccggat
     6361 cttcgtaaga ggccagatcg agctgtgagg cgtgttccat cacccggacg ctgacgtgcc
     6421 tggtgaactg gtccgcgagc aggctgtcga aataaccgat ggagcgcccc agaatgccgc
     6481 ccagtgccgc gagcccgaac tcggcggcga ccagccacca gaacaacggg atgagcggtt
     6541 cgccccgggt ggaagcgacc acggaatcga tgatcagccg cgagatggac agcatcgtga
     6601 tgggaatgag tgcggcaatc aggcgggcgg tcaagcctgc cgagacgacg gctggtccag
     6661 actcccaaac aatgcgcagg accggcggta tggtgtaaag cgcgtccagt ctctttttcc
     6721 atccacccca gaacgtttgt gctgagtcaa gcggcataaa cgaaaatctg tctcgtacag
     6781 agcgactaaa accagttgaa cggtgtgctt tagcagtaat actgcagcga ctgcagcaga
     6841 acttgaagta ctctttttga cgcggaaacg gcgaagaagt ttcaaacggc cgggccggcg
     6901 gcggcgcaag tcaattaata ttgaaatagt aaagatagta aatcgcacat ttacgtagcg
     6961 cttgtgcgaa gatggaaatt ccgcagtgca ggatcggggt cccggcgtct ccaggccggt
     7021 ttatagggag tttcagccct ctcggcgagc tctgttgaaa gccgccaccg gtttaagttc
     7081 gctcgcagtt ggcgcgccct tgcgggcgca gacccgcatg gacctgatac cgcatgccca
     7141 gcggtcgagg caggcgctac tcgatgaaat ccacaggcgc tcctgccatt tcttctacga
     7201 ccaggtgaat ccggaactcg gactggttca cgacagggct attcacaccg gacgcgaaca
     7261 gcgggcggtg tccagcatcg cggccaccgg gttcgggctc agcgcgctct gtattgccga
     7321 tcagcagcgg tattttgccg ccggcagtat tcgccggagg gtccaggcga cgctggaata
     7381 cctggcgtcg gaagcgcccc acgaacatgg cttcttcccg cacttcctga atatccggac
     7441 cggcgagcgt atctggggct gcgagtactc ctctgtggac acggcctggc tgctgtgtgg
     7501 tgtactgcat tgccgggcaa ctttcgccga ttccgggatc caggcgctgg ccgatgagat
     7561 catcggccgg gtcgactggc gctggatgtt gaatggcgga ccgactttgt ctcatggctg
     7621 gacgccggag cacggatttc tgccctatcg atgggacagt tattccgaac tcatggccat
     7681 gaacctcctg gcggtgagta cagggactac cactctgccc ggggaaagtt ggcatgcgtg
     7741 ggcacgcccg ttccgaaact ggaaaggtgc ggaatatatc gactctccgg caccgctttt
     7801 cgtgcaccag ttctctcacg cttggttcga tttccgggcg aggcgggacc gttatgcaga
     7861 ctatttcgcg aattcgcgat tggccaccga agcgcaccag gagtactgcg ccggcctgtc
     7921 cgcccagttc ccctggtaca gcatggacat gtggggaatt acagcttccg agtccgagac
     7981 gggctacgtg gcttggggta cccctgccgc gccggttccg cccaatggca ctctggtgcc
     8041 gtgcgcagcc gccggatcga tgccttttct cccggatcag tgcaccaccg tactcgaaac
     8101 catgttggag cgatatggga ataaggtctg gacccggtac gggtttgtgg atgcgtttca
     8161 tccgggaaaa ggctggtaca gccccgacat catcggaatc gacgtgggca tcacgctcct
     8221 gatgattgag aacgcccgca cgggctctgt atggaacgcg atcatgtcga cggtggaagc
     8281 gcccagggct ttcgacatcg tgggcctgca aaaagcttga gaagaaggcg cttgccggcc
     8341 gttggaaggg cggcccggag ccgccgggcc acgtcgagtt tacttgacct tgacccgcaa
     8401 gagacctaga atcgagagta gccccattgt gtgctggaga gtggtatgaa cacacggcga
     8461 ttcttggcgt ttttgagtat cacggctttc ctgacggcaa ccccgctcgc gatggccgcc
     8521 gaaaaaaaag cggccggcgc ggaacgtgaa acgctcgcca aaccaatgag cgagaaggag
     8581 cggcgcaaac agcaggaacg gctccggaaa gagctcgagg gcccctaccg gaagtggctg
     8641 aacgaggacg tcgcctatat catcacggac gaggagcgcg cggcattcaa acgcctctcc
     8701 accgacgatg agatcgaaca gttcatcgaa cagttctggc tgcggcgcga tccctcgccg
     8761 gactccatcg aaaacgagta taaggaagaa cactaccggc gcatagctta tgccaatgag
     8821 cggtttgcgt ccggtatccc cggctggaag tcggaccgcg gccgtatcta tatcactttc
     8881 ggcccgcccg acgagaatga atcccatccg tccggcggtt cctatcaacg ccccatggaa
     8941 gaaggcggcg gcaccacgtc tacgtatccc ttcgagaagt ggcgctatcg ctggatcgag
     9001 ggcatcggca cggacgtcaa catcgaattc gtcgacccga cgatgaccgg ggagtaccgc
     9061 atgacgatgg acccctcgga gaaagacgcc ctgttgatgg ttcccggcgc cgggttgacc
     9121 ctgatggagc agatggggtt ggcatcgaag gcggaccggt tcagccgcac ggacgggacg
     9181 cggttgggtg taggcgatac gcctctgccg tcgcgcatga atcagttcga acgtctggag
     9241 cagtacgcga agttgcagaa accgccgtcg attaagttca aggacctgga agcggcggtc
     9301 agttccacca tccgctacaa cacacttccc atggatgccc ggttggattt catccggatg
     9361 acgaactcca ccgttcagac caacattacg ctgcaatttg acaaaaagga tttgcagttc
     9421 cagcagaagg acggtgtggc gaaggcgatt gtcaatatct acgcccgcat cacttccatg
     9481 acccggcgtc cgatcaatgt gttcgaggat gtcgtgacgg tggaagtccc cagcgaactt
     9541 ctgcagcagg ccagcaaggg ttcgtcggta taccagaaat cgattttcct ttccccgggc
     9601 acgtttcgtt taaacgtggt agcgaaagac gtggttggtg gcaacatgac caactatgag
     9661 accgctatcc atgtgccccg ctatgaagac gagaaactcg gggcgagcac gctgattctc
     9721 gccgatacca tcgaaaaggt gccgactcgc agcatcggga cggggcaatt tgtgatcggg
     9781 acttccaagg tccgcccccg gctgaatgaa acgttccggc gtgacgagaa gatggggatt
     9841 tatctccagc tttacaactt ccagcccgat gaacagaccc agaagccgaa cgcgatcgta
     9901 gagtacgaag tcattcagga tgggacgaat aaaaaggtgt tcggctatac ggaggaagtg
     9961 aaaggcatgg aaggcgcctc cgcgacccag gtgacaatcg agaagttgct tccactccaa
    10021 acgatggaac cgggtaagta caccataaag ctcaaggtga cggataagaa cacggaccag
    10081 gtactaactc ccgaagccac gtttacgata acataggaga tagtgggatt atccccacgt
    10141 tggaggggtg gttcgagctt tctgatgcgt tccagaaagc acatcgtcgc agcggttctg
    10201 gctggatcgc tcttctggcc gggcggcgct gtatttgccg ccgcgcccga caagctatcc
    10261 ggtagccttg tcggtttcgt cagcgatacc gccggcattc cgcagatggg cgctaccgtt
    10321 cttcttttca atcgctccga gcgtcttgta aataaaacac tgaccaacga taagggcgcg
    10381 ttcggcttcg attctctggt tcccgatctc tacagcatcc gcgttactct ggccagtttc
    10441 gttccggcaa tgaaagccgg catcgctata cagcccggcg ccaggagttt tctgtcgatc
    10501 aacctggcga gcgtattgag ttccatcgag ctcgtatata cggctccggg acagggcgcg
    10561 attatgaacg acgactggaa gtgggtgctc cggagctcga tgtcgacgcg gccgatactt
    10621 cgtattgtgc cccaggtcga catttccgac cctagaagca agtcgagcac aaccgctgtg
    10681 ctttcgcgca cacggggcat ggtccggctg tcggcgagcg atatgggcac cgtttccacc
    10741 ggcgcgtcgg aacccgatct gggcaccgcc ttcgcgctcg cgacgtcgtt gatgggcaaa
    10801 agtgagctcc aattcagcgg cggcgttggt tattcgcctg gtgggggcac gccgtccgcc
    10861 ggcttccgga ccagcttcag ccggaacaat gccactggca tggggcccga agtcgcgctg
    10921 acgatgcggc agatgttcct gccggcacgc gccgggctcg cattctttac cggcagatcc
    10981 ggcgcagcgc cggcactgcg aacgatgtcc gtaagcgcat ccgaccggca gaaactcggc
    11041 gattctctgg aactggaata cggtgcatct ctcgaagccg tatcgttcat agagcatctg
    11101 aactatttca gcccttacgc gcgcatgtcg tatgcgcctt ccgatacttc cacgttcctc
    11161 gtcggattca gctcaggtgt gccgcccgcg accggaatgg agtggcccac ggacgacttc
    11221 cagggcgacc tggccgtgct gtccgctttt ccacgggttt ccctgcgggg cggcacggca
    11281 agcgtgcagc gttccgagaa tgtcgagatc ggctatcgaa ggaagtccgg cagccgcacg
    11341 tatgccgtcg gcgtctatcg tgagcgcgct tccaacttgg cattgagtgc cctggcgccg
    11401 gcgggcgtct acggcgcgga tctgctgccc gacctgttct cttccagcgg ctcgattttc
    11461 aacatcggcg actttgagcg gttcggctac gtggtgtctc tcactcagaa tctcggcgac
    11521 aatctaagcg cctcggcggc gtatggcaat cgggaagtgt tggcgccgcc gatgatggat
    11581 tctcccggtg ccggccagtt gcgttcgagt ttcgaccgga ggagaagcgg ttgggtgagc
    11641 gcgaccctgg cgggcacgtc gccgtggacc gggacgcgat ttgttaccag ttatgagtgg
    11701 accgattatg ccatgctggc tccgatgcgc cgcgacctct tgcagcattt gagttcggag
    11761 ccgggttgga atgtacggct gcgacagccc atcccctcga ttccagggat gcgtgggcgc
    11821 ctggaggcta cagccgaatt gcgtaacctg ctggcacagg gctatgtccc cgtgagtgcc
    11881 cagggcaaac ggcttttgct ggttccctcc ccccggtcga tccgcggcgg attgagcttt
    11941 atcttttagc tcacgcagcc gttcacaggt cggaccgggc aactatccaa ctgaaaataa
    12001 gacgatttgt cgcgaacgga ggccggacgt gttggccggg gccgatatcc ttgtgacttg
    12061 agggaatccg gtccagccta aacagcttcc aagcagtcct aaaaccctac cgcacacata
    12121 ctctcccgct cccattgaag agcgtacttt ccccgccgga ttccctcggc tccttttgaa
    12181 catgaaggcg gaccggaaat ggcccgcccg aatcaatcag aatgcaggat gttcctgcgt
    12241 aggatgcgga ttcttccttg cccgcgtcct ggacgtcgtg ccggtagtgc gcttctttgc
    12301 ggtcgatttg gtctgcttcc gccgtgttgt cgtcgttttc ttcggcttgg gtatcggttc
    12361 gagaaacgat ggctgcccct cgacaatagg ttggggttct tcctgctctt cgataggttc
    12421 agcaggttcc gcttcccgtt ccggtagcgc cggcggatag aattcggcgc caagatacac
    12481 catcaaacct ttttcctcgt ccggctctac ccgcaccacg gtcttgtcct gcgcgtaatt
    12541 cagaaattcc gcgaactcct gaaaaccgta agccttcggg tcgaaagaag gatgttccgc
    12601 atgcatccaa gtggcgagat cgtcggcggg ggctccgttt tcgatctcca tccgatgcgt
    12661 ctcgagcaca tcgcgtaagg agggaagagc ctcttccgga tgcagcaggg cgttctccgg
    12721 cacctcggcg ctcgatttct tgcggatggt gtagcgtcct tccggcccgc tgacttcgac
    12781 gaaatccgct ttcttgagct tgtcgacaaa gtcggagaag ctactgcctc cataggcctt
    12841 ttcactgaat gtcgaatcca gttgaagcat cgtggatttc aacagtccca actgtggctg
    12901 tacgccccgg cggtccagca cctggagcgc acgctccagc agcggcatgg cttgagcgat
    12961 atcgagcgga gtgggcctct ggcgcggcga gcgcggatga tgctgttgac gctcctcgct
    13021 tttcacaccg gctccagggc tcttgttatc gtccagcagg gattcgtagc tgatgaactc
    13081 gtggcagttc tgctggagga tggtgctggt gaacgccctt cccccaacca cgtagacggt
    13141 ttttccaaac tctttcagct tattgacgag aggaataaaa tcgctgtctc cgctgacgat
    13201 ggcgaacgca tttacatgac tatgcgtgta cgccatctcc agcgcgtcga gagcgaggtt
    13261 gatatccgcc gcattcttgt ctccacgcgg cgacgggatg cgctggacca tttggacggc
    13321 gttctccgcc atctgcctgg tggcaccttc ctgccgggcc cagttcgcat aagcgaactt
    13381 ggcgatgata tcaccccgct ccttcaacgc ctccagcggt aaggaaacgt caaaccctcg
    13441 ccttagcgtg gattttacgc cgatctcgat gttgtcgtaa tcgacaaaca ccgcaatatt
    13501 cattttttct gccattgagt gttctcttga aggccgcaaa tctcaagcgt tctgtttcac
    13561 ggaggagcga ataaattcga cgatctcccc cagcgacgcg cccggctgaa atactgcggc
    13621 gactccatgc ttttttaatt cctgaacatc ttcatccggt ataatgccgc cgacaacgac
    13681 gatgacgtcc gtcatctcgt gttcgcgaag cagatccatg atccgcggga cgatggcatt
    13741 atgcgctccg gacaggatgg aaaggccgat cacccggacg tcctcctgcg acgccgcgtt
    13801 cacgatcatc tccggtgtct ggcgaagtcc agtataaatc acttccatcc ccgcgtcgcg
    13861 cagcgctcgt gcgatcacct ttgctccgcg gtcatgaccg tctagtcccg gcttcgccac
    13921 cagaacccgg ataggggcat ccattctctg tgtcgacctt tcgaattata aaacgctacg
    13981 ctcccgatag gaaccgaata cgtcccggaa ggcgccgcag atttccccca ccgtcgcgta
    14041 ggctctcacc gcatcgagga tgaacggcat ggtgttggcg ccgtccgcgg cggcccgctt
    14101 cagactgtcg agagccgatt gcacggcagc cgcatttcgc cgggcacgca aacgcgcgag
    14161 tttttcactc tgccgggact gggcgttttc gtccacctga agcagttcga tttcttcgcc
    14221 gccgcccgta aaagcattta caccgacgac gatacgctcc ttcttttcga tggagcattg
    14281 gtatcgatag cttgcgtccg caatctctct ctggggaaaa ccgtgttcga tggccgggat
    14341 catgccgccc atcccgtcga tctgctcgat gtagcggcgg gctgcttttt caacttccaa
    14401 tgtaagcgcc tcaagatagt agctgccgcc gagcgggtcg ggcgtagagg taatgccgct
    14461 ctcatacgcc agtacctgtt gcgtgcgcag cgcgatcgtg acagcctgct cgctgggcag
    14521 cgcgtacgcc tcatcgagag aattcgtatg caacgattgt gctccgccga gtacggcggc
    14581 aagagcctgg atcgtcgtac ggacgacgtt attgtacggc tgttgccagg tgagggaaca
    14641 gccggccgtc tgcgcgtgaa accggagttt catcgtccgc tcgtcgcgtg ccccgaagcg
    14701 gtcgcgcatg atatgcgccc agagtttgcg ggcggcgcga tacttcgcga tctcctcgaa
    14761 aaagtcattg tgcgcattga aaaaaaagct cagccgggga gcgaaatcgt ccacacccaa
    14821 accgcgctcg agcgcccact cgacatattc gatgccgtcc cgcagcgtga acgcaagctc
    14881 ctggaccgcc gtggaaccgg cctcccggat atgatagccg ctgatcgaaa tcggattgaa
    14941 ccggggcaca tatttcgcgg cgtattcgat cacgtcggtc accagccgca tcgatggacg
    15001 gggcgggtag agaaactcct tctgcgcgat gtactccttg agaatgtcat tctggagcgt
    15061 accggagagc gcggaccagg agacgccctg ctgttcggcg acagccagat acatcgccca
    15121 gataaccggc gccggcgagt tgatcgtcat cgaaacggta acggtatcga gcggaatgcc
    15181 gcgaaacagc gtctccatgt cttcgagcga tgagatcgaa acgccgcact tgcccacttc
    15241 tccgagtgac gcggcatcgt cggcgtccag gcccatcagc gtgggaagat cgaaggcaac
    15301 ggaaaggccg ctctgcccct gctccagcag gtataagtaa cgggcgttgg tctcttcggg
    15361 cgtggcaaag cccgagaatt gccgcatggt ccaaagcttg ccgcggtaca tcgattcgtg
    15421 aatgccgcgc gtgtaaggaa actctccggg gcgggcgagg tcgcgggcag cgtcgaagtc
    15481 cttccagtct tcggggccat atacgggcgc aattggaata tcgcttagcg tgcagaaaga
    15541 tcggaccgcc gcgtccggcc cagcgcccag ggacttcgac atcaattcca ttgtagtagc
    15601 gccaggcggc atcgcgccta cccgaccgaa tacaattgtc gagtgtccga gaaaatgttc
    15661 aatgaaccgc gcctggcgct gaaccgtatt tacacgcggc ggggggattc cggcgaaacc
    15721 gcgctcaccg gaggccagcg ggtttcgaaa acctcgccgc gcatcgaatg ttacggcacc
    15781 gtggacgagc tgaacagttt cgtcggaatg gcttgctgct ccatccggaa agcggcgccg
    15841 gcggagcccc gattggcgga gctcgaacgc atcctggtgc gcgttcagca tgagttgttc
    15901 aacctgggct ccatcctctc cacgctgccg gcggacgttc atccgaaaca gccgcgcatc
    15961 accttgcgcg aggtggagca actcgaacag gacatcgacc ggatgaacgc gccgctgccc
    16021 gctctgcgtt cctttgtcct gccgggaggc acccgcgcca atacggaact gcacgcctgc
    16081 cgcaccgttt gccgccgcgc ggaacgcgtg tgtgttcacc ttgcgcagca ggaagaaatt
    16141 ccgccggaat gtattcacta cctgaaccgg ctgagcgacg cctttttcgt ctggagccgg
    16201 tgggtgaact tcgttatggg cgtacctgag ctcctgtggg agcccaatcg cgctgcttcc
    16261 ggtctcgaac cggacagccc gcccgaacca tagcctggaa gagttcctcg ttcgcgcgcc
    16321 ccgaatcctt gaagaagtag tagcgctgtt cgccgcgccc gcggcagagc gtgagctcgt
    16381 ccacaagaat gcgccggcgg aacgcaacct cactgatgct ggaaagcgga atgcgatagt
    16441 gccgctctcc gaaccaccgc cgggcggaaa actccagtgc atcgggatgg agagcaatcg
    16501 acccgatcga atttttcagc cccgccgcac cgttcgccgc tacggctctc aggaaaccgg
    16561 agtccggcag gccggtacgg tccgccgtcc agctccgcaa taaagcggcg agcgccgcca
    16621 gttggaagcg gaccaggcgg a
//
LOCUS       DASTMK010000445         9679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_55368, whole genome shotgun sequence.
ACCESSION   DASTMK010000445 DASTMK010000000
VERSION     DASTMK010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55368"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFQ79_19045"
     CDS             <1..412
                     /locus_tag="VFQ79_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141828.1"
                     /translation="RSGARVATAWPLSDALRRPELGYVSRPLNVVALEDFERDSIGSL
                     RGKPVDAFVLFSLRREPRWSLVPDALTRHILRRYYGFEGPLDAEETARILTLERAASW
                     SHGGEWAEVLLSQNNHANRSLRRCERFTATDGHR"
     gene            complement(489..2144)
                     /locus_tag="VFQ79_19050"
     CDS             complement(489..2144)
                     /locus_tag="VFQ79_19050"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0141829.1"
                     /translation="MKELLSALLITLLAPPVCGAQGGPAAGSNGVVVSGKVAATAAGI
                     EILEQGGNAADAGAATLLALAVTSVGAFCIGGEVPLLIYTAADKNVRVLAGQGGAPRD
                     PKAIEWYLEHGIPGGDIKAAAVPAAADAIVTLLRMYGSRSFAEVVEPTLKILDAGGPT
                     WYIDTGSREKIETGVQWQSDLAATFRKMVDAERRATGSREQRLQAVADRFYRGDIADA
                     LESWYREKGGFLRKADLAAHRTRVEEPVHVNYRGHTVYKCGPWTQGPSMLEMLRLLEG
                     FDLKKMGFLSPDYIHVVAEAMKLGFADRDEYYGDPEFVQVPLQALLSDQYTQIRRPLI
                     DSKKASLELRPGDPYRMKSVKPPFRTETARGGTTTMCVTDRWGNVVVATPSGLSSTAG
                     VAGRTGIIHGARLTSLNNWKGHPNVIAPGKRPRVTLTPTLVLKDGKPVMAIAVAGGDL
                     QEQAALQLVLEYSDFGMDAEKAFRAPRIATHHFIGSFGQDKARLGSLQVPVTLGEEAR
                     QDLQNRGHVLTTGKGSVGGVALLVLDPSSKNVVGIGSSARRVE"
     gene            complement(2154..4274)
                     /locus_tag="VFQ79_19055"
     CDS             complement(2154..4274)
                     /locus_tag="VFQ79_19055"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEU0141830.1"
                     /translation="MSRCIRPIAAAVIALSAAGGLYAQQAWKPNTDWAHWRLGHKTDF
                     KFLESNRLTVTFGSGAPNFEDVTRVQFDAGMEKAKAANRVFHDKSYIVLRYLSSSLNG
                     ESDSGESQPRKDQLRALKFYRENWEAFTGYIGPRPANDPTSWITIRPDGTFPFYRYAP
                     YGQKTGRRFETWGCPNNPDYARLMEGRIRAQAETGIDGSYIDWTQIATNTCYCEFCRT
                     KFAAWLTQHLPKEAAEAKYGTTTYRGVAPPQKRGDPFWMEWITFRGYTVAEFHRRLRQ
                     VAREYNPTFLISGNVYGGFGYGPIAYDAAGNMEMLGREGYDDFIYSEIQEYLDTAPRK
                     NEAGIKITNSPALKFLAAAAHGKPVIIYATEITPPIFPNPTEKCLSAMAQINIAEAAA
                     NHAVFREKRETPPGATAIYNFLANNEANLATAQLTANVAILASINQYLADELSFAFTT
                     SRVLADRGISHVMIVEDDLADPSLNRYGVIVVPYLPLLSTTKQQALVRYVEDGGTLLV
                     LGRSGMKDQYNVPLKSSPLASLVGQKHKAGRVEFVPLPIPTSKYLIPMKARGEYTTFG
                     PTMADLFADIPEGYTRNRIDPALRRILESVADKVTGLGAAGLTRLAVPAPYVEITSMV
                     TARQDRMLIHLVNYNVTIDGEITPAKNIAVNVAPPAAKKVRSIGYSGTLSALAPVKFT
                     PNGKGVRFTADQLGIYGLAVVELE"
     gene            complement(4286..7813)
                     /locus_tag="VFQ79_19060"
     CDS             complement(4286..7813)
                     /locus_tag="VFQ79_19060"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0141831.1"
                     /translation="MLRSITRLAVSFAVVLVLIPAAATGQASFEAQVRGVVHDASGGV
                     LVGAKVTITDIATNISNTATTDERGFYIFNALRPATYTIRAEMAGFRTEETKNVVLAI
                     SQSTNVNFSLRVAGVETSVTVVDAAPTLDTGGAEIGTSVSGQYTREIPLYGRSFFGLV
                     FLSGGITESAGSGNRDSYPSGTNFVSNGQRNSTAEVRFDGAPVSAPEQGEGGNTNVYY
                     TPSVEVIQEFKVENNSFSAEYGNNGGTVVNLVMKQGGNALHGSGWYFGQRDALDANDF
                     YSNVFGIEKPPHVHDQYGGATSGPIKRNKTFFLFDFEKSRSINSWQAAGTFPTSDQRQ
                     GDFSKTMTYDDEGNLAPVTIYDPFSMNEDGIRQPFAGNVIPQGLIDPVAKQFLAYVPT
                     PNVAGEAGTNYNNYRKNVRSSGSAYQFDARVDHQLNDFHRLGVRYSRLSNDSPQDVSF
                     VEDSFTYKTSVHNAVADYTWTVTPAIVYSGRFGVDRVSAPGITHYPDLTSVGFPSYMI
                     NNGLARMPTMAFENAYTNLYDECCVDTNFAHTLYTYSSSLSWVRGSHSIKFGGEQRTF
                     FNNFWQPDNPTGLFTFGPNVTNESPDNGVVTEGDSFAGLLLGWGSADGELRIKPPVAD
                     KSKETGFYIQDDWKVTPKLTVNIGLRYEWSTPYTERYNRSTFADFAAGTGVDVPGLGQ
                     LRGITIFPGPGRRTLPIDRNNFAPRLGFAYSPDQKTVIRAGGGIYYGANMQTNFQYSG
                     PAYFKNAIAYFTKDNYQTQYATLSNPFPNGIAPPQGDKYGKLAMWGFDNGGDLSYELN
                     KGAEIYQWSAGIQRLLPRDIVVAVDYSANRSTHLPWGSFSAGTRNRNFIPSDIRVNYT
                     SSQLNSLVPNPFQPMFVGPDAIFNEPDSRYNDEEIPLLNLLRPYPQFDGSFSGLPLQA
                     AVSRYNSMQIRFDKRNGKYFVIQGSYTLARAMDNSSSGANGWIGWLGSGGPQELDRLG
                     REYTISANNATHRFAAALTANLPIGRGLPVGTNMSRALDAVIGGWSASSSMTLQSGQP
                     VHIEMSRRRLSGGRQRPNLTCSSPGTGVGYKNAAAALLNGDSDGIFVFNSSCFADPGD
                     QQPGNTPRYLEGLTSQGIANVDIGLRKQFSLSEAAKLQVRMEAFNAFNRTRFQRADFG
                     WGSGSFGTVSELAEGFRPRQFQFVVRVEF"
     gene            8081..9625
                     /locus_tag="VFQ79_19065"
     CDS             8081..9625
                     /locus_tag="VFQ79_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141832.1"
                     /translation="MIRRSFFKTAALAGLAVPVSRSPAQVLAHNWDRYDFGSGPPVGG
                     RLNQGPFPQYPPEARFPDSDVVMATTPASEVVPNYGRGLITYIAADMGLDEIVGDDKP
                     KAIEQLVKLPLGQKLYIRPTWREVQPRRGRLEFPEWWKLTFELARQYDKRIGFRIQMR
                     APDYREEALPDFVLEKVPMVKLIGEWRRNQKEGFEEPRYDHPFFQEAFRELNGLLAAE
                     LNGNPQVEFMDTFLYGFWGEGHTWPFRNNPFPDYDTAERTWMRMFETQLEHWTKTPLV
                     TNTQPDFSVVGNAAVLDRSLRTHNWIRTDSIFIENMQIEALSNRPPWVAAVLEVGMHE
                     PRAGSPQAASPAPGQISDMENRIAHVLDLGANYWSLWNFHRISADGVLNSYRKFGKLF
                     DEINHRIGYNVRPSFVWSYGGDNPGLIIGLANDGIAGVPGVLRISVVSENGKISSGGG
                     LDPGYPLPGKIRQAQIPLPKGTKWEGLRLRAELEVKGVRYPVRWACYQKTNPDGSLTL
                     SRNVRG"
BASE COUNT         1861 a         2840 c         2956 g         2022 t
ORIGIN      
        1 tcgttccgga gcgcgcgtgg cgacggcatg gccgctcagc gacgctctgc ggcgtccgga
       61 gctcggctat gtttcaagac cgctcaacgt cgtagcgctg gaagatttcg agcgggattc
      121 catcgggagt ctgcgcggca aaccggtgga tgctttcgtg cttttctccc tgcggcggga
      181 accccggtgg agcctcgtac ccgatgcgct tacgcggcac atcctacgcc gctactacgg
      241 atttgaagga ccgttggacg cggaagagac cgcccgcatt ctgaccctgg aacgagctgc
      301 ctcctggtcg cacggcggtg agtgggccga agtcctgctc tcacagaata accatgccaa
      361 ccgctcactt agaaggtgtg aaagattcac agccacagat ggacacagat aaacacagat
      421 tgaaaaacag gaaacttatc tgtgtttatc cgtgttcatc cgtggctaaa ttcaatttct
      481 tcacagtgct actctacacg tctggccgag gagccgattc cgacgacatt cttcgaggag
      541 ggatcgagaa cgaggagagc gactcctccg acggaaccct taccggtagt caggacgtga
      601 ccgcggtttt ggaggtcctg ccgggcttcc tcgccgaggg tcacgggcac ttgcagactg
      661 cccagtcgcg ccttgtcctg cccgaaagag ccgatgaagt gatgggtggc gatgcgcggc
      721 gcgcgaaagg ccttttcggc atccattccg aagtcggagt actccagcac gagttggagc
      781 gcggcctgtt cctgcagatc gcctccggcg acggcgattg ccatgacggg cttgccgtcc
      841 ttgagcacca gggtgggcgt tagcgtgacg cgaggccgct tcccgggtgc gatcacattc
      901 ggatggcctt tccagttgtt gaggctggtc aggcgagctc cgtggataat gccggtgcgc
      961 ccggcgaccc cggcggtgct cgacagcccg ctcggcgtcg ccacgacgac gttcccccaa
     1021 cggtccgtta cgcacatcgt cgtggtgccg ccccgcgcgg tttccgtgcg gaaaggcggc
     1081 ttgacggatt tcatcctgta aggatctcca ggacgcagtt cgagggacgc tttcttgctg
     1141 tcgatcagcg gccgccggat ctgcgtgtac tgatcggaga gcaacgcctg gagcggcacc
     1201 tgaacgaact cgggatcgcc gtaatactcg tcccggtcgg cgaagcccaa cttcatcgct
     1261 tcggcgacca catgaatata atccggcgac aggaacccca tcttcttcaa atcgaaccct
     1321 tccagcagac ggagcatctc gagcatcgac gggccctgtg tccacgggcc gcatttgtac
     1381 acggtgtgcc ctctgtaatt cacgtgtacg ggctcttcga cccgggttct gtgagccgcc
     1441 aggtccgcct tacggagaaa gccgcctttc tctctgtacc agctctcgag tgcatcggcg
     1501 atgtcgcctc gataaaaacg gtccgcgacg gcctgaaggc gctgttcgcg gctgcctgtg
     1561 gcgcgccgtt ccgcatccac catcttgcga aacgtcgccg ccaggtcaga ctgccattgc
     1621 actccggttt cgatcttctc gcggctgccg gtatcgatgt accacgtggg gcctccggca
     1681 tcgaggatct tcagtgtagg ctccacgacc tctgcaaagc tcctgctgcc atacattctg
     1741 aggagcgtga cgatggcgtc cgccgccgcc ggaaccgccg cggctttgat atcgccacct
     1801 gggatgccgt gctccaggta ccactcgatc gccttcgggt ctcgcggtgc gccgccctgg
     1861 cccgcgagta cgcggacatt cttatcagcg gccgtataaa tcagaagggg cacttcaccg
     1921 ccgatacaaa acgcgcctac cgaagtcacg gccagtgcga ggagggtcgc ggcgccggca
     1981 tcggcagcgt tgccgccttg ttcgagaatc tcaataccag ccgcggtagc ggccactttt
     2041 cccgatacga caaccccgtt gcttcccgca gccggtccgc cttgggcgcc gcagaccggc
     2101 ggcgccagca aagttatcag taaagccgaa aggagctctt tcatcgcccc cgtttactcc
     2161 agctcaacca cggccaggcc atagatcccc aattgatccg cggtaaaccg cacgcccttg
     2221 ccgttcggcg tgaatttcac cggtgcaagc gcggacaacg tgccgctgta gccgatactg
     2281 cggactttct tcgccgccgg cggcgcgacg ttaacggcga tatttttcgc cggcgtgatc
     2341 tcgccgtcga tggtgacgtt gtaatttacc agatggatca gcatgcgatc ctgccgggca
     2401 gtgaccatcg aagtgatctc gacgtagggt gcgggaacgg caaggcgcgt cagacccgct
     2461 gctccgagac cggtcacttt gtctgccacg ctctcgagaa tccggcggag cgccggatcg
     2521 atgcggttgc gtgtgtagcc ctcgggaatg tcggcaaaca ggtctgccat ggtcggaccg
     2581 aaggtcgtgt attcgccgcg cgccttcatc ggaatcaggt atttcgatgt ggggatcggc
     2641 agggggacga actcgacacg gcccgcttta tgcttttgcc caaccagtga ggcaagcggc
     2701 gaacttttca ggggcacgtt gtactggtct ttcatgccgg agcggcccag cacgagcagt
     2761 gtccccccgt cctcgacata gcgcacaagc gcctgctgtt tcgtcgtgct gagcagcggc
     2821 agatagggaa ctacgatcac gccgtacctg ttgaggctgg ggtccgccag gtcgtcctcg
     2881 acgatcatga cgtgggagat tccacggtcc gccaaaacgc gcgaggtagt gaacgcgaag
     2941 cttaactcgt cggcgaggta ttgattgatc gatgccagga tggctacgtt ggccgtgagt
     3001 tgagcggtgg caagatttgc ctcgttgttt gcaagaaagt tatagatagc cgtggcgccg
     3061 ggaggcgtct cccgcttttc gcggaataca gcgtgattcg cggcggcttc ggcaatgttg
     3121 atctgcgcca tcgcgctcag gcatttttcg gtcggattgg ggaagatcgg cggagtgatc
     3181 tcggtggcat agatgatgac gggcttaccg tgggcggccg cggccaggaa ctttaatgcg
     3241 gggctgttgg tgatcttgat gcctgcctcg ttcttgcgcg gggcggtatc gaggtactcc
     3301 tgaatctccg aatagatgaa atcgtcatag ccctcgcggc cgagcatctc catgttgccg
     3361 gcggcatcgt aggcgatggg tccatagccg aatccgccat acacattgcc cgagatcagg
     3421 aaggtcggat tgtattcgcg cgctacctgt ctcaggcgcc ggtggaactc ggcgacagta
     3481 tatccacgga acgtgatcca ttccatccag aacgggtcgc cgcgtttctg tggcggggcc
     3541 acaccgcggt aagtggtagt cccgtatttc gcttcggctg cctccttcgg caggtgctgt
     3601 gtgagccagg cggcgaactt cgtgcggcag aattcgcagt agcaggtatt ggtggcgatc
     3661 tgcgtccagt cgatatagga gccgtcgatc ccggtctccg cctgggcgcg gattctgcct
     3721 tccatcaacc gcgcgtagtc cgggttgttc ggacagcccc aggtctcgaa acggcggccg
     3781 gtcttctggc cgtatggcgc atagcggtag aacggaaacg tgccgtccgg gcggatggtg
     3841 atccaacttg taggatcgtt ggctggccgc ggcccgatgt agccggtgaa cgcctcccag
     3901 ttttcccgat agaatttcag tgcccggagc tggtccttgc gcggttggga ttctcccgaa
     3961 tcgctctcgc cgttcagcga gctcgacagg taacgcagga cgatgtagct cttgtcgtga
     4021 aagacgcggt tcgcggcctt cgctttctcc atcccggcgt cgaactgcac gcgagtgacg
     4081 tcttcgaagt ttggcgcgcc cgatccgaaa gtgaccgtca ggcggttact ttcaaggaac
     4141 ttgaaatcgg ttttgtgccc gagtctccag tgcgcccaat cggtgttcgg cttccatgcc
     4201 tgctgcgcgt aaaggccacc tgctgcggac agtgcgatca ctgcggccgc gatggggcga
     4261 atgcatcggc tcatgaacga tctcctcaga attccacgcg gacaacgaac tggaactgac
     4321 gagggcggaa tccctcagcc agctcactaa ccgtgccgaa tgagccgctg ccccatccga
     4381 agtccgcgcg ttggaagcgc gtgcgattga aagcgttgaa cgcttccatg cgaacctgca
     4441 gcttcgcggc ctccgacaag ctgaactgtt tgcgcagtcc gatatccaca ttcgcgattc
     4501 cctgcgaggt aaggccttcc aggtaccgcg gcgtgttgcc gggctgctgg tcgccgggat
     4561 cggcgaagca cgacgaattg aacacgaaga taccgtcgga gtcgccgtta agcagtgcgg
     4621 ccgcggcatt cttgtagcct actccggtgc ccggactcga gcaggtcaga ttgggccgtt
     4681 gccgcccgcc ggagagtcgg cggcggctca tttcgatgtg gaccggctga cccgattgca
     4741 gggtcatact cgatgaggcg gaccagccgc cgatcaccgc gtcgagcgcc cggctcatat
     4801 tggtgccgac tggaaggccg cggccaatgg gcaaatttgc ggtcagcgcc gccgcaaacc
     4861 tgtgtgtcgc attgttggcg ctgatcgtat actcccggcc aagacggtca agctcctgcg
     4921 ggcctccgct gccgagccat ccgatccagc cgttggcgcc ggaggagctg ttgtccatcg
     4981 cgcgcgccag agtgtagctg ccctggatga cgaagtactt cccgttgcgc ttgtcgaaac
     5041 ggatctgcat cgagttgtaa cgggagaccg cggcttgcaa aggcagcccg ctgaaggagc
     5101 cgtcaaactg aggatacggc cgcagcaggt tgaggagtgg aatctcctcg tcgttatatc
     5161 gcgaatccgg ctcgttgaag atcgcatccg gaccaacgaa cataggctga aacggattcg
     5221 gcaccaggct gttcagttgt gagctggtgt aattcacgcg aatgtccgac gggatgaagt
     5281 tgcggtttct ggtccccgcc gagaagctgc cccacgggag atgcgtactc cggttcgccg
     5341 agtagtccac ggcgaccacg atgtcacggg gaaggaggcg ctggatgccg gcgctccact
     5401 gataaatttc tgcacccttg ttgagctcgt agctcaggtc tcccccgttg tcgaatcccc
     5461 acatggccag cttgccgtac ttgtcgccct gcggcggcgc gattccgttc ggaaacgggt
     5521 tgctcagcgt ggcgtactgt gtctggtagt tatctttcgt gaaatacgcg atggcgttct
     5581 tgaaatacgc cggaccgctg tactgaaagt tggtctgcat gttggcgcca taataaatgc
     5641 cgccccctgc ccgtatgacc gtcttctgat ccggactgta ggcgaatccc aggcgaggtg
     5701 caaagttgtt ccggtcgatc ggaagcgtac ggcggcccgg cccggggaat atggtgatgc
     5761 ccctgagttg ccccaaccct ggaacgtcca ctccggtgcc ggcggcgaaa tccgcgaatg
     5821 tcgagcggtt gtagcgctct gtatagggag tgctccactc gtaccgcaag ccaatattca
     5881 ccgtcagctt cggagtaact ttccagtcat cctgaatgta gaatcccgtt tctttcgact
     5941 tatccgccac gggcggcttg atcctgagct caccgtccgc gcttccccag ccaagcagca
     6001 gacccgcgaa agaatcgcct tcggtgacga cgccgttgtc gggactttcg ttcgtcacat
     6061 tagggccaaa agtgaacaga cccgtcggat tgtccggctg ccagaaattg ttgaaaaacg
     6121 tccgctgctc accgccgaac ttgatggaat gcgagcctct gacccaggac agcgacgacg
     6181 agtaagtgta gagcgtgtgc gcaaagttgg tatctacgca gcactcgtcg tacaaattcg
     6241 tataggcatt ctcgaacgcc atggtgggca ttctggcgag cccgttgttg atcatgtaac
     6301 ttgggaaccc gacgctggtc aggtccggat agtgggtaat tcccggtgcg gaaacgcgat
     6361 ctacaccaaa acgcccggag taaacaatcg ctggtgtcac ggtccaggtg taatcggcaa
     6421 cggcgttatg gacactggtc ttgtaggtaa acgaatcctc cacaaaggaa acgtcctgcg
     6481 gagaatcgtt gctcagcctg ctgtagcgca ctcccagacg gtggaagtcg ttgagttgat
     6541 gatctaccct ggcgtcgaac tggtaggccg aaccgctgga ccgcacgttc ttgcgatagt
     6601 tgttatagtt cgttccggcc tcgccggcga cgttgggcgt gggaacgtag gcgaggaact
     6661 gctttgcgac tggatcgatg agtccctgcg ggatgacatt tcctgcaaac ggctgacgaa
     6721 ttccgtcctc gttcatcgaa aagggatcat agatggtcac cggggcaagg ttcccttcgt
     6781 catcgtaagt catggtcttc gagaagtcgc cctggcgctg atcgctggtc ggaaaggttc
     6841 ccgctgcctg ccacgagttg atactccggg acttttcgaa gtcgaacagg aagaacgtct
     6901 tgttccgttt gatcgggccg gaagtagcgc ctccgtactg atcgtgaacg tgaggcggct
     6961 tttcgatacc gaagacattg ctgtagaagt cgttggcgtc cagcgcatcg cgctggccga
     7021 agtaccagcc gcttccgtgg agcgcattgc cgccttgctt catcaccagg ttcacgacgg
     7081 tgccgccgtt gttgccgtac tccgcggaga aactgttgtt ctctactttg aactcctgga
     7141 tcacttcgac ggacggcgtg tagtacacgt ttgtgttgcc gccctcgccc tgctccggcg
     7201 cgctcaccgg agcaccgtcg aagcggacct cggcggtgga atttcgctgt ccgttcgaga
     7261 caaagttcgt ccccgaagga tagctgtccc ggttaccgct gccggccgat tcggtaatgc
     7321 cgcctgaaag gaacacgagt ccaaaaaagc tgcggccgta gagcgggatc tctctggtgt
     7381 actggccgct gaccgacgtg ccaatctcgg cgccgcccgt gtcgagcgtg ggtgctgcgt
     7441 cgaccaccgt caccgacgtt tccactccgg ccacccggag agagaagtta acgtttgtgc
     7501 tttggctgat agcgagaacg acgttcttgg tttcctccgt ccggaatccg gccatttcgg
     7561 cgcggatggt gtaggtcgcc ggacgcagcg cgttgaaaat atagaaacca cgttcgtcgg
     7621 tggttgcggt gtttgagata ttcgtggcga tatcggtgat tgtcaccttc gcaccgacaa
     7681 gcacgccgcc ggaagcgtcg tgaaccacgc ctctcacctg ggcctcaaag ctggcctgac
     7741 cagttgcggc cgcggggata agaaccaaga ctacggcgaa cgaaactgcc aacctggtaa
     7801 tgctgcgcaa catcgaagcc tcctaagaac atctgagccg ttggcgaagt cagacaattc
     7861 ccgaagctca ctgcgttctc cgaatcggga acgcctcttt cacgcgactg tcgagatagt
     7921 atcgctcgtc tatgggggtg tcaaggcata gtctggcctt gaaattcttg caatccgtgg
     7981 atttgctttg taccatgtac gctagctgag aaccgaaccg ctcatgcccg gcttcgtgct
     8041 tcgcaacggc gggcacggtt aaagcaagag agcaagcgaa gtgatcagaa gatccttctt
     8101 caagactgcc gccctggcag ggttagcggt tccggttagc agatcgcctg cacaagttct
     8161 tgcccacaat tgggaccggt acgatttcgg ctcaggcccc ccggtaggcg gccgcctgaa
     8221 ccaggggcca ttcccacaat acccgccgga agcgcggttc cccgatagcg acgtcgtcat
     8281 ggccacaaca ccggcttcgg aggttgtacc gaactatggc cgggggttga ttacctatat
     8341 cgccgccgac atggggctgg acgagattgt cggcgacgat aagccaaagg cgatcgaaca
     8401 actggtcaag cttccactgg gacaaaaact gtacatccgg cccacctggc gcgaggtaca
     8461 gccccgccgg ggcaggttgg agtttccgga gtggtggaag ctcaccttcg aacttgcgcg
     8521 ccaatacgat aagcgcatcg gatttcgcat tcagatgcgc gcgccggatt accgcgaaga
     8581 ggcgctaccc gatttcgttc ttgagaaggt accgatggtg aagctcatcg gagagtggcg
     8641 gcgcaaccag aaggaagggt tcgaagagcc tcgttacgac cacccgtttt tccaggaggc
     8701 gtttcgcgaa ctgaacgggc tcctcgcggc ggaattgaac ggcaatcccc aagtggagtt
     8761 catggacacg ttcctttacg gcttctgggg agagggccat acctggccgt tccgcaataa
     8821 tccgtttccc gattacgaca cagccgagcg gacatggatg cgcatgttcg agacgcaact
     8881 cgagcactgg acgaaaacgc cgctggtcac caacactcag cccgatttca gcgtagtggg
     8941 caacgcggcg gttctggacc gttcgctaag gactcacaac tggataagaa ccgacagcat
     9001 cttcatcgag aacatgcaaa tcgaagcgct cagcaaccgt cctccgtggg tcgcggcggt
     9061 gctcgaggtc gggatgcatg agccgcgtgc cggttcgcca caggcggcgt cacccgcacc
     9121 agggcagatc agtgacatgg agaaccgcat tgcccacgta ctggaccttg gtgcgaacta
     9181 ctggtctctc tggaatttcc atcggatcag cgcggatgga gtgctgaaca gctaccggaa
     9241 attcggcaag ctgttcgatg agatcaacca tcgcatcgga tacaatgtac ggccttcgtt
     9301 cgtgtggtcg tacgggggcg ataatcccgg tttgatcatc ggcctggcga acgacggcat
     9361 cgcgggcgtg ccaggcgttc tccgtatctc cgtagtaagc gagaatggca aaatttccag
     9421 cggaggcggt ctggatccag gctatccgct gcccggtaag attcgccagg cgcagatccc
     9481 gcttcccaaa ggcaccaaat gggaaggcct gcggcttcgg gcggagcttg aagtgaaagg
     9541 tgtacgctac ccggtgagat gggcttgcta tcagaagaca aatccggatg gttcgctgac
     9601 gctatcgcgc aatgtgcgcg gttagtgccg gccgccgttg tttgcgccgg tctcctaccg
     9661 ctggcgggcg ccggccagg
//
LOCUS       DASTMK010000446         9880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_246905, whole genome shotgun sequence.
ACCESSION   DASTMK010000446 DASTMK010000000
VERSION     DASTMK010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246905"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..2434
                     /gene="lon"
                     /locus_tag="VFQ79_19070"
     CDS             11..2434
                     /gene="lon"
                     /locus_tag="VFQ79_19070"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU0141833.1"
                     /translation="MLTSKEKSDTKRLPMMPIRDVVIFPYMMTPFVVGRESSVRALEE
                     ALAGEKKIFLATQHDASVDEPRPDEIFSVGTIANIVQSLRLPDGNIKVLVEGVERAKV
                     VSVSDDEGFLRAVVRTSHFKVEAGTQLDALIGRVTTLFEQYVKLSQNLNYESMVAAVR
                     VDEPGKLADTVGANLQLTIEEKQELLEIFDPVDRLTRVAEMLDIEIEKLNVDRTIQGR
                     VKRQMEKAQKEYYLNEKIKAIQKELGRGEKSELDELKKKIEAAGMTKDAYDKAITELK
                     RLEGMPPMSAESTVSRNYLDWLLAMPWKKRTKEIRDLQYAQEVLESDHYGLEKIKERI
                     LEFLAVRRLVKNPRGSILCFVGAPGVGKTSLGMSIAKATGRKFVRLSLGGVRDEAEIR
                     GHRRTYIGALPGQILQMMKKAGTRNPVFMLDEIDKMSMDFRGDPSAALLEVLDPEQNF
                     MFMDHYLDVEYDLSQVFFIATANVLHTIPPALQDRMEVIRLSGYTELEKMEIAKRFLV
                     KKQMEQSGLTAQNLQFTDEGLMAIIQSYTREAGVRNLEREVGNVCRKVARSVVNAQSG
                     TDPHTPEVRVVDGKSVAELLGPQKFRDTQSDKKNEVGACTGLAWTEVGGQILTTEATI
                     MEGRGKLTTTGKLGDVMQESAQAAMSYVRSRAQHLGLPRDFYRHLDIHVHVPEGAIPK
                     DGPSAGITIATSICSALTSIPVRCDVAMTGEITLRGRVLPIGGLKEKLLAAHRQGILE
                     AVLPRDNERDLPDIPENIRNEMKLHFVESMDDVLKIALERPIEALPVPAQPAALEATK
                     RAGEEKITH"
     gene            2458..3030
                     /gene="yihA"
                     /locus_tag="VFQ79_19075"
     CDS             2458..3030
                     /gene="yihA"
                     /locus_tag="VFQ79_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEU0141834.1"
                     /translation="MQAEFMCSAARPEDFPPERLPEIAFLGRSNVGKSSVLNRLVGAE
                     RLAFTSSTPGRTQTLNFYRIENRLCFVDLPGYGFARTPKTVSRGWKHLVEAYLSSRKV
                     LELSVLLLDVRRGWMEKDLELRQWLEFHNRRYLVVATKIDKLNHSELQRGLTAIRNVL
                     EGSELVAFSALTGQGVRELWQAIWRNRNQR"
     gene            3186..4457
                     /gene="rho"
                     /locus_tag="VFQ79_19080"
     CDS             3186..4457
                     /gene="rho"
                     /locus_tag="VFQ79_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU0141835.1"
                     /translation="MKNGGAATLDLVELKDMSIQKLNQIAKDLSVPGTAGLRKQELIF
                     KILQTQAEKSGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDVYVSPSQIRRFDLRTG
                     DTISGQIRPPKEGERYFALIKVDAINFEPPEEARNKIFFDNLTPLYPQERLKLETTKE
                     NYSGRVMDLLTPIGKGQRGLIVAPPRTGKTMLLQSLANSITTSHPEVALIVLLIDERP
                     EEVTDMQRSVRGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLA
                     RAYNTVVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIVATALVDTGSRMDD
                     VIFEEFKGTGNMEIHLDRKLVDKRVFPAIDINKSGTRKEELLLPREELNRVWILRKVL
                     NPLSPVESMELLLDKLSKTRSNAEFLAAMSG"
     gene            4546..6399
                     /locus_tag="VFQ79_19085"
     CDS             4546..6399
                     /locus_tag="VFQ79_19085"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEU0141836.1"
                     /translation="MRAISPLTLCFVLAAICAPARGAQLPACDSTPTYSPCEIEFELN
                     AQEAAAHPNPYLTVELQVEFRSPKFRTFRMPAFWDGERRMVVRFTPIDAGAWDYRVSS
                     NVARFEGVMGKFAATDSGSSGFIRPANVHHWATTETNQPHLWMGDTNYRFATMDRKLF
                     EYFVSKRAEQKFNHLRGLVLADPARAYPSPDKPDPAYFREVDERILLMNRSGIVFDLI
                     LGGDENELANLFASREQRERYVRYLVARYSSMNITWQGVQEFEEYENGRDLLRELGEL
                     LKKLDPYGHPRSTHAVVTSSPLLPDGWMTHIIYQSSDNQLGAIEHQIFGVPQVNAEFA
                     YEDSGAGKSHPHHVDTNAFRARLWNSTMNGQYPTFGNTGTYGGKAFPLDAKFLDSPGA
                     KQMTVWYDFFAGTRHWELQPYFDVDGGRAVALEEVEYIVYVEKPSGPVEVLVARHNYD
                     VRWFNPLTGEYVPRKNYKGERFVGEPPDTSHDWVLHISREGRKEGMLRSYKFESRRTL
                     MQEIERNPTRVPFEIVQPSSEEISFSKPPPYSVKLTRETRATRSMMYLWTGEVSTGRQ
                     GARVLGTGVDGTLRSWKALAEQIPGVLNLRVHGMNANGKVYAVDRVYRLAP"
     gene            6396..7073
                     /gene="tsaB"
                     /locus_tag="VFQ79_19090"
     CDS             6396..7073
                     /gene="tsaB"
                     /locus_tag="VFQ79_19090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU0141837.1"
                     /translation="MSARILALDTTSEFGSIALAVGGETIEETLLHSPDGFGHLLFDA
                     ITRALQRHGWAADSVDCFAAASGPGSFTGVRIGLSAVKGLAEALNRPAAAVSNLQAMA
                     LFSESPLRATLLDARRGEIYGAVYDELLAAVVEERVLKFRDWLAILPREEFEFVALDP
                     AFFRPSLAGTRFEQHSWRQAPRALAAAVARIANRKLERGEVTDTAALDANYVRRSDAE
                     LFWKDES"
     gene            complement(7095..8000)
                     /locus_tag="VFQ79_19095"
     CDS             complement(7095..8000)
                     /locus_tag="VFQ79_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEU0141838.1"
                     /translation="MKRMVPVAGLAALAICAAGSLYGQLGMFSKEQRIEFTREWKGER
                     FPDGRPKVPDEVLQRLKTATAEEAWSVLRSERYFNQFEGGWQIVNSGPGRMVGRVVTA
                     VFLPARPDVNAVIDDYGKKEGRVGRGQNSWVIDTLVPGDVLVVDLFGKIKDGTFAGDN
                     LATSIFTKSKNGLVVNGGIRDVSGISEIQGFRAYVRGVDPSAIAGVTMMGINVPIRIR
                     EATVMPGDVVVSDPEGLTFIPAHLAEKVADSSELTQLRDKWGHQMLRSGKYTPGEIDA
                     RWSKEMVAEFNRWAEQQGSKLRVRE"
     gene            8105..8788
                     /locus_tag="VFQ79_19100"
     CDS             8105..8788
                     /locus_tag="VFQ79_19100"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0141839.1"
                     /translation="MRVTGWMIALPFLLPRLWGQAPDPYEALRAAMEASIARQQHSVS
                     GSMEPSVAKQRVSIQAQAQSATPVAGESASTFFTVSWPRPPGFAAEIAPRPECRPVSQ
                     DEIGDAIQEAAEREGLTPDLLRAVIHKESSYLPCAVSPKGALGLMQLMPATASAMKVG
                     NPFDPKQNIDGGSRFLRKLLNRYGNDLSLALGAYNAGPGNVDTYGGLPPFPETIDYVS
                     DILGKLGVQ"
     gene            8899..>9880
                     /locus_tag="VFQ79_19105"
     CDS             8899..>9880
                     /locus_tag="VFQ79_19105"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU0141840.1"
                     /translation="MHITTHESSAINELSSTQPAFLCEGFPHDAPADSCILIVDNQEI
                     NRRVLRGILKSSRYRILEATSAEDALAVLEREKVDLIIVDFVLPQISGPDLCRRLKSN
                     RKTQFIPIVILTSVQGVENEINGMVSGADEFLTQPLHGDVVRTRIRAMLRNKAAVDSL
                     EEAESILFALAQTIEHRDSYTAGHCQRLAAYSSAIGIAMGLPRTSIIALHRGGYLHDI
                     GKIGVPDSILYKQGPLTAEEWEIMRTHTIRGEQICLAMKTLAPVLPIIRHHHEKYDGS
                     GYPDGLRGKEIPLLARILQVSDIYDALTTARPYKPAFSSARAMAILEEECQ"
BASE COUNT         2071 a         2888 c         3039 g         1882 t
ORIGIN      
        1 gtaattttca atgctcacct ccaaagagaa gtccgataca aaacgattgc ccatgatgcc
       61 tatacgggac gtcgtaattt tcccgtatat gatgacgcct ttcgtcgtgg gccgtgaatc
      121 gagcgttcgc gctctcgaag aggcgctggc cggcgaaaag aagatttttc tagccacaca
      181 gcatgatgct tcggtggacg agccgcgtcc cgacgagatt ttcagcgtgg gtacgatcgc
      241 caacattgtc cagagcctgc gtttaccgga tggcaacatc aaggtgctgg tggagggtgt
      301 cgagcgggcc aaagtggttt cggtgagcga cgacgaaggt tttttgcgcg ccgtagtccg
      361 cacgtctcac tttaaagtgg aggcgggcac ccaactggac gcgctgatcg gccgtgtcac
      421 cacgctattc gagcagtacg tcaagctaag ccagaacctc aattacgaaa gcatggtggc
      481 ggcggtgcgg gtggatgagc ccggcaaact cgccgacacc gtgggcgcta accttcagct
      541 tacgatcgag gaaaagcagg agctgcttga gattttcgat ccggtggacc ggctcacacg
      601 ggtggccgag atgctggaca tcgagattga gaagctcaat gtcgatcgca ccatccaggg
      661 gcgcgtgaag cgccagatgg agaaggcgca aaaagagtac tacctgaacg agaagatcaa
      721 ggcgatccag aaagaactgg gacgcggcga gaagagcgag ctcgacgagc tcaagaagaa
      781 gatcgaagcc gcaggcatga ccaaggacgc ctacgataag gcgatcacgg agctgaagcg
      841 gttggaaggc atgcctccca tgtcggccga atccacggtg tccaggaact acctcgactg
      901 gctgctggcg atgccgtgga agaagcgcac caaggagatc cgggatttac agtacgccca
      961 ggaagtgctc gaatccgatc actatggtct cgaaaagatc aaagagcgta ttctggagtt
     1021 tctcgccgta cggcggctgg tgaagaaccc gcgcggctcc atcctctgtt ttgtgggagc
     1081 tccaggcgtc gggaagacct cgctgggaat gtcgattgca aaagccaccg gccggaaatt
     1141 cgtgcggctg tcgctcggcg gcgtgcgcga cgaggcggag atccgcggac accggcgaac
     1201 ctatatcggg gcgctgccgg gccagattct tcagatgatg aagaaggccg gcacgcggaa
     1261 cccggtgttc atgctcgacg agatcgataa gatgtcgatg gacttccggg gcgacccgtc
     1321 cgcggctctt ctcgaggtgc tcgatcccga gcagaacttc atgttcatgg atcactacct
     1381 ggacgtggag tacgatctga gccaggtgtt cttcatcgcg acggctaatg tgctgcacac
     1441 gattccgccc gcgttgcagg acaggatgga agtgatccgt ctttccgggt acacggaact
     1501 cgaaaagatg gagattgcca agcgcttcct ggtgaagaaa cagatggagc agagcgggct
     1561 gaccgcgcag aacctccaat tcactgacga agggttgatg gcgatcatcc aatcctacac
     1621 gcgggaagcc ggagtgcgga acctggaacg ggaagtgggc aacgtctgcc gtaaggtggc
     1681 gcgaagtgtc gtcaacgcgc aaagcggaac ggacccgcat acgcccgaag taagggtagt
     1741 ggacgggaag tccgtcgccg aattgctggg tccgcagaaa ttccgcgaca cgcagagcga
     1801 taaaaagaat gaggtcggcg cgtgtaccgg tctggcgtgg accgaagtcg gcggccagat
     1861 tctcacaacg gaagcgacga tcatggaggg ccggggtaag ctgactacca cgggtaagtt
     1921 gggcgacgtg atgcaggagt ccgcgcaggc ggccatgagc tatgttcgtt cgcgtgcgca
     1981 gcatctcggg ctgccccggg atttttaccg ccacctcgac attcacgtgc acgttccgga
     2041 aggtgctatt ccgaaggacg gtccgtcggc gggtatcacg attgccacca gtatttgcag
     2101 tgcccttacg agcatccccg tacgctgcga tgtggcgatg acgggggaga tcacgctgcg
     2161 cggccgggta ttgccgatcg gcgggctcaa ggaaaaactg ctcgccgcgc atcggcaggg
     2221 catcctggag gcggtgctgc cacgggacaa cgagagggac ctgccggata ttcccgagaa
     2281 tattcgtaac gaaatgaaac tgcacttcgt ggagtccatg gacgatgtgt tgaagatcgc
     2341 cctggagcgg cccatcgagg cgttgccggt tcccgcgcag ccggccgcgc tcgaagccac
     2401 gaagcgagcc ggcgaagaga agattacgca ttaagtttcc ggtccggcca gcgcgttatg
     2461 caggcggagt tcatgtgcag cgcggcgcgg ccggaggact tcccgcccga gaggctgccc
     2521 gaaatcgcgt tcctcgggag aagcaatgta ggcaagtcca gcgtgctgaa ccgcctggtg
     2581 ggtgcggaga ggcttgcgtt cacaagttca acgccgggcc gtacacagac gctgaatttt
     2641 tacagaattg agaaccggct gtgcttcgtc gatcttcccg gttacggatt cgcacggacc
     2701 cccaagaccg tatcccgggg ttggaagcac ctcgtcgagg cttatctcag cagccggaaa
     2761 gtcctggagc tttcggtgct cctgctggat gtccggcggg gctggatgga aaaggatctg
     2821 gaactcaggc agtggctcga gttccacaac cggcgatacc ttgtggtcgc cacgaagata
     2881 gacaaactaa accattccga actacagcgc ggcttgaccg ccatccgcaa cgtgcttgag
     2941 ggtagcgaac ttgtggcgtt ctcggcctta accggccagg gagtgaggga actttggcaa
     3001 gcgatttgga gaaacaggaa tcaacggtag ccgctaatcc tccggaaacg gagaaggcgc
     3061 ccgagcagaa gcccgccgaa caggcggcgc ctgcgccgca gggtcagggg cagggccaga
     3121 atcaggccca gcagcccggc ccgcagaaac gaggacgcgg ccacggtaaa ggcgacgttg
     3181 gcggcgtgaa gaacggaggc gccgcaaccc tcgaccttgt cgaactgaag gacatgagca
     3241 ttcagaagct caatcagatc gccaaggact tgagcgttcc gggaaccgcc ggccttcgca
     3301 agcaggagct gattttcaag atcctgcaaa cgcaggccga gaaaagcggg ctcatctttt
     3361 ccgaaggagt gctcgaatgt cttccggacg gattcggttt tctacgcgca ccggagtaca
     3421 actatctccc cggccccgat gacgtgtacg tttccccgtc ccagatccgg cggttcgacc
     3481 tgcgcacggg cgacaccatc tccggccaga tccggccgcc caaggaaggc gaacgctatt
     3541 tcgcgctgat caaggtcgac gccatcaact tcgagccgcc ggaggaggcg cgcaacaaga
     3601 tcttcttcga caatctcacg cccctgtacc cgcaggagcg gctcaagctg gaaacgacga
     3661 aggagaacta ctccgggcgg gtgatggatt tgcttacccc gatcggaaaa gggcagcgcg
     3721 gtctgatcgt cgcgccgccc cgtaccggca agacgatgct gctgcaaagt ctggcgaact
     3781 ccatcaccac cagccatccc gaggttgcgc tgatcgtgct gctgatcgac gagcggccgg
     3841 aagaagttac cgacatgcag cgctcggtgc ggggcgaagt catcagctcc acgttcgatg
     3901 aacccgcctc gcggcacgtg caggtcgccg aaatggtgat cgagaaagcg aaacggttgg
     3961 tggagcacaa acgcgacgtg gtcattctgc tcgactccat tacgcgtctc gcgcgagcct
     4021 acaacaccgt ggtgccgcca tccggcaagg tgctctccgg cggtgtggat tcgaacgcct
     4081 tgcagcggcc gaagcgcttc tttggcgcgg cccgcaacat tgaagaaggc ggttcgctga
     4141 cgatcgtggc caccgccctc gtcgatacgg gcagccgcat ggacgacgtc atcttcgaag
     4201 agttcaaagg caccggcaac atggagatcc acctcgaccg caagctggtg gacaaacgtg
     4261 tattcccggc catcgatatc aacaagagcg gaacgcgaaa agaagagttg ctgctgccgc
     4321 gcgaagaact caaccgcgtg tggatccttc gtaaagtgct aaatccgctg tcgccggtcg
     4381 agtcgatgga actgctactc gataaacttt cgaaaacccg cagtaacgcc gagttcctgg
     4441 ctgcgatgag cggataggca cgggatacca atcgactcag ccggcggatc tccgggcgcc
     4501 agaacccgtt cgcgggcggc tgctctgccg ctaaactgat ctcttatgcg ggcgatctcc
     4561 cccttgacgc tttgtttcgt gctggccgct atctgcgcgc cggcacgcgg cgcacagttg
     4621 ccggcatgcg attcaacgcc cacgtactcg ccctgcgaga tcgaatttga gctgaacgca
     4681 caggaagccg ccgcccatcc aaacccttat ctgacggtgg agcttcaggt ggagttccga
     4741 tcgccgaagt tccggacgtt ccgcatgccc gcgttctggg atggggagcg gcggatggtg
     4801 gtgcgcttca cgcccatcga tgccggggca tgggattatc gtgtttcaag caacgtcgcg
     4861 cggttcgaag gcgttatggg gaagtttgcc gccacggatt ccggctcgtc cggattcatc
     4921 cgcccggcca acgtccatca ctgggcgacg acggaaacca accagcctca cctgtggatg
     4981 ggggatacga attaccggtt tgcgacgatg gatcgcaagc tcttcgagta cttcgtgtcg
     5041 aagcgtgccg aacagaagtt caatcatctg cgcggccttg tgctggccga ccccgcgcgc
     5101 gcctatcctt cgccggacaa accagacccg gcctacttcc gcgaagtgga cgaacgcatc
     5161 ctcctgatga acaggagcgg catcgtcttc gacctgatcc tcggcggcga cgagaatgag
     5221 ctggcaaatc tcttcgcctc ccgcgagcag cgcgagcgct acgtccgcta cctggtagcg
     5281 cgctattcct ccatgaacat cacctggcag ggcgtacagg agttcgaaga gtatgagaac
     5341 gggcgggacc tgctgcgcga actcggcgag ctgctgaaga aactggatcc ctacgggcac
     5401 ccccgctcga cgcacgccgt agtgacgtct tcgccgctgc tgccggatgg ctggatgacc
     5461 catatcatct accagtcctc ggataatcaa ctgggcgcaa tcgaacacca gatcttcggt
     5521 gtgccgcagg tgaacgcgga gtttgcctac gaagacagcg gggccggcaa gtctcacccg
     5581 catcatgtgg atacgaacgc gttccgcgcc cgtctgtgga attccaccat gaacggacaa
     5641 tatcccacct tcggcaatac gggaacttat gggggaaagg cattcccgct ggatgcgaag
     5701 tttctggatt cgccgggcgc caagcagatg accgtctggt acgacttctt cgcgggcacc
     5761 cgccactggg agctgcaacc ctacttcgat gtggatggcg gccgcgcggt cgcgctggag
     5821 gaagtggaat acatcgtcta tgtggagaag ccgtccggcc cggtggaggt gctggtggcg
     5881 cggcacaact acgatgtccg atggttcaat cccctcaccg gcgagtacgt cccgcggaag
     5941 aactataaag gcgaacggtt cgtcggcgaa ccgccggata ccagtcacga ttgggtgctg
     6001 catatttcga gggaaggccg gaaggaaggg atgctgcgct catacaaatt cgagtcgcgc
     6061 cgcacgttga tgcaggaaat cgaacggaat cccacgcgcg tgccgtttga gatcgtacag
     6121 ccgtcttcgg aggagatttc cttctcgaaa ccgccgccgt actcggtcaa gctgacgcgg
     6181 gagacgcggg ccacgcgttc catgatgtat ctctggacag gtgaggtttc caccggccgt
     6241 cagggcgcgc gcgtattggg cacgggcgta gacgggacgt tgcgaagctg gaaggccctg
     6301 gccgaacaga ttcccggggt gctgaacctc cgcgtacacg ggatgaatgc caacggaaag
     6361 gtttatgcgg tggaccgggt ctacaggctg gcgccgtgag cgcccggatt ctggcgcttg
     6421 ataccacctc ggagttcggc agcatcgcac tggccgtggg tggagaaacg atcgaagaaa
     6481 cccttcttca ttcgcccgac ggattcggac atcttctctt tgacgcgatc acgcgcgcgc
     6541 ttcaacggca cggatgggcg gcggactccg tcgattgctt tgcggccgcc tcgggcccgg
     6601 ggtccttcac cggggtgcgt atcggattat cggcggtgaa aggtctggcc gaagcgttga
     6661 acaggccggc ggcggccgta tcgaatctcc aggcgatggc tttgttctcg gagtcaccgc
     6721 tgcgcgccac tctgctggac gcacgccggg gcgagatcta cggcgcggtc tacgacgaat
     6781 tgctggctgc cgtggtggaa gagcgcgtcc tgaaatttcg cgactggctc gcgattctcc
     6841 cgcgggaaga gttcgaattt gtcgcgctcg accccgcttt cttccgcccc tcgcttgcgg
     6901 gaacgcgctt cgaacaacac tcctggcggc aagcaccccg ggcgctcgcg gcagccgtcg
     6961 cgcgtatcgc caaccggaag ctcgaacggg gcgaagttac cgatacggcg gcgctcgatg
     7021 ccaactacgt gcggcggtcg gacgcggaac tgttctggaa agacgaaagc tgaaagcgga
     7081 ggcttccgcg cgcctcattc ccgcacgcgg agcttcgagc cctgctgttc cgcccagcga
     7141 ttgaactcgg ccaccatttc cttcgaccag cgcgcatcga tctcgcccgg cgtatacttg
     7201 ccggaacgaa gcatctggtg tccccatttg tcccgcagtt gcgtcaattc ggaggaatcc
     7261 gcgaccttct ccgccaggtg tgccggaatg aacgtcagcc cttcgggatc gcttaccacc
     7321 acgtcacccg gcatcacggt cgcctctcta atgcgaatgg gtacgttaat ccccatcatc
     7381 gtcacgccgg cgatggcgga tggatcgacg ccgcggacat aagcacggaa accctgaatt
     7441 tccgagatgc cggagacatc gcgaatgcct ccgttcacca ccagtccgtt cttggatttg
     7501 gtgaagatgg aggtggccag attatcgccg gcgaaggtgc catctttgat tttcccgaac
     7561 aggtccacca ccaggacgtc accgggcacg agcgtatcga tgacccagga gttctgtccc
     7621 cttcccacgc ggccctcctt ctttccatag tcgtcgatga ccgcattcac gtcggggcgc
     7681 gccggcagga agaccgccgt cacgacgcgg cccaccatgc gtccggggcc ggagtttact
     7741 atctgccagc cgccttcgaa ctggttgaag tagcgctcgc tccgcagcac gctccatgct
     7801 tcttcggcag tcgcggtttt caggcgctgg aggacctcgt cggggacttt cggacggccg
     7861 tcggggaagc gctcgccttt ccattcgcgc gtaaactcga tgcgctgctc tttcgagaac
     7921 attcccaact ggccgtagag cgatccagcg gcgcagattg caagagcggc caggccggca
     7981 actggaacca tccgtttcat ttgtaaattc tccctatccc gataccgctt gcttgcgcgc
     8041 gcggctcggt tattagctta agacaaaacc tatagtttat gtcgggcttc gccgatctaa
     8101 ggatgtgcgt gttacagggt ggatgattgc gctgccgttt ctgttgccgc gattgtgggg
     8161 gcaggcgccg gatccgtatg aagcgctgag agcggcgatg gaggcgtcca tcgccaggca
     8221 gcagcactcg gtttccggga gcatggagcc atcggtggcg aagcagcgtg tgtcgattca
     8281 ggcgcaggcg caatccgcga ctcccgtggc gggcgagagc gcatctacat ttttcactgt
     8341 ttcctggccg cgtccccccg gattcgcggc ggaaatagcg ccgaggcccg agtgccggcc
     8401 cgtgtcgcag gatgagatcg gcgacgccat ccaagaggcc gccgaacgcg aggggctgac
     8461 gcccgatctt ctgcgcgccg taatccacaa ggagtcgtcc tatctgccgt gcgcggtctc
     8521 tccaaaggga gccctcggcc tgatgcaact catgccggct accgcctctg cgatgaaggt
     8581 cggcaatccc ttcgatccca aacaaaacat tgacggtggt tcgcgttttt tacggaaatt
     8641 gctgaaccgc tacggcaacg atctctctct ggctctcggt gcgtacaatg ccgggccggg
     8701 caatgtggat acttatggag ggctgccgcc gtttcccgaa accatcgatt atgtcagcga
     8761 tatcctgggc aagctcggtg tgcaatagta taaaagccta ccttggtcta aaattctggc
     8821 tctttagtac taaagaaatc tgtaccattg ccgataggaa acactgacgg cggttttttg
     8881 tcagttcgtc cgtgcagtat gcacataaca acgcacgaat ctagcgctat taacgagctt
     8941 tcttcaacgc agcccgcatt cctgtgtgaa ggatttccgc acgacgcgcc ggcggattcg
     9001 tgcattctga tcgtcgataa tcaggaaatc aaccggcgcg tactgcgcgg tattctcaaa
     9061 tcctcgcgct accggattct ggaggcaact tcggcggaag atgccttggc agtgttggaa
     9121 cgtgaaaagg tcgatctgat catagtcgat ttcgtgcttc cgcagatcag cggacccgac
     9181 ctctgccgcc ggttaaaaag caatcgcaag acacagttca ttcccatcgt cattctgacc
     9241 agcgtgcagg gcgttgaaaa cgaaatcaac gggatggtgt cgggcgccga cgaatttctt
     9301 acgcagccgc ttcacggcga tgtcgtccgg acgcgtatcc gggccatgct gcgcaacaag
     9361 gcggcggtcg attcactcga agaagccgaa agcattctgt ttgcgcttgc ccagaccatt
     9421 gagcatcgcg actcttacac cgccggacat tgccagcggc tggccgcata cagctcggca
     9481 atcggcatag cgatgggact cccccgcact tcaatcatcg cgttgcatcg agggggctat
     9541 ctccatgaca ttggaaaaat cggggtaccc gattccattc tctacaagca aggtcccctg
     9601 acggccgagg aatgggagat catgcgcacg cacacgattc gcggcgagca gatctgcctg
     9661 gcaatgaaaa cgctggcgcc cgtattgccg atcatccggc atcatcacga aaagtacgat
     9721 ggatcgggat atccggacgg gttgcggggc aaagaaatac cgctgctggc ccgaattctt
     9781 caggtgagcg acatttacga tgcgctcaca accgcacggc cctacaaacc cgcgttttct
     9841 tccgcacgcg ccatggcgat cctcgaagag gaatgccagg
//
LOCUS       DASTMK010000447         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_279318, whole genome shotgun sequence.
ACCESSION   DASTMK010000447 DASTMK010000000
VERSION     DASTMK010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279318"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1960)
                     /locus_tag="VFQ79_19110"
     CDS             complement(11..1960)
                     /locus_tag="VFQ79_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141841.1"
                     /translation="MLDMRKGVFLALLIAASAVAQTQPGDVTRYLSTPILPPDVAESE
                     LRTYLMRKVQVPPWPARAENWTAEARQLKERILKQIVFHGWPREWVDGPLKFEDLGVI
                     EANPEYRMRRLRYEIVPGMQSTAILYEPANMQGKVPAILNVNGHVGPLGKSIEYKQKR
                     CINQARRGILALNLEWLSFGELAHSENVHWFAAHLDLVGSNSLGLFYLAMRRGLDYLY
                     QHPSVDRSRIGMTGLSGGGWQTIVLSALDERVFAAVPVAGYSSLASRIERPADVGDVE
                     QNATDLLTIADYPLLTAIRAPRPTLLVYNVEDDCCFRAPLVKPYIFDFVRPYFKLYGD
                     ADRLSWHENTDPSTHNYQLDNRQQSYRFFARHFGMPAAEREIPVDAEIKSYDELAVGL
                     PKDNLTILGVAKVLAARIERRGGPSGNASRSAWAQSQRDLLRQTVRYRPVDLRHAWAV
                     GNSHRRGLETRSYRFEFDNGLSASAVWLQALSASQEGPATIMLNDDGRKAAGGEVSDR
                     VNRGERVLALDPLFIGDAQPRATGLRTFGQLLAATGDRALGIEAAQVASTGRWIRKSG
                     GAKMLRIETSGIRSQVIALVAAVLEPDLFSEVVFRKGMKSLAHLLDAPVEFSNSADLF
                     CLDLYRHFDIEPLTQAATKTRIVWR"
     gene            complement(1962..>3377)
                     /locus_tag="VFQ79_19115"
     CDS             complement(1962..>3377)
                     /locus_tag="VFQ79_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141842.1"
                     /translation="GAKGLHLYPLRYWDWPVTADVVSPPLLQIDRDWIWFEAWARYAW
                     NPKRDAAAERRHWVDRIALRYGSRAAAARILDAYELSGEVLPRLLPRIGITTGARQTF
                     ALGMLMTQLISPARYSPWVEMWEATAPPGERLEEWAKREWEKQPHTGETPPRLADELV
                     QVATRAVADIEAAAPLVTSGHEEFARLRNDVHAIRAIARYYAWKVHAAASVLRYRYSG
                     ELADLDRAQELLDWSLEEYRELVRLTGTAYRDANSLHAASRRIPFISGPGRYIHWRQC
                     LPEYEKEAAVFRRNRARLRDPGRTPPLPTNGASDTRASAVSVKTLTFGAEVFTVGRGA
                     ALFTDRSYTAAEVAHELRGLTGIRISAAAAENGVTVDLEIAEPARILVGFPSGAGHWA
                     TPPKDEWDLVLESGVLAPRLPPVRVFSHLLPAGRNQVNFGKGGFVILGFVKPGSGVEA
                     GLQGRRLSRNRGDLDWLFEEE"
BASE COUNT          612 a         1077 c         1064 g          624 t
ORIGIN      
        1 gcatccccgt tcatctccac acaatgcggg tcttcgtggc ggcttgtgtg aggggttcga
       61 tatcgaagtg cctgtaaagg tcgaggcaaa acaagtccgc gctattcgaa aattcgactg
      121 gcgcatccag cagatgtgcc aaggacttca tccccttacg gaagacaacc tctgagaaga
      181 ggtccggctc aagcacggcg gcgacaaggg cgatgacctg gctgcgaatg ccggaggtct
      241 cgatgcgcag catcttcgcg ccccccgact tcctgatcca ccgcccggtc gaggcgactt
      301 gcgcagcctc gatcccgaga gcccggtcgc ctgtcgctgc cagcaactgc ccaaaggtgc
      361 gaaggccggt ggcccggggt tgtgcgtccc caataaatag aggatcgaga gccagcaccc
      421 gctctccgcg attcacgcgg tcggatacct ccccaccggc ggctttcctc ccgtcgtcgt
      481 tcagcataat tgtcgcaggc ccttcctgtg atgccgaaag cgcctggagc catacggcgc
      541 tggcgctcaa gccattgtcg aactcgaagc ggtatgaccg ggtctcgagt ccgcgtcgat
      601 ggctgtttcc gaccgcccag gcatggcgca ggtccacggg cctgtagcga acggtctgac
      661 gcaacaaatc ccgttggctt tgcgcccatg cactccgcga cgcgttgccg gaaggtcccc
      721 cgcgccgctc gatccgcgcg gcgagcactt tggctacgcc gaggatcgtg agattatcct
      781 tcggcagccc tactgcgagt tcgtcatagc ttttaatttc ggcgtccacc gggatttccc
      841 gctcagcggc cggcattccg aagtgcctgg caaagaagcg gtaggactgc tgccggttat
      901 ccagttggta gttatgggtg gagggatccg tattttcgtg ccaggagagc cggtcggcat
      961 caccatacag tttgaagtac ggccgcacaa aatcgaagat gtagggcttg acgagcggtg
     1021 cacggaaaca gcagtcgtcc tccacgttgt agaccagcaa cgtggggcgc ggtgcccgta
     1081 tcgctgtcaa cagagggtaa tccgcgatgg tgagaaggtc ggttgcgttc tgttcaacgt
     1141 ccccgacgtc ggccggtctc tcgatacgag aggccaggga cgagtagccg gccacgggca
     1201 ctgctgcgaa gacccgctca tcaagagcgc tgagcacgat ggtctgccaa cctccccctg
     1261 aaagtcccgt catgccgatg cggcttcgat cgacagaagg gtgctggtag aggtagtcca
     1321 gcccccggcg catcgcaaga tagaacaggc cgagggagtt ggaccccacc aggtccagat
     1381 gtgcggcgaa ccagtgaacg ttctccgaat gggcgagctc gccgaaggac agccactcca
     1441 ggttgagcgc caggatgcct cttctcgctt ggttaatgca acgcttttgt ttgtattcga
     1501 tgctctttcc caacggcccg acgtggccgt tcacgttgag gatggccggc acttttcctt
     1561 gcatgttcgc cggctcgtac agaatggcgg tggactgcat cccgggtacg atctcgtaac
     1621 gcaatcggcg catccggtac tccgggttcg cctcgatcac gccgaggtcc tcaaacttga
     1681 gcggtccatc gacccattca cgtggccagc cgtgaaaaac gatctgtttt aggatgcgtt
     1741 ccttcagctg ccgagcttca gcagtccaat tctcagcccg ggcgggccag ggagggacct
     1801 ggaccttgcg catcaggtac gtgcgcagct ccgattccgc cacgtccgga ggcagaatag
     1861 gggttgagag atagcgcgtc acatctccgg gttgcgtttg ggcaacggcg gatgcggcga
     1921 ttaacagagc gaggaatacc cccttccgca tgtcaagcat gttactcctc ctcgaacaac
     1981 cagtcgagat ctcccctatt gcgcgaaagg cggcggccct gaaggccggc ctccacaccg
     2041 ctgccgggtt tcacgaaacc gaggatgacg aagccaccct tgccgaagtt cacctggttg
     2101 cgaccggcgg gaagcaggtg gctgaagaca cgcaccggcg gaagccgggg cgccagcacg
     2161 ccgctttcga gcaccagatc ccactcgtct tttggaggtg tggcccagtg ccctgctccc
     2221 gaggggaagc cgaccaggat gcgcgcgggt tcggctattt ccaaatccac cgtcacgccg
     2281 ttctcagccg ctgctgccga gatccggatt ccggtgaggc cgcgcagttc atgcgcgacc
     2341 tccgcagccg tgtaagagcg gtccgtgaac agcgcggctc cccggccgac cgtgaacacc
     2401 tctgcaccaa acgtgagagt tttaacactc accgccgaag cgcgagtgtc tgacgcgccg
     2461 ttcgttggca gcgggggggt gcggcccggg tcgcgcagcc gcgcgcggtt ccggcggaac
     2521 acggcagcct ctttctcgta ctcaggcaga cactgccgcc agtggatgta tcggccgggg
     2581 ccggagatga agggaatccg cctcgatgcc gcgtgcaggc tattggcatc ccggtatgca
     2641 gtcccggtga ggcgcaccag ttcgcggtat tcctccaagc tccagtccag gagttcctgc
     2701 gcgcgatcga gatcggccaa ctctccgctg tagcggtacc ggagaacaga cgcggcggcg
     2761 tgcaccttcc acgcatagta tctggcgatc gcgcggatgg catggacgtc gttccggagg
     2821 cgggcgaact cctcgtggcc ggacgtaacc agcggcgcag cggcctcgat gtccgccact
     2881 gctcgcgtgg cgacctgcac gagttcgtcg gccagacgcg gcggggtctc tcccgtgtga
     2941 ggctgctttt cccactcgcg ctttgcccat tcctcgagtc gctccccggg cggcgcggtc
     3001 gcctcccaca tctcgaccca tggggagtag cgcgccgggc tgatcaactg cgtcataagc
     3061 atgcccagcg caaaggtctg gcgggcgccg gtcgtgattc cgatgcgggg cagcagccgc
     3121 ggcaagacct ctccggagag ctcgtaagcg tcgaggatgc gcgcggcggc cgcgcgtgag
     3181 ccgtatcgta gggcgatccg gtccacccag tgccgccgtt cggccgccgc gtcacgcttc
     3241 ggattccagg catagcgtgc ccaagcctcg aaccagatcc agtcgcggtc gatttgcagc
     3301 agcggcgggg agaccacgtc ggccgtgacc ggccaatccc agtaccgcag cggatatagg
     3361 tgcaggccct tggctcc
//
LOCUS       DASTMK010000448         1746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_311432, whole genome shotgun sequence.
ACCESSION   DASTMK010000448 DASTMK010000000
VERSION     DASTMK010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311432"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFQ79_19120"
     CDS             complement(<1..446)
                     /locus_tag="VFQ79_19120"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0141843.1"
                     /translation="MSRRLRYQVAVSLDGFIAGPNGEYDWIVMDPAIDFTALFKEFDT
                     AVMGRKTYEVLSEQGGYGMPDLDVFVFSRTLPPAMRPGVRIVNDNPREFVAALKAKPG
                     RDIWLYGGGKLFRLLLDGGLVDTVEVAVIPVLLGSGIPLLAPGAGT"
     gene            504..>1746
                     /locus_tag="VFQ79_19125"
     CDS             504..>1746
                     /locus_tag="VFQ79_19125"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEU0141844.1"
                     /translation="MQAGRISWVIVLCGALIPIASAQSNSTPPGVRSFFVQHCFACHS
                     NKAKTAGLDLESPVTDHTVWEDVLRKIRSGEMPPAPMPRPRPADIAAVSKWIASVPLR
                     PGTQPQKVDPGRVTARRLNRAEYDNTIRDLLAVDYRPARDFPADDSGYGFDNIADVLS
                     LSPVLMEKYFAAAEEISRIVLPGNVAPKPTAERYKQEQTAGAKPGFLEIRHRFPAEAE
                     YDLRVVLGGVRPPRSPEIRMAFLLDGRELQTFGVDPGRGKPRSFGTRLVVKAGERNLR
                     AVFLNDDYNPEEHSLNATDRHLTIDYIEVRGPYNIRGTLTESQQKVFVCGHVADHHHP
                     DCVRDNLSDLARRAFRRPVSKEETGPLVRFVKLAAEQGDTLEDGMRLALQAILVSPQF
                     LFRIERDPPGGAAHPVTDFELA"
BASE COUNT          353 a          552 c          496 g          345 t
ORIGIN      
        1 ttcgttccgg cacccggggc aaggagcgga atcccacttc caagcagcac aggaatgacg
       61 gcaacttcca ccgtgtccac aaggcccccg tcgaggagca agcgaaagag cttaccccca
      121 ccgtagagcc agatatcgcg gcccggcttc gccttcaggg ccgccacaaa ctcacgcgga
      181 ttatcgttta caatgcggac gcccgggcgc atagccggag gaagtgttcg tgagaacacg
      241 aaaacgtcca gatctggcat gccgtaccca ccctgctccg agagaacttc gtaggtcttg
      301 cgtcccatga cggccgtgtc gaattccttg aacagcgctg tgaaatcgat ggccggatcc
      361 atcacgatcc agtcgtattc gccattcggt cctgcgatga aaccgtccag gcttaccgcc
      421 acctggtagc gcagtcgtct gctcatgacc cagatccatt cactttacct tgattcccgg
      481 cgagcagata gaataatgtc aacatgcagg ctgggcgaat ctcctgggtt atcgttctgt
      541 gcggcgcgct gattcccatt gcgtcggcac aatccaactc cactccgccg ggagtgcgct
      601 ctttcttcgt gcaacactgt ttcgcctgcc atagcaacaa agcaaagacg gccgggctcg
      661 atcttgaatc ccccgtcacg gaccataccg tgtgggaaga tgtgctgcga aaaattcgaa
      721 gcggtgagat gcccccggct ccgatgcccc ggccccgccc agccgatatc gcggcagtat
      781 cgaagtggat tgcgtccgtt cctctccggc cgggaacaca gccacagaag gtggaccccg
      841 gccgtgtcac cgcacgccgt ttaaataggg ccgagtacga taatacgatc cgcgatctgc
      901 tggccgtcga ttaccgcccg gcgcgcgatt ttccggccga cgatagcggt tatggcttcg
      961 acaacatcgc cgacgtgctg tcgctctcac ccgttctgat ggagaagtat tttgccgctg
     1021 cggaggagat cagccggatc gttctgccag gcaacgtggc tccaaagccg acggccgagc
     1081 gatataaaca ggagcaaacc gccggcgcca aaccaggttt tctcgagatc cggcaccggt
     1141 tccccgccga ggccgaatac gacctgcggg ttgtgctcgg tggcgtccgc ccgccccgct
     1201 cgcctgaaat ccggatggct ttcctgctcg atggacggga gttgcagact ttcggcgtcg
     1261 atccgggacg aggcaaaccg cgcagtttcg ggacgcgcct ggtggtaaag gcgggcgaac
     1321 gcaacttgcg agcggtgttc ctgaacgatg attacaaccc ggaagaacat tccttgaatg
     1381 ccacggatcg ccaccttact attgactaca ttgaagtccg ggggccttac aatattcgcg
     1441 gtactctgac cgaatcgcag cagaaggtgt ttgtttgcgg gcatgtcgcg gaccaccatc
     1501 atccggactg cgtgcgagat aacctctccg atctggcacg ccgggcattc cggcgcccgg
     1561 tttccaaaga ggagaccggc cctctcgttc gcttcgtcaa actcgcagcg gaacagggag
     1621 acacgctcga agatgggatg cgccttgccc tgcaggcgat tctggtctcg ccccagtttc
     1681 tgtttcgcat cgagcgcgac ccgccgggcg gtgccgcgca ccctgtcacc gatttcgaac
     1741 ttgctt
//
LOCUS       DASTMK010000449         4867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_439004, whole genome shotgun sequence.
ACCESSION   DASTMK010000449 DASTMK010000000
VERSION     DASTMK010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439004"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFQ79_19130"
     CDS             <1..950
                     /locus_tag="VFQ79_19130"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEU0141845.1"
                     /translation="YYPQRYETKQVFRLMDLALTQLEKNRDSIELALNASDIERINKK
                     GKIAAVLDLEGGFDLDGDLGILRDLYRLGLRSTQLSAHNWTSNFADSCCAAPKFHGLN
                     DHGRAVIREMNRLGMVINISHASDEAVAQAIEWSSVPVVATHHGLRSLNNIPRNMPEE
                     LMRKLAAKGGVMGFQIGNEFHNRKAFDWRTKHAGKPFWDTDHIAKREQQPSIEEVDRR
                     VAPQFPMVGLNMPDDVIFAVDDWIAVVEKAIGIMGEDHVALGSDFDGGPTLPRGMRDI
                     SDTPMLTDAMLRRGWSEQRIRKFLGLNLMRVFRQITEKK"
     gene            971..3460
                     /locus_tag="VFQ79_19135"
     CDS             971..3460
                     /locus_tag="VFQ79_19135"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU0141846.1"
                     /translation="MKRLIPILCAVALATPAMPLDLVRNGKSAYSIVIAPDASASERH
                     GAEELQKFLEEISGARLPVTTEPRSNMVLVGRSPTLDTLKLNIDFDELGPEGFALKTA
                     SKHLVIAGGRLRGTMYGVYSFLEKLGCRWFTPEVSRIPKMRSVSIRELDETQKPAFEY
                     REPFFAEASDAEWAARNKANGMLMKLDAARGGKIQYFPFVHSFEALVSPKRYFQDHPE
                     YFSLIDGRRRAERSQLCLTNPDVLRIGIESVERWIAEHPDANILSVSQNDWTGWCECD
                     NCRRVEEEEGGAHSGPLLRYVNALASEIEKKHPDKLIDTLAYWYTEDPPLKARPRKNV
                     RIRLCPIGACEAHPYEQCKYNAYFMKNLRAWAKITNQLYIWHYNTNFAHYLLPFPDFD
                     ELAADIPMYHRTGVVGLFMEGSPSSAGGAENAELRSYVMARLMWDSGTDVEKDIDEFH
                     QAYYGEASRPMRAYFDLMHREVRVPPRGKGHHIWIYDRPSAPYLNEEFLAEATKLFRQ
                     AEAAASSDVVRNRIRKARLGIDYIDLTRAKKFSVEGEWYRPANLNALKDRWQTFVAAV
                     RSFGMTHISESTLLSKDEQDFETFVRPYAVASLENERLRVHVAPELGGRVTHIIDKRN
                     GRDLLLHPEPGSKQYPNLGGLTVAPYADYVSRQPWSTSWSLNRNASRQELSLTGVSEN
                     GLELRRTLKLDGPMLRTQTVLENKGSAPVEAVLQSRWEVDPGSLEHVIVSYGRQDGAT
                     VEKQLIEPEKQPAGTEFYTGAEQPNGEWQVLNHAGGPAIVNRFAKDQVARCFLNWTAK
                     SENRVGLAVASPRRTLQPGETLTLEADYGVK"
     gene            3661..4848
                     /locus_tag="VFQ79_19140"
     CDS             3661..4848
                     /locus_tag="VFQ79_19140"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0141847.1"
                     /translation="MTRHRFLALFAFFCAPHIAMVTTSAQTPGGPVVIGEMVQFESNI
                     LKESRSLLIAKPDGYEASTERYPVLYLLDSELYFNYTSAIEAFLARNDRVPKTLVVGV
                     VSGDTARRTHDLTPPSTAENDNRFSPGNGGADAFLSFLADELIPFVERTYRTRPYRLL
                     IGHSFGGLFAVYAMAKEPALFNGYIVADPSIYWNNQAVVAQAESFLTHTKSLQADFYM
                     AASGGSDRPPGEIARLRAAFAKSAPAGFRWSLDLMQQEDHMSIALPSLYHGLETIFAE
                     WRLANPLELYNKAGIEGIHRRYQETGRRFGYPERTTPPFMVSLLVAALMSAGRLEEAS
                     QVLLHDPKTYPPPWNQLDALARRYESRGNPKDAAQYYLLSLRENPQNDHAKKKRPIWE
                     FSG"
BASE COUNT         1079 a         1418 c         1411 g          959 t
ORIGIN      
        1 actactaccc gcagcggtac gagaccaagc aagtgttccg gctcatggat ctcgctctca
       61 cacaactcga gaagaatcgc gattccatcg agctcgcgct gaacgcatcc gacatcgaac
      121 ggatcaacaa gaaagggaag atagccgctg tcctcgacct ggagggagga ttcgacctgg
      181 acggcgatct ggggattctc cgcgatttat atcgcctggg gcttcgctcc acgcagcttt
      241 ccgcgcataa ctggacgagc aatttcgccg attcctgttg cgccgcaccg aagtttcatg
      301 gcttgaacga tcacggccgg gcggtgatac gggagatgaa ccggctggga atggtcatca
      361 acatttcgca tgcatcggat gaagccgtag ctcaggccat cgaatggagc agtgttccgg
      421 ttgtggcgac gcaccacggc ctacgctcgc tgaataacat tccacgcaac atgccggaag
      481 agctgatgcg gaagctggcg gctaagggcg gcgtaatggg atttcagatc ggcaacgaat
      541 ttcacaaccg caaggccttt gactggcgga cgaagcacgc gggcaagccg ttctgggata
      601 ccgaccacat cgctaaacgc gagcaacagc cttcgatcga ggaggttgac cgcagagtgg
      661 cgccgcagtt tccgatggtc ggcttgaaca tgccggatga cgtgatcttc gcggtggatg
      721 attggatcgc ggtggtggaa aaagcgatcg gcatcatggg cgaagatcat gtcgcgctgg
      781 gtagcgattt cgacggcgga cccacattgc cgcgcggcat gcgcgatatt tccgatactc
      841 cgatgctgac ggatgcaatg ctgcgccgcg gctggtccga acaacgcatt cggaaatttc
      901 tggggctgaa cctgatgcgc gtgtttcgtc agattacgga aaagaaataa ggcgaaaggc
      961 atggcgggat atgaaaagac ttatcccaat cctctgcgct gtggctctcg caacgcctgc
     1021 aatgccgctc gatctggttc ggaacggtaa atcggcttac agtatcgtca tcgctccgga
     1081 cgcttccgcc tccgaacgcc acggagcgga agagttgcag aagtttctcg aagagatctc
     1141 cggcgcacgt ctgccggtta ccaccgaacc ccgttcaaac atggtgctcg tgggccggag
     1201 ccccactctg gatacgctga agctgaacat cgacttcgac gaactcggcc cggagggctt
     1261 tgcgctcaaa accgccagta aacacctggt catcgcgggc ggccgcctgc gcggcacgat
     1321 gtacggtgtc tactcctttc tcgaaaaact tggctgccgt tggtttacgc cggaggtgag
     1381 ccggatcccc aaaatgcgca gcgtttcgat ccgcgaattg gacgaaacgc aaaagccagc
     1441 ttttgagtat cgcgagccgt tcttcgccga agcatccgac gcagagtggg ccgcgcggaa
     1501 caaagcgaac ggaatgctca tgaaactcga cgccgcgcgg ggcggcaaga tccagtactt
     1561 tccgttcgtg cactcgtttg aagcgctcgt atcgccaaaa cggtatttcc aggatcatcc
     1621 cgagtacttt tctctgatcg acggcaggcg ccgcgccgaa cgcagccaac tctgcctcac
     1681 gaatcccgac gtgctgcgga tcgggatcga gtctgtcgag cgctggatcg ccgagcaccc
     1741 cgacgcaaac atcctgtccg tctcgcaaaa cgactggacg ggttggtgcg agtgtgacaa
     1801 ttgccgccgc gtggaagaag aggaaggcgg cgcccactcc ggccctttgc tgcggtatgt
     1861 aaatgcgctc gcgtcggaaa tagaaaagaa acatcccgac aagctgattg acacgcttgc
     1921 ctattggtac accgaagatc cacctttgaa ggcgcgtcct cgcaaaaacg ttcgcatccg
     1981 cttatgtccc atcggtgcct gcgaggcgca cccctatgag cagtgcaagt acaacgccta
     2041 tttcatgaag aacctgcggg cgtgggcgaa gatcaccaac cagctttaca tctggcacta
     2101 caacaccaac tttgctcatt acctgcttcc ctttcccgat tttgatgagc ttgccgccga
     2161 catcccgatg taccatcgca cgggcgtcgt cggactgttc atggaagggt cgccgagcag
     2221 cgccggtggg gcggaaaacg cggagttgag atcctatgta atggcccgtc tcatgtggga
     2281 ttccggcacg gacgtcgaaa aggacatcga tgaattccac caggcctatt acggtgaagc
     2341 gtcacggccg atgcgcgctt atttcgattt gatgcaccgc gaggtgcgcg tgccgccgcg
     2401 gggcaaggga catcacatct ggatttatga ccgtccctcg gcgccctatc tgaatgagga
     2461 atttctcgct gaggccacga agcttttccg ccaggccgag gcggcggctt cgagcgatgt
     2521 cgtgcgcaac cggattcgca aagcgcgcct gggaatcgac tatatcgacc tgacccgcgc
     2581 gaagaagttt tccgtcgaag gcgaatggta tcgccccgcc aacctgaacg cgctgaagga
     2641 ccgctggcag acattcgtag cggccgtgcg aagcttcggc atgacgcaca tcagcgaatc
     2701 cactctgctg tccaaagacg agcaggactt cgaaaccttc gtacggcctt atgcggtcgc
     2761 ctcgctcgag aatgaacggc tgcgcgtgca tgtcgccccc gaactgggcg gccgcgtgac
     2821 tcacatcatc gataaacgca acggacgcga cctgttgctg catcctgaac cgggttcaaa
     2881 acagtatcca aacctgggcg ggctcaccgt cgcaccgtat gccgactacg tctcgcgcca
     2941 gccatggagc acgtcctgga gcctgaatcg aaacgcatcg agacaagagc tttcgctcac
     3001 gggtgtctct gagaacggac tcgaactgcg gcgcacgcta aaactggacg ggccgatgct
     3061 acgcacgcaa accgtgctcg aaaataaggg ctcggctccg gtcgaggcgg tgcttcaatc
     3121 gcgttgggag gtcgatcccg gttcgctcga acatgtgatt gtgtcgtacg gcagacagga
     3181 tggagcaacc gtcgaaaagc agttgatcga gccggagaag caacccgcgg gcaccgagtt
     3241 ttacaccggc gccgaacaac cgaacggcga atggcaggta ttgaatcacg ccggaggacc
     3301 cgccattgtg aaccgcttcg ccaaagacca ggtagcacgc tgctttctga actggaccgc
     3361 taaaagcgaa aatcgagtcg gcctcgccgt agcctcccca cgccgcacac tccaacccgg
     3421 ggagaccctc acactcgaag cggactacgg agtgaaataa tagggccgtc cccgtcgctc
     3481 cacatagctt gaaaccatgc ttgccgggct gctacgagtg ccgccggctc gcactgtgta
     3541 ggatccggga tttctggctt gccggccgaa agcccgcaag tgccattacc tttcagttaa
     3601 gagatagaat cgtctcaaaa tagcagcggg accagcgaag tgccgacgag gaggatgtcc
     3661 atgacgaggc atcgatttct tgctcttttc gcctttttct gcgcgccgca catcgcgatg
     3721 gtcactacct cggcccagac accaggtggg ccggtggtaa ttggcgagat ggtgcagttt
     3781 gagtcgaata tcctaaagga atcgagatcg ctgctcatcg cgaaacccga cggatatgaa
     3841 gccagtaccg aacgctaccc ggttctctac ctgctcgaca gcgaattgta ctttaattac
     3901 acgagcgcaa tagaggcttt cctcgccagg aacgaccgcg ttccgaagac gctcgtggtc
     3961 ggggttgtca gtggagacac agcgcgccgt acgcacgatt tgacgccacc ctcgacagcc
     4021 gagaacgaca accggttctc acccggcaac ggcggagcgg atgcgttcct ctcattcctt
     4081 gccgatgaat tgattccctt cgtggaaagg acctatcgta cgcggcccta tcgactgctg
     4141 atcggccact cgttcggcgg gctgttcgcc gtttacgcaa tggctaaaga gcctgcgctc
     4201 ttcaatgggt acattgtcgc cgacccgagc atttactgga acaaccaggc ggtagtcgcg
     4261 caggcagagt cgtttctcac acacaccaag tctcttcagg cggatttcta catggcggca
     4321 tccggcggct cggaccggcc tcccggtgag attgcgaggc tgcgcgctgc gtttgccaaa
     4381 tcggctcccg caggattccg ctggagcttg gatttgatgc agcaggagga tcacatgtcg
     4441 atcgctctgc cgagcctcta tcatggattg gagactatat tcgccgagtg gcgtctggcg
     4501 aatccgttgg aactgtacaa taaggccggg atcgaaggga ttcacaggcg ctatcaggaa
     4561 accggccgga gattcggata ccccgaacgg accacgcccc ccttcatggt ttccctattg
     4621 gttgcggctc tgatgagcgc agggcgcttg gaagaggcgt cgcaggtgct cctccacgat
     4681 cctaaaacat atccccctcc ctggaatcaa ctggatgcct tggctcgccg ttacgaaagt
     4741 cgtggtaacc ccaaagatgc ggctcagtac tatcttctgt cactcagaga gaatccgcag
     4801 aatgaccatg caaagaaaaa gcggcccatc tgggaattca gtgggtagcg tgataatggg
     4861 cgggtgc
//
LOCUS       DASTMK010000450         9938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_55821, whole genome shotgun sequence.
ACCESSION   DASTMK010000450 DASTMK010000000
VERSION     DASTMK010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55821"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..392)
                     /locus_tag="VFQ79_19145"
     CDS             complement(138..392)
                     /locus_tag="VFQ79_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEU0141848.1"
                     /translation="MKREYDFSKAKRGPVIPVPKGKTRITIRLDEDVVAWFRSQVEES
                     GGGNYQSLINDALRHHIARAREPLEETLRRVVREEIRRAS"
     gene            complement(376..675)
                     /locus_tag="VFQ79_19150"
     CDS             complement(376..675)
                     /locus_tag="VFQ79_19150"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEU0141849.1"
                     /translation="MYNCAYVLIEWDPGKARLNLNKHGVSFADAVIALEDDGALTVRD
                     PFSAEERWLTMGLDGLGRVLVVVYTWRGETLRLISARKSTPRERRCYEDANETGI"
     gene            832..1809
                     /locus_tag="VFQ79_19155"
     CDS             832..1809
                     /locus_tag="VFQ79_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141850.1"
                     /translation="MATVSPRIRYRIASSHYAMKQIYRKVPEIRKSASLEERRRKELF
                     AEYSSLLRKAVDQFDIGSPGSKPGFNLLWGDIEAQHHGKMQIFDKSDKSSPTHDLLEP
                     LGGILSGTNQAILVNGSLNDASAATANAAYLHGKRNSVFGNATTAHVLNITEPGDYPW
                     AYNSAFMENLMERMAVALGTAVAAKEQEGGKVSVYTHSFSGLAGALLGASVPTELRDK
                     VRIFLLNPAQLVEPYELQSALQQTDVETHVIQGSRDPITSLIRSKAEAAHMTGDEMET
                     GFYSGPPGLIVGNPNVWLKLVPTGHSFTAIAESAIQRESEGETNTKESQ"
     gene            complement(1825..2562)
                     /locus_tag="VFQ79_19160"
     CDS             complement(1825..2562)
                     /locus_tag="VFQ79_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0141851.1"
                     /translation="MAQAVAESVERGEQLRRDGIVIRTYDLWKTYVMGDQEIHAVSGV
                     DVQIRRGEYVAIMGPSGSGKSTLMNLIGCLDTPTRGEYYINGSLVSSMNDDELARIRN
                     REIGFVFQTFNLLPRATALHNVELPLIYSGMPADVRLEKAKMALRHVDLEQRMYHKPN
                     ELSGGQRQRVAIARALVNDPSILLADEPTGNLDSATGNEIMALFASLHQQGNTIVLVT
                     HEHDIAVHAHRVVHVRDGKVEKDEKIK"
     gene            complement(2576..3994)
                     /locus_tag="VFQ79_19165"
     CDS             complement(2576..3994)
                     /locus_tag="VFQ79_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0141852.1"
                     /translation="MKRKWKVLLACTLAIVVAGGIFAAVKWSRRGIVPVQTGKVDRRD
                     LASIVTASGEIKPRNYINIGSNASGPARIIDLLVKEGDPVKKGQLLAQLESVQPEAEV
                     AAQRAALSTSEAESAAAEASLRAADENIRTAAASVERARAELERARLNWSRAEQLFKD
                     QLIAKQEFDQQKAEYDALNAGLGEAEARLSQAKAQRAQTASQLAASQKRIVQAQANLR
                     RANDFLQRTQSRAPLDGVVTNLPVRVGETVVPGIQNSPASLLMTIADMSLITAEVKVD
                     ETDIVNVLLNQTADITIDAIPSRTFHGHVMEIGNTAILRSTGLAASQSAISSQEAKDF
                     KVVIALDTPPEEIRPGLSCTAKITTATRSRVLAIPIQALTIRKKGDLEPPAGKSVQAA
                     GKVDEAAQKAKNEEIQGVFAISGEKAEFRKVDTGITGATDIEVVSGLNEGDRIVTGSY
                     QVIRTLRNGARVKIDSGTPTKN"
     gene            complement(4115..4384)
                     /locus_tag="VFQ79_19170"
     CDS             complement(4115..4384)
                     /locus_tag="VFQ79_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141853.1"
                     /translation="MNRKTVLLLIAAGIALVGYLLYSSLALRQHRVEVCIEFGGRRTC
                     RIALAQTEAQAIRQATQNACALMAGGVTDTIACENTPPSSIRRLE"
     gene            complement(4385..5326)
                     /locus_tag="VFQ79_19175"
     CDS             complement(4385..5326)
                     /locus_tag="VFQ79_19175"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU0141854.1"
                     /translation="MNDETRAQIEALEREIAGLRDRESGTEVRIGQLEEQLRTLQEQP
                     VEKHPSWQRVLLARHPKRPHSLDYIQRLFTDFQEIHGDRAFSDDPAIVAGLGFFEGRP
                     VAVVGQQKGRDTKQKLFRNFGMPKPEGYRKALRVMQLAAKFQRPVITMLDTPGAYPGI
                     DAEERGQAEAIAVNLREMSRLGVPIIVVVIGEGGSGGALALGVGNLVLMLENAVYSVI
                     SPESCAAIIWRDAGKAELAAAALRLTATDLEQLGLIDTIVPEPPGGAHEDHDAAAELL
                     RAGIRKSLETLSSPTPQELIESRYRKFRNMGSFFMEG"
     gene            complement(5402..8866)
                     /gene="dnaE"
                     /locus_tag="VFQ79_19180"
     CDS             complement(5402..8866)
                     /gene="dnaE"
                     /locus_tag="VFQ79_19180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU0141855.1"
                     /translation="MSETPFVHLHCHTDYSLLDGACEIEQLMEVVEQQKMPAIAMTDH
                     GNLFGAVEFYNKAVAKGIHPVIGCEVYVAQQGYKNRSDTNRYNHLVLLAETQEGYRNL
                     IDLVSTGYLEGFYYKPRIDKDLLSSHAKGLIAMSACLKGDINETLLSERYDEARRLAH
                     EYQDMFGRGNFFLEIQDHGLDPDRKVVPLVNRLSQESGIPMVATNDSHYLRRQDSRAH
                     EILLCIQTGKTFSDASRMRWDNPEFYLKSRDEMLRMFGEMESALDATWDIAQRCRVKL
                     NKVSEPFPRFEVPPEHSTDTYFEYVARQGFEKRRARLEPLSRAGRLKHDLGEYSERLD
                     REIKMIQQMKFSGYFLIVWDFIRFAKSQGIPVGPGRGSAAGSLVSYAMEITDIDPLEY
                     GLLFERFLNPERISMPDIDIDFCTHRRGEVIQYVTEKYGREQVAQIITFGTLGAKAAI
                     KDVGRVLDMTFAEVDRLTKLVPNQLNIRLAEAIAKEPGFEEAARKDARISDVLEVAQR
                     LEGKCRNASVHAAGVVISPVPLKELVPLYRTNREEIVTQFDMKGLEKLGLLKMDFLGL
                     TTLTIIRDAVELIRKHRGVQLEIEEIPLDDPKTYEIFSKGFTSGVFQFESAGMRDILR
                     RYQPARIEDLCALNALYRPGPIQGGMIDDFIDRKHGRREVAYDLPELKRVLEETYGVI
                     VYQEQVMQISNLLAGYSLGDADILRRAMGKKKLEEMSAQRDRFLAGAAERGFPPKKIA
                     RIFDLMEQFAGYGFNKSHSAAYAYLAYVTAHLKAHYPIEFMAALLTSETGNTDKIVKY
                     INECREMGIRVLPPDVNSSEWNFTPDGEAIRFGLGPIKNLGQNAVEAIMAARREVGRF
                     QSLYEFFEKVDPAAINRRMIESLIKAGAMDGLGGTRSQLTAGLDGAMEHGQRVWKDRA
                     SGQAGLFAAMFAEQGPVEKELPQVPDWQVADQLAGEKEMLGFYVTGHPLDEYMHKVSE
                     LATHDSESVEGLPRGTEVAIAGILTSIQRKRNKEGKLWASMQLEDLKGHLEAPIFASS
                     YEANLQFLVEDKAVLVRGSVLPEENGPPKISVREIIPLELARVSYPSLISIRVRIGSE
                     KSGEVPAALQQLFRRKRGDTDVRLRLEKPRDFSMILDVSTKVRPDKEFQAEVARLCGP
                     EALEILGS"
     gene            complement(8949..9866)
                     /locus_tag="VFQ79_19185"
     CDS             complement(8949..9866)
                     /locus_tag="VFQ79_19185"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0141856.1"
                     /translation="MKVFEQPLRGIVVPLVTPLKSANELDEGGLERLIEHVLAGGVSA
                     LFILGTTGEGTSLSGSLRRLVIERTAKQLAGRVPLLTGITDTAFSETLDLACFAANHG
                     AHGVVYAGPAYSPVSQSELIANVARMAESSPLPLFLYNMPSHTHTFFELETVVRLSEL
                     PCVAGLKDSSGNLIYFERTSIEMEKRRPDFSLLMGPEEILAAALLGGAHGGVNGGANL
                     FPELYVQLYEACRAGDWAGAARLNRTVLQISAGIYASNSCASSYLRGLKCALSLAGIC
                     SEAMAEPYGPLEAAERERIAEFLAVAELK"
BASE COUNT         1846 a         3086 c         2862 g         2144 t
ORIGIN      
        1 gcttgcgttc tgcgccgtgg taagcaggcg ccgatcgaat ttgtggtcac attcggtggg
       61 gaccccaaac gtacgagatt actcctcaca agtccgcttg tggcatcggt cgaacgcagg
      121 caagtcagcc gcctaagtca actagcccgc cggatttcct ctctgacaac gcgcctcaag
      181 gtttcttcaa gaggttcgcg ggcgcgcgct atatgatgcc ttagggcatc gttgatcagg
      241 ctttgatagt tgccgccgcc tgattcttcg acttggcttc gaaaccacgc gaccacgtct
      301 tcgtcgagcc ggatcgtgat tcttgtcttt cccttgggga ccggtattac gggcccgcgc
      361 ttcgccttcg aaaagtcata ttcccgtttc attcgcatcc tcgtagcacc ggcgctcacg
      421 cggcgtcgac ttccgcgccg agatcagacg taacgtttcg cctcgccagg tgtagacaac
      481 aaccagcacg cgcccaagac catctaatcc cattgtcaac cagcgctctt ctgccgaaaa
      541 cggatcccgt accgttaatg ccccatcgtc ctcaagagca ataaccgcgt ccgcaaacga
      601 aacgccgtgt ttattgaggt ttagccgcgc cttgccagga tcccattcta tcagcacata
      661 tgcacaattg tacaccaata ttgaggtctg gccagcccct cctcctgccc gatcaacgct
      721 tggaggcgcc tgaatccccc cgggtatata ctttcaggca ccactggaaa ctcagaagga
      781 ggatgccaca tggctagtcc cgctagaacg ttccgatctg ccagaccgct aatggcgact
      841 gtaagcccgc gcatacggta ccggatagcg agttctcact acgcgatgaa gcaaatctac
      901 cggaaagtgc cggagatcag gaagagcgct tccctggaag agcgccgccg gaaggaactc
      961 tttgccgagt actcgtccct gctgcggaaa gccgtcgatc aatttgatat cggctcaccc
     1021 ggcagcaagc ccggattcaa tctcttgtgg ggcgatatcg aagcccagca tcacggcaaa
     1081 atgcagatct tcgacaagag cgacaaatcc tctcccactc acgacctgtt ggagccgctg
     1141 ggcggcatcc tctccggcac caaccaggcc attctggtaa acgggtcgct caatgatgcc
     1201 tcggccgcca cagcgaatgc ggcttatctt cacggcaagc ggaattccgt ttttgggaac
     1261 gctacaacgg ctcacgtatt gaacatcacc gaacccggcg attacccctg ggcttacaac
     1321 agcgccttca tggaaaatct gatggagcgc atggccgtcg ccttggggac cgcggtcgcc
     1381 gccaaggaac aggaaggcgg caaggtttcc gtgtatacgc atagcttttc tggactggcg
     1441 ggtgctctct taggagcatc ggtgcctacg gagcttcgcg acaaggtccg gatcttcctg
     1501 ctgaatcccg ctcaactggt ggaaccgtat gaattgcaga gcgccctgca acagacggac
     1561 gtggaaacac acgtcatcca ggggtcgagg gacccgatta cctcattgat ccggtcgaaa
     1621 gccgaggccg cccacatgac aggcgacgag atggaaacgg gcttctattc cggtccgccc
     1681 ggcctgatcg tcggcaatcc caacgtctgg ctgaaactgg ttcccaccgg gcacagcttt
     1741 acggcgattg cggaatccgc gatccagagg gaatccgaag gggaaacaaa caccaaagag
     1801 agccagtgac ggcgcgctgg gcggttattt gattttctcg tccttctcga ctttgccgtc
     1861 gcggacatgc acgacgcggt gagcgtgcac ggcgatgtcg tgctcgtgag tgaccaggac
     1921 aatcgtgttg ccctgctggt gaaggcttgc aaacagggcc atgatctcgt tgcctgtcgc
     1981 cgagtccagg tttcccgtag gctcgtcggc gagcaggatg gaggggtcgt tcaccagggc
     2041 gcgggcaatg gcaacgcgtt ggcgctggcc gccggatagc tcgttgggct tgtggtacat
     2101 gcgctgttcg agatccacgt gacgcagcgc catcttggct ttctccaggc gtacatccgc
     2161 cggcattccg gaataaatca acggcagttc gacgttatga agagccgtag cgcgcggcag
     2221 caggttgaag gtctggaaga cgaacccgat ttcgcggttc cgaatccggg ccaactcgtc
     2281 gtcgttcata ctgctgacca ggctgccgtt aatgtagtat tcgcccctgg tgggcgtgtc
     2341 caggcagccg atcaagttca tgagggtcga cttgccggaa ccggacggtc ccataatggc
     2401 gacgtattct ccacgcctga tctgtacatc cactccggaa acggcgtgaa tttcctgatc
     2461 gcccatgacg tatgtcttcc aaaggtcata cgtccggatc acaatgccgt ctcgtcttaa
     2521 ctgttcgccg cgctcaacgg attctgctac tgcctgtgcc atgctgtctt atccttcaat
     2581 ttttggtcgg ggttccgcta tcaattttca cacgggctcc gttgcgaagg gttcgaatca
     2641 cctggtaact gcccgtaacg atccggtcgc cctcgttcaa gccgctgact acttcaatat
     2701 cggtagcccc tgtaatgcca gtatccactt tccggaattc cgctttttcg ccggagatcg
     2761 caaacacgcc ctggatctct tcatttttag ctttttgtgc cgcctcgtct actttgccgg
     2821 ccgcttgcac actcttgccc gcgggcggct ccaggtcgcc ctttttccga atggttaaag
     2881 cctggatcgg gatggctaat acacggctac gcgtggcggt ggtgatcttg gccgtgcagg
     2941 agaggccggg ccggatctct tccggcggcg tgtcgagtgc gatcaccact ttgaaatctt
     3001 tcgcctcctg gctggagatg gcgctctgtg acgccgccag gccagtggat cgcaggattg
     3061 ccgtgttgcc gatctccatg acgtggccgt ggaaggtccg gctggggatc gcatcgatcg
     3121 tgatatcggc cgtttgattg agtagcacgt tgacaatgtc cgtctcgtct actttgacct
     3181 cggccgtgat cagagacata tccgcgatcg tcatgagcag gctggcggga gagttctgga
     3241 tgccgggcac caccgtctcg cccacccgga cgggcaggtt cgtcactaca ccatccaaag
     3301 gggcccggga ttgggtccgc tgcaggaaat cgttggcgcg gcggaggttc gcctgagctt
     3361 gtacaatccg cttctgcgac gcggcgagtt gcgaagcggt ctgtgcccgc tgtgctttgg
     3421 cctgcgagag acgcgcttcg gcttccccca aaccggcgtt gagcgcatcg tactccgctt
     3481 tttgctgatc gaattcctgc ttcgcaatca actggtcttt gaaaagttgt tcggcacggc
     3541 tccagttcag ccgggctcgc tccagttccg cgcgggcgcg ttccacggac gccgcggcgg
     3601 tgcgaatatt ttcgtcggct gcgcgaaggg aagcctctgc ggcggcggat tccgcttcgg
     3661 acgtgctgag agcagcccgc tgcgcggcta cctccgcctc cggttgtact gattccaact
     3721 gggccagaag ttgccctttt ttcacggggt ctccctcttt aaccagcaga tcgatgatgc
     3781 gggccggacc gctcgcgttc gaaccgatgt tgatataatt ccgcggttta atctcaccgg
     3841 aggcggtcac gatagaagcg agatcccggc ggtctacctt gcccgtttgc accggaacaa
     3901 tgccgcggcg gctccatttg acggcggcga aaattccgcc cgccacgaca atcgccaatg
     3961 tacaggcgag cagaactttc cacttacgct tcactgcgca aaactcccgt tagtacagac
     4021 gtacaccgtc tctcgttcgt tcgatgtttc tatgctatca cgcgaaaaac accgcttcgg
     4081 gtgcgaaatt gccgttcccg cctgccggag cttcttattc gagccggcga atactcgaag
     4141 gcggggtgtt ttcgcacgca atggtatccg taacaccgcc cgccatcagg gcacaggcgt
     4201 tctgggtggc ttgccggatg gcctgggctt cggtctgagc cagggcgatg cggcaggtgc
     4261 gccggccacc gaattcgata caaacttcca cccggtgctg gcgcagggcg agggaggaat
     4321 aaagaagata gcccaccagg gcgatgccgg cggcgataag gagcagaacc gtctttcgat
     4381 tcacctaccc ctccatgaag aagctgccca tgttccggaa tttccggtac cggctttcga
     4441 tcaactcctg cggcgtcggg gacgagagcg tctcgagaga tttccggatg ccggcccgca
     4501 gcaattcggc cgcggcgtcg tgatcttcgt gagcgcctcc gggcggctcg ggaacaatgg
     4561 tgtcgataag cccaagctgt tcgaggtcgg tcgcggtgag ccgcagcgcg gcggcggcga
     4621 gttccgcttt tcccgcatcg cgccagataa tggcggcgca gctttccgga gagatcacgc
     4681 tgtacacggc gttttccagc atcagcacca ggttgcccac ccccagcgcg agcgcgccgc
     4741 cgctgccgcc ctcgccgatc accaccacga taatgggcac acccaggcgc gacatctcgc
     4801 gcaggttcac cgcgatcgcc tcggcctgtc cgcgctcttc ggcatcgatg cccgggtagg
     4861 cgcccggggt atccagcatc gtaatcaccg gccgctggaa cttggctgcc agttgcatta
     4921 cgcgcagcgc cttccggtat ccctcgggct tcggcatgcc gaagttgcgg aacagcttct
     4981 gtttggtgtc gcggcccttt tgctggccca caaccgccac cggcctgcct tcgaaaaagc
     5041 ccaatccggc gacaatggcg ggatcgtctg aaaacgcgcg gtcgccgtga atttcctgaa
     5101 aatccgtgaa cagccgctgg atgtagtcga gcgagtgcgg gcgcttggga tgccgcgcca
     5161 gcagtacgcg ctgccacgaa ggatgcttct ccaccggctg ctcctgtagc gtgcggagct
     5221 gctcttccaa ctggccaatg cgaacttccg ttccggattc ccggtcgcgc aagccggcaa
     5281 tctcgcgttc gagcgcttca atctgtgccc tggtttcgtc gttcaatggt gtgtgaatcc
     5341 tccgccccgc tcctgcggct tcggcaacgc ctgattctac caggatggcg gcagttcggg
     5401 tttagctgcc taagatttcg agcgcctccg gcccgcagag cctcgccact tcggcctgga
     5461 attccttatc ggggcgaacc ttagtgctca catccagtat catcgaaaaa tcacggggct
     5521 tttcaagccg cagccggacg tccgtatcgc ctcgcttgcg tcgaaagagc tgctgcagcg
     5581 ccgccggcac ttccccggac ttttccgagc cgatgcgcac gcggatggaa atcaggctgg
     5641 ggtacgatac gcgcgcaagt tcgagcggga tgatctcccg cacggagatc ttgggcgggc
     5701 cgttctcctc cggcagcacg gaacctcgca ccagaacggc tttgtcttcg accaggaact
     5761 ggagatttgc ctcgtagctg gaggcgaaga tgggtgcttc caggtggcct ttcaaatctt
     5821 caagctgcat cgatgcccac agtttgcctt ctttattgcg ctttcgctgg atggaggtca
     5881 atatgccggc gatagccact tcggttccgc gcggcaatcc ctccacgctt tcgctgtcgt
     5941 gcgtggccag ttcgctcacc ttgtgcatgt actcatctaa aggatgcccg gtgacataga
     6001 acccgagcat ctctttctcg ccggcgagct gatcggccac ttgccagtcc ggcacttgcg
     6061 ggagctcttt ttccacgggc ccctgttcgg cgaacatggc ggcgaacagg ccggcctgcc
     6121 cgctggcgcg atctttccag acgcgctgtc cgtgctccat ggcgccgtcg agaccggcgg
     6181 tcaattgcga ccttgtccct cccagaccgt ccatcgcacc ggccttgatc aggctctcga
     6241 tcatcctccg gttgatggcc gccggatcga ctttctcgaa aaactcgtac agcgattgga
     6301 aacgtcccac ctcgcgccgc gccgccatga tcgcttccac cgcgttctgt cccaggttct
     6361 tgatggggcc cagtccgaac cggatcgcct cgccatcggg ggtaaaattc cattcgctcg
     6421 agttcacgtc cggcggcaat acacgaatgc ccatttcgcg gcactcgttg atgtatttca
     6481 cgatcttatc cgtgttgccg gtttccgacg tcagcagggc cgccatgaac tcgatcggat
     6541 agtgcgcctt caaatgcgcc gtgacgtagg ccagataggc gtacgccgcc gagtgcgatt
     6601 tgttgaagcc gtagccggcg aactgctcca tcaggtcgaa aatcctggcg attttcttcg
     6661 gtgggaagcc ccgttccgcg gcgccggcca ggaagcggtc gcgctgcgcc gacatctcct
     6721 ccagtttctt cttgcccatg gcgcggcgca ggatgtcggc atctcccagt gaatagccgg
     6781 cgagcagatt cgagatctgc atcacctgct cctggtacac gatgacgccg taggtttctt
     6841 cgagtacgcg tttgagttcc ggaagatcgt aagctacttc cctgcggccg tgtttgcggt
     6901 cgatgaagtc gtcgatcatc ccgccctgga tggggccggg ccggtagagc gcgttcagcg
     6961 cacacaaatc ctcgatacgc gccggctggt accgccgcag aatgtcgcgc atgccggccg
     7021 actcaaactg aaatacgccg ctggtgaagc ctttggagaa gatctcgtag gtcttggggt
     7081 catcgagcgg gatttcctca atctccagtt gcacgcccct gtgtttgcgg atcagctcga
     7141 cagcgtcgcg aataatcgtg agcgtcgtca accccaggaa gtccatcttg aggagaccca
     7201 gtttctcgag ccccttcatg tcgaactggg tgacgatttc ttcgcggttt gtgcggtaga
     7261 gcggcaccag ttccttcagc ggaaccggcg aaattacgac cccggcggca tgcaccgatg
     7321 cattccggca cttgccttcc agccgctgcg ccacctcgag cacgtcgctt atccgcgcgt
     7381 cttttcgcgc cgcttcctcg aatccgggct ccttggcgat cgcctccgcg agccggatgt
     7441 tgagctggtt gggcaccagt ttcgtcagcc ggtccacctc cgcaaacgtc atatcgagca
     7501 cgcggcctac gtctttgatc gccgcttttg cgccgagggt tccgaacgtg atgatctggg
     7561 ccacctgctc gcggccgtat ttctcggtga cgtactggat gacctccccc cggcggtgcg
     7621 tgcagaaatc gatgtcgatg tccggcatgc tgatccgctc ggggttcagg aagcgctcga
     7681 aaagcagccc gtattcgagg ggatcgatgt cggtgatctc catcgcatag ctgaccaggc
     7741 tgcccgccgc cgatccgcgt cccggaccca cggggatacc ctgtgacttg gcgaaccgga
     7801 tgaagtccca gacgatcaga aaatagccgg agaatttcat ctgctggatc atcttgattt
     7861 cgcgatccag ccgttcggaa tattcgccga ggtcgtgttt cagcctcccg gcgcggctca
     7921 acggctccag gcgcgcgcgc cgcttttcga agccctgccg ggccacatat tcgaaatacg
     7981 tatcggtgct atgttcgggc gggacttcga agcgggggaa cggctcgctc accttgttga
     8041 gcttcacgcg gcaacgctgc gcgatgtccc aggtcgcatc gagcgcggac tccatctcgc
     8101 cgaacatccg caacatctcg tcgcggctct tcaggtagaa ctccgggttg tcccagcgca
     8161 tgcgggaagc atcgctgaac gtcttcccgg tctggatgca gagcaggatc tcgtgcgccc
     8221 gcgagtcctg ccggcgaagg taatgcgaat cgttcgtcgc gaccatcggg atgccgctct
     8281 cctgcgagag acggttcacc agcggaacga ctttccggtc gggatcgagg ccgtggtcct
     8341 gtatctcgag aaagaaattc ccgcgcccga acatgtcctg gtactcgtgt gccaggcggc
     8401 gggcctcgtc ataccgctcg gagagcaggg tctcgttgat gtcgcccttc aggcaggcgg
     8461 acatcgcaat caaccccttc gcgtgggacg aaagcaggtc cttgtcgatc cgcggcttgt
     8521 agtagaagcc ctccaggtag ccggtggaaa ccaggtcgat caggttgcgg tagccttcct
     8581 gcgtttccgc cagcagcacc aggtggttgt agcggttcgt gtccgagcgg ttcttataac
     8641 cctgctgtgc gacatacacc tcgcatccga tgacgggatg gatgcctttg gcgacagctt
     8701 tgttgtagaa ctccaccgcg ccgaacaggt tgccatggtc ggtcatggcg atggcgggca
     8761 tcttttgctg ctcgaccacc tccatgagtt gctcgatttc gcaagcgcca tcgagaaggg
     8821 aataatcggt gtgacagtgc aaatgcacaa agggtgtttc ggacatcgcg gatcacttct
     8881 atagtagccc gtctgcgcgg cggcgccggg caaaaccgtg tggctacgga tcccttccgc
     8941 ttttgccgct acttcaattc cgccaccgcc agaaactccg caatacgttc gcgctcggct
     9001 gcctcgagtg gaccgtacgg ctccgccatc gcttccgaac agatccccgc cagcgaaagc
     9061 gcgcatttca accctctcag gtagctcgac gcacaggagt tcgaggcgta tattccggcg
     9121 ctgatctgca aaacggtccg gttcagccgg gccgccccgg cccagtctcc cgcgcggcac
     9181 gcttcgtata gctgcacata cagctcggga aaaagattcg cgccgccatt gacaccgccg
     9241 tgagcgcctc ccaacaaggc cgccgccagg atctcttccg gtcccatcag gagcgagaaa
     9301 tcgggccgcc gcttttccat ttcaatagag gtccgttcaa agtagatcag gtttccggaa
     9361 ctgtccttca ggcccgccac acatggaagc tcagacagcc ggacgaccgt ctccagttcg
     9421 aagaacgtgt gcgtgtggct cggcatgttg tagaggaaga gcggaagcgg agaactctcc
     9481 gccatgcgcg caacattcgc gatcaactcg gactggctca caggcgagta ggccgggccg
     9541 gcatacacta ctccgtgcgc cccgtgattc gccgcgaagc aggcgaggtc caacgtttcc
     9601 gaaaaagccg tgtcggtaat gccggtcagc agaggcactc gcccggcaag ttgtttcgca
     9661 gtcctctcga tcacgaggcg gcggaggctg ccgctaagcg atgtcccttc acccgttgta
     9721 cccagaatga agagagcgct cacgccgccg gccagaacgt gttcaatcag acgttccagg
     9781 ccgccttcat ccaactcatt tgccgatttc agcggcgtga ctaacgggac cacgatcccg
     9841 cgcaacggtt gttcgaaaac tttcattttg tttggtccca cggaatcgtt tccaaactta
     9901 catcctctgt ctgatatttc attatagtga atgtcgtt
//
LOCUS       DASTMK010000451         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_24587, whole genome shotgun sequence.
ACCESSION   DASTMK010000451 DASTMK010000000
VERSION     DASTMK010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24587"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /locus_tag="VFQ79_19190"
     CDS             <1..2042
                     /locus_tag="VFQ79_19190"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0141857.1"
                     /translation="PGRNVLAAVVWNFGEHAPQAQVTNETGFLLQGDTERERIADTNA
                     EWKCVRNEAYEPIPFTSAQMRGYYVAGPGDRVTASAYPWGWERPGFDDSGWKPARLGT
                     RAAPRYSRDSPSRWMLVPRSIPMMEESPEKPLTVRRTNAGTDWKYPAKVPAQTKIRIL
                     FDQAYLTTAYPEVTVSGGRNARLRMSYAEALWIPGKREKGNRNEVAGKEFIGNYDEFI
                     ADGGQHRLFRPLWWRTYRYLELEIETAGDPIVVEKLGGTYTGYPFERKARFESDSGEI
                     GTLLDVGWRTARLCAHESYMDCPYYEQLQYAGDTRIQALVSLYMSGDARLMRNAIEQL
                     DSSRTPEGATFSRAPSRLQQYIPPFSLWWIGMVHDYWRYQDDPAFVKEMLPGVRAILS
                     FFEARQKQNGSLGVLPWWNFVDWTKEWRNGVPPAAADGSSAPLDLQLLLAYGWAAGME
                     DALGMPPLGAEYRRRAEKLRTSAGSLYWDSRRRLYADTPGRTEFSQHTNALAVIAGLV
                     KPEEAAGFVKRVAADSSLVQASIYFRHYLHSAFNQAGAGDDYLDQLNQWRTMLQRGLT
                     TWAEQADPTRSDCHAWGSSPNYEVFRTVLGIDSAAPGFRRVIIRPFPGKLSRASGVIP
                     HPGGEIAVRYERKQDGLEAEVRLPAGVEGEFVWRGESRPLKPGANTVALRVPLVQ"
     gene            2244..>2976
                     /locus_tag="VFQ79_19195"
     CDS             2244..>2976
                     /locus_tag="VFQ79_19195"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0141858.1"
                     /translation="MRISDLRLHPVAVADAPLRSSYGRHAPYALRTIVELVTDEGLIG
                     ISETYGGDRPLAALEAARSRVVGMDPFQLTRLWLDFSGDAAAEETDVAGGRSQTYLVP
                     GENPLDRHTRTYAAIEVACLDVIGKALGKPVCDVVGGRARDAVDFSAYLFYKHAGGGG
                     SGADAREDEYGECLDPKSMVRQAKQMIRQYGFREIKLKGGVLDPDIEIATIRALREEF
                     GPSFPLRIDPNCAWSVETSLRVGESL"
BASE COUNT          577 a          875 c          989 g          535 t
ORIGIN      
        1 ggcccggccg gaatgtgctc gcggccgtag tctggaattt cggcgagcat gcgccgcagg
       61 cgcaggtgac gaatgagacc ggcttcctgc tacagggcga cacggaacgc gagcgcatcg
      121 cggacacgaa cgcggaatgg aagtgcgtgc gcaacgaagc ctatgagccc attccgttca
      181 ccagcgctca aatgcggggt tattacgtag ccggtccggg cgaccgcgtg acagcgtccg
      241 cttatccctg gggctgggag cgccccggct tcgacgactc cggctggaag ccggcgcggt
      301 taggcacgcg cgccgcgccg cgttattcgc gagacagccc gagccggtgg atgctggttc
      361 cgcgcagcat tcccatgatg gaagagagtc cggaaaagcc tctgacggta cgccggacca
      421 atgccggtac cgattggaag tatccggcca aggtgcccgc gcagacgaaa atcaggatcc
      481 tgttcgatca agcctatctc acgacagcgt atccggaggt gaccgtcagc ggcggcagga
      541 atgcacggct gagaatgagc tatgccgaag ctctgtggat tccgggcaag cgggaaaaag
      601 gcaaccggaa cgaggtcgcg ggcaaggaat tcatcggcaa ctacgatgaa ttcatcgcgg
      661 atggcggcca gcaccgcctg ttccggccgc tgtggtggcg cacgtaccgt tatctcgaac
      721 tcgaaatcga gacggcgggc gaccccatcg tcgtggagaa actgggtggc acttacacgg
      781 ggtacccgtt tgagcgcaag gcccggttcg aatcggattc cggcgagatc ggaaccctgc
      841 tggatgtggg ctggcgcaca gcgcgcctgt gtgcgcacga gtcttacatg gattgcccct
      901 attacgagca gcttcagtat gccggcgaca cgcgcattca ggcgctggta tcgctctaca
      961 tgagcggcga cgcccgcctg atgcgcaatg ccatcgagca actggactcg tcgcgtacgc
     1021 ccgagggcgc caccttcagc cgcgctccct cgcggcttca gcagtacatt ccgcccttct
     1081 cgctttggtg gatcggcatg gtccacgact actggaggta tcaggacgat ccggcctttg
     1141 taaaagagat gctgccggga gtgcgagcga tactgtcgtt cttcgaagcc cgtcaaaagc
     1201 agaacggctc gctgggtgtt ctgccctggt ggaattttgt ggattggacg aaggaatggc
     1261 ggaacggcgt gccgccggcc gcggcggatg gctcgtcggc gccgttggat cttcaactcc
     1321 tgctggccta tggttgggcc gccgggatgg aagacgctct cgggatgccg ccgctcggag
     1381 ccgaataccg gcggagggca gagaagcttc gtacgtccgc aggcagcctc tattgggaca
     1441 gccggcggcg gctgtatgcc gacactccgg gcaggaccga attttcacaa cacaccaacg
     1501 ccctggcggt gattgcgggg ctggtgaaac cggaggaagc tgccgggttc gtgaaacgcg
     1561 tcgcggcaga ttcgtcgctg gtgcaggcgt ccatttattt ccgccactat ttgcattcgg
     1621 cgtttaacca ggctggcgcc ggcgacgact atctcgatca gttgaaccag tggcgcacga
     1681 tgttgcagcg cggcctgacg acttgggcgg agcaggccga cccgacgcgg tcggattgcc
     1741 acgcatgggg atcgagtccg aactacgaag tgtttcgcac cgtgctcggg attgattcag
     1801 cggcgccggg ctttcgccgt gtgatcatac gcccgttccc cggtaagctg tcgcgagcct
     1861 ccggcgtcat ccctcatcct ggcggtgaga ttgccgtccg ctatgaacgg aagcaggacg
     1921 ggttggaagc cgaggtccgg cttccggcgg gagtcgaggg cgagtttgtg tggcgtggcg
     1981 agagccgtcc cttgaagccg ggtgcgaaca cggtagcgct tcgggttcca ttggtacaat
     2041 gaggattcca ttcaaaacct gaaggagaca gttttggcag agaacacaga aaacaccggc
     2101 agcaacgaga agctttataa agcggagtac caggggagcg gtaccttcct catcagcaag
     2161 ccgaagcgta aggtcaaaaa gcagcgccag acgaaagtaa gaaacccgca gcgaggagcc
     2221 cgaacatagc ccacccgcgc cccatgcgca tctccgacct tcgtttgcat cctgtcgccg
     2281 tggcggatgc accgcttcgc agctcgtacg gacgccatgc tccgtacgcg cttcgcacga
     2341 tcgtcgagct ggttacggac gaagggctca tcggaatcag cgagacttac ggcggcgacc
     2401 gtccgctggc ggccctggaa gccgcgcgga gccgtgttgt cggcatggat ccgttccagc
     2461 tcacgcggct ctggctcgat ttttccgggg atgccgccgc tgaagaaacg gatgtagcgg
     2521 gcggccggtc gcagacctat ctcgtaccag gcgagaaccc gctggaccgg catacgcgaa
     2581 cctacgccgc catcgaggtc gcgtgcctgg atgtcattgg gaaggccctg ggcaagccgg
     2641 tttgcgatgt cgtaggcggg cgggcgagag acgcggtgga tttctcggcc taccttttct
     2701 acaaacacgc gggcggcggc ggatccggcg ccgatgccag ggaagacgag tacggcgaat
     2761 gtctggaccc gaagtcgatg gtgcgccagg cgaagcagat gatcaggcag tacggcttcc
     2821 gggaaatcaa gctgaaaggc ggcgtgctcg atcccgatat cgagatcgcc accatacggg
     2881 cgttgcgcga ggagttcgga ccttccttcc cgctgcgtat cgatcccaat tgcgcctggt
     2941 ctgtcgaaac atccctccgc gtgggcgaaa gcttgc
//
LOCUS       DASTMK010000452         1856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_247336, whole genome shotgun sequence.
ACCESSION   DASTMK010000452 DASTMK010000000
VERSION     DASTMK010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247336"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFQ79_19200"
     CDS             <1..1320
                     /locus_tag="VFQ79_19200"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141859.1"
                     /translation="AGLPHEDAWTSYLKDIDTAGEEPLKSPSLVQIAGTQPESFEQWY
                     RTNVYEQRQNGYATITITLPLGDITATQLRKVADLARTYVKDTVRTTIEQNIVLRWVS
                     GADVPAVYAELDSVGLAAPGAGTIVDIASCPGTDTCKLGIASSRGLSGELRTRIIARG
                     MELDEAIRGLHIKISGCFNSCGQHHVADLGFYGVSRNKNGYTVPHFQVVLGGQWGENA
                     GAYGLAIGAVPSKRIPETVDRITGRYIAERLEGESFQAFIKRIGKAECKKMIDDLSQV
                     PPHDVDPSFYTDWADAREFTTGDLGVGECAGEVVSPFEFQSTACEREAFEAQLSLEAG
                     DIDKASRLAYGAMLNGAAALLRSRMITAGSPDEIVALFRTHFYDTQLFFDPFTGGKFA
                     HYFFHAHERAGEKRSAESVHRLIEETQLFIEACHSCYGKSLQQMVAV"
     gene            1320..>1856
                     /locus_tag="VFQ79_19205"
     CDS             1320..>1856
                     /locus_tag="VFQ79_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141860.1"
                     /translation="MPAAVMEIEHVNIKIFAKEPVEADLAGAIPMFHRWIQESVTPEL
                     LIDVADYRHVPSGPGVLLVGHEANYSLDLGGNRLGLLYNRKTAFPGDAAEKFKQAYNA
                     AVQVCALIEADPAFQGKLKFDLKHFELIVNDRLLAPNTDETWEALKPEIEKFCQVQFG
                     GAFVLRREGGPRERFRVVI"
BASE COUNT          385 a          532 c          583 g          356 t
ORIGIN      
        1 gccggtttgc cgcatgagga cgcctggacg tcttacctga aagacatcga tacggccggt
       61 gaagagccgt tgaaaagtcc aagccttgtt cagatcgcgg ggacccagcc ggaaagtttc
      121 gaacagtggt atcgcacgaa cgtctatgag cagcggcaga acggatatgc caccatcacc
      181 atcactctgc ctctggggga catcaccgca acacagttgc gaaaggtggc ggacctggcg
      241 cgcacgtatg tgaaggacac ggtccggact acgattgaac aaaacatcgt attgcgatgg
      301 gtcagcggag cagacgtgcc ggcggtctat gcggagctcg actccgtcgg gctggcggcg
      361 cctggagcgg gcaccatcgt ggatatcgcg tcctgccccg gcaccgacac ctgcaagctg
      421 ggcattgctt cctcccgcgg cctttcggga gagcttcgga cgcggatcat cgcgcgcggc
      481 atggaattgg acgaagcgat tcgcggtttg catatcaaga tcagcggatg cttcaactcg
      541 tgcggccagc accatgtcgc cgacctcggt ttctacggcg tcagccggaa caagaacggc
      601 tatacggtgc cacatttcca ggtggtgttg ggcggccagt ggggggaaaa cgccggcgcc
      661 tacgggctgg cgatcggcgc ggttccgtca aagagaatcc cggagaccgt tgaccgtatt
      721 acgggccgct acatagcgga acgcctcgaa ggagaatctt tccaggcttt catcaagcgc
      781 atcgggaaag ccgagtgcaa gaaaatgatc gacgacctgt cgcaggttcc gcctcacgat
      841 gtggaccctt cgttctacac ggattgggcc gatgcgaggg agttcaccac aggcgacctg
      901 ggagtcggcg agtgtgccgg cgaggtcgtt tcgcccttcg agttccaaag taccgcctgc
      961 gagcgggaag ctttcgaagc gcagctcagc ctggaagcgg gcgacatcga caaagcttcc
     1021 cggctggcgt acggcgccat gttgaacgga gccgccgcgc tgctgcgctc ccgcatgatc
     1081 acggccggtt ctccggatga aatcgtcgcc ttgttccgca ctcacttcta cgacacgcag
     1141 ttgttcttcg acccgtttac cggtggcaag ttcgcgcatt acttcttcca cgcgcatgag
     1201 cgcgcaggcg aaaagcgcag cgccgaatcg gtgcaccggc tcatcgaaga gacgcagctt
     1261 ttcatcgagg cctgccatag ttgttacggc aagagcttgc agcagatggt ggcggtctga
     1321 tgccggcggc ggtaatggag atagaacacg tcaatatcaa aatctttgcg aaggagccgg
     1381 tcgaggccga tcttgccggc gccatcccga tgttccaccg ctggatacag gagagcgtca
     1441 ctcccgaact gctcatcgac gtagcggatt accgccacgt ccccagtggt cccggggttt
     1501 tgctggtggg ccatgaagcg aattacagtc tggatctcgg cgggaatcgc ctgggcctgc
     1561 tatacaaccg caagacggcg ttcccgggcg atgccgccga aaaattcaag caggcctata
     1621 acgcggcggt gcaagtctgt gccctcatcg aagccgatcc cgcattccag gggaaattga
     1681 agtttgatct gaaacatttc gagctgattg tgaacgaccg cctgctggcg ccgaataccg
     1741 atgaaacttg ggaagcgctg aagccggaga tcgaaaagtt ctgccaggtg caattcggcg
     1801 gcgctttcgt gttgcggcgc gaaggcgggc cgcgtgagcg attccgcgtt gtgatc
//
LOCUS       DASTMK010000453         7341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_55979, whole genome shotgun sequence.
ACCESSION   DASTMK010000453 DASTMK010000000
VERSION     DASTMK010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55979"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFQ79_19210"
     CDS             complement(<1..700)
                     /locus_tag="VFQ79_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141861.1"
                     /translation="MNRRDFLAANTAALASPVLSQTAAQPHQPIPRAKWIENGIIDAG
                     GSHEPHSFVIRRGGYRMDAHERYQEAQSEEVLRRLKSQGVEVFHTHLYKGAGMAHEKA
                     EMEDARRVAAIAHRLGMRVDSYLQWNTMMYETFFAEEPRAKNWVQRDEAGVPIKLVYG
                     FQQSFRYRPCFANAEYLEYLGRIVRYAVEEVKTDFIHFDNFDLNPEPDSCHCESCVNG
                     FRRFLRTKYTAAERK"
     gene            complement(711..2879)
                     /locus_tag="VFQ79_19215"
     CDS             complement(711..2879)
                     /locus_tag="VFQ79_19215"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0141862.1"
                     /translation="MQKLSRREFAKRAAFTGAVFGGVPVHLFGERGPSYAEQHPDMLV
                     SYLSGKLNTLAGKWDRERDALRTAAQLESRNRFVREKFREMVHGWPDKTPLAPIVVRT
                     NERNGYRVENVMFQSRPNFWVTGNLYVPSGKGPFPGIISPCGHYPLARMEPEYQFAYM
                     NLVLNGFVVLAFDPVGQGERRQYWNPQTGETEVASASTYEHSMPGQVLLLMGEDLTHY
                     RIWDGMRAIDYLLTRPEVDKEKIGCAGHSGGATLTIFISSLDERVRCVAVNQGGTGHR
                     WPIKASPGSRLGPSDVEQNIFPAAVHGIDVCDLLTAIAPRPLLLTIENYSPAFRRTAD
                     HVKARYAQFDTAERFGTEEANDPHSWTVKLRQSTTDWFSRWFSGRRGPDREPDLAPEN
                     PETLYCTPNGSVRYSQQGETIFSLILKNGVTVTPSRKVPASAAELGEFRNRLAGEIRE
                     LIRYRAPEGPLGARHIVTTPRKGYKVENVEFLTEPGIHIPAWVFVPEKRNAEPPTVFV
                     HEAGKEVEGQEFGLLEGLAREGHQVIAVDVRGIGETTPPHSPGDRSGPFSHLFSVETA
                     MAYLSWYMDEDLFGMRVRDVVRSVEYALSRPDTGAASVRLVGKGAGALWALYAAALDP
                     RVETVVAHGGLVSYASLTRVDRYRQTAGVFVRDVLKHFDLPYVAAAIADRRLILSSTV
                     DPMNERLDPAAVRQAYDWTAAAYRNAGAVDRFQITESYEL"
     gene            complement(3102..4082)
                     /locus_tag="VFQ79_19220"
     CDS             complement(3102..4082)
                     /locus_tag="VFQ79_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0141863.1"
                     /translation="MSVVTIPGILCLFAAFPQVQQPPPEETVPTIQVDVDFVNILFSV
                     RDKKGALIPNLERDDFTVFEEGKQQEVKYFVRETDLPLTMGLLVDVSRSQENLIDIER
                     RAAYEFFSQVLKEKDLAFLISFGSESELLQDFTGSPRLLQRGLENLRVKSSVGGIHPG
                     PVPTANRPKGTVLYDAVYLAATEKLRSEVGRKALILITDGNDQGSMMSRDQAVEAAQR
                     SDVIIYSIFYEDRSFGSSDGFYYSGDEGTLRRMSEETGGRVFRVDRKHTLDSIFDEIQ
                     NEMRSQYAIGYTPLNEARDGSFRRIEIRTKNKDLKVQARKGYYAGARPKK"
     gene            4128..4736
                     /locus_tag="VFQ79_19225"
     CDS             4128..4736
                     /locus_tag="VFQ79_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU0141864.1"
                     /translation="MRKAILHLKTVDPVLGGIIEKVGKYRIEYLEPEFSTLVRSIVYQ
                     QLSGKAAATIYNRLAAAVGDGGRLSPDLILALGANEMRRVGLSQQKTAYILDLAERTL
                     SGELDFATLRRLTDQQVIGKLTEVKGIGVWTAHMFLIFALRRANVLPSGDLGIRVAIQ
                     KAYSLPELPPVKQVAEIATSWHPYCTVASWYLWRSLEEKAGL"
     gene            complement(4737..6317)
                     /locus_tag="VFQ79_19230"
     CDS             complement(4737..6317)
                     /locus_tag="VFQ79_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0141865.1"
                     /translation="MSQRFDVAIIGGGHNGLVAAAYLAGAGRRVVVLERRELVGGCSV
                     TEEVWPGFRVSTAAYLTSLLQERIIQDLDLPRFGYAVDAKDPAFFSPFPDGRYLFTWQ
                     DQRRTLAEIARFSQRDAERYPEYEAQLERISATVESLLLTTPPEFPPQRAGDYLDYLK
                     LIGKTRGLQRGDLIALVKIFTQSAAEYLDEWFESEQVKVTLATDGVIGANGGPRSPGT
                     AYILLHHCMGGVAGKRGLWGFVRGGMGAVSEAIAGAAKAAGAIIRTNAEVEKIRIVGN
                     RATGVVLAGGEEISAAIVASNLDPHLTFLRLVGEQHLDGEFASRIRQYRSEGTSLKMN
                     LALSGLPEFRVLPGAPGPQHRATMHICPSVEYVEKAWDDAKYGRPSQAPLVEMTIPTM
                     YDASLAPAGKHIMGIFLQYAPYTLREGDWDSLREPFAERVLDTIEEYVPNIRSIIIDR
                     QVLTPLDLERQFGLTGGNIFHGEMSLDQMFFLRPVAGCARYKTPVPGLYLCGSGAHPG
                     GGVMGAPGYNAARAMLKG"
     gene            6426..>7341
                     /locus_tag="VFQ79_19235"
     CDS             6426..>7341
                     /locus_tag="VFQ79_19235"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0141866.1"
                     /translation="MISLTRFLLVLLLALPLLAQNPVVSGRVTDSSGAVVPNTQVQLK
                     NAGTGVLNTTTTNDEGYFVLPPVPPGFYDVVAAATGFKEARVDAVRLEIGQTRTINLQ
                     LQPGDVVESITVADVAPLITVNRPDRGTVVENKFLTSIPLNIRNPYLLLATVPGVTTG
                     RLAGDNTASQSTTNNFRINGGRGSTSEILIDGAANTGTYNNQVSAMPQLDAVQEFKVN
                     TSPYAAEFGRTGGGVVSFSIKSGSNDLHGTFHEFLRNSVLDAAGFNSNRAGLTEKPSF
                     KRNQFGLTSGGPVWLPKLYDGRNRTFWFF"
BASE COUNT         1444 a         2232 c         2163 g         1502 t
ORIGIN      
        1 ccttgcgttc ggcagccgta tacttcgtcc gaagaaaacg gcggaatccg ttcacacagc
       61 tctcgcaatg acaggagtcg ggctcgggat taagatcgaa attgtcgaaa tggatgaagt
      121 ccgtcttgac ttcctctact gcgtagcgca cgatccggcc gaggtattcc aggtattccg
      181 cattggcaaa gcacggccgg tagcggaagg actgctgaaa cccatacacg agcttgatgg
      241 gaactcccgc ctcgtcgcgc tgcacccagt ttttcgcgcg cggttcctcc gcaaagaacg
      301 tctcgtacat catggtgttc cattgcagat aagagtcgac gcgcattccg aggcggtgtg
      361 cgatggccgc cacgcgtctt gcgtcttcca tctcggcctt ctcgtgagcc atgcccgcac
      421 ccttgtaaag atgcgtgtga aacacttcaa ctccctggct cttcaggcgg cgaaggacct
      481 cttcgctctg cgcttcctgg tagcgctcgt gtgcatccat gcggtaacca ccacggcgga
      541 tcacgaacga atgaggctca tgcgatccgc ctgcatcgat gatgccgttc tcgatccatt
      601 tcgcgcgtgg gatgggttgg tgtggctgtg cggccgtttg cgatagaacg ggactggcca
      661 gagcggcggt attagcagcg aggaaatcgc gtcggttcat aggagtcagc tcacagttcg
      721 taggattctg ttatctggaa tcggtccaca gcgccggcgt tgcggtaagc cgctgccgtc
      781 cagtcgtaag cctggcgcac cgcagccgga tcgagacgtt cgttcattgg gtctaccgtt
      841 gaagacagga taagccgccg gtctgcgatg gcggccgcca cgtacggcaa atcgaaatgc
      901 ttcagtacat cgcgtacgaa aacgccggcg gtctggcggt accggtcgac gcgcgttaag
      961 ctcgcatagg agacaaggcc gccgtgggcc acaacggtct cgacgcgcgg gtcgagagcg
     1021 gctgcgtaga gggcccaaag cgcccccgcg cctttgccta ccagccggac gctggccgcg
     1081 cccgtatccg gccggctcaa cgcgtattcc acgctgcgaa ccacatcgcg tacgcgcatg
     1141 ccgaagaggt cttcgtccat gtaccaggac aggtaggcca tggcggtttc tacgctgaac
     1201 aggtgcgaaa atggtccgct gcggtcgccc ggcgaatggg gcggtgtcgt ttccccgatc
     1261 cctcgcacgt ccacggcgat cacctgatgc ccttcgcgcg caagcccctc cagtaatccg
     1321 aactcctggc cctccacttc ttttccggct tcgtgcacga atacggtagg aggctcggcg
     1381 ttgcgctttt cgggcacaaa gacccaggcc gggatgtgga tgccgggttc ggtcaggaac
     1441 tcgacatttt cgaccttgta gcctttgcgc ggcgtggtga caatgtgtcg cgcaccgagc
     1501 ggtccttcgg gggcacggta acgaatcagc tcgcgaatct ccccggccag ccggtttctg
     1561 aattcgccta gctccgccgc gctggcgggc actttgcggg atggggtgac cgtcactcca
     1621 ttcttcaaaa tcagggaaaa gatggtctcg ccctgctgcg agtagcggac agagccattc
     1681 ggcgtgcagt acaaggtctc aggattttcg ggcgccagat ccggctcgcg gtccgggcca
     1741 cggcggccgc tgaaccatcg cgagaaccag tctgttgtgg actggcgcag cttcacggtc
     1801 caggagtgcg ggtcgttggc ctcttccgtc ccgaatcttt cggcggtgtc gaattgcgcg
     1861 taacgagcct ttacatgatc ggccgtgcgg cggaatgcgg gcgagtagtt ctcaatggtg
     1921 agcagcagcg gcctgggcgc gatcgccgtt aaaaggtcgc agacatcgat gccgtgaacc
     1981 gccgcgggaa aaatattctg ctcgacatcc gacggcccaa gcctgctccc cggcgatgcc
     2041 ttgatgggcc agcgatgccc tgtgccgccc tggttgacgg caacgcagcg aacgcgctcg
     2101 tccagcgaac tgatgaagat agtcagcgtc gcgccgccag agtgtccggc acagccgatt
     2161 ttttccttgt ccacttccgg gcgggtcaac aggtaatcga tagcgcgcat gccgtcccag
     2221 atgcggtagt gcgtcaggtc ttcccccatc agcagcagca cctggccggg catggagtgc
     2281 tcgtaagtcg acgcggaggc aacttccgtt tcgcccgttt gcggattcca atattgccgc
     2341 cgctcgccct gtccgacagg atcgaatgcc agaaccacga acccattgag cacgaggttc
     2401 atgtaggcaa actggtattc cggttccatt cgcgccagcg gatagtggcc gcatggcgaa
     2461 ataattcctg gaaaggggcc ttttcccgag ggtacgtaca gattgccggt tacccagaaa
     2521 ttcgggcggc tttggaacat cacattttct acgcgatagc cgtttcgctc gttggtccgc
     2581 accacgatgg gtgcaagcgg cgttttatcc ggccagccgt gcaccatctc gcgaaacttt
     2641 tcgcggacga accggttgcg ggattcaagc tgcgcggcgg ttcgtagagc atccctctcc
     2701 cggtcccatt ttccggccag agtgttcagt ttccctgaca aataagagac cagcatatcc
     2761 gggtgctgtt cggcataaga tggcccgcgc tccccgaata aatggaccgg cactcctcca
     2821 aataccgcac ccgtgaaggc cgctctcttc gcgaactccc gcctgctcag tttttgcata
     2881 actgtggtcc ctacttctat cacgtaagaa cccaattatc cgatttcctg gcctgcgccg
     2941 ccacttgccg gcggagattg gggtgtgcga caggaaagcg atagcggagc cgcgacggtc
     3001 acggagcggt ggtcgccaac cgctcgctaa cgctcacggc tcagttacac accccgtgga
     3061 gatcagggga tgttatcgaa accggtcagg agggattttg atcacttctt cggccgcgct
     3121 ccggcgtagt agccctttcg ggcctggacc ttcaggtcct tgttcttcgt ccggatctct
     3181 atacgtcgaa acgagccgtc ccgggcttcg ttcagcggcg tatagccgat cgcatactgg
     3241 ctgcgcattt cgttctgaat ctcatcaaag atggagtcca gcgtgtgctt gcgatctacc
     3301 cgaaacacgc gcccgccggt ctcttcggac attctgcgaa gggtgccctc gtcaccgctg
     3361 taataaaatc cgtcgctgct gccaaacgag cggtcttcat agaagatgct gtagatgatg
     3421 acgtcggacc tctgcgcagc ctcaacggcc tggtcccgcg acatcatact gccctggtca
     3481 ttgccgtccg tgatgagaat gagtgccttg cggccgactt cgctccttag tttctcggtt
     3541 gccgcgaggt agacggcatc gtacaacacc gtgcctttcg gccggttcgc agtcggcacc
     3601 ggaccgggat ggatgcctcc cacggaagac ttcacccgca gattttcgag acctcgttgc
     3661 agcagacgcg gagaaccagt gaagtcctga agcaactcgc tttccgatcc gaagctgatg
     3721 aggaacgcga gatccttctc ttttaagacc tgggagaaaa actcgtaagc ggccctccgt
     3781 tcgatgtcga tcaggttttc ctggctgcgg ctgacgtcca ccagcaggcc catggtgagc
     3841 ggcaggtcgg tctcacggac aaaatacttc acctcctgct gcttaccctc ttcgaacacc
     3901 gtgaaatcgt ctctttccag attcgggatg agcgcaccct tcttgtcgcg aacagaaaaa
     3961 agaatattga cgaaatcgac gtccacttga atggtgggca cggtctcttc aggcggcggc
     4021 tgctgcacct gtgggaaggc ggcaaacagg cacaagatac ctggtatcgt cacgacgctc
     4081 atccatctca tacaatttag agatgcgccg tccggccttt gtgttccatg cgtaaagcta
     4141 ttcttcatct gaagacggtg gatcccgtcc tgggcgggat tattgagaag gtggggaagt
     4201 accgcatcga gtacctggag cctgagttca gcactctggt ccgaagcatc gtctatcagc
     4261 agctcagcgg aaaagcagcc gcaacaattt acaaccgcct ggctgcggcg gtgggagacg
     4321 gagggcgtct gtcaccagac ctgatcctcg ctctcggggc gaacgaaatg cgacgtgtgg
     4381 gtctttcgca gcagaaaaca gcttatattc tggatcttgc ggagcgcacc ctgtcgggag
     4441 agctcgattt cgccacactc cggcggctga cggaccagca ggttatcggc aagcttaccg
     4501 aggtaaaggg catcggcgtc tggaccgccc acatgttcct gatcttcgcc ctgcggcggg
     4561 ccaacgttct tccgtcggga gaccttggca tccgggtggc gattcaaaag gcgtactcgc
     4621 tgccggaact gccgccggtc aaacaagtcg ccgaaatcgc cacgtcgtgg cacccctact
     4681 gcaccgtggc gagctggtac ttgtggcgca gcctggaaga aaaagccggc ttgtaatcaa
     4741 cctttgagca tcgcccgcgc ggcgttatat cctggcgcgc ccatcacccc gcctcccgga
     4801 tgtgcgccgg acccgcagag atataggccg gggaccggcg ttttgtaacg ggcgcaacca
     4861 gccaccggcc gtaagaagaa catctggtcc aggctcatct cgccatggaa gatgttcccg
     4921 ccggtaagtc cgaactgacg ttccagatcc agcggcgtca gcacctggcg gtcgatgata
     4981 atggaccgga tattcggcac atactcctcg atggtgtcga gcacgcgttc ggcgaaaggt
     5041 tcacgcaggc tgtcccaatc cccctcccgt agcgtgtaag gcgcgtattg caggaagatt
     5101 cccatgatgt gcttcccggc gggagccagg ctggcatcgt acatggtagg gatcgtcatt
     5161 tccacgagag gcgcttgaga cgggcgcccg tacttggcgt cgtcccaggc cttctcgacg
     5221 tattcgaccg aagggcagat gtgcatggtg gcccggtgtt ggggacccgg cgcccccggc
     5281 agaacgcgaa attcaggcag gcccgaaagc gccaggttca tcttcagcga tgtaccttcg
     5341 gaacggtact gcctgatacg cgacgcgaat tctccatcga ggtgctgctc gccgaccagc
     5401 cgcaggaagg tcaggtgcgg atcgaggttg ctcgccacga tggccgcgct gatctcttcc
     5461 cctccggcaa gcacgacacc ggtcgcgcgg ttcccaacaa tgcgaatttt ttcgacctcc
     5521 gcattggtgc ggatgatggc tccggcagcc ttggcggcac cggcgatggc ttcggaaacc
     5581 gcgcccattc caccacgaac gaagccccat aacccgcgct tgcctgcgac gccgcccatg
     5641 cagtggtgca gcagaatgta ggccgtaccc ggggaacgcg ggccgccgtt cgcgccgata
     5701 acgccatcgg tggccagtgt cacttttacc tgctccgatt caaaccactc gtcgaggtac
     5761 tccgcagcgc tctgcgtaaa gatcttcacg agcgcgatca ggtctccgcg ctgtaacccc
     5821 cgcgtttttc cgatgagttt caaatagtcg aggtaatcgc cggcgcgctg cggcggaaat
     5881 tcgggaggcg tcgtcagcag aagcgattcc acggtcgcgg atattcgctc gagttgagcc
     5941 tcatattccg gataacgctc cgcgtcgcgc tgcgagaatc gtgcgatttc cgcgagcgtc
     6001 cggcgctggt cctgccaggt gaacagatac cggccgtcgg ggaatggtga gaagaaggcc
     6061 gggtcctttg catccacggc gtaaccgaag cggggcaggt cgaggtcctg aataatgcgc
     6121 tcctgcagca ggctggtgag ataagcggcg gtggagacgc gaaaccccgg ccaaacttct
     6181 tcggttacgg aacatccgcc caccagctcc cggcgttcga ggacaaccac tctgcgcccc
     6241 gctccggcaa gataagccgc ggcaactaac ccgttgtgcc cgcccccaat gatggctaca
     6301 tcaaatcgct ggctcacgaa gacctcatcc gtcaaatgta gatgacggcg ggacgcttat
     6361 caaggacagt gtctccccga aaccctgttg ttagcgtcct tgcggtttgc tatgatttcg
     6421 ccaacatgat ctcgcttacc cgttttctgc ttgttttgct acttgcgctt ccattgctgg
     6481 cgcaaaaccc ggtcgtatcc ggacgcgtga cggattcgag cggcgccgtg gtgccgaaca
     6541 cccaagtgca gttgaaaaac gccggtacgg gagttctcaa cactacgacg acaaacgacg
     6601 aggggtactt cgttctaccg cccgtgcccc cgggcttcta tgatgtggtt gctgccgcca
     6661 ccggctttaa agaagcgcga gtggatgctg tccgcctgga aatcggacaa acgcgcacca
     6721 ttaacctcca gctacaaccg ggcgatgtgg tggaatcgat cacggtggcg gacgtagctc
     6781 cgctgatcac ggtgaaccgc ccggaccgcg gaacggtggt ggagaacaag ttcctcacct
     6841 ccattcccct gaacatacgc aacccttacc tgttgctggc aaccgtgccc ggtgtaacaa
     6901 caggacggct ggccggtgac aacaccgcca gccaatccac tacgaataat ttccgcatca
     6961 atggagggcg cggatccacc agtgagatcc tcatcgatgg agcggcgaac accggtacgt
     7021 acaacaacca ggtctcggcc atgccgcagc tcgacgccgt acaggagttc aaagtcaaca
     7081 cctcgcccta tgccgcggaa ttcggccgca ccggaggcgg cgtggtcagc ttctccatta
     7141 agtccggcag caacgatttg cacggaacgt tccacgaatt tctgcgcaac agtgtgctcg
     7201 acgcggcagg gttcaattcg aaccgggcgg ggctcacgga aaagccgagc ttcaagcgga
     7261 atcagttcgg ccttaccagc ggtggtccgg tgtggctgcc gaagctgtac gatggacgga
     7321 accggacgtt ttggtttttc g
//
LOCUS       DASTMK010000454         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_183064, whole genome shotgun sequence.
ACCESSION   DASTMK010000454 DASTMK010000000
VERSION     DASTMK010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183064"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFQ79_19240"
     CDS             complement(<1..182)
                     /locus_tag="VFQ79_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141867.1"
                     /translation="MKWKDRNLRELAHIVCGNVKHFQYRSSSYITEFFADCDLDYIHD
                     GSTRWAWVASRLEKVLA"
     gene            complement(248..337)
                     /locus_tag="VFQ79_19245"
     tRNA            complement(248..337)
                     /locus_tag="VFQ79_19245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Ser,seq:tga)
     gene            442..1140
                     /locus_tag="VFQ79_19250"
     CDS             442..1140
                     /locus_tag="VFQ79_19250"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0141868.1"
                     /translation="MASTLMAIAAHPGDALFTMGGAVAQHVQQSGKGVFVSLTLGEKG
                     APKDIPTHKYGEMQREASERAAKMLGAGFELLTYPDAEIPLKDETALAVADVIRKHKA
                     DLVITHWSGSWHKDHQNCHLIVRDAIFYAALKTMERKDPPHSVRRLYYADNWEDAENF
                     KPDTYLDISGVFDKWMAACDAYPMWRGQTGFFRYNDYYGSLAVMRGCLSGFRHAVALM
                     TDPNERTNRVRSLG"
     gene            complement(1147..2655)
                     /locus_tag="VFQ79_19255"
     CDS             complement(1147..2655)
                     /locus_tag="VFQ79_19255"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0141869.1"
                     /translation="MTTHAQTPRPRIAIGGIVHESNSFFSVKTTLADFDRRTGDDPDA
                     ALEQWGKNNDEVSGFIEGGARFGLDLYPTLVAHATPSGPVTDEALDALTGDLLRRIQS
                     GGKFDGLLLALHGAMVAESYPHADGEVVRRLREALGSGFPIVVTHDFHANISPQVVEL
                     STALLNYKTNPHIDQKDRGIRAAWIMSGIVSRKWQPVQALVKPGMIYNIRFQNTNAEP
                     LAPIVEETRRLEEQDGILAASVAGGYQYADVPAMGASVVVVTNNDRELAERTAKRLSG
                     ELWDTRDRLALHLPDPAAAVSQAISSDLFPVVLVEMGDNIGGGASGDATFLLSELVRQ
                     KASGWFVALTDPRAAEAAAEAGIGGAFDMEVGAKTDRLHGDPVRIRGRVKSLHDGRFM
                     ETAVRHGGQRYYNQGLTAVIEEEGSTPDNQNLVMLTARRQVPFSLHQLIAHGVYPERQ
                     RILVVKAAVAFRAAYEPVAGRIIEVDTPGVTAVNPRRFTWKHVDPNLFGLEP"
     gene            2840..>3124
                     /gene="lepB"
                     /locus_tag="VFQ79_19260"
     CDS             2840..>3124
                     /gene="lepB"
                     /locus_tag="VFQ79_19260"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0141870.1"
                     /translation="MQDGSNHSEKTCAAAKPSTVQSLFSWARDLVLSVVIAAVVILFL
                     YQPVKVEGTSMMPALEDQERIFINKFVYSFGWGEIVRGDMVVFWYPADPSK"
BASE COUNT          622 a          980 c          896 g          626 t
ORIGIN      
        1 gccagcactt tctccagcct gctcgcaacc cacgcccagc gagtcgagcc atcgtggatg
       61 taatcaagat cacaatctgc gaaaaactca gtgatatagc tgcttgaccg atattgaaag
      121 tgcttgacgt taccgcacac gatgtgggca agttcccgga ggtttctgtc cttccatttc
      181 atttgcgtgg ctcaggtaag cgaacggttg ttgctgtccc acacgccgtt caggtagccg
      241 atgtgtgtgg cggaggaaga gggattcgaa cccccgagcg agttaccccg ctaacggttt
      301 tcaagaccgc cggtttcaac cactcaccca ttcctccact ccttagtgta ttccatgccg
      361 gctggttttc catcgatcga aggccggaca gcgcgtatga tggacctttc agtcccgcgg
      421 cggcgggacg gaggtttcat catggcatct actctgatgg ctatcgccgc acacccgggc
      481 gatgcccttt tcacaatggg cggtgccgtc gcccagcatg tccagcaaag tggaaaggga
      541 gtgttcgtca gcctgacgct cggcgaaaaa ggtgctccga aagacatccc cactcacaag
      601 tacggcgaaa tgcaacgcga ggccagcgag agggcggcta aaatgctcgg cgccggcttc
      661 gaactgttga cctatcccga cgcagagatc ccgctgaaag atgaaacagc tcttgccgta
      721 gccgacgtca tccgcaagca caaagccgat ctcgtcatca cgcactggag cggcagttgg
      781 cataaggacc accagaattg ccacctcatc gttcgcgacg ccatcttcta cgcggcgctg
      841 aagaccatgg agcgcaaaga cccgccgcac tccgtccgca ggctctatta cgcggacaac
      901 tgggaagatg ccgagaattt caaacccgat acctacctgg acatctccgg cgtgttcgac
      961 aagtggatgg ccgcctgcga cgcgtatccc atgtggcgcg ggcaaacagg cttcttccgt
     1021 tacaacgatt attatggaag cctcgcggtg atgcgcggct gtttatcggg cttccggcac
     1081 gccgttgcgc tcatgacgga cccgaatgag cgcaccaacc gcgttcgttc gctcggctga
     1141 cgagggctat ggctccaggc caaacaaatt cggatcgacg tgcttccacg taaaccgccg
     1201 cgggttcacg gctgtgacgc caggggtatc cacctcgatg atgcggccgg ccaccggctc
     1261 ataggccgcg cggaatgcca cagcggcttt caccaccaga atccgctggc gttccggata
     1321 aacgccgtgg gcgattaact ggtgaaggct gaacggcacc tgccgcctgg cggtcagcat
     1381 caccaggttc tggttgtcgg gcgtcgaacc ctcctcctcg ataaccgccg tcaggccctg
     1441 gttgtaatag cgctgcccgc catgccggac agccgtctcc ataaagcgcc catcgtggag
     1501 agatttcacc cgcccgcgaa ttcgcaccgg atcgccatgc agccggtctg tcttcgcgcc
     1561 cacttccatg tcgaaagctc caccgatgcc ggcttcggcc gccgcttccg ccgcgcgcgg
     1621 atcggtcagc gctacaaacc agccggacgc tttctggcga accaactcgc tcaacagaaa
     1681 ggttgcgtcg cctgaagcgc ctccgccgat gttatcgccc atctccacga gcaccaccgg
     1741 aaacaggtca ctcgaaatcg cctggctcac ggccgcggcg gggtctggaa ggtgcaacgc
     1801 caggcggtcg cgcgtatccc atagctcgcc ggacagccgc tttgccgtgc gctccgccag
     1861 ttcccggtcg ttgttggtca ccacgacgac acttgctccc atggcgggaa catcggcgta
     1921 ctggtatccc ccggcaacgc tcgcggcgag aatcccgtcc tgttcttcca ggcgccgcgt
     1981 ctcttccacg atgggcgcca gcggctcggc attcgtgttc tggaagcgga tattgtagat
     2041 catgcccggc ttaaccagtg cctgcaccgg ctgccacttt ctggacacga tccccgacat
     2101 aatccaggcc gcacggattc cgcggtcttt ctggtcgatg tgcggattgg ttttgtagtt
     2161 cagcagggcc gtcgagagtt cgacaacctg gggtgaaatg ttggcatgaa agtcgtgcgt
     2221 taccacaatc gggaagccgc ttccgagcgc ctcccgcaaa cgccgcacga cttcgccatc
     2281 cgcatgaggg tagctttcgg cgaccattgc accatgaagc gcgagcagga ggccgtcgaa
     2341 cttgccgccg gactgaatgc gccggagcag atcgcccgtc aatgcgtcca gggcctcgtc
     2401 ggtcaccgga cccgagggcg tcgcgtgcgc tacgagcgta ggataaagat ccaggccgaa
     2461 acgcgctccc ccttcgataa agccgctgac ctcgtcattg ttcttgcccc attgctcgag
     2521 cgcagcgtcc ggatcgtcgc cggtgcgccg gtcgaaatcg gcaagagtgg tcttgacgga
     2581 gaagaacgag ttcgattcgt gaacgatgcc cccgatggcg attctcgggc gcggagtttg
     2641 tgcatgggtc gtcattttgt ggccgttgga ccggttctcg atctaccata ctacgaggta
     2701 tgcccggacg gagatatttg cgctggaacc gccaggagtt tgttggcatc cattgataga
     2761 ggaagccggc agcggcttgc cttctcaaac tccagcgtgg taagacatgg aaagcaatac
     2821 ggctgattgt actgggccca tgcaggacgg aagcaaccac tccgaaaaga cctgtgccgc
     2881 ggcaaaacct tccactgtcc aatctctatt ttcctgggcg cgcgacctgg ttctatccgt
     2941 cgtcatcgcc gccgtcgtca tcctgtttct ctatcagccg gtgaaagtcg agggcacgag
     3001 catgatgccg gccctcgaag accaggagcg catcttcatc aacaagttcg tctacagctt
     3061 cgggtggggc gaaatcgtgc gtggcgacat ggtcgtattc tggtatccgg ccgaccccag
     3121 caaa
//
LOCUS       DASTMK010000455         5488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_214726, whole genome shotgun sequence.
ACCESSION   DASTMK010000455 DASTMK010000000
VERSION     DASTMK010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214726"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VFQ79_19265"
     CDS             <1..887
                     /locus_tag="VFQ79_19265"
                     /inference="COORDINATES: protein motif:HMM:NF025695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi-alpha family protein"
                     /protein_id="HEU0141871.1"
                     /translation="PWEFESTFGRGDKTAHADRVRMGDYPPGATRIRNASPEDSGRWV
                     TLATMEPTSTELMAAATKASVDLAQKETESFLNAVLGEPLKEVGGLLKDRVSARRHRN
                     LVKIVLAAKECLSRAGVSPKEVPLKIIHPLLDAASLEEEPDLQDLWAKLLANCADPER
                     KEPVSTIFLNVLRELTVIEAQFLDHIVSRAIARSTQMRETKEHLRFEDLAEIGNLGAL
                     ATVFNERFSPSDNVFLYEIVEHFLRLGLLTAKVQFSKELSMVFSPNPVSKSIAMDLVE
                     YYYVTQLAFSFRKACQRS"
     gene            complement(1252..1437)
                     /locus_tag="VFQ79_19270"
     CDS             complement(1252..1437)
                     /locus_tag="VFQ79_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141872.1"
                     /translation="MDALAEMLDQRLRHWKPDTAAEARERITEVIELADQDLLDVARS
                     RAAEQEVLDLLDEPPAR"
     gene            complement(1774..3528)
                     /locus_tag="VFQ79_19275"
     CDS             complement(1774..3528)
                     /locus_tag="VFQ79_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141873.1"
                     /translation="MSGAYVNRRAQVPAGRHYYVWEVQDKRFCVLLNLDVVERISEML
                     EREAGPVAERAAELGGVLLGSAKELETGWQITIDGFEEIPFEHPRGIAYALVGPDRRK
                     LQQKLESRPVAGFFRAHARPGLFLDQDDFRLAQTFFPGPASVFLIVKRDSGGMNRGGF
                     FFWEDGDIQRRSSYLPFPFDRTWLESGKYQIRDVSPIAAPPVRSPRQIAPRGNRLPAF
                     TLPKVSWPKALHKPVVLTSFVAVSVFALLMTAAWFLSGERNAASRAHDIGLSATKNGE
                     NFRVTWNRNHPQVEEAARGVLWITDGRKEHRIDLDARQLKMGSVIYHPATSDVNFQLD
                     ILSEAPVSESVRAIAGGTGSAGTPDEAGTVASMPPGTVVEETTPPVPPVQPSGVSKQS
                     RKEVRRLDPPPGITDGADRMRTSRSSTPLELPPMLAVNMGRQLNQVQTPAVDQPREAP
                     PVVTVTMEPVEPSAARRFLQKIPGIRQLQRNAYRAGDEFEPAVPLKQPSPTLPPSLYS
                     TIHSTVPIDLRVSIDEQGRVFQTRLVSEEPLPPSLVRLAEDTAAKWSFRPARIKDKEV
                     DSEVVLQFRFSPALVSSR"
     gene            complement(3599..>5488)
                     /locus_tag="VFQ79_19280"
     CDS             complement(3599..>5488)
                     /locus_tag="VFQ79_19280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0141874.1"
                     /translation="VPVRGGTPRQITHEGSTNERPRWMPDSRRIAFLSNRGGSSQVWM
                     MDSDGANPRQVTSLATEAEGLLVSPDGKNILFVSAVYPDCPDLACNAARLEAEKASKV
                     KARTYTSLLYRHWASWKSGRTKHLMVIPVEGGTPKDLTPGNRDVPPFSLGGPDDYAIS
                     PDGTEVCYVTKTDPDLALSTNSDLFTVPIEGGEPVKITVSAGADNSPSYSPDGKYIAY
                     RAQERAGYESDRWRLIIMDRATGAATSLTETLDRSVTAFTWAPDSTRIFYTIEDRGRT
                     SVQMMSVTGGAARSIISGPAHVDDVQFADGGKIMVYSQNSGSRPVELYRVESSGGTAV
                     PLTRINDALLSQYGLPALGEMWVQSPDGARIHSFVVKPPGFRSDQKYPVLFLIHGGPQ
                     GAWGESWSYRWNPQVFAASGFLVVMPNPRGSTGYGQKFTDEINSDWGGKVYDDIMAVV
                     DQVATLPYANPQRIAAAGGSYGGYMVNWMLGHTQRFKAFVSHAGVFDLRSMTGETEEL
                     WFPLWEFRGMPWDNPDLYAKWSPSFFVQDFKTPTLVIHGEQDFRVPYGQGLQLFTALQ
                     MRQVPSKLLLYPDEGHWILKPQNTLLWYRTFLDWIGEWTRVPRPTAAPAKPVEGAAQP
                     APPQL"
BASE COUNT         1155 a         1595 c         1621 g         1117 t
ORIGIN      
        1 aaccctggga gttcgaaagc actttcgggc ggggtgacaa gaccgcccat gcggatcgtg
       61 tccgtatggg agattatcca cctggagcta ctcggattag gaatgcctcg ccggaagatt
      121 cgggccgatg ggttacacta gcgaccatgg agccgacctc gacggaactg atggcagccg
      181 cgacaaaggc gagcgttgat ctcgcacaga aggagacaga gtcttttctg aatgccgttc
      241 ttggtgagcc actcaaagaa gtcggcgggc tcctcaaaga tagagtgtct gcgcgccgac
      301 accggaacct cgtcaaaatc gtccttgcgg caaaagagtg tctttcgaga gctggagtga
      361 gcccgaagga agttcccttg aagattatcc accccttact agacgcagcg tctttagaag
      421 aggagccaga ccttcaagac ctgtgggcca agctgcttgc aaactgcgcg gatcccgaac
      481 gaaaggagcc cgtctcaacg atattcttga atgtgcttcg agaactcacc gttatcgagg
      541 cacagttctt ggatcacatc gtgagcagag ctatcgcccg ttctacccag atgcgtgaga
      601 cgaaagagca cctccgcttc gaagatcttg cggaaatcgg taatctgggc gcgcttgcca
      661 cagtcttcaa tgaacgcttc agtccgtccg acaacgtgtt tctatacgaa atcgttgagc
      721 acttcctgcg attaggtttg ctaacggcaa aagtccaatt ctcgaaagag cttagtatgg
      781 tgttctcacc aaacccagtt agcaaatcca tagcgatgga tttggttgaa tactactatg
      841 tcactcagtt ggcgttcagc ttccggaagg cttgtcagcg atcataatga gcatggccac
      901 gtgtccgtta ggcaagcacg ccgcagatca ggctcggctc attcggaagg cttttgaaga
      961 tcgagcgcaa aagccagcgc gtctttcagt cgcgccatca catcaacgga aagcctgcca
     1021 atcttgcgtt caaggcgcac ggttggtagg gatgccaatc cttgaacgtt ggctaccgat
     1081 tcacgatcaa ggaatggaag ccgtggcaac ggcacctcat atgggctgtt cctccgctga
     1141 gtcgtgagag gcacatacaa aacgagagat cgcggcgggt tggggtcctg acgcgagaca
     1201 ataacaactg gacgtgtttt ggcggcgagt cccagatctg ccagtcagag ctcagcgggc
     1261 agggggttca tcgagcaggt cgaggacctc ctgttcggcg gcgcgcgagc gtgcgacatc
     1321 aaggagatcc tgatcggcga gttctatcac ctcagtgatg cgctctctgg cctctgccgc
     1381 cgtatccggc ttccagtggc gcaagcgctg gtccagcatc tcggctaatg cgtccacgat
     1441 gacctcctgc tctcgttccc agtttaacgc cattgacttt ccagcatatt gccacaccgc
     1501 catgcgggac gtgcgcggtg aattagaggg tccgataata aacatgctca gcattgcagt
     1561 ttctgctttg gaacaaataa tgactaatcg ggcagtctcc ccagttcttg ggatcaggaa
     1621 gggcagtcgg cttgtatccc ccaattccgg ccgcgtaacg tccgttgccg atacatgccc
     1681 gctcaatgga tctccccttg gcgtacggga tttacagatg gtgcggccgc ccggccctat
     1741 tctcgtattt aactggaact gtaggcagct ggattagcgc gaactcacca gcgcgggaga
     1801 aaagcgaaac tgtaacacga cctcgctgtc cacttctttg tctttgatgc gagccgggcg
     1861 gaaagaccac ttcgcggcag tatcttccgc gagccgcacc agactcgggg gcaagggctc
     1921 ttcggaaacc agcctggttt gaaagacccg gccctgctcg tcaatagaaa cacgcaggtc
     1981 gatcggaaca gtggaatgaa tcgtactgta gagcgacggc ggaagcgtcg gactgggctg
     2041 tttcagcggt acggccggtt cgaactcgtc ccccgctctg taggcattac gttgaagctg
     2101 ccgaattccc ggaatctttt gcaggaatct gcgcgccgcc gaaggctcta ccggttccat
     2161 tgtcacggtg acgaccggcg gcgcttcccg aggctggtcc actgctggcg tctggacctg
     2221 attgagctga cggcccatat tcaccgcaag catggggggc aattccaggg gggtgctgga
     2281 acgagaagtt cgcatcctgt cggcaccatc cgtgattccg ggaggcggat cgagccgccg
     2341 aacctctttc cggctctgct tcgaaacgcc ggacggctga accggaggga ccggcggcgt
     2401 tgtttcttca acaactgtac ccggcggcat gctcgcaacc gtaccggcct cgtctggtgt
     2461 gccggcgctg ccggtgccgc cggcaatcgc acgaacggat tcggaaacag gggcctccga
     2521 tagaatatcc aactgaaagt tcacatcgct cgtggcggga tggtatatga cgctgcccat
     2581 tttcaactga cgggcgtcca ggtcgatccg gtgttccttg cgtccgtcgg tgatccatag
     2641 aacgccacgc gctgcttcct ccacctgtgg atgattccga ttccaggtca cccggaagtt
     2701 ctcgccgttt ttcgtcgcgg agaggccgat gtcgtgcgcg cgcgacgccg cattgcgttc
     2761 cccggagagg aaccaggcgg ccgtcataag aagggcgaat acggaaacgg cgacaaagga
     2821 agtcaggaca accggcttgt gtagcgcttt aggccaactg actttcggca aggtgaaggc
     2881 gggtagccgg ttccccctgg gagcgatttg ccgcggcgaa cgaacgggag gcgcggcaat
     2941 gggggaaacg tcgcggatct ggtatttccc cgattcgagc catgtgcggt caaacgggaa
     3001 aggaagatag ctggaacggc gctggatgtc accgtcttcc cagaaaaaaa acccgccgcg
     3061 gttcatcccg cccgaatccc gtttgacaat caggaagacc gaagccggtc cgggaaaaaa
     3121 ggtttgcgcc aggcggaaat cgtcctgatc gagaaacaac ccggggcgcg cgtgtgcgcg
     3181 gaagaacccg gccaccggcc gggattccag cttctgctgt agtttgcgcc ggtcgggtcc
     3241 gaccagtgca taagcgatgc cgcgcgggtg ctcaaacggg atctcttcga aaccgtcgat
     3301 ggtaatctgc cagcctgttt ccagctcttt cgccgaaccc agcagtacac cgccaagttc
     3361 cgcggcgcgc tcggctacag ggcccgcttc ccgctcgagc atctcgctga tgcgctccac
     3421 tacgtcaagg ttcagaagca cacagaatcg cttgtcctga acttcccaaa catagtagtg
     3481 ccggccagcg ggaacttgag cccggcgatt gacataggcc ccagacattt ttcctaaccc
     3541 tcaagcgggc tggtccatag tataacccga tacaccgtcg ggatgcggga aggcgctttt
     3601 agagctgtgg cggcgcgggt tgtgcggcgc cttcaaccgg cttcgcgggc gccgccgtgg
     3661 gtcttggcac acgggtccat tctccgatcc aatccaggaa agtgcggtac cagagcagcg
     3721 tgttctgcgg tttcaaaatc cagtggccct catcgggata caacaacagc ttcgaaggga
     3781 cctgccgcat ctgaagggcg gtaaacagtt gcaagccctg tccgtaggga acccggaaat
     3841 cctgctcgcc gtgaatgacg agcgtcggcg tcttgaagtc ttgaacaaaa aagctgggcg
     3901 accacttggc gtagagatcc gggttgtccc aaggcatacc ccggaattcc cagagcggaa
     3961 accacagctc ttcggtttcg ccggtcatac tgcgcaggtc gaacacgccc gcatgggaaa
     4021 caaatgcctt gaagcgttgc gtgtgcccga gcatccagtt caccatgtag ccgccgtaag
     4081 agccgccggc cgccgctatg cgctgcggat tcgcataggg cagagtggcg acctggtcca
     4141 ccacggccat gatatcgtcg taaactttgc cgccccaatc ggaattaatt tcatccgtga
     4201 acttctgtcc atacccggtt gatcctctgg ggtttggcat gacgacaaga aatccggaag
     4261 cggcgaagac ctgcggattc cagcgatagc tccaactctc tccccaggca ccctgcggtc
     4321 cgccatggat caaaaacaac acaggatatt tctggtccga tcgaaatccg ggcggcttca
     4381 ccacgaagct gtgaatcctc gcgccgtcgg ggctttgcac ccacatctct ccaagcgccg
     4441 gcaagccata ctgggaaagc agagcgtcgt tgatgcgcgt aagcggaacc gccgtaccgc
     4501 cgctcgattc tactctgtac agttcgacgg ggcgcgagcc actgttctgg gaatagacca
     4561 tgatcttgcc gccatcggcg aactgcacgt cgtcgacgtg tgcgggtccg ctaatgatgc
     4621 ttcgtgcggc gccaccggtg accgacatca tctgcaccga agtccgcccg cggtcctcga
     4681 tggtgtagaa gatgcgcgtg gagtcgggtg cccatgtaaa agcagtgacc gaacgatcga
     4741 gggtttccgt caggcttgta gctgcgccgg tggcgcggtc catgatgatc agacgccaac
     4801 ggtcgctttc atatccggca cgttcctgag cgcgataagc gatatacttc ccgtcgggtg
     4861 agtaagaggg tgaattatca gcgccggcgc tcaccgtgat tttcaccggc tcgccgccct
     4921 cgataggcac ggtaaagagg tctgaattcg tgctgagcgc aaggtccgga tcggtcttcg
     4981 tcacgtagca gacttccgtt ccgtcgggag agatggcgta atcgtccgga cccccgagcg
     5041 agaaaggcgg cacgtcgcgg ttgccgggag taagatcttt cggggtgcct ccctccacag
     5101 gaatcaccat caggtgctta gtgcgccccg acttccaact cgcccagtgc cggtacagca
     5161 gcgacgtata tgtgcgggct tttaccttgc tggccttctc cgcctccagg cgcgccgcat
     5221 tgcaagcaag gtcggggcag tccggataaa cggcgctgac aaacagaatg ttctttccgt
     5281 cgggcgaaac cagcagacct tccgcctccg tcgccagaga ggtgacctgt cgcgggttgg
     5341 ctccgtcaga atccatcatc cagacctgcg aggagccgcc gcggttcgac aggaacgcga
     5401 ttctgcggga atcgggcatc cagcggggtc gctcgttcgt ggacccctcg tgcgtgatct
     5461 ggcgtggggt tcctccacgt acgggtac
//
LOCUS       DASTMK010000456         6956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_343214, whole genome shotgun sequence.
ACCESSION   DASTMK010000456 DASTMK010000000
VERSION     DASTMK010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343214"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VFQ79_19285"
     CDS             <1..1035
                     /locus_tag="VFQ79_19285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0141875.1"
                     /translation="RIVVCWSVFTVVTGLVRSYFALIFARFVFGAAEAGAFPTVARAL
                     ARWFPAGERGWVTGIMWTGARLGGALAPPLATLLIAWTGWRATFVLFGSVGLVWSAVF
                     WIWYRDDPAQHRSVSPAELVYIRMHGSVSGPDVRSEPSIPTPWKRIFSSGTMWALFWM
                     YFATSYGFWFFLTWLPTYLIREHGVTVTRAGLLSAMPLALGAVACLAGGALSDWLTRR
                     TGSLRWGRRLVGLAGFTLTAAGFAAAAAAEGPLTAVVCLTLAAGAMDLAVPVAWAACL
                     DVGGRFGGTATGFMNTSSSISAMISPIAAAKLFETFGSFHAMFASAAVVYLLAGLLWL
                     KIDPSRPVDD"
     gene            1046..1861
                     /locus_tag="VFQ79_19290"
     CDS             1046..1861
                     /locus_tag="VFQ79_19290"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU0141876.1"
                     /translation="MARIPYLYQHYTWPELGEVAKRQPVVVLPIGSVEDHGPHLPVDT
                     DNFLIWSICEEAARRADGDILLLPPIPYGFETHHMDFHGTIDIRIEHLLNFVLDVTRS
                     VAHHGFERILIADGHGSNMPILDLVARRTNIETNALCGCFIWPSLATEEIRKVRESGR
                     GGMAHACELETSVYLHLDAGRVQFEKARKDMGMPPSSFIWMDLIEGSPVLLMDEWTRF
                     SKSGVYGDPTLATAEKGKIVFEAVVNAFVKLVREFKNRPRGERTDYHKQDSEF"
     gene            1885..2766
                     /locus_tag="VFQ79_19295"
     CDS             1885..2766
                     /locus_tag="VFQ79_19295"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0141877.1"
                     /translation="MNRILLLLIACAACASAAALAQTAPRVTRDISYAEPRNERQLLD
                     VYSPPTGSNFPVVVWVHGGGWMRGSKNEVEHKPAAFVEKGFVFVPVNYRFIPHVTMEV
                     IVRDVAKATGWVHANIARHGGDPNRIFLMGHSAGAQLAALLCTDSRYIEAEGVPRSSI
                     EGCVPVDGDTYDVPLQVATAAARRKSLKQPPPKMGHTEKFGSLEQQRELSAVNHVAPN
                     RGIPPFLILHVADHTDTTAQAHRLWAALDQAGIPARLFGAEGTDHVKLDRDLGLAGDP
                     ATKVLFEFTDGVLKPKP"
     gene            2825..3154
                     /locus_tag="VFQ79_19300"
     CDS             2825..3154
                     /locus_tag="VFQ79_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0141878.1"
                     /translation="MSKPTDLVQGTLDLLILKTLALEPMHGWAIAHRIHQMSSEVLQV
                     GQSALYPALHKLEHQGWVKSEWKISETNRRAKYYSLTRAGRKALSEEAANWERLSAAI
                     SAIVRTA"
     gene            3169..5790
                     /locus_tag="VFQ79_19305"
     CDS             3169..5790
                     /locus_tag="VFQ79_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0141879.1"
                     /translation="MPRWFTQLRLIARSIFRKSSLDRELDEELQYHLQRELDVRMNAG
                     LTPEEAHFAAWRAMGAMAQSMEECRHVRRVNLVDDFLQDIRYAAFSLRRNPLVSALIV
                     LIMALGIGANTAVFGVINAVLLKPLPYSGADRIVTLSTRDFVTGDINPLVTIANYRDW
                     RDQNSSFEAMATYRGGEYPVTSGSVAEYTQTVTVDIAFFRVFGVEPVIGRTFIPEEMV
                     PGSQAVVISYAYWQTRLGGDPRVLERTVRVGNDPRPIVGVMPAGFRFPRQTDIWAPER
                     TRSTSRTGHNLFAVGRLKPGVSLERAQADLNTIAARLEQQYPASNKGRGVVAAPLQDE
                     LVRDVRLTLYLLWGVVAVVLLIACANTATLLLGKAMARTREIAVRTALGASRSRIIRQ
                     LVTESLLLALIAGAAGIVLAYWGVYLLAALTPSEAVRLTEIRIDGVVLSFTMAVSIAA
                     TVLFGLVPALHASKVDLTGVIKQAGRSMHGRRMVRTRGMLVVSEIALSVVLVTGAGLL
                     IKSLIALHNVELGFKPGNVLVMKATGVHDRKENDAFFRSTMSRIASLPGVVAVGATSI
                     PPGDLSNAGSGSYFIDWLPEQRERTVNPEALFTIVAPGIFEALGIPLKSGRDFNERDT
                     GDSPLVAIVNEALVRKSFGNEDPIGRTIFCTFDRKDPMTIVGVVGDVRQRNPALAPMP
                     ECYMPYTQHSYNNATLYIVTRTAGDPSPLARPVLRIAAEISPNAAASVTTMEAEVSKK
                     ADAPRFRALLFAVLAVFAVCLAMAGVYGVIAYAVGQRTSEIGLRIALGASPGSVLQLV
                     LGQGLVLAGIGLALGLAAAIASTRLLTTVLFQVQPHDIPVYVSVAILLALVTLLASYV
                     PARRASRIDPMAALRQE"
     gene            5883..>6956
                     /locus_tag="VFQ79_19310"
     CDS             5883..>6956
                     /locus_tag="VFQ79_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141880.1"
                     /translation="MSIGRWHRVVLLIAFGGCAAIPLKLLGDLEGSYVLPLDHAAIQY
                     ATAALDDPVTRLKRRISEGKVSLSFEPDHGYLPALLKELDVPVSSQVLVFSKTSFQAP
                     RISPRTPRALYFNDEVSLGWVRGGDVLEIAATDPRQGTIFYTIDQEEASKPRIERRGE
                     CLQCHASPGTVGVPGLVVRSIYPEPSGMPLFHAGSFVTDHRSPLNQRWGGWYVTGTHG
                     SQRHMGNIFVMDRDHPDKLDMDAGANVTGLTGRFDTGAFLSPHSDIITLMVLEHQTRM
                     ISLITRLAFETRMALHDQKAINQALGRPAEELTDSTKRRIGNVAEALVKYMLFVEETA
                     LEDPIKGTSGFAAEFAKKGPRDSS"
BASE COUNT         1302 a         2072 c         2150 g         1432 t
ORIGIN      
        1 cgcatcgtgg tctgctggtc tgtattcacc gtcgtcacgg gactggtacg gagctacttc
       61 gcgctgatct tcgctcgctt cgtctttgga gcggccgagg cgggcgcgtt tccgacggtg
      121 gccagggctc tcgcccgctg gtttcccgca ggtgagcggg gatgggtgac gggcatcatg
      181 tggacaggcg cccgcctcgg cggcgctctc gcacccccgt tggctacgct gctgattgca
      241 tggaccggtt ggcgcgccac cttcgttctg ttcggatccg tgggcctggt gtggtcggct
      301 gtgttttgga tctggtaccg cgacgacccc gctcaacacc ggagcgtcag tcccgccgaa
      361 ctcgtctaca tacgaatgca tggatcggta agcggtcccg atgtgcgctc cgaacccagc
      421 atccccacgc catggaagcg cattttcagc agcggaacga tgtgggcgct cttctggatg
      481 tacttcgcta cctcgtatgg cttttggttt ttcctcacgt ggctgccgac gtacctgata
      541 cgcgaacatg gcgttacggt aaccagagcg gggctcctct ccgcgatgcc gctcgcgctc
      601 ggcgccgtgg catgcctcgc cggaggtgcg ctatcggatt ggctgacgcg ccgcacgggg
      661 agcctgcggt ggggtcgaag gttggtaggc ctggccgggt tcactttgac agccgccgga
      721 ttcgccgctg cggcggcggc cgagggaccg ctgaccgcgg tggtctgcct gactctggcg
      781 gcaggcgcca tggacttggc cgtccccgtc gcctgggccg cgtgcctcga cgtgggcggg
      841 cgcttcggag gcaccgcgac aggcttcatg aacaccagtt ccagcatctc tgcaatgatt
      901 tcgccgatcg ctgcggccaa gctgttcgag acgtttggct catttcacgc gatgtttgcg
      961 agtgccgccg tggtatacct gctggcgggt cttctttggt tgaaaatcga tccaagccgc
     1021 ccggtggacg attaaggatg aatatatggc tcgtatcccg tatttatatc agcactacac
     1081 atggcccgaa ctgggcgaag tagcgaagcg gcagccggtg gtggtgctgc ccattggttc
     1141 cgtggaggac catggtcctc atctgcccgt ggacacggat aatttcctga tctggtccat
     1201 ttgcgaggag gctgccaggc gtgccgatgg cgacatattg ctgctgcctc ccatccccta
     1261 tggtttcgaa acgcaccaca tggactttca tggaaccatc gacatccgca tcgaacacct
     1321 gctgaatttc gtgctcgatg tcacccggag cgtggcgcac catggtttcg agcggattct
     1381 gatcgccgat ggccatggct cgaacatgcc gatcctggac ctggttgcgc ggcggaccaa
     1441 tatcgaaacc aacgcgctgt gcggttgctt catctggccg tcgctcgcca cggaggagat
     1501 ccggaaagtg cgtgaatcgg gcaggggcgg aatggcccac gcctgcgaac tcgagacttc
     1561 tgtctacctg cacctcgacg ccggccgcgt gcaattcgaa aaggcgcgca aggacatggg
     1621 catgccgcct tcgtcgttca tttggatgga cctgatcgag ggctcgccgg ttctgctgat
     1681 ggatgaatgg acgcgtttca gcaaaagcgg tgtctacggc gatccaacac tggcgacagc
     1741 ggaaaaaggc aaaatcgtct tcgaggcggt ggtgaacgcc ttcgtgaaac tcgttcgcga
     1801 attcaagaac cggccgcggg gggagcggac ggactatcac aagcaggact ctgaattcta
     1861 aataccatcg aaagggtgat ctccatgaac cggatcttgt tgttgctgat tgcctgcgcc
     1921 gcctgcgctt ccgccgcggc gcttgcccag acggcacctc gcgtgacgcg cgacatcagc
     1981 tatgccgaac cccgaaatga gcgccaactg ctcgatgtgt attcaccgcc aaccggatcg
     2041 aacttcccgg ttgttgtgtg ggttcacggc ggcggctgga tgcgtggcag caagaacgag
     2101 gtggaacaca agccggcggc atttgtggaa aagggcttcg tcttcgtgcc ggtgaactac
     2161 cggtttatcc cccacgtgac catggaggtg atcgtgcgcg atgtggcgaa ggctaccgga
     2221 tgggtacacg ccaacatcgc gcgccacggc ggcgatccga accggatttt tctgatgggt
     2281 cattccgccg gtgcgcaact cgccgccctg ctttgcacag acagccgcta catcgaagcg
     2341 gagggtgtgc cgcgttcaag tattgaagga tgtgtacctg tggacggcga tacctacgac
     2401 gttccgctac aggtggcgac ggcggcggcc cgccgcaaga gcctgaagca gccgccgcca
     2461 aagatgggcc acacggaaaa gttcggcagt ctcgaacagc aacgcgagtt gtcggcggtg
     2521 aatcatgtcg cgccgaacag aggcatccca cccttcctga tcctccacgt ggccgatcac
     2581 acggacacta cggcacaagc ccaccggctg tgggcggcgc ttgaccaggc tggcattccc
     2641 gccaggctgt ttggagccga aggaaccgat cacgtgaaac tcgatcgcga tctcggcctc
     2701 gccggcgatc cggcgaccaa ggtgctgttc gaattcacag atggcgtgct gaaaccgaag
     2761 ccataatact cttgtattga gcgcacggct gtggcatgct gatgtcagta actgaggtat
     2821 agccgtgagc aaaccgaccg atctcgtgca gggcaccctg gacctgctta tcctcaaaac
     2881 ccttgccctc gagccaatgc atggctgggc cattgcgcat cggatccacc agatgtcgag
     2941 cgaagtcctg caggttggac agagtgcgct ttaccccgca ctgcacaaat tggagcacca
     3001 aggctgggtc aagtccgagt ggaagatctc ggagactaac cgtcgcgcca aatactactc
     3061 actcacgcgc gccgggcgca aagctctcag cgaggaagca gccaactggg aacggctctc
     3121 ggcggccata tcggcgatcg tgcgcacggc ctgaacgagg aggcggagat gccacgctgg
     3181 ttcacacagc ttcgtttaat cgctcgttcc atcttccgta agagcagcct cgaccgggag
     3241 cttgatgaag aacttcagta ccacctgcaa cgcgagctcg acgtgcgaat gaatgccggt
     3301 ctcacaccgg aagaagcgca cttcgctgcg tggcgtgcga tgggcgcaat ggcccaaagc
     3361 atggaggagt gccgtcacgt acgtcgcgta aatcttgtcg acgacttctt gcaggatatc
     3421 cgctacgcgg cattctcgct gcgcaggaat ccgctggttt ccgctctgat cgtacttatc
     3481 atggctctcg gcattggcgc gaacactgct gtgttcggcg taattaacgc cgtgctcctc
     3541 aaaccgttgc cgtattccgg ggcggatcgg attgtgacgc tctccaccag ggatttcgta
     3601 acaggcgaca tcaatcctct cgtgacaatc gccaactatc gggactggcg cgatcaaaac
     3661 tcctcgttcg aggcgatggc aacctaccgc ggtggagagt atccggtaac atccggcagc
     3721 gtggcggagt atacgcagac cgtcactgtc gatatcgcat tcttccgtgt ctttggtgtc
     3781 gagccggtta tcggccgcac cttcattccg gaggagatgg tcccggggag ccaggcggtg
     3841 gtgatcagct atgcgtattg gcagacccgc ctgggaggag atccgcgcgt actggagagg
     3901 accgttcgcg tgggtaacga ccctcggccc atcgtagggg tgatgcccgc gggtttccgc
     3961 tttccacggc agacggacat ctgggccccc gagaggacgc gttcgaccag ccgcacggga
     4021 cataatctct tcgcggtcgg gaggctcaag ccgggtgttt ccctggaacg tgcgcaagcc
     4081 gacttgaaca caatcgccgc gcgcctcgag cagcagtacc cggcgagcaa caaaggacgc
     4141 ggcgttgtcg cagcgcctct ccaggacgag cttgtgcgtg atgtgcgtct cacgctgtac
     4201 ctgttgtggg gcgtggtcgc cgtagtgctg ctgatcgcgt gcgccaacac tgccacgctg
     4261 ctgctgggca aagcgatggc gcgcactcgt gaaattgcgg tccggactgc gctcggagcc
     4321 agccgctctc gaatcatccg gcaactggtg acggagagcc ttctgctcgc gcttattgcc
     4381 ggcgccgccg gcatcgtgct ggcctactgg ggcgtgtatc tgctggcggc actaacaccg
     4441 tccgaggccg tccggctcac cgaaatcagg atcgatggag tcgtactctc attcacgatg
     4501 gccgtttcga tcgcggcgac tgtgctgttc ggcttggttc ccgcactcca cgcgtccaag
     4561 gttgatttga ccggcgtgat taagcaggca gggcgatcga tgcacgggag gcgaatggtg
     4621 cgtacgcgcg gcatgctggt cgtctccgag atcgcgctat cggttgtgct ggtgaccggc
     4681 gcaggtctgc tgatcaaaag cctgatcgcc ttgcacaatg tcgagttagg tttcaagccc
     4741 gggaatgtac tggtgatgaa ggcgaccggc gttcatgacc ggaaggagaa tgatgccttt
     4801 ttccgaagca ccatgtcacg aatcgcatcg ctcccgggcg tggtcgccgt cggcgcaacg
     4861 tcaataccgc cgggagattt atccaatgcc ggctccgggt cctatttcat cgactggctc
     4921 ccggagcaaa gggagcgcac ggtgaatccg gaagctcttt ttacaatcgt cgcacccggc
     4981 atatttgagg cgttggggat tccgctgaag agcgggcgcg acttcaacga aagggacacg
     5041 ggagacagtc ccctggttgc catcgtcaac gaggcattgg tccggaaatc gttcggcaat
     5101 gaagacccga tcggcagaac gatcttctgc actttcgatc gaaaagatcc gatgacaatc
     5161 gtcggtgttg ttggcgatgt gcgccagcgg aacccggctc ttgctcctat gccggagtgc
     5221 tacatgccat acacgcagca ctcgtacaac aacgcaaccc tctacatcgt tacccgaacg
     5281 gcaggcgatc cgtctccgct cgcccgcccg gtgcttcgta tagcggcgga gatctctccg
     5341 aatgcggcgg cgtccgttac gacgatggaa gcggaagttt cgaagaaagc agacgctccg
     5401 cggttccgcg ctctgctctt tgccgtgctc gccgtttttg cagtgtgcct tgccatggcg
     5461 ggtgtatacg gcgtgattgc ttatgcggtg ggccagcgga caagcgaaat cggcctgcgg
     5521 atcgcgctcg gcgccagtcc gggctccgtg ctgcaactcg ttctcggcca aggactggtt
     5581 ctggcgggca tcggtctggc tttgggctta gcggccgcca tcgcctcaac gcgattgctt
     5641 acgaccgtgc tgtttcaagt gcagccgcac gatatcccgg tctatgtgtc tgtcgcgatt
     5701 ctgctcgccc tcgtgactct gttggccagt tacgttccgg caaggcgtgc atccagaatc
     5761 gatccgatgg ccgcactccg tcaggaatag acctgccgga atcgccgcgt ctcgttactg
     5821 atgtaaacta ccagaagcag cgacgatctg ggtccagcta atacaatttc ggagagcatt
     5881 cgttgagtat cggtcgatgg caccgtgtgg ttctgctgat cgcgtttggc gggtgcgcgg
     5941 cgattccctt gaagcttctc ggcgacctgg aagggtccta tgtcctgccg ctcgatcatg
     6001 cggccatcca atacgcgacg gcggcgttgg acgaccccgt cacgcgattg aagcgccgca
     6061 tcagtgaagg caaagtgagc ttgtctttcg aaccggatca tggctatctg ccagctctgc
     6121 tcaaagaact cgacgtgccg gtttcctccc aggttctcgt gttctccaaa acaagtttcc
     6181 aggcgccgcg catctctccg cgcacgccgc gagcccttta cttcaacgat gaggtgtcac
     6241 tcggctgggt gcgcggcggc gacgtcctgg agatcgcggc cacggacccc aggcagggca
     6301 cgatcttcta cacgatcgat caggaagagg cgtcgaagcc gcgcatagaa aggcgcggcg
     6361 agtgcctgca atgccacgct tcgccaggaa cggtgggagt gcccggactg gttgtccgct
     6421 ccatttatcc cgaaccttcc ggcatgcctt tatttcacgc cggatcgttc gtgacggacc
     6481 atcgcagtcc gctcaaccag cgctggggag gatggtacgt gaccggtacc cacgggtcgc
     6541 agcggcacat gggcaatatc ttcgtcatgg accgggatca ccccgacaag ctggatatgg
     6601 acgccggcgc gaatgtcacc ggcctcaccg ggcggtttga caccggcgct tttctcagcc
     6661 ctcatagcga cattatcacc ctgatggttc tggagcatca gacccggatg atcagcctca
     6721 tcacgcgtct cgccttcgaa acacgcatgg cgctgcacga tcagaaagcg atcaaccagg
     6781 cgctgggccg gcctgccgaa gaactgacgg attcgacgaa acggcgtatt ggaaacgtcg
     6841 ccgaggcgct ggtgaaatat atgttgttcg tcgaagaaac ggcgcttgaa gatcccatca
     6901 aagggacctc cggtttcgcg gccgagtttg cgaaaaaagg acctcgcgat tcgagc
//
LOCUS       DASTMK010000457         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_375233, whole genome shotgun sequence.
ACCESSION   DASTMK010000457 DASTMK010000000
VERSION     DASTMK010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375233"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="pdxT"
                     /locus_tag="VFQ79_19315"
     CDS             complement(<1..514)
                     /gene="pdxT"
                     /locus_tag="VFQ79_19315"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU0141881.1"
                     /translation="MRRVGVLALQGDFEAHQKALQQAGAEAIPVRTAEQLEQVEGLII
                     PGGESTTMLKLLRVENMLEPLKRFAEVKPVFGTCAGAILLASKVTNPAQESLNVVDID
                     VERNAYGRQLDSCVAHIDAAPGFAREAGAPEDFEAVFIRAPVIRRVGDGVKVLARFQG
                     NPVLVEQGRHL"
     gene            complement(519..1487)
                     /locus_tag="VFQ79_19320"
     CDS             complement(519..1487)
                     /locus_tag="VFQ79_19320"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HEU0141882.1"
                     /translation="MLGKKTFTISSILLATAGFAAAQPDYFPLQPGNQWIYRSSGPTG
                     SEIRTVEVVRTATMAAGEYSLVRGLWEGDLWLRMTPERKLIAFGEDQVERLYADFSAG
                     DGGTYQEDVPPCLQTAHVVSHSAEYSGPIGEFSNALEIRYDPGNCADAGNQEDKYLPG
                     IGLVSRRETTFAGPRQFDLIYARIGDVTVISGPGTGFGLTIDRTTYPASGERDSPEMI
                     ARLTLRNGQEQPLSVTFPTGQRFDLQIWNDRGESVYTWSADKLFPQVMETIVIGPHEE
                     KNWVVDVPLAALPEGRYAVKVWLTSSEPPVFSAALSFEIRPSEPAQ"
     gene            complement(1459..2427)
                     /locus_tag="VFQ79_19325"
     CDS             complement(1459..2427)
                     /locus_tag="VFQ79_19325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0141883.1"
                     /translation="MRYRKLGRTGIEVSEIGYGAWGIGGALWQGGNDDESIQALRMAI
                     DRGVNLIDTALAYGTGHSEKLVGQVVKESGKEIFIATKVPPKNQLWPARPGIGINEVF
                     PYDYVMKCTETSLRNLGIETVDLQQLHVWNPEWIGRDEWKRAFEDIKRQGKARWVGVS
                     INDHQPDSALELIETGLIDTVQVIYNIFDQSPEKHLFPLCLERNIGVLARVPLDEGSL
                     TGTISEKSEFAPGDFRASYFGGDRKRQVVERVNALKADLAGVDGELPEIALRFCLSHE
                     AVSTVIPGMRRVRNVERNIAVSDIGPLDRNTLEKVKAHAWEKNFYD"
     gene            2467..3243
                     /locus_tag="VFQ79_19330"
     CDS             2467..3243
                     /locus_tag="VFQ79_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141884.1"
                     /translation="MKRLFIVLVLTAAAALTVSARNYKLYMKDGEYHVVREHKVEGDR
                     VRFYSVERGEWEEIPVALADLDRTSKEIAEEESARKEETAILAAEDKAEREMRREIER
                     IPQEPGVYYDEGETVRAIKQAESKAVTNKGRSILKVLAPVPVITGKATVEVDGARSAN
                     VVNKSRPEFYFRLAELERFAMVRATPKKDSRVIVKWTIVPVTNEIVDEFEEVEMFRRQ
                     VAEGIYKVWPEQPLKPGEYAIIEYTEGKGNVQIWDFAYRP"
     gene            complement(3244..4350)
                     /locus_tag="VFQ79_19335"
     CDS             complement(3244..4350)
                     /locus_tag="VFQ79_19335"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0141885.1"
                     /translation="MRILIDASSLLLRSAGVKTYFYHWITHLRQQAGADRIFAFPLLK
                     ELGELHHDRSVLRPWQTWPRLGLVYAAKFGWSPVVDLLTRNADVFHISNLVRNPPRRP
                     LVTATIHDLTYALMPDVHTPANVEADTGYAERVLKKARKLIAVSENTRRDAVRVLRIS
                     PNRVSVIYPGVAPAFFNVDATRVAGVRQKFGIEKDYVLFVGTIEPRKNVGGLLDAWES
                     LADELRHEFRLVIAGPKGWGSESVTARLEKGVKGVRYAGYVPEQDLPALTAGARVLAY
                     PSLYEGFGFPVAQAMAAGVPVITSNVSSLPEVTGPGGILIDPRSPVELASGLKNILLS
                     PSLREKLAGEAKRQALRYQWDRCAQESLEFFREL"
     gene            complement(4379..4708)
                     /locus_tag="VFQ79_19340"
     CDS             complement(4379..4708)
                     /locus_tag="VFQ79_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141886.1"
                     /translation="MKTLTTLIFSAALFLASANQNTKTFVGVITDTMCGDNHVHLNVA
                     PDEKCVRECVKSSSKYKYALHDGKRMYTLSDQQTPEKFAAQKVKVTGVLYEKTGIIKV
                     EKIEAVK"
BASE COUNT          996 a         1429 c         1307 g         1043 t
ORIGIN      
        1 caaggtgccg gccctgttcc accaggacgg gattgccctg gaagcgggcc agaactttca
       61 cgccgtcgcc gactctccgg atgaccgggg cgcgaatgaa gacagcttcg aagtcctccg
      121 gcgctccggc ttcccgtgca aagccggggg cggcatcgat gtgagcaacg caactgtcga
      181 gttgccggcc gtacgcgtta cgctcgacat cgatgtccac cacgttcagc gattcctgtg
      241 ccgggttggt taccttggag gccaaaagaa tcgcccccgc gcacgtgccg aacacaggct
      301 tcacttccgc aaagcgcttc agcggttcca acatattttc cacgcgaagc agtttcagca
      361 tggttgtgct ttcaccgccc ggtataatca gcccttccac ctgttccaat tgttccgccg
      421 tacgcaccgg tatcgcttcg gcaccggcct gctgaagcgc tttttgatga gcctcgaaat
      481 cgccttgcag cgcgaggact ccgacccttc tcatgccttc attgtgcggg ttcagaaggc
      541 cgaatttcaa agctgagcgc tgccgagaac actggtggtt cgctgctggt gagccacacc
      601 ttcaccgcat accggccttc gggcaacgcc gcgaggggaa cgtcgactac ccagtttttc
      661 tcctcgtgcg gcccgatgac aatggtctcc atcacttgcg gaaacaattt gtcggcggac
      721 caggtgtaga cgctctcacc gcgatcgttc cagatctgca gatcgaatcg ttgaccggtc
      781 ggaaacgtaa cgctcagcgg ctgctcctga ccgttccgca gcgtcaggcg tgcgatcatt
      841 tccggcgaat ctctctcgcc ggaagcaggg tacgtcgtcc ggtcgatcgt caatccgaag
      901 ccggttcccg gccctgagat cacagttacg tccccgatgc gcgcatagat caaatcgaac
      961 tgccgcggtc ccgcaaacgt cgtctcacgg cggcttacca ggccgattcc aggcagatat
     1021 ttatcttcct ggttgccggc atcggcacag ttgcctggat catatcggat ttccagagca
     1081 ttggaaaact ctccgatagg tccggagtac tcggccgagt gtgagacaac atgcgcggtc
     1141 tgaaggcacg gcggaacatc ttcctggtac gtgccgccgt ctccggccga gaaatccgcg
     1201 tacagtcttt ctacctggtc ttcgccgaat gctataagtt tccgctcagg cgtcattcgc
     1261 aaccagagat cgccttccca aagcccgcga acgagtgagt actcgccggc ggccattgtg
     1321 gccgtacgaa ccacctccac ggttcggatt tcgctcccag tgggtccgct ggaccggtag
     1381 atccattggt tacccggttg caacggaaaa taatccggct gcgcggcggc aaatcccgcc
     1441 gtggccagca gaattgagct aatcgtaaaa gtttttttcc caagcatgtg cttttacttt
     1501 ttcgagtgta tttcggtcta acgggcctat atcggagacg gcgatattgc gttcaacgtt
     1561 tcgaacgcgt cgcatcccgg gtatgaccgt tgagaccgcc tcatgcgaga gacagaagcg
     1621 aagggcaatc tccggcagtt cgccgtcgac cccagcaagg tctgctttca aagcgtttac
     1681 gcgctccacc acctgcctct tgcgatctcc tccaaaataa gacgcgcgaa aatcgcccgg
     1741 cgcgaactca cttttttcgg aaattgtacc ggtcaggctg ccttcatcca atggaactct
     1801 cgccagcact ccgatattcc gttctaaaca aagtggaaac aaatgctttt caggactctg
     1861 gtcaaaaatg ttgtagatca cctgtaccgt gtcgatcagt cccgtctcga tcagttcgag
     1921 cgcggaatca ggttgatgat cgttgatgga gacgcccacc catcgtgcct tgccctgccg
     1981 cttgatgtcc tcgaaggctc ttttccattc gtcccggccg atccactccg gattccagac
     2041 atgcaattgc tgtaggtcga cggtctctat cccgagattg cgcaggctgg tttcggtgca
     2101 tttcataacg tagtcatatg gaaatacttc gtttataccg atgcccgggc gtgccggcca
     2161 caattggttt ttgggtggta ctttggtggc tatgaaaatt tcttttccgg attctttcac
     2221 aacctggcca accagcttct cgctgtgtcc ggtgccatag gccagggcgg tgtcgatcag
     2281 gttcacgccc cggtcaatcg ccatgcgcag cgcctgaata gattcgtcgt cgttgccgcc
     2341 ctgccacagc gcgcctccga ttccccatgc tccgtagccg atctcgctta cttcgatgcc
     2401 tgttctgccg agttttcgat agcgcatgaa cccattgtat tcagtgcgaa aataaaagaa
     2461 gagcaaatga aaaggttgtt catcgtcctg gtgctcaccg cggccgccgc tctgaccgtt
     2521 tccgcgcgta attacaagct ctacatgaag gatggcgaat atcacgttgt ccgcgaacac
     2581 aaggtggagg gagatcgcgt ccggttttat agcgtggaac gcggcgagtg ggaggagatt
     2641 ccggtagcgc tcgccgatct cgaccggacc agcaaggaga tcgccgaaga agagagcgcg
     2701 cggaaagagg aaacggctat tctcgcggcg gaggataaag ctgaacgcga gatgcgccgg
     2761 gagatcgaac gcattcccca ggaacccggc gtctactacg acgagggcga aactgttcgc
     2821 gccatcaaac aggccgaatc gaaagcggtc acgaacaagg gaagaagcat tctgaaagtg
     2881 ctggctcctg tcccggtgat tacggggaag gccaccgttg aagtggacgg cgcgcgctcg
     2941 gcgaatgtcg taaataagtc caggcccgag ttttatttca ggttggccga gttggagcgt
     3001 ttcgccatgg ttcgggccac ccccaagaag gactcgcgcg tgatcgtcaa gtggaccatt
     3061 gttccggtga cgaacgagat tgtcgacgag tttgaggagg tggagatgtt tcgccgccaa
     3121 gttgccgagg gtatttacaa ggtttggccc gagcaaccac ttaaaccggg cgagtacgca
     3181 attatcgaat acaccgaggg caagggcaac gtgcagatct gggactttgc ttatcgcccc
     3241 tgatcaaagt tccctgaaga actccagaga ctcttgtgca caccggtccc actggtatcg
     3301 taatgcctga cgcttggcct cgcctgccag tttctctcgc agagacggcg acaatagaat
     3361 attctttagc cccgatgcca gctcaaccgg actgcgcggg tcaatcagga tcccgcccgg
     3421 accggtcact tccggtaaag acgagacatt ggaagtgatg accggcacgc cagccgccat
     3481 ggcctgcgcc accggaaacc cgaagccctc gtaaagggac ggatacgcca agacgcgcgc
     3541 acctgccgtc aacgcaggca ggtcctgttc cggcacgtaa cctgcatagc ggaccccctt
     3601 cacccccttt tcgaggcgcg ccgtcacgga ttcagagccc catcctttcg gcccggcaat
     3661 cacgagccgg aactcgtgcc ggagttcgtc cgcgagactc tcccaggcat ccaacagccc
     3721 gcccacgttt tttctgggct cgatggttcc cacaaacaac acgtagtcct tttcgatgcc
     3781 gaacttttgc cggacgccgg caacgcgcgt ggcatccaca ttaaagaaag caggcgcaac
     3841 gcccggataa atcacagaca cccggttcgg ggaaatccgc agcacgcgaa cagcatcgcg
     3901 gcgtgtgttc tccgacacgg caatgagctt tcgcgctttc ttcagcacgc gttccgcata
     3961 tcccgtatcg gcttccacat tggcgggcgt atgaacgtcc ggcatgagtg catacgtgag
     4021 gtcgtggatg gtcgccgtca ccagcgggcg ccgcggcgga ttgcgcacca ggttcgaaat
     4081 gtgaaacaca tcggcgttcc tggtgaggag atccacgacg gggctccacc cgaattttgc
     4141 tgcatagaca agcccgagcc tcggccaggt ctgccagggg cgtaaaaccg agcggtcgtg
     4201 atgcagttcg cccagctcct tcaataaagg aaaggcgaaa atacgatcgg cgccagcctg
     4261 ctgccgcaag tgtgtgatcc agtgataaaa ataggtcttc acaccggcac tacgcagcag
     4321 cagggaactg gcgtcgatca ggattcgcat cagagattgc gcacgcctgg ccggcgcgct
     4381 atttcaccgc ttcaatcttt tccaccttga tgataccggt cttctcgtac agcacgccag
     4441 taactttcac tttttgcgcc gcgaacttct ccggcgtctg ctgatcgctg agcgtgtaca
     4501 tcctcttgcc gtcgtgcagg gcgtacttat acttggagct tgacttcacg cactcccgca
     4561 cgcatttctc gtcgggtgcc acgttcaggt gcacatggtt gtcgccgcac atggtgtcgg
     4621 taatcacacc cacgaaagtc tttgtattct ggttcgcgga cgccaggaac agcgccgctg
     4681 aaaagattag agttgttaac gttttcattc cgcctccaaa agtcttatcc ctttctgttg
     4741 atgcagccgc cgccagagcg tgtcgtaacg ctctt
//
LOCUS       DASTMK010000458         3622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_566166, whole genome shotgun sequence.
ACCESSION   DASTMK010000458 DASTMK010000000
VERSION     DASTMK010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566166"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VFQ79_19345"
     CDS             <1..1212
                     /locus_tag="VFQ79_19345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0141887.1"
                     /translation="RNQALQMLTSVGVHDQVALYVLDNGLRIVHEFTADTVALARKIA
                     AEQEQVVDAPDPEVNPLTDASVGSLPFSDIGPGGLSTTKREERFFIERRVTGTLKAFE
                     MIAAHLAAIPGRKSLIWVSAGFPPSLGFDLPPVGKRPEGFADARTFTPELNRALSRLN
                     EADVSVYPVDARGLSPNPGVWKNIATMQELAERTGGKAYYNRNDLAQAMRSALDDVRV
                     SYTLAYYLPMAATRRDTLHRIRIRIHRPGVTVRHRREYLERAGGAAIAPAEAKSEARQ
                     AWSSPLNSTALRIDAKPVRSGNTLVIQIGLDASGISLTRKDGRLTGELLILTGFRKDD
                     GTPLEIADSKSVALALREQTYQKVLQEGLKARVQLPVPGNAASVRLLVRDTATNKLGT
                     LTIPLQAIPET"
     gene            complement(1290..3011)
                     /locus_tag="VFQ79_19350"
     CDS             complement(1290..3011)
                     /locus_tag="VFQ79_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141888.1"
                     /translation="MNKSLLIAALLVAGCLAATEHPTVSGPLVVSERWPQATDLVTWT
                     KDVMRLAGVEKASETAQGKAFFEWLRLYCRMAVGGMIQAHEGDFGKERYVLDAHKSLF
                     VYGWGYCDTCSRAADAAWQEYKGDAKAAERVVVQNESGGFHTMYRLRLDGNHGAFDPR
                     YGYYLIDRDAPDARILDWAEVGNDENILRNRSFKHRSRPFFEYFAQEWDRALALQPAY
                     FENQRAWEKAGSPKRSVFGDSHYKMGTRFHDMDFRLPKGMTIERFWDNSARKFYRPVS
                     ESANRELPFRPSGRFYRVTETMLDGNWPKYDPNYERAKPYLATIPKNENYDPEMAGGR
                     SIGQAWGRMTYAPALAQEDLKDVLAPGSSLMHSAAAPYLRPREAAAGGEAVLDFYSPY
                     VLVDGTLNAELAGVEEGDASIALRTLRAKTNNAQDPDVWSEWQTLPSRPGKMSIELGR
                     PRFNGRDVSIHGVYRFQVRLSVKPRPGRQSMPGLNALAIDLYFENGIMSLPQIFAGRN
                     TIHFKLQDAAALRAPVVVTYRYQTAGGEKAHEQVLKPADFKGGSATYALDAPGLVRCN
                     SIAISYR"
     gene            3072..>3622
                     /locus_tag="VFQ79_19355"
     CDS             3072..>3622
                     /locus_tag="VFQ79_19355"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HEU0141889.1"
                     /translation="MKRTKGPGRRRFLQTVALAGGIPAGSIAQQAGGSSGRTKAAAAP
                     IEYPRVFTGARLARIAFPLGGIGAGTISLGGRGQLRDWEIFNRPDKGKSPQYAFSSIW
                     VKSSKGAPVVSVLEAALTPPYEGSSGLGTANVPGLPRLESAVFTGEYPLARVDFRDRK
                     LPVRVSLEAFSPFFPLDPDESGLP"
BASE COUNT          684 a         1111 c         1083 g          744 t
ORIGIN      
        1 cgaaaccagg cgcttcagat gctcacttca gttggagtgc acgatcaggt agctctctat
       61 gtcttggaca atgggcttcg catagttcac gaatttaccg cggacacggt cgctctggcc
      121 cggaagattg cggccgaaca ggagcaggtg gtggacgctc ccgatccgga ggtgaaccct
      181 ctgacggatg cgagcgttgg ctcgctgcct ttttcagata tcgggcctgg tggtctctcc
      241 acgaccaagc gggaggagcg cttctttatc gaacgtcgcg ttacaggtac gttgaaggcg
      301 ttcgagatga tagcagcaca cctcgccgcc atacccggca ggaaaagcct gatctgggtt
      361 tccgccgggt ttcctccatc cctcggcttc gatctccctc cggtcggcaa acgccctgaa
      421 ggcttcgcgg acgctcgaac attcactccg gagttgaacc gtgcgctctc ccgcctaaac
      481 gaagccgatg tttccgtcta tccggtcgac gccagagggc tgagtcctaa ccctggcgtc
      541 tggaaaaaca tcgctaccat gcaagaactt gccgaacgta ccggcggaaa ggcatactac
      601 aaccgcaacg atctcgccca ggcgatgcgg agcgccctgg atgacgtccg cgtgagttac
      661 acgctcgcat actatctgcc gatggccgcc acccgcaggg acactctcca ccgcatccgg
      721 attcggatcc acaggccggg agtaaccgtc cgccaccgga gggagtatct ggagcgtgcg
      781 ggtggtgcgg ccattgctcc ggctgaggct aagtccgagg cgcggcaggc ctggagcagc
      841 cccttgaatt ccacggcgct gcgcatcgat gcgaagcccg tgcgaagcgg aaatacgttg
      901 gtcattcaaa ttgggttgga cgccagcggg atttcgttga ctcgaaaaga tggccggctg
      961 actggcgaac tattgatact tacggggttc aggaaagatg acggaacgcc gcttgagata
     1021 gccgattcga agtccgtcgc actcgcgctg cgcgagcaga cttaccaaaa ggtgctgcag
     1081 gagggtttga aagcgagggt gcagcttccc gtaccgggga atgcagcttc tgttcggctg
     1141 cttgtccggg atacggccac gaacaagttg gggactctca cgattcccct gcaggcgatt
     1201 cccgaaacgt agtggaggat aaacggtcgt tggcgcggcg cggtttcgtc gtcatcgttc
     1261 ccgacgcgtg gaaccctgag ccgttcctat caccgatagg aaatcgcgat cgaattgcag
     1321 cgcaccaggc ccggcgcatc gagggcatag gttgcggaac cgcctttgaa atcggcgggc
     1381 ttcagcactt gctcgtgtgc cttttcgccg cccgcggttt gatagcgata ggtcaccacc
     1441 accggtgcgc gcagtgcggc cgcgtcttgc agcttgaaat gaatcgtgtt tctgcccgcg
     1501 aagatctgcg gcagtgacat aatgccgttc tcgaaataga gatcgatggc aagagcgttc
     1561 agccccggca tactttggcg tcccggacgg ggcttcaccg aaaggcgaac ctggaaccgg
     1621 tacacgccgt gtatgctcac atcgcgcccg ttgaaccggg gccgtcctaa ctcgatgctc
     1681 atcttgccgg gacgcgacgg caacgtctgc cattcggacc acacgtccgg atcctgtgcg
     1741 ttgttggttt tcgcgcgcag cgtgcgaagg gctatgctgg cgtcgccttc ctccacaccc
     1801 gccaactctg cgttcaacgt gccgtccacc agaacgtatg ggctgtagaa atccaggacg
     1861 gcttcgcctc ccgccgccgc ctcacgcggc ctcagatacg gcgcggcggc cgaatgcatc
     1921 aggctggaac cgggtgccag cacatccttc aggtcttcct gcgccagagc cggagcatac
     1981 gtcatgcgtc cccatgcctg cccgatgctc ctgccgcccg ccatctccgg gtcatagttc
     2041 tcgttcttgg gaatcgtggc aagatacggc ttcgcccgtt catagtttgg atcgtatttc
     2101 ggccagttcc catccaacat ggtctccgtg acgcggtaga aacggccgga cggccggaac
     2161 ggcagctcgc gattcgcgct ctcgctgacg ggcctataga acttacgagc ggaattgtcc
     2221 cagaaacgct cgatggtcat tcctttgggc agcctgaaat ccatgtcgtg gaaacgcgtg
     2281 cccattttgt agtgggaatc gccgaatacg gatcgcttgg gcgaccccgc tttctcccac
     2341 gcgcgctggt tttcaaagta ggcgggctgt aaagcgagcg cccggtccca ctcctgcgcg
     2401 aagtattcaa agaacggcct gctgcggtgt ttaaagctcc gattgcgcag aatgttctcg
     2461 tcgttgccca cttccgccca atccagaatg cgggcgtccg gcgcatcgcg atcgatgagg
     2521 tagtatccgt agcgtggatc gaaggcgcca tggttgccgt ccaggcgcag gcggtacatg
     2581 gtgtgaaacc cgccgctctc gttttgtact accacgcgct cagccgcttt cgcgtcgcct
     2641 ttgtactcct gccacgccgc atccgccgcg cggctgcacg tatcgcaata gccccagccg
     2701 tatacgaaaa gactcttgtg cgcgtccagg acgtatcgct ccttgccaaa atcgccttcg
     2761 tgcgcctgaa tcataccgcc taccgccatg cggcagtata gtcgcagcca ttcgaagaac
     2821 gccttgccct gtgcggtctc cgatgccttt tcgacaccgg ccaggcgcat cacgtctttc
     2881 gtccacgtga caagatcggt cgcctgcggc cagcgttcgc ttaccaccag cggtccggat
     2941 acagtcggat gttccgtcgc ggccagacaa cccgccacca gcagcgctgc gataagaagg
     3001 cttttgttca tcagctctat tctcgctgtt tgtcccgcat tgtgatagaa cctacccgag
     3061 aggcctcata aatgaaacgc acgaaaggac ccggacggcg gcgctttctt cagacggtag
     3121 cgctggctgg tggaattcct gccgggagca ttgcccagca agccggcggg agtagtggaa
     3181 gaaccaaagc agcggcggcg cccatcgagt acccgcgcgt gtttacgggg gcccggcttg
     3241 cgcgcatcgc gtttccgttg ggcggcatcg gagccggaac tatcagcctc ggtggccgtg
     3301 gacagcttcg ggattgggag atcttcaacc ggcccgacaa gggcaagtcg cctcagtacg
     3361 cgttctcttc catttgggtg aagagctcca aaggagcgcc tgttgtatcg gtgctcgaag
     3421 cggcgctcac gccgccgtac gaagggtcga gcggcctggg cacggcgaat gtaccgggct
     3481 tacctcgatt ggaatcggcg gtgttcaccg gagagtatcc gttggcgcga gtggactttc
     3541 gcgaccggaa attgccggtt cgggtatcgc tcgaagcatt cagcccgttc tttcctctcg
     3601 acccggacga atcgggattg cc
//
LOCUS       DASTMK010000459         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_247618, whole genome shotgun sequence.
ACCESSION   DASTMK010000459 DASTMK010000000
VERSION     DASTMK010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247618"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFQ79_19360"
     CDS             complement(<1..446)
                     /locus_tag="VFQ79_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141890.1"
                     /translation="MRRLTTLLFFGLAAYSMAAGSEATILDRKHYSKVFGEERNYRIF
                     LPPGYDRTPDKRYPVIYFFHGWSERFNKPPRSGKGYDSGGDYGGDNIAAFVSRNDVIV
                     VRWDGYNPRTPGEDYPRPYNISPVETHRQFPLYFPELVRYIDSNYRT"
     gene            complement(446..>3784)
                     /locus_tag="VFQ79_19365"
     CDS             complement(446..>3784)
                     /locus_tag="VFQ79_19365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141891.1"
                     /translation="SISGQSNAEGNFEIPYLLPGEYRITAEQTGFKSFRRDGVEVRIG
                     ERLSIPIRMELGQISEQITVTAETPLLESAGANLGQVIDQRRIAELPIAHGNPFLLMT
                     LSPGVSYTQNPGLDRPFEPTHIVGYAMGGVRANRSEITLDGAPNTALNHRWGAGDLMA
                     GYTPPSDVVHEFKVQTATFDASIGHTQGGVTSVTLKSGGNGLHGTAYYSLLNPVLNAN
                     LFFANSAGQPKGHFIYNRWGTSATGPVYIPKLYNGKDRTFFTYGYEGIHESRAMGAAY
                     GSGTLTVPTARQREGDFSELLAVGSRYQIYDPATRVALGNGRYESQPFAGNVIPSSRI
                     NPVARNILNYYSLPNVPGTADGTNNLIRVNDPEVIKYFNHIGRVDHNISANNRMFARF
                     NTYKRFSNSSDWFRSAPTGGYSDWKQHSFSIDDVHNLSATTFMNVRYSFFRLSIYQYP
                     QPESVGFDLASLGFPRSYTDAIDPGVRAFPAISIAGYTGTQNNWWRYPTHNHSLEGNV
                     TSIKGNHTLRFGVDGRQYRNFQYEPHNASSGIFEFGNTWTLGPLDNSPASPIGQGLAG
                     MLLGLPTGGGVDRKASFAAQSTVVSAYVQDDWRITPKLIVNLGLRYELESAMTERFNR
                     TVRGYDFSTPSPLEAQVRANYAANPIAELPANEFRLIGGLTFPGVGSQPRALWNRDTN
                     NIMPRAGFAYTFDQKTVVRGGFGIYFGPLGAQRGDVIQLGFSQRTEFVPTLDNGLTFI
                     ADLSNPFPNGIQEAPGAADGLLTYVGRSARFFEESPRAPRQQRWQLGVQRELPQRVLM
                     EVAYLGNYGDSLELTRDLRALPTEYLSRSPFRDNPHINYMTQQVRNPFYPLLPGTGLS
                     GQNVSRSYLLSGPEYSQFTGLTSTSYEGYSWYHAMQVRTERRFAAGWSVNAAYTWSKS
                     MEAIGRLNGYLSPLEYAISTQDRPHRFVISGLWELPFGRGKRLLNAAPAVLDKFVGGW
                     QVQGIYTAQSGPPIGFGNVLYMGDIHDITLPPGERTVTRWFNTQGFERVPANQLSFNY
                     RTFSSRFSDIRADGVNSWDLSVLKNTRIHERVNAQFRGEFLNAFNHPMFNAPNTSPTS
                     TAFGQVTSQRGYPRRIQLMLKLIF"
BASE COUNT          750 a         1056 c         1214 g          764 t
ORIGIN      
        1 gttcgataat tcgaatcgat gtagcgaacc agttccggga agtagagcgg gaactggcga
       61 tgcgtctcga ccgggctgat gttatagggg cgcgggtagt cctcgccggg tgtgcgcgga
      121 ttgtagccgt cccaccggac tacgatgacg tcgttgcggc tgacgaaggc ggcaatgttg
      181 tcgccgccat agtcgccgcc tgaatcgtat cccttgccgc tgcgcggcgg cttgttaaaa
      241 cgctcgctcc agccgtggaa aaagtagata accggatagc gcttatcggg cgtccggtca
      301 tatccgggcg gaagaaaaat gcgatagttc cgttcttcgc cgaatacttt gctgtaatgc
      361 ttccggtcga gaatggtggc ttcggaaccg gccgccatgg aataggcggc cagtccgaag
      421 aaaagcaggg ttgtgagacg gcgcattaga aaatcaattt cagcataagc tgaatgcgcc
      481 gcgggtatcc gcgctggctc gtcacctgcc cgaaggcggt gctggtgggg ctggtgttcg
      541 gagcattgaa catcggatga ttgaacgcgt taagaaactc tccgcggaat tgcgcattga
      601 ctctctcgtg aatgcgcgtg ttcttcagaa cggaaagatc ccaactgttt acgccgtcgg
      661 cgcgtatatc gctaaaccgg ctcgagaacg tgcggtagtt gaacgagagc tgatttgccg
      721 gcacacgctc gaaaccctgg gtattgaacc agcgggtgac ggtgcgctct cccggcggca
      781 aagtgatgtc atggatatcg cccatataga gcacgttgcc gaagccgatg ggaggaccgc
      841 tctgggcggt gtagatgccc tgcacctgcc agccgccgac gaatttgtcg aggactgccg
      901 gcgcggcatt gagcaggcgc ttgccgcgcc cgaacggaag ttcccagagg ccgctgatca
      961 cgaaacggtg cggccggtcc tgggtggaga tggcgtactc caacggcgac aggtagccat
     1021 tcaaccggcc gatggcctcc atgctcttcg accacgtgta ggcggcgttc acactccatc
     1081 cggcagcgaa ccgtcgctcg gtgcgcactt gcatggcgtg ataccaggag tatccttcat
     1141 agctggtgct ggtcaatccg gtgaactgag aatactccgg tccagacaac agataagatc
     1201 gcgaaacgtt ctgtccggaa aggccggtgc cgggtaacaa cggataaaac ggattgcgca
     1261 cctgctgagt catgtagttg atatgcgggt tatcgcggaa gggcgagcgg ctcaggtact
     1321 ccgtgggcag cgcccgcaag tcgcgcgtga gttccaggct gtcgccgtaa ttgccgagat
     1381 aggcgacttc catcagcacc cgctgcggca attcccgttg cacgcccaac tgccagcgct
     1441 gctgacgcgg cgcgcgcggg gactcctcga agaagcgggc gctgcgcccc acgtaggtga
     1501 gcaagccgtc cgcagccccc ggagcctcct gaattccatt cgggaatggg ttgctgaggt
     1561 ccgcgatgaa ggtcaaccca ttgtccagcg tcggcacgaa ctccgttcgc tggctgaagc
     1621 cgagttggat gacatcgccg cgctgcgcgc ccagcgggcc gaagtaaata ccaaagccgc
     1681 cgcggacgac cgtcttctga tcgaaggtgt aagcgaagcc ggcgcgcggc atgatgttgt
     1741 tggtgtcccg gttccagagc gcgcgcggtt gggagccgac gcccgggaac gtcaacccgc
     1801 cgataagccg gaattcattg gcaggcagtt cggcgatcgg attggcggca tagttcgcgc
     1861 gtacctgcgc ttcgagcggg ctgggggtcg agaaatcgta accccgaacg gtacggttaa
     1921 agcgctcagt catcgcgctc tcaagctcgt agcgcagtcc caggttgacg atcagcttcg
     1981 gtgtgatgcg ccagtcgtcc tgtacgtacg cggacacgac ggtagattgg gcggcgaaac
     2041 tggctttgcg gtcgacgccg ccgccggtgg gcaggcccag aagcatgccg gcaagtccct
     2101 ggccgatggg cgaagctggc gagttgtcaa gagggccgag cgtccaggtg ttgccgaatt
     2161 cgaagatgcc actcgatgcg ttgtgcggct cgtactggaa attcctgtac tgccgcccgt
     2221 cgacgccgaa ccgcagggtg tggttgccct tgatggaagt aacgttcccc tcgaggctgt
     2281 gattgtgggt ggggtaccgc caccagttgt tctgcgttcc ggtgtagccg gcgatgctga
     2341 tggcagggaa tgcccgcacg cccggatcga tcgcgtccgt gtaggacctg ggaaagccca
     2401 gactggcgag gtcgaatccg acgctctccg gttgcggata ttgatagatc gagagccgga
     2461 agaaactgta gcggacattc atgaacgtcg tggcactcaa gttgtgcacg tcgtcgattg
     2521 agaacgaatg ctgcttccag tcggaataac cgccggtcgg cgcgctgcgg aaccagtcgc
     2581 tcgaattact gaaacgcttg taggtgttga agcgcgcgaa catccggttg ttcgcgctga
     2641 tgttgtggtc gacacggccg atgtgattga agtacttgat cacttcgggg tcgttcacgc
     2701 ggatcaggtt gttggttccg tccgccgtgc ccggcacgtt cggcagcgag tagtagttga
     2761 gaatattccg ggcgaccggg ttgatgcggc tggatggaat gacgttgccc gcaaaaggct
     2821 gcgactcgta acgtccgttc cccaacgcga cgcgcgtagc cggatcgtaa atctggtaac
     2881 gcgaccctac cgccaggagc tccgagaaat caccctcgcg ctgtcgcgcc gtgggcaccg
     2941 ttaaagttcc ggagccatag gcagcgccca tcgcgcggct ctcatgaatg ccttcatagc
     3001 cgtaagtgaa aaacgtccgg tctttgccgt tgtagagttt cggaatataa acgggacccg
     3061 tcgcgctggt gccccagcgg ttgtagataa agtgcccctt gggctgtccc gctgagttcg
     3121 caaaaaacag attggcgttg agaacggggt tcaatagcga atagtaagca gttccgtgca
     3181 aaccgttgcc cccggatttc aacgtgacgc tggtgacgcc gccctgggtg tggccgatac
     3241 tggcatcgaa agtggcggtc tgcactttga actcatgcac cacgtcggaa ggcggtgtgt
     3301 agcccgccat cagatcgcct gcgccccagc gatggttgag cgctgtgttc ggcgccccgt
     3361 ccagcgtgat ctcgctgcga ttcgcacgaa cgccgcccat cgcgtatccg acgatgtgcg
     3421 ttggctcaaa cgggcggtcc aacccgggat tctgggtata agatacgcca gggctgaggg
     3481 tcatcaggag aaacggattc ccgtgagcga taggcaactc ggcgatacgg cgctgatcga
     3541 tgacctgtcc taagttagcg ccggcggact ccagcagagg cgtttccgcc gttaccgtga
     3601 tttgctcgct gatctgccct aattccatgc gaatggggat ggaaagacgc tcgccgatac
     3661 gcacttcgac tccgtcgcgt cgaaacgact tgaagccggt ctgttcggcc gtaatccggt
     3721 attcaccggg caaaagatag gggatttcga aattcccttc cgcattggat tggccgctta
     3781 tcga
//
LOCUS       DASTMK010000460        12320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_566278, whole genome shotgun sequence.
ACCESSION   DASTMK010000460 DASTMK010000000
VERSION     DASTMK010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566278"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VFQ79_19370"
     CDS             complement(<1..1285)
                     /locus_tag="VFQ79_19370"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0141892.1"
                     /translation="MLPLLVLSPYLLIPAAFWLGGRSARVAGLLALWPAALALYFGLM
                     LRSVGVSGPIEASLPWAASIGVDLSFYADGLSLLFAVVVAGIGALIVLYGSRYFGGEP
                     RAGRFQATLFAFMGSMLGLVLADNLFLLFVFWELTGFTSFLLIGFEHERPEARASAVQ
                     ALIVTGLGGLCLLAAAVLIEQSTGAAALSSLLGRGLNLANEEFYPAIVVLLLLAAFTK
                     SAQTPFHFWLPNAMTAPTPVSAYLHSATMVKAGIYLVARATPFAGATPLWTGLVTGAG
                     AITMIVGAWRAVAETDLKRVLAYSTVSALGLMMMLLGVGTEEAVLAALVYLLAHACYK
                     GALFLVAGTIEHETGTRDITGLGGLRHAMPAAALAGALAACSMAGLPFFLGFLGKELL
                     YESLLAWSTTLLIGAIAASALLGTAGLIAGVSPFAG"
     gene            1429..1791
                     /locus_tag="VFQ79_19375"
     CDS             1429..1791
                     /locus_tag="VFQ79_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141893.1"
                     /translation="MAVAIFAIGYLPQYSRYRQARQEYLDTAFEHRLCAIRDAASLTY
                     LEATRKNFGIAAQHSTQYFNRVRDASQDASDPGLKSSLQELLTARDMITSGLSSADPA
                     VIGELQALVQRTFEITRP"
     gene            1795..2664
                     /locus_tag="VFQ79_19380"
     CDS             1795..2664
                     /locus_tag="VFQ79_19380"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0141894.1"
                     /translation="MGFSMRLLRRSEWLLIVYFLYTSLLALLLPLRSPVPVITCTLNL
                     TVIGGFFLLAYADSLRRRPFLSIVRDWYPSPLMLLAYREMGWFAPTRHDYRLEQSWII
                     WDRILLNDLGLRTAIESLGPVIPSILELSYSLVYTMATFSVAMLYAYHKRERVDRFLT
                     IFLFGILAAYALFPYFPSEPPRAVFPNEDLPGVLTPFRRFNLALLGGYGIHTSVFPSA
                     HVSGAFSAAFAMFGILPEKRWVASVLLVLAVLITTATIYGRYHYAVDALAGFAISIAA
                     VFAGRALFPRAAR"
     gene            complement(2667..4544)
                     /locus_tag="VFQ79_19385"
     CDS             complement(2667..4544)
                     /locus_tag="VFQ79_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141895.1"
                     /translation="MKRAFVACCVLALTALLSPVKAAEAIVIKSPMPPPDWAVLQREL
                     LRYSSLACERFAEKYVDERGYLLHTIRWGTLDGPDDAIETFYNWTLLHALGGSDAVLE
                     IYKRAQEGHWNQYNELRTKLTEIASNGAYYKEFITQSDWFHTGEGMRAFMFLGLSEPT
                     NEMYIQRMKRFAGMYMNEDPEAPNYDPVNKVIKSIWNGSKGPMMRKATVYDWVGDPVP
                     GGFHLLHNPAGRGKLLDMAKWYPRMLAHCNEYLDSVGDNSLNLAATNLALNAYMLTGE
                     KKYYDWLVEYVNAWKERTAATGGNIPSNIGLDGKPGGEYNGQWWKGTYGWNFTIFDGE
                     LEQIAHRNYFTAGSWPGFSNAMLITGDHGYVDVLRKQMDNIYAQKKVENGTVLLPQMY
                     GDPRGYKHNGKPEWYHYTKNLFGDRLAEIYLWSMDRKDLERVPKTGWIAYLEGEDPEY
                     PVKALRDDFARVRRAVQEMRDDPTTPDTRLADYLLSANPAVTNALTNLTLGGYFANGR
                     IWTLHSRFRYFDPAHRRSGLPEDVSALVEKLGPDSATVVLVNTNPVGARTVAVQAGGY
                     GEHRFDAVTVDGKSEPIGGPLLTVRLEPGSGARLQFKMSRYAYRPTLAQPWDRGVATA
                     N"
     gene            4543..8745
                     /locus_tag="VFQ79_19390"
     CDS             4543..8745
                     /locus_tag="VFQ79_19390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0141896.1"
                     /translation="MISSIYRDPAGLRNYPFRVLMEKGAAFRLINVRMPLSRFHPLVA
                     EWFTSALGSPTEPQLLGWPEIQAGRDVLISAPTGSGKTLAAFLSCLNDLVSASTTGSL
                     ADETHVLYVSPLKALSNDIHRNLDLPLEGISALAAARGVNLAPVRTALRTGDTPAWER
                     QRMTREAPHILVTTPESLFILLTAEKPRQILRNVRTLIVDEIHAMADDKRGSHLALSL
                     VRLDALTRAAYGGRAQRIGLSATVKPLSEVAAFLGPDTTVVNVGHRREMDLAVDVPGD
                     ELGPVASNELWSEIYDRIAGLIHEHRTTLVFVNTRRLSERVAHALSQRVGESLVLPHH
                     GSLSRPLRFEAEARLKSGDLKAVVATASLELGIDIGTVDLACQIGSPRSIAVALQRIG
                     RSGHWLGAKPKGRLFPTTRDELLECAALVSAIHAGELERIEIPRNALDILAQQIVAAS
                     AAEPWQEDDLFRLVREAYPYRDLPRQDFDEVVEMLSEGTATARGRSGAFLHRDRVNGR
                     VRGRRGARLAAITSGGAIPETGNYTVVAEPEGKTIGSVEEDFAVESMAGDIFLLGTHS
                     WRIRRVEPGRVRVEDAKGAAPSIPFWFGEAPGRSPELSRAVSDIRQRISADPEAARAF
                     LLSNGGLDPAGAVQAIAYARAGEASLGAMPSHNTIVAERFFDEAGGMQLILHAPFGSR
                     INRAWGLALRKRFCRSFNFELQAAATDNGIVISLSDQHAFPLEVVFEFVKAATAEDVL
                     TQALLAAPMFTARWRWNATRALTILRFSGGKKVPAPIQRMRSDDLLASVFPDQVACAE
                     NLTGPIRIPDHPLVKETIDNCLHEAMDLDGLLRILEAMDSGAIRTVAVDNAQPSPLSH
                     EILNANPYAFLDDAPLEERRARAVQLRSSLRSDFAGGAGVLDASAIEQVSKEAWPTVR
                     DADELHDALLSLIAVPPAPEWQVFFEALSAEGRATDRRRGGKDFWVAAERLTSFDDPL
                     ATVRGWMESTGPVTIGELSDRLAFPPVEIEQALATLEGEGQVLCGQFRPPEQSQGVEW
                     CNRRILARIHRYTIGRLRREIEPVSALDFHRFLLRWQHVAAGSQLHGADGTLHIVRQL
                     QGYEIPAAAWEPQVLARRIARYGHEDLDRLCLSGEVMWGRLSPHSAFEDSEGRRVRPT
                     RIAPITFFLRADADWLIADAGPSEAAALSHAAQEVLNELKTHGASFFGDIVRATRRLA
                     SEVEDALWELVAAGLVTADGFENLRALIDPKRRRGEGRGRSARPRHAAGRWALLRRPA
                     ALPEEERAAKFADQLLLRWGVLFRDLLARESLAPKWRELLPVLRKKEAQGELRGGRFV
                     SGFTGEQFARPEAVELLRAVRRAGAHPEDTLTIPNADPLNLAGIILPGPRISALTSGS
                     LRLADYILPEPVLEPEAAGAAR"
     gene            9042..9392
                     /locus_tag="VFQ79_19395"
     CDS             9042..9392
                     /locus_tag="VFQ79_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141897.1"
                     /translation="MYWRGARLRWLGRQCKVALNGTWVAVLSPNTYSVIDAEEGNLKF
                     CGASNVLSPTWRSLLFLHATGGETYFVECSPGGDAWGASDPTLSETEQEESWKAVSRL
                     RVVTFRLKSPRRTE"
     gene            9389..9868
                     /locus_tag="VFQ79_19400"
     CDS             9389..9868
                     /locus_tag="VFQ79_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141898.1"
                     /translation="MSTGVFLFVVAEVIAPLSAGHPEETPSGEYTWKNVVYLGGVPGV
                     RVDRGDFDQTLTVTPLKLIVIVQPPPDLDASKLPRKRPPRVLFEIPRDSIIGVNYEGF
                     RHDMPIAQIWFGIPRYWPKATDHLLVLTYRLCDGRETEVLLRVDKNHFREILQALRN"
     gene            10046..10318
                     /locus_tag="VFQ79_19405"
     CDS             10046..10318
                     /locus_tag="VFQ79_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HEU0141899.1"
                     /translation="MPRGNPSPKLAITVDPDIHESILRAAAREGVSVSAWMTAAAREA
                     LRQRAGLAAVALWEKQYGRLTTEEMNEARRSVRAQLRASRTVRRPA"
     gene            10315..10695
                     /locus_tag="VFQ79_19410"
     CDS             10315..10695
                     /locus_tag="VFQ79_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141900.1"
                     /translation="MSAVVYDVAVLVAADKNERRAWAEHKVRLELGVVPLVPAPVVAQ
                     VSRSPQQAQLRRFLTGCVVVPLRESEAHEAGRLLGMTKTADVVDAVVVTTALRQKATI
                     LTSDPGDIERLVRASGREVAVVAI"
     gene            complement(10692..11303)
                     /locus_tag="VFQ79_19415"
     CDS             complement(10692..11303)
                     /locus_tag="VFQ79_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141901.1"
                     /translation="MKLFIWAVSVLLVTPAFTQTNRWFDQTLGQVKEYLQLTDGQVQT
                     ILANNDEYNQWSIGKQNRIWQVQSEIVEETAKDPLSVDALGIRYAEIEMICRQMKEQA
                     NQYQTRNVDILDQNQKARLKTLEDALRLVSVISQAQSSNLAGEITTAPAFFTSTTTGT
                     SGTVLGGIIGNATGCILPFPAAFSGSRVPAPASTTGQVVFALP"
     gene            complement(11356..12153)
                     /locus_tag="VFQ79_19420"
     CDS             complement(11356..12153)
                     /locus_tag="VFQ79_19420"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU0141902.1"
                     /translation="MPDSRTLPILNHPLDEPSAFTADALMHSVRSMRMIPGGTVPPLC
                     ILEFDGDLTDWLVHTGRAKPVPSWPCFHTTMYAVESEGMTCGIVARTIGGPYAVLVAE
                     QLAATGAGWIIGLTSAGRVSPELPLPCLVVVTAAVRDEGTSLHYLPPGAEVSCPDPIP
                     ALLEKELPATGWAVRSGKVWTTDAPYRETRAQLQHWADEGVVAVEMQAASLFAFGKAR
                     GVPVGCVAMVSNAVDHEGDQFDTGSQEDGLRIVAACARAFRNRPDVT"
BASE COUNT         2292 a         3736 c         3763 g         2529 t
ORIGIN      
        1 ctcccgcaaa aggagagacg ccggcgatca gaccggccgt gccgagcagt gcactcgcgg
       61 cgattgcccc aatcagcagc gtggtcgacc atgccagcaa ggattcatag agcagttcct
      121 tgccgaggaa gcccagaaag aagggcagtc ccgccatgga gcaggcggca agcgccccgg
      181 caagcgccgc cgccggcatg gcatgcctga gcccgccgag tccggttatg tccctggtgc
      241 cggtctcatg ttcaatggtg cccgcgacca gaaaaagcgc acctttgtag caggcatgag
      301 cgagcaggta caccagggcc gccaataccg cctcttcggt gccgactccg agcaacatca
      361 tcatcaaacc gagcgcgctc acggtggaat acgccagcac gcgtttcaaa tctgtttcgg
      421 caaccgcacg ccatgcgccc acgatcattg tgatggcacc cgcgccggtt acgagccccg
      481 tccacagggg agtggcgccg gcgaacggcg ttgctctggc taccaggtag atgcccgcct
      541 tcaccatcgt ggccgagtgc aggtaagcgc tcaccggggt cggagccgtc atcgcgttcg
      601 gaagccagaa atgaaacggc gtctgggcgg acttggtgaa cgccgccaac agcagcagca
      661 ccacgatggc cgggtagaac tcttcgttcg caagatttag ccctcgcccc agcagcgacg
      721 agagagccgc cgcgccggtc gattgctcga tcaacacggc ggcagccagc aggcacaggc
      781 ctcccaatcc ggttacaatc agcgcctgca ccgcggaggc acgcgcttcc ggccgttcat
      841 gttcgaagcc aatcaggagg aaggaggtga atcccgtcag ttcccagaaa acgaacagca
      901 aaaacagatt gtcggcgagc accaggccca gcatcgagcc catgaaggcg aataacgttg
      961 cctgaaagcg tccggccctt ggttcgccgc cgaaataccg gcttccgtag agcactatga
     1021 gtgcgccgat accggcgacg accactgcaa acagcaagct caagccatcg gcataaaagg
     1081 agagatcaac ccctatggat gcggcccagg gaagagatgc ttcgatgggt ccggagaccc
     1141 ccacggaccg cagcatcagg ccgaagtata aagcgagcgc cgcgggccac aatgcgagca
     1201 ggcccgccac ccgcgcggat ctcccaccga gccaaaaggc cgccggaatc aacagatacg
     1261 gcgatagaac gaggagaggg agcatcaaat gtcttcaaca tggcatagcc cgctccggag
     1321 tgcataggag cgcgcagacc gtgcgcctga cgacgctatt ttgctattgt agccttcggc
     1381 agcactcggg tacagcagat gggacgaaaa atattaatcg gaattctggt ggccgtagct
     1441 atttttgcga tcggttacct gccgcagtat tcccggtatc gccaggcacg gcaggagtat
     1501 ttggatacgg cgttcgagca caggctctgc gcaatacgcg atgccgccag cctcacctat
     1561 cttgaagcca ccaggaaaaa cttcggtatc gccgcgcagc actcgacaca atatttcaat
     1621 cgtgtgcgcg acgcctcgca ggacgcgagc gacccggggt tgaaaagttc gttgcaggaa
     1681 ttgttgactg cgcgtgacat gattacgtcc ggtctcagta gcgccgaccc tgccgtcatt
     1741 ggagaattgc aggcgctggt gcaaagaact tttgagatca cccggccctg aaaaatgggt
     1801 ttctcgatgc ggttgttgcg ccgctcggaa tggctgctca tcgtgtactt cctgtacacg
     1861 tcgctgcttg cgttgctgct gcctctccgg agccctgtcc cggttatcac ctgcacgcta
     1921 aacctgactg taatcggggg cttctttctc ctggcctatg ccgattcgct gcgccgccgc
     1981 ccattcttaa gcattgtccg cgactggtat ccctcacccc ttatgctgct ggcctatcgg
     2041 gaaatgggat ggttcgcccc aacccggcac gactaccgcc tggaacagag ctggattatc
     2101 tgggaccgca tcctgctaaa tgatcttggt ttgcgcacgg cgatcgaatc gttgggcccc
     2161 gtcatcccgt cgatcctgga gctgtcgtac tctctcgttt acaccatggc gacgttctcg
     2221 gtcgcgatgc tatatgccta ccataagcgc gaacgggtgg accgctttct caccatcttc
     2281 ctgttcggca ttctcgctgc ctacgctcta ttcccgtatt tcccgtcgga gccgccgcga
     2341 gcggtgttcc cgaacgaaga tctgccaggc gtgctgacac cctttcgccg gttcaatctg
     2401 gcgttgctcg gaggttacgg cattcacacg agcgtctttc ccagcgccca cgtttcaggc
     2461 gcattttccg cggccttcgc catgttcgga atactgcccg agaagcgctg ggtagcgtcc
     2521 gttctgctgg tgctggccgt tttgattacc acggccacca tctacgggcg ctatcactac
     2581 gccgtggatg cgctggcggg attcgccatt agcattgcgg cggtgtttgc cggccgcgct
     2641 ctcttcccgc gagccgccag ataatttcag ttggccgttg caactccgcg gtcccagggt
     2701 tgggcgagtg taggacggta tgcgtagcgg ctcattttga actggagacg tgcgccgctg
     2761 cccggctcca gccggaccgt aagcagcggt ccgccaatgg gttcactctt cccgtccacc
     2821 gtcaccgcat cgaacctgtg ttccccgtaa ccgcccgcct gcaccgccac tgtgcgtgcg
     2881 cccacagggt ttgtatttac cagcaccacg gtggcggaat ccggtcccag cttttccacc
     2941 agcgcgctaa catcttcggg cagtccggac cggcggtgtg ccgggtcgaa atagcggaag
     3001 cggctgtgca gggtccagat tctcccgttg gcgaaatatc cgccgagtgt caggttcgtc
     3061 agcgcattgg tgaccgcggg gttggcgctc agcaggtaat cggccaggcg cgtatcgggc
     3121 gtggtggggt catcacgcat ctcctgcacg gcgcgccgca ccctcgcgaa atcgtcccgc
     3181 agcgctttga cgggatactc aggatcctcg ccttcgagat aggcgatcca gccggttttc
     3241 ggcacgcgct ccaggtcctt gcggtccatc gaccagagat aaatctccgc cagccggtcg
     3301 ccgaacaaat tcttcgtata gtggtaccac tccggcttgc cgttgtgctt gtacccgcgc
     3361 gggtcgccat acatctgcgg cagcagcaca gtgccgttct cgaccttctt ctgcgcgtag
     3421 atgttgtcca tctgcttccg gagaacgtct acataaccgt gatcgccggt aatgagcatg
     3481 gcgttgctga agccgggcca gcttccggcg gtgaagtagt tgcgatgcgc gatctgctcc
     3541 aactccccat cgaagatcgt gaagttccag ccatacgtgc ccttccacca ctgcccgttg
     3601 tattctccgc cgggtttgcc gtcgaggccg atgttgcttg ggatgttgcc gcccgtagcc
     3661 gccgttcgtt ccttccaggc attgacgtat tccaccagcc agtcgtagta ttttttctcc
     3721 ccggtcagca tgtaggcgtt cagggccagg ttggttgcgg caagattcag agaattgtcg
     3781 ccgacgctgt ccagatactc gttgcaatgc gcgagcatgc gcggatacca cttcgccatg
     3841 tcgagaagct tgccgcgtcc ggcagggttg tggagcaggt gaaagcctcc gggcacggga
     3901 tcgccgaccc agtcgtagac cgtcgccttt cgcatcatgg ggcccttgct gccgttccag
     3961 atgctcttga ttaccttgtt gacgggatcg tagttgggcg cttccgggtc ttcgttcata
     4021 tacatcccgg caaagcgttt cattcgctgg atgtacatct cgttcgtggg ttccgacaag
     4081 ccgaggaaca tgaaggcgcg cattccttcg cccgtgtgga accaatccga ttgcgtgatg
     4141 aattctttgt agtaggcccc gttgctggcg atctccgtca gcttcgtccg cagctcgttg
     4201 tactggttcc agtgtccttc ctgcgcgcgt ttgtaaatct ccagaacggc atccgagccg
     4261 cccagcgcgt gcagcagcgt ccaattgtaa aaggtctcaa tggcatcatc cggaccatcg
     4321 agcgtacccc agcggatggt atggagcaga tagccgcgtt cgtcgacata tttttcggcg
     4381 aaccgctcgc acgcaagcga gctgtagcgg agcaactcgc gctgcaacac cgcccagtcc
     4441 ggcggcggca tcggcgattt gatgacaatg gcttcggcgg ctttcactgg agacaacaat
     4501 gccgtcaacg ccagtacaca acaggccacg aatgcgcgtt tcatgatctc aagtatttat
     4561 cgtgatccgg cgggtctgcg caactatcct tttcgcgttc taatggaaaa aggggctgcc
     4621 tttcggctga tcaacgtccg tatgccgctt tccaggtttc atcctttagt ggcggagtgg
     4681 ttcacctccg ctctgggatc tccaacagaa cctcagcttt tgggctggcc ggagatacag
     4741 gccggccgtg atgtcctgat ctccgctcct actggttcgg gtaagacgct ggccgcgttt
     4801 ctgagctgcc tgaacgatct cgtgagtgcg tccaccaccg ggtcgctggc ggatgaaacg
     4861 cacgtcctgt acgtttcgcc tctcaaagcc ctgagcaacg atattcaccg gaacctcgat
     4921 ctgccgctcg aaggtatttc ggctcttgcg gcggcgcgcg gcgtgaacct ggcgccggtc
     4981 cgcacggctc tccgcaccgg agacacgccc gcatgggagc gccagcgcat gacgcgggaa
     5041 gcaccacaca tcctggtgac caccccggag tctttgttca ttctgctgac ggcggagaaa
     5101 ccacgccaga ttttgcggaa cgtccggact ctgatcgtag acgaaataca cgctatggcc
     5161 gatgataagc gcggctcgca tctcgcgctt tcgctcgtgc gtctggatgc gttgacgcgt
     5221 gcagcctatg gcggccgggc gcaaagaatc ggtctttccg caacggtaaa gccattgagc
     5281 gaagtggccg cctttctcgg ccccgatacc acggtggtga acgtcggcca ccggcgcgag
     5341 atggaccttg cggtcgatgt tcccggcgac gaattgggcc cggtcgcgag caatgagctg
     5401 tggagtgaaa tctatgaccg catcgccggg ctgattcacg agcatcgcac tacgctcgta
     5461 ttcgtcaaca cgcggcggct ttccgaacgc gtggctcacg cgctttcgca gcgtgtgggc
     5521 gaaagcctgg ttctgccgca ccacggcagc ctgtcgcgtc cgttgcgctt tgaagcggag
     5581 gcccgcctga aatcgggcga tctgaaggcg gtggtggcca ccgcttccct cgaactgggc
     5641 atcgacatcg gcacggtaga tctggcctgc cagatcgggt cgccacggtc cattgccgtt
     5701 gcgctccaga gaatcggccg ctccggacac tggctggggg cgaagccgaa gggccgcctg
     5761 tttcccacca cgcgcgacga actgctggaa tgcgccgcgc tggtgtccgc cattcacgcc
     5821 ggggaactgg agcggatcga aattccccgg aacgcactgg acattctcgc tcaacagatc
     5881 gtggccgcct ctgccgccga accgtggcag gaagacgatc tgttccggct cgtccgggaa
     5941 gcctatccgt atcgcgatct gccgcgtcag gattttgacg aggtcgtcga gatgctttcc
     6001 gaaggcaccg cgactgcgcg cggccgtagc ggagcgtttc ttcatcgcga ccgcgtgaac
     6061 ggccgggtgc ggggccgccg cggcgcgcgc ctggcggcga tcacctccgg cggcgcaatt
     6121 cccgaaaccg gcaactacac ggtggtggcc gaaccggagg gcaaaaccat cggctccgtg
     6181 gaagaggatt tcgccgtgga aagcatggcc ggcgacattt ttcttctggg cacccattcc
     6241 tggcgtatcc ggcgggtgga accgggtcgt gtgcgggtgg aggatgcaaa aggcgccgct
     6301 ccatcgatcc cgttctggtt cggagaggcc cccggccgct caccggagct ttcccgggcc
     6361 gtctctgaca ttcgacaacg catttcggcc gatcccgagg cggcgcgcgc tttcctactg
     6421 agcaatggtg ggctcgatcc ggcgggggcg gtgcaggcca tcgcctatgc acgggcgggg
     6481 gaagcttcgc tcggcgcgat gccttcccac aacacgattg tcgcggagcg tttcttcgat
     6541 gaggcgggag gaatgcagtt gattttgcac gcgcccttcg gatcgcgcat caaccgcgct
     6601 tggggccttg ctctgcgcaa gcggttttgc cgcagcttca atttcgagct tcaggctgcc
     6661 gccaccgaca acggtatcgt aatttcgcta agcgaccagc atgcgttccc gctggaggtt
     6721 gtattcgaat tcgtgaaggc cgcaacagcg gaggacgtgt tgacacaggc gttgctggcc
     6781 gcgcccatgt tcaccgcccg ctggcgttgg aacgccacac gggcgctgac gattctgcgt
     6841 ttcagcggcg gcaaaaaggt gcccgcgccg attcaacgca tgcgctcgga cgatctgctg
     6901 gcgtccgtgt ttcccgacca ggtagcctgc gccgagaacc tgaccggccc cattcgcatc
     6961 ccggatcatc cgctggtaaa agagaccatc gacaactgtc tgcacgaggc gatggatctc
     7021 gacgggctgc tgcgcattct ggaagcgatg gacagcggtg cgattcgtac agtggcggtg
     7081 gacaacgcgc agccttcgcc gttgtcgcat gagatcctga atgccaaccc gtacgcgttt
     7141 ctggatgatg cgccgctcga agagcgccgc gcccgcgccg tgcagttgcg cagctcgctc
     7201 cgaagcgatt tcgcgggtgg agctggagtg ctcgacgctt ccgccatcga gcaggtctcg
     7261 aaggaagcct ggcccacggt tcgtgacgcg gatgagttgc acgatgctct gctgagcctg
     7321 atcgcggtgc cgcctgcgcc cgaatggcag gtgttcttcg aagccctgtc ggccgaagga
     7381 cgggcaacgg atcggcggcg cggcggcaag gatttctggg tcgccgctga gcgtttgacg
     7441 tccttcgatg accctctcgc gaccgtgcgt ggatggatgg aatcgacggg accggtcacg
     7501 atcggcgagc ttagcgacag actcgcgttt ccgccggtag agattgagca ggcgcttgcg
     7561 actctggaag gcgaaggcca ggtgctttgc gggcagttcc gtcctccgga acaatcccaa
     7621 ggcgtcgagt ggtgcaaccg ccgcattctg gcgcgcattc atcgctacac gatcggacgt
     7681 ttgcggcgtg aaatcgaacc ggtctcggct ctcgacttcc atcggtttct gctccgatgg
     7741 cagcacgtcg cagcgggctc gcagttgcat ggagccgacg gaacgctcca catcgtgcgc
     7801 cagttgcagg gttatgaaat ccccgcggcg gcgtgggagc cacaggtgct ggcgcgccgg
     7861 atcgcccgat acggccacga agatctggac cgtctctgcc tttccggtga ggtcatgtgg
     7921 ggacggctct cgccgcacag cgcattcgag gattccgaag gccgccgcgt gcgccccaca
     7981 cgcattgcgc cgattacgtt ttttctgcgg gcggatgccg attggctgat cgccgatgcc
     8041 ggaccttccg aggccgccgc gctgtctcat gccgcgcagg aggtgctcaa cgagttgaag
     8101 acgcacgggg cttcgttctt cggcgatatc gtgcgggcca ccaggcggct tgccagtgag
     8161 gtggaagacg cgttgtggga attggtcgcc gccggtctgg tgaccgccga tgggtttgag
     8221 aatcttcggg cgttgatcga tccgaagcgg agacgcgggg aagggcgggg cagaagcgct
     8281 cgtccacgtc atgccgccgg acgctgggct ctgcttcgcc gtcccgccgc tttaccagag
     8341 gaggagcgcg ccgcgaaatt tgccgatcag ttgcttctgc gctggggtgt gctgtttcgc
     8401 gatctgctgg cgcgtgagtc cctcgctccc aaatggcgcg agttgctacc ggtgctccgg
     8461 aagaaggagg cgcaaggaga attgcgcgga gggcgttttg tttccggatt cactggcgag
     8521 cagtttgccc gcccggaagc cgtcgaactg ctgcgcgccg tgcgccgtgc cggagcccat
     8581 cccgaagata ccctcaccat tccgaatgcc gatccgttga acctggccgg tatcatcctg
     8641 cccggtcccc gcatcagcgc attaacttca ggaagcctgc gcctggccga ctatatactc
     8701 ccagaaccgg tcctggagcc ggaagcggcg ggtgcggcgc ggtgagcggc atagatgcat
     8761 ccttgacgtt ttcgggcgat tttgtttttt aatctagctg cgtaaaaagg gtgtataatc
     8821 tggctgggcg gtgcgcctgg gctagcgccg ttgcctcgcg atcgcgagat atgaacgcgt
     8881 atttgggcgt attgatgatc gcgctatccg gcctgggggc cactgcggaa gaccaggcgc
     8941 tccgccaacc tccgttctgt ggaaacctac agaaacacca ggttctccgt actcaagttt
     9001 ccgcgcccgg ggtcctcgct aagaataaag cgagaatcta tatgtactgg agaggggccc
     9061 gcttgcgatg gctgggaagg cagtgcaagg tggcgctgaa tggaacgtgg gtcgccgtgc
     9121 tatcccctaa cacgtactcc gtgatcgatg cggaggaagg caacctgaaa ttttgcggcg
     9181 cgagcaacgt tctgtcgcct acgtggagga gcctgttgtt tctgcacgcc acaggtggcg
     9241 agacctattt cgtcgaatgc agtccgggag gcgatgcttg gggtgccagc gatccgaccc
     9301 tgtccgagac ggaacaggag gaaagctgga aagcggtctc ccggctccgt gtcgtaacct
     9361 tccggttgaa atcaccgagg cgaactgaat gagtaccggt gtgttcctct tcgttgtagc
     9421 agaagttatt gcgccgttgt ccgccgggca cccggaagaa actccgtccg gcgaatatac
     9481 ctggaagaac gttgtttatt tgggcggcgt gcctggggtg agggtcgacc gcggcgattt
     9541 cgaccagact ctgacggtga ctccattgaa gctcatcgta atagtgcagc ctcccccgga
     9601 tttagacgct tccaagctcc cgcgaaaaag accgccaaga gtgctgttcg agatcccccg
     9661 cgacagcata atcggggtca attacgaagg attccggcac gatatgccca ttgctcaaat
     9721 atggttcgga atcccgagat actggccgaa agccaccgac cacctgcttg tcctaactta
     9781 ccggctctgc gacggacgag aaaccgaagt gttgctgcgc gtcgataaaa accatttccg
     9841 cgagatcctg caagccttgc gaaattaggt gcggcatggg ggacaaccgc ttcctggagc
     9901 tggaagtggt gagtgcgtcg cgctgaggtt gggggatcgc cccagaggcg ttaactgaca
     9961 agcccggctt ttgtatgtgc cgacgaaacc ggccaccatt tggtgcgact tccgcgtagt
    10021 atcctactgc agtaggatac tttgtatgcc acgcggcaac ccatcaccga agcttgctat
    10081 cactgtagac cctgacattc acgaaagcat ccttcgggcg gctgcccggg agggggtgag
    10141 cgtctccgcc tggatgaccg ctgcggcgcg cgaggcactg cgacagcggg ctggtttggc
    10201 ggcggtcgcg ctgtgggaaa agcagtacgg ccgcctcact acagaagaga tgaatgaagc
    10261 tcgccgcagt gtgcgggcac aactccgagc atcgcgaacg gtccggcgtc cggcatgagt
    10321 gctgttgtct acgacgtcgc cgttctcgtg gccgccgata aaaatgagcg gcgagcttgg
    10381 gctgaacaca aggtccgtct ggaactgggc gttgttccat tggttccggc accagtggtc
    10441 gcccaagtca gccggtcccc acaacaagct cagctccggc gtttcctgac tgggtgcgtg
    10501 gtcgttcctc tgagggagtc cgaagcgcat gaggccggac gcctgttagg catgaccaag
    10561 acggcggatg tcgttgacgc tgttgtggtg acgacagccc ttcggcaaaa agctacgatc
    10621 ctcacgagcg atccaggcga tatagaacgg cttgtgagag cctctggccg cgaagttgct
    10681 gtcgtcgcta tctaaggcaa cgcgaaaaca acctgtccag ttgtgctcgc cggcgccggt
    10741 acccggcttc cggaaaatgc tgccgggaac gggagaatgc aacccgtagc gttgcctatg
    10801 atgccgccga gaacagtacc gctggtccct gttgtggtgc tggtgaaaaa ggcaggtgcc
    10861 gtcgtgattt caccggccag attgctgctt tgggcttgcg aaatgacaga gaccagccgc
    10921 aaagcgtctt cgagagtctt cagtctcgct ttttgattct ggtcgaggat gtccacattg
    10981 cgcgtctggt attggtttgc ctgctctttc atttgacggc agatcatctc aatttctgca
    11041 tagcgtatgc caagggcgtc cacgctgagc ggatccttcg cggtttcctc aacgatttcg
    11101 ctttggactt gccagatacg gttctgcttc ccgatggacc actgattgta ttcgtcgttg
    11161 tttgccagga tcgtttgcac ctggccgtca gtcaactgga ggtattcttt tacctggccg
    11221 agcgtttgat cgaaccatcg attggtttgt gtgaatgcgg gggttaccaa aagaacacta
    11281 acagcccaaa taaagagctt catgccctca tggtacgtat gagtgcggtg gttggtttca
    11341 tcgggttcgc ctatattacg tgacgtcggg cctgttccgg aacgcccgcg cacaggccgc
    11401 aacaatgcga agtccgtcct cttgagagcc ggtgtcgaat tgatcacctt catgatcgac
    11461 tgcgttgctc accatggcga cacagccgac cgggacgccc cgcgcctttc cgaacgcgaa
    11521 cagagacgcc gcctgcatct cgaccgcgac aacgccctca tcggcccagt gctgcaactg
    11581 cgccctggtc tcgcggtacg gagcatcggt cgtccacacc ttgccggagc ggacggccca
    11641 acctgttgcc ggtagttcct tctccagcag cgccggtatg ggatcggggc acgacacctc
    11701 cgcgccgggt ggcaggtaat gaagactggt cccttcatcc cggacagcgg ctgtcacaac
    11761 taccagacaa gggagcggca actccggcga gacccgtccg gcggaggtta agccgatgat
    11821 ccatccggca cctgtcgccg cgagttgttc ggcaaccaac acagcgtacg ggccgccaat
    11881 tgtccgcgcg acgatgccgc aggtcatccc ttcactctcg acggcgtaca tcgtcgtatg
    11941 aaagcacggc cagcttggga ccggtttggc acggccggta tggaccagcc agtccgtgag
    12001 atctccgtca aactccagaa tgcacagcgg aggcacagtt ccacccggta tcatcctcat
    12061 gctgcgcacg ctgtgcatca gtgcatctgc ggtaaaggcc gagggttcgt cgagcggatg
    12121 attcagaatc ggaagcgtcc ggctatcggg catccgggct ttctccgctt atatccgact
    12181 ccagccggca atagcagtac aaaaattgct gcgttgtgcc gaacggcgtc tcgtgcgtct
    12241 cctccgagct ttccacgagc cggaaacggc tgccaaactc acggtgcagt tcgggagcgc
    12301 tgtatctcat cacgtcgaga
//
LOCUS       DASTMK010000461         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_504482, whole genome shotgun sequence.
ACCESSION   DASTMK010000461 DASTMK010000000
VERSION     DASTMK010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504482"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFQ79_19425"
     CDS             complement(<1..688)
                     /locus_tag="VFQ79_19425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0141903.1"
                     /translation="MRYAALQQRLPRFLWRHILAFEAAIGDAVEAFASRRPAGARVLD
                     AGAGESVHAKYFTHCRYTAVDLGIGDAAWNYAALDTMADLAALPFRPASFDACLNVVT
                     LEHVPEPGRVLDEIARTMKPGAPLLMIVPHEWEVHQQPHDYFRYTRFGMEYLLAKSGF
                     SSIEVTPVGGFFRLLSRRLLNGLQFFRGVWILPAALVLAPPALLLPFFDSLDRERNFT
                     LGYICTAKRRS"
     gene            complement(685..1659)
                     /locus_tag="VFQ79_19430"
     CDS             complement(685..1659)
                     /locus_tag="VFQ79_19430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0141904.1"
                     /translation="MSHTETTNTTPVCPACDGREFEERFQLTDRLYHTTREPFSILEC
                     KVCGLLRLYPWPDAAVLDRYYPPGYWFSPQPDTAHRLGEAYRRLVLRDHVRFVRGALR
                     HSGARGLVLDVGCGGGLFLHLLHEEGFPVAGLDFSLEAASVAWKRNGVPAVCANLGNA
                     PVSPEACSAVTMFHVLEHVYDPAAYLECAHRMLKPDGRLIVQVPNAECWQFRLFGSRW
                     NGLDVPRHLINFRRCDILRLIEFAGFEIVREKHFSLRDNPAGFATSISTALDPMARRV
                     RGVKEGGAVRLAKDLMYFGLVVAGLPFAVLEALFRAGSSIMIEARKIP"
     gene            complement(1659..2720)
                     /locus_tag="VFQ79_19435"
     CDS             complement(1659..2720)
                     /locus_tag="VFQ79_19435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0141905.1"
                     /translation="MNVLREPAPSASVAVIVVNWNRRELLRACLDSLAKQRGVSFEVV
                     LVDNGSSDGSVEMARRDFCENGRLRIRFIENRENRGFCAANNQGFAATDTEFIALLNN
                     DAEAGENWLASLLSAFEGRPDVGMAASKILVYEDPGRIDKAGHVIYLDGQNRGRGSGE
                     KDSGQYDRMEEVLWPDGCAAMYRRTMLDQIGGFDEDFFAYADDADLGLRGRIAGWKCL
                     YMPAAVVRHHRGSSLGLLSSRRLELIERNRVLFSVKLFPWSLLWLNWAFYFARLCAGI
                     WAAARNRGEAARFPGPAGKLTIALAMLKGDWQAAKLLPGTFKKRREVEKFRKLSPREV
                     RRLLLRHRVSLKELSEQAI"
BASE COUNT          619 a          908 c          823 g          485 t
ORIGIN      
        1 aggagcgcct tttggcagtg catatataac ccagtgtaaa gttgcgctcc cggtcgagcg
       61 aatcgaagaa cggcagtagt aaagcaggcg gggccagcac cagcgccgcc ggtaaaatcc
      121 agaccccccg gaaaaattgc agaccattca acaagcgccg ggaaagcagc cggaaaaagc
      181 ctcccacggg ggtcacttca atactggaga aaccggattt cgccaacagg tactccatgc
      241 cgaagcgcgt gtaccggaaa tagtcgtgcg gctgctggtg tacctcccat tcgtgcggaa
      301 cgatcatcag tagcggcgca ccgggtttca tcgtacgcgc gatctcatcc aatacgcggc
      361 ccggttctgg cacatgctcc agcgtcacca cgttgaggca ggcgtcgaaa ctcgcggggc
      421 ggaaaggcaa cgccgccagg tccgccatcg tatcgagcgc cgcatagttc catgcggcat
      481 cgccgatgcc gagatccacc gccgtatagc ggcaatgcgt gaagtatttc gcgtgtacgc
      541 tctcccccgc cccggcatcc aggacgcgcg cgccggcggg cctccgcgaa gcgaacgctt
      601 ccacggcatc gccgatcgcc gcttcgaacg caaggatgtg gcgccaaaga aatcgtggaa
      661 gacgctgctg gagcgccgca tacctcatgg tattttccgg gcttcgatca tgatgctcga
      721 tccggcgcgg aacaaggctt caagtacggc gaacggcaac ccggccacca ccaggccgaa
      781 gtacatcagg tctttcgcca accgtaccgc gccgccttcc tttacacccc gtacgcgccg
      841 ggccatggga tcgagagcgg ttgaaatgct ggtcgcaaag ccggccggat tgtcgcgcag
      901 cgagaagtgc ttctcccgta cgatctcaaa accggcgaat tcgatcagcc gcagaatatc
      961 gcagcggcgg aagttgatga gatggcgcgg cacatcgagg ccgttccagc gactgccgaa
     1021 cagacggaat tgccagcact ccgcattcgg cacctgcacg atcagccgcc cgtcgggctt
     1081 cagcatgcga tgcgcgcact cgagataggc ggcggggtcg tagacatgct cgagcacatg
     1141 aaacatcgtg actgcggagc aagcttccgg cgacaccgga gcattgccga ggtttgcgca
     1201 gaccgcgggc acaccgttcc gcttccaggc cacggaagcc gcttcgagtg aaaaatcgag
     1261 acccgcaacc gggaagcctt cctcgtgcag caggtgaaga aacagccctc ccccgcatcc
     1321 gacatccagc accaggccgc gcgcaccgga atgccggagc gcgcctcgga caaaccgcac
     1381 gtgatcccgc agcacgaggc ggcggtacgc ctctcccaat cgatgtgcgg tatccggctg
     1441 cggcgagaac cagtagcccg gcggataata acggtccagc acagcagcgt ccggccaggg
     1501 atagaggcgg agcaggccgc acaccttgca ctcgaggatg gaaaacggct cgcgcgtcgt
     1561 atggtacagc cggtctgtga gttgaaaccg ttcttcaaac tcgcggccgt cgcaggccgg
     1621 acatacgggc gtcgtgttgg tggtttcagt gtgggacact atatcgcctg ttcggaaagc
     1681 tctttcaagg agacacggtg acgaagcagc agccttcgga cctcgcgtgg actaagtttt
     1741 cggaacttct cgacctcccg cctttttttg aatgtgcccg gcagtaattt cgcggcctgc
     1801 cagtcgccct tcagcatcgc cagcgcgatg gtgagtttgc cggcggggcc cggaaagcgt
     1861 gccgcttcgc cgcggtttct tgcggccgcc cagatccccg cgcacaagcg cgcgaagtag
     1921 aaggcccaat tcagccacag caggctccat ggaaacaact tcacggaaaa cagaacgcgg
     1981 tttctttcga tcagttccag acgcctgctc gaaagcagcc cgagcgagga cccgcggtgg
     2041 tgccggacga ccgccgccgg catatacaga cacttccaac ccgcgatgcg cccgcgcagg
     2101 ccgagatccg cgtcgtcggc atacgcgaag aagtcctcat cgaaaccgcc aatttgatcg
     2161 agcatcgttc tgcggtacat ggctgcgcat ccgtccggcc agagaacctc ttccatgcgg
     2221 tcgtactgtc cggaatcctt ctctcccgaa ccacggccgc ggttctgccc gtccagataa
     2281 atgacgtgcc cggccttgtc gattcgcccg ggatcttcat agaccagtat tttcgacgcg
     2341 gccataccca catcgggcct gccttcaaac gcgctcagca acgaggcgag ccagttttca
     2401 ccggcttcgg catcgttgtt caacaaggcg atgaactcag tatccgtggc cgcaaagccc
     2461 tggttgttcg cggcgcagaa cccgcgattt tcccgattct caatgaagcg tattcgcaaa
     2521 cggccattct cacagaaatc gcggcgcgcc atctccacgg agccgtccga ggatccgtta
     2581 tccactagca ccacctcgaa cgatacgccg cgctgtttcg cgagcgaatc gagacaggcc
     2641 cgcaacaact cgcgccggtt ccagttgacg acaatgacgg cgacagatgc agatggagca
     2701 ggttctcgaa gaacgttcac accaagcctg acacgtaatt acgtattatc gcagagggtt
     2761 gcggaaaggc acgcggcgga ccggagaact cattccgcca tcagaatcaa cagtgcaagc
     2821 acagccagcg cagtg
//
LOCUS       DASTMK010000462         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_312130, whole genome shotgun sequence.
ACCESSION   DASTMK010000462 DASTMK010000000
VERSION     DASTMK010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312130"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1105
                     /locus_tag="VFQ79_19440"
     CDS             176..1105
                     /locus_tag="VFQ79_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEU0141906.1"
                     /translation="MIVTLTLNPAIDHNVLTDRLVFDDRGYILSTQESAGGRGINASR
                     VIHSFQGETLAIAPSGGKSGKRFESLLSCCGHPFRPVPIQHEIRTNLTITDEQGLTVK
                     LNERGAPMTKEELERIEQVVLEALDGAEWLMICGSVPPGVEPEFYSKLIQKANAMSVK
                     TLLDTDGDSLYEGVMAKPWVVTPNQQEAERLLATALLTRNHFFDAARRLLGMGSKAVV
                     LSLGSRGAVASADGKMFEVIPPRIDVVCPIGAGDALAAAFTWAMANGSSFADAVRWGV
                     AAGTASARLPGLNFASLEQARQDYAQVEVREVS"
     gene            complement(1106..>1514)
                     /locus_tag="VFQ79_19445"
     CDS             complement(1106..>1514)
                     /locus_tag="VFQ79_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141907.1"
                     /translation="EISETVSMAAEFPENYLAVFTLGYKAMRYNMFNDQLKQFHGSKA
                     RLDMGREWYEVYPESSAVDMKPSATKRLPGSFEPASRAHMRNFLECVRSRKEPTAPVE
                     AGQATNVTLCMAVEAQKTGRRVRFNPATKKMEV"
BASE COUNT          292 a          427 c          479 g          316 t
ORIGIN      
        1 gtgggttacc tatgtctact tcctatgggt ggcggtcgag gtcgtacgga tggcgattcg
       61 cagagagatg ccgcttgcat tccggctgtt tgcgatgatg tggaagagac acgtccgggg
      121 cggcaaggcc gccatcgaac aatgaccgcg caatggccca tttggagaac gagttttgat
      181 cgtcacccta acgctcaacc cggcgattga tcacaatgtt ctgacagacc ggctggtgtt
      241 tgatgaccgg ggctacatcc tgtcgacgca ggaatccgcc ggaggccgtg gtatcaacgc
      301 ttcccgggtc atccattcgt tccaaggcga gactctggcg attgctccct cgggaggcaa
      361 gtccggcaag cggtttgaaa gcctgttaag ctgctgcggg cacccgttcc gcccggttcc
      421 gatacaacac gaaatccgga cgaacctgac gattacggac gagcaggggt tgacggtcaa
      481 gctcaatgaa cgcggggctc ctatgacgaa ggaggagctg gaacggatcg agcaagtagt
      541 gctcgaagcg ctggacggtg cggaatggct catgatctgc ggcagcgttc ctcctggtgt
      601 ggagccggag ttctacagca agctgattca gaaggcaaac gcgatgagcg ttaaaacgtt
      661 gctcgatacg gacggagact ctctgtacga aggagtgatg gcaaagccct gggtggtaac
      721 gccgaatcag caggaagccg agcgcctgtt ggccacggcg ctcctgacgc gaaaccactt
      781 tttcgatgct gcccggcggc ttctgggaat gggttccaag gccgtcgtgc tttcgctcgg
      841 gagccgggga gccgtggcat cggctgatgg aaaaatgttc gaggtcatcc cgccgcgcat
      901 cgatgtggtg tgccccatcg gcgccgggga tgccctggcg gcagctttca cgtgggccat
      961 ggcaaacggc agcagcttcg ccgatgccgt ccgctggggt gtcgcggccg gaaccgcgtc
     1021 cgcccggctg ccgggactca acttcgcgtc cctggagcag gcgcggcagg attacgcgca
     1081 ggtggaggtc cgggaagtgt cctgatcaga cctccatttt tttggtcgca ggattaaagc
     1141 gaacgcgcct tccggtcttc tgagcttcca ccgccatgca aagcgtaacg ttggtggcct
     1201 gtcccgcttc gacgggtgcg gtaggctcct ttcgagaccg cacgcattcc agaaaatttc
     1261 gcatgtgcgc gcggctggcg ggctcaaaac tccccggcag tctcttggtg gcgctcggct
     1321 tcatgtccac agcgctgctt tccggataca cttcatacca ttcgcggccc atgtccaggc
     1381 gggcttttga gccgtgaaac tgtttcaact gatcgttgaa catgttgtac cgcatcgctt
     1441 tatacccgag cgtaaacacc gccaggtaat tctctggaaa ctcggcggcc atggagacgg
     1501 tttccgaaat ctcg
//
LOCUS       DASTMK010000463        16008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_407753, whole genome shotgun sequence.
ACCESSION   DASTMK010000463 DASTMK010000000
VERSION     DASTMK010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16008
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407753"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /gene="tuf"
                     /locus_tag="VFQ79_19450"
     CDS             <1..188
                     /gene="tuf"
                     /locus_tag="VFQ79_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU0141908.1"
                     /translation="RTTDVTGVAQLPEGTEMVMPGDNVNLEVELITPVAMDKGLRFAI
                     REGGRTVGAGTVTEILE"
     gene            207..359
                     /gene="rpmG"
                     /locus_tag="VFQ79_19455"
     CDS             207..359
                     /gene="rpmG"
                     /locus_tag="VFQ79_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU0141909.1"
                     /translation="MPRDIITFQCTECKRKNYTNTKNKKTTTERLELKKFCPFCRKHQ
                     MHREIK"
     gene            388..462
                     /locus_tag="VFQ79_19460"
     tRNA            388..462
                     /locus_tag="VFQ79_19460"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Trp,seq:cca)
     gene            574..810
                     /gene="secE"
                     /locus_tag="VFQ79_19465"
     CDS             574..810
                     /gene="secE"
                     /locus_tag="VFQ79_19465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU0141910.1"
                     /translation="MAEGNFLQRVSTWPSATKTYIGELNMEMRRVTWPSWKQVRATTL
                     VVIITVFLFAAYFRLVDLAVAAILNRVFSAFGSR"
     gene            818..1516
                     /gene="nusG"
                     /locus_tag="VFQ79_19470"
     CDS             818..1516
                     /gene="nusG"
                     /locus_tag="VFQ79_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU0141911.1"
                     /translation="MADQDENGIEKNGSAEVQNPPVPEPDTDVAEEPVAGEPEPEFSG
                     EAETPAAPSVEPSGKNWYIIHTYSGFEEKVKESLEGRARAFGFSDKIGQILIPKEEVV
                     ELRGGKKVTSKRLLYPGYVLVEMDMDDELWHAVKNTPRVTGFVGGGNTPVPLTPDEVN
                     NILYRQASSVERPRPKLTFEKNENVRIIDGPFTNFSGKVDEVNTERNTLRVLVTIFGR
                     ATPVELEFLQVEKI"
     gene            1520..1948
                     /gene="rplK"
                     /locus_tag="VFQ79_19475"
     CDS             1520..1948
                     /gene="rplK"
                     /locus_tag="VFQ79_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU0141912.1"
                     /translation="MAKKVTGQVKLQIPAGKATPAPPVGTALGPQGVNIMEFCKAFNA
                     KTSGKDQEGLIIPAVITIYQDRSFTFITKTPPVPVLIKRAVNLAKGSAEPNKNKVGTI
                     TKKQVEEIAKIKMPDLNCFDLAAAVRSVEGTARSMGVDVA"
     gene            2064..2771
                     /gene="rplA"
                     /locus_tag="VFQ79_19480"
     CDS             2064..2771
                     /gene="rplA"
                     /locus_tag="VFQ79_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU0141913.1"
                     /translation="MARKPGKKYESALKQVESRPYALEEAIPLVQKVKFTKFDETVEV
                     HMRLGVDPKHADQMVRGTVVLPNGLGKEKRVLVIASGEKIKDAEEAGADFVGGEDMVQ
                     KIQSEGWTDFDAVIATPDMMRSVGKLGKILGPRGLMPNPKTGTVTPDVAKAIKEVKAG
                     KVEFRVDKTGVIHAPVGRVSFESDKLLENASTLIQAVVRAKPPAAKGKYVRSVTICST
                     MGPGVPLDVALFNTKAG"
     gene            2783..3310
                     /gene="rplJ"
                     /locus_tag="VFQ79_19485"
     CDS             2783..3310
                     /gene="rplJ"
                     /locus_tag="VFQ79_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU0141914.1"
                     /translation="MKKKSQKQKDLEELKKQFASDGHVFVTGFEKLTVQQDFELRKSV
                     RNAGGSYKVIKNNLAEKASEGTPAEELFKDISGMTSLAHTATDPVALAKALTAYAKAN
                     PTFTFKAGIVEGRVIDVASIQDLASLPSREELLAKVLFLINSPAQRLVSAINGVGRNL
                     AVVLDQAAKEEKFKA"
     gene            3343..3720
                     /gene="rplL"
                     /locus_tag="VFQ79_19490"
     CDS             3343..3720
                     /gene="rplL"
                     /locus_tag="VFQ79_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU0141915.1"
                     /translation="MADINSIAEQIQGLTLLEASQLVKMLEERLGVSAAAATVAAAPA
                     AAAAAAPAAEEQTEFTVVLTAAGANKINVIKAVREVTSLGLKEAKDLVDNAPKPVKEG
                     INKEEAETIRKKFVDAGATVEVK"
     gene            3748..4095
                     /locus_tag="VFQ79_19495"
     CDS             3748..4095
                     /locus_tag="VFQ79_19495"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0141916.1"
                     /translation="MALQIEQTEIEPGVFVIALSGRMMLGSASQQLETLIPELLAQGH
                     RTFIFDLSGITHIDSTGIGKFIFAMNRIMQAGGRMYMAGATGQVRAGFAVTRLDTVFQ
                     FFPDVASARHALP"
     gene            complement(4110..5072)
                     /locus_tag="VFQ79_19500"
     CDS             complement(4110..5072)
                     /locus_tag="VFQ79_19500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU0141917.1"
                     /translation="MRRRDFLALGSVWFDTGCHIERVEDIVPPSPPPALRLLFGGDVM
                     LSRHVGRIARQKQDPSWPFRELAGTLKDADIAFVNLESPFSDKGRRTEKGMVFRAEPE
                     MISGLVHSGIDVVSTSNNHARDCGSYGVTFTLEWLAKHGILAVGTGATEEIAHAGVVL
                     ERKGVRFGFLAYTYDQLNGNYTDHDPRIAVMDLDRMKQNVQSMRAKADVIVVSMHAGA
                     EYRRTPHKSQVEFARAAINAGARIVVGHHPHVIQTVEEYGGGLIFYSLGNLVFDQSHR
                     RDTQKGLLAEVIFNSARLDGYYLLPVDIKQTVPRLAVKEDAMGS"
     gene            complement(5069..5800)
                     /gene="pgl"
                     /locus_tag="VFQ79_19505"
     CDS             complement(5069..5800)
                     /gene="pgl"
                     /locus_tag="VFQ79_19505"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU0141918.1"
                     /translation="MSARRYVYDTLEAAARACAKNIADVLEGALAGAPATTLAISGGS
                     TPLRMFEFLAGAAIDWARVHLFWVDERAVPPSDPQSNYLAAEQALIRPARIPPENVHR
                     IRAELPPEEAAEAYADEIRTYFHLKPDELPVFDVIHCGMGADAHTASLFPGEPLIEDG
                     ANIAAAVYVKKLAQHRITLLPGVLRKAAHVVFLVGGEDKRTALRAVFEAPYDPHEYPA
                     QLVARGGREVLWFLDRAAAGPADEP"
     gene            complement(5797..6792)
                     /locus_tag="VFQ79_19510"
     CDS             complement(5797..6792)
                     /locus_tag="VFQ79_19510"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEU0141919.1"
                     /translation="MTGAIHPEKVLQELERLWNDLGRNQGDEPGEVARACSMTLIVLT
                     DPATEREPASATLAELMREHPARAIIVRVDPRGTSSVEAGVSAQCWRPFGKRKQVCCE
                     QIEISVSRDSLPDAFTVLPALIAPDVPVVLWFRSGELMSVAPCETILPFAAKVLVDSN
                     ELGEPADALEQLERLSASVNVGDLNWTRVTRWRETIAQLFEMPEYVDRARLISHVTIR
                     HRTPETPAAAFYLAAWVRRSLSDQVAIEFQRVEGTSQGKTQSVVLSGADFSIELRRTG
                     AQCVEMTAGSMTSSTTFPSFSEWQLVSKELALLERDPIHRDVLKLAALMAREGVR"
     gene            complement(6792..8321)
                     /gene="zwf"
                     /locus_tag="VFQ79_19515"
     CDS             complement(6792..8321)
                     /gene="zwf"
                     /locus_tag="VFQ79_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU0141920.1"
                     /translation="MPSNPFHDPLRFEQKAPPCTVVIFGANGDLTRRKLAPALYRLAY
                     DRRIPPAFAVVGTSRTEMSDDQFRQKMKEAVQEFSEDGAFDEDVWRGFAPGLAYVAGD
                     INDPELYQRLDKCIEGVRESRQTSGNVLFYLSTQPSHYEPAVKGIGAAGLVKGGGWKK
                     LVVEKPIGHDLASARELDRIFHECFPEREIYRIDHYLGKETVQNILGFRFGNGIFEPL
                     WNRRYVTHVQITAAESIGVEGRGAFYQEAGALRDMIQNHLLQIMAVVAMEPPAAFEAD
                     AVRDERAKLLRSVRVYTPEEAAAHAIPGQYGPARVGGVDIPGFRQEPGVAADAQTETY
                     AAATFFVDNWRWAGIPFYLRSGKRLVRRVTEVAIQFNAAPLELFGPDEREAAYRAGPN
                     LLVLRIQPEEGISLKFSSKQPGRGMRMRGVSMDFNYATSFGARSPSAYETLLLDAMTG
                     YTTLYPRQDMVEASWTIVQPILDHWASTTFQFPNYPAGSWGPREANDMLARRGHEWRI
                     P"
     gene            8433..9227
                     /locus_tag="VFQ79_19520"
     CDS             8433..9227
                     /locus_tag="VFQ79_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141921.1"
                     /translation="MRRILAVALFLSAGAAIAFAQHRGGVRGGSLPYRTYGSPSGFGN
                     ILFPGTGTAPGVHMPPGNITDPSFAGRLGATVRGRPYYQGQTGGGLYGRRSRTTVVPY
                     AVPVMVGGYGYYPGPQEPNLTIVQSPPPAAPATPQVVINQYYTSDTAQPVMREYPEGS
                     LPETRSGKPSIYRAPGPPVVEESETAEQTKTAPRSADKATIYLIAFKDHSVYPALAYW
                     VEGDTVHYISMQHTHNQASLDLIDRELSSQLNSERNVEFRLPAAAP"
     gene            complement(9240..9641)
                     /locus_tag="VFQ79_19525"
     CDS             complement(9240..9641)
                     /locus_tag="VFQ79_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0141922.1"
                     /translation="MRRYGVVVVAALIWREGSLLIAQRKKDASHPLKWEFPGGKVERG
                     ETPREALERELEEELCIQANVGKEVIRYEYSYPGKAPLLLIFCGITEFHGEPQNLVFE
                     QIRWEKPANLPDYDFLDGDKDIVDRLARGGL"
     gene            complement(9650..10816)
                     /locus_tag="VFQ79_19530"
     CDS             complement(9650..10816)
                     /locus_tag="VFQ79_19530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0141923.1"
                     /translation="MPEWRNYFHLLGQRVRRRRFHSLFLFVTSRCNSLCRTCFYFDKL
                     NSKDDLTFEEIERISATAPPFEKLWISGGEPFLRDQLADILALFVNRNGVRNINLPTN
                     GLLPEKIFRTVDRVLALCPSVSIDLNFSLDGLANTHDAIRGVPNNFVRTIATMEQAEK
                     RYRGIRRLRRNVATVITRENYREIVDLGLELLERGSIDGQYFEVARGEMPDPGLKRMS
                     GEALADLHAKLMPLHRRYAERLFNHLPASFKLIAKAYYLGNIRLHFDLHEKCFERPAA
                     WPMPCTAGETSLVIDHNGKFRACELRNTVGDLHHFDFNLSAALASGELRAEIEEIPKA
                     GCWCTHSCFIQDSSKFSPRVQLFEIPWAYLRQRFSRFPELPLEELERFRSLDLA"
     gene            10858..11220
                     /locus_tag="VFQ79_19535"
     CDS             10858..11220
                     /locus_tag="VFQ79_19535"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU0141924.1"
                     /translation="MYDERLISHFRNPRNAGELEPPAVTVEVSNPACGDILRLSVLFE
                     NGRVRKAAYKTRGCTASIAAGSALTELVSGKTPAELRTLDATAIEEALGGLPAESKHA
                     AALCIDAVKAILKAAEKG"
     gene            complement(11285..12697)
                     /locus_tag="VFQ79_19540"
     CDS             complement(11285..12697)
                     /locus_tag="VFQ79_19540"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEU0141925.1"
                     /translation="MAEPRNSNPSGWNRRLLLIVGVTAAITLGVAALLVNIFERKQEA
                     RNPFFRVVELSDEIDDPAFWGKNFPLQYDSYRRTVDQERTRYGGSEALRHLPTKVDPR
                     SVVAQNKLQDDPRLKTMWAGYAFAVDFREERGHAYMLIDQKYTRRVLEFKQPGTCLNC
                     HASTYTTMRKLGNGDIFAGFDKMNQMTYAEASKQVAHPVSCIDCHDPQTMQLRITRPA
                     FIEGIRAYKASQGIQNFDVNKQASAQEMRSYVCGQCHVEYYFKGDEKRLTFPWEGGLK
                     VDDALAYYDRVGFRDWVHAETGAPMLKAQHPEFETFNQGVHAKSGVACADCHMPYMRE
                     GGLKISDHHVRSPLLNINRACQGCHHFSEQELKDRAETIQARFAHARNVAMDAVVELI
                     EDIKRAKESGKSDADLAAARDFHRKSQFFVDYVEAENSMGFHASGETLRVLTDAVDMA
                     RKGQLVLRGVAPSRWPAKKQ"
     gene            complement(12717..13181)
                     /gene="nrfH"
                     /locus_tag="VFQ79_19545"
     CDS             complement(12717..13181)
                     /gene="nrfH"
                     /locus_tag="VFQ79_19545"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HEU0141926.1"
                     /translation="MRNYMSNRGFSAPILLAALLGVMLGVGFFTFGYAKGSSYMTDDP
                     AACANCHVMREQYEGWMKSSHGKVAVCNDCHTPPGLVPKYATKAINGFFHSLAFTTGH
                     FPDEIQITRRNFNVTESSCLKCHEDIVTGIRATREHKADVECVTCHRTVGHM"
     gene            complement(13196..14161)
                     /locus_tag="VFQ79_19550"
     CDS             complement(13196..14161)
                     /locus_tag="VFQ79_19550"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0141927.1"
                     /translation="MRNAHLTLAAAAALLLLFGDGCNRSENKIIGVVPKGRAHLFWQS
                     VHAGAVKAAREAGVEVTWNGPPTETDYNVQLQIVDSMINRRVDAIVLAPIDRKVLVSV
                     VERANTQGIPVVIFDSPIDTENFVSQVATNNYAAGEMAAERMGEILKGKGEVVMVAVQ
                     PGAASTMDREKGFEDTIQKKFPGIRIIDKRYGMADFAKSLAVTENMLTAHPGLDGLFA
                     SNESSAVGAAQALKGRPGTKVKMVGFDWSPTLLEDVKAGVIDSLVVQDPFRMGYDSVK
                     AAIEKLNGGAPGKIQDLPPRLVTKENLNDPGIQQQLNPDLKKYLN"
     gene            complement(14371..15240)
                     /locus_tag="VFQ79_19555"
     CDS             complement(14371..15240)
                     /locus_tag="VFQ79_19555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0141928.1"
                     /translation="MNRRNFLQQATATAGAVALNAFPHHLFAAGTKKYATDRVKLGPT
                     GIQVSRLAMGTGTSGSGGSSNQTRKLGLQGMADLFKAAYGQGVTFWDSADQYGSHPHL
                     KLALKSVPREKVAILTKTHASTEKEMHADLDRFRRELGTDYIDVLLLHCMMDDNWPDR
                     KKGAMAVISEAREKGIVKTHGVSCHTLGALKTAARTPWVQVDLARINPAGVAMDADPA
                     TVLAVLEQMKASGKGIIGMKILGAGKLRNRADECLQYALAQKVVDCFTIGSESRTEME
                     DLLQKIPAASVRA"
     gene            15531..>16008
                     /locus_tag="VFQ79_19560"
     CDS             15531..>16008
                     /locus_tag="VFQ79_19560"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0141929.1"
                     /translation="MRRSNLYPFLAVLLGVALMALPAWPQSSQGSVRGTVQDASQAVV
                     PGVTVVLTNTATGVELRTTSNEAGIYVFPSVLPGSYKVEAEFAGMQKFEAAFTVRTQV
                     SANIDVVFQPAGTQTLITVQDITPIVKTDAPALGHTLERTRIEQLPLNGRFIMNLMN"
BASE COUNT         3346 a         4722 c         4654 g         3286 t
ORIGIN      
        1 tccggacgac ggacgtgacg ggagtggcgc agttaccgga agggaccgag atggtaatgc
       61 ctggcgacaa cgtgaacctg gaagtggagt tgatcacccc ggtggccatg gacaagggac
      121 tgcgtttcgc cattcgcgaa ggcggccgca cggtcggcgc cggaacggtc acggagatcc
      181 tggagtaggc gagcgagtgg atttttatgc cgcgagatat cattacgttt cagtgcaccg
      241 agtgtaagcg gaaaaactac acgaacacga agaacaagaa gacgacgaca gaacgcctcg
      301 agctgaaaaa attctgcccg ttctgccgga agcaccagat gcatcgggag attaagtaag
      361 gtatcgttga gggtgatagc agaaagaagg ggcgtaggtt taacggcaga ccagcggtct
      421 ccaaaaccgc gagtgggggt tcgaatccct ccgcccctgc cagccttcgc ttcgagccgc
      481 ttgtaacggt tttcgagcgg atgtggtctc gatttccagg cagagatctg tcactgccac
      541 ccttggaaag gttctttcca gacacgaata gcaatggcgg aaggtaattt cttgcaaaga
      601 gtttccactt ggccgtcggc cactaaaaca tacatcggcg aactcaacat ggagatgcgc
      661 cgcgtcactt ggcccagttg gaagcaggta cgcgccacca ccctcgtcgt cattatcacg
      721 gtgtttcttt tcgcggctta tttccgtctc gtggatctgg cagtcgcggc aattttgaac
      781 cgggtcttca gcgcctttgg cagcagatag gattctcatg gcggatcagg acgaaaacgg
      841 catcgagaaa aacggcagtg cggaggttca gaacccgccc gtgccggagc cggataccga
      901 tgtagctgag gaaccagttg ccggtgagcc cgagccggaa ttttccggtg aagcggagac
      961 tccggcggct ccctctgttg agccgtccgg aaaaaactgg tatatcatcc acacatactc
     1021 aggtttcgaa gagaaagtta aagagtcact tgaaggacgg gcgcgcgcct tcggtttttc
     1081 cgataagatc ggccagatcc tcattcccaa agaggaagtg gttgagctac gaggcgggaa
     1141 gaaggtcacc agcaaacggc tcctctatcc cggttatgtc cttgtcgaga tggacatgga
     1201 tgacgaactg tggcatgccg tcaagaacac gccgcgcgtc accggctttg tgggtggcgg
     1261 caataccccc gtccccctta cccccgatga agtgaataat atcctgtacc ggcaggcatc
     1321 ttcggtggaa cgcccacgcc cgaagttgac gttcgagaag aacgaaaacg ttcggatcat
     1381 cgacgggccg ttcaccaact tctccggcaa ggtggacgaa gtgaatacgg agcgcaacac
     1441 gttgcgcgtg ctggtcacta ttttcggccg tgctacgccg gtcgaactcg aatttctaca
     1501 ggtggaaaag atttagcata tggcgaagaa agtcactgga caggtaaagc tgcagatccc
     1561 ggcgggaaag gcgaccccgg caccgcccgt gggtaccgcg ctgggcccgc agggtgtcaa
     1621 tatcatggag ttctgcaagg cgtttaacgc caagacctcc ggcaaagatc aggaaggcct
     1681 gattatcccg gctgttatca cgatttacca ggaccgctcc ttcactttca tcaccaagac
     1741 gccgccggtg ccggttctca tcaagcgtgc ggtgaatctc gccaaaggtt cggcggaacc
     1801 caacaagaac aaggtgggga caatcaccaa gaagcaagtg gaagagatcg ccaaaatcaa
     1861 aatgcccgac ctgaattgtt tcgatcttgc cgccgctgtg agatcggtgg aaggcacggc
     1921 tcgaagcatg ggagtggatg tcgcctgacg gctgcgcgtt gcggcgggtg accgctgttt
     1981 gttagagtaa gaagttcggt tacaaacgaa cttaatggga ggcaaggttc ctgacgcctt
     2041 gccgttcgca ccagtgagga agaatggcta gaaaacccgg aaagaagtat gagtctgccc
     2101 tgaagcaggt tgaatcccgt ccatacgccc ttgaagaggc catacctctg gttcaaaagg
     2161 tcaagttcac gaaattcgac gagaccgtcg aggtgcatat gcgcctgggg gtcgacccga
     2221 agcatgcgga ccagatggtt cgaggcactg tcgttttacc caacggactc ggcaaagaga
     2281 aacgcgtcct ggtaatcgcc tcgggcgaaa agatcaagga cgccgaggaa gcgggagcgg
     2341 atttcgtcgg cggcgaggac atggttcaga aaatccaatc cgaaggctgg acggatttcg
     2401 atgccgtaat cgccacaccg gacatgatgc gctcggtcgg aaagctcgga aagattcttg
     2461 gcccgcgcgg gctgatgccg aacccaaaga ccggcaccgt cacgccggat gtggccaagg
     2521 cgatcaagga agtcaaagcg ggaaaagtgg aatttcgtgt cgacaaaacg ggcgtcatcc
     2581 acgccccggt cggcagggtc agcttcgaga gtgacaaact gctggagaat gcatctacgc
     2641 tgatccaggc ggtggttcgg gcaaagccgc cggcggccaa agggaagtat gtccggagcg
     2701 tgaccatctg ctccactatg ggccccggtg ttccgctcga tgtcgccctc tttaacacca
     2761 aggccgggta ggcgaggcaa gtatgaaaaa gaaatctcaa aaacagaagg atctcgaaga
     2821 gctgaaaaag cagtttgctt ccgacgggca tgtgttcgtc accggcttcg agaagttgac
     2881 cgtccagcag gatttcgagc tgcgcaagag cgtcaggaac gccggcggga gctataaggt
     2941 gatcaagaac aatctcgcgg agaaggcttc cgaaggaacc cccgcggagg agttgttcaa
     3001 ggacatctcc ggcatgacct cccttgctca taccgcgacc gacccggtgg ctctggctaa
     3061 ggcactgacc gcttacgcaa aggccaatcc gactttcacc ttcaaagcgg gcattgtgga
     3121 gggccgggtt atcgacgtcg cgtcgattca ggacctggca agccttccgt cccgtgagga
     3181 actgttggca aaagtgttgt tcctgatcaa ttcgcccgcc cagcgtcttg tcagcgccat
     3241 caatggcgtg ggacgcaacc tggctgtggt tctcgatcag gcggcgaaag aagagaaatt
     3301 caaggcataa gaagaaagat ctgacaggag ttaaagagag aaatggcgga tatcaacagt
     3361 atcgcggaac agattcaggg gctgacactg ctcgaggcat ctcagctcgt caaaatgctt
     3421 gaagagcgcc ttggcgtttc ggcggcggca gccactgtgg cagccgcacc ggccgctgcc
     3481 gcagcagccg caccggccgc ggaagagcag accgagttta ccgtcgtgct aacagccgcg
     3541 ggtgccaaca agatcaatgt catcaaggcc gttcgcgagg tcaccagcct cgggctcaag
     3601 gaagccaaag acctggtgga caatgcgcca aagccggtaa aagaaggcat caacaaggaa
     3661 gaggcggaga ccatccggaa gaagttcgtg gatgccggcg ccaccgttga agtgaaatag
     3721 caagtttgct tcctgtgaag atcgacggtg gccctacaga tcgagcaaac cgagatcgaa
     3781 ccgggtgtgt tcgtcattgc cttgtccggc cgtatgatgc tcggctccgc aagccagcaa
     3841 ctcgaaactc tgattcccga gttgctggcg caaggccacc gcacgttcat ttttgatctt
     3901 tccggtatta cccacatcga cagcaccggc attggaaagt tcattttcgc catgaacagg
     3961 attatgcagg cgggcggcag aatgtacatg gctggtgcca ccggtcaggt gcgggccggg
     4021 tttgccgtca cccgcctgga taccgtcttc cagttcttcc ccgacgtggc ttcagcacgc
     4081 cacgcccttc cctgacagcc ccggaccggt caggaaccca tcgcatcttc tttcactgcg
     4141 aggcgcggta ccgtctgttt gatatccacc ggcaaaaggt agtatccgtc gagtctcgcg
     4201 ctgttgaaaa tcacttccgc gagcaatccc ttctgcgtat cgcggcggtg ggattgatcg
     4261 aacacaaggt tgcccagcga gtagaagatc agaccgccgc cgtattcctc caccgtctga
     4321 atgacatggg gatggtgccc taccacgatg cgtgcgcccg cgttgattgc agctctggca
     4381 aactccacct ggcttttgtg cggcgtcctc cggtattccg ctccggcatg catggagact
     4441 acgatcacgt cggctttcgc ccgcatgctt tgaacgttct gcttcatgcg atcaaggtcc
     4501 atcaccgcga tgcgagggtc gtggtcggtg tagttgccgt tgagctgatc gtaggtatag
     4561 gcaagaaagc cgaaccgcac acccttccgc tccagcacca cgccggcatg tgcaatctct
     4621 tcggtcgcgc ccgttcccac cgctaaaatg ccgtgctttg ccaaccattc gagcgtaaac
     4681 gtgacgccgt aggagccgca gtcgcgcgca tggttattcg acgtggaaac cacatcgatt
     4741 cccgagtgga ccagtccgga gatcatttcc ggctccgccc tgaacaccat tcctttttcg
     4801 gtgcgccggc ctttatccga aaatggcgat tcaagattga cgaacgcgat atcggcgtcc
     4861 ttcagcgtcc cggcgagttc ccgaaacggc caggagggat cctgcttctg ccgcgcaatc
     4921 cttccgacgt ggcgtgacaa catgacatcg ccgccgaaga gaagccgcag ggccggcggc
     4981 ggtgaaggag gtacgatgtc ttccacccgt tcgatatggc agcccgtgtc gaaccatacg
     5041 ctgccaagag ccaggaagtc ccgccttctc atggttcgtc agccggtccg gcggcagcgc
     5101 ggtccagaaa ccacaacacc tctcgtccgc ctcgggccac caactgggcc ggatattcgt
     5161 gcgggtcgta gggagcctcg aacacggccc gcagcgcagt cctcttgtcc tcgccgccta
     5221 ccagaaacac gacatgcgcc gctttccgca acacgccggg cagcagagta atccggtgct
     5281 gcgcgagctt cttcacatag accgcggcgg cgatattcgc gccgtcttca atgagcggtt
     5341 ctcccgggaa aagacttgcc gtgtgcgcat ccgcccccat gccgcaatga atgacgtcga
     5401 agacaggcaa ctcgtccggt ttcaaatgga agtatgtccg gatctcatcg gcgtaagctt
     5461 cggccgcctc ctccggcggt agctcagcgc ggatgcgatg aacattctcc ggcggaatcc
     5521 gcgctggacg aatcaatgcc tgctccgccg ccaggtagtt gctttgcgga tccgaaggtg
     5581 gaaccgcgcg ttcatctacc cagaacaggt ggacgcgcgc ccaatcgatc gcggcgccgg
     5641 ccaggaattc gaacatccgc aggggcgtgc tgccgcccga aatcgccaga gtagtcgcag
     5701 gcgcaccggc caatgcgcct tcgagcacgt cagcgatgtt tttcgcgcac gcccgggcag
     5761 cggcttccag cgtgtcgtac acataacgcc gggcgctcat cgcactcctt cgcgggccat
     5821 gagcgctgct agcttcagaa cgtcgcggtg aatcggatcg cgctccagca gcgccagttc
     5881 ttttgaaacg agttgccact cgctaaatga aggaaaggta gtgctcgagg tcatcgaacc
     5941 cgccgtcatc tcgacgcact gtgcgccggt gcgcctcaac tcaatggaaa aatccgctcc
     6001 ggacaatacc acgctttggg tcttgccttg tgaagttcct tcgacgcgct gaaactcaat
     6061 cgcgacctga tcggaaagcg agcgccggac ccacgccgcc agatagaaag ccgcggccgg
     6121 ggtctcgggc gtgcgatggc gtatggtaac gtgcgagatc agccgggccc gatccacata
     6181 ctcgggcatt tcgaacagtt gagcgatggt ctcgcgccac cttgtgacgc gcgtccaatt
     6241 cagatctccc acgttcaccg acgcgctcaa ccgctccagt tgttccaagg catcggccgg
     6301 ctcgcccaac tcgttcgaat cgacaaggac cttggcggca aacggcagaa tggtctcaca
     6361 gggagcgacg ctcatgagtt ctcccgaccg gaaccatagc acgacgggca catcgggagc
     6421 gatcaatgcg ggcagcaccg taaaggcatc cggcagcgag tcgcggctga cggaaatctc
     6481 tatctgctcg cagcagacct gcttccgctt gccgaaaggc cgccaacact gcgctgaaac
     6541 accggcttca acggatgacg tgccgcgggg gtctacgcgc acgatgatgg cgcgggcagg
     6601 gtgctcgcgc atcagttcgg ccagggttgc actcgccggc tcccgttccg tcgcgggatc
     6661 ggtaagcaca atcagcgtca tggagcaggc gcgggccact tctcccggct cgtcgccctg
     6721 attcctgccc agatcgttcc acaagcgttc gagttcctgg agtacttttt ccgggtggat
     6781 ggcgccggtc atcagggaat cctccattca tgcccgcgcc gcgcgagcat gtcgttcgct
     6841 tcgcgcgggc cccaacttcc cgccgggtag ttcggaaatt gaaaggtcgt ggacgcccag
     6901 tggtccagga taggctggac gatggtccaa cttgcctcta ccatgtcctg ccgcgggtat
     6961 agcgtggtat atcctgtcat cgcatccaac agcagcgtct cataggcgga aggagagcgt
     7021 gcgccgaaac tcgtggcgta gttgaaatcc atcgacacgc cgcgcattcg cattcccctg
     7081 cccggctgct tcgacgagaa tttaagcgaa atgccttctt ccggttggat gcgcaaaacg
     7141 agcagatttg gaccggcgcg gtaggcggcc tcgcgttcgt cggggccgaa gagttcgagc
     7201 ggcgcggcgt tgaattgaat ggcaacttcc gtcacccgcc ggaccaggcg cttcccggac
     7261 cgcaaataga agggaatacc ggcccagcgc cagttatcca caaagaacgt agcggcggca
     7321 taagtctccg tttgggcatc ggctgctacg ccgggttcct gccggaatcc cggtatgtcc
     7381 acaccaccca cacgcgccgg accgtattgg cccgggatgg catgtgccgc cgcttcttcg
     7441 ggagtgtaca ctctcacgga gcgaagcagt ttcgcgcgtt cgtcgcggac ggcgtcggcc
     7501 tcgaaagcgg caggaggctc catggccacc acagccataa tttgcagcag gtggttctgg
     7561 atcatgtccc tcagcgcgcc cgcttcctga tagaaggcgc cgcggccttc cactccgatc
     7621 gactccgccg cggtgatctg gacatgcgtc acatagcgcc tgttccacag cggttcgaag
     7681 ataccgttgc cgaaccggaa gccgaggatg ttctgcaccg tctctttgcc gaggtaatga
     7741 tcaatgcggt agatctcccg ctccgggaag cattcgtgga agatgcggtc caactcgcgc
     7801 gcgctggcga gatcgtggcc gatgggtttt tcgaccacca gctttttcca gccgccgccc
     7861 tttaccagtc cggcggcgcc gatccccttg acggcaggct cgtagtggct cggctgcgtg
     7921 gaaagataga ataatacgtt gccgctggtt tgccgggact cgcgcacacc ttctatacac
     7981 ttgtccagac gctggtagag ttcgggatcg tttatgtcgc cggcgacgta ggccagcccc
     8041 ggagcaaacc cgcgccagac gtcttcgtca aacgcgccgt cttcggaaaa ttcctggacc
     8101 gcttccttca tcttctggcg aaactggtcg tcggacattt ccgtccgtga agtgcccacc
     8161 acggcgaagg ccggcgggat gcggcgatcg tacgcaaggc gataaagcgc aggcgccagc
     8221 ttgcgccggg tcagatcgcc gttagccccg aaaatgacga ccgtgcaggg gggcgctttt
     8281 tgctcaaacc ggagtggatc atggaacgga tttgacggca tgtcttctcc gtaggatact
     8341 ccgcaagtag ccgggcagga cggctcaagc cgccgtatcc cctgttgccg tgaacgattt
     8401 tcggcggtaa gcttcaggtt agggggtccc tgatgagacg gattctcgcg gtggcgttgt
     8461 ttctttccgc cggagcagct atagcgttcg cgcagcatag gggcggggtt cgcggaggaa
     8521 gtctgccgta caggacctat ggatcgccca gcggattcgg caacattctg tttcccggga
     8581 cgggcaccgc gcccggggtg catatgcctc ccgggaacat aacggacccg agcttcgccg
     8641 gccggctggg cgcgaccgtg cggggccgtc cttattacca ggggcaaacc ggcggtggtt
     8701 tatacggaag acgtagccgg acaacggtgg tgccttacgc cgtccccgtc atggtgggcg
     8761 ggtatggtta ttacccggga cctcaggagc caaacctcac catcgtacag agcccgcctc
     8821 cagcggcccc ggctacaccg caggtggtca tcaaccagta ctacacttcg gacacggcgc
     8881 aacctgttat gcgggaatat ccggagggct ccctgcccga aacccgatct ggcaaaccgt
     8941 ccatctacag ggctccggga ccgccggtcg tcgaggaatc cgaaacggca gagcaaacga
     9001 aaaccgctcc gcgttccgcc gacaaagcga ccatctacct gatcgctttc aaagaccact
     9061 cggtttatcc ggcattagcg tattgggtgg aaggcgacac ggtccactac atctccatgc
     9121 aacacactca caatcaggcc agcctggatc tgattgaccg ggagctttcc agccaattga
     9181 attcggagcg caacgtggaa tttcgacttc cggcagcggc accataagag acgctccctt
     9241 taaagaccgc ctcgtgccag ccggtccacg atgtccttgt cgccgtccag aaagtcgtag
     9301 tccggcaggt ttgccggctt ttcccatcgg atctgctcaa agaccaggtt ttgcggctca
     9361 ccatgaaatt cggtgatgcc gcagaagatc aagagcagcg gtgcttttcc cgggtaactg
     9421 tattcatagc ggatcacttc ttttcccacg ttggcctgaa tacagagctc ctcttccagt
     9481 tcgcgttcga gcgcttctcg tggcgtttcg cctcgctcca cttttcctcc cggaaactcc
     9541 cacttcagcg ggtgagacgc atctttcttg cgttgcgcga tcagcagcga cccctcccgc
     9601 cagatcagcg cggcaaccac tacgacaccg tatctgcgca tatcgccgct caggcgagat
     9661 ccaaagaccg gaatctctcg agttcttcga ggggcagctc cggaaaacgc gagaaacgct
     9721 gccggagata ggcccagggg atttcgaaca gttgtactcg cggcgagaac ttggaactgt
     9781 cctgaataaa acagctatgc gtacaccagc agccggcctt gggaatctct tcgatctccg
     9841 cgcgcagctc gcccgaagcc agcgcggcgg agaggttgaa atcaaagtgg tgaagatctc
     9901 caactgtgtt ccggagttcg cacgcgcgaa actttccgtt gtgatcgatg acgagagaag
     9961 tttcacccgc cgtgcagggc atgggccacg ctgccgggcg ctcgaagcac ttctcgtgca
    10021 ggtcgaaatg caatcggatg ttgccaaggt aatacgcctt ggcgattagc ttgaaagagg
    10081 cgggcagatg attgaacaac cgttcggcat agcgccggtg cagcggcatc aatttggcgt
    10141 gcaggtccgc aagcgcttcg ccgctcatcc gtttgagtcc cgggtccggc atctccccac
    10201 gcgcgacttc gaagtattgc ccgtcgatcg accctctttc cagcagttcc aacccgaggt
    10261 cgacaatctc gcgatagttc tcgcgcgtaa tcacggtggc cacattccgg cggaggcggc
    10321 gaatgccgcg atagcgcttt tccgcctgtt ccatggtggc tatcgtgcga acgaagttgt
    10381 tcggcacgcc ccggatggcg tcatgcgtat tggccaggcc atcgagtgaa aaattcaagt
    10441 cgatggacac ggacgggcac agcgccagca cccgatcgac ggtccggaaa atcttctccg
    10501 gcagcagccc gttggtggga aggttgatat tccgcacgcc gttccggttc acgaacaggg
    10561 cgaggatgtc cgccagctga tcgcgcagga aaggttcgcc cccggagatc cagagcttct
    10621 caaaaggagg cgcggtagcc gaaatccgct cgatctcttc gaaagtaagg tcgtccttgc
    10681 tgttcagctt gtcgaagtag aagcatgtcc ggcaaagcga gttgcaccgg gacgtgacaa
    10741 agagaaacag ggaatggaaa cggcggcggc gcactcgctg tcccagaagg tggaaatagt
    10801 tgcgccactc gggcattgtg ttttattgaa acacaagtag aggaggaggc gcccgcattg
    10861 tacgacgaac ggctgatctc acatttccgg aatccgcgca acgccgggga actggaaccg
    10921 cccgccgtga cggtagaggt ctccaacccc gcctgcggcg atattctgcg cctttccgtt
    10981 ctcttcgaaa acggccgtgt tcgtaaagcc gcgtataaaa cgcgtggatg caccgcctcc
    11041 attgccgccg gttccgcgct cacggaactg gtgtccggca aaacccccgc cgagctccgc
    11101 accctcgacg ccacggccat cgaggaggcg ctgggcggct tgccggccga atcgaaacat
    11161 gccgccgccc tctgcatcga cgccgtgaag gcaatcctaa aagctgctga aaaagggtga
    11221 aaaccgcttg cttgcgcgcg cggctccgtt ccgagccgtg accgtgaggg agtgggcccc
    11281 aaagctattg cttcttcgcc ggccaccggc tcggggcgac cccgcgaagc acaagctgcc
    11341 ctttgcgggc catgtccacc gcatcggtaa ggacgcgaag cgtctctccc gatgcatgga
    11401 aacccatcga attctccgct tcgacgtaat cgacaaaaaa ttgcgattta cgatggaagt
    11461 cccgcgccgc cgccagatcg gcgtccgatt ttcccgactc cttcgcccgc ttgatgtctt
    11521 cgatcaattc gacaacggca tccatcgcaa cattccttgc gtgggcgaag cgcgcctgaa
    11581 tggtttccgc gcggtccttc aactcctgtt cggaaaaatg gtggcatccc tggcaggcgc
    11641 ggttgatgtt gagcaaaggg ctgcggacgt gatggtcgct gattttgagg cctccctcgc
    11701 gcatgtaagg catatggcaa tcggcgcacg ccacgccgga cttggcgtgg acgccctggt
    11761 tgaaggtctc aaactcggga tgctgcgcct tgagcatggg cgctccggtt tccgcatgca
    11821 cccagtccct gaaacctacg cgatcgtaat aggccagcgc atcgtccact ttcaatccgc
    11881 cttcccacgg gaacgtcagt ctcttctcgt cccccttgaa gtagtactcc acgtggcact
    11941 gcccgcaaac atacgatcgc atctcttgcg cggacgcctg tttattgacg tcgaaatttt
    12001 gaataccctg cgatgcctta tacgcccgga tgccttcaat gaatgccggc cgcgtgatcc
    12061 gcaactgcat ggtttgcgga tcatggcaat cgatgcagga gacaggatgt gccacctgct
    12121 tggaggcttc ggcataggtc atctggttca tcttgtcgaa gccggcgaag atgtccccgt
    12181 tgccgagttt gcgcatggtg gtgtacgtgg aagcgtggca gttcaggcac gttccgggct
    12241 gcttgaactc gagtacgcgc ctcgtgtatt tctgatcgat cagcatgtag gcgtggccgc
    12301 gttcttcccg gaaatcgacc gcgaaggcgt agccggccca catcgtcttc agccttgggt
    12361 cgtcttgcag tttattctgc gcgacgacgc tgcgggggtc caccttggta ggcaggtgcc
    12421 ggagcgcctc cgatccgcca taccgcgttc tttcctgatc gaccgtcctc cggtaggagt
    12481 cgtattgcaa aggaaagttc tttccccaga acgccggatc gtcaatttca tccgacaact
    12541 ccacgacgcg gaaaaaagga ttgcgtgcct cctgctttct ctcgaaaatg ttcacgagca
    12601 acgctgctac gccgagggtg atcgcggcgg tgacgccaac gatcagcagc agccttcgat
    12661 tccatccgga cggattactg tttcttggtt cagccatatt ttcccttgtg gtgtacttac
    12721 atatgtccga cggtgcgatg gcaggttacg cattccacgt cggctttgtg ttcccgcgtc
    12781 gcccggatgc ccgtgacaat gtcttcgtga cacttcaggc aggacgactc ggtaacgttg
    12841 aagttcctcc tggtaatctg gatctcgtct ggaaaatgcc cggtcgtaaa agcgagggaa
    12901 tgaaaaaagc cgttgatcgc cttcgtagcg tactttggaa ccaggcccgg cggagtgtga
    12961 cagtcattgc aaacggcaac cttaccgtgg ctcgatttca tccagccttc atactgctcc
    13021 cgcatgacat gacagttggc gcaggcggcg ggatcgtccg tcatgtaaga cgaacctttt
    13081 gcgtaaccga acgtaaagaa cccgaccccc aacatcacgc ccagcagggc cgccagcagt
    13141 attggggcgg agaacccgcg gtttgacatg taattacgca ttcccgccca gtgtatcaat
    13201 tcaggtattt tttgagatcg gggttaagct gttgctggat gccggggtcg tttaaattct
    13261 ctttggttac aagacgcggc ggcaggtcct gtattttgcc gggggcgcct ccgttcaatt
    13321 tttcgatcgc cgccttcacg gagtcatagc ccatccggaa aggatcctgc accacgaggg
    13381 agtcgattac gccggccttc acatcttcga gcagggtggg gctccagtcg aatccaacca
    13441 tcttgacctt cgttccgggc cggcctttga gcgcctgggc ggcgccgacg gcgctggatt
    13501 cgttcgaagc gaaaaggccg tcgagtccgg ggtgcgcggt caacatgttc tccgtcacgg
    13561 ccagggattt tgcgaaatcc gccatgccgt agcgtttgtc gataatccgg attcccggga
    13621 atttcttctg gatggtgtcc tcgaaaccct tctcccggtc catggtggat gccgctccgg
    13681 gctgcacggc caccatcacc acctctcctt ttcccttcag gatctccccc atgcgctccg
    13741 ccgccatctc acccgccgcg tagttattcg tcgctacttg cgagacaaag ttctccgtat
    13801 cgatgggcga gtcgaatatt acgaccggaa tcccctgcgt gttcgcacgc tcgacgacgc
    13861 ttaccaacac ctttctgtcg ataggcgcca ggacaatggc atccaccctc cggttgatca
    13921 tcgaatcgac aatctggagt tggacattgt agtcggtttc cgtgggaggc ccgttccagg
    13981 taacttccac tccggcttcg cgtgcggctt tgactgcgcc ggcgtgcacc gattgccaga
    14041 acaggtgggc gcgcccctta ggcacgactc cgatgatctt attttcgctc cggttacatc
    14101 cgtccccgaa tagcagtagg agagccgcgg cggcggccag agtcaggtgc gcgttccgca
    14161 tgaacccata agcatatgcg gcgcgcgtta cagaatcaag aactcacggc tccggggtgt
    14221 gctgagccgc gcgcgtgaga agctgtgaag aaattgaatt agccacggat gaacacggat
    14281 aaacacagat aagtttcctg ttttcaatct gtgtttatct gtgtccatcc gtggctctga
    14341 atctttcaaa ccttcttaca ctcgcggcca tcaggcccgg acactggctg cggggatctt
    14401 ttgcagcagg tcttccatct ccgtgcggct ttcgctgccg atggtgaaac aatccaccac
    14461 cttctgagcg agcgcatact gaaggcactc atccgcacgg ttgcgcaatt tgccggctcc
    14521 caggatcttc ataccgataa tacctttgcc cgaggccttc atctgttcga gaactgccag
    14581 cacggtggcg gggtccgcgt ccatggcaac ccccgcggga ttgatacgcg ccaagtccac
    14641 ttgcacccag ggtgtcctgg cggccgtctt cagtgccccc agcgtatggc aggagacgcc
    14701 atgcgtcttg acgatgccct tctcccgcgc ttcggagatc acggccatgg cgcccttttt
    14761 ccggtccggc cagttatcgt ccatcatgca atgcaggaga agaacgtcaa tatagtccgt
    14821 tccgagctcg cggcggaagc ggtcgaggtc ggcgtgcatc tccttttccg ttgacgcgtg
    14881 cgttttggtc agaatggcaa ccttctcgcg ggggacgctt ttcaaggcca gcttcagatg
    14941 cggatggctc ccgtactggt ccgccgaatc ccagaaagtg acaccctggc cgtaggcagc
    15001 tttaaataag tcggccatgc cctggagacc gagcttgcgg gtctggttgg aacttccgcc
    15061 actgccgctt gtgccggtac ccatcgcgag ccgcgatacc tgaattcccg taggccccag
    15121 tttcacccgg tccgtggcat acttctttgt gccagcggcg aaaaggtgat gtggaaaggc
    15181 gttgagagct accgccccag ccgtagctgt cgcctgttgc aggaagttgc gccggttcat
    15241 aaccctccgt agttatgaac aggctagcac aggcatcggg atacgcgtgt agataccctc
    15301 cggcaatgcc tggtactatt tctgaacgta ccagtacacc ggcagaaagg tggcgcagac
    15361 ggaaagatcc tacttgtaat caataagata tgggttcaag gggcgacata cgcgggacgt
    15421 acgttgctag acaggataga gctattaatc ttctggttcg actttcagac ctgcttgcct
    15481 cttttctgat ataatttata cgctttggga aaggtggtca ctttttgaat ttgagacgat
    15541 caaacttata tccttttttg gcggtcctgc tcggtgtggc gctgatggcg ttgcccgcct
    15601 ggccgcaatc cagccaaggt agcgttcgcg gaacggttca agacgcgtcg caggcagtgg
    15661 ttccgggcgt taccgttgta cttaccaaca cggctacagg tgttgaactc cgcactacca
    15721 gcaatgaagc tggtatctac gtgttcccat ccgtacttcc cggatcttac aaagtagagg
    15781 cggaatttgc cggaatgcag aaattcgagg cggcctttac ggtgcgcaca caggtttcgg
    15841 caaatatcga cgtagtgttc caaccggccg gcacgcagac attgatcacg gtccaggaca
    15901 tcacgcccat cgtgaaaacg gacgcaccgg cattgggcca tactctcgaa cggacgcgta
    15961 tcgagcagtt gccgctcaac gggcgcttca tcatgaacct gatgaaca
//
LOCUS       DASTMK010000464         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_439583, whole genome shotgun sequence.
ACCESSION   DASTMK010000464 DASTMK010000000
VERSION     DASTMK010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439583"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFQ79_19565"
     CDS             <1..238
                     /locus_tag="VFQ79_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0141930.1"
                     /translation="AWMKDQGHRVTRESAMAKLFASEIAVRVADEALQIHGGYGFIKD
                     YPVEKFYRDVKLCTIGEGTSEIQKLVIARQLLKS"
     gene            238..1143
                     /gene="meaB"
                     /locus_tag="VFQ79_19570"
     CDS             238..1143
                     /gene="meaB"
                     /locus_tag="VFQ79_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU0141931.1"
                     /translation="MPEWARQIRSGNVRALARAASAVENRAPEADALLRELFPYTGNA
                     LVMGITGPPGAGKSTLVDGIIRLLRREEQRVGVIAVDPTSPYSGGAILGDRIRMQSHY
                     ADPGVFIRSMATRGHLGGLAPATTDMALLFDAAGMDVVIVETVGVGQDEVDIVRLADV
                     TAVVLVPGMGDDVQAIKAGIMEIAGVFLINKADQPGADRLEREIHAMLGLAHHDGPAP
                     LVVRVVASEGAGIAEALDAIRSVAGNRGGNERSEHIWRRRLLEMYRERLLNRIPAAEL
                     DGAARDVALRRKDPYSIVDEWLQRI"
     gene            1121..1537
                     /gene="mce"
                     /locus_tag="VFQ79_19575"
     CDS             1121..1537
                     /gene="mce"
                     /locus_tag="VFQ79_19575"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU0141932.1"
                     /translation="MNGFSGFKIDHLGIAVRSLEIALGFYRDQLGMQASEPETVPAEK
                     VKVSMLAAGGPRIELLEAAASDSVIARFIEKRGEGLHHVALSVPDLNAVVERLRSSGA
                     KILNEPRTGAGGHRYVFVHPSSTGGVLLELIQEHTN"
     gene            1534..2397
                     /gene="mtnP"
                     /locus_tag="VFQ79_19580"
     CDS             1534..2397
                     /gene="mtnP"
                     /locus_tag="VFQ79_19580"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEU0141933.1"
                     /translation="MKAQIGILGGSGLYSMPGFEAQEEISMDTPWGSPSDAYIAGQLA
                     GKPVAFLARHGRGHRLSPSELNFRANIYGFKKLGVERIISLSAVGSLKQEHRPLDFLL
                     PDQFVDRTKGRPSTFFGDGLVAHISFADPICPELADTVFQACGNEGVSAKKGGTYLCM
                     EGPAFSTKAESNLYRSWGMDVIGMTNLQEAKLAREAELCYVTVAMVTDYDCWHPEHDA
                     VTVNDIIANLTRNADNAAKVVKAAIAAMPEQRKCKCGSALSHALITDPQVVPAETRKK
                     LELLVGKYLPR"
     gene            2375..3628
                     /locus_tag="VFQ79_19585"
     CDS             2375..3628
                     /locus_tag="VFQ79_19585"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU0141934.1"
                     /translation="MANTSPGDLAGELLKYCLRGEAWPASVLDALLDCATSEDSETAR
                     EGSRALFGIVAERLSDLFEPALCDVYADLFSQVIVRALPELDAAELAARYRRIRQPRR
                     FKGDPDRIRNVFVLSRVTLGADVAVTSPLLRCALDTFPSAAVYLVGPRKNWEMFAGEP
                     RVRHLPVAYGRSATLAGRLQIWPDLREQLLAPDSIVIDPDSRLSQLGLLPLGREEDYF
                     FFESRSYGGDGSGALAVLAGRWAAETFGSLEPRPFVSPVPTSKFGDGPIITVSFGVGE
                     NPAKRIPDPFEGKLLRDLVNPDALVLVDRGAGGEESERVDRAVARAGVPPERLRAIDC
                     AFADFASLIARSQLYVGYDSSGQHVAAACGTPLVTVFAGFAAPRTFERWRPTGNGPIE
                     IIRAEDPDPARVLAGVFGAIRRLRR"
BASE COUNT          672 a         1088 c         1170 g          719 t
ORIGIN      
        1 ggcgtggatg aaagatcaag ggcatcgcgt caccagggag tccgccatgg cgaagctttt
       61 cgcctccgaa atcgccgtcc gtgtggcgga cgaagcgcta cagattcacg gcggctacgg
      121 tttcattaag gactaccccg tcgaaaagtt ctatcgcgat gtgaagctgt gtaccatcgg
      181 cgaaggcacc agtgaaatcc agaagctggt gatcgcaaga caactgctga agagctgatg
      241 ccggaatggg cgcgacagat ccgtagcggc aatgtgcgag ccctggcacg cgctgcgagc
      301 gccgtcgaaa acagggcgcc tgaggcagac gctctcctgc gcgaactttt tccttatacg
      361 ggcaacgcgc tggtaatggg tatcaccggt cctcctggcg ccggcaaaag tacgctggtc
      421 gatggcatta tccgtctgct ccggcgcgag gagcagcgtg tgggcgtcat tgccgtggac
      481 cccaccagcc cttattccgg cggcgccatt ctgggcgacc gtatccgtat gcagtcccac
      541 tacgcggatc cgggcgtgtt catccgttcc atggcgacgc gcggccacct gggcggcctt
      601 gctccggcta ctaccgacat ggcgctgctc ttcgatgccg ccggcatgga tgtcgtaatc
      661 gtagagacgg tcggcgtggg acaggacgaa gtagatattg tacggctcgc cgatgtcacc
      721 gccgtcgtgc tggtgccggg gatgggcgac gacgtccagg cgatcaaggc aggtatcatg
      781 gagatcgccg gcgtctttct gatcaacaaa gcggatcaac ccggcgcgga ccgcctggag
      841 cgtgaaatac acgcgatgct gggtctggct catcacgatg gccccgcacc tctggttgtg
      901 cgtgtagtgg cgtccgaagg agcgggtatc gctgaagctc tcgacgcgat acgctcggtt
      961 gccggaaacc gcggtgggaa cgagcgaagt gagcacatct ggcgccgccg cctgctcgag
     1021 atgtaccggg agcggctgtt gaaccggatt ccggccgcgg aactggacgg cgccgcgcgc
     1081 gatgtggcat tgcgcagaaa ggatccttat tcgatcgtcg atgaatggct tcagcggatt
     1141 taagatcgac caccttggca tcgctgtccg ttcgctcgaa atcgcgctcg gcttttatcg
     1201 cgaccaactc ggcatgcaag cgagcgagcc ggagaccgta ccggcggaaa aagtgaaggt
     1261 gtccatgctc gccgccggcg gaccgagaat cgaattgctc gaagcggcgg cttcggattc
     1321 cgttattgcc cggttcatcg aaaagcgagg cgaggggctg catcatgtgg cgctcagtgt
     1381 tccagatctc aacgcggttg tcgagcgcct ccgttcatcc ggagcgaaga tcctgaacga
     1441 gccgcgcacg ggcgccggcg gacaccggta tgtatttgtc catccgtctt cgacaggcgg
     1501 agtgctgtta gaactgatcc aggagcatac aaattgaaag cacagatagg aattctgggt
     1561 ggcagcgggc tctattccat gccgggattc gaagcgcagg aagagatctc catggatacg
     1621 ccttggggca gtccttccga cgcctacatc gccggacagc ttgccggcaa accggtggcc
     1681 tttctcgcgc ggcacgggcg cgggcatcgc ctgtcacctt ccgagctgaa tttccgggcg
     1741 aacatttacg ggttcaagaa gctgggtgtg gaacgcatca tttcgctcag cgccgtcggt
     1801 tctcttaagc aggagcaccg ccccctcgat ttcctccttc ccgatcagtt cgtcgatcgc
     1861 accaagggcc ggccgtcgac attcttcggc gatgggctgg tggctcacat cagcttcgcc
     1921 gatcccatct gcccggaatt ggccgatacc gtgttccagg cctgcgggaa cgaaggagtg
     1981 agcgcaaaga aaggcggtac ctatctctgc atggaagggc cggcgttctc gaccaaggcg
     2041 gaatcgaacc tgtatcgaag ctggggaatg gacgtcatcg gaatgacgaa cctgcaggaa
     2101 gcgaaactcg cgcgggaagc cgagctatgc tatgtgactg tcgccatggt gacggattac
     2161 gactgctggc atcccgagca cgatgccgtg acggtgaacg atatcatcgc gaatctcacc
     2221 aggaacgcgg acaacgccgc gaaggtggtg aaggccgcta tcgcagccat gccggaacag
     2281 cgcaagtgca agtgcggttc cgcgttgtcg catgcgctga tcacggaccc ccaggtggtc
     2341 ccggccgaaa cgcgaaaaaa actggagctt ctggttggca aatacctccc ccggtgacct
     2401 cgccggagaa ctgttgaaat attgcctgcg cggcgaggcg tggcccgcga gtgtgctgga
     2461 cgctctgctt gattgcgcca ccagcgagga ttccgaaacg gctcgcgagg gcagccgcgc
     2521 gctcttcggc attgttgcgg aacgcctctc cgatctgttt gagcccgctc tctgcgacgt
     2581 gtacgcggac ctgttttctc aagtcatagt gcgcgcgctg ccggaactcg acgcggcgga
     2641 gctggcggca aggtatcgcc gcatacgcca gccacgccgg tttaaaggag atccggaccg
     2701 catccggaac gttttcgttc tttctcgtgt gacgctgggc gccgacgttg ccgttaccag
     2761 cccgcttctt cgctgcgccc tcgatacttt tccgtccgcc gccgtgtatc tcgtgggccc
     2821 ccgcaaaaat tgggaaatgt tcgcgggcga gccgcgtgtg cgtcaccttc ccgttgccta
     2881 cggcagaagt gcaaccctcg ccggccgcct gcagatctgg cccgatttgc gcgaacagtt
     2941 gctggcgccg gattcgatcg tcatcgatcc ggactcgcgg ctgtcgcagt taggcctgct
     3001 tccattaggg cgcgaagagg attacttctt ttttgagagc aggtcttacg gcggggacgg
     3061 ttccggtgcg ctcgccgttc tggccggccg ctgggcagca gaaactttcg gcagcctgga
     3121 gccgcggccg ttcgtctcgc cggtaccaac ttcgaagttc ggcgatggcc cgattataac
     3181 ggtgagtttc ggcgtgggag aaaaccccgc caaaagaatt cccgatccgt ttgagggaaa
     3241 gctgctgaga gatcttgtga atcccgatgc gctggtcctg gtggaccgcg gtgcgggcgg
     3301 tgaagaatcg gagcgggtgg accgcgcggt ggcgcgcgcc ggtgtaccgc ccgagcggct
     3361 ccgcgccatc gactgcgcgt tcgccgattt cgcctcgctg atcgcgcgca gccaactata
     3421 cgtgggttac gattcgtccg gccagcacgt ggccgccgcc tgcggaacac cgctggttac
     3481 ggtgtttgca ggttttgcgg ctccccgaac cttcgagcgc tggcgtccga cgggcaacgg
     3541 acccatcgaa atcattcgcg ctgaagaccc ggacccggcg cgagtactcg ccggtgtttt
     3601 tggcgctata cgccggcttc gccggtaggc tacttgccgg gcccttctc
//
LOCUS       DASTMK010000465         7664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_566492, whole genome shotgun sequence.
ACCESSION   DASTMK010000465 DASTMK010000000
VERSION     DASTMK010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566492"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1799
                     /locus_tag="VFQ79_19590"
     CDS             309..1799
                     /locus_tag="VFQ79_19590"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU0141935.1"
                     /translation="MDIFPAIIQGGMGAGVSNWRLAQAVARTGQLGVVSGTALDQIFA
                     RRLQDGDPGGYMRRGLSRFPFPDMARRVWSAYFVLGGKALGTSYKTPPMHTKDSPREL
                     QELCIVANFVEVILAREGHGHPVGINYLEKVQMAHLPSIYGAMLAGTAYVLMGAGIPL
                     KSPGVLDRFTCHEPASYPLQITGAQEADDTTMTFVPREFMEREIPLLIRPRFIAIVAS
                     NTLASTMVKKANGRVDGFVIEGPTAGGHNAPPRGRLQLDEAGEPVYGPRDAVDLEKLR
                     ELGLPFWLAGSYGSPEKLRAAWSAGAAGVQVGTPFAFCAESGLRNDYKQAILNDIAAG
                     KAHVFTDSLASPTNFPFKVVQVSGSLSGSEVYAARPRICDVGYLREAYREPDGTIRYR
                     CAAEPVTTYVSKGGRLENTNGRKCLCNALLANIGLPQIRSGKYVENGLVTAGSSLADV
                     ARFLPENGLVYQAGDVIAKLLNVETLPALPRPEVCAGAALSDAGHC"
     gene            1868..3262
                     /locus_tag="VFQ79_19595"
     CDS             1868..3262
                     /locus_tag="VFQ79_19595"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HEU0141936.1"
                     /translation="MAAVILLAMPVWAQRRATGPLRVLQSNPRWFTDGSGKAVYLAGS
                     HTWWTLQDNDLLYPAKTNPPHRFDFDGYLDFLERHNHNFFRLWRWEPPKWTDSYDKKV
                     AYCTPHPWVRTGPGTAADGQPKFDLTRFNQEYFDRMRVRLTKAGDRGIYAAVMLFEGW
                     AMQFTDGWTYHPFNGANNINGVDADINCDGKGLEFNSLELGAMARRVLDLQEAYVRKV
                     IDTVNDLDNVLYEVSNEAFAGSTDWQYHMIRFVKQYESDKPKQHPVGMTFQYRGGANA
                     VLYASPADWISPNEGGSAESFRDNPCADCTTKVVVSDTDHLWGHTGGDSVWVWKSFCR
                     GLNVLFMEELLPSPTWQDSARLAMGQTRRYAGKMDLASMKPEPGIVRGGYCLAHRGSE
                     YLVFQPGVIGQFTVDLKDAPGFFDVEWLNVNKDETVAGKPVQGGSVVTFRTPFGGPAA
                     LYLKRSQGASAAGK"
     gene            3279..3638
                     /locus_tag="VFQ79_19600"
     CDS             3279..3638
                     /locus_tag="VFQ79_19600"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0141937.1"
                     /translation="MEQKPVAIDTSTVEWEERFNEKLGRSLFRKNLFEDAETGMEIRL
                     VRYPAGVVNPWHTHPCAHGMYVLEGTLATNAGTFGPGTFVWFPEGSVMQHGATAEADV
                     TVLFITNKPFRIDYVQR"
     gene            complement(3629..4669)
                     /locus_tag="VFQ79_19605"
     CDS             complement(3629..4669)
                     /locus_tag="VFQ79_19605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU0141938.1"
                     /translation="MMSAANAFVRGEFDIRGDMVAAVRFKRASTGSGGRPWFETLLLY
                     LRHLRTRLATRMRTTRDIRFHYDRSNDFYRLFLDSRMVYSCAYFRNSEWSLDRAQLEK
                     LDHICRKLDLQPGDRFLDIGCGWGSLLLRAAGSYGVLATGCTLSENQAKLACRLIEAR
                     RLGNSASVRLADYRKLTGPYDKIASVGMFEHVGPIHLEQYFRTVYRLLDNSGLFLNHG
                     IIRPEGVREDAETLFIDRYVFPGGGLSRLAQTVRTAERAGFELLDAENLRCHYGMTCR
                     AWLERLLANRNACLEAVDRQTYRTWLIYLAGSAANFEDGGADVFQLLFAKRTHPRPRR
                     MSRDHMYEPVQR"
     gene            4954..6603
                     /locus_tag="VFQ79_19610"
     CDS             4954..6603
                     /locus_tag="VFQ79_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141939.1"
                     /translation="MQLFLFRVSVLLAMAAAAGFSQSGDVLYNGIRLPAQWPPTGIKL
                     SHEPLPDPPYLASPPDIIPIDVGRQLFVDHFLIEETTLRRTFHQAEYFAGNPVLKPDK
                     PWELRRDGGGGAMPFSDGVFYDPHDRLYKMWYLGLGATLYATSKDGIHWDKPELDVEP
                     GTNVVHRGNRDSSTVWLDLEESDPQRRYKFLYNPGNMRPMILHFSSDGIHWGQPAAQS
                     VKVADRTTLFRNPFRKVWAYSIRDHTPGVISPQRMRRPGEHVRFRTYAESPDIAAGLE
                     WKPVPWVGADRLDAARVDLNARPELYNLDAVAYESAMVGLFSVWRGQFKDREKPNEIV
                     AGFSRDGFHWYRPSRRAFIPVSARHGDWNHGNVQSAGGVCLLVGDRLFFYVSGRSRAP
                     GVIGQNSTGLATLRRDGFVSMDAGAVEGTLTTRPVSFRGTRLFVNTNTEAGELTVEVL
                     DQKGRAIPAFSRAQCIPVRADNTAREVSWKNADLSSLSGKPVRFRFHLRHGKLYSFWV
                     SPDASGASYGYVGAGGPGFTGPTDTVGTGIYQHCCASEILH"
     gene            6629..>7664
                     /locus_tag="VFQ79_19615"
     CDS             6629..>7664
                     /locus_tag="VFQ79_19615"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0141940.1"
                     /translation="MKRRKLFGGSITCGVLLWAVHSTLLCAQESGRGQSLYNSLCMSC
                     HGGDGNGGERAPGIVRQVRARDDRQLAELVRQGIPSGGMPAYKLAEEPMRELIVFLRS
                     LRPARTRPARRAKVETIAGRALEGVVLNQSASEMQMRSDDGRLRLLRRTGDRYREVTS
                     QTDWLTYNGADGGNRYSTLAQITKKNVSRLAPAWVFPLSNAARLEVTPVVMQGVMYVT
                     AANECYALDAGSGRELWHYRRPRTPGVAGDAGGGINRGVAVSGERVFMVTDHAHLIAL
                     NRFTGALLWDTTMADYKENYGATSAPLVTGGLVVSGHSGGDEGVRGFLAAFDQATGKE
                     VWRFWTVPKPG"
BASE COUNT         1550 a         2134 c         2455 g         1525 t
ORIGIN      
        1 gccgcattat tcgcgatcaa tatgctggtt ggcacccagg caggttccag ctatagcgaa
       61 gtggaatata aacagtggct taccgaagcc ggattccgcg atttccgccg gccggaaccg
      121 gacggcgatc tgatcgtcgc aacgcgctag cgcgaccgct tgctggcgcg cgcggatcgg
      181 taagaccgtt gtttctgagc cgcgacggtc acggagcggt tgtagagctc attcgcacct
      241 gaatctcccg ttttgcggac agaagtatta caatccggaa cgcggatcac tgaaacaatg
      301 aagaattaat ggatattttc cccgcgatta ttcagggtgg catgggtgca ggggtttcga
      361 actggcggct ggcgcaggcc gtcgcccgga ccggtcagct tggggttgtt tccggaacgg
      421 ctctggacca gatctttgcc aggcggctcc aggacggtga tccgggaggg tacatgcgcc
      481 gcggcctcag ccgttttcca tttccggata tggccaggcg tgtctggtcc gcttattttg
      541 ttcttggcgg caaggccctc ggcacatcct ataagacgcc gcccatgcac acgaaggaca
      601 gtccgcggga gttgcaagag ctttgtatcg tggcgaactt tgtggaggta attctcgccc
      661 gggaaggtca tggacatccc gtgggaatca attacctcga aaaagttcag atggcccatc
      721 tgccttctat ttacggtgcc atgctcgccg gcaccgccta cgtgctgatg ggtgcgggca
      781 ttcccctgaa aagtcccggg gtgctcgacc gcttcacctg tcacgagcct gcttcctacc
      841 cgctgcaaat caccggcgcg caggaggcgg acgatacgac catgaccttt gtgccgcggg
      901 aattcatgga gcgcgagatt ccactgctga tccggccgag gttcatcgct atcgtcgcct
      961 ccaacacgct ggcttcgacg atggtgaaga aggctaacgg cagagtggat ggtttcgtta
     1021 tcgaggggcc caccgccggg gggcataatg ccccgcctcg cggccgcctc caactggacg
     1081 aggccggcga accggtctac ggaccgcggg atgccgtgga cctggagaag ctgcgtgagc
     1141 tcggtctgcc gttctggctc gccggcagct acggcagccc ggaaaaattg cgcgcagcct
     1201 ggtcagccgg ggccgcggga gttcaagtgg gaaccccctt cgcattctgc gccgagtctg
     1261 gattgaggaa tgattacaaa caggcgatcc tgaacgatat tgcggccggc aaggcgcacg
     1321 ttttcacgga ttcgctggct tcgcccacga acttcccgtt caaagtggtg caggtgtcag
     1381 gctccctttc cggcagcgag gtctatgcgg cccggccccg catctgcgat gtgggttatc
     1441 tgcgggaggc ctatcgggag ccggacggca cgatccggta ccgttgcgcg gcggaaccgg
     1501 tgacgacgta cgtgtcgaag ggcggcagac tggagaacac gaatggccgg aagtgtctct
     1561 gcaatgcgct gctggcgaat atcggcctcc cgcagatccg cagtggaaag tatgtagaga
     1621 atggtctggt cacagcggga agcagcctcg cggatgtggc ccgattttta ccggagaacg
     1681 gactcgtcta tcaggccggt gatgtaatcg caaagctact gaacgtggaa acgctcccgg
     1741 cccttccgcg gccggaagtc tgcgcgggcg ccgcattgtc agatgccggt cattgttgat
     1801 acgctctatc ccaacgatgg cttgtcgaag gacttcaatc agttggatat gggctcgatg
     1861 ctgcctggtg gccgctgtca ttttgctggc gatgcccgtg tgggctcaaa gacgtgccac
     1921 agggccgctg cgagtgctcc aatcgaatcc acggtggttt acggacggat ccggcaaagc
     1981 ggtctatctg gcggggtcgc acacctggtg gactttgcag gacaatgatc tgctttatcc
     2041 tgcgaagacc aaccctcctc accggtttga ctttgacggc tacctcgatt tcctggagcg
     2101 gcacaaccac aacttcttcc ggctatggcg ctgggaaccg ccgaaatgga ccgacagcta
     2161 cgacaagaaa gtggcgtact gcacgcccca tccgtgggtg cggaccggtc ccggcacagc
     2221 cgctgacggg cagccgaaat tcgacctgac ccgtttcaac caggagtatt tcgaccggat
     2281 gcgagtgcgt ctgaccaaag ccggcgatcg cggaatatac gcggcggtga tgctctttga
     2341 aggttgggcg atgcagttca ccgacgggtg gacttatcat cccttcaatg gagcgaacaa
     2401 catcaacggg gtcgacgccg acatcaactg cgacggtaaa ggcttggagt tcaactcgct
     2461 tgagctcgga gcgatggcgc gccgggtgct cgaccttcag gaagcttacg tacggaaagt
     2521 gatcgacacg gtcaacgacc tggacaatgt gctctatgaa gtttcgaatg aggcattcgc
     2581 cgggtcaacg gattggcagt accacatgat tcgcttcgtg aagcaatacg aatcggataa
     2641 acccaagcag cacccggtgg gcatgacgtt tcaataccgc ggaggcgcca acgctgtgct
     2701 ttacgccagc cctgccgact ggatatcgcc caacgaaggc ggcagcgcgg agagtttccg
     2761 ggataacccc tgtgctgact gcacaaccaa agtggtggtc agcgacacgg atcatctgtg
     2821 gggtcacacc gggggcgaca gcgtatgggt atggaagagt ttctgccgcg ggctgaacgt
     2881 gctttttatg gaagagctgc ttccgtcgcc tacctggcag gattcggcgc gacttgcaat
     2941 gggtcaaacg cggcgttacg ccgggaagat ggacctggca tcgatgaagc ccgaaccggg
     3001 aattgtaagg ggaggctatt gcctggctca ccggggcagc gaatacctgg tgttccagcc
     3061 cggcgtgatc ggacagttca cggtggattt gaaagacgcg ccgggattct tcgatgtgga
     3121 atggctcaat gtaaacaaag atgaaacggt ggccggcaag cccgtgcagg gaggttcagt
     3181 agtcacgttc cgcacgccat tcggaggtcc ggcggcgtta tacctgaagc gctcccaggg
     3241 tgcgagcgcg gcggggaagt aagccccaca ggagtgatat ggaacagaaa ccggttgcga
     3301 tcgataccag cacggttgag tgggaggagc ggttcaacga aaagctgggc aggagcctgt
     3361 ttcgaaaaaa tctgttcgaa gacgccgaga ccggaatgga gattcgcctg gtgcgttatc
     3421 ccgccggcgt agtgaaccca tggcatacgc atccctgcgc ccacggaatg tacgtgctcg
     3481 agggcacgct ggcgaccaac gcgggcacct ttgggccggg gacgtttgtg tggtttcccg
     3541 aaggttccgt catgcaacac ggagcgaccg cggaggcgga tgtgaccgtg ctgttcatca
     3601 ccaataaacc atttcgtatc gattacgttc agcgttgaac cggttcgtac atgtggtcgc
     3661 ggctcatgcg ccgaggccgg ggatgggtgc gttttgcgaa cagcagttgg aacacatcgg
     3721 cgccgccgtc ctcgaaattt gccgccgacc ctgcaagata gatgagccag gttcgatagg
     3781 tctgccggtc taccgcttcg aggcaggcat ttcgatttgc caggaggcgt tcgagccatg
     3841 cgcggcatgt catgccatag tgacaccgca ggttttcggc atccagaagt tcgaacccgg
     3901 ctctttccgc cgtccgaacg gtttgtgcca ggcgcgacaa cccgcctccg ggaaaaacgt
     3961 aacgatcgat aaagagggtc tcggcatcct cgcggacacc ctccggacga ataatgccgt
     4021 gatttagaaa aagccccgag ttgtcgagaa gccggtaaac cgtccggaag tactgttcca
     4081 ggtggatagg ccccacgtgc tcgaacatgc cgacggaggc gatcttgtcg taaggtcccg
     4141 tcagtttgcg atagtcggcc agacgcaccg aagcagagtt gcccaaccgg cgagcttcga
     4201 tgaggcggca ggccagtttg gcctggttct cgcttaaggt gcaacccgta gcaagcacgc
     4261 catagctccc ggcggcgcgc agcagcaagc tgccccaccc gcaacctata tcgaggaagc
     4321 ggtcgccggg ttgcaagtcc agcttgcggc agatgtgatc cagcttttcc aactgcgcgc
     4381 gatccaggct ccattcggaa ttccggaagt atgcgcatga atacaccatg cggctgtcca
     4441 gaaatagtcg ataaaagtcg ttcgagcggt cgtaatgaaa gcggatgtcg cgcgtggttc
     4501 gcatgcgagt tgcaagcctg gttcgcaagt gccgcaggta caggagcagc gtttcgaacc
     4561 agggccggcc gccgctgccg gtgcttgcgc gcttgaagcg gacggcagcg accatgtcgc
     4621 ctcgaatatc gaactccccg cggacaaagg cattcgccgc ggacatcata ccggtttcga
     4681 ggagccactg aaactgcttt cgattctgga gtactaacgt gaagttgggg gcgccagaga
     4741 cgataggagg agtaccgtcg caaaattcgg ccgcaaacgc cgtagtatcg ttccttgttt
     4801 gcgttcgccc tcgagcggcc ggcgcaacag tgtggaacat gtcgttcgcc tcagctcaaa
     4861 gatcaactgc caacacttta attttagctc ccatgccggc gggcgattcg atatcttaca
     4921 aggatgcgga aaaggagacg acctccggcg gggatgcaac tttttttgtt tcgcgtatcc
     4981 gtccttctgg cgatggcggc cgcagcgggg ttttcacaaa gcggtgatgt tctttacaac
     5041 ggtatccgcc ttccggccca gtggcctcca acgggaatca aactcagtca cgaaccactg
     5101 cccgatccac cttacctggc atcgcctccg gacattattc cgatcgatgt ggggcggcag
     5161 ttgtttgtgg atcatttcct gattgaggaa acgacgctgc gccgtacctt tcaccaggct
     5221 gaatacttcg caggcaatcc cgtgctgaaa ccagacaagc catgggagct gagacgcgat
     5281 gggggtggag gggcaatgcc cttcagcgat ggcgttttct acgatccgca tgaccgtctt
     5341 tacaagatgt ggtacctggg tctgggtgcg acgttgtacg cgacatcgaa agacggtatt
     5401 cactgggaca aacccgaact cgatgtagag cctggaacga atgtcgttca tcgcgggaat
     5461 cgcgattcca gcaccgtatg gctcgacctg gaagagagcg atccgcaacg gcgatacaag
     5521 tttctctaca acccaggcaa catgcgtccg atgatactgc acttttcttc ggacggaatt
     5581 cactgggggc agcccgccgc gcagagtgtg aaagtggcgg atcgcacgac actcttccgg
     5641 aatccattcc gcaaggtatg ggcctacagt atccgcgacc atacgcccgg agtgatcagc
     5701 ccacagcgta tgcgcagacc cggcgaacat gtacgattcc gcacttatgc ggaatccccg
     5761 gatatcgccg cggggttaga gtggaagccc gtaccctggg ttggggcgga ccggctggat
     5821 gccgcgcgag tggacctcaa cgcgaggccg gagctgtaca acctggacgc ggtcgcttat
     5881 gagagcgcta tggtgggcct gttcagtgtc tggcggggac aattcaaaga ccgggagaag
     5941 ccgaacgaga tcgtggccgg attcagccgc gacggttttc actggtaccg cccgtcacgc
     6001 cgcgcgttca ttcccgtctc cgcgcgccac ggcgactgga atcacggcaa cgtacagtcg
     6061 gcgggcggag tctgcctgtt ggtgggggat cgtctcttct tttatgtgag tggccgctcg
     6121 cgggctcccg gtgtcatagg gcagaacagt acggggctgg cgacgctacg gcgcgatgga
     6181 tttgtttcca tggatgccgg tgccgtcgaa gggacactga ccacgcgtcc ggtttccttt
     6241 cgcggcacgc ggctgttcgt gaacacgaac acggaagcgg gtgaattgac ggtagaggtg
     6301 ctggaccaga aaggccgcgc gatcccggcc ttttcgcgcg cacagtgcat tcccgtgcgc
     6361 gccgataata cagcgcgcga ggtgagctgg aagaatgcgg atctgtcgtc tctttccgga
     6421 aagcccgttc gcttccgttt tcacctgagg cacggcaagc tctattcctt ctgggtaagt
     6481 cccgacgctt ctggcgccag ttacggatac gtgggtgcgg gcggaccagg attcacaggc
     6541 ccaacggata ccgtgggaac cggaatttat cagcattgct gcgcgagcga gatcctgcac
     6601 taaccggcat acggcaaggg ggggcagcgt gaaacgacgg aagctgttcg ggggctctat
     6661 cacgtgtgga gttttattgt gggctgtgca ttcgacactg ctttgcgcac aggaatccgg
     6721 ccgcggacag agcttgtaca acagtctctg catgagttgt cacggcggcg atgggaacgg
     6781 cggagagcgg gctcccggca ttgtgcggca ggtgcgtgcc cgggatgacc gccaactggc
     6841 ggaattggtc cggcagggga ttccgagcgg tggaatgccc gcgtacaagc tggccgagga
     6901 gccgatgcgt gagctgatcg tgtttctgcg gtcgttgcgc ccggcgcgca cgcggccggc
     6961 ccgccgggca aaggtcgaaa ccatcgcagg gagggccctc gaaggagtcg tgctgaatca
     7021 gagcgccagt gagatgcaga tgcgaagcga cgacgggcgg ctgcgtcttt tgcggaggac
     7081 tggcgatcga taccgcgaag tgacttcgca aacagactgg ctgacttaca acggcgctga
     7141 tggcggaaac cggtacagta cgctcgcgca gatcacgaag aaaaacgttt cgcgcctggc
     7201 tccggcctgg gtctttcctt tatcgaacgc ggcgcggctg gaggtaacgc cggtggtgat
     7261 gcaaggcgtt atgtacgtca cggcggcaaa tgaatgttat gccctcgatg ccggctccgg
     7321 gcgcgaattg tggcattaca ggcggccgcg tacgccaggc gtggcggggg acgcaggggg
     7381 tggcatcaat cgcggtgtgg ccgtctccgg cgagcgcgta ttcatggtga cggaccacgc
     7441 ccatctcatc gcgctcaacc ggttcacggg agcgcttctc tgggacacaa cgatggccga
     7501 ctacaaggag aattatggtg ccacatcagc gccgctggtt accggcggtc tggtggtgtc
     7561 gggccattcg ggaggtgatg aaggtgtgcg cggcttcctc gcagctttcg atcaagccac
     7621 ggggaaggag gtgtggcgct tctggacggt gccgaaaccg ggag
//
LOCUS       DASTMK010000466         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_408050, whole genome shotgun sequence.
ACCESSION   DASTMK010000466 DASTMK010000000
VERSION     DASTMK010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408050"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VFQ79_19620"
     CDS             <1..1170
                     /locus_tag="VFQ79_19620"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEU0141941.1"
                     /translation="RRLAAAGYDGALCGKLHLSSPFGREEPRIDDGYRIYDWSHQPHP
                     EAGWPDSVHAYNRWLHEHGVTWEGLYRKRKIAGYPDEYNAGMPAEYHELAWEAERTLH
                     YIEGGLKGPWFASINIFAPHNPFDPAPEYLAKMKPESLPMPLYRDGEEREQARFASAV
                     AHQTKTPVPPASYPAQHMKACYYAMIEHIDWYVGRMVEALERTGQRENTLIVFMSDHG
                     EMMGDHCLRLKGCRFFEGAVRVPLILSWPGGGLRNGMRNDALVELTDIAPTLLEAAGI
                     ADPGDMDGKSLLGLARGTTNAKAHREFAWSEYHDSQTNALRSHASMIRDRSHKLTLYH
                     GTGLGELFDLDADPQEFENLFEKPEHARLRQRLTDQLVDHLALRTDLGSPRIGKY"
     gene            complement(1194..2774)
                     /locus_tag="VFQ79_19625"
     CDS             complement(1194..2774)
                     /locus_tag="VFQ79_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0141942.1"
                     /translation="MKWLSLFLVLSLAVASAIAADSARVQAMKADLAGEVDAMSKQAQ
                     VMTDMVFSFGELGFQEIETSKYLTGILEKEGFTSERGIAGIPTAWVAKWGSGKPVIAL
                     GSDIDCIPQASQKPGVAFHDPILAGAPGHGEGHNSGQPLNIVAALAVKKIMEREKIPG
                     TLLLWPGVAEEQLGTKAYYVRDGLFKDVDAVIFTHVADNFGVSSGAGAGSGMISVEYH
                     FQGESAHSAMAPWRGRSALDGVELMNIGMNFRREHLPLAQRTHYVISDGGDQPNVVPQ
                     NATVWYYFRQLTYDSIMNLWKIGDRMAEGATLMTDTTVSSRVLGSAWPGHFNKPIAEA
                     VRTNIEAVGLPAWSNDDQTLAKALQKELGVEQEGLPVKLNERANRDQEPDPRARLGGG
                     SDDIGDVSWNVPTVVLRFPSNIPGLPGHNWANAIAMATPIAHKGVVAGAKVNAMTILD
                     LLTNPELITSAWDYFRNVQTKNETYKPLIRPGDKPAIWLNEKIMQEYRPRMKEFYYNP
                     EKYDTYLEQLGIEYPTVR"
BASE COUNT          541 a          824 c          933 g          586 t
ORIGIN      
        1 cgacggctgg cagccgcggg ctatgacggc gcgttgtgcg gcaagctgca tctctcaagc
       61 ccgttcggac gggaggagcc ccggattgac gacggctatc ggatctacga ttggagccat
      121 cagccgcatc cggaagcggg ctggccagac agtgtgcatg cctacaaccg ctggctccac
      181 gagcatgggg tgacctggga gggtctctac cgaaagcgga agatcgcggg ctacccggat
      241 gagtacaacg cggggatgcc ggccgagtat cacgaactgg cctgggaagc cgagcgaacc
      301 ctgcattaca tcgaaggcgg gttgaagggc ccatggttcg ccagcatcaa catcttcgca
      361 ccgcataacc cgttcgatcc ggcgccggaa tacctcgcga agatgaagcc tgagtcgctt
      421 cccatgccgc tttatcgcga cggggaggaa cgggagcagg cgcgattcgc cagcgcggtt
      481 gcccaccaga cgaagacgcc cgtgcccccg gcgagctatc ccgcgcagca catgaaggcc
      541 tgctactacg cgatgatcga gcacattgac tggtatgtgg ggcggatggt ggaggcgctc
      601 gaacgtaccg gccaacgcga gaacacgctg attgtcttta tgagcgacca cggcgagatg
      661 atgggcgacc actgccttcg tctgaagggt tgccgcttct tcgaaggcgc agtgcgggtg
      721 ccgctgattc tttcgtggcc cggaggcggg ctgcggaatg gaatgcgaaa tgacgcgctg
      781 gtggaactca ccgatatcgc acccacgctc ctcgaggctg ccgggattgc ggaccccggc
      841 gacatggacg gaaagagcct gctggggctg gcgcgcggga cgacgaatgc gaaggcgcat
      901 cgggaatttg cgtggagcga gtatcacgat tcacagacga atgccctgcg atcccacgca
      961 tcgatgatcc gcgaccgcag ccacaagttg acgctttacc atggcaccgg tctcggagaa
     1021 ctcttcgatc tggatgccga cccgcaggaa ttcgaaaatc tcttcgagaa gccggagcac
     1081 gcccgattgc gccagcgatt gactgaccag ttggtggacc atcttgcgtt acgcaccgat
     1141 ctcggaagtc cgcggattgg caaatactaa cgcaacgccc tcgtgttcgc agctcacctt
     1201 accgtggggt actcgatgcc gagttgctcc aggtaggtgt cgtatttctc cgggttgtag
     1261 tagaactcct tcatgcgagg gcggtattcc tgcatgatct tctcgttcag ccagattgcg
     1321 ggcttgtcgc cggggcggat caggggcttg taggtctcgt ttttcgtttg gacattcctg
     1381 aaatagtccc acgcggaggt gatcagttcc ggattggtga ggaggtcgag gatcgtcatt
     1441 gcgtttacct tggctccggc taccacgcct ttgtgcgcga tgggggtggc catggcgatc
     1501 gcatttgccc agttgtggcc ggggagcccg gggatgtttg aggggaatct gagaacgacc
     1561 gtggggacgt tccaggagac gtcgccgatg tcgtctgagc cgccgccgag gcgtgcacgg
     1621 ggatcgggtt cctgatcgcg gttggctcgc tcgttcagct tgacggggag gccttcctgt
     1681 tccacgccaa gttccttctg gagcgctttg gcaagggtct ggtcatcgtt gctccatgca
     1741 ggcaagccta cggcctcaat gttcgtcctc acggcttcgg cgatgggctt gttgaaatgc
     1801 cctggccagg ccgagccgag caccctcgaa cttaccgtgg tatcggtcat cagggttgca
     1861 ccttcggcca tgcggtcgcc gatcttccag agattcatga tggaatcgta ggtaagttgg
     1921 cggaagtaat accagacggt ggcgttctgc ggaacgacgt tcggttgatc tccgccgtct
     1981 gagatgacgt agtgggtccg ctgggcgagg ggcaaatgtt cgcggcggaa gttcattccg
     2041 atgttcatca gctccacgcc atcgagcgcg cttctgcctc gccagggagc cattgccgag
     2101 tgagcgcttt cgccttgaaa gtggtattcg acggagatca ttccactgcc tgcgccggcg
     2161 ccgctggaga cgccgaagtt gtcggccacg tgcgtgaaga tgacggcatc cacgtcttta
     2221 aagaggccgt cgcggacata gtaggccttt gtgccgagct gctcttcggc aacgcccggc
     2281 cagagcagaa gggtgccggg aatcttctcg cgctccatga tctttttcac cgcgagggcg
     2341 gcgacgatgt tgaggggctg gcctgagtta tggccttcgc cgtgtccggg cgcacccgca
     2401 aggatggggt cgtggaaggc gacgccaggc ttctgggagg cctgcgggat acagtcgata
     2461 tccgagccaa gggcgatcac tggcttgccg ctgccccact ttgccaccca cgcggtgggg
     2521 atacctgcga tccctcgctc actggtgaag ccttccttct caagaatgcc ggtaaggtat
     2581 ttcgacgttt cgatttcctg gaatcccagc tctccgaagc tgaagaccat atcggtcatc
     2641 acctgcgcct gcttggacat ggcatccact tccccggcca ggtcggcctt catggcctgc
     2701 accctggcag aatccgcggc aatggcagag gcaaccgcca gggagaggac gaggaagaga
     2761 gacagccatt tcattcgaat actcctgctt cggcgcgatg ttgggcagaa tggcgcggag
     2821 ggggtcacga gacaatccgc gcgcaagaat ccacagaatg ctggcgatta tttcacagcc
     2881 tgtt
//
LOCUS       DASTMK010000467         4682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_25479, whole genome shotgun sequence.
ACCESSION   DASTMK010000467 DASTMK010000000
VERSION     DASTMK010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25479"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFQ79_19630"
     CDS             <1..248
                     /locus_tag="VFQ79_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141943.1"
                     /translation="DAGSEGALADRILTGQTDQNGTHIWERNIPPGTYRPVAARTEVD
                     YSPEGVHALWQARSQAPEITVGPNGSADVKVVIQKER"
     gene            complement(312..1409)
                     /locus_tag="VFQ79_19635"
     CDS             complement(312..1409)
                     /locus_tag="VFQ79_19635"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU0141944.1"
                     /translation="MSNGLQLLITLAGVTLLFGCAQANGTMEGGARQALRLCNGRVAL
                     LPPAYFLLAALLAMVGPGAILSTALLAPFAMSTGYRAGVPPFLIALMVANGANAGNMS
                     PFSTVGAIANTLMTQEGLGGHEIRVWFFHAAAHVVVAIAAYLLFGGLALFREGSSETV
                     PAPPRMQRKQAVTLGITALWIAGVALLHWPLGWSAFGSAGLILLLRLASIREAVVHMP
                     WKIIALVVGVSTVVGLIDEAGGFAWFQELFARLATSDSVYAVIAFITGAISAYSSTSG
                     VVLPAFLPLAPGLAAHLPGVDPLALSISIVIGSALVDVSPLSTLGALCVAAAPEGSHR
                     TALFRNLMIWGFSMTVVGAAICYFWAPFFSV"
     gene            complement(1406..2869)
                     /locus_tag="VFQ79_19640"
     CDS             complement(1406..2869)
                     /locus_tag="VFQ79_19640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0141945.1"
                     /translation="MTLPELFELSFTLHRDEAALEWDRTYTFGEIDARASQMAHALAA
                     RGIATGDRLCVYLSNSITYIDLFLAATRLGVIFVPVNILYRDREIRHILEDAEPVAIV
                     SDEAGRVHIPGGAPIWPIEDLAREASAAPVTRSVAVTDGDAPAALVYTSGTTGVSKGA
                     IVTHNAFAVNALNLISCWQINSADRLLLTLPLFHVHGLGNGLHCWLISGCRMRLTERF
                     DHAHAVEWMLDFEPTLFFGVPTMYVRLLNAEEDAARRIGERMRLFVSGSAPLPSQVLE
                     AFRNRFGHTILERYGMSETLMNISNPYVGERRPGSIGIPLPGVSVRNLDPEGNPVKDG
                     ETGELYVRGSNVFAGYWRRPEATAAAFRDGWFRTGDLAVRAADGYYTLQGRAGDLILS
                     GGFNIYPREIEEFLAEQSGVAEVAVAGVPDPLRGEVPVAWVVVRGEADCGALESACRQ
                     HLASFKIPRAFVIVDKLPRTALGKVQKHLLPPWDQVR"
     gene            complement(3167..3937)
                     /locus_tag="VFQ79_19645"
     CDS             complement(3167..3937)
                     /locus_tag="VFQ79_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0141946.1"
                     /translation="MAAISTLIVDDEELAREELSYLLREFRDVEILGFASNGVEALKL
                     IEDFEPDLVFLDVQMPGLDGLNVIRRLREKEIPAPAFILTTAYEQYAVEAFREEAFDY
                     LLKPIEKERLDVTMERARRQLAEKAKLHPADPTEVTPRAAGQRNAKVMVKAHSRNFIV
                     DEQEIVFATIDDGLITVVCAHVEGTCNFRTIEELQSSLDPDLFWRVHRSYLVNINRIR
                     EVIPWFKSSYQLRMDDKAQTVVPVSRVQTKRLRSLLKL"
     gene            3954..>4682
                     /locus_tag="VFQ79_19650"
     CDS             3954..>4682
                     /locus_tag="VFQ79_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU0141947.1"
                     /translation="MSGDAAPEHNGKLRDWPPLIPLASRLRRGPLKSSQTPSIAPAEG
                     KLGVLIPGMGAVTTTFIAGVAAIRKGLGNPIGSLTQMGTIRLGKRTDNRTPKINEFVP
                     LASLDDLVFGGWDIFPENMYEAAVNADVLTPQSLGPLKDELSAIQPMSAVFDKNYVKL
                     IDGPNVKQGATLMDKAEMLMDDIRQFKERNNCTRLVMIWCGSTEVFHSKIHVHETMEA
                     FEAGLRNNDPAIAPSQVYAYAALKL"
BASE COUNT         1032 a         1415 c         1390 g          845 t
ORIGIN      
        1 tggacgccgg cagcgaaggc gccctcgcgg accggattct cacgggccag accgatcaga
       61 acggcaccca catctgggag cgcaacatcc cgcctggcac gtatcgcccg gtggccgcgc
      121 gaacagaagt ggattactca cccgaaggcg tccatgcttt gtggcaggcc cgttcgcagg
      181 ccccggaaat cacggttggc cccaacggca gcgctgacgt gaaggtagtg attcagaagg
      241 aacgatgaga gatcggccga agtttgtaag gaccagatcc ccgaattgac aaacggaata
      301 gacccgagct gttaaacgga aaagaaaggc gcccagaagt aacagattgc agcgcccacg
      361 acggtcatgg agaagcccca gatcatcagg ttgcggaaga gtgcagttcg gtgagagcct
      421 tcaggcgcgg ctgcgacgca aagggcgccg agcgtcgaca ggggagatac atccaccagc
      481 gcggaaccga taacgatgct gatcgacaac gctagcggat cgacgccggg gaggtgcgcc
      541 gcgagtcccg gcgctaaggg caggaaggcg ggcaaaacga ctcccgaagt gctggaatat
      601 gccgagatgg cacccgtaat aaaggcgatc acggcatata ccgaatccga ggtagccagc
      661 cgcgcgaaca gctcctggaa ccacgcgaag cctccagctt catcgataag gccgacaacg
      721 gtgcttaccc cgaccacgag agcgataatc ttccatggca tgtggacgac ggcctcccgt
      781 atggacgcga gccggagcag caggatgagc cccgccgatc cgaaggcgga ccacccgagc
      841 ggccagtgaa gcaaagcgac gccagcgatc cagagagcgg tgatgccaag cgtaaccgct
      901 tgcttacgct gcatgcgagg cggagccggg accgtttccg aggaaccttc ccggaatagc
      961 gccaatccac cgaacaggag atatgcggca atcgccacga cgacgtgagc cgcggcgtga
     1021 aaaaaccaca cccgaatctc gtgtccgccg agcccctcct gcgtcatgag cgtgttggcg
     1081 atggcgccca cggtggaaaa gggcgacata ttccccgcgt tggcgccatt cgccaccatg
     1141 agcgcaatca gaaatggcgg taccccggcg cgataaccgg tgctcattgc gaaaggcgcg
     1201 agcagcgcgg tgctcaggat ggcccccggc cccaccatgg cgagcagcgc ggcaagcaga
     1261 aagtacgccg gaggcagcag cgccactcgt ccattacaca gtcgcagagc ctggcgcgcc
     1321 cccccttcca tcgttccgtt cgcttgagcg cagccgaaca ggagagtcac cccggcaagg
     1381 gttatcagca gttgcaggcc gttgctcacc gcacctgatc ccatggcggc aggagatgtt
     1441 tctggacttt cccaagagcg gtgcgcggta acttatcgac gatgacaaac gcacgcggaa
     1501 tcttgaaaga cgccaggtgc tggcggcacg cggattcgag agcgccacaa tccgcttcgc
     1561 cgcgcaccac cacccaggcc acgggaacct ccccgcgcag ggggtcggga actccggcca
     1621 cggctacttc cgccacgccg ctttgttccg caagaaattc ctcgatctcc cgcggataaa
     1681 tattgaatcc accggacagg atcaaatcgc ctgcgcggcc ctgcagtgtg taatatccgt
     1741 cggcggcacg gacagcaaga tcccctgtcc ggaaccatcc atcgcgaaat gcggcggcgg
     1801 tcgcctccgg gcgtcgccaa tatccggcga atacgttcga ccctcgtacg tacaactctc
     1861 cagtctcccc atccttcaca gggttccctt cagggtcgag attgcgcacc gaaacgcccg
     1921 gcaggggaat gccgatgcta cccggacgcc gctcgcccac gtagggattg gagatgttca
     1981 tgagcgtctc gctcatgccg tagcgctcga ggatcgtgtg tccgaatcgg ttgcggaacg
     2041 cttcgagcac ctgggacggc aatggcgcgg agccggaaac gaagagccgc atccgctcgc
     2101 cgattcgccg ggcggcatcc tcctccgcat tcagcaggcg tacatacatc gtcggcacgc
     2161 cgaagaaaag cgttggctcg aaatccagca tccattccac ggcatgggcg tggtcaaacc
     2221 gttcggtcag ccgcatgcga caaccgctga tcagccagca atgcaggcca ttgccgagcc
     2281 cgtggacatg gaagaggggc agggtgagca gcagacggtc ggcggagttg atctgccagc
     2341 aggagatcag attcagcgca ttcaccgcga acgcattgtg cgtgacgatc gcccccttgg
     2401 aaacgccggt cgttccggac gtgtaaacaa gggcggcggg cgcgtcgcca tccgtcacgg
     2461 ctacgctccg ggttaccggc gccgcggagg cttcccgggc gaggtcttcg atcggccaga
     2521 taggtgcgcc gccgggaata tgcacccggc ccgcctcatc gctgacgatg gccaccggtt
     2581 cggcatcctc cagtatgtgg cggatctccc gatcgcgata caggatgttg accggaacaa
     2641 aaatgacacc gagacgcgtt gcggcaagaa atagatcgat ataagtgatc gagttcgaga
     2701 gatacacgca tagcctgtcg ccagtcgcga ttcctcgcgc ggcgagcgcg tgcgccatct
     2761 ggctagcccg cgcgtcaatc tcgccgaagg tgtacgtgcg gtcccattcg agcgcggcct
     2821 catcgcggtg aagcgtaaac gaaagttcga aaagttcagg cagagtcaag cgtcgccagt
     2881 ctagcacaac gtccaagcag gaagctgccc gcagaattgt ccaacgcgga atcagcctga
     2941 aggtgggagt gtttctaaca caaagtgagc ctgaagcgaa ggtagggaga ttgctttggg
     3001 ctgggatgct acggatgaaa ggagattcca gacctcagca tccgggtctt caggctcatt
     3061 cttcgattgg gaaatgctat taggccttgg cgtaaactcc aaggccaaga ccgcttgcga
     3121 cgtctccggt catgcgcttc gcctgctatc tcgcgaaccc gaattgctaa agcttcaaca
     3181 ggcttcgcag acgtttcgtc tgtacgcggc ttaccggcac tacggtctgc gccttgtcgt
     3241 ccatccgaag ctggtagctc gacttgaacc agggaatcac ttcgcggatg cggttgatat
     3301 tcaccagata agagcgatgg acccgccaga aaaggtcggg gtcgaggctc gattgcagtt
     3361 cttcgatggt gcgaaagttg caagtgcctt cgacgtgggc gcatacgacg gtgatgaggc
     3421 cgtcgtcgat ggtggcgaag acgatctcct gctcgtccac aatgaagttc cggctgtgcg
     3481 ccttcaccat caccttggcg ttccgctggc ccgctgcgcg cggagtcact tccgtgggat
     3541 ccgcgggatg caacttcgct ttttcggcga gctgacgtcg agcgcgctcc atggtcacat
     3601 ccagccgttc tttctcaatc ggcttcaata gatagtcgaa cgcttcctcg cggaaggctt
     3661 ccaccgcgta ttgttcgtag gctgtagtga ggatgaaggc gggggcggga atctccttct
     3721 cccgcagccg tcgaatgacg tttaacccgt ctaacccggg catctgcacg tccagaaata
     3781 ccaggtccgg ttcaaagtct tcaattagtt ttaatgcttc cacaccgtta ctggcaaacc
     3841 caagaatttc tacatcacga aactctctca gcaggtagga tagttcttca cgcgccaact
     3901 cctcgtcgtc gacgatcaac gttgagattg cagccacgtt tcccccttcc ggcgtgagcg
     3961 gtgatgccgc ccctgagcac aatggtaaac tacgtgattg gcctccgctg attcctcttg
     4021 cgtcgaggct taggagaggt ccattgaaaa gtagtcaaac cccgtcgatc gcgccagcag
     4081 aaggcaaact gggcgtttta atcccaggaa tgggtgcggt caccacgacg tttattgccg
     4141 gcgttgccgc catccggaaa ggacttggga atcccatcgg gtcactcacc cagatgggca
     4201 ccattcgcct cggcaaacgt actgataatc gcactccgaa gatcaacgaa ttcgtgcccc
     4261 tcgcgagtct cgacgatttg gttttcggcg gctgggatat cttcccggag aatatgtacg
     4321 aagcggcggt gaatgccgac gtgttaacgc cccagtccct ggggccgctc aaggacgaac
     4381 tctcggccat tcagccgatg agcgccgtct tcgacaagaa ttacgtgaag ctgattgacg
     4441 gccccaacgt caagcagggc gccaccctga tggataaggc cgaaatgctg atggacgata
     4501 tccgtcagtt caaagagcgc aataactgta cccgcctggt gatgatctgg tgcggttcca
     4561 ccgaagtgtt tcattcgaag atccacgtcc acgagacgat ggaggccttt gaagcgggcc
     4621 ttcgcaataa cgatccagcg attgccccca gccaggttta tgcctatgcc gccctcaagc
     4681 tg
//
LOCUS       DASTMK010000468         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_120129, whole genome shotgun sequence.
ACCESSION   DASTMK010000468 DASTMK010000000
VERSION     DASTMK010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120129"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFQ79_19655"
     CDS             <1..293
                     /locus_tag="VFQ79_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU0141948.1"
                     /translation="DAKDVIYATIDDGLITIVTTAIEGQSNYRTIEELQSNLDPDLFW
                     RVHRSYLVNINRIKEVIPWFKSSYQLRMDDKKQTEIPVSRVQTKRLRALLKL"
     gene            complement(351..1574)
                     /locus_tag="VFQ79_19660"
     CDS             complement(351..1574)
                     /locus_tag="VFQ79_19660"
                     /inference="COORDINATES: protein motif:HMM:NF024303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HEU0141949.1"
                     /translation="MKPLCEGPGAAGQILSEQYARAVQTLRNCAHPAGLNASACGSGH
                     QQVWARDSMISLLGGALVADRTVQSALEASMETLERHQAPSGCIPSNVDPVTGRPNFR
                     AYADSGLWFVIGSSILSPRLDAVRRVLRWYECQDVDQSGLISMQEASDWQDLFCTRGK
                     GLYVNCLYVLALQKAAAMAHSAGDEAVAAARLEQSRKVSQKIHRLFWYAPDGGMRRHI
                     EHSFSTDNIEHDSLGRRRWIPTRRVLPDEQYYLPYLGFREVGEWFDSLGNLLAILSGV
                     AGEDETARILDLMDRYNIAGEPVKAIYPAVQPGEPDWRDYYGSLNLPHHYHNGGIWPF
                     IGGFYVAALVKAGRLLQAEAALERLAHLNAAGDFNEWHHGQTVQPMGVAGQAWSAGMY
                     LFACECVRRRSVPLL"
     gene            complement(1571..2446)
                     /locus_tag="VFQ79_19665"
     CDS             complement(1571..2446)
                     /locus_tag="VFQ79_19665"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEU0141950.1"
                     /translation="MFAIGERLRGERLKRGLSIHEVAEKTKISSRYLEAIEAGEPGDL
                     PGEFFYRSFVRQYANLLNIEVGELEPELETPAPAFAGYGERSYDPRHDHLPVDPLPSP
                     SGGSDSFNRLPLSIALLVAVLIGCAGLYSLWLKGWRSPEEPSATVATSNSNASQPEAS
                     APARPASSASAGAAAPVPAPDGKLAVDLSATEPVWVSVSSTGQDLFIGTLEAGGTKSV
                     AGAGPVKIVVGNAGGIEVKINGRSLGPIGPRGQVRIINATADSHQITPPGGAKPAEPS
                     PSPAPANAASANTAT"
     gene            complement(2521..3726)
                     /locus_tag="VFQ79_19670"
     CDS             complement(2521..3726)
                     /locus_tag="VFQ79_19670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0141951.1"
                     /translation="MRICLSLLTGVLALYSVAAAQLAVVGGTVHTSAGAPIRNGVVLV
                     RNGKIESVGPASSVRIPAGYRTVKAAVVTPGLIDAHSVVGLGGYLNQPHDQDQREASA
                     PIQPDLRAMDAYNARERLVEWVRGFGVTTVHTGHGPGALISGQTMIVKTRAEPVARSV
                     LVPEAMLAVTIGDSARVQQGKSPGTRAKMLAMLRSELLKAQDYARKRQKDDSSQARDL
                     RLDVLSQVLNRELPLLVTAQRARDILSALRLVEEFNIRVVLDGAAEAPLVMGEIKRAG
                     VPVIVHPTMYRSSGETENLSFETAAKLHQAGIPFALQSGFEAYVPKTRVVLFEAAVAA
                     ANGLGPGAALDSITIGAARLLGIDKRAGSLEPGKDADIALYDGDPFEYTTHCTGVMID
                     GELVSEESR"
     gene            complement(3723..>4116)
                     /locus_tag="VFQ79_19675"
     CDS             complement(3723..>4116)
                     /locus_tag="VFQ79_19675"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0141952.1"
                     /translation="TGGALEKAGALVGFHTDDGITDSRLFLRSPALAVRAGMSRGKAL
                     FGVTMANAMMLGLEDRVGSLEAGKDADLVILSGDPLSVYTKVLETWVDGTKVFDRADP
                     KDRLYAVGGFGASHDQGSEIFYVEEFEQ"
BASE COUNT          825 a         1293 c         1219 g          779 t
ORIGIN      
        1 tcgatgcgaa ggacgtcatc tatgcaacca tcgatgacgg actgattaca atcgtcacca
       61 ccgccatcga aggacagtcg aattaccgca cgattgagga acttcaatcg aacctggacc
      121 cggatctttt ctggcgggtg caccggtctt acctggtgaa tatcaaccgg atcaaagaag
      181 tgattccgtg gttcaagtcg agctaccagc tccggatgga tgacaagaag cagaccgaga
      241 ttccggtcag ccgcgttcaa acgaagcgtt tgcgcgccct cctgaagctg taacggcatg
      301 cgcggtcgcg gcttggaacg gagccgcgcg cgcaagcaag cgggtttcac ctacagcagt
      361 ggaacactcc ggcgtctgac gcactcgcat gcaaagagat acatacccgc agaccatgcc
      421 tgcccggcta cacccatcgg ctggacagtc tggccgtgat gccactcgtt gaagtcaccc
      481 gcggcgttga ggtgtgccag ccgttcgagc gcggcttccg cttgcaggag acgtcctgcc
      541 ttcaccagcg cggcgacgta gaagcctcca atgaacggcc agatgccgcc attgtggtaa
      601 tggtgcggaa gattcaatga gccatagtag tcccgccaat cgggctctcc cggctgaacg
      661 gcggggtaaa ttgctttgac aggctccccg gcaatgttgt accggtccat cagatcgaga
      721 attcgcgccg tttcatcctc gccggccaca ccggagagga tcgccagcag atttcccagc
      781 gaatcgaacc actcgcccac ttcgcgaaaa cccagataag gcaggtaata ctgttcatcg
      841 ggcagaacgc ggcgagtggg aatccaccgg cggcggccga gcgagtcgtg ctcgatgtta
      901 tcggtgctga aggaatgctc gatgtgacgc cgcatgcctc cgtcgggcgc ataccagaag
      961 agccggtgaa tcttttgaga caccttccgg gactgctcca gccgggcggc cgcaaccgct
     1021 tcatcgccgg ccgagtgggc catggcggct gctttttgaa gtgccagaac atagagacag
     1081 ttcacgtaca gacccttgcc ccgcgtgcaa aacaggtcct gccagtccga agcctcctgc
     1141 atgctgatga gcccgctctg atcgacatcc tggcattcgt accagcgaag cacgcggcga
     1201 accgcgtcca accggggcga gagaatgctg ctgccgataa cgaaccagag accactatcg
     1261 gcgtaagcgc gaaaattggg ccgcccggtg acagggtcaa cgttgctggg gatgcatccc
     1321 gaaggtgcct ggtggcgctc cagggtctcc atactggcct ctagcgcgga ctgcacggtg
     1381 cgatcggcta ccagcgcacc gccgagaaga gagatcatgc tgtcgcgcgc ccaaacctgc
     1441 tggtgcccgg agccgcaggc cgaagcgttc aaccccgcgg ggtgcgcgca gttacgtagc
     1501 gtctgaaccg cccgtgcata ttgttccgat aggatctgcc ccgccgcgcc gggtccttcg
     1561 cacaatggtt tcaagtggct gtattcgcgc tggccgcgtt cgccggtgcc ggtgaagggg
     1621 acggctcagc gggtttcgct cccccgggag gtgtaatctg gtgcgaatcc gccgtggcgt
     1681 tgatgatccg cacctggccg cgcggtccaa taggccctag agaccttccg ttgatcttca
     1741 cttcaattcc gcctgcattg cccaccacga ttttcaccgg cccggcgccg gcgacggatt
     1801 tggtaccgcc cgcctccagc gttccaatga agaggtcctg gcccgttgag ctgacagaaa
     1861 cccataccgg ctcggtggca gacaaatcca cagccagctt tccatccgga gccggaaccg
     1921 gcgccgcggc gcccgcacta gcggaggatg ccgggcgggc cggcgcagag gcttcgggtt
     1981 ggctcgcgtt cgagttggat gtggcaaccg tcgcggaggg ctcttccgga cttcgccagc
     2041 ctttcagcca tagcgaatac aggccggcgc agccgataag cacggccacc aggagcgcga
     2101 tcgacaacgg cagcctgttg aacgaatcgg aacctccgga tggtgaaggc aagggatcta
     2161 ccggtaaatg atcgtgccgg ggatcgtaac tccgctcgcc atagccggcg aatgcgggag
     2221 cgggcgtttc caattccggc tcgagttcgc ccacctcgat attcagcaaa ttcgcatact
     2281 gacggacaaa gctgcgataa aaaaactcgc cgggcagatc tccaggctca cccgcctcga
     2341 tggcctcgag ataacggctg ctgattttcg ttttttcagc gacttcgtgt atggaaagac
     2401 cccgtttaag ccgctcccca cgaagccgtt caccaatcgc aaacattgta gaacggtagc
     2461 tgcccttcat ttaattgtta tcacaaacag ggtttcgtcg cgtctgtttc gtggtgtaat
     2521 tcagcggctt tcttcgctaa ccaattcacc gtcgatcatc acgccggtgc aatgcgttgt
     2581 gtattcgaat ggatcgccgt cgtacaacgc gatgtcggca tctttgcccg gctcaagtga
     2641 accggctcgc ttgtcaatgc cgaggagccg cgccgcgccg atggtaatgc tgtccagggc
     2701 tgctccgggc ccaagaccat tcgccgcggc aaccgcggct tcaaaaagca caacgcgagt
     2761 cttgggaaca tacgcctcga atccgctctg caaagcgaag ggaatgccgg cctggtgcag
     2821 cttggcagca gtctcgaagc tgagattttc ggtctctccg gaggaccggt acatggtcgg
     2881 atgaacgatc accggtaccc cagcccgttt gatttcaccc attaccaggg gcgcttcggc
     2941 ggcgccatcc aaaaccacgc gaatattgaa ttcttccacc agccgcaggg cactgagaat
     3001 atcccgggca cgctgcgcgg tgacgagaag cggcaactcg cggttcaaca cctgggagag
     3061 cacgtccagg cggaggtctc tcgcctggct gctgtcgtcc ttctggcgct tgcgggcata
     3121 gtcctgggcc ttcagcagct ccgatcgcaa catcgcgagc attttggcgc gagtgcccgg
     3181 cgatttgccc tgctgcacgc gcgcggaatc gccgatggta acagccaaca tcgcttcggg
     3241 aaccagcacc gatcgtgcaa ccggctccgc tcgtgtcttg acgatcatcg tctgtccgga
     3301 aatcagcgcg ccaggaccgt gcccggtatg aacggtagta acaccgaagc cgcgaaccca
     3361 ttccacgaga cgttcccggg cgttataggc gtccatggcc cgaaggtccg gctgtatggg
     3421 cgccgaagct tcgcgctggt cctggtcgtg tggctggttc aggtatccac cgagcccgac
     3481 taccgagtgc gcatcgatga gcccgggtgt tactacggcg gccttgacgg tccggtatcc
     3541 ggcggggatc cggacggatg aggcgggacc aacgctctcg atcttgccgt tgcgaaccag
     3601 cacgactcca ttcctaatcg gcgcacccgc ggaggtatgg acggttccgc ccacgacggc
     3661 gagctgggca gcggcgaccg aatacagagc cagcacgccg gtgaggaggc tgaggcaaat
     3721 tctcattgtt cgaactcctc cacgtagaag atctccgagc cttgatcgtg gctggcgcca
     3781 aaaccaccta cggcatacaa cctgtccttc ggatccgcgc ggtcgaagac cttcgtgccg
     3841 tcgacccagg tttccagcac tttcgtgtac acgctcaacg gatcgccgga caagatcacc
     3901 aggtccgcat ccttgcccgc ttcaagcgac ccgactctgt cttccaggcc cagcatcatg
     3961 gcattcgcca tcgtgactcc gaacagcgcc ttcccgcgcg acatgccggc gcgtaccgca
     4021 agcgcggggc tgcgaagaaa caggcgcgag tcggtgatgc catcgtcggt gtgaaaccca
     4081 accagcgcgc ccgctttttc gagtgcgcct ccggtt
//
LOCUS       DASTMK010000469        10985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_25530, whole genome shotgun sequence.
ACCESSION   DASTMK010000469 DASTMK010000000
VERSION     DASTMK010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10985
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25530"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1545
                     /locus_tag="VFQ79_19680"
     CDS             49..1545
                     /locus_tag="VFQ79_19680"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0141953.1"
                     /translation="MRPATTAELQECLRIANRLKVPVYPISSGMNWGYGSRLPSSDGC
                     VVIELSRMNRILEFDEDLGYVTVEPGVTQQQLFDFLKERKSRLWMDATGAHPDCSLIG
                     NTVERGFGHTPYADHFANSCGLEVVLPTGEIVETGFARFEGAKAAPAYRWGVGPTLDG
                     MFSQSNLGIVTRISIWLMPIPEHFEAYSFQCSDGALAAVIDALRPLRMDGTLRSAVHI
                     GNDYKVLSARQQYPWDESAGETPLSAGNINPFRKKLHFSAWNGFGALYGTRAQVAESK
                     RLLKRALAGKTADLKFLNDRKIRLMARLAKPLGLLTGWDINGILEVVEPVYELMRGVP
                     TDKPLSSAYWRKRTPVPSKMDPDRDRCGLLWCSPVTPTHGAEAQRLVQLSCDLVLSHG
                     FEPIISLTLITERAIACIISLSYDRDIDGEDARAMACYKALLRTAMEAGYYPYRLGVQ
                     SMWAMESGNGFDQLLRSIKDVIDPNGILAPGRYEPPRQPTPQSYSQSA"
     gene            1685..2413
                     /locus_tag="VFQ79_19685"
     CDS             1685..2413
                     /locus_tag="VFQ79_19685"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0141954.1"
                     /translation="MTKTAWTLVVVLSLLILPTLPLAAAPLSGSLNISGSWRISQDAI
                     DFFPPDGGPDGIFFVEPASSGDFASIADTQGKSLDLDSSPGGQPVGSDFLLSNFLLFD
                     AQPGWRFDLTFIQPGVFSSADCATVPAAGGQICTPAFPPPKSPFNLINASANSSSATF
                     NVRGFAYNAANEKSIFVGTYTTQFENRSYQSLLNDIVAGIPVEKSYSASFTLTPIPEP
                     GTLSIMLIGTGLLALGTLARRVRR"
     gene            2507..2812
                     /locus_tag="VFQ79_19690"
     CDS             2507..2812
                     /locus_tag="VFQ79_19690"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEU0141955.1"
                     /translation="MPIKPSDSEEEYFARQEAERRRKIAEERQAKIQDEERERERSLH
                     FMKCPKCGMQLEEIAFGDIRVDKCFSCEGIWLDKGELEGLQSKEPGFVGRLFKAFRT"
     gene            complement(2809..2967)
                     /locus_tag="VFQ79_19695"
     CDS             complement(2809..2967)
                     /locus_tag="VFQ79_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141956.1"
                     /translation="MNEPKLSDEIRKMEAEPLLPIEKKLIAWSLITGVVLLGVLIWLS
                     RTFFDPAA"
     gene            complement(2983..4422)
                     /locus_tag="VFQ79_19700"
     CDS             complement(2983..4422)
                     /locus_tag="VFQ79_19700"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEU0141957.1"
                     /translation="MVSTGRRISEDWLAVWIGVFIFLLSIGLLLGADLLGWAVTASVW
                     TDISTTLRPVSKAYAALPGLVSLVLTYVFLLAVMTAGAMALGANVKRFLSGFTVIFFL
                     SYLCWIAGAWAYIAATPDKLDTFGIGWSLNMTSEAGFIIALLAGLFIGNFLPRLASGI
                     QEAVRPELYIKTAIVILGGFLGVTAAEQLGLATSVMFRGLCAIIEAYLIYWAIVYFVA
                     RKYFGFSREWSVPLASGISICGVSAAIATGSAIRARPVVPVMVSSLVVIFAVVELLLL
                     PWMANHFLYQEPLVAGAWMGLAVKTDGAAVASGAIAESLILARAAEVQGIHYQPGWIV
                     ATTTTVKVFIDVFIAVWAFILAWIWSTKIERRAGERMRLADIWHRFPKFILGYMGTFV
                     LMLLLGLFARDLSAAGRAAMGEANVFRGIFFVMTFFTIGVVSNFRKLWEEGIGRLALV
                     YILCLFGFIIWVGLLISWLFFQGVKPPVV"
     gene            complement(4479..6656)
                     /gene="glgB"
                     /locus_tag="VFQ79_19705"
     CDS             complement(4479..6656)
                     /gene="glgB"
                     /locus_tag="VFQ79_19705"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU0141958.1"
                     /translation="MTHEEIEAIVGGYHGDAFRVLGPHGTGEPGQRSRWEVRAFLPQA
                     EHAEVLLDGSSAPMEKMHPQGFFVARLDGDQRHYLLRIRKWNGERSEFEDVYRFPPLL
                     TDFELHLVGEGTNFESYRTFGAHLTEIDGVQGTRFAVWAPNAIVVSVAGDFSEWDSRR
                     YPMRLRTGGIWELFIPGVKEGFNYKYDIRSKFRGYHQQKADPYALQSEAPPKSASIVC
                     DIGRYEWNDAAWVENRAQTNWLDKPVSIYEVHLESWLRGPQNQTLTYRELAGSLVPYA
                     KKLGFTHIQLLPVHEHPYSGSWGYQVTGYYAPTARFGTPLDFKYFVDCCHQEGLGVII
                     DWVPGHFPKDAHGLAFFDGTALYEHEDPRKGEHRDWGTLIFNYGRNEVREFLISNALF
                     WLKEYHIDGLRVDAVASMLYLDYSRKPGEWVPNIYGGNENLEAISFLRSFNELAHQVP
                     GAITIAEESTSFPGVSKPVYLNGLGFTMKWNMGWMHDMLDYFSREPVHRKYHHNNITF
                     SMLYAFTENFVLPISHDEVVYGKRSLLGKMPGDEWQRFANVRAFLGYMYAHPGKKLLF
                     MGVEIGQYEEWNHNAGVRWELLDFPVHRQLQHLVKELNRVYGEQPALYEVDYHWSGFE
                     WIDFHDVEGSSISFVRRAQDPDNLIVVCCNFTPVPREKYRVGVPAEGFYQEILNTDAE
                     CFGGGNMGNGGMVSSEPAPFHGREHSISITLPPLATVLFRRRV"
     gene            6655..7620
                     /gene="add"
                     /locus_tag="VFQ79_19710"
     CDS             6655..7620
                     /gene="add"
                     /locus_tag="VFQ79_19710"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU0141959.1"
                     /translation="MLFIMAMEPDFYRELAKAELHLHLEGSIAPATLRELAPHVPPEE
                     IDRRYRYADFSGFIESYVWVNSFLNTPDDYAFVARRLLESLAAENVCYAEINLSAGVI
                     LWKQMNLARVYEAVAAEAACSPVEVRWILDAVRQWGPEKAMEVARFAVERRGDGIVAF
                     GIGGDESRGHCDWFSDVFAFAKAQGLKLAPHAGETCGPESVWSALRQGADRIGHGIRA
                     AGDADLMRELRERDIPLEICISSNVATGATPSLDAHPVRKLYDAGVPIVLNTDDPPMF
                     HTTLSREYEIAATRFGFSRAELSGLAANSFRYAFVKQEPRPVPNR"
     gene            complement(7586..9025)
                     /locus_tag="VFQ79_19715"
     CDS             complement(7586..9025)
                     /locus_tag="VFQ79_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0141960.1"
                     /translation="MQHIPRRGFLRAFGASSVLGFPALLAAPKSTDVRIKEVKFGYED
                     YLYRTPIKFGGTPVDRVTLLNVDCVVETANGRSAKGFGSMPLGNIWAFPSKDMPYDVT
                     LNAMKGLSERVAKITADYNETRHPVDINVALEPLYLSAAEEVSRRQKLKAPIPKLATL
                     VVASPFDAAIHDAFGKLHRRSAYDIYGSDLMTHDLSHYLGPEFKGEYLQQYVLKKPKS
                     RMPLYHLVGALDPITESDIQKRLNDKLPETLPEWIEYSGLTHIKIKLNGDDLNWDVNR
                     VLAVDRATAETQRKRGVKTWHYSADFNERCPNVTYLLDFLSQVKEKAPAAFERVQYIE
                     QPTARDLKAHRSNVMHRASKLRPVVIDESLTDLENLLLAREMGYTGAALKACKGQAQA
                     LLMAAAAQKYKMFLCVQDLTCPGASLIHSAGIAAHVPGVAAIEANSRQYCPAANEAWE
                     KRFPGIFDVRDGTVHTELINGLGLGAVPA"
     gene            9203..9967
                     /locus_tag="VFQ79_19720"
     CDS             9203..9967
                     /locus_tag="VFQ79_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0141961.1"
                     /translation="MFEQALLDGAVRTNRPWTTLVSLAGQTGLVGILLLVPLIFTDAL
                     PRAELSNLFMMPAPPPAAPPPPPPAEIMQKIAKVIPRQFVDSRLMAPRAVPDKIATII
                     EEESLPAVSGVVGGVEGGVPGGMLHGVIGAVVDAASSHTTALPPPPPPVAVQKPTPPQ
                     RIKVGGNVQEAKLIKRVIPTYPPLARQARVGGIVQLIGVITKEGVVAQLQVVSGHPLL
                     VGAALDAVRQWVYEPTLLNGDKVEVIAPITVNFILQ"
     gene            complement(10006..10554)
                     /gene="hpt"
                     /locus_tag="VFQ79_19725"
     CDS             complement(10006..10554)
                     /gene="hpt"
                     /locus_tag="VFQ79_19725"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU0141962.1"
                     /translation="MSKPAPSGIERILFTEEQIRHRIGEVAAEISERYRGRHLKLIAV
                     LKGSVFFLTALAREIDIPVKMDFLAISSFGSQKAGGGVVRISKDLDDVIQDEDVLLVE
                     DIVDTGLTARYLLQTLAGRGPNSISICTLLDRTSRRIVQVPVEFRCFEIPDRFVVGFG
                     LDYRQLYRNLDYIAVMNPEKAG"
     gene            complement(10551..>10985)
                     /locus_tag="VFQ79_19730"
     CDS             complement(10551..>10985)
                     /locus_tag="VFQ79_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141963.1"
                     /translation="HGLLGASAEAQLQFQVRLELAKHGITPIGHAELKRRAFGHPSEE
                     VFANVLDFYRVAWKYEPKSFPIRWDKNGNVIEAFTPDFYLPEFNLYVELTTMKQSLVT
                     RKNRKVKLLKTLYPDVNIQVFYQKDFQDLVFKYGLGERVTKQ"
BASE COUNT         2368 a         3181 c         3134 g         2302 t
ORIGIN      
        1 cctttcaatg taagcacatt cgcaagtccc ttccgtacac ctgccatcct gcggccggca
       61 actaccgctg aactgcagga gtgcctgcga atcgccaatc ggctcaaggt gccggtttat
      121 cccatcagca gcggtatgaa ctggggctac gggtctcgtc tgccttcctc ggatggttgc
      181 gtcgttatcg aactgtctcg aatgaaccgc attctcgaat tcgatgaaga tttggggtat
      241 gtcactgtag agccaggggt tactcagcag caactgtttg atttcctcaa agagcgaaaa
      301 agccgtctat ggatggacgc caccggcgcg caccccgatt gcagccttat cggcaacacc
      361 gtcgagcggg gctttggcca tactccttat gcggatcatt ttgcgaactc ctgtgggctg
      421 gaagttgtac tgcccaccgg ggaaattgta gaaacggggt tcgcgcgttt cgagggggcc
      481 aaggcagcgc cggcgtatcg ttggggtgtc ggaccgacgc tcgatggaat gttctcccag
      541 tccaatctgg gcattgttac tcgcattagc atctggctca tgcctatacc cgagcacttc
      601 gaggcatact ctttccagtg cagtgacggg gcccttgcgg ccgttatcga cgccctgcgc
      661 cctttacgta tggatggcac gctccggagc gccgttcata tcgggaacga ttataaagtg
      721 ctttccgccc ggcaacagta tccctgggac gaatcggcgg gtgagacgcc gctttcggcc
      781 gggaatataa atccgttccg gaaaaagttg catttcagtg cctggaacgg ctttggcgct
      841 ctttacggaa cccgggccca agtggctgaa tcgaaacggc tgctgaagcg tgcgctggcc
      901 ggcaaaacag cggatctgaa gttcctgaac gatcggaaga tccggctcat ggcccgcctg
      961 gcgaaaccct tgggcctcct gaccgggtgg gatattaacg gcatcctaga ggtggtggaa
     1021 cccgtttacg aactgatgcg cggcgtgccg accgacaaac cgctatccag tgcctactgg
     1081 cgaaaacgta cccccgttcc gtcgaaaatg gatccggacc gggaccgttg cggtctcctg
     1141 tggtgcagtc ctgtgacacc cacacacggg gctgaggcac agcgcctggt ccagctgtcc
     1201 tgcgatttag tactatccca tggttttgaa ccgattatat ccctgaccct catcactgaa
     1261 agagcgatcg cgtgcattat ctccttgagt tatgaccggg atatcgacgg cgaggatgcc
     1321 cgggcgatgg cctgttataa ggctttgctg cgcactgcga tggaggctgg gtactatccc
     1381 tatcgtcttg gggtacaatc catgtgggct atggaaagcg gcaatggctt cgaccaactg
     1441 cttcgctcca taaaggatgt tattgatcct aatgggattt tggcgccggg acgttatgag
     1501 ccgccacggc agcccacccc tcagtcctat tcccagtccg cctaaggtgt aaataggctt
     1561 gtaacttagt cttttattga caccacgggc aatctgaaat accataagaa acagttcgtc
     1621 gcgtgacgta tccgcgagct ctcgtgttca aagtacagtt gtggaatttc ttcggaggag
     1681 gataatgaca aaaaccgcgt ggacgttagt agttgttttg agtttgctga ttctgcctac
     1741 gctgccactt gcggcagcgc cgctatccgg gtcgttgaat atcagcggat cctggcggat
     1801 cagccaagat gccattgact ttttcccgcc tgatggtgga ccagatggga tcttctttgt
     1861 agagcctgca tcgagtgggg atttcgcctc catagccgat acgcagggaa aatctctcga
     1921 tctcgattct tcaccgggtg gacagccggt tgggagcgat tttctgttga gcaacttcct
     1981 gctgtttgac gctcagccag gctggagatt cgacctgact ttcattcagc caggggtgtt
     2041 cagttccgca gattgcgcga ctgtgccggc tgccgggggc cagatttgta cgcctgcttt
     2101 cccgccgccc aagtcgcctt ttaacctgat caatgccagc gccaattcat cgtcggcgac
     2161 tttcaacgtc aggggctttg cctacaacgc tgcaaacgag aagtccattt tcgttggcac
     2221 ctacactacc cagttcgaaa accgctccta ccagtcgttg ctgaacgata tcgtcgcggg
     2281 cattccggta gagaagtcct actccgcttc gttcaccctt acgccgattc cggagccggg
     2341 tactctatcg atcatgctga tcggtaccgg gctgttggct ctcggcactt tggctcgccg
     2401 cgtccggcgg taagctgaag ggcatccgtt tcaaccacgg ctcccggcgt tgagcgccgg
     2461 gggccgtttt tgcgttagcg gcacggaatc gctagtagag gaacatatgc ctatcaagcc
     2521 tagtgacagc gaagaggagt atttcgcccg gcaggaagcg gagcgaaggc gaaagatcgc
     2581 agaggagcgg caagcgaaga tccaggatga ggagcgcgag cgggagcgct ctcttcactt
     2641 catgaaatgc cctaagtgcg gaatgcagct agaggagatc gctttcggcg atattcgcgt
     2701 cgacaagtgc tttagctgcg aagggatctg gctcgacaaa ggtgagctcg aagggctcca
     2761 gtcgaaggag cccggcttcg taggcaggct gttcaaagca ttccggactt aagccgccgg
     2821 gtcgaaaaac gttctgctaa gccagatcag cactcccaac aacaccaccc cggtaatcag
     2881 gctccaggcg atcagcttct tttcaattgg cagaagcggc tctgcttcca ttttcctgat
     2941 ttcgtcggat aatttcggtt cgttcacaag tgctcccctc ttctatacca ccggcggctt
     3001 cacgccctgg aagaacagcc aggagatcaa caagccgacc cagatgatga atccgaacag
     3061 acaaagaatg tagaccagtg caagcctgcc aattccctct tcccaaagct tccggaagtt
     3121 ggagaccacg ccaatggtaa agaacgtcat aacaaagaaa atgccgcgga aaacgttggc
     3181 ttcccccatg gcggcgcggc ctgccgcgct cagatcccgc gcaaacaagc ctagcagcag
     3241 catcagaacg aacgtcccca tatagccgag aatgaacttg gggaaccggt gccagatatc
     3301 ggccagccgc atacgctccc ccgcgcgccg ttcgattttg gtggaccaga tccaggccag
     3361 gatgaacgcc caaaccgcga taaagacatc gatgaacacc ttcaccgtgg tggttgtggc
     3421 gacaatccat ccaggctgat agtggatacc ctgcacctcc gccgccctgg cgagtatgag
     3481 cgattcggcg attgctccgc ttgccaccgc cgcgccgtcc gtcttaacgg caagccccat
     3541 ccacgctccg gccaccagcg gttcctggta gaggaaatgg ttggccatcc acggcagcag
     3601 gagcagttcc acaacggcga aaatcaccac cagagacgac accatcaccg gcaccaccgg
     3661 gcgcgcacgg attgccgaac cggtggcaat ggcggccgaa acgccgcaga tggagatacc
     3721 cgaggctagc ggcaccgacc actcccggct gaatccgaaa tacttgcgcg ccacgaaata
     3781 cacgatggcc caataaatca ggtaggcctc aatgatggcg cacagcccgc ggaacatcac
     3841 cgacgtggca agcccgagtt gctcggcggc cgttactccg agaaatcccc ccagaatcac
     3901 gatcgccgtc ttgatgtaga gttccggacg caccgcctcc tggatgccgc tggcgagccg
     3961 cggaagaaag ttaccgataa acagcccggc aagcagcgcg atgataaacc cggcttcgga
     4021 cgtcatgttg agcgaccagc cgatgccgaa tgtgtcgagt ttgtcgggtg tggccgcgat
     4081 gtatgcccag gcgcccgcaa tccagcatag atagctcagg aagaagatga cggtgaagcc
     4141 actgagaaat cgcttcacgt tcgcacccag cgccatcgcg cccgcggtca tcacggcgag
     4201 aagaaaaaca taagtaagaa ccaacgaaac gagtcccggt agtgccgcat aggccttgga
     4261 tacaggccgc agcgtggtgg aaatatccgt ccacacggac gcggtcaccg cccagccgag
     4321 cagatcggcg cctaaaagca agccgatcga aagcaaaaaa atgaatacac caatccagac
     4381 agcaagccag tcttcggaaa tacgacgccc agtcgaaacc atccagccta ttgtgcaaca
     4441 tcgtgcgttg gactttcagg tgtactggtt cggtgccgtt aaacacgacg ccggaacagg
     4501 actgtcgcaa gcggcggaag tgtgatcgaa atgctgtgct cacgtccgtg aaacggcgcg
     4561 ggctcagacg acaccatccc accattaccc atgtttcctc caccgaaaca ttccgcatcc
     4621 gtgttgagga tctcctgata gaaaccttcc gccggtacgc cgacgcgata cttctcacgc
     4681 ggaacgggag tgaagttaca gcaaaccaca atcaggttat ccggatcctg cgcgcggcgg
     4741 acgaaggata tgctggagcc ctccacgtcg tgaaaatcga tccactcgaa cccactccag
     4801 tgatagtcga cttcgtagag cgcgggctgc tcgccgtata cgcgattcaa ttccttcacc
     4861 aggtgctgca gttggcgatg gacggggaaa tccagcagtt cccagcgcac acccgcattg
     4921 tgattccact cctcgtattg cccgatttcg acgcccatga atagcagctt cttgccgggg
     4981 tgagcataca tgtatccgag aaaagcgcga acattggcga atcgctgcca ctcatcgccc
     5041 ggcatttttc ccagcagaga gcgtttcccg tagacaacct cgtcgtgaga gatcggcagg
     5101 acaaagttct cggtaaaggc gtagagcatg ctgaacgtga tgttgttgtg atgatacttg
     5161 cggtggaccg gctctctcga aaaataatcc agcatgtcgt gcatccaccc catgttccac
     5221 ttcatagtga acccgagccc gttcaggtaa accggtttcg atacgccggg aaaagaagtg
     5281 gattcctccg cgatcgtaat cgctcctgga acctggtgcg caagttcatt gaagcttcgc
     5341 aggaacgaga tcgcttccag gttttcgttg cctccatata tgttgggaac ccactcgccg
     5401 ggctttcgcg agtagtccag gtacagcatg gacgctacgg catctacgcg gagaccgtcg
     5461 atatggtatt ccttcaacca gaacaatgcg ttcgaaatga gaaattcccg cacttcgttc
     5521 cggccatagt taaagatcag ggtgccccaa tcgcggtgct cgcccttgcg gggatcttcg
     5581 tgctcgtaaa gggcagtgcc gtcgaagaat gccagcccgt gcgcatcttt cgggaaatga
     5641 cccggcaccc agtcgataat gacgccgagg ccttcctggt ggcagcaatc gacgaaatac
     5701 ttgaaatcca gcggtgttcc gaaccgggcg gtgggagcgt aatatccggt cacttggtag
     5761 ccccacgatc cggaatacgg gtgctcatgc accggaagca actgaatgtg ggtgaagccc
     5821 agtttcttcg catacggtac cagtgatccg gccagttcgc ggtaagttag cgtctggttc
     5881 tgcggcccgc gcagccacga ttccagatgc acttcgtaaa tggaaaccgg cttgtccagc
     5941 cagttcgtct gcgcacggtt ctctacccat gcggcatcgt tccattcgta gcgcccgatg
     6001 tcgcagacaa tggatgcgga tttgggagga gcctccgact gcaacgcgta tgggtccgcc
     6061 ttctgctgat gatagccccg gaacttggac cggatatcgt atttgtaatt gaacccttct
     6121 ttgacgcctg gaatgaacag ttcccagatg cctccggtgc gcagccgcat aggatatctg
     6181 cggctgtccc attccgagaa gtcaccggcc actgacacga caatcgcatt cggcgcccag
     6241 acggcaaaac gagtaccctg gacaccgtcg atctccgtaa ggtgcgcacc gaacgtacga
     6301 tagctctcga agttcgtacc ttcgcccacc aggtgcagct caaaatccgt cagcagggga
     6361 ggaaagcgat acacgtcttc gaattcggac cgctcaccat tccactttct gattcgaagc
     6421 aggtagtgcc gctggtcacc gtccagcctg gcgacaaaaa agccctgcgg gtgcatcttc
     6481 tccataggcg cggaagagcc gtccaggaga acttcggcgt gttccgcctg aggcaggaag
     6541 gcgcgcacct cccagcggct gcgctgaccg ggttcgccgg ttccatgcgg acccagcacc
     6601 cggaatgcat cgccatgata tccccccact atcgcttcga tctcttcgtg cgtcatgctt
     6661 ttcattatgg caatggaacc ggatttctac cgggaattgg cgaaggcgga attgcatctt
     6721 catctggaag gttcaattgc accggcgacg ctgcgcgaac tggcgccgca tgtgccacct
     6781 gaggagatcg atcggcgcta tcgctatgcg gacttcagcg ggttcatcga aagctacgta
     6841 tgggtcaact ctttcctgaa cactcccgac gattatgcct ttgtcgcgcg ccgtctgctg
     6901 gagagcctcg ccgcggaaaa tgtctgttat gccgagatca acctctcggc cggggttatt
     6961 ctctggaagc agatgaacct ggcaagggta tacgaggcgg ttgccgcgga agcagcgtgt
     7021 tctcccgtcg aggtccggtg gatcctagac gcggtccggc agtggggacc ggaaaaggca
     7081 atggaagtgg cacgtttcgc cgtagaaaga cggggggatg gcatcgtcgc cttcggcatc
     7141 ggcggtgacg aaagccgcgg ccactgcgat tggttctcgg acgtattcgc gtttgccaaa
     7201 gcgcagggcc tgaaacttgc acctcacgcg ggcgagacgt gcggtccgga gtccgtgtgg
     7261 agcgcgctgc ggcagggcgc cgatagaatc ggccacggca ttcgcgcggc gggagacgcg
     7321 gatctgatgc gcgagctgcg ggaacgcgat atcccactcg aaatctgcat ttcgagcaat
     7381 gtggcgacgg gcgcgacgcc ctcgctcgac gcgcatccgg tccgtaaact ctacgatgcg
     7441 ggcgtcccta tcgttctgaa caccgatgac ccaccgatgt ttcacaccac gctgagccgc
     7501 gaatacgaaa tcgcggcgac gcggttcggg ttctcccgcg cggagttatc cgggctggcg
     7561 gcgaacagct tccggtatgc atttgttaag caggaaccgc gcccagtccc aaaccgttga
     7621 tcaattccgt gtgtaccgtc ccgtcgcgca cgtcgaagat acccgggaag cgcttctccc
     7681 aggcttcatt cgcggccggg cagtactggc ggctgttcgc ttcgatggcc gcgacgcccg
     7741 gaacgtgcgc cgcaatgccg gcggagtgga tgagcgaagc cccggggcat gtcaggtcct
     7801 gaacacagag gaacattttg tacttctgcg cggccgcagc catgagaagc gcctgtgcct
     7861 gtcctttgca cgcctttagc gccgcgcctg tataccccat ctcgcgcgcg agcagcaggt
     7921 tctccagatc cgtcaaggat tcgtcgatca ccaccggccg cagttttgaa gccctatgca
     7981 ttacgttgga gcgatgcgcc ttgaggtccc gcgccgtcgg ctgttcaata tactggacgc
     8041 gttcgaaagc agcgggagct ttctccttca cctggctcag gaaatcgaga agataggtga
     8101 cgttcgggca acgctcgttg aagtccgccg aataatgcca ggtcttcaca ccgcgtttcc
     8161 gctgcgtctc ggcagtcgca cggtccactg ccagtacgcg gttcacatcc cagttcaagt
     8221 cgtctccgtt cagcttgatt ttgatgtggg tcagcccgct atattcgatc cactcgggta
     8281 gcgtctccgg aagcttgtcg ttgaggcgct tctggatatc cgattccgtg atcgggtcga
     8341 gcgctcccac gagatgatag agcggcatcc ggctttttgg tttcttcaaa acatactgct
     8401 gaagatactc gcctttgaat tccggcccga gataatgcga aaggtcatgc gtcatcaggt
     8461 cggatccata gatgtcgtaa gcgctgcgcc ggtgcagttt tccgaacgcg tcgtgaatgg
     8521 ccgcatcgaa cgggcttgcc acgacgagag tagccagttt cggaatagga gctttcagct
     8581 tctgccggcg cgacacctct tccgcggcgc tcagatacag cggctccagc gcaacgttga
     8641 tgtccacagg atgccgtgtc tcgttgtaat cggcagtgat tttggccacc ctctccgaca
     8701 ggcccttcat ggcgttcaag gtgacgtcat acggcatgtc ctttgaggga aacgcccaga
     8761 tgtttcccag aggcatggag ccaaagccct tcgcactgcg gccattcgca gtctcgacaa
     8821 cacaatcgac attcagcaag gtcacgcggt ccacgggtgt gccgccaaac ttgatgggcg
     8881 ttcgatacag gtagtcttcg tatccgaact tcacttcctt gatgcgcacg tcagtggact
     8941 tgggcgctgc gagaagcgcc gggaatccca aaacggacga cgcgccgaaa gcgcgaagaa
     9001 agccgcgacg aggaatatgc tgcataggct ccattatcat atggaactcg catgccaatg
     9061 ctggtgtcta ttagcgtgta aaaggaaggc gcaacatgat cttccaaatg gtctcgcgag
     9121 cctgaacgtc gccggcgccg ccggcgggaa ctttcaagaa atctgaatcg gtacgtgacg
     9181 aggagaaagg agtggtgtgt ctatgttcga gcaggcttta ttggatgggg ccgtcagaac
     9241 caacaggccg tggactacgc tggtttcgct ggccggacaa acgggattag tcggcatcct
     9301 gttactggtg ccgctgatct tcaccgatgc tttaccgcga gcggagcttt ccaacctgtt
     9361 catgatgccg gcacctcctc ccgccgcacc tccaccgcca ccgccggcgg agatcatgca
     9421 aaaaatagcc aaggtgattc cgcggcagtt tgtcgactct cgtctcatgg cgccacgtgc
     9481 ggtgccggac aagattgcca cgatcattga ggaagaatcg ctcccggccg tctccggcgt
     9541 cgttgggggc gtcgagggcg gtgttccggg tggaatgctg catggagtca tcggagccgt
     9601 tgtggacgcg gcatcttcgc acacaaccgc gctccctccg ccgccgccac cggttgcggt
     9661 gcagaagccc acaccacccc agcgcatcaa ggtcggcggc aacgtgcagg aagcgaagct
     9721 gattaaaaga gtgatcccaa cctatccgcc actggcccgc caggcgcgcg tcggcggcat
     9781 tgtgcagcta attggtgtga tcacgaaaga gggagttgtg gcgcagctac aggtcgtcag
     9841 cgggcatccc ttactggtag gcgcggcgct cgatgccgtg cggcaatggg tttatgaacc
     9901 gacgctgctg aatggcgaca aagtggaggt gattgcacca atcaccgtga atttcatcct
     9961 ccagtaacac tccggaggga ggcggtacgg agccgcctcc ctctatcagc ctgccttctc
    10021 cgggttcatc accgcgatgt aatccaggtt gcggtaaagc tgccggtagt cgaggccgaa
    10081 gccgacgacg aaccggtcgg gaatttcgaa gcagcgaaat tcaaccggca cctgcacgat
    10141 gcggcgggag gtccggtcga gaagcgtgca gatggagatg gaattcggcc cgcgtccggc
    10201 gagagtttgc agaagatacc gggcagtgag gccggtatcc acaatatctt cgacgagcag
    10261 cacgtcttcg tcctggatca catcatcgag gtctttggaa atcctgacaa ctcccccgcc
    10321 cgctttctgc gatccgaagc tcgaaatggc gagaaaatcc attttcaccg ggatatcgat
    10381 ttcccgcgcc aatgcggtta aaaagaaaac cgatcccttc agtacggcga tcagcttcag
    10441 gtgacggcct ctgtagcgct ccgagatctc agcggcgacc tcgccgatcc ggtgccggat
    10501 ctgttcttcg gtgaaaagga ttcgttcaat accggaagga gcgggcttgc tcattgcttc
    10561 gtaacccgct cacccaagcc gtatttgaat acgaggtcct gaaaatcctt ctggtaaaac
    10621 acttgtatgt tcacgtcggg atatagcgtc ttcagcaatt tcactttacg attctttcgg
    10681 gtgacaagcg actgcttcat cgtagtcagc tcgacgtaga ggttgaactc gggcaggtag
    10741 aaatcgggcg taaacgcctc gatgacgttg ccgttcttgt cccaacgaat cggaaagctc
    10801 ttgggctcgt atttccacgc tacgcgatag aaatcgagga cgttggcaaa aacttcctcg
    10861 ctcgggtggc caaaggctcg ccgcttgagc tcggcgtgtc cgatgggagt aattccgtgt
    10921 ttagcgagtt cgaggcgcac ctggaactga agctgcgctt cggcggaagc gcccagcaat
    10981 ccgtg
//
LOCUS       DASTMK010000470         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_376018, whole genome shotgun sequence.
ACCESSION   DASTMK010000470 DASTMK010000000
VERSION     DASTMK010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376018"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFQ79_19735"
     CDS             <1..807
                     /locus_tag="VFQ79_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0141964.1"
                     /translation="SILRFENVTVTLDEIAVLDSITFEMRKNDTCVILGAAGSGKTVL
                     LKTAIGLIKPSTGKVFLFDTDITELDEQEMFDLRAKTGVLFQEGALFDSLTIEENVAY
                     PLVNQRAAKRRKREIRELVEESLRFVELSHTLDKFPSELSGGMRRRVGIARAIVTEPP
                     LVLYDSPTAGLDPITANTIVTLIIKERDVKKTTTVVVTHRYQDGHLLANFRYNPGNGQ
                     VEPVTGNGHTDTLKTRFLVLRQGRLTFVGDQQQLEASADPYVSKFVKREI"
     gene            873..1883
                     /locus_tag="VFQ79_19740"
     CDS             873..1883
                     /locus_tag="VFQ79_19740"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0141965.1"
                     /translation="MIIVATLVFLLTGSKDIFATEATLYTFLNDSAALTEGAIVRLNG
                     IPVGSVSKISLTGSMDPNKMIRLDLKVREDLLSHIPVDSLASISAENVLGTKFINIKK
                     GQSAEIVKPGGTIGSLDTREFDEVVQQGYSLLTALRGILERVDKIVAVVEAGKGSIGK
                     LIVDDEFYDRLVSIVAEGQKISAALASDRGTIGKLIYDDALYRDVRNSLARLDTLIEG
                     LQRGEGTAGRLLKDPALYEEARQTIGEVRSLLNDLNAGKGSAGKFLKSDELHDRIAGT
                     ITRVDNMLDRLNAGQGTLGQLLVNPQLYESVNGATRELNGLLQDFRANPRKFLHIRLS
                     LF"
     gene            1880..2695
                     /locus_tag="VFQ79_19745"
     CDS             1880..2695
                     /locus_tag="VFQ79_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU0141966.1"
                     /translation="MKRLVINADDFGFTRDVNDGIVEAHRRGILTATTLMANGGAFED
                     AVRLARENPALDVGCHLVLVSGASVADPNRRLPVTVGGMLRAIVSRRLRPFEELRAQV
                     LRITGAGIRPTHLDTHKHTHLFPPVLNAVAKISREFGIPWVRRPFDFPLNGSAARPPL
                     SVRLTSRCLGIVRGRFHRTLDAHGCRTTDHFAGFQITGHVGVEEIVQLIRKLPEGLTE
                     FMCHPGRCGPELMKARTRLKESRERELHALTAPEVRDALDRAGVELVAYRDLL"
     gene            complement(2697..2879)
                     /locus_tag="VFQ79_19750"
     CDS             complement(2697..2879)
                     /locus_tag="VFQ79_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141967.1"
                     /translation="MKNHRIIGVMARLDLHFKVVIDMDDAEDPEKLAAEICRQISRVY
                     GVRSAELSNYISHTEE"
     gene            2880..3533
                     /locus_tag="VFQ79_19755"
     CDS             2880..3533
                     /locus_tag="VFQ79_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141968.1"
                     /translation="MLCKICETRRPRRYCPGVMGDICSLCCGTEREQTVDCPLECEYL
                     QDARRHEKLPDIDPNEFPNKDIEVTERFLREHEHLLLQAAGSLLRGTMETRGAVDQDV
                     REALDSLIRTYRTLESGLYYETMPTNPFAASIHHQLQEAVKVFREQLARELGLNTVRD
                     TDVLGVLVFLQRLELQHNNGRKRGRAFIDFLRQHFPMPPSVASGAPGAAPDSPIILG"
     gene            complement(3540..>4059)
                     /locus_tag="VFQ79_19760"
     CDS             complement(3540..>4059)
                     /locus_tag="VFQ79_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEU0141969.1"
                     /translation="AGKDAYYEFEINAFNTIYEVFWIWKDMFRPGSPYNVPEWDPKGR
                     NVATLSGVGGHVHPRGERYGYLDWDFPGLRHAVHINGTLNKRDDVDEGWTAEIAMPWK
                     GLKWLADGRALPPKNGDVWRIDCSRFQHIGPHGERLDPAAGWTWNRHGHYDSHIPETF
                     TYVQFSTDPVRR"
BASE COUNT          844 a         1145 c         1235 g          835 t
ORIGIN      
        1 tccatacttc ggttcgagaa cgttaccgtg accttggatg aaatcgcggt gctggacagc
       61 atcacttttg agatgcgcaa aaacgacacc tgcgtcatcc tgggcgccgc gggaagcggc
      121 aaaaccgttc tgctaaaaac cgcgatcgga ttgataaagc ccagcaccgg gaaggtattt
      181 ctgttcgata cggacatcac ggagctcgat gaacaggaga tgttcgacct gcgggcaaag
      241 accggtgtgt tgttccagga gggtgcgctg ttcgattccc tgacgatcga ggagaacgtc
      301 gcctatcccc tggtaaatca gagagccgcc aagcggagaa aacgggagat ccgggagctt
      361 gtcgaggagt ctctgcgctt tgtggagctg tcccacacac tggataaatt cccaagtgag
      421 ctgtccggtg gcatgaggcg gcgtgtcggc attgcgcgag ccattgtgac ggagccccca
      481 ctggtgcttt acgattcgcc cacggcggga ctcgacccta tcaccgcgaa tacgatcgtg
      541 acactcatca taaaagagag ggatgtgaag aaaaccacta ccgtggtagt tacgcaccgt
      601 tatcaggatg ggcacctgct ggcgaacttc cggtataatc ccggcaatgg ccaggtggag
      661 cccgtcacgg gcaacgggca tactgatact ttgaagaccc ggttcctggt gcttcggcag
      721 ggccgtctta cgttcgtggg ggaccagcag cagttggaag cgtccgcgga cccttacgtc
      781 tctaaattcg tcaagcgcga aatctgatta tgccttcagc gaggaaagtg gggtgggcgc
      841 agcttcgagt cggtttactg acactcacag ccatgatcat cgtggctact ttggtgtttc
      901 tccttaccgg atcgaaagat atcttcgcca ccgaggctac gttgtacacg ttcctgaacg
      961 attcggccgc gttgaccgaa ggcgccatcg tccggctgaa cggaataccg gtgggcagcg
     1021 tcagcaagat ttccttaacc gggtcgatgg atccgaataa aatgatccgg ctcgacctca
     1081 aagtccggga ggatctgctc tcgcacatcc cggtggattc gctggcttcc atcagcgcgg
     1141 agaacgtgct tggcacgaag ttcatcaaca tcaagaaagg gcagagcgcc gaaatcgtca
     1201 agccgggcgg aacgatcgga agcctggata cacgcgagtt cgacgaagtg gtgcaacagg
     1261 gctattcgct gttgactgca ctccggggaa ttctggagcg ggtggataaa atcgtagcgg
     1321 tcgtcgaagc aggaaaaggc agtatcggca agctgatcgt cgacgacgag ttttacgatc
     1381 gcctggtaag cattgtggcc gagggccaga aaatctccgc cgctctcgcc agcgatcgtg
     1441 gaacgatcgg aaaactgatt tacgacgatg cgctataccg cgatgtccgg aattcccttg
     1501 cgcgcctgga tacgttgatc gaagggctcc agcgaggcga gggtacggcc ggccggctcc
     1561 tgaaagatcc ggcgctttat gaagaggcaa gacagacgat tggcgaagtg cgctctttgc
     1621 tcaacgatct gaacgcgggt aagggctccg cgggtaaatt cctgaagagc gacgaactgc
     1681 acgaccgcat tgccggcacc atcacgcgtg tggacaacat gctggaccgg ttgaacgcgg
     1741 gtcagggcac gttgggccag ttgctggtga acccgcagtt gtatgaatcc gtaaatggcg
     1801 ccacgcgcga gttgaacggg ttattgcagg attttcgggc gaatccgcga aaatttctgc
     1861 atatcaggtt gagcctgttt tgaaacgcct ggtgatcaat gccgacgact tcggattcac
     1921 acgcgacgtc aacgacggga tcgtcgaggc gcaccgccgc ggtatcctca cggcaactac
     1981 cctcatggcc aatggcggcg ccttcgaaga tgctgttcgt ctcgcccgtg aaaacccggc
     2041 gctcgatgtc ggctgtcacc tggtgctggt gagcggcgca tcggtggccg atccgaaccg
     2101 gcgactgccg gtcacagtcg gtgggatgct gcgcgccatc gtcagcaggc ggctgcggcc
     2161 tttcgaagag ctgcgcgcgc aggtgctgcg aattaccggt gccggtatca ggccgacgca
     2221 tctcgacacg cataaacata cacacctgtt tccacctgtt ttgaacgcgg tcgcgaagat
     2281 ctcacgcgaa ttcgggattc cgtgggtgcg ccggccgttc gattttcctc tgaatggctc
     2341 tgccgccagg ccgccgctct ctgtccggct taccagccgg tgcctgggca tcgtccgcgg
     2401 gcgctttcat cgaactctcg acgcgcacgg atgcaggacg accgatcatt ttgccgggtt
     2461 tcagattaca gggcatgtcg gggttgagga gatcgtgcaa ttgatccgga agctgccgga
     2521 gggcttgacg gagttcatgt gccatccggg acgctgcggc ccggagctaa tgaaagcgcg
     2581 cacacgcttg aaagagagcc gcgaacgtga gcttcatgcc ttgaccgctc cggaagttcg
     2641 cgacgccctc gaccgcgcgg gagtggagtt ggtagcctac cgggacctgc tctgaatcat
     2701 tcctcggtgt gcgagatgta gttgctcagt tcggcggagc gcacgccgta gacgcgggag
     2761 atctggcggc agatctcagc cgcgagcttt tccgggtctt ccgcgtcatc catatcgatc
     2821 actactttga aatgcaggtc caggcgagcc attaccccga tgatacgatg gttcttcaca
     2881 tgctttgcaa gatctgcgaa acgcggcggc cccgccggta ttgtcccggt gtgatggggg
     2941 acatctgttc cctatgctgc ggaaccgaac gcgaacagac agtcgattgt ccgctggaat
     3001 gcgaatacct tcaggatgcg cgccgccatg agaaactccc cgatatcgac ccgaatgagt
     3061 ttcccaataa agacatcgag gtgacggaac gttttttacg ggagcatgag catctgctgc
     3121 tgcaagcggc aggcagcctg ctgcgcggca caatggaaac gcggggcgcc gtcgaccagg
     3181 atgtacgtga agcgctcgac tcgctcatcc gcacttaccg gacacttgaa agcgggctct
     3241 attacgaaac catgcccact aacccattcg cagccagcat ccaccaccag ttgcaggagg
     3301 ccgtgaaggt ttttcgcgag cagcttgcgc gcgagctcgg gctcaatacg gtccgggata
     3361 cagatgtgct cggagttctg gtgtttttgc aacggctcga acttcaacac aataacgggc
     3421 gcaagcgggg acgggccttt atcgatttcc ttcggcagca ctttccaatg ccgccttcgg
     3481 ttgcgtcagg ggcaccagga gcggcgccgg attcgccgat catcctgggc tgagggggcc
     3541 tatcgccgca ccgggtccgt ggaaaactga acgtaagtga acgtctcggg gatgtgcgag
     3601 tcgtaatgac cgtgacgatt ccaggtccat ccggctgccg ggtccaggcg ctcgccgtgc
     3661 ggtccgatgt gttggaagcg cgagcagtcg atgcgccata catcgccatt cttcggtggg
     3721 agagcccgtc cgtccgccag ccacttgagc cctttccatg gcatcgcaat ctccgccgtc
     3781 cagccttcat cgacatcatc gcgcttgttt agagtgccgt tgatgtggac agcgtggcgc
     3841 agaccgggaa aatcccagtc gagatatccg tatcgctcgc cacgtggatg cacatggccg
     3901 ccgaccccgg agagcgtcgc cacgttcctc cccttcgggt cccattccgg cacgttgtac
     3961 gggctgcccg gacggaacat gtctttccag atccagaaga cctcgtagat ggtattgaag
     4021 gcattgattt cgaactcgta atacgcatcc ttgcccgcg
//
LOCUS       DASTMK010000471         6451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_120193, whole genome shotgun sequence.
ACCESSION   DASTMK010000471 DASTMK010000000
VERSION     DASTMK010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120193"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFQ79_19765"
     CDS             complement(<1..785)
                     /locus_tag="VFQ79_19765"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEU0141970.1"
                     /translation="MSTLLPLDQIRTDGGTQPRAVIDFETVDDYMDAMSEGVKFPAVD
                     VFFDGTNYWLADGFHRVKAADQAGLDTIVCNVHQGTQDDAQWYSFGANKANGLRRTND
                     DKQRAVKAALLHPKSKEFSDRKIARHVGVHFNTVSGYRKQSTITKCDSQATLRQGRDG
                     RAIDVSKIGKRRTKPAPPARPMNAPAPAAEPGKATPTSEPDSGDRLASEWIAHLCHAC
                     AEIAGCPITAKELAALIRRGNSSKEILKNLESTDEFIRSVLAEA"
     gene            complement(791..1498)
                     /locus_tag="VFQ79_19770"
     CDS             complement(791..1498)
                     /locus_tag="VFQ79_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141971.1"
                     /translation="MSTQLVKFTAPKALVVAPEAADAVKEAFAVNIAGGSVSEFDLPR
                     IKVMSGAALWLIPTLEGEETAQRVEGVIAFYRDTRTYYPSKEAGNVPPQCSSADGLTG
                     RGTPGGECVRCPHAKWDTAEEGSGQACKQGKQLFFLRGDSMFPEVVSLPPTSVKNARQ
                     FFLKLTTQGVPYFQALVALELEKAQNPQGKTYGKAVIKFLRRLTPEEAARAADYHQMC
                     QQFSGRVPTEGVPDKQE"
     gene            complement(1510..1953)
                     /locus_tag="VFQ79_19775"
     CDS             complement(1510..1953)
                     /locus_tag="VFQ79_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141972.1"
                     /translation="MNLEQLKEFVCLENRKKELDAEQKQVKQQLDDLEETLVPQFIQD
                     GIQKMTVDGRTVSLVQDIYASPMYDRGDVVSALKQSELGQYVAENYNTNSLTAFVREV
                     KREVEAQAQQEQRPYDEDDIRAALPAPLGQALKISFVHRLSSRKA"
     gene            complement(1976..3646)
                     /locus_tag="VFQ79_19780"
     CDS             complement(1976..3646)
                     /locus_tag="VFQ79_19780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0141973.1"
                     /translation="MPDLVAVTQADVDAGRIFLRAPLSAFLTCRRIPNGSWDSRRKAW
                     SFPATQEQARRVQSAINRLATTPRFDELVARPEPAPAPTPAVPLASSPAPETETPEGL
                     LTRPWRHQSAAFLFCLQHFAMGLFGLLLAMGMGTGKTLVAFMVMLHRKAQRTLILCPL
                     RVIPVWVTQLERHVGIPLVIAALDDNISSVRKKQELALEKLRLAEARGVPFVCIINYD
                     SFWREPFNPWAEKLRWDLLIMDEAHRIKAPGGKASLACKRMRMRSLVRLGMTGTPMPH
                     GPLDAYAVTRPLDQTIFGPSFAAFRQKHAVLGGFQNKQVIGFQKLDELEKALARIMFR
                     VGKEVLDLPPETHVTYHCDLLGEAARVYRDLEEDFVAQVRNGVVTAANAMVKLLRLQQ
                     VTGGCVPTDDGVGHRIDSSKQKLLADTLEDIGPEEPVIVFCRFHADLDAIHEACRSLG
                     YTSLELSGRRDELAQWQAGESQVLAVQISAGGVGVDLTRARYSIYYSLSFSLGEYDQS
                     LSRVHRPGQTRPVEHIHLVARNTVDSKIMRALEKRAEVVQAILAEIKN"
     gene            complement(3639..3866)
                     /locus_tag="VFQ79_19785"
     CDS             complement(3639..3866)
                     /locus_tag="VFQ79_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141974.1"
                     /translation="MEQPNDKRKPNRQGNPLYGLNRRVARKLGKSESMVSQVRRGIAV
                     SSKVAAAIEDEARLMLMECAQSRRAQGGSRA"
     gene            3971..4345
                     /locus_tag="VFQ79_19790"
     CDS             3971..4345
                     /locus_tag="VFQ79_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141975.1"
                     /translation="MEQNQIKFGIELPKELHRQVKIAVATRGLKMKDASEQAFTAWLE
                     AVGEPGKHAHRAAGSTQVEAASPFGDLTPEEEKYVDWLLALVRQKKPTPSTEIALQAV
                     RLAGSEVVGEKSVTSRKKQKAG"
     gene            4499..4711
                     /locus_tag="VFQ79_19795"
     CDS             4499..4711
                     /locus_tag="VFQ79_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141976.1"
                     /translation="MALDFVTDADLAEVVGWQDMQWKANLEAARKIESIENRIKNGAQ
                     IVAKNYYFDHKRHLVRTKKNAAASGE"
     gene            4973..>6451
                     /locus_tag="VFQ79_19800"
     CDS             4973..>6451
                     /locus_tag="VFQ79_19800"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU0141977.1"
                     /translation="MSDQQISAPGQSAPAIRAAEYARMSTEHQQYSIENQSEAIRQYA
                     QRRGMAIVRTYADSGKSGLRIDGRDALKQLIRDVESGNADFKAILVYDVSRWGRFQDA
                     DESAYYEYICRRAGIAVHYCAEQFDNDGSPVATIVKGVKRAMAGEYSRELSAKVFAGQ
                     CRLIELGYRQGGTPGFGLRRMLLDQAGNPKGPLAHGEQKSLQTDRVILVPGPPEEVAA
                     VSRIYRMFVDESKVESEIARILNAEGVRSDFGREWSHGTIHQILTNPKYVGDNVYNRV
                     SFKLKKKRVVNPPEMWIRRDRAYEPIVEPRLYYVAQGIIRERNRRFSDAELLERLKHL
                     LEKEGRLSGFLIDEAEGMPSSSAYRSRFKSLVRAYHLIGYTPQRDLAFIETNQRLRQL
                     YPQVVADVTAKIEALGGRIERNAETGILTVNSEFTTSLVIARCRFTAAGSLRWLIRLD
                     SGLQPDITVAIRMNTANSDPMDYYLLPSIDLDSGRIRLAEENR"
BASE COUNT         1238 a         1938 c         2016 g         1259 t
ORIGIN      
        1 gcttcggcaa gaactgagcg tatgaattca tcggtgctct ccaggttttt caaaatctct
       61 ttgctgctat tgccgcggcg gatgagggcc gcgagttcct tcgcggtaat ggggcatccc
      121 gcgatttccg cgcacgcatg gcaaaggtgc gcgatccact ccgacgcgag gcggtcgccg
      181 gaatcgggtt cggaagttgg tgtggctttg ccgggctccg cggcgggagc cggcgcgttc
      241 atgggccttg caggcggcgc cggtttggtg cggcgcttgc cgattttcga tacgtcgatg
      301 gcgcggccgt cccggccctg ccgcaaggtc gcctgactat cacattttgt gatagtcgac
      361 tgtttgcggt acccggagac ggtgttgaag tggacgccga cgtgacgcgc gattttccga
      421 tcgctgaact ccttcgactt cgggtgcagg agagcggctt tcaccgctct ctgcttgtca
      481 tcgttggtgc ggcgaaggcc gttggcctta ttcgcgccga aactgtacca ctgggcgtcg
      541 tcctgggttc cctgatggac gttgcagacg atggtgtcga gtcctgcctg gtcagcggct
      601 ttgaccctgt gaaatccatc ggccaaccag tagttcgtgc cgtcaaagaa aacgtcgacg
      661 gcggggaact tcacgccctc cgacatggcg tccatgtagt cgtccacggt ttcgaagtcg
      721 atcaccgccc gcggctgcgt gccgccgtcg gtgcggatct ggtcgagtgg aaggagggtg
      781 ctcacggcgg ctactcctgc ttgtccggga cgccttcagt aggcacgcgc ccggagaact
      841 gctggcacat ctggtggtag tcggcggccc gagcagcctc ctccggggtc aggcggcgta
      901 ggaacttaat gaccgctttg ccgtacgtct tgccttgtgg gttctgcgct ttctccagtt
      961 cgagcgccac caaggcctgg aagtacggga cgccctgggt cgtgagcttc agaaagaact
     1021 gccgcgcgtt cttcacgctg gtgggcggca aggacacgac ctcgggaaac atcgaatcgc
     1081 cgcgcaggaa gaagagctgc ttcccctgct tgcaggcctg ccccgagcct tcttccgcgg
     1141 tgtcccactt ggcgtgcggg cagcggacgc actcgccgcc gggagttccg cgcccggtca
     1201 ggccgtcggc cgacgagcac tgcgggggaa cgttgccggc ctctttgctc gggtagtagg
     1261 tgcgggtgtc gcggtagaag gcgatcacgc cctcgacccg ctgcgccgtt tcctctcctt
     1321 cgagcgtagg gatcaaccag agggcggcgc cgctcattac cttgatgcgg ggcagatcga
     1381 actccgacac gctgccgccg gcgatgttga cggcgaacgc ctccttgacg gcatcggcgg
     1441 cctcgggcgc aacgacgagc gccttgggcg cggtgaactt cacgagttgt gtggacatag
     1501 ctgttccttt caagcctttc tgctgctgag tctgtgaacg aacgaaatct tcagggcctg
     1561 gccaagaggc gcgggaagag ccgcgcgaat atcgtcctcg tcgtaaggcc gctgttcctg
     1621 ctgcgcctgg gcctcaacct cgcgcttcac ttcccgcacg aaggcggtca ggctgttggt
     1681 gttgtagttc tccgcgacgt actggccgag ttccgactgc ttgagcgcgg ataccacatc
     1741 cccgcggtcg tacatcgggc tggcgtaaat gtcctggacg agggagacgg tgcggccgtc
     1801 caccgtcatc ttctggatgc cgtcctggat gaactgcggg accagggttt cttcgagatc
     1861 gtccagttgc tgcttgacct gcttctgttc ggcgtcgagt tccttcttcc tgttctccag
     1921 gcacacgaac tcctttaatt gctccaggtt cactgtgagc gtcctccttt atgggttagt
     1981 tcttgatttc cgcgaggatg gcctgcacta cctcggcgcg cttttcgagg gcgcgcatga
     2041 ttttcgagtc caccgtgttc ctcgcgacca ggtggatgtg ttcgaccggc cgcgtctgtc
     2101 cgggcctgtg gacccgtgaa agcgactggt catactcgcc gagcgagaac gagagcgaat
     2161 agtagatcga ataccgggcg cgggtgagat cgacgccgac accaccggcg ctaatctgca
     2221 ccgccagaac ctgcgattcc ccagcctgcc attgagcgag ctcatcacgg cgccctgaca
     2281 gttccaaaga tgtgtaccca agcgatcggc acgcctcgtg gattgcatcc aaatccgcgt
     2341 ggaagcgaca gaacactatg acgggttctt ccgggccgat atcctccaga gtgtcggcca
     2401 gcagcttctg cttcgatgag tcgatgcggt gacccacgcc gtcgtcggtg ggaacgcagc
     2461 cgcccgttac ctgctgcaat cgcagaagct tcaccatcgc gttcgcggcc gtgactacac
     2521 cgtttcggac ctgggcaacg aaatcctcct ccagatcccg atagaccctc gcggcttcgc
     2581 cgagcaaatc gcagtgatac gtcacgtgcg tctcaggcgg cagatccaga acctctttcc
     2641 caacccggaa catgatcctg gcgagcgcct tctcgagctc atccagtttt tgaaatccga
     2701 tgacctgctt gttctgaaag ccgccgagga cggcatgctt ctgccggaac gccgcgaacg
     2761 atggcccaaa tatcgtttgg tccagggggc gcgtcacggc ataggcgtct aggggaccgt
     2821 ggggcatagg cgtgcccgtc atccccaagc ggacgaggga gcgcatgcgc attcgcttgc
     2881 atgccaggga cgctttgcca cccggcgctt tgatgcggtg ggcctcgtcc atgataagca
     2941 ggtcccagcg aagcttctct gcccacgggt tgaagggctc gcgccagaac gaatcgtagt
     3001 tgatgatgca gacgaaaggc acgccgcgag cttctgccag gcgcaacttc tccagagcga
     3061 gttcctgttt tttgcggacg ctgctgatgt tgtcgtcgag agcggcgatc acgagcggta
     3121 tcccgacgtg ccgttccagt tgcgtgaccc aaaccgggat aacgcgcaac gggcaaagga
     3181 tcagggtgcg ctgggccttc ctgtggagca tcaccatgaa agcgacgagc gtcttgccgg
     3241 ttcccatccc catggcgagc agcagcccga aaagacccat cgcgaagtgc tggaggcaga
     3301 ataagaacgc cgccgactgg tgccgccacg ggcgagtcag gaggccttcg ggcgtctcgg
     3361 tctccggcgc tggactcgat gcaagcggca cagccggcgt cggtgcgggg gcaggctcgg
     3421 gccgagcgac aagctcatcg aaccgcggcg tggtcgccag ccggttgatg gcgctctgaa
     3481 cgcgccgggc ctgctcctgc gtggcgggaa atgaccacgc cttgcgccgg gaatcccagg
     3541 atccgttcgg gatgcgccgg caggtcagaa atgcggagag cggcgcccgc aggaagatgc
     3601 ggccggcatc cacgtcagcc tgggtcacag ccacgagatc aggcacgaga gcccccctgt
     3661 gcgcggcggc tctgggcgca ttccatcagc atcagccggg cttcatcctc aatggcggcg
     3721 gccaccttgg aggaaacggc aatgccccga cgaacctggc tgaccatcga ttcgctcttg
     3781 cccagcttgc gggccacgcg ccggttgagc ccatacaaag ggttgccttg ccggttcggc
     3841 ttgcgtttgt cgttgggctg ttccatagtt gagttgaagt tgaaatatta gtattcgcga
     3901 aatcgcgatt gttgtcaatc gtgatttatg tatagtgcgc cgaaaccggg atgaatccca
     3961 gtgcaaccct atggaacaga accaaataaa attcggcata gaactgccga aagaactcca
     4021 ccggcaggtc aagatcgcag tcgcgactcg cgggctgaag atgaaagatg cttccgagca
     4081 agcatttacg gcctggctgg aggcggtggg cgagcccggc aaacacgcgc atagggccgc
     4141 tggatcgacg caagtcgaag cagcatcgcc atttggcgac ctcacccctg aggaggagaa
     4201 gtacgtcgat tggctgctcg cactcgtgcg gcagaagaag cccactccat ctacagaaat
     4261 cgcgttgcag gcagtccggc tggccggctc tgaagtggtt ggagagaagt cagttacatc
     4321 ccggaaaaaa cagaaggccg gctgattgta gtgtgtcgca ccgcaagatg tagcggtgcc
     4381 gcagtgcccc gaacgctact aatgtgcatt tgcgttcgct ttttattcgc cctaaaattt
     4441 ctgtatggcg aacgaggcta caccgaagct tcggcttatc aagggtcggc cgaagcagat
     4501 ggcactggac ttcgtgaccg atgcggatct cgccgaggtc gtgggttggc aggacatgca
     4561 gtggaaggcg aatctggaag ctgccaggaa gatcgagtcc atcgaaaacc ggatcaagaa
     4621 cggcgcgcag atcgtcgcca agaactacta cttcgatcat aagcgccacc tggtgcggac
     4681 caagaagaac gccgccgcgt ccggtgagtg acggcgtttc ccacctcggg cctgcgaggg
     4741 gagcattcct ggggtccttc agtctgcgaa tgggggaccc acatttcctt cctgtggcga
     4801 tcgggtcaag gaatttcccg ctgacgaagg ctaccgttcc gataatcact tacgtctaat
     4861 tacttctata acttgcattc cgcgagttga agacccgtgt gtatgaggtt ccggccgttg
     4921 ccgctttgaa gggcggctcg gttccgaaag atgccttgca gatgcgaccg tcatgagcga
     4981 ccagcagatc agcgcccccg gccaatcggc gcctgccatc cgcgccgcgg agtacgcgcg
     5041 gatgtccacc gaacaccaac agtactccat cgagaaccaa agcgaggcta tccggcaata
     5101 tgcccagcgc cgcggcatgg cgatcgtccg gacctacgcc gactccggaa agagtggcct
     5161 ccggattgac ggccgcgacg cactgaagca gttgatccgg gacgtggaaa gcggcaacgc
     5221 cgatttcaaa gccatcctcg tctacgacgt cagccggtgg ggccgatttc aggacgccga
     5281 cgagagcgcc tactacgagt acatctgccg gcgcgcgggc atcgctgtcc attactgcgc
     5341 cgagcagttc gacaacgacg gtagcccggt ggcgaccatc gtcaaagggg tgaagcgggc
     5401 gatggccggc gagtacagcc gcgaactctc agccaaggtg ttcgccggcc agtgccgtct
     5461 gatcgaactc gggtaccggc agggaggcac gcccggtttc ggactgcgca gaatgctctt
     5521 ggaccaggcc ggcaacccca aaggcccgct ggcgcacggc gaacagaaga gcctccagac
     5581 agaccgcgtc atcctggtcc ccggacctcc cgaggaggtg gcggcagtaa gtcgaattta
     5641 ccggatgttc gtggatgaat cgaaggtgga atccgagatt gcccggattc tgaacgccga
     5701 aggcgtccgc agcgacttcg gccgcgagtg gagtcacggg accatccacc agatcctcac
     5761 caatcccaag tacgttggcg acaacgtcta taaccgtgtc tcgttcaaac tgaagaagaa
     5821 gcgcgttgtg aatcccccgg aaatgtggat tcggcgcgac cgcgcgtacg agccaatcgt
     5881 cgagccgcgc ctatactacg tcgcccaggg aatcatccgc gaacgaaacc gccgtttttc
     5941 ggacgccgaa ctgctggagc ggctgaaaca tctgttggag aaggaggggc ggctttccgg
     6001 cttcctcata gacgaggccg agggcatgcc ttccagttct gcgtatcggt cacgattcaa
     6061 gagcctcgtc cgggcgtacc atctcatcgg ctacacgcca cagcgcgacc tggccttcat
     6121 cgaaaccaac cagcgcctac gccaattgta cccgcaggtt gtggccgatg tgacggcgaa
     6181 gatcgaagcg ctgggcgggc gcatcgaaag aaatgccgaa accgggatac tgaccgtcaa
     6241 cagcgagttc acgacctcgc tggtcatagc gaggtgccgg ttcacggcgg cgggctcgct
     6301 gcgttggctc atccggctgg atagtggcct gcaaccggat atcacggtgg ccatccgaat
     6361 gaacaccgcc aacagtgatc cgatggatta ctatttgctc cccagcatcg atttggattc
     6421 cggaagaatc cgccttgccg aagaaaaccg t
//
LOCUS       DASTMK010000472         8087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_408197, whole genome shotgun sequence.
ACCESSION   DASTMK010000472 DASTMK010000000
VERSION     DASTMK010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408197"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFQ79_19805"
     CDS             complement(<1..613)
                     /locus_tag="VFQ79_19805"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEU0141978.1"
                     /translation="MNEIELLPHSRVREVFGLAESAARELGIRDVEVIVAAESSALTR
                     FANNAIHQNVAQQVQTVSVRTARDNRTARATTNCLDAESIRRAVAQAAAIMDSVEPLP
                     YLPPMAGPAPFEEVDRFCTATAEASPVDRARAVTEAIGIAEGASQTAAGIFSTEQGAH
                     AILNSAGVFAYHAATMARFSMTAMTGDSSGWAKASSVDRREIDA"
     gene            complement(617..2074)
                     /locus_tag="VFQ79_19810"
     CDS             complement(617..2074)
                     /locus_tag="VFQ79_19810"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU0141979.1"
                     /translation="MKQTALEALDAAMRNGVSYADVRLIEDRNRRLSTKNGTAGDVSS
                     SESAGIGIRVIASGCWGFAATDDLTKDGIRRAAALAVEIAQASALARRHELALAPEEA
                     YRADWVSPCAIDPFAVRIEDQLQLLMAVDGTLRQTPGVTLAETSMDFTRRRQVFASSA
                     GSIIDQTRTTTGAGFTVYSFKENEIQKRSYPNSFGGQYQLKGYELVDELKLIENAPRI
                     AEEAVALHSADQCPEGVFDLILDSSQLALQIHESIGHPIELDRVLGSEANYAGMSFLT
                     LDQLGTLQYGSGIVNVVCDARLEHGPGLGTFAFDDEGVPAQRVDVIRNGLFTGYMTSR
                     ETAAAAGDARSNGTMRADGWTRIPLIRMTNVSLLPGEQSLEQVFGEVERAIYMETNRS
                     WSIDDKRYNFQFGCEIAWEIRNGKRVRMLKNPSYSGISTEFWNSCAAIAGREHWTLWG
                     VPNCGKGQPEQVMGTGHGASPARFSAVKIGGAYQE"
     gene            complement(2071..3228)
                     /locus_tag="VFQ79_19815"
     CDS             complement(2071..3228)
                     /locus_tag="VFQ79_19815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0141980.1"
                     /translation="MNSRSRPLLVAHVGFVLTGAITILLGPILPSLSARWALTDSQAG
                     ALFAVQFGASLAGTAVSGRLSARFGFVSALSAGFAVVALGLAGLTLPSWPAGVLAVLL
                     YGGGLGIVIPTANMLVSHLQPHRQAAALNILNFAWCIGAAATPPAIAFLLERLGLNAT
                     LWGLSGTVVLLSGWAAVCPGKQRPAGTHRSHAAEWRTGFSLLSGVLLFLYVGAEMSVS
                     GWIGTYILRRLDGGGGLWAFGTSVLWSAILVARLAAPAALRRISGHALVFAGACVATG
                     GLAVILTGRNAAALFTGVFLTGLGFGPIFPTVVANFTGYFGGAASHIAWFIFAMGGLG
                     GAVVPSLVGQLSTASGSLQTGLSATVLCILGVVLIEPALRRCASRAVESAL"
     gene            complement(3225..4652)
                     /locus_tag="VFQ79_19820"
     CDS             complement(3225..4652)
                     /locus_tag="VFQ79_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEU0141981.1"
                     /translation="MRRKIRDWISPIVYLSNNWISLTGVVLVTGAAVIWILLLPTMLR
                     GDAATPYIGILTFLILPVIFFTGLGLIPLGIWLRSKREHTSGIYPTAFPPVDMRNQAF
                     RRLLTFVALATFANIVIGSQLTYRAVHYMDSVTFCGQACHTVMQPEFAAYQNSPHARV
                     ACVDCHIGPGASWFVRSKLSGAGQLLAVTFNTYPRPIPTPIENLRPARETCEQCHWPD
                     KFSADRIRVIPKYAEDEANTESKTVLLMHIGGGPQVRGIHGVHLGPGVTIRYAHSDDK
                     RLEIPWVEYNDGKGNTTQYASPDFKPGAENTMRVRVMDCLDCHNRPTHVFELPETALN
                     HAIASGEISPTLPYAKKKGLELLKVSYNNHDDAQKRITEEFAAYYREQYPEIFEKQPI
                     EVKRAASAVWNIYSRNVFPEMKVTWGTYPNHIGHTDFPGCFRCHDDNHPSASGKNLTQ
                     DCSACHNLLAMDEENPEILKTLGAR"
     gene            complement(4655..6190)
                     /locus_tag="VFQ79_19825"
     CDS             complement(4655..6190)
                     /locus_tag="VFQ79_19825"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU0141982.1"
                     /translation="MTCLECHPKFTAYPHPENVPKPQCASCHPQQAGEHSRSVHGLAL
                     EAGNAAAPGCATCHGDAHELPRAGTPEFHRLVPETCGMCHTEVAEQFNASVHGQAVAR
                     GIREAPVCTDCHGEHEIRPHTDTRSTVNPLQIRETCGSCHDDVRLGRRFNIPLDRIRS
                     FDESFHGLALKAGSQTVANCASCHGVHNIFRSTDRRATTNVANLPQTCGQCHPGAGSR
                     FAIGTVHLTEGFEPAVVVWVRRFYLGVIPFTLGFMILHHLGDWIRKLLRMRLHAQVGA
                     PAPVPLRHAEVRMLRFERIQHFLLLTSFMVLVWSGFALKYPDAWWAMPLVAYESAYPF
                     RGTIHRIAAAVLVAVSIMHVISLLVNRRLREHWLTMLPRRNDVYEMLGALAYNLGLRS
                     TKPVISPHSYVEKVEYWAVVWGTFIMAATGFLLWFNNWSLRWMPKSWLDAATSIHFYE
                     AVLASLAILIWHFYTVIFDPDVYPMDPAWLTGRSVRRRVSHSPEENAVETDSSEKQQS
                     SAD"
     gene            complement(6386..6670)
                     /locus_tag="VFQ79_19830"
     CDS             complement(6386..6670)
                     /locus_tag="VFQ79_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141983.1"
                     /translation="MNLIPYVVLWAVMASGVIALLVWRKLVSSKEDDVLHVLEGESAA
                     IPAQQEIARKLSTIDRWGKVLTAIAFVFGVALAAAYVYQVWERSNQIVIR"
     gene            complement(6732..7154)
                     /locus_tag="VFQ79_19835"
     CDS             complement(6732..7154)
                     /locus_tag="VFQ79_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141984.1"
                     /translation="METVQLAITDVPYANALREILVRNGAFEVVCVDRPDSVLDGVLV
                     LDADHLDLLPVPLANPERVVLIARNDPALLTRAWEAGVNSVIFEKDPLNTVVLAIMSA
                     RLRLPRSKRLMAGGLGMLKDSGTGEKQASDSPPGKGNL"
     gene            complement(7182..7391)
                     /locus_tag="VFQ79_19840"
     CDS             complement(7182..7391)
                     /locus_tag="VFQ79_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141985.1"
                     /translation="MRVFKFLIPGFVFIFGLALFTPGTYGKPEDAKKTGEKCVTCHPP
                     GKMKELTEAGKYYKEKKTLDGYKKK"
     gene            7633..>8087
                     /locus_tag="VFQ79_19845"
     CDS             7633..>8087
                     /locus_tag="VFQ79_19845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0141986.1"
                     /translation="MPKLMERWQRANVLGAVFDQLSDALVLYDANLTISGVNQAAERL
                     FGMTAEEMVGRHCNDVFRCAVCEPGCGMLVGLNQAPNGAQSTVRLHTDNGRERLALIR
                     TNQLLDENGNVDGAVATIKDITEEVAPQKREIIAESASMRDVMHFVRRVA"
BASE COUNT         1668 a         2474 c         2374 g         1571 t
ORIGIN      
        1 ctgcatcgat ctcccgccgg tccactgaag acgccttcgc ccacccggag ctgtcgcccg
       61 tcatagcggt catcgaaaac cgggccattg tagcggcatg ataggcgaac acgccggccg
      121 agttgaggat ggcgtgcgcg ccctgttcag ttgaaaagat tcccgccgcg gtttgcgatg
      181 caccctccgc gatgccgatc gcctcggtga cggcacgcgc gcggtcaacg ggactggctt
      241 cggccgttgc cgtgcaaaac cggtccacct cctcgaacgg cgccggtccg gccatcggcg
      301 gcaggtacgg cagcggttcc accgagtcca taatggccgc agcctgcgca acggcgcgcc
      361 gaatcgactc cgcgtccaaa cagttggtgg tggcgcgcgc ggtacggttg tcgcgcgccg
      421 ttcttaccga gacggtctgc acctgttggg ctacgttctg gtgaatggcg ttgttcgcga
      481 agcgggtcag cgcgctcgat tccgccgcga caatcacctc gacatcgcga atgcccagct
      541 cacgcgcggc gctttcggca agaccgaata cttctcttac acggctgtgt ggcagcagtt
      601 caatttcgtt cacgggttac tcctggtagg ccccaccgat cttcaccgcg ctgaagcgcg
      661 ccggactggc tccgtgaccc gttcccatca cctgctccgg ctgccctttg ccgcagttcg
      721 gcacacccca cagggtccaa tgctcgcgcc ccgcaatggc cgcgcacgag ttccagaact
      781 ccgtgctgat tccgctgtag ctcgggtttt tgagcatgcg gacacgtttg ccattccgga
      841 tttcccatgc gatttcgcat ccaaactgaa agttgtaccg cttatcgtcg atagaccagc
      901 ttcggttggt ctccatgtag atcgcacgct ccacctcgcc gaacacctgc tcaagggatt
      961 gctctcccgg caacaggctc acgttggtca tgcgtattag cgggatgcgc gtccatccgt
     1021 cggcccgcat ggtgccgttc gacctggcgt caccggcagc cgccgctgtc tcacgcgaag
     1081 tcatgtagcc ggtgaagagg ccgttgcgaa tgacgtccac acgctgcgcg ggaactcctt
     1141 catcgtcgaa ggcgaacgtt cctaaaccgg ggccgtgttc caggcgcgcg tcacaaacca
     1201 cattcacgat accggaaccg tactgaaggg tcccaagttg atcgagcgtc aggaaactca
     1261 tgcccgcgta attcgcttcg ctgccgagca cacggtccag ctcgatagga tggccgatcg
     1321 attcgtgtat ctgtagcgca agctggctgc tgtccaggat cagatcgaag acaccttccg
     1381 ggcattgatc cgccgagtgg agcgcgacgg cctcttccgc gatgcggggc gcgttttcga
     1441 tcagcttcag ttcgtcaacg agttcgtaac ctttcaactg gtactggccg ccaaacgagt
     1501 tcggatagga acgcttttgg atttcattct ctttgaagct gtagacggtg aaccccgcgc
     1561 cggtggtggt acgcgtctgg tcgattatgc tcccggccga cgatgcgaat acctgccgcc
     1621 ggcgtgtgaa atccatcgac gtctctgcca gcgtcacgcc gggagtctgg cgcaaggtgc
     1681 catcgacggc catcagaagc tgtaattgat cctcaatccg cacggcaaac ggatcgatgg
     1741 cgcaaggaga aacccaatcg gcgcggtatg cctcctccgg cgccaacgct aactcgtgcc
     1801 ggcgggccag ggcgctcgct tgcgcaatct cgaccgcaag cgctgccgct ctccggatgc
     1861 cgtcttttgt caggtcgtct gtggccgcga agccccagca ccccgacgcg atcacgcgaa
     1921 ttccaatgcc cgcggactcc gagctcgata catcgccggc tgtgccgttc ttcgtcgaaa
     1981 ggcggcgatt cctgtcttcg atgaggcgaa cgtcggcgta ggagacaccg tttcgcatgg
     2041 ctgcatccag cgcttcgagc gcagtctgtt tcatagagcc gattctacag cccgcgatgc
     2101 gcatcttcga agagcgggtt caatgaggac cacgccgagg atgcacaaca cagtcgccga
     2161 cagccctgtc tggaggctgc ccgaagcggt ggatagctgc ccgacgaggg aaggaaccac
     2221 cgcgccgccc aatcctccca tggcgaaaat gaaccaggcg atgtgcgatg ccgcgccccc
     2281 gaaatagccg gtgaagttcg ctaccacggt cggaaagatg gggccgaagc cgagaccggt
     2341 cagaaacact ccggtgaaca gtgcggcggc gttcctaccg gtgaggatta ctgccagccc
     2401 gcccgttgcg acgcatgcgc cggcgaaaac cagagcatgg cccgagatgc gtcgaagagc
     2461 ggcgggggcg gcaaggcggg ccaccaggat cgcgctccag agcacggatg tgccgaaggc
     2521 ccataggccc cctcctccat cgagccgccg caggatgtag gttccgatcc agccgctcac
     2581 ggacatctct gcgcctacat aaaggaacaa gagcactccc gagagcagag agaatccggt
     2641 gcgccattcg gccgcgtggc tgcgatgcgt ccccgccggc ctttgtttgc cgggacagac
     2701 cgcggcccac ccgctgagca ggaccacggt tcccgacaac ccccacagcg tcgcattcag
     2761 gcccaggcgt tccaacagaa aggcgatggc cggaggtgtg gccgcggctc cgatgcacca
     2821 cgcgaaattc agtatgttga gcgccgcagc ctgccggtgc ggctggagat gcgagacaag
     2881 catgttggcg gtgggaatga caattcccag gccgccgcca tagaggagca ccgccagaac
     2941 cccggcgggc caggaaggca gagtgagtcc cgccaatccc agcgcgacca ccgcgaaccc
     3001 ggcggagagg gcggatacaa agccgaatcg cgcgctgagg cgccctgaaa ccgctgtacc
     3061 tgccagcgaa gcgccaaact ggacagcgaa caaagctccg gcctgtgaat ccgtaagcgc
     3121 ccaccgggcc gaaaggctcg gcagaatcgg tcccaacaga atcgtaatag cgccagtgag
     3181 tacgaatcct acatgcgcaa cgagcagcgg ccgggaccgc gaattcatcg agccccgagc
     3241 gttttcagga tctcgggatt ctcttcgtcc atggccagca gattatgaca ggcgctgcag
     3301 tcctgcgtca ggttctttcc cgaagcgctg gggtgattgt cgtcgtggca ccggaaacat
     3361 cccggaaagt ccgtatggcc gatgtggttc ggataggtgc cccaggtcac tttcatttcg
     3421 gggaaaacat tccgcgagta aatgttccag acagccgaag ccgcccgttt tacctcgata
     3481 ggctgcttct caaaaatctc cggatactgc tcgcggtagt acgctgcaaa ctcctccgta
     3541 atgcgcttct gcgcatcgtc gtgattattg taggagacct tcaacaactc caaacccttc
     3601 ttcttggcgt atggaagggt gggtgagatc tcgccggaag cgatggcatg atttagcgcc
     3661 gtttcgggaa gctcgaacac gtgcgtgggc ctgttgtggc aatcgagaca atccatcaca
     3721 cgcacacgca tggtattctc cgcgccgggt ttgaaatcgg gagaagcgta ttgcgtcgtg
     3781 ttgcccttgc cgtcgttata ttccacccat ggaatttcca gccgcttgtc gtcactgtgg
     3841 gcgtatcgaa tggtgacacc cggaccgagg tgcaccccat ggatgccccg cacctgcggc
     3901 cccccgccga tgtgcatcag cagaacggtt ttgctctcgg tgttggcttc atcttcggcg
     3961 tatttcggta tgacccgaat gcggtccgcg ctgaacttgt cgggccagtg gcactgctcg
     4021 cacgtctccc gggcagggcg cagattctcg atcggcgtcg gtatcgggcg cgggtaggta
     4081 ttgaacgtga ccgccaatag ctgccccgca ccggaaagtt tgctgcgcac aaaccaggaa
     4141 gcgcccggac cgatgtggca atccacacac gccactctgg catgcggcga gttctggtag
     4201 gccgcaaact cgggctgcat caccgtatgg cacgcctgtc cgcaaaaggt cacactatcc
     4261 atgtagtgaa cggcccggta ggtgagttgg gaccctatga cgatattcgc gaaggtagcc
     4321 agcgcgacaa acgtaagaag ccgtctgaac gcctggttgc gcatatcgac aggcggaaat
     4381 gccgtgggat agatgccgga tgtgtgctca cgcttcgacc gcagccagat gccgagcgga
     4441 atcaggccga gacccgtaaa gaaaatcacg ggaagtatga ggaacgtcag gatgccgata
     4501 tacggcgttg ccgcgtctcc cctcagcatg gtcggcagta gcaggatcca gatgaccgcc
     4561 gctcctgtta ccagcacgac acccgtaagg ctgatccagt tgttggaaag atagactatc
     4621 ggggaaatcc aatctcggat ttttcttcgc atgttcagtc cgctgagctt tgttgttttt
     4681 cggagctatc cgtctcgacg gcattttcct ccgggctgtg agaaacacgg cgccggacgg
     4741 aacggcccgt cagccaggca ggatccatcg gatatacatc cggatcgaag atcacggtgt
     4801 agaagtgcca gatcagaata gcgagcgagg cgagcaccgc ctcgtagaaa tggatggagg
     4861 tggccgcgtc gagccacgat ttcggcatcc accttagaga ccagttattg aaccagagca
     4921 ggaagcccgt cgccgccatg atgaaagtgc cccagaccac cgcccagtat tcgacttttt
     4981 cgacgtagct gtggggagag atcaccggct tcgtggaacg caggccgagg ttatacgcca
     5041 gagcccccag catttcgtag acatcgttgc gccggggcag catcgtcagc cagtgttcgc
     5101 gcaagcggcg attcaccagc agcgagatga cgtgcatgat ggaaacagcg acaagcaccg
     5161 cggcggcaat gcggtgaatg gtgccacgaa acggatacgc gctttcatag gcaaccaggg
     5221 gcatggccca ccacgcatcg ggatacttca gggcgaagcc gctccatacc agcaccatga
     5281 aagaggtgag cagcagaaaa tgctgaattc gctcgaaccg cagcatgcgg acttcggcat
     5341 ggcgcaaggg caccggcgcg ggagcgccca cttgcgcgtg cagccgcatg cgcaacagct
     5401 tcctgatcca gtcgcccaaa tggtggagaa tcatgaagcc gagcgtgaat ggaattacgc
     5461 ccaggtagaa ccgccgcacc cacaccacaa ccgccggctc gaagccttcg gtgaggtgta
     5521 cggtgccgat ggcgaaacgg gaaccggcgc cgggatggca ctgtccgcag gtctgaggta
     5581 ggttggcaac gttcgtcgtg gcgcgtcgat cggtggaacg gaagatgttg tgaacgccgt
     5641 ggcaactggc gcagttcgcc actgtctggg aacccgcctt caatgccagt ccgtggaacg
     5701 actcgtcgaa gctccggatg cggtccaggg gaatattgaa gcggcgcccg agccggacgt
     5761 cgtcgtgaca actcccgcag gtctcacgga tctgcaatgg gtttaccgtc gaacgcgtgt
     5821 cggtgtgcgg acgaatttcg tgctcgccgt ggcagtcggt acaaaccgga gcttcacgga
     5881 tccctcgcgc cacggcctgc ccgtgaacgc tggcgttaaa ctgctcggcc acttcggtgt
     5941 ggcacatgcc gcaggtttcc ggcaccaggc ggtggaattc gggcgtaccc gcgcgaggca
     6001 gttcgtgcgc gtctccatga caggtagcgc atcctggcgc ggccgcgttg ccggcttcta
     6061 acgccagtcc gtgaacgctt ctcgaatgct cgcccgcctg ttgcggatgg cacgaagcgc
     6121 actgcggctt gggcacgttc tccggatggg gataggcagt gaacttggga tgacactcga
     6181 ggcaggtcac cttagcatgc gcggactgcg gcagcttggc tccctgatcg tggcaagagg
     6241 agcaatccgt ttcctcttgc cccaagccgg gcgcgggcga taacagacaa aacagtaaaa
     6301 gaaagaaaaa agagccgttc ggcaaaatcc tcatcgattc tcgtccgccc ggcccggccg
     6361 gggcatggga aggctacttc ggcgtctatc ttattacaat ttggttggac cgttcccaaa
     6421 cctggtacac ataggcagca gccagcgcca ccccaaaaac gaaagcgata gcggtcagca
     6481 ccttacccca ccggtcaatg gtgctcagtt tcctggctat ttcctgctgt gcgggaatgg
     6541 ccgcgctctc tccttccagc acatgcaaca cgtcatcttc tttgctcgat acgagcttgc
     6601 gccacaccaa aagtgcaatg actccactcg ccatcacggc ccatagaaca acgtatggta
     6661 tcagattcat tgccatctcc tttctagcct gcaaacgaac gcctgttcat gaactaggcg
     6721 gtatttcaga gttacaggtt accttttccg ggaggagaat cgctggcctg cttctctcct
     6781 gttccactgt ccttcaacat tcccaggcct ccggccatca gccgtttcga cctcggaagc
     6841 ctcaaccgcg cggacataat ggcaaggacc actgtgttca gcgggtcctt ctcgaaaatg
     6901 accgagttca cccctgcctc ccaggccctg gtcagcaatg cgggatcgtt tcgggcgatc
     6961 agcacaaccc gttccggatt tgccaggggc accggaagca gatccaggtg gtccgcatcc
     7021 aacaccaaca ctccatcgag aacggaatcc ggacggtcca cgcaaaccac ttcaaatgcg
     7081 ccgttccgca ccaggatctc tcgtagcgcg ttcgcgtacg gaacgtcggt gatggctagt
     7141 tgaacggttt ccatactgct ttccaggccc tgtccggctg gctacttctt cttgtacccg
     7201 tcgagcgtct ttttctcctt gtagtacttg cccgcttccg ttaactcctt catctttccc
     7261 ggaggatggc acgttacgca tttctcgccc gtcttcttcg cgtcctccgg cttgccataa
     7321 gtacccggtg tgaaaagtgc gagcccaaat ataaatacaa aacctggaat gaggaattta
     7381 aatactctca tgtagctctc cgattcccct tgatgcaatc acgtagccaa caaccgatgc
     7441 cacacaaatt caatagcttg aagaagactc tgagctgaaa ctggtagtac tgagaggccc
     7501 gcgccggttt cgcgggccaa ttgccccaac cgccgcgatg ggcgttgagc gatggtgggc
     7561 taaatggcgc agatccggca ctttccccgc atgggaactg atatactgga gttaaaaggc
     7621 ctaacgacct aaatgcccaa actgatggag agatggcaac gggccaatgt gctcggagcc
     7681 gtcttcgatc aactctccga tgctctcgtg ctttatgacg ccaacctgac gatttccggc
     7741 gtgaaccagg cagcggagcg tttgtttggc atgacggcgg aagagatggt tggccgccac
     7801 tgcaacgacg tattccgctg cgcggtgtgt gagcccggct gcggcatgct cgtgggtttg
     7861 aaccaggctc ccaacggcgc gcaaagtacg gtcaggcttc ataccgacaa cggacgcgaa
     7921 cggctcgcac tgattcgtac caatcaattg cttgatgaaa atggcaacgt cgacggtgcg
     7981 gttgccacga tcaaagacat taccgaagaa gttgcgcctc agaagcgtga gatcattgcg
     8041 gagtcggcca gcatgcgcga tgtcatgcac ttcgtgcgcc gcgtggc
//
LOCUS       DASTMK010000473         5272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_408204, whole genome shotgun sequence.
ACCESSION   DASTMK010000473 DASTMK010000000
VERSION     DASTMK010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408204"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFQ79_19850"
     CDS             <1..973
                     /locus_tag="VFQ79_19850"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU0141987.1"
                     /translation="AYMQLSRGESIALGRKTTSIRQWAERLAERGRSSTLRDELEFWL
                     HAQSDFSPLPLDHPSGENTVASTREVVVSLGREETRALLHEVPAAYRTQINDALLAAF
                     VDSFRRWTGRPSLLLDLEGHGRDATLENVDVSRTVGWFTTIFPVLLNAGAMENPGELL
                     KSIKEQLRRVPNRGIGYGLLRYAAGDEEIVARLRSLPQPEVIFNYLGQFAADPGDSHT
                     AAPADAMGPVRSPRGRRRHRIEIVGSIASERLQLSWFYSENLHRRETIERLAEQFMEA
                     SRAIVAHCRTAEAGGFTPSDFARARVSQKDLDKLVGRLRQGGARKPV"
     gene            970..4092
                     /locus_tag="VFQ79_19855"
     CDS             970..4092
                     /locus_tag="VFQ79_19855"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU0141988.1"
                     /translation="MSAAAQSNAIEDIYEFSPLQQGMLFHCLYNPKTWMYFEQVVVPV
                     RNRVNVACLTRAWQRVLERHPALRTSFHWEGLDKPLQVVHREVEFSIEQHDWSGVDAD
                     GRECRLEDFLKADRDRGFVLSSAPLIRLTLVQFSPEDYRYVLSFHHLLMDGWSLQVVN
                     HEVSVLYEAYCLGREMQLEASRPYGDYIAWLQGQDANAGKTFWRGTLSGFEGPAPLGV
                     ERPPGADGTEEIYSEQEMRLPREATAALQSLAQRTHVTLNTVMLGAWALLLGRYSGEK
                     DVVFGSVVSGRPPELRGVESMVGLFINTLPVRTQLDEEAPLSGWLRRLQEHHLEARRY
                     EFTSLTDIHTWSGRPAGMPLFQSVLAFENYPTGAMSTPQAENFEGFRQFERTNYPLSA
                     VVVPGAELLVRLLYDLHRFEHSTITRLLGHLETILVSMGGDAEQRLGDVSVLPPAEVR
                     QLMVEWNNTKVEPAGTQCVHRLFEAQVERTPDAPALAFGGQRVTYRSLNAQANQLARS
                     LQALGLRAGSLAGISMERSPQWIAAVLAVWKAGAAYVPLDPALPPERLSFMVKDAGLD
                     VLLTQEELRAALPPDVSNLEDTATPRDAAYVIYTSGSTGRPKGVIVEHRGLSNTAEAT
                     VRTLGLGPGDRVLQFAPWSFDASVYELMMTLLSGAELFLADPDSLLPGEGLLRLLQQE
                     KITTISLPPSALAAMPVREMPYLKLISVLGEACPADLAAAWAPGRRIFNAYGPTETTV
                     WVAGAFIDGSRKPPIGRPIVNTRIYLLDSRLRPVPIGVPGEICVGGMGVARGYLNTPE
                     LTAARFIPDAFSGEAGARLYRTGDLARYLQNGELEFLGRRDQQIKLRGFRIEPGEIET
                     VLAQHATVRQAVVTVREDAPGDRRLVAYIVCDAPEPPAAELRRFLRSRLPEYMIPSAF
                     VFLESLPLLSNGKANLRALQAPVREPRPNTYVAPRTSVESKLATLWAEVLRVERAGVH
                     DNFFDSGGHSLLATQLLSRVREAFEVELPLRSLFDAPDLASLAELITSLAKAPAVAEA
                     AGGEYEQGEL"
     gene            4129..>5272
                     /locus_tag="VFQ79_19860"
     CDS             4129..>5272
                     /locus_tag="VFQ79_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141989.1"
                     /translation="MSMDGQSTAAAMREPVYNERGVIAENYYDLQGLGVLEAARRAYP
                     LPKASRESTLRSVFQSVEIALLNLHDLVTRAADDVAGGRAASACVKLFWMRGFHRLLN
                     RLSMIPQQLGIGQMESVNGGILRIADSPAFNNYCEALHRFDASVIESIDSGALDAEHA
                     VADRSLDDYEFNLLHLARVCNHESTIWERNLAEVRVPVPVASYSEFVVAEGMRNAVFD
                     RVLSGDTYFTQFRGLHQIPETLGEEINDRCEEAIRDIRTTRLRHAVEHLDCIHVLSEG
                     VLAAVPPMADQLATADYHQIRENLGLTSGSHSVCLRYHMFTHLYEQLWDEYCTCVTGK
                     TANARTDVEVEEALRALEGNCQEDTVAWTMHLLGNCCLKLRAFISAW"
BASE COUNT          982 a         1561 c         1683 g         1046 t
ORIGIN      
        1 cgcgtacatg cagctcagcc gtggagaaag catcgcgctc ggccgcaaga cgacatctat
       61 ccggcagtgg gcggagagac tggcggaacg cggccgctcg tccacgctgc gcgatgagct
      121 ggagttctgg ctccacgcac agtccgattt cagcccgctt ccgctcgacc atccaagcgg
      181 cgaaaacacc gtggcctcca ccagggaggt cgtggtttca ctcggccgcg aggagacacg
      241 cgcgctgctg cacgaggttc ccgcggccta tcgcacgcaa atcaacgatg cgctgctggc
      301 agcgtttgtg gatagtttcc gccgctggac cgggcgtcct tctctgctgc tggacctgga
      361 gggtcacggc cgcgacgcaa ccttggaaaa cgtggatgtg tcgcgcaccg taggctggtt
      421 cactacgata ttcccggtgt tgctgaacgc cggcgcgatg gagaaccccg gtgaactgtt
      481 gaaatccatc aaagagcaac ttcgccgcgt cccgaatcgc ggcatcggct atggcttgct
      541 gcgctatgcc gcgggcgatg aagagatcgt ggcccggctt cgctcactgc cgcagccgga
      601 agtgatcttc aattatctgg gacagttcgc ggcggacccg ggcgattcgc acaccgccgc
      661 tccggccgat gccatgggcc ccgtacgcag cccgcgcggg cggcggcgac accggatcga
      721 gatcgtcgga agcatcgctt cggaacgtct tcagctctcc tggttttaca gcgagaacct
      781 gcaccgccgc gagacgatcg aaagactggc ggagcagttt atggaggctt cgcgcgcaat
      841 cgtcgcccat tgccggacgg ctgaggcggg cggcttcacg ccatccgatt ttgcccgggc
      901 gcgcgtaagc cagaaagatc tggacaagct ggtcggcagg ctccggcagg gcggcgccag
      961 gaaacccgta tgagcgccgc cgcacagtcg aacgccatcg aggacatcta cgagttctct
     1021 ccgctccagc agggcatgtt gtttcattgc ctctacaatc cgaaaacctg gatgtatttc
     1081 gaacaggtcg tggtgccggt tcgcaaccgt gtcaacgtgg cctgcctcac ccgggcatgg
     1141 cagcgggttc tcgagaggca tcccgcactg cgcacctcct tccactggga gggcctggat
     1201 aagccgttac aagttgtcca ccgggaggtt gagttttcga tcgaacagca cgactggagc
     1261 ggcgtcgacg ccgacgggcg ggagtgccgc ctggaagatt tcctgaaagc cgaccgggac
     1321 cgcggctttg ttctctccag cgctccgttg atccgcctga cactcgtcca gttctccccg
     1381 gaggattacc ggtacgtact gagctttcat cacctcctga tggacggctg gagcttgcag
     1441 gtagtgaatc acgaagtctc cgttctttat gaggcttact gtctcgggcg ggagatgcag
     1501 ttggaagcga gccggcccta tggcgattac atcgcctggc tgcaagggca ggacgccaat
     1561 gcgggcaaga cgttttggcg cggaacgctg agcggttttg aaggtccggc gcccctgggt
     1621 gtcgagcggc ctcccggcgc agacgggacg gaggagatct attccgaaca ggagatgcgg
     1681 ctgccacgcg aagctacggc ggccctacaa tccctggcgc agcggactca cgtaactctt
     1741 aataccgtaa tgctcggcgc ctgggccctg ctgctgggcc gttatagcgg cgaaaaggac
     1801 gtcgtattcg gcagtgtggt ttccggccgc ccgccggagc tgcggggagt ggagtcgatg
     1861 gtcggactct tcatcaacac gcttccggtg aggacgcagc tcgatgagga agcgccgctc
     1921 tccggctggc tgcggcgatt gcaggaacac cacctggaag cgcggcgtta cgagttcacg
     1981 tcgctcacag acattcacac ctggagcggc agacccgcgg gcatgccgtt gttccagtcc
     2041 gtactggcgt tcgagaacta tcctaccggt gctatgagca caccgcaggc ggagaacttc
     2101 gaagggttcc gccagttcga aaggaccaac tatccccttt ccgcggtcgt cgtgcccggc
     2161 gcggagcttc tggtgcggct cctctacgat ctccaccggt tcgaacacag caccattacg
     2221 cgcctgctcg gacatctcga aaccattctg gtcagtatgg gcggcgatgc ggaacagcgc
     2281 cttggcgacg tatctgtgct gcctccggct gaagtgcgtc agttgatggt cgaatggaac
     2341 aacacaaagg tggagccggc gggcacgcag tgtgtgcatc gcttgttcga ggcacaggtg
     2401 gagcggacac ccgatgctcc ggcgctcgct ttcggaggac agcgggtcac ttatcgaagt
     2461 ctcaacgcac aagcgaatca actggcccgc tctcttcaag cgcttggtct ccgagcaggt
     2521 tccttggccg gcatttccat ggagcgctca ccgcaatgga ttgccgccgt gctggcggta
     2581 tggaaagccg gcgcggctta cgtgccgctt gatccggcgc ttccgccgga gcgcctgtcg
     2641 ttcatggtca aagacgcggg cctggacgtg ttgcttacac aagaggaatt gcgggccgcg
     2701 cttcctccag atgtatccaa ccttgaagat acggcaacac cccgggatgc ggcgtatgtc
     2761 atatacactt ccggttccac cggcagacct aagggcgtca tcgtcgagca ccggggtctc
     2821 tccaatacgg cagaagcaac tgtgcggact ctggggttag gtcccggcga ccgggtactt
     2881 caatttgcgc cgtggagttt cgacgcttcc gtgtacgagc tgatgatgac actgctctcg
     2941 ggagcggagc tcttccttgc cgatccggat tcgcttctgc cgggtgaagg actattgcgg
     3001 ttgttgcagc aggagaagat caccaccata tctctgccgc cttcggcgct ggcagccatg
     3061 ccggtgcggg agatgccgta cctaaagttg atcagtgtac ttggagaagc ctgcccggcc
     3121 gatttggctg cggcctgggc gcctggacgc cggatcttca acgcgtacgg accgacggag
     3181 acgacggttt gggtcgccgg cgcattcatt gacgggagcc gaaagccgcc tatcgggcgc
     3241 cctatcgtga acacacggat ttatctgctg gactcgcgcc tccggccggt gcccatcggg
     3301 gtgcctggcg aaatttgcgt aggtggaatg ggtgtggcac gtggttacct gaatacaccg
     3361 gaattgactg ccgccaggtt cattccggac gcgttcagcg gcgaggcggg cgcgcgcctg
     3421 taccggacgg gcgacctggc ccgctatctc cagaatggag agctggagtt tctcggccgc
     3481 cgcgatcagc aaatcaaact tcgcgggttc cgtatcgagc cgggagagat tgaaacggtg
     3541 ctggcccagc atgcaaccgt tcgccaagcg gtcgtcaccg ttcgggaaga tgcgcccggc
     3601 gaccgccggc tggtggccta catcgtgtgc gatgcgccgg agccgccggc cgccgaatta
     3661 cgacgcttct tgcggtcacg gcttcctgag tacatgatcc cgtctgcctt cgtctttctg
     3721 gaatcgctgc ccctcctgtc aaatggcaag gcgaatctcc gggcgctgca ggcccctgtg
     3781 cgggagccgc ggccgaacac ttatgtcgcg cctcgaacca gcgtggagtc gaagctggca
     3841 acgctttggg cggaagtgct gcgagtggag cgggcgggtg ttcacgataa tttcttcgat
     3901 tcgggcggcc attcgctgct cgcaacacag ttgttgtcca gggtgcggga agcgttcgaa
     3961 gtggagcttc cgttgcgaag cctgtttgac gcgccggacc tcgcatccct ggcggaactc
     4021 atcacttcgc tcgcgaaggc gccggcggtg gccgaagccg ctggcgggga gtatgagcaa
     4081 ggtgaactgt agaggcgccg gacatccgga agcgcaagga ggtcggtcat gtccatggac
     4141 ggtcaaagta cggctgccgc aatgcgggaa cccgtttata acgagcgggg tgtcattgcc
     4201 gagaactatt acgatcttca ggggcttggc gtgctggaag cggcaaggcg cgcttatcca
     4261 ttgccaaaag catcaaggga gagcacgctc cgttcggtct tccaatcggt tgaaatcgcg
     4321 ctcttgaatc tccacgacct ggttacgcgg gccgctgacg acgtagccgg cgggagagcg
     4381 gcatcggcgt gcgtgaaatt gttctggatg cggggatttc atcgcttgtt gaaccggctc
     4441 agcatgattc cgcagcaact cgggatcggg caaatggagt ccgtgaacgg agggatctta
     4501 cgcattgccg actctcctgc cttcaacaat tactgtgaag cgcttcaccg cttcgatgcg
     4561 agcgtgatcg agtcgatcga cagcggcgct ctggacgcag agcacgccgt ggcggaccgt
     4621 tccctggacg actacgagtt caacctcctg cacttggcga gggtctgcaa tcacgagtca
     4681 accatttggg agcgaaacct cgctgaagtg cgggtgcctg tccccgtggc gtcgtacagc
     4741 gagtttgttg tagccgaagg catgcgaaac gcggtatttg atcgtgtcct ttcaggagat
     4801 acatacttca cgcagttccg tggacttcac cagattcccg aaacgctggg cgaagagatc
     4861 aacgaccgtt gtgaagaagc cattcgggat atccgaacaa cccgcttgcg tcacgcggtg
     4921 gagcatctcg attgcattca cgtgctgtcg gagggagtgc tggcggccgt acctccgatg
     4981 gcggaccaac tcgccactgc cgattatcat cagatccgag agaacctggg tctgaccagc
     5041 ggcagtcatt ccgtctgtct gcgataccac atgtttacgc atttgtatga gcagctgtgg
     5101 gacgaatatt gcacctgcgt gaccggcaag accgccaacg cccgcaccga cgtggaagtg
     5161 gaagaggcgc ttcgcgccct ggagggcaac tgccaggaag atacggtggc ctggaccatg
     5221 catctgttgg gcaattgctg cctgaaattg cgcgccttta ttagcgcctg gc
//
LOCUS       DASTMK010000474        28611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_248463, whole genome shotgun sequence.
ACCESSION   DASTMK010000474 DASTMK010000000
VERSION     DASTMK010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 28611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28611
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248463"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFQ79_19865"
     CDS             complement(<1..490)
                     /locus_tag="VFQ79_19865"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEU0141990.1"
                     /translation="MPLPSPSGVLCCGNIVFDILVKAVDAFAWGTTTWVDSIGQSIGG
                     NGANTTFTLARLGVPAGLIGPAGNDPAGERMIELLKDAGADISRIERLEEQTATTVAL
                     VRSDGARAFLHRPGVSSMAFENPVSLDGISEEYSHFHLANVFALPKLRRTAEETLRNA
                     LDQ"
     gene            complement(491..1414)
                     /locus_tag="VFQ79_19870"
     CDS             complement(491..1414)
                     /locus_tag="VFQ79_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141991.1"
                     /translation="MRYRFGPWQLMLGVVFLCAALIGVMFYHRSHRDLSDAELFAHLP
                     ENASLTIFIDLKSMRRAGILDMLAGSKLVEEADYQKFVERTGFDYRTDLDAVFAGFRD
                     GKNYYLLKGRFDWNSLKRYAIQEGGRCQNGLCYMDASSSGRYISFMPLRSRVLAMTVG
                     PDQHEARRLAENDSEQPYPDLPGAPVWAIASPQALGNSGSLPPGTRLFAKALENAEHI
                     TVSLNPQGNQFQARMRVVCRTPEDAVVLGAQLEKVTDVLRKLIQRSDQAPNPNDLSGV
                     LTAGVFRRDNRVVYGQWPIERSFLESVAGGS"
     gene            complement(1462..2499)
                     /gene="aroF"
                     /locus_tag="VFQ79_19875"
     CDS             complement(1462..2499)
                     /gene="aroF"
                     /locus_tag="VFQ79_19875"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU0141992.1"
                     /translation="MIISMKPDARQEEIEYVCERIREYGYKVHSIQGEQRVVIGAVGV
                     GDVTACLESLEALPYVEKAVRISAPYKFVSKEFNPGRTVISFDGLTIGGDEFVMMAGP
                     CSIENERQIMETAEAVAEAGARVLRGGAFKPRTSPYDFQGLEREGLKLLAKAREATGL
                     AIITEVMSERDVDLIAEYADVLQIGARNMQNFVLLKTLGKCGRPVLLKRGMSSTIKEL
                     LMSAEYVVAHGNPNVILCERGIRTFETATRNTCDIAAVSVLNELTHLPVVLDPSHATG
                     KRSLVPALARAGVAIGADGLLVECHPCPEKAVSDGAQSLDIEQFRQMMRDLQPYLELW
                     RANRVPEPAAV"
     gene            complement(3042..3995)
                     /locus_tag="VFQ79_19880"
     CDS             complement(3042..3995)
                     /locus_tag="VFQ79_19880"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU0141993.1"
                     /translation="MLVGQSTLILLLLIPVCAAAEDRRTPIILDTDIGTDIDDVFALA
                     LALASPEVDLRAVTTVSADAYGRALIACRFLESVGRADIPVAAGRPQRAKPEEKAQYQ
                     YGLEPSFRKRPVSELTPQFLYRQLKAQPGKLTLVTIGDLTNLGLLLTQHPEAKPWIKR
                     VVMMGGAVRVGYNAKPPVVWEWNIRSDIKAAQAVFSSGIPIVMAPLDATTMLKLEEPM
                     LNRIFGAGTGLSSQLHALYRLWGKTTPTLFDPVAVTLTFNESFCAMENLRIEVDDRGF
                     TREMPGEPNARVAIAIQKDRYLEWYCDRVTRGLPASGAKGR"
     gene            complement(4050..7754)
                     /locus_tag="VFQ79_19885"
     CDS             complement(4050..7754)
                     /locus_tag="VFQ79_19885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0141994.1"
                     /translation="MAIPHLIHTYFIVLYVVLIILIVSPAWPQASTSTVRGTVLDQAG
                     AAIANGSVFLTNTSTNITIKTSTNEVGFYMFPGVVPGPYELSIEAPGMQRFVVALTVQ
                     VQQAAVVDAVMQVGTTTTKVEVMDVTPMLVVDNPTLGHVLERTRIEQLPINSRNVFNL
                     LQTVPGLEQTWRAYGLRSGSQEIVLDGAYQSDRLWGGGQRRPPGLDTIQEFKVETNNS
                     SAKFTRPTTIVMLTRSGTNDIHGSLFETHRSNFKLGPFAGKARARTDYFDRAPKLVRN
                     EFGGTVGGPVVLPKLYNGRDRTFWFFSYEGQRMTNPATRSGSVPTEAMKNGDFSGLVD
                     SQGRQFKLYDPWTTDPATWQRQQFAYGGRPNVIDPARQSPLGKYLFSITPAPTIPNVN
                     PLVEPNWFGPVPNTQRQWTITGRFDHNFTENDRFYARWTQGDHDTLRYPFGTIPMIDW
                     VPGSVTNQSPNKSLALSWMRTFSPTLFNELLVSGSREHWTDSTGEAGVKYADRLGLPN
                     PFGVAGWPGIYDWSLRNYYFETGNPQATAFTYYVIGDNATKVVGKHELQFGFHFRYDQ
                     LNYLPDQQHPQGNHDTDTRGTALFDPASSPTNPLPAPFTGHTLGNLYLGVMNYSNQFV
                     RGYFYARGKEYSLYFQDNYKVTPRLTLNLGLRWEYWPAFTEKNNVITTFDPQRKAIVL
                     GQDLPGMYRIGATLPSIVDRLQFLGMNFTDYKEAGYPKNLRKNYWKDFGPRLGFAYRT
                     GDSQNAFVLRGGYRVSYFPTPMYTWGQRMRQNPPLTARFTNYLTDAAQAPDGIGNYGM
                     RSVPAVIAGQNSREAVKLSDPRGLTRGGALVSYFANEQPDSRVQDWNFTIEKEVMEDT
                     AAKVSYVGNHSDRLEQFYRYNESTPEYLWYVTTGERLPTGEYSGVLRRPFDKVVYGTI
                     EEFRKTGWSNFQGIQLELERRYSKGYGYQIFYVMSNALRAGGEGYGGTSYLPDTNMFL
                     PGRVPTDMDERNRFLNYSRDTGIPKHRVRWNWIVDLPFGKGKPLGGNAGPVLHRFIGN
                     WQVAGIGSLWSTYFSLPTGVYPNGNAIEIYGYKYPIQDCRSGVCYPGYLWWNGYIPAN
                     QINSVDPATGKPNGVMGVPSNYKPAGEPLLPWPASPNESDPLYNYYGTNTVWVPLNDG
                     SVQRVGFGDNLHPWRLQYFPSTRQWSLDASLFKTIPIRERFYLRFTADFFNVLNHPGN
                     PSSVESDGILSTRYSGISARELQFSLRLNW"
     gene            complement(7859..8359)
                     /locus_tag="VFQ79_19890"
     CDS             complement(7859..8359)
                     /locus_tag="VFQ79_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0141995.1"
                     /translation="MEVTAGLGRAGSATSKHTSSGGVNNPPLAVPAIDGKTTQVHKRR
                     GKVALVDFTTRPNCKQPSAGSQVLYQESGEKGFLPVAIVAGAQAWRALPIYRSLCRLT
                     FPVDIVEREEVLRYLNHPADKPMFVPILVLLAKQGRISMKEIGWIGEQKLRSANTGAG
                     YSTPLN"
     gene            complement(8577..8801)
                     /locus_tag="VFQ79_19895"
     CDS             complement(8577..8801)
                     /locus_tag="VFQ79_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEU0141996.1"
                     /translation="MDYNRIITIEPGKMGGKPCIRGMRITVYDVLDYLASGMTEEEIL
                     TDFPDLTREDLRACLAFAADRERRLVSIPA"
     gene            8918..9970
                     /gene="tdh"
                     /locus_tag="VFQ79_19900"
     CDS             8918..9970
                     /gene="tdh"
                     /locus_tag="VFQ79_19900"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU0141997.1"
                     /translation="MSELMPALVKPNGSRGLTLDSVPKPVPQRNEVLIRILKTGICGT
                     DLHIFQWDEWAQRRLHPPLIIGHEFMGVVEAAGDGCTQNFKPGDYVSGEGHLGCNQCY
                     FCRTGQGHICRTVQIIGVDCDGCFARYMVMPEGNLWRLKPEIPHEVAAIFDPYGNAMH
                     TVMAQPVAGRTLAIIGAGAIGLIACKIAEAAGALSVTVVEPQPRKRELALSYGAQFVF
                     DPREDGWKQRLLGSTPDYVGMDVVLEMSGSVAGIHDAFDLVRPGGDVALLGIPSREVQ
                     LDLSGAIIFKGVTVRGINGRLMFQTWYQCERFLVDHKLDLRPVITHRFPYRDYEQAFA
                     VLEQGEGVKVLLDWED"
     gene            9975..11177
                     /locus_tag="VFQ79_19905"
     CDS             9975..11177
                     /locus_tag="VFQ79_19905"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU0141998.1"
                     /translation="MTDPLSYLGDQLNAWRQAGTFQTLRQLQTACEPVSRFDGREVIN
                     LASNNYLGLANHPKLVEAALDATRRLGVGSGAVRTISGTMAIHMELEQKIARFKNVEA
                     CVVFQSGFAANAGTVSAILGPDDHIISDQLNHASIIDGARLSKAKIHVFPHKDVAAAG
                     RILSELDGAPGRKLLISDGVFSMDGDIAPLPELTAVAERHSAIMMVDDAHASGVLGRA
                     GRGTIDHFGLHGRVHVQVGTLSKAVGVLGGYVCGSRDLIDFLYHRARPFLFSTSHPPA
                     VAAACMAAFEIMENEPERIEALWDNTKYFQAALASAGFDTGASETPITPILVGEAKTA
                     HAFSRALFEEGLFATGIGYPTVPEGKARIRTIVTATHTREQLDRAVEILARVARRMNI
                     LRAPLPAA"
     gene            11289..12170
                     /locus_tag="VFQ79_19910"
     CDS             11289..12170
                     /locus_tag="VFQ79_19910"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0141999.1"
                     /translation="MKCDLHVHTVHSGMCTVPLARMFCRESYTQPNAAYGKLKRLGMD
                     LVTITDHNSIGACEVLRSHPDFFLSEEVTCQMPGGRELHIGVYDLNERQHIEIHRRAS
                     DIFSLVSYLREQNLFFSVNHVFSRLTGQRDTADFDLFDRFFPAVEILNGAILPVANRN
                     AELLARRMGKSGTGGSDAHTLAGVGSVYTEVPGARSKSEFFQGLRLGHSIVRGKHGGF
                     FKLTADVFQICCEMMAERPWTRLTAPVLAALPGVLLLNYLAETHFACDLQRRLERAWM
                     SPNSAVPVGAGTLPESV"
     gene            12167..12727
                     /locus_tag="VFQ79_19915"
     CDS             12167..12727
                     /locus_tag="VFQ79_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0142000.1"
                     /translation="MTTHQTILSFIVSKDHSLMRQVNHWSAPQWIRVWMICATRGGDG
                     WVWYALGLVILLFGGPERFLAVGAACLASATGIVLFLKLKKITGRKRPCMLEPHCWAK
                     LLPPDQFSFPSGHTITAFAISVPLSLFFPALLVGLLFCALSIAASRILLGMHFLSDVV
                     AGAVIGASLGYSSFLLFAAFQGGRPV"
     gene            complement(12683..13753)
                     /locus_tag="VFQ79_19920"
     CDS             complement(12683..13753)
                     /locus_tag="VFQ79_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142001.1"
                     /translation="MFCRILACGTLAGATPPPVGELDRQTLAWMQSVLDYWETTCSRH
                     LQVSAGPLPWIIFYDENRALHLNPEKRLLPSHRKVPSFLTFSGRTYPLVEVRHRGGLW
                     IPDGQALSVTSKAFTMLYAGDQKPFVTMPLPAFYRKHSGAGPAPVPDELFLGLSQHEL
                     AHTRQIVHAAKHIKRLRTRYQLPDDIDDDIIENTFGKNREYAKMYEEERRHLSRAVFA
                     HDLSLCREALAEALSAGQRRRERFFSGTRRFYADLEDIFLSMEGLGMWVHYQVARDRA
                     PQDEPWHQTLFQVLQTAGDSWSENHGLKLFVLMDRLVPGWQRRFLSREFPQPSEVLRE
                     ALKTDILSSHRTAALESGEKQE"
     gene            13831..15402
                     /locus_tag="VFQ79_19925"
     CDS             13831..15402
                     /locus_tag="VFQ79_19925"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU0142002.1"
                     /translation="MPSPQARKILLSRKWLIAFTVVALVLLTFLAALFHAASSLSRDY
                     VVEALSEKYQSDVELEELNLSLLPSIRATGHRLSFRHKGRTDVPPLIEIQEFRVQAGI
                     IGLLRSTKRVSKVELIGMKINVPPRREDKAESEEPKEKKSGGIKTRFVIEEIVADGTA
                     LHILPKDSWKEPLVFDIKRLTLTSVGIDRPMEFRAGLTNAKPPGDIESTGEFGPWVAG
                     DPGLTPVSGKYTFENANLGVFKGIGGTLSSVGKYKGALARIEVEGTTDTPDFVVDTAG
                     NPVHLKTSFHAIVDGTNGDTKLQPVNAEFLKTSVVARGGVEGKAGQKGKTVALDVQVS
                     EGRIEDLLELAVKSDEPVVTGAIRFTTAFELPPGDQAVMDKLRLKGRFGVEAAEFSSS
                     KVQEKIDELSNRARGEPERGSTGEVESDLKGNFTLANGMITLSGLSFSIPGATVHLNG
                     TYGLRGEEINFRGTVRMQAKVSETTTGVKSFFLKLADPFFKKKGAGAVIPIKIGGTRK
                     NPSFGLDAGRVIGGK"
     gene            complement(15405..16544)
                     /locus_tag="VFQ79_19930"
     CDS             complement(15405..16544)
                     /locus_tag="VFQ79_19930"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0142003.1"
                     /translation="MNTVMTRRELLAAGAAVALSKPAFSAPAAAVAIARCRTYSDGLE
                     SRMARMFDQIGGIGSLVKGKTVAVKLNLTGNPTRFPERADLPYRNNPDTVLALVHLLA
                     RAGARRVRLIESFFPARQDLDLWARYGLDIQAINNAGCKVEWENVQNKGQAANYVRIA
                     VPGGGYVFPAYHLNHSFVDCDVYASLSKLKNHWIAGVTMAMKNNFGNTPCSLYGGDAG
                     GEGNENPQQERGPVCHSGTKTPPKGVDQELHPDSPRDPGYRVPRVTADLVAVRPIDLS
                     IVDGVESIRGGEGAWNPGVQIIKPGLLLCGKNPVCVDAVSMAVMGYDPKAERGTKPFV
                     RGDNTLKLAEAKGIGTTDLARIEIAGLSIKEALHDYGPGPVGKPV"
     gene            complement(16566..17846)
                     /locus_tag="VFQ79_19935"
     CDS             complement(16566..17846)
                     /locus_tag="VFQ79_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0142004.1"
                     /translation="MSVRIAPAALFLFAWPAWAQRHDVNYEESKVPAYILPDPLLTLD
                     GEVVQDAETWNNVRRPEILRLFESEMFGYSPGKPRDMYFEPQSIDRNALGGSAIRKQV
                     RVYFSRQKNGPKMDMLLYLPKNAAKPVPLFIGLNFQGNHAIHPDAGITLSTAWMRPNP
                     KTGVVNHRATEQSRGAAQSRWPVDKILARGYGLATIYYGDLDPDFDDGFQNGVQPMFY
                     RPGQTKPGPDEWGSIGAWAWGLSRALDYIETDKDIDARRVAVLGHSRLGKTALWAGAL
                     DTRFALVISNDSGCGGAALSRRQFGETVHIINTAFPHWFADNFNRYNGNENALPFDQH
                     MLIALIAPRPVYVASAEEDRWADPKGEFLSARYAEPVYKLFGASGLGVDEMPPVNHPV
                     MDTVGYHVRSGKHDVTDYDWEQYLNFADKHLINK"
     gene            17937..20435
                     /locus_tag="VFQ79_19940"
     CDS             17937..20435
                     /locus_tag="VFQ79_19940"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEU0142005.1"
                     /translation="MILTMMMRCFLFLVPAFAFILCPSAQPKDVERHHVANLDSSPQQ
                     YNLRLEGTVDAANTRDPIVYAAWNQVFDPMRSVKVENTGDTDVINPWITVNGKRDWRT
                     TKAIAEEALRTYGDPATMSDGEKARAIWEFLRRNRFHATTGDFEVRDPVKMLNVYGFS
                     LCGDNAPALMDLWRSAGLKTRRGFPIGHCVAEAWYDGAWHMLDADESIIFLDRDNRTI
                     ASERAVARDHDLSKRAYEDEYLPTLYNYDGTRSGDYPAHTAHSMNFTLRPGESLEWRW
                     GQGSKHHYAPNPVLFLLKSSNLNEWGPKAWATLRNGKWAYTPPLTKPAGRKGAEARNI
                     RWLEGAGGLAAIPQAAGELAALTWEMQTPYVMVGGRLRARTRLQADDLVVFSFSQDGS
                     TWREIVRNQSQVDASLDAFFPSPGVAHYRYFIRAEFRAASDPEAVGLESITIENDLQM
                     APLAMPSLELGDNRILYRDETGGPRAVRATFEWVERSSLKPPDAPATPVFPKDGAEVE
                     GTAVVFEWTKPPSSEERPVADYHFELSDEPEMRWALSPAFDVLISKTKGKGGTRFALP
                     TAGLLNPGQTYYWRVRAKSEEGVWGAWSKTWSFVPQGPGIPLLPRLEERDPDAWTLAW
                     DASPHGRKPARFRVYASDEKGFTVSDRPYEVAVGNQKERGLYPGKKQVTFPANFVAET
                     GEPFLRIDPARAYYRVVAVDERGNVSGSSDFVEAPRPWIFSQPRMEATIGEPYRYEVK
                     TIRSIGDLSYRDFGPGQSYQAAYWDAEKPEFSIETEMPRCGNFDAKWLKIDPDTGVLS
                     GTPAAADTGEYQINVRVRVNGKEHVQSFPLKVNR"
     gene            complement(20437..21318)
                     /locus_tag="VFQ79_19945"
     CDS             complement(20437..21318)
                     /locus_tag="VFQ79_19945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0142006.1"
                     /translation="MNRRDLLCSLPALAAAPLLRAADEQVPGKARLRNAICAYSYREE
                     LKSQSMSYSDLVRVAVETGAEGLDLTVYWFPPDAPDSFLFPLKRLAFKNAVEIYSISI
                     RTDMCRPSGAVRDKEITEVRKWVDVAAKLGAGHIRVFGGTVPKGSMEDQAAQWAVETL
                     KPCVEYAGSRGVILGLENHGGITERAERIIQIVKQVDSPWLGINLDTGNFNRNVLPQI
                     EMSIPYAVNVQVKVEQRLEDGTRGPQDWDRIVQMLANANYRGYLALEYEARESAVSAV
                     PRLTRRLGEIIRKYSQA"
     gene            complement(21347..22237)
                     /locus_tag="VFQ79_19950"
     CDS             complement(21347..22237)
                     /locus_tag="VFQ79_19950"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0142007.1"
                     /translation="MALSRREFLAGLPAAALAQETGYAPMLASQVYVWMQQLSAEKKP
                     VSDNLERIFSGTARAGFQRLELDASFFKPELADETARRLRRHKLEVPIVYNGGPMHMR
                     ELAEATWAETLKVAEAAKIAGATAVNFNASPKPGGQRKSDAELKTQAYYIDQLAEELA
                     LRHMHLFLHQHAPEMAENAREWRHLLANTNSATVSFCVDVDWVLRGKQDPMTILEEGR
                     RRLASLHLRNSVNGVWTEAFGPGDIDYQNVADYLKATAYRGYLVVELAYVAETRKTRS
                     LEDNLKESRQYAEKIFEPGA"
     gene            22299..23495
                     /locus_tag="VFQ79_19955"
     CDS             22299..23495
                     /locus_tag="VFQ79_19955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0142008.1"
                     /translation="MILFWALVIPALLLSVLAYRGEAARIRYIKRILSEPDTGEFPPV
                     TVIVPVKGEDEGLRENLASLAALDYPDYELIIAARTPGDVPGGVLPPKAKVVYARAAP
                     FRAATVRERGLPGEDSSEKIGNLLAAVAASRAESGIYAFADSDGRVRPGWLRALAAPL
                     RRKEVGASTGYRWYIPRHPDFWSLLRGAWNAVIAGSFGPGPNGFLWGGAMAIRKQVFE
                     EARVAEYWAGALSDDYQLARAVLDAGLEIAYAPGAMVASVDRIGPRELFSWVRRQMVL
                     TRVHAPRLWRTALLAHVIYCGAMLACAAAALAGWMPAILLLGAQIGLGMGKALVRSRT
                     VRLCFPEHRDWFRRYAWMEVMLAPAATWLWLCSLLASAAGNTIEWRGRTYRLERPPRR
                     ARTTEM"
     gene            23580..24140
                     /locus_tag="VFQ79_19960"
     CDS             23580..24140
                     /locus_tag="VFQ79_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142009.1"
                     /translation="MITLAHRHLLRALIPSLIVAAAFSQTTPLPFKPVAFEYSKTLDR
                     LILISSNPNRLHIYDPSTSQTVSVTLAQPPLSLSVSPDGLRAAVAHDGLISYADLEKG
                     TLLKTFNVPAVITGIVVTSSWIYTVGNYYASLSSVNIASGEVIPNSAVFSMTAGKFNP
                     TVNAIYGTRDGTSPNDVESGIFRQAL"
     gene            complement(24260..24739)
                     /locus_tag="VFQ79_19965"
     CDS             complement(24260..24739)
                     /locus_tag="VFQ79_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142010.1"
                     /translation="MVTHGTRLIAWYTQLAGALIFTEPEVPSAGTSIELVANAAPHSP
                     PGAQRNALHGVVVQNSGRGVGLLDHDKFGRIGSEENCSSVRGIRFTASVKPGKRELAH
                     RQQIGTLEDLDYAFIYPIRIARPAAEEQRNLLKRGGLVQRPDLADAGYGGHVIHVKL"
     gene            24714..26552
                     /locus_tag="VFQ79_19970"
     CDS             24714..26552
                     /locus_tag="VFQ79_19970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU0142011.1"
                     /translation="MSLVPWVTIVSGGLGSGNGTLQWAVAANADSISRTGTIIVPGRI
                     FTITQAAVSSSNPSSLYIPGYYAVDAAYAKPIDKLVLVSANPNQLHIFDPAARRDTVV
                     QLNAPPLSLSVRPDGVQAAVGHDGWVTLVDLNTAAILNVISVPTDVHSLILAGNGYAY
                     LFPQQEWGDLFSLELSSGQIARTSAIYNGREPRLHVNGKNIYVGGQWQSKWDISGGVA
                     KLVNGWPSSGCGKLWLTEDGRRFFGDCGKAYTTSEVPAQDYQYNGSLSNATRIVWLDE
                     SAKAATIAAIPSAGPAYSSSAKSIDDTQVQLYGDAYLGYLGAIPLPKFIVGGTAFPAH
                     GRYVFWNQSGTGLYAVVQADSKALLTGGTAITELSVSDARQVAVKSVVNSASQSEGRI
                     APGELISIYGTGMGPQTGASFALDPVSGKANTSLAGTEVYINGLAAPILYASDTQVNA
                     IVPFAAPVSGQVILQVGTQGILSEGVTVTMTAAAPGVFTMNGSGSGQAVAVNLDGTLC
                     DSAHPAAAGSYITVYFTGGGSTTPVGSTGSVAGLALKRLSQTALATVADIPATVSFAG
                     AAPTLLEGVNQLNIKLSEETPPGLAQPLIVTVGNNSSPASATIAVK"
     gene            26646..28037
                     /gene="thiC"
                     /locus_tag="VFQ79_19975"
     CDS             26646..28037
                     /gene="thiC"
                     /locus_tag="VFQ79_19975"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEU0142012.1"
                     /translation="MRTQWIEKRKNDAVRTQMHYARRGILTEEMEYVARKERLSPEVV
                     REEVAKGRMIIPANINHRNLEPMCIGIASKCKINANIGNSATTSNIDEELEKLRYAVK
                     YGSDTVMDLSTGGDIPAIRRAIIDNSPVPIGTVPIYEALTRVRRIEDLTPQVMLEVIE
                     EQAGQGVDYMTIHAGVLVQHIPLTTRRVTGIVSRGGSILAEWMVKNHKQNFLYEHFDE
                     ICKIFQKHDVSFSLGDGLRPGSLADASDEAQFAELKTLGELTRRAWEYDIQVMIEGPG
                     HIPMDQIELQVDKERELCYDAPFYTLGPLVTDFAPGYDHITSAIGAAMIGWYGASMLC
                     YVTPKEHLGLPNREDVKAGIIAYKIAAHAADVARHRPGARDRDDELSRARYAFDWNRQ
                     FELALDPETARAMHDETLPEEGFKEAHFCSMCGPKFCSMNISSKVEDFTAADAEAVLS
                     GQPNRELVNLSGD"
     gene            28044..28466
                     /locus_tag="VFQ79_19980"
     CDS             28044..28466
                     /locus_tag="VFQ79_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142013.1"
                     /translation="MYELIRGAAGDRVLFLSLPEDTGLIARLAGEAVRGLLFALGADE
                     AVYAARIDFRDFQNVMFAPGEPDVIPWQDAFFTIAIDSSCEWRDAAATVRELARVLAG
                     DGVAYLKADERLKTELEAGGFLEVDSGGELRKFRKLTS"
     gene            complement(28456..28605)
                     /locus_tag="VFQ79_19985"
     CDS             complement(28456..28605)
                     /locus_tag="VFQ79_19985"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEU0142014.1"
                     /translation="MHYLTVHDFEELARQEKWNVERRIFLAGNRTGTLWPNLMAETAV
                     FLIRK"
BASE COUNT         5684 a         8305 c         8739 g         5883 t
ORIGIN      
        1 cctggtccaa tgcgttgcga agcgtctcct ccgctgtgcg gcggagcttg ggaagcgcaa
       61 aaacgtttgc caggtggaag tgcgagtact cttcagaaat cccgtcaagc gaaacaggat
      121 tttcgaaagc catggagctg acaccaggcc ggtgcaggaa ggcgcgcgct ccatcggaac
      181 gcaccagggc gactgtggta gctgtctgtt cttccaggcg ctcgatacgg gagatatcgg
      241 ctccggcgtc tttaagcaac tcgatcatcc gctctccggc cgggtcgttt ccggcaggtc
      301 cgatgagacc ggcgggtacc ccgagccgtg cgagagtgaa ggtggtattg gccccgtttc
      361 cgccgatgct ctgtccgatg gagtccaccc aggttgtggt gccccaggcg aacgcgtcca
      421 ccgctttgac gaggatgtca aaaacaatgt tgccgcagca gagaacaccg ctcggcgagg
      481 ggagaggcaa tcaggaacct ccggcgacgc tttcaaggaa agaacgctca atgggccact
      541 gcccatacac gacgcggttg tcccggcgga atactccggc cgtcagaacg ccgctcaggt
      601 cgttcgggtt tggggcctga tcgcttcttt gaatcaactt ccggagtacg tccgtgactt
      661 tctcgagttg agcgcccaac accaccgcgt cttccggcgt ccggcacact acacgcatac
      721 gcgcctgaaa ctgattgccc tgcggattca gagatacagt gatgtgttcg gcgttctcga
      781 gcgccttcgc gaataatcgc gttccgggcg gaagcgaccc tgaattgcca agggcctggg
      841 gagaagcgat ggcccagacc ggagcgccag gcaggtcggg gtaaggttgt tcgctgtcgt
      901 tttcggccaa acggcgggct tcatgctgat cgggtccgac cgtcatggcc agcactcgcg
      961 agcgcagtgg catgaaggaa atgtagcgtc cggaggaact ggcatccata tagcagagac
     1021 cgttctggca tctgccgcct tcctgtatgg cgtaccgctt cagtgaattc cagtcgaaac
     1081 ggcccttgag gaggtaatag ttttttccgt cccgaaaacc ggcgaatacg gcgtcgaggt
     1141 cggtacggta gtcgaagccg gtgcgttcaa caaacttctg gtaatcggcc tcttcgacca
     1201 gtttggagcc ggccagcata tccagaattc ccgcacggcg catgctcttg aggtcgataa
     1261 atatcgtgag agacgcattt tccggcagat gtgcaaagag ctccgcatcg ctcaaatccc
     1321 tatgggaccg gtgataaaac atcactccga taagggcggc gcacaaaaac acgacgccca
     1381 gcatcagttg ccagggaccg aaacggtagc gcattacacc atcaggttag cgcagcggcg
     1441 cgttcaagcg gtttcaatcg cctataccgc cgccggttcc gggacgcggt ttgcgcgcca
     1501 cagttcgaga tagggttgca ggtcgcgcat catctgccga aactgttcga tgtcgagaga
     1561 ttgggcgcca tcgctgaccg ctttttcggg gcaggggtgg cactccacca acaggccgtc
     1621 cgctccgata gcgacgccgg cccgcgcaag agcaggcacc aggctgcgtt ttcctgtggc
     1681 atggctcggg tccagcacca cgggcaagtg agtcagttcg ttcaaaacac ttacggccgc
     1741 aatgtcgcac gtattgcgtg tggcggtttc aaaggtgcgt atgccgcgtt cgcagaggat
     1801 gacattggga ttgccatgcg ccactacgta ttccgctgac atcaggagtt ctttgatggt
     1861 ggaactcatg ccgcgcttca gcaataccgg ccggccgcat ttacccagcg tcttgagcag
     1921 aacgaagttc tgcatgttgc gtgcgccaat ctgaaggacg tcggcgtatt cggcaatcag
     1981 gtccacatcg cgttcgctca tcacctcggt gataatggcc aggcccgtag cctcgcgagc
     2041 tttagccaac agcttcagcc cttcgcgttc gagcccttga aaatcgtaag gcgaagtacg
     2101 cggcttgaag gctccacctc gcagcactcg cgcgccggcc tcggcgaccg cttcagccgt
     2161 ctccataatt tgccgttcgt tttcaatgga gcatgggccg gccatcatga caaactcgtc
     2221 gccgccgatg gtcaacccgt cgaaagagat gaccgtccgg ccggggttaa attccttgct
     2281 gacaaacttg tagggggccg aaatccggac cgccttttca acgtacggta aggcttcgag
     2341 agactcgagg caggcggtta cgtcgccgac tccgacagcc ccgatcacga ctcgttgctc
     2401 cccttggatg gaatgcactt tgtatccgta ttcccgaata cgttcacaga catactcgat
     2461 ttcctcctgc cgcgcgtccg gcttcatgga gatgatcata tttgccccct aaaaagcgaa
     2521 aacccactca ttgctgagtg ggttggttca ctacgtgttc cgaaaattcg tcttccaaca
     2581 ccacccggcc ggctcaggcg aaacgccaga accggaacgt aaacgagtag gtgaaggaaa
     2641 ctaccataga aacttgaatt ttagctctgg acgaatcccg aatgcaagag aagaaatgtg
     2701 aagggcatct aatctcatac aagatgagcc tgaaggggga ccgatgatgc tggggattga
     2761 ccatcacggt ggaagccaga tgttgcgggg tcgcgcagtt tctaccggct gcgcaagaca
     2821 cgggcctatt gccaacggca cttggtggat acggtccacc cggacgatcg ggatgggcgg
     2881 gatctgacca ccacctatgt ggagggagaa gcggaacaga acccgaaggc caaacggggt
     2941 tacagccggg atggatggcc agacatcatg ggaagacccc agtcaatgta gccttcgacg
     3001 ttgaatggat gatccgccga ggcggcgagc ggcttccgtg ttcatcgccc tttggcgccc
     3061 gaggcgggca ggccccgcgt gacccggtcg caataccatt cgaggtacct gtctttttga
     3121 atggcaatgg ctacacgggc gttgggctcg cctggcattt cgcgggtgaa gcccctatcg
     3181 tctacctcga tccgcagatt ttccatcgca caaaagcttt cattgaaggt gagcgtcact
     3241 gccaccgggt cgaacagcgt gggtgtcgtt ttcccccaga gccgataaag cgcgtgcaac
     3301 tggctactca agccggttcc cgccccaaag atgcggttta acatcggttc ttccagcttg
     3361 agcatggtgg tggcatccag gggagccatt acgatgggga tgccggagga aaaaactgcc
     3421 tgtgccgcct taatgtcgga acgaatgttc cattcccaca ccacgggtgg tttcgcattg
     3481 tagcccacgc ggactgcacc tcccatcatc acgacccgct tgatccacgg cttcgcttcc
     3541 ggatgctgcg tgagcaggag gcccaggtta gtcagatcgc cgatggtcac gagcgtcaac
     3601 ttgcctggct gcgccttgag ctgccggtaa agaaactggg gagtgagttc ggaaacgggt
     3661 ctcttccgga atgacggctc caggccgtac tgatattgtg ccttctcctc gggctttgcc
     3721 cgctgaggac gccccgcagc tacgggtata tcggcccgcc ccactgactc gaggaaccgg
     3781 caggcaatca acgcccggcc gtaggcgtcg gcgctgaccg tcgtgaccgc gcgcaaatcg
     3841 acttcaggac ttgccaaagc aagggccagt gcaaagacat cgtcaatgtc cgtgccgatg
     3901 tcagtatcga ggatgatcgg cgtccgccgg tcctcggcgg cggcacagac aggaatcaac
     3961 agcaaaagaa taagcgtact ttgacccaca agcatttctg aaacgtgacc tcgcggggcc
     4021 agtcccggaa gactggccca ttttgcagcc taccagttga gccgcaagct gaactgaagc
     4081 tcgcgcgctg agatgccgga gtaacgcgtg ctcaagatgc cgtcactttc gacgctggac
     4141 ggattgcccg gatggtttag cacgttgaag aagtcggcgg tgaagcgcag gtaaaaacgc
     4201 tccctgattg gaatggtctt gaacagcgat gcatccaggc tccattgccg ggtgctcggg
     4261 aaatactgga ggcgccacgg atggaggttg tcgccgaacc ccacccgctg caccgaacca
     4321 tcgttgagcg ggacccacac ggtgttggtt ccgtagtaat tgtacaaggg gtcgctttcg
     4381 ttcgggctgg ccggccacgg caaaaggggc tcgcccgccg gtttgtagtt cgacggcacg
     4441 cccatcaccc cgttcggttt tcccgtcgcc ggatcgacgc tgttgatctg gttggccgga
     4501 atgtagccgt tccaccagag gtagcccgga tagcagaccc cgctgcggca gtcttggatc
     4561 gggtacttgt agccgtatat ctcgatcgcg ttgccgttcg gatagacccc cgtgggcaga
     4621 gagaagtacg tgctccacaa ggaacctatg cccgccacct gccagttgcc aatgaaccgg
     4681 tggagaacgg gaccggcgtt gccacccagc ggcttgccct tgccgaaagg cagatcgacg
     4741 atccagttcc aacgcacccg gtgcttcggg atgccggtgt cgcgcgagta gttcaggaag
     4801 cggtttcgct cgtccatgtc cgtgggaacc ctgcccggca ggaacatgtt ggtgtcggga
     4861 aggtaagatg ttccgccgta gccctcgccg ccggctctca gggcgttgct catgacgtaa
     4921 aagatctgat agccgtagcc cttgctatac cggcgctcca gctccaactg gatgccctgg
     4981 aagttagacc agccggtctt acggaactct tcgattgttc catagacgac tttgtcaaag
     5041 gggcgccgca ggacgcccga atactcgccc gtcggaagcc tctctccggt cgtgacgtac
     5101 cagaggtatt caggagtgct ttcgttgtac cggtagaact gctcgaggcg gtccgagtgg
     5161 ttgccaacat atgagacttt tgcggccgtg tcctccatga cctccttttc gatggtgaag
     5221 ttccaatcct gcactcgcga gtcgggctgt tcgtttgcga agtagctcac aagagcgcct
     5281 cctcgcgtca aaccccgcgg gtcagaaagc ttcacggcct cgcggctgtt ctggcctgcg
     5341 atcacggccg gcaccgagcg catgccgtaa ttgccaatac cgtcaggcgc ttgggcggcg
     5401 tcagtcagat aattggtgaa ccgcgccgtg agcggggggt tctggcgcat cctctggccc
     5461 cacgtgtaca tcggagtcgg gaaataggag acgcggtagc cgccccggag gacaaaggcg
     5521 ttctggctat cgccggtgcg gtaggcaaag ccaagccgtg ggccgaaatc cttccaataa
     5581 ttttttcgca ggttttttgg gtagccggct tccttgtaat ccgtgaagtt cattcccagg
     5641 aattgaagcc ggtcgacgat cgaaggcagt gtggctccaa tccggtacat gcctgggaga
     5701 tcctggccga gaacgatcgc cttgcgctgc gggtcgaagg tggtgatgac gttgttcttt
     5761 tcggtaaacg ccggccagta ttcccagcgc agtccgaggt tcagcgtcag ccgcggagtg
     5821 accttgtaat tgtcctggaa gtagagcgaa tactccttgc cgcgcgcgta gaaataaccg
     5881 cgcacgaact ggttggaata attcatcacg ccgaggtaca ggttgccgag cgtatgcccg
     5941 gtgaagggcg ccggcaaggg gttcgtaggc gaagatgccg gatcgaagag cgcagtgcct
     6001 cgggtgtccg tatcgtgatt accttgggga tgctgctgat caggaaggta gttcaactgg
     6061 tcgtagcgga agtggaagcc gaactgtaat tcgtgcttgc ctacgacttt ggtcgcattg
     6121 tcgccgatca cgtaataggt gaacgccgta gcctgcgggt tgcctgtctc gaaatagtag
     6181 ttccggagac tccaatcgta gatccccggc cagccggcaa ccccgaaagg attgggcagc
     6241 cccagcctat cggcgtactt gacacctgcc tcgccggtcg agtcggtcca gtgctcccgg
     6301 gatccgctca ccagcagttc gttgaacagc gtcggagaga acgtacgcat ccaggacagg
     6361 gccaggctct tgttcggcga ctggttggtc accgaccccg gtacccaatc tatcatggga
     6421 atcgtgccga acgggtaccg cagcgtgtca tggtctccct gcgtccagcg cgcataaaag
     6481 cggtcattct cggtgaagtt gtggtcgaaa cggcccgtta tggtccattg gcgctgggta
     6541 ttgggcaccg gaccgaacca gttcggctcc actaggggat tgacgttcgg gatagtcggc
     6601 gctggggtga tgctgaacag atacttgcca agcgggctct ggcgggccgg gtcgatgacg
     6661 ttcggccggc cgccataggc gaactgttgc cgctgccagg ttgcggggtc tgtggtccat
     6721 ggatcataca gcttgaactg cctgccttgg ctgtccacca gcccgctgaa gtcgccattc
     6781 ttcatcgcct cggtcgggac cgatcccgat cgtgttgccg gattcgtcat ccgctgtccc
     6841 tcgtaggaga aaaaccagaa cgtgcggtcc ctgccgttgt aaagcttcgg gagaaccacg
     6901 ggaccgccca cggtcccgcc gaactcgttg cgaacgagct tcggggccct gtcaaagtag
     6961 tctgtgcggg cgcgcgcctt tccggcaaag ggcccgagtt tgaagttgct gcggtgcgtc
     7021 tcgaacagtg atccgtggat gtcgttggtg ccgctgcggg tcagcatgac gatggtggtc
     7081 ggacgagtga acttcgccga agagttgttg gtttccacct tgaactcctg gatggtgtcc
     7141 agccctggcg gccgcctctg gccgccgccc cataggcggt cgctctggta tgccccgtcc
     7201 agtacgattt cttgggaacc cgagcgcaga ccgtaggcac gccaggtctg ctccaatccg
     7261 ggaaccgtct ggagcaggtt gaaaacgttc cggctgttga tgggcaactg ctcgatacgg
     7321 gtgcgctcca ggacgtgacc gagtgtcgga ttgtccacga caagcatcgg cgtcacgtcc
     7381 atgacctcta ccttggtggt cgtcgtgccg acctgcatga ctgcgtcaac aaccgctgct
     7441 tgctgcactt ggacagtcag ggccaccaca aacctctgca tgccgggcgc ctcgatcgac
     7501 aattcatatg gcccgggaac cacgcccggg aacatataga agcccacctc gttggtggac
     7561 gtcttgatag tgatgttggt agacgtattg gtgagaaaga ccgagccgtt cgcgatagct
     7621 gccccggcct ggtcaagcac ggttccgcga accgtgctgg tgctcgcctg cggccaagcg
     7681 ggcgaaacga tcaatataat aagcacaaca tacagaacta taaagtatgt gtgaatcagg
     7741 tgtggaatcg ccattatgga cctccctccg ggcggccata gcgctcacct aaattcaccc
     7801 gcctcagctc ccagatccgg cggaccaccg aggcaatttc ttacttgaat agaatcgatc
     7861 aatttagcgg agtgctatag ccggcacctg tattggcgga acggagtttt tgctctccaa
     7921 tccagccgat ctccttcatc gaaatgcggc cctgttttgc cagcaggacc aggatgggta
     7981 cgaacatggg tttgtcagcc gggtgattaa gataccgaag gacctcttct cgctcgacaa
     8041 tgtcaaccgg aaacgtgagg cggcacaggc tcctgtaaat cggcagtgcc ctccacgctt
     8101 gcgcgccggc cacaatcgcc accggcagga agcccttctc gccagattcc tggtagagca
     8161 cctggcttcc cgcggatggc tgtttgcagt tcggacgcgt cgtgaagtcc accagcgcaa
     8221 ccttgccgcg ccgcttgtga acctgagttg tctttccgtc aatggccgga actgccagcg
     8281 ggggattgtt gactccccca gaggaagtgt gtttggatgt agcggaacca gcccgtccca
     8341 agccggccgt gacttccact cctgccgcat tccgcagcca attccgccgg ctcagattaa
     8401 gctcacctgc cggcgatcga acgcattgtg caatcgcagg caaaaattca aatccaattt
     8461 aagttaccag agactgccaa ctagatcaag ctgagaaatg tgagaaatgt gcgttacgag
     8521 gccagactac gtctccactg ccgggttttg taggatagat tcagcgaaga ggagcctcaa
     8581 gcggggatgc tcaccaggcg gcgctcacgg tccgcggcaa aggcaaggca agcacggagg
     8641 tcctcgcggg tgaggtcggg aaagtcagtg aggatctctt cttcagtcat gcccgaggcg
     8701 agatagtcga ggacgtcgta gaccgtgata cgcatgcctc ggatgcacgg cttgccgccc
     8761 atcttacccg gctcgatcgt gatgatcctg ttataatcca tcggtccttt tcacaacgcc
     8821 ctctattagc atagctgggt ttgccaggtt tgaagggcat gtaaagataa tattattgga
     8881 gagacaagct cccctctaat tcgcccgcac aacgactatg tctgaactca tgcccgccct
     8941 ggtgaagccg aatggctccc gcggacttac actcgatagc gttcccaagc cggttccaca
     9001 acgaaacgaa gttttgatcc gcatcctcaa gactggtatc tgcggaaccg acctgcacat
     9061 ctttcaatgg gatgaatggg cgcagcgccg cctgcacccg cctctgatca tcggacacga
     9121 gttcatgggt gttgtggaag ccgccggtga cggatgtact cagaatttca aacccggaga
     9181 ttatgtctcg ggtgaaggac acctcggctg caaccaatgc tatttttgcc ggaccggaca
     9241 gggtcatatt tgccgcacgg tccagatcat cggcgtggac tgcgacggct gctttgcgcg
     9301 ctacatggta atgccggagg gtaatctctg gaggctcaag ccagagatcc cacacgaagt
     9361 cgccgccatc ttcgacccgt acggcaatgc gatgcacacg gtgatggcac aacccgttgc
     9421 ggggcgaact ctcgctatca tcggcgccgg agccattggt ttgattgcct gcaagatcgc
     9481 tgaagccgcc ggagcgctct ccgttacggt tgtggagccg cagcctcgca aacgggaact
     9541 ggccctctcc tacggcgcgc agtttgtttt cgatcccagg gaagatggat ggaaacagag
     9601 gctgctggga agcacgcccg attatgtcgg catggacgtt gttctggaaa tgtccggcag
     9661 cgtcgcggga attcacgatg cctttgatct tgtgcgcccg ggcggtgatg tggcgctgtt
     9721 gggtataccg tcgcgcgagg tgcagttgga cctttccggc gccatcatct tcaaaggcgt
     9781 cactgttcgc ggcattaatg ggcgcttgat gttccagacc tggtatcagt gcgaaaggtt
     9841 cctggtggat cacaaactcg acttacgccc tgttattaca caccgcttcc cgtatcggga
     9901 ctacgagcag gcgttcgcgg ttttggaaca gggtgaaggc gtcaaagtgt tgcttgattg
     9961 ggaagactga agcgatgacc gatccattat cttatctcgg agatcaattg aacgcctggc
    10021 ggcaggcggg gaccttccag acactacgcc agctccagac cgcctgcgag ccggtgtcgc
    10081 gttttgacgg acgcgaggtc atcaacctcg catcgaacaa ctatctcggc ctggccaatc
    10141 atcccaagct ggtggaagcc gcgctcgatg ccacgcggcg cctgggtgtg ggctccgggg
    10201 cggtgcgcac catctcgggc accatggcaa tccacatgga actcgaacag aagatcgccc
    10261 ggttcaagaa cgtcgaggct tgcgtcgtat ttcagtcggg gttcgcggcg aatgccggga
    10321 ctgtatcggc gattctcggt ccggacgatc acatcatctc ggaccaattg aatcacgcga
    10381 gcattatcga cggtgcgcgt ctgtcgaaag cgaagatcca cgtgtttccg cacaaagatg
    10441 tcgcggcggc cggccgtatc ctgtcggaat tggacggagc acccgggcgc aagctgctga
    10501 tcagcgacgg cgtattctcc atggatggcg acatcgcgcc actgccggag ctgacagccg
    10561 tcgcggaacg ccacagcgcc atcatgatgg tggacgacgc gcatgcctcc ggcgttctcg
    10621 ggcgtgcagg gcgcggcacc atcgatcatt tcggattaca cggcagggtc cacgtgcagg
    10681 tcggtacgct ttcgaaagcg gtcggcgtgc tgggaggata cgtgtgcggc agccgcgatc
    10741 tcatcgactt cctttatcac cgcgcgcgtc cgtttttatt ctcgacctcg catcctccgg
    10801 ccgtagcggc ggcgtgcatg gcggccttcg agatcatgga gaacgagccg gaacggatcg
    10861 aggcgctctg ggataacaca aagtacttcc aggcagcgct cgcttccgcc gggtttgaca
    10921 cgggcgcgag cgaaactccc atcactccca tcctggtggg cgaagcgaaa accgcgcacg
    10981 cattctcgcg agcgctgttt gaggaggggc tgtttgcgac aggtatcggc tatcccaccg
    11041 tgcccgaagg gaaggcccgc atacgcacca tcgtgacggc tactcacacg cgggaacagt
    11101 tggaccgggc ggtggagata ttggctcgcg tcgcacgccg tatgaacata ctgcgcgcgc
    11161 cgcttccggc agcctagcac cgtacagttc ggcgcttcag gctgatccaa tataaacatt
    11221 ctctttaata gatgtttttt atggatgtca tggtactgta gcaagccgcg gctacgctgt
    11281 aggcaggtat gaagtgcgac ctgcatgtcc acacggtgca ctcgggaatg tgcacggtgc
    11341 ccctggcgag gatgttctgc cgggaaagct acacgcagcc caacgcggct tacgggaaat
    11401 tgaagcgttt gggcatggat ctggttacga tcacggatca taattcgatc ggtgcctgcg
    11461 aagttcttcg ttcccatccc gatttcttcc tcagcgagga agtaacctgc cagatgcctg
    11521 gcggccgcga attgcatatc ggcgtctacg atttgaacga gcggcagcac atcgagatcc
    11581 acaggcgggc ctccgatatc ttttcgctgg tctcgtatct gcgtgagcag aacctgtttt
    11641 tcagcgtaaa tcacgtattt tcgcgcttga cagggcagcg cgacaccgcc gatttcgacc
    11701 tgttcgaccg ctttttcccg gccgttgaga tcctcaacgg agcgatcctg ccggtggcaa
    11761 accggaacgc ggagttgctg gcacgccgga tgggcaagtc cggcacgggc ggcagcgacg
    11821 ctcacacgct ggccggagta ggttcggttt atacggaggt tcccggcgca cggtcgaaga
    11881 gcgagttctt ccaggggctg cggctgggac acagcatcgt acggggtaag cacggcggtt
    11941 ttttcaagtt gactgcggac gtgtttcaga tctgttgcga aatgatggcc gagcggccgt
    12001 ggacaaggct gacagcgccg gtgctggcgg cgcttccggg agtcctgttg ctgaactatc
    12061 tggcggaaac gcatttcgcc tgtgatctgc agcgccgttt agagcgcgca tggatgtccc
    12121 cgaacagcgc ggtgccggtg ggcgccggaa ctctgccgga gtctgtatga ccacgcatca
    12181 gaccatcctg tccttcatcg tttccaagga tcacagtttg atgcgccagg tgaaccactg
    12241 gagcgcacct cagtggattc gtgtgtggat gatctgcgcc acgcgtggag gagacggttg
    12301 ggtctggtac gcgctcgggc tcgtgatcct gcttttcgga gggccggaac gattcctggc
    12361 agtgggcgcc gcctgcttgg cgtccgctac aggaatcgtt ctgtttttga agctgaagaa
    12421 gatcaccgga cgaaaacggc catgcatgct ggagccgcat tgctgggcga aactgctccc
    12481 gccggaccag ttctcgtttc cctccggcca taccattact gccttcgcga tatcggtgcc
    12541 gttgtcgtta ttctttcccg cgttgctcgt aggcttgctc ttctgcgcgc tgagcatcgc
    12601 cgcatcccgc attctgctgg gcatgcactt tctgagcgac gtagtcgccg gggccgtcat
    12661 cggcgcgtcg ttaggttatt cctcattcct gcttttcgcc gctttccagg gcggccgtcc
    12721 ggtgtgagga caggatatcc gtctttagcg cttcccgcag cacttctgaa ggttggggga
    12781 actcccgcga gagaaagcgc ctttgccagc ccggcacaag ccggtccatc aggacgaaca
    12841 gcttgagccc gtgattctcc gaccaggaat cgccggcggt ttgcagcacc tgaaaaagag
    12901 tctgatgcca gggttcatcc tgcggtgcac gatctcgcgc gacctggtag tgcacccaca
    12961 ttccaagacc ttccatcgag agaaagatgt cttccagatc ggcatagaat cttctcgttc
    13021 cgctaaagaa ccgctccctg cgccgctggc cggctgacaa cgcttcggcc agcgcctcgc
    13081 ggcacaaaga gagatcatgc gcaaaaaccg cgcgggagag atggcggcgc tcctcttcgt
    13141 acatctttgc gtattcgcgg ttcttgccaa acgtgttctc gatgatatcg tcgtcgatat
    13201 cgtccgggag ttggtaccgt gtccgcaggc gtttaatgtg cttcgccgca tgtacgattt
    13261 ggcgcgtatg ggccagttca tgttgcgata ggccgagaaa caattcgtcc ggcacgggag
    13321 ccggaccggc gccggagtgt ttccggtaga acgcgggcag tggcatagta acgaacggct
    13381 tctggtcgcc tgcatacaac atcgtgaatg ctttggaggt tacagacagt gcctgtccat
    13441 cgggaatcca gaggccgccg cgatgcctca cctcgacaag cggataggtg cgccccgaga
    13501 atgtcaggaa cgaaggcacc ttgcggtgag agggtagcag ccgcttctcc ggattcaaat
    13561 gcagcgcgcg attctcgtcg tagaagatga tccatggcag cggcccggcg gaaacctgaa
    13621 ggtggcggct gcaggttgtt tcccaataat cgagtaccga ttgcatccag gccagcgtct
    13681 ggcggtcgag ttcccctacc gggggcgggg tggcaccggc aagcgtaccg caggcaagaa
    13741 tcctgcaaaa cactttgaca aagtgcgaca cggcttgcgg taattaaacc ataacaggac
    13801 ctctgtggtg ggcgtctata cttcactgcc atgccctctc cccaagcacg caagatactt
    13861 ctgtcacgaa agtggctgat tgcgttcacc gtcgtagcat tggtcctgct cactttcctc
    13921 gccgctttgt ttcacgcggc ctcttcgctt tcacgcgatt acgtggtaga ggcgctgtcg
    13981 gaaaaatacc agagcgatgt ggaactggag gagctgaacc tctctctgct tccctccatc
    14041 agggcgaccg gccataggct ctcgttccgt cacaaaggcc gaaccgacgt tccgccgctc
    14101 atcgagatac aggagttccg ggtgcaagcg ggcatcatcg gactcctgcg gagcaccaaa
    14161 cgggtatcga aagtggagct gatcggcatg aaaatcaacg ttccgcctcg ccgggaagac
    14221 aaggcggaga gcgaggagcc gaaagagaag aaatcgggcg gcattaagac acggttcgtg
    14281 atcgaagaga tcgttgccga tggcacagcg ctgcacatcc tgccgaagga ctcatggaaa
    14341 gagcctctgg ttttcgatat caagcggctt acgctgactt cagtaggtat cgaccggccc
    14401 atggagtttc gtgccggact caccaacgcc aagccgccgg gcgatatcga gagcaccgga
    14461 gagttcgggc cctgggtggc cggagacccc gggttaactc ctgtctccgg gaaatacacg
    14521 ttcgagaacg cgaacctggg ggtgttcaag ggaatcggcg gcacgctgtc ttccgtgggc
    14581 aaatataagg gagccctggc gcgcatcgaa gtggagggga ccacagacac accggatttc
    14641 gtcgtggaca ccgcgggcaa ccccgtgcat ctgaagacct cgtttcacgc cattgtggac
    14701 ggcacgaacg gagataccaa actccagccg gtgaacgcgg aatttctgaa aacgtctgtt
    14761 gttgcgcggg gaggcgtgga aggaaaggcc gggcagaagg gcaagaccgt cgcgctcgat
    14821 gttcaggtct cagaagggcg catcgaggat ctattggaac tggcggtcaa atccgacgag
    14881 ccggtcgtta ccggagccat ccgtttcacg accgcgtttg agctgccgcc gggcgaccag
    14941 gcggtgatgg acaagctcag gctgaaaggg cgcttcgggg tcgaggccgc cgagttcagc
    15001 agttccaagg tacaggagaa gatcgacgag ctcagcaaca gggcgcgggg cgagccggaa
    15061 cgcggaagca cgggcgaagt ggagtcggat ctgaaaggca atttcaccct cgcgaatggc
    15121 atgattacgc tctccggatt gtcgttctcc attccgggcg ccacggttca tctgaacggc
    15181 acttacggtc tgcgcggcga ggagataaac ttccggggta cggtccggat gcaggcgaag
    15241 gtttcggaaa cgaccaccgg tgtcaaatcg ttcttcctga aactggcaga cccgttcttc
    15301 aagaagaaag gcgccggggc ggtgataccg atcaagatcg gcgggacgcg gaagaatccg
    15361 tcgttcggac tcgatgcggg ccgcgtgatc ggtggcaagt aattttatac gggtttgccg
    15421 accgggccgg ggccgtaatc gtgcagggcc tctttgatgc tgagtccggc aatttcaatg
    15481 cgtgccaggt ctgttgtgcc gatgcctttc gcctcggcca gtttcaaggt attatcgccg
    15541 cgcacaaagg gcttggttcc ccgttcggct ttcgggtcgt agcccatcac ggccatcgat
    15601 acggcgtcga cgcacaccgg gttcttgccg cacaacagca gacccggttt gatgatttgc
    15661 actccgggat tccacgcgcc ttctccgccg cggatcgatt ccacgccgtc gacgatggaa
    15721 agatcgatgg gccgtacggc gacaaggtcg gccgttacgc gagggacgcg atagccggga
    15781 tcgcgaggag aatccggatg aagctcctgg tccactccct tgggtggcgt tttcgtaccc
    15841 gagtggcaca cgggtccgcg ctcctgttgg ggattttcat tcccctctcc tcccgcatct
    15901 ccgccgtata gggaacacgg tgtgttgccg aagttattct tcatcgccat ggtgacaccg
    15961 gcaatccagt ggttcttcag cttcgacagc gaggcataga catcgcaatc cacaaaggag
    16021 tggttcaggt gataggccgg gaaaacgtaa ccaccacccg gcactgcgat gcggacgtag
    16081 ttggcggcct gtcccttgtt ctgcacgttt tcccattcca ccttgcaccc ggcgttgttg
    16141 atggcctgga tatcgaggcc gtatcgcgcc catagatcca agtcctggcg ggccgggaaa
    16201 aagctttcga tcagcctcac acgccgcgcg ccggcgcgcg cgagaagatg caccagggcg
    16261 agcacggtat ccggattgtt acggtaggga agatccgcac gctcgggaaa gcgagtgggg
    16321 ttgcccgtga ggttcagctt caccgcgacc gtcttgcctt ttacgagcga gccgatgccg
    16381 ccgatctggt cgaacatgcg cgccatcctg gattcgaggc cgtcgctgta agtgcggcag
    16441 cgcgcaatgg cgaccgctgc cgccggggcg gaaaacgccg gctttgacag ggccaccgcc
    16501 gcgccggccg ccagcaactc acgtcgagtc atgaccgtat tcattgtcgt gttctcccct
    16561 ccaggttact tatttatgag gtgcttgtcc gcaaagttca gatattgctc ccaatcataa
    16621 tccgtcacat catgcttccc ggatcggaca tgatatccga ccgtgtccat cacggggtgg
    16681 ttcacgggcg gcatctcgtc tacccccaat ccggaagcgc caaatagctt gtagaccggc
    16741 tcggcgtacc ttgcgctcag gaactcacct ttgggatcag cccagcggtc ttcttcggcg
    16801 cttgccacat agaccgggcg gggcgcgatc agcgcgatga gcatgtgttg gtcgaaaggc
    16861 agcgcgtttt cgtttccgtt atagcgattg aaattgtccg cgaaccagtg cggaaaagct
    16921 gtattgatga tatggacggt ctctccgaat tgcctgcggc tcaaggccgc gccgccgcag
    16981 cccgagtcgt tggagatgac gagagcgaag cgcgtgtcca gagcgccagc ccaaagtgcg
    17041 gtcttgccca ggcgcgaatg gcccagtacg gccacgcggc gcgcgtcgat gtccttgtcg
    17101 gtttcaatgt aatcgagcgc ccggctcaac ccccaagccc aggcaccaat ggagccccac
    17161 tcgtcggggc caggcttggt ttgtccgggc cggtagaaca tgggttggac gccgttctgg
    17221 aacccgtcgt caaaatccgg gtcgagatcg ccgtaataga tcgtagccag gccataaccg
    17281 cgcgccagaa tcttgtcgac gggccagcgg ctttgagccg cgccccggga ttgctccgtc
    17341 gcacgatgat tcacaacgcc ggtcttgggg tttggccgca tccacgccgt cgagagtgta
    17401 atgccggcgt cgggatgaat ggcgtgattg ccctgaaagt tgagtcctat aaaaagcggc
    17461 accggcttgg cggcattctt cggcaaatag agcagcatgt ccatcttcgg cccgttcttc
    17521 tggcgcgaga aatagaccct cacctgtttg cgaatcgcac tgccgccgag cgcattgcga
    17581 tcgatggatt gcggctcgaa gtacatatcc cgcggcttgc ccgggctgta tccgaacatc
    17641 tcggattcaa acagccgcag gatttcgggg cggcgcacgt tgttccaggt ttcggcatcc
    17701 tgcaccactt cgccatccag ggtcagcagc gggtccggga ggatgtaagc cggtaccttt
    17761 gattcttcgt aattgacatc atggcgctgc gcccaggccg gccaggcgaa aaggaacagg
    17821 gcggcaggcg ctattcgaac agacattctc acttgcacac ccttaactta caggattctt
    17881 ccgcgccgtg ccgcacaggg agtaacccgt ggcgagccgc ggccggagtg gtacaaatga
    17941 ttttgaccat gatgatgcgg tgcttcctct ttctcgttcc tgcttttgct ttcatcctat
    18001 gcccgtctgc ccagccaaag gatgtcgagc ggcatcacgt cgcaaatctc gattcctccc
    18061 cacagcagta caacctgcgc cttgaaggca cggtagatgc ggccaacacg cgcgacccca
    18121 tcgtatacgc ggcatggaat caggtcttcg atcccatgcg gtccgtgaaa gtcgagaaca
    18181 cgggcgatac ggatgtaatc aacccatgga tcaccgtgaa cggcaagcgc gactggcgaa
    18241 ccaccaaagc cattgcggag gaagcgctgc gcacctatgg cgacccggcg acgatgagcg
    18301 acggcgagaa ggcgcgcgcc atctgggaat tccttcgccg caaccgcttt cacgcgacca
    18361 cgggagattt cgaagtgcgc gatcccgtga agatgttgaa tgtctacggc ttctcacttt
    18421 gcggcgacaa cgcgccggcg ttgatggacc tgtggcgctc cgccggactg aagacgcggc
    18481 gtggatttcc catcggccac tgcgtggcgg aagcctggta cgacggcgcc tggcacatgt
    18541 tggatgcgga cgagtcgatc atttttctcg atcgcgacaa tcggaccatc gcgagtgaaa
    18601 gagcggtggc tcgcgatcac gatctctcta agcgggctta tgaggacgag tatctgccga
    18661 ccctttacaa ctacgatggc acgcgctcgg gtgactaccc ggcacacacc gcgcactcga
    18721 tgaacttcac gctgcggccg ggagagtcac tggagtggcg ctgggggcag ggcagcaaac
    18781 atcactatgc gcccaacccg gtcttgtttt tgctcaagag ttcgaacctg aacgaatggg
    18841 ggccgaaggc ctgggccacg ttgcgcaatg gcaaatgggc ctacacccca ccgctgacga
    18901 aaccggcggg acggaaagga gccgaggccc ggaacatccg ttggctggaa ggcgcgggcg
    18961 ggctggcggc cattccgcaa gcggcaggcg agcttgcagc gctcacctgg gagatgcaaa
    19021 ctccgtacgt gatggtgggc ggccggctca gggcccgtac caggcttcag gcggacgact
    19081 tggttgtatt ctcgttttca caggatggca gcacgtggcg ggagatcgtc cgcaaccaat
    19141 cgcaggttga cgccagcctg gacgccttct tcccctctcc gggtgtagcg cattaccggt
    19201 atttcattcg tgcggagttc cgtgcggcga gcgatcccga ggcggtgggt ctggagtcca
    19261 ttacgatcga aaacgatctg caaatggcgc cgctggcgat gccctctctc gaactcggcg
    19321 acaatcgcat tctctatagg gacgagaccg gtgggccgcg cgcggtccgc gcaaccttcg
    19381 aatgggtgga gcgttccagc ttgaaaccgc cggacgcccc cgctacgccg gtttttccaa
    19441 aggacggcgc ggaggtagag ggcacggccg ttgtgtttga atggacgaag ccaccctcca
    19501 gcgaagagcg tcctgtagcg gactaccatt tcgaattgag cgacgaaccg gaaatgcggt
    19561 gggcgctctc gccggctttc gacgtgctga tctccaagac aaaaggcaag ggtgggacac
    19621 ggtttgccct gcccacggct gggctgctga atccaggaca gacttactac tggcgtgtgc
    19681 gggcgaaatc ggaagaaggc gtttggggcg cctggagcaa gacctggagc ttcgtaccgc
    19741 aagggccggg cattccattg ctcccgcgcc tggaggagcg cgatcccgac gcctggacgc
    19801 tcgcctggga tgccagccca cacggccgta agcctgcgcg tttccgcgtg tacgcgagcg
    19861 acgagaaagg cttcacggtc agcgaccggc cgtacgaagt cgcagtgggc aatcagaagg
    19921 aacgcggact ctacccgggg aagaagcagg taacattccc ggcgaacttc gtcgcggaga
    19981 ctggggagcc gtttcttcga atcgatccgg cccgcgccta ctaccgcgta gtcgcggtgg
    20041 acgaacgcgg taatgtgagc ggctcatccg atttcgtcga agcgccgcgg ccgtggattt
    20101 tcagccagcc ccggatggaa gcgaccatag gcgaaccata ccggtacgaa gtgaaaacca
    20161 tacggtccat cggcgatctc tcctaccgcg atttcggccc cggccaatcg taccaggcgg
    20221 cgtattggga tgcggagaag ccggagttct ccattgaaac agagatgccg cgctgcggaa
    20281 acttcgacgc gaaatggctg aagatcgatc cggataccgg ggtgttgagc ggaacgccgg
    20341 cagcggccga taccggcgaa taccagatca acgtacgggt gcgcgtgaac ggaaaagagc
    20401 acgtgcagag ttttccgctc aaagtgaacc gctgaactat gcctgcgaat acttgcggat
    20461 gatctccccg agtctgcgcg tgaggcgggg aacggctgaa acggcgcttt cccttgcttc
    20521 gtactccagc gccaggtatc cgcggtagtt ggcgttggca agcatttgca caatgcgatc
    20581 ccagtcctgc ggcccgcgcg tcccgtcttc caggcgctgt tccactttca cctgaacgtt
    20641 caccgcgtag ggaatcgaca tttcgatttg aggcagcacg ttccggttga aattgccggt
    20701 atcgaggtta atgccgagcc agggcgaatc cacctgcttc acgatctgta tgatgcgctc
    20761 cgcgcgttcg gtaatgccgc cgtggttttc aaggccgagg atcacgccgc gcgagccggc
    20821 atactcgacg cacggcttca gcgtttccac ggcccattgc gccgcctgat cttccatcga
    20881 gcctttcggc acggtgccgc cgaacacgcg gatgtgtccc gcgccgagct tggcggcgac
    20941 gtctacccac ttgcgcacct cggtgatttc cttgtcgcgc acggctccgg acgggcggca
    21001 catgtcggtg cggatggaga tgctataaat ctccaccgca ttcttgaatg cgagccgctt
    21061 caggggaaac aaaaaactgt ccggagcatc cggagggaac cagtagacgg tgaggtcgag
    21121 cccctccgct cctgtttcga cagccacccg gacgaggtcg ctgtagctca tgctctggct
    21181 cttcagctcc tcgcggtaag agtatgcgca aatggcattc cgcaggcgtg cctttccagg
    21241 tacctgttca tcggccgccc gaaggagggg agcggcggcc agcgcgggga gggaacacaa
    21301 caagtctcga cggttcatca tccgccatca tacagcagga aaaagatcag gcgcccggtt
    21361 cgaagatctt ctccgcgtat tggcggcttt ccttcaggtt gtcttcgaga gaccgtgtct
    21421 tgcgggtttc cgccacatat gcgagttcca cgaccaggta gccgcggtat gccgttgctt
    21481 tgagataatc cgccacgttc tggtaatcga tatctcccgg tccgaaggcc tcggtccaga
    21541 caccgttgac ggaattccgc aagtggaggc tcgcaaggcg gcgccgtcct tcctcgagga
    21601 tcgtcatcgg atcctgcttg ccgcggagca cccagtccac atccacgcag aaggagacgg
    21661 tcgcggagtt tgtgtttgcc agcaggtggc gccactcccg cgcgttctcg gccatttccg
    21721 gcgcgtgctg gtgcaggaag agatgcatgt ggcgtaacgc cagttcttca gcgagctggt
    21781 cgatgtagta ggcttgcgtt ttgagttcgg cgtccgactt ccgctgtcca cccggcttgg
    21841 ggcttgcgtt gaaattcaca gccgtggcgc cggcgatctt agcggcttcc gccactttta
    21901 gcgtttccgc ccaggtggct tcggccagtt cgcgcatatg catgggaccg ccgttgtaca
    21961 cgatgggcac ttcgagctta tgccgccgca gccgtctggc tgtctcatcg gccagttcag
    22021 gcttgaaaaa cgaggcatcc aactccagac gctggaatcc cgcccgtgcc gtgccggaga
    22081 atattctttc gaggttgtcg gagactggtt ttttttcggc gctcaattgc tgcatccaca
    22141 catagacctg tgaggcaagc atgggcgcat acccggtttc ctgggcaagg gcggcagccg
    22201 gcaatcccgc cagaaattcc cttctactga gggccactgt gggaactata gcatgagcaa
    22261 gctaggtgaa tggatgtgtt gaggagcgta ctcggccggt gattctgttc tgggcattgg
    22321 tgattccggc gctgttactg agcgttctcg cttacagggg cgaagcggct cgcattcgct
    22381 atatcaagcg gatcttatcg gaacctgaca ccggcgaatt tccgccggtg acggtcattg
    22441 tccccgtcaa aggagaggat gaaggtctaa gagaaaatct cgcttcgctc gccgcgctcg
    22501 attaccccga ttacgagctg attatcgcgg cgcggacgcc gggggatgtt cccggcggtg
    22561 tattgccgcc gaaggcgaaa gtggtctatg cgcgcgcggc tccgttccga gccgcgaccg
    22621 tgagggagcg ggggctgcca ggcgaggaca gctccgagaa gattggaaat ctgctggccg
    22681 ctgtcgcggc gtcgcgggcg gaatccggga tctacgcctt tgccgattcg gatggccgcg
    22741 tacgccccgg ctggcttcgc gcgctcgccg ctccgctgcg gcgaaaagaa gtgggagcgt
    22801 cgacgggtta ccgctggtac attccccgcc acccggattt ctggtcgctg ctgcgcggcg
    22861 cgtggaacgc tgtgattgcg ggctcgttcg gtcccggacc gaatggattt ctgtggggcg
    22921 gcgcgatggc gattcgcaaa caggtattcg aagaagcccg tgtggcggaa tactgggccg
    22981 gcgccctgag tgacgactat cagcttgcca gggccgtgct ggacgcagga ctcgaaatcg
    23041 catacgcgcc gggggccatg gttgcgagcg tcgatcgcat cgggccgcgc gagcttttca
    23101 gttgggtgcg gcgccagatg gtcctcacca gggtccacgc gccccggttg tggaggacgg
    23161 ctttattagc acatgtgatc tattgcggcg cgatgttggc gtgcgccgcg gccgcgttgg
    23221 cgggatggat gccggccatt ctgctgctag gcgcccaaat cggacttgga atgggaaagg
    23281 cgttggtcag gtcccgcacc gtgcgccttt gctttcccga gcacagagac tggttccggc
    23341 gatatgcgtg gatggaagtg atgctcgcgc cggccgccac ctggttgtgg ctgtgttccc
    23401 tgctggcttc tgcggcgggc aacacgattg agtggcgcgg gcgaacgtat cgtctggaac
    23461 ggccgccgag gcgcgctcgc acgactgaaa tgtgaggggc agccgccctc tgttcggcag
    23521 cgatgtcctt ctggaactgg ccaactgcat gatattatcc agaatcaggt ctgaaagtgt
    23581 tgattactct cgctcaccgt catctgcttc gtgcgctcat cccctctctg atcgtggccg
    23641 ccgccttctc gcagaccacc cctcttccct ttaagccggt tgcgttcgaa tatagcaaga
    23701 cattggatcg tctgatcctg atttcctcta acccgaaccg gctgcacatc tacgacccct
    23761 ccacatccca gacggtatct gtaacgcttg cccagccgcc attatcgctt tcggtcagcc
    23821 ccgatgggct gcgcgcggct gttgcacacg acggactgat ctcgtacgcc gatctggaaa
    23881 aaggcacgct gctgaaaacg ttcaatgttc cggcagtgat aaccggcatc gtcgtgactt
    23941 cctcttggat ctacactgtt ggtaactact atgcctccct gtcctcggtc aacatcgcta
    24001 gcggcgaggt gatccccaac agcgccgtgt tctccatgac cgccggaaag ttcaacccca
    24061 ccgtgaatgc catctatggc acgcgggacg gcacatcgcc caatgacgtc gaaagtggga
    24121 tatttcgaca ggccctgtaa aggcccaaac cgattcccct taccatggcg actactgcat
    24181 ctacggaccg gtatggtttt cgtcggattg gaaccgcatt tacaccggct gcgggaccgt
    24241 ttttcgcgct tccacggatt caaagcttga catgtattac atgtcctcca tacccggcgt
    24301 cagccagatc gggtctttgg acgagtccgc cgcgcttcag aagattgcgc tgctcctccg
    24361 cagccggtcg agctattcgg atcgggtaga tgaaagcata gtccagatct tcgagagttc
    24421 ctatctgttg ccggtgggcc agttcacgct tgccgggttt gacgctggcg gtaaaacgta
    24481 tgccccgcac ggacgagcag ttttcttccg agccgattcg accgaactta tcgtggtcaa
    24541 gcaggccgac tcctcttccg gaattctgaa cgactacgcc gtggagcgca ttccgctgag
    24601 cgccaggggg ggagtgtggc gcggcgttcg ctacgagttc gatcgacgtg ccggcggagg
    24661 ggacttccgg ttcggtgaag atcaatgccc cggcgagctg tgtataccag gcgatgagcc
    24721 tcgtgccgtg ggtcaccatt gtgtcgggtg gtcttggctc cggaaatggt acgctgcagt
    24781 gggcggtagc tgccaacgcc gattcgatct cacggaccgg cacgattatc gttccgggtc
    24841 ggatattcac tattacgcag gcggctgtgt cgtcctcgaa tccctcgtcg ctctatattc
    24901 cgggatatta cgcggtcgac gccgcctacg ccaagccaat cgataagctg gtgctcgttt
    24961 cggccaaccc caaccaactc catatcttcg atcccgcggc tcgcagggat accgtcgtgc
    25021 agctcaatgc gcccccgctg agtctttcgg tgcggccgga cggcgtccag gcggcagtcg
    25081 gccacgacgg ctgggtcacg ctagtcgacc tcaacaccgc ggcaatactg aacgtcatca
    25141 gtgtgccaac ggatgtgcac agcctcatcc tcgctggaaa cggttacgcg tacctcttcc
    25201 cccagcagga atggggcgac ctgttttcac tcgaactcag tagcggccaa attgcccgaa
    25261 cgagcgcgat ctacaacggc cgtgagccgc gccttcatgt gaacggaaag aacatatacg
    25321 tcggcgggca gtggcaatca aagtgggata tatcgggcgg ggttgcgaag ctggtgaacg
    25381 gctggccgag ctcggggtgt ggcaaactct ggctaacgga agacggacgc cgtttcttcg
    25441 gagattgcgg caaggcctat acaacgtctg aagtgccggc tcaagattat caatacaatg
    25501 ggtcgctctc gaacgcgact cggatagtct ggctcgatga atcggcgaaa gcagctacaa
    25561 tagctgccat tccgtccgcg ggtccagcct acagctccag cgcgaagtcg attgacgata
    25621 ctcaggtgca gctctatggc gacgcctatc tcggatacct cggcgcgatt cctctgccga
    25681 agtttatcgt gggaggcacg gcgttccctg ctcatgggcg ctacgtgttc tggaaccagt
    25741 ccggcaccgg gttatacgcc gtcgttcagg cggatagcaa ggcactgcta acaggcggca
    25801 ccgccatcac cgaactctca gtctccgatg cgagacaggt tgccgtgaag agtgtggtca
    25861 acagcgcgtc gcagtcggag gggcggatcg ctccgggtga actgatctcg atctacggaa
    25921 ccggcatggg gccgcaaacc ggcgcctcgt ttgcgctcga tccggtaagc ggcaaggcca
    25981 acaccagcct ggccgggacc gaagtctaca tcaacggttt ggctgccccg atcctctatg
    26041 cgtccgatac gcaagtgaac gcgatcgttc ccttcgcggc cccggtgtca ggccaggtta
    26101 ttctacaggt cggcacccaa gggattctat ccgagggtgt cacggttacg atgacggccg
    26161 ccgcgccagg cgtctttact atgaatggta gcggttccgg acaggccgtt gccgtgaatc
    26221 tcgacggcac gctctgcgat tccgctcatc ccgccgcggc cgggtcctat atcaccgtgt
    26281 actttacggg cggcggctca acgacaccag tggggtcaac gggatcggta gcagggctgg
    26341 ccctaaagcg gttatcccag actgccctcg ctacggttgc ggatataccg gcgacagtga
    26401 gtttcgccgg tgccgcgccc accctcctgg agggtgtgaa tcaactaaat ataaagctga
    26461 gcgaggagac tccaccagga ctggctcaac ctttgattgt gactgtcggg aacaacagca
    26521 gtccagcgtc tgcgacgatt gccgtcaaat gaactgatgc ccaagttggc cgccgcggcg
    26581 cgcccgcctt gccactcctg ggaccggatg ccataatcag accttaagag ggcgcggtaa
    26641 acctaatgag aacgcagtgg attgaaaagc gtaaaaacga tgctgtgcgc acgcagatgc
    26701 actatgcgcg gcgcggcatt ctgaccgagg aaatggagta cgtcgctcgc aaagagcggc
    26761 tctcgccgga agtggttcgc gaggaggttg caaaggggag gatgatcatc cccgccaaca
    26821 tcaaccaccg gaatctcgaa ccgatgtgca tcgggatcgc gtcaaaatgc aagatcaacg
    26881 cgaatatcgg aaattcggcc actacgtcga acatcgacga agaactcgag aaactccgct
    26941 acgcggtgaa gtacggctcc gatacggtaa tggatctttc aacgggcgga gacattccgg
    27001 ccatccggcg ggccatcatc gacaactctc ccgtgcccat cggcaccgtg ccgatttatg
    27061 aggcgctgac acgagtgagg cgaatcgagg atctcacgcc gcaggtgatg ctggaggtga
    27121 ttgaagaaca ggccgggcag ggcgtcgatt atatgacgat ccatgccggc gtgctcgtgc
    27181 agcacatccc tctgacaacg cggcgagtga cgggcatcgt cagccgcggc ggatcgattc
    27241 tggccgaatg gatggtcaag aatcacaagc agaactttct gtacgagcat ttcgacgaga
    27301 tttgcaaaat ctttcagaag catgacgtaa gcttttcgct cggagacggg ctgcggcccg
    27361 gttccttagc cgatgccagc gatgaagcgc agttcgcgga attgaaaacg ctcggggaat
    27421 tgacccggcg ggcctgggaa tacgatattc aggtgatgat tgaaggtcct gggcatatcc
    27481 ctatggacca aatagagtta caggttgata aagagcgcga gctgtgttac gacgcgcctt
    27541 tctacacgct ggggccgctg gtcacggatt tcgcgccggg ctacgatcac atcacctcgg
    27601 ccatcggtgc ggccatgatc ggctggtacg gtgcgtcgat gctctgttat gtgacgccca
    27661 aggagcatct gggtcttccc aaccgggagg atgtaaaggc cggcatcata gcgtataaga
    27721 ttgctgcgca cgccgccgat gtcgcgcgcc atcgccccgg ggcacgggac cgcgacgatg
    27781 aactttcccg cgcgcgctac gcattcgact ggaaccgtca gtttgaactg gcgctggatc
    27841 ccgaaacggc tcgtgccatg catgacgaga cgcttcccga agaaggcttc aaggaagcgc
    27901 acttctgttc catgtgcggc ccgaagttct gctccatgaa catttcgtcg aaggtggagg
    27961 atttcaccgc cgcggacgcg gaagcggtac tttccgggca gccgaaccgc gaactggtaa
    28021 acctgtcggg cgactaggac gccgtgtacg agctgatacg cggggcggcc ggcgaccggg
    28081 tgctttttct gtcgctgcct gaagacacag gtctgattgc gcggcttgcg ggagaagcgg
    28141 ttcggggcct tctattcgcc ctgggcgccg acgaggcggt atacgcggcg cggattgact
    28201 ttcgcgattt ccagaacgtg atgtttgcac ccggcgaacc ggacgtcatc ccttggcagg
    28261 acgccttctt cacgattgcg atcgacagtt cctgcgagtg gagggatgct gcggcaaccg
    28321 tccgcgagct ggccagagtg cttgccggcg atggtgtggc ttatctcaaa gccgatgagc
    28381 ggctgaaaac ggaactggaa gccggaggat ttctcgaagt ggattcgggc ggggaactcc
    28441 gcaagttccg gaagcttact tcctgatcag gaagaccgcg gtttccgcca tcagattcgg
    28501 ccacagagta ccggtgcggt tacccgccag gaaaatgcgg cgctccacgt tccatttttc
    28561 ctgccgtgcc aattcctcga aatcgtggac cgtgagatag tgtatgttcg g
//
LOCUS       DASTMK010000475        18260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_505179, whole genome shotgun sequence.
ACCESSION   DASTMK010000475 DASTMK010000000
VERSION     DASTMK010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18260
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505179"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..3203)
                     /locus_tag="VFQ79_19990"
     CDS             complement(39..3203)
                     /locus_tag="VFQ79_19990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU0142015.1"
                     /translation="MQIHDIGNFTAVRAAGMAKLLPYKPRIAVGMGTCGTGNGAEGVY
                     HAFAEAIDRRGLDVQLTRTGCFGFCAREPLVNVWIPGRPLVIMHQFQANQVDAVLDDL
                     AIGQITRTHALCKIEEWDHLTSQVKYGTGLPDIPLWNEIPFFKGQKKIVLRNCGLINP
                     EDIEEYIAVGGYLSLYKVLIDGTPEAVIEQIKASKLRGRGGAGYLTGIKWEYLRKAVS
                     DRKFIICNADEGDPGAYMNRNEIEGDPHSLLEGMMIGGYVMGATQGIIYVRAEYPLAV
                     HRIGRAVEQAKEYGLLGENILGRGFNFDIQLVEGAGAFVCGEETALIASLEGRAGRPR
                     PRPPFPAQKGLWGKPTNINNVETWYNIPPIVSKGPAWFTETGSVKSPGTKVFSLVGKV
                     HNTGLVEMPLGTPLKNFVYDVGEGGANGHSVKAVQTGGPSGGCIPLDMFDTPVDYETL
                     AQLGSIMGSGGMVVMDEDNCMVDVARYFIEFTHSESCGKCVPCRVGLDKALRLLNSFT
                     RGAATPAALDGLDELSRMIRDTALCGLGQTAPNPVLTTMRHFRHEFEDHIRAKRCRAG
                     VCEDLALSPCENSCPLHMNIPRFLQLYKEGRLEEAFESVILDNPLPASTGRVCQHPCD
                     NRCRRISIDEAVNMREVHRFIADSIFQSDKFDKMAELVCSKKLEPTGRKIAVVGAGPT
                     GLTAAFYLGLLGHDVTVYESAAEPGGMLRYALPDYRLPNSVLDREIELIRRLGIKFIC
                     NTPVGTDVTLNELDTQFNSVFISIGTWKEAWVYLPGTELRGVMPALVFLAGVAKKEPL
                     ELGKKVVVIGGGNAAIDSARTAIRMGSDVQIIYRRERKDMPAIREETDAAEDEGTKFV
                     FLATPHRIVGDENGRVKAIEVVKTRLGEFDSSGRRRPVPTDEIQRYACDTVILAVGEA
                     VDPDFARASGLKIKESGTVEVDRYTLETSRSKFYAGGDVITGASNVSNAMGYGKKAAK
                     NIDQRLMGVNRFDKLFRAFEYSQHPPAQISSCGRHTVREVDAAERIQNFEEAVAGFTA
                     EQTLDEATRCLRCDIRESAHEMAG"
     gene            complement(3205..3744)
                     /locus_tag="VFQ79_19995"
     CDS             complement(3205..3744)
                     /locus_tag="VFQ79_19995"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU0142016.1"
                     /translation="MSSSPTATHPEVNGRIAIDEIIAAHHGRPGGLLGILEAVQDSHP
                     AKYLPADTLEYIAAKTRIPLAQIFSVVTFYALFNLEPQGKNTICICRGTACHTRGSRN
                     LLERLKLELGIHHEAEEGQSNQSSFTTTDQKFSLRTVACFGQCALAPVVEVNHSIFGH
                     VNEQALHRELQALQQEARK"
     gene            3983..4426
                     /locus_tag="VFQ79_20000"
     CDS             3983..4426
                     /locus_tag="VFQ79_20000"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0142017.1"
                     /translation="MSSATVTFPPIATARLVVDTDVASFIFKWHPEFAPRYVGIVRGS
                     ELIVSFMTLAEMRQGALDAKWGPRRSALLEAYLADFSVLHSDSLLCSTWAAIRNESTQ
                     KGHPISSADAWIAATALVLSAPLVTNNPKDYRHLDNLQIVSAAVG"
     gene            4469..6451
                     /locus_tag="VFQ79_20005"
     CDS             4469..6451
                     /locus_tag="VFQ79_20005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0142018.1"
                     /translation="MRTPAGLSRSLIWQAGLVVLPVAILSGIALHFLREDKSSIEQEA
                     RERARALVRPLARRAGEHAGAYFGRRPVKVPLLEGVVAGGQIRSPKSYPSPPVPPDWP
                     ERLSAGQLRLWHAAQDAIYLRKDRNAARSSLASLLDPGVPREVRANAEYLLLTLGTDS
                     GRQRLMDRATRLARQSRGILAESGVPLSHLALLFALQDASPLVLGHGLEDELTHLVHE
                     HPSFLGSAVLEAAKRVAGSTSSAVAVTALEDEWQNREKALSLMRAFLKRSPSPAGPWE
                     ATVGASEELFLALGAPRESGWHVTFIPAAVAQETFRKAFDADPPLEIPAYAAAGIEIG
                     GTVWPVFGAVVRDAPLLASTTETMEVAGARHPLTLRMELTRPDLLYSPYRRRLWFVVA
                     LILSSAAAALAGLAGLWQGMRRQARVSELKSNFVSSVSHELRAPLGAIRLMAESLDAG
                     RIADPARQQEYAHLIVHECRRLSSLVENMLDFSRIDQDRRRYRFEPVDLLALVRRTLA
                     VMEPYAAEQEVSIVLREPGPELAGSSANWDVQAVEQAFVNLLDNAIKHSPAGAPVHVS
                     LESIAAGAAIRLWVGDHGEGIPKEEHARIFDLFYRHGPELRRETQGAGIGLSIVKHVA
                     EAHGGRVLVESAPGRGSRFALELPLTPPDARLKPTS"
     gene            6448..7191
                     /locus_tag="VFQ79_20010"
     CDS             6448..7191
                     /locus_tag="VFQ79_20010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0142019.1"
                     /translation="MTTAPDARILIVEDEIPMRTALADILASEGYRVLTASDGAAGLN
                     RAIEETPDLLLLDVMLPKLDGYALCAELRRLGVGIPVLMVTAKAQVHDRVTGLDSGSD
                     DYLVKPFSTDELLARVRALLRRTRRPRTAVSAIVLGDVRVDFASLTARRGSQAIRLTA
                     KEYAVLKLLAEARGEAVTREKFLDLVWKHAAFPTTRTVDNHIASLRGKLEPDAAKPRW
                     ILTVHGAGYRLEMDGAILQNADNSENHLL"
     gene            7212..7982
                     /locus_tag="VFQ79_20015"
     CDS             7212..7982
                     /locus_tag="VFQ79_20015"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142020.1"
                     /translation="MRTALVLLFLSIATDECRAQDRPAEILRQGIIAEETRQDLSAAI
                     QAYQSVLARYGEDRATAAAALFRLAEAYRKQGKTDLASAAYKRLVQEFADQREFVKLS
                     RSRLGNDVAPAGALRKYRSILEEEIRIAEQSLKLVQHEVALGAMSPSDIRQAESQLIK
                     LRTELAAFDAGLVPLQPTAPGTPEARRARDQYRKLLQVQLELADKDFAWEKKKYDLGT
                     SRRQVVIQKQIRHLELQRELAAFEAGLATQPRGEIELR"
     gene            complement(8020..10731)
                     /locus_tag="VFQ79_20020"
     CDS             complement(8020..10731)
                     /locus_tag="VFQ79_20020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142021.1"
                     /translation="MLDKVRLRLRSLFRRPNADFELEAELRFHLDRLIEENISSGMPP
                     EEARLAARRMIGGIAQYKEECRDMRRLNFVEDLVQDVRYTIRSLAKSPGFTAVVVATL
                     ALGIGANTAIFTIVHGVLLRPLDYPKPHQLMYLTAEAPAIGSTRSALSAPEYTEFRQM
                     SRSFAVVGAYSTGGAAYTTSEVNLTAGDRPLRVRSVSVDAHLLKALGIQPQQGRFFRE
                     EETTRWTGTLPPPIAILSHELWRTAFGGRPLVGQKVEIEGRLHEIVGIMPPGADVMDH
                     HTQVWLPLWLHPNMARQREAHVLYVIARRKDGVAPEVAQTELSAFLKDWGERAGTADH
                     VPTNRPLRSVDHTLQLRTLQDAIVGNASRPIWVLQAAVGFVLLIVCANLANLVMARAA
                     SRRREFVLRAALGASRRRLLRQSVTEGAVLAGAGGLLGLLLAGAGVRALIRAYPASVP
                     RTSELTIDMPVLLVALGLSTGTALLFGFVSLGRRRTSNLATALKEGAKGTTGAWRHYA
                     RRGLVIAQVAFAVMLVIGAGLLVQTVYNLTKIDAGFDRSRLVTFSMTLPMANSESDTR
                     AQAYQRVLDGLRSVPGVLGTVAMSGLPPNRTPDAIATPIENYTSGDGRPFEIIDYYQF
                     VMGDYFGTMGIPIVAGRGFERTDNASQGKVAIVNETLAKRIWKGQNPIGRRLRPPGGS
                     FGASDSVWHTVIGVARDVRQRGVERPAGTELYVSLDQHGVSPPSMNVVMRTTLPPAAL
                     SATIKRVVREVDAAVPVVRLRNMDSVFAESIRRPRLLAQLLGAFAGLALLLAAIGTYG
                     VLSYMVTERRREIGIRVALGAARSHVLTQIMKQGLQVTALGVTIGLAGALAVNRLIAS
                     LLFGVQPTDTVTIALVIATITAVAVIASWLPAWRASRLDPNVVLRDE"
     gene            complement(10740..11069)
                     /locus_tag="VFQ79_20025"
     CDS             complement(10740..11069)
                     /locus_tag="VFQ79_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0142022.1"
                     /translation="MAKPTDLVQGTLDLLILKTISLEPKHGWAIAKRIQQVSQEALQI
                     QQGSLYPALHRLEQQAWIKAEWRPTETGRMAKFYSLTRSGRKQLERELANWVRLSTAI
                     NIVVQET"
     gene            11135..13267
                     /locus_tag="VFQ79_20030"
     CDS             11135..13267
                     /locus_tag="VFQ79_20030"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0142023.1"
                     /translation="MITIKDWRMIVPEAYMRCLPILLIIPSLLCAADACRLSPEAQAD
                     LRQWLENVNHLSTAENRKGLDALVAKYPDAYEIQVRRLSFYGSILRDERPATRAAMVK
                     RAEENSKNPMALTLAGAALYRYNTPRAIELLMKARAVAPDFPWSALKLAEIYHTGRYQ
                     DLEKARTYFRTYADLCGGSISTAAERVMSKVATSGEQAEIARRIRTRLESEISPEARD
                     FEMLWALEFRTRPPQEHAALRKQVAKDVERLLALKPGKHGLGTILSGMKQSGASQEDL
                     AAFEERVLQEAPASYVAYTIAYDRWKKDHKEPESHKDTAAWDAWKAANRAAAKEWAAQ
                     FTEEPWLANSYLNVRIEAGEISKAEAVQAMDQSVNKREARSGGDLWSYINAATPLLKK
                     GWAPAKTAAWLERAWLYAEVLDRWELEDDTLTDERRKEIAGAPGYRGYLAPDLLRALY
                     AAGRKDIPASLRTYLEGAMPAKRAGHASRYRTLAWLAAVDGRTADSLAYFQQALFTRE
                     RTPQFFRGKLEDLLLDEARTAFLKTGGTEKAFAVWSRPPSGTGQLAEGRWEKPKKLLP
                     GFELADLTGKTWKLKHLEGKAVLINLWATWCGPCRSELPYFQMLYEKTKDRPDVQVIS
                     FNVDEEFGLVEPYMKEQGFTFPALAAYGLVRGMLDGYSVPQNWLVDPKGNWIATQIGF
                     AESDTDWVNSMLNRLEDAKRGKAPAATE"
     gene            complement(13284..13358)
                     /locus_tag="VFQ79_20035"
     tRNA            complement(13284..13358)
                     /locus_tag="VFQ79_20035"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13324..13326),aa:Cys,seq:gca)
     gene            13464..13871
                     /locus_tag="VFQ79_20040"
     CDS             13464..13871
                     /locus_tag="VFQ79_20040"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEU0142024.1"
                     /translation="MANLARRIFRGLAFAMALSSAIVLAEAPASKKGNTHPPRTFIAT
                     AHSQDGISASGLESEEGTVAADPDVLPLGSLIRVTGAGRYSGIYHVIDTGAKVNGRHI
                     DIYIPDSKEARRFGRRRVTVQLLKKGELIEARR"
     gene            13891..15264
                     /locus_tag="VFQ79_20045"
     CDS             13891..15264
                     /locus_tag="VFQ79_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0142025.1"
                     /translation="MATNGQQGEHEHIKEKYGLVRSLGLLQATALNMSNMVGIGPFIT
                     IPLLLASMGGPQALLGWVLGAALALCDGLIWAELAAAMPGAGGTFVYLLEAFRKTRLG
                     RVLPFLFIWQFIFSGPLEIASGYIGFAQYFGYFWRGMEPMHTRMVGLLVGVVAIILLY
                     RGITAIGKTVVVLWAGMLITVGWVIVTGLFNFDPALAFDFPPNALGFSTGFAMGLGSA
                     MLLAMYNCLGYFGICNVGGEVRNPERVIPRSIIISVIAVSSINFCITLSVIGVIPWRE
                     AINSSYVASEFMERVYGGWAGSAITVLILWSAFSSVFALLLGYSRIPYAAALDGHFFR
                     VFAKLHPRSEFPYVSLLVIGVLSAAASLLDLSSVISALMTARILIQFIGQIFAVHYIR
                     KHRPDIERPFRIWLYPIPSVIALLGWVYIFATSGVTFILYGFATVAAGLIAYAIWSRQ
                     TTGRQAI"
     gene            15424..15861
                     /locus_tag="VFQ79_20050"
     CDS             15424..15861
                     /locus_tag="VFQ79_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142026.1"
                     /translation="MGGQFMKREERRPLLTPESRKPLLEDDVRDERDKKRLTTSDIEV
                     TPEAETHIASDQTVELLPDHTLGELRARWQEVQASFVDEPRKAVRGANELVTHAIERL
                     SYSFNSERRRLEEHWEHGTDASTEDLRIALKRYRAFFERLLAM"
     gene            complement(15905..17335)
                     /locus_tag="VFQ79_20055"
     CDS             complement(15905..17335)
                     /locus_tag="VFQ79_20055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142027.1"
                     /translation="MVLNDQLRQIGRDNFDDALKITRREMIPALVTLPSVTAFYWGYH
                     RLKGEPVRAVLLGAGGQGRSHIDSINPDYIKLVAFSDIRPSAQKKARLSLQAKYGSAA
                     RDIELVEDYRKLLDRDDVEMVINALPLHLHAPVSIEAMEKGKHVLCEKLMAKTVMDCK
                     RMARAASRTGKLLSIGHQRHYSYLYANALEVTRQKDILGDVRHIRAFWHRNQTGGGKP
                     GAEKGGFDTWFINVPAEDRKIDYARHGYASIEELVRWRLFHRTGAGLMAELGSHQLDA
                     CSILLKAVLDGDAKQNRALHPAAVSGVGVNSFFTDGREVEDHVFLTYEYPRDVVVSYS
                     SITTNEMDSYGEQVMGTRGTLAILAEKDVFLFKEKSLKDTRITWAERRIAQPSAVSTS
                     TTQWTTGAGLQDTLTSRGYREEQEHLAWLIRNPGTGQPRCNPEIGLADAVVTLVSNMA
                     ASQRRRIEFKPSWFDIRSDEVPESQS"
     gene            complement(17278..>18260)
                     /locus_tag="VFQ79_20060"
     CDS             complement(17278..>18260)
                     /locus_tag="VFQ79_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142028.1"
                     /translation="LRDAVKIAAAEAIRGTAPIEDVVQFDWFYVHEEVLKVAPNPESE
                     RFSSRPYLQASAGPFRPLFRALVLDMDGLERLTPIPAQQRIDERYRQILDHFASAGKP
                     FTAAQEKKLDAARNAIKASVAAMLADPAFRTRLDDYRTMRNRAGVQASDLHVPFTRER
                     LDADRKKLDLISGELLDFVNEPLLEMAVQAETIATVDQLGAGPIPRPRDAGEWVDAFI
                     KWGLTAIGACLLLGLLTPVAALAAAAQLAIFYLASPPFPGLPAASMGGHYLYVDRNLI
                     EMIAALAVATAATGRWAGLDRYLYRLIERKLPWSSTISSAKSEETISTMP"
BASE COUNT         3549 a         5469 c         5534 g         3708 t
ORIGIN      
        1 aggcacgcta gatcgctcct tttcaccctg ctctggaatc agcccgccat ttcgtgggcg
       61 gattccctga tgtcgcaacg cagacaccgg gtcgcttcat ccagggtctg ttccgcggtg
      121 aagccggcca ccgcctcctc gaaattctga atgcgttcgg cggcatccac ttcgcggacg
      181 gtatggcggc cgcaggaaga gatttgcgcc ggcggatgtt ggctatactc gaaggcccgg
      241 aacagcttgt cgaaccggtt tacgcccatc aggcgctggt cgatattttt ggcggccttc
      301 tttccgtacc ccattgcatt cgaaacgttc gacgcgccgg tgatgacatc gccgccggca
      361 tagaatttgc tgcggctcgt ctcgagggtg tagcggtcca cctcaacggt tccggattcc
      421 ttgatcttca gtcccgaagc gcgggcgaaa tccggatcga cagcttctcc gacggccaga
      481 atcaccgtgt cgcaggcgta gcgttggatt tcatccgtgg gtacgggccg gcggcgaccg
      541 gacgaatcga actcgccgag acgcgtcttc accacctcga tggccttaac gcggccgttc
      601 tcgtctccga caatccggtg cggtgtcgcc aggaacacga acttcgtccc ctcgtcttca
      661 gccgcatcgg tttcctcgcg aatcgccggc atgtccttgc gctcgcggcg atagatgatt
      721 tgcacatccg aacccatccg gatggcggtg cgggcggaat cgatggcggc gtttccaccg
      781 ccgatgacga cgactttttt acccagttcg agcggctcct tcttggccac gcccgccagg
      841 aagaccaggg cgggcatgac gccgcgaagt tccgtgccgg gcaggtaaac ccacgcttcc
      901 ttccaggtgc cgatggagat gaagacgctg ttgaactgcg tatccagttc gttgagcgtt
      961 acgtcggtac ccaccggcgt gttgcagatg aacttgatgc cgaggcgccg gatcagctcg
     1021 atctcacgat cgagcacgga gttcggcagc cggtagtccg gcaaggcgta gcgaagcata
     1081 ccgccgggct cggccgccga ttcgtagacc gtcacgtcat gccccagcag gccgagatag
     1141 aaagcggcgg tcaatcccgt gggaccggct ccgacgacgg cgatcttccg tccggtcggt
     1201 tccagtttct tcgaacaaac cagttccgcc atcttgtcga acttgtcgga ttgaaaaatg
     1261 gaatccgcga taaagcgatg gacctcgcgc atgttgacgg cttcgtcgat actgatgcgg
     1321 cggcagcggt tgtcgcacgg atgctggcag acccttccgg tggaagccgg caatgggttg
     1381 tccaggatca cggattcgaa ggcctcctcc agccggccct ctttataaag ctgaaggaaa
     1441 cgcgggatgt tcatgtgcag cggacaactg ttctcgcacg gggaaagcgc gaggtcctcg
     1501 cacacgcccg cgcggcagcg tttcgcgcga atgtgatcct cgaattcgtg ccggaagtgg
     1561 cgcatcgtgg tgagcacggg attgggcgcg gtttggccga gcccgcagag agcggtatcc
     1621 cggatcatcc ggctcagctc gtcgagaccg tcgagcgcgg ctggtgtggc ggcgccgcgc
     1681 gtaaaggaat tcagcagccg cagcgccttg tccagcccta cgcggcacgg cacacatttt
     1741 ccgcaggatt ccgagtgggt gaactcgatg aagtaacggg ccacgtcgac catgcagttg
     1801 tcttcatcca tgaccaccat tccaccggag cccatgatgg agccaagctg cgcgagagtc
     1861 tcgtaatcca ccggcgtgtc aaacatatcg agtggaatgc agccgccaga tggtcctccc
     1921 gtctgcacgg ccttcaccga gtggccgttc gcgccccctt ctccaacgtc gtagacaaag
     1981 tttttgagcg gagtgcccaa cggcatttca acgagccctg tgttgtggac cttgccgaca
     2041 agagaaaaga cttttgttcc cgggcttttc acgctcccgg tttccgtaaa ccacgccgga
     2101 cctttcgaca caataggcgg tatgttgtac caggtctcaa cgttattgat gttcgtcggt
     2161 ttgccccaca gacccttctg cgcgggaaat ggcgggcggg gccggggccg tccggcgcgg
     2221 ccttcgagtg aggcgatcag cgcggtctct tccccgcaga caaaggcgcc cgcgccttcg
     2281 acaagctgta tgtcgaaatt gaacccgcgc ccgagaatgt tctcgccgag cagaccgtat
     2341 tcttttgcct gctctacagc cctgccgatg cgatgcactg ccaacgggta ttcggcgcga
     2401 acgtagatga tgccctgcgt cgcccccatg acataaccgc cgatcatcat tccttccagc
     2461 agcgagtgcg gatcaccctc tatctcgttg cggttcatgt acgcgccggg gtcgccttca
     2521 tcggcgttgc agatgataaa tttccggtcc gacaccgctt tccgcaggta ttcccatttg
     2581 atgccggtca ggtatccggc tccgccgcgg cctctcagtt tcgaggcctt gatctgctcg
     2641 attacggcct cgggtgtgcc gtcgatgaga actttataga gcgacaaata cccgccgacg
     2701 gcaatgtatt cctcgatgtc ttcgggatta atgaggccac agttgcgaag gacaattttc
     2761 ttctgtcctt taaagaaagg gatctcgttc cacaaaggaa tgtccggcaa tccggtgccg
     2821 tatttgacct ggctggtaag gtggtcccac tcctcgattt tgcagagcgc gtgggtccgt
     2881 gtgatctgcc cgattgcaag atcgtccagg acggcgtcta cctggttggc ctgaaactgg
     2941 tgcatgatca ccagcgggcg tccggggatc cagacgttta ccagcggctc gcgcgcgcag
     3001 aacccgaagc agcctgtgcg ggtgagttgg acgtccagcc cgcgccggtc gatggcctcc
     3061 gcgaacgcgt ggtacactcc ttcggcgccg ttgcctgttc cgcacgttcc catgccgacg
     3121 gcaattcgcg gcttataggg gagaagcttc gccatgccgg ccgcgcggac tgcggtgaag
     3181 ttgccgatgt catgaatctg catgttactt gcgggcctcc tgctgcaatg cctggagttc
     3241 gcggtgcaac gcctgttcat tgacgtgccc gaagatggag tgattgacct ccacaaccgg
     3301 agcgagcgca cactggccga agcaggccac cgttctcaac gaaaacttct ggtcggtggt
     3361 tgtgaagctc gactggttcg actggccctc ctcggcctcg tgatgtatgc cgagttccag
     3421 cttcaagcgt tcgagcaggt tgcgggagcc gcgcgtatgg caggcggttc cacggcaaat
     3481 gcagatggtg tttttcccct gaggttcgag attgaagagt gcgtaaaaag ttacgacgct
     3541 aaagatttgc gccaggggaa tccgcgtttt ggcggcaata tactccagcg tgtcggcggg
     3601 caggtatttg gccggatgag aatcctgcac cgcctccaga atgccgagaa gtccgccggg
     3661 ccgtccatga tgcgcggcaa taatttcatc gatagcaatt cgcccgttta cttccgggtg
     3721 agtggctgtt ggtgagctgc tcattgtggc tatgctccct ttatttcagt gcaacagatc
     3781 atattatctt aatttgtcaa tccatgaaac cggtaatggc tacggagcgc taaaataatg
     3841 gtcgagatgg atcatgtgcc aggacaaccc gtaaaagatc cgcccaacct ggtccgggct
     3901 ggcgcgtttt cgctggatcg tctgttgagc catattgacc caggaacagc ggaggaatcc
     3961 gaggagttcg tacgccgcat ctatgagcag cgccacagtg acctttcctc cgatcgcaac
     4021 ggcaagactg gtcgttgaca cagacgtcgc cagctttatc ttcaagtggc atcccgaatt
     4081 cgcgccacgc tatgtgggaa ttgtgcgcgg ttccgagttg attgtttcat tcatgactct
     4141 ggccgagatg cgccagggtg ccctggatgc gaagtgggga ccccgcagga gcgctttgct
     4201 ggaagcctat ttggccgact tctcagtcct tcattcagac agcctgctgt gttccacgtg
     4261 ggccgccatt cggaacgaga gcacgcaaaa gggacatccc attagttcgg ccgatgcgtg
     4321 gattgcagcc acggcgttgg tcctctcggc gccactggtg acgaacaacc ccaaggacta
     4381 tcgccattta gacaaccttc agattgtttc tgcggcggtc ggataaccct agcgctgcgc
     4441 tacagtagca tttcagggct tcatcgctat gaggactccc gccgggctta gccgttcttt
     4501 gatctggcag gctgggcttg tcgtccttcc ggtagcgatc ctctccggga ttgccctcca
     4561 tttcctgcgc gaggacaagt cttcgataga acaagaggcc cgcgagcgcg cacgtgccct
     4621 cgtgcggccg ttggccagaa gagccggtga gcatgcggga gcctatttcg ggcggcgacc
     4681 ggtcaaggtg cctctgctcg aaggagtcgt cgccggtggg cagattcgct cgccaaaatc
     4741 ttacccttcc ccgccggtgc cgccggactg gcctgaacga ttgtccgccg gccaactccg
     4801 cctctggcac gccgctcaag atgctatcta cctacgaaag gaccggaacg ctgcccggag
     4861 ttctttggcc tcgctgctgg atcccggcgt gccgcgggag gtgcgtgcta atgcagagta
     4921 tctccttctt acacttggca ccgattccgg ccgtcagcgc ctgatggatc gggccacaag
     4981 actggcccgg cagtcgcgag gaatccttgc cgaatcgggt gtcccgctgt cacatcttgc
     5041 gcttctgttc gccctgcaag acgcgtcacc gctggttttg ggtcacggat tagaagacga
     5101 gttgactcat ctcgttcacg agcacccatc gttcctgggc tctgcagtat tggaagctgc
     5161 caagcgggtt gctggtagta cgtcatcggc cgtagcggtc actgcgctcg aagacgagtg
     5221 gcagaatcgc gagaaagccc tctccttgat gcgtgctttc ctcaagcgct cgccctcacc
     5281 ggcaggccct tgggaggcga ccgtcggagc atccgaggag ctctttttgg ctctgggtgc
     5341 gccgcgtgag tcaggatggc atgtaacttt catccccgcc gcggtcgcgc aagaaacttt
     5401 ccggaaggcc ttcgacgcgg atccaccgct cgaaatcccc gcctatgctg cggctggtat
     5461 cgaaatcggt gggacggttt ggccggtttt tggagcggtg gtccgcgacg caccgctatt
     5521 agcctccact acggaaacca tggaggttgc gggcgcgcgc catccactca cactccgcat
     5581 ggaactgacc cggccggacc tgctctactc tccctatcgg cggcgcctgt ggttcgtcgt
     5641 cgctttaatt ctgtccagtg ccgccgctgc tttggccgga ctcgccggcc tttggcaggg
     5701 aatgcgccgc caggcgaggg tgagcgagtt gaagtccaac ttcgtctcca gcgtgtcgca
     5761 cgaattgcgc gcgccactgg gtgccattcg gctcatggcc gaaagtctgg atgcaggcag
     5821 aatcgccgat ccagccaggc agcaggagta cgctcatctc atcgttcatg agtgccgccg
     5881 cctctcatcc ctggtcgaga acatgctgga cttctcgcgt atcgatcagg accgccggcg
     5941 gtaccgtttt gaaccggtag acttgctggc attggtacgc cggacccttg ccgtaatgga
     6001 accctatgcg gccgaacagg aggtcagcat cgtgttgcgc gaacccgggc ccgaactggc
     6061 cggctcctca gcgaattggg atgttcaggc cgtggaacag gccttcgtaa acctgctgga
     6121 caacgccatc aagcattcgc cggccggagc gcctgttcac gtctcactcg aatctatagc
     6181 ggcgggcgcc gctatccgcc tctgggtggg ggatcacggc gagggcatcc cgaaagagga
     6241 gcatgccagg atcttcgatc tcttctaccg gcacggcccg gaattacgcc gcgaaactca
     6301 gggcgctgga attggattga gtatcgtgaa acatgtggcg gaagcgcatg gaggtcgcgt
     6361 gctggtagag agcgcgcccg gccgcggcag ccgattcgcg ttggaattgc ctctaacgcc
     6421 ccctgatgcg cgcctgaaac ccacgtcatg accaccgcac cggatgctcg catactcatc
     6481 gttgaagacg agatacccat gcgcaccgcg ctggccgata ttctggcatc ggagggctac
     6541 cgcgtcctga ccgcctccga cggagctgcc gggctcaacc gggctatcga agagacgccg
     6601 gacctcttgc tcctcgatgt catgctcccg aagctggacg gttatgcttt atgcgccgag
     6661 ttgcggcgcc tgggcgtcgg catacccgtg ttgatggtta cagcgaaagc ccaggtccac
     6721 gaccgggtca ccgggctcga cagcggctcc gatgattatc tcgtgaagcc gttcagcacg
     6781 gatgagttgc tcgcccgggt gcgggctctg ctgcggcgaa cacgacgccc tcgcacggcg
     6841 gtatcggcaa tcgtcctggg cgacgtacgc gttgatttcg ccagcctgac cgcccgccgc
     6901 ggcagtcagg caatccgcct gactgcaaaa gaatatgccg tcttgaagct gctggccgag
     6961 gcgcggggtg aggccgtaac gcgcgagaag tttctcgacc ttgtctggaa gcatgccgcc
     7021 ttccctacca cgcgcaccgt agacaatcac atcgccagtc tcagggggaa gctggagccg
     7081 gacgcggcca agccccgctg gatcctcacc gtgcacggcg ccggataccg gctcgaaatg
     7141 gatggagcga ttttgcaaaa tgctgacaat tcggagaatc acctcctata aagtattcct
     7201 gggagccaat catgagaacc gccctggtgt tgttgttcct atccatagct acagatgagt
     7261 gccgcgcgca ggaccgcccg gccgaaatac ttcgccaggg catcatcgcg gaggaaacca
     7321 ggcaggacct gagtgcggca attcaggctt accaatccgt gctcgcgcga tatggcgaag
     7381 accgcgccac ggccgcggcg gcacttttcc ggctggctga ggcttatcgc aagcagggga
     7441 aaacggatct cgcgtccgcg gcgtacaagc gcctcgtcca ggaatttgcc gaccagcgtg
     7501 agtttgtcaa gcttagtcga agccgcctgg gaaatgacgt tgcgccggct ggggccttgc
     7561 gcaaataccg gtcgatcctg gaagaagaaa tccgtattgc agagcaatct ctgaagctcg
     7621 ttcaacacga agtcgcactg ggcgccatgt caccttcgga tatccgtcaa gcggaatcgc
     7681 agctcattaa actgcggacg gaattggcag cctttgatgc cggcctggtt ccactacagc
     7741 caacggctcc cggcaccccc gaagccaggc gcgcgcgaga tcaatacagg aagcttttgc
     7801 aagtccaatt ggagcttgca gacaaagatt tcgcgtggga aaagaagaag tacgatttag
     7861 gaacaagcag gcggcaggtc gtgatccaga agcaaatcag gcatctcgaa ctccagcgcg
     7921 aactggcggc gttcgaagcg ggattagcca cccagccgcg cggcgaaatc gagctgcgat
     7981 gacgtaaaat cccgctcgcc agggttttct ccgtttctgt tactcatctc tgagcacgac
     8041 gttcgggtcc agccgcgatg cgcgccacgc cggcagccaa ctggcgatca ccgcgacggc
     8101 cgtaatcgtt gcgatcacaa gggcaatcgt cacggtatct gtcggctgca cgccaaacag
     8161 cagggacgca atcagccgat ttacggcaag cgcgcctgca aggccaatgg tgacacccaa
     8221 cgccgtgacc tgaagccctt gcttcatgat ctgtgtgagc acgtgggagc gagctgcacc
     8281 gagggcgacc cgaataccga tctcgcgccg gcgctccgtc accatgtagg agagcacacc
     8341 gtaagtgccg atggctgcca gtagcagcgc cagtccggca aaagcgccca atagctgcgc
     8401 caggagtctc ggccggcgaa ttgattcggc gaacaccgag tccatgttgc gaagccgcac
     8461 gacgggaacc gcggcgtcca cttcccgaac cactcgcttg atcgtcgctg acagcgcggc
     8521 cggtggcagc gtcgtccgca tcaccacatt catactcggc ggagagacgc cgtgctggtc
     8581 aagggatacg taaagctcag ttcctgccgg acgctccacc ccgcgctgcc tgacgtccct
     8641 ggctactccg atcaccgtat gccagacact atcgctggcg ccaaacgaac cacccggagg
     8701 ccgcagacgc cggccaatcg ggttctgccc cttccagatc cttttcgcca aagtctcatt
     8761 gacgatggcc accttgcctt gcgacgcgtt gtcggttcgc tcgaaaccac ggccggcgac
     8821 gatcgggatg cccatcgtcc cgaaatagtc gcccatgacg aattggtagt agtcgatgat
     8881 ttcgaagggc cttccatcgc cggaggtgta gttctcgatc ggagtagcga tcgcatcggg
     8941 agttcgattc ggtggtagac ccgacatggc aaccgttccc agcacgcccg gtacggagcg
     9001 aagcccgtca aggacgcgct gatatgcctg cgcgcgtgtg tcggattctg aattcgccat
     9061 gggcaatgtc atcgagaacg ttaccagccg cgaccggtca aatcctgcat cgatcttcgt
     9121 gaggttgtag actgtctgaa ccagcaaacc cgctccgata accagcatca ccgcgaaagc
     9181 aacctgggct atcacaagcc cacggcgggc gtaatgacgc caggcgccgg tggtcccttt
     9241 tgcgccctcc ttgagcgcag tcgccaagtt gctcgttcgc cggcgcccga gcgacacgaa
     9301 tccgaaaaga agggccgtcc cagtcgagag gccgagcgca accagcagaa cgggcatatc
     9361 gatcgtaagc tcacttgtcc tcgggacgct ggccgggtaa gcacggatca gcgcccgcac
     9421 gccagcgccg gctagcaaca gacccagtag gccaccggct ccggccagca ccgcaccttc
     9481 ggttaccgac tgccggagca gccggcggcg gctggcgccg agagcagcgc gcagcacaaa
     9541 ttcccgtctg cgtgaggcgg cgcgagccat caccaggttt gcaagattcg cgcagacgat
     9601 gagcagcacg aatcccacgg cggcctgcag aacccagatc gggcggctgg cattcccaac
     9661 gatcgcgtct tgtaacgtcc gcaattggag ggtgtggtct actgagcgca ggggacggtt
     9721 ggtggggacg tggtctgccg tgccggcacg ctcgccccag tctttgagga atgcgctcaa
     9781 ctccgtctgc gccacctcag gggcgacgcc gtctttccgt cgcgcaatga cgtagaggac
     9841 gtgggcctcg cgttgccggg ccatattcgg gtggagccac aggggcaacc aaacctgggt
     9901 gtggtgatcc atgacgtcgg ccccgggcgg cataatgccc acgatttcgt ggagccggcc
     9961 ttcgatttcc accttctgtc caacgagcgg ccgccctcca aacgctgttc gccacaattc
    10021 gtgcgacaga atcgcgatcg gtggcggcag cgtcccggtc caacgcgtgg tttcttcttc
    10081 acggaagaac cgtccctgct gcggctgaat accgagcgcc ttgagcagat gtgcatcgac
    10141 ggagaccgag cgcacccgca gaggccggtc ccctgccgtg aggttgacct cactggttgt
    10201 atacgccgcg ccaccggtcg aataagcgcc aaccaccgcg aacgaccggc tcatctggcg
    10261 gaactccgta tactctggcg cagagagcgc acttcgggtg cttccaatgg ccggagcttc
    10321 cgccgtcaga tacatcagtt gatggggttt tggatagtcg aggggccgca gcagcacccc
    10381 atgtacgatc gtaaagatgg cagtatttgc tccaatccca agcgccagcg tggcgacgac
    10441 aactgctgtg aacccaggac tcttcgctaa agaccggatc gtgtaacgta cgtcttgtac
    10501 aagatcctcg acaaagttta ggcggcgcat atcccggcac tcctccttgt attgcgcgat
    10561 gcccccgatc atgcgccgtg cggccaagcg ggcctcctca ggcggcatac cggatgaaat
    10621 gttctcttca atgagccgat cgaggtggaa acgaagttcg gcctcgagtt cgaagtcggc
    10681 atttggacgg cggaacagcg atcggagccg caaacggact ttgtctaaca tccgcattac
    10741 taagtctcct ggaccacgat attgatggcc gtcgacagac gaacccagtt cgccaattcc
    10801 ctttccagtt gtttccgccc cgaacgtgta agggagtaga actttgccat tcgcccggtc
    10861 tcggtggggc gccactcggc cttgatccag gcctgctgct ccagacggtg cagcgccggg
    10921 tacagggacc cctgctggat ctggagagct tcctgtgaaa cctgctggat gcgcttggcg
    10981 atggcccagc cgtgtttagg ttcgagcgag atggtcttga ggatcaatag atcgagagtg
    11041 ccctggacaa gatcggtcgg ctttgccata tgcctagatt actaacaatt gtagacgatc
    11101 acaccttcct tgccgaactc cctggctatg cctcatgatt actatcaaag attggcgtat
    11161 gatcgtcccg gaggcgtata tgcgctgtct gcccatcctg ctgatcatcc cttctcttct
    11221 gtgcgccgcg gatgcgtgcc ggttgtcccc cgaggcgcaa gcggacctgc ggcagtggct
    11281 ggagaacgtg aaccatttgt cgacggccga aaaccgcaag ggtctcgatg cacttgtcgc
    11341 gaagtatccc gacgcatacg aaattcaggt gcggcgtctc tctttctacg ggtctatact
    11401 tcgcgatgaa cggcctgcca cacgtgcggc aatggtgaag cgcgccgaag agaacagtaa
    11461 aaatccgatg gccctgacgc tcgctggggc cgctctgtac cggtacaaca caccccgtgc
    11521 gatcgaacta ctgatgaaag cacgtgcggt cgcgcccgac tttccgtggt cggcgttgaa
    11581 actcgccgag atctaccata ccggtcgtta ccaggacctc gagaaagccc gcacatactt
    11641 ccgaacgtat gccgacctct gcggtggcag catttcaacc gcagcagaac gggtgatgag
    11701 caaggttgct acatccggcg aacaggcgga gatcgcccgg cgcattcgga ctcgactgga
    11761 gtctgagatc tcgccggagg cccgcgattt tgaaatgctt tgggcgctgg agttccgtac
    11821 tcgcccgccg caggaacacg ccgcgttgcg gaagcaggtg gcaaaggatg tcgaacgcct
    11881 gctcgcgttg aagcccggca agcatggtct cgggacgata ctcagcggca tgaagcagag
    11941 cggtgcttcc caggaggacc tcgccgcgtt cgaggagcgc gtgcttcaag aagctccggc
    12001 gtcgtatgtg gcctacacta tcgcgtacga ccgctggaag aaagaccaca aagaaccgga
    12061 gagccacaag gacaccgccg cctgggatgc gtggaaagcg gcgaaccgcg cggcggcgaa
    12121 agaatgggcc gcccaattca ccgaggagcc ctggctcgcc aattcgtacc tcaatgtgcg
    12181 gatcgaggca ggtgagatca gtaaggcgga agcagtgcag gccatggacc aatccgtcaa
    12241 caagcgcgag gctcgttccg gcggtgacct gtggtcctac atcaacgccg cgacgccgct
    12301 gttgaagaaa ggctgggctc cggccaaaac ggcggcgtgg ctcgaaagag cgtggctcta
    12361 cgcagaagtg ctcgaccggt gggagcttga agacgacaca ctcactgacg agcggcggaa
    12421 ggagattgct ggggcgcccg ggtatcgcgg ctatctcgca cccgatctgc tgcgagcgct
    12481 ctacgccgct gggcgcaagg acattcctgc atcgctgcgc acctatctcg aaggtgcgat
    12541 gccggcgaag agagccggcc acgcttcgcg ctatcgcacg ctcgcctggc tggccgctgt
    12601 ggacggtcgt actgccgatt cgctcgcgta ttttcagcag gcgcttttca ctcgcgagag
    12661 gacaccgcag tttttccggg gcaaactcga ggatttacta ctcgatgaag cccgaactgc
    12721 cttcctgaag accggcggca ccgaaaaagc gtttgccgta tggtcccggc cgccgtcagg
    12781 taccgggcag cttgccgaag gccgttggga gaagcccaag aagctactac cgggcttcga
    12841 gttggctgac ctcacgggaa agacttggaa gctgaagcac cttgaaggca aagcggtcct
    12901 cattaacctc tgggcgacct ggtgcggacc ctgccgctcc gagcttcctt acttccagat
    12961 gctgtacgag aagacaaagg accgcccgga cgttcaggtg atttcgttta acgtggacga
    13021 ggagttcgga ctcgttgagc cgtatatgaa agagcagggg tttacgttcc ccgcgctcgc
    13081 ggcatacggg ttggtccgcg gcatgcttga cggctacagc gttccacaga actggcttgt
    13141 ggacccgaag gggaactgga tcgcaacgca aatcggcttc gctgagagcg acaccgattg
    13201 ggtcaactcc atgctgaatc ggctggagga tgcaaagcga gggaaggctc cggcagctac
    13261 cgagtagcta ccaggtggga agctggaggc gcgggcggga atcgaacccg cgaataaagg
    13321 ttttgcagac ctctgcctta ccacttggct accgcgccgt atcggagagt ctcttctaaa
    13381 gttagcgccc tgacgtattc ttcgtcaatc atcccacccc tttgaatttt gtaccgggac
    13441 aggaaagagg ttaggatggg cgaatggcga atttggcacg acggatcttc cgggggcttg
    13501 cattcgctat ggccctaagc tccgcgatcg tactcgcgga agctcctgcc tcgaagaagg
    13561 gaaacacgca cccgccgcgg actttcatcg caaccgcaca ttcgcaggac ggcatctcgg
    13621 cgtccggact cgaatcggag gaaggcactg tagcggccga tcccgatgtg cttccgcttg
    13681 gttccctcat ccgcgtgacc ggtgcgggcc gctattccgg catctatcat gtcatcgata
    13741 caggcgcgaa agtgaacggc cgccacatcg atatctatat ccccgattcg aaagaagcgc
    13801 ggcgcttcgg ccggcggcgt gttaccgtcc agttgttgaa gaagggtgaa ctgatagaag
    13861 cgcgccgtta acacggagga actcgacagc ttggctacca acggacagca gggcgagcac
    13921 gagcatatca aagagaaata tggactggtc cggagtctgg gcttactaca ggcgacggcg
    13981 ctgaacatga gcaacatggt ggggatcggg ccgttcatta cgatcccgct gctgctggcc
    14041 tcgatgggcg ggccgcaggc gctgttgggc tgggtgctcg gcgcggccct ggcgctgtgc
    14101 gacggtctca tctgggccga actcgcggcg gcgatgccgg gcgcgggcgg caccttcgtc
    14161 tacctgctgg aggcgttccg gaagaccagg ctgggacgtg ttctgccgtt tctctttatc
    14221 tggcagttca tcttcagcgg accgctggag atcgcttccg gctacatcgg gtttgcccaa
    14281 tatttcggtt acttctggcg tgggatggag cccatgcaca ccagaatggt cggtctgttg
    14341 gtaggagtgg tggccatcat tctgctttat cgcggcatta ccgcaatcgg aaaaacagtc
    14401 gtggtgttgt gggcgggaat gctcatcacc gtgggatggg tgatcgtcac cggattgttt
    14461 aatttcgatc cggcgctggc attcgatttt cccccgaacg ctctcggctt ttcgaccggg
    14521 tttgcgatgg gtctgggctc cgccatgctc ctcgccatgt ataactgcct gggctacttc
    14581 ggaatctgca acgtcggcgg tgaggttcga aatccggaac gcgtgatacc gcgctctatc
    14641 atcatttccg tgatcgcggt gtcatcgatc aacttctgca tcaccctgag cgtaatcggc
    14701 gtgatcccct ggcgggaggc gatcaactcc agctatgtgg cctccgagtt catggagcgt
    14761 gtttatggcg gttgggcagg cagcgccatc acggtcctga ttctatggtc cgcgttctcc
    14821 tccgtattcg ctttgctgct gggctatagc agaattccgt atgctgccgc actcgacggc
    14881 catttcttcc gcgtgtttgc gaaactgcac ccgcgtagcg agttccccta tgtttcgttg
    14941 ctcgtcattg gcgttctgtc agccgccgca agcctgttgg acttaagctc cgtgatctcc
    15001 gcgttgatga cggcgcgtat tttgattcag ttcatcggcc agattttcgc cgttcactac
    15061 atccggaaac acaggccgga tatcgaacgc ccgttccgga tctggctgta tcctattccg
    15121 agtgtcatcg ctctactcgg ttgggtctac atcttcgcga catccggcgt cacattcatc
    15181 ctgtatgggt tcgctaccgt ggcggcgggt ttgattgcct atgcgatctg gtcacggcag
    15241 accacggggc ggcaagcgat ctaaactccg aggctccgcc cgcctcccgt tccaggaaaa
    15301 tacttcaggg atgcctgaaa gccgagaggt ccgtccggcg ctcgaaatcg ttcggaatga
    15361 ggcgggtaaa cagggtgccg tggctggcag cataaatgca attacaacgg tgagggagga
    15421 cctatgggtg gacaatttat gaagcgtgaa gaacgtagac cgctccttac acccgaaagt
    15481 cgaaagccgt tgctggagga cgatgtccgg gacgaacggg acaagaagcg tcttacgact
    15541 tccgatattg aagtcacgcc cgaggcggag acacacatag caagtgatca gacagtggaa
    15601 ttgctgccgg atcatacgct cggcgagctc cgcgcccgct ggcaggaagt tcaggccagc
    15661 tttgtcgacg agccgcgcaa agccgtccgg ggtgcaaatg aactggtgac ccacgctatc
    15721 gagcggctca gttactcgtt taacagcgag cgcaggcggc tcgaggaaca ttgggaacac
    15781 ggaacggatg catcgacgga agatttgcgc atcgccttga agcgctatcg ggcattcttt
    15841 gagcgtctcc tggcgatgtg aggttcgccg cgtcaacatg gcggccagcc ggcgccaccg
    15901 cggtttaaga ctggctctcc ggcacctcgt cggagcgaat gtcaaaccag gatggcttaa
    15961 attcgatacg gcggcgttga ctggccgcca tgtttgacac cagggtgacg acggcatcgg
    16021 ccagtccgat ttcggggtta catcgcggtt ggcctgttcc cggattgcgg atcaaccatg
    16081 ccaggtgttc ctgctcctcg cggtacccgc ggctggtaag cgtatcctgc aatccggctc
    16141 ccgtggtcca ctgggtggta gacgtcgaga ccgcggacgg ctgcgcaatg cggcgctcgg
    16201 cccacgtaat gcgagtgtcc ttgagcgact tctccttgaa caggaagaca tccttttccg
    16261 ccagaatcgc taaagtaccg cgcgtgccca tcacctgctc tccgtaactg tccatctcgt
    16321 tggtagtaat actggagtaa cttactacta cgtctcgcgg atactcgtaa gtcaggaaaa
    16381 catggtcctc cacttcacgg ccatcggtga agaacgagtt cacgccaaca cccgaaacgg
    16441 ccgccggatg cagagcgcga ttttgcttcg cgtccccatc cagaacggcc ttgagcagga
    16501 tgctgcatgc atcgagctga tggctgccga gttcggccat aaggcccgca cccgtgcgat
    16561 ggaaaagacg ccagcgcacc agctcttcga tactcgcata accatgcctg gcatagtcga
    16621 tcttccggtc ctccgccggc acgttgatga accacgtatc gaagccgcct ttctcggcgc
    16681 cgggttttcc tcctccggtc tggttgcgat gccaaaatgc gcggatgtgg cgaacatctc
    16741 cgaggatatc tttctgccgt gtgacctcga gagcgttggc gtagagatag ctgtagtgcc
    16801 gctgatggcc gatggaaagc agcttgcccg ttctggacgc ggcgcgggcc atgcgcttgc
    16861 agtccatcac tgttttcgcc atcagctttt cgcaaaggac gtgcttgcct ttctccatcg
    16921 cctcgatact gacgggggcg tggagatgca gcggcagggc gttgataacc atctcgacat
    16981 cgtcgcgatc gagcagcttg cggtaatctt cgaccagttc gatgtcgcgc gcggcgctgc
    17041 cgtacttggc ctggagcgac aggcgggcct tcttttgcgc gcttggacgg atgtcagaga
    17101 acgcgacaag cttaatgtag tcgggattga tggagtcgat gtgcgagcgc ccctggccgc
    17161 ccgcgccaag cagcaccgcg cgtaccggct cgcccttcag ccggtggtag ccccagtaaa
    17221 aagccgtgac cgagggcagc gtaaccaggg ccgggatcat ctcgcggcgc gtaattttca
    17281 gggcatcgtc gaaattgtct cttccgattt ggcggagctg atcgttgagg accatggcag
    17341 tttcctttca atgagacggt acaggtaccg gtcgagcccg gcccagcggc cggtggccgc
    17401 cgtcgcgacg gccaaagcgg caatcatctc gatcagattg cgatcgacgt acaggtagtg
    17461 cccacccatg gatgccgccg gcagacccgg gaatggagga gaggcaagat agaagatcgc
    17521 gagttgcgcg gcggctgcga gggctgccac cggggtgagc agtcccagca gaaggcaggc
    17581 gccgatagcc gtcaagcccc actttatgaa tgcgtccacc cattcgccgg catcgcgcgg
    17641 gcggggaatc ggaccggctc cgagttgatc caccgtcgcg atggtctccg cttgtaccgc
    17701 catctccaat aggggttcgt tcacgaagtc cagcaactcg ccggagatca ggtcgagctt
    17761 tttgcgatcc gcatcgaggc gctcgcgggt gaacggcaca tgcaagtccg aagcctggac
    17821 gccggcgcgg ttccgcatcg tccggtagtc gtcaagccgg gtacgaaagg caggatcggc
    17881 cagcatggcc gcgaccgatg ctttgatcgc gttgcgcgcg gcatccagct ttttttcctg
    17941 cgcggcggtg aagggtttgc cggcggaagc aaaatgatcg aggatctggc gatagcgctc
    18001 gtcaatgcgc tgctgagccg ggatgggcgt cagacgttcc agtccatcca tgtcgaggac
    18061 cagcgcacga aacagcggcc ggaaggggcc ggccgaggcc tgtaaataag ggcgcgagga
    18121 gaagcgttcg ctttccgggt ttggcgccac cttcagcacc tcttcatgaa catagaacca
    18181 gtcgaattgg acgacatctt cgataggtgc tgttccccga atggcttctg cggccgcgat
    18241 cttcaccgcg tcgcgcagtc
//
LOCUS       DASTMK010000476         5237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_151287, whole genome shotgun sequence.
ACCESSION   DASTMK010000476 DASTMK010000000
VERSION     DASTMK010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151287"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3267
                     /locus_tag="VFQ79_20065"
     CDS             <1..3267
                     /locus_tag="VFQ79_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142029.1"
                     /translation="LLTPSMRAQVLYGSVVGTVQDQSGAVVPGASVTLTSAGTGQTRE
                     ITTDNAGRFSVPNLVPGSYELRIAAQGFRPVVQTSVAATPNNVTRVDVKLELGATTEQ
                     VTVSAAAALLQTDKADVHMEIGAKEVTSLPLGGYRNYQSLIDLVPGATPSTTQNSIQG
                     APARALTTNINGTNRNNNITRLDGAVNLYIWLPHHTAYVAPSETVETVSIATNNFDAE
                     QGMAGGAAVTVVTKSGTNELHGSVFAYHTNNHLAAKNFFFAGDRLPKNTRNMDGFTLG
                     GPIVKNKLFFFGGWEGMRERVSSSNLYTLATAAQRAGDFSAFPTTIYDPATGTPDGRG
                     RTPFPNNIVPLNRQSAITRKMQALAPEPNLSGTASNYFKDGTQVFDRDNFDVKVNWNR
                     SDSHALFAKYSAMNANPQCNFALDEAGGPAACSGSAPGKAHTLTQISTIGHTWTFSPR
                     FVMDGTLGWTRMKQDATNPDYGTNFGLDVLGIPGTNGPDIRQSGMPKFSISGYTAMGN
                     GDNPRPNFYADQSYTSSHNASLTMGAHELRFGFDVVRHQLNHWQPELNNPRGVFTFGG
                     AETALNGGAAPNQFNSYAAFLLGLPNGMGKSLQYLTMTGREWQFGWYVRDRWQVTPHL
                     TLSLGLRYEYYPLMTRADRGLERYDLETNQVIIGRRGGNPDNVGITVSKKLFAPRVGF
                     AYRLGTSTVIRSGYGITYDPLPFSRPLRGPYPATIDQRFNGDNSFLPFRPIELGIPEF
                     SGPDINSGFIELPSTVANRSPWGGRLNRGYIQSWNFIVERKLPSQFITTFGYVGTQTV
                     HALADRNINAAAPGRGNSGRPLNATLGRAVDTNMWDGFLNANYHALQVSFNRQFSGGF
                     QMKGAYTFSKAINATDEDGWASIGWNYPDVMHRNRALAGYNRTHILQAAFIFEPPFGP
                     GKALLQDGFVSQILRNWQLSSIFSSYSGTPFTVTASGASLNAPGNSQTADQVKPEVER
                     LGGVGRNVPFYDPLAFRSVTTVRFGSTGRNILRGPGVVNTDISLSRFFPLTERFRLQL
                     RAEGFNITNTPHFNNPGANVSNMSVTPDGTITNLGNFLSITGARADERVFRFALRLEF
                     "
     gene            3271..4491
                     /locus_tag="VFQ79_20070"
     CDS             3271..4491
                     /locus_tag="VFQ79_20070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142030.1"
                     /translation="MKKLTRRQLAVGAVSLAGTRALAQQRPAPDRLRGGVIGCGIRGV
                     RAHIATLLKMKEADAVDITAVCDVFDKRAQQAAQMTGAKPYQDYRRLLEDKDIDFVVI
                     ATPDHLHAQMAIDAASAGKHIYCEKPMTHSVDEAKRVVAKVRETSVKMQVGVQGMSDD
                     SYETAYKYVKDGVLGKVVIAQIDYSRNYRGDYWLKPFDSDANPGLNLNWDLFQGPAKH
                     RPWDPERYFNWIRYYDYSGGVPAGLFVHRVTRIIKSLGLTFPEFGSAHGGTFQFTESK
                     AEVPDTINIMLDYPGGPTVLLISSLANDTPVDHVLRGHKATLEFTPTGFTIRPQKLYE
                     KEVKEIVHVKTGGEDTELHLRNLLRAIRFNEPLKCDEMLGYYGVVACQFGVLSLQRRK
                     YLKWDKAGERIVNI"
     gene            4488..>5237
                     /locus_tag="VFQ79_20075"
     CDS             4488..>5237
                     /locus_tag="VFQ79_20075"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HEU0142031.1"
                     /translation="MSRLLVCMFALWAAASCSAESGFQFRQSGTSGLELTENGKPVFV
                     YIHQPVLKTGTDEKYRRSSYIHPLYAPGGTIVTDDFPKDHPHHRGLCWAWPIVRFEGQ
                     THDMWAVQGMHQRFVRWLKRDAGTRSARIAVENGWFAGDRKAVKETVEIEAMPAAGNR
                     RELRLTLMFEAAGEPVEIEGREVKGYGGLHIRFAPRENTVIHTDKGRLERDSDLTRHP
                     WAELEGTMEGKRAGVRIDVEPSNPGTPNGWCL"
BASE COUNT         1130 a         1533 c         1570 g         1004 t
ORIGIN      
        1 ctgctcacgc catctatgcg cgcgcaggtg ctctatgggt cggtagtagg aacggtacag
       61 gaccagagtg gtgctgtggt gccgggcgcg agcgtcacgc tgacaagcgc cggtacaggt
      121 cagactcggg aaatcaccac cgacaacgcc gggcgcttct cggttccgaa cctcgtgcct
      181 ggaagctatg agctgaggat tgccgcacag ggttttcgtc cagtcgttca aaccagtgtt
      241 gccgcaaccc caaacaatgt cacgcgcgtc gacgtgaagc tggagctggg cgccacgaca
      301 gaacaggtta cagtcagcgc cgcggctgcc ctgctgcaaa cggacaaggc ggatgttcac
      361 atggaaatcg gcgccaagga agtgacgagc cttccattgg gcggctaccg gaactaccag
      421 agtctgattg atctggtgcc cggcgctacg ccatccacga cacagaacag cattcaaggc
      481 gcgccggcgc gggccttgac aacgaacatc aacggcacca accggaacaa caacattacg
      541 cggcttgacg gagcggtgaa tctttatatc tggctgcctc accataccgc gtatgtggcg
      601 ccgtccgaga cggtcgaaac cgtcagcatc gcaaccaata acttcgatgc ggaacaggga
      661 atggccggcg gggcagccgt cacggttgtg acgaaatccg gcacgaacga actacacggc
      721 tcggtttttg cctatcacac caacaatcat cttgcggcga agaacttttt cttcgccggg
      781 gaccggcttc caaagaacac ccgcaacatg gatggcttca ccctcggcgg acccattgtg
      841 aaaaacaagc tgttcttctt cggaggatgg gaaggtatgc gcgagcgtgt gagttcctcg
      901 aacttgtata cgctagcaac agcggcgcaa cgtgcgggcg acttcagtgc attccccacg
      961 acgatctacg atccggctac gggaactccc gacgggcgcg gacgcactcc gttcccgaat
     1021 aacatcgttc cactgaatcg ccagagtgcg attacgagga agatgcaagc gctggctcct
     1081 gagccgaatc tgagcggaac ggcatcgaac tacttcaagg acggcacgca ggtattcgat
     1141 cgcgacaact tcgacgtgaa ggtgaactgg aatcgcagcg acagccacgc cctgtttgcg
     1201 aaatatagcg cgatgaatgc caatccgcag tgcaatttcg cgctcgatga agcgggcggc
     1261 cccgcggcgt gctccggaag cgcgccgggc aaggcacata cactgactca gatttccacg
     1321 atcggtcaca cctggacgtt ttcaccgcgg ttcgtaatgg acggcacgct cggctggacc
     1381 aggatgaagc aggatgcgac gaacccggac tacggcacga acttcggact cgacgtgtta
     1441 ggaattccag gcaccaacgg accggatatc cggcagagcg gaatgcccaa attcagcatc
     1501 tccgggtata cggcgatggg taacggcgac aatccgcggc caaacttcta tgccgaccag
     1561 agctacacgt cgtcgcacaa cgcgagcttg acgatgggag cgcacgaact gcgcttcggc
     1621 tttgatgtcg tcaggcacca attgaatcac tggcagccgg aactgaataa tccgcgcggt
     1681 gtattcacgt tcggcggcgc cgaaaccgcg ctgaatgggg gtgccgcgcc gaaccagttc
     1741 aactcctacg ccgctttcct gctgggattg cccaatggga tgggaaagag cttgcagtat
     1801 ctcaccatga ccgggcgcga atggcagttc ggttggtacg tgcgcgaccg gtggcaggtt
     1861 actccgcacc tcaccctttc gctcggattg cgatatgaat actacccgct gatgacgcgc
     1921 gcggaccgcg gtctggagcg gtacgacctg gagacaaacc aggtgattat cggccgtcgc
     1981 ggcggcaatc cggataatgt cggcatcacc gtgagcaaga agttgtttgc tccgcgtgta
     2041 ggatttgcgt accggctcgg gacctccact gtgatccgca gcggctatgg aattacatac
     2101 gatccgctgc cattctcacg gcctctgcgc ggcccctatc cggctaccat cgaccagcgc
     2161 ttcaacggcg acaactcctt cctgccattc cggccgattg aacttggcat tccggagttc
     2221 tccgggccgg atatcaacag cggtttcatc gagcttccat ctactgtggc caatcggagc
     2281 ccctggggag gacggctgaa tcgcggctac attcaatcct ggaacttcat tgttgaaagg
     2341 aaactgccgt cccagttcat caccacattc ggctacgtcg gcacacaaac ggtgcatgcg
     2401 ctggcggacc gcaatatcaa cgccgctgcg ccggggcgag gcaacagcgg ccgtcctctg
     2461 aacgccacgc tcggacgcgc cgtcgatacc aacatgtggg acggtttttt gaacgccaac
     2521 tatcatgcgc tgcaagtgag tttcaaccgg cagttcagcg gaggctttca gatgaaaggg
     2581 gcgtacactt tctcgaaggc gatcaatgcg actgacgaag acggatgggc gtcgatagga
     2641 tggaattacc ccgacgtaat gcacaggaat cgcgcgctcg cgggttacaa ccgtacccac
     2701 attctgcaag ccgctttcat tttcgagccg ccattcggtc ccggaaaagc acttctgcag
     2761 gacgggtttg tttcccagat tctgcgcaac tggcagctta gcagcatctt cagctcctat
     2821 agcgggactc cgttcaccgt gaccgcttcg ggcgcttctc tgaacgctcc cggcaattca
     2881 caaacggcgg accaggtaaa gccggaggtg gaaaggctcg gcggcgtggg acgcaatgtc
     2941 cccttctacg atccgctggc cttccggtcg gttaccaccg tccgtttcgg ctccaccgga
     3001 cggaacatcc ttcgtggtcc cggcgtggtc aacaccgata tcagcctttc ccggttcttt
     3061 ccgctgacgg agcggttccg attgcagttg cgtgcggagg gcttcaacat cacgaatact
     3121 ccgcatttta acaaccccgg cgccaacgta tcgaatatga gcgtcactcc cgacggcacc
     3181 attacgaacc tcggcaactt cctgtcgatc accggagcgc gggcggatga gcgcgtcttc
     3241 cggttcgccc ttcggctaga attttaggcg atgaagaaat tgactcgccg tcaactcgcg
     3301 gtgggcgctg tgtccctggc agggacccgc gccctcgcgc aacagcgccc cgcgccggac
     3361 cggctgcggg gcggtgtcat tgggtgcggt attcgcggcg tacgcgccca catagccaca
     3421 ttgctcaaaa tgaaggaagc ggatgccgtg gatattacgg ccgtctgcga tgtcttcgac
     3481 aagcgcgcgc agcaggcggc gcaaatgacc ggggccaagc cgtaccagga ttaccggcgt
     3541 ctgctcgaag acaaagatat cgacttcgtc gtcatcgcca caccggacca cttgcacgcc
     3601 cagatggcga tcgacgccgc cagcgcgggc aaacacatct attgcgaaaa accgatgacg
     3661 cattcggtgg acgaagccaa gcgcgtggtg gccaaggtcc gcgaaaccag cgtcaagatg
     3721 caagtgggcg tgcagggaat gtcggacgac tcctacgaga ccgcttacaa atatgtgaaa
     3781 gatggcgtgc tcgggaaggt ggtcatcgcc cagattgact actcgcggaa ttaccgcggc
     3841 gattactggc tgaagccttt cgattccgat gcaaaccccg ggttgaatct caattgggat
     3901 ctgtttcagg gtccggcgaa acaccggccc tgggatccgg agcggtattt caactggatc
     3961 cgctactacg actattcggg cggggttccg gctggtttgt ttgtccacag ggtgacgcgc
     4021 attatcaagt ccctcggctt gaccttcccg gagtttgggt cggctcacgg cggaactttt
     4081 caattcaccg aaagcaaagc cgaagttccc gacaccatca acatcatgct cgattacccg
     4141 ggcggtccca cggtgttgct catttcgtcg ctggcgaacg acacgcccgt ggatcatgtc
     4201 ttgcgtgggc acaaagctac gctcgaattc actcccaccg gatttacgat tcgcccgcag
     4261 aagctgtatg agaaagaggt gaaggagatt gtgcacgtaa agaccggcgg tgaagatacc
     4321 gagctgcacc tgcgcaacct gcttcgggcg atccgcttca atgagcctct gaaatgcgac
     4381 gagatgctcg gctactacgg ggtagtggcg tgccagttcg gggtgctgtc gctccagcgg
     4441 cgcaagtacc tgaagtggga caaagccggc gaaaggatcg ttaacatatg agtcggctgt
     4501 tggtgtgcat gtttgcgctg tgggccgcgg cgagttgcag cgcggagagc ggctttcaat
     4561 tccgccagag cggtacgtcg ggcctggagc tgacggaaaa cggtaaaccg gttttcgttt
     4621 acatacatca gcccgtattg aagacgggca cggatgagaa atatcgccgc tccagttaca
     4681 ttcatcccct ctatgcgccc ggcggcacca tcgttaccga cgatttcccg aaagatcacc
     4741 cgcatcaccg ggggctctgc tgggcgtggc ccattgtacg tttcgaaggg cagactcacg
     4801 atatgtgggc ggttcagggc atgcaccagc gcttcgtccg gtggttgaag cgggacgcgg
     4861 gcacgcgcag tgcacgcatt gccgttgaaa acggatggtt cgcgggagac cggaaagccg
     4921 tcaaagagac cgtcgagata gaagccatgc cggcagcggg caaccgccgc gagttaaggc
     4981 tgacccttat gtttgaagcg gccggcgagc ctgtcgaaat cgaagggcgc gaagtgaaag
     5041 ggtatggcgg attgcacatc cggttcgcgc ctcgcgaaaa taccgtgatc cacacggaca
     5101 agggccgtct tgagcgggac agcgacttga cccggcaccc gtgggcggag ctcgaaggga
     5161 cgatggaagg caagcgggct ggtgtgcgta tcgatgtgga gccgtcgaac cccggcacgc
     5221 cgaatggctg gtgcctg
//
LOCUS       DASTMK010000477         6935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_440373, whole genome shotgun sequence.
ACCESSION   DASTMK010000477 DASTMK010000000
VERSION     DASTMK010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440373"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFQ79_20080"
     CDS             <1..498
                     /locus_tag="VFQ79_20080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142032.1"
                     /translation="QLASLNMAFVYSARGDRNAALNQIQKALEIDPTCAKSYLNLALA
                     YRAQSRPEDAAAAFERARELGAAEPRALARLGAIYATAGKMDEALRTLSDVERLAGSR
                     YVSSFDLALLHAAVGHTERAVQLLERAYQERSAGMIYLRMEKIGELKSHPRVRELVSR
                     MRLPG"
     gene            complement(525..926)
                     /locus_tag="VFQ79_20085"
     CDS             complement(525..926)
                     /locus_tag="VFQ79_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142033.1"
                     /translation="METHVRILGYLYLVFGCLGVLGGLVALVFFGGIAGLAGVAGSGP
                     DAWVAVPILGGIGGILFVIVLLFSLPAVIAGAGLLQFKNWARILAIILSAINLLNMPF
                     GTALGVYGLWALLSNDTVRLFLPRPSPPSRL"
     gene            complement(955..2016)
                     /locus_tag="VFQ79_20090"
     CDS             complement(955..2016)
                     /locus_tag="VFQ79_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0142034.1"
                     /translation="MSKMLEEIRQQPSALERTLKTELPRAERFRKRLIEKPPRFIVLA
                     ARGTSDNAALFGRYLVEITTGIPVSLAAPSILTLYNASVSLEDALVVAISQSGESTDT
                     NFVLERAKASGAVTVGITNEANSTTANMADHVFLVRAGKEKSVAATKTYTGQLLSMYL
                     LAYALGAPIRPDSLKRIPEWAHAALKLEPEIASRAGRYRFMDHAVVVGRGLNYSNAFE
                     FALKMMETCYVVAERFSSADFLHGPIAMVEPSFPMFLFAPPGVTWPSIQEMLQKLESL
                     KAETLVITDTGNRSALSVAKRAVAVPVKLGQKGEAVEELYTPIPYIIPAQLFAAYLAA
                     EKGLDPDKPRTLQKVTRTL"
     gene            complement(2050..3606)
                     /locus_tag="VFQ79_20095"
     CDS             complement(2050..3606)
                     /locus_tag="VFQ79_20095"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEU0142035.1"
                     /translation="MAQSARDLYRQGRKAEERGELARAYLLYAAAAAVDPGNRGYWAR
                     SQALRTKAVLESSPVAASMDTSLTDLPDPEQAAAESLLGKLTDLDLKEARELLPPPEL
                     RPALTRTDFDLKLDSKQLFEKVAAAYGLDVVFDGDFKPSPPIRFAIQNAGFREALRAL
                     EAASASFIVPISERLFLAVNDSPQKRAAVEPTVAAVIPIPEPVSIQEAQELARAVQQT
                     MEIPKFVIDSQRRIVVMRDRVSKIQPAQALFHQMLAAPGQVSVEVEFLEVNKSSSLSY
                     GLNLQRAFPIVAFGKLWNSKPSIPSGFTRFLTFGGGKTLFGIGITDPEIFASMSKFNS
                     QTLLLSEIRSVDGQPATLHIGDKYPIITGGYYGDTSGTGQVYTPPPTFNFEDLGVEIK
                     ITPHVHGAAEVSLELEAEYKVLTGETLNAIPVISTRRFVSTVRLKTGEWAVVGGLLNS
                     SEAKTITGLAGLSSIPILGPLMRQNTRDKTSNEVLLVLKPKLLTLPPREVIATRSFWV
                     GTDTRPIAPI"
     gene            3726..4994
                     /locus_tag="VFQ79_20100"
     CDS             3726..4994
                     /locus_tag="VFQ79_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0142036.1"
                     /translation="MAATVPALGTQFRPKAPQTFEEAGVSQTLVTDLVLKRTLLQGFA
                     TLGALSGALKLSPAIIEVVFRQLRQQQLIEIKGMIGNDYQFVLSGAGRTLAADRFQIN
                     QYASACPVPLAGYHAGAKAQAAKVESDRRSIRRAFNDLVLTDRLLDQLGPALISQRAI
                     FLYGPSGNGKTSIAERMLRVYQDAVLIPHAIEVDGQIIGVYDPVVHESLEFMDPDIDP
                     RWVVCRRPCISVGGELVPSMLELRLDDVSGIYAAPLQMKANNGLLIIDDFGRQLMSPR
                     DLLNRWIVPLDRRVDYLTLRYGVKFQIPFEVQVVFSTNLDPHDLADEAFLRRIQNKIY
                     VEPVTGEVFDEIFRRVTTGRSVPCEEDSAAFLRELSLREGRRELRACFPLDICNILEA
                     VAKYEKRTVQINREELERAAGMYFAKTEDV"
     gene            5036..6514
                     /gene="gatB"
                     /locus_tag="VFQ79_20105"
     CDS             5036..6514
                     /gene="gatB"
                     /locus_tag="VFQ79_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU0142037.1"
                     /translation="MSSSTLVLPPPEVVARYEPVIGLEVHVQLATQTKIFCGCPTSFG
                     APPNTNVCPVCLGLPGALPVLSRQSVEMAIRAALALNCQVRPVSRFARKNYFYPDLPK
                     GYQISQYDQPVAENGSVEIVVDGNAKRIGITRVHMEDDAGKSIHEGFRDSDRYTYVDL
                     NRCGTPLIEIVSEPDMRSSEEAYAFLTELKHLLQWVEVSTCDMEKGHLRCDANVSVRL
                     HGAPQFGTKAEVKNLNSFRFLKMALDYEIARQIALIEGGGSVEQETRLFNPDTGETAG
                     MRSKEHAHDYRYFPEPDLVPLKISQDWLSRVESEMPELPNRRRERFIAGYGIREYDVQ
                     VLTATRALSDYYEETASKSGDPKAAGNWVMGDLMGALKSEGKEITESPVSAANLAELV
                     DLIASGEISGKLAKEIFQKMFATGESARVIIDREGLRQISDAGVLERMVDEVIANHPK
                     QVDQYKGGKTTVLGFLVGQVMKASRGQANPGAVNELLRKKLS"
     gene            complement(6528..>6935)
                     /locus_tag="VFQ79_20110"
     CDS             complement(6528..>6935)
                     /locus_tag="VFQ79_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142038.1"
                     /translation="ELTARPDNRSGRAVFLVRDSEGGDDKYTIEFQWSGSGGLGGGRD
                     SAGGFGRPGSRPGSTFNNDDALRVCRDAVTTRIQAGYQGADVDFASPAAGQRDVIQGE
                     AIAWRGRDRQWFRYTCRVDFNSRRVRDVNVTRR"
BASE COUNT         1482 a         2058 c         2036 g         1359 t
ORIGIN      
        1 caactggcaa gcttgaacat ggctttcgtt tactcggcgc ggggcgaccg gaatgccgcg
       61 ctcaaccaga ttcaaaaggc tctggaaatc gatcccactt gcgccaagag ctacctcaac
      121 ctcgccctcg cttaccgcgc ccagtcacgg ccggaagatg cagcggccgc ttttgagcgt
      181 gcccgcgagc tgggcgccgc ggaacctcgc gcgctggcca ggcttggagc tatttacgca
      241 acagcgggaa aaatggacga ggcgctacgc accctctccg acgtagagcg gctcgccggc
      301 agccgttatg tgtcttcttt cgatcttgcg ctgttgcacg ccgctgtggg ccacacggag
      361 cgtgccgttc aattactcga gcgcgcctac caggagcgct ccgccggcat gatctatctg
      421 aggatggaaa agatcggcga gctaaagtcg caccctcgtg tccgggagct tgtaagccgc
      481 atgaggcttc ccggatgagg cggtgctttc gaaggctgtg aatttcagag ccgcgatggt
      541 ggtgatggac gcggcagaaa gaggcgcacc gtatcgttcg acagcaatgc ccacaggccg
      601 tacacaccca gtgcggtacc aaacggcatg ttcaacaggt tgatcgccga caggatgata
      661 gccagaatac gcgcccaatt cttgaactga agcagaccgg ctccggcgat caccgccggc
      721 aggctgaaca gcaacacgat cacgaaaagg attccgccaa ttccacccag aatcggaacc
      781 gccacccagg catcggggcc tgatcccgca acgccggcca gccccgctat gccgccaaaa
      841 aacaccaatg caaccaggcc gcccagcacg cctaagcacc caaaaaccag gtacagataa
      901 ccgagtattc tgacatgcgt ctccatgccg ccgcctcccg cggatggaac tatgctacag
      961 cgtccgggtc actttttgaa gcgtgcgcgg tttatcggga tcgaggccct tctccgccgc
     1021 caggtacgcc gcgaacaact gcgccgggat gatatacgga attggagtat agagttcctc
     1081 caccgcttcc cccttctgcc ccagctttac cggcacggcg accgcgcgct ttgcaacgct
     1141 caaggcggaa cggttgccgg tatccgtgat caccagggtc tcggctttca gactttccag
     1201 cttctgtaac atctcctgta tcgaaggcca agtgacgccc ggcggggcga aaaggaacat
     1261 cgggaacgac ggctccacca tcgcgatggg tccgtgtaga aaatcggccg aggagaaccg
     1321 ttccgccact acgtagcagg tttccatcat cttgagcgca aactcgaagg cgttcgaata
     1381 attgagacca cggcccacga cgaccgcatg atccatgaac cggtaacgtc cggcgcgcga
     1441 agcgatctcc ggttccagct tcagggccgc gtgcgcccat tcgggaatgc gtttcagcga
     1501 gtccggccgg atgggagctc ccagggcata ggccagcaga tacatcgaca ataattggcc
     1561 ggtataagtc ttggtcgcgg caacactctt ctctttgccg gcacgaacca gaaaaacgtg
     1621 atccgccatg ttggccgtgg tgctgttcgc ctcattggtt atgccgacag tcaccgctcc
     1681 cgaagccttc gcccgttcca gtacgaaatt cgtgtcggtg gattcaccgg attgcgagat
     1741 cgcgaccacc agtgcgtctt cgagtgaaac ggaagcgttg tacaaagtca gaatggaggg
     1801 cgccgccagc gaaacgggaa tacccgtcgt gatttcgacc aggtagcggc caaataacgc
     1861 cgcgttatcg gacgtgcctc gcgcagccag tacgatgaat cgtgggggtt tttcgataag
     1921 cctctttcga aaccgctccg cgcgcggcaa ctccgtctta agcgtccgtt ccagcgcaga
     1981 aggctgctgg cggatctcct ccaacatttt cgacatcgat ttgagaatat cagaaccgat
     2041 gcaccggctc taaattggag cgatgggccg ggtgtcggtg cctacccaga atgacctggt
     2101 ggcaatcact tcgcgcggtg gcagtgtgag cagcttcggt ttcagaacca gcagcacttc
     2161 gttagaagtc ttgtcacggg tgttctggcg cattagcggg cccagaatcg gaatactgga
     2221 caatccagcc agacccgtga tggttttcgc ttcggaggag ttcaacagac cgcctaccac
     2281 ggcccattcg ccggttttta accgtacagt tgaaacgaac cggcgtgtgg aaatcaccgg
     2341 aatagcgttc agcgtttcac cggtcagaac cttgtattcc gcttccagtt cgagcgaaac
     2401 ttcggccgct ccgtgtacat gaggagtgat tttgatctcc actcccaggt cttcgaagtt
     2461 gaacgtcgga ggaggggtat aaacttgtcc cgtgccgctg gtatcgccgt aataccctcc
     2521 ggtgatgatc ggatatttgt cgccgatgtg cagcgtagcc ggctgtccat ccacgctgcg
     2581 aatctctgag agcagcagcg tctgagagtt gaacttgctc atactggcaa agatctccgg
     2641 atcggtgatc ccgatcccga acagtgtctt tcctccgccg aacgtgagga accgcgtgaa
     2701 tccgctcgga atggagggtt tcgaattcca cagcttgcca aaggcgacaa tggggaacgc
     2761 ccgctgaaga ttcaggccgt agctaagaga cgaagacttg ttgacttcga gaaattccac
     2821 ctccacggag acctgtcccg gcgctgccag catctggtgg aaaagcgcct gtgccggctg
     2881 gatcttcgaa acgcgatcgc gcatcaccac aatacgccgc tgcgagtcga tcacgaactt
     2941 gggaatttcc atggtctgct gcaccgcgcg agccaactcc tgcgcctcct gtatgctgac
     3001 cggttccgga atcggaatga cagcggcaac agtcggctcg acggcggccc gcttctgcgg
     3061 actatcgttc acagcaagaa aaagccgttc gctgatggga acgataaagg atgcgctcgc
     3121 ggcttcgagc gcccgcagcg cctcccggaa accggcattc tgaatcgcga accggatggg
     3181 tggtgacggt ttgaagtcgc cgtcgaaaac gacgtcaagg ccgtaggccg ccgctacctt
     3241 ttcgaagagc tgtttggaat cgagcttcaa atcgaagtcg gtacgcgtca acgcgggacg
     3301 caattccggg ggcggtaaca actcccgggc ttctttcaga tcgagatcgg tcagctttcc
     3361 aagcagcgac tccgcggcag cctgctccgg atccggaaga tctgtgagac tggtgtccat
     3421 gctcgccgct accggtgaag actcgagcac ggccttagtc cgcagagcct ggctgcgagc
     3481 ccaatacccc cggtttccgg ggtccacggc ggcggcggcg gcgtagagca ggtacgcgcg
     3541 cgccagttca cctcgctctt ctgcctttcg tccctggcga taaagatcgc gggcggactg
     3601 cgccattact gttccggcca ggagcaggca aatgagtaga cggaatgacc ccaaacccat
     3661 atgacgaagg aatgcggtcc ggcgtgcata aactccttgg ccgcgcccgc cgattaagaa
     3721 cctgtatggc ggcaaccgtc ccggcactcg gcacgcagtt ccggccgaaa gcacctcaaa
     3781 cctttgaaga agctggagtt tctcagacgc ttgtaacaga cctggtactg aagcgcaccc
     3841 tgctgcaggg ctttgccaca ctaggcgcgc tgagcggtgc cttgaagctt tcaccggcta
     3901 ttatcgaagt ggtcttccgg cagttgcggc agcaacagct tatcgaaatc aagggaatga
     3961 tcggcaacga ctaccagttt gtactgtccg gcgccgggcg tacactggct gcggatcgct
     4021 ttcagatcaa tcagtacgcc agcgcgtgcc cggttcccct ggccggctat cacgcgggag
     4081 ccaaggcaca ggcggcgaaa gtggaatcgg accgtagaag catacgccgg gcgttcaacg
     4141 acctggtgct gacggaccgg ctgctggatc aactgggtcc ggcgctgatt tcccagcgcg
     4201 cgatcttcct ctacggaccg agcggcaatg gaaaaaccag catagccgaa cgcatgctgc
     4261 gcgtgtacca ggatgcggtg ctgatccctc atgcgatcga agtggacggg caaattatcg
     4321 gcgtctatga cccggtggtt catgaaagtc tggagttcat ggacccggac atcgatccca
     4381 gatgggtggt ctgccgccgc ccgtgtattt ccgtgggtgg agaactggtc cccagtatgt
     4441 tggaacttcg cctggatgat gtttccggta tctatgccgc gccgctccag atgaaggcga
     4501 acaacgggct gttgatcatc gacgactttg gccgccaact gatgtctcct cgcgacctgc
     4561 tgaacaggtg gatcgttcca ttggaccgcc gtgttgatta cctcacactg cggtatggtg
     4621 tgaaatttca gattcctttc gaggtgcagg tggtgttctc gacaaacctg gacccgcacg
     4681 atctggcgga cgaggccttt ctccggcgca tccagaacaa gatttacgtc gagccggtca
     4741 cgggggaggt cttcgacgag atcttccggc gcgtaaccac cggcagaagc gttccctgcg
     4801 aagaggacag cgcggcgttt ctgcgcgagc tttctcttcg ggaaggcaga agggagctgc
     4861 gagcctgctt ccctctggat atctgtaaca tcctcgaagc cgtcgcaaaa tacgaaaaac
     4921 ggacggttca gattaatcgc gaagaactcg aacgcgcagc cgggatgtat ttcgccaaga
     4981 ccgaagacgt ttaggaggga aaggcccggg ctgggccggc cactataatg taggaatgtc
     5041 ttccagtacg ctggtgcttc cgccgcccga agtcgtagct cgatatgagc cggtcattgg
     5101 attggaagtc catgttcaac tggcgacaca gacgaaaatc ttttgcggtt gccccaccag
     5161 cttcggtgct ccgccgaaca cgaatgtttg cccggtatgc ctggggttgc cgggagcctt
     5221 gccggtcctg agccgccagt ctgttgaaat ggcgatccgc gcggcactcg ccctgaattg
     5281 ccaggtacgg ccggtctcgc gttttgcccg gaaaaactac ttttatccag acctgcccaa
     5341 gggctatcag atctcgcaat atgatcagcc ggtagccgaa aacggctctg tcgaaattgt
     5401 tgtggatggc aacgcgaagc ggattggcat cacccgcgta cacatggaag atgatgcggg
     5461 caagagtatc cacgaaggtt tccgcgattc cgaccgctac acttacgtcg atttgaaccg
     5521 ttgcggcaca cccctgatcg aaatcgtcag cgagcccgac atgcgcagct ccgaggaagc
     5581 ctatgccttc ctgaccgagc tcaaacacct tttgcagtgg gtggaggtct cgacctgcga
     5641 catggaaaag ggtcatcttc gctgtgacgc gaacgtttcc gtacgccttc acggcgcgcc
     5701 gcagttcggc accaaagccg aagtgaagaa tttgaactct ttccggttcc tgaagatggc
     5761 tctcgactac gaaatcgccc ggcagatcgc gttgatcgag ggcggtggca gcgtggagca
     5821 ggagacgcgc ctctttaatc ccgacacggg tgagacggcg ggcatgcgca gcaaggaaca
     5881 tgcgcacgat taccgctact tcccggagcc ggacttggtg ccgctgaaga tcagccagga
     5941 ttggctcagc cgtgtcgagt cggaaatgcc cgagttgccc aatcgccggc gggaacggtt
     6001 catcgcggga tatggcatcc gcgaatatga cgtccaggtg ctcaccgcca cccgcgcact
     6061 gagcgactat tacgaagaaa cggcctcgaa atccggcgat ccgaaagcag cgggcaactg
     6121 ggtaatgggc gatttaatgg gcgccctaaa gtcagagggc aaggaaatca cggagtcgcc
     6181 cgtaagcgcg gcgaacctcg ccgaactggt ggacctgatc gccagcggtg aaatctccgg
     6241 caaactggcc aaggagatct tccagaagat gttcgctacg ggcgaatctg cgagggtcat
     6301 catagatcgc gagggcttgc ggcagatcag cgacgccgga gtgctcgagc ggatggtgga
     6361 cgaggtgatt gccaaccacc ccaagcaggt ggatcagtac aaaggcggca agacgaccgt
     6421 tctcggattc ctggtcggcc aggtgatgaa agcctccagg ggacaggcca atccgggggc
     6481 cgtgaacgaa ttgctcagga aaaaactttc gtaacgggct cgcctgctta ccgcctggtg
     6541 acgttcacgt cccgcacgcg gcgggaattg aaatcgacac ggcatgtata gcggaaccat
     6601 tgccggtccc gcccccgcca cgcaatcgct tctccctgta tgacatcgcg ctggcccgcg
     6661 gccggtgacg cgaaatccac gtctgcgccc tgataccccg cctgaatgcg tgttgttacc
     6721 gcatccctgc aaacccgaag tgcgtcatca ttgttgaacg tgctgcccgg ccgcgatcct
     6781 ggacggccga atccgcctgc gctgtcccgg ccgccgccca gtccgcctga gcccgaccat
     6841 tgaaactcga ttgtgtattt atcgtcgccg ccttcggagt ccctgaccag aaacacggca
     6901 cgtccggagc gattatcggg gcgggccgtc aactc
//
LOCUS       DASTMK010000478         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_376274, whole genome shotgun sequence.
ACCESSION   DASTMK010000478 DASTMK010000000
VERSION     DASTMK010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376274"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..360
                     /locus_tag="VFQ79_20115"
     CDS             100..360
                     /locus_tag="VFQ79_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU0142039.1"
                     /translation="MAYVIAEPCIGTKDTACVDACPVDCIHPKKDEGEYETAELLYID
                     PVECIDCGACVPVCPVSAIFALDDLPEKWANFAQINADYYKR"
     gene            complement(374..754)
                     /gene="queD"
                     /locus_tag="VFQ79_20120"
     CDS             complement(374..754)
                     /gene="queD"
                     /locus_tag="VFQ79_20120"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEU0142040.1"
                     /translation="MFEVSVEETFAAAHQLRNYHGKCENLHGHNYRVRVTIRGRELDE
                     TGLVVDFVELKRVMRAVIDKLDHTYLNEVPPFDILNPSAENVAKYFHDEVSSGLRAGD
                     RKTAVEVAEIKIWETDTSTATYRP"
     gene            complement(747..2675)
                     /locus_tag="VFQ79_20125"
     CDS             complement(747..2675)
                     /locus_tag="VFQ79_20125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEU0142041.1"
                     /translation="MAAETKPLSVRRFFARTGLLSQWHPNFEYRPGQLEMAEAVENAL
                     TSRKHLIVEAGTGTGKTLAYLMPAILSGKRVVVSTGTKNLQEQLFFKDVPFLERHFER
                     PLRVCYMKGRSNYACRQKIYDAEREPILSGLEEVADFQIIRDWEQHTETGDRAEIKTL
                     PENSSAWSKIDARRDLCTGQKCQQFERCFVTLMHQRAIESDIIIVNHHLFFADLAVKD
                     EDYSGIIPEYHAVIFDEAHEIEDVAGQYFGVSVSNFQFEELRRDVLAVARRKDFGSAE
                     LDRILITAEQCANRFFSLFGNVEGRTGFRGQQAFLENHEEEYRDVLRSLELISMQLQL
                     IKQQPEEIIPLHRRSTELGDRLEFWMAGCDSRYVYWIERRGRGCFLQATPIDVSEILE
                     QRLFEEVDAVVLTSATLAVGGGFDYVSRRLGARNAGTLIVPGHFDYERQALFYVPQHM
                     PDPRSPAFAAVAADEIKQLLKLSRGRAFVLFTSYQQMRIVYDRVSLAIEYPTLMQGTG
                     PRSALLEEFRQTPNCVLFATSSFWQGVDVPGDQLSCVIIDKLPFAVPSDPVVEARVAS
                     IRADGGNPFYDYQIPQAAIALKQGFGRLIRSRTDRGVLALLDNRITKQRYGQVFFDSL
                     PGYGFTTRIADVEKFFNV"
     gene            complement(2723..3610)
                     /locus_tag="VFQ79_20130"
     CDS             complement(2723..3610)
                     /locus_tag="VFQ79_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0142042.1"
                     /translation="MQPAIEKTGLRPLEVGLMFWGEKAPLNTIRAVKNLGLKCGHLGF
                     AGHVNLKETAQAWRDALEQEQFVVTTIWASYDGESYADGPTVQRTVGFIPPATREARE
                     ARTREVIDAGALMGIGSFGCHIGFVPEDTSNPDFLAVRDIVRRICDYAGERGMTFALE
                     TGQEPAEVLKHFIQEVGRDNLKINFDPANMILYGTADPIEALDIVAPWVVSVHAKDGN
                     WPPKDQPGTLGTEMPLGQGAVGMERFVNKLKEIGYQGPMAIEREVALDQDMDDRHKEG
                     LSHLDDIKQAIALLERLRG"
BASE COUNT          759 a         1147 c         1072 g          814 t
ORIGIN      
        1 ctgaaggcac caaaacgcct tccactttgt ccaacccgcc gggatgtgct atagatgttg
       61 tttctgtagg actcggaggg agaagaggga gtaacgttta tggcctatgt catcgcagaa
      121 ccctgcattg ggaccaagga tacggcgtgt gtggacgcat gcccggtaga ttgcatccat
      181 cccaagaaag atgaagggga gtacgagacg gccgaactgc tgtacatcga tccggtagaa
      241 tgcatcgact gcggcgcgtg cgtaccggtc tgccctgtgt cggcaatctt cgcactggac
      301 gatctgcctg agaagtgggc aaatttcgct cagatcaacg ccgattatta caagcgctga
      361 agcccgtcgc cggctagggc ctgtaagtcg ccgtgctggt atccgtctcc cagatcttga
      421 tttccgccac ttcgaccgcc gtcttgcggt ctcctgccct taatcccgaa gagacctcgt
      481 cgtggaaata tttcgccacg ttttccgccg aggggttgag gatatcaaat ggcggcactt
      541 cgttcaggta cgtgtgatcc agtttgtcga tgacagcgcg catgacgcgc ttcagctcca
      601 cgaaatccac aaccagcccg gtctcatcca actcccgccc gcgaatggtg acccgtaccc
      661 ggtaattgtg gccgtgcaaa ttctcgcact tgccgtgata attgcggagc tggtgcgccg
      721 ccgcgaacgt ttcctctact gaaacttcaa acattgaaaa acttctccac gtcggcgatg
      781 cgggtcgtga agccgtagcc gggaaggctg tcgaaaaaga cctgtccgta tcgctgtttc
      841 gtaatccggt tatccagcaa cgccagcacg ccgcgatctg tccggctgcg gatcagacgg
      901 ccgaatccct gcttcagggc gatggccgct tgcgggatct ggtaatcgta gaatggattc
      961 cccccgtccg cgcgaatgct cgccacccgg gcttcgacaa cgggatcgct cggaaccgcg
     1021 aatggcagtt tatctatgat aacgcagctc aactggtccc ccggcacgtc tacgccctgc
     1081 cagaacgacg acgtggcgaa caatacgcaa ttgggggttt gccggaactc ttcgagcagt
     1141 gcgctgcgcg gcccggtgcc ttgcatcagt gttggatatt cgatcgccag cgacacacga
     1201 tcgtacacaa tccgcatctg ctggtagctg gtaaacagga cgaacgcgcg cccgcggctg
     1261 agcttcaaca attgtttgat ctcgtccgcg gccaccgctg cgaaggccgg gctgcgtggg
     1321 tcgggcatgt gttggggaac gtaaaagagc gcctgccgct cataatcgaa atggccggga
     1381 acaatcagcg ttcccgcgtt gcgcgcgccc agccgccgcg atacatagtc gaaaccgcca
     1441 cccacggcga gtgtcgccga agtgagcact acggcatcca cttcttcaaa gagccgttgt
     1501 tccaggatct ccgaaacgtc tatgggcgtc gcttgcagga agcagccgcg gccgcggcgc
     1561 tcgatccagt agacgtatcg cgaatcgcaa cctgccatcc agaactcgag ccggtcgccc
     1621 agctccgtcg agcggcggtg aagcggaatg atctcttcgg gctgctgctt gatcaactga
     1681 agctgcatcg agatcagctc cagcgatcgc agaacgtccc ggtattcctc ctcatggttt
     1741 tcaaggaacg cctgttgccc ccgaaaaccg gtccggccct ccacgttgcc gaacagggag
     1801 aagaaacggt tggcgcattg ttccgctgtg atcaggatgc gatccaactc cgccgaaccg
     1861 aagtcttttc gccgcgcgac ggcgagaaca tcgcgccgca gttcctcgaa ctggaaattg
     1921 ctgacggaca cgccgaaata ttgtcccgcc acatcttcaa tctcatgcgc ctcgtcgaaa
     1981 ataacggcgt gatattcggg aataatgccg ctataatctt cgtctttcac cgccaggtcg
     2041 gcgaaaaaca ggtggtgatt gacaatgatg atgtcgcttt cgatagcccg ctggtgcatc
     2101 agcgtgacga agcagcgctc gaactgctgg cacttctgcc cggtgcaaag gtcccgccgc
     2161 gcgtcgattt tactccaggc ggagctgttt tcgggcaggg tcttgatctc ggcgcgatcg
     2221 ccggtttccg tgtgctgctc ccagtcgcga atgatctgaa aatcggcgac ctcttcgagg
     2281 ccgctcagga tcggttcacg ctcggcatcg tagattttct gccggcaggc gtagttgctg
     2341 cggcccttca tgtaacagac gcgcagagga cgctcgaagt gccgttcgag aaacggcacg
     2401 tctttgaaga acagttgctc ctgaaggttt ttggtgccgg tggacaccac cacgcgcttt
     2461 ccggagagaa tcgcgggcat cagataggcc agcgttttgc ccgttccggt gcccgcttcc
     2521 acaatcaggt gtttacgcga agtgagcgcg ttttccaccg cctcggccat ctccaactgg
     2581 cccggcctgt actcgaaatt cgggtgccac tgagacagca atcccgttcg cgcgaagaac
     2641 ctgcgcacgg agaggggttt ggtttcggcg gccatccgga tgatttcagt atacgtgccg
     2701 gaggccgccc gattatcgcg cttcagccgc gcagccgctc gagcaaagca atcgcctgct
     2761 tgatgtcgtc caggtgcgac aggccctctt tatgccggtc gtccatatcc tggtcgagag
     2821 cgacttcgcg ctcgatcgcc atcggtccct gatagccgat ctccttcaac ttgttgacga
     2881 agcgctccat gccgaccgcg ccctgcccga gcggcatctc cgtcccgagc gtccccggct
     2941 ggtcctttgg aggccagttg ccgtccttcg cgtgtacgga gacgacccag ggagctacga
     3001 tatcgagcgc ctcgatggga tccgccgttc cgtacaggat catgttggcg gggtcgaagt
     3061 tgatcttcag gttgtcgcgg ccaacttcct ggatgaagtg cttcagaacc tccgcgggct
     3121 cctgtcccgt ttcgagcgca aacgtcatgc cccgctcgcc ggcgtagtcg cagatgcgcc
     3181 gcacaatatc gcggaccgcg agaaaatccg ggtttgaggt atcttccgga acaaaaccga
     3241 tatggcagcc gaaactgccg atacccatta gggctcccgc atcgatcact tcgcgcgtgc
     3301 gcgcctccct tgcctcgcgt gttgccggcg gaatgaagcc cacggtgcgc tgaaccgttg
     3361 gcccgtcggc atagctctcg ccgtcatacg aagcccagat ggtggtgacg acaaattgtt
     3421 cctgctccaa cgcgtccctc cacgcctgtg cggtctcctt caaattcaca tggccggcga
     3481 aacccaggtg cccgcatttc aaaccaaggt tcttcacggc gcggatggtg ttcagaggcg
     3541 ctttctctcc ccaaaacatc aggccgactt ccaacggccg caagcccgtt ttctcaatgg
     3601 caggttgcat ctgttcttcc tttcgtttcc taaacagaca tcatacgtgt aaacatggac
     3661 gcgagtacac gagggttgcc gcaaatcagt tcaaaatcgt ggacgggcat ccagaaacgt
     3721 ttgttcctct attgacaccc cgggatgtct gttttgaaat actctagagt gatggcgaag
     3781 aagtggtatg cg
//
LOCUS       DASTMK010000479         4928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_600567, whole genome shotgun sequence.
ACCESSION   DASTMK010000479 DASTMK010000000
VERSION     DASTMK010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600567"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="VFQ79_20135"
     CDS             complement(<1..1773)
                     /locus_tag="VFQ79_20135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0142043.1"
                     /translation="MRARTISHYEIVDKLGEGGMGVVYKARDTHLDRFVALKILSDAN
                     ALDTEGRMRFAQEARSASALNHSSIITIYEVGTEDGSDFIAMEYVDGKTLDELIGSHG
                     LRYDTALKYAVQIADALAAAHSAGIVHRDIKPRNVLVTGRGMVKVLDFGLAKRLFLSA
                     DASSGAMGELPHTREGVVLGTLSYMSPEQAEGQPIDSRSDIFSFGAMLYEMVTGRKPF
                     SGSSRLATLAAIVRSEPVPVTQVREDVPRELERIIMRCLRKQPAKRFQTMADLKVALD
                     EIREESDSGKLVPPPPPKPPSRHKWVYAGLGVLAFLAGFFAWWVIRAGSEQSLPVRVV
                     PLTTYPGDESRVTFSPDGSQMAFSWNGPDRDNVDVYVRLINGGTALRLTSDPGEDLSP
                     AWSPDGRIIAFVRRSGRSAEIRLVSPLGGPERKLADLAFSPPFYSQVRLLAWSHDGES
                     LVAIDRESDKSPLSAFLIHVATGNKTRLSRPPETAFGDTVASPSPDGTRLALVRWSTS
                     SNADLYVQRFDGSDLRRVTADEKRIWGVDWTSDGRELVFSSNRSGTPLIWRVDATKSG
                     LASPRLISGTEGDAWFPVIHHSPAG"
     gene            complement(1875..4298)
                     /locus_tag="VFQ79_20140"
     CDS             complement(1875..4298)
                     /locus_tag="VFQ79_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEU0142044.1"
                     /translation="MIHSSADLLEFESLRQILSRYLASEFGRAELEKVAPGTDRLLLE
                     EMHAEVGEAIGYLEAARKPQPAARGAAVRIRFDLPDAAIAIRKVSIEGAVLETKEIAA
                     LTDVLGRAAEARGLLMAVEGRFPRLGARGAQIGEFRPVLADLSGKILPDGTVADHASV
                     ALARLRREMERQRKNIQESLERFLRAHREEGILQEEFVTIRNDRFVVPLIAGQQRKVE
                     GVIHGSSGSGHTLFVEPLETIHLNNELVRLSEEELREVHRILREMTERLRMHASAIAQ
                     TAQVFGELELLFAKAQFALDFGAVVPRFSPPSERRLVLEDARHPLLEDVLKRQHRYAV
                     PFSVKMDDNCRTLLVSGPNTGGKTVTLKTVGLLTLMAQAGLPVTCAQAEFPIFDRVLA
                     DIGDQQSIQESLSTFSAHIARIRDMVEEVTPDSLVVLDELGRATDPEEGGALGVAVLE
                     HFRKAGAFAIASTHLLALKIYGANTEGVLNGSMGFDENTLEPTYRLQLGAPGKSAGLD
                     IAARLGMPAEIIESARASMSGSDRDVARFLDEIHKRIEAVSQTEKQLQEEREALEARE
                     RSTVKEWQQKQAAKLRELERRCDALMKEFEGHAHETIDRILQTAEQRKAAAQAKRQVA
                     RTRREFEEEFRATVLSTQDEAQQAQVAPKARIEEGARVRLAGVSEIARVRRLLPHDMI
                     EVEIGFMKMQAAAGDVTEVLPETGEATWLPKNVSFQQGPTWNVLQREINVIGMRAEEA
                     IDAIDKFLDSAALAAVQRVRIVHGHGMGVLKRAISEFLTNSPYAEKFYPAPPSEGGSG
                     ATIVELKAS"
     gene            complement(4295..4762)
                     /locus_tag="VFQ79_20145"
     CDS             complement(4295..4762)
                     /locus_tag="VFQ79_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0142045.1"
                     /translation="MPRNSKVQDSRRYPKRPILGVGALVFRRNRILLVERGREPLRGY
                     WSLPGGVVEVGETLIEAVQREVLEETGLRVKALAVVEIFERIMHDAEGAPEYHYVLVD
                     YICRPAAGELQPADDVSDASWVTERELAGYKLTEGTLEVIQKGFRERSNLRFL"
BASE COUNT          910 a         1614 c         1444 g          960 t
ORIGIN      
        1 acctgccggc gaatgatgaa ttacggggaa ccacgcgtct ccctcggttc cggaaatcag
       61 ccggggcgac gccaaccccg attttgtggc gtccacgcgc cagatcagcg gcgtgccgga
      121 acggttcgag gaaaagacaa gttcgcggcc atcgcttgtc cagtccacac cccaaatccg
      181 cttttcatcg gcagttaccc gccggagatc ggatccgtcg aagcgctgga cgtaaagatc
      241 ggcattggaa ctggtagacc agcgaaccag cgccaggcgt gttccatcgg gtgaaggtga
      301 ggcgaccgta tccccgaagg ccgtttcagg aggtctggag agccgcgttt tgttcccggt
      361 agcgacatga atcagaaacg ccgacagcgg cgatttgtca ctctcgcgat cgatcgctac
      421 cagggactct ccatcatgcg accaagccag caggcggacc tgggagtaga acggcggaga
      481 gaaggcgagg tcggccagtt tgcgctccgg cccgcccagc ggagaaacca ggcggatctc
      541 cgcggaccgg cccgatcgcc gcacgaatgc gataatacgc ccgtcgggcg accaggcggg
      601 cgacaggtct tcgcccgggt cggaggtgag ccgcaaggcc gtgccaccat taatcaagcg
      661 gacgtacaca tccacgttat cccggtccgg gccgttccaa ctgaacgcca tctggctgcc
      721 gtccggcgag aaggtgaccc ggctctcatc acccggatag gtcgtcagcg gtactacacg
      781 cacgggcagc gactgctcgg agccggcgcg aataacccac caggcgaaaa agcccgccag
      841 aaaggcgagc acgcccaaac ccgcgtaaac ccacttgtgc cgggacggcg gtttcggagg
      901 cggcggaggc acaagcttcc ccgaatcgga ttcttcccgg atctcgtcga gggccacttt
      961 caggtcggcc atggtttgaa accgcttcgc cggctgcttg cgcaggcagc gcataattat
     1021 gcgctccagt tcgcgcggca cgtcctcacg gacctgcgtg accgggaccg gttcgcttcg
     1081 gacaatggca gcgagcgtgg ccagtctgga actgccgctg aacggcttcc gccccgtgac
     1141 catttcatac aacatcgcac cgaagctgaa gatatcggaa cgcgaatcga tgggctgccc
     1201 ctccgcctgt tcgggagaca tgtaggaaag cgtgcccagc accacgccct cgcgcgtatg
     1261 gggaagctct cccatcgccc cggagcttgc gtccgcgctc aggaaaagcc gtttggcaag
     1321 gccgaagtcc agaaccttca ccatgccacg gccggtcacc aggacgttgc gcggcttgat
     1381 atcgcggtgc acgatccccg cggagtgcgc cgccgccagc gcgtccgcaa tctgtaccgc
     1441 gtacttcagc gccgtatcgt agcggagccc gtgggagcct atcagttcgt ccagcgtttt
     1501 gccgtccacg tactccatgg cgatgaaatc cgaaccgtct tcggttccca cctcatagat
     1561 cgtgataatg ctggagtgat tcagggccga ggccgaacgg gcctcctgcg caaaacgcat
     1621 tctgccctct gtatcgagag cgttggcgtc agacaaaatc ttcaacgcca cgaaccggtc
     1681 caggtgcgtg tcacgggctt tgtacaccac gcccatcccg ccctcgccga gtttatcgac
     1741 gatttcgtaa tgtgaaatcg tccgcgcgcg catccgattc ctttcaggat accaatcacc
     1801 gtattccagc cgggtgccgg agacagcccc atctcacccg ctcgaacacg tcgcaagcgt
     1861 caggagaacc gggatcagga ggccttcagt tctactatgg tagcgccgga gccgccctcg
     1921 cttggcggcg ccggataaaa tttttcggcg tacgggctgt tggtgagaaa ctccgagatg
     1981 gcgcgtttca acacgcccat tccatgtccg tgcacgatgc gaacacgctg cacggcggcc
     2041 agcgccgcgc tatccaggaa tttgtcgatc gcatcgatgg cttcctccgc gcgcattcca
     2101 attacgttaa tttcccgctg aagcacattc caggtggggc cctgttggaa cgaaacgttt
     2161 ttggggagcc acgtcgcctc accggtttca ggcaacacct cggtgacgtc gccggccgcc
     2221 gcctgcatct tcatgaagcc gatctcgacc tcgatcatgt cgtgtggcag caggcggcgg
     2281 acccgcgcga tctcacttac tccggcgagg cgaactcgcg ctccttcttc gattcgggct
     2341 ttcggtgcaa cttgtgcctg ttgcgcttcg tcctgcgtgc tcaacaccgt tgcccggaac
     2401 tcttcttcaa attcgcgccg ggttcgggcc acttgccgct tggcttgcgc ggccgctttg
     2461 cgctgttccg cggtctggag aatccggtcg atcgtctcat gggcgtggcc ttcaaactcc
     2521 ttcatgagag cgtcgcaccg ccgctccagc tctcgcagct tggcagcctg cttctgttgc
     2581 cactccttga ccgtcgaccg ctcgcgggcc tctagcgctt cgcgctcttc ctgcaactgt
     2641 ttttcggtct ggctgacagc ctcgatgcgc ttgtggatct catccaggaa gcgggcgaca
     2701 tcgcgatcgc tgccgctcat cgacgcccgg gcgctttcga taatttccgc gggcatcccg
     2761 agacgcgcgg cgatgtccag ccctgctgat ttacccggcg cgcccaactg caaccggtaa
     2821 gtcggctcca gtgtgttctc gtcgaagccc atggacccgt tcaacacgcc ttccgtgttg
     2881 gcaccgtaaa tcttcagtgc cagcaggtgc gtcgacgcga tggcaaatgc gcccgccttg
     2941 cggaagtgct cgagaaccgc gacgcccagg gcgcctcctt cttccgggtc ggtggcgcgc
     3001 ccgagctcgt ccagcacgac gagagaatcc ggcgtcacct cttccaccat gtcgcgtatg
     3061 cgcgcgatgt gcgccgagaa tgtgctcaag ctttcctgaa tcgattgctg gtcgccgata
     3121 tcggccagca cgcgatcgaa gatgggaaac tcggcctggg cgcaggtgac cggcaagcct
     3181 gcctgggcca tcagggtaag caatcccacc gttttcaggg tgaccgtttt cccgccggtg
     3241 ttcggtccgc tgaccagcag agtgcggcag ttgtcgtcca tttttacgga gaagggcacg
     3301 gcgtaccggt gctggcgctt cagcacatct tcgagcagag gatggcgagc gtcctccaat
     3361 accaggcgcc gctccgaggg cgggctgaaa cgcggaacca cggccccgaa gtcgagcgcg
     3421 aactgcgcct tggcaaacaa cagctccagt tctccgaaaa cctgtgccgt ttgtgcgatg
     3481 gcggatgcgt gcatgcgcag ccgctccgtc atttcgcgca ggatccgatg tacttcccga
     3541 agctcttctt cggaaagccg taccagctcg ttattcaagt ggatggtctc cagcggttca
     3601 acaaacagcg tgtgtccact accgcttgaa ccatggatga ctccctctac ctttctctgc
     3661 tgcccggcga tgaggggcac gacaaaacga tcgtttcgaa tggtgacaaa ctcttcctgg
     3721 aggatccctt cctcacgatg cgcgcggagg aatcgttcga gcgattcctg tatgtttttc
     3781 cgctgccgct ccatctcccg ccgcagccgt gccagtgcga cgctggcatg atcggccacg
     3841 gtgccgtcgg ggaggatctt gcccgacagg tcggcgagaa ccggccggaa ctcgccgatt
     3901 tgcgcgcctc gcgcacccaa tcgcggaaag cgcccctcta cggccatcag caggccgcga
     3961 gcctcggcag cccgtcccag tacgtccgtc agtgcggcga tctccttggt ctccagaacc
     4021 gcgccttcga tggaaacttt gcgaatcgct atcgcggcat cgggcaagtc gaaacggata
     4081 cgaaccgcgg caccacgcgc ggccggctgc ggcttccgcg cggcctccag gtagccgatt
     4141 gcttcgccaa cttccgcatg catctcttcg agaaggagac ggtccgttcc cggagccacc
     4201 ttttccagtt ccgcgcgccc gaattcgctc gcgagatacc gcgacaggat ttggcgcaag
     4261 gactcgaatt ccagtagatc cgcgctcgag tgaatcataa aaaacgcagg ttgctccgtt
     4321 cccggaaacc tttctggatc acttccagtg tgccttcggt gagcttgtag ccggccagtt
     4381 cccgctcggt cacccagctt gcatccgaga catcgtcggc cggctgaagc tctcccgccg
     4441 caggccggca aatgtaatcg acgaggacat agtggtactc cggcgcgccc tcggcatcgt
     4501 gcataatccg ttcgaagatc tccaccacgg ccagggcttt cacgcgcagc ccggtttcct
     4561 ccagcacttc gcgctgcaca gcctcgatca gcgtttctcc gacctccacc acgccgccgg
     4621 gcagcgacca gtatccacgc agaggctcgc ggccgcgttc caccaggagg attcgattgc
     4681 gccggaaaac cagcgcgccg acaccgagga tggggcgttt cgggtatcgt ctgctgtcct
     4741 gaactttgga attgcgcggc actgttagca agcggaaacc actacggcac cgcgccgcgg
     4801 acgtccagtc gcacacggta cacagacgtt tgcgctgtaa tgtagagcgt catcagatcg
     4861 ggatctccga atgtgcagtt cgtgggacgt tcggcgattt caatggtttc aagcagcttg
     4921 ccctcggg
//
LOCUS       DASTMK010000480         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_57431, whole genome shotgun sequence.
ACCESSION   DASTMK010000480 DASTMK010000000
VERSION     DASTMK010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57431"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..800
                     /locus_tag="VFQ79_20150"
     CDS             66..800
                     /locus_tag="VFQ79_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142046.1"
                     /translation="MKRIFLLSSILMILAGAGFAQEDRVRRIFEPKEMPQYAFRSLAQ
                     LLSQVGHPDTQVVSDEVLRVIVVQGPPAKVDLLEQLVKRFNVVPPGADREGDFELTVQ
                     IIQASPGEQANGDKMTPDLQAVMKQVSGILNYKSFRLLDTVVIHGRSRAHSVFKGVLP
                     VGATSNPTPVNFEIVLENPSITGGKPPAVAVRQFRFGGSVPNVGQVQIQTGVEAPEGK
                     KIALGKVTMDTQGNSLLLVLSAKVME"
     gene            831..2294
                     /locus_tag="VFQ79_20155"
     CDS             831..2294
                     /locus_tag="VFQ79_20155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HEU0142047.1"
                     /translation="MQNYLAFDLGAESGRAILGTLAEGRLTLKELHRFPNTPVRLPTG
                     LYWDTLRLFHEMQEGLRIAGRERKLALDGIGVDTWGVDFGLVGCDGALVDNPRHYRDS
                     RNNGMLEKTFAAVPREEIFAQTGIQFMQFNSLYQLHAMKLAGAPALRVAAKLLFMPDL
                     FNYWLTGVQKAELTIASTSQFYNPIEKSWAVSLFERLGIRADILPEIVPPGTPLGPVL
                     PELADSCGTGDGTVYATAGHDTASAVAAAPGKGSDWCYISSGTWSLMGVELDAPVVNA
                     RSLELNLTNEVGAEGRIRLLKNIAGMWLVQECRRAWAAEGKHYSYDELAKMAADAPPF
                     AAVIDPDAFLEPGRMPEKIVDYCTGKRQKAPEEPGTFCRVIFESLALRYRQVLESIES
                     LLGRTFSTIHIVGGGSRNRVLNQFVADATGRRVVAGPSEATAAGNILVQAMGAGRLSG
                     LAEARECVARSFELTEFQPRNSEEWQIPYHRFTELRL"
     gene            complement(2304..4118)
                     /locus_tag="VFQ79_20160"
     CDS             complement(2304..4118)
                     /locus_tag="VFQ79_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEU0142048.1"
                     /translation="MKPVSRGFLLAAMSAAVLSAAAPAAPTVAEAQAFMEKVESTVMR
                     LSEEAGRASWVQATYITYDTEILAAQATEKSISANVAFAKEAKRFDGLKMPADLERKF
                     KLLKSSFTLPTPADPALSEELTKIVTGMESIYGKGKYCPPGAGNDPGKCLDLQEITRI
                     MANSRDAKQLLDVWTGWRTISPPMRKDYQRFVELSNQGARELGFADTGAMWRSKYDMP
                     PDAFARELDRLWEQVRPLYVSLHAYVRNRLREKYGDSVVPAKGPIPAHLLGNPWAQSW
                     DNIYPLVAPKDGDPGYNLTEILKSRNTDPLQMVRYGERFFTSLGFAPLPKTFWERSLF
                     TKPQDREVVCHASAWDLDNVDDLRIKMCIEINEEDFATIHHELGHNFYQRAYNQQPFL
                     FRDSANDGFHEAVGDTVSLSVTPEYLAKIELLKDVPPASKDIGLLLRRALEKVAFLPF
                     GLLIDQWRWKVFNGEITPDRYNQSWWELRQKYQGVAAPVARSEQDFDPGAKYHVPANV
                     PYTRYFLAHILQFQFHRALAQTAGCKDPLHRCSIYANEEAGKRLRDMLAMGLSRPWPE
                     ALEALTGQKQMDATAIMDYFAPLKKWLDEQNAGKPVGW"
BASE COUNT          824 a         1257 c         1251 g          836 t
ORIGIN      
        1 atccgcatcg caacctccga caagaacgtg atcgttctgc tggttcccga aacaagagga
       61 gagttatgaa aagaatattt ctactatcga gtatcctgat gatcctggcg ggcgcgggct
      121 ttgcccagga agatcgtgtc cgcagaatct tcgaacccaa agagatgccg caatacgcat
      181 tccggagcct cgcccaactc cttagccaag taggccaccc cgatacgcag gtggtttcag
      241 atgaagtcct gcgcgtgatc gtcgtccagg ggccaccggc gaaagtcgat ttactggagc
      301 agctcgtaaa gcgcttcaac gtagtgcctc cgggcgctga ccgggagggg gatttcgagc
      361 tgaccgtgca gatcatccag gcttcgccgg gagaacaggc aaatggcgac aagatgacac
      421 cggacctcca agctgttatg aagcaggtca gcgggatcct caactataag agcttccgct
      481 tgttggacac ggttgtcatt catgggcgct cccgagccca ctctgtattt aaaggcgttc
      541 tgccggttgg ggccacgagc aacccaactc ccgttaactt cgagatcgtg ctcgagaacc
      601 cctcgataac cggcggaaag cccccggctg tagccgtcag acaattcagg tttggcggct
      661 cggtccccaa tgttggccag gtgcagatcc agacaggagt agaggccccc gaaggtaaaa
      721 aaatagctct cggcaaagtc acgatggaca cgcagggcaa ttcacttctg ctggtgcttt
      781 cggccaaggt gatggaatga gcgatatact actcactctg ccccttgccc atgcaaaact
      841 atctcgcgtt tgacctcggc gcggagagcg gacgagcaat ccttgggacc ctcgctgaag
      901 gccgccttac actcaaggaa ttgcaccgct ttccgaacac gccggtgcgg ctccctaccg
      961 gactctactg ggatacgctg cgtctgtttc acgagatgca ggaaggtttg cgcatcgccg
     1021 ggcgggaacg caagctcgcg ctcgacggga taggggttga cacctggggc gtcgatttcg
     1081 gactcgtcgg ctgcgacggc gccctggtgg acaatccgcg ccactaccgt gattcacgaa
     1141 acaacggcat gctggagaaa accttcgccg ccgttccgcg cgaggagatt ttcgctcaaa
     1201 ccggcattca gttcatgcag ttcaattcgc tgtatcagct tcacgcgatg aagctggccg
     1261 gcgcgcccgc gctgcgggtg gcggcgaagc tgttgttcat gccggacctg ttcaactact
     1321 ggctgacggg cgttcaaaaa gccgagctta ccatcgcgag tacttcgcag ttctacaacc
     1381 ccattgaaaa gagctgggcc gtttctctgt tcgagcggct cggtatccgc gcggacatcc
     1441 ttccggagat tgtcccgccc ggcaccccgc tgggcccggt tcttccggag ttggccgata
     1501 gctgcggcac gggagacggc acggtgtacg ccacagccgg acatgacacg gcttccgcgg
     1561 tcgcggcggc gccgggcaaa ggttcggact ggtgctacat cagctccggc acatggtcgt
     1621 tgatgggcgt cgagctcgac gcgccggttg tgaacgcccg ttcgcttgaa ctgaacctga
     1681 cgaatgaagt gggcgccgag ggcaggattc ggctgctaaa aaatatcgcc ggcatgtggc
     1741 tggtgcagga gtgccggcgc gcgtgggcgg cggagggaaa gcactattcc tacgacgagt
     1801 tggcgaagat ggccgctgat gcgccgccgt tcgccgccgt catcgatccc gacgcgttcc
     1861 tggagcccgg ccggatgccc gaaaaaatcg tggactattg cacgggcaaa agacagaaag
     1921 cgcccgagga gcccggaacc ttctgccgcg tgatcttcga aagcctggcg ctgcgataca
     1981 ggcaggtgct cgaaagcatc gaatccctgc tggggcgcac gttctccacc atccacatcg
     2041 tcggaggcgg ctcgcgcaac cgtgtgctga accagtttgt cgccgacgcg accggcaggc
     2101 gcgtggtcgc gggtccgtcg gaagccacgg cggccggcaa catcctggtg caggcgatgg
     2161 gtgcgggccg tctttccgga ctcgccgaag cccgggaatg cgtcgcccga tcgtttgaac
     2221 ttaccgaatt tcagccccgc aacagcgagg aatggcagat cccctaccac cgcttcacgg
     2281 aactgcggct gtaatcgcga accctaccag cccaccggct tgccggcgtt ctgctcatcc
     2341 agccatttct tgagcggcgc gaaatagtcc ataatcgccg tggcgtccat ctgcttctgg
     2401 cccgtcaggg cctccaaagc ttccggccag gggcggctta gtcccatggc gagcatgtcg
     2461 cgcaaacgct ttccggcttc ctcgttcgcg tagatcgagc agcggtgcag cgggtccttg
     2521 catccggcgg tctgggccag cgcccggtgg aactggaact ggaggatgtg cgccagaaag
     2581 tagcgtgtgt agggcacgtt ggccggcacg tggtactttg cgcccgggtc gaaatcctgc
     2641 tcgctccgcg caaccggcgc ggcaacaccc tgatacttct gccgcagctc ccaccaggac
     2701 tggttgtagc ggtccggagt gatctctccg ttgaacacct tccagcgcca ctggtcaatc
     2761 agcagtccaa agggcaggaa cgcgaccttc tcgagagcgc gcctcagcag aagcccgatg
     2821 tccttcgaag caggcggcac gtcttttaac aactcgatct tcgccaggta ttccggagtg
     2881 acggagagcg atacggtatc gcccaccgct tcatgaaatc catcgttggc gctgtcgcga
     2941 aacaggaacg gctgctggtt gtatgcgcgt tgatagaagt tgtgcccaag ctcatgatgg
     3001 atggtggcga aatcctcttc gttgatctcg atgcacatct tgatgcggag atcgtccacg
     3061 ttatcgaggt cccaggcgct cgcatggcac accacttcgc gatcctgcgg tttcgtaaag
     3121 agcgaacgct cccaaaacgt tttgggaagc ggagcaaagc ccagcgacgt gaagaacctc
     3181 tccccatagc gaaccatctg aagcggatcg gtgttccggc ttttcaaaat ttccgtaagg
     3241 ttgtatcccg ggtcgccatc cttcggcgcc accaggggat agatgttgtc ccacgattgc
     3301 gcccagggat tgcccagcaa atgcgctgga atcggtcctt tggccggaac aacggagtca
     3361 ccgtattttt cgcgtagccg gttgcgcaca taagcgtgga gcgaaacgta aaggggcctg
     3421 acctgctccc atagccggtc gagctcgcgc gcgaacgcat caggcggcat gtcgtatttg
     3481 gatcgccaca tcgcacccgt atcggcgaaa ccgagttcgc gcgcgccttg gttgctcaac
     3541 tcgacgaacc gctgatagtc cttgcgcatg ggaggcgaga tcgtccgcca cccggtccag
     3601 acgtcgagca actgcttcgc atcccggctg ttcgccatga tgcgggtgat ttcctggagg
     3661 tcgaggcatt tgccgggatc gttcccggcg cccggcgggc agtatttacc cttcccgtaa
     3721 atgctctcca tccccgttac gatcttcgtc aactcctccg ataaagccgg gtcagcggga
     3781 gtaggcaagg tgaatgaact tttcaacagc ttgaattttc gctccagatc ggcgggcatc
     3841 ttcaagccgt cgaagcgctt ggcttccttc gcaaaggcga cgttggccga gatcgacttc
     3901 tcggtcgcct gtgccgccag gatctccgtg tcgtatgtga tgtaggtcgc ctgcacccac
     3961 gaagcgcgcc cggcttcttc ggaaaggcgc atcacggtgg actccacctt ctccataaac
     4021 gcctgcgcct ctgctacggt aggggcggcg ggggcggccg cggaaagcac ggcggcggac
     4081 atcgcggcaa gcaaaaagcc tcgcgagact ggcttcatct gttgttctcc actgctctaa
     4141 ttatggttca gtgccggtgt tcttcata
//
LOCUS       DASTMK010000481         3859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_376342, whole genome shotgun sequence.
ACCESSION   DASTMK010000481 DASTMK010000000
VERSION     DASTMK010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376342"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..456)
                     /locus_tag="VFQ79_20165"
     CDS             complement(193..456)
                     /locus_tag="VFQ79_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142049.1"
                     /translation="MLRDAGFFGDQELILIYIARKLKEALALEEVLTSAGLDYLIETD
                     TYVGGFVFRRERVGAFFYVAPEATRIAVELLARNGYEPYEPGE"
     gene            complement(463..1383)
                     /locus_tag="VFQ79_20170"
     CDS             complement(463..1383)
                     /locus_tag="VFQ79_20170"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0142050.1"
                     /translation="MALSALLHSVSYSGSWGQASLSVEDFADKAARLGYDGVLLMAKR
                     PHVSLLDYGDKERAALRSHLERHRFRRIALAGYNNFTADMEHGDIPNREIQIHYIVEL
                     ARLTRDLGGGLLRVFTGYDNPVAAYPAQWKAVVDCLRECARRAAEFGVTIGVQNHHDI
                     ACGYDTQHDLIRAVAEPNCLALFDAWAPALHGNDIEAAARKMGPITAHTTVADYQLRP
                     RYKYDPAIVNYNALTPAVQAVPMGEGFIDYKGFLTALSESGFSGSVAYEMCSPLIDGG
                     SVETLDRYASRFLEFMDGLRNRRAEPVATA"
     gene            1485..1769
                     /locus_tag="VFQ79_20175"
     CDS             1485..1769
                     /locus_tag="VFQ79_20175"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEU0142051.1"
                     /translation="MAAKCSHLDQVRVTQAAAHECEDCVKTGDRWVHLRMCLVCGHVG
                     CCDSSKNKHATRHFHSTQHPLIRSIEPGERWIWCYVDEVSPGTLDPHAQA"
     gene            complement(1952..2815)
                     /gene="larE"
                     /locus_tag="VFQ79_20180"
     CDS             complement(1952..2815)
                     /gene="larE"
                     /locus_tag="VFQ79_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEU0142052.1"
                     /translation="MALVALGPPQKLDPAVLKQKERKLFTILDGMGRVLVAYSGGTDS
                     AYLAWAASRVLGDNALAITADSASIPESHKRDAVEFARNWGIRHEFIETHEFENPDYA
                     KNDPDRCFHCKDELFTCLERVGKERGIEHIVYGVNKDDLGDYRPGQSAANMHRVQAPL
                     MEAGLTKPEIRELSRIAGLEIWDRPAAACLSSRIPYGTPVTKQTVKTVETGEEALKAL
                     GFRQFRVRFHGELVRIEIATDEMEKALTPEMARQFTAIFKPLGFHYVTLDLEGYRQGA
                     LNEVLKTKTAL"
     gene            complement(2843..>3859)
                     /locus_tag="VFQ79_20185"
     CDS             complement(2843..>3859)
                     /locus_tag="VFQ79_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEU0142053.1"
                     /translation="GDIHIEDLIQMEDVAVTVTRGGYMKRTPVDTYRRQSRGGKGRIG
                     MGTRAEDVVDHLVIASTHAYVLIFTNKGRVYWLKIYEIPDAGITGKGKNINNLINLQP
                     DEKVQAFLPVTEFVEDRYIVMVTRRGVIKKCQLSVFDNPMSRGIIALTLDEGDELLAA
                     RLTGGNDEIFLATREGIAIRFPEENVRAMGRPARGVRGIDLAETDFLVGMEVVNADAV
                     ILSIAENGFGKRTPVGSYRLQSRGGKGVINMKISRKTGKVVAILAVTEDSDVLMISQN
                     GKIIRIESNQIRQVGRSTQGVTLVRMEDGDQVAAASLVPETNGNGNGTHGDEEEPQGN
                     LPLQ"
BASE COUNT          749 a         1180 c         1105 g          825 t
ORIGIN      
        1 gttccagacc aaactccgta cggcttcgac tgatccacag cggttccggc ctcggccgcc
       61 ccacaccgct gccactctaa cccactttca ttttgttcgg cgggctgccg gcccatgggc
      121 actccgtgac cgtcgcggct cagaaacaaa ggctggactg agccgcgcgc gtgagcgagc
      181 ggttgtccgg tgctattcgc cgggctcgta aggttcgtat ccgttgcgcg ccagaagttc
      241 cactgcgata cgcgttgctt ccggcgccac atagaagaac gcgccaaccc gttcccgacg
      301 aaacacaaat ccgccaacat acgtgtctgt ttcaatgagg taatcgagac cggcgctggt
      361 tagcacttcc tctagcgcaa gagcctcttt cagtttcctg gcgatgtaga tcagaatgag
      421 ttcctgatcg ccaaagaagc cggcatcgcg gagcatcgtc tcttatgccg tcgccacggg
      481 ctctgcccgc ctgttgcgaa gcccatccat gaactccagg aatcgcgacg cataacggtc
      541 gagcgtttcc acgctaccgc cgtctatcag cggggaacac atctcgtaag ccacgctacc
      601 ggagaatccg gactcgctga gagcggtaag gaatcccttg taatcgatga acccttctcc
      661 catcggcacc gcctggacag cgggcgtgag cgcgttgtaa ttcacaattg cgggatcgta
      721 tttataacgc ggccgtagct ggtaatccgc gaccgtggta tgagcggtga tgggccccat
      781 cttgcgggct gccgcttcga tgtcgttgcc gtgcagcgcc ggcgcccacg catcgaacag
      841 ggcaagacag ttcggctcgg cgacggcacg aatcaggtcg tgctgtgtgt cgtagccaca
      901 agcgatgtcg tgatggttct gcacgccgat agtgacgccg aattccgccg cgcgccgcgc
      961 gcattcgcgc agacaatcca ccacggcttt ccactgggcc ggataggctg ctacggggtt
     1021 gtcgtatccc gtgaacacgc gcaggagacc tccgccaaga tcgcgcgtga gccgtgctag
     1081 ttcgacaatg tagtggattt gaatttcccg gttcgggatg tcgccatgct ccatatcggc
     1141 ggtgaagttg ttgtagccgg ccagggcgat gcggcggaaa cggtgccgtt cgaggtgaga
     1201 gcgaagcgcg gctcgctcct tatcgccgta atcgagcagc gaaacatggg gccgcttcgc
     1261 catcagcagc accccgtcat agcccagtct cgcggccttg tccgcgaaat cttcgaccga
     1321 tagagaagcc tgaccccagg aaccggagta gctgaccgaa tgcagtagtg cagaaagcgc
     1381 cataaagtac agtatacgcg gacgcccccg acgaggctcg cgcgcgcgaa gtgaagtccg
     1441 caacgctggc gcagtcaggt gttaacctga tcggggggac tcatatggca gcaaagtgca
     1501 gccacctcga ccaggtgcgg gttacgcaag cggcggctca cgaatgtgag gattgcgtca
     1561 agacaggaga tcgatgggtt catctgcgca tgtgtcttgt atgcgggcat gtagggtgct
     1621 gcgactcttc gaagaataaa cacgccaccc ggcatttcca ttcgacgcag cacccgctca
     1681 tacgctctat cgagccgggt gagcgctgga tatggtgtta tgtggacgaa gtcagtcccg
     1741 gcaccctaga cccccacgca caggcataac ggtggctatt ttccggtcac ggctccgaaa
     1801 cggagccacg cgcgtgagaa gctgtgaaga aattgaatta gccacggatg gacacagatg
     1861 aacacagata agtttcctga tttcaatctg tgtttatctg tgtccatccg tggctctgaa
     1921 tctttcacac cttctgaggg agcgccagag gctacagggc agtcttcgtt ttgagaactt
     1981 cgttcagcgc gccttgccgg tagccttcga ggtccagcgt gacgtagtgg aaaccgaggg
     2041 gcttgaaaat ggccgtgaac tgccgcgcca tctccggtgt caacgctttt tccatttcgt
     2101 ccgtggcgat ttcgatgcgc accaattccc catgaaaacg cacacggaac tggcggaacc
     2161 cgagcgcctt cagagcctct tcgccggtct cgacggtctt cacggtttgc tttgtaacgg
     2221 gagtgccgta gggaattcgc gagctcaggc aggctgccgc gggacggtcc catatctcca
     2281 ggcccgcgat gcgggaaagc tcgcggattt cgggtttcgt gagtccggct tccatcagag
     2341 gcgcctgcac ccggtgcatg tttgccgcgc tctggcccgg acggtaatcg cccagatcgt
     2401 ctttgttcac gccatacaca atgtgttcga tcccgcgctc tttgcccacc cgctccaggc
     2461 aagtgaacag ttcgtctttg cagtgaaagc agcggtccgg atcgttcttt gcataatccg
     2521 gattctcgaa ctcgtgcgtt tcgatgaact cgtggcggat cccccagttc cgcgcaaact
     2581 cgacggcgtc gcgcttgtgc gattccggaa tggaagcgga atcggcggta atggccagag
     2641 cgttgtcacc caggacgcgg cttgcagccc aggcgagata agcggaatcc gtgccgccgg
     2701 aataggccac cagcacgcgc cccattccgt cgaggatcgt aaacagtttc cgctctttct
     2761 gcttgagaac agccggatcg agcttctgcg gaggtcccag tgctactaaa gccataacta
     2821 aagtataccg gcccgctccc gtttactgga gaggcaggtt gccttgcggc tcctcctcat
     2881 cgccatgagt gccatttcca ttcccgttgg tttcgggaac caggctggcg gcggctacct
     2941 ggtcgccgtc ctccatgcgt accagggtga ctccctgcgt ggatctgccg acctgccgga
     3001 tctggttcga ctcaatgcgg atgattttgc cgttttgcga aatcatcagc acgtcggaat
     3061 cctccgttac cgcgaggatc gccaccacct tgccggtctt tcgggagatc ttcatgttga
     3121 tgacgccctt gccgccgcgc gattgcagcc ggtagcttcc caccggcgtg cgttttccga
     3181 agccgttttc ggcgattgaa agaatcaccg catccgcatt cacgacttcc atgccgacca
     3241 ggaagtcggt ttcagccagg tcgatgcccc ggacccctcg cgccggacgc cccatcgcgc
     3301 gcacgttctc ctcgggaaac cgtatggcga taccctcgcg cgtggcgaga aagatctcgt
     3361 cgttgccgcc ggtcaggcgc gcggccaaca gttcgtctcc ttcatccagg gtcagcgcaa
     3421 taatgccgcg cgacatcgga ttgtcgaaca ccgataactg gcatttcttg atcacgccgc
     3481 gccgggtgac catcacgatg tagcggtctt ccacgaactc cgttaccgga aggaacgcct
     3541 gcactttctc gtccggctga aggttgataa ggttgtttat gttcttgccc ttaccggtga
     3601 tgcctgcatc cgggatctcg taaatcttca accaatacac tcggcctttg tttgtgaaga
     3661 tcaacacgta agcgtgcgtg gacgcaatca ccaggtgatc caccacgtct tcggcgcgcg
     3721 tacccatccc gatgcgcccc ttgccgccgc gcgattgacg ccggtacgtg tccactggag
     3781 tccgcttcat gtatcctccg cgggtcaccg tgacggcaac gtcttccatc tgaatcaggt
     3841 cttcgatatg gatatcgcc
//
LOCUS       DASTMK010000482         5122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_440541, whole genome shotgun sequence.
ACCESSION   DASTMK010000482 DASTMK010000000
VERSION     DASTMK010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440541"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VFQ79_20190"
     CDS             complement(<1..1463)
                     /locus_tag="VFQ79_20190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142054.1"
                     /translation="MNVRLALSSAFLLLFTASAGYGQFTLRSGINGVVTDLTEAVIPE
                     AQVTLTDLDRSQTYTTQTNSEGQYSFVNLTPGRYQVSVERPGFQKAVSEAITVATQQT
                     IRADIVLQVGDVAQTMEVVAGAAPLLQTEQSVVGQVVEQDLVSKLPALGRNFTSFANL
                     APNISTFPRGNSGETWSVGGHHAIGGVNYIAGGGGDNGFYMNGFNINDNWVGSTSYAP
                     SIESISEVKVDVANFSAANGRDISTFTVVTRGGTNDLHGTAFDYFQNSALNAWDPYTR
                     AIMEPGQKKNLLQRNQFGGNLGGPVVIPKLVNGRDRAFFFLNYEGTRENRGGASAIYR
                     VPTEAERQGDFSGLLSRFPGDPNYVLWDPFSTTIDDEGDSVRLPVPNNDLRNIRRPDG
                     SPALNPNALSMLAMFPLPNGYQNPTNPADLRNYRTFAASGQKAYRLDARVDFRISQND
                     NVYVNYSRSSGLSTNTGGLIPELAGNIEDNSHLVTVN"
     gene            1663..>5122
                     /locus_tag="VFQ79_20195"
     CDS             1663..>5122
                     /locus_tag="VFQ79_20195"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0142055.1"
                     /translation="MVSMLFFALTLQPAALVVDGLPDDVFWRASKEVRLTPRQAGVPA
                     SMGGSVRLAGYSRYSCVLAQLPEAGGRISARSFGRNPIWEKDAMESPVVEDRIEFSFE
                     SEAGKRTVAINPWGAYRVDGDPADGVMAAARINRDGWTVESCVPLAPGKLRVRATRIR
                     ARRPLAPEFQWSAESEVVLAAGGSGSQVPVLKPPVLGNRDAPLEVGRVAQLPAVVANW
                     EDPAWRNVPAFQLPRNEAYPRAPRYSTNVKWMHDGKKLGLLLRLEEPEPVVARAGGRD
                     SATAGDDHFAIYLATNGSAIIEIRINSVGAIYDARIAGPRRMNSQSGWNADIEVQTDI
                     RHGAWYARINLPLQQLAAALGETGVPPEWRILLARHRARRSGEAAEVSALPALGTSTF
                     HGPLRYRAMILRDAAPDAVASAGTTWVEPPAAGLAHELAQLDSRVWSALERRKPATTR
                     MVDAQQRKRAEEAIRAERQEWEEVRTREDWERFRDTRIAAFRESVGEFPPQRPPLDVR
                     VSGRHSGRGYRLENLVYQSRPGYYVTANLYLPERAAARMPGMIIVHSQHFPKTQGELH
                     DMGELWARTGCAVLIIERPGYGERAETNTWYRQAYGSRFTFSKQLFLIGESYSGWVAW
                     DIIRAVDVLHDRPDIDRQRIILLGSVAGGGEPAGVAAALDPRITAVVPFNYDQGHIRV
                     HGDSPGQIAKQFSPWLVAASVAPRRFVRAFEFGWEGAEEADYPDLWVDGILRSQKVWG
                     FYGARENLATSQAYGLIRLSMERVSHCFSIGPQQRVELYPIFKRWFDIPLPSEEDLSI
                     LPDSQLSTNPYREAARRQEALRRRLHAELVSITPELSAQLERRSMHELAHEMGSKYLQ
                     AAHARRQSLTPEQRRQKLREELRPLLGDIAPGSAQATRKWTRSFSGAEVEALVLEVEP
                     GIQVPLLMMRPGTGKRAPVVVAVAQEGKERFLASRAEELERLIRAGVSVCLPDLRGTG
                     ETSPSDDRNRDGGVHFGLAQMEFDLAQNLAGARLKDLRTVLAYLRTRSDVDGNAISIW
                     GDSFVPSNSAELYLDEIEQEVSPEIQYRSEPLGALMALLAALYEDGVRAAAARGGIAS
                     YLTMLESAFTYTPMDAVLLGVLKVGDIADIVAATAPKPVLFEAAVDGRNVIVDAGTLG
                     RMFPAER"
BASE COUNT          969 a         1482 c         1649 g         1022 t
ORIGIN      
        1 tagttgaccg taacaaggtg cgagttgtct tcgatgttcc ccgccagctc cggaatcaga
       61 ccgccggtat tggtactgag tccgcttgag cgcgaatagt tcacgtaaac gttgtcgttc
      121 tggctgattc gaaaatctac acgggcatcc aggcggtagg ctttctggcc gctggctgca
      181 aacgtccggt aattgcgcaa gtcggctgga ttggttgggt tctgataacc gttaggcagc
      241 ggaaacatgg ccagcatcga cagcgcgttt ggattcaaag cgggtgagcc gtcggggcgc
      301 cggatgttgc gcaggtcgtt gttcggcacg ggcaagcgca cgctgtctcc ttcgtcgtcg
      361 atggttgtcg agaaggggtc ccaaagaaca tagttcggat cgccggggaa ccggctcagc
      421 aatccactga agtcgccctg ccgttcggcc tccgtcggga cacgataaat ggcactcgct
      481 ccaccgcgat tttcgcgcgt gccttcatag ttcaaaaaga agaacgccct atcgcgtccg
      541 ttgacgagtt tcggaatgac aaccggtccg cccaggttgc cgccgaactg gtttcgttgg
      601 agcaggttct ttttttgacc cggctccatg atcgccctgg tgtacggatc ccaggcgttg
      661 agcgcgctgt tctggaaata atcgaacgcc gttccatgaa ggtcgttggt tccgccgcgc
      721 gtcaccaccg tgaacgtgga gatgtcgcgc ccgttggccg ccgagaagtt cgccacatcc
      781 acctttactt ccgagatcga ctcaatcgac ggagcgtagc tcgtgctccc tacccagttg
      841 tcgttgatgt tgaatccgtt catgtagaag ccgttgtctc caccgccgcc ggcaatgtag
      901 ttgacgccgc cgatggcatg gtgaccgccc accgaccacg tctccccgct gtttccacgc
      961 ggaaaggtgc tgatgttcgg tgccaggttg gcgaaacttg tgaagttgcg gcccagggca
     1021 gggagcttcg agaccagatc ctgctccact acctggccga ccacgctctg ctcggtctga
     1081 agcaacggcg cggcgccggc taccacctcc atcgtctgcg ccacgtcgcc cacctgtagc
     1141 acaatgtcag ctcgtatcgt ctgctgggtg gctaccgtaa ttgcctccga taccgctttt
     1201 tgaaatccgg gacgctccac cgagacctgg tatcgccccg gcgtcagatt gacgaaagaa
     1261 tattgccctt ccgaattagt ttgagtggtg taggtctggc tgcgatccag gtcggtgagt
     1321 gttacctggg cttccggaat caccgcctct gttaaatccg taacaacgcc gttgatgccg
     1381 ctgcggagag tgaactggcc gtagccggca ctggcggtaa atagaagcag aaaagcgctt
     1441 gacaaggcga gacgtacatt cataggacct tctcctgaaa cgattcaatc ccttcatata
     1501 gcatctagac cggttctgtc aagaccaaaa tggggaagca gttatgaatc gattcataat
     1561 tttcactttg gcgttttggg ataagggcgc ttccgcttct ctcctccggg tcacgcttgt
     1621 ggaagaatca taagatcctg aagatctcag acgaggcgtg acatggtatc catgctgttt
     1681 ttcgcactaa ctctgcaacc ggccgccctg gtagtggatg gcctccccga cgacgttttc
     1741 tggcgggcgt cgaaggaggt acgcttaacc ccgcggcaag ccggtgtccc ggcatccatg
     1801 ggaggctcgg ttcgcctggc aggttacagc cgctactcct gcgtgctggc gcagttgccc
     1861 gaggcgggcg ggaggatctc cgcgcgatcc ttcgggcgca atcccatatg ggagaaggat
     1921 gccatggagt cccccgtggt ggaggaccgc atcgaattct ccttcgaatc tgaagcgggc
     1981 aagcgcacgg ttgctattaa cccgtgggga gcataccggg tcgacggcga tccggccgac
     2041 ggcgtgatgg ccgcggctcg catcaaccgg gatggctgga cggttgaaag ctgcgtaccg
     2101 cttgcccccg gtaaactgcg ggttcgcgca acgcgaattc gcgcgcggcg cccactggca
     2161 cccgaatttc agtggtctgc cgagtcggag gttgtgctgg cggcgggcgg ctccggctcg
     2221 caagttccgg tcttgaaacc acctgtgctg ggaaaccggg acgctccgct cgaagtggga
     2281 cgcgtggccc agttacctgc cgtggtagcg aattgggaag acccggcctg gcggaacgtg
     2341 ccggcttttc aacttccccg caatgaggcg tacccgcgcg cgccgcgata ctccacgaat
     2401 gtgaaatgga tgcacgacgg aaaaaaactc gggcttctgc tgcgcctcga ggagccggag
     2461 ccggtggtag cgcgcgcggg tgggcgcgac agcgccacag cgggcgacga tcatttcgcc
     2521 atctatctgg caaccaacgg ttcggcgatc atcgagattc gcatcaattc ggttggcgct
     2581 atttacgatg cgcgcatcgc cggaccgcgc cgcatgaatt cccagtccgg atggaacgcg
     2641 gacatcgaag tgcagaccga catccggcac ggcgcatggt atgcccgcat caatcttccg
     2701 ttgcagcagt tggcagcggc attgggtgaa accggcgtac cgccggaatg gcgcatactg
     2761 ctggcgcgtc accgtgcccg ccgttccggc gaggcggcgg aagtgagcgc gttgccggcg
     2821 ttgggcacgt cgacgtttca tggtccgctg cgttaccgcg caatgatctt gcgtgatgcc
     2881 gcgccggacg ctgtcgcaag tgccggaaca acatgggtgg aacctccggc cgccgggctc
     2941 gcgcacgagt tggcgcagct tgattcgcgg gtttggtccg ctctcgaaag gcgaaaaccg
     3001 gccacaaccc gcatggtgga cgcacagcag cggaaacgcg cggaagaagc gatccgggcg
     3061 gaacgccagg aatgggagga agtacgaacc cgcgaggatt gggaacgatt tcgcgacact
     3121 cgcatcgcag cgtttcgcga atcagtgggc gagttccctc cccaacggcc acccctcgat
     3181 gttcgcgtat ccggccggca tagcgggagg ggatacaggc tcgagaacct ggtttatcag
     3241 agccgccctg gatactacgt tacggccaac ctctatttac ccgaacgcgc cgccgcgcgt
     3301 atgccaggca tgatcatcgt tcacagccag cactttccga agacgcaggg cgaactgcat
     3361 gacatgggcg agttgtgggc acgcaccggt tgcgccgtgc tgatcattga acggccgggc
     3421 tacggcgaac gcgcggagac gaatacgtgg taccggcagg cgtacggttc gcggttcaca
     3481 ttttcaaaac aactgttcct gattggcgag agttattcgg gttgggtcgc ttgggatatt
     3541 atccgcgctg tcgacgtgct gcacgaccgg ccggatatcg accggcagcg catcatcctg
     3601 ctcgggtcgg tggcgggcgg cggagaaccc gcgggagtag cggcagcgct cgacccgcgc
     3661 attaccgcgg tggtgccgtt caactacgac cagggtcaca tccgcgtcca cggcgattct
     3721 ccagggcaga ttgcgaagca attctcgccc tggctggtgg ccgcctccgt ggcgccgcgg
     3781 cgcttcgtgc gggcgttcga gttcggatgg gaaggcgccg aagaagcgga ctatccagac
     3841 ctgtgggtcg acggtatact tcgctcgcaa aaagtgtggg gcttttacgg cgctcgcgag
     3901 aacctggcca cgagccaggc gtacgggttg atccgtctga gcatggagcg tgtgagccat
     3961 tgtttcagca tcggtccgca acaacgcgtg gaactgtatc cgatcttcaa gagatggttc
     4021 gacattccgc tgccctctga agaggatctg tcgattctgc cggactcgca attatccacg
     4081 aacccgtacc gggaagcagc gcggcggcag gaagcgctgc ggcggcgtct acatgccgaa
     4141 ttggtgagca tcacgcccga gctttccgcg caactcgagc gcagatcgat gcacgaactg
     4201 gcgcacgaga tgggttcaaa gtatttgcag gcggcgcacg cgcggcggca atcgctaact
     4261 ccggaacagc ggcgtcagaa actgcgcgag gagcttaggc cgcttctcgg cgacattgcg
     4321 ccgggttccg cacaggctac gcggaaatgg acgcgctcgt tttcgggcgc ggaagtggag
     4381 gcacttgtgc tggaggtgga gcccggcatc caggtgccgc tgctcatgat gcggccgggg
     4441 acagggaaac gtgcaccggt ggtggtagca gttgcacagg aaggcaagga gcggtttctg
     4501 gcgagccggg ccgaagagtt ggaacgcctg atccgggcgg gcgtgagcgt ttgcctgccg
     4561 gatttgcggg gcacgggcga gacctccccg tcagacgacc gcaaccgtga tggcggtgtg
     4621 catttcggac tggcgcaaat ggaattcgat ctcgctcaaa atctggcggg cgcccgtttg
     4681 aaagacctgc ggaccgttct tgcctacctg cgaacgcggt ctgatgtaga tggcaacgcg
     4741 atctcgattt ggggcgattc ctttgtgccg tcgaactccg ctgagttata tctcgacgag
     4801 atcgagcagg aagtttcgcc ggagatccag tatcgctcag agccgctggg agcgctgatg
     4861 gcgctgctgg ccgcactcta tgaagacggc gtgcgggccg ccgccgcgag aggcggaatc
     4921 gcgagctacc tgacgatgct tgagagcgcg ttcacgtata cgcccatgga cgcggtgttg
     4981 ctcggagttc tgaaggtagg cgatatcgcc gacattgtgg cggcgactgc accgaaaccg
     5041 gtactgttcg aagccgctgt ggacgggcga aacgtgattg tcgacgctgg aaccctgggc
     5101 cgtatgtttc ccgcggagcg gc
//
LOCUS       DASTMK010000483         6911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_600669, whole genome shotgun sequence.
ACCESSION   DASTMK010000483 DASTMK010000000
VERSION     DASTMK010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600669"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..353)
                     /locus_tag="VFQ79_20200"
     CDS             complement(60..353)
                     /locus_tag="VFQ79_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142056.1"
                     /translation="MANLEKHVKGLSTELMLADLCEVPGHRRSPRLIGRYTKNRKPYP
                     AGRRNPIHQKQPFLIRLQGMRATPESRERMEKRVRQINQRLEMLDTPFRLKLI"
     gene            complement(697..1140)
                     /locus_tag="VFQ79_20205"
     CDS             complement(697..1140)
                     /locus_tag="VFQ79_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142057.1"
                     /translation="MIVSDMLQGSPVVTFTIEQSHENALQAVKRALAKQNLRVPAQLD
                     ITAGLKQDLRVNLGPCVVLFVDDPVLLLEAIIFQRGSAVFMPLAISVSGDHRRTTAML
                     RSAESLIEGGAPPGLVDPLLSLHSRVLKAMDTIGQRERDTVLLTA"
     gene            1353..1790
                     /gene="rnk"
                     /locus_tag="VFQ79_20210"
     CDS             1353..1790
                     /gene="rnk"
                     /locus_tag="VFQ79_20210"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEU0142058.1"
                     /translation="MKKNRIYITKDDMDRLRRLIDGRFIPDSRDSDYLVDLEFELDRA
                     EVVEPKRVPRGVVTMNSEVRLEDLDTGEEKTYKLVFPGQSRGTENAVSVLAPIGTALL
                     GYRVGDTIEWQVPKGTRRLKVLEVLYQPEADPLVSRERTRVAG"
     gene            1834..2856
                     /locus_tag="VFQ79_20215"
     CDS             1834..2856
                     /locus_tag="VFQ79_20215"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU0142059.1"
                     /translation="MGLLSRFCVAALDLGTANTLIHVQGRGLVVNQASMVTLRESNGS
                     VEAVGQEARSVLGRTPPSLRIARPILRGTIADLNVCGSMLRHFIAKARENLLFARLHI
                     AVAVPGHSTAVERLAVIESLRDADVTSILLIDNVLAAAYGGSMDVDEPRGRMIADFGA
                     GVSEAAVLSLSGVVCSRSLAIGGDEMDAAIADYMQNRYHLLIGNRMAEEIKIRVGSAL
                     PLPEPLKITAKGRCLVQGIPRDVTVEDGEIREALAGPVSHIVRLTRETLEQAPPELSA
                     DIVETGIVLTGGMALLRNLDRRIEEECGVPVMVDPNPLESVVTGLARMSKNLRRNHWR
                     RFGNTV"
     gene            3026..3367
                     /locus_tag="VFQ79_20220"
     CDS             3026..3367
                     /locus_tag="VFQ79_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142060.1"
                     /translation="MASQDSSPKPENFRPRFTSYIQTKRNQIGPGRYHALEMVSKIND
                     GLERDGVPMTQEHFEIATGSLDNGDCLPKFVFKEHRCVMASEGRGVTYGAGRFFHSML
                     EGIAQPDKSKW"
     gene            complement(3375..5177)
                     /locus_tag="VFQ79_20225"
     CDS             complement(3375..5177)
                     /locus_tag="VFQ79_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU0142061.1"
                     /translation="MPVLLTCQAVSKTYGNQPLFEGISLTISDRDRLGIIGPNGSGKS
                     TFLKILAGLEAPDAGSVAVRKLVQLAYVPQNEAFPAGLTVEQVIRKSLEDTDLPPGEH
                     NARLATTLGMAGFENFSLRVDSLSGGWTKRLSIAQRLVRHPDILLLDEPTNHLDLEGI
                     LWLERLLAGASFACAVISHDRYFLENVATGMAEINASYPGGMFRVDGNYSEFLARKEE
                     FLHAETRKQEALATKVRRELEWLRRGPKARTTKAKARIDQAGRLMDELAEVSARRKSA
                     SAAIDFSATGRRTKALLECEEISKELGGRKLFENLSFKLSPGMRIGLVGPNGSGKTTL
                     LRMLAGEMEPDAGSIRRAELLRVVYFDQNREHLDPGTTLRRALAPEGDSVIFRGRPLH
                     VNAWARRFLFPPEHLEMPAGRLSGGEQARVLIARLMLQPADLLLLDEPTNDLDIPTLE
                     TLEESLSEFPGALVLVTHDRYMMDRISTAVIGLDGQGEALAFADYSQWEDWLASRSKP
                     PKESPPAARPRQTSAGPERKRLSYIESREWEAMEERILQAEALIEAAKTEMQQSAAAG
                     EAARLQAAYENMEASQREVDRLYARWAELEAKRQ"
     gene            5237..>6911
                     /locus_tag="VFQ79_20230"
     CDS             5237..>6911
                     /locus_tag="VFQ79_20230"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0142062.1"
                     /translation="MMYRLVPMLLAACAIHPVGAQTADLILFNGKIVTVDERFRIAEA
                     VAIAGNRIVGTGSNAEMKKLAGTAARLIDLKGKTVLPGLIDSHVHSVSASLHEFDHRI
                     PEMETVADVLKYIRSRAEALPDGQWIVVRQVFITRLRDQRFPTREELDQAAPRNPVMF
                     ATGPDASVNSLALKLSGIDKNFEITDGAPGRIERDPKTGEPTGILRSCSRFIKAENPG
                     KEPTLEDRRNRLKELLADYNSVGLTSIADRDAEDGDVELYRQLKDRGELTCRVFISLG
                     VNAQMPMEQIEARMSATAASPLHRYDNMLWLRGIKVYLDGGMLTGSAYMLKPWGVSKV
                     YSIDDPNYRGMRYIDPGKLYSIARTAMKNNLQPTAHSVGDGAVEALVDAYEEVNNELP
                     VASHRPCISHSNFMTLPAIQKMRRLGIVADLQPAWLWLDGATLVKQFGYDRLAYFQPY
                     KSLFENKVVAGGGSDHMQKIGGFRSVNPYNPFLGMWITLTRQPRWTDQSLHTEQRITR
                     EQAIRLYTINNAYLTFEEKEKGSIEKGKLADLIVLDRDILTCPVDEVKNI"
BASE COUNT         1426 a         2045 c         2043 g         1397 t
ORIGIN      
        1 aaaagagggg gagccggggt ttgacagcac cccggcggct tgcccggatg aagccaggct
       61 tatatcagct tcaatcggaa cggcgtatcc agcatctcca atcgctgatt gatctgacgc
      121 acgcgtttct ccattcgttc acgcgactcc ggcgtagcgc gcattccttg cagccgaatc
      181 agaaacggct gtttctgatg gatggggttc ctgcgcccgg cgggatacgg cttccggttc
      241 ttcgtgtatc ttccaattaa acgggggctg cgccggtggc ccggcacttc acacaagtca
      301 gccagcatga gttcggtaga gagccctttc acatgctttt cgagatttgc caactttcac
      361 ctcctccgga gttaccaatt acaacggttc gggcatagcc agtaaccgcg cgggagccgg
      421 aaatgatccc caaagcgcgg cgaccaggta ctatggcagg aaatacactc cagcaacatt
      481 ccgtaccggt ttaacgccag tacgcccagt ttcgctaagt gttctttcga aacccgggtt
      541 ggcgcgatgc gaaccatcgg ctcccagtag ctaagtgctg ccacgtttcc ctcactttct
      601 tttattcgag cggcggcacg cctctgcata agacatttcg cgcagccgct cgtcattcaa
      661 attgcacgtt agaggccaga gaccgtacgc gagctactac gccgtgagca ggacggtatc
      721 gcgttcgcgc tgcccaatgg tgtccattgc ctttagtact cgcgaatgca ggcttagcag
      781 gggatccacc agccccggcg gagcccctcc ctctatcagc gattccgcgc tgcgcagcat
      841 tgcggtagtg cgccggtgat cgccggaaac cgaaatcgcc aacggcataa aaacagccga
      901 gccgcgctgg aatatgatcg cctccaacag cagaaccggg tcgtctacga aaagaaccac
      961 acagggcccc aggttgacgc gcaagtcctg cttgaggccc gctgttatat ccagttgcgc
     1021 cggaacgcgt agattctgtt tggctagtgc ccgcttcact gcctgtagtg cgttctcgtg
     1081 agactgctca atggtaaagg tgacgacggg cgatccttga agcatgtcac tgacgatcac
     1141 tgcaatcgcc ggtccagaac ctacacggtc acatcctcgt ttttacgtgg ttctgctgag
     1201 cataataggg cactgctgtg ccctttcgga cagttcgcgg ggccaacccc tcgccgcaat
     1261 tgcagtgtgt tcatgagaat tcggctggca gtttgattgc tctgatcaat gcttcattgg
     1321 tgtttgcaga cctggagatt aggagaagca gtatgaaaaa gaaccgaatt tacattacca
     1381 aagacgatat ggatcggtta cggcgactca tcgatggcag gttcataccg gattcgcgag
     1441 actcggatta tcttgtcgat ctcgagttcg aactggatcg cgccgaggta gttgaaccga
     1501 agcgggttcc ccgcggggtc gtcacgatga actcggaggt ccgtctggaa gatctggaca
     1561 ccggcgagga aaagacctac aaacttgttt ttcccgggca atcccgcggg acggagaacg
     1621 ccgtctcggt tctggcaccg atcggtaccg cactgctggg ctaccgcgtg ggcgacacca
     1681 ttgaatggca agttcccaaa ggcacacggc ggctgaaagt gctggaggtg ctctatcagc
     1741 cggaggcgga cccgctggta agccgggaaa ggacgcgggt tgccggttga agccacagaa
     1801 gcctggaacc ccacggcccc ggggagggct actttgggtc tgctgtcccg attctgcgta
     1861 gcggcgctcg atttaggtac tgcaaataca ctgattcacg tacagggccg cgggcttgtg
     1921 gtcaaccagg catctatggt tacgttgcgg gagagcaacg ggagcgttga ggccgttggc
     1981 caggaagccc gcagcgtact ggggcgcaca ccaccctcgc ttcggattgc ccggccgatt
     2041 ctgcggggta ccatcgccga cctgaacgtg tgcggaagca tgctccgcca tttcatagcg
     2101 aaggcacggg agaacttgct ctttgcacgt ctgcacattg cggtcgcggt gccggggcac
     2161 tccacagccg tagaacgcct ggcggtgatc gagtccctgc gtgacgcgga cgtcacttcc
     2221 atactcctga tcgacaatgt gctggccgcc gcgtatggcg gctccatgga tgtcgacgaa
     2281 ccgcgaggca ggatgatcgc cgacttcggc gccggggtgt cggaggcggc agtactatcg
     2341 ctttcgggag ttgtctgttc gcgaagcctt gcgatcggtg gtgacgaaat ggacgccgcg
     2401 atcgccgatt acatgcaaaa ccgctaccac ctgctgattg gcaatcgcat ggcggaggag
     2461 atcaagatcc gggtgggctc ggcgctccct ctgccggaac cgctgaaaat tactgcgaaa
     2521 gggcgatgtc ttgtacaggg aattccccgg gacgtgaccg tggaagacgg cgagattcgc
     2581 gaggcgcttg ccggtcctgt cagccacatt gtcagactca cccgtgagac cctggagcag
     2641 gcacctccgg aactgagcgc ggatattgtc gaaacaggta tcgtcctgac gggaggcatg
     2701 gcgctgctcc gaaacctcga caggcgcatc gaggaggaat gcggagtgcc cgtcatggtg
     2761 gatccgaacc cactcgaatc ggtcgtcaca ggtttggcca gaatgtcgaa aaatctccgc
     2821 cgcaaccact ggcgccgctt cggaaatacc gtctaatttc tccttacttg ctcttctttt
     2881 tcaaacagac cctcagtact cccggcgttg acgccccggg atgcggtgtg ataaaaagct
     2941 ggatattcga atactgggca aggtttcctg gcgattcgat cctaccgcgc cgggcctttg
     3001 cggaaacaag gagaaaactt cagttatggc atcgcaggat tcatccccga aacctgaaaa
     3061 tttccggccg cgattcactt cctatattca gaccaagcgc aatcagattg gtccgggaag
     3121 atatcatgcg ctcgaaatgg tctcgaaaat caacgatggc ctcgagagag atggtgtgcc
     3181 gatgacacag gagcacttcg agattgccac cggctctctg gacaatggcg actgtctccc
     3241 caagttcgtt ttcaaggaac acagatgcgt catggccagc gaagggcggg gcgtgacgta
     3301 cggcgcgggg cggttttttc acagcatgct ggaagggatc gcgcaacccg acaagagcaa
     3361 gtggtagttg ccgtttattg ccgcttcgct tccagttccg cccatcgcgc gtacaggcgg
     3421 tccacctcac gttgcgaggc ttccatattt tcgtaagcag cctgcaaccg cgccgcttcg
     3481 ccggcagcgg cactctgctg catttccgtt ttcgccgcct ctatgagcgc ttcggcttgc
     3541 aggatgcgtt cttccatggc ctcccactcc cgcgactcga tgtaggaaag gcgcttccgt
     3601 tccggccccg cggaggtttg tctggggcgg gccgccggcg ggctctcctt tggtggtttc
     3661 gaacgcgaag ccagccaatc ttcccactgg ctgtagtcgg caaacgccag ggcttccccc
     3721 tggccgtcca ggccgataac ggccgtcgat atacggtcca tcatgtagcg gtcgtgcgtc
     3781 accagaacca gcgcgcccgg aaactccgac aggctctctt ccagcgtttc gagggtgggg
     3841 atgtcgaggt cgttcgtcgg ttcgtccagc agcagcaggt cggctggctg gagcatcagc
     3901 cgcgcgatga gaacgcgagc ctgctcgccg cctgaaagcc tgccagcggg catctccaga
     3961 tgctccggtg gaaacagaaa ccgcctcgcc caggcattca cgtggagcgg ccggcctctg
     4021 aatattaccg aatccccttc gggcgccagc gcccggcgca gagtggttcc ggggtcgaga
     4081 tgctcgcggt tctgatcgaa gtacacgacg cgcagaagct cggcccgccg gatcgagccg
     4141 gcatcgggct ccatctcacc ggcaagcatc cggagcagcg ttgtctttcc gctgccgttc
     4201 ggccccacaa gtccgatccg catgcccggt gaaagcttga agctcaggtt ctcgaaaagc
     4261 ttccgcccgc ccagctcttt cgagatctcc tcgcattcga gcagggcctt cgtccggcgc
     4321 cctgtcgcgc tgaaatcgat cgccgccgat gcggacttcc gccgggccga cacttccgcc
     4381 agttcgtcca tcaacctgcc ggcctgatcg atgcgtgctt tcgccttggt ggtgcgtgct
     4441 ttcggtccgc ggcggagcca ttcgagttca cggcgaacct tcgttgcgag cgcttcctgc
     4501 ttacgtgttt cggcatgcag aaactcttcc ttgcgggcca gaaactcact atagttgcca
     4561 tcgactcgaa acatgccgcc cggatacgag gcgtttatct cggccattcc cgtggcaacg
     4621 ttctccagga aataccggtc atggcttatg acggcacagg caaaggacgc gccggcgagc
     4681 agacgctcca gccagagaat cccctcgaga tcgagatgat tcgtaggctc atcgagcagc
     4741 aggatatccg gatgccggac aagacgctgg gcgatcgaaa gccgcttggt ccaaccgccc
     4801 gaaagagaat ccacccggag cgagaagttc tcgaagcctg ccatgcccaa cgtcgttgcc
     4861 agccgcgcgt tgtgctcgcc cggcggcagg tccgtgtcct ccagcgattt gcgaatgacc
     4921 tgctccacag ttaagccggc cgggaaggct tcgttctggg ggacataggc gagctgcacc
     4981 agcttgcgca ccgctacgga cccggcgtca ggcgcttcca gtcccgccag aatcttcagg
     5041 aatgtggact tgcccgaacc attcggaccg ataatgccga gccggtcgcg atcggaaatc
     5101 gtcagggaaa tcccctcaaa caagggttgg ttcccatagg ttttcgacac agcctggcat
     5161 gtgagaagaa cgggcacggt tctttcagta tcggctataa aagacggcgc cctccgcacc
     5221 cggatatgat tggggcatga tgtatcgtct tgttcctatg ctgctggcgg cctgtgctat
     5281 acatccagtg ggtgcacaaa cggctgacct gattctattc aacggaaaaa tcgtgacggt
     5341 ggacgaacgc tttcgtatcg cggaagctgt tgcgatcgcg gggaaccgca ttgtcgggac
     5401 cggtagcaac gcggagatga aaaaacttgc gggcaccgca gctcgcctca tcgatctaaa
     5461 aggcaagacg gtgctgccgg gtcttatcga ttcgcacgtg cactcggtga gtgcctcgct
     5521 tcatgagttc gatcaccgga ttcccgagat ggagaccgtg gccgatgtgc tgaagtacat
     5581 ccgctcgcgc gccgaggctt tgccggacgg acagtggatt gtggttcggc aggttttcat
     5641 cacgcggctg cgggatcagc gcttcccgac tcgtgaggaa ctcgaccagg ccgcgccgcg
     5701 caacccggtg atgtttgcaa ccgggccaga cgcttccgta aactccctcg cgctgaagct
     5761 aagcggaatc gataagaact tcgagatcac tgacggcgca ccgggccgca tcgagcggga
     5821 tcccaagaca ggcgagccca ccggcatcct gcgaagctgc agccgtttca tcaaagcaga
     5881 gaatcccggt aaagagccta cgcttgagga tcgccggaat cgtctcaaag agctgctggc
     5941 cgactacaac tcggtaggac tgacgagcat tgcggatcgg gatgccgaag acggcgatgt
     6001 cgagttatac cgccagttga aggaccgcgg cgaactgacc tgccgcgtct tcatatcgct
     6061 cggcgtcaat gcgcagatgc caatggagca gatcgaggcg cgcatgtctg ccaccgccgc
     6121 gagcccgctg caccgctacg acaatatgct gtggctgcgc ggcatcaaag tgtacctgga
     6181 tggcggcatg ttgacgggca gcgcttacat gttgaagccg tggggggtga gcaaggtcta
     6241 ctcgattgac gatccgaact accgcggcat gcgttacatc gatcccggga agctgtacag
     6301 catcgccagg acggccatga agaacaacct gcagccaacc gcgcattccg taggcgatgg
     6361 cgcggtggag gcccttgtcg acgcttacga agaagtgaac aacgaactcc ctgtagcgag
     6421 tcaccggccg tgcatctcgc actcgaattt catgacgctg cccgcgattc agaaaatgcg
     6481 ccgccttgga atcgtcgccg atctgcaacc cgcctggctt tggctggacg gagcgactct
     6541 ggtgaaacag tttggatacg accggctggc ctattttcaa ccctacaaat cgctgttcga
     6601 gaacaaagta gtggccgggg gcggatcgga tcacatgcag aagattggag gcttccgttc
     6661 ggtgaatccg tataatccat ttctcggaat gtggatcacg ctcacgcgcc agccgcgctg
     6721 gacggatcag tcgcttcaca ccgaacagcg catcacgcgc gagcaggcga tccggctgta
     6781 taccatcaat aacgcctatc tgacgttcga ggaaaaagag aagggctcca tcgaaaaagg
     6841 caagctcgcc gacctgatcg tactcgaccg cgacattctc acctgcccgg tcgacgaggt
     6901 caagaacatc g
//
LOCUS       DASTMK010000484        26769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_567384, whole genome shotgun sequence.
ACCESSION   DASTMK010000484 DASTMK010000000
VERSION     DASTMK010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 26769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26769
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567384"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFQ79_20235"
     CDS             complement(<1..237)
                     /locus_tag="VFQ79_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142063.1"
                     /translation="MILRTAVVYLAAAMIMPAAPPASEVSRPKAGEVEILKSSELKPG
                     MTGTAWTVFTGSDAEPVPVEIIGLWKNAWGPKQDI"
     gene            complement(339..956)
                     /locus_tag="VFQ79_20240"
     CDS             complement(339..956)
                     /locus_tag="VFQ79_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142064.1"
                     /translation="MARLFAILLCFASLMFSQTQGGPVTGILSPVFPPTLRQYLELTD
                     AQVSGIQAANNQYAQASQAKMARYVQVQQEISEWIAKEPLDAMAIGLRYVEQEAIRRQ
                     LQDEMKKTQAAVANLLNDAQKAKLKVLEESVRLQPLVQLAQCENLLTVDPQLRTPFPG
                     NIVPASRLNFNFGTSTGDFASFILGPAFYRGCGVTTYINPLLPLP"
     gene            complement(994..2211)
                     /locus_tag="VFQ79_20245"
     CDS             complement(994..2211)
                     /locus_tag="VFQ79_20245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEU0142065.1"
                     /translation="MTLDGLDACVRQLRSDMIDFTAELVAIASENPPGAAYEECVRAI
                     QSRLRALDLPCDTIKYRPRAGVRDQSGAAIVLSRVGKGGRALYFSGHYDVVPVTTPGQ
                     CKPVFRGNMLFGRGSADMKSGLASMLYAAAALKRLRVPLNGHVALVFVPDEETGGQRG
                     SGFLAAAGRLGANGIGMLTPEPTSGVVWNANRGALTVRVTVHGREAHIGLLHQGRNAF
                     EDAIRVVAGLQRLARRVARRRTRFRITPEAARRSLMLIGGRVEAGSNFNVVPGRCVFT
                     VDRRMNPEEDYEEERRALFAVFDEARQAGVKLDVEVLQEGHPSGTSEATALGRALSSH
                     VREVTGKTPKFELCPGLLEIRFYAEHGIPAYAYGPGLLSISHGPKEFVDTDRIVECAM
                     IYARVAATVLGAQ"
     gene            complement(2221..3051)
                     /locus_tag="VFQ79_20250"
     CDS             complement(2221..3051)
                     /locus_tag="VFQ79_20250"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0142066.1"
                     /translation="MMTQRFALGLVMCLLPGVARAETLLMPHPDDPSKNVEYFLERPS
                     GDGPWPTVVFLHGHQEAPPPGGGDFVRWGVLDRFAKHGYLAVAISQPGYGNSTGPADF
                     CGPFTQHAVSGVMSKLKGKGYISSNRVVVVGISRGALVAGLLAAYDPSIAGVVLISGL
                     YDLSSFVAHSKSPQARLIVNSILEETGGGSDALQVRSVLNYAGEIKAAVLIMNGAQDD
                     RTDPEQARRLAEKIVSQGGKARAIIYPDYGHQIPVPVREREIDTFLENATNGPGLQSH
                     "
     gene            complement(3108..3653)
                     /locus_tag="VFQ79_20255"
     CDS             complement(3108..3653)
                     /locus_tag="VFQ79_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142067.1"
                     /translation="MPAVIERRRVTLLDRGLPALPYHHDTNAMTEDEANALIARVRRS
                     IAARTSDALTRVVTELSPAYTVVALTIREPQFAELPETVAIVRKSYRLQCAADGVMYQ
                     QAICHAGRQLGLDVQMCRRGQETSRAADQLGVTPGEIEEFVSRTGRPAGPPWTEEHRR
                     AYAAGIAALAAHARRRLRIPS"
     gene            complement(3740..4078)
                     /locus_tag="VFQ79_20260"
     CDS             complement(3740..4078)
                     /locus_tag="VFQ79_20260"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEU0142068.1"
                     /translation="MAHRNIVTLLYAGLGLMFVIMSIPFILGRVEPNAYFGFRTAKTL
                     SDTRIWYEANRFMGYALLAAGAVTAGGAVFIRFLYPADPSTVVKANLGVFVLSMAVAA
                     ALSYWKLSRI"
     gene            complement(4103..5713)
                     /locus_tag="VFQ79_20265"
     CDS             complement(4103..5713)
                     /locus_tag="VFQ79_20265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0142069.1"
                     /translation="MRSLPNTFAPRQCFSYVFSLNTVEYATHWAVYEGVAAPPTPGAA
                     PLYRIHFLSESEPARVGLAGPKLRSRIELICDEYLHPRRYRVMTDSNVNIAAFEERTT
                     LYLPDGSRRELDLDPFLCILGDNIGQLDLFCRLAREELAGGFSGTFFSLGGVTTFEYR
                     LTPSEGSGWYESSFLEKLLIGEDGSLQRFEVPKDGLVAVPAEVPLPEWRNDNLLRPPL
                     EYHPPKNAGFAAEEISCPAEDVQLGATVTVPLAGGPHPVVLFIPGTGAKDRHGIAGPV
                     DIGSHEIVDYLSGQGWLGVRYDSRGTGGTELGQGFLEYGFEEAVADARAVLECVFGRP
                     DADASQVALVGHSQGALAAVMLARANPRVRALVLLAMAGRKLPEVLADQLRVQARWLG
                     HSAEQLQEQIEELDRFVEVVSTEAEWSPERVPARFYAARRHRKWYADHLARDPLAEIR
                     QVRCPALICQGAKDFQVSLEKDAQPLYQAALEAGVPVEFAAFDELDHFFKPVEGEPHV
                     SQYYVARHVSDAVKHRILEFLDRTVRTR"
     gene            5961..7136
                     /locus_tag="VFQ79_20270"
     CDS             5961..7136
                     /locus_tag="VFQ79_20270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0142070.1"
                     /translation="MRVLESRQITVLTTNTCTAACSHCCMNSGPERKGRLTFAQIRGW
                     IDEIASRTEIRTIIFAGGEPLLLGPDIFAAISHARSRRLSTRLVTNAYWALNDARARD
                     ICQKLVDAGLDELNISVDDFHLPYVDPLNVRRAFQAARTMDFQSVVVVHCTGPTTTFN
                     DEQLDALLGEQLPRLFDGERERTLFDTPGRRPFVCISNTSLQAVGRASQCLSSSDIEM
                     DERWERRSREIGGCPWAVRSPAITPDGHLVSCCGFEVRGNEVLDIGDLAGRSLTELLD
                     QADGDLPLNWIALEGPYAIMDFLKSRDPALPFHQRYGSYCELCQHIVTDPAIRKALFD
                     NMDRRVFHILQRREDIKAALEQEAAVEDWLSEPAIEPQPAGLVPERQTAVTGGHAHG"
     gene            7129..7803
                     /locus_tag="VFQ79_20275"
     CDS             7129..7803
                     /locus_tag="VFQ79_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142071.1"
                     /translation="MDKRNLANWEVLAKIRQEIVANPELRKNFYDNPTAALERAGMDL
                     DQPLIPGISGSSIRQILEENPGIVAALPETVQTGAEAALAVRVRGEEVGLALANANAN
                     ANANANANANANANANANANANANTNGFGFILETAIWQDSLREQLFADAKANLGALRM
                     SEAKVITLVDRIARDASRKTNEVKVRGGVEQTFVHRHGDFNIEVGVFENPQGLRTIRS
                     ARVIQA"
     gene            7839..9851
                     /locus_tag="VFQ79_20280"
     CDS             7839..9851
                     /locus_tag="VFQ79_20280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HEU0142072.1"
                     /translation="MGRVLLLHMPLAVPTVPNLAVELLAGVLAQSGIDADVFYGTCRL
                     ARTPAISSYIHSITGGVIFTPLYYSSETPEVVARRLIDCPKAVSELYGNFERFRAERQ
                     NAAAEWGVARRTVGSEWPFGSDCDELLADVLTHMDAARLCLDRCLQDIPVGRYDVFAF
                     SLMFDAQKLASLALARWLKEREPHAAVLFGGTACDGDMGYELLQRFPCVDAVAQGEAD
                     LTITDAVRALRGERPLESTPGLLFRFGTTITAAPPAPKLEQLDALPVPDYTSFLRQLS
                     ASDWRHDDPFILFEASRGCWWGEKHHCKFCGLRADGLAYRRKSPVRVRTEIQELASRY
                     PVHRALYSTDAILDYRALRDLMPTLPDLAARHRWKLFFELKSNLHKHEVALLAESGVT
                     TVQPGIETFSDRILALMDKGNRGIRQVQFLKWLASYGIGILYNIILGTPGETPEDYDE
                     MTRLLPYLWHLPPPASVNYLSLDKFSPYMRHPEDYGFRDVQADTVYGQLFPDPSINLS
                     RIAYKFEYRSSEMDNPQLRQAWGEFELALQAWREEHDFRQLLMRDSPSGLEIMEKDHG
                     ESTRVRLHGDAAHLYRFCDAARSFRAIRSAFPLVPESVLRLRLALWTRRRWMYASASD
                     EFLALAVEVDPPPSGVSARVAINEEPVQCSPQQLVSILTIGGEMQT"
     gene            9848..11875
                     /locus_tag="VFQ79_20285"
     CDS             9848..11875
                     /locus_tag="VFQ79_20285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HEU0142073.1"
                     /translation="MSEKVLLLTMPWATPVHPSLALGLLSAILRDHGIESECLYGNLL
                     LPRRAASSVYAIQDPGHYEDRSAGLAFAPYLYPQVTPAQIAEEVAERHLAIVGREGHL
                     ETSQWRLDVAGQATRDKLVEQTLEDIEGAGVCLERCMQRIRTGSYDIVGMSLTFETQL
                     IGSLALARRIKQFRPDIRIVLGGAACTSNQGVAMLKAFDSIDAVCLGEADAIVVPLIE
                     GLRTNPSKLANIPGIAYRGTDGVFVTRQPETIRDLDWIPRPNYDPYVEQKAASEWTGS
                     PTVLLFETSRGCWWGEKHLCSFCGLNAETLTYRSKSAQRVMQEIAGLCERYDTTDGLQ
                     AVDNIFDPRYFADLAPRLAAFQKRTPVTLFFEIKSNINLNQMYQLAASGFGTLQPGIE
                     AFNDHILSLMDKGANTIQQINFIKWAHQLGISPTYNILMRNPGETAQDYRDMIALLPY
                     IEHLPPPNGIANMQLERYSPYFLSPAKYGIRDVRPKHHYRLMFPDERVDIDELVYQFD
                     FDHDELDAPDLVAARRDFLIATLNWKAAFKVHRLIYHRWDSKILVLDRRGKSGRSEWL
                     RGVAADIFLFLDEGHSFSAIRRRFPTVPPQVLQAFLDRMVAAKYVYRHKPKDTYVMVA
                     LRVYEDPEQCRKEMDAAVEQSQARRRNRATVPPGLVALEPAFASAPSPCGS"
     gene            complement(11883..13004)
                     /locus_tag="VFQ79_20290"
     CDS             complement(11883..13004)
                     /locus_tag="VFQ79_20290"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU0142074.1"
                     /translation="MTSAQRTTTLAADIAALKNLPRVLAREGYVDAYAIYGPLNLRVD
                     LWAADLDLLPASLRVLVSAFLLGRSVARDELALVFSDQEIDALISLSILLPDGEKLHT
                     GNMVLLPLLGFLVFAQRPGVNPIAYFGEDSAALALHLMPSRGDVCLDLCTGPGIQALV
                     CSGRASRVVAVDVNPLAAAHAELNVVMNGLEDRVEIRTGDLFSAVPDLEFDFISANPP
                     LLPFPPDLPYPFVGHGGADGLDITRRILKGLPAALKPGGVCQIIGTCTGDWDAPGCEA
                     ELRELAERTGLRIRMTIPSALPLVRGAHMFDGLAWTCAAAARLDVEPVKDALEAHLRT
                     LGATHLYLFFLAISRDDRKPGLELTRHYEDHAGFWYISR"
     gene            complement(13009..13569)
                     /locus_tag="VFQ79_20295"
     CDS             complement(13009..13569)
                     /locus_tag="VFQ79_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142075.1"
                     /translation="MPVPYTLDIAQRNWILGLDPAIADVEDDALLGTGPVIRVRWKGV
                     PAAVKLFSEPSIFDNERHALEVLRGSRFVPALFAAGMADTSFSDGCSTNRFRHWICRA
                     WVEDNRRKRLPLPEPARFASELSAFCDECTKAGLSMGDGKLENFRWCGQRLTWLDYGW
                     FYPGTPAGVAAHNRAFCERLLAVLGW"
     gene            complement(13581..14828)
                     /locus_tag="VFQ79_20300"
     CDS             complement(13581..14828)
                     /locus_tag="VFQ79_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142076.1"
                     /translation="MRWRVRLAFVEIPVVDSRGVAIRAESAPPGLVSLAGRAHSAGVD
                     VELLPSTHWDAGETSLPQLEQSDAVVTWLDAFNWNAALRCVELLSKRGRPLYVLGPLL
                     DYFPEQVVSAGFQHATELASFVPRCWQDLPLPDDSALALWPRWEIGISTHEVFGFPLE
                     AQPHPANTPSRQRRSVEELAVWMQSLTGPLGSCRTPLLVADRPAEGWNAAGMDLLGEL
                     SMSVLRQHDVAPWFALRISPDDVLDEAVSSSLSLLQVKWLELLPDDMRDGESRQRFAA
                     AVRDIERAGVAGNTTVHLLAGVPGEDAHDTVASINYVAGVAAKHGITDVRCEWWYNVP
                     GSPGYIRSEPWERMFVSGTVPWFDESAPASVSGAPPGPAGRIPVNEVFELLQLLNPML
                     RLRGPSLAHIMEREKASGAEIRA"
     gene            complement(14938..15405)
                     /locus_tag="VFQ79_20305"
     CDS             complement(14938..15405)
                     /locus_tag="VFQ79_20305"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU0142077.1"
                     /translation="MTRVYRILRKPYAKKPLDGEGAYRFGGRWSSVGTRLAYTAEHLS
                     LAMIEYFIHIDPDDPPKDLVAVTAEVPDSISRISISPKLLPSDWRQSPAPPELAEIGD
                     RFVRDRRTAILIVPSALAPAESNWLVNPQHPEFAKIRLHSAEPFEYDSRFFIK"
     gene            complement(15402..15839)
                     /locus_tag="VFQ79_20310"
     CDS             complement(15402..15839)
                     /locus_tag="VFQ79_20310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEU0142078.1"
                     /translation="MVSELHAVIAELGGEPVLGRSLRTETDLQAAIREGFPQTVVEEV
                     MHAAGLTLKELATSLDLSPRSLQRRRREGRLARYESDRLYRLARIVALAKHYLGDDET
                     ATRWLRRPNRALGGCTPLQLIDTELGARTVENVLGRIAYGGVS"
     gene            complement(15905..15978)
                     /locus_tag="VFQ79_20315"
     tRNA            complement(15905..15978)
                     /locus_tag="VFQ79_20315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15944..15946),aa:Gln,seq:ctg)
     gene            complement(16058..19639)
                     /locus_tag="VFQ79_20320"
     CDS             complement(16058..19639)
                     /locus_tag="VFQ79_20320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142079.1"
                     /translation="MKLWTIGLFIACGAIVTAAHAQTGNASISGQINDPSGAVIPGAQ
                     VSIRNVATNVTHSTLSSADGRFSVTSLIPGEYTLTASFQGFRTAERGGLVLRVGDRLA
                     LDIALEVGSAADRVTVTAEVPLIRTEDAQAGLVIENRRIQDLPQYNRDPLAFVFLTPN
                     VSGSNQNDLRINGSRSKQIEYFIDGVPVTTGYLHDVPPSVPSREAIAEFKVITNGLSA
                     EYGRLSGGAVQVISRSGTNQYHGSVYEFFRNDKLNASDWNTNRFGREKGVFHDNVFGG
                     TFGGPVSIPKLYNGRDRTFFFLNYEGTRRRTGNNVALGSVPTELERQGDFSQSVLDGG
                     TPVQIFDPLTSRIDGARVRRDPFPGNRVPQSRFDALASIYIGYYPQPNRPPLPGSNNE
                     GNFIGSVSNPADNNRWTGRVDQNWSARHSTHATVSYFDSNSGNTRWYGALQPVNVTRG
                     EAYTVSLDHTVALAPSMILNLRGGVVRAQTFSGNEVDTDAGGWNLPAQVLNLLGTTSS
                     RVPNLGTGSHIMSLGGGNVNDARDTSYTGSISFQKLWSKHTFKFGWEHRRYYSNVTNG
                     GSFSLATERRVTSQFYDNPVTGHPMAGFLLGIASWGQGVQVAGPASLQAYQGAYLQDD
                     WKITPKLTINLGLRWDYEPPRTERFDRQIYWDSNYRWDIRPNEGWSWDLAQSEAGISF
                     AAPEWISQGIYGRAALMGTADYPGRTIQKNYRYNIAPRIGFAYQALPRTVVRGSYGKV
                     YLTMTGDRFLASAVDNVGFGDFARLSQDGTPDGGLTYPASFSNPLPGGLGYVPFTRDV
                     QELNYSTLGNWFVVPAVDQFPGYEHVAQLNIQREFGSGSNTWVVEVAYNGNFGRKLPF
                     FGNLHSVPNAYDVLGKTLGTNLNKQVTNPFYGQIPPNTTQGGTTNFLGRIMQVMPLWR
                     ELWAVNDPLGYSNFNSAYVQIERRFSNGFSFLSNYTFGKALHAGGGVGAEGIHNVGAI
                     GNSNGPPQANQPMKEIYGLSDYDVTHRFLFNYVLELPFGRGKRFAATTNRVINGFIGG
                     WSMAGTTLYRGGQPFSLVCASGFCRNYITIGQGKLGRPSFTEPRVAYDNNVSAHTALE
                     GSAGFTPYFNPDAFQPTTDMEIGTVGSTLHGMRGSGFSQWDFALLKNISLGMESRYLQ
                     LRFEGQNIFNQMNPGKPINSLPSRAFGTITTQEGLPRQIMIAAKFYF"
     gene            19785..20654
                     /locus_tag="VFQ79_20325"
     CDS             19785..20654
                     /locus_tag="VFQ79_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0142080.1"
                     /translation="MTDRTKNRLLLLAAAVSFSTGGALIKSSALNGWQVSGLRSGIAA
                     LALYLFLPQVRRVRNWRLLPVGFAYASMLILYVISTKLTTAANAIFLQSTAPLYLLLL
                     SPLLLHERIRRGDVVFMMSIAAGLALFFVGTEPPLASAPDPKTGNFLGAASGLAWALT
                     LAGLRWLGRSDPAGNVTLAPVLVGNALAFLVCLPMGLPLYGFGVKDAAIVLYLGIIQI
                     GLSYVLLTRAIRHVPAFEAATILLAEPALNPVWAWMVHGERPGPWPLAGGIVILTATL
                     VHTHAASRRPAAG"
     gene            complement(20674..26694)
                     /locus_tag="VFQ79_20330"
     CDS             complement(20674..26694)
                     /locus_tag="VFQ79_20330"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU0142081.1"
                     /translation="MNQRIEGTLNAQIGAKGPLGKLEVQADVKGDDLTVHQFDRLDLD
                     LTSTYSAEAQRVYVKSLLVAAPSGTIQGDGNIALNADAGRTSLNVAVRNLNLERLSRS
                     FDLPVVIASRANAQFEASWPGLEYEQAAAGVKLQIARLRPQPAENVLPVAASLTANVA
                     GRTAQVRIANLEALAAQGSGRVTVDARERLDGGVQIEVPSVRDLLGGLSGFLGRTEPL
                     AGGAIDGPATLEARFGGTIGNPQVAARLSSPGLEAGQLKEVVLDVNAAYTKDRLVLEN
                     AAIRWREQSITASGTVGLKGSTQELDLEARVADASIADVLSGLGSDIPAAGTVNATVT
                     AGGTTKSPLANVEVTASSLQAYGESFGDLIAQASLGNNLLTVSRVQLDKPDSGSFTAN
                     GTYNLESQAFTLQAEAPDFRIRQMTLPDGVPVQAEIDLSAQGSGTVNDPDVTAKLDVS
                     GLRIRDEKISDLNAAVKVANRTADLQFRAPRYQLVVDASAGIESPNPVRFEVRMEGFD
                     LATLPVKLEQPLEGTVTATVTGDGEARNWKQGQAKAEIAQLDLNWNGQPIRSDGPIRA
                     NYSGGVLTVDQAAITAADSRIAIAGAVPVEPAASPGNVTLRADVNLEGLNPFFPPDAK
                     VQARGQLTIDGQVRGTLQQIDPTATITLQNGYVAAPALDPPVENAKLNARLENGRLRL
                     QELAAQWAGGQIQATADVPLGLLPDNLPVQFPRAQGPAQLTAEVNNLQLESIGQVPEK
                     VGGRVSLRLEAQAPRPELTALTARLTFPELRLNLSGFEMNQPAPSTVVVENGTARIEQ
                     FALAGPETKIELSGTAGLDAPQPLDLKMTGDLDAAILTFFSDAIRTQGRTTLRLAVAG
                     TAADPQPSGFVQLADGQFGISTPRMQAENLNLRLDLAGREVTLSRLDGTLNGGPLAGS
                     GSLQLAGGDLENVKFGLKATEVYLEFPDDMRTVSSVNLNLNSQGREIVLGGQVTIHEG
                     SFTRQLNLDEGVLAYLQRRPQIDLTEERNRMLSRLRFQLDVDTDSPVVVDNNLARAAI
                     NVDVRLLGTYYQPGMSGRITLEEGGELHLNERTYLVERGVITFTGEQRIEPSLDILAQ
                     TKASGYDITLQIAGDPGDTKTTLTSDPPLPEPDILAVLLTGRTLEEVRGAEVDVAKEQ
                     MLSYLAGRVSGSLGRSLERSLGLSQVRIEPSLIAAEDDPTARLTVGQDITNDLKLIYS
                     MDLSNSSDQIWIGQYDITKRFQTQVVRQQDNSYRMELRHDLQFGSVSGAEQTAAQRLQ
                     RKVGNVSFQGNTDLSTQQVAGRFKVKQGNDYDFFKVRRGLDRLESFYANRDLLESRVR
                     MNRQVQDSTVDLTVNIEQGPAVQFVYEGWDVPGGVRDRIREIWKEGVFDAARAGDAMA
                     EIRNALIKDGFVRGTVGYRITFPAENRKQVLFEIQPGLRYRDVKLAFEGASGIEPSTL
                     EDLIDDQDLEIAVYTDTNKVTDLLTRYYREQGYLDAKVDAPRYELEEATRTGRVVVPI
                     TEGPLYRIGNLAFTGNSIYETEMLRREIPLQSGAVYKPDLRERSIEKLEELYQAKAYN
                     DAEFSYTTEKNTAGGVVNLVFQINENRQSVVREIVVTGNDRTSQNLIRTQVAMKPGDP
                     LTLAKLAESRRNLYNTSAFSVVYIEREDVEAPDAPQNQRPMRLLVKVHEVQPFRLRYG
                     GFFDTERGPGVIADISNRNSLGSARVLGLRTRYDSDLQEARLYFSQPLLRRFPVQTTF
                     GAFARREIIRNTDPDDTLDGSGFNTDRLGVSIQQESHLGDRYILSYGYRMENTRTYLR
                     EQDPLFPFDVRLRIAPLTATLTRDTRDDMLDATRGSFMSQAFEYAPELLGSQLRFVKY
                     FGQYFRYVPLSEPTEIPWSGIRKSRLVYAGAVRVGVGSGLGGQGLVLSERFFAGGGTN
                     MRGFQQNALGPIDALGDPLGGEAMLIVNNEIRMPLISIFDAVGFVDLGNVYRKLGDFD
                     PGDIRKTAGVGLRARTPWFLLRLDYGYKLDRRTGEPAGRLFFSIGQAF"
BASE COUNT         5269 a         8426 c         7770 g         5304 t
ORIGIN      
        1 gatgtcctgc ttcggtcccc aggcattctt ccacaaacct ataatctcga cgggcacggg
       61 ttctgcatcg ctgccggtga acactgtcca ggcagtgccc gtcatgccgg gtttcaattc
      121 agaggatttg aggatttcga cttcgcccgc tttcggacga gaaacttctg atgccggcgg
      181 cgccgcgggc ataatcatcg ctgcagcaag atacactaca gcagtccgga gaatcatggg
      241 ccttcctcga taactggtga aagtcctccc taagtcgtcg ggagggattc ttccccatta
      301 tcaaccactt tccggagccg gcacaaaccg gggcctaatt acggcaatgg aagcagcggg
      361 ttgatatacg tcgtcacacc gcaccctcga tagaacgccg gtcccaggat gaaagatgca
      421 aaatcgccgg tgctggttcc gaaattgaag ttgagacgcg atgccggaac gatgttccct
      481 ggaaacggag ttcgcaattg cggatcaacg gtcaatagat tctcgcattg cgccaactgc
      541 accagcggtt gcagccggac cgattcctcc aacaccttca gcttggcctt ttgcgcatcg
      601 ttcagaagat tggcgaccgc agcctgagtc ttcttcatct cgtcctggag ttggcggcgg
      661 atggcttcct gctctacata acgaaggcca atcgccatcg cgtccagcgg ttctttggcg
      721 atccactcgg aaatttcctg ctgaacctgg acgtaacggg ccatcttggc ttgagatgcc
      781 tgtgcgtatt ggttgttggc tgcctggatg ccggaaacct gcgcgtccgt gagttcgaga
      841 tactggcgca gagtgggcgg aaagaccgga cttaggattc ccgtcacggg accgccctgc
      901 gtttgtgaaa acataagcga agcaaagcac aacaggattg cgaacaatcg agccatgacc
      961 acagtttaca tgcgctggca ggctcctggt gcattactgc gcaccgagta ctgtagccgc
     1021 gacccgggcg tagatcatcg cgcattccac gattcggtct gtgtccacga attccttcgg
     1081 tccgtgcgag atggacagaa ggcctggtcc gtacgcgtac gcaggtattc catgctcggc
     1141 gtagaacctg atttccagca gtcccgggca cagctcgaac tttggtgttt ttccggtgac
     1201 ctcacgcaca tgactgctta gagcccgtcc gagggccgtc gcctctgacg tcccggatgg
     1261 atgcccctcc tgtagcacct cgacgtccag cttcacgccg gcttgccgcg cctcatcgaa
     1321 gactgcgaag agcgcgcggc gctcctcctc gtaatcctct tccgggttca tccgcctgtc
     1381 gacggtgaac acacagcgtc cgggaacgac gttgaagttc gagccggcct cgactcgacc
     1441 cccgatcaac atcaacgaac gccgcgcagc ctcgggcgtg atgcggaagc gcgtccggcg
     1501 ccgtgcaacc cgccgcgcca gccgctgaag gcccgccacc acacgaatgg catcttcgaa
     1561 ggcgtttcgt ccctggtgaa ggagcccgat atgtgcctcg cgtccgtgca cggtcactcg
     1621 cacggtaagc gcgccccggt ttgcgttcca gacaacaccg ctggttggtt ccggggtaag
     1681 catgccgata ccgttggcac ccaggcgccc ggccgcagcc agaaacccgg atccgcgctg
     1741 gccgccggtc tcttcatcgg gcacgaacac cagcgcgacg tggccgttca acggcacgcg
     1801 caaccgcttc aaagcggccg ccgcgtacag catgctggcc agaccgctct tcatatcagc
     1861 cgacccgcgc ccgaagagca tgttgcctcg gaacacgggc ttgcactggc caggcgttgt
     1921 taccggcacc acgtcatagt ggccggagaa gtaaagcgca cgccctccct tgccaacacg
     1981 gctcaggacg atggcggctc cggactggtc gcgaacgccc gctcgaggcc gatacttgat
     2041 cgtgtcacac gggagatcga gtgcccgaag ccgtgattgg atagcgcgta cgcattcttc
     2101 gtatgcggcg ccgggtgggt tctcggatgc gattgcgaca agctcagccg tgaagtcgat
     2161 catgtcgctc cgtagctggc ggacacacgc atcgagaccg tcaagagtca tagcgggatt
     2221 tcagtgcgat tgtaaccctg gtccatttgt ggcgttctcg aggaaggtat cgatttctct
     2281 ctcccggaca ggaacgggga tctgatggcc gtaatcggga tagatgatcg cgcgagcttt
     2341 accgccttgc gacacgattt tttcggccag acggcgggct tgctccggat cggttcgatc
     2401 gtcctgagca ccattcatga tgaggaccgc ggcctttatt tcgccggcat agttcagaac
     2461 cgaacgcact tgtagagcat cgcttccacc gcccgtctct tccaggatgg agttcacgat
     2521 cagtcttgcc tgaggcgact tcgaatgtgc cacaaaactg gacaggtcat ataggccgga
     2581 tatcaataca actcccgcaa tcgatggatc gtatgcggca aggaggcccg caaccagtgc
     2641 gccgcgactg attcccacaa cgacgactct gttggaagaa atgtagcctt tccccttcag
     2701 cttcgacatt acgccggaga ccgcatgctg cgtgaaggga ccacagaaat ctgccgggcc
     2761 cgtcgaattt ccatagccgg gctgcgagat cgcgaccgca agatatccgt gtttcgcgaa
     2821 gcggtccagt acaccccacc tcacgaaatc cccgccgcca ggtggaggcg cttcctgatg
     2881 gccgtgtagg aacactaccg taggccaagg accgtcgcct gacggcctct ccaaaaagta
     2941 ctcgacgttt ttggatggat cgtccgggtg cggcatcaga agagtctcgg cacgcgcgac
     3001 accgggtaga aggcacataa ccaaaccgag cgcgaatctc tgagtcatca ttttaaaagc
     3061 cgcaaatcgt actcgaacct tatacgatca tcgcaggcgg ctgggtgtta agacggaatc
     3121 ctgagacgcc tgcgcgcgtg agccgccagc gccgcgatgc cggctgcgta cgcccgccgg
     3181 tgctcctcgg tccacggagg accggcaggc cggccggtgc ggctgacgaa ctcctcgatc
     3241 tcgcctggcg tcacaccgag ttggtcagcg gctcgtgatg tctcctggcc gcggcggcac
     3301 atttgcacgt cgagtccgag ttgccgtccc gcgtggcaga tggcctgctg atacataacc
     3361 ccgtccgcgg cgcattgcag acgatacgat ttccttacta tagcgaccgt ttcgggaagc
     3421 tcggcaaact ggggctcacg gatcgtgagg gcgacaacag tgtaggcggg tgacagttcg
     3481 gtcacgacgc gcgtcaaagc atcggaggtg cgcgccgcga tggaccgccg gacccgtgca
     3541 atgagcgcat tggcctcgtc ctccgtcata gcgttcgtgt catggtggta gggcagcgcc
     3601 ggcaaacccc gatcgaggag tgtaactcgc ctgcgctcaa tcacggctgg cacctggtca
     3661 cgcatcccca cacaaaccac atgcgcccaa ccagtatgat ccgccacgct gacaacacac
     3721 ggtgcgccgt ggttcggtct caaatccggc tcagcttcca ataactcaac gcagcggcaa
     3781 cggccataga gagcacaaag acgcccaggt tcgccttgac gacagtcgat ggatcggcgg
     3841 gataaaggaa tcggatgaag acagcgccgc cggcggtgac cgcccccgcg gcaagcaaag
     3901 cgtaacccat gaaccggttg gcctcgtacc agattcttgt atcggacagg gtcttagcgg
     3961 tccggaagcc gaaatacgcg ttgggttcca cccggcccag aatgaacggg atgctcatta
     4021 tgacgaacat caaccccaag cccgcataca gcaacgtaac aatgttgcgg tgagccataa
     4081 ctcatgctcc agtcgtattt tatcatcggg tccgaaccgt gcggtccaga aactcgagta
     4141 tccggtgttt cactgcgtca gaaacatgcc gcgccacgta gtactgtgac acgtgcggct
     4201 cgccctccac cggcttgaag aagtggtcaa gctcgtcgaa cgcggcaaac tcgaccggca
     4261 cgccggcttc aagggcagct tgataaaggg gctgcgcgtc cttctcgagg gatacctgga
     4321 aatccttggc gccctggcag atcagcgcag gacatcgaac ctgccggatc tccgcgagcg
     4381 ggtcccgcgc caggtgatcc gcataccatt tccggtgccg ccgagcggcg tagaagcggg
     4441 caggtacgcg ttccggggac cattcggctt ccgtgctgac gacttcgaca aaacggtcga
     4501 gctcctcaat ttgctcctgc aactgttccg cggagtgtcc gagccagcgt gcctgtacgc
     4561 ggagctggtc cgcaagcacc tccggcagct tccggcccgc catggcgagc aacacgagcg
     4621 cacgcacgcg cgggtttgcg cgcgcaagca tcacggccgc cagtgctccc tgactgtgcc
     4681 ccaccagcgc cacctgggaa gcatcggcat ccggtctgcc gaacacacac tcgaggacag
     4741 ccctcgcatc ggctacggct tcttcgaagc cgtattcgag gaacccctgg ccgagttccg
     4801 ttcctccggt gccgcgtgag tcgtaccgca cgcccaacca gccttgcccg gaaagataat
     4861 cgacgatttc atggctgccg atgtccacgg gaccggcaat accatgacgg tcctttgctc
     4921 ccgtgccggg gatgaagagc acgaccgggt gcggtccgcc cgccaagggc accgtgacgg
     4981 tggcaccgag ttggacgtct tcagccgggc atgaaatctc ttcggccgcg aacccggcgt
     5041 ttttcggagg gtgatattcg agcggcggcc ggagcaggtt gtcatttcgc cactctggca
     5101 gcggcacttc cgccggcacg gcaacgagcc cgtctttcgg aacctcgaac cgctggaggg
     5161 agccatcctc tccgatcagc agcttttcca gaaacgaaga ctcataccag cccgaacctt
     5221 ccgagggcgt caggcggtat tcgaaggtcg tcacgcctcc gagtgaaaag aaggttcccg
     5281 aaaacccgcc agcgagttcc tcgcgagcca ggcggcagaa cagatcgagt tgaccgatgt
     5341 tgtcgcccag gatacagaga aacggatcca gatccagttc ccggcggctg ccgtccggaa
     5401 ggtagagagt cgtgcgctcc tcgaatgccg cgatgttgac gttggaatcg gtcataaccc
     5461 ggtaacgccg cgggtggagg tactcgtcac atatcaattc gatgcggctg cggagcttcg
     5521 gacccgccag accgacgcgc gcgggctcgc tttcactcag aaaatggatg cgatacagcg
     5581 gagcggcgcc gggagtgggg ggcgcggcga cgccttcgta cacagcccag tgtgtggcgt
     5641 attcgacggt gttcaaagag aatacgtagc tgaaacattg gcggggtgcg aaggtattcg
     5701 gcagactcct catggaactc cgttgtgcgc cgggttgcag accgcagcct gtcttcaatt
     5761 atttagcaca aaaacgaata gacgacacgc aaaaatcgca cgcgaaattg tttcgttcga
     5821 gaaagaaatc acgtgcgttt tgcgccccgg ggctcccgcc gcattggatc gaattattct
     5881 ttcgaatttc gttgacactc cggcgatgag agagtattct atacaggcac tgggctgttc
     5941 tgtattccta atatccggct atgcgggttc tcgagagccg tcaaataacg gtcctgacga
     6001 ctaatacgtg tactgccgcc tgttcccact gctgtatgaa ttccggaccc gaacgcaagg
     6061 gcaggctgac tttcgcgcag atccgcggct ggatcgatga aatcgcttcg cgaactgaaa
     6121 tccgcaccat catctttgcc ggcggcgagc cacttttgct cgggcccgac atattcgctg
     6181 ccatctccca cgcgcggtcg aggcggctca gtacacgcct ggtgacgaac gcctattggg
     6241 cgctcaacga cgcccgcgcc cgcgacatct gccagaagtt ggtggatgcc ggactcgacg
     6301 aattgaacat cagcgtcgac gacttccacc tgccctatgt cgatcccttg aacgtgcggc
     6361 gtgcgttcca ggctgcccgc acgatggatt ttcagtccgt cgtggtggtg cattgcacgg
     6421 gtcctaccac cacgttcaac gatgagcaac tcgatgcctt gcttggcgaa caactgcccc
     6481 gcctgtttga cggagaacgc gagcggacgc tgttcgatac acccgggcgg cggccgtttg
     6541 tgtgcatttc gaacacgtcg cttcaggcag tcggtagggc gagccagtgc ctttcgagct
     6601 cggatatcga aatggatgaa cggtgggagc ggcgctcgcg cgagatcggg ggatgcccct
     6661 gggctgttcg ctcccccgcc ataacaccgg acggccatct ggtgagctgc tgcggcttcg
     6721 aggtgagagg aaacgaagta ctcgatatcg gcgatcttgc cgggcgttcg ttgaccgagc
     6781 tgctcgatca ggccgacggc gatctgcctc tgaactggat cgcactcgag ggtccttacg
     6841 ccattatgga ttttctcaag tcgcgcgatc ccgctcttcc tttccaccaa cgatatggca
     6901 gctactgcga gctatgccag cacattgtta cggacccagc catccgaaag gccttattcg
     6961 acaacatgga ccggcgcgtg tttcacatcc tacagcgccg ggaagacatc aaagccgcgc
     7021 tcgagcagga agcagcagtc gaagactggc tgagcgaacc tgctattgag ccgcaacccg
     7081 ccgggcttgt gcccgaacgt cagactgctg ttacaggagg tcacgcgcat ggataaacgc
     7141 aacctggcca attgggaagt tctcgccaaa atccgccagg aaattgtcgc aaacccagaa
     7201 cttcgtaaga atttctacga taatccgacg gccgcgctcg aacgcgccgg catggatctc
     7261 gaccagccgc tgattccggg catttccggc tcgtccatcc ggcagattct cgaagagaat
     7321 ccgggcatcg tggctgccct gccggaaacg gtgcagacgg gagccgaggc cgcgctcgcc
     7381 gtgagagtcc gcggcgaaga ggtcggcctg gccctggcga atgcgaacgc caatgcgaat
     7441 gcgaatgcca acgcgaatgc gaacgccaac gcgaatgcga acgccaatgc caacgccaac
     7501 gccaacacga acggcttcgg cttcatcctg gaaaccgcca tctggcagga cagcctgcgc
     7561 gagcaattgt tcgccgacgc gaaagcgaat ctgggcgcgc tgcgtatgag cgaagcgaaa
     7621 gtcataacgc tggtggaccg catcgcccgc gatgccagcc gcaaaacgaa cgaggtgaag
     7681 gtgaggggcg gcgtcgagca aaccttcgtg caccggcacg gcgacttcaa catcgaggtt
     7741 ggagtgttcg aaaatccgca gggcctgcgg accattcgct ccgcacgagt gatacaagct
     7801 tagctgcacc gggattgttc tgcccggaca acctgccaat gggacgtgtg ctgttgctcc
     7861 atatgccact ggcagttccg acggtcccca atctcgccgt cgaactgctt gccggtgtgc
     7921 ttgcgcagtc cgggatcgac gccgatgttt tctacgggac gtgccggctg gcgcggacgc
     7981 cggccatcag ttcctatata cacagcatca ccgggggcgt tattttcacg cccctctatt
     8041 actccagcga aactccggaa gtagtggcac gccggctgat cgactgtccg aaagcggttt
     8101 cggagttata tgggaacttc gaacgcttcc gggcggagcg ccagaatgca gccgccgaat
     8161 ggggcgttgc gcgtaggact gttggatcgg aatggccctt cggctctgac tgcgacgagt
     8221 tgcttgccga cgtgctcacg cacatggacg ccgcgcggct ctgcctggat cgctgcctcc
     8281 aggacatccc ggtgggacga tacgacgttt ttgcgttctc gttgatgttc gacgcgcaaa
     8341 agcttgcttc cctggcgctg gccagatggc tgaaggagag ggagccacat gcggcggtgc
     8401 tgttcggcgg caccgcctgc gatggcgaca tgggttacga actgcttcag cgttttccct
     8461 gcgtcgatgc tgtcgcgcaa ggggaggccg acctgaccat cactgacgca gtccgcgcgc
     8521 ttcgcggcga gcggcccctc gaaagcacgc ccggactgct cttccgtttc gggaccacta
     8581 tcaccgccgc gcctcccgct cccaaacttg agcagctcga cgctctgccg gtgcccgatt
     8641 atacgagctt cctccgccag ttgtccgcga gcgactggag acacgacgat ccattcattc
     8701 tgtttgaggc ctcacgcggt tgttggtggg gcgagaagca ccactgcaag ttctgcggac
     8761 ttcgagccga cggccttgca tacaggcgga aatctcccgt gcgcgttcgt acggaaattc
     8821 aggaactcgc gtcgcgctac cccgtgcacc gggcgctcta ctccaccgac gccattctcg
     8881 actaccgcgc acttcgcgat ctcatgccaa ccttgccgga cctcgccgcg cggcaccgat
     8941 ggaagctctt ctttgaactc aagtccaacc tgcacaaaca cgaggtggcg ctgcttgctg
     9001 aatccggcgt cactaccgtc cagccgggca tagaaacttt cagcgaccgc atactcgcac
     9061 tcatggacaa gggaaatcgc ggaatccgtc aggttcaatt cctgaagtgg ctcgcctcat
     9121 acggcattgg catcctctac aacatcatcc tcggcacccc tggagaaaca cccgaagact
     9181 acgacgagat gactcgtttg cttccgtatc tctggcacct gccgccgccg gcttcggtga
     9241 actatctcag cctcgacaag ttcagcccct acatgcggca tccggaagac tatggttttc
     9301 gcgacgtgca ggccgacacg gtctacgggc aactgtttcc ggacccttct ataaatctgt
     9361 cccgaatcgc ctacaaattc gagtaccgct ccagcgagat ggacaatccg caactgcggc
     9421 aggcgtgggg cgagttcgag ctcgctctgc aagcctggcg cgaggagcac gacttcaggc
     9481 agctattgat gcgcgattct ccgtctggtc tcgagattat ggagaaagac catggtgaat
     9541 cgacccgcgt gcggctgcat ggagacgccg cccacctcta ccggttttgc gatgctgcaa
     9601 ggagcttccg cgctatccgt tccgcgttcc cgctggttcc cgaaagcgtg ctccggctcc
     9661 gcctcgctct gtggacgaga cgccggtgga tgtatgcctc cgcgtctgat gaattcctcg
     9721 ccctcgcggt ggaggtcgac ccgcctccga gtggcgtcag cgcgcgagtc gcgataaacg
     9781 aggagcccgt ccaatgcagt ccgcagcaac tcgtttccat tctgacaatc ggaggagaaa
     9841 tgcaaacatg agcgaaaagg tcctcctgct taccatgcct tgggctacgc ccgtgcatcc
     9901 ttctctcgcc ctgggactgc tgagtgcgat cctgcgcgat cacgggatcg aaagcgagtg
     9961 tctctacggc aacctgctgc tgcctcgccg ggccgcatcc agcgtctatg ccattcagga
    10021 ccccggtcat tacgaagacc gcagcgcagg gctcgctttt gcgccttacc tgtatcctca
    10081 agtcacaccc gcgcagatcg ccgaggaagt cgcggagagg cacctcgcga ttgtcggccg
    10141 ggaaggccac ctcgaaacct cacagtggcg cctggacgta gccgggcagg ccacgcgtga
    10201 caagctcgtc gagcagaccc tcgaagacat cgagggcgcg ggcgtctgcc tggagcggtg
    10261 catgcagcgg atacggacgg gaagttacga catcgtaggc atgtcgctga cttttgagac
    10321 gcaactgatc ggctcgctcg cgctcgcgcg ccgcatcaag cagttccggc cggatatccg
    10381 aatcgtcctc ggcggcgccg cctgcacgtc caaccagggc gtcgcgatgc tgaaggcgtt
    10441 cgactcgatt gatgccgtgt gcctcggtga ggccgacgcg atcgtcgtac cgctgatcga
    10501 aggacttcgc acgaacccct cgaagctcgc gaacattccg ggaatcgcat accgcggcac
    10561 ggatggagtt ttcgtcacac gccagcccga aaccatacgc gatctggatt ggattccccg
    10621 tcccaactac gacccgtacg tggaacagaa agccgcgagt gaatggaccg gctcgcccac
    10681 cgtgctactc ttcgaaacct cgcgagggtg ctggtggggc gagaaacatc tatgcagctt
    10741 ctgcggtctt aatgccgaga cgctgaccta ccggagcaag agcgcgcagc gtgtcatgca
    10801 ggaaatcgcg ggtctgtgcg agcgctacga caccaccgac gggctgcaag ccgtcgacaa
    10861 cattttcgat ccccgctact ttgccgattt ggcgccgagg ctcgccgcct tccagaagcg
    10921 gacgccggtt accctgttct tcgaaatcaa atccaacatc aatctcaacc agatgtatca
    10981 gcttgccgcc agcgggttcg gcacgctgca gccaggcatc gaggcgttca acgaccacat
    11041 cctctcgctg atggacaaag gcgccaatac cattcagcag atcaacttca tcaaatgggc
    11101 gcaccagttg ggtatcagcc ccacttacaa catactcatg cgcaaccccg gcgagacggc
    11161 ccaggattat cgcgacatga tcgccctgct tccgtacatc gagcatctgc cgccgccgaa
    11221 cgggatcgcg aacatgcagc tcgaacggta tagcccgtac ttcctgagcc cggcgaagta
    11281 cggcattcgc gacgtgcgcc cgaagcacca ctaccggctg atgttcccgg atgaacgcgt
    11341 cgacatcgat gagctcgtat atcagttcga tttcgatcac gacgaactcg atgccccgga
    11401 tctcgttgcc gcacgccgcg atttcctcat tgcgacattg aactggaaag ctgctttcaa
    11461 agtccatcgg cttatctacc accgctggga ctcgaaaatc ctggtcctcg accggcgcgg
    11521 aaaatccgga cgttcggagt ggctccgcgg agtcgcagcc gacatttttc ttttcctcga
    11581 cgaaggacat tcgttcagcg cgatccgcag gcgctttccc accgtcccgc cgcaagtgtt
    11641 gcaagcgttt ctcgatcgca tggtggcggc aaagtacgta taccggcata agccgaaaga
    11701 cacctacgtg atggtcgcgc tccgcgtgta cgaagatccg gaacaatgcc ggaaggagat
    11761 ggatgccgcg gtggagcaat cgcaggcgag gcggcgcaat cgcgcgacgg tgccccccgg
    11821 gctggtcgct ctcgagccgg cgttcgcttc cgcacccagc ccctgcggtt cctgagcgca
    11881 gatcagcgcg aaatgtacca gaaccccgcg tggtcctcgt agtgtctggt caactcgagg
    11941 ccaggcttcc ggtcatctcg cgatatggcg aggaagaaca gataaaggtg cgttgcgccg
    12001 agcgtccgca ggtgcgcttc gagtgcatct ttcaccggct cgacgtcgag gcgtgccgcc
    12061 gccgcgcacg tccaggccag gccgtcgaac atgtgggcgc cgcggaccag cggcagcgcg
    12121 gacgggatgg tcatccgaat ccgcagtccc gtgcgttcgg ccagttcgcg taactcggct
    12181 tcacatcccg gtgcatccca atcgcccgtg caggtgccga tgatctgaca gactccaccg
    12241 ggcttgagcg cagccggcag tcccttcagt atccggcgcg taatatcgag tccgtcggct
    12301 ccgccgtggc ctacgaacgg gtatggcaga tccggtggaa acggcaacag aggcgggttg
    12361 gccgaaatga agtcgaattc gagatcgggc accgcggaga acagatcgcc tgtccggatc
    12421 tcgacccggt cctcgagtcc gttcatcacc acgttcaact ccgcgtgcgc ggccgcaagc
    12481 gggttcacat cgaccgcaac tacacggctg gctcgtccgc tgcacacgag tgcctgaatg
    12541 cccggccccg tacacaagtc cagacacaca tctccgcgag acggcatcag gtgcaacgcg
    12601 agcgcagccg agtcctctcc gaagtaagcg atgggattca ctccgggacg ctgtgcgaag
    12661 acgagaaacc cgagcagcgg caggagcacc atattgccgg tatgcagctt ctcgccgtcc
    12721 ggcagcagaa tgctcaagct gatgagcgca tcgatctcct gatcggagaa cacgagcgcg
    12781 agttcatcac gcgcaacgct gcgccccagc agaaaggcgg aaaccagaac ccgcagggac
    12841 gccggcagta agtccagatc cgccgcccaa agatctaccc gcagattcag cggcccatag
    12901 atggcatatg catccacgta gccttcccgc gcaagcaccc gcggcagatt cttcaacgcc
    12961 gcaatgtcgg cggctaatgt agttgtgcgc tgagcgcttg tcatcgtttc accatcccag
    13021 cactgcaagc aggcgttcgc aaaacgcgcg attatgtgcc gcaaccccgg ccggggttcc
    13081 cggatagaac catccgtaat cgagccaggt caaccgctgc ccgcaccacc tgaagttctc
    13141 aagcttgccg tcgcccatgg acaggcctgc cttcgtacac tcgtcgcaga acgccgacaa
    13201 ctccgaagcg aagcgggcag gctccggcaa cggcaatcgc ttccgcctgt tgtcctccac
    13261 ccaggccctg catatccagt gacggaaccg gttggtggag cagccatccg aaaacgaagt
    13321 atccgccatc ccggcggcga acagcgcggg cacgaaccgg ctcccgcgca gtacttcgag
    13381 cgcatgcctt tcgttatcga atatcgacgg ctcggaaaac agcttgaccg ctgccggcac
    13441 tcccttccat cgaacacgaa tcacaggacc ggtacccagc agcgcatcgt cctcgacgtc
    13501 ggcgatagcc ggatcgaggc cgaggatcca gttccgttga gcgatatcca gcgtgtacgg
    13561 caccggcatg gtttaaccgg tcaggcacga atttccgcgc cactcgcctt ctcacgttcc
    13621 ataatgtgcg ccaggctcgg accgcgcagc cgcaacatcg ggttcagaag ttggagtaac
    13681 tcgaaaacct cattcaccgg tatccggcca gccggccccg ggggcgcccc cgatacagaa
    13741 gccggcgcgc tttcgtcaaa ccatggcaca gttcccgaaa cgaacatccg ctcccacggc
    13801 tcggagcgga tgtaacccgg cgatcccggc acgttgtacc accactcaca gcgcacgtcc
    13861 gtgatgccgt gctttgccgc tactcccgcg acatagttta tggacgccac ggtgtcgtgc
    13921 gcatcttcac cgggtacgcc ggcaagaagg tgcacggttg tgttcccggc cacgccggca
    13981 cgctcgatgt cgcgcaccgc ggcggcaaac cgctgccggg actcgccatc gcgcatatca
    14041 tcgggcagca gttcgagcca cttcacttgc agcaggctca agctggacga aaccgcctcg
    14101 tccagaacat catccggcga tatccgcagt gcgaaccagg gcgctacatc gtgctgccgc
    14161 aggacagaca tgctgagctc acccagcagg tccattcccg ccgcattcca tccctccgcc
    14221 ggtctgtcgg caaccagcag cggtgtccgg cacgatccaa gcgggcccgt cagagactgc
    14281 atccagaccg caagctcctc cacactccgg cgctgccgcg acggagtgtt tgccggatgc
    14341 ggctgcgctt ccaacgggaa tccgaacacc tcatgagtgg aaatgccgat ctcccatctc
    14401 ggccagagcg ccagggcaga gtcatccggc agcggtaagt cttgccagca ccgcggaacg
    14461 aaagaagcca actccgtggc gtgctgaaag cccgccgaga ctacctgttc cggaaagtag
    14521 tcgaggagcg gccccagcac gtagagcggc cggcctcgct tcgagagcaa ctctacgcac
    14581 cggagtgctg cattccagtt gaaagcatcg agccacgtaa cgacagcatc cgattgctcc
    14641 agttggggca gggaggtttc accggcatcc cagtgggtcg atggaagcaa ttccacgtca
    14701 acaccggccg agtgcgcccg tccggccagc gacaccaacc cgggcggcgc gctttcggcc
    14761 cgaatcgcca ccccacggct gtcaacgaca ggtatctcga caaaggccag ccgcacccgc
    14821 cacctcacaa gctcgcatga gcttctatct aatatacccg cgtgagatcg aaatacaagt
    14881 attcattcgc cgccgttcac actgagaaga aggcgtggga gtactttctc ccagccatca
    14941 tttgatgaag aaccgcgaat cgtactcgaa cggttcggca gaatgcagcc ggatctttgc
    15001 aaactccgga tgctgagggt tgaccagcca gttcgattca gccggagcga gagccgaagg
    15061 aacgataaga atcgccgtac gtcgatcgcg gacgaaacga tcaccgatct cggcgagttc
    15121 cggcggcgcc ggactctggc gccagtctga tggcaacagt tttggggaaa tcgaaattcg
    15181 tgaaatgctg tccggaacct cagccgtgac cgcgacgagg tctttcggcg gatcatccgg
    15241 atcgatgtgg atgaagtatt cgatcatcgc cagcgagagg tgctcagcgg tgtatgcgag
    15301 gcgagtccct acgctcgacc agcgaccgcc aaatcgatac gcgccttctc catcgagcgg
    15361 tttcttcgcg tacggcttgc gcagaattcg gtagacccga gtcaactaac gccgccgtag
    15421 gcaatgcgtc caagcacgtt ttccaccgtg cgcgctccga gttcggtatc aatcaactgg
    15481 agcggagtac aacctccgag agcacggttc ggccggcgaa gccaacgcgt cgcggtctca
    15541 tcatcgccaa ggtaatgctt cgccaaggca acgatgcgcg cgagccgata aaggcgatcc
    15601 gattcgtacc gggccagtcg cccctcccgg cgacgtcttt ggaggctgcg tggtgaaaga
    15661 tcgaggctgg tcgccagttc cttcagggtg agcccggcgg cgtgcatcac ctcttccacg
    15721 accgtctggg gaaagccctc gcggatggca gcctggaggt ccgtctcagt ccgaagggat
    15781 cggcccagaa caggctcgcc gcccagctcc gcaatcacgg cgtggagttc gcttaccatc
    15841 ctgtcgctta ttatagcgcc aaatgtcgct actctattga aagtggctgg ggttagtcag
    15901 gatctggctg ggaggcaggg actcgaaccc cgatacgcag atccagagtc tgcagtctta
    15961 ccgttagacg acctcccaac tattttgatt ctagcatttg cggaaacact tcccgcaatg
    16021 atcgggaacg gcggcggacg acggtccgat tgctgcttca gaagtagaat tttgcagcaa
    16081 tcatgatctg ccgcggcaga ccttcctgcg tggtaatggt tccaaacgcg cggctgggca
    16141 gtgagttgat gggcttgccc ggattcatct ggttgaatat attctgccct tcaaagcgca
    16201 gttgtagata tcgcgattcc atccccaggc tgatgttctt gagcagagcg aagtcccatt
    16261 gactgaatcc ggacccgcgc atgccgtgca gcgtgctacc cacagtccca atctccatat
    16321 cggtggtggg ctggaatgca tcgggattga aatatggcgt gaatcccgcc gatccttcca
    16381 gggcggtatg cgccgaaacg ttgttgtcat acgcgacccg cggctcggta aaggatggcc
    16441 ggcccagctt gccctgtcca atcgtgatgt agttccggca gaagcccgac gcgcagacta
    16501 acgaaaacgg ttgtccgcca cggtagaggg tggtgcccgc catactccag ccgccaatga
    16561 agccattgat aacgcggttc gtggtagcgg cgaatcgttt gccacggccg aacggcaact
    16621 ccagcacgta attaaacagg aagcgatggg tgacgtcata atcggagagg ccgtagatct
    16681 ccttcatcgg ctggtttgcc tgcggcgggc cgttgctgtt cccgatggcg cctacgttgt
    16741 gtattccctc ggcgccgaca cctccccccg catgcagtgc tttgccgaac gtgtaattgg
    16801 acaggaagct gaagccgttc gagaaacgcc gttcaatttg cacgtaggcc gaattgaagt
    16861 tggaatagcc cagcgggtcg tttactgccc aaagctcacg ccacagcggc atcacctgca
    16921 tgatgcggcc caggaaatta gttgttccac cctgcgtggt atttggcgga atctgaccat
    16981 agaacgggtt cgtcacctgt ttgttcaaat ttgtgcccag cgttttgcct aagacgtcgt
    17041 acgcattggg tacactgtgc aaattgccga aaaacggcag cttgcgtccg aagttgccgt
    17101 tataggcgac ctccactacc caggtgtttg atccggaccc aaactcacgt tggatgttca
    17161 actgcgctac atgttcgtaa cccgggaact ggtccaccgc gggaactaca aaccagttgc
    17221 ccagcgtcga atagttgagc tcctgcacat cacgcgtgaa cggcacgtag cccagcccgc
    17281 ccggcagcgg attgctgaaa ctcgccggat aggtcaggcc gccatccggc gtgccgtcct
    17341 ggctcaagcg cgcaaaatcg ccgaagccga cattgtccac cgccgacgcg agaaaccggt
    17401 cgccagtcat tgtcagatac accttgccgt agctgccccg aaccaccgtt cgcgggagcg
    17461 cctggtaggc gaagccgatg cgcggtgcaa tattgtagcg gtagtttttc tggatcgtgc
    17521 ggccggggta atccgctgtg cccatcagcg ctgcgcgccc atagatccct tgtgaaatcc
    17581 actccggggc ggcaaagctg atgccggcct cgctctgtgc caagtcccat gaccagcctt
    17641 cgttcggtct aatatcccaa cggtagttgg aatcccaata gatctgccgg tcgaagcgct
    17701 ccgtccgggg cggttcataa tcccaccgca ggcccaggtt gattgtcagc ttgggcgtta
    17761 ttttccaatc gtcctgaaga taggcgccct ggtaggcctg tagcgacgcc ggccccgcga
    17821 cttgcactcc ctggccccat gatgcaatac ccaacaggaa accggccatc ggatgcccgg
    17881 tcaccgggtt gtcatagaac tgcgatgtta cacgccgctc cgtcgccagg ctgaagctgc
    17941 cgccattggt cacattcgag taataacggc ggtgttccca gccgaacttg aacgtgtgct
    18001 tgctccacag cttctgaaaa gagatggatc cggtgtagct ggtatcgcgg gcgtcattca
    18061 catttccgcc gccaagcgac atgatgtggc tgcctgtgcc gagattcggc acccggctac
    18121 tggtggttcc cagcaggttg aggacttgcg ccggcaggtt ccagccgcct gcatcggtat
    18181 ccacctcgtt gccgctaaac gtctgggcgc gcaccacgcc cccgcggagg ttcagaatca
    18241 tcgatggtgc taacgccacc gtgtgatcga gtgaaacggt gtacgcttcc cctctggtca
    18301 cgttgacagg ttgcagcgca ccataccagc gcgtgtttcc cgagttgctg tcgaagtaag
    18361 agaccgtcgc gtgcgtcgaa tgccgcgcac tccagttctg atccactcgc cccgtccagc
    18421 ggttgttgtc ggccgggttc gaaacggacc cgatgaaatt gccctcgttg ttcgagccgg
    18481 gcagcggcgg gcgattgggt tggggatagt atccaatgta gatactcgcg agcgcgtcaa
    18541 accgggactg gggcacgcgg tttccaggaa acggatcgcg gcgcacgcgc gctccgtcaa
    18601 tccgggaagt gagcggatcg aagatctgca ccggagttcc accgtccagc acgctttggg
    18661 agaagtcccc ttgccgttcc aactcggtcg gcacggagcc cagcgccacg ttgttgcccg
    18721 tccgccgccg cgtgccttcg taattcagaa agaagaatgt cctgtcacgg ccgttataaa
    18781 gtttcggaat actcaccggc ccgccgaagg tgccgccgaa cacgttgtcg tgaaatacac
    18841 ccttctccct cccgaatcga ttggtattcc agtcagacgc gttcagcttg tcgttccgga
    18901 agaactcgta taccgaacca tggtattggt ttgttccgga ccggctgatt acctgcaccg
    18961 cgccgcccga aagacgccca tattcggccg acaacccgtt agtgatcacc ttgaattccg
    19021 cgatggcctc gcgcgacggc accgacggcg gcacatcatg cagatagccc gtggtcaccg
    19081 gaacgccatc gataaaatac tcgatctgtt tgctgcggct tccgttgatc cgcaggtcat
    19141 tctggttgct gccggagacg ttcggggtca gaaatacgaa agccagagga tcgcggttat
    19201 actgcggcaa gtcctgaata cgcctattct cgattaccag tcccgcctgt gcgtcttcgg
    19261 tccggatgag cggcacctcg gccgtcacag tcacccgatc ggccgcggag cctacctcaa
    19321 gcgcgatatc aagtgcgagc cggtcgccga cccgcagcac caagccgccg cgctccgcgg
    19381 tgcggaatcc ctggaaggag gctgttaggg tgtattcacc cggaatcagg ctggtaacac
    19441 taaatcgccc gtcggcgctc gatagcgttg agtgcgtgac attcgtcgcc acattgcgaa
    19501 tcgacacctg cgcgcccggt atcaccgcgc ccgatggatc gtttatctgg ccggaaatgc
    19561 tggcatttcc agtttgcgcg tgcgccgctg ttactattgc gccacaggca ataaacaggc
    19621 cgatcgtcca cagcttcatc gcatagctcc tctatattcc gtgaataggt tcgatatttc
    19681 acggaatgcg ttaagctggg ctttcccagt tccggaaagt atatacccaa tttgatgaaa
    19741 cttgtattga attatgcgct gtatcacacg gcgcggcctg ccgcatgaca gaccggacga
    19801 agaaccgttt actgctgctt gccgccgcgg tttcgttctc gaccggcgga gcgctcatca
    19861 agtcgagcgc gctgaatggg tggcaggtct ccggtttacg gtcgggtatc gcggcgctgg
    19921 cgctgtatct attcctgccg caggtccggc gtgtccggaa ctggcgcctg cttccggtgg
    19981 ggttcgccta cgcgtcgatg ctcatcctgt atgtgatatc gacgaagctg actacagcgg
    20041 cgaatgctat cttcctgcaa tccacggcgc cgttgtatct gctgctgctc agtcccctgc
    20101 tgctccacga acgaatccgg cgcggtgacg tcgttttcat gatgtcgatt gccgccggtc
    20161 tggcattgtt cttcgtaggc acggaacctc cgctggccag tgccccggac cccaaaaccg
    20221 gcaattttct gggagcggct tcaggtttgg cgtgggcact gacactcgcc ggtctccgtt
    20281 ggctggggcg cagcgatccg gcaggcaacg tcacgctcgc gccggttttg gtgggcaatg
    20341 ccctcgcatt tctggtttgc ctgcccatgg gactccctct ttacgggttc ggagtgaaag
    20401 acgcggccat cgtgctgtat ttgggaatca tccaaatcgg cctttcctat gttttgctga
    20461 cgcgcgccat tcggcatgtg cctgccttcg aggccgcgac cattctgctc gccgaacccg
    20521 cgttgaatcc cgtttgggcc tggatggttc acggagagcg tccggggccc tggccgctgg
    20581 ccggaggaat cgtgatcctg acggcaacgt tggtgcacac gcacgccgcg tcgcggagac
    20641 cggccgccgg ttgaaagcgg acgaccgctt tcgtcagaac gcctggccga tgctgaagaa
    20701 gagacgtccg gcgggctcgc cggttctgcg atcaagtttg tatccgtaat ccaggcgcag
    20761 caggaaccac ggggtgcggg cacgcagccc cacaccggcc gtcttgcgta tgtcgccggg
    20821 gtcgaaatcg cccaacttgc ggtacacgtt tccgaggtcc acaaatccga ctgcgtcgaa
    20881 aatgctgatg agcggcatcc ggatctcgtt gttgacaatc aacatcgcct ccccacccag
    20941 cgggtctccc agagcgtcga tcgggcccag tgcattctgt tgaaatcccc gcatgttggt
    21001 tccgcctccg gcaaagaaac gttcgctcag aaccaggccc tgcccgccca gtccgctgcc
    21061 caccccaacg cgcactgctc cggcataaac aagccgcgat ttccggatcc cgctccacgg
    21121 aatctcagtt ggttcactca gcgggacgta gcgaaaatac tggccgaagt atttaacgaa
    21181 tcgtagttgg gatcccagca actccggtgc atattcgaag gcctgcgaca tgaaggagcc
    21241 ccttgtcgca tcgagcatat cgtcgcgcgt gtcacgcgtc agggtcgccg tcagcggtgc
    21301 tattcgaaga cgaacatcaa acggaaacag cggatcctgt tctcgcaggt aagtcctggt
    21361 attctccatc cggtaaccgt agctgaggat gtaccggtcc cccaggtggc tctcctgctg
    21421 aatgctgaca ccaagccggt ccgtgttgaa tccggatcca tcaagcgtgt cgtccgggtc
    21481 cgtgttgcga atgatttcgc gtctggcaaa cgcgccgaat gtcgtctgca ccgggaaccg
    21541 ccggagcagg ggctggctga aatacagccg cgcttcctga agatcgctgt cataacgtgt
    21601 ccgcagtcca agcacacgcg cgctcccgag cgaattgcgg ttcgatatat cggcgattac
    21661 accggggccc cgttccgtat cgaagaatcc gccgtagcgc agacggaacg gctggacttc
    21721 gtgaaccttc accagcagcc gcatcggacg ttgattctga ggggcatccg gagcctcaac
    21781 gtcttcacgc tcgatgtaaa cgacggagaa ggcgctcgta ttgtagagat tgcggcgcga
    21841 ctcggccaac ttagccagcg tcagcggatc tcccggcttc atggctacct gggtgcgaat
    21901 caggttctgg cttgtacggt cgtttcctgt aaccacgatt tcccggacca cggactggcg
    21961 attctcgttg atttgaaata ccagattcac cactccgccc gcggtattct tttccgtggt
    22021 gtaagaaaac tccgcatcgt tgtaggcctt ggcctggtag agttcctcca gcttttcaat
    22081 cgacctctcg cgcaggtccg gcttgtacac cgcgccgctc tggagcggga tctcccgccg
    22141 gagcatctca gtttcgtaaa tagaatttcc ggtgaatgcc aggttcccga ttcgatacag
    22201 cggaccttcc gtaatgggaa cgacgactcg cccggtacgg gtagcttcct ccagctcata
    22261 acgcggcgca tcgaccttgg catccagata gccctgctcg cggtagtaac gtgtcaacag
    22321 gtccgttacc ttattcgtgt ccgtgtaaac agcgatttcc agatcctggt cgtcgatcag
    22381 gtcctcaagc gttgagggtt caataccgct ggcgccctcg aatgccagtt tcacgtcgcg
    22441 ataacgcaag ccaggttgga tctcgaacaa cacttgtttc cggttctccg ccggaaacgt
    22501 gatacggtag ccgaccgtgc cgcggacaaa gccgtccttg atgagagcgt tccggatctc
    22561 ggccatcgcg tcgccggcgc gggccgcatc gaacacgccc tctttccaga tctcacgtat
    22621 gcggtcccgc acgcctcccg gaacgtccca cccctcatac acgaattgca ctgccggacc
    22681 ctgttcgata ttcaccgtaa ggtccaccgt cgaatcctga acctggcggt tcatacggac
    22741 tcgcgactcc agcagatccc ggttcgcata gaagctctcc aggcgatcga gtcctctccg
    22801 gactttgaaa aaatcgtagt cgttcccctg tttgaccttg aacctgccgg cgacttgctg
    22861 cgtggagaga tccgtgttgc cctggaacga tacgttccca actttacgtt gcagccgttg
    22921 cgcggctgtt tgctccgctc cggacacact gccgaactgg agatcgtggc ggagttccat
    22981 acggtagctg ttgtcctgct gtctcacaac ctgggtctgg aaccgcttgg tgatatcgta
    23041 ttgaccgatc cagatctggt ccgagctgtt ggacaggtcc atggaataga taagtttcag
    23101 atcgttcgta atgtcctgcc ccacggtgag gcgtgccgtc ggatcgtctt cggcggcgat
    23161 caggctcggc tcgatacgca cctggctcag gccgagagat cgttcgaggc tgcggcccag
    23221 cgagccgctt actcgacccg caaggtagga aagcatctgc tccttggcga cgtccacttc
    23281 ggctccgcgc acctcctcca gcgttcgacc ggtcagcagc accgccagga tgtcgggttc
    23341 gggcagcggt gggtcggaag tgagagtcgt cttcgtgtcg ccgggatcgc cggcgatctg
    23401 aagcgtgatg tcgtagccgc tggctttggt ctgggccagg atgtccaggg acggctcgat
    23461 gcgctgctca ccggtaaatg taatgacgcc ccgttccacg agataggtgc gttcattcag
    23521 atgcagctcg ccgccttcct ccagggtgat gcgccccgac atcccgggct ggtagtacgt
    23581 tccgagcaac cggacatcga cattgatggc cgctctcgcc agattgttat cgacaaccac
    23641 cggcgaatcc gtgtcgacat cgagttgaaa ccgcaggcgc gataacatcc ggttgcgctc
    23701 ttccgtcaag tcgatctgcg gcctgcgctg aaggtacgcg agcacgccct catcgaggtt
    23761 gagttgcctg gtaaaagatc cctcgtggat ggtcacctgg ccgcccaata caatttcccg
    23821 gccctgcgaa ttcaggttga ggttgacgct ggagaccgtt ctcatgtcgt cgggaaattc
    23881 caggtagacc tcggtcgctt tcagaccgaa tttcacgttt tcgagatcgc ccccggcgag
    23941 ttgaaggctt cccgaccctg cgagcggccc tccattcaag gtgccgtcca ggcgcgacag
    24001 cgttacctct ctgcccgcca gatccaaacg caaattcaga ttctccgcct gcatgcgcgg
    24061 cgtggagatt ccaaattggc cgtcggccag ttgaacaaat cccgacggct gcggatccgc
    24121 cgccgtgccg gcgacagcaa gccgcaaggt tgtccggccc tgcgtccgga tggcgtcgga
    24181 gaagaaagtc agaatggcgg catcgaggtc gccggtcatc ttcagatcca gaggctgggg
    24241 agcatcgagc cctgccgttc cgcttagctc gattttggtc tcgggccctg ccaacgcgaa
    24301 ctgttcaata cgcgccgtgc cgttttccac tacaaccgtg gatggcgccg gctggttcat
    24361 ctcgaaaccg gagagattca gacgcagctc ggggaaggtc agccgtgcgg ttagcgccgt
    24421 caactccggc cgcggcgctt gcgcctccag ccgcagcgag acacggcccc caactttttc
    24481 gggtacctgc ccgatggact ccaactgtaa gttgtttacc tccgcagtca attgcgccgg
    24541 accctgcgcc cttggaaact gcacgggcaa attgtccggc agaagaccaa gaggcacgtc
    24601 ggcggtcgcc tgaatttgtc cacccgccca ctgcgccgcc aactcctgta gccgcagacg
    24661 tccgttttcc aggcgcgcat tcaacttggc attttccacc ggcgggtcga gcgccggagc
    24721 cgcgacgtag ccgttctgaa gcgtgatggt cgctgtcgga tcgatctgtt gcagtgtgcc
    24781 gcgaacctgc ccgtcgattg tcagttggcc gcgggcctgc actttcgcgt ccggcgggaa
    24841 aaaggggttg agcccttcga ggttcacgtc ggcccgaagg gtcacgttgc ccgggctggc
    24901 cgcgggctcc actggaactg ctcccgcaat cgcgatgcgc gagtccgccg cggtgatggc
    24961 cgcctggtca acggtaagga ctccaccgga atagttcgcg cgaatcggac cgtcgctccg
    25021 tatgggctgt ccgttccagt tcagatcaag ctgtgcaatc tcggctttcg cctgaccctg
    25081 tttccaattc cgcgcctcgc catctccggt gaccgtggct gtgactgtgc cttccagcgg
    25141 ctgttcgagc ttcaccggca gcgtggccag atcgaatcct tccatccgga cttcaaaccg
    25201 gaccggattc gggctctcga tgccggcgct cgcatccacg acaagctggt agcgcggtgc
    25261 gcgaaattgc aggtccgcgg ttcgattcgc caccttcacc gcggcattca aatcgctgat
    25321 tttttcatcc cggatacgga gtcccgacac gtccagcttc gcggtcacgt cgggatcgtt
    25381 cacagtaccg ctcccctgtg cggacagatc gatctcggcc tgcaccggca cgccatcggg
    25441 caaggtcatt tgccggattc ggaaatccgg cgcttcggcc tgcaacgtga atgcctgcga
    25501 ttccaggttg taggttccat tggccgtgaa gctgccgctg tcgggtttgt cgagttggac
    25561 ccgcgagacc gtcagcaggt tattcccgag cgacgcctgc gcaatcagat cgccgaagct
    25621 ctcgccgtag gcttggaggc tcgatgcggt gacctcgaca ttggcgagcg ggctctttgt
    25681 tgtgccgccg gccgttacgg ttgcgttgac cgtcccggcg gcggggatgt cacttcccaa
    25741 tcccgagagc acatcggcga tggacgcatc tgcgacgcgc gcctcgagat ccagttcctg
    25801 cgtggaacct ttgagcccca cggtgccact tgccgtgatc gactgctcgc gccagcggat
    25861 ggcggcattc tccagaacca gccggtcctt tgtataagcg gcgttcacgt cgaggacaac
    25921 ctctttcagt tggccggctt ccaacccggg tgacgagagc cgcgccgcaa cctgcggatt
    25981 accgatggtt cccccgaagc gggcctccaa tgtcgccggt ccgtcaatcg ctcctcctgc
    26041 caaaggttcc gtccgtccga ggaaaccgct taaccccccc agcaaatctc gaacacttgg
    26101 gacctctatc tgcacgccgc catccagccg ctcccgcgca tccaccgtga cccggcccga
    26161 tccttgtgcc gcgagcgctt ccaggttcgc gatcctcacc tgcgccgttc ttccggcgac
    26221 gtttgccgtc agagaagccg cgacgggcaa tacattctcg gccggctggg gacgaagtcg
    26281 cgcgatttgc agcttcacgc cagccgcggc ctgctcatac tcgagacccg gccaacttgc
    26341 ctcgaactgt gcgttcgccc ggctggcaat caccaccggc agatcgaaac tgcggctgag
    26401 gcgctccagg ttcaggttgc ggacggccac attcagcgaa gtgcggccgg cgtccgcatt
    26461 cagcgcgatg ttgccatccc cctggatggt tcccgagggc gccgccacca acaacgattt
    26521 cacgtagacg cgttgtgcct ccgctgaata ggttgatgtg agatcgagat ccagacgatc
    26581 gaactggtgc acggtgagat cgtcgccctt gacgtcagcc tgaacttcca gcttgcccag
    26641 cggacccttg gctccgatct gtgcgttgag cgtaccctcg atcctctggt tcactccagc
    26701 gaacttcgcg agcgacgcca ggtcgaggtt cgcttccagc cgcgtatcga aggacggatc
    26761 ggagaaatc
//
LOCUS       DASTMK010000485         6969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_505614, whole genome shotgun sequence.
ACCESSION   DASTMK010000485 DASTMK010000000
VERSION     DASTMK010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505614"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VFQ79_20335"
     CDS             complement(<1..1592)
                     /locus_tag="VFQ79_20335"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU0142082.1"
                     /translation="MILRFAMALLLGFTAAATGAEPLTYGRPGVIADLTKRSGRGVMM
                     ATPVDWDGDGDLDLLQDQRIYFQEGANLFRKGPLLPYLRWASNTTVADLDGDGVRDII
                     EGAWGEAEIRLLRGTGAAAPHSFRAPEVIVAPGVFAAVRPKERSPLLFVIPFVADWNG
                     DGLPDLLVGGRDIWSDYFPYLPARKIVTGRTGFGQIHGELFFLPNRGTKTAPRFPEAE
                     PLRLDGKPLTVFGICAPTAVDWDADGDLDLIVTQEAMPVLFFENTGTRTAPRLARPRT
                     LPLSYPGIYSKASVLDWDGDGLADLLVSSENGDFVFRNTGRKGEPYFSGEYETLRERN
                     PEIWAPGFATPHAVDYDGDGDLDLVVGSDDGDFRLFRNEGSRSRPVFAPPEFLTGAAG
                     EPLAVRVDIGFQPQGVVERFWAYTSPVLVDWDGDGDLDMMSGHISTAGYLYYENRGTR
                     RNPIWAAPRLLEAGGRIIRTAGHARMRPAPVDWNGDGLPDLVAVNDRHEYTLFERGTA
                     VSGKRELRAGRTLRFQSGKPVA"
     gene            complement(1589..2716)
                     /locus_tag="VFQ79_20340"
     CDS             complement(1589..2716)
                     /locus_tag="VFQ79_20340"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0142083.1"
                     /translation="MRRRTFLTGLAGLTARGFARAQESGVHFISLLPPGPGNPRNTEG
                     DFIELKDGTLLFAYTKFTGGGGDHDTASIASRTSSDGGASWSNEDRTLLENEGGMNVM
                     SVSFLRGSDGRILFFYLRKNSETDCHAMLRTSSDEGKTWGEAVDCTPDPGYFVLNNDR
                     AVELKRGRLVLPVALHTEDGKWAGRGKVMFYLSDDMGKKWRRSKAVIECPVPSKAGLQ
                     EPGVVELKDGRLMVFCRTMLGSQYVAYSRDGGETWSQAAPSALASPLSPASIERIPKT
                     GDLLAVWNDHADVDAAMRADNDAGKGGLRTPLSVAISRDEGKTWSNRKNILSDPEGWY
                     CYTAIDFWRDRVLLAYVAGGSGLPRLSRTQIAWFPVDFLYR"
     gene            complement(2720..5965)
                     /locus_tag="VFQ79_20345"
     CDS             complement(2720..5965)
                     /locus_tag="VFQ79_20345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142084.1"
                     /translation="MSQLPLRFALAVVSVSVLAVVPCLPQTTTGAISGTVRDASDAVI
                     TDAKVTVRNEETGLTRTTVTSGDGLYRVSFLPAGTYSVRVEREGFKTEVQTGIAVEIL
                     QVRSVDVRLQVGSVSDTVTVESTAPMLEAETSQAGEVIKTEQVLSLPLSARQFLQLTF
                     LVPMATPATGDFRSTEINRESPMPAAAGQRPEQNNYQIDGIDNRESGRNSLAVGASVE
                     AISEFKVQTGMAPAEFGRGGGVIINVATKTGTNNLHGSLYEFLRNDKFDARPFFSDRV
                     SPLKRNQFGGSLGGPIVRDKLFFFGNYEGFREAATGNPPVGRVMTENERNGIFATAIV
                     DPSTGAPFPNNTIPQNRLDPISRRILELVPLPNNPSDPARNFIFNGVPSGRPNRDYAV
                     GRIDYNLGAKDNIFGRYLYNKETLRTPPNLPAPANSGGRDFRLRAQSASINWNRVITP
                     SVVNSFTLGYTRYHNLLGTLNSFRQDLITPAGITNTLASTDPLFWAAPSVGVAGYTFP
                     SEVTPNYRTMNNYQLQESVLWNKGRHTIKFGGDLRQIRTNMFYTGGNGSTSFANRYTG
                     DNAADFLLGLASSVSKTARATLWNSRLTYAGAFLQDDWKVTNRLTLNIGLRYEVETAL
                     KQGDNGGLGFDVASGEMLISRQAKNIAPIEDFYRNVRPDIPYRLVDETRPYDADTNNF
                     QPRFGFAYSLHAKTVLRGGYGIYFDAPQMPSLASTNDFAPNTLRPIWTADPRIPNLGY
                     NPEAGSAAEQTLRNAPLTIFPFLSRKFPYGKIQQWNFSIQQQLSGSFVIEALYQGSSG
                     VNLLVFDNINFRTPGPGNVQSQLPYPQFARIQAEDVWGRSWFHGLGFKAEQRFYNGLS
                     YLLAYTWSKSLDLASTLNSAPLWTDPFNRGTAKGPSDFNAPHRFSAAYEYRLPFGKGR
                     PLFADASGVADKFVSGWGVRGMTFFQTGLPQSPSMALSRTGICATACSARPDRIGDGN
                     LSKDERTLQRFYDVNAFRVLAAGGADRRVGNAGRNILVSAGVNNFDLQVFKDTSIREG
                     HAIEFRWEMFNAFNHTQWGGASTNMESPQQFGIISGTRAPRIMQFVLRYAF"
     gene            complement(6059..>6969)
                     /locus_tag="VFQ79_20350"
     CDS             complement(6059..>6969)
                     /locus_tag="VFQ79_20350"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142085.1"
                     /translation="LRTGTGPLEWWSAFSEAGRTQIQFVSLDLTVRLTVIGIMLSQFF
                     WSVCTNGSDQVAVQRYLSTPSIRMARRTAWVYIAINVGLLAVLAFCGLALFGFYLQQS
                     GLPVSDFQREIAPRADALMPQFIVKELPQGVSGLVLAALLAAAMSSLSSGINSLAAVV
                     MKDLVERFGRHRTEASSLALTKLVSVAGGLIGIAAAAAITWAVRRTDWNLIDVTQRLN
                     HIFVGPLAVLFFAGILFPFASKRSVLAGFLAGTACSLYVAFGRGQSISFTWLVPASFV
                     VGFAIAAVLGRLRAEKVPAGGLAGVE"
BASE COUNT         1441 a         2151 c         2083 g         1294 t
ORIGIN      
        1 aacgctaccg gctttcccga ttgaaaacga agcgtgcgtc ccgcccgcag ttcccgcttg
       61 ccggacacgg ctgttcctcg ctcgaaaagg gtgtattcat gacgatcgtt caccgccacc
      121 agatccggca gcccgtcgcc gttccaatct accggagcgg gccgcatgcg tgcgtgcccg
      181 gcggtacgga tgatccggcc tcctgcctcc agcaggcgag gcgcggccca tatcggattc
      241 cggcgggtgc cgcgattttc gtagtacaga tacccggcgg tcgagatgtg gccgctcatc
      301 atgtccaggt ctccgtcgcc gtcccaatcg acgagcaccg gcgaagtgta ggcccaaaac
      361 ctctcgacga ctccttgcgg ctggaacccg atgtccacgc gcaccgccag cggttcgccg
      421 gccgcaccag tgagaaactc gggaggcgcg aacacgggcc gagatcgcga tccttcattg
      481 cggaacagcc ggaagtcgcc gtcgtcgcta ccgacgacca aatcgagatc gccgtcgccg
      541 tcatagtcga ctgcatgcgg cgttgcaaag cctggcgccc agatctcggg gttccgctcg
      601 cgtagcgttt catattcgcc ggagaagtac ggctcgcctt tccggccggt attgcgaaaa
      661 acgaagtcgc cgttttcact cgaaaccagc agatcggcca acccgtcgcc atcccaatcg
      721 agaacgctag ccttgctgta aatacccgga tagctcagag gcagcgtacg cggcctggcc
      781 agacggggag ccgtgcgcgt gccggtgttt tcgaagaaca gcaccggcat ggcctcctgc
      841 gtcacgatca gatcgagatc gccatcggcg tcccaatcca ccgccgttgg agcgcatatt
      901 ccgaatacgg tgagcggctt cccatcaagg cgcagtggtt cggcttccgg aaaccggggc
      961 gccgtcttcg tgccgcggtt cggaaggaaa aacaattcgc catggatctg gccgaagccg
     1021 gtcctgccgg tcacgatttt acgcgccggg agatagggaa agtaatccga ccagatatcg
     1081 cggccgccca ccagcagatc tggcaagccg tcgccattcc agtccgcaac gaaaggaata
     1141 acgaacaaca ggggcgaacg ctccttcggc cggacggccg cgaacacgcc cggtgccaca
     1201 atcacctcgg gcgcccgaaa cgaatggggc gccgcggctc ccgtgccccg aagaaggcgg
     1261 atttcggctt cgccccacgc gccttcgatg atgtcccgca ctccatcgcc atccagatcc
     1321 gcgacggttg tattggaggc ccatcgcaga tagggcagca gcggcccttt gcgaaataaa
     1381 ttcgcacctt cctgaaaata gatccgctga tcctgtaaca gatcgaggtc gccgtctccg
     1441 tcccaatcga cgggagtggc catcatgacg ccgcgcccgc tgcgtttagt gagatcggcg
     1501 ataacgccag gccggccgta ggtcagcggt tcggcgcctg tggcagccgc cgtgaagccc
     1561 aggagcagag ccatggcgaa gcgaaggatc accggtataa gaaatccacc ggaaaccatg
     1621 cgatttgcgt gcggctcaag cgaggaaggc cgctgccgcc cgccacatag gccagcaaca
     1681 cacggtcgcg ccaaaaatcg atcgcggtgt aacagtacca cccctccgga tcggacagga
     1741 tgttcttccg gttgctccac gtcttcccct cgtcgcggga gatggcaaca ctgagcggcg
     1801 tgcgaaggcc gcccttgccc gcgtcattat cggcgcgcat cgcggcatcg acatccgcgt
     1861 gatcgttcca caccgccaga agatcccccg tctttggaat ccgctcgatg gaagccggtg
     1921 agagtggtga agcgagcgca gacggcgcgg cctgcgacca cgtctccccg ccgtcgcggg
     1981 aatacgccac atactggctg cccagcatgg tccggcagaa gaccatcagg cgtccatctt
     2041 tcagctccac gacccccggc tcctgcaacc ccgctttact cggcacggga cactcgatga
     2101 cggccttact gcgcctccac ttttttccca tgtcgtcgga aagatagaac atcaccttgc
     2161 cgcggccggc ccacttgccg tcttcggtgt gcaacgcgac cggtagcacc agacggccgc
     2221 gtttcaactc cacggcccga tcgttgttga gaacgaaata gcccggatcg ggcgtgcaat
     2281 ccaccgcctc gccccacgtc ttgccttcat cggaagacgt ccgcagcatg gcatggcaat
     2341 ccgtctcaga atttttgcgc agatagaaga acagaatacg cccgtcgctc ccacgcagga
     2401 aggagacgct catcacgttc attccgcctt cattctcgag caacgtccgg tcctcattgc
     2461 tccacgaggc cccaccgtcg gaggaggtac gcgacgcgat agaggcggtg tcatgatctc
     2521 caccgcctcc ggtgaacttt gtatacgcga acagaagagt tccatccttc agttcaatga
     2581 agtcgccttc ggtattccgg gggttgcccg ggccaggcgg cagcagggaa atgaaatgga
     2641 ccccgctctc ctgcgcacgg gcaaacccgc gcgcggtcag gcccgccaat ccagttaaaa
     2701 aagtccggcg tctcatgatt caaaaagcgt atcgcagaac aaactgcatg atgcgaggcg
     2761 cgcgcgtgcc cgagataatt ccgaactgct gcggcgactc catgttggta ctggctccgc
     2821 cccattgcgt atggttaaag gcgttgaaca tctcccaacg gaactcgata gcatgccctt
     2881 cccgaatgct cgtatccttg aacacctgga gatcgaaatt attcaccccg gcgctgacca
     2941 gaatattccg cccggcgttg cctacacggc ggtcggcgcc gcctgccgca agcacgcgga
     3001 atgcgttcac gtcgtagaaa cgctgcaggg tccgctcgtc tttcgacagg ttaccgtcgc
     3061 cgatgcgatc cgggcgggcg gagcaggcgg tggcgcagat gccggtacgc gacagggcca
     3121 tggaaggaga ctgcggcagt cccgtctgga agaaagtcat accgcgaacg ccccaaccgc
     3181 tgacgaactt gtccgcaacg ccggaagcgt ccgcaaacag gggcctccct tttccgaagg
     3241 gaagccggta ttcgtaagcg gcggaaaagc ggtggggggc attgaagtcc gaagggccct
     3301 tcgccgtgcc ccggttaaaa ggatcggtcc acagcggcgc gctgttcaac gtggaggcca
     3361 ggtccagcga ttttgaccag gtgtaagcga gcaggtagct caggccgtta tagaagcgct
     3421 gttccgcttt gaatcccagg ccatggaacc agctccgccc ccacacatct tccgcctgaa
     3481 tacgggcgaa ctgcggatag ggcagttgcg actgcacgtt gcccggcccg ggcgtgcgaa
     3541 agttgatgtt atcgaacacc agcagattga ccccgctcga accctggtat agcgcttcga
     3601 tcacgaagct gcccgaaagc tgctgctgaa tactgaaatt ccactgctga attttcccgt
     3661 agggaaattt cctcgacagg aaaggaaaga tggtcagcgg cgcattgcgg agtgtttgtt
     3721 cggcggcgct tcccgcctcc gggttgtagc caaggttcgg aatgcgaggg tccgcggtcc
     3781 agatgggacg cagggtgttc ggagcgaagt cgttggtaga ggcgagcgaa ggcatctgcg
     3841 gagcgtcaaa gtagatgcca tatccaccgc gcagcaccgt cttcgcatgc agactgtaag
     3901 caaacccgaa tctcggctgg aagttattcg tgtctgcatc gtagggccgg gtttcgtcta
     3961 caagccggta aggaatgtcg gggcggacat tgcggtagaa gtcctctatc ggcgctatgt
     4021 ttttcgcctg acgcgagatc agcatctcgc cgctcgccac gtcgaagccc aacccgccgt
     4081 tatcgccctg tttgagcgct gtctccactt cataacgtag cccgatgttc agcgtcaggc
     4141 ggttggtgac tttccaatcg tcctgcaaga aagcgcccgc gtaggtcaac cgcgagttcc
     4201 acaacgtggc gcgcgccgtc ttgctcacgc tggaggccag ccccaaaaga aaatccgcgg
     4261 cgttatctcc ggtgtaccgg ttggcgaacg acgtcgaccc attgccaccc gtgtagaaca
     4321 tattcgttcg aatctggcga agatccccac cgaacttgat ggtgtgccgg cctttgttcc
     4381 acagcacact ctcctgcaac tggtaattgt tcatcgtgcg gtaattgggc gtcacttcgc
     4441 tcgggaatgt gtatcccgca accccgacac tgggggcggc ccagaacagc gggtcggtag
     4501 aggccagggt attcgtgatt ccagcgggag tgatcaggtc ctggcggaaa gaattgagag
     4561 tgcccagcag gttgtggtag cgcgtgtatc caagcgtgaa gctattgaca accgacggtg
     4621 ttataacgcg gttccagttg atgctggcgc tctgcgcccg gagtctgaaa tcacgcccgc
     4681 ccgagttcgc gggagcgggg aggtttggcg gagtgcgaag cgtttccttg ttgtacaggt
     4741 acctgccgaa aatgttgtct ttagcaccca ggttgtaatc gatccgtccg acggcataat
     4801 cgcgattcgg tcgcccggaa ggcacgccgt tgaaaatgaa attgcgtgcc gggtcggagg
     4861 ggttattggg aagcggcacc aactccagaa tgcgccggga aatcggatcg agccggtttt
     4921 gcgggatggt attgtttgga aacggcgctc cggtactcgg atccacaatg gcggttgcga
     4981 aaatgccgtt ccgctcgttc tcggtcatca cccgccccac cggaggattt cctgtggcgg
     5041 cttcgcggaa gccttcatag ttcccaaaga agaaaagctt gtcacggacg attggtccgc
     5101 ccagcgaacc tccgaactga ttgcgtttga gtgggctgac ccggtctgaa aagaacgggc
     5161 gggcatcgaa tttgtcgttg cgcagaaact cgtaaagcga gccgtgcaaa ttgttcgtac
     5221 cggtcttcgt ggccacgttg atgatgacgc cgccgccgcg tccgaactcg gcgggcgcca
     5281 tgccggtctg taccttaaat tcgctgatcg cttccacgga cgctccaacc gcgaggctgt
     5341 tccgcccgct ttcgcgattg tcgatgccat cgatctggta attgttttgt tccggccgct
     5401 gcccggcggc cgccggcatg ggggactcgc ggttgatttc cgtcgaccgg aaatcgccgg
     5461 tggcgggcgt cgccatggga accagaaagg tcagttgtaa aaactggcgg gcgctgagcg
     5521 gcaggctcaa cacctgttct gtcttgatca cttcgcccgc ttgcgaggtc tccgcttcca
     5581 gcatcggggc cgtgctttcc accgtcacgg tgtcgcttac ggatccgacc tgcaggcgga
     5641 catcgacgct gcggacttgc agaatctcga cggcgattcc agtttgtacc tccgtcttga
     5701 aaccttcgcg ctccactcgg accgagtaag ttcccgccgg aagaaaggag acacggtaaa
     5761 gcccgtcgcc ggaagttact gtagttctgg taagtccggt ttcttcatta cgaacggtta
     5821 ctttcgcatc cgtgatcact gcgtcggagg catcgcgtac ggtaccgctg atcgcaccgg
     5881 tagtggtttg gggcaaacag gggaccaccg cgagcacgga aaccgagacc acggccaaag
     5941 cgaaacgaag cggcaattga gacattccga agcacctctc gcgaaaagtt aaccgagtat
     6001 gtcataccgt cgacagtttt gcaataggga ttttggccag aggttttgca ggctcgcgtc
     6061 actccacccc agcaagcccg cccgccggaa ctttctccgc gcgcaaccgc cccaacaccg
     6121 ccgcaatagc gaatcccacc acaaaggaag ccggtaccag ccaggtgaac gaaatagact
     6181 gcccacgccc gaaggccacg tacaaggagc aggccgttcc cgccagaaag cctgccagga
     6241 cggaacgctt ggaggcaaag gggaataaga tgccggcgaa gaagagcacg gccagcggtc
     6301 cgacgaagat gtgattcagc cgctgcgtaa catcgatgag gttccagtcc gtacggcgaa
     6361 cggcccaggt gatggcagcg gcggcggcga ttccgatgag acctcccgca accgagacca
     6421 acttggtcag cgccagcgaa cttgcctccg tgcggtggcg gccaaagcgt tccaccaggt
     6481 ctttcatcac cacggccgcg agcgagttga ttccggagct gaggctcgac atggccgcgg
     6541 ccagaagcgc tgccagtacg agtccggaga cgccttgcgg cagctccttc acgatgaact
     6601 gcggcatcag ggcgtccgcg cggggagcga tctcgcgctg gaaatcggac accggcagac
     6661 cggactgttg aagatagaac ccgaacaagg ccaggccgca aaacgccagc accgccagca
     6721 gccccacgtt gatggcaatg tagacccagg cggtacggcg ggccatccgg atggagggag
     6781 tgctgagata gcgctgcact gccacctgat cgcttccatt cgtacagaca ctccagaaga
     6841 actgcgagag catgatgccg attacggtga ggcggacggt caagtcgagg ctcacgaact
     6901 ggatctgggt ccggccggcc tcggagaaag cgctccacca ttccaaaggc cccgtcccgg
     6961 tgcgcagta
//
LOCUS       DASTMK010000486         6842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_26329, whole genome shotgun sequence.
ACCESSION   DASTMK010000486 DASTMK010000000
VERSION     DASTMK010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26329"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFQ79_20355"
     CDS             <1..839
                     /locus_tag="VFQ79_20355"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU0142086.1"
                     /translation="VAIEHAKPVRIGVNWGSLDQSLLTRMMDENSKLPQPLDAAAVTR
                     NAIVASALLSAQNAESYGLAHNRIILSAKVSGVQDLIEVYRSLAAQCDYPLHLGLTEA
                     GLGSKGIVATTAALSVLLQEGIGDTIRASLTPLPNGDRTEEVIVCQQILQALELRSFT
                     PQVTACPGCGRTTSTFFQDMADQIQTYLRQQMPVWKEKYAGVEEMKVAVMGCIVNGPG
                     ESKHANIGISLPGTFEEPKAPVYVDGRLMTTLKGDAIVAEFIAILEEYVARRYSALEP
                     VA"
     gene            846..3857
                     /locus_tag="VFQ79_20360"
     CDS             846..3857
                     /locus_tag="VFQ79_20360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142087.1"
                     /translation="MRKSSSFLLVCLLVASQAFSKEIQATCGTHRDNLQEALRLHRDS
                     EQRRKRSAAARAEVQPQQISRVRDFGHIAVVEDSDGVVTRPNAFDLNRKTIVFHPSAP
                     DAPRYRFETTDVVFDAAALGDRLDLSDDDSVLRSIGFAFPFFGTAYREVYINSDGNLT
                     FGAGDESSSARSLGRVTAGPPRIAPLFRDLDPSRSSSGVRAFSDGTRFIVTWMEVPEY
                     SDFSNAPVQTFQVRLYPDGRIEFALQDITTKEALTGISPGMLQGGTDIVDFLNGASQE
                     YAGTIAESFGTDLEVDIVTTAQTFYSAHDDAYDYLVIYNTVRDERGEPVQAGDNAVAY
                     EVTVRNNRSGYGDIPMDVGQEFGSPYRLQSVLNLGPLSQYPNNPRSIVGARAISRDTP
                     MTVLAHEAGHLFLAYASVRDPLNPGARPMLGRQGAHWAFTFNSEASLLEGNRIEDRGA
                     TVDADSRFLTTKTVEGYSPLDQYLMGFRAAGEVSPTFLVENARNGTSSVSASRAPQPG
                     VAFGGRRREVTIQDIIQVEGRRTPDTTVSQRRFRFAFILIVPAGQDPSAEDLAKLERF
                     RAEFERFYSEATSQRAIADATLARSLRLSVFPAAGVLEGREVSGSVSIGSPATEDVTV
                     FLQAPNGFARVRSSVTIARGAQTASFSISGVRAGVEELTAETNSGFETVHVRLQVAPA
                     SALSLQIVSGNLQSGVPGVPLNRPVVFRVTDINNLPYPGVRVLATVSGGGVFTPDSAV
                     TDESGTVQFAWTPGQGAPNLLTTYLEGTAPEAGIVASVLGRPTLTTAGIVNSASFKPG
                     IAPGSLASIFGSNLSTTSPAGASLPLPARLGGTQVFLNGVPISLLYVSDRQINFLVPS
                     SASQGTAEIRVSTSAGDSPALALTLTPVEPGVFFSSSTGIGAVLVSGTGMSTAERPAT
                     RADTLEIYATGLGAVQRSTVYAGLEETVTRPEVSVGGVPAQVGFSGLAPGFIGLYQVN
                     AQIGAGTPSGLQPLTLTMNGVRSNEVPVVIQ"
     gene            complement(3859..>6842)
                     /locus_tag="VFQ79_20365"
     CDS             complement(3859..>6842)
                     /locus_tag="VFQ79_20365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142088.1"
                     /translation="GRELNTPQDVAVDAERDILYVADTGNNRVMAWRNASTVSGPAYA
                     DLIIGQRDRFSSLNLGPGSDLSTGLTSPTAVAVDREGNLYVIDAGNNRILRFPRPFEQ
                     TSDLKQPDLVLGQPNFNSRTANNGGVSERSLGFLIGESLYRSDLVFDPQGNLYVSDAG
                     NNRVLRYAAAELRSGNNNAAADLVLGQAFFNTRDAQANGTTGGVDNRVAAKDRMRSPS
                     GIALDDDGRLYVTDGVMPATSNPQTRVLVFEPPFVNGKAASAVMGVPVPAQGQTFVNQ
                     YNFFGAEGVFLIGNTPFVLDTNGSRLLRYPTYVNWTPDVSPGAVAVMGQSMNTPGPLF
                     PNAGSATASPTSFANPVRAAVAGGRVYIVDSGNNRILVFPNLAQGNSPAIRVIGQYEF
                     QYRAPNLLDERGMNFPAGVAVDRQSDPPRLYVADRNNHRVLGYSDARRLQPNAPADIV
                     LGQANFLSAVPNFPSGNADLPTETGLASPSAVAVDNDGNLWVADTGNGRVLRFPKPFD
                     RVGQQQRPDIVIGQAGFNLKIQDATRSTMSAPVGLAFTNEGHLLVSDAQHNRVLYFDK
                     DQLTNGAVAQKVFGQGDFFTSASGAERNRMNSPRHIAVDIDDRLYVADSGNNRVLLFD
                     RVPASPEANVDSAVSLENTTTTTRLRTPVGVAVYRDTGEIWVTDANNRLFRYPSFLQL
                     LTNPNAFAETSLAAAGPISASFDTFGNLLVGEGTNRVAIYYQASKLVNGANFGQARAN
                     DVGPGQRVVPGIIASIFRLGQPFTSQTVSFDSLPNPVPLPRVLADTQVLLNNEPVPLY
                     FVSPEQINFLVPMNAPESGEVQVQVVRQSTGQVLAVGCTAQFASNRYSCAGPLQADVA
                     SPGLFTRDASGTGQIAALNEDNTINSPENRISRGRVIQVFGTGQGRVPNAPPDGEFAQ
                     GETRTTGEIQVLIGTRFVDPSDIQYSGLAPGLIGVWQINVRIPEWVGISDVIDFVVVY
                     RSRLSNLQNGTVLKTTIAVRQ"
BASE COUNT         1348 a         2055 c         2050 g         1389 t
ORIGIN      
        1 aggttgccat cgagcacgcg aagcctgtgc gcattggtgt gaactggggt tcgctcgacc
       61 agtcgctgct cacgcggatg atggacgaaa actcgaagtt gccgcaaccg ctcgacgccg
      121 cggcggtgac ccgcaatgcc atcgtggcca gcgccctcct gtctgcccag aatgcggaga
      181 gctacggcct ggcgcacaac cgcatcatcc tgagcgcaaa agtcagcggc gtgcaggacc
      241 tgatcgaagt ttacaggtcg ctcgcggcgc aatgcgatta tccgctgcac ctgggcctga
      301 cggaagcggg gcttggatcg aaaggcatcg tcgccacaac cgccgcgctg tccgtgttgc
      361 tgcaggaggg cattggggac acaattcgcg cctcgctcac accgctgccg aacggagacc
      421 gcacggaaga agttatcgtt tgccagcaga ttctacaagc actggagttg cgcagcttca
      481 cccctcaggt gacggcgtgc ccgggttgcg gccggacgac cagcaccttc ttccaggaca
      541 tggcggacca gatccaaacc tatctccgcc agcagatgcc ggtatggaaa gagaagtatg
      601 ccggcgtaga ggagatgaaa gtagcggtga tgggttgcat cgtaaacgga ccgggcgaat
      661 cgaagcacgc caacatcggc atctcgctgc ccggaacctt cgaagagccc aaagcgccgg
      721 tttacgtgga tgggcggttg atgaccactc ttaaaggcga cgccattgtt gccgaattca
      781 tcgccatcct ggaagaatac gtggcgcgcc gctattcggc gctcgagccc gtagcgtagc
      841 aacatatgag gaaatcctcg tctttcctac tggtctgcct gctcgttgcc agccaggcat
      901 tctccaaaga aatccaggcc acttgcggaa ctcaccgcga taacctgcag gaagctctgc
      961 ggctgcaccg ggattcggag cagcggcgga agcgcagcgc cgcggcgcgc gcggaagtcc
     1021 aaccgcaaca gataagccgt gttcgcgact tcggacacat tgctgtcgtc gaggattcag
     1081 acggtgttgt gacgcgcccc aatgcattcg atttgaatcg caagacgatc gttttccatc
     1141 cgtccgcgcc ggacgcgccg cgttaccggt tcgaaaccac cgatgtcgtc ttcgacgcgg
     1201 ctgctctcgg agaccggctg gatctcagcg atgacgatag cgtgctgcga tccatcggtt
     1261 tcgcgtttcc gtttttcggt acggcgtacc gcgaagtgta tatcaattcc gacggcaatc
     1321 ttacgtttgg ggcaggcgat gagagcagca gtgcaaggtc gctaggccgg gtgaccgccg
     1381 gcccgccgcg tatcgcgccg ctgttccgcg acctcgaccc gagccggtcc tccagcggcg
     1441 ttcgcgcttt ttcggacggc acacggttca tcgttacctg gatggaggtg ccggagtatt
     1501 cggatttttc aaacgccccg gtgcaaacgt ttcaagtccg cctgtatccg gatggacgca
     1561 tcgaatttgc gctccaggat attaccacga aggaagcgct gaccggcatc tctcccggaa
     1621 tgttgcaggg tggaacggac atcgtcgatt ttttgaacgg cgcaagccag gaatacgccg
     1681 gtacgattgc ggagtcgttc ggtacagatc tggaagtgga catcgtcact acagcgcaga
     1741 cgttctactc cgctcatgat gacgcttatg actacctcgt gatctacaac accgtgcggg
     1801 atgaacgcgg cgagcccgtg caggcgggtg acaatgccgt tgcttatgaa gtgacggtgc
     1861 ggaacaaccg cagcggttat ggggacatcc cgatggacgt cgggcaggag ttcggatcgc
     1921 cctaccggct gcaaagcgtt ctcaatctgg ggccgttgag ccagtacccc aacaatcccc
     1981 ggagtattgt cggcgcccga gctatttcac gcgatacgcc catgaccgtg ttagcccatg
     2041 aagcgggcca tctcttcctc gcttatgcga gcgtgcgcga tccgctcaac ccgggtgcgc
     2101 gcccgatgct gggccgccag ggagcgcatt gggcattcac gtttaattcc gaagcctccc
     2161 tgctcgaagg gaaccggatc gaagaccgcg gtgcaacggt cgatgcagac agtagatttc
     2221 tcaccaccaa aacggtggag ggctactcac cgctcgacca gtatctgatg ggatttcgcg
     2281 cagcgggcga ggtttcgccg acgttcctcg tcgaaaatgc tcgcaatggg accagctcgg
     2341 tttccgcctc ccgcgcgccg cagccgggtg tcgctttcgg aggacggcgg cgggaggtga
     2401 cgattcagga cattatccaa gtggaaggcc gccggacacc cgatacgact gtatcccaaa
     2461 ggcgattccg tttcgctttc atcctcatcg tgccggccgg gcaggatccc agcgcggaag
     2521 acctggcaaa gcttgaaagg ttccgcgcgg agttcgagag attttactcg gaggcgactt
     2581 cacagcgtgc cattgcggat gccactcttg cgcgtagctt gcggctatct gttttcccgg
     2641 ccgccggtgt tctcgaaggc cgcgaagtat cgggttccgt ttccatcgga agccctgcta
     2701 ccgaagatgt gactgtcttt ctgcaggcac ccaacggctt tgcccgtgtt cgatcgtccg
     2761 tcacgattgc gcgaggcgcg cagacggctt cattttccat ttcgggtgtc cgcgcgggtg
     2821 tggaggagtt aacggcggaa accaactccg gctttgagac ggttcatgtg cgccttcagg
     2881 tggcgccggc ttcggccttg agtctccaaa ttgtctcggg caacctgcaa agcggagttc
     2941 cgggcgttcc cttgaaccgg cccgttgtgt ttcgtgtcac cgatatcaat aatctgcctt
     3001 atcccggcgt gcgcgtgctc gcaacagtat ccggcggagg tgtcttcaca ccggattcgg
     3061 ctgtaacaga tgaaagcggg accgtgcaat tcgcgtggac gccggggcag ggtgctccga
     3121 atctacttac aacctacctg gaaggcaccg cgcccgaagc cggtatcgtg gccagcgtcc
     3181 tggggcggcc cacgctcacc actgccggta ttgtgaattc ggcttcgttc aaaccgggta
     3241 ttgcaccagg ctcccttgct tccattttcg gatctaatct cagcactact tctcctgccg
     3301 gagcttcgct gccgctgccg gcaaggcttg gcggcacgca ggtattcctg aacggcgttc
     3361 ccatatcgct actctacgtc agcgaccgcc agattaattt tctggttcct tccagcgcat
     3421 cgcaaggcac ggccgagatc cgcgtgtcga cttccgccgg ggactcgccg gcgcttgctc
     3481 tcacgctcac tccggtcgaa cccggagtat ttttcagctc cagcacagga atcggtgcgg
     3541 ttctggtttc cggcacgggg atgagcacag ccgaacgccc ggccacacgc gcagacacgc
     3601 tcgagattta cgccactggc cttggtgccg tacagcgttc cacagtatat gcaggattgg
     3661 aagagacagt taccaggccc gaggtctccg tgggtggcgt gcccgcacaa gttggcttca
     3721 gcgggcttgc cccgggcttc atcggactct atcaggtgaa tgcgcagatt ggagccggta
     3781 ccccatcggg acttcagccc ctgaccctca cgatgaacgg ggtcaggagc aatgaagtcc
     3841 cggtggtgat tcagtagctt actgtcttac cgcgatcgtc gttttcaaaa cggtgccgtt
     3901 ttgcaggttg ctgagccggc ttcgataaac cacaacgaaa tcgattacat cggagatacc
     3961 gacccactcc ggaattcgca cattaatctg ccatacgccg atcagacccg gagccaggcc
     4021 cgagtactgg atgtcggacg ggtcgacgaa gcgagttccg atcagaacct gaatctcgcc
     4081 tgtggtccgg gtttctccct gtgcaaattc accatcgggc ggtgcgttcg gaactcgtcc
     4141 ctgcccggtg ccgaacacct gaatcacccg gccgcgggaa atccggtttt caggcgaatt
     4201 gatggtgttg tcttcgttca atgcggcgat ctgcccggta ccgctcgcgt ccctcgtgaa
     4261 cagcccgggg gaagcgacat cggcctggag cgggcctgca caggaatacc ggttcgaagc
     4321 aaattgagcc gtgcatccca ctgccagcac ctgtccggtg gactgccgca cgacctgtac
     4381 ctgtacctct cccgattcgg gagcgttcat cggcaccagg aagttgatct gctccgggga
     4441 gacgaagtac agcgggaccg gttcgttgtt cagcaacacc tgggtgtcgg ccagcactct
     4501 cggtagagga accgggttgg gtagcgagtc gaaagaaacg gtctgggaag tgaagggttg
     4561 gcccaggcgg aaaatggacg caattatacc tggaactaca cgctgtccgg gaccgacgtc
     4621 gttcgcgcgc gcctgtccga aattcgcgcc gttcaccagc ttagatgcct ggtagtagat
     4681 agcgacgcgg tttgttcctt cgcccaccag caggtttccg aaggtgtcaa acgacgctga
     4741 aatgggcccc gcggcggcaa gcgacgtctc ggcaaaggcg ttcgggttgg tcagcaattg
     4801 cagaaatgac gggtaccgga acaaacggtt gttcgcatcg gtcacccaga tttcaccggt
     4861 atcgcggtac accgccaccc cgaccggagt ccgcagacgg gtcgtcgtcg tggtgttctc
     4921 aagcgacacg gcagagtcga cgttggcttc cggcgacgcg ggcacgcggt caaacagcag
     4981 cacgcggtta ttgccgctgt cggcgacata caacctgtca tcgatgtcca cggcgatgtg
     5041 tcgcgggctg ttcattcgat ttctctcggc cccgctggcg gaggtgaaga aatcaccctg
     5101 tccgaaaacc ttctgtgcca ccgcaccatt cgtcaactgg tctttgtcaa agtacagcac
     5161 gcggttgtgc tgggcatcgg agaccagcag gtgtccctcg ttggtgaagg ccaacccgac
     5221 gggtgcgctc atggtggagc gggtcgcatc ctgaatcttc aaattgaacc cggcctggcc
     5281 aatcacgatg tccggtcttt gttgctgacc caccctgtcg aaaggcttcg ggaaccgcaa
     5341 cacgcggccg ttgccggtat ccgctaccca aagatttcca tcattatcca cggctaccgc
     5401 cgacggtgat gccagacctg tttccgtcgg tagatccgca ttcccggaag gaaagttcgg
     5461 aaccgcactc agaaaattgg cttggccgag cacgatatcg gcgggggcat tgggctggag
     5521 gcggcgcgcg tccgaatatc ccaggacgcg atggttgttc cggtccgcga catataaccg
     5581 gggcgggtcg gactgccggt ccacggcgac gcccgctggg aaattcatcc cgcgctcgtc
     5641 caggagattc ggtgcgcgat actgaaattc gtactggcca atcacgcgaa ttgcgggaga
     5701 attcccctgg gcgagattgg ggaacacgag aatccgatta ttgccggaat ccactatgta
     5761 aaccctgccg cctgcaaccg cggcgcgcac cggattcgca aagctggtag gggaagctgt
     5821 cgccgacccg gcgttgggaa acagggggcc tggagtgttc atagactggc ccatcaccgc
     5881 aaccgcgcca ggtgagacat cgggcgtcca attcacataa gtcggataac gcagaagccg
     5941 cgaaccgttg gtgtccagca cgaagggcgt gttgccgatc agaaagactc cctcggcgcc
     6001 gaagaagttg tactggttca cgaaagtttg cccttgcgcc ggtaccggaa cacccatcac
     6061 cgcgctggcg gccttgccgt ttacaaacgg cggctcgaat acaagaacgc gagtctgcgg
     6121 attgctcgtc gcgggcatta cgccgtcggt cacatacaaa cgcccgtcat catcgagggc
     6181 aattccggaa ggcgaacgca ttctgtcttt cgcggccaca cggttgtcca caccgcctgt
     6241 cgtcccattc gcctgcgcgt cgcgcgtatt gaaaaaggct tggccgagca cgagatcggc
     6301 tgcggcgtta ttgttaccgg accgtaactc cgcagccgcg tagcgcagca cccggttgtt
     6361 gcccgcgtcg gatacataca agttcccttg cgggtcgaag accaagtcgc tacggtacag
     6421 gctttcccca atcaggaatc cgagtgacct ctccgacacg ccgccgttat tggcggtcct
     6481 gctgttgaag tttggctgcc caaggaccag gtcgggttgc ttcagatccg aagtctgttc
     6541 gaacggccgg ggaaatcgaa ggatacgatt gttgccggca tcaattacgt agagattgcc
     6601 ttcacggtcc actgccaccg cagtgggtga agtcaatccg gtgctcaggt cggagcccgg
     6661 gccaagattc aaactcgaaa agcggtcgcg ctgccctatg atcagatccg cgtaagccgg
     6721 acccgaaacg gttgaggcat tcctccatgc catcacccgg ttgttccccg tatccgcgac
     6781 atacagaata tctcgctccg catccaccgc gacgtcctgt ggtgtgttga gttcgcgtcc
     6841 ct
//
LOCUS       DASTMK010000487        16053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_344605, whole genome shotgun sequence.
ACCESSION   DASTMK010000487 DASTMK010000000
VERSION     DASTMK010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16053
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344605"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /gene="cas7u"
                     /locus_tag="VFQ79_20370"
     CDS             complement(<1..498)
                     /gene="cas7u"
                     /locus_tag="VFQ79_20370"
                     /inference="COORDINATES: protein motif:HMM:NF021156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HEU0142089.1"
                     /translation="MADIKIEDLLKQDGPVAIIIKQPLTPVDEDERIIFPPTYPMTKW
                     NGRVHTIRDGDYRVTVELPTGSKTDKDEKRQNQKPGYQIDYFPDGTNICEIDSPQSQS
                     NRIEPKFKTIKDGKLVPQVEIHVGKDTVNLLDAGHRAGDAVVRMSSLAAEIHDAFTAA
                     KSGDFF"
     gene            complement(491..1309)
                     /locus_tag="VFQ79_20375"
     CDS             complement(491..1309)
                     /locus_tag="VFQ79_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142090.1"
                     /translation="MTGDAEPSITVNVDVTNPGQFFACCGLLELADRLWSRDEVAGGF
                     GPCYFGRSQFKIFGKTRFTTQDLMRALLACRRSPVDPYQPIMGSNAKPVKDARKTQPI
                     QLDGRIILRLAWWLDELAGRQTPFKMWGAHQTSEGLINNMAAAVNVDDVDDTTILESR
                     TGMTGRIGLDTRSSWNTLDEGFSPNDQNLPVDTYPATELLAAIGLQTFSPLRHNDGYI
                     YACWNHPLPTPVARAVASGTVMIRGVTRYRFEIEARGKFKYFTKASLLEGNTNG"
     gene            complement(1306..3909)
                     /gene="cas3u"
                     /locus_tag="VFQ79_20380"
     CDS             complement(1306..3909)
                     /gene="cas3u"
                     /locus_tag="VFQ79_20380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HEU0142091.1"
                     /translation="MASGTLDFAGRFYALTGNKPFPWQTALYDRFIAEDVPRSCDLPT
                     GVGKTSVIAVWLIALAAGARVPRRLVYIVNRRTVVDQTTDEAIKLRDNAAKSGIGHLA
                     ISTLRGRFADNQEWSVDPSRPAIICGTVDMIGSRLLFSGYRIGFKSRPLHAGFLGQDA
                     LLVHDEAHLEPAFQKLIEAIESEQNRCKDLNKVRIMALSATGRDYGGNGRDDGNKLFG
                     LTEEEKQPPEVIPDPPTEPIHHLWRRLKAEKSLRLHEAKSEVVAERVAELASAHEGSG
                     AAVLIYVMSLDDVATIERELKRTKRRIVLLAGTMRGKERDDLVEKAEFRRFLKGAEPG
                     ETVYLICTSAGEVGIDISASHMICDLSSFESMAQRLGRVHRYGEPIGHVARIDVVHPA
                     SFGKIDKKTGELKADELDIRRSKTLEILRQLPSMEDGVHDASPKALGDLRERSDLPYK
                     IEDAFAPEPTILPATDILFDAWALTSIRQRMPGRQPVEPYLHGIAAWQPPETHVAWRQ
                     EVEIITGGLLDRYPPEDLLENYPLKPRELLRDRSDRVFTQLALLAKRHPEKPVWAIDD
                     RGAVETTTLEQLADKEWKDRIEGCTVLLPPSAGGLNGGLLDGASEEADDVADITSASA
                     TSRIRVWSDDPEYESKTADLRRVRSIEVSTFEDDGAEPRFWEWFESFPIEGGSTAKGK
                     VRWETHVSDVVCRTKQIVAGLTLPPGVADAVILAAKLHDHGKRWERFQITLGNLDYPN
                     VLLAKSGPRGARLSEPFRHEFASVLDAQADPELNRLTGELKDMVLHLIAAHHGRARPH
                     FGIDEAFDPERPFCETQALADETPRRFARLQRKYGRWGLAYLESLLRAADWAASAEPS
                     EFVTGTKEVLR"
     gene            complement(4210..6684)
                     /locus_tag="VFQ79_20385"
     CDS             complement(4210..6684)
                     /locus_tag="VFQ79_20385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0142092.1"
                     /translation="MTEHNAAGFLRAVLWTVFAGTAAVFAYGLATERLFAQEIWTRTG
                     LERLLAVSAGYVALAAFCFPWRQFFGPALAALGLCYAGVAAGPTAVAASLLFVLSCYA
                     LGDLLRSKPHHAGSFDAALSFLTGAAVYIGLIGIAVHFPVNRPLFYFVLLGGPLFLNR
                     PRVVAVLRHFLTPSNNPSRPDEYLLMSLTGFIAVLHLLAVLKPEVSADGLAMHLAVPA
                     WVARHGYWNFDVTRFTWGVMPMGGEWCYTAGYLLGGEYAARLINFAMLLAGVAVLFEL
                     LRRYLPRRDAFLCVALFASTPLVQLVTGSLFVENVWMALLLAAFACTLRFRETWQASH
                     LYLAALLAGAALATKLLAAPYILLMSIFLFHDLWVGIRSGKVTRPARLLLVAVACLVV
                     IGAQPYATAFIKSGNPVFPLLNAIFHSPYFDQTGESSDMRFREPLQWNTLYKLTFQTS
                     KYLEGQAGAFGFQWLVFLPLAIVMLGARWPYAGWVALATSLAYTVAVFQGQGNVRYLY
                     PVMPLLSLAVGLVFARVQPGGPPLYRLLSFTAIVVVLLNIGFLPASGWYHKDLALWPL
                     TGPARAAYIEGIAPVRTLVQFLNTHFPGQPVAFIETSDIAELDAESYAASWHDYLFAR
                     QLAGAHSAEDIHRVLKQAGIGLIISPLPGSSVPVPQVAMEEFLSRYVDPVAMAAGYQA
                     ARVRAMPLPRQTGTIVSAGDHDDAAGDIAYHGAWSHNHNFPETRNRTVTYSNVPGDRF
                     TLRFQGTEIVWVHTRAANRGIAEIFLDGTKIAELDLYRNATEWQAEARYAAPGPGPHE
                     FEVRVSGRKNPASTGVFVDVDALTVR"
     gene            complement(6681..7100)
                     /locus_tag="VFQ79_20390"
     CDS             complement(6681..7100)
                     /locus_tag="VFQ79_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142093.1"
                     /translation="MLQKQIISWLIGFFQTERGQAILSDLLAKAAKGWKPGTRPEPQS
                     SPSATPENTGEAIPDPLDELIPRLQAVEAAAVRLEGGVQNSLSGVREINEGMARTNLQ
                     IARLERQTRVLEKRLWWALVGNAATIAAVIALALTRA"
     gene            7216..8964
                     /gene="dnaG"
                     /locus_tag="VFQ79_20395"
     CDS             7216..8964
                     /gene="dnaG"
                     /locus_tag="VFQ79_20395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU0142094.1"
                     /translation="MDLAKHLKSSIDIVKVIGEYVRLRRTGAAGRYMGLCPFHTEKTP
                     SFSVNGTHQYYYCFGCNAKGSAIDFVMQLDGLTFFEAVKSLAERFGVTLPRRAEYSDE
                     QTKLRAALYQMHELAARIYRSNLNSPAGSDTRDYLARRGLSPATIEEFGLGLSGNSWQ
                     TLARRFEQEGFSREQMEASGLVLQRQDGSGFFDRFRGRLMFPIHDESGRIIGFGGRAM
                     AAGDESKYLNSSETLLYRKRHVLYNLHRAKTSIRKQERVILVEGYMDVIGVAAAGIGE
                     VVAGCGTSLKDTQVRIIHRHCDRVVVNFDPDTAGANAAERSIDILLGEKLNIRILELE
                     EGLDPDEYIQRFGANRYREKADKAAGYFHWLADRARTRHDDGTAQGKWAAFQAVLPAI
                     VKNSDKLEQAAVANDIASYLGVDANLVRDRLRSAGYENRGRAEKRAESNLPPVERILL
                     NLLLAGGGVREKIIPELVALPGMEQLQARRLFETVCALYTQQGDFTFEDVEARLDDAG
                     KALLTSVIFADEVGGESGSVEQARACLRELRLGCRKTAESALRSKIRAAEKVGDITEA
                     LRLTEELSRLQREVNV"
     gene            9039..10709
                     /gene="rpoD"
                     /locus_tag="VFQ79_20400"
     CDS             9039..10709
                     /gene="rpoD"
                     /locus_tag="VFQ79_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU0142095.1"
                     /translation="MAGEEKYGRLKNLVDLGKEKGFVLYDDVSEFLPDDIAEGPELDD
                     ILTDLDSAGIEILDEPKKNIDGDDYSDADLSQEFGEKTNDPVRMYLREMGTVPLLTRD
                     GEIELARRIEHGQKAVQKALSRSPLIIREILHLGSTLNPEDGSIRDILSLPDLLLSDE
                     AAVEHALRFLAALAEIETHFRRAQQYRLRMQSASSSLRPKQIRGLRFSLARELVMVSQ
                     MVRDLQLSPIIRRRLAGCLRRTVEEFRDLERELARAQRRSEEAANASASPQREGKREL
                     RQLAARLEQLEQERGASSTELRRTLQIVERGEAEAELAKKQLIEANLRLVVSIAKRYT
                     NRGLQFLDLIQEGNIGLMKAVDKFDYRRGYKFSTYATWWVRQAITRAIADQARTIRIP
                     VHMIETINKLVRTQRMLQQKLGRDPTTEELGRELELPVGKVRKVLRIAQEPISLETPV
                     GEEEESHLGDFIVDRRVTSPSEAVINLNLREQTAEVLKTLSPREEKIIKMRFGLQDGS
                     EHTLEEVGQHFAVTRERIRQIEAKALRKLRHPSRSHRLRAFLDTSCHE"
     gene            complement(10700..11299)
                     /locus_tag="VFQ79_20405"
     CDS             complement(10700..11299)
                     /locus_tag="VFQ79_20405"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142096.1"
                     /translation="MRFFRRACASMTRLAIFPGTFNPPTIAHEAMAMAAIPLVDEVLY
                     VLPEAFPHKEYDTGAGFEDRIHLLLRMAEGTPCSVASTKGGLFIDIARECRMAYGAAT
                     QLYFLCGRDAAERIVMWDYGEPGAFARMLGEFELLVAGREGVYEAPAEFRSRIHNLNL
                     DRDYSGCSASQVRASIARKEPWEHLVPAAIRDEVRRIYS"
     gene            11358..12026
                     /locus_tag="VFQ79_20410"
     CDS             11358..12026
                     /locus_tag="VFQ79_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142097.1"
                     /translation="MFTWICQKCGAEVPPSYSECPNCAPKPEGQKEPEPVPGAPPPSL
                     AAAPPPQEKEKKAKPAQRPGSKPALPGWLVALIVAGVITGIGALAYFYFLPAVRTRSQ
                     AATPATRLESVPATSGSPIAKHLELTGIRITEDTRKRVEVRLIVVNHSAAEIPDLELS
                     VDLKPSNASPETKPVASFDMKVPGLGPFEAKEVVTTAKTELRAYEIPDWQFLAVNFRV
                     MSPK"
     gene            12040..12717
                     /gene="nth"
                     /locus_tag="VFQ79_20415"
     CDS             12040..12717
                     /gene="nth"
                     /locus_tag="VFQ79_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU0142098.1"
                     /translation="MLTPVRPVKPKTRAERRLRVEAILSKLDEMYPAATCALVHHTPW
                     ELLVATILSAQCTDKRVNEVTPELFRKYPTLEDFAAVSQAELARDIRSTGFFNNKAKS
                     LIGAARKIITDFGGEVPRTMDEMLTIPGAARKTANVVLGTAFGIASGIVVDTHVQRVS
                     RRLDLTRNTEPVKIEQDLVKTIPKEKWILFSHQIIHHGRALCIARKPKCAECGLYSLC
                     YSKDKTA"
     gene            complement(12751..13467)
                     /locus_tag="VFQ79_20420"
     CDS             complement(12751..13467)
                     /locus_tag="VFQ79_20420"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0142099.1"
                     /translation="MKLLCAVLLLSLPIAGQKPEFDFYRDARDKKPEVYAAELRTAGV
                     PEKEIVRRLDLLKNHRHLLEADRWNRFYSDPKNNYNRAPNSFLTVVVEDMKPGTALDY
                     AMGDGRNAIYLARLGWTVHGFDMSEVAIDIAKHRAAEAGLKIDAVVSTDANYNFGKER
                     FDLILFSWSMPLVDVHKVIDALKPGGIVVMEFGPGFVGRNGMLRLFDGLLIERYEMTH
                     AVSDFSDRRKTEIFRLIARK"
     gene            complement(13527..13880)
                     /locus_tag="VFQ79_20425"
     CDS             complement(13527..13880)
                     /locus_tag="VFQ79_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0142100.1"
                     /translation="MVLVDTSVWIAHLRKRQPVLADLLWNGLVVMHPFVTGEMACGNL
                     RKRAAILADLAALPLATHASDAEVMRLVEDRRLWGRGLGWIDAHLLASALISHCRLWT
                     LDKRLRVAAAELGVS"
     gene            complement(13880..14071)
                     /locus_tag="VFQ79_20430"
     CDS             complement(13880..14071)
                     /locus_tag="VFQ79_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEU0142101.1"
                     /translation="MRTTIIIDDALLERAREATGIQEKTALVRAGLEALIAREAGKRL
                     AALGGTQPGISGIPRRRSA"
     gene            complement(14160..14636)
                     /locus_tag="VFQ79_20435"
     CDS             complement(14160..14636)
                     /locus_tag="VFQ79_20435"
                     /inference="COORDINATES: protein motif:HMM:NF042490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6650 family protein"
                     /protein_id="HEU0142102.1"
                     /translation="MKWNEAANRLNGFSIASIGVQWTPKPTDSEVARRVIQFLEDRRV
                     LYSDYAWEEPNHCIESVLEIRRFLTSEIADLTHGSDLLSPFRAIRAACRKFLDRMHHD
                     GHRRVRLHHGMESFDFYSALGELRGCIGPQIALLAASYKVDVEDELARILPVPDQE"
     gene            15087..15644
                     /locus_tag="VFQ79_20440"
     CDS             15087..15644
                     /locus_tag="VFQ79_20440"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0142103.1"
                     /translation="MREDKNKRWQTALKLLLVAVIVGLISFGLDLGSAVDTHTYAPGV
                     RKPMPHFEYRDLSGATWNLDEQRGKALLVNFWATWCPPCRRETPDLVKLSREYSQEEF
                     VIIGVSLDDGAEQVRRFVSQFDVPYPVVMPRENDPLSAQIQSLPTSILVDPEGRIARM
                     YVGAVSASQLRKDLQQLFGEFRPTS"
     gene            complement(15634..>16053)
                     /locus_tag="VFQ79_20445"
     CDS             complement(15634..>16053)
                     /locus_tag="VFQ79_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0142104.1"
                     /translation="RDGLLPKIFSDIHPKWSTPYRSNMLFMVFVSLFSAFAPISVVGH
                     MTSIGTLFAFSLVCAGILVMRRTHPDLARPFRTPLVPLVPLLGILVNLALMYGLGWEN
                     WLRLFVWLVIGLVIYFTYSRYHSNLARHPQTDKLSRK"
BASE COUNT         3392 a         4746 c         4754 g         3161 t
ORIGIN      
        1 gaagaaatcg ccagatttgg cagccgtgaa agcgtcgtga atctcagcag ccaatgagga
       61 catgcggacc acagcgtcac ccgctcggtg tccggcgtcg agcagattta cggtatcctt
      121 acccacgtga atctcgacct gagggacgag tttcccatct ttgatcgtct taaatttggg
      181 ttcaatgcga ttgctctgcg attgtgggct gtcgatctcg cagatattgg tgccgtccgg
      241 gaaatagtca atctgatagc ccggcttttg attctgcctc ttctcgtcct tgtccgtctt
      301 ggaaccagtt ggcaactcca ccgtcacgcg atagtcgcca tctcgtatcg tgtggacccg
      361 gccgttccac ttggtcatcg gataggtcgg cggaaagatg atgcgttcgt cctcgtccac
      421 cggtgtaaga ggctgcttga tgatgatggc aaccggtcca tcttgcttaa gcaaatcttc
      481 aatcttgatg tcagccattg gtattccctt caagcaatga tgccttggta aagtatttga
      541 acttacctct tgcttcgatc tcgaatctgt agcgggtaac gccgcgaatc atgaccgttc
      601 cagaagcgac ggctcttgct actggcgtcg gcagtggatg gttccagcat gcatagatat
      661 agccgtcatt gtgtcgcagg gggctgaatg tctggaggcc gatagctgcc agtagttcag
      721 tcgccggata ggtatcgaca ggaaggtttt ggtcatttgg ggaaaatccc tcgtcaaggg
      781 tgttccaact gcttctcgta tctaggccga ttcgcccggt catgccggtc cttgactcta
      841 ggatggtagt atcgtccaca tcatctacat tgaccgcagc agccatgttg tttataagcc
      901 cctcggacgt ctgatgggct ccccacatct tgaacggcgt ctggcgccct gcgagttcat
      961 cgagccacca agcgagccgt aagataatac gaccatccag ttgaatcggc tgcgtctttc
     1021 gtgcgtcctt gaccggctta gcgttcgacc ccatgatagg ttgatacgga tccactggag
     1081 atcgtctgca ggccaggagg gcacgcatta aatcttgcgt cgtaaatctg gtcttgccga
     1141 aaattttgaa ttgcgatctg ccgaagtaac atggaccgaa cccacccgca acctcgtcac
     1201 gagaccacag acgatccgcc agttccaaca ggccgcaaca ggcgaagaac tgaccggggt
     1261 tggttacgtc gacattcaca gtgatcgatg gctccgcgtc gccggtcatc gcaacacctc
     1321 cttggtcccg gtcacaaact cagatggttc ggcgctagca gcccaatcgg ccgcgcggag
     1381 caaggattcg agatatgcca ggccccatcg gccgtacttg cgctgaagac gggcgaacct
     1441 gcgaggcgtc tcgtcagcca gcgcctgggt ctcgcagaag ggccgctccg ggtcaaatgc
     1501 ttcgtcgatc ccaaaatgag ggcgagcgcg gccgtggtga gcagcgatca gatgcagcac
     1561 catgtccttc aactccccgg taagtctgtt caactccggg tcggcctgag catctagtac
     1621 cgaagcgaac tcgtggcgaa acggctcaga cagtcgcgcg ccacgtgggc ctgacttggc
     1681 aaggagaaca ttcgggtagt ccaggtttcc cagagtgatc tggaatcgtt cccatctctt
     1741 gccgtggtcg tgcaactttg ccgcgagaat gactgcgtcc gcgacgcctg gaggaagcgt
     1801 caggcccgcc acaatctgtt tcgtgcggca caccacatca ctgacatgcg tctcccacct
     1861 cactttcccc ttggccgtac ttccgccctc aatgggaaaa ctctcgaacc attcccaaaa
     1921 gcgtggttca gctccgtcgt cctcgaacgt gctgacttct atgctgcgca cgcgacgcag
     1981 gtcagcggtc ttgctctcgt attccggatc atccgaccag acacggattc gcgacgtggc
     2041 ggaggccgat gttatatcgg ccacgtcgtc agcctcttcg gacgcaccgt cgagtagccc
     2101 accattcaaa ccgcccgcgc tagggggcag aaggaccgtg caaccctcga tgcggtcctt
     2161 ccactccttg tccgccagtt gctccagtgt cgtcgtttcg acggctccgc gatcatcgat
     2221 cgcccatacg ggtttctcag ggtgtcgttt cgccaggagc gctagttgtg tgaaaacccg
     2281 gtcgctgcga tcgcgcagca gttcgcgcgg cttgagcggg tagttctcaa gcaaatcctc
     2341 gggcggatat cgatcgagca gacccccggt aatgatctcg acctcctgcc gccacgcaac
     2401 gtgcgtttcg ggcggttgcc acgcggcgat gccgtgcagg taaggctcca ccggttgccg
     2461 gccgggcatt cgttgccgga tgctggtcaa tgcccacgcg tcgaaaagga tgtcggtggc
     2521 tggaaggatc gtcggctccg gcgcgaaggc atcttcgatc ttgtagggca ggtccgagcg
     2581 ctcacgcagg tcgcccagag ccttgggact ggcgtcgtgc acgccgtctt ccatagaggg
     2641 cagttgcctg aggatctcta gcgtcttgct gcggcggatg tcgagttcat cagccttcag
     2701 ttcgcccgtc ttcttatcga tcttgccgaa cgatgccgga tgcacgacgt cgatacgtgc
     2761 cacatgaccg atcggctcac cgtaacgatg taccctaccc aggcgttggg ccatgctctc
     2821 aaacgaagat aggtcgcaga tcatgtggct ggcggagatg tcgatgccca cttcgcccgc
     2881 gctggtgcag atgaggtaca cagtctcgcc cggttccgct cctttgagaa atcgcctgaa
     2941 ttcggccttc tccacgagat catcacgctc tttgccccgc atggttcctg cgagcagcac
     3001 gatccgccgc ttggtcctct tcaactccct ctcaatagtc gccacgtcat cgagcgacat
     3061 gacatagatg agcacagccg cgccgctgcc ttcgtgcgcg gaggccagtt ccgctacccg
     3121 ctcggcaacg acttcactct tggcttcgtg cagccgaagt gacttctccg ctttcaatcg
     3181 ccgccagaga tggtggatcg gctccgtggg tggatctggt atcacctccg gcggctgttt
     3241 ctcctcttcc gtcagcccaa acagcttgtt tccgtcatcc cgcccgtttc cgccgtagtc
     3301 cctccccgtc gccgacaacg ccataatccg gaccttgtta agatccttgc accggttctg
     3361 ttcggattcg attgcctcga tcagcttttg aaacgctggc tccaggtggg cttcgtcatg
     3421 cacgagcaaa gcgtcctgtc cgaggaaccc ggcgtgcagc ggccggctct tgaacccgat
     3481 gcgatagcca ctgaacagca agcgcgagcc gatcatgtcc accgtgccgc atatgattgc
     3541 gggccgggat ggatcaaccg accattcctg attgtcagcg aaccggccgc gcaacgtgct
     3601 gatggcaaga tggccgatac ccgattttgc cgcattgtct cgtaacttga ttgcttcatc
     3661 ggtggtctga tccaccaccg tgcgcctgtt gacgatatac accaatcggc ggggcactct
     3721 cgcgccggca gctagcgcaa tcagccatac cgcaataacg ctcgtcttgc ccacgccagt
     3781 tggcaagtcg catgaccggg gcacgtcctc cgcgatgaac cggtcgtaca gcgcagtctg
     3841 ccatggaaac ggtttgtttc ccgtcaacgc atagaatctt cccgcgaaat cgagcgtgcc
     3901 gctagccatt gctccgttgt ccctaaagcg aaactgtagt atatagcagc ttaaacgatt
     3961 ctaaacactt aataagctcg aaaagactca atcccgtctg ctcaggcccc attggcgccc
     4021 gacttcagac agatttcctc ggcaacttcg atgccttatc gtcaccctac cgtgtattct
     4081 gatgttctgt gctgcactca gccttggaag agaatcgcca gcctggaatt tgaattgccg
     4141 ttcggcgatt gaaaaccagt tctaattagt tccaggcgca agggctgcgc gcccattcac
     4201 gaaggcggtt catcgcaccg tcaaagcgtc cacgtcgaca aacacacccg tagaggcggg
     4261 gttcttgcgg cctgagaccc gcacttcgaa ctcgtgcggg ccggggccgg gggcggcgta
     4321 acgcgcttcg gcctgccact cggtagcgtt gcgatagaga tccaactccg cgatctttgt
     4381 gccgtccagg aaaatctcgg cgatgccgcg attcgccgcg cgggtgtgga cccagacgat
     4441 ctccgtgccc tggaaacgca gtgtgaaccg gtctccggga acattggagt aggtcacggt
     4501 acgattccgt gtctcaggaa aattgtgatt gtgcgaccat gcaccgtgat acgcaatgtc
     4561 accagcggca tcgtcgtggt cgcccgccga aacaatggtc ccggtctgac gcggcagggg
     4621 catcgcccgc acacgcgccg cctgatatcc ggcggccatg gctaccgggt ccacatagcg
     4681 gctcagaaat tcttccatcg ccacctgagg gactgggacg gagctgcccg gaagagggga
     4741 aattatgagg ccgattccgg cctgtttcag cacgcgatgg atgtcctctg ccgagtgggc
     4801 gcctgccaat tgacgtgcga aaagatagtc gtgccaggat gcggcatacg attctgcatc
     4861 cagctccgcg atatccgagg tctcgatgaa agccaccggt tgccctggaa agtgcgtgtt
     4921 cagaaactgt accaatgtgc ggaccggagc gattccttca atgtaggccg cgcgtgccgg
     4981 accggtgagc ggccacagag cgagatcttt gtgataccaa cccgaagccg gcagaaagcc
     5041 gatgttcagc aacaccacca caattgccgt gaaagacagc aaccggtaca gcggtggtcc
     5101 gcctggctgc acgcgggcga acaccaggcc tacggcgagg ctgagcagcg gcattaccgg
     5161 gtagagatat cgcacgttcc cctgcccctg aaaaaccgca accgtgtagg ccaatgaagt
     5221 agccagggcg acccatccgg catagggcca gcgcgcgccc agcatcacga tcgcgagcgg
     5281 cagaaatacc agccactgaa atccgaacgc tcccgcctgc ccttccagat atttcgaggt
     5341 ctggaaagtc agtttgtaga gggtgttcca ttgcaagggt tcgcggaaac gcatgtcgct
     5401 cgactcaccc gtttgatcga aatacggtga gtggaagatg gcgttcaaca gaggaaacac
     5461 gggatttccg cttttgataa acgccgtagc gtaaggctgg gcgcctatca cgaccaggca
     5521 ggccaccgcg acgagtagga gccgggccgg gcgcgtgacc ttgccggaac ggattcccac
     5581 ccataggtca tgaaaaagga agatcgacat cagcagaata taaggggcgg caagcagctt
     5641 cgtcgccagt gccgcgcccg ccagcagcgc ggcaaggtag agatgcgaag cctgccaggt
     5701 ctcccggaag cgcaacgtgc aggcgaacgc cgccaacagc aacgccatcc agacgttttc
     5761 gacgaaaaga gacccggtca ccaactgcac caggggtgtg gaggcaaaca gcgccacgca
     5821 cagaaacgca tcgcggcggg gcaggtaccg ccgcagcaac tcgaaaagta cggcaacgcc
     5881 cgccagaagc atggcgaaat tgatcaggcg cgcggcatac tctccgccga gcagataacc
     5941 tgccgtatag caccactcgc ctcccattgg catgacgccc caggtgaaac gcgtcacgtc
     6001 gaagttccaa tagccgtgcc gggccaccca agccggaacc gcgaggtgca tcgccagtcc
     6061 gtcggcgctc acctccggtt tcaggacggc gaggagatga agtacggcga tgaaaccggt
     6121 gaggctcatc agcaggtatt cgtccggacg cgatggattg tttgacggag tcaggaaatg
     6181 gcggagtacc gcgaccactc tcggcctgtt cagaaaaaga ggtccgccga gcagcacgaa
     6241 atagaacagc ggccggttga cgggaaaatg gacggcgatg ccgatgagcc cgatatatac
     6301 ggccgcgccg gtgagaaacg acagcgcggc atcgaagctg ccggcgtgat gcggcttcga
     6361 gcggagcagg tctccaagcg cgtagcagga aagaacgaaa agaaggcttg ccgctacggc
     6421 ggtgggtccc gcggccacgc cggcatagca gagccccaga gcggccagag ccggcccaaa
     6481 gaattgccgc cagggaaaac aaaacgcggc cagcgcgaca tatccggccg acaccgccag
     6541 cagacgttcc agacctgtgc gggtccagat ttcctgggcg aacagccgct ccgtagccag
     6601 tccgtacgcg aatacggcgg cagttcccgc aaacaccgtc cagagaaccg cgcggaggaa
     6661 gccggcagcg ttgtgttccg tcatgccctg gtcagcgcca gcgcgatcac ggccgcgatg
     6721 gtggccgcgt taccaaccag ggcccaccat agacgtttct cgagcacgcg ggtctgccgc
     6781 tccagacggg caatctgaag atttgtgcgg gccatcccct cattgatttc gcggaccccc
     6841 gagaggctgt tctgtacgcc gccctccagt cggactgccg ccgcttccac cgcttgaagc
     6901 cgcggaatca attcatccag cgggtccggt atcgcctcgc ccgtgttctc cggtgtcgcg
     6961 gagggggaac tttgcggttc cgggcgtgtg cccggcttcc agccttttgc ggctttcgcc
     7021 agcaggtcgg aaaggatggc ctggccccgc tccgtctgaa aaaatccgat cagccaggag
     7081 atgatctgct tctggagcat aagacgttag cgtatgactg cgccgcgcgc ctcggcaagc
     7141 gccggggggc cctcggcgaa tgccggtggg gctaagatag gaagttgacc ggcacacccg
     7201 ccgtctcgct gatcgatgga tctcgcaaag catctgaagt cttcgatcga tatcgtcaaa
     7261 gtaattggcg agtatgtgcg cctgcgccgg accggcgccg cgggacgcta tatggggctg
     7321 tgcccgtttc acaccgagaa gacgccgtcg ttcagcgtca acgggacgca ccaatactac
     7381 tactgcttcg ggtgtaacgc caagggaagc gccatcgatt tcgtgatgca gttggacggc
     7441 ctgacgtttt ttgaggccgt caagtcccta gcggaacgtt tcggtgtcac gttgccgcgc
     7501 cgggccgagt actccgacga gcagaccaag ctccgggccg cgctctatca gatgcacgag
     7561 ttggccgcgc gaatataccg ctcgaatctg aattcaccgg cgggctccga tactcgcgat
     7621 tatctggcac gcaggggtct gagtcccgcc accatcgagg agttcgggct gggactgtcc
     7681 ggcaactcct ggcagactct tgcgcggcgc ttcgaacagg aaggcttctc gcgcgagcaa
     7741 atggaagctt cgggactggt cttgcagcgt caggatggta gcggtttttt cgaccggttt
     7801 cgcgggaggc tgatgttccc cattcacgat gaatcggggc gtatcatcgg tttcggcgga
     7861 cgcgccatgg ccgcgggtga cgagtcgaag tatctgaact cgtccgagac ccttctctat
     7921 cgcaagaggc atgtccttta caatctccac cgcgcgaaaa ccagcatccg gaaacaggag
     7981 cgagtgattc tcgtcgaagg gtatatggac gtcatcggtg tcgcggcggc gggtattggc
     8041 gaggtagtcg ccggctgcgg aacttcgctc aaagacacgc aggttcgaat aatacaccgt
     8101 cactgcgaca gggttgtcgt caatttcgac ccggataccg ccggagcgaa tgcggccgaa
     8161 cgttccatcg atatcctgct tggcgaaaaa ctcaacattc ggatcctcga actcgaagag
     8221 gggctggatc cggatgagta catccagcgc ttcggagcga atcgttaccg cgaaaaagcg
     8281 gacaaagcgg ccggctattt ccactggctc gcggaccgtg cccgcaccag acatgacgac
     8341 ggtacggcac agggtaagtg ggcggccttc caggcggtgc tgccggcgat cgtaaagaac
     8401 tccgacaagc tggagcaggc ggcggtggca aacgatatcg catcctatct gggtgtcgac
     8461 gccaacctgg tgcgcgaccg tttgcgtagc gccggctatg aaaaccgtgg ccgcgcggaa
     8521 aagagggctg aatcgaacct tccgcctgtc gaaaggatcc tgttaaatct gctgctcgcc
     8581 ggcggcgggg ttcgcgaaaa gattattccg gaactggttg cgctgccggg aatggaacag
     8641 ttgcaggcac gacggctatt tgaaaccgtc tgcgcgttat atacgcagca gggagatttc
     8701 acctttgaag atgtcgaagc acgcctcgac gacgccggaa aggccctatt gacttcggtg
     8761 atatttgccg atgaagtggg aggagagagt ggttcggtcg aacaggcaag ggcgtgcctg
     8821 agggagttac ggctcggttg ccgcaaaaca gccgaatcgg cattgcgctc gaaaattcga
     8881 gcggcggaaa aggttggaga tataacggaa gcgttgcggc tgacagagga attgagccgt
     8941 ttacagcggg aagtgaacgt gtaacgcgac gttattgtag aatagcggaa acgtagcaaa
     9001 ccgggtgttc actgtacgct cgtgttttag gatatccagt ggcgggtgag gaaaaatacg
     9061 gtcgtctgaa gaacctcgtt gaccttggga aggagaaggg gtttgtcctg tatgacgacg
     9121 tcagcgagtt ccttcccgac gacatagccg aaggtccgga actcgatgac attcttacgg
     9181 atctcgactc ggccggtatc gagattctgg acgagccgaa gaagaacatc gacggcgacg
     9241 actactccga cgccgacctt tctcaggaat tcggcgaaaa gaccaacgac cccgtccgaa
     9301 tgtacctgcg ggagatgggc accgttccgc tgcttacgcg cgacggagag atcgaactgg
     9361 cacgccgcat cgagcatgga cagaaggcgg tccagaaagc cctttcccgt tcgccgttga
     9421 tcattcgaga gatacttcat ctcggttcga ctctcaatcc ggaagacggg tcgattcgcg
     9481 atatcctgtc gcttcccgat ctgctgctca gcgatgaagc ggccgtggaa catgcgctgc
     9541 ggttcctggc ggcgctcgcg gaaatcgaga ctcacttcag gcgcgcgcaa cagtaccgcc
     9601 tgcggatgca gtcggcgtcg agctcattga gaccgaagca gattcgcggg ctgcgtttct
     9661 cactggccag ggaactggta atggtctccc agatggtgcg cgatctccaa ctcagcccca
     9721 tcatccggcg gaggctcgcc ggttgtttgc gccgcacggt agaggaattt cgcgatctag
     9781 agcgcgaact cgcccgcgca cagaggcggt cggaagaagc tgccaacgct tctgcatcgc
     9841 cgcagcggga agggaagcgg gaactccggc aattggccgc ccgcctggag cagcttgaac
     9901 aggagcgtgg cgccagttcc acagagctcc ggcgcacttt gcagatcgtg gagcgtgggg
     9961 aagcggaggc cgagctcgcg aagaagcagc ttattgaggc aaacctccgc ctggtggttt
    10021 ccatcgcgaa gcggtacacc aaccgtgggc tgcaattcct cgacctgata caggaaggca
    10081 acatcggcct gatgaaagcg gtggacaaat tcgattaccg ccgcggctac aagttctcca
    10141 cctacgccac ctggtgggta cgccaggcca ttacgcgcgc cattgcggac caggcccgaa
    10201 ccatccgcat tcccgtgcac atgattgaga ccatcaataa gctggtgagg acccagcgga
    10261 tgctccagca gaaactgggc cgcgacccca ccaccgaaga gctgggcaga gaactcgagt
    10321 tgccggtcgg gaaggttcgt aaagttttac ggatcgctca ggagcctatt tctttggaga
    10381 cgcctgtcgg cgaagaagaa gagtcacacc ttggagattt cattgtcgac cggcgggtaa
    10441 cctcgccatc ggaagcggtc atcaatctca acttgcgcga gcagacggcg gaagtcctca
    10501 aaacgcttag cccgcgcgag gagaagatca tcaaaatgcg gttcggtttg caggacggaa
    10561 gcgaacacac gctcgaagaa gttggccagc acttcgccgt cacgcgggag agaatccggc
    10621 aaatcgaagc caaggctttg cgtaagctgc gccaccccag ccgcagtcac cggctgcgcg
    10681 cgtttctgga tacgtcctgt cacgagtaga ttcgtcttac ctcgtcgcga atagcggccg
    10741 gcacaagatg ctcccacggt tctttgcgtg cgatacttgc gcgcacctgg ctcgcggaac
    10801 acccgctgta atcccggtct aaattcaggt tgtgaattct gctccggaat tccgccgggg
    10861 cttcgtacac accttcgcgc ccggcgacaa gcagttcgaa ctcgccgagc atgcgcgcga
    10921 aagccccagg ctcaccgtag tcccacatga cgatacgttc cgcggcatcg cgcccgcaca
    10981 ggaaataaag ctgtgtggcc gcgccgtacg ccatgcggca ttccctggcg atatcgataa
    11041 acaggcctcc cttcgtcgag gccacggagc acggcgtgcc ttcagccatt cgcagcagca
    11101 ggtgaatgcg gtcctcgaat cccgcgccgg tgtcgtactc tttgtgggga aaggcttcag
    11161 gcagcacata aagcacctca tccaccaggg ggattgctgc cattgccatc gcttcatgcg
    11221 caatcgtcgg cgggttaaac gtacccggaa agatggcgag gcgcgtcatc gaggcgcagg
    11281 cgcgccggaa gaagcgcata tctatccagt gtacggttcc gttgatgccg ttgcggtaaa
    11341 gttagttgga gaaacccatg ttcacctgga tatgccagaa atgcggcgcg gaggttccac
    11401 catcgtattc ggagtgtccg aactgcgctc cgaagccgga aggacaaaag gaaccggaac
    11461 cggtgccggg ggctcccccg ccttcgctcg cagccgcacc gccgccgcag gagaaggaga
    11521 agaaggcaaa gcctgcacag cggccgggct cgaaaccggc actgcccgga tggctggtcg
    11581 cgctgatcgt ggccggcgtg atcaccggta tcggcgcgct cgcgtacttc tactttctgc
    11641 ccgccgtgcg cacgcggtct caggcagcca caccggcgac acggctggag tccgtcccgg
    11701 cgacatccgg gagtccgatt gccaaacacc tggaactcac cggaattcgc atcacggaag
    11761 atacacggaa gcgtgtggaa gtccgcctga ttgttgtcaa tcactccgcc gccgagattc
    11821 ccgacctgga gttgtcggtg gacctgaaac cgtcgaatgc ttcaccggaa accaaaccgg
    11881 tggccagttt cgatatgaaa gttccggggc tggggccttt cgaggccaag gaggtagtga
    11941 caaccgcaaa aaccgaactg cgggcttacg agattccgga ctggcagttc ctggcggtga
    12001 acttccgcgt gatgtcaccg aaatgagggc gctgccacta tgttgacgcc ggtaagacct
    12061 gtcaagccga agacgcgcgc cgagcgcagg ctgcgcgtgg aggcgatcct ttccaagctc
    12121 gacgagatgt accccgcggc cacctgcgcg ctggtgcatc acacgccatg ggagctcctg
    12181 gtggccacca tcctctctgc gcagtgcacg gacaagcgtg taaatgaggt cactcccgaa
    12241 ctgttccgca agtatcccac cctcgaagac tttgccgcgg tgagccaggc ggagttggcc
    12301 cgcgatatcc gttcgacggg atttttcaac aacaaagcta aatcgctcat tggagccgcg
    12361 cgaaaaatca ttaccgattt cggcggcgag gtcccacgaa ccatggacga gatgctcacc
    12421 ataccgggag cagcgcggaa gacggccaac gtggttctcg gcaccgcatt cggaattgcc
    12481 tccggaatcg ttgtggacac gcacgtccag cgggtttcgc ggcggctgga tcttacgcgc
    12541 aacaccgagc cggttaagat cgaacaggac ctggtaaaga ccattccgaa agagaaatgg
    12601 attctgttct cccaccagat catccatcac ggccgcgcac tctgtatcgc gcggaagccg
    12661 aaatgtgccg aatgcggcct ctacagtctc tgctattcga aagataagac ggcgtagtga
    12721 cgttctcttg ccggcgaaca tgagcgcaag tcacttgcgc gcaatcaggc ggaatatttc
    12781 ggtcttccgg cgatcggaga agtccgagac ggcgtgcgtc atttcgtacc gctcgatgag
    12841 gaggccgtcg aacaggcgaa gcatgccatt tcgcccaaca aagccgggac caaactccat
    12901 cactactatg cctcccggct taagtgcatc aatgaccttg tggacatcca cgagcggcat
    12961 cgaccaactg aacaaaatca ggtcaaagcg ttctttgcca aaattgtagt tagcgtcggt
    13021 agacactacg gcatcgatct tcagaccggc ttcagcggcc cggtgttttg ctatatcgat
    13081 cgcaacctcg gacatgtcga agccgtgaac ggtccagcca agccgggcaa gatagatcgc
    13141 gttgcggccg tcccccatgg cgtagtcgag tgcggtgccg ggcttcatgt cctccactac
    13201 tactgtcaga aaagagtttg gcgcccggtt atagttgttc ttgggatcgc tgtaaaatcg
    13261 gttccaccgg tcggcctcga gtagatgccg gtgattctta agcaaatcga gccggcggac
    13321 aatctctttt tcaggtacac cagccgtacg tagttcggcg gcgtagacct ctggcttctt
    13381 gtcgcgggca tcgcggtaga aatcgaattc gggcttctgt ccggcaatgg gtaatgaaag
    13441 caacagaacg gcgcagagga gcttcatcgg tggtgttaga tgcggcaagc agaccccaag
    13501 ttaccgttcc atatcacagg tggaccctag ctcactccca actcggctgc cgccacccgg
    13561 agccgcttgt cgagagtcca caggcggcaa tgagagatga gcgctgaagc gagcaagtgg
    13621 gcatcgatcc agccgagccc tcggccccat agtctgcggt cttcgacgag gcgcatcact
    13681 tcggcatcgg aagcatgtgt ggccagtggc agtgcggcca agtctgccag gatggcggcc
    13741 cgttttctca ggttgccgca tgccatctcc cctgtcacga acggatgcat aacaaccaga
    13801 ccattccata ggagatccgc gagaacaggc tgtcgcttgc gcagatgagc gatccagacg
    13861 gaggtatcca cgagcaccat caggcgcttc gccgtcgcgg gatgccggaa attccgggtt
    13921 gggtgccgcc gagtgccgcc aggcgtttgc ctgcctcacg tgcgatgagg gcctccaaac
    13981 ccgccctgac caaagcagtc ttctcctgaa tgcccgttgc ctccctggcc cgctccaaaa
    14041 gagcatcgtc gatgatgatg gtagtcctca tgcatattag tatgcatcat ctatgcagcc
    14101 ggctggacgt catcgttggc actgctcaca agcgcacagg cgaaggcacg ctggtcgtct
    14161 tattcttgat ccggaactgg caggatgcgt gcgagttcat cctcgacatc gactttgtag
    14221 ctggcagcga gcagggcaat ctgcggcccg atgcagccac gcaactcgcc gagtgccgag
    14281 tagaaatcga acgactccat accatggtgc aggcgcaccc ggcgatgtcc atcatggtgc
    14341 attcggtcca ggaatttccg gcaggcggca cggatggcac ggaacggcga cagaagatcg
    14401 ctcccgtgtg ttaggtccgc gatctcgctc gttaggaagc ggcggatctc caggacggac
    14461 tcaatgcagt gattgggctc ctcccaggcg taatcgctgt acaggacgcg acggtcctca
    14521 aggaactgta tcacgcgtcg ggcaacctcg ctatcggtgg gtttaggcgt ccactggact
    14581 ccgatggaag cgatcgaaaa cccgttcagc cgattggcgg cttcgttcca tttcatactg
    14641 gactccgatc atagcgcctg atgatcggtt gctggggcgc ggcacccctc tccggggttg
    14701 ttgccaacga taaattactt gactgggcgt cgcccaattg aacggcgcag cctgtgactt
    14761 ttccgaactc cctttacgga gctgaacgcc ttctgtccgg agcgtcgcgg ccacgttgag
    14821 gagccggctt gggcgtgtcc ttctccggtt ctgctttcag ttcacgccgg gacccggcgt
    14881 cgcaggattg cgacattcaa gtgggaaccc ccagccgtgg tagcacccaa cgcatcacga
    14941 ccacgtaggc ggccagaatc aggatcagcc ataatgaatc catagttcgc tttcgcacac
    15001 cagcactagt gccgatgcag gttttccggc gaggtgacaa tttgtgttac ctccgccgcc
    15061 gggtttcatc ttaacgagca ggactcatgc gggaagacaa gaacaagaga tggcagacgg
    15121 cactgaagct gttgctggtg gcggtgattg taggactgat ctcgttcggt ctggatctcg
    15181 gctcggcggt ggatacgcat acttatgcgc cgggggtccg gaaaccgatg ccgcatttcg
    15241 agtacaggga tctttccggc gccacctgga acctcgatga gcagcgcgga aaagcactgc
    15301 tggtgaactt ctgggccaca tggtgcccgc cttgccgccg tgagacgccc gacctggtga
    15361 agctttcacg agagtacagc caggaagagt ttgtaattat cggggtgtcg ctcgacgacg
    15421 gggcagagca ggtccgccgc ttcgtctcgc aattcgatgt tccctatccg gtggtgatgc
    15481 cgcgagagaa cgaccccctc agcgcccaga ttcagtcgct tccaacttcg attctggtgg
    15541 acccggaggg gcgtatcgcg cggatgtacg tcggcgccgt ctccgcatcg caactgcgaa
    15601 aagatctcca gcaattgttc ggggaattcc ggcctacttc ctagacaatt tatccgtttg
    15661 cggatggcgc gccaggttgc tgtgataccg gctgtaagtg aaataaatca ccaggccaat
    15721 gaccagccac acgaaaaggc gcaaccagtt ctcccatccc agtccgtaca ttagggcgag
    15781 attcaccagg atgccgagga gcggcaccag cgggacaaga ggagtgcgga atggccttgc
    15841 aagatccgga tgcgtccggc gcatgaccag aatgcccgcg caaaccaggc tgaatgcgaa
    15901 cagcgttcca atactggtca tgtgaccgac aaccgagatc ggcgcgaagg cggaaaagag
    15961 gctcacaaac accatgaaca gcatgttcga ccggtagggc gtgctccatt tcgggtgaat
    16021 gtcggagaag atcttcggca acagcccgtc tct
//
LOCUS       DASTMK010000488         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_567598, whole genome shotgun sequence.
ACCESSION   DASTMK010000488 DASTMK010000000
VERSION     DASTMK010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567598"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="VFQ79_20450"
     CDS             complement(<1..2123)
                     /locus_tag="VFQ79_20450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142105.1"
                     /translation="MQSLLATLFLAFAIVLSGTGKALYAWQPSPKEGLSENSKPESTE
                     PPKPASEVSRRTELNLLGKTDTAAGESRRNENIQFNLVDNNALKELNVRLGTTVTVIQ
                     EFQPDRSYFGAEFGVAPRSPLHLGAGAFDKIHGRLHASHLNSIFSARSFFQVGDVQAA
                     HENDYGFRLSSPVWSGAYLSLEGSQQKIRGSVNGNVLVPRPDERTPLATDPAIRAFVA
                     RLLAAYPLALPNRTDINERALNTNAPQHINSDTGAIRLQQDVGARDRFLFSYLFTSQS
                     VDAFQLVSGQNPDTDTRAHTARITWNRNWTATTFTDFSAGFDRVGSLLVPEENAVGPN
                     ISISGLTSLGPESAIPINRAMNMFRYEGMIRQLRGNHTWTAGFGLLRRQFNGIESDTH
                     RGFFSFTNDFGRSSIDNFRLGLATQHIRAIGNVHRGFRNWDMQYYAGDTWRVRPDLTL
                     NLGLRYQPVTTPSEVNRFNIIPYGCDCNNVAPRFGLAYRMPSWLGTLRAAYGLDYGEI
                     FPVTFGQVRFAPPVNHKIVIPSPYLLDPLGSLTHSGGVPDALSTTYVLDPELAVPYSH
                     QYNFTWEPALASDWRIQLGYVGSRSHKLLLMWYTNRSHPVPGIPQTTATVNSRRADPR
                     FAEIRRVINGSRGYFDAARLSLLLPRWRRLSMDAAFWYSKALDLGSSYSNTAYDQDSR
                     LSRSQSEFLQDADMKGPSSFDQPFA"
     gene            2853..4037
                     /locus_tag="VFQ79_20455"
     CDS             2853..4037
                     /locus_tag="VFQ79_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEU0142106.1"
                     /translation="MAHQPDSNVIETVVQLLCESGLSQMAEAVRIMLNEAMKIERSQV
                     LAADPYERSERRQGYANGFKPKTVETRLGAMTLQVPQARGVEFYPSALEKGVRSERAL
                     KLAVAEMYVQGVSTRRVTEVMQQLCGLEISSTQVSRAAKLLDEELTAWRQRAIGEIPY
                     LVLDARYEKVRHGGSVVSCAVLIAVGITPDGHRALLGVSVSLSEAEVHWREFLGSLQD
                     RGLHGVRMIVSDDHAGLKAAREARFAGVPWRRCQFHLQQNAGHYVPRISMRGEVAADM
                     RAIFDAPDREEADRRLKLAVQKYEKTAPKLASWMAANIPEGLTVFTLPPQHRRRLRTS
                     NLLERLNKEIKRRTRVATLFPNEAALLRLVSAVLMEISEEWETEKIYLRMENSGSTTR
                     EE"
     gene            complement(4339..4944)
                     /locus_tag="VFQ79_20460"
     CDS             complement(4339..4944)
                     /locus_tag="VFQ79_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142107.1"
                     /translation="MLTLIAGMTAAIFFATAIWAGEDAGNQRLERKIRVNGRPLGPDE
                     MTRLEEAESRYRFRLPDADLWYDARSGAVGLWKGPTAGFMPAGLKLGPKMPANCSTGG
                     TGVFVNGRELHPIDVMTLRQFMTVWPGRWWVDAYGNGGLDGGPAMFNMVSLAQQAHSR
                     GSGNSAWSRRVDAGGGSMTAGGDGGFFYFMDSNGNTAYLGN"
     gene            5063..>5664
                     /locus_tag="VFQ79_20465"
     CDS             5063..>5664
                     /locus_tag="VFQ79_20465"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU0142108.1"
                     /translation="MRSIILLFLITAAGFGQTAEKTIIVRPAEIDDVLVNPGIGFMTF
                     QRFNGDALNEGLKWTEGYPIVYQEFKGSLENKNHPMTSIAYFRVYWKFLEPEQGKYRF
                     DLLDTALKTAHNRKQTLMLRIAPYGTGKDNDVPDWYRTMMGPEPGLKIAKWRTNPEDP
                     RYAQHFGKLIREIGKRYDGHPDVESIDLAIVGAWGEGAGAV"
BASE COUNT         1235 a         1611 c         1709 g         1109 t
ORIGIN      
        1 aaggcaaacg gctggtcgaa cgagcttggg cccttcatgt cagcatcctg cagaaactcg
       61 gactggcttc gagacaggcg cgagtcctga tcgtaggcag tgttcgaata actgctgccg
      121 agatcgagtg ccttgctata ccagaacgcc gcgtccatgg ataaacggcg ccagcgcggc
      181 agcagaagcg acagacgggc cgcatcgaaa tagccgcggg agccgttgat aacgcgccgg
      241 atctcggcga atcgcgggtc ggcgcggcgc gaattcacgg tcgccgtggt ctgtggaatc
      301 ccgggaaccg gatgcgaccg gttggtgtac cacatcagca gcagcttgtg cgaccggctg
      361 cccacatagc caagctgaat gcgccagtcg cttgcaaggg ccggctccca ggtgaagttg
      421 tactggtgcg agtacggcac cgccagttcg ggatcgagca cgtaagtggt gctcagcgca
      481 tccggaacac cgccggagtg agtcagagag ccgagcgggt ccagcaggta aggcgaggga
      541 ataacgatct tgtgattcac cggtggagca aagcgcacct gcccgaaggt aacaggaaag
      601 atctcaccat agtccagccc gtaggcggcc cgcagcgtgc ccagccagga cggcatcctg
      661 taggcaagtc cgaaacgcgg agcaacgttg ttgcagtcgc agccgtacgg gatgatgttg
      721 aagcggttca cttccgaagg agttgtcacc ggctgatagc gcaggccgag gttgagcgtc
      781 agatccggcc gcacacgcca cgtgtctccg gcgtaatact gcatgtccca gttccggaat
      841 ccgcggtgga cattgccgat ggcccgtata tgctgcgtcg caaggccgag ccggaagtta
      901 tcgatggagc ttctgccgaa gtcgttagtg aacgagaaga agccgcgatg cgtgtcggat
      961 tcgattccgt tgaactgccg gcgtaacagc ccgaacccgg ccgtccaggt gtggttgccc
     1021 cgaagctggc gaatcattcc ctcgtaccgg aacatattca tcgcccggtt gatgggaatg
     1081 gcggattccg gacccaacga ggtaagcccg ctgatagaaa tgttggggcc gacggcattc
     1141 tcttcgggca ccagcagcga tccgacgcgg tcgaaaccag cggagaagtc cgtgaaagtg
     1201 gtggctgtcc aattgcggtt ccacgtaatg cgcgccgtgt gagcgcgcgt gtcggtgtcg
     1261 ggattctgtc cggagaccag ttgaaatgcg tcgacgcttt gcgaggtaaa cagataggaa
     1321 aacaggaagc ggtcccgcgc tcccacatcc tgctgcaggc gtatggcgcc ggtatcgcta
     1381 ttgatgtgtt gcggagcgtt ggtgttcagc gcgcgttcgt tgatgtcggt ccggttcgga
     1441 agcgcgaggg gataggccgc gagaaggcgc gcgacgaacg cgcgaatggc gggatcggtg
     1501 gccagtggtg tccgctcatc ggggcgcggc accagcacgt ttccattcac actgccgcgg
     1561 atcttctgtt ggctgccctc aagtgaaagg tacgccccgc tccagacggg tgaagagagg
     1621 cggaatccgt aatcgttttc atgcgcggcc tgcacgtcgc ccacctggaa aaacgaacgt
     1681 gcgctgaaga tgctgttgag gtgtgatgcg tgcaaccggc cgtgaatttt gtcgaacgct
     1741 ccggcgccaa gatgtagcgg gctgcgggga gccactccga actccgcgcc aaaataactg
     1801 cggtccggtt ggaactcctg tatcaccgtc acggttgtgc caaggcgtac gttcagttcc
     1861 ttgagcgcgt tgttatcgac gaggttgaac tgaatattct cgttgcgcct gctttcgccc
     1921 gcggccgtat cggtctttcc cagcagattc agttcggtgc gccgggaaac ttcggaagcg
     1981 ggcttcggcg gttccgtact ctcgggcttg gaattttcag acaggccctc tttgggcgag
     2041 ggctgccagg cgtataaagc ttttccggtt cccgacagga caatggcaaa agcgagaaac
     2101 aacgtagcca gaagagactg catggtcacc cagcaacata gcagacctcc gggcgccgcc
     2161 gtgcagcggc ccgatctgcc cttggtgcgg ttcgaaagag aacttctata cgcctagccg
     2221 ccgtggtgga agtgcaagac cttgtcgctc gcggttttca ccaaaaccat ttgcgaagac
     2281 tccgcaatcg gctttcacaa gtggttgacg gcgatggatg atcgccactg acaagaacgg
     2341 tatcagttgt gggaactcca ccctagtctt gatgtcacct caaaaggccc tgtggttcat
     2401 gcttcagcgc atccggctgg cgatgcagac cgggacgttc gaaaaaccgt aggaagggtg
     2461 gaagtagaca agatattttc ggcagtaaag cctggaacat ggacaaacac atccaggaac
     2521 agaagatcat cgggattggc aacacggaca agacgatggt gattggcctt ctcgaacgtg
     2581 gtggcggatt agcgtcttca ggaacctccg aaagaaaagt tggtgtaaca tcctttctgt
     2641 aaattcgact cggaccgaga gcgacataga ttgagttcag gccggagttc cccggtttga
     2701 gggttgagtt cttgttctgg gtcggagcga aggggcgcgc agttcagcgc cccgtagcgg
     2761 aggcccagaa caagaactgc cggccttgtt tcggcgggcc aaatctgttt tcctttcttg
     2821 agaactgcga attcttcgga aaggagaccg acgtggccca ccagcccgac tctaacgtaa
     2881 tcgaaacggt tgttcaactg ctttgtgaat ccggcctgtc gcagatggcc gaggccgtcc
     2941 ggatcatgct caatgaggcg atgaagatcg aacgctccca ggtgctcgcc gctgacccct
     3001 acgaacgtag cgaacgccgc cagggatacg ccaatggttt caagccgaag accgtggaaa
     3061 cgcggttggg tgcgatgact ctccaggttc cgcaggcccg cggcgttgag ttctatccct
     3121 ccgctctgga gaaaggcgtc cgcagcgagc gagcgctcaa actggcggtt gccgaaatgt
     3181 atgtccaggg tgtatccaca cggcgggtta ccgaggtgat gcagcagctt tgcgggctgg
     3241 aaatctccag cacgcaagtg agccgagctg cgaaactgct ggatgaagaa ctgaccgcct
     3301 ggcgacagcg cgctatagga gagattccct acctggtcct ggatgcccgc tacgagaaag
     3361 tacgccacgg gggctcggtg gtatcctgcg ctgtcctcat cgccgttggg atcaccccgg
     3421 atggccatcg tgcgttgctg ggcgtgagcg tctctctcag cgaagccgag gtccactggc
     3481 gggagtttct cggcagcctg caggatcgcg gtttgcacgg agtcaggatg atcgtcagcg
     3541 atgaccatgc cggtctgaaa gcggcccggg aggcgcggtt cgctggcgtg ccttggcggc
     3601 gctgtcagtt tcacctgcag cagaacgccg gccactatgt gccgcgcatt tccatgcgtg
     3661 gcgaagtggc cgccgatatg cgcgccatct tcgatgcgcc ggatcgagag gaagccgatc
     3721 gccggttgaa actggccgtc cagaaatatg aaaagaccgc tcccaaactc gccagttgga
     3781 tggcggccaa catacctgaa ggcctgaccg tcttcacgct gccgccgcag caccggcgcc
     3841 gcttgcggac ctccaatctg ctcgagcggc tcaataagga aatcaaacgg cgcacccggg
     3901 tagccactct gttcccgaac gaagccgccc tgcttcgcct agtcagcgcc gtcttaatgg
     3961 aaattagcga ggagtgggaa accgaaaaga tctacttacg aatggaaaac agtggctcga
     4021 ctacccggga agaatgaatt gacagaaagg ggcttgcttt atcttttctt cctacctgcg
     4081 tctctgatag atagcatcag ttcactcaaa caatgggcgt agtgggatca taggatccta
     4141 tctattctgc ctaatgcaag ctgcaaagga gcttatcaac tctgccatct tcgatcagct
     4201 tctttcctac caaacaatcg acgttataaa tgttattaac tcaactcgat acagaccacc
     4261 ttctcccggc gaacggacac acttatgtcc catgccgcca cgagcggtca acgctcgggt
     4321 ctggtgacat caagtatgtc aattgcccag atatgcggta tttccgttgc tgtccatgaa
     4381 gtaaaaaaag ccgccgtcgc cgccggcagt catgctgcct ccgcccgcat ccacacgcct
     4441 gctccacgcc gagttaccgc tgccgcgcga gtgcgcctgc tgcgccaaac tcaccatgtt
     4501 gaacatcgcc ggtccaccgt cgagccctcc gttaccgtat gcgtcgaccc accaccggcc
     4561 aggccacacc gtcatgaatt gacgaagagt catcacgtcg atcggatgca gttcgcggcc
     4621 gttgacaaaa acaccggtgc cgccggtgga acagttcgca ggcatcttcg gccccagctt
     4681 aagtcccgca ggcataaatc cggcagtggg gcctttccat aagccaaccg cgccgctgcg
     4741 ggcgtcatac cagagatcgg cgtcgggaag acggaatcga taccggctct cggcctcttc
     4801 caggcgggtc atctcgtcgg gtcctagggg ccgcccgttg actctgattt tccgctccaa
     4861 ccgctggttg ccggcgtctt ccccggccca gatcgccgta gcaaagaata tggccgcggt
     4921 catccctgca ataagagtca gcatccgtca gcccctcctt tgctacgaaa cgcgaattca
     4981 cctgactgga accgtcaaaa tcgcgagcaa agcggtactc ggagataccg ctcagggcgc
     5041 agtgtagtat aacggccttg agatgcgaag cataatactt cttttcctga ttaccgccgc
     5101 cggattcggc cagacggcgg aaaaaacaat catcgtgaga ccggccgaaa tcgacgacgt
     5161 attagtaaat cccggaattg gcttcatgac ctttcaaagg ttcaatggcg atgccttgaa
     5221 cgaaggcttg aagtggaccg agggataccc catcgtttac caggagttca agggaagtct
     5281 cgaaaacaaa aaccacccga tgacttccat cgcttatttc cgcgtgtatt ggaaatttct
     5341 ggaaccggag cagggcaagt accggttcga cctgctggat acggcgctca agaccgcgca
     5401 caaccgcaaa cagacactga tgctgcgcat agcgccctat ggcacgggga aggacaacga
     5461 cgtgcccgat tggtatcgca ccatgatggg gcccgagccc ggactgaaga tcgcgaagtg
     5521 gcgcacgaac cccgaagatc cacggtacgc gcagcatttc ggaaagctga tccgggagat
     5581 agggaagcgc tacgacgggc acccggatgt ggagagcatc gatcttgcga tcgtcggcgc
     5641 gtggggcgaa ggcgccggcg ccgt
//
LOCUS       DASTMK010000489         5252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_87978, whole genome shotgun sequence.
ACCESSION   DASTMK010000489 DASTMK010000000
VERSION     DASTMK010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87978"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFQ79_20470"
     CDS             <1..562
                     /locus_tag="VFQ79_20470"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEU0142109.1"
                     /translation="GMSMCFLSGNALCQELVTYESTVTGKPLRTFARKKRFEDEDLLM
                     GLKSYGPGYGDWTVTRADHWMFEGTGMKNGDSIAGLIGWEFHGTPSKKIEGLVEVARS
                     KLSPRSVRPGRDADGWHSAVIYPCAKGNWVFNAGTIWWPEGLAMPPGHAPAGSQIART
                     QGPDERVQRITTNLLQRMLRDSPVRL"
     gene            624..2096
                     /locus_tag="VFQ79_20475"
     CDS             624..2096
                     /locus_tag="VFQ79_20475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0142110.1"
                     /translation="MKRRQFLAGAGAAPLLGAAPAQSAPKRNLVLFIVDAMRSEMMGC
                     AGNKVLQTPNIDRLASRGTIFTNAFCPHGVCMPNRASIFTGRYPGAHGVWANGVPLSK
                     NERTLAHVLDENGWATGSAGKHHFEAELAPQYPPRLKPGESYYGFRETHLAENVPGAG
                     YIAYLKKNFPQYSDSFRKVTLPPEAHQIHWIVSQTENFLSRQRKAGSPFFAYCSFPDL
                     SPRDTPPLGFDKMYQPADMPMPQRRAGELDRKPPHYRKVYENQLAKKRYPNDEQYREL
                     MALYYGQMSFVDQQLGRLIRAVEQAGQIENTMFVIATDHGLCLGDHWIWRHGPWMYDQ
                     VTHVPLVISCPWVSGPRVVREMVEHVDILPTILDCLGAPVPEGVQGRSLRALLAGEAG
                     ARGKDTVWADDREAYELEASGISHQGFHVMSVRSRNWKYVHYAGKPYGELYDLRNDPD
                     EFENLWDSPDHRGLRNECRDVFLERLIATRDPLPAPVAHW"
     gene            complement(2132..4258)
                     /locus_tag="VFQ79_20480"
     CDS             complement(2132..4258)
                     /locus_tag="VFQ79_20480"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0142111.1"
                     /translation="MRTLFVIVSGFLWAAGSIAAQSADSLDFVTTHTDFREIRQMLGK
                     HVRAKAFEQLDARKQEVARISSASEVAARKKTIRERVLRAIGGLPERTPLNARTTGVI
                     ERDAYRIEKIVFESQPKFYVTANLYVPKRGQPPYPAVLYPLGHETGAKANPVWQQMLG
                     SLATKGYVALTWDTLGQGERIQLYDEDFRRSKLVQSTTEHTVQGTQCLLVGDTLARYT
                     IWDGIRALDYLLSRKEVDPKRIACSGNSGGGTHTTYLTALDDRIQVAAPSCYITTWRR
                     LLETIGPQDAEQCMPPFLAVGLDIPDFIHAAAPRPFLVLSAIRDFFSITGARQAYAES
                     KRIYEVAGAAGKINMVEADDGHGFTKPRRMAAYDWISRWLKDTPDTAPEPEIEIASEA
                     ELFATPGGQVTTSLGGETVFTLNRARAQQLQRRDAALNADAVRAAIGFERVTGAVPVR
                     AFGTIDRPGYTIEKLVYESEPGISIPSVLFVPKEAAGPRPAVIFVDGRGKSAAREQLE
                     SLATSGLAVLAIDARGFGETSTRAGEGSSEWSRSFGDYDSGMTALLTGRSLVGMRTLD
                     IVRAVDLLAARDGIDSNRIYAVGVSGGAVPLLHAAFLDPRIRRVALDGMLSSYITVVN
                     QRINRGVFEQVVQGVLKVYDLPDLVASLAPRSVRITDLVDAAGAPVPLAEAQAQYKRA
                     PAESLRITQRRLEEPPLAAYRDLLVQ"
     gene            complement(4373..5086)
                     /locus_tag="VFQ79_20485"
     CDS             complement(4373..5086)
                     /locus_tag="VFQ79_20485"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEU0142112.1"
                     /translation="MLASAAPFYAQSPARVSDINWQGWFAYNGDHALSNRWSIHFDTQ
                     ARRHPFVTKWQQYYVRPGVNFQLNGLIRVGVGYMFGKSYPYGDYPAQRASHEHRIYEQ
                     LEIRHKTGDLKWGHRFRLEQRFAENGVTHKDEKKVIDWVYRNRLRYRLHLQIPLAGET
                     WYLPLSNEIFLNFGANSPRAFNQNRAYAGIGHKLSETASLEVGYIYQTQLQNSGRVME
                     HNNTIIISFSSSFPFRRSN"
BASE COUNT         1067 a         1540 c         1641 g         1004 t
ORIGIN      
        1 gggtatgagc atgtgctttc tctcgggaaa tgcgctctgc caggaactgg tgacttatga
       61 atccaccgtc accgggaagc cgctgcgcac ttttgcccgc aagaaacggt tcgaagacga
      121 agacctgctg atggggctga aatcctatgg acccggctac ggcgattgga cggtgacccg
      181 cgcggatcat tggatgttcg aggggaccgg gatgaagaac ggcgactcga tcgccggcct
      241 gattggctgg gaattccacg gcacgccgag caaaaagata gaggggctgg tcgaggtggc
      301 ccggtcgaag ctgagtcctc gcagtgtccg tcctggccgt gacgcggatg ggtggcacag
      361 cgccgtgatc tatccctgtg ccaaagggaa ttgggtcttc aacgcgggca ctatctggtg
      421 gccggaagga ctcgccatgc cgccgggcca tgctccggcc ggttcgcaga tcgcccgtac
      481 gcaagggccg gacgagcggg tgcaacgcat cacgacgaat cttcttcagc gaatgctgag
      541 ggattcaccg gtccggcttt aggagcttgt ttgagaaatc gcttgctgac gcgcgcggct
      601 cagaatttta cgggagcggg cctatgaagc gccgccagtt tttagccggt gcgggagcgg
      661 caccgctgct gggagccgct cccgcgcagt ccgcgccgaa acgcaacctg gtcctgttca
      721 tcgtggacgc gatgcgttcg gagatgatgg ggtgcgccgg caacaaggtg ctccagacgc
      781 cgaatatcga ccgccttgca agccgcggga cgatttttac caacgccttc tgcccgcacg
      841 gtgtctgtat gcccaaccgg gcttcgatct tcaccggacg gtatccggga gcgcacggcg
      901 tctgggcgaa cggcgtgcct ctcagcaaga acgagagaac cctcgcgcat gttctggacg
      961 agaacggctg ggcgacaggc tcggcgggga agcaccactt tgaggcggaa ctggcgcctc
     1021 agtatccgcc gcggcttaaa cccggtgaaa gctactatgg cttccgcgaa acgcatctgg
     1081 ccgaaaatgt tccaggcgcc ggatacatcg cgtatctgaa aaagaacttc ccgcagtata
     1141 gcgactcgtt ccggaaagtg acccttcctc cggaggcgca ccagatccat tggatcgtca
     1201 gccagacgga gaacttcctg agccggcaga ggaaggcggg ctctcccttc tttgcctatt
     1261 gctcgtttcc cgatctgagc ccgcgcgaca cgcctccact gggcttcgac aaaatgtacc
     1321 agccggccga tatgccgatg ccgcagcgcc gcgccggaga actcgacagg aagccgccgc
     1381 actaccgcaa agtctacgaa aaccaactcg cgaagaagcg atatccgaac gacgaacagt
     1441 atcgcgagtt gatggcgctc tactacggcc agatgtcgtt cgtcgatcaa caattggggc
     1501 ggctgattcg cgccgttgaa caggcgggcc agatcgaaaa cacgatgttc gtgattgcga
     1561 ccgaccatgg cctttgcctc ggagatcact ggatctggcg gcacggtccc tggatgtacg
     1621 atcaggtgac acatgtgcct ctcgtcattt cctgcccgtg ggtgagcgga cctcgcgtag
     1681 tgcgggagat ggtggagcat gtcgacatcc tgccgacgat tctcgattgc ctgggagcgc
     1741 ctgtcccgga aggagtgcag ggccgctcgc tgcgagcttt gctggccgga gaggccggag
     1801 ctcgcggaaa agacacggtg tgggcggacg accgtgaagc gtatgagctg gaggccagcg
     1861 gaatcagcca ccagggcttt catgtgatgt cggtccgcag caggaactgg aaatacgttc
     1921 actacgcggg aaagccctac ggggaactgt acgaccttcg gaacgacccg gacgaattcg
     1981 agaacctgtg ggactcgccg gaccaccgcg gccttcgaaa cgagtgccgc gacgtattcc
     2041 tcgaacgcct gattgccacc cgcgatcctt tacccgcacc cgtggcgcac tggtgagaaa
     2101 accgcttgct cgcgcgcgcg gctcagtccg attactggac cagcaagtcg cgataggcgg
     2161 cgagcggcgg ctcttccagc cgcctttggg tgattcgcaa ggactccgcc ggagcgcgtt
     2221 tgtattgggc ctgtgcttcg gcgagcggaa cgggcgcgcc ggcggcatcc accaggtccg
     2281 tgatgcgcac ggaccgcggc gcaagggaag ccaccaggtc cggcaggtca tagaccttca
     2341 ggacgccctg cactacctgt tcgaagacgc cgcggttgat gcgctggttt accaccgtaa
     2401 tgtaggaaga caacatgccg tcgagcgcca cgcgccggat acgcggatcg agaaacgccg
     2461 catgcaggag cggaaccgcc cctccggaaa cgccaaccgc gtagatacga ttcgaatcga
     2521 tcccatcgcg ggcggccagc aggtccacgg cgcgaacgat gtcgagggtc cgcattccga
     2581 cgagggaacg gcccgtgagc aaagcagtca tcccggagtc gtagtcgccg aaagatcgcg
     2641 accactcgga agagccttcg ccggcgcggg tgctggtttc tccgaagccg cgggcgtcaa
     2701 tagcgagcac cgcgagccca cttgtggcga gactctcaag ttgttcgcgc gcggcggatt
     2761 tacccctacc atcgacgaag ataactgccg gccgcgggcc cgctgcttcc ttgggaacaa
     2821 ataacacgga cggaatcgag atgcctggtt cactctcgta caccagtttc tcgatggtgt
     2881 agccgggccg gtcgatggtg ccgaatgcgc gtaccgggac ggcgcccgtc actctctcaa
     2941 atccgatggc cgctcgaacg gcatcggcat tgagagcagc atcgcgccgc tgcaactgtt
     3001 gcgcccgggc gcggttcagc gtgaatacgg tctcgccgcc tagcgacgtt gttacctgcc
     3061 cgccgggtgt ggcgaacaat tccgcctcgg aagcaatctc gatctcgggt tcgggagcgg
     3121 tatcgggggt atccttcaac cagcggctga tccagtcata cgccgccatc cgtctgggct
     3181 tggtgaatcc atgtccatcg tcggcctcca ccatattgat tttgccggcc gcgccggcga
     3241 cctcatagat gcgcttcgat tcggcgtaag cctggcgcgc tccggtgatg gaaaagaaat
     3301 cgcggatggc cgacagcacc aggaacgggc gcggcgcggc cgcgtggatg aaatccggaa
     3361 tgtcgaggcc gaccgccaaa aacggtggca tacactgctc ggcgtcctgc gggccgatgg
     3421 tttcgagaag acgacgccag gtggtgatgt agcaggacgg cgccgccacc tggatacggt
     3481 cgtcgagagc ggtgagatag gtggtgtgtg ttccaccgcc ggaatttccc gaacaggcga
     3541 tacgcttcgg atccacctct ttgcgcgaca gcaggtaatc gagcgcgcgg atgccatccc
     3601 aaatggtgta gcgggccaag gtgtcgccca ccagaagaca ctgcgtgcct tgtacggtat
     3661 gttccgtggt ggactgcacc agtttcgacc gcctgaagtc ctcatcgtag agctggatgc
     3721 gctcgccctg gcccaacgtg tcccatgtca gggcaacata tcccttcgtg gcgagcgagc
     3781 cgagcatctg ctgccacacg gggttcgctt tcgcgcccgt ttcatgcccc aacggataaa
     3841 gcacggccgg gtagggcggc tgcccgcgtt tcggcacgta caggtttgcc gtcacgtaaa
     3901 acttcggctg gctttcgaag acgatttttt cgatgcgata agcatcgcgc tcgataacgc
     3961 cggtggtgcg cgcattcaac ggagtacgtt ccggcaatcc gccgatcgcc cgcagcacgc
     4021 gttcccggat cgtttttttg cgcgcggcga cctcggatgc ggaggagatc cgtgccacct
     4081 cctgcttacg cgcgtcaagc tgctcaaacg ctttggcccg gacatgcttg ccgagcatct
     4141 gacggatctc ccggaaatcg gtgtgagtgg tcacgaaatc caggctgtcc gcactctgcg
     4201 ccgctatgga accggcagcc cagagaaatc cactgacgat gacaaaaagg gtgcgcatga
     4261 ttctcccgac gaagctaaca tcatcgcact aatgtggtgt caccaccgct tgctcacgcg
     4321 cgcggctcag tttcggagcc gcgacggcca cggagtggtt ggagatagga cattagttgg
     4381 accggcggaa cggaaagctg gaggaaaagc tgatgatgat cgtgttgttg tgctccataa
     4441 cgcgcccgct gttttgcaat tgggtctgat agatgtagcc cacttcgagc gacgcggtct
     4501 cactgagctt gtggccgatt ccggcgtatg cacgattctg gttgaacgcc cttggagaat
     4561 tcgctccgaa gttgagaaag atctcgttcg acaaaggcaa ataccaggtc tcaccagcaa
     4621 gcggaatttg gagatgcagc ctgtagcgga gacggttgcg atagacccag tctatgacct
     4681 tcttctcgtc cttgtgtgta acgccgttct ccgcaaacct ctgttccaac cggaagcgat
     4741 gcccccattt caggtctccc gtcttgtgcc tgatctccag ttgctcgtag atgcggtgtt
     4801 cgtgggaggc acgctgggcc gggtagtccc cgtatggata cgacttgccg aacatatatc
     4861 ccacgccgac cctgatcaaa ccgttgagct gaaagttgac gcccgggcgg acgtaatact
     4921 gctgccactt ggtgacgaac ggatgacggc gcgcttgcgt gtcgaagtgg atgctccaac
     4981 ggttggagag agcatggtct ccgttgtagg cgaaccagcc ttgccagttg atatcggaga
     5041 ctcgcgccgg tgattgggcg tagaagggcg ccgcggaggc tagcatcgcg acgattgata
     5101 aaaatcgagg gaagttagtc ttccgcaatg tccattatac gaagagaggg cgaaatgtta
     5161 caactcagtt tcaaatcgat aacaggggaa ctgccggacg gaaatccagc atctttctga
     5221 caaaactgaa acgacactca ctataatgaa at
//
LOCUS       DASTMK010000490         4716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_376836, whole genome shotgun sequence.
ACCESSION   DASTMK010000490 DASTMK010000000
VERSION     DASTMK010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376836"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..2225)
                     /locus_tag="VFQ79_20490"
     CDS             complement(93..2225)
                     /locus_tag="VFQ79_20490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0142113.1"
                     /translation="MKLRVAFGTILAAALCAQTTSPPKPAQPKVTAAQPPYKALKFPP
                     LKRVEIPEIASHTLPNGIRLYLLENHELPLVSGFALIRTGNLFDPPEKIGVAEITGEV
                     LRTGGTKAKSGDELDVELENMAASVESSIGETSGTVGFSALRENAPRVLEIFKEVLTD
                     PAFRQDKIDLVKTQLRGVIARRNDNPSGIASREFARVLYGPDTPYGEIIEYENLDRIA
                     REDLVSFYNRYYFPANAVIAIQGDFSSAEMKATIEKLFADWTVKQPPVPPFPKVEQKP
                     TPGIFLGEKNDVNQTFFSVGHLGGILKDKDYPALAVMADILGGGFSSRLFTRVRTQLG
                     YAYGIGASWGAAYNHPGLFRISGSTKSASTVDTLKVVREEVDRIRTREVTAQELDTAK
                     QTILNSFVFNFDSPSKTLNRLVTYEYHGYPRDFIFQYQKAIESVTSADVLRVAREHIA
                     PENFTVVAVANSAELGTPLTALGNVQPIDLSIPEPGAKAGAKADAQSIEKGRQLLQRA
                     QAAMGGADKLAAIRDVTQVAEVEINTGRGPMKATRTSMWLAPNVFRQQQELPFGKITA
                     FYDGTAGWIASPQGINPIAGPVLNQVRSEMFRVFYPLMLSDRNPDRTVHSAAPGEVEI
                     SDKQGNSVRLTIDETTGLPVKRFYRTTGQQGPVEVEEVLSEWKAVDGVQLPYRIVVNQ
                     GGKKFSEASVREIQTNTGLKQEELSKQP"
     gene            complement(2222..3739)
                     /locus_tag="VFQ79_20495"
     CDS             complement(2222..3739)
                     /locus_tag="VFQ79_20495"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0142114.1"
                     /translation="MTTQRRGSLFGILAVFLPLILSAQSLQDFEKRVTEFTLPNGMHF
                     IVLERHEAPVVSFHAYANVGSVDDPSGKTGLAHMFEHMIGKGTQTVGTENWPAEKKAL
                     EAVEEVYDRLEAERRKELEANPETIKKLEAQLQAAIEKANSYVVPNLFVRVIEENGAA
                     GFNASTGSDATRYYYSLPSNRIELWFLLQSEWFKRPVFREFYKERSVVREERRMRVES
                     NPQGKLQELLTATTFVAHPYRTMIGWASDIENLRAKDAEEFFKTYYVPGNVTIAIAGD
                     VNPAQVKQFAQKYFGPIPGGELPPRVITEEPPQEGTKTVTLETAIQPFVFAAYKRPDQ
                     LHKDDPVFDVLGGILSSGRTGILYKELVRDKQLALGAVAIASFPGGKYLNAFLFIAAP
                     NPGKTIEEIQKAWQDILHRLKEETVDAETLERVKTKIRASLIRQLDSNPGLASQLAFY
                     HVNYGDWRKLFTGIQEIEKVTAADVQRVTREYLNDKTATLAYTTALKPASKGEGK"
     gene            3846..4628
                     /locus_tag="VFQ79_20500"
     CDS             3846..4628
                     /locus_tag="VFQ79_20500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142115.1"
                     /translation="MIAMLWAVGLLAGTSMAAEQAQRQTVPEPAAEKDVIYRSKAEGL
                     KATEPAPKPVPALTQRQLGDIMMARKRYREAIDIYTGETGRLPLVLTKLGIAYHQSGQ
                     RDQAKESYQNALEWDGRYAAAINNLGVLELDNRNARSAERRFQTALSLAAKSPAVQWN
                     LAAALFARERYQDAIAAFTTAVQLEPGIEKRAGYDMEEPMLIASVERTPEFDFCLYSA
                     YERAGMKEVAAEHLEKARSSGFEKETLKNSNGFIEWFLGLFH"
BASE COUNT          908 a         1345 c         1421 g         1042 t
ORIGIN      
        1 ggcgatagcc tcatccacaa gccgcttgcc gcgctcctgc gttgtctcgg cggaaagcag
       61 agacagactc atcacaaaca gcagcagaac cctcatggtt gtttgctcaa ttcctcctgt
      121 ttcaatccgg tgttggtctg aatttcgcgg acggacgctt cggagaactt cttgccaccc
      181 tgatttacga caatcctgta gggcagttgc acaccgtcta ctgctttcca ctcggagagt
      241 acctcttcca cttcaacggg tccctgctgc cctgtcgtac gatagaagcg tttgacgggt
      301 aatccggtag tttcgtcgat ggtgagacgg acggagttgc cttgcttgtc ggagatctct
      361 acttcgccgg gggcggcaga gtgcaccgtc cggtccgggt tccggtcgct gagcatcagc
      421 ggatagaaca cccggaacat ttcgcttcgt acctggttca gaacggggcc ggcgatggga
      481 ttgatcccct gggggctggc gatccaaccg gcggtgccat cgtagaatgc ggtgatcttg
      541 ccgaatggaa gctcctgctg ttgccggaac acattcggag caagccacat tgaagtccgg
      601 gtggccttca tcggtccgcg gccggtgttg atttcaacct ccgctacctg tgtcacatca
      661 cgaatagcgg ctaacttgtc cgcgccaccc atagccgcct gcgcccgctg aagcagttgc
      721 cgccctttct cgatgctttg cgcgtccgct ttcgcgcctg ctttcgcccc gggctcggga
      781 atggagaggt cgatcggctg cacgttgccg agagccgtaa gcggcgttcc caactcggcg
      841 gaattcgcga cggcgacaac cgtaaagttc tccggggcga tatgctcgcg cgccacgcgc
      901 agcacatcgg cgctggttac ggattcgatc gccttctggt actggaagat gaagtcacgc
      961 ggatagccgt gatattcgta agtgaccagg cggttgagcg ttttgctggg gctgtcaaag
     1021 ttgaacacga agctgttgag aatggtttgc ttcgcggtgt ccagctcctg ggcggtcacc
     1081 tcccgcgttc gtatgcggtc cacttcctcc cgcaccacct tcaaggtatc cacggtagag
     1141 gcggatttgg tgctgccgga gatgcggaac agaccagggt ggttatacgc cgcgccccaa
     1201 ctcgcgccga ttccgtaggc atagcccaac tgagtgcgga cgcgagtgaa cagccggctg
     1261 gaaaaaccac cgcccagaat gtcggccatc acggcgagtg cgggatagtc cttgtccttc
     1321 aggattccgc cgaggtgccc tacgctgaag aaggtctggt tcacgtcgtt cttctccccg
     1381 agaaatatcc caggcgtggg tttctgttcc actttcggga agggtggaac cgggggttgc
     1441 ttcaccgtcc aatccgcgaa cagcttttcg atggtcgctt tcatctcggc ggatgagaag
     1501 tcgccctgaa tcgcgatgac ggcattggct ggaaagtagt aacggttgta aaaagaaaca
     1561 aggtcctcgc gggcgatccg atcgaggttc tcgtattcga tgatttcgcc atacggcgta
     1621 tccggaccgt agaggacacg cgcgaactcg cgggaggcga tgcccgatgg gttatcgtta
     1681 cgccgggcga tgacaccgcg cagttgcgtt ttcaccagat cgatcttgtc ctggcggaag
     1741 gcgggatctg tcaggacctc cttgaagatc tcgagcaccc tgggagcatt ctcgcgcagc
     1801 gcgctgaagc ctaccgtgcc gctggtctca ccgatgctgc tctcgaccga cgcggccatg
     1861 ttctcaagct ccacgtcgag ttcatcgccg ctcttcgctt tcgtaccacc ggttcgcagg
     1921 acctcgcctg taatctcggc gacgccgatt ttctccggcg gatcgaaaag gttaccggtc
     1981 cggatcaggg cgaatccgct caccagcggg agctcgtgat tttccagcag gtagagccga
     2041 atgccgttcg gcagcgtatg tgaggcgatc tccggaatct cgacacgctt cagcggaggg
     2101 aacttcagcg ccttgtacgg cggctgcgcg gcagtaactt tcggttgggc cggtttgggc
     2161 ggtgaggtgg tctgcgcgca aagagcggca gcgagaatgg ttccgaacgc gacgcgcagt
     2221 ttcactttcc ttctcctttg gacgctggtt tcagtgccgt ggtgtacgcc aaagttgccg
     2281 ttttgtcatt caggtattcc cgcgtgacac gctgaacatc ggcggcggtt actttctcga
     2341 tctcctggat cccggtgaag agttttcgcc agtcgccata gttcacgtgg tagaacgcga
     2401 gttgcgaagc cagaccagga ttactgtcga gctgccggat cagcgatgca cggattttcg
     2461 tctttacgcg ttccagcgtc tccgcgtcca ccgtttcctc tttgaggcgg tgcaggatgt
     2521 cctgccacgc tttctgaatc tcctcgatgg ttttgcctgg attaggagcc gcgatgaaca
     2581 gaaatgcgtt gagatacttc ccgcccggaa agctggcgat cgctaccgcg cccagggcca
     2641 gttgcttgtc ccgcaccagt tccttgtaga gtatcccggt acgtccactg gaaaggatgc
     2701 ctcccagaac atcgaacacc ggatcgtctt tatgcaattg atccggcctt ttgtaagcgg
     2761 cgaagacgaa tggctggatg gcagtctcga gcgttaccgt tttcgtacct tcctggggag
     2821 gctcctcggt aatcacgcgc ggcggaagct cgccaccggg aatgggcccg aaatatttct
     2881 gcgcgaattg ctttacctga gccgggttca cgtcccctgc aatcgcgatc gtcacgttgc
     2941 cggggacata gtaggtcttg aaaaattctt ccgcatcttt cgcccgcagg ttttcgatat
     3001 cgctggccca gccgatcata gttctatagg ggtgggcgac aaaggtagtt gcggtcagca
     3061 actcctgaag cttgccctga gggttcgatt ccacgcgcat gcggcgttct tcgcgcacca
     3121 cggaccgctc cttgtaaaat tcgcggaata ccgggcgctt gaaccactcc gattgcagaa
     3181 ggaaccaaag ctcgatgcga tttgacggca ggctgtagta gtaacgggtg gcgtcagaac
     3241 cggtgctggc gttaaagccg gccgctccgt tctcctcgat gacgcgcacg aacaggttgg
     3301 gaacgacgta gctgttcgct ttctcgatag ccgcctggag ctgtgcttcc agcttcttga
     3361 tcgtctcggg attggcctcc agttctttgc ggcgttcggc ctccaggcgg tcatagacct
     3421 cctcgacggc ttccagcgct ttcttttcgg cgggccagtt ttcggtgccc acggtctggg
     3481 tgcccttgcc gatcatgtgt tcaaacatgt gggccaggcc ggtcttcccg ctggggtcgt
     3541 cgacggagcc gacattcgcg taagcgtgaa aagagaccac gggcgcttcg tgccgttcga
     3601 gcacgataaa gtgcattccg ttcggcagcg tgaactcggt cacccgtttc tcaaaatcct
     3661 gtagactttg agcggaaagg atcaagggaa gaaaaacggc gaggatcccg aaaagagacc
     3721 ccctgcgttg ggttgtcatg cagttttctc cagaactgaa gcaaacagaa ccattatctc
     3781 ccaggatttg agacacgcca ggggaagttt ggctgttgtc taatggtgtt aggaaggcaa
     3841 acgccatgat tgcgatgttg tgggcggtgg gtctgctggc cggcacgtcg atggcggcgg
     3901 agcaggccca gcggcagacc gtaccggagc ctgcggcgga gaaagacgtc atttaccgct
     3961 ctaaagcgga ggggttgaag gccaccgagc ctgcccccaa gcctgtgcca gccctgacgc
     4021 aacggcagct cggcgacatt atgatggcgc ggaaacgcta ccgcgaagcc atcgacattt
     4081 acaccggtga aaccggacgc ctgcccctgg tgctgacaaa gctggggatt gcctatcacc
     4141 agtccggaca gcgggaccag gcgaaagaaa gttatcagaa cgcgctcgaa tgggacggcc
     4201 gttatgcggc tgccatcaat aacctgggcg ttctggaact ggacaaccgc aatgcgcgta
     4261 gtgccgaaag gcggttccag actgctctaa gcctggctgc gaaatcgccg gcagtgcagt
     4321 ggaacctggc ggctgctctg tttgccaggg agagatacca ggatgcaatc gccgcgttca
     4381 ccactgccgt tcagcttgaa cccggcattg agaaacgtgc aggctacgac atggaagagc
     4441 cgatgctcat cgcctcggtc gagcggacgc ccgagttcga tttttgtctg tacagcgcgt
     4501 acgagcgggc gggcatgaaa gaggttgcgg ccgagcatct ggaaaaagcg cgcagttcag
     4561 gatttgaaaa ggaaacgctc aaaaacagca acgggtttat cgagtggttt ctgggtctgt
     4621 tccactgatc tgccgcgcca gctcaaaccg cactgccggc ctgccgctcc ggatcgagca
     4681 gggtctgaag ccgttccgcc gggaggccgc tttttt
//
LOCUS       DASTMK010000491         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_440932, whole genome shotgun sequence.
ACCESSION   DASTMK010000491 DASTMK010000000
VERSION     DASTMK010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440932"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFQ79_20505"
     CDS             complement(<1..803)
                     /locus_tag="VFQ79_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU0142116.1"
                     /translation="MMENKNSFGAAAPFRSGDRDYRIFRLDALEKRGVTNLSRIPYSI
                     KILLENLLRNEDGVAVKASDIEYVAGWDGKMPSKEISFRPARIVLQDFTGVPAVVDIA
                     AMRDALSRMGADPRKANPLIPADLVIDHSVQVDQFGSSNAFAFNTMLEFQRNHERYAL
                     LRWAQTAFTNFSVVPPGTGIVHQVNLEYLAPVVFRRDDLAYPDTLLGTDSHTTMINGL
                     GVVGWGVGGIEAEACMLGQPISMLLPKVMGYKLKGQLRPGATATDLVLT"
     gene            complement(873..1142)
                     /locus_tag="VFQ79_20510"
     CDS             complement(873..1142)
                     /locus_tag="VFQ79_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU0142117.1"
                     /translation="MCRNIRTLFNFDPPVTEEEIRAAALQFVRKISGFNKPSKANEAP
                     FLAAVDEIAGISTRLLHSLETHAAPKNREETAARAKARYAKRSGA"
     gene            complement(1156..1380)
                     /locus_tag="VFQ79_20515"
     CDS             complement(1156..1380)
                     /locus_tag="VFQ79_20515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0142118.1"
                     /translation="MPIYEYRCQECGNKFEKLVRRPGNGDITCPSCGNAQVKQELSTF
                     AAHSGGKQSASLPMCPSGGTCPTPGACGLN"
     gene            1466..2806
                     /locus_tag="VFQ79_20520"
     CDS             1466..2806
                     /locus_tag="VFQ79_20520"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEU0142119.1"
                     /translation="MIPLFLLAAVTLHPQSARKRKSPPPAPPAASVPTEWPIETLKVT
                     GNEVFEPAQILAVAGLKIGERAGKARFEAARDRLLATGAFESVGYRFEPGGSGKGYNA
                     TFEVVEVQPMYPVRFEDLPIPAAEIQKALEQAEPLFASRIPATDKMLQRYARRIEQYL
                     SSRGMAEEVAGRIVSDEPGKLEIVFRPAKAPPTVAEVRFTGNSVIPTTTLQNTMAGVA
                     VGTVYRETRFRQLLDANLRPLYEARGRVDVSFPKISTMPAQDVNGLVVTVGVDEGNTY
                     ELGTVKIDAPEEIPADDLLKAGAFKIGDLADFDQIEQGRKRIVDALKRQGHMLSAATI
                     ERSVDHEKKVVDLVIHVTPGPRYVFGNLAIKGLDLLTEPAIRKLWGMKPGQPFNADYP
                     AYFLGRIREDGVLDNLRSTRPEIRVDEARKTVDVTLYFNEPPPSVLPGVPVPQR"
     gene            2809..3345
                     /locus_tag="VFQ79_20525"
     CDS             2809..3345
                     /locus_tag="VFQ79_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEU0142120.1"
                     /translation="MERLPYQVTASAAAYDRISTLPVAVLLDNVRSMYNVGAFFRTAD
                     AASIRKLYLSGITAAPPKPAITKTALGAEASVQWEYAWEPFRIIDRLIESEWEIAAVE
                     TGLHAVDLFDWTPRFPVCVVFGHEVDGLRPEVLDRCHTLVRIPMLGVKHSLNVATAGG
                     VVIFELLRKYRRIVESAG"
     gene            complement(3409..>3664)
                     /locus_tag="VFQ79_20530"
     CDS             complement(3409..>3664)
                     /locus_tag="VFQ79_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142121.1"
                     /translation="FIFLIRVNPCPSVANQIVAQTFRMSCLMRQLKKSKRPAVRLLDT
                     AAQTDAGEGIRQALEDMRKGRVRPAKEFFAEFETRHGLSY"
BASE COUNT          738 a         1077 c         1095 g          754 t
ORIGIN      
        1 atggtcagca ccaggtccgt ggccgtggcg cccgggcgga gctgcccctt cagcttgtaa
       61 cccattacct tcggcaacag catggagatg ggctgcccaa gcatacacgc ttcggcttcg
      121 atgccgccga cgccccatcc aaccactccc aggccattaa tcatcgtggt gtgggaatcc
      181 gtgccgagga gggtgtcggg gtaagcgaga tcgtcccggc ggaaaaccac cggcgcgagg
      241 tactcgagat tgacctggtg cacgatgccc gtacccggag gcacaacgga gaaattcgtg
      301 aatgcggtct gcgcccagcg aagcaacgca taccgctcgt ggttgcgctg aaactccagc
      361 atcgtattaa atgcgaatgc gttgctggat ccgaattgat ccacctggac ggagtgatcg
      421 ataaccagat cggcgggaat gagcggattc gccttccggg ggtcggctcc catcctgctg
      481 agcgcgtccc gcatcgctgc gatatccaca accgccggaa cgccggtaaa atcctggagg
      541 acaatacgag ccggccggaa gctgatctct ttggagggca ttttaccgtc ccagcccgct
      601 acgtattcga tgtcggaagc tttcacggcg acgccgtctt cattccggag cagattttcg
      661 agaaggattt taatcgaata aggaatgcgc gagagattcg tcacccccct cttttcaaga
      721 gcatccaacc ggaatatgcg gtagtcgcga tcgcctgagc ggaatggcgc agccgcgccg
      781 aagctgttct tgttttccat catgtgtttc tcagatctta tcctaatggt atcgccggaa
      841 acgcgcaggg catcgcagct tgcccgttcg tctcaagcgc cggatctctt cgcgtatcgg
      901 gctttcgccc gcgcggccgt ttcttcgcgg ttcttcgggg ccgcgtgcgt ttcaagcgaa
      961 tggagaaggc gggtggagat gcccgctatc tcatccacgg cggccaggaa tggggcttcg
     1021 ttcgctttcg acggcttatt gaatccgctg atcttcctta cgaactgaag cgcagccgcc
     1081 cgaatctctt cttccgtgac gggcggatcg aagttgaaga gcgtcctgat gttcctgcac
     1141 attgcgtggc ttgcgtcaat tcagaccgca ggcgccaggc gttgggcaag ttcccccgga
     1201 cggacacatc ggaaggctcg cggattgttt tccaccggaa tgcgcggcaa acgttgacaa
     1261 ctcctgcttt acctgagcgt tgccgcacga ggggcatgtg atgtcaccgt ttcccggacg
     1321 gcggaccagc ttctcaaact tgtttccgca ctcctggcag cgatactcgt agattggcat
     1381 agaatatcta taattttatc aacttctgat gaaacgcaac cgcaagatcc acgggacgtc
     1441 caactgaaat atggctccgc accggttgat ccctctgttt ctgctcgccg ccgtcacgct
     1501 tcacccgcag agcgcacgca agcggaaatc gccgccaccc gcaccgccgg cagccagtgt
     1561 tcccaccgaa tggccgatcg aaacactgaa ggtcacgggc aacgaagtgt ttgagccggc
     1621 acagatcctg gcggtggccg gcctcaagat cggggaaagg gcagggaagg cgcggtttga
     1681 ggcggcgcgg gacaggcttc tcgccacggg cgcatttgag tccgtcggat accgcttcga
     1741 gcccggcggc agcgggaagg gctataacgc gacatttgaa gtggtggagg tgcagcccat
     1801 gtacccggtg cgttttgagg atctccccat tcccgcggct gagattcaga aggcgctgga
     1861 gcaggccgag ccgctgttcg caagccggat acccgccacg gacaagatgt tgcaacgcta
     1921 cgcgcgcagg atcgagcagt atctttcaag cagagggatg gccgaagaag tcgccggccg
     1981 gattgtgtcc gatgagcccg gcaaattgga gatcgtattc cgccccgcga aggcaccgcc
     2041 aacggtcgcc gaggtacgct ttacgggtaa tagcgtcatt cccacgacga cgttgcagaa
     2101 tacgatggcc ggcgttgctg tcggaacggt ctatcgggag acccggtttc gacaactgct
     2161 cgatgcgaac ctacggcccc tctatgaggc gcgtggacgc gtggacgtct ctttcccgaa
     2221 gatctcgacg atgcccgcgc aggatgtcaa cgggctggtt gtcacggttg gtgttgacga
     2281 gggcaacacc tacgagttgg gcacggtgaa gatcgacgcc cctgaagaga tcccggcgga
     2341 cgatctgctg aaagcgggcg cgttcaagat aggtgacctg gctgatttcg accagatcga
     2401 gcagggacga aagcgcattg tcgatgccct gaagcggcaa gggcacatgc tttctgcggc
     2461 caccattgag cgtagtgtcg atcacgagaa gaaagtcgtc gatctcgtca tccatgtcac
     2521 tcccggtccc cgttacgttt tcggcaacct ggcgattaag ggattggatc ttctcaccga
     2581 acccgccatc cgaaaactct ggggcatgaa accgggccag ccgttcaacg ccgattaccc
     2641 ggcttatttt ctgggacgaa tccgcgaaga cggcgttctc gacaacttga gaagtacgcg
     2701 gccggagatc agggtcgacg aagcgagaaa gacagtggac gtgacactgt acttcaacga
     2761 gcctccccct tctgtgcttc cgggcgtgcc ggttccccaa cgttgaagat ggaacggctt
     2821 ccctatcagg tcacggcgtc tgccgccgcg tatgacagaa tttccacact gccggttgcg
     2881 gtgctgctcg acaatgtccg gagcatgtac aacgtcggag ctttcttccg caccgccgat
     2941 gcggcctcga tccgcaagct atacctttcc ggcattacgg cggcgccgcc gaagcccgcg
     3001 atcaccaaga cggctcttgg cgccgaagcg tccgtccaat gggagtatgc gtgggaaccc
     3061 ttccggatca tcgaccggct tatcgaaagc gaatgggaga tcgccgcggt cgagaccggc
     3121 ttgcatgcag tcgatctgtt cgactggacg ccccgctttc ccgtctgcgt ggtcttcggt
     3181 cacgaggtag acgggctacg cccggaagtg ctggatcgct gccacaccct ggttcgcatc
     3241 cccatgctcg gcgtcaagca ctctttgaac gttgcgacgg ctggaggcgt ggtgatcttc
     3301 gagcttctac ggaagtaccg ccggattgtt gaatccgctg gataaaacgg tgttggatgt
     3361 gaggccggcc cttgcggctg gtgggacata caggcgcaga ccgttaggtt aataagatag
     3421 gccatgccgc gtttcgaatt ccgcgaagaa ttcctttgcc ggccggaccc taccctttct
     3481 catgtcctcc agtgcctggc ggatgccctc cccggcgtct gtctgagcgg cggtatccag
     3541 cagccgcacc gcgggacgct tgctcttctt gagctgcctc attaaacacg acatcctaaa
     3601 tgtttgcgcg acgatttgat tggccacgga tggacacgga ttcacacgga taagaaaaat
     3661 gaag
//
LOCUS       DASTMK010000492         1949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_313555, whole genome shotgun sequence.
ACCESSION   DASTMK010000492 DASTMK010000000
VERSION     DASTMK010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313555"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1949
                     /locus_tag="VFQ79_20535"
     CDS             <1..>1949
                     /locus_tag="VFQ79_20535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142122.1"
                     /translation="RYGRAAEARSCFEGLTRSQDAYARAEGFWGLERFEEANDSFRAA
                     VKASPGNAHYQVRWGQLYLERVQPADAIQLFTEALKLEPDNPRALLGIAEAAASNFDR
                     RALELAQKALAVDPKLYQAHELLARVALEDGDQAKAREEADKALAISPEALDAMAVRA
                     TIDWMNEQPSTEWMERVLKVNPIYGEAYALAAHLFVINRRYEEGIAFYRKAIELNPRL
                     WPARSQLGVNLMRLGREKEARQQLEMCYENGYKDPATVNTLRLMDSYKNFKTFSGGNI
                     SIRLHNREADLLFPYFEAELRRAIATYDRKYKMKLDHPVQIEVYPDHEDFAVRTVGMP
                     GLGALGVTFGHVIAMDSPSGRKPGTFHWASTLWHELSHVYVLAATKHRAPRWFTEGMA
                     VHEETATSPDWGDRLDPDIIDAIRKKRLLPVAELDRGFIRPSYPNQVIVSYFQAGRIC
                     DFINEKWGYDKLLGMMHAFTGSKTTPQVIEEQLGVKASEFDGQFLQWLDAQVKKTVDG
                     FDDWRKQMKEIAANAKKNDHDEVIRQGRAIRDIYPDYVEAGSVYEFLADAYLAKNDKT
                     SAMGELETYARTGGRDPALLKKLATLQEEAGRKKEAAETLNRLNYIYPVNDEELHQRL
                     GGLYLETGNLDSAIREYTAVVAMTP"
BASE COUNT          451 a          538 c          615 g          345 t
ORIGIN      
        1 ccgctatggc cgggcagccg aagcccgatc ctgtttcgag ggactgactc gttcgcaaga
       61 cgcttacgcg cgggccgaag ggttctgggg ccttgagcgg ttcgaggaag cgaacgattc
      121 ctttcgagct gccgtcaaag cttcgccggg caacgcgcat taccaggtgc gatggggtca
      181 actctatttg gagagagtcc aaccggcgga tgccattcag ttgttcaccg aggcgctgaa
      241 gctggagcca gacaatccgc gggcgctgct gggaatcgcg gaagcagcgg cgtcgaactt
      301 tgacaggagg gctttggagc ttgcgcagaa agcgttggcg gtagatccga agctttacca
      361 ggctcacgaa ttgctggcgc gagtcgcgct cgaagacggg gaccaggcaa aggcgaggga
      421 ggaagcggac aaggcgctgg caatttcacc ggaagctctc gatgcgatgg cggtccgggc
      481 aaccatcgac tggatgaatg aacagccatc cacggagtgg atggagcgtg tcctcaaggt
      541 caaccccata tacggggaag cctatgcgct cgccgcacac ctgttcgtta ttaatcgccg
      601 ctatgaagaa ggcatcgcgt tttacaggaa ggcgatcgag ttgaatccgc gcctgtggcc
      661 ggcgcgctcg caactgggcg tgaatctgat gcgcctcggc cgggaaaaag aagcgcgcca
      721 gcagcttgag atgtgctacg agaacgggta caaagacccc gctaccgtca acacgcttcg
      781 gctgatggac agctacaaga atttcaaaac cttcagcggc ggcaatatct ccatccggct
      841 gcacaatcgc gaagcggacc tgctgtttcc ctacttcgag gcggaactcc ggcgcgccat
      901 cgccacatac gaccgcaaat acaaaatgaa gctcgatcat cccgtgcaga tcgaggtcta
      961 tcccgatcat gaggactttg cggtgcgaac ggtgggtatg ccgggactgg gagctcttgg
     1021 tgtcaccttc ggccatgtga tcgcgatgga cagcccctcc gggcgaaagc cgggaacctt
     1081 tcattgggca agcactctgt ggcatgagtt aagccacgtc tacgtgctgg ccgcgacaaa
     1141 gcaccgggcg cccagatggt tcaccgaagg catggccgtg cacgaggaga ctgccacgtc
     1201 gccggattgg ggcgaccgcc tggaccccga cattatcgat gcgatccgga aaaagcgatt
     1261 gctgccggtg gccgagctcg accgaggatt catccgcccc agctatccga accaggtgat
     1321 cgtttcctac tttcaggccg gccgcatttg cgatttcatc aatgagaaat ggggctacga
     1381 caagctactc ggaatgatgc acgcgttcac cggctcgaag acgacgccgc aggtgattga
     1441 ggaacagctt ggtgtgaaag cgtccgaatt cgacgggcag tttctgcagt ggctggatgc
     1501 acaggtgaag aagaccgtag atggattcga cgactggcgc aagcagatga aagagatcgc
     1561 ggcgaatgcg aaaaagaacg atcacgacga agtgattcgc caggggcgcg ccatccgcga
     1621 tatttatccc gactacgtgg aagcgggcag cgtctatgaa tttttagccg acgcttacct
     1681 ggcgaagaac gataaaacca gcgccatggg ggaactcgaa acctacgcgc gcaccggcgg
     1741 ccgcgaccct gccctgttga agaagctggc tacactccag gaggaagccg gcaggaagaa
     1801 ggaagccgcg gagacgctga accggttgaa ttacatctac ccggtcaacg acgaggaact
     1861 gcatcagcgg ctcggcgggc tgtatctgga aaccggcaac ctggatagcg caattcgcga
     1921 gtatacggcc gtagtggcga tgacgccgc
//
LOCUS       DASTMK010000493         8113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_121050, whole genome shotgun sequence.
ACCESSION   DASTMK010000493 DASTMK010000000
VERSION     DASTMK010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121050"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFQ79_20540"
     CDS             <1..670
                     /locus_tag="VFQ79_20540"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU0142123.1"
                     /translation="LRTFLPKGWEEMARKSGALRRARDIPDAESLLRVLLIHVANGYS
                     LVDSEILGLQWGRVDLKRLFLTVGDSKTEAGEGRTVPINDELLTVLNEHVKWYEKKFK
                     ARRPEWFLFPHGKPQPTDPTRPMTTLKTAWKKVKDDAGVTGRFHDTRHTFITDLAESG
                     EAGDETIRDLAGHVSKQMLKHYSHIRMEAKRRAVGALSKKKSEKVVSISDGPLQEIPK
                     VAGF"
     gene            1032..1373
                     /locus_tag="VFQ79_20545"
     CDS             1032..1373
                     /locus_tag="VFQ79_20545"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEU0142124.1"
                     /translation="MPDSAPDMRLATYGTLAPGRVNHHHLAGLSGRWQRGAVRGRLVD
                     AGWGSALGFPALVLDPMGPLVDVYLFESSDLPDHWFRLDEFEGPGYTRVVTQVSTADG
                     ELGAWIYVVAR"
     gene            complement(1566..1688)
                     /locus_tag="VFQ79_20550"
     CDS             complement(1566..1688)
                     /locus_tag="VFQ79_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142125.1"
                     /translation="MASQNTDYILLDQERIGWMFPFATAAFSLIRMRNLLAATA"
     gene            complement(1695..2624)
                     /locus_tag="VFQ79_20555"
     CDS             complement(1695..2624)
                     /locus_tag="VFQ79_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0142126.1"
                     /translation="MVRDELSNLAAFLAVAEERSFTRAAKRLGVTPSAMSHAMRELEE
                     AMGVRLLSRTTRSVAPSQAGEQLLARLRPALGDITEAMEQISGLRNKPAGLVRLLVPR
                     LAASTVLGPKLMQFARDYPDIVLDIAADDSRMDIVAGGFDAGIHYGEYIQKDMIAVRV
                     SHDHRAAIVGSPGYFDSHPKPKSPHDLLNQRCISFRHGTAGIYRWEFDKGKKALSVSV
                     TGPLIVDDVEIMTRAAIDGIGLAFMSEEHAAPYLENGSLLRVLEDWCQPFPGFFLYYP
                     SRRQQPAALSALIDTLRLRSDKKHQSNESRFLR"
     gene            2724..3875
                     /locus_tag="VFQ79_20560"
     CDS             2724..3875
                     /locus_tag="VFQ79_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0142127.1"
                     /translation="MPSNNGRESAEGESGAARRDFLKLAGTAAGMAAASWAIPAAAQT
                     REQKGNNTIITIRKLGTLAVSQLGFGNMGLSGGHYGPGVDRALGIRVIRNAHERGVTF
                     FDTAEVYGPYVNEELVGEALAPVRDKVVIATKFGFKIDGTNGLDSRPERIRKVVEESL
                     KRLRTDRIDLYYQHRVDLTVPIEDVAGTIKDLIQAGKVLHFGLSEPSAKTIRRAHAVQ
                     PITAIQTEYSLIERSPERNGVLQACEELGIGFVPWGPLGQGFLPGKMDVNIQPTFDPK
                     NDLRATFPRFSPIVMKNNRPIIELLTAFGAKRGATPAQIALAWLMAQKPWIVPIPGTG
                     NMDHLSENIGAVNAQLTPADLRELETAFSRIQIYGGRMDARQMAQIGQD"
     gene            4155..4496
                     /locus_tag="VFQ79_20565"
     CDS             4155..4496
                     /locus_tag="VFQ79_20565"
                     /inference="COORDINATES: protein motif:HMM:NF037716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEU0142128.1"
                     /translation="MKVRIGSTTFDAALFDNETAAAFKALLPLTLDMDELNGNEKMYD
                     LSKPLPTSAYSPKRINSGDLMIWGNRTVVLFYKTFPTSYSYTRLGRIEDPSALEAAVG
                     SGRVKVTFALE"
     gene            4554..5342
                     /locus_tag="VFQ79_20570"
     CDS             4554..5342
                     /locus_tag="VFQ79_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0142129.1"
                     /translation="MRFKETKMENPLNFNGKVALVTGAAAGMGLATAQAFVEAGAAVV
                     LADVREDLVRAEAQKLVAAGHKAIAVRCDVSDDAQVAAMVDRTVAEFGRLDAAFNNAG
                     VMPRIAPTAESTIEEWERVIGINLRGVWSCMKYELRQMERQGSGAIVNNGSIGAFTGN
                     PGIGSYIASKHGVVGLTRTAALEYIRHGIRVNAVNPGTIDTQIARDVVGGDAQAFDKL
                     ASQPPIGRAGRPEEIASVVLWLCSPGASYVVGQALTVDGGLTVP"
     gene            5473..5787
                     /locus_tag="VFQ79_20575"
                     /pseudo
     CDS             5473..5787
                     /locus_tag="VFQ79_20575"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            complement(5866..6633)
                     /locus_tag="VFQ79_20580"
     CDS             complement(5866..6633)
                     /locus_tag="VFQ79_20580"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEU0142130.1"
                     /translation="MNPAHAVIGFRTLPWRHQADAVAFVRGLYGRGKRGAMIAAVMGT
                     GKSAMTVYLCVEEGFQIVLILCPLRVVQVWRPQFEMHSTVPFLVVALDDSFGNVRAKR
                     AEAERQIGLAKARGVPVVIVINYDSAWRSPFAEWALRQKWDLVVADEIHRCKAPGGKA
                     SRFLARLGKATRFRLGLSGTPMPHSPLDVYAYFRFIDPLVFGWSFHKFRQHYAVMGGY
                     QNHQIVAYSTVDEKVMAALAHRSDVVNTVLQQMKGGR"
     gene            complement(6630..7148)
                     /locus_tag="VFQ79_20585"
     CDS             complement(6630..7148)
                     /locus_tag="VFQ79_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142131.1"
                     /translation="MRTSRRSELTEAIRETVAAFCAAGRRFWNRDIVDTVIEQNGSLF
                     ADLGQQLAREKLFDLTRRVMKTAAEVTEAEARLQLGLDLPEFEMPNLIAVPVDIMNPL
                     NGDCEWVPVMKATVADLDSNLRLLDVQIAADQRRRRHIAMLRQRVVAVVGEKTELTVA
                     EAAALAREMQPA"
     gene            complement(7135..8007)
                     /locus_tag="VFQ79_20590"
     CDS             complement(7135..8007)
                     /locus_tag="VFQ79_20590"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEU0142132.1"
                     /translation="MGDVETLAASIATVGLLHPPVVTEDGRLICGERRFLAMRDILRW
                     KTIPVIILQVSSIVAGEYAENEIRKDFTPSERVAIAKALEAEIGDRQGQRTDKELPPN
                     PAEVVPGVETRDIAAKKAGFGGHTTYELAKKVVEKAVDEVVAQMDSGDLSISAASLIA
                     DQPVETQREIAQLPEGEKKAAVRKLRRKDLPTPAAARKQALTSGLAILDRNLNYQLPT
                     PESQRPIVERNYAAMAIADAARAISGCQHSAEEIAAGIRSLDTPDMDFAGHCRKAAAF
                     LNEINQELERNENK"
BASE COUNT         1639 a         2359 c         2545 g         1570 t
ORIGIN      
        1 ccttcggaca tttctgccca agggctggga ggaaatggct cgcaagagcg gagcactcag
       61 gcgagcccgt gatatcccgg acgcggagtc cttgctgcgt gtcttgctga tccacgtggc
      121 caatggatac tcgctggtcg attcggaaat cctggggctt cagtgggggc gggttgatct
      181 gaaacggctg tttctcaccg tgggggacag caagacggaa gccggggaag ggcggacggt
      241 cccaattaac gatgaactgc tgacggtgct caacgagcat gtgaagtggt acgagaagaa
      301 gttcaaggcg cggcgcccgg agtggttcct gttcccccac ggcaaaccgc aaccgaccga
      361 cccgacgcgg ccgatgacca cgctgaaaac cgcctggaag aaggtcaagg acgatgccgg
      421 ggtgacgggc cggttccacg atacgcgcca cacgttcatt accgatctgg cggaaagcgg
      481 ggaagccggg gacgaaacca tacgcgatct ggcgggccac gtctcgaaac agatgctcaa
      541 gcactattcg cacatccgca tggaagcgaa gcggcgggcc gtgggcgcgc tgtcgaagaa
      601 aaaatctgag aaggtggttt cgatttccga tggaccccta caagaaatcc ccaaagtggc
      661 cggtttttga gggggtggaa atgctcgtaa gttattggct ccccaagtca aacaaagggt
      721 attttgcggc ttagagacac tggcaaaatg attgatacgg aatgggttac tcaaacgccc
      781 tgcgccaccg tctccgtttc ggccgattcc gtgtggcggc caggttcgag caatgtcccg
      841 tccccggcca aattgcggtg aacaaagcca cgatgtagag tcgcacggct tttcgcgagg
      901 tgggtccagg cccagcttcc gagaatgaga catcctagga gattcggata ctctgcctgc
      961 tttgcaatca aatctgtcac cgccgtccat cgacggcgac cctcgacaac ctgggcaagg
     1021 gtgcaatgcc aatgcctgac agcgcgccag atatgcgttt agccacctac ggcactctcg
     1081 cgcccggacg agtcaatcac caccacctcg ccgggctgag cgggcgatgg caacgaggcg
     1141 ccgtgcgagg ccggcttgtg gatgctgggt ggggatctgc gctcggcttc cctgcgttgg
     1201 tcctcgaccc aatgggccca ctcgtggacg tgtatctttt cgagtcatcg gacctgcctg
     1261 atcattggtt ccgtctcgac gagtttgagg gacctggcta cacgcgagtt gtcactcagg
     1321 tgagcacggc agacggcgaa ttaggtgctt ggatctatgt agttgctcga tagatctgct
     1381 gcaatgcaaa tgcttgcggg cttgagttcg gcgattgtgc ctcgaagccg gcgctcccgt
     1441 ctacgatccc caaaaaaacg cgggcggtcg tccatcttgc aggaacgggc aggtctagtt
     1501 tgagtgaggg ttgaaggctt cggcacggcc cgggcgaggg tgcgcggggg cgcttcgggc
     1561 ggcgctcagg cggtcgccgc cagcaggttt cgcatgcgga tcaggctgaa ggcagccgtg
     1621 gcgaaaggga acatccagcc gatgcgctcc tggtcgagca aaatgtaatc cgtgttctga
     1681 ctggccatgc ccctttacct taagaagcgc gattcgttgc tctggtgctt tttgtcgctg
     1741 cgcaggcgta gggtgtcgat cagcgcagac agcgcggctg gctgttgcct gcgacttggg
     1801 tagtagagga agaaaccagg gaagggctgg caccagtctt cgagtacccg caggagtgat
     1861 ccgttttcaa gatatggcgc ggcgtgttct tccgacatga acgccaagcc gattccgtca
     1921 atcgctgcgc gggtcatgat ctccacatca tcgacaatca gtggtccagt gacggacacg
     1981 gacagtgctt tcttgccctt gtcgaactcc catctgtata ttccagccgt tccgtgtcga
     2041 aaactaatgc atctctggtt aaggaggtca tgaggcgatt tgggctttgg atgcgaatcg
     2101 aagtagccag gcgagcccac gatcgccgca cggtggtcgt gggagacgcg aacggcgatc
     2161 atgtccttct ggatgtattc cccgtaatgg attcccgcat cgaagccacc ggcaacaata
     2221 tccatgcggc tgtcgtccgc ggcgatgtcg agcacgatat ccgggtagtc acgcgcaaac
     2281 tgcatcagtt tcggtccgag cacggtcgat gccgccaggc gtggcaccag aagacgcacc
     2341 aggcccgccg gtttgtttcg aagcccggag atctgctcca ttgcttccgt aatgtcaccc
     2401 aaagccggcc gcagccgggc gagaagttgt tcgcctgcct gggacggcgc aacgctccgg
     2461 gttgttctgg agagcaggcg gactcccatg gcttcctcca attcccgcat tgcgtggctc
     2521 atcgctgaag gggtaacgcc caggcgcttt gctgcccgtg taaagctgcg ttcctccgcc
     2581 acggcgagga aggcggccag attgctcagt tcgtctcgca ccatttgtga attgtactca
     2641 caagcttata tggatttatg gggcttctca agtgttcgat ttggccttac cttggattca
     2701 gaggacaagg aggaatagcg aacatgccat ccaacaacgg gcgcgagagc gcggaaggag
     2761 aatccggcgc cgcccggcgc gatttcctga aactggccgg tacagccgcc gggatggcgg
     2821 cagcgtcgtg ggcgatccca gcggccgcgc aaacccgaga acagaaagga aataacacca
     2881 ttatcaccat ccgtaaactt ggcaccctgg cggtgtccca actcggcttt ggcaacatgg
     2941 gtctcagcgg cggccactac ggtccgggtg tggaccgggc cctgggcatc cgtgtgattc
     3001 gcaacgcgca cgaacgaggc gtcaccttct tcgacacggc ggaagtctat ggcccgtacg
     3061 tgaacgagga actggtcggc gaggccctcg cgccggtccg cgacaaggtc gtcatcgcca
     3121 ccaagttcgg cttcaagatc gacggcacga acggtttgga cagccggccg gagcgcatcc
     3181 ggaaggtcgt cgaggaatcg ctcaagcgcc tcaggaccga tcgcatcgat ctttactacc
     3241 aacaccgcgt cgatctcacc gtgccgatcg aagacgtggc cggcacaatc aaggatctga
     3301 tccaggccgg aaaggtcctg cacttcggcc tctcggagcc gagcgcgaag accattcgcc
     3361 gcgcacatgc ggttcagccg attaccgcca tccagaccga atactcgctc atcgaaagaa
     3421 gcccggaacg taacggcgtg cttcaagcct gtgaagagct tggaatcggc ttcgtcccgt
     3481 ggggaccact gggacaaggt ttcctgcccg ggaaaatgga tgtgaatata caacccacct
     3541 tcgacccgaa gaacgacctg cgagcgacgt tcccgcggtt ctcccccatc gtcatgaaga
     3601 acaataggcc gatcatcgag ttgctgacgg cgttcggcgc gaagaggggc gcaacccctg
     3661 cccagatcgc actcgcgtgg ctgatggcgc agaagccgtg gattgttccg atccccggca
     3721 ccggaaacat ggatcacctg agcgagaaca taggtgcggt caatgcccaa ctcacgccag
     3781 cggatcttcg cgaacttgag acggccttct cccgaatcca gatctacggt ggccggatgg
     3841 atgcgaggca gatggcgcag attggccagg actgacaaag cggccgatca gaattaagaa
     3901 ggaagacgat gaacggccat ctcaactgtc aaagtaaggt cgtcctggta agaggcgcca
     3961 cacgaacggg ccggaagatt tgttttcggg gttccgggct atgccggctg cctgcgatgg
     4021 cagggtttgc agttacggtg ctgtctcttt ccatcctggc tgtcgtggcc tccaataggg
     4081 ccgaagctgg gcaaccgcaa ggcggagatt cgagacgcga aggcaatgat gctaacactc
     4141 agagagggac caagatgaaa gtccgcatcg gttcaacaac gtttgacgca gcgctctttg
     4201 acaatgagac ggccgctgcg ttcaaggcct tactaccatt gacgctcgat atggacgagc
     4261 tgaacggcaa tgaaaagatg tatgatctct cgaagccgtt gccgaccagt gcttactctc
     4321 caaagagaat caatagtggt gatctcatga tttggggaaa tagaacggtg gtgcttttct
     4381 acaagacatt cccgacctcg tacagttaca caagactcgg gcgtatcgag gatccttcgg
     4441 cacttgaagc ggcagtaggt tcgggacgtg taaaggtcac cttcgcattg gaatgaggtt
     4501 gttgcgcatt tgacctgcga gagcccaggc actgccgcac ctgtgaaata gcgatgagat
     4561 ttaaggagac aaagatggag aacccattga acttcaacgg caaggtagca ctcgtcaccg
     4621 gggccgcagc gggcatggga ctagcaacgg cccaagcgtt cgtcgaggct ggcgctgccg
     4681 tcgttctggc cgacgtcaga gaagacttgg tgagggccga agcccagaag ctggtagccg
     4741 ccggccacaa ggcaatcgcg gttcgctgcg acgtgagtga cgacgcgcag gtggcagcga
     4801 tggtggaccg cactgtcgcc gagttcggcc ggctcgacgc tgcgttcaat aacgcgggcg
     4861 tcatgccccg gatcgcgcca accgccgaga gcaccattga agagtgggag cgcgtgatcg
     4921 ggatcaactt gcgtggcgta tggagttgca tgaaatacga actgcggcaa atggaacgcc
     4981 aaggcagcgg cgcgatcgtc aacaacgggt caatcggcgc gtttaccggc aaccccggca
     5041 ttggctcgta catcgcctcc aagcacggtg tggtgggcct gacgcggacg gcagctctgg
     5101 aatatatcag gcacggcatc cgcgtgaatg cggtcaatcc cggcacgatc gacacgcaga
     5161 tcgctcgcga cgtcgtcggc ggggacgcgc aggcgtttga caaactagcg agccagcctc
     5221 ccattggccg tgccggcagg cccgaggaga tcgcttcagt cgtattgtgg ctctgtagtc
     5281 cgggggcaag ctacgttgtg ggccaggccc tgaccgtgga cggcgggctg acggtgccgt
     5341 aacgcgagga gagatacccg actgtttgag cactcgagac gctcgtatag taaggcggcg
     5401 aagagcggcg cccgcgccga ggacgattcg acgatattcc acgcgaactt aacaatggca
     5461 aggagacaaa ccatgcgagc aactgttctt tacgggccac gtgacattcg gtttgaggat
     5521 cgtgagacac cgaagattat cgaacctacg gacgcggtgc tacgcatggc tgcgacgtgc
     5581 gtgtgcggat cggacctgtg gccgtaccgg ggcttgcagc cgatcgacgg ccccacggcg
     5641 atggggcatg agtactgtgg cttcgttgaa gaggtcggca gcgcagtcaa atctttgaag
     5701 ccgggacaat ttgtagtcgg ctcgttcgtg gcatccgaca acacatgccc taactgccgg
     5761 tacggctatc agtcgtcgtg catccaccgc gagttcatgt taagggcgca ggcaccgttc
     5821 ggtcacccat ttctgggtca gcagccggtc gatgaacctg ggcattcagc ggcccccttt
     5881 catctgctgg agaaccgtat tgacaacgtc ggagcggtgc gccagcgcgg ccataacctt
     5941 ctcgtcgacg gtgctgtagg cgacgatctg gtgattctgg tagccgccca tgacggcgta
     6001 gtgctgccgg aatttgtgga agctccagcc gaacaccaac gggtcgatga agcggaaata
     6061 ggcgtagaca tcaagcggcg agtgcggcat gggcgtgccg gacaggccaa gccggaaccg
     6121 cgtggccttg ccgagccgcg caagaaaccg gctggccttg ccgccgggcg ccttgcatcg
     6181 gtggatctca tctgcgacga caagatccca tttctgccgg agcgcccact ccgcgaaagg
     6241 cgagcgccaa gcgctgtcgt aattgataac gatcacgacc ggtacgccgc gagccttcgc
     6301 cagtccgatc tgccgttcgg cttccgcgcg cttggcgcgc acgttgccga aggagtcgtc
     6361 gagcgccacc acgaggaaag gtacggtgga gtgcatctca aactgcggcc gccacacctg
     6421 cacgacccgg agcggacaga ggatgagaac gatctggaaa ccttcctcaa cgcacaggta
     6481 gaccgtcatg gcggatttgc cggtgcccat gacggcggcg atcatggctc cgcgcttccc
     6541 acgcccgtag aggccgcgga cgaaagcgac ggcatccgcc tgatgccgcc acgggagggt
     6601 tcgaaatccg atgactgcgt gagcagggtt catgcgggct gcatctcccg cgccagtgcc
     6661 gccgcttccg caacggttag ttccgtcttc tctccgacga ccgccaccac gcgctgccgc
     6721 aacatggcga tgtggcggcg gcgccgctga tcggcggcga tctggacgtc caacaggcgc
     6781 aggttggagt ccagatccgc gacggtcgct ttcatcaccg gcacccactc gcagtcgcca
     6841 ttgagagggt tcatgatgtc cacgggcacg gcgatcaggt tgggcatctc gaattcgggg
     6901 aggtccagcc cgagctgcag gcgcgcctcg gcctcggtga cttcggccgc cgttttcatg
     6961 acccgtcggg taaggtcgaa gagtttctcc cgcgcaagct gctggcccag gtcggcgaac
     7021 agactgccat tttgttcgat caccgtgtca acgatgtcgc ggttccagaa gcgccggccc
     7081 gcggcgcaaa atgccgccac cgtttcccgg atcgcctccg tcagttcgga tcgcctactt
     7141 gttctcattg cgttcaagct cctggttgat ttcattgagg aaggcggccg cttttcggca
     7201 gtgccctgca aagtccatgt ccggggtgtc caagctacga atcccggcgg cgatttcctc
     7261 cgcggagtgc tggcagccgg agattgcccg cgccgcatcc gcaattgcca tggcggcgta
     7321 gttgcgttct acgatcggcc gctgcgactc cggcgtgggt agctggtaat tcaggttgcg
     7381 gtccaggatg gccaagccgc tcgtcaacgc ctgtttccgg gcagcggctg gggtaggcag
     7441 gtccttgcgg cgaagtttgc ggacagcggc ctttttctcg ccctccggca gttgcgcgat
     7501 ttcacgttgc gtttcgaccg gctggtcggc gatcagtgat gcggcactga tggacaggtc
     7561 gcccgagtcc atctgcgcga ctacttcatc gacggccttc tcaacgacct tcttggccaa
     7621 ctcgtaggtg gtgtggcctc cgaatccggc ctttttggca gcgatgtcgc gggtctcgac
     7681 gcctggcaca acttccgccg gatttggcgg aagttccttg tctgtacgct gcccctgccg
     7741 gtctccgatt tccgcttcca gtgccttggc gatagccacg cgctcagacg gcgtgaaatc
     7801 tttgcggatc tcgttctcgg cgtactcgcc ggcgacaatg ctggagactt gcaggatgat
     7861 aacggggatc gtcttccatc gcaggatgtc gcgcatggcc aggaaacgtc gttcgccgca
     7921 gatcaggcga ccgtcttctg taacgaccgg cgggtgcagc agccccacag tggcaatgct
     7981 ggccgccagg gtttccacgt cgcccatgtc cttgcggtga ccgaggcctg tctatcctcc
     8041 cacgtgcaca atgtgcacga ggaggacgat aatgccgcaa cttcggacag gacgacgcca
     8101 acgagaactc ttc
//
LOCUS       DASTMK010000494        11526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_601152, whole genome shotgun sequence.
ACCESSION   DASTMK010000494 DASTMK010000000
VERSION     DASTMK010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601152"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFQ79_20595"
     CDS             complement(<1..263)
                     /locus_tag="VFQ79_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142133.1"
                     /translation="MRKVPLVESLQIVGRGSLNWFRGRPIVVSFEVTDSCNCYCKHCD
                     HGGPRDESRLLRPPDYRRYMEALNPCVVQVSGGEPLLRDDLVE"
     gene            complement(352..3894)
                     /locus_tag="VFQ79_20600"
     CDS             complement(352..3894)
                     /locus_tag="VFQ79_20600"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142134.1"
                     /translation="MGRIAIAFLLLALTPCTVRAQTSAGAVVGVVRDPTGAVIPEARV
                     VVTNTGTNLAFPFVTDATGNYYVPSLIPGSYRVDAEKTGFKRVSVADLTVAVNQTLRV
                     DITMPVGEVAEAVSVTAEAPLIQSDQSTLGQVVNNRAVSELPLNGRDFTALLRLNTGV
                     TEVQGGINAAPTIRRHGLNDAFRNVSVNGTRPASVSYLIDGIATNDGLFQTPAVIPPI
                     DLIQEFKLQNGLYSAEFGMGSAQVNVALKSGVNQLHGSAWEFLRNDALQPSNPKSHTK
                     PSLKQNQFGAAVGGPVWLPGLYNGTDRTFFFGSYQGGRRRTGSIGQVQVPSDRQKQGD
                     FSDWPTQLYDPLSTVANPDGDPPVLRQPFPNNQIPTTRIAPQSQNLLQYWPAPNINCA
                     FPCNNLLRGVTASTRIDGFSARIDHNIGQSDRLFGQFLFQDEHAPQPSVIPLSGNDVK
                     QRGRMIGLTWTHVFSPRTINEARAGFNRLYFIQGYETAFGSINYWKEIGLTNLRDDPA
                     YYALPLISLGTQYAPVGFGGTAPFFNITNTFQWVDSLTMTRGKHSIKAGADIRRNRFF
                     TQSGGQGNGQLTFLGGYTARNPVIPQTAGRPDTGNGFADFLLGYLNNAGSTTAAFRAF
                     DASASRLRNTDYMLYLQDDYRVTPQLTLNIGVRWELHTPFKDITNGGSMFDFAYPGGR
                     VLYRDQNYVEMVNNPILAACCASDTLIKTDWRDIAPRIGLAWRPLRSSNRLVLRAGYG
                     IFYDIQHNYYAAGSLAQNIPFLSPTLINPTAREPVPPLDIRNLFPAPYSIAERTFTLP
                     FCQAPSQSIIDPVTGINTEVRNFCPAGSSQLPDAKIPYNQQWAFNIQVEPVSNLLLEA
                     GYHGSRALRQPIQWIFNQAVLPPEAGNPNNSVSFRSQCPPGTYPDRCSPIQDRVPYTN
                     FASNRFANANMLQSSYHAMTLKAERRFANGFQVLGSFTWSKAVDQFSEIQNVGGAISS
                     IAQYAHRFDLERGLANFDQTRRFVLSWLYELPVGRGKPFLNRGGIVNAFLGGWQTNGI
                     VTFADGTPFTVGCFCGDRSQTGNIFNTHRPDVNGNPLPDGFERTYVKQFDTSVFSTPA
                     LGTLGSSGRNTLRSTGQRAGDISFFKNFQIRERGQIQFRSEFFNLLSSHFYSPRFPSN
                     NLSATNFGSLLPVGGDHGDLFNPRIIQFGLRLLF"
     gene            complement(4019..5365)
                     /locus_tag="VFQ79_20605"
     CDS             complement(4019..5365)
                     /locus_tag="VFQ79_20605"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEU0142135.1"
                     /translation="MNRRSLLRFFAGLASAPALPALGQEDQWKAGVAAVAITPKEPMW
                     MAGYGSRTKPSDGVLHEIFAKALALEDGAGTRSVIVSAEILGFSAAMAETISERVRKK
                     FSMTRERLVLNASHTHSGPVTGDVLLPAYPLDEKEMAIIRRYTARFIDQVVDIVGQSL
                     ANLEPAQLSFEQGFAGFAVNRRRVGARHRPGPVDHDVPVLCVRKAGGAIKAVLFGYAC
                     HNTNLSGYQYSGDYSGVAQAAIEKMYPGASALFVAGCGADANPLPRRTVELATMYGNI
                     LANAVDLVLKSKMRPVAGPLHAAWDYVDLPFQTAPTKEQLQAQVSKGEGMYRRYAEGL
                     LRKIAKEGQLPGKYAYPVQVWQFGRDLTFIPMAGEVVSDYSLRFKNQYGWDDTWVAGY
                     SNDVFAYIPSLRVLKEGGYEGGGAMIPYGQPAPFRAPVEEIIAEKVDELVKRVRTK"
     gene            complement(5370..6974)
                     /locus_tag="VFQ79_20610"
     CDS             complement(5370..6974)
                     /locus_tag="VFQ79_20610"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEU0142136.1"
                     /translation="MMPQLTRRNFLAASATAALAAPSNGLIAHYRLDRDSADFKNHGA
                     VFTPDGARFNGRSAYIEIPDSPALRLGARDFTISAYIHTDSDSGDVAGDIAAKYDPVS
                     RRGFTFSVKTNAVTFSASNERQLQFGIDNGHTGEWQDCGRSGNSIYAMGLIVFDGALY
                     AGTCEPGKNERGHVYRYSGGTGWADCGAPDPSNSISSLAVHRGRLYAGATRYNLGGSA
                     LQPSANDAPGGHVYRYEGGSKWTDCGRPGESISISGMAEYRGRLYVSSLYAPAGTFRY
                     EGGDRWVSCGTPGGRRVEAMAVYNGALYGTGYDAGEVYRFDGKEWVTIGRLPETTQTY
                     GFAIFEGKLYVSTWPTARVFRYDGDDNWVDCGRLGDEKEVMGLAVYNGKMYGGTLPLA
                     EVYRYEAGQTWTRTGQLDKTPDVKYRRAWSMAVYRGKLFCGTLPSGHIYSLEAGKSVT
                     HDRSLAPGWRRVAAVRSGRSLKLYVDGKAVAASTPFEPAGYDISNALPLRIGFGDHDY
                     FNGLIRDVRIYGRALTEAELGELDVH"
     gene            complement(7036..10674)
                     /locus_tag="VFQ79_20615"
     CDS             complement(7036..10674)
                     /locus_tag="VFQ79_20615"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0142137.1"
                     /translation="MRRIAIMLATVYCVSPTLAQETRGAIFGRVLDNQAAVIGRASVR
                     VTNTDTNTTVPLTTNETGYYEASLLLPGNYQVSAEAPGFRTIIRRGIVLTVSSRVEIN
                     LTLEVGAVTDSVTVTAEAPLIDTSSASSGRVVDNRTQAGVPVSSSNITLFARMAPGVQ
                     SNGEVRVIGPGDQASTSDYKVAGNVGSNEWAIDGASNNGSGGRLVGYVVHSDEISEIK
                     IETGGFDSSIGHTSGIVVAMMSKSGTNQFHGGASNIHEQTRWNATPFFTRQLYYRRIA
                     EAEAAGDTALANELRDTPKQLSGKRNHYSGFIGGPVILPKIYDGRDRLFFFFSYVGFR
                     RVIPPNPINFNHTFPTMANREGDFSQLLNADATRYQIYDPLSVRADESRAGHFIRTPF
                     PGNILPRSRIMNPAYDVYKNMLPIPNNDPLSPSAEPLNNYLAVSVPWVSPYWAVTNRI
                     DYKLSDRHRFFGRWSADDYKLLNALDWTYGTPHQGLQASLDRRANVSGVIDWVWTPTA
                     TTYFDVAVTGNQYEGGDFNEVAMNYKPSDVGMPAYMDAKAGDLHTLPHMAFAGYNPVG
                     NYGVTTRTRWRTYALRADGTHIHGRHTLRAGFDVRSHYYAGGAPGLTSGSFSFNNAFT
                     RRNDDTFTPAGDLGHSWAAFMMGLPSGISVASNDNFAATSPYYGWHFQDQWRVNPKLN
                     LNLGLRMEYEGGPVERFNRIIGYFDPSLELPISSAAEAAYARNPLAERPASTFDIRGG
                     TVYPNDNQRAIRDGELMWMPRIAAAYQWNDRTVIRAGFGMFYDTLTAAKETVNQFGFS
                     RNTVSNPTNDFGVNWLVGDPANGISPLLDPFPVRSDGSRFDLPIRDALGSMAYAGRSF
                     TFSPWDTKRARQRRWRIGVQRQLGASMLLEAAYAGAYSDRVYVNTNLSPLPEQYWADG
                     MVRNNDVASRMNENVPNPFQLSNFADLSTSNPVVYQDMSTQGFYTAGTTRRNQLLRQY
                     PHMSGLVARNSPYGEVRTDSMELSFERRFSKGFNFYVAYTRMRGDDRDVYLNEFDALP
                     SWRESNNSRPHRIVLTGIWELPFGKGRPFVRSGPASWIVGGWQLGVAWEAQSGALLDF
                     GNLFYYGDLDDINTGERTFDRWFNTDNFERVSARAPAAFHKRVFPTRIDGLRADPTNC
                     WNGNVMREFKFTERVGFQVRMEALNFTNRTQFAAPDTNPLSSNFGKVTLQSQTNKRYI
                     QLTGRITF"
     gene            10913..>11526
                     /locus_tag="VFQ79_20620"
     CDS             10913..>11526
                     /locus_tag="VFQ79_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142138.1"
                     /translation="MHKSFPITRRAFAGSAALAALPPQAGSARKPAQERYRAVSWWLT
                     ADDVAWPNDELMDRIRRRADRCAASAVNCCLVFGVHFRWDFMPMWGRVHDLLRFIADE
                     LHERDIMLIDHHSSVLTHRARNREEALNISRRNRHHVPFYPTVEAAAEWKFDGRRLND
                     WRMLDVETGKPVYLPAYNAEQFCMNNAEFRQAYGKYVRQLVAETG"
BASE COUNT         2219 a         3187 c         3839 g         2281 t
ORIGIN      
        1 atctcgacca ggtcgtcccg gagaagcggc tcgccgcccg acacctgcac cacgcaaggg
       61 ttcaacgcct ccatatagcg gcggtagtcg ggcggccgca aaagccgcga ttcgtctctc
      121 gggccgccgt ggtcgcaatg cttgcagtag cagttgcagg aatccgttac ttcaaaagaa
      181 acgacgatag gccgtccgcg aaaccagttc agcgaacctc gtccgacgat ttgtagcgac
      241 tcaaccagag gaaccttgcg catataaata gaaacaggtc tctaagacct atattcaagg
      301 tgacacaaaa tcgtagcaac atgtgccacc gggccgcaag atcgctccag gctaaaacag
      361 caaacgcagg ccgaattgaa tgatccgtgg gttgaacagg tccccgtggt cgccgcccac
      421 cggcagcagc gaaccaaagt ttgtcgcgct caggttgttc gacgggaacc ggggcgagta
      481 aaagtggctt gacaacaagt tgaaaaactc ggagcggaac tggatctggc cacgctccct
      541 gatctggaag ttcttgaaga acgatatgtc tcccgcgcgc tggcccgtgg agcgcagagt
      601 gtttcgtcca ctgctaccga gggttcccag tgcgggcgtg gagaaaaccg aggtatcgaa
      661 ctgcttgaca tatgtccgtt cgaatccgtc cggcagcgga ttgccgttca cgtccggacg
      721 gtgcgtgttg aagatgttgc ccgtctggct gcggtcgccg cagaagcagc ccacggtaaa
      781 cggagtgccg tcggcaaagg ttacgatgcc gtttgtctgc catccgccga gaaacgcatt
      841 gacgatgccg ccgcggttga gaaacggttt tcccctgcct accggcagct cgtacagcca
      901 gctcagcacg aaacgcctgg tctgatcgaa attcgcgagc ccgcgttcca ggtcgaagcg
      961 gtgtgcatat tgggcgatgg aggagatggc cccgccgacg ttctgaattt cggaaaactg
     1021 gtcgacagcc ttgctccatg tgaaggagcc aagcacctgg aagccgttcg caaaccgccg
     1081 ctcggctttg agcgtcatgg cgtgatagga cgactgcaac atgttcgcgt tggcaaagcg
     1141 attggaggcg aagttggtat agggaaccct gtcctgaatg ggtgaacacc ggtcggggta
     1201 agtgcccgga ggacattgtg agcggaacga cacgctgttg ttcgggtttc ccgcttccgg
     1261 aggcagaacc gcctggttga aaatccattg aatcggctgc ctcagtgcgc gagagccgtg
     1321 gtagcctgcc tccaatagca ggttcgaaac cggctcaacc tgaatattga atgcccactg
     1381 ctggttatag ggaattttgg catcgggcaa ctgcgaggaa ccggccggac agaagttccg
     1441 gacttcggta ttgatgcccg tcaccggatc gatgatggat tgcgacggcg cctggcagaa
     1501 cggcaaagtg aacgttcgct ccgcaatcga ataaggagct ggaaacaggt tccggatgtc
     1561 caggggtgga accggctccc tcgccgtcgg attgatcagc gtcggtgaaa gaaacgggat
     1621 gttctgtgcg agcgaaccgg ccgcgtagta gttgtgctgg atgtcgtaaa aaatcccgta
     1681 gcccgcacgc aacaccagcc ggttgctcga acggagaggt ctccaggcga gtccgatccg
     1741 cggagcgatg tcgcgccaat cggttttgat cagagtgtcc gaagcgcaac aggccgcgag
     1801 gatcggattg ttcaccatct cgacatagtt ttgatcgcgg tacagcaccc gtccgccggg
     1861 gtaggcgaaa tcgaacatgg agccgccgtt ggtgatgtcc ttgaacggcg tatgcagttc
     1921 ccagcgcacg ccgatattca gcgtcaattg gggcgtgaca cgatagtcgt cctggagata
     1981 cagcatgtaa tccgtgttgc gcagccgcga tgccgacgcg tcgaacgccc ggaaggcggc
     2041 ggtagtcgat cccgcgttgt tcagatatcc caaaagaaaa tcggcgaagc cgttgccggt
     2101 gtcgggtctt ccggctgtct gtggaatgac gggattcctc gccgtgtatc cgcccaggaa
     2161 ggtcaactgg ccgttaccct ggcctccgct ctgggtgaaa aagcggttac gccggatgtc
     2221 tgcgccggct ttgatactat gcttaccgcg cgtcatcgtg aggctgtcca cccactggaa
     2281 tgtgttcgtg atgttaaaaa acggagcggt accgccgaag ccgacggggg cgtactgggt
     2341 tcctagcgag atcagcggaa gcgcataata ggccggatcg tcccgcaggt tggtgagccc
     2401 gatttccttc cagtaattga tggaaccgaa ggccgtctca tagccctgga tgaagtaaag
     2461 acggttgaat ccggcgcgcg cttcgttgat cgtgcgcggg ctgaagacgt gagtccaggt
     2521 caggccgatc atgcggcccc gctgtttcac gtcattgccg gagagcggga tgacggatgg
     2581 ctgcggagca tgttcgtcct gaaacaggaa ttgtccgaac agccggtcgg actggccgat
     2641 gttgtgatcg attcgcgcgc tgaatccgtc gatccgcgtg gaggcggtga ctccacgaag
     2701 cagattgttg cacgggaaag cgcagttgat gttcggtgcg ggccaatact gcaagagatt
     2761 ctggctttgc ggagcgattc gtgtagtggg gatctgattg ttcggaaaag gctggcggag
     2821 gacgggtggg tcgccatccg ggttggcgac cgtgcttaaa ggatcgtaaa gctgcgtagg
     2881 ccagtcggag aagtcgccct gcttctggcg atcggacggt acttgtacct gcccgatgga
     2941 acccgtacgc cgccgcccgc cctggtagct tccgaaaaag aacgtgcgat ccgttccatt
     3001 gtagagaccc ggcagccaga ccgggccgcc cactgccgcg ccgaactggt tctgcttcag
     3061 cgaaggcttg gtgtggctct tcgggttcga tggctggagc gcgtcgttgc gtagaaattc
     3121 ccatgcggag ccgtgaagct ggttgacgcc cgacttcagc gcaacattca cctgcgccga
     3181 gcccatcccg aactccgccg aatacaaccc gttctgcagc ttgaattcct gaatcaggtc
     3241 gatgggagga ataacggcgg gtgtctggaa cagaccgtcg ttcgtagcga tgccatcaat
     3301 gaggtaactt acggatgccg gacgagtgcc attcacgctg acattgcgga aagcgtcgtt
     3361 caagccgtga cgccggatgg taggcgcggc gttgatgccg ccctgcactt ccgtcacgcc
     3421 ggtattcagg cgcagaaggg cggtgaagtc gcgtccgttc agcggaagct ccgagacggc
     3481 gcgattattc actacctgcc caagtgtcga ctgatcggat tgtatgagcg gcgcttccgc
     3541 tgtcacactg accgcctcgg cgacttcacc gacaggcatc gtgatatcca cgcggagcgt
     3601 ctggtttacg gcaaccgtga ggtccgccac gctgacccgt ttgaatcccg tcttctctgc
     3661 gtctacccgg tagctgccgg gaatcagcga ggggacgtag tagttgcccg tggcgtccgt
     3721 cacgaatggg aatgctaaat tcgtgccagt gttggtaacc actactctgg cttcggggat
     3781 gacggcgcca gtcggatccc gtactactcc aacaacggcc ccggcagaag tctgtgcgcg
     3841 cactgtacac ggtgtgagcg ccagcaacag gaaagcgatg gcaatccggc ccataggccc
     3901 tcccagaact acttaaggga agcctactcc cgtgcaaggg ccacgtcaac gatgattcga
     3961 tacttgtaac aagctcgttc gcggatgtta caagaccacc gccgcacact gccggagctt
     4021 atttggtccg gacgcgcttc accaactcgt ctaccttttc agcgattatc tcttcgaccg
     4081 gggcgcggaa gggcgcgggt tggccgtagg ggatcatcgc tccgccacct tcgtagccgc
     4141 cttccttcag gacgcgcagc gaggggatgt aggcgaagac gtcgttcgag tagcccgcga
     4201 cccatgtatc gtcccacccg tattggtttt tgaagcgcag ggagtagtcg gacacgacct
     4261 cgcctgccat gggaatgaaa gtaaggtccc tgccgaactg ccagacttgc acggggtagg
     4321 catacttccc gggcagttgg ccttctttcg cgatctttcg gagaaggccc tcggcgtagc
     4381 ggcggtacat gccctcgcct ttgctcacct gcgcctgcag ttgttctttc gtcggggctg
     4441 tctggaaagg caggtccacg tagtcccaag cggcatggag gggacccgcg accggtctca
     4501 tcttgctttt tagaacgagg tccaccgcgt tggcgaggat gttgccgtac atggtggcga
     4561 gttccacggt gcggcgcggc agggggtttg cgtcagcgcc gcacccggcg acgaaaagcg
     4621 cactcgcacc cgggtacatt ttctcgatcg cggcctgtgc gactccggag tagtcgccgc
     4681 tgtattgata tccggacaga ttggtgttgt ggcaggcgta gccgaacagc acggctttga
     4741 tggcgccgcc tgctttgcga acacaaagaa ccggcacgtc gtgatcgacg ggaccgggac
     4801 ggtgccgcgc gccgactctc ctgcggttga cggcaaaccc ggcaaagccc tgttcgaagg
     4861 agagttgagc gggttcgagg tttgcgagcg actgccccac gatatccacg acctgatcga
     4921 tgaagcgggc ggtgtagcgc ctgataatgg ccatctcctt ttcatcgagc gggtaggcgg
     4981 gcaacagcac atccccggtg acagggccgc tatgcgtatg gctggcattc agaaccagac
     5041 gttcgcgcgt catactgaac ttcttccgaa ctcgttcgct gatcgtttcg gccatagcgg
     5101 ccgaaaagcc gaggatttcg gcggacacga tgaccgaacg ggttccggct ccatcttcga
     5161 gcgcgagcgc cttggcaaag atctcgtgga gaactccatc cgaaggcttg gtgcgggagc
     5221 cgtagcccgc catccacatg ggctctttcg gcgtgattgc cacggctgca acgcccgcct
     5281 tccactggtc ctcctgccca agcgcgggca gcgctggagc ggatgcaagc ccggcgaaga
     5341 agcgcagcag ggaacggcga ttcattccgt tagtgaacat ccagctctcc taactcagcc
     5401 tccgtcaaag cacggccgta gatccgcacg tcgcggatca aaccgttgaa atagtcgtga
     5461 tcgccgaatc cgatccggag cggcagagcg ttcgagatgt cgtaaccggc gggttcgaac
     5521 ggcgtcgagg cagccacagc tttgccatcg acgtacaatt tcaggctccg gccgctgcgg
     5581 accgcggcca cgcgtctcca accgggtgcg agggaacggt cgtgtgtgac gctctttcca
     5641 gcctccagtg agtagatgtg gccggacggc agcgtaccgc agaagagctt tccccgatag
     5701 acagccatgg accaggcgcg acgatacttc acgtccgggg tcttgtccaa ctggccggtg
     5761 cgcgtccagg tctgccccgc ttcatagcgg tagacctcgg cgagcgggag ggtgccgccg
     5821 tacattttgc cgttatacac cgcgagtccc attacttctt tctcgtcgcc gaggcggccg
     5881 caatcgaccc agttatcgtc gccgtcgtag cggaagacgc gcgcggtggg ccaggtggag
     5941 acgtacagtt tcccttcgaa gatggcgaag ccgtaggtct gcgtggtttc aggcagacgg
     6001 ccgatggtaa cccactcttt accatcgaag cggtagactt cgccggcgtc gtagccggtc
     6061 ccgtagagcg cgccgttgta gacggccatg gcttcgacac gccgtccgcc gggcgtgccg
     6121 caggagaccc acctgtctcc gccttcatag cgaaacgttc cggcgggcgc gtagagggaa
     6181 cttacataga ggcgtccccg gtactcggcc attccggaga ttgagatgga ttcgccgggg
     6241 cgtccgcagt ccgtccactt gctgccgcct tcgtagcggt aaacatggcc gcctggggcg
     6301 tcgttggcgg aaggctgcaa agcggagccg ccgaggttat agcgcgtggc gccggcatag
     6361 agcctgcccc gatgtaccgc gagcgacgag atggaattgg acgggtccgg tgcgccgcag
     6421 tcggcccagc cggtgccgcc cgagtaacgg taaacgtggc cccgttcatt cttgccgggc
     6481 tcgcaggttc cggcgtagag cgcaccgtcg aagacgatga gacccatggc gtagatcgag
     6541 ttgccggagc gtccgcaatc ctgccactcg ccggtatggc cgttgtcgat gccgaactgc
     6601 agttggcgtt cgttggaggc gctgaaggtg acggcgttgg ttttgacaga gaaagtgaag
     6661 ccgcggcggg agacggggtc gtacttggcg gcgatatcgc cggctacgtc gccggaatcg
     6721 gaatccgtgt ggatgtaagc ggagatggtg aagtcgcgcg cgccgaggcg cagggcgggg
     6781 gagtccggga tttcgatata ggcggagcgc ccgttgaagc gggctccgtc cggggtgaaa
     6841 acggccccgt ggtttttgaa atcggcggag tcgcggtcaa gccgatagtg ggcgatgagg
     6901 ccgttcgaag gagcagccag ggcggcagtg gcgctggccg cgagaaagtt gcgccgggta
     6961 agttggggca tcattgtgtg tgtgaggcgg ggagggccga agccctcccc caagctgaag
     7021 tccgccttat ggttttcaga atgttatgcg ccccgtgagt tggatgtagc gcttgttggt
     7081 ttgcgattgc agcgtgacct tgccgaaatt tgaagacaac gggttcgtgt cgggagcggc
     7141 gaactgggtg cggttagtga agttgagggc ttccatgcgg acctggaagc cgacgcgctc
     7201 ggtgaacttg aattcgcgca tgacgttgcc gttccagcag ttggtggggt cggcacgcag
     7261 accgtcgatg cgggtcggga agacgcgctt gtggaaggcg gcgggagcgc gagcggaaac
     7321 gcgctcgaaa ttgtccgtgt tgaaccagcg atcgaaggtg cgctcgcccg tgttgatgtc
     7381 gtcgaggtcg ccgtagtaga agagattgcc gaagtcgagg agcgcgccgg attgcgcctc
     7441 ccaggctacg ccgagctgcc agcctccaac gatccaactg gcgggaccgc tgcggacgaa
     7501 aggccgtccc ttaccgaacg gcagttccca aatgccggtg aggacgatgc gatgcggacg
     7561 gctgttgttg ctttcgcgcc aggagggcag ggcatcaaac tcgttcaaat aaacgtcgcg
     7621 gtcgtcgccg cgcatgcgag tgtaggcgac gtagaagttg aagcctttcg agaagcgccg
     7681 ttcgaagctg agttccatgc tgtcggtgcg tacctccccg taaggactgt tgcgggccac
     7741 caatccgctc atatgcggat attggcggag caactgattg cgccgggtgg tacctgcggt
     7801 gtagaagccc tgcgtgctca tgtcctgata cacgacgggg ttcgacgtgc tcaggtcggc
     7861 gaagttggag agttggaacg gattgggcac gttctcgttc atccggctgg ccacgtcgtt
     7921 gttacggacc attccgtcgg cccagtactg ttcgggcagg ggcgagaggt tggtgttgac
     7981 gtacacgcga tccgagtaag cgccggcata ggcggcttcg aggagcatgc tggcgccgag
     8041 ctggcgctgc acgccgatac gccaccggcg ctggcgggcg cgcttggtat cccacgggct
     8101 gaaagtgaaa ctgcgtccgg catatgccat gctgcctagg gcgtcgcgga tagggagatc
     8161 gaaacgggat ccgtcggagc gtacagggaa cgggtcgagc agcggtgaga tgccgttagc
     8221 gggatcgccc acgagccagt tcacgccgaa atcgttcgta ggattgctaa cggtattccg
     8281 gctgaacccg aactggttga cggtttcctt ggcggcggtg agcgtgtcgt agaacatgcc
     8341 gaagccggcg cggatcacgg tccggtcgtt ccactggtag gcggcggcga tgcgcggcat
     8401 ccacatcaat tccccgtcgc ggatggcgcg ttgattgtcg ttcggataaa ccgttccacc
     8461 gcgaatgtcg aaggtagaag cgggcctctc ggccagtgga ttccgcgcgt aggccgcctc
     8521 ggcggcgctg gatatcggca gttccagcga gggatcgaag tagccgatga tgcgattgaa
     8581 gcgctccacc ggtccgcctt cgtactccat gcgaagcccg aggttcagat tcagtttggg
     8641 attgacgcgc cactggtcct ggaagtgcca gccgtagtac ggggaggtcg cggcgaagtt
     8701 gtcgttggag gcgacggaga taccgctcgg gagccccatc atgaaagcgg cccaggagtg
     8761 tccgagatcg ccggcgggag tgaaagtgtc gtcgttgcgg cgcgtgaatg cgttgttgaa
     8821 agaaaagctg ccggacgtca atccgggtgc gccgccggcg tagtagtgcg agcgaacgtc
     8881 gaatccggcg cgcagcgtgt ggcggccatg aatgtgcgtg ccatccgccc tcaaggcgta
     8941 cgtgcgccag cgagttctgg tggtgacacc gtagttgccg acagggttgt agccggcgaa
     9001 ggccatatgc ggaagcgtgt ggaggtcgcc ggctttggcg tccatgtagg caggcatgcc
     9061 cacgtcgctc ggtttgtagt tcatggccac ctcgttgaaa tcgccccctt cgtactgatt
     9121 gccggtaacc gcgacgtcga aatacgtggt cgcggtggga gtccacaccc aatcgattac
     9181 gccggatacg ttggcgcgcc ggtcgaggct ggcttgcagg ccctggtggg gcgttccgta
     9241 ggtccagtcg agtgcgttca gcagtttgta gtcgtccgcc gaccagcgtc caaagaaacg
     9301 gtggcggtcg gaaagcttgt aatcgatgcg gttggtgacg gcccaataag gcgagaccca
     9361 gggaacgctg acagcgagat agttgttgag cggttcagcg cttgggctca gaggatcgtt
     9421 gttcgggatg ggcagcatgt ttttgtagac atcgtaggcc gggttcatga tgcgcgagcg
     9481 cggcaggatg ttgccgggga agggcgtgcg gatgaaatgt ccggcgcggc tttcgtcggc
     9541 gcgcacgctc agcgggtcgt agatctggta gcgggtggcg tcggcattca ggagttgcga
     9601 gaagtcccct tcacggtttg ccatggtggg gaacgtgtgg ttgaagttga tgggattcgg
     9661 aggaatgacc cggcggaagc cgacatagct gaagaagaag aacagcctgt ctctgccgtc
     9721 gtagatcttt gggagaatca ctgggccgcc gatgaagccg ctgtagtgat tcctcttgcc
     9781 tgaaagctgt ttgggcgtgt cgcgaagttc attggccagg gccgtgtcgc cggcggcttc
     9841 cgcttcggcg atgcggcggt agtagagttg gcgcgtgaag aagggcgtgg cgttccagcg
     9901 cgtctgttcg tggatgttgc tggcgccgcc gtggaattga ttggttccgg atttcgacat
     9961 catggcgacg acgatgccgg aagtatggcc gatggaggag tcgaagccgc cggtctcgat
    10021 cttgatttcc gagatctcgt cggagtggac gacgtagccg acgagacggc ctccgctgcc
    10081 gttgttcgat gcgccgtcga tggcccattc gttgctgccg acgttgccgg ctaccttgta
    10141 gtccgaagtc gaagcctggt cgcccgggcc gatgacgcgc acttcgccgt tgctctgaac
    10201 gccgggcgcc atgcgcgcga agagggtgat gttcgacgaa gagacgggaa ctcccgcctg
    10261 ggtgcggtta tcgacaacgc ggccggacga agcggacgaa gtgtcgatca ggggcgcttc
    10321 ggcggtgacg gtgacgctgt cggtaaccgc gccgacctcg agcgtgagat tgatctcgac
    10381 gcggctggaa acggtcaata cgatgccgcg ccggatgatg gtgcggaacc cgggcgcctc
    10441 cgcgctgacc tggtagttgc cgggaagcag cagactcgct tcgtagtagc cggtctcgtt
    10501 ggttgtcaat ggaacggtcg tgttggtgtc ggtattggtg acgcggacgc tggctctgcc
    10561 gatgacagcc gcctggttat ccaggacgcg cccgaagatc gcgccgcgtg tctcctgcgc
    10621 gagcgtggga gacacgcagt atacagtagc aagcatgatg gcaattcgcc tcatggttcc
    10681 ctcgattcac ttgcagcaaa gttgacagca gcctctgagg gcacagtata tagcagcaga
    10741 acccgaaccg gagatttgat tggccacgga tggacacaga tgaacacaga taaagaaaaa
    10801 cgtaaggcat gatccgtgtg aatccgtgtt catccgtggc cgaatttgct tttctagatt
    10861 gctgggatac gccaatcatt tctgacgtca ggtttagaat tgggacaagc gtatgcataa
    10921 atcttttccc atcacgcgcc gggcgtttgc cgggtcggcc gcactcgccg cgctgccgcc
    10981 gcaggcggga agcgccagga agccggcaca ggaacggtat cgcgccgtca gttggtggct
    11041 gacggcggac gacgtagcgt ggccgaacga cgaactgatg gacaggatcc gccggcgggc
    11101 ggaccgttgc gcggcgagcg cagtcaactg ctgcctggtt ttcggcgtgc atttccgctg
    11161 ggacttcatg ccaatgtggg ggcgggtgca tgacctgctg cggttcatcg cagacgaact
    11221 tcacgagcgg gacatcatgc tgattgacca tcactcgtcg gtgctgacgc accgggcgcg
    11281 caaccgggag gaggcgctga acatatcccg ccgcaaccgg caccacgtgc cgttctatcc
    11341 gacggtggag gcggcggcgg aatggaagtt tgatgggcgt cgcctgaacg actggcggat
    11401 gctggatgtg gaaaccggaa aacccgtgta tctgccggct tacaacgcgg agcagttctg
    11461 tatgaacaac gcggaattcc ggcaggcgta cgggaagtat gtccggcaat tggtcgccga
    11521 gacggg
//
LOCUS       DASTMK010000495         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_184956, whole genome shotgun sequence.
ACCESSION   DASTMK010000495 DASTMK010000000
VERSION     DASTMK010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184956"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFQ79_20625"
     CDS             complement(<1..489)
                     /locus_tag="VFQ79_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142139.1"
                     /translation="MTRRNLIASSALAASFAPSGAAAKAARSIIEMRVAKLRNGSDNQ
                     MQRLGEFIGKAAVPALKRAGAGPVGAFASLIAEDSPFLLVVTSYPTLSAMESVAQKMA
                     ADKEYIEAARKFYSAPGLPFQRMECSLLRGFDSMPNIEVPPTEGRKTPRVFEIRTYES
                     NTS"
     gene            559..2064
                     /locus_tag="VFQ79_20630"
     CDS             559..2064
                     /locus_tag="VFQ79_20630"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEU0142140.1"
                     /translation="MPRRATLADVEIDVETGPKPVALETDPGDPFRILLLGNFSGRRN
                     RGPSERVSTERRPVLVDRDNFEAVMKKMAPRVTLAPETVDAPELDLQFTDLDHFHPDH
                     LYDSLPVFAALRELRKKADDPAAFRTLAAEFAPSSPSQPAPAVPEPEAFAAGSLLDQI
                     LENEPRSSGAAASRRGDPMASFLRSIVAPYLVPGRDPKQDDLVAHIDNTTSARMRAIL
                     HAADFQALESLWRVVFFLTRRLETGTDLKLYLLDLTREEFAETLAGDDLRRSELYRLL
                     VDEVSVSGTPPWAVIATDYTFDNSIEDVELLGRIALIAERARAPFLGRAHERVLGISS
                     LASSPDPHSWSTGNDPAWSILRELPESRWLALALPRFLLRLPYGKETSETEQFAFEEM
                     EAAVRHESYLWGNPGFACAMALGQAFSEYGWGMEPGAVLEVGGLPVHTFLEDGTPAMK
                     PCAEVWLTDKAIEVITAKGLIPLISKKGSDSVRLGLFQSVCGTKLGGRWAS"
     gene            complement(2057..>2978)
                     /locus_tag="VFQ79_20635"
     CDS             complement(2057..>2978)
                     /locus_tag="VFQ79_20635"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0142141.1"
                     /translation="TAQDHIDDVTDMVRQAHNDIEKRRAEQAIEWDQLQQSLRVAKAD
                     LDGWTLEAGAIELRTPIDKELIELQVEESAARYKQLQEDLASKKIAHAAELRILEITS
                     ERQERHLDRHVTDIKRLTIHAPMDGLAVMESIWRGQEMAQVQEGDLVTPGQRIMKIVD
                     PTNMQIEGTVNQAEIGDLRVGALAHIGVDAFPGAKFKGKVYSIGALATGNRRAGDYVR
                     NVPVKVVIDGSDPRVIPDLSAWADVIVEQKDDATIIPLSAIQNEGGQSFVRVKSGIGF
                     AKRPVKVGLTNTTHAAITEGVNPGDEVRIN"
BASE COUNT          563 a          895 c          872 g          648 t
ORIGIN      
        1 gctggtattc gattcgtaag tgcgaatctc gaatacccgg ggggtcttgc ggccctccgt
       61 tggcggcact tcgatgttgg gcatggaatc gaagccgcgc agcagggaac actccatacg
      121 ctggaacggc aagccaggcg cgctataaaa cttccgggcg gcctcgatat attccttgtc
      181 ggcggccatc ttctgggcta ccgactccat cgcagaaagc gtgggataac tggtgacgac
      241 cagcagaaaa gggctgtctt cggcaatgag gctggcgaac gcgcccaccg gtccggctcc
      301 ggcgcgtttg agcgcgggaa ccgctgcctt tccgatgaat tccccgaggc gttgcatctg
      361 attatcacta ccgttccgca gtttggcgac gcgcatctca ataatggagc gggccgcctt
      421 agccgccgca ccggacgggg caaacgaggc ggcaagcgcg gaagacgcga tgaggtttcg
      481 gcgagtcata taaatcgact gtacaacagc gcgttcctcc gtgacttgca cggggttcca
      541 ggcgcgtata atactcccat gccgagacga gccacgctcg ctgacgtgga gatcgacgtt
      601 gaaaccgggc ccaagcccgt ggcgcttgaa acggacccgg gcgatccttt tcgcattctc
      661 ctgttaggaa acttcagcgg ccgccggaac cgcggccctt ctgaacgcgt gtccacggag
      721 cgccgtcccg tattggtgga ccgcgacaat tttgaagccg tcatgaaaaa aatggcgccg
      781 cgcgtaacgc tggcgccgga gacggtggat gctccggaac tggaccttca gttcacagat
      841 ctggaccatt tccatcccga tcatctctat gattcgctgc cggtcttcgc cgcgcttcgc
      901 gagttgcgga agaaagcgga cgatccggcg gcattccgga cgctggcggc agagtttgcg
      961 ccatcctctc cctcccaacc cgcacccgcc gtccccgaac ccgaagcttt cgccgcgggc
     1021 agcctgctgg atcagatcct cgagaacgaa cctcgaagca gcggagcggc tgcctcccgc
     1081 cgcggcgatc ccatggcatc gtttctccgg agcattgtgg ctccttacct ggtaccgggc
     1141 cgcgatccga aacaggatga tctcgtcgca cacatcgaca acacgactag tgcgcggatg
     1201 cgggctattc tgcatgccgc cgattttcaa gcgttggagt ccctctggag agtggtgttt
     1261 tttctcaccc gccgcctgga gaccggaacc gatctcaaac tctaccttct ggatctgacc
     1321 cgggaagagt ttgcggagac gctggcggga gacgatttga gaagatcgga actctaccgc
     1381 ctgttggtcg atgaggtttc cgttagcggt actccgccgt gggcggtcat cgcaacggat
     1441 tacacgttcg acaattcgat cgaagacgtg gaactactcg gccggatcgc actcattgcg
     1501 gaacgcgcac gcgctccttt tctcggacgg gcgcatgagc gggtgctcgg catttcttcc
     1561 ctggccagtt cgcccgatcc gcactcctgg tccaccggaa acgatccggc gtggagcatc
     1621 cttcgcgaac ttccggaatc acgctggctc gccctggcac tgccacgttt tttgttgcgt
     1681 ctgccttacg gcaaagagac gtcggaaacg gaacagttcg catttgaaga gatggaggcc
     1741 gctgtccgcc acgaatcgta cctctgggga aaccccggct ttgcctgtgc gatggcgctg
     1801 ggacaggcgt tctcggaata cggttggggc atggaaccgg gcgctgtgct ggaggtaggt
     1861 gggctgccgg ttcacacgtt cctggaggat ggaacaccgg caatgaagcc ttgcgcggag
     1921 gtgtggctta ccgataaagc gatcgaggtg attacggcca aaggtctaat tccgctgatc
     1981 tccaaaaagg gtagcgattc ggtgcgcctc ggcctttttc agtcggtgtg tggaacgaaa
     2041 ctcggcgggc gttgggctag ttgatccgta cttcgtcgcc gggatttaca ccttccgtaa
     2101 tggctgcgtg ggtcgtgttt gtcaatccta ccttcaccgg acgcttggcg aatccgatgc
     2161 cggactttac acgaacgaac gactgcccgc cttcgttttg gatggccgaa agcggaatga
     2221 tcgtggcatc gtccttttgc tcgacgataa cgtccgccca ggcggagaga tctggaatca
     2281 cgcggggatc ggagccatcg atgaccacct tgacaggcac gtttcgcacg taatcgccgg
     2341 cacgccggtt tcccgttgcc agcgctccga tactatatac tttgcctttg aatttcgctc
     2401 ccggaaacgc atccacgccg atgtgagcca gtgcaccaac gcgcagatcg ccgatttcag
     2461 cctggttcac agtgccttcg atctgcatat tggtgggatc cacgatcttc atgatccgct
     2521 gtcccggagt aacgagatca ccttcctgaa cctgggccat ttcctgaccg cgccagatgg
     2581 actccatcac cgccaggcca tccatcggcg catgaattgt cagtcttttg atatccgtga
     2641 cgtgccggtc gagatgccgt tcctgccgct cggaagtaat ctccaaaata cgtaattcgg
     2701 cggcatgggc gatctttttc gaagcgagat cttcctgcaa ctgcttgtag cgcgcggcgg
     2761 attcctccac ctgcaattca atcagttcct tgtcgatcgg cgttcgcagc tcgatagcgc
     2821 cggcttcgag ggtccacccg tcgagatccg ctttggcaac acgcaacgac tgctggagtt
     2881 gatcccattc aatcgcctgc tccgctctgc gcttttcaat gtcgttgtgg gcctgccgca
     2941 ccatgtcggt cacatcgtcg atgtgatcct gtgcggtc
//
LOCUS       DASTMK010000496         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_441180, whole genome shotgun sequence.
ACCESSION   DASTMK010000496 DASTMK010000000
VERSION     DASTMK010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441180"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="VFQ79_20640"
     CDS             complement(<1..1952)
                     /locus_tag="VFQ79_20640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEU0142142.1"
                     /translation="MIDKILAWSIHYRYLVVSLTALLAAAGIFSALDMPIDAVPDVTT
                     NQVQINTKAPSFTPLEMEQYVTFPIEVAMSNLPQKESVRSISQFGLSQVTVVFEDGMD
                     IYRARQLVLERLLEAQQQLPEGIIPELAPVSTGLGEIVQFTLKVAPGSARTYGLKELR
                     TLLDWFIKPQLRMVPGVVEVNSFGGEEQQYEVLVNPAKLVSHNIPLQQVSEALRRNNL
                     NVGGAYLERGGEQQLIRGVGLIEDVRDIENIVVGAANGTPVYIHSVADVRSGAQIRQG
                     AATRDGKGETVMGVAMMLKGENSRQVSGRVVQRLNELNKAMPPGVSIDIYYDRRELVD
                     RTVKTAFNNLAEGGLIVIAVLFLFLLQLRAGLIVSSAIPLSMLAAIAGMRYFNISANL
                     MSLGAIDFGLIVDAAVIIVENCVRRLTDERVKLGRSLTPEERRAAILAASVEVRKASQ
                     FGEILIIAAYLPIVSLVGMEGKMFRPMGLTVVLALSAALVLSLTLIPALCATFLRESK
                     RPVRPASEAEDHRHAEENPIVRRLRLWYAPILDLTMRRRALTIAAALAVVAGCVALVP
                     FLGSEFLPKLEEGALAIHAVRLPSASLSESVRMVSSLEQALAEFPEVITTVSRIGRPE
                     IATDPMGVNLSDTYVLLRPKKEWRSAT"
     gene            complement(1949..3157)
                     /locus_tag="VFQ79_20645"
     CDS             complement(1949..3157)
                     /locus_tag="VFQ79_20645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0142143.1"
                     /translation="MKWLVLVVALSLACCAGKEDAAREEPAEGQRTNDEAHPEKYLEE
                     ANVVELSLDAQKRAGIEVAPVRMRTVQDEVRVTGTVQPIESRIVHLRPLARGRVEQVV
                     AKVGDRVRKDQVLAHLDNIEAGELSSQIATAQAELQRLKIQHANARRQAGRSRSLVAI
                     GAVPAKEAEAAETEARALEEAIRAQESAIAGIEMRLKRFGVPAGADPSSTTIRTPFAG
                     VVINAEAAPGDVADTGSVLFAVADLSRVYVEAQVYEKDLGKIQIGQSASITVDAYPGQ
                     TIQGCIATIRDILNPQTRTAAVRFEVPNRDGRLRLEMFTNLAIPTTDTHTALALPVDA
                     IQTINRRQIVFVRKAELHFEAREVQILGDGALTEITAGLKEGEPVVVKGAFQLKSAFL
                     AKQLESEHGH"
     gene            complement(3154..>3888)
                     /locus_tag="VFQ79_20650"
     CDS             complement(3154..>3888)
                     /locus_tag="VFQ79_20650"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0142144.1"
                     /translation="VEAQRSVASGRMEAARSDISRLIGVASGETWTAAAGAVPSTIPL
                     EGLASKAAGQRPDLRLLRFARQFAEAEISLAEAEARPNVMASVSYSRVTSRFDDQLGL
                     NAANQLTPLRDRDDLLQVGISIPLFSRNRNLGNIEAAAARTRAAQHRLEYLERAIPLE
                     VEAAWRQFESSRTALASLDGQAISLSESNLDVIRQAYQLGQLRLLDVLAEQRRALDLQ
                     LTSIDLQAELRRSLAELEKAVGGPLQ"
BASE COUNT          783 a         1205 c         1087 g          813 t
ORIGIN      
        1 gtggcagatc gccactcctt tttcggcctg agcaggacat aggtgtcgct caaattgacg
       61 cccatgggat ctgtggcgat ctcgggccgg ccgatccggc tcacggttgt gatcacttcg
      121 ggaaactcgg ccaaagcctg ctcgagtgaa ctgaccatcc ggaccgactc cgaaagggag
      181 gcactgggca agcggacggc gtgtatcgcc aatgcgcctt cctccagctt gggcagaaac
      241 tccgaaccga gaaatggaac caatgcgacg catcctgcga cgactgcgag ggcggcagca
      301 attgtcaggg cgcgccggcg catggtcaga tcgaggattg gcgcgtacca aagccgaagc
      361 cgccgaacga tcgggttctc ttccgcatga cgatggtctt ccgcttccga tgccggccgg
      421 acaggccgtt tgctttcgcg caaaaaggtc gcacaaagcg ccggtattag cgtaaggctc
      481 agaaccaggg ccgctgacag ggcgagcacg accgtaagac ccatgggacg aaacatcttg
      541 ccctccatgc ccactagcga aacaattgga aggtatgcgg cgatgatcag gatctctccg
      601 aactggctcg cctttcgcac ctccacgctg gctgcaagga tcgccgcccg ccgctcctcc
      661 ggcgtgaggg atcgcccaag cttcacacgc tcatccgtca ggcggcggac acaattttcc
      721 acgataatca cggccgcatc gacgatcagg ccgaagtcga ttgcgccaag gctcatcaaa
      781 ttcgcagaga tgttgaagta gcgcatcccg gcaatggccg ccagcatcga caacgggatg
      841 gcggaggaaa caatcaaacc cgcgcgaagc tggaggagga acaggaacag aacggcgata
      901 acgatcagcc cgccctcggc gaggttattg aatgctgttt tgacggtgcg gtcgacaagc
      961 tcgcgccggt cgtagtaaat gtcgatactg actcccggcg gcatagcctt attgagctca
     1021 ttgagacgct ggacgacgcg gccggacacc tgtcggctgt tctcgccttt gagcatcatc
     1081 gcgacgccca ttacggtttc gcctttaccg tccctcgtcg cggcgccttg ccggatctgg
     1141 gcgccgctgc ggacatccgc cacggaatgg atgtataccg gagttccgtt ggccgcgccc
     1201 actacgatat tttcaatgtc ccgaacgtct tcaatcagcc ctactccgcg aatcagttgc
     1261 tgttcaccac cgcgttcgag gtaggcgcct ccaacattca aattgttccg ccgtaaagct
     1321 tccgatacct gttggagtgg gatattatgg ctgaccagct tcgccggatt caccaggact
     1381 tcgtattgct gttcctctcc tccaaagctg ttcacttcca caacgccagg caccattcga
     1441 agttgaggtt tgatgaacca gtcgagaagc gtacgcagtt ctttcagacc gtacgtccgg
     1501 gcggagcctg gagccacctt cagggtgaac tgcacaatct cgcccaaccc tgtgctcacc
     1561 ggagccagtt cgggaatgat tccttcagga agttgctgtt gtgcctccag caaacgttcc
     1621 agaaccaact gccgggcccg atagatgtcc atgccatctt cgaacacgac ggttacctgc
     1681 gacagcccga actgtgaaat ggatcgaacg ctttccttct gggggagatt gctcattgcc
     1741 acttcgatcg ggaacgtaac gtattgctcc atttcgagcg gtgtaaacga gggtgccttg
     1801 gtgttgattt ggacctggtt ggtggtgacg tccggaacgg cgtctattgg catgtcgagc
     1861 gccgagaaga tgccggccgc tgccagcaac gccgtcaacg acacgaccag atagcggtaa
     1921 tgaatgctcc aagcgagaat tttgtcgatc aatgcccgtg ctccgattca agctgctttg
     1981 caagaaacgc agacttcagt tgaaacgctc ccttaaccac aaccggttct ccctccttga
     2041 ggcccgccgt aatctcggtc aaagccccgt cgccgagtat ttgcacctca cgagcctcga
     2101 aatgaagctc cgccttgcgg acaaacacga tctgtcttcg attgatcgtc tggatagcat
     2161 ccaccgggag cgccaacgcg gtgtgcgtat ccgtcgtcgg gatggccagg ttcgtgaaca
     2221 tttccagccg cagccggcca tcccggttgg gtacttcgaa acgaacggcg gcagtacgag
     2281 tctggggatt gaggatgtcg cggatggttg caatgcaccc ctgaatcgtc tggccgggat
     2341 acgcgtcaac tgtgatagag gctgattggc cgatttgaat cttgcccagg tccttttcgt
     2401 aaacctgcgc ttccacgtac acgcgcgaca gatcggcgac cgcgaacaac acgctgccgg
     2461 tatcggccac atctccgggc gctgcttcgg cattgataac gaccccggcg aaaggcgtcc
     2521 ggattgtcgt ggaactcggg tccgcacctg cgggcacgcc gaatcgcttc agacgcatct
     2581 cgatgccggc gatcgcactc tcctgggcac gaatcgcctc ttccaatgcg cgcgcctccg
     2641 tctccgcggc ctctgcttct tttgcgggca cagcaccgat cgcaaccagg ctccggcttc
     2701 tgccggcttg gcggcgagcg tttgcatgct gtattttcag ccgctgcaac tccgcttgtg
     2761 cagtcgcgat ctgagatgac agttcgccgg cttcgatgtt gtccaggtgg gccaatactt
     2821 gatctttccg cacccggtcg cctaccttcg ctacgacctg ttccacacgc cccctggcca
     2881 gcggccgcag atggacaatc cggctctcga tcggttggac tgttcccgtg actcgaactt
     2941 cgtcctgaac ggtgcgcatt cgaactggcg cgacctcgat gccggcacgc ttctgagcat
     3001 ccagactgag ttcgacgacg ttcgcttcct ccaggtactt ttccgggtgc gcctcgtcgt
     3061 ttgtccgctg tccttcagca ggctcctcac gtgcggcatc ttctttcccg gcacaacagg
     3121 caagagaaag tgcaactacc aaaaccagcc atttcattgc agtggacctc ccacggcctt
     3181 ctccagttcg gcaaggcttc ttcgcagttc cgcttgtaga tcaatcgagg tgagctgaag
     3241 atccagcgct cgcctttgct ccgcgaggac atcgagcaga cgcagttgac ccaactggta
     3301 agcttgccga attacatcaa ggttactttc gcttagcgag atggcttgcc catctaaaga
     3361 tgcgagagcc gttcgcgaac tctcaaattg acgccaagcc gcctcgactt caagaggaat
     3421 ggcccgctcc aggtattcca gccggtgttg agccgccctg gtgcgggccg ccgccgcttc
     3481 aatgtttcca agatttcgat ttcgggaaaa caacggaatc gatatgccca cctgcaagag
     3541 gtcatcgcga tcccggagtg gcgtaagctg gttcgcggcg ttgagaccca actgatcgtc
     3601 gaacctggag gtcacgcggc tataactcac cgaagccatc acattcggcc gggcttcggc
     3661 ttccgccagc gaaatctccg cctctgcgaa ttgccgggca aaccgcagca atcgcaaatc
     3721 gggtctctgg ccggccgcct tgctcgccaa tccttccaag gggattgtgg agggaaccgc
     3781 cccggcggcg gcagtccagg tttcaccgga cgcgacccct atgagcctgg agatatcgga
     3841 gcgagccgcc tccatccgcc cggacgctac ggatcgttgc gcttcgac
//
LOCUS       DASTMK010000497         6632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_185110, whole genome shotgun sequence.
ACCESSION   DASTMK010000497 DASTMK010000000
VERSION     DASTMK010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185110"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFQ79_20655"
     CDS             <1..968
                     /locus_tag="VFQ79_20655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142145.1"
                     /translation="SAALFSQTDGFVFPLDIRTYAYHLYDGFSWIRGRNTLKIGGEYR
                     AFGENSDFPTNLKPTFTFFDLMDFADDEVLSVAARVSPTTGRPAGTYRNFRQKEFGIF
                     VQDDWKITRRLTLNLGLRYDNFGPLTEKNSLLSTLVFSDTNLANAGIVQRDPLYERDN
                     NNFAPRVGFAWDPTGNGAWAIRSGFGIFYSRLWSNFTGNTRFNTPYSLPVTLSALSGQ
                     DASGVYRIPFVGDPAFVRPLDDKNGSTAFRPSLQAVDPTIRAPYNMQWTLNIQRRLPA
                     DWRAELAYLGSAGRKLLLRNEINRFSGDGADGVINRVNRSFASIV"
     gene            1041..1754
                     /locus_tag="VFQ79_20660"
     CDS             1041..1754
                     /locus_tag="VFQ79_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142146.1"
                     /translation="MAQVAYTFSRSIDTDSEPFGGGAGELQGSQEVANIRLDRGLSAF
                     DATHRLALNFIWEMPFLRANRGLASSIFGGWQLGGIVSAQSGFPFTVITGVDTNLDGV
                     LTDRPNLVGTSAPLVGSSPKHFSDGAFGDAANWTTLFRPAPAGAVSQLGRNTYRGPSF
                     VTVDASLTKAFSMPWEGSAIEFRAEFFNLTNRVNLRGPSNSLGTFNATTQRWSNVNFG
                     RSTLAFEGRQIQFALKFRF"
     gene            complement(1768..3198)
                     /locus_tag="VFQ79_20665"
     CDS             complement(1768..3198)
                     /locus_tag="VFQ79_20665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0142147.1"
                     /translation="MRRRDLLLAGGAAPFHILKAQRTARPNVLFLISDDQSWLHTSAA
                     GDPVVRTPNFDRIAAQGVRFTHAFCASPSCTPSRAGILTGQHIWRLKESGNLWSTLRR
                     EEYPAYPELLKSAGYHIGSMGKGWGPGDFRAGGWDHNPAGPPFKSFTEFLDQTPAGAP
                     FCFWFGSQDPHRPYEPGTGERSGMNPEHVRVPAWLPDSPEVRRDILDYYFEIQRFDRA
                     VGDMVAALDKSGRLQNTIVVVTSDNGMPFPRAKTNLYDSGTRMPLAVMWPERIPPGRV
                     VDDMVSHTDLAPTFLEAAGIERPRAITGRSQLSILTSRRDGRVENGRNHAVTGRERHT
                     RRRAGGAGYPMRAIRTYDHLYIRNFAPERWPAGDPPDYGDIDTSPSMDFVIANRSSTR
                     FQRYYEMACAKRPAEELYDLKQDPHQLTNLAASAGQEKIGNHLRSQLDRILKDTGDPR
                     LTGSEVLWDSTPYYGGGVLSRPTDER"
     gene            complement(3195..4619)
                     /locus_tag="VFQ79_20670"
     CDS             complement(3195..4619)
                     /locus_tag="VFQ79_20670"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU0142148.1"
                     /translation="MEGLRRREFLGGAASTLLAPTYGNSAPTRPPNIVFAMADDLGYR
                     DLGCYGQKQIRTPNIDRLASEGLRFTDAYAGCTVCAPSRSVLMTGYHMGHTSVRANTG
                     GVPLLAGDVTIGEVLKGAGYKTGVFGKWGLGDIGTDGVPWKQGFDEFFGYLHQVHAHF
                     YYPEFLYKNGERYPLPGNGTGKRTTYSHDAIADQALDFIKRSRNQPFFCYVPFTTPHL
                     ELLVPEDSIDSYRGKFPEKPYRDPRNHYAPQDTPRAAYAAMITRMDRDVGRILALIES
                     LGLDNNTIVFFTSDNGAATPLWKDDYFGSTGGLRGHKQNFYEGGIRTPLVARWPGKIQ
                     AGSETGHLCSFYDVLPTLAELAGIRKIPKTDGLSLAPTLLGRGDQPRHEYLYWELPRY
                     DAKTRKFAKEIPRQALRMGDWKAVRPEPDGPLELYDLETDIAETRNVAAEQPAVMAKV
                     QQFLKEARTEPRPQAQPEPGGWTK"
     gene            4705..>6632
                     /locus_tag="VFQ79_20675"
     CDS             4705..>6632
                     /locus_tag="VFQ79_20675"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEU0142149.1"
                     /translation="MEALFILLLGVIAVRCYLLSRRLKRLLEQSKRNERQIQELTARV
                     YALETGAPAMTVDTPPVEQTALSTEAQPAQDAEPVPADAGLVPEPAPPPRRRTFSGEE
                     WEAVVGGSWLNKAGVLVLVIGIALFLGYSLTNLGAGGRVAVGYGVSVSMLALGIAIER
                     RPPYIVFARGLIGGGWAALYFTTFALHALPAARLVESPLVGALLLCGVAAGMVLHSLR
                     YRSEVVTGLAFFIGFVTLVIAPAPGLSLPASVPLAAALLFVARRFEWTRMAVAGLVFT
                     YSVYVLEAQPEWAMAGHPVLWLYWILFEGFDLLRITSDRRGESIGRTLFPLNACGFLG
                     AALLRARDIDYFLFAAAAAYAVTAIVRAKLRRREAAGAPDQGPFAMVPGTFEAAVTIS
                     AGVLAAAFLQRFTGWRANIALLMEAEFLVLSGLLLRERYLRTLGALVFAVPVLKLLLV
                     DVPKGGEFQTFGPALFNWTPAACIIMAAAFVNRVLTRSGPAYTHAGVLLLALVLAFEL
                     PHAQIGLWWMIAAIPMFAAAERTFVTEFRYDAYALGTLGTIFLWLTQLSRTHPWWPVA
                     AGAALACAATIAAFINRPRSERERQLVDIFTSGAAVLLSATFFWRVLDERAVAPVWAA
                     ICAGLVLAARRINVPKLR"
BASE COUNT         1121 a         2078 c         2027 g         1406 t
ORIGIN      
        1 gaagcgctgc gctcttcagc cagaccgacg gcttcgtctt ccccctagat attcggacct
       61 acgcatatca tctctatgat gggttctcat ggatccgcgg ccgcaacacg ctgaaaattg
      121 gcggtgagta tcgtgctttc ggcgagaaca gcgactttcc gaccaacctt aaaccgacct
      181 tcactttctt cgacctcatg gacttcgccg atgatgaagt cctcagcgtc gcggctcgcg
      241 taagtccgac gacgggccgc cccgcgggca cctaccgcaa cttccgccaa aaggagttcg
      301 ggatcttcgt tcaggacgac tggaagatca cgcgccgcct cacgctgaac ctcggcttgc
      361 gctatgacaa ctttggcccg ctcaccgaaa agaacagtct gctatccacg ctcgtcttct
      421 ccgacaccaa cctggctaac gcaggcatcg tgcagcgcga tcctctatac gagcgcgaca
      481 acaacaactt cgcgccgcga gtaggattcg cctgggaccc caccggcaac ggcgcatggg
      541 ccattcgcag cggcttcggc atcttctaca gccgcctctg gtcgaacttc accggcaaca
      601 cgcgcttcaa caccccctac tctctgcccg tgacattgtc ggcgctgtcg ggtcaggacg
      661 cgtccggtgt ttatcgcatt ccctttgtcg gcgatccggc gttcgtgcgg ccgctcgatg
      721 acaaaaacgg ctccactgcc ttccgcccct cgttacaggc cgtcgatccg accatccgtg
      781 cgccttacaa catgcagtgg acgctcaata tccagcgccg cctgcctgcg gactggcgcg
      841 ccgagttggc ctatctcggc agcgcgggcc gcaagcttct gcttcgcaat gaaatcaacc
      901 gcttctccgg cgacggtgcg gatggcgtga tcaaccgcgt caatcggagc ttcgcctcca
      961 tcgtataggg ctcgaacggt gtttcttcga tataccacgc gttcaccgcg agcgctgtca
     1021 aacgcttctc gcactcctat atggcgcagg tggcctacac cttctcccgc tctatcgaca
     1081 ccgactcgga accgttcggg ggcggggctg gcgagcttca gggtagccag gaagtagcga
     1141 atatccggct ggaccggggc ctgtccgctt tcgacgcaac gcaccggctg gcgctgaact
     1201 tcatctggga aatgcctttc ctgcgtgcaa accgcggcct cgcgagctcg attttcggtg
     1261 gatggcagct cggagggatt gtgtcggcgc agtcgggatt cccgttcacg gtcatcacgg
     1321 gcgtggatac gaacctcgat ggcgtattaa cggaccggcc caatcttgtt ggaacaagcg
     1381 ctcctcttgt gggcagttcg ccaaaacatt tctccgacgg cgcgtttggc gatgccgcca
     1441 actggaccac ccttttcaga cccgctccgg ccggcgccgt ttcccagctc ggcaggaata
     1501 cttatcgcgg tccctctttc gtaaccgtcg acgcgtctct gacgaaggcg ttcagcatgc
     1561 cgtgggaagg cagcgcgatc gagtttcgcg ccgagttttt taacctgacc aaccgcgtga
     1621 atcttcgcgg tccgtcgaat tctctcggca ccttcaacgc cacaacgcaa cgctggtcga
     1681 acgtaaactt tggccgctct acactggcct tcgaaggtcg tcagatccag tttgcgctca
     1741 agttccggtt ttgagcgtgt gcgcaggtta gcgttcatcc gttggacggc tcagaacccc
     1801 gccgccgtaa tagggggttg aatcccagag gacttcgctg cccgtcagcc ggggatcgcc
     1861 tgtatctttc aggatgcggt ccaactggga tcgtaaatga ttcccgattt tctcctgtcc
     1921 ggcgctggcc gcaaggttgg taagctgatg cgggtcctgc ttcaggtcat acaattcctc
     1981 ggccggccgc tttgcgcacg ccatttcgta atagcgctgg aaccgggtgg aggaccggtt
     2041 tgcgatcaca aaatccatgg aaggcgaagt atcgatatct ccgtagtcgg gcgggtcgcc
     2101 ggcgggccag cgctccggag cgaagttgcg gatgtagaga tgatcgtagg tccggatggc
     2161 gcgcatggga tatccagctc caccggcgcg gcgccttgtg tgccgctcgc ggcccgtgac
     2221 ggcatggttg cgcccgttct cgaccctgcc atcccttcgc gatgtcagaa tgctcagctg
     2281 gctgcgcccg gttatcgcgc gtgggcgttc aatacccgca gcctcgagga acgtcggggc
     2341 gagatcggta tggctcacca tgtcgtccac cacccgcccc ggcggtatcc gttccggcca
     2401 catgacagct aagggcatgc gcgtgccgga gtcgtagaga ttcgtcttgg cgcgtggaaa
     2461 cggcatgccg ttgtcgctgg tgactaccac gatggtgttc tgaaggcggc ccgatttatc
     2521 caaagcggcc accatgtctc ccacggcccg gtcgaaccgc tggatctcga aataataatc
     2581 caggatgtcc cggcggactt ccggtgaatc cggaagccag gcgggaacgc gaacatgctc
     2641 cggattcatg ccggagcgct caccggtccc cggctcatag ggacgatgcg ggtcctggct
     2701 gccgaaccag aagcaaaacg gggcgccggc gggagtctgg tcgagaaatt cggtgaacga
     2761 tttgaagggt gggcctgcag ggttgtgatc ccatccgccg gcgcgaaagt cgcctggccc
     2821 ccatcctttc cccatcgatc cgatgtggta gcccgcgctc ttcaacagtt ccggatacgc
     2881 cggatattcc tcgcgccgga gcgtgctcca cagattgccg ctctctttga gccgccagat
     2941 atgctgcccg gttaaaatgc cggcgcgcga cggcgtgcag gaaggagacg cgcagaaggc
     3001 atgcgtgaag cgcacacctt gcgctgcaat ccggtcgaag ttcggcgttc tcacaacggg
     3061 gtcgccggcc gcgctggtgt gcagccagga ctggtcgtcc gagatcagaa acagcacgtt
     3121 gggacgcgcc gtccgttgcg ctttgaggat gtggaacggc gccgccccgc ctgccagcaa
     3181 cagatcccgt cttctcattt ggtccacccc ccaggttccg gctgtgcctg cggccggggc
     3241 tcggtgcgtg cttctttcag gaactgctgg accttggcca tcaccgcggg ctgctccgct
     3301 gccacgttcc gcgtctccgc gatatcggtt tccaggtcgt acaattccag gggcccgtcc
     3361 ggttccgggc ggaccgcctt ccaatcgccc atgcgtaagg cttgcctcgg aatctctttc
     3421 gcgaacttcc gcgtcttggc gtcgtagcgg ggcaactccc agtagagata ctcgtgccgt
     3481 ggctggtctc cacgcccgag cagcgtaggc gccagagaca agccatctgt cttcgggatt
     3541 ttccggatac cggcaagttc cgccagcgtg ggcagaacgt cgtagaacga acagaggtgt
     3601 ccggtctcgc tcccggcctg gatcttgccg ggccaacggg ccacgagcgg cgtacggata
     3661 ccgccttcat agaagttctg cttgtggccg cgcaaaccgc cggtgctgcc gaagtaatcg
     3721 tctttccaga ggggtgtcgc cgcgccgttg tcgctggtaa agaacacgat cgtgttgttg
     3781 tcgagcccca gcgactcgat cagcgccagg atgcgtccca catcgcggtc catgcgcgtg
     3841 atcatcgccg cgtaagcggc acggggagta tcctgtggag cgtagtggtt gcgcggatcg
     3901 cggtacggct tttccgggaa cttgccccgg taggaatcga tagaatcttc cggtaccagc
     3961 agctccaggt gtggggtagt gaaaggcaca tagcaaaaaa acggctggtt gcgactccgt
     4021 ttgatgaagt cgagagcttg gtccgcgatc gcgtcgtgcg aatacgtcgt gcgcttgccg
     4081 gttccgtttc caggcagcgg atatctttca ccgtttttgt agaggaattc cgggtagtaa
     4141 aagtgcgcgt gcacctggtg cagatacccg aagaattcat caaagccctg tttccaggga
     4201 acgccgtcgg tgccgatgtc gcccagaccc cactttccga acacgccggt tttgtagccg
     4261 gcgcctttca gcacttcgcc gatcgtgaca tcgccggcca gcagcggcac gccacccgtg
     4321 ttggcgcgca cggacgtatg ccccatgtga taaccggtca tcagcacact ccgcgatggc
     4381 gcgcagacgg tgcagccggc ataagcatcg gtgaaccgca agccctcgga cgccagccgg
     4441 tctatgttcg gtgtgcgaat ctgcttttgc ccgtagcagc cgagatcgcg gtagcccaga
     4501 tcgtcggcca tcgcgaaaac aatattcggc ggccgcgtgg gagcactgtt gccgtatgtg
     4561 ggagcgagca gcgtgctggc tgcaccgccg agaaattctc tgcgcctgag accttccata
     4621 tctgccctga acgtatcagc atccggcgga tcgggtcaaa cccggccgga gccgctttcg
     4681 aaaatggtaa tctggtgacc gcgaatggaa gcccttttca ttctgctgct gggcgtgatc
     4741 gcggtgcgct gttatttact gagccgccgc ttgaagcggc tgctggaaca atcgaagcgg
     4801 aacgagcgcc agattcagga attgaccgcc cgcgtgtacg cgttggagac aggcgctcca
     4861 gcaatgacgg ttgacacgcc gccggttgag cagactgcac tttcgacaga ggcgcaacct
     4921 gcgcaagacg cagaacccgt ccccgccgat gccggattgg tccccgagcc ggcgccaccg
     4981 ccgcggcgcc gtactttcag tggcgaggaa tgggaagccg ttgtcggcgg aagctggctc
     5041 aacaaagccg gcgttctggt gctggtcatc ggcatcgcgt tgtttctcgg ttattcgttg
     5101 acgaaccttg gcgcgggcgg gcgcgtcgcc gtcggatacg gggtgagcgt atccatgctc
     5161 gccctgggca tcgcgataga gcgccgcccg ccatacatcg ttttcgcacg aggtctgatc
     5221 ggcggcggat gggcggcgct ctatttcact acctttgcac tgcatgcgtt gccggctgca
     5281 cgcctggtgg aaagtcctct cgttggtgcg ctgctgctat gcggtgtggc cgcgggaatg
     5341 gttctgcatt cactgcgcta ccgctccgaa gtagttaccg gactggcttt cttcatcggt
     5401 ttcgtcacgc tggtgatcgc tccggcgccg ggcctttcgc ttccggcttc cgtgccactg
     5461 gcggcggctt tgctgtttgt ggccaggcgg tttgaatgga cccgaatggc tgtcgccggg
     5521 ctcgtcttca cctatagcgt ttacgtcctc gaggcacagc cggaatgggc catggcgggc
     5581 cacccggtgc tctggctgta ctggattttg tttgaaggct tcgacctgct gaggatcacc
     5641 agtgaccggc ggggcgaaag cattgggcgc acccttttcc ccctgaacgc ctgcggattt
     5701 ctgggcgcgg cgttgttgcg cgcgcgggat atcgattact tccttttcgc cgccgcggcg
     5761 gcgtacgccg taaccgccat cgtgcgtgcc aagttgcgcc gccgcgaggc ggccggtgcg
     5821 ccggaccagg gtcctttcgc aatggtcccg ggaacgttcg aagcagcggt aaccatctcc
     5881 gccggagttc tggccgccgc gtttttacag cgtttcaccg gttggcgcgc gaacatcgca
     5941 ctgctgatgg aagcggagtt cctggtgtta tcgggcttgc tgcttcgcga gcgctacttg
     6001 cgaacgctgg gcgcgctggt gttcgcagtg ccggtattga agctgttgct ggtggatgtt
     6061 cccaagggcg gcgaatttca aactttcggc cccgcgctct tcaactggac cccggcggct
     6121 tgcatcatca tggccgcggc atttgtgaac cgtgtcctca cccggtccgg accggcatac
     6181 actcacgccg gagttctatt gcttgcgctg gtgctggctt ttgagctgcc gcacgcacag
     6241 attggcctgt ggtggatgat tgccgccatt cccatgttcg ccgccgccga gagaacgttc
     6301 gtaacggaat tccgttacga cgcctatgcg ctcggtacgc ttggaaccat cttcctgtgg
     6361 ctgacacaac tttcccggac ccacccctgg tggccggtcg ccgccggcgc cgctctcgcg
     6421 tgcgccgcaa ccatcgcggc ttttatcaac cggccgcgct ccgaacggga acggcagctt
     6481 gtggacattt tcacttcggg cgccgccgtt ctgctatcgg ccacgttctt ctggcgcgtg
     6541 ttggatgagc gcgccgtcgc gccggtttgg gcggcgattt gcgccggcct ggtcctggca
     6601 gcgaggcgca taaacgtccc caaattgcgg tg
//
LOCUS       DASTMK010000498         5392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_313984, whole genome shotgun sequence.
ACCESSION   DASTMK010000498 DASTMK010000000
VERSION     DASTMK010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313984"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFQ79_20680"
     CDS             <1..489
                     /locus_tag="VFQ79_20680"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0142150.1"
                     /translation="VNQPRRGMFVVSLSEEELQKINSLRLVLEAEALRLCSARSTPQN
                     LRKLEQLADRMERSTGTTALEATRMDLEFHRAMWSFSGNEFLEKTLTSVTAPLFAYAV
                     LTKPKAEKMRMILDSHRPLVEFVAGTSRRTADDVIFDHLSLRWAEPDKYSSRRVRNGG
                     AS"
     gene            complement(493..1428)
                     /locus_tag="VFQ79_20685"
     CDS             complement(493..1428)
                     /locus_tag="VFQ79_20685"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU0142151.1"
                     /translation="MRSAGWFLAAIVPALAIANWRSGTPMPQPRAGYIAGAAGGKLII
                     AGGSYWESGRKIWSARTDLYDAATGMWTAGPPLPVPRSDAACLVWNSALYAWGGGAEG
                     SVSAECLRLENGKWQTVPAMNLPEPRLYGVASELGRRIYVFGGIREAGKHETAANTLW
                     RWVSERRKWEARASLPGPPRSLNGFAASGGKLYVLGGYTVQNGAAANLDDVWEYDPAG
                     DQWSPAGRLPVARRAWWALAGPTGSVLLFGGYTDSFSAEIWSYDPRTATSTVAGSLPI
                     ALADTKFFRIGREFVGAGGESGPHVRSRATLQGAF"
     gene            complement(1425..2384)
                     /locus_tag="VFQ79_20690"
     CDS             complement(1425..2384)
                     /locus_tag="VFQ79_20690"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU0142152.1"
                     /translation="MHPDNFRLEDFPRASRHSLEFQFTAGGQEIGFPVLVVRGDESGP
                     LLLVSAGVHGDEYEGVRAILEIFAELSPASMKGDFVAVPALNAPAVWSCTRTSPLDGA
                     NLARVFPGRADGSPTEAIAWYFDQTLLPLADLYADLHSAGVACEMPLLVGYDATDARS
                     RAAAFAFGAPVIWAHPNVPPGRTVSAAKARNVPSIYTEARGAGRIHPDDLSIYKQGLR
                     NLLAHMGITSEPPRAAPSPLHLLGDGNIDQSILAGHRGFLIPHTSILGATEKGQKLGD
                     LVDLTGNVLETYTAPASGRVVLIHACPRVLPGEPVFLLAGVHP"
     gene            complement(2388..3476)
                     /locus_tag="VFQ79_20695"
     CDS             complement(2388..3476)
                     /locus_tag="VFQ79_20695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0142153.1"
                     /translation="MRFLAFALPALAFAASLETGTVVKSDGGPLHRNTIPQVARLGDG
                     RLLCVFGAYAKDSGDGRIMGTISADGGRTWSTPRLLIDDPGKNDGDPNILVDGARVFV
                     YGTRVNIPNRIDKAWTYVVRSEDNGATWSAPQEVQIPRQYTPGKQHNGIQLLDGTYAM
                     GISWDLWAEKGMAARTEGEMVLASGLLKSIDGLRWTLHGSITTFVPKQTPGSTNGLCE
                     PSIVQFAGGSILMLLRSGDSRHWQSRSEDSGVTWSHPEPSALVGHNTPTALWRLEGPA
                     DEIVAVWNNSPVTRFPLTVAISSDKGETFSTPRILANPIGYQVSYPGVTQTKDGTIVA
                     VWQQQLPDGGRDIRFARFSREWVLGSGQ"
     gene            4059..>5392
                     /locus_tag="VFQ79_20700"
     CDS             4059..>5392
                     /locus_tag="VFQ79_20700"
                     /inference="COORDINATES: protein motif:HMM:NF014853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEU0142154.1"
                     /translation="MTGPFGPPSLLGDPSRKGDLARKIPVPSSSCGVPAAAAYSLNFV
                     VVPPAGAGVAWLAAWPDGAPWPGTVILNAPAGGVVGASAVVASGPEGAIQVFSTDPTD
                     LVIDVNGYYVRQPGIQFQGQWNFATTYAKGDAVFYDGSSYIGLSDGNAGNAPGNGVAW
                     ALLAQQGATGRSGPQGANGPAGPAGPAGSAGPIGPQGLIGLAGPTGATGATGPAGPPV
                     SFQGTWNPATTYATGDAVSYNGSSYISLSGGNIGNTPAGGAPWAILVQQGATGATGPA
                     GPVGATGPAGPVGPIGPIGPAGPAGATGATGATGPAGPPVSFQGTWNPVTTYATGDAV
                     FHDRSSYISLSNGNVGNTPTGGAPWALLAQQGATGPAGPTGPAGPTGATGATGATGLA
                     GPAGATGPAGPTGPTGPQGPPGSGGGIALLARTNLTGTNDTVYAYPAGFAAQ"
BASE COUNT          981 a         1703 c         1702 g         1006 t
ORIGIN      
        1 gtgaaccagc cgcgccgcgg gatgtttgtc gtcagcctga gtgaagagga gttgcagaag
       61 atcaacagcc tccggctggt gttggaggcc gaagcgctgc gtctgtgctc cgcgcgctcg
      121 acgccgcaga atcttcgcaa gctggagcaa ctggccgaca ggatggaacg cagcacaggc
      181 accaccgcgc tcgaagcgac gcgaatggat ctcgagtttc accgcgcgat gtggagtttc
      241 agcggaaatg agtttctcga gaagactcta acgagcgtaa ccgcgccact gttcgcctac
      301 gccgtgctca ccaagccgaa agcggagaag atgcgaatga tcctcgattc gcaccgcccg
      361 ctggtggagt ttgtggccgg tacctccagg cgcaccgccg acgacgtgat cttcgatcat
      421 ttgtcgctgc gctgggccga gccggacaaa tactcctccc gccgcgttcg taatggaggc
      481 gcatcctgaa aatcaaaacg ctccctggag cgtcgcgcga gagcgaacgt gcggtccgga
      541 ctctccgccc gcgcctacga attcccggcc gatacggaaa aatttggtgt cggccagcgc
      601 gatgggaagg ctgccggcaa cggttgatgt agcggtcctc gggtcgtaag accagatctc
      661 ggcggagaac gaatcggtgt agccgccgaa caatagaacg cttcccgttg ggccggccag
      721 cgcccaccac gcccggcgag caaccgggag gcggccggcc ggactccatt ggtcgcccgc
      781 gggatcgtat tcccagacat cgtccagatt cgcggccgcg ccgttttgga cggtatagcc
      841 gcctaatacg tacagctttc ctccgctggc ggcgaacccg ttcagacttc tcggcggtcc
      901 tggcagcgaa gcgcgcgctt cccatttccg ccgctcgcta acccatctcc acaaagtatt
      961 cgccgccgtc tcatgtttgc ccgcttcgcg aatgccgccg aagacgtaaa tccggcggcc
     1021 gagctccgat gccacgccgt agaggcgcgg ttcgggaaga ttcatggcgg gcacggtttg
     1081 ccattttccg ttttcgagcc tcaggcactc ggcactcacc gagccctccg cgccgcctcc
     1141 ccacgcatac agtgcgctat tccacaccag acaggccgcg tcgcttctgg gcacgggcag
     1201 cggagggcct gcggtccaca ttccggtggc agcgtcgtat agatctgtac gggcgctcca
     1261 gatcttcctg ccgctctccc agtagcttcc gccggcgata atcaacttgc cgccggcggc
     1321 gcccgcgatg tagccggcgc ggggctgggg catgggcgtg ccgctcctcc agttcgcaat
     1381 cgccaaagcc ggcacgatag ccgcaaggaa ccaaccggcg cttctcacgg atgcactccg
     1441 gccagcagga acaccggttc gccgggcaat acgcggggac aggcatgaat gagcaccaca
     1501 cggccgcttg ccggggccgt ataggtctcc agcacgttgc cggtcaagtc gaccaggtcc
     1561 ccgagcttct gccctttttc ggtcgcgccg agaatactgg tgtgcggaat cagaaacccg
     1621 cgatggccgg cgagaatgct ctggtcgatg ttgccgtcgc ccaaaaggtg cagaggagat
     1681 ggagcagccc gcggaggttc ggaggtgatg cccatatgcg ccagcaggtt tcgcagcccc
     1741 tgtttgtaga tcgaaagatc gtccggatgg atgcggccgg cgccgcgcgc ttcggtatag
     1801 atcgacggca cgttgcgagc cttcgccgcc gatacggtgc ggcccggcgg cacattggga
     1861 tgcgcccaga tcaccggcgc gccgaaagcg aaggcggcgg cgcgcgatct cgcgtccgtc
     1921 gcgtcatatc caaccagcag cggcatctcg caggcgactc cggcgctgtg caggtcggca
     1981 tacaggtcgg ccagcggcag caacgtttga tcgaagtacc aggcgatggc ctcggttggg
     2041 gagccgtccg cccggcccgg aaacacccgc gcgagattcg cgccgtccag cgggctggtt
     2101 cgcgtacagc tccaaacggc cggtgcgttc agtgcgggca cggcaacgaa gtcccccttc
     2161 atgctcgcgg gagacagttc cgcaaagatt tccagaattg cgcgtacgcc ttcatattcg
     2221 tctccatgca cgcccgccga caccagcagt agcggaccgc tctcatcgcc gcggaccacc
     2281 aagacgggga agccgatctc ctgcccgccc gctgtaaact gaaactccag gctgtggcgg
     2341 gaagcgcgtg gaaaatcctc caggcggaag ttgtcgggat gcatggctta ttgcccggag
     2401 cccagaaccc actcgcgcga gaaccgggca aaacggatgt cccggccgcc atccggcaat
     2461 tgctgctgcc acaccgcgac gatggttccg tctttcgtct gcgtcacgcc cggataggaa
     2521 acctggtagc cgatggggtt ggccaggatg cgcggtgttg agaacgtctc gcctttgtcg
     2581 ctgctgatcg cgacggtcaa cggaaaacgt gttaccggag aattgttcca taccgcgacg
     2641 atttcgtccg ccggcccttc caggcgccag agtgcggttg gagtgttgtg gcccaccaat
     2701 gcgcttggtt cgggatgcga ccaggtgacg ccgctgtcct cactacggct ctgccagtgg
     2761 cgcgaatctc ccgagcgcag aagcatgagt atgctgccgc cggcgaattg cacgatggag
     2821 ggttcgcaca agccgttcgt cgatcccggc gtctgtttcg gaacgaacgt ggtaatgctg
     2881 ccatgcagcg tccagcgcaa cccgtcgatg gattttagca gcccgctcgc cagcaccatc
     2941 tcgccttccg tgcgcgccgc catgcccttt tccgcccaca ggtcccacga gattcccatc
     3001 gcgtaggttc catccaggag ctggatgccg ttgtgctgct tgcctggagt gtactgtctc
     3061 ggaatctgca cctcctgggg cgcggaccac gtcgcgccgt tgtcttcgct gcgcacaaca
     3121 tacgtccatg ctttatcgat gcgattcgga atattcacgc gcgtgccgta cacgaacacg
     3181 cgtgcgccgt ccaccagaat gttggggtcg ccgtcgttct tcccggggtc gtcgataagc
     3241 aaccgcggcg tggaccacgt gcgtccgcca tccgctgata tcgttcccat gatccggccg
     3301 tcgccgctgt ccttcgcata agctccaaag acgcacagca ggcgcccgtc ccccagccgc
     3361 gcaacctggg gaatggtgtt gcggtgcaat gggccgccat cggatttcac cacggtcccg
     3421 gtttccaggg aagctgcgaa tgccagggca ggcagagcga atgcgagaaa gcgcatgggc
     3481 cgagtctata aaactgtcga cagattgtaa agagcggcgt ccgtgtgccg cggtacgacc
     3541 ggtaacagag tgtatactgg aaaaactgcc gcgggccctc cggcccctcc ggtattgact
     3601 gggcattgca agagcgaaag cagatttgtt ctctgcttcc gaactggata ttatcaatct
     3661 gcaccgatat accgcgcatt ttgtatccga tgctattcgg ttacgaattt tctgacagga
     3721 tttgtcagaa ataggcaccg ctttctgcat tcgggcggcg ggctttcttg tgacagcgcc
     3781 catcaggggc gcggaagggg tctctcttgt ccaaggcaat aatcccggcc ttgatagggc
     3841 ttctggtttg ctcaggcgtc tgtgccgcat tacaggcgca agtgcctctt aatccccctt
     3901 tgaaaaattg ggaggcgact gcggtatggg cgcctgcttc ctctccttcc tctaacgcga
     3961 aaagggccga gcgcttcgcc agcgaagatg taggaactcc gctgacattc gtcgcgatca
     4021 caccgtgccg gcttttggat acacggggtt cttccggaat gaccgggcct tttggcccgc
     4081 cctccctgct tggcgatccg tcgcggaaag gggatcttgc gcgcaagatc cctgtaccca
     4141 gctcaagctg tggcgttccc gccgcggcgg cttactcttt gaattttgtg gttgttcctc
     4201 cggccggagc gggagtggcg tggcttgctg cctggcctga tggggcgccc tggccgggaa
     4261 cggttatttt gaacgcgccg gcaggtggcg tggtgggggc ttccgccgta gtagccagtg
     4321 ggcccgaagg tgccattcag gtgttttcca cggacccgac cgacctggtg atcgatgtca
     4381 acggatatta tgtgcggcaa cccggaatcc aattccaggg gcagtggaac tttgcaacca
     4441 cctatgccaa gggcgacgcg gtgttctatg acggatccag ttacatcggt ttgtccgacg
     4501 gcaatgccgg caatgcgcca ggcaatggcg tggcgtgggc gttgctggcg cagcagggag
     4561 caacgggccg gtccgggccg cagggagcta acgggcccgc gggtccagcc ggtccggcag
     4621 gttctgcggg tcccatcggt ccgcaaggtc tgatcggcct ggcaggtcct acaggagcaa
     4681 caggagcaac aggcccggca ggcccgcctg tttctttcca gggcacgtgg aatcccgcaa
     4741 caacgtatgc cactggcgat gcggtgtcct ataacggatc gagctacatc agcttgtccg
     4801 gcgggaacat cggtaacacg cctgccggcg gtgcgccttg ggccatattg gtgcaacagg
     4861 gagcgaccgg agcaaccggt ccagcagggc cggtgggagc aacgggtccg gcaggtcctg
     4921 tgggtcccat cggtccgatc ggtccggcag gtcctgcggg agcaacaggt gcaacaggag
     4981 caacgggtcc ggcaggtccg cctgtttcat ttcaggggac gtggaatccc gtaacaacgt
     5041 acgccaccgg cgacgcggtg ttccacgatc gatcgagcta catcagtttg tccaatggaa
     5101 atgtcggcaa cacgcccacc ggcggcgcgc cttgggcgct cctcgcccag cagggtgcaa
     5161 cgggtccggc aggtccgacc ggccctgctg gtccaaccgg cgcaacggga gcgactggag
     5221 caaccggcct tgccggtcct gcgggcgcga caggtccagc cggaccaacg ggtcccaccg
     5281 gaccgcaggg gccccccggt tccggcggag gaatcgcatt gctcgccaga acgaatctga
     5341 cgggtacgaa cgacacggtc tacgcgtatc ctgccggatt cgccgcccaa ca
//
LOCUS       DASTMK010000499         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_185218, whole genome shotgun sequence.
ACCESSION   DASTMK010000499 DASTMK010000000
VERSION     DASTMK010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185218"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFQ79_20705"
     CDS             <1..304
                     /locus_tag="VFQ79_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0142155.1"
                     /translation="TEKSVVLQLDVGTCLEVGSDPVAWIRKNPGRIASIHLKDWSPDR
                     GYKVLFGEGAAPWKKIFEAAEQTGGVEQYLIEQEGSDYPPMETADRCLANFRKLRG"
     gene            complement(306..1418)
                     /locus_tag="VFQ79_20710"
     CDS             complement(306..1418)
                     /locus_tag="VFQ79_20710"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0142156.1"
                     /translation="MRRRDIVLGMGSIMSGRAAEHPMLEQGISLLRQSADAGEVHAAT
                     LYTGMAGRTHRFGFGRARSPEEVFLIASITKPMTAAGVMVLADRGELALDDPVQKFIP
                     EFSEGDRKLVTMRHLLTHSSGLPDMLPENVELRRRQAPLQEFVKGAIRTPLLFKPGTR
                     VQYQSMGILLAAEVARRITGQPFPEFLDSQVFGPLGMKHTALGLGKFRIEDAARCQVE
                     NAEPLYGGGSKDTAKWDWNSPYWRNLAAPWGGAHSNGPDIARFLGYFLEPDGRVLKPA
                     TAAAMIVNQTEGLNEPRGIGFVVKPGSFGSRCSIKTFGHGGSTGTLAWADPQTKTVCV
                     VLTTLPARVSNAKLLKPVSDRVSEWAAAPRSMPLHR"
     gene            complement(1476..>2781)
                     /locus_tag="VFQ79_20715"
     CDS             complement(1476..>2781)
                     /locus_tag="VFQ79_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU0142157.1"
                     /translation="APPAGQLAVLAIDPGIRTGCKIAVVDETGKFLEHGVIYPHQPKN
                     DTAGAARTLHSLITRHNVKAIAIGNGTASRETDAFVREFLQQHDLNKVFSVMVNESGA
                     SVYSASDIARQEFPDLDLTIRGAISIARRLQDPLSELVKIDPKSIGVGQYQHDVDQRQ
                     LQYSLETVIESCVNRVGVDMNTASWALLRYVAGINERTARKIVEYRNDNGIFRSRVQL
                     TAVPGVGPKTFEQAAGFLRIRGGGHPLDMTAVHPESYAIVEQMAASLGMTIEELIRNP
                     GALEKVNRDELSAGVYTLNDILEELRKPGRDPREQFVAPKFREEVREIADLKPGMVLE
                     GAVTNVTRFGAFVDIGVHQDGLVHVSELSNRFVQDPGEVVKAGQIVKVQVLSADPKTK
                     RIALSMKALQAPPANPQRRPGNKPQPTLDAKLAALSDRWKTR"
BASE COUNT          536 a          857 c          837 g          551 t
ORIGIN      
        1 cacggagaag agtgtcgtat tgcaacttga cgtcggcacg tgtctggaag tgggctccga
       61 cccggtcgcc tggattcgga aaaaccccgg ccgcatcgcc tccatacacc tgaaagactg
      121 gtctccggac cggggctaca aagttctatt cggcgaaggt gcggcgccgt ggaagaagat
      181 tttcgaggcg gccgaacaaa ccggtggtgt cgagcagtac ctgatcgagc aggaaggaag
      241 cgactacccg cccatggaga cagcggatcg ctgcctggca aacttccgta aactgcgcgg
      301 ctgatttaac gatgcagcgg catggaacgg ggtgcggccg cccattcgga gacccggtct
      361 gaaacgggtt tcagcagctt tgcattggag acgcgcgcgg gaagcgtcgt gagcaccaca
      421 cagactgttt tagtctgagg gtccgcccag gcaagggttc ctgtcgatcc tccgtggccg
      481 aatgtcttga tggagcaccg gctgccgaag cttcccggct tgacgacgaa gccgatgccg
      541 cgtggctcgt tgaggccttc ggtctgattc acgatcatcg ccgccgccgt ggcgggcttc
      601 aacacgcggc cgtccggttc cagaaagtaa ccgagaaacc tcgcgatatc gggaccgttc
      661 gaatgagcgc caccccaggg cgcagccagg ttgcgccagt acgggctgtt ccaatcccac
      721 ttcgcggtat ctttcgaccc gccgccgtac aacggttctg cgttttccac ctggcagcgc
      781 gcggcatctt caatccggaa cttccccagc ccgagtgccg tgtgcttcat gcccagcggg
      841 ccgaacacct gtgaatcgag aaattccgga aacggctgac ccgtgattcg ccgggcaact
      901 tcggccgcca gcaatatgcc catgctctga tactgaacac gcgtgccggg tttgaacagc
      961 agcggtgtcc ggatggcgcc tttaacgaac tcctggagcg gcgcctggcg ccggcgcaac
     1021 tccacattct ccggaagcat gtcgggcagc cccgaggaat gcgtgagcag gtgccgcatg
     1081 gtaaccagct tccggtcgcc ttcggagaac tccggaatga acttttgaac cgggtcgtcg
     1141 agcgccaact cgccgcggtc ggccagcacc atcacgcccg ccgcggtcat cggcttggtg
     1201 atggaagcga tcaggaaaac ttcttccggc gagcgtgccc ggccaaaacc gaaccggtgg
     1261 gtccggcccg ccatgccggt gtacaaggta gcggcgtgaa cctcgccggc gtccgcggat
     1321 tgccgcaaca gacttatgcc ctgttcaagc atggggtgtt ccgcagcccg tccgctcatg
     1381 atggatccca tgccgagaac gatatcccgt cttcgcatca ggtcgcctcc aggagtgata
     1441 gacgatccta tcaccaaccg ggttgcggac gtttcctaac gcgttttcca gcgatcggag
     1501 agtgccgcca gcttggcatc cagggtgggc tgtggtttgt tgccgggacg ccgctgggga
     1561 ttggcggggg gcgcctgaag cgccttcatc gatagcgcaa tgcgcttcgt cttcgggtcc
     1621 gcgctcaaga cctggacctt tacaatctga ccggccttca ccacttcccc tggatcctga
     1681 acgaagcgat tcgatagctc gctgacgtgc accaggccat cctgatggac gccaatgtcg
     1741 acaaacgcgc cgaagcgcgt gacgttcgtc accgcgccct ccagcaccat gccgggcttc
     1801 aggtccgcga tttcccttac ttcttcgcgg aatttcggag caacgaactg ttcgcgcggg
     1861 tcgcggccgg gcttgcgcag ctcttccaga atgtcgttca aggtgtaaac acctgccgaa
     1921 agctcatcgc ggttcacttt ttcgagggcg cccggattcc ggatcagttc ctcgattgtc
     1981 atgccgagcg atgccgccat ctgctcgaca atcgcataag attccggatg cacagccgtc
     2041 atatcgagtg gatgaccgcc gccccggatc cggagaaacc cggccgcctg ctcgaaggtc
     2101 ttcggcccga ctccaggcac agcggtcaac tgaacgcgtg aacggaatat cccgttatcg
     2161 ttccggtact cgacaatctt gcgtgcggtt cgttcgttga tgcccgccac gtagcgcagc
     2221 agcgcccagg aagcggtatt catatcgacg ccgacgcggt tcacacagct ttcgatcacc
     2281 gtttcgagcg agtactgcaa ctgccgctga tcgacgtcgt gttggtattg gccgaccccg
     2341 atggatttcg gatcgatctt gaccagctcc gacaacgggt cctgtaagcg gcgagcgatg
     2401 gagatggcgc cgcgaatcgt cagatccaaa tccggaaact cctggcgagc gatatcggag
     2461 gcggaataca ccgatgctcc ggattcgttt accatcacgc tgaaaacttt attgaggtca
     2521 tgctgctgga ggaactcgcg cacgaaggcg tctgtctcac gcgatgccgt accgttgccg
     2581 atcgcaatcg ctttcacgtt atgcctggtg atgagcgagt gcaaagtgcg ggccgcgccg
     2641 gccgtatcgt ttttcggctg atgcgggtag atgacgccgt gctccaggaa tttgcccgtc
     2701 tcatccacta cagcgatctt gcaaccggtg cgaatgccag ggtcgatagc gagtaccgca
     2761 agctggcctg caggaggcgc a
//
LOCUS       DASTMK010000500         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_152653, whole genome shotgun sequence.
ACCESSION   DASTMK010000500 DASTMK010000000
VERSION     DASTMK010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152653"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFQ79_20720"
     CDS             <1..1215
                     /locus_tag="VFQ79_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142158.1"
                     /translation="VKPPLDDEQVEKTLDRILSSPAFRNSKQCQKFLRYVVNHSHAGE
                     EDRLKERSIGIEVFGRDPGYDTAGDPIVRVRATEVRKRLAQYYQESAVEGDVRFEIPA
                     GSYKAEFHPVAVRPAVMEGLPPRPRRRIWRPWLLVPALAALLLVAGYFALRPSETADI
                     LDQFWAPVIRNPKPVLLYCGQPVVYFLSREVRDRYGKEPADRQSASQPVALPPDAVIR
                     GRDVIPVTEQFVGIGNAHTAAQLTSLFAARHKPIEIRYANDLSFSDLRGSPSVLIGAF
                     SNLWTLEMTGKLRFVFEQEKGARWIRDRSKNRTWQLDRLASDGKTPEDFAIVSRLFDS
                     ATGQLLISAAGITQYGTRSAGEFLTDSSRMREALAKAPPDWAKQNMQVLLHTTIYRGT
                     PAPPRVLDVHFW"
     gene            1406..>3981
                     /locus_tag="VFQ79_20725"
     CDS             1406..>3981
                     /locus_tag="VFQ79_20725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142159.1"
                     /translation="MRHCILGITAGILLMAANFDLFAQTTLGNILGAATDPTGAAVPG
                     VSVTVRNLGTGIAHQTVTRESGLYEVTHLIPGRYSVSAERPGFKSIQVTNVFLETSAA
                     VRVDLPLEIGEVTTSVTVEAAAAPINTESADVAAIRGNDVMVKLPINNRNSFYFTMLV
                     LTPGAIRGQGSNVSLGGARGFQWHSTVDGTSTRSPLFANAVGPAESGMEMTAELRIQL
                     ANDKAESALPGGYYATSKSGTNEYHGSVFYYHSNSRLTARNTFSTSVPFQVQNNYGVA
                     GGGPIVKNRTFFFGVYERFPSRSERIFSNTNVPTAAFRRGDFSSLLPGSVIRDPLTNL
                     PFPNNVIPSDRISSVTQSVQEHFFPLPNFGPENTFLRNWRGVGPGKSFKSMVEVRVDH
                     RLSAANSLFGRFSWNTTGANVYDYDLPTMPIRDQDRRTIAFSISDTHIINPSTVNEFR
                     FGVMRSRNPAFNPLDGPALVEKFGLEGIAWNPEIATGAPVFSFNNFSQIGATDLYQDP
                     SERIYQISNNLTWTRSTHTIKAGVSVALNRGTNFPGGTSFPVLQFGQFSFTGAFSGFD
                     YADFLLGLPQTAGRANAIPLQHWINRDFAFFVQDDWKITRKLTLNLGVRYEYNPPYHD
                     NEELMFNFDTATGRVVVPSERALQAVNPLFPRNLVPIVTAQEAGLPKSLYYTDLNNLV
                     PRFGFAYRPFANASTVIRGGYGIYIDDLTSQLWRLGAGGPYVSSESFVNRIANGSPEF
                     QFPRAFPAGFGAIGAQSFNALDPRFRNPYIQQWSLTVEQGIGNTGVRVSYIGTNSRGL
                     PWVRNINQPPPSTTPFNNNLRRYPELMNIQLRENGGLHNYNSLHIDVERKHRAGLYYQ
                     IG"
BASE COUNT          791 a         1234 c         1169 g          787 t
ORIGIN      
        1 gttaagccgc cgctcgacga cgagcaggtc gagaaaacgc tcgaccgcat tttgtcttca
       61 ccggcctttc gcaacagcaa gcagtgccag aaatttctgc gctacgtcgt gaatcacagc
      121 catgccggcg aggaagaccg gctcaaagag cgcagcatcg ggatcgaggt gttcggccgg
      181 gatcccggct acgatacggc cggcgatccc atcgtgcggg tccgcgcgac ggaggtacga
      241 aagcggctgg cccagtacta ccaggagtcc gctgtcgaag gcgacgtgcg ctttgagatt
      301 cccgccggtt cgtacaaggc cgagtttcac cctgtggcgg tgaggccggc cgtcatggaa
      361 ggcttacctc ccaggccacg ccgccggatc tggcggccat ggcttctggt gcctgcgctc
      421 gcagcattgc tcctggtggc cggctatttt gcgttacgcc cctctgaaac ggcggatatc
      481 ctggatcaat tctgggctcc ggtgattcgc aacccgaagc cggttttgtt gtattgtggc
      541 cagccggtgg tctattttct ttcgcgggaa gtgcgcgacc gatacgggaa agagccggcg
      601 gaccggcaga gcgcgtcaca gccggtggca ctgccgcccg acgccgtgat tcgtggccgc
      661 gacgtaattc cggtaacaga acagttcgtt ggcatcggca acgcccacac ggcggcgcag
      721 ctaacttccc tgttcgccgc gcggcacaag ccgattgaaa tccgctatgc caacgacctt
      781 tcgttcagcg acctgcgcgg ctcgccgtcc gtgctgatcg gcgccttctc gaatctttgg
      841 acgctcgaaa tgaccggtaa actgcgcttc gttttcgagc aggagaaagg agcgcgatgg
      901 atccgggacc ggtcgaagaa ccggacgtgg caactggacc ggctcgcttc cgacgggaaa
      961 acaccggaag actttgcgat cgtgtcgcgg ctgttcgact ccgccacggg ccagttgctg
     1021 atatcggcgg cggggatcac gcaatatgga acccgttcgg caggagagtt tctgacggac
     1081 tccagccgga tgcgggaggc gctggcgaag gccccgcccg attgggcgaa gcagaatatg
     1141 caggtcctgc tgcacaccac aatttacaga ggaaccccgg cgccgccaag agtactggac
     1201 gttcattttt ggtagtcgta tcattcgttc gacgcgcaca cccgctccgt gaccgtcgcg
     1261 gctcagaaag gccttactga gccgcgaccg caaggaagcg gacatcgaac tcctttttca
     1321 gcatcttgtt agatgaaggg ccgcccggaa atttcaagtg taaatggggc gacctgatat
     1381 atactggcgc tgtcagaggg ggattatgcg tcactgtatc ttggggatta ctgcgggcat
     1441 tctgctgatg gccgcaaatt tcgatctttt cgctcagact actttgggca acattctggg
     1501 ggctgccacc gatccaacgg gagcggctgt gcccggcgtg agtgtcacgg ttcgaaacct
     1561 ggggaccgga attgcgcacc aaaccgttac gcgcgaaagc gggctctacg aagtcacaca
     1621 cctgattccg ggccgctact ccgtttcggc cgagcggccg ggattcaaat cgatccaggt
     1681 gacgaacgtg tttctggaga cttcggctgc ggtccgtgtg gacctgccgc tggagatcgg
     1741 agaagtcact acgtcggtga ccgtcgaggc cgcggccgcc ccgatcaaca cggagagtgc
     1801 ggacgtagcc gccattcgcg gcaatgacgt gatggtcaag ctgccgatca acaatcggaa
     1861 cagcttttac ttcacgatgc tggtgctgac cccgggggcg atccgagggc agggctccaa
     1921 cgtgagcctg ggcggcgcgc gagggtttca gtggcactcc accgtggacg gaacgagcac
     1981 ccgctcgccc ctgttcgcca acgccgtagg accggcggaa agcggcatgg agatgacggc
     2041 cgaactgcgg attcagttgg caaacgataa ggcggaatcc gccctgccgg ggggctacta
     2101 cgcaacgtcg aagtctggaa cgaacgagta tcacggaagt gtgttctact atcactcgaa
     2161 ctcgcggctt accgcacgca acaccttttc gacctccgtt ccgttccaag tgcagaacaa
     2221 ctacggtgta gccgggggcg ggccgatagt caagaaccgg acgttcttct tcggcgttta
     2281 tgagcggttc ccgtcccgta gcgagcgaat tttctccaac acgaacgtgc cgacggcggc
     2341 gttccggaga ggggacttct cctcgctgct gcccggttcc gtgatacgcg atccgctcac
     2401 gaaccttccg tttccgaaca acgtcattcc cagcgaccgc atcagcagcg tgacgcagag
     2461 cgtgcaagag catttctttc cgctgcccaa cttcgggccc gagaacactt tccttcgcaa
     2521 ctggcggggc gtgggtccgg gaaagagttt caagtccatg gtggaagtgc gcgtggacca
     2581 tcgcctgtcg gcggccaact cgctgttcgg ccgcttctcc tggaatacta ccggcgccaa
     2641 cgtctacgac tacgacctgc ccactatgcc catccgcgat caggaccggc gcaccatcgc
     2701 cttcagcatc tccgatacgc acatcatcaa cccgtccact gtaaatgaat tccggttcgg
     2761 cgtcatgcgc agccgcaacc cggcgttcaa tccactagac ggacctgccc tggtcgaaaa
     2821 gttcgggctc gaaggcatag cgtggaaccc ggaaatcgcg acaggagcac cggtcttcag
     2881 cttcaataac ttctcgcaga tcggcgccac cgatctgtat caggacccca gcgagcgcat
     2941 ctaccagatc tcgaacaacc tgacctggac gcgctccaca catacgatta aagccggtgt
     3001 cagcgtggcg ttgaaccggg gaacgaactt ccccggtggc acgagtttcc cggtgcttca
     3061 gttcggacag ttctccttta ccggcgcgtt cagcggcttc gactacgccg actttctgct
     3121 cggtttgccg cagaccgcgg ggcgcgcgaa cgccattccg ttgcagcact ggatcaaccg
     3181 ggactttgcg ttcttcgtgc aggacgattg gaagatcact cgcaagctca ccctcaacct
     3241 tggtgtccgg tacgagtaca atccgcccta ccacgacaac gaggagttaa tgttcaactt
     3301 cgacaccgcc acggggcgcg ttgtcgtgcc gagcgaaaga gccttacagg ccgtcaatcc
     3361 gctgttcccg cgcaacctgg ttcccatcgt aacagcgcag gaggcgggtc tgccgaaaag
     3421 cctctactac accgatctga acaatctggt gccgcgtttc ggattcgctt accggccgtt
     3481 cgcgaacgcc agcaccgtga tccgcggcgg ctacggcatt tacatcgacg atctcacctc
     3541 acagttgtgg cggctgggcg cgggcgggcc ttatgtttcg tccgagtcct tcgtgaaccg
     3601 catcgccaac ggctcgcccg agtttcaatt tccgcgggcc tttcccgcgg gtttcggcgc
     3661 tatcggggcg caaagcttca acgcgctgga tccacgcttc cgcaatccct acatccagca
     3721 gtggagtctg actgtggaac agggaatcgg caataccggc gttcgtgtgt cctatatcgg
     3781 caccaactcc cgaggcctgc cctgggtgcg caacatcaac caacctcctc cgagcaccac
     3841 ccctttcaat aacaacctgc ggcgttaccc ggaattgatg aatatccaac tgcgcgaaaa
     3901 cggcggtttg cataactaca actcgcttca catcgacgtg gaacgtaagc atcgcgccgg
     3961 cctttactac cagatcggct g
//
LOCUS       DASTMK010000501         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_58641, whole genome shotgun sequence.
ACCESSION   DASTMK010000501 DASTMK010000000
VERSION     DASTMK010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58641"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFQ79_20730"
     CDS             complement(<1..714)
                     /locus_tag="VFQ79_20730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721518.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU0142160.1"
                     /translation="MSDPNLSTSSRTPVEEPTEGSTPRRRMVLNMGPQHPSTHGVLRI
                     VLELEGETILNATPDIGYLHTGIEKECEVKTYQQVVPLTDRVDYLANLSNNLGYSLAV
                     EKLLQLEIPPKAQWMRVLLVELTRLSSHLVWLGTHAMDIGAMTVFLYCFREREDLLRI
                     FEMFSGQRMMTSYIRVGGLALEPPRGWEQAVGKFIDAFPSKLAEYEDLLTKNPIWMNR
                     TKGVGFLALEDLLDCGVTGP"
     gene            complement(721..1206)
                     /locus_tag="VFQ79_20735"
     CDS             complement(721..1206)
                     /locus_tag="VFQ79_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU0142161.1"
                     /translation="MLPEILREIPVARALESWDPSAIVDGTAALGEAALYIAPEKILD
                     VCRQLRTEHGFARLGGITGVDWYPADPRFEVVYFLHSIGNNERLRLKCRVAEDQELDS
                     VTGVWRAANWYEREIYDLFGVTFRDHPNLRRIMLPEDWEGHPLRKDYPVHGHRYSYKD
                     E"
     gene            complement(1196..1612)
                     /locus_tag="VFQ79_20740"
     CDS             complement(1196..1612)
                     /locus_tag="VFQ79_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU0142162.1"
                     /translation="MPNTYIELYFPVVVQVVIAIAVAGGMIGGSFFLGKKVKNRIKDM
                     PYESGIVPTGTARERFSVKFYLVAMIFILFDIEAVFLYPWAVVYRELKLFAFFEMFVF
                     VALILSGFFYIWKKGALDWAVEEEPIDKPVREEHAA"
     gene            1685..1909
                     /gene="thiS"
                     /locus_tag="VFQ79_20745"
     CDS             1685..1909
                     /gene="thiS"
                     /locus_tag="VFQ79_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEU0142163.1"
                     /translation="MSGKGTNSIEIVLNGERKSVRPELTLTELLQELEIDPARVAVEL
                     DRFIVRAPEWPQTRLHDGAEVEIVQFVGGG"
     gene            2022..2531
                     /locus_tag="VFQ79_20750"
     CDS             2022..2531
                     /locus_tag="VFQ79_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0142164.1"
                     /translation="MFKTKKNRPEASTPARTFSSSEVARIGGVSLRQLQWWDERKVVS
                     PRHEGHRRVYFPEEVIEITVIAELRRKGFSLQKIRRVLRFLQREMGKRLADVLNHESD
                     LHLVTDGKSIYLEDRHDRIIDIIKNARQPMFLVCVSDQARRLSAATSPRKPARTESQA
                     AGRRAARAI"
     gene            2601..>3869
                     /locus_tag="VFQ79_20755"
     CDS             2601..>3869
                     /locus_tag="VFQ79_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU0142165.1"
                     /translation="MSDHRRLFLTFPLVILICALLGGAFGPSLDGVAVASAAGDDDIQ
                     KSLKIFTKVYDVVSENSAEQLDPDKAIYKGAIIGMLRTLDPHSSFFDPRDFRLLREDQ
                     RGHYFGVGMIVAPRSSKTIVIAPFPGSPAYKAGIRPGDVILEVNDRRTDNLTTTEVAD
                     LLKGPRGTAVQVVVARPGAEKPITFNIVRDEIPRKSVQDAFWLKPGVAYLDIDSFNEN
                     TGKEVEDNLKRLGEQNIEALILDLRDNPGGLLSEGVAVSDRFLEKGQTIVSHRGRSSP
                     EKRYVARQGARGKEYPIVVLVNRYSASAAEIVAGALQDHDRAWIIGENTFGKGLVQTV
                     YPLTGNTGLALTTAKYYTPSDRLIQRDYSNTSFLDYYYHNDLDQKNPLDVKMTDSGRT
                     VYGGGGIAPDEKFTTPKLSPFQTELLFRKYA"
BASE COUNT          855 a         1119 c         1110 g          785 t
ORIGIN      
        1 cggaccggtg acaccgcaat ccagcagatc ttcgagcgcg agaaatccta cgcccttggt
       61 ccggttcatc cagatcggat tttttgtcag caggtcttca tattcggcca atttcgacgg
      121 aaatgcgtca atgaacttgc cgacagcctg ctcccacccg cgcggcggct ccaacgcaag
      181 ccctcccacg cggatgtagc tggtcatcat gcgctgtccg gaaaacattt cgaaaatccg
      241 cagcaggtcc tcgcgttcgc ggaagcaata gagaaacacc gtcatggcgc cgatgtccat
      301 ggcgtgcgtt cccagccaga ccaggtggct gctgaggcgt gtaagctcca ccagcaggac
      361 gcgcatccac tgcgccttcg gcggaatctc cagttgcagc agtttttcga cggccagcga
      421 atagcccagg ttattcgaca ggttggcgag atagtcgacg cggtcggtta gcggcacgac
      481 ctgttgatac gtcttcacct cgcattcttt ttcaatgccc gtatgaagat aaccgatgtc
      541 gggtgtcgca ttgaggatgg tctcgccttc cagttcgagg acgatgcgca gcactccgtg
      601 cgtggacgga tgctgaggcc ccatgtttag gaccattcgc cgccgcggcg tactcccttc
      661 cgtaggttct tcgaccggcg tccggctgga agtggagaga ttcggatcgc tcatatcgaa
      721 tcactcatcc ttgtagctgt atcgatgccc gtgaacgggg taatccttgc gtaacgggtg
      781 tccctcccag tcctccggca gcatgatacg ccgcaggttt ggatggtccc ggaaggtgac
      841 cccgaacagg tcgtagatct ctctctcgta ccagtttgcg gcacgccaca caccggtaac
      901 agagtccagt tcctggtctt cggcaacacg gcacttcagg cgcaggcgct cattgttgcc
      961 gatggaatgg aggaaataga caacttcaaa ccgtgggtcg gcgggatacc agtctactcc
     1021 ggtaatgccg ccaagcctgg caaagccgtg ttcggttctc aactgccggc agacgtccag
     1081 gatcttttcg ggagcgatat acagcgccgc ctcccccagg gcggcggtgc catccacaat
     1141 ggcggacggg tcccacgact ccagtgcgcg agcgaccggg atctccctta ggatttcagg
     1201 cagcatgttc ttccctgacc ggcttgtcga taggctcctc ttccaccgcc cagtcgagcg
     1261 ctcccttttt ccagatatag aagaaccccg acaggatcag ggcgacgaag acgaacatct
     1321 cgaagaaggc gaagagcttc aattcacgat agacgacggc ccacgggtac aggaataccg
     1381 cctcgatatc gaagagaatg aagatcatcg cgacaaggta gaacttgacg ctgaagcgct
     1441 cccgggccgt tcccgtaggt acaatgccgc tttcataggg catgtctttt atgcggttct
     1501 ttaccttctt tccgaggaag aaagagccgc caatcatacc cccggcgaca gcaatcgcta
     1561 tcaccacctg aacaacgacg gggaaataga gctcgatgta agtattaggc atgaaagctg
     1621 gcgccggcca agaaccccta cacctcgaat ataatgagcg aagaaagggg gtgtcaaacc
     1681 gctgatgtct ggcaaaggaa caaattcgat tgaaattgtt ctgaatggcg agcggaaaag
     1741 cgttcggccg gagttgacgc tgacagaact gctccaggaa ctggagatcg atccggcgcg
     1801 ggtagccgtc gagctggacc gtttcatcgt gagagcgccg gagtggcccc agacccgctt
     1861 gcacgatggc gccgaagtgg agattgtaca gtttgtcggc ggaggataat atttggtctc
     1921 ttttctttca attaccggcc ccgtaaattt ccggtattga ctccccccgt caatccagta
     1981 caatcctatt ggcggttact cgaaaacgga agcccattct catgttcaaa acaaaaaaga
     2041 atcgccctga ggccagcacc ccggcccgca cgttctccag cagcgaggtt gcccgcattg
     2101 gcggggtcag cctgagacaa ttgcagtggt gggatgagcg gaaagtggta tccccacgcc
     2161 atgaagggca ccgccgggta tatttccccg aagaggttat cgagataacc gtcatcgccg
     2221 aactgcggcg taaaggcttt tcgctgcaaa agatccgccg cgtcctccgg tttcttcagc
     2281 gggaaatggg caagcggctt gcggatgtcc tgaaccacga gtcggatctg cacctggtga
     2341 cggacggcaa atccatctac ctcgaggacc ggcacgatcg aatcatcgat atcatcaaaa
     2401 acgcccgcca gccgatgttc ctggtttgcg taagcgacca ggcccgccgg ctcagcgcgg
     2461 ctacttcccc gagaaaaccg gctcgtacgg aaagtcaggc tgcgggacgc agggcggcgc
     2521 gggcaatctg acctctccga gtttgcgggg acccgtagtt gccaagatat acttgaatta
     2581 aaggtcctcg gaggtcccgg atgtctgacc atcgccgcct gtttctgact tttcctctcg
     2641 tcattctgat ttgtgcgctg ctgggtggcg cgttcggccc cagcctggat ggcgtcgcgg
     2701 tggcctccgc cgcgggcgac gacgatatcc agaaaagcct gaagatcttc acgaaagtct
     2761 acgacgtcgt cagcgagaat tcggccgagc agttggaccc tgacaaagca atctataagg
     2821 gcgccatcat cggcatgctg cggaccttgg acccgcattc gagcttcttc gatcctcgtg
     2881 atttccggct gctgcgcgaa gaccagcgcg gccattattt tggtgtgggc atgatcgtgg
     2941 cgcctcgcag cagcaagacg attgtgattg cgccgttccc cggctcccct gcctacaaag
     3001 cgggtatacg gcccggcgac gtgattttag aggtgaacga caggcgaacg gacaatttga
     3061 ccaccaccga ggtcgcggat ctgctaaagg ggccgagagg cacagccgta caggtggtgg
     3121 tggcgcggcc gggcgcggaa aaaccaatta cctttaacat tgttcgcgac gaaatcccgc
     3181 gcaagagcgt gcaggatgcc ttctggttga agcccggtgt cgcgtacctg gacatcgatt
     3241 ccttcaacga aaacacgggc aaagaagtcg aagataacct caaacggctg ggcgaacaga
     3301 acatcgaggc tttgatcctc gatttgcggg acaaccccgg cgggttgctg agcgaaggtg
     3361 tggccgtatc tgaccgtttt ctggagaagg gacagaccat cgtttcccac cgcggccgct
     3421 cgtcccccga aaaacggtac gtcgcccggc aaggcgcccg gggcaaggaa tatccgatcg
     3481 ttgtattagt gaaccggtac tcggcttcgg cggcggagat tgtggccggg gcgctgcagg
     3541 accacgaccg cgcctggatt atcggtgaaa atacgttcgg caaagggctc gtgcagaccg
     3601 tctatccact gaccggcaac accggcctgg cgctcaccac cgcgaaatat tacaccccga
     3661 gcgaccggtt gatacagcgc gattactcga atacctcttt tttggattat tactatcata
     3721 acgatctgga ccagaagaac ccgctggacg tgaagatgac cgacagcggc cggaccgtat
     3781 acggcggcgg cggaattgca ccggacgaga agttcaccac tcccaagctg tcgccatttc
     3841 aaaccgagct gttgttccgg aagtacgca
//
LOCUS       DASTMK010000502        17411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_441559, whole genome shotgun sequence.
ACCESSION   DASTMK010000502 DASTMK010000000
VERSION     DASTMK010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17411
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441559"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1138)
                     /gene="hemW"
                     /locus_tag="VFQ79_20760"
     CDS             complement(44..1138)
                     /gene="hemW"
                     /locus_tag="VFQ79_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU0142166.1"
                     /translation="MAGVYVSYPFCDQKCTYCNFASGVPSAGLESEYINAVAAEIAAY
                     RWQWSPETVYLGGGTPSRIPVEQLARLVDAIPGRPWREATIEVAPGTVTPGKAQAWKR
                     AGITRASLGVQSFVSRELARTGRKHTSETVARDVEQLRAAGIDRINIDLIAGLPFQNE
                     ASWRESLDWVGRLEVGHVSVYMLEVDEDSRLGLEILNGGGRYGARDIPDDDGIASFYQ
                     QAVEYLHKIGLRRYEISNFARPGSESLHNLKYWRLDPYIGFGADAHSFDGRTRRRNVE
                     SAGEYVDRFRRGLDPRAAETPAELSEERFFVGLRLEEGIRPASSELARFAQPIRRFLD
                     DGLLVMEDGFLRLTNRGVLLSNEVFQEFLT"
     gene            complement(1138..1467)
                     /gene="cyaY"
                     /locus_tag="VFQ79_20765"
     CDS             complement(1138..1467)
                     /gene="cyaY"
                     /locus_tag="VFQ79_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEU0142167.1"
                     /translation="MIDEQDFRRYADEAMSTLYRRLTAASDKHDFEADFNAGTLAIEF
                     EEPPAKFVISPNTPVRQVWVSAQSKSFKLDWNESRQAFVLPDKDQTLDELIGGAISDQ
                     LGEEVTL"
     gene            1552..3129
                     /gene="bshC"
                     /locus_tag="VFQ79_20770"
     CDS             1552..3129
                     /gene="bshC"
                     /locus_tag="VFQ79_20770"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEU0142168.1"
                     /translation="MEAKCIRQTGIPHTTRLFADYLYHFDRVSPYFLRNPHDPEVFRK
                     AASEVEFPASRRAALVEALRRQNGDSDALSRLSEHGTVAVVTGQQVGLFSGPAYTIYK
                     ALTAAKLAKQLSATGVPAVPVFWLATEDHDLEEVNHTWVFDSNHRAVALRAATKGTGQ
                     TPVGRMKLEDPPLGALREALKDFPFGGDIVSLVESTSPEGTTFGASFSGLLKALLGRF
                     GFLFLDPLDEGIRAIAAPLLVNALDAAPELSRQILARNSELTAAQYHAQVHFENETSL
                     FFRMDGAERKALKRRNGQYSGKDVRLSAEQLRDAPEQLSPNALLRPVVQDYILPTVSY
                     IAGPAELAYLAQSDVLYRGLLGRAPVILPRSAFTLLDSRAGKLMARYDLEFTTLLHGE
                     EPLRDRIAQHLVPPSLKDAFQKTLDSTSAQLQSLREQVVEFDPTLAASLDKSRAKMLY
                     QLEKCGRKVAREALRRNERAGEEAQFLSSLVYPHKHLQERFYSILPFLAKHGLDLLDR
                     LYENVHLGCPDHIVLEV"
     gene            3145..3930
                     /locus_tag="VFQ79_20775"
     CDS             3145..3930
                     /locus_tag="VFQ79_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0142169.1"
                     /translation="MRTNRVKQALREGKVQLGTSYGQIRSQEVAKILAAAGFQWAFID
                     TEHGGFGIETVQDVCRVSNLVGLCPIVRVADLQYALIARALDCGAQGIIFPRVESAEL
                     LQTAVNWMKFPPEGVRGCGVGPINVDWEPVSLPELTAHFNANIMVVLQIETRRAVEMR
                     DELLSVPGIDAVMIGPVDLSISYGVPGDFQHPKMVEAMELIKESCEKHGVAPGTQTRT
                     VTLAKFWRDRGMRFLGCSGDTTMLFERAKDLVSQLSMADGAAR"
     gene            3961..4049
                     /locus_tag="VFQ79_20780"
     tRNA            3961..4049
                     /locus_tag="VFQ79_20780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3995..3997,aa:Leu,seq:caa)
     gene            4142..5542
                     /locus_tag="VFQ79_20785"
     CDS             4142..5542
                     /locus_tag="VFQ79_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142170.1"
                     /translation="MKTTLLALALFCAVPAAAAGLRAGAAAVDVTPQEWPIRVIGNFG
                     LTLAKSAHDPLHARAVVLEDSGVRIAIVLVDSCFVKREEMDRAKALAAKRTNIPVNRI
                     LMAATHTHSAPPSRAEKGNRIEERYVERLVTQAAEAIVQANGRLQPAKIGWAVRPVPE
                     ELFNRRWFMREGGILPNPFGETGDLARMNPPAGSDNLIHPAGGTDPGFSIVSIQTAEG
                     KPLALLGNYSLHYIGGVPGPEVSADYFGEFAKQVASRLAPGDPAFVAMLTNGTSGDVN
                     NVDFVHPRPRAQPYERITAVAGKLAGYAVEHSHSMEYHATAPVQMVERELRVRYRRPT
                     PEQLRFARAALEEKDESKLPRNAKTYAGWAIALNDGPEFADLKLQAVRIGQLGIAAIP
                     CEVFTEIGITLKELSPFRPTFTIELANGSYGYLPTPRHFKLGGYETWLGTNNLEPEAS
                     VKITRTLLDLFGQIAK"
     gene            complement(5555..6832)
                     /locus_tag="VFQ79_20790"
     CDS             complement(5555..6832)
                     /locus_tag="VFQ79_20790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0142171.1"
                     /translation="MISANADSDGRMRRKVLGMLLAATTINYIDRQTLSVLAPLLESE
                     IGLNNLEYSYVVNSFLVVYSVMYLLAGGLVDRLGTRRGLGFAILWWSAAEMLHGAAIG
                     FKTLCLARALLAIGEAAIIPSGVKAVSEWFAPRERGVAVGTFEIGLSLGPILAPPLVS
                     LIALHWGWRHAFFWTGVLGLLWAVPWFLLYRTPKEMHSFKPDAAEPPVVARWISLLTS
                     RRIWAIGIARFFGDPIWYFYLFWLPKYLSESKGLSLAAIGAFAWIPYVGSLFGGLAGG
                     AMSGRLIARGWDPVRARKQVLLGSSIAASAGVLSIYIDKLGWALVVISIASFALQCWG
                     VNLDTLSSDILPSEQVAGAVGMCGLLGAAGGILFTAVTGFVVQNYSYTPIWIASALAY
                     PVGLSILWRLLPHGPATRRDDDTGKAHTIRSIA"
     gene            complement(6829..7722)
                     /locus_tag="VFQ79_20795"
     CDS             complement(6829..7722)
                     /locus_tag="VFQ79_20795"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0142172.1"
                     /translation="MDSKQIGASPGFTFAYYQLDSTINNFLWSLDALAQLGYENFGLE
                     TLEPEHTRLYWENDGIHRLLQRAEIRGIRFTSFTIWHCVTALCSCDSATRSLGVKRFA
                     EGVEIAKRLGIELVTIGSDWPSEWVTQYRPEYQHSPAGSYFIPSTREYEQTWCGYVDA
                     IGECLEIACRNNQRLAVEPRANSLIATADGFLRMWDRFRAAELGCALDVMHCSFQRES
                     VPVAIQKLGSQLEVFQVSGTDGATLHHLPLNSADETLLASIAALRAIGFTGPVDVELY
                     GMPVETVNEAYRQAREVLLAL"
     gene            complement(7754..9925)
                     /locus_tag="VFQ79_20800"
     CDS             complement(7754..9925)
                     /locus_tag="VFQ79_20800"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEU0142173.1"
                     /translation="MMKPSRRQFLLAAGAIAAPDGGRAQEAGEEFDWLRTARIVLAEG
                     YVPPFYPRLQCSGKQLASIAADLGANVLRFPAISQYALYPTRHYPVHPELGSRDLFQE
                     TIEACHAQGIRVIPYIAVNVSVWPKNTQGEVRDWIGVDAQGNPTTSTLFSLDREGNYL
                     QACLLSPIRDRIKAYVEEIVSKYDVAGMYFDGPYPYMSSQKCYCKYCSQAYQKAFGKP
                     VPRAGSGLSPEDERQYYRWFSQEVVAGLMKEIRQIVRTRKNVPVILNLTPFLFPRAYS
                     EAQYPISDAFMFESRPGPITNKMYNLVMARSTGKYIWSYVSTPHLESTEHLRKKRLGS
                     WYSSGVDGREVEMQGFTVIASGAAPIYWGANNRYRSLGNVQSLRRPYDFLQENAGILK
                     QLENARFAGIYTSTSTVDWYRYKPNGEAGYHPSFYYGAYALLKDLHIQAEPFHESQVT
                     GETLRRHRLVYLPNVACLSRAHADLIREYVHSGGALLATYETSLFDEWGKSRSDFALA
                     DVFGASWRESPEWGTDLFLRFEGGRSPQLFPEGTEIPHDPDFTLIEAAEENSMMGRVV
                     QPASKSIYTAGLVVRRFGAGRVAYISGSPEAVYYESRLAGPRELVRRVVDHLCGDEAP
                     PFHLEAPTGVFANLSRSERSLLLHVLADVGNKWKGLVSREEYVPVPGIVARIRIPRGR
                     TVRSARYLRSKAECRGEVVDGSLTLRMPPVEIYEGIYLEFA"
     gene            complement(9944..13198)
                     /locus_tag="VFQ79_20805"
     CDS             complement(9944..13198)
                     /locus_tag="VFQ79_20805"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0142174.1"
                     /translation="MLEVCLPFLSARRLAHVLIFTLCLPAAFAQQPVAAISGTVTDPT
                     GAAVPDALITATNVHTMLVRETRTSEGGSYVIPNLLIGEYRVEAQHAGFRRAARGPIS
                     LQVDQRATIDFQLQIGEVTGTVDVISEAPLLQTASSSVGQVVGTRMILDLPLNGRNFY
                     QLASLAPGVAFVPSGAPVVRSNYINVVMNGSRGHATAFLMDGVDTSEHHAGGTYVRPT
                     VDAIEEFKAISNNFSAQYGRSPGLITTAIRSGTNQFHGTAWEYLRNSSLDARNFFAGS
                     VAPLRYNQFGATLGGPVVRNRAFFFGSYEGTRVRQGLNFNVPVPSALNRSGDFSDARP
                     IYDPATTRPNPTGSGFIRDQFPGNRIPATRLAPQAQALLPYYPLPNTAGGTIATAPSR
                     ATTGDQVIGRFDQQITDKDQVFVRYTINYDRIQDPNSFPSLDPIPQLAKGQNAAVRYS
                     RIFSPTLINTVTLGYNRSRLFLDNAPNVKGSDGTTGSGILGFEETAPLYPGLPYLTMA
                     GFGTLNDGLDQPKQNVIENKQVLNDLTHIRGSHTLKAGFEYRRLKAITRDVAYNRGSF
                     SFTGTYTQNPAAPGGTGDAFADFLLGYPASALRSYPIETNGTFGNYFGLYFEDDWKVS
                     RKLTLNLGLRYEYSPFFTGLRNVIAGFDPSTGKIILPEPIDLTAQPVAVRAYPFYQDV
                     ITTTQSLGLPDSITPPEKTNFAPRLGFAWQPFERTATVIRGGYGIFWNYAINDILGNN
                     FLCPPFIIYDSATNDSPVPNRTLGNFYLGAPIPSAFGTPSISAAPVEGRNAYTNEWNL
                     SVQQALSATLSFEAGYVGQKGTHLQRRIPVNRPQPGPGSVQARRPFPRFSDISIGGWD
                     TDSTYHALQLRAEKRFSRGLAFTVSYAYSKSIDNNSNYFSGPLDVNNLKLERAVSDFD
                     LPHRLVASYTYELPFRLHARAARIVLEGWQVSGITTFQSGPPFTPALSSDRLNIGTGG
                     QRPDRIGSGNLDNPTIERWFDVSAFQTPALYVFGNAGRNILRGDGLVNWDLSAAKRFA
                     LSESLSLQFRGEFFNAFNNVNFSSPNAAVETANAGRVFGAGAARVIQFALRLSY"
     gene            complement(13245..13955)
                     /locus_tag="VFQ79_20810"
     CDS             complement(13245..13955)
                     /locus_tag="VFQ79_20810"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU0142175.1"
                     /translation="MQDLLKEVRPVRLFEDVALQLYQLIAKGHLSGGDRLPSEKQLCE
                     TFKVSRASVREALRSLGALGIVEVKNGGGTYVRKGSYESIGAILSMVLFHDFDDIAQI
                     YEARRAIEGWTSYTAARRITASQIAELEELVRGQQDALDRGESGVEEDFQFHLGIGKA
                     AGNEVIIRLLHSMITLIFRVLDPSKRPVRDLRVAVEQHGRILEALRRRDSAAAMQEML
                     EHIAVGMGPDDLAPNSAG"
     gene            14109..14663
                     /locus_tag="VFQ79_20815"
     CDS             14109..14663
                     /locus_tag="VFQ79_20815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0142176.1"
                     /translation="MGSDRRTAEILTELFDSSYATVVRYARRWTRDLAAAEDLAQEAF
                     FCLYRALQERKEIVCPLAWLMGVVRQMAEHQTWKRSKAQVSFSTLDTLDALPAGRTEP
                     DEPDFEVTRYFSCLTTREVEVVLLRMESLTYREVAEQMNISSKSVCTFLARALKKLRL
                     RMIQPATTTPVSSDEKKNIRRMLQ"
     gene            14629..15999
                     /locus_tag="VFQ79_20820"
     CDS             14629..15999
                     /locus_tag="VFQ79_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142177.1"
                     /translation="MRKRTSGECYSDEALLALLDGELAPVQAQQIGLHLNACWPCRSR
                     AGMLARNISELSALLDTRTIPPLAMERARARLLARCSAFDSVPELALRAPLGTAFSRI
                     ALSVAASLALCVLGVAAFVHFRPTGRIDRGSSSVPHPVRARSTQPVLPHPTANRMPDR
                     HPPRIPEPKSAPKVDLSRPTAARLLAAEIEAWHALHRVQACSGEPVEVLRQPDGRILI
                     RGVVAGLARLQEIRTAIGAVPDADLIDLDLTTIAEAIAANAPPQSVYSERSEVRQHPS
                     LAERYLLEHFRSQGLDSKSAHAEVVRISQQAVRQANSLLSAAWALRHLAERFRSEELQ
                     PESERLLTVLIRDHTRSIAVELAAQQRLLAPILPQSLDSVPVFRDGTPTFSRDWPDWA
                     LHHFSRIQHLHATTLDAFAGGDQTGLEFATFAQSLASDLRELENEFREASTGLSNWAG
                     RARKTH"
     gene            16027..>17411
                     /locus_tag="VFQ79_20825"
     CDS             16027..>17411
                     /locus_tag="VFQ79_20825"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142178.1"
                     /translation="MRKRLQAVSLLVLFAPIVLAQTAQVTGIVQDSSEAVVVGADVDI
                     NNVDRGVRYQVKTNELGYYTVPLLRPGNYRITIQAAGFKPVTRSNIVLETGQIVRIDF
                     VLEVGAVAEAITVSESVPLLQSENSTHSQFIESKTVMDMPLSGRRVENLVTLAGNAMM
                     ATGGSRVVLAGGRGGFVNWIVDGANSAPVLTEGLEFSQSPPVAELKEFRVMLNGYAAE
                     YGQSESGVVTLVTKSGGNQFHGSAYEYLRNDKLDARTFFAAGKAPLRQNLFGFTGSGP
                     IIRNRTFFFVSNEWQRVRRGTTSVLTVPTAAQRNGDFSQLTDAQGRSIAIYDPATSRP
                     DPTRAGNTLRDPFPGNVIPSNRLDPVGAGLAALYPMPNRAAANLAGASNFSANPVSFA
                     NPQYLTIKGDHILREHDRIWVKYNRVHSPDGQHPMYPEPAADPSAFTRESTYSAVIGN
                     WAHNFTPTLIN"
BASE COUNT         3592 a         5162 c         5140 g         3517 t
ORIGIN      
        1 cgtgtgacgg tgtcgctgcg aagatccgcc agcgctggtt cgctcatgtc aggaattcct
       61 gaaacacttc gttcgataag aggactcccc ggttcgtcag acgcaggaac ccgtcttcca
      121 tgacgagtag gccgtcgtcg aggaacctcc ggataggctg tgcaaaccgc gcgagttcgg
      181 agctcgccgg gcgaatacct tcctccagac gaaggccgac gaaaaagcgc tcctcggaaa
      241 gctcagccgg cgtctcggcg gcgcggggat ccaggccacg gcggaatcta tcgacatatt
      301 cgccagcgct ctccacgttc cgccgccgcg ttctgccatc gaaggaatgt gcgtcggcgc
      361 caaagccaat atagggatcc aggcgccaat acttcaggtt gtggagagat tcggaaccgg
      421 ggcgggcgaa gttggaaatc tcgtagcgcc gcaggccgat cttatgcagg tattccaccg
      481 cctgttggta aaacgaggcg atgccatcgt catccggaat atcgcgcgcg ccgtaacggc
      541 cgcctccatt caggatttcc agtccgagcc tgctatcctc atccacttcg agcatgtaaa
      601 ccgatacatg cccgacttcc agccgcccta cccagtcgag cgactcgcgc cagctcgcct
      661 cgttctgaaa cgggaggccg gcgatcaggt cgatattgat gcgatcgata cccgctgcgc
      721 gcaattgttc aacatcgcga gcaaccgttt cactggtgtg cttccggccg gttcgtgcca
      781 gttcccggga cacaaaggat tgcacaccga ggctcgcgcg cgtgatgccg gcgcgtttcc
      841 acgcttgtgc tttcccgggc gtcactgttc cgggtgcaac ctcgatggtg gcttccctcc
      901 agggccgtcc gggtatggcg tcgacgaggc gggcgagttg ctcgacgggg atgcggctgg
      961 gggtgccgcc ccctaaataa acggtctcgg gagaccactg ccagcggtac gccgcgattt
     1021 ccgcggcgac agcattgatg tactcgcttt cgagaccggc ggatggaact ccggaggcga
     1081 agttgcagta cgtgcatttc tgatcgcaaa acggatagga gacatagact cccgccatca
     1141 gagtgttact tcttcgccta gctgatcgct gatggctcct ccgatgagtt catcgagcgt
     1201 ctggtcttta tcgggtagca caaaggcctg tctggattcg ttccaatcga gcttgaagct
     1261 cttcgattgc gcggaaaccc atacctggcg caccggggtg ttgggactga ttacgaactt
     1321 cgccggcggc tcttcgaact cgatcgccag cgtgccggca ttgaagtcag cctcaaaatc
     1381 gtgcttgtcg gaagctgctg taagccgccg gtacaaagtg gacatcgcct cgtcggcata
     1441 cctgcgaaag tcctgttcgt cgatcatgac ctctgggaac tctccctcaa acttaacaaa
     1501 ttccagcaac tttgggatga accgcgtcat ctgctaacct tggacagttg gatggaagcc
     1561 aagtgtatcc gtcaaaccgg gattcctcat acgacgcgcc tcttcgccga ctacctctat
     1621 cattttgatc gggtttcacc gtactttctg cgtaatccac acgatccaga agtctttcga
     1681 aaggccgcgt ccgaggtaga gtttccggcc agccggcgcg ccgccctggt ggaagcgctg
     1741 cggcgccaga atggcgactc agatgccttg agccggttaa gcgagcacgg gaccgtcgcg
     1801 gtggtgacgg ggcagcaggt gggcttattt tcgggtccgg cctatacgat ctacaaagcc
     1861 ctgaccgcgg cgaaactggc gaagcaactc tccgcgacag gcgttcccgc cgttccggta
     1921 ttctggcttg ctaccgaaga ccacgatttg gaagaagtga atcatacgtg ggtcttcgac
     1981 tcgaaccacc gggcggttgc gcttcgggcg gcgacgaaag gcaccggaca aacaccggtc
     2041 ggacggatga agctcgaaga cccgccgctc ggagcgcttc gagaggcgct gaaggacttc
     2101 ccgttcggcg gggacatcgt ttccctcgta gaaagtactt cgccggaggg caccacgttc
     2161 ggagcttcct tctccggatt gctgaaagcg ctgctggggc ggttcgggtt cctgttcctg
     2221 gacccgctcg atgaagggat ccgggcgatt gcggcgccgc tgcttgtgaa cgctttggac
     2281 gcggcaccgg aactcagccg ccaaatcctg gcacgcaatt cggaattgac tgccgctcaa
     2341 taccacgcac aggttcattt tgaaaacgaa acgtcgctgt tcttccggat ggatggcgcg
     2401 gagcgcaagg ctctgaagcg gcgaaacgga caatattccg gcaaggatgt ccggttatcg
     2461 gcagagcagt tgcgcgatgc tccggagcag ctttctccta acgccctgct gcggccggtg
     2521 gtgcaggact acattcttcc caccgtgtct tacatcgccg gacccgcgga gctggcctat
     2581 cttgcgcaat ccgacgttct gtatcgcggc ctgctggggc gggcgccggt gattctgccg
     2641 agaagcgcgt tcacattgct cgacagcaga gccggaaagc tgatggcgcg ctacgatctg
     2701 gaattcacca cgctcctgca tggtgaggag cctcttcggg acaggatcgc acagcacctg
     2761 gttccaccat cattgaagga cgcgttccag aagacattgg actccacttc ggcgcaattg
     2821 caatctctcc gtgaacaggt ggtcgagttc gatccgacac tggccgcttc gctggacaag
     2881 agccgcgcca agatgcttta ccagctcgaa aagtgcggac gcaaagtagc ccgggaggcg
     2941 ctgcggcgca atgagcgggc cggagaagag gcacaatttc tttccagcct cgtctatccg
     3001 cacaagcatt tgcaggagcg cttctattcc attctgcctt ttctggcgaa gcacggactt
     3061 gatttgttgg accggcttta tgagaatgtc cacttgggtt gcccggacca tattgtgctg
     3121 gaggtctgag gtttccccgc gcttatgcga accaatcgag tcaagcaggc cttgcgcgaa
     3181 ggcaaggttc aactcggcac ttcctatgga cagatccgct cccaggaagt agctaagatc
     3241 ctcgcggccg ccggtttcca gtgggctttt atcgacaccg aacatggcgg gttcggaatc
     3301 gaaaccgtgc aggacgtctg ccgggtatcg aaccttgtgg gcctttgccc catcgttcga
     3361 gtggctgacc ttcagtacgc gctcattgcc cgcgcgctcg attgcggcgc gcagggaatc
     3421 atcttcccac gcgtggaatc tgctgaactt ttgcagaccg cggtgaactg gatgaaattt
     3481 cctccggaag gagttcgcgg gtgcggcgtg ggacccatta acgtggattg ggaaccggta
     3541 tctctccccg aactcactgc gcacttcaac gcgaacatca tggtggtgct gcaaatcgag
     3601 acccgccgcg ccgtggagat gcgcgacgag ctgctatccg ttccgggcat cgacgccgtc
     3661 atgattgggc cggtggatct gtccatctcc tacggggttc cgggcgactt ccaacacccc
     3721 aaaatggtgg aagccatgga actcattaaa gagagctgcg agaaacacgg cgtggcgcct
     3781 gggacgcaga ctcgcacggt gactctggca aagttctggc gggatcgcgg catgcgtttc
     3841 ctcggatgca gcggcgatac gaccatgctg ttcgagcgtg cgaaagacct ggtttctcag
     3901 ctttccatgg cggacggcgc ggctcgctga taaaatcggt atagctttca tatcactccg
     3961 gctggagtgg cggaactggc agacgcacgg gactcaaaat cccgcgccct tcactgggca
     4021 tgagggttcg accccctcct ccagcaccaa gaccacggga ataccgcggc ggcattgaca
     4081 cccttttttg cataacagca gcattgtgcg atagattctt ggcaatcggg atccttgctc
     4141 aatgaagact acgcttcttg ctctcgcctt attttgcgcc gtgcccgctg ctgcagccgg
     4201 gcttcgtgcc ggcgccgccg ctgtggatgt cacaccgcag gaatggccca ttcgggtgat
     4261 cgggaatttc ggattgacgc tcgcgaaatc agcccacgat ccgctgcatg cgcgcgcggt
     4321 ggtgctggag gactcgggcg tccggatcgc catcgtgctg gtggatagct gtttcgtcaa
     4381 gcgcgaagaa atggatcgtg cgaaagccct ggcggcgaag cgcacgaaca ttcccgtgaa
     4441 tcggattctg atggccgcca cacacacgca ctcggcgccg ccctcccgcg cggaaaaggg
     4501 caacaggatc gaagagcgat acgtggagcg gctggtgacg caggccgcgg aggcgatcgt
     4561 ccaggccaat gggcggcttc agcccgcgaa gatcgggtgg gccgtgagac cggtgcccga
     4621 ggagttgttc aatcgccggt ggttcatgcg ggaaggcggg attctgccga accccttcgg
     4681 cgagacgggc gacctggcgc ggatgaatcc gccggcggga tccgataatc tgatccatcc
     4741 ggcaggtggc accgaccccg ggttctccat tgtttctatc cagaccgccg agggtaagcc
     4801 gctggcgttg ctcggtaatt attcgctgca ttatatcggc ggcgtgccgg gaccggaagt
     4861 ctcggccgac tactttggcg agttcgccaa acaagtggcc agccgtctgg cgcccggcga
     4921 cccggctttc gtggcgatgc tcaccaacgg caccagcggc gacgtgaaca acgtcgactt
     4981 tgtacacccg cgccctcgcg cgcaaccgta cgagcgcatt accgccgtcg cgggcaaact
     5041 ggccgggtat gcggtggagc actcgcacag catggagtat catgccactg caccggttca
     5101 gatggtggaa cgggagctgc gcgtgcgcta tcgccggccg acacctgaac aactccgctt
     5161 cgcgcgggcg gcgctcgagg aaaaagacga atccaagctt ccgcgcaatg ccaagaccta
     5221 cgccggatgg gccatcgcct tgaatgacgg ccccgagttc gccgacctga agttgcaagc
     5281 ggtgcggatc gggcaattgg gaatcgcggc catcccctgt gaagtgttta ccgagatcgg
     5341 catcactctc aaagagctga gccccttccg gccgacgttc accatcgagc tggcgaacgg
     5401 aagctacggg tatctgccga cgccgcgcca tttcaagctc ggcggctacg agacgtggct
     5461 cgggaccaac aatctggaac ccgaagcttc ggtgaagatc acacggacgc tcctcgacct
     5521 gttcggacag atcgcgaagt aggcaccaga gggctcaggc gatagaacgg atggtgtggg
     5581 cttttccggt gtcatcatcg cgtctggtcg ccggaccatg cggcaaaagg cgccataaga
     5641 tggagagccc gacgggataa gcaagggcgc tcgcgatcca gatcggggtg taggaatagt
     5701 tctgcacgac gaatccggta acggcggtga acaggatgcc gccggccgct cccaacaaac
     5761 cgcacattcc caccgcaccc gccacctgtt cggacggcag gatgtcgctc gacagcgtat
     5821 ccagattcac tccccagcac tgtagtgcaa acgaagcgat gctgattaca accagcgccc
     5881 atcccagttt gtctatgtag atactcagca caccggccga agcggcaatg ctgctgccca
     5941 gcagcacctg tttgcgtgcc cgcaccgggt cccaccctcg tgcgatcagg cgcccgctca
     6001 tggctcctcc agcgagtccg ccgaacagcg atccgacgta tgggatccag gcgaacgcgc
     6061 cgatagccgc caggctcagt cccttgctct cggaaagata tttcggcagc cagaacagat
     6121 aaaagtacca gatgggatcc ccaaagaatc gggcgatgcc gatggcccaa atcctccggg
     6181 acgtgaggag agagatccaa cgggctacta ctgggggttc cgctgcgtcc ggcttgaacg
     6241 agtgcatttc tttgggtgtg cggtagagga ggaaccacgg gaccgcccat aacagtccca
     6301 ggacgcctgt ccaaaaaaat gcatgacgcc atccccaatg gagagcgatc aagctgacga
     6361 gcggaggagc cagaatgggg cccaggctga gaccaatctc gaaggtgccc accgcgactc
     6421 cccgttcacg gggcgcaaac cattccgaga cagccttcac tccgctgggg atgatggcag
     6481 cctctccgat ggctaacaac gcgcgagcca gacacagggt tttgaaaccg attgcggcgc
     6541 catgcagcat ctctgccgcg gaccaccata ggatggcgaa tcccagcccg cggcgcgtac
     6601 ccaagcggtc caccagtccc ccggccagca gatacatgac cgagtagacc accagaaagg
     6661 aattgacgac gtaggaatat tccaggttgt tcagtccaat ctccgattcg agcagaggag
     6721 cgagaaccga gagagtttgc cggtcgatgt agttgatggt ggttgcggca agcagcattc
     6781 cgaggacttt gcgccgcatc cgcccatcgg aatccgcgtt ggcggagatc atagcgcgag
     6841 cagcacttcc ctcgcctgcc ggtacgcctc attcaccgtt tcgacgggca tgccatagag
     6901 ctccacgtcc acaggtcccg tgaatccgat cgcccgcaaa gcggctatcg acgccagaag
     6961 cgtctcatcg gcgctattca acggaaggtg atgaagcgtc gcgccgtccg ttcccgatac
     7021 ctgaaaaacc tcgagttgag acccgagctt ctgaatggcg acgggaacgc tttcccgctg
     7081 aaacgagcag tgcatcacgt cgagcgcgca gcccagttcg gccgcgcgaa agcgatccca
     7141 catccttaag aacccgtcag cggtggcaat cagcgagttc gcgcgcggct ccaccgcgag
     7201 gcgctggttg ttccggcacg ctatttccag acactcgcca atcgcatcca cgtatccaca
     7261 ccaggtctgc tcgtattccc gcgtggaggg aataaagtag gacccggctg gactatgctg
     7321 gtattccgga cggtattggg ttacccattc cgaaggccag tccgaaccga tcgtcaccag
     7381 ttcgattcca agtcgttttg cgatttcgac gccctctgca aaacgcttca ctcccaggct
     7441 ccgggttgcg ctgtcgcaac tgcatagcgc ggtcacgcag tgccagatcg tgaaactggt
     7501 gaagcgaatc ccacggattt ccgctctttg gagcagacgg tgaatgccgt cattctccca
     7561 atacaaccgt gtatgttccg gctccagggt ttccagcccg aagttctcat aacccagttg
     7621 cgccagagcg tccaggctcc acaagaagtt attgatcgtg gagtcgagct ggtaatacgc
     7681 gaatgtaaat cccggagaag ctccgatttg ttttgagtcc atgaatgcgt tccttttcga
     7741 agaatgaaat tgatcaggca aactcgaggt agataccttc gtagatctcg accggcggca
     7801 ttcgcagcgt cagcgatcca tccaccactt ctccgcggca ttctgctttg cttcgcagat
     7861 accgggcgga ccgcactgtg cggcctcgag gaatacgtat ccgggcgaca atgcccggta
     7921 ccggcacgta ctcttcgcgg ctgacgaggc ccttccactt gttgcctacg tccgcaagga
     7981 catgcagcag taagctccgt tcgctcctgg aaaggttggc gaacacgccg gtgggtgctt
     8041 ccaggtggaa aggcggagct tcatcgccgc atagatgatc taccacgcgg cgtaccaact
     8101 cgcgtggccc cgccagtcgg gactcataat aaactgcctc cggacttccc gaaatgtaag
     8161 caacccggcc cgctccaaag cggcggacaa ccaggcccgc tgtgtagatg ctcttgcttg
     8221 ccggctggac tacgcgcccc atcatcgaat tttcttccgc cgcttcgatc agcgtgaagt
     8281 ccggatcgtg aggaatttcc gtgccttcgg ggaatagttg gggcgagcgt ccgccctcga
     8341 agcgaagaaa cagatcggta ccccactccg gggactcccg ccagcttgcg ccgaacacgt
     8401 cggcaagcgc gaagtcgctc cgtgatttgc cccactcgtc aaagagtgat gtctcatacg
     8461 ttgccagcag cgcccctccc gagtgcacgt actcccgaat caaatctgca tgtgcgcggc
     8521 tcaggcacgc aacgtttggc agataaacca accggtggcg ccgcagtgtc tccccggtta
     8581 cctggctttc gtggaatggc tccgcctgaa tgtgcaagtc cttcaggaga gcataggcgc
     8641 cgtaataaaa ggacgggtgg tatcctgctt ccccattcgg tttgtatcga taccaatcca
     8701 ccgtgctggt ggatgtatag atcccggcaa accgcgcgtt ttcgagctgc ttcagaatcc
     8761 ctgcgttttc ctgaagaaag tcatacggcc gccggaggct ttgcacgttc cctaaagacc
     8821 ggtaccggtt gttagcgccc cagtagatgg gggctgctcc ggatgcaatc acggtgaagc
     8881 cctgcatttc gacttcccgg ccatcgacac ccgacgaata ccaggagccg agccgcttct
     8941 ttctcaggtg ttccgtgctc tccagatgcg gcgtgctgac atagctccag atgtatttgc
     9001 cggtcgaacg ggccatcacc aggttgtaca tcttgtttgt gatcgggccc ggccggcttt
     9061 cgaacatgaa ggcatcggat atcggatact gcgcctccga atacgcgcgt ggaaacagga
     9121 acggtgtgag attcaggatg accggcacat tcttccgagt gcgaacgatc tgccggatct
     9181 ctttcatgag ccccgctact acttcctggc tgaaccatcg gtaatactgc cgctcgtctt
     9241 cgggagagag gccggagcct gcgcgtggaa ccggcttgcc aaatgctttc tggtaggcct
     9301 ggctacagta tttgcaatag cacttctggc tcgacatgta gggataaggc ccgtcgaaat
     9361 acatgccggc cacatcgtat ttggagacga tctcttctac gtatgctttg atcctgtccc
     9421 ggatcggtga aagtagacag gcttgcaggt aatttccctc gcggtcgaga ctgaaaagcg
     9481 ttgaggtcgt cggattgcct tgggcgtcga ctccaatcca gtcgcgaacc tccccctgcg
     9541 tattcttagg ccagacactg acgttgaccg ctatgtaagg tatgacacga ataccttgtg
     9601 cgtggcacgc ctcgattgtt tcctgaaaga ggtctcgcga accgagttcg gggtgaaccg
     9661 ggtagtgccg ggtcggataa agcgcatact gcgaaatcgc ggggaagcgc agcacattgg
     9721 ctcccaggtc cgccgcaatg gacgcaagct gttttccgga gcattgcaat cgcggataga
     9781 agggcggaac atagccttcc gcgagtacga ttcgcgctgt tcgcagccag tcaaactctt
     9841 caccggcctc ttgggcgcgc ccgccatcgg gcgccgcgat ggcacccgct gccagcagaa
     9901 actgccttct ggatggtttc atcataagag ttgtgattcc ggctcagtag ctcagccgca
     9961 gggcgaactg gataacccgg gccgcacccg cgccgaatac acgtccggcg ttggcggtct
    10021 cgacggccgc attcgggctc gaaaagttca cgttgttaaa tgcgttgaaa aattccccgc
    10081 ggaactgcag ggacaagctc tcactgaggg caaagcgctt ggcggcggag agatcccagt
    10141 tcacgagccc atcgccgcgt aggatattgc gtcccgcatt gccgaagaca tagagcgcgg
    10201 gcgtctggaa agcggacacg tcgaaccagc gttcgatcgt cggattgtcg agattgcctg
    10261 agccgatgcg atccggacgc tggcctcccg tgccaatgtt cagacggtcg ctggagagcg
    10321 ccggagtaaa gggcgggccg ctctggaacg tggtgatgcc gctgacctgc cagccttcca
    10381 ggacgattct ggccgcgcgt gcgtgcaggc ggaacggaag ctcatacgtg taactcgcta
    10441 ccaggcggtg cggaaggtcg aaatccgaaa cggcccgttc cagcttgaga ttgtttacat
    10501 ccagaggccc ggagaagtag ttcgaattgt tgtcgatgct tttcgaataa gcgtaagaaa
    10561 ctgtgaacgc caatccgcgt gagaacctct tctcggcccg gagttgcaga gcgtgatatg
    10621 tcgaatcggt atcccagccg ccgatggaaa tatcggagaa gcgtggaaac ggccgccggg
    10681 cttgcaccga tcccggaccc ggctgcgggc ggttaaccgg aatccttcgc tgcaagtgag
    10741 tgcctttctg tcccacatat ccggcttcga aggaaagcgt tgcggataac gcctgctgaa
    10801 cgctcagatt ccattcgttg gtgtaggcgt tgcgtccttc caccggagcg gcggagatgg
    10861 agggcgttcc gaacgcggaa ggaatcggcg ctccaagata gaagttgccg agtgtccggt
    10921 tcggcaccgg ggaatcattc gtagccgagt catagatgat aaacggcggg cacaggaagt
    10981 tgtttcccag aatatcgttg atggcatagt tccagaaaat gccgtatcct ccgcggatga
    11041 cagtggcagt tctttcaaac ggttgccacg cgaatccgag ccgcggtgca aaattggtct
    11101 tttcgggggg tgtgatcgaa tccggcaatc ccagagactg cgtggtggta atcacgtcct
    11161 gataaaacgg ataggcgcgc acggccaccg gctgggccgt cagatcgatg ggctcgggca
    11221 ggatgatctt tccggttgaa ggatcgaagc ccgcgatcac gtttcgcaaa ccggtgaaga
    11281 agggactata ttcataacgc agcccgagat tcaaagtgag tttccggctg accttccaat
    11341 cgtcctcaaa gtacaagccg aagtaattgc cgaatgttcc gttggtttcg atcgggtaac
    11401 ttcgaagcgc cgaggcggga taccccagaa gaaaatccgc gaacgcgtcg cccgttccgc
    11461 cgggagcagc ggggttctgt gtataggttc cggtaaacga gaaagagccc cggttgtagg
    11521 caacgtcgcg cgtgatggcc tttagccgcc tgtactcgaa gccggctttc agcgtgtgac
    11581 tgccacgaat gtgagtcagg tcgttgagta cctgcttgtt ctcaatcacg ttctgcttgg
    11641 gttgatcgag cccgtcgttc agcgtgccga aacccgccat cgtgagatag gggagccccg
    11701 gatagagcgg cgcggtctcc tcgaaaccca gaatgccgga gcccgtcgta ccatccgaac
    11761 cctttacgtt gggagcgttg tccagaaaca ggcgcgatcg gttgtagccc aacgtcaccg
    11821 tgttgatgag cgtggggctg aagatgcggc tgtaccggac ggcagcgttc tggcctttgg
    11881 caagctgcgg gatggggtcc agcgacggaa acgagttcgg atcctggatg cggtcgtagt
    11941 tgatggtgta gcggacgaac acctgatctt tgtccgtgat ctgctgatcg aagcggccga
    12001 tcacctggtc gcccgtcgta gctcgggagg gtgcggtcgc gatggtgcct ccagccgtat
    12061 ttggcagcgg gtagtaaggc agcaaagcct gtgcttgtgg cgccagtcgg gtcgcgggaa
    12121 tgcggttgcc cgggaactgg tcacggatga agccgctgcc tgtgggattg gggcgcgtcg
    12181 tggcggggtc gtagatcgga cgcgcatcgc tgaaatcccc cgagcgattc agagcgctcg
    12241 gaaccggcac gttgaagttc aagccctgcc ggactctcgt gccttcgtac gatccgaaaa
    12301 agaaagcccg gttgcgcacc accggaccgc ccagcgtggc gccgaactgg ttgtagcgca
    12361 gaggagccac gctcccggca aagaaatttc tcgcgtccag actgctgttg cgcagatact
    12421 cccaggcggt tccgtgaaat tgattcgtcc cggaccggat cgccgttgtg atcagcccgg
    12481 gggagcggcc gtactgtgcc gagaagttat tcgaaatggc tttaaactcc tcgatcgcat
    12541 ccacggtagg gcgcacgtag gtcccgcccg cgtggtgctc gctcgtgtcg acgccatcca
    12601 tcaggaacgc cgtagcgtgc ccgcgcgatc cgttcatcac cacgttgatg tagttggagc
    12661 gaaccaccgg cgcacccgaa ggcacgaaag ccactcccgg agccaggctc gcgagttgat
    12721 agaagtttct gccattcaga ggcagatcca ggatcatcct ggtcccgacc acctgcccca
    12781 cggagctgct ggctgtttgc agcagaggcg cttcgctgat gacatccacc gtgccggtta
    12841 cctcaccgat ttgcagttgg aagtcgatgg tggcccgctg gtccacctgg agggaaatcg
    12901 gtccgcgagc cgcacggcgg aaaccggcgt gctgcgcttc tacccggtat tctccaatga
    12961 gcagattcgg gatgacgtag ctgccccctt cactcgtacg ggtttcgcgg acgagcatcg
    13021 tatggacatt ggtcgcggtg atcaacgcgt ccgggacggc cgccccagtt ggatccgtaa
    13081 cagtaccgga gattgccgcc actggctgtt gcgcgaaggc ggcaggcagg cacaacgtaa
    13141 agatcagcac gtgcgccagg cgcctggcac tgagaaacgg tagacacact tcaagcatga
    13201 gcgcctccta tcggcgtcag ttagccccaa ttaagctgtg atggttaccc cgcggagttt
    13261 ggggcgaggt cgtccggccc cattccaacc gcaatatgtt ccagcatctc ctgcatcgcc
    13321 gcagcagagt cgcggcggcg cagcgcctcc aatattctgc cgtgctgctc cacggccacc
    13381 cgcaaatccc ggaccgggcg cttggaagga tccagcaccc gaaaaatcag cgtgatcatg
    13441 gagtgcagga gacgaatgat tacttcattc cctgctgcct tgccgattcc caggtgaaac
    13501 tggaagtctt cttccacccc actttcgccc cggtctaatg cgtcctgctg tccccgaacg
    13561 agttcttcca attccgcgat ctgactggcg gtgatccgcc gtgccgccgt ataggacgtc
    13621 cagccttcga tcgcacgccg cgcttcgtaa atctgcgcga tgtcatcgaa gtcgtgaaac
    13681 agaaccatcg ataagatggc cccgatactc tcgtaggatc cttttcggac gtacgtcccg
    13741 ccgccgtttt taacctccac gatccccaaa gcgccgagac ttcgcagcgc ctccctcacg
    13801 gaagcacggc tgaccttgaa cgtctcacaa agctgttttt cggaaggaag gcggtcgcct
    13861 ccgctcaagt gaccctttgc aatcaattga taaagctgga gagccacatc ctcgaacaat
    13921 cgcaccggcc gaacttcttt aagaagatcc tgcattatgt ctagctctct acctgtctga
    13981 caggtagaca ttagcgtatt tcagaaggga tgtcaatcct taattcgcgg atttctttag
    14041 aagacgtcag gtaaatccag ctaaagtgac actgaacagt aggatagaac gctggaggtc
    14101 ggcgccgagt gggatccgat cggagaaccg cagaaatact gaccgagtta ttcgatagca
    14161 gctacgcgac ggtcgtacgc tacgcgcggc gctggaccag ggatcttgcg gccgccgaag
    14221 atctggccca ggaggcattc ttctgtctgt acagggcttt gcaggagcgt aaggagatcg
    14281 tctgcccact cgcctggttg atgggcgtag tcagacagat ggccgaacat cagacgtgga
    14341 aaagatccaa ggcccaggtg tctttctcga cgctggacac cctggatgcg ctaccggcag
    14401 gccgaaccga gccggatgag cccgattttg aagtcacgcg ctatttctcc tgtctcacca
    14461 cacgggaggt agaagtcgtg ctgcttcgga tggaatctct cacctaccgg gaggtagccg
    14521 aacagatgaa catcagttcc aaatccgtct gtacgttcct ggcccgtgcg ctgaagaaac
    14581 ttcggctccg catgatccaa ccagcaacta ccactccggt ttcaagcgat gagaaaaaga
    14641 acatccggcg aatgctacag tgacgaggcg cttctggcgc tcctcgatgg cgaacttgcg
    14701 ccggtgcagg cgcaacagat cgggctgcat ctgaacgcat gttggccctg ccggtcccgt
    14761 gcgggaatgc tggcacgcaa tatatcggag ctttccgctt tgctcgacac caggactatc
    14821 ccaccgctgg cgatggaacg ggcgcgggca cgtctcctcg cacgctgttc cgcgttcgac
    14881 tccgttccgg aactggcact ccgcgcaccg ctgggtacgg cgttttcgcg aattgctctc
    14941 tctgtggcgg cgagcctcgc tctctgtgtt cttggagtgg ccgcgttcgt tcatttccgc
    15001 ccaacgggac ggattgacag aggctcttcc agcgtgcccc atcccgtgcg cgcgcgatcg
    15061 acccaaccgg tgctcccaca tccaaccgcc aatcgaatgc ctgatcggca tccaccacga
    15121 attcctgaac cgaagtccgc acccaaggtg gatttgagcc gtcctacagc ggcgcgatta
    15181 ttggcggcgg agatcgaagc ctggcacgca ttgcaccggg tgcaagcctg ttcaggtgag
    15241 cccgtcgaag tacttcggca gccggatggc agaattctta tacgcggcgt agttgccggc
    15301 ctcgctcgtt tgcaggaaat acgaacagca atcggcgcag ttccagatgc agatctgatc
    15361 gacctcgacc tgacgacgat agccgaggcg atagccgcga acgcaccacc gcaatccgta
    15421 tacagcgaaa ggtcggaggt gcgtcagcac ccttccctgg ccgaacgtta ccttctggaa
    15481 catttccgct cccaaggctt ggacagcaag tctgcacacg cggaggtcgt ccggatttcg
    15541 caacaagctg tccggcaagc gaattcactc ctctctgctg cctgggcttt gcgtcacttg
    15601 gccgagcgtt tccgttccga ggaattacag ccggaatccg aacggctcct taccgtcctc
    15661 atcagagacc atacgcgttc gatcgccgtg gagttggccg cgcaacagag attgctcgct
    15721 ccgatccttc cgcagtcatt ggattctgtc cccgtgttta gggacggcac tcccacgttt
    15781 tcacgggact ggcctgactg ggctctccac catttctcgc ggatccagca tctgcatgcc
    15841 accaccctcg acgcttttgc gggcggggac cagactggtc tggagtttgc cacatttgcg
    15901 caaagccttg cttcagattt gcgcgaactt gaaaacgagt ttcgggaagc cagcacaggt
    15961 ttgtcgaatt gggccggcag agcccgcaaa acgcactgaa ccgtcaaccg gcaagaaagg
    16021 atagctatgc gaaagagact ccaggcagtt tcattactcg tgctgtttgc gccaatagtc
    16081 ttagctcaga ccgcccaggt gacgggtatc gtacaggact cgagcgaggc ggtagtggtt
    16141 ggggctgacg tcgacattaa caacgtagac aggggagtcc gataccaggt caagacaaat
    16201 gaactcggct attacaccgt tccactcctg aggcccggca attaccggat taccattcag
    16261 gcggccggat tcaagccagt aactcgaagt aacattgtgc tcgaaaccgg tcagatcgtc
    16321 cgcatcgact ttgtgctgga ggtaggcgca gtcgcggaag ccatcacagt gtcggaaagt
    16381 gtaccattgc tccaatcgga aaactcgaca cattcccagt ttattgagag taagacggtg
    16441 atggacatgc ctctcagcgg gcgccgtgtc gaaaacctgg tgacactggc agggaacgcc
    16501 atgatggcga cgggcggatc cagagttgtg cttgcgggcg gacggggcgg gtttgtgaat
    16561 tggattgtcg acggtgccaa ctccgcccct gtactgactg aaggtttgga attcagccaa
    16621 agtccacccg tggctgagct aaaagagttc cgggtgatgt tgaacgggta cgccgccgaa
    16681 tacggacaaa gtgaaagcgg tgtcgtcacg ttggtgacaa agtccggcgg gaatcagttt
    16741 cacggaagcg cctacgagta tctgcgcaac gataaactgg acgcccgtac ctttttcgcg
    16801 gcggggaaag cgccactgcg ccagaacctg ttcgggttca ccggcagcgg tcccatcatc
    16861 aggaatcgaa cctttttctt tgtttcgaat gaatggcagc gtgtgcggcg aggcacaaca
    16921 agtgtattaa cggtacccac cgctgcgcag cgcaacgggg atttttcgca gctcacggac
    16981 gcacagggcc gctccatcgc gatctacgat ccggcaacca gccgtcccga tccaacccgg
    17041 gcggggaaca cccttcgcga tccgttcccc gggaacgtga ttccttcgaa ccggcttgat
    17101 ccggtgggtg ccggtctggc tgctctgtac ccgatgccaa atcgcgcggc cgccaatctc
    17161 gccggtgcga gcaatttttc cgcgaaccct gtgagctttg ccaatcccca gtacctgacc
    17221 atcaaagggg atcacatact ccgggaacac gaccggattt gggtgaagta caaccgcgtc
    17281 cattcacccg acgggcaaca tccgatgtat cccgagcccg cggccgaccc ctccgcgttc
    17341 actcgggaat ccacatacag cgccgtaatt ggaaattggg ctcacaactt cacgccgact
    17401 ctgatcaatg a
//
LOCUS       DASTMK010000503        12771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_568473, whole genome shotgun sequence.
ACCESSION   DASTMK010000503 DASTMK010000000
VERSION     DASTMK010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568473"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFQ79_20830"
     CDS             <1..559
                     /locus_tag="VFQ79_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142179.1"
                     /translation="PRFDYASMGDAIAVQRLTSFFGSAGISLKALPAHLAAWESIKDN
                     NLIFIGAWRMHPLLRRLPVTQDFELGPDDQIHNQKPGPGELKIYTTPSHRDSMTYASV
                     GVFPGLRPGREVFVVTAHSSPGAMGAVDFITNAGSVKLIRDRIGLSSSGPRKYFQMLL
                     RVYVDNDVPMKTEYVTHHLNPQPLR"
     gene            639..2519
                     /locus_tag="VFQ79_20835"
     CDS             639..2519
                     /locus_tag="VFQ79_20835"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU0142180.1"
                     /translation="MRIIFSLLVWAGPAGVALAQFPEMPPSTLAAPKPAFDDGQDAMR
                     TRLFRTTTSRAQIDLGGWWDFLPDPGDKGEKLNYGSRLPRPETRLWVPGTWNSVARYW
                     NYVGPAWYQRDFEIAGGGNLRIMFGSVFYRSKIWLDGKLLGEHEGGYLPFAFTVRDVK
                     KGPHTLVVRVDNRLDDASLPKQNVDWFPYGGIERPVYAEMVPSVFIDSFHVIPSSIQP
                     SRASLKVRVFLWNLGQSSGTVELAFSVNGTRLHAVRHAAASGRSAAEFEVMLERPRLW
                     SPADPNLYSARISLNGDGDDQFTRFGVREVRVDGPRILLNGRPLKVMGVNRHEDHPDW
                     GSGSPPNLLRQDIEIIKRLGANAVRAHYPLPELFMDFCDQYGLLFMAEAPAWQYSSEQ
                     LAKPEIQEKVLRHFEEMVARDMNHPSVFTWSLGNEWPQPDRSYDVIRDLLARARRFDS
                     TRLITLVTGGPHVRRIHELVDVICVNWARYQWYDPFTTLDAEEGAKSVAELAKIHERY
                     PGKPVILTEFGGSEAQAGWHNWGNVKWSEEYQARNVEDSAWHALQQDWISGGCVWQFC
                     DTRSAPERFLAGRLHGWNGKGIVDANRNPKLAFYKLQQIFRAIAEPAATSEGTPSSDA
                     GK"
     gene            2576..3712
                     /locus_tag="VFQ79_20840"
     CDS             2576..3712
                     /locus_tag="VFQ79_20840"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEU0142181.1"
                     /translation="MKTSNRLFAIILLFACCGWAGERKAKNVILFLGDAAGTSTLHAA
                     SVQEYGDSNRLFLYRMPNIALSDTSSAADWVTDSAAGMTAIVTGEKTNNGVVSQSDSA
                     VRGKKDGEPLKTLLEYAEERGLSTGVVSNSPITDATPAACYAHVNDRKMTGEIFRQAL
                     NPRFGDGVDVMIGPGRQAILTATRELGMNLETEIPAKGFHFGETLDQVPPDARRAIVL
                     LGPGEFDLGAATELAIRILSKNPKGFFLMVESDLHTDNLSRGLTRAVAFDRIIESTAA
                     SASKDTLILFTADHSFDIRVGGATRKGLPLVTQDADGKPVPAKSVMINGHHSAEQVLV
                     AAQGPGASRVRGVIANTGIFHIILSAYRWKPEPAHTPPQITSGN"
     gene            3782..6175
                     /locus_tag="VFQ79_20845"
     CDS             3782..6175
                     /locus_tag="VFQ79_20845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142182.1"
                     /translation="MLQDIRFGLRMLACNPGVTVLATIALALGIGGSTVVFSVVNTVL
                     LRPLPVSDPDRLVWIWANNPSRNLAYAFTAYSSYAEWKAGSPSLESMSAYAPVSATLL
                     LGNAPERVDVLKVNATYFPMLGIQPVVGRNFLAEEDQPGARRVAILSYDLWERRFGRD
                     PDVIGRTINLEGESVAVTGVLPRGFAFPNKVADVYVPIAHSTARATGGAPPLFVGAYG
                     RLKPGVAIERAQAEIDVVSRRLEAAYPGMKGMGAQVWRVRDFMVRDVRLSLLVLFGAV
                     GLVLLIACANVANLLLVRASVRQREIALRTALGAGRWRILRQLLIESVILSVCGGVAG
                     LALAAAGTRLVRRSGPEQIPFLKEVSLDSRVLAFAILASLLTGVLFGLAPALAAFRAR
                     VSETLKEGAIAAGESRARNRFRGALVVIEVAIALVLAVGATLTMRTLLRLQATSPGFD
                     AGGVLTASITLPTPKYPKPEQRVDFYQRLLDRTNVMPGVQAASMVSLIPFGGSDTGWN
                     VLIEGEPAPRRWDAPVSWRRIVDPAYFRVMRIPLIRGREFTSHDSGSPLVAIINETMA
                     RRFWPNTDPLGKRFGLTKYWLTVVGIVGDVKFTSFTKDAEPEFYEPYRQAPGADMTLT
                     VRTVSDPLRLAPALRQAVLETDPTQAISRVTPMAQYVSDAVGTPRVSALLLAAFGTTA
                     LLLAAVGIYGVISFSVTRRTREIGVRIALGAAGRDVMRIVVGDAIVLASIGVAVGIGG
                     ALALNSIMQRMLFGVTATEPFTYLAVSALLIAIAALAAYVPARRAARISPSVALRCE"
     gene            complement(6317..6574)
                     /locus_tag="VFQ79_20850"
     CDS             complement(6317..6574)
                     /locus_tag="VFQ79_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0142183.1"
                     /translation="MATTAQRRQRIDSHRSRQFLAELAALPAERYSHTPLIGRMWELR
                     HNFTVYDAAYIALAEATGSVLYTCDEKLRSEHRARVVLFTS"
     gene            6909..8129
                     /locus_tag="VFQ79_20855"
     CDS             6909..8129
                     /locus_tag="VFQ79_20855"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0142184.1"
                     /translation="MNRRQFFATAAATGSASSAGTSSGSPDFSAVRADFPRARTAAYL
                     DNASCHPLSMHAAAALHRYIDWATKEVGEPWWPGWAQARTEAKTLFARLINAKPSEIA
                     FARSTIEAESNLLNGMAIRASGGNVVTNDLHYSAAIYNYRMRQKDGLDVRIVKHRDWQ
                     IDIRDMERAVDRNTRLIAIALVSNVNGYLHDVRKLSELAHSHGAYLYADIIQCAGAIP
                     IDVRAMGIDFAACSTYKWLMGVKGFGFLYVREDLQGTVVKPAQHSGGVRFNYAPWVEQ
                     PDAGLDEIAFSPVAGPSRYEVSYPSYEGAICALESLKYIHRLGVGNIRAHARALTGRL
                     QKEMPAAGYPSITPPDNESPIVAFATKDPQRAMEKLRKAGVHAAMRFGNKMRISPSVF
                     NNQEDVDRLLAALA"
     gene            complement(8138..10402)
                     /locus_tag="VFQ79_20860"
     CDS             complement(8138..10402)
                     /locus_tag="VFQ79_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142185.1"
                     /translation="MTEVFLRTACAAALAFSVASAQDGPSAAEFAEKALPALPVEKSY
                     TFQKVLGERVEPLRRDPAARPAATEIAIPPQGWSLVIRSDSGPLLRHAAVEFRERIAD
                     GMQVNLKLETPGTLDGWAAMSRSVVAGTRDQMPGCGQALKGPKDYQIQVKGERIAVCG
                     FDERGAMYGLYNVEARFSLREAPFLSRTLDTTRHSLYRTRMTLSGLGWMNWPDRFLAT
                     LVRYGFDAIHDSIYRNPNNAPGVGPHWDKMKEHPPGAMADLVRRAAKYGIGVYVPILW
                     RYTPTPENEEGLRKLVRDIVTEFPDIRGYVLLTEGFYYERWFGAGGQGDQDLRKWLRE
                     WARAVAIVAEECRKINPAIEVLPWEYNVDFRPHQAENKRYGTAQLPRDSIPLLTFENG
                     KAFELDGERGWLKDYAINQVGPAEVTQAQIAEAKKLGFRGVYTKADTFASWQYGTFPY
                     LPFPYQWYERYKALEKWGVDGVLESWSYGFKPNFVAELRAWYSWSDAPPLDELLRAIA
                     RRDFGAGNEDAVLSAWKCFSNAIRLVPDTGPNWGTNNAVAAPFFLTQPEPRMMTVNHS
                     WSDPALWARTTWVNPYWPYVPSRLILWPDFTNKVNAAERYVRPFSLPVFSKHLGLAAA
                     EMEKGLQHYRKAALAAPAAKRQNAYRETLLAEQLQRMMRSEQALLEFEDLRFRLGQNA
                     AGKTAVLDRLEAILREEIERTEASFEAARRDSRLGYEWEQDYIYTPATIHEKLLLLKR
                     TLAEDLPALRNGAQ"
     gene            complement(10439..11569)
                     /locus_tag="VFQ79_20865"
     CDS             complement(10439..11569)
                     /locus_tag="VFQ79_20865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0142186.1"
                     /translation="MALSPVVETLAGLVRINSINPAYESGTSEAGLVEYIEQFFAKRS
                     IRTWRQEVFPGRPNLIAAVPGRQSSRRLILEAHTDTASVKGMTIPPFEPRVEGGRLYG
                     RGACDTKGGLAAMMHALAAVAEGGRKPPCEVWLVAAADEEYSYRGVVKLCEGLTAHGA
                     VVAEPTSMRLVTATKGCVRFKVHTRGKAAHSSKPHLGVNAISGMARVILAIEKDCESL
                     KAKSHPLLGSGTCNIGIVTGGTQVNIVPDECSIEIDRRLLPGEEPRATVEHFREVVKA
                     VPGVDARVDAPMLEDLPLDTRIDERIVLAASAALADLGLSPAPTGVPYGSDASKLAAA
                     GVPSVVFGPGSIDQAHAAVEYVDSSEVEQASEFYRRLMLVFE"
     gene            complement(11596..>12771)
                     /locus_tag="VFQ79_20870"
     CDS             complement(11596..>12771)
                     /locus_tag="VFQ79_20870"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEU0142187.1"
                     /translation="NFGLEAQGMTGAFHRSWGDFGTVRNQAALDYQCFSMLAQANKCS
                     VGDHLHPNGRLNPHTYERIGRTFRSVAAKEPWCEGAKAVTEIASLLSMGARANDSDVG
                     VTRMLTQKRYQFDIVDRESDFSRYRLLILPDGHRLDDPLRDKLAAYLSSGGALLLSHE
                     SGLDMDARQFVLPGMGLDYEGPWKHESQYIEPLEGAREGVPGMVHEMYETGSAVKAQT
                     GTTVLARVWAGYFDKDYRRFQVEQTPYAKPTDYIAAAQRSRLVYFAVPIFRVYAQHGY
                     PVYRDLVANCIGRLIGDPLVKAELPSTAQVTLTEQKGRRILHILHYIPERRSPDLDIV
                     EDVIPLNNVNIAARVPNRPRKVYLAPQRQDLPCEYAEGYARVTVPVVMGHQMVVFES"
BASE COUNT         2436 a         3968 c         3820 g         2547 t
ORIGIN      
        1 tccgaggttt gattatgcgt ccatgggcga cgccatagcc gtgcagcggc tgacttcgtt
       61 tttcggttcg gctggcatat cgcttaaagc cctgcctgcc cacctcgcgg cttgggaatc
      121 gatcaaggac aacaatttaa tcttcatagg cgcctggcga atgcatcccc tcctgcggcg
      181 tctgcctgtc acacaggact ttgaactggg tcccgacgac cagattcata accagaagcc
      241 cggtcccggc gaactgaaaa tttacacgac accttcgcat cgcgactcga tgacctacgc
      301 ttccgtgggt gtctttcccg ggctgaggcc cggacgggag gtgttcgtgg ttaccgcgca
      361 cagttcaccc ggcgcgatgg gcgcagtcga cttcatcacg aacgctggca gcgtgaagct
      421 gatacgggac cgcatcggat tatcttccag cggaccccgg aaatatttcc agatgctgct
      481 ccgagtgtac gtagataatg acgttccgat gaaaacggaa tacgtcaccc accatctgaa
      541 tccgcaaccc cttcggtaac gctaccgtaa caaaacctag ttacgggcgc agaaggaaat
      601 tccgcagcct aagcttgtgc tggaacggtt attatttcat gcggatcatc ttctcacttc
      661 tcgtgtgggc agggccggca ggcgttgcgc ttgcacagtt tccggaaatg cctccgtcaa
      721 cgctcgccgc gccgaaaccg gcgttcgacg acggacagga cgcgatgcgg acgcgcctgt
      781 tccggaccac aacctcgcgc gcgcaaatcg acctcggcgg ctggtgggat ttccttcccg
      841 accccggtga caagggcgaa aagctcaact atggctcacg gcttcccagg cccgaaacca
      901 ggctctgggt gccgggcacc tggaactcgg ttgcccggta ctggaattat gtaggccccg
      961 cctggtatca gagggacttc gagattgccg gcggcgggaa tctgcgtatc atgttcggca
     1021 gcgtcttcta tcgttcgaaa atctggctcg atggcaaact gctgggggaa cacgagggcg
     1081 gctatcttcc gttcgcattc acggtgcgcg atgtgaaaaa aggtccgcac acgctagtgg
     1141 tgcgcgtgga caaccggctc gacgacgcat ctttgccgaa gcagaacgtc gactggtttc
     1201 cttatggggg tatcgaacgt ccggtctacg ccgagatggt accatctgtc ttcatcgaca
     1261 gtttccacgt gatcccctca tcgatccagc cctctcgcgc gagcttgaag gttagagtgt
     1321 tcctatggaa ccttggccag agttccggga ctgtcgagct ggctttcagt gtaaacggaa
     1381 cgcgtttaca tgcggtccga catgctgcgg caagcggaag atcggccgct gaattcgagg
     1441 tcatgctgga gcggccccgg ctatggtcgc ccgcggaccc gaacttgtat tccgcgcgaa
     1501 tatcgctgaa cggcgatgga gacgatcagt tcacccggtt cggcgtacgg gaggtccgcg
     1561 tggatggacc gcgtattctt ctgaacggaa ggccactgaa ggtgatgggt gtgaaccgcc
     1621 acgaagacca tcccgattgg ggttctggca gtccgccgaa tcttctgcgg caggatatcg
     1681 aaatcatcaa gcggctcgga gcgaacgcgg tgcgagccca ttatcccctg ccggaactgt
     1741 tcatggattt ctgcgatcag tacgggctgc tcttcatggc ggaagcgcct gcctggcaat
     1801 actcgtccga gcagttggcg aagcccgaga tacaggaaaa agtcctgcgc cattttgaag
     1861 aaatggtcgc tcgcgatatg aaccacccga gcgttttcac ctggagcctt ggaaatgaat
     1921 ggccgcagcc ggaccgctcc tatgacgtga tccgcgacct gttggcgcgt gcccggcggt
     1981 ttgattccac gcgtcttatc acgctcgtta ccggcgggcc gcacgtccgg agaatacatg
     2041 aactcgtgga cgtgatctgc gtaaattggg cgcgctatca atggtacgat cccttcacga
     2101 ccctcgatgc cgaagaggga gcgaaaagtg tggcggagct ggcaaaaata cacgagcgct
     2161 atcccggcaa gcccgttatt ctgacggaat tcggggggtc ggaggcgcag gctggctggc
     2221 acaactgggg caacgtgaag tggagtgaag aataccaggc cagaaacgtg gaggactccg
     2281 cgtggcatgc actccagcag gactggattt cgggcggctg cgtatggcag ttctgcgaca
     2341 cacgcagcgc gccggagcgg ttcctggcgg gcaggcttca cggctggaac ggcaagggaa
     2401 tcgtcgacgc gaatcgaaac cccaagctgg cgttctacaa gttgcagcag atatttcgtg
     2461 cgatagcgga gcctgcggcc accagtgaag ggacgccatc ttccgatgcc ggaaaataaa
     2521 ggcacaccac aggagaatat actgatgatg ttcaaagttg cggagagttt ctgagatgaa
     2581 aacttcaaat cgtttattcg ccattattct gttgtttgcc tgttgcggct gggcgggcga
     2641 gcgtaaagca aaaaacgtga tcctgtttct tggggatgcg gccggaactt caaccctgca
     2701 tgccgccagt gtccaggagt atggagactc caaccggctg ttcctttacc ggatgcccaa
     2761 catcgcgctt tccgatacct cgtcggcagc ggattgggta accgattcgg ccgcgggaat
     2821 gaccgccatc gtgaccggtg aaaaaacgaa caatggcgtg gtttcgcaat ccgactcggc
     2881 agtgcggggc aagaaggacg gagagccgct caagacgctg cttgaatacg ccgaagagcg
     2941 tgggctttcc accggtgtcg tgtcgaacag ccccatcacg gacgcaactc ccgccgcctg
     3001 ttacgcccat gtcaacgatc gtaaaatgac cggcgaaatt ttcaggcagg cgctgaaccc
     3061 gcgattcggc gatggcgtcg atgtaatgat cgggcccgga cggcaagcca tcctgacggc
     3121 gacgcgtgag cttggaatga acctggaaac ggagatcccc gcgaagggat tccacttcgg
     3181 ggaaacgctg gaccaggtgc cgccggatgc tcgccgggct atcgtgcttc tcggtccggg
     3241 cgaattcgat ttgggtgctg cgacggaact cgcaatccgg atcctctcga aaaacccgaa
     3301 aggattcttc ctgatggtcg aatccgatct ccatacggac aatctgtctc gcggattgac
     3361 cagagccgtt gcattcgaca ggatcattga atccacggcc gcgagcgctt cgaaagacac
     3421 tctcatcctg ttcactgccg atcattcctt cgacatccgg gttggcggcg ccaccaggaa
     3481 aggcctcccg cttgtgacgc aggatgcaga cggcaagcct gtaccggcaa aatccgtgat
     3541 gatcaacggg caccactccg ccgaacaggt cctggtggcc gcgcaaggac ccggcgccag
     3601 ccgcgtccgc ggcgtgatcg ccaataccgg catatttcat atcatcctgt cggcctaccg
     3661 gtggaaaccc gaaccggcac acacgccacc acaaatcact tcgggaaact gacggcggag
     3721 gccagcaatc cggggcagcg cggatcggac ggaatcctcg tgaactatac tagatctcgc
     3781 tatgctccaa gacattcgat tcgggctgcg tatgctcgcc tgcaatccgg gtgtgaccgt
     3841 gttggcgact atcgcgctgg cacttggtat cggcggcagc acggtcgttt tcagtgtagt
     3901 caataccgtc ctgctaaggc ctttacccgt ttccgatccc gaccggctcg tctggatatg
     3961 ggccaataac cccagccgta atctcgccta tgccttcacg gcatacagta gttacgcgga
     4021 gtggaaagcc ggtagccctt cattggaatc catgtccgcg tacgcgcccg tttcggcgac
     4081 cctgctgctc ggcaatgccc cggagcgcgt cgatgttctc aaggtaaacg ccacctactt
     4141 tcccatgctt ggcatccaac ccgtcgtggg ccggaatttc ctagccgaag aggaccagcc
     4201 gggagcccgc agagtcgcga ttctctccta tgatctgtgg gagcggcggt tcggtcgcga
     4261 cccggatgtc atcggccgaa ccataaacct ggagggcgag agcgtcgccg tgacaggcgt
     4321 tttgccgcgt ggttttgcgt tcccgaataa ggtggcggac gtctacgttc ctattgcgca
     4381 cagcaccgcc cgcgcgacgg gtggggctcc accgctgttc gtgggagcat acggacgtct
     4441 caagcccggc gttgctatcg agcgtgctca ggctgagatt gacgtcgttt cacgccggct
     4501 ggaagctgca tatcctggca tgaaaggcat gggggcacag gtttggcgtg tgcgcgactt
     4561 tatggtccgc gacgtccgtc ttagcttgct cgtactgttc ggtgcagtcg gcttggtgct
     4621 tctgattgcc tgcgcgaatg tcgctaatct gctactggtg cgtgccagtg tacgacagcg
     4681 cgagatcgcg ctccggactg cgttgggcgc agggcgctgg cggatcctcc gccagttgct
     4741 tatagagagt gtcatcctca gtgtttgtgg cggtgtcgca gggctcgccc tggccgccgc
     4801 cggaacgcga ctggtgcgac gatccgggcc ggagcaaatc ccgttcctca aagaagtgtc
     4861 actcgacagt cgcgttctcg cgtttgcgat tctcgcgtcc ctactcactg gagttctctt
     4921 tggcctcgca ccggcattgg cggccttccg ggcgcgcgtc tccgagacct tgaaggaagg
     4981 ggccatcgcc gccggcgaga gccgcgcccg caatcggttc cgcggcgcac tggtcgtcat
     5041 cgaggtggcg attgccctgg tcctggctgt cggcgcaacc ctgacgatgc gcaccttact
     5101 acgcttgcag gcgaccagcc caggttttga cgccggaggc gtccttacag cgtcgattac
     5161 gctgccgacg ccaaaatacc cgaaacccga gcagcgcgtc gacttctatc agcgcctcct
     5221 ggatcggacc aatgttatgc ccggcgttca ggccgcaagc atggtaagcc tcatcccgtt
     5281 cggcggctcg gatacgggct ggaacgtgct gatagaaggg gagccggctc cgcggcggtg
     5341 ggacgctccc gtctcctggc gccgcatcgt cgaccctgcc tatttccgcg tgatgcggat
     5401 tcccctcatc cgcggccggg aattcacatc gcatgattca ggctcacccc tggtggctat
     5461 catcaatgag accatggccc ggcgcttctg gcccaacacc gatcccctcg gcaagcgctt
     5521 cggactcacc aagtactggc tcactgtcgt cgggattgtg ggcgacgtga agttcacatc
     5581 gtttacgaaa gatgccgaac cagagttcta cgagccctac cgtcaagccc caggtgcaga
     5641 catgaccctc accgtgcgta cggtctccga tccgttgcgg ctggcccccg cactccgcca
     5701 agctgttctc gaaaccgatc ccacccaggc gatctcgcgc gttaccccga tggcccaata
     5761 cgtctctgat gcggtaggaa caccccgcgt gtcggccctg cttctggcgg cgttcggaac
     5821 cacggcgctc ctgcttgccg ccgttggcat ctatggagtg atttcattct ccgtgacgcg
     5881 ccggactcgt gagatcggcg tccgtatcgc gctcggcgcc gccggacgcg atgtcatgcg
     5941 gatagtggtg ggcgatgcca tcgtgctcgc ttccataggg gtcgcggtcg ggattggcgg
     6001 cgcgctcgcg ctcaacagca tcatgcaacg aatgctgttc ggcgtgaccg caaccgagcc
     6061 attcacctac cttgctgtgt cagccttgct gattgccatc gcggctctgg cggcctatgt
     6121 tccagcacgg cgagctgcgc ggatcagtcc gtctgtcgcc ttgcgctgcg aatgacgttg
     6181 gcattgcgct gcgttgcggg catgcttcgg cgttgactgg aaagcaccat tgccacgcag
     6241 taggcgggat cgtgtccgga tagtgcgtca tccaggaatc gcgcgcagca gtggaatggc
     6301 cgcagatatc tgcggcttag ctggtgaaca acacgactcg ggcacggtgc tcactacgta
     6361 atttctcatc gcacgtgtag aggacggaac cggttgcctc tgccaaggcg atgtaggcgg
     6421 cgtcgtaaac ggtgaaattg tgccgtagtt cccacatacg gccaatcaat ggagtgtgcg
     6481 aataccgctc cgcgggcaaa gccgcgagct ctgctagaaa ttgcctgctg cggtgagagt
     6541 cgattcgctg cctgcgctga gctgttgtgg ccatcctagt gactgcggtc acatccacgc
     6601 gacacctgct agaagtagcg tctattgagg gggaagagaa tgggtagttg gggttgtccg
     6661 cacgaggcga acggcgtctg ccagaaggtg aacaaactcc cctgcgatcc ggggatgagg
     6721 gggtgcgtcc tctacggccg ctttgtcttc agtaatccgg cgaaaaaccg gctggctaaa
     6781 cctgacgccc gaaacacggc ggaagagaag gacggccggc ccagttcgtc cagataaagt
     6841 ttgccatggc atctccgcgc ggttggcagt accctgttag aaccacttgc aacaggaggc
     6901 tccgccacat gaaccgccgt cagtttttcg ctaccgcggc agcaaccggc tcggcttcgt
     6961 ccgctggaac aagcagcggg agtcccgatt tttccgctgt gcgggcggat tttcctcgcg
     7021 cccgaacagc cgcttacctc gataacgcct cgtgccatcc tctcagcatg cacgcggccg
     7081 cggcgcttca ccgctatatc gattgggcca cgaaggaagt gggagagcca tggtggccgg
     7141 gttgggcgca ggcccgcacc gaagcgaaaa cactgttcgc cagactcata aacgcaaaac
     7201 cttcggagat cgcttttgcc cgcagcacca tcgaggcgga gagcaatctg ttgaacggca
     7261 tggcgattcg ggcctcgggc ggcaacgtgg tcaccaacga cctgcactac tccgccgcca
     7321 tctacaacta caggatgcgc cagaaggacg ggctcgatgt gcgcatcgtg aagcatcgcg
     7381 actggcagat cgatatccgc gacatggagc gggctgtcga ccgcaatacg cggctgatcg
     7441 ctatcgcgct ggtctcgaac gtgaacggat acctgcacga tgtccggaag ctcagcgaac
     7501 tggcacactc ccacggcgcc tatctgtacg ccgacatcat acagtgtgcc ggagccattc
     7561 ccatcgacgt tcgtgccatg ggaatcgatt tcgccgcctg ctccacttac aagtggctga
     7621 tgggtgtaaa gggcttcggc ttcctgtatg tccgcgagga tctgcaaggc accgtagtca
     7681 agcccgcaca gcactccggt ggtgtgcgat tcaactatgc gccatgggtg gaacagccgg
     7741 acgccggtct cgacgagatc gctttcagcc ctgtggccgg tccttcgcgc tacgaggtga
     7801 gctatccatc gtacgaagga gccatctgcg cgctggaaag cctgaagtac attcaccggc
     7861 ttggggtcgg gaacattcgc gcgcacgcac gcgcgctgac cggccggcta cagaaggaaa
     7921 tgccggcggc tggatatccc tccatcacgc ccccggacaa cgagagcccg attgtggcct
     7981 tcgccacgaa agatccgcaa cgcgcgatgg aaaaattgag aaaggccggt gttcacgccg
     8041 ccatgcggtt cggcaacaag atgaggattt ctccgtcggt cttcaataac caggaagatg
     8101 tggaccgcct gctggcggcc ctggcgtagc ccccgcctta ctgcgctccg ttgcgcaagg
     8161 ccggcagatc ctcggccagg gtccgtttca gcagaagcag cttttcgtga atcgtcgccg
     8221 gcgtgtagat gtagtcctgc tcccactcgt agccgagccg cgagtccctc cgcgccgcct
     8281 caaaggatgc ctccgtgcgc tcgatctcct cgcgcagaat tgcttccagg cggtccagca
     8341 ccgcagtctt gccggccgcg ttctgcccga gccggaatcg cagatcttcg aattccagca
     8401 gcgcctgctc actacgcatc atgcgctgta gctgttccgc gagcagcgtt tcccggtacg
     8461 cattctgcct ctttgctgcc ggagccgcca gtgcggcttt ccggtagtgc tgcagaccct
     8521 tctccatttc ggcggctgcc agtccaaggt gcttgctgaa aacgggcagc gagaagggcc
     8581 tgacgtaccg ctccgccgca ttcaccttgt ttgtgaaatc cggccaaaga atcaggcggc
     8641 tcggcacgta cggccagtag gggttcaccc aagtggtgcg ggcccaaagc gcgggatccg
     8701 accaggagtg attcaccgtc atcatgcggg gctccggctg tgtcagaaag aatggggccg
     8761 cgacggcatt gttggtgccc caattcggac cggtgtccgg aaccagccga atcgcattgc
     8821 tgaaacactt ccaggcggag agaaccgcgt cttcgttgcc cgctccgaaa tcacggcgtg
     8881 cgatggcccg caggagttcg tcgagcggcg gggcgtcgct ccaggaatac caggcgcgca
     8941 gttcggcaac gaagttcggt ttgaatccat aactccagct ttcgagcacg ccgtccacgc
     9001 cccacttctc gagtgctttg tagcgttcat accactggta gggaaacggc agatagggga
     9061 aagtgccgta ttgccagctc gcgaaggtgt ccgctttcgt atagacgccg cggaacccga
     9121 gtttcttggc ttccgcgatc tgtgcctgtg ttacttcggc cggtcccacc tggttgatcg
     9181 catagtcctt caaccagccg cgctccccgt ccagttcgaa cgccttgccg ttttcaaacg
     9241 tcagcagcgg aatggaatcg cgcggcaatt gcgccgtacc gtagcgcttg ttctccgcct
     9301 ggtgtgggcg aaagtccacg ttgtactccc agggcaaaac ttcgatagcg ggattgatct
     9361 tgcggcactc ttccgccaca atggcgaccg cgcgggccca ctcgcgaagc catttgcgga
     9421 ggtcctggtc gccttgcccg cccgcgccga accacctttc gtagtagaag ccttcagtca
     9481 acagaacata gccgcgaatg tccgggaact ccgttacgat gtcgcgcacc agtttgcgca
     9541 agccttcctc gttctccggc gtgggcgtgt agcgccacag gatgggcacg tagacgccga
     9601 tgccgtattt cgcggcgcgc cggaccaggt ctgccatcgc gccgggcggg tgctccttca
     9661 tcttgtccca gtgcggtccc acgcccggcg cgttgttcgg gtttcgataa atcgaatcgt
     9721 gaatcgcgtc gaatccgtac cggacgagcg tcgccagaaa ccggtccggc cagttcatcc
     9781 aaccgagacc ggacaacgtc atgcgcgtgc gatagaggct atggcgcgtg gtgtccaacg
     9841 tgcgcgaaag gaacggagct tcgcgcaagc tgaaacgcgc ttccacgttg tacagcccgt
     9901 acatggcgcc gcgctcgtca aaaccgcaca cggcgatgcg ctcacccttc acctgaatct
     9961 ggtaatcttt cgggcctttc agcgcctgtc cgcagccggg catctggtcg cgagtgcccg
    10021 ccactaccga acggctcatg gccgcccagc catcgagcgt acccggagtt tccagcttca
    10081 ggttcacctg catcccgtct gcgatccgtt cgcgaaattc gacggcggcg tggcgcagca
    10141 gcggtcctga atccgaccgg atgacgaggg accatccctg cggcggaatc gcgatttccg
    10201 tcgcagccgg acgggccgcc gggtcgcggc gcaacggttc cacgcgctct ccgaggactt
    10261 tctgaaacgt gtaagacttc tccaccggca aggcgggcaa cgctttttcc gcgaactcgg
    10321 ccgcactcgg cccgtcctgg gccgacgcca cgctgaaagc aagcgctgcc gcgcatgcag
    10381 tacgcaggaa cacttctgtc atattcgctt cctcatgcgc gcggctcagc tactggggtc
    10441 attcgaaaac cagcatgagg cgccggtaga attcagatgc ctgctccacc tcgctcgaat
    10501 ccacatactc caccgcggca tgcgcctggt cgatgctgcc gggtccgaac actacgctgg
    10561 gcacgcccgc ggccgcgagc ttgctggcat cgcttccata tggcacgccg gtgggcgcgg
    10621 gactaaggcc aagatccgca agggccgccg aggcggcaag cacgattctt tcatcgatgc
    10681 gcgtgtcgag cggaaggtct tcgagcatcg gcgcatcgac ccgggcatcg actcccggca
    10741 ctgccttcac cacttcgcgg aagtgctcga ccgtcgcgcg tggctcttca ccgggcagca
    10801 gtcgccggtc gatctcaatc gaacattcgt ccggcacaat gttcacctgc gttcccccgg
    10861 tgacgatccc gatgttacag gttcccgagc caagcagcgg atgacttttc gctttcaagc
    10921 tctcgcaatc tttctcgatg gccaggatca cacgggccat gccggaaatc gcgtttaccc
    10981 cgagatgcgg tttggaactg tgggccgctt tgcctcgcgt atgaaccttg aagcgcacgc
    11041 atcctttcgt cgccgtcacc aggcgcatac tggtcggctc ggctacgacc gcgccatgcg
    11101 cggtgagccc ttcgcacaat ttcacaaccc cgcggtaaga gtactcttcg tcggcggcag
    11161 ccactaacca cacctcgcaa ggcggctttc tgccgccctc ggccaccgcc gcgagcgcgt
    11221 gcatcatggc ggccagcccg cctttcgtgt cgcacgcgcc gcgtccgtac aacctgccac
    11281 cttcaacgcg cggctcgaag ggcggaatgg tcatgccttt caccgaagcg gtgtcggtgt
    11341 gcgcttcgag aatcaggcgg cgcgaggact gacgtcccgg cacagccgcg atcaggttgg
    11401 gccgccccgg aaacacctcc tggcgccagg tgcggatcga gcgtttggca aaaaactgtt
    11461 cgatatattc gacgagcccc gcctccgagg ttccactctc gtaagccggg ttgatgctgt
    11521 tgatccggac cagtccggcc agcgtttcca ctacgggaga aagtgccatc gctcgctaca
    11581 tcataccggg cgccgctagc tttcgaacac caccatctga tggcccatga cgaccggcac
    11641 agtcacacga gcgtagcctt ccgcgtattc acacggcagg tcctgccgct ggggagccag
    11701 gtagaccttg cggggacggt tgggcacgcg cgccgctatg tttacattgt tcagtggaat
    11761 gacatcctcg acgatatcga gatccgggct gcggcgttcc ggaatgtaat ggaggatgtg
    11821 cagaatgcgg cggcctttct gctcggtcaa cgtcacttgc gccgtcgatg gcaattccgc
    11881 cttcaccagc ggatcgccga tcagccgccc gatgcaattg gctaccaggt cgcggtatac
    11941 cggatacccg tgctgcgcat agacgcggaa gatcggcacg gcaaagtaca ccagcctgct
    12001 acgctgcgcc gccgcaatgt aatcggtcgg cttggcatag ggcgtctgtt ccacctggaa
    12061 gcgccggtaa tccttatcga aatagcctgc ccacacccgc gccagaaccg tggtgcccgt
    12121 ctgcgccttc accgccgaac cggtttcata catctcgtga accatgccgg gcacgccttc
    12181 gcgcgcgcct tcgagcggtt caatgtattg cgattcgtgc ttccagggac cttcataatc
    12241 gagccccatg cccggcagca caaactgccg cgcgtccatg tccaaacccg actcgtggct
    12301 gagcagcagg gcgccacccg aggaaagata cgccgccagc ttgtcacgca gcggatcgtc
    12361 cagccggtga ccgtccggaa gaatcagcag gcgatagcgc gagaaatcgg actcgcggtc
    12421 gacgatgtcg aactggtatc gcttctgcgt gagcatacgg gtgacaccga cgtcggagtc
    12481 gttcgctcgc gcgcccatgc tgagcagaga agcgatctcc gttacggctt tggcgccttc
    12541 gcaccagggc tccttcgccg ccaccgaacg gaaggtgcgt ccgatgcgtt cgtacgtgtg
    12601 cggattgagc cgcccgttgg ggtgcagatg atcgcccacg gaacacttgt tcgcctgcgc
    12661 cagcatgctg aagcactggt agtcgagcgc cgcctggttg cgcaccgtgc caaagtcacc
    12721 ccagctacga tgaaacgccc cggtcatgcc ctgcgcttcc aatccgaaat t
//
LOCUS       DASTMK010000504         4934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_217051, whole genome shotgun sequence.
ACCESSION   DASTMK010000504 DASTMK010000000
VERSION     DASTMK010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217051"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFQ79_20875"
     CDS             complement(<1..316)
                     /locus_tag="VFQ79_20875"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU0142188.1"
                     /translation="MTLPLGVILLILFALFSAGSLCSEEPRRDVIVVTGTFDPVPLEE
                     SERPVTVLDIARLRLVSNSLASLLRLEPSLDVRERSPNGVQADVSIRGSTFGQTLVLL
                     NGL"
     gene            349..2163
                     /locus_tag="VFQ79_20880"
     CDS             349..2163
                     /locus_tag="VFQ79_20880"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU0142189.1"
                     /translation="MGKVLLGLLASSVLFAQIVIEFRPVATGVDAPSDIQHAGDGTNR
                     LFFVQQNGLVRVHRGGALLSTPFLDIRSRVRAGGERGLLGLAFPPDYAQKQRFYVNYT
                     DLNGHTVIARYRVGPNADTADASTEQILLRIEQPYANHNGGQLRFGPDGYLYIGMGDG
                     GSGGDPQNRAQNPRERLGKMLRIDVESAPGTFQIPPSNPFAGSTTHDPAIWALGLRNP
                     WRFSFDRGTGDLWIADVGQNRYEEINFQPASSRGGENYGWNIMEGLHCFSTGTCSQQG
                     LTLPVAEYGRADGCSVTGGFVYRGSAFPGLRGTYLYGDYCSGRIWGIRREGSQWANQL
                     LATTGFSITTFGEDEAGELYVADAARGTIHRIEGPRAPVFSAESVVNAASFQAGMTPG
                     SLTTLFASGIMDSPGIAAASTIPLPLSLNGVSVTIGSVAAPIHALANVDGQEQINFQV
                     PFEIAGRQTAPVVITREGASSLPVDVPVLDVQPGIFTSASGDAIAIHNANNTLVTAAA
                     PLVAGEYIYVYATGLGPVANNPGTGDAAGASSETQSGVEATLGGAPAEVQFAGLAPGF
                     VGVYQINLRVPAGLPPGTAELTVRTGGIGSPPVRVPIQ"
     gene            2184..3362
                     /locus_tag="VFQ79_20885"
     CDS             2184..3362
                     /locus_tag="VFQ79_20885"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142190.1"
                     /translation="MQPNRREFLKPLAAVPLFVPRTARGANDRLNYGVIGTGSRARWL
                     NRTFQKLGAQCVALCDVYDRHLDAARDESPKDAKGYVDYKELLGHKDLDFVVIGTPDH
                     QHCPNLLAALDAGKDVYLEKPLSLSLDESRRMVDAVRQSDRVVQIGMQRRSMPFIRKA
                     KQLIDDGALGKVSMVKALWNWHFTMPLDNSPLPGKLDWERFVGPAPKREFEPKLFRWW
                     RGFWDYSGGNMTDQGTHLMDVVQWMTNAGPPRSAVCQGYIANAKAGEVPDVFSAAFEY
                     PDLMATWTLNYTSAYEHDWSIQFQGEKATMMLDRLGYRIYKDPGASPQPWSQTARPEL
                     IAQEADRDSAEAHQQNFLDCVRSRKQPNCTVEIAAAAVAGPHLANLAYRENRKVMAAG
                     "
     gene            complement(4081..4734)
                     /locus_tag="VFQ79_20890"
     CDS             complement(4081..4734)
                     /locus_tag="VFQ79_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142191.1"
                     /translation="MISIRSTVVAAFMGLAFTWTATAQVNFGLAGGLAGSATKEGLAG
                     YTATGFGRSASYGGGGGYRFRNGLIVDGRFEQLQMRLSEDGADMGSLRLRPAVITVGY
                     QGIPAKGRGFSGHAYVGGGVAFSSFRKGNAITALERQYGGQMVVETKSAPALIVGAGI
                     DYFLSPRVSLTSDFRFVGANVGTSWAAVGPGGRAALPFEKFFVSNGQVVGGMKFWLK"
BASE COUNT         1064 a         1518 c         1427 g          925 t
ORIGIN      
        1 gcagaccatt cagcagcacc agcgtttgac caaacgtgct cccacgtatc gatacatccg
       61 cctgtactcc attcgggctt cgctcgcgaa catccagaga cggctcaagc cgcaacagac
      121 tcgccaggga gttcgaaacc agccggaggc gcgcgatatc caaaaccgtc acgggccgct
      181 cgctttcctc aagcggcacc ggatcgaaag ttcctgtcac cacgattaca tcgcggcgcg
      241 gctcctcgga gcacaaggat ccggccgaaa agagcgcgaa caaaattaac aggatgacac
      301 ccagtggcaa tgtcacagag taacatttgt cgaactacac tcgaaattat gggcaaagtg
      361 ctactggggc tattggcgtc atccgttctg ttcgcgcaaa tagtgatcga attccgtccg
      421 gtggcgaccg gagtggatgc acccagcgat attcaacacg ccggcgatgg aacgaaccgg
      481 ctgtttttcg tccagcaaaa tggcctggtt cgcgtgcaca gaggcggcgc ccttctgagt
      541 acgccgtttc tcgatatccg ctcgcgtgta cgtgccggag gcgagcgcgg tcttctgggc
      601 cttgctttcc ctccggatta tgcacaaaag cagcgcttct atgtgaacta caccgacctc
      661 aacggccaca cagtgatcgc gcgctaccgt gtgggcccga atgccgatac agccgatgcg
      721 tctacagaac aaatccttct tcgaatcgaa cagccgtacg ccaaccacaa tggaggccaa
      781 ttgcgtttcg ggccggacgg ctacctttac atcggcatgg gagatggcgg tagcggcggc
      841 gatcctcaga accgggcgca gaatccccgc gagcggctcg ggaaaatgct gcgcattgac
      901 gtggagtccg caccaggcac gtttcagatt cctccgtcca atccgtttgc cggcagcacc
      961 actcacgatc ccgctatttg ggctttgggc ttgcgaaatc cgtggcgctt ctcctttgac
     1021 cgcgggacag gcgatctctg gatcgccgac gtgggtcaga accggtacga ggagattaat
     1081 ttccaacccg cctccagccg tgggggtgaa aattacgggt ggaacatcat ggagggcctg
     1141 cactgcttta gcacaggcac ttgcagccag cagggactca cgctcccggt ggccgagtac
     1201 ggccgcgcgg acggctgctc cgtcaccggg ggatttgtgt accgcggcag cgcatttccg
     1261 ggcctgcgag gcacttacct ttatggcgac tattgcagcg gaagaatctg gggaatccgg
     1321 cgggagggga gccagtgggc caatcagctt ttggccacaa caggtttcag cattacgacc
     1381 ttcggcgagg acgaagccgg agagttgtac gtggccgatg cggcacgcgg cacgatccac
     1441 cggatcgaag gtccgcgtgc gccggttttc agtgccgaat ccgtggtgaa tgcggccagc
     1501 tttcaagcag gcatgacgcc cggatcactc accaccctgt ttgcgtccgg catcatggac
     1561 tcgcctggaa tcgcagccgc aagtacgatt ccgctgcctc tctcattgaa cggagtcagc
     1621 gtgacgatcg gcagcgtggc cgcacccatc cacgctcttg caaacgtgga tggacaggag
     1681 cagatcaact ttcaggttcc cttcgaaatc gccggccgcc agaccgcgcc ggttgtcatt
     1741 acaagggaag gggcgtccag tctgcccgta gatgtaccgg tcctcgacgt ccagcccggc
     1801 atctttactt ccgcgagcgg cgatgcgatc gcgatccata atgcgaacaa cacgctggtg
     1861 acggctgccg ctccgctggt agcgggcgaa tacatctatg tttacgcgac aggtctcgga
     1921 cctgtggcga acaacccggg aacaggggac gccgcgggag cctcatcgga aacgcagtcc
     1981 ggggttgaag ccacccttgg cggcgcgccg gccgaggtgc aattcgctgg acttgcgccg
     2041 ggattcgttg gcgtctacca gatcaacctc cgtgttcccg cggggttgcc cccgggtacg
     2101 gccgagttga cggtccgcac cggtggcatt ggcagccccc cggtgcgggt tccgattcag
     2161 tagtctggtg taggagtctc gtcatgcaac cgaaccgaag agagttcctg aaacctctcg
     2221 ccgcagttcc gctgttcgtt ccgcgcacgg cgcgcggcgc caatgaccgg cttaactatg
     2281 gcgtcatcgg caccggcagc cgcgcccggt ggctgaatcg cacgttccag aaactcgggg
     2341 cgcagtgcgt ggccctctgc gatgtttatg accggcatct cgacgccgcc cgcgacgaat
     2401 cgccaaagga tgcgaaaggc tacgtcgatt acaaggaatt actgggacac aaagacctgg
     2461 atttcgtcgt gatcggcaca cccgaccacc agcactgtcc gaatctgctg gccgctctcg
     2521 acgcgggcaa agacgtgtat ctcgaaaagc cgctgtccct ctcgctcgac gagagccggc
     2581 gaatggtgga cgccgtccgg cagtccgacc gcgtcgtgca aatcggaatg cagcgccgca
     2641 gcatgccttt catccggaaa gccaaacaac tcatcgacga tggcgcgttg ggcaaggtgt
     2701 cgatggtaaa ggcgctgtgg aactggcatt tcaccatgcc gctcgacaac tcaccgcttc
     2761 cgggcaagct ggattgggag cgcttcgtgg gacccgcacc gaagcgtgag tttgaaccca
     2821 agctgttccg ttggtggcgt ggcttctggg attattccgg agggaacatg acggaccagg
     2881 gcacgcacct gatggacgtg gtgcagtgga tgaccaacgc gggtccaccg cgttccgccg
     2941 tatgccaggg atatatcgcg aacgccaaag cgggtgaagt ccccgatgtg ttcagcgcgg
     3001 cgttcgaata tcccgatctg atggctacct ggacgctgaa ttacaccagc gcgtacgagc
     3061 acgactggtc cattcagttt cagggcgaaa aggccacgat gatgctcgat cgcctgggat
     3121 accggatcta caaggaccct ggcgcttctc ctcaaccgtg gtcgcagacc gcccgtcccg
     3181 aactgatcgc ccaggaagcg gaccgcgaca gcgccgaggc gcaccagcag aattttctgg
     3241 attgcgtcag gagccgcaag cagccgaact gtacggtgga gatcgccgcc gccgcagtcg
     3301 ccgggccgca cctggcgaac cttgcatacc gcgagaatcg caaggtcatg gcggcggggt
     3361 agggtcccgt tttctcgcag aataccctgc cgggaggttc actcgccccg tgcgaaaccc
     3421 aaacactatc cgacatgctt tcctgcccac accagttatc ttctacgacc tttgcactgg
     3481 atcagaaccg gggtcgaggg cagcgatgag atcgcccaag acacggatcg atagaatccc
     3541 cggccgcaac acgggccaag tcacattggc aactacaagc agcaatacgt gggtgtagaa
     3601 cggcaacttt gggatcgcac cggccatcgt aagcaggccc actatcgcaa catatgcgat
     3661 cagaacgcta atggagggtt tttggagggt gtggggcggg ggcctcagct cggtttaagc
     3721 ctgatgatcg tctccccggc acttgtcaat atagttccac tatggataga tattgacaga
     3781 tcctcagttg ctcgcatggg aagaatcagg ctatgccgcg ctcgcgtttc gacgaactcg
     3841 taaacctcgc agaagaacac gatagactgg tcaccgcgga gcaggcccgc tcggccgtcc
     3901 acgaaagttg acaaggcgcc cattccgatt ctgacgaaag agatcgcccc ccgaacacgc
     3961 tcccgccgcc aaacgaaaca cggcgaaacc tgagggctgt catagtgatg tcttgacgac
     4021 gaccgaaaac tacggagcaa ggccaccgca atccattcgc ctgcggtggc taggattatt
     4081 tcatttcagc cagaacttca tgcccccaac aacttgaccg ttactgacga agaacttctc
     4141 gaacggcaac gcggccctac cgccaggtcc gacagcggcc cagctggtac cgacattcgc
     4201 accaacgaaa cggaaatcgg aggtaaggct aacccgagga gagagaaagt aatcgatccc
     4261 agcgccgacg atcaaggccg gcgcactctt ggtttcgacc accatctgcc caccatactg
     4321 ccgttcaagc gcggtgatgg cattgccctt cctaaaactc gaaaaggcaa cgccaccccc
     4381 cacgtacgcg tgcccggaga aaccgcgacc cttcgccgga atgccttgat agccgacggt
     4441 gattactgca ggtcggagcc gaagcgaacc catgtccgcc ccatcctctg aaagcctcat
     4501 ctgaagttgt tcgaagcgtc cgtcaactat aaggccgttg cggaagcgat agcctccccc
     4561 gccgccgtaa gatgccgagc gcccgaatcc tgttgcagtg tagccggcaa gtccctcctt
     4621 cgttgcacta ccggcaaggc cgcccgcgag gccgaagtta acctgagcag tagcggtcca
     4681 agtgaacgct aagcccatga aagctgcaac taccgtggat ctgatcgaaa tcatcaaagt
     4741 ctcctttttc taagtgagct tccaatgcgg tgcggccggg cctgggccgc gtataccgga
     4801 tgaaggattt cccgaaactg gggtaatcac tctccccatt tcctttgact ctctttcgcc
     4861 aggacaggct gccatcggta ttcaatcacg gatcggcgag gcaatgcagg gatcatccta
     4921 ctctcaaccc tccg
//
LOCUS       DASTMK010000505         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_282454, whole genome shotgun sequence.
ACCESSION   DASTMK010000505 DASTMK010000000
VERSION     DASTMK010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282454"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFQ79_20895"
     CDS             complement(<1..539)
                     /locus_tag="VFQ79_20895"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEU0142192.1"
                     /translation="MKKSGESSKQQSGKTHGNKTAGLGRNAALAKPSVSQLPSSIGNH
                     AFTQLVEGRSSQPAQDPHAALITRAAQDPGSPLEHPLRQNLERQLGADLSNVRVHQGS
                     GSAAAAESLSARAYTIGSDIYFGAEAGRLDEAARGRLLAHEAIHSVQQGGRPVPLTGT
                     LQVSRPSDAAEVQARTAAES"
     gene            complement(540..2672)
                     /locus_tag="VFQ79_20900"
     CDS             complement(540..2672)
                     /locus_tag="VFQ79_20900"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0142193.1"
                     /translation="MTGASGVETGIAQGSLLPMLQELDRLLESAVAAAPAFYGWDAGR
                     DAFRGLYINEQDARRTLLRGPCFPLPAAAPDRGDAWLQHGPMARLAERYDLDAFDCGV
                     LLLALAPEIDVRYERVYAYLQDDVTRRRPAVDLVLNLLCDTAEEKIERRRHFEPQASL
                     IRHKLIELLADPAQPRPPLLAHYIKLDEIAAGLLLDRDGLDSRLAAFCAFSRAEISLA
                     SLPLERAARDGLFDAAQQAHRSGRPARFYFRGPDLPLRKLTAEALSYDLGARLLLADL
                     GRFEDNPLEAIGALFRYAANHGVMLFLDHADSLAFDSGELRYLTESLAASSCDAILAG
                     AAAWPAGRLFDDAGIVEIEFGTPAFKIRREQWRSNLSVQGCDLPDAQLDSLADLFHLH
                     SDQIAAASVAARNRAWQRGSEQPSLEDCFRAARAQSGQALATLTRKVEPLYGWADIVL
                     PEPTLAQLRDVCNRVRRRRQVFHEWGFDSKLSLGKGVTALFAGPSGVGKTMAAEIIAR
                     ELALDLYKIDLAGVVSKYIGETEKNLDRIFAAAESSDAILFFDEADALFGKRSEVRDS
                     HDRYANIEISYLLQKMEAYRGVAILASNLRQHLDESFLRRLAFTIHFPFPDEAHRRGI
                     WKSVWPQAAPLDSAVDFDFLASRFKLSGGNIRNAALSAAFLAAEEGGTIAMRHLLRGV
                     EREYQKMGKPLGRDQFQPYSIGESTREA"
     gene            complement(2673..2918)
                     /locus_tag="VFQ79_20905"
     CDS             complement(2673..2918)
                     /locus_tag="VFQ79_20905"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEU0142194.1"
                     /translation="MELERDDERKEARYANLFRVGFNALEFLLVFGQQFDDSEEERIH
                     TRIVISPLHAKAFCELLESSLREYERTFQPISDPEQK"
BASE COUNT          628 a         1012 c          975 g          623 t
ORIGIN      
        1 gactcggcgg ccgttctcgc ctgtacttcg gcggcatcgg atgggcggct cacttggagg
       61 gtgccggtga ggggcacggg ccttccaccc tgctgcacgc tgtgaatggc ttcatgcgca
      121 agcagcctgc cccgcgccgc ctcgtccaac cggccggctt ccgctccaaa atagatatcg
      181 ctgccgatcg tgtaagctcg cgcactcaga ctttccgccg ctgccgccga acctgatccc
      241 tggtgcacgc ggacattgct caggtccgcc ccgagttgcc gttccaggtt ctgccgcaaa
      301 ggatgctcga gcggagagcc cgggtcttgt gcggcccgcg tgatgagcgc ggcatgcgga
      361 tcctgcgccg gctgggaaga acgcccttcg accaactgag tgaaggcatg gtttccaatg
      421 gaggacggca gttgggaaac cgacggcttt gccagcgccg cattgcgccc aagcccggcc
      481 gttttgtttc cgtgcgtctt cccgctctgt tgctttgagg attcgcccga cttcttcact
      541 tacgcctccc tcgtgctctc gccgatcgaa taaggctgga actgatcgcg tccgagcggc
      601 ttgcccatct tctggtactc ccgctcgact cctctgagca ggtgccgcat cgcgatcgtg
      661 ccgccctctt cggccgccag aaacgccgcc gataatgcag cgtttcgtat gtttccaccg
      721 ctcagcttga aacgtgaggc gaggaaatcg aaatccaccg cgctgtccaa gggtgcggcc
      781 tgcggccaca cgctcttcca aatcccgcgc cggtgcgctt catccggaaa cggaaaatgg
      841 atcgtgaaag ccagccgcct caggaacgat tcgtccaggt gctgccgcag gttggaagcg
      901 agaatggcaa caccccggta ggcctccatc ttctggagca ggtagctgat ttcgatgttg
      961 gcgtaacggt cgtgcgagtc cctcacttcg gaacgtttgc cgaacagcgc atcggcttcg
     1021 tcgaagaaaa gaatcgcgtc ggagctttcg gccgcggcga agatgcggtc gagattcttt
     1081 tccgtttcgc cgatgtattt gctgaccaca ccggctaggt cgatcttata cagatccagc
     1141 gccagttccc gggcaataat ttcggcggcc atcgtcttgc ccactccgct cggtccggca
     1201 aacagcgcgg tcacgccttt gccgagcgac agcttgctgt caaaacccca ctcgtgaaac
     1261 acctgacggc ggcggcgcac gcgattgcac acgtcccgaa gctgcgccag agtgggttcg
     1321 ggcagcacga tgtcggccca gccatagagt ggctcgacct tgcgcgtgag cgttgccagc
     1381 gcctggcccg actgcgcgcg agccgcccgg aaacagtctt cgagcgaggg ctgttcgctg
     1441 ccccgttgcc aggccctgtt gcgcgcggca acggaggcgg cggcgatctg atcggaatgt
     1501 aaatggaaaa gatcggccag cgaatccaac tgcgcatccg gcaaatcgca tccctgcacg
     1561 gataaattcg agcgccactg ctcgcgccgg attttgaacg ccggtgttcc gaattcgatt
     1621 tcgacgatgc cggcatcgtc gaataagcgg ccggcgggcc atgccgcagc acccgccaga
     1681 atggcatcgc acgaactggc ggccagagat tccgtcagat accgcaattc gccgctatcg
     1741 aatgcgaggg aatcggcgtg atccagaaac agcatgacgc cgtgattcgc agcgtagcgg
     1801 aacaacgcac cgattgcttc caggggattg tcttcgaagc ggcccaggtc cgccagaagc
     1861 aggcgcgcgc cgaggtcgta agacaatgct tccgcggtga gctttcgcag cggaagatcc
     1921 ggtccgcgaa agtagaatcg cgccggccgt ccggaccggt gggcctgctg cgcggcgtca
     1981 aacaggccat cccgagcggc ccgttcaagc ggaagcgagg ccagggagat ttccgctcgt
     2041 gaaaaagcgc agaaggccgc cagacgcgag tccagtccgt cgcgatcgag aagcaggccg
     2101 gcggcgatct catccagctt gatgtaatgc gcaagcagcg gcggacgcgg ctgggcggga
     2161 tcggccaata gttcgatcaa cttgtgccgg atcaggctgg cttgcggctc gaagtgccgc
     2221 ctgcgctcaa ttttctcttc cgccgtatcg cacaggaggt tcagaacaag gtcaactgcc
     2281 gggcggcggc gggtcacgtc gtcctgaaga tatgcgtaca ctcgctcgta acgcacgtca
     2341 atttcaggcg ccagagccaa cagcagaaca ccgcaatcga aagcgtcgag atcgtatcgt
     2401 tccgccaacc gcgccattgg cccgtgctgg agccacgcat caccgcgatc gggcgccgct
     2461 gcgggcagcg gaaaacacgg accgcgcagc agcgttcgcc gggcgtcctg ctcgttgata
     2521 tagaggcccc gaaacgcatc ccgtcccgcg tcccatccat aaaaggcggg agcggccgct
     2581 acggcgcttt cgagcaggcg gtcaagttcc tgcaacatcg gaagcaaact cccctgtgcg
     2641 atcccggttt ccacaccgct cgcgcctgtc atttatttct gctccggatc ggagatcggc
     2701 tggaacgtgc gttcatattc gcgaagcgac gattcaagaa gttcacaaaa cgctttggca
     2761 tgaaggggac tgatgacgat tctggtgtga atgcgctcct cttcggaatc gtcaaactgc
     2821 tgaccgaaaa ccagcaggaa ttcgagggca ttgaagccca ctcgaaacag gttggcgtac
     2881 cgtgcttcct tccgctcgtc atcgcgctcg agttccattc atagcccgga tacaggaatt
     2941 gcgacggaga atgcaccttt cccgccacga tgggagccgg agacgtgctt cccctgcaaa
     3001 tgccggtaac caggaaagat ctaacttgtt caccgaagtg cgggtgcgtc tctcaggccg
     3061 gctgttgtac cggtttgtga caaccggttg tatcagcccc tgtcaccggt tttagcggaa
     3121 tgtgacaact ctgctccggg cttcacgatg tgatatttcg ccatcttccg gtacagtgtc
     3181 atgcgcgacc agtgcaggct gcgtgcggcc tcgctaagat tccagcgggt ggaaaaca
//
LOCUS       DASTMK010000506        11436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_314438, whole genome shotgun sequence.
ACCESSION   DASTMK010000506 DASTMK010000000
VERSION     DASTMK010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11436
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314438"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..2353
                     /locus_tag="VFQ79_20910"
     CDS             230..2353
                     /locus_tag="VFQ79_20910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEU0142195.1"
                     /translation="MFWENVVRVIDNTIGVANLSGGDETALDDAIYDSDNSLSLAIIL
                     SALEWDGGSVTTWNGTSGDDTYTGGAGMDTLNGGYGIDTLNGSTGADRINGESGNDIL
                     DGQSGDDFVQGGAGNDTYKYTAGHGWDTFEEYGTDTGDKILFASGVAIGDLTISRVSS
                     YDMLIEVASGVGGGQILIRNQFNTNQRIESLEFAGGGSPYNLLTQTAWTLTGTDGDDQ
                     LWGVWGAGYQDTIYGGDGDDTIYAYAANHTDNGANFLYGEDGDDILDAYYSSGADTLR
                     GGEGNDTATGGGGSDIYIYDTGHDTYNEVSGTADEIQLAAGIVSGDITYARIRAYDLL
                     IEVAGEGSITIKDFYYSAGYRIETLRFSDTSTVNLVNVTPTSLKGTQGADTIGGANSF
                     DDVIYGFGGNDQLSGNSGNDTLYGGDGNDTLYGGLGNDYNEGGAGNDTMEDTSGGNDT
                     YVYSGGLDSVYDNTGTEVLRLTGPWTVNDLAFSDLGSHHKITITASTHEIQLLWVDYS
                     SAYLIETIEFADGFTTSLNTWKTWNNGTGSGETLNGTASADTIIGFSGNDTLNGSDGN
                     DAMHGGADDDTLNGGNGADLLHGGTGADTLNGNDGLDTLYGGDGADIFLFDVSAFNNI
                     DNVKDFNVGQNDALDIADILTGYSGVEDINEWVSFTNSGGNTQVFVDRDGTGGTYSAA
                     QIALLHGVVDLDADDLLMNGNLIAV"
     gene            2380..3186
                     /locus_tag="VFQ79_20915"
     CDS             2380..3186
                     /locus_tag="VFQ79_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142196.1"
                     /translation="MQQEKPKKRKSFLRRNFDFLLILLVLLPLFYLYGLFYPVSPFEP
                     REESFAPYERHYHFSFRFPFVTSYFLMVPENYQPEKHTYPLVLMLHGASRHMYGGKVL
                     ARPIMRENLPFFAVIPIMPFGYTWASPKTFSLRPQALPLAMSTMRSVMKNYSINENQV
                     YVTGYSLGGIGTYAAVTRYPGVFAAAAPTDAYWNPETADRIDDVPIWIFHGVQDQGMP
                     VAYARMLAAGLQQLGRDVRYTEYANRGHGAWIPAYENPDFWMWLIRQQKH"
     gene            3831..4892
                     /locus_tag="VFQ79_20920"
     CDS             3831..4892
                     /locus_tag="VFQ79_20920"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU0142197.1"
                     /translation="MTARKKTPGRLKRKHRTTAIDLFSGCGGLSLGLKSAGFDVIAAI
                     DNDPLACATYRMNHKDTVLIEKDISKTKPTYLRKRLGLRPGQLDLLAGCPPCQGFSTL
                     RTLNGKKNVKEPMNDLVFQFMKYVRAFRPKAIMMENVPGLAKDSRLEELIRDLNRLGY
                     RCEARVLNAADFGVPQRRARMILIAMKRRTPVFAQQARVSRSVRSAIGALPRAGTGSD
                     PLHDYEVNRAEHVVELIKRIPTNGGSRRDLPESDQLDCHKKCDGFADIYGRMAWEKPA
                     PTITGGCINPSKGRFLHPRQHRAITLREAALLQGFPKRYKFDLSAGRFNAAQMIGNAF
                     PPEFAERHAKVIRKQLVEQ"
     gene            4911..7853
                     /locus_tag="VFQ79_20925"
     CDS             4911..7853
                     /locus_tag="VFQ79_20925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEU0142198.1"
                     /translation="MTVAEDALRANLNDEQYRAAIDPSAQVLTLACAGSGKSRTLAYR
                     IAWLIADRGATPEGIVAFTFTEKAADSIKLRVSAALAACELDTNLLGRMYIGTIHSYC
                     QYLLGRADARYRQFEVLDDNRLKLYLIEKYGLLQLNRFRPRANDRYFAVIGQCHDAWT
                     HHNEEMLDPATIEPHDPDLAAYCRDLRERLIADQFLDFSLMQRLVVDALQNRDPGILD
                     SVRQLQHLLVDEYQDINPIQEALIGLLHQNCQTLFVVGDDDQSIYGWRGADVTRIQTF
                     TDRFPAASTHTLARNYRSTPLIVSNADAFAHAELGANRIVKNPQADDTDQPNEFRNLW
                     FGTRAEEADWVANKIASLLGTAYREPNGTVRGLTPADFAILMRSTRTAETGGNPPRHV
                     PFTSRLSDRGILFSLEAGGGLFDRPQVQLLRAAFELLRNGQPDRNIVRQFFDQQVTPL
                     FPRTDFNDLTGLFATWGRDIHAPTDRGVPRRRIYPQKLVFDLLNTFDVANTPLDDAIL
                     QDIGVFSRIIQDVEAVYPSVDSTMRFTSILNFLNVVAEDGYDTSSSDIVQRPDAVTVS
                     TVHKMKGLEFPVVFVVDVENQRFPGKRRNYGGWLPAATIQDSLNRGAYQSRREDEARL
                     FYTALTRAERFLYVTGSANAPGWGRPKRPSPFAAHLADARIVTDATAATAGLVNAPPA
                     RRIEASNLPTSYSDIRYYLRCPKDYHYRRIFGFSPSIPDLFGFGMTVHASIGKLHQQF
                     PAQRPTAAQAEAVVQDIFHLKHVQQSNDPVGRPGPYERARTRAQELVSGYAQQFSTDF
                     ERRREVEARFEIPVNGAVVSGAIDLMLEEDENGNVVDACVVDFKTLGGGDDPLENADL
                     EWTELALQVQLYAKAARDVLGHVADEGFVHLLKDGQRLQVPVGDAAVGAALANVEWAV
                     DRIVASDFPMRPHRTKCEDCDFNKICPMRPQNFAGGAQPPPIHVPPDGQLMIPAFRSF
                     DADFTGLD"
     gene            complement(7800..8267)
                     /locus_tag="VFQ79_20930"
     CDS             complement(7800..8267)
                     /locus_tag="VFQ79_20930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEU0142199.1"
                     /translation="MDTLSQLQRSRLMRSVNTRNTGIEMRVRSVLHAAGFRFSLHKKS
                     LPGTPDILLPRHRVAVFVHGCFWHGHDCSRGKRPSTNVAFWQEKLDRNIERDQQDRNA
                     LKRLGWRVYVVWGCDIEGNTKRLIKLLKHQQAQCSTSIKSRKVSIKRTERRDH"
     gene            8622..9260
                     /locus_tag="VFQ79_20935"
     CDS             8622..9260
                     /locus_tag="VFQ79_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142200.1"
                     /translation="MMRYEDNSGQLGFDALLRQADDSNRQRAFERETAHLPGGMDEAL
                     PFFRSLLEKHDEAMRSADTERVFTLREEARKLALRLNDGKPGILAGENAPACVLERLT
                     AAASGEIPLWGQAGEFVIDAAGTRAHIEMDGIFGIGCSVSFWPGFAARCVDAGKPFIS
                     ETGYRSFLGLHADAIDGMTPDSFTRAVLETYAVRDLKGRLVAVDRKFLSSDA"
     gene            complement(9266..10582)
                     /locus_tag="VFQ79_20940"
     CDS             complement(9266..10582)
                     /locus_tag="VFQ79_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142201.1"
                     /translation="MILKASQRGGGRQLALHLLNAKENEHVHVHELRGFTADDLEGAF
                     KEAYAVSRATKAKQFLFSLSLNPPPNERVSVDAFESAVDAVEKKLGLEGQPRAIVFHE
                     KDGRRHAHAVWSRIDAAHMRAINLPFFKTKLRDVARELFLEHGWQMPRGFIDSRERDP
                     ASYTLAEWQQAKRGGHDPKALKAMLRELWASSDSAKAFAAALRSRGYTLARGDRRGFV
                     AVDYRGEVYAVARYTGQKTKDVRKRLGDDKALPSVDQAKLDHAARMTDMLRRHIRDVE
                     ERRKRETANSFERRRETIQRQRTERDALKKRLEERQAQEARERAARFSKGLRGLWHRM
                     TGKHSKIRKQNEQEALLSHQRDRAERERLIHRQLDERRPLHQQIKRARASHAKQIEEL
                     HRDIADFARTGKEPPKMREQFREAHGPQERVRPRGRDRGREPDFER"
     gene            complement(10592..11044)
                     /gene="mobC"
                     /locus_tag="VFQ79_20945"
     CDS             complement(10592..11044)
                     /gene="mobC"
                     /locus_tag="VFQ79_20945"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEU0142202.1"
                     /translation="MNQAGDFTGSPGLSGSFAASAETRRTKRRRGSPIFPIRFSAEER
                     ARLSKEAGETPLATYIKFRLFNNLPPLETFRPSRLGGRLATDTQLIAKLLAALGDARL
                     ANNLNQLAKAANMGTIDASPDTEKELLAACAEVHAMRRDLIAALGLKS"
BASE COUNT         2322 a         3382 c         3404 g         2328 t
ORIGIN      
        1 ttgtcccgtg tgtcacgaca atggcgtcat caccgggtgg cagaacacgc tttgggacgg
       61 cttcacagat cctgacgcca catcataagg atcgcagcta gggaagacgt cgcaggctta
      121 ccgcttactt tacaacgtcg ccacggacga attcgaaggc atcactggcc taaatctgac
      181 cacgctcgac gacctcgaaa ccctcgcggg ttcgcaaacg aacaaggacg tgttctggga
      241 aaacgtcgtg cgcgtgatcg acaacaccat cggtgttgcc aatctctccg gcggtgatga
      301 aaccgcgctg gatgatgcga tctatgacag cgacaattcg ctctcgctcg ccatcattct
      361 ctccgctctc gaatgggacg gcggcagtgt cacaacatgg aacggcacgt ctggcgacga
      421 cacctacacg ggcggggccg ggatggacac gcttaatgga ggctacggca tcgacacgct
      481 gaacggcagc acgggcgcgg accgcatcaa cggcgaatcc gggaacgaca tactggatgg
      541 ccagagcggc gacgacttcg tgcagggcgg cgctggaaac gacacgtata aatataccgc
      601 cgggcatggc tgggacacgt tcgaggaata tggcacggat acgggcgata aaatcctctt
      661 cgcctcgggc gttgccatcg gcgacctaac catctcgcgc gtatcgagct atgacatgct
      721 gatcgaagtg gcctcgggcg ttggcggcgg gcagattctc atccgcaacc agttcaacac
      781 caaccagcgc attgaaagtc ttgaattcgc gggcggcggc tcgccgtaca acctgctcac
      841 tcagacagcc tggactttga ccgggacgga cggcgacgac cagctctggg gcgtgtgggg
      901 cgcgggctac caggacacga tctatggcgg cgacggcgat gacacgattt atgcctatgc
      961 cgcgaaccac accgacaacg gcgcgaactt cctctatggc gaggacggcg acgacatttt
     1021 agacgcctat tacagctcgg gcgcggacac gctgcgtggc ggcgaaggga acgatacggc
     1081 cactggcggg ggcggtagcg acatctatat ctacgacacc ggccacgaca cctataacga
     1141 agtctccggc actgctgacg aaatccagtt ggctgcgggt atcgtctccg gcgacatcac
     1201 ctatgcgcgc atcagggcct atgacttact catcgaggtc gcgggcgaag gcagcattac
     1261 gatcaaggat ttctactatt cggctggcta ccggatcgag acgctgcgct tctcggacac
     1321 ctcgacggtc aacctggtca acgtcacgcc gacttcgctt aaagggacgc aaggggccga
     1381 cacgattggc ggagccaaca gcttcgatga cgtgatttac ggcttcggtg gcaacgacca
     1441 gctcagcggg aactccggca acgacacgct ctatgggggc gacggcaacg acacactcta
     1501 tggcgggctg gggaacgatt acaacgaagg tggcgctgga aacgacacga tggaagacac
     1561 gtccggcggc aatgacacgt atgtctattc cggcgggctg gattccgtgt acgacaacac
     1621 gggaacggag gtgctgcgcc tgacggggcc gtggacggtg aacgatctgg ccttcagcga
     1681 tctcggcagt catcacaaga tcacgatcac ggccagcacc catgaaatcc agttgctctg
     1741 ggtcgattac agctcggcct acctcatcga gaccatcgag tttgcggatg gcttcacgac
     1801 ctccctgaat acctggaaga cgtggaacaa cggcacgggc tccggcgaga cgctgaacgg
     1861 aacggcgagc gccgacacga tcatcggctt ctccgggaac gatacgctca acgggagcga
     1921 cggaaacgat gccatgcacg gcggcgctga cgacgacacc ttgaacggcg gcaacggggc
     1981 cgatcttctc cacggtggca cgggggcaga cacactcaac ggcaatgacg ggctcgatac
     2041 gctgtacggc ggcgacggcg cggacatttt cctgttcgat gtgagcgcct tcaacaacat
     2101 cgataacgtg aaagacttca atgtcggcca gaacgacgcg ctcgatatcg ccgatatcct
     2161 gacaggctac agcggcgtgg aggatatcaa cgaatgggtc agcttcacga acagcggcgg
     2221 caacacgcaa gtcttcgtgg accgtgacgg cacaggcggc acatacagcg cggcgcagat
     2281 cgccttgctg catggggttg tcgatctcga tgccgatgac ctgctcatga acggcaacct
     2341 gatcgcggtg taagcgagga aagccggtca cagggattga tgcagcagga aaagccgaag
     2401 aagagaaaaa gctttctccg caggaacttt gattttctgc tcatccttct cgtgctcctg
     2461 ccgcttttct atctctacgg gctgttctac ccggtcagcc cgttcgagcc gcgcgaggaa
     2521 tcattcgccc cgtatgagcg gcattatcat ttcagcttcc gcttcccgtt cgttacgtcc
     2581 tacttcctga tggttcccga gaactaccag ccggaaaagc acacctatcc gctggtcctg
     2641 atgctgcacg gcgcgagccg tcatatgtat ggcggcaaag tcctggcgcg gcccatcatg
     2701 cgggagaatc ttccgttctt cgccgttatc ccgatcatgc cgttcggcta tacatgggcc
     2761 tcgccgaaaa cgttctccct gcgcccacaa gccttaccgc tggccatgag cacgatgcgc
     2821 tcggtcatga agaactacag catcaacgag aatcaggtct atgtgacggg ctattcgttg
     2881 ggcgggatcg gcacttacgc agcggtgaca cgctatccgg gtgtgttcgc ggcggcagcg
     2941 ccgaccgatg cttactggaa tcccgagacg gcagaccgga tcgatgacgt acccatctgg
     3001 attttccatg gcgtccagga tcaaggaatg cctgtcgcat atgcccgtat gctggcggca
     3061 gggcttcagc agttgggtcg cgatgtgcgc tacaccgaat acgcaaaccg gggccacggc
     3121 gcatggatac cggcatacga gaatcccgat ttctggatgt ggctcatccg gcagcagaag
     3181 cactagccgg agaagcatct gaaatgtcgc ggtccgggat gggccggagc agaaccggat
     3241 catgacccgg tgatagtacc cgcgctcagg ctggtagttc agcgccaaag gccgaaggcc
     3301 cgtgagccgc aagatgtgtg acgtgtatat acacggcgct cgcgcgccga cacgtcacaa
     3361 tcttgccaag ggtgcgtgcc gccccctgtg gaaccccctg cgcaacgtgt ggccgttgca
     3421 tcaccagtca ggggcgcgtt cctgctcccc cgacaccccc atgaccaagg gagacttccg
     3481 ctctcccctg gaccccatcg aacagggcgg agctttcgcg ctccccctgt acccccatcg
     3541 accaacctcg tcgcggttgg attgcgaccg gggagacgct tcgcttcccc ggaacccctt
     3601 cgagcatggg ctttcgcgcc cctgcaccca cgaccaaccc tgcgcgggtt ggatgccgcc
     3661 aacgttttcg ccgttggatc gcgaccgggg cacacccgaa gcccgtgctt gaagcggcgg
     3721 gccttcaagc ccagccatct tgccaccttc ttgtgaccat cccccggtgg tggcaagcgg
     3781 gttgcattca cccattaata agtagtaaat ggttggttgg atggacgaaa atgaccgcaa
     3841 gaaagaagac gcccggaagg cttaagagga agcaccgaac gaccgccatt gacctgttca
     3901 gcggctgtgg gggcttgtcc ctggggctaa agtcggcagg ttttgacgtt atcgctgcga
     3961 tagacaacga tccgctggcc tgtgcgacgt accggatgaa ccacaaggac acggtactca
     4021 tagagaagga catctcgaag accaagccga cgtacctgag aaagcgtctc ggcctgcgcc
     4081 ccggacagct cgacctgttg gcgggatgcc ccccttgtca gggcttctca actttacgga
     4141 cgctcaacgg caagaagaat gtcaaggagc cgatgaacga tttggtattc cagttcatga
     4201 agtatgtccg cgcctttcgc ccgaaggcga tcatgatgga aaacgttccg ggccttgcga
     4261 aggactctcg ccttgaggag ctgatccgag acttgaaccg tctggggtat cgctgcgaag
     4321 cgcgagtttt gaacgccgct gattttggag ttccccaaag acgcgcgcgg atgattctga
     4381 ttgccatgaa gcggcgcacg ccggtctttg cccagcaagc ccgcgtatct cggagcgttc
     4441 gtagtgcaat tggagcgctg ccgcgcgcgg gcacaggctc tgatccgcta catgattacg
     4501 aagtcaatcg cgccgaacac gttgtcgaac tgatcaagcg tattcccacc aacggcggca
     4561 gccgcaggga cctgcctgaa agcgatcagc tcgactgtca caagaaatgc gatggctttg
     4621 cagacatcta cggtcgaatg gcatgggaaa agccggcccc tacaataaca gggggctgca
     4681 tcaatccctc gaaaggtcga ttcctgcatc ctcgccagca tagagccatc acattgcggg
     4741 aagcggcgct acttcaagga tttccgaaac gttacaagtt tgatctttcg gcggggcgct
     4801 tcaacgcagc gcaaatgatt ggaaatgcgt tcccccccga gtttgccgaa cgtcatgcga
     4861 aggtaattcg aaagcaactg gtggaacagt agcgccagcg agggggtact ttgaccgtag
     4921 ccgaagatgc gctgcgcgca aatctaaacg acgaacaata ccgagcggcg atcgatcctt
     4981 ctgctcaggt gttgacgctg gcgtgtgctg gctccgggaa gtcgagaaca cttgcatatc
     5041 gcatcgcgtg gcttattgca gatagaggcg caacccccga aggaatcgtg gcctttacat
     5101 tcacagagaa ggctgcggac tcaatcaagt tgcgagtatc cgctgcgctt gctgcgtgcg
     5161 aactcgatac aaatcttctc ggtcgcatgt atatcgggac aatccattct tactgtcagt
     5221 atctgctcgg tcgcgctgac gcacggtatc gtcagtttga agtgctggat gacaaccgcc
     5281 tcaagctgta cctgatagag aagtacggcc tgctgcaact gaatcgattt cggccacgtg
     5341 cgaacgatcg ctactttgcc gtgatcggtc aatgccacga cgcatggacg catcacaacg
     5401 aggagatgct cgatccggca accatagaac cccacgaccc tgacctcgcc gcgtactgcc
     5461 gggacttgcg ggagcgcctc attgccgatc agttcctaga tttttccctg atgcaaaggc
     5521 tcgttgtcga tgcgcttcag aatcgcgacc caggcattct ggattcggtt cgacagcttc
     5581 agcacttatt ggtcgatgag tatcaggaca tcaacccgat tcaagaggca ttgatcgggt
     5641 tgctgcatca aaactgccaa acgctgttcg ttgtcggtga cgatgatcaa tccatttacg
     5701 gctggcgagg cgcagacgtt acgaggattc aaacattcac tgatcgtttc ccggcggcga
     5761 gcacgcatac tctggcgcgc aactatcgca gcacaccctt gattgtgagc aacgcagatg
     5821 cgtttgctca tgccgaactt ggcgcaaaca gaatcgtcaa gaacccgcaa gctgacgata
     5881 ccgaccagcc gaatgagttt cgaaatctat ggtttggcac gcgcgccgaa gaagcggact
     5941 gggtcgcaaa caaaatcgcc tcgcttctgg gtacggcata tcgcgaacct aacgggacag
     6001 tccgaggatt aacgcccgca gacttcgcga ttttgatgag gtcaactcga actgcggaaa
     6061 caggtggcaa tcctcctcgc cacgttcctt ttacaagcag gctttcagat cgcggcattc
     6121 tcttcagttt ggaggcgggc ggcgggctct tcgaccgtcc acaggtgcag ctactgaggg
     6181 ctgcgtttga attgctgcgc aatggtcagc cagaccgcaa catcgttcgg cagtttttcg
     6241 atcagcaggt cacgccgttg ttcccgcgaa cggacttcaa tgatctcacg gggttgtttg
     6301 ccacttgggg ccgcgacatt cacgcgccga ccgaccgcgg agtgccgcga cgccggattt
     6361 atccgcagaa gctggttttc gacctgttga atacctttga tgtcgcgaat acccctctcg
     6421 acgatgcgat tcttcaagac atcggcgtat tcagtcggat cattcaggac gtggaagctg
     6481 tctatccgag cgtagacagc acgatgcgat tcacctcgat tctcaacttc ctcaatgttg
     6541 ttgctgagga cggttacgac actagtagct cggatattgt tcagaggccc gatgctgtta
     6601 ccgtttcgac cgttcacaag atgaaggggc tggaatttcc tgtcgtcttt gtagtggacg
     6661 ttgagaacca gcggtttccc gggaaacgac ggaattacgg agggtggctc cccgccgcca
     6721 cgatccaaga ctctttgaat cggggcgcat atcagagcag gcgtgaggac gaggctcgac
     6781 tgttttatac ggcgctcacg cgcgccgagc ggttcctgta tgtcaccggc agcgcaaacg
     6841 cgccgggctg ggggagacca aagcgaccgt caccgtttgc ggcacatcta gctgacgcac
     6901 gtattgtgac tgatgcgacg gcggcaaccg ccgggcttgt caacgctcca cccgcccgcc
     6961 gcatcgaagc cagcaacttg cctaccagct attccgacat ccgctactat ctgcgttgcc
     7021 cgaaggacta ccactaccgg agaattttcg ggttcagccc gagcattcct gatctattcg
     7081 gattcggaat gacagtacat gcgtccatcg gaaaacttca tcaacagttt ccagctcaac
     7141 gtccaacagc agcccaagct gaggccgttg tgcaggacat ttttcacctg aagcatgtcc
     7201 aacagagcaa tgatccggtg gggcgacccg gtccctacga acgtgctcgc actcgcgctc
     7261 aggaactggt gtcgggatat gcgcagcaat tctcaacaga tttcgaacgc cggcgcgagg
     7321 tcgaggcgcg tttcgagatt cctgtaaatg gcgcggttgt atccggtgcg attgatctca
     7381 tgcttgaaga agacgagaac gggaacgtcg tcgatgcctg tgttgtggac ttcaagacgc
     7441 tcggaggggg cgacgatcca ttggagaatg cagacctcga atggactgag ctagcgttgc
     7501 aggtacagct ctacgcgaaa gccgcccgtg acgtattggg gcatgttgcc gatgaaggct
     7561 ttgtccactt gctgaaggac ggtcagcggc tgcaagtgcc cgtgggcgat gcggctgtgg
     7621 gcgcggcgtt agccaatgtc gagtgggccg tggaccgaat cgttgcatcg gattttccga
     7681 tgcgccccca ccgtacaaag tgcgaagact gcgacttcaa taaaatctgc cccatgcgtc
     7741 cccagaattt cgctggcggc gcgcagcctc cgcccatcca tgtaccgccc gatggtcagc
     7801 taatgatccc tgcgttccgt tcgtttgatg ctgactttac gggacttgat tgatgtactg
     7861 cattgagcct gttgatgctt caaaagctta attaatcgct tggtgtttcc ctcgatgtcg
     7921 cagccccaca ccacgtacac gcgccatccc aaacgcttca acgcatttcg atcttgttga
     7981 tcccgttcga tattgcggtc caatttttct tgccaaaacg ccacgttcgt tgaaggccgt
     8041 tttcctcggc tgcaatcgtg gccatgccag aagcaaccgt gcacgaatac tgcgacacga
     8101 tgccttggca gaaggatgtc gggcgttccc ggaagagatt tcttgtgaag cgagaagcgg
     8161 aaacctgctg cgtgcagcac ggagcgaacg cgcatttcaa tcccggtgtt tctcgtgttc
     8221 accgagcgca taagcctgct gcgctgtagt tgtgaaagcg tgtccactat gactcccatc
     8281 gaggggctgt gcgcggctgc ttgccgtgta gcaggctaga ttcaagagtg caagctgcta
     8341 ggcacgcaac ttccaattgg tagattgggg cgcctcgcta cttcgccgcg accgggctct
     8401 atctccgctg cgctccgacc aagccctacg gtcttggccc atccgggtga cgatccctgg
     8461 cgcaagagaa gaaggcccct tcggcccatc cgtcaaggca accccttgcg gggcggaaat
     8521 cgaagacgat ttccggcctt gaccgatttc ctcccgctcg tgaagacggt tgtccatggg
     8581 gttgcatagg caacccctct ttccaaggaa aggacttgaa gatgatgaga tacgaggaca
     8641 attccggcca gcttggcttc gatgctttgc tgaggcaagc cgatgacagc aaccgccaac
     8701 gcgccttcga gcgcgagacc gcgcacctgc cgggagggat ggacgaggca ttgcccttct
     8761 ttcgctcgct tctcgagaag cacgacgaag cgatgcgctc cgccgacacg gagcgcgtgt
     8821 tcacgcttcg cgaagaggcg cgaaagctgg ccctgcgcct gaatgacggc aagccgggaa
     8881 tactcgcggg cgaaaacgct ccggcctgcg tgctggagcg attgaccgcc gccgcttccg
     8941 gagaaatccc gctctggggg caggcaggcg agttcgtgat cgacgcggct ggcactcgcg
     9001 cccacatcga aatggacggc attttcggaa tcggttgctc ggtttccttc tggccgggct
     9061 tcgccgcgcg ttgcgtggac gccgggaagc cgtttatcag cgagacgggc taccgcagtt
     9121 ttctcggcct tcatgccgat gccatcgacg gaatgacacc ggatagcttc acccgtgccg
     9181 tgctggagac ctatgccgtc cgcgatctca agggcaggct tgtagcggta gacaggaagt
     9241 ttctttcgtc agacgcctga gccggtcagc gctcgaaatc cggctcgcgg cctcgatccc
     9301 ttccgcgcgg acgcacgcgc tcttgggggc cgtgcgcctc gcggaattgc tcccgcatct
     9361 tcggcggctc cttgcctgtt cgcgcgaagt cggcgatgtc gcggtgaagc tcttcgatct
     9421 gtttggcgtg cgatgctcgc gcccgcttga tctgctggtg aagcgggcgg cgctcatcta
     9481 actggcggtg aatcagccgt tcacgttcgg cgcggtcgcg ctgatgcgat aacagcgctt
     9541 cctgttcgtt ttgctttctg atctttgaat gcttgcctgt catcctgtgc cacaggccgc
     9601 gcaagccctt cgagaaacgc gccgcgcgtt ctctcgcttc ctgcgcctgc cgctcttcga
     9661 gtcttttctt cagcgcgtca cgctcggtcc gctggcgctg gatggtttcc ctccggcgct
     9721 caaagctgtt ggcggtttcg cgcttccggc gctcttccac gtccctgatg tgccgccgga
     9781 gcatgtcggt catgcgggcg gcgtgatcta acttggcctg atcgacggaa ggaagcgcct
     9841 tgtcatcgcc gaggcgcttc cttacgtcct tggtcttttg tccggtgtac ctggctaccg
     9901 cgtaaacctc gccccggtaa tcgacggcaa cgaagccgcg ccggtcgccg cgagcgagcg
     9961 tgtagccgcg agagcgaagc gcggcggcga atgctttggc ggaatccgat gaagcccaca
    10021 gctcccggag catggctttg agcgctttcg ggtcatgccc gccgcgcttg gcctgctgcc
    10081 attcggcgag cgtgtagctg gccgggtcgc gctcgcggct atcgatgaag ccgcgcggca
    10141 tctgccagcc atgttcaagg aacagctcgc gggcgacatc ccggagcttc gtcttgaaga
    10201 acgggagatt gatcgcgcgc atgtgggcgg catcgatccg cgaccagacg gcatgggcgt
    10261 gcctgcgccc gtccttttca tggaagacga tggcgcgagg ctggccttcg agcccgagct
    10321 tcttttcgac ggcatcgacg gcggactcga acgcatccac cgatacccgt tcgttcggcg
    10381 gagggttcag ggacaacgag aagaggaatt gcttggcctt cgtggcgcgg ctgaccgcat
    10441 aggcttcctt gaacgcgccc tcaaggtcgt cagccgtgaa gccccgcagc tcgtgaacat
    10501 ggacgtgctc gttttccttg gcgttcagca ggtgaagggc gagctggcga ccgccgccgc
    10561 gctggctggc tttcaggatc atggccggac ctcacgactt caggccgagg gccgcgatca
    10621 gatcgcgccg catggcatgg acttccgcgc aggcggccag cagctccttt tccgtatcgg
    10681 gggatgcgtc gatagtcccc atgttggcgg ctttggcgag ctggttgagg ttgtttgcca
    10741 gccgggcgtc acccaaagcg gccagaagtt tggcaatgag ctgggtatcc gtggccagac
    10801 ggccacccag acgggagggc cggaaggtct ccaggggcgg caggttattg aacagccgga
    10861 acttgatgta ggtggccagc ggggtttccc cggcctcctt gctcagccgt gcccgttcct
    10921 ccgccgagaa ccggataggg aaaatcggcg agcctctgcg ccgcttcgtg cgccgggttt
    10981 cggcggacgc ggcaaagctt cccgaaaggc ccggactgcc cgtaaaatcg ccagcctggt
    11041 tcatagtgac ggctccgggg ccggagctgc caggcgctca agctcttgat tccattcttc
    11101 gagagtctgg aagagcgcgt caggatcaat cgcatccggg ttcgaacttt ctctgcccgg
    11161 gtgcgccaat tcctgtcgac ccagggttcc ggtgggttgg tcggatctcg gtcggatctc
    11221 tgcggtcgga tctctggaca cccaccacta ggctcggatc tctggtaacc ggatctctgg
    11281 ccggatctct ggacaccacc actaggctcg tggtagggat ctctggacac ccaccactat
    11341 gccaatgggt cggatctctg gacacccatc ggctgggcgg atctctggac acccaccact
    11401 aggctcggat ctctgggggg atctctcata gggatc
//
LOCUS       DASTMK010000507         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_378003, whole genome shotgun sequence.
ACCESSION   DASTMK010000507 DASTMK010000000
VERSION     DASTMK010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378003"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            608..979
                     /locus_tag="VFQ79_20950"
     CDS             608..979
                     /locus_tag="VFQ79_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0142203.1"
                     /translation="MHKKKVLAAVEDLFFTVKINDAAKRAGLDVEFLKSEKDVLDRAK
                     ANPTLIIVDLNNAAIQPLKLITKLKGNSDLKQISVISYLSHVQGELKQKAHEAGADMV
                     LARSAFSQNLPQILKRHAGVR"
     gene            1069..1500
                     /locus_tag="VFQ79_20955"
     CDS             1069..1500
                     /locus_tag="VFQ79_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142204.1"
                     /translation="MSEYALIDPRSVYRLPANFDFQDYIGTERELWALFPETDGRRVN
                     FFQIGITARLFVEVRDDGSNLAENETYVFMPCDDNTMRPIRMEAGRRLTLTEYRMAHL
                     TAIRMLGKLNPAGTVMQKIGRAILGQELLEEVRTRIQDTTE"
     gene            complement(1507..2538)
                     /gene="trpD"
                     /locus_tag="VFQ79_20960"
     CDS             complement(1507..2538)
                     /gene="trpD"
                     /locus_tag="VFQ79_20960"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU0142205.1"
                     /translation="MTLLPYLHRICAGENLPSQDAEQAMALVLSGEVSTPQIAAFLVA
                     LRMKGETAGEVLGFARAMRAKASRVNAGTSNGDVLLDTCGTGGDGTGTFNISTIAAFV
                     VAATGVWVAKHGNRAISSQCGSADILEALGVNIHLNTAQMGEALRKVGISFLFAPAIH
                     PAMKHAQAARTELKMRTVFNLLGPLTNPAGATVQLVGSHSFRSAELMAEALANLGIRR
                     GFVVHGADGLDEITTTGETIALEIQGGAIAHHTLTPDDFGLRPATREALQGGDRVVNC
                     QIAEAVLEGSLGPHRDIVLANASAALVAAGKAPGFREGVSLAAEAIDSGAAKRKAREL
                     AEFSRAMTP"
     gene            2590..>3078
                     /locus_tag="VFQ79_20965"
     CDS             2590..>3078
                     /locus_tag="VFQ79_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU0142206.1"
                     /translation="MKIHEYQAKGLLAKYGVPVPRGEVAASAEEAGQAAARIGGSVVV
                     KAQIHAGGRGKGGGVKVAKDAAEAREIAGKILGMTLITHQTGPEGRVVKRVLVEETLP
                     ITSELYIGVVLDRTQAKPVMMASSAGGMDIEEVAAKTPDKILKEIIEPGLGLTPFQAR
                     KLA"
BASE COUNT          683 a          892 c          895 g          608 t
ORIGIN      
        1 ccaaggtggt agctttcgtc tggcgatctc cccccggacg ggcggaaacg atggaaccct
       61 ggtgataaat ctggggattt acgggaaaag aaagttgaga caacgtttca agtaagtttt
      121 ctaaaagacg aggctcaact gagatggtga tggaaatcag tcttccactg gtgccggaca
      181 ggacggcagt tgatggaccg gggctcggcc acgcgctcat gtatcctcct cgcgccccga
      241 gggggcgccg catcagcgcg ccggccgcac aaataaagaa ccctgaagct aaaggctcca
      301 gggttagata ttttcggaag accgacccgc ctttagcact tttttttacg tggttgcaag
      361 tagcctggac cgttgttcag atcctaaatc aatccacgtc aacctcagca taacaccgcg
      421 atacggcaga atcaaccaaa ttctgcatgt agaaaacccg cttgctcacg cgcgcggctc
      481 agaatcggag cggttggctg cgcttgcggc gcggcggagg gcggattaca ctggaggtag
      541 ccttccttgt cctcacgatt ccgtattgga aaaaggtaag cgatttctag agatacgttc
      601 caactctatg cataagaaga aagtactggc agcggtcgag gacctcttct tcacggtaaa
      661 gatcaacgac gccgccaaac gagcgggttt ggatgtcgag ttcctgaaat ctgaaaagga
      721 cgtgctggac cgggccaagg ccaatccgac actgatcatc gtcgatttga ataacgccgc
      781 tatccaaccc ttgaagctga tcacaaagct gaaaggcaat tcggatttga agcagatcag
      841 cgttatcagc tatctttcgc acgtgcaggg cgagttgaag cagaaggctc acgaagccgg
      901 cgcggacatg gttcttgccc gctccgcgtt ttcccagaac ctccctcaaa ttctcaaacg
      961 ccacgccgga gtacgatagt tcagtttttc ggagcagatt cccggctgga atatacttct
     1021 tcagttagaa gtggctttca gccgaattta aggtaagagg cgcaatacgt gtcggaatac
     1081 gctttaatcg atccgcggtc cgtttaccgg ctccccgcaa attttgactt ccaggactac
     1141 atcgggaccg aacgagagct ttgggcgctt tttccggaga cggacggaag gagagttaac
     1201 ttctttcaga tcggcatcac cgcgcggctg ttcgtggaag tccgggatga tgggtcgaat
     1261 cttgcggaaa acgagactta cgtcttcatg ccttgtgacg acaatacgat gcgtcccata
     1321 cgcatggagg ccggccggcg gctcacgctc accgaatatc gcatggcgca cctcactgcg
     1381 atccgtatgc tcggcaagct caatcccgca ggcacggtga tgcagaagat cggacgggcg
     1441 attctcgggc aggaactcct ggaggaagtc cgcacgcgaa tacaggatac aaccgagtag
     1501 cgtcccttag ggtgtcattg cccggctgaa ttccgccagt tccctggcct tccgttttgc
     1561 ggcgccggaa tcgatggctt cggccgccag cgacacaccc tcacggaaac cgggcgcctt
     1621 gccagcagcg accagtgcgg cggaggcgtt tgcgagaaca atatcccgat gcggtcccag
     1681 ggaaccttcg agcaccgctt ccgcgatctg acagttcacc acacgatccc caccctgaag
     1741 agcctccctg gtcgcgggcc tcaatccgaa atcgtcgggc gtcaatgtgt ggtgcgcaat
     1801 cgcaccgccc tggatttcga gcgcaatcgt ctcccccgta gtggtgatct catccaatcc
     1861 atcggcgccg tgaaccacga agcccctgcg gatccccaga ttcgccagcg cctcggccat
     1921 cagttcggcg gagcgaaacg aatgggaacc cacaagctgc accgtcgctc ccgcgggatt
     1981 ggtcagcgga cccaggaggt tgaataccgt ccgcatcttc aactccgtcc gggccgcctg
     2041 cgcgtgcttc atcgccgggt gaatcgcggg tgcaaacaga aacgaaatgc cgactttccg
     2101 gagcgcttca cccatctgtg cggtattgag atgaatgttc acgcccaggg cttccagaat
     2161 gtcggcgctc ccgcattggc tcgaaatcgc gcggttccca tgcttggcca cccatacacc
     2221 ggtagccgcc accacaaacg ccgcgatggt ggagatgttg aaagttcccg tcccgtctcc
     2281 gccggtcccg caggtatcga gcagcacgtc gccgttgctg gtgcccgcgt ttactcgcga
     2341 ggctttggct cgcattgctc gtgcaaagcc caacacttcg ccggcggttt cgcctttcat
     2401 gcgcaaggct accagaaaag ccgcaatctg aggtgtactg acctccccgc tcaggaccag
     2461 cgccatcgct tgctcggcgt cctgtgacgg cagattctca ccggcacaaa tccggtgaag
     2521 atagggcagc aacgtcatgt tatgattgaa ctttcggctt tctagcaata ctaacacaga
     2581 cgcaatccta tgaaaatcca cgagtatcag gccaagggcc tcttggccaa atacggcgtc
     2641 cctgtgccac ggggagaggt cgccgcctcc gctgaagagg ccgggcaggc tgccgcgcga
     2701 attgggggga gtgtcgtcgt aaaggcgcaa atccacgcgg gcggccgggg aaaagggggc
     2761 ggcgtgaagg tggcgaagga tgccgccgaa gcgcgcgaga tcgccggcaa gatcctgggt
     2821 atgacgctga ttacgcacca gacgggtccg gagggacgcg tcgtcaagcg ggtgctggtc
     2881 gaggaaacgc tgccgatcac aagcgagttg tatatcggcg tcgtcctgga ccgcacccaa
     2941 gcaaagccgg tgatgatggc ctcctccgcg gggggcatgg acatcgaaga ggtggccgcc
     3001 aaaactcccg acaagatttt gaaagagatc attgagccgg ggctggggtt gacgccgttt
     3061 caggcacgga aactcgcg
//
LOCUS       DASTMK010000508         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_410012, whole genome shotgun sequence.
ACCESSION   DASTMK010000508 DASTMK010000000
VERSION     DASTMK010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410012"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="VFQ79_20970"
     CDS             complement(<1..1573)
                     /locus_tag="VFQ79_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU0142207.1"
                     /translation="MHTNRLANEKSPYLQQHAHNPVDWYPWGEEAFERARREDKPIFL
                     SIGYSTCHWCHVMERESFESESLAEVLNRFFIPIKVDREERPDVDRVYMTFVQATTGG
                     GGWPMSVWLTPELKPFLGGTYFPPDDRYGRPGFRSVLEQIAKAWESDQTRILQSSHDV
                     LAELEKHVQVKGDAAYLDDRILESGFNVFRRTYDVRLGGFGNAPKFPRPAVFNFLLRY
                     YRRTGNQEALHMTVNTLKAMARGGMYDHLGGGFHRYSVDARWFVPHFEKMLYDQAQLA
                     IAYLEAFQITGEAWLQETARGILDYVLRDMRSPEGGFYSAEDADSVIDPAQPHEKGEG
                     AFYIWSAGEIRSLVDSPVLDWFQYRYGVEAAGNVQEDPQGEFTGRNILYEAHTVEETA
                     ERFDLPEAEIRRGLEEATQKLLAHRSGRIRPHLDDKILTCWNGLMISAFAKAGQVLGE
                     SKYSEAASTAADFILSKLYERYSGRLLRRFREGEAAIPGFLDDYAFLAQGLLDLYETT
                     FDIHHLDTAIRLTETM"
     gene            1691..3046
                     /locus_tag="VFQ79_20975"
     CDS             1691..3046
                     /locus_tag="VFQ79_20975"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU0142208.1"
                     /translation="MTYYEQHADKFLEGLKSFLRIPSISTASEHKDDIRKAAGFVAGE
                     LRSAGLDHVELIEGEGNPLVYGEWMGAPGKPTVLFYGHYDVQPPEPLEEWQSPPFEPD
                     IRNDSIYARGASDDKGQTYIQIKAVESLLKTTGRLPVNVKFLIEGEEEVGGEHISNYV
                     ASKPSRLQADAAVICDTEMFAPELPTICVGLRGIVYGELVVQGADHDLHSGVYGGAAP
                     NAIQATAEILCTLKDREGHILIPNFYDRVRPPSEKEREAWARLPFNEDEYRTKEMGAS
                     ALVGEPEVPLFERLWARPTLEVHGIRGGFTGEGAKTVIPARAVAKVSMRLVADQDPEE
                     AARQLQSAVVSICPRGVKAEFRAIHSAGPSLVDPQNPFIEAAAAAMTGIFGNETVYIR
                     SGGSIPIVGLFDQHLGIPSVMMGFGLPDDNLHAPNEKLYLPNFFRGIQAVAKYLEILG
                     Q"
     gene            3043..>4168
                     /locus_tag="VFQ79_20980"
     CDS             3043..>4168
                     /locus_tag="VFQ79_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142209.1"
                     /translation="MTLCQNKNIPSGGSMLTFKTIRRSGLLLLFYASIWGADATLPVP
                     SNLKVQGIPSIPTALADRLAQYGEFRTANLLDWHPTKREILISTRFADVPQVHRVLMP
                     GGAREQLTFFPERVTGALYQPGSAASFLYAKDIGGSEFYQLFLFDLQSGASRMLTDGK
                     SRNTSPQWSHDGSRIAFASSQRNGRDMDIYVIDAKRSGPAKLLLQVQGGGWYPTGWSP
                     DNSRIVLEHYTSINESAIHLLDPNTGAALPLTPSDQVAAYANAALTPDGKGAYLITDR
                     DSEFRRLAYIDLATKQFTFLRPELKWDVAALRLSRDGRKLAYVVNEDGADTLHVIDTA
                     TRRDLSLPKLAYGSFTSLSWHNNSLEVGFSLASAKSPSDIY"
BASE COUNT          855 a         1233 c         1239 g          841 t
ORIGIN      
        1 acatggtttc ggtaagccgg atggcggtgt cgaggtgatg aatgtcgaac gtggtttcgt
       61 acaggtcaag cagtccctgc gccagaaacg catagtcgtc gaggaatcct gggatggcgg
      121 cctcgccctc acgaaagcgg cgcagaagcc ggcccgaata gcgctcatac agtttcgaca
      181 ggatgaaatc cgccgccgta gaggcagctt cgctgtattt tgattcgccg agcacctgcc
      241 cggcttttgc gaaggcagaa atcatgagcc cgttccagca ggtaagaatc ttgtcgtcga
      301 ggtgcgggcg gattcgcccg gagcgatgcg caagcagctt ttgcgttgcc tcttcgagac
      361 cgcgccggat ctccgcctcg ggaagatcga agcgctccgc cgtctcttcc acagtgtgtg
      421 cctcgtaaag gatgttcctg cccgtaaact ctccctgcgg atcttcctgc acgttgccgg
      481 cagcctccac gccatagcgg tattgaaacc agtcaagcac cgggctatcg acgaggctcc
      541 ggatttcccc ggcactccag atatagaacg cgccctcgcc tttttcgtgc ggctgggctg
      601 gatcgatcac gctgtcggca tcctcggcgg aatagaatcc tccctcgggg ctcctcatat
      661 cgcgaaggac gtaatcgaga atgccgcgcg cggtctcctg aagccaggct tctccggtaa
      721 tctggaacgc ttccagatat gcgatcgcca gttgtgcctg gtcatatagc atcttctcga
      781 agtgtggaac gaaccagcgc gcgtccacgg agtaccggtg gaagcccccg ccgagatggt
      841 cgtacatacc accgcgcgcc atggccttca aggtattcac cgtcatatgg agcgcttcct
      901 gattgcccgt ccgccggtaa tagcgcagca gaaagttgaa gacggccggc cgcggaaact
      961 tcggtgcgtt gccgaaaccg ccgagccgca cgtcgtacgt acggcgaaag acattgaagc
     1021 ccgattcgag aatccggtcg tccaaatacg cggcatcgcc tttgacctga acgtgctttt
     1081 ccagttccgc gagcacatca tggctcgatt gcaggattct ggtctggtcg gattcccagg
     1141 cttttgcaat ctgctcgagc acggagcgga atcccggccg tccgtagcga tcgtccggag
     1201 gaaaatatgt gccgccaagg aacggcttca gctcgggggt gagccatacc gacatgggcc
     1261 agccgccgcc tcccgtcgtc gcttgaacga aggtcatata gacgcgatcg acatccggac
     1321 gctcttcccg gtccactttg atcgggatga aaaaccggtt cagcacctcc gccaacgatt
     1381 cggactcgaa tgattcccgt tccataacat ggcaccagtg gcaggtagag tagccaatcg
     1441 acaggaagat gggcttgtct tcccggcgcg cgcgctcgaa tgcctcttcg ccccagggat
     1501 accagtccac cgggttgtgc gcgtgctgct gaagataagg actcttttcg tttgctaagc
     1561 gattggtatg catgtgataa cctccggcgt tcccaggccg gcgaggaggg taacctttat
     1621 tctagtctac agaccggttg acccggcacc gcgcggcaat tcatcctggt tgtgccgcga
     1681 gacaattccc atgacctact atgaacaaca cgctgacaaa tttctcgaag ggctcaagag
     1741 ctttctccgc attccgagca taagcactgc ctccgagcac aaagacgaca tccggaaagc
     1801 cgccgggttt gtcgccgggg aactgcgctc ggccggtctc gaccacgtgg agctgatcga
     1861 aggcgaaggc aatccccttg tttacggcga gtggatgggg gcccccggca aacccactgt
     1921 actgttttat gggcactatg acgtgcagcc gccggaaccg ctggaggaat ggcagtcgcc
     1981 gcccttcgag ccggacattc gaaatgacag catctatgcg cgtggcgctt ccgacgacaa
     2041 gggtcagacg tacattcaga tcaaggccgt cgaatcgcta ctgaagacga ccggcaggct
     2101 gcctgtaaat gtgaagtttc tcatcgaggg agaagaggag gtcggcggcg agcacatatc
     2161 caactacgtt gcctccaaac cgtcacggct gcaggcggac gcagccgtga tttgcgatac
     2221 ggaaatgttc gcgccggaac tgccaaccat ctgcgtcgga ctacggggca tcgtctacgg
     2281 tgaattggtt gtccaggggg ccgatcatga tttgcattcg ggcgtatatg gcggtgccgc
     2341 gcccaacgcc attcaggcga cggccgagat tctctgcaca ctcaaggatc gcgaagggca
     2401 cattctgatt ccaaacttct acgatcgggt gcggccgcct tccgaaaaag agcgagaggc
     2461 ctgggcgcgg ctgccgttca atgaggacga gtaccggaca aaagaaatgg gagccagcgc
     2521 gctggttggc gaacccgagg ttccgctgtt tgagcggctg tgggcgcggc ccacactcga
     2581 agttcacgga atccgcggcg gattcacggg agagggagcg aagaccgtca tccccgcccg
     2641 cgccgtcgcg aaagtgagca tgcgcctcgt agcggaccag gaccccgaag aggcggccag
     2701 gcagttgcag agcgcggtcg tcagcatctg tccgcgagga gtgaaggccg aatttcgcgc
     2761 gattcacagc gcgggaccct ctctcgtaga ccctcagaat cccttcatcg aagccgcggc
     2821 agcggcgatg accggcatct tcgggaacga gaccgtctac atccgctcgg gcgggtccat
     2881 tcctatcgtc ggtctgttcg atcagcacct gggaattccc agcgtcatga tggggttcgg
     2941 actgccggac gacaacctgc atgcgccgaa cgagaagctc tacctgccga acttcttccg
     3001 cggaattcaa gccgtggcga agtatctgga gattctcggc caatgacgct ctgccaaaat
     3061 aagaacattc catcgggagg ttcgatgctg acattcaaga cgatccgccg ttcggggctg
     3121 ctgctcctgt tttacgccag tatttgggga gcggacgcaa ccttgcctgt accgtcgaat
     3181 ctgaaggtgc agggaattcc ttccattcca acggcactgg cggaccgtct ggcgcaatac
     3241 ggcgagttcc ggacggccaa tttactggat tggcatccaa ccaaacggga aatcctgatt
     3301 tcgacgcgct ttgcggacgt gccccaggta caccgggttt taatgccggg tggtgcgcgc
     3361 gagcaactga cgttcttccc ggaacgggtg accggtgcgc tgtatcagcc cggcagcgcc
     3421 gcgtcgtttc tgtacgctaa ggatatcggc ggatcggaat tctatcagtt gttcctgttc
     3481 gacctgcaat cgggcgcatc cagaatgctg acggatggaa aatcccgcaa cacgtctccg
     3541 caatggtctc acgatggctc ccgaatcgcg tttgcgtcga gccagcggaa tggccgcgac
     3601 atggatattt acgtgattga tgcgaagcgg tccggaccgg cgaagctact gcttcaggtg
     3661 cagggcggcg gctggtatcc cacgggctgg tctcccgata acagcaggat cgtgttggaa
     3721 cactatacat cgatcaacga gagcgccatt cacctgttgg accccaatac gggcgctgct
     3781 ttgccgctaa cgccctcgga ccaggttgcg gcatacgcaa atgccgccct tactccggat
     3841 gggaaaggcg cctacctcat cacggaccgc gactcggagt ttcgccggtt ggcctacatc
     3901 gacctggcca cgaagcagtt tacatttctg cgtccggagt tgaagtggga cgtcgctgcg
     3961 ctgcgcctgt cgcgcgacgg gcgcaagctg gcttacgtag tgaacgaaga cggcgcggac
     4021 acgttgcacg tcatagacac ggcgacgcgc cgggacttat ccttgccgaa actggcgtac
     4081 gggtcgttta cttcgctgtc ctggcacaac aacagcctcg aggtcggctt ttccctcgct
     4141 tcggccaaaa gtccttcgga tatctatt
//
LOCUS       DASTMK010000509        13383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_153355, whole genome shotgun sequence.
ACCESSION   DASTMK010000509 DASTMK010000000
VERSION     DASTMK010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13383
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153355"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFQ79_20985"
     CDS             complement(<1..377)
                     /locus_tag="VFQ79_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142210.1"
                     /translation="MDRCGYSMVFVTPVNPARHPLLRKYPNAPHPFVLVPPAASATLL
                     SPGTAMTARVTLIGNGAVFLPHFIRVFEAMGASGRYGGAFHLEDIVSPLDPGCILYDP
                     GSRILRRPPVWSPPETSAPVSRI"
     gene            979..1176
                     /locus_tag="VFQ79_20990"
     CDS             979..1176
                     /locus_tag="VFQ79_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142211.1"
                     /translation="MAIKERLELSARILASIDGRRNHKGDHSIFSSVERQVIDRELKE
                     LAEEWALNPDSPAVPGIWRLR"
     gene            complement(2097..4700)
                     /locus_tag="VFQ79_20995"
     CDS             complement(2097..4700)
                     /locus_tag="VFQ79_20995"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0142212.1"
                     /translation="MNVFELRERLINDYSSYIRSFIRIRDERIEGLVSSYLDQGLLWP
                     DPLIQLNPSFQYAGTIDDLVGEELLHAECSRVFRAAKEKGDGKPLRLYQHQADAIRRA
                     RAGRNYVLTTGTGSGKSLAYIIPIVDHVLRNGTGRGIQAIVVYPMNALANSQFGELGK
                     FLKLGYPDGKGPVTFARYTGQEGDEERNAIVANPPDILLTNYVMLELILTRVYEKQLV
                     KAAQGLRFLVLDELHTYRGRQGADVALLVRRAREFFGARKLQVVGTSATLAGPGTYDQ
                     QQAEVAAVATVLFGAEVAPSDVVGETLQRATPEENIADPVYLNSLRERVENDGSRSPL
                     TYQAFTADPLSSWIEGTFGVYRDNASDRLRRRRPRTITGQHGAAGELSRVTGADEMKA
                     KAVIQEALLAGYTCERNPETGFPAFAFRLHQFISRGDTVYASLESESNRYLTFNRQQF
                     VPGDRRKILLPMVFCRECGQEYYCVRSLEDSDSGAVTYIPRELSDRSGGDGSKAGFLH
                     FSAERSWPSGTDEELAKLPEDWVEDTNQGPRVKASRKKNLPRPVTVSPDGREMVDGIS
                     CHFVESPFRFCLHCRVSYDFRTTSDFGKLTSLGTEGRSTATTILSLATIRHLRKEGSL
                     APRARKLLSFTDNRQDASLQAGHFNDFVEIGLLRSALLKACSAAKPEGLTHEVFTQRV
                     FDALGLPFASYSKDPDLKFQAKLQTERAFRDVIGYRLYHDLRRGWRVTSPNLEQCGLL
                     EIDYLSLDDLSKDVASWQNLHPALAGASPVTRCRIAKTLLDFMRRELAVQVDYLDATK
                     QETIQQQSNQRLIAPWALDENEVLEHAATLYPRSVRQGDSGGDVFVTARSGFGLYPGM
                     AAVHPSRVRPG"
     gene            5666..6760
                     /locus_tag="VFQ79_21000"
     CDS             5666..6760
                     /locus_tag="VFQ79_21000"
                     /inference="COORDINATES: protein motif:HMM:NF037501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HEU0142213.1"
                     /translation="MAESVGERVPGVLLQASRSVCRFLEEVLPTLSASWWADLVVANL
                     SFQQRRAVESRRIAALSGLDLAALLRVLDANWHGIADRLSLPSEARHYLKEMRTVRDR
                     WAHASASEFSPDDVYRDLDTLQRFLVSIGADEELLGEVKRLKGASLAAGGQMSSTPET
                     PAPPLASAFSVGQVVCLRSNPDTTGAVIGIHPGHPEARYSVFQSGTIATYYESQLRAA
                     DVAGGLEVISLQEFDAYLSALQIRHPSTSIVYSLNAARIDCVPYQFRPVLKLIRAERP
                     RLLIADGVGVGKTIEAGLVLKELQARTDIQRVLVICSKPLITERKWESEMKRFDERFI
                     PLDGDKLRYCVNETDLDGVWPDQYAKATRR"
     gene            6757..7023
                     /locus_tag="VFQ79_21005"
     CDS             6757..7023
                     /locus_tag="VFQ79_21005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU0142214.1"
                     /translation="MKPVELVERAVRNSSKSRDTVLDPFGGSGSTLIACEKTGRHARV
                     IELEPKYCDVIIRRFQEFSGKPVTLESDERKFSEVAAERLQVAT"
     gene            7275..7412
                     /locus_tag="VFQ79_21010"
     CDS             7275..7412
                     /locus_tag="VFQ79_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142215.1"
                     /translation="MEHRLFLKINGTYIRRRECELIEQNMNKRVESLAQRVQERETAG
                     D"
     gene            complement(7409..7741)
                     /locus_tag="VFQ79_21015"
     CDS             complement(7409..7741)
                     /locus_tag="VFQ79_21015"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0142216.1"
                     /translation="MTKKKSSRLAKALLETAAGMHRVGVLKDDNFHKITVRHLGEAPL
                     RTSRPITPEEIREIRERAKLSQAAFARYLNLTSGYVSQLERGAKRPSGPALALLNVVR
                     AKGVEVLL"
     gene            complement(7722..8105)
                     /locus_tag="VFQ79_21020"
     CDS             complement(7722..8105)
                     /locus_tag="VFQ79_21020"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0142217.1"
                     /translation="MQVFKTKWLARFAKHEGIADASLHEAIARAERGLVDADLGGGLI
                     KQRVARRGKGRSGGYRMLIAYRLKKRAIFLYGFAKNERANIGADELLTAREIASPWLA
                     ADARQIARALAEGELQEVPYDEEEI"
     gene            complement(8213..8788)
                     /locus_tag="VFQ79_21025"
     CDS             complement(8213..8788)
                     /locus_tag="VFQ79_21025"
                     /inference="COORDINATES: protein motif:HMM:NF023420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3489 domain-containing protein"
                     /protein_id="HEU0142218.1"
                     /translation="MATFTIDTENNITAFAEYEDALNHRIGSTEGTFASEKELAKLTS
                     QWPIGRFIDAWNTFAGVVPFDALKPVKKFTDRKTAVARIWKAVQALTPAPAQHAAPVA
                     TKKAKATKEAKPKGDAAGPKEAREGSKKAIVLDMLRRPEGATLADIQSATGWLAHSVR
                     GFISGGLSKKMGIKVESFKSDGGDRAYRIAQ"
     gene            complement(8804..9037)
                     /locus_tag="VFQ79_21030"
     CDS             complement(8804..9037)
                     /locus_tag="VFQ79_21030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142219.1"
                     /translation="MPMTREELTTWAMQNGWKPDRWGHLKKEFDNGTHRLKLSRIAAR
                     HEIATPFGWARIASGYLKNLQLTADGKLAGMNR"
     gene            complement(9042..9212)
                     /locus_tag="VFQ79_21035"
     CDS             complement(9042..9212)
                     /locus_tag="VFQ79_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142220.1"
                     /translation="MQCTLCEEPRPIHRTLTLTKADGELLDSANYCEDCWNDIRASVE
                     DASGLRDGWRED"
     gene            complement(9228..9476)
                     /locus_tag="VFQ79_21040"
     CDS             complement(9228..9476)
                     /locus_tag="VFQ79_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142221.1"
                     /translation="MRNTKARDPKQTAADCYAERHAEAQDLLKRIAGRLADHQKHQAN
                     EPADWGYAGDLGRITEQLAYVLADLGDRSAVDAKGLEY"
     gene            9662..10054
                     /locus_tag="VFQ79_21045"
     CDS             9662..10054
                     /locus_tag="VFQ79_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142222.1"
                     /translation="MRGGKVNAAASDAVLDDRPEPVSERVVTTPADAAPSVTTFAQAK
                     TADMVFKAKLRKMEYDVRMGKLVEAELVKQRWSTMYRLIVDRILAWPNRLAPEVAALT
                     DERQVRETILREARALVNDLRTDVQYAR"
     gene            10044..10964
                     /locus_tag="VFQ79_21050"
     CDS             10044..10964
                     /locus_tag="VFQ79_21050"
                     /inference="COORDINATES: protein motif:HMM:NF017673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEU0142223.1"
                     /translation="MRVEEIQILAADVLAPPPDLTVSSWADQNRRLSSESAAEKGEWR
                     TDRAPYQRAVMDVMGPASPYETVVMMWAAQSGKSSMLENFLGYVIELDPGPVLLVEPR
                     EVDAEAFSKDRLAPMLRGSRVADSRSRDSNNTILHKKFLGGSITLAAANSPAGLAMRS
                     IRYCLLDEVDRYPASAGSEGDPVNLAITRTANFWNRKIVLCSTPTTKGASRVEQAWLQ
                     SNQQSYWVPCPHCAGFQILRWENLIWPKSEPEKAEYRCEHCARMIADWQKHAMLRCGE
                     WRAARPEVAGVAGFWINGLYSPWRKWARWR"
     gene            10937..11860
                     /locus_tag="VFQ79_21055"
     CDS             10937..11860
                     /locus_tag="VFQ79_21055"
                     /inference="COORDINATES: protein motif:HMM:NF042573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HEU0142224.1"
                     /translation="MAQVGALAVKFLADKRSVETLREFVNTVLAEPWDDAAETTVDQA
                     TVMARREHYRAAAPYGAVVLTAGVDVQKDRLELELVGWGRGEESWSIEYRVLPGDPSG
                     ALVWQELDTYLERRWPHETGVSLPVSACSIDAGYESQAVYEFCRTRYHRRIFAVKGKG
                     GPLPVWQRKPTAKNIRGEKPWIVGTDTAKETIYGRLKNPTPGTPGYSHFPADREEAYF
                     EQLLGEVLVTTYAKGQPKREWRPKPGVRQEALDARVYGYAALRALISMGLSLDNEADR
                     LLAANRPRPVEDDDPDRARWLGGRGKKWLSR"
     gene            11863..12036
                     /locus_tag="VFQ79_21060"
     CDS             11863..12036
                     /locus_tag="VFQ79_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142225.1"
                     /translation="MRSEPQGARGAWEYLVVTGDAESPDLLAEHGVQGWELVAVVREF
                     GTRATFYFKRRRQ"
     gene            12037..12240
                     /locus_tag="VFQ79_21065"
     CDS             12037..12240
                     /locus_tag="VFQ79_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142226.1"
                     /translation="MAWTQAQLNAIESAIASGELTVRFGDRTVTYRSMEELLQARAVI
                     KEALETESGTAKDRFSFAQTSKG"
     gene            12237..>13383
                     /locus_tag="VFQ79_21070"
     CDS             12237..>13383
                     /locus_tag="VFQ79_21070"
                     /inference="COORDINATES: protein motif:HMM:NF016991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU0142227.1"
                     /translation="MNWLDKAIAWVSPETGLRRMRARRAGELIRLAYEGARTDRRTGG
                     WVTNGNSANAEIAVALSKIRERSRDLVRNNAYAARAVAEVVGNAVGTGVTVQSRSGNP
                     EVDRAVNAAWTDWMEECDADGQLDFYGLQALVARTVFESGECLVRLRQRRPGDGLTIP
                     LQIQVLEPDYLDQTKTQRTDTGYIIQGVEFDLVGRRVFYWLYGQHPGDVVQTGVRGGA
                     SMQSLRVPASEVLHIYRKDRPGQVRGVPWLAPVVVTLRDLDEYEEAELVRKKIEACFA
                     AFVTQPQGPDGPPIAPATPDSATGKRVESFEPGMIEYLKPGEEITFASPSTSAGYRDY
                     VAAKQAQIATGLQLTYEQLTGDLSRVNYSSYRAGLLSFRNGIEAFRWL"
BASE COUNT         2491 a         4001 c         4340 g         2551 t
ORIGIN      
        1 tggatgcgcg aaacaggggc ggaggtttcg ggaggagacc acacgggagg gcgccgcagg
       61 attcgagagc ccgggtcgta caaaatgcag ccaggatcga gtggcgagac gatatcttcc
      121 agatggaagg cgccgccgta acggcccgat gcgcccatgg cttcaaagac gcgtatgaag
      181 tgcggcagaa agacggcgcc gtttccgatc agagttacgc gggcggtcat agccgtgccg
      241 ggggacaaga gggtggccga cgccgcaggt ggcacgagca caaacggatg cggcgcattc
      301 gggtacttac gcagcagggg atgccgtgcg ggatttacag gcgttacaaa taccatcgaa
      361 taaccgcagc ggtccatcac agggcaacca gggcattccg gcatatttgt cgagcagaat
      421 aggctgtgaa aaaactgccc gaaaccgccg cggaagagcg ctcccttata ttcgggcgcc
      481 cggaacagtg tccgggtttt cagacggaaa tcgagcggga caattgttaa agattgcagt
      541 ctgcggtaca gcaccattcc gaagttccgt tcaagacttg tatcacaagg gctgagatcc
      601 cgcaatcaaa ccggcacctt tgagcgacag ttggccagaa ttaagtacta aaagttccat
      661 atgaccgaaa tctgaggctc atggctggcg agaatgcgtg aatggtgttc gcctcattgc
      721 gattcattcg tagattacgg ctattactgt gccggtagtt ggatatttac tttcgccgca
      781 ttgagcacgt ccagccatag aggggcacgg cggtaaaggc ggcgcctccg gggcaagcac
      841 gatggtctcg cctgcaccga cggctgcgct attgcgttca gctgcaaagc tgtgcgctgc
      901 ggatcccgtt tcgagccgcg cgctgctgcg ccctgtggct ccaatctctt tccaataaca
      961 ccgatgtgag gtaaacccat ggcgatcaaa gagcggctcg aactgtccgc acgcatcctc
     1021 gcgagcatcg atgggcgacg caatcacaag ggcgatcatt caatattctc gtccgtagag
     1081 cggcaggtta ttgatcgcga actgaaagaa ctggcggagg aatgggctct gaatcccgac
     1141 tcgccggcag tgcccgggat ctggaggctc cgatgatttg cgatttctgt cacggaccgt
     1201 atcccgtgtg gcggtttcgc gcacaaccgt ttgtcgttga ctatggaatc ggcctgcaaa
     1261 gcgcttcgga tgcggattgg gccgcatgtg acgcgtgccg cgagttcatt cgggccggcg
     1321 accgcactgg gctcgtggat cgcgccatgc gagttgctcc gcgaatcccc ggcgccaacg
     1381 aaagcgaagt ccgccaactg cgctcctggg cgcatagtct attcttcggt catcggctcc
     1441 cgggcgaacc tttgcgaatt gaagcgagct ggtctcgatc ggctttcgac cgatgagatc
     1501 gaagcggaga tcgcggcagc ccggaaggca cggcgcgaat ctcagcccgc gcatgatttg
     1561 ggtcgttctc gacacaaaca tcatcgtctc cgctttactt cctccagaag cccttcaagc
     1621 tccactacca gatgcagtga aagagaagga accagatccc atgattcgaa ataccgagga
     1681 cttcgtgtcg gcattgcagg cagcgcgccg ggctgcaacc ccactggtcg ctatacgcac
     1741 ggcggacccg gccagcgcga tcgccctcgt acagcgcagc ctcaacggcg cggtcgagtc
     1801 gacaccgctt gtgtcctggg acatcatgac cggattgaca gcgctgaatc aggccggcaa
     1861 ggcgatgttg gacgacatcg ttggaaatgc ttctctcgcg tgtttcggac cggcgagcgt
     1921 tctggaagtg gctggcaggc tgacagacga cgcaatcctg ttttacgcga atccgcagcg
     1981 ggtttggcat gaacccgcgg tcgtccaggg catctggaat ctgcgcgaca cgctgaagac
     2041 ggtgggcgca atgctgacaa tgctggcacc tccgggcgta gtcttgccgc ccgaactcag
     2101 ccaggacgta ctcgtgatgg atgaaccgct gccatgccag gatagagccc gaagccactt
     2161 cgggccgtca cgaatacgtc accgccggaa tcaccttgac gcacggagcg tggatacagc
     2221 gttgccgcgt gctcaagaac ctcgttctcg tcgagcgccc acggggcgat cagtcgctgg
     2281 ttgctctgct gttggatggt ttcctgtttg gtcgcgtcca agtagtccac ctgaacggcg
     2341 agttccctcc gcatgaaatc gaggagtgtc ttggcaattc tgcagcgggt caccggcgaa
     2401 gcgccggcca aggcaggatg caggttctgc caggatgcaa catctttcga aaggtcgtcc
     2461 agggacaggt aatcgatctc cagaagcccg cactgctcca gattcgggga cgtcacccgc
     2521 caaccgcggc ggagatcgtg atagaggcgg tatccgatca cgtctcggaa cgcccgctcc
     2581 gtttgaagct tagcctggaa cttcaggtca ggatccttgg agtagctcgc gaacggaagc
     2641 ccaagtgcgt cgaataccct ctgggtgaag acctcgtggg tcagaccctc gggtttcgcc
     2701 gcggagcagg ccttcagtag agcggatcgc aaaagcccga tctcaacgaa gtcattgaag
     2761 tgccctgcct ggagggacgc gtcctggcgg ttgtctgtga agctcagcag ttttcgcgcg
     2821 cgtggcgcaa gggatccttc cttccggagg tgccgaatcg tggccaggct gaggatcgtc
     2881 gtggcagtgc ttctgccttc ggtaccgagt gacgtcagct tcccgaagtc tgaggtggta
     2941 cggaagtcgt aagagacacg gcaatgcaga cagaaacgga atgggctctc aacgaaatgg
     3001 caactgatgc cgtcgaccat ctcccttcca tcaggggaga ccgttactgg ccgaggcaga
     3061 ttcttcttgc gcgaagcctt cacacgcgga ccttggttcg tgtcttcgac ccagtcctct
     3121 ggcaatttcg caagctcctc gtcggtaccc gagggccacg acctctctgc gctgaaatgc
     3181 aggaatccgg ccttcgatcc atcgccaccg ctgcgatcag agagctcccg cggaatatac
     3241 gtgacggccc cggaatctga atcctccaag ctgcgcacgc agtagtattc ctgtccgcac
     3301 tcgcggcaga acaccatcgg cagcagaatc ttgcgcctgt cccccggcac gaactgctgg
     3361 cggttgaacg tcaggtagcg attgctctcg gattcgagag atgcgtagac agtgtcgcca
     3421 cggctgatga attggtgcag tcgaaacgcg aacgctggaa agccggtttc cggattccgc
     3481 tcgcaggtat agccagcgag cagcgcctcc tgtatcaccg ctttggcctt catttcgtca
     3541 gcacctgtaa cgcggctcaa ttcgcctgcg gcgccgtgct ggccagtaat ggttctgggc
     3601 cgccgacggc gcagccggtc cgaagcattg tcacggtaaa ccccgaatgt cccttcgatc
     3661 caggatgaaa gagggtctgc cgtgaatgcc tggtaagtca gtggcgatct ggaaccgtcg
     3721 ttctccacgc gctcacgcag tgagttgaga tatacgggat cggcgatgtt ctcctcgggg
     3781 gtagctcgtt gaagcgtttc gccgacgacg tcggacggag ccacctctgc gccgaataaa
     3841 acggtggcga cggccgcaac ctccgcctgc tgctgatcgt aggtccctgg gcccgccaaa
     3901 gtcgctgagg ttccgaccac ctgaagcttc ctcgcgccga aaaactcccg agcgcgtcga
     3961 acgagcagcg ccacgtcggc gccctgccgc cctcgatagg tgtggagttc atcgagtaca
     4021 aggaagcgca gcccctgtgc cgccttcacg agctgttttt cgtagaccct cgttaggatc
     4081 aactccaaca tcacgtagtt ggtaagcagg atgtccggcg gatttgcgac aatcgcgttg
     4141 cgttcctcat cgccctcttg ccctgtgtac cgggcgaacg tgaccggacc tttaccgtct
     4201 ggatagccga gcttcaagaa cttcccgagt tcgccgaatt ggctgttggc cagcgcgttc
     4261 atcggataga cgacaatggc ctgaatgccg cgcccagtgc cgttccgcag aacgtgatca
     4321 acgatcggaa tgatgtaggc gaggctcttg ccggatccgg tgccggtcgt cagaacgtag
     4381 tttcggccgg cgcgcgcccg acgaatggca tcggcttggt gctggtagag ccgtagcggc
     4441 ttgccgtcgc ctttttcctt ggcagcgcga aagactcttg agcactcggc gtggaggagt
     4501 tcctcgccaa ccaaatcgtc gatggtcccg gcgtactgga aggacgggtt cagctggatt
     4561 aacggatctg gccagaggag gccttgatcg aggtacgagg atacgagtcc ctcgatccgt
     4621 tcgtctcgaa tccggatgaa gctgcggata tacgacgagt agtcattgat taagcgctcg
     4681 cgaagctcga atacgttcat gggtggcctg gccgtccatt tcggttctca tcgggcactg
     4741 agccgttccc ggccgtcgtt cgccaaaggg caagagcatg cgtccctgcc gccctggcca
     4801 aaggcgctgc gcaacggaca gcgccatccc gaatgtctcg aagttaacca ttctatttct
     4861 attcgttgcg tccggccagc gctgcgatct gcgatcgtat tggactatcg ggcgactcgg
     4921 ttgatgccgg gttgggctgg gccgcttggc cgatgggcct gcggaggccc ccgggcggct
     4981 ggatagggcg gtaggaaggg cggttggatg cggttgcatc atgaagcagc aagtgggttg
     5041 cgcctggtgt atgattggcc tgctgtctct gatcattgaa aactgaacca tagcgatatt
     5101 ccgctttcac ggtcaatgca gtacctgctc tgtagtggcg attcagatgt aacgcgatac
     5161 ctggggtgtc ccgcacggcg aacgttgtgg atcctggtcc ccggccgccc cttcccggtc
     5221 ggcgagagga gtggctgtgc cttagcccat tgtcatttca catagcggcg aatagccgta
     5281 acgagcgccg acgagacctc cactggtcac ggcaagccgc gggccgaggt ggagaatcca
     5341 ttcgaaagcc gacatcttgg ggagccaatt catttttcag caccttcccc agcccattct
     5401 ttaacccctc tctccgtccg caactccgca actggccgtg ttttcggcac ttccgcgtct
     5461 ctccgttttg agccgactcc cgcctgaaac gccagccgtg cgccccggcg acgtttgcgg
     5521 gaggccgttt ctccccgtct ccgggcggaa accaggaatt ggttaacggc tcggtgcgga
     5581 ttgtccgacc tcaggccgga cgattgaaaa ctctgcaatg gtaggagata agataacctt
     5641 ttggtccgga gtggagcaga gcaccatggc agagtcggtc ggcgaacggg tacccggagt
     5701 gctgcttcag gcgtcgcgct cggtgtgtcg cttccttgaa gaagtccttc cgacgctgtc
     5761 ggcgtcctgg tgggctgacc tggtcgttgc gaacctatcg ttccagcagc ggcgcgccgt
     5821 ggagtctcga cgcatcgccg cactctccgg cctggaccta gccgcgctgc tccgggtgct
     5881 ggatgcgaac tggcacggca tcgctgaccg cctgagcctc ccgagtgagg cacggcacta
     5941 cctcaaggag atgcgaacgg tgcgagaccg ctgggcgcac gcctccgctt cggagttcag
     6001 tcccgacgac gtctaccgtg acctcgacac gctgcagcgt ttcctagtct ccatcggcgc
     6061 tgatgaggaa ctcctcggag aggtgaaacg gctgaagggt gcgtcactcg ccgcaggagg
     6121 tcagatgagc tccacgccag aaactccggc gccaccactg gcttcagcgt tctccgtagg
     6181 ccaagtggtc tgcctccgat cgaatcccga cacgaccgga gcggtgattg gtatccatcc
     6241 cggccaccct gaggcgcggt actcggtctt tcagagcggc acgatcgcga cgtactacga
     6301 gtcacagctt cgagccgccg acgtcgctgg tggtctcgag gtcatctcac ttcaggaatt
     6361 cgacgcatac ctgtcggcac tgcagatacg ccacccgagc acgtcgattg tgtactccct
     6421 gaacgcagcc cgcatcgatt gcgtccccta ccagttccgc ccggtcctga agctcatccg
     6481 ggccgagcga ccaaggctgc tcatagccga cggcgttggt gtcggaaaga ccatagaggc
     6541 tgggctggtc ctcaaggaac tgcaggcgcg tacggacatc cagcgcgtcc tcgtcatttg
     6601 ctcgaagccg ctcatcacgg agcggaagtg ggaatcggag atgaagcgat tcgacgagcg
     6661 gttcatccca ctggacggcg acaagctccg ttactgcgtc aacgagaccg acctggacgg
     6721 cgtgtggcca gaccagtacg caaaggccac ccgacgatga agccagtgga gctggtcgag
     6781 cgggcggtac ggaactcgag caagagccgc gacaccgtgc tcgatccgtt tggcgggtcc
     6841 ggctccacgt tgattgcctg tgagaaaacg ggccgccatg cgcgcgtgat tgaactggag
     6901 ccaaagtact gcgatgtcat cattcgccgt ttccaggaat tcagtggcaa gccggtgact
     6961 ctcgaatcgg acgaacggaa gttctccgag gtggccgcgg agcgattgca ggtggccaca
     7021 tgaatcgaac gttgccgccg ggagcttgcg gccgtcgaag cgctgctgcg cgcagaacac
     7081 ccagacgtgc aagggttgtg cctggcgctg tcggactggt ctgccgagct tcgcatcctt
     7141 gaggcgttgc catggaaaag aacattcttc aacttctcat ccccggcatc ggattggtct
     7201 cgggactgat cgccacgtac gtcagcctct agaaccgggc gctgttggcc gaagtccgga
     7261 gggagatcgc agaaatggaa catcggcttt tcttgaagat caacggcacg tacatccgtc
     7321 ggcgcgagtg tgagttgatc gagcaaaaca tgaacaagcg ggtggagtct ctcgcccagc
     7381 gggtccagga gagggaaacc gccggtgatt agaggagcac ctcaacaccc ttggcgcgaa
     7441 ccacattgag caaggcgaga gccggaccgc tgggtcgttt ggcgcctcgc tccagctgcg
     7501 acacatagcc ggaagtgaga ttgagataac gggcaaacgc ggcctggctc agtttcgctc
     7561 gctctcgaat ctcacgaatc tcttccggag tgatcggacg ggaagtgcga agcggcgcct
     7621 ctccgagatg acgaacggtg atcttgtgga aattatcgtc tttcagaacg ccaacacggt
     7681 gcattccggc ggcagtttca agcagtgcct tcgcgagccg gctagatttc ttcttcgtca
     7741 tagggaacct cctgcaattc gccctctgca agtgcacgcg caatctgtct ggcatcggcc
     7801 gcaagccagg gcgaggcgat ctcccgagcg gtcagcagtt catcggcgcc gatgttcgct
     7861 cgctcgttct tggcaaagcc atacagaaat atggcccgtt ttttcaaccg gtaggcgatc
     7921 agcattcgat agccgcccga gcgccctttg ccgcgcctgg cgacacgctg tttgatcagc
     7981 ccgccgccca gatcggcatc gaccaacccg cgctcggccc gcgcgatggc ctcgtggagg
     8041 ctggcgtcgg cgattccttc gtgcttggca aaccgcgcga gccacttggt cttgaacacc
     8101 tgcattgagc aaccctaaca ctataaacta tagcacaaaa tcatatagag accgtgcagg
     8161 gagacgggga tggggcaaac gggaagccgc cggattgtga ggcgggagtt gtctactgcg
     8221 cgatacggta ggcgcggtcg ccaccgtcgg acttgaagga ctcgaccttg atgcccatct
     8281 tcttggagag gccgccggag atgaagccgc ggacgctatg tgccagccag cccgttgcgg
     8341 attggatgtc ggcaagcgtg gcgccttcgg ggcggcgcag catgtcgagg acgatggcct
     8401 tcttgctgcc ctcgcgcgcc tccttgggcc cggcggcgtc gcctttgggc ttggcctcct
     8461 tggtcgcctt ggccttcttg gtcgcgacgg gggcggcgtg ttgcgcgggg gcgggcgtca
     8521 gggcctggac cgccttccag atgcgggcga ccgccgtctt gcggtccgtg aacttcttga
     8581 ccggcttcag cgcgtcgaag gggaccacgc cggcaaaggt gttccaggcg tcgatgaagc
     8641 ggccgatcgg ccactgactg gtcagcttgg ccagttcctt ttcgctggcg aaggttcctt
     8701 cggtgcttcc gatgcggtgg ttcagcgcat cctcgtactc ggcaaacgcc gtgatgttgt
     8761 tctcggtgtc gatcgtaaac gtagccatgg ttgttctcct gtcctatcgg ttcatgccgg
     8821 cgagtttgcc gtcggcggtt agctgcaagt ttttgaggta gccgctggca atcctggccc
     8881 acccgaatgg agtagcgatc tcatgccgcg cggcgatgcg gctcaacttc agccggtgcg
     8941 tgccgttgtc gaattccttc ttgaggtggc cccagcggtc gggcttccag ccgttttgca
     9001 tggcccaggt ggtcagttct tcgcgcgtca tcggcatggc gtcagtcctc ccgccagcca
     9061 tcgcggaggc cgctggcgtc ctcaaccgag gcgcggatgt cgttccagca gtcttcgcag
     9121 tagttggccg agtcgagcag ttcgccgtcg gccttggtga gcgtaagcgt ccgatggatc
     9181 ggtctgggtt cttcgcagag tgtgcattgc atggtggttc tccgtggtca gtactcgagg
     9241 cccttggcgt caaccgcgct ccggtcgccc aggtccgcca ggacgtaggc gagttgctcg
     9301 gttatgcggc cgaggtcgcc ggcgtagccc caatcggcgg gctcgttggc ctggtgcttc
     9361 tggtggtcgg ccaggcggcc ggcgatgcgc ttcagcaggt cctgggcttc agcgtgccgt
     9421 tcggcgtagc agtcggcggc ggtttgtttc gggtctcggg ctttggtgtt cctcatcgcg
     9481 aacccattca tcacttcggg tgcgcgaaga agcaagcgga atcgaacaat tgaatctctc
     9541 gacgtttcaa acagatcggg gcacggatgt cggataggct gatgagccag gccgagtacg
     9601 cgcggcaccg gggcaaagcc gccagtacat cagccgtctg gccaaggctg gggcgctggt
     9661 gatgcggggt ggcaaggtca atgccgccgc ctcggacgcg gtgctcgacg atcgcccgga
     9721 accggtatcc gaacgtgtgg tcaccacgcc cgcggatgcc gcaccctcgg tgaccacctt
     9781 cgcccaggcc aagaccgccg acatggtctt taaggccaag ctccgcaaga tggagtacga
     9841 cgtgcggatg ggcaagctgg tcgaggcgga actcgtcaag cagcgctggt cgacgatgta
     9901 ccgcctgatc gtcgaccgga tcctcgcctg gcccaaccgc ctggcgcctg aggtcgcggc
     9961 gctgaccgac gagcggcagg tgcgcgaaac gattctgcgg gaggcacggg cactggtcaa
    10021 cgacctgcgc acggacgtgc agtatgcgcg ttgaagagat ccagatcctc gccgccgacg
    10081 tgctcgcgcc gccgccggat ctgaccgtct catcgtgggc cgatcagaac cggcggctgt
    10141 cgtcggagtc ggctgccgag aaaggtgaat ggcgcaccga ccgcgctccc taccaacgcg
    10201 cggtgatgga cgtcatgggg ccggcgagcc cttacgaaac cgtcgtgatg atgtgggcgg
    10261 cgcagtcggg caagagttcg atgctggaga attttctcgg ttacgtcatc gaactcgacc
    10321 ccggcccggt gctgctcgtc gagccgcgcg aggtggacgc cgaggctttc tcaaaagacc
    10381 gtctggctcc gatgctgcgg ggcagcaggg tggccgactc ccggtcacgg gattcgaaca
    10441 acacgatcct gcacaagaag ttcctgggcg gcagtatcac tttggcggcg gcgaactcgc
    10501 cggctggcct cgccatgcgg tcgatccgct actgcctgct tgacgaggtg gaccggtatc
    10561 cggcgagcgc gggcagcgaa ggcgacccgg tgaacctcgc catcacgcgc acggcgaact
    10621 tctggaaccg gaagatcgtg ctctgctcga cgcccacgac caagggcgcc tcgcgcgtcg
    10681 agcaggcgtg gctccagtcg aaccagcaga gctactgggt gccgtgcccg cactgcgcgg
    10741 gtttccagat tctgcgctgg gagaatctga tctggccgaa gagcgaaccg gagaaggccg
    10801 agtaccgctg cgaacactgc gcgcggatga tcgccgactg gcagaaacac gcgatgctgc
    10861 gctgcggcga gtggcgtgcg gcgcggcccg aggttgccgg cgttgccggc ttctggatca
    10921 acggcctcta ctcaccgtgg cgcaagtggg cgcgctggcg gtgaagttcc tggcggacaa
    10981 gcgatccgtc gagacgctgc gggagtttgt gaacacggtg ctggccgagc cgtgggacga
    11041 tgccgccgag accacggtcg accaggcaac ggtgatggcg cgccgggagc actaccgggc
    11101 ggcggcgccg tatggcgctg tggtgctgac cgccggcgtc gacgtccaga aggaccggct
    11161 agagttggaa ctcgtgggct ggggacgcgg ggaggaatcg tggtccatcg aataccgggt
    11221 gctgcccggc gatccctctg gcgcgctggt ctggcaggaa ctcgacactt acctggagcg
    11281 ccggtggccg cacgaaacag gtgtctccct ccccgtctct gcatgctcca tcgatgccgg
    11341 gtacgaatcg caggcggtgt atgagttctg ccggacccgg taccaccggc ggatcttcgc
    11401 ggtgaagggc aagggcggcc cgctgcctgt gtggcagcgc aagcccacgg cgaagaacat
    11461 ccgcggcgag aagccctgga tcgtgggcac ggacacggcc aaggaaacga tctatgggcg
    11521 tttgaagaat cccacgccgg ggacgcccgg ctactcgcac tttccggcgg accgcgagga
    11581 ggcgtacttc gagcagcttc tgggcgaagt cctggtaacg acgtatgcga aggggcagcc
    11641 caaacgggaa tggcggccga agcctggcgt caggcaggag gcgctcgacg cgcgagtata
    11701 cggctacgcc gctctgcgcg cgctgatctc catggggctg tcgctcgaca atgaggccga
    11761 ccgcctcctg gctgcgaacc ggcctcgccc ggtggaggat gacgatccgg atcgtgcccg
    11821 gtggctgggc ggacggggaa agaagtggtt gtcgcgatga aggtgcgaag tgaacctcag
    11881 ggcgcgcgcg gcgcatggga atacctggtg gttaccggag acgcggaatc accggacctg
    11941 cttgccgagc acggcgtgca gggatgggaa ctggtggccg tggtgcggga gttcggcacg
    12001 cgggcgacgt tctatttcaa gcggaggcgc cagtagatgg cctggacgca ggcgcaactc
    12061 aacgccatcg aatcggcgat cgcgagcggc gaactgaccg tccgcttcgg cgatcgtacc
    12121 gtcacctacc gctcaatgga ggaactcctc caggcgcgcg ccgtgatcaa ggaagcactg
    12181 gaaacggaat ccggaactgc caaggaccgc ttctcgtttg ctcagacctc aaaaggatga
    12241 actggctcga caaagcgatc gcctgggtgt cgcccgagac gggtctgcgc cggatgcggg
    12301 cgcgccgtgc cggggaactc atccggctgg cgtatgaggg cgcccggacg gaccgccgca
    12361 ccggcggctg ggtgacgaac ggcaattccg ccaacgccga gatcgctgtg gccctgtcga
    12421 agatccgcga gcgctctcgc gatttggtcc gcaacaacgc ctacgcggcg cgagccgtgg
    12481 ccgaggttgt gggtaacgcg gtcggcaccg gggtcacggt gcagtcccga agcggcaatc
    12541 cggaagtgga ccgcgcggtg aatgccgcct ggacggactg gatggaggag tgcgacgcag
    12601 acgggcaact cgatttctac ggactgcagg cgctggtcgc gcgcacggtg tttgagagtg
    12661 gcgagtgcct ggtgcgcctg cggcaacggc ggccggggga cggtttgaca attcccctcc
    12721 agatccaggt gctggagccg gattacctcg atcagaccaa gactcagcgg accgataccg
    12781 gctacatcat ccagggggtc gagttcgacc tggtcggacg ccgcgtcttc tattggctct
    12841 acggtcagca tccgggcgac gtggtgcaga cgggcgtccg cggcggggcg tcgatgcagt
    12901 cgctccgtgt accggcgtcc gaggtcctgc acatctaccg caaggatagg cccggccagg
    12961 tgcgcggtgt gccgtggctc gccccggtag tcgttacgtt gcgcgatctc gacgaatacg
    13021 aggaagcaga actcgtgcgc aagaagatcg aggcctgttt tgcggcgttc gtgacgcagc
    13081 cgcaaggtcc ggacggcccg ccgatcgcgc ccgcgactcc ggactcggcc accggcaaac
    13141 gtgtggagag tttcgagccg ggcatgatcg agtatctgaa gcccggcgag gagatcacgt
    13201 tcgcctcgcc gtcgacgtcc gctggatacc gcgattacgt cgcagccaag caggctcaaa
    13261 ttgccaccgg cctgcaactc acctacgagc aactgactgg cgacctctcg cgcgtgaact
    13321 actcttcgta ccgcgcgggc ctgctgagct tccgcaacgg catcgaggcg ttccggtggc
    13381 tga
//
LOCUS       DASTMK010000510        22234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_314744, whole genome shotgun sequence.
ACCESSION   DASTMK010000510 DASTMK010000000
VERSION     DASTMK010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 22234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22234
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314744"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFQ79_21075"
     CDS             complement(<1..799)
                     /locus_tag="VFQ79_21075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0142228.1"
                     /translation="MRSLKDKAVLITGAGRGIGKRLALGFAAHGARVGLLARSKPELD
                     LVNLEIEHAGGVALRIRADVRDLEQMTAAVERVKTHFGGVHVLICAAAIQGPIGPFLE
                     NPVKQWTETVETNLIGVMNSCRAVLPRMIERRSGKIIVLSGGGATKPRPFFSAYAASK
                     AAVVRFTETLADEVRDHNIQVNAMSPGGTYTHMTDEILRAGDSAGWKDRDDAVKLRLT
                     GGVGPEQQLALAVFLASEKSNHISGKLIHVSDNWKKLESSGVEPDLFT"
     gene            complement(876..951)
                     /locus_tag="VFQ79_21080"
     tRNA            complement(876..951)
                     /locus_tag="VFQ79_21080"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Thr,seq:tgt)
     gene            complement(1038..1469)
                     /locus_tag="VFQ79_21085"
     CDS             complement(1038..1469)
                     /locus_tag="VFQ79_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142229.1"
                     /translation="MRDREKQMKITDQFKEWAARLAAMEPGADPFVSCYLDLSPGFLT
                     RFDSSARAAKKGLRPRERRAVTDALARVESFLKIDAGRGEGGVAIFSRAGVDPFFLTL
                     PLDVPVSTICTIAPRPDTSGLLALTEQGDSAGFEEVAAPAY"
     gene            complement(1459..1977)
                     /locus_tag="VFQ79_21090"
     CDS             complement(1459..1977)
                     /locus_tag="VFQ79_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142230.1"
                     /translation="MKRDEILDLGLPHSRETVRRLAMVHNVSAPDLKLADVLTISPNP
                     TLSVYFSGLFDQFGWAVVRRDSCESAIDFLRRNRAAVAICEEELPDGGWKSVIRAAGA
                     MPDPPSFVVIGEEAPLWKEVSRLGGFDVLTRPLREADVVWTVASAWHHWMKQTEMRDQ
                     QQEEERPVIHAG"
     gene            complement(2070..2468)
                     /locus_tag="VFQ79_21095"
     CDS             complement(2070..2468)
                     /locus_tag="VFQ79_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU0142231.1"
                     /translation="MPNRVNPKINKEAFRSRLRAEKAKLFSGRRDGNLEVLTLTAGAA
                     AEDHAPLVHDQFVAVRTHRIHGEKLKLIDAALRRLEEGAFGECEDCGRPIALKRLEAI
                     PWASRCVPCQERAVDNTAAEEAENHFKMIA"
     gene            complement(2505..3734)
                     /locus_tag="VFQ79_21100"
     CDS             complement(2505..3734)
                     /locus_tag="VFQ79_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0142232.1"
                     /translation="MSFDPLCWFALLLAAVPDSLNIPLTMLLVFGSAKLLAELFEKFH
                     QPGIVGEILAGILLGPSILNWLQPTNFLAALADLGVMFLLFRVGLEVKSSELMKVGAP
                     AALAATLGVIVPFILGYGIITLWGGPTVEALFVGAAMVATSVGITAQVLSSKGVLRHT
                     ASQLILAAAVIDDILGLLILALVSSMAKGQVNVLELLLTSVLAIGFTVLVAKWGTQTM
                     GRFVPGIEKRLRSGEVQFNVAMVLLFALSLLAMRAGVAAIIGAFLAGMALSETVNHRV
                     HDLAHGVTELLVPFFLVGIGLHFDLAAFSDSSTIVLALVILAAAIVSKLVGCGLGALS
                     LGWPNAIRVGVGMIPRGEVGMVVAQIGLGLGVIPKPVYGVVVFMAILTTMLAPPLLNI
                     AYRGVQPVAPAEEFRLG"
     gene            complement(3731..6529)
                     /gene="treY"
                     /locus_tag="VFQ79_21105"
     CDS             complement(3731..6529)
                     /gene="treY"
                     /locus_tag="VFQ79_21105"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEU0142233.1"
                     /translation="MRIPTATYRAQMNKDFGFQAARRALPYLTGLGISDLYMSPILKA
                     RPGSTHGYDVVDPTALNLELGSEEDFRALVEDLKQRGMGLLLDIVPNHMAASSDNPWW
                     RDVLENGASSWYAGYFDIHWERNPGQASEEQIFLPILGAPYGTVLENQELRLAMEPDG
                     FFVHYYAAKLPVGPTFYDDILKYRFDGAHQGLAALIEQIERLPERTVTDWAALEARQH
                     DKERIKRELWLLYSSDPGVKEFIDENIRIFNGEKGSPSSFDLLDHILAGQPYRLSFWR
                     VARERINYRRFFDVTDLIGLRAEEPGVFESTHAFILELMSKGLITGLRIDHIDGLYDP
                     LAYLTQLKSRITDEDAYIVVEKILTGNERLPEQWPVAGTTGYDFLGFSNSLFVEPAGF
                     AEFQRIYARFTGIRDKFEDVVYQKQKFVMDELFPGDILALGAHLALLAEHDRHARDLS
                     PRELSAALLATTACFPVYRTYTRDFNVSPEDRACIEQAVATARTRNPEITPAVFDFVR
                     RVLLLEFPEWLPEDQPGNWLAFVMRWQQLTSPITAKGIEDTTLYMYNPLVSLNEVGGL
                     HHPVSPEEFHEFNRRRHERWPLTMNATSTHDTKRSEDVRARINVLSESPWEWARSIYR
                     WSRFNEGKKLVPPGQPRVDANEEILIYQTLVGGWPLCDEDIPAFRERMKQYILKAMRE
                     AKVYSSWIEPNVPHEEAVISFLEGILEPDPGNRFLPDFLKFQSEIAWYGALNSLAQLL
                     LKITSPGVPDFYQGAGLWDLSLVDPDNRRPTDYERRTAMIADIEARSQGALAKFAAEL
                     LKDWKSGAIKLYLSYQGLQLRRRLPDVFNRGGYLPLDVLGRRRDHIISYARSHNGAWV
                     VTVVPRLLTEFTTSFKPPVGFRIWRDTAIEIPAGGPCHWRNVFTNEDLTSSPCGEGAL
                     LKLEHVFSILPFAILESR"
     gene            complement(6537..8963)
                     /locus_tag="VFQ79_21110"
     CDS             complement(6537..8963)
                     /locus_tag="VFQ79_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEU0142234.1"
                     /translation="MRYICIHSHFYQPPRENPWLEAIELQDSAYPYHDWNERISAECY
                     APNAAARILDGEGRIVRIENNYSRISFNFGPTLLSWIESQAPDLLADIVEADRASRER
                     FSGHGSALAQAYNHMILPLSNCRDKETQIVWGIRDFEHRFHRPPEGMWLPETAVDLES
                     LRLMAKHGIKFTILAPHQARRIRKIGGRSWHDVHDARIDPTMAYRLRLPDNSTIALFF
                     YDGPVSRAVAFERLLNNGEDFAGRLMSVYSDARTWPQLAHIATDGESYGHHHYHGEMA
                     LAYALHHIEAKGLARLTNYGEYLERHPPTHEVEIFEDTSWSCLHGVERWRSNCGCNSG
                     GRPGWTQEWRTPLRSALNWLRDCVMPLYEEAGARLFRDVWAARNAYVDVILDRSPGSR
                     ELFLSRHAVDSLTPDDQTIAWKLLELQRHSMLMYTSCGWFFDDLSGIETVQVIRYAGR
                     VVQLAEDLFPERQIEAQFLTHLAGARSNLAEHGNGAEIYDKFVKPTLVDLQKVGAHYA
                     VSSLFESYPESAPIYCYTAEREDYRSVEAGKLHLAIGRARFISGITQESGVLSFGILH
                     FGDHNINGAVRQYRGEEEYNELALQAKEAFFRADIPQVIRILDKGFGENIYSLKSLFR
                     DEQRKILRRILNTTLEEAGAVYRQLYEHHAPLMRFLVSLGIPLPKALRTTAEYALNLH
                     LKNAMAAEELDVDRIKSLLEEARAGQIELDSTTLEFTLRKSLERLALRFASSPADDRL
                     LDKLTSVVDLLALLLFDVDLWTIQNVCYECMRAVYPEMLSRAERGNSEAKNWVARFRP
                     MAEKLRLRIT"
     gene            9149..10555
                     /locus_tag="VFQ79_21115"
     CDS             9149..10555
                     /locus_tag="VFQ79_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142235.1"
                     /translation="MSAARDVSRRDAVKLAGAATVAAAASTIQGAPFISKVKAANNQV
                     PYAFIGTGGRGQYLLKHFNGIDSGRCVAVCDIYEPNLKKGIETSKDKPQGYFDYRELL
                     ARKDIEAVFITTPLYMHFPVTRDALLAGKHVFCEKSLVFRPEEIHALRKLVNERPKQV
                     LQVGLQRRYSKFYQTAKQMIDKGVIGNVTHAYAQWHRNPGWTMKEFPTRQREMNWRLF
                     REFSGGLTAELASHQIDVADWCFGATPEFVVGVGGLEYVKDGRDVYDNIQLIYKYPRG
                     QKLIYSSISTNKHLSLFQGTRTEFGERIMGTEGTIEITVGTDNEPAIGLWYSEPGPKV
                     TKEEQKKKVEAVAGATLASTGSGARALPILFERDMVTGKESFIERELKFARRWLYAKG
                     IMVPEENRNPVDIELEEFLECVRTGARPKADLEIGLADSTAVILSNLAMDEGRRVYFN
                     EIDKMGLSQPAAANSHKT"
     gene            10664..11728
                     /locus_tag="VFQ79_21120"
     CDS             10664..11728
                     /locus_tag="VFQ79_21120"
                     /inference="COORDINATES: protein motif:HMM:NF025839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HEU0142236.1"
                     /translation="MSTYRDRRAASIENNAVRVTVLVEGGHIAEICHKQSGVNPLWTP
                     PWPSIEPSTYTLEKHPEYGADSESKLLAGIMGHNLCLDIFGPPSAEEAAAGLTVHGEA
                     SVVPYRISTEGRTMTARADLPLAQLQFERRITVPEADGIVRVEETVENLSAADRPIAW
                     TQHVTIGPPFLQKGSSQFRASATRSRVHEEDLEGSRLKPGADFDWPMAPRKDEAAYDL
                     QTYTDAPESSEFTTHLMDPTREHAFFMAWSPVSHVLFGYLWKCADFPWLGIWEENYSR
                     KQPPWSGKTLTRGLEFGASPMAETRREMIDRNKLFGVPAYRWLPSKAKLRAGYCIFIL
                     TRDRIPERVSWNGTDSVEFE"
     gene            complement(11745..11945)
                     /locus_tag="VFQ79_21125"
     CDS             complement(11745..11945)
                     /locus_tag="VFQ79_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142237.1"
                     /translation="MAGRGPQSFKKRQKEQQRKERQQLKMEKRLQRREEKAAGGGAAL
                     EIAPEEDNDFAVTTEESHTHTS"
     gene            12188..13222
                     /locus_tag="VFQ79_21130"
     CDS             12188..13222
                     /locus_tag="VFQ79_21130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0142238.1"
                     /translation="MASPHTQEISYSILDLAPVRAGGSIAESFRNMLDLARHAEIWGY
                     TRFWLAEHHNMPGIASAATSVLIGYVAGRTSTLRVGSGGVMLPNHAPLVIAEQFGTLE
                     TLYPGRIDLGVGRAPGTDGLTARALRRGLNGGEDDFPKQVAELLAYLAPAGPGQKLQA
                     VPGAGTNVPVWLLGSSTYSAQLAAMMGLPFGFASHFAPELLFQAIQIYRERFKPSPFL
                     EKPYVMAGVPVVAADTDAEANRLATSPALRFLKLLRNESLFIPPPVASMDGLWSDPER
                     QIVESKLRTSVVGGPETVRRKLARFLEDTQADELIFTSDVYDPAARLRSFEIAAAAMK
                     GIRDGCAKMK"
     regulatory      13215..13277
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            13296..13676
                     /gene="crcB"
                     /locus_tag="VFQ79_21135"
     CDS             13296..13676
                     /gene="crcB"
                     /locus_tag="VFQ79_21135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU0142239.1"
                     /translation="MPLQTILWISLGSIVGGNLRYFVARLTAKLIPSDFPYGTLLVNV
                     TGSLMLGFFLVWTTERVFADPRWRLLIAVGFCGGYTTYSGFAYETFALFEQGQWASSA
                     VNLLLTNVLCMFAVILGAMLARSL"
     gene            13687..14010
                     /locus_tag="VFQ79_21140"
     CDS             13687..14010
                     /locus_tag="VFQ79_21140"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU0142240.1"
                     /translation="MNTAVQVTIYLNEYDQWHRKPLHAEILRMLREENVAGACAFHSI
                     AGFNGRNSVHTTSLVETGGNVPVIVIFVDMEEHVSRVLPRLREMAPHRLIVRENVVIE
                     QGNLD"
     gene            complement(14016..14906)
                     /locus_tag="VFQ79_21145"
     CDS             complement(14016..14906)
                     /locus_tag="VFQ79_21145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0142241.1"
                     /translation="MNRKKASDFPQELLDLFDRYVHGDIDRRGFLDGAKKFASGGLTA
                     AAIWESLRPNYAWAEQVAKQDPRIRTAYETVDSPKGNGSIRGYLVRPAKAGKLPGVLV
                     IHENRGLNPYIEDVARRLGTENFVAFAPDGLTSVGGYPGDDEKGGQLFRQVDRAKMSE
                     DFIAAAHWLKSRSDCTGKIGVVGFCFGGAISNMLAVRLGSDLSAAVPFYGGQPKADEV
                     PGIKAPLLLHYASLDTRVNAGWPAYEEALKANHATYTAHFYAGTNHGFHNDTTPRYDE
                     AAAKLAWQRTLDFFNKYLRG"
     gene            complement(15087..15401)
                     /locus_tag="VFQ79_21150"
     CDS             complement(15087..15401)
                     /locus_tag="VFQ79_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142242.1"
                     /translation="MSTSRLEALQNLLSQNPNDTFARYGLAMEYTRNGDLEKAVEEYG
                     KLLAVNPDYAAAYFHAGQTYEKMGREEDARAMYRKGIEVTTRTGDLHTRSELQGALDL
                     LG"
     gene            15435..15878
                     /locus_tag="VFQ79_21155"
     CDS             15435..15878
                     /locus_tag="VFQ79_21155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142243.1"
                     /translation="MACPFFYPVSKLDSSRRRTLPRLPLGDAYEGICRSLPQRDHVPD
                     EAALRELCSFGYARGTCSRHPAGECPDAVRISPMPGDGPLIKLIYVLERAHAPLEHGT
                     AVYSAADKRWIEAPQNEIVRRQAEVYAEGFAQRKYGVSNVLKAAC"
     gene            15892..16380
                     /locus_tag="VFQ79_21160"
     CDS             15892..16380
                     /locus_tag="VFQ79_21160"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU0142244.1"
                     /translation="MDEPIEGKPVRESVSECSEIALPNDANPLGHLLGGRVMHLVDLA
                     GAIAAMRHSRLAVVTAAIDYMTFLHPIHIGQLITLRSSVNRVFRTSMEVGVKVFVEDL
                     GTGEVRHTSSAYLTFVAIDAAGQRIRVCPVIPETAEEKRRYEEAGRRRQHRLEQRERS
                     RV"
     gene            complement(16436..16750)
                     /locus_tag="VFQ79_21165"
     CDS             complement(16436..16750)
                     /locus_tag="VFQ79_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142245.1"
                     /translation="MEAANRKLIRPSLNGMKDQMNPRRSANQSQKKSVPPEQTHAENF
                     YYTKQMQAKTPMVFVLKDGETLHGIIEWYDKCCLKINRDGEPNLLIFKPNIKYMYKAD
                     NE"
     gene            complement(16878..17927)
                     /locus_tag="VFQ79_21170"
     CDS             complement(16878..17927)
                     /locus_tag="VFQ79_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0142246.1"
                     /translation="MFDDFYGHEAVTAALDLMIRENRIPQTMLFSGAEGVGKATLARR
                     FAARLLGDPGKIEKDDLSRPENAAYVAERERWTSEKRVQDPLVFGTHPDFLTFPPDGP
                     LRQISIQQMRLLKERAQYGPLKGKRRVFLVDQIDRANEQAANSLLKILEEPPDYLVLV
                     LTASNPYDLLPTIRSRAVPFHFPPLSPDQMLVYLQNRGIDHIDRRIALASGCPGAAVS
                     IDLDSYDKRRTAMLALLQVAAGREPFALWARHSETIGASKSEKLDAYLKVLYLLIEDL
                     LVLNECGAGTAALLRNIDLKKELTPIAQSVSFDWISLATRKIDEIAAFLRRNIQKTIA
                     LDAFVVDLRAKARSG"
     gene            17936..19564
                     /locus_tag="VFQ79_21175"
     CDS             17936..19564
                     /locus_tag="VFQ79_21175"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU0142247.1"
                     /translation="MLILANRDGSASPFVKLSYVFMKLGVDFGTTRVVVAAADRGNYP
                     IVGFEAPDGAIHEWFPPLVAVRGSQRLYGWTAWRAQEYPGWTIVRSLKRYLDQGGPQT
                     EVHIADQTVLLGQLLSELMQALKSALVDRSTANLRTGEQVEVMLGVPANANTNQRFLT
                     SEAFRCADFSVAGMLNEPSAASIEFSHKRGGTNSRILVYDLGGGTFDASLVECNGGSH
                     TVVASEGITTLGGDDFDTILAELALESAGVEQEERDGMTQAEWFRLQDECRDRKEALH
                     PNTRRIIVDLERVRQEWAPVTILAADFYAACRPLVEETIHATEDLLARHAAGKEIDAL
                     YVTGGGSELPLVSRALRERFGRRVRRSAHTRSATAIGLAIQAADTEGYALKDRFTRYF
                     GVWRESEDGRSIVFDPLFDKGTGLPGPGEPPLERTRVYTPVHNVGHFRYIEASHCDES
                     GNPQGDITEWDEIVFPFDRALQELPDLSRVDVERRTAGGGEIRELYRCDSAGSVWVEI
                     SDLATGHQRTFRLGRWLRSDAPVKPGRKKKSAKG"
     gene            19575..20888
                     /locus_tag="VFQ79_21180"
     CDS             19575..20888
                     /locus_tag="VFQ79_21180"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142248.1"
                     /translation="MSKKRDGVARRQLLQVGAGGLAAAPMIVPASALGLGQAVAPSDR
                     VTVGGLGIGGRGASVLRSFLAQPDVQFRAICDVRNERREAIKSMADQQYGNHDCAMYS
                     DQEELWARKDIDALLIATGDRWHAPLSILAAKAGKDVYCEKPCSMTIAESRALADTYQ
                     RLGRIYQAGTQRRNGANFIKAYDLARSGKLGKLTALHAEAGPGDRWAPLTSHAWLPAE
                     PEPSKQVVDWDRWLGPAPWRPFNSQYIRGRWRGYFDFHGGGILEWGSHTVDLCQWVGD
                     LDDTTPVEFEPHGMGADTPYTIYGRYANGLKLVLRDKGFLGLGTCHIRFEGDAGWVET
                     ADGGKIEVSANLRGEDLAVSPQEAGRATANHVRDFVNCVKSRKQPKSNALAAAQAHIA
                     SHASYIAFQLGRKLNFDPATDTFTGGGSEEANRMRSRAMREPWRI"
     gene            20903..21445
                     /locus_tag="VFQ79_21185"
     CDS             20903..21445
                     /locus_tag="VFQ79_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142249.1"
                     /translation="MSRRNFLTGQSMMASAAAQGTAPQQPAAAKPKPEQPPVIPPMVD
                     SKYGTAEQIMSLPAARLIAILTDRDGSEYAKAKACQRLAVIGGPQAVPALAALLSDPK
                     LSHYARFGLEPNPDPSAGAALLGALDRVQGSLLVGIVNSIGNRKDKRAIAALGKLRSH
                     TDLEVARAADAALAKIRPGL"
     gene            21492..>22234
                     /locus_tag="VFQ79_21190"
     CDS             21492..>22234
                     /locus_tag="VFQ79_21190"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0142250.1"
                     /translation="MPSQLSRRELLRVAGGAGFFAFADLAPAAGTPPGVLTFRHIHLD
                     FHTSPAVTGVGSEFKAEEFARILKEAAVNSITIFGKCHHGMSYYPTKVGTRHPELKVD
                     LVGEMIEACHRQGIRVPVYISTMYDQHIWRRHADWRVYDEEGKPFGLRGPAGPLKAEL
                     GRVCVNTPYMDYVLAQAEEVLTNYEADGLFYDNFAYPAFGCLGPSCMAEREKLGLESA
                     RLEDRQKHAGMVTERSIARFAATVRARKP"
BASE COUNT         4561 a         6440 c         6770 g         4463 t
ORIGIN      
        1 gcgtaaagag atccggttca acgccggaac tctccagttt cttccagtta tcgctgacgt
       61 ggatgagctt cccgctgata tggttcgatt tttccgacgc caggaaaacc gccagcgcca
      121 gttgctgttc gggaccgacg ccgccggtga gccggagttt gacggcatcg tcgcggtctt
      181 tccagcccgc actgtcgccc gcacgcagga tttcatccgt catgtgcgtg taggtaccac
      241 ccgggctcat cgcatttacc tggatattgt ggtcccgcac ctcgtcggca agagtctcgg
      301 tgaatcgcac caccgccgct ttagaggccg cataggctga gaagaagggc cgcggcttgg
      361 tagcgccacc acccgagagc acgatgattt tgccgctgcg ccgctcgatc atacggggca
      421 acacggcgcg gcaactgttc atcactccta tcaggttggt ctccaccgtc tcggtccatt
      481 gcttgaccgg attttccagg aacggaccaa ttggtccttg tatcgccgcc gcgcaaatca
      541 gcacatgcac gccgccgaaa tgcgttttca cgcgctccac tgcggccgtc atctgttcca
      601 gatcccgtac atcggcccgg attcgcagcg cgactccgcc ggcatgctca atttcaagat
      661 ttacaagatc gagttccggt ttgctgcgtg ccagcaatcc cacccgggca ccgtgggccg
      721 caaatcccag cgcaaggcgc ttcccgatgc cgcggccggc gccggtaatc aaaaccgctt
      781 tgtctttcag gctccgcaca ccggtattaa atcagaaacc gaagctgggg attggcagcg
      841 ggaatgcgta ccatgatccg gacaaagaag gcagttggag ctggcggagg gatttgaacc
      901 cccgaccctc tgattacaaa tcagatgctc taccaactga gctacgccag caccctatgc
      961 ggccgggaac ttgaagccgt atacgtgagt ttaacatccc ctcttcgcat ccgcattccc
     1021 ctccaccgga gtcagggcta gtaagcgggc gctgccactt cttcgaaccc ggcggagtcg
     1081 ccttgttccg tcagtgccag caagccggag gtgtcggggc gtggtgcaat cgtgcagatc
     1141 gtggagaccg gaacatcgag cggcagcgtg aggaaaaacg gatcgacgcc ggctcgcgag
     1201 aagatggcca ctcccccttc gccgcgcccc gcatcgattt tgagaaagct ttcaacgcgg
     1261 gcgagggcat cggtaacggc acgccgctca cgcgggcgca gtcccttttt agccgctcgc
     1321 gcgctggagt cgaagcgggt caggaaaccc ggtgacaaat cgaggtagca actgacgaaa
     1381 ggatcggcgc cgggctccat cgccgccagc cgggctgccc actccttgaa ttgatcggta
     1441 atcttcatct gtttttctct atcccgcatg gatgaccggg cgttcctcct cctgctgctg
     1501 gtcgcgcatt tcagtctgct tcatccagtg atgccaggcg ctggcgacgg tccacaccac
     1561 gtccgcttct ctgagagggc gcgtgaggac atcgaagcct cccaggcgcg aaacttcttt
     1621 ccaaagcggc gcttcctcgc ctattacgac gaaggaagga gggtccggca ttgcaccggc
     1681 agcccgtatc acgcttttcc aaccgccgtc cggcaactcc tcttcacaaa tagccaccgc
     1741 ggcacggttc cgccgaagaa agtcgatggc gctttcgcaa ctatcgcgac ggacaaccgc
     1801 ccaaccgaat tgatcgaata gaccggagaa atacacgctg agcgtcgggt tcggactgat
     1861 cgtgaggacg tccgccagtt taagatccgg cgcagacacg ttatgcacca tcgcaagcct
     1921 ccttacggtt tccctgctgt gaggcaatcc gaggtccagg atttcatcgc gtttcacgcg
     1981 atttcgtagt tgggaccggc ggctctctgc cgcaaaaaag ccaatactgc ctgttttggg
     2041 acagtacagc ctgtgagcga cgggtacgct caggcgatca tcttgaagtg attttcggct
     2101 tcctccgcgg cggtgttatc gacggctcgc tcctggcagg ggacacagcg cgaggcccag
     2161 ggaatcgctt ccaggcgctt caaagcgatc ggccggccac aatcttcgca ttccccgaaa
     2221 gcgccctctt cgagacgccg gagcgcggca tcgatcagct tgagtttctc gccgtggatc
     2281 ctatgcgtgc gaaccgcgac gaactggtcg tgaaccaacg gcgcatgatc ctccgcagcc
     2341 gctccagccg taagggtcaa cacctccagg ttgccgtcgc ggcggccgga gaacagttta
     2401 gccttctccg cccgaaggcg ggaccggaaa gcttctttat taatttttgg gttgactctg
     2461 ttcggcatga cacttcctgt tgagcaagtg tcgtgccagc gaactcaccc cagccggaac
     2521 tcttcggcgg gcgccaccgg ctgcacacca cggtaggcga tgttcagcag tggaggagcc
     2581 aacatcgtgg tgaggatggc cataaagacc accacgccat acacgggttt cgggatcacg
     2641 cccaatccga gcccgatctg agcgaccacc attcctactt cgccccgcgg aatcatgcct
     2701 acgcccaccc tgatagcatt gggccagccg agcgaaagcg ctcccaaacc gcagcccacc
     2761 agcttggata cgatggcggc ggcgagaatg acgagcgcca atacgatggt ggacgaatca
     2821 gagaaggctg cgaggtcgaa atgcagtccg atgccaacca ggaaaaacgg aaccagcagt
     2881 tccgttacgc cgtgggccag gtcatgcacg cggtgattca cggtttccga cagtgccatg
     2941 cccgcgagga aggcgccgat aatagcggcg actccggccc gcatggcgag aagcgagagc
     3001 gcgaacagca gaaccatggc gacgttgaac tgaacttctc cggaccggag cctcttctcg
     3061 atgcccggaa cgaatcgccc catggtttgt gtcccccatt tcgcgaccag cacagtgaag
     3121 ccaatcgcca ggacggaggt cagcagcaac tccagcacat tgacctgtcc tttcgccatg
     3181 ctgctgacca gcgcgagaat caacagacca aggatgtcgt cgataaccgc ggcggcaagt
     3241 atcaactggc tcgccgtatg ccgcagtaca ccctttgagg aaagcacctg cgcggtgatt
     3301 cccacgctgg tggccaccat ggcggcaccg acgaacaatg cttccaccgt tggcccgccc
     3361 caaagtgtga tgatgccgta gccgaggata aacggaacaa tcaccccgag cgtcgctgcc
     3421 agcgccgccg gtgctcccac cttcatcagt tcggaagatt tgacttccag gccgacgcga
     3481 aaaagcagaa acatgacacc gagatcggca agggccgcca ggaagtttgt gggctggagc
     3541 cagttcagga tgctgggacc cagcaggata ccagcaagaa tctcgccgac aattccgggc
     3601 tgatggaatt tttcgaacag ctccgccagc agcttggccg acccgaacac cagcagcata
     3661 gtaagcggga tgttcaagga atcgggaacc gcggccagca acagagcgaa ccagcagagc
     3721 ggatcgaaac tcatcgggac tccagaatcg caaacggaag gatcgaaaag acgtgttcca
     3781 gcttgagcag agcgccttcg ccgcaaggag aactggtcag gtcctcgttg gtgaatacat
     3841 tgcgccagtg gcagggtccg cccgcgggta tttcaatcgc cgtgtctctc caaatccgga
     3901 aaccgaccgg cggcttgaac gaggtagtga attccgtcaa caggcgcggc actacggtga
     3961 caacccaggc gccattgtgg ctgcgggcgt aggaaataat atggtcgcgc cggcggccca
     4021 gcacgtcgag cggcaagtag ccgccgcgat tgaacacgtc aggcagcctg cggcgcagtt
     4081 gcaggccctg ataagacagg tagagcttga tcgcgccgga tttccagtcc ttcagcagtt
     4141 ccgccgcgaa cttcgcaagg gctccctggc ttcgtgcttc gatgtccgcg atcattgcgg
     4201 ttcgccgctc gtagtccgtc ggccgccggt tgtccggatc aacaaggctg agatcccaaa
     4261 gtccggcgcc ctgatagaag tcaggcacac caggggaagt gatctttaac agaagctgcg
     4321 ccagcgagtt caaagcgcca taccaggcga tttcagattg gaacttcagg aaatccggaa
     4381 ggaaccggtt gccgggatcc ggctcaagaa tcccctcgag gaaggagatg acggcctcct
     4441 cgtgcggcac gttcggctct atccagctcg aatacacctt cgcctcgcgc attgctttga
     4501 ggatgtactg tttcatgcgc tcgcgaaacg ccggaatatc ttcgtcgcag aggggccaac
     4561 cgcccaccag ggtctggtaa atgagtatct cctcgttggc atcgactcgt ggctggcccg
     4621 gcggaaccag cttttttcct tcattgaaac ggctccagcg gtagatggag cgggcccact
     4681 cccacggcga ttcggagaga acgttgatgc gagctcgaac gtcttcactc cttttcgtat
     4741 cgtgcgtgga agtggcattc atggtgagag gccagcgctc gtgccggcgc cggttgaact
     4801 cgtggaattc ctccggagaa acaggatgat ggaggccgcc cacttcattc agcgagacca
     4861 gcggattgta catgtagagc gtggtatctt cgatgccctt ggcggtgatg ggactggtca
     4921 actgctgcca tcgcatgacg aaagccagcc agttgccggg ttgatcctcc ggcagccatt
     4981 ccgggaattc gagcagcaga acccggcgca cgaaatcgaa taccgcaggc gtgatttccg
     5041 ggtttctcgt tctggctgtc gcgacagcct gttcgatgca ggcccggtct tccggcgaga
     5101 cattgaagtc ccgggtgtaa gtgcggtaca cggggaagca cgcggtggtg gcaaggagcg
     5161 cggccgaaag ctcccgcggg ctgagatcgc gtgcgtgccg gtcgtgctcg gcaagcagtg
     5221 ccagatgcgc gccgagggcc agtatatcgc cgggaaacag ctcgtccatc acgaactttt
     5281 gcttctgata gacgacgtcc tcgaacttgt cccggattcc agtgaatcgc gcgtaaatcc
     5341 gctggaactc ggcaaagccg gcaggttcca cgaacaggct gttggagaag ccgaggaaat
     5401 cgtacccggt cgttccggcg accggccatt gctccggcag ccgctcattg cctgtaagga
     5461 ttttttcaac gacgatatag gcgtcttcgt ccgttatgcg gctcttcagt tgcgtaaggt
     5521 aggcgagcgg atcgtacaag ccgtcgatgt gatcgatacg aagcccggtt atcagccctt
     5581 tcgacatcag ctcgaggatg aatgcgtgag tgctttcaaa gactccgggc tcttcagccc
     5641 gtaatccgat gagatcggtc acatcgaaga agcgccggta attgatgcgc tcgcgcgcca
     5701 ccctccagaa ggaaaggcgg tagggttggc ccgccagaat atggtcgagt aaatcgaagc
     5761 tggagggtga gcctttttcg ccgttgaaaa tgcggatatt ctcatcgatg aactccttca
     5821 cgcccggatc gctgctgtac agcaaccaaa gctcgcgctt gatccgctcc ttatcgtgct
     5881 ggcgcgcttc gagcgcggcc caatcggtga ccgtcctttc cggcagacgc tcgatctgtt
     5941 cgatcagggc cgcaagcccc tggtgcgccc cgtcgaagcg atatttcaga atgtcgtcat
     6001 agaacgtcgg ccctaccggc aacttcgctg cgtagtaatg cacgaagaat ccgtccggct
     6061 ccatcgccaa ccgcaattcc tggttttcga gaacggttcc gtatggggct cccaggatcg
     6121 gaagaaagat ctgctcctcg gaagcctggc cggggttgcg ctcccagtga atatcgaagt
     6181 agcccgcata ccaggaactc gcgccattct cgagcacgtc cctccaccag ggattgtcgg
     6241 aactggccgc catatgattc ggcacaatgt caagcagcag ccccataccc ctctgcttca
     6301 gatcttcgac gagagcccgg aagtcctctt cgcttcccag ttccaggttt aatgccgtcg
     6361 ggtctacgac atcgtagccg tgagtgctgc cgggacgggc tttcagaatc ggcgacatat
     6421 acaggtcgct gataccgagc cccgtaagat acggcagggc acgtcttgcg gcctggaagc
     6481 cgaaatcctt gttcatctgg gcgcggtaag ttgccgtagg aatgcgcata acggaatcag
     6541 gtgatacgca gtctgagttt ttccgccatc ggacggaacc gcgccaccca gttcttcgcc
     6601 tccgaatttc ctcgttcggc gcggcttagc atctcggggt agaccgccct catgcattca
     6661 tagcagacgt tctggatggt ccaaagatcg acatcaaaga gaagaagcgc cagcagatcc
     6721 acgacggaag tcagcttgtc gagcaacctg tcgtcggcgg gcgaagaggc aaaccgcaag
     6781 gccagacgtt ccaggctttt gcggagtgtg aattcgagcg tcgtggaatc gagctcgatc
     6841 tgtccggcgc gcgcctcctc gagcaggctc ttgatccggt ccacatccaa ttcctccgcc
     6901 gccatcgcat ttttcaggtg gaggttcagc gcatactccg cagtggttcg cagggctttc
     6961 ggaagcggaa tccccaggct gacgagaaaa cgcatcagcg gcgcgtggtg ttcgtacagt
     7021 tgccggtaca cggctccggc ttcttccagc gtggtgttga ggatacgtct caggatcttg
     7081 cgctgctcat cgcggaagag tgatttcaag gagtagatgt tctcgccgaa gcccttgtcg
     7141 agaatacgaa ttacctgggg gatgtcggcc cggaagaacg cctctttggc ttgcagcgcc
     7201 agttcgttat attcttcttc accccgatac tgccgcacgg cgccgttgat gttgtgatca
     7261 ccgaaatgca gaatgccgaa gctcagtacg ccggattcct gggtaattcc ggagatgaag
     7321 cgggcgcgcc cgatagccag gtggagctta ccggcttcga cggaccggta gtcctcgcgt
     7381 tcggcggtgt agcaataaat cggggcgctt tcggggtagg actcgaaaag cgagctgacg
     7441 gcgtagtgcg cccccacttt ctgcaagtcg acgagcgtgg gtttgacgaa cttgtcgtag
     7501 atttccgcgc cattgccgtg ctcggcaagg ttgctcctcg cgcccgcgag gtgggtgaga
     7561 aattgagctt cgatctggcg ctcggggaac aaatcttcag ccagttgcac aactcttccg
     7621 gcataccgga ttacctggac cgtttctatg ccggaaaggt catcaaaaaa ccagccgcag
     7681 cttgtgtaca tcagcatgga gtgccgctgg agttcgagga gtttccaggc gatcgtctga
     7741 tcatccggtg tcaggctgtc aacggcgtgc ctcgacagaa acagttcgcg agatcccgga
     7801 gaccggtcga gaataacgtc gacgtaggcg ttgcgggcgg cccagacatc acggaaaagg
     7861 cgcgcacccg cctcttcgta caatggcatc acacagtcgc gcagccagtt gagagcagac
     7921 cgaagcggag ttcgccattc ctgagtccag ccgggacggc cgccggagtt gcacccgcag
     7981 ttgctgcgcc agcgctccac gccgtgcagg caactccagg aggtgtcctc aaaaatttcc
     8041 acctcgtgcg taggtggatg tctttccagg tactccccat agttggtgag ccgggccagg
     8101 cctttcgctt ctatatggtg gagcgcgtag gcaagggcca tctcgccgtg gtagtgatga
     8161 tgcccgtagc tctcgccatc ggtcgcgata tgggcaagct gcggccaggt tctggcgtcc
     8221 gaatacacgc tcatgaggcg ccccgcaaag tcctctccgt tgtttaacag cctttcgaac
     8281 gcgacagcgc gggagacagg gccatcgtag aagaacaatg cgatagtgct attgtccggc
     8341 agacgcaacc ggtaagccat ggtgggatca atccgcgcgt cgtggacgtc gtgccacgaa
     8401 cggccgccga tcttcctgat ccgccgtgcc tgatgcggag ccagaatggt gaacttgatg
     8461 ccatgtttcg ccatcaggcg cagcgattcc aggtccaccg ccgtttcggg aagccacatc
     8521 ccttcgggcg gacgatggaa ccggtgctcg aaatcgcgaa tgccccatac gatctgagtt
     8581 tccttgtcgc ggcagttgga cagcggcagg atcatgtggt tgtacgcctg agcgagtgcg
     8641 gagccgtggc cggaaaatct ctcgcggctg gcacggtcgg cctctacgat atccgcaagc
     8701 agatccggag cctgggactc gatccaggag agcagcgtag ggccaaagtt gaagctgatg
     8761 cgggagtagt tgttctcgat ccggacaatg cggccctcgc catccaggat tcgggcagcc
     8821 gcgttcggag cgtagcattc cgcgctaata cgctcattcc aatcgtgata agggtaggct
     8881 gagtcctgta attcaatagc ttccagccag ggattttctc gaggcggctg gtagaaatga
     8941 ctgtgaatgc agatatatct catcgacagg gagtcgttat ccctcccaca tgcggagtgg
     9001 acgacatttt taaagcttaa ctgaatcgca acagcagccg tttcgtgttg aggcctggac
     9061 gggtgaaatt gcaagaatcc cgccggaccg ctagacttga aagagaaatc atctgttagc
     9121 caaggaattt ccgcttagcg aggacacgat gtcagcagcg cgagacgtca gccgaagaga
     9181 cgcagtcaaa cttgcgggag ccgccacggt cgcggctgct gcctcgacca ttcagggcgc
     9241 tcccttcatt tcgaaggtga aagcggccaa caaccaggtg ccatatgcgt tcatcggcac
     9301 gggaggccgc ggccaatacc tgctcaagca cttcaacgga atcgattccg ggcggtgcgt
     9361 tgcggtatgc gacatttacg agcccaatct caagaaaggc atcgagacgt cgaaggacaa
     9421 gccccagggc tacttcgatt accgcgagtt gctggcgcgc aaagacatcg aggcggtctt
     9481 tatcaccacg ccgttgtaca tgcacttccc cgttaccagg gatgcgcttt tggcaggcaa
     9541 gcacgttttc tgcgaaaaga gccttgtctt ccggccggag gaaattcacg ccttgcgcaa
     9601 actggtaaac gagcggccga agcaggtgct gcaggtaggt ttgcagcgcc gctacagcaa
     9661 gttttaccaa accgcaaagc agatgatcga caagggcgtg attggcaatg tgacacacgc
     9721 ctatgcgcaa tggcatcgca atccaggctg gacgatgaag gagttcccga cgcgccagcg
     9781 cgagatgaac tggcggttgt ttcgagagtt ctccggcggc ttgaccgccg aactggcttc
     9841 gcaccagatc gacgttgcag actggtgttt cggagccaca cccgagttcg ttgtgggcgt
     9901 tggcggcctg gagtatgtga aagacggacg cgacgtttac gacaacatcc agcttattta
     9961 taagtatccg cgcggccaga agctgattta tagctcaatc agcacgaaca aacatctgtc
    10021 gttgtttcag ggaaccagaa ccgagttcgg tgagcgcatc atgggtaccg aaggcaccat
    10081 cgaaatcacc gttggaacag ataatgagcc tgccatcggt ctttggtaca gcgaacccgg
    10141 accaaaggtg accaaggaag agcagaaaaa gaaggtggag gcggttgccg gcgccacatt
    10201 ggccagcacc ggcagcggag cccgggcctt gccgatcctg ttcgagcgtg acatggtaac
    10261 cggcaaagag tcgttcatcg aacgggaatt gaagttcgcc cggcgttggc tctacgccaa
    10321 aggcatcatg gttccggaag agaaccggaa tccggtggat attgaattag aagaattcct
    10381 cgaatgtgtg cgcaccggcg ccagacccaa ggcggatctc gaaatcggtc tcgcggactc
    10441 gactgcggtc atattgtcca atctggcgat ggacgaaggc cgtcgggtct atttcaatga
    10501 gatcgacaaa atgggtctat ctcagcctgc cgccgccaac agccataaaa cttagtacac
    10561 aggttttgaa tagaccgctc cctcgcggtc gcggctcgga ctgagtcgcg cgcgagtaag
    10621 cggttatggc tgccggactt ccggtagcca tacaatcgtc agtatgtcca cgtaccgcga
    10681 ccgtcgcgcc gcatccattg aaaacaacgc tgttcgagtg accgtgctgg tagaaggtgg
    10741 gcacatcgcg gaaatctgcc ataagcagag cggtgtgaat cctctctgga cgcctccctg
    10801 gccgtcgatt gagccttcca cttacacgct ggaaaaacat cccgaatatg gtgcggactc
    10861 cgagagcaag cttctggccg gcattatggg acacaacctc tgcctcgata tctttgggcc
    10921 gccctcggct gaggaagcgg cggcaggact caccgtccat ggcgaagcct ccgttgttcc
    10981 ttaccgcatc tcgaccgaag ggcggacgat gacggcacgg gcggatctcc ctctcgcgca
    11041 attacagttt gaacgaagaa tcacggtgcc ggaggctgat ggtatcgtcc gtgtcgagga
    11101 gacagtagag aatctctccg ccgcggaccg gccgatagcg tggacgcaac atgtaaccat
    11161 cggaccgcct tttctccaga agggatccag ccagttccgg gcttccgcta cgcggtcgcg
    11221 cgtgcatgag gaagacctgg aagggagccg gctcaaaccc ggcgccgact tcgattggcc
    11281 gatggcgcca cggaaggacg aagccgcgta cgatttgcag acttatacgg atgcgccgga
    11341 atcgagcgag tttaccacgc acctgatgga cccaacccgg gaacacgcat ttttcatggc
    11401 atggtcgccc gtttcccatg tgctgttcgg ctatctatgg aaatgcgcag acttcccctg
    11461 gcttggtatc tgggaggaga actacagcag gaagcagccg ccatggagcg gaaaaacact
    11521 cacccgcggc ttggagttcg gggcttcacc tatggcggaa acgcggcgtg aaatgataga
    11581 ccgaaacaag cttttcgggg ttccggcata ccgctggctg ccctccaaag cgaagctgcg
    11641 tgccggatac tgcattttca ttctcacgcg cgaccgaatt cccgagcggg taagctggaa
    11701 cggcacagac agcgtagagt tcgaataacg gctgctcccc gccgtcagga ggtgtgcgta
    11761 tgcgactcct ctgttgttac agcgaaatcg ttatcttcct ctggcgcaat ctcgagagcg
    11821 gcgccgccac cggccgcctt ttcctctctg cgctgcagcc gcttttccat ttttagctgc
    11881 tgccgttcct tccgctgttg ttccttctgg cgtttcttga atgattgtgg cccacgacca
    11941 gccataactc ctcctggata agatataccg ccagccgcct cgcgcggcgg cccgattggc
    12001 gaagcgttct gccgcaacct tcttccttac cgctccaaac gggatgagta gcgggggcgc
    12061 ggctgtataa cgctcgtcaa ataatcaggc gggcaaggac atcttcagta tagcagggtc
    12121 gcctccagtg agttaaaagg cctatttggc tgccactttg caccagccgg aacatccaaa
    12181 cgaagtaatg gcatcgcctc acactcaaga aatttcgtac tctatcctgg acctcgcccc
    12241 ggtgagggcc gggggtagca tcgccgaatc cttccggaac atgctggatc tggcgcgtca
    12301 tgccgagatc tggggatata cgcggttctg gttggcggag caccacaata tgccgggcat
    12361 tgccagcgcc gcaacctccg tgctgatcgg ctatgtcgcc ggccggacct cgaccctgcg
    12421 tgtgggctcc ggaggagtta tgctcccgaa ccatgcgccg cttgtaattg cggaacagtt
    12481 cggaaccctc gagactctct atccgggacg catcgatctt ggcgtcggcc gggcgccggg
    12541 tacggatggc ctgaccgcta gagcgctgcg ccgtggcttg aatggcggtg aggatgattt
    12601 tccgaagcag gtagcggaac tgctggcata tcttgcgccg gcgggtcccg gccagaagct
    12661 gcaggctgtg ccgggcgcgg ggacgaacgt tcccgtttgg ctgttgggtt cgagcaccta
    12721 tagcgcccaa ttggctgcca tgatggggtt gccttttggg tttgcgagcc acttcgcgcc
    12781 ggaattgctg tttcaggcta ttcagatcta tcgtgaacgc ttcaagccgt cgccgttcct
    12841 ggagaagccc tatgtgatgg ccggcgtgcc tgtggttgcg gcagatacgg atgccgaggc
    12901 gaaccgcctg gcaacctcac cggccctccg gttcctgaaa ttgctgcgca acgaatcgct
    12961 ttttatccct ccgcccgtgg cctcgatgga tggactttgg agtgatccgg aaaggcagat
    13021 tgtggaatcg aagctccgga cctccgttgt gggtggtccg gaaacggtcc ggcgcaaact
    13081 ggcaaggttt ctcgaggaca cgcaggccga tgagctcatc ttcacgtccg acgtgtacga
    13141 ccctgcggca cgtctgcgat cttttgaaat cgccgcggca gcgatgaaag gcattcgaga
    13201 cggctgcgct aaaatgaaat aagggcgatg gagttcgcca taaccgcctt tcgagggctg
    13261 atgactccta caaacttccg tataggagtc aacgtttgcc tttacaaacc attttgtgga
    13321 tttcgttagg gtcgattgtg gggggcaacc tccggtactt tgttgcccgt ctgaccgcta
    13381 aactcattcc ctccgatttt ccgtacggca cgctgctggt caacgtcacc gggagcctga
    13441 tgctcggatt cttcctcgtc tggacaacgg agcgcgtgtt cgccgatccg cggtggcgat
    13501 tgcttatcgc cgtcgggttt tgcggtgggt atacgacgta ttcgggcttt gcgtacgaga
    13561 cctttgccct gttcgagcag ggccagtggg cctcgtcggc ggtgaatctg ctgctgacca
    13621 acgttctctg catgtttgct gttattctcg gtgccatgct ggcgcgaagc ttataggagc
    13681 cgactgatga acaccgctgt gcaagtcacc atttacctca acgagtacga ccagtggcac
    13741 cggaagccgt tacacgccga gatccttcga atgctgcgcg aagaaaacgt tgccggtgcc
    13801 tgcgcttttc actccatcgc cggcttcaac ggccggaata gcgtccacac gaccagcctg
    13861 gtggagaccg gaggaaatgt gccggtcata gtaattttcg tggacatgga ggaacatgtg
    13921 agcagagtcc ttccacgact gcgcgaaatg gctccgcacc gtctcatcgt ccgcgagaac
    13981 gtcgtcatcg agcaaggcaa cctggactga gccggctagc cgcgcaagta cttgttgaag
    14041 aaatcgagcg ttcgctgcca ggccagtttt gccgcggcct cgtcgtaacg gggcgtggtg
    14101 tcgttgtgga aaccgtgatt cgtgcccgca tagaagtgcg cggtgtaggt agcgtggttc
    14161 gctttcagcg cctcctcata cgcgggccag cccgcgttga cgcgagtgtc gagcgaggcg
    14221 tagtgaagca gcagcggtgc cttgatacca ggcacctcgt ccgctttggg ctgcccgcca
    14281 tagaatggca cggccgccga aagatccgag cccaggcgca cggcgagcat attcgagatc
    14341 gctccaccaa agcagaatcc gactacgccg attttaccgg tgcaatcgga acgggacttg
    14401 agccagtgtg ccgcggcgat aaaatcttcg ctcattttcg cgcgatctac ctggcggaat
    14461 agctgtccgc ctttctcgtc gtctccggga tagcctccaa cgctggtgag cccgtcgggc
    14521 gcgaaagcga cgaagttctc cgtgcccagg cgccgcgcca catcctcgat gtagggattg
    14581 aggccacgat tctcgtgaat cacgaggact ccgggcagct tgccagcctt ggcgggacga
    14641 accagataac cccgaatcga cccattgcct ttgggggaat ccaccgtttc gtacgcagtc
    14701 cggatgcgtg ggtcctgctt ggcgacctgt tcagcccagg cgtagttcgg gcgaaggctc
    14761 tcccagatgg cggccgctgt gagaccaccc gaggcgaact ttttcgcacc gtcgagaaaa
    14821 ccgcgacggt caatatcccc atgcacgtag cggtcaaaaa gatccaacag ctcctgcgga
    14881 aagtctgaag ccttttttcg attcatgtga agtgcccctt gtgggctgag aatagcacaa
    14941 gagcgggcgt gcggagatac ccgcgtctaa gggcttgttg aaaaacgcag aaaaccgctt
    15001 gctggcgcgc gcggctcggt ttcagagccg cgaccgcgag ggagcgggac ctgcaatgcg
    15061 ttctttccac aagcgtctaa gggcctttat cccagcaggt ccaatgcgcc ctggagttca
    15121 ctgcgggtgt ggagatctcc ggtgcgggta gtgacttcga tgcctttccg gtacatcgcg
    15181 cgggcgtctt cttcccggcc cattttttcg taggtctggc cggcatggaa gtacgccgcg
    15241 gcgtaatcgg gattcacggc caatagtttc ccgtactcct ctacggcttt ttccaggtct
    15301 ccgttgcgtg tgtactccat cgccagacca tagcgggcga aggtgtcatt ggggttctgc
    15361 gacagcagat tctgcaacgc ttccagacgg gatgtagaca ttggcgatcc agctacccat
    15421 gttaatatac cgccatggct tgcccgttct tctacccggt ttcgaagctg gactcgagcc
    15481 gccggaggac ccttccgcgc ctccctctcg gagacgcgta tgagggcatt tgccgcagcc
    15541 tgcctcagcg ggatcatgtc ccggatgagg cggcgctccg ggaattgtgt tccttcgggt
    15601 atgcgcgcgg aacctgttcg cggcaccctg ctggagaatg tcccgatgcc gtccgtatca
    15661 gtccaatgcc gggcgatggt ccattaatca aattgatata tgtgctggag cgcgctcatg
    15721 caccgctcga gcacggaacg gccgtctatt ccgcggcgga taaacgttgg atcgaggcgc
    15781 cgcagaacga aatcgtgcgc cgccaggccg aggtgtacgc ggaaggcttc gctcaacgaa
    15841 aatacggcgt gtccaatgtt ctgaaggccg cctgctgaat ttatcacacg aatggatgaa
    15901 ccaatagaag gaaagccagt gcgcgaatcc gtgtccgaat gttcggagat cgcacttccc
    15961 aacgatgcca acccgcttgg ccacctgctg ggcggtaggg ttatgcacct ggtggatctt
    16021 gccggagcca tcgccgctat gcgccactcg cggctggcgg tggttaccgc cgccatcgac
    16081 tacatgacct ttctgcatcc catccacatc ggacagttga tcaccctgcg atcttccgta
    16141 aaccgggtgt tccgcacgtc aatggaagtc ggcgtaaaag tgttcgtcga ggatctgggg
    16201 acgggtgagg tgcgccacac ttcttccgca tacctcacgt ttgtggcgat cgatgcggct
    16261 ggacaacgga ttcgcgtctg cccggtgatc cccgaaacag ccgaagaaaa gaggcgctac
    16321 gaggaggcag ggcgcaggcg gcaacacaga ttggaacaga gggaacgaag cagagtttag
    16381 atggcccggg caccgccggc gatagcggca cagagatcgc cggcgagttg ctccgttatt
    16441 cgttatcggc cttgtacatg tacttgatgt tcggcttaaa gatcaggaga ttgggctctc
    16501 catcacgatt aatcttcaag caacacttgt cgtaccactc tatgatgccg tggagggttt
    16561 ctccgtcctt aagtacgaac accatgggag ttttggcctg catctgcttg gtgtagtaaa
    16621 aattctccgc atgggtctgc tcgggaggta cagacttctt ttgactttga tttgcactgc
    16681 gacgggggtt catctgatcc ttcatgccat tcaatgaggg acgtatcaat tttcggttcg
    16741 ctgcctccac gataccactc cttgtataag tacagtcttt tggtcctgga gcacgtttct
    16801 gacttcagag ccgactcttc aggaaggaat taaacgtccg actatggttt attcatacca
    16861 tgcaagccga gcgtttatca acccgaacgg gcctttgcac gcaggtccac gacgaacgca
    16921 tcgagggcaa tggttttctg aatgttgcgc cggagaaagg ccgcgatttc gtcgatcttc
    16981 cgtgttgcga gcgagatcca gtcgaaggaa accgactggg cgataggagt gagttctttc
    17041 ttcagatcga tgttgcgaag gagggcggcc gttccagcgc cgcattcgtt tagtaccagc
    17101 aggtcctcaa tcagcagata cagaactttc agataggcat cgagcttttc cgacttgctt
    17161 gcgccgatcg tttcggaatg cctggcccac aacgcgaagg gctcgcgccc ggccgccacc
    17221 tgaagcagcg ccagcatggc cgtccggcgc ttatcgtagc tatcgagatc gatggagacg
    17281 gccgctcccg ggcagccgct cgccagcgcg atccgcctgt ctatgtggtc tatgccacgg
    17341 ttttgcaggt agacgagcat ctggtccggg ctgagcggcg ggaagtgaaa tgggaccgcg
    17401 cgggaccgta tggtcggcaa caggtcgtaa gggttggagg cggtcagtac caggaccaga
    17461 tagtccggtg gttcctccag aatcttaagc agcgaattgg cggcttgctc gttggcgcgg
    17521 tcgatctggt ccacgagaaa cacgcgccgc ttgcccttga gaggaccgta ttgtgcgcgc
    17581 tctttgagca ggcgcatctg ctgaatggag atctgccgga gcggaccatc gggcggaaac
    17641 gtcagaaagt ccgggtgggt gccgaatacc agcgggtcct gaacacgctt ttccgaagtc
    17701 caacgctccc gctcggcaac gtaggcggcg ttttcgggac ggctcaggtc gtccttctct
    17761 atctttcccg gatctccgag aaggcgggcg gcgaatcggc gcgccagcgt cgccttgcca
    17821 actccttccg ctccggaaaa gagcatggtt tgcggaatcc ggttttcgcg aatcatgagg
    17881 tcgagcgccg cggtcaccgc ctcatgacca taaaaatcgt caaacataag cgcttatgct
    17941 tatattggca aatcgcgacg ggagcgcgag tcctttcgtc aagctatcct atgtctttat
    18001 gaagttaggg gtagacttcg gcaccactcg cgtcgttgtg gctgcggcgg acagaggcaa
    18061 ctatccaatc gtaggcttcg aagcgcccga tggggctata cacgaatggt ttccacccct
    18121 ggttgcggtg cgagggtcgc agcgtctcta cgggtggact gcgtggcggg cgcaggagta
    18181 ccccggctgg accatcgtcc gttcgctcaa gcgctatctg gaccagggcg gaccgcagac
    18241 cgaggttcac atcgcggacc aaacggtctt attgggccag ttgctttccg aactgatgca
    18301 ggcgctcaaa tcggcgctgg tggatcgttc gacggccaac ctgagaacag gtgaacaggt
    18361 ggaagtcatg ctgggcgtgc cggcaaacgc caacacgaat cagcggtttc tcacttccga
    18421 agcgttccgc tgtgcggact tttccgttgc cggaatgctg aacgagcctt ccgccgcgag
    18481 tatcgagttc agtcataagc gaggcggcac gaactcgcgg atcctggtgt acgatctcgg
    18541 cggcggcacg ttcgatgcgt ccctggtgga atgcaacgga ggcagtcaca cggttgtcgc
    18601 ctcggagggt atcacgacac tgggcggcga cgactttgat acaattctgg ccgaacttgc
    18661 cctcgaatcg gcaggggtgg agcaggaaga acgcgacggc atgacacaag cggagtggtt
    18721 ccgccttcag gatgaatgcc gggataggaa agaggcgcta catccgaata cgcggcggat
    18781 tatcgtggac ctggaacgcg tgcggcagga gtgggcgccg gtgacaattc tggccgcgga
    18841 tttttacgca gcttgccgtc cgctggtgga ggagaccatc cacgccaccg aagacctcct
    18901 ggcgcggcac gccgccggga aagaaatcga cgccttgtat gtcaccggcg gagggagcga
    18961 attgccgctt gtctcgcgcg ccttgcggga acggttcggg cgccgtgtac ggcgctcggc
    19021 gcacactcgg tccgcgacgg ctatcggact ggcaatacag gcggcggaca ccgaaggtta
    19081 cgcgctcaag gaccgcttca ccaggtattt cggcgtctgg cgcgaaagcg aagacgggcg
    19141 gtctatcgtg ttcgatccgc tgttcgacaa gggcaccgga ctaccgggac caggtgagcc
    19201 gccgctggag cgcacacgtg tgtacacgcc cgtgcacaac gtgggacact tccgctacat
    19261 cgaagcgagc cactgcgacg aatccggcaa cccgcaaggc gacatcacgg aatgggacga
    19321 aattgttttt cccttcgacc gggcactgca agagcttccc gacctttcgc gagtggacgt
    19381 agagcgccgg acagcgggtg ggggcgagat ccgggagttg tatcgttgcg atagtgccgg
    19441 gtcggtatgg gtggaaatat cggacctggc gacgggacac cagcggacct tccggctggg
    19501 gcgctggctc cggtccgacg cccccgttaa gccgggacgc aagaagaaat cagccaaagg
    19561 gtaggaacga gccgatgtca aagaaaaggg atggcgtagc gagaaggcag ctcctgcagg
    19621 tgggagccgg cggtctggcc gcggcgccta tgattgtccc cgcatccgca ttgggattgg
    19681 gccaggctgt tgcgccgagc gatcgcgtga ccgtgggcgg cctgggtatc ggcgggcggg
    19741 gagcgagcgt gctgcgctcg tttctggcgc agcccgatgt acagtttcgc gcgatctgcg
    19801 atgtccggaa tgaacggcgc gaggcgatca aaagcatggc cgaccagcag tatgggaacc
    19861 acgactgcgc gatgtacagc gatcaggaag aactgtgggc ccgtaaagac atcgacgcgc
    19921 tcctcatcgc aaccggggac cgctggcacg cgccgctgtc catattggcg gcgaaagccg
    19981 gcaaggatgt ttattgcgag aagccctgtt cgatgactat cgccgagagc cgggcgctcg
    20041 cggacaccta tcagcggctg ggccggattt accaggccgg gacacagcgg cggaacggtg
    20101 cgaatttcat caaggcttat gacctggcgc ggagcggtaa gttgggcaaa ctgaccgcgc
    20161 ttcacgcgga ggcaggcccc ggcgaccggt gggctccgct gacgagccat gcctggctgc
    20221 ccgcggaacc ggagccgtcg aaacaggtgg tggattggga ccgctggctc ggtccggctc
    20281 cgtggcgtcc gttcaactcg caatatatcc gggggcgctg gcgcggctac ttcgatttcc
    20341 acggaggagg aattctggaa tggggctccc acaccgtgga cctgtgccag tgggtgggcg
    20401 atctggacga cacgacgccg gtggaattcg agccgcacgg aatgggggcg gacacgccgt
    20461 ataccatcta cggccgttac gcgaatgggc taaagctggt gctccgggac aaaggcttcc
    20521 tgggtttggg aacctgccac atccgcttcg aaggcgatgc cggatgggta gagaccgccg
    20581 atggaggcaa gatcgaagta tcggcgaatt tgcgtggcga ggacctggcg gtatcgccgc
    20641 aggaagctgg acgagccacc gccaaccatg tgcgggattt cgtcaactgc gtcaagtcca
    20701 ggaagcagcc gaagtcgaat gctctggcgg cggcgcaggc gcacattgcg agccacgcgt
    20761 cctacatcgc tttccagctt ggccggaaat tgaacttcga cccggcgacg gataccttca
    20821 cgggcggggg cagtgaggaa gcgaaccgga tgcgttcccg cgccatgcgg gaaccgtggc
    20881 ggatttgagg acgagggccg ttatgtcgag acgcaatttt ctgacgggac aaagcatgat
    20941 ggcaagcgcg gcggcgcagg gcacagcgcc tcagcagccc gccgcggcga aaccaaagcc
    21001 ggagcaacca cccgtgattc ctccaatggt ggatagcaag tatggaaccg cggagcagat
    21061 catgtcctta ccggccgcgc ggctgattgc gattctgacc gatcgggacg gatcggagta
    21121 tgcgaaggcc aaagcctgcc agcgtctggc cgtcatcgga ggcccgcaag ctgtgcccgc
    21181 actggcggcg ttgctgagcg atccgaaact ttctcactac gcgcgcttcg gtctggagcc
    21241 gaaccccgat ccatcggcgg gagcggcttt gcttggagcg ctggaccggg tacagggcag
    21301 tctgttggta ggaatcgtca actccatcgg aaaccgcaag gacaaaaggg cgatcgctgc
    21361 attggggaag ctgcggtccc acaccgattt ggaagtagcc agggccgctg atgcagcttt
    21421 ggcgaaaatc cggccggggc tgtagcgcgc gcacgatcac cggcgttcaa cgctgatata
    21481 gggagggctt catgccatcg caactctcgc gccgcgaact gctgcgagtt gccggcggtg
    21541 ccgggttttt cgcgttcgcg gacctggcgc cggctgccgg aacaccgcct ggagtcctga
    21601 cgttccgcca catccatctg gactttcaca cctcgccggc ggtgaccgga gtgggttcgg
    21661 agttcaaggc cgaagagttt gcgcgaatcc tgaaagaagc cgctgtcaac tccattacga
    21721 tttttggaaa gtgccatcac ggcatgtcgt actacccgac gaaagtgggt acgcggcatc
    21781 ccgagctgaa agtcgacctg gtaggggaga tgatcgaggc gtgccaccgc cagggcattc
    21841 gcgttccggt ctacatcagc acgatgtacg accagcatat ctggcgccgg catgcggact
    21901 ggcgtgtgta tgacgaagaa ggcaaaccgt tcgggctgcg gggtccggcg ggtccgttga
    21961 aagcggagct aggccgtgtg tgcgtgaaca cgccgtacat ggattacgtc ctggctcaag
    22021 ccgaagaggt gttaacgaac tacgaagcgg acgggctgtt ctacgacaat ttcgcctatc
    22081 cggcgtttgg ctgcctggga ccgtcctgca tggccgaacg cgaaaaactg gggctcgaat
    22141 cggctcgcct ggaagaccgg cagaagcatg cgggcatggt cacggagcgc tccattgcga
    22201 ggtttgcggc cacggtccgc gcgcgcaagc cgaa
//
LOCUS       DASTMK010000511         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_153423, whole genome shotgun sequence.
ACCESSION   DASTMK010000511 DASTMK010000000
VERSION     DASTMK010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153423"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VFQ79_21195"
     CDS             <1..796
                     /locus_tag="VFQ79_21195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0142251.1"
                     /translation="EPLPPVDYLIMESTYGDRLHKENLAVSAKLADIVNRTCARGGKI
                     VAPAFAVGRTQQLVLLLHELINAGSIPAIPMFVDSPLAVNVTEVFRQHPECYDDHART
                     YLMNGEDPFGFHRLRYIRDVAESKALNDLHGPFLIISASGMCEGGRILHHLRNNIADP
                     RNTVLITGYMAENTLGRKIVEKWPEVPIFGEPIRLRAEVAKLNELSAHADQRELLHWM
                     KPMAGSLKKVFLVHGEPLQSSALARIIQQEFNIEVVVPRREDVFQL"
     gene            895..1233
                     /locus_tag="VFQ79_21200"
     CDS             895..1233
                     /locus_tag="VFQ79_21200"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEU0142252.1"
                     /translation="MKSDLIHLAPNLAYSFAIYCYLQGLDLLTTLALLLHGYPEGNPV
                     VRFALEYAPSPLWGLAAVKLIAMLIGVYCWIAGRASALRKANILFAAAVAWNTFVVLL
                     AAVRLPTSAG"
     gene            complement(1500..1943)
                     /locus_tag="VFQ79_21205"
     CDS             complement(1500..1943)
                     /locus_tag="VFQ79_21205"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0142253.1"
                     /translation="MPQPSGAPARAKVQDRVAQNLACVDGHFHSEAENDVEAALELYT
                     DDIVFEAAALNGLDRLFSGKQAVAKFYRELWTTMKDIQFQFLQRFATEDRVVDDSLVT
                     FEVVRDGVWPHFSAGQKIEMRLVHIFEMREGKISKEIVFDMGRPI"
     gene            complement(2027..2203)
                     /locus_tag="VFQ79_21210"
     CDS             complement(2027..2203)
                     /locus_tag="VFQ79_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142254.1"
                     /translation="MHRFAVRLISPAAVQRSVRATILDIIFATVNKTAYAEFVNAARQ
                     ARSIDQAMDLLVDS"
     gene            complement(2179..2967)
                     /locus_tag="VFQ79_21215"
     CDS             complement(2179..2967)
                     /locus_tag="VFQ79_21215"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU0142255.1"
                     /translation="MFSLRLECGDAAKDLLTAMLWEAGTAGITEHEFPGGRWELEAFF
                     DKEFDLSPFAPYHPRWKQAVSTDWVEVSRAMWEPLPVGERFYLVPEWREDAAPAGRVR
                     LTVHPGLACGTGYHAATQLALCAMERHLVAGSSFLDVGTGSGILSEAALLLGAGRVTA
                     CDIYYEAARIARRNLKQKTDVSLFCGSVRSVRTGAFETVAANLNAQSLLALMADLERV
                     CSGRLILSGFTERDVRTVQAAIHSISVSDLLEQEEWRCIVLLSG"
     gene            complement(2978..>3329)
                     /locus_tag="VFQ79_21220"
     CDS             complement(2978..>3329)
                     /locus_tag="VFQ79_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEU0142256.1"
                     /translation="IVDRKKEIIISGGENISSIEIEKAVTAHPDVLECAVVAAPDERW
                     GEVPAAIVVTRSGSGLTIEALLAFLEKRIARFKLPKVIEFSTEPLPKSGTGKVRKLLL
                     KEKYWAGKTTRVQG"
BASE COUNT          679 a          988 c          922 g          740 t
ORIGIN      
        1 ggaacctctg ccgccggtgg actacctcat tatggaaagc acctacggag accggctgca
       61 taaggagaat ctcgcggttt ctgccaagct cgcggacatc gtcaatcgca cctgcgcgcg
      121 cggcggcaag atcgtcgctc cggcatttgc ggtgggacgg acacagcagc tcgttctgct
      181 gctgcacgaa ctgatcaacg ccggcagtat tccggcgatc ccgatgtttg tggatagtcc
      241 gcttgcagtg aacgtgacgg aggtgttccg ccagcatccc gaatgttacg acgatcacgc
      301 gcgcacgtac ctgatgaatg gcgaggaccc gttcggattc caccgcttgc gttatatccg
      361 cgacgttgcg gagtccaagg cgttgaatga cctgcacggg ccgttcctca tcatctccgc
      421 gtcgggcatg tgtgaagggg ggcgcattct gcaccacctg cgtaataaca tcgcggaccc
      481 acgaaatacg gtactgatta ccggctacat ggcggagaat acgctcggcc gcaaaatcgt
      541 ggagaagtgg ccggaggtgc ccatctttgg cgaacccatc cggctgcgcg ccgaagtggc
      601 gaagttgaat gagttgagcg cccatgccga tcagcgcgag ttgcttcatt ggatgaagcc
      661 gatggcgggt tccctgaaga aagtgtttct ggtgcacggc gagccgcttc aatcgtccgc
      721 tctagccaga atcattcagc aggaattcaa catcgaggtt gtagtgcccc ggcgggagga
      781 cgtttttcaa ctgtaacggc gtctgggtac cggtacaggg tccatctccg gatgggacgt
      841 ttgggctttc cggttcatcg atcgctcgcg ccgaccgacg tagtatggat gcggatgaaa
      901 tcggatctca ttcatcttgc gccgaatctc gcctactcgt tcgcaattta ttgctattta
      961 cagggacttg atctgctaac caccctggcc ctactcttgc atggatatcc cgaaggcaac
     1021 ccggtagtcc gctttgctct cgagtatgcc ccttccccgc tatggggatt ggccgcagtg
     1081 aagttgatag cgatgctgat cggcgtttac tgctggattg ccgggcgcgc cagcgcactg
     1141 cgcaaagcga acatcctgtt tgccgccgcc gtcgcctgga acacgtttgt cgttcttctg
     1201 gccgcggtgc gattgcccac gagtgccggc tagtaactcg tggtaaagac cactccgtta
     1261 ccgtcgtggc tctgtggcac aaaccaagcc tgttcgtgcc gcctgctgtg aatcggttac
     1321 taaacctcgg cgacattcgg cagttgtatc gctctgcaag ttcgaaagca ctttcgcgca
     1381 taggggattt ctcgctcatg taggttgctt ccgagttgcc gacaatcctc tcggaatgct
     1441 caggtcatcg cccgcttggc agtttggcgt gatacgtcaa agtgggctct tctcagttgt
     1501 tatatggggc gccccatatc aaagacgatt tccttcgaaa tcttgccttc tcgcatctca
     1561 aagatgtgaa ccagacgcat ttcaatcttc tgcccggcgg agaagtgcgg ccagacacca
     1621 tcgcgcacca cctcgaaggt cacgagacta tcatccacga cacgatcttc tgtggcgaat
     1681 cgttggagga attgaaactg tatatccttc atcgtggtcc aaagctcacg atagaacttg
     1741 gctacagcct gctttcctga aaaaagccga tcgaggccat tgagagcagc agcttcgaaa
     1801 acaatgtcat ctgtatagag ctcaagcgct gcttcgacat cattctctgc ttcgctatga
     1861 aaatgaccat ctacacacgc caggttctgg gccacccgat cttgcacttt cgcgcgagcg
     1921 ggggcacccg atggttgtgg catccctcac cctccttctt aggcaatggc agtagtatag
     1981 gggaaagcgg cattcgacgc aagaaacgtg cggcgtttgt acgttcttag ctgtcaacca
     2041 gcaggtccat ggcctgatcg atggatctcg cctgacgcgc ggcattaaca aactcagcat
     2101 aggccgtttt gttcaccgtg gcgaaaataa tgtcgaggat ggtggcccgc acgctgcgct
     2161 gtacggcggc cggacttatc agccggacag caaaacgatg catcgccact cctcctgttc
     2221 gagcaggtct gaaacggaaa ttgagtggat cgcggcttgc accgttctca catcccgctc
     2281 tgtgaagccg gaaagaatca gcctaccgct gcatacgcgc tccaggtccg ccatcaacgc
     2341 cagcaacgat tgtgcattga ggttggcggc caccgtttcg aaggccccgg tcctcaccga
     2401 tcgtaccgat ccgcaaaaca aggagacatc cgtcttctgc ttgaggttgc ggcgcgcgat
     2461 ccgcgcggct tcgtagtaga tatcgcaagc ggtcacgcgg cccgctccga gcaacagggc
     2521 ggcttcactg aggattccgg atcccgtgcc cacatccaga aaagaggagc ccgcgaccag
     2581 gtggcgttcc atggcgcaaa gcgccaattg tgtggctgca tgataccccg taccgcatgc
     2641 caggcccgga tgcacggtca gccggactct tccggcagga gcggcatcct cacgccactc
     2701 cggcaccagg tagaatcgtt cgccaaccgg cagcggttcc cacatggcac gtgatacttc
     2761 cacccaatcg gtggagacag cctgtttcca gcgcggatgg tacggcgcga agggcgacag
     2821 gtcgaactct ttatcgaaga acgcttccag ttcccagcgt cctccaggaa actcatgttc
     2881 ggtgatgccg gccgtcccgg cttcccacag catggcggtg agcaggtcct tcgcggcatc
     2941 gccgcattcc agccgtaatg aaaacatcgt tccgcgctca accctgcacg cgggtggttt
     3001 tgcccgccca gtacttttct ttcagcaaga gcttgcgcac tttcccggta ccgcttttcg
     3061 gaagaggctc cgtggagaat tcaatcacct ttggcaactt gaatcgggca atgcgtttct
     3121 ccaggaatgc cagcagggct tcgatggtca atccggatcc ggatcgtgtt accacgatag
     3181 ccgcgggcac ttctccccat cgctcgtccg gcgccgccac gacagcacat tcgagaacgt
     3241 ccggatgcgc cgtcaccgcc ttctcgattt cgatagagga aatattctcg ccgccactga
     3301 tgatgatttc tttcttgcgg tctacgatc
//
LOCUS       DASTMK010000512        11717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_507257, whole genome shotgun sequence.
ACCESSION   DASTMK010000512 DASTMK010000000
VERSION     DASTMK010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507257"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFQ79_21225"
     CDS             complement(<1..896)
                     /locus_tag="VFQ79_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0142257.1"
                     /translation="MHSHTHHQHSSSTGRYLKWSLVLTTVYVVVEIFAGVAARSLALI
                     ADAGHNITDALALGIAAFGHYLQSKPADSTKTWGYHRAGVLAAFVNAGTLVLLSAYIF
                     YESYQRLMNPEPVGEFTMIVVAATGLVLNLLIMHWMRAEKEHDLNIRAAFLHMLGDAL
                     GSVAIIIGAVAIRYTGWLQIDPILSILIGGLIIWSAWDVFKESMNILLEGLPRGVELS
                     AVASAMKQVDGVLDVHDLHIWSLGSSAHALSSHVVIADLPPSESDRTLQSINNVLAAK
                     FHINHTTIQFEHVECAVSKTGCS"
     gene            1157..1483
                     /locus_tag="VFQ79_21230"
     CDS             1157..1483
                     /locus_tag="VFQ79_21230"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0142258.1"
                     /translation="MAWTVETLNEAVDAELADLPADMRARLMRIAELIEAVGLPNVRE
                     PHVKHIRGTIWEIRLTGKTGIARALYMTVKGQRVVILRVFMKRTQKIPSHEIDLALQR
                     AKELER"
     gene            1480..1767
                     /locus_tag="VFQ79_21235"
     CDS             1480..1767
                     /locus_tag="VFQ79_21235"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0142259.1"
                     /translation="MTKLKDLHRRWSKDRDYKAEYDALDEEFQIARALIEARTRAGLS
                     QTELARRMKTSQSYVARIEGGKVRPSTDALQRFAQATGTRLRILFEPGTVR"
     gene            complement(1785..2699)
                     /locus_tag="VFQ79_21240"
     CDS             complement(1785..2699)
                     /locus_tag="VFQ79_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0142260.1"
                     /translation="MTSRHRTGRRVALLGMFVSGCLAAAKIIIGLMANSTSVFADGIE
                     SAADVVASGIVLFGITLAAKPPDENHPYGHGRFETLTGLGVGMILAVTGVAISFRSLM
                     KVDETHPAPALYAIWPLLASIAAKTILSVVKFHYARKIHSEALRADAANDSVDILSGS
                     VALVALGLTLYDPSRFLAADHYGGFLVGLIVIFLGLRVVRDTSMQLLDTMPDEETMKS
                     IREVAQSVPGALAIEKCFARKTGFQYHVDLHLEVDPALSVRESHEIATEVRVRIKEQL
                     DWVADVLVHVEPYGVETVWPPAPQKTAR"
     gene            complement(2728..4440)
                     /locus_tag="VFQ79_21245"
     CDS             complement(2728..4440)
                     /locus_tag="VFQ79_21245"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0142261.1"
                     /translation="MVSHSFALGLFLAASLTAQTADVIYFNGRIATLWEAKPRAEAVA
                     VRGGRFLAVGSNEDVLKTAGADTKKVDLKGAPVLPGIIETHTHPISAALAEKDGPVPV
                     MHSIPEIQEYIRTQAATVAADKLIFVPKVYSTRLKERRYPDRYELDAAAPGRLAMADN
                     GYASVLNSALLKKLGITRDTPQPSNGKIIKDAKGEPTGLVLGASDLLGKLRATRAPSH
                     ADRLWAIREMQKAYNAVGITSTFDRGQGPDGFRAYQELQRKGELTVRTTVTYTIGARG
                     TPEQVRRDMQQIPFVTGFGDEWLRVGPIKTIVDGGILIGTAFLREPYGSNTQIYGYVE
                     DDYRGVLAVPKENLFEMARVANELGWQMTSHTTGGGATDLLLDAYEAADREKPIKGRR
                     FTITHVNFPNEQAIARAKKLGVAFDCQPAWLHLDGPAIKDVFGPERMKHFIPLRSIID
                     GGLMVGGGSDHMIRFDARDAINPYHPFFGMWMAITRKTVDGTVLNPEQTITRQEALNM
                     WTVNGAYMGFDEKDKGSIQPGMLADFVVTTKDYFTCPEDEIKDIEAAITVVGGKVVHR
                     RAGS"
     gene            complement(4484..4993)
                     /locus_tag="VFQ79_21250"
     CDS             complement(4484..4993)
                     /locus_tag="VFQ79_21250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0142262.1"
                     /translation="MGNAADSNIRADTEPSAALLVTENSEDVRFFQRTFRSWKWQIHT
                     ARNCREAGALLRREPVAVIITEARLADGSWKDLRARVDLIPDPPLVIVTSLNADDQLW
                     AEVLNEGGYDVLAKPFEQPEVIRVVSLAWLHWRHKHQEIRRSATSASDIRTPASGVAP
                     PARLRWTSR"
     gene            5101..7245
                     /locus_tag="VFQ79_21255"
     CDS             5101..7245
                     /locus_tag="VFQ79_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0142263.1"
                     /translation="MKRLLPVLLMLAGATAAPGPGEGRDLSRTGYRGWAVYGGGPESI
                     RYSTLDQVNRDNVHRLQRAWTFDTGDAFKGSEMQCNPIVADGVLYATSPKLRVLALDA
                     ASGKLLWSFDPNEGREVTNKSRNRGVTYWADGGDRRIFVVHRHFLYALNARTGKPVLS
                     FGAEGRVDLRRNLGREAPGTSMSASTPGIVYKDLIIFGSLLSEGLPAAPGDIRAFDVR
                     SGKLRWRFHTIPHPGEFGYDTWPKEAWKYIGGANSWAGMSLDEKRGLVFVPTGSAAFD
                     FYGSNRVGDNLFANSLIALRADTGERVWHFQMVKHDVWDRDLPSPPSLVQVRRGDRLI
                     DAVAQTTKSGHVFVFERETGEPLFPIEYREFPGSDVEGEVLAKTQPIPLKPPAFARQI
                     FTRDMVTRRTPEARKAVLDQLQKVKSAGQFIPPGKEGTVIFPGFDGAAEWGGSAFDPD
                     TGLLYVNSNEMPWILRLVERPPSKGTVSGRKLYISNCASCHRADLRGTPPEFPGLVNI
                     GDKYTDSDLSALIRTGIGRMPGFAHLKNDGIRAITRYLAHGEDVAVTAESEAASPIYL
                     KYTHDGYNKFLDPDGYPAVEPPWGTLNAINLNTGEITWKIPLGEFPELAAKGLRNTGT
                     ENYGGPVVTVGGLLFIGATNHDRKFRAFDKATGKLLWETTLPASGNATPAVYEVAGKQ
                     YIVIGAGGGKSGAPSGGTYVAFALPDKAAPAK"
     gene            7295..9286
                     /locus_tag="VFQ79_21260"
     CDS             7295..9286
                     /locus_tag="VFQ79_21260"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU0142264.1"
                     /translation="MPKSTRRRFVAQSGTALAARNALLAAPAAAAAQAPPQRRLLSSR
                     FPLEKVASALVPRNQWHPFPTAADRTGWQSIPSDVRTALLADGEEHLGTGWPALPATL
                     FLEFARNGNRSNYEGVRNRRRGNLRKLVIAECVHGDGRFLDDIVNGLWTTCEETWWGV
                     PAHMGMQKAGRGLPDVGEPVVDLFAAETASLLAWTEYLLGPQLEKISPLLRERIYSEV
                     DRRILKPNFDRIDFWWMGLDPKLSRSVNNWNPWINSNWLTSVLLLERDEARRGRSVHK
                     ILLSLDRFLDSYHEDGGCDEGPGYWFRAGASLFDCLDLLDSASRGAINVYDQPLIREI
                     GRYIYRAHIFDNYFLNFADAPAKVNIAGDLVYRYGKRIEDEKMQGLGAFGASRQGVAV
                     VRSDSIGRQLPAIFNVVNLRSAAPRQPLVRDVWLPGIEVMTARVSDGSERGLYLAAKG
                     GHNAESHNHNDVGNFVVYADGQPVIIDVGVETYTAKTFSANRYEIWTMQSAYHNLPTI
                     AGVMQSPGRNFTARGVRYRTGDESVEFSLDIARAYPADAGVETWVRKLRLNRARGEVE
                     IEDRYALRKPANDIALTLMTPCTVAQGASGELTFTGQGLRSPVAIQYDASVLTPKTEE
                     IAIEDGRLRSAWGERLFRVLLVSSKPPRKAEWRLRVSQR"
     gene            9292..>11717
                     /locus_tag="VFQ79_21265"
     CDS             9292..>11717
                     /locus_tag="VFQ79_21265"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEU0142265.1"
                     /translation="MNISISSSVQRVALSFLLFVPFSRAGEIVLPAPAMERNGIVPVV
                     YRMNPQATGKGELTIRWTDVHGRVVEDRKLPVVLSDEMEIRFDLDMRRAAAMRNELEV
                     RFVFDGTDKKGRPDHREDTQKVSFVAKPPYRQWWDYIILMWQNYSRKDFELLKTMGID
                     SGQYNGKNVKPAEFLLSNDLRWYAENIATDFYAEYHRWRPDRIQNWSYLQAKELYKKD
                     RTSKEAFKRHPSLSDPVWIKRIHDRLVESARNHSPYRPVFYSLGDESGVADLASYWDF
                     DFSDHSLAEMRAWLKERYGTLPALNKQWGTDFTSWDRVVPDTTDEAMKRTDGNYSAWS
                     DHKEWMDVSFSRALKMGADAVRSVDPDAYVGIGGAQMPGWGGYDYSKIVQAVSALEPY
                     DIGNNIEIIRSLNPALAVVTVAFARGPWEKHRVWYELLHGNRGLLIWDDKNEFVTKEG
                     TPGERGLEVKPYYLELKRGIASLLMNSERRADPIAIHYSQPSMRTEWMLAQRPKGEAW
                     VNRGSATERLDSDFMRLRETWCRLIEDLGLQYNFVSYGQVEEGELMRKGYRVLVLPRS
                     TSLSETEAREIRSFVEQGGVVIADGEPGSYDAHSRKLAQSSLADLLPSKSGGIHSEKA
                     SGKGKLVLLNQTAIDYHQQRLVGKEGTIHQWANKIISGAGVQAQFEVTGGSGQPVVGV
                     ETHRFVNGGVTIVGVHSNPQLRVNELGPPEFKSNERFEKPQNVKLALPSDMQVYELRA
                     GKPLGRLKSVDIALDPYEPVLYALSAVPLPELRVAGPAAVPRGANARIGISVANPSLA
                     SMHVMHVEVV"
BASE COUNT         2426 a         3463 c         3563 g         2265 t
ORIGIN      
        1 gaacagcctg tcttcgacac ggcgcactcg acgtgctcga actgaatcgt ggtgtgattg
       61 atgtggaact tcgccgccag cacgttgttg atggactgta gagtccggtc gctctcggaa
      121 ggcggcaaat ctgcaatcac aacgtggctg ctcagggcgt gcgcggagga acccaggctc
      181 cagatatgga ggtcatggac gtcgagtacc ccatccacct gtttcatggc cgaggccacc
      241 gcactcagtt cgacgcctct gggaaggcct tcgagcagga tattcatgga ctccttgaaa
      301 acgtcccacg cactccagat gataaggccg ccaatcagaa tagacaggat cggatcgatc
      361 tggagccatc cggtgtaacg aatcgccacg gctccaatga tgatggcgac ggagccgagg
      421 gcgtcgccca gcatgtgaag gaatgctgcc cggatattga ggtcgtgctc cttctccgcg
      481 cgcatccagt gcatgatcag gagattaaga accagtccgg tcgcagccac cacgatcatg
      541 gtgaactcgc cgaccggttc cggattcatc agccgctgat agctctcgta gaagatgtag
      601 gccgacagca gtaccagcgt gcccgcattg acgaatgcgg ccagcactcc ggcgcgatgg
      661 tagccccagg ttttggtgct gtctgccggt ttgctttgca ggtagtgtcc gaaggcggcg
      721 atgcccagag ccagggcgtc ggtgatgtta tgaccggcgt ccgcgatcag cgcaaggctg
      781 cgcgcggcca cgccggcgaa gatctcaacc accacataaa ccgtggtgag gacgagcgac
      841 catttcaggt aacgcccggt ggaactggaa tgttgatggt gtgtgtggga gtgcaaaatc
      901 cccctcaaat cccagtctag cagcggtgtc acaaaggttt cagacccgcc acggcgtgcg
      961 aatgcctggc cggcaagctt ctcgcgtgga agttcttcgt tgacaccgat gccaacgtga
     1021 aatggtggac attagcggtg atgatcgcat gcgccccgca gaggacggcc gcggccagga
     1081 ctgcgtttct cacacaaaat gagccccggg gcgatcacgc aaatgagcaa taccatatca
     1141 ggtatactta agttgaatgg cttggacggt tgagacgcta aacgaggctg tcgacgccga
     1201 gttagcagat ctccccgctg acatgcgtgc tcggttaatg cgtattgccg aactgatcga
     1261 ggctgtgggt ctccccaatg tcagagagcc ccacgtcaag cacattcgcg gaacaatctg
     1321 ggagattcgt ctcaccggga agaccgggat cgcccgcgcg ctgtacatga cggttaaggg
     1381 tcagcgcgtc gtaatactgc gcgtcttcat gaaaagaacg cagaagattc ccagccatga
     1441 aatcgacctg gctcttcagc gagccaagga gttggaaaga tgacgaagct caaggacctg
     1501 caccggcgat ggagcaaaga ccgggattac aaagccgagt acgatgcgct ggacgaggaa
     1561 ttccaaatcg cccgtgctct tatcgaagct cgcacgcgag ccggtctgtc gcaaacggaa
     1621 cttgcacgcc gaatgaaaac gtcacagtcc tatgtggctc gcatcgaagg cggcaaagta
     1681 cgtccttcga cggacgcact ccaacgcttc gcgcaggcga cgggtacgcg tctccgaatc
     1741 ctgtttgagc ccgggacggt ccggtaatcg cgacgattcc ggctttaccg ggcggttttc
     1801 tgtggtgcgg gcggccagac cgtttccaca ccatacggct cgacgtgcac cagcacgtcc
     1861 gcgacccaat cgagttgttc cttgatgcgg acgcggacct ctgtggcaat ctcgtgcgat
     1921 tcgcggaccg acagcgccgg gtccacctcc aggtgcaaat caacgtgata ctggaaccct
     1981 gttttccggg cgaagcactt ctcgatagca agcgctcccg gaaccgattg ggcgacctca
     2041 cgaatgctct tcatcgtctc ctcgtccggc atcgtgtcca gcagttgcat ggatgtatcc
     2101 cgcacgacgc gcagaccgag aaaaatcaca atcaatccaa ccaggaatcc gccataatga
     2161 tcggccgcga ggaagcgcga tggatcgtac agcgtcagac ccagggcgac cagcgcgacc
     2221 gagcccgaca gaatatcgac gctgtcgttg gcggcatccg cccgcagggc ctcgctgtgg
     2281 atcttgcgcg cgtagtggaa cttcacgacg gaaagaatcg tcttcgcggc gatggaagcg
     2341 agcaaaggcc agatggcgta cagggccggc gcgggatgcg tttcatcgac tttcatcagc
     2401 gagcggaacg agatcgcgac ccccgtgact gccaggatca tgcccacgcc aagacccgtc
     2461 aacgtctcga acctgccgtg cccgtaggga tgattctcgt ccggcggctt cgcggcaagc
     2521 gtgattccga aaagcacgat gcccgacgcc actacatccg ccgcggattc gattccatcg
     2581 gcgaaaaccg aggtggagtt cgccatcagg ccgatgatga ttttcgcggc ggccaggcag
     2641 ccgctgacga acatgcccag cagggcgaca cggcgcccgg tgcgatggcg gctggtcaca
     2701 attgcaggat agcgcggcaa cgaacgttta cgagccggct ctccgatgta caaccttgcc
     2761 gcccacaacg gtgattgccg cttcgatatc ctttatctcg tcttcgggac aagtgaagta
     2821 atccttcgtg gtgaccacga aatcggcaag catgcccggt tggatggagc ccttatcctt
     2881 ctcgtcgaaa cccatatagg ctccgttgac cgtccacata ttcaacgcct cctgacgagt
     2941 gatggtttgc tccgggttca acacggttcc gtccaccgtc ttgcgcgtaa tggccatcca
     3001 cattccgaag aaggggtgat aggggttgat tgcgtcccgg gcgtcgaaac ggatcatgtg
     3061 atccgatccg ccgcctacca tcaatcctcc atcgatgatg gagcgcagcg ggatgaaatg
     3121 cttcatacgc tcgggaccga agacgtcctt gatggcggga ccgtcgagat gaagccatgc
     3181 gggctggcaa tcgaacgcca cgcccagctt cttcgcccgg gctatcgcct gttcgttggg
     3241 gaagttgacg tgcgttatgg tgaaccgccg tcctttgatc ggcttttcac gatcggcggc
     3301 ctcataggca tccagcagaa gatcggtagc cccgcctccg gtggtgtgag aggtcatctg
     3361 ccatcccagt tcattcgcca cgcgcgccat ctcaaacagg ttctccttcg gcacggccag
     3421 cacgccgcga tagtcgtcct caacgtaccc atatatctgc gtgttgctcc cataaggctc
     3481 gcgcaggaac gccgttccga tcagaattcc gccatccaca atcgttttga tcgggccgac
     3541 gcgaagccac tcgtctccaa agcccgtcac gaaggggatc tgctgcatgt cccggcgaac
     3601 ctgttccggc gtgccgcgcg ctccaatggt gtaagtaacg gtggtgcgca ccgtgagctc
     3661 gccttttcgc tggagttcct ggtacgcgcg aaaaccatcc ggcccctgtc cgcgatcgaa
     3721 cgtgctggtg atgccgacgg cgttataggc cttctgcatt tcgcgaatcg cccacaaacg
     3781 atccgcatgc gatggcgcac gagtcgcccg cagttttccc agcaggtcgg acgcgcccag
     3841 caccaggccg gtcggttcgc ccttcgcgtc tttgataatc ttgccgttcg agggttgcgg
     3901 cgtatcgcga gtgatgccga gtttcttcag tagtgccgaa ttcagcaccg acgcgtagcc
     3961 gttatcggcc atcgccagcc ggccgggcgc cgccgcgtcc agttcgtaac ggtccggata
     4021 acgccgttcc ttcaaccgcg tggagtatac tttcgggacg aaaatgagtt tatcggcggc
     4081 aaccgtggca gcttgcgttc gaatgtactc ctgaatttcg ggaatggagt gcatcaccgg
     4141 cacggggccg tccttttccg caagagcggc actaatcgga tgagtgtgtg tctcgatgat
     4201 gccgggcaga accggcgctc cctttaagtc aaccttcttc gtgtcggcac cggcggtttt
     4261 cagcacatcc tcgttcgatc cgacagccag aaaccggccg ccgcggaccg ccaccgcctc
     4321 cgcacgcggt ttcgcctccc acagcgtggc tatacggcca ttgaagtaga tgacatcggc
     4381 cgtttgcgcc gtgaggctgg ccgcgagaaa aagaccgaga gcgaaagaat gggacaccat
     4441 actcgtatct tagacggtat ggccgccccg tgcgccgttt acgttaccgg ctggtccatc
     4501 gcagacgcgc cggaggagcg acgccggagg caggagtgcg gatatcggag gcgctggttg
     4561 cggaccggcg aatttcctgg tgtttatgac gccagtgcag ccaggcgagg ctgacgaccc
     4621 ggatcacttc cggctgttcg aacggtttgg ccagcacgtc gtagccgccc tcgttcagaa
     4681 cctccgccca aagctggtcg tccgcgttga gggatgtcac gattaccagc ggtggatcgg
     4741 gaatcaggtc cacccgtgcg cgcaggtcct tccaggagcc gtccgcaaga cgcgcctcgg
     4801 tgataataac ggccacgggt tcccgtcgaa gcagagcgcc ggcctcccgg caattccgag
     4861 cggtgtgaat ctgccacttc catgaacgga aggtccgctg gaaaaacctt acatcttccg
     4921 aattctcggt caccagcagt gcggcagaag gttctgtgtc ggcgcggatg ttggaatctg
     4981 ccgcgttacc catttccggc cacctccttt tgatactgct gttaacatgg tcgccttacg
     5041 caaccaatgt gtcaagaggc tagaatgact gggaaaccta ccttgtcgct tgaaccgcaa
     5101 atgaagagat tacttcctgt cttgctgatg ttggccggcg caacggcggc acccgggccc
     5161 ggtgagggcc gcgatttatc ccgaaccgga tatcgcggct gggctgtgta tggaggcgga
     5221 ccggagagca tccgttattc gacactcgac caggtgaatc gcgataacgt tcaccggctt
     5281 caacgcgcct ggacgttcga taccggcgat gccttcaagg gctccgagat gcaatgtaat
     5341 ccaattgtcg ccgacggtgt cctgtatgcc accagcccca agctccgcgt attggcgctc
     5401 gatgcggcca gcggcaaact gttatggagc ttcgacccca acgagggccg ggaggttaca
     5461 aacaagtcac gcaaccgcgg tgtgacgtat tgggcggatg gcggggaccg gcggattttc
     5521 gtcgtccaca ggcatttcct atacgccctc aacgcacgga ccggcaaacc agtcctttcg
     5581 ttcggagcag aggggcgggt ggatctgcgg cgcaatctcg gacgggaagc gccagggaca
     5641 agcatgtcgg ccagcacgcc gggaatcgtc tacaaagacc tgatcatctt cgggagcctg
     5701 ctcagcgagg gattaccggc cgcgccgggt gatatccgtg ccttcgatgt tcgaagcggc
     5761 aagctgcgct ggcgctttca caccattccc catcctggtg aattcggata cgacacgtgg
     5821 cccaaggagg catggaagta cattggcggc gccaatagct gggccggcat gagcctggac
     5881 gagaaacgcg gccttgtgtt cgtgcccacc ggctcggcgg cattcgactt ctacggctcg
     5941 aatcgcgtgg gagacaacct gttcgcgaac tcgctcattg ctctacgggc cgataccggc
     6001 gagcgtgtgt ggcacttcca gatggtgaag cacgatgtgt gggatcgcga cctgccatcg
     6061 cctcccagcc tggtgcaggt ccggcgcggc gatcgcctga tcgacgcggt ggcacaaacc
     6121 accaagtccg ggcacgtatt cgtgttcgaa cgggaaaccg gtgaaccgct gtttcccatc
     6181 gagtatcgtg agttccccgg ctcggacgtt gagggcgagg ttctggcgaa aacacagccc
     6241 attccgctga aaccgccggc gtttgcacgc cagatcttca ccagggacat ggtgacgcgg
     6301 cggacaccgg aagcgcggaa agcggtgctg gatcagcttc aaaaggtgaa gagcgctggc
     6361 cagttcatcc cacctggtaa agagggcact gtaatttttc cgggcttcga cggcgcagcg
     6421 gaatggggcg ggtctgcttt cgatcccgat acgggtcttc tctatgtgaa ttccaacgaa
     6481 atgccgtgga tcttgaggtt ggtggaacgg cctccttcaa agggaaccgt cagtggacgc
     6541 aagctgtaca taagcaattg cgcaagctgc caccgcgcgg accttcgcgg cacgcctccg
     6601 gagtttcccg gacttgtgaa catcggcgat aaatacaccg attccgatct cagcgcactg
     6661 atccgcacgg gcatcgggcg catgccagga tttgcgcacc tgaagaacga cggcatacgg
     6721 gccattaccc gctatctggc tcacggggaa gatgtggccg tgacagcgga aagcgaggcg
     6781 gcatcgccga tttatttgaa gtacacgcac gatgggtata acaagtttct ggacccggat
     6841 ggctacccgg ccgtcgagcc cccctgggga actctgaatg caatcaatct gaacaccggc
     6901 gagatcacat ggaaaattcc gcttggagaa tttccggagc ttgccgcaaa gggcctgcgc
     6961 aataccggaa cggagaatta tggaggtcct gtcgtaaccg tcggaggctt gcttttcatc
     7021 ggcgccacca accatgaccg caaatttcgc gcgtttgata aggcgacggg caagctgctt
     7081 tgggaaacca ctctgcccgc cagcggcaac gcaacaccgg cagtttatga agtggccgga
     7141 aagcaataca tcgtgatcgg tgcgggaggt ggcaagtccg gcgcaccttc cggcggtacc
     7201 tacgtagcct tcgcactacc ggacaaagca gccccggcca agtaggtggt gtgctgcaag
     7261 cggcaccggg tacggtataa attaggacaa acctatgcca aagtccaccc ggaggcgctt
     7321 cgtcgcccaa agcgggaccg cgttggcggc tcgcaacgca ctacttgccg caccggccgc
     7381 ggctgccgcg caagctccgc cgcagcgccg cctgctcagt tcgcgcttcc cgctcgaaaa
     7441 agttgcctct gcattggtgc cgcgcaacca gtggcatccg tttcccaccg cagcggaccg
     7501 caccggatgg cagtcgattc cttcagacgt ccggactgcg ctgctggccg acggcgaaga
     7561 gcatctcgga accggttggc ctgccctgcc cgcaactctg ttcctcgaat ttgcgcgcaa
     7621 cggcaaccgt tcgaactatg agggtgttcg gaaccggcgg cgtgggaacc tgcggaaact
     7681 ggtgatcgcg gaatgcgtgc atggagacgg gaggttcctc gacgacatcg tcaatggtct
     7741 ctggaccacc tgcgaagaga cctggtgggg cgtaccggcg cacatgggca tgcagaaggc
     7801 gggccgcgga ctgccggacg ttggtgagcc cgtggtcgac ctgttcgccg cggagaccgc
     7861 cagcctgctc gcctggaccg aatacctgct gggtccgcaa ctggaaaaga tttcaccgct
     7921 gttgcgcgag cggatctaca gcgaagtcga ccggcgcatc ctgaagccga atttcgaccg
     7981 catcgatttc tggtggatgg gtctcgatcc gaagctctcc cgatccgtca acaactggaa
     8041 tccgtggatc aattccaact ggcttacgag tgtgctgctg ctggaacggg atgaagcacg
     8101 ccgcggccgc tcggtacaca agattctgtt gagcctcgac cggtttctcg attcctacca
     8161 cgaagacggc ggttgcgacg aagggcccgg gtactggttc cgcgcgggcg cctcgctgtt
     8221 cgattgcctg gatctgctgg attcggccag ccgcggagcc atcaacgtct acgatcagcc
     8281 tctgatccgt gaaatcggcc gctacattta tcgcgctcac attttcgata actatttcct
     8341 gaacttcgcg gacgctcccg ccaaggtcaa catcgcggga gacctggtat atcgatacgg
     8401 gaagcgaatc gaagatgaga agatgcaggg actcggagcg tttggcgcct ccaggcaggg
     8461 cgtggccgtt gtccgctccg acagtatcgg caggcagctt ccggccatct tcaacgtggt
     8521 gaatctgcgt tcggcggctc cgcgccagcc attggtgcgg gatgtctggc tcccaggcat
     8581 tgaggtgatg acggcgcgcg tgagcgacgg ctctgagcgc ggattgtacc tggcggcgaa
     8641 gggaggacac aacgccgaaa gccataatca caacgacgtc ggcaatttcg tcgtttacgc
     8701 cgacgggcag ccggtcatca tcgacgtggg cgtagaaacc tacacggcaa aaacattcag
     8761 cgccaatcga tacgagatct ggactatgca atcggcctac cacaacctgc ctaccattgc
     8821 cggtgtgatg caatcgccgg ggcgcaattt caccgcccgc ggcgttcgct accggaccgg
     8881 cgacgagtcg gtcgagttca gcctcgacat cgcgcgcgcc tatcccgccg atgccggcgt
     8941 ggagacctgg gtccggaagt tgcgtttgaa ccgcgcgcgc ggcgaggtcg agatcgaaga
     9001 tcggtacgca ttgcggaaac ccgcaaacga tatcgcgctc accctgatga cgccgtgcac
     9061 cgttgcgcag ggagcgtccg gcgagctgac gtttaccggc caggggcttc gctccccggt
     9121 cgcgatccaa tacgacgcga gtgtcttgac gccgaaaacc gaagagatcg ccatcgaaga
     9181 cggccggctc cgatccgcct ggggtgaacg tctcttccgc gtgctgctgg tgtccagcaa
     9241 gccgcccagg aaagcagagt ggcgcctgcg cgtgtctcaa cgataacaac catgaatatt
     9301 tctatttcct catctgtaca gcgcgtggcg ctgtcattcc tgttgttcgt cccgttttcc
     9361 cgggcgggag agatcgtact cccggctccc gccatggagc ggaacggcat cgttcctgtc
     9421 gtgtatcgaa tgaatcccca ggctaccggt aaaggagaac tgacgatacg ttggaccgac
     9481 gtgcacgggc gcgtggtaga agaccggaag ttgcccgttg tgctgtccga tgaaatggag
     9541 atccgattcg atctcgatat gcgccgggca gcggcgatgc gaaatgaact cgaggtccgt
     9601 tttgttttcg atggcacgga caagaaaggc cgtcccgatc accgcgaaga cacgcagaag
     9661 gtttccttcg tcgcaaaacc gccgtaccgg caatggtggg attacatcat cctgatgtgg
     9721 cagaactaca gccggaagga ttttgaactt ctgaaaacga tgggtatcga ttcgggccag
     9781 tataacggaa aaaacgttaa accggccgag ttcctgctga gcaacgatct gcgctggtat
     9841 gcggaaaata tcgcgacgga cttctacgcc gagtaccatc gctggcggcc ggaccgcatt
     9901 cagaactggt cctatctaca ggccaaagag ctctacaaga aagaccggac gagtaaggaa
     9961 gctttcaagc ggcaccccag cctctccgac cctgtgtgga tcaagcgtat tcacgaccgc
    10021 ctggtcgaat ccgcccgtaa ccactcgccg taccggcccg tgttctacag cctcggcgac
    10081 gaatccggag tggccgatct cgcctcttac tgggatttcg atttctccga tcattctctt
    10141 gccgaaatgc gcgcctggct caaggagcgc tacggcacgc tccctgcatt gaacaaacaa
    10201 tggggaaccg atttcacgtc gtgggatcgt gtcgtgccgg acaccacgga cgaggcgatg
    10261 aaacgtacgg atggcaacta ctccgcttgg tccgatcaca aggagtggat ggatgtctcg
    10321 ttttcccgtg ccctgaagat gggcgccgat gcggtgcggt ccgtcgaccc ggatgcttat
    10381 gtcggcatcg gcggcgcgca gatgccgggg tggggaggct acgactactc gaaaattgtg
    10441 caggcagtga gtgcgctgga accgtatgac atcggcaaca acatcgaaat catacggtcg
    10501 ttgaatccgg cgctggcggt ggtcacggtc gctttcgcac ggggtccgtg ggaaaagcat
    10561 cgcgtgtggt acgagctgct gcacggcaat cgtggcctgc tgatctggga cgacaagaac
    10621 gagttcgtga cgaaggaagg aacacccgga gagcgcggcc tcgaagtgaa gccctactac
    10681 cttgaactga agcgtggaat cgcctcgctg ctgatgaaca gcgaacgccg tgcggacccg
    10741 atcgcgattc attattcgca gcccagcatg cggacggaat ggatgctggc acagcggccc
    10801 aaaggagaag catgggtgaa ccgcgggtcg gcgaccgaaa ggctggacag cgacttcatg
    10861 cgcctgcgcg aaacctggtg caggctgatt gaggatctcg gccttcaata caacttcgtc
    10921 tcctacgggc aagtggaaga gggtgagctg atgcgaaaag gttaccgcgt gctggtgctg
    10981 ccccgctcca catcgctgtc ggaaaccgag gcccgcgaga ttcgctcgtt cgtcgagcag
    11041 ggaggcgtgg tgatcgccga cggagaaccg ggctcctacg atgcgcacag ccgcaagctt
    11101 gcgcaatcga gcctggcgga tctgctgcct tcaaagagcg gcggcatcca ttcggagaaa
    11161 gcttccggaa aaggcaagct ggttctcctc aaccaaacgg cgatcgatta tcatcagcag
    11221 cgcctggtgg gcaaggaagg caccatccat cagtgggcga acaagatcat ttcgggtgcc
    11281 ggtgttcagg ctcaattcga agtaaccggc ggctcgggtc aaccggtggt tggcgtggaa
    11341 acgcaccgct tcgtcaacgg tggcgtgacg attgtgggcg tgcattcgaa cccacagctt
    11401 cgcgttaacg aacttggccc gccggagttc aagtcgaacg aacgttttga gaaaccccaa
    11461 aacgtgaagc tggcgcttcc atccgacatg caggtttacg agctgcgcgc cggcaagccg
    11521 ctcgggcggc tgaaaagcgt cgacatagcg ctcgatcctt atgagccggt attgtacgcc
    11581 ttgtccgcgg tgcccctgcc cgaattgcgg gtcgcggggc cggcagcggt tccgcgcggt
    11641 gcaaatgcgc gcatcggcat cagcgtggcg aacccgagcc tggcctcgat gcacgtgatg
    11701 catgtagagg tcgtcaa
//
LOCUS       DASTMK010000513         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_473144, whole genome shotgun sequence.
ACCESSION   DASTMK010000513 DASTMK010000000
VERSION     DASTMK010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473144"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFQ79_21270"
     CDS             complement(<1..467)
                     /locus_tag="VFQ79_21270"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0142266.1"
                     /translation="MEQKQRLFREPEQGGSFRSVDHALEVLCAFAGKEELGISELARR
                     LNLQKSGVHRIVRALAAKGFVQQLANRRYSLGFRVLELGNMYRLRLDLARNGESILRP
                     LSQRMNANAHLARIENFEVVDLLRVEYPTPLRVSRSPLLRRPAHCTALGKVLLA"
     gene            643..1689
                     /locus_tag="VFQ79_21275"
     CDS             643..1689
                     /locus_tag="VFQ79_21275"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0142267.1"
                     /translation="MDDRHEGFPDLVRLNNGELLVVYRESDAHSARTFCNLVLRRSRD
                     NGKSWSDRQVLTGEKLQNGVINKWNCPRIGYAGDGRLCLVCDRIAYPPGESGDFKHGR
                     VFLWWSSDEGRTWTGPEETPVFGIVPGKLTELSSGTWLLSTHFHNLTRSKREQIVHRS
                     TDGGKTWSDAITICGHERYNTNEGSILELPGKRLVCYMRENSNKGWPALKSFSNDDGR
                     SWRGPFETLMGGCHRPVAGFLPDGRILVTYRYNQGGLRFPLDGGGNAGRAHNVFAALD
                     TVESATAEVLSRQSTLVRPVAYDRSPRADTGYTGWASLPDGRVFMVTYMVDDAPTAHI
                     RGYWFSIRDFFLPA"
     gene            1667..3916
                     /locus_tag="VFQ79_21280"
     CDS             1667..3916
                     /locus_tag="VFQ79_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142268.1"
                     /translation="MISSFRLSLCLLALPSVAAAAAAAPCTVDRSAWPDDHVVDTAVR
                     RLCKSGAAYVRLVRLASFRADIGEEGYAFAGAARSPEIIANSARGILYGAYAFAEHLE
                     RHGRMPKPRVSKPYFRYREWQTAALQGNFNLPLGGGFDRPLEEISEVVRRTIHEAPRY
                     GINALQLMGRVGEGIDVSWFLEYESFPKLRARRPIGWSIDRRIDELRRLADEAHRHGL
                     DFLIWDHEIAFPPGFVEAYPEVRGVDYPICFSHPFILEFVDAKFDEFFRKLPEVDGID
                     LTFAETRGYNLLEHSGCKCEKCSRTSAEQKLRQLLIRVYEACRRHGKRMEVRSYNQSP
                     RHAAIMAKVLAGLPPDLVIVTKNSIVDFRGIGYPDDPMLGAFPGQPQTLELTATPEGS
                     GYGYIPALLPDFYKEKMGRAIGRKLAGIAIRTDYHLQYGHATFFTSGPPVLTFDTPND
                     FNVYVASRLAWDPSLEVEQLWKEWASARYGKDAPRAITALKRNASISEGIFFVRGFSL
                     LTHLNMVPHLATVDEELKNSYLLQFFPEHEGYRRTYNKLADPSEATIEEVLSEKQRAI
                     DEARLARSAASGIAALDQWLRKSENAARLWKQIAAVYFRLRQQPMAGEKLDNALDSLL
                     DKAYRIEREDGRVWPVFPAARGVPVYEFARQALERGKNADDAPGLWVQLIKALRPGAP
                     EFYRTFEIPALASAAEISFSSRHLHVNGIKLPLGRAVTGTNWKATRPAQVTVIPREDK
                     YEIQIRDKP"
BASE COUNT          818 a         1147 c         1198 g          834 t
ORIGIN      
        1 gccaaaagga ctttgcccag cgccgtacag tgtgccggac gcctcagcag gggcgagcgc
       61 gacacgcgaa gcggagtcgg atactccacc cgcagcagat ccacaacctc gaaattctcg
      121 atgcgcgcca agtgtgcatt agcgttcata cgctggctga gcgggcgcaa gatcgattcg
      181 ccattgcgag ccaggtcaag acgaagccgg tacatgttcc cgagctccag tacgcggaag
      241 ccgagagagt agcgcctgtt cgctagctgc tgaacgaagc ctttcgccgc gagcgcacga
      301 acaatccggt gtacgccgct cttttgcaga ttcagccgcc gggcgagctc ggagatcccc
      361 agctcttcct ttccggcaaa cgcacaaaga acctcgagtg catggtcgac gctgcggaac
      421 gatccgccct gttctggctc ccggaacaat cgctgttttt gttccactgt gcagaacagt
      481 ttatcatcgc gcgttgaatt tggttttgtc gtcgccatat cgtgaagaac caggcagaat
      541 aggcaactaa tggaacgacg ttcatttctc ggcctcgttg cggcagttgc tgccgctcgt
      601 ggctcttcaa catcgtgtgt agaaagtgtc gtcatctcac gtatggacga ccggcatgaa
      661 gggtttccgg atcttgtacg cctgaacaac ggcgaactgc tggtcgtcta tcgggagagc
      721 gacgcccaca gcgcacggac gttctgcaac ctggtgcttc gacgcagccg ggataacggc
      781 aaatcgtgga gtgaccgcca ggttctcacc ggtgaaaagt tgcagaacgg cgtcatcaac
      841 aaatggaatt gtccgcgaat cggctatgcc ggggatggcc ggctctgtct cgtatgcgat
      901 cgaatcgcgt acccgcccgg agagagcgga gatttcaaac acgggcgtgt gttcctctgg
      961 tggagtagcg acgaagggcg cacctggacg ggaccggaag agactcctgt tttcggtatc
     1021 gttccaggca agctgacgga attgagctcg ggcacgtggc tgttgagtac acattttcat
     1081 aacctcacgc gaagtaaacg cgagcagatt gtgcaccgct ccaccgacgg tggaaaaacc
     1141 tggagtgatg ccattacgat ctgcggccat gagcgctata acacgaacga aggttcaata
     1201 ctcgagctgc ctgggaagag gctcgtgtgt tacatgcgcg agaactcgaa taaggggtgg
     1261 ccggcgctca agagcttttc taacgacgat ggccgctcgt ggcgaggacc tttcgagacc
     1321 cttatgggcg gctgtcaccg ccctgtcgcg ggattcctgc ccgatggccg gatccttgtg
     1381 acgtatcgct ataatcaggg cgggttgcgc ttcccgctgg atggtggtgg aaacgcgggg
     1441 cgggcgcata atgtatttgc cgcgctcgac actgtggaaa gcgccaccgc ggaggtgtta
     1501 agccggcaat ctaccctggt gcggcccgtg gcgtatgatc gcagcccgcg cgcggacacc
     1561 ggctacaccg gctgggcaag cctgccggac gggagagtct tcatggtcac ctacatggtg
     1621 gacgacgcac caacagcaca catacgcggt tattggtttt cgatccgtga tttcttcctt
     1681 ccggcttagc ctctgcctgc ttgcgttgcc ttcggttgca gccgcagccg cggccgcgcc
     1741 ctgtaccgtg gatcgcagcg catggccgga tgaccatgtc gttgatacgg ccgtcagaag
     1801 actgtgcaaa tcgggagcgg cgtacgtccg gctggtccgg ctcgcgagct ttcgggccga
     1861 cattggcgag gagggctacg ccttcgccgg tgcggcgcgc agtcctgaga tcattgccaa
     1921 cagtgctcgc ggcattctct atggcgccta cgcgttcgcg gagcatctcg aacggcacgg
     1981 ccgcatgccc aaaccacgcg tttcgaagcc gtatttccgc taccgtgaat ggcaaaccgc
     2041 cgccctccag gggaacttca acctgccgct cggcggcggg tttgacaggc cgctcgaaga
     2101 gatctccgag gtcgtgcgcc gcaccatcca cgaggcgccg cgatacggta tcaacgctct
     2161 gcaactcatg ggtcgcgtgg gcgagggcat tgatgtcagt tggtttctcg agtacgaaag
     2221 ctttccaaag ctacgtgcac gccggcctat cggctggagc attgaccggc gcatcgatga
     2281 gttgcgccgg ttggccgatg aagcgcaccg gcatggtctg gattttctga tctgggatca
     2341 tgagatcgct tttccgccgg gctttgttga agcctatccg gaagttcgag gcgtcgatta
     2401 cccgatctgc ttctcgcatc cgttcattct tgagttcgtc gacgctaagt tcgacgagtt
     2461 cttccgcaaa ctgcccgagg tggacggtat cgatctgaca ttcgctgaaa cacgcggata
     2521 caatctgctg gaacacagcg ggtgtaagtg cgaaaagtgc agcagaactt cggcggaaca
     2581 aaaactacgg caactcctca ttcgtgtgta cgaggcgtgc cgccggcacg gcaagcggat
     2641 ggaggttcgc agctataacc agtctccccg tcacgctgcc atcatggcaa aggtgctcgc
     2701 cggccttccg ccggatctcg taattgtcac gaagaattca attgttgatt tccgtggtat
     2761 cggatacccg gacgatccaa tgctcggcgc gtttccaggt cagccccaga cgttggagtt
     2821 gacagcaacg ccggaaggca gcggatacgg atatatcccc gctcttctgc cggacttcta
     2881 caaggagaag atggggcgcg ccatcggccg caagctcgcc ggcattgcca ttcgtacgga
     2941 ctatcacctc cagtacggtc atgccacatt cttcaccagt ggaccgcccg ttctgacttt
     3001 cgataccccc aacgacttca atgtgtatgt agcgagccgg ctggcttggg atccttctct
     3061 cgaagtggag cagctctgga aggagtgggc ctcggcgcga tacggaaagg acgcgccgcg
     3121 tgcaatcact gctttgaaac ggaacgcgag catttcggaa ggaatctttt tcgtccgcgg
     3181 cttcagcctg cttacgcacc tcaatatggt tccccatctc gcgacggtcg atgaggagct
     3241 aaagaacagt tacctgcttc aattctttcc ggaacatgaa ggctacaggc gcacttacaa
     3301 caaactggct gatccgagtg aagcaacaat cgaagaagtg ttgtcggaaa agcagcgcgc
     3361 catcgatgag gcccggcttg cccggtccgc cgcttcgggt atcgctgcgc tcgatcagtg
     3421 gctccggaaa tcagagaatg cggcacggtt gtggaagcag attgcggccg tctacttccg
     3481 tctgcgccag cagccgatgg cgggagaaaa acttgataac gccctggact cgcttctcga
     3541 caaagcgtac cgcatcgagc gcgaggatgg ccgggtatgg ccggttttcc cggcggcccg
     3601 cggtgtgccg gtgtatgagt ttgcccggca ggcgcttgaa cgcggcaaga atgctgatga
     3661 cgcacccgga ctttgggtgc aactgatcaa agcgctgcgt ccgggcgcgc cggagttcta
     3721 ccggacattc gagataccgg ctttagcgag tgccgccgaa atttcgtttt cgtcaaggca
     3781 tctacacgtg aacgggatca aacttcccct gggccgcgcg gtaaccggaa cgaactggaa
     3841 agctacccgc ccggcacaag taaccgtaat tccccgtgag gataagtatg agatacagat
     3901 tcgcgataag ccttagtttc ttctccgtta ttcccatctt ctgcgccggc ggagccgtgt
     3961 ccatcgggag tgagaggcaa ctgtttgtcg acgacta
//
LOCUS       DASTMK010000514        27856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_283029, whole genome shotgun sequence.
ACCESSION   DASTMK010000514 DASTMK010000000
VERSION     DASTMK010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 27856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27856
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283029"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFQ79_21285"
     CDS             <1..533
                     /locus_tag="VFQ79_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142269.1"
                     /translation="QNLAGARLKDLRTVLAYLRTRPDVDGNAISIWGDSFAPSNSAEL
                     YLDEIEQEVSPEIQYRSEPLGALMALLAALYEDGVRAVAARGGIASYLTMLESAFTYT
                     PMDAVLLGVLKVGDIADIVAATAPKPVLFEAAVDGRNVIVDAGTLGRMFPAERRITVR
                     PERGDVAAWLIAQTKP"
     gene            complement(540..2195)
                     /locus_tag="VFQ79_21290"
     CDS             complement(540..2195)
                     /locus_tag="VFQ79_21290"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0142270.1"
                     /translation="MGTITRRAWLSSIAGGGLAFGGRIEPGLILHGGNVLTMDPAAPR
                     AQALAVAGDRIFAVGSDEDVMRLATARTRKIDLGGSTVVPGFIDAHTHVCYSGIRHLK
                     QVDCDLRSIADIQKAIRERAAKTEPGQWVVGFKYDDTKTSDGRKLNRADLDAAAPGHP
                     VYIGHRGGHTGYVNSLAMKLAGVSEDTPDPAGGHFDRDPATRRLTGGLRERATVPFQK
                     LFASRHTREEYRQGVELITRMMARSGVTSAHDALGGPESLLAYQDAREADQLAIRVYC
                     LIRNSDLHQMISAGVRTGMGDEWVRIGAVKLMCDGSISERTARLSEPYVGRPDDHGIL
                     VTDEPELYEQARRAHLAGWQIGTHANGDVAIDKTLRVYERLQREHPRKDARFRLEHCT
                     VITDDLVRRIKALGAIPNPFSTYVYYHGEKMREYGEERLNHMFAVRSFLDAGVPVTQT
                     SDYPPGPFEPMMALQSSVTRTDSHGKTWGPRQKVTVEEALRVGTVHGAYASFEERLKG
                     SLEPGKLADLVVLAADPLRTDPFKLIGIRTERTMIGGRWVYES"
     gene            2361..5828
                     /locus_tag="VFQ79_21295"
     CDS             2361..5828
                     /locus_tag="VFQ79_21295"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142271.1"
                     /translation="MTTLRMIGCSIALLSLLPAQEYRATLTGRVLDSQGAIIPNVRVL
                     ATQIATNANFETFSGSDGQYTLPFLPPGSYRILVEALGFKLFVRDGIRASTGERIGLD
                     ITLEIGSVNETITVSADRSLLETATASTGQVIRDRQIENMPMNGRTPLVLAQLAFGVV
                     PSSDPKFTRPFDNAGPSTFSMGGAPTRSNELLLDGAPNSTGNSRVAYNPPVDAVEEVK
                     VETFQADAAYGHTGGGTVNVVMKGGTNTLHGNVYEFNQVSRLAATPFFTNRAGLKKPV
                     TRYNQWGVNVGGPVVIPKLVNGRDKVFFFFAYEGIKDAFPEPLTSTVPTAEQRNGDFS
                     QLLTVGSNYQIYDPLTGVAEGARVRRMPFANNVIPAHRLSAIAKNYLQFYPMPNQPGR
                     ADGQDNFLANTVRRDDFNSELGRLDFNINDRHKLFGNFRHNEALLSRGNRFNNIATGI
                     PLRRVNWGFMVDDVYTFTPTMMLNTRAAWTRFSEANIPQGIGFDMTTLGFPSSLAASS
                     TRLVLPRVAFDRYTGLGETSGNDTPFDIFQIFSALTKVVGQHSLKAGVDLRLYRESSI
                     NFGNSSGLFTFGPDWTRGPLDNATAAPLGQDFASFLLGLPTSGGYDVNAARTNQAGYY
                     ALFLQNDFRARSNLTLNLGIRYERDLPTTERYHRSVNGFDFVTPNPISESAAAAYRLN
                     PIPELPVEQFRTPGGLLFAGPDRPEIYRTKSHYFSPRFGFAWTPAALGGKTVIRGGFG
                     VFFFGLGTTGINALGFSQTTPLVATLDGFLSPAATLDNPFPDGIRQPTGSAAGLSTFL
                     GQSVRFYNSFPQNPYSNRWNISFQRALGNNLVMEAGYVGNHTVHLAVNRQLNFVPREF
                     LSTSGVRDAAVINRMTANVVNPFAGLIPGTPLDGGTIRRSQLLGAFPHFITGDLIANR
                     TDGMPANGVILDASNDGSSYFHMLQVRLEKRFSGGLQFLANYQYSKLIEKISRLNDSD
                     LSLEKRIAAEDRPQRFVMSASYELPFGKGKPFLASEGPVLGRLAGGWILNAIFITQPG
                     PPLNWGNVIYWGGDLSLNPRRIEGAFDTTRFNTNSQQQLDWNVRTFPTRFSNLRQDGV
                     KNMDLSIIKNTAITEKISLQYRCEFFNAFNRPAFDPPNLSPTNSNFGRITNQPNLPRI
                     VQMALRLSW"
     gene            5832..6785
                     /locus_tag="VFQ79_21300"
     CDS             5832..6785
                     /locus_tag="VFQ79_21300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0142272.1"
                     /translation="MKRLAFVSAFSLLLLVALRSQEQKVFQLAPGVYFWQGDTSIRRP
                     ANCTWVLFKDYVVVIDANFPWGAREILPEIKRTTDLPIRFVFNTHYHADHTFGSGIFM
                     QAGATIVCSRECGAESRTKGEADWRNQVLNRSERPNASVHDKAVAAETREKGYRLEHP
                     TVLFDGSMAFDDGERRLELIRMGPAHSKGDAVAYLPKEKILITGDLCVNWTTGNNVGD
                     RDADPDNWIRALDKLVQWDVDIVVPGHGRPGTTETLRGQRAYLAEMVDKVRAGIQASK
                     TADQLASEIDLSHHHSFGARSSANSVRAIYRKLTEPPRSQE"
     gene            6790..8529
                     /locus_tag="VFQ79_21305"
     CDS             6790..8529
                     /locus_tag="VFQ79_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142273.1"
                     /translation="MRFPAILVCLFAPAFALAADSLPARYFRLMEAGTARVEAKLKAA
                     PDADLKTLEAESSWRHFPYAILAPAVLYSKQHRDNPRYRDKHMRDLAIRIGDMLAGES
                     EKGTFEPRLDSDWDTYMWLDAYRLLERELGDERRERWKREIRKNIAMQVEGTRERLDF
                     AWYNSPYIGTSPNHYALWAANLYLGGRLFGDKEWEDLGRTVLRRFAMVEQTPDGYWGE
                     HSRSGPTIGYNHLTLSAVAMYWEFSKDKDVLPALRRATDFHKNCTFLDGTPIDVFNDR
                     NRYWGVSAWAQFAFSNFPDGRGYAAFLTSFFDPEKLTVDQLGRLAQDALYYHEGPSEA
                     APQQRPSYSYRMSVPAGVRKTGPWQVALSGLIDTQTINNRFYLDRQGHLSIFHQKAGL
                     IITGANSKRQPELATFSEELMGQVVHMPLSSRLQMSDTQDRLSLAYNTFFSDLYVMPP
                     SDSGLDFRFVINGRGTPAKDPKLTLQLVLEPGEELETGSGRKVTVGQDKIEFGGSELG
                     GSIRHHGWTLSVDRTAQLRWPVYPHNPYSDIPETNLRRAVAVVVVPLNLKPRPGKYVR
                     PRDQEIEFKLSVP"
     gene            8535..10787
                     /locus_tag="VFQ79_21310"
     CDS             8535..10787
                     /locus_tag="VFQ79_21310"
                     /inference="COORDINATES: protein motif:HMM:NF044450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142274.1"
                     /translation="MRTLFIAAFWGAAGLYAQSLDIPITVEETAGMARKAEPVTFGVP
                     LPRGALREAAQLRLYNPEGEVIPAAFRVVNRWWDDAQTQVASIQWVHVDFFADVPAQG
                     RAVYRLRNSRAPEAPPPARLRVETVGDDIKVDTGPLQFTVHRNGPFLDAPGFGGADFL
                     LRSDERIYKAGNWKDSQLEVEEENPLRIVLKRTGSHGWVDKEDRALDYVLRIVAYAGQ
                     PQIRLTYSFINRQGRAMADFVRLDGLWLQARLDTDTPPMRIEQLQAEPRRPGWFEAGP
                     IGIGLRWFWQLYPKGFEIRSDRTLRLALFPETARPQNIYTGVAKTHEMALSFGGGNLS
                     AQLDHPLYAVAPPKWYTRDTRALGRLVESSPEAIRPEYWPLVQKYDRWLSTCRDAVLA
                     KRDRGYEFEGRRLDEYGMLNFGDALHKLIRNDRRPDYGIHWDTEYYDFPHALFLHFFR
                     TGDLKSLRTAVEAAAHLADVDISHYEAGKDCPGAARTGPGLNHWTRYSNGVFISSTSW
                     AFYKNEGLFDRYLLTGDLWSRDVARMSADWGICYNGLDLQSNTRSIGHGLFAMLKAYE
                     VFGDKKYLDRAQWIVDNVHAWQDGDVEKLRVLNPRAVWDPQFKGGYSHQSWMYGIALE
                     AMAQASQTLARPEMPGYMRRAADWIFGNPREWDPERRIFRNAPVHSVMLTPGLTYIAE
                     TSGEKKYWDVALESFTRQTEAGELTDRLKLFAQLFRNSQRFPWYLSVEGPPRFGGGTE
                     QTRRRAHGGF"
     gene            10996..12123
                     /locus_tag="VFQ79_21315"
     CDS             10996..12123
                     /locus_tag="VFQ79_21315"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU0142275.1"
                     /translation="MSLLHPLMPARVRYKLPTSWNWKMLSTFLGSGRSQVYIDVHTRL
                     DPPASYQPLVTTDSRWQLSEADIRGFWERGFIGPLKLMEPEAMRDIAPRMWRLWEAES
                     RIYPPKSYKYVGSTATGSDKTEISNEAYATKGLNARDKHLQDDELMSLYAHPAIVERM
                     AQLLGPELLLWRSQFFPKYPGMGGTGWHQATSYLNETFRTATLTPRNLRKLFQLTAWV
                     AVTDSTVRNGCMRFIPGTHWELLPMTVEEYDPIKHAGNKHDRFGTKIMRPALGDLEKR
                     AVNLEMKAGEFVIFSERTMHGALPNVTTDDARLGMSARYIVPDVKIHNPWVLGDGGLS
                     IVYLQIQKLNLDRWKIIQLRGDKKGPMADRVIPAPEGARRW"
     gene            12147..18584
                     /locus_tag="VFQ79_21320"
     CDS             12147..18584
                     /locus_tag="VFQ79_21320"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEU0142276.1"
                     /translation="MSVGESLASGMSEPIAIVGMGCRFPAGASNPEAFWRLLCNGIDG
                     ITEVPAERWDARRFYDPDPAKPGKTYVKQGGFLRERVEYFDPLAFGISPREAPALDPQ
                     QRLLLEVTWEALEDAGLRIESLAGSATGVFIGAFTLDMKMLQSNIHNRDRLTSHSTTG
                     SSMTLMANRLSYVFDWRGPSIAIDTACSSSLVAAHYACQSLWRGECTLAIAGGANTML
                     RPEYFIVMSKGHYLSPRGRCHTFDARGDGYARGEGAGVVIFKPLSAALRDRDAIYALV
                     RATGVNQDGQTAGISLPNGEAQEHLIASVYQQSCVTPGEVQYIEAHGTGTKAGDPVEA
                     RALDRALSVGRTPGHRCFVGSVKTNIGHLEAAAGVAGLIKAALCLDRRQIPPNLHFEQ
                     PNPDIPFDEMCIQVPTRIESWPAYGTRAYAGVNSFGYGGTNAHLLLEEAPVRSTQAPS
                     AELSRPLLFPISARSEQALKSLAESYAAFLNEGASLADFCHSTSLRRSHHYRRAALVA
                     ETRDELCEKLGALAAGALLPGMNVNQTLPGDACRLVFVYTGMGPQWWAMGRELMGTEA
                     VFRECVEECDDLFSSYAGWSLLDALNAPEESSRMRETQVAQPANFVLQAALTALWESW
                     GIKPDAVVGHSTGEAAAAYAAGALSLQDALLVSYHRSRLQQMQAGLGSMLAVGLSEGN
                     IADVIRPYDGRVDIAAINGRSALTLAGDTAALHQIAAALEEREVFHRFLRVDVAYHSE
                     QMDAVREPLLKALAAIQPRDTAIPFYSSVTGARTPGSELTAAYWWQNVRKPVRFAQAM
                     NTLVGDRYHVFVEAGPHPVLQNAIQEAVRAAGGAGEVIASLHRERPERATMLSALGAL
                     YTLGFSPDWSALTPPEGQYIKLPTYPWQREYYWHESGISKLDRVGQPGSVFLDTPLAL
                     PIPAWEVELNSNYFPYLADHCLDKTTIFPGAGYVEAGLALHQALRPQDVYTLEGVEFQ
                     KLLVVDPKDTAILHVRCDPNTHEFKIFSRAKHDSAAWELHALGRLMPGSVRPRPRVSP
                     AELSAKCPEEISAAGFYERLELSRFYYGPYFQTLKRIWIGPSSGFAGIQGHAALAQDP
                     APYLVHPTLLDGALQLLSLNAPLRASCPWVPRSIDRIVVYHSPASICWAYAEAVERGQ
                     GTLRGNIVLCDEDGNVTVEMTGVHCHEITTLPAQEAWQQSLYSWSWQPVAGEPALATP
                     VHTGGCLVVTDGSGGLETIITAALHAHQVPYRVTERAEPREFRTILYLCTAAEPQDAS
                     SGDFDTAARECTRLLELLHSLPAESLDAGVALSIVTRGAQSVTPEDRVTARAANSVWG
                     LGRVIRNEYPTVACQLIDLGSRPTAHDYARLVEHVLRQTPEPEIAVRGGTTFVHRLQH
                     APYNVETAEAQAVNTDEPVELVIGAPGRLDSLHYRLSQRRVPGPGEVEIRVQASALNY
                     KDILKAMGTISNKVLEGTYFGSAFGMELSGTVVATGEGVGDVRVGDKVIATTNQGSFR
                     SYVTTSATYVIPKPVSLSMQEAPAFTVFLTAYYGLMDVARLRPGERVLIHNAAGGVGL
                     AAVQIAQWLGATIYATAGSEEKRASLRAMGVPYVMDSRSILFADEIWALTAGEGVDVV
                     LNAMAGEALRKSFALLAPYGRFVEIGKKDIAENNGLPMQTFHRNVSFTAVDLDQIFRD
                     RVTLAQRLFRDVADGFDRGHFRALPTTVFPAGEAENAFRYMAQSKHTGKVMVDFSPQP
                     VNAFPVAAACSPIRHDSTYVVTGGTRGFGLEIAKWLVAEGARYLVLISRSGAAGDDIK
                     QAKAVMEGQGVRVMVAAADVAAADQVHRLWRNIQTAMPPVRGVIHGAMVLDDVLLTGL
                     TAERLRQVMAPKVLGALHLHELTKETPLDFFVMLSSVASLVGNVGQGNYAAANAFLDG
                     FAQYRRACGLPAITINWGALAEVGVVARNSQVEQLLANAGVQTMYIGHALQHLGRVLQ
                     FDPAQIGVLQVDWKKWKATSGGGGRGSATLFEALFAGDEAGSGGGAMDPRQRLLHELA
                     VLEPPHRIAFMEHLLAEELARILQVPPAQIDYKQNIMHLGIDSLMAVELQTALQSKFA
                     LQVSAMEFIRGLSVAQLASRMLTGMEADLDALAAQILVPDEALDTLLEAETTHLSDAA
                     FEQLLKQAGAGSP"
     gene            18602..19912
                     /locus_tag="VFQ79_21325"
     CDS             18602..19912
                     /locus_tag="VFQ79_21325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HEU0142277.1"
                     /translation="MESRRSEILSRLSATDKRLLLAHLLRQKTPLGNATGEKVAPAFI
                     DDAVLDSDIQPIGPAVCPFTPKAILLTGATGFLGAHLLQELYTRTEAVIYCLVRGRDA
                     ADAQQKLKGNFAQYFAHRLDRERVRLVPGDLAEPRLGLPLETFAILARETDTIFHNGA
                     MLRHLASYAQLKASNVTSTAAMLQLAATDRPKWLHYVSSIVAAVDRDDHGSLTEDFPR
                     RASADLAGGYAQSKWVSEKLLAQAAERGFGVTVFRPGFITGRSDTGVWPNPNDHLVRF
                     ILGCLQMGHMPVSDLTLDMAPVDFVAAAIVRIALSEPTAIPVYNLSNPHPLPWTTLVE
                     WLQSWGYRFEVVSTAAWRERWLSDIDPRNALYPILPLYVGGDTTAQHAELLRKLANVR
                     RSRTVEALSRLQMAFPAPGHDLWRRYMQYFEHAGFLRIESSVGA"
     gene            19933..21009
                     /locus_tag="VFQ79_21330"
     CDS             19933..21009
                     /locus_tag="VFQ79_21330"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU0142278.1"
                     /translation="MLRKLFSRRPQPSPADVPYMDQCTDLPSSLVFIMGCHRSGTSLL
                     YHLLAYTGQVDYVSAYDIIHYDELLHNRITGREAQVKTDLQAVLQRESNRGLDELPVG
                     ADLPEEYRFVLCKEPPKLILNAAKRIDDLFFKPHLTPRTLDAFLDICRKKRFLARADR
                     PLVLKNPADYYFNFRAVHEMLPRAKFIFIHRHPLPMLNSYLHGFRGVLEQPSRYAALL
                     DKRYESLFSGMQLRRKLFLKLFRSGVIGRLLAARLAESFQYYRTNLPKIPQAQYVSIR
                     YEDLCAGPESCLARISRHLQLDLTPRVPPHFIAPRRVPVTDRVRCHYAGRLDEMMPYL
                     EDCGYPAWPEPEGDAVAANCVGDK"
     gene            21013..22023
                     /locus_tag="VFQ79_21335"
     CDS             21013..22023
                     /locus_tag="VFQ79_21335"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU0142279.1"
                     /translation="MVYAATSAADCPDAQYLDRLQALPPSLIFIMGCHRSGTSLLHHL
                     LAYTGQVNYVSTYDVIHYDSLLAHRITGREEAVKAELRERLKTEKNRGLDDLPVGVDL
                     PEEYRFVLTTFDISFSMRSRRRIDEIFAPHLTQETFGKFIELCRKKQFLARRDQPLVL
                     KNPADYYFNFLAVHQMLPDAKFVFIHRHPLEVLNSYLLSFGALMNRQSSYWSMLDGGY
                     RRFFGPAAVERIVAALSMRTGWYARQVLLRLIESFGYYLDNIARVPRDRFVVLRYEDL
                     CLDPKGCLDRIGTHLGLSIEPRIPEGFVAPRHRPVMPRARRQYARQRDALLPYLNHLG
                     YA"
     gene            complement(22086..22175)
                     /locus_tag="VFQ79_21340"
     tRNA            complement(22086..22175)
                     /locus_tag="VFQ79_21340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22139..22141),aa:Ser,seq:gga)
     gene            complement(22207..23070)
                     /locus_tag="VFQ79_21345"
     CDS             complement(22207..23070)
                     /locus_tag="VFQ79_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142280.1"
                     /translation="MSSKRPVTLAFFLFGTGCMMAQPVRVYSEFQRINPFGQVVEIDK
                     AARPREVLSPAVVRNGSSGFHLAITAAPGTTFSLHLGQNPENAADVTVYREIFVQSGK
                     GWIPDGLEKISLPFDGQIGSDGIPGQTTAVFWLDVRLAGDAPVRRIKVEPQVWMSEHW
                     VPYPMEVRVVKARVPKAVAASGPVPRLEERADAPVLAPLRSYVCGTEPRPPTRKGSPS
                     VRELIRRGVEQDLMLARALEKKAGRDTVVKTLLRHAGAATEAEFCGAAAPGSDGEWYL
                     RFRDYLLRESD"
     gene            complement(23075..23293)
                     /locus_tag="VFQ79_21350"
     CDS             complement(23075..23293)
                     /locus_tag="VFQ79_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142281.1"
                     /translation="MKPQEYSERNLEVAGWPVHLTTYKLGDKFYSMADNVSPGACLAR
                     TTADTKEEAEAQALAKAERLLGRTRRIL"
     gene            complement(23290..24165)
                     /locus_tag="VFQ79_21355"
     CDS             complement(23290..24165)
                     /locus_tag="VFQ79_21355"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0142282.1"
                     /translation="MSEYAHPEALVSTEWVAQHLKDPNVRVIEVDVDTSAYNQGHVPG
                     ASAWNWTTQLSDGIRRDVLSKADFERLMNESGIRPETTVVLYGDSNNWFAAWAFWQMK
                     LYGHKDVRFMNGGRKKWISEGRELDTQTPAPAPTSGYQASEADNSLRAFLPQVQGASA
                     KAAAALIDVRSPQEYSGEILAPPGLPETCQRGGHIPAARSVPWGLACNDDGTFKSLAE
                     LQALYGAAGVEEGKPAIAYCRIGERSSHTWFVLKYLLGVKDVKNYDGSWTEWGNLVGA
                     PVERGTGEKSAKGVA"
     gene            complement(24230..26668)
                     /locus_tag="VFQ79_21360"
     CDS             complement(24230..26668)
                     /locus_tag="VFQ79_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU0142283.1"
                     /translation="MAKELVISSNRHETRVAVLEDDQLVEIFFQRSHEYSLAGSIHKG
                     RVTRVLPGMQSAFVDLGLERDTFLYVSDFFEESDEYDRIIPEEKPVKAHSPSLAVASV
                     KVPPPAPPVIAEPAAATPERTDEPEGRDRRSRRSRRRRNRGRGFPESKYATETAPAEE
                     DQEIQETPAVAELDFAVLPGESLAKYRGPEPSEPAGTEEETPDQAPSSHVEALLGDFD
                     GEVTAIEDQPEETAAADDVAEEAMPSEEEEQAEPLLESEAEETEEEDAATVVEDESAD
                     AAEVLDEQEEFDDSEIREMILEAQMDAEARSGPEPEEDELESEDEGSHAAADVEAEEE
                     DDQQPVEEVAAAADEIEPIRKPHSLTATLREQGPRYMHRVARRNRRKTRPSSEEATPE
                     SKQPDYRSDASRTVVRPEKTGPSIADLLKEGQEILVQVAKEPLGQKGARITSHIALPG
                     RYVVYMPTLEHMGVSRKISTDEERLRLKKILQGQRSSMSGGFIVRTAGEGKTEREIAD
                     DMRFLYNLWQDIRQKAEKKRAPALLHHDLDIVQRVLRDQFADSFKAIWIDSEEIYENV
                     LSFVQRFQPVLIPRVKLYTRPAPIFDAFGITQELEKALRPKVWLKSGGYIVINQTEAL
                     VAIDVNTGKFVGKSNRLEDTIVKTNLEAVKEIVRQIRLRDLGGIIVVDFIDMDERKNR
                     QKVMQGLEEAMQSDRAPSKVLQFNDFGLVAITRKRVKQSLERTMCSPCPYCEGAGYVK
                     SVDTVIGEILQEAQKIAGAVDGKNVVLRVNPDVAKVLKSNSNQYLEEMEGILKRPVLV
                     KGDPLLHQEKFDVG"
     gene            complement(27074..>27856)
                     /locus_tag="VFQ79_21365"
     CDS             complement(27074..>27856)
                     /locus_tag="VFQ79_21365"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU0142284.1"
                     /translation="GFHLQVSEFVKLVIILLIARYITDVRKDRLDVIDLLKLGGLVGV
                     PMLLVMRQPDLGTSLTYVPALVVGVFLAGLRWQYMVAIALIFLMVLPIGWHFLRDYQK
                     ARLATFMDPDEDPRGSGYQVIQSRIAVGNGGIWGLGVTQGTQTQLRFRPVPHTDFIFS
                     AFAEEHGFVGVLVGLGLYLMLILQIVQNAQSAVDRSGMYICMGAAAMLLFHVLVNVGM
                     VVGRMPVAGIPLPLMSAGGSNTLSTFMLLGLVHSVRLRRLTN"
BASE COUNT         5370 a         8457 c         8231 g         5798 t
ORIGIN      
        1 ctcaaaatct ggcgggcgcc cgtttgaaag acttgcggac cgttcttgcc tacctgcgaa
       61 cgcggcctga tgtagatggc aatgcgatct cgatttgggg cgattccttt gcgccgtcga
      121 actccgctga gttatatctc gacgagatcg agcaggaagt ttcgccggag atccagtatc
      181 gctcagagcc gttgggagcg ctgatggcgc tgctggccgc gctctatgaa gacggcgtgc
      241 gggctgtcgc cgcgagaggc ggaatcgcga gttacctgac gatgctcgag agcgcgttca
      301 cttatacgcc tatggacgcg gtgctgctcg gagttctgaa agtaggcgat atcgccgaca
      361 ttgtggcggc gactgcaccg aaaccggtac tgttcgaagc cgctgtggac gggcgaaacg
      421 tgattgtcga cgctggaacc ctgggccgta tgtttcccgc ggagcggcgc attaccgtcc
      481 gtcccgaacg cggcgatgtg gcggcgtggc tgattgctca aacaaagccg taagccggct
      541 cagctttcat atacccaacg tccgccgatc attgtgcgct cggtgcgaat gccgatcagc
      601 ttgaatggat ctgtgcgcag gggatcggcg gcgagtacca ccaggtcggc gagtttgccg
      661 ggttcgagtg aacctttcag gcgctcttcg aacgaagcgt acgctccgtg aacggtaccc
      721 acgcgcagcg cttcttcaac ggtgaccttc tgccgcggcc cccacgtctt gccatgagaa
      781 tcggtccgcg tgacgctcga ttgcagcgcc atcatcggct cgaacggtcc gggcgggtag
      841 tcggaggttt gtgtgacggg cactccggca tccagaaaac tgcgcacggc gaacatatgg
      901 ttcagccgct cctccccgta ctcccgcatc ttttcgccgt ggtaataaac gtacgttgag
      961 aaagggttgg gaatggcgcc gagggctttg atgcgccgca ccaggtcgtc cgtgatgacg
     1021 gtgcaatgtt ccaggcggaa acgggcgtcc ttgcgcggat gttctcgctg aaggcgttca
     1081 tacacgcgaa gcgttttgtc gatggccaca tcgccgttgg cgtgcgtgcc gatctgccag
     1141 cctgccaggt gggctctgcg cgcctgctca tacaactcgg gctcgtccgt cacgagaatt
     1201 ccatggtcgt ccgggcgccc gacgtagggc tccgaaagtc gagctgtacg ttctgagatg
     1261 gaaccgtcgc acatgagttt taccgctcca attcgcaccc actcatctcc cattccagtg
     1321 cgaacgccgg cagagatcat ctgatggagg tcggaattgc ggatcaggca gtacacacgg
     1381 atcgcaagct gatccgcttc ccgcgcatcc tggtacgcca gaagggactc ggggccgccg
     1441 agcgcatcat gcgcggaagt cacgccggaa cgggccatca tgcgcgtgat gagttccaca
     1501 ccctggcggt actcctcgcg tgtatgccgg ctcgcgaaaa gcttttgaaa cggcactgtc
     1561 gcgcgctcgc gcagcccgcc cgttaagcgc cgggttgcgg ggtcgcggtc gaaatgccct
     1621 cctgccgggt ccggtgtatc ttctgaaaca ccggcgagtt tcatcgccag ggagttcaca
     1681 taaccggtgt ggccgccgcg atgtccgatg tatacgggat gtccgggcgc ggccgcgtca
     1741 agatcggcac ggttgagttt gcggccgtcg gaggttttcg tgtcgtcgta tttgaacccg
     1801 accacccatt gccccggctc tgttttcgcg gcacgctcgc ggatggcctt ttggatatcg
     1861 gcgatggacc gcagatcgca atccacctgc ttcaggtgac ggataccgga gtaacagaca
     1921 tgcgtgtgag cgtcgatgaa accggggacg accgtgctgc cgcccagatc gatcttccgg
     1981 gttcgtgccg tcgcgaggcg catcacgtct tcatccgagc ccacggcaaa gatacggtcg
     2041 ccagccactg cgagcgcctg tgctcgtgga gcggccggat ccatcgtaag aacgttgccc
     2101 ccgtgtagaa tcaggccggg ctcgatgcgt cccccgaagg cgagcccgcc tccggctatc
     2161 gacgatagcc acgcccggcg cgtgatcgtt cccattgaac ctcctctggt ctcgacagct
     2221 tatcgcgaat ggagtgcgac gcgcgagctt catcgaacgt gcacttatta acatgtctgg
     2281 tgttgccaga ggcgtgtagc gatgtaaaat ggccggaacg cataactggt tggctctttc
     2341 agtctggcga ggggacgaca atgacgactc ttaggatgat tggttgcagc atcgctttgt
     2401 tgagccttct tccggcgcaa gagtatcgcg ccaccttaac gggccgtgtt ctggattctc
     2461 agggtgcgat catccccaac gtgagagtac tggctactca gatcgcgacc aacgcaaatt
     2521 ttgagacatt ttcaggttcc gacggccagt atacgttgcc ttttctgccg cccggcagtt
     2581 accggatcct ggtggaagcg ctggggttca agctgttcgt ccgcgatggc attcgggcga
     2641 gcaccgggga acgcatcggg ctcgacatca ctctggagat cggatcggtc aacgaaacca
     2701 taaccgtgag tgccgaccgg tcgctgctcg aaacggcaac cgcatcgacc ggccaggtaa
     2761 tccgcgaccg gcagatcgag aacatgccta tgaacgggcg cacgccgcta gtgctggcac
     2821 agttggcttt cggagtggtg ccttcgagcg atccgaaatt tacgcggccg ttcgataacg
     2881 caggcccttc gactttctct atgggcggcg ctcccacccg cagcaatgag ctgttgctgg
     2941 atggcgcgcc caacagcacg ggtaatagca gggtggccta taatcctccc gtagacgcag
     3001 tcgaggaggt caaagtcgag accttccagg ctgacgcagc ctacggccac acgggcgggg
     3061 gcaccgtgaa tgtggttatg aaaggcggca cgaatacgct gcacggtaac gtttacgaat
     3121 tcaaccaagt atcgaggttg gctgctacgc cgttcttcac gaaccgcgcc ggattgaaaa
     3181 aacccgtcac acgctacaac cagtggggtg tgaacgttgg cggcccggtt gtgatcccga
     3241 agctggtgaa cggacgtgac aaagtttttt tcttcttcgc gtacgaaggt attaaagacg
     3301 cgttcccaga gccactgacc tcgacggtac ccactgccga gcagcggaac ggcgatttct
     3361 cccaactact tacagtcggc tcgaattacc agatatacga cccgcttacc ggcgtcgccg
     3421 aaggcgcgag ggttcggcgc atgccgttcg caaacaatgt aattccggca catcgcctga
     3481 gcgcgattgc gaaaaactac cttcagttct acccgatgcc gaaccagccc ggacgtgccg
     3541 acggccagga caatttcctt gcgaacacgg tgaggcggga cgatttcaac agcgaattgg
     3601 gccggctcga tttcaacatc aacgatcgcc acaagctctt cggcaatttc cgccacaacg
     3661 aagcgttgct cagccgcggg aaccggttca acaatatcgc gaccggtatc cctttgcgca
     3721 gggtgaactg ggggttcatg gtggacgatg tttacacgtt cacgccgacc atgatgctaa
     3781 acacccgcgc cgcgtggacg cgattcagcg aagcgaacat accccagggc atcggttttg
     3841 atatgaccac tctcgggttt ccttcctcgc tggcagccag ttccaccagg ctcgtactgc
     3901 ctcgggtcgc tttcgatcgc tacacaggtc tgggcgagac ctcgggcaac gatacgccct
     3961 tcgatatctt ccagatcttc tctgcgctca ccaaggtagt tggacaacac agcctgaagg
     4021 ccggcgtgga cctgcgtttg tatcgtgaaa gctcgatcaa tttcggaaac tccagcgggt
     4081 tatttacgtt cgggccggac tggacccgcg gccctctcga taacgctacg gctgcgccgc
     4141 tgggtcagga tttcgcttct tttctgttgg ggcttccgac cagtggaggc tacgacgtga
     4201 atgccgcgcg aacgaatcaa gcgggctatt acgccctttt cctgcaaaac gatttccgcg
     4261 cccgttcgaa tctgacgctg aatctcggaa tccgctacga acgcgacctc cctacgacgg
     4321 agcgatacca tcgatcggtg aacggcttcg atttcgtcac tccgaacccc atcagtgaaa
     4381 gtgcggcagc ggcctacaga ttgaatccga ttcctgaact gccggttgaa caatttcgaa
     4441 cacctggcgg gctgctgttt gccgggccgg accggccgga aatctaccgg acgaaatctc
     4501 actatttcag tccgcgcttt ggtttcgcat ggacgccggc ggcgctcggc ggcaagacgg
     4561 tgattcgcgg cggtttcgga gtgttcttct tcggtctggg caccacgggc atcaacgccc
     4621 tcggattcag ccaaaccaca cctctggttg caaccctcga cggattcctt tcgccggcgg
     4681 cgactctgga caatccgttt cctgacggaa tccgtcagcc caccggctcc gcggcgggac
     4741 tctctacgtt cctcggacag tcggtccggt tctacaattc ttttccgcag aacccttact
     4801 cgaaccggtg gaacatcagc ttccagagag cgcttggaaa caacctggtt atggaagccg
     4861 gctatgtggg caaccacacg gtccatctcg cggtaaacag gcagttgaac tttgtcccgc
     4921 gcgagttcct gagcacatcg ggcgtgcgcg acgccgcggt catcaatcgc atgaccgcca
     4981 acgtcgtcaa tccgttcgcc ggactcattc caggcacccc gctcgacggc ggcaccatcc
     5041 ggcgaagcca gttactgggg gctttcccgc actttataac tggtgacctg atcgccaatc
     5101 gtaccgatgg tatgccggcg aatggcgtga tcctagatgc cagcaacgac ggcagctctt
     5161 actttcacat gctgcaagtc cggctggaga agcggttctc cggaggtctt cagtttctag
     5221 cgaactatca gtattcgaag ctgatcgaga agatcagccg attgaacgat tccgatctga
     5281 gtttagagaa aagaatcgcc gcggaagatc gcccgcagcg gtttgtgatg agtgccagct
     5341 atgagttgcc ctttggaaaa ggtaagccgt ttctggcatc cgagggacca gtccttggca
     5401 gattggccgg cggttggata ctcaatgcga tcttcataac acaacccggg cctcctctga
     5461 attggggaaa cgtgatctac tggggcggtg acctgagctt gaatccgcgc cgcatagagg
     5521 gggcttttga caccacacga ttcaacacga actcgcagca gcaactcgac tggaatgttc
     5581 gcacgttccc gacgcggttc tcaaacttga ggcaggatgg tgtgaaaaac atggatcttt
     5641 cgataatcaa aaatacagcc atcactgaga aaataagtct gcaatatcgc tgcgagtttt
     5701 tcaatgcttt caatcgtccg gcgttcgatc cgccgaatct gtcgccgacg aattccaact
     5761 tcggccgcat tacgaatcag ccaaacctgc ccaggatcgt tcagatggcg ctgcgcttaa
     5821 gctggtaact tatgaagcgt cttgcctttg tatccgcgtt ctccctgctg cttctggttg
     5881 cattacgaag ccaggagcag aaggttttcc agcttgcccc gggtgtgtac ttttggcagg
     5941 gcgacacttc gatccgacgt ccggcgaatt gtacgtgggt tttattcaag gactatgtgg
     6001 tcgtgatcga tgcgaatttt ccctggggcg cacgggagat cctgcccgag atcaagagga
     6061 ccacggacct tccgatccgc ttcgtgttca acacgcatta ccacgccgac catacgttcg
     6121 gcagtggtat cttcatgcag gcgggagcca ccattgtctg ctcgcgggag tgcggcgccg
     6181 aatcccgcac caaaggtgag gcagactggc gcaaccaggt tctgaatcgc agcgagcggc
     6241 ccaatgcctc ggtgcacgat aaggcagtag cggcggaaac tcgcgagaaa ggctaccgcc
     6301 tcgaacaccc aacggtgctt ttcgacggga gtatggcctt tgacgacggt gagcgccgcc
     6361 tggaattgat tcgcatggga ccggcccact cgaagggcga cgcggtggcg taccttccga
     6421 aggaaaagat cctgattacc ggcgatctct gcgtcaactg gaccactgga aacaatgtgg
     6481 gtgaccgtga cgccgatccc gacaattgga ttcgggcgct cgataagctg gtgcagtggg
     6541 atgtggacat cgtggtgccg ggtcacggcc gcccgggcac cacagaaacg ctccggggac
     6601 aacgtgccta tcttgcagag atggttgata aggttcgtgc cggcattcag gcttccaaga
     6661 ccgcggacca attagccagc gagatcgacc tttcccacca tcattcattc ggcgcaaggt
     6721 ccagcgcgaa ttcggtgcgt gccatctatc gcaagctcac ggagccgccc cgttcacagg
     6781 aataatatta tgcgctttcc agccatcctc gtctgtttat tcgcgcctgc cttcgcgctc
     6841 gccgcggact ctctgcccgc ccgctacttc cgcctcatgg aggccgggac ggcccgcgtg
     6901 gaagcaaagc tgaaagccgc tcccgatgcg gacctgaaga cgcttgaagc cgagtcttcg
     6961 tggcggcact tcccctacgc gattctcgca ccggccgtgc tctactcgaa gcagcatcgg
     7021 gataacccgc gctatcgtga caagcacatg cgggatctcg ccattcgcat cggcgacatg
     7081 ctggcaggtg agagcgagaa gggtacattc gaaccgcggc tcgatagcga ttgggacacg
     7141 tacatgtggt tggatgcgta tcgtctgctg gaacgcgagc taggcgacga gcgccgcgag
     7201 cgctggaagc gtgaaatccg aaaaaacatc gctatgcagg tcgagggcac gcgcgagcgg
     7261 cttgatttcg cgtggtacaa ctcgccctac atcggcacat cccccaatca ctacgcgtta
     7321 tgggcggcga acctttacct gggtggccgc ctcttcggag acaaagaatg ggaagatctc
     7381 ggccgcaccg tgctgcgccg tttcgctatg gtggagcaga cgcccgacgg ctactggggt
     7441 gagcacagcc gttcgggtcc gaccatcggc tacaaccacc tgacattgag cgccgtcgcc
     7501 atgtactggg aattcagcaa agataaggac gtgctgcccg cgctgcgccg cgcaacggat
     7561 tttcacaaga actgcacgtt tctcgacggc acgccgatcg acgtattcaa cgaccgcaat
     7621 cgctattggg gcgtgagtgc gtgggcccag tttgcattct cgaattttcc ggacgggcgt
     7681 ggttacgccg cttttctgac cagctttttc gatcctgaga agctcacggt cgatcagttg
     7741 ggacggctcg cgcaggacgc tctctactac catgaaggtc cgtcggaggc tgcgccgcaa
     7801 cagcgcccgt cgtattccta ccggatgagc gtaccggccg gtgtgcgaaa aaccgggccc
     7861 tggcaggtag cgctgtccgg cctcatcgat acgcagacca tcaacaatcg cttctatctc
     7921 gaccgccagg gacacctcag tatttttcac cagaaggcgg gcctgatcat taccggcgca
     7981 aactcgaagc ggcagcctga gttggcgaca ttttccgaag aactgatggg gcaggtcgtg
     8041 catatgcccc tcagcagccg gcttcagatg tcggatacgc aagaccgtct ctctctggcc
     8101 tataacacgt ttttcagcga cctctacgtc atgcctcctt cggacagcgg gcttgatttc
     8161 cggttcgtga tcaatggccg tggtacacct gcgaaggatc cgaaattgac gctccaactc
     8221 gtcctcgaac ctggagagga actggaaaca ggctccggcc ggaaggttac ggtcggccag
     8281 gacaaaatcg aattcggcgg cagcgagttg ggcggctcga tacggcatca tggctggact
     8341 ctgtctgtcg atcgaaccgc tcaactccgc tggcctgtct acccgcataa cccatactcc
     8401 gatataccgg agaccaatct ccgaagggcc gttgcggtgg tggtcgtgcc gctcaacctg
     8461 aagccccggc cgggcaaata cgtccgtccg cgcgatcagg aaatcgagtt caaactctcc
     8521 gtaccctaat gattatgcga actctcttca tcgcggcctt ttggggagcc gccggcttgt
     8581 acgcccaatc cctcgacata ccaatcaccg ttgaggaaac agcaggtatg gcgcgcaaag
     8641 ccgaacccgt tacgttcggt gtgccgctgc cacgcggagc actgcgggag gctgcccagt
     8701 tgcggcttta caatccggaa ggcgaggtga ttcccgccgc cttccgcgta gtgaaccgct
     8761 ggtgggatga cgctcaaaca caggttgctt ccatccaatg ggttcacgtg gacttcttcg
     8821 ccgacgttcc ggcgcaaggc cgtgccgtgt accggttgcg caactcccgc gcgccggagg
     8881 cgccgcctcc cgcccgtctt cgcgtggaga cggtggggga cgatatcaaa gtggacaccg
     8941 gtccgcttca gttcaccgtc catcggaacg gaccctttct ggatgcgcct gggtttggcg
     9001 gcgcggactt ccttctgcgt tccgacgagc gcatttacaa ggcgggcaac tggaaggatt
     9061 cgcagttgga agtcgaggaa gaaaacccgc ttcgcattgt attgaagcgc accggctcgc
     9121 acggctgggt ggataaagaa gaccgtgcgc tggactatgt gctccgtatt gtggcctacg
     9181 ccggccaacc gcagatccgg ctcacttata gcttcatcaa caggcaggga cgcgcgatgg
     9241 ccgattttgt gaggctggac ggcctctggc tgcaggctcg tcttgacacg gatacgccgc
     9301 cgatgcgcat cgaacaactc caggcggaac cgcggcgccc cggctggttt gaagccggtc
     9361 caatcggtat cggcttgcgc tggttctggc aattgtaccc taagggcttc gaaatccgct
     9421 ccgatcgcac gttgcgtctg gctcttttcc ccgagacggc gcggccgcag aacatctata
     9481 ccggcgtggc aaagacccac gaaatggccc tctcgttcgg aggcggaaat ctttccgcgc
     9541 aactggacca tccgctctac gcggttgcac cgcccaaatg gtatacgcgg gacacgcgcg
     9601 cattgggccg cctggtggaa tcgtcaccgg aggcgatccg gccggaatac tggccgctgg
     9661 tgcagaagta tgatcgatgg ctctccacgt gccgggacgc ggtgctcgcc aagcgcgacc
     9721 gcggctatga gtttgaggga cggcgtctgg acgaatacgg aatgctcaac ttcggcgatg
     9781 cgctgcacaa gctcatccgc aacgaccgga ggccggatta cggaattcat tgggataccg
     9841 aatattacga ttttccgcac gcgctgtttc tgcacttctt ccgcacggga gatctcaagt
     9901 cgctgcgtac cgcagtggag gcggccgcgc atcttgccga cgtcgacatc tcgcactacg
     9961 aggcgggcaa agattgcccc ggcgcggcgc gcaccggtcc cggtctgaat cattggaccc
    10021 gctattcaaa cggggttttc atctcgtcga ccagttgggc cttctataag aatgaaggat
    10081 tgttcgaccg ctatctgctc accggcgatc tctggtcccg cgatgtagcg aggatgtcgg
    10141 cggactgggg catctgctac aacgggctcg accttcagag caatacacga tccatcggcc
    10201 atggtctttt tgcgatgctc aaagcgtacg aagttttcgg ggacaagaag tatctcgacc
    10261 gcgcgcaatg gatcgtcgac aatgtccacg cgtggcagga cggagacgtg gaaaagttgc
    10321 gtgtcctgaa cccgcgcgcg gtgtgggacc cgcagttcaa gggcggatac agccaccagt
    10381 cgtggatgta tggcattgcg ctggaagcga tggcgcaggc atcccaaacg ctggcacgtc
    10441 ccgaaatgcc cggctacatg cgccgtgccg ccgattggat tttcgggaat ccgcgcgagt
    10501 gggaccccga acggcggata ttccggaacg cccctgttca ttccgtgatg cttacacctg
    10561 gacttaccta tatcgccgag acgagcggag aaaagaagta ttgggacgtg gcgctcgaga
    10621 gctttaccag gcagaccgaa gccggcgaac tgaccgaccg gctcaaactg ttcgcccaac
    10681 tgttccgcaa ctcgcagcgc ttcccatggt atttgtcagt ggaaggaccg ccgcgttttg
    10741 ggggcggtac ggaacaaacc agacgccgcg cgcacggagg tttctagcct taatctggat
    10801 ccggcacatg cactgcaaga acaggtaaag aaaaagagcc cgccttgact tcattcttcc
    10861 ggggggaata cactttcatc cactggagat gctatgagtc atgaaacggc gatagaggct
    10921 agaactgtct ctgcgcaaaa gccgaaacgc tacgtgaaga ctgggccact tctagccggc
    10981 tacctgctct cccgaatgag cctcttgcac ccgctgatgc cggccagggt gcgctacaag
    11041 ctccctactt cctggaattg gaagatgcta tcgacgttcc tcggcagtgg ccgcagccag
    11101 gtctacatcg atgtgcacac ccgactggat ccgccggcgt cctatcagcc gctggtcacg
    11161 accgattcgc gctggcaact ctcggaggcg gacatccgcg ggttttggga gcgcggcttc
    11221 atcggaccct tgaagctcat ggaaccggaa gcgatgcggg atattgcgcc gcgtatgtgg
    11281 cggctatggg aagctgaatc gcgtatctac ccgccgaagt cctacaagta cgtgggatcg
    11341 acagccaccg gcagcgacaa gacggagatc agcaacgagg cttacgctac caagggtctc
    11401 aacgcccgcg ataagcattt gcaggacgat gaactgatgt cgctctatgc gcaccccgcg
    11461 attgtggagc gcatggccca actgctcggt cccgagttgc tcctttggag aagccaattc
    11521 ttccccaaat atcccggcat gggcggcaca ggatggcacc aggcaacctc gtatcttaac
    11581 gaaacgttcc gcactgccac gttgacaccg cgcaacctgc gaaagctgtt tcaactgacc
    11641 gcatgggtag ccgtcacgga ctcaaccgtg aggaatggct gcatgcggtt cattcccggc
    11701 acgcattggg aactcctgcc gatgaccgtc gaagaatacg atcccatcaa acacgccggc
    11761 aataagcacg accgcttcgg cacgaaaatc atgcgtcccg cgctcggcga cctggaaaag
    11821 cgcgccgtca atctggagat gaaggctgga gagttcgtca tcttctccga acgcaccatg
    11881 cacggcgcgc tgcccaacgt caccacggac gatgcacgcc tgggcatgtc ggcgcgctac
    11941 attgtccccg acgtcaagat ccacaatccg tgggtactcg gcgacggtgg tctttctatc
    12001 gtttacctgc agatccagaa gcttaatctg gaccgctgga agatcatcca actccgcggc
    12061 gacaagaaag gcccgatggc cgatcgagtg atcccagcgc ccgaaggcgc ccgccgctgg
    12121 tagcgcatca gaaaggagtg ttgccgatgt ccgtgggcga gagtctcgcc tccggcatgt
    12181 ccgaaccgat tgccatcgtc ggtatgggct gccgttttcc ggcgggcgcc tcgaatccgg
    12241 aggcgttttg gagattgctc tgcaacggaa tcgacggaat aaccgaggtg ccggccgagc
    12301 gctgggatgc gcgtcgtttc tatgaccccg atccggcgaa gcccggcaag acgtatgtca
    12361 agcagggtgg atttctgcgc gaaagagtgg agtacttcga tccactggcg tttggcatct
    12421 cgccacgcga ggcgcctgcc ctggacccgc agcagcgcct gctgttagag gtgacctggg
    12481 aagccctcga agatgcaggc ttgaggatag agtcattggc aggttcagcc acgggggtgt
    12541 ttatcggggc gttcacgctg gacatgaaga tgttgcagtc gaacattcac aaccgtgacc
    12601 ggctgacgtc ccatagcacc accggatcca gcatgaccct gatggcgaac cggctctctt
    12661 atgtgttcga ctggcgcggt ccgagcatcg cgatcgatac ggcctgttct tcgtctttgg
    12721 tggctgctca ctacgcgtgc cagagcctct ggcgcggcga atgtactctc gcgatcgccg
    12781 gcggcgcgaa cacgatgctg cgtcccgagt atttcattgt gatgagcaag ggacactacc
    12841 tgtcgccgcg aggccgctgc cacacgttcg atgcgcgcgg cgacggttac gcacgaggcg
    12901 aaggcgcggg tgtcgtaatc ttcaagccgc tttccgccgc cctgcgcgac cgtgacgcta
    12961 tctacgccct cgtccgcgcg accggcgtca accaagacgg gcaaacggcc ggaatttcgc
    13021 tgcccaacgg tgaggctcag gaacatctca tcgcgtcggt ttaccagcag tcctgtgtaa
    13081 caccgggcga ggtgcagtac atcgaggcac acggcaccgg tacgaaagcg ggagaccccg
    13141 tggaagcgcg cgctctcgat cgggccttgt cggtgggccg cacaccgggg catcgctgct
    13201 tcgtaggatc agtgaagacg aacatcggtc atctcgaggc cgcggccgga gtggcggggc
    13261 tgatcaaggc cgcgctgtgt ctcgaccgtc gccagatccc gcccaacctg catttcgagc
    13321 agccaaaccc ggacataccc ttcgacgaaa tgtgcattca ggtccccacc aggatcgagt
    13381 cgtggccggc atatgggaca cgcgcgtatg ccggcgtgaa ttcattcggt tatggcggca
    13441 cgaatgcaca cctgttgtta gaagaagccc ccgtccgttc aacacaagcc ccatctgcgg
    13501 aattgagccg cccactgctg tttcctatct ccgctcgcag cgagcaggcg ctgaaatccc
    13561 tggctgagag ctacgccgca ttcttgaatg agggggcatc cctggcggat ttctgccaca
    13621 gcacgagtct gcgccggagc caccattacc ggcgcgcggc gctggtggcc gagacgcgcg
    13681 acgagttgtg cgaaaagctg ggcgccttgg cggccggagc gctactgccc ggtatgaatg
    13741 tcaaccagac gctacccggc gacgcgtgcc gcctggtctt cgtttatacc ggcatggggc
    13801 cgcaatggtg ggccatggga cgcgagttga tgggcacgga ggcggtcttc cgggagtgcg
    13861 tcgaagaatg cgatgacctc ttttccagct atgccggatg gtcgctgctg gacgctctca
    13921 acgctccgga agagtcgtcc cgcatgcggg aaacacaggt tgcgcaaccg gccaacttcg
    13981 tcctgcaggc cgcattgacg gccctgtggg aatcgtgggg gatcaagccg gatgcggtgg
    14041 tgggtcacag caccggagaa gccgcggctg catatgccgc cggtgcgctg tcgctccaag
    14101 acgcgcttct ggtgagttat caccgaagcc gcctgcaaca gatgcaggcc ggcctgggca
    14161 gcatgttggc ggtgggcctg tctgaaggga acatcgccga tgtcatcagg ccgtatgacg
    14221 gccgtgtcga tattgccgca ataaacggcc gttcagccct tacgctggcc ggcgatacag
    14281 ccgcactgca tcagattgcc gccgcgctcg aggagcgcga agtgtttcac cgcttcctcc
    14341 gcgtcgacgt ggcctaccac agcgagcaaa tggatgcggt gcgcgagccc ttgttgaagg
    14401 cccttgcggc gattcaacct cgcgacactg cgataccgtt ttactcgagc gtcaccggcg
    14461 cccggacgcc aggctccgaa ttgacggccg cgtattggtg gcagaacgtc aggaagccag
    14521 ttcgtttcgc gcaggcgatg aacactctgg taggggatcg gtaccacgtg tttgtcgagg
    14581 ccgggccgca tcccgtgctg caaaacgcca ttcaggaagc cgtgcgtgcg gccggcggcg
    14641 cgggggaggt catcgcttct ctgcaccgcg agcgtccgga gcgtgcgacc atgctgagcg
    14701 ccctgggtgc gctttatacg ctcggttttt ctcccgactg gtctgcgttg accccacccg
    14761 aagggcaata catcaagctg cccacttacc cgtggcagcg cgaatattac tggcacgaat
    14821 ccggtatttc gaagctggac cgtgtcggcc agccgggatc tgtgtttttg gatacgcctc
    14881 tggctctccc catccccgcg tgggaggtag agttgaatag taactacttc ccgtatctgg
    14941 ctgaccactg cctcgacaag acgaccatct tccccggggc ggggtatgtc gaggcgggtc
    15001 tcgcgctgca ccaggcatta cgtccgcaag acgtgtacac gctcgaaggc gtggagttcc
    15061 agaaactgct tgtggtggat ccgaaagata cggctatcct gcacgtgcga tgcgatccca
    15121 atacgcacga gttcaagatc ttcagccgcg cgaagcatga cagcgccgcc tgggaattgc
    15181 acgccctggg ccgattaatg cccggctcag tccggcctcg gccccgcgta tcgccggccg
    15241 aactcagcgc caagtgtccc gaggaaatca gcgcggcggg cttttatgag cggctggagt
    15301 tgtcgagatt ctattacggc ccttatttcc aaacgctcaa acgcatctgg atcgggccca
    15361 gttcaggatt tgccgggatc caggggcatg cagccctggc gcaggatccg gcgccgtacc
    15421 ttgtgcatcc gacgctcctt gacggcgccc tacaacttct ctcgttaaac gcgccgctgc
    15481 gggcctcgtg tccgtgggtg ccgaggtcca tcgaccggat cgtggtttat cattcgcctg
    15541 cctctatctg ctgggcctac gcggaagcgg tggaacgggg gcaaggcacc ctgcgcggga
    15601 acatcgtcct ctgcgatgag gacggtaatg tgaccgttga aatgacgggt gttcattgcc
    15661 acgaaatcac caccttgccc gcgcaggaag catggcagca gtccctgtat agctggagct
    15721 ggcagccggt cgccggggag ccggcactcg ccacacctgt tcacaccggg ggctgcctcg
    15781 ttgtgaccga tggcagcggc gggctggaga cgattattac ggcagctttg cacgcgcacc
    15841 aggtgcccta ccgggtgacg gaacgtgccg agccgcggga attccgcacg atcctgtatc
    15901 tgtgtactgc ggcggagcca caggatgcat cgtccggcga cttcgatacg gctgcgcgtg
    15961 agtgcaccag gctgctggaa ctgttgcatt cgcttccggc ggagagcctc gatgccggtg
    16021 tggcgctgag catcgtcact cgtggcgcac agtcggtgac gccggaggat cgcgtgactg
    16081 cccgggccgc gaattctgtc tggggcctcg gacgtgtgat ccggaacgag tatccaacgg
    16141 ttgcgtgcca gttgatcgac cttgggtccc gccccaccgc gcacgactat gcgcggttag
    16201 tcgagcacgt cttgcggcag actccggaac ctgagatcgc ggtgcgcggt ggcaccacgt
    16261 tcgtacaccg gctgcagcac gcaccatata acgtcgaaac cgccgaagcc caagccgtta
    16321 atactgacga gccggtggaa cttgtgattg gagcgccggg gcggctcgac agtctgcatt
    16381 accgcttgtc acaacgccgt gttcccgggc cgggggaggt ggagatccgc gtacaagcgt
    16441 cggctcttaa ttacaaagac atcctcaaag ccatggggac gatctccaac aaggtactgg
    16501 aaggcaccta cttcggatcg gctttcggga tggaactatc cgggacggtt gtagcaacgg
    16561 gtgagggagt cggggacgtt cgggtaggcg acaaggtgat cgccacgacg aatcaaggca
    16621 gtttcaggtc ttatgtcact acctcggcaa cctatgtgat tccgaaaccg gtttctctga
    16681 gcatgcagga agcgcctgca ttcacggttt tcctgactgc ctactatggg ctgatggatg
    16741 ttgcgcgcct cagacccggt gagcgcgtcc tcatccacaa cgccgccggg ggggttggcc
    16801 tggctgccgt gcagattgcg cagtggctgg gcgccactat atatgccacg gccggaagcg
    16861 aggagaagcg agccagtcta cgggcaatgg gagtgccgta tgtgatggac tcccgaagca
    16921 tactgttcgc cgatgagata tgggcattga ctgccggtga aggagtcgat gttgtcctca
    16981 acgccatggc gggcgaggca ttgcgcaaga gctttgccct gctggcgccg tacggccggt
    17041 ttgtagaaat cggcaagaag gacattgcgg agaacaacgg tttacccatg cagacgttcc
    17101 accgcaacgt ctcgttcacg gccgtcgatc tggaccagat ctttcgcgat cgagtcacat
    17161 tggcgcagcg cctgtttcgc gacgtggccg acgggttcga tcgcggccat tttcgtgccc
    17221 tgcccactac cgtatttccc gccggagagg cggagaacgc tttccggtat atggcgcaga
    17281 gcaagcacac cggaaaagtc atggtcgatt tctcgccgca accggtgaac gcgttcccgg
    17341 tggccgcggc atgctctccc attcgtcatg actccacgta cgtggtaacg ggcggaaccc
    17401 gcgggttcgg actcgagatc gcaaaatggc tggtcgccga aggcgcccgc taccttgttc
    17461 tgatcagccg cagcggtgct gccggagacg acatcaaaca ggcgaaggcc gtcatggagg
    17521 gccagggcgt gcgggtgatg gtggcagccg ccgatgtcgc tgccgcggac caggtccacc
    17581 gcctgtggcg gaacattcag acggccatgc cgccggtgcg gggcgtgatt cacggggcga
    17641 tggtgctcga tgatgtgctg cttaccggtc tcacggcgga acgcttgcgg caagtcatgg
    17701 cgccgaaggt tctcggggct ttgcatctgc acgagctgac caaggaaaca ccgcttgact
    17761 tctttgtgat gctctcctcg gtcgcgtccc tggtgggtaa tgtgggacag ggcaactatg
    17821 cggctgcgaa cgcgttcctg gacggattcg cgcagtaccg gcgcgcgtgc ggactgcccg
    17881 ccatcacgat taactggggc gctttggccg aagtcggcgt agtggcccgg aattcgcaag
    17941 tggaacagtt gttggcgaac gccggcgtgc agacgatgta catcggccac gccctgcagc
    18001 atctcggccg ggtgctgcaa ttcgatccgg cccagatcgg tgttctccag gtggactgga
    18061 agaaatggaa agccacaagc gggggcgggg gcaggggcag tgcaacgctt ttcgaggctc
    18121 tgttcgccgg agacgaagca ggttccggcg gcggcgcgat ggatccgcgg cagcggctgc
    18181 tgcacgagtt ggcagtgctc gagccgccgc accggatcgc cttcatggag cacctgctgg
    18241 ccgaggaatt ggcgcgaatc ctgcaagtgc cgccggcgca gatcgattac aagcagaaca
    18301 tcatgcatct cggcatcgat tcgctgatgg cggtggagct gcagaccgcg ctgcagagca
    18361 agttcgcact gcaggtttct gccatggagt tcattcgcgg gttgagtgtt gcgcagttgg
    18421 catcgcggat gttgacagga atggaggcgg atctcgatgc gctggccgcg cagatcctgg
    18481 tgccggatga ggcgctcgat accttgctcg aagcggaaac gacgcatctg tccgatgcgg
    18541 cattcgagca attgctgaaa caggccggcg cagggtcgcc gtaatccgga gacggggaat
    18601 catggaatct cgaagatcgg agatcctttc gcggctgtcc gcgacggata agcggctgct
    18661 gctggcgcat ctcttacgtc agaagacgcc gctcgggaat gccacgggag agaaggttgc
    18721 accggctttc attgacgatg cggttctgga ttcggacatt caaccgatcg gaccggcggt
    18781 gtgcccgttc acgcccaagg caatattgct aaccggcgcc accggcttcc tcggtgctca
    18841 tctcctgcaa gaactgtaca cccgtaccga agcggtcatt tattgtctcg tgcggggtcg
    18901 ggacgcggca gatgcacagc aaaagctgaa ggggaatttc gcgcagtact ttgcgcatcg
    18961 gctcgaccgg gagcgcgttc gtcttgttcc aggcgatctg gccgagcccc gactcggcct
    19021 gccgctggaa acgttcgcaa tactggcgcg tgaaacggat acgatctttc acaacggcgc
    19081 gatgctccgc catctcgcat cgtatgcgca gctcaaggcc agcaacgtaa cgagcacggc
    19141 ggccatgttg cagcttgccg ccacggaccg gccgaagtgg cttcactacg tttccagcat
    19201 cgtcgcggca gtggaccggg acgaccacgg ctctctgacc gaggatttcc cgcgccgcgc
    19261 ttcggcggat ttagccggag gctacgcgca atcgaagtgg gtatcggaga aactgctggc
    19321 tcaagccgcg gagcgcggat tcggagtgac ggttttccgt cccgggttca tcaccggacg
    19381 cagtgacacg ggcgtctggc ctaacccgaa cgatcacctg gtgcgcttta tcctgggctg
    19441 cctgcagatg gggcacatgc cggtgagcga tctcacgttg gatatggctc cggtcgattt
    19501 cgtcgccgcg gcgatcgtgc ggatcgcgtt gagcgagccc accgcgattc ccgtatacaa
    19561 tctttccaat ccgcatccgc ttccgtggac gacgctggtc gaatggcttc aaagctgggg
    19621 gtaccggttc gaggtagtgt ctaccgcggc ttggcgcgaa cggtggctca gcgatatcga
    19681 ccccaggaac gcgctctacc cgattttgcc gctctacgtg ggcggtgaca cgacagcgca
    19741 gcacgccgaa ttgctacgga agctggccaa tgttcgccgc tcgcggaccg ttgaagcgtt
    19801 atcgcggctg cagatggctt tcccggcacc cggccacgac ctctggcggc gttacatgca
    19861 atacttcgaa cacgcgggat tccttcggat cgagtcttcc gtgggggcat agtcccgagg
    19921 gggaaacagg gaatgcttcg aaagctcttt tcacgccgcc ctcagccttc tccagcggat
    19981 gtgccgtaca tggatcagtg cacggacctg ccgtcctcgc tcgtcttcat catggggtgc
    20041 caccgctcgg gcacgtcgtt actatatcat ctgctggcct ataccggaca ggtcgactac
    20101 gtcagcgcct acgacatcat ccactacgac gaactgctgc acaatcgcat caccgggcgg
    20161 gaagcacagg tgaagacgga tcttcaggcc gtgctgcaga gagagtccaa ccgtgggcta
    20221 gatgaattgc cggtaggggc agatctgcct gaagagtatc gtttcgtttt gtgtaaggaa
    20281 ccgccgaagt tgattttgaa cgccgccaag cgaatcgacg acctcttctt taagccgcac
    20341 ctcacgccgc ggacgctgga tgcctttctg gatatctgcc gcaagaagcg cttcctcgcg
    20401 cgcgcggacc gtccactggt gctgaagaac ccggccgatt attacttcaa tttccgggcc
    20461 gtccatgaaa tgttgccgcg ggccaaattc atcttcatcc atcgccatcc tttgccgatg
    20521 ttgaattcct acctgcacgg cttccgcggc gtcctcgagc agccgtcaag gtacgctgcc
    20581 ctgctcgata agcggtacga gtctctattc agtggcatgc aattgcggcg caagctcttt
    20641 ttgaaactct ttcgttccgg cgtcatcggc aggttactcg ccgcccgcct tgccgagagc
    20701 tttcagtatt accggactaa tcttccaaaa atcccccagg cgcagtacgt gtcgattcgc
    20761 tatgaggatt tgtgtgccgg tccggaaagc tgtctggcgc gcatcagccg ccatttgcaa
    20821 ctcgacctga cgcctcgcgt cccgccccac ttcattgctc cacggcgcgt tccggtgaca
    20881 gaccgcgttc gttgccatta cgccggccgg ctcgatgaaa tgatgccata cctggaggat
    20941 tgcggatatc cggcgtggcc cgaaccggaa ggcgacgccg ttgccgcgaa ttgcgtgggg
    21001 gacaagtgaa ggatggttta cgccgcgaca tccgccgccg attgtccgga cgcgcaatat
    21061 ctggaccggc tgcaagcgct gccgccatcg cttatattca tcatggggtg tcaccgctcg
    21121 gggacttcgc tgttacatca tctgctggcg tataccgggc aggtgaacta tgtctccacc
    21181 tacgacgtga tccattacga ttccctgctg gcccatcgga ttacgggccg tgaggaggcc
    21241 gttaaagccg agcttcggga acgcctgaag actgaaaaaa atcgtgggct cgacgacttg
    21301 ccggtgggag tggatctccc ggaagagtac cgattcgtcc ttaccacttt cgacatctct
    21361 ttttccatgc ggtcgcggcg ccgtatcgat gagatcttcg ctcctcacct gacccaggag
    21421 actttcggaa agttcatcga gctttgccgc aaaaagcagt ttctggcccg gcgcgaccag
    21481 ccgcttgtgc tcaagaatcc ggccgattac tatttcaatt tcctggcggt tcaccagatg
    21541 ctaccggacg cgaagtttgt ctttatccac cgccacccgc tggaagtgct gaactcgtac
    21601 ttattgagct ttggcgcgct gatgaaccgg caatccagct actggtccat gctggatggt
    21661 ggttaccgga ggttctttgg gccggcggcg gtggaacgga ttgtcgccgc actcagtatg
    21721 cgcaccggct ggtacgcccg gcaggtgctg ctgcggctca tcgagagctt cgggtattat
    21781 ctcgacaaca tcgcccgcgt cccgcgggat cggttcgtgg tgttacggta tgaagacttg
    21841 tgcctcgatc cgaaaggctg tctggaccgc atcggaacgc acctcggcct ttcgatcgag
    21901 ccacgaattc cggaaggatt tgtcgcgccg cgccatcggc ctgttatgcc gcgcgccaga
    21961 cggcaatatg cgcgccaacg cgatgctctg ctgccctact tgaaccattt agggtacgcg
    22021 tgaagtgaag ttctcttact tggttatggg ctgcgaaatg gcaagcagcg aatcgtaagt
    22081 tattgtggcg gagagagagg gattcgaacc ctcgatagag tttcccctat acacgctttc
    22141 caagcgtgcg ccttcaaccg ctcggccatc tctcctgtgg cagcgatttc agtttatcac
    22201 agggggttag tcggactccc tcaacaggta atctctgaag cgcaggtacc attctccatc
    22261 gcttcccggt gccgccgcac cgcagaattc agcctccgta gctgctccag catgccgcag
    22321 cagagtctta acgacggtat cgcgccccgc cttcttctcc agtgcgcgcg ccagcatcaa
    22381 gtcctgctcc actcccctgc gaatcagttc tctgacactg gggctgccct ttctggtggg
    22441 cgggcgcggc tccgtgccgc acacatagga tcggagcggt gccagaaccg gcgcgtccgc
    22501 cctctcttcg agacgcggca ctggtccgga tgcagccacc gccttgggga ctcttgcctt
    22561 caccacgcgg acttccatcg ggtacggaac ccagtgctcc gacatccaaa cctgcggctc
    22621 cactttgatc cggcggaccg gggcatcacc cgccagccgg acgtcgagcc agaaaacagc
    22681 ggtcgtctgt cccggaatgc cgtcggagcc gatctgcccg tcgaaaggta gtgagatctt
    22741 ttccagcccg tcgggtatcc agcctttgcc ggattgcaca aagatctcgc gataaacggt
    22801 gacatccgcc gcgttttcag ggttctgtcc aagatgcagc gagaaggtgg ttccgggcgc
    22861 ggcggtgatg gccaggtgaa atccggagct gccattccgc acgacggccg gagaaagcac
    22921 ttcccgtgga cgcgcggcct tgtcgatctc taccacttgt ccaaaaggat tgatccgttg
    22981 gaactccgaa tagacgcgca ccggctgcgc catcatgcaa ccggtgccaa acaggaagaa
    23041 tgcgagtgta accggtcttt tagaactcac cagttcataa tatccggcgc gtgcggccga
    23101 gcagccgctc cgctttcgcc agggcttgcg cctccgcctc ctctttcgta tcagcggtgg
    23161 ttcgcgcgag acatgcgccc ggcgacacgt tgtcggccat cgagtagaat ttgtcgccta
    23221 gtttgtacgt ggtcagatgg acgggccagc ccgccacctc cagattgcgc tcagagtact
    23281 cctgcggctt catgcgactc ctttcgcgct cttctcaccc gtgccccttt cgacgggcgc
    23341 gccgaccagg ttgccccact ccgtccagga gccatcgtaa ttcttcacgt ccttcacgcc
    23401 cagcaggtac ttgagaacga accacgtgtg gctcgaccgc tctccgatgc ggcaatacgc
    23461 aatggcgggc tttccctctt cgacgcccgc cgcaccgtac agtgcctgca attcggcgag
    23521 ggacttaaac gtgccgtcgt cgttacaggc cagtccccag ggcacgctgc gcgcggcagg
    23581 aatgtgcccc ccgcgctggc acgtttcagg tagtcccgga ggcgccagga tttccccgct
    23641 gtattcctgc gggctgcgca cgtcaatcag agccgctgcc gcctttgccg aggcaccttg
    23701 cacctgcggt agaaacgccc gcagcgaatt gtcggcctca cttgcctggt aaccgctggt
    23761 gggcgcggga gcgggtgttt gggtgtcgag ctctctcccc tcggaaatcc acttctttct
    23821 cccgccgttc atgaaccgta catccttgtg gccgtatagc ttcatttgcc agaacgccca
    23881 ggccgcaaac cagttgttac tgtctccgta aagcaccacc gtcgtttcgg gacgaatgcc
    23941 ggattcgttc atcagccgct cgaaatcagc cttggaaaga acgtcccggc ggatgccatc
    24001 ggacaattgc gtggtccagt tccacgcact cgctcccggc acgtggccct ggttgtaagc
    24061 cgaagtgtcc acgtctactt caatcacgcg gacattcgga tctttcaaat gttgtgccac
    24121 ccattcggtc gacaccagtg cttcaggatg cgcgtattcg ctcatcatag cctcctctat
    24181 acacccctaa cattatcgag attgcagaga ttgcgcaagc tcactgcctc taccccacat
    24241 cgaatttctc ctgatgtagc agaggatcgc ctttcaccag aacgggcctt ttcagtattc
    24301 cttccatctc ttcaaggtac tgattactat tggatttcag caccttcgct acatccggat
    24361 ttacccgcag gactacattt tttccgtcca ctgcacctgc tatcttctgt gcttcctgga
    24421 gaatctcgcc tatcaccgta tctacgctct tgacgtatcc ggcgccctcg cagtacgggc
    24481 agggcgaaca catggtcctc tccaggctct gcttcactcg tttgcgggtg atggccacca
    24541 gcccgaagtc attgaactga agaactttgg acggcgcacg gtcggactgc atagcctctt
    24601 ccagcccctg cattaccttc tgccggtttt ttctctcgtc catgtcgatg aaatcgacca
    24661 cgatgatacc gcccaggtcg cgcaaacgaa tctgccggac gatctccttt accgcttcga
    24721 gattggtctt tacgatcgtg tcttcaaggc gattggattt gccgacaaac ttgcccgtat
    24781 tcacatcgat ggccaccagg gcttcggtct ggttgattac gatgtagccg cccgatttca
    24841 accacacttt gggccgaagc gctttctcca attcctgggt aatgccgaac gcatcgaaga
    24901 taggcgccgg ccttgtgtag agcttcaccc tcggaatcag caccggctga aaacgctgca
    24961 caaacgatag aacgttctcg taaatctctt cactgtcgat ccagatcgcc ttaaacgagt
    25021 ctgcaaactg atcgcgcagt acgcgctgca caatatcgag atcgtgatgc agcagggcgg
    25081 gcgcccgctt cttctcggcc ttctgccgga tgtcctgcca caggttgtac aggaaccgca
    25141 tgtcgtccgc gatctcccgc tctgtctttc cctcgccggc agtccggacg atgaagcctc
    25201 cactcatact ggaccgctgt ccctgcagga ttttcttcag gcgaaggcgc tcctcgtccg
    25261 tcgagatctt ccgcgaaacg cccatatgct cgagcgtcgg catatacacg acgtaccggc
    25321 ccggcaatgc gatatgggag gtgatccgcg cgcctttctg gcctagtggc tccttggcca
    25381 cctgcaccag aatctcctgt ccctctttca gaaggtccgc gatcgagggc cccgttttct
    25441 ccggccgtac aacggtccgt gaagcgtcgc ttctatagtc cggttgctta gattcgggcg
    25501 tagcctcttc actcgaagga cgcgtttttc gccggttgcg gcgtgccacg cggtgcatgt
    25561 atcgagggcc ttgttcccgc agagtcgcag tgaggctgtg aggcttccgt atgggctcga
    25621 tctcgtccgc ggcggccgcg acctcttcca ccggctgctg gtcgtcctct tcctccgctt
    25681 ccacgtccgc tgcggcatgg gagccttcgt cttcgctctc cagctcgtct tcttccggtt
    25741 ccgggccgga ccgcgcctcg gcgtccattt gagcttccag aatcatctcc cggatctcgc
    25801 tgtcgtcaaa ttcctcctgt tcgtcgagga cttcggccgc gtccgcactc tcgtcttcca
    25861 caacggtggc ggcgtcttct tcttctgttt cttccgcttc gctttccagt aggggttcgg
    25921 cctgttcttc ctcttcggac ggcatggctt cttcggcgac gtcgtccgcg gcagccgttt
    25981 cttctggctg gtcctcgatg gccgtcactt caccatcgaa atcccccaga agcgcttcga
    26041 catggctgga gggcgcttga tcgggagtct cctcttcggt tccggcaggt tcggacggtt
    26101 ctggtccgcg atatttggcg agtgattcgc ccggcaaaac cgcgaaatcc agctccgcaa
    26161 cggcaggcgt ttcctggatc tcctggtcct cttccgctgg ggcagtctcg gtcgcatact
    26221 tcgattccgg gaatcctcgt ccccggttgc gcctccgccg ggaccggcgg cttcgccggt
    26281 cgcgtccttc cggctcgtcg gttcgctccg gcgtagctgc cgcgggctca gcgatcaccg
    26341 gtggtgccgg cggtggaacc ttcacggaag cgacggccag cgacggacta tgtgccttta
    26401 cgggcttctc ttccgggatg atcctgtcgt attcgtcgct ttcttcgaag aagtccgaca
    26461 cgtacaaaaa cgtgtcccgc tcgagcccga ggtcgacgaa agccgactgc attccaggca
    26521 ggacgcgcgt gacgcgcccc ttgtgaatgc tgccggccag cgaatattcg tgcgaacgtt
    26581 ggaagaaaat ctcgaccagt tggtcgtctt cgagcaccgc cactctggtt tcgtggcgat
    26641 tggatgaaat cacaagctct ttggccatgc caattatctc cgtctgcttt acggccgggt
    26701 tggagggcta gcgcctccgg cgccggtccg ggtttggccg cggcgagtgc atttggcgtg
    26761 agggagagga gaacggatgc ttttctccgg cttcgatggc tgctgcgccg gtgatttttc
    26821 agtctactat cgggctgccc cgtttggccg cccctatctt tcaggtgtgg cgtatatacg
    26881 ccagaatcga gttctacggg cgggccaagg agcgtttccc gccctggctg cgcaacccga
    26941 caagccttgt tccccggcag tgggacctgt aactctgccc cgttacctgc cgcggctggc
    27001 ttcctgaacc tgcttaataa accgaatccg ctcttccccg agaacctcgg gacctaacgc
    27061 ctcaaacgcg ttgttaattc gtaagtctgc ggagccggac actatgaacc agccccagca
    27121 acataaaggt cgaaagtgta ttcgagccgc cggcactcat caacggcagc ggtattcctg
    27181 caaccggcat gcggcccacc accattccca cattcacaag aacgtgaaaa agcagcatcg
    27241 ccgcggctcc catacagata tacatgccgg agcggtctac ggccgattgg gcgttttgaa
    27301 cgatctgtaa aatcagcatc aaatacaacc ccaaccctac cagcactccg acaaatccgt
    27361 gttcttcagc gaaagccgaa aaaataaaat ccgtgtgcgg gacgggacga aaccgcagtt
    27421 gcgtctgtgt tccctgcgtc actcccaagc cccaaatgcc cccgttccca accgcgatcc
    27481 gcgactggat cacctggtat cccgaaccac gggggtcctc atccggatcc ataaacgtag
    27541 ccagccgcgc cttctgatag tccctgagaa aatgccatcc aatgggcaga accatcagga
    27601 aaatcagcgc gatcgccacc atgtactgcc agcgcagccc ggccagaaat actccgacta
    27661 ccagggcggg cacataggtc agacttgttc ccaaatcggg ctgcctcatc accagcagca
    27721 tgggaacacc caccaacccg ccaagtttca acaggtcgat gacgtccaac ctgtctttcc
    27781 ggacatcggt gatataccgg gctatcagca atattatcac cagcttcacg aattccgaga
    27841 cctgaagatg aaaacc
//
LOCUS       DASTMK010000515        13303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_250412, whole genome shotgun sequence.
ACCESSION   DASTMK010000515 DASTMK010000000
VERSION     DASTMK010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13303
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250412"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VFQ79_21370"
     CDS             <1..1192
                     /locus_tag="VFQ79_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142285.1"
                     /translation="LVLAYLGGHRSVQRFEQRFNIAHLATAGLPFLFLGLIAAQPGIG
                     ILSPEVLKEIAPLLSLGLGWIGFAVGSRFNARALDDLPPGTSAAVFLTTAFPIFAIFV
                     VCAVVLAASGQSVAESGYLRDALLLGTAGAMAARKSPRFARKMFPAERPIGRLARIVE
                     LQQLAGVFGLMMVSAYFRPHGPAVAWQLPGTAWLFIMLGIGTTMGVLVYATLTKINKD
                     PQFTVALLGAIAFTAGIASFLRLSPVAVCFIAGAIIVNLGGTWKEQVRTVLRHLERPV
                     YFVFLVIAGALWHPSEWQGWVLMVIFVTVRFASKWLSVELLRKFWVRDLAFRERRSLT
                     ASPVGALSIAIVVSAQDLYSGPSVPWIVTAVIGGAVVMEAVLQLSVRRMGPAKKPAPA
                     ALET"
     gene            1350..2759
                     /gene="glnA"
                     /locus_tag="VFQ79_21375"
     CDS             1350..2759
                     /gene="glnA"
                     /locus_tag="VFQ79_21375"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU0142286.1"
                     /translation="MSPKEVIEFAKKNEASQFDLRFTDIPGLSQHISFPIQQLDESRF
                     EEGFGIDGSSIRGWAVIHESDMLLIPDGTTAFMDPFSETPTLVMTCDVIDPITRQHYE
                     RDPRWIARKAEMYLKNSGMADEAFFGAEAEFFIFDNISFDQNQHSGYYYIDAEEGRWN
                     SGRREDNLGYRPRYKEGYFPTPPTDHYQDLRAEMVETMLRCGLNIECHHHEVATGGQC
                     EIDQKFDTLLKSADNMMVYKYCVRNVAYQYGKTVTFMPKPIFGDNGSGMHCHQSLWKG
                     GNPLFAGDCYAGLSQMALWYIGGLLKHARALSAIIAPTTNSYKRLVPGYEAPVNLAYS
                     RRNRSAAVRIPMYSASPKSKRVEFRPPDPSANPYLSFAALLMAGLDGILNKVDPGEPL
                     DKDIYDLSPEEMKDVPAMPGSLDEALTCLEDDHSFLLKGDVFTDELIETYVGYKRKAE
                     VDAVRLRPHPYEFAMYYDI"
     gene            complement(2959..4524)
                     /locus_tag="VFQ79_21380"
     CDS             complement(2959..4524)
                     /locus_tag="VFQ79_21380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0142287.1"
                     /translation="MEILIAGTSAYDDPAAEEWKPDLRLTVSFLRRHGADAGYVYVAS
                     PQEGGLSSLVEAAHLKAIFLELSEENRKPVLLAVRELGQRFPEIPVLLGGIPATLDAE
                     SVFDQCEQATFLVEGERELTLLETVERLRGHEPLDGIPGLRTREDRNPARPLLPDLDV
                     LGSIVYDGLPEMLSSYSAGDRTAFLRSSRGCYGTCSFCGIPGFYRRSPGAPWRGRSPK
                     VVVDEIEGLISTFGIRRFVFEDDDFMGPGVAGRERARGIASEIARRKLRIEYFLCCRL
                     NDVDAATMSALKASGLSGIGVSVESANQESLDLLKKGIKATAIYPKLRLLEELQIRCE
                     VNMIFFDPRVTLAGVRKNLELLDYVRRSEYLSYSDAFPFTELQAFPWSPVSRSLRAEA
                     LLEGDGPMCRFKDPLVSELAGLVRRLQKRMPIVFKKRLLFEKADSAFPHDAATAILRV
                     AAGMRHWAGLTVMPRFVAEACDILEKDPDDVTRQFVLLEERFDEAIEFLRILEDRIAG
                     LSGMESERPTMIR"
     gene            complement(4532..6103)
                     /locus_tag="VFQ79_21385"
     CDS             complement(4532..6103)
                     /locus_tag="VFQ79_21385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0142288.1"
                     /translation="MQPAADIDVLFADIPWDFSFPESSWCLGYRYMIASLRRHGFSAR
                     ILHPPSIRDGPVRERMIQQIIGSNARILGFTTYDVQLGLLLEFIKDLRRRGLRSHVTL
                     GGLCASAAAEALMTGYEAIDSVVFGEGETSIVELASQIIRGTKPSPIPGVYRRDGRHV
                     VTGGPRPLVEDLDSLTPPVLDNFADPECHAPLRLTNGCVPVTASRGCYGRCSFCCVQK
                     FYRSSPGKTWRGRGAAAVAEEISRVTAATGCRRITFVDENFMGPGAEGRTHAVEVARE
                     IKRWAPAIQFNFGCRANDVDRATMEELKSAGLAAVTLGIESMSAEALRLFNKGTTPAV
                     NYRALAILDELELFTEITFIFFHPLSTLREVRENLRLIEYVRQSKYLYFNNRQPFSAY
                     IPFFGTDLAAQFERKALAERTLYDFRTSYADPRVAFIAQAVLAVPSDALLNLRSLLPE
                     GRSPELTRMREALLGQYVYLNMVRIPELVKRMCDGFDQGEDTSGSGVCAAAAELQAEA
                     RRIETLTARFLAHVA"
     gene            complement(6087..7484)
                     /locus_tag="VFQ79_21390"
     CDS             complement(6087..7484)
                     /locus_tag="VFQ79_21390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEU0142289.1"
                     /translation="MHVARRIRVFDTGSNRHLLCNALTGEILVLSDAGLRLLDALARG
                     EPAACDSSVVEQWKRKSILFDAPEEEERQFVELCRKSWIEFQRSAPRQYTFVVNTHCN
                     FECGYCFEKAYDLPTRTLSIEQVDAAFAVLDRFSKNRRQTGGAQYELFGGEPLLPGSR
                     HIVDYLLARIGDRGGKASIQTNGYHLSSFVDLIAAHRNSVSQVQVTLDGPRNIHDRRR
                     VRKGGQPTFDQVVSGIDALTRKELPLRINVRMNVDRRNVDYLADMAAVYDEKGWTKNP
                     HFAFVAAPVDNRCGKLSGPDALMGWHELFERVFPLSTDLGGGPFDLSVFKTASYFRYY
                     VQAARESLAMGKHFAPEFIPKVLYCEAAGLKLFAFHPDGRIYPCPEAIGTEALAIGVY
                     HPAFHIDRGRARRWRHQTILNRARCRTCDISTFCGGGCVLTALMANGSMSEPVCENAA
                     DVMQCYFEKIGATSG"
     gene            complement(7494..8456)
                     /locus_tag="VFQ79_21395"
     CDS             complement(7494..8456)
                     /locus_tag="VFQ79_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142290.1"
                     /translation="MNSIPDELRIEDTSAVERQFLAGLAGLAPELQDAGGTLFRRIVT
                     PNWALEWGMPRWLGETFDLSEHVTGELVMANAYMLAYIRITDDLADGDAPGSSVRLAV
                     SIYHLWMRRYLRLFDEQRVQATRKFWAYFDECMMRWLNATMGAAQPVPPFGSYTEADY
                     LHLADRGATLKIVCAAACLLAGRERDLPLLGSATDDIMAGVVLLDDRFDWRTDLDADR
                     YNAFVAYCSDLPQIEEYRDANRRAVLEEIHFGKAAQPYFTVIAERAEHARRKAEAAGC
                     RLLARFAEWYAEEAVGCGVWLRNEAASRMHDFLVQHSVPVEPAS"
     gene            complement(8507..8779)
                     /locus_tag="VFQ79_21400"
     CDS             complement(8507..8779)
                     /locus_tag="VFQ79_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142291.1"
                     /translation="MSKERVFEDVRKILAYDEAVLKLQQAGLAEAFVTAVEKDPELKA
                     SVQKLAPNLTGAASPGWSCCVTVSNPLRSPGEAVINPAIVTQPIKK"
     gene            complement(8772..8960)
                     /locus_tag="VFQ79_21405"
     CDS             complement(8772..8960)
                     /locus_tag="VFQ79_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142292.1"
                     /translation="MKEVLEFAVVEKAIGVLRSAPWKKSEALALMEDILSAQLRRLLM
                     PVGTDISPDLTKGAMQYV"
     gene            complement(8963..10339)
                     /locus_tag="VFQ79_21410"
     CDS             complement(8963..10339)
                     /locus_tag="VFQ79_21410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0142293.1"
                     /translation="MPRSALTDILFVDLPFNSYELGRRFKSVWSYKQFLSAHELHLGF
                     RYMTAYLRHRGYQAGILYPSPSNGLTSKSALIRAIEQARPLLLGLTSYEGSLRESLQF
                     VQRVKARGNSPVICMGGHLATFSYEEILRDFPELVDVIVLGDGEETIAELAEAVREGR
                     GFEKIPGIAYSLNGAVVKTPQRPIQQNIDSLPFPALDTDDWQDRSVPLFLTSSRGCYG
                     RCSFCRSSHFGERWRAREPRNVADEIERAVERGVTTFEMVDDNFLGPGRAGRERALAV
                     ASEILRRGLRIRFHISCRVNDVDESTMRALKEAGLISVSLGVESGVQRILDTFNKNIK
                     VEQSLAALDILHKLDLPALVYIIFFDRYTTLEEAGENIRFLEHIRSLGNVRFEEILFR
                     KLIPISGTDLFERIRADGLLRGDYLSGHRFAFRDRRVSWLADFLETIDLRFERMMQRE
                     DFRRIGGL"
     gene            complement(10475..10954)
                     /gene="msrA"
                     /locus_tag="VFQ79_21415"
     CDS             complement(10475..10954)
                     /gene="msrA"
                     /locus_tag="VFQ79_21415"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0142294.1"
                     /translation="MATENATFGAGCFWGVEAAFRETKGVKNTVVGYAGGHTENPTYQ
                     DVCTDRTGHAEVVQVEYDPAEVSYGDLLNVFWSVHDPTTLNRQGPDVGTQYRSVIFFH
                     SPEQQRAAQASKEERERSGLFKRPIVTQIEPAPVFYPAEDYHQQYLEKRGLAHCHIR"
     gene            complement(10973..12082)
                     /locus_tag="VFQ79_21420"
     CDS             complement(10973..12082)
                     /locus_tag="VFQ79_21420"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0142295.1"
                     /translation="MIAAIAAIAAAAIAYQVASLLAAWRHLRAPASAGDYTPAISVLK
                     PVRGLDPDFYEAIRSHAAQDYPAFEILFGTADESDPALSSIRRLAADHLGVPMRVIVS
                     STSAPNQKAGILCDLAAAARHSVYVVSDSDVKVPQDWLRGIVAPLADSRTGVVTCLYR
                     PVARTAAGVWEALGVATDFAPSILVARWIGVREFGLGSTLVFRAADLERAGGFPAIAG
                     YLADDYQLSKRISGLGLETVLSRTVVETSLGEDGWTGVWKHQVRWARTIRVSRGGGYA
                     GLPVTHAGVWVLASAIAGLWPLAFVLLLLRIASGALTGIGVLGSGIVKRWFLAAPLWD
                     LWAFAIWIAGLLGTTVEWRGRKLELSREGRIKNVD"
     gene            complement(12079..12444)
                     /locus_tag="VFQ79_21425"
     CDS             complement(12079..12444)
                     /locus_tag="VFQ79_21425"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0142296.1"
                     /translation="MTKWVLVMLIVLSTTAGDLLLSREMKRHGEIDNFHPSRIGRLVA
                     ALVKRKFLVLGIAFMAFSFFAFMRLLTVADLSFAVPATAASYATETLLARYILKERVD
                     PVRWAGACLVMCGVALLAL"
     gene            complement(12441..12863)
                     /locus_tag="VFQ79_21430"
     CDS             complement(12441..12863)
                     /locus_tag="VFQ79_21430"
                     /inference="COORDINATES: protein motif:HMM:NF017468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0142297.1"
                     /translation="MDASHTKLRLKTCVFSAVVILTNVLGNFFLSLGLRGGTLASESP
                     LAYIKLMFSPWVAAGIILLSVWMLSRMTLLSWADLSFVLPVTSLGYVVSALMARIFLK
                     EQITGQRWFGTLLIVAGTALVGMTGPRTTTPSSKPDTA"
     gene            complement(12871..>13303)
                     /locus_tag="VFQ79_21435"
     CDS             complement(12871..>13303)
                     /locus_tag="VFQ79_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEU0142298.1"
                     /translation="VGLPGETRESIRSTIDFAKRLDVETIQVSIAHPYPGTEFYDHVK
                     QNGLITLDSMTDEAGHQLPNIVYPGLDRAELVDWVEKFYGEYYFRPRVAWRVVRKAIF
                     HGDERRRLYKEAREYLALRSKRKKFVKDQRTSAGAMAGAGD"
BASE COUNT         2686 a         4056 c         3922 g         2639 t
ORIGIN      
        1 gctggtattg gcctatcttg gcgggcaccg tagcgtccag cgtttcgagc agcggttcaa
       61 catcgcacac ctggcgacag cagggctccc attccttttt ctcggcctga ttgcggcgca
      121 gcccggcatt ggcattctgt ctccggaagt tctcaaagag atcgcgccgc tgctgtcact
      181 ggggcttggc tggatcggct ttgcggtggg ttcgcgtttc aatgccaggg cactcgacga
      241 cttgccgccg ggtaccagcg ccgccgtttt tctcacgacg gcatttccga ttttcgccat
      301 ctttgtagtg tgcgcggtgg tgctggccgc gtcgggccag tctgtcgcgg agtccggata
      361 tctgcgtgac gcgctcctgc tcggcactgc cggagcgatg gcggcacgca aatctccccg
      421 gtttgcgcgg aagatgtttc cggcggagcg tcctatcggc aggctcgcgc ggattgtcga
      481 gttgcagcaa ctggcgggag ttttcggact gatgatggtc agcgcctatt tccgcccgca
      541 tggcccggcg gtggcatggc agcttcccgg tacggcgtgg ctgtttatca tgcttggcat
      601 cggcaccacg atgggcgtgt tggtttacgc gactctaacg aagatcaaca aggatccgca
      661 gttcaccgtg gcgctgctcg gcgcaatcgc ttttacggcg ggcattgcca gctttctgcg
      721 gctgtctccg gttgcggtct gctttatcgc cggcgcgatc atcgtgaatc tcgggggtac
      781 gtggaaggaa caggtgcgca ccgttttgcg ccacctggag cgcccggttt acttcgtatt
      841 tctcgtaatc gccggggcgt tgtggcaccc gtctgaatgg cagggctggg tattgatggt
      901 gatctttgtg accgtccgct ttgcgtcgaa atggctatcg gtcgaattgc tgcgaaagtt
      961 ctgggtgcgc gatctggcgt tccgggagcg ccgcagcctt accgcgtcgc cggtgggtgc
     1021 gctttccatt gccatcgtcg tgagcgcgca agacctctat tccggtccct ccgtcccgtg
     1081 gattgttacc gccgtcatcg gaggagccgt ggtcatggaa gcggtactgc agctctcggt
     1141 tcgccggatg ggacctgcca aaaagccggc tcctgcagcg ttagagacat gagcgggcga
     1201 atgcggaatt tgtgatcctc cccataagta atttcgattg accgctttct tcgctattcg
     1261 ttaacctgac aatttatcaa gcaggaaccg atgcacaata ccagagcaag ctgcggcttg
     1321 ccgcgagtac gggcatccac ggaggttcaa tgagtcccaa agaagtcatc gaatttgcca
     1381 agaaaaacga agcgagtcaa tttgacctgc gctttaccga catccccggg ttatcgcaac
     1441 atatctcttt tcctatacag caacttgacg aaagccggtt tgaagaaggc ttcggaatcg
     1501 acggttcgag catacggggc tgggccgtca tccatgagag cgatatgctg ctcatccccg
     1561 atggcaccac cgcgttcatg gatccttttt ccgagactcc cacactggtg atgacgtgcg
     1621 acgtgatcga ccccatcacg cggcaacact acgagcggga tccgcgctgg atcgcccgga
     1681 aagccgagat gtacctgaag aacagcggca tggcggacga ggcttttttt ggcgcggaag
     1741 cggaattctt catcttcgat aacatcagct tcgatcagaa ccagcactcg ggctattact
     1801 acatcgacgc ggaagaggga cgctggaatt ccggacgcag ggaagacaat ctcggttacc
     1861 gtcctcgcta caaggaaggc tatttcccga cgcctccaac ggatcactac caggatttac
     1921 gggctgagat ggtggagacg atgctccggt gcggtctgaa catcgaatgc caccatcacg
     1981 aggtcgctac gggcggccag tgcgagatcg atcagaaatt cgacaccttg ttgaagtccg
     2041 ccgacaacat gatggtgtac aagtactgcg tacgcaacgt cgcgtatcag tatggcaaaa
     2101 ccgtcacgtt catgccgaag ccgattttcg gcgacaacgg cagcggaatg cactgccacc
     2161 agagcctgtg gaagggcggc aatccgctgt ttgccggcga ctgttacgcc gggttgagcc
     2221 agatggcctt gtggtacatt ggcggcctgc tgaaacacgc ccgcgcgctt tcggccatca
     2281 ttgctcccac caccaacagc tacaagcggc tggtcccggg ctatgaagcg cctgtgaatc
     2341 tggcctactc acgccggaac cggtcggccg ccgtccggat tccgatgtat tcggcgagtc
     2401 cgaaatcgaa gcgcgtcgag ttccgcccgc cggatccctc ggccaacccc tacctgtcgt
     2461 ttgcggccct cctgatggca ggtctcgacg gcatcctgaa caaagtggat ccgggcgaac
     2521 ccctggacaa ggacatctac gatctttcac ccgaggaaat gaaagatgtg cccgcgatgc
     2581 cgggttcgct ggatgaggcg ttgacgtgcc tcgaagacga tcactccttc ctgctgaagg
     2641 gcgatgtctt caccgatgaa ctgatcgaga cctatgtcgg ctataagcgc aaggcggagg
     2701 tggacgctgt aaggctgcgg ccgcacccgt acgagtttgc gatgtattac gacatctgag
     2761 gacacatatt ttcgcggggc cgctccctca cggtcacggc tcagaaacgg agccgcgcgc
     2821 gtaagcaagc ggatagcggt atggcctctt aacgggcagc gtggcgaatg cggtcctgcg
     2881 tgagcgtcaa cgggtactgt ttcgccgggg aaatcgaaca ggagaacgaa caaggccgac
     2941 caaaacgctg cccgcgtgct agcgtatcat agtcggcctt tcgctttcca tgccggatag
     3001 cccggcaatg cgatcctcca ggattcgaag aaactcgata gcctcatcga accgctcttc
     3061 gagaagaaca aactgccttg tcacatcgtc cggatccttt tccaggatgt cgcatgcctc
     3121 ggccacgaag cgcggcatca ccgtaagacc ggcccagtgt ctcatgcccg cagctacgcg
     3181 caggatggct gtagcggcgt catggggaaa ggctgagtcc gctttttcaa acagcaggcg
     3241 cttcttgaag acaatcggca tgcgtttttg gagccggcgc accaggccgg cgagctccga
     3301 gacgagtggg tccttgaacc ggcacatcgg tccgtccccc tccagcaacg cttccgcacg
     3361 caggctcctg ctgaccggcg accagggaaa cgcctgaagt tcggtgaaag ggaacgcgtc
     3421 ggagtagctg agatactccg aacgccggac ataatccagc agttccagat ttttccggac
     3481 gccggccagg gtcactcgcg ggtcgaagaa aatcatgttg acctcgcacc ggatttgcag
     3541 ttcctcgagc aggcgcagct tcggataaat cgcggtggct ttgatgcctt tcttcaggag
     3601 atcgagcgat tcctggttcg ccgattcgac cgaaacgccg atgccggaca gccccgaggc
     3661 ctttaacgcc gacatggtag ccgcgtctac gtcgttcagc cggcagcaca gaaaatattc
     3721 gatacgcaat ttgcgccgcg cgatctcgct ggcgattcca cgcgcccgct cgcggcctgc
     3781 cacaccggga cccatgaaat cgtcgtcctc aaaaacgaag cgccggattc cgaacgtact
     3841 gatgagtcct tcgatctcgt ccactaccac tttcggactg cgcccgcgcc agggagcccc
     3901 cggcgagcgg cgatagaagc cgggaatacc gcagaaactg cacgtcccat agcaaccgcg
     3961 gctgctacgc aggaacgccg tgcggtcgcc ggcgctgtag ctggagagca tctccggcag
     4021 cccgtcgtaa acgatggagc cgagaacatc caggtcgggc agcagcggcc tggccggatt
     4081 ccggtcttcg cgcgttcgca gtcccgggat accgtcgagg ggttcgtgcc cgcgcagccg
     4141 ttcaaccgtt tcgaggagtg ttagctcccg ctctccttca acgaggaagg ttgcctgctc
     4201 gcattgatcg aagacacttt ccgcgtccag cgtcgctgga atcccgccga gcagcaccgg
     4261 tatctccgga aatctctgtc cgagctccct aaccgccagc agaaccggct tgcggttctc
     4321 ttcggacaac tcaagaaaga tcgcctttag gtgtgccgcc tcaaccagag acgatagtcc
     4381 gccttcctgc ggcgatgcga cgtaaacgta tcccgcatcg gcaccgtgcc gcctcagaaa
     4441 cgaaacggtg aggcgaagat ccggcttcca ctcttcggcg gccggatcgt cgtatgcgga
     4501 ggtgcctgca atcaggatct ccatatgccg ctcatgccac gtgcgccagg aaacgcgcgg
     4561 tcaaagtttc gatgcggcgg gcttcggcct gcaactctgc cgctgccgcg cacactccgc
     4621 tgccggaggt gtcttccccc tggtcgaacc cgtcgcacat gcgcttcacc agttcgggaa
     4681 ttctcaccat gttgagatac acatattggc cgagcagagc ttcccgcatc cgggtcaact
     4741 ccgggctgcg gccttcgggc agcagactgc gcaggttcag cagcgcatcc gacggcacgg
     4801 cgagaaccgc ctgggcgatg aatgcgacgc gaggatcggc gtagctggtc cggaaatcgt
     4861 acagcgtcct ttccgccagc gccttacgtt caaactgcgc ggccaggtcc gtcccgaaga
     4921 acggaatgta ggcggagaac ggctgccggt tgttgaagta gaggtatttc gactgccgga
     4981 catattcgat gagacggaga ttctcccgaa cttcgcgcaa ggtcgaaagg ggatgaaaaa
     5041 aaatgaacgt aatttccgtg aaaagctcca gctcatcgag gatcgccagc gccctgtaat
     5101 tcacggcggg tgtggttccc ttgttgaaca gccgcagtgc ctccgccgac atcgattcaa
     5161 ttcccagcgt gacggcggcg agcccggcgc tcttcaattc ttccattgtg gcgcggtcga
     5221 cgtcgtttgc ccggcatcca aagttgaact ggatcgccgg cgcccaccgt ttgatttccc
     5281 gcgccacctc gacagcgtgt gtccgccctt ccgcgcccgg acccatgaag ttctcgtcga
     5341 cgaatgtaat gcggcggcag ccggttgcgg ctgtcactct cgagatttct tcggcgaccg
     5401 ccgccgctcc tcggcccctc caggtcttgc cggggctcga ccggtagaat ttctgcacac
     5461 aacaaaaact gcatcggcca taacatcctc gcgaagcggt caccgggaca cagccgtttg
     5521 tcaggcgcag cggagcgtgg cactccggat cggcaaaatt gtccagcacg ggcggcgtca
     5581 gcgaatcgag atcctccacc agtggtcgcg gtccgccggt tacgacgtga cggccatctc
     5641 ttcggtacac tcccggaatc ggagaaggct tggttccgcg gatgatctgc gaggccagtt
     5701 caacgataga tgtttcgccc tcgccgaaga cgacggaatc gatcgcttca tagccagtca
     5761 tcaaagcttc cgctgccgcc gacgcgcaaa ggcccccgag cgtcacgtgg gagcgcagcc
     5821 cccgccgccg caaatctttg atgaactcca gcagtaggcc cagttggacg tcgtaggtgg
     5881 tgaagccgag aatgcgcgca ttcgagccga tgatctgctg aatcatgcgc tcccgaaccg
     5941 gcccgtcccg aatcgacggc gggtgcagaa tccgcgccga aaatccgtgg cggcgcagcg
     6001 atgcgatcat gtaccggtag cccagacacc aactgctctc cgggaaggaa aagtcccagg
     6061 gaatgtcggc gaacaggacg tcgatatcag ccgctggttg caccgatttt ctcgaagtag
     6121 cactgcataa cgtccgcggc gttctcgcac accggttcgg acatgctgcc gtttgccatc
     6181 aatgcggtca gcacgcagcc tccaccgcag aaagtagaga tatcgcacgt ccggcagcgc
     6241 gcgcggttca gaatggtctg atgtctccac cgccgcgcac gtccgcggtc gatgtggaac
     6301 gccgggtgat aaacgccgat cgcaagggct tccgtcccga ttgcttccgg acagggataa
     6361 atcctgccgt cgggatggaa ggcgaacagt ttcaaccccg cagcctcgca atacagaact
     6421 ttcggtatga actccggagc gaaatgtttc cccatggcga gcgattcccg tgcggcttga
     6481 acataatacc ggaaatagct cgcggtcttg aataccgata agtcgaacgg accgcctccc
     6541 aggtccgtag agagcggaaa cacccgctcg aacaactcgt gccagcccat cagggcatcc
     6601 ggaccggaaa gcttcccgca ccggttatcg acgggagccg ccacgaacgc gaagtgcggg
     6661 tttttcgtcc agcctttctc gtcgtacacg gcggccatgt ccgccaggta atcgacattg
     6721 cgccggtcta cgttcatccg cacattgatt cgaagcggca gctctttgcg tgtgagcgca
     6781 tcgatgccgg agacaacctg gtcgaatgtc ggctgcccgc cctttcgcac gcggcgcctg
     6841 tcgtggatgt tgcgcggccc gtcgagcgtc acctgcactt gcgaaacact gttccggtgt
     6901 gcagcgatca ggtcgacgaa gctggacagg tggtagccgt tggtctgtat tgacgccttt
     6961 cctcccctgt cgccaatgcg cgccagcagg tagtcgacaa tgtggcgaga gccgggcaga
     7021 aggggttcgc cgccgaacaa ttcgtactga gcacccccgg tttgccggcg gtttttcgag
     7081 aaccggtcaa ggacggcaaa cgcagcgtcc acttgttcaa tcgaaagcgt acgcgtggga
     7141 agatcgtaag ccttttcgaa gcagtaacca cactcgaaat tgcagtgggt gttcacgacg
     7201 aacgtatact gccttggcgc ggagcgctgg aactcgatcc acgatttccg gcaaagctcc
     7261 acgaattgcc gctcttcctc ctccggcgca tcgaagagga tgctctttct tttccactgt
     7321 tcgacgacgc tgctatcaca cgcggccggt tctccccgcg cgagcgcgtc gagcagccgc
     7381 agacccgcat cgctcaatac caatatctcg cccgtgaggg cgttacagag aagatgcctg
     7441 ttcgaaccgg tatcgaacac tcggatgcgt ctggcaacgt gcataccgtc tcctcacgaa
     7501 gccggttcca ccggaacgga atgctggacg agaaaatcgt gcatccgcga cgcagcctcg
     7561 ttccgcagcc acaccccgca cccgacggcc tcttccgcgt accattcagc gaagcgggcc
     7621 agaaggcggc agcccgccgc ttctgctttc cgccgggcgt gctccgcacg ttctgcgatg
     7681 acggtgaaat agggctgggc ggctttgccg aagtgaatct cctcaagcac cgcgcgccgg
     7741 tttgcgtccc ggtattcttc tatctgcggg agatctgagc aataagcgac gaacgcgtta
     7801 tacctgtccg catccagatc cgtacgccag tcgaatctgt catccagaag aaccacgcca
     7861 gccatgatgt cgtcggtcgc cgagccgagc agcggaaggt cgcgctcccg gcccgcgagg
     7921 aggcaggctg cggcgcatac gattttcagc gtggcgccac ggtcggcgag gtgcaggtag
     7981 tccgcctcgg tgtacgatcc gaatggaggc acgggctgcg cggcccccat tgtcgcgttg
     8041 agccagcgca tcatgcattc atcgaaatag gcccaaaact tgcgtgttgc ttgcacacgt
     8101 tgctcatcga acagccgcaa gtaccgcctc atccataagt ggtagatcga aacagcgagc
     8161 cgcacgctgc tgccgggtgc gtcgccgtcg gccaggtcgt cggtgatgcg gatgtacgcc
     8221 agcatgtacg catttgccat caccagctcg ccggtgacgt gttcggacag gtcgaatgtc
     8281 tctcccagcc agcgcggcat gccccattcg agcgcccagt ttggagtaac gatccggcga
     8341 aacaaggtgc cgccagcgtc ctggagttcg ggcgcgagcc cggcgagccc ggccagaaac
     8401 tgccgttcca ccgccgaggt atcttcaatg cgtaactcgt ccgggataga gttcacctga
     8461 gaataagtgg agccggagtg cggctccacc ctccgatcat tgctgcttat tttttgatcg
     8521 gctgcgtcac gatcgccggg ttgatgacag cttctcccgg agatcgaagc gggttgctta
     8581 cggttacaca gcacgaccag ccggggctcg ccgcaccggt aaggtttggc gccagttttt
     8641 gtacggacgc ctttagttcg ggatcctttt ctacggcggt tacaaacgct tccgcgagtc
     8701 cggcttgctg aagtttcaaa acagcttcat cgtaggccag aattttgcgg acgtcttcga
     8761 acacacgttc tttagacata ttgcattgct cctttcgtta aatcagggga aatatccgtc
     8821 cccacgggca tcaacagacg gcgtaactgc gcactcaaga tgtcttccat cagcgccagg
     8881 gcttcgctct tcttccaggg cgcggaccgc aggacgccga tggccttctc gacgacggcg
     8941 aactcgagga cttctttcac gcctagagtc ctccaatccg ccggaaatct tcccgctgca
     9001 tcattcgttc gaagcggaga tcgatggtct ccaggaagtc ggccagccag gagacacgcc
     9061 ggtcccggaa cgcgaagcga tgaccgctca aatagtcgcc acgcaggagc ccgtcggcgc
     9121 gaatccgttc gaaaagatcc gtgccgctga tcggaatcag tttgcgaaaa agaatctctt
     9181 cgaagcgaac atttccgagt gagcggatgt gttcgaggaa acgaatattc tccccggcct
     9241 cttcgagcgt cgtatagcga tcgaagaaga tgatgtaaac aagagcgggc aggtccagct
     9301 tgtgcaggat atcgagggcc gcaagactct gctcgacttt gatgtttttg ttgaacgtgt
     9361 cgagaatccg ctgtacgccg gattcgaccc ccaggctcac cgagatcaga ccggcctctt
     9421 tgagagcgcg catggtggat tcatcgacgt cgttcacacg gcaggagatg tgaaagcgga
     9481 ttcgcagccc tcttctcagg atctcggaag ccacggccag cgcgcgttct ctacccgctc
     9541 tgcccggccc cagaaaattg tcatcgacca tctcgaaggt ggtcacgccg cgctccaccg
     9601 cccgctcgat ttcgtcggcg acattccggg gctcgcgcgc tctccatcgt tcgccgaagt
     9661 gcgaggaacg gcagaacgag caccgtccat agcatccgcg gctgcttgtc agaaacagcg
     9721 ggacggagcg gtcctgccaa tcgtccgtgt ccagagcggg aaacggcaag gagtctatgt
     9781 tctgttggat aggccgttgc ggcgttttca caacggcgcc gttcagggag tacgcgattc
     9841 cggggatctt ctcgaaaccg cgtccttccc tgacggcctc cgccaattcg gcaatcgttt
     9901 cttcgccatc gccgagcacg ataacgtcga caagctccgg gaaatcccgc aggatctcct
     9961 cgtaggaaaa agtcgcgagg tggcctccca tacagatcac cggggaattg ccccgggctt
    10021 tgacgcgctg cacgaactgg agcgattccc gtaaagagcc ttcgtaggaa gtgagtccga
    10081 gaagaagagg ccgggcctgc tcaatcgcgc ggatcagagc gcttttggac gtaaggccgt
    10141 tcgacggcga aggatagagt atgccggcct ggtagccccg gtgccgcaga taggccgtca
    10201 tataacggaa gcctaaatgc aactcatgcg cgctcagaaa ctgcttgtag gaccagaccg
    10261 acttgaagcg ccggccgagt tcgtaactgt tgaacggcag atcgacgaac agtatgtctg
    10321 tgagcgcaga acgcggcacg gccgcctccg tgacaggctt ttactgtaca gtgccgcgcc
    10381 tcggaattca agtcaaaaag acataaaaat ccattttaat aatcgtaacg aagatcgcat
    10441 tccaggacgg aaaaatcagt ggctgctctc gcggtcagcg gatgtggcag tgtgccaggc
    10501 cgcgcttttc gagatattgc tggtgatagt cttccgcagg atagaacacg ggtgccggtt
    10561 caatctgcgt aacgatggga cgcttgaaca gtcctgagcg ctcccgctcc tctttggatg
    10621 cctgcgcggc ccgctgctgt tcggggctgt gaaagaaaat caccgaccgg tattgcgtgc
    10681 ccacgtccgg cccctgacgg ttcaaagtag tagggtcgtg gacgctccag aacacgttca
    10741 gaagatcgcc gtaggagact tcggccggat cgtattccac ctggaccacc tccgcatggc
    10801 ccgtgcggtc tgtgcagacg tcctgataag tggggttttc ggtatgtccg cctgcatatc
    10861 ccacgacggt gttcttcacc cctttggttt cccggaaggc cgcctccaca ccccaaaagc
    10921 agcctgctcc aaatgttgca ttctcagtag ccatcccttt accctcctga tttcagtcta
    10981 cgtttttgat gcggccttcg cggctcaatt ccagtttccg gccgcgccat tccaccgtcg
    11041 tacccagcag accggctatc cagatcgcga aagcccaaag gtcccaaagc ggcgccgcga
    11101 gaaaccacct tttcacgatt ccggaaccga gcactccaat acccgtaagc gcaccggaag
    11161 ctatacgcag caacagcagc acgaacgcga gcggccatag gccggcaatg gcgctcgcca
    11221 gaacccagac tcctgcgtgc gttaccggaa ggcccgcgta gcctccgccc cttgatacgc
    11281 gaatggttcg cgcccagcga acctggtgtt tccagacgcc ggtccagccg tcttcgccaa
    11341 gcgaggtctc cacgacggtt cgggagagga cggtttcgag gcccaggccg ctgatgcgtt
    11401 tcgagagctg ataatcgtcg gccagatatc cggcaatggc cggaaatccc ccggcccttt
    11461 ccaggtcggc ggcgcggaag accagcgtcg atcccagacc gaattcccga acgccgatcc
    11521 atcgtgccac cagaatgctt ggcgcgaaat ccgtcgccac cccaagcgct tcccagacac
    11581 cggccgcggt ccgcgccacc ggccggtaga ggcacgtcac tacgccggtt ctgctgtcgg
    11641 caaggggcgc cacaatgccg cgcagccagt cttgggggac tttgacatcg ctgtcgctga
    11701 cgacgtaaac cgaatgccgc gcggcggccg cgagatcgca gaggatgcct gctttctgat
    11761 tcggtgcact cgtggaactg acgatgacgc gcatcggaac gccaaggtga tcggctgcca
    11821 ggcgccggat ggacgagagg gccgggtcgg attcgtccgc cgtaccaaac agaatctcga
    11881 acgccgggta atcctgcgcc gcgtgcgacc ggatcgcttc gtaaaaatcg ggatcgaggc
    11941 ctcgcaccgg cttcaatacg gagatcgctg gcgtgtaatc ccccgcggag gccggcgctc
    12001 gcagatgcct ccatgccgcg aggagggatg caacctggta ggcaatggcg gccgctgcaa
    12061 tcgcggcgat ggctgcaatc acagcgcgag cagggccacc ccgcacatca ccaggcaggc
    12121 gccggcccaa cggaccgggt ccactctttc ttttaaaata tacctggcaa gaagtgtttc
    12181 agtggcatag ctggccgccg tggcgggaac ggcgaagcta aggtcggcga cggtcagcag
    12241 tctcatgaac gcgaagaagg agaacgccat gaaggcaatt ccgagcacaa ggaacttccg
    12301 cttcaccagc gcggctacaa gacggccgat gcggctcggg tggaaattgt cgatctcccc
    12361 gtggcgcttc atttcacggg agagcagcaa gtcgccggcc gtcgtggaaa ggacgatcag
    12421 catgaccagc acccatttcg tcatgctgtg tccggcttgc tggaaggcgt cgtagtgcga
    12481 ggtcccgtca tgcccaccag ggcggttcca gctacaatga gcagcgttcc gaaccagcgc
    12541 tgtccggtaa tctgctcctt caggaatatc cgcgccatca acgccgacac gacatagccg
    12601 agagaggtga cggggagtac gaagctgagg tccgcccagc ttaacaatgt cattcgcgag
    12661 agcatccaga cggagagcag tatgattccg gctgctaccc aggggctgaa catcagcttg
    12721 atataagcca ggggcgattc gctggccagt gtgccgcccc ggagtccaag gctgaggaaa
    12781 aaattgccca acacattggt aagaatcacg acggcgctga agacgcaggt cttcaggcga
    12841 agtttggtat gtgaggcgtc catccgggaa tcagtctccc gcacccgcca ttgcgcccgc
    12901 ggatgtcctt tgatctttga cgaatttctt gcgtttggag cgcagcgcca ggtactcgcg
    12961 cgcttctttg tagagcctgc gccgctcgtc gccgtggaaa atggcctttc gcacgacgcg
    13021 ccaggctaca cgaggccgga agtagtattc gccgtagaac ttctcaaccc aatccaccag
    13081 ctcggcgcgg tcgaggccgg ggtaaacgat gttcggaagc tgatggccgg cctcatcggt
    13141 cattgagtcc aacgtgatca gcccgttttg tttcacgtgg tcgtagaatt cggtaccggg
    13201 gtagggatgc gcgatcgaca cctgtatggt ctccacatcc aggcgcttgg cgaaatcgat
    13261 ggtgctgcgg atgctctccc gcgtttctcc gggcaggccg act
//
LOCUS       DASTMK010000516         7624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_569695, whole genome shotgun sequence.
ACCESSION   DASTMK010000516 DASTMK010000000
VERSION     DASTMK010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569695"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFQ79_21440"
     CDS             complement(<1..300)
                     /locus_tag="VFQ79_21440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142299.1"
                     /translation="MKWVLLLVACSFSLPAATFYVTVAGLGGEPDYEQRFNDWARELD
                     KLVKTGNVNARAVTLRGEAATREAIRSTFGKIAQEASLSDDLVVTLIGHGSFDGED"
     gene            complement(297..2483)
                     /locus_tag="VFQ79_21445"
     CDS             complement(297..2483)
                     /locus_tag="VFQ79_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU0142300.1"
                     /translation="MWILPALIAVAAVLLAIAVWRRPAGERSPIRGSRKAAIWGLQTA
                     LAALVFLLLWQPAISIATLKPQQNVIAVVVDDSQSMAIPEEGGTRLSIAQSALGSGIL
                     EKLGERFQVRLYRLGSHLERIDKLQDLKASMPSTRINDALKQVVAESASLPIGAVLLL
                     SDGADNTAGIDLEMIKNVRSRRLPIHTVGIGREQFARDLEISDVQVAPRTLADSRLSA
                     YVSFRQRGYAGGKATLKVVENGKTLASQQVTLKPDGVQQTEAIVFNAGIAGAKNLQFV
                     VDALANEENGANNSLTRLVNVQSDRPRILYIEGEPRWEFKFIRRAMEEDRGLSLVSIL
                     RTTQNKIYRQGIDNPKELEDGFPSRVEDLFGYQAIILGGIEVTSFTSTQQELIRQFVD
                     RRGGGLRFLAGRSGLADGGYGTSPLADLLPVSLPNAKNTFHRDPATVELTPAGRDSLI
                     CRLEGDPASNADRWKKLPYLANFQEVGEPKAGAVVLAEFLPTSKGRFPLLVTQKYGMG
                     RTAVFASAGSWRWQMQQPLADRTHEMFWQQLLRWLATETPGRVLSSSPQPMLYDNGRL
                     QLRARVHDTSYLAMADATVEARIIGPENTSAVVTLSPDPLQNGDYTAEWVAERPGSYL
                     AEMLAKRGNEEIGRDVLMFRREDGVAEKFHAEQNRELLEMLASETGGRYYNPRNLSNI
                     ADEIQYSEAGIATRETRDLWNMPAVFLLALLLRASEWLLRRRWGAV"
     gene            complement(2579..3394)
                     /locus_tag="VFQ79_21450"
     CDS             complement(2579..3394)
                     /locus_tag="VFQ79_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEU0142301.1"
                     /translation="MRPRGSFIAIAIFFAALGAAFALQRSVLQQFLDREDDPIITPPD
                     ANEKTEWVFARLRYPSFRGSGMGGYWAMRGNWAIDYPKADRQFVQGVRRLTRIHARSV
                     EKVVDLDSDEIFDSPWVYVVEPGHWDLTDAQCAKLREYLLRGGFLMTDDFHGTFEWDV
                     FMASMSRVFPDRPVVDLENSDPIFHVLYDLDERFQVPGIVMFQTGRIYEYDGLEARWR
                     GIYDDRGRLMVAICHNMDLGDAWEWADHPLYPEKYASLAYRIGINYIIYSMTH"
     gene            3464..3691
                     /locus_tag="VFQ79_21455"
     CDS             3464..3691
                     /locus_tag="VFQ79_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142302.1"
                     /translation="MYCCDELQQLAGRELVRIGPVHTLTNGRILTEIDTEYFLVFGEE
                     RPTYAGINYCPFCGRALSRGLWNLEKKKQGR"
     gene            complement(3731..5512)
                     /locus_tag="VFQ79_21460"
     CDS             complement(3731..5512)
                     /locus_tag="VFQ79_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142303.1"
                     /translation="MRITSFATAALAVWPLVAAGAGAPLAFRTVDLQDARRQIDSYSQ
                     VDTRAAMWIWTDKYVYQPGEPITVRWTIKPNDDLYPYTLVAFHQNNQTGTRSYLPGND
                     TTPTDIFGNTVEQGFRIVRLPAAEKGVLAGEGGSVASTALTAPGELGMHTIVVQLRDY
                     TGTRVVKAAYFKFGVVSAIEDLPSSINENRTLTNDRAYRVQGIVAVRNNAVLTIEPGT
                     FIIGMPGSQPPSVLLVTTQGRLIANGTRSRPIIMTSSQPLGQRQRGDWGGLLLLGTAP
                     LNDPSGQLTIEGLPEMPETRYGGSNADHNCGTLRYVRVEFAGALLRPNEETNSFTWGG
                     CGKATTSEYLQAHYGLDDSFEWFGGNNDAKYLVGTYGADDYVDVQIGYNGRVQHVVAV
                     ANEDLSNRAVETDNYERDFAARPLGKLNLWNATFVGSAARGFDESDSPCLYFRRGAGG
                     STNNVICYNWTTRAFGGSDFSSIQPHIDSGDFTADGILMWDNGKESGRANSLDAQVQD
                     VFLPFASGGQQGRNFVVADPMLRRPLERSDPDFRPMLGSPVYRATWVQPPDDGFFDQT
                     ANYIGAFGDYDWTEEWTTFIQEQDLRP"
     gene            complement(5553..>7624)
                     /locus_tag="VFQ79_21465"
     CDS             complement(5553..>7624)
                     /locus_tag="VFQ79_21465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142304.1"
                     /translation="GLVQLRTVEFPVEKMLSVSVNYGFNTLSTFNRFNTYRGGSRDWT
                     GFDDGSRSLPGSIPPDQRLFVGNFNEQQFQQFGRAFSNNYEPEPVDSMRPSQTYSVAG
                     GNSWGRLGLVGALTFSNAPQRYPELRRFLVNSGGGRPQIFSDYPEFNTDTESVRIGGV
                     FNAAYRLSPGAKLIWRNTVTRDTDKEARFLEGLNGGNNNYIQDTRLRWVERGLLSSSI
                     EGDHAVARLGNSVFHWQFTYSYSTRNEPDLRETIYGRAADGARYEYLNLPESGMRFFN
                     GLNDRIYEPQGDWSKPFFKGSISGLFKVGFRATTRRRDFEGRRFRFFPVRAQTIDFTK
                     PANEVLGEANIRPDGFVVREITRGTDTYTASMDVYGGYGMIDLAIGRRWRFIGGARIE
                     DADVVVSTIDPLVPGSMPSVANLVNRDFLPAVNVIYALNGRQNLRFAYGRTVNRPDFR
                     ELSPFEFTNVVGGYSTVGNPELQRATIDNFDARWEWFLGGNQVIAAGYFYKSFTDPIE
                     QIYRPTASELRQSFLNVPEARNQGVEFEFRKNLGSFSGRLSPFAIQTNLTFVDSNVGI
                     PTDRFPQLTSRTRPLVGQSRLIYNVITEWVKPSWRSNARFYVNSVSRRITDVGTFQLP
                     DVYQERTTMLDFVYHVNLDETGKWILRFGAENLTDNHYRFTQADILVRAFRIGRTFTV
                     GTSYSFF"
BASE COUNT         1579 a         2277 c         2182 g         1586 t
ORIGIN      
        1 atcttcaccg tcgaagctgc catgtccaat gagcgttacg acgagatcgt cggagaggct
       61 tgcttcctgc gcgatctttc cgaaagtgga gcgaatcgct tcccgcgtgg ctgcttctcc
      121 acgaagtgtt acggcgcggg cgttcacgtt gcccgttttc accagcttgt ccaactcacg
      181 cgcccagtcg ttgaatcttt gctcgtaatc gggttcgcct cccaatccgg caaccgtgac
      241 gtagaacgta gccgccggca aagaaaacga acaggcaacg agcagcagga cccacttcat
      301 acggcacccc accgccgccg caacagccac tcggaggcgc gcaacagcaa ggccagcaga
      361 aacacagccg gcatgttcca gagatcgcga gtctcgcgcg tagcgatacc ggcctccgaa
      421 tattggatct cgtcggcaat attcgacagg ttgcgcgggt tgtaatagcg gccgccagtc
      481 tcggatgcga gcatttccag cagttcgcga ttctgctcgg catgaaactt ctcggccact
      541 ccatcctcgc ggcggaacat cagcacgtcg cggccgatct cctcgttccc tcgtttcgcc
      601 agcatttcgg cgagatacga accgggtcgt tccgccaccc attccgccgt gtagtccccg
      661 ttctgaagag gatccggcga caacgttacc accgccgaag tgttctccgg cccgatgatg
      721 cgggcttcca cggtcgcatc cgccatcgcg agatagctgg tgtcgtggac ccgtgcgcga
      781 agctgaagac ggccgttgtc gtacaacatc ggttgaggac tggacgacaa aacacggccc
      841 ggcgtttcag tggccagcca gcgcagcaac tgttgccaga acatctcgtg ggtgcggtcc
      901 gcgagcggtt gctgcatctg ccatcgccaa ctgccggcgg aggcgaacac ggcggtccgg
      961 cccatgccgt acttctgcgt taccagcagc gggaaccggc ctttgctggt gggcaggaac
     1021 tctgccagaa ccactgcgcc cgctttcggc tcgccgactt cctgaaaatt ggcgagatag
     1081 ggaagcttct tccaacgatc ggcattggag gcgggatcgc cttccaggcg gcaaatgagg
     1141 ctgtcgcggc cggctggcgt aagctcgaca gtggccggat cgcgatggaa cgtgttcttc
     1201 gcattcggca gcgacaccgg cagcaggtcc gccagcggag aagtgccgta tccgccatcg
     1261 gccaatccgg accggccggc cagaaaccgt aacccgccgc cgcgccggtc gacaaattgc
     1321 cggatcagct cctgttgcgt ggaggtgaag gaggtgacct cgataccgcc gaggatgatc
     1381 gcctggtacc cgaagaggtc ctccaccctg gaaggaaagc cgtcctccaa ttccttcgga
     1441 ttgtcgatgc cctggcggta aatcttgttc tgcgtggtcc gcaggatgga aaccagactg
     1501 aggccccggt cctcctccat cgcgcgccgg atgaatttga actcccacct gggctcgcct
     1561 tcgatataga gaatgcgagg ccggtcggac tggacgttca ccagccgggt gagagaattg
     1621 ttcgcaccgt tctcctcgtt ggcgagagcg tccacaacga actggaggtt tttcgccccg
     1681 gcgatgcctg cattgaacac gatcgcctcg gtttgctgaa ccccatccgg ctttaaggta
     1741 acctgctggg aagcaagagt cttcccgttt tcgacaactt tcagcgtggc ttttcctccg
     1801 gcatagccac gctgccggaa actgacatag gcgctgagcc gcgaatcggc caaagtacgc
     1861 ggagccacct gcacgtcgga gatttcgaga tcgcgcgcga attgctcgcg cccgataccc
     1921 acggtgtgga tcggaaggcg gcggcttcgc acattcttga tcatctccag atcgatacca
     1981 gcggtattgt cggctccatc gctcaatagc agaactgcac caatgggaag gctcgccgat
     2041 tcggcaacca cctgtttgag cgcatcgttg atccgcgtgg aaggcatgga tgccttgagg
     2101 tcctggagct tatcgatgcg ctccagatgt gagccgagac gatagaggcg aacctgaaaa
     2161 cgctcgccaa gcttttccag aatcccgctt cccagcgcgg actgcgcaat gctcaacctt
     2221 gtcccgccct cttcgggaat cgccatgctt tgggagtcgt cgaccacaac ggcaatgacg
     2281 ttctgctggg gttttagcgt tgcgatactg atggccggct gccacagcag cagaaacacc
     2341 agcgcggcca gagcggtttg aagtccccat atagccgcct tgcggcttcc ccgaatcggg
     2401 ctccgttcgc cggccgggcg gcgccacact gctatcgcaa gcaggacggc agccacggcg
     2461 atcagcgccg gaagtatcca cacaggccac gcactcagga gtaccagtgt gcccttagag
     2521 aaaaccgtgg ctggatactt aaacaaaaat tcaaacatct ttggctacgg aacccgcctc
     2581 aatgagtcat tgagtaaatg atgtagttga taccgatccg gtatgcaagc gacgcatact
     2641 tttccggata caaggggtga tcggcccatt cccatgcatc ccccaagtcc atgttgtggc
     2701 aaattgccac catcagccgc cctctatcgt cgtagatccc cctccagcga gcctcaaggc
     2761 cgtcgtattc ataaatgcgt cctgtttgaa acatgacgat tccgggcacc tggaatcttt
     2821 cgtccaggtc gtataagaca tgaaagatag gatcgctgtt ctcgagatcg accaccgggc
     2881 gatcgggaaa cacccggctc atgctggcca tgaagacatc ccactcaaag gtgccgtgga
     2941 aatcgtcggt catcaggaat ccgcctcgca gcagatattc ccgtagtttt gcacattgcg
     3001 cgtcggtcag gtcccaatgt cccggttcca cgacatacac ccagggcgag tcgaaaatct
     3061 catcgctatc gaggtctact accttttcga cggatcgtgc atgaattcgg gtcagacggc
     3121 gcacaccttg cacaaactgg cgatccgctt tgggataatc gatggcccag ttcccacgca
     3181 tcgcccaata gccgcccata ccgctgcccc ggaacgaagg ataccgcagg cgcgcgaaaa
     3241 cccactcggt cttctcgttg gcatcgggag gagtaataat ggggtcgtcc tcgcggtcaa
     3301 ggaattgttg aagaacgctc cgctggagtg cgaacgcggc gcccagtgcc gcaaaaaaaa
     3361 tcgcgattgc aatgaaactg ccacggggac gcattcaaac ccctattttt cttaggataa
     3421 cacagcgtgc gagaccggct gtttgacgtt agaatgggcc gcagtgtatt gctgcgacga
     3481 acttcaacaa ctcgccggcc gtgaactggt acgcatcggt ccggtgcaca ctcttaccaa
     3541 cggacgcatt ctgacggaga tcgatacgga atattttctg gttttcgggg aggaacggcc
     3601 cacgtatgcg ggcatcaact attgcccctt ctgtggacgg gctctctcgc gcgggctctg
     3661 gaacctggag aagaagaagc aggggcggta aaaaagggcc ccgggattgt ccccggagcc
     3721 cacggaagca ctacgggcgg agatcctgct cctgaatgaa ggtggtccac tcttctgtcc
     3781 agtcataatc tccgaaggcg ccaatgtagt tcgccgtctg atcgaagaag ccgtcatcgg
     3841 gcggctgaac ccaggtggcg cgataaaccg gggagccgag catgggacgg aaatccggat
     3901 cgctgcgttc gagcggtctc cgcaacatcg ggtctgcgac gacgaagttc cggccctgct
     3961 gtccgccgct ggcaaaaggc agaaaaacat cctgaacctg cgcatcgagc gagttcgcac
     4021 gccccgactc cttgccgtta tcccacatca gtattccatc ggcagtgaaa tcgccgctgt
     4081 cgatatgcgg ctggatgcta ctgaaatcgg atccgccgaa agccctggtg gtccagttgt
     4141 agcagatcac gttgttcgtg ctgccgcccg cgcccctgcg gaaatacagg catggcgagt
     4201 cggattcgtc gaatccccgg gccgcgcttc ccacgaatgt cgcgttccaa aggttcagct
     4261 ttcctaacgg ccgagcagcg aagtcacgtt cgtaattatc ggtctccact gccctgttcg
     4321 aaaggtcttc gttggcgacg gcgactacgt gctgaacacg gccgttgtag ccgatttgta
     4381 catccacgta atcgtcggcc ccgtaagtgc caacaagata tttggcgtcg ttatttccgc
     4441 cgaaccactc gaaggaatcg tccaggccgt aatgcgcctg caggtattcg ctggtggtgg
     4501 ctttgccgca accaccccag gtgaacgagt tcgtttcttc gttcggccgc agcagtgctc
     4561 cggcgaactc cactcgtaca tatcgcagcg tgccgcagtt gtggtccgca ttcgaaccgc
     4621 cgtagcgggt ttcgggcatc tcgggaagac cttcgatggt caactgcccg ctcggatcgt
     4681 tgagcggagc cgtgcccagc aggagaagcc caccccagtc gccccgctgc cgctgcccga
     4741 gcggctgtga cgatgtcatg ataatcggcc gcgagcgcgt tccgttcgct atcaggcgcc
     4801 cctgtgtcgt gaccagcagg acggacggcg gctgggaacc aggcatgccg ataatgaacg
     4861 tcccaggttc gatggtcagc accgcgttat tccgaaccgc aacgatgcct tgcactcgat
     4921 acgccctatc gttggtaaga gtgcgatttt cattgatgga gcttggcagg tcctcgatcg
     4981 cgctgacaac gccgaatttg aagtaagcgg cctttacgac gcgcgtgccc gtgtaatccc
     5041 gaagctgcac cacgatggta tgcataccca gctcgcccgg cgcggtgagc gctgtcgatg
     5101 ctacgcttcc accctctccg gcgagtacac ctttctccgc cgccggcaaa cgtacaatac
     5161 ggaacccttg ctccacggtg ttcccgaaga tatcggtagg ggttgtatcg ttgccaggca
     5221 aatagcttct ggtgcctgtc tgattgttct ggtggaatgc cactaaggta tatggataca
     5281 aatcgtcatt aggcttgatc gtccagcgaa cggtgatcgg ttctcccggc tggtacacgt
     5341 atttgtcggt ccaaatccac atcgcggcgc gcgtgtcgac ctgtgaataa gaatcgatct
     5401 gcctccttgc gtcctgaaga tcgacggttc ggaatgccag aggggctccc gctcctgctg
     5461 ctaccaatgg ccacactgca agcgctgctg tcgcgaagct ggtaattcgc atagctggtg
     5521 tttatccttt cgttgatgtt tgagaattcc ggttagaaga atgagtaact ggtgcccacc
     5581 gtgaaagtcc ttccgatgcg gaacgcccgc accagaatgt cggcttgggt gaaacggtaa
     5641 tgattgtctg taaggttctc ggcgccgaaa cggagaatcc acttaccggt ttcgtccagg
     5701 ttgacgtgat agacgaaatc aagcatggtc gttcgttcct gatatacgtc gggtaactgg
     5761 aaggtcccca cgtctgtgat ccggcgcgat accgaattga catagaacct tgcgttggac
     5821 cgccatgacg gcttcaccca ttccgtgatg acgttgtaaa tcaaccgcga ttggccaacc
     5881 agcggccgcg ttcgggaagt aagttgcggg aagcggtccg tcgggatccc cacgttggaa
     5941 tccacgaagg tgagattggt ttgaatcgca aaaggagaaa gtctgccgga gaaggatccg
     6001 aggttcttcc tgaactcgaa ttccacgcct tgatttctcg cttcgggcac gttcaggaag
     6061 ctttgacgca actccgacgc cgtcggcctg tagatctgct cgatcggatc ggtgaagctc
     6121 ttgtagaaat aaccggcggc gatcacctgg ttgccgccga gaaaccactc ccaccgcgcg
     6181 tcgaagttgt caatggtagc ccgttgtaat tccggatttc caaccgtcga atagccgccc
     6241 acaacgttcg tgaattcgaa cggggaaagt tcccggaagt cgggccggtt caccgtgcgc
     6301 ccgtaggcga accggaggtt ctgcctgccg ttcagagcat agatgacatt cacggccggc
     6361 aggaaatcgc gattaaccag gttggcgacc gagggcatcg agcccggaac cagcggatcg
     6421 atggtgctga cgacaacatc ggcatcttca atgcgcgcgc cgccgatgaa ccgccaccgc
     6481 cggcctatcg ccagatcgat catcccgtac ccgccataca cgtccatgct ggctgtatag
     6541 gtgtctgtgc ctcgcgtgat ctcccggacg acgaaaccat ccggccggat attcgcttcg
     6601 cccagaacct cgtttgccgg cttggtgaag tcgatggtct gagcccgcac cgggaaaaag
     6661 cggaagcgcc gtccttcaaa atcgcgccgc cgcgtcgtgg cgcggaaccc cactttaaag
     6721 agtccactga tcgatccctt gaagaacggt ttcgaccagt cgccttgcgg ctcgtagatg
     6781 cgatcgttca atccattgaa gaagcgcatg ccggattcgg gcaggttcag atattcgtat
     6841 ctggcgccgt ccgccgcccg gccgtatatg gtctcccgaa ggtccggttc attgcgagtc
     6901 gagtaagagt aggtgaactg ccagtgaaag acgctgtttc ccaagcgcgc aacggcatgg
     6961 tctccttcga tggagctgga cagcaggcct ctctccaccc atctgagccg cgtgtcctga
     7021 atatagttat tgttgccgcc gttgagacct tcgagaaagc gcgcctcttt gtcggtatcc
     7081 cgtgtaacgg tgttccgcca gatcaacttc gcaccggggc tgagccggta ggcggcgttg
     7141 aacactcccc cgatgcgaac cgattctgtg tcggtgttga attccggata atcgctgaag
     7201 atctgcggcc ggcctccgcc ggaattcacg aggaacctgc gaagctccgg ataccgctgc
     7261 ggcgcattac tgaaggtgag cgctccgaca agcccgagcc gtccccagct gttgcctccc
     7321 gccaccgagt acgtctggct gggccgcatg gaatctaccg gctcgggttc gtagttgttg
     7381 gagaatgcac gtccgaactg ctgaaactgt tgctcgttga agtttccgac aaacagacgt
     7441 tggtctggag gtatgctccc cggcaggctg cgggaaccgt cgtcaaaacc ggtccaatca
     7501 cgggaaccgc ctcggtatgt gttgaagcgg ttgaaggttg agagcgtgtt gaaaccgtag
     7561 ttgacggaaa cgctcagcat cttctcgacc ggaaactcta cagtgcgaag ctgtaccagt
     7621 cctc
//
LOCUS       DASTMK010000517         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_315460, whole genome shotgun sequence.
ACCESSION   DASTMK010000517 DASTMK010000000
VERSION     DASTMK010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315460"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFQ79_21470"
     CDS             <1..1002
                     /locus_tag="VFQ79_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142305.1"
                     /translation="NQVSLAGAGFVLRYLEHIAVRLGPLVAVLGLFVSAGSVKGIFRN
                     PRTTVKVALTLVPPALALAYYTTITHEVGGSHRFSYPTYFYLVIAAAAVATLAFKRLP
                     DLNRTRLAALAAAMVAILFATDGNWRSLLRGGAARPPDEFSEYHFQIARVLEATRLGP
                     RMTILCDAAGIIPFVTEVNLLDRVGLNDNFLSGRHPISPEEREQYIWSRNPDVYIGFE
                     PPASSSAARAEEDPRMKTGYLNHLMQFHRTGGVVIIENRTFLMRPELWHTRMRELRDN
                     WIWLGEINWPGWRISGLKSFVYVRRNSSHFDTLAPALKRLVAVEPERVDLNRTPSSL"
     gene            1011..2909
                     /locus_tag="VFQ79_21475"
     CDS             1011..2909
                     /locus_tag="VFQ79_21475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEU0142306.1"
                     /translation="MELRELLIRKDPLFWPQLREQSGQASNFESLISLCTLRKRAEAA
                     GLAPAEPRETLRIAFLGGCSLYPLNELARHMLSVEGYACEVFTGDFDNYASEIMDEAS
                     ALYRFAPQVVFLLPSPGRCKYQGGWTDARETQQAQAVETGSQLLGLCRKVHERTGAEV
                     LLSNFMLPGGFDPGAYRTRTLASDWSFRKVVNLELGLNAPPYVHLCDLEFLAYRRGAS
                     ASEDARGWFETKQPCAPDLLVDIAKEIAVLVRGFRKGHKKVLVLDLDNTLWGGVVGDD
                     GLEGIEIGDTSPRGEAFKAFQRYILSLAGRGILLAVSSKNDHEKAIEPFDKHPEMVLR
                     PQHFASFKANWNPKSENIREMAAELNLGLDSFVFVDDNPAEIEIVRQFLPEVSTILLG
                     PDPAAYVRELEDSRYFEPRSITAEDAGRTAQYRQEAERRTLLTSATDMDAYLASLEME
                     ATLREFNPIDAPRIAQLINKSNQFNLTTRRRTEAEVLELARDPGAAAFTIRLRDRFGD
                     HGLIAVVIGKIVPGEAGTEFEIDTWLMSCRVLKRQVEQETLNEIVRLARLHSCAEIRG
                     VYIPTAKNGMVRDLYPQMGFSLASGGETRTEYLLAARSYEAVPTQIRIAGRSYESRSS
                     HLASTASF"
     gene            2866..3111
                     /locus_tag="VFQ79_21480"
     CDS             2866..3111
                     /locus_tag="VFQ79_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0142307.1"
                     /translation="MNQDQVISRLQQVFDNIFLEPVKLTPQLSAQDVEEWDSLTQISL
                     MVAVEKEFNVRFRVGEVEATKNVGEFADLIARRVNGN"
     gene            3126..3260
                     /locus_tag="VFQ79_21485"
     CDS             3126..3260
                     /locus_tag="VFQ79_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142308.1"
                     /translation="MLRNTLSPGNVITIVTVVLSIAAIALFLFLPSDSLDIGIVYGGF
                     "
     gene            3260..4642
                     /locus_tag="VFQ79_21490"
     CDS             3260..4642
                     /locus_tag="VFQ79_21490"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEU0142309.1"
                     /translation="MAAPAVTRPVLTPGLPRTGEWLKTGLLAALGVAGLAIFLVWDPP
                     DYRPLGTVQAARFLLGALTYLAAARAVLYLKSEGLRAMLFAVINVAAIGLFFDMSDGR
                     RRFLFLLAAYLSMVSFHYVMMRLFARRAGWWPWMAFAAPIAALVVLRYAAALFDPVFA
                     LVPGILTGPLAAFFIGLSYMAFRLSHLVLEVRNGVVARPGFWQYLGFAFFLPTLVVGP
                     INPYSNHAESLGAPDRTATPIGRCLLRILMGAAKYQFLGNIFNQLSYAGLLLDGYPHS
                     PVDLFVAAVAYHLYLYCNFSGFCDMAIGGAGLLGIQVKENFRNPLGARNVQDYWTRWH
                     ITLSTYTRDVLFSPLSKFLVVRLGPKRANHAIAAAIVFVFLLVGIWHGTGANFAVYGA
                     LHGLGVASVHYYNVWLKKRLGKQRYKAYHESRLVHAVAVAMTFLFVTASLFFFANDFE
                     TARQILHAIQ"
     gene            4629..5063
                     /locus_tag="VFQ79_21495"
     CDS             4629..5063
                     /locus_tag="VFQ79_21495"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEU0142310.1"
                     /translation="MPSNNSYSFEDLHEGMMVEREYTIDATVYETFLSIFPDKSPVHV
                     EDGYAKANGFDGIVMHGAILNGFLSHFVGMCFPGRRSLELSVDMRYLKPCYMGDKVVL
                     RGTISQKLESRRVVVLDLAYENRSRSYTAARGRVQVAIMGEE"
BASE COUNT          964 a         1541 c         1537 g         1104 t
ORIGIN      
        1 aaccaggttt cgctggccgg cgccggtttc gtgctgcggt atctggagca cattgccgtc
       61 cgcctcggcc cgctggttgc cgtgctggga ttgttcgtct ccgccggatc cgtcaaagga
      121 attttccgca acccacgcac caccgtcaaa gtggcactga ccctggttcc gcctgcgctc
      181 gcgctggcct actacacgac catcacgcat gaagtgggtg gaagtcacag gttctcctat
      241 cccacatatt tttacctggt cattgccgcg gcggccgtgg ccactctggc cttcaaacga
      301 ctgccggacc tgaacagaac ccgcctggcg gccctcgcgg cggcgatggt ggccatcctt
      361 ttcgcaaccg atggcaactg gcgctcactg ctccggggtg gtgcggcgcg gccgccggat
      421 gaattctccg aataccactt ccagattgcc cgggtgctcg aagccacacg cctcggaccc
      481 cgcatgacca tcctttgcga tgctgccgga atcattccgt ttgtgacgga agtgaacctg
      541 ttagaccggg taggcctcaa tgacaacttt ctttcgggac ggcatccgat cagcccggaa
      601 gagcgggagc aatacatctg gagccgcaac cccgacgtgt acatcggctt tgaaccgcca
      661 gcttcctcca gcgccgcccg cgcggaagag gatccccgca tgaaaacggg gtacctgaac
      721 cacctgatgc agtttcatcg cacaggcggc gtggtgatca tcgagaaccg tacgtttctc
      781 atgcggccgg agctatggca cacgcgcatg cgcgagctga gggacaactg gatctggctg
      841 ggcgaaatca attggccggg atggcgcatc tccggcctca aaagcttcgt atatgtgcgc
      901 aggaactctt cgcacttcga caccctcgct cccgcgttga agcggcttgt agcggtggag
      961 ccggagcggg tcgatttgaa ccgcacacca tcttccttat aggtgtagcc atggaactca
     1021 gggaattact gatccgtaaa gacccgctgt tctggccgca gttgcgcgaa cagtccgggc
     1081 aggcttcgaa tttcgaaagc ctcatttcgc tttgtacgtt acgcaagaga gccgaagcgg
     1141 cgggactcgc gccggccgaa ccccgggaga cgctgcgaat cgcttttctc ggcgggtgca
     1201 gcctctatcc gctcaatgag ctcgcccggc atatgctctc cgtcgaaggt tatgcctgcg
     1261 aggtctttac cggcgacttc gacaactatg cctccgaaat catggacgaa gcaagcgcgc
     1321 tttaccgctt cgctccgcag gtggtatttc ttctgccctc acccggacgt tgtaaatatc
     1381 agggggggtg gacggatgcc cgtgaaacgc aacaggcgca agcggtagag accggctcgc
     1441 agctactggg gttgtgccgc aaggtgcatg agcgcaccgg ggcggaagtt ctcctttcca
     1501 atttcatgct gccgggcggg ttcgatcccg gcgcttaccg cacgcgcacg ctcgcatccg
     1561 attggagctt ccgcaaggtg gtgaacctgg agctgggcct caacgctccg ccttatgttc
     1621 atctctgcga tctcgagttt ctcgcctatc gccgcggcgc gtccgcatcc gaagatgccc
     1681 gcgggtggtt tgaaaccaaa cagccttgcg cacccgacct gcttgtcgac atcgccaaag
     1741 aaattgccgt gctggtgcga ggttttcgaa aagggcacaa gaaagtgctg gtactcgatc
     1801 tggacaatac actgtggggc ggcgttgtgg gcgacgacgg cctggaggga atcgagatcg
     1861 gggacacctc gccgcgcggg gaagcgttca aggccttcca gcgctatatc ctctcgctgg
     1921 cgggccgggg catcctcctc gccgtatcga gcaagaacga tcacgagaaa gccatcgagc
     1981 cgttcgacaa gcacccggag atggtgctac gccctcaaca cttcgcttcg ttcaaagcca
     2041 actggaatcc caagtcggag aacatccgcg agatggccgc cgagctgaac ctcggcctgg
     2101 atagctttgt attcgtggac gacaacccgg cggagatcga aattgtccgg cagtttctgc
     2161 ccgaggtctc caccatcctg ctggggcccg atcccgccgc ttatgtccgc gaactcgagg
     2221 actcgcgcta cttcgagccg cgcagcatta cggccgaaga tgccggacgg acggcgcagt
     2281 acaggcagga ggcggagcgc aggacgctgc ttacttccgc caccgacatg gacgcctacc
     2341 tggcgtcgct cgagatggaa gccacccttc gcgagttcaa tccgatcgat gcgccgcgca
     2401 tcgcgcagct tatcaacaag agcaaccaat tcaatctcac cacccgccgc cggacggagg
     2461 ccgaagtgct ggagctggca cgggacccgg gcgcggcggc cttcaccatc cgcctgcggg
     2521 accgcttcgg cgatcacggt ctcattgccg tcgtcatcgg aaagatcgtc ccgggcgaag
     2581 cgggaacgga gtttgaaatc gacacctggc tgatgagttg ccgtgtattg aaacggcagg
     2641 tggagcagga gacgttgaac gaaatcgtgc ggctggcgcg cctccattcc tgcgcggaaa
     2701 tacggggcgt gtatatcccc accgcaaaga atggaatggt gcgggacctg tatccgcaga
     2761 tgggctttag ccttgcgtcc ggcggcgaaa cccgtacgga atatctgttg gccgcccgct
     2821 cgtatgaagc tgtccctacc caaatccgca ttgccggaag gagttatgaa tcaagatcaa
     2881 gtcatctcgc gtctacagca agtttttgac aacatctttc tcgaacctgt aaagctgacg
     2941 cctcaactga gcgcgcagga cgtagaggag tgggactcgc tgacccagat ctcgctgatg
     3001 gtggcggtgg agaaggaatt taacgtgcgg ttccgcgtgg gggaagtcga agctacgaaa
     3061 aacgtcggcg agtttgcgga cctgatcgcg cggcgtgtaa acggcaactg atccctcttt
     3121 ttgaaatgct caggaatacg ctctccccgg ggaacgtcat cacgatcgtc acggtcgtgc
     3181 tcagtattgc ggcgattgcc cttttcctgt tcctgccaag cgattcgctc gacataggca
     3241 tcgtgtacgg aggcttttga tggcagcgcc cgcggtgacg agaccggttc ttacgcccgg
     3301 gttgccccgg accggcgagt ggctgaagac cggtctgctg gctgctctcg gtgtggccgg
     3361 gctggcgata tttctggtct gggatcctcc ggactaccgg cctctcggaa ccgttcaggc
     3421 agcccggttc ctgctcggcg cgctgaccta cctggctgcg gcaagagccg tactctatct
     3481 gaaatcggaa ggccttcggg ccatgctgtt cgcggtaatc aatgtcgccg ctatcggcct
     3541 gttcttcgac atgagcgacg gccggcggcg gttccttttt ctcctggcgg cgtatctttc
     3601 catggtttcg tttcactacg tgatgatgcg cctgtttgcc cggcgagccg gctggtggcc
     3661 gtggatggca ttcgccgcgc ccattgccgc cctggttgtg ctgcgctatg ccgccgctct
     3721 gttcgatccg gtgtttgcac tggttccggg catcctcacg gggccgctgg cagctttctt
     3781 catcgggctt tcctacatgg cgttccggct cagccacctg gtgctcgaag tgagaaacgg
     3841 cgtggtcgcc agacccggat tctggcaata tctggggttt gcgttcttcc tgcccacgct
     3901 cgtagtcggg cccattaatc cttacagcaa ccacgcggaa tcgctgggag cacccgatcg
     3961 cacggcgaca cccatcggcc gctgtttgct gcgcatcctg atgggcgcgg cgaaatacca
     4021 atttttgggg aatattttca accaactttc ctatgcgggc ctgctgctcg acggttaccc
     4081 gcattcgccg gtagatcttt tcgtcgccgc cgtcgcctac catctgtatc tgtactgcaa
     4141 cttttcaggg ttctgcgaca tggcgatcgg cggcgcgggc ctcctgggta tccaggtgaa
     4201 agagaacttc cgtaatcccc tgggggcgcg gaacgtgcag gattactgga cacggtggca
     4261 cattacgctc tccacgtaca cgcgggatgt actgttttcg ccgctgtcga agtttctggt
     4321 cgtccggctg ggaccgaaga gggccaatca cgcgattgcc gccgccatcg tgtttgtgtt
     4381 tctgctggta ggaatctggc atggaaccgg agcgaatttt gccgtgtatg gagctcttca
     4441 cggcctgggc gtcgcatccg ttcattacta caacgtgtgg ctcaagaaac gcctgggcaa
     4501 gcagcgctac aaggcctatc acgaaagccg cctcgtccat gccgtggccg tcgccatgac
     4561 gttcctgttt gtgacggcca gcctgttctt cttcgcgaac gatttcgaaa cggcccggca
     4621 gattctgcat gccatccaat aacagctact ctttcgaaga cctgcacgag ggaatgatgg
     4681 tcgagcgtga atacacgatc gacgcgaccg tgtatgaaac tttcctgtcc atctttcccg
     4741 acaagagccc cgtgcacgtc gaggacggct atgcgaaagc caacggcttt gacggcattg
     4801 tcatgcacgg cgctatcctg aacgggttcc tgtcgcattt cgtgggcatg tgttttccgg
     4861 gccgccgttc gctcgaattg tcggtggata tgcgttacct gaaaccttgt tacatgggag
     4921 acaaggtagt tcttcgcggt accatatccc agaaactgga gagccgccgc gtagtagttc
     4981 tcgatcttgc ttacgaaaac cggagccgga gttacactgc cgccagggga cgcgtgcagg
     5041 ttgccatcat gggggaagaa taaggggggc cgttgatgcc gcgaagtact gttgccgctt
     5101 tatatacatc gccggagagg gtgctcgacg atattggacg cctggc
//
LOCUS       DASTMK010000518        10822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_59821, whole genome shotgun sequence.
ACCESSION   DASTMK010000518 DASTMK010000000
VERSION     DASTMK010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59821"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFQ79_21500"
     CDS             <1..991
                     /locus_tag="VFQ79_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0142311.1"
                     /translation="FLGANLEGYGCAGMSNVEYGLIMQELERGDSGLRSFVSVQGALV
                     MYPILTYGSEQQKQTWLPKLQAGEAIGCFGLTEPGFGSNPGGMTTKATRAGDCWVLNG
                     EKTWITNGSVAHIAVVWARTDEGIRGFLVEKGTAGYSTADIHGKLSMRASVTSSLSFQ
                     DCRIPSDNMLPGAKGLKAALSCLTQARYGIGWGVIGAAMDCYETARRYSLARKQFDGR
                     PIASHQLVQEKLAWMITEITKAQLLALHVGRLKDGGKAEAAHISMLKRNNVAIALDCA
                     RLSRDLLGANGIMDEYPLMRHLCNLETVKTYEGTDHIHALVIGERVTGVAAYK"
     gene            1002..1691
                     /locus_tag="VFQ79_21505"
     CDS             1002..1691
                     /locus_tag="VFQ79_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0142312.1"
                     /translation="MSILRLEQVSKNYQTNGTTVSALRDITLAVKPGEFVALLGPSGC
                     GKSTLLNLAGAMDFPTSGQVILRGIATSALDDSALTRLRRETIGFVFQSFQLLPTLTV
                     AENVELPLLLAGAKGARAAALRVLEQVEMDGFASRMPYQLSGGQMQRVAIARALIHTP
                     AILLADEPTGNLDSATGDLILALLRRIANTGQSTVMLATHSAEAATLADTVVRMRDGR
                     IQEIRENARKP"
     gene            1698..4211
                     /locus_tag="VFQ79_21510"
     CDS             1698..4211
                     /locus_tag="VFQ79_21510"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142313.1"
                     /translation="MPYWKLFYRLIVRPLRREPVRTLLTAFAVALGVAVVLGIELAGT
                     AAAGSFRSSLEALADDADLEITATGGLPAEVLAQVAGLPHSFRFEPRMEDFAFLPARN
                     RTVPWIGVDFIANAPEQGASAGDGDPADRFRKEQVWVSANLGYKTGDRIPVLIGDTLS
                     EFHVGGVLSKQPGEVLVADLALTDRLFRREGKLDRILVHTPSGRSVAEWEQLLRSALP
                     PGAGVGPQGSSTDENRRMLAAFRWNLRVLSYIALIVGAFLIYNTISVSVVRRRAEIGI
                     VRALGATRAAVLLGFVGEAVCFGLSGALAGIALGRFLAESAVGLIASTVQSLYVSSTP
                     APVELSWKIALLGIGMGITVAVLSALSPAWEAARAAPLEAMARGRGEHRVQIRKLRGL
                     LASGLLSAGAWIASMQDAMDGKSLFGYLAALLLIAASAFAIPALVSVLAASTAAWMRC
                     LFGVEALLAARGLAGSLRRTSVLVGALSTAIAMMAAVGIMVGSFRQTVVLWMEDRLQA
                     DLYLRPAAPGGADRHPVLSPDLADRIAALPEVAAVDRFRAYSIRYGGMPATLGAGDAR
                     IAGRYGRRAFLSGAKPQEVFEQLTGTDSVIVSEPFANKHGVKAGDILSLPITGQPVRL
                     RVLDVYYDYSSERGYILMDRDRLLQYLPDPAPSNIAVYLRPDVPLEQGRDAIEPVLKG
                     RRVVVLSNRSLREEALWVFDRTFAITYALEAVAVLVAVMGVAGALLALVIDRRREFGL
                     LRFLGGAQSQVRRIILFEAGLLGLFANAAGLLLGFFLSLLLIFVINKQSFGWTIQFHW
                     PVGVLMGALSLVYLATIAAALYPARVAAQLNPIEVIHEE"
     gene            4201..5307
                     /locus_tag="VFQ79_21515"
     CDS             4201..5307
                     /locus_tag="VFQ79_21515"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEU0142314.1"
                     /translation="MRSSRVLALLLLAAMASLGADSDWRVALPGYAYSFPRDHFSHPE
                     FRTEWWYYTGNLQTQNGRRFGFELTFFRHAVDSTKPESVWDVRDVWLAHLVLSDIDGG
                     RFLHVERINRSGPGIAGADLKTARVWNGNWQVHWMLDAARPAAFTRQTLDAVSEQFRI
                     HLSLESEKPPVIHGRDGVSQKAEGRGRASHYISLTRLKANGSIAIGGEEFKVEGLAWM
                     DHEFFTHQLAPNQTGWDWLSLQFEDGTELMLFRLRRKDGSIDPYSAGTFVDAGGKTKH
                     LDLSGFSLVPEGNVWRSPKTGARYPVAWSISVPELRLAARVRTRLPQQELTSRHSGVG
                     AYWEGAIEITGTRDGRPLNGRGYLEMTGYAAPVQ"
     gene            complement(5313..5903)
                     /locus_tag="VFQ79_21520"
     CDS             complement(5313..5903)
                     /locus_tag="VFQ79_21520"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0142315.1"
                     /translation="MEAGEPYFYLPYAQTNFSSMYLIATTGLDPLQLSRPLRAEVAAI
                     DPSVPVVEVTTMKLLVRSQLYQQQVFATIVGGLGAIGLLLAAAGLYGLISYSVTLRTR
                     EIGIRMALGAQRRCALGMVLRQTLVLALIGTGAGLIGAVFTVRILSSLVYGVSVRDPI
                     TFGAVIVSMLAVAMLAGYIPARRSTQINPMAALRHE"
     gene            complement(5923..7545)
                     /locus_tag="VFQ79_21525"
     CDS             complement(5923..7545)
                     /locus_tag="VFQ79_21525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142316.1"
                     /translation="MSTRRKSNAARPAAFESWKISRATFAMRPGCLPGAGGSPTPRSA
                     RDALSYAEYLDYQSQAKSFSGLLATQRRGPILTGDGFAESTMSNVVSENYFNVLGVEA
                     QLGRVFTAADANTGQRVVVMSHNLWQRRFGGDSGIIGRSVRLNGPYVVVGVAPKEFRG
                     TELWQDSDFWIPITSWDPSERNLRESRNWEVLGRLRPGVSIEAAQAEIAGIAANLERA
                     YPKFNKGCRAVLATATEHLFQSTKYVPFILLGIVAVVLLIACANLANLLLARAQGRSR
                     EIGVRLAMGASRGRLVSQLMAESALLCALGTAAGLLLAAWLVSLLPAVIIPPANSYLH
                     MDFRLDQRVLAFTLITSLVTVFIFGLAPALRASRIDLTLVIKGGDLPDTRRGRLPARS
                     ALVVVQMALSLMLLVGAGLLVRTFIYGMNLDLGFERRDVLVAEISPPYDGPRNRAFYR
                     QLIERLKAIPGIRESTLALRAPLSGSGGGLAAQVTIPGRSIQPGDPLSSIKYPAVDLN
                     YFRTLGIRLLRGRDFDAQDGPGLRVYDRGDCP"
     gene            complement(7730..8062)
                     /locus_tag="VFQ79_21530"
     CDS             complement(7730..8062)
                     /locus_tag="VFQ79_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0142317.1"
                     /translation="MADDRLDLPQGTLDLLILKTLALSPQHGWAITERLQQISSDALR
                     VGQGSLYPALHRLERRGWIRAKWGVSENNRKAKYYELTNAGRKQLKAETEAWRKLAAV
                     IGQVLDFA"
     gene            8239..>10822
                     /locus_tag="VFQ79_21535"
     CDS             8239..>10822
                     /locus_tag="VFQ79_21535"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142318.1"
                     /translation="MRKLLILTFPLLVSVHAYGQEVRANITGLVTDPTGAPVAEATVI
                     VTNVARNTSVQTATNETGNYFSPFLVPGTYRVRVERAGFKSFIRDNIVLEAQDRARVD
                     VQLEVGELAQSVTVSADVSLLQTETASRGQVISTQLIENVPTQGRNPFQLAWSAPGVI
                     KTGDWRYLRPFDIGGTSGFSINGGVRGQNEVLLDGISNVRGSGTVIHVPTMEAVQEFK
                     VLTNTYDAQYGRTAGGIVTIVTKSGGNAFHGTLFEYFQVEELNANQFELNRAGTPKPP
                     MNINTFGFQLSGPVYVPKVFDGRNRLFWLLSYEGLRQRSADPGAANFPLAEWRNGDFS
                     TLLNAQGQQVRIYDPLTTNASGQREQFPGNRIPASRIDPIARAVMNLYPMPNSPGDGP
                     AHVNNYIYPSRWVADMDEWIGRLDLMVNPSNVVFFRYGQNPFSEYRSIVWGTNEAEPT
                     GNAPLIRNGRNWTIDWTSTLSPHMTFNLRAGLNRWEEQTGNTYGANYDPRQLGFASSL
                     VSQFTKLQFPRFDLDTYQAIGSDRLLALGANDTYSIQPNLSLVVGTHFLKFGGEGRRY
                     NDNTNNPGLASGLYTFRKNWTQERALQGDAVSGNELATFLLGYPTTAYVDRNIDPAFT
                     NNYFAAFVQDDWKITPRLSLNLGLRWDYETPMVERFDRMVRGLDFDAASPLASQASGM
                     NLKGALLFAGVGGQPRGSFEPDRNNFQPRVGAALRLGQRWVVRGGYGLYYLGQNQRGS
                     NQGFSQRTNAIVSTDGNLTPAVTLANAFANLPGGQLLSAVGAGNGAASFLGESLTVNY
                     LNRPLPYAHQYSVDIERELPGNLLVDVAYVGNITRRLPVNNVNLNAIPAEELGRRTPA
                     GAIDT"
BASE COUNT         2121 a         3251 c         3359 g         2091 t
ORIGIN      
        1 ttttctgggc gccaacctgg aaggctacgg ctgcgccggc atgagcaacg tcgaatacgg
       61 tctcatcatg caggaacttg aacgcggcga ctccggccta cgaagcttcg tttccgttca
      121 gggcgcgctc gtgatgtacc cgattctcac ttatggcagc gagcagcaga aacaaacctg
      181 gttgccaaag ctgcaagcgg gcgaagccat cggctgcttc ggtctcacgg agccgggatt
      241 cggatcgaat ccgggcggga tgaccacgaa agccacgcgg gccggggact gttgggtgtt
      301 gaacggagag aagacctgga ttaccaacgg atcggttgcg cacatcgcgg tggtctgggc
      361 gcggacggac gaggggattc gcggcttcct cgtggaaaag ggcacggccg gctattccac
      421 agcggatatc cacgggaagc tttccatgcg cgcttccgtc acatccagcc tctcgtttca
      481 ggattgccgc atcccatccg ataacatgct gcccggcgcc aaaggtttga aggcggcctt
      541 gagttgtctt acgcaagccc gttacggcat cggatggggt gtcatcggcg cggcgatgga
      601 ttgctatgag accgcccgcc gctattcgct cgcgcggaag cagtttgacg gccgccccat
      661 cgcttcgcat caactcgtgc aggaaaagct cgcctggatg atcaccgaaa tcaccaaggc
      721 gcaattgctc gcgctgcacg tcggcaggct gaaagacgga gggaaggccg aggccgcgca
      781 catctcgatg ctcaagcgga acaatgtcgc catcgcgctc gattgcgccc gcctgagccg
      841 cgatctgctg ggtgcgaacg gcatcatgga cgaatatccc ctcatgcgcc atctgtgcaa
      901 tctcgaaacg gtcaagacgt atgaagggac ggaccatatc catgcgctcg tgatcgggga
      961 gcgggtcacg ggagtggcgg cctataaatg agcgcgcggc tgtgagcatt ctccggctcg
     1021 aacaggtaag caagaactac cagaccaacg ggacaacggt ttccgcgctg cgcgacatta
     1081 ctctggcagt gaagcctggt gaattcgtgg ccctgctggg accgagcggt tgcggaaaat
     1141 cgacgctgtt gaatctggct ggcgcgatgg acttcccgac atcgggacag gtgatccttc
     1201 gcggcatagc cacttccgcg ctcgacgatt cggcgctcac gcgcctgcgg cgcgaaacga
     1261 tcggattcgt gttccagtcg tttcaactgc tgcctacgct gacagtagcg gagaacgtgg
     1321 agttgccgtt actgctggcc ggagcgaagg gcgctcgtgc ggcggcgctg cgcgttctcg
     1381 aacaggtgga gatggacgga ttcgcatccc gcatgccgta ccagctttcg ggaggccaga
     1441 tgcagcgggt cgccatcgcg cgcgctctga ttcatacgcc tgccatcctg ctggcggacg
     1501 agccgaccgg aaatctcgac agcgcaacag gcgatctcat actggccttg ttgcggcgca
     1561 tcgcgaacac cgggcagagc accgtcatgc tcgccacgca cagcgctgaa gcagccacac
     1621 tggcggacac cgtggttcgg atgcgcgatg gccgcattca ggaaatacgc gagaacgccc
     1681 gaaaaccgta gcgcgcgatg ccgtactgga agctgttcta ccgcctcatc gtccgcccgc
     1741 tgcggcggga gcctgtccgc acgctgttga ccgctttcgc ggtggcgctc ggcgtcgcgg
     1801 tcgtattggg gatcgagttg gcgggcaccg ccgctgcggg gtcgtttcgt tcgtcgctgg
     1861 aggcgctggc cgatgacgca gatctcgaaa tcaccgccac gggcggcctt cccgccgaag
     1921 tgttggcgca agttgcgggg ctgccgcatt cgttccggtt cgaaccgcgc atggaggact
     1981 tcgcttttct tccggcacgg aatcgcaccg tgccatggat cggagtcgac ttcatcgcca
     2041 acgcaccaga gcagggcgca tctgctgggg atggtgatcc ggcagaccgg ttccgcaagg
     2101 aacaggtgtg ggtcagcgcg aacctgggat ataagaccgg cgaccggatt ccggtactga
     2161 tcggcgatac tctctcggaa ttccatgtgg gtggagtcct ctcaaagcaa cccggagaag
     2221 tgctggtggc cgacctggcc ctcacggatc gccttttccg gcgtgaagga aagctggacc
     2281 ggattctcgt acacacgcca tccggaagat ccgttgcgga gtgggagcaa ctgcttcgtt
     2341 ccgccctgcc gcccggcgcc ggcgtcgggc cgcaaggctc aagcacggac gaaaaccgcc
     2401 ggatgttggc cgcgttccgg tggaatctac gcgtgctgag ctacatcgcg ctcatcgtcg
     2461 gcgcgtttct gatctacaac accatctccg tttcggttgt gcggcggcgt gccgaaattg
     2521 gcattgtgcg ggctctcggc gccactcgcg cggcggtgct gctggggttt gtgggggagg
     2581 ccgtctgctt cggcttgtcc ggcgcgctgg cggggatcgc gctcggccgt tttctggcgg
     2641 aaagcgccgt tggactgatc gcttccaccg tgcaatctct gtacgtcagc agcactcccg
     2701 cacctgttga gctgagttgg aaaattgcgc tgctggggat cggcatgggg atcacggttg
     2761 cggtgctctc ggcgctttct ccggcgtggg aagcggcgcg cgcggctccc ctggaggcta
     2821 tggcgcgcgg gcgcggagaa caccgggtgc agatccgcaa gctgcgaggg ttgcttgcgt
     2881 cgggcctgct gtcggcgggc gcgtggatcg cttcaatgca ggacgccatg gacggaaagt
     2941 cactgttcgg ttacctggcg gcgcttctgc tgattgccgc ttcggcgttt gcgatccctg
     3001 cgctggtgtc tgtgctggct gcttcgaccg ctgcctggat gcggtgtctc ttcggcgtag
     3061 aagcgctact ggcggcgcgc gggcttgccg gatcgctccg tcgaacgtcc gtgctggtgg
     3121 gcgcattatc gacagccata gcgatgatgg ccgcggtggg catcatggtg ggcagctttc
     3181 gccagacggt cgttctgtgg atggaagacc ggcttcaggc cgacctctat ctgcgtccgg
     3241 ccgcacccgg cggcgccgac cgccatccgg tcctgtcacc ggacctggcg gaccggatcg
     3301 ccgcgctgcc cgaagtggcc gcagtggacc gcttccgtgc ttactccatc cgttatggcg
     3361 gaatgcccgc aacgcttggt gcaggggatg cgcgtattgc cggacgatat ggacgccggg
     3421 cgttcctatc cggagcaaaa ccgcaagagg tgttcgaaca attgacaggc acggatagcg
     3481 tgattgtcag cgagcctttc gcaaacaaac atggcgtgaa ggccggggac attctcagcc
     3541 ttccgattac cggacaacct gtgcgcctgc gcgttctgga tgtttactac gactactcca
     3601 gcgaacgcgg ttatatcctg atggatcgcg acaggcttct ccaatacctg ccggacccgg
     3661 cgccatcgaa catcgccgtt tacctgcgcc cggatgtgcc gctggagcag ggccgtgacg
     3721 ccatcgaacc cgtgctgaaa ggccggagag tggtggtact ttccaatcgt tcgctccggg
     3781 aagaagcact gtgggttttc gaccggacct ttgcgattac ttatgccctc gaagccgttg
     3841 ccgtgctggt ggcggtgatg ggcgttgcgg gcgcgctgct ggcgctggtc atcgaccgcc
     3901 ggcgcgaatt cgggctgttg cgctttctgg gcggcgcgca atcgcaggtg cgccgcatca
     3961 tcttgtttga agccgggctg ctcggcttgt ttgcgaatgc cgcgggtctt cttctggggt
     4021 tcttcctttc gctgcttctc atttttgtca tcaacaagca atctttcggt tggacgattc
     4081 agtttcactg gccggtgggc gtgctgatgg gcgcgctatc gctggtctac ctggcaacta
     4141 tcgccgccgc tctgtatccg gcgcgcgtgg cggcacaact gaatccgatt gaggtgattc
     4201 atgaggagta gccgcgtgtt ggccttgctg ctgctggccg cgatggcatc gttgggcgcc
     4261 gattccgact ggcgcgtggc tctgccgggc tacgcgtatt cctttccgcg agaccacttc
     4321 agccatcccg agttccgcac ggaatggtgg tactacacag gcaacctgca aacgcagaat
     4381 ggacgccgct tcggtttcga gctgaccttc ttccgtcacg cggtcgactc cacaaaaccg
     4441 gagagcgtgt gggacgttcg cgatgtctgg ctggcgcacc tggtgctgag cgacatcgac
     4501 ggcggccgat tcctccacgt ggagcggatc aacagatcgg gacccggcat cgccggcgcc
     4561 gacctgaaaa ccgcgcgcgt gtggaacggc aactggcagg tgcattggat gctcgatgca
     4621 gcccggcccg ctgccttcac gcggcaaacc ctcgatgcgg tttccgaaca atttcgaatt
     4681 catctgtcac tcgaatcgga aaagcctccc gtgattcacg gcagggatgg cgtgagccag
     4741 aaagcggagg ggcgcggccg cgcatcgcat tacatatcgc tgacgcgtct gaaggcgaac
     4801 gggtccatcg caataggagg cgaagaattc aaggtggagg ggctggcctg gatggaccac
     4861 gagttcttca cgcatcagct tgctcccaat caaaccggat gggattggct cagcctgcaa
     4921 ttcgaagacg gaacggagct gatgctgttc cgcctgcgcc gcaaagatgg ttcgatcgat
     4981 ccgtattcag cgggaacgtt tgtggacgcc ggcggcaaaa cgaagcatct cgacctgtcc
     5041 ggcttctccc tggttcccga aggaaacgtc tggcggagcc cgaaaacagg agcgcggtat
     5101 ccggtcgcct ggagcatcag cgtaccggag ctcaggctgg cggcccgggt tcgaacaagg
     5161 ctgccccaac aggaattgac cagccggcac agcggcgtgg gcgcttactg ggaaggcgcg
     5221 atcgagatca ccggaacgcg ggacggccgt ccgctcaacg gacgcgggta tctggaaatg
     5281 accggctacg ccgctcctgt gcagtagccg gttcactcgt ggcgaagggc ggccatgggg
     5341 ttgatctgcg tcgagcggcg cgcaggaatg taacccgcca acatagcaac ggccagcatc
     5401 gacacgatga cagcgccaaa ggtgatggga tcgcgaacgc ttactccgta caccaagctc
     5461 gaaaggatgc gcacggtgaa aacagcaccg atcaatccgg cgcctgttcc aatcaatgcc
     5521 aatacaaggg tctggcgcag aaccatcccc agtgcacacc gccgctgtgc tccgagcgcc
     5581 atgcgaattc cgatttccct tgtgcgcagc gtgaccgaat acgagatcaa accataaagt
     5641 ccggcagcgg cgagaagcag cccgatggct cccaacccgc cgacaatcgt cgcaaagacc
     5701 tgctgctggt aaagctgcga gcggaccagc agcttcatag tagtcacctc gaccaccggg
     5761 acgctgggat caatggctgc aacctccgct cgcaacggcc tggaaagctg aaggggatcg
     5821 agtcccgtcg tggcgataag gtacatggag gagaagttgg tttgtgcgta aggaagatag
     5881 aagtaaggct ctccagcttc catgatcgag ttgatccggg tatcacggac aatccccacg
     5941 atcgtacact cggaggccgg ggccatcctg agcgtcgaag tcgcgcccgc gcagcaggcg
     6001 gattccgagg gttcggaagt agttcaagtc aacagcagga tatttgatgc ttgaaagcgg
     6061 atctcccggt tggatggatc gcccgggaat cgtcacttgt gccgcaagac ccccgccgga
     6121 accgctcagc ggagctcgca atgcgagggt cgactcgcgg atgccgggaa tggccttcag
     6181 ccgctcgata agttgccggt agaacgcgcg attacgcggg ccgtcatacg gaggcgagat
     6241 ctcggcaacc aaaacgtccc gccgctcgaa tccgagatcg agattcatgc cgtagataaa
     6301 agttcgaacc agcagccccg cccctaccag cagcatcagg gaaagagcca tttggacgac
     6361 gacaagcgca ctgcgcgccg ggagtcgccc tcggcgtgta tccggaaggt ccccgccctt
     6421 tatcaccaac gtcagatcga tacgcgaggc gcgtaaagca ggggccagcc cgaagatgaa
     6481 aacagtcaca agggacgtga tcagcgtgaa cgcaagtacc cgctgatcca gccggaaatc
     6541 catatgaaga taactgttgg cgggcggaat gatcactgcc ggcagcagcg agacaagcca
     6601 tgcagccaga agcagtcctg ctgctgttcc caacgcgcag agcagggcgc tctcggccat
     6661 gagttgtgaa acaagacggc cgcggcttgc ccccatcgcc agacgcacgc cgatttcccg
     6721 ggagcgcccc tgggcacgtg ccagcagcag gttcgcgaga ttcgcacagg caatgagcag
     6781 cacgaccgct acgatgccga gcagaatgaa cggtacatat ttcgtcgact gaaacaggtg
     6841 ttcggtggcg gtcgccagca cggcgcggca acctttgttg aatttcggat aagcccgctc
     6901 cagattggcc gctatgcctg caatctcggc ctgcgcagct tcgatggaaa cgcccgggcg
     6961 gagcctgccc aatacttccc aattccggct ctcgcgcaag ttgcgttcgc tcggatccca
     7021 ggaagttatc ggaatccaga agtcggaatc ctgccagagt tctgtaccgc gaaactcctt
     7081 tggagcgact ccaaccacta cgtacgggcc attgaggcgc acgctccggc cgatgatccc
     7141 ggagtcgccg ccaaaacgcc gctgccagag attatgactc atgaccacga cgcgttggcc
     7201 tgtgtttgcg tctgctgcgg tgaaaacgcg gccaagctgg gcttcgacgc cgagaacgtt
     7261 gaagtagttc tcgctgacga cattcgacat ggtcgattcc gcgaagccat ctccggtaag
     7321 gatcggcccg cggcgttgtg tggcaaggag cccgctgaac gatttggcct ggctctggta
     7381 gtccagatac tcggcgtagg agagagcatc ccgcgcggag cggggagtcg gtgaaccccc
     7441 ggctcctggc aagcacccgg gccgcatagc gaaggtcgcg cgcgagattt tccacgattc
     7501 gaatgccgcg ggcctcgcgg cattgctctt tcgtctggtc gacaccgccc agcttcagcc
     7561 gggcgcggcg cgtggcttct tcttcgctca agccggtgcg catctgcttt tccacctcat
     7621 gctccaggtg aaagcgcaat tcttcatcga ggtcgtcctc catggagttc cggcgaaaca
     7681 gcgcccgcgc gcggtaaagc agatcggcaa gcatcctgtt gcgccgtttc tacgcgaagt
     7741 ccagaacctg gccgatcacc gcggccagct tgcgccaggc ttcggtttcg gccttcagtt
     7801 gcttgcggcc cgcattggtc agctcgtaat acttggcttt gcggttgttc tcgctcactc
     7861 cccacttcgc acgaatccag ccgcgccgtt cgaggcggtg caaggccgga tataaagagc
     7921 cctgacccac gcgcagcgcg tcggacgaaa tctgctggag gcgctctgtt atcgcccagc
     7981 catgctgggg gctcaacgcg agtgtcttca ggatcagaag gtccagcgtt ccctgcggta
     8041 agtccaggcg gtcgtcagcc atctcgttcc tccctttgtg cttcgacaag agaagtataa
     8101 ggcaactatt gtcgaagcgc aaggggaatc tggtaccaca tttctgtgtg aatacgatga
     8161 agaattgcaa aattttgaaa catcgtatcg ctttccttga agttcagcgt ataattgttc
     8221 gactggaaca ggtgcactat gcgaaaactg ctcatcctga cttttcccct attggtgtct
     8281 gtacacgcgt atggacagga agtccgggcc aacattacag ggcttgtgac ggacccgacg
     8341 ggggcgccag tggcggaagc caccgtaatc gtcacaaacg ttgcgcggaa tacctcggtg
     8401 caaaccgcga ccaatgagac tggtaactat ttttcacctt ttctcgtacc tggcacgtat
     8461 cgggtgagag tggaacgcgc gggcttcaag agcttcattc gagacaatat tgtgctcgaa
     8521 gcgcaggata gggcccgcgt ggatgtgcaa ctggaagtgg gagaactggc ccaaagcgtc
     8581 accgtaagcg cggacgtatc ccttctgcaa acggaaacgg ccagccgcgg gcaggtgatt
     8641 tcgacacaat tgattgaaaa cgttccgaca cagggacgga atccttttca gcttgcatgg
     8701 tctgcgccgg gggtcatcaa gacaggagac tggcgctatc tgcggccctt cgatatcggc
     8761 ggaaccagtg gtttctcgat caacggcggt gtcagaggac agaacgaggt gctgctcgac
     8821 ggcatcagca acgtgcgggg cagcggcacc gtgatccacg tccctaccat ggaggcggta
     8881 caggaattca aagtactgac gaacacctac gatgcgcaat atggccgcac ggcgggcggc
     8941 attgtgacca tcgtgaccaa atcgggcggc aacgcctttc atggaacgtt gtttgaatac
     9001 ttccaggtag aagagctcaa cgccaaccag tttgaactga atcgcgcggg cacccccaag
     9061 ccgccgatga acatcaacac gttcggattt cagctcagtg gtccggtata tgtgcccaag
     9121 gtgttcgatg gacggaaccg ccttttctgg ctgctttcgt acgaaggttt gcggcagcgc
     9181 tcggccgatc ccggcgccgc gaatttccct ctcgccgagt ggcgcaacgg tgacttctcc
     9241 acattattga acgcccaagg gcagcaggtg cgcatctacg atccgcttac gacaaatgct
     9301 tcggggcagc gggagcagtt cccgggcaac cgcattcccg cgagccgcat cgaccccatc
     9361 gcgcgcgcag taatgaacct gtatcccatg ccgaactcgc ctggggatgg tccggcacac
     9421 gtcaacaact atatctatcc atcgcgctgg gtcgccgaca tggacgagtg gatcggccgc
     9481 ctggatctga tggtgaatcc gagcaacgta gtgtttttcc ggtacgggca gaatccattc
     9541 tccgaatacc ggagcatcgt atggggcacc aatgaggcgg agcccaccgg caacgcaccc
     9601 ctgatacgca acgggcgcaa ttggaccatt gactggactt ccacgctgag ccctcacatg
     9661 accttcaacc ttcgcgcagg tttgaaccgg tgggaagagc agacgggcaa cacctacggc
     9721 gcgaactacg atccacgcca gctcgggttc gcttccagtc ttgtatccca gttcacgaaa
     9781 ctccaattcc cccggttcga cctggatacg taccaggcta tcggctccga ccgtctgctt
     9841 gcgctcgggg ctaacgatac ctacagcatt caacccaacc tcagcctggt ggtaggcacg
     9901 cacttcctga agttcggagg ggaaggccgg cgctacaacg acaacacgaa caatccgggg
     9961 ctcgccagcg gcctttacac gttccgcaag aactggacgc aggaacgcgc gctgcaaggc
    10021 gacgccgttt ccggaaacga actggcaact ttcctgcttg gctatccgac taccgcctac
    10081 gtggatcgca acatcgatcc cgccttcacg aacaactatt ttgccgcgtt cgtgcaggac
    10141 gactggaaga tcacgccacg gctgagcctg aatctcggac ttcgctggga ctacgagacg
    10201 ccaatggtgg agcggttcga ccgcatggtc cgcggcctcg attttgatgc cgccagcccg
    10261 ctggcttcgc aggcttccgg aatgaatctg aagggtgcgc tgctctttgc cggggttggc
    10321 ggacagcctc ggggttcctt tgagcccgac cgcaacaact tccagcctcg cgtcggcgcc
    10381 gctttgcgcc tcgggcagcg ctgggtggtg cgcggcggct acggccttta ctacctggga
    10441 caaaaccaga ggggatcgaa ccaggggttc agccaacgca ccaacgccat cgtctccacg
    10501 gacgggaacc tgactccggc cgtgacgctc gccaatgctt tcgcgaatct ccccggaggt
    10561 caactgctat ctgccgtcgg ggcaggcaac ggagcagcca gcttcctcgg cgagagtctt
    10621 accgtgaact accttaaccg accgctccca tatgcccatc agtattcggt ggacatcgaa
    10681 cgggaattgc ccggcaatct gctggtggac gtcgcctacg tcggcaacat cacgcgcagg
    10741 ctgcccgtga acaacgtgaa tctgaatgcg attccggctg aagaactggg ccgccgcaca
    10801 ccggcgggcg ctatcgatac cg
//
LOCUS       DASTMK010000519         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_283476, whole genome shotgun sequence.
ACCESSION   DASTMK010000519 DASTMK010000000
VERSION     DASTMK010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283476"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFQ79_21540"
     CDS             complement(<1..422)
                     /locus_tag="VFQ79_21540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0142319.1"
                     /translation="MLRTAGTAHPTTRPGQATGVPYIPVMSGLEGSRNSVCPLAAFKC
                     LSATQPTLESARSAFLNAAIAMSVIDVTGRYICVNRALCELTGYSEEELLATNFQSIV
                     YAGDLKDSLALLHRLLAGPETNAVSEMRYRRRDGGTVWV"
     gene            complement(460..2103)
                     /locus_tag="VFQ79_21545"
     CDS             complement(460..2103)
                     /locus_tag="VFQ79_21545"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0142320.1"
                     /translation="MTGISYKQHSLAEDWDAVVIGSGMGALATAALLAKHARLRVLVL
                     ERHYTAGGYTHSFSRPGFDWDVGVHYIGQVQDPNSLLRRAFDHLTEGRLEWHPMPDVY
                     DRAIIAGHEYEFVSGEQRFRERMKQYFPREGRAIDAYINAVKSSARMSGLYFAEKAIP
                     GPAARIAGALMRAPFLRWAGRTTAEVLGGLTDNRELIAVLTAQWGDYGLPPAQSSFGI
                     HAAIAHHFLEGAAYPVGGAAQIAASVAPVIEQAGGKIVVGAEVSEVLVENGKASGVRM
                     SDGKVFRAPRVISDAGAGNTLGSLLRSPAPGVERVLEEIRRLPPSMAYLTLYVGLDGT
                     AAELGLKGTNIWVHPTANHDGNLARFNEGLANPFPLLFISFPSAKDPEFEQRHPGHAT
                     VEVVTPVPYDWFRNWQDTRWKRRGSEYQEMKAGFSSRLLAELERYVPAVHGRIAHAEL
                     STPVTARHFMNHARGEAYGIAATPDRFRLRALAPRTPVPGLYLTGQDVSMPGVAGALF
                     GGALAASAVLGKNMISKMTASSRTLRGSSRRPLLAGTSS"
     gene            2194..2829
                     /locus_tag="VFQ79_21550"
     CDS             2194..2829
                     /locus_tag="VFQ79_21550"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0142321.1"
                     /translation="MKQPHPYHHGNLKEALLEAALQHIDTAGPGGITLREVARRAGVS
                     HNAPYRHFADKDELLAAVAAQGFERLTAAMNAEAAAAGTAADRLRLTGRGYVGFALEY
                     RKHFSVMFDRPPSVHSYAEYSAAAKRAFEVLMDAIKACQDAGELPAGDPAPFAFTAWS
                     LVHGIAKLAIAGQLPFRTTADVLVFTDRATQGLLEGILKKPASPRGRRLKS"
     gene            complement(2818..3324)
                     /locus_tag="VFQ79_21555"
     CDS             complement(2818..3324)
                     /locus_tag="VFQ79_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0142322.1"
                     /translation="MALKDALLPEFDYEMANTRRTLERVPEDRFDWRPHEKSPAIGWL
                     ATHLANIPSWCTYALQQDSLDLAPGGQEQHFPTAASLKEVLQNFDTNVAAARQAIGNT
                     SDEQFAVTWTLFKAGQTILSMPRVVVLRGFVLNHIVHHRGQLTVYLRLNNIPVPALYG
                     PSADESAF"
     gene            3391..4065
                     /locus_tag="VFQ79_21560"
     CDS             3391..4065
                     /locus_tag="VFQ79_21560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU0142323.1"
                     /translation="MNTGFQLFGPAHLLILAAVPSLAIVLSQAARRGLVPPRPLVFAL
                     GVFLAINELFWYAFRLRREGFRFPEGLPLHLCDLMLWLTVVAALTLNRRVFEVAWFAG
                     IGGASMALFTPDLWAPLASYPTIYFFLAHGFVVVTLLTLLGSGQAALRPGCVWRAFAI
                     VNAYAAAVGIFNLIYGTNYMYLCRKPPSASLLDYLGPWPVYVLAGEAVALAAFWLLYL
                     PVRRRS"
     gene            4309..>4590
                     /locus_tag="VFQ79_21565"
     CDS             4309..>4590
                     /locus_tag="VFQ79_21565"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0142324.1"
                     /translation="MRRFPKEPRARLGRGLYRLQMGESLSMPNARPMATIASGVWELR
                     VRGEDGIFRVFYYTTSGAGVLVFHAFAKKTQRTPASEIELARKRLKELLD"
BASE COUNT          808 a         1411 c         1447 g          924 t
ORIGIN      
        1 acccagactg tgcccccatc cctgcggcgg tagcgcattt ccgataccgc attggtttcc
       61 ggacccgcca gcagccggtg caggagtgcc aggctgtcct tcagatcacc cgcatagacg
      121 atcgactgaa aattcgttgc aagcaattcc tcttctgaat atcccgtcaa ttcgcacaac
      181 gcgcgattca cacagatata gcggcccgta acgtcgatga ccgacatcgc gatcgccgca
      241 tttaggaagg cggaacgcgc cgactccaga gtgggttgcg tcgccgacag gcacttgaag
      301 gccgccagag ggcagacgct gttgcgagat ccttccaagc cgctcataac tgggatgtag
      361 ggtacgccgg tggcctgtcc cggccgggtc gtcgggtggg ccgttcccgc cgtacgcagc
      421 aacacctcat tcctctttcg gcatgttgga aggggaagtt tagctcgagg tgccggcaag
      481 cagcggccgg cgggacgatc cgcgcagtgt ccggctgctt gccgtcattt tcgagatcat
      541 gttctttccc agcaccgccg atgcggccag cgctccccca aacagcgcgc ccgcaactcc
      601 cggcatggat acatcctgtc cggtcagata caggccggga acgggagtgc gcggtgcgag
      661 cgcgcgcaag cgaaagcgat ccggcgtcgc ggcaatgcca taggcttcgc ccctggcatg
      721 gttcatgaag tgccgcgcgg tcaccggcgt ggacagttcc gcatgggcga tacgtccgtg
      781 gacggcggga acgtaacgct ccaattccgc cagcagccgg ctggaaaaac cggctttcat
      841 ctcctggtac tcgctgcccc ggcgcttcca gcgcgtgtcc tgccagtttc gaaaccagtc
      901 gtagggcacg ggcgttacga cttcgacggt cgcatgaccg gggtgccgtt gttcgaactc
      961 cggatccttc gccgacggga aggagatgaa taaaagcggg aacgggtttg caagaccttc
     1021 gttaaaccgc gcaaggttcc cgtcgtgatt cgcggtaggg tgtacccaga tgttcgtgcc
     1081 cttcaacccc agttcggcgg cggtgccgtc caaacccacg taaagcgtca ggtaggccat
     1141 cgacggcggg agacgccgga tttcctccag aacccgctcg acgccgggcg ccggggagcg
     1201 cagcaaagaa ccgagcgtat tcccggcgcc cgcatcgctg atgaccctgg gcgcgcgaaa
     1261 caccttgccg tcactcatac ggacgccact cgctttaccg ttttctacga gcacctcgct
     1321 tacttccgcc cccaccacga tctttcctcc cgcctgctca atgaccggcg ccacggacgc
     1381 ggcgatctgg gccgccccgc ctacgggata ggctgctccc tccagaaaat ggtgggcaat
     1441 cgctgcatga atcccgaaac tgctttgagc gggcggaagt ccatagtcgc cccattgcgc
     1501 cgtcagcacc gcgatcaact cccggttatc cgtcaggccg ccgagcacct ccgccgtggt
     1561 gcgccccgcc caccggagaa agggcgcccg cataagcgct cctgcgatcc gcgcggcagg
     1621 cccgggaatc gccttttcgg cgaagtacaa cccgctcatg cgcgcgctgg acttaacggc
     1681 gttgatgtac gcatcgatcg cccggccctc gcgggggaag tattgcttca tgcgttcgcg
     1741 aaaccgttgt tcgccgctga cgaactcata ttcatggccc gcgatgatcg cacggtcgta
     1801 cacgtcgggc atgggatgcc actcgaggcg cccttccgtc aggtggtcga aggcccgccg
     1861 caacagagag ttcgggtcct gtacctggcc gatataatgc actccgacgt cccagtcgaa
     1921 tccaggccgg ctgaaagagt gcgtgtaccc gcccgccgta tagtggcgct ccagcaccag
     1981 tacgcgaagc cgggcgtgtt tggccagcag ggccgccgtg gcgagcgcgc ccatgccgga
     2041 gccgatcacc accgcgtccc agtcttccgc gagcgaatgc tgtttgtagg aaatcccggt
     2101 catgatgttt ttacctcctc taatgtggac actgacaaca ttttaaagct tgatgttgtc
     2161 aatgtcaaca cttttctgct gaaatagaga ggcatgaagc agccccatcc ctaccaccac
     2221 ggcaatctga aggaagccct gctcgaagcg gccttgcaac acattgatac ggcgggacca
     2281 ggaggcatta cgctgcggga agtggcgcgg cgtgccggtg tttcgcataa tgcgccgtat
     2341 cgccatttcg cggataaaga cgagctgctc gccgccgtgg ctgcccaggg tttcgagcgg
     2401 ttgacggccg caatgaatgc cgaagcggcg gcggccggga cggcagcgga caggctacgg
     2461 cttaccgggc gcggttatgt cggttttgcg ctggagtacc ggaagcactt ttcggtgatg
     2521 ttcgaccggc ctccgtcagt tcattcttat gcggaatact ccgccgcggc gaagcgcgcc
     2581 ttcgaagttc tgatggacgc catcaaagcc tgtcaggacg cgggggaact cccggcgggc
     2641 gaccccgctc cgtttgcctt caccgcctgg tcgctggtgc atggaatcgc gaagctcgcc
     2701 atcgccggcc agttgccttt tcgcaccacg gccgacgtgc tggtgttcac cgatcgcgcg
     2761 acacagggac ttctggaagg aatcctgaag aagccggctt cgccacgcgg acggcggcta
     2821 aaaagctgac tcgtcggcgg acggcccata cagcgccggc acggggatgt tgttcaatcg
     2881 cagatacacg gtgagttggc cccggtgatg cacaatgtga tttagcacga agccccggag
     2941 gacgaccacg cgcggcatgc tgagaatggt ctgtcccgct ttgaacaggg tccaggtaac
     3001 ggcgaactgt tcgtcgctgg tgttgccgat ggcctgccgg gcggccgcga cgttggtgtc
     3061 aaaattctgg agcacttctt tcagggaagc ggctgtgggg aagtgctgct cctggccgcc
     3121 gggtgcgagg tcgagcgagt cctgctggag ggcgtaggtg caccagcttg gaatattcgc
     3181 caggtgtgtt gcgagccatc ctatcgccgg cgacttttca tgcggccgcc aatcgaaacg
     3241 gtcttccggc acgcgttcca gggtccgccg ggtgttggcc atctcgtagt cgaattcggg
     3301 caacaatgcg tctttgagtg ccatatttgt caattctacc ggactctacc cggcggcttc
     3361 gggtacaacg aaagcaagac tgaaaaacgg gtgaatacgg gattccaact cttcggtccg
     3421 gcgcacctgc tgattctggc ggcggtgcct tcgctggcga tcgtgttgtc gcaggcggcg
     3481 cggaggggcc ttgtgcctcc gcgcccgctc gtgttcgctc tcggtgtctt tctcgcgatc
     3541 aatgaattgt tctggtatgc cttccggttg cgccgggaag gcttccggtt tcccgagggg
     3601 ctgccgcttc atctctgcga tctcatgctg tggctgaccg tggtcgccgc gctgacgttg
     3661 aaccggcggg tcttcgaggt ggcctggttc gccggcatcg gcggtgcttc catggctctg
     3721 ttcacgccgg acctctgggc gccgctggcg tcctacccga ctatctattt cttcctggcg
     3781 cacgggttcg tcgtcgtcac gctgctgacg ctgctcggga gcggccaggc ggcgcttcga
     3841 ccgggctgcg tctggagggc ctttgccatc gtgaacgcct atgcggccgc ggttggaatt
     3901 ttcaacctga tctacgggac aaactacatg tacctgtgcc ggaagccgcc cagcgcttcg
     3961 ctgctcgact atcttggtcc gtggcccgtg tatgtgctgg ccggcgaagc cgtggcgctg
     4021 gcggcgttct ggctgctgta tttgccggtg cggcggcgga gctagcgaac ggcccgttgg
     4081 gaccgccgca ctgcggacgg ggtactgaat actggggaca ttcggcagct tgtatcgccc
     4141 tgggagttcc gcgatttggg cgtgatacgg ccaagtgctt cgctttagac aagccgactt
     4201 ctcgggagta atctggcggt ccggagtacc gggctacacc gtatgtctca aacctgatac
     4261 atttagaggg cgcggccggt tatcttccat ccgaaggcgc gggatgagat ccgccggttt
     4321 cccaaggaac cgcgagcccg tctcgggcgg ggcctgtatc ggttgcagat gggggaatca
     4381 ttgagcatgc cgaacgcacg gccgatggca accatagctt cgggcgtttg ggaattgcgc
     4441 gtgagaggcg aggatggaat tttccgggtg ttctattaca cgacatccgg ggccggcgtc
     4501 ctggttttcc atgcgttcgc aaagaagacc cagcggactc cggcatcgga gatcgaactg
     4561 gcgagaaaac gtttgaagga gcttctcgat
//
LOCUS       DASTMK010000520         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_283533, whole genome shotgun sequence.
ACCESSION   DASTMK010000520 DASTMK010000000
VERSION     DASTMK010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283533"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFQ79_21570"
     CDS             complement(<1..205)
                     /locus_tag="VFQ79_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142325.1"
                     /translation="MVQIDVPIALGVGSLFADAAHRQLSTGRPEYYYRTFAANNIFQT
                     FFFSWIPLYFLLNYFGWETTHMWW"
     gene            complement(293..2965)
                     /locus_tag="VFQ79_21575"
     CDS             complement(293..2965)
                     /locus_tag="VFQ79_21575"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0142326.1"
                     /translation="MSDDSGLTKREFLQTAGTLPALKLMLGSAAAAAPETGASKFTSV
                     PLAEYFNAAPRDLGSKPEARGLSAGGRQDGLLRMPAGSQTFRGIPFLLGPEGVANKSW
                     VALSRSQRPWATVEVEIAAAGKAGYLCLAGFCDWDPLESPAPGDLAAERVGQQLAELV
                     FLFEDGTRQSAPIRRRFETNSPSYRWGHLSFASVPHRQNLPRSLSDPLRDARHWGDLQ
                     MGLWEGAYGGNPAVDNPATLWLAAIGNPSPDRAIKGLRFEARSDDLLMLCGLTLFHGR
                     QNPLRYDRLALYRITPPVKGGTPGGNRRWSVSVDLGVVARTWQAGEFDTASWLDSPTK
                     GLPERTGPVAADAPLYAEVSASPDATLTLTNTETGEKYDLPLAGAEAPSSSVSVRVLE
                     REKTWLHGTILDSGTKRPTPVRLAFRSKEGRYIPPYGHRTEINGGWFQDYGADLRWSD
                     SSFAYVDGTFQVELPAGEVYVELSKGFEYEPVRRKLDIAAGQRELTLEIGRFADLRSK
                     NWVSADTHVHFLSPSTAVLEAQAEGLNLINLLAAQWGDLFTNVGDLSYGPLTSKDGEA
                     MVHVGTENRQHILGHLGLLGGHGQPVFPMSASGPHESYFGDPLWTTLSDWADACRKRE
                     GLVVAVHFPYPTAELAAAIEGKRIDAVELRPTGMTEHFNNLYFQEWYRYLNCGYRLPI
                     AGGTDKMSASVPAGGYRTYAHLGQDEFTFANWAKAVRAGKTFLSSGPLLQFSADGQAP
                     GAEIRTSGPASIEVIASADSSTPIHRMDIVWNGTVVASRENGAGARNLTLKETIRTNG
                     TGWLAARCASKTVAGGIRVAAHTSPVYVVAPGQELFSRDTAAYMLTLIEGAELWASTL
                     ATRPDAERFEKVLGVFRDARASLHQRLHKHGIRH"
     gene            3109..3291
                     /locus_tag="VFQ79_21580"
     CDS             3109..3291
                     /locus_tag="VFQ79_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142327.1"
                     /translation="MEDNNDDILNSLSKLMERESPGKTAEEWLVYLKDHMEHQKPLPV
                     ETSDLINLARAIDRSY"
     gene            3362..>3560
                     /locus_tag="VFQ79_21585"
     CDS             3362..>3560
                     /locus_tag="VFQ79_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0142328.1"
                     /translation="MSLAGKVAVVTGAARGIGWATAAQLASDGARIVLCDIRPPEGRD
                     MRDGAFEIHGQPALFVQADVGG"
BASE COUNT          749 a         1058 c         1058 g          695 t
ORIGIN      
        1 gccaccacat gtgcgttgtt tcccatccga aataattcag caggaagtag agcggaatcc
       61 aactgaagaa gaacgtctgg aaaatgttgt tcgcagcgaa cgtccggtag taatactccg
      121 gtctcccggt gctgagttgg cggtgcgccg cgtcggcaaa gagactaccg acaccaagcg
      181 cgatagggac atcaatctga accatacggg gcagcagccc ttcccaggat tctgaaatta
      241 tcgctccggg agggcgttgt caatagtgaa agtaccggat gatccacttt ctctagtgcc
      301 ggatgccgtg tttatgcagc cgctggtgaa gcgacgcccg tgcatcccgg aacacgccca
      361 gcactttctc aaaacgctcc gcgtccggac gagtggcaag agtggaggcc catagttccg
      421 cgccttcgat gagtgtcaac atgtacgccg ccgtatcgcg cgaaaacagt tcctgtccgg
      481 gagcaacgac ataaacgggc gaagtatggg cggcaacgcg aatcccaccg gcaacggtct
      541 tggaggcaca acgggccgcc agccagcccg tgccattggt gcgaatagtt tccttgagcg
      601 tgagattgcg cgccccagct ccgttttcgc gcgaggccac cactgtcccg ttccagacaa
      661 tatccatccg atgaatagga gtgctcgaat ccgcggatgc gatcacctcg atggacgccg
      721 gaccggaggt ccggatctcc gcgccgggcg cctgtccgtc cgcgctgaac tgcaaaagag
      781 ggcccgagga gagaaaggtc ttacccgcgc gaacggcctt cgcccaattg gcgaacgtga
      841 actcgtcctg gccaagatga gcgtaggttc gatatcctcc ggcgggaacc gatgcgctca
      901 tcttgtccgt accaccggcg atgggcaggc ggtacccgca gttcaggtag cggtaccatt
      961 cctggaaata caggttgttg aagtgctcgg tcatccccgt aggccgcagt tcgacggcat
     1021 cgattcgttt cccctcaatc gccgcggcga gctcagccgt cggataggga aaatgaaccg
     1081 caaccaccag gccttcgcgc ttccggcacg catcggccca atccgatagc gtggtccaca
     1141 acggatcgcc aaaatagctt tcatggggac cggaggcgga catcgggaac acgggctgcc
     1201 cgtgcccgcc cagcagcccc aggtgtccca gaatgtgctg ccggttctct gtgccaacat
     1261 gaaccatcgc ctcgccgtct ttcgacgtta aggggccgta cgagagatcg ccgacattgg
     1321 tgaacagatc gccccattgc gccgccagca gattgatgag gttgagccct tcggcctgcg
     1381 cttccagaac agcggtgctc ggcgagagaa aatgaacgtg cgtatccgca ctcacccagt
     1441 tcttcgatcg taggtcggcg aagcggccga tctcgagtgt aagctcgcgc tgtccggcgg
     1501 cgatgtccag ctttctacga accggttcgt actcgaagcc tttggagagc tcgacgtaaa
     1561 cctccccggc cggcaattcc acctggaaag ttccgtcgac gtatgcaaaa gaactgtcac
     1621 tccatcggag gtcggcgcca taatcctgaa accagccgcc gttgatttcg gtgcgatgtc
     1681 cgtagggagg aatgtagcgc ccctctttcg agcggaacgc aaggcggacg ggtgtcggcc
     1741 gcttcgttcc ggaatcgagg atggtaccgt gcagccatgt tttctcgcgc tcaagcacac
     1801 gcacgctcac gctgctcgaa ggcgcctctg ctccggccag cggaaggtcg tacttctcac
     1861 cagtttcggt attggtgagc gtaagcgtgg cgtccgggct cgccgaaacc tcggcgtaga
     1921 gaggcgcgtc cgccgcaacc ggtccagtgc gctcggggag ccccttcgtg ggagagtcga
     1981 gccacgacgc cgtatcgaat tcccctgcct gccacgtgcg ggcgactacg ccaagatcaa
     2041 cggaaacgct ccatcggcga ttccctccag gggtcccgcc tttcaccggc ggcgtgatgc
     2101 ggtatagagc cagacgatcg taccgcagcg gattctgccg gccatgaaaa agcgtcagac
     2161 cgcagagcat cagcagatcg tccgagcggg cttcgaaccg cagccctttt attgcccggt
     2221 ctggagaagg attcccgatg gccgcgagcc acaaagtggc tggattatcg actgcgggat
     2281 ttcctccgta cgctccttcc catagaccca tctggagatc gccccaatgc cgtgcgtctc
     2341 gaagtgggtc ggaaagactt cgcggcaggt tctgccgatg tggaacggaa gcgaagctca
     2401 gatgtcccca tcggtaactg ggtgagttcg tttcgaaacg gcgccggatc ggcgctgact
     2461 ggcgtgtacc atcttcgaac aggaacacca actcggcgag ttgctggcca acccgttcgg
     2521 ccgccaaatc cccaggcgcg ggcgattcga gtggatccca atcgcagaat ccggcgaggc
     2581 acaaatagcc ggcctttccg gcggccgcga tctcgacttc caccgtcgcc catggtcttt
     2641 gcgaccggct gagcgccacc caggatttgt tcgcgactcc ttccgggccg agcagaaatg
     2701 gaattccacg aaacgtttgc gaacccgccg gcattcgtaa gagcccatcc tggcggcctc
     2761 cggcgctgag gcccctcgcc tccggcttcg agcccagatc gcgcggggca gcgttgaaat
     2821 attccgcaag aggtacagat gtaaacttgg atgccccggt ttcaggtgca gcggccgcgg
     2881 cagatccgag cattaatttc aaggcaggca acgttcccgc agtctgcagg aactctcgtt
     2941 ttgtcaaccc agaatcatcg gacatgttga attccccaac gaagcttatc atttcccgat
     3001 atgtgagttt gcacgcggaa aacacgtccc atttagggac aaattcgatg ccgcatccga
     3061 tgagagccgg ggctcatcca gcttacttgg aatctagagg cggactctat ggaagacaac
     3121 aatgacgata ttttaaacag cctcagcaag ctgatggagc gggagagtcc cggcaaaaca
     3181 gcggaagaat ggcttgttta cctgaaagat cacatggagc atcaaaagcc tcttccagtc
     3241 gaaacgtcag acctgattaa tcttgcgcgg gccatcgaca gatcctactg aatagaagtt
     3301 gtttcttttc ggatgacgcc gctcctgctt actcctggta atcttgatgc gaggtgcgcg
     3361 tgtgagtctg gcaggaaagg tggcggtagt taccggagcg gcgcggggaa tcgggtgggc
     3421 cactgcggcc cagttggctt cagatggggc gcgaatcgtg ttgtgcgata tccggcctcc
     3481 ggaaggacgg gatatgcgcg acggcgcgtt tgaaatacat gggcagcctg cactctttgt
     3541 acaggcggat gtcgggggtc
//
LOCUS       DASTMK010000521         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_123106, whole genome shotgun sequence.
ACCESSION   DASTMK010000521 DASTMK010000000
VERSION     DASTMK010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123106"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2386)
                     /locus_tag="VFQ79_21590"
     CDS             complement(20..2386)
                     /locus_tag="VFQ79_21590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0142329.1"
                     /translation="MRIRSSFPIVVLLCGVAAAQNQTQTRQQVLEELQQILLPSRTPP
                     TGRINATDKTWEDWVKRTGELPPDFDSMPSLAELPDPLMLDEDGRQVPVTTPEQWLRK
                     KQWIRGQVERWMFGTMPPRPDNLRSRVTAERREGTSTVRDVMLEFGPEHRGKLRVQLI
                     IPHGKGPFPVFLTNHARNKPWLYTAVNRGYIACVYYATDPRYENGDDSDAFIELYPEY
                     DFSCLGRWAWSASRAVDYLERLPEVDKNKIGLTGHSRNGKQALLAAAFDERIGAVIPS
                     SGNTGEIIPWRFNTNPYAVESLELLTGAQSHWFHPRLHFFAGREHKLPVDQNLLLAMV
                     APRGLMMYSGYAESAANPLAFEQAYRSALRAYQFLGHPEKIWLHLREGEHATTAEDIE
                     TFIDFFDAVFERKERRKSETWIHGYDFDTWREGTGEKMDASRFPERAPEDFAPASRAE
                     WESQKQKIREALKAVMGPEAPHVPFEAKKRLGGMIWAGEGWLATLFGRPTDERHWQQR
                     LAASGMGVATVPFGDGLAGKLFYPVDADGKPKPGPWPVAVWLHPYAYSTGWSARSPWN
                     PTATDYLADQRPSFASLVRRGVAVFAFDQIGFGSRLHQAQRFYERYPQWSLMGKMVKD
                     TRDAIEALAAMEEIDASRISLFGYSLGAKVGLLTAALDERVQSVAAVCGVEALRLNSP
                     GTEGLYHYSHIHGLLPRLGFFIGHEPRAPFDYDEIMALAAPRPVLVVAPTRDRYAPVE
                     DVRREVEASRRVYRLMGRNEALEFETPTDINRFSRATQERVFDWLAAR"
     gene            2531..3232
                     /locus_tag="VFQ79_21595"
     CDS             2531..3232
                     /locus_tag="VFQ79_21595"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEU0142330.1"
                     /translation="MAITVVGLFENRDHAERAVMALMDAGLSRDEISVVSKDERGAES
                     PDLTPKPGVGTAAGAGAGAAIGGMAGIVAGVAALAIPGIGPILAAGPVATALMGGAVG
                     AAAGGLIGGLSSMGVDEEDARTYSEAVGRGHALVAVRATPADADRAADILENYGAFDV
                     DERVELWRTGGAGATLGKTRAAGSTLSAGAAGTPQASLERPTGLSPDEFQEKRRRRAV
                     RSYPVSDTYHGEGEV"
BASE COUNT          628 a         1034 c         1035 g          662 t
ORIGIN      
        1 taccggacag tgattccggc taccgcgccg ccagccagtc gaatacgcgc tcctgcgtag
       61 cgcgcgaaaa acggttgatg tcggtcggcg tctcaaattc caaagcctcg ttccgcccca
      121 tcaaccggta gacgcggcgc gatgcttcta cttcgcgtct tacgtcttcc acaggcgcgt
      181 agcggtcacg ggtgggggcg acaaccagca ccgggcgcgg tgctgccagc gccatgatct
      241 cgtcataatc gaacggcgcg cgcggctcat gcccaatgaa gaagcccagc cgcggtaata
      301 agccgtggat atgcgagtag tggtaaagtc cctccgtccc ggggctattc aagcgcagcg
      361 cttccactcc acacaccgcc gccaccgact gcactctttc atcgagggcg gccgtaagca
      421 gtccgacctt tgcgcccagc gaatagccga acaggctgat gcgggatgcg tcgatttctt
      481 ccatggcggc gagtgcctcg atggcatcgc gcgtgtcttt caccatcttg cccatcaacg
      541 accattgcgg ataccgctcg tagaacctct gcgcctggtg cagccttgat ccgaagccga
      601 tctgatcgaa tgcgaacacg gcgacgccgc gccgcaccag agaggcgaac gacggacgct
      661 gatccgccag gtagtccgtt gcagtcgggt tccacggcga gcgtgcggac cagcctgtcg
      721 agtaagcgta cgggtgcagc cataccgcta ccggccatgg ccccggtttg ggttttccgt
      781 ccgcgtcgac cggataaaac aattttcccg ccagcccatc gccgaatggg actgtagcga
      841 cacccatacc cgaagccgcc agccgctgct gccagtgccg ttcatccgtc ggccgcccga
      901 acagcgtggc cagccatcct tctccggccc agatcatgcc gcccaggcgc ttcttcgcct
      961 caaagggcac gtgcggcgct tccgggccca tgactgcttt aagggcctcg cggatcttct
     1021 gcttttgcga ttcccattcg gcgcgtgagg ctggtgcgaa atcttctgga gcccgctccg
     1081 ggaaccggga agcatccatc ttctctccgg tgccctcgcg ccaggtgtcg aaatcgtagc
     1141 cgtgaatcca ggtctccgat ttccgcctct ctttcctttc aaacacggcg tcgaagaaat
     1201 cgataaaagt ttcgatgtcc tcggcggttg ttgcatgctc gccttcccgc aggtgcagcc
     1261 agattttttc cggatgcccc aggaactgat acgcgcgcag cgcggagcgg tatgcctgct
     1321 cgaatgcgag cggattcgcc gccgattccg cgtaccccga atacatcatc aagccacggg
     1381 gcgccaccat cgccaggagc agattctggt ccaccggcag cttgtgctcg cggccggcaa
     1441 agaaatgcag gcgcggatga aaccagtgcg attgtgcgcc ggtcagcagt tcgaggcttt
     1501 ccacggcgta aggatttgtg ttgaagcgcc acggaatgat ttcgccggtg ttgccgctcg
     1561 atggaatcac ggcgccgatc cgctcatcga acgccgccgc cagcagcgcc tgtttcccgt
     1621 tgcgggaatg cccggtcaaa ccgattttgt tcttgtccac ttccggcagc cgctccaggt
     1681 agtccaccgc gcgcgacgcg gaccacgccc accgccccag gcacgaaaaa tcatactccg
     1741 gatacagttc gatgaaggca tccgaatcgt cgccgttctc gtagcgcgga tcggtggcat
     1801 agtagacgca cgcgatatag ccgcgattca cggcggtata gagccatggt ttgttgcgcg
     1861 catgattcgt caggaagacg ggaaatggtc ccttgccgtg gggaatgatg agctgaacgc
     1921 gcagcttgcc ccgatgctcc ggaccaaact ccagcatcac gtcgcgcaca gtggaggttc
     1981 cttcgcggcg ctcggccgtc accctggatc ggagattatc gggccggggc ggcatggttc
     2041 cgaacatcca tcgctccacc tgacctcgaa tccattgttt ctttcgcaac cactgttcgg
     2101 gtgtggtgac cggaacctgc cgtccatctt catcgagcat caacgggtcc ggcaactccg
     2161 cgagcgatgg catcgaatcg aagtccggcg gcaattcgcc tgtcctcttg acccagtcct
     2221 cccaggtctt gtcggtggca ttgatccgtc cggtcggcgg cgtgcggctg ggcaatagaa
     2281 tctgttgcag ctcttccagg acctgttgcc gggtctgtgt ctggttctgt gccgccgcca
     2341 caccgcagag taataccacg atgggaaaag acgatcggat tcgcatggta tccactcagc
     2401 ttactccgaa cgaagcggga ccgaatggag aatttaccgg gctgccgttc acgcccatcc
     2461 acaccgcttt cgccaactct ttaaaaccaa aagagttggc gcctggcaac ccgattgccc
     2521 atggaaggtc atggctatca ctgtcgttgg attgtttgaa aatcgagacc atgcggagcg
     2581 tgctgtgatg gcgctgatgg atgccggact gtcccgggat gagatcagcg tggtatcgaa
     2641 agacgagcgg ggcgctgaat ctccggatct gacgcccaag cccggcgtgg gcacggctgc
     2701 gggtgcgggt gccggagcgg cgatcggtgg aatggctggg attgtggctg gcgtggccgc
     2761 actggcgatt ccgggtatcg ggccgattct ggcagccgga cctgtggcaa ctgcgttaat
     2821 gggtggggcg gtaggcgccg ctgcaggcgg attgatagga ggactgtcct cgatgggcgt
     2881 ggacgaagaa gacgcccgaa cctactcgga agccgtgggc cggggccacg cgctggttgc
     2941 cgtcagagcc actccggccg atgcggatcg cgccgccgat attctggaaa actacggggc
     3001 cttcgacgtg gatgagcggg tggaattgtg gcgtacaggg ggtgcgggag caacgttggg
     3061 caagacacgg gcggccggat cgacgctatc tgctggagct gccgggacgc cgcaggcaag
     3121 cttagagcgt cccacggggc tcagtcctga cgaatttcaa gagaagcgcc gccgccgcgc
     3181 cgtacgaagc tacccggtat ccgatactta tcatggcgag ggggaagttt agcctccagc
     3241 gtgtgtcgtg gctcggaaac gagtactgag ccgcgactgc gagggagcgg atgcgcaatc
     3301 atttccgacg ccacgtttag ttattgcttt acggcgtcct tcagtgtgaa gactacctc
//
LOCUS       DASTMK010000522         6233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_154385, whole genome shotgun sequence.
ACCESSION   DASTMK010000522 DASTMK010000000
VERSION     DASTMK010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154385"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VFQ79_21600"
     CDS             complement(<1..950)
                     /locus_tag="VFQ79_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU0142331.1"
                     /translation="MKLETLAVHAGDRKKPGPQVPVTTPIHTATSYFYDTTEQLDRIF
                     ALEESGYCYSRYDNPTNAALEELVTALEGGAGALACASGMAAVQVALFTALMDRRKSI
                     LAADALYGATVRMLMSVFEPLGFDVGFVDICDSAAVERAVAERRPGCLLMETISNPVL
                     RVGAMDRVAEIARAAGAALVVDNTFATPLLVRPLELGASLVVHSATKYLAGHGDVLGG
                     VVVSGAEDLEALRALSRTCGPVLGPFESYLTMRGIKTFPLRMERQCANACRLAGWLAS
                     HPSVERVYFPADPAHPDSAAIRKLFPQGMYGAMVGFELKDA"
     gene            complement(947..2347)
                     /gene="cysS"
                     /locus_tag="VFQ79_21605"
     CDS             complement(947..2347)
                     /gene="cysS"
                     /locus_tag="VFQ79_21605"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU0142332.1"
                     /translation="MALRFYNTLSQQVEEFHPLEDNIVRMYTCGPTVYNYAHIGNLRT
                     FTFEDLLRRWLRYRGYRLDHVMNITDVEDKIIRSAMAQGKSIQEYTAVYEKAFFEDMA
                     KLRLEHPERIARATEHISDMVAAVERLSEKSYTYKSDGSTYFRIAKFPGYGKLSHNDF
                     SGIRAGARVDVDEYDKADARDFVLWKARKEDEPAWETPLGPGRPGWHIECSVMSMKYL
                     GETLDIHAGGVDLIFPHHENEIAQSESLTGKQFVRYWLHAEHLLVESQKMSKSLGNIY
                     TLGELMEQGYHPEAIRYLLASVPYRKKLNFTFDGLKSAATSIERLRNFNTRLETENFP
                     EGVGAGADERAARALNEFENGMDDDLNTAAALAAVFEYIRDTNSTMDAGGFRRGDVAS
                     ARDVMQRFDSVFDVLRPSEQAGGIPDAEIEALIEERIQAKKSRNFARADQIRAELVER
                     GIVLEDTKEGTRWKRK"
     gene            complement(2400..3140)
                     /locus_tag="VFQ79_21610"
     CDS             complement(2400..3140)
                     /locus_tag="VFQ79_21610"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU0142333.1"
                     /translation="MNRSLCVFLFLVWGAQAQPASAVYSTANRPRALALIGDRYHSPV
                     YIRDGLIQAFVRENIPVTFIEEVSQLNAASLEDHQLLVILRDGMNWPQGYDKPHVKWM
                     TEEQQKAIWDFVERGGGFLALHNSQGLYPPGGLYYKLFGGDYGGHPEPYVFTIRVENR
                     DHPVTAGIEDFEIFDEQHMVKYFLDREHLLLRSMARNNAAAAAGWWREQGKGRFVYLS
                     PGHTPEALNHPMMQRLIRNSANWLLRLK"
     gene            3167..4324
                     /gene="tgt"
                     /locus_tag="VFQ79_21615"
     CDS             3167..4324
                     /gene="tgt"
                     /locus_tag="VFQ79_21615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU0142334.1"
                     /translation="MPVIRFEIEAECPVTGARAGLLHTPHGTVETPVFMPVGTQATVK
                     GLTPRDLADETGARILLANTYHLYLRPGHETVRHLGGLHGFMSWPHAILTDSGGFQVF
                     SLDKLRSVSEEGVLFRSHLNGDAHLFTPQSTVDVQLALGSDIAMVLDECLPYPVSHEA
                     ARESMERTVRWAQAAFLHYGARLGREEIRSSLFPIVQGSMFADLRVRCAEDLLGLGAE
                     GYAIGGLSVGESRTLSLEMVEATVPLLPREKPRYVMGVGMPAELPEYVARGVDMMDCV
                     LPSRNARNGYLFTSEGRVIIKQARYKEDERPVDPACHCYTCHNFSRAYLRHLFQAGEI
                     LYSMLATLHNVKHYLDIMRLTRQTILLGTFPEFLRAARSKPAEEQSDPFVR"
     gene            4384..4713
                     /gene="yajC"
                     /locus_tag="VFQ79_21620"
     CDS             4384..4713
                     /gene="yajC"
                     /locus_tag="VFQ79_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU0142335.1"
                     /translation="MSAVYLTFLLLQGQSPAGNPLLGFLPILLIFAIFYFLLFLPMQR
                     QKKNQQRLLKELQNGNVVVTTGGIVGTIVAMNEDDTLVLRVKPDNVKIQVARSAVSGL
                     LPEQVKK"
     gene            4742..>6233
                     /gene="secD"
                     /locus_tag="VFQ79_21625"
     CDS             4742..>6233
                     /gene="secD"
                     /locus_tag="VFQ79_21625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU0142336.1"
                     /translation="MIKNLKVKAVIILAVILLCIYGIIGFPTSKAKLAENLSENIRLG
                     LDLKGGSHLVLQVQVQDAFKVEADQAIERLKEALGRAGVQYASLDRSDPTSIQEADQI
                     QINIKGVQTVQTGTLRSTVAESLPSWNLAPVSSTDYRLTLKQTEALKLRQDTVTQSIH
                     TIERRINGLGLAESAVQQRGRADAEGEILVQLPGVDDPARIRQILQTTAMLELYEVKD
                     GPFGTQQDALAKHGGVLPLNTKLVRATPRGESGDGWYLLTRTPVITGRDLRDSRAAQG
                     EFGRWETTFVLTQDAARRFGRFTEQNIGNRLAIVLDNQVLSAPTIQNRIEDNGRITGA
                     ASQQDAADLALNLRAGSLPAGIVPLQEQTVGPSLGADSIRQGIIAGLVGLFVIIAIML
                     LYYRGSGVNATLALILNAVMLIAAMAWLGATMTLPGIAGVILTIGMAVDSNILIFERI
                     REELRAGKAVVAAMEAGFSRAFLTIIDTHVTTVISCAFLFIFGSGPV"
BASE COUNT         1242 a         1889 c         1828 g         1274 t
ORIGIN      
        1 gcgtctttga gctcaaaacc gaccatcgcg ccgtacatcc cttgagggaa caatttccgg
       61 atggccgctg aatccggatg tgcggggtcc gccggaaagt atacgcgctc caccgaagga
      121 tgcgatgcca gccaccccgc caggcggcac gcgttcgcac actgccgctc catacggaga
      181 gggaaggtct tgatgccgcg catcgtgagg tagctctcaa agggccccag caccgggccg
      241 caggtccgcg aaagtgcccg caacgcctcc aggtcttctg ccccggagac cacgactccg
      301 ccaagcacat ctccatgccc ggcaagatac ttcgttgcac tgtgtaccac gaggctggcg
      361 cccaattcga gcgggcgcac cagcagcgga gtggcaaagg tgttatccac caccagggcg
      421 gcgcctgccg cacgagcgat ctccgcgacg cggtccatcg cgccgacgcg caacaccggg
      481 ttcgatatcg tttccatcag aaggcagccc ggcctccgtt ccgctacggc tcgttccacc
      541 gcggcggaat cgcatatatc cacgaatccc acatcaaagc caaggggctc gaaaacactc
      601 atgagcattc gcacggtcgc cccgtacaga gcatccgccg cgagaattga ctttctgcgg
      661 tccatcaggg cggtgaagag cgcgacctgc acggccgcca tccccgaggc gcaggccagc
      721 gcgcccgcac cgccttcgag cgccgttacc aactcctcca gcgccgcgtt ggtggggttg
      781 tcgtagcgcg aatagcaata cccgctctct tccagagcaa agatacgatc cagttgttcg
      841 gtggtgtcgt agaaatacga ggtggcggtg tgaatcggag tcgtcaccgg cacctgcgga
      901 cccggcttct tgcgatcgcc cgcgtgcacc gccagtgttt caagcttcat tttcttttcc
      961 agcgtgtccc ctctttcgtg tcctcgagca caataccccg ttcaacgagt tcggcgcgga
     1021 tctggtctgc ccgtgcgaag ttccggctct tctttgcctg gatcctctct tcgatcaaag
     1081 cctcaatctc ggcgtccggg atgcctccgg cctgttcact ggggcgcagc acatcaaaga
     1141 cggaatcgaa acgttgcatc acgtcccgcg cggacgccac atcccccctc cgaaatccgc
     1201 cggcatccat cgtgctgttg gtatcgcgga tgtactcgaa cacagcggcc agggcggcgg
     1261 ccgtgttcag gtcgtcgtcc atgccgtttt cgaattcgtt cagggctcgc gccgcgcgtt
     1321 cgtccgcacc ggcgccaacg ccctcgggaa aattctccgt ttccaaacgc gtattgaaat
     1381 tccggaggcg ctcaatggac gtggcggcgg atttaagacc atcgaacgtg aagttcagct
     1441 tcttccggta tggtactgag gccagcagat atcgtatcgc ctccgggtga tatccctgtt
     1501 ccatcaactc accgagcgta tagatgttgc ccagcgattt cgacatcttc tgggattcca
     1561 ccagcaggtg ttcggcgtgc agccagtagc ggacaaactg ctttcccgtc agggattccg
     1621 attgcgcgat ttcgttttca tggtgtggga aaatcaggtc cacgccgccc gcatgaatgt
     1681 ccagcgtctc acccagatac ttcatcgaca tcaccgaaca ctcgatgtgc cagccgggcc
     1741 gcccgggtcc gagcggagtt tcccaggcag gctcgtcctc cttgcgcgct ttccacagaa
     1801 cgaagtcgcg cgcgtcggcc ttgtcgtatt cgtccacgtc cacgcgcgcg ccggcgcgaa
     1861 ttccgctgaa atcgttgtgc gaaagcttac catagccggg aaactttgca atccggaaat
     1921 aggtggagcc gtcgcttttg tacgtgtaac tcttttccga caaccgttcg accgccgcga
     1981 ccatgtcgga gatatgctcc gtagcgcgcg caatccgctc cggatgctcc agccggagct
     2041 tggccatgtc ctcaaaaaag gccttctcat aaacggcggt gtactcctgg atcgatttac
     2101 cctgtgccat cgcgctgcgg atgatcttgt cttccacatc cgtgatgttc atgacatgat
     2161 cgagccggta gccgcgataa cgcagccacc ttcgcagcag gtcctcgaag gtgaaggtac
     2221 ggagatttcc tatgtgggca tagttataaa ccgtcggccc gcaggtgtac atgcggacga
     2281 tattgtcttc aaggggatgg aattcctcca cctgctgcga gagtgtgttg tagaagcgca
     2341 aagccataaa gaagggaata ttacatcgta tcaggggaca ttcacccgga gaacgctggt
     2401 cacttcaacc gcagcagcca gttggccgag ttgcgaatca gccgctgcat catgggatgg
     2461 ttgagcgctt ccggcgtgtg ccccggcgag aggtagacaa accggccttt gccctgctcc
     2521 cgccaccaac cggccgcggc ggcggcgttg ttgcgtgcca tggatcgcag cagcaggtgt
     2581 tcgcgatcca gaaaatactt caccatgtgc tgctcatcga aaatctcgaa atcctcgatg
     2641 cccgcggtca ccggatggtc tctgttttcc acccggatgg tgaatacgta gggttccgga
     2701 tgcccgccat aatcgccgcc gaacagcttg tagtacagac cgccgggagg gtaaaggccc
     2761 tgcgaattgt gcagcgcgag aaagcctccg ccacgctcga caaagtccca gatggccttc
     2821 tgctgctcct ccgtcatcca cttcacgtgc ggcttgtcgt agccctgggg ccagttcatt
     2881 ccgtcgcgca gaatcaccag cagttgatga tcttccaacg aagcggcgtt gagttggctc
     2941 acctcctcga taaacgtcac ggggatgttc tcccgcacga acgcctggat gagcccatcc
     3001 cggatgtaga ccgggctatg ataccggtcg ccgatgagcg ccagcgcccg ggggcggttc
     3061 gccgtgctgt aaacagccga tgccggctgg gcctgtgcgc cccacaccag aaacaaaaac
     3121 acacacagag agcggttcat atgcggtgca attctacaat agaacggtgc cggtaatccg
     3181 ttttgaaatt gaggcagaat gtcccgtcac gggggcgcgc gccgggttgc ttcatacccc
     3241 gcacggaact gtggagacgc ccgtgttcat gccggtcggc acgcaggcga ccgtgaaggg
     3301 gctgacaccg agggacctgg cggacgaaac gggcgcacgg atcctcctcg cgaacaccta
     3361 tcacctgtac ctgcgtccgg gccacgaaac ggttcgccat ctcggcggct tacacggctt
     3421 catgtcctgg ccgcacgcga ttttgacgga ttcgggcggg ttccaggttt tcagtctcga
     3481 caagctgagg agcgtcagtg aggagggcgt gctcttccgt tcgcatctga acggcgatgc
     3541 acatcttttt acgccgcagt cgacggtgga cgtgcagttg gccttgggga gcgatattgc
     3601 gatggtgctg gatgaatgcc tcccctaccc ggtcagccac gaggctgccc gtgagtccat
     3661 ggaacggacc gtgcggtggg cgcaagcggc tttcctgcat tacggcgccc ggctcgggcg
     3721 ggaagagata cgctcgtccc tgttccctat tgtgcagggg tcgatgtttg cggatcttcg
     3781 cgtccggtgc gcggaggacc tgctggggct cggcgccgag gggtacgcca ttggcggctt
     3841 atcggttgga gagtcgcgca cgctgagcct ggagatggtg gaagctacag ttccgttact
     3901 gccgcgcgag aaaccgcgct atgtcatggg tgttggaatg ccggccgaac tgcccgagta
     3961 tgtggcccgc ggggtggaca tgatggactg cgtgcttccc tcccgcaacg cccgcaacgg
     4021 atatctgttt acgtccgaag ggcgcgtgat cattaagcag gcccggtaca aggaagacga
     4081 gcgtcccgtc gatcccgcct gtcactgtta cacctgccac aacttttccc gcgcctacct
     4141 gcggcacctg ttccaggcgg gcgagatcct ctattcgatg ttggcaaccc tgcacaatgt
     4201 gaagcattat cttgacatca tgcgtctaac gagacagact atactgcttg gaacgttccc
     4261 ggaatttctg agagcggcgc ggtccaaacc ggcggaagaa cagtcagatc cttttgtccg
     4321 ataacaagct ttaggacaag cgggaacgat gcgaatccgt cacgatcatt ttcctttgga
     4381 gcgatgagcg cggtgtacct gacttttctg ctgctgcagg gccaaagtcc ggccggtaat
     4441 cccctcctgg gctttctgcc cattttgctg attttcgcta ttttctattt cctgctgttt
     4501 ttgccgatgc agaggcagaa gaagaaccag cagcgtttgc tgaaagaatt acagaacggg
     4561 aatgtcgtgg tgaccaccgg cggaatcgtt ggaaccatcg ttgcaatgaa tgaagacgat
     4621 acgctagtat tgcgggtaaa gcccgacaac gtaaagattc aggtcgcccg aagtgcagta
     4681 tccggattgc ttcccgaaca ggtcaagaag tagcacgacg cccgatctgt aaggtcatcg
     4741 aatgataaag aatttgaagg tcaaggccgt gatcatcctc gcggtgatac tgctctgtat
     4801 ctacggcatc ataggatttc ccacatccaa ggccaagctt gccgaaaacc tgagcgagaa
     4861 tatccgtctg gggctcgatc taaagggcgg aagccacctc gtgctccagg tgcaggtgca
     4921 ggacgcgttc aaagtggaag cggatcaggc catcgagcgt ttgaaagaag cgctgggccg
     4981 ggctggcgtg cagtatgcat cgctcgaccg cagcgaccct acaagtatcc aggaagcgga
     5041 tcagattcag atcaatatca agggtgtcca gacggtgcag accggtacgc tccgaagcac
     5101 cgtagccgag tcgctgccgt cgtggaacct cgctcccgtc tcgtccaccg actaccggct
     5161 gacgttgaag cagaccgagg ctttgaagct gcggcaggac acggtcaccc aaagcatcca
     5221 cacgatcgag cgtagaatca atggcctggg tcttgccgaa tccgccgtgc agcagcgggg
     5281 acgcgccgat gccgagggtg aaattctggt tcagttgcca ggggtggacg atcccgcccg
     5341 catccggcaa atccttcaga cgacagccat gttggagctt tacgaggtga aggatggccc
     5401 gttcggaacg cagcaggacg cccttgccaa acacggtgga gtgctgccgc tgaatacgaa
     5461 gctggtgaga gccacacccc ggggcgagag cggcgacggc tggtatctgc tcacccggac
     5521 gccggtcatc accggccgag atctccgcga ctcccgtgcg gcccagggtg aattcggccg
     5581 ctgggagacc accttcgtcc tgacccagga cgccgcccgc cgattcgggc ggttcacaga
     5641 acagaatatt ggaaaccggc tggcgatcgt attggacaac caggttctga gcgcccccac
     5701 gatccagaat cggattgaag acaacggacg catcaccggt gccgccagcc agcaggatgc
     5761 cgccgatctt gcgctgaacc tgcgcgccgg atcgctcccc gcgggcattg tgccccttca
     5821 ggaacagacc gtgggaccgt cgctcggagc cgattcgatc cgccagggta tcatcgccgg
     5881 cctggtcggc ctctttgtca tcatcgccat catgcttctc tactatcgcg ggagcggggt
     5941 caatgcaact ctggcgttga tcctgaacgc cgtgatgctg atcgcggcca tggcctggct
     6001 gggtgccacc atgacgctgc ctggcatcgc cggtgtcatc ctcaccattg gtatggccgt
     6061 cgatagcaac attttgattt tcgaacgcat ccgcgaggag ttgcgagccg gcaaggcggt
     6121 cgtggcggcc atggaagccg gtttcagccg ggcgtttctg acgatcatcg atacgcacgt
     6181 aacaaccgtt atctcttgcg cttttctttt tatctttgga tcgggaccgg tta
//
LOCUS       DASTMK010000523        13658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_635350, whole genome shotgun sequence.
ACCESSION   DASTMK010000523 DASTMK010000000
VERSION     DASTMK010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13658
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635350"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..629)
                     /locus_tag="VFQ79_21630"
     CDS             complement(120..629)
                     /locus_tag="VFQ79_21630"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142337.1"
                     /translation="MEGRSFTVVETSARAKVAVVSQATARKLWLTGSPRGQAFAIDPR
                     SAEGATAAGVYQWSVSCRMSVRQARELGADVIKADPTDDSADYHEVIQAARCPVLVRG
                     GGREPVETVFRKAHALLQQGARGLVYGRNVYQHAHPAVIVKAFLGMLHDGLDGEQAWA
                     LYREHEQAS"
     gene            837..2762
                     /locus_tag="VFQ79_21635"
     CDS             837..2762
                     /locus_tag="VFQ79_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142338.1"
                     /translation="MPNGQPGAGEPPREIPWQLAASTRTLDLLTQPPVGSSISILTHV
                     PRLETLDQDFPNDRLVYLKVSVSLSPDRRVIEQAMEEDVARLLLSGGPPIWVSVVDVR
                     LSPIPDQEQAITPHFVSASPLRRTAGETGVIGTSNYEGASEGIATGKSGAEIHEAFQS
                     AVTEETSGGGGYVALLPFIAGGGGSTTTTDISGRRDVYQFQDTLTRQAAEERRDLLSH
                     MTRVHNALTLLSGLYVGSPYLKFVLRPRPLRALSIDPNDPNLWQHELLRRRSSGLEGI
                     QDFYSVAVIPRNLEGFCVSVRLRNVYVLDAPPPPLIDDWISFFYDPPNFYEMLEYLTT
                     RYPRGTPADELDVDLIGTGPRSAWLSPSVAGYGALGPNADEGWVVVAIKGIDPGGGNT
                     VLDRYVPYKTLETVWLETRRAAHQEAVSQSPLERGVVTYVPHEVGVCFDFQGDGTLRR
                     RSPTIRMGDTPMIPSYEEDFPGGPGTIGRAGGGLPRQEARRNWARWANLEDELVARAS
                     NTGDWGDTKLDLQHPRMFDAVLRALSQLPAGRAANVPLSALRQRYGLTNEQIKALKDA
                     GIRDLRGLAQAVRLGNRLTDALAFQEELSRQQAQQRKASDALKAPVPASPEVLNAVRA
                     ALASALARKDSNGHRKK"
     gene            2773..4377
                     /locus_tag="VFQ79_21640"
     CDS             2773..4377
                     /locus_tag="VFQ79_21640"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU0142339.1"
                     /translation="MNGVPSNRALLGPIVGHTDHQGSIIWVRVKSNPHDFALRLHGRS
                     TLIPFVSTEAGPVEFGTAVATVGGLRPEHTYTYQVMHRGRAVAGARGSFRTMPPPGSA
                     ASLSFITLSCSDAKKLGAWQLLQEYIETFEPHFVLMIGDQIYMDGEAETSVWDHHLDS
                     PPQVRRRAMVEMYQEHWDREPVKTILANTPTYMVWDDHDIRDGWGSWAGESPTLQAKY
                     PRGAPITEQYSRYFDDARDIYWHFQMTHNPPPLFGPSTPPVTGERRAMPFAFRCGRLA
                     VLLVDSRGERDVFRPDYPILGDRQWRASLSWLRNLPPSVDALACVTPVPIVAMDPGGL
                     TQSLFQHRTDDEVLYREGRAQDLLVLQKENGSFLDIPFNLAGRALQANIGAYRARSID
                     DLRDQWSQRFSQPEQEAILRTIAQARFANRLPGRPRAAFFLGGDLHIGGRFEIRYDEP
                     EWVTECMIASGISREENITAAQGIVVNESFEVAPGINAELKQYLRVVNFGVTQVQFFG
                     ERALVEHRVLHPGAEVHARVLATSVF"
     gene            4403..4558
                     /locus_tag="VFQ79_21645"
     CDS             4403..4558
                     /locus_tag="VFQ79_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142340.1"
                     /translation="MPAAETVLRTRAKQLPIGLQITGPAGREDLVLTLARAYQSETNW
                     HTERPAL"
     gene            complement(4565..5422)
                     /locus_tag="VFQ79_21650"
     CDS             complement(4565..5422)
                     /locus_tag="VFQ79_21650"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEU0142341.1"
                     /translation="MHNFSNARLNRLFHPSGNCLNVAIDHGVFNERSFLTGLENMGRV
                     VAELVDAGPDALQMNYGQADLLQSIPGKSKPALVMRLDAGNPYSAHLPTVPFDILVRA
                     DDPVLPAVQMDAACVVCNLLLFPGDPDLHRQTVANVGKARAECDRYGMPLMVEPLVMK
                     FDEARGAFQSDGNKNLIVPLVRQARELGADVIKADPTDDPADYHEVIQAARCPLLVRG
                     GGREPVETVFRKAHALLRQGAHGLVYGRNIYQHAHPSVIVKAFLGMLHDGLDGEQAWA
                     LYREHEQAS"
     gene            complement(5790..8012)
                     /locus_tag="VFQ79_21655"
     CDS             complement(5790..8012)
                     /locus_tag="VFQ79_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142342.1"
                     /translation="MPMFLAGIGWVAEELTGNRFRWTIDPEQVVEGRFRGLPDQVVLQ
                     RAPLAVSFGDTLFADSPVFPVPLSWWQNLGNIQSSGIFPARVTLPQQVQAVRFTYAGP
                     DAFFRAWESPGFGRTLVAERSVSNGEVVLLEASLIQELEFFTGAVQLQNVSILNLFAD
                     HAAGLNFESVARVSPRATMGMPLATAYQRYGGTATIDEENWAEFQEVLIPQAVASAPD
                     SAFPLWSTPWQQVQLLLGARWEYAVVYGFGFLDGPDAGPGTSADQIGENLLQEMGSTG
                     HVYRAFALFADGTAETSNLIVIPAFPAMPLPVPTNLGYANPEVRLYGEQTYTVTSSLQ
                     WHATSRRVIGVDIEEEVGPSPILDSATEVIAFTYRSSDPSAVFPAGTMARRFDVPFYD
                     IPLRFRARSVDGWDRVSAFSPWSLPVVPAFIHNPQAPPLQDARFDGAQIALRAQTEHL
                     SFTEWLPDHAVMHTPGSRLQVLRRVSPPENATVTLAEPSVYQDALNQQARLYSVPVPP
                     IPDPGRFLGGYLTSGRFRAEILRHENGRYIFEPVSGSTYGTPMFEGGQATLQQSPNHP
                     DLFTPVADFPVADLPHVLEFPDALAPAGEIVRIEEYGARVRFGAIRGAIGNVVQALVI
                     PGIPEAPPPFSVEFLGVDFYDRTLVRAAFLQEITTGKYSLFWAEGNHDEESFDKAAVA
                     GDYGAQRPQDDHHIYELFSLPVPRNQEAPVTFGAQRETDGAYKSGFTLVHTVVPVYGP
                     "
     gene            complement(8017..11988)
                     /locus_tag="VFQ79_21660"
     CDS             complement(8017..11988)
                     /locus_tag="VFQ79_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142343.1"
                     /translation="MALGFEEKIEHRIYSAAADDSGDPTMTDGSGGWIEEKVLSYLAG
                     LQPGKRLSASPLVKVMEVTTEAVTFLIRGRMELLKGDVAPFVRIEVTVLKTQLGIESV
                     RTFIGELHMVQSQLTPISDVRMGLGYDGLVFMGRGSYRLVPIGFGLDIFLGGVSDRGI
                     MLGINVYLPAPIPLGPTAVGLNGLGGDYAHNFKPRLEAGLIAEPPPVDLETGVTPDPE
                     APLPIENPTATDYARWARNPDDALDRWVAAANDEKTTGVAIRAMFCDMMSAGNILLLD
                     PLGFAVLLPGPVIILGGKGKLLRSNSTVLEAYLVLDGVSRTWSLAGGVKLKIPESGQI
                     VDGAGSVEAFFSLPNPRDWFLNFGTRREPNKAKIIQLMEAKAFLQIDHYRITFGVDVT
                     WSIEGPPGLKKILLFFLKGGLGIYALIGWNPGQFAAEVSLHGEAGVKIFSFITISFSM
                     SVVFFGHVPKPKILRGTLTFKLKLPFPLKSPEIKITFPDIVEDEPPPIEPALQTETRQ
                     EVHVGALHGLTGRQWDLDDSEQSELNQPWPDSELVVHFSRQTTDATGKVLGEPVGAEN
                     NGGYEVRHMLNTLEIFDLVNDVEIGNVESVWAEAPDGSTGQLHVLAQDPYSWLFWSQE
                     ITDSLSFPPPQITLQRFGIGADETLESERRFGDLRVTPQGEAALANTFSFVLPTRTIS
                     ATSLRIVFGGGPVDRILLYVVYPLQAITTFLGGGISATAGGQILALNQIQSFQLSQGV
                     YLLVLEATPPAGAAVEEIIVEAAPGPGGPITGFHLYAVRFRKALQPVGNCQERVILTP
                     GRYRLRVAGETSAESLHDLSNPEPIPWELTREFQIVYPPSLRPYLKYTTVGDSRLFRN
                     ENADWNPTLYGIGFPAYRSYLPVLRFLTPYISRIFTTLTVQIRYVEHDGAVAEFAGEP
                     IENPDGESSTLAPSAEFREWLGCEVQRDQELVLEPLPFSGSAEVRVLFNPPGQPAAIL
                     DSWECYVSQFQHFTEHLTLTHPRITTYYDANGPHSLELCFTPGFAGSRRLPIGGNHID
                     TDPGSFTALPVPRVNPGPLLPGEYPSITTNPFPEELEAPPLPWRLPGNLVSHLTGSAP
                     LGISYARFAHDTGARFSEDPANPLFGINNTVSETRIDAIEDSAGRLFALWLRTPEPVD
                     WRRVSLSLRIRHLQGGSPCPADYAYRRPLDLNVEALPSPDGSSAFLAGRLAGIRTRLP
                     RGEYALTISFDASEAGLPKLRPSAAVGSPETLELKFLNLSGPDWPLPTEDVMVPAGVL
                     AVLVEQAGVPDSILAGLPQDQTDIAPRAPGERLRPRVAPSAGARFRRRQRKEILRAES
                     RRE"
     gene            12029..13555
                     /locus_tag="VFQ79_21665"
     CDS             12029..13555
                     /locus_tag="VFQ79_21665"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0142344.1"
                     /translation="MSGPSKVLTIFFNDLYFWVNRDMAGKRQYQFGPYLFEATSGLLT
                     NGSDRVALRPKIANLLRVLVESAPEVVSKDDLMARVWPDTNVVESGLARNINELRKSL
                     SKEPQAIETVPKRGYRFTIPVECPGPLPEVMATPATAASAPGHSSWARRAAIFAFGTM
                     GAVASASLLIRITRSDDTSAPRTPEPYSAAGIEAEVKKEGAAGYYNWGKWEVQGMRDA
                     LVHFRRAASLDARLWYGYVGIADSYLGLLLLANRMDPEWAQWARHAADRAVQLGPNIA
                     IAHCALGCVRLILDWDLKGAEASFEDALRCNPFNYVAYQRRGLLRTLQRRFDEARTDL
                     RKATELKPEFHDAQVFWAWSEFCAGNFDGAIDVLKDLPPSGGKRKQALRILAATHGMK
                     RQFESARKTLALASLSPGDDLAVRAWLDAREGNVAAAKSGLDRLCAVYARDNLSYCDT
                     AIVNSELGLVDSAFASLERGLRLRHWRVLMLSVDPRLESLHKDPRWYAFCSRIRAREP
                     "
BASE COUNT         2722 a         4019 c         4373 g         2544 t
ORIGIN      
        1 tgccgatcgg cttgcagatt acaggccctg ccggacgcga agacctggtg ctggcgctgg
       61 cacacgccta tcaatcggaa acgaagtggc acaccgagcg gccagcgttg taaacgcctt
      121 caactcgcct gctcatgttc ccggtacaga gcccacgcct gctcgccgtc gagaccgtcg
      181 tgcagcatac ccagaaacgc cttcacgata acagccggat gggcgtgctg atagacattg
      241 cggccgtaga ccaggccgcg cgcgccctgc tgcaaaaggg catgggcctt tcgaaacacc
      301 gtctctaccg gttcccggcc accgccgcga accagcaccg ggcatcgggc ggcctggatg
      361 acttcgtggt aatcggcgga gtcgtcggtg gggtccgcct tgatgacatc cgcgcccagc
      421 tcgcgcgcct gccgcaccga cattcggcac gacaccgacc actgatagac acctgcggcc
      481 gtcgcgccct cggcgctccg cggatcaatc gcgaatgctt ggccgcgcgg cgaccccgtg
      541 agccagagct ttcgggccgt ggcctgactg acgacggcta ctttcgctcg cgcagacgtc
      601 tccacgactg tgaagctacg gccttccacc ggcaaggcca gcgtctcgaa gttagcgatg
      661 gtcgacgtag ttatatgaca gaagcgtggt gcgtccctca acggtgacgg gaagccgacc
      721 cagccgcccg tacaagggca catgcgccac ggctgtcaca gcgccgaagc cggggcgctt
      781 cctatcattc ctcggccgtt gacagactag ccgggttgac ccatactctt ttaatcatgc
      841 ccaatggaca gcccggagca ggcgaaccgc cgcgcgaaat tccgtggcaa ctggcagcaa
      901 gcacacgcac gctggacttg ctgacgcagc caccggtcgg ctcatcgatc tctatattga
      961 cccatgtccc ccggcttgag acgttggacc aggactttcc gaacgatcgc ctggtttacc
     1021 tgaaggtttc ggtttcgctt tcgcccgacc ggcgggttat agagcaggcg atggaggagg
     1081 acgtggcgcg gctgctgctg agcggcgggc caccaatttg ggtttccgtg gttgacgtgc
     1141 gcctcagccc gataccggac caggaacaag cgatcacgcc gcattttgtg tcggcttcgc
     1201 cgctgcgacg aacggccggg gaaacgggag tgatagggac gtcgaattac gagggcgctt
     1261 cggaagggat cgccaccggc aagagcggcg cggaaataca cgaagcattt caatcggcgg
     1321 taaccgagga aacatcgggc ggcggcggct atgtcgcgct gctgccattt atcgccgggg
     1381 gcggcggctc aacaacgaca accgacattt ccggccggcg cgacgtttac caattccagg
     1441 acacgttgac gcgccaggcg gcggaggagc ggcgcgactt actgagccat atgacgagag
     1501 tccacaatgc actgacgtta ctaagtgggc tctatgtggg ttctccgtat ctgaagtttg
     1561 tgctgcgccc gcgtccgctg cgggcgctga gcatagaccc caatgatccg aacctctggc
     1621 agcacgaact gttgcggcgc cggtcctcag ggttagaggg catccaggat ttctacagcg
     1681 ttgcggtgat tccgcgcaat ctagaagggt tctgcgtgtc ggtgcggctg cgcaacgtgt
     1741 atgtgctgga tgcaccgccg cctccactta tcgacgactg gatttcgttc ttctacgatc
     1801 cgccaaattt ctatgaaatg ctcgaatatt tgacaacgcg gtatccacgc ggcacaccgg
     1861 ccgatgagtt agatgtcgat cttatcggca cggggccgcg cagcgcgtgg ctatcacctt
     1921 ccgttgcagg ttatggcgcc ttgggcccga acgcagacga gggatgggtg gtagtagcga
     1981 taaagggcat cgatcctggc ggcgggaaca cggtgttaga tcgctatgtg ccatataaga
     2041 cgctggaaac cgtatggctg gagaccagac gcgcggcgca ccaggaggcg gtttcgcaat
     2101 caccgctgga gcgcggcgtg gtgacctatg tgccgcacga agttggagta tgcttcgatt
     2161 ttcagggcga tgggacattg aggcggcggt cgccgacgat tcgaatgggg gatacgccaa
     2221 tgatcccgag ttatgaggag gatttccctg gcggcccggg cacgatagga agggctggcg
     2281 ggggactgcc caggcaggag gcgaggcgga actgggcgcg ctgggcgaac ctggaagacg
     2341 aactggtggc acgagcgtcg aacacggggg attgggggga cacgaagctc gaccttcaac
     2401 acccgcgaat gttcgacgct gtgttgcgcg cgcttagcca gttgccggcc ggtcgcgccg
     2461 cgaatgtccc tctgagcgct ttgaggcagc gctacggatt gactaatgag cagatcaaag
     2521 cgctgaagga cgccggcata cgcgatttgc ggggactcgc tcaggctgtg cgattgggga
     2581 accggttgac cgatgcgctg gcgttccagg aggaactgag cagacagcag gcacagcagc
     2641 gaaaggcgtc ggatgcgctg aaggcgcctg ttccagcatc gcccgaggtg ctgaatgccg
     2701 taagggccgc gctggcatca gccctggcgc gtaaggacag caacggccac cgcaaaaagt
     2761 aagttgggcg agatgaacgg cgttccgtcg aaccgcgcct tactcgggcc gattgtgggc
     2821 cacacggacc accaggggag catcatctgg gtccgtgtga agagcaatcc gcacgatttt
     2881 gcattgcgac tgcacgggcg atcgacactg attccgtttg tgtccaccga ggccggaccg
     2941 gtggagtttg ggaccgcggt ggctacggtc ggaggcctgc gtcccgagca cacctacacc
     3001 taccaggtga tgcatcgcgg gcgggcggta gcgggggcgc gaggctcgtt ccgcacaatg
     3061 ccgccgccgg ggtcggctgc gagtctttcg ttcatcacgc tctcctgcag cgatgcgaag
     3121 aagttggggg catggcagct tttgcaggaa tatatcgaga cgttcgagcc ccacttcgtg
     3181 ctgatgatcg gcgatcagat ttacatggat ggggaagcag agacaagcgt gtgggaccac
     3241 cacctggatt cgccgccgca ggtgcgcagg cgggcaatgg tggagatgta ccaggagcat
     3301 tgggaccgtg agccggtgaa gacgattctg gcaaacacgc ccacttacat ggtttgggac
     3361 gaccacgaca tacgcgacgg atggggatcg tgggccggcg agagtccgac gttacaggcg
     3421 aagtatccgc ggggggcgcc aattacagag cagtattcgc ggtatttcga cgatgccagg
     3481 gacatttact ggcactttca gatgacgcac aatccgcctc ccctttttgg tccaagtact
     3541 ccaccggtga cgggcgagag gcgcgccatg ccctttgcgt tccggtgtgg gaggctggct
     3601 gtgttgctgg tggacagccg cggggagcgc gatgtgttcc ggccggatta cccgattctg
     3661 ggcgaccggc agtggcgggc gtcgctgagc tggctacgca atcttccgcc gtccgtagat
     3721 gcgctggcct gcgtgactcc ggttccgatt gtagcaatgg acccgggcgg gctgacgcaa
     3781 tcgctgtttc agcatcgcac cgacgatgag gtgctgtatc gcgaagggcg ggcgcaggac
     3841 ctgctggtgc ttcagaagga gaacggcagc tttctggata ttccattcaa cttggccggg
     3901 agggcgttac aggcgaacat cggcgcgtat cgcgcgagga gcatcgacga cttgcgcgac
     3961 caatggtcgc agcgcttcag ccagccggag caggaggcga ttctgcggac gatcgcgcag
     4021 gcgcggttcg ccaatcgtct gccggggcgt ccgcgcgcgg cattcttcct tgggggcgat
     4081 ctgcatatcg gcgggcggtt cgagattcgc tatgacgaac ccgaatgggt gactgaatgc
     4141 atgatcgcgt cgggcatcag ccgcgaggag aacatcacgg cggcacaggg cattgtggtg
     4201 aacgagtcct ttgaagtagc gccggggatc aatgcggagt tgaagcagta ccttcgcgtt
     4261 gtgaatttcg gggtgacgca ggtgcagttt tttggggagc gggcgctggt ggagcaccgg
     4321 gtactgcatc cgggcgcgga ggtgcacgcg agagtgctgg cgacatcggt gttctgaagc
     4381 tggcggtcag aagccgatgg agatgccggc agcggaaacg gttcttcgca cgcgcgcgaa
     4441 gcaactgccg atcggcctgc agattacagg ccctgccggg cgtgaagacc tggtgctgac
     4501 cctggcacgc gcctatcaat cggagacgaa ctggcacacc gagcgaccgg cgctgtaaac
     4561 gtcttcaact cgcctgctca tgttcccgat acaaagccca cgcctgctcg ccgtcgaggc
     4621 cgtcgtgcag catacccagg aacgccttca cgatgacaga cggatgggcg tgctgataga
     4681 tattgcggcc gtataccaag ccgtgggcgc cctgccgcaa aagggcatgg gcctttcgaa
     4741 acaccgtctc caccggctcc cggccaccgc cgcgaaccag caacgggcat cgggcggcct
     4801 ggatgacttc gtggtaatcg gcggggtcgt cggtagggtc cgccttgatg acatccgcgc
     4861 ccagttcgcg cgcctgccgc accagcggaa cgatcaggtt cttgttgcca tcggactgaa
     4921 acgcgccgcg agcttcgtcg aatttcatga ccagcggttc caccattaaa ggcatgccgt
     4981 aacggtcgca ttccgcgcgc gctttgccga cgttggctac cgtttggcgg tgtagatccg
     5041 gatcgccggg aaacagcaga aggttgcaaa ccacgcaggc agcgtccatt tgaaccgcgg
     5101 gcaacacggg atcgtccgca cggacgagaa tatcaaacgg cacggtgggc aggtgcgcac
     5161 tgtaagggtt gcccgcatcc aggcgcatga cgagcgcggg tttcgacttg ccggggatcg
     5221 attgcagaag gtctgcctgg ccgtagttca tctgtagggc atcgggtccc gcatccacca
     5281 gttccgctac cacgcggccc atgttctcga gcccggtaag aaagctgcgt tcattgaaca
     5341 cgccgtgatc gatcgcaaca ttgaggcagt tacctgaagg atggaagagc cggtttaaac
     5401 gcgcgttgga gaagttgtgc atcgcctctg cccatcaaga tagcagcatc ggcggggaac
     5461 cggagaattt gcaaacgccg cggcatcagc gtgcgaaacg cctgctgaac catgcgtcag
     5521 taacgggcgt gatacggaca agtggatgtg ggattccaag aaatctcacc ttcacggtcc
     5581 ctaccttcag taagagccag tttcggccaa ttcaagcatt tactactcaa cacgtgcgag
     5641 aattagcttc ctcgcgcgaa tgtctattgt gacgacaaag tgcgacaaga agtcgcttcc
     5701 aagcgtcagt gcaaccggca gatcaaaccc atttctccag cggatgggag attcgcgcac
     5761 cgtgcgaagc tagccggcac atggcctcac tacggcccat ataccggcac caccgtgtgc
     5821 accagtgtga acccggactt gtaggcgcca tccgtttccc tctgcgcgcc aaaggtcact
     5881 ggagcttcct ggtttcgcgg cacgggaagc gagaacagtt catagatgtg atggtcgtct
     5941 tgaggtcgct gtgcgccata atcgcctgct actgcggcct tgtcgaagga ttcctcatca
     6001 tggtttccct ccgcccagaa gaggctgtac tttcccgttg tgatctcctg caggaatgcg
     6061 gcccgcacga gggtccgatc gtagaagtcg actcccagga actctaccga aaagggcggc
     6121 ggggcttcgg ggatgcccgg gatcacgagc gcctggacca cgttgccaat cgcaccgcgg
     6181 atagcgccga accggacccg cgcgccatac tcttcgatcc ggacgatctc gccggccggc
     6241 gccagggcgt cgggaaattc gagaacatgg ggaaggtccg cgaccggaaa gtcagcaact
     6301 ggcgtgaaaa ggtcgggatg attcggggac tgctgcagcg tggcttgccc accctcgaac
     6361 atcggcgtcc cataggtgct gccggacacc ggctcaaaga tgtagcggcc gttttcgtgg
     6421 cgcagaatct cggcccggaa acggcctgaa gtgagatacc cgccaaggaa acggccagga
     6481 tcagggattg gcggtacggg tacggaatag aggcgcgcct gttggttgag ggcgtcctga
     6541 tagacggacg gctccgcgag agtcaccgta gcgttctccg gagggctgac gcggcgaagg
     6601 acctgcagcc ggctgccggg tgtatgcatg accgcgtgat ccggcagcca ttcggtgaaa
     6661 ctcaaatgct ctgtctgcgc ccgcagcgcg atctgagcgc cgtcaaaacg agcgtcctgc
     6721 agcggcggcg cctgcggatt gtgaatgaag gccggtacga ccggcagcga ccagggactg
     6781 aaggcgctga cccgatccca tccgtcgacg ctgcgggcgc gaaagcgcag ggggatatcg
     6841 tagaacggca catcaaagcg cctcgccatc gttcccgccg ggaaaaccgc agaggggtca
     6901 gaactgcgat aggtgaaggc gattacctcc gtggcgctgt cgagaatcgg gctcggcccg
     6961 acctcttcct cgatgtcgac gccgatgaca cggcggctgg tggcgtgcca ctgcagggaa
     7021 ctggtcacag tgtaggtctg ttcgccgtac agcctgacct caggattcgc atagccgaga
     7081 tttgtgggga ccggaagggg catcgccgga aaggcgggga tcacgatcag attgctcgtc
     7141 tcggctgtgc catccgcaaa tagcgcgaag gcacgataga catggcccgt agatcccatc
     7201 tcctgaagca ggttttcgcc gatttgatcc gcgctggttc ctgggccggc atcagggccg
     7261 tccagaaatc cgaagccgta gaccactgca tactcccagc gcgccccgag cagcaattgc
     7321 acttgctgcc agggcgttga ccacagtggg aatgcgctgt cgggagcgct ggcgaccgcc
     7381 tgaggaatca ggacctcctg gaactcggcc cagttctctt cgtcgatcgt tgcagtgccg
     7441 ccgtagcgct gatacgcagt cgcgagcggc atacccatgg ttgctcgcgg cgacacccgg
     7501 gcgacgcttt cgaaattcag acccgcggca tggtcggcga ataagttgag tatcgagacg
     7561 ttctgaagct ggactgctcc cgtgaagaat tccagttcct gaatcaggct ggcttcgagt
     7621 agaactactt caccgttgct caccgatcgc tccgcgacca gggtgcggcc gaaaccgggg
     7681 ctctcccacg cgcggaaaaa cgcgtccggc cctgcatagg taaagcgaac agcctgcacc
     7741 tgctgcggca gcgtgaccct ggcaggaaaa ataccgctag actgaatatt ccccaaattc
     7801 tgccaccagg aaagagggac cggaaagaca ggcgagtctg caaagagcgt atcgccgaag
     7861 ctcaccgcca gcggagcgcg ctgcaagacg acctgatcgg gaagtccacg aaagcggccc
     7921 tcgacgactt gttccggatc gatggtccag cggaaacgat tccctgtgag ttcttccgca
     7981 acccaaccaa tacctgcaag gaacatgggc atagacttat tccctccggg actccgcgcg
     8041 cagaatctct ttcctctgcc gccggcggaa gcgagcgccc gccgaggggg caacacgggg
     8101 ccgcaatcgt tctcccggag cgcgcggcgc tatatccgtc tggtcttgcg gcaggccggc
     8161 gagaatcgaa tcgggaactc ccgcctgctc cactaacacc gccagcactc cggcaggaac
     8221 catgacgtct tcggtgggaa gcggccaatc cggcccgctc agattcaaga acttcaactc
     8281 caaggtttcc ggagatccca ccgcggcaga gggccgcagc tttggcaaac ccgcctccga
     8341 tgcatcgaac gagatcgtca gcgcgtattc tcccctggga agccgtgtac ggatgccggc
     8401 aagccggccg gcgaggaacg cggaactccc atcgggggaa gggagcgcct caacattcag
     8461 gtccagtggc cgtcgatacg cgtagtcggc cggacatggg ctgccaccct gaagatgacg
     8521 gattcgcagc gagagtgaga cacggcgcca gtccaccggc tcaggcgtcc ggagccacag
     8581 tgcgaacagg cggccggcac tgtcctcgat ggcatctatc ctggtctcgg agaccgtatt
     8641 gttgatgcca aataacgggt tggccgggtc ttcggagaag cgggcgcccg tgtcatgcgc
     8701 gaaacgcgcg taagagatcc cgagcggtgc actgccggtc aggtgtgaga ccaaattgcc
     8761 aggcaagcgc caggggagtg gcggagcctc aagttcctcc gggaacggat tcgtcgtaat
     8821 ggatggatac tcgcccggca gcaatgggcc gggattcacg cgcgggacag gcaaggccgt
     8881 aaagctccct gggtcggtgt cgatgtgatt gccgccgatc ggcaggcgcc gggagcccgc
     8941 gaatccggga gtgaagcaga gttccaggga atgcggcccg ttggcatcat aataggtcgt
     9001 gattctcgga tgcgtgagtg tcaggtgctc ggtgaagtgt tggaattgtg agacgtagca
     9061 ttcccagcta tccagaatcg ccgccggctg gccgggcggg ttgaacagga ctctcacttc
     9121 cgcggagccg gagaacggaa gcggctccag aaccagttcc tggtcgcgtt gcacctcgca
     9181 gccgagccac tcccggaact ccgcgcttgg tgccaaggtg ctgctttcgc cgtccgggtt
     9241 ctcgattggc tctccggcaa actcggcgac ggcgccatcg tgttccacgt agcggatttg
     9301 cacggtcaga gtcgtgaaga tgcgcgaaat gtacggggtc aggaaccgca gcacgggcag
     9361 atacgaacgg tacgccggga aaccgatgcc gtagagcgtc gggttccagt cggcgttctc
     9421 gttgcgaaag aggcggctgt cgccgaccgt ggtgtacttc agatacggcc gcagagaggg
     9481 tggatagacg atttgaaact cgcgggtgag ttcccagggg atcggctcag ggttgctcag
     9541 atcgtgcagg gattcggccg aagtttctcc ggcgacgcgg agacggtagc gcccgggagt
     9601 aagaatgacg cgctcctggc aattgccaac cggctggagc gccttccgga agcgcactgc
     9661 gtagagatgg aatccggtga tcggcccgcc aggccccgga gccgcttcca cgatgatttc
     9721 ctcaacggca gcgcccgccg gtggcgtcgc ctccagcacg agcagataca cgccttgcga
     9781 tagttggaaa gactgtatct ggttcagggc caggatctgg ccgccggcgg ttgcactgat
     9841 cccgccgcca aggaaggtcg tgatcgcttg aaggggatat accacataca gcaggatccg
     9901 atccacgggg ccgccgccga acacaattcg cagcgatgta gcggaaatag ttcgagtcgg
     9961 aagaacgaaa ctgaaggtgt tggcgagcgc cgcttcgccc tgtggggtta cacgaagatc
    10021 gccgaaacgg cgctcggatt ccaaagtctc gtccgcgccg attccaaagc gctgcagcgt
    10081 gatctgtggc ggcgggaacg acagcgaatc ggttatttcc tgactccaga agagccacga
    10141 atacggatcc tgcgccagca catggagttg tccggtagac ccatcggggg cctcggccca
    10201 gaccgactcg acattcccga tttcgacgtc gtttaccagg tcgaagatct ccagcgtatt
    10261 gagcatgtgc cgcacttcgt agccgccgtt gttctccgcg cctaccggct cgcccaggac
    10321 ctttcccgtg gcatcggtgg tctgacgtga gaaatgcact accaactcgc tgtccggcca
    10381 gggctggttc agttcactct gctcggagtc gtcaaggtcc cactgccggc ccgtcagccc
    10441 atggagcgcc ccgacgtgta cctcctgacg tgtctcggtc tgcagtgctg gctctatcgg
    10501 tggcggctcg tcttccacga tgtccggaaa cgtgatcttg atttccgggg atttgagcgg
    10561 gaacggcagc ttcaacttga aggtcagcgt gccgcgtagg atcttcggct tcggcacgtg
    10621 gccgaagaac accacggaca tggagaagct gatcgtaata aacgaaaaga tcttcactcc
    10681 ggcttcgcca tgcagactta cctcagccgc gaactgcccc ggattccagc cgatcagggc
    10741 gtagatgccc aagccgcctt tgaggaagaa cagcaggatc ttcttgaggc caggcgggcc
    10801 ctcgatggac caggtcacgt caactccgaa cgtgatccta tagtggtcaa tctgcagaaa
    10861 ggccttggcc tccatgagtt ggatgatttt ggccttgttc ggctcgcggc gcgtgccgaa
    10921 attcaggaac cagtctcgcg gattgggcag agagaaaaag gcctccacgg agcctgctcc
    10981 atcgacaatc tgcccgctct cgggaatctt cagtttcacg ccgccggcga gcgaccaggt
    11041 gcggctcact ccgtccagca ccaggtatgc ttccagcacg gtggagtttg accggagcag
    11101 ctttcctttg ccgccgagga tgatcaccgg gcccggcaac agtacggcga aacccagcgg
    11161 atccagcagc agaatatttc cggccgacat catgtcgcaa aacatggcgc ggatagcaac
    11221 accggtcgtc ttttcgtcgt tcgccgccgc aacccaccgg tccagagcgt catcgggatt
    11281 gcgcgcccag cgcgcgtagt cggttgcggt cggattctca atcgggaggg gcgcctcggg
    11341 atcaggcgtc acgccggttt ccaaatcgac cggaggcggc tcggcaatca gccctgcttc
    11401 caggcgcggc ttgaagttgt gcgcgtagtc gccgccgagc ccgttgagtc cgactgcggt
    11461 gggccccagg gggatcggcg ccggaagata cacgttgatg cccagcatga tgccgcggtc
    11521 ggaaacgccg cccagaaaga tgtccaggcc gaagccaatc gggacaagcc ggtaggaacc
    11581 tcgccccatg aataccagcc cgtcatatcc aagccccatc cgcacgtcgc tgatcggcgt
    11641 gagctggctt tgaaccatgt gcagttcacc aatgaaggtg cgtacggact cgatacccag
    11701 ttgcgttttg agaacagtca cttcgatccg gacgaacggc gccacgtcgc ccttgagaag
    11761 ctccatgcgg ccccgaatca ggaaggtcac ggcctcggtc gtgacctcca tgaccttcac
    11821 cagtggagag gcggagagtc ttttgcccgg ctgaaggccc gcgaggtaac tgaggacctt
    11881 ctcctcaatc cagccgccgg agccatccgt cattgtggga tcgccggaat cgtcggcggc
    11941 cgcggagtag atccggtgct ctatcttttc ttcgaatccg agcgccatgg ggtgtggagc
    12001 ctctcatcga ttgtgcgcag aagaccgttt gagcggccct tccaaagttc ttacaatatt
    12061 cttcaacgac ctttacttct gggtgaaccg tgatatggcc gggaaacggc agtatcaatt
    12121 cggcccgtac ctgtttgagg cgacgtccgg actgctaacg aacggcagcg atagggttgc
    12181 cctgagacca aaaatcgcca atttgctacg agttcttgtg gaatcggctc ctgaggttgt
    12241 atcgaaggac gatttgatgg cccgggtgtg gccggatacc aacgttgttg aatccggact
    12301 agcgcgcaac attaacgaac tccgcaagtc gctttccaag gagccccaag cgatcgagac
    12361 cgttccaaag cggggctacc ggttcacaat cccggtagaa tgccctgggc ctcttccaga
    12421 ggtgatggct accccggcca ctgccgcttc tgctccgggc cattcgagct gggcaaggcg
    12481 tgccgcgata ttcgcgttcg gcacaatggg agccgtcgca tcagcgtctc ttctcattcg
    12541 aatcaccaga tccgatgata cgtcggcgcc gcgaacgcct gaaccgtact ctgccgccgg
    12601 tatcgaagcg gaggtaaaga aagaaggagc tgccggctac tacaactggg ggaagtggga
    12661 agtgcaggga atgcgggacg cgttggtgca ctttcgccgc gcggcatcgc tggatgcgcg
    12721 gctctggtat ggatacgtag gtattgccga ttcctatctg gggctgctcc tgctggcaaa
    12781 tcgaatggat ccggaatggg cccagtgggc gcggcatgcc gcggatcgcg cagttcagct
    12841 tggtcccaac atcgccatcg ctcactgcgc tctgggctgt gtacggctga ttctcgattg
    12901 ggacctcaag ggagcagagg cctcctttga agacgcgctc cgctgtaatc ccttcaacta
    12961 cgtggcttac caacgccgcg ggctgctgcg cacgctccag cgcagattcg acgaggcaag
    13021 gactgacttg cgcaaggcta ccgagttgaa gccggagttc catgatgcgc aggtgttttg
    13081 ggcgtggtcc gagttctgcg ccggaaattt cgatggcgcg atcgacgtac tcaaggattt
    13141 gccaccctca ggaggcaaac gaaagcaggc tctgcgaatc ctcgccgcaa cgcatggtat
    13201 gaagcgccag ttcgagtccg cccggaagac tctggcgctc gcgagtttgt ctccgggcga
    13261 cgatctggcc gttcgcgcct ggctggacgc gcgggaaggt aatgttgcag cggccaaatc
    13321 gggcctcgac cgtttatgcg cggtctatgc cagagacaac ctgtcctatt gcgacacggc
    13381 tattgtcaat tcggagctag ggctcgtcga cagcgccttc gcatctctcg aacgaggact
    13441 gcggttacgt cactggcgag ttctgatgct gtccgtcgac cctcgtctgg agtctctgca
    13501 caaggatcca cggtggtacg cattctgctc gcgcattcgg gctcgagaac catagccgat
    13561 catagttgcc ggggagtggc caaaaaccgc ttgctggcgc gcgcggctcg gaaccgagcc
    13621 gtgactgcta gaaatggcgt gttaaatcgc ggaagctt
//
LOCUS       DASTMK010000524        19560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_410962, whole genome shotgun sequence.
ACCESSION   DASTMK010000524 DASTMK010000000
VERSION     DASTMK010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19560
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410962"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFQ79_21670"
     CDS             complement(<1..401)
                     /locus_tag="VFQ79_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEU0142345.1"
                     /translation="MIANADRPVVLSGLRKAAVLMVTLGDQTSSEILKELDEDEVQLI
                     SREIAKISSVPTDQAESVLEEFYEMSMAHDYVLKGGIDYARKVLHNAFGPEQAKRLLD
                     RVVRSLGAEVATFDALQKADPQQLAKFIHSE"
     gene            complement(398..2119)
                     /gene="fliF"
                     /locus_tag="VFQ79_21675"
     CDS             complement(398..2119)
                     /gene="fliF"
                     /locus_tag="VFQ79_21675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEU0142346.1"
                     /translation="MEQLKNLFLSLSMRQRITTGIVALAVIAALFGAAKWNRERDFKL
                     LYSGLSSEDAGAVLAKLKEGNIEYRLEENGSTVLVPSGKVAELRLQMAAAGLPKTGRI
                     GFELFDQTNFGLTEFAEQVNYHRALEGELERSLISLSEVEHARVHITLSKDSLFVESR
                     QPAKASVMLKLRFGSKLSAQNIAGICHLLASAVEGLSPEMVSVLDVRGNLLNRPRPAN
                     PDAAEPSEASLEYRQRIERDLITKINTTLEPLLGPDRFRAGVFVDCDFTSGEQSEETF
                     DPNRSVMVTSQRTEDATGTGGTSGTPGVASNLPRPPSRPVSSAGGLSRRTENITYQSS
                     RTVRHVKLPQGGIKRVSVSLLVDQDVRWEGSGADAKRVLEPPPPEKLKVIRDLVAAAV
                     GFTPDRGDQLIVESLPFETTANWEPPKPDSPPPAQPGTLQLPAWLQKIVGQTNGAVLI
                     GAGAGVVLVLLALMLFLFRRSRNRKRARKQVEMTKEVASGQGQKSIEGDSQNDASMSM
                     EAALAQRAALQQKMNDEALSSLKIQPVSTKKTEVLARHIGDGAKKDPTSMAHVVRTWL
                     NDERTSR"
     gene            complement(2158..2454)
                     /gene="fliE"
                     /locus_tag="VFQ79_21680"
     CDS             complement(2158..2454)
                     /gene="fliE"
                     /locus_tag="VFQ79_21680"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEU0142347.1"
                     /translation="MNPISFNVPGIPNIPSTVVPGAGKQQGGFQVALEGAISQVNELR
                     QVSHQSIDKFLSGEAEEVHQAVLATQQAELAFEMFLQVRNKVVQAYQEVMRMQV"
     gene            complement(2451..2888)
                     /gene="flgC"
                     /locus_tag="VFQ79_21685"
     CDS             complement(2451..2888)
                     /gene="flgC"
                     /locus_tag="VFQ79_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEU0142348.1"
                     /translation="MSLFSLLSVSASGMSAQRARAEVMVENIANSETTRTPEGGPYRR
                     KDVVFATESQTSPFSAVFQSEIGAGVTGVAVSEVVQDTRDLEKRYMPGHPDADANGYV
                     AFPRVNPAEDMVDLMGASRAYQANIAAMSAVKDMIHRSIELMR"
     gene            complement(2885..3205)
                     /locus_tag="VFQ79_21690"
     CDS             complement(2885..3205)
                     /locus_tag="VFQ79_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HEU0142349.1"
                     /translation="MKLERYMDLLSARQKLVASNIANADTPGYKTRDISFQFEFMSQI
                     EGAVPNVVEVPGLVTKNDGNNVNMDRELRLLSENALRFNMASNLLRSQIRTIRSAIQE
                     GRGG"
     gene            complement(3371..3796)
                     /locus_tag="VFQ79_21695"
     CDS             complement(3371..3796)
                     /locus_tag="VFQ79_21695"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEU0142350.1"
                     /translation="MDGLRFACQAGCTACCRQHGYVYLSEADIPKAAGFLGMSPAAFE
                     AKYVYRTRNFIRLRKPRNSQCHFLNGTGCRIHPAKPAQCRLFPFWPELVENVAAWKAT
                     AVYCPGIGTGPLVQIGTALEIADEMRTAYPGMYEPQNKP"
     gene            3852..5741
                     /locus_tag="VFQ79_21700"
     CDS             3852..5741
                     /locus_tag="VFQ79_21700"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0142351.1"
                     /translation="MRRTSRREFCKTIPSGLALAAAANAQQPDGVLLEASAFADHGGW
                     VLDQQSIDRIGSSYLLAHGCGRPVQDAATVVEFPSTGEYHLFVRTRDWVGPWKGANVA
                     PELRAEGSPGVFRLLIEGEPVETVFGAEGADWHWAPGGPVRITKKKVTVALRDLTGFD
                     GRCAAIFFSRTPNPQLPDAPDAVRAWRSRLTGGPAEPAPVGRFDFVVAGGGVAGICAA
                     VAAARHGLKVALLHNRPVLGGNSSSEIRVSPSSVRNIEPYIGLGSILGEFDWNRPVEP
                     PQARVFQDGDDIRLKLVGDERNISLFLNHHVYKVEKEGVVIKAVHTQHVLSGALSRFE
                     GALFADCSGDANLGFLAGAHWRMGREGRPKTGETRAPEKADGQVIGSTLHWYSRTTEK
                     KTLFPDAPWALPFTEEKCQFVTEGGWNWETGFHLDQANQTELIRDHKLRAIFGNWAYQ
                     KNKSERKDWYTDLELAWVGYVLGKRESRRLMGDLVYSQRDLDAGTEYPDGCIACNWGI
                     DIHVPDPKNQKQFAGWAFRAVAEHYDKGRAPMRWLPYRCLYSRNIPNLFMAGRNVSCT
                     HVAFAWFRTQRTTAMMGEAVGLAAALCIDRKSTPRGVYAEHLNKYKEMLKKGAGKVTA
                     GSPKS"
     gene            complement(5753..6520)
                     /locus_tag="VFQ79_21705"
     CDS             complement(5753..6520)
                     /locus_tag="VFQ79_21705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU0142352.1"
                     /translation="MSLSAYIVDDEPLAVDRLRRLLANTRRIEILGSSTDPQAALEFL
                     AHHQVDVLFLDIQMPGITGFELLAKRPSQPVVVFTTAYDQYALKAFEMNSIDYLLKPI
                     DPLQLERALNKIERLCGSLVAAADFRKLFEEVASSLRQRARDYPDRIATRVGERIRFL
                     DLAHVTHFYAEDKLTYAMAGGKPHCVDDTLAGLEQRLDPKRFIRIHRATLLNREWVQY
                     IAPAFAGSYIVRLKDANHTELTVARHRASEVKQRLGL"
     gene            complement(6517..7605)
                     /locus_tag="VFQ79_21710"
     CDS             complement(6517..7605)
                     /locus_tag="VFQ79_21710"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0142353.1"
                     /translation="MLAADLRKPPSIVRVLAICVGGAVVAPFVVVAFGSDWRHTLAGR
                     VFAYTIIHSTCIGLLANLLLPPVERRIGRHGVKLASAYVVVLLVAALAGAVVGSALLW
                     VAGLLPPRPFWQEYWSTARTACLLVLVFGMSAFFYESLVSRLRETRARLQEKELEEER
                     ARKLAAEARLSSLESRIHPHFLFNTLNSISSLIPVAPARAEEIVGQLATLLRSSLDAA
                     PRALIPLEEEMQIVKAYLDIEKARFGERLRYRFDIPQAAEFLLPPLSIQSLVENAVKH
                     GIAPKPEGGEIEVIAQKSANAIRMEVRNSGPCFSLANVDPGHGIDNLVSRLDVLFGPR
                     ASLQVGSRDGWCVVTLEIPAQREGVAAG"
     gene            complement(7612..8433)
                     /locus_tag="VFQ79_21715"
     CDS             complement(7612..8433)
                     /locus_tag="VFQ79_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142354.1"
                     /translation="MKTIAILSLLVLVASSAQIQPRGKWKWTLSASPVAKGAGPQTYR
                     FTCDYYYLDTKGNPGRRERVSALYLRDLPEDRVRWSGVEIAESSGSSGNFGPARKCDF
                     MEGFSYARASLKDMLAPGFFRGFPPTAMFERNLVWDTHMIENFGQDYFPDLKLNIPFH
                     VPQSGALSLAGAGTVTLKDLQLIWTGISQRNGQECAVIDYRAFLNTLDMKMPEISIVA
                     GSNFWGQIWVSLGTKRIEYGTLNEGVVGEMSVQDQKKPWVVNIFRMGVFEPVNNK"
     gene            8687..10024
                     /locus_tag="VFQ79_21720"
     CDS             8687..10024
                     /locus_tag="VFQ79_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEU0142355.1"
                     /translation="MEGIPQIVTSLPGPRASEIIERDRELISPSYTREYPLVAERGEG
                     AIVYDVDGNRFLDFNAGIAVAATGPCHPEVVEAIQRQAARLIHMSGTDFYYVGMVQLA
                     EQLARLAPGGRAWRVYFGNSGTEAIEACMKLARHHSGRDKFIAFFGGFHGRTLGSLSL
                     TASKAVQRAGFGPLVPGVHHVPYPDCYRCPYGKRPDTCAVDCVRHIEEEVFRTTLPPQ
                     EVAAIVVEPVQGEGGYVAPPKNFFDRLAALAKTYGILLVFDEVQCGMGRTGKMWAAEH
                     FDASPDIFAAAKGIASGMPLSATMARASIMTWPPGAHASTFGGNPVSIAASLATIELL
                     QRELIANAARLGEHILSRLRAWPRKFPFVGDVRGLGLMIGIEFVRDQGSKERAPEYRN
                     RILQLAFERGLLVLGAGRNSIRLCPPLVITRDQADFAVDTLEDCLLVLKRESS"
     gene            10011..10544
                     /locus_tag="VFQ79_21725"
     CDS             10011..10544
                     /locus_tag="VFQ79_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0142356.1"
                     /translation="MKVLDVGCGLKKLPGAIGIDRNPSTAADVVCDLDSFPYPFRDAA
                     FDAIRAVHVIEHVSDVVRTMEEFHRLLHPGGRVEIVTPHYTDFSSFCDPTHRWHLNSF
                     SFRYFGEDDAGFGYYSAARFREISVRVKLLRLWRALGFELLVNRFPRFRRFWEHYLCF
                     VVRGKVIEFEFEKPAGA"
     gene            10560..10982
                     /locus_tag="VFQ79_21730"
     CDS             10560..10982
                     /locus_tag="VFQ79_21730"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU0142357.1"
                     /translation="MIQHLLGFADSMRHRARRRNSDPGQEMGRRGEDLAHRFLRKAGY
                     TIVARNYRTRSGSGELDVIAWDGDTLVFVEVKSRGSEEFGAPDRAIGEEKRTSIARAA
                     RDYARRADVAWNRVRFDVVNVIVDARPEITHIKDAFYS"
     gene            11067..12068
                     /gene="galT"
                     /locus_tag="VFQ79_21735"
     CDS             11067..12068
                     /gene="galT"
                     /locus_tag="VFQ79_21735"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU0142358.1"
                     /translation="MPELRKDPITGRWVIIATDRAKRPSDFSREQVIPKGGGFCPFCP
                     GNELKTPPEILAYRENGGPNEKGWTLRVVPNKFPALRVEGDLNRQGQGLYDKMTGIGA
                     HEVIIETPEHNLTLADLPEKQVADVFWAFRDRILDLKNDTRLRYVLVFKNHGEAAGAS
                     LEHTHSQLIALPVVPKRVQEELDGARRYFDFKERCVYCDIIRQELEMGTRVVLETDHF
                     IAVEPFAPRFPFETWILPRIHHSHFEASTAAAIQNLAWITRQTIRKVDKVLERPPYNM
                     MLHSAPVQEGSVSYYHWHIELIPKLTKVAGFEWGTGFYINPTPPEEAAKFLREVGLV"
     gene            complement(12010..12393)
                     /locus_tag="VFQ79_21740"
     CDS             complement(12010..12393)
                     /locus_tag="VFQ79_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142359.1"
                     /translation="MSRFMENATRIFEAVESAADAGSTLSNLTILINSGGGIRLVADC
                     DWPLDSLQAERGAGMVYRISQQEQTVRVEGRAGSRTCLFETETTKGVARRLLAGTPLY
                     FVEAPQSTPALLPAGTSQLPPAVWG"
     gene            12519..14948
                     /locus_tag="VFQ79_21745"
     CDS             12519..14948
                     /locus_tag="VFQ79_21745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142360.1"
                     /translation="MKKPLPTRGVVVSLILLFSLSVFAQEATEPPADLQEGSAGSPPT
                     VYESVTVTARRVEEEAQEVPIPVSVVSGALIDRAQAFNVNRLKELIPTVQFYSSNPRN
                     SAINIRGLGSPFGLTNDGIEPGVGFYVDGVFYARPAAATLDFLDLERVEVLRGPQGTL
                     FGKNTTAGAINITTRRPSFTPEANFELNYGNYGFVQAKTSVSGPLSRKIAARLSFSGS
                     QRDGMLYNVRTEDDVNDLNNLGVRGQVLFTPAPSLLVLFAIDYTRQRPEGYAQVVAGV
                     APTLRAANRQYPGIAADFDYRPPSFNAFDRLIDTDIPWRSNQDMGGASVTVDWDRGPG
                     VLTSITAWRFWDWDPSNDRDFIGLPVTTISAAPSKQRQWTQVVRYAGRITSGMNFLVG
                     AFLFHQKLNTDPFHKQEQGAAAARFLLAPSALAATPGLLDGYGQNVNFDFSNTSSAVF
                     GQIDYGLTNRLRLIPGLRVNYDQKDVVYDQQLYGGLKTTDPALIALQRSILAPLSYKA
                     DAGDVNVSGQLTLAYKAGESANAYASYSTGFKSIGLNLGGVPTDAAGQPITSAATVRP
                     ERVRHLELGLKTQPLRNVTANITLFNTGIKDFQTQVLNAQVGVLRGYLANAEKVRVRG
                     IELDANAMVTPELSAYISAAYSDGRYLTFRDAPPPLEETGGPQVKDISGSVLPGISKW
                     AFSAGGEYAKRGSLLSTTGTYFARVDTSYRSSFSSNPSASRYLIADSYGLVNPRAGFR
                     SVDGWTISVWARNLLNTDYFELLAAAPGNSGLYTGLPGDRRTFGVSFGWTFYRGSRPV
                     PTPTRAQSPKD"
     gene            15000..16994
                     /locus_tag="VFQ79_21750"
     CDS             15000..16994
                     /locus_tag="VFQ79_21750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEU0142361.1"
                     /translation="MTPAASNAPEMTVKAVGLGIFLAFVFGSANAYLGMRAGQTVAAT
                     IPAAVVAVAVFRIPAFRGGLLEQNITRTAASVGEALVAGAIFTIPAFMMVDVGGVRLW
                     TDMRAHYWDATMILLAGGLIGVFFIILLRRPLVVDADLPWPESVASYEIVRSSESASE
                     APRYIFGAMAFGGVVQILKSGKGLQVFQEFSEGFLNFPRSVIRHFNFDKAPIGDVTHL
                     GGIPWSTPSLSPALIGIGYIIGPTLASINVSGGIIAWWVLIPLLLFFDPDLPRRLGTA
                     AEADWTVISYSVWFNVVRPIAVGTMLVGAGYTLFSMRSSIARSLTGAFSASAAAARED
                     ATIERTERDIPPKWVIAAILILLVPITLLYYYFTGGWMAAIVAALVMSLTGFLLSAVG
                     GYLVGLVGSSNQPLSGLTLSALVITALLMVAIGVKGIAGVGAVLGVASVVACAASVSG
                     SLIQDLKAGHLLGGTPWKMQVVEIIAVVLLAFFLMGPIIALHQANLDTGGIGGRALPA
                     PQAGLMAQLAKGIVGGQMAWGLLAAGAAFGFALVLCGARAPMLVAVGMYLPFDTSSAI
                     FIGGLMKLVADRVTRRYEPERRQKFEERGTLLASGLIAGEAIVGILLAVFFLTGISSI
                     TWLLTGSDQFDFVPAWGGWLSLIGFASIGYVLIRLPARKL"
     gene            complement(17170..18240)
                     /locus_tag="VFQ79_21755"
     CDS             complement(17170..18240)
                     /locus_tag="VFQ79_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0142362.1"
                     /translation="MILAPSGFNKDNHANRSLTFAAQKRLRSRERERADLANWHGYFL
                     TVPNAPVTRRTLLRAAGLAAVSAGKGYAEPIPIIDTHIHLFDTTRPQGVPWPDPKNTV
                     LYRPALPDRYRKIAVPLGIAGAIVVEASPWLDDNQWVLDVAAKDPIVVGVIGNLDPGE
                     RDFGKHLERLQRNPLFRGIRYGNLWGRDLGAGLDKPEFVSSLKLLSQARLTLDTANPT
                     PALLSDVIRLTGLIPELRVVIDHLPARRNYHAHLREIGSRPRIFAKLSAVFRRIDGQV
                     PRDLSVYRQTLDELFGVFGEDRVLYGSDWPNSDQLRPYGDVLALVQEYFSGKSTTAAQ
                     KYFWRNSVRAYEWVKRDPSQPG"
     gene            complement(18212..>19560)
                     /locus_tag="VFQ79_21760"
     CDS             complement(18212..>19560)
                     /locus_tag="VFQ79_21760"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEU0142363.1"
                     /translation="ASLWAQSEAVVIRPVEIDDVLVNPGMGIQTFQRYNGDPINPGLR
                     WSEAGPDTRIKAASAKPDYPDSSIAYFRWFWSQIEPAQGKYRWDILDLALEEARKHNQ
                     KLAIRLMPYDEGHPLPEWYRKSGARRANTDDDKEWKTWAPDADDPLYLKHWGGLILAA
                     GARYDGHPFLDSVDISTVGYWGEGWGPYLPSWERQEELIDVNFEAFQRTPLLVNFDEP
                     RALAYATQKGSGWRLDCWGDMGNYWESWGRHLSHMLDVYPQQVVRAGASRVWERSPVS
                     LETCWVPGYWRQKNWDLDYILEQALRWHVTSVNIKSSAIPPDWKQKFREFEKRIGYRF
                     VLRRFEYPQVVKAGEMMPVKSWWVNKGVAPPYEDFVLAFGIGDSVIRTSANLKTWLPG
                     DQVFDGSLFLPPELKRGEHKIRVAVLDGRTGAPAVKLAIQGLQPDGWYDLGTIRIQ"
BASE COUNT         3844 a         5916 c         5754 g         4046 t
ORIGIN      
        1 tgttcactat gaatgaattt ggcaagctgc tgcggatcgg ctttttgcag cgcgtcgaag
       61 gtggcgacct ctgcgcccag gctcctcact acgcgatcga ggagtctttt cgcctgctcc
      121 gggccgaagg cgttgtgcag gaccttccgc gcgtagtcga tgccgccttt gagcacgtaa
      181 tcgtgcgcca tcgacatctc atagaattct tcgagtacgg actcggcctg atcggtgggc
      241 accgaggaga ttttagcgat ctcgcggctg atgagttgaa cttcgtcctc gtcgagttcc
      301 ttaaggatct cgctgcttgt ctggtcaccc agcgtgacca tcaaaacggc ggctttgcgt
      361 agacctgaca aaactaccgg ccggtcggca tttgcaatca tctgctagtt ctctcgtcgt
      421 tcagccacgt gcgcaccacg tgagccatgg atgtaggatc tttcttggcg ccatcgccga
      481 tgtgccgggc cagcacctcg gtcttctttg tcgagacggg ttgaatcttg agcgagctga
      541 gcgcttcatc gttcatcttc tgctgcaagg cggcccgttg ggcgagagcc gcctccatgc
      601 tcatggatgc atcgttctgg gaatcgcctt caatactttt ctgtccctgg ccggaagcta
      661 cctcctttgt catttcaacc tgcttccgtg ctcgttttcg atttcgagat cttctgaaga
      721 gaaacagcat cagtgccagc agaacgagga caacgccggc accggcgcca atcagaactg
      781 ctccgttcgt ctggccaact atcttttgaa gccacgccgg caactgtaaa gtacccggct
      841 gtgccggagg tggcgaatcc ggcttgggcg gctcccaatt ggctgtggtt tcaaacggca
      901 ggctctccac aatcaactgg tcgccccggt ccggagtgaa tccgacggct gcggcaacca
      961 gatcccgaat cacttttagt ttctccggcg gcggcggttc caacacacgc ttcgcatctg
     1021 cgcccgagcc ctcccaccgc acgtcctgat ccaccagtaa cgatacagag actcgtttga
     1081 taccaccctg cggcagcttg acgtggcgga ccgtccggct cgactgatag gtgatgttct
     1141 cggtacggcg gctcaagcct ccggcggatg agaccggacg ggaaggcggc cgcggcagat
     1201 tcgatgcaac tcccggggtg ccggatgtgc cgccggttcc ggtggcatcc tcggtccttt
     1261 gagaggtgac cattacggac cggttgggat cgaaggtttc ttcactctgt tccccgcttg
     1321 tgaagtcaca atcgacaaag acgcccgccc ggaagcggtc cgggccgagc agcggctcga
     1381 gagtagtgtt gattttcgta atcagatccc gctcgatcct ctgccggtat tcgagtgatg
     1441 cctcggaggg ctcagccgcg tccgggttgg cgggccgcgg ccggttcagc aggttgccgc
     1501 gaacatctaa caccgaaacc atttccggag acaaaccttc aacggcgctg gcgagcaaat
     1561 ggcagatgcc cgcgatgttc tgggccgaga gcttgctgcc aaagcggagc ttgagcatta
     1621 cgctggcttt ggccggctgg cgcgattcga cgaaaaggga atctttcgac agcgtgatat
     1681 gcactcgcgc atgttccact tcggaaagag aaatgagcga ccgttcgagt tccccttcga
     1741 gtgcgcggtg ataattcacc tgttcggcga attcggtcaa tccgaagttg gtttgatcga
     1801 acaattcaaa cccgatgcgg cctgttttcg gaagcccggc ggcagccatt tgcaggcgca
     1861 actcggcgac ttttccagaa ggaaccagga cggtacttcc gttttcttcc agcctgtact
     1921 caatgtttcc ttccttcagc ttcgcaagca ccgccccggc gtcttcagac gacaatccgc
     1981 tgtaaagcag cttgaaatcg cgttcgcggt tccactttgc cgctccgaac agcgcggcga
     2041 tgacggccag ggccacgata cccgtcgtga ttcgctgccg catgctgagg ctgaggaaga
     2101 gattcttgag ttgctccata ttccggtcac gttccgttcc gtttcaacgc gcggccgcta
     2161 tacctgcatc cgcatgactt cctgatacgc ctggacaacc ttgtttctca cttgaagaaa
     2221 catctcgaac gccagttcgg cttgctgtgt ggctaacaca gcctggtgca cctcttcggc
     2281 ttccccggac aggaacttgt cgatgctttg atgagatacc tgacggagtt cgttcacctg
     2341 cgagatggcg ccttcgagcg ccacttgaaa tccgccctgc tgctttcccg cacccggaac
     2401 caccgtggag gggatgtttg gaatgccagg cacattgaaa gatattgggt tcatcgcatt
     2461 agctcaatgg aacgatggat catgtctttc accgcgctca tggcggcgat attggcctgg
     2521 taggcgcgag aggcacccat caagtccacc atgtcttcgg ccgggttgac gcgggggaac
     2581 gccacgtagc cgtttgcatc ggcatccggg tgccccggca tgtatcgttt ttccagatcg
     2641 cgcgtgtcct ggaccacctc ggaaacagcc accccggtca cgccggcccc aatctccgac
     2701 tggaaaaccg cggagaacgg cgaggtctga gactcggtgg caaataccac atccttacgt
     2761 ctgtagggtc caccttccgg agtccgcgtg gtctcggagt tcgcgatgtt ctcgaccatc
     2821 acttcggccc gcgccctttg cgcggacatc cccgatgcgc taacggataa cagagaaaaa
     2881 agactcatcc gccccttcct tcctgaatcg ccgaacggat ggtccggatc tgagagcgca
     2941 atagattgga agccatgtta aaccgcagtg cgttctcgga tagcagccgt agttcgcgat
     3001 ccatattcac gttgttgccg tcgtttttcg taaccaaccc gggtacctcc acgacattgg
     3061 gcaccgctcc ctcgatttga ctcatgaact caaactgaaa actgatgtcg cgggtcttat
     3121 atcccggagt gtccgcgttg gctatgttgg acgccactaa cttctggcgc gcactgagca
     3181 gatccatgta ccgctcgagt ttcactccca atgcatcgaa catcgactgg ggactatgca
     3241 agccggttgc cagagctgcg gcacgatttc gaagagcaga aggcgttacc ggctcagcac
     3301 gagctacttc tgtccaaacc cgtatttcgc gggaacccgg aggcgcgtca ggattttggc
     3361 acagacgaat ttacggctta ttctgaggtt cgtacatccc gggataagcc gtccgcattt
     3421 catcggcaat ttcaagcgct gttccaattt gaaccagcgg tccggttccg atcccaggac
     3481 agtacacggc ggtggctttc caggcagcaa cattctccac caactccggc cagaacggga
     3541 aaagacggca ttgcgccggc ttggcgggat ggatccggca accggtcccg ttcaggaaat
     3601 gacactgcga gttccgaggt ttccgcagcc ggatgaaatt gcgcgtccga taaacgtact
     3661 tcgcttcgaa cgcggcaggc gacataccca aaaatccggc agccttcggg atgtcggctt
     3721 cgctcaggta cacgtacccg tgttgccggc agcacgccgt acaacctgct tgacatgcaa
     3781 atcgaagccc gtccatcgaa accaggatca cacgttttga ttcccgggcg ttccccttcg
     3841 atactgagtc tatgcggcga acctcacgaa gggaattctg taaaacaatt ccatcagggc
     3901 tggcgttggc cgcggcggcg aacgcacagc agccggatgg agtgctgctc gaagcgtcgg
     3961 cgtttgcgga ccatggcgga tgggttctcg atcagcagag catcgatcgt atcggttcgt
     4021 cctatcttct ggcgcacggt tgcggacggc cggtccagga tgcggcaacg gtcgtggagt
     4081 tcccatccac cggtgagtac catctgttcg tccgcacgcg agactgggtc ggtccctgga
     4141 aaggtgcaaa cgtcgcgccg gaactgcggg ccgaaggttc tcccggcgtg tttcgcttgt
     4201 tgattgaagg cgaaccagtt gagacggtct tcggagcgga aggggcggac tggcattggg
     4261 caccgggagg tccggtccgg atcacgaaaa agaaggtcac ggtagctctt cgcgatttga
     4321 ccgggttcga cgggcgttgc gcagcgatct ttttttcccg aaccccgaat ccccaattgc
     4381 ccgatgcccc ggatgcggta cgcgcctggc gcagccgctt gacgggcggg cccgcggaac
     4441 ccgcccctgt cgggcgattc gatttcgtcg tggccggcgg tggtgtggcc gggatatgcg
     4501 ccgcggtcgc cgcagcccgg cacgggctaa aagtagcgct gcttcacaac cggccggtgt
     4561 tgggcggcaa cagcagctcg gagatacgcg tcagtccctc ctccgtgcgc aatatcgagc
     4621 cctatatcgg gctgggaagc attctcggcg agttcgactg gaaccgtccg gtggagcccc
     4681 ctcaggctcg cgtatttcag gacggcgacg atattcgcct gaagctggtc ggcgacgagc
     4741 ggaacatctc gctctttctg aaccaccacg tttataaggt ggaaaaagag ggagtggtca
     4801 tcaaggcggt tcacacgcag cacgtgctct cgggtgcgtt gtcgcgattc gagggcgcat
     4861 tgtttgctga ttgctccggt gacgcaaacc tcggtttttt agcgggcgcg cactggcgga
     4921 tgggccggga aggccggccg aaaaccgggg aaacgcgagc gcctgaaaag gcggacggac
     4981 aggtcatcgg ctccaccttg cactggtatt cccgaaccac ggaaaagaaa acgctgttcc
     5041 cggacgcccc ctgggctctt ccgtttacgg aagaaaaatg ccagttcgtc accgagggtg
     5101 gctggaattg ggaaacaggt tttcatctcg atcaggccaa ccaaaccgaa ttgattcgcg
     5161 accacaagct tcgcgcgata ttcggaaact gggcgtacca gaaaaacaag agcgaacgca
     5221 aggactggta tacggacctg gaactggcct gggtcggcta cgtgctcggc aagcgggagt
     5281 cgcgccgcct gatgggcgat ctggtgtact cgcaaagaga cctggatgcc ggaaccgaat
     5341 accccgacgg ttgcatcgcc tgcaattggg gaatcgatat ccatgttccc gatccgaaga
     5401 accagaaaca attcgccggc tgggcattcc gcgccgtcgc cgaacactac gacaaagggc
     5461 gcgcgcccat gcgatggctg ccgtaccggt gtttatactc gcgcaacata ccgaacctgt
     5521 tcatggccgg acgaaatgtc agttgcactc acgttgcgtt cgcctggttc cgcacccagc
     5581 gaacgaccgc gatgatgggc gaggccgtcg gcctggccgc ggcgctgtgt atcgatcgaa
     5641 aatccacacc gcgcggcgtg tacgcggagc atctgaacaa atacaaggaa atgctgaaaa
     5701 aaggcgccgg caaggtcacc gccggcagtc caaagtcgta gaccggcccg aatcacaacc
     5761 cgagcctttg tttcacttcc gaagcgcgat ggcgtgcaac ggtgagttcg gtatggttgg
     5821 cgtccttgag ccgcacaatg taacttcccg cgaacgccgg ggcgatatat tgcacccatt
     5881 cccgattcag caaggtcgcg cggtgtatcc ggatgaagcg cttcgggtcg agccgctgtt
     5941 ccaggccggc cagcgtgtcg tccacacaat gcggtttccc gccggccatc gcataggtga
     6001 gcttgtcctc cgcatagaaa tgggtgacgt gcgccagatc gaggaaacgg atacgctctc
     6061 ccacgcgcgt tgcaattcga tccggatagt cccgggcacg ctgccgcaag gacgaagcga
     6121 cctcctcgaa cagcttccgg aaatccgcgg cggcaacaag actgccgcat agccgctcga
     6181 tcttgttcaa agcgcgctcg agctgcaacg ggtcgatcgg cttgagcaaa tagtcgatcg
     6241 agttcatctc gaaggctttc agcgcgtact gatcatacgc cgtagtaaaa accacgaccg
     6301 gttgagaagg ccgtttcgca agcagctcga aaccggtgat gccaggcatc tggatatcga
     6361 gaaacaatac atccacctga tggtgcgcga gaaactccag cgcggcctgc gggtccgtgc
     6421 tgctccccag gatttcaatc cgccgcgtgt tcgcgagcag gcgccgcagg cgatccaccg
     6481 ccagcggctc atcatcgacg atgtacgcgc tcaagctcac ccggcggcaa ctccttcgcg
     6541 ttgtgcaggg atctccagtg tcacgacgca ccacccatca cggctcccca cctggaggga
     6601 tgccctgggg ccaaacaaga cgtcgagccg cgacaccagg ttgtcaatcc catgacccgg
     6661 gtccacgttg gccaggctga agcaagggcc gctattgcgc acttccatcc ggatcgcgtt
     6721 cgcactcttt tgtgcgatga cctcgatctc accgccttcc ggcttgggtg cgattccatg
     6781 cttaaccgcg ttctccacca gggactgtat ggacagtggc ggcaacagga attccgcggc
     6841 ttgcggtata tcgaagcgat aacgcagccg ttctccaaac ctcgcctttt cgatgtcgag
     6901 gtaggctttg acgatttgca tctcttcttc gagcgggatg agcgcccgcg gcgccgcgtc
     6961 gagcgagctt cgcagtaatg tggcgagctg ccccacaatc tcctcggcac gcgcaggcgc
     7021 caccggaatc agcgagctga tcgagttcag tgtattgaag agaaaatgcg ggtggattcg
     7081 cgattcaagc gacgacagcc tcgcctcggc ggccagcttg cgtgcacgct cctcttccag
     7141 ctctttctcc tgcagccttg cgcgcgtctc tcgcagccgc gaaacgagcg attcgtagaa
     7201 gaaagcgctc atcccaaaca cgagcaccag caggcaggca gtccgcgccg tagaccagta
     7261 ctcctgccaa aaaggccggg gcggcaggag tccggccacc caaagcagcg cgctgccaac
     7321 caccgcacca gccaaagcag cgacaaggag taccaccacg taggccgatg ccagtttgac
     7381 accatgcctt cctatccggc gctcgacagg cggcagcagc aaattggcaa gcagaccgat
     7441 gcatgtcgag tggataatcg tgtatgcgaa tacccggccg gccagtgtgt gccgccaatc
     7501 ggatccaaag gccactacca cgaacggtgc cacaaccgca ccgcccacgc atatggctaa
     7561 cacccgcaca atggatggcg gcttcctcaa gtcagcagca agcatactac tctacttatt
     7621 atttacaggc tcaaatacgc ccattcggaa aatgttaact acccaaggtt ttttctggtc
     7681 ctggacactc atctctccca ccaccccttc gttaagcgtg ccgtactcga ttcgtttggt
     7741 cccgagtgac acccagatct gtccccagaa attgctccca gcgacgatgc tgatttccgg
     7801 cattttcata tcgagagtgt tgaggaaggc acggtagtca atgaccgcgc actcctggcc
     7861 atttcgttgc gatatccctg tccagatcag ttgcaggtct ttgagtgtaa cggtgcctgc
     7921 gccggcaagg gacaatgcac ccgattgcgg cacatggaac ggtatgttaa gtttcaagtc
     7981 cggaaaataa tcctgaccga agttctcgat catgtgggtg tcccaaacca ggttgcgctc
     8041 gaacatcgcc gttggcggaa agccgcgaaa gaacccgggg gcgagcatgt ctttcaggct
     8101 ggcgcgggca tagctgaagc cctccatgaa atcacacttc cgcgccgggc cgaagttgcc
     8161 ggacgaacca ctactctctg caatctcgac gccgctccat cgtacgcggt cctccggcag
     8221 gtctcgcagg taaagcgctg aaacgcgctc gcgacggccg gggtttccct tggtgtcgag
     8281 atagtagtag tcgcaggtga aacgataggt ctggggtcca gcgcccttcg cgacggggga
     8341 agcagacaga gtccacttcc acttaccgcg gggttgtatt tgtgcggagg aggccacaag
     8401 tacgagcaag ctaaggatcg ctattgtctt cacttcggaa gctcctttcg aggttcagta
     8461 tgaagacaat ccctcgctct taccttcttt ttcagataga cgcgggatga tttggacgaa
     8521 gtgccggaaa gccgcgacca atggaagcgg ttgctgttcc ttacaccggt gctacattgt
     8581 taggtgatga caccggcgat tgcccgtccc ggtttgttcg gctgcgatta gccgctctcc
     8641 ttttgtctgc ctgttttaat ttctgtagca gaagtggagg agagtcatgg aaggaattcc
     8701 gcagatcgtc acatccctcc cgggtccacg cgccagcgag atcatcgagc gggatcgtga
     8761 gctgatatcg ccgtcttaca cccgggaata tccgctggtt gccgagagag gggaaggagc
     8821 catcgtttac gatgtggacg gcaaccgctt cctcgacttc aacgccggga ttgccgtagc
     8881 cgccacgggg ccctgccacc cggaagtcgt ggaagcgatt cagcgccagg ccgcgcgcct
     8941 catccacatg tcgggcacgg acttttatta cgtagggatg gtccagctcg cggagcagtt
     9001 ggcgcggctc gcgcccggcg gccgagcctg gcgtgtttat ttcggcaact cgggcaccga
     9061 ggcgatcgaa gcgtgcatga agctggcgcg ccatcactcg ggccgggaca aattcatcgc
     9121 gttcttcggc ggctttcacg gccgcacact cgggtcgctt tcgctgactg ccagcaaggc
     9181 ggtgcagcgc gccggcttcg gtcccctggt tcccggcgtc catcatgttc cctacccgga
     9241 ttgctaccgc tgcccttacg ggaaacgtcc ggacacgtgc gcggtggatt gcgtccgcca
     9301 catcgaagag gaagtgtttc gcaccactct tcccccgcag gaggttgcgg cgattgtcgt
     9361 ggagccggtt caaggtgaag gaggctacgt ggctccaccg aagaatttct tcgaccgctt
     9421 ggccgccctc gccaagacct acggcatcct gctggtgttc gatgaagtgc agtgcggcat
     9481 gggccggacg ggaaagatgt gggcggccga acacttcgac gcctcgccgg atatctttgc
     9541 cgcggcaaag ggcatcgcga gcggaatgcc cttgtcggca accatggcgc gcgccagcat
     9601 catgacgtgg ccgcccggcg cgcatgcctc caccttcggt ggcaacccgg tctcgattgc
     9661 ggcctcgctc gcaactattg agctgctcca acgggaactg atcgcgaatg ccgccaggct
     9721 gggagaacac atcctgagcc ggctccgcgc ctggccgcgc aagtttcctt tcgtcggaga
     9781 tgtgcgcggt ctcgggctca tgatcgggat tgagttcgtg cgggaccagg ggtcgaaaga
     9841 aagggctccc gaataccgca accgcattct gcaactggcg tttgaacgcg gcctgcttgt
     9901 gcttggcgcc ggcagaaaca gcatccggct gtgcccgccg ctggtgatca cgcgcgacca
     9961 ggcggatttc gctgtggaca ccctggaaga ctgcctgcta gttttgaagc gtgaaagttc
    10021 ttgacgtcgg atgcggcttg aagaagctcc ctggcgcaat cgggatcgac cgtaacccat
    10081 ccacggcggc ggatgtcgtc tgcgatctcg actcgttccc ttatccgttt cgggatgccg
    10141 cgtttgatgc cattcgcgcc gttcacgtta tcgaacacgt ctcggatgtc gtccgcacga
    10201 tggaggagtt ccacaggctg ctccacccgg gcggccgcgt ggagatcgtg acgccgcatt
    10261 acacggattt cagctctttc tgcgacccga cgcaccgctg gcatctgaac agtttttcgt
    10321 tccggtattt cggtgaagac gatgccgggt tcggatacta ctcggccgcc cggttccggg
    10381 agatttcggt gcgcgtgaag ttactaagac tgtggagggc gctcggcttc gaactgctgg
    10441 tcaaccggtt tccacgcttc cgtcggtttt gggaacacta tctttgtttc gtggtccggg
    10501 gcaaagtgat agagttcgaa tttgaaaagc ccgccggagc ctgaagcgga tcgcatcgca
    10561 tgattcaaca cttgctgggt tttgccgact ccatgcgcca ccgggcgcgc aggcgcaatt
    10621 cggatccagg gcaggagatg ggcaggcgcg gagaggacct cgcgcatcgc ttcctccgga
    10681 aggcaggcta taccatcgtg gcccgcaatt accgcacgcg atcaggctcc ggagagttgg
    10741 acgtgatcgc ctgggatggc gatacgctgg tgttcgtgga agtgaaaagc cggggttcgg
    10801 aggagttcgg ggcaccggac cgggccatcg gcgaggagaa gcgtacctcg attgcacggg
    10861 cggcgcggga ttacgcacgc cgcgcagacg tagcatggaa ccgggtgcgg tttgacgtgg
    10921 tgaatgtgat cgtggacgca cgcccggaga tcacgcatat caaagatgcg ttttattcgt
    10981 gaaatttttc caaaataaat cccctcgaat gaaaggtttg ctgctaaact aacggttttt
    11041 gcattcgttc gtgggaggtg ttttcattgc cggagcttcg aaaagacccc atcaccggcc
    11101 ggtgggtcat catcgcgaca gaccgggcca agcggcccag tgacttctca cgagaacagg
    11161 tgatccccaa agggggtgga ttctgtccct tctgccccgg caacgaactg aagaccccgc
    11221 cggaaatcct tgcgtaccgt gagaacggcg gccccaacga aaaggggtgg actctgcggg
    11281 tggtgccgaa caagttcccc gcactgcgag tggagggcga cctcaaccgc cagggccaag
    11341 gcctctacga caagatgacc gggatcgggg ctcacgaagt catcattgaa acgccggagc
    11401 acaacctcac gctcgccgac ctgcccgaaa aacaggtagc ggacgtgttc tgggccttcc
    11461 gggaccgcat cctggacctg aagaacgata cccggcttcg ctacgtcctt gtattcaaaa
    11521 accacggcga ggccgccgga gcgtctctgg aacacacgca ctcgcagcta atcgcgctgc
    11581 ccgtcgtacc caagcgcgtt caggaggagt tggatggagc gaggcgttat ttcgacttca
    11641 aagaacggtg cgtctattgc gatatcatcc gccaggaatt ggaaatggga acgcgtgtgg
    11701 tactggagac ggaccatttc attgccgttg aacctttcgc accaagattt ccattcgaga
    11761 cgtggattct gccgcgcatc catcattcac atttcgaagc gtccacggcg gctgcaatcc
    11821 agaacctggc ctggattaca cgccagacga tccggaaggt ggacaaggtg ctcgagcgcc
    11881 cgccgtacaa catgatgctg cacagcgcac ccgtgcagga ggggtcggta agctactatc
    11941 actggcacat tgagctgatt ccgaaactga ccaaagtggc cggtttcgag tggggaacgg
    12001 gcttctacat caaccccaca ccgccggagg aagctgcgaa gttcctgcgg gaagtagggc
    12061 tggtgtagat tgcggcgcct ccacgaagta taaaggagtg ccagccagca ggcggcgggc
    12121 cacaccctta gttgtttcgg tttcaaacaa acaggtccgc gaacctgctc tgccttccac
    12181 acgaaccgtt tgctcctgct ggctgatacg gtagaccatc ccggcgccgc gttctgcctg
    12241 aagagagtcc agcggccaat cgcagtcggc caccagccgg atccctcccc cggaattgat
    12301 caggatggtc aagttcgata gcgtcgaacc ggcatcggca gcggattcca ccgcttcaaa
    12361 gattcgcgtt gcgttctcca taaaccggct cacagctttc ttatcgcttc agccttccca
    12421 cttgtgagag tttttcgctt cccgtctgtt gacaaaaact caatttacat ttactctatc
    12481 gaaataatag acttaaagac tcagggagta ggtagctcat gaaaaagccg ttgcccacaa
    12541 gaggcgtcgt ggtctcgttg attctcctct tctcgctctc ggtgtttgcg caggaggcga
    12601 cggaaccgcc ggcggacctt caggaaggaa gcgccggaag tccacccacg gtatatgaaa
    12661 gcgttacggt aacggcccgg cgcgtcgagg aagaagcgca ggaagtcccg attcccgtgt
    12721 ctgtggtcag cggcgctctg atcgaccgcg cacaggcatt caatgtgaac cggctcaaag
    12781 agctgatccc gacggtacag ttctactctt ccaacccacg caactcggcc atcaacattc
    12841 ggggcttggg ctcgcctttc ggcctcacca acgatggcat cgaacccgga gtcggttttt
    12901 acgtcgacgg cgttttctac gcccgccccg ctgcggcgac gctggatttt ctggatctgg
    12961 aacgagtcga agtgctacgc gggccgcagg gaacgctgtt cggcaaaaac acgaccgccg
    13021 gcgccataaa catcacgacg cggcggccca gtttcacgcc cgaagcgaac ttcgaactca
    13081 actacggcaa ctacggattt gttcaggcga aaacgtccgt cagcggtcct ctcagcagaa
    13141 agatcgcggc gcggttgtcc ttttccggca gccaacgcga cggaatgctc tacaacgtga
    13201 ggacagagga cgacgtgaac gatctgaaca atctgggcgt ccgcgggcag gtattgttta
    13261 cgccggcgcc cagtcttttg gttctgttcg cgatagacta cacgcgccag cggccggagg
    13321 gctatgcaca agtggtggcc ggggttgcgc ccacactgcg cgcggcgaac cggcagtatc
    13381 cggggatcgc cgccgacttc gattaccggc cgccaagctt caacgccttc gaccggctga
    13441 ttgataccga tatcccctgg cgatcgaacc aggacatggg tggtgcctcc gtcaccgtcg
    13501 attgggaccg tgggccggga gtgctgacgt ccatcaccgc ctggcggttc tgggattggg
    13561 acccatcgaa cgatcgggat ttcatcggcc tcccggtgac caccatctcg gccgctccct
    13621 cgaagcagag gcaatggaca caggtggttc gctatgcagg cagaatcaca tccggcatga
    13681 acttcctggt cggcgcattc ctgttccatc agaagctcaa caccgatcca ttccacaagc
    13741 aggaacaggg cgccgccgcg gcccgttttc tcctggcacc gagcgcactg gcggcgacgc
    13801 ccggcctgct cgacggatac ggacagaacg tcaatttcga cttcagcaat accagctcgg
    13861 cggtattcgg tcagatcgac tatgggctta cgaatcgcct gcggctgatt cccggcctcc
    13921 gcgtgaacta tgaccagaaa gatgtcgtct acgatcagca gctttacggc ggcctcaaaa
    13981 caaccgaccc cgcgctgatc gcattgcagc gatccattct cgcgccgctc tcctacaagg
    14041 ccgatgccgg cgacgtcaac gtatccgggc agttgacgct ggcctacaaa gcgggcgaaa
    14101 gcgccaacgc ctatgcgtct tattcgaccg gtttcaaatc gatcggcctc aacctcggcg
    14161 gcgtgcccac ggatgcggca ggccaaccca ttacctccgc tgcgaccgtg agacccgagc
    14221 gcgtccgcca cctcgaactc gggctgaaga cccaaccgct ccggaatgtc accgcgaata
    14281 ttaccctctt caacacgggc atcaaggact tccagaccca ggtgttgaac gcgcaagtcg
    14341 gcgtgctccg cggctatctt gccaacgcgg agaaggtgcg cgtgcgcggg atcgaactgg
    14401 acgccaacgc gatggtgact ccggaactgt cggcctacat atcggccgcc tattcggacg
    14461 gcaggtatct taccttccgc gatgcgccgc ctccgctcga agagactggc gggccgcagg
    14521 tgaaggatat ctccggatcg gtccttccag gcatctcgaa gtgggcgttt tccgccggcg
    14581 gcgaatatgc aaagcgcgga agcctgctca gcacgacggg cacgtacttt gcgcgcgtgg
    14641 acaccagcta ccgttcctca ttttcctcca acccgtcagc ctcccgctat ctgatcgccg
    14701 atagttatgg cctggtgaat ccccgggccg ggttccgcag cgtagatggt tggacaatct
    14761 ccgtgtgggc ccgaaatctg ctgaacacgg actacttcga gcttctcgcc gcggcgcctg
    14821 ggaactccgg gctgtacacc ggtctgcccg gagatcgcag aacgttcggc gtgagcttcg
    14881 gctggacctt ctacagaggg tctcgccccg ttcctacgcc caccagggcg cagtccccga
    14941 aagattgata caggcagccg gagcagagcc ccccggcggt tccctgttaa gatcgcctca
    15001 tgactcctgc cgcgagcaat gctccggaga tgactgtaaa agctgttgga ttaggcatct
    15061 ttctcgcctt cgtattcgga tccgccaatg cctatcttgg gatgcgcgca ggccagaccg
    15121 tggccgcaac cataccggcc gccgtagtgg cggtggccgt attccgcatc ccggcttttc
    15181 gcggcggcct gctcgagcaa aacatcacgc gcaccgcggc atcggtgggc gaagcgctgg
    15241 tagcgggagc gatcttcacc atcccggcat tcatgatggt cgatgtgggc ggcgtacgtt
    15301 tgtggaccga catgcgtgcg cattactggg acgccacgat gattctgctg gccggtggat
    15361 tgatcggggt gttcttcatc atcctgctgc ggcggcctct ggtggtggac gcggaccttc
    15421 cctggcccga aagcgtggcg agctatgaaa tcgtccggtc gagtgaatcg gccagcgagg
    15481 cgccacgcta tattttcggc gccatggcat tcggcggtgt cgtgcagatt ctcaagagcg
    15541 gcaaaggcct gcaagtgttc caggagttct cggaaggctt tctgaatttt ccccgttccg
    15601 tcatacgcca cttcaatttc gataaggcgc ccattggcga cgtgacgcat ctcggcggga
    15661 taccgtggtc cactccgagt ctgtcccccg cgctgattgg aatcggctac atcatcggac
    15721 ccacgctggc ctccatcaac gtgtccggcg gaattatcgc gtggtgggtg ctgattccgc
    15781 tgctgttgtt tttcgatccc gatttgccgc gacgcctcgg cacagcagcg gaggccgatt
    15841 ggaccgtgat ttcttatagc gtctggttca acgtagtgcg ccccatcgcc gtgggcacga
    15901 tgctggtagg cgccggttac acgctgtttt ccatgcgcag ctccatcgcc cgctctctga
    15961 caggcgcgtt ctccgcttcg gcggcagccg ccagggagga cgccaccatc gaacgcaccg
    16021 aacgggatat cccgccgaag tgggtgatcg cggccatcct gattttgctg gtgccgatca
    16081 cgcttctcta ttactacttc accggcggtt ggatggcggc gattgtcgcg gctctcgtga
    16141 tgagcctcac cggctttctt ctctccgctg tcggcggcta tcttgtcgga ctggtgggaa
    16201 gctcgaacca gccgctatcc ggactcacgc tttccgccct ggtgatcacg gcgttgttaa
    16261 tggtagcgat cggcgtgaaa ggaatcgccg gcgtcggcgc agtgctgggc gtcgcttccg
    16321 tggtcgcgtg cgcggccagc gtttccggct ccctcattca ggacctgaaa gccgggcatc
    16381 tccttggagg aacgccctgg aagatgcagg tggtagaaat catcgccgtg gtgctgctgg
    16441 cgttctttct gatgggtccc atcatcgcgc tgcatcaggc gaacctggat accggcggaa
    16501 tcgggggccg tgccctacca gcgccacagg ctgggctgat ggcgcagctt gcaaagggga
    16561 tcgtaggagg gcagatggca tggggtctgc tcgcggcggg cgcggcgttt ggtttcgcac
    16621 ttgtgctttg cggcgctcgc gctcccatgc tggtggcagt cggcatgtac ctgccgtttg
    16681 acaccagttc cgccattttt atcggcggat taatgaagct cgtcgcggac cgtgtcaccc
    16741 ggcgatacga gcccgaacgg cgtcaaaagt tcgaagagcg aggcacgctg cttgcctcgg
    16801 gtttaatcgc cggagaggcg atcgtgggta tcctgctggc ggtcttcttc ctcaccggca
    16861 tctcttccat tacctggctg ctgaccggct cggatcaatt cgatttcgtg cccgcatggg
    16921 gcggatggct ttcgctgatc gggttcgcga gcatcggcta cgtgctcatc cggctcccgg
    16981 caagaaagct gtagacgcat ttcaacgcga ccgctccgaa acggagccgc gcgcgtcaga
    17041 agctgtgaag aaattgaatt tagccacgga tggacacgga tgaacacaga taagttccct
    17101 gttttcaatc tgtatttatc tgtgtccatc cgtggctctg aatctttcac accttctaag
    17161 tgagcggcat caacccggct gcgatgggtc gcgtttcacc cattcgtagg ctcgcacgga
    17221 gttcctccag aaatacttct gcgccgctgt cgtgctcttg ccgctgaagt attcctggac
    17281 caatgccaga acgtcgccat acggccgcaa ctggtcgctg ttcggccagt cgctgccgta
    17341 taacacccgg tcctctccga acacgccgaa cagctcatcc agcgtttgcc gatagacact
    17401 caggtcccgc ggaacctggc catcgatgcg gcgaaagacc gccgagagtt tagcgaaaat
    17461 ccgcgggcgg ctgccgatct cccgcaagtg agcgtgatag ttccgacggg cgggcaggtg
    17521 atcaatcacc acgcgcaatt ccggaatcaa gccggtgagc cggatcacat ccgatagcag
    17581 cgcgggcgtg gggttcgccg tatccagtgt cagacgcgct tgcgagagca gcttcaggga
    17641 agagacgaat tccggcttgt ccaagcccgc gccgagatcg cggccccaca gattcccata
    17701 gcggatgccc cggaacagcg ggtttctttg cagccgttcc aggtgcttgc cgaaatcccg
    17761 ctcgccgggg tcgaggttcc cgataactcc cacaacgatc gggtctttcg ctgccacatc
    17821 gagcacccat tggttgtcat cgagccacgg gctggcttcc actacgatgg cacccgcgat
    17881 tccgagcggt accgcgattt tccggtagcg gtccggcagg gcgggacgat acagcaccgt
    17941 attcttcggg tcgggccacg gcacaccctg cgggcgcgtg gtgtcgaaca ggtggatgtg
    18001 agtgtcgata atcggaatcg gctcggcata gcccttaccg gccgataccg ccgccaatcc
    18061 ggccgcacgc agaagcgtcc tgcgggtcac gggtgcgtta gggaccgtca ggaaataacc
    18121 gtgccagttc gccaagtccg ctcgctcacg ctcgcggctc cgtaatcgct tctgagccgc
    18181 gaacgtaagt gagcggttgg catggttatc cttattgaat ccggatggtg ccaagatcat
    18241 accagccgtc cggttgaaga ccctggatgg cgagttttac agccggagcg ccggtgcgtc
    18301 catccagcac ggccacacgg atcttgtgct ctccgcgttt caactccggc ggcaggaaaa
    18361 ggctgccgtc aaatacctgg tcgcccggga gccatgtttt gagattcgcg gatgtcctga
    18421 tgacggaatc gccaattccg aaagcgagca cgaaatcttc atagggaggc gcgactccct
    18481 tattcaccca ccaggatttt acgggcatca tttcgccggc tttgacaact tgtggatatt
    18541 cgaatcgccg cagaacaaac cggtagccta ttcgtttctc gaattcccgg aacttctgtt
    18601 tccaatcggg tggaatggcg ctcgacttga tgttcacgga cgtgacgtgc catcgcaaag
    18661 cctgttcgag gatgtaatcg agatcccaat tcttctggcg ccagtagccg ggaacccaac
    18721 aggtttcgag tgaaacggga ctgcgctccc acacccggct cgcccccgcg cgcaccacct
    18781 gctgcggata gacgtccagc atgtgcgaaa ggtgccggcc ccagctttcc caataattgc
    18841 ccatatcgcc ccagcagtcc aaacgccatc ccgagccctt ctgcgtggca taagccaaag
    18901 ccctgggttc gtcgaaattc accagcagcg gggtgcgctg gaatgcctcg aaattcacgt
    18961 ctataagttc ctcctgccgc tcccaactcg ggaggtaagg accccaccct tctccccaat
    19021 agccgacggt cgaaatgtcg acggaatcaa gaaacgggtg tccgtcatag cgggcaccgg
    19081 cggcgagaat gaggccgccc caatgcttca gatagagcgg atcgtccgcg tcgggcgccc
    19141 acgtcttcca ttccttgtcg tcgtccgtgt tggcccgtcg cgcaccggat ttgcgatacc
    19201 actccggcag cggatgccct tcgtcgtacg gcatcaagcg aatggcaagc ttctggttgt
    19261 gcttcctggc ttcttccaat gccaggtcga ggatgtccca ccggtatttt ccctgcgccg
    19321 gctcgatctg gctccagaac cagcggaaat aggcgatgga ggagtccgga taatcgggtt
    19381 ttgcggaggc tgctttgatt cgtgtgtccg gccccgcttc cgaccaacgg agtccgggat
    19441 tgatcgggtc gccgttgtag cgctggaacg tctgaatgcc catgccgggg ttcaccagaa
    19501 cgtcatcgat ctccaccggc cggatgacca cggcttcgga ctgcgcccac agcgacgctg
//
LOCUS       DASTMK010000525         5114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_474134, whole genome shotgun sequence.
ACCESSION   DASTMK010000525 DASTMK010000000
VERSION     DASTMK010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474134"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFQ79_21765"
     CDS             <1..552
                     /locus_tag="VFQ79_21765"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEU0142364.1"
                     /translation="SSYEGGVREPFIARFPGRVPAGRVSNGVASTMDVLPTVARLCGA
                     PLPEKPLDGIDIWPLLAGEKESIDREALLYFDNYHLQCARWRNWKLHIARYNVMAYNP
                     APQGGRVNLPLKNSELYNLDLDPSESYDVAPENPQVVKTILARAEVLIAGFPEPVRMA
                     WEETRKRNVGNSATGALPRILRN"
     gene            567..1205
                     /locus_tag="VFQ79_21770"
     CDS             567..1205
                     /locus_tag="VFQ79_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0142365.1"
                     /translation="MRIFWTVLLTGAFSVFAFSAEPGFVSLFNGKNLNGWQLVNGKGP
                     GYVVKNGLLVCPADGGGNLFTDKEFDNFIFRFEFRFEPGGNNGVGIRAPLSGDIAYQG
                     MEIQILDHDHERYKGRLKPTQYHGSVYDVFPAKTGHLKPAGEWNSEEIVADGRRIKIT
                     LNGAVILDVNLDDMKDPAVLAKHPGLKRTGGHIGFLGHGTLVEFRDIRVKTI"
     gene            1253..1627
                     /locus_tag="VFQ79_21775"
     CDS             1253..1627
                     /locus_tag="VFQ79_21775"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU0142366.1"
                     /translation="MYAGDCTRNETAVLDRTAAIDRVGGDLALLQEVAQLFLDHTPEM
                     LAAIKKAIGDSDAAALQLAAHSLKGCVGTFGAAQAYRAALNLEVIGQNGRLGDAVDAL
                     SKLETALDELTPAIATLTAPLP"
     gene            1696..1944
                     /locus_tag="VFQ79_21780"
     CDS             1696..1944
                     /locus_tag="VFQ79_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142367.1"
                     /translation="MPKPGTEITPREDKSTKPSGPRIRCPFCGWSPGEQDFWSCTCGH
                     VWNTFETGGVCPSCLHQWTSTQCPKCHRWSPHSDWYEY"
     gene            2010..2858
                     /locus_tag="VFQ79_21785"
     CDS             2010..2858
                     /locus_tag="VFQ79_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEU0142368.1"
                     /translation="MADTEVTEIVCAHSPDSDDAFMFYGLATKKVRSRSLSLRHVLED
                     IESLNRKAMQGSYELTAISYHAYPYVADKYVLMTSGSSVGDGYGPILVSSHPMAPEEL
                     KGKRIAIPGKMTTAFLALQLFQPDFQCVEIAFDKILDAVREHTVDAGLVIHEGQLSFA
                     KGGFHQVVDLGRWFKKSFDLPLPLGANALLRSLPGGVQRECTRLMRESIQYSLDNHEE
                     ALNYALQFARDLDVGLAEKFIGMYVNHYTVDCGELVPRAAQKLLDMGHEAGLIPHRVE
                     LEFIRS"
     gene            2904..4313
                     /locus_tag="VFQ79_21790"
     CDS             2904..4313
                     /locus_tag="VFQ79_21790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0142369.1"
                     /translation="MKRPYDSRLTRPDMSKLTVLKLGFGAMVALLVFSAVEGYRIQEQ
                     SSRNTAAIYQRFVETGEQLSQMRRLLFAASLTIRDYLFCKEAARSAAFRTELRELAAQ
                     TRQALQTLEHSIGHRQTGELREMINGFLGTIELAIPWPDEQRATAGFAFIQSEIAPRR
                     GAASGVLRELMSVNQQLLETSRQQFAASWRAAAARLSIMLVLCIVIGVVVAHASVGHV
                     SRLEAQSIRRFEEVMQAKEELARLSARLLQVQEDERRRLSRELHDEIGQTLTALRIEI
                     SHARDAWLAGSPSLSARLNEAHALAERTVNTVRDISLLLRPSLLDDLGLEPALQQQVE
                     DFSRRSGIRCTLEQTRLDDTLPDSVKTCVYRAVQEALNNCEKHSGASWVRIQALQDGP
                     LLSVEIEDNGVGFEVNRHGAALGSGLGLIGMRERVAALGGRLTLVSAAGEGTKLSLTV
                     PLQGQAGSRIPVRAEAAEN"
     gene            4314..4961
                     /locus_tag="VFQ79_21795"
     CDS             4314..4961
                     /locus_tag="VFQ79_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0142370.1"
                     /translation="MPTNILLADDHCILRQGLRRILESYPDFTVVAEAGSGRQAVELA
                     REHQPDVAIVDIAMGELNGIDATAQIIRYSPRTAVIILSMHGDERYIVRALNAGAKGY
                     LLKDSVEQDLVQAIQSVCADRPFFSPAIRQVLAERQASASVPGHVEDRYDLLTGRERQ
                     IYQLLAEGRSNKDIASLLVLSLHTVETHRTRIMEKMNVHSAAELVLSAVRRGIVR"
BASE COUNT         1021 a         1512 c         1547 g         1034 t
ORIGIN      
        1 agctcctatg aaggcggcgt gcgcgaacca ttcatcgcgc gattccccgg gcgcgtgccc
       61 gcgggccggg tttcaaacgg cgttgcatcg acgatggatg tgctgccgac ggtggcccgg
      121 ctgtgcggag cgccgctgcc cgaaaagccg ctcgacggca tcgacatctg gccgctgctc
      181 gccggagaga aagaaagcat cgatcgggaa gcgctgcttt acttcgacaa ctaccatctc
      241 cagtgcgcac gctggcggaa ttggaagctg cacatcgcca gatacaatgt gatggcctac
      301 aatccggcgc cgcaaggtgg ccgtgtgaac ctgcccctga agaattctga attgtataat
      361 ctcgacctcg atccgagtga gagctacgat gtggctccgg aaaatccaca agtggtgaaa
      421 acgattctgg cgcgcgcaga agtcctgatt gcaggttttc ccgagccggt gcgcatggct
      481 tgggaggaaa ccagaaaacg caacgtagga aactccgcca ctggcgccct gccccgcatc
      541 ctccggaatt gagtaaagta actgtcatgc ggattttttg gacggtcttg ctgacaggtg
      601 ctttcagcgt gtttgctttc tctgccgagc ccggctttgt ttccttgttc aacggtaaga
      661 acctgaacgg ctggcaactc gtgaatggca aaggccctgg ctacgtcgtc aaaaatggct
      721 tgctggtttg ccccgcggat ggcggcggca atttgttcac ggataaggaa ttcgacaact
      781 ttatcttccg gttcgaattc cgcttcgaac cgggcgggaa caacggcgtg ggaattcggg
      841 ctcccctcag tggtgacatc gcctaccagg gcatggagat tcaaattctc gatcacgacc
      901 acgagcggta caagggcagg ttgaaaccga cccagtacca cggctccgtg tacgatgtgt
      961 tccccgccaa aaccggccat ctaaaacccg ccggcgaatg gaattccgag gaaatcgtgg
     1021 ccgacggacg ccgcattaaa attacgctga acggcgctgt catcctggat gtcaatcttg
     1081 acgatatgaa agacccggcc gtgcttgcca agcatcccgg gttgaaacgg accggcggcc
     1141 atatcggctt tctggggcac ggcacgcttg tcgagttccg ggacatccgg gtgaaaacaa
     1201 tatagttccc gttccgctaa agccggcggc gaaagttgcc gatatccaaa gcgtgtatgc
     1261 cggcgattgt actcgaaacg agactgcggt tctcgacagg actgctgcca tcgaccgtgt
     1321 cggcggcgat ctggcgttgt tacaggaagt tgcccagctt tttctcgatc acactcctga
     1381 aatgttggct gccatcaaaa aggcgattgg agacagcgat gcggcagctc tgcaacttgc
     1441 cgcgcacagc ctgaagggat gcgtaggaac gttcggtgcc gctcaagcgt accgggcggc
     1501 gctcaatctt gaagtcattg gccagaacgg ccggctcggc gatgccgtgg acgcattgag
     1561 caaactggaa actgcgctgg acgaactcac tccagctatc gcgacgctaa ccgcaccgct
     1621 gccgtagccc cacagcgccg ccttctggcg tatgctgcaa agaggggccc gttgcctgta
     1681 cgcccgagcg ctgccatgcc gaaacccggg accgagatca cgccgcgcga ggacaaaagc
     1741 acaaaaccct ccggtccgcg catacgttgt ccgttctgcg gctggtcgcc cggcgaacag
     1801 gatttctggt cctgcacttg cggccacgtt tggaatacct tcgagaccgg cggcgtctgt
     1861 ccttcctgcc tgcatcagtg gacctctacg cagtgcccga agtgtcatcg ctggtcgcca
     1921 cactccgact ggtacgagta ctgaggaggt tccagagggc atctccggac gctccgtctg
     1981 ctatagtgtg taagtctttg gctcatctta tggctgatac tgaagtcacc gaaattgtgt
     2041 gcgcccatag tccggattcc gacgatgctt tcatgttcta tgggctcgcc acgaagaaag
     2101 tccggtcgcg ctcgctcagt ctacgtcacg tgttggagga cattgaatcg ctcaatcgga
     2161 aagccatgca gggctcctat gagctgaccg cgatctctta ccacgcctac ccctatgtcg
     2221 ccgacaagta tgtgttgatg acaagcggtt cgagcgtggg ggatggatat gggccgattc
     2281 tcgtatcgag tcatcccatg gcgcccgagg aactcaaagg caaacggatt gctatcccgg
     2341 gcaaaatgac caccgctttt ctggcgctcc agctttttca gccggacttt cagtgcgttg
     2401 aaatcgcctt cgataagatt ctggacgcgg tccgcgagca cacggttgac gcgggccttg
     2461 tcatccacga ggggcagctc agcttcgcca aaggcggctt ccaccaggtg gtcgatctgg
     2521 gccggtggtt caagaagagc ttcgatcttc cgcttcccct cggagccaac gctcttctgc
     2581 ggtctctgcc cggcggtgtt cagcgtgaat gcacccgtct gatgcgggaa agcattcagt
     2641 attcgttgga taaccacgaa gaggccctga actacgcgct ccagttcgct cgcgatctcg
     2701 acgttggcct ggcggagaag ttcatcggga tgtacgtcaa ccactacacc gtcgattgcg
     2761 gggaattggt cccccgagcc gcgcagaagc tgctggacat ggggcacgaa gccggcctga
     2821 ttccgcatcg cgtggagctc gagtttatcc gttcgtaaaa cctccgcctg gaattctggt
     2881 agtctctgtc gaagagggtc ctcgtgaagc gtccgtacga ttcgcgactc acgcgcccgg
     2941 acatgagcaa gctcacggta ttgaagctcg ggttcggggc catggtggcg ctgctggttt
     3001 tttcggccgt ggaaggctac cgtatccagg aacagtcgtc ccgaaacacg gccgccattt
     3061 atcagcgttt tgtggagacc ggcgaacagc tatcgcagat gcggcgattg ctcttcgccg
     3121 cctccctcac catcagggat tatctattct gcaaggaagc ggcacggtcg gcggccttcc
     3181 gcacggagtt gcgagagctg gccgctcaga cccgccaggc cctgcagaca ctcgagcact
     3241 cgatcggaca ccggcagacg ggcgagttac gcgaaatgat caacggcttc ctgggcacca
     3301 tcgagctggc tatcccctgg ccggatgaac aacgcgccac cgccggtttc gctttcatcc
     3361 aatcggagat cgcgccgcgc cgcggtgcag cgagcggcgt gctgcgggaa ctcatgagtg
     3421 tgaaccagca gctcctcgaa acaagccggc aacagttcgc agcctcctgg cgcgctgccg
     3481 ccgcccgcct gtctatcatg ctcgtgcttt gcatcgtcat tggcgtggtt gtggcccacg
     3541 ccagcgttgg ccacgtctcc cggctggaag cacagagcat ccgccggttc gaggaggtga
     3601 tgcaggcgaa agaagaactc gcacgtctct ccgcgcgcct gctgcaggtt caagaggatg
     3661 agaggcggcg gctttcccgc gagcttcacg atgagatcgg ccagaccctt accgcgctac
     3721 ggatcgaaat ttcccatgcc cgggacgcgt ggctggccgg ctcgccttcg ctctccgcca
     3781 ggttgaacga ggcgcatgcc ctggcggaac gcaccgtcaa cacggtacgc gacatttcgt
     3841 tgctgctccg gccttcactg ctcgacgacc tgggactgga acccgccctt cagcaacagg
     3901 tggaggattt tagccgccgc tccgggattc gctgcacatt ggaacagacc aggctggacg
     3961 acaccctgcc ggattccgtc aaaacctgcg tgtaccgcgc cgtccaggag gcgctgaaca
     4021 actgcgaaaa gcattcgggc gcctcgtggg ttcgtataca ggcgcttcag gacgggcctt
     4081 tgttgtccgt cgagatcgag gacaacgggg tggggtttga agtgaaccgg cacggagcgg
     4141 cgctcggctc cgggctcggg ttgatcggca tgcgggagcg tgtggccgcg ctgggcggca
     4201 gactaaccct cgtatccgca gccggcgaag gaacgaagct gtcgctgacg gttccgctac
     4261 aaggtcaggc gggctccagg ataccggtta gggcggaggc ggccgaaaac tgaatgccca
     4321 caaatatcct tctggccgac gaccattgca tcctgcggca ggggttgcgc aggattcttg
     4381 aaagctatcc ggatttcacc gtcgtggcgg aagccgggtc cggacgccag gcggtggaac
     4441 ttgcccgcga gcatcagccg gacgtcgcca tcgtcgatat cgcgatgggc gagctgaacg
     4501 gaatcgacgc caccgcgcag atcatccggt actctccgcg aacggcggtt atcatcctca
     4561 gcatgcacgg cgacgaacgc tatatcgtcc gcgctctgaa cgcgggggct aagggatatc
     4621 tgctcaaaga ttccgtggag caagacctgg tgcaggcgat tcaatccgtc tgcgcggacc
     4681 ggccgttctt cagcccggct atcagacagg tgctggcgga acggcaggcg tccgcgtccg
     4741 tcccgggtca cgttgaagat cgttacgatc tgctgaccgg gcgcgaacgg cagatctatc
     4801 aactacttgc cgaaggcagg agcaataagg atattgcttc cctgctggta ttgagcctgc
     4861 acaccgtgga aacgcatcgt acccgcatca tggagaaaat gaatgtgcac agcgcggcgg
     4921 aactggtgct cagcgccgtc cgccgcggaa tcgtgcgata gccggcggct aggagagcta
     4981 atcaggtatc agtacgggct gtttgaccgg tcccctggct ccccgctatt ataagagtga
     5041 tttagcacgt taggaggcat gtctctcgaa tgggtcctct gggctggcag gaaactgtat
     5101 ttatctttgt cctt
//
LOCUS       DASTMK010000526         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_474252, whole genome shotgun sequence.
ACCESSION   DASTMK010000526 DASTMK010000000
VERSION     DASTMK010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474252"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFQ79_21800"
     CDS             <1..277
                     /locus_tag="VFQ79_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142371.1"
                     /translation="ANFSYKTLMVAGMHFMDHYNYDIERVKRCVIHYAAPNGLIYPFC
                     AYNGGPTYRERIELAYSVAIEDQPQMKVLRTGRNGQRHSPLVTVTLS"
     gene            386..1261
                     /locus_tag="VFQ79_21805"
     CDS             386..1261
                     /locus_tag="VFQ79_21805"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0142372.1"
                     /translation="MTSETLEKLLVEELRDLYDAEKQLVKATAKMAKAAKTPELKQAF
                     QHHNEQTRGQVGRLEQMFEVLGKKARGKPCKGMKGLVEEGQEVMEEELEDEAYDLAII
                     GAGQRVEHYEIAAYNGARSMAAQLGMKEVVTLLQESLDEENQTEKLLTQLSKALLKQT
                     GRSRPVEAGESTKATSGAGAKKRASRKASRQGNLTHFTTDHDEIRQWAEERGGKPACV
                     RGTGGKGDVGMLRLDFPGFSGQGSLEPIDWDTWFRKFDDENLALLYQDKTSRGEKSNF
                     NKLVSRETASSGYRA"
     gene            1516..2094
                     /locus_tag="VFQ79_21810"
     CDS             1516..2094
                     /locus_tag="VFQ79_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142373.1"
                     /translation="MHRQVDEVRIVVDDTQPSDKDQATFDLRKGYAALLIKTLFPARF
                     RYRKVGGREPAETPHGDLFYQGRSARHFHRHVKDFWIRDFSEGVIPNHKSFNWPPQRP
                     HEAAEDAYVAVYGPGYGRWRLEIEPDAPAQTDYFLNVLKPSVNPDERLPPVTKAETEA
                     TFGASITKDGRKYVVTFSKDSLESPRLEITAR"
     gene            2121..2264
                     /locus_tag="VFQ79_21815"
     CDS             2121..2264
                     /locus_tag="VFQ79_21815"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0142374.1"
                     /translation="MRTGEAFSGVRVLNKAVDVLEILRSNPGGMTLASLSAETALPKH
                     SYS"
     gene            2545..3483
                     /locus_tag="VFQ79_21820"
     CDS             2545..3483
                     /locus_tag="VFQ79_21820"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU0142375.1"
                     /translation="MPWPASPVSRGSPETAGRPAFPPSLVIQVKALACELPHRLGLPL
                     SRFSVAEIRQEVLAQGLIADISGTTIWRWLSQDALCPWRHRTWIFPRDPRFVQKAGPI
                     LDLYHGLWEGQSLGSGDFVLSADEKTSIQARRRIHASMPPAPGRPARIEHEYERRGAW
                     AYLAAWDVHRAKIFGRCEASTGIVPFDRLLEDVMGQEPYRSARRVFWIFDNGSSHRGQ
                     KADQRLQSRWPSIIPVHTPVHASWLNQVEIYFSIIQRKVLTPGDFSNPTDLEMALLAF
                     QQRYEKSAVPFRWTFTRADLADLMQRLKASALPAAA"
     gene            3683..4177
                     /locus_tag="VFQ79_21825"
     CDS             3683..4177
                     /locus_tag="VFQ79_21825"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0142376.1"
                     /translation="MEKLVAFCKETLNLGVLDGGEVVVIETMESPQAVRMSSKIGNRR
                     YPHSTALGKVLLAGLPERDVLRIVQARKMPRFTPATITSERALLIELERVRQQGYAFD
                     NCENEPDGRCVAAPIFGAGRNVVAALSISGPLPRMTMTCLRSFVKRLCTASRSVSAAI
                     GGDR"
     gene            complement(4253..5221)
                     /locus_tag="VFQ79_21830"
     CDS             complement(4253..5221)
                     /locus_tag="VFQ79_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEU0142377.1"
                     /translation="MKAIQVTEVGGPERMQLAEVPMPKPGPGQAVVKIAASGINYIDV
                     YYRTGLYKAELPVILGMEAAGIVEATGADVTEVSVGDKVAYAMDRGSYAEYAVVPAWH
                     LAKVPDGLELELAAAVMLQGMTAHYLTHSTFPLKEGDTALIHAAAGGAGLMTVQMAKM
                     RGARVIGTCSTEAKASIAKNAGADDVILYTEQDFESEVRRLTNGRGVDVVYDSVGKET
                     FLKSLSCLRPRGMMVSFGNASGAVPPVEPLLLSQKGSLFLTRPNLAAYTATREELMWR
                     AGDVLSWTAQKKLAVHIHQVYTLDEAPRAHQELEARKTSGKLLLKP"
     gene            complement(5218..5766)
                     /locus_tag="VFQ79_21835"
     CDS             complement(5218..5766)
                     /locus_tag="VFQ79_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142378.1"
                     /translation="MRYFFSRHIPPLRRMLIVESGSRSLVEEFLSKLQTSHGNSLRVD
                     LVTCFGGVPRGFSDDVGKVYRVSDYRDSPARKRLFRKLRSNRYSGVVLLCTGEPIMTK
                     WKWVLAAKVPSKICILNENGDYFWLDWSNWRIIRHFVSYRAGLSGTGAVVTPFRLLVF
                     PLTLAYLLLFAIVVHLRRKVHA"
     gene            complement(5802..>6129)
                     /locus_tag="VFQ79_21840"
     CDS             complement(5802..>6129)
                     /locus_tag="VFQ79_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142379.1"
                     /translation="AGQSLYLLERDRRDGDLDVSVLHGEVLTDLLGIDEAAVREERNI
                     HYVRGLDAAVAEIRNGTAQAAFLLEPATIEQVASVSFAGGVMPQKSTDFYPKLLSGLT
                     IYRLEE"
BASE COUNT         1304 a         1861 c         1762 g         1202 t
ORIGIN      
        1 agcgaatttt tcctataaga ctcttatggt tgccggcatg cacttcatgg atcactataa
       61 ctatgacatc gagcgtgtaa agcggtgcgt gatccactat gcggcaccca acggccttat
      121 ctacccattc tgtgcctaca atggcggtcc cacttatcga gaaagaatcg agctcgccta
      181 ctccgtggcc atcgaggacc agccgcagat gaaggtcctg cgcaccggaa gaaacggcca
      241 gcgacactcg ccgcttgtaa cggtgactct ttcctgaccc gcgcgcaagc cctacagcca
      301 gcttctccga aacatttcta cgccttacgg gtcctacaga tccgagggag tgggagaact
      361 tcgttcaaag agaggctgcg tacgaatgac atccgaaacg ctggaaaaat tgctggttga
      421 agaactaagg gatctttatg atgccgagaa gcaattggtc aaggccaccg cgaagatggc
      481 gaaggccgct aaaacccctg aattgaagca ggcatttcaa catcacaacg agcagacacg
      541 cgggcaggta gggcgcctgg aacagatgtt cgaagtcctg ggcaaaaagg cacgaggcaa
      601 gccttgtaaa ggaatgaaag gccttgtgga agagggccag gaggtgatgg aagaggaact
      661 ggaggacgag gcgtacgatc ttgcgatcat cggtgcgggt cagagagtcg agcactacga
      721 gattgcagct tacaacggcg cgcgaagtat ggccgcacaa ctggggatga aggaggttgt
      781 cacgcttctt caggaaagtt tggacgaaga gaaccaaacg gagaagctgt tgacacaact
      841 ctccaaagca cttctcaaac agaccggccg ctcacggccc gttgaggcgg gcgaatcaac
      901 gaaagcaacc agcggcgccg gtgcaaagaa acgtgcgagc cgcaaagcat cgcgacaggg
      961 caatttgaca cacttcacta ccgatcacga tgagatccgg cagtgggctg aagagcgcgg
     1021 cggaaagccc gcttgcgtca ggggtaccgg tggcaagggc gacgtcggga tgctacggtt
     1081 ggattttcca ggcttcagcg ggcaggggtc gctcgagccc atcgattggg acacctggtt
     1141 ccgaaagttc gacgatgaaa acctggctct gctctatcag gacaaaacga gccgcggcga
     1201 aaagagcaac ttcaacaaac tggtcagccg cgaaacggcg tccagcggct atcgggcctg
     1261 aaaaggctcg tccgcgctcg cggcggcaag agcgccgctg aacgctggag catcagttcg
     1321 ttcctcgcca gaccggatta ataaaggctc cgttcgcggc gatatcccaa gccgcgaaac
     1381 gggccacttc caagccttgg cctcaactgt ccaattaaaa gtggactcgc caaacgggcg
     1441 catgttgtcg aggggaaaga tcttccgctg cgttgccgtg ccgtcactcc acacgacttc
     1501 cgcaaagcgc agtccgtgca tcggcaagtg gatgaagtca ggatcgtcgt cgatgacacg
     1561 cagccctccg ataaagacca ggcaacattc gatttgcgga aaggctatgc ggcactgctg
     1621 atcaagaccc tgttccctgc ccggttccgc taccgcaagg ttggcggccg tgagccggcg
     1681 gagacaccac acggcgacct gttctatcag ggacgcagcg cacgtcattt tcaccgccac
     1741 gtgaaggatt tctggatcag ggatttcagc gagggcgtga tcccgaacca taaatcgttc
     1801 aactggccgc cgcagcggcc gcacgaggcg gccgaggacg cttacgtggc tgtgtacggg
     1861 ccggggtatg gccgctggcg gctggagatc gagccggatg cgccagccca aacggactac
     1921 tttctgaacg tcctgaaacc ctccgtgaat ccggacgaga gattgcctcc cgtcacgaaa
     1981 gcagaaacgg aagccacttt tggcgcctcc attacgaaag acggccgcaa gtatgttgtg
     2041 accttcagca aggactcttt ggaatcgccc cgattggaaa tcacggcccg gtaacgtgag
     2101 aattcttata atgcttgctt atgagaacgg gtgaggcatt ctccggtgtg cgcgtgctca
     2161 acaaggcggt ggatgtactg gagatacttc gttccaatcc cggtgggatg acgctcgcca
     2221 gtctgtcggc cgagaccgca ctgcctaagc actcctattc gtaaatacgg tgacgtatta
     2281 ttttgtcgca ttcggtgacg taatctgata agcttttctt tggcgaactc cgcgcgctaa
     2341 tgccaaaaca aagcccgtat gttgtgcatc tcacggccga ggaacggcgg gagttggagg
     2401 ggatggcgcg tcggtatacg tcaccgtatt gtgacgttgt tcgcgccagg atcattctca
     2461 tggcggccga agggctttcc aatgacctga tcgctgcgcg cctcaatacg ccgcggcaaa
     2521 tcgtcagcaa gtggcggaag cggtttgcct tggcccgcct ccccggtctc gaggggcagc
     2581 ccagagacgg ccggaaggcc cgcttttccc cccagtctcg tcatccaggt caaagctctc
     2641 gcctgtgagt tgccccaccg cctcggcttg cctttgtccc gcttttcggt cgcggaaatc
     2701 cgtcaggaag tgctcgccca agggttgatc gccgacatca gcggcaccac catttggcgc
     2761 tggctcagtc aggacgctct ctgcccttgg cgccatcgca cttggatctt cccgcgcgac
     2821 cctcgcttcg ttcagaaggc cgggccgatc ttggacctgt accacggcct ctgggaaggt
     2881 cagtcgctcg gctccggtga tttcgtgctc tccgccgacg agaagaccag catccaggcc
     2941 cgccgccgca tccatgcctc aatgccgccg gctcccggcc gccccgcacg tatcgaacac
     3001 gagtacgagc ggcgcggcgc ttgggcatac ttggccgcct gggatgtcca tcgcgccaaa
     3061 atcttcggcc gctgtgaagc cagcacagga atcgtaccgt tcgatcggtt gctggaagac
     3121 gtaatgggtc aagaacccta ccgttcagcc cgtcgcgtgt tctggatctt cgacaatggt
     3181 tcctctcacc gcggacaaaa ggccgatcag cgcttgcagt cccgctggcc ctctatcatc
     3241 ccggtccaca ccccggttca cgccagttgg ctgaaccagg tcgagatcta cttctccatc
     3301 atccagcgga aagtgctcac ccccggcgac ttctccaatc ccactgacct ggagatggct
     3361 ctcctggctt tccagcaacg ctacgagaaa tccgccgtcc catttcgctg gacgttcacg
     3421 cgggctgacc tggccgacct catgcaacgc ctcaaagcca gcgccttgcc cgctgccgcc
     3481 tgaggcccac agaatacgtc accgtaattg cgaattgggg tacttagcca acggtctatc
     3541 ggatcgtggc cacactcgag tcgtgccggt atctgagccg cacctcggac ggcagctatc
     3601 gcctgtcgaa gaagatgttc ggactccgag tagacacggc cactcaggag caactgctct
     3661 gctccgcgcc gcacggccgg tcatggagaa actcgtcgct ttctgcaagg aaacgctgaa
     3721 tctcggagtg ctggatggcg gtgaggtggt ggtgatcgaa actatggaaa gcccccaggc
     3781 tgtgcgcatg tcgtcgaaaa tcggcaaccg tcgctatccc cactcaaccg ctctcgggaa
     3841 agtactgctg gcgggcttgc ccgagcgcga tgtattgcgc atcgtacaag cccggaagat
     3901 gccgcggttc acacccgcca cgatcaccag cgaacgtgcg ctcctgatcg aattggagcg
     3961 cgtgcgccag cagggctatg cattcgacaa ttgcgaaaac gagccggatg gccgctgcgt
     4021 ggcagcaccg atcttcggcg ccggccggaa cgttgtggct gccctcagta tctccggccc
     4081 tttgccacgc atgacgatga cgtgcctgcg ctctttcgtg aaacgattgt gcactgccag
     4141 ccgatcggtc tccgccgcca tcggcggcga ccgctgagta ctcctattcg gacatatccg
     4201 cttgcttgct cgcgcggctc ggtttcggaa gccgtgaccg tgagggagcg gtttacggtt
     4261 tcaacagcag cttcccgctg gtcttgcggg cttccagttc ctggtgtgct cgcggagcct
     4321 cgtccagcgt atagacctgg tgaatgtgca cggccagctt cttctgcgcg gtccagctca
     4381 gcacatcccc tgcccgccac atcaattctt cccgcgtggc tgtataagcg gccagatttg
     4441 gccgggtgag aaacagcgaa cccttttgac ttagtagaag cggctcgacg ggtggaacgg
     4501 ctccgctggc atttccaaag ctcaccatca tgccgcgggg gcgcaggcag ctcaggcttt
     4561 tgaggaatgt ctctttgcct acggaatcgt acaccacgtc cactccgcgg ccgttcgtaa
     4621 ggcgccggac ttccgattcg aaatcctgct cggtgtacag aatcacgtcg tcggcgccgg
     4681 cgttcttcgc gatcgaagct ttggcttcgg tggaacacgt gccgatgacc cgcgcgccgc
     4741 gcatcttcgc catctgcacc gtcatgaggc ccgcgccgcc ggcggcggca tgaataagcg
     4801 ccgtatcgcc ttccttcagc ggaaaagtgg aatgagttag ataatgggcg gtcattcctt
     4861 gcagcatgac ggcggctgcc agctcgagct ccagtccgtc gggaactttc gccaggtgcc
     4921 aggccggtac cacggcgtac tcggcgtacg agccgcgatc catggcataa gcgactttat
     4981 cgcccacgct gacctcggtg acatccgctc ccgtggcttc cacaattccg gcggcctcca
     5041 ttccaagaat caccgggagc tctgccttgt agagtcctgt gcggtagtac acatcgatgt
     5101 agttaatccc ggacgctgca attttcacta cggcctgtcc cgggccgggc tttggcatgg
     5161 gtacttccgc gagctgcata cgctcgggcc cgccaacttc cgttacttga atcgctttca
     5221 tgcgtgcacc tttcgtcgga gatggacaac gattgcgaag agcagcaggt acgccagcgt
     5281 aaggggaaac accagcagcc ggaacggcgt caccaccgcg ccggtgccgg acagcccggc
     5341 gcgataagac acaaagtggc gaatgatgcg ccagttggac caatcgagcc agaagtaatc
     5401 gccgttctca ttcaggatgc agatcttgct cggcaccttg gcggcaagaa cccatttcca
     5461 tttcgtcata atgggctcgc cggtacagag aaggacaaca cccgaatagc ggttggaacg
     5521 cagcttccgg aagagccgtt tgcgtgccgg agaatcacga tagtccgata cgcggtacac
     5581 cttccctaca tcgtcgctga aaccacgcgg cacaccgcca aaacaggtaa ccaggtccac
     5641 gcgcagggaa ttgccgtgcg aggtctgaag cttactcaga aactcctcta ccaggctgcg
     5701 tgagccgctc tccacgatca gcatccgcct cagaggcgga atgtgtctcg agaaaaaata
     5761 gcgcatcccg aagtaaccac tataccagcg gcctcggaag gttactcctc gagcctgtat
     5821 atggttagcc ccgacagcag tttcggataa aaatccgtcg atttctgtgg cattacgccg
     5881 cccgcgaaag ataccgatgc aacctgttcg atagtcgccg gctccagaag gaaggccgcc
     5941 tgcgccgtgc cgtttctgat ctccgcaacc gccgcatcca acccacgcac gtagtggatg
     6001 ttcctctctt cacgcaccgc ggcttcatcg atgcccagca ggtccgtgag cacttctccg
     6061 tgaagaacgg agacatcgag atcgccatcc cggcgatctc gttcgaggag atacaacgat
     6121 tggccggca
//
LOCUS       DASTMK010000527         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_474277, whole genome shotgun sequence.
ACCESSION   DASTMK010000527 DASTMK010000000
VERSION     DASTMK010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474277"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /gene="polA"
                     /locus_tag="VFQ79_21845"
     CDS             <1..2206
                     /gene="polA"
                     /locus_tag="VFQ79_21845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU0142380.1"
                     /translation="SCRALDSGVDVVIVSSDKDMMQLVNERVSMFNPVKEDAFYDPAG
                     VEQFMGVPPGKVADLLALRGDAIDNIPGAPGIGDKGARDLILRFGSAEQAIEHAEEVE
                     RKMYRESLRNYRDQILLSKRLAAIDTNVPIEFNLAAVAATAPDTDALKPLYRELEFFS
                     LLRELSAGDGLESSDFRQLDSEEAVRAYLDAIPEGAPLAIALKGGGEGELDLAMLGLS
                     SKAGEGRVVTAPLIAAAREALENEGRPKIGHDIKSLMLALERFGVSPCGFTDDIMIYG
                     FLLSADPSGCSPETLAEKYLARTLRGGAEHAAEAAFVLAAELRPQIESNGLRDLYETI
                     ELPLAPVLARMARTGIRIAPDQLLKLSESMDRELQRLTAEIHALAGKPFNINSPQQLG
                     KVLFEELGLPAPVKYGKGKTISTAADILENLACDFEICSKVLEYRQLAKLKGTYVDAL
                     PVLVDPETGRLHTTLNQAGAATGRLSSSNPNLQNIPIRTELGREIRTAFVPAQGWKLV
                     VADYSQIELRLLAHMSGDAVLLDAFRRGEDIHTRTAAEVFGVPPLMVDKELRRRAKAV
                     NFGIVYGQTPFGLAKELGIERKEAEQYIRNYFERYSGVRKFIDNTIAEVRQSGVSRTL
                     LGRMRPIPDMNSRNPNARGFAERTAVNTPLQGTAADLIKLAMIRIDRVLSERKLQARM
                     LLQVHDELLFEAPPEEAPGVAKMVKQEMEGVYKLDVPLLVDTGIGDNWRDAK"
     gene            2203..>3014
                     /locus_tag="VFQ79_21850"
     CDS             2203..>3014
                     /locus_tag="VFQ79_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142381.1"
                     /translation="MNGGAAKPPLSYSIPSFSRDGLDDCATVLQRLRGLGFRHVTFTP
                     AFQVLADAPLNIDLGRTPDYAVIDAAVALAHDMGFGVRMEPHLDYASTFSGGPYRWRR
                     DLVFDPSHESTGYFDKVIAPLAAALAPGDELTLGSELDLSLTVFSQGWERMRTMGPQI
                     LRCGHKVNHDFFDGRVPDAVFDYLRALEYVSFSFYPRIGWRRGWTPNDISAAFRQKAK
                     RLVEMLRARIAPSSTMTIGEFGLGSADVNSPWKVAAETLAGPEPLQARRDYYL"
BASE COUNT          592 a          900 c          948 g          574 t
ORIGIN      
        1 ctcctgccgt gctttggatt ccggtgtgga cgtggtgatt gtctccagcg acaaggacat
       61 gatgcaactc gtcaacgaac gcgtttccat gttcaacccg gtaaaggaag atgcctttta
      121 cgatcccgcg ggggtggaac agtttatggg cgttccaccg gggaaggttg ccgacctgct
      181 cgcattgagg ggcgatgcca tcgacaacat tccgggagcg cccggcatcg gcgacaaagg
      241 cgcccgcgac ctgatcttgc gtttcggctc ggcagaacag gcaattgagc acgcggagga
      301 agtcgagcgg aaaatgtacc gggagagcct gaggaattac cgcgatcaaa ttctgctcag
      361 caaacggttg gctgccatcg acacgaacgt tccgatcgaa ttcaacctcg ccgccgttgc
      421 ggctaccgcc ccggacacgg atgcgctcaa gccgttgtac cgcgaactgg aatttttcag
      481 cctgctcagg gaactttctg cgggtgatgg tctcgagtcc agcgactttc gccaactgga
      541 ttccgaagag gcggttcgcg cctacctgga tgcgattccg gaaggtgcgc cccttgccat
      601 cgccttgaaa ggcgggggcg agggggaact cgatctggcg atgctgggtc tttcctcgaa
      661 agcgggtgaa ggccgggtgg taacggcgcc tctgatcgca gcggcgcgcg aagcattgga
      721 gaatgagggc cgccctaaga tcggccacga catcaaaagc ctgatgctgg cactcgaacg
      781 cttcggggtc agcccgtgcg gctttaccga tgacatcatg atctatggtt tcctgctctc
      841 cgcggaccct tccggatgtt cgcccgagac gctggcggaa aagtatctcg cccgcacgct
      901 gcggggtgga gccgaacacg ccgccgaagc cgcgttcgtg ctggcggcgg agttgcggcc
      961 tcaaatcgag agcaacgggc tgcgggacct ctacgaaacc atcgaattgc cgcttgcccc
     1021 cgtgctggcg cgcatggcga gaaccggtat ccgcatcgcc ccggatcagt tactgaagct
     1081 ttccgagtcg atggaccgtg aactccaacg gctcacggcg gagatacacg cactggccgg
     1141 caagcctttc aacatcaact cgccgcaaca actcggcaag gtgctgttcg aagaactggg
     1201 attgcccgca ccggtgaaat acgggaaggg caagaccatt tctaccgccg ccgatattct
     1261 ggagaacctc gcctgcgatt ttgagatctg ctccaaggtt ctcgaataca ggcagttggc
     1321 caagctgaag ggcacttacg tagacgcgct tccggtgctg gtggatccgg aaacgggccg
     1381 gctgcacaca acgctcaacc aggcgggcgc ggccaccgga cgcctgtcct cctccaatcc
     1441 gaatcttcag aacataccca tccggacgga gctcgggcgt gagattcgca cggcgtttgt
     1501 ccctgcgcag ggctggaaac tggtggtggc ggactactcg cagattgagc ttcgcctgct
     1561 tgcacacatg tccggcgatg cggtgctgct cgatgccttc cggcgcgggg aggacattca
     1621 cacacgaacc gcagcagagg tcttcggtgt tccgccgctc atggtggata aagaactgcg
     1681 gcggcgcgcg aaagcggtaa acttcggaat cgtgtacggg caaacgccgt tcgggctcgc
     1741 gaaagaactc ggcatcgaac gcaaggaggc cgagcagtac atccggaact actttgaacg
     1801 ctactccggg gtgaggaagt tcatcgacaa taccatcgcg gaagtgcgcc aatcgggagt
     1861 ttcacgcacg cttctggggc gcatgaggcc catccccgac atgaacagca gaaatcccaa
     1921 tgcgagggga ttcgccgaga ggacggctgt gaacacgccg ctgcagggca ccgcggccga
     1981 tctcatcaag ctcgccatga tccggatcga tcgcgtgttg agcgaacgca aacttcaggc
     2041 gcggatgctg cttcaggtgc acgacgagct gttgttcgaa gccccgcccg aagaagcgcc
     2101 gggcgtggcg aagatggtga aacaggagat ggaaggcgtc tacaaactgg atgtgccgct
     2161 gctggtggat acgggcatcg gagacaactg gcgcgacgcc aaatgaacgg aggcgcggcg
     2221 aagcctccgc tctcctattc cattccgtct ttctcgcggg atgggcttga cgactgtgca
     2281 acggtgctcc agcggttgcg tgggctcggt ttccgccacg tcacgttcac acccgccttt
     2341 caggtgctcg cagatgcgcc gctcaatatc gacctgggcc gtacgccgga ttacgccgtg
     2401 atcgatgctg ctgtcgcgct cgcgcacgac atgggatttg gcgtccggat ggagccgcac
     2461 ctcgattatg cgtccacttt ctccggcggc ccctaccggt ggaggcgcga cctggtgttc
     2521 gacccttcgc acgaatccac cggctatttt gataaggtga tcgcaccact ggccgccgcg
     2581 ctggctccgg gcgacgaact gacgttggga tcggaactcg acctttcctt gacggtgttc
     2641 tcgcagggct gggagcggat gcgcacaatg ggccctcaga ttttgcggtg cggacacaag
     2701 gtcaatcacg atttcttcga cggccgcgtg ccggacgcgg tgttcgatta tcttcgcgct
     2761 ctcgaatacg tgtcgttcag cttttaccca cgcatcggat ggcgccgcgg ctggacgccc
     2821 aacgatatct ccgccgcgtt ccggcaaaag gcgaagcggc tggtagagat gctgcgggcg
     2881 cgcatcgcgc catcgtccac gatgaccatc ggcgagttcg ggctcggctc cgctgacgtg
     2941 aacagtccgt ggaaggttgc ggctgaaacc ttggccggac cggagccgct ccaggcacgc
     3001 cgcgactact atct
//
LOCUS       DASTMK010000528         5979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_29134, whole genome shotgun sequence.
ACCESSION   DASTMK010000528 DASTMK010000000
VERSION     DASTMK010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29134"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFQ79_21855"
     CDS             <1..732
                     /locus_tag="VFQ79_21855"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0142382.1"
                     /translation="ARQRASTGKAFEAGVAPSEVWEPGPYVRTLPKLFEHLRKTVGEE
                     IELLHDVHERVTPSQAIVLAKAVEPYRLFYFEDPVRPEHLDTFRIIRQQTTTPLAMGE
                     IYTGQWDGLYLITEHLIDYVRHDLAHCGGITTGKKIAAMCEPYGILTAWHGPGNISPV
                     THMANAHVSLSVPNFGIQEFAVGWGERVREVFSAMPAYSSGSITIQDGAGLGIDVNEA
                     SAKKYPYLRRLRPTIRREDGTPWPY"
     gene            796..1311
                     /locus_tag="VFQ79_21860"
     CDS             796..1311
                     /locus_tag="VFQ79_21860"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEU0142383.1"
                     /translation="MATFVERVIGAARLNAGIYEEVETDVSASGQAMGVVLLSSIGAG
                     IGAFGTLGLRGLLTGIVGSFLGWFLWAWLTYFVGTRILPSPETRADWMQLLRTTGFAT
                     APGILGVLGFFPVFRGFIFFVTSLWSLIAFVVAVRQALDYTSTLRALCVCAIGWIIYV
                     AMISLLLGTWG"
     gene            complement(1315..3360)
                     /locus_tag="VFQ79_21865"
     CDS             complement(1315..3360)
                     /locus_tag="VFQ79_21865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU0142384.1"
                     /translation="MNPADNPLLEIQFHVPFDRIKAEHVEPAARQLLASAREELERIA
                     SGPDPRTYENTLVELDEFTRNLDYAMNVVRHLESVATYPELRAAYNTVHPLTSEFYSN
                     IPLHEGLWNSIKKYAETGEARGLKGTRNRYLTKTLHEFRRHGADLDAAGKTRLAEIEV
                     ELAKLTTKFAENVLDTTNAFELVITDEAKLAGLPPTALEAARQNAASKGIEGWRFTLQ
                     APSYVPVMTYLDDRGIREHMYRAYVMRGTAEGFDNRPIIERLLELRREEAALIGFRDF
                     ADFVLEDRMAHTGDRAESFLESLKDKTVEHFRRENDDLAAFRRSLEGEEAPGLEPWDI
                     GYYAEKQRAALYDFDEEALRPYFPLPRVVEGMFEIFGRLLGIRVTEKSGVPAWDPGVR
                     YFEIRDAASGELLGGFYEDFYPRENKRGGAWMDALITGVQTPKGLEPHLGLICGNLTP
                     PVGDAPALLTHREVETIFHEFGHLLHHCLSRVEMRGLAGTNVAWDFVELPSQIMENWC
                     WECESLDLFARHYQTGEPIPGDLFQKMRRARTFRAANGQMRQLSFGLLDFALHRRYSE
                     AEHGDVIAYSRAILQEFSPAPLPPEHAMIAGFTHLFASPVGYAAGYYSYKWAEVLDAD
                     AFTRFQNEGLFNPQTGAEYRTKILERGDSDDPMLLFRSFMGRDPDPNALLARLGLAG"
     gene            complement(3360..3977)
                     /locus_tag="VFQ79_21870"
     CDS             complement(3360..3977)
                     /locus_tag="VFQ79_21870"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0142385.1"
                     /translation="MIRLSVVLLAVCAACFGQVSKAEKKEGFRPLFDGKTLNGWDGDP
                     RLWSVRDGVIVGSTEGVELTDNTFLISRRTFGDFVLRTEVKLRNHNSGIQFRSEALPN
                     WVVRGLQADVAEGNWWGSIYDEKGKRGVIVNGWQGKAEKVVRPGDWNDYEIVCKGDLI
                     QLRVNGLLTAELRDSSKTSGILALQLHRGPAMQVEFRNIRLRELK"
     gene            complement(3988..4542)
                     /gene="rnhA"
                     /locus_tag="VFQ79_21875"
     CDS             complement(3988..4542)
                     /gene="rnhA"
                     /locus_tag="VFQ79_21875"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEU0142386.1"
                     /translation="MRRSIPRGKPARSSIWSIDSSSRNWRPFLPEAPFKKVQVITDGA
                     CLGNPGPGGWAAILRYKDKRKELWGAEAHTTNNRMELTAAVRGLAALKEGCEVEVVTD
                     SEYLKNGITSWILNWKRNGWKTKQKKPVVNQDLWMQLDEQNRRHKTTWVWTKGHASHE
                     DNNRADELAAMAAREQRTSAGGMR"
     gene            complement(4451..5467)
                     /gene="dusB"
                     /locus_tag="VFQ79_21880"
     CDS             complement(4451..5467)
                     /gene="dusB"
                     /locus_tag="VFQ79_21880"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU0142387.1"
                     /translation="MPQFPTHYRIGTVEVRPATVLAPMAGVTDTVFRRLIRGFGGCGL
                     LMTEFTSSHGVVAIDRRPNKLTVTRRYLAFEEEERPIAAQLFGSDPQVMYDAARICQD
                     LGFDAVDINFGCPVKKVVKCNGGSGLLRDLPLVEAILRSVRAAISIPLTMKFRAGWND
                     RELVAVRMARLAEDCGLQAIALHPRTREQGYSGKADWIRIAEVKDAVKIPVVGNGDIV
                     TPEDAVRMVAETNCDAVMIGRAASSNPWIFRQIAQYLETGSYDHPTEKHRYDIMRTYY
                     AMLTAHGESDAVGKMKQFATYFTHGVRNGARLRASIYTARETGEILDLVDRFFEQELE
                     AVPA"
     gene            5554..>5979
                     /locus_tag="VFQ79_21885"
     CDS             5554..>5979
                     /locus_tag="VFQ79_21885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0142388.1"
                     /translation="MQAHWDKVYSTKGPNEVSWYRPHLDRSIVLIERAVPDRSARIID
                     VGGGTSTLVDDLLQLGYFNITVLDISEAAIAVAQSRSRDASPGVRWLCADITNIEFAT
                     GSYDLWHDRAVFHFLTALDARLRYVRNVAKAVKPGGHVIV"
BASE COUNT         1108 a         1861 c         1755 g         1255 t
ORIGIN      
        1 gcccggcagc gcgcgtccac cgggaaagcc tttgaggccg gggttgcgcc atcggaagta
       61 tgggagccgg gaccatacgt gcgcacgctg ccgaagctct tcgagcacct tcgcaagacc
      121 gttggcgaag agatcgagct gctgcacgat gtgcatgaac gggtgacgcc cagccaggcc
      181 attgtactgg cgaaggcagt ggaaccctac cgtcttttct atttcgaaga cccggtccgg
      241 cccgagcacc tcgacacctt ccgcatcatc cgccagcaga cgaccacgcc gctcgccatg
      301 ggagagatct acaccgggca gtgggacggc ctgtatctga tcaccgaaca cctgatcgat
      361 tacgtgcgcc acgacctggc gcattgtggc ggtatcacca ccggtaaaaa gattgcggca
      421 atgtgcgagc catacggcat cctgacggcc tggcatggcc ccgggaacat ctcgccggtt
      481 acgcacatgg ctaatgcgca cgtaagcctg tccgttccca atttcggcat tcaggaattc
      541 gccgtgggct ggggagagcg ggtgcgcgaa gttttcagtg cgatgccggc ctactccagc
      601 ggttccatca caattcagga cggtgcgggt ttgggtatcg acgtaaacga agcgtccgca
      661 aaaaaatacc cgtacctgcg ccgcctgcgg cctacgattc gccgcgagga cggaacgccg
      721 tggccttact gatccgcgcc tcgttgccgg cccttccgcc gccccaatgc tataaccgga
      781 agggagaccg aatccatggc cacattcgtc gaacgcgtga tcggggccgc tagactcaat
      841 gccggcatct atgaggaggt cgagacggac gtctccgcgt ccgggcaagc gatgggagtg
      901 gtcctgttgt cgagtatcgg cgcggggatc ggggccttcg ggacactcgg cctgcgtggc
      961 ttgttaaccg gcattgtcgg ttcttttctc ggctggttcc tctgggcctg gctcacctac
     1021 ttcgtcggca cgcgaattct accgtcgccc gagacgcggg cggattggat gcagttgctc
     1081 cgcacgaccg gattcgccac cgcgccaggt attctcggag tactcggctt cttcccggtg
     1141 tttcgcggat tcattttctt cgttacgtct ttgtggagcc tgatcgcgtt tgttgtcgcg
     1201 gtgcgtcagg cgctcgacta taccagcacg ctgagggcat tgtgtgtgtg tgcgatcggt
     1261 tggatcattt atgtggcaat gatttcgctg ttgctgggca cgtggggctg aggctcagcc
     1321 ggccagtccc agcctcgcga gtagcgcgtt ggggtccgga tcgcggccca tgaaggaccg
     1381 gaacagcagc atggggtcgt cgctgtctcc ccgctccagg attttcgtcc ggtattcggc
     1441 gccggtctgg ggattgaaca ggccctcgtt ctggaagcgg gtgaaggcat cggcatccag
     1501 cacttctgcc catttgtagg aatagtagcc cgcggcgtaa ccgacggggc tggcaaacag
     1561 atgggtgaag cccgcgatca tcgcatgctc gggcggcagc ggagcgggcg agaactcctg
     1621 caagatggcc cggctgtagg cgatgacgtc gccatgctcc gcttccgaat accggcggtg
     1681 cagtgcgaag tccaaaagac cgaagctcag ttgccgcatc tggccgttcg ccgcccggaa
     1741 tgtgcgggcg cggcgcatct tctgaaacag atcgccgggg ataggctcgc cggtctggta
     1801 atggcgcgca aaaagatcga gggactcgca ctcccagcac cagttctcca taatctgcga
     1861 aggcagctcc acaaaatccc aggccacgtt tgttccggca agtccccgca tctccacgcg
     1921 gctcaggcaa tgatgcagca ggtgtccgaa ctcgtggaaa atggtctcca cttcgcgatg
     1981 ggtcagcaag gcgggtgcat cgccgacagg cggcgtcagg ttgccgcaga tcagacccag
     2041 gtgcggctcc agccctttgg gggtctggac gccggtaatc agagcatcca tccaggcgcc
     2101 gccgcgcttg ttttcgcgag gatagaaatc ctcgtaaaaa cccccgagaa gctcgcccga
     2161 agcggcgtca cgaatctcga aataacgcac tccggggtcc cacgccggaa cgccggattt
     2221 ttccgtcacc cggatgccga gaagccgccc gaaaatctcg aacatccctt ccactacgcg
     2281 aggcagcgga aaataagggc gtaaagcctc ttcatcgaag tcgtacagcg cggcgcgctg
     2341 cttttcggca tagtagccga tgtcccaagg ctccaggcca ggggcctcct ctccttcgag
     2401 cgaacgccgg aacgccgcca gatcgtcgtt ttcccgccgg aaatgctcga ccgttttgtc
     2461 cttcagggat tcgagaaagc tctccgcgcg gtcgccggtg tgcgccatcc ggtcttccag
     2521 cacgaaatcc gcaaagtccc gaaagccgat cagcgccgct tcctcgcgcc gcagttcgag
     2581 gaggcgctca ataatcggcc ggttatcgaa gccttcggcg gtgccgcgca tgacataggc
     2641 ccggtacatg tgctcgcgaa tgccgcggtc gtcgagatag gtcatcaccg ggacgtaact
     2701 gggcgcctgt agcgtgaatc gccagccttc tatgcccttc gaagccgcgt tctgcctggc
     2761 ggcctccaac gccgtcggcg gcaggcccgc gagcttcgct tcgtccgtga tgacaagctc
     2821 gaaggcgttg gttgtgtcga gaacattttc ggcgaatttt gtcgtcagct tcgccagttc
     2881 cacctcgatc tcggcaaggc gtgttttccc ggcggcatcc aggtcggcgc catggcgccg
     2941 gaactcgtgc agcgttttgg tcaggtatct gtttcgggta cctttgagcc cccgcgcctc
     3001 gccggtttca gcatactttt tgatgctgtt ccaaagtccc tcgtgaagcg gaatgttcga
     3061 atagaactcg ctcgtcagcg ggtgcaccgt gttataggcc gcccgcagct cagggtaggt
     3121 tgcgacggac tccaggtggc gcacgacgtt catcgcgtag tccaggttcc gggtgaactc
     3181 gtccagttca accagcgtgt tctcatacgt gcgcggatcg gggccggagg cgatcctctc
     3241 caattcctcg cgcgccgacg ccagcaattg ccgcgcggca ggctcgacat gttccgcctt
     3301 gatccggtca aacggaacgt gaaattgaat ttcgagaagt ggattatctg ccgggttcat
     3361 tacttcaact cgcgcaaacg gatgtttcga aactccacct gcatggcggg accgcgatga
     3421 agttgcagcg caaggatgcc gctcgtcttg gacgaatccc gcagctcggc cgtcaacagg
     3481 ccgtttacgc gaagttgaat gaggtcgcct ttgcagacga tttcataatc gttccagtcg
     3541 ccgggccgga ctactttctc cgccttgccc tgccacccgt tcacgatgac tccgcgtttg
     3601 cctttctcgt cgtagatgga gccccaccag ttcccttccg ccacgtcggc ctgtagcccg
     3661 cgcaccaccc agttcggcaa cgcttcacta cggaattgaa ttccgctgtt atgattgcga
     3721 agcttcactt ccgttcgcag cacgaaatcc ccaaacgtac ggcggctgat gagaaaggta
     3781 ttgtccgtga gttctacgcc ttccgtggat cccacgatta cgccgtcgcg aacgctccac
     3841 aaccgggggt cgccatccca tccattcagg gtcttgccgt cgaacagcgg gcggaaccct
     3901 tccttctttt cggctttgga cacttgaccg aagcaggcgg cacagaccgc cagcagcaca
     3961 acagacaatc ggatcatcga cggaatttta ccgcatccct ccggcgctcg ttctttgctc
     4021 gcgggctgcc atggcggcca gttcgtccgc gcggttgttg tcttcatgtg acgcatgacc
     4081 tttcgtccac acccaggtgg tcttgtgccg cctgttctgc tcatccagtt gcatccagag
     4141 atcctggttc accaccggct tcttctgctt cgtcttccaa ccgttccgct tccaattgag
     4201 gatccaggag gtaatgccgt tcttgaggta ttccgaatcg gtgaccacct ccacctcaca
     4261 gccttctttc aatgcagcca ggcctcgcac tgcggcggtc aattccatgc ggttgttggt
     4321 agtgtgcgct tcggcgcccc aaagttcctt cctcttgtct ttgtagcgaa ggatagcggc
     4381 ccagccgccg ggacccggat ttcccaggca cgctccgtcg gtgatgacct gtactttttt
     4441 gaagggggct tcaggcagga acggcctcca attcctgctc gaagaatcga tcgaccaaat
     4501 cgaggatctc gccggtttcc cgcgcggtat agatcgacgc acgcaggcgc gctccatttc
     4561 tcaccccgtg cgtgaagtac gtggcgaact gcttcatctt ccccaccgcg tcggactcgc
     4621 catgtgccgt gagcatcgcg tagtaggttc gcatgatgtc gtagcggtgc ttctcggttg
     4681 gatgatcgta gcttccggtc tccaggtact gtgcgatctg ccggaagatc cacggattgg
     4741 acgaagcggc gcgcccgatc atgacggcat cgcaattggt ttccgccacc atgcgaaccg
     4801 cgtcttccgg cgtgacaatg tcgccattgc cgacgacggg gatcttcacg gcatccttta
     4861 cttcggcgat gcggatccaa tccgccttgc cgctgtagcc ctgctcccgc gtgcgcggat
     4921 ggagcgcaat ggcctgaagc ccgcaatcct cggccaaccg ggccatgcgc accgccacca
     4981 gttcgcggtc gttccatccg gcgcgaaatt tcatggtgag cggaatggaa atcgccgcgc
     5041 gaacgctgcg cagaatggct tctaccagtg gcaggtcccg caacagacca gagcccccgt
     5101 tgcacttgac aaccttcttg accgggcacc cgaaattgat atccaccgcg tcgaaaccca
     5161 ggtcctggca gatgcgggcg gcatcgtaca tcacttgcgg gtccgagccg aacaactgcg
     5221 cggcaatcgg ccgctcctct tcttcgaagg caagatagcg cctggtgacg gtgagcttat
     5281 tcggcctgcg atcaatggca accactccgt gcgagctggt gaattcggtc atcagcagcc
     5341 cgcatccccc gaatccgcgg atgaggcgcc ggaataccgt atcggtaaca cccgccatgg
     5401 gcgcaagcac cgtggccggg cgcacttcga cagtaccgat ccggtaatga gtgggaaact
     5461 gcggcatgcg tacttattca ttttaaccgc agcgcggcgc ggtgggcgaa ttacataccc
     5521 gacacgggcc gcgaatcgct aagatgaagg atcatgcaag cgcattggga caaggtgtac
     5581 tccacgaaag gcccgaacga agtgagctgg tatcgcccgc atcttgaccg gtctatcgta
     5641 ctgatcgagc gcgccgtccc ggaccggtct gcccgaatca ttgatgtcgg cgggggaacc
     5701 tccacactcg tggacgacct gcttcagctc gggtatttca atatcaccgt gctcgacatc
     5761 tccgaagctg caatcgctgt agcacagagc cggtcgcggg atgcgtcgcc aggtgtcaga
     5821 tggctctgcg ccgacattac gaacatcgaa ttcgctaccg gatcttacga tctgtggcat
     5881 gatcgcgccg tctttcactt ccttacagca ctcgatgccc gtctccggta tgttcgtaat
     5941 gtcgcgaagg cggtaaagcc cggcggtcac gtcattgtg
//
LOCUS       DASTMK010000529         5803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_123379, whole genome shotgun sequence.
ACCESSION   DASTMK010000529 DASTMK010000000
VERSION     DASTMK010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123379"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..420)
                     /locus_tag="VFQ79_21890"
     CDS             complement(229..420)
                     /locus_tag="VFQ79_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142389.1"
                     /translation="MPPRMIVVAGPPGSGKSSIFPVSSFGISCFNADDRAAELNHGSY
                     VGIPSTSAMSSTASSKRSS"
     gene            complement(420..617)
                     /locus_tag="VFQ79_21895"
     CDS             complement(420..617)
                     /locus_tag="VFQ79_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142390.1"
                     /translation="MRMISLPRARNQKPKEDPHYPAAVKRAREAARRLQEAGIIDAAG
                     RRIRKDLPPDMQKGADRDFGG"
     gene            complement(761..1177)
                     /locus_tag="VFQ79_21900"
     CDS             complement(761..1177)
                     /locus_tag="VFQ79_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142391.1"
                     /translation="MGVVRAKIQTVEEIYAIVWRAVTGRRPIAAVYHGRRRLLCPHRL
                     GRNNAGQLRVLCYQYGGESQRGLEPPGSPSNWRCIVLEKLSRVEILEDNIWQTAPNHS
                     RPQTCVPNADVDAEDYPAEGMRTLRKRTKPGKSERA"
     gene            complement(1181..2041)
                     /locus_tag="VFQ79_21905"
     CDS             complement(1181..2041)
                     /locus_tag="VFQ79_21905"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0142392.1"
                     /translation="MSSEIVRFVERQDWLEPVEETVQQAVETAVESAGPAVANALYGT
                     WLGHPLHPVLTDIPLGAWTAAVVLDALDSRDGGDGLKRGADAAVALGLMGAVGSAITG
                     FTDWHKITGKPHRIGLVHGLLNTIGAGLFAASYICRQRHDRESGRRLAILGYAAAGMA
                     AHLGGHLVYREKIGVDHAPKEFPQDFTPVLDASELNEGELKRADAGGVPVVLLKRGDQ
                     IFALAETCSHLGGPLAEGQVEDGDCVRCPWHGSLFSMQDGSVREGPATHPQPCLEARI
                     NEGKVEVRGG"
     gene            complement(2081..4309)
                     /locus_tag="VFQ79_21910"
     CDS             complement(2081..4309)
                     /locus_tag="VFQ79_21910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0142393.1"
                     /translation="MGSRKHDGERSLTGLWPALSIILTVTAAWGACDFRPEVDPLLRE
                     ARLAFDDARLDRDQRLALLRAAAMAYPDDVVLHHEYQDHIANELSEPVRDLVIEEYRK
                     LASEHAGSVAARYLYARSLVGRRTRQAVELFEQLAGQHAEFPWPHLALAGAHTKGGRQ
                     HLEHFFRLCPGTLNEASYTALMEAGAPELRRAVAEAARKELSASKLPSRLADYHYLWE
                     LEIADAGPAERAALLKRIREDAARLRSLDTRRRNALLPALLETYEITGDRAGWEWARG
                     EIAKWLPHSFTAARLTLDEWRDSHLQPAINDSRETVAAYYRQLYRASAEWIRTWPMSP
                     EAWALRWKAARELPDLPDQEVEAVGSGTLTAVARNGSSRTLSPRVDIAATFLARNIAT
                     EKIPEIVGEAIREAEIRALTDIRPGWMSDDFRAAARRELDETRSHGRLVLVRFYMHSG
                     EPGKARQVLRDMKTDLVHDAADAQRYWAGRPDLTGALVELREFEEARAVLAEMKQWLA
                     GGTEERAAESGGRQADYWLSMARLAEAEGRKLDAAAYYRLESENRTGWEYFAERFARI
                     DKMLELWREMGGTEEGFRAWAGIREPAGGWQSASQPLIGFELRDLAGRSWSATDFRGK
                     VTLVNLWATWCGPCLVELPHLEKLYRQMEPGGEMQFVTFNVDEDAESLPAFLKRYGYT
                     FPVIPAREFIQDRMRVTMYPRTWIVDRDGTIRYEHAGFSSRPERWLNETLELLRRLAK
                     VN"
     gene            complement(4273..5544)
                     /locus_tag="VFQ79_21915"
     CDS             complement(4273..5544)
                     /locus_tag="VFQ79_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142394.1"
                     /translation="MSFQANDTIQIALIGAGGMGQGDARYATSLPGVKLVAACDIYDG
                     RLERMKEIWGNDLYTTRDFREVLNRKDVDAVIIGTPDHWHAPISILALNAGKDVYCEK
                     PMIHSVDEGKRVIEAQKKSGRMFQVGSQYVSSMIFDKARQLLAAGALGEVNMVEAWLD
                     RNTAVGAWQYSIPLDATPERIDWDRFLGNAPKRPFEPIRLFRWRNYNDYGTGVAGDLF
                     VHLLSGLHFATGSMGPNRVYATGGLRFWKDGRDAPDVMLATMDYPKTSAHPEFTFALR
                     VNFASGGSSEKFGFRFVGSEGVMTTTMSELTLSRRPRETEPGYTIGTFPKAVQEQFLK
                     EYRAKYPEQRPSADAMRPDREETFEPPRSHNAHLEHHKNFYAAVRSRKLFLEDAVFGM
                     RTAAPALLCNMSASRQRVYRWDPESMTAKEA"
     gene            complement(5549..>5803)
                     /locus_tag="VFQ79_21920"
     CDS             complement(5549..>5803)
                     /locus_tag="VFQ79_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142395.1"
                     /translation="KYPETAATADSMRPAAEETYLPPRGYSDSVDHFRNFFEAVRSRK
                     PVVEDAVFGFRAAGPALLSLVSYFEQKQCRWDPETMTVKG"
BASE COUNT         1077 a         1865 c         1691 g         1170 t
ORIGIN      
        1 gtagacttgg caactgccta gactgattct gaagggctta gcctattttt gatccttttc
       61 gtgacgaact tgggttaaca cgctctacgt gcattgcgaa atcgcgcaat gccagatagc
      121 gcatctccgt cacaaagccc accgctttcg ccagcgatgc ctgtttaaac gtcaccgcac
      181 tgcgtagagt cgtttcgatg gcaaaactcg ttcggctctc tatgcaggct aggacgaacg
      241 cttcgaactc gcggttgacg acatggcgga tgtgcttgga atgccgacgt acgagccgtg
      301 gttgagttcg gcggcgcgat catcggcgtt aaaacaggag atgccgaagc tcgatacggg
      361 gaaaatcgaa gacttaccgc tgccaggcgg cccggcgacc acgatcattc tgggaggcat
      421 caaccgccaa agtcacgatc cgcgcctttc tgcatgtcgg gaggcagatc cttgcggatc
      481 cggcgccctg cggcgtcgat aattccagcc tcctgaagcc ggcgagccgc ttccctggcg
      541 cgcttcaccg ccgccggata gtgcggatct tccttcggct tctgattcct cgctctcgga
      601 agcgagatca tcctcatagc ttttatttta cccggaatcg cttttcttcc gtgcgtactt
      661 ggtctcctgc agccgtacag ggtggccttt taccagtcag gctccggagc aggacgaatc
      721 tggagcgacc tattttaatg ggccatctaa tgtggcgtcc tcacgcacgc tcactttttc
      781 ccggcttcgt gcgtttgcgt agtgtgcgca tgccctcggc cggatagtct tcggcatcca
      841 catcggcgtt tggaacgcag gtctgagggc gggaatggtt tggcgctgtt tgccagatgt
      901 tgtcttccag gatttcgacg cggctgagtt tttccagtac gatgcatcgc cagttggagg
      961 gtgagccggg cggttcgagg cccctctggc tttcgccgcc gtattggtag cataggactc
     1021 gcaactgccc cgcgttgttt cgcccaaggc gatgtgggca cagcaggcgc cgccggccgt
     1081 gatacactgc cgcgatcggc cgccgtccgg ttacggccct ccaaacgatc gcatagattt
     1141 cttctaccgt ttgaatcttc gcccggacaa cgcccatttc ttacccaccg cggacctcca
     1201 cctttccctc gttaatgcgg gcttccagac acggctgcgg atgcgttgcc gggccttctc
     1261 gaacgctgcc gtcctgcatc gaaaacagcg agccatgcca ggggcaacgc acgcagtcgc
     1321 cgtcctccac ctgtccttcc gccagcggac cgcccaggtg cgaacaggtc tccgccaggg
     1381 caaagatctg atcgccgcgc ttcagcagca caaccggaac accgcccgca tcggcgcgtt
     1441 tgagctcgcc ctcgttcagt tcggaagcgt ccagcaccgg agtaaaatcc tgtggaaact
     1501 ctttgggcgc atgatccacg ccgatctttt cacggtatac caggtggccg cccaggtgcg
     1561 ctgccatgcc tgcggcggca tagcccagga tggccagccg ccggcccgat tcgcgatcgt
     1621 gccgctggcg gcaaatatag gaggcagcga agaggcccgc gccaatggta ttcaacaggc
     1681 cgtgtacaag cccgatgcga tgcggcttac cggtgatttt gtgccagtcc gtgaagccgg
     1741 tgatggcgga ccctactgca cccatcaagc cgagtgcgac cgcggcatcg gcgccgcgct
     1801 tcagcccatc accgccatcc cgcgagtcca gcgcgtcaag cacaacggcg gctgtccagg
     1861 caccgagtgg aatgtccgtc aaaaccggat gcagcggatg ccccagccat gtgccataca
     1921 gcgcgttggc gactgcgggc cccgcgctct caaccgctgt ctcgaccgcc tgttgaaccg
     1981 tctcttccac gggttcgagc cagtcctgcc gctcgacgaa tcggacgatt tcagatgaca
     2041 taaagcccca cagataggac tcaacccctg attcgccgtt tcaattaact ttggcgagac
     2101 ggcgcaaaag ctcgagggtc tcgttcagcc agcgctcggg ccgcgaggaa aatccggcgt
     2161 gctcgtatcg aatggttccg tcgcggtcga cgatccacgt gcgtggatac atcgtgaccc
     2221 gcatacggtc ctgaatgaat tcgcgagccg gaatcacggg aaacgtgtag ccgtagcgct
     2281 tcaggaacgc gggcaggctt tcagcatcct catcgacgtt gaaggtgacg aactgcattt
     2341 ccccgccggg ttccatttgc cggtacagtt tttcgagatg gggcagttcg acgagacagg
     2401 gcccgcacca cgttgcccac aggttcacga gcgttacttt gccccgaaag tcagtggcac
     2461 tccacgatct tccagccagg tcgcgcaact cgaaaccgat taacggctgc gatgcggatt
     2521 gccagccgcc cgcgggttcg cgaattccgg cccaggcgcg aaacccctct tccgtaccgc
     2581 ccatttcccg ccacagctcc aacattttgt cgatacgggc gaaccgttct gcgaaatatt
     2641 cccaccccgt gcggttctcg ctttccaggc gatagtaggc ggctgcgtcg agctttcgtc
     2701 cttccgcttc ggccagcctt gccatggaga gccagtagtc ggcctgccgg ccgccagact
     2761 ccgctgcccg ctcttcggtc ccgcccgcca gccactgctt catttcggcc agcacggccc
     2821 gtgcctcttc aaactcacga agctcgacga gtgcgccggt gagatccggc cggccggccc
     2881 agtatctttg cgcgtcggcg gcgtcatgta ccagatcggt tttcatgtcg cgaagcacct
     2941 gccgcgcttt cccgggctcg ccggagtgca tatagaagcg gacgagcacc aggcgtccgt
     3001 gagaccgtgt ctcatccaac tcccggcgtg ccgcagcccg gaaatcatct gacatccagc
     3061 cgggacggat atccgtcaag gcgcgaatct cggcctcgcg aatcgcctcc ccgacaatct
     3121 ccggaatttt ctctgttgca atgttgcgcg cgagaaaagt cgcggcaata tcaactcgtg
     3181 gcgacagagt acggctcgat ccattccggg caacagccgt caacgtaccg gaacccaccg
     3241 cttccacctc ctggtccggc aggtccggca gctcgcgggc agccttccag cgcaaagccc
     3301 aagcttctgg cgacatcggc cacgtgcgaa tccattccgc gcttgcacgg tagagttgcc
     3361 ggtaataggc tgctacggtt tcgcgcgagt cgttgatggc cggctgaagg tgagagtcgc
     3421 gccattcatc gagcgtgaga cgtgcggccg tgaacgaatg cggcagccat ttcgcgatct
     3481 cgcctcgcgc ccattcccaa cccgcccgat ctcctgttat ctcgtacgtc tccagcagcg
     3541 ccggaagcaa cgcgttgcgg cggcgcgtat ccagcgagcg aagacgcgct gcgtcttccc
     3601 ggatacgttt caacagcgcc gcacgttcgg ccgggccggc atctgcgatt tcgagctccc
     3661 acagatagtg gtaatccgcc aaacgcgacg gtaatttcga cgcactcaat tctttgcgcg
     3721 cagcttccgc cacggcgcgc cgcaattccg gagcgcctgc ttccatgagg gcggtgtagg
     3781 aagcctcatt cagcgttccg gggcaaagcc ggaaaaaatg ctccaggtgc tggcggccgc
     3841 ctttcgtgtg tgcgcctgcc agcgccagat gcggccatgg gaactccgca tgttgcccgg
     3901 ctaactgttc gaacagttcg accgcctggc gcgtcctgcg gcctaccagc gagcgtgcgt
     3961 acaggtaacg cgcggccacg ctgccggcgt gctccgaggc aagcttgcgg tactcttcga
     4021 tgaccaggtc tcgcaccggc tcggagagtt cgttggcaat gtggtcctga tactcgtggt
     4081 gtagcaccac gtcatcggga taagccattg ccgcggcacg caggagggca aggcgttggt
     4141 cgcggtcgag gcgcgcatcg tcgaaagcaa ggcgtgcctc gcgcaacagc gggtccactt
     4201 caggccggaa atcgcaagcg ccccaagcgg ccgtcaccgt caggatgata gacagcgcag
     4261 gccacaaacc cgtcaggctt ctttcgccgt catgctttcg ggatcccaac ggtacactcg
     4321 ctgcctgctc gcgctcatat tgcacaggag ggccggcgcg gctgtacgca taccgaacac
     4381 agcgtcctcg agaaacagct tgcggctgcg aaccgcggcg tagaagtttt tgtggtgctc
     4441 caggtgggca ttgtgcgaac gcggcggctc aaaggtttct tcccggtccg gacgcatcgc
     4501 gtcggcggaa ggacgctgtt cgggatactt ggcgcggtac tctttcagaa attgctcctg
     4561 gacagccttc gggaaggtac cgatggtgta gccgggctcg gtctcgcggg ggcgccgcga
     4621 aagagtcagc tcactcattg tggttgtcat gacaccttcg ctgccgacaa agcggaaccc
     4681 gaatttttcc gacgaaccac cgctggcgaa attcacccgt agcgcgaacg tgaattccgg
     4741 gtgcgcggac gtcttggggt aatccatcgt ggccagcatg acgtcggggg cgtcgcggcc
     4801 gtctttccag aatcgtaacc cgcccgtcgc gtagacacgg ttcggtccca tcgatcccgt
     4861 agcaaaatgc agtccggaaa gcaggtggac gaagagatcg cctgccacac ccgtcccgta
     4921 gtcgttatag ttgcgccagc ggaacagccg tatcggctcg aacggccgtt tcggtgcatt
     4981 ccccagaaaa cggtcccaat cgatccgctc cggcgtcgcg tccagtggaa tcgaatactg
     5041 ccaggcaccc acggcggtgt tgcggtcgag ccaagcttcc accatgttca cttcgcccaa
     5101 agcgcccgcg gccagaagct gccgggcctt gtcgaaaatc atcgagctga cgtactggct
     5161 gccaacctga aacatccggc cgctcttctt ctgtgcctcg atgacgcgct taccttcgtc
     5221 gaccgaatgg atcatcggct tttcgcagta cacgtccttg ccggcattca gagccaggat
     5281 ggagatgggc gcgtgccagt gatcgggagt accgatgatc accgcatcca catctttccg
     5341 gttgagaact tcgcggaaat cgcgcgtggt gtagagatcg ttgccccaga tctccttcat
     5401 gcgctcgagc cgcccgtcat agatatcgca ggccgccacg agtttcacac ccggaagcga
     5461 agtggcatat cgggcgtcgc cttgccccat gccgcccgca ccgatgagcg cgatctgaat
     5521 agtatcgttt gcctgaaagc tcatgtcctc aacctttcac cgtcatcgtc tcgggatccc
     5581 accggcattg cttctgctcg aagtagctta ccagagacag cagcgctggc ccggctgcgc
     5641 ggaaaccgaa caccgcgtct tccacaacgg gcttgcgaga gcggacggct tcgaagaaat
     5701 tccggaaatg atcgacgctg tcgctgtagc cgcgcggggg aagataagtt tcctccgcgg
     5761 ccggccgcat cgaatcggcc gtcgccgctg tctcgggata ctt
//
LOCUS       DASTMK010000530         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_218789, whole genome shotgun sequence.
ACCESSION   DASTMK010000530 DASTMK010000000
VERSION     DASTMK010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218789"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFQ79_21925"
     CDS             <1..639
                     /locus_tag="VFQ79_21925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0142396.1"
                     /translation="NSLLNRPVRTALPPPEDLSAAALDATLDRLYEFARANAPAIQRD
                     QKMVERSQLAVNLARKGYYPDYTLSGGYYNMGRMPDMYEFRVDVDLPLYFWRKQRKEV
                     AEQSAMASEARHTLQSTANDLEYRIRDDYQMAQTSRRLMDVYRKTVLPQANLTLESSL
                     PSYETGAVDFLTLLMNLDSILETEMNYQEEFLNYHLALIRLQEMTGMKLLED"
     gene            644..2038
                     /locus_tag="VFQ79_21930"
     CDS             644..2038
                     /locus_tag="VFQ79_21930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0142397.1"
                     /translation="MKKVFWAVAILLTIAVAFLVGRWQGAGPAAPDEKGGRKILYYVD
                     PMHPAYKSDKPGIAPDCGMRLEPVYADGGGAGTPADNRKVLYYQDPQDPAYKSDKPGL
                     NPETGNDLEPVYADAPPPAALQISPEKQRLIGVQFGVVEWGTTASSLRTVGKVAADER
                     RIQKVNSKVEGWIDKVYADFTGRVVRKGEPLLTLYSPEMLATQQEYLLAVKARKVLAD
                     SEVSGIAAQSESLLGAARKRLELWDLNDAQIDEIARTGKPVKNITLYSPAGGFITARN
                     AYPNQRIMPDTELYEITDLSHVWIIADVFESDIPKIRMGQGATVRLSYRPGRNFAARV
                     TYILPEVDPNTRTLKVRLEAANAGMQLKPEMFVDVEFRLAAARALLVPSDAVLDSGLR
                     KTVFVDHGNGHLEPRQVETGDSFDGKTTILSGLAAGERIAISGVFLIDSEAQLKGVSA
                     TAPAPAPPGAHKHD"
     gene            2031..>3745
                     /locus_tag="VFQ79_21935"
     CDS             2031..>3745
                     /locus_tag="VFQ79_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0142398.1"
                     /translation="MINRIIEFSAQNRLIVFILVAAGILGGWWSMMNVPLDALPDLSD
                     TQVIVYSRWDRSPDIIEDQVTYPIVTSLLGAPGVRTVRGFSDFGYSYVYIIFEEGTDI
                     YWARSRTLEYLSGVLQRLPQGVKTELGPDATGLGWVYQYALVDTSGRHSLAELRSLQD
                     WYLRYHLQAVPGVSEVAPLGGFVRQYQVVVDPNRLRAYNLSITQVVNAVRTGNDDVGG
                     RLVEFSGAEYMVRGRGYAQSAGDIEKIVLRAEGGVPVRIGDVGKVVLGPDMRRGIAEL
                     DGAGEVVSGIVVMRQDENALKVIERVKERLREIEPGLPAGVKVEGIYDRSELILASID
                     NLRSTLLEELIIVSLIIFLFLWHIPSAVIPTITIPAAILISFIPMYAMGITANIMSLG
                     GIAIAVGAMVDAAIVVVEQTHKKLEEWERGGRQGSYHRVVVDAVKEVGGPSFFALLVI
                     AVSFLPILALEAQEGRLFKPLAYTKNFAMIVAAVLAITLDPAMRLLFTHARNFDFRPR
                     WLARTANAVLVGKIHSEEQHPISRILMRAYAPVAEWSLRWTWVVIAGAILLVVATIPV
                     YQRLGS"
BASE COUNT          776 a         1086 c         1168 g          715 t
ORIGIN      
        1 aacagccttt tgaacaggcc ggtacgaacc gcgctgccac cgcccgaaga cctcagcgcg
       61 gcggcgctgg atgcgacgct ggaccggctc tatgaattcg cccgcgccaa cgcacccgcc
      121 atccagcgcg atcaaaagat ggtggagcgc agccaactcg ccgttaacct tgcacgaaag
      181 ggctactacc ccgattacac gctatcgggc ggctactaca acatgggccg catgcccgac
      241 atgtacgaat tccgggtaga tgtggatctg ccgttatact tctggcgcaa gcagcgcaag
      301 gaagttgccg aacagtccgc aatggcaagc gaagcgcggc acacgcttca aagcacggcc
      361 aacgatctcg aataccgcat ccgggacgac tatcagatgg cgcagacctc gcggcgcctt
      421 atggatgtct accggaagac cgtcctgccg caagcgaacc tgacgcttga atcctcgctg
      481 ccgtcgtatg agacgggtgc tgtcgatttt ctgacgctcc tgatgaatct cgattcgatc
      541 ctggagacgg agatgaacta ccaggaagag ttcctgaatt atcatctcgc gctgatccgg
      601 ttacaggaaa tgaccggcat gaagttgctg gaggactgag ttcatgaaga aagtcttttg
      661 ggccgtagct attcttctca ccatcgccgt agccttcctg gtggggcggt ggcagggcgc
      721 cgggccggcg gcgcctgacg aaaagggcgg gcgcaagatc ctctactatg tcgatcccat
      781 gcaccccgcc tataagtccg acaagcccgg catcgcaccc gattgcggca tgcggctgga
      841 gcctgtgtat gccgacggcg gaggagcggg aactccggcg gacaaccgca aggtgctcta
      901 ctaccaggac ccgcaggacc cagcctacaa atccgacaag cccggactga atccggaaac
      961 cggcaacgat ctggaaccgg tctacgcgga tgctcctcca ccggcggcgc tacaaatttc
     1021 gccggagaaa cagcggctga tcggagtgca atttggagtg gtggaatggg gcaccaccgc
     1081 aagcagcctg cgaaccgtcg gcaaagtggc cgccgacgag cggcgcattc agaaagtgaa
     1141 ctcgaaagtg gagggctgga tcgacaaagt ctatgccgat ttcaccgggc gggtggttcg
     1201 gaagggcgag cccctgctga ccctttacag cccggagatg ctcgcgacgc aacaggaata
     1261 tctgctcgcc gtgaaggcga gaaaggttct cgcggacagc gaagtatcgg ggattgccgc
     1321 ccaaagcgaa tcgctgctcg gagcggcgcg taagcgcctc gaactttggg atttgaacga
     1381 cgctcagatc gacgagatcg cacgcacggg aaaaccggtc aagaacatca ccctgtactc
     1441 gcctgccggc ggatttataa cggcgcgcaa tgcctatccg aatcagcgga tcatgcccga
     1501 cacggaactc tacgagatca ccgacctgag tcacgtctgg atcattgccg atgtgtttga
     1561 gtccgacatc ccgaagatcc gcatggggca gggcgcgacg gtgcgcctgt cctaccggcc
     1621 aggcagaaac tttgcggcgc gggttaccta cattcttccc gaagtggatc cgaacaccag
     1681 gacgctgaag gtgcgcctgg aagcagcaaa cgcgggcatg cagttgaagc ccgaaatgtt
     1741 tgtcgatgtc gagttccggt tggccgcggc ccgcgcgctg ctggttccct cagacgcggt
     1801 gctcgattcc ggcctccgaa aaaccgtttt tgtggaccac ggcaacggac atctggagcc
     1861 gaggcaggta gagaccggag attctttcga tggcaaaacg acgattctga gcggactcgc
     1921 ggccggagag cggatcgcaa tctcgggtgt cttcctcatc gattctgagg cgcaactcaa
     1981 aggagtgagc gccacggcgc cggcaccggc accgccagga gcccataaac atgattaacc
     2041 ggatcatcga gttctcagcg cagaaccgcc tgatcgtgtt catcctggta gccgcgggca
     2101 ttctgggcgg gtggtggtcc atgatgaatg tcccgctcga tgctctgccg gacctcagcg
     2161 acacgcaggt gatcgtctat tcgcggtggg accggagccc ggacattatc gaagaccagg
     2221 taacctatcc gatcgtcacc agcctgttgg gagcgcccgg agtgcggacc gttcgcggat
     2281 tttccgactt cggctactca tacgtctaca tcattttcga agaaggaacc gatatttact
     2341 gggctcgctc gcggactctc gaatatcttt ccggcgttct gcaaaggctg ccgcaaggag
     2401 tgaaaacgga actggggccg gacgcgacgg ggctcggctg ggtgtatcaa tacgcgctgg
     2461 tggacacctc gggccggcat agcctggcgg aattgcggtc gctgcaggac tggtatctgc
     2521 gttaccacct gcaagcggtg cccggcgttt ccgaagtcgc gccgctgggg ggattcgtac
     2581 gccaatacca ggtcgttgta gacccgaacc ggctgcgcgc ttacaacctt tccatcacgc
     2641 aagtggtgaa tgcggttcgt accggaaacg acgacgtggg gggccgcctc gtggagttta
     2701 gcggcgccga gtacatggtg cgcggacgcg gttacgccca atccgccggc gacatcgaga
     2761 agatcgtatt gcgcgccgaa ggcggcgtgc cggtgcgcat tggcgatgtc ggcaaagtag
     2821 ttctcggccc ggacatgcgg cgcgggatcg cggagctcga cggcgctggc gaagtggttt
     2881 ccggcattgt cgtgatgcgc caggacgaga acgctctgaa ggtgatcgag cgcgttaaag
     2941 agaggcttcg ggagatagaa ccgggcctgc cggcgggtgt gaaagtagag ggcatctacg
     3001 accgctccga gctgattctc gcgtcgatag acaatctacg atcgactctg ctcgaagagc
     3061 tgatcatcgt ctcgctcatc atctttcttt ttctatggca cattccgagc gccgttattc
     3121 caaccatcac cattcccgca gcgatcctga tctcctttat tccaatgtat gcgatgggca
     3181 tcaccgccaa tatcatgtcg ctcggcggaa tcgccatagc ggtcggggcc atggtggatg
     3241 ccgccatcgt ggtggtcgaa cagacgcaca agaaactgga ggaatgggag cgcggcgggc
     3301 ggcaggggag ctatcaccgc gttgtcgttg acgcggtaaa ggaagtcggc ggaccaagct
     3361 tcttcgcgtt gctggtgatt gctgtttcgt ttctgcccat cctggcgctg gaggcgcagg
     3421 agggacggct tttcaaacct ctcgcctata cgaaaaactt cgccatgatc gtggcggcgg
     3481 tgctggcgat aacactggac cctgccatgc ggctgctgtt cacgcacgcg cggaacttcg
     3541 acttccggcc gcgttggctt gcacgcacgg cgaacgcggt gctggtagga aagatccatt
     3601 ccgaagaaca gcaccccatc agccggatcc tcatgcgggc gtatgcgccc gttgcggagt
     3661 ggtcactgcg atggacatgg gtggtgattg ccggggcgat ccttctcgtc gttgcgacca
     3721 ttccggtgta tcagaggctt ggctc
//
LOCUS       DASTMK010000531        20496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_443372, whole genome shotgun sequence.
ACCESSION   DASTMK010000531 DASTMK010000000
VERSION     DASTMK010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20496
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443372"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFQ79_21940"
     CDS             complement(<1..188)
                     /locus_tag="VFQ79_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142399.1"
                     /translation="MNFLTGITNLTNWTGNVIMPTLAALFFALGVLRYAHGQAHTYAP
                     WAGFLCLMVSGVLRGIEK"
     gene            complement(203..865)
                     /locus_tag="VFQ79_21945"
     CDS             complement(203..865)
                     /locus_tag="VFQ79_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142400.1"
                     /translation="MAVPQFVLDAIRFLLALAAPAALVALVLAGIALRREGTTSFSVG
                     GGGFSKWMFWAIVMITLPQVFGWFAFWGVGAPLPSGGIGNGWMSAFTNDAVIFVNTFV
                     IGRLVPVLAAWMVLRSVIDWSEGYTPLASILGAMFLLAIPASAALLQGWNDGTRFGTA
                     AVLEGAWTYTASRIMPIAAGLAVIGAVVNFAFGRPAIRLIGSAAAFLTVSALWRLVVS
                     MM"
     gene            complement(956..1303)
                     /locus_tag="VFQ79_21950"
     CDS             complement(956..1303)
                     /locus_tag="VFQ79_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142401.1"
                     /translation="MNQFAKRVVVALALCLVIVQSVAIAQNLGAAPQVNVAVQGQTGA
                     QPEGAFLNFVNWIGNVIAPVGAGVAVLGAIGSYATGRGTFRWMVTAAGLLAISGVTRL
                     IEFWIAQGGGGVS"
     gene            complement(1512..3551)
                     /locus_tag="VFQ79_21955"
     CDS             complement(1512..3551)
                     /locus_tag="VFQ79_21955"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0142402.1"
                     /translation="MRRDYQATSPLLWVATQFGRFLGDGYREIRGLIASNAPDNLKKA
                     FTGSMAALILALLTVIVIASQLHPVAVILAVVCVLLIVYGRTLFKRLALFAGYFLCGF
                     VLFIGAGLITTVPLEKSYPLLAKAIAVGLFVVLCLVIPALLSMRPRLEIAASGASWAA
                     SGDQLRKPALTFADVGGMEEAKKQIRELVQANLNGKKFGQYGVFRNGILLHGPRGTGK
                     TFLAEATAGEFGLKYLYVSASSLVGRYVGTTEGNIESAFAQAKTERPIIIFIDEIDAV
                     GTKRQQLGEADDTGGAARMYNSTTNRLMGCIDEVRKTTGVVLMAATNFYDGLDRALIR
                     EGRFDLHVRLDLPNKEERALIFEAQLAKRPSRRLDVLGFAKRTPGWSAAKIGTLVDRA
                     AFFAAEEQRKIEERDLTRALADTGGKDRGAFKEVEWEDVVLSPDTEADLRNLVRLMDP
                     VYGERLKLAMPTGLLLIGSPGTGKTMIARLIATQTKRSFYPITAADILGGATGASVKK
                     LTELFARAQENSPSIIFIDEMDGLLPRNNGFQSQHDIQLVEQARSLISELEPQHNVFL
                     IGTTNHLDSIDAAILRGGRFSEKIEIGTPHESGYQCLLKKHLDGVRLVEALSVELLAE
                     RMKGISPADLEAICHSAKRMAMRRMAEDAEELPPLTWNDFTEAMKRVQVQFSERS"
     gene            complement(3605..3955)
                     /locus_tag="VFQ79_21960"
     CDS             complement(3605..3955)
                     /locus_tag="VFQ79_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142403.1"
                     /translation="MGANWKPLEAKLGALRCAGFMFMGRLNGVNQYKHGISRRYLFLD
                     DEGRAYESAGRDEFREIAFEEALGRVEAPLGVLGATLETPYDAAFIGRKAEALKAAGI
                     ETLRILIEPEDGTV"
     gene            complement(3963..5507)
                     /locus_tag="VFQ79_21965"
     CDS             complement(3963..5507)
                     /locus_tag="VFQ79_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142404.1"
                     /translation="MAPDDRIHDEKLERETSEQDDPTLTTRVSRTGSTEEESKPVESS
                     PAEAPVASSGIVQQIWHRIKEERSRPKRVEINTRGQNNMDRSKAFLVLATAVVLCGFA
                     FLALFSTSSSEKRAQDRRTKPSLGRPENATSQQAGPAGSTIPLLSADQGAGDQNAEQL
                     SPDDILATSRRAQVGNQQPPAKAPNEYALNQVPPVNDPALEAYRRQNNYAYTPPPPPP
                     TTTAVAPVPQPMNESEGLKKSSLVFVRNTTSATVMNASQSTAQPAVIERKGVSTLMPT
                     GSRLVARLQTAVSSAVKTPAIAVIEYNYERNGEIVIPAGTKAIGQMAQVNQNGQVGLR
                     FTALEMPDGTTERIEGSGVSLDYGPLKGVVNGRNGVKRALVRSITGVGTMAAYLVGGR
                     GYGGLTGPIDQSVLLRERIASNIGLAGEQELMAMAYTQNVVVMLPGNTRFYIVLQEAV
                     TAPNRPDLTPAGAAGGRSNIASVEGQALPTAAELRELVALKSELTRMYREVAATRAAE
                     PPAPQQ"
     gene            complement(5494..6600)
                     /locus_tag="VFQ79_21970"
     CDS             complement(5494..6600)
                     /locus_tag="VFQ79_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142405.1"
                     /translation="MTAKQWMFWILPWILAVLDLGGTAQGQTATHSLVQPQVVGRPIR
                     DGQVTTVFLAPRYVTAIRMPEPVNSVVVGDPASFSAEHSDREPNLVFVKPITTKAAQT
                     NLLISSTRGLQASLLLISRGEPKDEEQRGVDFLMRYKPAGQFMIQPADSPSVMIPQTV
                     AVGSTATVSSASSLPVPIVPASSTSRDGSKEQPASQSPPAKNSTKLDRTALDELLDRQ
                     RRAPLPVLYGQKPGISVPGKELVKTGVSEVIDQGSEVVVLFSAVNVQDHAVELMPPQI
                     QLGGKKKSGKIIRKSRWSTAEQLPVTDFRMSRRRVGPGERADGVVVFQRPSFKQSTET
                     LFLQMAESGAVDRPALAPIGFGISSFREVAYGAR"
     gene            complement(6597..7100)
                     /locus_tag="VFQ79_21975"
     CDS             complement(6597..7100)
                     /locus_tag="VFQ79_21975"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0142406.1"
                     /translation="MRFVCLFAAGAVVCTAHEDVRRAAVEWADHYAALYRVPNDLVHS
                     IIEVESAWNPRAVSTEGAVGLMQLMPATAVRFGVMNRFDIEQNIRAGVAYLACLLKLF
                     DGDLRLAAAAYLTGENRILPAGLAYSRVEVHQYVSKVARVYREKRSKRLQGKVLAENK
                     LEGGHQP"
     gene            complement(7133..8077)
                     /locus_tag="VFQ79_21980"
     CDS             complement(7133..8077)
                     /locus_tag="VFQ79_21980"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0142407.1"
                     /translation="MFSSVEELGRLLEAAKYVIDPITLQVVYLAARMQKPVIVEGPPG
                     CGKTALAQAIAAAGNTVIERLQCYEGINEEKAIGKFDSALQKLFLETQADHLEKDWDS
                     IRNNLHTLDFFVQGPLLRSLLYERSCVLLIDEVDKVDEGFEALLLEILSEWQISIPKL
                     GTIKQKSVPFVILTSNEVRRLGDPLRRRSFYLRVEFPTVEREAEILRVRSTTSNARFQ
                     RLIAGLAHALRGWQMEKPVSIAEMLELAQALEILGVQDITPEMRDTLMPMLAKTGSDR
                     KRLLLRDGFASLVHDAHRYAEEIPGDALEREMLEVTTR"
     gene            complement(8692..9594)
                     /locus_tag="VFQ79_21985"
     CDS             complement(8692..9594)
                     /locus_tag="VFQ79_21985"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0142408.1"
                     /translation="MLLRDRRPPRSRVAVITAEKYSEQLEEILPRGLRLFHLDVKGKS
                     VLLKPNFVEYIAGVEVNTNPLLVGATAAAFLKLGARSVVVGEGPGHQRDTYLVLVESG
                     LDAQLRDRRIRFVDLNRDELFKVPTRATYTGLDHFWLPRAVLASDFVVSMPKIKTHHW
                     AGVTLSMKNMFGVIPGTKYGWPKNVLHWKGIHRSILDISATVPVHFVIADGIVAMEGN
                     GPLHGTHRNLGKIVLADDPVAADFTCTRLMGLDPNRVWHLNRAATFLGNGSNHRIDLI
                     AEAVPRGTIPFNVLPEFMYLQTRV"
     gene            complement(9681..10442)
                     /locus_tag="VFQ79_21990"
     CDS             complement(9681..10442)
                     /locus_tag="VFQ79_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142409.1"
                     /translation="MSVRNRNGSWPAFDGDETSGCWATALAVLALLAVGREPAAVVSG
                     IKWLLSARGREGNWFWSWKFKTVDNRVKFNPAKYGWSWVSGTASWVIPTAFSVIALRQ
                     VRRHGLYQTAELAERIKMGIGMLLDRMCPGGGWNAGNGFAFGVPYSPYIDATAVALLA
                     LVEHEKEPGVQASLAWLLNGLAGCPSPYSMAWGILALATQRDKGGEVNVTLERSTREL
                     TGLLERTGKTDVCTLAVCVLALDASEGDNVFQVRA"
     gene            10829..11122
                     /locus_tag="VFQ79_21995"
     CDS             10829..11122
                     /locus_tag="VFQ79_21995"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0142410.1"
                     /translation="MNAKHLPQNGAPGGSALGFAVVKEELPLTKDELPLTVRDAAVYL
                     GVSIQTVYLWVERKQIPHLRVMGRNIRFLKSDLESFRATFRQEAANVASQALP"
     gene            11345..12403
                     /locus_tag="VFQ79_22000"
     CDS             11345..12403
                     /locus_tag="VFQ79_22000"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0142411.1"
                     /translation="MIRESAGTSDRQDAERFLRDRLDARDEGRLPTVLNSKQLTFGQW
                     ADWFLERRSKPPFRSAGNHQQNLNALKFLRPVFGDTALSEITAEAIENYLADRLATGR
                     RVYTKAGIQLRGKIKPATAHQEFRILSHMLNVAVKQKRLSANPCVAVEFPVSVAKSTR
                     KPHYMNSSEQAKIESAAPAYLRNVVVILSETGLRYKKELLSMKKAQVDLENGLAHIAD
                     SKTANGIGDMPLTAVAREAFQRQIDETPGSEYLFPTPKIGAKKPYLTNLRKVWAGTLK
                     RAGVPYFALYELRHTFATRLSAGGVADHMVTQMLRQGDADVFKLYSQAKLGMMREALA
                     KLDRKANERRISSTEKAS"
     gene            complement(12488..12565)
                     /locus_tag="VFQ79_22005"
     tRNA            complement(12488..12565)
                     /locus_tag="VFQ79_22005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12528..12530),aa:Pro,seq:ggg)
     gene            complement(12596..13003)
                     /locus_tag="VFQ79_22010"
     CDS             complement(12596..13003)
                     /locus_tag="VFQ79_22010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU0142412.1"
                     /translation="MPDTFELEVTTPERLLIRDQVNEAQIPAANGYLGIGPEHAPLLA
                     ELGIGELSYRVGNMRKSLVVSGGFVEVMPDHVRVLADSAEKASEIDVERARAALQRAE
                     ERLGSNATAGIDVARALNAATRAKARLEAATHR"
     gene            complement(13011..14471)
                     /gene="atpD"
                     /locus_tag="VFQ79_22015"
     CDS             complement(13011..14471)
                     /gene="atpD"
                     /locus_tag="VFQ79_22015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU0142413.1"
                     /translation="MATQAVPTVIGRVIQVAGPAVDIQFPEGQIPVINTAIRITSEGF
                     EVPAPIDIVVEVAQHIGEGRVRTIALQPTDGLVRGMKCESLGEPVTVPVGKETLGRVI
                     NVIGEPVDKMGPLNASARLPIHRPAPSFEEQSTQLKMFETGIKVIDLLEPYLLGGKIG
                     LFGGAGVGKTVVIMELINNIAMKHGGVSVFAGVGERTREGNDLWLEMQESGVLDPRDY
                     TKSKVALIYGQMTEPPGARLRVGLTGLTVAEYFRDVEGQDVLLFIDNIFRFTQAGSEV
                     SALLGRMPSAVGYQPNLATEMGELQERITSTKKGSITSVQAIYVPADDYTDPAPATTF
                     AHLDATTNLSRAISEMGIYPAVDPLASTSRILDPLILGEEHYSVAQNVKQILQRYKDL
                     QDIIAILGIDELSEDDKLIVARARKIQRFLSQPFHVAEQFTGFKGKYVKLEDSIRGFK
                     EIVDGKHDDIPEQAFYMQGTIEDVAERAEQLRKAAA"
     gene            complement(14478..15344)
                     /gene="atpG"
                     /locus_tag="VFQ79_22020"
     CDS             complement(14478..15344)
                     /gene="atpG"
                     /locus_tag="VFQ79_22020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU0142414.1"
                     /translation="MPSLIDIRRRIRSVRNTEQITKAMKMVSAAKLRRAQDRVISARP
                     YAASLRKLLQDVAGAVVAGAEDTPKHPLLARPAAEQRIQLVLLTGDRGLAGAFNSNLI
                     KASQSFLAERETMQVELLLLGRKGRDFWRRRTVPITDEYPGIFNKVLYEDARQIASRL
                     AERFSNNEIDAVYLLYNEFKSVIAQNLTLLKILPVEIPERENPIDYIYEQPPEELLNA
                     LLPRYAHIAVYQAMLESSAAEHAARMTAMDAASSNAAEVIENLTLYMNRVRQASITRE
                     IIEIVSGAAALE"
     gene            complement(15357..16883)
                     /gene="atpA"
                     /locus_tag="VFQ79_22025"
     CDS             complement(15357..16883)
                     /gene="atpA"
                     /locus_tag="VFQ79_22025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU0142415.1"
                     /translation="MAQVRADEIARVLREEIENYDRVVNISETGSVISVGDGIARIHG
                     LERVMAGELIEFPHHVSGLALNLEEDQVGAVLLGDFTEIREGDEVRRTGRIMSVPVGD
                     GLVGRVVNALGEPIDGKGPVETSEFNAIERLAPGVVDRSPVKEPLQTGIKAIDGMIPI
                     GRGQRELIIGDRQTGKTAIALDTIINQKGGDVICIYVAIGQKRSTVAQVVKTLEDHGA
                     MDYTIVVAATASDPAPMQYLAPYSGCSMGEYFRDSGRHALCIYDDLSKHAAAYREISL
                     LLRRPPGREAFPGDVFYLHSRLLERAAKLNAENGGGSLTALPFIETQAGDISAYIPTN
                     VISITDGQIFLEADMFNSNIRPAVNVGLSVSRVGGNAQIKAMRQVAGSLRLDLAQYRN
                     LAAFAQFGSDLDKASQAQLNRGRRLVEILKQPQYKPLPVEKQVLILFAGINGYLDDLP
                     VESCRAFEDELYRFVDNAHGPLLQEIREKKTLDDQLRDKINAVLKELKERFTAEHKAA
                     "
     gene            complement(16923..17471)
                     /gene="atpH"
                     /locus_tag="VFQ79_22030"
     CDS             complement(16923..17471)
                     /gene="atpH"
                     /locus_tag="VFQ79_22030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU0142416.1"
                     /translation="MAHAVAGRYAKALVDLVARPSSSVSPTEAVAQLTSFARVLAESP
                     ELENVLLSPAVSSSRKRAVIGRLASDLSISKLISNFLFVLVDHRRVAELNAIRDAFDA
                     LMDERMGVTRVRVASARELNDRQRQGLRDRLSALTGNTARLEFAVDPELLGGAMARIG
                     STIYDGSVRGQLEALRRRLSAE"
     gene            complement(17474..18094)
                     /locus_tag="VFQ79_22035"
     CDS             complement(17474..18094)
                     /locus_tag="VFQ79_22035"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142417.1"
                     /translation="MKIVCSMVVIGLLVFVSAGLAQPEPSHETESEKAVEHAAAEEHD
                     TGNITLWKIVNFALLAAGLGYLTKKFGLPFFQARTLSIQSGIAEASRLRQDAEARARE
                     LEQRMASLATEIDHMRENARQEISAEAGRVRMETEQQLSKVQAHAEQEIEAAAKASRQ
                     ELKAYSAELALRLAESKIRARLTPDTQDALVDSFIADLERPRMEAQ"
     gene            complement(18091..18477)
                     /locus_tag="VFQ79_22040"
     CDS             complement(18091..18477)
                     /locus_tag="VFQ79_22040"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142418.1"
                     /translation="MIFLYFYLKRVFFGPLEKLLETRRAATEGARAAAEASFARAGEM
                     AAQYEQAIRQARAEIYSEQEKMRRDWREEQNARVREARAEAERQVERAREQLARDVAD
                     SRERLAAESEALADRIIERILAGSVQ"
     gene            18700..20319
                     /locus_tag="VFQ79_22045"
     CDS             18700..20319
                     /locus_tag="VFQ79_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEU0142419.1"
                     /translation="MWRQNYIPLADSLGWSALVAALPVFALLYLIGVRRKPAWIAALA
                     GLGAAAVVALFLYRMPFGTMLSAIGYGAAFGLFPIGWIIFWAILLYRITLETGNFEIL
                     KDSVGSLTHDRRLQALLIAFAFGAFVEGAAGFGTPVAVAAAMLTGLGFSPFYAAGICL
                     LANTAPVAFGSIGIPVITLAGITGLPLLELSAWVGRICAPVSLFVPAYLILVMGGWKA
                     LRGVAPAALLCGASFALTQFLVSNFIGPQLTDILSSLAAIGTLVLLFRFWQPRDSFHL
                     EGEDGQTKSAAPPRHGTGKTLLAWTPYGFLVIFVLLWGMDSVKTLLNHVTFAITWPGL
                     HNVVSRIPPVVASEQAYPAVFTFNYLSAAGTACMFAALATALTLRTGMGQFIRIFTHT
                     ARQLALSMVTIAAVLGLAFLMNYSGATATLGLAFAATGALFPFFSAMLGWLGVFLTGS
                     DTSANALFGNLQVVTAGRLNLDPVLMASANSSGGVMGKMISLQSIAVAAAATGMASAD
                     EAKLFRFTLRHSIILATAIGLLTMVYAYAFG"
BASE COUNT         3982 a         6396 c         5807 g         4311 t
ORIGIN      
        1 aatttctcga ttccccgcaa gacacccgaa accatcaggc agagaaaacc cgcccacggt
       61 gcataggtgt gcgcctggcc gtgcgcgtag cgaaggacgc cgagcgcgaa gaacaacgcg
      121 gcgagcgtcg gcatgatgac gttgcccgtc caattcgtca ggttcgtaat gccagtgagg
      181 aagttcatgc ggcctcaagt tgctacatca tcgaaacgac caagcgccat agagcggaga
      241 cggtcaggaa cgccgcggcg gagccgatga gccggatggc tggccgccca aatgcgaagt
      301 tcacaacggc gccgatgaca gccagtccgg ctgcgattgg catgatccgg ctggcggtgt
      361 acgtccaagc tccttcgagg accgcagccg tcccaaagcg cgtcccgtcg ttccaacctt
      421 ggaggagagc tgcgcttgcg gggattgcca ataaaaacat ggcgccgagg atcgaggcta
      481 acggagtgta gccctcgctc cagtcgatca ccgaccgaag aaccatccat gccgcgagga
      541 ccggcaccaa acggccgatc acgaaggtgt tcacgaagat cactgcgtca ttcgtaaatg
      601 ctgacatcca tccgttgccg atgcctccac tgggaagtgg cgcaccgaca ccccaaaagg
      661 cgaaccatcc gaatacctgc gggagggtga tcatcacgat ggcccagaac atccatttcg
      721 agaatccacc tccgcccacc gaaaacgaag tcgttccctc gcggcgcaac gcaattcccg
      781 ccagaacgag ggcgacaaga gcggctggcg cagctaacgc cagcagaaat cggattgcgt
      841 cgagcacgaa ctgtggaact gccatttcgc tttcccctgt ttgggaagtc ggcaggctgg
      901 cccccgcttg gaaaccggcc tgcctacttg gttgctaaag ggtggcgacc aggcctcaag
      961 aaacgccgcc gccgccctgg gcgatccaga actcgatcaa ccgggtaaca cccgagatgg
     1021 cgagtagtcc ggctgcggtc accatccatc ggaatgtgcc ccttcccgtg gcataactac
     1081 cgatggcgcc caacaccgcg acgcccgcgc cgaccggagc gatcacgttg ccgatccagt
     1141 tgacgaagtt gaggaacgcg ccttccggct gcgctccggt ttggccctgc acggcgacgt
     1201 tcacctgcgg ggcagctccg agattctggg cgatcgccac gctttgcacg atgacgaggc
     1261 agagtgcaag cgcgacgaca acccgttttg cgaattgatt catggtgacg gttaacctcc
     1321 atgtgattgg cattcagcgc tggtctccgc actgaagccc cggcggaaag cccgggcccg
     1381 aaagcggcta ccagatcgtg gaagcagaat agagcaaacc gcccgctgtt ttcaaaagtc
     1441 tccgaatcgg gctcttgact ggttcaaaag gggagaaaca aactcggtgg gaagaggaga
     1501 taagtcgatc gttaggatct ctcagagaac tgaacctgaa cccgcttcat cgcctcggtg
     1561 aagtcgttcc aggtgagcgg aggcaactct tcggcatcct ccgccattcg cctcatcgcc
     1621 atgcgcttcg cgctgtggca gatggcttcg agatcggccg gtgaaatacc cttcatcctt
     1681 tcggccagca gttctaccga cagcgcctca accaaccgga cgccgtcgag gtgctttttc
     1741 agcaggcact ggtaaccgct ctcgtggggt gtgccgatct cgatcttttc ggaaaaacgg
     1801 cctccgcgaa ggatggctgc gtcaatgctg tcgaggtggt ttgtcgtccc gatcaggaaa
     1861 acattgtgct gcggctcaag ctcactgatg aggctccgcg cctgctcgac cagttggatg
     1921 tcgtgctgtg actgaaaacc gttgttgcgg ggcaacaggc catccatctc gtcgatgaag
     1981 atgatcgacg ggctgttctc ctgcgctcgg gcaaagagtt cggtgagctt ctttacagat
     2041 gcgccggttg cgccgccgag gatgtcagcg gccgtgatcg ggtagaagct ccgtttcgtc
     2101 tgagtcgcga tcagccgcgc aatcattgtc ttgccggtcc ctggggaccc gatgagcagc
     2161 agaccagtgg gcatggccaa cttgagacgc tctccatata cggggtccat caggcgaacc
     2221 agattccgga gatccgcctc ggtatcaggg gacagaacaa cgtcctccca ttcgacttcc
     2281 ttgaaggcgc cgcggtcctt gcccccggtg tctgccaacg ctcgtgtcag gtctcgttct
     2341 tcgatctttc tttgctcttc ggctgcgaag aacgccgcgc ggtccaccaa ggtgccaatt
     2401 ttggcggcgc tccaacccgg cgttcttttc gcaaacccca ggacatcaag ccgcctcgat
     2461 ggccgcttgg ccaactgggc ctcaaagatc agagccctct cttccttgtt gggcagatcg
     2521 agccgaacat gcagatcgaa acggccctcc cggatcagag ctcgatctag gccatcgtag
     2581 aagttggtcg cggccatcag gacgaccccg gttgtcttgc gaacttcgtc gatacaaccc
     2641 atcaaccgat ttgtcgtcga gttgtacatt cgcgctgccc cgcctgtgtc atcagcttcg
     2701 ccgagttgtt gccttttcgt cccgacagca tcgatctcgt cgatgaatat tattatggga
     2761 cgctctgtct tcgcttgcgc aaaagctgac tcgatattgc cttctgtagt cccaacgtac
     2821 cggcctacta agctcgacgc agaaacgtac aaatacttca atccaaactc gccagcggtt
     2881 gcttccgcca ggaacgtctt ccccgttcct cgtgggccat ggagcagaat gccgttcctg
     2941 aacaccccgt actggccgaa cttcttcccg ttcaggttcg cctggaccaa ctcgcggatc
     3001 tgcttcttcg cctcttccat gcctccgacg tcggcaaagg tgagagcggg tttcctcagc
     3061 tggtcgccac ttgcggccca gctggcacca ctcgcagcga tctccagtct cggcctcatg
     3121 gacagcaggg ctgggatcac aaggcacaac acaacaaata gaccaacagc gattgccttg
     3181 gccagaagcg ggtagctctt ctcaagcgga actgtcgtga tcagcccggc gccaatgaac
     3241 agcacaaagc cacacagaaa gtagcccgca aacagcgcca gccgtttgaa cagagtcctg
     3301 ccatacacga tgagcagaac gcagacaacg gcgagaatca ccgcgacagg atggagttgg
     3361 gaggcgatca cgatcacagt cagcagggcg agaatcagcg cagccatgct gcccgtgaag
     3421 gctttcttga gattgtccgg cgcgtttgaa gcgatgagcc cgcggatctc ccggtatccg
     3481 tcgcccagaa atcgcccaaa ttgcgtcgct acccagagga gcggactggt cgcttggtaa
     3541 tcacgccgca caaccgactc cttgggctac tttacctcat ctggaatgct ctcaccacag
     3601 gccttcagac ggttccgtct tccggttcga tgaggatacg taacgtctcg attccggccg
     3661 ctttcaatgc ctctgctttc cgcccgatga aagcggcatc atatggggtt tcgagcgttg
     3721 cgccgagcac tcccagtggt gcttccaccc gccccagagc ctcctcgaag gcgatctctc
     3781 gaaactcatc cctcccagcg gattcgtagg cccgcccctc gtcgtcgagg aacaagtagc
     3841 gccggctgat tccgtgtttg tactgattaa ccccattcaa tcggcccatg aacatgaacc
     3901 cggcgcaccg gagcgcgccg agcttcgctt cgagcggttt ccagttcgcg cccatggtct
     3961 cctcactgct gcggagccgg tggctccgct gcccgcgtgg cagccacttc ccggtacatt
     4021 cgggtgagtt cgctcttcag tgccaccagt tcccgtagct cagccgccgt gggcaaagct
     4081 tgtccttcta cactcgcgat attgctccgg ccacccgccg ccccggccgg cgtcaggtcg
     4141 ggcctatttg gagccgtaac cgcttcctga aggacgatgt agaaccgtgt gttgcccgga
     4201 agcatgacga cgacgttttg ggtgtatgcc attgccatca attcctgctc acccgcgagg
     4261 ccgatgtttg atgcgattcg ctcccgcagc agaacactct ggtcgatcgg gcccgttagc
     4321 ccaccgtaac cgcggccacc caccaggtag gccgccatcg tcccaacgcc ggtgatcgac
     4381 cgaaccaggg cgcgcttcac cccattccgt ccattcacga cacccttcag tggaccgtag
     4441 tccagactga caccgctgcc ttcgatcctc tcggtggtgc catcgggcat ttcgagtgcc
     4501 gtgaagcgaa ggcccacttg gccattctgg ttgacttgcg ccatctgtcc gatggccttc
     4561 gtgcctgccg gaatgacgat ctccccattc cgctcgtagt tatactcgat caccgcaatg
     4621 gccggcgtct tcacggcgct actgacagcg gtctgtagtc gtgccaccaa tcggctgccg
     4681 gtcggcatca acgtcgagac cccctttcgc tcaataacgg caggctgagc cgtcgactgg
     4741 ctggcgttca tcaccgttgc cgaagtcgtg tttcgcacaa atacaaggga ggatttcttc
     4801 aggccctccg actcgttcat tggttgtggg actggtgcga cggcagtggt tgttggtgga
     4861 ggcggcggtg gagtgtacgc gtagttgttc tggcgccggt acgcttcaag ggctggatca
     4921 ttcacgggag gcacttggtt cagcgcatac tcgttgggcg ccttcgccgg cggctgctgg
     4981 ttcccgacct gtgcgcggcg cgacgtcgcc aggatgtcgt ccggactcag ttgctcggca
     5041 ttttgatcgc cggcgccttg gtcggcactg agcagcggaa ttgtggaccc ggcaggcccc
     5101 gcctgttggg aggttgcgtt ttccggtctg cccaagcttg gcttcgtccg ccggtcctgc
     5161 gccctttttt ccgagcttga ggtcgagaat agggcgagaa aggcgaagcc gcacaacacg
     5221 accgccgtgg caagaaccag aaacgcctta ctccggtcca tattgttctg gccacgggtg
     5281 ttgatctcaa ctcgtttggg tcggctgcgt tcttccttga ttcgatgcca gatctgttgc
     5341 acaatgccgc tcgatgctac cggcgcttca gccgggctgc tctccaccgg cttcgattcc
     5401 tcctcagtgc tgccagttcg gctcactcgc gtcgtcagcg tgggatcgtc ctgctcactc
     5461 gtttctcgct caagcttctc atcgtgaatg cggtcatcgg gcgccataag ccacctcacg
     5521 aaaactgcta attccaaaac caattggagc caaggccgga cgatctacag cgcccgactc
     5581 ggccatctga aggaacagcg tctcggtcga ctgcttgaaa gatggccgct ggaagaccac
     5641 cacgccatcg gctcgctcgc ctgggccgac gcggcgccgg ctcatccgga agtccgtaac
     5701 gggcaactgc tcggccgtgg accagcgcga cttgcgaatg atcttcccgc tcttcttctt
     5761 gccgccgagc tgaatctgcg gaggcatcaa ttcgaccgca tggtcctgaa cgttcacggc
     5821 tgagaacaag accacgacct cgctaccttg atcgatcacc tcactgaccc cagtcttgac
     5881 tagttccttc cccggtaccg agatacccgg cttctgcccg tagaggacgg gcaacggcgc
     5941 acggcgttgg cggtctagaa gctcatcgag agccgtccga tccaacttcg ttgaattctt
     6001 cgcaggcggt gactgcgagg ccggttgttc cttcgagccg tcgcgagagg ttgacgaggc
     6061 gggcacaatc ggaactggca gactcgaggc ggaagacaca gttgctgtgc tgccaaccgc
     6121 tacggtttgc gggatcataa cggatggaga gtcagccggt tggatcatga attggccggc
     6181 gggcttatac cgcatcagga aatccacccc gcgttgctcc tcgtccttcg gttcgccacg
     6241 gctgatcaga agcaggcttg cctgcaggcc gcgagtcgac gatatgagca agttggtttg
     6301 cgccgccttt gttgtgattg gctttacgaa caccaggttc ggttcacgat ccgaatgctc
     6361 tgccgagaac gaagccgggt cgccgaccac caccgaatta accggctccg gcatccggat
     6421 cgccgtcacg taacggggcg ccagaaacac ggtcgtcacc tggccgtctc gaatcggtcg
     6481 tccgacaacc tgcggttgta ctagcgagtg cgtcgccgtc tggccttgcg ctgttccccc
     6541 gaggtccagc accgccagaa tccaaggtag gatccagaac atccactgtt tcgctgtcat
     6601 ggctgatgac ctccttccag tttgttttcg gcgagaacct ttccttgcag gcgtttcgag
     6661 cgcttctctc ggtacactcg cgccaccttg ctcacgtact gatgaacctc aacgcgcgag
     6721 tatgcgaggc cggccggcag aattcggttc tcgcccgtca gataggctgc cgcggctaag
     6781 cgtagatcac catcgaacaa cttcagcaag caggcgaggt aagctactcc ggctcggatg
     6841 ttctgttcga tatcgaaacg gttcatgacc ccgaatctca ctgccgtcgc gggcatgagt
     6901 tgcatcaacc ccactgcccc ttcggtcgac acggcacgtg gattccaggc tgactcgacc
     6961 tcgatgatcg agtggacgag atcattcggt acgcgataga gggccgcata atgatccgcc
     7021 cactccacag cggccctgcg cacgtcctcg tgtgcggtgc acacaaccgc acccgccgcg
     7081 aataggcaaa caaatctcac acgcgctgtc atagccattg agcctgctca gactatcgag
     7141 tcgttacctc caacatttcc ctctccagtg cgtcccccgg aatctcttcg gcatatcggt
     7201 gggcatcatg caccagcgat gcgaagccgt cccgcagcag gagccgcttc cgatccgatc
     7261 ccgttttcgc cagcattggc atcagcgtgt cacgcatctc gggcgtgatg tcttgcacgc
     7321 caagaatctc cagcgcctgc gcgagctcaa gcatctcggc gatcgaaaca ggcttctcca
     7381 tctgccagcc gcgcagagca tgggccaggc cggcgatgag ccgttggaac ctggcgtttg
     7441 aggttgtgct acgaaccctt aaaatttcgg cttcccgctc caccgtcggg aactccactc
     7501 gcagatagaa gctccggcgt cgcagggggt cgcccaggcg acggacctcg ttcgaggtca
     7561 ggatgacgaa aggaaccgac ttctgtttta tcgtccccaa cttcgggatg gagatctgcc
     7621 attccgagag gatctcgagc agcagcgctt cgaatccctc gtccaccttg tcgacctcat
     7681 cgatcagcag gacacacgac cgttcataga gcagtgatct cagcaggggc ccctgaacaa
     7741 agaaatcgag ggtatgaagg ttgttgcgga tcgaatccca gtccttttca aggtggtcag
     7801 cctgcgtttc gagaaatagc ttctggaggg cgctgtcgaa cttgccaatg gccttctctt
     7861 cgttgatgcc ttcgtaacac tggagccgtt cgatgaccgt atttccagcc gctgcgatcg
     7921 cctgagccaa tgcggtcttg ccacaaccag gcgggccttc tacgataacc ggcttctgca
     7981 tccgggcagc gaggtaaact acctgcaggg tgatgggatc gatcacgtac ttggccgctt
     8041 ccaacagtcg gccgagttct tccacagatg agaacacgaa tttcctctgg ccccgattgt
     8101 aggcggaaag ctcttttgcc ctatactctc gtttagcctc cagggaccgt tccgtcttca
     8161 ccctcaaacc acgtgcgtct ccctcgtttg gttcagacag gcgttgagtc accgtggtat
     8221 aaacaatgtt gctggacctg ttttaccccc aaactcagtc gcccactgcc actgtaaaaa
     8281 atgttcttgg acctattccc attcacgcgg cgcttgggtg ggcccgcgac tgttgtagga
     8341 aatgttgctg gacctatttc gccctcgatt gctccagtgc cgctgtaaaa actgtttctg
     8401 gacctatttc accttcggtc gtgccaacac tcctgtaaag aatgttgttg gacctattcc
     8461 gcgccacccg gaccctccat ctcagcggcg tcggaactgg cgttcagttg gttgccgacc
     8521 gcaccccgtc atcccgcgcc ccgggtaacc tgagaaatgt tcctggacct atccagaact
     8581 gccgatgcca gagaggaggg agatggtatg ggccgacgcg ttctgttggg tttgcaaggc
     8641 cgcgatggtc tcaggacctc gcacccctca gcgcctgatt tcttcgagtg cttacacgcg
     8701 agtctgaagg tacatgaatt ctggcaggac attgaaggga atcgtccccc gaggcactgc
     8761 ttccgcgatc aggtcgatcc ggtgattcga gccgttgccg aggaaagtcg cggcacgatt
     8821 gagatgccac acgcgattgg ggtcgaggcc catcaagcgt gtacaggtga agtccgcggc
     8881 cacgggatcg tcggccagga cgatctttcc aagattccga tgagttccgt gcaggggccc
     8941 gtttccctcc atcgcgacga tgccgtccgc gatgacgaag tgcaccggca ccgtcgcgct
     9001 gatatcaagg atgctccggt ggattccctt ccaatgcagg acgttcttcg gccacccgta
     9061 cttcgtgcca gggatcacgc cgaacatgtt cttcatgctg agggtgacgc cggcccaatg
     9121 gtgggtcttg atcttcggca tcgatacaac gaaatcggac gcgagcacag cccgggggag
     9181 ccaaaagtga tcgagcccgg tataggtggc tctagtcggc actttgaaca actcgtcgcg
     9241 gttcagatcg acgaatctga tcctccgatc gcgcagttgt gcgtcgagcc cgctttcgac
     9301 gagcaccaga tatgtgtccc gctgatggcc aggtccttcc cctaccacga cgctcctcgc
     9361 gcccagcttg agaaaggcag cggccgtcgc gcccacgagt aacggatttg tattgacctc
     9421 cacgccagcg atgtactcga cgaagttggg tttgagcagc acggattttc ccttcacgtc
     9481 gagatggaag agccgaagtc cccgaggaag aatttcctct agctgttccg agtacttctc
     9541 ggcggtgata accgctacgc gggaacgcgg cggccggcga tcgcgaagaa gcacttcctc
     9601 cggcaggagt cgggccgtcg ttattccacc cagagcaagc ccagtggcgc taatcagagc
     9661 tcgccttcga ccgaatgtgc tcatgcccgc acctggaaaa cgttgtcacc ttctgacgcg
     9721 tcgagtgcca agacgcaaac cgccagggtg cagacatcgg ttttgccggt acgctctagg
     9781 agacctgtca gctccctcgt ggagcgctcg agcgttacgt tgacttcacc gcccttgtcc
     9841 cgctgtgtcg ccaatgcgag gatgccccac gccatgctgt aaggagatgg acatccagcg
     9901 agcccattca agagccacgc caaggaagcc tgcacgcccg gctctttctc gtgttcaaca
     9961 agtgcaagca gggcgacggc tgtggcatca atatatggag agtagggcac gccgaacgca
    10021 aagccattgc cggcgttcca tccaccaccc gggcacatcc gatcgagcag catgccgatt
    10081 cccatcttga tgcgttcggc gagttcagcc gtttgataga ggccgtgcct cctgacctgc
    10141 cggagtgcga tcacgctgaa ggctgtcggg atgacccagc ttgctgtgcc ggacacccaa
    10201 ctccacccgt acttcgccgg attgaatttc acacggttgt cgacggtttt gaacttccag
    10261 ctccagaacc agttgccctc acggcctctt gcactcaaca accacttgat tcccgaaacc
    10321 acagccgcag gctcgcgccc tactgccagg agggccaaaa ctgcaagggc tgtcgcccag
    10381 catccgctgg tttcgtcgcc gtcgaaagcc ggccaactcc cgtttcgatt ccgaacggac
    10441 aaaagcgcct cgacggcctg ttccaaaccc cgactgcgct gctgccgcaa cgccaagata
    10501 ccaaggcaga ttgattcgac tgacgtcacg ccacggagtc ccttcgaagc gttagctcca
    10561 tcgccgcccc gctgtaatcg ctgctcaagc gcccggatta cttgttccct tctcatggtt
    10621 ggctttcgtg ctccgcacca gaagtatgcc actggggacc ttcgcactcg aattctcttg
    10681 attgcaggcg atcaaaagcg cacttccatt cgtccccttg cagccactga acggaatctc
    10741 gttgttacga tttggctggt cgccagggcc aacgcgaggt gatcaccaga cttttggcag
    10801 caaggcagcg tatgccgtac aaataggcat gaatgcaaag cacctccccc aaaacggcgc
    10861 cccaggcgga agcgcactgg gctttgccgt cgttaaagaa gaacttccgc tcaccaaaga
    10921 cgaacttccg ctcactgttc gcgatgcggc cgtgtacctg ggcgtgagta ttcagaccgt
    10981 gtacctgtgg gtcgaacgca agcagattcc tcacctccgc gttatggggc gcaacatccg
    11041 attcctgaaa tcggacctcg aatcgttccg agcaactttc cgacaggagg ccgccaatgt
    11101 cgcatctcag gctctgccgt aagcggtcgc cccacgttcc gcagccgtgc agttattctt
    11161 gtagtgagcg gaagcagatt cccctgttca gcaccaccgg ccgaccgatc tggtcggccg
    11221 aatttgaact gaatggagga atccaatgag taaacgtgga cgtccgcgca aaactgaagg
    11281 cgcagtctac agaagatcga attctgagtg ttggcaggtc cggtacaggg accagaaagg
    11341 agaaatgata cgagaatcgg cgggcaccag cgatcgtcaa gacgccgagc gcttcctccg
    11401 ggatcgcctg gatgcgcgcg acgaggggcg cctcccgacc gtcctaaaca gcaagcaact
    11461 gacatttggc caatgggccg actggttcct ggagcggcgc tcgaagccac cgttccgaag
    11521 cgctgggaac caccagcaga atctgaacgc gctgaaattc ttgagacccg tcttcgggga
    11581 taccgcgtta tcggagatca cggccgaagc aatcgagaat tacctggcgg atcgccttgc
    11641 gaccggtcgc agggtttaca cgaaggccgg catccagtta cggggcaaga tcaagccggc
    11701 cactgctcac caggagttcc gcattttgtc gcacatgctc aatgtggcgg tgaagcagaa
    11761 gcggttatca gccaaccctt gtgtggccgt cgaatttccg gtctctgtgg ccaaatccac
    11821 gcgcaagcca cattacatga actcgagcga gcaggccaag atcgagtcgg cggcgccggc
    11881 ctatctcagg aacgtcgtcg tcattctgtc cgagacgggg ctgaggtaca agaaggaact
    11941 tctctcgatg aagaaggcgc aggtcgacct ggagaacggc ctggctcaca tcgcggattc
    12001 gaagacggcg aacggcatcg gggacatgcc cctgacggcc gtggcccgcg aggctttcca
    12061 gcgccagatc gacgaaacgc cgggaagcga gtatctgttt ccaaccccga aaatcggagc
    12121 gaagaagccg tatctcacca accttcgcaa ggtctgggcg gggacgctga agagggcagg
    12181 ggtgccgtac ttcgcactct acgagttgag gcacacgttc gcgacccggc tcagcgccgg
    12241 cggcgtggcc gatcacatgg tcacgcagat gctgcgccag ggcgatgcgg acgttttcaa
    12301 gctgtacagc caggccaagc tggggatgat gcgcgaggcg ctcgcgaagc tggatcgcaa
    12361 ggccaacgag cgtcgaattt cgagcacaga gaaggcgagc tgaacgattt tatgcacatt
    12421 tattggcaca ctcgctcgcc acgaacgacg cggaggatgt tgcgtggatt gattctaaaa
    12481 gggtttttgg tcgggccgcc gggatttgaa cccgggacct cttgcacccc aagcaagcgc
    12541 gctagccagg ctgcgccacg gcccgatgtc gtggattatt ccgttattgt accagtcagc
    12601 ggtgtgtcgc cgcttccagg cgcgccttgg cgcgtgtggc agcgttcagc gcacgtgcga
    12661 cgtcgattcc tgctgtggcg ttcgatccga gtctttcctc agcgcgttga agcgcggccc
    12721 gggcacgctc gacatcgatc tccgaagcct tttcagcgct gtcggccagg acgcgtacgt
    12781 ggtccggcat tacctcgacg aaaccgcccg acaccaccag agatttccgc atgttgccga
    12841 cacgatacga cagttctcca atgcccaact ccgccagcaa cggcgcgtgc tccggcccga
    12901 tgccgagata cccattcgcg gccggaatct gggcttcatt cacctggtcg cgaatcagta
    12961 aacgctccgg cgttgtgact tccagttcga acgtatccgg catggctttg ttacgccgcc
    13021 gccttcctta actgctcggc gcgctccgct acgtcttcta tggtcccttg catgtaaaaa
    13081 gcctgctccg ggatatcgtc atgcttccca tccacgatct ccttgaatcc ccggatgctg
    13141 tcttccagtt tgacgtattt gcccttgaac cccgtgaact gctccgccac gtggaacggc
    13201 tgcgacagga atcgctggat tttacgcgcc cgcgcgacaa tcagcttgtc gtcttcggac
    13261 agctcgtcga taccaaggat cgcgatgatg tcctgcagat ctttgtaccg ctggaggatc
    13321 tgcttgacgt tctgcgcaac ggaatagtgc tcctctccaa gaatcagcgg gtcgaggatt
    13381 cgcgacgtcg acgccagcgg atccaccgcg ggataaatac ccatttccga gatggcccgg
    13441 gaaaggttcg tcgtggcatc gaggtgggcg aacgttgtcg ccggcgcggg gtccgtatag
    13501 tcgtcggccg gcacgtaaat cgcctgcacc gacgtgatgg accctttctt ggtagaggtg
    13561 atccgttcct gcaattcgcc catctcggtt gcaagattcg gctgatagcc gacggccgaa
    13621 ggcatgcgtc ccagcagagc tgagacttca gagccggcct gcgtgaaccg gaaaatgtta
    13681 tcgatgaaca gcagcacgtc ctggccttcc acgtcgcgga agtattccgc taccgtgagg
    13741 cccgtcagcc cgacacgcag gcgcgctccg ggcggttccg tcatctggcc gtaaatcagc
    13801 gccaccttgc ttttcgtata gtcgcgcgga tccagcacgc ccgactcctg catctccaac
    13861 cacaggtcat tgccttcgcg agttctctcg ccgacgccgg cgaacaccga cacgccgccg
    13921 tgcttcatgg caatgttgtt gatcaactcc atgatgacga cagtctttcc gacacccgca
    13981 ccgccaaaca ggccgatctt gccgcctagc agatacggct cgagaaggtc gatgactttg
    14041 atgcccgtct cgaacatctt gagctgggtg gactgctcct caaaactggg agcaggacga
    14101 tggatcggca ggcgtgcgct tgcattcagc ggacccatct tgtccacagg ctcgccgatc
    14161 acgttgatta cgcgtccgag agtctctttt ccaacgggca cggttaccgg ctctccaagg
    14221 ctctcgcatt tcatgccccg cactaacccg tcggttggct gaagcgcgat ggtgcggacc
    14281 cgcccctccc cgatgtgctg ggccacttcc acgacgatat cgatcggcgc ggggacttcg
    14341 aaaccctcgc tggtgatgcg gatggccgtg ttgataacgg gaatttgccc ttcgggaaat
    14401 tgaatatcga cggctggtcc ggctacctga atcacccgcc cgattacggt tggaactgct
    14461 tgagttgcca taaaaaatca ctccagagcg gcagcgccgc tgacaatctc gataatctct
    14521 ctggtgatgc tcgcctgccg cacacgattc atgtacaaag tcaggttctc aatcacttcg
    14581 gccgcattcg aactcgccgc atccatggcg gtcattcgcg ccgcgtgttc ggcggcggag
    14641 gattcgagca tcgcctgata caccgcgatg tgcgcgtacc ggggcagcag cgcgttcaga
    14701 agttcctccg gcggctgctc atagatatag tcgattggat tctcgcgttc cgggatttcg
    14761 accggcaata tcttcaggag cgtgagattc tgcgcgataa cgctcttgaa ttcgttgtag
    14821 agcaggtaca cggcatcgat ttcgttattc gaaaagcgct cagccagccg cgatgcgatc
    14881 tggcgtgcgt cttcatacaa aaccttgttg aagatgcctg gatactcatc ggtgatgggc
    14941 acggttcttc tgcgccagaa gtcacggcct ttgcggccga gcagaagaag ctcgacctgc
    15001 atcgtctcgc gttcggccag gaaagattgc gacgccttga tcagattgga attgaaagcc
    15061 cccgccaagc cgcggtcgcc cgtgagcaga accagttgga tgcgttgctc ggctgctggg
    15121 cgggccagca gcgggtgctt cggggtatct tccgccccgg caaccacggc cccggcgacg
    15181 tcctgcaaga gcttgcgaag actcgccgcg tagggacgcg ccgagatcac ccggtcctgc
    15241 gcgcggcgca acttcgccgc ggagaccatc ttcatggcct tggtgatctg ctcagtattg
    15301 cggaccgagc ggatgcggcg tcggatatca atcaggctgg gcatggatca caccgctcaa
    15361 gccgctttat gttcggcagt aaaccgttcc ttcagttcct tcaacaccgc gttgattttg
    15421 tcgcgaagct ggtcgtcgag cgtctttttc tcccggatct cctgaagcaa cggcccgtgc
    15481 gcgttgtcga caaaccggta caattcgtcc tcaaacgccc ggcaggactc aaccggaagg
    15541 tcatccagat aaccattgat ccccgcgaaa aggatcaaca cctgtttttc caccggcagc
    15601 ggcttgtact gtggctgctt gagaatttcg accaggcggc gtccgcggtt gagctgcgcc
    15661 tgcgaggctt tgtcgagatc ggagccgaac tgggcaaacg cggccaggtt gcggtactgc
    15721 gccaggtcaa gccgcaagct gccggctacc tgacgcatag ctttaatctg cgcgtttccc
    15781 cccacgcggc tcaccgaaag cccgacgttc acggcaggcc ggatgttcga attgaacatg
    15841 tccgcttcaa gaaagatctg cccatcggtg atagaaatca cattggtcgg aatgtaggcg
    15901 gaaatgtcgc cggcctgcgt ttcgatgaac ggcaacgccg tcagcgatcc gccgccattc
    15961 tccgcattca gtttcgccgc gcgctccagc agacggctgt gcaggtagaa cacgtctccc
    16021 ggaaacgctt cccgtccagg cggccgccgc agcagcagcg agatctcgcg ataggcagcg
    16081 gcgtgcttgg agagatcgtc atagatgcac agcgcatgcc gcccggaatc gcggaaatat
    16141 tcgcccatcg agcagccgga atacggcgca aggtactgca tgggcgccgg atcggatgcc
    16201 gtggcggcaa ctacgatggt gtaatccata gcgccgtggt cctcgagcgt tttcaccacc
    16261 tgcgcaaccg tcgaccgctt ctgtccgatc gcgacgtaaa tgcagattac atcgccgccc
    16321 ttttggttga tgatggtgtc gagcgcaata gcggtcttgc cagtctggcg gtccccgatg
    16381 atcaactcgc gttgcccgcg cccgatcggg atcataccgt cgatggcctt aattccggtt
    16441 tgaagcggct ccttcaccgg actgcgatcc actacgcccg gagccagtct ttcaatggcg
    16501 ttgaattccg aggtctcaac cggccctttg ccgtcgattg gctctccgag tgcgttgact
    16561 acccgtccga caaggccatc tccgacagga accgacatga tccggccggt gcggcgcacc
    16621 tcgtcgcctt ccctgatctc ggtaaagtct cccagcagca cagcgccaac ctgatcttct
    16681 tccaggttca gcgcgaggcc ggaaacatgg tgcgggaact cgatcagctc cccggccatc
    16741 acccgttcga gaccatgaat gcgggcaatg ccatcgccca ctgatataac agaccccgtc
    16801 tcggagatat tgaccacccg gtcatagttc tcgatctctt cgcgcaggac gcgcgcaatt
    16861 tcatccgctc gcacttgagc catgctattt ttccaacctt ctcatgagag gcaaacgctc
    16921 gcctattcgg cgctaagccg cctgcgtagt gcttccaact gtccccgcac agagccgtcg
    16981 tagatggtcg acccgatacg cgccatcgcg cctccgagca gttccggatc gaccgcgaat
    17041 tccagtcgag ccgtgttgcc cgtgagcgcg gacaatctgt ctctcaaacc ttgacgctgc
    17101 cgatcgttca actcccgcgc cgaagcgacg cggacacgcg ttacgcccat tctctcgtcc
    17161 atgagcgcat caaacgcgtc gcggattgca tttagctccg ccacacgccg atgatcgacc
    17221 agcacaaaaa ggaaatttga aatcagcttg gagatggaca gatcgcttgc caggcggccg
    17281 atgacggccc gcttccggga actcgaaacg gcgggcgaga gcagaacgtt ttccaattca
    17341 ggggactcgg caagaacacg cgcgaacgag gtcaactgcg caactgcctc cgttggactg
    17401 acgctcgaag acgggcgggc taccaggtcc actaatgctt tggcgtagcg gccggcgacg
    17461 gcatgggcca tgcttattgt gcctccatcc ttgggcgttc aaggtccgca atgaaggagt
    17521 cgaccaaagc gtcttgcgta tcgggcgtca gccgagcacg aatcttgctc tcggcaaggc
    17581 gtaaggcgag ttcggccgag taggctttca actcctgacg ggacgccttg gccgccgctt
    17641 caatttcctg ctccgcgtgc gcctgcactt ttgacagttg ctgctccgtt tccatgcgga
    17701 cgcggccggc ttccgccgag atctcctggc gggcgttctc ccgcatgtgg tcgatctccg
    17761 tggccagcga cgccatccgt tgctcgagtt cccttgccct cgcctcggcg tcctgccgta
    17821 gacgggacgc ctccgcgatt ccactctgta tagacagtgt gcgcgcctgg aaaaagggga
    17881 ggccgaactt tttggtgaga tagccgaggc cggcggcaag cagggcgaag ttgacaatct
    17941 tccaaagcgt tatattgccc gtgtcgtgct cttcggctgc cgcatgttcc accgctttct
    18001 cactttctgt ttcatgagaa ggttcgggct gcgccaatcc agccgatacg aagaccagga
    18061 gcccgatcac caccatcgaa cagacgattt tcattgcacg ctcccggcga ggatgcgctc
    18121 gataatacga tccgcaaggg cttcgctttc agcggccagc cgctcgcggg aatcggctac
    18181 atcccgggcc aattgttcgc gcgcccgctc cacctgccgc tcggcttcgg ctcgcgcttc
    18241 gcgcacgcgc gcattctgtt cctcgcgcca atcgcggcgc attttttcct gttccgaata
    18301 gatctcggct ctcgcctggc gaatagcctg ttcgtactga gccgccattt cgccagcccg
    18361 tgcaaaactt gcttccgcgg cggcgcgcgc gccttccgta gctgcacgcc tggtttcgag
    18421 gagcttctcc aacggtccga agaacaccct cttcaggtaa aagtacagga atatcaccag
    18481 caacagagta ggaattcctc gtagaaggat cccaccgagc gcgttgagag tttgctccat
    18541 tgatgtaata ccgggagacc tgcgagaagc ttaagaaggc tccaaatccg ttaacctaac
    18601 acaggaaaaa agacccagtc aactggaatt cgctaagtca agctcttact gtgacttgac
    18661 acaatcgctc cccatcttat atcaagggtc tggcaaccca tgtggcggca aaactacatt
    18721 cctcttgcgg acagcctcgg gtggtccgct ctcgttgcgg cgcttccggt ctttgcgctc
    18781 ctctatctga ttggcgtacg caggaagcct gcctggatcg cggcgctggc gggcctgggc
    18841 gcggccgctg tagtagccct gttcctgtac agaatgccgt tcgggaccat gctgtccgcc
    18901 atcggatacg gcgcggcctt cggcctgttt cctattggtt ggattatctt ttgggcaatt
    18961 cttctttacc ggatcacctt ggaaacaggg aacttcgaaa tcctgaaaga ctctgtcggc
    19021 agcctgaccc atgaccgccg gctacaggcg ctgctgatcg ccttcgcttt tggagccttc
    19081 gtcgaagggg cggcgggatt cggtacgccg gtcgccgtgg ccgcggccat gcttaccggt
    19141 ctcggatttt cgccattcta cgcggcgggt atttgcctgc tcgccaacac ggctccggtg
    19201 gctttcggct cgatcggcat tccggtaatc accctggccg gcatcacggg cttgccgctt
    19261 ctcgagttga gtgcatgggt tggacgcatc tgtgcccccg tctccctctt cgtgcccgcc
    19321 tatctcatcc tggtgatggg cgggtggaag gcccttcgcg gtgtcgcgcc cgcggcgctt
    19381 ttgtgcggag cttcgtttgc acttacgcag ttcctggtct caaacttcat cgggccgcag
    19441 ttgaccgaca tcctcagctc gctcgccgcc attggtacgc tcgttctcct gttccggttc
    19501 tggcagcccc gggactcctt tcacctggag ggagaggacg ggcagaccaa atccgctgcg
    19561 ccgccgcgcc acggaaccgg caagacgctt ctcgcctgga ccccctacgg gttcctggtt
    19621 atcttcgtcc ttctctgggg catggattcg gtaaagacgt tgttgaacca tgtcacgttt
    19681 gccatcacat ggcccgggct gcacaacgtg gtttcgcgaa tcccgccggt cgtggcgtcc
    19741 gaacaggcat atcccgcggt cttcacgttc aactacctgt cggcggcggg cacggcctgt
    19801 atgttcgcgg cgctcgctac cgcgctgacg ctccggaccg gcatggggca gtttatacgc
    19861 attttcacgc acacggcgag gcaactggcg ctttcgatgg tgaccatcgc ggcagttctg
    19921 ggcctggctt ttctcatgaa ctactcgggc gctaccgcca cgctgggact cgctttcgcc
    19981 gccactggcg ccttgtttcc gttctttagc gcgatgctcg gatggctggg cgtgtttctc
    20041 accgggagcg atacgtcggc gaacgccctt ttcggaaatt tacaagtggt gacggccggc
    20101 agattgaatc tggaccccgt cctgatggcg tccgccaact cgagcggcgg cgtgatgggc
    20161 aagatgatca gccttcagag cattgccgtg gcggcggcag cgacgggaat ggcctcggcg
    20221 gatgaagcga aactattccg cttcaccttg cggcacagca ttattctcgc cacggccata
    20281 ggactcctca cgatggttta cgcatacgcc tttgggtagt tactttccgg ctcaaaaatg
    20341 cctttttcgc cggtcaacca ctctctcgaa agttactccg gaaaaatcac ggctgtgccc
    20401 ctttggcgtc cggcgaggtg atctggacgg tgtagccggc attccgaagt tcctgggtga
    20461 gcctttggat acggcgcttc atggcctgcg gggtgg
//
LOCUS       DASTMK010000532         7862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_90574, whole genome shotgun sequence.
ACCESSION   DASTMK010000532 DASTMK010000000
VERSION     DASTMK010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90574"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFQ79_22050"
     CDS             <1..540
                     /locus_tag="VFQ79_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEU0142420.1"
                     /translation="SPANISVDEQGAMHLRISRTEDDWSCAEASLTRNLGYGTYEFVV
                     RGLETLEPAAVFGMFTYDYASGALRNREMDIEISRWGDPANKNAQYALQPYDVATNVH
                     RFNVPAGTLTFSFRWEQGRITFRTLRGPARVSEHAFTAGVPSPGIESIRVALYVYRAA
                     VVKLQHPMEVVIERFTYYP"
     gene            515..3535
                     /locus_tag="VFQ79_22055"
     CDS             515..3535
                     /locus_tag="VFQ79_22055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEU0142421.1"
                     /translation="MSGSRTTREIRRALLLCTCAALSLHTLSALDPNRTVGQYIVTRW
                     GHDSFPGGAINAIAQTPDGYLWIGAENGLVRFDGISFRLIDHSSSPSLPPSPVLGLVV
                     DSEGVLWVRMQSPYLMRYRGGSFEQMYPAKVPPVFTFDREQGAAAVARGTSGDVLIAA
                     PGTPLRYAAGKFTPVVSLGAVHGIPISIAETADGAVWVGMRDTGLFCVRDGRGSQAGL
                     PDQKVNVLLPAAGSKLWIGTDSGLVHWDGGAITRRGVPAALVRSPILALARDRESNLW
                     ISTPAGITRMDSNGSTLQGTGGSSPGAVRAIFEDREGNLWFGGTEGLMQLRDAPFLSY
                     AGVAGEGGSLYVDTSGQAWVAPSSGGLLWIRGAEHQAITTLGMDRDVIYSIAGGPDEL
                     WAGRRLGGLTQLREEAGVLHMRTYTARDGLAPGAVYAVNRARDGSVWAGTLNGAVSRI
                     EKGRITTFTTANGLSADAVTTIQETPDGVIWVGTAGGLEAFRNGNWRRYGGEDGLPPG
                     RVNSLALDGDGVLWIGSSSGLFYWSGSRFESARSAPDSLRGEIYGLAADDSGNLWAAT
                     DRHVVSISRASLLGLSKDPAAARQFGRADGLPSTRGIRRDHSVIKDSSGRIWFSLQGG
                     LCVVNPSLLSALAPALVKVESVTVDGSPLGTEPAARYPSNRQRVVFNFIGLSLAVPGR
                     VRYRYLLDGYDSDWSQPTESREAAYTNLPPARYTFRVMASNSEGLWNGTPASVALEVE
                     PQLSETWWFRVIGLCLIAAVIFAGIRYRLARAHAAMNLRFEERLAERSRIARELHDTL
                     LQSFQGLMLRLQVVDDLLPPGKAKEQLEQSLERADQAIAEGRSAVYDLRSPATTKNDL
                     AEALRALGDELATQDSTAFHLVVEGPTRDLHPIIRDELYRITREALRNAFKHAAAQHI
                     EAEMIYGERAFRLRIRDDGQGIPPEMLEAGRPGHYGLSGMRERARQIGAKLEIWSGAK
                     AGTEIELSIAGSVAYHTSTGRPLFRLFRKKAGCL"
     gene            3541..4146
                     /locus_tag="VFQ79_22060"
     CDS             3541..4146
                     /locus_tag="VFQ79_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0142422.1"
                     /translation="MLSVDDHPLLREGIAALIGNQTDMQLVAEASNGREALELFRKHR
                     PDITLMDLQMPDMSGIDAMSSIRGEFPDARLIVLTTHAGDVQISRALKAGARAYLLKG
                     SLRKELLETIRAVYAGQKRLSPEAAAEIAEHAADDVLTPREVEVLRLVAGGNANKEIA
                     AQLSLTEETVKSHVRNILAKLGANDRTHAVAIGLKRGIIDL"
     gene            4229..4621
                     /locus_tag="VFQ79_22065"
     CDS             4229..4621
                     /locus_tag="VFQ79_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0142423.1"
                     /translation="MGCILQRLFSTFPNSWPGVGLLLLRLCLGIALLNFASETTFFTQ
                     ELIATVGGIFLIAGLWTPAMGALVALDEVWIALTSYSLPREDTWIHIFLAILSVSVAM
                     LGPGAWSIDARLFGRKRFDIDRTKSRNR"
     gene            4779..5912
                     /locus_tag="VFQ79_22070"
     CDS             4779..5912
                     /locus_tag="VFQ79_22070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0142424.1"
                     /translation="MVSVDLIGSSANFRALPTVLIQRETRTGTLLESELLGYESKNTY
                     LHDELRKEHNFEEILGNSPELLKLLDRVQSTAPTDANVLIMGETGSGKELIARAIHSR
                     SARRNSPLVKVNCGAIPAGLVESELFGHVKGAFTSASERRIGRFELANHGTLFLDEVG
                     ELPLETQVKLLRVLQEQEFEPVGSNRTIKVNVRIIAATNRDLEKAVQSGGFRSDLYYR
                     LNVIPLRVPALRERRSDIPEMVMVFLQQFAKRMGKPLQSVSRETMRLLVDYSWPGNIR
                     ELQNVIERGVVLSKGSILKLGADLLPVEASGTDIEPGTIAETKDSDSLEEVQRQHILR
                     VLERTGWVISGPRGAGAILDIHPNTLRSLMNRLGIRRALQAAS"
     gene            complement(5865..>7862)
                     /locus_tag="VFQ79_22075"
     CDS             complement(5865..>7862)
                     /locus_tag="VFQ79_22075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0142425.1"
                     /translation="GPNYLVMEYVEGTPLSGPSAVEVVLPYALQICDALAAAHDKGIT
                     HRDLKPANILLAASGIKLLDFGLAKVKVPGESDQTATHTQTGTILGTPSYMSPEQAQG
                     QSVDARSDIFSFGSLLYEWLTGRRAFEGTSALSTLASILRDEPAPLESAPEIQRVIAR
                     CLRKHPADRYQSVTELRAALEDATYHHAHTYPEHVPSIAVLPFANLSADKENEYFSDG
                     LADEILNTLTRLRGLRVTGRVSSFSFRGRENAITEIGHRLRVANVLSGSVQRSGNRIR
                     VSAQLINVTDESQVWSQHYDREMRDLFDVQDEIAQAIVAQLKVRLGSKSSQPLIKHYT
                     GNPKAHSLYLKGNFHYYKLTPGEMDKGRKLLEQAVELDPSHAPAWFSLADAYIAGAHF
                     GGVSPRQQWPKARAAAARALEADPESAEAKAAVGFVTAVSEFRWDEGLGELDAALRLN
                     PASARALFWRADVLHCMGRAEEAYADACRAAELDPLFTLYRRYCAYYCLLMGQPERAV
                     EHVHQLLEIDPNYIVGAYLLGEAYSLLGRHEEGIALLEKEQRDGPGAFLPLGFLAWAY
                     VRGGRRADAERFLTKLRVAARYRYVPPATIAMAALAVDDAETALLAMEEGVRERDPNL
                     SFGIRTHYFHRLRADARYHDILERMNLRSGLKGAPNSQSVH"
BASE COUNT         1582 a         2304 c         2384 g         1592 t
ORIGIN      
        1 tcgccggcaa atatctcggt cgacgaacaa ggcgcaatgc atttgcgcat ctccaggacg
       61 gaagacgatt ggagttgcgc ggaagccagc ctgacgcgga atcttggcta cggcacctac
      121 gaattcgtcg tgcgaggact ggagacgctg gagcctgccg cggtcttcgg aatgttcacc
      181 tacgattatg cgagtggcgc gcttcgtaac cgcgaaatgg acatcgagat cagccgatgg
      241 ggcgacccgg cgaacaaaaa cgcccagtat gcgttgcagc cctatgatgt cgccaccaac
      301 gtgcaccggt tcaatgtacc cgcagggacg ctcacgttct cgttccgctg ggagcaaggc
      361 cgcatcacct ttcgtactct acgcgggccg gcccgtgttt cggagcacgc cttcacggca
      421 ggcgtaccat ctccgggaat tgaatccatc cgtgtcgcgc tctatgttta ccgtgcagcc
      481 gttgtaaagc tgcagcatcc gatggaggtc gtaattgagc ggttcacgta ctacccgtga
      541 gatccgccgg gcgctactgc tttgcacctg cgccgcgtta agtctacata ctctctccgc
      601 cctcgacccg aatcgtaccg ttggccaata catagttact cgctggggcc acgactcgtt
      661 tcccggcggc gcaatcaatg ccatcgctca gactccggat ggctacctct ggatcggcgc
      721 cgagaacggg ctggtgcgtt tcgacgggat cagtttccgc ctgatcgatc actccagctc
      781 accctcgctt cctcccagcc ccgtgcttgg cctggtcgtg gattctgaag gcgtgctgtg
      841 ggtacgcatg caaagtcctt acctgatgcg ctatcgcggc gggagctttg agcagatgta
      901 tcccgcgaaa gttccgccag tgtttacctt cgaccgcgag cagggtgcgg ccgccgtcgc
      961 tcgcggaact tccggcgatg tgctgatcgc cgcgcctggt acacctctgc gctatgccgc
     1021 cggcaaattc acaccggtcg tttctttggg cgcggtccat ggtattccga tatcgattgc
     1081 ggaaacggcg gatggcgccg tctgggtcgg gatgcgcgat accgggctgt tctgtgtgcg
     1141 agacggccgt gggtcgcagg ccggcttgcc ggaccagaag gtgaatgtgt tgctcccggc
     1201 cgccggttca aagctgtgga ttggaacgga ctcgggcctg gtgcattggg acggcggcgc
     1261 catcacgcgt cgaggtgttc ccgcggcact cgtgcgcagt ccgattctgg ctttggcgcg
     1321 cgaccgcgag tccaacctct ggatctcgac tccggccgga atcacacgca tggattcgaa
     1381 tggctccact cttcagggga ccggtggaag ttcgcccggc gcggtgcgtg ccatttttga
     1441 agaccgggag ggcaacctgt ggttcggagg aactgagggg ctgatgcagc ttcgcgacgc
     1501 tccgttcctg agttatgccg gcgtggccgg tgagggtggg tcattgtatg tcgatacttc
     1561 cgggcaggct tgggttgcgc cctcctccgg aggcctgctc tggattcgcg gagcggaaca
     1621 tcaagcaatc accacgctgg gcatggatcg cgatgtgatc tactccatcg ccggcggacc
     1681 ggacgaactt tgggccggtc gcaggcttgg cggcctcacg caattgcgag aagaagccgg
     1741 ggttcttcac atgcgaacgt ataccgcacg tgacggcctt gcaccgggag ccgtttacgc
     1801 cgtaaatcgg gctcgcgatg gatccgtttg ggccgggact ctgaatggcg cggtgagccg
     1861 tatcgagaaa ggccgtataa cgactttcac aaccgccaac ggattgagtg cggatgccgt
     1921 aaccaccata caagaaacgc ccgacggagt gatctgggtg ggaaccgctg gaggactgga
     1981 agcgttccgc aatgggaatt ggcggaggta cggtggcgag gacggattgc ctcccggacg
     2041 tgtgaacagt ctcgctctcg atggcgacgg agtactgtgg atcggctcct cctcgggcct
     2101 gttttattgg tcgggctcgc gctttgaaag cgcacgaagc gcccccgatt cgctccgcgg
     2161 agagatctat ggcttggcgg cagacgactc cggcaacctg tgggcggcta cggaccgcca
     2221 cgtcgtcagc atatcgcgcg cctcgctact gggtctgtca aaggacccgg ccgcggcgcg
     2281 ccaattcggc agggcagacg gtctgccttc aacgcgagga atccggcgtg atcattcggt
     2341 catcaaagat tcgtcggggc ggatctggtt ctcccttcag ggcggattat gcgttgtgaa
     2401 tccgtcgctc ctctccgcac tggcgcccgc attagtgaag gtggaatctg tcacggtaga
     2461 tggcagtccg ctaggtacgg aaccagccgc ccgttaccct tcgaaccgcc agcgcgtggt
     2521 tttcaacttc atcggcctga gcctcgcggt tccggggcgt gtacggtatc gctacctgtt
     2581 ggacggctac gatagcgatt ggagccagcc aacggaatcc cgagaggcag cgtacacgaa
     2641 cctccctccc gctcgctaca cctttcgcgt catggccagc aacagcgagg ggctgtggaa
     2701 cggaacgcct gcgagtgtag cgttggaggt ggagccgcag ctatcggaaa cctggtggtt
     2761 ccgagtgatt ggattgtgcc tcatcgccgc ggtgattttc gccggcatcc gctaccgcct
     2821 ggctcgcgcc cacgccgcga tgaacctgcg cttcgaggaa cgcctggcgg aaagaagccg
     2881 catcgcgcgc gaattgcatg acacgctctt gcagagcttc cagggcctga tgcttcgcct
     2941 tcaagtggtc gacgatctgc tgccgccagg gaaggccaag gagcaactcg aacagagcct
     3001 cgagcgggct gaccaggcga ttgcagaggg gcgcagcgcc gtgtatgact taagatcacc
     3061 cgccacaacc aagaatgacc tggcagaagc gctgagggcg ctaggtgacg aattggccac
     3121 gcaagattcg acggcgttcc acttagtggt ggaaggccca acgcgagact tgcacccgat
     3181 cattcgggac gagctttacc gcatcactcg cgaagcactc cgtaacgctt tcaaacacgc
     3241 cgcagcccag cacattgaag ctgaaatgat ctacggcgaa cgggcattcc ggctgcgcat
     3301 ccgggacgac ggacagggta ttccacccga gatgttggaa gcaggccgtc ccggccacta
     3361 cggcctctcg ggaatgcgcg aacgcgcccg acaaatcggt gccaagctgg agatctggag
     3421 tggagctaaa gcgggtacgg aaattgaatt gagcatcgca ggttcggttg cctatcatac
     3481 gtcgacaggc cggcctcttt ttcgtttgtt ccggaagaaa gcgggttgcc tgtgattcga
     3541 gtgctttcag tagatgacca tccgcttctg cgcgaaggca tcgccgcgct gattgggaat
     3601 cagacagata tgcaactcgt cgcggaagct tccaatggac gcgaggcgct cgaactgttc
     3661 cgaaagcatc gtcccgacat cacgttgatg gacctgcaaa tgccagatat gagcggcatc
     3721 gatgcgatga gttccattcg tggcgagttt cctgacgcac gtctcatcgt tttgacaact
     3781 cacgccggcg atgtccagat ttctcgtgct ctcaaggcgg gcgcacgggc ttacttgctg
     3841 aagggctctc tgcggaaaga actgctggag acgattcgcg ctgtctacgc cggccaaaag
     3901 cgtctttcac cggaggccgc tgctgaaata gccgagcacg cggccgatga cgtcttgact
     3961 ccaagagaag tcgaggtact gcgtttggtt gcaggcggca acgcaaacaa agaaattgcg
     4021 gcacagctat cgctcaccga agaaaccgtg aagagccacg tccggaatat ccttgccaaa
     4081 ctgggagcta acgaccggac ccatgccgtt gcgataggtc tcaaacgcgg aatcatcgat
     4141 ctgtagttgc acgacatccc ccgaaagagg gacgccagcg aggcctttcg agggtgtcgg
     4201 accccggcgc gaacgcaata cgattgagtt gggatgtatt ctgcaacgtc tattctcaac
     4261 ctttccgaat agctggcccg gcgttggcct gctcctgctt cggctttgcc tcggcatcgc
     4321 cctgctcaat tttgcttcag aaacgacctt tttcacacag gaattgatag ccaccgtagg
     4381 cggtatcttt ctgattgccg gtctttggac ccccgcaatg ggggcgctgg ttgcactcga
     4441 cgaggtctgg atcgcactca cgagctactc tctaccgcgc gaggacactt ggattcatat
     4501 cttcctggcg atcttgtccg ttagcgtggc gatgctcggt cctggggcat ggtcgatcga
     4561 tgctcgtctg ttcgggcgga agcgctttga tatcgatcga accaaaagca gaaaccggta
     4621 actggcgaat ccccctaatg gggtagaaga gactcaaacc atcaaggggc ctgatcgaga
     4681 tcgatttggt gacgatcctc gaagtgagaa gagtgagaat gtgaaacaga agagccgatc
     4741 ctactgggtt cgatgccgtt caagcaagga gaatgacgat ggttagcgta gatttgattg
     4801 ggtccagcgc caatttccgg gcgttgccaa ccgtcctgat ccaacgtgaa accaggaccg
     4861 gcactctgct tgagagcgaa ctccttggct acgaaagcaa aaacacatac ctgcacgatg
     4921 aactccgcaa agagcacaac ttcgaggaga tccttgggaa cagtccggag cttctcaaac
     4981 tgcttgaccg cgttcaatcc accgcgccga ccgatgcgaa cgtcctgatc atgggtgaga
     5041 cgggcagtgg aaaagaactc atcgcacggg cgattcatag ccggagtgca cgccggaaca
     5101 gccctctggt gaaagtgaac tgtggcgcca ttccagccgg cctggtggag agtgagcttt
     5161 tcggacatgt aaagggcgct ttcaccagtg catccgagcg ccgcatcggg cgattcgaac
     5221 tagccaacca cggcaccctg ttcctggatg aagtgggtga gctaccgctg gagactcagg
     5281 ttaagttgct ccgggttttg caggagcagg agttcgagcc cgtcggaagc aaccgcacca
     5341 tcaaagtcaa cgtccggatc atcgccgcta cgaaccgtga ccttgagaaa gccgttcaat
     5401 ccggagggtt ccgctcggat ctttattacc gcctgaatgt gataccgcta cgcgtgccgg
     5461 ccttgcggga gcgccggtcc gatataccgg aaatggtgat ggtgttccta cagcagttcg
     5521 cgaagcggat gggcaagccg ttgcaatcgg tttcgcggga gaccatgagg ttgttggtcg
     5581 attactcctg gcctggcaac atccgcgaac ttcaaaacgt tatcgagcgt ggcgtcgttc
     5641 tctccaaggg ctcgattctc aaactcggag cggatctcct gccggttgag gcatccggga
     5701 cggacataga accgggaacg atcgctgaga cgaaagacag cgattcgctc gaggaagtac
     5761 aacgccagca cattctgcga gtactcgaaa gaaccggttg ggtgataagc ggaccccgag
     5821 gcgcgggagc catccttgat attcacccga acacccttcg cagcttaatg aacagactgg
     5881 gaattcggcg cgcccttcaa gccgcttcgt aaattcatgc gctccaggat gtcatgatac
     5941 cgggcgtcgg ctcgcagcct gtggaagtag tgagtgcgga ttccgaagct cagattgggg
     6001 tcgcgttcac gaaccccttc ctccattgcc agcaaagccg tttctgcgtc gtctacggcg
     6061 agcgccgcca tggcgatggt tgcgggtggg acataccggt agcgcgccgc gacgcgcaac
     6121 ttcgtcagga atcgttccgc atctgcgcgg cggccaccgc ggacgtaggc ccacgccaga
     6181 aagccgagcg gcaggaaagc tccaggcccg tcccgctgtt ccttttcgag cagcgcgatg
     6241 ccctcttcat gccgtcccag tagcgagtac gcttctccca gcaggtacgc gccaacgata
     6301 tagttgggat cgatctccaa gagctggtgc acatgctcca ccgcacgctc gggttgaccc
     6361 atgagcagac agtagtaggc gcaataccgg cggtagagcg taaacagcgg atcaagctct
     6421 gccgccctgc aggcatctgc gtacgcctct tcggctcgcc ccatgcagtg cagcacatcg
     6481 gcgcgccaga acaacgcccg cgcggaggcc ggattgagcc tcagtgccgc gtccagctcg
     6541 cccaggccct cgtcccacct gaactcactc accgctgtga caaatcccac ggccgccttc
     6601 gcttcggcag attccggatc cgcctccaga gccctggcgg ccgcggcacg cgctttcggc
     6661 cactgctgtc gaggagaaac cccgccgaag tgagccccgg caatgtaggc gtcagccaga
     6721 ctgaaccagg ccggcgcatg gctcggatcc aattcgaccg cttgctccag gagtttccgg
     6781 cccttatcca tttcccccgg cgtcaacttg tagtagtgaa aattaccctt cagatacaaa
     6841 ctgtgcgctt tgggatttcc ggtgtagtgc ttgatcagcg gttggctgga cttgcttccc
     6901 aacctgacct tgagttgcgc aacaatcgcc tgtgcaattt catcctgaac gtcgaacagg
     6961 tcgcgcattt cgcggtcata gtgctgggac cagacctgcg attcgtcagt gacattgatg
     7021 agttgcgcac ttaccctgat tcgattgccg gaccgttgaa cgctgccgga caagacattc
     7081 gcgacgcgga ggcggtgtcc gatttcagta atggcgttct cccggccccg gaagctgaag
     7141 gaagaaacac gaccggtgac acgcagaccg cggagtcgag tcaacgtatt cagaatctcg
     7201 tccgccaggc cgtcgctgaa gtactcgttc tctttgtcgg cgctcaagtt ggcgaagggc
     7261 agcacggcga tggaaggaac gtgttcgggg tatgtgtgcg catggtgata ggtggcgtcc
     7321 tccaacgccg ccctaagttc agtcaccgat tggtaacggt ccgcggggtg cttacgcagg
     7381 cagcgcgcga ttaccctctg tatctccgga gcgctttcca gaggggccgg ctcatcacgc
     7441 aggatggacg ccaaagtaga gagggcgctg gtaccctcaa aggctctgcg gccggtcaac
     7501 cattcgtaga gcaatgagcc gaaggagaag atgtcagacc gcgcgtccac cgattggcct
     7561 tgcgcctgct cgggcgacat gtaggaagga gtgccgagga tcgtcccggt ttgcgtatgc
     7621 gtagcagtct gatcgctctc gcccgggacc tttactttgg ccaatccaaa atccagcaat
     7681 ttgatgccag atgcagccag caggatattg gcgggcttca gatcccgatg cgtgatgccc
     7741 ttgtcgtgcg ctgcggcaag cgcatcgcaa atctgcaggg catacggcaa taccacctcg
     7801 accgcggaag gtccacttag cggcgtgcct tcgacatact ccatcaccag gtagttcggg
     7861 cc
//
LOCUS       DASTMK010000533         5727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_187082, whole genome shotgun sequence.
ACCESSION   DASTMK010000533 DASTMK010000000
VERSION     DASTMK010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187082"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1479)
                     /locus_tag="VFQ79_22080"
     CDS             complement(136..1479)
                     /locus_tag="VFQ79_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142426.1"
                     /translation="MNRRRFLSTGAAGISAAAHALPFAADKPRRVGLIGCGWYGKCDL
                     FRLIQVAPVEVVSLCDVDKRMLERAADLVAGRQKSGKKPRTYSDYRELLKQKDVDIVL
                     IATPDHWHALSMIAAVQAGADVYVQKPISVDIAEGQAMLAAARKYKRVVQVGTQRRST
                     PHLIEARENIIKPGKLGKVALVEIYCYYHMRARGNPPDTAPPDYLDYEMWTGPAPMRP
                     YNELVHPGRWRAFMEYGNGIVGDMCIHMLDMVRWMLDLGWPVSVSSSGGILIDKASKA
                     NISDTQTATFDFGDLKVVWQHRTWGTAPDPQYPWGATLYGDKGTLKASVMSYDYTPVE
                     KGAAAIHRDVKYELEEYPEDNTEERLEKHVAPAIRGHMRDFLAAIDARSKPVADIEQG
                     HISTTSCILANLSMKLGRTLAWDQAAGRIANDEQANRLLRRPYRQPWVHPDPARV"
     gene            complement(1551..2435)
                     /locus_tag="VFQ79_22085"
     CDS             complement(1551..2435)
                     /locus_tag="VFQ79_22085"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0142427.1"
                     /translation="MYSAVNNPLVWMVILMGWYGPGVEAGDIFERVWRGTGTTRNVAG
                     PLTVLSWNIERGLNLPGIEAVLEQHAPDVALLQEVDLNSRRTGRRNIAEDLARRFHMH
                     WAFAVEFEELGQGDSSSPAYVGQAVLSCLPIRSARILRFKEQSDFWQPRWFIPDWGIF
                     QRRRGGRMALIAELVSKEGPLIVYDTHLESRGPEKRRLRQIDEIIADVAKHAPTAPVV
                     IAGDFNTRLRPSPVIDRLREAGFRQAAGSDKSTSARGAALDWIFVRGPLTFREGSVLS
                     DVEASDHYPLKVQLSIKR"
     gene            complement(2441..3382)
                     /locus_tag="VFQ79_22090"
     CDS             complement(2441..3382)
                     /locus_tag="VFQ79_22090"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0142428.1"
                     /translation="MAIAKRRLGRTNLMVSAISLGTVELGMRYGIAPVGQDEKPGEEQ
                     AARLLNRALDLGVNYIDTARAYGDSEAIIGRSIAARREEFVLASKVPPFHLEGLTPKE
                     LRKRAGIAVEDSLRALKTDVIDIMMIHCAPDREFGHEVLLDTLDAGRMAGRIRFIGAS
                     VYGEDAALKAILSGRCDVIQVAYSVLDRRPESRVLPAAMAHDVGIVARSVLLKGALTY
                     RASQLPDSLAELKDAAMRMEAAADSMAGGLPELAYRYVLAHQDVHTALVGTGRIEELE
                     AAVGYAGRGELPAETLARIREVAVRDERLLNPGNWSL"
     gene            3537..4322
                     /locus_tag="VFQ79_22095"
     CDS             3537..4322
                     /locus_tag="VFQ79_22095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0142429.1"
                     /translation="MIPALDLFRLDGKVALVTGGAGIYGSHIARALAEAGATVVVASR
                     DAAKCQAFADKLRREDLAVEAQRCDLASENDIRELRDWLTGRYGKLDVLINNAVARGG
                     GDLHHVTAEQWESAMKVNSTGLMLACRIFSEPMQAQRSGSIVNISSIYGMVGPDFSIY
                     EGTSMMNPADYAFAKGGMINFTRYLASFLAPFNIRVNCLSPGGFETEGMPESFLRNYK
                     RKTLLRRMAEADDIKGPVIFLASEASRYITGQNIAVDGGWTAV"
     gene            complement(4339..5139)
                     /locus_tag="VFQ79_22100"
     CDS             complement(4339..5139)
                     /locus_tag="VFQ79_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEU0142430.1"
                     /translation="MSLQRADPPIIIKKKKVVHGHHGGAWKVAYADFVTAMMALFIVL
                     WLMNTSKEVQDAISGYFRDPAGTGSSKGTGLGGQGSGLELGRQDLDQLKEKLEAAMRE
                     IPTLSALKDQVAMTVTGDGLRIELLEGEQGTFFESGNAHPSAAGTEMLAMLAVELGKL
                     PNRVLIEGHTDSRPFAGDRVYSNWELSTDRANSARRLMVEKGIRQGQVAEVRGYADQR
                     LRHPQEPHNPSNRRVSILVQPPAPGAETGGTDLAKAMPSHTADTHQSK"
     gene            complement(5103..>5727)
                     /locus_tag="VFQ79_22105"
     CDS             complement(5103..>5727)
                     /locus_tag="VFQ79_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU0142431.1"
                     /translation="LTALEKDVDQPDQSELFAKFPPGAPDHHIRDFVCDTWRMAITGG
                     PSPHEMDLMMELDMEVHHHYSHQPSAALTAVADALPGLGIVAAVLGVVITMGALGGPP
                     EEIGHKVAAALVGTFLGILLCYGLVGPMASRMLRTAEDEGHFYHVLRVAITSFLKGSS
                     PILAVEFARRTIPGHVRPSFHEIEGACRGAAGNSISCPFNEPIHRSS"
BASE COUNT         1106 a         1741 c         1698 g         1182 t
ORIGIN      
        1 ggcgtccagc aggattggtc aggtcgttcg cggccgtgct cacaatggca atggcaatag
       61 caacggcaac ggacacggcg gctccggaca gcgcaaggac gtgctgactc aattgcaacc
      121 gctggacccg gcgcgctaaa cacgcgcggg atcgggatgc acccacggct ggcggtacgg
      181 ccgccgcagc aaacggttag cctgctcgtc atttgcgatg cggcccgcgg cctgatccca
      241 ggccagagtc cggcccagct tcatcgacag gtttgccagg atgcagctcg tagttgaaat
      301 gtgtccctgc tcgatatcgg cgaccggttt cgagcgggca tcgatggcag ccagaaaatc
      361 gcgcatgtgc cccctgatgg ctggtgcgac gtgtttctcc agccgctctt cggtattgtc
      421 ttccggatat tcctcaagct cgtatttcac atcgcggtga atagcggcgg cgcccttctc
      481 caccggcgtg taatcgtagc tcatcacgct tgctttcagt gttcctttgt cgccataaag
      541 cgtcgcgccc caggggtatt gtggatccgg cgctgtaccc caggtgcggt gttgccagac
      601 gactttcaga tcgccgaaat cgaaagtcgc agtctgcgtg tcggagatat tggctttgct
      661 ggccttgtcg atgaggatgc caccggaaga agagacgctt accggccagc cgagatcgag
      721 catccagcgc accatgtcga gcatgtggat gcacatgtcg ccaacgattc cgttgccgta
      781 ttccatgaac gcccgccacc gccccggatg aaccagttcg ttgtaagggc gcatgggagc
      841 gggaccggtc cacatctcgt aatcgaggta atcgggcgga gcggtatccg gcggatttcc
      901 acgcgctctc atgtggtaat aacaatagat ttcgaccagg gcgacttttc ccagcttgcc
      961 gggcttgatg atgttctccc gggcttcgat gagatgcggt gtgcttcgcc gctgcgtacc
     1021 cacctgcacc actcgtttgt atttacgggc ggccgcgagc attgcctggc cctcggcgat
     1081 gtccacgctg atcggcttct gaacgtaaac gtcggccccg gcctggacgg cggcaatcat
     1141 cgatagggca tgccagtgat ccggcgtcgc gatgaggacg atgtcgacat ctttctgctt
     1201 tagcaactcc cggtaatcgg agtaggttcg aggttttttt ccggacttct gccggccggc
     1261 cacaagatcc gccgcacgtt cgagcatgcg tttatccaca tcgcaaagag agacaacctc
     1321 gaccggtgct acctgaatca gccggaacag gtcacacttg ccataccagc cgcagccgat
     1381 cagcccgacc cgtcttggtt tatcggcggc aaagggaagc gcgtgtgcgg cggctgatat
     1441 cccggcggct cccgttgata aaaatcggcg gcggttcatg agtcgggttc ctcctcgaaa
     1501 agatatttgt tatggaacca gtatagacgt cgcaggtagc gtctccgcgg ctagcgttta
     1561 atggataact ggaccttgag cgggtagtgg tctgaagctt ccacatcgga aagcacgcta
     1621 ccttcgcgaa acgtgagggg accgcgaaca aagatccagt ccagagccgc gccacgggcg
     1681 cttgtgctct tgtcgctgcc ggcggcttgc cggaatccgg cttcccgcag gcggtcgatc
     1741 acaggcgagg ggcgcagccg cgtattgaaa tcgcccgcga tgacaaccgg tgcagtggga
     1801 gcgtgtttcg caacatcggc gatgatctcg tcgatctgcc gcagccgccg tttctccgga
     1861 ccgcggcttt cgaggtgagt gtcgtagacg atcagcggtc cttcctttga gaccagctcg
     1921 gcgatcagcg ccattcggcc gccgcggcgc cgctggaaga tgccccagtc cgggatgaac
     1981 cagcgtggct gccagaagtc cgattgttct ttgaagcgga ggatgcgggc ggagcggatt
     2041 ggaagacagc tcaacacagc ctgtcccaca tacgccggtg aggagctgtc accttgtccg
     2101 agttcctcaa actccaccgc aaaggcccag tgcatatgaa accgccgtgc cagatcttcg
     2161 gcgatgttcc tccggcctgt gcgccgggaa ttcaggtcga cttcctgcaa aagcgccacg
     2221 tccggcgcat gctgctccag gaccgcttcg attcccggca gattcaaccc gcgttcgata
     2281 ttccaactca acaccgtcaa cggacctgcg acattccgcg tggtacccgt tccacgccat
     2341 acgcgctcga aaatatcgcc ggcttccacc cctggcccgt accagcccat caggataacc
     2401 atccatacca gggggttgtt cacagcagaa tacatggatc tcacaacgac cagtttcccg
     2461 gattcagtag gcgttcgtcc cgaaccgcga cttcccggat gcgcgctaac gtctcggcgg
     2521 gcaactcgcc tcgtccggca tagcccacgg ctgcttccag ttcctcgatg cggcccgttc
     2581 ccaccaaagc cgtatgcacg tcctgatgag ccagcacgta ccggtaagcc agttccggca
     2641 gtccccctgc catgctgtcc gccgccgctt ccatgcgcat cgccgcatct ttcagttcgg
     2701 cgagtgagtc cggcaactgc gacgcgcggt atgtaagcgc gcccttcagg agtaccgacc
     2761 gggccacgat gcccacatcg tgcgccatcg cagccggcag tacgcggctc tccggccggc
     2821 ggtccagcac gctgtacgcg acctgaatca cgtcgcaacg gccggaaaga atcgccttca
     2881 acgcggcatc ctcgccgtac acgctcgcac cgatgaatcg aatccggccg gccatcctcc
     2941 cggcatcgag tgtatcgagc aggacttcgt ggccgaactc acgatccggg gcacagtgaa
     3001 tcatcatgat gtcaatgaca tctgttttga gcgctcgcag gctatcctcc accgcgatgc
     3061 cggcgcgttt ccgcagctct ttcggagtaa ggccctccag atgaaacggc gggaccttcg
     3121 atgccaggac aaattcctcg cggcgggcag caatgctgcg cccgatgata gcctcactgt
     3181 cgccgtaagc ccgcgccgtg tcgatgtaat tgacaccgag atcgagcgcc ctgttgagca
     3241 aacgtgcggc ttgctcttca ccgggcttct catcctgacc aaccggtgcg atgccgtagc
     3301 gcattcccag ttcgacggtt cccagagaaa tcgcggagac catcaggttg gttcgcccca
     3361 aacgccgttt tgcgatcgcc atgtgatccc cgtataacaa tttcgtctct tcagggcgcc
     3421 tatttttaca tatttcggat cggaggcgcc gcgcctcccc attctccggg tggcgggttt
     3481 tggtgcgaaa gtgggtgagg aggtgcggtg gagcggataa gtgtgagagc gcgagaatga
     3541 ttccggcgct cgatcttttc aggctggacg gcaaagttgc gctggtcacg ggcggcgctg
     3601 gtatctatgg ctcgcacatt gcaagggccc ttgcggaggc cggcgcaacg gtggtcgtgg
     3661 cgtcgcgcga cgccgctaaa tgccaggcct ttgcggataa gcttcgacgc gaagacctgg
     3721 cagtggaagc acaacgctgc gaccttgcct cggagaacga tattcgcgag cttcgggact
     3781 ggctgaccgg ccgctacgga aagctggatg tgttgatcaa taacgccgtg gcccgaggtg
     3841 gcggggatct gcatcacgtt acggcggagc agtgggaatc cgcgatgaag gtgaactcaa
     3901 ccggactgat gctggcgtgc cgtattttct cagagcctat gcaggcgcag cgatccgggt
     3961 ccatcgtcaa catctcttcg atttacggaa tggtggggcc ggatttctcg atctatgaag
     4021 gaaccagcat gatgaacccc gccgattatg cattcgccaa gggcggcatg atcaatttca
     4081 cccgttacct ggcgagtttc ctcgcgccat tcaacattag ggtgaactgc ttgtctcccg
     4141 gtggattcga aaccgaaggg atgccggaga gctttctgcg gaactacaag cggaagacgt
     4201 tgttgcggcg tatggcggaa gcggacgaca tcaaaggtcc cgtcattttc cttgcttccg
     4261 aagcatcccg ctacattacc ggacagaata tcgcggtgga cggcggctgg acggccgtgt
     4321 gagcagaagc cggacgccct atttcgattg gtgggtgtcc gccgtgtggg acggcatggc
     4381 cttggcgaga tctgtaccgc ccgtctctgc acctggagcg ggcggctgta ccagaatgga
     4441 gactcttcga ttcgacggat tgtgcggttc ttgaggatgg cgcaaccgct ggtcggcgta
     4501 gccgcgcacc tctgccacct gtccttggcg aatgcctttt tccaccatca aacggcgtgc
     4561 ggaattggcc cgatctgtcg aaagctccca attggaatag acccggtctc ctgcaaaggg
     4621 ccgggagtcc gtatgacctt caatcagaac gcggttcggc agctttccga gttccacggc
     4681 cagcattgcc agcatctcgg taccggcggc gcttggatga gcattcccgc tttcaaagaa
     4741 tgtgccttgt tcgccttcga gcaactcgat gcgtaaaccg tcgccggtca ccgtcatcgc
     4801 cacctggtcc ttcaaagcgg acagcgtcgg gatttcgcgc atagcggctt cgagcttctc
     4861 tttaagctgg tcgagatcct ggcggccaag ttccagtccg ctgccttgcc ctcccaagcc
     4921 cgtgcctttg gaggagccgg tgcctgcagg atcgcgaaaa tatccggaaa tcgcatcctg
     4981 cacttccttg ctcgtgttca tcagccagag cacgatgaac aaagccatca tcgcggttac
     5041 gaaatcggca taggctactt tccaggcgcc gccgtgatgg ccatgaacca ccttcttctt
     5101 ctttatgatg atcggtggat cggctcgttg aagggacatg aaatgctatt tcccgccgct
     5161 ccgcggcagg cgccttcgat ctcgtggaaa gatggacgaa catgacctgg gatcgtgcgc
     5221 cgggcaaact cgaccgccag gatcggcgag gagcccttca gaaaggaagt gatggcgact
     5281 cgcaatacgt gatagaaatg tccttcgtcc tcagccgtgc gcagcatgcg ggaagccatc
     5341 gggcccacca gtccatagca cagcagaata ccgaggaagg tgccgacgag cgccgccgct
     5401 accttgtggc cgatctcttc cggtgggccg cccaaagcac ccatggtgat gacaactccc
     5461 aggaccgccg ctacgatgcc cagccccggc agtgcgtcgg cgacagccgt gagcgctgcg
     5521 gaaggctgat gggagtagtg atgatgcacc tccatgtcga gttccatcat gaggtccatc
     5581 tcgtgcggtg acggtccgcc ggtaatcgcc atccgccatg tgtcgcagac gaagtcacgg
     5641 atatgatgat ctggagcgcc aggcggaaat ttcgcgaaca gctcgctttg gtccggctga
     5701 tccacgtctt tctccagcgc tgtaagc
//
LOCUS       DASTMK010000534        19454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_60429, whole genome shotgun sequence.
ACCESSION   DASTMK010000534 DASTMK010000000
VERSION     DASTMK010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19454
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60429"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFQ79_22110"
     CDS             complement(<1..247)
                     /locus_tag="VFQ79_22110"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0142432.1"
                     /translation="MHALSFALIVVVIATGSTNFVLAESLTRLERQRLVAHLEMTEQE
                     LSDAIADLSSAQLQFRPSAESWNILEVLEHLVVAEPIY"
     gene            342..2216
                     /locus_tag="VFQ79_22115"
     CDS             342..2216
                     /locus_tag="VFQ79_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142433.1"
                     /translation="MATQTLTETPTNTGSNGKNGCCNGIRIDKCGDVHIHNCAPPEYC
                     NKYPPEAAQGTCIPIVAGAKHKLSRQLKMTELADTVRVPSSIATSIVHMARRFVLGHQ
                     PANALETAAFPVLERISGEMLSCTVDAFDALTARQRKRLFADSLLMDPDQPISEEMLV
                     AALAEEIKGRAGMEIFGDPLAGEQERPGKIRVFPPSGEIPPSQVRICRVNGLRTANFV
                     PLPPIGDFLPSELHQDCSPIIVNGQPQVVCQVRTMNCPGSVIDSTVCARVLEVASGDS
                     LTLEGVNYFSTDATVRITDRETGATVRDVEAHVWGDVDTPVTEVVNGETVLINDCRVH
                     DRLTFRIPEDLSPGIYQIQVFVPNITGIPAFGDFLNSDGEFIQVVTPSTARFQMVTEK
                     IVARQETSPARFGSDEVGLQAMAFALFADGTFGDAQEEKFKDIRDVDFDSGTSRDITR
                     LVFSHDQPILGMALSFAGFEIDSERAFNELITSRMDFFTQLIKDQAKFIGAAITALGG
                     ASALTKLGTVGLWIAGIAAAVVLAINVIAALWAPADPIIRDSFGFSTTDLDRLTSASF
                     PAPPPSTFETEGDGEISVNVNKTVPPEKVPLQYRETREYVSGTEDSRYEITYRHNRIA
                     "
     gene            complement(2289..2909)
                     /locus_tag="VFQ79_22120"
     CDS             complement(2289..2909)
                     /locus_tag="VFQ79_22120"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEU0142434.1"
                     /translation="MSHHLLRLIAANAIVFSLPSAAMDQAAKMTVPSMPSELQVPAGN
                     TLYLKGYAVGTQNYVCLPGAGGPAWRFLGPQATLFLTIPWIQGEIRQQIATHFLSSNP
                     AEAGTARPVWQHSLDTSAVWGRVKASSTDPNFVAAGAIPWLLLEAVGAQRGPIGGSAL
                     VQTTYIQRLNTSGGVAPTSGCNEPAYGAVVLVPYTTDYFFYRDARR"
     gene            3058..3612
                     /locus_tag="VFQ79_22125"
     CDS             3058..3612
                     /locus_tag="VFQ79_22125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU0142435.1"
                     /translation="MPELIARLRTGDESALGELTPTLYGELRRIASRHLRGERSNHTL
                     QATALVHEAYLKLIGARERQFADEVHFLAVASRVMRQVLVDYARSRSTQKRAGGARVE
                     PLWTTTLEVKSDDGMELIELIELDQAIEALAAENEPLARLIEMRYFGGMTAEESAEAL
                     GLSVHVVRHDLRFAQAWLRRKLSP"
     gene            3702..6338
                     /locus_tag="VFQ79_22130"
     CDS             3702..6338
                     /locus_tag="VFQ79_22130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0142436.1"
                     /translation="MIGEEVRHWFEQAVNVTAGGRTAFLKSNCRDEAARSEVLSLLEY
                     DAGNTGPAPGLPVPEALFQEAIGTMLGKGNPVLPARRAGPFELGRLLGSGGMGFVYEG
                     HRVDGAVRQRVAVKFAQVLPGTSRELRESAHRRFYRERQMLASLRHPYIAGLIDASTT
                     ADGIPYAVIEQVDGVPIDSYCDTWHPDQAERIRLILKLCDAVQFAHRNLIVHSDIKPD
                     NVLVTADGIPKLIDFGVASDLAEEATLTAMRAFTPGYASPEQSRGLPATVATDVYGIG
                     AVLYRVLTGAKPRELKSGPLSEVVRCISEEDVIRPSSIKPELKGDLENILLKALQRDP
                     QRRYGSVPELADDLNRFLARRPVRASPDSMLYRASRFVRRHWVSLAAAATLILSLAIV
                     TIISVRQREHAMQRAMETRRLADRLLFEVHDEIAGVLGGTKAREKLGAIAVQYLEGLE
                     RDYGRDPELAWELLNAYSRLGQSRGGAASSVGDTRSGTHFAMKTLELGAVVEAAAPGT
                     DRLDKLFAVYESLVPIFQEAGLPGQQREAIDRMVHLAPHLQPLRQAQALKELGRYLDA
                     NGSVAQGAEAFERSLTILRTLSRSPSKPAGTEAQLISTLVGFGRAQALRGDFSGAVAS
                     LQEAIRLSGNSTASDPHRAKSARQLYWSHIALGDVFGSPLRFNMGRSDDAVEHYEKAR
                     SIAENLVKADPGNEAAKLDLARAFSRQGVALAASQPAQSLVLLERSHSLALETSYQNH
                     SGLDSRFAYLTSSVEPLVRLGYFERARLHIAEARRLLKELRQAGVRVNENNLLRADAI
                     RLYASGHAKEALAEAQKHLALVPETTSSVLSDNFERVELLERIRIYAAGIDATAYASA
                     TAQLARIWENLKTTVSRRNPAG"
     gene            complement(6315..7373)
                     /locus_tag="VFQ79_22135"
     CDS             complement(6315..7373)
                     /locus_tag="VFQ79_22135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0142437.1"
                     /translation="MLTRRSLLLGLAAAGGVPRNTVVFRAGDDGYHTFRIPALLTTRN
                     GTLLAFCEGRRDSSSDTGNIDLVLKRSKDGGITWSPLQLVADHGPDTIGNPCPVQDRK
                     TGTIWLPLTGNPGHISQKQILEGAAIRRMWLSRSADDGATWEKPVEISGSARDPSWTW
                     CATGPGNAIQLKSGRIVVPCDYAPTGTWREHSYVFYSDDHGRTWKRGGELPEGTGESQ
                     AAELPDGSLVLNCRSDRGNRRLVGRSKDGGMTWGEFGPDETLIEPGCQASLIRHEKWL
                     LFSNPAAEKRVRMTVRASSDGGRTWPHARLLHEGPAAYSSLAVLRDGAVGCLYECGES
                     RPYETITFAHFSTGWITS"
     gene            complement(7407..8066)
                     /locus_tag="VFQ79_22140"
     CDS             complement(7407..8066)
                     /locus_tag="VFQ79_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HEU0142438.1"
                     /translation="MHFEILIEDRSGELLLESLLPKILGAKGDLHTWRTHAYRGIGRV
                     PPDLRGKTDPWKRVLLNQLPRILGGYGKSLQNQDAAVVVVVDLDNRECIGFKEELLQT
                     LQNCHPRPRVLFRLVIEEAEAWLLGDRCAILKVFPRAKATVLNSYVPDSICGTWEVLA
                     DAVFPGGSAKLKSDGYPRAGEEKCRWADLVGPHIDVERNQSPSFQAFRSGMLKLATKG
                     K"
     gene            complement(8066..9292)
                     /locus_tag="VFQ79_22145"
     CDS             complement(8066..9292)
                     /locus_tag="VFQ79_22145"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0142439.1"
                     /translation="MALIEGFHVRNYRALKDITIGRLWNQQRTPPLTALVAVIGKNGV
                     GKSTLFDAFGFLSDCLAVGVEEACDLKQRGGFDRLVSSGVKEPIRFDVYYRESPGDRP
                     ITYELSISRDGSGRPFVADERLRQRRKGQSRGRPLSFLYLRAGQGEAWAGEESFEGEE
                     AGRIPVELTDSRRLGIATLGTLRSHPRIAKFRRFLESWYLSYFTPDAARSLPMAGPQK
                     HLNMHGDNLGNVVQFMEREHKQRFQSILNRIASKIPGIEKIDTKKTDDGRLLLRFNDR
                     GFQDPFFAQQMSDGTLKMFAYMLLLEDPDPAPFICIEEPENGLYHKLLETLASEFRSH
                     ATGRKNAPQIFVTTHQPYFVDALRPEETWVLEKGKDGFSVIRRASSDTTVQSMVEHGL
                     PLGSLWYSDYLDARSA"
     gene            9551..11524
                     /locus_tag="VFQ79_22150"
     CDS             9551..11524
                     /locus_tag="VFQ79_22150"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU0142440.1"
                     /translation="MLNRRVFLITPAALLHGAALDRRAVIQQRADALATFPGQRVEAS
                     RSEKADQIAAGTVFFYSRTPVKVGLRNIDWSGGHIKHQEWPAQLNRFFHLGPLASAYL
                     ATGDEVYARAARAYIEDWLREDPYPETRSFRPGDNALTMSIRLGTSVASGWSGTLPVF
                     LRSPAFDDQFLDRIFHSLARQAQFLAARLTAVGNWRISELDALVFIALRFPFLDGAPV
                     LLETGITGMRNALATQFLPDGVHIERTPGYHDWMTQVAVNYADLARRFPDADAQVDAA
                     RAVRALDYGAQSELFGVNDSIAPNLDPPSFSRLERRRNSLAVLHLADRTPPEPPLDQV
                     FPDAGQVFSRTAWKSGADYLAFDASTWGGGHSHLSRLSFAFRSGGRALVADPGILSYE
                     MSDPRAPYGKSTAAHTTLNVDGYNQSGASGQLLRTAFTPDIALIHARYEGGWWKGQYR
                     WNFGQGRGEGIWGEHQRILFWVKREYLVVIDSLTTDAGHEIRNCWQMGPVEKWAQDAE
                     GLRWWSANRDVNVLLQLAVAPKETTMQCFEGSNKPLRGWVGQHGDNSVAAPLVEFRYP
                     SVLNRPVVTAALLAPSRGAVPPSWRITAAGATSNVGHLEIVTPNGATDLLAWTPGLAL
                     PIEDSQPFVTDARLVWTRQGTGRRFLLDGTYLK"
     gene            complement(11545..12693)
                     /locus_tag="VFQ79_22155"
     CDS             complement(11545..12693)
                     /locus_tag="VFQ79_22155"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEU0142441.1"
                     /translation="MQLTPTVILLSISLAVTGCNRPGERAAGTAAVRQFGKTAKGEPV
                     QLYTLRNSKGVEASITDYGAILVSLKVPDRNGNATDVVLGFDSLDGYLGEHPYFGAVI
                     GRYGNRIAGGRFVLDDVEYKLARNNGENHLHGGIRGFDKAVWKLKEPRADGDDGLEFT
                     YLSEDGEEGYPGNLTATVRYTLTGDNELRLDYEATAGKPTVVNLTNHSYFNLAGAGNG
                     EILAHRISINADRFTPVNQGLIPTGELRAVADTPFDFRQPHAIGERIDGKDQQLVYGK
                     GYDHNFVLNGAAGSLRPAARVTEPGSGRVMEVLTTEPGVQFYTGNFLDGTIRGKGGTA
                     YGHRYGFCLETQHFPDSPNQPQFPSVVLRPGAKYQSTTVYKFSTEPAR"
     gene            12813..14723
                     /locus_tag="VFQ79_22160"
     CDS             12813..14723
                     /locus_tag="VFQ79_22160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0142442.1"
                     /translation="MGESKSKPSSDSSRPAVRRYLAALLLVTAASLLRLVLDPILAER
                     SPYLIFLSAAAAAAWYGGFGPGLLATVLGVFGALAFAVDPLLTFRVDTPGDWLALALY
                     LIFGVFVSALTEKLHRTKRRALDAEASERRQRLLAQQTLAGIGDAVLSTDALGRIEFM
                     NAIAEQLTGWTMDDARGRPVRQVFQIANEYTGAPADDPITRVLREGIIAGLANHTVLI
                     KRDGSRIPIDDSGAPIRDESGNLIGSVLVFRNIAERRRTEQERERLIRDLTETRDIIK
                     SVFRKAPVGLALWDLDLRFTRVNEALVEMTGLPMGAHIGKTIDELLPGLPPEVTEAMR
                     QVRETRTPFRHETDGETPASPGKRRSWAIHYYPIRVDENIAGIGAVFQEITEKKAWEA
                     ELELANDNLKQFAYAASHDLQEPLRMITSYTQLLKKRYYDKLDSDGREFLDFAMDGAN
                     RMRTLLEALRDYWRAGEDGGEPVTVDCNAALETALGNLRASLAETGAVVTHDDLPKVV
                     AHEVPLVQIFQNLIGNALKYQNEAQPRVHISASGHGAQCVFSVRDNGIGIDKRQLPKL
                     FGAFRRLHGSKYPGTGMGLALCHKIVERYGGKIWVDSESSKGSTFYFTLPLASAETAA
                     GRASRLGERPAT"
     gene            complement(14743..17046)
                     /locus_tag="VFQ79_22165"
     CDS             complement(14743..17046)
                     /locus_tag="VFQ79_22165"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEU0142443.1"
                     /translation="MVQEKSGLWKHGLLLTLLFGFTVMIVGGYSMYRSRAPIPEVTVG
                     PRGQTLFTVEDIQNGQDLFRKRNLMNYGSVLGHGAYLGPDYTAEALHIMTAGMQAAGS
                     GGAYETLDLGQKAAIDAEIASELRQNTYENGILRFTERQVAGWNRIVDHYERTFAKGD
                     TTRSLPKGALLPEREGGGNITLDRQAAKELAAFVTWTAWLSVAKRPEADHSYTNNWPY
                     DKTAGNTATAGSMMWSAASVAGLLMFLALILYLHQRYRLSPVDVENPRLRFDVARSPL
                     TPSQRAAAKFFVVAILLFLVQTLLGGKMAHDYAEGATFYGLDLSQYLPFNIARGWHLQ
                     LAIFWIATAWLGMGIFIAPLVSGREPKGQKHLVNILFWALVVVVVGSMTGEYLSVKGY
                     LGETWWWFGTQGWEYLELGRIWMYLLITGMLIWLYIVYRGLKAALQSEGDRGGLTHLL
                     LYSSAAIPIFYCFALFIDRNSHITMADYWRWWLIHLWVEGMFEVFAVVVIGFLMVRLG
                     LVTAQSTLRALYFQLIILLGSGIIGTGHHYYWIGAPESWMALGAIFSALEVIPLTLLM
                     VEAYGQYKVIRDGGIEFPYKAAFWFLVATAFWNLFGAGALGFLINLPFVSYFQHGSYL
                     TAAHGHGALMGVYGMLAIALAAFSLRNIVDPKYWKEKWIMTGFWGLNIGLMGMIVTTL
                     FPVGVMQALESFRNGFWSARSFEFYQQPLVNKLLWLRMVPDTVFIVAGVLPIAAAAVY
                     GLFHLRALRPIPAELPVEQEEGELVGV"
     gene            complement(17132..17812)
                     /locus_tag="VFQ79_22170"
     CDS             complement(17132..17812)
                     /locus_tag="VFQ79_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0142444.1"
                     /translation="MNENKAEIFRRIQLFAGLSTPELEAIASHAVEKRYRSGDVLFNE
                     GEECDGLYVIAQGAVKIYKTSMAGREMTIALEQAPGTVAELPMFDGGAYPASVAAIND
                     VRAILIPKREFSQVCRQYPDVALKVLAVVGKRLRHLVTTLESVTFGSVRQRLAKLLLD
                     TSDQAGSNTFPLPMTHQELASRLGTVREVVSRNLSRFQVEGLIGISERQIAIANRTGL
                     EREAATEL"
     gene            complement(17843..18952)
                     /locus_tag="VFQ79_22175"
     CDS             complement(17843..18952)
                     /locus_tag="VFQ79_22175"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0142445.1"
                     /translation="MRPVVLAALVSVSTLCPLFGALPIPGDAREGEKIFTEQRCITCH
                     SIGGVGGKAAPDLGKRTGRSYTPNMMAALMWNHAPVMWSAMEKQGVDKPRLSRENAAD
                     LFAYFYAKRYFDHPGDAGRGKRLFQAKGCDGCHGISEAKAGGGKPVSQWESLGDSIEF
                     AKALWNHSSEMSQAAAQKNLKWPELTSQQMTDLLVFFQNLPEAKKRQASFFPADATTG
                     QKLFELKGCADCHKESNSLENRFSFRSMQDFAAAMWNHAPLMKHHPELNTEEMRRIVG
                     YLWSIQFFEPKGDPQVGRRVFENKRCAMCHENAQSGAPRLAGQAPGTSFEMVSALWHH
                     GPAMLDKMNQQSLRWPRFNAREMANLVAYLNTLAK"
     gene            complement(18973..>19454)
                     /locus_tag="VFQ79_22180"
     CDS             complement(18973..>19454)
                     /locus_tag="VFQ79_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142446.1"
                     /translation="AYFWLVKNQNPAPAIGLDFTSAAGSIGAQWTPSSNKWVSVIGEY
                     TRSKLDSNISFIDPTDFSRTRSIYEDHAHTVTGMLDVNLPAIHGQQPKLTVGGSMFLS
                     TGSRPTDYYQPVGRFLVPFGKHVHWYAEWRWYGFFETLYRYEEFRTHHFITGFRLNL"
BASE COUNT         4003 a         5943 c         5569 g         3939 t
ORIGIN      
        1 agtaaatggg ctcggcgact accaggtgct ccagcacctc taaaatattc cacgactccg
       61 ccgacggccg gaattgaagt tgggcggaag agagatctgc tatcgcatcc gatagttcct
      121 gttctgtcat ctccaagtgc gcgacgagac gctgccgttc cagccgggtc agagattccg
      181 ccaggacgaa gtttgtactc cctgtggcga tgacaacaac gatcaatgcg aaggacaggg
      241 cgtgcatatc ggagacgcct ggtggctaga ccaagtgtac ccaagtggcg gccgccaaat
      301 ttcgttgcag attccgcttc cttccatagg aggaccatcc catggctaca caaactttaa
      361 cggaaacccc aaccaacacc ggcagcaacg gcaagaacgg ctgctgcaac ggaatccgaa
      421 tcgacaaatg cggcgacgtg catatccaca attgtgcgcc gccggaatac tgcaacaagt
      481 atccgcccga agcggctcaa ggcacttgca ttccgattgt tgccggagcc aaacacaaac
      541 tgagccgcca gttgaaaatg accgaactgg ccgatacagt acgagtcccc agttccattg
      601 caaccagcat cgttcacatg gctcgccggt tcgtcctcgg acatcaaccg gccaacgcac
      661 tcgagaccgc cgcctttccg gtgctcgagc ggatctccgg tgaaatgctt tcctgcactg
      721 tggatgcgtt tgacgcgctc acggccaggc agcgcaagcg cctttttgcc gattccctgc
      781 tgatggatcc ggaccagccg atcagcgaag agatgctggt ggccgcgctg gccgaggaaa
      841 tcaaggggcg ggccggtatg gagatctttg gagatccgct ggcgggggaa caggagcgtc
      901 ccggcaaaat ccgcgtgttt ccaccttccg gtgagatacc gccatcgcag gtgcgtatct
      961 gccgtgtgaa tgggcttcgc acagccaact tcgtcccgtt gccgccgata ggtgacttcc
     1021 tgccgagcga actccatcag gactgctcac ccatcatcgt caacggccaa ccccaggtgg
     1081 tctgccaggt acgcaccatg aactgccccg gcagcgtgat tgattcgacg gtctgcgcgc
     1141 gggtactgga agtagccagc ggagacagcc tgacgctgga aggggtgaat tacttcagta
     1201 cggacgcaac ggtccgaatc accgacagag aaacgggagc gacagttcgc gatgtggagg
     1261 cgcacgtttg gggcgacgtg gacacgccgg tgacagaagt tgtaaatggc gagaccgtcc
     1321 tgatcaacga ctgccgtgta catgacaggc tgacgtttcg catacctgag gacctgtcgc
     1381 ccggcatcta tcagatccag gtgttcgtgc cgaatatcac aggtattccg gcgttcggcg
     1441 actttctcaa ctccgacggc gaattcatcc aggtagtaac gccgtctacg gcgcgttttc
     1501 agatggtgac cgagaagatc gttgcccgcc aggagacatc gccggcgcgg ttcggctcgg
     1561 atgaagttgg tctccaggcc atggctttcg ctcttttcgc cgacgggacc ttcggcgatg
     1621 cgcaggagga gaagttcaaa gatatccggg atgtcgattt cgattccggg acgtcgagag
     1681 atatcaccag actcgtgttc agtcatgacc agccgatcct tggtatggct ctctcattcg
     1741 ccggcttcga gattgacagc gaacgcgctt tcaacgagtt gattacttcg cgaatggact
     1801 tttttaccca gctcatcaag gatcaggcga agttcattgg ggccgccatt accgccctgg
     1861 gaggcgcgtc cgcgctgacg aagctgggaa cagtcgggct gtggatcgcc ggcatcgcgg
     1921 cggcggttgt gcttgccatc aacgtgattg ccgctctgtg ggcgcctgcg gatccgatca
     1981 ttcgcgattc gttcggcttc tcaaccactg atctggaccg gctgaccagt gccagcttcc
     2041 cggcgccgcc tccctcaacg ttcgaaacgg agggagatgg cgagatatcg gtcaacgtta
     2101 ataagacagt cccacccgag aaagtaccct tgcagtaccg agagacccgc gagtacgtca
     2161 gcggcacgga agacagccgg tacgagatca cgtaccggca caaccgtatc gcatgaacca
     2221 attcttcgcg gaatgaccgg gcctggggag gcgataatca gcagccgccc caggcccggt
     2281 gcctttagtc acctccgtgc atcacgatag aagaagtagt cggtggtata tggcaccagc
     2341 acgactgcgc cgtaagccgg ctcgttgcag ccgctggttg gcgcgacgcc acccgaagtg
     2401 ttcagcctct gaatgtaagt agtctgcaca agcgccgacc caccgatggg cccacgctgt
     2461 gcgccgacgg cttcaagaag cagccatggg atagcgcccg cggcgacaaa gttcggatcc
     2521 gtcgagcttg ccttaaccct gccccacacc gcgctcgtat caagcgagtg ctgccatacc
     2581 ggtcgggccg taccggcctc cgccggattg gagctaagaa agtgtgtggc aatctgctgg
     2641 cggatttcgc cttgtatcca gggaatcgtg agaaacaagg ttgcctgcgg ccccaggaac
     2701 ctccaggccg ggcccccggc tcccggtagg cagacgtagt tctgtgtgcc aaccgcatag
     2761 cccttaaggt agagcgtgtt ccctgcgggc acttgcagct ccgaaggcat agaaggcact
     2821 gtcatcttcg cagcctgatc catcgccgcc gatggcaggc tgaaaacgat cgcattggct
     2881 gcgatcaagc gtagtaaatg gtgggacata aactatcctt tctgttggtg aagcgtgatc
     2941 ggagcgctaa atttgcgatt cgaagaacgc ttcagggaca ccgcgtgtgc gcattgccgc
     3001 gcgctatgat acagtcgacc ttttggaaag gaccggcttc gtcccgtgag cgcaagtgtg
     3061 cccgagctca ttgcaaggct gcgtaccgga gacgaatccg ccctgggcga actcactccg
     3121 acgttatacg gcgagctgcg ccggatcgct tcccggcacc tgcgcggcga gcgttcgaat
     3181 catacgctac aggcgacagc gctggtacac gaggcctacc tgaaactgat tggtgccagg
     3241 gaacggcagt tcgccgacga ggtacatttc ctggccgtcg cgtcacgagt catgcggcaa
     3301 gtcctggtgg actatgcaag atcgcggtcc acacagaagc gggccggcgg cgcgagggtc
     3361 gagccgctat ggacgacgac tctcgaggtg aagagcgacg acggtatgga actgatcgag
     3421 ttgattgaac tggatcaggc cattgaagcc ctcgccgcgg agaatgagcc cctggctcgt
     3481 ttgatcgaaa tgcgctactt cggcggcatg accgcggagg aatcggccga agcgctcggc
     3541 ctgtccgttc atgtggtccg tcacgatctg cgatttgcac aagcctggct gcgaagaaaa
     3601 ctgagcccgt agttacggcg cccaagcgcc ctgccaaagt tttttcgggt tgcctcattt
     3661 tttttgatcc tttgtgaagc tgtttttcgc atataaagga tgtgattggc gaagaagtcc
     3721 ggcactggtt cgaacaggcc gtgaacgtaa cggccggcgg ccgcaccgct tttctcaaat
     3781 ccaactgccg tgacgaagcc gcacgctctg aagtcctgtc gcttctcgaa tacgatgccg
     3841 gcaacaccgg gccggcgccg ggcctgccgg ttcccgaggc actgttccaa gaggccattg
     3901 gaaccatgct ggggaaaggg aatccggtgt tacccgcacg gcgagccggc cccttcgaac
     3961 tggggcggct cctcggatcg ggcggcatgg gtttcgttta tgaggggcac cgcgtcgacg
     4021 gtgcagttcg ccagcgcgtg gcggtcaaat tcgcccaggt cttgcccgga accagccggg
     4081 aacttcgcga gagcgctcat cggcgcttct accgtgagcg ccagatgctc gcgtcgctgc
     4141 ggcatcctta catcgcgggc ctgatcgacg ccagcaccac tgccgacggc attccgtacg
     4201 ccgtgatcga acaggtcgat ggtgtgccga tcgacagcta ttgcgatacc tggcatccag
     4261 accaggccga acgcatccgg ctgattctga agctctgcga tgcggtccag ttcgctcatc
     4321 gcaacctgat cgtacacagt gatatcaagc cggataacgt gctggtcacg gccgacggaa
     4381 ttccaaagct gattgacttc ggtgtcgcca gcgacctggc agaagaagcg acgctcacgg
     4441 caatgcgggc attcacaccc ggctatgcca gccccgaaca atcacgcggg ctccccgcta
     4501 cggtcgccac ggatgtttac ggaattgggg cggtgttata ccgcgtactg acgggcgcaa
     4561 agccgcgcga attgaaaagc gggccgctta gcgaagtggt ccggtgcatt tcggaggaag
     4621 acgtcatccg gccgtcgtcg atcaaaccgg agctgaaggg cgatctcgag aacatcctcc
     4681 tgaaagcgct tcagcgcgat ccccaacggc gttacggatc ggtcccggaa ctggcggacg
     4741 acctgaaccg tttcctcgca cggcgtcccg tgcgagcctc accagactcg atgctgtacc
     4801 gggccagccg ttttgtgcgc cgccactggg tgtcgctggc cgccgccgct acgcttatcc
     4861 tctccctggc gattgtcacg atcatatctg tacggcaaag ggagcacgca atgcagcgtg
     4921 cgatggaaac ccgccggctt gccgaccggc ttctgtttga agttcacgat gaaatcgcag
     4981 gcgtgctggg aggcaccaag gctcgcgaga agctgggcgc catcgcggtg cagtacctgg
     5041 aaggtctcga acgggattat ggccgtgatc cggagctggc ctgggaactt ctcaacgcct
     5101 attcccggtt ggggcagagc cgtggcgggg ccgcatcgag tgtcggcgat accaggagcg
     5161 gtacccattt cgcgatgaag actttggagt tgggcgcggt cgtggaagcc gctgctcccg
     5221 gaaccgatcg tttggacaag ctgttcgccg tctatgaaag cctggtaccc atttttcagg
     5281 aagcgggact gcccggacag cagcgcgaag ctattgatcg aatggtgcac ctggcgcccc
     5341 acctccaacc ccttcggcag gcgcaggcgt taaaggaact ggggcgctat ctggatgcaa
     5401 acggttccgt agcacaaggc gcggaagctt tcgagcgctc gcttacgatt ttacggacgc
     5461 tctcccggag cccatcgaag cccgccggga cggaagcaca actgatcagc acactggtcg
     5521 gctttggccg ggcacaagcg ttaaggggcg atttttccgg cgccgtggcc agtttgcagg
     5581 aagccatccg gctatccggg aacagcaccg cctcagatcc gcacagggcc aagagcgccc
     5641 gccagttgta ttggagccac attgcgctcg gagatgtttt cggatctcct ttgcgcttta
     5701 acatgggacg ctctgatgat gccgtggagc actatgagaa ggcgcgcagc atcgcggaaa
     5761 atctggtaaa ggccgacccg gggaatgaag ccgcaaaact ggacctcgcc agggcattca
     5821 gcagacaggg agtggcactc gcagcttccc agcctgcaca atcactggta ctattggagc
     5881 gttcccattc gctggcactg gaaacgtctt atcagaatca ttcgggactg gattcgcgtt
     5941 tcgcttatct gaccagttca gttgaaccgc tggtgcggct tggctatttc gagcgggcgc
     6001 gtttgcacat cgcggaagcc cgccgcttgc tgaaagaact gcggcaggca ggcgtcaggg
     6061 tgaatgaaaa caatcttctc agagcggatg caatccgtct ctatgcaagc ggccatgcga
     6121 aggaagccct ggcggaagcc caaaaacacc tcgccctggt tccggagaca acgagctccg
     6181 ttctgagcga taacttcgag cgggtggaac tgctcgagcg gatacgcatt tacgcagcag
     6241 gcatcgatgc caccgcttac gcttccgcta ccgcgcagct cgcacggatc tgggagaact
     6301 tgaagacaac tgtttcacga cgtaatccag ccggttgaga aatgcgcgaa cgtgatggtt
     6361 tcatacgggc gcgattcccc gcactcatag agacagccca cggcgccgtc gcgaagtacc
     6421 gccaacgatg aataggcagc cgggccttcg tggagcaacc tggcgtgagg ccacgtgcgg
     6481 ccgccgtcgc tgctggcccg caccgtcatc cgcacacgct tctccgcagc gggattggag
     6541 aacaacagcc acttctcgtg acgaatcaga ctggcctggc acccgggctc aatcaacgtt
     6601 tcgtccggtc cgaactcgcc ccaggtcatt cctccgtcct tgctgcgccc taccagccgc
     6661 cggttgccgc gatcggaacg gcagttgagc accaggctcc cgtccggcag ctcggcggcc
     6721 tgggactcgc cggtgccttc gggcaattcg ccgccgcgct tccacgttcg gccatgatcg
     6781 tcgctataga aaacgtaaga gtgctcgcgc cacgttcccg tgggcgcata atcgcacggg
     6841 accacgatac gtccgctctt gagttggatc gcgttgcccg gacccgtggc acaccatgtc
     6901 caggacggat cgcgcgcgga gccgctaatc tccaccggtt tctcccaagt ggcgccgtcg
     6961 tctgcgctgc ggcttaacca catccgccgg atcgccgctc cttcgagaat ctgcttctgc
     7021 gaaatgtgcc cgggattgcc ggtcagcggc agccagatcg ttcccgtctt gcgatcctgc
     7081 accggacagg gatttccgat ggtgtcgggc ccatggtccg ccaccaactg taagggagac
     7141 caagtgatcc ccccatcctt gctgcgcttg agcaccaggt cgatatttcc ggtgtcgctg
     7201 gaactgtccc gccttccctc gcagaaagcc agcagcgtgc cgttcctggt agtgagcagg
     7261 gctggaatgc gaaacgtgtg ataaccgtcg tcaccggcgc gaaagactac cgtgttccga
     7321 ggcacgcctc cagcggctgc gaggccgaga agaagagatc gccgtgtcaa catgacagtt
     7381 ccttaaaact atagaggtga gtccgtttat ttgccttttg tggcgagttt cagcattcca
     7441 ctcctgaaag cctgaaaact cggcgactgg ttgcgttcca cgtcgatatg cggtccaaca
     7501 agatcggccc atcgacactt ttcttctcct gctcgcggat agccgtccga ttttaacttt
     7561 gcggacccgc ccggaaagac ggcgtcggct aagacctccc atgtcccaca gattgaatcc
     7621 ggcacgtagg aattcaaaac agttgctttt gctcgcggaa acaccttgag gatcgcgcaa
     7681 cgatcaccaa gtagccaagc ttccgcctcc tcgatcacca agcgaaataa aaccctcggc
     7741 cgtggatgac agttctgcag agtctggagc aactcttcct tgaagccgat gcactcccgg
     7801 ttatcgaggt ccactaccac cacgaccgcg gcatcctggt tttgaagact cttaccatag
     7861 ccaccaagta tcctcggtaa ttgatttaaa agcacccgct tccagggatc tgtcttcccg
     7921 cgcaggtccg gaggaactcg accaatcccc ctgtaagcgt gagtccgcca cgtatgtaaa
     7981 tcccctttcg cacccaaaat cttcggcagc agtgactcga gaagcaactc accagatctg
     8041 tcctcgatca ggatctcaaa gtgcatcagg ccgacctcgc gtccagatag tcgctgtacc
     8101 acaggctacc cagtggaagg ccgtgttcca ccatgctctg gactgtcgta tcggaactgg
     8161 cccttcttat gacagaaaag ccatctttcc ccttttcaag cacccaagtc tcctcgggtc
     8221 ttaacgcgtc gacgaaatac ggttgatgtg tcgtcacgaa gatctgaggg gcattcttgc
     8281 gaccggttgc gtgtgatcgg aattctgacg ccaacgtctc caatagcttg tggtagagtc
     8341 cattctccgg ttcctcaata cagataaaag gtgctggatc cggatcttca agcagcagca
     8401 tatatgcaaa catcttcaga gtgccgtcgg acatctgctg ggcaaagaac ggatcttgaa
     8461 acccacgatc gttgaaccga agcagcaagc gcccgtcgtc cgtcttcttc gtatcgatct
     8521 tctcgatacc agggatcttc gaagcgattc ggtttagtat ggactggaat cgttgtttgt
     8581 gttcccgttc catgaactga acgacattgc cgagattgtc cccgtgcatg ttcaggtgct
     8641 tctgcggccc cgccataggc aagctgcgcg cggcgtccgg cgtaaaataa cttagatacc
     8701 agctctcaag aaaccgtcga aacttggcga tcctcgggtg agatctcagc gttcccagtg
     8761 tggcgatgcc tagccttctg ctatctgtta gctcgacagg aatccgtcct gcttcttcgc
     8821 cttcgaaact ctcttcgcca gcccacgcct cgccctgccc tgctctcaaa tagagaaacg
     8881 aaagcggccg tccgcggctc tgacctttgc gtctttgccg gagtcgctcg tcggcaacga
     8941 acggccggcc cgatccgtca cgactgattg acaactcgta tgtgatcggc cggtcaccgg
     9001 ggctttcgcg atagtagaca tcgaaacgaa ttggctcctt gaccccggac gaaaccaagc
     9061 gatcgaaccc acctctctgc ttcaaatcac acgcctcctc cacacctacc gccaagcagt
     9121 ctgaaaggaa tccgaaagcg tcaaataagg tgctcttgcc gactccgttt tttccaataa
     9181 ctgctacgag agcagtaagc ggtggcgttc tttgctggtt ccaaagcctg ccgatggtta
     9241 tgtctttgag ggcgcggtag ttccgtacgt gaaaaccttc aatcaaggcc atagccttcc
     9301 tccttcgtag gccggcatac gttctccgga gcacgtgtac ctgcggctct ctctatgtgc
     9361 tgtcatcgcc agcttagccg gaaacgtcat tatagcggcc tgtcctctgt tgcaaaacgg
     9421 acccgcaacc tatgtgatcg gagccagcac agacggagaa gccgtttcac gccgctacca
     9481 tcggcgtgcc gcgtttccac tcgtcaagcg gcactcgccg tcgctcgtat ggtgatacgc
     9541 tgtgaaccaa atgctcaacc gccgggtctt tctgatcact cccgccgctc tgttgcacgg
     9601 tgccgctctc gatcgccgcg ccgtcatcca gcagcgcgcg gacgccctcg ccacgtttcc
     9661 cggccagcgg gtcgaagcaa gccgctctga aaaggccgat caaatcgccg ccggaacggt
     9721 cttcttttac agccgaacgc ctgtgaaggt tggcctgcgt aatatcgatt ggagcggcgg
     9781 ccacatcaaa catcaggagt ggcccgcaca actcaaccgg ttcttccatc ttggtccgct
     9841 cgcctccgcc tatctcgcta cgggcgatga agtctatgcc cgggccgccc gcgcctacat
     9901 cgaggactgg ctgcgcgaag acccctatcc cgaaacacga agctttcgcc ccggcgacaa
     9961 cgccctgacg atgagcattc gcctcggcac cagcgtcgcc tccggttgga gcggcacgct
    10021 tcccgtcttt cttcggagcc ctgccttcga cgaccaattt ctcgatcgca tcttccactc
    10081 actcgctcgc caggcccaat ttcttgccgc gcgtctaacg gctgtgggca actggcgtat
    10141 ctccgaactc gacgccctgg tcttcatcgc actgcgcttt cccttcctcg acggtgcgcc
    10201 tgtgcttctt gaaaccggca tcaccgggat gcgcaatgcc ctggcaaccc agttcctacc
    10261 cgacggcgtc cacatcgagc gtacgcccgg ctatcacgac tggatgacgc aggtcgccgt
    10321 caattacgcc gacctcgccc ggcgcttccc ggacgccgac gcccaagtgg atgctgcccg
    10381 cgccgtgcgc gctctcgact acggcgcgca aagcgaattg ttcggcgtta atgactccat
    10441 cgctccaaac ctggatccgc cctccttttc caggctggaa cgaagaagga attccctcgc
    10501 cgtcctgcac ctcgcggatc gcacaccccc ggaaccgccg ctcgaccagg tgttcccgga
    10561 tgcgggtcag gtcttcagcc gaaccgcatg gaagagcggt gccgattacc tcgcgttcga
    10621 tgcgagtacc tggggcggcg gtcacagtca cctctcgcgc ctctccttcg cttttcgttc
    10681 cggcggtcgc gcactggttg ccgatcccgg catcctctcc tatgagatgt ccgatcctcg
    10741 cgctccctac ggcaaatcca cggccgctca cacgacgctc aacgtcgatg gctacaatca
    10801 gtccggcgcc tccggccagc ttctccgcac tgcgtttacc ccggacatcg cactgattca
    10861 cgcacgctac gaaggcggct ggtggaaggg gcagtaccgg tggaacttcg gccagggccg
    10921 cggtgaaggt atctggggcg agcaccagcg cattctgttc tgggtgaaga gggagtacct
    10981 cgtggttatc gattcactca ccaccgatgc cggccacgag attcgcaatt gctggcaaat
    11041 gggaccagtc gagaagtggg cgcaggatgc ggaggggctg cgctggtggt cggccaatcg
    11101 tgacgtcaac gttttgctcc aactggcggt cgcgcccaag gagacaacaa tgcagtgctt
    11161 cgaaggaagc aacaagccgc tgcgtggttg ggtggggcag cacggtgaca actccgtggc
    11221 cgcgccgctg gttgagttcc gctatccctc ggtcctcaac cggcctgtcg tcaccgcggc
    11281 gctgctggcg ccgtcacgtg gtgcggtgcc tccttcctgg cgcatcacgg cggcgggcgc
    11341 tacctccaac gtagggcacc tggaaatcgt aacacccaat ggcgcaaccg acctgctggc
    11401 ctggacgcct ggcctggcgc ttccaataga ggactcacag ccgttcgtca cggatgcacg
    11461 cctggtctgg acacggcagg gaaccggccg ccgcttcctg ttggacggca catacctcaa
    11521 gtaagcacca ggtccccgcg cttgctacct tgcaggctcc gtactgaact tgtacaccgt
    11581 ggtgctctga tacttcgcgc cgggtctgag cacaaccgaa ggaaactgtg gctgattcgg
    11641 tgagtcggga aagtgctgcg tctccaggca aaagccatag cgatggccgt aagcggtgcc
    11701 gccttttccc cggatcgtcc catccagaaa attgccggta taaaactgca caccgggctc
    11761 cgtcgtcagc acctccatca cgcggccgga accaggctcc gtcacgcgcg cggccgggcg
    11821 cagggacccg gccgcgccat tcaaaacaaa attgtggtcg tagccctttc cgtaaactaa
    11881 ttgctgatcc ttgccgtcta tccgctcgcc gatggcatgc ggttgtctga agtcgaacgg
    11941 cgtgtccgct accgcccgca actcgccggt gggaattaaa ccttgattta ccggagtgaa
    12001 cctgtctgcg ttgatgctga tgcggtgtgc cagtatttct ccgtttcccg cgcctgccag
    12061 attgaaataa gagtgattcg tcagattcac cacggtcggc ttgccggcag tggcttcgta
    12121 atcgaggcgc agttcgttgt cgccggtgag cgtgtaacga accgttgccg tgagatttcc
    12181 cgggtaaccc tcttccccgt cttcgctgag ataggtgaac tccaggccgt catcaccgtc
    12241 tgcccgcggc tccttcagct tccataccgc tttatcgaac ccccggatcc ctccatgaag
    12301 gtggttctcg ccattgttgc gcgccaattt atattccacg tcgtccagca cgaaccgccc
    12361 gccggctatc cggtttccgt aacggccgat caccgcaccg aaataaggat gttcgcccag
    12421 atatccgtcg agcgaatcga acccaagaac gacatccgtg gcgttgccgt tccggtcggg
    12481 aaccttcagt gagaccagga ttgctccata atcggtaatg ctcgcttcca cgcctttaga
    12541 attcctcagc gtatagagtt gcacgggctc tcctttggcc gttttgccga actggcgtac
    12601 ggccgcggtg ccggccgcgc gttcgccggg gcgattgcag cccgtcacgg caagtgaaat
    12661 ggaaagaagg attacagtcg gggtgagttg catgtggagg gcttccgcaa gacgtcataa
    12721 tatcattggg cagtcatccc gccgcagagg gcgaaccaaa aacagataat atgagtagca
    12781 aaccacaagc agtatgagtt ccgggtgtca ttatgggcga gtcaaagtct aagccgtcct
    12841 ccgacagttc ccgcccggcc gtacggcgct atttggccgc cctgttgctg gtcacggctg
    12901 cctctctgct gcgcctcgtc ctcgatccca ttctggcgga gaggagcccg tatcttattt
    12961 ttctttccgc cgcagcggcg gccgcgtggt acggagggtt cgggcccggg ctgcttgcaa
    13021 cggtccttgg cgtatttggc gcgctggcct tcgccgttga cccgttatta actttccgcg
    13081 tggacactcc gggcgactgg ctggctctgg ccctatacct gatcttcggc gtttttgtaa
    13141 gcgcattgac ggagaaactc catcgcacga aacgccgcgc actggatgcg gaagcctccg
    13201 agcgccgtca acgattgttg gcgcagcaga cgctggccgg tatcggcgac gccgtgttgt
    13261 ccactgacgc tttgggccgc atcgaattca tgaatgcgat cgccgagcag ctcaccggct
    13321 ggactatgga tgatgcacgc gggagacctg tccggcaagt cttccagatc gccaacgagt
    13381 acaccggagc gcccgccgac gatccgatta cccgtgttct tcgggagggc atcatcgccg
    13441 gtctcgcgaa tcacacggtc ctcatcaaaa gagatggcag caggatcccc atcgacgaca
    13501 gcggtgcgcc catccgtgat gaatcgggga atctgattgg atccgttctg gtgtttcgca
    13561 atattgccga gcgccgccgc acggagcagg aacgcgagcg gctcattcga gatctgaccg
    13621 aaacccggga tatcatcaag tccgtatttc gaaaagcgcc cgtcggcctc gctctttggg
    13681 acctggatct gcgctttacc cgggtaaatg aggcgctggt ggaaatgacc gggttgccga
    13741 tgggggcgca tatcggtaag acgatcgacg aactgctgcc cggcctccct ccggaggtaa
    13801 ccgaggcgat gcggcaggtc cgcgagacca gaactccgtt ccgccacgag acggacggcg
    13861 aaactccggc ctcgccaggt aaacggagat cctgggccat ccactattac cccattcgcg
    13921 tggacgagaa cattgcgggc atcggtgcgg tattccagga aattaccgag aagaaggcct
    13981 gggaggccga gctggagctg gcgaacgaca accttaagca gttcgcctac gccgcctcgc
    14041 atgacttgca ggagccgctg cgtatgatca ccagttatac gcaactgctg aagaagcgct
    14101 attacgacaa actcgacagc gacgggcggg aattcctgga ctttgccatg gacggcgcga
    14161 accgcatgcg gacgctgctc gaggcgctac gggactactg gagggcgggt gaagacggtg
    14221 gagaaccggt cacagtggat tgtaacgccg cgctggaaac cgctttgggc aatcttcgtg
    14281 cttcgctggc ggagaccggc gctgttgtca cccacgacga ccttccgaaa gtagtcgctc
    14341 atgaagtgcc actggttcaa atcttccaga atctgatcgg caacgcgctc aagtaccaaa
    14401 acgaagcgca acctcgagtg catatctcag cctccggcca tggcgctcag tgtgttttct
    14461 cggtgcggga taacggaata ggaatcgaca agagacaatt accgaagctt tttggcgcgt
    14521 tccggcggtt gcacggaagc aagtatcctg gcactggaat gggactcgcg ctttgccaca
    14581 agatcgtcga gcgctacggc ggtaaaattt gggtggactc ggaaagcagc aaaggctcca
    14641 cgttctattt cacactgccg ctggcgagcg ccgagacggc agccggccgc gcatcccggt
    14701 tgggcgagcg gccggctacg tgaaaccaac tctatctttg cttcacactc cgacgagttc
    14761 tccctcttcc tgttccacgg gcaattcggc cggtattggc ctgagcgccc gcagatggaa
    14821 gaggccgtaa acggctgccg ccgcgatcgg gagcacaccg gcgacgatga atacagtgtc
    14881 cggcaccatt cgcagccaca gcagtttatt caccagaggc tgctgataaa actcgaacga
    14941 acgggccgac cagaagccgt tccggaacga ctcgagcgcc tgcatcactc cgaccgggaa
    15001 cagtgtcgtc acgatcatgc ccatgaggcc gatattgagg ccccagaagc ctgtcatgat
    15061 ccacttctct ttccagtatt tcggatcgac gatgttccgc agcgagaacg ccgccagcgc
    15121 gatcgccagc atgccgtaca cgcccattaa cgctccgtgg ccgtgtgccg cggtgaggta
    15181 cgagccgtgc tggaagtagc tgacgaacgg tagattgatc aggaagccga gtgcgccggc
    15241 tccgaagagg ttccagaatg ccgttgccac caggaaccag aatgccgctt tatacgggaa
    15301 ttcgattcca ccgtcgcgga tcaccttgta ttggccataa gcttccacca tcaacagcgt
    15361 gagtggaatc acttcgagcg ccgaaaagat cgcgccgagc gccatccaac tttctggcgc
    15421 accgatccag taatagtgat gtccggtgcc gatgatgccg ctgccgagca ggatgatgag
    15481 ctggaagtac agtgcgcgaa gagtcgattg cgccgtcacc aggcccaggc gaaccatgag
    15541 gaagccgatg accacgacag cgaaaacttc gaacatgccc tccacccaca ggtggatgag
    15601 ccaccagcgc cagtagtccg ccatggtgat atggctgttg cggtcgataa acagagcgaa
    15661 acagtaaaaa atcggaatag ctgccgacga gtacaacagc aggtgagtga gcccgccgcg
    15721 gtcaccctcg ctttgtagcg ccgccttgag gccgcggtac acgatgtaca accagatgag
    15781 catgccggta atcagcagat acatccagat gcgcccgagt tccagatact cccacccctg
    15841 cgtgccgaac caccaccagg tttcaccaag gtagcctttc accgatagat actcgcccgt
    15901 catgctcccg acgacaacta cgaccagcgc ccagaacaga atgttgacca gatgtttctg
    15961 gcctttcggt tcccggccgc tcacaagagg cgcgatgaag atgcccattc ccaaccaggc
    16021 ggtcgcaatc cagaagatcg ccagttgcag gtgccagccg cgagcaatat taaacggaag
    16081 atactgcgaa agatcaaggc cgtaaaaggt agccccctcc gcgtagtcgt gcgccatctt
    16141 gccgccgagc agagtttgca cgaggaagag cagaatggcg accacgaaga acttggccgc
    16201 cgcgcgctgc gaaggcgtca gcggactgcg ggcgacatcg aaccgcagcc gcggattttc
    16261 cacatccacc gggctcagac gataacgctg atgaagatac aggatcagcg ccaaaaacat
    16321 cagcaggccg gctacgctcg ccgccgacca catcatggaa ccggcggttg ccgtattacc
    16381 ggcagttttg tcgtagggcc agttgttcgt gtaggaatga tccgcctccg gacgcttggc
    16441 taccgacagc caggctgtcc aggtaacgaa cgccgccagt tccttggccg cttgccggtc
    16501 gagcgtaata tttccaccac cctcgcgctc gggcaacagc gcgcccttcg gcagcgaacg
    16561 ggtcgtatcg ccttttgcga acgtcctttc atagtggtcg acgatccggt tccaacccgc
    16621 tacctgtctt tctgtaaaac gcaagatacc attttcataa gtattctgcc gcaattccga
    16681 agcgatttcc gcgtcaattg cagctttttg tccaaggtcc aaggtttcgt aagcgccgcc
    16741 gctgccggcc gcctgcatgc cggcggtcat gatgtgcagc gcttccgcgg tgtagtccgg
    16801 cccaagatat gcgccgtgcc ctaacaccga cccgtagttc atgagatttc gttttcgaaa
    16861 gagatcttga ccattttgaa tatcttccac tgtgaacagc gtttgccctc ttgggccaac
    16921 tgtcacttcg ggaatcggtg cgcggcttcg atacatcgaa tacccgccga cgatcatgac
    16981 cgtgaaaccg aacagaagcg tcagaagaag tccgtgcttc caaagtcctg atttttcctg
    17041 aaccataata aatgtgagtc ctggaacaaa tgtaattcca gggaggtatt cttacctatg
    17101 accgaagtca cagagggaat atatattaga tttatagttc ggtggcggct tcgcgctcga
    17161 ggccggtacg attcgcgatc gcgatctgcc gttcggaaat gccgatcaaa ccttctactt
    17221 gaaaccggct gagattgcgc gagacgactt cccgcacggt gccaagacgc gaggcgagtt
    17281 cctgatgcgt catgggaagg gggaacgtgt tcgaaccagc ctggtcgctg gtatcgagca
    17341 acaacttcgc aagccgctgc cgcacacttc cgaacgtgac gctttcgagc gtcgtcacca
    17401 ggtgccgcaa tcgctttccg accacggcca gcaccttcag cgcaacatcc ggatactggc
    17461 ggcacacctg tgagaactca cgttttggaa tcagaatggc acggacatcg ttgatggcgg
    17521 cgacggacgc ggggtaggcg ccgccatcga acatcggcaa ttccgctacg gtgcccggcg
    17581 cctgctcgag cgcgatcgtc atctcgcggc ccgccatcga cgtcttgtag atcttcaccg
    17641 cgccctgcgc gatgacatac agaccgtcgc actcttcgcc ctcgttgaaa agcacgtcgc
    17701 cgcttcgata gcgcttctcg acagcgtgtg acgcgatcgc ttccagctca ggggtgctca
    17761 agccggcgaa gagttgaatc cgccggaaga tctcggcttt attctcgttc aaaccaacga
    17821 ttctagcagg aacagcggca tctcactttg ccagcgtgtt gagatatgcc accaggttcg
    17881 ccatctccct cgcgttgaag cggggccacc gtaagctctg ctgattcatc ttatcgagca
    17941 tcgccggtcc gtggtgccac agagccgaca ccatctcgaa gctcgttccg ggcgcctgcc
    18001 cggcgagacg gggcgctccg ctctgcgcgt tttcatggca catcgcgcag cgcttgttct
    18061 cgaagacgcg gcggcccacc tgcggatcgc cctttggctc gaagaactga atggaccaca
    18121 ggtagcctac aatccggcgc atctcttccg tgttgagctc gggatgatgc ttcatcagag
    18181 gggcatggtt ccacatggcg gcggcgaaat cctgcatgga ccggaaggaa aaccggttct
    18241 cgagcgagtt cgattcctta tggcaatccg cgcagccctt cagctcgaag agtttctgac
    18301 ccgtggtggc atcggctgga aagaacgatg cctgccgctt ttttgcctcc ggcagattct
    18361 ggaagaacac cagaagatcc gtcatctgct gcgacgtcaa ctccggccac ttcagattct
    18421 tctgcgccgc ggcctggctc atctccgaag agtgattcca aagcgccttg gcaaattcga
    18481 tgctgtcgcc cagcgattcc cattgggaaa cgggtttccc tcccccggct ttcgcttcgg
    18541 aaatgccgtg gcagccatcg caacccttgg cttgaaacag ccgcttaccg cgtccggcgt
    18601 ctcccgggtg gtcgaagtat cgcttcgcat aaaaataggc gaacagatcg gcggcatttt
    18661 cccggctcag tcggggcttg tccacaccct gcttttccat ggcgctccac attaccggtg
    18721 cgtgattcca catgagtgcc gccatcatgt tgggagtata ggagcgcccg gtgcgtttgc
    18781 ctaaatcggg cgcggccttg cctcccaccc cgccaatgct gtggcaggtt atgcagcgtt
    18841 gttccgtgaa gatcttctcc ccttcgcggg cgtcgccggg tatcggcagc gcgccgaaca
    18901 gaggacataa ggtggataca cttacgagag cagcgagtac gacggggcgc ataagccctc
    18961 catattacag ctctacaagt tcagtctgaa accggtgatg aaatgatgag tccggaattc
    19021 ttcgtacctg tagagcgtct cgaagaaacc gtaccagcgc cattcggcgt accagtgcac
    19081 gtgcttgccg aatggaacca gaaaacgccc aaccggctgg tagtaatcgg tggggcggct
    19141 gccggtggag aggaacatcg aaccgcccac cgtcagcttt ggctgctggc cgtgaatggc
    19201 cggcagattc acatccagca tcccggtcac ggtgtgagcg tgatcctcat aaatcgaccg
    19261 ggtgcgagag aaatccgtcg gatcgataaa cgagatattg gaatcgagct tcgatcgcgt
    19321 gtactcccca ataacggata cccatttatt ggacgaaggg gtccattgcg caccgatact
    19381 gccggcggcg ctggtgaagt cgagaccgat ggccggcgcc ggattttgat ttttcaccag
    19441 ccagaaataa gcaa
//
LOCUS       DASTMK010000535         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_123650, whole genome shotgun sequence.
ACCESSION   DASTMK010000535 DASTMK010000000
VERSION     DASTMK010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123650"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..799
                     /gene="gpmA"
                     /locus_tag="VFQ79_22185"
     CDS             53..799
                     /gene="gpmA"
                     /locus_tag="VFQ79_22185"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEU0142447.1"
                     /translation="MKKLVLLRHGESVWNKENRFTGWTDVDLTEKGRQEALEGARAMK
                     EAGLEFDVAYTSVLKRAIRTLWIALDEMDLMWIPVHRSWRLNERHYGALQGLDKAETA
                     AKFGEDQVKIWRRSYDIPPPSLEKSDPRYPGNDPRYSGIPEQELPLAECLKDTVNRFI
                     PYWHEVIAPDVMSGRRVIIAAHGNSLRALVKYLDNISDQDILELNIPTGMPLVYELGD
                     NLKPIRSYYLGDPEKVRKAMEAVANQGKKK"
     gene            complement(863..1747)
                     /locus_tag="VFQ79_22190"
     CDS             complement(863..1747)
                     /locus_tag="VFQ79_22190"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142448.1"
                     /translation="MPLSAAGDYQAAKAQLETAWRLLKDIKPQDLRHVTVLNNLGSTR
                     ERLGAYNDAERCYREALKIRAALPEKERENSAWLLNNYAGLLVKKGNHKRAEALYHES
                     LEALIRTVGPIHADVAGIYNNLSELEFLHGNKKEAAKLLEQAKSVTEQAIGPNHVSVA
                     VILNNQSVLYEAEEEFDLAEAALQRALEIWRQASGPDHPATIRIVNNLALLRFHQRRF
                     EESDELLAGAIRTAEQKLGQNHPDLGLLWGNRAVVLRKLKQKSQAQAAETRATDILGR
                     DVSRTFRRHTVDVRDLGR"
     gene            complement(1960..>3154)
                     /locus_tag="VFQ79_22195"
     CDS             complement(1960..>3154)
                     /locus_tag="VFQ79_22195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142449.1"
                     /translation="TGIDINRLQYQQNVHRTGIEYLRPDGIPLRRTVFGGNGAFVRSA
                     WEAAAYVQDTWRVRPGIVLELGLRSDWDSILHNYNFSPRAAFAWSPKGWENTKISGGY
                     AIVYEATNLRLFTRPLDQYSLATTFNPDGSILRGPAISLYTIDSYNLRSPRYGQWSLG
                     VAQQLPSGFSLRVDLLRKRGREGLTYMDLTGGGKPLSNAVPYLIPAPEAYDVDSLYTL
                     SNMRKDVFDSVEFSIRQVLAGQYEWMASYVRSRALSNAVMDVNIDDPILVSDNAGPMP
                     WDTPHRFLSWGYLPLPFKNWAAAYLLEWRTGFPFSVVSADGAYTGGLNTQRFPQFFEL
                     NLHVERRFVLMGHKIAIRGGLNNVTNHRNYDTVNSNIASADFMRYYGGQRRALNFRIR
                     WLGRL"
BASE COUNT          690 a          880 c          922 g          662 t
ORIGIN      
        1 aaattcgccg gcaggaatgc cttccgccaa aaggtgggtt gaggagagac gcatgaagaa
       61 actggtgctg ctccgccacg gagaaagcgt ctggaacaaa gagaaccgct tcaccggctg
      121 gaccgatgtc gacctgacgg aaaagggcag gcaggaagct ctcgaaggcg cgcgcgccat
      181 gaaggaagcg ggtctcgagt ttgatgtagc gtacacttcc gttctcaagc gtgccatccg
      241 aacattgtgg atcgcgctcg atgagatgga tttgatgtgg attcccgttc accgttcctg
      301 gcgtttgaac gagcgccact acggggcgct gcaaggcctg gacaaggcag aaacggccgc
      361 gaagttcggc gaggaccagg ttaagatctg gcgccgcagc tacgatattc cgccgcccag
      421 cctggaaaaa agcgatccac gctatccggg caacgatcca cgctacagcg gcatacccga
      481 gcaggaactg ccactcgccg agtgcctgaa agataccgtc aaccggttca taccttattg
      541 gcacgaagtg attgcgcccg atgtgatgag cggacgccgc gtcatcattg cagcacacgg
      601 caacagcctt cgcgcgctgg tgaaatacct ggacaatatc tcggaccaag acattctcga
      661 gctgaacatc ccgacaggta tgccgctggt ttacgagctt ggagacaatt tgaaaccgat
      721 tcgaagctac tatctcggtg atcccgagaa ggtcaggaag gcgatggaag ccgtcgccaa
      781 tcagggcaag aagaaatagc ggcaatcagg gcggagcgcg gtctccgcca ccgctccctc
      841 gcggtcgcgg ctcggaaacc tttcatctcc ccagatctcg aacgtctacg gtgtgccggc
      901 ggaaggtgcg cgatacatcc cggcccagga tgtccgtggc cctcgtttca gcggcctgcg
      961 cctgactttt ttgcttcagt tttcggagca cgactgcgcg attgccccac agtaacccca
     1021 ggtcgggatg gttctgccct aacttttgtt cggctgtgcg gatcgctccg gcaagtaatt
     1081 cgtccgattc ctcgaaccgg cgttgatgaa accgcagcag cgcaagattg ttcacgatgc
     1141 ggatggtggc gggatgatcg ggccctgacg cctgacgcca gatctcgagc gccctttgaa
     1201 gtgccgcttc agccaaatca aactcctctt cagcttcata gagcacagac tggttgttca
     1261 ggatcactgc tacggagacg tggttgggcc cgatggcctg ttccgttaca gacttggctt
     1321 gctcgagaag tttggcggct tcttttttat ttccgtgcaa aaattccagc tctgagagat
     1381 tgttgtagat accggccaca tccgcgtgaa ttggaccgac ggtgcgaatc agcgcttcca
     1441 gcgattcgtg atatagggct tccgcgcgtt tatggtttcc cttcttgacc aacaagccgg
     1501 cgtagttgtt gaggagccag gcggagttct cccgctcctt ctccggaagc gccgcccgaa
     1561 tcttcaaagc ttcgcgataa cagcgctccg catcgttata tgctcccagg cgctcgcgcg
     1621 tggagccgag attgttcagt acggtgacgt gccggagatc ctgaggctta atgtctttca
     1681 atagccgcca cgcagtctcg agctgcgctt tggccgcttg ataatcgccg gcagcactaa
     1741 gtggcattgc ggcatcaaag tgctgtctcc aaacgctatc ggcaggtgcc ttgcgttcca
     1801 caggttcctg tgagagcgca tgacaaaata cgcccgatat tgtgaacagc agggccgctg
     1861 ctcccggtag tcgcattctg ttctctctct ggtgaaaaga gacggagcaa aatcaaaaaa
     1921 gttcgggcga ttgacgaaaa taataacgag gctatttctt cagagccgtc ccaaccaccg
     1981 tatgcggaaa ttcaacgcgc gccgctgtcc gccgtaatac cgcataaaat cggccgaagc
     2041 gatgttgctg ttgacggtgt cgtagtttct gtggttggtg acgttgttga gcccgccgcg
     2101 gatcgcgatc ttatggccca ttaacacgaa tcgccgctcc acgtggagat tcagctcgaa
     2161 gaattgggga aagcgctgag tgttcaatcc accggtgtag gcgccatcgg cgctgacaac
     2221 ggagaagggg aacccggttc tccattccag aagataagcc gccgcccagt tcttgaatgg
     2281 caggggcagg tacccccaac tgaggaaacg gtgcggcgta tcccatggca tcggcccggc
     2341 attgtcggag accaggatgg ggtcgtctat gttaacgtcc atgacggcgt tggatagcgc
     2401 ccttgaacgc acatagctgg ccatccattc atattgcccg gccaggacct gccgtatgga
     2461 aaactccacc gaatcgaaga catccttgcg catattgctg agggtataga ggctgtcgac
     2521 atcataggct tcgggcgccg ggatgaggta cggaacggca ttggacaatg gcttcccgcc
     2581 gccggtcaga tccatataag tcaagccttc ccgtccgcgt ttccgcagga gatcgacccg
     2641 caacgagaaa cccgacggca gttgttgcgc cacgccgagg ctccattggc cgtaccgcgg
     2701 cgaacgcaga ttgtaactgt cgattgtgta aagcgaaatg gcgggtccgc gaagaatgga
     2761 accatccgga ttgaaagtag tcgccaacga gtactgatcc agcggcctgg tgaagagacg
     2821 gaggttggtt gcctcgtata cgatggcgta gccgccggag atcttggtat tctcccatcc
     2881 ttttggcgac caggcgaatg ccgcgcgggg agagaaatta tagttgtgca ggatagagtc
     2941 ccagtcactt cgcagaccca gttccagcac gatgccgggc cgcacacgcc aggtgtcctg
     3001 tacgtaagcg gccgcctccc aggcggaccg gacgaacgcg ccgtttcctc caaaaacagt
     3061 ccgcctcagg ggaatgccgt ccggccggag atattcgata ccggtgcggt ggacattctg
     3121 ctggtattga agtctgttga tatcgattcc ggtt
//
LOCUS       DASTMK010000536        16043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_60506, whole genome shotgun sequence.
ACCESSION   DASTMK010000536 DASTMK010000000
VERSION     DASTMK010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16043
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60506"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFQ79_22200"
     CDS             complement(<1..461)
                     /locus_tag="VFQ79_22200"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU0142450.1"
                     /translation="MQELAALFVSWGPFGLFLVALLDSAGIPLPAGVDALVVTLSVMR
                     PERAYFLAILAVIGSLIGSMFLYYLARRGGQRYLNRYTISGRGAKFNAWFLKYGLITV
                     FVPAFLPIPLPLKVFVICAGALGVRPSIFLVVMLTARIPRYFGLAILGAELG"
     gene            complement(461..2452)
                     /gene="ligA"
                     /locus_tag="VFQ79_22205"
     CDS             complement(461..2452)
                     /gene="ligA"
                     /locus_tag="VFQ79_22205"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU0142451.1"
                     /translation="MPTPASREIERLREELRRHEYLYYVMDEPEIGDAEYDGLMRRLH
                     ALEEEHPDLVTPDSPTQRVGGKPREGFQQVPHLSPMLSLDNALNETELKDFDRRVRDG
                     LRGEKYCYTAELKMDGLSMSVRYENGAFTQAVTRGDGLVGEDVTENARTIRSLPLRVT
                     GDAALERFEVRGEVIMTHRAFERLNEERDGQGLPRYANPRNSAAGSLRLLEPWITASR
                     KLDFFAYFLLVDGQPYFPSHAESLSKLKSMQFKVNPHWKVCGGIEELLEFCRVWEAKR
                     DELPYEIDGVVAKVDSIAQQKKLGRTAKAPRWAIAYKYAARQQTTVVENIEVQVGRTG
                     TLTPVARLRPVIVGGVTVTRATLHNEDEIERLGVEIGDTVLVERSGDVIPKVVKVTEK
                     SPNATRFRMPSHCPVCGGEIVRAQGEAASRCVNTNCPARLKESVLHFSARGVMDIDGM
                     GEALVDQLVGRGMVRSVADLYELTSEKLLELERMGQKSAQKVIENIHNSKKRPLPRVI
                     NGLGIPFVGERTAEILAETFGSLDEIARAGEEELQSAQEVGPKVAQSIRRFFEEPRNQ
                     ELVERLRAAGLHFEHQRKRKAGGPLEGLTFVLTGTLPTLTREEAKAKIEEAGGKATGS
                     VSKKTDYVVAGEEAGSKLEKAQKLGVKVLDESELLRMIG"
     gene            2446..3375
                     /locus_tag="VFQ79_22210"
     CDS             2446..3375
                     /locus_tag="VFQ79_22210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU0142452.1"
                     /translation="MASTYNVGIVIYKNTILIYNPTAGALKKGDQKLRRAVQVLADRG
                     FEVQLRPTTGPFTATLIAREAVQEGADLIIAAGGDGTINEIANGMVGSAVPLGILPAG
                     TANVLANELRLSSKMEAAAAALPASTPRRISVGRLNAEGRSRYFLMMAGVGFDAQIVY
                     NLNLDLKRALGKIAYWIAGFRMAAARVAQFETRWNDTSMQGGFALAARVRNYGGDLEI
                     ARGACLLGHDFELVVFEGDRAITYVKYLAGVLTRSAHRLRGVTITRTRCAGFYSTPAD
                     SRVYVQVDGEYAGRLPAQVEIVENALTLLMPPG"
     gene            3426..4526
                     /locus_tag="VFQ79_22215"
     CDS             3426..4526
                     /locus_tag="VFQ79_22215"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0142453.1"
                     /translation="MQRGPLIFLTIVTLVLLPFVWQIARPFLTPFLLAAILAIVMGPV
                     KESLERHLGRPALATVLTTLAAVLIFGAIVVFTGMALTRELGAAYSELNEQSIEQGGW
                     TPLVTHTTDRVLTVLEDWLPLNREQIRAEMIARLKALTGLLLKMIGPVVEGVVSFIVT
                     GFFIALFLYFLLRYGKAWVQEVAELLPLEHATTTSLFQTIEDAIVANVNGVMAVAVAQ
                     GILLGVGFWLVGLHSPVLWGVIGGLASVIPMIGSLLVWVPIVIAFLAMGLYWKAILLA
                     AWCAVLVGSVDNVVRPLVVGGRIRQHPVLIGLAMIGGASAFGALGILLGPVIVSLLIA
                     LIEEIQSVRASSNAPLTTPTTTTSPVEEHPRA"
     gene            complement(4537..4995)
                     /gene="fliS"
                     /locus_tag="VFQ79_22220"
     CDS             complement(4537..4995)
                     /gene="fliS"
                     /locus_tag="VFQ79_22220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEU0142454.1"
                     /translation="MINNQFDAYLEGEILSADPVQLVRILYRAAIEATGQARQCLREG
                     DITGRGRAIAKASAMLNELNLSLNYEAGGEVSRNLAELYDYLQRRLMTANFEQVDPPL
                     IEVENLLFTLLDGWAHPAESEPEIDSAGLVPEALFASAAAAEYQPVCYSF"
     gene            complement(5051..5338)
                     /locus_tag="VFQ79_22225"
     CDS             complement(5051..5338)
                     /locus_tag="VFQ79_22225"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HEU0142455.1"
                     /translation="MEIGRTQLNVHSPVLPATPLLSDDLAARKQLIQAVKAVNTAELL
                     GENRELTFSLDRESRRTILRIVDRKTKEVVKQIPPEYVLRLAEDLKRGVFA"
     gene            complement(5429..5974)
                     /locus_tag="VFQ79_22230"
     CDS             complement(5429..5974)
                     /locus_tag="VFQ79_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU0142456.1"
                     /translation="MPENFEISQPAELFARIDNENAEPLSDGVLLTTVDKAINWARKN
                     SIWPMTFGLACCAIEMMAMTASRFDIARFGAEVFRGSPRQSDLMIIAGRVANKMAPVI
                     RQLYQQMPEPKWVISMGACATSTGVFNNYALIPVNQVIPVDVYVPGCPPRPEQLIYSL
                     TLLQKKIEKESGTLRKVLNLA"
     gene            6043..8301
                     /gene="malQ"
                     /locus_tag="VFQ79_22235"
     CDS             6043..8301
                     /gene="malQ"
                     /locus_tag="VFQ79_22235"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU0142457.1"
                     /translation="MPVRSLSFHSHSTYEESLLLAAKLWGVEPDFWDIWGNYHAIPAE
                     VQRAILESMGVEAGSAETLNRIISGRLQREWGSYTPQTLVVSENSAHFPVRLPEAQSE
                     ISLVFEVRLEDGTTRQWQAPAAGAETSATAEIEGRRYIEKQVAAEKLPLGYHHLTVRA
                     EVDGVESATRLIVCPDRTYTPPDLSDGGKAAGIAVALYGLRSERNWGCGDFTDLHGLI
                     DWAAEDAGCSLVALNPLHAIPNRAPYNTSPYLPNSSYYLNFIYLDVERLEDFQKSECA
                     RRLMQSPGVRNEIQALRDSQYVEYERVASLKLRMLKTAFRRFLREEFRRDTHSAKRFQ
                     EFVEKEGVLLDRYAVYYALDEWQHKKNPDVWVWRQWSEEYQSPESPAVRDFAKQHWRS
                     VLFYKYIQWQIDAQLREAQQYAQSKGMPVGLYHDLALATDSYGSDLWAYGRFYVNGCR
                     VGAPPDDFSPNGQDWGFPPPNTDQHRRDGYRLFTESIRKNARHGGALRIDHVMRFFHL
                     YWIPENQDARNGAYVQDYYDDLIRILALESVRNQFVVIGEDLGTVADYIRETLERFGV
                     LSYRLLYFEKDKGGNFLPPDRYPVQALVSVSTHDLPTLAGFWSGRDIEARREVGLIDE
                     AARSRQMEERQIEKQKILDLLFQFHLLPEPYPTTAADLPELTGELHNAVAGFLASTPS
                     RLMVLNQEDLFKDGEQQNLPGTTHQYPNWRHKMMFRVEELRQSRQACDYTRMFRNWLA
                     ATGRLRTGQGTV"
     gene            complement(8275..9156)
                     /locus_tag="VFQ79_22240"
     CDS             complement(8275..9156)
                     /locus_tag="VFQ79_22240"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0142458.1"
                     /translation="MRDRIRLAAVEFDAGTASTEENLEKLAAWCGRAHEQGAELALFP
                     ELSVTGFIPNHPEGEHSAWLREALRGARRMAERLNGCAVRRLSEIAAHTGMMIAAGML
                     EDAGNVLYNTHVLVGPDGLLGAWRKLHIPIFEMPLYNGGPAPPVVETPLGKIGINICF
                     DAFLPESTRLLAVQNAEIVLFPFAADPPPPGPAGWEQWAEPVLRARCAENGVFGLASN
                     YTGEVSFAGVQQRFYGGALALGPDGNVLARGARQSSEPRMLVVDFDRELLDCARASFE
                     YTFRFRRPELYGSLARS"
     gene            9339..10310
                     /gene="pfkA"
                     /locus_tag="VFQ79_22245"
     CDS             9339..10310
                     /gene="pfkA"
                     /locus_tag="VFQ79_22245"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU0142459.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVIRTSIGNGWEAFGVRNGFAGL
                     VAGTFTPMGSRDVSEIMQRGGTILGSARAPQFAEENVRRQALHHLAANGIEALVVIGG
                     NGSQTGAHALSAMGFPVVGVASTIDNDLYGSDVTLGVDTALNIALEAIDRLKVTASSH
                     QRAFLVEVMGRDCGYLALMAGIAGGAEAIVIPEVDLHPDKIAEEVRAAHERGKPHVLI
                     VVAEGAHYNGEALKEYMIENRSRVGFQFRLTILGHVQRGGTPTFADRMLGTRLGAAAV
                     EQIAKGNTGVLAGVQGGQISTTPLSEVVVRKKPLDMSLLELAGVLAM"
     gene            10365..11687
                     /locus_tag="VFQ79_22250"
     CDS             10365..11687
                     /locus_tag="VFQ79_22250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0142460.1"
                     /translation="MNRRTFLASTAAAAGPAQPAQKLAINGGTPVRTTPLAARHFGPL
                     YYDEKERAELLEVLETRRPFRWWGAGKEPPAKVANFEKEFAAFMKVRYALAVTSGTAA
                     LECSMAALGIGPGDEVILPSWTWHSCASSVIRAGALPVFAEIDESFNIDPSDIEHRIT
                     PATKLIMAVHLQGNPADMDRILAVARKHRIRVLEDCAQSVGGSYKGRPLGSIGEIGIF
                     SLQLSKTITAGEGGAVVTSDPLLFERAARFHDTGAIRKPHEDWIGEVRDEGFAGTNFR
                     MNEFSGGVLLAQLRKLGMIVNSCRANARRVYDGIRDLPGARLRTLPDPSGEIGSAVFL
                     GFPNKDRRDRFVEAMTKENVPARPPGGSVILPVQKYIMAKRTPHPAWPSFTSERGRAI
                     QYGPAACPRTIDILDRFAGVSIDPKFTKADTDDIVAAIRKVYPALSAA"
     gene            complement(11663..12529)
                     /gene="lipA"
                     /locus_tag="VFQ79_22255"
     CDS             complement(11663..12529)
                     /gene="lipA"
                     /locus_tag="VFQ79_22255"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU0142461.1"
                     /translation="MLVTLETTPQRPKWLRAPAPGGENYRELKTLVDELRLHTVCESA
                     ACPNVGECWNRRTATFMILGNVCTRRCGFCAVQKGGPLPVDYDEPRRVAEAVAALGLR
                     YAVVTSVNRDDRKDGGAELFKLTIEAIRHRVAGCRVEVLIPDFQGSQAAMELVMSAAP
                     DVLNHNTETVPRLYRQVRLGARYERSLEMLAYAKKCRPEIPTKSGLMLGLGETRDEVL
                     QVMRDLRAHDVDILTLGQYLRPSPKHLPVIRYVPREEFDQYRIDGLEMGFRHVESGPL
                     VRSSYHAAESAG"
     gene            complement(12536..13951)
                     /gene="ftcD"
                     /locus_tag="VFQ79_22260"
     CDS             complement(12536..13951)
                     /gene="ftcD"
                     /locus_tag="VFQ79_22260"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEU0142462.1"
                     /translation="MARQLVECVPNFSEGRNRDVVEAVADAILSVPGTFVLSRELDPD
                     HNRSVITFVGAPEAVGEAAVRAVGKAAELIDLTAHNGVHPRIGAADVVPFVPVEGVTL
                     DDCVRIARWTGEQIWARWNVPVYFYEAAATKPRNTNLENIRRGNFEGLSSAVMTDAER
                     VPDVGGPALHPTAGATVVGARKFLIAFNINLGTSDLEIAKHIARAVRFSSGGLRYVKA
                     LGLALESRGIVQVSMNLTDFEQTPIHRVFEMVSLEASRFGVPIVGSELIGLVPKKAIE
                     MTAEHYLRFESFHTHTILENRLADVAASETGLHDFLDALAAPTPAPAGGSASAAAAAM
                     AAALGAMVARMADQPDAVFEDARRLLQEAVTRDSAAYEAVVSARKRPGAEREKHLQEA
                     LQKATLVPLEVMECTRGLQLEIEKLEAAAPPRFAPEMETAKALAQATLIGALASVTAN
                     IPLVKDATFVAGIEERLRALK"
     gene            complement(14022..15290)
                     /gene="hutI"
                     /locus_tag="VFQ79_22265"
     CDS             complement(14022..15290)
                     /gene="hutI"
                     /locus_tag="VFQ79_22265"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU0142463.1"
                     /translation="MTQTKYLLIRGARQLLTLRGPSGPRRGSALRDLGIIEDGSVLIS
                     NGVVVEVGTSRRVENLAESRKAHVIDASGQVVMPGFVDSHTHLISGTPRMTDHEMRLA
                     GAGEDAIRKAGGGFDATMREVRRNSARRREFWAAKAIRAFVRHGTTTIEAKSGYLLDQ
                     AGELKCLRTFNSLNGTIADIEPTYFGAHRVPPESSGSCEYLAWICAEMLPVIRSRKLA
                     RFVDIHCGPDGFSPEQCRKYLACAKQQGFLLKVHAQTKTHDEGVCVAVEAGAVSVDHL
                     DYANTVDAALLARSNTVAALTPGILLGKTGHLPPARELIDHGAAVALATGFDSSIACT
                     SNMQAVIAFACAQLQMTPAECVSAATVNGAHALRRAGQVGSIEYGKQADLLILDASDY
                     RELSYWCGTNLVAITIRKGVPIYRAGEVVV"
     gene            15383..>16043
                     /locus_tag="VFQ79_22270"
     CDS             15383..>16043
                     /locus_tag="VFQ79_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0142464.1"
                     /translation="MDRRFQTDLIQSPPAGRWDFLAKPRRFLSPIFRYWMETEVHVYG
                     LSIGASVLLSFFPFLIVMVSLCRYALQWPAAEGAIYLALEDYFPGEMGNFIRRNLRAT
                     VASRGPFQVISVLLLLFTANGIFEPLEVALNRVWGCAKNRSYVRNQLVSLGLIFACGT
                     LAMISMILTAMNQTFLTRLVGTNTVTAAFLGGVLFKMAAIPISILILLLVYWLLPNCK
                     VP"
BASE COUNT         3100 a         4838 c         4785 g         3320 t
ORIGIN      
        1 cccagttcgg cgccgaggat tgccaggccg aaataccgtg gaatgcgcgc cgtcagcatc
       61 acaaccagga agatcgacgg acgcacgcca agagccccgg cgcaaatgac gaacactttg
      121 agaggcaacg ggatcggaag aaaggcaggc acgaacacgg taatcagacc atatttcaaa
      181 aaccatgcat taaattttgc gccgcgcccg gagatcgtgt accggttcag atacctctgg
      241 ccgccacgcc gggccagata atacagaaac atcgacccga tcagcgatcc aatcaccgcc
      301 aatatcgcca gaaaataggc tctttccggg cgcatcaccg acaaggtcac cacgagcgca
      361 tccaccccgg ccggcagtgg aatgccggcg gagtctagca atgcgaccag gaacagaccg
      421 aatggtcccc aggagacaaa taacgcggca agttcttgca tcacccgatc attcgcagca
      481 gttcggattc atccaacacc ttcacgccta gcttttgcgc tttttccagc ttcgagccgg
      541 cctcttcgcc cgccacgacg tagtctgttt ttttactgac ggacccggtc gccttgcctc
      601 cggcctcttc gattttcgct ttggcttcct cgcgggtcag cgtgggcaga gtacctgtca
      661 gcacaaatgt aagcccctcc agcggtcccc cggctttgcg ttttcgctgg tgctcgaagt
      721 gcaatcccgc ggcccgcaac ctctcgacca gttcctggtt ccggggttcc tcgaagaacc
      781 ggcggatgct ctgggccacc ttaggaccca cttcctgcgc cgactgtaat tcctcctcgc
      841 ctgcccgggc gatttcatcc aggcttccga acgtttccgc caggatttcg gccgtccgct
      901 cacccacaaa tggaatgccc agcccgttga tcacccttgg gagcggccgc tttttcgaat
      961 tgtggatatt ttcaattacc ttctgcgcgg acttctgccc catgcgttcc agttcgagca
     1021 atttctccga ggtcaactcg tacaggtccg ctacactcct caccatgcca cggccgacaa
     1081 gctggtccac cagcgcttcg cccatgccgt cgatatccat gacgcctctg gcggaaaaat
     1141 gcagcacgga ttctttcaag cgggccggac agttcgtgtt tacgcaccgg ctggcggctt
     1201 ccccctgcgc ccgtacgatt tctccgccgc agaccgggca gtgcgacggc atgcggaaac
     1261 gcgtcgcgtt cggagatttc tccgtcacct tgacgacttt gggaatgaca tctccgcttc
     1321 gctccaccag cacggtgtcg ccgatttcga cgccgagcct ttcaatctcg tcctcattgt
     1381 ggagagtcgc ccgcgtaacg gtgactcctc ccacgatgac ggggcgcaat cgtgctaccg
     1441 gcgtcagcgt gccggtacgc cccacctgta cctcgatatt ctcgacgaca gtggtctgct
     1501 ggcgagcggc atacttgtaa gcgatagccc accgcggcgc tttggccgtc cggccgagct
     1561 tcttctgctg cgcgatggaa tcgactttgg ccacaacgcc atcgatctcg taaggcaatt
     1621 cgtcgcgctt tgcttcccac acccggcaaa attcgagcag ttcttcgata ccaccgcaca
     1681 ctttccagtg tggattcacc ttgaattgca tgctcttgag cttgctgagc gattccgcgt
     1741 ggcttggaaa atacggctgg ccgtccacaa gcagaaaata ggcgaaaaag tcgagtttgc
     1801 gcgacgcggt gatccacggt tcgagaagac gcaacgaccc tgccgccgaa ttgcgagggt
     1861 tggcatatcg cgggagaccc tgcccgtcgc gctcttcgtt caggcgctca aaagcgcggt
     1921 gggtcatgat gacttctccc cgcacctcga agcgttcgag ggccgcgtcg cccgtcacgc
     1981 gcaacggcaa ggagcgaatc gtgcgggcgt tttccgtaac atcctctccc accaggccat
     2041 cgccgcgggt gactgcctgt gtgaacgccc cgttctcgta acggacagac atcgagagtc
     2101 cgtccatttt tagctccgcc gtgtagcagt atttctcgcc tcggagtccg tcccggacac
     2161 ggcggtcgaa atctttcaac tcggtctcat tcagcgcgtt atcgagactc aacatcgggg
     2221 agagatgcgg tacttgctga aatccctccc gcggcttgcc gccgacgcgc tgtgttgggg
     2281 aatccggcgt taccaggtcc ggatgctctt cttccagggc gtgtagccgg cgcatgagac
     2341 cgtcatactc ggcatcgccg atctccggct catccatcac gtagtacaga tactcgtggc
     2401 gccgcagctc ttcccttaac cgttcgatct ctctgctggc tggtgttggc atcaacttat
     2461 aatgtgggta tcgttatcta taagaataca atcctgatct acaatcccac cgcaggtgcg
     2521 ttaaagaaag gcgatcagaa actaagacgc gccgtccagg tacttgccga tcggggtttt
     2581 gaagttcaat tgcggccaac caccggaccc tttaccgcca ctctgatcgc tcgcgaggcc
     2641 gtccaggagg gcgccgattt gatcatcgcc gccgggggag acggcaccat caatgagatc
     2701 gctaacggca tggttgggtc ggctgtgccg ctgggaatcc tgccggcggg cacggccaac
     2761 gtgcttgcga atgaactgcg gctgagctcg aagatggaag ccgccgcggc tgcgctgccc
     2821 gcatcaacgc cgcgccgcat ctccgtgggc cggctgaacg cggagggacg cagccggtat
     2881 ttcctgatga tggcgggtgt gggtttcgac gcgcagatcg tatataactt gaatctcgat
     2941 ttgaagcgtg cactggggaa gatcgcctat tggatagccg ggtttcgtat ggctgcggcg
     3001 cgcgtggcgc agttcgaaac acggtggaac gacaccagta tgcaaggcgg gttcgctctc
     3061 gccgcgcggg ttcgcaacta tggcggagac ctggagatcg cccgcggtgc ctgtttactc
     3121 ggacacgatt tcgaactggt cgtgtttgaa ggcgatcggg caataaccta tgtgaagtat
     3181 ctggcaggcg ttctgacccg atccgcacac cgcctgcggg gcgtaaccat cacgcgaacg
     3241 cgttgcgccg gattctacag caccccggcg gattcccgcg tgtacgtcca ggtggacggg
     3301 gaatatgcgg gccgcctgcc cgcacaagtc gagatcgtcg agaacgcgct gacgctgttg
     3361 atgccgccgg gctgatgatg ccggtatgct attctcgatt tgactgaacg ggagtattgc
     3421 cgcacgtgca acggggtcct ctgatttttc tcactattgt gacgctcgta ctgctgcctt
     3481 tcgtgtggca gattgcgcgg cccttcctca cccccttcct gctggcggct atccttgcca
     3541 tcgtcatggg accggtaaag gaatcgctcg agcgccatct cggccggccg gcgctggcca
     3601 ccgtactgac tacgctggcg gccgttctca tttttggcgc tatcgtcgta ttcaccggga
     3661 tggcgctgac gcgggaactg ggcgcagcgt actccgaatt gaatgagcaa tcgatcgaac
     3721 aggggggatg gacgccgctg gttacgcaca ccacggatcg cgttttgacc gtgctcgaag
     3781 actggctccc cctgaatcgc gagcagatcc gcgcggagat gatcgcccgc ctgaaagcgc
     3841 ttaccggttt gctgttgaaa atgatcggac cggtggtgga aggggtggtc tccttcatcg
     3901 ttacggggtt ctttatcgcg ttatttctgt attttctgct tcgttatggc aaagcctggg
     3961 tgcaggaggt ggcggagttg ctgccactgg agcatgcgac cacaacgagt ctcttccaga
     4021 ccatcgaaga cgccattgtt gccaatgtga acggcgtgat ggccgtagcg gtggcgcaag
     4081 gcattctttt aggggttgga ttctggctgg tcggactaca ctctcccgta ctttggggtg
     4141 ttatcggcgg cctcgcctcc gtgattccga tgatcggcag cctgctcgtc tgggtgccca
     4201 ttgtgatcgc ttttttggcg atgggccttt attggaaagc gatcctgttg gcagcctggt
     4261 gcgccgtgct ggtagggtcg gtggataacg tagtacggcc gctggttgtg ggcggccgga
     4321 tccggcagca ccctgtgctg atcggcctcg cgatgatcgg gggagcgagc gccttcggcg
     4381 cgctcggcat tctactcggc ccggtaattg tctcgctgtt gattgccctg attgaagaga
     4441 ttcaaagtgt gagggcgtcg agcaacgcac cgttgacaac gcctacaacc actacttcgc
     4501 ctgtggaaga acatccaagg gcctgacgtt cccacgttag aacgaatagc acaccggctg
     4561 gtattcggct gccgcggccg aggcgaacag cgcttccgga accagtccgg cagaatctat
     4621 ttcaggttcg gattcagccg ggtgcgccca gccgtcgaga agcgtgaaca gcagattttc
     4681 cacttcgata agtggtgggt ccacctgctc gaagttggca gtcatgaggc ggcgctgaag
     4741 gtaatcgtag agttccgcca ggttccggct cacctctcca ccggcttcgt agttcaggga
     4801 caggttcaat tcattgagca tggccgatgc cttggcgatc gcccgtccgc gtccggtaat
     4861 gtcaccttcc cgtagacact ggcgagcttg tccggttgct tcaatcgcgg cccggtaaag
     4921 gatgcgtacc aattgcaccg ggtctgctga aaggatctcg ccctccaggt aagcatcgaa
     4981 ctggttattg atcatcaatc atcgcctcaa aaaccttatc ggctgcggag gcgggttctt
     5041 ggacacaact ttaagcgaat actcctcttt tcagatcctc ggcgaggcgc aggacatact
     5101 ccggcggaat ctgtttcacg acttccttgg tcttgcgatc gacgatacga aggatcgtac
     5161 ggcggctttc gcgatccagt gaaaacgtca actcccggtt ttctcccagc aattcagcgg
     5221 tgtttaccgc cttgaccgcc tgaatgagtt gctttctcgc tgccagatcg tctgataaca
     5281 agggtgtcgc cggtaacacc ggcgagtgga cattgagttg tgtacgaccg atttccatgg
     5341 caacctccga ggaggataga actcgcctcc tcaaaaagct catcggtcgc gagcgccgga
     5401 gttttagtaa aaatgagccg cgcggctctc aggccaggtt caaaactttc cgcagggttc
     5461 cggattcctt ctcgatcttc ttctggagca gcgttaacga ataaatcaac tgctccggcc
     5521 ggggcggaca gccgggaacg tagacatcca ccgggatgac ctggttcacg gggatgagtg
     5581 cgtagttgtt gaaaacaccc gtcgaagtcg cacaggcgcc catcgatatg acccatttgg
     5641 gttccggcat ctgctggtag agctgccgta tcaccggcgc cattttgttc gcgacgcgtc
     5701 cggcgataat catcaggtcg gactgccggg gagatccacg gaacacctct gctccaaaac
     5761 gcgcaatgtc gaatcgcgaa gccgtcatcg ccatcatctc gatggcgcaa caggcaaggc
     5821 cgaaggtcat cggccagatg gaatttttgc gcgcccagtt gatggccttg tctaccgtag
     5881 ttaacaggac gccatcggat agtggttccg cattctcgtt gtcgatccga gcgaaaagct
     5941 cggcgggctg gctgatctcg aaattttccg gcatcgcctc tctcttcggt ctattatccc
     6001 atgcctgaaa cggaacctca gccggtacaa tccattagag agatgcctgt ccgctcgctg
     6061 tccttccata gtcattcaac gtatgaagaa agcctgctct tagccgcgaa attgtgggga
     6121 gtcgagccgg atttctggga catttggggg aattatcacg cgattcccgc cgaagttcag
     6181 cgggccattc tggaatccat gggggtcgaa gcgggcagcg cggagacctt gaaccggatt
     6241 atttccggcc ggctccaacg cgaatggggt tcctataccc cgcagacgct cgtcgtcagc
     6301 gagaacagtg cgcattttcc cgttcgcctc ccggaagccc agtcggaaat ctccctcgtt
     6361 ttcgaggtgc gcctggagga tggcaccact cgtcaatggc aggccccggc ggcgggcgcc
     6421 gagaccagcg cgaccgccga aatcgaagga cgccggtaca ttgaaaagca ggtcgcggcc
     6481 gaaaaacttc cgcttgggta tcatcacctt accgttcgag ccgaggtgga cggtgttgaa
     6541 tccgccacgc gcctcatcgt ctgtcccgac cggacataca ccccacccga tctctcggat
     6601 ggggggaaag cagcgggaat tgccgttgcc ctttacggtc tccggtccga aaggaactgg
     6661 ggctgcggag acttcacgga cctgcacggc ctcatcgatt gggccgccga ggatgcggga
     6721 tgcagcctcg tcgcgctgaa ccctctgcat gccattccaa accgcgcgcc gtacaacacc
     6781 agcccgtatc ttcccaactc cagctattac ctgaatttca tctatctcga cgtcgaacgc
     6841 ctggaggatt tccagaaatc ggagtgcgcc aggcggctga tgcaaagccc gggcgtgcgg
     6901 aacgagattc aggctctgcg cgattcgcag tacgtggaat atgaacgggt tgcgtccctg
     6961 aagctgcgga tgctcaaaac cgcattccgc cgctttctgc gcgaagagtt ccgccgggat
     7021 acacactctg ccaaacgctt tcaggagttt gtagagaagg agggcgtgct gctcgaccgt
     7081 tatgccgtct attacgccct ggacgagtgg cagcacaaaa agaaccccga tgtgtgggtg
     7141 tggcggcaat ggtccgagga gtatcagagt ccggaatcac ccgccgtgcg ggacttcgca
     7201 aaacagcatt ggcgcagcgt gctgttctac aaatacatcc aatggcagat cgacgcgcag
     7261 ctacgcgagg cgcaacaata cgcgcagtcg aagggcatgc cggtgggact gtaccacgac
     7321 ctggctctgg ccaccgacag ctacggctcg gacctgtggg cgtacgggag attctatgta
     7381 aacgggtgcc gcgtcggagc gccacccgat gatttctctc ccaatggcca ggactggggc
     7441 tttccaccgc ccaacacgga ccagcaccgc cgcgatggct accggctctt caccgaatcc
     7501 atccgcaaga acgcgcggca tggcggtgcc ttgcgaatcg atcacgtgat gcgcttcttt
     7561 catctttatt ggattccgga gaaccaggac gcgcgaaacg gagcttatgt tcaggactat
     7621 tacgacgacc tgatccgtat tcttgctctc gagagcgttc gcaatcagtt tgtcgtcatt
     7681 ggggaagacc tgggaaccgt cgccgactac atccgcgaga cgctggagcg cttcggcgtg
     7741 ctcagctacc ggttgctcta cttcgaaaag gacaagggag gaaattttct tccgccggat
     7801 cgctatcctg tacaggcgct ggtgtcggta tcgacgcacg atttgccgac gctggcgggt
     7861 ttctggtcgg gacgcgacat cgaggcccgg cgcgaagtgg ggcttatcga cgaggccgca
     7921 cgctcccgtc aaatggaaga gcggcaaatc gagaagcaaa agatcctgga cctgctgttt
     7981 caattccatt tgttgccgga gccgtatccc accaccgcgg cggaccttcc ggaactgacg
     8041 ggagagttgc acaatgccgt cgccgggttt ctggcttcaa cgccctcgcg gttgatggtt
     8101 ttgaaccagg aggacctgtt caaagacggc gaacagcaaa acctgcctgg cacaacacat
     8161 caatacccga actggcggca caagatgatg tttcgcgtag aggagctgcg ccagtcgcgc
     8221 caggcatgcg attacacgag aatgtttcgc aattggctgg cggcaaccgg ccggctacga
     8281 acgggccagg gaaccgtata gttccgggcg gcggaaacgg aacgtgtatt cgaagcttgc
     8341 acgggcgcaa tccaacagtt cacggtcgaa atcaacgacg agcatgcgcg gttcgcttga
     8401 ttgccgagcg ccgcgggcca gcacgttccc gtccggcccg agcgccagcg cacctccgta
     8461 gaaacgttgt tgaacaccgg cgaacgagac ctcgccggtg taattcgagg cgagtccaaa
     8521 cacgccgttt tcggcgcacc gcgcacgcag cacaggctcc gcccattgct cccatccggc
     8581 gggaccgggc ggcggcgggt cggcggcgaa ggggaaaagg acgatctcgg cattctgcac
     8641 cgccagcagc cgggtcgatt caggcaggaa cgcatcgaaa cagatattga tgccaatttt
     8701 tccaagcggc gtttcgacga caggaggagc cgggccgccg ttgtacaggg gcatctcgaa
     8761 aatcggaata tgcaatttgc gccaggctcc cagaaggccg tccgggccca ccagaacatg
     8821 cgtgttatag agcacgtttc cggcatcctc caacatgccg gctgcgatca tcatgccggt
     8881 gtgggcggcg atttctgaca gccggcggac cgcgcatcca ttcaaacgtt ccgccatgcg
     8941 gcgcgcgcct cgcagggctt ccctcagcca tgcggaatgc tcaccctcgg gatgattggg
     9001 gatgaatccg gtgacgctca gctccggaaa caacgccagc tcggcgcctt gttcatgcgc
     9061 acggccgcac cacgccgcca gtttttcgag attttcttcc gttgatgccg tacctgcgtc
     9121 gaactctacc gccgccagcc gaatgcgatc ccgcatgggg tgttccgcct cctctccgta
     9181 tttaatcggg taggagccgt caaccgcaat gcgggaccgc ttgctggcgc gcgcggctca
     9241 gtaagtcctt tgtttctgag ccgcgacggt cacggagcgg ttgcgcgtat cgaacgaata
     9301 tacggcgaat tttggagaca gaacactaga atgaaggagt gaaaaggatt gctgttttaa
     9361 ccagcggcgg cgacgctccg ggaatgaatg ccgccattcg ggcggtgata cggacatcga
     9421 taggcaacgg gtgggaggcc tttggagtgc gcaacgggtt cgccggcctt gtcgccggca
     9481 cgttcacgcc gatgggtagc cgggatgtga gcgagatcat gcagcgcggc ggcaccattc
     9541 tcgggagtgc ccgtgcccct caattcgccg aagagaacgt gaggcgccag gcccttcacc
     9601 atctggctgc gaatggcatt gaagcgctag ttgtaattgg tggcaacgga tcgcaaaccg
     9661 gggcgcacgc cctctcggcg atgggcttcc ccgtcgttgg tgtcgcttcc acgatcgata
     9721 acgatctgta cggctccgac gtcacgctcg gcgtcgatac cgcgctaaac attgcgctcg
     9781 aagccataga ccggctgaaa gtgacggcct cctcgcacca gcgggcgttt ttggtcgaag
     9841 tcatgggacg ggactgcggc taccttgccc ttatggccgg gattgcgggc ggcgcagagg
     9901 caatcgtgat tccggaggtt gacctgcatc cggacaagat cgccgaagag gtccgggcgg
     9961 cgcatgagcg tggaaagcca cacgtcctga ttgtggtggc cgagggcgcg cactacaacg
    10021 gcgaagccct gaaagaatac atgattgaaa atcgcagccg cgtgggcttc cagttccggc
    10081 tgacgatact gggccatgtg cagcgcggcg gaacgcctac tttcgccgac cgcatgctcg
    10141 gcacgcggct gggcgcggct gcggtggagc agattgcaaa gggcaacaca ggtgtgctgg
    10201 ccggtgtgca gggcggccaa atctccacaa ccccgttgag cgaagtggtg gttcgaaaga
    10261 agccgctcga catgagcttg ttggagcttg cgggcgtgct cgcgatgtag cgattgccgg
    10321 ggccgcgcgt cgggcctatg ctggtgtgga caaggagcgc gttcatgaac cgaagaacgt
    10381 ttctggcttc caccgccgcg gctgccggac cggcgcagcc tgcacagaag ctggctatca
    10441 atggcggaac gccggttcgc accacaccgc tggcggcccg ccatttcggc ccgctatatt
    10501 acgacgaaaa ggaaagggcc gaactcctgg aagttctcga gacaaggcgt ccgttccgct
    10561 ggtggggtgc ggggaaagag ccgccggcga aggtcgctaa tttcgagaag gagttcgccg
    10621 ctttcatgaa ggtgcgctat gcgctggctg tcacatccgg gacggcggcg ctggagtgct
    10681 cgatggccgc cctcggtatc ggaccgggag atgaagtgat cctgccctcc tggacctggc
    10741 actcctgcgc aagttcggtg atccgcgccg gcgcgttgcc ggtcttcgcg gagatcgacg
    10801 aatcgttcaa tatcgatcct tccgatatcg aacaccggat cacgcccgcc acgaagttga
    10861 ttatggcggt ccatcttcag ggcaatcccg ccgatatgga ccgcattctt gcggtcgcac
    10921 gcaaacatcg gattcgtgtg ctcgaagatt gcgcgcagag cgtgggaggc agctataaag
    10981 ggcgtccgct gggttccatc ggggagattg gcattttcag tcttcaactg agtaagacca
    11041 tcaccgcggg cgagggaggc gcggtggtga cgagcgaccc cctgctgttt gagcgggccg
    11101 cgcggtttca cgataccggc gcgatccgca agcctcacga agactggatt ggcgaggtcc
    11161 gtgacgaagg gtttgcggga acgaacttcc ggatgaacga gttcagcggc ggcgtgctgc
    11221 tggcccaatt gcgaaagctc ggcatgattg tgaactcctg ccgcgccaac gcacggcgag
    11281 tctatgacgg gattcgcgat ctgcccggag cgcgtttgcg cacgctgccc gacccgtccg
    11341 gcgaaattgg ttccgcggtc tttctcggct ttccgaataa agaccggcgt gaccgtttcg
    11401 tggaggctat gacgaaagag aacgtgcctg ccaggccgcc gggcggatcc gtgatcctgc
    11461 cggttcaaaa atacatcatg gcgaaaagaa cgccgcatcc ggcatggccg tcgttcacgt
    11521 ccgaacgggg ccgggccatc cagtatggac ccgcggcttg tccccggacc atcgatattc
    11581 tggaccggtt cgcaggggtc tccatcgatc cgaagttcac caaagccgat acggacgaca
    11641 tcgtcgcggc gatacggaag gtttatcccg cgctttcggc cgcgtgatac gagcttcgga
    11701 ccaggggtcc ggattcgacg tggcggaacc ccatttcgag accgtctatg cgatattggt
    11761 cgaattcctc gcgtggaaca tagcggatga ccggaaggtg cttaggcgac gggcgaagat
    11821 actgtcccag cgtgaggatg tcgacatcgt gggcgcggag atcgcgcatg acttgcaaaa
    11881 cctcatcgcg cgtctctccc agcccgagca tcagccccga cttggtgggg atttccgggc
    11941 ggcatttctt cgcatacgcc agcatctcga gcgagcgctc atatcttgcg cccagccgga
    12001 cctgccggta caagcgaggt accgtctcgg tgttgtgatt cagcacgtcc ggcgcggcac
    12061 tcattacgag ttccatggcg gcttgcgaac cctggaaatc cggaatcagc acttccactc
    12121 tgcaaccggc gacacggtga cgaatcgcct cgatcgtgag cttgaataac tccgccccgc
    12181 cgtccttccg gtcgtcgcga ttcacgctgg tgactacggc gtaacgcaag cccagcgcgg
    12241 cgacagcctc ggccacgcgc cggggctcgt cgtagtccac cggtaacggc cctccctttt
    12301 gcaccgcaca gaaaccgcag cggcgcgtgc acacgtttcc aagaatcatg aacgttgcgg
    12361 tgcggcggtt ccagcactcg ccgacattgg gacaggccgc gctctcgcac actgtatgca
    12421 aacgcaactc gtccaccagc gttttcagct cgcggtaatt ttcgccaccg ggagcgggag
    12481 cgcgcagcca tttaggccgt tgtggagttg tttccagagt aaccagcact acgcttcatt
    12541 ttagcgcgcg aagccgttct tctatgcctg ctacgaacgt tgcgtccttc acgagcggga
    12601 tattcgcggt cacgctggca agagctccga tgagcgtcgc ctgcgccagc gcctttgccg
    12661 tttccatttc cggtgcgaac ctgggcggcg cggcggcttc gagcttttcg atttccagtt
    12721 ggaggccgcg cgtgcactcc atcacctcca ggggtacgag cgtcgctttc tgcaatgctt
    12781 cctgaaggtg cttctcccgt tcggcgcccg gcctttttcg cgccgacacc accgcttcat
    12841 aagcggcgct gtcccgtgtg actgcttcct gaagcagtcg ccgtgcgtct tcgaagacgg
    12901 catcgggttg gtcggccatt cgcgccacca tggcgcccag agcggcggcc atcgccgctg
    12961 ccgcggcgga tgcgcttcct ccggcaggcg cgggcgtggg agcggccagc gcgtcgagaa
    13021 aatcgtgcag gcccgtttcg gaagccgcca catccgccaa ccggttctcc aggatcgtat
    13081 gggtgtggaa gctttcgaaa cgcaggtagt gctcggccgt catttcgatg gctttcttgg
    13141 gcacaagccc aatcaactcg ctgcccacga tgggtacacc aaaccggctg gcttcgaggc
    13201 tcaccatttc gaacacgcgg tgaatgggcg tttgttcgaa gtccgtcaaa ttcatggaga
    13261 cctgaacaat gccgcgagat tccagcgcaa ggcccagcgc tttcacatac cggagcccgc
    13321 ccgatgaaaa gcgcacggcg cgtgctatgt gtttggcaat ttcgagatcg gacgtaccga
    13381 ggttgatgtt gaaggcaatc agaaatttcc gggctcccac tacggtagcg ccggcggtcg
    13441 ggtgcagcgc cggtccacct acatccggta cgcgctccgc atccgtcatc accgccgagg
    13501 agagcccctc gaaatttccg cgccgtatgt tctccaggtt ggtgttccgc ggtttcgtgg
    13561 ctgccgcctc gtagaaatac acgggcacgt tccatcgggc ccagatctgc tcgcccgtcc
    13621 agcgcgcgat tcgaacgcaa tcgtcgaggg taacgccttc gacgggaacg aaaggaacca
    13681 cgtcggccgc accgattcgc gggtgcacgc cattgtgcgc ggtgagatcg attaactcgg
    13741 ctgctttgcc aacggcgcga acggcagctt cgccaacggc ttcgggcgcg cccacgaatg
    13801 taatcacgga acggttgtgg tcagggtcca actcacggct caacacaaac gttcccggca
    13861 ccgaaagaat agcgtctgcg acagcctcca caacgtcacg attgcggcct tccgagaaat
    13921 tcggaacgca ctccactaac tgtctggcca aaaatgcctc caacggggcc gctccctcac
    13981 ggtcgcggct cagaaaccga gccgtgcgcg ttagcaagcg gtcagactac gacctcgccc
    14041 gcacggtaga tcgggacgcc cttcctaatt gttatcgcca ccaggttcgt tccgcaccaa
    14101 tacgatagtt cccggtaatc ggaagcgtcc aggattagca ggtccgcctg cttcccgtat
    14161 tcaatggagc ctacctggcc ggctcgccga agggcatgag caccgtttac ggtggcggcc
    14221 gaaacgcact cggcgggtgt catttgtaat tgcgcgcagg cgaaggctat gacggcttgc
    14281 atattcgagg tgcaagcgat cgaggaatcg aaccccgtgg ccagcgctac ggctgcaccg
    14341 tggtcgatca gttcccgggc aggcggcagg tgcccggttt ttccaagtag gatgccgggc
    14401 gtcagggccg caaccgtgtt cgaacgggcg agcagagcgg cgtccaccgt gttcgcgtag
    14461 tcgagatggt ccacgctgac cgcgccggct tcgacagcaa cgcaaacgcc ctcatcatgc
    14521 gttttagttt gagcgtggac ctttagcaga aacccttgct gcttcgcgca ggccagatat
    14581 ttgcggcatt gttccggcga aaagccgtcc ggcccgcagt gaatatcgac gaaacgtgcg
    14641 agtttgcggg atcgtatgac agggagcatc tctgcgcaaa tccaggcgag atactcgcag
    14701 gagccgctcg attccggtgg gacgcgatgt gcgccgaagt acgtcggctc gatatcggca
    14761 atcgtcccat tgagagagtt gaaggtgcgc aggcatttca actccccggc ctgatccagc
    14821 aagtaacctg atttagcttc aatggtggtg gttccgtgcc ggacaaaggc ccggattgct
    14881 ttggctgccc aaaactctcg ccggcgggcg gagttgcgcc ggacctcgcg catcgtagca
    14941 tcaaatcctc cgcccgcttt tcgaatcgcg tcctcgccgg cgccggctaa ccgcatctca
    15001 tgatcggtca tacgcggggt gccgctgatg aggtgcgtgt ggctgtccac aaacccgggc
    15061 attaccacct gtccggacgc atcgatcacg tgggccttcc gcgactccgc cagattttcc
    15121 acgcgccggc tggtgccaac ttccacaacg accccattgc tgatcagaac agagccgtcc
    15181 tcgataattc caaggtcccg gagcgcggag ccgcggcgcg gcccacttgg gccccgaagc
    15241 gtcagcagtt gccttgcccc acgtatcaga agatacttgg tttgcgtcac actgcctcgc
    15301 tgtccaatac cgtgccaagg ctctattgcg ctcgtattct agcgcaaccg gccccgcgtg
    15361 tagccgctca tgccgtaagc tggtggacag gagattccag accgacttga ttcaatcccc
    15421 gccggcggga cggtgggact ttctcgcaaa gccgcgccgg tttttgagcc ccatctttcg
    15481 atactggatg gaaaccgaag tccatgttta cgggctttcg atcggcgcca gtgtgctgct
    15541 gtctttcttt ccgtttctga tcgtcatggt gtcgctgtgc cggtacgcgc ttcaatggcc
    15601 cgccgccgaa ggcgccatct atctcgcgct ggaagactat tttccgggcg aaatgggcaa
    15661 cttcatccgc cggaacctgc gggctacggt tgcctcgagg gggccgtttc aggtgatcag
    15721 cgtactgctg ctcctgttta ccgcgaatgg cattttcgag ccgctggaag tcgcgttgaa
    15781 tcgtgtctgg gggtgcgcga agaaccggag ctacgtccgg aaccaactgg tgagcctggg
    15841 attgatcttc gcctgtggaa ccctggcgat gatttccatg atcttaacgg cgatgaacca
    15901 gacatttctc acccgtctcg tgggaacgaa caccgttacc gcggcgtttc tgggtggcgt
    15961 gcttttcaaa atggccgcca tcccgatctc cattctgatt cttctgctcg tttactggct
    16021 gctccccaat tgcaaggttc cca
//
LOCUS       DASTMK010000537         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_540764, whole genome shotgun sequence.
ACCESSION   DASTMK010000537 DASTMK010000000
VERSION     DASTMK010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540764"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFQ79_22275"
     CDS             complement(<1..718)
                     /locus_tag="VFQ79_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142465.1"
                     /translation="MTAKAAIRKQSILNPAALEQLFSVLESQEFRIVGPTIRNGTIQY
                     DYIKHCEELPSGWTSEQERASCRLKRREDDAVFGYVPGADSLKRFLHPPELRLFRAER
                     ENGVFRILDNPAPAPKLAFIGVRSCELRALSILDRVLAEDRYPDAAYVAARSRIFIVA
                     VNCSDPAPTCFCASMGSGPEAAGGFDLLLTELVSDNQHVFVAEAGTSEGSGVLAALNP
                     GEVSKEKKAALRETMDRSKAA"
     gene            complement(883..1146)
                     /locus_tag="VFQ79_22280"
     CDS             complement(883..1146)
                     /locus_tag="VFQ79_22280"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEU0142466.1"
                     /translation="MHESSLISGLIDKVVALAHDNNASTVKAVEVSIGALAGISPEYL
                     MEQFRAAAAGTIVGKAELRIDVSTDEHSPRVHELLLITVELER"
     gene            complement(1128..1313)
                     /locus_tag="VFQ79_22285"
     CDS             complement(1128..1313)
                     /locus_tag="VFQ79_22285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142467.1"
                     /translation="MAATTATPTQFVLTPKPSDLLLTKPSSTNTYNAVDHKNCEVMPL
                     GVVIDGVDEVTACTNHP"
     gene            complement(1516..2916)
                     /locus_tag="VFQ79_22290"
     CDS             complement(1516..2916)
                     /locus_tag="VFQ79_22290"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU0142468.1"
                     /translation="MSIHFDHTNMLAAMVGEKNGLTAREIDSARGAAQEALAGFRKSS
                     EQGLYGFPHLPFQKDTIQEIRRFAEEVRGSYDTVCVVGIGGSALGAWALDNGMRGPHP
                     VQGEYSAEFPRLVILDNVDPSFIAAALDSMNPKKTLVVVIAKSGSTAETVATFLVVRD
                     WMVRKLGKKASRRIVAVTSAGKGDLKTLATAENYRTFHIPDNVGGRFSVLSPVGLVPA
                     ALIGIDIRKLSKGAAAMTHSCWQEDLAENQALRAALLHYLLLTRKGKTIQVAFPYSNR
                     LWGLAFWFRQLWAESLGKARNRKGKAIYAGQTPVAALGTTDQHSQVQLYMEGPNDKVF
                     SFWAVGRFPDAGKIPKNRTGLEAFDSLAGQSLEKLIQAERVSTAAALTGNGRPNCTFT
                     LDRIDEEHLGAFLQLMEFETAFAGELLDINAFDQEGVELGKKFTYGLMGRSGFEEYRG
                     RFEEYEKKRQAAGADR"
     gene            complement(3003..>3485)
                     /locus_tag="VFQ79_22295"
     CDS             complement(3003..>3485)
                     /locus_tag="VFQ79_22295"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU0142469.1"
                     /translation="DFPAPAASSSRLLRTSVEAEVCCDAPVASLVHRLLAVALDLSIV
                     VIASGVVTSMYYFAGGELRFTKQTVPVLFAVPALLYFFYSAICCIARKETPGMNWTGL
                     TLLNFDGHRPTVKQRWARLGGTCLAILAGGLGILWALGDEESLAWQDHISGTFPSYRL
                     "
BASE COUNT          729 a         1051 c          964 g          741 t
ORIGIN      
        1 aggccgcttt ggagcggtcc atcgtttcac gcagtgccgc ttttttttcc ttggaaacct
       61 ccccgggatt cagggccgca agcacgccgg aaccttcaga agtgcccgct tcggcaacga
      121 aaacgtgctg gttgtccgag acaagctcgg tcagcaacag gtcgaacccg ccggccgctt
      181 ccggaccact gcccattgac gcacaaaaac aggtgggcgc aggatcgctg caattcaccg
      241 ccacgatgaa aatccggctg cgcgccgcga catatgccgc atcgggatac cggtcttccg
      301 ccaggacgcg gtcgaggatg ctgagcgcac ggagttcgca gcttcgcaca ccaataaagg
      361 caagcttggg cgcaggcgcc ggattgtcga gaatccggaa tacgccgttc tcccgctcag
      421 ctctgaacag acgcaactcc ggcgggtgca gaaagcgctt caagctgtcc gcaccaggca
      481 catacccgaa taccgcatca tcctcccgcc ttttcaggcg gcaggatgcg cgttcctgtt
      541 cgctggtcca tccggagggc agctcttcgc aatgttttat gtagtcgtac tgaatggttc
      601 cattcctgat ggtgggaccg acgattcgaa attcctggga ttcaaggaca gagaagagct
      661 gttcgagggc cgccggattc agaatagact gcttccgtat tgcagctttg gccgtcatga
      721 taaccgccgt gccacagagt cagcacttgg cgtaccacag ttaagcaact aagaacactg
      781 aagtttcctc acccgcgaaa cgtaatgggt gaaactacgc gattttaaga tctttttatc
      841 gtcaattagc gcactcactt ccttcgtacc gcgcgcaatg actcaacgct ctaactccac
      901 cgtgatcagc aacaattcgt gtacacgagg agaatgctca tcggttgaga cgtcaattcg
      961 aagttccgcc ttcccgacaa tcgttccggc cgctgccgcg cggaactgtt ccatcaagta
     1021 ttcgggactg ataccagcca aagcgccaat cgaaacttcc acggccttca ccgtcgacgc
     1081 attgttgtcg tgagccagtg caaccacctt gtcgatcaag ccggagatca gggatgattc
     1141 gtgcacgccg tgacctcgtc tactccgtcg atgaccaccc ctaacggcat cacttcgcaa
     1201 ttcttgtggt cgacggcatt gtaggtgttc gtactggatg gttttgttag tagtagatcc
     1261 gaaggctttg gcgtcagcac gaactgcgtc ggtgttgcag ttgtggctgc catggcaacc
     1321 ttcgatcaca ggaaagcacg tggcgtgcca gaatgaattg accactccct cacggtcatg
     1381 gctcagagaa atttgttatt accgcagcac ttgcgatcaa gccgcgcgtg agcgagcggc
     1441 cggttaaccc atgtaacaga gagaatgggg tgaaataacg cgatttatag atccggatct
     1501 cgttaattga ctcacctatc gatccgcccc ggcggcctgg cgcttttttt catattcttc
     1561 gaagcgtccg cggtattctt cgaatcctga acgccccatc aacccatagg tgaacttctt
     1621 tccgagctcg actccctcct gatcgaaggc gttgatgtcg agtaattcgc ccgcgaacgc
     1681 ggtctcaaat tccatgagtt gcaggaacgc accaagatgc tcctcgtcaa tccggtcgag
     1741 ggtgaaggtg cagttcgggc gcccatttcc agtgagagcc gccgccgtgg agacgcgctc
     1801 ggcctgaatc aatttctcca ggctctgtcc ggccagagaa tcgaaagctt ccaggcctgt
     1861 gcgatttttc gggatcttgc ccgcgtccgg gaacctgccg acggcccaga aggagaagac
     1921 tttatcgttc gggccttcca tatacaactg aacctgcgaa tgctggtctg tggttccgag
     1981 cgctgccacg ggcgtctgac cggcgtagat ggccttgcct ttgcggtttc tggctttgcc
     2041 gagcgattcc gcccatagct gccggaacca gaaagccaat ccccatagcc ggtttgagta
     2101 ggggaaggcc acctgaatcg ttttgccctt tcgggtcagg aggaggtagt ggagcagtgc
     2161 cgcccgtaag gcctgatttt cggccaggtc ctcctgccag caggagtgcg tcatggcggc
     2221 agcgcctttc gacagctttc gaatatcgat tccgatcagc gccgccggaa ccagaccgac
     2281 gggcgacagc acgctgaagc gcccgccgac gttatcgggg atatgaaatg tccggtagtt
     2341 ctctgcggtc gcgagcgttt tcaggtcacc tttgccggcc gaggtgaccg ccacgatgcg
     2401 ccggcttgcc ttcttgccta atttccgcac catccaatcg cggaccacca gaaacgtggc
     2461 caccgtttcg gcggtactac ccgatttcgc gatgaccacc accagggtct ttttgggatt
     2521 catcgaatcg agagccgccg cgataaagga cggatcgacg ttgtcgagaa tcacgaggcg
     2581 tggaaattcc gcggagtact caccctgcac cgggtgcgga ccgcgcatgc cgttgtcgag
     2641 cgcccatgcc cccagcgcgc tacccccgat cccgaccacg cacacggtat cgtatgagcc
     2701 ccgcacttct tccgcaaacc gccgtatctc ttgaatcgtg tccttttgaa acggcaggtg
     2761 aggaaatccg taaaggccct gttcgctcga cttccggaaa ccggccagcg cctcctgagc
     2821 cgccccgcgg gcgctgtcga tctcccgggc cgtgaggcca ttcttttcgc ccaccatagc
     2881 ggcgagcata ttcgtgtggt cgaaatgaat actcatgatg gattttactt gcctgtgttt
     2941 ccccgtttag aagcggagcc gcgcgcgtga gcaagcggtt ttcgtccttt ttcggcagcc
     3001 tgctacagcc ggtagctggg aaacgtgccg gaaatgtgat cctgccaggc caggctctct
     3061 tcatcgccca gagcccacag gatgcccaag ccccctgcca gtatggcaag gcaggtaccg
     3121 cccagtcgag cccagcgttg ctttaccgtg ggccggtgcc cgtcgaagtt cagtagtgtg
     3181 agacccgtcc agttcattcc cggagtctct tttcgcgcaa tgcagcagat ggcgctgtag
     3241 aagaaataga gcagcgccgg aacggcaaac agcacgggga cggtctgctt cgtaaagcgg
     3301 agttctcccc cagcgaagta gtacatcgac gtcacgacac cgctggcaat taccacgatg
     3361 ctgagatcga gtgccaccgc aagcaacctg tgcaccagcg aagccaccgg cgcatcgcaa
     3421 catacttcgg cttcgacgga ggtccgcaaa agcctgctcg aggaagcagc cggagcggga
     3481 aaatc
//
LOCUS       DASTMK010000538         5974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_251587, whole genome shotgun sequence.
ACCESSION   DASTMK010000538 DASTMK010000000
VERSION     DASTMK010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251587"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VFQ79_22300"
     CDS             complement(<1..1448)
                     /locus_tag="VFQ79_22300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0142470.1"
                     /translation="MRTGFYSPLPPANTGVADYSATLIEAMRGFGRVDVNPAEADVRL
                     YHLGNNPLHREIYRRALEQPRVIVLHDAVLHHFLLGSLSKEQYIDEFVYNYGEWMRDA
                     AQDLWRGRARSAATREYFRYPMLKRIVEASTGVVVHNPGAAAMVRLHCPRARVFEIPH
                     LYRAAAAAIGVGRLKDRLGLGPHHFVFGVFGYLRESKRLMPVLRAFRLVRRAQPNAAL
                     LVAGSFASADLERAAEPLLASPGVIYTGTTPQQEFDALITRVNVCINLRHPAAGETSG
                     ITIRAMGAGKAVILTDTPETSRFPENMCLRIAADPDEERTLAALMLWLAQNPAAAREI
                     GCRAASYIEREHNLERVARLYWEALGYNLAMAKETQPMSVDAIQAKSGRIVRLDSALD
                     AIVSESAAIEKLAGGFSFTEGPVWIPEGYLLFSDIPENTIYKWSPDTGIEVFRKPSGC
                     DRIGVAPGVFRGSNGLTLDAQGRLTICEHGNRR"
     gene            complement(1445..2101)
                     /locus_tag="VFQ79_22305"
     CDS             complement(1445..2101)
                     /locus_tag="VFQ79_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU0142471.1"
                     /translation="MKDRLEAVEERILRAVGRSGRKREEITLIAVTKVFPAQAIVDAY
                     ALGLRHFGENYVQEFDAKWDAVKHLDDARFHLIGHLQSNKARRAAEIFHTIQTVDRSK
                     LARRLDEAGRTLEVMLEVKLSEEETKTGAAPEELPALIETVRACSNLKLTGLMTMPPW
                     SDDPEQARPYFRRLREIAARNSIDGLSMGMSHDFEAAIEEGATHIRVGTALFGERKRQ
                     "
     gene            2126..3643
                     /locus_tag="VFQ79_22310"
     CDS             2126..3643
                     /locus_tag="VFQ79_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142472.1"
                     /translation="MSMDSGEAPRREAQEACQAIERFLKASSEPFLQEPGEDNLPLRG
                     DNHVIEWQGSRLVLQAWDQKRNLVRRITAVGAERPGRLDVVTEKFGKRPGTLTFFDAG
                     RPANQFLGKRAARLAFREHFRRFLRRRFTDWRLCELTTEQDLEHSLSPSYARAFLKKG
                     QQGIAAIGCSAESNAEGLLTFGLIWLDYLRQRERKHTIEGLLLYLPAGIEQNTCLRLR
                     WLNPRSATFQVFVFSEQGVDDCVDLRDYGNTDTHMEPCLSADALCPPDLAELARLISG
                     IHDVTTLASKNGELSFRIRGLEFARIAGGCLQFGIETKQIGTASNLREIERLVSEVAR
                     LRSGQACDRSNPFYSRDPEAWLESQVRTHMEELEPSLLPNPVYGQVPAFAAGNRGVID
                     MLGVDRQGRLAVLELKAEQDIHLPIQALDYWIRVKWHLDRGEFSGQGYFPGIRLSAEA
                     PRMLLIAPALQFHPATESILQYFSREIEIQRLGLGPDWRGTPKVVFRAFGAQRPG"
     gene            3666..4550
                     /locus_tag="VFQ79_22315"
     CDS             3666..4550
                     /locus_tag="VFQ79_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142473.1"
                     /translation="MFKQIRQAITSLNPEEVRATAERPVHIALIASTSQGYAEMEDFL
                     LPGNISHDKRMEAMNTLHRVGDIGTPHNFDLEIYEEGLPHSETGFTFNPRDPGQTVSE
                     ILAVRDDLGLALARYYPAFRKAVSHHLVKTVSKENALFSLATALPNVLPSAVALPWAL
                     GEFASDTAFLTTNQIRMAFLMAAASDRAVGYRNQKSEIASIIASAFGWRALARELAGK
                     IPFGGGLLPKAAIAYAGTYVVGSSIERYYRIGYGYSRSERKAMFGDAYEKGRRVAGTL
                     LENIKRREGTAIEPGTSS"
     gene            4613..5452
                     /locus_tag="VFQ79_22320"
     CDS             4613..5452
                     /locus_tag="VFQ79_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142474.1"
                     /translation="MIPRLSAVFLLVFSIGLADTLTLRSGRTVEGTFLGGDSRQIRLA
                     VGDKIESFDIRDVSGIQFGAPQAAPAPAAAPTAATVSPEPVSSTAPRKAEILRPEPAA
                     TTTASSASSTAREIPAETAIVIRMIDDVDSERDSVGKTFRASLDEPVMVNGEVVAPRG
                     NDVTVKLVSDEQSGRLTGRTELTLDLVSLQIGDKTVDIDTQAVTQASESRTGQSAKVV
                     GGTAALGAIIGAIAGGGRGAAIGAVSGAGAGTAVQVLTKGQKVRIPSETRLQFTLQQP
                     VRL"
     gene            5483..>5974
                     /locus_tag="VFQ79_22325"
     CDS             5483..>5974
                     /locus_tag="VFQ79_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0142475.1"
                     /translation="MQTAIVTGASRGIGRGIAKELSRTHRVIATFKGRRDAAESLRQE
                     TGVEIFQCDIASAASRDALIAFARERFGTLDLLVNNAGIAPRERRDIVEASEEIFDEV
                     LSTNLKGPYFLTQQAARWMLEKGAGRITFVTSISSYTASVNRGEYCISKAGLSMAVAL
                     WAQR"
BASE COUNT         1171 a         1811 c         1807 g         1185 t
ORIGIN      
        1 cgccggtttc cgtgttcgca aatcgtcaac cggccttggg catcgagcgt caatccgttg
       61 gatccccgga acacaccggg cgcaacaccg atccggtcgc atccgctggg tttgcgaaat
      121 acctcgatgc ccgtatcggg actccactta taaatcgtgt tctccgggat atcgctgaac
      181 agcaggtagc cctcgggaat ccagaccgga ccttcggtga acgagaatcc gccggccagt
      241 ttctcaatcg ccgccgattc agagacaatc gcatccagtg cggaatcgag gcggacaatc
      301 cttccgcttt tcgcctgtat cgcatcaacg ctcatcggtt gggtctcctt tgccattgcc
      361 agattgtatc ccagcgcctc ccaatagaga cgcgcgacac gctccaaatt gtgctcccgt
      421 tcaatgtaag acgccgcacg gcatccaatt tcccgcgccg cggcggggtt ttgtgccagc
      481 caaagcataa gcgccgccag ggtgcgctct tcgtccgggt ccgccgcgat gcgcaggcac
      541 atgttttccg gaaatcgcga cgtctccgga gtgtccgtga gaataaccgc ctttcccgct
      601 cccatggcgc ggatggtgat tcccgatgtc tcgcctgccg ccgggtgccg cagattgatg
      661 cacacgttca cacgcgtgat cagggcgtcg aattcctgtt gaggtgtggt gccggtgtat
      721 atcactccgg gcgaggccag caaaggctcg gccgctcgtt cgagatccgc cgaggcgaac
      781 gatcccgcaa ccagaagggc cgcattcggc tgtgcccggc gcaccaggcg aaaggcgcga
      841 agcaccggca tcaggcgctt cgattcccgc agatagccaa atacgccaaa cacaaagtga
      901 tgcggcccga gccccagacg atctttcaac cggcccaccc cgatcgcggc ggctgcagcg
      961 cgatagagat gcggaatctc gaaaacgcgc gccctggggc agtgcaaacg taccatcgcg
     1021 gctgcgcccg ggttgtgcac aacaacgccg gtagaagctt cgacgatccg cttcagcatc
     1081 gggtaccgga aatactccct ggttgcggca gaccgggcgc gcccgcgcca caggtcttgc
     1141 gcggcatcgc gcatccattc accgtagttg tacacgaatt cgtcgatgta ttgttccttg
     1201 ctcagagatc cgagaaggaa atggtgcagc acggcgtcgt gcagcacgat gacacgcggc
     1261 tgttcgagcg ccctccggta aatttcccgg tgcagcgggt tgttgccgag atgatagagg
     1321 cgcacgtccg cctccgcggg gttcacatcc acgcggccga acccgcgcat ggcttcgatc
     1381 agcgtggcgg agtagtccgc cacgccggta ttcgccggtg gaagaggcga gtagaatccg
     1441 gttctcactg ccgctttcgc tcgccgaaaa gcgccgtacc tacgcggatg tgcgtggctc
     1501 cctcctcgat ggccgcctcg aaatcgtggg acatgcccat cgacagcccg tcgatgctgt
     1561 ttcgtgcggc aatctcgcgc aaacggcgaa agtacgggcg cgcctgctcc ggatcgtcag
     1621 accagggggg catagtcatg aggcccgtta gtttcaggtt cgaacaggcg cggactgttt
     1681 caattaaagc cggtagttcc tccggggccg cacccgtttt cgtctcttcc tccgacagct
     1741 tcacttcgag catcacttcc agcgtgcgtc ccgcttcatc caggcgccgc gccagcttgg
     1801 aacggtccac tgtttggatc gtgtgaaaga tctccgccgc ccgccgcgcc ttattcgatt
     1861 gcaggtgccc gatgagatga aaccgcgcgt cgtccaaatg cttcacagca tcccatttgg
     1921 catcaaattc ctgcacgtaa ttctcaccga aatgacgcaa ccccagcgcg tacgcatcca
     1981 caatggcctg tgccggaaac actttggtaa cggcaatcaa agtgatctcc tcgcgtttgc
     2041 gccccgagcg tccaacggcg cggaggattc gttcctccac agcttccaac ctgtctttca
     2101 agtgggtcaa atataataat gacggatgtc catggactcc ggcgaagccc cccggcgcga
     2161 agctcaggag gcgtgccagg cgattgagcg ttttctcaaa gccagcagtg aacccttcct
     2221 gcaagaaccc ggagaagata acctgccgct acggggagat aaccacgtca ttgaatggca
     2281 gggaagccgt cttgtgttgc aggcgtggga ccagaagcga aacctggtac ggcgcatcac
     2341 agccgtcggt gcggagcggc ccggccgcct ggatgtcgtc acggagaagt tcgggaaacg
     2401 gccgggaacg ctgacctttt tcgatgccgg acgcccggcg aatcaattcc ttggaaagag
     2461 ggccgcgcgg cttgcctttc gcgagcattt ccggcgtttc ctccggcggc ggttcacgga
     2521 ttggagactc tgcgagctta cgacggagca ggatcttgaa cacagcctgt cgcccagcta
     2581 cgctcgcgct tttctgaaaa agggccagca gggcatagcg gccatcggct gctccgcgga
     2641 gtccaacgcc gaaggactgc tgacgtttgg actgatttgg ctcgactatc tccggcagcg
     2701 cgagcgaaaa cacaccatcg agggactgct gctgtacctg cctgctggca tcgagcagaa
     2761 cacctgcctc cgcctgcgat ggttgaatcc gcgatcggcc accttccagg tattcgtatt
     2821 ttccgaacaa ggagtagacg actgcgtgga cctgcgcgac tacgggaata cggacaccca
     2881 catggagccg tgcctgtccg cggatgcgct gtgtcctccg gatctggcag agctggctcg
     2941 gcttatttca ggaattcacg acgtcacaac gctggcctcc aagaacgggg agttgagctt
     3001 ccggattcgt gggcttgagt tcgcccgcat tgccggcggg tgccttcaat tcggcatcga
     3061 aaccaagcag attgggacgg cgtctaattt gcgggagatc gaacgtttgg tttctgaagt
     3121 cgcacgcttg cgcagcggac aggcgtgcga tcgttcaaat cccttctatt cgcgcgatcc
     3181 ggaggcctgg ctggagtcac aggtgcgaac gcacatggag gagttggaac cttctctcct
     3241 gcccaatcct gtgtacggcc aggtcccggc atttgccgcc ggaaaccgtg gtgtcataga
     3301 catgctgggt gtcgaccggc agggcaggct ggcggtactg gaactcaagg cggagcagga
     3361 cattcacctg ccgatacagg ccctcgacta ttggattaga gtgaaatggc acctggaccg
     3421 cggcgagttc tccgggcagg gatattttcc gggaatccgt ctctccgcgg aggcgccaag
     3481 gatgctgctg attgcgccgg cgcttcagtt tcacccggca acggagtcga tcctgcaata
     3541 cttctcccgc gagatagaga ttcagcgcct gggcctcggc ccggactggc gcggcacgcc
     3601 aaaggtggta ttccgcgcgt ttggcgcaca gcgcccgggg taaaattcac agcatggcgg
     3661 agacgatgtt taaacagatc cggcaggcaa tcaccagcct gaatccagaa gaggtgcgtg
     3721 caactgccga aaggccggtt cacatcgccc tcatcgcatc cacaagccag ggatatgcgg
     3781 aaatggaaga ttttctgctg cccggaaata tctctcacga taaacggatg gaagcgatga
     3841 acacgctcca tcgcgtgggt gatatcggaa cgccgcacaa tttcgatctc gaaatctatg
     3901 aggaaggact gccgcattcc gaaaccggtt ttaccttcaa cccgcgcgat ccggggcaga
     3961 ccgtcagcga gattcttgcc gtgcgcgacg acctgggttt ggctttggct cgctactatc
     4021 ccgctttccg caaagcggtg tcccatcatc ttgtcaagac agtttcgaaa gagaatgcgc
     4081 ttttttcgct tgcgacggcg ctgcccaatg ttttgccatc cgcggtggct ttgccgtggg
     4141 cccttggcga gttcgcatcc gacacggctt ttctcacaac caaccagatc cgtatggcct
     4201 tcctgatggc cgctgccagc gatcgcgccg tgggctatcg caaccagaag tcggaaatcg
     4261 cgtcgatcat cgcgagcgca ttcggctggc gtgctctggc acgggagctt gccgggaaga
     4321 tcccgtttgg cggagggctg ctgccgaaag cggccattgc ttacgcggga acctatgttg
     4381 tcgggtcttc gatcgaacga tactaccgca tcgggtacgg atactcccga tccgagcgaa
     4441 aagccatgtt tggcgatgct tacgagaaag ggcgccgtgt cgcgggcacg ctactcgaga
     4501 atataaaacg gcgtgaagga accgccattg agcccgggac gtccagttag tctgaccggc
     4561 tgtctgagcg gttgtctata atcagattta ccggattgcc tagaggtgga ctatgatccc
     4621 gagactgagc gccgttttcc tgttggtttt ttcaattgga ctcgccgata ccttgaccct
     4681 gcgaagcggc aggacggttg agggaacttt tttgggcggg gattcccgcc agatccggct
     4741 ggccgtcggc gacaagatag agtccttcga catccgcgac gtatcgggta tccagttcgg
     4801 tgctccacag gccgcgccgg cacccgctgc cgcgcccact gctgcgacag tctcgccgga
     4861 gccggtttcg agcaccgctc cgcgcaaagc ggagattctc cgtcctgaac ctgcggccac
     4921 aaccacggca tccagcgcgt cttcgacagc ccgtgagatc ccggcggaga cggcgatcgt
     4981 catacgaatg atcgacgacg tagattccga gcgtgacagt gtaggcaaga cctttcgagc
     5041 cagcctggac gagcccgtca tggtgaatgg cgaagtggtt gctcctcgcg gcaacgatgt
     5101 gacggtgaaa ctggtgtccg atgagcagtc cggccgcctg accggccgca cggaactgac
     5161 cctcgatctc gtgtcgttgc agatcggcga taagaccgtc gatatcgata cgcaggcggt
     5221 cacccaggcc agtgaatcgc ggaccgggca gagcgcgaaa gtcgtcggtg gcacggcggc
     5281 gctcggagcc attatcggag cgattgcggg aggcggcagg ggtgccgcca tcggagcggt
     5341 atccggcgcc ggcgcaggca ccgccgtcca ggtcctcacg aaaggacaga aggttcgcat
     5401 cccgtcggag acacggcttc agttcactct ccaacagccc gtccgcctgt aaattcgggc
     5461 ggcggccggg ctgcacgatc tcatgcagac tgctattgtt accggggcga gccgcggcat
     5521 cggccgtggc atcgccaaag agttgtcccg cacgcaccgc gtgattgcga cgttcaaagg
     5581 ccgacgggac gcggccgagt cgcttcgcca ggaaaccggc gtcgagattt ttcaatgcga
     5641 catcgcttcc gccgcgagcc gcgacgcgtt gattgccttt gccagggagc gttttgggac
     5701 tttggacctg ctggtgaata acgcgggcat cgcgccgcgc gaaaggcgcg atattgtgga
     5761 ggccagcgag gagatcttcg acgaggtgct ctcgacgaat ctcaagggcc cgtattttct
     5821 gacccagcag gctgcgcgct ggatgctcga aaagggcgcc gggcgtatta cgttcgttac
     5881 gtccatttcc tcctatacgg cttccgtcaa tcgcggtgaa tactgcatct cgaaagcggg
     5941 gctcagcatg gccgttgcgc tatgggcgca gcgc
//
LOCUS       DASTMK010000539         8464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_474668, whole genome shotgun sequence.
ACCESSION   DASTMK010000539 DASTMK010000000
VERSION     DASTMK010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474668"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFQ79_22330"
     CDS             complement(<1..668)
                     /locus_tag="VFQ79_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0142476.1"
                     /translation="MKTVILDGYCLNPGDLSWDALRELAELEIHDRTPASQVVERAQG
                     AEAVFTNKTPLPRDVLRQLPDLRYIGVLATGYNIVDVAAAREAGITVTNIPTYGTASV
                     AQFAFALLLELCHHVGDHAAAVRAGEWSTNPDWCFWKTPLVELSGKTMGIVGFGRIGR
                     QVGRIADAMGMKVIASDEVRENTPGYDGFRWVEVDDLLATADVVSLHCPLLAATEGII
                     AKERL"
     gene            complement(689..1618)
                     /locus_tag="VFQ79_22335"
     CDS             complement(689..1618)
                     /locus_tag="VFQ79_22335"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEU0142477.1"
                     /translation="MFEKLDQLVDAARAGGPVQIAIAAGQDPDVIEAMKRAEEIGLAK
                     GVFVGHPDKIRSLAAAADWEIPDDQLIPEPDDDAAARKAIALIREGRAALLMKGKIKT
                     SSLVKAVLDKEAGLRAGRLLSQVIVFEVPGWNRLMLMTDAAINIAPTLDQKAEICRNA
                     IEVAHAIGIENPHLALLCALELVNADMPATVDAAALTMMNRRGQLTGVYVEGPIALDV
                     PLSRFAADRKKIDSPLVENTDIFVAPDIEAANILYRAILYFAKGESGGIVLGARVPLI
                     LLSRAETPETKIRSIAIGILLARSQQNKLRAAV"
     gene            complement(1618..2856)
                     /locus_tag="VFQ79_22340"
     CDS             complement(1618..2856)
                     /locus_tag="VFQ79_22340"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142478.1"
                     /translation="MESPWKTDRWFTSPWNHASEVKATLQFPDKIRIHDVTLRDGEQQ
                     TGIVFRRQEKVAIAKKLAEAGVHRIEAGMPAVSSEDEAAIKDIVALGLPSEIFSFARC
                     MPADVKMAKDCGVFGIITEIPSSEHIIRNAYGKTMDWAIKSSIETTLAAKEAGLYTSF
                     FTIDSTRSELEPYMDLIERVATEGHMDALTLADSFGGCTPEAIGMIVRKLKARFNKPI
                     EIHCHEDFGLGVANTIAALANGASVAHVTVTGTGERAGNVPLEDTVMALRCLYNVDTG
                     IKTEKFYELSKLVQELSGFQVPPNRPIVGDWLYNIESGIVSMFHRRCKSVEPLEYIPF
                     APELAGRPGVSIALGKGSGVANVEEHLERRGISLPQEQIDAIVARVKQTSIEKKALLS
                     EVEFEQIVQTVRDTVTAAAK"
     gene            2951..5173
                     /locus_tag="VFQ79_22345"
     CDS             2951..5173
                     /locus_tag="VFQ79_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142479.1"
                     /translation="MRAILILIGLSCALAAAQQNSSGWSELRSRNPPQLEFSIRLLNQ
                     RAYHEGELIRAEVRYPPPHFVPGREPPPPEQWQFAGYLLNPERGCGSPEKPCFGFVDP
                     AGGIGVGPGGSSGPGPVFLNRYLRELPPGRYRTAVLARKLVLTGRAPMSATYGYTTPA
                     QYAVSNDVEFEVIAAPPAWVKRTIEASVAKLKEPQPGSREGYEARTAAAQQLRFLDQP
                     AAWTASLDLLPVEEGTFLSGLAATREPKRVCELMQSRTAAPEQAVSLQYLSTMARLCS
                     RADLPPPPTSDDARQKYWTDWHNYQERILGGSISTLAASLPRKQAGGKTIAIEALLQR
                     IQQRRSIDPQGPAPDWIPVLRQEFIKSFPALESWRKSHPLTMYAAVLKGPELTSLAES
                     MLDAWKPGDYYEGPRSALHVLHALDSVRAEKRILAEVVKPKTWLDTPQLDLLPAAAVP
                     PMDDELIEALAAAQREGGWNLPLRMAALAKYATPRALSRIQAIYESQQVSCQPELMAY
                     FLRAEPAYADRVFRNHQWDMQAAPPLCILQYFERTPRLAMHTVLERYMAAYLMHRDVP
                     VKKMAAYSLGLYGSKDALFPLWEAFRYFHEYWKGKKTELEQNQEGVFLEVELRNAIAR
                     GRGWLATETDLRTIESLCISERCIYDTRQDLQNVQEPFRIEITGSGFGGLQTRIAQYY
                     GLTGMEQVKDKLAQFPPGTRFVLAAHGSDIDNASAEIRKYAAGRRLVIVLGRPDMLSQ
                     "
     gene            5170..5973
                     /locus_tag="VFQ79_22350"
     CDS             5170..5973
                     /locus_tag="VFQ79_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142480.1"
                     /translation="MSAGEQTDSIPPAWQLLERAAAKNLFDYVQAFRLATTDVLAAAT
                     DCAGGIAAYTGAGSPLTTIKGAGPDLSDDALDQAEAFFRKCNSPCVVFELAPWITPAA
                     VERLTRRQYQVRAVEHVMLLDAPFKIPEPQSGIRILNAAEWADLVFRGFEAADTPVWH
                     HLAEASALLPEAANLGVWGTSADACIACAQMVPAAGVAIFSCDATLPTARGKGAQTAL
                     IRERLRLASAIGATCAAAEVAPDGISERNYLRCGFRLAYSRKHYVRNLG"
     gene            5980..8079
                     /locus_tag="VFQ79_22355"
     CDS             5980..8079
                     /locus_tag="VFQ79_22355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142481.1"
                     /translation="MSLFHRSVVVAAITVTAAAAAVRVGTFPSEVRTFYTTGDGLPSN
                     DVLAVSIHGGKLFARTAAGGAVFGNGKWIRSEYPAEKKPDLEGLPGGVSIRQTASHAD
                     GRVAVAVEEGLFLREPSGKWSDLYPHEGSRSWHPKDVRGVAFDVSGRFWFASPQGVGR
                     FDGTWKLYTGHDGLPYNDFTTMATGEKGVVWFGTGRGAIRFDGSVWEYRQGLRWLPSD
                     EVRSIAVEEDGDAWFATPAGVGLIERRSTTLSQKARFFEDDIDKRHRRTPYGYVDSVS
                     LERPDDLSKWTQHDSDNDGLWTSMYGAGECFAYAATRDPLAKKRADAAFRALRFLGEV
                     TRGGSHPAPPGFVARTILPTSGPNPNTVYTPERDRERQATRDALWKVIVPRWPTSADG
                     KWYWKTDTSSDELDGHYFFYAVYYDLVAANEDERRQVRDHVAGLTDHLIRHGYNVVDH
                     DGKPTRWGVFSPEKLNHDPLWFEERALNSLSILSYLRTAEHITGNPKYGEAARELMTK
                     HAYAMNVMIAKTNAGPGSGNQSDDEMAFMNFYCLLKYEKDPKLRSAFAFAFSRRWMME
                     RYELNPLFNFIYAAVASGIEYENAFGRGDLSPAGDWLAESIDTLKRYPINRVSWGLSN
                     SHRTDIVLLPLYAREEGQRGSRRDGRVLPIDERFVDKWNHDPWALDYHGNGRRLADGA
                     SFLLPYYMGLYHGFIAD"
     gene            complement(8066..>8464)
                     /locus_tag="VFQ79_22360"
     CDS             complement(8066..>8464)
                     /locus_tag="VFQ79_22360"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU0142482.1"
                     /translation="ESGLKGLSGISGDMREIMAAVEVGNRRAGLAFDAYIQRLCREIG
                     GMVASLQGMDALAFTAGVGENSAPLREAVCKRFRYLGVEMDAAHNGDSPVDQDIATPQ
                     SRVRVLVIRTDEEWQIARECVRLLPSLNRR"
BASE COUNT         1640 a         2575 c         2556 g         1693 t
ORIGIN      
        1 agccgctcct tcgcgatgat tccctcggtg gcggcgagaa gcgggcagtg caggctaacg
       61 acgtcggcgg tggcgagcag gtcgtctacc tcgacccagc gaaacccatc gtagccggga
      121 gtattttcgc gaacttcgtc cgaggcaatc accttcattc ccattgcgtc cgcaatgcgg
      181 cccacctggc ggccaatccg cccgaagcct acgatgccca ttgttttccc ggacagttcc
      241 accagcggtg tcttccagaa gcaccagtcc gggtttgtac tccactcgcc cgcacggacc
      301 gccgccgcgt ggtcgcccac atgatggcag agttcgagca gcagcgcgaa agcgaactgg
      361 gccaccgatg ccgttccgta agtagggatg ttcgtcaccg tgataccggc ttcccgggcg
      421 gcggccacat ccacgatgtt gtacccggtc gcgagaacgc cgatatagcg cagatcaggc
      481 aattgccgga gtacgtcgcg gggaagcgga gtcttgttgg tgaagaccgc ttcggcgcct
      541 tgagcgcgct ccaccacctg gcttgccggc gtgcggtcgt gaatctccag ttcggcgagt
      601 tcgcgcagcg catcccagga gaggtctccc gggttcagac aatatccatc cagaatgacg
      661 gtcttcatgg gtagcagggc acgcgagctc agaccgcggc tcgcaatttg ttctgttggc
      721 tacgcgccag tagaattcca atggcgatcg agcgtatctt cgtttccggc gtctccgcgc
      781 gcgacagcag aatcagaggg actcgcgctc caagcacgat tccgccggat tcgccttttg
      841 caaaatagag aatcgctcga taaagaatgt tggccgcttc aatatccgga gcaacaaaaa
      901 tatcggtgtt ctcaacgagc gggctatcga tttttttccg gtccgcggcg aaccggctga
      961 gggggacatc gagtgcgatc ggcccctcca catacacgcc cgtcaactgc ccgcgccggt
     1021 tcatcatggt cagtgccgcc gcgtccacgg tagcgggcat gtccgcgttc accagttcca
     1081 gcgcgcacaa cagcgccaga tgaggatttt cgatgccgat ggcatgcgcg acttcgatcg
     1141 cgttgcggca gatctccgct ttctgatcga gcgtaggcgc gatgttaatg gcggcatcgg
     1201 tcatcagcat caggcgattc catcccggta cctcaaacac gatcacctgg ctcaacaggc
     1261 gtccggcgcg cagtccggct tccttgtcga gcaccgcctt caccagggac gaggtcttga
     1321 tcttgccttt catgaggagg gccgcccggc cctccctgat cagcgcgatg gctttgcgcg
     1381 cggctgcatc atcgtcgggt tccggaatca actggtcatc cggaatctcc caatccgctg
     1441 ctgcggcgag agaccggatc ttgtccggat gcccgacaaa gactccttta gcaagcccga
     1501 tctcttcggc gcgcttcatc gcctcgatga cgtcggggtc ctgtcccgcc gcaattgcaa
     1561 tctggacagg tcccccggcg cgtgcggcgt cgacaagctg atcgagtttt tcgaacatca
     1621 cttcgccgcg gcggtaaccg tgtcgcgaac cgtttgtacg atctgctcaa attcgacttc
     1681 cgacagcagc gccttcttct cgatggaggt ctgtttcacg cgggcgacaa tcgcgtcgat
     1741 ctgctcctgc ggcaggctga tcccgcgccg ctccagatgc tcctcaacgt tggccacacc
     1801 gctgcctttg ccgagagcga tgctcacgcc ggggcgtccg gcgagttccg gtgcaaacgg
     1861 aatgtattcg agaggttcca cgctcttgca gcggcgatgg aacatgctga cgatgccgga
     1921 ttcgatgttg tagagccagt ccccgacgat ggggcggttc ggaggcacct ggaagcctga
     1981 aagctcctgc accagcttcg atagctcgta gaacttctcc gtcttgatgc cggtgtccac
     2041 attgtacagg caccgcagag ccatcacggt atcttccagc ggaacgttcc cggcgcgctc
     2101 gcccgttccg gtgacggtta cgtgcgcgac cgaagcgccg ttggccagcg cggcgatggt
     2161 gttggcgacg ccaaggccga aatcttcgtg gcaatggatt tctatcggct tgttgaagcg
     2221 cgctttcagt ttgcggacga tcattccaat ggcttccggc gtacagccgc cgaaagagtc
     2281 tgccagcgtc agcgcgtcca tgtgtccttc ggtggcaacc cgctcgatca ggtccatgta
     2341 tggctcgagt tcggagcgtg tgctgtcgat ggtaaagaac gacgtgtaga gacccgcttc
     2401 cttcgcggcc agcgttgtct caatcgacga cttgatggcc caatccatcg tttttccgta
     2461 ggcgttccgg atgatgtgtt cgctggacgg aatctccgta atgattccga agacaccgca
     2521 gtccttcgcc attttcacgt cggcgggcat gcatcgtgca aaggagaaaa tctccgatgg
     2581 caagccgagg gcgacgatgt ccttgatagc agcttcgtct tcgctcgaaa cggcgggcat
     2641 tccggcttca atgcggtgta cgcctgcttc ggcgagcttt ttggcgatgg ctaccttttc
     2701 ctgacggcgg aatacgatac ctgtctgttg ctctccatcg cgcagcgtga catcgtggat
     2761 acggatttta tcggggaact gcaaagttgc cttaacttca gacgcatgat tccaggggct
     2821 ggtaaaccag cggtccgttt tccagggact ctccatataa gctcctttcg actcggggcc
     2881 agtataccat tcaggtgcct tcgggaacct tacatcccgt ggattccgtc taagctgatg
     2941 gaccggttcc atgcgcgcaa ttttgatcct gatcgggctg tcctgtgcgc tcgccgccgc
     3001 ccagcagaac tccagcggct ggagcgaact gcgatcgcga aatcctccgc aactggagtt
     3061 ttcgatccgt ttgctgaacc aacgcgccta ccacgaaggg gagttgatcc gcgcagaggt
     3121 aaggtaccct cccccgcact tcgttcccgg tagggagccg ccgccgccgg agcaatggca
     3181 gtttgcgggc tatttactca acccggagcg cggttgcgga agtcctgaga aaccgtgctt
     3241 cggcttcgta gatcccgcag gaggtatagg tgtcggccca ggaggcagta gcggccccgg
     3301 ccctgtcttt ctgaaccgat atcttcgcga actgccgccg ggacgctatc gcacggccgt
     3361 cttggcgcga aagctggtat taaccggccg ggcccccatg tcagccactt acggctacac
     3421 gacgcccgcg caatatgctg tatcgaacga cgtcgaattc gaagtgatcg cggcgccgcc
     3481 ggcctgggta aagcggacca tcgaagcgag cgttgccaag ttaaaggagc cgcagccggg
     3541 cagccgcgag ggatatgaag cccggacagc cgctgcgcaa caactccggt ttctggatca
     3601 acccgctgcg tggactgcgt cgctcgacct gttgcctgtc gaagaaggca ccttcctttc
     3661 tggtttggcg gccacgcgcg aacccaaacg ggtttgcgag ctgatgcaat cgcgcacggc
     3721 ggcgccggag caggccgtct ctctccagta cctctcgacg atggcccggc tctgtagccg
     3781 tgccgatttg ccgccgccgc ccaccagcga cgacgccaga cagaagtact ggacagactg
     3841 gcacaattac caggaacgca ttctcggagg ctccatatcc accctcgccg cgagcctgcc
     3901 ccggaagcag gccggcggga agaccatcgc gatcgaagcg ttgcttcagc ggatccagca
     3961 gcggcgaagt atagatccgc aaggaccggc cccggactgg attcctgttc tccgccagga
     4021 attcatcaag agcttccccg cgctcgagag ctggcggaag agtcatcctc tgacgatgta
     4081 tgccgcggtc ctcaaaggcc ccgagttgac ctcgctggcc gaatccatgc tcgacgcgtg
     4141 gaaacccggc gattactatg aaggcccgcg gtccgcgctg cacgttctgc acgcgctgga
     4201 cagcgtgcgt gcggaaaaac gaattctggc ggaggtggtg aagccgaaaa cgtggctcga
     4261 cacgccgcag ctcgaccttt tgccggccgc cgccgtgccg ccgatggatg atgaattgat
     4321 cgaagcactc gccgccgcgc agcgcgaagg gggctggaac ctgccattgc gaatggccgc
     4381 gttggcgaag tacgctacac cacgcgcgct ctcccgcatc caggcgatat acgagtctca
     4441 acaggtctcc tgccaaccgg agctgatggc gtatttcctt cgagcggagc cggcgtacgc
     4501 ggaccgcgtg ttccgaaacc accaatggga catgcaggcc gccccgccgc tatgcatctt
     4561 gcagtatttc gagcggactc cgcgtctggc gatgcacacg gtgctggaac gttacatggc
     4621 ggcttatctc atgcaccgcg acgtgccggt gaagaaaatg gcggcgtatt ccctgggctt
     4681 gtatggctcc aaagacgcat tgttcccatt gtgggaagcg ttccgctact ttcacgagta
     4741 ttggaaaggg aaaaagacgg agctggaaca aaatcaggaa ggcgtattcc tggaagtgga
     4801 gctgcgaaat gccatcgcac ggggccgcgg ctggctcgcc accgaaacgg acctgcgcac
     4861 aatagagtcg ctctgtatct cggaacgctg catctacgat acgcggcagg atctacagaa
     4921 cgtgcaggag ccgttccgca ttgaaatcac cggctcgggg ttcggcggct tgcagacgag
     4981 aatcgcgcaa tactacgggc ttaccggcat ggagcaggtg aaagacaagc tcgcgcaatt
     5041 tccgccgggc acccggttcg tgttggcggc gcacggcagc gatattgaca acgcgtccgc
     5101 cgaaatccga aaatatgcag ccgggcggcg tcttgtgatt gtgctcgggc ggcctgatat
     5161 gctctcacaa tgagcgccgg cgaacaaacg gattcgattc cgcctgcctg gcagttgctc
     5221 gaacgcgccg ccgcgaaaaa cctcttcgat tatgttcagg cgtttcgcct ggcaacgacc
     5281 gacgtacttg ccgccgccac cgactgcgcc ggaggcattg ctgcatatac gggcgccggc
     5341 tccccgctca ccactatcaa aggggccgga cccgatctct ctgacgacgc gctggatcag
     5401 gccgaggcct tcttccggaa atgcaactca ccctgcgtgg tgttcgaact tgctccgtgg
     5461 atcacgcccg ccgcggtcga acgccttacc cgcaggcaat atcaggtgag ggcggtggag
     5521 catgtaatgc tgctcgatgc gcccttcaag ataccggagc ctcagtcggg catcaggatt
     5581 ctgaacgccg ctgaatgggc cgatctggtg tttcgaggat tcgaggccgc ggatacgcct
     5641 gtctggcatc atctcgcgga ggcgagtgca cttcttccgg aagcggcgaa ccttggcgta
     5701 tggggcacct ccgcagatgc ttgcattgcc tgtgctcaga tggtccccgc ggctggtgtc
     5761 gccatattca gttgcgacgc gacgctgccc acggcgcgcg gcaagggcgc ccaaacggcg
     5821 ctgattcgtg aacggcttcg tttggcgagc gccatcggcg caacatgcgc tgccgctgag
     5881 gtagcgcccg atggcatctc ggaacgcaat taccttcgat gcggcttccg gctggcttac
     5941 agccgcaaac attatgttag gaacctgggt taaagcgaaa tgagtctatt tcatcgatct
     6001 gtggtggtgg ccgcgattac cgtcacggcg gctgctgccg ccgtgcgcgt cggaaccttt
     6061 ccgtcggaag tgcgaacgtt ttacaccacc ggtgacggac tgccatccaa tgatgttctt
     6121 gccgtttcta ttcacggcgg gaagttgttc gcacgcaccg ctgcgggcgg cgcggtgttc
     6181 ggcaacggca aatggattcg ttcggagtac ccggcggaga agaaacccga tctcgaaggg
     6241 ttgccaggcg gcgtttcgat tcggcagact gcgagtcatg ccgatggcag agtcgccgtc
     6301 gcagtcgaag aaggcctctt cctgcgcgaa ccctccggga aatggagtga cctctatcct
     6361 cacgaaggtt cccgaagctg gcatccgaaa gacgttcgtg gagtggcttt cgatgtgagc
     6421 ggccggtttt ggtttgcaag cccgcagggc gtaggccgct tcgacggaac atggaaactt
     6481 tacaccggtc acgatgggct tccgtacaac gacttcacca cgatggcgac tggtgagaag
     6541 ggagtggtct ggttcggcac aggccgcggc gccatccggt ttgatggaag cgtttgggag
     6601 taccggcaag gactgagatg gctgccatcc gatgaagtcc gaagcatcgc cgttgaggag
     6661 gatggcgatg cctggttcgc tacccccgct ggtgtcgggc tgatcgaaag acggtccacc
     6721 acgctttcac agaaagccag gttcttcgaa gacgacatcg acaaacgcca ccgccgcacg
     6781 ccttacggtt acgtggatag tgtcagcctg gagcgtccgg acgacctctc caaatggacg
     6841 cagcacgaca gcgacaacga tggactctgg acgtccatgt acggtgcggg cgaatgcttt
     6901 gcctatgcgg caacccgcga tcctttggcg aaaaagcgcg ccgatgcggc gtttcgcgcc
     6961 cttcgttttc taggcgaggt cacccgcggc ggcagtcatc ccgctccgcc gggtttcgtg
     7021 gcgcgcacga ttctgcccac cagcggtccg aatccgaata ctgtctacac gccggagcgg
     7081 gaccgcgagc ggcaggcaac gcgcgacgct ctatggaaag tcatcgtgcc ccgatggccc
     7141 accagtgccg acggcaaatg gtattggaaa accgacacga gctccgacga gctcgacgga
     7201 cactatttct tctatgcggt ctattacgat cttgtcgcgg cgaacgaaga cgagcgcagg
     7261 caggtacgcg atcacgtcgc cggtctcacg gaccatctca tccggcacgg atacaacgta
     7321 gtagatcatg acggcaagcc gacacggtgg ggtgtcttca gtccggagaa gctgaatcac
     7381 gatcctctct ggtttgaaga gcgcgcgctc aactcgctga gtattctttc gtacttgcgt
     7441 accgccgaac acatcaccgg aaatccgaag tacggcgagg cggcgcgcga gctgatgacg
     7501 aaacacgcgt atgcgatgaa cgtgatgatt gccaaaacga acgccggtcc gggatccgga
     7561 aatcagtccg acgacgagat ggcgttcatg aacttctact gtctgctcaa gtacgagaaa
     7621 gaccccaagc tgcgctcggc gttcgcgttc gcgttctcac ggcgctggat gatggagcgc
     7681 tacgaattga atcccttgtt caatttcatt tatgcagcgg tagcttctgg catcgagtat
     7741 gaaaacgctt tcgggcgggg agacctttct ccggcagggg actggctggc ggaatccatc
     7801 gacacgctga agcgctatcc catcaatcgg gtcagttggg ggctttccaa cagccatcgc
     7861 acagacatag tgttgttgcc cctttacgcg cgcgaggaag ggcagcgcgg aagccgccgc
     7921 gacggcaggg tgctgccgat cgacgagcga tttgtcgaca agtggaatca cgatccgtgg
     7981 gcgctcgatt accacggaaa cggacggcgg ctcgccgatg gcgcgtcgtt cctgctgccc
     8041 tattacatgg gcctgtatca cggcttcatc gccgattaag cgaaggaaga agccgtacgc
     8101 attcgcgcgc gatctgccac tcttcgtcgg tgcgaatcac cagcactcgt actcgcgact
     8161 gcggcgtggc gatgtcctgg tcaacgggcg aatcgccatt gtgcgcggca tccatttcga
     8221 cgccaagata acggaaccgt ttgcacaccg cttcgcgcaa cggtgcggag ttttcgccaa
     8281 cgccggcggt gaacgcaaga gcatccatgc cctggaggct ggcaaccatc ccgccgatct
     8341 cgcggcataa gcgttgtatg taagcgtcga atgccagccc ggcgcggcgg tttcccactt
     8401 cgacggcggc catgatttcg cgcatgtcgc ccgagatgcc ggagagtccc ttcagccccg
     8461 actc
//
LOCUS       DASTMK010000540         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_123834, whole genome shotgun sequence.
ACCESSION   DASTMK010000540 DASTMK010000000
VERSION     DASTMK010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123834"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="VFQ79_22365"
     CDS             <1..2004
                     /locus_tag="VFQ79_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU0142483.1"
                     /translation="NLLVNGSEGIAVGMATKIPPHNLSEIVDATVALIRDPSLPITEI
                     MKFVQGPDFPTGAYILGRSGIWDAFNTGRGSIRMRAKTHFEDIGRDRQAIVVTEIPFQ
                     VNKARLIEAAAALVNDKKLEGISEIRDESDRKGMRFVFELKRGENAEVILNNLYKHTQ
                     LQTSFGVIMLSIVNGQPRELGLIDCIKHFIDHRVDVVRRRTDYELRKARDREHILLGF
                     QKALDHLDEVIETIRRSDSPRDAKARLVERFEFTERQSQAIIELQLQRLTGMERQKIL
                     DELADIQVRIARYLEILGSEEVLRGVIVEELEEVKAKFGDARRTEIIEDPGDLNIEDL
                     IADENVVLTVSRSGYLKRVALDTYRRQRRGGTGRIGMTTRAEDLVEHLIVASTHTRLM
                     CFTNLGRVYINKVYEVPDAGPTGKGKHVSNLLNLMPGETVQAFLPVREFSEGKNVVMV
                     TRKGVIKKCTLTAFANINVRGIIAIGLDEGDELISARLTEGDAYIFLASRDGKAILFK
                     EDQVRPMGRPARGVRGMTLAKGDYIVSMAVPTDPETPILVIAEKGLGKRTPVKEYRET
                     GRGAKGVINMKTTSRTGKVVTALQLPIGPDGEPTQEVMVITVNGQIIRTGVDEIRKAG
                     RATQGVMVVRLDEGDVVAAAAIIPDEPGNGDELDTEQGTLPLQ"
     gene            complement(2087..3094)
                     /locus_tag="VFQ79_22370"
     CDS             complement(2087..3094)
                     /locus_tag="VFQ79_22370"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0142484.1"
                     /translation="MSRPTPMRIIQAVNAFQLTAAMKAAIDLRVFTAIGEGRLTAEAI
                     AEYCQTSPKGMRVLCDFLVVHEFLSKDGLRYGLAPDAAAFLDRNSPTYLGGIVNFQLG
                     DVQRKAYEDLVPVIRKGGTVLNEASGTMSKADPMWEEFARSMMSAVMPGAEAIVTLVN
                     ARSGVPMKVLDLAAGHGLFGITIAQHNPNATVYAVDWPSVLEIAREHALSAGVEERWN
                     GIKGNAFDVEFGQDYDVVLLTNFLHHFSSDMIEPLLRKVHGAMKPDGVVVTLEFVPNE
                     DRVTPRDVAAFAMVMLAMTAEGDAYPFSEYEAMFRNAGFQQNELRRIEGHRQAIILSR
                     R"
     gene            complement(3197..4081)
                     /locus_tag="VFQ79_22375"
     CDS             complement(3197..4081)
                     /locus_tag="VFQ79_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142485.1"
                     /translation="MSATAFSQETPPAAAPPHDEALPKLKTSRPAGQGGWKSVDATEA
                     AEGAPSNEAAKAPSSADAALEVPLGTRIPLSLINSVSTRSAAPGDRVYLETTFPIFAN
                     GKIVIPPGSYVLGTITTTKRPGRVKGKGEMHLRFDSITLPNGVTRDFRARVGGLDGGL
                     REGLDRDEGTVKGDSNKGSDTMTAVGTTVTGGQVGALGGLAAGNVGRGAGVGLAAGAA
                     AGLATVLLTRGPDVVLPSGTNLELVTDRPLTFTPEEIKFDGQMQRSIIRPAPAPEVNQ
                     RSTGRGGIGGIGGIGGPF"
     gene            complement(4286..>4560)
                     /locus_tag="VFQ79_22380"
     CDS             complement(4286..>4560)
                     /locus_tag="VFQ79_22380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU0142486.1"
                     /translation="VNILHDGQKSLSNQFATMNADRFSGVGWKAGKGGSPLLLDCLAQ
                     VECLTHKIVDAGDHAVFFGEVIGGDLGEGDPLLYFNRGYRTLAPTE"
BASE COUNT          951 a         1392 c         1319 g          898 t
ORIGIN      
        1 aatctattgg tgaatggttc ggaaggcatc gccgtgggca tggccaccaa gattcctccc
       61 cacaatttat cggagatcgt ggacgccacc gtcgccttga ttcgcgaccc gagcctgccc
      121 atcacagaga tcatgaagtt cgtgcagggc ccggacttcc ctaccggcgc ctacatcctt
      181 ggacgctcag gaatctggga cgccttcaac accggccgcg gaagcattcg catgcgcgcc
      241 aagacccact ttgaagacat tgggcgggat cgacaggcga ttgtggtgac ggagatccct
      301 tttcaggtga acaaggcccg gctcatcgaa gctgccgccg ccctggtgaa cgataagaaa
      361 ctcgaaggca tcagcgagat ccgcgatgaa agcgaccgta agggaatgcg cttcgttttc
      421 gaactcaagc gcggcgaaaa tgctgaggta atcctcaaca acctctacaa gcacacgcaa
      481 ctgcaaacca gctttggcgt gattatgctt tcgatcgtca acgggcaacc gcgggagctg
      541 ggcctgatcg attgcatcaa gcatttcatc gaccaccgtg tggatgtcgt gcgccggcgg
      601 accgattacg aattgcgcaa ggcccgcgac cgcgagcata tcttgctggg cttccagaag
      661 gcgctcgacc acctcgacga agtgatcgag accatccgcc gttcggacag cccgagagac
      721 gccaaagcgc gcctggtgga acgcttcgaa ttcactgagc gtcagtccca ggcgatcatc
      781 gaactgcaac tccagcgcct caccggcatg gagcggcaga agatcctgga cgaactggcc
      841 gacattcagg tgcgcatcgc gcgttacctc gaaattctcg gttcggaaga agtgttgcgc
      901 ggcgtaattg tcgaggaact cgaagaagtg aaggcgaagt tcggcgatgc gcgccggacg
      961 gaaatcatcg aagatccggg cgacctaaat atcgaagatt tgatcgccga cgagaatgtc
     1021 gttcttacgg taagccgctc gggttacctg aagcgcgtcg ctctcgacac gtaccggcgg
     1081 cagcgccgcg gtggcactgg ccgcatcggt atgacgacgc gcgccgaaga cctcgtggag
     1141 catctcatcg tcgcctccac gcacacaaga ttgatgtgct tcaccaacct tggaagggtt
     1201 tacatcaata aggtttacga agtgccggac gccggcccaa ccggcaaggg caagcatgtc
     1261 tcgaacctgc ttaacctgat gccgggagaa actgtacagg cctttcttcc cgtgcgcgag
     1321 ttctccgaag gcaagaatgt ggtgatggtt acgcgcaagg gcgtcatcaa gaagtgcacc
     1381 ctgacggcgt tcgcgaacat taacgtgcgc ggcatcattg ccatcggcct cgacgaagga
     1441 gatgaactca tctctgcccg ccttactgaa ggcgacgcct acatcttcct ggcctcccgc
     1501 gacggcaagg cgatcctctt caaggaagat caggtgcgcc cgatgggacg tcccgcacgc
     1561 ggagtgcggg ggatgacgct ggccaagggc gattacatcg tctccatggc ggttccaacc
     1621 gatccggaaa cgcccatcct cgtgattgcg gagaagggcc tcggtaagcg cacaccagtg
     1681 aaggagtatc gcgaaaccgg ccgcggcgcc aagggggtaa tcaatatgaa gaccacctca
     1741 cgcaccggaa aggtagtgac ggcgcttcaa ttgcccattg ggcccgatgg ggagcccact
     1801 caggaagtga tggtgatcac cgtaaacggc cagatcatcc gcaccggcgt ggatgagatc
     1861 cgcaaagccg gccgtgccac gcagggcgtc atggtggttc gtctggacga gggagatgtg
     1921 gtagctgcgg cggccatcat ccctgatgaa cctgggaatg gcgatgagct ggacaccgag
     1981 cagggaacgc tgccgctgca gtaacgcggg tacggcggat ataaaactag aagggcggct
     2041 ccggcgtgat tgccgttgcc gccctttatt tcttaacgcc caaccgttac cgccgcgaca
     2101 gaataatcgc ctgccggtgc ccttctatac gacgcagctc attttgttgg aagcctgcgt
     2161 ttctgaacat cgcttcgtat tcggagaacg gatacgcatc gccctccgcc gtcatcgcca
     2221 gcattaccat cgcgaaagcc gctacatcgc gaggagttac gcggtcttcg tttgggacaa
     2281 actcaagtgt caccaccacc ccatcgggtt tcatcgcacc gtgcactttc cgcagcagcg
     2341 gctcaatcat atcggagctg aaatggtgca ggaaattcgt gaggagcacg acatcgtagt
     2401 cctggccaaa ctctacatca aacgcgttac ccttgatccc attccatcgc tcctccacgc
     2461 ccgcgctcag cgcatgttcg cgggcgatct ccagcacgga gggccaatct acggcataca
     2521 cggtggcatt ggggttgtgc tgcgcgatgg tgatgccgaa gaggccgtgg ccagccgcga
     2581 gatcgagcac cttcatcggg acaccgctcc gggcattgac gagagtcaca atcgcttcgg
     2641 ctcccggcat cacggcgctc atcatggaac gggcaaactc ttcccacatc gggtccgcct
     2701 tggacatagt cccgctcgcc tcattgagca cggtgccgcc tttgcgaatc accggcacca
     2761 gatcctcgta ggctttccgc tgcacgtccc caagctggaa gtttacgatc ccaccgagat
     2821 aagtgggcga gttcctgtcg aggaacgccg ccgcatcggg tgcaagtcca taacggagcc
     2881 catctttact caggaactcg tgcacgacga ggaagtcgca gagaacccgc atacccttcg
     2941 gggacgtttg gcagtactcg gcgatcgctt cggccgtcag ccggccttct ccgatggcgg
     3001 taaacacccg cagatcgatg gctgccttca tcgccgctgt aagctggaat gcgttcacag
     3061 cttgaatgat gcgcatgggt gtcggtcgtg acatttgctt ttttctcgat tgtgcgtagt
     3121 tttcgacagg tctggcttgt gttagcgcat ctgcggcagg ttgacgttga agtcgtgaag
     3181 ctcagggcgc cgccgcttag aacgggccgc caatccctcc gataccaccg atgccgccgc
     3241 ggcccgtgct gcgctggttc acttcggggg caggcgccgg gcggattata ctgcgttgca
     3301 tctgtccgtc gaatttgatc tcttccggag tgaatgtcag cggccgatcg gtaaccaatt
     3361 ccaagttcgt accggaaggc aacacgacgt cggggcctct ggtgagcaac accgttgcca
     3421 gcccagcagc ggcgcccgcc gctaaaccga cgccggcgcc acggcccaca ttccccgcag
     3481 ccaagccgcc caacgcgccc acctgccctc cggtgactgt tgtgcctacc gccgtcatcg
     3541 tatcggagcc tttgttcgaa tcgcccttca ccgtgccctc gtcgcggtcg agcccttccc
     3601 tgagcccgcc atccaagccg ccaacgcgcg cgcggaaatc ccgcgtaacg ccattgggga
     3661 gcgtaatgct gtcgaagcgc agatgcatct cacccttgcc tttaacgcgc cccgggcgct
     3721 tcgtggtggt gatcgtgccc agcacgtagg atcctggcgg aatcacaatc ttcccgttcg
     3781 caaaaatggg aaacgttgtt tcgagataca cacgatctcc aggcgcggcg ctgcgcgtgc
     3841 tgacgctatt gatgaggcta aggggaatgc gagttcccag aggcacttcc aaggcggcgt
     3901 ccgctgacga tggggcctta gccgcttcgt tcgatggggc gccttccgca gcttccgttg
     3961 cgtcaacgga cttccatccg ccctgcccgg cgggacgcga agtcttcagc ttgggaagtg
     4021 cttcgtcatg aggcggcgca gcggctggcg gagtttcctg agagaaggcc gtggcgctca
     4081 ttgccaaagc cagggcaatt acagtgaagg tccgtagagc aataccactc attgatccgg
     4141 ccatcctttc gagggttcat gcattcacgc caattctaat cccgctcgtg ctacgccgag
     4201 atagttgacc gggcgcaagc gtgctccgat gcaaactctg ctacctactc gaacgtattt
     4261 cgcggcttga cggtttcgcc ccggcctatt ccgttggcgc taatgtacga tagccgcggt
     4321 tgaagtaaag tagcggatcg ccttcaccca aatccccacc gatcacttcg ccgaagaaca
     4381 ccgcgtggtc gccggcatcg acaattttgt gggtgaggca ttccacttgc gccaggcaat
     4441 ccagcaacag gggcgaaccg cccttgccag ccttccaccc aactccagaa aaacggtcag
     4501 cattcatggt ggcgaattgg tttgagagcg atttctgtcc gtcatgcaga atattcaccg
//
LOCUS       DASTMK010000541         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_379787, whole genome shotgun sequence.
ACCESSION   DASTMK010000541 DASTMK010000000
VERSION     DASTMK010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379787"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFQ79_22385"
     CDS             <1..876
                     /locus_tag="VFQ79_22385"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0142487.1"
                     /translation="MIPKLFVALISFAFLPVLAAQPQILDAHVHHNGDPAFLKALIKR
                     LDPFNGLAFVLVKPEHLAATKPIIDAHPNRLVGFGDIDLDDPEAVKRVDEFHAAGFRG
                     IGELSRPLKNFDDPKYSPIYARAEQYGMIALFHTGIVNRTTPDIPEDVSVDRMRVLLL
                     DGIARRYPKLTIIGAHLGNPEYANAAEIARWNPNLYFDLTGSTLIKKQEDYTFFKSVF
                     WWSSVASPHTPASGASAFEKIVFGSDIFDGEIEEFDRSLDRYRKMLSACGVSKEAQAN
                     IMSGTMWRILNRSSK"
     gene            1022..2032
                     /locus_tag="VFQ79_22390"
     CDS             1022..2032
                     /locus_tag="VFQ79_22390"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0142488.1"
                     /translation="MPGPYRGRVAAAHHPGSILSGQYQREPVRAMLSKTMMELTGAPS
                     AQEAWRVFFQPGDVVGIKLNPVGRPHVRSSPEVVQEIVAGLEMAGVKRRDIVAYDRYR
                     REFLDAGFDKWLPEGVRWTAATDKTHPLQLDMDGYDADQYIEMSLVLPKANPSDPHHR
                     RSYVAKFLSKEVNKMVNLCVLKHHQSAGVTMALKNMSHGLVNNVSRSHSSSTLNACGT
                     FIPTVVDHPIIRQKCVLHILDAVKAAYHGGPGGRVGKYMWEHKTMYAATDPVALDRVG
                     WKVIDAKRAEVGRQPIALAKPDQDSRFLNMQVEHIEIAGALGLGEFRDEAIDLRSFNL
                     TS"
     gene            2219..3103
                     /gene="pdxS"
                     /locus_tag="VFQ79_22395"
     CDS             2219..3103
                     /gene="pdxS"
                     /locus_tag="VFQ79_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU0142489.1"
                     /translation="MYLDDSFRVKVGLAEMLKGSVIMDVTNAEQALIAEKAGAAAVMA
                     LERVPSDIRKEGGVARMAPIRKIREIMATVSIPVMAKARIGHFMEARILEALEVDYID
                     ESEVLTPADEEHHIAKRDFKVPFVCGARNLGEALRRIAEGAAMIRTKGEAGSGNIVEA
                     VRHIRTIVKEMKQLTVLGKEELVSESKKLQAPLELVQWVAENGKLPVPNFSAGGIATP
                     ADAALVMQLGAEAVFVGSGIFKSEDPEARARAIVKAATYYNDPVKLLEASEELGMAMP
                     GLDVSKLQETELLQTRGW"
     gene            complement(3126..>3521)
                     /locus_tag="VFQ79_22400"
     CDS             complement(3126..>3521)
                     /locus_tag="VFQ79_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142490.1"
                     /translation="RELMITALIRGRLCELLAPSAGMESRKSELFLMGLFSLLDAILE
                     QPMKTLLEEIPLAADVRSALLGEPSGSPVARLHNLLEAYERGQWDATTRLSAQLGIDE
                     TALPALYVDAVAWAEECSVQHAQTTPVTR"
BASE COUNT          761 a          986 c         1058 g          716 t
ORIGIN      
        1 atgataccga aactgtttgt tgcgctgatt tcatttgctt ttctgccggt actggctgca
       61 cagccgcaaa ttctggatgc gcacgtgcac cataatggcg atccggcgtt cctgaaagcc
      121 ctcataaaga ggctggaccc gttcaacggg ttggccttcg tgctggtgaa acccgaacat
      181 ctggcagcca cgaagcccat tatcgacgcg cacccgaacc gcctggtggg attcggagac
      241 atcgatcttg acgatccgga ggcggtgaag cgcgtagacg agtttcacgc ggcgggtttc
      301 cggggcatcg gcgaactgtc cagacctttg aagaatttcg acgatccgaa atactcgccg
      361 atatacgccc gcgcggaaca atacgggatg atcgcgctct ttcatacggg gatcgtgaat
      421 cgtacgactc ccgacatccc ggaagatgtc agcgtcgatc gcatgcgcgt tctgctgctc
      481 gacggcatcg cacggcgcta cccgaaattg accattattg gagcacatct cggaaacccg
      541 gaatatgcca atgcggccga aatcgcgcgt tggaatccga acctttactt cgacctgaca
      601 ggatcgactc tgatcaagaa gcaggaagac tatacttttt tcaagtctgt cttctggtgg
      661 tccagtgttg cgagcccgca cacaccggcg tcgggagcga gcgcgttcga gaagattgta
      721 ttcggttcgg atatcttcga cggcgagatc gaggagtttg accgctcgct ggaccgttat
      781 cgcaagatgc tgagcgcctg tggcgtgtcc aaagaagccc aggccaacat catgagcggt
      841 accatgtggc ggattctgaa tcgtagcagc aaatagtccg ggctgttaga attggggaaa
      901 acctcttaga gggggatttc gaatggatca ggcacgcagg gcattccttc ggaatgcggt
      961 atggacagcc gctgcggctc aggccgcgag ttccgcacca caagcggcgg gaaaacctgg
     1021 tatgccggga ccctatcggg gccgggttgc cgcagctcac cacccgggga gcattctttc
     1081 gggacagtac cagcgcgaac cggttcgcgc catgctgagc aaaacgatga tggagctcac
     1141 cggagcacca tcggctcagg aagcctggcg cgtgtttttc cagcccggcg acgtggtcgg
     1201 catcaaactg aatcccgtcg ggcgcccgca tgtcaggtct tcgccggaag tggtccagga
     1261 gatcgtggcc gggctcgaga tggctggcgt gaagaggcgc gacattgttg cgtacgaccg
     1321 ctaccggcgc gagttcctgg atgccggctt cgataaatgg cttcccgaag gcgtccgctg
     1381 gacggccgca accgataaga cacacccttt gcagctcgat atggacggtt acgacgccga
     1441 ccaatacatc gagatgtcgc tggtgttgcc gaaggcaaac ccatccgacc cgcaccaccg
     1501 ccgctcgtat gtagcgaaat ttctttcaaa agaagtaaac aagatggtga atttgtgcgt
     1561 gctgaaacac caccagtccg ccggagtcac catggcgctg aagaacatgt cgcatgggct
     1621 ggtaaacaat gtcagccgca gccactccag ttccactttg aacgcatgcg gcacgttcat
     1681 tcccaccgtt gtggaccatc ccatcatccg ccagaaatgc gtattgcata ttctggatgc
     1741 ggtcaaagcc gcgtatcatg gcgggccggg cggaagggtt ggcaagtata tgtgggagca
     1801 caagaccatg tatgcggcaa ccgatccggt tgctctcgac cgcgtagggt ggaaggtcat
     1861 tgacgccaaa cgcgcggaag taggcaggca gcccattgca ctggccaaac cggatcagga
     1921 tagccgtttt ctgaacatgc aagtggagca tatcgagatc gccggtgctc tcggtttggg
     1981 cgagttccgt gacgaagcca tcgatcttcg cagcttcaac ctgacgagct gagccacccc
     2041 gttttttgtc cggaagtcat gagcggaagg cgcgcgcgag aaggatcgcc gtacctgcat
     2101 ccgtgtgcta ggattgaatc aactaatcga gtttcttccg gtaccgtaag gtcagcccag
     2161 ccggtatttt ccgggtgcgc tcgcttttgg ttggggtgtt ttggaggaga agtttgcaat
     2221 gtatttggat gattcgtttc gagtaaaagt cggcttggcc gagatgctca agggcagcgt
     2281 catcatggac gtgacgaacg ccgagcaggc gcttatcgct gagaaagcag gcgccgcggc
     2341 tgttatggcc ctcgagcggg ttccctctga catccgcaaa gagggtggcg ttgcgcgcat
     2401 ggctcctatc cgcaaaattc gcgagatcat ggcgacggtt agcatccccg tgatggcgaa
     2461 ggcgcgaatc gggcacttta tggaggcacg cattctcgag gcgcttgaag tcgattacat
     2521 cgacgagagt gaagtcctca cacccgccga cgaagaacac catattgcta aacgcgactt
     2581 caaggttccg ttcgtctgcg gcgcccgcaa cctcggcgag gcgctgcggc gcatagccga
     2641 aggcgccgcc atgatccgta ccaagggcga ggcgggttcc ggcaatatcg tcgaagcggt
     2701 gcgccatatc cgcaccatcg tcaaggaaat gaagcagctt acggttctgg gcaaagagga
     2761 actggtaagc gagtccaaga agttacaagc cccgctcgaa ctcgtccagt gggtagcgga
     2821 gaacggcaag ctaccggtac cgaacttttc cgcgggcggc attgccaccc cggccgatgc
     2881 cgcgctggtc atgcaactcg gagcggaagc ggtattcgta ggctctggca ttttcaaatc
     2941 tgaggacccg gaagcccgcg cccgggcgat cgtgaaggcc gctacgtatt acaacgatcc
     3001 ggtgaagctt ctggaagcga gcgaggaact cggcatggcg atgccgggac tcgacgtttc
     3061 caagctccag gaaacagagt tgctgcaaac ccgcggctgg tagttggcgt cttcggcggt
     3121 gacccttatc tggtgacagg tgttgtctgc gcgtgctgga cactgcattc ttcagcccac
     3181 gctacggcat cgacgtagag tgccggcagt gctgtttcgt cgattccaag ctgcgcgctc
     3241 agcctcgtcg tagcgtccca ctgtcccctc tcgtaagctt ccagcaaatt gtgtaaccgc
     3301 gctaccgggg agccggatgg ctcaccaagc agggcggagc gtacgtccgc cgcaagaggt
     3361 atctcctcca gcaaagtttt catcggctgc tcgaggatgg cgtccagcag tgagaaaagc
     3421 cccatcagaa acaactccga tttccggctt tccatcccgg ctgaaggagc gagcagttcg
     3481 cacaacctgc cgcggatcag tgccgtaatc attaactcgc g
//
LOCUS       DASTMK010000542        12237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_603965, whole genome shotgun sequence.
ACCESSION   DASTMK010000542 DASTMK010000000
VERSION     DASTMK010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603965"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFQ79_22405"
     CDS             <1..434
                     /locus_tag="VFQ79_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142491.1"
                     /translation="IGECLNYTLDPTSGRTQLRRLFQRVYNALKPGGLFVFDIAQPGQ
                     LAPGAQRREHWQGEDWAVLLQVTEDRRRHLLTRAITTFRKAGRLFRKSQEVHVLRLYK
                     GPAILAELERTGFRVRTLRGYGTARLPRTHIAFMARKPNTK"
     gene            472..1740
                     /locus_tag="VFQ79_22410"
     CDS             472..1740
                     /locus_tag="VFQ79_22410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU0142492.1"
                     /translation="MTVAPVVSESRVRYAAAAIFMQILLGVLYSWSVFRVPLAQLHGW
                     TKAETIAPYRYSLLVFAVGMILAGFWQDRKGPRIVATAGGFLLGTGCLLAAWIGETVS
                     GLVIAYGLVAGLGVGFAYVTPIAMCIKWFPDRRGMIVGLAVMGFGVGPLVFGPLLEAL
                     IGGDPSRFAETIPRTFLILAGIFYVGVIGAAQFYRVPPAGWKPTGWVPPPGRAAAIEF
                     PPSQMLRTWQFYALWLLYFLGTSVGLTAIGEATPLLQEMASATAVMSAGTALGIMSIF
                     NGAGRLAWGSVSDRIGRKSALLAMCLVSVIACLGFLRTASGFWPLLTGLCLAAFAYGG
                     YLALMPSFTADYYGPKNVGANYGLLFSAWGACGFLVPGYFAQIMDAARISGDLASGYR
                     EVYWKLALLAAVGAFAAALLRMPARRSSRN"
     gene            complement(1754..3787)
                     /locus_tag="VFQ79_22415"
     CDS             complement(1754..3787)
                     /locus_tag="VFQ79_22415"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0142493.1"
                     /translation="MDSAVTTSPPNDSIIVPHDVTLELYRKMLTVFYIEERMKTFVKQ
                     GKCSFQASTRGHEKVQIGMTMLLRPGHDWFFTYYRSKAIAVGLGMPLKDIFLGMLSRE
                     GDPNSNGRNMSEHWSSRKLKLVAQTAVTGTQYLNAVGMARAMKLGGGDEIVHVASGDG
                     ATSEGEFFEALNWAHREKLPVIFLVQNNGYAISTQQHHQTASEIHRIAGGFGMPSYSI
                     DGTWFEPMYQTLPAAIEAVRSGAGPVLIEASVIRLDPHSSSDDHRKYREQTELEELRG
                     RDPIWQTEQYIRRHELLTGEEVAAWRTSVKAEVDYAAEEADAHPAPSAVHVMAHIYAD
                     DPVVLGEPSEPRSVSDKPVTMIDAINHGLREELERNSKVVMWGEDIADPKGGVFGVTR
                     GLSTDFPGRVFNAPLAEASIAGVAAGMAIGGYKPIVEMQFADYLWPAALQLREEIPTV
                     RWRSQGEWECPVVVRAAVGGYIKGGPWHSTNVESFFSHIPGWYVVYPSNAADAKGLIK
                     AAARAKDPVVFLEHKGLYRKVQAKSLEPDSDYILPFGHGRVVLPGSDVTVVTWGATVY
                     HALEAARQMNTEGSSIEILDLRTLVPLDEELILRSIRKTNRVVIAHEDTLTMGFGAEI
                     AARIAEHFLDVLDAPVVRVAAKDSFVPNAPNLEAEVLPGVEDLRRGIERVLNY"
     gene            3909..5960
                     /locus_tag="VFQ79_22420"
     CDS             3909..5960
                     /locus_tag="VFQ79_22420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142494.1"
                     /translation="MGAFRLLFATVCMGGIAVYPQRVGTPTGDLTNLSVEELFSVQVT
                     SVGRKAQQLSRAPAAVYVLSAEEIRRSGATSIPEVLRWVPGLTVSRLDGRSWVVSARG
                     SGRLYSNKILVMIDGRSLYTPLFSGVVWDSIDVPLEDIAQIEVIRGPGTVVWGPNAVN
                     GVINIITKRTKATTGTEVSVAAGNELRTGLFVRWGDVPRDTLSYRIWGKLEQRTPSFS
                     SPGYYFHNNKYVYRVASLDGLDSTTGRAGFRLDWQPNKTDEWLLQGDIYKLSREDPVT
                     YPILLPGLVEFSAERARYEGGSIQGRWNRTRAPGMEDSVQFSFDRNELGFPYLTARLN
                     NVTIDYQRRRIIGERNELYWGGGYQQYWDSTVSRRSIWFTPEDSSYRAGNVIVRDEIQ
                     LLPGRLLLSAGIRLDYNSYGNFEHQPSFRLLYTPNAKQSAWFGFSRAVRVPSRYDRDL
                     RTASGREYIGDVPFWVGLAGSRTTRAEVERSLEAGYRLQSGQRWSIDVALFRSLYRNL
                     RILRMPPMPEGGSPVSAPPAPLPLVQESAGLGRSYGGEFSATWHVRPSWKLAPAYSYL
                     NQNRWLPHTAANWYAWEDCMGGSPHQVLLRSSHDLSERWQLDLMARARSRNKGYDLPG
                     AVLADARVSWRLTRSGELSISLQNLTNRQAVESYSEPPYAAIPLRRTFVVKWTHKFFA
                     D"
     gene            5978..6520
                     /locus_tag="VFQ79_22425"
     CDS             5978..6520
                     /locus_tag="VFQ79_22425"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEU0142495.1"
                     /translation="MPGSRLTAYIAIASMLALSALKAAEPEDELKAAVVLSFIRHAEW
                     KPSRAPGITIRVGVTGRPGFREALKRTLEGKTVGNSLVRVIDLETDMDTPCCHIVYFA
                     TTKNSEAKHRLSMPWAARAITIGEADQFIENGGAVAVHLMDGRIRFEVNLREVQRVGA
                     SISSKLLRFGRIRVPVGGGG"
     gene            6517..8448
                     /locus_tag="VFQ79_22430"
     CDS             6517..8448
                     /locus_tag="VFQ79_22430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0142496.1"
                     /translation="MRRSSSLRSKFMALMLGGSVAAAVLAAGGFSWLDLNRFWARTTD
                     EISAITSIVADQVGPAITLRDAKAAREILGSLRAVGIIRHAAIYDKHDRCFAVFQRDS
                     SSICPPRPQDGTTYGPGTMIVSRGVFSDGERLGTLALSATVPSLSKVLRQYLDGSLIV
                     LLLSLMAATVLSVTLQARILTPVLKIAEIAGRIARTHGYGERINVASRDELGVLASSL
                     NAMLDEIQHRDAELQRHRDRLEEEVAERSRVNIELRQAKQRAEDAARLKSEFIANTSH
                     EIRTPMNGVIGMIGLALETSADPEQREHLENALNAAKSLIVVLNDILDFSKIEAGKMT
                     IESIPFQLRKVLSESLRLFECTLREKNLGVQFTVGQECPEWVRGDPTRLRQIIVNLVG
                     NSVKFTPAGSIRLAVTTLPSDRIRFEVQDTGVGIAAEKIGLIFDAFTQADGSHTRQYG
                     GTGLGLAITKRLVSLMGGTIRARSELGYGSCFTFELSLPEAYDNREDEELGTTCKLTP
                     GLEHLRVLVAEDNIINQKVICSMLERLGWYVDLAENGKQAVALFRDREFDLLLMDIQM
                     PEMDGLEAARRIREEERGRNGKRTPMIALTAHTGQSQQEECMSAGMDAVVTKPFELQS
                     LLESLNAVRAASAVTSEQA"
     gene            complement(8429..9208)
                     /locus_tag="VFQ79_22435"
     CDS             complement(8429..9208)
                     /locus_tag="VFQ79_22435"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0142497.1"
                     /translation="MKPLLCLCIGLVPLFAEPVAAIRPKEAVQLFNGHDISNWYTWLR
                     ENRYEDPKRVFSVKDGLLRISGEEWGGIVTRQSWKDYHLVVEWRWGGPTWGSRKDRAR
                     DSGILVHAVGEDGAAGGIWLESIEAQIIEGGTGDFILVGGKNRPSLTVESREGKGGEL
                     YWQEGGEPRTRDRGRFNWFGRDPDWVDRIGFRGPNDVEKPVGEWNRQEVVADGGTLTN
                     ILNGAIVNRGTKSSHREGKIQIQSEGAELLIRKVELKPVQR"
     gene            9386..9907
                     /locus_tag="VFQ79_22440"
     CDS             9386..9907
                     /locus_tag="VFQ79_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142498.1"
                     /translation="MKHAIVPGVILVHRLAGSLLLSFCLVSPSHTETRGTEPEGAARK
                     WTTIKKSPRKAAETEGKWEIFKDITVDAGKSVVLDSSIDYSSLDKVAVAVRSASDSLS
                     DLESLTLQTFWSVPDADYFSAAEIKFGKTFPFTNSGSVLFGVYGSEFRLILRNEGALS
                     LKIAQLFIFCRSQ"
     gene            complement(9914..>12237)
                     /locus_tag="VFQ79_22445"
     CDS             complement(9914..>12237)
                     /locus_tag="VFQ79_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0142499.1"
                     /translation="LTALNPQQREAVEHVEGPLLILAGAGSGKTRVITHRIAHLIRTR
                     GIPGYQVLAVTFTNKAAGEMRDRVLNLAGSQDPRSGPVVSTFHSFCVRLLRRDGDPLA
                     AVRSNFTRNFTIYDEDDQLALIKAIFRHAGLDEKFMPYRSVLSRISHAKSQNQTPADL
                     YSASTDPKSSRLASLFEMYEERLQQANALDFDDLLLEAVRLLRIDEPTRAAYNRRFGF
                     VMIDEYQDTNRSQYDLMRLLTQAHQNICVVGDEDQSIYGWRGADIRNILDFERDFPNA
                     KVIRLEQNYRSTKNILESASALVANNTARKGKWLWTQSGAGEPIGFYEAWDGENEALF
                     IADTIEQMFSRNPAERIAVLYRTNAQSRQIEEALRRYGRKYLVIGGFSFYQRAEVKDL
                     LAYLRASVSPHDSIALLRIINTPARGIGRSTIEQLERFATEHNLSLWSAVDRMIEEKQ
                     LSTRAEAALSSFRRLLEELTEMAALGAGDLLKAVIDRTGYMKMLETDSSPEAESRIAN
                     VNELLNAAVDAAERGETVTEFLDHAALVADADGLDEHAAVSLLTIHNAKGLEFPVVFM
                     AGVEEGLFPHSRSVTSEAALEEERRLCYVGMTRAEKRLFLTWARSRRRWGGGQPEPCI
                     PSRFLAEIPREFVQALHRSRRETPQVDLFVEQHEVRQSAKRNTYTGKTYNSLDNIAQF
                     FTERGITPPPLNRTTAPPGPARTAQTAKPAADRSGAGLAQGFRKGSIVYHPTYGRGQV
                     VRLEGEGDDTKLTVSFRAGLKKIIAKYATLKTE"
BASE COUNT         2393 a         3626 c         3594 g         2624 t
ORIGIN      
        1 ccatcggaga gtgcctcaac tacacgctcg atcctaccag cggcaggacg caactgcgcc
       61 ggttgttcca gcgggtgtac aacgccctga aaccgggcgg gctgtttgtg tttgacatcg
      121 cgcaaccggg ccagcttgca cccggcgcgc agcgcaggga acactggcag ggagaagact
      181 gggccgttct gcttcaggtg acagaggatc gcaggcggca tctgcttacc cgcgccatca
      241 cgaccttccg caaagccggg cgcctgttcc ggaaaagcca ggaagtccat gtgcttcgct
      301 tgtacaaagg acctgcgatc ctggccgaac tcgagcggac aggctttcgg gttcgcacgc
      361 tgcgcggcta tggaaccgcc agattgccgc gaacacacat cgcgttcatg gcgcgcaagc
      421 caaacacgaa ataaaacgat tcccttggcg ttcgctgcta gaatatggcg aatgactgtt
      481 gcgcccgtcg tctcggaaag ccgtgtgcgt tatgccgccg ccgctatttt catgcagatt
      541 ctgctgggag tcctttactc ctggtccgtg tttcgtgtcc cactcgctca actgcatggc
      601 tggacaaaag cggagactat cgcgccgtat cgctactcgc tgctcgtatt cgcggtgggc
      661 atgattctgg ccggattctg gcaggatcgc aagggaccgc gcatcgtcgc caccgcgggg
      721 ggcttcctgc tcggaaccgg atgcctgctg gcggcctgga tcggcgagac ggtctccgga
      781 ttggtgattg cgtacggtct ggtagccgga cttggcgtcg gcttcgctta cgtcacaccg
      841 atcgcgatgt gcatcaagtg gtttccggac cggcgcggca tgatcgtggg cctggcggtg
      901 atgggcttcg gagtggggcc gctggtattt ggcccattgc tcgaagcatt gatcggcggc
      961 gacccctcgc gatttgccga gaccattccg cggacatttc tcatccttgc cggcatcttc
     1021 tacgttggcg tcatcggcgc ggcccaattc tatcgtgtgc ctcccgcagg ctggaagccc
     1081 acaggctggg tgcctccgcc gggacgggct gcggccatcg agtttccgcc ttcccagatg
     1141 ctgagaacgt ggcagttcta tgcgctatgg ctgctctact tcctcggcac ctcggttggc
     1201 ttgacggcaa tcggcgaagc cacccccttg cttcaggaaa tggccagtgc taccgcggtt
     1261 atgtcggccg gaacggcgct gggcattatg agcatcttca atggggcggg aaggctggcc
     1321 tggggtagcg tttcggaccg gatcggccgg aagagcgccc tgcttgccat gtgcctggta
     1381 tcggtaattg cctgcctcgg cttccttcgc acggcttccg ggttttggcc gctgctcacg
     1441 ggattgtgcc tggccgcgtt cgcgtatggc ggctacctgg cgctcatgcc ctcctttacg
     1501 gcggactact acggtccgaa gaacgtcggc gccaactatg ggcttctctt ttcagcctgg
     1561 ggagcatgcg gcttcctggt gccgggttat ttcgcacaaa tcatggacgc cgcccgtatc
     1621 tcgggcgacc tcgcctccgg ttatcgcgaa gtgtactgga aactggcgct gctggccgcg
     1681 gtaggagcgt ttgcggccgc gctgctgcgt atgccggcac gccggagttc acggaactga
     1741 tatcgggctc agctcaatag ttcagcacgc gttcgattcc gcggcgcagg tcctccacgc
     1801 ccggcagcac ctcggcttcc aggtttggag cgtttggcac aaaggagtcc tttgcggcga
     1861 cacgaacgac aggcgcatcc agaacatcaa ggaaatgctc ggcgatacgt gccgcgattt
     1921 ccgcgccgaa ccccatcgtc agcgtgtcct cgtgtgcaat gaccacccgg ttggttttcc
     1981 ggatgcttcg gaggatgagt tcttcatcga gcgggaccag tgtccgaaga tcaagaattt
     2041 cgatcgaact tccttccgta ttcatctgcc gggcggcttc cagcgcgtga tagaccgttg
     2101 cgccccaggt gacgaccgtc acgtccgagc cgggaagcac aacgcggccg tggccaaacg
     2161 gcagaatata atcgctatcc ggctccagcg acttggcctg aaccttgcgg tacaagcctt
     2221 tgtgttccag gaacacaacc ggatccttgg cgcgcgcggc cgccttaatc agtcccttgg
     2281 catccgctgc gttgctgggg tacaccacgt accatcccgg aatgtgcgaa aaaaaggact
     2341 ccacgttcgt cgaatgccat ggaccgcctt tgatatatcc gcccacggcc gcgcgcacca
     2401 cgacgggaca ctcccattcg ccctgactgc gccagcggac cgtcggaatc tcttcccgga
     2461 gttgaagcgc cgctggccac aaataatcgg cgaactgcat ttcgacgatg ggcttgtaac
     2521 cgccgatagc catacccgcg gccactccgg caatactggc ttccgccaga ggcgcgttga
     2581 aaacacgtcc gggaaaatcc gttgagagcc cgcgggtcac gccaaagaca ccgcccttcg
     2641 gatcggcaat gtcctccccc cacatcacta ccttgctatt acgctccaac tcctcccgca
     2701 ggccgtggtt gatggcgtca atcatcgtaa cgggtttgtc cgaaacagaa cgcggttcgg
     2761 acggctctcc aagcacaacc gggtcgtccg cataaatgtg cgccatcacg tggacggccg
     2821 agggcgccgg atgcgcatcc gcctcctccg cggcgtaatc cacttccgcc ttcacactgg
     2881 tcctccaggc ggcgacttcc tccccggtca acagttcgtg ccgccggatg tactgctccg
     2941 tctgccagat cggatcgcgc cctctgagtt cttccagttc cgtctgctca cggtatttcc
     3001 ggtgatcgtc ggaagaggaa tgaggatcca gccggatgac actcgcctct atcaggaccg
     3061 gtccggctcc cgaccggacg gcctcgatcg ccgccggaag cgtctggtac atgggctcaa
     3121 accaggttcc atcaatgctg tagctgggca tcccgaatcc gccggcaatc cggtggatct
     3181 cggaagcggt ctggtgatgc tgctgggtgc tgatggcgta accgttattc tgcaccagga
     3241 aaatgactgg cagcttctcc cggtgtgccc agttcagagc ttcgaaaaat tcgccctcac
     3301 tggtagcgcc atcgccggaa gcgacatgga cgatttcgtc gccacccccg agtttcatcg
     3361 cgcgggccat accgacggcg ttcaggtact gcgtgccggt gacggccgtc tgagccacca
     3421 gtttcagctt ccgcgaggac cagtgctcag acatgttccg tccgttcgaa ttcggatcgc
     3481 cctcccggct gagcattccc aggaagatgt ctttcagcgg catgcccagc ccaaccgcga
     3541 tggctttgga ccggtaatag gtgaaaaacc agtcgtgccc cggacggagc agcatcgtca
     3601 tcccgatctg tactttttca tgacctcgcg tcgaggcctg aaacgagcat ttcccctgtt
     3661 tgacgaacgt cttcatccgc tcttcgatgt agaagacggt aagcatcttc cggtacagct
     3721 ccagggtgac gtcgtgcgga acgatgatgc tgtcattcgg aggtgacgtt gttactgctg
     3781 aatccatacg ttgtgtccct gatttcagac tacaatatgc gaccggacaa gtccgtgaca
     3841 caagttacag gggagtcttc gatggcgcgc ggctaaaggg aaccggccgt tttaccgaat
     3901 agtgaagcat gggcgctttt cgtctactgt tcgccacggt ttgcatgggt ggtatagcag
     3961 tctatcctca gcgggtcgga acgcccacgg gcgacctgac taatttgagt gtcgaggagc
     4021 tctttagcgt ccaggtgacc tctgtggggc gcaaagcgca gcaattgtca cgggctcccg
     4081 cagccgtgta cgtactcagc gcggaggaaa tccgaaggtc gggagcgacc tcgatcccgg
     4141 aggtgctccg gtgggtgccc ggtctaacgg tttcacgtct tgatgggcgc tcgtgggtgg
     4201 tatcggctcg gggatcgggg cgcctttact cgaacaagat cctggtaatg attgacggga
     4261 gatccctgta tacgcctctg ttttccggag tggtctggga ttccattgac gttccgctgg
     4321 aggacatcgc tcagatcgag gtgatacgcg ggccgggaac ggtggtgtgg gggccgaatg
     4381 ccgtcaatgg agtgattaac atcatcacaa aaaggacaaa ggctaccacg gggacggagg
     4441 ttagtgtcgc ggctggtaac gagttgcgga cgggtttgtt cgtccgctgg ggtgatgtgc
     4501 cgcgcgacac gctctcctac agaatctggg gcaagctgga gcagaggacg ccaagctttt
     4561 catcgccagg ctattacttc cataacaaca aatatgttta cagggttgcc tctttggacg
     4621 ggctggactc caccaccggc cgtgctgggt tcagactcga ctggcagccg aacaagacag
     4681 acgagtggct gcttcagggt gacatctata aactgagccg cgaagatccg gtaacttatc
     4741 cgatattgct gccgggcctg gtggagttct ccgcggaacg cgctcgttat gagggcggct
     4801 ccattcaagg ccgctggaac cgtacaaggg cgcccggcat ggaagacagc gttcagtttt
     4861 cttttgatcg taatgagctg ggctttccat atctcaccgc gagactgaac aacgtcacga
     4921 tcgattacca aaggcgccgc atcataggag agcgcaacga actttattgg ggcggcggtt
     4981 accagcagta ttgggattcc acggtgagcc ggcggtccat ctggtttaca ccggaagact
     5041 cgtcgtatcg agccggtaac gtcatcgtaa gggatgagat acagctcctg cccggacgtc
     5101 tgcttctttc ggccggcata cggctggatt acaactccta cgggaatttc gaacaccagc
     5161 ccagcttccg cctactctac acgccgaatg cgaaacaatc agcctggttc gggttctcgc
     5221 gggcggtgcg ggtgcccagc cgctacgatc gggatctgag aaccgcctcc ggccgggaat
     5281 acatcggaga cgttccattt tgggtcgggc ttgccggctc acgaacaacg cgcgcggaag
     5341 tcgagaggag tcttgaggcc ggataccgcc tccaatcggg ccagcgttgg tcaattgatg
     5401 ttgcgctctt caggagcctg tatcggaact tgcgaatctt gcggatgcca cccatgccgg
     5461 agggtggctc accggtgagc gcaccgccgg cgcccctgcc actggtgcag gaaagcgccg
     5521 gtcttggacg gtcgtatgga ggggagtttt ccgctacctg gcacgtgcgt ccatcctgga
     5581 aactcgcacc ggcttattcc tatctgaatc agaatcgctg gctgcctcac acagccgcta
     5641 attggtacgc ctgggaggat tgcatgggcg gttctcctca tcaggtgctt ttgcgctcga
     5701 gccatgatct ttcggaaaga tggcaactgg acctgatggc aagggcccgc tccagaaaca
     5761 agggatacga tctgcccggc gccgtgctgg ccgatgcacg agtgagttgg aggctcacgc
     5821 gttccggcga attgagtatt tcgctgcaaa atcttacgaa ccggcaggct gtcgagagct
     5881 attccgaacc gccgtacgcg gcaattccat tacgcaggac tttcgtcgtc aaatggacac
     5941 acaagttctt cgcagactga agataggcat aaaccgggtg ccgggctctc gtctgacagc
     6001 gtatattgcc atcgcctcca tgctggccct ttccgcgctc aaagcggccg agccggaaga
     6061 cgaactcaag gcagcagttg tgctgagctt catccgccac gcggagtgga agccctctcg
     6121 cgctccgggc atcactatca gggtaggcgt taccgggcgc cccggttttc gagaagctct
     6181 caagcgaact ctggaaggca aaacggttgg aaacagcctg gttcgggtaa tagatcttga
     6241 aacggacatg gataccccct gctgccatat cgtgtacttc gcaaccacaa agaactccga
     6301 agccaagcac cgtctttcga tgccgtgggc agcacgtgcg atcacgatcg gcgaggcgga
     6361 ccagttcatt gaaaacggcg gcgccgttgc cgttcacctt atggacgggc gtatccggtt
     6421 cgaagtgaac ttgagggaag ttcaacgggt gggcgcctcc atcagctcca aattacttcg
     6481 attcgggcgc atccgggtcc ccgtaggagg cggcggatga gaaggtcatc ctcactccgt
     6541 tcgaagttca tggcattaat gctcggcggc agcgtcgccg cggcggtcct tgccgccggt
     6601 ggattttcat ggctggattt aaaccgtttt tgggcgcgaa caaccgacga gatctcagcc
     6661 atcacgagta tcgttgcgga ccaggtgggc ccagccatca cgcttcgaga cgcgaaggcg
     6721 gcacgcgaaa ttctcggatc gctccgggct gttggtatca tccgccatgc cgccatctat
     6781 gacaagcacg accgttgctt cgcggtgttt cagcgcgaca gttcttccat ctgcccgcct
     6841 agaccacaag acggcaccac atatggcccc gggaccatga tcgtttcgcg cggcgttttc
     6901 tcggatggcg aacgtctcgg taccctggcg ctttccgcca cggttccgtc gctttccaaa
     6961 gtgttgcgcc aatatctcga tggctctttg atcgttcttc tgctgagtct gatggcggca
     7021 acggttcttt ccgtgacgtt acaggctcgg attctgacac cggtattgaa gatcgcggaa
     7081 atcgccggac gcatcgcccg cacacatgga tatggagagc gtatcaatgt cgcttcgcgc
     7141 gatgaactgg gtgtgctggc cagttcgttg aatgccatgc tcgatgagat tcaacatcgc
     7201 gatgcggagc tgcaacggca ccgggaccgg ttggaggagg aagtagccga acgcagccgc
     7261 gtgaatatcg agttacgcca ggcaaagcag agagccgagg acgccgcccg gttgaagagc
     7321 gagttcatcg cgaatacaag ccacgagatc cgcacaccga tgaacggcgt tatcggcatg
     7381 atcggcctgg cgctcgagac ttccgcggac ccggaacagc gcgaacacct ggagaacgca
     7441 ctcaatgctg cgaagtcgct catcgttgtg ttgaacgata tcctcgattt ttcaaaaatc
     7501 gaggctggaa aaatgacgat cgagagcatc ccattccaac tccggaaagt gctcagtgag
     7561 tcactccgcc tgttcgagtg cactctgcgt gagaaaaacc tcggcgtcca attcaccgtc
     7621 ggccaggagt gtcccgagtg ggtgcgggga gatccgacgc gccttcgaca gatcatcgtg
     7681 aatctggtgg gaaactcggt gaagttcacc ccggccggat ccatccggct ggcagtgaca
     7741 acgctgcctt cggaccggat tcgtttcgaa gtgcaagata cgggtgtggg gattgccgcg
     7801 gaaaagatcg gtttgatctt cgatgcattt actcaggctg atggttctca cacacgccaa
     7861 tacggtggca caggcctggg cctggctatc acaaaacgtc tggtgagcct gatgggcggc
     7921 accattcgtg cgcgcagcga attgggatac ggcagttgtt tcaccttcga actctctttg
     7981 cccgaagcct atgacaatcg cgaggacgaa gagctgggaa ccacctgcaa gctcactccc
     8041 ggtctcgaac acttacgggt gctggtcgcc gaggacaaca ttattaacca aaaagttatc
     8101 tgctctatgc tcgagcgtct cgggtggtac gtggacctgg cggagaatgg caagcaagcg
     8161 gttgccttgt tccgggaccg tgagttcgac ttgctgctga tggatattca gatgccggaa
     8221 atggatggac tggaagcagc tcgccggata cgagaagagg agcggggacg gaatgggaaa
     8281 cgcacgccca tgatcgccct gacagctcat acgggccaat cgcaacagga ggaatgtatg
     8341 agtgcgggaa tggatgcagt cgtcacaaag ccgtttgaac ttcagtcgct tctcgagtcc
     8401 ctgaatgctg ttcgcgccgc ctcggcggtt acctctgaac aggcttgagt tccaccttgc
     8461 ggatcagaag ctcggcgcct tccgattgga tctggatctt cccttccctg tgcgacgact
     8521 tcgttccacg gttcactatc gccccattca ggatgttggt cagcgtgccg ccgtccgcaa
     8581 ccacttcctg cctgttccac tctcccacag gtttctccac atcgttcggc ccgcggaaac
     8641 cgatcctgtc cacccagtcc ggatcgcgac cgaaccagtt gaaacggccc cggtcgcggg
     8701 tccgcggttc gccaccctcc tgccagtaga gttcaccgcc ctttccttcg cggctttcca
     8761 ctgtcaatga gggacggttc ttgcccccca ccagaatgaa gtcccctgtt ccgccttcaa
     8821 taatttgtgc ctcaatcgat tcgagccaga ttcctccagc ggcgccgtcc tccccaaccg
     8881 catgcaccag gattccgctg tcgcgcgccc tgtcctttcg ggaaccccag gtcggcccac
     8941 cccatctcca ttcgacaacg aggtggtagt ctttccagga ctgccgggtt acgatgccgc
     9001 cccactcctc cccggaaatc cggagcagcc cgtctttcac ggagaagacg cgtttcggat
     9061 cctcgtaccg gttctcgcgc agccaggtgt accagttgct gatatcatgg ccattgaaca
     9121 actgaacagc ttctttcgga cggatcgccg caacgggttc ggcgaacagc ggaacgaggc
     9181 caatgcacag gcagagaagc ggtttcattc gtccattgat accacggcta acggcctatg
     9241 cggaagacgg gttatctact cgtgttccaa cctggaacag gcattcgaaa gtggcacgtt
     9301 cctgcgcggg atgacaactt tctgccaccc aactggggct gtgcaaccca ttaaaatcag
     9361 catactgacg gttgtcttgg acggcatgaa acatgcaatt gtcccaggcg tgatactagt
     9421 tcaccggctt gccggcagcc tgttgctatc tttttgcctg gtctcgccct cccacacaga
     9481 gacccgcggc accgaaccgg agggtgctgc ccggaaatgg accactatca aaaagtcgcc
     9541 tcgaaaggcg gctgagaccg agggcaagtg ggaaatcttc aaagacatca cagtcgatgc
     9601 gggcaagagc gtagtgctcg attccagcat cgattattct tctctcgaca aggtggctgt
     9661 tgcggtgcgc tcggcgtccg attcgctatc cgatctcgaa agcctcaccc tacagacgtt
     9721 ctggtctgta ccggatgccg attatttcag cgcggcggag atcaagttcg ggaaaacttt
     9781 tccgttcaca aacagcggca gcgttttgtt tggcgtctac ggctcggaat tccgattaat
     9841 tctgagaaat gaaggagcct tgagcttaaa gatcgcgcag ctctttatct tctgccgatc
     9901 gcaatagccg cgcttactct gttttcagcg ttgcgtactt tgcgatgatt ttcttcaggc
     9961 cggcgcgaaa actgaccgtc agtttggtgt catcaccctc accctccagc cgcacaacct
    10021 ggccgcgccc gtacgtgggg tgataaacga tcgatccttt acgaaatccc tgagccagac
    10081 cggcaccgga ccggtcggcc gccggttttg cggtttgcgc cgttcttgcg ggaccgggag
    10141 gagccgttgt ccggttcaag ggaggtggcg tgatgccccg ctccgtaaag aattgtgcga
    10201 tgttgtccag cgaattataa gtcttgccgg tgtatgtatt gcgcttggcg ctctgacgga
    10261 cctcgtgctg ctctacgaag agatccacct ggggagtttc gcgtctgctg cggtgcagag
    10321 cctgcacaaa ctcgcgagga atttcggcca gaaaccgcga agggatgcag ggctccggct
    10381 gtccgccgcc ccagcgccgg cgggaacgcg cccacgttag aaatagccgt ttctccgccc
    10441 gtgtcatgcc gacataacac agccggcgct cttcttcgag agccgcctcg ctggtcaccg
    10501 accggctgtg gggaaacaat ccttcttcca cacccgccat gaacaccacc ggaaattcca
    10561 ggcctttcgc gttgtgtatg gtcagcaggg agaccgccgc gtgttcgtcc agtccgtcag
    10621 catcagcgac cagcgcggcg tggtccagga actccgttac cgtctcacca cgttcggccg
    10681 cgtcaacggc ggcattcagc agttcgttta cgttggcgat gcgcgactcg gcttccggag
    10741 aggagtcggt ctccagcatc ttcatgtagc ccgtccggtc gatgaccgct ttcaacaggt
    10801 ccccggctcc cagggcagcc atctccgtca actcctccaa taaacgccgg aacgaagaca
    10861 gcgcggcctc ggcgcgagta gacaactgct tttcttcgat catgcgatcc acggccgacc
    10921 acaaactcag attgtgctct gtggcaaatc gttcgagttg ctcgatggtg ctccggccga
    10981 tgccgcgcgc aggggtattg ataattcgca gcagggcgat tgaatcgtgc gggcttacac
    11041 tggcgcggag gtacgccagc aggtccttga cctccgcgcg ctgataaaag ctgaagccgc
    11101 ctataaccag gtatttccgg ccgtagcgcc gcaatgcctc ttcaatttgc cgggactgcg
    11161 cattggtgcg atacaggacg gcgatgcgtt cggcgggatt acgcgaaaac atctgttcga
    11221 tggtatcggc gataaacaac gcctcatttt cgccgtccca tgcctcgtag aagccgatgg
    11281 gctctcccgc gccggattgc gtccagagcc actttccttt tcgcgccgtg ttattcgcca
    11341 ccagcgcgct ggccgattcg agaatgttct tcgtggaccg gtagttttgt tccagccgga
    11401 ttactttcgc gttgggaaag tcacgctcaa agtccagaat gtttcgtatg tcggcgccgc
    11461 gccagccgta tatcgactgg tcctcgtccc cgacgacgca aatattctga tgggcctggg
    11521 tgagcagccg catcaggtca tattggctgc gattcgtatc ctggtactcg tcgatcatca
    11581 cgaatccgaa tcgccggttg taggcggccc tcgtcggctc gtcaatgcgc agcagccgca
    11641 cggcttccag cagcaggtcg tcaaaatcca gcgcatttgc ctgttggagc cgctcttcat
    11701 acatctcgaa cagcgaggcg agccgcgagc ttttgggatc cgtcgacgcg ctgtaaagat
    11761 ccgcgggtgt ctggttctgg cttttcgcgt gggaaatacg ggatagcacc gagcggtagg
    11821 gcatgaactt ttcgtcgagc cccgcgtggc ggaagatcgc cttgatcagc gcaagctggt
    11881 cgtcttcgtc ataaatcgtg aaattacgcg taaagttgga acgaaccgct gccagcgggt
    11941 cgccgtcacg ccgcaacaaa cggacgcaga aggaatgaaa agtggaaaca acgggtccgg
    12001 aacggggatc ctgcgatccg gcaaggttca gaacgcggtc gcgcatctcg cccgccgctt
    12061 tgttcgtaaa tgtgacggca agaacctggt atccgggaat accacgggtt cggatgaggt
    12121 gcgcaattcg gtgggtaatg acacgtgtct tgccgctacc cgcaccggca aggatcagaa
    12181 gcggaccttc gacgtgttcc accgcctcgc gctgttgggg attcagcgca gtcagga
//
LOCUS       DASTMK010000543        21149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_443673, whole genome shotgun sequence.
ACCESSION   DASTMK010000543 DASTMK010000000
VERSION     DASTMK010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21149
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443673"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..958
                     /locus_tag="VFQ79_22450"
     CDS             185..958
                     /locus_tag="VFQ79_22450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0142500.1"
                     /translation="MSYGSQHVLNGVDLDVHRGEILVLLGGSGSGKSTLLRQVLGLKK
                     PDAGSIIIDGVDITACSGDELQQVRRRIGVAFQAAALFNSLSLRDNVALPLRELTRLA
                     EPTIQLMVWMKLRAVGLADAGGLYPPELSGGMRKRAAIARAIALDPEILVFDEPSAGL
                     DPIVSAGLDELILFLKNALHMTMLVVTHELQSAFRIADRLAMLYKGSLIAVDTKEEFS
                     RNVHPRIRQFLDRQPDSESGGMTDAFIESYLWGAAKHEH"
     gene            1005..1913
                     /locus_tag="VFQ79_22455"
     CDS             1005..1913
                     /locus_tag="VFQ79_22455"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0142501.1"
                     /translation="MAVYFVRTTQTVKGQVPYSTYLRYAGGLAPGATVLFGGIKVGQI
                     TAVDPWSNDPTLIEIRFEVKTGTPINENSTARVGSVSVMSSPALLITTGSNDARRLTA
                     GQAVRSEEAVSLEEITRRIGAVADSANKLVTQLQEDIPAITGQADTFLANLNEISGPK
                     NRRQIEQALAELNALLARESPKIDRITDQISILVERADSAVGSIEPLVANVDRTVMNV
                     NGTVDALREPLRKDLAELEGTLQEARMLLASIQNVVRTNDSSIGEMMQNLRSTSENLQ
                     VLTESLKQRPFSLIRITQPADRKVPQ"
     gene            1910..2527
                     /locus_tag="VFQ79_22460"
     CDS             1910..2527
                     /locus_tag="VFQ79_22460"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEU0142502.1"
                     /translation="MKRGGSPHLLASALLGLLLLSACGKVRYPTNYILNFPRPASPVV
                     APDAAFGPVVINEFRCLEYLCQGRIVYRPRPEEIGFYEYHRWAMDPRQSITQLMADTL
                     RARSIFKHAAMQGRGIEADYILNGGIERLEEVDEGRDVRVECAISAQLIDTRTGAIVW
                     SGAASQTIPVGKRDVAGVVVALTTAAQTVVDRLVKSMTDQLMSTR"
     gene            complement(2547..4529)
                     /gene="ftsH"
                     /locus_tag="VFQ79_22465"
     CDS             complement(2547..4529)
                     /gene="ftsH"
                     /locus_tag="VFQ79_22465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU0142503.1"
                     /translation="MTDQNAKPQQDLKHAGERDDQRPPRPWRTEGLPAKEKPPDKRRR
                     DWFGMAAWFLGYLVFFGLLTMQDRMDRPQAVSYTEFKTQVANKNVAELFARGDTIQGE
                     LRKAVPLPGQQDKTDQQVRTYQKFTTERPTFAADDLLSELAKSNATVRATPLVKQRGA
                     LTNLLISLAPFLLLILFYSWIFKRQKSAMGGGLFGGGARKPVDPEAVRVNFNDVAGID
                     EVKAEISEVVDFLRNPEKYQRLGARAPKGVLLAGAPGTGKTLLARATAGEADVPFFSA
                     SASEFIEMIVGVGASRVRELFSEARKVAPSIIFIDEIDTIGRTRAGARAVGGHDEREQ
                     TLNQILTEMDGFSGHEGVVVLAATNRPDVLDPALLRPGRFDRTIMVHSPDHKGRADIL
                     RVHTRKVPLAEDVDLDRVAASTPGMTGADLANLVNEAALLAARREEDAVSQRDLTDAL
                     EKVQLGTARNVVIPEEERRRTAYHEAGHALLGMLQPGADPVRKVSIIPRGRALGVTLS
                     TPESDRYGYDVNYLRGRIIGALGGMAAEQEVYDVITTGAESDLETVTRIARSMVGRWG
                     MSDRIGTVSVLPAEGDPRMAGTSDGMLDAVDQEVHRITDECYVEARRLLRENRDKLDS
                     IVEQLLIHETLDEPDVYAAAGIARPAAALKPAPALL"
     gene            complement(4620..6029)
                     /locus_tag="VFQ79_22470"
     CDS             complement(4620..6029)
                     /locus_tag="VFQ79_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0142504.1"
                     /translation="MKPVFFTLAALTALTGCAVGPNYKRPQVAAPTAYRGAVAEQTAS
                     GSSASLADTKWENLFPDPALNDIVSAALRQNFDLRVAAERVEQARAQLGINRANQFPF
                     LDIGAGFSSVRNSSVGANVFVPRGTDLSASFTEAGAQLSWELDVWGRLRRLTESARAQ
                     YLATEEGRRAITVSLISEVMSTYFLLLEQDLELAISRKTRDVAENSLKLVRLRRDRGA
                     ASGLDVRQAEQLLYTATAQIAAVERNIAQTENALSLLQGNAPSAQVRGRRLEQIQVPD
                     QLPAGIPSSLLERRPDIRAAEQNLIAANAQIGAAKALYFPQISLTAFAGGQSRSLLDI
                     VTAPARVYSFAPSALLPIFRAGQIRSQVRLTEAQQRELLVRYQQTIFTGFREVSDALA
                     EFDGTRKQRAEQTQLVRALTETVELSEARYKGGLDSYLQVLDAQRNLFAGELTLAQLQ
                     LRERSAVVQLYRALGGGWQ"
     gene            complement(6026..9175)
                     /locus_tag="VFQ79_22475"
     CDS             complement(6026..9175)
                     /locus_tag="VFQ79_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU0142505.1"
                     /translation="MARLFINRPVFAIVLAIVILILGGVSIPFLPIATYPEVVPPVVQ
                     ITAVYLGGNGEDLEKTVAQPIEEQLVGLDGMLYYQSISSNDGQMNIRVTFELGTDPDI
                     ATVQTQNKVNAALPRLPEEVQRQGVKVQKVSSQLLLVINLISNDKAYDSQFLANYAQI
                     NLLNQLGSIPGVGESRLASQETYSMRVWLDPDRMSKLGLTATDVAAAIQAQNRQNPAG
                     AVGQAPTPQGTDLQFAVRAPGRLSDISQFEDIVVRAQPDASLLRVRDIGRVELGAQSY
                     AGFSRLDGQPSGAVILYLSPGANAVETADKVVAFMDEAQKAFPPGIEYRVPYDSTMFV
                     RAAITDVAKTLMEAVALVVLVVFIFLQSWRATLIPLLTIPVSIIGTFAVFPLLGFSIN
                     ITSMFGLVLAIGIVVDDAIVVVEAVQRHIDDGMAPREATIRAMNEVSAPVVAIAFILA
                     AVFIPVAFIGGISGQIYKQFALTIAVSVLISAFSALSLSPALSAMILRPHRKSGGLAG
                     KFFGAFNRAFNRTNKRYRRGVIHLIRRPALVIVFLLFVAFSSGWLFHTLPTGFLPDED
                     QGAFFSSIRLPDGASTERTDAAARKVEKLLAGIPGVEGVMTIGGLDIVTQTSSSNVAT
                     VIGRLKPWQDRKTRETQLEAVLAEAYARFATVPESLNLAFGLPPILGLSTTNGFQFML
                     EDRAGTDIQTLSKTADALVAAARQRPELANVISTFRGSVPTYNVEMNTDKLQTLGIPV
                     TDAYNALQTFLGGLYVNDFNVFGRTWQVRMQAEPRFRDEPTDIDRFFVRTRDGEMVPM
                     GTLTNLQTTGGPEAVYRYNRYRSIYIMGNAASGYSSGQAADAMQEVADATLPPGFGYE
                     WTGTTFQEKQAQGNEAAIFAFSSILVFLSLAALYESWSIPFAVLFAIPLGILGALTSI
                     FLRSYPYDIYTQIGIVTLIGLSAKNAILIVEFARERYEHGHSIGKAAVEAAQLRLRPI
                     LMTSFAFILGVLPLVIATGASSGARRALGTVVFGGMLAATLLPIFVVPVLFVLVVKFV
                     DQKKKREAIGPAPVPEAAQ"
     gene            complement(9179..10519)
                     /locus_tag="VFQ79_22480"
     CDS             complement(9179..10519)
                     /locus_tag="VFQ79_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0142506.1"
                     /translation="MFPSLPRTLLSHRRSRSVLFALTITVTSFGLAGCRSVSAQKNSG
                     EAAHPPLVEVVELAPTTAELLAEYPAQTYARNMVEVRGRVDGYVDKWLFRPGQFVKQG
                     QALYVLDLRPYQAQVQQAQGALRQSEADLTFARQQVSLLEAEANLASAEANLVKARQD
                     YERLQPLVQQDAAAQQDLDAAVANLRAAEASVRAHKANVEQTHLTTGTQIQSTEGKVE
                     AQRGALETASLNLQYATIRAPISGIVGDSVVPVGGLVTANSPQPLTTIVPLDTIWVRF
                     KVSESEHLALTRRYTGSKDEGPALRMILADNTEFPHIGRIRNALNQVDPKTGTLELQA
                     EFPNPGHTLLPGQFGRIQYIAERREGVLVIPQRAVQQNQNLQLAYIVGPGNKVEARSI
                     KTGQRVGDSLLVEEGLKAGDRVIVEGLMTIRPGATVTPKPWTAKSPAASPGKVG"
     gene            10636..11277
                     /locus_tag="VFQ79_22485"
     CDS             10636..11277
                     /locus_tag="VFQ79_22485"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0142507.1"
                     /translation="MVTSPGIKGRASRADAAKSRRQAILKAARTVFARQGYANTLIDD
                     VAAAAGIAKGTVYLYFASKEQIYLEALLERAREVEARSRARILEAATWQEKIRAYMET
                     RVEYLNAHQDFFRIYATEFRNMCMQGKAIGAELQDLVRQGEQQVAQVLAVAAAKGEIR
                     PLDPDLGAAMISDLMRGLVDRTLLAFRRTPVAEDLEFTLDCVYRALAKEANPA"
     gene            11472..12134
                     /locus_tag="VFQ79_22490"
     CDS             11472..12134
                     /locus_tag="VFQ79_22490"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEU0142508.1"
                     /translation="MATKKPRNGNGKSGEKLTVAGAQKLSTEELALYIKSALIKEALE
                     DPAFRKLALKDPASAIQKSRLTGGVEKALPAGFTFRVVEETQQLLYLVIPHKMPERLD
                     PKNPKHILLKKAVSDPQYLQKLTADPRSVLQSEFLVDVPPGFQIRVIKETPLESVAVL
                     PADLRPEQITAIPKELIEYQASGWCGKTLKTIIENLCPKPPGETEFESPFCTPDCGVQ
                     PL"
     gene            12168..14336
                     /locus_tag="VFQ79_22495"
     CDS             12168..14336
                     /locus_tag="VFQ79_22495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142509.1"
                     /translation="MSRNRRSLAESLEVVARYVPRTLIRPRQMQAIRELAGLLPPIAV
                     AGFECRLAEVGGRVDFAVFLPSSDSCFSVVAGMNPSHELAPELLEDPTWQHIRTFCRS
                     VHEPGGPLHGVVDDVWLEFDLPSNGNPTLTPALFFGISRAVTSSPGNSLEIVESTLKS
                     LGAPPLAGITQSEFLRAASCLPVDSVIFQIGIPLAYRASALRLCISGLPPGGIAPYLR
                     KIEWRDTPLGLENTITDLAGIADELFLDIDIGEISTRKIGIEAHFRGPKRPIIDRRWE
                     DLLSWLVREGLCAPEVERDIRRWTGTTYFGEGGAPKPKELLALTKLLGGRATLCFLRG
                     LHHVKISYEPRRGFEAKGYIGFKRHWIETMPANLPALKRSGRPPFESSVRDTLAAGVE
                     FLAGSRDPNGWWKDFELAAGASDTWVTAYVGWALAGVDEPQSWEMAHAAWCLLKQSLH
                     PHGGWGYNLKVSPDADSTAWAILLGESVGDSRSLALRTAKNFLRSHLRTSGGVATYAE
                     ESPVRAEMRFPAEVSYAGWTTAHPCVTALAANIQSFRARLIPFLLRSQRKDGNWPAYW
                     WMDPEYATALAVLALNGIEGLTPEPASRLRKAVRWIELRLAEHLSLMPDASAPQVFAI
                     AWCLKALAACAVSSGQAALIQQAMNRLIDAQLPDGSWTASARLRVPPTDCMNPNRIHS
                     WYPGGKAIRGISLDQQRTFTTATAVSAISAGAERLAELTH"
     gene            14333..17548
                     /locus_tag="VFQ79_22500"
     CDS             14333..17548
                     /locus_tag="VFQ79_22500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="HEU0142510.1"
                     /translation="MTFHDLAVLVAASSSLYERIQKGFQPVSNLNPDSSTSQELARWK
                     QIYRDKGPGAFKRRLALDGWTESFVQPYLGAVRLPHESDLPAWALTLRGCVNRMLESK
                     PGDFLASGDESPQPRLFEHLFTPMVQFASLELRRRAGDKSRLLSTTALAGAELSLLDS
                     LVQVSIGTLALEFQAFRSAREDTIFRILRRMKDQRSNELYRAFLVQMHAGGLLNLLSA
                     YPVLGRFWGLLAELWIENLTEFLTRLDTDLPTLSRRLKVSSRRPAVANLRMNISDRHH
                     GGRTVMLVVFSGERRVIYKPKGLGLEKAFFQLLSWLNREGSPVQLRGIEVIDRETYGW
                     VEFADHRSCVNRDEVASYYRKAGGLLCIVHLLEGTDCHLENIVAARADPVLVDAETLL
                     HHRVVRDRSGLESGFEAQDECWDSVFRTGMLPSWILEADGTPFDISGLGGFGEQASSQ
                     RVPHWDNINTDELQLRSLPGRRRPARNVPMLGETAQSPLAFESELIAGFSEMYRFCDR
                     HKALLTARKSPVLPLGSHWSRYVARDTTVYCELRRGLLQPSALTSGIAQTLHLELLAA
                     AHMANTARPEPWKIVRSEREALLQFDIPYFRARACSRDLELPDGKLIAGFFERASIER
                     VLRKIAGTSPEDLRKQVRFIRASFFSRSVRTPHQRNSVSTLRRKDVPGTSAFRQAQFI
                     AEAERIAEELRKDSARVNGTGAAWIGLDYRPESKRYHYQPVGNGLYSGRCGIGLFLAA
                     LWATTGETRWRELALGAVDGARRRITEKGWQVADASIAYAICRIGTFLADEDLLRDAT
                     KAAGRIGRGAIASDENLDVLFGTAGALLSFLALHKWCGDEAALAKAVACGRRLLLARV
                     RTGTGHRAWQSRELPALTGFSHGAAGIAYALLKLFETTGQESFREAALEAIAFERAVF
                     DAEAGNWPDFRSGEGRQGTRGFMTAWCNGATGIGLSRLGARLAIADGPVAQEIEAALR
                     ATRGYRYDDVDHLCCGSFGRIELLLTAGCQLHRPRLVAEARRWASVRIQQSERNGGYR
                     VMHRLPRWVRNPGFYQGISGIGYQLLRLARPKDLPILLLWE"
     gene            complement(17560..19737)
                     /locus_tag="VFQ79_22505"
     CDS             complement(17560..19737)
                     /locus_tag="VFQ79_22505"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU0142511.1"
                     /translation="MRSLVFFAVFFFLTSISTLAERITVSLDGEWQIEDSVDADAVPR
                     NWTHRVPVPGLANLAQPAFADVDRFDGKEVLWNRIRRKVIPASTSLEGQGISRQNRNY
                     FWYAKSFRVPARKQVALLRIGKAQFGTAVWLNGKKLGEYAGCFSASYFDLTQAMDWAG
                     ENRLIVRIGAHPAVLPESYPAGSDFEKNLWTPGIYDSVSVLLMDNPVIETIQVAPAIT
                     PPRITVQTRLKNYGAAPATTTLRHQVKAWKGGQRASQSTAEKVSLKPGEEKTVTETIP
                     IPGARLWSPEDPFLYVLDSSTGGDTASTRFGVREFRFDTPTKRAYLNGKVYFLRGSNI
                     TLHRFFEDPDAGSLTWDETWVRKLLSEIPKKMNWNMFRFCIGPVPDKWLELADETGLL
                     IQNEFFIWTGHVSGNYKRQWDADELIRQYKDWMRDNWNHASVVIWDANNESYDPVFGE
                     KVIPAVRDLDLSRRPWENSYNPPAGPDDPVEDHPYLFVSGRTGNPFQVTSLESMSGSG
                     RNTVTPSGHAMVLNEYGWIWLLRDGTPTVLTERVYEHLVGKTATGKERLALNGYLLGG
                     LTEFWRAHRNYAGVLHFVYLTSCYEGAYTCDHFSDVKSLTLDPTFADYAGEAFKPLGV
                     YLNFWQPNLKAGAERRFTVMMVNDSSAPASGNLVLTLDNEGGEAVARVERKYSIQPLG
                     QETLLLDMKIPDVAGSFLLKAAAHAGDGSGPTLSRRRVSLQKE"
     gene            complement(19783..>21149)
                     /locus_tag="VFQ79_22510"
     CDS             complement(19783..>21149)
                     /locus_tag="VFQ79_22510"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0142512.1"
                     /translation="GAPMQELGQRFWKLLDGKTVFYSPKGPRNTTETTTAGMKGRMTV
                     NQGLFHDRKIPAFLMELMVEPSPRLSREPGVKDRLQFGEVLVRVMAASVAAPAPTSAA
                     QGTPADIVERNDAYGRELEQHLQNYLIEQYPERASRAWKRDYSSQQAFLKSVGPNRDR
                     YKKFLSPPELRVTGPVQRRPRPLRELPQAEWISVPLGSLKAEGLLVFPKNTSGRVPLV
                     IAQHGIGSFPERVFGVDDPGNAYHDYGHALVEQGFAVLAPANLYSVEKRNRIERLARL
                     ADTTLPGIEFRRMQALLDYALKDPRVDSERVGFWGISLGGMAGMFWTPLEPRIKCSII
                     TAWFNHRRNKMALPDERYSCFLDTKEEHAFFNGWLTEFTDSDVASLICPRPLLVQHGK
                     KDRIAHWPQVVEEFEVAKEHYRKLGLEDRIGLNVHEGGHEIVLETGIPFLKRWLNVPA
                     SAAK"
BASE COUNT         4239 a         6224 c         6413 g         4273 t
ORIGIN      
        1 gaggaggtcc ggaggctgta ggcgcggcgg cgacgtcggc ggtggtgaaa tccacgttcc
       61 tggtaatcct cgccgacttg atcgctacgg gcatcttcta cctgatggga tggagttccg
      121 ccggatgata tcccctaata gcgggaatgc cgccgagcct gtcatttcga taaggaattt
      181 gtccgtgtct tacggatccc agcatgtgct gaatggagtc gatctggacg tccaccgcgg
      241 cgagattctg gttctgctcg gaggaagcgg ctcgggaaag agcacattgc tgaggcaggt
      301 tctcgggctt aagaagcctg acgcgggcag catcataatc gacggggtcg acatcacggc
      361 gtgttccggg gacgaactgc aacaggtccg ccgccgcatc ggtgtcgcgt tccaggccgc
      421 cgcgctcttt aattccttat cgcttcgaga caatgtggct ttaccgctca gagaactgac
      481 acggctagcc gaaccgacaa ttcaattgat ggtatggatg aagttgaggg cggtgggact
      541 tgccgatgcc ggcgggctct atcctcccga gctttcggga ggaatgagga agcgggccgc
      601 catcgctaga gcgatcgcac tggatccgga gatcctggta ttcgatgagc cttcggccgg
      661 gctcgatccc attgtttcgg cgggcttgga cgaactgatt ctgttcctga agaacgccct
      721 gcatatgacg atgctcgtgg tgactcatga actgcaaagc gcgttccgca tcgccgaccg
      781 tctggcaatg ctttacaagg gatctctgat cgcggtcgat acaaaggagg agttctcgcg
      841 caacgttcat ccccggattc gacagttctt ggacaggcag ccggacagcg aatcgggagg
      901 aatgacggac gcttttatag agtcctactt gtggggagca gccaaacatg agcactgagg
      961 cgaaggtagg cgtattcgtc attctcagca tctttgttct cggagtggcg gtgtacttcg
     1021 tgcgaacgac gcaaaccgta aaagggcagg tgccgtacag tacttatctg cgttatgcgg
     1081 gtggacttgc tccaggtgct actgtattgt tcggcggcat caaggtcggc cagatcaccg
     1141 ccgtcgaccc gtggtccaac gatccgactt tgatcgagat ccgcttcgag gtgaagacag
     1201 gcacgccgat aaacgagaat tcaacggcgc gggttggctc ggtaagcgta atgagcagtc
     1261 cggcactatt gattacaacg ggaagcaatg acgcgcggcg tctgaccgcg ggtcaggcag
     1321 tacgttctga ggaagccgtc agcctggaag aaatcacaag gcgcattggc gccgtggcgg
     1381 actctgccaa taaacttgtc acccaactgc aagaagacat cccggcgatc acaggccagg
     1441 cagatacgtt cttggcgaat ctgaatgaga tcagcggtcc gaagaaccga agacagatcg
     1501 agcaggccct cgcggagctg aatgctttgc ttgccaggga atcgccgaaa atcgaccgga
     1561 tcacggatca gatctctatc ttggtggagc gcgcagattc ggctgtcggc tccatcgagc
     1621 cgctcgtcgc caacgtggac cgtaccgtga tgaatgtaaa cggtacggtg gacgcactca
     1681 gggaaccgct gaggaaagac cttgccgaat tggagggcac attacaggag gcgcgcatgc
     1741 ttctggccag catccagaat gtagttcgca caaatgacag cagcatcggc gagatgatgc
     1801 agaacttgcg ctcgacctcg gaaaaccttc aagtattgac ggaatctctc aagcaacgtc
     1861 ccttcagtct cattcggatt acacagccgg ccgataggaa ggtaccccaa tgaaacgcgg
     1921 aggcagccct catctgcttg cctcggctct tctgggatta ctgttgctga gtgcgtgtgg
     1981 caaggtgcgc tatcccacaa actatatcct gaatttcccg cggcccgctt cgccggttgt
     2041 ggcgccggat gcggctttcg gtccagtcgt tatcaatgag tttcggtgtc tggagtatct
     2101 ttgccagggc cgcatcgtgt accgcccccg tccggaagag atcggatttt acgaatacca
     2161 ccgctgggcg atggaccccc gccaatccat tactcaattg atggcggaca ctctccgcgc
     2221 acgatcgatt ttcaagcacg ccgcaatgca agggcgcggt atcgaagcgg attatatatt
     2281 gaacggcggc atagagcggt tggaggaagt agacgaaggc cgcgacgtcc gcgttgaatg
     2341 tgcgatctcc gcgcaactga tcgacaccag aacaggagcg attgtctgga gcggagcggc
     2401 gtcgcaaacc ataccggtcg ggaagcggga tgttgcgggt gttgtcgtcg ccctcacaac
     2461 agcggcacaa acggttgtcg accgtctggt caaatcaatg accgatcagc tcatgtccac
     2521 gcgctaaccg cgcccgaagc aagtttctac aggagcgcgg gcgccggttt cagcgccgct
     2581 gccggtcgcg caatccccgc ggcggcgtac acatccggct catcgagagt ttcgtggatc
     2641 agcagttgtt caacgatgct gtcgagcttg tcccgattct cccgcagcaa gcgcctcgct
     2701 tcgacgtagc attcatcggt gatacggtgg acctcctgat cgacggcgtc gagcatgccg
     2761 tcagaggtgc ccgccatgcg ggggtcgcct tccgccggca gaacggatac cgtgccaatg
     2821 cgatccgaca tgccccaccg gcccaccatc gagcgcgcta ttcgggtaac cgtctccaga
     2881 tcgctctccg cgccggtggt gattacatcg tacacttcct gttcggctgc cataccgccc
     2941 agcgccccga tgatccggcc acgcaagtag ttgacgtcat atccgtagcg gtccgactcc
     3001 ggagtggaca gggttacgcc cagtgcgcgg ccgcgcggaa tgatcgacac ctttcggaca
     3061 ggatcggctc ccggctgaag catgccgagc agagcgtggc cggcttcatg ataggcggtg
     3121 cggcggcgtt cttcctcagg tatcactaca ttgcgcgccg tgccgagttg taccttttcg
     3181 agcgcgtccg tcaggtcgcg ctgagagacg gcgtcttcct cgcggcgggc ggccagaagc
     3241 gccgcttcgt taaccaggtt ggcaagatcg gcgcccgtca tgccgggagt tgaggcggct
     3301 acccggtcca gatccacatc ctccgcgagc ggaaccttgc gggtgtgaac gcgcaagata
     3361 tcagcgcggc ccttgtggtc gggcgaatga accatgatag tgcgatcgaa ccggccgggg
     3421 cgaaggaggg cagggtcgag aacgtctgga cggttcgtgg cagccagaac aaccacgccc
     3481 tcgtgtccgg agaagccgtc catttccgtc aggatctggt tcagggtctg ttcacgttca
     3541 tcgtgacctc caacggcgcg cgcgcccgcg cgggtgcggc cgatggtgtc gatctcgtca
     3601 atgaagatga tggagggcgc gaccttgcgg gcttcggaaa aaagctcgcg cacccggctg
     3661 gcgcccacgc cgacgatcat ctcgatgaac tccgaagcgc tagcgctgaa aaagggtaca
     3721 tcagcctccc ccgcagtggc gcgggccagt agtgtcttgc cggtgccggg cgcgcccgcg
     3781 agtaacacgc ccttaggagc acgggcgccc aaacgctggt acttctcggg attccggaga
     3841 aagtcgacca cttcgctgat ctcggccttc acctcgtcaa tcccggcaac atcgttgaaa
     3901 ttgacgcgaa cggcctcggg atcgacgggc ttgcgcgcgc cgccgccaaa gagcccgccg
     3961 cccattgccg acttctgccg cttaaagatc caagagtaga aaaggatcag caacaggaac
     4021 ggcgcgagcg agatcagcag attcgttaag gcgccgcgtt gcttaaccaa cggcgttgcc
     4081 cgcaccgtcg cgttactttt cgccagctca cttaacagat cgtcggctgc gaaggttggc
     4141 cgctcggtcg tgaacttttg gtatgtgcgg acctgttgat cggttttatc ttgttggccg
     4201 ggtaacggca ccgcttttct aagctctccc tgtatcgtgt cgccgcgagc aaatagctcc
     4261 gcgacatttt tgtttgcaac ctgtgttttg aactcggtgt aagaaaccgc ctggggccgg
     4321 tccatccgat cttgcattgt cagcaggccg aagaacacaa gatatcccag gaaccatgcc
     4381 gccatcccga accagtcgcg acggcgctta tccggcggct tttccttcgc gggaagccct
     4441 tccgtacgcc agggtcttgg cggacgctgg tcgtcccgct cgccggcatg cttcaggtcc
     4501 tgctgtggct tagcattctg atcggtcatg agacctttct ccaggcccac aatgcgagga
     4561 acgcacgcag acaaactgat tctacatgaa tgagcggtgg cttgaaagcc gcctggcgct
     4621 tattgccagc cgcctcccag ggcacgatag agctgcacga cagcgctgcg ctcgcgtagc
     4681 tgcaactggg ccagcgtcaa ttcaccggca aacaggttcc tttgcgcatc caacacttga
     4741 aggtagctgt cgagcccgcc tttatagcgt gcctcggaaa gctctacggt ctccgtcaaa
     4801 gctcgcacca actgagtttg ttcggcgcgc tgcttacggg tgccgtcgaa ttccgccaat
     4861 gcgtcggaaa cttctcgaaa accggtgaag atggtctgtt gataacgaac cagcaactcg
     4921 cgctgttgcg cttccgtcag gcgcacctgg gagcggatct gtcctgccct gaagatgggc
     4981 agcagcgccg agggagcaaa gctgtacacg cgggccggcg cggtcacaat atccagaagg
     5041 ctgcggctct gccctcccgc gaatgcggtt aacgagatct gtggaaaata gagcgccttg
     5101 gccgccccga tctgtgcatt cgcagcgatc agattttgtt cggcagcccg gatatcgggg
     5161 cggcgttcga gcaggcttga cggaatgccg gcgggcaact ggtcgggcac ttgaatctgt
     5221 tcgagcctgc ggccgcgaac ctgggccgac ggcgcattcc cctgcaacag gctgagcgca
     5281 ttttcggttt gtgcgatgtt ccgctccacg gcggcgatct gcgcggtggc ggtgtagaga
     5341 agctgctccg cctggcgcac atccagtcca ctggcggcgc cgcggtcgcg ccgcagacgc
     5401 acgagcttca aactgttctc cgcgacgtcg cgtgtcttgc ggctgatggc gagttccaga
     5461 tcctgctcca gaaggaggaa gtacgtgctc atcacctcgg aaatcagcga cacggtgatt
     5521 gcgcgacggc cctcctcggt ggccaggtac tgcgcgcggg ccgattcggt cagccttcgc
     5581 agacggcccc acacatcgag ttcccaggac agctgcgcgc cggcctccgt gaacgacgcg
     5641 ctaagatccg taccccgggg cacgaaaacg ttggcgccca ccgaagagtt ccgcacgctg
     5701 ctaaatccgg cgccaatgtc gagaaacggg aactggttcg cgcggttgat gccgagttgc
     5761 gcccgagcct gttccacacg ttccgccgcg acgcgcagat cgaagttctg ccgcaacgcg
     5821 gcgctcacta tgtcgttcag ggccgggtcg ggaaacaggt tctcccactt cgtatcggcc
     5881 agcgaggcgg aggaaccgct tgccgtctgt tccgccacgg cgccgcggta tgcggtggga
     5941 gcagcgacct gcggtctctt atagttcgga cccaccgcgc accctgttag tgccgtgaga
     6001 gccgccagcg tgaaaaatac cggtttcact gagcggcctc cggaaccgga gcggggccga
     6061 tagcctcccg tttcttcttc tggtcgacaa acttcaccac cagcacgaac aaaaccggta
     6121 cgacgaagat cggcaataat gttgcggcaa gcatacctcc gaagacaacg gtacccagcg
     6181 cgcgccgcgc tcccgaactg gcgccggtcg cgatgaccag aggcagcacg ccaagaatga
     6241 aggcaaagga cgtcatcaga atcggccgga gccgcaactg ggcggcttca acggccgctt
     6301 tacctatcga gtggccgtgt tcatatcgct cgcgggcaaa ctccacgatc agaatcgcgt
     6361 tcttcgccga taacccgata agcgtgacga taccgatctg cgtatagata tcgtagggat
     6421 acgaacgcag aaagatggaa gttaaagcgc caagtatccc gagtggaatg gcaaacaaca
     6481 cagcgaacgg aatgctccag ctttcataca gagcggcgag cgacaggaag acgagaatgg
     6541 aggagaaagc aaagatggcg gcctcgtttc cctgcgcctg cttttcctga aatgtagtgc
     6601 ccgtccattc atagccgaag cccggcggaa gcgtggcatc cgccacttcc tgcatagcgt
     6661 cggcagcctg accgctcgaa tagcccgaag cggcgttgcc cataatgtag atggaccggt
     6721 agcggttata ccggtagacg gcttccggac cgcccgtggt ttgcaggttc gtaagcgttc
     6781 ccatgggaac catttcccca tcgcgggtgc gcacgaagaa gcgatcgatg tcggtgggtt
     6841 cgtcgcggaa ccgcggctcg gcctgcatac ggacctgcca ggtccggccg aatacgttga
     6901 agtcattcac gtacaatccg cccaggaacg tttgcagagc gttgtacgcg tcggttaccg
     6961 gaatgccgag cgtctgcagt ttatcggtgt tcatttccac gttatacgtg ggtaccgacc
     7021 cgcgaaacgt gctgatgaca ttggccagct cgggccgttg ccgcgccgcc gcgaccagtg
     7081 catccgcggt cttcgacaac gtttggatat cggtgccggc gcgatcctcc aacatgaact
     7141 gaaagccgtt tgtggtactg aggccaagaa tcggcggcag cccgaaagcc agattcaaag
     7201 actccggcac cgtggcaaac cgcgcatagg cctccgcgag cactgcctcg agttgcgttt
     7261 cgcgcgtctt gcggtcttgc cacggcttca aacgcccgat gacggtcgcg acatttgagc
     7321 tggaggtctg cgtgacgatg tccagaccgc cgattgtcat cacgccctcg acgccgggaa
     7381 tgcccgcgag gagcttctcg accttgcggg cggcagcgtc ggtccgctcg gtggaagcgc
     7441 cgtcgggtaa gcgaatcgaa ctgaagaacg caccctgatc ctcatcggga aggaagccgg
     7501 tgggcagtgt atggaaaagc cagccggaag aaaaggcgac gaacagcaga aacacaataa
     7561 cgagcgcagg acgccggatc aggtggatca cgccacgccg gtaccgcttg ttggtgcggt
     7621 tgaaggcccg gttgaacgct ccgaaaaact tgccggccag cccgccggat ttccggtgcg
     7681 ggcggagaat catggccgac aaagcggggc tcaatgaaag tgcgctaaat gcggagatca
     7741 aaacggaaac ggcgatggtg agcgcaaact gtttgtatat ctgcccgctg atgccgccga
     7801 tgaacgcgac gggaatgaac acggcggcga ggataaacgc gatggcaacc accggcgcgg
     7861 aaacctcatt catagcgcgg atagtagctt cgcgcggagc catgccgtca tcgatgtggc
     7921 gctgtacggc ctcgaccacg acaatggcgt catccacaac aatcccgatg gcgagcacca
     7981 gtccgaacat gcttgtgatg ttgatcgaga atccgagcag cgggaagact gcaaacgttc
     8041 caataatcga taccggaata gtcagcagcg gaatgagcgt agcgcgccag ctctgaagga
     8101 agatgaagac caccagcaca acgagcgcta ccgcctccat cagcgtcttt gcgacatccg
     8161 tgatggcggc gcggacaaac atggtggagt cgtagggtac cctgtactcg atgccgggcg
     8221 gaaacgcctt ttgcgcttcg tccatgaacg ccaccacctt gtccgcggtc tccaccgcat
     8281 tggcacccgg agacaggtaa agaatgacgg cgccggaagg ctgcccgtcg aggcggctga
     8341 agccggcata gctttgcgct ccgagttcca cgcgtccgat gtcccgcaca cgcaggagcg
     8401 aggcgtctgg ctgagcgcga accacgatat cttcgaactg actgatgtcg ctcaagcggc
     8461 ccggcgcccg caccgcaaac tgcaagtcgg tcccctgggg tgtgggagcc tgcccgaccg
     8521 cgccggccgg gttctgccgg ttttgtgcct ggattgcagc ggcgacatcg gtagcggtaa
     8581 gcccgagctt tgacatccgg tccggatcga gccatacccg catcgagtag gtttcctgcg
     8641 aggcaagcct ggattcgccg acccccggaa tgcttccaag ctggttcagc agattgattt
     8701 gcgcgtagtt ggccagaaac tgggaatcgt aggccttgtc gttggaaata agattgatga
     8761 ccaggagcag ttgactcgat accttctgca ccttgacacc ctgacgctgc acttcctcag
     8821 gcaagcgcgg caaggcggcg ttcaccttgt tctgcgtttg tacggtggcg atgtccgggt
     8881 ccgtgccgag ctcgaaagtc accctgatgt tcatctggcc atcattcgag ctgatggact
     8941 ggtaatagag cattccgtcg agaccgacca actgctcttc gatgggctgt gcaacggttt
     9001 tttcgagatc ctccccattg ccgccgagat agacggccgt gatctgtacc acgggcggca
     9061 ccacttccgg ataggtcgcg atgggcagaa atggaatgga gacgccgccc agaatgagga
     9121 tgacgatcgc tagaacgatg gcgaagacgg gacggttgat gaacaggcgg gccatctgtt
     9181 accccacctt tccgggtgat gccgcgggcg atttggcggt ccagggcttc ggagtcacgg
     9241 tagccccagg ccggatcgtc atgaggccct cgacgatgac gcggtcgcct gccttgagtc
     9301 cctcttcgac caggagggag tcgcccacgc gctggcctgt cttgatggag cgcgcctcaa
     9361 ccttgttgcc agggccaacg atataagcaa gttgcagatt ctgattctgc tgaacggccc
     9421 tttgcgggat gactaatacg ccttcgcgcc gctccgcgat gtactgaatc ctgccgaact
     9481 ggcctggaag cagggtatgc ccgggattgg gaaattccgc ttgtagctcc agggtgccgg
     9541 ttttcggatc gacctggttg agagcgttgc gaatacggcc gatgtgggga aactccgtgt
     9601 tgtcagccaa tatcatacgc aacgcgggtc cttcgtcctt ggagccggtg tatcgccggg
     9661 tcagcgcgag atgctcggat tcgctgactt tgaaacgcac ccagatggtg tcgagcggaa
     9721 caatcgtagt caacggctgc ggagagttcg cggtcaccag gccacccacg ggaacaaccg
     9781 aatcgcccac aatgccactg atgggcgcac ggatcgtggc gtattgaagg ttcaaggaag
     9841 cggtctcgag tgccccgcgt tgagcctcca cctttccttc cgtcgattga atctgcgttc
     9901 cggtagtaag gtgggtctgc tccacgtttg ctttgtgggc gcgcacactg gcctcggccg
     9961 cacgcaagtt ggctaccgcc gcgtccagat cctgctgggc ggctgcgtcc tgttgcacga
    10021 gaggctgcaa ccgctcgtaa tcctgacgcg ccttcaccag gttcgcttcg gcggacgcca
    10081 ggttggcttc cgcttccagc agagagactt gttggcgggc gaaggtgagg tcggcctcac
    10141 tctggcgcag ggcgccctgc gcctgctgta cctgggcctg gtaaggacgg aggtccagga
    10201 cgtaaagcgc ttgaccctgc ttcacgaact ggcctggacg gaacagccat ttatccacgt
    10261 atccgtcaac ccggccgcgt acctccacca tgttccgggc gtaggtttga gccggatact
    10321 ccgcaagcag ttccgcggtg gtgggagcaa gttccaccac ttccaccagc ggagggtggg
    10381 cggcttcgcc agaattcttt tgggccgaga cagacctgca tccggccaaa ccgaaagaag
    10441 ttaccgtaat cgtgagcgca aacagaacgc tacgggaacg gcgatggctc aggagagtgc
    10501 ggggtaaaga tgggaacata aagttctttt aacgttactg actacccagt catttggttg
    10561 agatgatgaa tatactccaa agttgcaggt tttttcctga ttgcgctggc agtacagttg
    10621 caatacgatg accttgtggt cacaagtccg gggatcaaag ggcgtgccag ccgcgcggat
    10681 gcggccaaaa gccgcaggca ggctatttta aaggcggcgc gcacggtatt tgcccgccag
    10741 ggctatgcga atacactgat cgacgatgtc gcggcagccg ccggaatcgc gaaagggacc
    10801 gtctacctct atttcgcctc gaaagaacag atctacctgg aggcgctgct ggagcgggcg
    10861 cgggaggttg aggcgcggtc ccgcgcaagg atactggaag cggcgacctg gcaggaaaag
    10921 atccgcgcat acatggaaac ccgtgtggag tatttgaatg cccatcagga ttttttccgc
    10981 atttacgcca cagagttccg gaatatgtgc atgcagggca aagcgatcgg agcggagttg
    11041 caggacctgg tccggcaggg tgaacaacag gtagcgcaag tgcttgccgt cgccgcggcg
    11101 aaaggcgaga tacggcctct agaccccgac ctgggggccg cgatgatttc cgatctgatg
    11161 cgcggcctgg tagaccgcac gctattggcg ttccgccgca ctccggtagc tgaagacctg
    11221 gaatttacgc tcgactgcgt gtaccgggcg ctggcaaaag aagcgaatcc ggcctgagca
    11281 gttttcattc gccggtggct ctcgaggaag cagacctgat gcaggggccg cgtcgctatc
    11341 atcagaaaat cgggctgaat ttcgtggatc cgggtgtata ctggccggga acttgaaagg
    11401 tcgaactctt tgatccgcct ttcgttggcg tcctgtcctg ggaatttagg tctgtttacg
    11461 gaggtcctcc attggccaca aagaagcctc gcaacggaaa tgggaagtcc ggtgagaagt
    11521 taacagttgc cggtgcgcaa aagctttcga cggaagagct tgctctctac atcaagtccg
    11581 ccttgataaa agaggctctt gaagatcccg cgtttaggaa actggcgttg aaagacccgg
    11641 catcagccat tcaaaaaagc cggctgaccg gcggcgttga aaaagccctg cccgcgggat
    11701 ttactttccg cgtcgtggag gaaacgcaac aacttctcta cctggtcatt ccccacaaaa
    11761 tgccggaacg gctggatccc aaaaacccca agcacattct gctcaaaaag gccgtgagcg
    11821 atccccaata tctgcagaaa cttacagccg atcccaggtc cgttctccag tcggaatttc
    11881 tcgttgacgt tccgcctggc tttcaaatca gggtcataaa ggaaacaccg ctcgaaagtg
    11941 ttgcagtgct tccagcggac ctccggccgg agcaaataac ggcaattccc aaggaactga
    12001 tcgagtatca agcgagcggc tggtgcggca agacgctcaa gacgatcatt gaaaatctgt
    12061 gtccgaaacc gccgggagaa acggaattcg aaagtccttt ctgtacgccc gattgcggag
    12121 tacagccatt gtgacgctcc gcgcttgatg tagctggttg gtgtcgaatg tcccgtaaca
    12181 ggcgctccct ggccgaatcg cttgaggtcg tcgcccggta cgttccgcgg actctgatcc
    12241 ggccgcgcca gatgcaagcc atccgagagc ttgccggcct cctgcctcct atcgcggttg
    12301 ccggctttga gtgccgcttg gcggaggtcg gcggccgtgt cgatttcgca gtgtttctgc
    12361 cgtcatccga cagttgcttt tctgtcgtcg cagggatgaa cccgagccac gagctggctc
    12421 cggagcttct ggaagacccc acctggcagc acatccgaac cttctgccga agcgtgcacg
    12481 aaccaggagg cccactacat ggcgttgtcg acgacgtttg gctcgaattc gatttgccct
    12541 cgaatgggaa tccaactctc acgcccgctc tgttcttcgg cataagccgt gctgtgacga
    12601 gttcgcccgg aaacagcctc gaaatcgtgg agtctactct gaagtcactc ggggcgccgc
    12661 cgcttgccgg aatcactcag tctgaatttt tgagggcagc ttcctgcctt ccggtggact
    12721 ccgtcatctt ccaaataggg attccccttg cgtatcgcgc cagcgcgctg cggctctgca
    12781 tttccggcct gccgccgggc gggatcgcgc cttatctgcg gaaaattgaa tggcgtgaca
    12841 cgccattagg tctggagaat acgatcacgg atctggccgg cattgctgac gagttgttcc
    12901 tggacatcga catcggcgag atatccacgc gcaagatcgg catcgaggcc cacttccggg
    12961 ggccgaaaag acccataatc gatcgccgtt gggaggacct gctgagctgg ctggtccgtg
    13021 aggggctttg cgcccctgaa gtcgagcgcg atatccgcag atggaccggg acaacatact
    13081 tcggcgaagg tggagcccct aagccgaagg aactgctggc tctgacgaag ctgcttgggg
    13141 gtcgcgcgac gctctgcttt ctcaggggac ttcatcacgt aaagatctcc tacgaacctc
    13201 gtcgaggctt cgaagcaaaa gggtacattg gcttcaagcg gcactggatc gagacaatgc
    13261 ccgccaattt gccggcactc aagcgcagcg gcaggcctcc cttcgagagt tcggttaggg
    13321 atacgctcgc tgctggggta gaattcctcg ccggtagccg cgacccgaac ggatggtgga
    13381 aagatttcga actggctgcc ggcgccagtg acacctgggt caccgcttac gttggctggg
    13441 cgctcgccgg cgtggatgaa cctcagagct gggaaatggc tcatgccgcg tggtgtctgc
    13501 tcaaacagag cctccacccg cacggcggct ggggttacaa tctgaaagtt tcacccgacg
    13561 cggatagcac agcctgggcg attttgctgg gagagagcgt cggagattcc cgttctctgg
    13621 cactccgaac ggcgaagaat ttccttcgaa gtcatctgcg gacttccggc ggagttgcga
    13681 cctatgccga agaatcgcca gtccgcgccg aaatgcgctt tccagccgag gtgtcatatg
    13741 ccgggtggac gacggcacat ccgtgcgtga cggcgttggc cgccaacatc caatccttcc
    13801 gcgcaaggct catccctttt ctcttgagaa gccagagaaa agatggaaac tggcccgcat
    13861 actggtggat ggatccggaa tacgcgaccg cgctcgctgt cctcgcgctg aacgggatcg
    13921 aaggcttgac ccccgagccg gcttccaggc tgcgcaaagc cgtccgatgg attgaattgc
    13981 ggctcgcgga gcatctctcc ctgatgccgg atgcaagcgc gccgcaggtc tttgccatcg
    14041 cctggtgtct taaggccctt gcagcttgcg cggtttccag cggtcaggcc gccctgattc
    14101 aacaggccat gaatcggctc atcgatgcgc aactacccga tggctcatgg actgcttcgg
    14161 cacggctccg ggtcccgccc accgattgca tgaatccaaa tcgaatccac agttggtatc
    14221 cagggggcaa ggcgattcgc ggcatttctc ttgatcagca aaggacgttc acgacagcaa
    14281 ccgcggtaag cgcgatcagc gccggagctg agcgactggc ggagttgact cattgacatt
    14341 ccatgatctg gctgtcctcg ttgcagcctc cagcagcctg tacgagcgaa tacagaaagg
    14401 ctttcaaccc gtttcaaatc tcaatccgga ctccagcacc tctcaggaac tcgctcgatg
    14461 gaagcagatc tacagggaca aggggccagg agcattcaag cgccgtcttg cactcgacgg
    14521 ctggaccgag agtttcgttc aaccctatct cggcgcggta cgtcttcccc acgaatcgga
    14581 tctgcccgca tgggctttga ccttgcgggg gtgtgtcaat cgaatgctgg aatccaaacc
    14641 aggcgatttc ctcgcgagtg gcgacgaatc gccacaaccg cgtctctttg agcatctctt
    14701 taccccgatg gtgcagtttg cctctctgga attgaggcgc agggcggggg acaagagccg
    14761 gcttctctcg accaccgcgc tcgccggcgc agaattgtcg ctattggatt cacttgtgca
    14821 ggtgtccata ggtaccctcg cgcttgagtt tcaggcgttc cgttcagcca gggaggacac
    14881 aattttccgg atactacgcc gaatgaagga tcagcgctcg aacgagcttt accgcgcatt
    14941 cctggtccag atgcacgcag gagggctcct gaatctgctc tcggcgtatc cggtactcgg
    15001 ccggttttgg ggactgttgg cggaattgtg gattgagaat ttgaccgagt ttctcacccg
    15061 gctcgatacc gatttgccca ccctgagcag acgtttgaaa gtgagctcgc gccggccggc
    15121 ggttgccaat cttcgaatga atatttccga ccgtcatcac gggggcagga cggtcatgct
    15181 ggttgtcttt tccggagaac ggcgagtcat ctacaagccg aagggactgg gattggaaaa
    15241 ggccttcttc caattgctct cttggctcaa cagggaggga tcgccggttc aactccgcgg
    15301 tatcgaagtc atcgacagag agacatacgg gtgggtggag ttcgcggatc accgcagttg
    15361 tgtcaatcgg gatgaggtag cctcttatta tcgtaaggct ggagggctcc tctgcatcgt
    15421 acatctcctg gagggcacgg attgccacct cgagaacatc gttgccgcca gggccgatcc
    15481 tgttctggtc gatgctgaga cgctgctgca tcaccgcgtt gtccgcgacc gtagcggcct
    15541 tgaatcgggt ttcgaagccc aggatgaatg ttgggactcg gtgtttcgga ccgggatgct
    15601 tccgagctgg attctcgaag ccgacggtac tccgttcgac atcagcggtc tgggcggatt
    15661 cggggagcag gcttcgtctc agcgggtacc gcattgggac aacatcaaca ccgatgagct
    15721 tcaattgcgt tcgctcccgg gccgccggag gcctgcccgc aacgttccca tgctggggga
    15781 aacggctcag tcgccccttg ctttcgaaag cgagctgatc gccggcttct ctgaaatgta
    15841 ccgtttttgc gaccggcaca aggctcttct gactgcgcga aagagccccg tccttccgct
    15901 cgggagccat tggagccgct acgtcgcgcg cgacaccacg gtgtattgcg aactgcgccg
    15961 cggcctgctc cagccctcgg cgcttacaag cggaatcgcg caaaccctcc atctggaact
    16021 cctcgccgcc gcgcatatgg ccaatacagc gcgacccgag ccgtggaaga tcgtccgttc
    16081 tgaacgcgaa gcgctacttc aattcgatat tccctatttc cgcgccaggg cctgctcacg
    16141 ggatcttgag ttgccggacg gaaagctgat cgccggcttt ttcgagagag ccagtatcga
    16201 gcgggtattg cgcaagattg ccggtaccag cccggaggat cttcgcaaac aggtccggtt
    16261 cattcgagcg tccttctttt cccggtcagt acggacacct catcagcgga actccgtcag
    16321 cacgcttcgc agaaaggatg tcccgggtac gagcgccttt cgtcaagcgc agttcatcgc
    16381 cgaggccgag agaatcgccg aagaacttcg gaaagattcg gcccgcgtga acggcacggg
    16441 cgcggcgtgg atcggcctcg actatcggcc ggaatcgaag cgatatcact accagccggt
    16501 gggcaacggg ctttacagcg gccgctgcgg catcggtctg tttctcgccg cactttgggc
    16561 aactaccggc gagacgcgat ggcgggagct tgcgctgggc gcggttgatg gggcgcggcg
    16621 ccgcatcact gagaaagggt ggcaggttgc cgatgcgagc atcgcgtatg caatatgccg
    16681 tatcggaact tttctggctg atgaagatct gcttcgtgac gcgacgaagg ccgccggccg
    16741 gattgggcgg ggggcgatag catccgatga aaaccttgac gttctattcg ggaccgccgg
    16801 ggcgcttctc agcttcctcg ccctacacaa atggtgcggc gatgaagcgg ctctcgcgaa
    16861 agccgttgcc tgtggacggc gcctgctgct cgctcgtgtg cggacgggga ccgggcatcg
    16921 tgcctggcag tctcgcgagc ttcctgcgct caccgggttc tcacacggcg cggccggcat
    16981 cgcctatgcg ctcctgaagc tattcgagac caccggccag gagtcatttc gcgaagctgc
    17041 gctggaagcc attgccttcg aacgtgccgt gttcgacgcc gaagccggca actggcccga
    17101 tttccgttcc ggagaaggac ggcagggaac ccgcggcttc atgacggcct ggtgcaatgg
    17161 ggcgactggc atcgggctga gccgtctggg tgccaggctt gccatagcgg acgggccggt
    17221 cgcacaggaa atcgaggccg ctctgagggc cacaaggggg taccgttacg acgatgtcga
    17281 tcatctctgt tgcggcagct ttggaaggat cgaattactt cttacggcgg gttgccagct
    17341 tcaccgtccg cgcctggtgg cagaagcccg gagatgggct tctgtgcgta ttcaacaatc
    17401 cgaacgcaat ggcggctatc gcgtgatgca ccggctgccg cgctgggtgc gaaatccagg
    17461 cttctatcaa gggatttccg ggatcggcta ccagttgttg cgactcgcac gacccaaaga
    17521 tctgcccata ctcctactgt gggagtgaga tttctggctc tattctttct gaagcgatac
    17581 gcgccgccgg ctgagcgtgg gtccggagcc gtcgccggca tgcgcggcgg ccttgagcag
    17641 gaaacttccc gcaacatccg gaatcttcat gtcgagcaga agcgtctcct gacctagagg
    17701 ctggattgaa tacttgcgct ccacacgcgc cacggcctcg ccgccctcgt tatcaagcgt
    17761 cagaacgaga ttaccgctgg ccggcgcgct ggaatcgttc accatcatca cagtgaacct
    17821 tctctccgca cccgccttga gattcggctg ccagaaattc aggtacacgc ccaggggttt
    17881 aaacgcctct ccggcgtagt ccgcgaaggt gggatccaac gtcagagact tcacgtcgct
    17941 gaagtggtcg catgtatagg cgccttcata gcaggatgtg agatagacga aatgaagtac
    18001 accggcgtaa ttgcggtgtg cgcgccagaa ctcggtcagg ccgccgagca ggtatccgtt
    18061 taacgccaac cgctcctttc cggtggccgt cttacccacc aggtgctcat agacccgttc
    18121 ggtgagcacg gtaggcgtac cgtcgcgaag cagccagatc cagccatatt cgttgagcac
    18181 catggcatgc ccgcttggtg tgaccgtgtt gcggccgctc cccgacatgc tttcgagcga
    18241 ggtcacctgg aatgggttgc cggtgcgccc gctgacgaag aggtacgggt gatcctccac
    18301 ggggtcgtca ggaccagcgg gcggattgta gctgttttcc cagggacggc gcgacaggtc
    18361 cagatcgcga acggccggga tcaccttctc gccaaagacc ggatcgtagg attcattgtt
    18421 cgcatcccag atcactacac tcgcatggtt ccagttgtcg cgcatccaat ccttgtactg
    18481 gcggatcagc tcgtccgcat cccactggcg cttatagttt ccggacacgt ggccggtcca
    18541 tatgaagaat tcgttctgaa tgagcagccc tgtttcgtcg gcgagttcga gccacttgtc
    18601 cggaaccggt ccgatacaaa accggaacat gttccagttc atcttcttcg gaatttcgga
    18661 gagcagtttt cgcacccacg tttcgtccca cgtcaggctg cccgcgtccg ggtcttcgaa
    18721 aaagcggtgc agcgtgatgt tggaaccccg caggaagtac accttcccgt tgagataggc
    18781 gcgcttcgta ggagtatcga atcgaaactc gcgaacgccg aatcttgtgg aagccgtatc
    18841 gcctccagtg gacgagtcga gaacgtaaag aaatggatcc tcgggcgacc acaggcgtgc
    18901 accgggaatg ggaatcgttt cggtgaccgt cttttcttca cccggtttaa gtgagacctt
    18961 ttccgccgtg gactgactcg ccctctgccc gcccttccac gccttcacct gatgacgtag
    19021 cgtggtagtg gccggtgcag cgccgtagtt cttcaacctg gtctgaacgg taatccgtgg
    19081 cggggtaatg gcgggcgcaa cctggatggt ttcgatcacg gggttgtcca tcagcaggac
    19141 agagacgctg tcatagatgc cgggcgtcca caggttcttc tcgaagtcgg agccggccgg
    19201 ataactctct ggcaggacgg cggggtgcgc gcctatccgt acgatcagac ggttctcgcc
    19261 agcccaatcc atcgcctgcg tgagatcgaa gtacgaagcg ctgaagcacc ccgcgtattc
    19321 gcccagtttc ttaccgttca gccaaacggc ggtcccgaac tgtgccttgc cgatgcggag
    19381 cagggctacc tgcttgcggg ccggaacgcg aaacgatttt gcataccaga agtagtttct
    19441 gttctgccgg gatatgccct gaccttcgag cgatgtcgat gccggaatta ccttccggcg
    19501 gatgcggttc cacaacactt ccttaccgtc gaaacggtcc acgtcggcaa aggccggctg
    19561 ggccaggttg gcaaggcccg gaaccggcac gcgatgagtc cagttgcgcg gaacggcgtc
    19621 cgcatccaca ctatcctcga tctgccattc gccatccagc gagacagtga tccgctcggc
    19681 caatgtcgaa atagaagtca ggaaaaagaa cactgcgaag aaaacaagtg agcgcatacc
    19741 ccctgttata cagccgcagc ccgggaagca aaagtagcga tgttattttg cggcggacgc
    19801 cggtacgttg agccaccgct tcaggaaggg aattcctgtt tcgagtacga tctcgtgccc
    19861 accctcgtga acattcaacc cgatgcggtc ttccaggcca agtttccggt agtgttcttt
    19921 agcaacttcg aactcttcca ccacctgcgg ccagtgcgcg atgcggtcct tcttcccgtg
    19981 ttgcaccagt agcgggcgcg ggcaaatcag gctggctacg tcgctgtcgg tgaactcggt
    20041 cagccatccg ttgaagaagg catgctcttc cttcgtatcg aggaagcaac tgtagcgctc
    20101 gtcgggaagt gccatcttgt tccggcgatg attgaaccac gcggtgatga tgctgcactt
    20161 gatgcgcggc tccagaggag tccagaacat cccggccatt ccgcccaggc tgatgcccca
    20221 gaaacctacg cgctcagaat cgacgcgcgg atctttgagc gcgtaatcga gcagtgcctg
    20281 catgcggcgg aactcgatgc ccggtagtgt cgtgtccgcc agccgcgcca gccgctcgat
    20341 gcgattccgc ttctccacgc tatagagatt cgccggcgcc aggaccgcaa aaccctgctc
    20401 caccagcgcg tggccgtaat cgtgatacgc gttccctgga tcgtctacac cgaacacgcg
    20461 ctcgggaaag ctgccgatac cgtgctgggc aatcacgagc ggcacgcgtc cggaggtgtt
    20521 cttcgggaat accagcagtc cttccgcttt caacgatccg agcggcacgg aaatccattc
    20581 ggcctgtggc aactcgcgga gaggccgcgg gcggcgctgc accggtccgg tgacgcgcaa
    20641 ttccggcggc gagagaaact ttttatatcg atcgcgattc ggacctacac tcttcagaaa
    20701 cgcctgttgg ctcgagtagt cgcgcttcca cgcgcgcgac gcgcgttccg ggtactgttc
    20761 gatgaggtag ttttgcaggt gctgctcgag ttcacggcca taggcgtcgt ttcgttccac
    20821 gatatccgcc ggtgttcctt gcgcggcgga tgtcggggcg ggagcagcga ccgacgcggc
    20881 catgacacgc accaggactt cgccaaactg cagccggtct ttaactcccg gttcgcgcga
    20941 gagccgcggg ctcggctcca ccatcagctc catcagaaag gccggaattt tgcggtcgtg
    21001 aaacagtccc tggttcaccg tcatgcggcc tttcatgccg gcggtggtcg tctcggtggt
    21061 gttccgcggc cccttgggcg agtagaacac cgtcttccca tccagtagtt tccagaaacg
    21121 ctgccccagt tcttgcatcg gggccccgc
//
LOCUS       DASTMK010000544        11479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_187427, whole genome shotgun sequence.
ACCESSION   DASTMK010000544 DASTMK010000000
VERSION     DASTMK010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187427"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2155
                     /locus_tag="VFQ79_22515"
     CDS             <1..2155
                     /locus_tag="VFQ79_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142513.1"
                     /translation="PRPRNGDMYMIRGDWNLSAKHILSGQVFVDRNRMIRPELVSGNV
                     PGYLGTFTNQQTTMAVLNATSTFSPNLLNQLTVTFLRPVHESNADNTVPHEQIGVNMP
                     LYTPTGRLGVSISGGPNFGSRSVYTHVSNNWQFRNQVSWTRGRHNLKFGGEWLHLTNL
                     QIFLGATSMSFNGSRSGDPVADFLLGAFRTVSGGFGVRTNDNYQDAPSFFFQDEFKVH
                     PRFTLTYGVRYEPFFPWVDKYDRLTSLAAIGTNVRSTRFPDAPPGVLFAGDPGVPRGI
                     SGPDKNNFAPRFGFAWDLFGDGRTSLRGSYGIFYDYIKADSVSQEGAPWAGNFQIFDG
                     RAENPFGSLGLTPPPVVPGEGGFGCVEIPTFPGQRCSLFPLPMAGLFVSSDLTTPYIQ
                     SWNLTLQRQLTSDIMLQGAYIGKIGTHLDGWWSPNPARYINDPITGAPPSLQNVNNRV
                     SLAPGILAPNVYELTTNFRSWYHSFQTQVIKRFSRGVSVSASYSLAKSIDIVRQNIGN
                     WPLANPFDMNSNRGRSDFDRRHAFVASWLWSPTRKWNGAFQRTLLEDWTFTGIHTLQS
                     GAPFTVYMGDDVAQDGVSGGQQHAMLQPGATVTRDHANRADMIARFFNTDAFIPTNQV
                     PRGVYGDSGRNIISGPGMSVTDFSAIKDFRVNERFRLQFRSEFFNLFNQVRLGCTDTT
                     WGCSDPVAVVNASGFGQIRSAGPAREIQFALKLLW"
     gene            complement(2300..3313)
                     /locus_tag="VFQ79_22520"
     CDS             complement(2300..3313)
                     /locus_tag="VFQ79_22520"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU0142514.1"
                     /translation="MNRRVFMAMPGAFAPLQKTGEAIRPKALKPGDTVGLITPATYVS
                     DPDALATAERTLRHFGLEPKFGANVRRRNGYLGGTVQERVEDLHAMFRDPDVKGIFAI
                     RGGYGSGQLLDRIDYDLIRKNPKVFLGYSDITTLLIAIRQRAGLVTFHGPVVLSRFSD
                     YTQNHFRKTLFNAEPVGVVTNPPESNTLRPVHTLRTIRPGKASGELIGGNLSLVTATM
                     GTPYEIQTAGRILFLEDVGEEPYSIDRMLTQLRLAGKLSAAAGVVFGECRNCHPRECG
                     PSFESTLSVGEVLDSILGEIKVPVLTGLTIGHTEDQLTLPEGVQATLDADRGELTIGQ
                     AVA"
     gene            complement(3323..5356)
                     /gene="pheT"
                     /locus_tag="VFQ79_22525"
     CDS             complement(3323..5356)
                     /gene="pheT"
                     /locus_tag="VFQ79_22525"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU0142515.1"
                     /translation="MKFSYNWIRELVDGLDIDPKHLEQLITIKTAECEGIEPSQGSQP
                     GCTPDSIIEIDNKSITHRPDLWGHAGMAREVAAITRKPFLDPVRVDLVPAGSSPARIS
                     IEDFELCPRYSALVFENVSVQPSPAWLQCRLEAIGLNPINNIVDVTNYVMAEIAQPMH
                     AFDADKLHGDTIFVRRAHVGECLAALNEERYELSAGNLVIADAKGAIALGGVIGGLES
                     AISGATTRIVLESANFQAVSIRKTSAGLKLRTDASMRFEKSQDPANTVRGLARAIELL
                     NEVCPGIRLIGGLADQARVIIPPPAILLPLDWLVRKLGLAISEEEVRDILERLQFGVE
                     ASSPGVLSVTVPGWRATRDISIKDDLVEEIGRMVGYDTITPTAPLVTATVPPANEERH
                     FHHRVREMAAAQGYTEVYNYSFISGEMARAFHLDPNAHLEIANPINSDQRLMRCSLLP
                     GIWKNIEENSRNYDSFRLFEIGLEIHKREDASNLPDEIPHLAAVVYGRGDGVDGLFEL
                     KRLAECLMPGVGVAHCEPRPYEHPHRSVALRWHGNTVGRLFELHPSMVAGRAAILDVN
                     LQLMYKLQPGVAMYKPIRRFPSSAFDLSVIIGLRDEVGRVGELLAQFAGPNLEALEFV
                     RQYSGPPLAAGQKSVSYRMTLAAADRTLSADEVSSVRNGIIEAMRARGYDLRI"
     gene            complement(5369..6391)
                     /gene="pheS"
                     /locus_tag="VFQ79_22530"
     CDS             complement(5369..6391)
                     /gene="pheS"
                     /locus_tag="VFQ79_22530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU0142516.1"
                     /translation="MLESTLRDLECSARERIAAAEAPQSLEAVRIEVLGRKGTLAQIS
                     KELGKLAPEERARIGKLLNSVKQDLETELDSKKGQFDQEALRERLESEWVDLTLPAPG
                     TRPGALHPIARIQTEIEDLFISLGFAVLDGPEVETEYNNFDALNIPPHHPARDMQDTF
                     WLTDGHLLRTHTSPVQVRGMERMGPPLRMIAPGRVFRNEEVDASHEHTFYQLEGMMID
                     RDVSVAHLIFFMKTLLSAIFHRDVEVRLRPGYFPFVEPGFELDIKCLICGGSGCPVCK
                     QSGWVELLPCGLVHPNVLRMSGIDPEEWNGFAFGLGLTRLAMMRYGIDDIRLLQSGDL
                     RFLTQF"
     gene            6521..7456
                     /gene="mdh"
                     /locus_tag="VFQ79_22535"
     CDS             6521..7456
                     /gene="mdh"
                     /locus_tag="VFQ79_22535"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU0142517.1"
                     /translation="MRKKVTVVGAGNVGANCALRIAGKELADVVLVDVVEGVPQGKGL
                     DILQSGPVEGYDVSITGANDYEPTANSDIVVITAGFPRKPGMSRDDLLLANYEVVKTA
                     TEQAIKYSPDSILILVTNPLDAMCWTAQQVSRFSKNRVIGMAGVLDTARFRTFISQEL
                     DISVENITALVLGGHGDTMVPLVRLVTAGGIPLTELLDQAAIDRLVQRTRDGGAEIVR
                     YLKTGSAYYAPSAATVEMVESILKDKKKVLPCAALLEGEYGISGLFVGVPVKLGARGI
                     EKIYELKLTAEEKAMLDKSAAAVQELVDVIKQKMG"
     gene            7479..7925
                     /locus_tag="VFQ79_22540"
     CDS             7479..7925
                     /locus_tag="VFQ79_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEU0142518.1"
                     /translation="MAEQIRPYIDPKYAVFRLRVAKSGIHRWGVFAEERIPARRKIIE
                     YTGERISRIETKRRSSSREYVYLFTLNSYWCVDGAVGGSGAELINHSCDPNLYSCIIK
                     DHILYMSKRVIEPGEELTVDYHFAKDIETYICRCGASGCRGTINVK"
     gene            complement(7912..9192)
                     /gene="eno"
                     /locus_tag="VFQ79_22545"
     CDS             complement(7912..9192)
                     /gene="eno"
                     /locus_tag="VFQ79_22545"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU0142519.1"
                     /translation="MTLTIRQLRALEILDSRGNPTLAVEAQLSDGTTAPAQVPSGAST
                     GRHEAVELRDGDARYAGKGVRKAAANVNDILSAALEGADAEDRAALDRKMIELDGTSN
                     KSRLGANAILGVSCAVARAVAQARKVPLWQSLAGARTAVLPVPMINILSGGWHAGHNI
                     EFQDFLIIPHGFDNLAEALEASVAIHRQVFLLCRARGLTLTGVADEGGWGPLLSTNEE
                     ALQLLTEAIGSAGFEPREQVSIAIDVASSHFFSEGEYHLKTEGRRLTAAGMIALLRDW
                     CVRYPVVSLEDPLHEDDWGNWPELTAALGGGRQVIGDDFLTTNPARVREAIQQKAANS
                     VLVKMNQIGTLSETFEVLDEALAANWTAVVSARSGETEDSFLADLAVASASGQIKIGS
                     ITRSERLAKYNRLLEIEARWRLPYPGRELRFISR"
     gene            complement(9189..10178)
                     /locus_tag="VFQ79_22550"
     CDS             complement(9189..10178)
                     /locus_tag="VFQ79_22550"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0142520.1"
                     /translation="MFELTLSTAHEYVKAHCGTDAGAIGIRELGGGVSNTVLLVECGT
                     KRFVLKQSLEKLRVEQEWLSDRTRIHRECAAMEALGPHLPPDSVPRILFEDQANCIFA
                     MSAAPAHAATWKSLLLAGNIDTGIAAKIGQMLGCIIGESRKRPDWKARFSDQTVFDQL
                     RLDPYYRATAQRHPGLALFFERLIQKARTRRISLVHGDWSPKNFLVDGGSVMAIDFEV
                     VHYGDPAFDAAFLTNHLVLKTFHRPYWRSGYRSAALAFRDAVMDTVRELPEEFEQDTI
                     EHLAGLLLARIDGKSPAEYIQEEAAKARVRAFGLGLVADLPSSTEELFDRIPE"
     gene            complement(10213..11340)
                     /locus_tag="VFQ79_22555"
     CDS             complement(10213..11340)
                     /locus_tag="VFQ79_22555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142521.1"
                     /translation="MPQVPGLAPVTEMRTYPPAEALGEFFSGRQVNPCFLDASTNKDT
                     ALALIADLLRQDPTLQIVVLLASTDSDFILRCLRQGATGFLTAPFTAGELEPVMERLA
                     RMRPETYQVSQAGGKVYCVMPAKGGCGATTLAFHLAHQWKRNGSKRILLADLDPLTGT
                     VSFALKLTSAYSFMDALQRGDSLDVDIWKALVASWNGLDVLLSPEDPARGIGDLPDPG
                     HLIEFAKSCYDIVVLDTSGVYGNWNGQLAAHADEVLLVMTNELPAIQAGQRALSYLER
                     RKVDRSRIRVVVNRFSEELGVSGETIETALKLEVCHTMPSDYDAIQKATMEGKTVPPR
                     GKFGKSLAALSAAMTGGSELPDEEPKSSALGKVLSLFARRR"
BASE COUNT         2368 a         3452 c         3336 g         2323 t
ORIGIN      
        1 cccgaggccc cgcaatggcg atatgtacat gatccgcggc gactggaatc tcagcgcgaa
       61 acatatcctg tcgggccagg tcttcgtaga ccggaatcgc atgatcagac cggaactcgt
      121 gagtggaaat gttccgggat accttgggac cttcacgaat cagcagacca ctatggcggt
      181 actgaacgcg acctcaacgt tcagtcccaa tctgttgaac cagctcacag tcacattcct
      241 gcgcccggtc cacgaatcca acgccgacaa taccgtgcca cacgaacaga ttggcgtgaa
      301 tatgccgctc tacacgccta ccggacggct gggtgtcagt atttcgggtg gcccgaactt
      361 cggcagccgg agcgtgtata cgcacgtaag caacaactgg caattccgga atcaggttag
      421 ctggacccgg ggcaggcaca acctgaagtt cggcggagaa tggctccacc tgacgaatct
      481 gcaaattttc ctcggtgcca ccagcatgtc tttcaatggc agccgctccg gcgatcccgt
      541 ggcggatttc ctgctgggag cgttcaggac ggtcagcggc ggcttcggag ttcgaacgaa
      601 cgacaattac caggatgcgc cgagcttctt cttccaggac gaattcaagg tacatccgag
      661 gttcacgcta acttacggcg tccggtatga accgttcttc ccctgggtgg acaagtacga
      721 tcgtttaacg agccttgccg ccattggaac caacgtgcga tcgacgcgat ttcccgacgc
      781 gccgccgggc gtgctgttcg ccggggatcc gggcgtgccc agaggcatca gcggtccgga
      841 caaaaacaat ttcgcgccgc gctttggatt tgcctgggat ctttttggcg atggaagaac
      901 gagtctccgt ggctcctatg gtattttcta cgactacatc aaagcggact ccgtctcgca
      961 ggagggcgcg ccgtgggccg ggaatttcca gattttcgac ggccgcgccg agaacccttt
     1021 tgggtcattg ggcctgaccc cgccgccagt ggttcccggt gaaggcggct tcgggtgtgt
     1081 tgagatcccg accttccccg gccagcgctg cagcctgttc ccgctgccga tggcggggtt
     1141 gttcgtctcg agcgatctga caacgcccta tatacagagc tggaatctca cgctacaacg
     1201 gcagttgact tcggacatca tgctccaggg cgcctatatc ggcaagatcg gcacacacct
     1261 ggacggatgg tggtcgccga atccggcccg ctacattaac gatccgatta ccggcgctcc
     1321 gccgtcgctt cagaatgtca acaaccgcgt cagcctggcg ccgggcatcc tggctccgaa
     1381 cgtgtatgag ctgaccacca acttccgcag ttggtatcac tccttccaga cgcaggtgat
     1441 caagcgcttc tcccgcggag tttccgtttc cgcatcgtat tcactggcga aatcaatcga
     1501 tatcgtccgg caaaacatcg gcaactggcc gcttgccaat cctttcgaca tgaactcgaa
     1561 ccgtgggcgt tcggatttcg accggcgcca tgcgtttgtc gcctcctggc tgtggtcgcc
     1621 aacccggaaa tggaacggag cgttccagag aacgctgttg gaggattgga cgttcactgg
     1681 aattcacacg ctgcaaagcg gcgcgccctt cacggtgtat atgggtgacg atgtcgcgca
     1741 ggatggcgtc agcggcggac agcaacacgc gatgttgcag cccggcgcca ccgtcacacg
     1801 ggatcacgcg aaccgcgccg acatgatcgc ccggttcttc aacaccgatg ctttcatacc
     1861 gacgaatcag gtaccgcgtg gagtgtacgg agactcgggg cggaatatca tcagcggtcc
     1921 ggggatgagc gtcaccgatt tttccgcaat caaggacttt cgagtgaacg agcgtttccg
     1981 gcttcagttc cggtccgagt tcttcaacct cttcaaccag gtgcggctgg gatgcaccga
     2041 taccacctgg gggtgctccg atcccgtggc agtggtgaac gcatccggct tcggtcaaat
     2101 ccgaagcgcg ggaccggcgc gcgagatcca gttcgcattg aagctgcttt ggtaaggaac
     2161 cgtcaggaaa caaccgtgcc agttcgccaa gaccactcgc taacgctcgt ggctctgtaa
     2221 tcgtttctga gccgcgaacg tgagtgagcg gttgacagtc cctaagccga aggtcggact
     2281 tttaccggac aggtcgcgct caggctacgg cctgtccgat cgtgagttct ccgcggtccg
     2341 catccaaagt ggcctgaacg ccttcgggga gcgtcagttg atcctcggtg tggccgatgg
     2401 tcaaccccgt caggacaggt acttttattt ctccaagaat gctgtccagc acttctccaa
     2461 cggagagggt tgattcaaat gacgggccgc actcgcgcgg atggcagttg cggcactcgc
     2521 cgaaaacgac tccagcggcg gcggaaagct tgccggccaa ccggagttgt gtcagcatac
     2581 ggtcgatgct gtaaggttcc tcgcccacat cttcgagaaa cagaatccgg cccgccgtct
     2641 ggatctcata aggagtaccc atcgtggccg tgaccaggct aaggttgccg ccgattaact
     2701 cgcccgatgc cttcccggga cgaatagtgc gaagtgtgtg cacgggccgc agcgtgttgg
     2761 actccggcgg gttcgtaacc actcctaccg gttcggcatt aaacagggtc ttccggaagt
     2821 ggttctgcgt ataatcggaa aacctcgata agacgacagg cccgtggaac gtcaccagcc
     2881 ccgcccgctg acggatcgca atcagcagcg tcgtgatgtc gctgtacccc agaaagacct
     2941 tcggattttt tcgaatcaga tcatagtcga tgcggtccag aagttgaccg gagccatatc
     3001 ctccccggat ggcgaatatg cccttcacat ccggatcgcg aaacatggcg tgcaggtctt
     3061 cgacgcgctc ctgcaccgtc ccgccgagat agccgttcct tctgcgcacg ttcgctccaa
     3121 atttcggctc caggccgaaa tgccgaagag tgcgttcggc cgtggccagc gcatcaggat
     3181 cggaaacgta agtcgcgggc gtgatgagtc caacggtgtc gccgggcttc agcgctttcg
     3241 gccggatagc ttcgcctgtt ttctggagcg gggcgaaggc cccgggcatc gccataaata
     3301 cacgccgatt catggagcgg cctcaaatcc tcaaatcgta tccacgcgca cgcatcgcct
     3361 cgatgatgcc gttccggacg ctgctgactt cgtcggcgga aagtgtacga tccgcggcgg
     3421 cgagagtcat tcggtaagaa acactctttt gaccggcggc gagcggcggg ccggagtatt
     3481 ggcggacgaa ctccagagct tccagattgg gaccggcaaa ctgggccagc agttccccca
     3541 cgcgtcccac ttcatcccga aggccaatga taacggagag atcgaaagcg ctcgatggga
     3601 agcggcgtat gggtttgtac atggcgacgc cgggctggag cttatacata agctgcaagt
     3661 tcacatcgag aatggctgcg cggcccgcca ccatcgaagg atgcaattcg aacaaccgcc
     3721 ccacggtgtt cccgtgccat cggagcgcca cgctgcgatg cgggtgctca taaggacgcg
     3781 gttcgcagtg cgccacacca acacccggca tcaaacactc ggccaggcgc ttcaattcga
     3841 agagtccgtc cacaccgtct ccgcggccgt ataccaccgc cgccaggtga ggaatttcgt
     3901 cgggcagatt ggaggcgtcc tcgcgtttat gaatttccaa tccgatctcg aacagccgga
     3961 aagagtcgta gttcctgctg ttctcttcga tattcttcca aataccaggc agcaggctgc
     4021 atcgcatcaa tcgctgatca gagttgatcg ggttggcaat ttcgaggtgc gcattcgggt
     4081 ccaaatgaaa agcgcgcgcc atctccccgc tgataaacga gtagttatag acctcggtgt
     4141 agccttgcgc cgcagccatc tcccgcaccc ggtgatgaaa gtgtctctct tcattcgccg
     4201 gcggcacggt tgccgtcacc aggggagccg tgggagtgat ggtgtcgtat cccaccatgc
     4261 gcccgatctc ttccacgagg tcatccttga tcgagatgtc tcgcgtggcc cgccaacccg
     4321 gcactgtcac cgatagaacg ccgggcgagg atgcttccac cccgaattgc aggcgctcca
     4381 gaatgtctcg aacttcctct tcggaaatcg cgagcccaag ctttctcacc agccaatcga
     4441 gcggcagcaa aatcgccggc ggtggtatga tcacccgcgc ctggtcggca agcccgccaa
     4501 tgaggcgtat gccggggcag acttcgttca gcagttcaat cgcgcgagcc agcccgcgca
     4561 cggtgttcgc cgggtcctgc gacttttcaa agcgcatcga agcgtccgta cgaagcttca
     4621 gccccgccga agtcttgcga atacttaccg cctggaaatt cgcgctttca aggacgattc
     4681 gcgtggtggc gccgctgatt gcgctttcca gccctccgat aactccgccc agcgcaatcg
     4741 cgcccttggc gtcggcgatg acaagattcc cggcggacaa ctcgtaccgc tcctcgttca
     4801 gcgcggcgag gcattcgccc acgtgcgccc gccgcacgaa aatggtatcg ccgtgaagct
     4861 tatcggcatc gaacgcgtgc atgggctgtg cgatctccgc catcacatag ttcgtcacgt
     4921 cgacaatgtt gttgatggga ttcaggccaa tggcctccag gcggcactgc aaccatgcgg
     4981 gcgacggctg aacggagacg ttctcgaaaa ccagggcgct gtatcgcggg cacaattcga
     5041 agtcttcgat ggagattcgc gccggagagg agccggccgg caccagatcc actcgcacgg
     5101 gatcgaggaa aggcttcctg gtgatcgccg ccacttcccg cgccataccg gcatggcccc
     5161 aaagatccgg ccggtgcgtg atcgacttat tatcgatttc aataatgcta tccggcgtgc
     5221 atcccggctg gctgccttga gatggctcaa tgccttcgca ttcagcggtt ttgatggtga
     5281 tcaactgctc caggtgtttg gggtcgatat cgagaccgtc aacaagttcg cgaatccagt
     5341 tgtaggagaa tttcattctt gtccagcgtt aaaactgagt caggaaacga aggtcgccgc
     5401 tttggagcag ccgtatatcg tcgatgccgt agcgcatcat ggcgaggcgc gtcaaaccca
     5461 aaccgaaagc gaagccgttc cactcctccg gatcgattcc gctcatacgg agcacgttcg
     5521 gatgcaccaa accgcatgga agcagttcga cccaaccgga ctgcttgcaa accgggcatc
     5581 cgcttccacc gcagatgagg cacttgatgt ccagctcgaa gccgggttcg acgaatggaa
     5641 aataacccgg gcgcaaccgt acttcgacat cgcgatggaa aatggcgctc agcaacgtct
     5701 tcatgaagaa gatgaggtgt gccacggata catcgcggtc gatcatcatg ccttcaagct
     5761 ggtagaacgt gtgctcatgc gaggcgtcaa cctcctcgtt gcgaaacacg cgtcccggcg
     5821 cgatcatgcg caaaggcgga cccatccgtt ccatgccgcg aacctggacg ggcgaggtat
     5881 gcgtgcgcag caggtgcccg tccgtcagcc aaaaggtatc ctgcatgtcc cgcgcgggat
     5941 gatgcggcgg gatattcagc gcatcgaaat tgttgtactc cgtttccact tccggtccgt
     6001 cgagaacggc gaagcccaat gaaataaaga gatcctcgat ttcggtctgg atgcgggcga
     6061 tgggatgcag ggcgccgggc cgggttccgg gtgcaggcag cgtcaagtcc acccattccg
     6121 attccaggcg ctctcgcaac gcttcctgat cgaactggcc cttctttgaa tcgagctccg
     6181 tctccaggtc ctgcttgacc gaattgagca gttttccgat tcgcgcccgc tcttcgggag
     6241 caagttttcc aagttctttg ctgatctgag ccagggtccc cttgcggccg agcacttcaa
     6301 tccgcacggc ttccagagat tgaggcgctt cggcggcagc gatacgctcg cgcgcgctac
     6361 actccaggtc gcgtaaagtc gattccagca tgggcaggtg aaatttctat tgtacgcacg
     6421 ctcgccgggc ttgcgcctgc aacgattttt cggcccatga gcatacgagt tcgctataat
     6481 ttcccttcta cactcattcc gctggagcaa aggaatcctt atgcgaaaga aagttactgt
     6541 cgtcggagcg ggaaacgtgg gcgcgaattg cgctctgcgg atcgccggca aagaactcgc
     6601 cgatgttgtg ctggttgatg tggtggaagg agttccacag ggcaaggggc tcgatatcct
     6661 tcagtccgga cccgtcgagg gctacgatgt ctccatcacc ggcgccaacg attacgagcc
     6721 gacggccaat tcggatatcg tcgttatcac cgccggcttt ccgcgaaaac ccggaatgag
     6781 ccgcgacgat ttgcttctgg ctaattacga ggtagtgaaa acggccacgg aacaagccat
     6841 caaatattct cccgattcca tcctcatcct ggttaccaat ccgctggatg cgatgtgctg
     6901 gaccgcacag caggtctcgc ggttctccaa gaaccgtgtc atcggcatgg ccggcgttct
     6961 cgataccgct cgttttcgga ccttcatctc acaggagctt gatatctcgg tcgagaacat
     7021 caccgcgctg gtgctcggcg gccacggcga caccatggtg ccactcgtgc gcctggtgac
     7081 cgccggcggc attcccctga ccgaactgct ggaccaggca gccatcgaca ggcttgttca
     7141 acgcacacgc gacggcgggg cggagattgt acggtatctc aaaaccggca gtgcttacta
     7201 cgcaccttcc gccgcgacgg tggagatggt ggaatcgatt ctgaaggaca aaaaaaaggt
     7261 actgccctgc gctgcgctgc tggaaggcga atacggcatt tcagggctgt ttgtgggtgt
     7321 gccggtgaaa ctgggcgccc gcggaatcga aaagatctac gagttgaagt tgaccgctga
     7381 agagaaagcc atgctcgaca agagcgccgc tgccgtgcag gaactggtgg acgtgatcaa
     7441 gcagaagatg ggctgatccc gaaacgccca agggagccat ggctgaacag atacggccgt
     7501 acatcgaccc gaagtacgcc gttttccggc tgagagtggc gaagtccgga attcatcgct
     7561 ggggtgtttt cgccgaagag cggattcctg cgcgccgcaa aataatcgag tacaccggag
     7621 aacggatcag ccggatcgaa accaaacgcc gcagctcatc tcgcgaatac gtctacttat
     7681 tcaccctgaa tagctattgg tgcgtcgatg gagcggttgg cggcagcggt gccgaactga
     7741 tcaaccattc ctgcgacccg aatttgtact cctgcattat caaagaccac atcctctata
     7801 tgagcaagcg ggtaatcgag ccgggggagg aactgaccgt cgattaccat ttcgcgaaag
     7861 acatcgagac ctacatttgc cgctgcggcg catcgggctg ccgcggcacc atcaacgtga
     7921 aataaaacgc agttcgcggc caggataggg aaggcgccag cgggcttcga tttcaagaag
     7981 ccggttgtac ttggccaatc gctccgagcg tgtgatggag ccaatcttaa tttgtccgga
     8041 agcgctggcg accgcgagat ccgccagaaa ggagtcctcg gtctccccgg aacgcgccga
     8101 aactaccgcg gtccaattgg cagccaaggc ttcgtccaat acttcaaaag tttcagataa
     8161 ggtgcctatt tgattcatct taactaaaac agaatttgcc gctttttgct gaatcgcctc
     8221 tcgcacgcgc gcggggttcg tcgtaagaaa atcgtcaccg atgacctgcc gcccgccgcc
     8281 aagcgccgcc gtcagttccg gccagtttcc ccaatcgtct tcgtgaagcg ggtcttccag
     8341 ggacacgacg ggataacgca cgcaccagtc ccgcaacagg gcaatcatgc cggctgctgt
     8401 cagcctacgg ccctcggttt tgaggtggta ttcgccttcg gagaagaaat ggctggaggc
     8461 gacatcgatg gcaatcgaga cctgttcgcg gggctcgaac cccgcgctcc cgatggcctc
     8521 cgtcagaagc tggagcgctt cttcgttggt cgaaagcagc ggaccccaac cgccttcgtc
     8581 tgccactccc gtcagtgtca gacctctggc gcggcacagc aggaatacct gccggtgaat
     8641 cgcgaccgaa gcctccagcg cctccgccag gttgtcgaag ccgtgcggaa tgatcagaaa
     8701 atcctggaat tcgatgttgt ggccggcatg ccatccgccg gagagaatat tgatcatcgg
     8761 caccgggagc acggcagttc tcgcacccgc gagagattgc caaagaggaa ccttacgggc
     8821 ttgcgctacc gcccgcgcga ccgcgcaaga gacccccagg atggcattag cacccaaacg
     8881 cgatttgttg ctggtgccgt ccagttcgat catcttccgg tcgagggctg cccggtcttc
     8941 cgcatcggcg ccttcgaggg cggctgacag gatatcgttg acgttggcgg cggccttgcg
     9001 aactcctttg ccggcgtagc gagcgtcgcc gtcccgtaac tcgacagcct cgtgacgtcc
     9061 cgtggaggcg cccgaaggca cctgcgccgg cgcggttgtg ccgtcggaaa gctgcgcttc
     9121 cacggcaaga gtgggattgc cgcgcgagtc gaggatctct agtgctctga gctgcctgat
     9181 ggtgagagtc attccggtat gcggtcaaaa agctcttcgg tgctcgaagg cagatcggct
     9241 accagtccca gtccgaacgc gcgcacgcgg gccttcgccg cttcctcctg tatgtattcg
     9301 gcgggcgatt tgccgtcgat gcgcgcaagc agaagcccgg cgaggtgctc gatcgtatcc
     9361 tgctcgaatt cctccggcag ttcgcgaaca gtgtccatca ccgcgtcgcg aaaagcaagg
     9421 gccgccgagc ggtacccgga gcgccaatac ggccggtgga aggtcttcag gaccaggtgg
     9481 ttcgtcagaa acgccgcatc gaacgccggg tcgccatagt gcaccacttc gaaatcgatc
     9541 gccatcaccg atccgccatc caccaggaag tttttggggc tccaatcgcc gtgcaccagg
     9601 gaaatgcggc gggtgcgcgc tttctgaatg aggcgttcga agaacagggc cagtccggga
     9661 tggcgttggg ctgtcgcgcg atagtaggga tcgagccgca gttgatcgaa gacggtttgg
     9721 tcggagaacc tggctttcca atcgggccgc ttgcggctct cgccgataat gcagccgagc
     9781 atctggccga ttttggccgc aatgcccgta tcgatgttgc ccgccagcag aagcgatttc
     9841 caggtcgccg cgtgcgccgg agctgccgac atggcaaaga tgcagttcgc ctggtcttca
     9901 aagagaatac gggggaccga gtccggcgga agatgagggc cgagggcctc catggccgcg
     9961 cactcccggt gaatccgtgt gcggtccgag agccactcct gttccactcg cagtttctca
    10021 agggactgtt tcagaacaaa acgcttcgtt ccgcactcta ccagcagcac cgtgttcgat
    10081 acgccgccgc cgagctcgcg aatcccgatc gcacccgcgt ccgtcccgca atgagccttt
    10141 acatattcgt gagcggtcga caaagtcagt tcgaacaagt gccgatgata gcaaaccgga
    10201 ctgccgggta cttcagcgcc ggcgcgcaaa tagcgatagc accttgccca gcgccgaact
    10261 ttttggctct tcatccggga gttcggaccc gcccgtcatt gccgctgaga gggcggcgag
    10321 actctttccg aatttccccc gcggaggaac cgttttgcct tccatcgtag ctttctgtat
    10381 ggcatcgtag tcgctcggca tggtgtggca aacctccagt ttcagtgcgg tttcgatggt
    10441 ctcgccgctg acacccagct cctcactgaa acgattgacg acgacgcgga tcctggagcg
    10501 atccacttta cgccgttcca ggtaggaaag agcgcgctgc ccggcctgga ttgcgggcaa
    10561 ttcgttggtc atcaccagca acacctcgtc cgcatgtgcc gcgagctgcc cgttccagtt
    10621 gccgtacact ccgctggtat ccagaacgac gatgtcatag caggatttcg cgaactctat
    10681 caggtgaccg ggatccggca ggtcgccgat accgcgagcc ggatcttcgg gcgacagcag
    10741 aacatcgagc ccattccacg atgcaaccaa cgccttccaa atatccacat cgaggctgtc
    10801 cccgcgttgg agagcgtcca tgaaactgta ggcggaggtc agcttcaacg caaatgagac
    10861 ggttcccgta agtggatcga ggtctgccag cagaatgcgc tttgagccat tgcgcttcca
    10921 ctgatgtgca aggtgaaaag ccaatgttgt ggcgccgcac cctcctttgg caggcattac
    10981 gcaatatact tttccgccgg cttgcgaaac ctggtaggtt tcgggacgca ttcttgccag
    11041 ccgctccatc acaggttcca gttcccccgc ggtgaaagga gcggtcagga agccggttgc
    11101 accttgacgc aaacagcgga gaatgaaatc cgaatctgtc gatgcgagta gaaccacaat
    11161 ttgcagcgtt ggatcctggc gcaacaagtc cgcgatcagt gcaagtgctg tgtccttgtt
    11221 cgtggaagcg tccagaaagc agggattgac ttgcctcccg gaaaagaatt cgccaagggc
    11281 ctcggccggc gggtacgttc gcatctccgt gaccggtgcc aaaccaggta cttgcggcat
    11341 taacggaaga aactgcccgg ccatcccgcc gtcggggcac accaataggg tgtgccagtt
    11401 ctccttcggc ctggctgtat ctgtaccggc catctcccgt tccaaggaca gcgacaacat
    11461 cctccagtca attcatcgg
//
LOCUS       DASTMK010000545         4091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_347618, whole genome shotgun sequence.
ACCESSION   DASTMK010000545 DASTMK010000000
VERSION     DASTMK010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347618"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFQ79_22560"
     CDS             <1..835
                     /locus_tag="VFQ79_22560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142522.1"
                     /translation="AISNTRLPPELGRPDLFLEASRAARNANRFGLGAILDAIGRVHR
                     VSAEDRVHALVHLAQIVPRYREEIESWLAVEMSARSKRWLEEMESLLFNGHNATVLTT
                     ILPPFYEALDVPERVARIERLQQRAIQVLIKDKQFITALELLQALPERQPKLEAACHE
                     GAGDFCRAAECHLAAGNTKEALSCYRSIPDLDAALQLLQNTGEHPAAASLQWMSRVRN
                     VLAERPGNFNKTVTAAEKKLLEEMLEKGLGVTRRKPAPRKAAKKTVAKKPPVPRKRTS
                     K"
     gene            942..>4091
                     /locus_tag="VFQ79_22565"
     CDS             942..>4091
                     /locus_tag="VFQ79_22565"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142523.1"
                     /translation="MPPQKALYTAVAPLVMLLLASVLQGQQTRGVILGRVTDSSGLAV
                     PNVEVTLLNEKTGLSSAARTGTTGEYTFVSLDPGSYAISASAQGFKKAVIQNVTLFVA
                     QTARVDVTLEVGEVATSVEVQATIPVVQSERSSVGSVVDGTQIVSMPLNGRASIYSLM
                     ALAPGVGRAAINPVIAGGSWVGAANMTVDGAANVDVGNERVMPLAPSLESIAEFSVIG
                     NGASAEYGRGGAQIIAATRAGTNEFHGSLFAFNRNRVTAAKNFFATGLPKPKFNRNEF
                     GGSLGGPVVRNKLFFFGVYEGLRQRVSSTSTSAMPTVALKRGDFSGLPAIRDPFAGGA
                     PFPNNQIPAERISSASAELMKFTSDPNTPGTGAAGLGNNFIVNAPTREFNDRYTARVD
                     YQITPKNNFTARYFQVDNGPYEVTGNAGKFGGWDGFGTATKNAMGSYTRVLSPRLIAE
                     SRFGFAHTRYFRTPQNSSFDPSTIIPGLISPAEGLGGLPTVNITGFRGFNDQPGSGDR
                     QRNWEFFQNVTATLSKHTIKGGFEFQRASSLNWQNPPPYRGQFNFDGRYTGHPFSDFL
                     LGTSSLTGRVSKNVIAEPQNSRWAGFVQDDWNVSRTLTLNLGLRYEYAGLFANSQGDL
                     SNFYPDLGQLVVISGTPDPRLMAALPVVMGSDVGLDTGNWLNKDRNNWAPRLGFAWRP
                     LGTTKFVVRSSYGIYYNVVSGYSYFGFATNPPFRVSETFEPAAGSVPSLTFANAFPGA
                     GTVGGNPTLTTFSRDRRTPYHQQWNFTLEYELLPNTAVRASYIGNMGTHLERSFNLND
                     PPPAPGAVQPRRPYQPFGAITYVESGRSSILHGIQLSAIRRFASGFSFQLEYQYNKAL
                     GEQTYGLSPMDNRNTRLDRGDLDFIRRHAMRANYVYDLPFGRGKKFLNSVTGVADKLL
                     TGWQLTGIVGMGTGEPFSVTFTSTTLGWPSSRADIVGDPTPGGRSIAQWFNPAAFAAP
                     APFLYGNSARNMLFGPGYFDWDVGIFKNTALTERTTLEFRAEFFNFPNHANFGLPAGN
                     ISVPATVGRINSAGDPRTIQ"
BASE COUNT          822 a         1233 c         1221 g          815 t
ORIGIN      
        1 tgcgattagc aacactcgcc ttcctccgga attgggccgg ccggacctgt tcctcgaagc
       61 ttcgcgagcg gcacggaacg caaaccgctt cggactcggc gcgatcctgg atgcgatcgg
      121 gcgtgtgcat cgtgtgagcg ccgaggaccg ggttcatgcc ctggtgcacc tcgctcagat
      181 tgttcccaga tatcgggagg agatcgagtc ctggcttgcg gtagagatga gtgccaggag
      241 caagcgctgg ctggaggaga tggaaagctt gctcttcaac ggccacaacg cgaccgtctt
      301 gaccactatt ctgccgccgt tttatgaggc gctggatgtt cccgaacggg ttgcgcgtat
      361 cgaacggctt cagcaacgcg ccatccaggt gctcatcaaa gataagcaat tcataaccgc
      421 tctggaattg ttgcaggcgt tgccggaacg ccagccgaag ctggaggccg cctgccatga
      481 gggtgccggt gatttttgcc gcgccgccga gtgccatctg gcagcaggca acacaaaaga
      541 ggctctgagc tgctatcgct ccatccccga tttggacgca gccctccaac tgctccagaa
      601 caccggtgag cacccggcgg ctgcgtcact gcaatggatg tccagagtgc ggaacgtgtt
      661 ggcggagcgg cctggaaatt tcaacaaaac ggttacggcg gctgaaaaga agctgttgga
      721 agagatgttg gaaaaagggc tgggcgtcac cagacgtaag cccgcgccgc gtaaagcagc
      781 aaagaaaacg gtagcgaaaa agccgccggt tcctcgcaag cggacgtcca aatagccgct
      841 cccacgagca atttcctttt gacgctgtaa cgtggttgcg atacagtcgc atcaatacgg
      901 catcgtgccg tgaagactct gggctacgca aggagaaccc catgccgccg caaaaggctc
      961 tgtatacggc tgttgcgcca cttgtcatgc tactgctggc ttctgtttta cagggccagc
     1021 aaacccgcgg tgtcatcctg ggacgcgtca cggattcctc cggactggcg gttcccaacg
     1081 tagaagttac acttctgaac gaaaagacag gcctttcctc cgctgccagg acgggcacga
     1141 cgggcgagta caccttcgtg agtctcgatc ccggcagtta cgccatttcg gcgtccgcgc
     1201 agggattcaa aaaggccgtt atccaaaacg tcaccctttt tgtggcgcag accgcgcgtg
     1261 tagacgtcac actggaagtc ggtgaagtgg caaccagcgt agaggtgcag gccaccattc
     1321 cggttgtgca gtcggagaga tcgtcggtgg gtagcgtggt ggacggtact cagattgtct
     1381 cgatgcccct gaatggccgt gcgagcatct atagcctgat ggcgctggcg cccggagttg
     1441 ggcgggcagc catcaatccc gttattgccg gcggcagttg ggtgggcgcg gcgaacatga
     1501 ccgtggacgg cgctgcaaat gtcgatgtgg gcaacgagcg cgtgatgccg ctggcgccat
     1561 cgctcgaatc catcgcagag ttcagcgtta tcggcaatgg cgcatcggcg gagtacgggc
     1621 ggggaggcgc gcagatcatc gcggcaactc gcgcgggaac caacgagttt cacgggagcc
     1681 tgttcgcgtt caaccgcaac cgcgtgaccg ccgcgaagaa tttcttcgcc accggtctgc
     1741 cgaagccgaa gttcaaccgg aacgaattcg gcggttcgct gggcggacct gtggtccgga
     1801 acaagctgtt cttctttggt gtgtacgaag gtttgagaca gcgggtgagc agcaccagca
     1861 cgtccgccat gccgacggtg gcgttgaagc gtggtgactt ctccggtctt cctgctattc
     1921 gggacccttt tgcgggcggt gcaccgtttc caaacaacca gattccagcg gagcgcatca
     1981 gctccgcgtc cgccgaactg atgaaattca cctctgatcc gaatacccct gggacgggtg
     2041 cggccggtct gggcaacaac ttcatcgtca atgccccgac gcgagagttc aacgatcgtt
     2101 acacggcgcg cgtggactac cagatcacgc ctaagaacaa cttcacagcg cgctactttc
     2161 aggtggacaa cggaccttat gaggtaacgg gcaacgcggg caaattcggt ggatgggacg
     2221 gcttcggcac tgccaccaag aacgcgatgg gatcctatac gcgtgtgctt tcgccgcggc
     2281 tgattgcgga atcgcgcttc ggattcgcgc acacgcggta cttccgcact ccgcagaaca
     2341 gttcgttcga cccgagcacc atcattccgg ggctgatttc tccggcggaa ggtctgggcg
     2401 gcctgcccac cgtgaacatc acgggtttcc gcggcttcaa cgaccaaccc ggctcgggcg
     2461 accggcagcg caattgggag ttctttcaaa acgtaacggc gacgctcagc aagcacacca
     2521 tcaaaggcgg attcgaattt caacgggcat cctcgctcaa ctggcagaac ccaccgcctt
     2581 accgcgggca gttcaacttc gacggccgct atacggggca tccattctcc gactttctgc
     2641 tgggcacgtc atcgctcacc gggcgcgtga gcaagaacgt catcgccgag ccacagaaca
     2701 gccggtgggc cggtttcgtg caggacgact ggaacgtctc acgcacactg acgttgaacc
     2761 tgggtctgcg ttacgagtat gccggccttt tcgcgaacag ccagggcgat ttgtccaatt
     2821 tctacccgga ccttgggcaa ctggtggtga tctcgggcac tcccgacccg cgcctgatgg
     2881 cagccttgcc cgtcgtgatg ggttcggatg tgggattgga caccggcaac tggctcaaca
     2941 aggaccgcaa caactgggcg ccccgcctcg gcttcgcctg gcgcccgctg ggtaccacga
     3001 agtttgtggt gcggtcgagt tacggcatct attacaacgt ggtgtcgggc tacagctatt
     3061 tcggtttcgc gaccaacccg cctttccgcg tttcagagac tttcgagccg gcggcaggat
     3121 cggtgcccag cctcactttc gcgaatgcgt tccccggtgc gggtaccgtt ggcggaaacc
     3181 ctacgctgac gacgttttcg cgcgaccgca gaactcctta tcaccagcag tggaacttca
     3241 cgctcgaata tgagttgctg cccaacacgg ctgtgcgagc cagctacatc ggcaacatgg
     3301 gcacgcatct ggagcgatcc ttcaatctga acgacccgcc gccggcgcct ggagcggtac
     3361 agccgcgccg gccttatcag ccgttcggag caatcactta cgtggaatcc ggcaggagct
     3421 cgattctcca cggcatccag ctctcggcca tccggcgctt cgcttccggc ttctcgttcc
     3481 aactggaata ccagtacaac aaagcgctgg gtgaacaaac gtatggtctc tcgccgatgg
     3541 ataaccggaa cactcgcctt gaccgcggcg atctcgactt catccgccgt cacgccatgc
     3601 gagccaacta cgtttacgat ctgcctttcg gcagagggaa gaagtttctc aattctgtta
     3661 ccggcgtggc tgacaagctg ctcaccggtt ggcagcttac gggcatcgtc ggtatgggca
     3721 cgggtgaacc gttctcagtg acattcacca gcaccacatt gggctggccc agcagtaggg
     3781 cggatattgt cggcgatccc acgcctggcg gccggtccat tgcgcagtgg ttcaatccgg
     3841 cggcgttcgc ggcgccggca ccgttcctat atggcaactc cgctcgcaat atgctctttg
     3901 gtccaggcta cttcgattgg gatgtcggca ttttcaagaa caccgctctc accgagagaa
     3961 cgacgttgga gttccgggca gagttcttta atttccccaa tcacgcgaat ttcggtctgc
     4021 ccgccggcaa tatcagcgtc ccggctacag tcggacgcat caacagcgcg ggagacccac
     4081 gcacaatcca g
//
LOCUS       DASTMK010000546         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_29668, whole genome shotgun sequence.
ACCESSION   DASTMK010000546 DASTMK010000000
VERSION     DASTMK010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29668"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..477
                     /locus_tag="VFQ79_22570"
     CDS             127..477
                     /locus_tag="VFQ79_22570"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0142524.1"
                     /translation="MFDSPLPLETTPAEVQSRMRSGERIQLIDVREPAEHQVARIEDA
                     QLIPMGDVPSRLQAIEQAADDATTVVVFCHHGVRSLHVANWLRGQGVENVQSMQGGID
                     RWSLEIDPGIPRYT"
     gene            486..1709
                     /gene="dgoD"
                     /locus_tag="VFQ79_22575"
     CDS             486..1709
                     /gene="dgoD"
                     /locus_tag="VFQ79_22575"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEU0142525.1"
                     /translation="MKRRHFLAQSAVAPLLQAAPQAGGRPALKITDIKTFLVGAGGRN
                     LVFVKVETDQGIHGIGEAYSCGPDEATAATIADFKTWLVGQDPRNIEHIWATLYNFTR
                     FPGGLVVNAAMSGIEHALWDIAGKAAGLPVYMLLGGKVRERIRVYQSAGGGEPSQVAE
                     NAKRLVEKYGYTAVKMQPHPPKGNALPYNAVTRAAGQRVRAVREALGPDVDIGVDVHA
                     KFFEVSRAIRVAREIEPYHPMWLEEPIRPENPDAMAKLAAHVNIPLASGECNYTKFEF
                     RNILALQALDIIQPDICVCGGVMEMKKIAAMAEAHYVMVAPHNPMGPLATTVNVHFAA
                     STPNFLVLEYHPDDSAPRKDLLKEPLMVKDGYIPVPVKPGWGVELNEEAFRHYPAKRW
                     HRGFDYREDGSVAFI"
     gene            1742..3454
                     /locus_tag="VFQ79_22580"
     CDS             1742..3454
                     /locus_tag="VFQ79_22580"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0142526.1"
                     /translation="MRAIYHFLAISASVLLLQAADADLILHNGKIVTVDRNFSIRQAV
                     AIKDGRIAAAGTSEAILKSERGTQTRVIDLKGRTVLPGLIDAHVHPLGAGLSEFRAPL
                     PPLDSYAAVQSYIREQAKRTPKGQWIVVPRTFPTRLKEMRMPTREVLDVTKEHPVMFD
                     ASYVWVVNSKALEVSGIDRNTPNPPGGVIVKDEKGDPNGILKNAGALLKGLRRTETFS
                     EEERLQALEAMLKRYAAAGLTTIGDRAVSVDSVALYEKLRARGPLPVRAVLTWRLGSA
                     PPAEEVAAVIRKSNWTTNQGDGWLKFGTFKLTLDGGMTIGTAYQRYPYGVFGKQLYGQ
                     TDPDDRGQRFIAAEKLASVLDTARDKGWQLTAHSQGGGAVDALLDAFETLNRERPIAP
                     TRSHVMHASFQSPEAIARMKRLGVLADVQAAWLYLDGPALTKVFGNDGMRYFFPLRSY
                     VDAGIIVAGGSDHMIGFDKNSAVNPYNPFLGMWTAVTRKMKDGAVLHPEERISREEAL
                     KMYTIWAAYLQFDDEKKGAIEPGKLADLVVIDRDYMTCPEDEIKAVEPVATIIDGKIV
                     WERK"
     gene            complement(3468..4193)
                     /gene="aqpZ"
                     /locus_tag="VFQ79_22585"
     CDS             complement(3468..4193)
                     /gene="aqpZ"
                     /locus_tag="VFQ79_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEU0142527.1"
                     /translation="MSLQKRAFAECIGTFWLVFGGCGSAVLAAAFPDLGIGFHGVALA
                     FGLTVLTMAYAIGHISGCHLNPAVSVGLAVGRRFPASELGAYIGAQVIGAIAAAGVLY
                     VIASGKAGFTLAAGFASNGYAQHSPGGYSLMACLVAEVVLTCAFLLIIMGATDRRAPQ
                     GFAPIAIGLGLTLIHLIGIPVTNTSVNPARSTGPAIFVGGWALEQLWLFWVAPIVGAA
                     IGAVIYGAIAKEPAAVSARSARV"
     gene            complement(4421..5791)
                     /locus_tag="VFQ79_22590"
     CDS             complement(4421..5791)
                     /locus_tag="VFQ79_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0142528.1"
                     /translation="MNRRSITLLAIDDDPESLALVRAALKRDDLEILTTEDPEAGLDM
                     VRRLRPQIVLLDLIMPKLSGLEILGSIMSIDPATEVVLVTAHYSSETAVEAMQKGASD
                     YLDKPLQIDKLRERIDRLIADVRHRNRADELSEEMVKACRFEGIIGRSPLMLDVFARI
                     RRIAPHFRTALITGDTGTGKELVARAIHSLSPVAGGPFVVANCAAIPENLLESELFGH
                     VRGAFTGAVQDKAGLFEHANNGTLFLDEIGEMPLAAQPKLLRAVQNQELQRVGSLATR
                     KVNVRILAATNRDPRTSMKDGDFREDLFFRLSMVQIKLPSLEERKDDLPLLLHHFIQH
                     FAQTYNKPIVGISRRAEAALLRYRWPGNIRELENVIGYACMMTDSTKLDVQDLPDLFG
                     SASEPDASAPAPLVSLDEMQRIHARRVVDQLGGDKTRAAEILGVSRATLYRLLSPKVG
                     TAHFTE"
     gene            complement(5809..>6173)
                     /locus_tag="VFQ79_22595"
     CDS             complement(5809..>6173)
                     /locus_tag="VFQ79_22595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0142529.1"
                     /translation="LFRAMLQPTYAVRLYGDGEEALAAMAREVPDLVLLDISLPGMDG
                     IEVLRLMRADESLRSIPVVAVSAHAMAGDRERFLSEGFDEYVTKPISSRAALLAVVEP
                     LLTFGTGRAHRHQSGTAK"
BASE COUNT         1336 a         1885 c         1858 g         1094 t
ORIGIN      
        1 acgaaaagct ggcggcgaag tatccgttcg ggaccttcga aagcggcgaa cgcgggcggc
       61 tgaacggcgg atggggtgag attcgccgcc gcgacggaac catcatcaaa caataggaaa
      121 tacgaaatgt ttgatagtcc cttgccgctc gaaactactc cggccgaagt tcagagccgc
      181 atgcggtcgg gcgagcggat tcaactgatc gatgttcgtg aacccgccga gcaccaggtt
      241 gcgcggatcg aggatgcgca actcatcccg atgggcgacg taccttcgcg ccttcaggct
      301 atcgaacagg cggcggacga tgcaacaacc gtggtggttt tctgccacca cggcgtgcgc
      361 agcctgcacg tggccaactg gcttcgcggg cagggcgtgg aaaacgtcca gagcatgcag
      421 ggcggcatcg accgctggag cctggaaatc gacccaggca taccgcggta cacttaaccc
      481 tatctatgaa gcgccggcac tttctggcac agagcgcggt tgcgccgctg ctccaggccg
      541 ctccacaggc gggcggccgt cccgccctga agatcaccga tatcaaaacc tttctggtag
      601 gagcgggagg ccggaatctg gtctttgtaa aggtcgaaac cgaccagggc atccacggca
      661 ttggtgaagc ctattcctgc ggtcccgacg aggcaacagc ggctaccatc gccgacttca
      721 agacctggct ggtaggacag gacccgcgaa acatcgaaca tatctgggcc accctgtaca
      781 acttcacgcg cttccccggc gggctggtag tgaatgcagc gatgagcggc atcgagcacg
      841 cgttgtggga catcgccggg aaagcagccg gactgcccgt ttatatgctg ctgggcggga
      901 aggtccgcga gcgtatccgc gtgtaccaga gcgccggcgg cggcgagccc tcacaagtgg
      961 ccgaaaacgc gaaacggctg gtggaaaaat acggctacac agccgtgaag atgcagccgc
     1021 atccgccgaa aggaaatgct cttccctaca acgccgtgac cagggcggcc ggacagcggg
     1081 tgcgcgccgt gcgcgaagcg ctggggcccg acgtggacat cggcgtagac gtgcacgcca
     1141 agttcttcga ggtctcccgc gcgatccgcg tggcgcgcga gattgagccc tatcatccca
     1201 tgtggctgga ggagccgatt cgtccggaga atcccgacgc catggccaag ctcgcggcgc
     1261 atgtgaacat tccgctcgcg agcggcgaat gcaactacac gaagttcgag tttcgcaaca
     1321 tcctcgccct tcaggcgctc gacatcattc agccggacat ctgcgtgtgc ggcggcgtga
     1381 tggagatgaa gaagattgcg gcgatggcgg aagcccacta cgtgatggtg gcgccgcaca
     1441 accctatggg gccgctggcg actactgtca acgtgcactt cgcggccagc acaccgaact
     1501 ttctcgtgct cgaatatcac cccgacgaca gcgcgccgcg gaaagatctg ctcaaagagc
     1561 cgctcatggt gaaggacggc tacattcccg taccggtaaa accgggctgg ggcgtcgagc
     1621 tgaacgagga agccttccgc cactatccgg ctaaacgctg gcatcggggg ttcgattacc
     1681 gcgaagacgg ctcggtggcg tttatctgac cctgccggta caggaatgat agagttttca
     1741 tgtgagggca atctatcatt tcctggctat ttccgcgtcc gtgttgctgc tgcaggcggc
     1801 ggatgccgac ctgattctgc ataacggaaa aatcgttacc gtcgatcgaa atttttccat
     1861 tcgtcaggcg gtcgcaatca aggacggcag gatcgcagcc gccggaacca gcgaagcgat
     1921 tctcaagtcg gaacgtggaa ctcagacgcg tgtgatcgac ttgaaaggcc gcacggttct
     1981 acccggtctg atcgacgcgc acgttcatcc gctgggcgcg ggcctgagcg aattccgcgc
     2041 tccccttcct cccctcgact cctacgcggc cgttcaatcc tacatccggg agcaagcgaa
     2101 gcggacaccg aaagggcagt ggattgttgt gccccgcacg tttccaacgc gccttaaaga
     2161 gatgcgcatg cctacgcgcg aggtgctcga cgtcaccaaa gagcatcccg tcatgttcga
     2221 cgccagctac gtgtgggtgg tgaactcgaa ggcgctcgag gtcagtggca tcgaccggaa
     2281 cacgcccaac ccgcccggcg gcgtcatcgt gaaagacgaa aagggggatc cgaacggaat
     2341 tctgaaaaac gccggagcat tgctgaaggg cctgaggcgc acggagacgt tcagcgagga
     2401 agagaggctg caagcgctcg aagcgatgct caaacgttac gccgcggcgg gcctcaccac
     2461 catcggcgat cgtgctgtga gcgtggatag cgtggcactg tatgaaaagc tgcgcgcacg
     2521 cggcccgctt cccgttcgcg cggtactgac gtggcggctg ggctccgccc cgcccgcgga
     2581 ggaggtggcc gccgtgattc gcaagtcgaa ttggacaacc aatcaaggcg acggctggct
     2641 gaaattcggc accttcaaat taacgctgga tggcggcatg accatcggaa ccgcttacca
     2701 gcgatatccc tacggtgtgt tcgggaaaca gctctatggg caaacggacc cggacgatcg
     2761 cggccagcgc ttcatcgcag cggagaagct cgcctccgtt ctcgataccg cgcgcgataa
     2821 gggatggcag ttgaccgccc attcgcaagg cggcggcgcc gtggatgccc tgctcgatgc
     2881 cttcgagacg cttaaccgcg aaaggcccat cgcgccgacg cgctcacacg tgatgcacgc
     2941 cagctttcaa agtcccgaag ccattgcccg aatgaagcgc ctgggagtgc tggcggacgt
     3001 acaggcggca tggctctatc tggatgggcc ggccctcacg aaggtgttcg gcaatgatgg
     3061 catgcgttac ttctttccgc tgcggtcgta cgtagacgcc ggtatcatcg tcgcgggagg
     3121 cagcgaccac atgatcggct tcgacaaaaa ttcggcagtg aatccttaca acccgttcct
     3181 aggcatgtgg accgcggtca cacgcaagat gaaggacggg gccgtccttc atccggaaga
     3241 acggatctcg cgcgaagaag cgctcaaaat gtacaccatc tgggccgctt atctacagtt
     3301 cgacgacgaa aaaaaggggg ccatcgagcc gggcaaactc gccgatctgg tcgtcatcga
     3361 ccgggactat atgacatgcc ctgaagatga aatcaaagcc gtcgaaccgg tagccaccat
     3421 catcgatggc aagatcgtat gggagcggaa atagcgcccc ggatcgctca aactcgcgcg
     3481 gagcgtgcgc tcaccgccgc gggttccttc gcgatcgccc cgtatatcac ggctccgatg
     3541 gctgctccaa cgatcggcgc cacccaaaac agccaaagct gttcgagcgc ccagccgccc
     3601 acaaagatcg ccgggccggt gcttcgtgcc ggattgacgg aggtattcgt caccggaatg
     3661 ccgatcaggt ggatgagcgt aagccccagg ccgatggcga tgggcgcgaa gccttgcggc
     3721 gcgcgcctgt cggtcgcacc catgatgatg agcaggaatg cacacgtcag caccacctcg
     3781 gccaccagac aggccatcag ggaatagccg ccgggcgagt gctgagcgta tccattggaa
     3841 gcgaagccag ccgccagggt gaaccctgcc tttccgctgg caatgacata cagcacaccg
     3901 gccgcagcaa tggcgccgat gacctgagcg ccaatataag ctcccagctc ggaagctgga
     3961 aatcttcggc ctacagccag ccccaccgaa acagcggggt tcagatgaca ccctgaaatg
     4021 tgaccgatgg cataggccat ggtcagcacc gtaaggccga aagcgagggc cactccgtga
     4081 aagccaattc caagatcggg aaacgccgcc gcgagaaccg cgctgccgca gcctccgaag
     4141 acaagccaga acgtaccgat gcattcggca aacgctcgtt tttgtagtga catgctatcc
     4201 tccttttcgg aagcccacca atgcttttcc actccctggg cggtcgtggc tcggaaagct
     4261 gcacgagcga gccaacgctc agcaagcaag ccacgggccg catacgaatt tacacctttt
     4321 cccgccggtt atcacgatct caccaagccc gttcgtctca ggcgcggacg gaaatctcgc
     4381 ggcggagaca cattcgatgc ggggcttcgc ggtgaaaatc ctattcggtg aagtgcgccg
     4441 tccccacttt gggagacagc aaacggtata gggtcgcacg gctgacacca agaatttccg
     4501 cagcgcgggt cttgtcgccc ccgagctgat cgacgacacg ccgcgcgtgg atgcgctgca
     4561 tctcgtccaa cgaaaccaaa ggtgccggtg cggaggcatc cggctcggaa gcgctgccaa
     4621 acaggtcggg aagatcctgc acatccagct tggtcgaatc cgtcatcatg caggcgtagc
     4681 caatgacatt ctcgagttcc cggatatttc ccggccagcg atagcgcagc aaagccgcct
     4741 ccgcgcgccg gctgatccca acgatcggct tgttataggt ttgcgcgaaa tgctggatga
     4801 aatgatgcaa cagaagcggc aggtcatcct tgcgttcttc gagtgacgga agcttgatct
     4861 ggaccatgga aagccggaaa aacaggtctt cgcggaaatc cccgtccttc atcgacgtcc
     4921 gcgggtcccg gttggtggcc gcaaggattc ggacattcac tttgcgagtc gcgagggaac
     4981 cgaccctttg aagctcctgg ttctgcaccg cacgcaaaag tttgggctga gccgcgagcg
     5041 gcatctcgcc gatttcatcc agaaacagcg ttccgttatt cgcatgctcg aacagtcccg
     5101 ccttgtcctg gacggcaccg gtaaatgcgc ccctgacgtg tccgaacagt tcactctcta
     5161 aaaggttttc cggaatcgcc gcgcagttgg cgacaacaaa gggtccgccg gctacgggac
     5221 tgagcgagtg gatcgcccgc gccaccagtt ccttgccggt tccggtatcg cccgtgatca
     5281 gagcggtacg aaaatgcgga gcaatccggc ggattcgtgc gaacacgtcg agcatcagcg
     5341 ggctgcggcc gataatccct tcgaaccggc aggccttcac catctcctcg ctcagctcgt
     5401 ccgcccgatt gcggtgccgg acatctgcga tgagacgatc gatccgctcg cgcagcttgt
     5461 cgatctgtag cggcttgtcc aggtaatcgc tggcgccttt ctgcatcgcc tcgacagccg
     5521 tctcactgga gtaatgagcg gtaaccaaaa cgacttccgt ggcggggtcg atgctcatga
     5581 tgctgcccag gatctcgagc cccgacaact tgggcatgat cagatcgaga aggacaatct
     5641 gcgggcgcag gcggcggacc atgtcgagac cggcttccgg atcttctgta gtgaggatct
     5701 ccagatcgtc gcgcttcagc gcagcccgga caagcgcaag ggactccgga tcgtcgtcaa
     5761 tggcgagcag cgttatggag cggcggttca ctacctcatc atgatagttt acttggccgt
     5821 tccagattgg tgtctgtgag ccctgccggt accaaaggta agtaacggtt ctacaacagc
     5881 cagcagcgcc gcgcgggaag atatcggctt cgtaacatat tcatcgaatc cctcggaaag
     5941 aaatctttcg cgatctccgg ccatcgcatg ggccgaaacc gcaaccaccg ggatggaacg
     6001 caacgattcg tccgcacgca ttagccgcaa aacctctatc ccatccattc ccggcagaga
     6061 gatgtccagc aaaaccaaat cgggaacctc ccgcgccatg gcggccagag cctcttcccc
     6121 atcgccgtag agcctgacgg cgtacgtggg ctgcagcatc gcacggaaca agg
//
LOCUS       DASTMK010000547         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_636240, whole genome shotgun sequence.
ACCESSION   DASTMK010000547 DASTMK010000000
VERSION     DASTMK010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636240"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFQ79_22600"
     CDS             complement(<1..428)
                     /locus_tag="VFQ79_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEU0142530.1"
                     /translation="MISILWFGALLFAAYWPVLTRLFNQWMNDEDMGHGLFVPVIASY
                     IAWQRRDKVLAAAAKGGSWLGLVVVLYGALQLLVATLGAELFLARTAFIISLAGALML
                     LGGMATIRALAFPLFLLLFMVPIPAIIYNQITLPLQLFASS"
     gene            632..1267
                     /locus_tag="VFQ79_22605"
     CDS             632..1267
                     /locus_tag="VFQ79_22605"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0142531.1"
                     /translation="MKDLSLSGRIAQGLMKIGLAMKTNEWSESAKLQLSPTQAQILAA
                     LATRGPLRLSELAALLAVTLATASDAVSSLAKKGLVEKRTSEQDARALSIVPTRAGQK
                     TAKAVAAWPDFLATAVDTLSPDEQVAFHRGLVKMIGTLQERGQIPVARMCAHCTYFRP
                     NVHAGSRTPHHCDFIDSAFGDEFIRIDCGDFVAATTSEGWDAWVGRGSVTQ"
     gene            1292..1882
                     /locus_tag="VFQ79_22610"
     CDS             1292..1882
                     /locus_tag="VFQ79_22610"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEU0142532.1"
                     /translation="MSAQIPGYAYGQPRLAHSPVSLEDLDLLKQTVLFTDEDTHYLRM
                     SLDILKDQTGAILDVWYGFVASHPHLVYYFGKKQDGQPDGEYLAAVRKRFEQWILDTA
                     AANYDQKWLDYQHEIGRRHHRTAKNATDGVQSVDNISYRYLPALIYPVTATLKPFLAK
                     KGNTAGEVEGMHQAWVKSVLLQVILWSQPYIKDGDF"
     gene            complement(1907..>2512)
                     /locus_tag="VFQ79_22615"
     CDS             complement(1907..>2512)
                     /locus_tag="VFQ79_22615"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU0142533.1"
                     /translation="DENYPLRASSPYSASKAGSDLLALSYFTTYRLPVIVTRASNNYG
                     PYQFPEKLIPLMISNALEDRRLPVYGDGMQVRDWLYVEDHCRAIREVLKKGREGEIYN
                     IGGNCSLPNLEVIRRILAATHKPESLLETVKDRPGHDRRYALSSAKLMAETGWKPLTG
                     FDEGLARTIDWYRQNSGWVKRVKSGEYQNYYALNYGARGTV"
BASE COUNT          566 a          736 c          722 g          488 t
ORIGIN      
        1 gagctggcga ataactgtag cggcagcgtg atctggttgt aaatgatggc gggaatcgga
       61 accatgaaca gcagcagaaa cagcggaaag gcgagcgcac gaatagtagc catgcctccc
      121 agcagcatca gcgctcccgc cagcgaaatg atgaaggcgg tgcgcgccag gaagagttcg
      181 gcgcccagtg tggccaccag cagttgcagc gctccgtaca acaccacaac caggcccagc
      241 cagcttccac ccttcgccgc ggccgcaaga accttgtccc gccgctgcca ggcgatgtag
      301 gaggcgatga ccggcacaaa cagcccgtgc cccatatcct cgtcgttcat ccattggttg
      361 aacaggcgcg tcagcacagg ccagtaagcg gcaaatagca acgctccgaa ccataggatc
      421 gaaatcaaag taaaacggaa ccgggaatcg ggagcacttg ctaactgggg agaggtctca
      481 gtggaggcca tcgcttactt tcttcaagta caccacacgg aaccaatacc cacaacagga
      541 ctaaaggact ggaacttgta aacgcttttc cggtacacgc cccttgacag gccggcaggg
      601 cagccttatt attgattagg actcctaata aatgaaagat ctctcccttt ccgggcgtat
      661 cgctcaaggc ctgatgaaga ttggactcgc tatgaagaca aacgagtgga gtgagtccgc
      721 gaagcttcaa ctttcgccca cgcaagccca aattctagcc gcgctggcaa cccggggtcc
      781 tctacggctg tcggagctgg cagcactcct tgcggttaca ctggcgacgg caagcgacgc
      841 cgtgtcgagc ctggcgaaaa aggggctcgt cgaaaagcgg acgtccgagc aggacgctcg
      901 tgcgctttcg attgtgccaa cccgcgccgg gcagaaaacc gcgaaggcag tggcggcgtg
      961 gcccgatttt ctggctaccg ccgtcgacac cctttctccc gatgagcaag tcgcgtttca
     1021 ccggggtctg gtgaagatga tcgggacgct acaggaacgc ggccagatcc cggtagcccg
     1081 catgtgtgcg cactgcacgt acttccggcc gaacgtccat gccggcagcc gcacgccgca
     1141 ccattgcgat tttatcgact ccgctttcgg cgacgaattc attcggatcg attgtggtga
     1201 tttcgttgca gcgacaacct ccgagggttg ggacgcctgg gtaggtagag gttccgtaac
     1261 gcaataagcc atctacgaaa ggaaaaaagc tatgtccgca caaattccgg gatacgccta
     1321 tggccaaccg cggttggcgc attcaccggt gagcctcgaa gatctcgatc tgctcaaaca
     1381 gacggttctc ttcaccgacg aagacacgca ctatctgcga atgtcgctcg acatcctcaa
     1441 agatcagacg ggcgccatcc tcgatgtttg gtatgggttc gtcgcgtcgc atccccacct
     1501 ggtctactac ttcggaaaga agcaggacgg ccagcccgac ggggaatacc tggcggctgt
     1561 ccggaaacgt tttgagcaat ggattctgga caccgcggcc gccaactacg atcagaagtg
     1621 gctcgattac cagcatgaaa tcggaaggcg gcatcatcgc acggctaaga acgcgaccga
     1681 tggcgtgcag tcggtagaca acatcagcta tcgctatctg ccggcgctga tctatccggt
     1741 tacggccact ctgaagccgt tcctggcgaa gaaaggcaat accgccggcg aggtcgaggg
     1801 gatgcaccag gcctgggtga agtccgtcct tctgcaagtc atactgtgga gccagcccta
     1861 tatcaaagac ggcgatttct gagcggcagc ccggcgccgg ggccgttcaa acggtcccgc
     1921 gtgcaccata gttgagggcg tagtagttct gatattcgcc cgacttcacc cgcttcaccc
     1981 agccggagtt ctgccgatac cagtcgatgg tgcgcgccag tccttcatca aaaccggtaa
     2041 gcggcttcca tcctgtttcg gccatcagct tggcgctcga gagggcatac cgccggtcgt
     2101 gtccgggacg gtccttcaca gtctccagca ggctttccgg tttgtgtgtc gccgccagga
     2161 tgcggcggat cacttccaga ttcggcagcg aacaatttcc gccgatattg taaatttcgc
     2221 cttcacggcc cttcttcaaa acttctcgaa tcgcgcggca gtggtcctct acataaagcc
     2281 agtcgcgcac ctgcatgccg tctccgtata cgggcaggcg acgatcctcc agggcattcg
     2341 agatcatcag gggaatgagc ttttcgggaa actggtacgg gccgtagtta ttggatgcgc
     2401 gagtgacgat tacgggcagt ctgtaagtgg tgaagtaaga cagcgccagc agatcggacc
     2461 cggctttcga ggccgaataa gggctgctgg ctcttaacgg gtaattctcg tc
//
LOCUS       DASTMK010000548         6756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_636541, whole genome shotgun sequence.
ACCESSION   DASTMK010000548 DASTMK010000000
VERSION     DASTMK010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636541"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFQ79_22620"
     CDS             complement(<1..1230)
                     /locus_tag="VFQ79_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU0142534.1"
                     /translation="MNTLLLIGLVFAAARSGSNADVRFTDVTREAGIQFTHNSGRAGN
                     KWLPETMGSGCAFFDANQDGWLDIFVVNSKDWTARGRRSLPALYLNRGNGKFVDATRG
                     SGLDVELYGMGVAIGDYDNDGRDDLYLTALEGDRLYRNLGGGKFGDVTETAGIRNANF
                     GTSAAWVDYDRDGRLDLFVANYVKWTPKTDLWCSLDGATKSYCTPESYKGTSSKLYRN
                     LGGGKFEDASARAGIADPHSKSLGVAVFDYNSDGWPDLFVANDTQPNKLYRNNRDGTF
                     TEEGLSAGVAFGEDGVARGAMGVDAVDYDRSGRPHLLVGNFSNQMLGLYRNEGKGLFV
                     DEAPRSTVGRASLLSLAFGVFFFDYNLDGYQDIFAANGHIDEEIGRVQPKVQFKQLPH
                     LFRNLGKGSFREVTSTTG"
     gene            complement(1227..3965)
                     /locus_tag="VFQ79_22625"
     CDS             complement(1227..3965)
                     /locus_tag="VFQ79_22625"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142535.1"
                     /translation="MKRLWTAAVVLFAINTAYVAAFATPSIFYMANVVLHVFLGLVLS
                     VVLAWRLVRNRALRTLIPTSSQVLFAAAVFAGVYLLARGSLREHRWALYAHVITGVAG
                     VMMLAAVRAWRPKVAGALALLALLTLATYSYGRLFPNPHDRIRNPKIVPASMQEEGAG
                     PQSPFWPSSADTNTKGLIPSHFFLDSKLCGECHKDIYDQWQSSVHHFASFNNQFYRKS
                     IEYMQDVAGTKPTKWCAGCHDHAVFFNGRFEKPIKDQIDTPEAQAGLGCVSCHMITRV
                     DGSMGNGGFTIEYPSLHELAASKNPYLRAIDKFLTYLDPEPHRRTFMKPFMRLDASEY
                     CSTCHKVHLDVPVNDYRWVRGFNDYDNWQASGVSGQGARSFYYPDQPSNCVDCHMPLV
                     PSRDPGHHDGKVHSHRFPAANMAVAYVNRDAEQMRVTEEFLKSGFISVDIFAASPVDR
                     SPGQLQMRRRSGSPPEIMSTFAIGEEAEQSGEVLIREVGKVAAPLDEAGVRFEPGSDV
                     RVDVVVRTRKIGHFFPGGTVDAFDVWLELTADGADGKRIFWSGMVEDGGRGPVEKGAH
                     FYRAYLLDGAGNAINKRNAFQARSVLYVRLIPPGAADVAHFRLKIPATARGPIKLHAK
                     LNYRKFAHFYTQFAYAGTPGEGSVGLDHDNRPFTFFPTNIPKNVSGQIRGEIPDLHTV
                     TMAEAKSELAVARRGEATEWKPVVRRQDRERWNDWGIGMLLQGDLKGAEYAFRKVTEA
                     EPEYADGWLNVARALIQEGETDAAKPFLENAMAVNAKLGRIHFFKALVQKADGDYDSA
                     LASLRTVSAQYPRDRVVLNQIGRILFLQRKYAEAVGALERVLEIDPEDVQAHYNLMLA
                     YKGLGETEKSARAEKLFRRFKADESAQAITAKPRMLSPEDNNERQMIHDHESAVFQ"
     gene            complement(3962..>6756)
                     /locus_tag="VFQ79_22630"
     CDS             complement(3962..>6756)
                     /locus_tag="VFQ79_22630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEU0142536.1"
                     /translation="NYGLALLRAGKTAEGVAELERVQQQDPTLPHTWFNLGIEYKRRG
                     EAEQAIAQFEQMVKLVPDDAISHYNLAASYRIPGRDAEAIKHFEIAAKLEPNLAGPHF
                     QLFNAYRAAGRPADAKRELEIFQKIKKEQEGAAIPEDMEWNAYAEVYETIDGAAAAQP
                     DAKYVTRNLAGKVDAGTAGMALIDADGDGGTDLLVWSGQGLALYRNGSALVANAFPAG
                     PVISAAPGDFNNDGLSDVAVLTPSGVVLYRNQKGKFILQEARLPEGVFNKAVWLDYDH
                     DYDLDLVLLGKESRVLRNQGVAGFADRTADFPFVQGETLDGVVFRLVPDSKSMDLAVS
                     YRERKGVLYRDQLGGVYKAEPLDTLAEGARGLDAVDRNRDGRFEVIAASGAPSVTADF
                     DRDGDFDVVSVTPGGAIQRRDNQAGGGSWIAVRLEGVKNLKLAHYAEVEVKAGRVYGK
                     QIYTGVPLLFHLGPYKEADTVRIMWPNGLIQNEIRQPAGRMHLYKEKPRLSGSCPMIW
                     SWNGSEFTFITDVLGVAPLGASAGDGSYFPVDHDEYIQIPRDAIAERDGAYEIRITEE
                     LSEVAYLDKVSLIAIDHAADIEIFTNDKFKGPPFPEFRLFGIRNRMVPLRARQNGFDV
                     TSSVLRRDRIYAERFRRKMNGVAEKWALELDFGAGAARDNRALLVLNGWVDWADGSTF
                     LAEAQEGRGGLITPYLEVRDAQGKWQTVVEDMGMPAGKPKTIVVDLTGKFLSSSREIR
                     IVTNLAVYWDEIFLSEDTAPPQTKVTALPPDADLSFRGFSRVVIHPQRRQPEHFLYPD
                     PLPVSMWNQTPGLYTRYGAVGPLLKEGDDRFVIMGSGDELRLRFDARRLPALPSGFSR
                     DFLLLVEGWAKDRDANTAFSQAVEPLPFRRMSAYPYPPAERYPDTPVHSAYRREYNVR
                     PARQLLRPLGSGGQPR"
BASE COUNT         1328 a         2184 c         1894 g         1350 t
ORIGIN      
        1 gcccgtggtg cttgtaacct cccggaaaga ccccttcccc aaattgcgga acaagtgcgg
       61 cagttgcttg aactgcacct tcggctgcac gcgcccgatc tcctcgtcga tgtgcccatt
      121 ggccgcaaaa atgtcctggt agccgtccag gttgtagtcg aaaaagaaca ctccgaaggc
      181 aaggctcaac agactggcgc gccctaccgt ggagcgcggc gcctcgtcga cgaacagtcc
      241 cttgccttca ttgcggtaca gcccgagcat ttgattggaa aaatttccaa ccagcagatg
      301 cggccgcccg gagcggtcgt aatccacggc atccaccccc attgcgcccc tcgctacgcc
      361 atcttcgccg aacgcgaccc ccgcggaaag accttcttcc gtgaatgtgc catcgcggtt
      421 attccggtat agcttattgg gttgcgtgtc gttggcgacg aacaggtcgg gccagccgtc
      481 cgaattgtaa tcgaacaccg cgacgcctag tgatttgctg tgcggatcgg cgatgcccgc
      541 gcgcgcactc gcgtcttcga acttgccgcc gcccaggttg cggtaaagct tcgaagaagt
      601 tcctttgtat gactccggcg tgcagtagga tttcgtcgcg ccgtcgagcg agcaccacaa
      661 atcggtcttc ggcgtccact tgacgtagtt ggcaacgaaa agatccaggc gcccatcgcg
      721 gtcgtaatcc acccacgcgg cgctggtccc gaagttcgcg ttacgaatgc ccgccgtttc
      781 agtgacatca ccgaacttgc cgccgcccaa attgcgatac agccggtcgc cttcgagagc
      841 agtgaggtac aggtcgtcgc ggccatcgtt gtcgtaatcg ccgatcgcca cacccatgcc
      901 gtacagttcc acatcgagtc cgctgcccct ggtggcatcc acaaatttgc cattgccgcg
      961 gttcaaataa agggccggga gggaacgcct tccgcgcgcc gtccaatctt tgctgttcac
     1021 aacaaagata tccagccagc cgtcctgatt cgcatcgaaa aatgcgcagc ccgaacccat
     1081 ggtctccggc agccacttgt tgcccgcgcg gccgctgtta tgtgtgaatt gaatgcccgc
     1141 ttcgcgcgtc acatcggtga agcgtacgtc cgcattcgat ccggaccttg ccgccgcgaa
     1201 caccaggccg atcaacagca gcgtgttcat tggaagaccg ccgattcgtg atcgtggatc
     1261 atctgtcttt cgttgttgtc ttccggactc agcatccggg gcttcgccgt gatcgcctgc
     1321 gccgactcgt ccgctttgaa acgccggaag agcttttccg cgcgggcgga cttttctgtc
     1381 tccccaaggc ctttgtacgc cagcatcagg ttatagtgcg cctgcacgtc ctcgggatcg
     1441 atctcgagca cacgctcgag cgcgccgacc gcttcggcgt atttgcgctg caaaaacaga
     1501 atgcggccta tttgattcag cactacccgg tcgcgcggat actgcgccga taccgtccgc
     1561 aggctcgcca gcgcactgtc atagtctcca tccgccttct gcaccagcgc cttgaagaaa
     1621 tggatacgcc ccagcttcgc attcaccgcc attgcatttt cgaggaaggg cttagcggca
     1681 tccgtctcgc cctcctggat gagcgcgcgc gcgacgttca gccagccgtc ggcatattcc
     1741 ggttccgctt cggtgacctt gcgaaacgca tactccgcgc ccttcaaatc gccttgcagc
     1801 agcatgccga taccccaatc gttccagcgc tcacggtcct gcctgcgcac gacgggcttc
     1861 cactccgtcg cctctccccg ccgggcgacc gccagttcgc ttttcgcttc cgccatggtt
     1921 accgtgtgca ggtcagggat ctcgccccgg atctggccgg aaacattttt cggaatgttt
     1981 gtggggaaga aagtaaacgg ccggttatcg tgatcgagcc ccacggaccc ttctcccgga
     2041 gtcccggcat aggcgaactg cgtgtagaag tgagcgaatt tacggtagtt cagcttcgcg
     2101 tgcagcttaa tgggcccgcg cgcggtggcg ggaatcttca accggaaatg cgcgacatcc
     2161 gcggcgcccg gaggaatcaa cctgacgtac agtacactcc gggcctgaaa cgcgttgcgc
     2221 ttgttgatcg cgtttcccgc tccgtccaga aggtaggcgc gatagaaatg agcgcctttt
     2281 tccacgggtc cacggccgcc gtcttccacc atgccgctcc agaagatccg cttgccatcc
     2341 gcgccgtccg ccgtcaactc gagccataca tcgaaggcgt cgaccgtgcc gcccgggaag
     2401 aagtgaccga ttttgcgcgt gcgcactacc acgtccacgc gcacgtccga cccgggctcg
     2461 aagcgtacgc cggcctcatc gagaggagcg gcgaccttgc caacctcgcg aatcagaacc
     2521 tcgccactct gttcggcttc ctcgccgatg gcgaacgtcg acatgatctc cggcggcgaa
     2581 ccggagcgcc gccgcatctg caactgcccg ggggagcggt ccaccggcga ggcggcgaag
     2641 atatcgacgc tgatgaaccc agacttcaaa aattcctcgg tcacgcgcat ctgttccgcg
     2701 tcgcggttca cgtatgccac ggccatgttt gccgcgggaa aacgatggga gtggaccttg
     2761 ccgtcgtgat ggcccgggtc ccgcgaaggc accagcggca tatgacaatc gacacagttt
     2821 gaaggctggt ccggataata aaacgaacgg gccccctggc ccgaaactcc actcgcctgc
     2881 cagttgtcgt agtcgttgaa acccctcacc caccggtaat cgttcaccgg cacgtcgagg
     2941 tgcaccttgt gacacgtcga gcaatactcc gaggcatcca gccgcatgaa gggtttcata
     3001 aacgtgcggc ggtgcggttc gggatcgaga tatgtcagaa acttgtcgat ggcgcgcagg
     3061 taagggttct tgctcgccgc cagttcatgc aggcttggat actcgatggt aaagccgccg
     3121 ttgcccatcg agccgtccac tcttgtaatc atgtggcacg acacgcaccc gagcccagcc
     3181 tgcgcttccg gcgtgtcgat ctggtccttg atcggtttct caaaccgtcc attgaagaac
     3241 acggcatgat cgtggcaacc cgcgcaccac ttggtgggct ttgtaccggc tacgtcctgc
     3301 atatattcga tcgacttccg atagaactgg ttgttgaaag acgcgaaatg atgcactgag
     3361 ctttgccact ggtcgtaaat atccttgtga cactcgccgc aaagtttgga gtccagaaaa
     3421 aagtgggacg gaatcaggcc cttggtattc gtgtcggcgg aggatggcca gaatggcgat
     3481 tgcggaccag ccccttcttc ctgcatcgaa gcaggcacaa tcttcggatt gcgaatgcga
     3541 tcgtgcgggt tcggaaatag ccgtccatag ctataggtgg cgagtgtcag caaggccagc
     3601 agtgcgagtg cgcccgccac cttcggcctc caggcacgaa ccgctgccag catcatcact
     3661 ccggcgacac ccgtaatcac atgggcataa agagcccagc ggtgctcccg cagcgaaccg
     3721 cgagcgagca ggtacacgcc ggcaaaaacg gcggcggcaa aaagcacctg gctcgaagtg
     3781 gggattagcg tccgcagcgc ccggttgcgc acaagacgcc atgcgagcac caccgagagg
     3841 acaagcccca gaaacacgtg cagcaccacg ttcgccatat aaaagatcga aggcgtagcg
     3901 aaggcggcaa cgtacgctgt gttgattgca aacaacacca cggcggcggt ccatagccgt
     3961 ttcatctagg ttgccctcca gatcccagtg ggcgtagaag ctgtcgtgct ggcctaacat
     4021 tgtactcccg ccggtaggcg ctgtgaacgg gcgtatcggg ataccgttcc gccggcggat
     4081 aggggtaggc gctcatgcgg cgaaacggca gcggctccac agcctgcgaa aacgcggtat
     4141 tcgcgtcgcg atctttggcc cacccttcga ccaggagcag aaaatcacgc gaaaagcccg
     4201 aaggcagcgc gggcagacgc cgtgcatcaa aacgcaatcg cagttcatcc ccggagccca
     4261 tgatcacgaa gcggtcgtcg ccttctttga gcagcgggcc aacggcgcca tagcgcgtgt
     4321 acaggcccgg cgtctggttc cacatcgaaa ccggcagagg atcggggtat agaaaatgct
     4381 ccggctgcct acgctgcgga tgaatcacga ctcgcgaaaa accacggaag ctcaggtccg
     4441 catcaggcgg gagagctgtc accttggtct gaggcggagc cgtgtcttca ctcaggaaaa
     4501 tttcatccca gtagacggcc aggtttgtca cgatgcgaat ctcgcgcgag gaagaaagga
     4561 atttgccggt caggtccacg acaattgtct tcggcttgcc cgccggcatc cccatgtctt
     4621 cgaccactgt ctgccacttc ccctgcgcat cccgcacttc gagatacgga gtaatcaggc
     4681 cgccgcggcc ctcctgcgcc tcagccagaa acgtactgcc gtccgcccag tccacccagc
     4741 cgttcaacac cagcagcgcc cgattgtcac gcgccgcacc cgcgccgaaa tcgagttcca
     4801 gtgcccattt ttcggctact ccgttcatct tccggcggaa acgctcagcg tagatccgat
     4861 cgcggcggag cacgctggag gtaacatcaa aaccgttttg ccgcgcccga agcggcacca
     4921 tccggttccg gataccgaag agccggaact cgggaaacgg cgggccttta aacttgtcgt
     4981 tcgtaaagat ctcgatgtcc gcggcgtggt ctatcgcgat cagagagact ttatccaggt
     5041 atgcgacttc gctcaactct tccgtgatac ggatctcgta cgcgccgtcg cgctccgcaa
     5101 tcgcatcccg agggatctga atatactcgt cgtgatccac cgggaaataa ctgccatcgc
     5161 cggcgctcgc gccaagcggc gcgaccccga gtacatccgt gatgaatgtg aactcactcc
     5221 cgttccatga ccagatcatg gggcacgaac ccgaaagccg tggcttttct ttatacaggt
     5281 gcatcctgcc cgcgggttgt ctgatctcgt tttgaataag gccgttcggc cacatgatcc
     5341 gcaccgtgtc ggcctctttg tacggcccca ggtgaaacaa cagaggcact cccgtgtaga
     5401 tctgcttccc atacacgcgg cccgccttca cttccacctc ggcataatgc gccagcttca
     5461 ggttcttgac tccttccagc cgcaccgcta tccacgagcc gccgccggcc tggttgtccc
     5521 tgcgttgtat ggcgccgccg ggagtcacag agaccacatc gaaatcgcca tctctgtcga
     5581 aatccgccgt aaccgaaggc gcaccggagg cggcgattac ctcgaagcgc ccatcgcgat
     5641 tccggtccac cgcgtcgagc cccctggctc cttccgcaag tgtgtcgaga ggctctgctt
     5701 tgtaaacgcc ccccagttga tcccggtaga gcaccccttt acgctcgcgg tagctcaccg
     5761 ccaggtccat gctcttgctg tccggcacca gccggaacac aacgccgtcg agcgtctctc
     5821 cctgaacgaa cggaaagtcc gcggtgcggt cggcgaatcc tgccacaccc tggttgcgca
     5881 gcacacgcga ttctttcccc agcagcacga gatcgagatc gtaatcgtgg tcgtaatcga
     5941 gccagacagc tttgttgaaa acgccctcgg gcagcctggc ttcctgcagg ataaatttgc
     6001 ccttctggtt gcggtacagc acaactccgg agggagtcag caccgcgaca tcactcagtc
     6061 cgtcgttgtt gaaatcgccg ggcgccgccg agatcacagg accggcgggg aacgcgttcg
     6121 caaccagcgc cgacccgttc cggtacaagg ccagcccctg cccggaccac accagcagat
     6181 cggtgccgcc gtcaccatcg gcatcgatca gggccatccc ggcggtaccg gcatccacct
     6241 tgcctgcaag attgcgcgtg acgtattttg cgtccggctg cgcggctgcc gcaccgtcaa
     6301 tggtttcgta aacctcggcg taggcgttcc attccatgtc ctccggaatg gctgcgcctt
     6361 cctgttcttt tttgattttt tggaaaatct ccagttcgcg tttggcatcg gccggtcttc
     6421 cggcggctcg ataggcgttg aagagttgga aatgcggacc ggcaaggttg ggctcgagtt
     6481 tcgccgcgat ctcgaaatgt tttattgcct cggcatcccg gcccgggatc cggtaagagg
     6541 ctgccagatt gtaatgcgaa atcgcatcat ccggcaccag ctttaccatc tgctcgaact
     6601 gcgcgatggc ttgttccgct tcgccgcgcc tcttgtactc gatcccgaga ttgaaccagg
     6661 tgtgaggaag ggtggggtcc tgctgctgca cacgctccag ttccgccacg ccctcagcgg
     6721 ttttcccggc gcgcagcagc gccaggccgt agttca
//
LOCUS       DASTMK010000549        13631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_187834, whole genome shotgun sequence.
ACCESSION   DASTMK010000549 DASTMK010000000
VERSION     DASTMK010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13631
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187834"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFQ79_22635"
     CDS             complement(<1..305)
                     /locus_tag="VFQ79_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142537.1"
                     /translation="MDPKITLFVLICTAFAVSNSPAAQFGRGDVTFTPRFESTDSNRS
                     SGECKIRVRIDEEAEVIVNGNRVSIRTLRGRPGTDAGSECTSPLPVRGVQDFNFRKT"
     gene            complement(321..1244)
                     /locus_tag="VFQ79_22640"
     CDS             complement(321..1244)
                     /locus_tag="VFQ79_22640"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142538.1"
                     /translation="MIQRLLPFVTLALLFIGLSIASPHFLTNTNLSSVIRQTAVINIM
                     ALGMTLIIVAGGIDLSVGAILAFGGLTGTMAMAAGQPIIAGVAIGIVAGMFWGCVNGL
                     LTTRLRINPFIVTLGTLGIIRGITLIISNGLPVHNVPQAFSYLGEGLLLGVPFVLWIL
                     LACALAVHVTLEHTRLGRYAFAIGSNPDAAYYSGIPVAFHTTAVYAIGGMLTGLAGMI
                     EASRLMTGQPTAGQGYELQAIAAVVIGGGSLRGGEGSVIGTLIGAFIMGLLSNGSDLL
                     GISPYVQQAIIGAVIILAVSVDELRKRKMTV"
     gene            complement(1241..2749)
                     /locus_tag="VFQ79_22645"
     CDS             complement(1241..2749)
                     /locus_tag="VFQ79_22645"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU0142539.1"
                     /translation="MLLELKNIQKRFGGVVALRDGSLRVEPGQVHLLLGENGAGKSTL
                     MKIVAGIMDPDSGEIIWRGQRVQFRKPAGAAANGIAMVHQESLLAPHLSVAENVFLGR
                     ELCNRFGGVRRRRMQQETARLIEEHRFPLRAEWRVEKLSPAGKQMVEICRAICHGSSL
                     LIFDEPTSSLSEAETHEVFRIVRRLRERNMGVIYITHRLEELRAVGDRVTILRDGETV
                     HSGALQELPTERIIQLMVGREVTAIYRREPLPPGDVLLQVENISRAPLLHDISLKLRA
                     GEIVGLAGLVGAGRTEFCHTVFGLDPMDSGRILVAGREIQIRSPRDAVRAGIALIPED
                     RQRTGLATLLPTGYNLTLANLKSVSRFGFLNSVEERRVARTYIDRLRIKADSPKQLAG
                     RLSGGNQQKVAIAKWVARGARILLFDEPTRGIDVGAKVEVFQVMDELARAGAAILMVS
                     SELPELLQVADRILVMRQGRITGELPGRTTQEEIMSYAALEHSSGRTEENSR"
     gene            complement(2812..4179)
                     /gene="dnaB"
                     /locus_tag="VFQ79_22650"
     CDS             complement(2812..4179)
                     /gene="dnaB"
                     /locus_tag="VFQ79_22650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU0142540.1"
                     /translation="MSSADVALEKGLPVNLDAERYVLGSILIDESAYVQVAGILEPED
                     FSLEKHRRIFLRMNELHERGERIDRVTLANELIRHNQLESVDGISYLVSLDDGLPAIA
                     NLESYVRIVKEKSLLRRIIFASQKLINRCLIGDEQAGSILAEAEEGFLRLGESRDGNA
                     LARPGQIIEEFQGGLTAFLDPSKRIKGTGTGFRKFDEMTGGLHEGELIIIAARPSMGK
                     TALAMNIAQHVAGGESPRTAAVFSLEMSKESLLTRMICAGARVDQQKFRMGFLGQDER
                     RRLQMSATDLVEAPLFIDDTASVNLLDMHAKLRRVKAEHGLGLVVVDYLQLMTGRGRF
                     ENRNVEVSSISRGLKLLAKELRVPFIVLSQLSRATETRVGDHRPQLSDLRESGSIEQD
                     ADLVGFIFREEVYKPDREDLRGLAELIIAKQRNGPTGKVKLVFLREYTKFENPVSDLD
                     DEPAG"
     gene            complement(4417..6414)
                     /locus_tag="VFQ79_22655"
     CDS             complement(4417..6414)
                     /locus_tag="VFQ79_22655"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU0142541.1"
                     /translation="MLLYVLTISISAFLLFVVQPVIAKAILPWFGGSASVWATCMLFF
                     QFTLLAGYLYAHWSVRYLNARRQFLVHAGLLAGSLLLIPFTPNPGWKPAGYEDPSRLI
                     LALLITSIGLPYFLLSATSPLLQAWYARTRQSAAPYRLFALSNFASMLALLSYPVLIE
                     PFVSARIQARTWAAFYVFFAIVCAVVAYRARLTGLVPAVESELEATEPPRWYTWVLWL
                     GLASCASALLLSVTNHLTQDVASVPFLWILPLALYLASFVLCFESEGWYRPAIFHGLV
                     PLAVAGMCYVMYRQPSPEFEYLVPLMASGFLLLCMFCHGEIARTKPPPRYLTGFYLTI
                     ASGGAIGGLFVAVVAPRVFKGFFELPTSLVFAAVLFLIVFRKRARLYYYTWVALGLLL
                     VVFVVGESRAVAEGTKVFVRNFYGVLRVSDEGEGDYAVRKLLHGSINHGAQYLAEERR
                     MRPTTYYGPNSGAGIAIRNTRNSTQRVGVIGLGTGTLASYGRPGDYYRIYEINPLVVQ
                     VAKEEFTFLQDSKAHVDLVLGDARLSLEREGSQQFDTLLVDAFSGDSIPVHLLTREAF
                     EIYWQHLKPEGILAIHISNQYLDLQPVVYNLAAASGKEALLVDTEDDEDHEIFGSTWV
                     LVLSPQFNGGPEVRMASVPLTSGNNIHLWTDDYSNLFQILK"
     gene            6482..8677
                     /locus_tag="VFQ79_22660"
     CDS             6482..8677
                     /locus_tag="VFQ79_22660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142542.1"
                     /translation="MLRTVFPCFLFLIVPAILGAQSGGVLKGKVVLDPSGEAVHSATV
                     MIVQLGRSVQTDTEGAYQFNDVPPGTYDVLAHLHPLGDQRQRVTITSGAVAAVNFKLR
                     LAAVHEQITVTASGREETALEAFQTVTSMQTLELTAKSAPSLGEVLENEPGVAKRSSG
                     PGTTRPVIRGFDGDRVLVLQDGMPTGTLGYQSGDHGEPVDVTSLERVEVVRGPATLLY
                     GSNAIGGVVNAVTSHHQVHQHPHPGLRGHLTGMGGSNNGLAGGSGGFEYGYRNWLLWG
                     GGGRQRTGDYHAPLGKIENSQTDNSQASVGLGHYGSKYFASLGYGFQEGTYGIPAGVE
                     DLLHSEEGHDEHELVDLKFQRHNVRTGMGVKDFWSMERFALTLDYTDWHHREMSLGEV
                     HNRFYNKQFSYRGVFDQKKRGHNSGNFGFMGRYRDYKAIGEEAFAPPVTQTAFAAFAL
                     EQFSFEKFRIQLGGRIENTGYTPSGLRDRSFTGVSGAAGIQVPLWRGGSFVTNYTHSY
                     RAPALEELYSYGPHAGNLTFEIGDANLRREAAEGLDVSLRHEHSRIKGEVNFFQYWIG
                     DYVYLAPTGGIQDGLIEALYRQGDAHYQGIEGRIDAGICPNLWLNLGVDAVRATLTST
                     DMPLPRIPPLRGRIGMDVRYKGLSIRPGLLLTNQQDRIFPTETATAGYGVIDLAASYV
                     YTQKHAMHVFGVDTFNLGNNLYRNHLSFIKEIAPEIGRGIRAYYTIQFF"
     gene            8649..9593
                     /locus_tag="VFQ79_22665"
     CDS             8649..9593
                     /locus_tag="VFQ79_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142543.1"
                     /translation="MPTIRSSFFRNARVLAIAVLSVSAIFGQPLSRDAVRSHVNVIGI
                     SGEGKREVFSSDRLFEAPNWSPDGRYLVVNSEGKLWRLAIEGGEPELIPTGDVNRINN
                     DHGISPDGKWLAISAGPIYVLPWAGGVPRQVTQLAPSYYHGWSPDGKTLVYCARRDGN
                     FDIYAIDVSGGEEVRLTVHAGYDDGPDYSPDGKWIYFNSDRSGSWDIWRMPAAGAGPD
                     DKAAERITSDEMEDWFPHPSPDGKWLVFLSYEKGTKGHPANQKVVLRRMRLSPDASPA
                     PEIAEVVKLFGGQGTINVNSWSPDSKRFAYVSYSPRPH"
     gene            9727..9948
                     /locus_tag="VFQ79_22670"
     CDS             9727..9948
                     /locus_tag="VFQ79_22670"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU0142544.1"
                     /translation="MKKDELAQKLARQTHLSKAKAADELDRVVHDMIRELKKGHTVAL
                     PGIGTLKVGNRDSKRELPAKGPFGKGPRK"
     gene            9945..10526
                     /locus_tag="VFQ79_22675"
     CDS             9945..10526
                     /locus_tag="VFQ79_22675"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU0142545.1"
                     /translation="MNQKTLSTEDVALAVRRCLTAGRTVEIDGIGTFRPTADGGIEFA
                     PRQANRVFLAYVEEDAAEVERLYQRFEVAGLAPWMDRKKLLPGQNWPRSLEMAIGISD
                     FFVACFSKRAVSKRGHFQAELRYALQCASKLPLDEIFLIPIRLDECTVPARITREIQY
                     VDMFPNWEKGFQRVLATISQSGKSSKPQLRLAG"
     gene            10526..11332
                     /locus_tag="VFQ79_22680"
     CDS             10526..11332
                     /locus_tag="VFQ79_22680"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU0142546.1"
                     /translation="MPSARKGYHRSIIRTFALLLIASTAGAATLVLDVRTGRVLFEDT
                     AGNASAAPGSALKPFVAIALLETSTFRPAQRVTCTRQLQINGRKLDCSHPALAMPVDL
                     SAALAYSCNHYFATMSRRLDAARLAATLRRFGLNTGVAGTADQLALQAVGQWELECTP
                     AEFASAYRRLALFRRTAGARYEPLWRGIEGAVEFGTAQLAKPVSLRVAGKTGTTLNRN
                     RTGTLGWFAGWAPAGAPEFVVVVLTRGSGGPDAAPEARRAFERFAARGTP"
     gene            11332..12960
                     /locus_tag="VFQ79_22685"
     CDS             11332..12960
                     /locus_tag="VFQ79_22685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU0142547.1"
                     /translation="MPLAIVVLLLVAGACAQAAPGDYRVRLFWLHPPSELTLIPRGSV
                     RCNGSPLTASLLLRASADSVQSGQPRCSGPVVKIEGTVALKFPNGAAEFPHALEIRSR
                     RGRLLVTGTISLEQYVASVLLGESSVFTSAEALKAMAVAVRTYAVHYRGRHRTEGFDL
                     CDTTHCQDLRIAEAPPSRLISAVEATEGELLWYGGAPAATYYHRHCGGSTEDVESVWP
                     DRHAPYLKRQADTFCLSRGRAPWRSEVLASNLGPGPAPHILSRTASGRVAELDWNGRR
                     ITATRFHQRIGELFGWTTLRSNYYDVTQRGEHVIFQGFSAGHGVGLCQTGAEERGKAG
                     STYRQILAFYYPGTKLGINAQGFAWVQGRGELADVAAESGPTASELALLADRALKEAQ
                     RKTGLPLAFRPILRSYPSVAGFRDATGQPGWVAASTRGTVVRMQPITLLRSLGTLEAV
                     LLHESLHLLIESNAHPSLPEWFREGLALCLSDSDVALQQRGRAATKAEASHRATHRQA
                     KERVEQLLQRFGRQRVLGWVRSGLPGEALHPAGE"
     gene            complement(12874..13506)
                     /locus_tag="VFQ79_22690"
     CDS             complement(12874..13506)
                     /locus_tag="VFQ79_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142548.1"
                     /translation="MPGFMRRKWVLLAVQIAGYALVAILIYLWLGIPEESVWQLLLSV
                     LLAVGIIAGAGWLLASLFALRLCGRWEWMRGMAFVLLLLAMFLGGSWLSGSAPRVNPL
                     WFWSIGAFIVFAVLPWFAARAWLIRRWQYWASAVAVVALGIYIPWKLIWWVPSLSGMT
                     AQTASMAVRFGLAYLLLVISLLLFSGWMQRLSGKPAANPPEHPLAPETLE"
BASE COUNT         2976 a         4079 c         3980 g         2596 t
ORIGIN      
        1 tccgtcttcc ggaaattgaa atcctgaacg ccgcgaaccg gcagcgggct tgtgcattcc
       61 gaaccggcgt cggtgcccgg ccgtcccctc agcgtacgga tcgacacccg gtttccgttc
      121 acaatgactt ccgcttcttc atcaatccgt acgcgaatct tgcattctcc cgaggaacga
      181 ttcgaatccg tcgattcaaa ccggggagtg aaggtcacat ctcccctgcc gaactgtgcc
      241 gcgggagaat tggagacagc gaacgctgtg caaataagca caaaaagtgt gatctttgga
      301 tccataaaag taacccaatg ctataccgtc atctttcgct ttctgagctc gtccacactc
      361 acagccagaa taatcaccgc gccgataatc gcctgctgaa cataaggact gatgcccaac
      421 aggtcgctgc cattcgacag caatcccata atgaatgcgc cgataagcgt gccaatgacg
      481 ctgccctcgc cgccgcggag gcttccgccg ccgattacga cggcggcgat ggcctgcaac
      541 tcatagccct gaccggcggt aggctggccg gtcatcaacc gcgatgcctc aatcataccc
      601 gcgagaccag taagcattcc accgatcgca tacacggccg tagtgtgaaa cgcgacggga
      661 atgccgctgt agtaggccgc gtccggatta cttccgatag cgaacgcgta acgccccagc
      721 cgcgtgtgct ccagcgtaac gtgcaccgcg agcgcgcagg cgagcaaaat ccagagcaca
      781 aagggcactc ccaacagcag accttctccc aggtaggaaa acgcttgcgg gacgttatga
      841 accggcaggc cattggaaat gatgagggtg attccgcgaa taatcccgag cgtgcccagg
      901 gtcacgatga agggattgat gcgaagccgc gtcgtcaaca agccattcac acatccccag
      961 aacataccgg cgacaatgcc gatcgccact ccggcaatga tgggttggcc ggccgccatc
     1021 gccatggtac cggtcaagcc gccgaaggcc agaatggctc cgacggatag atcgatgccg
     1081 ccggcgacga taatcaacgt cattccgagc gccatgatgt tgatgacggc ggtctggcga
     1141 atcacactcg agaggttcgt attcgtcagg aaatgcggtg acgcgatcga aagcccgatg
     1201 aagagcaatg ccagcgttac aaacggcagg agccgttgaa tcatcgcgag ttttcctccg
     1261 tcctgccgga gctgtgttcg agcgccgcat agctcatgat ctcttcctgt gtagttctac
     1321 ccggcagttc tccggtaatt cgcccctggc gcatcacgag gatccggtcg gctacctgga
     1381 gcagttcggg gagctcggag cttaccatca gaatggcggc cccggcgcgg gcgagttcgt
     1441 ccatcacctg aaagacctcg accttggctc cgacatcgat gccccgcgtg ggttcatcga
     1501 acaggagaat gcgcgcgcct cgtgctaccc atttcgcgat ggcaaccttc tgctgattcc
     1561 cgccgctcaa gcggcccgcc agttgtttcg gagaatccgc cttgatgcgc aaccggtcga
     1621 tgtaggtccg ggcaacacgt ctctcctcca cactgttcaa aaacccgaac cggctgaccg
     1681 atttcaggtt tgccagcgtc aggttgtatc cggtcggcag cagggttgcc agtcccgttc
     1741 tctggcggtc ttccggaatc agcgcgatgc cggcgcgcac agcatcacga ggcgagcgta
     1801 tctgtatttc gcgtcccgcg accaggatgc ggccggagtc catcgggtcc agaccgaaca
     1861 ccgtgtggca gaactccgtg cggcccgcgc cgaccaagcc ggccagtccc acgatttcac
     1921 cggcccgcag cttcaggctg atgtcgtgca gcagcggcgc acggctgata ttctccacct
     1981 ggagaagaac gtcgccgggc ggcaggggtt cgcgccggta aatggccgtt acttcacggc
     2041 ccaccatgag ttgaataatg cgctcggtgg gaagctcctg gagtgctccg gagtgtacgg
     2101 tttcgccgtc ccgcaatatg gtgacgcggt ctcctaccgc gcgcagctct tccaggcggt
     2161 gcgtgatata aatgacgccc atattgcgct cgcggagcct gcgaacgatt cgaaatactt
     2221 cgtgtgtctc ggcttcggag agcgacgagg tgggctcgtc gaagatcagc aggctggagc
     2281 cgtgacagat ggcccggcag atttccacca tctgctttcc ggccggactc agcttctcga
     2341 cgcgccattc cgcgcggagg ggaaaccggt gctcctcgat caatcgcgcg gtttcctgct
     2401 gcatgcggcg gcggcggacc ccaccaaacc ggttgcacag ctcacggccc aaaaagacgt
     2461 tttcagcgac agagagatgt ggcgccagca gagattcctg gtgaaccatg gcgattccgt
     2521 tggccgccgc tccggccggt ttacgaaact gcactctctg gccgcgccag atgatttcgc
     2581 cactgtcggg gtccatgatg cccgcgacaa ttttcatcag ggttgacttg cctgcaccgt
     2641 tctcgcccag cagaagatga acttggccgg gctccacgcg aaggctccca tcacgcagcg
     2701 cgaccactcc gccaaaacgt ttttggatgt tcttgagctc gagaagcacg aaggaaacaa
     2761 caagtttaca tgaagtgcca atgggcgcgg atcgccgggg ctgcgctact ctcagccggc
     2821 cggctcgtcg tccaggtccg acacggggtt ctcgaatttc gtgtactcgc gaaggaacac
     2881 cagcttcact tttccggtcg gcccgttacg ctgtttggcg ataatcaatt ccgccaggcc
     2941 ccgcaagtct tcgcggtcgg gcttgtatac ctcttcgcgg aaaatgaacc ccaccaggtc
     3001 ggcatcctgc tcgatgctgc cgctttcgcg cagatcgctc aattgcgggc ggtgatcgcc
     3061 cacccgcgtt tccgtggcgc ggctgagctg ggacagaacg atgaacggaa cgcgcagctc
     3121 cttcgccaga agtttcaacc ctctcgaaat ggagcttact tcgacgttcc tgttttcaaa
     3181 ccggccgcgg cccgtcatga gttgcaggta gtcgaccacc accagcccga ggccgtgctc
     3241 cgctttcacg cgccggagtt tcgcgtgcat gtccagcaga ttgacggatg ccgtgtcgtc
     3301 aatgaacagc ggcgcttcca ccaggtctgt ggcggacatc tgcaatctcc ggcgctcatc
     3361 ctggccgagg aagcccatac ggaatttctg ctgatccacc cgggcgccgg cgcagatcat
     3421 cctggtgagc agcgattcct tcgacatttc gagcgagaac accgcagccg ttcgtggcga
     3481 ctcaccgccg gcgacgtgct gtgcaatgtt catggccaaa gccgttttgc ccatggatgg
     3541 ccgggcagcg atgatgatca gttcgccttc atgcagcccc ccggtcatct cgtcgaactt
     3601 ccggaaaccc gtaccggtgc ccttgattcg cttgctgggg tccagaaaag cagtcaggcc
     3661 gccctggaac tcttcgatga tctggccggg ccgcgccagc gcattgccgt cgcgcgattc
     3721 accaagccgg aggaatcctt cctccgcttc ggcaaggatg cttccggctt gttcgtcccc
     3781 gatgagacac cggttgatga gcttctgcga tgcgaatatg atccgccgga gaagcgattt
     3841 ctcctttacg atgcgaacat agctttccag gttggcgatt gccggcaggc cgtcgtccaa
     3901 cgatacgaga taactgatcc cgtcgacgct ttccagttga ttgtgccgga tcagttcgtt
     3961 cgcaagcgtc acacggtcta tgcgttcgcc gcgctcgtgc agttcgttca tccggagaaa
     4021 gatccggcgg tgtttctcga gactgaaatc ctcaggctcg agaatacccg cgacctgcac
     4081 gtaagccgat tcgtcgatca gaatggaccc gagcacgtat cgctcggcat ccaggtttac
     4141 cggaagccct ttctccagcg cgacatcagc ggatgacatg attcggaagt cctaccataa
     4201 tcgttttgcc ggcttacggc aaaccgaagt tagcaccctc tggtacgaac cgcttagcct
     4261 gtgaaaaaat ctgtgaaaaa cctcgatgtt tttccacagc cgccccgcgt cagaagcgcc
     4321 tttctggaag gcctcttacg ggcagagcac cagagttttc cacagtttag cgcaaaacag
     4381 aacttaaccc ggacgtttta ccgttttcac gaaggatcac ttcagaattt gaaacaggtt
     4441 gctatagtcg tccgtccaga gatggatgtt attgcccgag gttaacggaa ccgaagccat
     4501 ccgtacttcc ggcccgccgt tgaattgtgg cgacaacacc agaacccacg tagaaccgaa
     4561 aatttcgtga tcctcgtcgt cctcggtgtc caccaggagc gcctccttcc ctgaagcggc
     4621 ggccaggttg tagaccaccg gctgcaagtc gagatactga ttgctgatat gaatggccag
     4681 aatcccttca ggcttcaagt gctgccaata aatctcgaat gcttccctgg tcagaagatg
     4741 gacaggaata gagtccccgg aaaaagcatc gaccaaaagg gtatcgaact gttgagagcc
     4801 ttcacgctcc agcgacaacc gggcatctcc cagcacaaga tcgacgtgag ctttgctgtc
     4861 ctggaggaag gtaaattcct ccttcgccac ctggacgaca aggggattga tctcatagat
     4921 acggtaataa tcgcccggcc gcccgtagct cgccagagtg cctgttccaa ggccgatgac
     4981 acccaccctt tgcgtggaat tacgcgtgtt gcgaatggcg attcccgctc ccgagttcgg
     5041 accgtaatac gtggtgggcc gcatacggcg ttcctcagcg aggtactgcg cgccgtggtt
     5101 gatagatccg tgcagcagtt tgcggacagc gtaatcgccc tcaccctcgt cggaaacacg
     5161 taaaaccccg tagaagtttc gtacgaacac cttcgtcccc tcggccacgg cacgggactc
     5221 gccgacgaca aacactacta gcaacagacc aagcgccacc caagtgtagt agtacaacct
     5281 ggcccgtttc cggaaaacga tcagaaacag caccgcggca aacaccaggc tggtgggtaa
     5341 ttcgaagaac cctttaaaca cacggggcgc gaccaccgcg acgaaaaggc cgccgattgc
     5401 ccctcccgaa gcgatcgtca aataaaaacc ggtgaggtac ctgggcggcg gcttggttct
     5461 cgcgatctca ccgtgacaga acatgcatag aagtaaaaaa cccgacgcca tcagcggtac
     5521 cagatattcg aattcgggcg acggctgccg gtacatcaca tagcacatcc ctgccacggc
     5581 gagaggtacc aggccgtgaa aaatcgccgg gcgataccag ccttcgctct cgaaacagag
     5641 aacgaaactc gccaggtaca acgccagggg cagaatccat agaaacggca cagaggcaac
     5701 gtcctgcgtc aggtgatttg tcacggagag caacaacgca gatgcacaac tcgccagccc
     5761 cagccacagg acccatgtat accagcgcgg tggctcagtt gcttccaatt cggattcgac
     5821 cgccggcaca agccccgtca agcgggcgcg gtatgccacc accgcgcaga cgatggcgaa
     5881 gaaaacatag aaggccgccc acgtcctggc ttgaattcgg gctgaaacga atggctcgat
     5941 caacaccgga tagctgagca gcgccagcat ggaagcaaaa ttagacagcg caaacaggcg
     6001 atatggcgca gccgactggc gtgtcctggc ataccatgcc tgcagcagag gactggtggc
     6061 tgagagcagg aaatagggca gccctatcga cgtgatcagc agagccagaa tcagtctgct
     6121 tgggtcttcg taaccggcgg gcttccatcc cggattgggc gtgaatggaa tcaataagag
     6181 gctgccggcc aaaagccctg cgtgcacaag aaactgccgg cgcgcattca aataacgtac
     6241 agaccagtga gcataaagat aacctgccag caacgtgaac tgaaaaaaca gcatgcaggt
     6301 ggcccataca gacgccgagc caccaaacca gggcagaatc gcctttgcga tgacaggctg
     6361 gacaacaaag agcaaaaacg cgcttataga aattgtcaga acgtacaaca gcatcgatta
     6421 agggaactca actcaacgat aacatcgcgg cgccgagcct atgccaaact gaaataagta
     6481 gatgctcaga accgttttcc cctgtttctt atttctgatt gtaccggcga tactgggagc
     6541 ccagtccggt ggcgtgctga aaggcaaagt agtactggat ccaagcgggg aagcagtgca
     6601 cagcgcgaca gtcatgatcg ttcaacttgg caggtcggtt cagaccgata ccgagggagc
     6661 gtatcagttc aatgacgtgc ctccgggaac ttatgacgta ctcgcgcatc ttcatcccct
     6721 cggagatcag aggcagcgcg taacgataac ttccggcgcg gtggctgcgg tgaatttcaa
     6781 gctccggctt gccgcggtac acgaacagat cacagttacg gcttcgggaa gagaagagac
     6841 ggcactcgag gcgtttcaaa cagtcacctc catgcagacg ctcgaattga ccgccaagag
     6901 cgctccttcg ctgggcgaag tgctggaaaa cgaacccggt gtggcgaaac gcagctcggg
     6961 tccggggacg acaagaccgg tcatccgcgg cttcgacgga gaccgtgtcc tggttcttca
     7021 ggacggcatg ccgaccggca cgctcggtta tcaatcgggc gaccatggcg aaccggtcga
     7081 cgtgacgtca ctcgagcggg ttgaggttgt gcgcggcccg gcaacgctgc tgtatggcag
     7141 caacgcgatc gggggagttg tgaatgcggt tacatcccat caccaggtgc accagcatcc
     7201 tcaccccgga ttgcgcgggc atttaacggg catgggcggt tccaacaatg gactcgccgg
     7261 gggtagcggt ggattcgagt atggctatcg gaactggctg ctctggggcg gcggcgggcg
     7321 gcagcgcacg ggtgactatc acgcgccgct ggggaagatt gaaaactcac aaacggataa
     7381 ttctcaagcc agcgtaggcc tcggccacta cggcagtaag tatttcgcaa gcctcgggta
     7441 tggctttcaa gaaggcacat acggcatacc ggcgggtgta gaagacctgc tacatagtga
     7501 agaaggccac gacgaacatg aacttgtgga tctgaagttc cagcgccata acgtcagaac
     7561 gggaatgggc gtgaaggact tctggtcaat ggagcgcttc gcactgacac tggactacac
     7621 cgactggcat catcgcgaga tgtctttggg cgaagtacac aatcgcttct acaacaaaca
     7681 gttctcctac cgcggtgtgt tcgatcaaaa gaaacgcggc cataattccg gtaatttcgg
     7741 cttcatgggc cgctaccgcg actataaagc gattggagaa gaagcgttcg ctccaccggt
     7801 aacgcagacc gcctttgccg cgtttgcgct ggagcagttc agctttgaaa aattccgcat
     7861 ccaactcggc gggcgtatcg aaaacactgg ctacacccct tcaggacttc gggatcgttc
     7921 cttcacaggc gtctccggag ccgccggcat tcaggtaccg ctgtggcggg gcggcagttt
     7981 cgttacgaat tacacgcact cctatcgcgc accggcgctt gaagagctgt attcctatgg
     8041 gccgcatgcc ggcaatctga cgttcgagat cggcgacgcc aatttgcggc gggaagccgc
     8101 ggaagggctc gatgtgtccc tgcggcacga gcattcccga atcaaaggcg aggtaaactt
     8161 cttccagtac tggatcgggg attacgttta tctcgcaccg acgggtggga tccaggacgg
     8221 gttgatcgag gccctgtacc ggcagggcga cgcgcattac cagggaatag aaggccgcat
     8281 cgatgcagga atctgtccca acctgtggct gaacctcgga gtggatgccg tccgcgcgac
     8341 tctcacgagc acggatatgc cccttcctcg aatccctccc ctgcgagggc gtatcgggat
     8401 ggatgtccgc tacaagggct tgagcatccg ccccggacta ctgcttacga atcagcagga
     8461 tcgaatcttc cctaccgaaa cggcgacagc gggatatggc gtgatcgacc ttgcggcatc
     8521 gtatgtgtac acgcaaaagc acgctatgca tgtgttcgga gtggatacct tcaatttagg
     8581 gaataacctg taccgcaatc acctgtcttt catcaaagag attgcgcccg agataggacg
     8641 aggtattcgt gcctactata cgatccagtt tttttaggaa tgcccgcgtc ctggcgatcg
     8701 cagtactatc ggtatccgct atattcggac agccgctcag ccgcgacgca gtgcgcagcc
     8761 atgtaaacgt tatcggcatc tcgggtgaag ggaaacgcga ggtattcagc tcagaccggc
     8821 tctttgaagc accgaactgg tcacccgatg gaagatacct ggtagtgaac tccgagggca
     8881 aactctggcg gctggcaatc gaagggggcg agccggaact gattcccacc ggcgatgtga
     8941 accgcattaa taacgatcac ggtatatcgc ccgatgggaa atggctcgct atctccgccg
     9001 gaccgattta tgtactgcca tgggccggcg gcgtgccaag acaagtaaca cagcttgctc
     9061 ccagttacta tcatggttgg tctccggacg gcaagacact cgtttactgt gcacggcgtg
     9121 acggtaactt cgatatttat gccatcgatg taagtggcgg cgaggaggtc cgtctcaccg
     9181 tgcacgcggg ctatgacgat ggtcccgact attcgccgga cgggaagtgg atctatttca
     9241 attcggaccg ttccggaagc tgggatatct ggcggatgcc ggccgctgga gcggggccgg
     9301 acgataaggc cgcggaacgc atcaccagcg acgagatgga agactggttc ccccatccgt
     9361 cgcccgacgg aaagtggctc gtctttctct cttatgaaaa gggtacgaaa gggcatcccg
     9421 ccaatcagaa ggtcgtcctt cgcaggatgc gcctttcccc ggacgcctct cccgctccgg
     9481 agatcgccga ggtggtgaaa cttttcgggg gccagggcac gatcaacgtg aattcgtggt
     9541 cgccggacag caagcgcttc gcctatgtaa gttactcgcc ccggccgcac tgagagaggc
     9601 gcggtcacaa accggcgcct gtacctgcca gcccaggcaa ttgccgtatt cacaatacgt
     9661 tagcctgtat cccggacgcc ccgcacaccc tctggtttca aggaccgttg ctacgatgta
     9721 actggcatga agaaagacga gttagcgcaa aaactcgcca ggcagacaca cctgagtaaa
     9781 gccaaggccg ccgacgaact cgaccgggtg gtccatgaca tgattcggga gttgaaaaag
     9841 gggcacaccg tggcgttgcc gggaatcggg acgctgaagg ttggcaaccg cgactcgaag
     9901 cgcgaacttc cagcgaaggg acctttcggc aaaggcccac ggaaatgaac cagaagaccc
     9961 tgtcaacgga agatgtcgct ctcgctgtgc ggcgttgcct cacggccggc aggacggttg
    10021 agatcgatgg gatcggcacg ttccgtccca cagccgatgg cggcatcgag ttcgcaccga
    10081 ggcaggccaa tcgtgttttt ctggcttacg tagaggaaga cgccgcggaa gtcgagcggc
    10141 tgtaccaacg tttcgaagta gccgggctgg ccccgtggat ggaccggaag aaactgctgc
    10201 cggggcagaa ctggccgcga tcgctcgaga tggccatcgg catctccgat tttttcgtcg
    10261 cgtgtttctc caagagggcc gtgtccaaac gcgggcactt tcaggcggag ctccggtacg
    10321 cacttcaatg cgcgtcgaaa ctgcctctgg atgaaatctt tctgatcccg atcagactgg
    10381 acgagtgcac cgtgccggct cgcattactc gcgaaattca gtacgtggat atgttcccca
    10441 actgggaaaa aggctttcag cgcgtgcttg ccacgatatc ccaaagcgga aaatcgtcga
    10501 agccgcagct cagactcgcc ggatagtgcc gtccgcccgc aagggatacc atcgaagtat
    10561 catccgcacg tttgcactac tactgatcgc ctccaccgcg ggcgccgcca cgctggtctt
    10621 agacgttcga accgggcgcg tgctttttga agacaccgca ggtaacgcaa gcgccgcacc
    10681 gggttccgcg ttgaaaccgt tcgttgccat tgcgcttctt gaaacctcaa cctttcgccc
    10741 ggcgcagcgc gtgacctgca cacgccagct acaaatcaac gggcgcaagt tagattgttc
    10801 gcaccctgct ctcgccatgc ctgtggacct ttccgcggcg ctcgcctatt cctgcaacca
    10861 ttacttcgcg acgatgtcac gccgcctcga cgcggcgcgg ctcgccgcca cgctgcggcg
    10921 ctttggcttg aacactggag tcgcgggaac tgccgatcaa ctcgccttgc aggcagttgg
    10981 gcagtgggag ctggaatgca cacccgcgga gttcgcctcc gcctaccggc ggctggccct
    11041 ctttcggcga acggctggag cccgctacga acccttgtgg cgcggtatcg aaggtgcagt
    11101 ggagttcggc acggcgcagc ttgcaaaacc ggtaagcctg cgcgttgccg gaaagaccgg
    11161 cacaacactg aatcggaatc gaacaggaac cctgggctgg tttgccggat gggctcccgc
    11221 cggggcgccg gaattcgttg tcgtcgtgct tacgcgaggc agcggcgggc cggatgcggc
    11281 tccggaagcg cgccgggcat ttgagcggtt tgctgcgaga ggaactcctt gatgccgctg
    11341 gcgattgtgg tgctcctgct cgtagcgggc gcgtgcgcac aagcggctcc cggcgattat
    11401 cgcgttcgcc tcttctggct tcatcctccc tccgaactga cgttgattcc gcgcggcagc
    11461 gtgcgttgca atggctcgcc tctcaccgca tcgttattgc tccgggcgtc ggcggattcc
    11521 gtacaaagcg gccagccccg ctgcagcggg ccagtggtaa agatcgaagg gacggttgcg
    11581 ctgaagttcc cgaatggcgc cgccgaattt ccccacgcgt tggagatccg atcccgacgc
    11641 ggccggctcc tggtaacagg cacaatctcc ctggaacaat atgtggcgtc cgtgctgctc
    11701 ggtgaaagca gcgttttcac gtcagccgaa gctttgaaag cgatggcggt ggcggtgcga
    11761 acctacgcgg tacattaccg ggggcgtcac cgcacagaag gattcgacct ctgtgacact
    11821 acacactgtc aggatttgag gatcgcggaa gcgccgccgt cgcggttgat ttcggccgtg
    11881 gaagcgaccg aaggagagtt gctgtggtat ggcggcgcac cggctgcgac gtactatcac
    11941 aggcattgcg ggggctcgac agaggacgta gaaagcgtct ggcccgatag gcacgctccg
    12001 tatttgaagc ggcaagcaga caccttctgt ctttcgcggg gacgtgcacc gtggcggtcg
    12061 gaagtgctcg cgagcaacct tggacccgga ccggcgccgc acattctaag ccggacggca
    12121 tccggacgtg tggccgagct tgactggaat ggccggcgca tcacagctac ccgatttcac
    12181 caacgaatcg gtgagttgtt cggttggacc acgctgcgca gcaattacta cgacgtgact
    12241 caaagaggcg aacatgtcat ctttcaggga ttcagcgccg ggcatggagt gggcctgtgc
    12301 cagacgggcg ccgaagaacg gggcaaagcg ggcagcactt accggcagat tctggcgttc
    12361 tactatccgg gcactaagct gggaatcaac gcgcagggat tcgcgtgggt gcaagggcgc
    12421 ggcgagttgg cggacgttgc ggcggaatcc gggcccaccg ccagtgagtt ggcccttctg
    12481 gctgacaggg cgctcaagga agcgcagcgt aagacaggat tgccgctcgc gttccgcccc
    12541 atcctacgca gctatccaag cgtagccgga tttcgcgatg ccacaggcca gccgggatgg
    12601 gtagccgcga gcacgcgcgg aacggtggtg cgcatgcagc ccatcacgct gcttcgatcc
    12661 ctcggaacgc tggaagcggt gctgctgcac gaaagtctgc acctgttaat cgaatcgaat
    12721 gcacacccgt cgctgcccga atggttccgc gagggacttg cgctttgcct ctcggattcc
    12781 gatgtggcgc tacaacagcg gggccgcgcc gccacaaagg ccgaggcttc gcatcgcgcc
    12841 actcaccggc aggcaaagga acgggtcgag cagttactcc aacgtttcgg gcgccaaagg
    12901 gtgctcgggt gggttcgcag cgggcttccc ggagaggcgt tgcatccagc cggagaataa
    12961 gagcagcgag ataaccagca gcaggtacgc gagaccgaag cgcacggcca tgctagccgt
    13021 ctgagcagtc atgccggaaa gcgacgggac ccaccatatc agtttccagg ggatgtaaat
    13081 acccagggcc acaacagcca ctgccgacgc ccagtactgc catcgccgga tgagccatgc
    13141 gcgtgcggcg aaccagggga gcaccgcaaa cacgatgaac gctccaatgc tccagaacca
    13201 caacggattc acgcggggcg ctgaaccgct cagccagctt ccacccaaga acatcgccag
    13261 caggagcagt acaaacgcca tcccgcgcat ccattcccag cggccgcaca gccggagagc
    13321 gaacagcgac gccagcagcc agcccgcgcc ggcgatgatc ccaaccgcga gcagcactga
    13381 gagtagcaat tgccagacgc tctcctctgg aattccgagc cagaggtaaa tgagtatggc
    13441 taccagcgca tatccggcga tctgcactgc gagcaaaacc cacttccggc gcatgaagcc
    13501 cggcatcact tcacctccaa agtgcggctt ggagtagccg cggaatgctg tggctcatac
    13561 atcggctgca cacgtgccgg acttactcga aattgtccgg gacttacaac ttttagtaag
    13621 taaacgtgct c
//
LOCUS       DASTMK010000550         4806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_124387, whole genome shotgun sequence.
ACCESSION   DASTMK010000550 DASTMK010000000
VERSION     DASTMK010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124387"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="purD"
                     /locus_tag="VFQ79_22695"
     CDS             <1..781
                     /gene="purD"
                     /locus_tag="VFQ79_22695"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU0142549.1"
                     /translation="TAEAMLAGELVGDAGRRLLVEEFLKGEEVSFIAITDGRTVIPLE
                     ATQDHKAIFDGDRGPNTGGMGAYCDGRILPPSATEDVLSTVVLPVVEYMRDKEEPFTG
                     FLYAGLMLTGDGPKVLEFNVRLGDPETQALMMRLNSDFAGLLMECARGALSDTRPGWS
                     TDPSVCVVMAAAGYPGQVRTGDSITGIAEAERDGAVVFQAGTARANGEVRTAGGRVLG
                     VTASGADLPAAIARTYDAVGRIRFEGAHYRTDIGVKGLKRW"
     gene            795..871
                     /locus_tag="VFQ79_22700"
     tRNA            795..871
                     /locus_tag="VFQ79_22700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:829..831,aa:Arg,seq:cct)
     gene            894..1520
                     /locus_tag="VFQ79_22705"
     CDS             894..1520
                     /locus_tag="VFQ79_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0142550.1"
                     /translation="MPQSPKLCPGNEEPESVTRLLVEMQHGSHAAEEALLPLIYDELR
                     RMAANYMRREKPDHLLQATALVHEAYLHLVDQSETTWKNRAHFFGVAAQMMRRILVDH
                     ARSEKAWKRGAGAKRISLDQVGPLFHKREVALDELDDALRVLEEIDPRQGRIVELRFF
                     GGLTTEETAEVIGVSATTIEREWRSAKAWLYDQLKRTEPPSTTKAENP"
     gene            1684..2868
                     /locus_tag="VFQ79_22710"
     CDS             1684..2868
                     /locus_tag="VFQ79_22710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0142551.1"
                     /translation="MTPERYQRIKQLFEAVVGQPDGSRGSFLDRECGDDPQLRSQIES
                     MLESDQEGADFLEAPAVVSFSRLLDREPESLVGQRIGPYKILGELGKGGMAVVYQATR
                     DDLQSDSTVAIKFIKSSVGSDIIARRFRQERQILAALEHPNIAQLLDGGTLEDGRPFF
                     VMEYVEGKPIDVYAREQQLNLRQRLELFLSVCSAVEYAHQNLVIHRDLKPANILVRGD
                     STVKLLDFGIAKLLNPSIGGGAADKTASGIRLMTPEYASPEQLRGEPVTTVSDIYLLG
                     IVLYELLTGYRPYRTSGNSAVEAMRALAERAPEKPSVVAAGDSRRPGEPPKQKSLTPD
                     DLAGDVDAITLKALERDPAWRYSSARELAQDIRRHLDGNPVLARSRSLPYLFRKLVKR
                     PT"
     gene            complement(2870..4222)
                     /gene="mnmE"
                     /locus_tag="VFQ79_22715"
     CDS             complement(2870..4222)
                     /gene="mnmE"
                     /locus_tag="VFQ79_22715"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU0142552.1"
                     /translation="MDLSDTIVAISTPPGRGGLGVIRISGPAARRVAEQFLEFSPQFS
                     WRPWKATLAGLPDSNRRIIDRVIVTYFEKPHSYTAEDVIELSCHGSPVVLRHAIECAC
                     LAGARLAEPGEFTLRAYVNGRIDLPQAEAIRDLIDATTLFQARIAAQQMDGSVSRRIA
                     PLKEQLVELVSILEAGIDFAEDDISVASPEEITRRIDSILSGLESLAGSFAYGKLVHS
                     GLTLAIVGRPNVGKSSLFNRLLDQDRAIVTDIPGTTRDLVSETASISGIPVRFVDTAG
                     IREGKDPVERLGIERSYQAMADADLTIVVLDASAKAESEDLELIEHARRSGRFLLVAN
                     KTDLGVTLELPGEAIPVSALTGDGIDRLKESINESIAPPGVLESEAGFITSLRHEQLL
                     RESMEAIKEARTATEAGIPHEMLLLDLYAGLRPLDAITGATTADDILNRIFSTFCIGK
                     "
     gene            complement(4226..4783)
                     /locus_tag="VFQ79_22720"
     CDS             complement(4226..4783)
                     /locus_tag="VFQ79_22720"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEU0142553.1"
                     /translation="MGQKKYRVETVGPRIEAFLNSTRERIGLRVKFEVADGETPHPDF
                     ENPEIMVRFSGPDVELLLANKAELLLALEQLTMEMLRMPAEEHSLLCFDANDYRVLRV
                     EELRLSALAAADKVKRTRVPFEFSPMNSRERRIIHLALRNEPTLRSESVGMAPHRHVV
                     IYPSDMPSKPDTASVPAFARRGRRR"
BASE COUNT          997 a         1409 c         1433 g          967 t
ORIGIN      
        1 gacggctgaa gccatgctgg ctggcgaact ggtaggcgac gcgggccgcc gtctcctggt
       61 agaggaattt ctgaaaggcg aggaggtcag cttcattgcg atcacagacg gacgcaccgt
      121 gataccgctt gaggcgaccc aggatcataa agctattttc gatggcgacc gtggacccaa
      181 cacaggcggc atgggtgcct attgcgatgg ccggattttg ccgccgtctg cgacggagga
      241 tgtcctgtca accgtcgttc ttccggttgt cgagtatatg agggacaaag aggagccttt
      301 caccgggttt ctttacgcgg gattgatgct gacgggggac ggtccaaagg ttctggagtt
      361 caatgtgcgc ctgggcgatc cggaaacgca ggctctaatg atgcggctga acagcgattt
      421 cgcgggcctt ctcatggagt gtgcccgagg cgcactaagc gatacccgtc ccggctggag
      481 tacagacccg tccgtgtgcg ttgtcatggc cgccgccggg tatcctggtc aggtccggac
      541 gggcgactct attactggaa tcgcggaggc cgaacgggac ggtgccgtgg tgtttcaagc
      601 gggaacggca cgcgcaaacg gcgaagtgcg tacggccggt ggacgggtgc tgggcgtcac
      661 cgcttcgggc gccgatcttc cggcagcgat cgcgcggacc tacgatgccg taggccggat
      721 ccgctttgag ggtgcccatt atcgcaccga tatcggtgtc aaaggtctga agcgctggta
      781 gaatgtgtag acgcggggac gtagctcagt tggatagagc ggtcgcctcc taagcgacag
      841 gtcggcagtt cgaatctgcc cgtccctacc atatcagtgt gtcgaggagg gcagtgccgc
      901 aatctccgaa gttgtgccct ggcaacgagg aacccgagag cgtaacccgg ctcctcgtcg
      961 aaatgcagca cggcagccat gccgctgaag aagcgttgct gcctctcatt tatgatgagc
     1021 tccggcgtat ggccgcaaac tacatgcgcc gcgaaaagcc ggatcattta ctgcaagcaa
     1081 ccgcgcttgt gcacgaagct tatctccacc tggtagacca aagtgaaacc acatggaaga
     1141 atcgggctca ctttttcggt gtagccgccc agatgatgcg ccgcattctg gtggatcacg
     1201 cgcgcagtga aaaagcatgg aagcgcggtg caggcgccaa gcggatctcc ctcgaccagg
     1261 tcgggccgtt gtttcataag cgcgaagtgg cgctcgacga gctcgatgac gctctccggg
     1321 tactcgagga gattgatccc cgccagggcc ggatcgtcga gttgcggttc ttcggcgggc
     1381 tcactactga agagacggca gaggtgatcg gcgtgtcagc cactactatt gagcgtgagt
     1441 ggcgtagcgc taaagcctgg ctctacgacc agttgaaaag aacagaacca ccctcaacca
     1501 ccaaagctga aaatccgtga cggttttgaa cccgatcctg cgcgttagcc attgaagacg
     1561 gatccaccgt ctcgaccttc gccccgtgat cgcaaagggg caccatggca cccgcagctc
     1621 ggaggaacgg ccggataacg tatccggccg tctgcggatg ggcgatacta gaagtgcgcc
     1681 cgcatgacgc ccgagcgtta ccaacggatt aaacaactct ttgaggcggt ggtcggccaa
     1741 ccggacggca gccgtggctc ttttctcgac cgcgagtgcg gcgacgaccc tcaactccgt
     1801 agccagatcg agagcatgct ggagtccgac caggagggcg cagactttct ggaagcgcct
     1861 gcggtcgtat cgttctcccg tttgctggac cgcgagccgg agtcccttgt aggccagcga
     1921 atcggcccct acaagatact gggtgagctc ggtaagggcg gcatggctgt tgtttaccag
     1981 gctacccgtg acgatcttca gtccgatagc accgttgcga tcaagttcat caaatcgtcg
     2041 gtcggttccg atatcattgc acgccggttc cgccaggagc ggcagatcct cgctgccctg
     2101 gaacatccca atattgcgca actgctcgac ggcggcacac tggaggacgg tcgtcccttc
     2161 ttcgtgatgg aatatgtcga aggcaaaccg atcgacgtct acgcccgcga acaacaactg
     2221 aatcttcgcc aacgtctcga actatttctt tcggtttgtt cggcggttga atacgcgcac
     2281 cagaacctgg taattcatcg tgatctgaaa ccggccaaca tcctcgtgcg aggcgacagc
     2341 actgtgaagt tgctcgactt cgggattgcg aagctcctga atccaagcat cggcggaggt
     2401 gccgctgata agacggcaag tggcataagg ctcatgaccc ccgagtacgc gagtccggaa
     2461 caacttcggg gcgagcccgt tacgaccgta agcgacatat atctgcttgg gattgtgttg
     2521 tacgagctgc tcaccgggta ccggccctat cgaacgtccg ggaattcggc ggtggaagcg
     2581 atgcgggcac ttgcggaaag agcgccggag aaacctagcg tcgttgctgc cggcgattcg
     2641 cggcggcccg gtgaaccacc gaaacaaaaa tccctcacgc cggacgatct ggccggagac
     2701 gtagatgcca tcacgctgaa ggcgcttgag cgcgatccgg cgtggcgcta tagttccgcc
     2761 agagagcttg cccaggacat ccgccggcat ctggacggca atcctgttct ggcgagaagc
     2821 cgctcgctac cgtacctctt tcggaaactc gtgaagcgcc cgacgtaggc tacttaccta
     2881 tacagaacgt ggaaaaaatc cggttcagaa tatcgtccgc cgtggttgcg ccggtgattg
     2941 cgtccagtgg ccggagcccc gcatacaggt cgagcaggag catctcgtga gggataccag
     3001 cttctgtggc agtacgcgct tctttgatag cttccatgct ttcacgtagc aactgttcat
     3061 gccgtaggct cgtaatgaaa cccgcttcgg attcgaggac tccgggaggc gcgatgcttt
     3121 cgtttataga ctccttaagc cggtcgattc catcgccggt cagagcagat accggaatcg
     3181 cctcaccagg gagttcgagc gtgacgccaa ggtccgtttt gttcgccacc agcaggaaac
     3241 ggccactccg gcgtgcgtgt tctatcaatt ccagatcttc ggactccgct ttcgccgatg
     3301 cgtccaggac aacaatcgtc agatcggcat cagccatggc ctggtagctg cgttcaatcc
     3361 cgagccgttc caccgggtcc ttcccctcgc gaatgccagc cgtatccaca aaacgaaccg
     3421 gaatcccgga aatcgaagcg gtctcggaca ccaggtcccg cgttgtgccc ggaatgtccg
     3481 tgacaattgc tcgatcctga tccagcaacc ggttgaacaa gctgctcttg ccgacattgg
     3541 gccgtcctac gatggcgagt gtgagccccg agtgaaccag tttcccataa gcaaaactgc
     3601 cggcaaggga ttcgaggccg ctcaaaatcg aatcgatcct tcgcgtgatc tcctcaggcg
     3661 aagcgacgct gatgtcgtcc tcggcaaagt cgattccagc ctcgaggatg gacacgagct
     3721 cgaccaattg ctccttgaga ggcgcgatgc gccttgaaac cgatccatcc atctgctgtg
     3781 cggcgatgcg cgcctgaaaa agggtggtcg catcaatcag gtcccgaatc gcctccgctt
     3841 gcggcaggtc gattcttcca ttcacataag cccgcaacgt gaattcaccg ggttcggcca
     3901 gccttgcacc ggcgaggcag gcgcattcta tcgcatggcg caatacgacc ggagatccat
     3961 gacacgaaag ctcgatgacg tcttcggcgg tgtaggagtg tggcttttcg aaataagtca
     4021 ctatcacccg gtcgatgatt cggcggttgg agtccgggag accggcgagc gttgccttcc
     4081 agggccgcca tgaaaactgc gggctgaatt cgaggaactg ttccgccacc cttcgggctg
     4141 ccggaccgga tatgcggatc acccctaatc cgccccttcc gggaggcgtg gaaatggcga
     4201 caatggtgtc gctaagatcc aaaacttaac ggcggcgccc gcgccgggcg aaggccggga
     4261 cgctggccgt gtccggcttg gaaggcatat cggaagggta aataacgacg tgccggtgcg
     4321 gcgccatgcc cacactctcg cttcggagcg tcggctcgtt acgcaaagcc agatgaatga
     4381 tccgccgttc ccggctgttc atcgggctga attcgaacgg cacgcgtgtg cgcttcactt
     4441 tgtccgccgc cgccagggcg ctcaaccgca gctcttccac gcgcaaaact ctataatcgt
     4501 tcgcatcgaa acagagaagc gagtgctctt ccgcgggcat gcgaagcatc tccatcgtca
     4561 attgctccag tgccagcagc agttcagctt tattcgcgag cagaagttcc acatccggtc
     4621 cggaaaaccg caccatgatc tcggggtttt cgaagtcagg atggggtgtc tcgccgtcag
     4681 cgacttcgaa tttcacccgc aagccaatcc gctcgcgggt ggaattcaga aacgcttcaa
     4741 ttcgagggcc gaccgtctca acccggtatt tcttttgacc caccggctac tttttcgacc
     4801 tcctct
//
LOCUS       DASTMK010000551         5018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_347791, whole genome shotgun sequence.
ACCESSION   DASTMK010000551 DASTMK010000000
VERSION     DASTMK010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347791"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFQ79_22725"
     CDS             <1..984
                     /locus_tag="VFQ79_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142554.1"
                     /translation="TMKLLVSLAGKENLMAIPQRLQEVVMTPRDANRFRTPGPLGHSN
                     TASGTRVAGPLGFQPGIFAAPPKLQIKAVKKLKMPAPAGDSKTKLVRDVSWDLFKKGK
                     DPAVADVHQGQLANCPLASLLSALANTPSGRKHIKSLIAEYSAVVETDVSAVAGELDS
                     VPAGKKITSNRYFAVTLGGQSIEVSSVFYTDDADEGWSLIYMRSPNEALWPCLIEKAY
                     AHKEGNYGALDSGSGKQLTLNQIWKVVVGSAPEGFEVNKKTPVSKISSTISAAGRIPT
                     VAASDAMWHGFTVLGMKGSKIELYDPMAPGKKTVSMEDFRNDFSAILYGSP"
     gene            complement(999..1460)
                     /locus_tag="VFQ79_22730"
     CDS             complement(999..1460)
                     /locus_tag="VFQ79_22730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0142555.1"
                     /translation="MERMISGMLENYERGNLGRRQLIQGLAALMMTAVPAPAESTFKG
                     VGLNHIALRVTDIRRSREFYQKHLGFPLISESETSCFLRAGEEFLALFRNERPGLDHY
                     CIAIENFKPDAVMAELNRQGLKPRRPSGTDRIYFPDMDGLEVQLSAVDHRA"
     gene            complement(1535..2437)
                     /locus_tag="VFQ79_22735"
     CDS             complement(1535..2437)
                     /locus_tag="VFQ79_22735"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0142556.1"
                     /translation="MRFLFVFLVCAVAHLNAADRHVAITIDDVPRGGDGGSRSLTDIR
                     AMTERLLRPFRDQKIPVIGFVNEGRQTGFGPEGLRQILDLWLDFGADLGNHSYSHLNI
                     NQVPLSRYTADIVKGEPILRAALAGRGKTLRFYRHPFLFTGPAPEIKKKMQEFLDDHG
                     YQVAPVTLDNSDYQYAALYTRPPYRERVRREYVPYMESIVAFFEKRSVEVVGREFPQI
                     LLLHANELNADLLPDLLAMFRRRGYTLISLEQALADEAYRSPDEYAGRGGFSWIHRWS
                     ITKGMKPMGEPEPPPWVQEAWAAR"
     gene            complement(2463..4019)
                     /locus_tag="VFQ79_22740"
     CDS             complement(2463..4019)
                     /locus_tag="VFQ79_22740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU0142557.1"
                     /translation="MSLLNVSGLSFRYPSTIELFRDVTFAIETEDRLAIVGANGVGKS
                     TLLRILAGELEPAGGTIARRKDLQTATVDQYGAAGEGSLFDYVFGARPHLARLRMRLA
                     ATEREAPCEYAELVNEYEAFGGYAAEARTGSILSGLGFARGEWSLPPSSLSGGQRTRA
                     GLARALHTEAGLLLLDEPTNHLDIAAREWLEDQLAGRGACVLVSHDRTLLRKVANRVL
                     EIERGRVTLFEGGYDAYRARRALAERQSWENYEAFERRKAAAEQAAERRSRLATQVAT
                     APRGERSSHDFYRRKAAKVARTSRLLRERVHREPEVIKPWEEQPIPVLEFAGVRRSGD
                     IALSVTNLSKSYPGKPLFAGITFELARGSRMAITGPNGSGKTTLFRVLLGLEPAGTGT
                     VRFGAHVVTGYYAQDAGNLDGSLAPLQLCGSGTLARTLLACLKVRPDRIDHPLAEASA
                     GERAKTALVRLLISGANLLVLDEPANYLDIEAQEALEKTLAQYPGTILLASHDRSFLE
                     AIAPEEVLQL"
     gene            4201..>5018
                     /locus_tag="VFQ79_22745"
     CDS             4201..>5018
                     /locus_tag="VFQ79_22745"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142558.1"
                     /translation="MNSALRRIVIGVIAAICFIGMNGTAYAQILYGSIVGNVKDSTDA
                     PVPGASVSITSRGTSQSRQTTTNEAGGYSFPTLLPGSYDVTVVKQGFQTVTRSGVEVT
                     INSVSRTDMMLEVGIVAESITVSEGAATLQTDRAEVRAELQSRTLQNIPLPSGRNYQQ
                     MFVAIPGFSPPADASSIPGNPSRALQVNVNGTTSNSAVTRIDGASSTNVFMNMVSAYV
                     PALESIETVNVVTNSFDAEQGLAGGAAINVQIKSGTNQLHGSAFEYHTNNKLKA"
BASE COUNT         1045 a         1507 c         1446 g         1020 t
ORIGIN      
        1 accatgaaac tattggtttc gctagctggg aaagaaaacc tgatggcgat tccacagagg
       61 cttcaggagg tggtgatgac gcctcgagat gcgaaccggt tccgtactcc gggccctctc
      121 ggacactcga ataccgcgtc ggggaccaga gtggcgggtc cactgggatt tcagccaggc
      181 attttcgccg caccgcccaa gctacagatc aaggctgtaa aaaaactgaa aatgccggca
      241 ccggccgggg actcgaagac gaagcttgtg cgggatgtgt cctgggatct tttcaagaag
      301 gggaaggatc cggcagtggc cgacgtgcac cagggacaac tggccaattg tccattggca
      361 tctcttctga gcgcccttgc taataccccc agcggccgca agcacatcaa aagtctgatt
      421 gcggaataca gtgctgttgt agagaccgat gtctccgccg tcgccggcga gctcgacagc
      481 gtgccggcgg gcaagaaaat cacctccaac cgctatttcg ctgtgaccct gggtgggcag
      541 tcgatcgagg tgagcagcgt attctacacg gacgatgcgg atgaaggttg gagtctcatc
      601 tacatgaggt cgcccaacga ggcgctctgg ccctgcttga ttgagaaggc atacgcccat
      661 aaagagggta actacggagc gctggattcc ggatctggta aacaactgac cttgaatcag
      721 atctggaagg tggtggtggg ctcggcgccg gaaggattcg aagtgaacaa gaaaacgccg
      781 gtttcgaaaa tctcctccac gatctcggcg gctggcagaa ttcctactgt tgcggcttcg
      841 gatgccatgt ggcacggttt caccgtactt ggaatgaagg gttccaagat cgagctgtac
      901 gatccaatgg cgcccggcaa aaagacagtc tcgatggaag acttccgcaa tgatttttcc
      961 gcgattctgt acggcagccc gtgatctgtt ccggtctctc aggcgcgatg gtccaccgcg
     1021 gagagttgta cttcgagccc gtccatgtcg gggaagtaaa tgcgatcggt cccactgggg
     1081 cgtctcggct tcaacccctg acggtttagc tcagccatca ccgcgtccgg cttgaaattt
     1141 tcgattgcaa tgcagtagtg atccagcccc ggacgttcgt tccggaatag cgccagaaac
     1201 tcttcgccgg ctcgaaggaa gcagcttgtc tccgattcgg agattaacgg aaatcccaag
     1261 tgcttctgat agaactcgcg agaacggcga atatcggtta cacgaagagc gatgtggtta
     1321 agtcccactc ccttaaacgt ggattcggca ggcgccggca cggcggtcat catcagcgcc
     1381 gctagtcctt gaattaattg tcggcggccc agattacctc gctcgtaatt ttcgagcatc
     1441 cccgagatca tacgctccat aagagtttcc ctccaggttt gcactgctgg ctggatcata
     1501 atcccccgcg gtccgccttt tcaacaccgc ctcgctatcg tgcagcccaa gcttcttgta
     1561 cccatggcgg cggctccggc tctcccattg gtttcattcc cttggtgatt gaccagcgat
     1621 gaatccagga gaagccgccg cggcccgcat actcgtcggg actacggtag gcctcgtcgg
     1681 ctagcgcctg ctccagtgag atgagcgtgt aaccgcgccg gcggaacatg gcgaggagat
     1741 caggaagcag gtccgcattg agctcgttgg catgtaggag gagaatctgg ggaaactcgc
     1801 gacccacaac ttcgacggaa cgtttttcga agaacgcgac aatggattcc atatagggca
     1861 cgtattccct ccggacgcgc tcgcggtatg gcggcctggt gtaaagcgcc gcgtactggt
     1921 agtccgaatt gtcgagcgtg accggcgcta cctggtatcc gtggtcgtcc aggaactcct
     1981 gcatcttttt tttgatttcc ggtgccgggc cagtgaacag gaacgggtgg cgatagaaac
     2041 gcagcgtctt tccgcggccg gccagggccg cacgaagaat gggctcaccc ttcacgatgt
     2101 ccgcggtgta ccggctcagc gggacctggt tgatgttgag atgcgaatac gaatggttcc
     2161 ccaagtcggc accaaagtcg agccagagat cgaggatttg tctgagtcct tcagggccga
     2221 agccggtctg acgtccctcg ttgacgaagc cgatcaccgg gattttctga tcgcggaaag
     2281 gacgcaggag gcgttcggtc atggcgcgga tgtccgtcag gctgcgggag ccaccgtcac
     2341 caccgcgcgg tacgtcgtca atcgtgatcg ccacatgccg gtccgcggcg ttcagatgcg
     2401 ccaccgcgca caccagaaac acgaacagaa atctcatgtt caacggtata ccgtcatcat
     2461 tgctatagct gcaacacttc ttccggcgcg atcgcttcca gaaagctgcg gtcgtgtgat
     2521 gccagtaaga tcgtccccgg atactgggcc aatgtcttct ccagtgcctc ctgggcttcg
     2581 atgtcgaggt agttggccgg ctcgtccagc accagcaggt ttgctccgct gatcagcagg
     2641 cgcaccagcg ctgtcttggc gcgctcgccg gcgctcgcct ccgccagtgg atgatcgatg
     2701 cggtccggac gcaccttcag acaggccagc agcgttcgcg ccagtgtgcc tgaaccgcag
     2761 agctgcaaag gagccaatga gccatccaga ttcccggcat cctgcgcgta atacccggtc
     2821 acgacgtgcg ctccgaatcg cacggttccg gtgccggcgg gctcgaggcc gaggagcacc
     2881 cgaaacagcg tcgtcttgcc tgacccgttc ggcccggtga tcgccattcg actaccgcgt
     2941 gccagttcaa aggtaatacc cgcgaacaac ggcttccccg ggtaggattt cgacagattc
     3001 gtaaccgaca gggcgatgtc accgctgcgt cgcacacccg caaactcgag gaccggaatc
     3061 ggctgctctt cccaaggctt gatgacttcg ggttcgcgat ggacacgttc ccgtagcagc
     3121 cgcgacgtcc gcgccacttt ggccgctttc cgcctgtaaa aatcgtggct gctgcgctcg
     3181 cctcgcggcg ctgttgccac ctgcgtggca agacgcgaac gccgttcggc agcttgctca
     3241 gcggcagcct tccggcgttc gaaggcttcg tagttctccc aggactgccg ctccgccagc
     3301 gcccggcgcg cgcggtatgc atcgtagccg ccttcaaaga gtgtcacccg cccgcgctcg
     3361 atttccaata cccgattcgc taccttccgc agcagcgtac ggtcgtggga gaccagcacg
     3421 caggcaccgc gcccggcaag ctggtcttca agccattcgc gcgcggcgat gtcgaggtga
     3481 ttggttggct cgtccaacag cagcaggccg gcctcggtgt gcaatgcgcg agccaggccc
     3541 gctcgcgtgc gctgcccgcc tgacaagctc gatggcggca ggctccactc gcctcgcgcg
     3601 aatcccaacc cgctcaggat gctcccggtt cgcgcctccg ctgcgtagcc gccaaaggcc
     3661 tcatactcgt tcaccagctc ggcgtactca cacggagcct cgcgttccgt cgcggccaaa
     3721 cgcattcgca accgcgccag gtgaggccgg gcgccgaaca cataatcgaa cagggagccc
     3781 tcgcccgcag ccccatactg atccacggtt gctgtttgca gatctttccg ccgcgcgatc
     3841 gtgccgccgg ccggctccaa ctctccggcc aggatgcgca gcagggttga tttgcccacg
     3901 ccgttcgcgc cgacgatagc caggcggtct tccgtttcga ttgcaaacgt cacgtcgcgg
     3961 aacagttcaa tagtggatgg ataccggaac gaaagcccgg agacattcag taaagacata
     4021 cactcctcgc agccgccgtg cgcggccatt ggcatgaact ggagttgcct ttaccgtctc
     4081 atagatgcag acgaaagtct gcgaaccaat tatacgaaat agacggttcg gaatgccagt
     4141 ttatgatatg atccgcttgt tttcaccaaa cattttgggg tgcgttgcag gaggattatc
     4201 gtgaattccg ctttacgtcg aattgtaatt ggcgttatcg ctgccatttg tttcatcggg
     4261 atgaatggca ccgcgtacgc tcaaatcctt tatggttcga tcgtcggcaa cgtgaaagac
     4321 tcgaccgacg cgcctgtccc gggcgcatcg gtatccatca caagccgcgg aaccagtcag
     4381 agccgccaga ccaccaccaa tgaagccggc ggctacagct ttccaactct actacctggg
     4441 agctacgacg ttacggtcgt caagcagggg ttccagactg ttacgcggag cggcgtggaa
     4501 gtgactatca attccgtttc gcgcaccgat atgatgctcg aagtcggcat tgtggcggag
     4561 tccatcaccg tcagcgaagg cgcggctact ttgcagaccg atcgcgccga agtgcgggct
     4621 gaattacaaa gccgtaccct ccagaacata ccattgccgt ccggacgcaa ttaccagcag
     4681 atgtttgttg cgattccggg gttcagcccg ccagcggacg catcttcgat tcccggaaac
     4741 ccgtcacgtg ctttgcaggt gaatgtgaac ggcacaacca gtaattcggc tgtaactcgt
     4801 atcgacggcg ccagcagcac aaatgttttc atgaacatgg tttcggccta tgtgcccgcg
     4861 ctcgaatcca ttgaaacagt caacgtggtg accaatagct tcgatgcgga acaagggttg
     4921 gccggtggtg cggccatcaa cgtccaaatc aaaagcggca ccaaccaact gcacgggagc
     4981 gcattcgaat atcacaccaa caacaaatta aaagccaa
//
LOCUS       DASTMK010000552        12898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_604442, whole genome shotgun sequence.
ACCESSION   DASTMK010000552 DASTMK010000000
VERSION     DASTMK010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604442"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VFQ79_22750"
     CDS             complement(<1..739)
                     /locus_tag="VFQ79_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142559.1"
                     /translation="MAIRRIRVLSRGVPVFAILLLCGPLFAGRTTGPQPQRDEAQQNQ
                     PRKFTLGEPAPAEPVLLPPPPVLQWSPFGTRELRPSLFDQREALLGRSRDLIPPPPGS
                     VTRAILGFVALLVLAYLGGHRSVQRFEQRFNIAHLATAGLPFLFLGLIAAQPGIGILS
                     PEVLKEIAPLLSLGLGWIGFAVGSRFNARALDDLPPGTSAAVFLTTAFPIFAIFVVCA
                     VVLAASGQSVAESGYLRDALLLGTAGAM"
     gene            788..2143
                     /gene="xseA"
                     /locus_tag="VFQ79_22755"
     CDS             788..2143
                     /gene="xseA"
                     /locus_tag="VFQ79_22755"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU0142560.1"
                     /translation="MSQLTISWEPERRIFSVSELSAAIRGLFEDQFSDIWVSGEISGT
                     KTASSGHCYFSLKDDGAQVRCVCFRGTVRYLKFKPQDGIAVLARGRLDVYESRGEYQL
                     LVEALEPQGYGALQLAFEQLKKKLAAEGLFDAARKRALPKYPARIGIVSSPAGAVIQD
                     ILQILSRRFPGIHIRLYPALVQGEGAAAAVSRGIGYFSRSGWPQVVIVARGGGSLEDL
                     WTFNEESVARAIAACSVPVISAIGHETDFTIADFVADVRAPTPSAAAEIVICTRQELL
                     DGISACARKMEQALRYRLAAAGRRLHEQGVDRATTVLHRRIGRFSQRVDELEYALRDR
                     LRSALLRRSTALSGLEQRLRRMDLRLRFAAAHRRLAFAHAEAIQAMRFRLTAARGRFD
                     PLNAQLAQLSPLRVLDRGYAIVLDERGEILKDAAQTSPGAQLEIRLSRGRVGARVTER
                     K"
     gene            2207..3118
                     /locus_tag="VFQ79_22760"
     CDS             2207..3118
                     /locus_tag="VFQ79_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU0142561.1"
                     /translation="MLRDLLLYLSTQKHLRNWVETSPLAGRLTSRFIAGNTLEEALAV
                     ATRLNAEGLLVTLDHLGENVTSEVEANASRDAYLQALGRIGQLGLRATVSIKLSQFGL
                     AISEQLCRENVAVVVEQASRMRTWVEVDMEASRYVDATLALVREMHSRHQAVRAVIQS
                     YLRRSEHDIEQLCRDRIPVRVCKGAYKEDDSIAFPEKEQVDANYLRLSEILLERGVYP
                     GMATHDPKMVQGVLDFASKRSISPEQFEFQMLYGIRRDLQRMLIRRGFRLRLYVPYGS
                     AWYPYFMRRLAERPANILFLARNLFRW"
     gene            3158..3598
                     /locus_tag="VFQ79_22765"
     CDS             3158..3598
                     /locus_tag="VFQ79_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142562.1"
                     /translation="MKYWGYLVAKSTVAAVLIYTLWRMLWVVMPEPGYMVFQQTRRFG
                     QDLWWTTAILVLWLLSAALIYLIIWDQRRRCRTCLRRLRMPVETGSWKNLLLIGPPRT
                     EYICPYGHGTLSVPELHITGMESPDWQPHEDIWAELYGTEETRK"
     gene            3595..3945
                     /locus_tag="VFQ79_22770"
     CDS             3595..3945
                     /locus_tag="VFQ79_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142563.1"
                     /translation="MRRFASWRIAAGAVVLACLIGFLGLLVPSYLRNREFQNFLDRTV
                     NSELAHRMPPGALQASVADGAGRIGLPVAPDQVRVRQTADDLRIEVRYAVRVEIPLYT
                     VDLHFRPAAVSRRQ"
     gene            3986..6499
                     /locus_tag="VFQ79_22775"
     CDS             3986..6499
                     /locus_tag="VFQ79_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU0142564.1"
                     /translation="MAVRFQIPRKAGLVRLLLHPAGKFVAVSLTVALIAGIGAFTYYY
                     AKYSRLIEQKLSAGPFPTTSMLFAAPKIVSVGDETVASDIVALLRRSGYSESRTNRMG
                     WYRLTSDGIEVYPGPDSYFRQEPGVIRFQSGTIARIISLADNTERTRYQLEPELITNL
                     FDRKREKRRIVGFSDIPRVLINAVISIEDKRFFQHPGFDPFRVIKSAYVDLKKGYLAE
                     GASTLTMQVARTFWLNPDKNWSRKLAETLIALQLEQKLTKEQIFEYYANQVDLGRRGS
                     FSIRGFGEAAQAYFGKDIQQLSLSEAATLAGLVQRPSYTNPYRWPERARARRNVVLSL
                     MRENGYISDVEYAVAAASPLVIKKGGVGSGDAAYFVDLVNDELQGDFEGHDFQEDSYR
                     VYTTLDVDLQEAAAEAVRIGMQDVDAQLQSRRKKNPNYPQAQVALVALDTQTGEIKAL
                     IGGRNYGMSQLNRALAKRQPGSVFKPFVYTAALSMGFDDASRAVTSATTLLDEPTTFW
                     YNGQAYEPSNYKHQFYGLVTVRQALAKSLNIPTVKLAEMAGYERIVEVARNAGMNLAI
                     QPTPAVALGAYEVTPIEIAGAYTIFANQGTYVKPSWIKYIRDSSGRAIYDYKPKRQQA
                     IDPRVSYVMEQMLEEVLRTGTGAGVRARGFSLPAAGKTGTSHDGWFAGFTSKLICAVW
                     VGFDDNRELNLEGARSALPIWTEFMKRAHQLREYRNARPFPAPEGVIQVEIDPTTGQL
                     ATPQCPTVRTDFFIAGTQPVEVCHYHGGTSQIAGWETPPPATPTPVTLPPAAPGNSNI
                     VRTSRGAPPQVTTKAETPTEEREQPKKRGFFGRIRDIFR"
     gene            6516..7304
                     /locus_tag="VFQ79_22780"
     CDS             6516..7304
                     /locus_tag="VFQ79_22780"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142565.1"
                     /translation="MVSLTFAVFAAGSASFLLAGQSDGLKQPPAIPPSAAASGEAQPR
                     SANTTSEMRGDALMDRKNYQEAIVTYQDAVRDQAILMNKIGIAYHQLTDLDTAKRYYE
                     RALQINPNYSEAINNLGTVYYAKKDYGKAEREYKKALKLTPESASIYSNLGTAYFARK
                     RYDDAMQAYQKALSLDPEVFEHRSSAGVLLQERSVAERAKFHFYMAKTYAKAGVFDRA
                     LLYMRRALEEGFTERDRFRQDPEFAQMQEMPEFQQLLLLEPRVL"
     gene            7438..9141
                     /locus_tag="VFQ79_22785"
     CDS             7438..9141
                     /locus_tag="VFQ79_22785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142566.1"
                     /translation="MLLAGCSSSPEKSGAQRTAILPFENLTGDRSLEWMGRAASEIVI
                     ESLSGSPSITALRVSSANEAYAVRAERLVEGYYALVDGKLRLTASVRDIERNRTIDSP
                     FAEGAVASGILPVAAGMARNLGGDTNRFGTRNNIAIENWAKGLAGTAGEQTSRLFEAA
                     IAADPDFGEAYVHFAEFLLGRPDVAAARRIIESARSRNLGEVERTRLDVLSASIAGDA
                     RAQLEAVQRLATLTPGDAVLYRTLGNLQLSAHRYGDASKALARAAELDPADPDAWNLL
                     GYARAYTLDLEGAKAALEQAAKTTPADANPQDSLGDVHFLLGRFREAESYYLAAHGKI
                     PSFLGSATLLKAAHARWMAGDLPGADTIFQQYTDALRQAKSPLAELQAARWEYLTGRL
                     QQGVERLEKFAATAQGDVRALAFSQLAVWKLAGGIRTGAAQSAAEAAKSASSPAARVT
                     AYLAAAVIRGGTAGGDQSANPTLRDYALAYSALLEKRFADAVPPLERLYGQSSFTSGR
                     RIELVLAWAYIETGALDRAKKLLQTYPLPVIQPEEIFAFLEYPRVLELRKKVGLKNHG
                     S"
     gene            complement(9186..12488)
                     /locus_tag="VFQ79_22790"
     CDS             complement(9186..12488)
                     /locus_tag="VFQ79_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142567.1"
                     /translation="MRKVSVFSTFLVVLMALSLAIPPSFCQEVSAGITGAVTDATGGA
                     VAQAAITARDVDRGTTWNTTTNEQGIYFFPRVPPGNYELRIEAKGFKAAVRPGIVLES
                     TQRARVDVALEIGAVTESIEVTGSAPLLQTDRTEVGGVITTNRLTNSPMISRNYIILT
                     LLNPGVTTTNPAAFNNDQRTTGGGRPYVNGNRKEANNFLLDGIDNNQVSDNLTSYQPN
                     LDAISEVRMITNNASAEFGNFQGGIINVLMKSGTNDLHGSLFHFWRNDKLNANNWARN
                     WNNLPRSYLRYNSFGGTVGGPLTIPKLFDGRDRLFFFADYMGMRKPTPATIQTITVAP
                     AGFRQGDFSRLLTEQNRQLYNPFDLDANGRRRPFPNNQIPMSMMSPVARALYGDQSLY
                     PLPLNSDLRLNQTNANSSQLNLDQGDIKVDAKLSSNDDLSVRYSQSMQNNPGFNTFPL
                     TFNSFNESPFKAGVLNWTRTFSPTVVNEARAGVNRITLHNGGANKGYPDLNEQFGIAG
                     VNSPGLLSIEFPNGFTSSIGNANVGTQQLFANTTYHFADNLTIIRGRHMIKTGGQLLR
                     QQMNTFYAGNNGRTGLIRFSGRFTGADSSGIGWADADFFLGLPETLGRGLNTGTWGHR
                     KTIYGLYAQDDWRVSDTLTLNLGLRWEYHTPLVEVNDRQANFEPFSGRLLLAGQDGNS
                     RALYEPFKKDFQPRVGFAWTFLPQTVMRGAYTISSFMEGTGTNLRLPFNPPFNAEFEG
                     RYDRSDLNLPLSNASQGLTALQQADPFSRVNIRLWDPFVRPANTQQWNLTLERQLPAQ
                     LVLTAGYVGQKGHHLVVPMPYFQRQLMPDGTTRPSPYLSGNPQLANIAQISGTESNGN
                     QQYHALQMMARKRMGSGLEFQTSYTFSKGMSDAIGYYGEGGQSANQSAYWQNLYDRKA
                     EWGPTYFDARHMLTFTHVYELPFGQGKRFGTDMHPVLMGILGNWQLGGILTLRSGFPL
                     TIQGPDNSNTNSRGARADRIGDGGKVGDVGRGTRWFDTSAYRIPAAGTFGSTGIGTER
                     GPGLKQYDVSIQKQFPIGERYRFEFRAEFFNLTNTPQFSAPARAVQGATFGEITGAQG
                     ERQGQLALKFYF"
BASE COUNT         2613 a         3768 c         3905 g         2612 t
ORIGIN      
        1 ccatcgctcc ggcagtgccg agcaggagcg cgtcacgcag atatccggac tccgcgacag
       61 actggcccga cgcggccagc accaccgcgc acactacaaa gatggcgaaa atcggaaatg
      121 ccgtcgtgag gaagacggcg gcgctggtgc ccggcggcaa gtcgtcgagt gccctggcat
      181 tgaaacgcga accaaccgca aagccgatcc aaccgagccc cagtgacagc agcggcgcga
      241 tctctttgag aacttccgga gatagaatgc caatgccggg ctgcgccgca atcaggccga
      301 gaaaaaggaa tgggagccct gctgtcgcca ggtgtgcgat gttgaatcgc tgctcgaaac
      361 gctggacgct gcggtgcccg ccaagatagg ccaacaccag cagcgcaacg aatcccagaa
      421 tcgcccgggt aacagagccc ggcggtggag ggatgagatc gcgcgagcgg ccgagcaatg
      481 cttctctctg gtcgaacaac gacgggcgca gttcccgggt gccgaaggga ctccattgca
      541 gaaccggcgg cggtggcagc aacaccggct ccgccggcgc gggttcgccc agggtgaact
      601 ttctcggctg gttctgctgt gcttcgtccc gctggggttg cggacctgta gtccgtccgg
      661 cgaaaagcgg cccgcagagt aacaggatgg cgaaaaccgg aacaccccgg gaaagcacgc
      721 ggattcttcg gattgccatc ttttccgagt atcccagaac atccccctgt taatctgaac
      781 agtaggcatg tcgcaactga ccatttcctg ggagcccgaa cggcgcatct tcagcgtgtc
      841 cgagctgagc gccgccatac gcgggctgtt cgaggaccag ttcagcgaca tatgggtctc
      901 cggagagatc tccggcacga aaaccgcctc gagcggccac tgctatttca gcctcaaaga
      961 cgatggcgca caggtgcgtt gcgtgtgctt ccgcggcacc gtgcgctatc tcaagttcaa
     1021 accgcaggac gggatcgccg tactggcgcg gggccggttg gatgtctatg agtctcgcgg
     1081 tgaatatcag ttgcttgtcg aagcgctgga gccccaggga tatggcgcgc tccaacttgc
     1141 cttcgagcag ttgaaaaaga aactcgccgc ggaaggcctt ttcgacgctg cacgcaagag
     1201 agcccttccg aaatatcccg cccggatcgg cattgtgtcc tctccggcgg gagccgtcat
     1261 tcaggacatt cttcagatcc tcagccgccg gtttcccggt attcacatcc ggctctatcc
     1321 cgctctcgtt caaggcgagg gggcggcagc ggcggtttcg cgcggtatag ggtatttcag
     1381 ccggagcggc tggccgcagg tggtgattgt cgcgcgcggc ggcggctcgc tcgaagatct
     1441 ttggactttc aacgaggaaa gtgtcgcgcg agcgattgcc gcctgctctg tgccggtcat
     1501 ttcggcgatc ggccacgaga cggacttcac cattgcggat ttcgtcgcgg acgtgcgggc
     1561 tcccacgcct tcggccgccg ccgagattgt gatctgcacc cggcaggaac tgctcgatgg
     1621 catctcggct tgtgcccgca aaatggagca ggctctccgc taccggcttg ccgccgccgg
     1681 ccgccgcctg cacgagcagg gagtggatcg cgccaccacc gttttgcatc gcagaatcgg
     1741 ccgcttctcc cagcgagtgg atgaacttga atacgcgctg agagatcgtc tgcgaagcgc
     1801 tctgttgagg aggagcacgg cgttgagcgg actcgaacag agacttcgtc ggatggatct
     1861 ccgtttgcga tttgccgccg cgcaccgccg ccttgccttc gcccacgccg aagcgattca
     1921 ggcgatgcgt tttcggttga ccgccgcgcg aggccgcttc gatcccctga atgcccaatt
     1981 ggctcaactc agcccgctac gcgtgctcga ccgcggatac gccattgtgc tggacgagcg
     2041 cggggaaatc ctgaaggatg ccgcacagac ttcgccgggc gcacaactcg agatccggct
     2101 gtcgcgaggg cgcgtcggcg cgcgcgtgac ggagcggaaa tagcccatcg ggcgcacaaa
     2161 ctctggccta tagtgaagaa gggtcccaac cgataccggg attgtgatgc tccgagatct
     2221 gcttctttac ctctccaccc agaagcatct tcgaaattgg gtggagacgt cgccgctggc
     2281 gggacgtctc acgtcgcggt tcatcgcggg aaacacgctt gaggaagcgc tggcggttgc
     2341 caccaggcta aacgccgaag ggctcctggt gacgctcgac catctgggag agaacgttac
     2401 ttcggaagtg gaggccaacg cctcgcggga tgcgtatctc caggctctcg gcaggatcgg
     2461 gcagttgggt ttgcgcgcca ccgtttcgat caagctgagt cagttcggcc tggccatttc
     2521 cgagcaattg tgccgcgaaa atgtcgccgt ggtggtggag caagccagcc ggatgaggac
     2581 ctgggtcgaa gtagacatgg aagcgagccg gtacgtcgac gccacgctcg cgctggtacg
     2641 tgaaatgcac tcgcggcacc aggccgtgcg cgccgtgata cagtcttacc tgcgtcgaag
     2701 cgagcacgat atcgaacagc tttgccgtga ccggatcccg gtcagggtct gcaagggagc
     2761 ttataaagaa gacgactcga tcgccttccc ggaaaaagaa caggtggacg ccaactatct
     2821 gcggctttca gaaattctgc tggagcgcgg cgtctacccg gggatggcca ctcacgatcc
     2881 gaaaatggtc cagggagttc tggattttgc atctaaacgg agcatttctc cggagcagtt
     2941 cgagttccag atgctatacg gcattcgacg cgacttgcag cggatgctga tccggcgcgg
     3001 cttccgtctc cggctctacg ttccatacgg aagcgcatgg tatccctatt ttatgcggag
     3061 gctggccgag cggccggcga atattctgtt tctggcgagg aatctctttc gctggtagga
     3121 acctgttagc ctttttcgga atccaatata gcggcacatg aaatactggg gctatcttgt
     3181 cgcgaaatcc accgtagcag cagttcttat ctacactttg tggcgcatgt tatgggtcgt
     3241 gatgccggag cccggctata tggtgtttca gcagacccgc cgcttcggcc aggatctctg
     3301 gtggacgacg gcgattctgg tgctgtggct cctgtcggct gcgctgatct atctcatcat
     3361 ttgggaccag cggcggcgct gccgcacctg tttgcgccgg ctccgcatgc cggtggaaac
     3421 cgggtcgtgg aagaatctgc tgctgattgg tccgccgcgc acggaataca tctgccctta
     3481 cgggcacggc acgttatccg tgcccgaact gcatatcacc ggaatggaaa gtccggactg
     3541 gcagccgcac gaagacattt gggcggaatt gtacggaacc gaggaaacca ggaaatgagg
     3601 cgtttcgctt cgtggaggat agccgccggg gcggttgtac tggcgtgtct gatcggcttt
     3661 cttgggctcc tggttccctc ctacctgcga aatcgggaat ttcagaattt tctcgaccgt
     3721 acggtgaact ccgaattggc gcaccggatg ccacccggtg cgctgcaggc gtccgtggcg
     3781 gacggcgccg gacgtatcgg attgccggtg gcgccggacc aggttcgcgt ccgtcagaca
     3841 gcggatgacc tgcgcatcga agtgcgctac gccgtacgcg ttgaaatccc gctctacacc
     3901 gttgacctcc acttccggcc ggccgcggtg agccggcgcc agtaaccgac actctacacg
     3961 tgagagaatc gaaacaggta attaagtggc tgtcagattt cagatcccca ggaaggcagg
     4021 gctggtccgc ctgctgctgc atcccgctgg taagttcgta gccgtctctc tcaccgtcgc
     4081 tcttatcgct ggaatcgggg cattcaccta ctactatgcg aagtattcga gactgatcga
     4141 acagaagctc agcgccggcc cgttcccgac tacgtccatg ctgtttgcgg ccccgaagat
     4201 cgtttccgtc ggtgatgaaa cggtcgcatc ggacatcgtg gcgctattgc ggcgcagcgg
     4261 atattccgaa tcgcgtacga accgcatggg ctggtaccgg cttaccagcg acggcatcga
     4321 agtttatccc ggcccggact cttatttccg ccaggaaccc ggagtgatcc gctttcaaag
     4381 cggcacgatc gcaagaatta tttcgctggc cgacaatacg gaacggacgc gctatcaact
     4441 cgaacccgag ttgatcacga atctattcga tcgcaagcgc gagaagcgcc gtattgtcgg
     4501 gttcagtgat attccgaggg tgctcatcaa cgcggtgatc tcgattgagg acaagcgatt
     4561 cttccaacac cctggcttcg atccgttccg cgtcatcaag tccgcctacg tggacttgaa
     4621 aaaaggttac ctcgctgaag gcgcgtccac actgaccatg caggtcgcgc gcacgttctg
     4681 gctgaatccg gataagaact ggagccgcaa gctggcggag acgctgattg ccttgcagct
     4741 cgaacaaaag ctcacgaaag aacagatctt cgaatactac gccaaccagg tggacctcgg
     4801 acggcgcggc agcttctcca tccgcggatt cggagaagcc gcccaggctt acttcggcaa
     4861 ggacatacag cagttgtcgc tttcggaagc cgcaacgctg gccggactcg tccagaggcc
     4921 aagctacacg aatccctatc gctggccgga gagagcgcgc gcgcggcgca acgtagtgct
     4981 ctccctgatg cgggaaaacg gatacatctc cgatgtggag tacgccgtgg cggctgcctc
     5041 gcccctcgtc atcaagaagg gcggtgtagg ctccggcgat gccgcctatt ttgtggacct
     5101 tgtgaatgac gagttgcagg gcgattttga aggccatgat ttccaggagg actcctaccg
     5161 cgtctacacc acgcttgacg tcgatttgca ggaggccgct gctgaagccg tacgcatcgg
     5221 catgcaggat gtcgacgcgc agttgcagag ccggcggaag aagaacccga actatccgca
     5281 ggcccaggtc gcgttggtag ccctcgatac ccagacgggt gagatcaaag ctttaatcgg
     5341 cgggcgcaac tacgggatga gtcagctcaa ccgggcgttg gcgaagcgtc aacccggttc
     5401 cgtgttcaag ccgttcgttt ataccgccgc gcttagcatg gggttcgacg acgcctcgcg
     5461 ggcggtgaca tcggccacaa cgctgctcga tgagccgacc accttctggt acaacgggca
     5521 ggcttacgag cccagcaact ataaacacca gttttatggg ttggtgaccg tgcggcaggc
     5581 cctggccaaa tcgctgaata tccccacggt aaagctggcc gaaatggcgg gctacgaacg
     5641 aattgtcgaa gtcgcccgca atgccggcat gaacctcgcc atccagccca cgcccgcggt
     5701 cgcgttaggc gcctatgaag tgacccccat cgagattgcc ggcgcgtaca cgatcttcgc
     5761 caaccagggg acctacgtga agccgtcctg gattaaatac atccgcgatt cttcagggcg
     5821 tgcgatctac gattacaagc cgaagcgcca acaggcgatc gatccacgcg tgtcgtatgt
     5881 catggagcag atgttggaag aggtgctccg caccggcacc ggcgcaggcg ttcgcgcccg
     5941 gggcttctct cttcccgctg ccggaaaaac cggtacgtca catgacggct ggttcgccgg
     6001 attcacctcc aagttgattt gtgccgtgtg ggtgggtttc gacgacaatc gcgagttgaa
     6061 cctggaaggc gcccgctctg ccctgcccat ttggacggag ttcatgaaga gagcgcacca
     6121 gttgcgcgaa taccggaacg ccagaccgtt ccccgcgccg gaaggcgtca tccaggtgga
     6181 gatcgatccg acaaccggac agctcgcgac gccgcaatgc ccgacggtcc ggacggactt
     6241 cttcatcgcc gggacgcagc cggttgaagt ctgccattat cacggcggca ccagccagat
     6301 cgccggatgg gagacaccgc cgcctgcgac tcccacgcct gttacattgc cgcccgccgc
     6361 gcccggcaac agcaacattg tccgcacaag ccgtggagcc ccgccgcagg tcacgacgaa
     6421 agcggagacg cccaccgaag agcgggagca accgaaaaaa cggggattct tcggccggat
     6481 ccgcgatata tttaggtagg tacaggaggc caatcatggt ttcccttact ttcgcagtgt
     6541 ttgccgccgg ttccgcttcc ttccttttag ccggccagag cgatggattg aagcaacccc
     6601 cggccattcc accttcggca gccgcttccg gagaggccca gccccgttca gccaatacga
     6661 cttcggagat gcgcggcgac gctctcatgg accggaaaaa ctaccaggag gcgattgtca
     6721 cctatcagga cgccgttcgc gaccaggcga tcctgatgaa caagatcgga atcgcctacc
     6781 accagttgac ggacctggac accgccaagc gctattacga aagagcgctt cagatcaatc
     6841 cgaactattc cgaggccatt aataatctcg gaaccgttta ctacgccaag aaagactacg
     6901 gtaaagcgga gcgcgaatac aaaaaagcac tcaaactgac tcccgaatcc gcctctattt
     6961 acagcaacct tggaaccgcc tactttgctc gcaaacgata cgacgatgcg atgcaagcgt
     7021 accagaaggc gctatcgctc gatccggagg ttttcgagca ccgtagcagc gccggtgtgc
     7081 tattgcagga acgaagcgtg gcagagcgcg caaaattcca cttctatatg gctaaaacct
     7141 acgccaaggc gggtgtgttc gatcgcgccc tgctgtacat gaggagagcg ctcgaagagg
     7201 gattcaccga acgcgaccgc ttccgccagg atcccgaatt cgcacagatg caggagatgc
     7261 cggaatttca acaattgctt cttctggaac cgcgtgtcct gtagtgtagt gtctcagaat
     7321 tggctttaca gatgccgagg aaccgctcgc tcacgctcgc ggctcagtac tgacttccga
     7381 gccgcgaccg tgagggagcg gtctgtctcc cgaggtgcgt tcctgttact gttcgctgtg
     7441 ctcctcgccg gctgctccag cagtccggaa aagagtggcg cgcagcgaac tgctattctt
     7501 ccttttgaaa atctgaccgg cgacaggtct ctggaatgga tgggccgtgc cgcatccgag
     7561 atcgtgatcg agagtctctc tggttcgccg tccatcacgg ctttacgcgt atcctccgca
     7621 aacgaagctt atgcggtacg agcggagagg cttgtggaag gctactatgc cctggtggat
     7681 ggcaagctgc ggctcaccgc ttccgttcgc gacatcgaac gcaaccggac catcgatagt
     7741 cctttcgccg agggtgcggt agcaagcggc attctgccgg tggcggccgg tatggcgcga
     7801 aacctgggtg gtgatacgaa ccggttcggc acccgcaaca atatcgcgat cgagaactgg
     7861 gcgaaaggtc tggcaggcac ggcgggagaa caaacctcgc gcctgttcga agccgccatt
     7921 gccgctgacc ccgatttcgg cgaagcttac gttcacttcg cagaatttct acttgggcgc
     7981 cccgacgtcg cggcggcccg gcggatcatc gagtcggcgc ggagcagaaa cctcggcgag
     8041 gtggagcgca cgcggctcga cgtcctgtcg gcatccatag ccggcgacgc gcgggcgcaa
     8101 ctcgaggccg tgcagcggct cgccaccttg actccgggcg atgccgtcct ctatcgaact
     8161 ctcggcaatt tgcagctttc ggcccatcgt tacggcgacg cctccaaggc cctcgcacgg
     8221 gccgcggaac tggatccggc agacccggat gcctggaacc tgctgggcta tgcccgggct
     8281 tacacgctag acctggaagg ggcgaaggcc gcgctcgaac aagccgcgaa gaccacgccc
     8341 gcagacgcca accctcagga ttcacttgga gatgttcatt tccttctggg ccggttccgg
     8401 gaagcggaga gctactatct cgcggcgcac gggaaaattc cttccttcct gggaagcgcc
     8461 acgctgctga aagcggcaca cgcgcgatgg atggccggag acctgccggg agctgacacg
     8521 attttccagc agtacacaga cgcgttacgg caggcgaagt cgcctctggc cgaattgcaa
     8581 gccgcgcggt gggagtatct aacaggccgc ttacagcaag gcgtggagcg cctggaaaaa
     8641 ttcgccgcga cggcgcaggg cgatgtgcgc gcactcgcat tttcgcaact cgcagtctgg
     8701 aagcttgcgg gcgggattcg aaccggcgcg gcgcaatcgg ctgccgaagc ggcgaaatcc
     8761 gcgagcagcc ccgcggcgcg cgtgaccgcc tatctggctg cggcggtgat tcgcggcggg
     8821 acggctggtg gcgatcaatc ggcaaacccc acgctccgcg attatgcctt ggcgtatagt
     8881 gcgctgcttg aaaaaagatt cgccgatgct gtgccgccgc tcgagcgcct ctacggccag
     8941 tcgtcgttca cgtccggaag gcgcatcgaa ctggtgctgg catgggctta tattgagacg
     9001 ggtgcattgg atcgcgcaaa aaagctgctt caaacgtatc cgctgcccgt gattcagccc
     9061 gaagagattt ttgcgtttct ggagtatccg cgcgttctgg agttacggaa aaaggtggga
     9121 ttaaaaaacc atgggagctg aagcccaccc cacgtatggg gcgggcctca acccgatgga
     9181 gaaagctaga agtagaactt cagagccagt tggccctgac gttcaccctg tgctccagtg
     9241 atttcaccga aggtcgcgcc ttgcacagcg cgcgccggcg ctgaaaactg cggtgtgttc
     9301 gtgaggttga agaactccgc acggaattcg aagcggtagc gttctccaat cgggaactgt
     9361 ttctggatcg agacatcgta ctgcttcaga cccggtcccc gttcggttcc gataccggtg
     9421 ctgccaaacg tgccggcggc gggaatccgg taagccgacg tgtcgaacca cctggtcccg
     9481 cgccctacgt cgccgacctt tccgccatct ccgatgcggt ccgctcgggc tccacgggag
     9541 ttggtgttgg aattgtcagg tccctgaatc gttagcggga atccgctgcg caaggtcagg
     9601 ataccgccca actgccagtt gccgagaatg cccatcaaca ccgggtgcat gtccgtgccg
     9661 aatcgtttgc cctgcccgaa cggcagctca taaacatgcg tgaacgtgag catgtgacgt
     9721 gcatcgaaat aggtcggtcc ccactccgct ttgcggtcat acaggttctg ccagtaggcg
     9781 gactgattgg cggattggcc gccctcgccg tagtagccga tggcgtcgct catgcctttg
     9841 gagaaggtgt aggaggtctg aaattccagg ccggaaccca tcctcttgcg ggccatcatc
     9901 tgaagtgcgt ggtattgctg gttgccgttg ctctcggttc cggaaatctg ggcaatatta
     9961 gccagttgcg ggttgccgct gagatagggg ctgggccgtg tcgtgccatc cggcatgagc
    10021 tggcgctgga aatacggcat cggaaccaca aggtgatggc ctttctggcc cacatagccg
    10081 gcggttaaaa ccagttgagc aggcaactga cgttcgaggg tcaggttcca ctgttgtgta
    10141 ttggcgggcc gaacgaacgg atcccatagc cggatattca cccggctgaa aggatcggcc
    10201 tgctggaggg ccgtcaagcc ctgcgatgca ttcgatagtg gaaggttcag gtcggaacgg
    10261 tcatacctgc cttcaaactc cgcattgaac ggagggttga atggcaggcg gaggttcgtg
    10321 cccgtgcctt ccatgaacga cgaaatggta taagcgcccc gcatgacggt ctgcgggagg
    10381 aaggtccagg caaatccgac gcgaggctgg aagtccttct tgaacggctc atagagggca
    10441 cggctgttcc cgtcctgtcc ggccagcaac agcctgccgg agaacggctc gaaattagcc
    10501 tgccggtcgt tcacctccac caacggcgtg tggtattccc accggaggcc gaggttcaga
    10561 gtcagcgtgt cgctgacgcg ccagtcgtcc tgcgcataca aaccatagat ggtcttgcga
    10621 tgtccccagg tgccggtgtt caggccccgg cccagtgtct caggcagacc caggaagaaa
    10681 tccgcatcgg cccagcctat tccgctcgaa tcggctcccg tgaatcgccc ggagaaacgg
    10741 atcaacccgg tgcggccgtt gttgccggca tagaacgtat tcatctgctg gcgaagaagc
    10801 tggccgccgg tcttgatcat gtgccggccg cggataattg tcaggttgtc cgcaaagtga
    10861 taggtcgtgt tcgcgaacag ttgctgcgtg ccgacattcg cgttcccgat gctgctcgtg
    10921 aagccattgg gaaattcgat ggagagcagg ccggggctgt tgactccggc gatgccgaac
    10981 tgctcgttga gatccggata tcctttgtta gctccgccgt tgtgcagagt gatccggttc
    11041 acgccggcgc gcgcctcgtt taccaccgtc gggctgaagg ttcgcgtcca gttcaagacg
    11101 ccggctttga acggagactc gttgaacgag ttgaaagtca gcgggaacgt gttaaagccg
    11161 ggattgttct gcatgctttg ggagtagcgg accgacaggt cgtcgttgct cgacagcttt
    11221 gcatccacct tgatatcgcc ctgatcgagg ttcaactggc tcgagttggc gttcgtttgg
    11281 ttgagccgca aatcggaatt caagggaagc ggatacaacg actggtcgcc gtagagagcc
    11341 ctcgcaacag ggctcatcat gctcatcgga atctggttat tggggaacgg ccgccgtctt
    11401 ccgtttgcat caaggtcgaa cgggttatac aactgcctgt tctgttcggt cagcaaacga
    11461 gagaaatcac cctgacggaa tccggccggt gcaacagtaa tagtctggat cgtcgcgggc
    11521 gtgggcttgc gcatgcccat atagtccgcg aagaagaaca gacggtccct tccgtcgaac
    11581 agctttggga tcgtaagcgg accgcccacg gtgccgccaa acgagttgta gcggagatac
    11641 gacctgggca agttgttcca gtttcgggcc cagttgttgg cattcaattt gtcattccgc
    11701 cagaaatgga acagcgatcc gtgcagatcg ttggttccgg atttcatgag gacgttgatg
    11761 atgccgccct ggaagttccc aaactcggcg gaggcgttgt ttgtaatcat acgcacctcg
    11821 gaaatcgcat cgaggttggg ttgataagag gtgaggttgt cggagacctg gttgttgtcg
    11881 atgccatcca gaagaaagtt gttcgcttcc ttccggttgc cattcacgta tgggcggcct
    11941 ccgccggttg ttcgctgatc gttattgaaa gcggccgggt tcgttgtggt cacgcccgga
    12001 ttcagtagcg tcaagatgat gtaattcctg ctgatcatcg gcgagttcgt cagccggttc
    12061 gtggtgatga cgccgccgac ttcggtacgg tccgtttgca gtagcggagc tgaacccgtc
    12121 acttcgatgg attccgtgac cgctccgatt tccagggcta catccacacg ggcacgctgg
    12181 gttgattcga ggacaatacc agggcgaacc gcggccttga atccttttgc ttcaatccga
    12241 agttcgtaat ttcccggcgg aactcggggg aagaagtaaa ttccctgttc gttcgttgtc
    12301 gtgttccagg tggtgcccct gtctacatcc ctggctgtaa tagcggcctg ggcgacggct
    12361 cctcctgttg cgtcggtgac ggctcccgtt ataccggcgc tgacttcctg gcaaaagctc
    12421 ggcgggatag caagactgag cgccatcagc acaaccagga atgttgaaaa tactgagact
    12481 ttcctcatgt ttgaactccc tttcaaactg cgagcggtga aataacggcg tgcggtactg
    12541 tgggtagacc ccctctagcc cttgccgtac tcgcatagct taacttgacc tgaaagtcaa
    12601 tctgaccgat ggtatgctga gattttgacg ggtgtcaatc catcaacaag tgaactgttg
    12661 atgcttccaa cagcgcaact gctggctgtc aggttcattt tcgccgtcgt aaaaacttga
    12721 tctcaaccac cagcgatgtc attataataa cctgaacaca tgatgtccag ccattctttt
    12781 tgaggcaagg taccatgcga agtcttgata aagctcttcc cactcctctt taccaccagt
    12841 tgaagacgat cctattgact gcaattgaag tagggcagtg gaagccggag gagcgttt
//
LOCUS       DASTMK010000553         7264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_30126, whole genome shotgun sequence.
ACCESSION   DASTMK010000553 DASTMK010000000
VERSION     DASTMK010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30126"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..961)
                     /locus_tag="VFQ79_22795"
     CDS             complement(77..961)
                     /locus_tag="VFQ79_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0142568.1"
                     /translation="MPAFSRRDFLRAGLSAGALGMTARFPLTAAKDAATEWVTLGKSG
                     VSVTRLAFGTGSNGGRVQRQLGQEEFTRLVRHAYDRGIRFFETAESYREMHQMLGIAL
                     KGIPRDTYRLMTKVTTRQGTDPQAKIDELRKLSNTEYFDIALLHWQHTATWPQDTVRW
                     QDGLQEAEVRKAVIAHGASVHGLPALRRFPGNQWLQVALFRTNHDGTKMDAEDYNTSG
                     PGDVTEVLSHVTKVGKEGIGVIGMKLIGEGAFTSREDRQKAMRFAFRRAGVQSVTVGY
                     KNTAEIDEAIENLNLALA"
     gene            complement(1043..2497)
                     /locus_tag="VFQ79_22800"
     CDS             complement(1043..2497)
                     /locus_tag="VFQ79_22800"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEU0142569.1"
                     /translation="MPQPSEIHSGRVAAAGLPRRSLTGLLTLVAIYAFIVLVLPRPAS
                     LTPEAWRLLGIFIATVAGLVLQPIPGGALVLIAVTIASVWGGLTIQQSLAGYSDPTVW
                     LVMAAFFISRALLNTGLAKRIALLFVRTVGKTSLGVSYALAASDMVLATIIPSNGARS
                     GGVILPIVRSISELYGSHPGATAGLLGSFLMTAVYQSICVTAAMFFTGQASNPLAARI
                     AADFGYNVTWSSWFLAGLVPGLCSLLIVPLVVMKINPPSIRKTPEAAAFAAGELRRMG
                     PMSGREKILFVIFISVCGLWVTSGWHNIDITVTALFGAAALLLTGILSWEDVKSERSA
                     WDIFIWYGGLLRLGKALNDAGVTAEFAKAVGNFFSGYTWLVLFVSALLIYFYAHYAFA
                     SITAHILAMYPPFLAVLVAQGAPVGLVVFAFACFTNLSAGLTNYGTTPSPMFFAHDYV
                     PLSKWWQVGFVVAAVNIAIWGTIGFGWWRLLGIW"
     gene            complement(2573..2956)
                     /locus_tag="VFQ79_22805"
     CDS             complement(2573..2956)
                     /locus_tag="VFQ79_22805"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU0142570.1"
                     /translation="METVMSKQEQIQRELEVLRAAVPEVRGVLVASTEGLPIAHSFVN
                     GADPQRLAAMSAAASSLGRRISESLNAGSLMEVSVAGSEGHIFVYSAGTRAVLAVVGP
                     AGANAGLIHLEARDAAKAILEIFGP"
     gene            3128..3634
                     /locus_tag="VFQ79_22810"
     CDS             3128..3634
                     /locus_tag="VFQ79_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142571.1"
                     /translation="MSTCLQCGRPMPLLHRLLGKQQFCSTAHRKLFQEEYNQSALLRL
                     QAVGEAMTRASTPAPPPPAPAREADPPQCGFVCLSPEAECSEPVETLPDLLPEVSIPA
                     PVILPPALARLAPPAQSTESASLTGSFEEPWMEFVDQSLIDAVVRSGGAVSEPESVPA
                     TASPGNGR"
     gene            complement(3606..5675)
                     /locus_tag="VFQ79_22815"
     CDS             complement(3606..5675)
                     /locus_tag="VFQ79_22815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142572.1"
                     /translation="MMLRCSVSLMLLAVLCQAQSPSKEKAAGPKPDLPVLYQSIVITA
                     SPVQPSLERRNEEVFRQTLFSRDDQLFHLLAAGIDAGQHEGGGKSIEVRRFGFNLDHG
                     GVNGGLKVLIDNVQQNQGTQGHGQGYLGSLKSVSPELVEEVSIINGPFSAEYGDFSGL
                     GVVHIRFRESLPDVWTARVQGGSFNTQRGFLAFSPELRDAESFIAYEGSRTDGPFLKP
                     LGYRRDNVTGNYTRRLSERRSVGLKWNGGRNDFYSSGQLPLDLVYGGRLDRFASLDPG
                     DGGRVCTGTVGLYYRADTAGGGVWKLDGFMSRSLFDLYSNFTFFLNDPVNGDGIQQHD
                     SRLQEGANLQYTQPHTWAGASGMLTAGGNFHANQINVGMYPRINRTPLGITLRAHAAV
                     TNGAGYVQEVFSLLNNRLQVGGGLRYDLFSFDLNDKVDPAGSMTRQQGNLQPKANIAF
                     TPSRRVPATLYFNYGRGISSLDARGIVEAPRGDLLATTDFYQVGTSHQFGRYAFTTDL
                     FWIDRSNELVYIPDDGSLEFAGPSRAYGFEAKASIGITRKLALEGGLTKVLNSYYRGT
                     DPREYIERAPHFVANGALTVAGWHGWSGSLRLRAINHYRLDPLDPTISAAGHTVLDFS
                     ASRRIRRGVEFNIAIDNLTDRAYFETQNYFESRLKDALPMERIHGTPGYPISVMVGLT
                     FRFRGKQ"
     gene            5789..7033
                     /locus_tag="VFQ79_22820"
     CDS             5789..7033
                     /locus_tag="VFQ79_22820"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0142573.1"
                     /translation="MSVTFTTRGALQKSVLRQANERLILNAVRNNPTISRSDLSRITG
                     LSPSSISFIVNRLVRKGVLSEEKLEKYSHVGRRPTALRLATDSMLAIALEIRTGGTRL
                     ALADLNGRFLRQKLIPWNSNPQVLAQRAHTAIRSMIDQAGSSRVLGVGVGVPGSVERF
                     SGRVIAAENLNWFNVDFGTPLRGRLQLPFYFENNARLSALAEKWFAEPGRKPLQDFIF
                     VATAGGLGTGVFTGGHLLEGATAVGGECGHIILHPDGLRCTCGNRGCWEQYASDKALC
                     RLYAGYAGLPDSTRCDAAGILDLARQGNEAATCALGEVADSLALGFANLLMVFNPEAI
                     IVGDFAADAWDLIEARIWTVLRSRAPRYALAPVRIVPATHASDSALLGGASVVFSHFF
                     TRFDNGGDSVRPGEVLLMQGAR"
BASE COUNT         1487 a         2252 c         2108 g         1417 t
ORIGIN      
        1 atccggatag atccatcaga acgcttttga ggccgctctt cgccggcgaa gccgcacccg
       61 cacagccgcc cgccgcttat gccagcgcga gattgagatt ttcgatcgct tcgtcgatct
      121 cggccgtgtt cttgtagccg acagtcacgg actgcacgcc agcccggcga aacgcaaatc
      181 gcatcgcctt ctggcgatct tcgcggctcg tgaatgcgcc ttcgccgatc aacttcatac
      241 cgatgacgcc gatgccttct ttgccgacct tcgtgacatg cgacaacact tccgtaacgt
      301 caccggggcc gctcgtgttg tagtcttcgg catccatctt cgtgccgtcg tgattcgtgc
      361 ggaacagcgc aacctgtagc cactgattgc cgggaaaccg gcgcaacgcg ggcaggccat
      421 gaaccgatgc gccatgggct attaccgcct ttctcacctc ggcttcctgg agaccatcct
      481 gccagcgaac cgtgtcctga ggccatgtcg ccgtgtgctg ccaatgaagc agcgcgatgt
      541 cgaagtactc tgtgttggac agtttgcgaa gttcgtcgat cttcgcctgt ggatctgtgc
      601 cctggcgcgt ggtcaccttc gtcatcagcc ggtacgtgtc gcgcggaatg cctttcaagg
      661 cgatgcccag catctgatgc atctccctgt aggattcggc tgtctcgaag aaccgaatgc
      721 cgcgatcgta ggcatggcgg accagccgcg taaactcctc ctggcccaac tggcgctgca
      781 cacgtccgcc attactgccg gttccgaacg caagccgggt tacactcacc ccggacttgc
      841 cgagagttac ccactccgta gctgcgtcct tcgccgccgt gagcggaaac ctcgccgtca
      901 ttcccagggc accggcagaa agcccggctc gtagaaaatc ccgtcgtgaa aaagcaggca
      961 tcgtccctcc ttccctccaa tggccaaatc ttagcatgaa agccttggcc tggctgaacg
     1021 aatacaaccg gcgccgcagc ctctaccaga ttccgagaag gcgccaccat ccgaacccga
     1081 tcgtccccca gatcgcgata ttcacagccg ccacaacgaa gcccacttgc caccatttgc
     1141 tgagcggcac atagtcgtga gcaaagaaca tggggctcgg cgtggtgccg tagttggtta
     1201 gcccggcgga gaggttcgtg aaacaggcga aggcgaacac gaccagaccc acgggcgcac
     1261 cctgtgccac cagcactgcc agaaacggcg ggtacatcgc aagaatatgc gctgtaatgc
     1321 tggcaaaagc atagtgtgca tagaaataga tcaggagcgc gctgacgaat aacaccaacc
     1381 atgtatagcc gctgaaaaag ttcccgacgg ctttcgcaaa ctccgccgtc actccggcgt
     1441 cgttcaaggc ctttcccagc cgcagcaaac ccccgtacca gatgaagatg tcccaggcgg
     1501 accgctcgct ctttacgtcc tcccaactga gaataccggt cagcagcagg gccgccgctc
     1561 cgaacagcgc ggtcaccgtg atgtcgatgt tgtgccaacc cgacgtcacc cacaggccgc
     1621 acaccgatat gaaaatgacg aacaggatct tctcccgccc gctcatcggc cccatccggc
     1681 ggagctcgcc cgcggcgaat gccgccgcct ccggagtttt tcggatggac ggcggattga
     1741 tcttcatgac caccagaggc acgatgagca gcgagcacaa tcccggaaca agaccggcca
     1801 ggaaccaact ggaccacgtc acgttgtaac cgaaatcggc ggcgatccgg gccgccagcg
     1861 gattactggc ctggcccgtg aaaaacatgg cggccgtcac acagatgctc tggtacaccg
     1921 cggtcatcag gaacgagccc agcagccccg ctgtcgcgcc ggggtgcgat ccgtatagct
     1981 ccgagatgga gcggacgata ggcagaatca cgccgccgga gcgggcgccg ttcgatggaa
     2041 tgatggtggc aagcaccatg tccgacgccg ccagtgcgta cgatacccca agcgaggttt
     2101 tcccaaccgt gcggacgaac agcaatgcta tgcgcttcgc caatcccgtg ttcagcaagg
     2161 cacgcgagat gaagaacgcc gccatcacca gccatacggt gggatcggag tacccggcaa
     2221 gagactgctg gattgtgaga ccgccccaca cggaggcgat cgttacggcg atgagaacga
     2281 gcgctccacc aggaatgggc tgaagcacca gccccgccac cgtcgcaatg aaaatgccca
     2341 ggagacgcca ggcttcagga gtgagagaag ccggccgtgg cagcaccagc acaatgaacg
     2401 cataaatggc aacaagtgtg agcagcccgg tcaggctgcg ccgtgggaga cctgccgccg
     2461 ctacacggcc ggagtggatc tcggaaggtt ggggcattga tgaggtaagg acaattctaa
     2521 cagcctacga acgattattc gccggatgcg aagccttacc tcatcgggaa aatcaagggc
     2581 cgaaaatctc caggattgct tttgccgcgt cgcgggcctc caggtgaatc aggccggcat
     2641 tggctccggc cggtccgaca acggccagca ccgccctggt tccagccgag tagacaaaga
     2701 tgtggccctc acttcccgca accgacacct ccatcagcga gccggcattc aggctctcgc
     2761 tgatccggcg gcccagactc gaagccgccg ccgacatagc agccaggcgc tgcgggtcgg
     2821 cgccgttcac gaacgaatgc gcgatcggta agccttccgt agatgccacc agtacgccgc
     2881 gcacctcggg caccgctgcg cgcaacactt ccaactctct ttgtatctgc tcctgcttcg
     2941 acatcacggt ctccattttc gccagtgcag cagcttgcag ccgctataag gaagatccgc
     3001 tgtaaggact ctgcttcccg taggggattt aaccgggatc ggcgattggt agaccttaag
     3061 acagccataa aagctcacta ccgcatggaa ttcgcaatcc ggaccccaca caataaaaag
     3121 tggggatatg agcacctgcc ttcagtgcgg gcgaccgatg ccgctgctgc accggttgct
     3181 gggcaaacaa cagttctgtt ccacagccca tcgcaagctt tttcaggagg aatacaacca
     3241 atcggcgttg ttgcgtttgc aggctgtcgg ggaagctatg acgcgcgcat ccaccccggc
     3301 gcccccgcct cccgcgcctg cgagagaggc cgatcccccg caatgcggtt tcgtgtgcct
     3361 gtccccggag gctgagtgta gcgagccggt agagacgctt ccggacttgt tgccggaagt
     3421 aagcattccg gcgcctgtca ttcttcctcc tgcgctcgca cggctcgcac cgccagcgca
     3481 gtccacggaa agcgccagcc tgaccggctc tttcgaggag ccctggatgg agttcgtcga
     3541 ccagagcctg atcgatgcgg tcgtcaggag cggtggtgcc gtttcagagc ctgagtctgt
     3601 cccggctact gcttcccccg gaaacggaag gtgagcccca ccattaccga gatagggtag
     3661 ccgggcgtgc cgtgaatcct ttccatgggt agcgcgtctt tcaacctcga ttcgaaatag
     3721 ttctgcgtct cgaagtaggc tctgtcggtg agattgtcga tggctatgtt gaattcgaca
     3781 ccccggcgga tgcggcgcga cgcgctgaaa tccaacacag tatggccagc ggcactgatg
     3841 gtggggtcca acggatcgag ccggtagtgg ttaatcgcgc gcaggcggag cgatccgctc
     3901 catccatgcc accccgccac cgttaacgcc ccgtttgcga caaaatgcgg agcacgctcg
     3961 atgtactccc gcggatcggt cccccggtag taagagttga ggactttcgt gaggccgcct
     4021 tcgagtgcca gctttcgcgt gatcccgatg gatgccttcg cctcgaagcc atatgcccgg
     4081 ctgggcccgg caaattcgag cgagccatca tcgggtatgt agacgagttc gttcgaccgg
     4141 tcgatccaga acaggtcggt ggtaaacgcg taccggccga actggtgaga ggttcccacc
     4201 tgatagaaat cggttgtggc gagcagatct ccacgcggag cctccacaat gccccgcgca
     4261 tccagcgacg agattccacg gccgtaattg aaatacaggg ttgccggcac tctgcggctg
     4321 ggggtaaacg cgatattcgc cttcggctgt agatttccct gctgccgcgt catgctcccg
     4381 gccgggtcga ctttgtcgtt caggtcgaaa gaaaacagat cgtagcgcag tccgccgcct
     4441 acctgaaggc ggttgttcag caggctgaat acctcctgca catagccggc gccgttggta
     4501 acggccgcat gcgcgcgcag ggtgattccc agaggtgtgc ggttgatgcg cgggtacatc
     4561 ccgacgttga tctggttggc gtggaagttg ccgccggctg tgagcatacc ggaggcgccg
     4621 gcccaggtgt gcggctgtgt gtattgcaga ttggcacctt cctgcaaccg cgagtcgtgc
     4681 tgctggatgc cgtcgccgtt taccggatcg ttgaggaaga aagtgaagtt cgaatagaga
     4741 tcgaacagag accggctcat gaagccgtcg agtttccaaa ccccgccccc ggccgtgtct
     4801 gcccggtaat agagtcccac cgtgcctgtg cacacacgtc ctccgtcgcc agggtcgagg
     4861 gaggcaaagc ggtccagccg tcccccatag acgaggtcca gcggcaattg tcccgacgaa
     4921 tagaaatcgt tgcggccgcc attccacttc aatcccacag atcgccgttc agaaagccgc
     4981 cgcgtatagt tgccggtcac attgtcgcgc cggtaaccca ggggtttgag aaaaggaccg
     5041 tccgtacggg agccctcata cgcgatgaac gattcggcat cccgcagttc cggactgaag
     5101 gccaggaagc cgcgctgcgt gttgaaggag cctccctgca cccgtgccgt ccaaacgtcg
     5161 ggcagcgatt cccggaagcg gatgtgcact acgcccaggc cgctgaagtc gccgtactcg
     5221 gcgctgaacg gcccgtttat gatgctgact tcctctacga gttccggact tacggacttg
     5281 agcgagccca ggtacccctg gccgtggccc tgcgttccct ggttctgctg cacgttgtct
     5341 atcaggacct tcagaccgcc gttgacgcct ccatgatcga gattgaagcc aaaccggcga
     5401 acctcgatcg acttgccccc accttcgtgc tggcccgcgt cgatgcccgc cgcaagcagg
     5461 tgaaatagct ggtcgtcgcg agaaaaaaga gtctgccgga acacctcttc gttccgccgt
     5521 tccagggaag gttggacggg cgacgccgtg atgacgatgc tctgatacag taccgggagg
     5581 tcgggcttcg gcccagccgc cttttcctta gacggcgact gagcctggca cagcaccgcg
     5641 agaagcatga gcgaaaccga acagcgtagc atcatttttt tttacgtaat actaccataa
     5701 atacgctgga gtctcttcaa atggatctat aaaaaaagcc tattgacaat ttttttacaa
     5761 actggaataa tttgggactg cggcatccat gtccgtcact ttcacgaccc gcggagcgct
     5821 tcagaaatca gtcctacggc aggcaaatga gcggctcatc ctcaatgccg tccggaataa
     5881 ccccaccatc tcgaggtccg atctttcgcg catcaccgga ctttccccca gttccatcag
     5941 cttcatcgtg aaccgactgg ttcgcaaagg ggtactttcc gaagaaaagc tcgagaagta
     6001 ctcgcacgtg ggccggcggc ccacggcgtt gcgcctggct acggattcga tgctcgccat
     6061 cgctctggaa atcagaaccg gcggtaccag gctggcgctg gcggatctca acggccgctt
     6121 tctgcggcag aaactgatcc cctggaattc caatccccag gtgttggcgc agcgggccca
     6181 tacggccata cggagcatga tcgaccaggc aggctcaagc cgtgtgctcg gggttggcgt
     6241 tggagttccc ggttcggtcg agcggttcag cggacgggtg attgcggccg aaaatctgaa
     6301 ctggttcaac gtggatttcg gaacgccgct gcggggccgg ctgcaactcc cgttttactt
     6361 tgagaacaac gcccgtctct ctgcgctcgc ggaaaagtgg ttcgctgagc ctggccgcaa
     6421 accgctgcag gactttattt tcgttgcgac cgccggcggt cttggaacgg gagtattcac
     6481 tggaggccac ttgctcgaag gcgccacggc ggtcggagga gaatgcgggc atatcattct
     6541 gcacccggac ggtctccgat gcacttgcgg gaaccgcggc tgctgggaac agtatgcttc
     6601 cgacaaagcg ctgtgccgcc tgtatgccgg atatgccggg ctaccggatt caacgcgctg
     6661 tgatgccgcc gggatactgg atctcgcgcg gcaaggcaat gaggcggcca catgtgcctt
     6721 gggcgaggtc gccgattctc tggctctcgg atttgcaaac ctgctgatgg ttttcaatcc
     6781 ggaggccatc atagtaggcg actttgccgc ggatgcctgg gacttgatcg aagcgcggat
     6841 ctggactgtt ctgagaagcc gcgctccacg atatgcgctg gcgccagtcc ggatcgtccc
     6901 agccactcat gcctccgatt cggccctgct gggaggcgca tccgtggtgt tctcccattt
     6961 ttttacacgc ttcgacaacg gtggcgattc tgttcgcccc ggcgaggtcc tcctgatgca
     7021 gggcgcccgc tgatccggtt tgggctctta tgctcccgtt atttacatca gaccccttga
     7081 cgccggcatt tttaatattt acagtattca aaataattga actggatggg agacgaaccc
     7141 ctgttacccc gaacggagga acgaaacgtc aacagcggcc aacggaggtt aggtaatgaa
     7201 gtatctgggt aactttcgaa aagtctgcct cgctccggcg ctcgcaatgt gctttgcggc
     7261 gctt
//
LOCUS       DASTMK010000554        16169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_316929, whole genome shotgun sequence.
ACCESSION   DASTMK010000554 DASTMK010000000
VERSION     DASTMK010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16169
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316929"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..810
                     /locus_tag="VFQ79_22825"
     CDS             31..810
                     /locus_tag="VFQ79_22825"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0142574.1"
                     /translation="MAEERLQKIISRAGIASRRKAEELISAGKVTVNGATITELGSKA
                     DAEHDSIKVNGRLLRPPSRLLYLALYKPDSCVTTVSDPQGRRTVMDLLRGVKERVYPV
                     GRLDYHSEGLLLLTNDGDFANGITSAKSQVPKTYVVKVNGQLSLAQEEQFRTGIPLGG
                     KRTAPAGLKLIRPGQNPWYEVRLIEGRQNQIREMFKHLGFLVEKLRRVRIGFLELGSL
                     QPGEFRMLTPAEVARFQKLLGIKDENSAHARNGNSRRHPGK"
     gene            770..1192
                     /locus_tag="VFQ79_22830"
     CDS             770..1192
                     /locus_tag="VFQ79_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0142575.1"
                     /translation="MQETGIVAGILENSRTIAVVGLSNNRYRPAYGVSEYMQRAGYRI
                     VPVNPNEMEVLGEKAYPSLDDVPEPVDIVNVFRRSEYVPDIVEAAIRKKAKAVWMQEG
                     VSHQAAAERAGAAGLAVVMDRCILKEHRRISRDNTSAV"
     gene            1341..3485
                     /locus_tag="VFQ79_22835"
     CDS             1341..3485
                     /locus_tag="VFQ79_22835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU0142576.1"
                     /translation="MKDLGHSPVSPPRRVEFVTTRDARLLVLGLLGLFVLLTGIFGFV
                     SSKYSTLVDRRLLSGPFSDTYNIYAEAGYDGKPRLVTNVAEQGREKRRLVSFQEIPAV
                     LVQAVTSAEDKRFFEHPGFDLARIAKAAWVDLKAGRKRQGASTLTMQLARSLWLDTDK
                     KWRRKLSELMITLMLEDKLTKQQIFEYYANQIYLGRRGTYSIHGFGEAAYAFFGKDLK
                     DVNLPEAALLAGVAQRPGYSDPRRYAERARLRRNVVLSLMKENGHIGEEQYRRAAAAP
                     VEIANAPAQAQEAPYFLDQVNNELSERFASEDTGQLNIYTTLDMRLQRAATEAVDAGM
                     KHVDKELKRQKIDGVAQVALIALDPHTGEVKAIVGGRDYSQSQLNRALARRQPGSVFK
                     PFVYAAALSTTSRGSGEIFTPATALVDEPTTFNFGRTAYEPGNYGGNFNGTVTLRSAL
                     VRSLNVPTVKLAEMVGYKNVVALARNAGLNDEIRATPSMALGSYDATPLEIAGAYTVF
                     ANSGTFVRPTLIREARTPAGKVLYSHSPKRSPVLNPQINYLMVTMLGDVMNWGTGSRV
                     GALGFTQPSYGKTGTSHDGWFAGFTNSLLCVVWVGFDDYRELKLEGSRSALPVWVEFM
                     KRASKIAGPARSIEAPAGLTSEIVCADSGKLPGPFCPKVREEIFLPGTEPVDYCDLDA
                     GPQWPGAEGAGTHSIASGPPSAATALPASYER"
     gene            complement(3489..4682)
                     /locus_tag="VFQ79_22840"
     CDS             complement(3489..4682)
                     /locus_tag="VFQ79_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0142577.1"
                     /translation="MSFSDPGEFYRIEKLPPYVFAVVNEMKARLRSEQKDVVDFGMGN
                     PDGPTPRAVVNKLVEAARNSRNHRYSLSKGIPKLRQEIVKRYKRNYGVDLDPDVEAIA
                     TIGAKDALAHLLFAVIGPGDPVVSPNPAYPIHQYGVAMADGMPCMVPMPNPGAFLNGL
                     EQLYSSLSKKPKLILISFPHNPTTQCVDLDFHREIIRLAAHHGTMVVHDFAYADLGFD
                     GYQPPSILQVEGAKEHAVEIFSLSKSYNMAGWRVGFCLGNRRMIAALARIKSYLDYGI
                     FQPIQIAAIIALRECEEDTHQIRTLYQNRRDLLIEGLCRAGWPVSPPRGSMFVWAPIP
                     EKFRHLGSLEFSKQLLEKALVAVSPGIGFGPLGEGFVRFALIENHHRSRQAIRSIKQF
                     LNGKC"
     gene            4713..4925
                     /locus_tag="VFQ79_22845"
     CDS             4713..4925
                     /locus_tag="VFQ79_22845"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEU0142578.1"
                     /translation="MSVLSPGALLNAAIFAIVGLVLFAVVLSMIEKFTPKLIWTEVIE
                     KQNLAAAVLAGAIAIALALIVSSAVH"
     gene            complement(4922..5821)
                     /locus_tag="VFQ79_22850"
     CDS             complement(4922..5821)
                     /locus_tag="VFQ79_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEU0142579.1"
                     /translation="MNITVTGASGFLGTRLLERLTTLGHSVHGLARKPPKGQAKRVAF
                     SAWDVTDGTRPAEAFEKAEAVVHLAGEPIAQRWTPEVKRRIRESRSEGTRNLVSRIEV
                     TGKQVRTLVSASAIGYYGSRGDDLLTEASPPGTGFLADICAEWERAAEQARAAGIRVV
                     TIRNGIVLGREGGALRQMLTPFRLGVGGKLGDGQQWMSWIHADDLVELIRFAVEEPRL
                     SGPVNGTTPNPVRNIDFTRALAKALHRPAFLPVPVLALKLLYGEMAEVLTSSQRVLPH
                     AAVAAGFRFQYPFIDDAFEAIFG"
     gene            5852..6940
                     /locus_tag="VFQ79_22855"
     CDS             5852..6940
                     /locus_tag="VFQ79_22855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0142580.1"
                     /translation="MRAWNRAKLLWGYLRRKSELGGLPVEFIVETTAKCNLYCPMCPR
                     ETHKQPKADMADEIFERLVHESGKTSEHMMLIGLGEPFMDRRIFDRIEYCERHGISTL
                     LSTNGTFLDEKTSERLLGSTLEHITLSFDGATKESYEYYRKGAKFEKVRDNFVRFARM
                     KHERKSKMQIVVQMVRMERNWDEVEEFTRFWRAVPGVDQVRIKADETNVLDPASSHAA
                     EDWKYPCHYLWRGPLYVKQNGDVYPCCQSYMLDGAPVGNIETESLGSIWNSGEMQRMR
                     RLHVEGRAGKIDICSRCCTTIPHPALVAGSLLLHGRTVRRLLPLVERLAYFSKMPARL
                     LRPPKPPNRRPELVQISGPPGKGSPADS"
     gene            complement(6873..8438)
                     /locus_tag="VFQ79_22860"
     CDS             complement(6873..8438)
                     /locus_tag="VFQ79_22860"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU0142581.1"
                     /translation="MDHILSIVLFTPLIGLLVLLFIPSSNSKAVKLWANIVSFAGFLV
                     CLPLIFGFDRTKDFQFVEKADWIPSLGASYHLGIDGFSLLLVVLTGLLGFLAILSSWN
                     AIQTRLKEYYAYFLLLQVGMLGVFMALDFLLFFVFWELVLVPMYFIIGIWGGPRRVYA
                     AIKFIIYTLIGSVLMLLGILMLYFEHYSQFQVLTFNIADLLQTDLSGTVQWWVFWAFF
                     LGFAVKVPMWPFHTWLPDAHVEAPTAGSVILASVLLKMGTYGFLRFSLPLLPDAAKNP
                     TIVNIMAVLSIIAIVYGALVSLMQKDWKKLVAYSSVSHLGFCTLGIFALNPAGIAGSI
                     LQQINHGISTGMLFLIVGVVYERRHTREISEYGGLLRIMPVFAIVFLITALSSMGMPP
                     LNGFIGEITILQGVFQMSFNWAFWCVVGILLGAAYLIWLYQRTVLGEVSEKNARLKDL
                     SLREMAIFAPLLVWAFWIGLYPRPFFEVLERPVAQIVERVRPGYYAEHNLRNPLASPS
                     PAARISAPVPAGD"
     gene            complement(8502..10358)
                     /locus_tag="VFQ79_22865"
     CDS             complement(8502..10358)
                     /locus_tag="VFQ79_22865"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0142582.1"
                     /translation="MPVSRREFIGHTLAASSLRGAQTGAPRPNILWLSNEDMSPLLGC
                     YGDAHAITPNLDALAKRSVRYAHAYTTAGVCAPSRSCVITGVYQSTLGSQNMRSSAPL
                     PDFVKCFPEYLRRADYYCTNNQKTDYNFPVPEGAWDESSGKAHYHNRRAGQPFFAVFN
                     NVATHESQVRAPAEAFAKQTARLAPRDRQDPAKLTLPPYYPDTPTVRRQWANHYELNT
                     AMDYWAGDLLRQLEDDGMADDTIVFYWSDHGSGLPRAKRWLYESSTHVPLIVHIPEKF
                     RRDRQGTPGSVSEELISFVDLAPTVLNLAGVPIPGHMQGRAFLGPKLQPPRQYVFGMR
                     DRMDERYDIIRSVRDKRYRYIRNYEAYKPYSQFLSYCEQSPVMQELRRVKAEGTLPPA
                     AAHFMADRKPVEELYEAESDPHETKNLAESVEHRAVLERLRKAHLTWMEEARDLALVP
                     EPELEKADRELGSRYAILRRPENKDLPRRLRQMVELGEQGKPALAKLVAALGDAEPGV
                     RYWAATGIGNLGENAATAEEALRKALQDSSGMVRVAAARALWKAGKAEAALPVLTAEL
                     KGSEQWVRLYAVIVLDEMGSVARSAVQSLQDALKDENDYVRRVATHAVEALA"
     gene            complement(10377..10655)
                     /locus_tag="VFQ79_22870"
     CDS             complement(10377..10655)
                     /locus_tag="VFQ79_22870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142583.1"
                     /translation="MPVLIRVRNRKAILRRGEWVCADGRTEALLNSVTELWIHETGGP
                     ALSDGDPEKAVAEYVAAQTEGRVLLHIPARPRSSRQLYLGRRQLTLQF"
     gene            complement(10703..11533)
                     /locus_tag="VFQ79_22875"
     CDS             complement(10703..11533)
                     /locus_tag="VFQ79_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142584.1"
                     /translation="MANVDNGFRNDARVLYDAGAAWIVPVTQTRLFGSWSTGYKLNKA
                     FYLWWGGGEFIRRNPATGLDPSTTETTEIGLEQPISFGGNRTGRVRVSVFSARESDLF
                     NFGDSHSGLPYYDDARTRGAELWTEWRIWRLRPFASFTWLRSYRDASTNPEAADVDLR
                     FAPLPNYSAGFGSHVDLHPRLILSIYGYYDDGGVSQEMLNDEVMVTRYGSFSKINASL
                     AWVASSRWGVFSRIENLFHRRDLGFDRTTIGPDGSASRTAGTQRDPGLVASGGVELRF
                     "
     gene            complement(11566..13011)
                     /locus_tag="VFQ79_22880"
     CDS             complement(11566..13011)
                     /locus_tag="VFQ79_22880"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0142585.1"
                     /translation="MAETRYALISRWCLLRAVCLAVLLSGAMPAFAEERTAVEGTVVD
                     SQEKPVSGAEVALEFPDGTVETRHADPEGRFRFEDVKPGPATLRVTAKGFEVLRQAIA
                     GKDAAATLRLHLRIAPLRTGVTVTDSHPADLRMQVDEIYNRNKSISTLNGLDLVNFNP
                     VANYSALRLLPGVMTAAYGGRDRFSLPTHIRGGHAWGIVETIDDYPAINITPVNAEDG
                     GYTAGFSSVIPSIAVESLSVATGGLGVSYGQASGGVVRNQIRRGSVGNITSSVRLEML
                     SLGEGIVMGDTGGGFGRVDYYVAGQSSLADYGTAYDTFPRPIEGLRLGSGVAKVGLRV
                     TRSSRWETMYAGGGERHDYFQNATIAGQPARREFHTDKSNHFLATRYDWRRSEDLGFG
                     LGVTQNWFHEDRIEDFAAGVPVGLSRRNRPQRATRAFSTFTGALPLRTPFPIRHREAA
                     ISPGTGSGISPPRPSASLSANRRATGAIPLQ"
     gene            complement(13256..14452)
                     /locus_tag="VFQ79_22885"
     CDS             complement(13256..14452)
                     /locus_tag="VFQ79_22885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0142586.1"
                     /translation="MKDLQKILISHGDSNAVHQIRDALAGGRYELEEAGTAAETIEKL
                     AEFHPDVVLADAGLEGMDGLGLMRHLASEPRPPLVVLITAKGLERTAVQALREGAHNF
                     ILWPFDAAELQSVTHNAVEQRRLLRENESYEEELQHALMRLKNSQAALVQAEKLGSMG
                     KLVAGVAHELNTPLGALRSSTDTIERASQRLLDWARSQEPEIAGQVQPFIDILSTTAA
                     QSKNACDRITGIVTNLRQFAQLDRADLQRAHVHDSLESTLALLNHEFNGRVEVVKEFG
                     DVPEIECSPRQLNQLFMNLLLNANEAIRQSGRAQGVVRLRTWQQDDWLKIEISDNGVG
                     ISEKDMERIFDPGFTTKGGVKVGTGLGLPISYQIVKEHHGRIEVVSRPGQGSRFTIVL
                     PLEQGL"
     gene            complement(14479..15885)
                     /locus_tag="VFQ79_22890"
     CDS             complement(14479..15885)
                     /locus_tag="VFQ79_22890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0142587.1"
                     /translation="MNLQALRKCLLPNDAEQDEGFRQEILRLSHQCLKIIAAVQAGAA
                     VAITLAHFAVGRGQGMVGVRLLQSGIMIALGGFLLAMHHLPAAYARSRTIAVLTAFAS
                     GSVATWFSLWIAPRAPYMDNFIPSQVTLVVLVAIAAIPLKPKHTLALCLSLAGFYAWA
                     ASRPESFSLPPATTLDPTYLFFFLILSVLAVSLTAVAYEQRHRNYQAYQETLTACDNL
                     RVAQQRMLSSEHSATLGRIAAGLSHEFNSPLGALTSAIDTLLLLSSKQVSATPVEQQR
                     YVSLQAELRKTIGESSERIRKTILRMQRFTNLDQAEIRRIDINHLLEDVAALVRPGMD
                     SRHQIVLDLKPVPRVICRPQQLSAAFATLLNNAMEAVNGNGRIAIGTLPRGAEIEVQI
                     GDNGRGVMPEDMAAIFEPGFRETAGRISSANWGMFSARQIVREHGGEISIVSTPGEGT
                     TVSVVLPGEASPRSIRIS"
     gene            complement(15928..>16169)
                     /locus_tag="VFQ79_22895"
     CDS             complement(15928..>16169)
                     /locus_tag="VFQ79_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142588.1"
                     /translation="LEIEKQLLDLHDRGRPVLCHAAIVRAGRGDIPGALDLLERSTGN
                     QEGPLPSILVDQRMEPLRPEPRFQALVDRVQPFRR"
BASE COUNT         3194 a         5015 c         4822 g         3138 t
ORIGIN      
        1 tgaccgatcc ggaaccggag aagacagccg atggctgagg agcgtctcca gaagatcatt
       61 tcgcgtgccg gcatcgccag ccgccgcaaa gccgaggagt tgatctccgc cggaaaggtg
      121 acggtgaacg gcgccaccat cacggaattg ggcagcaagg cggatgcgga gcacgacagc
      181 atcaaggtga acggccgcct gttacgtccg ccgtcgcgcc tgctctacct ggcgctgtat
      241 aaacccgata gttgcgtgac cacggtcagc gatccgcaag ggcggcgcac ggtcatggac
      301 ctgcttcgcg gcgtgaagga gcgtgtgtac ccggtaggcc gcctcgatta tcacagcgag
      361 ggcctgctgc tgttgaccaa cgatggcgac tttgccaacg gcatcacatc ggcgaaaagc
      421 caggtgccga aaacctacgt cgtgaaggtg aacgggcaac tctccctcgc gcaggaagag
      481 cagtttcgta ccggcattcc actcggtggc aagcgcacgg cgcctgccgg attgaaactc
      541 attcggcccg gacagaatcc atggtatgaa gtccggctga tcgagggaag gcaaaatcag
      601 attcgcgaga tgttcaagca cctgggtttc ctggtcgaga aactgaggcg cgtccgcatt
      661 gggtttctgg agcttgggtc gctccagccg ggtgaatttc gcatgctgac tccggcggag
      721 gtcgctcgct tccaaaaact tctgggtatc aaagatgaga attccgcaca tgcaagaaac
      781 gggaatagtc gccggcatcc tggaaaatag ccgcacaatc gccgtggtgg gcctgtcgaa
      841 taaccgctac cgccccgctt acggtgtatc cgagtacatg cagagggccg gataccggat
      901 tgtgccggtg aatcctaacg aaatggaagt gctcggcgaa aaagcctatc catcgctgga
      961 tgatgtccct gaaccggtgg atatcgtgaa tgtattccgg cgctccgaat acgtgcccga
     1021 tatcgtggaa gccgccatcc ggaagaaagc caaagcggtt tggatgcagg aaggcgtctc
     1081 tcaccaggcg gccgcggagc gggccggcgc tgccggtctt gccgtggtga tggaccgctg
     1141 cattctcaag gaacaccgtc ggatatctcg cgacaacact tccgcagtgt aaaaaagagc
     1201 ttgttgaaag cgcatcgcag ccaccgctcc ctcacggtca cggctccata acgtagtgat
     1261 tctactgagc cgcgcgcgcc agcaagcggt tttccggcgg agtcgcagaa tggaaaccca
     1321 gttgcacgta ccagggttgc atgaaagact tgggccattc gcccgtttcg ccgccgcggc
     1381 gcgtcgagtt cgtcacaact cgggacgccc gcctcctggt gctcggtctg ctcggcttgt
     1441 tcgttctcct cactggcatc ttcgggtttg tttcctcgaa atattcaacg ctcgtcgatc
     1501 ggcgtctttt gtccggccct ttttcggata cctacaacat ctacgcggag gccggctacg
     1561 acggcaagcc gcgtctcgtc acgaatgtgg ccgaacaagg ccgcgaaaaa cgccgcctgg
     1621 tgtccttcca ggaaattccc gctgtgttgg tgcaggcagt gacctccgcc gaggacaagc
     1681 ggttctttga gcatcccgga ttcgatctcg cgcgcattgc caaggccgcg tgggtcgatt
     1741 tgaaagcggg cagaaagcgg cagggtgcct ccaccctcac catgcagctt gcgcgcagcc
     1801 tctggctgga caccgataag aagtggaggc gcaagctgtc cgagttgatg atcaccctga
     1861 tgttggagga caaacttacc aagcagcaga tattcgaata ctatgcaaac cagatttatc
     1921 tcggcaggcg gggcacatac agcatccacg gcttcggcga ggcggcctac gcgttcttcg
     1981 gaaaggatct caaagacgtg aatctgccgg aggccgcgct gctcgccggt gttgcgcagc
     2041 gccccggcta ctccgatccg cggcggtatg cggaacgcgc ccgccttcgg cgaaacgttg
     2101 tgctgtcgct catgaaggag aacggccaca tcggcgaaga gcaataccgg cgagccgccg
     2161 ctgctcctgt agagattgcg aatgcgcccg cacaggcgca ggaagcgccg tatttcctcg
     2221 atcaggtcaa caacgaactg agtgagcgct tcgcctcgga ggacaccggc cagttgaata
     2281 tctacacgac gctggacatg cgcctgcaac gcgcggccac agaggcggta gacgccggga
     2341 tgaagcatgt cgataaggag ttgaagaggc aaaagatcga tggcgtggcc caggttgcgt
     2401 tgatcgcgct ggatccgcac accggggaag tgaaagcgat tgtaggcggg cgcgactact
     2461 cccaatcaca actcaaccgt gccctcgctc gcaggcagcc gggttctgtc ttcaaacctt
     2521 ttgtgtatgc ggcggcgctc agtaccacca gccgtgggag cggcgaaatc tttacgcccg
     2581 ccaccgcgtt ggtggatgag ccgactacgt tcaacttcgg tcgcaccgcc tacgagcctg
     2641 gcaactacgg aggaaatttc aacggtacgg tcacgcttcg ctctgcgctg gttcgttctt
     2701 tgaacgtccc gacggtcaag ctggccgaga tggtggggta caaaaatgtc gtcgcgctgg
     2761 cgcggaatgc ggggttgaac gatgaaattc gggcgactcc atcgatggcg ctcggctcct
     2821 acgatgccac gccgctcgaa atcgccggcg cttacaccgt gtttgccaac agcggcacct
     2881 tcgtgcgccc cactctgatc cgggaggcgc ggacacccgc cggtaaggtt ctttattcgc
     2941 attcgccgaa gcgcagcccg gttctgaatc cgcagatcaa ttacctgatg gtcacgatgc
     3001 tgggcgatgt catgaattgg ggcaccggat cgagggtggg ggcactgggt ttcacgcagc
     3061 cgtcctacgg caagacaggc acttcacacg acggctggtt cgcggggttt accaacagtc
     3121 tgctctgcgt tgtgtgggtg ggcttcgacg actaccgcga gttgaagctc gaaggatcgc
     3181 ggtcggcgct gcccgtctgg gtggagttca tgaaacgcgc gtccaaaatt gccggtcccg
     3241 cgagaagcat cgaggctccg gccggtttga cgagcgaaat tgtgtgtgcg gactccggca
     3301 aactccccgg gccgttctgc cctaaagtac gcgaggagat tttccttccc ggtacggagc
     3361 cggtcgacta ttgcgacctg gatgccggac ctcaatggcc cggggcggag ggcgccggga
     3421 ctcactccat cgcttccggc cctccatccg ccgcaaccgc gctccccgct tcctacgagc
     3481 ggtaacgctt aacactttcc gttcaggaac tgtttgatgg accggatagc ctgccggctg
     3541 cgatgatggt tttcaatcag ggcgaaccgc acgaagcctt caccaagcgg gccgaagccg
     3601 ataccgggcg atacggcaac cagggctttc tccagcaact gctttgaaaa ctccagtgag
     3661 cccaggtgcc ggaatttttc cggaatcggt gcccagacaa acatggagcc gcgcggaggc
     3721 gacacgggcc agccggcgcg gcacagcccc tctatcaaaa ggtcgcgccg gttctggtac
     3781 aacgtacgga tctgatgggt atcctcttcg cattcccgca gggcgatgat ggcagcgatc
     3841 tggatgggtt ggaaaatccc gtagtccagg tagctcttga tgcgggccag cgcggcgatc
     3901 attctccggt tgccgagaca gaaaccaacc cgccaacctg ccatgttgta gctcttggaa
     3961 agcgaaaaga tctcgaccgc gtgttctttc gccccttcca cctgaaggat gctgggtggt
     4021 tgatagccgt caaaaccaag atccgcgtag gcgaaatcgt ggacgaccat tgtcccgtga
     4081 tgagctgcaa gccggatgat ttcgcggtga aaatcgaggt cgacacactg cgtcgtcggg
     4141 ttgtgcggga aggagatcag aatcagtttc ggtttttttg acaggctcga gtaaagctgc
     4201 tccaggccgt tcaggaatgc tcccggattc ggcatcggga ccatgcaggg catgccatcg
     4261 gccatggcta cgccatactg gtgaatcgga tatgccggat tcggcgacac gaccggatcg
     4321 cccggcccga tcacggcgaa cagaagatgc gccagggcat ctttagcgcc gatggttgca
     4381 atcgcctcga cgtcgggatc cagatccacc ccataattcc tcttgtaccg ctttacaatc
     4441 tcctgcctca gcttgggaat gcccttcgaa agcgagtagc ggtggttgcg cgaattccgt
     4501 gcggcttcaa ccagcttgtt gacgacggcg cgcggtgtag gcccatcggg gttgcccata
     4561 ccgaaatcca ccacgtcttt ctgctcggag cgcagacgcg ctttcatctc gtttacaacg
     4621 gcgaacacat aggggggcag tttctcaatt ctgtaaaatt cccctgggtc cgaaaaagac
     4681 atagctacta ttgtaaatgg agactataca acgtgagcgt actcagcccc ggagccctct
     4741 tgaacgcggc gatcttcgcc atagtgggac tcgttctgtt tgcggtggtc ctgtccatga
     4801 tcgagaagtt cacgcccaaa ctcatctgga ccgaggtcat cgaaaagcag aacctggcgg
     4861 cggcggtgct ggcaggcgcc atcgcgatcg cgctggctct cattgtttcc tccgccgtgc
     4921 attaaccaaa aatcgcttcg aaggcatcat cgatgaacgg gtactggaac cggaatcccg
     4981 cggccaccgc cgcgtgcggc agcactcgct ggctggaagt caggacttcg gccatctcgc
     5041 cgtacaacag tttcagtgct aagaccggca ccggcaggaa cgcgggccgg tgcagagcct
     5101 ttgccagtgc gcgggtgaaa tcgatgttcc ggaccggatt cggcgtggtg ccatttacgg
     5161 gaccggaaag ccgcggctct tccactgcaa accggatcag ctccaccagg tcatcggcgt
     5221 ggatccacga catccactgt tgcccatcgc caagtttgcc tcccacaccg aggcggaagg
     5281 gtgtgagcat ctgccggagc gcaccgcctt cgcgccccag cacgatgccg ttacggatcg
     5341 tcaccacccg gatacccgcc gcgcgcgcct gttcggccgc ccgctcccac tccgcgcaga
     5401 tatccgcgag aaagccggtt cccggcggcg aggcttctgt cagcagatcg tccccgcgcg
     5461 aaccgtaata gccgatggcg gaagccgaca cgagcgttcg tacctgtttc ccggtcacct
     5521 cgatccggct caccaggttg cgggtaccct cgctgcggct ttcgcgaatc cgccgtttca
     5581 cctccggggt ccatcgctgt gcgatcggct ccccggcgag atgcacaacg gcttcggctt
     5641 tttcgaaggc ttctgcggga cgtgtcccgt ccgtcacgtc ccaagcggag aacgcgaccc
     5701 gctttgcctg tccttttggg ggttttctcg ccaggccgtg aaccgagtgg cccagggtgg
     5761 tgaggcgctc cagcaggcgg gtcccgagaa agccgctcgc gcccgtcacg gtaatgttca
     5821 tagggccgtc ccgctacaat aaagctatac catgcgagcc tggaaccggg cgaaactgct
     5881 ctgggggtac ctgcgacgaa aatcggagct tggtggattg ccggtggaat tcatcgtcga
     5941 aaccaccgcg aagtgcaatc tatactgtcc gatgtgtccc cgcgagaccc acaagcaacc
     6001 caaggcggac atggcggacg agatctttga gaggctggtt cacgagtcgg gcaaaactag
     6061 cgaacacatg atgctgatcg gactcgggga gcctttcatg gatcgccgga tcttcgatcg
     6121 tatcgagtac tgcgaacggc atgggatctc gacgctgctt tccaccaacg gcaccttcct
     6181 cgacgagaaa acatcggagc gccttctcgg cagcactctc gagcacatca ctctgagttt
     6241 cgacggagcc accaaagagt cgtacgaata ctatcgcaag ggggcaaagt tcgaaaaagt
     6301 gcgcgacaac ttcgtgcgct ttgcccgcat gaagcacgaa cgcaagtcga aaatgcagat
     6361 cgtcgtgcag atggtgcgca tggagcgcaa ctgggacgaa gtggaagagt tcacccgttt
     6421 ctggcgcgct gtcccgggtg tcgaccaggt acgcatcaaa gcggacgaaa cgaatgtgtt
     6481 agaccccgct tcctcccacg cggccgagga ttggaagtat ccctgccact atctttggcg
     6541 cgggcccctg tatgtcaagc agaatggcga cgtctacccg tgctgccaga gctatatgct
     6601 ggatggcgct ccggtgggaa atatcgaaac ggaatcgctc ggaagcattt ggaattccgg
     6661 cgaaatgcag cggatgagac ggctccacgt cgaagggcgt gccgggaaga tcgacatctg
     6721 ttcgcgatgc tgcacgacga ttccgcaccc ggcgctggtg gcgggcagcc tgctgctcca
     6781 cggccgcacc gttcgcaggc tgttgccgct cgtcgagcgg ctggcgtatt tttcgaagat
     6841 gccggcgcgg ctgttacggc cgccgaagcc tcctaatcgc cggccggaac tggtgcagat
     6901 atccgggccg ccggggaagg gctcgccagc ggattcctga gattgtgctc cgcgtaatag
     6961 cccggcctca cccgctccac gatctgggcc acgggtcttt ccagcacttc gaagaacggc
     7021 cgcgggtata agccgatcca gaacgcccac accagcagcg gcgcgaagat cgccatttcg
     7081 cgaagcgaca ggtccttcag cctcgcgttc ttctcggaaa cttccccgag caccgttcgc
     7141 tggtacagcc agatcaggta cgcggcgccg agcagaattc ccaccacgca ccagaacgcc
     7201 cagttgaacg acatctgaaa gaccccttgc aggatcgtga tctcgccgat aaacccgttc
     7261 agcggcggca ttcccatgga gctgagcgcg gtgatgagga acacgatcgc gaacacgggc
     7321 atgatgcgca aaagcccgcc atattctgaa atctcgcgcg tgtgccggcg ttcgtaaacc
     7381 acccccacga tcaggaacaa cataccggtg gagatgccgt ggttgatctg ttggagaatc
     7441 gatccggcaa tccccgccgg gttcaaggcg aagattccga gcgtgcaaaa gccaagatgg
     7501 ctgacggaag aataggccac cagctttttc cagtccttct gcatcaggct caccagcgcc
     7561 ccgtacacaa tggcgataat cgacagcacc gccatgatgt tcacgatggt cggattcttg
     7621 gccgcgtccg gaagcagcgg aagcgaaaac cgcaggaatc cgtaagtgcc catcttcagt
     7681 agaacgctgg caagaatcac cgagcccgcc gtcggcgcct ccacgtgggc gtcgggaagc
     7741 cacgtgtgga acggccacat cggcactttc acggcgaagc ccaggaagaa cgcccagaac
     7801 acccaccact gcaccgtccc ggaaaggtcg gtttgcagaa ggtccgcgat gttgaacgtc
     7861 agcacctgaa actgcgagta gtgctcgaag tacagcatca ggattccgag cagcatcagc
     7921 acggacccga tcagcgtata aatgatgaac ttgatggcgg cataaacccg gcgcggcccg
     7981 ccccagatcc cgatgatgaa atacatcggc accaggacca gttcccagaa cacgaagaac
     8041 aacaggaaat cgagcgccat gaaaacgccg agcatgccga cctgcaacag aagaaagtaa
     8101 gcgtaatatt ccttaagccg cgtctggatg gcattccagc tcgacaggat cgcgagaaac
     8161 cccagcaagc ccgtcagcac caccagcagc aggctgaaac catcgatgcc caggtggtac
     8221 gaagcgccca gcgagggaat ccagtccgcc ttctcgacga actgaaaatc tttcgtgcga
     8281 tcgaacccga aaatgagagg caggcacacc aggaaaccgg cgaaagaaac gatattcgcc
     8341 cagagtttca ccgctttaga gttcgacgaa ggaatgaata gcaggacaag caggccgatc
     8401 aggggcgtga agagcacgat cgacagaatg tggtccataa gaatcggctt tcttgatatt
     8461 gaagtcttat attatcccat tcgcgtcacg cggtgctccg gctacgccaa cgcttccacc
     8521 gcgtgcgtcg ccacgcggcg cacgtaatcg ttttcgtctt tcaacgcgtc ctggagagac
     8581 tggacggcag agcgcgcaac gctacccatc tcgtccagta cgatgacagc atacaggcgc
     8641 acccactgct cgctgccttt caattccgcg gtaagtacgg gcaaggccgc ttcggctttt
     8701 ccggctttcc ataacgcacg tgccgcggca acgcgtacca tgccgctcga atcctgaagc
     8761 gccttccgca aagcttcctc tgccgtcgcc gcattctcac ccaggttgcc gattcccgtg
     8821 gcggcccaat accgcacacc gggttcggca tcgccgagag cggccaccag tttggcgagc
     8881 gccggtttgc cctgttcccc cagctctacc atctggcgga gccgccgcgg aaggtccttg
     8941 ttttcgggcc tccgcaggat ggcatagcgc gaaccgagct cccggtcggc cttctccagt
     9001 tcgggttccg gcaccagcgc gaggtccctg gcctcttcca tccacgtcag atgtgctttg
     9061 cggagcctct ccagcacagc gcgatgctca acggactccg caaggttctt cgtctcatgt
     9121 ggatcggact cggcttcata gagctcctcc accggcttgc ggtcggccat gaagtgcgcc
     9181 gcggccgggg gcaatgtgcc ttcggctttc acacgccgca attcctgcat cacaggactt
     9241 tgctcacagt agctgaggaa ctgcgagtag ggcttgtaag cctcgtaatt gcggatgtac
     9301 cggtatcgct tgtcgcggac ggagcggatg atgtcgtagc gttcgtccat gcggtcgcgc
     9361 atgccgaaaa cgtattggcg cgggggttgc agtttcggac cgagaaacgc gcgcccctgc
     9421 atatggcccg gaatcggaac tcccgcgagg tttaacaccg tgggcgcgag atcgacaaag
     9481 ctgatcagct cctcgctcac actgcccggg gtgccctgtc tgtcccgccg gaatttttcg
     9541 gggatgtgaa cgatgagcgg cacatgcgtg ctcgattcgt aaagccagcg cttggcgcga
     9601 ggcagcccgg agccatgatc ggaccagtag aacacgatcg tgtcatcggc catgccgtcg
     9661 tcttcgagtt gccgcaacag atcgccggcc caatagtcca tcgcggtatt cagctcataa
     9721 tggttcgccc actggcggcg caccgttggc gtgtccggat aatatggcgg aagcgtcagc
     9781 ttggccgggt cctgccggtc gcgcggcgcc aggcgcgcgg tctgcttcgc aaacgcttcc
     9841 gccggggcgc gcacctggct ttcgtgcgtc gctacgttat tgaagacggc gaagaagggc
     9901 tgtcccgccc gccggttgtg ataatgcgct ttgccgctcg attcgtccca ggctccctcg
     9961 ggcacgggaa aattgtagtc cgtcttctgg ttattcgtgc aatagtagtc ggccctgcgc
    10021 aggtattcgg ggaagcattt cacgaagtcc ggcagcgggg cgctggagcg catgttctgc
    10081 gagccgagcg tgctttggta gacgcccgtg ataacacagg aacggctagg cgcgcacact
    10141 cccgcggtag tgtaggcgtg agcgtagcgc acgctgcgct tcgccagcgc gtccagattc
    10201 ggcgtgatcg cgtgggcatc cccatagcag ccgagcaacg gcgacatgtc ttcattcgac
    10261 agccacagga tattcggccg cggtgcgccg gtttgggcgc cgcgaagact cgaagcggcc
    10321 agagtgtgcc cgatgaattc acgtctcgag actggcatgc acgttacctg ctgacatcag
    10381 aactgcagtg taagttgccg gcggccgaga tacagttgcc gcgaagagcg cggccgggcc
    10441 ggaatgtgca gcaatacccg cccttccgtt tgcgccgcga cgtactcggc gaccgctttc
    10501 tccgggtcgc catcggagag cgcaggcccg cccgtctcat ggatccacag ttcggtgacc
    10561 gagtttagca gggcctccgt gcgcccatcg gcgcacaccc actcgccgcg ccgcagaata
    10621 gctttccggt tcctgactct gataagaacg ggcaattgcc gatcataaca aaggcgcgcg
    10681 tggaaacgct ctttctgccg cttcagaaac gcaattcgac gccgccggaa gcaaccaggc
    10741 ccgggtcgcg ctgggttccc gccgtgcggc tggcggatcc atccggcccg atggtggtgc
    10801 gatcgaagcc caggtcgcgg cgatggaaca ggttttcaat tcggctgaac acgccccagc
    10861 gggacgatgc aacccaggcc agcgaggcat tgatcttgct gaagctgccg tagcgggtga
    10921 ccatcacttc gtcattcagc atttcctggc tgacgccgcc gtcgtcgtag tagccataga
    10981 tggacaggat cagccgcgga tgcaaatcga cgtgcgaccc gaatcccgcg ctatagttcg
    11041 gaagcggggc aaagcgcagg tccacgtccg ccgcctccgg gtttgtgctt gcgtcacggt
    11101 agctccggag ccatgtgaag gaagcgaacg ggcgaagccg ccagatgcgc cactccgtcc
    11161 aaagctccgc cccgcgcgtg cgggcgtcgt cgtagtaggg caggcccgaa tggctgtcgc
    11221 cgaaattgaa caggtccgat tcacgcgcgg aaaagaccga cacgcggacc ctgccggttc
    11281 ggttgcctcc gaacgatatc ggctgttcga gtccaatctc ggtggtttcc gtggtcgagg
    11341 gatccaggcc cgttgccgga ttccgccgta taaactcccc gccgccccac cacagataaa
    11401 acgctttgtt cagtttgtat cccgtggacc acgacccgaa gagccgcgtc tgggtcaccg
    11461 gcacaatcca ggcggcgccc gcatcgtaaa gcacgcgcgc atcgttgcgg aagccgttgt
    11521 cgacgttggc cactcgcacg cctccgttca gcgtgacggc acggcttatt gcaagggaat
    11581 tgcgccagta gcccgcctgt tcgcggaaag agaagctgat gggcgaggtg gtgatatccc
    11641 ggaaccggtc ccaggtgaaa tcgccgcctc ccgatgccgt ataggaaacg gagtccgtaa
    11701 tgggagtgcg ccagtgaaag tgctgaacgc gcgggtcgct cgctgaggcc ggttgcggcg
    11761 ggagagcccg accggaacgc cggccgcaaa gtcctcgatg cggtcttcgt gaaaccagtt
    11821 ctgcgtaacg ccgagaccga agccgaggtc ctccgatctc cgccagtcgt agcgcgtcgc
    11881 caggaaatgg ttgctcttgt ccgtgtgaaa ttcgcggcgc gcgggctgcc ccgctatggt
    11941 cgcgttctga aaatagtcgt ggcgctctcc gcctcccgcg tacattgttt cccaccgtga
    12001 ggaccgcgtc acgcgcaaac caacctttgc tactcctgaa ccaagccgca gtccttcaat
    12061 gggccggggg aaagtatcat acgctgtgcc ataatccgcc agcgagcttt gccccgcgac
    12121 gtaataatcc acgcgcccga atcctccgcc ggtgtccccc atcacgatgc cttcccccag
    12181 gctgagcatt tccagccgca ccgacgaagt gatgtttccc accgagccgc ggcggatctg
    12241 attgcggacc acgccgcccg aggcctgccc gtacgacacg ccaagcccgc ccgtcgccac
    12301 actgagcgat tcgacagcga tggagggaat gaccgaagag aaccccgcgg tatagccgcc
    12361 gtcctccgca ttgaccggag tgatgttgat ggccgggtag tcgtcaatcg tctcgacaat
    12421 gccccaagcg tggccgcccc ggatgtgtgt cggcaggctg aagcggtcgc ggcccccgta
    12481 ggccgcagtc atcacgccgg gcagcaggcg cagagcggaa tagttggcta ccgggttgaa
    12541 gttcaccagg tcgagtccgt tcagggtgga gatggatttg ttgcggttgt agatctcatc
    12601 cacttgcatg cgcaggtcgg cggggtggga atcggtcacg gtgactcccg tccgaagtgg
    12661 agcgatgcgc agatgcagcc gcagcgttgc ggctgcgtcc ttgccggcga tcgcctgccg
    12721 taacacttca aatccctttg cggtgacgcg cagcgtcgcc ggcccgggct tcacgtcctc
    12781 gaaccggaat cgcccttctg gatcggcgtg ccgagtctcg actgtaccgt ccggaaattc
    12841 cagggcgact tcggcgccgg acaccggctt ttcctgcgaa tcgacaactg tgccctcgac
    12901 ggcagtgcgc tcctctgcga aggccggcat agcaccggac agcaggacag ccaggcaaac
    12961 ggccctcagc aggcaccatc ttgaaatcag cgcgtatcgg gtttcagcca cacattacct
    13021 ctttcgttga aagctatggc tggcgaccct aaaatgaaag aggtcgccgg ccttccggtc
    13081 gttgatccgg gggcgccgcg gggcttcgcc taaaagactg ccgtggcgcc cccgtccagg
    13141 tttttagttg taacccagta tcaatacctc tttatgtagt aacgctgttg caagtcgttg
    13201 tcaactggat tctgtcaacg gctgtcacgc ccgcttcgaa tgcgcgccgc tgaaactaca
    13261 atccctgctc cagcggcagc acaatcgtga atcgtgatcc ctgccccggc cggctcacaa
    13321 cttcaatgcg cccgtggtgt tccttcacaa tctggtagct gatcggcagc cccagtcctg
    13381 tgcccacctt gacgccgccc tttgtcgtga agccgggatc gaagatgcgc tccatgtcct
    13441 tctcggaaat tcccacgccg ttgtcggaga tttcgatctt caaccaatcg tcctgctgcc
    13501 acgtgcgcag gcgaaccact ccctgcgccc tcccgctctg ccggatggcc tcgtttgcat
    13561 tcagcagcag gttcatgaac aactggttca gttggcgcgg cgaacactcg atctccggca
    13621 cgtccccgaa ctctttcacg acttccactc tgccgttgaa ctcgtgattc agcagcgcca
    13681 gcgtgctttc caggctgtcg tggacgtggg cgcgctgcaa atccgcccgg tccagttgtg
    13741 cgaactgccg tagattcgtc acaatgccgg tgatccggtc gcaggcgttc ttcgattgcg
    13801 cggcggtggt gctcagaatg tcgataaagg gctgaacctg cccggcgatt tcgggctcct
    13861 ggcttcgagc ccaatcgagc agccgctgcg acgcgcgttc gatcgtatcc gtgctgcttc
    13921 tcagtgcgcc cagtggtgtg ttcaactcgt gcgccacgcc ggccaccagc ttgcccatcg
    13981 atccgagctt ctccgcctgc accagcgccg cttgcgaatt cttcaaccgc atcagcgcgt
    14041 gttgcagttc ctcttcgtac gactcgtttt cgcgaagcag ccgccgctgc tccaccgcgt
    14101 tatgcgtcac ggattgcagt tcggccgcgt cgaagggcca caggatgaag ttatgcgctc
    14161 cctcgcgaag cgcctggacc gccgtgcgct ccaacccctt ggcggtaatc agcaccacca
    14221 gcggaggccg cggttcgctc gccaggtgcc gcatcagccc cagcccgtcc atcccctcca
    14281 gcccggcgtc ggccagaacc acatcgggat ggaactccgc cagtttctct atggtttccg
    14341 cggcggtgcc ggcttcttca agctcgtacc ggccgcccgc cagcgcatcc cgtatctgat
    14401 gaacggcatt tgaatcgccg tggctgatga ggattttttg caggtctttc aaacgtgagt
    14461 tctttcccgg gtttcatttt acgagatccg gatgcttcgg ggactcgctt cgccgggcaa
    14521 taccaccgac accgttgtgc cctctccggg agtgctcaca atgctgattt ctccgccgtg
    14581 ctcacgcaca atttgtctgg cgctgaacat accccaattg gctgaggaaa tacgtcccgc
    14641 ggtctcccgg aatcccggct cgaaaatggc cgccatgtcc tcaggcatca cgccgcgccc
    14701 attgtctccg atctgcactt cgatctcggc cccgcggggg agcgtgccga tcgcgatacg
    14761 cccgttgccg tttaccgctt ccatcgcatt gttcagcagg gtggcgaatg ccgcgctgag
    14821 ctgctgcggc cggcagatca ctcgtgggac cggcttgagg tccagtacga tctggtggcg
    14881 gctgtccatt cccgggcgca ccagcgccgc tacatcctcc agcagatggt tgatgtcgat
    14941 ccttcgtatc tccgcctggt caagattcgt gaatcgttgc atgcgtaaga tggtcttccg
    15001 gattcgctcg ctcgattcgc cgatcgtctt ccggagctcc gcctgcagcg acacgtagcg
    15061 ctgctgttcc accggtgtgg cgctgacctg tttggaggag agtaaaagga gcgtatcgat
    15121 agcgctcgtc aatgcgccca acggggagtt gaattcgtgc gacagtccgg ccgcgatcct
    15181 tcccagcgtt gcgctgtgtt cggacgaaag catcctctgc tgcgcgaccc gcaggttgtc
    15241 gcaggcagtc agcgtctcct ggtatgcctg gtagttccgg tgccgctgtt catatgcgac
    15301 tgccgtcagg ctcacggcca gcaccgacag aatcaaaaag aaaaacaggt aagtagggtc
    15361 gagcgtcgtg gcgggaggca gcgaaaaact ctccggccgc gatgccgccc atgcatagaa
    15421 gcccgccagc gacaggcaga gcgccagcgt atgcttgggc ttcagtggaa tcgccgcaat
    15481 ggccaccaga accaccagcg tgacctggct cggtatgaaa ttgtccatgt acggcgcccg
    15541 cggtgcgatc caaagagaga accacgtcgc cacgctgccc gaagcaaatg ccgtcagcac
    15601 cgcaatcgtc cgcgaccggg cataggccgc cggaagatgg tgcatcgcca gcaggaagcc
    15661 tcccagtgcg atcataatgc cgctttgcag cagccgtact cccaccattc cctggccccg
    15721 cccgactgcg aaatgcgcta gcgtaatggc cacggccgca ccggcttgaa cggccgcgat
    15781 gatcttcaaa cactggtggc tgaggcgcag gatctcctgg cggaatcctt catcctgttc
    15841 cgcgtcgttg gggagaaggc atttccggag cgcctgtagg ttcatcaaag ggcttcttga
    15901 gattactgta ccgcgtcctt cccctcttta acgccggaaa ggctgtactc gatccacaag
    15961 cgcctgaaat cgcggctccg gtcgcaacgg ttccattcgc tggtccacca ggatggatgg
    16021 cagcggccct tcctgattgc cggtggatct ttcgagcaga tcgagagctc cgggtatgtc
    16081 tccgcggccg gccctcacga tagcggcgtg acacaggacg ggccgtccgc gatcgtgaag
    16141 gtcgagcagt tgtttctcaa tctccagcg
//
LOCUS       DASTMK010000555         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_444441, whole genome shotgun sequence.
ACCESSION   DASTMK010000555 DASTMK010000000
VERSION     DASTMK010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444441"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2136)
                     /locus_tag="VFQ79_22900"
     CDS             complement(<1..2136)
                     /locus_tag="VFQ79_22900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142589.1"
                     /translation="MSRLRAYLKCICAASLLGPAVLSAQLTVSIIRGTATDPSGAAVA
                     NAVITIVNTDTGLKRESITNTAGDYELPDLPRGRYKLTATAAGFRGFTANDVVLESTQ
                     VRRINVPFEIGAVGAEITVTAGAAVISTDSSKIQGAFERRTFEDAPLIGDGRNPGLLL
                     STLPHIQQSGSIYGVQFAGQPGSQIQQGIDGMTTDGAINQVSNIHDMEELVAVPVNNT
                     AEYSRVGFYNMVTKSGSNEFHGQAHYWLRNSALDARDFFQSQKPVAKSHTMHGELTGP
                     IFRNRTFFYGAYSGQRWPGGSFYLRDVPTAKMRQGDFSQLLNQTRPVVVRDPLNGQPF
                     AGNIIPAQRINPVATKVMQNYLPAPNLGGPDELARNYGYLWPWPEDLRIGNYYSFRID
                     HHLTANNRLYGRAITNWIDYALSRELPTLGWTRNRRNQHIVIEDTHVFSPALVNTFRF
                     GVYHVVVDDGKTVDGFTPQVGDQVVQELGIQGVNSQNLSAMGFPRMDIAGYPSLRNQP
                     GGLVLDDKQWTFTDSLTWSKGSHVLKFGGEIKPFSRFEGLVPEGNYGNFVFNGSLSGY
                     SYSDFLLGLPFSSQRLDPLTDRTRLDSEIGFYAMDTWKVNSRLTLDLGLRWERFGAAT
                     YEDGLIYNWDPETGNVIVPRGRLDQISPLYPTNTISVVEGDVKQRPSLSNWAPRLGAA
                     WRPFGEKTVLRGSYGLFIETLGLFARAQSG"
     gene            2303..2788
                     /locus_tag="VFQ79_22905"
     CDS             2303..2788
                     /locus_tag="VFQ79_22905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142590.1"
                     /translation="MNAQEVFCQNIRNTIFLILIVFFTSIAGQAADPDFSGTWRSNPS
                     QSTVQRAPLPPGKLLNIQHRGITVTCEALTEASTVENRSFTTDRKESRHESDGATRST
                     VAKWEGDNILVNTIVLMRSGAQYIQVGALDTLPRSKYAPNPAADHQFARQLRVPDHST
                     V"
     gene            complement(3083..>3796)
                     /locus_tag="VFQ79_22910"
     CDS             complement(3083..>3796)
                     /locus_tag="VFQ79_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0142591.1"
                     /translation="GKHVKVNDTWLRVVGVLAEQVADSGSGRAVDRNNVIFIPISTFR
                     YRFWDASSYLKDELDGVDLRLKAGEDSVAAAKIVTAILASTHRDAEDFAVTIPAALLA
                     QQKRTQTIFTYVMVAIAAISLLVGGIGIMNIVLATVMERTREIGIRRAIGARRGDIMR
                     QFLTESVVISVAGGLLGVGFGIFLSWSIAQVAEWKTIVTTTSILVAFCVSALVGVLFG
                     IYPAMKAARIDPIEALRYE"
BASE COUNT          787 a         1137 c         1093 g          779 t
ORIGIN      
        1 gccgctttgc gcacgcgcaa aaaggcccag ggtttcgatg aataaaccgt agcttcctcg
       61 aagaacggtt ttctcgccga acggacgcca cgccgcaccc aggcggggcg cccaattgga
      121 aagggaaggg cgctgcttca catcgccttc gacgaccgag atggtgttcg tcggatatag
      181 cggactgatc tggtccagtc tgccgcgggg cacaattacg tttcctgttt cgggatccca
      241 gttgtagatc agtccgtctt catacgtggc cgctccgaaa cgctcccacc tgagccctag
      301 atcaagcgtc aggcgcgagt tgactttcca cgtgtccatc gcatagaagc cgatctcact
      361 atccaggcgc gtgcggtccg tgagcggatc cagcctctgg ctcgaaaagg gcagccccag
      421 cagaaaatcg gaataactgt agccgctcag tgagccgttg aatacaaagt tgccgtagtt
      481 gccctccggc accagccctt caaaccggct gaacggtttg atctcgccgc cgaatttcag
      541 tacgtggctt cccttcgacc atgtcagcga gtccgtaaag gtccattgct tgtcatcgag
      601 caccagcccg ccgggttgat tgcgcaggct cgggtacccc gcgatgtcca tccgtggaaa
      661 gcccatcgcg ctcaggtttt gcgaattgac cccttgaatc cccaactcct ggacaacctg
      721 gtcacccacc tgcggcgtga acccgtccac tgttttcccg tcgtccacca caacgtgata
      781 cacgccgaag cggaaagtat tcaccaacgc cggcgaaaac acgtgcgtgt cctcaatcac
      841 gatgtgctga ttgcgccggt tgcgcgtcca gccgagcgtc ggcagttcac gcgagagggc
      901 gtaatcgatc cagttcgtga tggctcgccc gtacaagcgg ttgttggctg tcaggtgatg
      961 gtcaattcga aacgagtaat agttgccgat gcgcaggtct tcgggccagg gccacagata
     1021 accgtaattg cgcgccagct cgtcgggtcc gccaaggttc ggggcgggaa gatagttctg
     1081 catcactttc gtcgcgaccg gattgatccg ttgcgccgga ataatatttc ccgcaaacgg
     1141 ctggccgttg agcgggtctc gcacaacaac gggccttgtc tgattcagca gttgggagaa
     1201 atcgccctgc cgcatctttg ccgtggggac atccctcaaa taaaaactgc cgcccggcca
     1261 acgctgccca ctatacgccc cgtagaagaa cgtccggttc cggaaaatgg gcccggtgag
     1321 ttcgccgtgc atcgtatggg atttcgcaac cggcttctgg ctctggaaaa agtcgcgggc
     1381 gtccagcgcc gagttccgca accagtagtg cgcctggccg tggaattcgt tgctgcccga
     1441 cttagtgacc atgttgtaga agcccacccg ggaatactcc gccgtattgt tcaccggcac
     1501 ggccaccagt tcttccatgt cgtggatgtt cgagacctga ttgatggcgc catccgtggt
     1561 catgccgtcg attccctgct ggatttgaga gcccggctgc ccggcaaact ggacgccata
     1621 gatactgccg gattgctgga tatgcggtag cgtcgatagc agcagccccg ggtttcgtcc
     1681 gtcgccgatc agcggggcat cttcgaacgt acggcgttca aacgcgccct gaattttgct
     1741 gctgtcggtg gatatgacgg ccgctcccgc cgtcaccgtg atctccgcgc caacggctcc
     1801 gatctcaaac ggcacgttga tgcggcgcac ctgtgtactc tcgagaacga cgtcattcgc
     1861 agtgaagcct ctgaaaccgg ccgccgtggc cgtcagtttg tatcgcccac gtgggagatc
     1921 cggcaattcg taatcacccg cggtgttggt tatggattcc cgcttcaatc cggtatcggt
     1981 gttcacaatc gtaatgaccg cattggcaac cgctgcgccg gaaggatccg tggcggttcc
     2041 ccgaataatg gaaacggtca actgcgccga tagcactgcg ggtcccagta gagatgccgc
     2101 acagatacac ttcagataag ctctcagtct cgacatttcg agttcctctc cagagtgacg
     2161 aatggactac tgggcgatat tgtatgtcag acagggttct tgccgaaatc gccttcgata
     2221 tacccgtgga caagctcagc tcgtccggct gaacaaccgg tggccattgt gttccggcgc
     2281 ggcacccgtt aggatgtggt tcatgaatgc gcaggaggtt ttttgccaga acatacgaaa
     2341 tactattttt ctgatactga tcgtgttttt cacttcgatc gcggggcagg ccgccgaccc
     2401 ggacttcagc gggacgtggc ggtccaaccc ctcccagagc acggttcaaa gagcgccgtt
     2461 gccgccaggc aagcttttaa acattcagca tcggggaatc accgttacct gtgaagcgct
     2521 cacggaggca agtaccgttg agaaccgctc ctttactacg gaccggaaag aaagccggca
     2581 cgagtcggat ggcgcaacac gaagcaccgt ggcgaagtgg gaaggcgata acattctcgt
     2641 caataccatc gtcctgatga ggagtggtgc ccaatatatt caggttggag cgttggacac
     2701 tctcccgcga tcgaaatacg ctccgaatcc agcggcagac caccagttcg cgcggcagct
     2761 cagagtcccg gatcattcta cagtatgaac gctccggcgc accggctgcg gtcccgcaag
     2821 ccgccgccgc tagcgacttg cggtacgcga attcattccg gccgccagcg gcaccgatac
     2881 ccggacgaat cgaccgcttt acgatcgccc ctctgtcggc gagcaggcca agcaccatat
     2941 tgagacgcag gagcgggagg agacattcgc ctccctgctt ttgacgttcg cctacaacgg
     3001 ggaccgcgcg agtatcgccg ccgggggaga ccggttttga agttttccta cgcgtgggag
     3061 gcgaagagtt ccggtttccc ttctactcgt agcgcagggc ttcgatggga tcgatccgcg
     3121 cagctttcat agcgggatag ataccgaata gaactccaac caaagccgag acgcagaatg
     3181 cgacgaggat cgacgtcgtc gtcacaatgg tcttccattc ggcgacctgc gcaatggacc
     3241 acgaaagaaa gatgccgaac cctacgccga gcagtccgcc ggcgacagag atcaccacgg
     3301 attcggtaag gaactggcgc atgatatcac cgcgccgcgc accaatagcg cgccggatgc
     3361 cgatttcgcg cgtacgttcc atcaccgtcg ccagcacaat gttcataatg ccgataccgc
     3421 cgaccaggag cgagatggcg gcgatggcga ccatcacgta agtgaagatc gtctgcgtac
     3481 gcttttgctg cgccagcaac gcggcgggaa tggtaacggc gaaatcttcc gcgtcgcggt
     3541 gagtggaggc caggatcgct gttacaatct tggcggcggc gacactgtcc tcccccgctt
     3601 tgagacgcag gtccacgccg tcgagctcgt ctttgagata gcttgaagcg tcccagaagc
     3661 gatagcggaa ggtgctgatc gggataaaga ttacgttgtt gcgatcgacg gcgcggccgg
     3721 agcccgagtc tgcaacctgc tccgccagga cgcccacgac gcgcagccag gtgtcgttga
     3781 cttttacgtg ttttcc
//
LOCUS       DASTMK010000556        10243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_284857, whole genome shotgun sequence.
ACCESSION   DASTMK010000556 DASTMK010000000
VERSION     DASTMK010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284857"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFQ79_22915"
     CDS             <1..295
                     /locus_tag="VFQ79_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0142592.1"
                     /translation="ATIVHALKPRVAVMNNGAKKGGSPETWDVVRKSPGLEDLWQLHF
                     AIAGGEAHNSPQSFLANLHERCEGNWIRLSAQPDGSFTVFNSRNRYQKTYPAR"
     gene            373..1623
                     /locus_tag="VFQ79_22920"
     CDS             373..1623
                     /locus_tag="VFQ79_22920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142593.1"
                     /translation="MIGLIHQTFLNMWRHKLRSFLTMFGIAWGICSLVLMLALCDGFR
                     QGQRKNMSQIGDNLVFLYSGRTERQAGGERAGRRIWLTEQGVEAVRANCPLVETAAPE
                     IKRYDTPVASSYNSGRFLVLGVSPEYLKLRNLPVETGRPVQARDVEEIRRVCVLGASV
                     RKQLFEERLPLGGEIRINGYPYTVIGAMSEKMQNSSYDGWDNDKILIPITSLRRDCPP
                     ERATYRPDRVDSILYRPLSVKEWRATQVQVRRALAAVYDFDPLDESAVPVNDSVEGAE
                     LFDRVFDSGEMFLAVIALITLSLGGVSVMNTMMMAVAERTGEIGLKKALGATRHRILL
                     DFFLEGVFLALLSGAVGIGAVWTLAGVVNSLPMPAMFSGLLIDMKSALLALAALGTVA
                     ICSAVPPAWRAAGLTPVEALREER"
     gene            1625..2884
                     /locus_tag="VFQ79_22925"
     CDS             1625..2884
                     /locus_tag="VFQ79_22925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142594.1"
                     /translation="MTARIVLKEAFASLRHYRRRTTVTVLSLAWGVASFVMLMSYGDG
                     FDEAIRRAFLAVGQDLIIMSGGQTSKQAGGLRSGRRIRLELDDVKAIRESAPAVLTLS
                     PEIFGRNMNVVRGPREKQYFGRAVWPEYGRIRNVNLVAGRWINAEDNLNRNRVVVLGS
                     TVAKELFSGIPPVNEEVTVNGLRFTVVGVMDSKVQLANYNRRDNMCFFLPYETMSLFA
                     DVRYPHLILWMPVSGMARQTSIDQVRTIMATLHRFQPDDSKAVEILAFNQFLSIIDGM
                     SLAVKLLLGFVGVLTLSIGGVGLANVMLASVIERTREIGVLKALGGRKKTILGQFLAE
                     SLMVISTGGFLGVLIGTAATKAVGAMPLFESIFQDAADKGSLQLHVSMPAILISLGVL
                     LVVGLIAGMIPAIKAARLDPIEALRYE"
     gene            2926..4524
                     /gene="murJ"
                     /locus_tag="VFQ79_22930"
     CDS             2926..4524
                     /gene="murJ"
                     /locus_tag="VFQ79_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU0142595.1"
                     /translation="MNDSSPSGGKPGSEHTTGRFALFVAAGILLSRVVGLVRLRAFAY
                     YFGVQSDAADAFNAGFRIPNLLQNLFGEGVLSASFIPVYASALARGDEKEAGRLAGAI
                     AAILSLITSAVVLAGIFAAPVLVDLIAPGFQGEKRELTVRVVQILFPGAGLLVLSAWC
                     LGILNSHGRFFLSYAAPVIWNAAMIAALVGFGGRVDQPRLAIALAWGSVAGSLLQFAV
                     QLPVVVRLARRLHLSLGRGMASVRTVLRNFVPVFFSRGVVQISAYVDAMIASLLPTGA
                     MTGLANAQLLYTLPVSLFGMSVSAAELPAMSSALGTEEEIAGALRRRLNSGLRRIAFF
                     VVPSAVAFAALGDVISAALLQTGRFTYGDAVYVWGILAGYSVGLLASTLGRLYSSTYY
                     ALRDTRTPLQFAIVRVSMAGVLGIAFATQLPGMMGVEERWGVAGLTLASAIAGWVEFA
                     LLRHRLNRRIGETGLPLAFSIRVWVAGLLAAAAGWGVKLAVGAAGPLVAAALILPAFG
                     IVYFGMTFALGIPEARAWFRRVRR"
     gene            4563..5069
                     /locus_tag="VFQ79_22935"
     CDS             4563..5069
                     /locus_tag="VFQ79_22935"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0142596.1"
                     /translation="MLTTSVLKKGTRLILDVLGPTIEFLTSPEEADACCMLIGTIPPG
                     GVIPLHHHADFEDFYQLSGTVQVISEQNGTLEWLDVTPGDFVRVPGNAKHAFRNLSNE
                     PVVQLIVTTPRLGRFFQELGKPFVPGAPPRKPAPDVLEHFLRVSADYGYWNASPEENA
                     AIGISLLG"
     gene            complement(5091..5426)
                     /locus_tag="VFQ79_22940"
     CDS             complement(5091..5426)
                     /locus_tag="VFQ79_22940"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU0142597.1"
                     /translation="MLWFLAVSGVVVGSLLPSSSPPIEMIDLLHLSDKLLHSAAYAVL
                     SFLPALHENRWMLAGLILGLLSLGVLLEFGQSYFSDRMFDLHDMIANAVGLVAGLVVA
                     LPFRARMAV"
     gene            complement(5484..6842)
                     /locus_tag="VFQ79_22945"
     CDS             complement(5484..6842)
                     /locus_tag="VFQ79_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEU0142598.1"
                     /translation="MSLWPAGMPAILIPTRRCALTLDAQSIRNHESALQRAIAWIAGG
                     LFFLLLPGTFLGAWNLIVITDAQGAAGLDPAWLQAHGHAQIFGWIGSFILGIGFHSLS
                     KMGGPAGFAARRAWASWACWTAGVFLCWSTNLWPWQWRWMLPASALLELSGFLIFFLT
                     VRRHRRPKIRTEDRPQSHVWMIVVFAGTIGFLVSLLANLAFAIQSAIDGSGPAIAHVP
                     NQRLLVLFTWAFPVVNIWGFSARWLPGFLGLRQANGALLLAALAINTAAVFGALAGEW
                     IAAAGLFLLGAVAAAVALAVFAPATGPANLAGVHRSFPTFVRIAYVWLLLSAVISLAA
                     PVWDTSGGIWGASRHALTVGYISTMVFAIGQRVLPGFCGMRNLFSPALMFTALALLNA
                     GCVLRVGSEIGAYEGHLPWLWPLLPLSAVIEMVAVTAFAANLLLTLKQPAGQLRQQTA
                     GG"
     gene            6919..7104
                     /locus_tag="VFQ79_22950"
     CDS             6919..7104
                     /locus_tag="VFQ79_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142599.1"
                     /translation="MIKFLLFCILFVLCWPVALLALFLYPLVWILLLPFRLIGIAVSG
                     VFTLLWVLLTLPVRILR"
     gene            complement(7356..7910)
                     /locus_tag="VFQ79_22955"
     CDS             complement(7356..7910)
                     /locus_tag="VFQ79_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142600.1"
                     /translation="MRFVFVSLCAIAGLFAPRAPAQSEIPPEWDTRKMLETFAGEVKK
                     VKPVLDQVDPKKWTTEGASETYIAQWKSAQAHLEYLVGATEKLASNPERFPIALETYF
                     RLESLETMINSLAQGIRRYDNPAVADLLQSTLAESQPYRQNLQRYMIDLAKVKEQEFR
                     IADSEAQRCREALSRRPAGTQGKK"
     gene            8127..8411
                     /locus_tag="VFQ79_22960"
     CDS             8127..8411
                     /locus_tag="VFQ79_22960"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU0142601.1"
                     /translation="MRPRKGQVVPKEWQLQEAKARFSEVFRLARERGPQRVTKHGREA
                     VVILPAEEYARLARSETRKGSLAEFFAASPLRGSGIDLDRPRDFGREIKL"
     gene            8408..8830
                     /locus_tag="VFQ79_22965"
     CDS             8408..8830
                     /locus_tag="VFQ79_22965"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0142602.1"
                     /translation="MNGFLLDTNVISELIKSEPDGNVLRWIDETEETILFLSVLTLGE
                     IRAGIERLNPGKRRGRLESWLTVDLRRRFQDRILAINEAIAERWGTLSATATKKGRPL
                     PVIDGLLAATALHHNLILVTRNGTDVSGTGIPTLNPWQ"
     gene            complement(9112..9363)
                     /locus_tag="VFQ79_22970"
     CDS             complement(9112..9363)
                     /locus_tag="VFQ79_22970"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEU0142603.1"
                     /translation="MPAFDPGLPAGDGVIGRIRRKHRQTFEQKPNLRTVLWLREVEGY
                     SYEELAEILRIPIGTVRSRLHSGLELPCITGPVNNWRSP"
     gene            complement(9370..9957)
                     /locus_tag="VFQ79_22975"
     CDS             complement(9370..9957)
                     /locus_tag="VFQ79_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142604.1"
                     /translation="MASKHSIARYRNLYAKLLRLYPKPYRERFGEGMEQTFSDLLRER
                     RDGAQGLFAFALWMFADTSAGILRENMTFIQNRLIVWKAGTPVYRAALALALAPAFLL
                     VWFYGAVAEPGDSPGPMFLGPVAVLTIGALIARFQPRGMTYALFATALAQGLFAVIAM
                     ISWGQYLEISILNGFFITLWVGSAILFRRATRPQA"
     gene            complement(9947..>10243)
                     /locus_tag="VFQ79_22980"
     CDS             complement(9947..>10243)
                     /locus_tag="VFQ79_22980"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0142605.1"
                     /translation="STQERHGYGIMKQVEADSQGKVKMGPGTLYGSIGRMIDAGLIRE
                     SDRKIGPEMDDERRVYYKITGLGQKALAAELERYRDVVAVARRRRLSPTTSYGI"
BASE COUNT         1989 a         3026 c         3135 g         2093 t
ORIGIN      
        1 cgccaccatc gtgcacgcgc tgaagccgcg ggtggccgtt atgaacaatg gcgcgaagaa
       61 gggcggaagc cccgaaacct gggacgtcgt ccgcaagtcc cctggactgg aagacctctg
      121 gcagctccac ttcgccatcg ccggcgggga ggcgcataat tcgccccagt cgttcctcgc
      181 caatctccac gaacgctgcg aaggcaactg gatcagactg tccgcgcaac cggacggcag
      241 ctttacggtc ttcaattcgc ggaatcgcta tcagaaaacg tatccggcgc ggtgacacac
      301 ccgttccagg gaacttccgc cgctttccgc tcgtaaatcg tcttgaagtc ccagtcgcgc
      361 ggacttcact ttatgatcgg gctcatccac cagacattcc tcaacatgtg gcgtcacaag
      421 ctgcgaagtt tcctcaccat gttcggcatc gcctggggaa tctgctcgct ggtgttgatg
      481 ctggcgctct gcgacggttt ccgccaggga cagcgcaaga acatgtccca gattggcgac
      541 aacctggtct ttctttacag cggacgcacg gaacgtcagg ccggcggcga gcgcgccgga
      601 aggcgaatct ggctgacgga acagggggta gaggccgtac gggccaattg tccgctggtt
      661 gagacggccg cgccggagat caagcgttac gatacgccgg tagccagcag ctacaattcg
      721 ggccgttttc tggtgctcgg ggtctcgccc gaatatctga agctacgcaa tcttccggtc
      781 gagaccggac gccccgtgca ggcacgcgac gtcgaagaga ttcgccgcgt ctgtgtgctc
      841 ggcgcttccg tccggaagca gttgttcgaa gagcgcctgc cgctcggagg cgagatccgc
      901 atcaacggct atccctacac ggtcatcgga gctatgagcg agaagatgca gaacagctct
      961 tatgacggtt gggacaacga taagatcctg atcccgataa cgagcctgcg gcgcgactgt
     1021 cctccggagc gcgccacgta ccgcccggac cgcgtggatt ccatcctgta ccggccgttg
     1081 tcggtgaaag aatggagagc cacacaggta caggtgcgcc gtgcgctagc cgccgtctat
     1141 gatttcgatc cgctcgatga aagcgccgtg ccggtgaacg actccgtaga aggagccgag
     1201 ctgttcgacc gtgtattcga ttccggcgag atgttcctgg cggtgattgc gctgatcacg
     1261 ctatcgctcg gcggcgtgag tgtgatgaac acgatgatga tggccgttgc cgagcggacc
     1321 ggcgagatcg gcctgaagaa ggcgctcggc gcgacccgtc atcgcattct cctcgatttc
     1381 ttcctcgagg gggtcttcct ggcgctgctc agtggagcgg ttggcattgg agcggtctgg
     1441 acgctggcgg gcgtcgtcaa ctcattgccg atgccggcga tgttttccgg actgctgatc
     1501 gatatgaaga gtgctttgct ggcgctggcc gcgctcggta cggtggccat ctgctccgcc
     1561 gttcctccgg cgtggcgcgc cgcgggactt actccggtcg aagcgttgcg ggaggagaga
     1621 taacatgacc gcgcggattg tgctgaaaga agccttcgca tcgctccggc attaccggcg
     1681 gcgcacgact gtaaccgtgc tcagccttgc ctggggtgtc gcttccttcg tcatgttgat
     1741 gtcctacggg gacggcttcg atgaagcaat acggcgtgcg ttcctggcgg tgggtcagga
     1801 cctgatcatt atgagcggcg gccaaaccag caaacaagcg ggcggattgc gttcgggacg
     1861 ccgcatccgg ctcgaactcg atgacgtcaa agcgattcgc gagagcgctc ccgcagtgtt
     1921 gacgctctca ccagaaatct tcggacgaaa catgaacgta gtccgcgggc cgcgcgagaa
     1981 gcagtatttc ggcagggccg tctggccgga atacgggcgt attcggaatg tgaatctggt
     2041 ggcaggaaga tggatcaacg cagaagataa cttgaaccgc aaccgcgtcg tggtactggg
     2101 atcgaccgtg gctaaggaat tattcagcgg catcccgccg gtaaacgaag aggtgacagt
     2161 gaacgggctt cgcttcacgg tcgtcggcgt gatggattcc aaagtccagc ttgcgaatta
     2221 taaccgccgc gacaacatgt gcttctttct tccttatgaa acgatgtcgc tgttcgcgga
     2281 cgtacgctac ccgcacttga tcctctggat gcccgtttcg ggcatggccc ggcagacttc
     2341 gatcgaccag gtacggacaa ttatggcgac gctgcaccgc tttcagcccg acgacagcaa
     2401 agcagttgag atcctcgcct tcaaccagtt tttgagcatc atcgacggca tgtcgctcgc
     2461 ggtgaaattg ttgttgggtt tcgtcggcgt attgacgctc agcatcggcg gtgtcgggct
     2521 ggccaatgtc atgctggctt ccgtgatcga acgtacgcgt gagatcggtg ttttaaaagc
     2581 cctgggagga cggaagaaga cgattcttgg acaatttctg gctgagtcgc taatggtgat
     2641 ttcgaccggc ggttttctgg gcgtccttat cggaacggct gccacgaaag cggttggggc
     2701 catgcctctg ttcgaatcca ttttccagga cgcagcggac aaaggaagcc tgcaactgca
     2761 tgtttccatg cccgcgattc tgatttcgtt gggcgtgctg ctggttgtgg gtctcattgc
     2821 ggggatgatt cccgcgatca aagccgcgcg tctcgacccg attgaagctt tgcgatacga
     2881 gtaagcccac ccatctacta agctggtatc tgcgcccgcc ggcttatgaa cgattcttcc
     2941 ccatcagggg gtaaacccgg ttccgaacac actaccggcc gctttgcgct ttttgtcgcc
     3001 gcgggtatcc ttctcagccg tgtggtgggg ttggtgcgtc tcagagcctt tgcttattac
     3061 ttcggcgttc agtctgatgc ggcggacgcc ttcaacgccg gctttcgtat tcccaatctc
     3121 ctgcaaaacc tgttcggaga aggcgtgctt tccgcctcct tcattcccgt gtatgcgtcg
     3181 gcgctggccc ggggcgacga aaaagaagcg ggccggcttg ccggcgccat tgcggcgatc
     3241 ctcagcctga tcacctcggc cgtagtcctg gccgggattt ttgcggcgcc cgttctggtc
     3301 gatcttattg cccccgggtt tcagggtgag aagcgggagc tgacggtgcg ggtggttcag
     3361 atcctgttcc cgggtgcggg attgctggtg ctgtccgcat ggtgcctggg cattctcaac
     3421 agccacgggc gcttttttct ttcctacgct gctccggtga tctggaatgc ggcgatgata
     3481 gccgcactgg ttggttttgg cgggcgcgtc gatcagccgc ggctggccat tgcgctcgcc
     3541 tggggatcgg ttgccggcag cctgttgcag ttcgccgtac agttgcccgt ggtggtccgg
     3601 ctggcgcggc gtttgcacct gtcactgggg cgcgggatgg caagcgtccg gaccgttctg
     3661 cggaatttcg tgccggtatt cttcagccgg ggcgttgtgc agatcagcgc ctatgtcgat
     3721 gcgatgatcg cgagcctgtt gccaaccggt gcgatgacgg gccttgccaa cgcgcagttg
     3781 ctatacacgc tgccggtcag cctgtttggc atgtccgtat cggctgcgga gcttccggcc
     3841 atgtccagcg cgctgggaac cgaagaggag attgccggtg cgctgcgccg gcgcttgaat
     3901 tcaggactgc ggcggatcgc attctttgtc gttccttccg ctgttgcgtt tgcggcattg
     3961 ggagacgtga tttcggcggc gctgcttcag acgggacgat ttacgtatgg cgacgcggtc
     4021 tacgtatggg gcattctggc cgggtactcc gttgggctgc tggcatcgac gttaggacgg
     4081 ctgtactctt cgacttatta tgctttgcgc gatacccgaa ctccgcttca gtttgcgatc
     4141 gttcgcgtat cgatggcggg tgtgctcggg attgcgttcg ctacgcaatt gccgggcatg
     4201 atgggcgtcg aagagcgctg gggagtcgcc ggattgacgc tcgcctcggc gatcgcgggc
     4261 tgggtggaat tcgcactgct gcgccaccgc ctgaatcgcc ggatagggga aaccgggctg
     4321 ccccttgcct tcagcattcg cgtctgggtt gcagggctgc tggccgcggc cgccggatgg
     4381 ggtgtgaagc ttgctgtagg cgcggctgga ccgctagtgg cggcggcgct catcctgccc
     4441 gcgttcggca tcgtctactt cggaatgacg ttcgccttgg gtattccgga agcgcgggcg
     4501 tggttccgcc gcgtgcgccg ctgagatcgc ggcaaacgtc ctaaaatcag gcaagggggc
     4561 atatgttgac taccagcgtc ctgaagaaag gaacgcgcct cattctcgat gtactggggc
     4621 caaccatcga gtttctgacc tcaccggagg aagccgatgc ttgttgcatg ctgatcggga
     4681 ccatcccgcc gggcggtgtg atccccctcc accaccacgc cgattttgag gatttttacc
     4741 agctctccgg gactgtccag gtgatatcgg aacaaaacgg cacactcgaa tggttggacg
     4801 taacgccggg cgacttcgtc cgggtgcccg gcaacgccaa acacgcgttc cggaatctgt
     4861 cgaacgagcc ggttgtgcag ttgattgtaa ccacccccag attggggaga ttcttccagg
     4921 agctcgggaa gccgtttgtc cccggggcgc ctccacgaaa gccggcgccc gatgtcctcg
     4981 agcatttcct gcgagtctcc gctgactacg gctactggaa cgcgagtccg gaagaaaatg
     5041 cggcgatcgg aatttctttg cttggatgac ggcgtgcgcg gcagtgcgga ctacacagcc
     5101 attcgcgccc ggaacggcaa agccacgact aaccccgcca caaggccaac ggcattcgca
     5161 atcatgtcat gcagatcgaa catgcggtcg ctgaagtagc tctggccgaa ttcgagaagc
     5221 actccgaggg agagcaggcc cagaatcagt ccagccagca tccagcggtt ctcatggaga
     5281 gccggcagaa acgacagtac cgcataagcg gccgagtgca gcagtttgtc actcaggtgg
     5341 agaagatcga tcatctcgat aggcggcgac gaggagggca gtagcgatcc cacgacgacc
     5401 cccgacactg ccaggaacca cagcacacgc aaacttcgtc tcgagagtcg ttttttgtac
     5461 attgcccagc aacgattcta gcgttagccg ccggcggttt gctgccgtag ctgtcccgcg
     5521 ggttgtttca acgtcagaag caggttggcc gcgaatgctg tcaccgcaac catctcgatg
     5581 acggcggaga gcggcagcag cggccagagc caaggcaggt gtccttcgta tgcaccgatc
     5641 tcggagccga ctcgcaagac gcacccggcg tttaataacg ccagggcggt gaacatgaga
     5701 gccgggctga agaggttgcg cattccgcag aagcccggca gaacacgctg cccgatggcg
     5761 aagaccattg tcgaaatata gccgacggtc agtgcatgcc gcgaggctcc ccaaatgccc
     5821 ccggacgtgt cccagacagg cgcggccaac gagatcacgg ctgacagcag cagccagaca
     5881 taagcgatac gcacgaaagt tgggaagctc cggtgcactc cagcaagatt cgcgggaccg
     5941 gtagcgggag caaacacggc cagcgcgaca gcggccgcga ctgccccgag caaaaacaat
     6001 cccgcggcgg caatccactc tccggcgagc gcgccgaaaa cggcagcggt gttgatggcc
     6061 agtgcggcca acagcaaggc gccattggcc tgacggaggc cgagaaagcc gggcagccag
     6121 cgcgcgctga aaccccagat gttcactacc gggaacgccc acgtaaacag caccagcaag
     6181 cgctgattcg gaacgtgcgc tatcgccgga ccgctgccgt cgatcgcgct ttgaatggca
     6241 aacgccaggt ttgccagtaa gctcaccagg aaaccgatgg tgcccgcgaa cacgacgatc
     6301 atccagacgt gggattgcgg gcggtcttcg gtgcggattt ttgggcggcg atgacgccgg
     6361 acggtcagga agaagatcag aaacccggat aattccagca gggccgatgc cggcagcatc
     6421 catcgccact gccacggcca caggtttgtg ctccagcaca ggaaaacacc ggcggtccaa
     6481 caggcccaac tggcccatgc gcggcgggcc gcaaagccgg ctggtccgcc catcttcgaa
     6541 agcgagtgga aaccgattcc cagaatgaaa gagccgatcc agccgaaaat ctgggcatgt
     6601 ccgtgagctt gcagccaggc cggatcaagg ccggcggcgc cctgcgcgtc cgtgatgacg
     6661 ataagattcc aggcgccaag gaacgtcccc ggcaacagca ggaagaagag tccgcctgca
     6721 atccaggcga ttgcgcgctg gagcgccgat tcgtgattcc tgatcgattg cgcatcgagc
     6781 gtcagcgcac acctccgcgt aggaatcagg atagcgggca taccggcggg ccacagtgac
     6841 aaggatcaca tcggcacttc acaagcgtga acttttccgg tggtttcctc gttatgtagg
     6901 ggtggcgagg aagaacaaat gatcaaattc ctgctctttt gtatcctttt tgtgctgtgc
     6961 tggcctgtcg cgctgctggc gctctttctc tacccgttgg tttggatttt gttactgccg
     7021 ttccggctca taggaatcgc tgttagcggg gtgttcacgc tgctgtgggt cctgctcact
     7081 ctcccggtca ggatcctccg ctgacggctg ctcttccgat ggaggggcgg gccgggagtc
     7141 cgccggctcg tcctgcggct caggcgcagc ggcttcggac gccggttcag ggggcagttc
     7201 cgtttcctcc tccaaaggga cttcgcactc gcagcagtcg ctacagcggc ggcacttcgc
     7261 gcacaagtgg tttggacagg cgtccttcgt acagtaaata cagatgcccg tggagggctc
     7321 accgcatatc gtgcagatgt aggtggctac ctcgctcatt ttttcccctg tgtccccgcc
     7381 ggtcgccggc tgagagcctc ccggcagcgc tgggcttcgc tgtcggcgat acggaactcc
     7441 tgttccttca ctttggctag atcgatcatg taacgctgca ggttctggcg atagggttgg
     7501 gattccgcca gcgtgctctg aagaagatcc gccacggccg ggttatcgta ccgtcgaata
     7561 ccctgtgcca gcgaattgat catcgtctcg agcgattcca ggcggaagta ggtctcgagc
     7621 gcaatgggaa accgctcggg gttgctcgca agtttttcgg tggcgccgac gaggtactcc
     7681 agatgggctt gtgcggattt ccattgggcg atataagtct ctgaagcacc ctcggtggtc
     7741 cacttcttcg gatcgacctg gtcgagcacg ggcttaacct ttttcacctc tccggcgaac
     7801 gtctcgagca tcttgcgcgt gtcccattcc ggagggattt cggattgagc cggagcccgg
     7861 ggcgcaaaaa gaccggcaat agcgcagaga cttacaaaaa cgaatctcat aggtcgcaag
     7921 aggccgaagc cccattaaac cccagtttcg cacgccgggg cgggagaagt tggtccggtt
     7981 cgcttcactc gttgcatgca ggtaacaact tccgctttca ggtaacatca ctaggcggcc
     8041 ccatcgccat gcgctataat ctgactagaa tggtcagaaa ggaaatcccg atgaacagca
     8101 cgcgctcccg aggccggcga gaagatatgc ggccccggaa gggccaggtg gtcccgaaag
     8161 aatggcaact tcaggaggcc aaagcacgct tcagcgaggt gtttcggctc gcccgggagc
     8221 gcggtcccca gcgcgtcacg aagcatggcc gggaagccgt tgtcatcctc cccgcggagg
     8281 aatacgcgcg ccttgcgcgg tccgaaacac gcaaaggaag ccttgccgag ttttttgcgg
     8341 catcgccact ccgcgggtcg ggcatcgatc tcgaccggcc acgcgatttc ggaagagaga
     8401 tcaagctgtg aatggctttt tgttggacac gaacgtaatc tccgaactga tcaaatcgga
     8461 acccgatggc aatgttctcc gctggatcga cgaaaccgag gagacaattc tgttcttgag
     8521 cgttctcact ctcggagaga tccgggcggg tatcgaaagg ctcaaccccg ggaagcggcg
     8581 cggcaggctg gagtcgtggc tcacggtcga tttgcgccgc cggtttcagg accggattct
     8641 agctattaac gaagcgattg cggaacgatg gggcacgcta tccgccacag ccacaaagaa
     8701 aggcagacct ctaccggtca tcgacggcct gctggcggcg accgctttgc atcataattt
     8761 gatactcgtc accagaaacg gcacagatgt cagcgggacc ggcattccga ccctgaaccc
     8821 ctggcagtaa accgcctcgc acaattggcg ctacccgccg gttcccgcgc ctgccacgtc
     8881 cgttctcagc aggagggtcg aacgaagcaa ctattctccg agccgcaccg tcccagcctg
     8941 gcagcgctgt cgcaccgccg cctgacatcc cggccaacac acggccctct gaaatcaaca
     9001 caggcccgcg catggagagt tggtttgacg gcggctccaa cgggatatca aaagcgattt
     9061 tgggctgctg ctcataaacc tcgcctttca gatagaccgc tccacctcct tctatgggga
     9121 tcgccagttg ttgaccgggc ctgtaatgca cggcaattcc aaccccgaat gcaggcgcga
     9181 ccgcaccgtg ccaataggaa ttctcaagat ttccgccagt tcctcatacg aatagccctc
     9241 cacctcgcgc aaccacagca ccgtgcgcaa gttcggtttc tgctcgaacg tttgccggtg
     9301 cttccggcga atgcggccga ttacgccgtc accagccggc agccccggat cgaatgccgg
     9361 caaatgactt caagcctgtg ggcgcgttgc gcgtcgaaag agtattgccg atccaaccca
     9421 tagcgtgatg aagaatccgt tcaggattga gatctccaag tattgtcccc agctaatcat
     9481 cgcaattacg gcgaacaatc cctgggcgag ggctgttgca aacagtgcgt atgtcatccc
     9541 tcgcggttga aagcgtgcaa tgagggcacc gatggtgaga acagcgactg gtccgagaaa
     9601 catcggaccg ggcgagtctc ccggttccgc tacggcacca tagaaccata cgagtaggaa
     9661 cgctggcgca agcgcaagcg ccagggcggc tcggtacacg ggtgtacccg ccttccacac
     9721 aatcagtctg ttttgtataa aagtcatgtt ctctctcaga attcccgcag atgtgtcggc
     9781 gaacatccaa agagcgaaag cgaataatcc ttgtgcccca tctctgcgct cccgaagaag
     9841 atcgctgaag gtctgctcca tcccttcccc aaagcgctcg cggtacggct tcgggtagag
     9901 gcgaagcagc tttgcgtaga ggttccggta ccgcgcgatg gaatgcttag atgccatagg
     9961 aggtggtggg cgagagtctt ctgcgccggg caaccgcaac gacgtcgcgg tatcgctcca
    10021 attccgccgc gagcgccttt tggccaaggc cagtgatctt gtaatagacc cgccgttcgt
    10081 cgtccatttc cgggcctatc ttcctgtcgc tctcgcgtat caatccggcg tcgatcatgc
    10141 ggccaatcga tccgtagagc gtgcccggtc ccatctttac cttcccctgc gaatcagctt
    10201 caacttgctt catgattccg taaccgtgcc gctcctgcgt gga
//
LOCUS       DASTMK010000557        14379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_637011, whole genome shotgun sequence.
ACCESSION   DASTMK010000557 DASTMK010000000
VERSION     DASTMK010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14379
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637011"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..945)
                     /locus_tag="VFQ79_22985"
     CDS             complement(61..945)
                     /locus_tag="VFQ79_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142606.1"
                     /translation="MPASKTSAKSEGVEVRSLTKHFDRYAREQDHVFEIEDEVSRNGL
                     VPNLQIQLPERLGGGSVSTRVTGTFAISFFQADAFAILGLDARISSFEVPAAGKDHPL
                     RTGCLRVALAPDCESGHLESNAPPLNTGIVHRSSGAVRISLGARVQSQELLRRRIPPA
                     RFYLQQSGFLDAASGRLVLSGTGVMIGGLCAGMTFFVGMSCKKFAAVRVVFIDETVQA
                     GRTIQASVTCSPPAAVDLMASLRRDVSVVVPVAPATLATGNGRVSVTVPAGTRTGTKL
                     VIKADSPDDFDEDIATVA"
     gene            complement(1033..1425)
                     /locus_tag="VFQ79_22990"
     CDS             complement(1033..1425)
                     /locus_tag="VFQ79_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142607.1"
                     /translation="MLPELKVVLSFLTMGVYTASLYRNRGGILRFGARGGLYHRALRP
                     SRSRAPVLRFSTLPVRQRHFASVALAIFDGGPSDTAALLIEKTGSFDLAILVAGVSLL
                     VGVAAYLFLLQERGVTLFPLDEGTCGSA"
     gene            complement(1455..2660)
                     /locus_tag="VFQ79_22995"
     CDS             complement(1455..2660)
                     /locus_tag="VFQ79_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142608.1"
                     /translation="MPLGDVLRFSVQALRANKVRTFLTALGLVIGNASVILVVTISLT
                     SRDYILEQIRGIGSNLVYAYYESGGQDATIVEADYVKMADVEAVRQQLGSRIVAATGV
                     MTSFDRMLIEGRERDVRIIGSDEFYPVVRNLQLLAGRFMDGGDVQLRQKVALLTESLA
                     ARLYGSQSGAVGQVLKIHGLQFTVIGTFKERTSTFGLSELSNETVLIPMSVLRYFARV
                     ERIDPMYVQVRRSGDVEAIAGVVKQILESRHRPGAGYKVETLTAILEAANSIALVLTL
                     VLIMVSAIALIISGIGIMNIMLVTVTERTREIGVRMAVGASRKAVLQQFLAEAILISV
                     AGGFAGIVVGVGIPLSVRFLAEGVAVPISGTAIVVAFAVSLLVGLVFGMLPANRASRL
                     NPTEALRYE"
     gene            complement(2664..3515)
                     /locus_tag="VFQ79_23000"
     CDS             complement(2664..3515)
                     /locus_tag="VFQ79_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142609.1"
                     /translation="MKRLAAILTLAAVSLAAADGLDRAQALYNRTDYAGALKVLSASK
                     SGDAGTWALMGKSYFMLGEFKKASEAFEKAVKANPRSSEYHHWLGKAYGRRAETASVF
                     TAPGLAGKTRDEFETAVELDPNNLEAINDLFEYYLEAPGLLGGGLDKASDLTARIAKL
                     DPVEYHWAQARLAEKQKKFGTAEEQLRRAAELAPRQVGRVLDLAKFLAKQGKYRESDA
                     AFERAESLAPGSPKVMFERASTYIRTKRNLEVARELLKQYLESPLTPSDPARHEAERL
                     LRQASGG"
     gene            3677..4825
                     /locus_tag="VFQ79_23005"
     CDS             3677..4825
                     /locus_tag="VFQ79_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU0142610.1"
                     /translation="MISPARQVAFDVLRRVEDGAYASDLLLGASIRLESRNAGLAAEI
                     VFGTLRFQVQLDYLMEYYAGRSGLRLDAEVRIALRMGIYQLRYLERIPRHAAVGESVE
                     LVKRARKRSAAGLVNALLRKVTRDPVVWPDRAIRLSQPEWLLRSWERQFGGEAAERIA
                     EAFLRPPETFVRISGPVPDGQELEPAEIPGGYRVAGATPPGARIQDIGSQSIVPLLDL
                     KPGLKLLDLCAAPGNKTAQALESGAQVIASDIHASRLRALDGMDCERLVLDAAQPLPF
                     RDRFPRILVDAPCSGTGTLGRNPEIKWRLSPQRVAELGRLQAAILRNALSALASGGVL
                     VYSTCSLEREENEAVIEEVLGSSPVRLHRRIPGVQPGDGFFAAVITSN"
     gene            4833..5507
                     /gene="rpe"
                     /locus_tag="VFQ79_23010"
     CDS             4833..5507
                     /gene="rpe"
                     /locus_tag="VFQ79_23010"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU0142611.1"
                     /translation="MVEILPSILSADFAHLGEEIAAVERAGARMLHVDIMDGHFVPNL
                     TLGPPVVKSVRKVTKLTLDLHLMMTDPDRYAPVFIEAGADQISVHQEVCPHLDRTLRM
                     IQSEGAQAGVVLNPSTPVSTLDEVLDVLDYILIMSVNPGFGGQQFIPNALRKVRALER
                     KRKELGLQFAIEIDGGISLENVADAVRAGCDWIVAGSSIFHTDDPTDTFVRIEQAARE
                     ATSIKV"
     gene            5528..6646
                     /locus_tag="VFQ79_23015"
     CDS             5528..6646
                     /locus_tag="VFQ79_23015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU0142612.1"
                     /translation="MNRIRTVLVLFAIAGLLSSCGFRRHKYDNPITKDTEQPDKILYD
                     KAIGDIEKSRFDIARLTLNTLISTYDTSEYLAKAKLAIADSWFREGGSHALAQAEAEY
                     KDFILFYPTLEESAEAQEKVCMIHFKQMEKADRDNTHAMRAEEECRQLLVQFPNSKFA
                     PRAQQRLRQIQEVLAEAEYRVGTFYHAKGSNPAAANRLEGVIGHYPLYSRADEALWQL
                     GDAYAKMGPRFRDKTGEAYARIVKDYPLSPLAAQARARLEQLELPVPEADPVAYARMK
                     YELENRDEPGMMSHFWGIFRKSPDTSAAAKSGSPAMTSLRPSIPASVPLPGTGPGTGV
                     QADVTVSTVEGTSALDTQPDARQGTSAPKGPVPPAPVK"
     gene            6643..7308
                     /locus_tag="VFQ79_23020"
     CDS             6643..7308
                     /locus_tag="VFQ79_23020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0142613.1"
                     /translation="MKRILLADDSPHAQRMGERILREEGYEVVSVTDGATALVRLTDV
                     EPDLVIADAALPGQSGFDICRHIKGNPRYRYTRVVLTAGALEPLDEAEARLAGSDAIL
                     RKPFEASVMTETVGTLIEASRFARELLEREGEGAAQRPDTAGNERAMAAAAPAVVAPV
                     RESVPAAFEENAVDSVPEAEMVRAAVTLALDAAFPALVDEITRRVVESMKPQERDYAR
                     KQL"
     gene            7328..7789
                     /locus_tag="VFQ79_23025"
     CDS             7328..7789
                     /locus_tag="VFQ79_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU0142614.1"
                     /translation="MPEVNNAIQQALKEIQTRFDLKNSKSSIELNEKDKKITLASQDE
                     YKLKSVSEIFEQKLVKRKVPLKALTYGTVTPAAGSSVRQEITLQQGIPTEKAREIVKT
                     IKDSKKKVQASIQGDLVRVSGKDRDTLQDVIRLLRDTDFGIDMQFTNYRSN"
     gene            7792..8418
                     /locus_tag="VFQ79_23030"
     CDS             7792..8418
                     /locus_tag="VFQ79_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU0142615.1"
                     /translation="MARRIESLMLHGPAGRLEARLEEPDDEEPVEACLVCHPHPQHGG
                     TMHNKVVHRLARGLRRSGSVVLRFNYRGVNLSEGEYGHGKGELEDARVALKWLRERYP
                     HLPFSLAGFSFGSRIILRLGCSVQTPLRLIAVGFPASYREKIDLAHCTLRKIFIQSTN
                     DEFGSKNDLEPFVGSLPEPKELYWVQAENHFFRGALDPFEETVFGLKK"
     gene            complement(8425..8853)
                     /locus_tag="VFQ79_23035"
     CDS             complement(8425..8853)
                     /locus_tag="VFQ79_23035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0142616.1"
                     /translation="MIKGVRPRLRGSELARVLLVDDDPASRLTLQTVLEAGGYRVDSA
                     ASAAEAFSMLDRDEYELVLSEMQMESPYAGLKVLAHARMMEYKPATALICTEFASPET
                     EEPYLLVAPEDVPGLFTKVADLISGRALRIIARQMRQANN"
     gene            complement(8972..10291)
                     /gene="purB"
                     /locus_tag="VFQ79_23040"
     CDS             complement(8972..10291)
                     /gene="purB"
                     /locus_tag="VFQ79_23040"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU0142617.1"
                     /translation="MIPRYTRPEMGHIWSDENKYRQWLEVELAASEALAELGEVPAEA
                     AALLRQHASCRPERILEIEREVKHDVIAFTTAIAESMASAGHAEASRWLHYGLTSNDV
                     VDTAQALQLKQASAILLRGIDELVGVLRERAFEFRNTVQIGRTHGVHAEPVTFGLKLA
                     LWYDEMRRNRSRFVSAAEDLRAGKISGAVGTFAHIGPEAEERICRRLGLEPAPIASQV
                     ISRDRHAHFLSVLALIAAVMEKIALEVRHLQRTEVREVEEPFSSGQKGSSAMPHKRNP
                     VTCEQICGLARVVRANVQAAFENIALWHERDISHSSVERVILPDSTILADYLLAKTTW
                     LVRGMRVYPERMKRNLEASRGLVFSGQLLLDLAAAGMLREQAYRVVQAHAMQSWEQES
                     DFRAAVEADPAIRNVLSLEQLEQTFSLNRQLRNVEKIYERVFASTTT"
     gene            complement(10361..11155)
                     /locus_tag="VFQ79_23045"
     CDS             complement(10361..11155)
                     /locus_tag="VFQ79_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0142618.1"
                     /translation="MSFHAEAGIVKICVLASSSSGNATFIGTDETRILVDAGLSRKEL
                     FKRLCAIGEEPGRIDAIVVTHEHSDHVCGLVRVAKALGIPVYMTHLTAPCIDWEQYEP
                     KLETFQAGCRFHIGDIEIESFTVPHDAADPVGFSFQAAGVKVGLVTDLGYIPDSIKFH
                     LRGSDVVILESNHDVEMLKVGPYPWFVKQRVMGRKGHLSNDAVADYICMDLDAATGTL
                     VLGHLSEHNNYPDLVHLVATQALRRRSLNARLVIAAPGAASEVLEF"
     gene            11197..12333
                     /locus_tag="VFQ79_23050"
     CDS             11197..12333
                     /locus_tag="VFQ79_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEU0142619.1"
                     /translation="MPSMRPLDSGPKGRLESLDVFRGATIAAMILVNNPGSFQTTYAP
                     LLHAQWNGWTFTDVIFPSFLWIAGVAMTLSFRRRMERGEDRATLLRHVLRRAAIIFAL
                     GLFLNAFPHFQLATLRIPGVLQRIAVCYLAAAAILLYTSARTQLAILVSLLAGYWILM
                     KTVPVPGYGAGVLEPEGNLAQWIDSRVLAGHMWSHTKTWDPEGILSTLPSIATVLFGV
                     FTGHVLRRTGASIRTLLVLAVSGLLLVFSGELMDVWFPINKNLWSPSFAVLMAGISTL
                     VFTACYWLVDIKRIRAWARPFAIYGMNAIAIYVLAGVLADTLSILKVPAGGETISLWN
                     WIYTRMFVPLANPFNASLLFALTFVLVLYAVAYFLYRRGRFIKV"
     gene            12343..14175
                     /gene="ggt"
                     /locus_tag="VFQ79_23055"
     CDS             12343..14175
                     /gene="ggt"
                     /locus_tag="VFQ79_23055"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0142620.1"
                     /translation="MGKAVLVWALAAASLASVQGQSMGPSFERVLISAGGRPATLWPA
                     ARGTRYAVSSMRMQATMAAEHILRQGGNAFDAAVAGQAVLGFVLEGSNGIGGDATLLI
                     YDAKEKKGWSLNAEGTAPKLATIDWYRQHHDGKLPVDDSLLAGTVPGVIDAWYILLSR
                     WGTKSFGEVLAPAIDLAERGFPIPGSRRAYRADHALRKYPTTVKLLGTDRDWKEGEVY
                     RNTDLARTLKRLVEAERQAAGGGREAGLRAARDRFYKGDIAREIAAFSKENGGLFQYE
                     DFASYSAKVEEPVSIEYRGYAVFKNPSATQGPAELFALNILEGYDLKAMGHNSADYIH
                     TSIEAMKLAMADRDTYLGDMDFIQIPYEALLSKRYAEERRKLIEKDKASLEFRPGDAG
                     RYMPDGKSIERPRDLTLSGDGDHEGDTSYIAVVDDERNAVSFTPSLHSGYGTKVAIGS
                     LGFFLNCRGDYFSLVPGHANALEPGKRPRSTLQSTMVLKDGKLFLLAGSPGGDDQCAR
                     TMQTFLNIVEFGMNPQRAIEAPRWSTRAFPSSPFPHTMRPGDAQVESRIPQPVRAELV
                     RRGHKLFVQRPLTIGSNAAILVDWESGVLSAGADPRAHAAALAW"
BASE COUNT         2920 a         4280 c         4392 g         2787 t
ORIGIN      
        1 ggagttagtc tgttcggccc ccgacgctcc atcggcgcgc gcccggccga agcgcccagg
       61 tcaggcgacg gtggcgatat cttcgtcgaa gtcgtcgggg ctgtccgctt tgatgacgag
      121 cttggttcct gtacgggttc ccgccggaac cgtgacggaa acacgtccgt tacccgtggc
      181 cagggtggct ggcgcgaccg gtacaacaac cgatacatct cgccgaagcg acgccatcag
      241 gtcaaccgcc gccggtggcg agcaggtcac ggacgcctgg atagttctgc ctgcttgaac
      301 ggtttcatcg atgaacacta cccggaccgc cgcgaatttt ttgcacgaca tacccacaaa
      361 gaacgtcatg cccgcgcata acccgccgat catcactcca gtgccgctga ggaccagacg
      421 accgctggcc gcgtcaagaa agccggactg ttgaagataa aaccgagccg gcggaatgcg
      481 tctccgaagc aactcctgcg attgcaccct cgcgcccaaa ctgatccgaa cagctcccga
      541 acttcgatgg acgataccgg tattcagggg tggagcgttg ctttcgagat gtcctgactc
      601 acaatctggc gcaagagcca cacgcaggca gcccgtccgc aatggatggt ctttgccggc
      661 ggccggtact tcgaaagacg agatccgcgc gtcgagtccg aggatggcga atgcatccgc
      721 ctggaaaaac gagatcgcga acgtgcctgt aacccgggtc gagactgagc ctccgcccaa
      781 ccgctccggc aattggatct gaagattggg gaccaacccg ttccgggaca cctcatcttc
      841 gatctcgaag acgtgatcct gttcccgagc atagcgatcg aaatgcttgg tcaaagagcg
      901 tacttcgaca ccctcggatt ttgcgctggt tttgctggcg ggcatcgcat ccctctttct
      961 ttggtgatca gccaagtcgc gtagccgtga cactccccgg cgttgcgcgt atgtgcgcta
     1021 aatataccga cactatgcgc tgccacaggt accctcgtca agcggaaaaa gcgtcacacc
     1081 ccgttcttgc agtaaaaaca ggtacgccgc gacacccacg agcagagaga cgccggctac
     1141 gaggatcgcc agatcgaagc ttcccgtctt ctctatcaga agagcagcag tgtcagaagg
     1201 accgccgtcg aagattgcga gagcaacaga agcaaaatgg cgctgccgga caggaagcgt
     1261 gctgaaacga agtaccggcg cacgtgaacg ggacggccgg agcgcgcgat gatacagtcc
     1321 gccgcgcgcg ccgaagcgaa gaatgccacc ccggttccga tatagggagg ccgtgtagac
     1381 gcccatcgtc aggaaggata gaaccacctt caattcaggc agcacattac gccgctatgg
     1441 caaaccgcca tgacttattc gtaacggagt gcttcggtgg ggttcagtcg tgaggcgcgg
     1501 ttggcgggca gcattccaaa gacgagccct accaggagag acacggcgaa cgcgaccacg
     1561 atggcggtgc ccgagatggg gacggcgacg ccttcggcca gaaagcgcac actgagggga
     1621 atgccgacgc ccaccacgat gccggcaaag ccgccggcga cgctgatgag gatggcctcg
     1681 gctagaaact gttggagcac cgcttttcgc gatgcgccca ccgccatgcg gacgccgatt
     1741 tcccgcgtgc gctcggttac cgtgaccagc atgatgttca tgatgccgat gccggaaatg
     1801 atcagcgcaa tggcggaaac catgatcaat accagcgtca gcacaagcgc aatgctattg
     1861 gcggcttcga ggatggcggt gagcgtttct actttgtagc cggcgccggg acggtggcgg
     1921 ctttcgagga tctgcttcac cacacccgcg atcgcctcta cgtcgcccga gcgccgcact
     1981 tgcacataca tcgggtcgat gcgctccaca cgcgcgaaat agcggagcac gctcatcgga
     2041 atgagcaccg tctcgttcga taactccgag aggccgaacg tggacgtccg ctccttgaac
     2101 gttccgatca ccgtgaactg gagcccgtgt attttcagga cctgtccaac ggccccggac
     2161 tggctgccgt atagccgtgc ggcaagggat tcagtaagca acgccacctt ctggcgcagt
     2221 tgcacgtcac cgccatccat gaaacggccg gcgagcagtt gcaggttgcg gaccacagga
     2281 tagaattcgt cggagccgat gatccggaca tcgcgttccc ggccttcgat cagcatgcgg
     2341 tcgaagctgg tcatcacccc ggtggccgcc acaatgcggg agccgagttg ctggcggacg
     2401 gcttcgacat cggccatctt cacatagtcg gcttccacaa tcgtggcatc ctgtccgccc
     2461 gattcgtagt aggcgtagac gaggttggag ccgatgccgc gaatctgttc caggatgtaa
     2521 tcgcggctgg tcagcgagat tgtgaccacg aggatgacgg aggcattgcc gatcacaagc
     2581 cccagggccg tgaggaaggt ccggaccttg ttcgcgcgca gcgcctgtac gctgaaccgc
     2641 agcacatccc cgagaggcat gaatcagccg ccgctcgcct gccgcaggag ccgctccgcc
     2701 tcatggcggg ccgggtccga cggtgtgagc ggagactcga gatactgttt caacagctcc
     2761 cgcgcgactt ccagattgcg cttggttcgt atgtaagtgc tggcacgctc aaacatcact
     2821 ttcgggctgc cgggcgcaag gctttcggcg cgttcaaacg cggcgtcgct ttcccggtac
     2881 ttaccctgct tggccaggaa tttcgccaga tccagtacac gccctacctg gcggggcgcc
     2941 agttcggcgg cgcgccgcaa ctgctcttcg gccgttccga acttcttctg cttttccgcc
     3001 agcctggctt gtgcccagtg gtattcgacc ggatcgagtt tcgctattct agcggtgaga
     3061 tccgaagctt tgtccagccc gccgccgagc aggccgggcg cttccaggta gtattcgaaa
     3121 agatcgttga tggcttccag gttgttgggg tccagctcaa cggccgtttc gaactcatcg
     3181 cgcgttttgc cggcgagccc gggagcggtg aacacgctgg ccgtttccgc gcgccgcccg
     3241 taagccttgc cgagccagtg atgatactcg gagctgcgcg ggttggcttt cacagccttt
     3301 tcaaacgcct cggacgcctt cttaaactct cccaacatga agtagctctt acccatcaag
     3361 gcccaggttc cggcatcgcc ggacttggag gctgagagca ccttcagcgc tccggcatag
     3421 tccgtccggt tgtacagggc ttgtgccctg tcgaggccgt ccgctgccgc cagggacacg
     3481 gccgccaggg tcaaaatggc tgctaacctc ttcatttccg gtaaccgacc ctccacctag
     3541 tgaatgctaa catcgtatct gaagttttag atgcgggcaa ggtcttcgag ggtacgggaa
     3601 acattgacgt tctcccactg atagaacgcg tcccggccgg ccatttcggc tcaaaaacgc
     3661 gaggaatttt ccaaacatga tctctccggc gcggcaggtg gcgtttgatg ttctacgccg
     3721 tgtagaagac ggagcatatg cctctgacct gttgctcggc gcatcgatca ggctggaaag
     3781 ccggaatgcc gggctggcgg cggagatcgt cttcggcacc ctgcggtttc aggtgcaact
     3841 cgactacctg atggagtatt acgccggacg cagcggactt cgcctggacg ccgaagttcg
     3901 gattgcgctg cgcatgggga tctatcagtt gcgatacctg gaacggattc cccggcatgc
     3961 cgcggtaggc gaaagtgtcg agctggtgaa gcgggcgcgc aagcgctccg ctgccgggct
     4021 ggtgaacgcc ctcctgcgaa aagtaacgcg agatcccgtg gtgtggcccg accgcgccat
     4081 ccggctttcc cagcccgagt ggcttcttcg aagctgggag cggcaattcg gaggggaagc
     4141 tgccgaacgg attgccgaag cgtttttgcg acctccggaa acgttcgtgc ggatctccgg
     4201 accggttccg gacgggcagg agttggagcc ggccgaaatc cccggcggtt accgcgtagc
     4261 cggagcaacg cctccgggag cccgaattca ggacatcggc tcgcaatcca ttgtcccgct
     4321 gcttgacctg aagccgggat tgaagttact ggatttgtgc gccgcgcccg gcaacaagac
     4381 ggcgcaggcg cttgagtcgg gggcgcaggt gatagcatcc gacattcatg ccagccggct
     4441 gcgggcgctc gatggaatgg attgcgagcg gctggtgctg gatgcggctc agccgctccc
     4501 gttccgcgac cggtttcctc gcattctggt ggatgcgccc tgctcgggga ccggaacgct
     4561 gggaaggaac cccgaaatca agtggcggct cagtccgcag cgggtggcgg agctcggacg
     4621 gctacaggcg gccattcttc ggaacgcgct ctccgcgctg gcttcaggcg gtgtgttggt
     4681 gtattccacc tgttcgctcg aacgcgaaga gaacgaagcg gttatcgagg aggtgctggg
     4741 atcgtcgccg gtgcgtttgc accgccggat accgggtgtg cagccgggag acggcttctt
     4801 tgccgctgtg ataacatcga actagccgcc agatggttga aatccttcct tcaattctgt
     4861 ctgccgactt cgcacacctg ggcgaggaaa tcgcagcggt ggaacgagcc ggggccagaa
     4921 tgcttcacgt ggacatcatg gacgggcatt tcgtcccgaa cctgacgctg ggcccgccgg
     4981 ttgtcaagtc tgtccggaag gtcacgaagc tgacgctgga tcttcattta atgatgacgg
     5041 atccggaccg ttacgcccct gttttcatcg aagcgggggc cgatcagatt tccgtgcatc
     5101 aggaggtctg cccgcatctc gaccgtacct tgcgcatgat ccaaagcgag ggagcacagg
     5161 cgggcgttgt gctcaacccg tcgactcccg tctctacact ggacgaggtg ttggacgtgc
     5221 tggattatat tctgatcatg agcgtgaacc ccggcttcgg gggacagcag ttcattccca
     5281 acgcgctgcg caaagtgcgg gcgctcgagc gaaagcgtaa ggaactcgga ttgcaatttg
     5341 ccattgagat cgacggcggt atcagcctcg aaaacgttgc cgatgcggtc cgggccgggt
     5401 gtgactggat cgtagccggg tcgtcgattt ttcacactga cgatccgacg gacacattcg
     5461 tccggatcga gcaggcggca cgcgaagcca cctcgataaa ggtgtaacaa tgaggagcag
     5521 tagcaatatg aatcgaattc gaactgtgct ggtgttgttt gcgatagccg gtttgttaag
     5581 ctcgtgcggt tttcgccgcc acaagtacga caatccgatc acgaaagaca cggagcagcc
     5641 ggacaagatc ctgtacgaca aagcgatcgg ggacatcgaa aaaagccgct ttgacatcgc
     5701 ccgcctcacc ttgaatacgt tgatcagcac gtatgacacg agcgagtacc tggcgaaggc
     5761 gaagctggcg attgccgaca gttggttccg cgagggcgga tctcacgcgc tggcgcaggc
     5821 cgaagccgag tacaaggatt tcatcctctt ctacccgacg ctcgaagaat cggcggaagc
     5881 ccaggagaag gtctgcatga tccatttcaa gcagatggag aaggcggatc gtgacaacac
     5941 gcacgccatg cgggccgagg aagagtgccg gcagttgctg gttcagtttc cgaacagcaa
     6001 gttcgctcca agggcgcaac agcggcttcg tcagatccag gaagttctgg cggaggccga
     6061 atatcgcgtg ggaaccttct atcacgccaa gggcagcaac cccgcggcgg caaaccggct
     6121 ggagggcgta atcggccact acccgcttta tagccgggcg gacgaggcgc tctggcagct
     6181 tggcgacgcc tatgccaaga tggggccgcg cttccgcgat aagaccggcg aggcctacgc
     6241 gcggattgtg aaagactatc ctctaagccc gctggcggcc caggcccgcg cacggctgga
     6301 gcagttggag cttccggttc cggaggccga cccggtggca tacgcacgga tgaaatatga
     6361 actggagaac agggatgaac cggggatgat gagccacttc tggggcatct tccggaagag
     6421 cccggacacc agcgccgcgg cgaaatcggg cagtcccgcg atgacttctc tccggccgag
     6481 cattccggca agtgtgccgc ttcccggcac tgggcccggt acgggagtgc aggccgacgt
     6541 gaccgtctct accgtcgaag gaacgtctgc cctcgatacg cagccggatg cccgtcaggg
     6601 gacttcggcg ccgaaagggc cggtaccgcc tgcgcccgtg aaatgaagcg catcctgctc
     6661 gctgacgaca gcccacacgc acagcgtatg ggggagcgta ttcttcggga agagggctac
     6721 gaggtcgtca gcgtgacaga tggagctacg gcgctggtcc ggctcaccga cgtcgagcct
     6781 gacctggtga ttgccgacgc cgcacttccg ggacaatcgg ggttcgacat ctgtcggcac
     6841 atcaaaggaa atccgcggta tcgatacacc cgggtagtgc ttaccgcggg cgcgctcgaa
     6901 ccgctggatg aagccgaagc aaggctcgcc ggcagcgacg ccatcctcag gaagccgttc
     6961 gaggcttcgg tgatgacgga gacggtcggc acgttgatcg aggcgtcccg gttcgccaga
     7021 gagctattag aaagagaagg cgagggagca gcgcagcgtc cggacacagc cggcaacgaa
     7081 agagccatgg cagcggcggc gcccgcggtg gtggcgcccg ttcgcgaaag cgtgcccgct
     7141 gcattcgagg agaatgcggt ggacagtgtg cccgaggcag agatggttcg cgccgcagtc
     7201 acgctggctc tggatgccgc cttccccgcg ctcgtcgatg aaattacgcg tagggtggtg
     7261 gaatcgatga agccgcagga acgggattat gccagaaaac agctttgaca tcgtatctca
     7321 ggttgaaatg ccggaagtaa ataacgccat ccaacaggcg ctgaaggaaa ttcaaacccg
     7381 attcgacctc aagaactcaa aatcctccat cgagctgaac gagaaagaca aaaagattac
     7441 gctggcaagc caggatgagt acaagctgaa atccgtttcg gagattttcg aacagaagct
     7501 ggtaaagcgc aaggtgccgc tgaaggcgct aacttatggg acggtcacgc ccgccgccgg
     7561 atcgtccgtc cggcaggaga tcacacttca gcagggtatt ccgaccgaga aagcccgcga
     7621 gatcgtcaag accatcaagg acagcaagaa gaaggtgcag gcgtcgatac agggcgacct
     7681 cgtacgtgtg tcggggaaag atcgcgacac gcttcaggac gtgatccgtc tgctgcgcga
     7741 taccgatttc ggaatcgaca tgcagttcac caattaccgg tcgaactgaa catggcgcgg
     7801 cgcatcgaat ccctgatgct ccacgggcca gccggacgtc tcgaagcacg cctggaagag
     7861 cctgacgatg aggagccggt ggaagcctgc ctggtgtgcc atccgcatcc acaacacggc
     7921 gggaccatgc ataacaaagt ggtgcaccgc ctggcgcggg gtctgcggcg gtccggatcc
     7981 gtggtgctgc ggttcaacta ccggggagta aatctcagcg aaggcgagta cgggcacggt
     8041 aaaggcgaac tcgaagacgc gcgcgtcgcc ctaaagtggc tgcgcgaacg gtacccacat
     8101 ctgccgtttt cccttgccgg cttctcgttc ggctcgcgca ttattttgcg gctcggctgt
     8161 tcggttcaaa cgccgctgcg gctgatcgcg gttggttttc cggccagcta tcgcgaaaag
     8221 atcgatcttg cgcactgtac cctgcgaaag atcttcatcc aaagcacgaa tgacgaattt
     8281 ggctcaaaga acgatctgga gccgtttgtc ggcagcctgc cggaaccgaa ggagctctat
     8341 tgggtacaag ctgagaacca ttttttccgc ggtgcgttgg accccttcga ggaaaccgtg
     8401 ttcggactga aaaaatgagg ggcttcagtt attggcctgc cgcatttggc gcgcgatgat
     8461 gcgcaaagcg cggccgctga tcaggtcggc aacttttgtg aacaatcccg gaacgtcctc
     8521 gggggccacc agcagatagg gttcttccgt ctcgggagag gcgaactccg tacagatcag
     8581 agccgttgca ggcttgtatt ccatcatccg ggcgtgcgcc aggactttaa gacccgcata
     8641 cggagattcc atctgcattt cacttagaac cagctcgtat tcatcccgat ccaacatgct
     8701 gaacgcctca gccgccgacg ctgccgagtc aacccgataa ccaccggcct ccaaaactgt
     8761 ctgaagggtt aatctggaag ccggatcatc gtccaccaac aggactctgg ccagctccga
     8821 accacgcaac cggggccgaa cacccttgat catctaccgt gtgccctttc ttccaggaaa
     8881 ctgctgctaa gtgatagttt acccgaagaa tcggcaattc gatccagata tttcagagat
     8941 ccatatctcg aagggggcgg ttctattcta cttaagtagt agtactagca aaaacccgct
     9001 catagatctt ctctacgttt cgcaattgcc ggttgaggga gaaggtctgc tcaagctgtt
     9061 ccaaagacag gacattgcga atcgcagggt cggcttcaac ggcggcgcga aaatccgatt
     9121 cctgttccca ggactgcatg gcgtgggcct gaacgacgcg ataagcctgc tcacgcaaca
     9181 ttccggccgc ggccaagtca agcaggagct gaccggagaa gaccagcccc cggctcgctt
     9241 ccaggttcct tttcatacgc tccggataga cgcgcatgcc ccgtaccagc caggttgttt
     9301 tggcgagcag atagtcggcg aggatcgtgg aatccggcag aatgacgcgc tccacggagg
     9361 aatgagagat gtcgcgctca tgccaaaggg caatgttttc aaacgccgcc tggacgttgg
     9421 cgcgaactac ccgggccaac ccgcaaatct gctcgcaggt gacgggattg cgcttgtgcg
     9481 gcatggcgga actgcctttt tgcccgctcg aaaagggttc ctccacttcc cggacctcgg
     9541 tccgctgaag gtgccttacc tcgagcgcta tcttttccat caccgccgct atcaaagcga
     9601 gcaccgagag gaaatgagcg tggcggtcgc gcgaaatcac ctgagacgcg atgggtgcgg
     9661 gctccagacc caggcggcgg cagatgcgtt cctcggcttc ggggccgata tgggcgaacg
     9721 tccccaccgc gccggaaatc ttgccggcac gcaagtcctc cgccgcgctg acgaaccgcg
     9781 agcgatttct acgcatctcg tcgtaccaga gagccagctt cagaccaaac gttaccggtt
     9841 cggcgtgcac gccgtgggta cgtccgatct ggacggtgtt ccgaaattcg aaagcgcgct
     9901 cgcgaagcac ccccaccagt tcatcgatac cgcgaagaag gattgccgaa gcttgtttga
     9961 gttgaagggc ttgcgccgta tctactacgt cattagaagt taatccgtaa tgtaaccatc
    10021 gggaagcttc ggcatgcccc gcgctcgcca ttgattcagc tatcgctgta gtaaatgcga
    10081 tgacgtcgtg cttgacctcc cgctcgatct caaggatccg ctccggccgg caactggcat
    10141 gctggcgcag aagcgctgcg gcttccgccg gcacttcgcc tagttcggcc agcgcttcgg
    10201 atgcggccag ctctacctcc agccactgcc ggtatttatt ctcatcactc cagatgtggc
    10261 ccatctcggg cctggtatat cgcggaatca tgtcttagta ccctttacat aaccgctacc
    10321 gctcgctcac gcgcgcggct ccgttccgag cggtcccgtt tcaaaactcg agaacttcgg
    10381 acgcggctcc cggtgcggct atcaccagtc gcgcgttcag ggagcggcgc cgcagggctt
    10441 gtgtggcgac caggtgaacc agatcggggt agttgttatg ctcgctcagg tgccccaaca
    10501 ccagcgtgcc ggtggcggcg tcgagatcca tgcaaatgta atccgccacg gcatcgttcg
    10561 acagatgccc tttgcgcccc atcacgcgct gtttcacgaa ccatggatac gggccgacct
    10621 tgagcatttc gacatcgtga ttggattcga ggatgacgac atcactgccg cggaggtgga
    10681 acttaatgga atccgggatg tagcccagat ccgtgacgag tcccactttg actccagcgg
    10741 cctgaaaaga gaaccccacc gggtccgccg catcgtgggg aacggtgaag ctctcgattt
    10801 cgatgtcgcc gatgtggaaa cggcagccgg cctgaaaggt ttcgagcttt ggctcgtact
    10861 gctcccagtc gatgcacggc gccgtcaggt gagtcatgta gacgggtatg cccagagcct
    10921 tggccacgcg caccaagccg cagacgtggt cgctgtgttc atgggtaacg acgatggcgt
    10981 cgattcgccc gggttcttcc ccaatggcgc aaagccgctt gaacagctcc ttccggctca
    11041 ggccggcatc cacgaggatt cttgtttcgt ccgtgccgat gaaggtggca ttccccgaac
    11101 tgctgctcgc cagtacacag attttaacga ttccagcctc cgcgtgaaaa ctcacttgta
    11161 gcacatcgac tcacggcaac gctattcggg agtacaatgc catccatgcg cccgctggac
    11221 agcggtccga aggggcgcct ggaatcgctc gatgtattcc ggggcgccac catcgccgcg
    11281 atgattctgg ttaataatcc cggttctttt cagactactt acgcaccgct gctgcacgct
    11341 caatggaacg ggtggacctt cacggatgtg atttttccat ccttcttgtg gatcgccgga
    11401 gtcgctatga cgctatcgtt ccgcaggcgc atggagcgcg gggaagatcg ggccacgctt
    11461 ctgcgccacg tgttgcggcg ggcggccatc atttttgcgc tcggcctgtt tctgaatgca
    11521 tttccacact ttcagcttgc gacgctgcgt attccgggcg tgctgcaaag aatcgcggtt
    11581 tgctacctgg cagccgcggc gatccttctc tacaccagcg cgcgcactca acttgcgatc
    11641 ctcgtttcgc tgctggcggg ctattggatc ctgatgaaga ccgtgccggt gccgggatac
    11701 ggcgcgggag tcctcgaacc ggaagggaat ctggcgcagt ggatcgacag ccgcgtgctg
    11761 gccggccata tgtggtcgca caccaagacc tgggaccccg aaggaatcct gagtacgctg
    11821 ccttccatcg ctaccgtgct attcggcgtg ttcacgggcc atgtgctgcg ccggacgggc
    11881 gccagtatcc gtaccctgct cgtgctggcc gtatcgggcc tcctgctggt gttctccgga
    11941 gagctcatgg acgtgtggtt ccccatcaac aagaacctct ggagcccgtc atttgcggtt
    12001 ctgatggcgg gcatttcgac gctggtcttc accgcctgtt actggctggt ggacatcaaa
    12061 aggattcgcg cctgggcccg gccgttcgcc atttatggaa tgaatgcgat cgccatttat
    12121 gtgctggcgg gtgtcctggc ggatacgctg agcatcctca aagtgccggc cggaggcgaa
    12181 acgatttctt tgtggaactg gatctacaca cggatgttcg taccgctggc gaacccgttc
    12241 aatgcatcgc tgctgtttgc actgacattc gtactggtgc tttacgccgt ggcgtacttc
    12301 ctgtatcgcc ggggccggtt catcaaagtt taggaggtcc acgtgggaaa agccgttctg
    12361 gtttgggcac tcgccgcagc cagtcttgca tccgttcagg gccaatcgat gggcccgtcg
    12421 ttcgagcgcg tcctgatcag cgccggcggg cggcccgcca ctctttggcc cgccgcgcgt
    12481 ggcacgcgtt atgcggtaag ctccatgcgc atgcaggcga cgatggccgc tgaacatatt
    12541 ctgcggcagg gtgggaacgc cttcgatgct gctgtcgccg gacaggccgt actcggcttc
    12601 gtgctcgagg gctcgaacgg catcggcggt gacgccacgc tgttgatata cgacgccaaa
    12661 gagaaaaagg gctggtctct taacgcggag ggaaccgcac cgaagctggc aaccatcgac
    12721 tggtatcgcc agcaccatga cggcaaactc cccgtcgacg attctctact cgccggaacc
    12781 gtaccgggtg tgatcgacgc ctggtatatt ctgctgtcgc gctggggtac gaagtccttc
    12841 ggcgaggtgc ttgcgccagc catcgatctg gccgaacgcg gattcccgat tccgggctcg
    12901 cgccgcgcct atcgtgcgga tcacgcactg cgaaaatatc cgaccaccgt gaagttgctc
    12961 ggtaccgatc gcgactggaa agagggagag gtctaccgga atacggatct cgcacgcacc
    13021 ttgaagcgcc tggtggaggc cgaacggcag gcggctggcg gaggacgcga agcgggtttg
    13081 cgcgccgcgc gagaccgttt ctataagggc gatatcgcgc gcgagatcgc cgcgttttcc
    13141 aaggaaaacg ggggactgtt ccagtacgag gatttcgcct cttacagcgc gaaagtggaa
    13201 gagcccgttt ccatcgagta ccggggttac gccgtgttta agaacccctc cgccacgcag
    13261 gggccggccg agttgttcgc cttgaacatt ctggaaggct acgacctgaa agcgatgggc
    13321 cacaacagcg cggattacat ccacacctcc attgaagcga tgaaactggc aatggccgat
    13381 cgcgacacct atctcgggga tatggatttc atacagatcc cctacgaggc gctgctgtcc
    13441 aaacgctatg ccgaggagcg ccgcaagctg atcgagaaag ataaagcttc gctcgagttt
    13501 cgtccgggtg acgccgggcg ctacatgccg gacggcaagt ccattgagag gccgcgcgat
    13561 cttacgctca gcggcgacgg cgaccacgaa ggagatacca gctacattgc cgtcgtggac
    13621 gacgagcgca atgccgtgag cttcacgcca agcctgcaca gcggctacgg aacaaaagtg
    13681 gcgatcggca gcctgggctt ttttctgaac tgccgcgggg actatttctc cctggtgccc
    13741 gggcacgcga atgcgctcga gccgggcaag aggccgcgca gtacactgca aagcaccatg
    13801 gtgttgaaag acggcaagct gtttttactg gcgggcagtc ccggcggcga tgaccagtgc
    13861 gcccggacca tgcagacgtt tctcaatatc gtggaattcg gaatgaatcc ccaacgcgcg
    13921 attgaagcac cgcgctggtc tacacgcgcc tttccgagct ccccctttcc acacaccatg
    13981 cgccccggcg atgcgcaggt ggagagccgg attccgcagc ccgtaagggc ggaactggtg
    14041 aggcgcggac acaaactgtt cgtccagagg cccctcacca tcggttcgaa tgcggcgatc
    14101 ctggtggatt gggagagcgg tgtgcttagc gcgggcgcgg acccccgcgc gcatgccgct
    14161 gccctggcat ggtgaggcgc gcggcacacc aaacgcgata tagtttttca aaatgctggg
    14221 acgtgtcgat ttatccaaag acctgatcct ccgctgggac catccggaac ccacgcatgt
    14281 ggatcttctg aaaaaagcac gagtggcggc ggtcgtgtgc gcggcaggca atcaggcgtt
    14341 ctccgatgcc tgctcgagcg caggcattca gacgatcca
//
LOCUS       DASTMK010000558         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_541762, whole genome shotgun sequence.
ACCESSION   DASTMK010000558 DASTMK010000000
VERSION     DASTMK010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541762"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VFQ79_23060"
     CDS             <1..1550
                     /locus_tag="VFQ79_23060"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEU0142621.1"
                     /translation="ASATMPVEGERPTELVVNPQNYHSVSPYELLNAAAQGYISIDHR
                     VLHAILDRPEEAVPDLTRFVAEDRPEDRLDLEEDLIAMFRHLRTPGAVPFFVECLRQN
                     PHDAADELTEAFVQIGAPALEPLLALYEELEEEESGEVAFILAALRVRDERVLKILLD
                     RLEFDAADGAFLLGLYGDPAAKPAIEKLLAELDPEDKAAADLRRDLEYALEHFSAPPD
                     DSFSEPFDIWSIYPEKAEPVLDILPELERLEFLKSDVEEYRSAAASSFRNELSSEAKR
                     ALFEVARNDSSAEVRGRAWEALSGETEDRAVKTAMMSRLKDEAAPLAERCGALVGLAA
                     EFDDAVVRGKMIEFYNKPEARAKALEAMWKSLDRSFAKYFPGHLEDEDIDIKRQAIWG
                     SGYLCIGSQAAELRKLFEDDEFRADALFAYALSVPAEVSRSRMKSLFRKIDEVAGGLT
                     HGEAQLVQTALDERLAFHGLDPVFNEETGEEQPDTPEGRSQVGRNDPCPCGSGKKYKK
                     CCGNPAA"
     gene            complement(1561..2244)
                     /locus_tag="VFQ79_23065"
     CDS             complement(1561..2244)
                     /locus_tag="VFQ79_23065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142622.1"
                     /translation="MLRDLIPLLLALAAAAAIYAIREWHRRRRQPNPKRPGITLAETW
                     ELWKAGVRNEADTQFVRANEFPARVAAAAAQSLERTARFFAEAGGHERDHIRRAALES
                     AATSLYLEQLTVYPERDRSALIRGYNEGMEDLLRDSALLAELRWQVLRLHLKLKYDDA
                     VPNDWFHQFLHVARPYIAEKVRLAREFVLEMNEGAGRFVEIYDELLNDLGKSALKQPP
                     KKRFVPPAR"
     gene            complement(2263..3621)
                     /locus_tag="VFQ79_23070"
     CDS             complement(2263..3621)
                     /locus_tag="VFQ79_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEU0142623.1"
                     /translation="MTGKHLHRRTFLRGLGTAVALPFLDSMMPAFGASRAAAKSTPLR
                     MAFVYVPNGVHMADWTPAREGADFELPFILEPVASFRRDFLLISGLTHNGGRALGDGP
                     GDHARAAASFLTGVHPRKTAGADICNGISADQVAAQAIGSRTMFASLELGCEDGRQAG
                     NCDSGYSCAYSNSISWRTPSNPLPPEINPRLVFERLFAGYDGESPAARAKRVLYKKSI
                     LDFVAGDTEQLKAQLGPTDRRKLDEYLFGIRDIEKRIEAAEQSAASQGSMPEQLPGKP
                     AGVPVDYAEHSRLMFDLMAVAFQTDLTRIITFMMAREGSGRSYREIDISDAHHPLTHH
                     RNNPEMIDKVRRINRYHVEQFAWFLNRLKSTPDGDGTLLNHSMIVYGSGLSDGNRHRH
                     EDLPVLVAGRGSGTLHPGRHVRFPIETPMANLYMSILERAGVEPESIGDSNGKLQELS
                     EL"
     gene            complement(3618..>5045)
                     /locus_tag="VFQ79_23075"
     CDS             complement(3618..>5045)
                     /locus_tag="VFQ79_23075"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEU0142624.1"
                     /translation="LNDDYNPEEYTLQPTDRHLTVDYIEVRGPYNIRGTLTESQQKVF
                     VCGHVTDRHHPDCVRDNLVDLARRAFRRPVSKEEAGTLVRFVKLAEEQGDTLEDGMRL
                     ALQAILVSPQFLFRIERDPPGGVAHPVTDFELASRLSYFLWSSMPDEELFRLAQAGVL
                     KNPEVLAIQTRRMLRDPRSNALAENFGGQWLQTRNLESHRPDPGKFPNFDDELRDAMH
                     RETELFFESIVREDRSIFDFLAADYTYLNDRLAKHYGIGGIEGNHFRRVDVSGASRGG
                     VLTQASILTVSSYPNRTSPVLRGKWLLENILNAPPPPPPPDVPNLDEKAVGVSGSLRQ
                     QLEQHRANPACAVCHTKMDALGFALENFDAVGAWRTHDGSFQIDANGTLPGGKAFNGP
                     GELKKILVEDPDPFVQGLVEKMLTYAIGRGLERSDRPAVQAICRKMTEQQYRFSSLVL
                     EIVNSPPFRMRRGEGERQRLRGKSG"
BASE COUNT          994 a         1493 c         1553 g         1005 t
ORIGIN      
        1 tggccagtgc tacgatgcca gtggagggag agcgacctac tgaattggtg gtcaatccgc
       61 agaattatca ttccgtttca ccctacgagc ttctgaatgc cgcagcccag ggatacatca
      121 gcatcgacca tcgcgtcctg cacgcgattc tcgaccggcc ggaagaagcc gttccggatc
      181 tcacccggtt cgtcgccgaa gaccggccgg aggaccggct cgatctcgaa gaagatctga
      241 ttgccatgtt ccggcacttg cgcacgccgg gagcggtgcc tttctttgtc gaatgcctgc
      301 gccagaatcc tcacgacgcg gcggacgagc tgaccgaggc gttcgtacag atcggcgcac
      361 cggcgctgga gcccctgctc gcgctttacg aagagttgga agaggaggag agcggggaag
      421 tcgcatttat actggctgcc ctgcgcgtca gggacgaacg cgtactgaag attcttctcg
      481 accgtctgga attcgatgcc gccgatggtg cattcctgct gggcctctac ggcgaccccg
      541 cggcaaagcc tgccatcgag aaactgctgg cggagttgga tcccgaagac aaagccgcgg
      601 cggatctccg gcgggatttg gaatacgcgc tcgaacattt ctcggcgccg ccggacgact
      661 ctttttcgga gcctttcgat atctggtcca tctatcccga aaaggccgag ccggtgctcg
      721 acatcctgcc ggagctcgaa cggctcgagt tcctcaagag cgatgtggaa gagtaccgga
      781 gcgccgcggc ttcgagcttc cggaacgagt tgtcgagcga agctaagcga gccctgtttg
      841 aagttgccag gaacgattct tcggcggaag tgcggggccg ggcatgggaa gcgctgtcgg
      901 gcgagacgga agaccgtgcg gtgaaaactg caatgatgtc gcggctgaag gacgaagcgg
      961 cgcctcttgc cgagcgctgc ggcgccctgg tggggctggc cgcggaattc gacgatgcgg
     1021 tggttcgcgg caagatgatc gagttctaca acaagccgga agcccgcgcg aaagcgctcg
     1081 aagccatgtg gaagtcgctc gaccggagct tcgcgaaata tttcccaggg cacctcgaag
     1141 acgaagacat cgatatcaag cggcaggcga tctggggctc gggctacttg tgcatcggat
     1201 cgcaggcggc cgagctgcga aagcttttcg aagacgacga gtttcgcgcc gacgcgctat
     1261 tcgcctacgc cctttccgtg ccggcggagg tttcccgcag ccggatgaaa tcgctgttcc
     1321 gcaagatcga cgaggtcgcg ggcggactca cgcacggtga agcgcaactg gtgcagaccg
     1381 cgctcgacga gcggctggcg tttcacgggc tcgatccggt attcaacgag gaaaccggcg
     1441 aagagcagcc tgacactccc gagggcaggt cgcaggtcgg ccgcaatgat ccgtgtccct
     1501 gcggcagcgg aaagaaatac aagaaatgct gcggcaaccc cgctgcctga agagcataat
     1561 ttaccgtgcc ggcggaacga accttttttt cggtggttgc tttagcgccg actttccaag
     1621 atcgttcagc agctcatcgt aaatttccac gaaacgcccc gcgccttcgt tcatctccag
     1681 cacaaattcc cgtgccaggc gtactttttc cgcaatatac ggccttgcca cgtgaagaaa
     1741 ctgatggaac cagtcattcg gcaccgcatc gtcgtacttg agtttcaggt ggagccgcag
     1801 tacctgccat cgcaattcgg cgaggagcgc cgagtcgcgc agcaaatcct ccataccctc
     1861 gttgtagccg cggatgagag cgcttcggtc cctttccggg tataccgtca gttgttccag
     1921 atacaggctc gtcgcagcgc tttcgagggc ggcgcggcgg atgtggtcgc gctcgtgccc
     1981 gccggcttca gcgaaaaacc gggctgtccg ttcgaggctc tgcgcggcag ccgcggccac
     2041 gcgggcaggg aattcattgg cacgtacgaa ctgcgtgtct gcttcattgc ggaccccggc
     2101 tttccacaac tcccacgttt cggcaagtgt gatgccgggc cgcttcgggt taggttgcct
     2161 tctacgtctg tgccattcgc gtatagcata gatcgccgcg gctgccgcca gggcaagcag
     2221 taatggaata agatctcgaa gcactcttgg atggcaagag ctttagagtt ctgaaagttc
     2281 ctggagttta ccgttgctgt ctccaatgct ctcaggctcg acccctgctc tttccaggat
     2341 agacatatag aggttggcca tgggcgtctc gatgggaaat cggacgtgcc ggccggggtg
     2401 gagtgtgccg ctgccccgcc cggcaaccag cacgggcaga tcctcgtgcc gatgccggtt
     2461 tccgtcgctg agcccgctgc cgtaaacgat catcgagtga ttgagcagag tcccatcgcc
     2521 gtcgggggtg gatttcagcc tgttcaggaa ccaggcaaac tgctccacgt ggtagcggtt
     2581 gatgcgccgc accttgtcga tcatttccgg gttgttgcga tgatgcgtca gcggatgatg
     2641 agcatcggaa atatcgattt cccggtagct gcggccgctg ccttcgcgcg ccatcatgaa
     2701 ggtgatgatc cgtgtaaggt ccgtctggaa tgccaccgcc atcaggtcga acatcaggcg
     2761 cgagtgctcg gcataatcca cgggaacgcc ggcgggtttg ccgggaagct gctccggcat
     2821 cgacccttgt gacgcggcgg actgttcggc ggcctcgatt cgcttttcga tatcgcgaat
     2881 gccgaagaga tattcgtcga gcttgcgccg gtcggtggga ccaagctgtg ccttcaactg
     2941 ttcggtgtcg ccggcaacga agtcgaggat gctcttcttg tacaaaacgc gcttggcgcg
     3001 cgcggccggg ctttctccgt cgtaaccggc gaacaagcgt tcgaaaacga gacggggatt
     3061 aatctccggc ggaagcgggt ttgacggtgt gcgccaggag atgctgttgc tgtacgcgca
     3121 actgtagccc gaatcgcaat tccccgcttg tctgccgtcc tcacagccga gttcgagcga
     3181 agcgaacatc gtgcggctgc cgatcgcttg cgcggccacc tggtccgccg agatgccatt
     3241 gcaaatgtcg gcacctgctg tcttgcgtgg gtgaactccg gtcagaaaac tggccgccgc
     3301 acgggcatga tcgccggggc cgtcaccgag cgcgcgtccg ccgttgtgcg tgagacccga
     3361 gatgagcagg aagtcacgtc gaaaagaagc aaccggttcc aatataaacg gcagctcgaa
     3421 atccgctcct tcgcgagccg gggtccagtc ggccatgtgg acgccgttcg gcacgtacac
     3481 gaacgccatc cgcaggggag tggactttgc ggcagccctg gaagcgccga atgccggcat
     3541 catcgaatcc agaaacggaa gagcaaccgc cgttcccaat ccacgcaaga aagtacgcct
     3601 gtgaaggtgc ttcccagtca tccgcttttc cctctgagtc tctgccgttc gccttcccca
     3661 cggcgcatcc ggaaaggcgg gctatttacg atctccagca ccaggctgga aaaccggtac
     3721 tgctgctccg tcattttacg acaaattgcc tgcacggcag gccggtccga acgctccagc
     3781 ccgcgtccaa tagcataggt caacatcttt tccaccagac cctgaacgaa cggatccggg
     3841 tcttccacta gaattttctt gagctcgcca ggtccgttga atgctttgcc gccgggcagg
     3901 gtgccattgg cgtcgatctg gaagcttcca tcgtgagtac gccatgcgcc cacggcgtcg
     3961 aagttttcga gagcaaaacc aagcgcgtcc atcttcgtat ggcagacggc gcaagccggg
     4021 tttgcgcgat gctgttcgag ttgctggcgc aggctgcccg acacgccgac cgctttctcg
     4081 tccaggttcg gcacgtctgg cggaggggga ggcggcggag cgttcaggat attttccagc
     4141 agccacttgc cacgcaaaac aggagatgtc cggttcggat acgaagagac ggtgagaatg
     4201 ctggcttgcg tcaggacgcc gccgcgactc gcacccgaga catccacgcg gcggaaatga
     4261 ttgccttcga tgccgccgat cccataatgc tttgcaagcc tgtcattgag gtaagtgtaa
     4321 tccgcggcga gaaaatcgaa gatgcttcga tcctcgcgaa caatgctctc gaaaaacagt
     4381 tccgtttccc ggtgcatggc atcgcgcaat tcgtcgtcga agttcgggaa cttcccggga
     4441 tcgggccgat ggctttccag gttgcgagtc tgaagccact gcccgccgaa gttttcggcc
     4501 aacgcattcg atcgaggatc gcgcaacatg cggcgggtct gtatcgccag cacttccggg
     4561 ttcttcagca cgcccgcctg cgcgagccgg aacagttctt catccggcat gctgctccac
     4621 aggaagtaag acagacgcga agcaagctca aaatcggtga cagggtgcgc gacgccgcct
     4681 ggcgggtcgc gctcgatgcg aaacagaaac tggggcgaga ccagaatcgc ctgcagggca
     4741 aggcgcatcc catcttcgag cgtgtctccc tgttcctctg cgagtttgac gaagcgaaca
     4801 agagtgccgg cctcctcttt cgaaaccggg cgccggaagg cccgccgggc cagatccacg
     4861 aggttatccc gcacgcagtc cggatgatgg cggtccgtga catgcccgca gacaaacact
     4921 ttctgctgcg attcggtcag ggtgccgcgg atattgtaag gcccacggac ttcaatgtag
     4981 tcgacggtaa ggtggcgatc cgtgggctgc aacgtatatt cttccgggtt gtaatcatcg
     5041 ttcag
//
LOCUS       DASTMK010000559         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_220257, whole genome shotgun sequence.
ACCESSION   DASTMK010000559 DASTMK010000000
VERSION     DASTMK010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220257"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3280
                     /locus_tag="VFQ79_23080"
     CDS             <1..3280
                     /locus_tag="VFQ79_23080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0142625.1"
                     /translation="LSIIAVGLVVCAGLTPVAPSQVTTASVIGYVTDTDDRTIPGAIV
                     TIKEVRTGFTRTTTTDEQGRYSIVAIPAGEYNFTVRKEGFEAIERPGQLITQQIAARI
                     DIVLKVGSVSQSVTVEASAPLLETESPANATTLSNRVITELPMIGHNYLQAAILSPGV
                     TPVAGDSMLTVTVGNYFSGGAGFKPVSVSVAGGRPDFTAFIHDGFDVRDPGYGGDLFQ
                     PSPEAISSTRIVRGYDSAQFGGEPSIVYVSTKSGTNDYHGSLWEYHQNAGLKAREFNA
                     ADVPPLTYNQAGFTFGGPALPQLRNRTFVFGEFQLTRMRSGQPNFYVVPTAEQWAGDL
                     SSIPVQLYNPFDVNPATQQRRPFPNNRIPSSLFSTIAQNYKKYVPLPNVPGAEYGAYN
                     YTRSARAITDDTQYLIRVDQILPRGGMMFAKYFNDHVTSKSEGMIPLEGFGTPLKGQT
                     ASLEWDQPLGPNKLNTLRLGFYRSHVLFGGIPTDTDITGELGFRNYDSTTAFWGFPGL
                     SVTGFSLPNTAIYDYLWITTRIGLHENFSFIKGRHSLDFGATFQPSQYPQKNGIYPRG
                     SLTYEGEFTKQSPNSAETPIGFADFLLGTFSTAWVNPDGLQPVLSSNYYSWYVQDKIR
                     VNNKLTVTLGLRWDWWSPPVERYNRWMTFDQNQGKLAYVLKDPFNWQTDFRQLREEYP
                     RGMFLNWKKKNYSPRVGIAYLLTPNTTIRVGGGLYYAQGLKNFQDFASFGSGNPPFSN
                     SLTLTNDPTRLTPGRLDNTLYDRPTIGAISPGASLVVPDIYAPQPYVEQVTFSVERQL
                     GSDMLVSAGYNGAWGRHLVNGGTNINQAALLDPDNPLPLEQRLPYPEFGFIFLQSNNS
                     NSSYNGMHLSFQKRHRHGLELTASYTWSKSIDVYTSSSSGGNNQDARCLHCDRALSDN
                     DRRHYFSLGYVWDLPFGPGRAFANAGIGSHILGNWQLSGITQLRAGTPLTPRTSTSWI
                     NVGSWIALPRSNRVCDGRKSSPTMDQYFDAECFPAQPPNTFGNSGRNVIIGPGSQLWD
                     MSLARVFAIWERLQLSLRGEVYSVFNHQNWGSPNTDVFSPTLGKIFGKSSPRTIQLGA
                     KLEF"
     gene            3311..>4619
                     /locus_tag="VFQ79_23085"
     CDS             3311..>4619
                     /locus_tag="VFQ79_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142626.1"
                     /translation="MNRRGFFRRFSLSSLLPLPWGGGVAAAGGEGPPGGGFHSQAFAA
                     LPVEESYAFHKALSEGGWHMRHDPAAKPSATEMVVAGEGWKLLLKSDAGEPLRQAAAD
                     LRAYLEIAMNTRIAVETVPNLADWRGRKKVIVAATRLDLPGCGTSLTASKDYQMKVNP
                     EQVVVCGYDELGAMYGLYNLEERLDLREGPFLPRDLNTTRRSLYKARMTMSGLGWMEW
                     PDKYLAWLPRYGFDSIFTSMCSNPNGAMTPSYLDRVPWGCSFRFRKQDPARLRDTIRR
                     AGRYGISVYCPIMYKYTGESDNEKELRKLVVDIVTQFPEIRGYVLLTEGFFYRTWFGA
                     GGHGDSDLREWIRHWARGVGIVAEECHKLNPAIEILPWDYNIDFRPNQVELKKYVIDQ
                     LPQGTIPLVTFENGKAIAFDGESGYVRDYSISVVGPSEVAAAQI"
BASE COUNT          966 a         1374 c         1342 g          937 t
ORIGIN      
        1 gctgtccatt atagccgttg ggcttgtggt ttgcgccggc ctcacacccg tcgcaccgtc
       61 gcaggtgacg acggcatccg tgattggcta cgtcaccgac actgacgacc gcacgatacc
      121 gggagcgatc gtgactatca aagaggtccg caccggcttc actcgcacta ccacaacgga
      181 cgagcagggg cgctactcga ttgtggccat cccggcaggc gagtacaact tcacggtgcg
      241 gaaggaaggc ttcgaagcca ttgagagacc aggacaactt atcactcagc agattgccgc
      301 gagaatcgat atcgttctga aagtcggctc ggtcagccag tcggtcacgg tggaggcttc
      361 agcgccgctt ctagaaacgg aatcgcccgc caacgccact acactcagca accgcgtcat
      421 cactgagctt ccaatgatcg ggcacaatta cttgcaggca gcaatcttgt caccgggggt
      481 cactccggtg gcgggtgatt cgatgctaac agtgacagtg ggaaactact ttagcggcgg
      541 cgcgggattc aagccggtca gtgtgtcggt ggcgggggga cggccagatt tcactgcctt
      601 catacatgac ggttttgacg ttcgggaccc cggctacggc ggagacctct tccagccctc
      661 tcccgaggct atcagcagca cccggatcgt ccggggctac gacagcgcac agttcggtgg
      721 cgagcctagc atcgtttacg tcagcaccaa gagcggaacg aacgattacc atggctcgct
      781 atgggaatat caccagaacg ccgggttgaa ggcccgcgag ttcaacgcgg cggacgttcc
      841 cccattgacc tacaaccaag cggggttcac ctttggcggt ccggcgctgc cgcagttgag
      901 aaacagaacc ttcgtgtttg gggaatttca gctcacgcgt atgcgctcgg gccagccgaa
      961 cttttacgtg gtgccaactg cggaacagtg ggcgggcgac ctttcgagca ttccggtaca
     1021 gctttataac ccgtttgacg taaatccggc gactcaacag cggcggccgt ttccgaacaa
     1081 ccggattccc tccagccttt tcagcacgat cgcgcagaac tacaaaaaat acgtgccttt
     1141 acctaacgtg ccgggcgcgg agtatggcgc ctacaattac accaggagcg cgcgagcgat
     1201 cactgacgac acgcagtact tgattcgcgt cgaccagata ttgcctcgcg gcggcatgat
     1261 gttcgccaag tacttcaacg accacgtcac atcgaaatcc gaagggatga ttccactcga
     1321 aggcttcggc acgcctctga aagggcaaac ggcgtctttg gagtgggacc agcccttggg
     1381 gccaaacaag ttgaacacgc tgcgcctcgg gttctaccgg tcacacgtgc tgttcggcgg
     1441 catccccacc gacactgata ttaccggcga actgggcttc aggaattatg attcgaccac
     1501 tgccttctgg ggcttccccg gcctctctgt cactggattt tcgcttccca acaccgcgat
     1561 atacgattac ctctggataa cgacgcggat cggtttgcac gaaaactttt ctttcataaa
     1621 aggaaggcac tcgttggatt ttggggcgac attccagccg tcgcagtatc cccaaaagaa
     1681 tggcatatat ccgcgcggca gcctaaccta cgaaggagaa ttcacaaagc agtcgcccaa
     1741 cagtgcggag acgccgatcg ggttcgccga ctttctgctg gggactttct ctaccgcctg
     1801 ggtaaatccc gacggtttgc aacccgtgct ttccagcaac tattactcgt ggtacgttca
     1861 ggataagatc cgcgtcaata ataagctcac cgtcaccttg ggcctgcgtt gggactggtg
     1921 gtcacctccg gtggagaggt ataaccgctg gatgaccttc gaccagaacc agggcaagct
     1981 cgcctatgtt ctgaaggatc ctttcaactg gcaaaccgat ttccggcagc tgcgcgaaga
     2041 gtatccgcgc ggaatgttcc tgaactggaa gaaaaagaac tattcgccgc gcgttggaat
     2101 agcgtacttg ctgacgccga acactacgat tcgcgtgggc ggcgggttgt actacgccca
     2161 gggcttgaag aatttccagg acttcgccag cttcggcagt ggcaaccctc ctttctcgaa
     2221 cagtctgact ttgacgaatg accccacccg gcttacaccc ggccggttgg acaacacgct
     2281 ttacgatcgt cctacgatcg gcgcgatcag cccaggtgcc tccctggtcg tgccggacat
     2341 ctacgcgcct cagccctacg tggagcaagt gacattttcc gtggagcggc aactcgggag
     2401 cgacatgttg gtgagcgccg gctacaacgg cgcctgggga cgccacctgg tgaacggagg
     2461 taccaatatc aaccaggcgg cgcttctcga tccggataac ccactgccgc tggagcaacg
     2521 gctcccctat cccgagttcg ggtttatctt cctccaatca aacaacagca acagctccta
     2581 caacgggatg cacctgagct ttcagaagcg ccaccgccac gggcttgagc tgacagcaag
     2641 ttacacttgg agcaagtcga ttgacgtgta cacgtcgtcg tcctcgggcg gcaacaacca
     2701 ggatgcgcgc tgtctgcatt gcgaccgggc cttgtcggat aatgaccggc gccactactt
     2761 ctccctcggg tacgtctggg accttccttt tgggccgggc cgcgcatttg ccaacgccgg
     2821 cataggcagc cacattttgg ggaactggca actcagcgga atcacgcagc tccgggcagg
     2881 tacgccgctg actccaagga cgtccacatc ttggatcaac gtgggctcct ggattgcgct
     2941 gccgcgatcc aaccgggttt gtgacggccg gaaaagctcg cctacgatgg accagtactt
     3001 cgacgccgag tgttttcccg cacagccgcc gaacactttt gggaattccg ggcggaacgt
     3061 gatcatcggg ccgggttcgc agctctggga tatgtcactg gcacgcgtgt ttgcgatctg
     3121 ggagcgcctt cagctcagcc tgcgcggcga agtctacagc gtgttcaacc accagaattg
     3181 gggctctccg aacaccgacg tctttagccc gacattgggt aagattttcg gcaagtcgag
     3241 cccgagaaca atacagcttg gcgccaagct ggagttttag cgcccgtcac cgccggatcg
     3301 gagggaatgc gtgaaccgcc gaggtttctt tcgaaggttt tctctgtctt cgcttctgcc
     3361 gcttccatgg ggcggaggcg tcgccgccgc aggtggggaa ggccctcccg gcggtggctt
     3421 ccattcgcaa gcgtttgcgg cgctgccggt ggaggagtcc tatgcttttc ataaggctct
     3481 ctccgaaggc ggatggcata tgcgccatga tccggcagct aaaccgtcgg cgacggaaat
     3541 ggtagtggcg ggcgaagggt ggaagttact cctcaagtcc gacgccggcg agccgttgcg
     3601 gcaggccgcc gccgatcttc gcgcatacct ggaaatcgct atgaataccc ggattgccgt
     3661 cgagacggtt ccgaacctcg ccgactggcg cggccgcaag aaggttatcg tggccgccac
     3721 gcgcctggac ctcccaggtt gtggaacgtc cctgactgcg agcaaggatt accagatgaa
     3781 agtgaaccct gaacaagtcg tcgtgtgtgg ctacgacgaa ctgggggcga tgtacggcct
     3841 gtacaatcta gaagagcgcc tggatctgcg cgaaggccct tttctgccgc gcgatctgaa
     3901 caccactcgc cgcagcttgt ataaggcccg gatgacgatg tcgggtctcg gttggatgga
     3961 gtggccggac aagtacctgg cgtggcttcc tcggtacggc ttcgactcga ttttcacctc
     4021 aatgtgctcc aatcctaacg gagccatgac accctcgtat ctggaccgcg tgccgtgggg
     4081 ctgctctttc cggttccgca agcaggaccc cgctcggctt cgcgacacaa ttcgccgggc
     4141 gggccggtac ggcatcagcg tttactgccc gatcatgtac aaatacacgg gcgagtccga
     4201 caacgagaag gagctgcgaa aacttgtagt tgatatcgtc acgcaattcc cggagatccg
     4261 tggatacgtg ctgttgacgg aagggttttt ctaccggaca tggttcggcg ccggaggaca
     4321 cggggactcg gacctccgcg aatggatcag acactgggcg cgtggcgtcg ggattgtcgc
     4381 cgaagagtgt cacaagctca acccggcgat cgagatcctg ccatgggact acaacattga
     4441 cttccgtccg aaccaggtcg agctgaagaa atacgtcatc gatcaactcc cccagggaac
     4501 gatcccgctg gtgaccttcg aaaacggcaa agcgatcgcc ttcgacggtg aaagcggcta
     4561 cgtgcgcgac tattccatca gcgtagttgg cccgtccgag gtggccgcgg cacagatca
//
LOCUS       DASTMK010000560        11933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_317302, whole genome shotgun sequence.
ACCESSION   DASTMK010000560 DASTMK010000000
VERSION     DASTMK010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11933
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317302"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..3302
                     /locus_tag="VFQ79_23090"
     CDS             159..3302
                     /locus_tag="VFQ79_23090"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142627.1"
                     /translation="MLPRYLLRLLLFVVFVFNTEAAHLSGVVYDGRGTPLFGAEVQVQ
                     SESSGARWKLLTDEQGRYSLPAVPPGPYRVRVRMPGFHTVSRSAALPSADKDLQLDFA
                     LEMIVLREVITVVSGRDDLDPSGSGALILARENTGSGLPANGRDFRAAFDLMPGVVIT
                     PAGVNDAGQFSSNGQRPNSGTFRVDGASANTGVGGNALPGAFPGASLPAMTAFGTTEN
                     LVTPENTQSVEFRPLSFAPEAGGRPGPQTIITTRSGSNQFHGSFFGRLRDHGWNARDW
                     FYNSETIAYANVFYDLPQYRATGGTFGGPIFPNRTYFFFSFDRSSLADSSYQRTSVPS
                     MKLREEAPPELGGILTFFPQPTGRELGGGESEGIIPITTNGSLKTYSLRADHSYGSHA
                     HLSGRFVQSQSSLLGSYGGGTSNWRSLTIGLTTGFSGQIHDLRFSYSNATAVGFHGGA
                     RTPLFGLSGLMPGFRIERYGPDSWGWVQDALAEPNLAKFLPPVPSVTVLGISVRGLGQ
                     FIEGYAGQARQQQWEIAHSVSRQFGQHQIRGGVQVVRLDPARDVAVNSVRGAASGLDS
                     ILDSSPIAVTLSQVPRYAGRVYAVSAFLQDTVKLSDSLNLLYGLRWELTPPTSGQQPV
                     PTISGMWTPSGWQTAYEGDVNRNAPWPMRFGQVAPRAGLAYRVPGASRLVFRAGAGLF
                     FDTALGASVNPINGAPFNSWQLSSSGTGIGTSAPALPDWLPTADSLEIRQFLSGPYPV
                     LRLPVSWQWQTSLEREAGAKGVASLAYTGSMGRNLLGNHAYVEPDTGVLKRIVTLTRD
                     ASSYHALQLRYSGMVTSALQASASYTWSHSIDNSSQDSNVFLVRPGYSTDAARGSSSF
                     DVRHALTAAMSYQTPARMPKPLRSWLVSGILRARTGFPIDIRNSEPAFGLSFINAGRP
                     DRLPGAPVWIGDSAVASGRRLNPAAFHMPAAGQEGSLGRNAITGNGLAQIDMSIRRHF
                     HLFRGMALQAEINVFNVANHPAFADPVPFLSSHWFGQSTSMQNLMLGSGRPNSGLPSV
                     FLPGGSRSVELGFRFSF"
     gene            complement(3327..4451)
                     /locus_tag="VFQ79_23095"
     CDS             complement(3327..4451)
                     /locus_tag="VFQ79_23095"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0142628.1"
                     /translation="MLPRRNFLAAFLGFAARADASPLKVERVEYIKVVVPMRPGVVVS
                     EDYGPNLDVRLRDFDKYPKFILKVHSGGLTGIGETGRDVPQEAVAKNAEFLTGKNLWQ
                     LDMASPALGLPERRTADGFEIAIYDLIGKSQGVPVYNLLGGRQQEKVAVSYWTGQRTL
                     PDLIRVGERAVELGFRNLKFKARYGDPIDRQLAAVSKAAPELSFIVDFNSSYPDLASF
                     LPVGQRLQQFNLTIEDPVPKRLDWFREFRRRLPIPLAITPSGAAQMYEAIRAEACDVF
                     NLGGTMRDFVRNCYIAELAGIPVWHGSGVELGIRDMSFIHAAAATKSCTVPSDTLCFL
                     RQSDLLATPFQVRGGYITVPTTPGLGVELDEAALRKYRVA"
     gene            4541..5848
                     /locus_tag="VFQ79_23100"
     CDS             4541..5848
                     /locus_tag="VFQ79_23100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0142629.1"
                     /translation="MGQQTSNIRWIICSFLLAISTHAFITRMSMSVAAQPIQKQFGLT
                     NVEIGWVLSAFVVGYGIVQFPVGVLTDRVGPHRLLALTLFSWSALHFLTGFSAWFAAG
                     TSILLPLIAIRFLMGIAQATVLPCCIKAIARWMPLAERATANGFFMMGLGAGGAMSPP
                     LTVALIQGFDWRAPFFLLGILGLALAGAWLSFGRDAPQLHRRVSAAELALISSGNATG
                     AHTVREPTPWRNLLANRSIWCLAFSYGVAGYPSYVFFTWFFLYVVNVHKLDIRAGGYW
                     AALPFVMIAVMTPLGGRASDLLTVRLGKRKGRLGVVLMGAFLASLLILVGARVQDARI
                     AVVLLSLGAGFHLFAQTPSWAATIDIAPAHSATVFGIMNTLAQFAGACAPVLTPLLAE
                     RFGWTSALDFAAGMAALAGLLWIGVRPDRVLTPSQPPVHALQR"
     gene            complement(5808..6374)
                     /locus_tag="VFQ79_23105"
     CDS             complement(5808..6374)
                     /locus_tag="VFQ79_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142630.1"
                     /translation="MLPKLGRIFAHIAVVIVVLSLAIPWHETAGHALTGVACGGTITE
                     FRLFGLQFAPEFRWVGLAGGRGHCEVEGIGSEWCRHLTNFSGSTSSFAVALAAAFLLW
                     RYKPGGLKRTALLSASFWAVDLLTAMLPSFGIRRYIWTGMSYSEPYQAAVGLGVPGPL
                     FQAVALAGVMAVLLLVIAAMRAQEAGKA"
     gene            complement(6387..7715)
                     /locus_tag="VFQ79_23110"
     CDS             complement(6387..7715)
                     /locus_tag="VFQ79_23110"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEU0142631.1"
                     /translation="MKAKLLFLLLFTCSLRAGWEAGVAAVDITPSEPIWLAGYAARTK
                     PMESVRQPIHAKALAIKDGTGATNVIVTLDLVGIRREMAESIAARAASELKIPRERIL
                     FNVSHTHSAPVVGGPGSYSEIMGPQLAAQKEIIARYTEKLSGLIFDAVAKSVAAIRPA
                     TLEFNQGFAGFAVNRRRVAHREYPAPVDHDVPVLAVRNTAGDLVAILFGYACHNTIMG
                     DYTVHGDYAGYAQHYLEERFPGAKALFVMGAGADSNPLPRRKPEHLERYGATLADAVE
                     EVLRAKMKPVAGPVRAAMDFPELAFEGPFDRARWEKELTSKVGYTAAHGRRMLAALAS
                     KEGVPKTRPYAVQAWQFGDSLTLIGLAGELTVDYALKFKQQYGADTTWIAGYSNDVFG
                     YIPSLRVWKEGGYEGGDAFLFSAFPGRFTSDVEDRITAAVARVLAKIRTP"
     gene            7819..8961
                     /locus_tag="VFQ79_23115"
     CDS             7819..8961
                     /locus_tag="VFQ79_23115"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0142632.1"
                     /translation="MTHRERMIAALEHREPDRVPMDLGTARFTGMVKVAYENLCAHLG
                     FGETGPVVDRMQQVVEMDEAVLRHLDVDARAVSQGAPDRGGDEELSEGEYKDEWGVVR
                     SKPPGCHYFELRRSPLAGKIDAGAIARYPYPDPSDTGRVRGLREKIRSLRDNTDYAII
                     YNARYNLVHQTQYLRGFEDWYCDLGGNHDLFRCLMDAVAENLLATNARILPEIGDLID
                     IVAFGDDVGLEDRPVCSPALYRQLIRPYQERFVAQIRANTGAKILYHSCGSVYRLIED
                     FIELGIDALNPVQVTARNMQPEQLKREFGGRIAFWGGVDSRRILPYGSPDDVKAEVRR
                     RFEEMGGGGGYVLAAVHNIQPDVPPENVLAMYDEGRNCTYAIHAAA"
     gene            complement(8964..>11933)
                     /locus_tag="VFQ79_23120"
     CDS             complement(8964..>11933)
                     /locus_tag="VFQ79_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0142633.1"
                     /translation="RNPVRLALLDPSVVDRGWGANNPFDMWGAATIDVGGSTSGRVDL
                     LLDGAPLMMTNKASYAPPMDAVQEFTVQQNSIDAEFGNSAGGIMSLSMSAGTNSFHGT
                     AYYFGRNPKLNAVSDAISRTPNFVRNHIWGGTLGNPIIKNKLFTFTAWEQWRTKDPRL
                     NIRTMPTELERTGDFSRSLNAVGGLRTIYDPWSTQLGADGTATRTPFPGNRIPTQRMD
                     PTAVRMMQDIWNPNSPGDDITGVNNFKESYTWLSRYWNFSNRTDYNISEKWRVFGRYS
                     QFHNLIDESHTVDTPAMPKDEGGAMYSFNVAGDAVYVLSPSTVLNFTGSYASIQDDYF
                     SESAAVTEEDLAGFWPNRWFSPYTKDLPAIYYPYLSIGDAVFGHRFFWIEHPKNASFQ
                     GKLVHSMGRHNVKTGISYRRSFGLINYPDPMGFYFGPGFTANTFQSPDTSLSGDSYAT
                     FLLGAVDSQSLAQYVAPHELTVNGYAAYVHDDFKVNQRLTLNLGLRFEYETGPVDRLD
                     RISRYLDLTDPIPEMQGANAPRLPAEVLAYAQAPPIYNGAWIFSSDEHRSMFNTSKHG
                     FMPRIGAAIRLNDKMSIRAGYARYVIPSSQTRPFQDTGIRLYGYTARSFVAPALVGVP
                     GGQLSDPFPASNPLILPEGKALGRYQNLGDPADWSQQGMKTGVRERINVSFQRQLAAQ
                     FVVDFTYFVNLGYNLPYAKDLNLFDPNLTYTYKAELDRTVANPFYQFSTPDKFPGQLR
                     NRENVSISSLLRPYPQYDRLRQTQTDGVKNRYQFFQVKLQRPFANGLSMFWAYNYNRE
                     RSYEFFNAVDQYEERFTYIDGFLPRHRMNIAGTYDLPFGKGRPYLSNAHAVVNAIFGG
                     WSSSAIFNYNSGQFVRFGGALVEGDPGIDNPTRDRYWDTTKFSVLPAYTNRTNPWQYP
                     GVTGPRFTNIDATLSKFFPIHGERVRLEFKMEAYNLTNSFMAGMPNTTVTSSLFGRST
                     TQANRGREMQYTLRLHF"
BASE COUNT         2329 a         3589 c         3549 g         2466 t
ORIGIN      
        1 ccgctacact cgagttgcac gaactattga accggctctt cgccggcagc tacacggagg
       61 aggcgggcga agaaatgctg ggcaatcttg ttttgcgcat ggagcgtttg gaaggagcca
      121 ttcgcgcgca ggaggaggcc ggacccggga aaaaatagat gctgcccagg tatctcttgc
      181 gactgttgct cttcgtcgtc ttcgtgttca atacggaagc ggcgcatctt tccggggttg
      241 tctatgacgg ccggggaacg ccgctcttcg gcgcggaggt tcaggtacaa agcgagtcca
      301 gcggcgcacg ctggaagctc ctgacggatg aacaaggccg ctattctttg ccggcggtgc
      361 caccgggtcc atatcgagtc cgcgtacgca tgcccggttt tcacacggtg tcgcgcagcg
      421 ccgcccttcc gtccgcggac aaagatcttc agctcgattt tgccctggag atgatcgttc
      481 ttcgcgaggt gattacagtc gtaagtggac gggacgacct cgatccatcc ggcagcggcg
      541 cactcattct ggcccgcgaa aacaccggga gcggactgcc ggcgaacggc agggatttcc
      601 gcgcggcgtt cgaccttatg cccggcgttg tgatcacacc ggcaggtgtg aacgatgcgg
      661 gccagttcag ctcgaacggc cagagaccga actcgggcac attccgggtg gatggagcca
      721 gtgccaatac aggagtcggc ggcaacgcgt taccaggagc ttttccaggc gcctcgcttc
      781 cggcaatgac cgcgtttggc actacggaaa acctggttac tcccgagaac acacaatccg
      841 tggagttccg gccgctcagc tttgcccccg aagcaggcgg ccggccgggg ccgcaaacga
      901 tcattaccac gcgctcgggc tcgaaccagt ttcacggttc gttctttggc cggctgcggg
      961 atcacggttg gaacgcccgc gactggttct ataacagcga aacaattgcc tacgcaaatg
     1021 ttttttacga cctcccccaa taccgtgcga caggaggcac ttttggcggc ccgatttttc
     1081 caaaccggac ctattttttc ttttccttcg accggtcctc tctggctgat tcctcctatc
     1141 aacgcacgtc cgtcccttcc atgaagctgc gtgaggaggc gcctcccgag cttggtggaa
     1201 ttctgacctt ttttccgcag cccaccgggc gcgagctggg aggcggcgaa tcggagggaa
     1261 taatccccat tactacaaac ggctctttga agacctacag tcttcgcgcc gaccactcct
     1321 acggctcaca cgcacacttg tccggcagat tcgtacaatc gcaatcgtcc ttactaggga
     1381 gttacggggg aggaacttcc aactggcgca gccttacaat cggtttgacc accgggtttt
     1441 ccggacagat ccacgacctg cgattcagct attccaatgc cacggctgtc ggcttccatg
     1501 gaggcgctcg taccccgctg ttcgggcttt ccggactgat gcccgggttt cgcatagagc
     1561 ggtatggccc cgattcctgg ggttgggtcc aggacgcctt ggctgagccc aatctggcta
     1621 aattcctgcc gccagtcccc tcggtcactg tgctcgggat ttcggtacga ggactgggcc
     1681 agtttatcga aggctacgcc ggccaggccc gccagcaaca gtgggaaatt gcccactcgg
     1741 tttcgagaca atttggacag catcagattc gagggggagt gcaagtcgtc cggctcgatc
     1801 ccgcccggga cgtagccgtc aacagtgttc ggggagcggc ttcggggctc gacagcatcc
     1861 tcgacagtag tccgatagcg gttacgctgt cgcaggtacc gcgatatgcc ggcagagtct
     1921 acgctgtgtc ggcgtttctg caggacaccg tcaagctcag cgattcgctg aatctccttt
     1981 acggccttcg ttgggaactc acgccgccta cgagcgggca gcaaccggtt cccaccatct
     2041 ccggaatgtg gacaccaagc ggctggcaga ccgcttacga gggagatgtg aatcgcaacg
     2101 ccccatggcc catgcgtttt gggcaggttg ctccgcgcgc cggtttggct tatcgtgtac
     2161 cgggcgcgag ccggcttgtc tttcgcgcgg gcgccgggct cttcttcgac acagcgctgg
     2221 gagcaagcgt caatccgatt aacggcgcgc cgttcaacag ttggcagcta tcttcctcgg
     2281 gaacaggcat cggcacatcg gctcccgctt tgcctgactg gctgccaact gcggactcac
     2341 tggagatcag gcagtttctc tcgggacctt atcccgtcct gcgtcttccc gtgtcctggc
     2401 aatggcaaac ttccctggaa cgggaagcgg gcgccaaagg ggtcgcttct cttgcctata
     2461 ccggctcgat gggaaggaat cttctaggga atcatgccta cgttgagccc gatacgggag
     2521 tcttgaagcg tatcgttacg ctgacccggg atgcttcgag ctaccacgct ctccagttgc
     2581 ggtactccgg aatggtaacc tcggctcttc aggcgtctgc atcctacacc tggtcccatt
     2641 ccatagacaa cagctcccag gactccaatg tctttttggt gcgcccgggc tacagcacgg
     2701 atgcagcccg gggttcatcg agctttgatg tccggcatgc gttgacggcg gccatgtcgt
     2761 accagacgcc ggcgcggatg cccaagcctc tgcgaagctg gctggtgagc ggcatccttc
     2821 gcgcccgcac cggtttcccg attgacatcc ggaacagcga accggcgttt gggctgagtt
     2881 tcatcaatgc cggacgcccg gatcgcttac ctggtgcgcc tgtctggatc ggggattctg
     2941 ccgtagcaag cggccgccgg ttgaatccgg ccgccttcca catgccggct gcgggtcagg
     3001 aaggaagcct gggacggaat gcgatcaccg gaaacggcct ggcgcagatc gatatgagta
     3061 tccgcaggca tttccatctc tttcgcggaa tggcgctgca agcggaaatc aatgttttca
     3121 atgtcgccaa tcatcccgcg tttgccgatc ccgtgccctt cctgtcgagt cattggttcg
     3181 ggcagtccac ttccatgcag aacctgatgc tcggttcagg aagacccaat agcggcttgc
     3241 cctccgtctt tcttccgggc ggttccagat cggtagaact cggttttcga ttctcgtttt
     3301 gagcggcgag actgttgggt cgaaggtcaa gccacccgat attttcgcag cgccgcctcg
     3361 tcgagctcaa cgcccagccc tggcgttgtc ggaaccgtga tgtagccacc tctgacctgg
     3421 aacggcgttg ccagtaggtc gctctgccgc aaaaagcaga gggtatccga tggaacggtg
     3481 caactcttcg tcgcggccgc cgcgtggatg aaggacatgt cgcgaatccc gagttctact
     3541 ccggagccgt gccacacggg gatgcccgcc agttccgcta tgtagcaatt gcgcacaaaa
     3601 tcccgcatcg taccgcccag gttgaacacg tcgcatgctt cggcgcgtat cgcttcatac
     3661 atctgggccg ccccggacgg tgtaatcgcc agtggaatgg gaagccgccg ccggaactcg
     3721 cgaaaccagt ccagccgttt aggcacaggg tcctcgatag taaggttgaa ctgttgaagc
     3781 cgctggccca ccggcagaaa actcgccaga tccgggtagg acgaattgaa gtccacaatg
     3841 aaactcagtt ccggcgctgc tttcgaaacc gcggccaact gccggtcgat gggatcgccg
     3901 tagcgcgcct tgaacttgag atttcgaaag ccaagctcaa cagccctctc gccgacgcgg
     3961 atcaggtccg gcagagtgcg ctgccccgtc cagtaggaga cggcgacctt ttcctgttgg
     4021 cggcctccca gaagattgta tacgggcacg ccctggctct tgccgatcag gtcgtaaata
     4081 gcgatctcaa aaccatccgc ggtacggcgc tccggcaggc ctaaagcagg agaagccatg
     4141 tcgagttgcc acagattctt tcctgttaaa aactccgcat tcttcgccac ggcctcttgc
     4201 ggaacatcgc ggccggtttc gccgatgccg gtcagcccgc ccgaatgcac tttgagaatg
     4261 aactttggat acttatcgaa atcgcgcaga cggacgtcca ggttgggtcc gtagtcctcg
     4321 ctgacaacta cacccggccg catcggcaca accaccttga tgtactcgac gcgttccact
     4381 ttcaaaggtg aggcatcggc ccgagcggcg aatcccagaa aggcggcaag aaaatttcgt
     4441 cgcggcaaca tatcgatctc ctccggttcc gtacactgta tcgcaggaac tcaccgtcgc
     4501 gctcgcgtat gatagtctgc acggcaccgg gcaatcgaac ttgggacaac agacctccaa
     4561 cattcgctgg atcatttgca gcttcctgct ggccatcagc acacatgcgt tcatcactcg
     4621 gatgtcgatg agcgtggcgg ctcaacccat tcagaaacag ttcggcctca ctaatgttga
     4681 gatcggttgg gtgctgagtg ccttcgtcgt tggctatggc atcgtacaat tcccggttgg
     4741 tgtgttgacc gaccgcgttg gaccgcaccg tctgctggcg ctcacgctat tctcctggtc
     4801 ggcacttcat ttcctgacgg gcttcagtgc gtggttcgcc gccggcacaa gcatcctgct
     4861 tccactgata gcgatccgat tcctgatggg catcgcgcag gcgaccgtgc tgccctgctg
     4921 cattaaagcg atcgcacggt ggatgcctct cgccgagcgc gccaccgcca acgggttttt
     4981 catgatgggg ctcggcgccg ggggagccat gagtccgccg cttaccgttg ccttgataca
     5041 aggcttcgac tggcgggcac cgttcttcct tctcggcatc ctcggtctgg cgctggccgg
     5101 cgcgtggttg tcgttcgggc gggatgcccc gcaattgcac cggcgcgtca gcgccgccga
     5161 actggcgctg atctcaagcg gcaacgcgac cggtgcacat accgtccggg aacctacgcc
     5221 gtggcgcaac ctgctcgcga atcgcagcat atggtgtctg gcgttcagct atggtgtggc
     5281 gggctatccg tcgtacgtat ttttcacgtg gttcttcctg tacgtggtga acgtacataa
     5341 gctcgacatc cgtgcaggcg gatactgggc agcgctcccc ttcgtgatga tcgcggtgat
     5401 gaccccgctg ggaggccggg cttccgacct gctgaccgtg cgcctcggta aacgcaaagg
     5461 gcggctcggc gtggttctca tgggagcatt cctcgcgtcg ttactgatac tggtgggcgc
     5521 gcgcgttcag gatgcacgca tcgcggtggt gcttctgtcg cttggcgccg ggtttcacct
     5581 cttcgcgcag actccgtcgt gggcggccac catcgatatc gcccccgcgc actcggcgac
     5641 ggtgttcggc ataatgaaca cgctggcaca attcgccggc gcatgcgccc cggtgctcac
     5701 gcctctgctg gcggagcggt tcggttggac cagcgctctg gatttcgcag ccggcatggc
     5761 cgcgctggcg ggacttctct ggatcggagt tcggccagac cgcgtgctta cgccttccca
     5821 gcctcctgtg cacgcattgc agcgatgacc agtaagagaa ccgccatcac cccggccagt
     5881 gcgacagcct ggaacagagg acccggcacg cctaatccga ccgccgcctg ataaggctca
     5941 ctgtagctca tgccggtcca gatgtagcgt ctgattccga aagagggcaa catcgccgta
     6001 agcaggtcca cggcccagaa gctggcactg agcaacgccg ttcgtttcag cccgcctggt
     6061 ttgtatcgcc acaagaggaa agccgccgcc agcgcgaccg cgaaactgct ggtggatccg
     6121 gaaaagttcg tcaggtgcct gcaccattcc gagccgattc cctcgacctc gcaatggccg
     6181 cgtccgccgg cgagcccgac ccaccggaac tccggcgcga actgaagtcc gaacaaccgg
     6241 aactccgtta tcgtgccccc acaggcaact ccggtgaggg cgtggcccgc cgtttcatgc
     6301 cacgggatgg cgaggctgag cacgacgatc acaacagcga tgtgcgcgaa aattcgcccg
     6361 agtttaggca gcattccagc gtccggtcac ggcgtgcgaa tcttcgccag aacacgcgcg
     6421 acagcagcgg tgatgcggtc ctccacatcg gaggtgaacc gccccggaaa ggccgagaac
     6481 aggaacgcat cgccgccttc gtagcctccc tccttccaga cccgcagcga tggaatgtag
     6541 ccgaagacgt cgttgctgta gccggcaatc catgtcgtat cggcaccata ctgttgctta
     6601 aacttcagcg cgtagtcgac ggtcagctcg ccagccagcc cgatgagcgt aaggctatcg
     6661 ccgaactgcc acgcctgcac ggcataaggt ctggtcttcg ggacaccctc tttactcgcg
     6721 agcgccgcca gcatcctgcg gccatgggcc gccgtatacc cgaccttgga tgtaagttcc
     6781 ttctcccaac gggcacggtc gaatgggccc tcgaaggcaa gctccgggaa atccatcgcc
     6841 gctcgcaccg gtcccgcaac cggcttcatc ttggcgcgaa ggacctcttc caccgcgtcg
     6901 gcgagcgtcg ctccgtagcg ttccagatgc tcgggctttc tacggggaag cgggttggaa
     6961 tcggcgccgg ctcccataac aaaaagcgcc ttcgcaccgg ggaaacgttc ttcgagatag
     7021 tgttgcgcgt aacccgcgta gtcgccgtga accgtgtaat cgcccataat cgtgttgtgg
     7081 caggcgtatc cgaatagaat tgcaacaaga tcaccggcgg tattgcggac cgcaagcacg
     7141 ggcacatcgt gatcgaccgg cgccgggtac tcccggtgtg cgacgcggcg gcggttcacg
     7201 gcaaagccgg caaagccctg gttgaattcg agtgtagccg gcctgatggc tgccaccgat
     7261 ttcgctacgg catcgaagat gagcccggac agtttctccg tgtagcgggc gataatttcc
     7321 ttttgcgcgg caagctgcgg tcccataatt tcggagtaac tgccgggtcc gcccacaacc
     7381 ggcgcgctat gggtgtgcga gacattaaat agaattcgct cgcgcggaat cttcaattcg
     7441 ctcgcggcac gcgccgcaat ggattcggcc atctcgcgcc ggatgccgac cagatcgagc
     7501 gtcacaatga cattcgttgc gcccgtgccg tctttgatcg ccagagcttt ggcgtgtatc
     7561 ggctggcgaa cggattccat cggtttcgta cgagccgcat agccggcaag ccagatcggc
     7621 tcggaaggag tgatgtcaac cgccgccacc ccggcttccc atccggcgcg gagcgagcaa
     7681 gtgaacaaca ggaggaatag cagtttcgct ttcatctgtc ctcaacagaa tatattcttc
     7741 cagcgcccat aaggcaataa acggagtttg ttccagacta tcagagcttg tactatcgtt
     7801 ttgggcggag gcaaaagtat gacacaccgt gaacgaatga tcgccgcact ggaacaccgc
     7861 gagccggacc gcgtaccaat ggatctcggt acagcccggt tcaccggcat ggtgaaagtg
     7921 gcctatgaga acctgtgcgc gcatctcggg ttcggagaaa ccggtcctgt ggtggaccgt
     7981 atgcagcagg tggtggagat ggacgaagcc gtgctgcgcc acctggacgt cgatgcgcgt
     8041 gccgtgtcgc aaggcgctcc cgaccgcggc ggggatgagg aactttccga gggggaatac
     8101 aaagacgagt ggggagtggt ccgctccaag ccacccggct gccactattt cgaactcagg
     8161 cgttctccgc tcgcaggcaa aatcgatgcg ggcgccatcg cgcgttaccc ctatcccgat
     8221 ccatccgaca caggccgggt gcggggctta agagaaaaga tcaggagcct gcgcgacaac
     8281 acggactacg ccatcatcta caacgcacga tataacctgg ttcatcagac acaatacctg
     8341 cgcgggttcg aagactggta ctgcgatctc ggcggcaacc acgatctctt ccgttgtctg
     8401 atggatgccg tagcggaaaa cctgttagcc accaacgcgc gcattctgcc ggaaattggc
     8461 gatctcattg acatcgtggc gttcggtgac gacgtgggcc tggaagaccg cccggtctgt
     8521 tcaccggccc tgtaccgtca gttgatccgg ccctatcagg agcggttcgt cgcacagatt
     8581 cgcgccaaca ccggggcaaa aatcctctat cactcctgcg gttcggtcta tcggcttatc
     8641 gaggatttca tcgaactcgg catcgacgcc ctgaacccgg tacaggtgac cgctcggaac
     8701 atgcagccgg aacagttgaa acgcgagttc ggcggccgca tcgcattctg gggcggcgtt
     8761 gacagccggc gtatccttcc gtacggctcc cccgacgacg tgaaggcgga agtccgccgc
     8821 cgtttcgaag agatgggtgg cggaggcggc tacgtgctgg ctgccgtgca caacattcag
     8881 cccgacgtgc cgcccgaaaa tgttctcgcc atgtatgacg aaggacgaaa ctgcacctac
     8941 gccatacacg cggccgcctg aaatcagaag tgcagccgga gcgtgtactg catctcgcgt
     9001 ccgcggtttg cctgcgtggt ggaacggccg aacagagaac tggtgacagt ggtgttgggc
     9061 attcccgcca tgaagctgtt cgtcaggttg taagcttcca tcttgaattc cagccggacc
     9121 cgctccccgt ggattgggaa gaacttcgaa agcgtcgcgt cgatattcgt aaagcgcggg
     9181 ccggttacac cgggatattg ccacggattc gtacgattcg tgtaagccgg cagaacactg
     9241 aacttcgtgg tgtcccagta acggtcccgg gtgggattgt cgatacccgg atcgccctcc
     9301 accagcgctc cgccgaaccg gacaaactgg cccgagttgt agttgaagat cgcgctcgag
     9361 gaccagccac cgaagatcgc attgacaacg gcatgtgcat tgctcagata cggacgtccc
     9421 ttgccgaaag gaagatcgta ggtacccgcg atattcatac ggtgccgcgg cagaaagccg
     9481 tcgatgtaag tgaaccgctc ctcgtattga tcgacggcat tgaaaaactc gtagctccgc
     9541 tcgcggttgt agttgtatgc ccagaacatg ctcaggccgt tggcaaacgg gcgctggagt
     9601 ttgacctgga agaactggta gcggttcttc acgccgtcgg tctgtgtctg ccgcaacctg
     9661 tcatattgcg ggtacggccg cagcagactg ctgatactta cattttcgcg attacggagc
     9721 tgcccgggaa acttatccgg cgtggagaat tgatagaaag gattggcaac ggttctgtct
     9781 aactccgcct tgtaagtgta ggtcaggttc ggatcgaata gattcaagtc cttggcataa
     9841 ggcaggttat agcctaagtt cacaaagtaa gtgaaatcga ctacgaactg tgccgctaac
     9901 tggcgctgga aagaaacatt gatgcgctcg cgaacgcccg ttttcattcc ctgctggctc
     9961 cagtcggcgg gatctcccag gttctgataa cggcccaatg ctttgccctc gggaaggatc
    10021 agcgggttgg aggccgggaa cggatcgctc aactggccgc ccggaacgcc aaccagtgcg
    10081 ggggccacga acgaacgggc agtatagcca taaaggcgaa tgcccgtgtc ctggaacggc
    10141 cgcgtctggc tggaaggaat gacgtaccgt gcatacccgg cgcggataga catcttgtcg
    10201 ttcagccgga tggccgcacc gattctcggc atgaagccat gcttcgaggt gttgaacatg
    10261 ctccgatgct catcgcttga aaagatccac gcaccgttgt agatgggcgg agcttgcgcg
    10321 taggcgagca cttccgccgg cagcctgggg gcattcgcgc cctgcatctc cggtatgggg
    10381 tcggtcagat cgaggtagcg cgagatgcgg tccaggcgat ctaccggccc ggtttcgtat
    10441 tcgaagcgca gccccagatt cagagtgagg cgctggttca ctttgaaatc atcgtgcacg
    10501 taggccgcat agccgttcac agtcaattcg tgcggagcca catactgcgc cagggactgc
    10561 gagtcgactg cgccgagaag gaacgtcgca tagctgtcgc cgctgaggct tgtatcgggg
    10621 ctttgaaaag tgttggcggt gaatcccgga ccgaaataga agcccatcgg gtccggatag
    10681 ttgatcagcc cgaaggaacg ccggtaggag atgcccgtct tcacattgtg gcgccccatg
    10741 gaatgcacca gttttccctg gaagctcgcg tttttcggat gttcgatcca gaagaagcgg
    10801 tgaccgaaga ccgcgtcgcc gatactcaga tacggatagt agatcgcggg caaatctttc
    10861 gtgtaaggtg aaaaccaccg gttgggccag aaccccgcca ggtcctcttc ggttaccgcg
    10921 gcggattccg aaaaatagtc gtcctgaatc gatgcgtagc tgccggtaaa gttcaggacg
    10981 gtgctcgggc tcagtacata tacggcgtct ccggcgacgt tgaaggagta cattgccccg
    11041 ccctcatcct tgggcatggc cggagtatcc accgtgtggc tttcgtcgat caggttgtgg
    11101 aactggctgt acctgccgaa cacgcgccac ttctcgctga tgttatagtc cgtgcggttt
    11161 gaaaaattcc agtagcgtga aagccatgtg taggactctt tgaaattgtt cacgccggtg
    11221 atatcgtcgc cggggctatt cggattccag atgtcctgca tcatacgcac agcggtcgga
    11281 tccatccgtt gtgtgggaat gcggtttccg ggaaacggcg ttcgcgtcgc ggtaccgtcc
    11341 gcaccaagtt gcgtgctcca cggatcgtag atggtacgga gtccacccac cgcatttagc
    11401 gagcgggaga aatcaccggt gcgctccaac tccgtgggca tggtgcgaat gttcaggcgc
    11461 gggtccttgg tacgccattg ctcccacgcc gtaaaggtga acagcttgtt tttgataatc
    11521 ggattgccga gtgtaccgcc ccagatatgg ttgcggacga agttaggcgt gcggctgatc
    11581 gcgtcgctca ccgcgttcag tttggggttc cggccgaaat aatacgccgt tccgtggaaa
    11641 gagttagtac ccgccgacat ggacagactc atgatgcccc ccgcgctgtt gccgaactcg
    11701 gcgtcgatgc tgttctgctg cacggtgaac tcctggacgg catccatcgg aggcgcataa
    11761 gacgctttgt tcgtcatcat gagtggagca ccatccagta gaagatccac cctgccgctg
    11821 gtgcttcctc ctacgtcaat ggttgcggcg ccccacatgt cgaagggatt gttcgcgccc
    11881 cagccgcgat ccactaccga cggatcgagc agtgccaacc ggactggatt gcg
//
LOCUS       DASTMK010000561         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_285202, whole genome shotgun sequence.
ACCESSION   DASTMK010000561 DASTMK010000000
VERSION     DASTMK010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285202"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2145
                     /locus_tag="VFQ79_23125"
     CDS             <1..2145
                     /locus_tag="VFQ79_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEU0142634.1"
                     /translation="RRQDANRMAHIYLCFLWHMHQPFYKDLLSGEYRLPWTRMHALKD
                     YYGMVHILEEFPEVHQTFNLVPSLMVQVEEYARGEAVDPFLKCALKPAEELTEHEQDF
                     ILRYFFQASVTRLINRYPRYAELYEAWQVAGRNPVRARRFFGTSAMRDLQVLSQLAWF
                     DEEFLAHDAEVSALVKKEREYTVDDQLLLGRKQTEMIGLVLPAYAEALRRSQIEVSTT
                     PFYHPILPLICDSNIAQVSHPHVPLPTRFRYPGDARWQLDHARSYMESRLGSAPVGLW
                     PSEGSVSDEALEIAADAGFRWFATDNGVLGRTLGSVAGVEQTYKSYRWKREGRELLCL
                     FRDHYLSDLIGFVYQRMEANDAAGHFLGLIRDNCRGMLASGRDALVPIILDGENAWEH
                     YPENGRPFLQELYRRISEDPDMRAVTVSEAFERIEAETIHNIFPGSWINANFDIWIGA
                     EEDNKAWEYLLRARQAYDRVVSSEPGLSIEAKRVAHEELMIAEGSDWCWWYGPQHESA
                     NRPEFDRLFRDHLANVYRALNVSPPEELSRPILQVSIREFHELPTAPVRPSIDGEVTS
                     YFEWLGAGLYKVDGRSGAMHGQRYLIRELRYGSDGYSIYLRVDFEEGFDPADMEARFT
                     IAQAAGSGASASIVLNLSANGVEPVGSSLQGEYAYHSVLEMKLPLPALGISIGNPVRV
                     QMSMWHGGLPVDALPPQGWLEVSTAEPSDWPL"
     gene            2208..>2469
                     /locus_tag="VFQ79_23130"
     CDS             2208..>2469
                     /locus_tag="VFQ79_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142635.1"
                     /translation="MVSAEEQKPRRLNKVIELLESRRQPPLGVFAFNPTPRSGASIAS
                     SAVDFVIVDMEHAPYDPARLEGYLLGMIDKRRIVSSGSLQPPV"
BASE COUNT          486 a          695 c          754 g          534 t
ORIGIN      
        1 cggagacagg acgctaaccg aatggcgcac atttacctct gttttctctg gcacatgcac
       61 cagccgttct ataaggacct gctctcaggc gagtacaggc ttccctggac acgcatgcat
      121 gcgttgaaag actactatgg aatggttcac attctggaag agttccccga agtacaccag
      181 acctttaacc tggtcccatc gctgatggta caggtggaag agtacgcccg gggcgaagca
      241 gttgatccat ttctcaagtg cgccctgaag cctgccgagg aactcacgga gcacgagcag
      301 gatttcattc tccggtattt ttttcaggcc agcgtaacac ggctgatcaa tcgttatccc
      361 cggtatgcgg aattgtacga ggcgtggcag gtggcgggca gaaaccccgt gcgagcgcgg
      421 cgctttttcg gcacttcggc gatgagggat ttgcaggtac tctctcaact cgcctggttc
      481 gacgaagaat ttctggccca tgatgccgag gtatcggcgc tggtgaaaaa ggagcgcgaa
      541 tatactgttg acgaccagtt gctgctggga cgcaagcaga cggagatgat cggcctcgtc
      601 ctgccggctt acgcggaagc gttgcgccgg agccagatcg aggtttcgac aactcctttc
      661 taccacccca ttcttccgtt gatctgcgat tccaatattg cgcaagtttc gcacccgcac
      721 gtgccgctgc ccacccgatt ccggtatccc ggcgatgcgc gctggcagct cgatcacgct
      781 cgcagctata tggaatcgcg cctgggctcg gcgcccgtgg ggctgtggcc gtcggaagga
      841 tcggtttccg acgaggcact ggaaattgcc gccgatgccg gtttccgctg gttcgccacc
      901 gacaatggtg tgctgggccg cacgctcggc agtgttgccg gagtggagca gacctacaag
      961 tcgtaccgct ggaaacgaga gggccgggaa ctgctctgcc tgttccgcga ccattatctc
     1021 agcgacctga tcgggttcgt ctaccagcgt atggaagcca acgacgccgc cgggcatttc
     1081 ctcggattga ttcgcgataa ctgccgtggg atgctcgcaa gtgggcgcga tgcgctggtg
     1141 cccatcattc tggacgggga aaacgcctgg gagcattacc cggaaaacgg ccggccgttt
     1201 ttacaggagt tgtaccgccg catcagtgag gacccggaca tgagggcggt cacggtttcc
     1261 gaagcgttcg aaagaatcga agcggaaacg atccacaaca tttttccagg ttcctggatt
     1321 aacgccaatt tcgatatttg gattggcgcg gaagaagaca acaaggcgtg ggagtatctg
     1381 ctgcgggcac ggcaggctta cgatcgcgta gtttcctccg agcccgggct gagcatcgaa
     1441 gcaaaacgtg tggcgcacga ggaactgatg attgccgaag gcagtgactg gtgctggtgg
     1501 tacggtccgc agcatgaatc ggccaaccgc cccgagttcg acaggctctt ccgggaccat
     1561 ctggcgaatg tctatcgtgc gttaaacgtg tctcctccgg aggagttatc gaggccgatt
     1621 cttcaggttt cgatccgcga attccacgag ctgcccaccg cccccgttcg tccgtctatc
     1681 gacggcgagg tcacttcgta cttcgaatgg ctgggcgccg ggttgtacaa agtggatggc
     1741 cgctccggcg cgatgcatgg acagcgatac ctgatccgcg aattgcggta cggcagcgac
     1801 ggatacagta tctacctgcg tgtagatttc gaagagggct tcgatccggc ggacatggag
     1861 gcgcggttca ccatcgcaca ggctgccggc tccggagctt ccgctagcat cgtgctgaat
     1921 cttagcgcaa atggtgttga gccggtggga tcctcattgc agggcgaata tgcctatcat
     1981 tccgtcctcg agatgaaact gcctctgccg gcgcttggaa tctcgatcgg gaatccggtg
     2041 cgcgtgcaga tgtcgatgtg gcatggagga cttcctgtag acgccttgcc tccacaaggg
     2101 tggctggaag tatcgaccgc ggagccgagc gactggccct tgtagaaagg tatcgactac
     2161 ttatgcgtgt cgttgccgct tgcctgttgg tttgtttcct ggggaagatg gtttccgcgg
     2221 aggagcaaaa gcccaggcgc ctcaacaagg ttattgaatt acttgagagc cgccgtcaac
     2281 cgccgctcgg tgtcttcgcc ttcaatccca cgccgcggag cggcgcgtca atcgccagtt
     2341 ccgcggtcga tttcgtcatc gtcgacatgg aacacgcgcc gtatgatccg gcgcgtctcg
     2401 aaggctatct gctgggtatg atcgacaagc gccggatcgt ctccagcggt tcgctgcagc
     2461 cgcccgtga
//
LOCUS       DASTMK010000562         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_348506, whole genome shotgun sequence.
ACCESSION   DASTMK010000562 DASTMK010000000
VERSION     DASTMK010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348506"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFQ79_23135"
     CDS             complement(<1..573)
                     /locus_tag="VFQ79_23135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142636.1"
                     /translation="MRTLLLITTAALLPAQLPGAGPIWDFAAVGGGSFYLSKTVTSES
                     AKASTGFRTGFAGGLILGHEMYRNIGGEIRYLFLKNDMKVSGNGESATFSAQSHAIHY
                     DLLFYTAPPESAVRPFIAIGGGGKYYRGTGTEPAYQPLSQLVLLSKADQWSGMLAAGA
                     GFKVALRSGLHLRLEFRDYMTPLPKKLLTPN"
     gene            complement(589..3615)
                     /locus_tag="VFQ79_23140"
     CDS             complement(589..3615)
                     /locus_tag="VFQ79_23140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142637.1"
                     /translation="MKTNGCWKLLMLGLLLVAAASAGTFGKVVVIGGHAADLALDEAR
                     GRLYIANFGANRIEIMSLASRSLEPASMNVAPQPASMALSPDGRYLVVTHYGNFQAPG
                     SSSNALTVIDLETNDRKTFAMGNPPLGVAFGIDGLALIVTTNEFVLFDPLLGTTQVLA
                     TVEGVTANTLPVPPPKFPPQIVAASVAASADHTMVFGLTDTIRFRYDVRMKQVISLGY
                     TATPPLGPRVVSVSRDGQIYAAGWAVFRPVGASDVLMAQFRDASGSLNTGGHAIDSER
                     SIFYGQVPRAAPVVPSDSSSSGTPVVSTTPDPPELLVADLDNLTVRERLRLPENLAGK
                     SVLSADGSTMYAASDSGVLVLPVGSLDQYRRVRAQHEDLLFQSNACDRRSQTQELVIS
                     DPGGGATPFAITSSSPGVSVSPSSGVTPLTVRVRVDAGSFQGQRGTITATLTISSPGA
                     VNIPETVRVLVNLRDPDQRGTVINVPGKLVDVATDPDRDRFFVLRQDKNQVLVYDGTN
                     YSLLHTLRTGNTPTQMTISFDRHYLIVGNDNSQIANVFDLETLEPQQPIVFPAGHYPR
                     SIAASGNAILAASRVAGPIHMIDRVDLGTRRAVELPSLGIYKNDVDADTVLTASPNGS
                     AILVAQANGTIMLYNANVDSFIASRKDETSLSGGYAASSYDVFAVGHQMLNASLYPTQ
                     KFSSATGLSSGYVFVDRGGFRTTAANSASPGVIERLDVGGPIAMRATRIAEAPVLKDV
                     VSAFTRTVAPLYSRKAIINLTTSGFTVLAWDYDASLAPPRIDSIVNAADLRQPVAPGG
                     LITLFGNNLSAINAATKEMPLPTALGETCLTANGVPMPMLFVSPGQINAQLPFQVDGN
                     ATLILRTPGGVSDNFNLTISPAAPSIFHSGIAGPNSNLPTIYRSNNSLVTISNPIHRG
                     ETITIYLTGLGKTWPAVETGMPAPADPPAASLVAPTATLGGVSLDVKFAGLAPGQVGV
                     YRIEAGVPITISTGLSIPLLISQGGSTTSVEVRVVE"
     gene            complement(3823..>4069)
                     /locus_tag="VFQ79_23145"
     CDS             complement(3823..>4069)
                     /locus_tag="VFQ79_23145"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0142638.1"
                     /translation="KLVAVLIQADRFGTGVSQSLRGHADYMRIQARQVAEEKAAKLGV
                     KLLFPIFFCVLPSLFVVTVGPMAVKIVRELIPMMNNI"
BASE COUNT          878 a         1153 c         1214 g          824 t
ORIGIN      
        1 gttcggagtc agcagttttt tgggcagcgg cgtcatatag tcgcggaatt cgaggcgcag
       61 gtgtaggccg gatcgcagcg ccaccttaaa accggctccg gcggcgagca taccgctcca
      121 ctggtctgcc ttgctcagca gcacaagttg gctcaatggc tggtaggcgg gctcggttcc
      181 agtgccccga tagtattttc ctccgccgcc aatagcgatg aagggccgga ctgcggactc
      241 gggaggcgct gtatagaaaa gcaaatcata gtgtatcgcg tgagactgcg cggaaaatgt
      301 ggccgactcg ccgtttcccg acaccttcat gtcgttttta agaaaaagat atcggatctc
      361 cccgccaatg ttgcgataca tctcatggcc caaaatcagg ccgccggcaa acccggtcct
      421 aaatcccgtg gatgccttcg cactctctga cgtaacagtc tttgagagat agaaactgcc
      481 gccgccaacg gcggcgaagt cccaaatcgg gccggctcct gggagttggg cgggcagcag
      541 ggcagcagtc gtgatgagaa gcaaagtccg catgataatc gatccgtgtc attcgacgac
      601 acgtacttca acactagtag tgcttccacc ctgggaaatt aacagaggaa ttgaaagtcc
      661 ggtggaaatt gttatcggaa cgcccgcttc gatccggtag acacccacct ggcctggagc
      721 aagaccagcg aacttcacgt cgaggctcac gccgcccaga gtcgccgtag gcgccacaag
      781 actggcggcg ggcgggtccg cgggagccgg catgccggtt tccacggcag gccatgtttt
      841 tccgagcccg gtgagataga tcgtaatcgt ttcgcctcta tggatcgggt tggaaattgt
      901 taccaggctg ttgttgctcc ggtaaatcgt gggaaggttg ctattcgggc cggctattcc
      961 gctatgaaaa atactcggag cggcaggtga aatcgtcaaa ttaaagttgt cgctgacgcc
     1021 tccgggagtt ctcaaaatca atgttgcatt tccgtccacc tggaagggaa gttgcgcatt
     1081 aatctgaccg ggcgaaacaa acagcattgg catcggtacc ccgtttgccg tgagacaggt
     1141 ttctcccagc gcagtgggaa ggggcatttc tttcgttgcg gcgttgattg cgcttagatt
     1201 attcccgaac agggtgatca gccctcccgg cgcgaccggc tgcctcaggt ccgctgcatt
     1261 gacaatgctg tcgatacgcg gaggagccag tgaagcatcg tagtcccagg cgagcaccgt
     1321 gaaaccggat gtagtcaggt ttatgatggc tttgcggctg tacagcggag ccaccgttcg
     1381 cgtgaacgcg gagacaacgt ctttcaggac cggggcttcg gcaatgcgtg tagccctcat
     1441 ggcgattgga ccgccaacat ccaacctttc gataacgcct ggactggctg agttggcggc
     1501 ggtagtgcgg aagcctcccc ggtccacaaa gacatatccg gaggataatc ccgtggcgga
     1561 actgaatttc tgcgtgggat aaagggacgc attcagcatc tggtggccga cggcgaacac
     1621 gtcgtagctg gaagccgcat agcctccgga caatgaggtt tcatcctttc gggaagcgat
     1681 gaagctatcc acgttcgcgt tgtagagcat gatggttccg ttggcttggg caaccaggat
     1741 cgcagagccg ttaggcgaag cggtcaggac cgtgtcggcg tcgacatcgt tcttatagat
     1801 gccgaggctg ggcagttcga cagccctgcg agtgcccaaa tccacccggt cgatcatatg
     1861 tatcgggccg gcaactcgcg atgcggcgag aatagcattc cccgaggcgg ctatggaacg
     1921 cggatagtgc ccggcaggga atacgatggg ctgctgcggc tccaatgtct caaggtcgaa
     1981 gacattcgcg atctgcgagt tgtcgttgcc aacaatcaaa tagtgccggt cgaagctgat
     2041 ggtcatctgc gttggcgtat tgccggttct cagcgtatgc agcaggctgt agttggtgcc
     2101 gtcatataca agaacctggt tcttatcctg gcgcagcacg aagaacctgt cccggtccgg
     2161 gtctgtcgca acgtctacca gcttgcccgg gacgttgatc accgtgccgc gctgatcggg
     2221 atcgcgcagg ttgacgagaa cacgaaccgt ctccggaatg ttcaccgcac cgggggacga
     2281 gatggtcagg gtggcggtga tcgtgcctcg ctggccttgg aatgagcccg cgtccacgcg
     2341 tacgcggacc gtcaggggag tcactccgga tgacggtgaa accgataccc ccgggcttga
     2401 agacgtaatc gcaaagggtg ttgctccgcc gccagggtcg gagataacca gttcctgcgt
     2461 ctggctgcgg cgatcgcagg cgttgctttg aaacaggaga tcctcgtgtt gcgcgcgaac
     2521 tcgtcggtat tggtcgagcg agccgacagg cagcaccaga actccactat cggatgccgc
     2581 atacatcgtt gagccgtctg cggacaacac actcttgccg gcgaggtttt ccggcaaacg
     2641 caacctctcc cgcaccgtca ggttatcgag gtcggcgacg agcaattccg gtggatccgg
     2701 tgttgtggag acaaccggcg tgccggagga cgacgagtcc gagggcacaa ccggcgccgc
     2761 gcgcgggacc tggccataga agatcgaacg ctcggaatcg atcgcgtgac ctccggtgtt
     2821 cagcgagccg gatgcatccc gaaattgggc catcaggaca tcggaagcgc ctaccggccg
     2881 gaatacggcc catcccgcgg cgtagatttg gccatcgcgc gatacactta ccacgcgcgg
     2941 ccccagcggc ggcgtggccg tatatcccag tgaaatgacc tgtttcatgc ggacgtcgta
     3001 gcggaagcgg atcgtatcgg tcaatccgaa gaccatcgtg tggtccgccg aagcggcaac
     3061 cgaagcggca acgatctggg gaggaaactt cggaggcggc accggcaagg tattcgccgt
     3121 cacgccctct accgtggcca gcacctgcgt agtgccaagc agcggatcga agagcacgaa
     3181 ttcattcgtg gtaacgatca atgcgagacc atcgattcca aacgcgacgc ccagcggagg
     3241 gttgcccatg gcgaaggtct tccggtcatt cgtttccagg tcgatgacgg tcagcgcatt
     3301 cgaggaactg cccggtgcct ggaagtttcc gtaatgcgtg accacgagat acctaccgtc
     3361 gggcgacagc gccatcgatg ccggttgtgg agccacattc atcgaagccg gctcgagcga
     3421 gcggctggcc agagacatga tttcaatccg gttcgctccg aaattggcaa tgtacaaacg
     3481 tcctcgcgcc tcatcgagcg caaggtctgc cgcgtgccct ccgatcacaa caacttttcc
     3541 gaaggttccg gcggatgccg ctgctaccaa aagcaaaccg agcatcaaga gcttccaaca
     3601 cccattcgtt ttcatacaag atccctgtta ccagccggca caaagttcca tttgtggggg
     3661 aacaggcggc gagtctccaa tgctcccccg aaagatagta tttattcggc gcgccttctg
     3721 cgacgcagaa taatgagtcc catcaaaacg gccgccagca cagcgcacac aattcgaatt
     3781 gtcgtcggat ccatacgtca tttctccttt ttaatcaaat gctcagatgt tattcatcat
     3841 cgggatcagc tcccggacga ttttgactgc catcggacca accgtcacca cgaacaggga
     3901 cggcaatacg cagaaaaaga tggggaagag cagcttgacg cccagcttgg ccgccttctc
     3961 ctcggcaact tgtctggcct gtattcgcat gtagtcggca tgcccacgga gactttgtga
     4021 aacgccggtt ccgaaccggt ccgcctggat gagcaccgct acgagtttt
//
LOCUS       DASTMK010000563        10954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_317409, whole genome shotgun sequence.
ACCESSION   DASTMK010000563 DASTMK010000000
VERSION     DASTMK010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317409"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFQ79_23150"
     CDS             complement(<1..937)
                     /locus_tag="VFQ79_23150"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0142639.1"
                     /translation="MNDAPLLLKETRRHFFERCGTGVGKLALASLLGRHHPAFAEMLR
                     HGGAPHHEARAKSVIFLFMAGGPSQLDLFDYKPLLQKHDASPAPDSLLAGKRFAFMDT
                     FSKERPKLLGSRRKFQQHGESGAWVSDCFPHLAGVVDSLTFLRTVATDNFNHAPAKIF
                     VNTGSTRFGRPSMGSWVTYGLGSECEDLPGFVVLQSGPRGPRGGALNWGSGFLPTRYQ
                     GVPFRSAGDPIVDLKNPEGVTRRSQRASIDLIRDLNRMRLDATGDDEISTRIASFEMA
                     YRMQTSAPELIDLSGETSETLALYGIERGAPSFAAN"
     gene            complement(942..3446)
                     /locus_tag="VFQ79_23155"
     CDS             complement(942..3446)
                     /locus_tag="VFQ79_23155"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEU0142640.1"
                     /translation="MRQAAIAVLAFAYGAFAQPPDAAKSSEWFESTVKPVLRANCWAC
                     HSARNRTSGLALDSRDAILAGGNRGPAVRPGQSAASLLMAAVRHEGAVKMPPTTRLKE
                     EQVEVLRAWIDAGVPWSGTSAKIPGADHWAFQPIRRPAIPEVRNSAWTRNAIDRFIAA
                     RLEKENLQPSPEADKYTLLRRVSLDLTGLPPSPAGMDAFLADSSPDAYERVVDRLLAS
                     PHYGERWGRHWLDLARYADSDGYNIDSPRPMWKYRDWVINALNGDMPFDQFVIEQIAG
                     DLLPHPTADQLIATGFHRNTLLNLEGGIDFEQYRVEAVADRVDTTGVVFLGLTVGCAR
                     CHDHKYDPVSQRDFYRLYAFFNNIDEMTNGRDRGRAHEPVLEFPTRDQSIQRETIRKQ
                     IALLEEELKLYQESLDSKFPVWEKEVRAHPPEKMTPELLAAVNRDVAGRTEIERDELM
                     RFFHEQDLGWKERRASIAALRRREPKVDAALILRELPSPRESYIHLGGDFLRKGEPVE
                     PGTPQVLPQLPGIGESTRLDLARWLVSKQNPLTSRVMVNRVWQRYFGRGLVATENDFG
                     TQGAEPVHPDLLDWLASEFMASGWKLKVLHRTIVTSAAYRQASANRSDLAQRDPGNQL
                     VARQHRLRLEAEIIRDAALAASGLLSPKIGGPSVYPPLPEGAMGVTQVNREWPVSKGE
                     DRYRRGMYTFFWRSAPHPALMAFDAPDATAACTRRNRSNTPLQSLTTLNNEAFFEAAR
                     HLARRIVKESAAEDTARVEHAFRLVLARPPSPGERDRLITFVQTEQRYFEANEPEVAA
                     LAADPGMRRSRDLAFIAAWISASRALLNLDEFLTRE"
     gene            3538..5028
                     /locus_tag="VFQ79_23160"
     CDS             3538..5028
                     /locus_tag="VFQ79_23160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU0142641.1"
                     /translation="MDERSLVFETVKQIDRRKFLLGAGSGLLAASSALSCRRESAKGT
                     GRPNVVFILADDLGYRDVGCYGQDKIRTPNIDRLAAEGLRFTDAYAGCTVCAPSRSVL
                     MTGLHTGHTPIRSNGMGSLLAEEKTVTEIFRAAGYATGGFGKWGLGEEPGAPGVPSRK
                     GFDQFFGYLNQAHAHFYYPEFLYDNEWEYPLKGNEGGKRTTYSHDVIAQKALDFVRQN
                     HKTPFFCYVPFTLPHWELLVPEDSLAEYRGKFEERAFIDPRDHYARQDESRAAYAAMI
                     TRMDRDIGRLMSLLKELDIDENTIVFFSSDNGPAMPIRGDDYFRSKGEFRGHKGNFYE
                     GGIRVPLIARWPGRVPADTVTDLACGFQDFLPTAAELIGAEAPPDIDGVSLVPTLLGS
                     ETTGRRQQAHEYLYWELPRRDTKDRWNYRKETPPQALRAGEWKAVRPQPDGPVEVYNL
                     KDDPGEQKDLAAANPEVKARLEGLLKTARTEPRPAVPFKDRWRNQS"
     gene            5047..6228
                     /locus_tag="VFQ79_23165"
     CDS             5047..6228
                     /locus_tag="VFQ79_23165"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142642.1"
                     /translation="MNRRTFLGAGTALSYNAILGANDRVRAGIIGAGGRGRYLTGEFK
                     EIGAEMTAVCDVYEPNLQAGLKVANTGAKSYQDYRKLLEDKSIDAVVVATPDHWHARM
                     VIDAVNAGKDVYVEKPMAHQIPEGFDVIDAVRRTKRIVQVGTQRRSFDLFQDAKRIMD
                     SGQLGNVHLVTSYWMNYTDRLSDRKLEGDLDWQKWLGTAPKRELDPLRFFNWYYFFDY
                     SGGLLVGQAAHVVDAIQWFMNSNAPLAVTCTGGQGNLPGAEVPETATIAIEYPENYLA
                     TFTLGYKAMRYNAFNDQLKQFHGTKARFDVGREWYALYPQSNAVEMKPSVEEKRPGSF
                     GPASRAHIRNFLECIGSRKEPNAPVEAGQATNIVLCLAMDSLRAGKRLKWNAKSRKVE
                     S"
     gene            6348..8282
                     /gene="dnaK"
                     /locus_tag="VFQ79_23170"
     CDS             6348..8282
                     /gene="dnaK"
                     /locus_tag="VFQ79_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU0142643.1"
                     /translation="MSKIIGIDLGTTNSVVSVMEGGQPVVVPNQEGGRTTPSVVGFTK
                     SGERLVGQVAKRQAVTNPENTVYSIKRFMGRRIDEVNEEMKMVPYKVTAGDNRDVRVE
                     AAGKRYSPPEISAMILGKLKEAAEAYLGEKVTKAVITVPAYFNDSQRQATKDAGRIAG
                     LEVMRIINEPTAAALAYGLDKKKDETIAVYDFGGGTFDVSILEVGDGVVEVKSTHGDT
                     HLGGDNIDQRIIDWIVSEFKKENGIDLSKDQMALQRLKEAAEKAKIELSTLLESEINL
                     PFITADATGPKHLVMKLTRARFEQMADDLLQRTIGPCKQALSDAGLTPDKIDEVVLVG
                     GSTRIPKVQQIVRDLFGREPHKGVNPDEVVAIGAAVQGGVLGGEVKDVLLLDVTPLSL
                     GIETLGGVFTKLIERNTTIPTRKSETFSTAADSQTSVEIKVYQGERAMASHNRLLGVF
                     QLANIPPAPRGIPQIEVTFDIDANGILNVTAKDKATNNEQKITITSSSGLSKDEVEKM
                     AKDAEAHASDDRKQRDTIEARNRADAMVYNVEKMLKEHRDKIAESDAKDVEAALEETK
                     QAIGQGDLAQINAAADKLTQASHKLAEAMYKSSAGQPGGPGPGAGGPQTDGGPSAQGP
                     SEGKDDVVDAEFVDVDDKNK"
     gene            8297..9529
                     /gene="dnaJ"
                     /locus_tag="VFQ79_23175"
     CDS             8297..9529
                     /gene="dnaJ"
                     /locus_tag="VFQ79_23175"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU0142644.1"
                     /translation="MAGLISRPAGFIDGSSVMAQQDYYKVLGVGRSASEEEIRKAYRR
                     LARKHHPDLNPGDKAAEDRFKKVQEAYDILSDPKKKQMYDQYGFYSDAGFAGAGGGTP
                     NAGQGGFGFGGFDFSDFAGAGGGSTGGGTGRGEGGFGNFRDLFGQFFRGGAAAGSERS
                     STPERGADLEYALSIGFWESVRGTQVKLSITRQDVCATCNGSGSAGGRTMVCPQCNGT
                     GNVTQMAGSMRFNLTCPRCGGSGKLRDACPTCHGDGRVAQTETVDVRIPPGAQTGSRL
                     RVAGKGNAGTLGAASGDLYITIRVEPHPFFEREGDDIQIRVPVSISEAGLGAKIEVPT
                     IDGRALLKIPQGTQNGQKFRLREKGVLNARKNKRGDQIVEVVLKAPEVRDERTREILR
                     ELAEIHPEDPREEIWAKV"
     gene            9531..9935
                     /locus_tag="VFQ79_23180"
     CDS             9531..9935
                     /locus_tag="VFQ79_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0142645.1"
                     /translation="MAKGKSRAAYMISAVADQYGIHPQTLRLYEREGLLRPSRSEGNT
                     RLYTKEDLERLEVILQLTRELGVNLAGVEIILNMREKMETMQRQMEEFIRMLNSELAS
                     RRTAPGDAPRHSLVRVIQVPSRKMRGNGPEAG"
     gene            10018..>10954
                     /locus_tag="VFQ79_23185"
     CDS             10018..>10954
                     /locus_tag="VFQ79_23185"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0142646.1"
                     /translation="MAMGTRKKRERQEGLWIASNAVVEPPGNVFYDRLNRILDEHKFD
                     QKVEALCRKFYKKSPYGRPSIAPGVYFRSLLVGYFEGLDSERGIAWRAADSLSLRKFL
                     GYALDEETPDHSTISRTRRLYWLETHKAVFRWVIRILTGEGLISGQTISIDATTLEAN
                     AAMKSIIRRDNGQSYNDYLKGLATAAGIENPTREQLARLDRKRKKKGSNEEWQSPADP
                     DARITKMKDGRTHLAHKAEHAVDLSSGALLAVTLQPACEGDTSTIHKTLAETQSAARE
                     IDENGVEEVVADKGYHSGEVLKDLHEQGVRTYIPEP"
BASE COUNT         2357 a         3074 c         3572 g         1951 t
ORIGIN      
        1 agttggccgc gaacgaaggc gcaccgcgct cgatgccgta cagggcaagt gtttcggaag
       61 tctcgcctga aagatcgatc aactccggag cgctggtttg catccggtag gccatctcaa
      121 acgaggcgat gcgcgtcgag atctcatcgt cgccggtggc atccaagcgc atacggttca
      181 gatcgcggat caggtcgatg gaggcccgct ggcttcgccg tgtaacccct tccggatttt
      241 tcagatcgac gatcgggtct ccggcagagc ggaatggcac accctgatag cgcgtcggca
      301 gaaacccgct tccccaattc aacgccccac cgcgcggccc gcgaggcccg gattgcagaa
      361 cgacgaaacc gggcagatcc tcgcactcgc ttcccaatcc ataagtgacc caggagccca
      421 tgctcggacg gccaaagcgt gtagagcccg tgttgacgaa aattttcgca ggcgcgtgat
      481 tgaagttgtc cgtcgcaacc gtccggagga acgtgaggct gtccactact ccggcgagat
      541 gcgggaagca gtccgaaacc catgctccgc tctcaccgtg ctgttgaaac ttcctgcggc
      601 tgcccagcag cttgggacgc tccttcgaaa acgtatccat aaacgcgaag cgcttgccgg
      661 cgagaagaga atccggagcg ggcgaagcat catgtttttg cagcagcggc ttgtaatcga
      721 aaaggtccaa ctggctgggg ccacccgcca tgaacaggaa gattacactc tttgcgcgag
      781 cttcatgatg cggagcaccg ccgtgccgca gcatctccgc gaaggccgga tgatggcggc
      841 ccaacaggct ggcgagcgcg agtttgccga cgccggtacc acagcgttcg aagaaatgtc
      901 ttcgggtttc cttcagcagc agcggcgcgt cattcatggc gttattctct tgtcagaaat
      961 tcatcgagat tgagtagcgc cctggatgcc gatatccacg cagcgataaa agcgagatcc
     1021 cgggagcggc gcattccagg atcggctgcg agggccgcca cttccggttc gttcgcctcg
     1081 aagtaccgtt gctccgtctg gacaaaggtg atcaggcggt cgcgttcacc gggtgatggc
     1141 ggccgcgcca ataccagccg gaatgcgtgt tccacgcgag cggtgtcctc cgcggcgctc
     1201 tccttcacga tacggcgcgc aagatgccgg gccgcttcaa aaaacgcttc gttgttcaac
     1261 gtggtgagcg attgcagggg agtgttcgag cggttccgcc gggtacaggc cgccgtggca
     1321 tccggagcgt cgaaggccat cagcgccggg tgcggtgcgg accgccagaa gaaagtgtac
     1381 atgccgcggc gatagcggtc ctctcctttg ctcaccggcc attcgcgatt tacctgcgtt
     1441 acgcccatcg cgccttcggg caggggcgga taaacgctcg ggcctccgat tttcggcgac
     1501 agcaagccgc tggccgccag agccgcatcg cggatgattt cggcttccag gcgaaggcgg
     1561 tgctggcggg cgacgagctg attcccgggg tcgcgctgtg caagatcgga gcggttggcg
     1621 gacgcctggc gatatgcggc cgaagttacg atcgtgcggt gcagcacctt cagcttccac
     1681 cctgaagcca tgaattcgga cgcaagccaa tcgagcagat cgggatgcac aggctcggct
     1741 ccctgcgtac caaagtcgtt ttcagtcgcc accagccccc ggccgaaata gcgctgccag
     1801 acacgattga ccatcacgcg ggaggtgagc gggttctgct ttgatacgag ccagcgcgca
     1861 aggtcgagcc gggttgactc gccgatgccg ggaagttgag gcagaacctg cggtgtccca
     1921 ggctcgacag gctcgccttt gcggaggaag tcgccgccaa ggtggatgta gctctctctg
     1981 ggcgatggca attcgcgcag gatgagagcg gcgtccacct tcggctctcg tcttcgcagc
     2041 gccgcaatgg aagcgcggcg ctccttccac ccaaggtcct gctcatggaa gaagcgcatc
     2101 agctcatcgc gttcgatttc ggtgcgcccg gccacatcgc ggtttaccgc ggccagcagt
     2161 tccggcgtca ttttttcggg cggatgggcg cgcacctctt tttcccatac cgggaatttc
     2221 gaatcgagag actcctgata gagtttgagc tcttcttcga gcagcgcaat ctgcttgcgg
     2281 atcgtctcac gctgaatgga ttgatcgcgc gtcggaaact cgagcacggg ttcatgcgcc
     2341 cggccgcggt ccctgccgtt cgtcatctcg tcgatgttgt tgaagaacgc gtacaagcga
     2401 tagaaatccc gctgggagac cgggtcgtat ttatgatcgt ggcagcgggc gcatccaacc
     2461 gtgaggccga ggaacacaac gccggtggtg tcgacacggt ccgcaaccgc ttccacccga
     2521 tactgctcga aatcgatgcc gccctccagg ttcagcagcg tgttgcgatg aaagccggtc
     2581 gcaatcaatt gatcggcagt cgggtggggg agcaggtcgc cagcgatctg ttcgattacg
     2641 aactgatcga acggcatgtc gccattgagc gcgttgatga cccagtcgcg atacttccac
     2701 atggggcgcg gactgtcgat attgtatccg tcggaatcgg cgtagcgcgc gaggtcgagc
     2761 cagtggcggc cccatcgttc accgtaatgg ggtgaagcaa gcagccggtc cacgacacgt
     2821 tcgtacgcat ccggagacga gtcggccaga aacgcgtcca tcccggcggg tgaagggggc
     2881 aagccggtaa ggtcgaggct gacgcgccgc agaagcgtat atttgtccgc ttccggcgac
     2941 ggctgcaagt tttctttctc gagccgcgcg gcgatgaagc ggtcgatggc attgcgcgtc
     3001 cacgcggagt ttcgcacctc gggaatggcc ggccgccgga taggttggaa cgcccaatga
     3061 tccgccccgg gaatcttcgc ggatgtgccg gaccagggca cgccggcatc gatccaggcg
     3121 cgcaggacct cgacctgctc ctccttaagc ctggtagtgg gcggcatttt caccgcgccc
     3181 tcgtgccgca cggcagccat caacagactt gcggcgctct gtccggggcg caccgccgga
     3241 ccccggttgc cgccggcgag aatggcgtct ctggaatcaa gggcaagtcc gctggtccgg
     3301 tttctcgcgc tgtggcacgc ccaacagttc gcgcgaagca ctggtttcac ggttgattca
     3361 aaccactcgg aggatttggc ggcgtccgga ggctgcgcga acgcaccgta agcgaatgcg
     3421 agtaccgcga tagcagcctg cctcatggtt tcagcccagc acgaatatgg attaatctta
     3481 cagaattcgg gtaggaagcg cattgggagt ttgctttgcg tccgccgaag gatatagatg
     3541 gatgaaagga gcctggtgtt tgaaaccgtg aaacagatcg atcgccgcaa attcctgttg
     3601 ggagcaggca gcggattgct tgccgcctca tcggcactgt cctgccgcag agagagcgcg
     3661 aagggcacgg gacgcccgaa cgttgttttc attctcgccg acgatctcgg atacagagac
     3721 gtggggtgtt acggacagga caaaatccgg acgcccaaca ttgatcgctt ggccgccgag
     3781 ggcctgcggt tcaccgatgc ctacgcggga tgcacggtct gcgctccgtc gcgaagcgta
     3841 ttgatgacgg gccttcacac cggccatacg ccgatacgca gcaatgggat gggttccctg
     3901 ctggcggaag agaagacggt tacggagatc tttcgcgcgg ccggttacgc gacgggcggc
     3961 ttcgggaaat ggggacttgg cgaagagccc ggcgctccgg gagtcccttc ccgcaaaggc
     4021 ttcgatcagt tcttcggcta tttgaaccag gcccacgcgc atttctacta tcccgaattt
     4081 ctttacgaca acgagtggga atatccgctg aagggcaacg aagggggaaa gcgcaccacc
     4141 tattcgcacg acgtcatcgc gcagaaggca ctcgattttg tccggcagaa ccataagacg
     4201 ccgtttttct gctatgtccc gttcacactt ccgcattggg agttgcttgt gcccgaggat
     4261 tcgctggcgg agtatcgcgg caagttcgag gaaagagcgt tcatcgaccc gcgggatcac
     4321 tatgcccggc aggacgaatc ccgtgccgcc tatgcggcga tgatcacgcg catggatcgc
     4381 gacatcggcc gcctgatgag ccttttaaag gaattggaca tcgacgaaaa caccatcgtg
     4441 ttcttcagca gcgacaatgg gcccgctatg ccgatccggg gcgacgacta cttccgcagc
     4501 aagggcgagt tccggggcca caagggcaac ttctatgaag gcggcatccg ggtccctttg
     4561 attgcgcgtt ggccggggcg cgtgccggca gacactgtca ccgatctggc ctgcggattt
     4621 caggacttcc tgccgaccgc ggccgagttg atcggcgccg aagcaccacc ggacattgat
     4681 ggtgtctcgc tcgttccgac tctgctcggg agcgagacca ccggccggcg gcagcaggcg
     4741 catgaatatc tttattggga actgccgcgg cgggatacga aagaccggtg gaactaccgg
     4801 aaggaaaccc ctccgcaggc gctccgggcg ggtgaatgga aagctgtgag gccgcagcca
     4861 gatggaccgg tcgaggtcta caatctgaaa gacgacccgg gagagcagaa ggatcttgcg
     4921 gcagcaaacc cggaggtgaa ggcgcggctc gagggccttc tgaagacggc cagaacggag
     4981 ccccgtccag cggttccgtt caaagatcgc tggcgcaacc aatcgtaaga agagtgagga
     5041 gatactatga accgtagaac gtttttgggc gccggcacgg cgctttccta caacgcgatc
     5101 ctgggagcca atgaccgcgt ccgggcaggc attatcgggg cgggcggccg gggccgctat
     5161 ctgactggtg aattcaaaga aatcggcgcg gaaatgacgg cggtctgcga cgtttacgag
     5221 ccgaatctac aggccgggct gaaggtagcc aacaccggtg cgaaatcgta tcaggattac
     5281 agaaaactgc tggaagacaa gtccatcgac gcggtcgtcg tcgccacgcc ggaccactgg
     5341 catgcacgga tggtgatcga tgcggtcaac gcgggcaaag acgtatacgt ggaaaagccg
     5401 atggcgcacc agatccccga ggggttcgac gtgatcgacg ccgttcgccg caccaaacgc
     5461 atcgtgcagg tcggcaccca gcgaagaagc ttcgacctgt tccaggatgc caagcggatc
     5521 atggactcgg gccaactggg caacgtccat cttgtgactt cttattggat gaactacacg
     5581 gacaggctgt cggaccgcaa gttggaaggg gacctggact ggcagaagtg gctgggcacg
     5641 gcgcccaagc gcgaactgga cccgctacgg ttcttcaact ggtattactt cttcgattac
     5701 tcgggcggct tgctggtagg acaggcggcg catgtggtgg atgcgattca gtggttcatg
     5761 aattcaaacg cgccgctggc ggtgacgtgt accggcggac aagggaacct gccgggtgcg
     5821 gaggtcccgg agacggctac cattgccatc gagtatccgg agaactacct ggcgacgttc
     5881 actctggggt ataaggcaat gcgctacaac gcctttaacg accaactgaa gcagtttcac
     5941 ggcacaaagg cgagatttga cgtggggcgg gaatggtacg cgctgtatcc ccagagtaat
     6001 gcggtcgaga tgaagccgtc ggtggaagag aagcgtccgg gaagtttcgg gcctgccagc
     6061 cgcgcgcaca tccgcaactt cctggaatgc atcgggagcc ggaaagagcc gaatgccccg
     6121 gtcgaagccg ggcaggcgac gaatatcgta ctgtgcttgg ccatggactc acttcgtgcc
     6181 gggaagcgtc tgaagtggaa tgcgaagagc cgcaaggtag agagctaaac ttgacaataa
     6241 tagactaagc tttcataata gaaatgtgct aactgttggg tggcctgtag cgccctccac
     6301 cggcgcaatc ttatttttac ggtcttattt tctggaggac gaacggaatg agcaagatta
     6361 tcgggatcga tctgggtacc acaaattcgg tggtttccgt catggaaggc ggacaaccgg
     6421 tggtggttcc caatcaggag gggggccgga cgacgccctc cgtggttggt tttacaaaaa
     6481 gcggtgagcg cctggtgggg caggtcgcca aacgccaggc ggtgacgaac ccggaaaaca
     6541 cggtttactc gatcaagcgg ttcatgggac gccgcatcga cgaagtgaac gaagaaatga
     6601 agatggtgcc gtacaaggtg acggccggag acaaccggga cgtacgagtg gaggctgccg
     6661 gaaagcgcta ctccccaccg gagatttcgg ccatgatcct gggtaagtta aaggaagcgg
     6721 ccgaagccta cctgggcgag aaggtcacca aggccgtcat caccgttcca gcttatttca
     6781 acgactcgca gcggcaggcc acaaaagacg cgggccggat tgccgggttg gaggtgatgc
     6841 gtatcatcaa cgagcccacg gcggcagcgc tggcttacgg tctcgacaag aagaaagacg
     6901 agaccatcgc cgtctacgac tttggcggtg gtacttttga cgtgtcgatt ctcgaagtgg
     6961 gcgacggagt ggtggaggtc aagtccaccc acggagatac acacctcggc ggcgacaaca
     7021 tcgaccagag aatcatcgac tggatcgttt cggagttcaa gaaagagaac ggcatcgatc
     7081 tttcaaaaga ccagatggcg cttcagcggc tgaaggaagc ggccgagaag gcgaagatcg
     7141 aattgtcgac gctgctcgaa agcgagatca acctgccgtt catcaccgcg gatgccaccg
     7201 ggccgaaaca cctggtgatg aaactgaccc gcgcgcggtt cgagcagatg gcggacgacc
     7261 tgttgcagcg caccatcggg ccgtgtaaac aggcgctgtc ggacgcgggg ctcacgccgg
     7321 acaagatcga tgaagtagtg ctggtgggtg gttccacgcg aattccgaaa gtacagcaga
     7381 tcgtgcgcga ccttttcggc agagagccgc acaagggcgt gaacccggac gaggtggtcg
     7441 cgatcggcgc ggccgtgcag ggcggcgtgc tgggcggcga ggtaaaggac gttctgctgc
     7501 tcgacgtgac accgctttcg ctcgggatcg aaacgctcgg cggggtgttt accaagctga
     7561 tcgagcgcaa cacgacgatt cccacgcgta agagcgaaac gttttcgacg gcggccgaca
     7621 gccagacctc ggtagagatc aaggtttacc agggcgagag ggcgatggcg tcgcacaacc
     7681 ggctgctcgg tgtgtttcag ttggcgaaca ttccaccggc gccgcgtggc attccgcaga
     7741 ttgaagtgac tttcgacatc gatgccaacg gcattctgaa cgtaacggca aaggacaaag
     7801 cgaccaacaa cgagcagaag atcacgatca cgtcttcgtc tggcctcagc aaagacgagg
     7861 tggaaaagat ggcgaaggat gccgaagcgc acgcctccga cgaccgcaag cagcgtgaca
     7921 cgatcgaggc gcgaaaccgg gccgacgcga tggtctacaa cgtcgaaaag atgttgaaag
     7981 agcatcggga caagatcgcg gagtccgacg ccaaggacgt cgaagcggcc ctcgaggaga
     8041 cgaagcaggc gatcgggcag ggcgacctgg cgcagatcaa cgcggcggcc gacaagctga
     8101 cacaggccag ccacaaactg gccgaggcca tgtacaaatc ctcggccggg cagcccggcg
     8161 gacctggacc cggcgcgggc ggtccacaga cggacggcgg accgtcggcg cagggtccgt
     8221 ctgaaggcaa ggacgacgtc gtggacgcgg agtttgtaga cgtagacgat aagaacaagt
     8281 aagcgttgga tccggtttgg cgggcctcat atcaaggccc gccggcttta tcgatggttc
     8341 gagtgttatg gcccagcagg attattacaa ggtgctaggg gttggtcgca gtgcgtccga
     8401 ggaggagatc cggaaggcgt accgccggct ggcccgcaaa caccaccccg acttgaatcc
     8461 cggcgacaag gccgccgaag accggttcaa gaaagtacag gaagcgtacg acattctgag
     8521 cgatccgaag aagaagcaga tgtacgatca gtacggcttc tactcggacg cgggattcgc
     8581 cggggcgggc ggcggcactc cgaatgccgg gcagggcgga ttcggattcg gcggcttcga
     8641 tttttcggac tttgccggcg cgggcggcgg tagtacgggc ggcggcacgg gacgcggaga
     8701 aggtggattc ggcaacttcc gcgatctgtt cggccagttc tttcgaggag gcgccgccgc
     8761 cggctccgag cgttcttcca cgccggaaag aggcgccgat ctcgagtatg ctctcagcat
     8821 cggattctgg gaatccgttc gcggcacgca agtgaagttg agcatcacca ggcaggatgt
     8881 ctgcgcaacg tgcaatggat cgggttcggc gggcggacgg acgatggtat gcccccagtg
     8941 taacggtacc gggaacgtta cgcagatggc gggctccatg cggttcaacc tgacgtgtcc
     9001 ccgatgcggc gggtccggca agctccgcga tgcatgcccc acgtgtcatg gagacgggcg
     9061 tgtggcacag acagagaccg tagacgtacg cattccaccg ggggcgcaga ccggctcgcg
     9121 gctgcgcgtg gcgggaaagg gaaatgccgg gacgctcggc gctgcgagcg gcgacctgta
     9181 tattaccatc cgggtggagc cgcatccttt cttcgaacgc gagggagacg acatccagat
     9241 acgggtgccg gtctcgattt cggaagccgg tctcggcgcc aagattgaag taccgaccat
     9301 agatggccgc gcactgctga aaattccaca gggcacccag aacggacaga agttccggtt
     9361 acgggagaag ggcgtgctga atgcgcggaa gaacaagcgg ggcgatcaga ttgtggaggt
     9421 cgtgctgaaa gcgccggaag tgcgggacga gcggactcgt gagattctcc gcgaactcgc
     9481 cgaaatccat ccggaggacc ccagagagga gatctgggcg aaggtgtgat atggcgaagg
     9541 gcaaatcaag agcggcttac atgatttccg cggtggcgga tcagtatgga attcatccgc
     9601 agactctccg tctttacgag cgcgaagggc ttctacgtcc ttcccgcagc gaagggaata
     9661 ctcgcctcta caccaaggaa gacctggaga ggctggaggt gattcttcag ttgacgcggg
     9721 agctcggagt caacctggcg ggcgttgaaa tcatcctgaa tatgcgggaa aagatggaaa
     9781 cgatgcagcg gcagatggag gaattcatac gcatgttgaa ctccgagctt gcttcgcggc
     9841 gcactgcgcc cggcgacgcc ccgcgccatt cgctggtccg cgtcattcag gtccccagcc
     9901 ggaaaatgcg cggaaacggc ccggaggccg gctagaagcc cgtggtttta gtcaggcgaa
     9961 tgcctaaaaa cgtctaagac atgtgacctc ggaaatgttt ccatcgtctt agctgacatg
    10021 gcgatgggaa cgcgaaagaa gcgggagcgg caggagggac tgtggattgc cagcaacgcc
    10081 gtggtcgagc ctccgggcaa cgttttttat gaccggctga atcggattct ggacgagcac
    10141 aagtttgacc agaaggtgga ggcgctctgc cggaagttct acaagaagag cccttacggg
    10201 cggccgagta tcgcgccggg cgtgtacttc cggtctctgc tggttggata tttcgagggc
    10261 ctcgattcgg aacgaggcat cgcctggcgc gcggccgatt cgctgtcgtt acgcaagttt
    10321 ctgggttatg cgctggatga ggagacaccg gatcactcga ccatctcgcg cacgcgccgc
    10381 ctgtactggc tggagacgca taaggcggta ttccgctggg tcatccgaat tctgaccggg
    10441 gaaggactga tcagcggaca aacgatctcg attgacgcca cgacactgga agccaacgcc
    10501 gcgatgaaga gcatcatccg gcgggacaac gggcaaagct acaacgatta tctgaaagga
    10561 ctggcaacgg cggcaggcat cgagaacccc acgcgcgagc agttggcgcg gctggaccgg
    10621 aagcgcaaga aaaagggctc caacgaggag tggcaaagcc cggccgatcc ggatgcacgc
    10681 attacgaaga tgaaggatgg ccggacgcat ctggcccata aggccgaaca cgcggtcgat
    10741 ctgtcgagtg gcgcgctgct ggcggtcacg ctgcagccgg cctgcgaggg agacacgagc
    10801 accattcaca agacactggc cgaaacacag tccgccgccc gcgagatcga cgaaaatggg
    10861 gtggaagagg tggtggccga caaaggctat cacagcggcg aagtgctgaa ggatcttcac
    10921 gagcaagggg tacggaccta tatccctgag ccag
//
LOCUS       DASTMK010000564        10002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_444867, whole genome shotgun sequence.
ACCESSION   DASTMK010000564 DASTMK010000000
VERSION     DASTMK010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444867"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1243)
                     /gene="queG"
                     /locus_tag="VFQ79_23190"
     CDS             complement(200..1243)
                     /gene="queG"
                     /locus_tag="VFQ79_23190"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU0142647.1"
                     /translation="MQSEEVKRLARGCGFQLAGVAPVEPVREAGFFLDWAARGLAGTM
                     SYLTDRRAEIRDSARSLLPSARSVICAGKLYNAPQPYSTRFNEPERAWISRYAWGNDY
                     HEVLRRGLTQLLESLKQSVAEPFEAKICVDTAPILERAHAHRAGLGWIGKNTCLINQQ
                     QGSWFFLGELLVSLDLEPDTTPPDRCGSCTRCIDACPTEALVPVIYPGGPRYELDSRR
                     CISYFTIEHRGGVPAEHRPGLGHHLFGCDICQDVCPWNRMAPETAEEWCQPREFAPPL
                     DQFAAITENEFQTMFRGSPLKRARYTGFLRNVAIAMGNSGHSRFRDPLMALAGFPDAV
                     VAETAEWALRQLR"
     gene            1461..3566
                     /locus_tag="VFQ79_23195"
     CDS             1461..3566
                     /locus_tag="VFQ79_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0142648.1"
                     /translation="MHKPIKYVEKGLSLAANAAWIAFDKLNQIKRNPSFTPKWSDKPL
                     LKSWEKSLPTLGWPRETDSLCPKCVPEIRQAVIDGKVPLETLIYEKPGEIKAQIIERD
                     GKIMMVKDCPVHGHFEDVMAIDTEFFAHLEKVFPGRDIRAHNDEKLHNHGSSTVTHGR
                     GSVLTIDLTNRCNMMCDPCFMDANQVGFVHELSWEDIKTLLDNAITIKPKRQMSVQFS
                     GGEPTLSPYFIDAVRYARKVGYTSVQAATNGIEFAKRPDFCHEAAEAGLRYVYLQFDG
                     IGDAANSHRHVGNLFEVKLRAIENLHSAGVDIVPVTTIINGINNEQVGRIIQFALDNP
                     KKIPFLSFQPVSFTGRDEAITDERRKAQRYTLSHMAHDVKRQTGLGEPKRDWYPISFI
                     STFSDWSDLVHGPAADWGQLSCGCHPNCGVGMAIMVDKETKEAVPVTQFLNADRLAED
                     VKRINDAARGKWLSVVGMALSVARNYDPFQSPTHFKLSDMMKKFDKTFGATGKSYGRV
                     DATRTREDIERRRSDRWNFLMIVGMWFQDLFNYDFRRTEQCIIPYATQQGEISFCAYN
                     TGMGWRNIVEKMHMTATLTKWYEEHGKHEIFAGGKSVALEENAHSLMLRDELVTKEEQ
                     RDLDLLGIAKTAREEKIRARDEKMREAQKNAEMAKLYRQHVLKEQPGPDLVQIGGIQP
                     APSNGALKEEREEVGSFGD"
     gene            complement(3603..6347)
                     /locus_tag="VFQ79_23200"
     CDS             complement(3603..6347)
                     /locus_tag="VFQ79_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142649.1"
                     /translation="MTSFFSKLKWLARRSSKEAELQEELQFHLEEEAGELEERGLASE
                     AARRAARRDLGNIALVQEDTRAAWTWIFWEQLVQDVRYALRTMTANKTFSALAILSLA
                     LGIGANTAIFSFLDSILLRSLPVSDPQSLVILSWHTPRDETRGTNRHHNSYADPNGGF
                     IGGFFSYPAFEFLKKNNTVFSSVFGYQGAGKLNLTFRGQAELVVTEYVSGDYFRGLGV
                     PPAAGRLIAPDDDRAGSAAVAVISFALSQRRFGGPENAPGQPVLINNLPFTVIGVTPP
                     EFFGADPDVAPGIYIPMHANLLLEAGDKRYPPTGIYANPSYDWVVPMARLRPGVTMVQ
                     AQATLATPFFEWGRAAKPNLKPEDVPTLVLREGAGGLDSLRRRYSKPLYILLTLVGLI
                     LAIACANIANLLLARAAARKREIAVRLSMGAGRFRVIRQLLTESILLAVAGGALGVGF
                     AVWGIRFLTLLLANGQEDFTLRAGLNWHVLGVVAGLSLLTGVLFGLAPALQATRVGLM
                     PALKQSRTGEVLAHGFRRLSLSRILMVSQIAISLFILVAAGLFARTLSNLTSIQLGFN
                     RENLLTFQLNARQAGHDDPGIVTFYNDLQRQFGAIPGVRAASLSNHMLIGTGWSATNV
                     GLPGGAIQNSRILLIGTGFFHTMQIPILLGREIEERDRQGSPMVAVVNEKFAKLSFGD
                     RNPLGQRLSLPRMCPKCDIEIAGVSGNSLYGNLKGDVPPIVYLPFAQGAWGPVREMHY
                     ALRTTGNPLGYTGAVRDIVRRADNRIPLSEVKTQSALIDRTINQEITFTRLCSAFALL
                     ALAIACVGLYGSMSYNVARRTGEIGIRMALGAQRGRVVWMVLREVCVVAAVGLAISLP
                     AALAASKVVESFLFGMKPNDPLALTASVVILISAAILAGYLPARNASRIDPATALRHE
                     "
     gene            complement(6344..6700)
                     /locus_tag="VFQ79_23205"
     CDS             complement(6344..6700)
                     /locus_tag="VFQ79_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0142650.1"
                     /translation="MKRKEPERVELLQGTLDLLILSTLMAGPNHGHAIAKHIQRTSED
                     LLQVETGSLYPALHRLEAKGWIAASWNLSDKGKRARYYRLTAAGRKQLANEQSKWEAL
                     ARAIGLIVKPVPQESQ"
     gene            6797..7363
                     /locus_tag="VFQ79_23210"
     CDS             6797..7363
                     /locus_tag="VFQ79_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142651.1"
                     /translation="MHPLLKSLTGGDRRSIGKSNHVASLVLEQPELIEVLVEGMESAD
                     PVLRTRSADAMEKVTAVRPELLVPYKKKLLQQFSRIEQQEVRWHVAPMLARLPLSEVE
                     ETAVLNILLSYTNDRSSIVKTMAMQALADIALRSLRFLPEVQRHIEELTATGTPAMKA
                     RGKKLLKQLAKPLRPAAGGESSHGSCRP"
     gene            complement(7545..8093)
                     /locus_tag="VFQ79_23215"
     CDS             complement(7545..8093)
                     /locus_tag="VFQ79_23215"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0142652.1"
                     /translation="MKRYVVTGAPGAGKTAIIRQLEIDGFSVVEEAATDVIALWQAKG
                     IAEPWARPEFINAVVSLQQTRERRAVCATDLVQFHDRSVVCTAALADYLGFSKPQNLL
                     QELRRVRTENVFQNRVLFLKNLGFVTPTEARRITYEETILFEQIHERTYRDLEFEIIP
                     IDSGSVSDRVKQIKRTLSLQPA"
     gene            8243..9307
                     /gene="rsgA"
                     /locus_tag="VFQ79_23220"
     CDS             8243..9307
                     /gene="rsgA"
                     /locus_tag="VFQ79_23220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU0142653.1"
                     /translation="MFLERLGADQTVYGAFSPYARQQMVLARVAVAQRDVYHLYTEDG
                     AAHAEPSGRLYYHTPGHSSFPVTGDWVAARIVGPEQALVEAVLPRRTCLSRRAAGRRE
                     EEQPLAANVDVVFLVCGLDGDFNLRRLERYLALVATSGASPVVVLNKADLCADPAGRI
                     ADTAAIARHVPVVAASTVDPHGLDALRRFLTPGGTVALLGSSGVGKSSIANRLLGEER
                     FRTGEVRMQDSRGRHITVRRELTPLPQTGALIDTPGMRELQLWTGPESIDAVFDDITA
                     IAQNCRFRDCTHSGEPGCAVAAALDRGQVDTDRWASFQKLRAEAGWHEAMSDPLTALE
                     RKRKWKIIHKQARAFNKSRR"
     gene            complement(9315..>10002)
                     /locus_tag="VFQ79_23225"
     CDS             complement(9315..>10002)
                     /locus_tag="VFQ79_23225"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU0142654.1"
                     /translation="DGKWLKAGPGTFVYGPREVPHGFKVIGHSPARMLVMCTPGGFEG
                     FVLEQTTPMTDPPSSPDMDKLMMLAAKYGIEIHGPLPPEPKSFEGEANPPADLKSLNH
                     RWIQAFNERDWRTERAVRGESFRAYLSGAAEPLDNDAWSRFMAAFTEGFPDSRIAIES
                     CIAEGDTVVTRWTLTGTHQEMFQGIPPTGRPVRFTGLEFNRVVGGRFVEHWSMFDNLA
                     LLRQIGAMAV"
BASE COUNT         2110 a         2985 c         2934 g         1973 t
ORIGIN      
        1 cattacgatt tcactacgct gagatgcctt gcggcccagt ccatcatgca agacatgcgg
       61 tgcatgatca gattattcca agatgcaatt gagtgtcaat tagaaaaaca ttagagcggc
      121 cttgcggcga tccgaaatgc aggaacggga cctcgaccgg gccgcacaac ccgcccaatg
      181 gcagttccgt gaaaaggctt taacgcaatt gcctgagggc ccattcggcc gtttcggcca
      241 cgacggcatc gggaaaacca gcgagcgcca tcaacggatc acggaatcgc gaatgcccgc
      301 tatttcccat cgcgatcgcg acgttgcgta aaaagccggt atatcgggca cgtttaagcg
      361 gcgagccgcg gaacatagtt tgaaactcat tttccgtaat cgccgcgaat tgatcgagcg
      421 gaggggcaaa ttcgcgcggc tggcaccatt cctcggccgt ctccggcgcc atgcggttcc
      481 acgggcatac atcctggcag atgtcgcaac cgaatagatg atgccccaat ccggggcggt
      541 gctccgccgg cactcctccc cggtgctcaa tcgtgaagta cgagatgcag cggcgcgagt
      601 ccagttcgta gcgcggcccg ccgggataaa tgaccggcac aagagcctcc gtcgggcagg
      661 cgtcgataca gcgcgtgcac gaaccgcacc ggtcgggcgg cgtcgtatcg ggttccaggt
      721 ccagggacac aaggagttcg cccagaaaaa accacgagcc ctgctgctgg ttaatcaggc
      781 aggtgttctt gccaatccaa ccgaggccgg cccggtgggc gtgcgcccgc tccaggatcg
      841 gggcggtatc gacgcagatt tttgcttcaa acggttctgc cacgctttgt ttcaggctct
      901 cgagtaattg ggtaagaccc ctgcggagaa cctcgtggta atcgttcccc caggcatagc
      961 gggagatcca ggcgcgctcc ggctcgttga accgtgttga ataaggttgc ggcgcgttgt
     1021 atagctttcc ggcgcaaatc acggaacgcg ccgagggaag cagcgaacgc gcgctatcgc
     1081 ggatttccgc tcgccggtcc gtgaggtagg acatggtgcc ggccagccct ctggccgccc
     1141 aatccagaaa aaatccggct tcgcggaccg gctccaccgg tgctactccg gccaactgaa
     1201 agccgcaacc ccgcgccagt ctctttactt cttcagactg catcgatttg attctgcgga
     1261 tgttatcaat gcccgcccgg ttccggagcc ggaccgccgt tcaacacgac atttcgccac
     1321 acaagcaaca aaatacaccc tttaactcaa ttctatagtc gcatcgcggt ggtggagcct
     1381 atactgcgtt tctggtgctg ggtttctggg ctgggaattc cggctggcgg ttaattttaa
     1441 atttcaggag taatcacgca atgcacaaac cgattaaata cgtggagaag gggttgtcgc
     1501 tcgccgccaa cgcggcgtgg atcgcgttcg acaagctgaa tcagattaag cgcaatcctt
     1561 ccttcacacc caaatggtcg gacaaaccgc tcctcaagtc ttgggagaag tccctgccga
     1621 ccctgggctg gccgagagaa acggactctc tctgcccgaa gtgcgttccc gagatccgcc
     1681 aggccgtgat cgacggcaaa gtgccgctcg agacgttgat ctacgagaag ccaggcgaga
     1741 tcaaggccca gatcatcgag cgcgacggca agatcatgat ggtgaaggat tgcccggttc
     1801 acggccactt tgaggacgtg atggcgattg acaccgaatt cttcgcgcac ctggagaagg
     1861 ttttccccgg acgcgatatc cgtgctcaca acgacgagaa gctgcataac cacggaagca
     1921 gcacggttac ccacggccgc ggctcggtgc tcaccatcga tctgacgaac cgctgcaaca
     1981 tgatgtgcga tccgtgtttt atggacgcca accaggtcgg gtttgtgcat gagctgtcct
     2041 gggaagacat caagacgttg ctcgacaacg ccatcaccat caaaccgaaa cggcagatgt
     2101 cggtgcagtt ttcaggcggc gagccgacgc tctcgcccta ctttatcgac gccgtccggt
     2161 atgcgcgcaa agtcggctac accagcgtac aggccgccac gaacggaatc gagttcgcga
     2221 agcggccgga tttctgccac gaagccgccg aggcgggtct ccgctatgta tacctccagt
     2281 tcgatggtat cggcgatgcc gccaactcgc accgccacgt gggaaacctg ttcgaagtaa
     2341 agcttcgggc gatcgaaaac ctgcacagtg caggagtcga tatcgttccg gtgacgacca
     2401 tcattaacgg aatcaataac gagcaggtgg gacgcatcat ccagttcgct ctcgataatc
     2461 ccaagaagat cccattcctg tccttccagc cggtttcgtt caccgggcgt gatgaggcta
     2521 tcaccgacga gcgccgcaaa gcacagcgat acacgctctc gcacatggct catgacgtga
     2581 aaaggcaaac cggtttgggt gagcctaagc gcgactggta tcccatttcc ttcatcagca
     2641 ccttctccga ctggagcgac ctggtgcacg gcccggcagc ggactggggc cagttgagtt
     2701 gcggatgcca tccgaactgc ggtgtcggaa tggccatcat ggtggataaa gagaccaagg
     2761 aagcggtgcc cgtcacgcaa ttcctgaacg ccgaccggct tgccgaggat gtgaagcgca
     2821 tcaacgacgc cgcgcgcggc aaatggctct ctgtggtcgg catggcgcta tcggtagcgc
     2881 gcaattacga cccgttccag tccccgaccc acttcaagct gtcggacatg atgaagaagt
     2941 tcgataagac cttcggagcc accggcaaga gctacgggcg ggtagatgcg acgcggaccc
     3001 gcgaggacat cgaacgccgg cgctccgatc gctggaactt cctgatgatc gtgggaatgt
     3061 ggttccagga tctcttcaac tacgatttcc gccgcacgga gcagtgcatc atcccctatg
     3121 ccacacagca gggtgagatt tccttctgcg cttacaacac tggtatgggc tggcggaaca
     3181 tcgttgagaa gatgcacatg accgccacgc tcaccaagtg gtacgaggag catggcaagc
     3241 acgagatctt cgccggcggg aagagtgtgg ctcttgagga gaatgcgcac tcgctcatgc
     3301 tgcgcgacga acttgtgacg aaggaagagc agcgcgatct cgatcttctt ggcatcgcca
     3361 agaccgcgcg ggaagagaag atccgcgccc gcgacgaaaa aatgcgcgag gcacagaaga
     3421 atgctgagat ggcgaaactg taccgtcagc acgtattgaa agagcagcct ggtccggatc
     3481 tggtccagat cggtggaatt caacccgcac cttccaacgg tgccctcaaa gaggagcgcg
     3541 aggaagttgg cagcttcggc gactgatctt cgccggaagt acctttaaac actggccggt
     3601 ttctattcgt gccgcagcgc agtcgccgga tcgatccggg acgcattccg ggcgggtaga
     3661 tagccggcca gaatcgccgc acttatcagg atcacgaccg atgctgtgag tgccagcgga
     3721 tcgttcggtt tcattccgaa gagaaacgac tccaccactt tcgacgccgc aagagcggcc
     3781 ggcaaactga tcgcaagtcc caccgccgcc accacacaaa cttcgcggag aaccatccag
     3841 accacccgcc cacgttgcgc tccgagcgcc atccggatac cgatttcgcc ggtccggcgg
     3901 gcgacgttgt aggacatgga gccataaagc ccgacgcagg cgatcgccag cgcgagcagt
     3961 gcgaaagcgc tgcataagcg tgtaaatgtg atctcctggt tgatggtccg gtcgatcaag
     4021 gcgctttggg ttttcacctc ggacagcggg atgcggttgt cggcccggcg cacgatatcc
     4081 cgcacggctc cggtatatcc aagcggattc cccgtagtcc gcagcgcgta gtgcatctca
     4141 cgcaccggcc cccaggcgcc ttgtgcaaat ggaagataca cgatgggcgg cacgtctccc
     4201 ttaaggtttc catagagcga gtttccggag acgccggcga tttcgatatc gcacttcggg
     4261 cacatgcgcg gcaaactcag gcgttgtccc agcggattcc ggtcgccgaa gctcagcttg
     4321 gcaaatttct cgttgaccac agcaaccatg ggcgagcctt gccggtcgcg ctcttcgatc
     4381 tcacggccca acaggatcgg aatctgcatc gtgtgaaaga agccggttcc gatcaataaa
     4441 attcggctgt tctggatcgc gccgccgggc aggccgacat tcgttgcgct ccagccggtt
     4501 ccgatcaaca tgtgattcga caggctcgcg gcgcggacgc ccggaatggc gccgaactgt
     4561 ctctgaagat cgttataaaa agtgacgatc ccgggatcgt cgtgtccggc ctggcgcgcg
     4621 ttcaactgga acgtcagcag attttcgcgg ttgaatccga gttgaatgga tgtcagattc
     4681 gaaagggttc tcgcaaacag cccggctgcg accaggatga agaggctgat ggcgatttgc
     4741 gagaccatca gaatgcggct caggctcagg cggcgaaatc cgtgcgcgag cacttcgccg
     4801 gtgcgggatt gcttgagtgc gggcatcagg cctacgcgcg tcgcctggag cgcgggagca
     4861 agcccgaaca gcacacctgt caacagcgat agtccggcca ccacgcccag cacatgccag
     4921 ttcaaaccgg cgcgcaaggt gaaatcctct tgtccattcg ccagcagcaa agtaagaaaa
     4981 cggattcccc acaccgcgaa cccgacgcca agcgcaccgc ctgccacggc aagcaggatg
     5041 ctctcggtca gaagttggcg tatgacgcga aagcggccgg cgcccatgct cagccgcacg
     5101 gcgatttccc gcttgcgcgc ggccgctcgc gccagaagaa gattggcgat attcgcacag
     5161 gcgatagcga ggatgagtcc taccagtgtg agcaggatat acaacggctt cgagtatctg
     5221 cgccgcaggc tgtcgagtcc gcctgcgcct tcccttaaaa ccagcgtggg aacgtcctcc
     5281 ggcttcagat ttggcttggc agcacgcccc cattcgaaga acggagtagc caacgttgcc
     5341 tgggcttgca ccatagtgac gcccggcctc aaacgcgcca tgggcacgac ccagtcgtaa
     5401 ctaggattgg cgtagatccc ggtgggagga tagcgcttgt ccccggcttc cagaagcagg
     5461 ttggcgtgca tcggaatata gatgccggga gccacgtccg gatcggcgcc gaagaactcg
     5521 ggcggagtga caccgatcac ggtgaagggc aggttgttga tcagaaccgg ttgccccggc
     5581 gcgttttcgg gacctccaaa gcgcctttga ctcaatgcga agctgatgac ggcgaccgcc
     5641 gcggagccgg cgcgatcatc atccggagcg atcaggcgtc ccgcggccgg cggaacgcca
     5701 agaccacgga aatagtcccc ggacacgtat tccgtaacta cgagctcagc ctggccgcga
     5761 aacgtgaggt tgagtttacc ggcgccttga tatccgaaga cgctcgaaaa aacagtattg
     5821 ttctttttga gaaattcaaa ggccggatag gagaagaagc cgccgatgaa tcctccattc
     5881 ggatcggcgt aactattgtg atgccggttc gtaccgcgtg tttcgtcgcg aggcgtatgc
     5941 caactgagaa tcaccaggga ctgcggatcg gagaccggca gcgagcgcag cagaatcgag
     6001 tccaggaaac tgaagatggc ggtgttggcc ccgattccca gtgcgagcga caggattgcg
     6061 agcgcgctga aggttttgtt ggccgtcatc gtgcgcagag cgtagcggac gtcctggacg
     6121 agttgctccc agaagatcca cgtccacgcg gctcgcgtat cctcctgtac taaagcgata
     6181 tttcccaggt cccgccgggc cgcccggcgc gccgcttcgc tcgccagccc acgctcttca
     6241 agctcgccgg cttcctcttc gaggtgaaac tggagctcct cttgaagttc cgcttccttg
     6301 cttgagcgcc gggccagcca cttcagtttg ctgaaaaagg aggtcattgg ctttcctggg
     6361 gcaccggctt gacaatcaat ccaatggcgc gcgcgagcgc ttcccatttg gattgttcgt
     6421 tggcaagttg ttttcgtccg gcggcggtga ggcgatagta ccgggcgcgc ttgcctttgt
     6481 cggagagatt ccaggaagcc gcgatccagc ccttcgcctc cagccggtgc agagccggat
     6541 aaagcgagcc ggtctcgacc tgtagcagat cctctgaagt ccgctgaata tgcttggcga
     6601 tcgcgtgacc atgattgggt cctgccatca gtgtgctcag gatcagcaga tcgagggtgc
     6661 cttgcagcag ttctacgcgt tcgggttctt tgcgcttcat tatgtaggta ttctactaat
     6721 atttatgtag gctgtctacg aaataatcgg cgggagggga gggaagtcct gagatatgct
     6781 ggaagcggtc taaccaatgc atccgctgct caaatcgctg acgggtggcg accggcgctc
     6841 aatcggtaag tcgaaccatg tggcttccct cgtcctggag caaccggaat tgatcgaggt
     6901 attggttgag ggaatggaga gtgcggatcc ggtgctccgg acgcgctcgg cggatgccat
     6961 ggaaaaggtt accgcggtcc gccccgagct tcttgtcccc tacaagaaaa agctgctgca
     7021 acaattttcc aggatcgagc agcaagaggt tcgatggcat gttgcgccga tgcttgctcg
     7081 tctgccgcta tccgaagtgg aagaaacagc cgtcctgaac attttgctaa gttatacgaa
     7141 cgatcgcagc agcattgtaa aaacgatggc catgcaagcc ttggctgaca tcgcgctccg
     7201 cagccttcga ttcctgccgg aagtgcaacg gcacatcgag gagcttaccg cgaccggcac
     7261 ccccgcaatg aaagcccgcg gcaagaaact gctgaagcaa ctcgcgaaac cgttgcggcc
     7321 agccgcgggc ggtgagtcct cccacggttc ctgccgcccg tagacggtgt gcatctgccc
     7381 acctggcgtc cggccaggag ggtgtcgagc gagttctggt gttaaacacg acgccgcttc
     7441 tgtttgcgta tcccgataac cactccgaaa ccagtaccga gccacgaccg ccagggagcg
     7501 gatgcgcaat catttctgac gccacgttta gatgccgaga tcggtcaagc cggctggagt
     7561 gacaaggtac gcttgatttg tttcacccgg tctgaaacgg agcctgaatc gatcgggata
     7621 atctcaaact cgagatcccg ataggtccgt tcgtgaatct gttcgaagag gattgtttct
     7681 tcataagtga tccttcgcgc ctcagtaggt gtgacgaatc ctaaattctt tagaaacaaa
     7741 actctgttct gaaacacgtt ttccgtcctg acccgtctca gttcttgcag caggttttga
     7801 ggcttggaga atccaagata gtccgccagg gcagcggtac acaccacgga tcggtcgtga
     7861 aactgcacga gatctgtggc gcagacggcc cggcgctctc tggtttgttg aaggctgaca
     7921 accgcgttaa tgaattccgg acgggcccac ggctcggcga tccccttcgc ctgccacaaa
     7981 gcaataacgt ccgttgccgc ttcctcgaca acgctaaacc cgtcaatttc aagttgccga
     8041 atgattgccg tcttgcccgc gccaggcgcg ccagtcacca catatctctt cataacggtc
     8101 tgccttgcct ccgcgacgaa tctttcgcta ccctggtaac ttgctggata ttgttcgcta
     8161 acaccaactg acgcggacag ctcattccct cgagctgaag gtagtggtgt ttctttatat
     8221 agatcctgga gcgaacaaat ccatgtttct cgaacgtctc ggcgccgatc agactgtcta
     8281 tggcgctttc tctccttacg cccggcagca aatggtgctg gcccgcgtgg ctgtcgcaca
     8341 gcgcgatgtc taccatcttt atacggaaga cggcgccgcc cacgcagagc catcaggaag
     8401 gctttattac catacgcccg gccactcctc tttccccgtg accggcgatt gggttgccgc
     8461 gcgcatcgtc ggccccgaac aggcgctggt ggaagccgtc ctgccgcggc ggacctgcct
     8521 ttcccggcgc gcggcgggcc gtcgcgagga ggaacaaccc ctggctgcca acgtggacgt
     8581 ggtctttctg gtttgcgggc tcgacggtga tttcaatctt cgccggctgg aacggtacct
     8641 ggcactggtg gccacaagcg gcgcttcgcc tgttgttgtg ctgaacaaag ccgatctgtg
     8701 tgccgatcct gcaggccgaa tcgccgatac ggccgcgatc gctcgccatg tgccggtcgt
     8761 tgcggcaagc acggtggatc cgcacgggct cgacgctctc cgccggttcc tcacacccgg
     8821 agggaccgta gcgttgctcg ggtcttccgg tgtcggcaag tcctccattg ccaaccgcct
     8881 gcttggcgag gagcgttttc ggaccggtga agtgcgaatg caggactctc gtggacgcca
     8941 cattaccgtg cgccgggaac tcactccact cccgcaaaca ggcgcgctca tcgatacgcc
     9001 cggaatgcgt gagctgcaac tgtggacagg gccggagagc atcgatgccg tgtttgacga
     9061 catcacggcc atcgcccaaa actgccggtt ccgcgattgt acccactccg gcgagccggg
     9121 atgcgcggta gccgcggcgc tggatcgagg gcaggtggac acggaccgtt gggcgagttt
     9181 ccaaaagtta cgcgcggaag cgggatggca cgaggccatg tcagacccac tcaccgccct
     9241 ggaacggaaa cggaagtgga aaatcatcca taagcaggca cgtgccttca ataagtcgcg
     9301 ccgctgatgc tttgctacac cgccatggcg ccgatttgcc gaagcagagc aaggttgtcg
     9361 aacatggacc aatgctcaac gaagcgccca cctaccacgc ggttgaattc aaggcctgtg
     9421 aacctcacag gccgccccgt aggcggaatc ccctggaaca tctcctggtg cgtgccggtg
     9481 agtgtccagc gagtgaccac cgtgtcccct tccgcgatgc aggactcgat tgcgatccgc
     9541 gaatccggga aaccttcggt gaaagcggcc atgaatctcg accatgcgtc attgtccagc
     9601 ggctctgcgg cgcccgatag gtaggcccgg aaactctcgc cgcgcacggc cctctcggtc
     9661 cgccagtcgc gttcgttgaa cgcctggatc caacgatgat tcaagctctt caggtcggcc
     9721 ggcggatttg cttccccttc gaaactcttt ggttccggcg gcaacggtcc gtgaatttcg
     9781 attccatact tcgcggccaa catcatcagc ttgtccatat ccggcgagga aggcgggtct
     9841 gtcatcggcg tcgtttgttc gaggacaaat ccttcaaatc cgccaggggt gcacattacc
     9901 agcattctcg ccggagagtg gccgatgacc ttgaatccgt gcgggacctc gcgcggtcca
     9961 tagacgaatg tgcccggacc agccttcaac cacttcccgt cg
//
LOCUS       DASTMK010000565        17616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_605232, whole genome shotgun sequence.
ACCESSION   DASTMK010000565 DASTMK010000000
VERSION     DASTMK010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17616
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605232"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VFQ79_23230"
     CDS             complement(<1..1094)
                     /locus_tag="VFQ79_23230"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142655.1"
                     /translation="MRFSLIVLSSVLLLAASIPLELSAQSITGTIVGTVTDVSGGTVS
                     AATVTLTNEDTNLEYRAAVAATGEYVAPNLPPGTYTVKTELAGFKPSVIRNLRLLASR
                     TVRADVVLEPGAVAQAIEVSASAPVITSENATIGNVLESQTIRTLPLNGRTLDRLIRI
                     SAGVTSDSASNPRVAGSAYWGGIQFSVDGVTYNDMGNGGGAYSYRNGLATLPSVDSIS
                     EFKMDSNNQKAEYEGSVSATVVTKSGTNELHGSVLWFNRNKEYAAKNFFATGLPNPPF
                     NRNEFGYSVGGPIIKNKTFFFHSYEGLRERTARTNTLSVATQAMRSGNFAGLPVIIDP
                     LSGLPFDGNQIPASRIDPRSKALIDKVPLP"
     gene            complement(1245..2888)
                     /locus_tag="VFQ79_23235"
     CDS             complement(1245..2888)
                     /locus_tag="VFQ79_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142656.1"
                     /translation="MREIRRLTLNRLALLLLLASAPLSAGMVRFKERCLEDLVAQVPK
                     ILASQDAKTGRFGEGIWIVTDQNVLLPLAAAWSFQHERNPYYHKPEILNAIMSGGDAL
                     IDDQDADGRWVFRKKDASTWGKIYMPWTYSRWIRAFELVRGAMPEARRSRWDRALRLG
                     YESIAKELARARIHNIPAHHAMGLYFAGRVFDRPEWKQQASEFLHRVAQEQQAPGYWS
                     ENKGPVVLYGTVYVEALGTYFAASRDAAILPVLCKSGVFHSYFTYPDGSDVETIDERN
                     YYHPDVRLPNVGFTVTPEGRRFTNRQLLAFKGTLTADLAASLLLWGEEGEEGGGGAPE
                     GDFDYVLGDGEAAVRRRGPWYLVVSAVTAPVPASRWIQDRQNFISVYHDAAGLILGGG
                     NTKLQPAWSAFTIGDTGMWLRPQGEEEPDFRPPSGLVHVPTSAKLLTGDLFGVELDYA
                     GRKGRITLDVKGPGRLEYVVSGDSSMTAHIVALRGLGDGRPSKPEVRLQPPAGSILRY
                     PMLPYDPYRKDGKAGAEHGRLVIEFSAGGKQTTVVEVRH"
     gene            2970..4205
                     /locus_tag="VFQ79_23240"
     CDS             2970..4205
                     /locus_tag="VFQ79_23240"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HEU0142657.1"
                     /translation="MRKFLLALLSVSAAAQSPQDWLLPNFAERLELDAVNRSAEHAST
                     LAVIPVPEASQVALRFPGSLSIVVNPEDRAILPSQVDDVDGNGTPDEFVFPVELGPGE
                     TKRFHIYYSTTLRDHLPWPKQVHASHAFGYNRATVALESESIGYRTYGGLFLDIQARL
                     KDRPGLYNSLVGYFGSGRPSAAGLDIIHLGDTLGLGGLFLRAGDDVHRPPLNMPDYAH
                     KPPVPEAPEYRVIAAGPLRAVVEAQMNRWRIGADEVAVKAIYSIAAGKSHVDCRFEIV
                     PLRLSRTYEAGAGIRHLPSMKLDSTKGRISLMGTQSPKTGPLGMALYYNPAEADTAAP
                     LATKEDRNEIIVFREKLEPGRAMKGRYSVAAAWSGSGIDDLLGHLAGIEAQARASVRL
                     ENFRVSRTPRPERLEGEAY"
     gene            4205..4942
                     /locus_tag="VFQ79_23245"
     CDS             4205..4942
                     /locus_tag="VFQ79_23245"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0142658.1"
                     /translation="MIADGHVHIGKSTRLQINADGEMLVRIADELGIEKICCTDLTAL
                     FYDMHEGNRLLFEQMKLFPDRILGWASLHSTRFGREALDELERCRSDYGMRGLKIYST
                     PDMSIAEPAMIPILEKCVELDFPILGHTTPQECEYLLGYVPECKLIMAHAGGQPFAKG
                     DWNRAIMSAQRFENLYLDTASSTIDCGFLEACVAALGPERIIFGTDTPLLDPWAQLAK
                     IRETNLSQPDLDLILGGNVLRLMGVAA"
     gene            4939..5706
                     /locus_tag="VFQ79_23250"
     CDS             4939..5706
                     /locus_tag="VFQ79_23250"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0142659.1"
                     /translation="MIVDINAFIGKWPYWPIAATSPAEVSAQLAGWRIDRAAVCSTRS
                     VFVDWQDGNCEVEQASHAESRFIPFACLGTLELSHALPRTGYDFEGYAARGFRGIRLY
                     PQHHSYHPLFNSFTGNILEQAEMRQWPVLLPLRIIMNWGMPMLELGVIEALVERHPRV
                     IWILAGINYLHELELAVTLLRRNANVHLETSCVMGYHAIAKLVEQCGSEQILFGSGAP
                     VQHGAGVAKIAKAKISEADRERLLEKNALRLLRLEEK"
     gene            5703..6563
                     /locus_tag="VFQ79_23255"
     CDS             5703..6563
                     /locus_tag="VFQ79_23255"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0142660.1"
                     /translation="MRILRFFSDDGSVHLGVRDGEWVTDLGASSPISLMDGKPPTSGP
                     RVPYAGLRLDAPIRDCPKLLALAGNYREHVREAGIEDLAPSANITPQVFWKPSTTGIN
                     RPGGVIPIRPNNVFVDWEIELAVVIGRWTKNVKAEAAMESVFGYTILNDISERKFNSL
                     LEGRHVRQFDPFFDWLMGKWFDGHAPLGPEIVTADEVPDPHNLGIRLSVNGELMQDSN
                     TRHMIFRIPETIAYIASVLTLEPGDIIATGTPHGVGSARGICLKPGDVIRGEIEGLGI
                     LENVIALEES"
     gene            6560..7603
                     /locus_tag="VFQ79_23260"
     CDS             6560..7603
                     /locus_tag="VFQ79_23260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142661.1"
                     /translation="MKDKVRIGIIGSGFMGRTNAETVTRYLERAELVSVAGGSRAPAL
                     AAEYGVTSAASVDALLARDDIDAVLISTPHAEHCSQAVAAAQRGKHILLDKPMAVTVE
                     ECDRILEAVRAARVKLMIMFGQRFRICNIEARRLIREGAIGKVKMILELILASGGLQT
                     LPPWQSLAENYGPFLGHAVHNLDRIRWLTGAEFTSVSAQVQRDAATGNEVSTMALYGL
                     SGGAAATVWESWDLAPPAFPRSASGAWIVGETGNLDLDAYGQLRLGKNKEWTVVAEQP
                     PIDWQGQGMLSPVRMQAYRDQHQEFINSILEDREPSVTGADGRAAVEAAQAAYRSEAE
                     GITIHLRAKETAQ"
     gene            7600..8862
                     /locus_tag="VFQ79_23265"
     CDS             7600..8862
                     /locus_tag="VFQ79_23265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0142662.1"
                     /translation="MIEQLAVDGGPRAVEEKLPTFLESAGRSFGVEEESLVLEALRNG
                     CLSRNGGTMVLQFEQEFAQALGVPHAVACSSGTASIHLAVAALNLEPGDEVIVPPITD
                     IGTVLPVLWQNAVPVFADVDPRTMCLDPADVAGRISGRTRAVIAVHLAGQPCDMDALA
                     RICRERSLVLIEDCSQSYWAEHEGRLAGTIGDMACFSLQQSKHMTCGEGGILVTPRED
                     YARRARLFTDKAWPRETKSLGSCRFLFLAQNYRMSELAGAVALAQLAKVRDTVARRRA
                     SAERLTALLAEVPGVTPPHVPSGTLHSYWLYMLRVNEAEAGVTARRFGEALTAEGVPA
                     WVEYIIDPLYLSPIFTESQTYGTSGYPLREFGRQNYQRGLCPNAEAVLKSIIAIWWNE
                     NYTEAHVHQISDAIRKVGSHFSLAGARQ"
     gene            8873..9694
                     /locus_tag="VFQ79_23270"
     CDS             8873..9694
                     /locus_tag="VFQ79_23270"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0142663.1"
                     /translation="MPKSDIYYIEVLGKALDILDVFVEVRKPRLTLQEISTLTKLNKN
                     TVFRALYTLAEHGYIIKRHHEYELGSKLLDLSNAKLQRRDLVAAAGPYMDSLREKFGE
                     TVNLGVLDGGAIRYVDVRESPHRFRLAERVGGSDFLHCTALGKCHLAFMPMEEVRRLL
                     KEQGMPRETPRTIVTLTAMKAELERVRRQGYAADPEESMEGAFCLGAPILDDTGAPIA
                     ALSISGPTTRFNASVTPEASKALLDAVSEIRARLGYTRRQEVLQEQIRNKAATGS"
     gene            9729..10874
                     /gene="menC"
                     /locus_tag="VFQ79_23275"
     CDS             9729..10874
                     /gene="menC"
                     /locus_tag="VFQ79_23275"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEU0142664.1"
                     /translation="MVSIEELAKESGPLRFTGFDLYRIAIPMREPFRISSGEVAVKDS
                     LLLKINGGDAFGWGESSAMPGAFYSSETPDSCWEDLSTAVLPALASRWFPDMVSLEEF
                     LKERSQSAFVRVAVETAAWELLARRKGISLRSLFSIADRDVVSGLAVGLYDTREELRE
                     ALERYRPRDYARLKIKIKRGQDVELVRAVREWFPDVRLFVDANADYGREDFATFQELD
                     AFGLMMFEQPLARSEFEASAELQRLVRTPVCLDESIETADDARRAIQLGSCRIVNIKL
                     QRVGGYLEALRIIEICSRSGIPVWMGTMPELGIGSAQALQLAAHPAFRFPTDVEPSER
                     WYEDDILTPRLSLANTRLPIPAGPGIGFQVDERKLEQYCTARKTFKT"
     gene            10878..12611
                     /locus_tag="VFQ79_23280"
     CDS             10878..12611
                     /locus_tag="VFQ79_23280"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142665.1"
                     /translation="MEHMHLAAADYLVVGGYFVIVLWIGFYFRNRLEAAKDYFAGGNQ
                     IPWWLAGISHYMSSFSAFAFIAYAQIGYSHGWIAVSLFWATIPGCLIGGLYFAQRWRR
                     ARIITPIQFLEARFNGFVRQLFAWSGIPMKIFDDALKVFATGLFVSVAAGLNLHWSIA
                     VCGLVMVAYTFLGGLWALVVTDYVQFLMKALAILLLLPLAVVKAGGWQRSFSALPDGF
                     LQPMGGPFNWTYLAGFTILMMISYNASWSLAQKYYSVRNEKEAAKVAYCAGALNLIGA
                     PLMILPALIGRNFLPNLVEQNRTADVYVLLVMELLPVGMIGIIMAAMFSATMAMVSAD
                     FNAIASVLTKDVYHRLIRPEASEKHLLNAGRWITLALGLLTTLLALWVAAFRQQSLFN
                     TMVTVLGLFMAPTFLPLLAGLAIRKLTWQGAFAGFLCGLVTGFAMLAVKTWWLPPASD
                     AGSSYFFEGISLLANTAATILGMFAGTVLSRSSAGRERKTQQFFRELDRPIAPEEVPA
                     RSSNPAASVLGLSTIGVGALLFLAGAVSGSATARVIDFAVGAVLIVIGVFFRRRAGQS
                     SGKPAAVVTTS"
     gene            12627..15857
                     /locus_tag="VFQ79_23285"
     CDS             12627..15857
                     /locus_tag="VFQ79_23285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0142666.1"
                     /translation="MSTSKKALTALLMFSAATFTYAQSTDATILGVVRDPSGAVIGGV
                     EVIATNVRTNVAKRVFSNDSGNYEIPALQPSEYRLEATSKGFKKQVLTGIILQVNQAA
                     RLDIVMQLGDVVEAVTVEATALLIQTDTGAVGQVIDNRKIVDLPLNGRNFTQLATLTP
                     GVTPSGAMGTGRAQSVFVSGSRATKTEFVLDGVSSTGPINGGTGILPSIDALEEFKVQ
                     TSAFAAEFGRSPALVNISIKSGSNAFHGSAFEFVRNDVFDARNTFALSNPPLKRNQFG
                     VTFGGPLKQNDFFFFGNYEGLRQRRGATFNAVVPTAAQKNGDFGSIAIFDPATTRLEG
                     STYVRSPFPGNRIPADRIAPEAAYFLKFFPEGNNSDGARYIVSPSNSDDNDQFTIRLD
                     KKVKSAGLLFGRYTFSNVKTFSPSAFPALVGVMLRSRFQNAGVTYTHTWRPTLISETR
                     LGYNREYTLEDAPGIGTNHTVLSGITGFDRTSESVPRFPQIGISGFTGIDGRTFRPLN
                     NTNDIYQIIQTVSWYTGKHTLKVGADLRQQRNTNWNTAYNSGNFSFSGTYTLNPGVAR
                     SGSGFADFLLGLPASANRSFPRDAFGNRFGNYHFFAQDDWRATQNLTINLGLRYEFNP
                     WPLGYRNQLTLFDRSLGKVIISDPINFEAQKITRAAYDAMPDAYVTTKELGLPQQIQS
                     NDLNNFAPRVGFAWRVLGDNRTVLRGGYGVFYELVNGNGRTGGVINPPFLFDEVANNN
                     NPIPNRTLHDFFFTQPPSAASPPIIDSRPLNQRTPYEQTWNLMIQRELFANMALEVGY
                     LGKKGTHLERDVLFNQPEFPGPGSIQSRRPFPRFGSGTLRDDGSNSTYHGLQTKLERR
                     FSNGLSFLAAYTWAKSMDDVSTDLGGAVQNWKDYRAEHAVSDFDVAHNFVFSGLYELP
                     FGPGKPVASGAGGALSRLIGGWQLGAIVQQRSGLPFTPRISLDQANTGTSQRPNRLGT
                     GTVDQRTTARWFNPADFTIPAQYTFGNSGRNILRADRYNNIDLVLLKNTTITERVSTQ
                     LRFEFFNAFNHANYGAPNAVVNTATAGQVASANDPRILQFGLKVVF"
     gene            15907..17232
                     /locus_tag="VFQ79_23290"
     CDS             15907..17232
                     /locus_tag="VFQ79_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142667.1"
                     /translation="MNLLKGFYRAILGTGLLAAAVSAAELRVAAFRADATPAMGEPLI
                     WIQPTTKVEDPLLAKGVVLDDGKHRYVLCSVDWCGLGGSTHLLFRRKAAAAAGTDISR
                     VTIHSVHQHTAPYIDGGAYSLMRTFPNPPLLMSDEYLEAVTDRLAGAVKEAVKRLQPF
                     DSIGVGEARVERVASARRIHDDGKLVTRYSTGAKDPKMAALPEGEIDPAVRTVTLANG
                     GKALVRLHYYATHPQTFCCDGRVSGDFVSTAREKLEQDEGIPQVYFTGCAGNVTVGKY
                     NDGSEKARAELARQLRKGFDSSIDATRFHKAAALRWKTTDLKLPPKPGVPTDPSALKG
                     KSGVDLYRSAQATAFCMRAEPLTMSLLEIGPARIIHMPGEPMLEFQKYALGVNRSPFL
                     AVAGYGDISPGYLCTDQAFREGGYEPSASNAGPGTEAQIKKAIDKLLEK"
     gene            17249..>17616
                     /locus_tag="VFQ79_23295"
     CDS             17249..>17616
                     /locus_tag="VFQ79_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142668.1"
                     /translation="MSSSHKNSGPGETRRSFVQTGGAALAAMGAGTAAFGKTETLALN
                     GGAKSVSFPTAKESALTKWPRYGATEKKVLHELIDSSKFYEELPLFENEWKAYTKSPF
                     VKAHMNGSSALTSMYFALDLP"
BASE COUNT         3534 a         5241 c         5384 g         3457 t
ORIGIN      
        1 ttgggcagcg ggactttgtc gatcagggct ttcgatcttg gatcgatccg ggacgccggg
       61 atctggtttc cgtcgaacgg aagtccggac aacgggtcga tgatcaccgg cagtccggcg
      121 aaattgccgc tgcgcatcgc ctgggtggct acggaaaggg tgtttgtccg ggccgtccgc
      181 tcgcgcaacc cttcatagct gtggaagaag aacgtcttgt tctttatgat gggtccgccg
      241 acggagtagc cgaattcgtt ccggttgaag ggcgggtttg gcaggccggt cgcgaaaaag
      301 ttctttgccg catactcctt gttgcgattg aaccagagca cggagccgtg cagctcgttg
      361 gtaccgctct tggtgaccac cgttgccgat accgaccctt cgtactcggc cttctgatta
      421 ttcgagtcca tcttgaactc actgatggag tcgacggatg gcagcgtggc gagcccgttg
      481 cgataggagt aggcgccgcc gccgtttccc atgtcgttat atgtgacacc gtctacacta
      541 aactgaattc cgccccagta ggcgcttccc gctacacgcg gatttgaggc gctgtcggag
      601 gtcacgcccg ccgaaatgcg aatcaggcgg tcgagtgtgc ggccgttcaa cggcagcgtc
      661 cgtatcgtct ggctctccag aacgttgccg atggtggcat tctcggacgt gatgaccggc
      721 gccgacgcgc tgacttcaat cgcttgcgcg accgcaccgg gttccagcac gacatccgcg
      781 cgcaccgtcc ggcttgcaag caggcgcagg ttccggatga cgctcggttt gaagcccgcc
      841 agctcggtct ttacggtgta agtgccgggc ggaaggttgg gagccacata ttcgcctgtt
      901 gcggcgacag cggccctata ttccagattc gtatcttcat tcgtcaacgt tacggttgcc
      961 gcgctgactg tgcccccgga gacatccgtc accgttccga cgatggttcc cgtgatgctc
     1021 tgcgcggata attctaacgg aatcgaggcc gctaacagca ggacactcga caagacaatg
     1081 agtgaaaatc gcatgcgaag ctcctcccat acttgagact tgactgaacc tctgcaacta
     1141 cctcttcgtt taccctgaat accagttgcc tgaatggtgc ccgataagct aatgcgctca
     1201 taataacgcg gcgcccacca ggaatgcagc aggagacggc cctgtcaatg ccgcacctcg
     1261 accacggtgg tctgcttccc tcccgccgaa aattcgatga ccagcctccc gtgttcggca
     1321 ccggcctttc cgtccttgcg gtaaggatcg tagggaagca tcgggtaacg gagaatgctt
     1381 cccgcgggtg gctgtagtcg cacttcgggt tttgacgggc ggccatcccc aaggcctctc
     1441 aacgccacga tgtgggccgt catcgacgaa tcgcccgaaa ccacatactc gagccgtccg
     1501 ggtcctttga cgtccaacgt gatccgtccc ttgcgcccgg cgtagtcgag ctccacacca
     1561 aacaagtccc cggtgagcaa cttcgcagac gtgggcacgt gaaccagtcc ggagggcggc
     1621 cgaaagtccg gctcttcttc gccctgagga cgcaaccaca ttcctgtatc accaatggtg
     1681 aacgcgctcc aagcgggctg caacttggta ttgccgccgc ctaggatcaa acctgcggca
     1741 tcgtggtata cgctgatgaa gttctggcgg tcctgaatcc atctcgaagc cggcacggga
     1801 gctgtgactg ccgacacgac gagataccag gggccgcggc ggcgcaccgc tgcttcgccg
     1861 tcgcctaaca cgtaatcgaa atcgccttcg ggcgctccgc cgccgccttc ttctccttcc
     1921 tccccccaga gcagcaacga cgcagccaag tctgccgtta gcgtaccctt aaacgcgagc
     1981 aactggcgat tggtgaaacg gcgcccctcg ggagtgacgg tgaatcccac attcggcagc
     2041 ctcacatcag ggtggtaata gttccgctcg tcgatagttt ccacatccga gccatcggga
     2101 tacgtgaaat aggagtggaa cacgccggac ttgcacagta ccggcaatat agcggcgtcg
     2161 cgcgatgcgg cgaagtaggt gcctaaagct tccacataaa ccgtgccgta gagcactacg
     2221 ggccccttgt tctctgacca gtatcccggc gcctgctgct cctgggccac cctgtgcaaa
     2281 aactcggatg cctgctgttt ccattccgga cgatcaaaaa cgcggcctgc aaaatatagt
     2341 cccatcgcgt gatgggcggg aatgttgtgg atccgcgcgc gcgccagctc tttggcgata
     2401 ctctcgtagc cgagccgcag cgcccgatcc caccggctcc tcctggcttc gggcatcgcg
     2461 ccgcgaacca gctcgaaagc gcggatccag cgcgagtagg tccagggcat gtatatcttg
     2521 ccccaggtgg aagcgtcctt cttacggaag acccaccggc cgtcggcgtc ctgatcgtcg
     2581 atcagtgcgt ctcctccaga catgatggca ttcagaattt ccggcttgtg gtaatacgga
     2641 ttgcgctcat gctggaagga ccaggcggcg gccagcggca acaggacatt ctgatccgtg
     2701 acaatccaga tgccctcgcc gaaccggccg gttttcgcat cctgcgaagc gaggatcttc
     2761 ggaacctggg caacaaggtc ttccaggcat cgctccttga aacgcaccat tcccgcgctt
     2821 aaaggcgcgg aggcgagaag cagcaacagc gccagtctat ttaacgtcag tcggcggatt
     2881 tcgcgcattg gtttgctccc atcacgtgcg gcgagacgct tggcatcgat agcttgtatg
     2941 gattatactc ttcaaaacgg cgtttgagaa tgagaaaatt tcttctcgcc ctgctctcgg
     3001 tcagcgcggc ggcacaatct ccgcaggact ggctgcttcc aaacttcgcc gagcgcctgg
     3061 aactcgatgc cgttaacaga tcggcggagc atgccagtac gctggcagta ataccagtac
     3121 cggaggcttc gcaggtcgct ttgcggtttc ccggatcgct ctccatcgta gtgaaccccg
     3181 aagaccgggc gattctgccg tcgcaagtgg atgacgtcga cgggaatggc actccagatg
     3241 aattcgtttt ccctgtcgag ctgggtccgg gggaaacgaa gcggttccac atctattact
     3301 cgaccacgct tcgcgatcac ctcccgtggc ccaagcaagt acatgccagc cacgcgttcg
     3361 gctacaatcg cgccacggtt gcgctggaat ctgagagcat cggctacagg acatatggcg
     3421 gcctgttcct ggatattcag gcgcggctaa aagaccggcc cggtttgtac aacagcctgg
     3481 tgggatattt tggctccggc agaccgtcgg ccgccgggct cgacatcatt catttgggag
     3541 acacgctggg gttgggaggc ctcttcctac gcgcgggcga tgacgttcac cggccaccgc
     3601 tgaacatgcc cgactacgcg cacaagcccc cggttccgga agcgccggag tatcgcgtga
     3661 tcgcggcggg tccgctgcgc gccgtggtgg aagcgcaaat gaaccggtgg cgcatcgggg
     3721 ccgacgaggt agctgtgaaa gccatctatt ccatagcggc cggaaagtct catgtcgatt
     3781 gccgtttcga gatcgttccg ctccgcctgt cgcgcaccta cgaggcaggc gccggcatac
     3841 ggcatctgcc ttccatgaag ctcgattcca caaagggccg catctctctg atgggaacgc
     3901 agtcgcccaa gacaggcccg ctcggaatgg ccctttacta caatccggcg gaagcggata
     3961 cggccgcacc gctcgcgacg aaagaggacc ggaacgagat catcgtgttc cgcgagaagc
     4021 tggagccggg acgcgccatg aaaggccgtt actccgtcgc ggccgcatgg agcggcagcg
     4081 gcatcgacga cttgcttggg catttggcgg gaatcgaagc gcaggcgcgg gcttccgtcc
     4141 ggttggaaaa tttccgggta tccaggacgc cgcggccgga gcggctggaa ggtgaggcgt
     4201 attgatgatc gccgacggcc atgtgcatat cggcaagtcc acgcggctcc agatcaacgc
     4261 ggacggcgag atgctggtgc gcatcgccga cgaactcggt atcgaaaaaa tctgctgcac
     4321 ggatttgacg gcgctgttct acgacatgca cgaaggcaac cggcttctgt tcgagcagat
     4381 gaagctgttt ccggatcgca ttcttggctg ggccagcctg cattccacgc ggttcggccg
     4441 cgaggcgttg gacgaattgg agcgatgccg gagcgactac ggcatgcgcg gcctcaagat
     4501 ctattcgacg cccgacatga gcatcgccga accggcgatg attccgatcc tcgagaaatg
     4561 cgtcgagctt gatttcccca tcctgggcca caccacgccg caggagtgcg aatacctgct
     4621 gggctacgtg ccggagtgca aactgatcat ggcgcatgcc ggaggacagc cgtttgccaa
     4681 aggagattgg aaccgcgcga tcatgtccgc gcagagattc gaaaatctct acctcgatac
     4741 ggccagctcc accatcgact gcggatttct ggaagcctgc gtcgcagcgc tcggccccga
     4801 acgcatcatc ttcgggacgg atacgccgct gctcgaccca tgggcgcagc tcgccaagat
     4861 ccgcgagacg aacctttcac aaccggacct ggatcttatt ctcggcggca acgttctgcg
     4921 gttgatggga gtggcggcgt gatcgtcgac atcaacgcgt tcatcggcaa atggccttac
     4981 tggcccatcg cggcaacgtc gcccgcggaa gtgtcggcac aactggcggg ctggcggatc
     5041 gaccgcgcgg ccgtctgctc cacccgaagc gtcttcgtgg actggcagga cggcaactgc
     5101 gaagtggaac aggctagcca cgccgaaagc cggttcatcc cgttcgcgtg cctggggaca
     5161 ctcgagctgt cgcacgccct gccgcgcacc ggctacgatt ttgaaggcta tgccgcgcgc
     5221 ggcttccgcg gcatcaggtt gtatccgcaa catcatagtt atcatccgct cttcaattca
     5281 ttcaccggca atattctgga gcaggccgaa atgcgccagt ggccggtcct gctgccgctg
     5341 cgtatcatca tgaattgggg aatgcccatg ctcgaactgg gcgtgatcga agcgttggtc
     5401 gagaggcatc cccgggtcat ctggattctc gccggaatca actacctcca cgagctcgaa
     5461 ctggcggtca cactgctgcg caggaacgcg aacgttcacc tggagacctc ttgcgtcatg
     5521 ggctatcacg cgatcgccaa gctggtggag cagtgcggca gcgagcagat tctgttcggc
     5581 agcggcgctc cggttcagca cggtgcgggc gtagccaaaa tcgcgaaggc aaaaatctcc
     5641 gaggccgacc gcgaacggct gctggaaaag aacgcgctcc gtcttctgag gctcgaagag
     5701 aaatgagaat cctgcgcttt ttttcagacg acggaagcgt gcacctgggc gtgcgcgacg
     5761 gcgaatgggt aacggacctg ggagcgagca gccccatctc gctgatggac ggaaagcctc
     5821 ccaccagcgg ccctcgtgtc ccctacgcgg gcctgcgcct ggatgcgccg atacgcgatt
     5881 gcccgaagct gctggcgttg gcgggcaact atcgggaaca cgtgcgggag gcggggatag
     5941 aagacctcgc accttcggcg aacatcactc cacaggtttt ttggaaaccg tctacgacgg
     6001 ggatcaaccg gcctggcgga gtgattccca tccggccgaa caatgtgttc gtcgattggg
     6061 aaattgaact ggcggtggtg atcggccggt ggacgaagaa cgtaaaagca gaggccgcga
     6121 tggagtccgt gttcggatac acgattctga acgacatcag cgagcgcaaa ttcaattcgc
     6181 tcctggaagg caggcacgta cgccagttcg accccttctt tgactggctg atgggtaaat
     6241 ggttcgacgg acatgcaccg ctcggcccgg agatcgtgac ggcagacgaa gtgccggacc
     6301 cgcacaacct cggcatccgc ctttcggtca acggcgagct catgcaggat tccaacacgc
     6361 ggcatatgat cttccgcatt ccggaaacca tcgcttatat cgcatcggtt ctcacactgg
     6421 agccgggcga tattatcgcg acgggaacgc cgcatggcgt tggttcggcc cgcggcatct
     6481 gcctgaaacc gggcgacgtc atacggggcg agatcgaagg attggggatt ctcgaaaacg
     6541 ttattgcatt ggaggagagt tgaaggacaa ggttcgaatc ggcatcatcg gctccgggtt
     6601 catgggccgc accaacgcgg agaccgtgac gcgctatctg gaacgggcgg aactcgtgtc
     6661 tgtagcgggt ggcagccgcg cgccggcact tgcggccgaa tatggagtga caagcgccgc
     6721 atccgtggat gccctgctcg cccgcgacga tatcgatgcc gtgctgatca gcaccccaca
     6781 cgccgaacat tgcagccagg ccgtggccgc ggcccaacgc ggaaagcata ttctgctcga
     6841 caaacccatg gcggtgacag tggaggaatg cgaccggatt ctcgaagccg ttcgtgccgc
     6901 gcgagtgaag ctgatgatca tgttcggcca gcggttccgg atctgcaata tcgaggcgcg
     6961 ccggctcata agagaagggg ccatcggcaa ggtaaagatg atcctggagc tgattctggc
     7021 gagcggcggc cttcagacgc tgccgccgtg gcagtcgcta gcggaaaact acgggccgtt
     7081 cctgggacac gcggtgcata atctcgatcg catccgctgg ctcaccggcg cggaatttac
     7141 cagcgtgagc gcgcaggtgc agcgcgatgc cgcaacgggg aacgaagtct ccacgatggc
     7201 gctctacgga ctgagtggag gcgcggcggc gacggtatgg gaatcgtggg acctggcgcc
     7261 tccggcgttc ccgcgctccg cctcgggcgc gtggatcgtg ggtgaaacgg gcaacctgga
     7321 tctggacgcc tacggccaac tgcggctcgg aaaaaacaaa gagtggaccg tcgtggccga
     7381 gcaacctccc atcgactggc aggggcaagg tatgctctcg ccggtccgca tgcaggccta
     7441 ccgggaccag catcaggagt tcatcaacag tattctcgaa gatcgcgaac cgtcggtaac
     7501 cggggcggac ggccgtgcgg cggtggaagc cgcacaggcc gcataccggt ccgaggcgga
     7561 aggtattacg attcatcttc gagcgaagga gaccgcgcaa tgatcgaaca gcttgccgtg
     7621 gacggaggtc ccagagcagt ggaagaaaag ctgcccacct ttctggagag cgccggccgg
     7681 agctttggag tggaagagga gagcctggta ttggaggcct tgcggaacgg ttgtctcagc
     7741 cgtaacggag gcaccatggt cttgcaattc gaacaggagt tcgcgcaagc gctcggtgtt
     7801 ccgcatgccg tggcctgttc gagcggaacg gcatcgatac acctcgccgt cgcggccctc
     7861 aacctcgaac cgggggatga ggtcattgtg ccgccgatta cggatatcgg cacggtgctg
     7921 cccgtgttgt ggcagaacgc cgtgccggtt ttcgccgatg tggatccgcg gaccatgtgt
     7981 ctcgatccgg cggatgtagc gggcaggatc tccggccgga cgcgcgcggt gatcgcggtt
     8041 cacctggcgg gacagccttg cgatatggat gcgctggctc gcatttgcag ggagcgttct
     8101 ctcgtattga ttgaggactg ttcgcagtcc tattgggcgg agcacgaagg ccggctggcc
     8161 ggaaccatcg gcgacatggc gtgcttcagc ctgcaacaga gcaagcatat gacgtgtggc
     8221 gaaggcggca tcctggtgac gccgcgcgag gactacgcgc gccgcgcgcg cctgttcacc
     8281 gataaggcgt ggcccagaga gacgaagtcg ctcggctcct gccgcttcct gttcctggca
     8341 caaaactacc gcatgagtga gttggcggga gcggttgcgc tcgcgcaact ggcgaaagta
     8401 cgcgacacgg tggcgcggcg cagagcgtcg gcggaacggc tgacggcgtt gcttgcggag
     8461 gtcccaggcg taacgcctcc ccatgttcct tcgggtacgc tgcactcgta ctggttatac
     8521 atgctgcgtg tgaacgaagc cgaagcgggc gtcacagcga gacgattcgg ggaagcgctg
     8581 acggccgagg gcgttcccgc ctgggtggaa tacatcatcg atccactcta tttgtcaccc
     8641 atttttacgg aatcgcagac ctacggcacc tccggttatc cactgcgcga gttcggccgt
     8701 caaaattacc aacgcgggtt atgcccgaat gccgaggcgg tgctcaagag catcattgcg
     8761 atctggtgga acgaaaacta cacggaggcg cacgtccacc agatctcgga tgcgatccgc
     8821 aaggtcggga gccatttctc tctcgcgggt gcgcggcaat aggtgcggga ccatgccgaa
     8881 atcggatatt tactacatcg aagtactcgg caaggcgctg gatattctcg atgtgttcgt
     8941 cgaagtgcgg aagccgagac tgacgctgca agagatctcg acccttacca agctgaacaa
     9001 gaacaccgtt tttcgagcgc tctatacgct ggccgagcac ggctacatca tcaagcgcca
     9061 ccacgaatat gaacttggtt ccaagctgct cgatctgagc aacgcgaaac tgcaacggcg
     9121 cgacctggtg gcggccgccg ggccttacat ggacagcctc cgcgagaagt ttggggaaac
     9181 ggtcaacctc ggcgtactcg atggcggggc gatccggtat gtggatgtgc gggaaagccc
     9241 gcaccgcttc cggctggcgg aacgggtcgg cggcagcgat tttctgcact gcaccgcgct
     9301 ggggaaatgc cacctggcgt ttatgccaat ggaagaggtg cgccggttgc tgaaagaaca
     9361 gggaatgccg cgcgaaaccc cgcgcaccat cgtaacgctc accgcgatga aggccgaact
     9421 cgaacgcgtg cgccggcagg gttatgccgc cgaccccgag gaaagcatgg aaggcgcttt
     9481 ttgcctggga gcaccgatcc tggacgatac cggcgctccc atcgcggctt tgagcatttc
     9541 ggggccgacg acacgcttca acgcctccgt gacaccggag gcgagcaaag cgctgctgga
     9601 cgccgtttca gagattcgag caaggctcgg atacacccgc cggcaggaag tcttacagga
     9661 acaaatacgc aataaggctg caacgggatc ttgataatta gtcaaacgca gttatactgt
     9721 ctataaccat ggtgtctatt gaggaactgg ctaaagaatc ggggcctctc cgtttcaccg
     9781 gtttcgacct gtaccgcata gccattccga tgcgtgaacc gttccggatc agctcgggcg
     9841 aagtggcagt caaagattcg ctccttctga aaatcaacgg tggagacgca ttcgggtggg
     9901 gtgaatcgtc cgccatgccc ggtgcatttt attccagcga gaccccggat tcgtgctggg
     9961 aggatctctc aacagcggtt ctacccgctc tcgccagccg gtggtttccg gatatggtct
    10021 cgctcgaaga gttcctgaaa gagcgatcgc agagcgcctt cgttcgggta gcagttgaga
    10081 ccgccgcatg ggagctgttg gcgcgccgga agggtatttc tttgcggtcg ctcttttcga
    10141 ttgcggatcg cgacgtggtg tccgggctcg ccgtggggct ctatgacacg cgcgaagaac
    10201 tgcgggaagc cctcgaacgc taccgtccac gcgactacgc ccgtctcaag atcaagatca
    10261 agcggggaca ggatgtcgag ctggttcgcg cggtccgcga atggttcccc gatgttcggc
    10321 tcttcgtcga cgccaacgcg gattacggca gagaagattt tgccacgttt caggaattgg
    10381 atgccttcgg cctgatgatg ttcgagcagc cgctggcaag gagtgaattc gaagcctccg
    10441 cggagcttca gcggcttgtg cgcacgcccg tgtgtctcga cgaaagcatc gaaaccgctg
    10501 acgacgcgcg gcgcgcgatc cagttgggaa gctgccgcat cgtcaacatc aagctccagc
    10561 gggtgggagg ctacctcgaa gcgcttcgga ttatcgagat ctgttcacgc agcggcattc
    10621 cggtatggat gggcacgatg cccgaactgg ggatcggcag cgcacaggcg ttgcagcttg
    10681 ccgcgcatcc cgcgttccgc ttccctaccg atgtagagcc aagtgaacga tggtacgagg
    10741 acgacattct tacgcctcgc ctgtctctcg ccaacacccg gcttcccatc ccggccggac
    10801 ccggcatcgg gtttcaggtg gacgagcgaa agctggagca atactgtacg gcccgaaaaa
    10861 ccttcaagac ataagccatg gagcacatgc accttgcggc ggcggattac ctggtagtcg
    10921 gcgggtactt cgtcattgtt ctgtggatcg ggttctattt ccggaaccgc ctggaagccg
    10981 cgaaggatta tttcgccggt ggaaaccaga tcccatggtg gctggcgggc atttcgcact
    11041 acatgtccag cttcagcgcc tttgccttca tcgcttacgc acagatcgga tattcgcacg
    11101 gctggatcgc ggtctcgctg ttctgggcca ccatcccggg ctgcctcatc ggtggactgt
    11161 acttcgccca gcggtggcgg cgggcgcgga tcatcacgcc aatccagttt ctcgaagccc
    11221 gttttaacgg atttgtccgg cagttgttcg catggtccgg cattccgatg aagatttttg
    11281 acgacgctct aaaagtcttc gcgaccggcc tgtttgtttc tgtcgcggcg gggttgaatc
    11341 ttcattggtc catcgcggtg tgcggccttg tcatggtggc ctacacgttt ctcggcgggt
    11401 tgtgggcact ggtggtgacg gactatgtcc agtttctgat gaaggcgctg gccattctgc
    11461 ttctgttgcc gctcgcggtg gtgaaggccg gcggatggca gcgctccttc agcgctttgc
    11521 cggacgggtt cctgcaacct atgggcgggc cgttcaattg gacctatctg gccgggttca
    11581 ccattctgat gatgatcagc tacaacgcaa gctggtctct ggcgcagaag tattactccg
    11641 tgcggaacga gaaagaggcg gcgaaggtcg cctactgcgc gggagcgctg aacctgatcg
    11701 gcgcaccgct gatgattctt ccggcgctca tcggccggaa ctttctcccc aatctcgtgg
    11761 agcagaaccg caccgccgac gtctacgttc tgctggtaat ggagctgctg ccggtaggaa
    11821 tgatcggcat catcatggcg gcgatgttct cggccactat ggcgatggtc agtgcggact
    11881 tcaatgcgat cgccagcgtg ctgaccaagg acgtctatca ccgcttgatc cgtccggagg
    11941 cgtcggaaaa gcatctgctg aacgcaggcc gctggatcac cctggccctg ggactgctga
    12001 ccacattgct tgcgctgtgg gtcgcagcgt tccggcaaca gtccctgttc aacaccatgg
    12061 tgacggtgct ggggttgttc atggcgccga cgttcctccc ccttctggcc gggctcgcca
    12121 ttcgcaagct gacctggcag ggcgcatttg ccggatttct gtgtgggctc gtcaccgggt
    12181 tcgccatgct ggcggtaaaa acatggtggc tccctccggc ttcggacgcc gggagttcgt
    12241 atttctttga aggaatctcc ctgctggcga atacggcggc gacgatcctc ggcatgttcg
    12301 cgggcaccgt gctttcgcgg tcgagcgccg ggcgggagcg caagactcaa cagttcttcc
    12361 gtgagttgga ccggccgatt gcgccggagg aagttcccgc gaggagcagc aatcccgcag
    12421 cgtccgtgtt gggactgtct acgatcggcg tgggcgcttt gctgttcctg gccggagcgg
    12481 tctccggttc ggcaaccgcg cgggtcatag attttgcggt cggcgccgtg ctgatcgtta
    12541 tcggtgtctt ctttcgccgc agggccggac aatcttccgg aaaacccgcg gctgtggtta
    12601 cgacaagttg aagggaacta ctttaaatga gcacttcaaa gaaagccctg accgcgttgc
    12661 tgatgttttc agcggccact tttacgtatg cgcaaagcac agatgccacc attctaggcg
    12721 tggtacgcga cccttcgggc gccgtcatcg gcggcgtcga agtgatcgcc acgaacgtcc
    12781 ggaccaatgt ggcaaaaaga gtcttctcga acgatagcgg caattacgag atccccgcgc
    12841 tacagccgag tgagtacagg ctggaagcaa catcgaaggg attcaagaag caggtcctta
    12901 cggggattat cctccaggtg aaccaggcgg cgcggctcga catcgttatg caattgggcg
    12961 atgtggtgga ggccgttacc gtagaggcca ccgcattgct gattcaaacc gataccgggg
    13021 cggtaggcca ggtcatcgac aaccgcaaga tcgtggactt gccgctgaac gggcggaact
    13081 tcacacagtt ggctaccctg actcccggcg tgacgccgtc gggcgcgatg gggacgggac
    13141 gcgcacaatc ggtatttgtc agcggctccc gcgcaaccaa gacggaattt gttttggacg
    13201 gcgtttcctc caccggtccc atcaacggag gaaccggcat tctgccgtcg attgacgcgc
    13261 tggaagaatt caaggtgcag accagtgcgt tcgccgccga attcggccgc tctccggcgc
    13321 tggtgaatat atcgatcaaa tcgggttcca acgcatttca cggcagcgcc ttcgaatttg
    13381 tgcgcaacga cgttttcgac gcgcgcaaca cctttgcgct ttcgaatcct cccctgaagc
    13441 gaaaccagtt cggagtgacg ttcggcggtc cgctaaaaca gaatgacttc tttttctttg
    13501 gcaactatga agggctccgg caacgccgtg gggcgacgtt caacgcggtg gtgccgacgg
    13561 cggcgcagaa aaacggagat ttcggatcca tcgcgatctt cgacccagcc accacgaggc
    13621 tggaaggatc cacctatgtg cgcagcccct tccccggcaa tcgcataccg gcggaccgca
    13681 ttgcgcccga ggccgcttac ttcctgaagt tctttcccga agggaacaat tccgatggag
    13741 cgcgatatat cgtttcaccg agcaacagcg acgacaacga tcagttcacc atccggctgg
    13801 ataaaaaagt gaaaagcgcc ggacttctct tcggccgcta caccttctcg aacgtaaaga
    13861 ccttcagccc gtcggcattt ccggcgctgg ttggcgtaat gctccggtcc cggttccaga
    13921 acgccggggt aacttacacg cacacctggc ggcctacact gatctcggag acgcggctcg
    13981 gatacaatcg cgagtacacg ctggaggatg cgccgggcat cggtacgaat cacaccgtgc
    14041 tctcgggaat caccgggttt gaccgtacca gcgagagtgt tccgcgtttt ccgcagatcg
    14101 gcatcagcgg attcaccggg atcgacggcc gaaccttccg gccgctgaac aataccaacg
    14161 atatctacca gattatccag acagtgtcct ggtacacggg caagcacacg ctgaaggtgg
    14221 gagccgacct gcgccagcag aggaatacga actggaacac ggcctacaat agcggcaatt
    14281 tcagcttcag cggcacgtac acgcttaatc cgggcgtggc tcgatcgggg tccgggttcg
    14341 cggacttcct gctcgggctg ccggcatcgg caaaccggtc cttccctcgc gatgctttcg
    14401 gcaatcgctt cggcaactat catttcttcg ctcaggacga ctggcgcgcg acacagaacc
    14461 tgaccatcaa cctcggcctg cgatacgaat tcaacccgtg gccgctcgga taccgcaatc
    14521 agttgacgct gttcgaccgc agcctgggca aagtgatcat ctccgatccg atcaatttcg
    14581 aggcgcagaa aatcaccagg gccgcttacg atgcgatgcc ggatgcttat gtcacaacca
    14641 aagagttggg gttgccccag cagattcagt cgaacgacct gaacaatttc gctccccgcg
    14701 tcggattcgc gtggcgcgtg ctcggcgaca accgcacggt gctgcgcggc ggttacggcg
    14761 tgttctacga actggtgaac ggcaacggcc gtaccggagg cgtcatcaat cctccgttcc
    14821 tgttcgacga agtggcaaat aacaacaacc ctattccgaa ccggacgctg cacgactttt
    14881 tcttcacgca gccgccttcg gctgcatcgc ctcccatcat cgactcgcgt ccgctcaatc
    14941 aacgcacacc ttacgaacag acgtggaacc tcatgatcca gcgcgaactg tttgcgaaca
    15001 tggcgctcga agtcggctac ctgggcaaga agggaactca tctcgaaaga gacgtgttgt
    15061 tcaatcaacc ggaatttcca ggacccggca gtatccagtc gaggcgtccc ttcccgcgct
    15121 tcggctccgg cacgctgcgc gatgacggca gcaacagcac ctatcatggc ctgcaaacga
    15181 agctggagag gcggttctcg aatggcctct cgttccttgc agcctacacg tgggcgaaat
    15241 ccatggacga cgtcagcacc gatctcggcg gggcggtcca gaactggaag gattaccgcg
    15301 cggagcacgc ggtttccgat ttcgacgtcg ctcacaactt cgtcttcagc gggttgtatg
    15361 agctgccgtt tggtccggga aagccggtgg cctcgggcgc cggtggtgct ttgagccggc
    15421 ttatcggcgg ctggcagctt ggcgcgattg tgcagcagcg cagcggtctt ccgtttacgc
    15481 cgcgcatcag cctcgaccag gcgaacaccg gaacgtcgca gcggccgaat cgccttggta
    15541 ccgggaccgt cgatcaacgg actactgcaa gatggttcaa cccggccgat ttcacgattc
    15601 cggcacaata cacgttcgga aactccgggc gcaatatcct gcgcgccgac cgctataaca
    15661 atatcgatct ggtgctgctc aagaacacca cgattaccga gcgggtttcg acacaactgc
    15721 gcttcgagtt cttcaacgca ttcaaccatg ccaactacgg cgccccgaat gcagtggtca
    15781 ataccgccac cgccggacag gtggcttcgg caaacgatcc cagaattctc cagttcggat
    15841 tgaaggtagt gttctgagtg gcgcatctcc ggaggctggc ggagatatga tcgggtacgg
    15901 aggatgatga acttgctcaa agggttttac cgggcaatac tcgggaccgg tctgctggcc
    15961 gccgccgttt ccgccgcgga gttacgcgtc gcggcgttcc gcgccgacgc cacaccggcc
    16021 atgggggaac cgctgatttg gatacagccc acaacgaaag tggaagaccc gctgttggcg
    16081 aaaggtgtgg tgttggacga cggaaagcac cggtacgttc tatgctccgt ggactggtgc
    16141 ggactgggcg gctccacgca tctgctgttc cgccgcaagg cggctgccgc ggcgggcacc
    16201 gacatctccc gcgtcaccat tcattccgtt caccagcaca cggcacctta catcgatggc
    16261 ggcgcatatt cgctgatgcg gacgtttccg aaccctccgt tattgatgag cgatgaatat
    16321 ctcgaagccg tcacagaccg tttggccggc gcggtgaagg aggccgtgaa gaggcttcag
    16381 cctttcgata gcatcggcgt cggagaggcg cgggtggagc gggtggcttc ggcccggcgc
    16441 attcacgacg acggcaagct cgtcacccgc tacagcacgg gagccaagga cccgaagatg
    16501 gctgcgcttc cggaaggcga gattgacccc gccgtaagga cggtcactct ggccaacggc
    16561 ggcaaggctc tcgtgcgtct ccactactat gccacgcatc cacaaacgtt ctgctgcgat
    16621 ggccgcgtgt ccggcgattt cgtcagcacg gcgcgcgaga agcttgaaca ggatgaaggc
    16681 atcccacagg tctactttac gggatgcgcg ggtaacgtga cagtcggcaa gtacaacgat
    16741 ggatccgaaa aggcgagggc ggaactcgcc cggcaacttc gaaaggggtt cgacagctcc
    16801 atcgacgcga ctcgattcca taaagcagcc gcgctacggt ggaagaccac cgatctgaag
    16861 ttgcctccca aaccgggggt ccccaccgat ccgtccgccc tgaagggcaa gagcggcgtg
    16921 gatctctacc gctcggcgca ggcgaccgca ttctgcatgc gcgccgagcc gctcacgatg
    16981 agcttgcttg aaatcggccc ggccaggatc atacatatgc ccggcgaacc catgctcgaa
    17041 ttccagaaat atgcccttgg ggtgaaccgc agtccgttcc tggcggttgc gggctatggc
    17101 gacatcagcc cgggctatct ttgcacggac caggctttcc gcgaaggcgg ttatgaacca
    17161 agcgcttcca atgcggggcc cggtaccgag gcacagatta agaaggcgat tgataagtta
    17221 ctggaaaaat agttgataag gagatacgat gtctagctct cacaaaaatt cggggccggg
    17281 tgagacccgg cgctcattcg tacaaacagg gggagccgcg ctcgccgcca tgggagcggg
    17341 caccgcggct ttcggcaaaa ccgaaacact ggctctcaac ggcggggcga agtcggtcag
    17401 ctttcccacg gcgaaagaat ccgcgctgac caaatggccc cgttacggcg caaccgaaaa
    17461 gaaagtgctg catgagctta tcgacagcag caagttctac gaggaactgc cgctcttcga
    17521 gaacgagtgg aaggcttata cgaagtcccc gttcgtgaaa gcgcacatga acggatcaag
    17581 cgcgctcacc agcatgtatt ttgctctcga ccttcc
//
LOCUS       DASTMK010000566         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_285385, whole genome shotgun sequence.
ACCESSION   DASTMK010000566 DASTMK010000000
VERSION     DASTMK010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285385"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFQ79_23300"
     CDS             complement(<1..301)
                     /locus_tag="VFQ79_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEU0142669.1"
                     /translation="MYPLKKGKIATQAHVGLPQGTYEEEHGRRGFYGKSAHLYHAHPP
                     TGWIRFEGKLRPHAFDCNQLEPSDRADAQGEGIAFLGNDDVTLYVSRRSEPMPFYY"
     gene            388..1296
                     /locus_tag="VFQ79_23305"
     CDS             388..1296
                     /locus_tag="VFQ79_23305"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEU0142670.1"
                     /translation="MPAVGLTTTEAPFIEQAKTEGKLYIQQPYELYSRENHEAWRKLY
                     HRIVPRWQKYANEHFLRGIDSLRLDPGAVPRLDNVNRFLKPLTGFQAKAVSGYVPAFL
                     FFDCLRNREFPTTITIRRSDKLDYLPEPDIFHDVAGHVPMHTDKAFAETLVRFGESAH
                     TAAELVSDIRDERKRIRTLTSIIKAMARFFWFTVEFGLMRGAGGLRVYGSGLLSSYGE
                     IEHAVESPEVQRYPIQLEWAVNQSFEIDHYQPLLFIVDSFDHLFDLVGRLEQWMKSGK
                     LNNVAPGEPAVNEQDLESFLEADWRT"
     gene            1335..2357
                     /locus_tag="VFQ79_23310"
     CDS             1335..2357
                     /locus_tag="VFQ79_23310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0142671.1"
                     /translation="MRLAIAALLVVSLAAQESEFGVESRLVLVPVTVTDTKGRSVDGL
                     EASDFLLYDNGVRQKAVVDTIATGVAPVALIVAVQSSGISAPVLAKVQKIGAMLQPLI
                     TGERGCAALVAFDERTRWLQECTGDPDLLVRAFDNLRPGEYKSAHMLDAVHKAVEHLL
                     KRPNFRRVLLLISESRDRGSERTLEAVLITAQAAGVTVYAATYSATKTAFTTRTSEKD
                     RPPESKIPRDSRTGPITPQGRVITPTPEQQLDILGGIGELVRLGKVKDTEVLTSKTGG
                     TAFPFTRQKGLEGAIEKLSAELHTQYVLSFTPEAPASGYHRLEVRINRGDQFRIRARP
                     GYWSSE"
     gene            complement(2445..2666)
                     /locus_tag="VFQ79_23315"
     CDS             complement(2445..2666)
                     /locus_tag="VFQ79_23315"
                     /inference="COORDINATES: protein motif:HMM:NF023276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HEU0142672.1"
                     /translation="MELRADVIVIDDGGARRLARLLGLQVVGTLGVLLQAKYLGLVPA
                     LRPLLDALRPLPFHMKKSLYDALLNAAGE"
     gene            complement(2864..3139)
                     /locus_tag="VFQ79_23320"
     CDS             complement(2864..3139)
                     /locus_tag="VFQ79_23320"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HEU0142673.1"
                     /translation="MTTNIPVGGDLVGLLETLNRPLEDAVRELIVLEFYRQRRISSGK
                     AAELLGMSRIGFIEYSSRLGVPFFDVTPEELQAEVALLRQHRSSEKP"
BASE COUNT          703 a          954 c          885 g          636 t
ORIGIN      
        1 gatagtagaa cggcatcggc tcggaccggc gcgacacata caacgtcaca tcgtcattgc
       61 ccagaaaagc gatgccctcc ccttgcgcgt ccgcgcggtc cgatggttcc agttggttgc
      121 aatcgaaggc gtgcggcctc agtttcccct caaagcggat ccagccggtt ggagggtgtg
      181 catgatacag gtgcgcgctc tttccgtaaa agcctctgcg cccgtgctct tcttcatacg
      241 tgccctgggg taatccgacg tgtgcctgtg tggcaatctt gcccttctta agcgggtaca
      301 tccagcatcc ctccctgcct tcagcataga tcacaccaca taaaagtggg gcataatcaa
      361 agtagagagg gggcggcttt gggaaccatg ccagccgtgg gcctgaccac caccgaggcg
      421 ccattcatcg agcaggcgaa aaccgagggc aagctttaca tccaacagcc ctacgaactc
      481 tattcccggg agaaccacga agcctggaga aaactctacc accgcatcgt tccccgctgg
      541 cagaaatacg caaacgagca cttcctgcgc ggtatcgatt cgctccgtct cgaccctggc
      601 gccgttccca ggctcgataa cgtcaaccgg ttcctgaagc cgctgaccgg tttccaggcc
      661 aaggcggtca gtggatatgt tcccgctttc ctgtttttcg actgcctccg caaccgcgag
      721 ttccccacta ccatcaccat ccgaagatcg gacaagctcg actatcttcc cgagcccgac
      781 atcttccacg acgttgccgg gcatgtgccc atgcatacag acaaagcttt cgctgaaacg
      841 ctggtgcggt tcggagaatc tgcccatacc gccgccgaac tcgtctcaga catcagagat
      901 gagcgcaagc gcattcggac actgaccagc atcatcaagg ccatggcccg cttcttctgg
      961 ttcaccgtag aattcggatt gatgcggggc gccggtggcc tgcgggtgta tggcagcggc
     1021 ctgctgagtt catatggcga aatcgaacac gccgtcgaat cgcccgaagt gcagagatat
     1081 cccatacaac tcgagtgggc cgtgaaccag tcgttcgaaa tcgaccacta ccagccgctg
     1141 ctgttcatcg tggattcctt cgaccacctc ttcgatctgg tgggccgcct ggagcaatgg
     1201 atgaaatccg gcaaattgaa caatgttgct ccgggcgaac ctgcggtaaa cgagcaggat
     1261 ctcgagagct tcctggaggc ggactggcga acgtaacggc tacctcggcc actggattgc
     1321 acatcagaac ggtgatgagg ctcgcgatag cggcattgct ggtagtctca ctggccgccc
     1381 aggagtctga attcggtgtc gaatcccgcc tcgtactggt tcccgtaacg gtgacggaca
     1441 ccaaaggccg ctccgtggac ggccttgaag catcggactt cctcctctat gacaacggcg
     1501 tccgccagaa ggctgttgtg gatacgattg ctacaggtgt tgcgccggtc gcactgatcg
     1561 tcgccgtcca gtcctccggt atttccgctc cggtgttggc aaaagtgcag aagatcgggg
     1621 cgatgctcca gcccttgatt accggagagc gcggctgcgc cgccctggtt gcgttcgacg
     1681 aacgcacacg gtggctccag gaatgtactg gcgatcctga cctgctggtg cgtgccttcg
     1741 ataaccttcg ccccggagaa tacaagagcg cacacatgct ggatgccgtt cacaaggccg
     1801 ttgaacacct gctgaagcgt cccaatttcc ggcgcgtttt gctgctcatc tccgagtcgc
     1861 gcgaccgagg cagcgagcgc acattggaag ccgtgctgat aacggcgcag gccgccggtg
     1921 tgacggttta tgcggcaacc tactcggcaa ccaaaacagc attcacgaca agaacatcgg
     1981 aaaaggaccg tccgcccgaa tcgaagattc cccgcgatag tcgcactggg ccgatcacgc
     2041 cacaagggcg agtgatcacg ccaactccgg agcagcagtt ggacattctg ggtggaatcg
     2101 gcgaattggt gcgccttggg aaagtcaagg ataccgaggt cctgaccagc aagaccggag
     2161 gtaccgcgtt tccgttcacc aggcaaaaag gtctggaggg cgccatcgaa aaactcagcg
     2221 ccgaattgca tacgcaatac gtgctcagtt ttacaccgga agctccggcc tccggctacc
     2281 accgcctgga agtccgcatc aaccgcggcg accagttccg cattcgcgcc cgccccggat
     2341 attggtcgtc agagtaggcg gagcaggggc cagcatgttg agatcggccg caatgagctt
     2401 gccgaaggag acctctgaag tgtcaccggc tttcaacgtt cgtttcattc cccggcagca
     2461 ttgagcaggg catcgtataa gctcttcttc atgtgaaatg ggagcggccg gagagcatcg
     2521 agaagcggac gaagtgctgg aaccagtcct aaatatttcg cctgtaggag gactcccaaa
     2581 gttccgacaa cctgaagtcc gagaaggcgt gcaagccgcc gtgcacctcc gtcgtcaatt
     2641 acgattacat ctgctcgtag ttccacggcg agagcgatag cctcgctttc acctgatcca
     2701 agagaagcgc tcagaatctg cgcagcaagt ggctgctcca gttggcgcac atggatccag
     2761 gcaggcaagg ttcgtcgacc cagaacctca gacgctaccg caggaggaat cagaatcggg
     2821 ctcaagcgca atgatcggac tggtattgga aacgattacc aactcagggt ttctcagaac
     2881 ttcgatgttg tctcagaagg gcgacttcag cctgaagctc ctccggcgtg acgtcgaaga
     2941 acggaacgcc tagtcgcgac gaatactcaa tgaatccgat tcggctcatt ccgagcagtt
     3001 cggccgcttt accactcgag atcctccgct ggcgataaaa ctccagcacg atcaactcgc
     3061 gcacggcgtc ttcgaggggg cggttcagag tctcaagcaa tccgaccaga tctccgccaa
     3121 cgggaatatt cgttgtcata aacagcgtat gcttgccgct aagtattctt tatgctcg
//
LOCUS       DASTMK010000567         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_156339, whole genome shotgun sequence.
ACCESSION   DASTMK010000567 DASTMK010000000
VERSION     DASTMK010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156339"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1852
                     /locus_tag="VFQ79_23325"
     CDS             224..1852
                     /locus_tag="VFQ79_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0142674.1"
                     /translation="MLSVLALSLVPSAGVAQSDVLIYGCTSGAITAAVQVVRMGKSVQ
                     IVCPERHLGGLTAGGLGFTDTGNKAVIGGLSREFYHRVWQHYDKPEAWRWQKREEYGN
                     KGQGTEALDKTARTMWIFEPHVAGNVYEAWIKEAKIPVVRNAWLDREKGVKKQDGRIV
                     SITTLDGNTYSARMFIDATYEGDLMAAAGVDYHVGRESTSTYGEKWNGVQTGVLHHRH
                     HFGAVKTPISPYVIPGDPKSGVLPRISTEPPGNYGEGDKKVQAYCFRMCLTQAPANRV
                     PFPKPANYDPKEYELLLRIFAAGWRETFQKFDAIPNAKTDTNNHGPFSTDNIGRNWDY
                     PEASYERRREIIQEHVNYQQGWFYFIANDPRVPADVRTEMAKWGLAKDEFKDNGNWPH
                     QLYVREARRMIGKYIMTENELLKRRPTPEPVGMGSYGIDSHNIQRYITPEGYVQNEGD
                     IGVSTNGPYQISYGSLVPKKGQTENLLVPVCVSSSHIAYGSIRMEPVFMILGQSAATA
                     AVMAIDMDLPVQDVPYEKLRERLLADGQILESKR"
     gene            complement(1859..>2227)
                     /locus_tag="VFQ79_23330"
     CDS             complement(1859..>2227)
                     /locus_tag="VFQ79_23330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142675.1"
                     /translation="ASFEQIPWYWFIPAAGIAALAWYWGQKGRNVRAFWAVCVWSVAG
                     IFALKAIALEPLDHRASARGLWRQIAGQRQDVCVSGLHRAWRYGLNYYSVEPLPDCHE
                     EPKPIEIRQKDNEIPAISPR"
BASE COUNT          499 a          684 c          652 g          392 t
ORIGIN      
        1 catcggtgaa aaagggacgg tcaagatgcc gctgcaggag acattctggg ctgcccggtt
       61 cgggatgctc acggatcaat ttggaatcaa ttggatgttc gacctggaaa agccggaaca
      121 aaaataaccg gacgctcttt aagaatcgca cccccggcgg gcgcgattct gccttatcgc
      181 ataaagtagt gggtagctca tgactttcac gcttccccgc tttatgcttt cggtactcgc
      241 gctctcgctt gtaccttcag ccggcgtggc ccagtccgac gttctcattt acgggtgcac
      301 gtccggcgca atcaccgccg cggtacaggt tgtgcgcatg ggcaaatccg tccagatagt
      361 ttgtccggag cggcacctgg gcgggctcac cgccggcggc ctgggattta cggacaccgg
      421 caacaaggcc gtgatcggcg ggctttcccg cgagttctac caccgcgtct ggcaacacta
      481 cgacaagccc gaagcctggc gctggcagaa gcgggaagaa tacggcaaca aaggccaggg
      541 aacggaggcg ctcgacaaaa ccgcccgtac gatgtggatt ttcgagccgc acgtcgccgg
      601 gaacgtgtat gaggcgtgga tcaaagaagc gaaaatcccg gtggtccgca acgcatggct
      661 cgaccgcgag aaaggcgtta agaagcaaga tggccgcatc gtctccatca cgacgctcga
      721 tgggaacacc tattcggccc gcatgttcat cgacgctacc tacgagggcg atttaatggc
      781 ggcggccggt gtcgactatc atgtcgggcg cgaatccacc agcacgtacg gcgaaaagtg
      841 gaacggagtg cagaccggcg tgcttcacca tcgccaccat ttcggtgccg tcaagacgcc
      901 gatctcccct tacgtgattc ccggcgaccc caagagcgga gtgcttccgc gcatcagcac
      961 cgagccgccc ggcaactatg gcgaaggcga caaaaaagtg caggcctatt gtttccgcat
     1021 gtgcctcacg caggcgcccg caaaccgcgt gccgttcccc aaacccgcca attacgatcc
     1081 gaaggaatac gaactgctcc tgcgcatctt cgccgccggc tggcgcgaaa cgttccagaa
     1141 gtttgacgcc atcccgaacg ccaagaccga cacgaacaac cacgggcctt tcagcaccga
     1201 taacatcggg cggaattggg actacccgga agccagctac gagcgccgcc gcgaaatcat
     1261 ccaggagcat gtgaactatc agcagggctg gttctatttc attgcaaacg acccgcgcgt
     1321 gcccgccgat gtacgcacgg agatggcgaa atggggcctg gcgaaggatg aattcaaaga
     1381 caacggaaac tggccacacc agttgtacgt gcgggaggcc cggcgcatga tcggcaagta
     1441 catcatgacg gaaaacgagc tgctaaaaag acggcccacg ccggaaccgg tcggtatggg
     1501 ttcctacggc atcgactcgc acaacatcca gcgctatatc acgccggagg gctacgtcca
     1561 gaacgaaggg gacatcggcg tcagcacgaa cgggccttac cagatctctt acggttccct
     1621 ggtgccaaag aaaggccaga cggagaatct gcttgtgccg gtgtgtgtgt cgagctcgca
     1681 tatcgcgtat ggctccattc gtatggagcc cgtcttcatg atcctcgggc aatccgccgc
     1741 cactgctgcc gtgatggcga tagacatgga cctcccggtg caggacgtcc catacgaaaa
     1801 gctgcgggag cggctgctgg ccgacgggca gatcctcgaa tccaagcggt agcgggcttc
     1861 atcgcgggct gatcgccgga atctcgttat ctttttgccg gatctcgatc ggcttcggct
     1921 cctcgtggca gtccggcagc ggctccaccg aatagtagtt cagcccgtag cgccaggcac
     1981 gatggagacc ggaaacgcag acgtcctgcc gttgtccggc gatctgccgc cataaaccac
     2041 gcgcggaggc tcggtggtcc agcggctcga gggctatcgc tttcaaagcg aaaattcccg
     2101 ctacgctcca cacgcacacc gcccaaaacg cgcgcacatt cctgcctttt tggccccaat
     2161 accacgccag agcagcaatg ccggccgccg ggataaacca gtaccaggga atctgttcga
     2221 aagaggc
//
LOCUS       DASTMK010000568         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_188822, whole genome shotgun sequence.
ACCESSION   DASTMK010000568 DASTMK010000000
VERSION     DASTMK010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188822"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFQ79_23335"
     CDS             <1..569
                     /locus_tag="VFQ79_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142676.1"
                     /translation="LFGNAVKTPELKSVNVTIDLLPERRVASIENAWVASSEVAAGAE
                     VPVKVFLRPYRGERLEREFDVKIPAGLPKGDYKVVLSDAGTLNRMQTAAGTMNRFIDL
                     PQTVSLINQERSNNKLYVSLVQARPTLYVEDKTMPSVPASVLNVMQAGRTGGSRSYLT
                     SAETAQEQMSIPFDYVINGSYSLKLTVK"
     gene            575..2779
                     /locus_tag="VFQ79_23340"
     CDS             575..2779
                     /locus_tag="VFQ79_23340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142677.1"
                     /translation="MKNSVFPIPALLALAVGVYAVETQFWEQSDQPDYEKATLNGVSV
                     RSDGRLSLAPVFQEVFEASTPYIWSLAEDSKGNVYAGGGGSDGSTAKLFVVDAKGAGK
                     VLTALDGLQIHAIAVDRNNRVYAGTSPDGKIYRISSSGDAQVFYDPRAKYIWGLAFDS
                     HGNLFAATGDAGEIHKVTPNGEGSVFFRTEETHARSLAIDSKDNLIAGTEPSGLIFRI
                     SPKAEGFVLYQGDKREITSIALGRDGFIYAAGVGTKQAPAIVPQVPPVLAPPPTAPPK
                     STSQIVPAQRPGAAPPLTLSAPVAVSGGSEVYRIDTEGYPRTIWSDTQEIAYALAFDD
                     AGRLLIGTGNKGNLYRVDSEVLSTLLVNAPPTQITALVSGRGGRVYAATGNIGKVYRV
                     GPELQASGSVESDVFDSGGFSYWGRLWFDGQRNGGKLSFATRSGNLDRPQQNWSPWAS
                     VSAEETGGRIASPPARFVQWRLELALSPDNRSPEVSTVEVAYLPKNVAPVVEKIEATP
                     TNYRYPQPPVMAAENRNLTLPPLSRRRRPASSASITPSEPSSTLNYAKGYVGARWLAS
                     DENGDELTHKVEIRGVRESEWKLLKENVSQRHISWDSTAYADGEYVLRVTASDLPDNS
                     PAGALTGSLVSPAFLIDNTPPAITGLTASRTGKDSSVSWTARDANSIIERAEYSLDGG
                     DWLVAAPESRLSDSPELSYKLTLENLPPGEHTIAVRVTDAFGNQTVGKAIVR"
     gene            2884..3966
                     /locus_tag="VFQ79_23345"
     CDS             2884..3966
                     /locus_tag="VFQ79_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0142678.1"
                     /translation="MSSAMAADPTYRVVSSYKRIPGAGMPGSYPGQVVAVHSPKSVDD
                     NTEKIDADAVKEMMTRGMLALTGAPDLRQAWGRFIGKDDVVGIKVNCVGRPNVMSSPE
                     VVAEIVRNVNAVGVPLERIHIYERFGDQMEECKYPEKVPAGVHYFGAEQTRGTNEPYD
                     PLMYVDVNFFGEEDTRSNIIRLVSREITKIINVPNIKDHGASGVTGCLKNIAYGSFSN
                     VARSHSGSVTHTRTFIGALAASEPVRSRTVLQVVDGIKGVWHGGPFSTDKRFRFLPKQ
                     MMFGTDPVAIDRLILDIVDNQRKAMGAISVWDRDPRHLRNKDTPAGPDPNVNAYIREP
                     GHIEYASTLGLGVYELEKIRVKRIDI"
BASE COUNT          839 a         1121 c         1264 g          822 t
ORIGIN      
        1 ggcttttcgg aaacgccgtg aagactcccg agctgaagag cgtgaatgtc acgatcgacc
       61 ttttgccgga acggcgcgtg gcgagcatcg agaacgcgtg ggtagcctcc agcgaagtgg
      121 ccgccggtgc cgaagtgccc gtcaaagtgt ttctccgccc ctatcggggc gaacgcctcg
      181 aaagagagtt tgatgtcaaa atccccgcgg gcctgcctaa aggcgattac aaagtggtgt
      241 tgagcgacgc gggtaccctg aaccgcatgc agactgcggc gggcacgatg aaccggttca
      301 tcgatcttcc gcaaaccgtt tcactcatca accaggagcg aagcaataac aagctttacg
      361 tttcgctggt gcaggcgcgt cccactctgt acgtggagga caagacgatg ccgagtgtgc
      421 ccgcttccgt gctgaatgtc atgcaagccg ggcgcacggg cggcagccgc tcgtatctca
      481 cttccgccga gacggctcag gagcagatga gcattccgtt cgactacgtc atcaacggca
      541 gctattcgct caaactcact gtcaagtaga acacatgaaa aattccgttt tcccgattcc
      601 ggcgctgctg gcgcttgcgg tgggtgtgta tgccgttgag acacagttct gggaacagtc
      661 ggaccagccc gactatgaaa aagcgacact gaatggcgtt tccgttcgca gcgacggccg
      721 tttgtcgctg gcgcctgtat ttcaggaagt gtttgaagct tcgacaccct atatctggtc
      781 tctcgccgaa gattccaaag ggaacgttta cgcgggcgga ggaggttccg acggctctac
      841 cgccaagctc ttcgtagtgg acgcgaaggg cgccggcaag gtgctcactg ccttggacgg
      901 gttgcagatt catgcgatcg ccgtggatag gaacaaccgc gtttatgccg gtacgtcgcc
      961 ggatggaaag atctaccgca tctcatcgag tggcgatgca caggtatttt acgatccgcg
     1021 cgccaagtac atatgggggc ttgcgttcga cagccatggt aatctttttg ccgctaccgg
     1081 cgatgcgggg gagattcaca aggttactcc aaatggagaa ggttctgttt ttttcaggac
     1141 tgaggagacc cacgccaggt cgctggcgat cgactcgaaa gataatctca ttgccggaac
     1201 tgagcccagc gggttgatct tccggatttc gccgaaggcc gaaggctttg tcctctatca
     1261 gggggataag cgcgagatca cctccattgc cttgggacgg gatgggttca tctacgccgc
     1321 gggagtcggt acgaaacagg caccggcaat cgtgccccag gttccgccgg tacttgctcc
     1381 acctcccacg gcgcctccca agagcacctc gcaaatagtg ccggcgcagc ggccgggcgc
     1441 cgcgcctccg ctgacgctgt cggctcccgt agcggtatcg ggcggatccg aagtttatcg
     1501 tatcgacacc gaaggatatc cgcgcaccat ctggagcgac acccaggaaa tcgcttatgc
     1561 gctcgctttc gatgacgcgg gacggttgct gatcggaacc gggaacaagg gaaacctgta
     1621 ccgcgtagat tcggaagtac tctcgacgtt gttggtgaac gcgcccccta cgcagattac
     1681 cgcgctggtc tcgggacgag gcggcagggt gtacgctgcg acggggaaca tcggaaaggt
     1741 gtacagggtt ggcccggagt tacaggcgtc gggctcggtg gaaagcgatg tgtttgattc
     1801 cggcggcttc tcgtattggg gccggttatg gtttgatggc cagcgaaacg gcggcaagct
     1861 gagctttgcg acccgcagcg gcaatctgga ccgtccacag cagaactgga gtccgtgggc
     1921 ttccgtttct gcggaagaga ccggtgggcg tatcgcttct ccgcccgcgc ggtttgtgca
     1981 gtggagactc gaactggcgc tgtcgccgga caaccgctcg ccggaggtga gcaccgttga
     2041 agtggcgtat ttgcctaaga acgttgcacc cgtggtggag aagatcgagg ccactcccac
     2101 caattaccgc tatccgcagc cgccggtgat ggcggctgaa aatcgcaacc tgacgctgcc
     2161 tcccctttcg cgccgcagac gtccggcatc gtccgcgtcg attaccccca gcgagccctc
     2221 ctcgacgctg aattatgcaa agggctatgt aggcgcccgg tggcttgcat cggatgagaa
     2281 tggtgacgaa ctcacgcaca aagtggaaat tcggggagtg cgggagtctg agtggaagct
     2341 gctgaaagag aatgtatccc agcggcacat cagttgggat tccacggctt acgccgacgg
     2401 tgagtatgtc ctgcgagtga ccgcctccga ccttccggac aactcgcccg ccggcgcgct
     2461 gaccggaagc cttgtcagcc cggcgttcct gatcgacaac acgcctccgg cgattaccgg
     2521 tttaacggca tcccgtacgg gtaaagacag ctcggtgtcg tggacggcca gagacgcgaa
     2581 cagcatcatc gagcgtgcgg agtattcgct ggatggaggc gactggctcg tggcggcacc
     2641 ggagtcccgg ctgtcggact cccccgaatt gagttacaag cttacgctcg aaaacctgcc
     2701 ccccggtgag cacaccatcg cggtgcgggt taccgatgcc tttggcaacc agacagtagg
     2761 taaggccatc gtccggtaat atagacaggt attgcgactt cccagagagg tgttgctttg
     2821 agtgtattcg ataccttcga ccggcgaaca tttctgcgta catgtacggc tggagcggca
     2881 gtgatgtcgt cggcgatggc cgcggaccct acgtatcgcg tggtgagttc ctacaagcgg
     2941 attccgggag ccgggatgcc tggctcttat ccaggacagg ttgttgcggt gcattccccc
     3001 aagtcggtgg atgacaacac cgagaaaatc gacgccgatg ctgtcaagga gatgatgaca
     3061 cggggcatgc tggcactgac tggggcaccc gatctccggc aggcatgggg acgctttatc
     3121 gggaaggacg acgttgtagg cattaaagtg aactgtgtcg gcaggcccaa cgtgatgtct
     3181 tctccggaag tggttgccga gatcgtcagg aatgtgaacg cggttggcgt gccgttggaa
     3241 aggattcaca tctatgaacg attcggcgac cagatggagg agtgcaagta ccccgaaaaa
     3301 gtgcccgccg gcgtgcatta cttcggcgct gaacagacac gcgggacgaa tgagccctac
     3361 gatccgctga tgtatgttga cgtgaacttc ttcggggagg aagacacccg ttcgaacatc
     3421 atacggctgg tctcgcggga gatcacgaaa atcatcaacg tgccgaatat caaggatcac
     3481 ggagcttccg gtgtcacagg ctgtttgaaa aacatagcgt atggatcttt ttcgaacgtg
     3541 gcccggtctc attccggctc agtgacgcat actcgtacgt tcatcggagc cctggccgcg
     3601 tccgagcctg tgcgttcgcg tacggtgctg caagtggtgg acggcatcaa gggcgtttgg
     3661 cacggcggac ccttctcgac ggataagcgg ttccgttttc tgccgaagca gatgatgttc
     3721 ggcactgacc cggtcgccat agaccggctg atactggata ttgtcgataa ccagcgaaag
     3781 gcgatgggtg cgatctcggt gtgggaccgc gatccacggc atcttcgcaa caaagacacg
     3841 ccggccggtc cggatccgaa tgtgaatgcc tacattcgcg aaccgggcca catcgagtat
     3901 gcatccacac tcgggttggg agtctacgag cttgaaaaga tccgcgtgaa acggatcgac
     3961 atttagccgc ttatggggct gcgcgcccgg cggcctgcgc ctccggaatc aggtggtccg
     4021 atggatcgat agcggcgaat gtgata
//
LOCUS       DASTMK010000569         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_476274, whole genome shotgun sequence.
ACCESSION   DASTMK010000569 DASTMK010000000
VERSION     DASTMK010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476274"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFQ79_23350"
     CDS             complement(<1..478)
                     /locus_tag="VFQ79_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU0142679.1"
                     /translation="MDFRQMKVNEHDFGIMCYDPSYSNTAACKSKITYIDGDAGILLY
                     RGYPIEQLAEECSFLECAYLILYGELPTETELNGFRGEVMAETMIHENLKKFMDGFLY
                     DAHPMSMLASTMAALSTFYPDSVDIKDAANRRKQIMRIIGHVPTLAAYCYRRRRGLP"
     gene            complement(958..1356)
                     /locus_tag="VFQ79_23355"
     CDS             complement(958..1356)
                     /locus_tag="VFQ79_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0142680.1"
                     /translation="MTPGEILRRYRKIAVVGISNNRTRASNGVSRYMLGQGYEIVPVN
                     PAITEFEGIAAVPDLASAPADIEIVNIFRRPEYVPEIVEAAIAKGAKVIWMQQGIVHE
                     EAAERARKAGLEVVQDRCILVEHMAQKHEG"
     gene            complement(1365..3389)
                     /locus_tag="VFQ79_23360"
     CDS             complement(1365..3389)
                     /locus_tag="VFQ79_23360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0142681.1"
                     /translation="MATRMQLIGRYEIERELGRGAMGVVYLARDTRIGRSVALKTIRL
                     SDFADAEKLQRLRERLVREAQSAGILSHENIVTVYDVDEQNGLSYISMEYVEGDSLER
                     MLENRRELSRDTLLSLLRQMAEALDFAHSRGIVHRDIKPPNIMVTPKGRVRITDFGIA
                     KIAHASSVTQGGGMLGTPDYMSPEQIAGTAVDGRADQFSLGVIAFELLTGEKPFVAET
                     LPALFYRIAHQPSPDAVLLNRSLSPEIGKVLERAMAKDAVDRFPTCLAFVDALTEACA
                     ATPGWFALPRGMSQTLPTMGGAASPNQPTAEEPRSIPTPAPPPVPPSPAARTEEPVWV
                     PTLPPLRADSSPRASYDKPVGPEGGAGGGGFITGADRSQAETLEMPGPSPQAWLRDVE
                     EEERREKQSRRRGIWMALLSFLVVAGALAAVYFGLGGISIPGITQRSGQFGTNPGVGE
                     RPSSTEGETATGPEHPQATSPTEPPAASQAEETPEAEHPDVEASAPEITTPEAGHPAA
                     TPVPEPVKPQAPARTEPAKPIGPERPATRKATVRFMSDPPGASVTVDAEAEYRCDTPC
                     SLDIPLGRHTYSMRLPGYRNEIKLIDLDEAGETVSVRLERRVGMVRVTSNPAGATIYV
                     NGERQQGVTPFTLRLLPGNYRVSIEKEGRRVERNVRVTEDSAVQIDAVLR"
BASE COUNT          676 a         1082 c         1029 g          768 t
ORIGIN      
        1 agggcaggcc gcggcggcgc cgataacagt atgcggccag cgtcggaacg tggccgatga
       61 tccgcatgat ttgcttgcgc cggtttgcgg catccttgat atccaccgag tcagggtaaa
      121 aggtagagag cgcggccatc gtgcttgcca gcatgctcat tgggtgggca tcgtaaagaa
      181 agccatccat gaacttcttg aggttttcat ggatcatcgt ctcggccatc acctcgccgc
      241 gaaagccgtt caactcagtt tccgtaggca attcgccgta gaggatcagg tacgcgcact
      301 ccagaaatga acactcttcg gccagttgtt cgatgggata tccccgataa agcaggatgc
      361 cggcgtcgcc gtcgatatag gtgattttgc tcttgcaggc ggcggtattg ctgtacgagg
      421 ggtcgtagca cataatgcca aaatcatgct cgttcacctt catctggcgg aaatccatcg
      481 catgaacggt ctcgttttct atcgggattt cgtagttctt cccagttcgg ttatctgtaa
      541 tgctgagtgt gtcttttccc atgactcgcc tgcctgaaga aatctagagt gtatggaacg
      601 ggctgatgga ccggtcctgg ccgaaccacg tgccgggtgt ttccatcccg acagaaaggt
      661 tatacctatc agatactccc acggaagaaa tggtgtcgta tccgtaggga ggaatcggcg
      721 gatctcccag tctgctgcga caccaatctg aggaacggaa tacgggctgc gggttcccga
      781 acttccgatt gccaatccgc tattctatca gccggaacca agcgatgcct tgagaaatgc
      841 gattcccgcg cacggttgcc tgcctggatg gtacggaata gtccagagtg tgtccgtttc
      901 ttcccaatcg gttccactct gccctggcgg acagtcccta gggcgatgcc gccggcccta
      961 gccctcatgc ttctgcgcca tatgctccac caaaatacag cggtcctgta caacttcgag
     1021 gcccgccttt cgtgcccgct ccgcagcttc ttcatgcacg atgccttgtt gcatccagat
     1081 caccttcgcg cccttggcga tcgccgcttc tacgatttct ggcacatact ccgggcggcg
     1141 gaaaatattg acgatctcga tatccgcagg cgccgaggca aggtccggca cagcggcaat
     1201 cccctcgaac tccgtgatcg cggggttcac cggcacaatt tcgtaaccct gccccaacat
     1261 gtaacgggaa acgccgttac tcgcccgcgt gcggttgttt gaaatgccta cgacggcgat
     1321 cttcctgtag cggcgaagaa tctctcctgg ggtcatccga actcctaacg aagtactgcg
     1381 tcaatctgca ccgcggaatc ttcggtcacc cgtacattcc tttcgacgcg gcgaccttct
     1441 ttttcgatac tcacccggta atttccaggg agcaaccgaa gggtgaatgg agtcactccc
     1501 tgctggcgtt cgccgttgac gtagatcgtc gcgcccgccg gattcgaggt caccctcacc
     1561 atcccgacgc gtcgctccaa tcgtacgcta acggtttcac ccgcttcgtc gagatcgatt
     1621 agcttgattt cgtttcgata tccaggcaac cgcatggagt acgtatgccg gcccaacggt
     1681 atatcgagcg aacaaggtgt gtcgcaacgg tattcggcct ccgcatccac agtcacagag
     1741 gcgccgggcg ggtcgctcat gaagcgaacg gtggctttcc tggtggccgg ccgctccggc
     1801 cctatgggct ttgccggctc ggttcgcgcg ggcgcctgtg gttttaccgg ctccggaacc
     1861 ggggtggcgg cgggatggcc ggcttccggg gttgtgatct caggcgccga agcttcgacg
     1921 tccggatgct ccgcttccgg cgtctcctca gcttgggatg ccgcaggggg ttccgttggg
     1981 gatgtcgctt ggggatgctc tgggccggtt gccgtttctc cttcggtgga ggaaggtcgc
     2041 tctcccacgc ccggattcgt accgaattgt cctgaccgct gtgtaatccc cggtatggag
     2101 atgccgccca ggccaaagta aacggcagcc agtgcgccgg ccaccaccag aaagctcaac
     2161 agcgccatcc agataccgcg gcggcggctc tgcttctcgc ggcgttcttc ctcttccacg
     2221 tcgcgaagcc acgcttgcgg tgaaggcccc ggcatctcca gggtttccgc ttgggaccgg
     2281 tctgctcctg ttataaaccc gcccccaccc gctcccccct cggggcctac gggcttgtca
     2341 tacgaagcac gggggctcga atccgcccga agcggtggca gcgtgggaac ccagaccggc
     2401 tcttccgttc gtgcagccgg ggagggcggc acgggagggg gcgctggcgt tggtatggaa
     2461 cgtggttcct ccgcggttgg ttgatttgga cttgcagcac cccccatcgt gggcagcgtc
     2521 tggctcattc cacgtggaag ggcgaaccag cctggagtgg ccgcgcaggc ttcggtgagt
     2581 gcatccacga acgccaggca ggtggggaag cggtctaccg catccttcgc catggcccgt
     2641 tccagcactt ttccaatctc agggcttaag gaacgattca agagcacggc atcgggagag
     2701 ggctggtgag cgatccgata aaacagcgct ggaagcgttt cggcaacgaa gggcttctcg
     2761 ccagtgagca gttcaaacgc gatgacgcca agggaaaact gatccgcgcg gccatcgacg
     2821 gcggtcccgg cgatctgctc cggcgacata taatccggcg ttccaagcat tccgccgcct
     2881 tgcgtcacgc tggaggcatg ggcgatcttg gcgatcccga aatccgtgat ccgcacccgc
     2941 cctttcggcg tcaccatgat gtttggtggt ttgatgtcgc ggtgaacgat gccccggcta
     3001 tgcgcaaagt cgagcgcttc cgccatttgc cgcagcaggg aaagcagcgt atcgcgcgaa
     3061 agttcccggc gattctcgag cattcgttcg aggctgtcgc cctccacgta ttccatggag
     3121 atataggaaa gcccgttctg ttcatccacg tcgtagacgg taacgatatt ctcgtgcgag
     3181 agaatgccag ccgactgggc ttcccgcaca agccgctccc gcaggcgttg gagcttctcc
     3241 gcgtctgcaa aatcgctcag acgtatcgtc ttcagcgcca cggagcgccc aatgcgcgta
     3301 tcacgggcca gatagactac gcccatcgcg ccccgcccaa gttcgcgctc tatttcgtaa
     3361 cgcccgatca actgcatacg cgttgccatt gtagcgcaac gattgcgcca aatgcctgaa
     3421 aaacaagggc gcaaaacgtg cccgaaatgg ctcattgcta caatggtggc gaatgattca
     3481 aacgcttttt gggtccgttc cgcaggaacc caccttcttt gagaaacttc agcagggcat
     3541 ccggaaaact cgcga
//
LOCUS       DASTMK010000570         9190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_605349, whole genome shotgun sequence.
ACCESSION   DASTMK010000570 DASTMK010000000
VERSION     DASTMK010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605349"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..404)
                     /locus_tag="VFQ79_23365"
     CDS             complement(60..404)
                     /locus_tag="VFQ79_23365"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0142682.1"
                     /translation="MKKHRVVRGIRVEEGSGNVYADLGYADSDSMLVKAQLAAKIAEI
                     VQRRALTQTRAAEILGLTQPKVSALLKGRFRGISEHRLLECLTRLGRDVHIVIKPAPR
                     SRSNGRLTLSVA"
     gene            complement(401..703)
                     /locus_tag="VFQ79_23370"
     CDS             complement(401..703)
                     /locus_tag="VFQ79_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0142683.1"
                     /translation="MPSPVMDLFGYALYLAQAGKKHEQAKPLRGFGSAGVLEVVEDWD
                     RSTYRAVYTVQFKSAVFVLHVFQKKSKRGVATPKADIDLICERLTVAERIAKELSR"
     gene            complement(821..1990)
                     /locus_tag="VFQ79_23375"
     CDS             complement(821..1990)
                     /locus_tag="VFQ79_23375"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0142684.1"
                     /translation="MDSSRVAAALKRFQLTEAEIHAWVEVSPREPFHEGPLAGVPFGV
                     KDIFETRGLRTGYGSEIYRDRMGTRDAAIVTRLCEQGAVLFGKTETTAFAYFDPAATR
                     NPRNPAHTPGGSSSGSAAAVAAGVVPFALGSQMMGSIIRPASFCGIAGFKPTHGVLPV
                     DGMLPFAPSLDTVGFLAESTAVCRQVWHALGFPIAAPPRVRFAVPEGLPAVLPDMQLA
                     FDYAAELLANNGHSLECVRLPVPYEDLLSAVRQVNEYEGARSHYGLWKEFGTRIGEKL
                     AGLVARGIAICEEEYRRQLLLLRDAASKADAMLGDDMVLLTPAAPGPAPAGLESTGDP
                     IMNAVWTGLGTPALSIPIPHGSGLPLGLQLIARRGAEAALLYAGALVEEVFGKRG"
     gene            2054..2986
                     /locus_tag="VFQ79_23380"
     CDS             2054..2986
                     /locus_tag="VFQ79_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142685.1"
                     /translation="MKRSLITTIVFLFAIILCASSAAPADRSRLRFIAAGAGEFTFDT
                     GLLSGKLRGEGKSVGLLPVVHIPSGATLTRSMGFAGHYRVFSTNNRYGPGAWYWPSET
                     KLAGDGSVETHWSADAERPFDMWAVYRWAAPGTLDVETRVLPSTDIPDFEVFEAQYFS
                     EGFSESLVYARGGPGGKPAFVPAEQSEGVWQMFPRDGEAVKMIQDGRWKIPPSPVDWV
                     IRPALEQPVGIRRHGASGLAIVMMAPPEDCFAVSTPHQAEGHYSMYFSLFGRTLKAGE
                     PARARVRIMLMTAPTDNEVLDAYTAYLRELRGRR"
     gene            complement(2991..3935)
                     /locus_tag="VFQ79_23385"
     CDS             complement(2991..3935)
                     /locus_tag="VFQ79_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142686.1"
                     /translation="MMKSALVLFVLFFAAVAGAAELRLGLVGTDTSHVIAFARMLNDA
                     SAPGHVPGARIVAAYKGGMPDNATSAKYIDQYTEELRTKWGVEIVPDIPALCSKVDGV
                     LLTSVDGRPHLKEARQIFAARKPVWIDKPLASTLDDAREIARLAKEAGVPWWTSSSLR
                     FSEMITALKGPGITGAMTWGPGPFEEHHHLDLSWYAIHPIEMLYTLMGPGCVEVTRIH
                     TEGADEIVGRWKDGRVGSVRALRPYGSYGAVVFKGKETLQSDPKAAIGYRPMVVEIVK
                     FFETGVVPVPPAESLEIFAFMDAALRSKQSGGKPMPLR"
     gene            complement(3932..4957)
                     /locus_tag="VFQ79_23390"
     CDS             complement(3932..4957)
                     /locus_tag="VFQ79_23390"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142687.1"
                     /translation="MKQFGAAVLGTGDVSGEHIKAYQQNPHTEVRAILSRDKAKAEAK
                     AREYGLVNCRAYTDVEELLKSSDIHMVSICTPHHLHVEQGVACAQAGKHVVVEKPIAL
                     DLDGLRKLETAVRENNVRSVVSFVLRWNPLYETIKTILADGTIGELYHAEVDYLHGIP
                     KSYSGYEWISKREYGGTALLTGGCHAVDGLRWFINKEAVEVFGYANFSKSNPIGYEYE
                     PNSVTLVRFADGTTGKVGCSIEYMAPYTFSIVLMGDGGVMRDNRLFTKHWPGQTGWAA
                     IPTILPDSGAVTHHPFIGEIDHFVDCILTGKESHCNVADAVKTHEICIGSELSRREGK
                     PVRLPLS"
     gene            complement(4954..5838)
                     /locus_tag="VFQ79_23395"
     CDS             complement(4954..5838)
                     /locus_tag="VFQ79_23395"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEU0142688.1"
                     /translation="MTTRREILCAAGFALAGGPLRLSDTKGEKLAVSYGGKTLLEYRY
                     SSGRPKPYVHPLCLAGGEPLTLDAPEDHIHHRGLMVAWSEVNGIDFWGETNPARHGQI
                     VHQRFERKQVGAAAEIVSINHWVAEGKLLLIEKRTLKVPPPWEQGVWVDWTTELKAPS
                     EPVKLAAGQHVYNGLGIRFVHSMDGGGVLNSNGTNTIEKANGDAAQWCAYYGKLDATG
                     TAGVAMFDHPQNPRHPNAFFVMNKAFGYMSAAPTFRAPFDLAVNRPIRFRWGVLAFSG
                     EPKPDLLNRRFQSWKVEG"
     gene            5954..6988
                     /locus_tag="VFQ79_23400"
     CDS             5954..6988
                     /locus_tag="VFQ79_23400"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0142689.1"
                     /translation="MNGYQRIAAVLGGKPPDRVPVMLHNFLMAAREAGVCMREYRRSP
                     RAIARCFIESVERYGYDGVVVDVDTATLAGAVGVPVDYPDDAPARCSGEGKIGSLEEV
                     ADLTPPNLQKDAGIETWLEATAILKRHFGDEVYIRGNCDQAPYSLAASMRSPEEWMMD
                     LMDEANHERAHKLLAWCTGAVSEFIRLMAATGAHMVSNGDSWGSPDLVSPRLYREFAL
                     PHEKSVVSAAHVAGKPYFLHICGKTDRILPDMVSTGADGLEIDYKTDVRLAHDTLRST
                     TVFIGNLDPSGVLANGTAALVEEKTRELLRVFSDTPRFILNAGCAIPADTPPDNIKAM
                     IQTAREHRPM"
     gene            7004..8350
                     /locus_tag="VFQ79_23405"
     CDS             7004..8350
                     /locus_tag="VFQ79_23405"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142690.1"
                     /translation="MKTTSALNALSRRDVLQAAVSAALAAPAVVPASALGLGGQTPPS
                     DRISLGIIGVNGMGRSNLANCAKHPDVVVTGICDVSTDRREKALELHRATAKGYNDYR
                     ELLARKDIDAVIIASSPHWHALMAIDAVKAGKDIYLQKPMTLYPDETLAVRNAVNRHK
                     RISQIGTQIHAGSNYRRVVEWVRSGKLGPISVVRTFNVMNQGVNGIGHAPKEDAPKGL
                     DWGMWLGPAAERPFNALLYADSYNHCSFWDYSFGWTPGMAPHIIDLPIWALNLGVPEV
                     TSCTGGRYVIRDDGDAPDTQEATWRYPNFTMTWMMNCANSFAFDFGRGKPARRLGIYF
                     HGVNATLFTDYGKHEIVPEGDFLKDSTPPPESIAASPGHEREWLDSVKSRKEPSCSVN
                     YHYKVDLALTLANLSYRLGRSVRFDPKMEKIVGDKEAVKLARPTYRHPWKFPAEYL"
     gene            complement(8372..>9190)
                     /locus_tag="VFQ79_23410"
     CDS             complement(8372..>9190)
                     /locus_tag="VFQ79_23410"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEU0142691.1"
                     /translation="WGEGAGAVDLTEPTRQALVDSYVESFSKTPLVMLLTDEKTNKYG
                     ISKKPVGWRVDCLGDMGGFNNPNWSHMQDYYPQAIINFGMEDAWKKAPVSLEVCWVMQ
                     HWKNKGWDVDYIIDQSLKWHISSFNGKSSAVPDEWWPQVNRWLKKMGYRFVLRKFTYP
                     AAVSSQGKIAFTTWWENKGVAPIYKEFPLALRLKGTQKTEVLLTDADIRTWLPGDNLY
                     DNAVFVPAGMPGGEYELAIGILDPQTRVPAVKLAIAGADTEGWYPLGKIQIAGN"
BASE COUNT         1840 a         2869 c         2601 g         1880 t
ORIGIN      
        1 gcaggaggtc gcaggttcga gccccgcacc gcccaccata aaaacgtcca ccgcttccct
       61 tatgccacag acagcgtcag tcgtccgttc gaacgactcc ttggcgccgg cttgataaca
      121 atgtgaacat cccggcccag ccgcgtcagg cattccaaaa gtcggtgctc ggaaatcccg
      181 cgaaaccgcc ctttcaaaag ggcggaaacc ttgggctgtg ttaaccccag gatctctgct
      241 gcacgggttt gcgtaagcgc ccgccgctgg acgatctcgg cgatcttggc ggccagttgg
      301 gctttgacca gcatgctgtc gctgtccgcg tagccgaggt cggcatacac gttgccggag
      361 ccttcctcga cacggatgcc ccgtacgact cgatgttttt tcatcgtgat aactccttcg
      421 cgattcgctc cgcgaccgtg aggcgctcgc agattaaatc gatatcggct ttgggcgtgg
      481 ccactccacg cttggacttc ttctggaaca cgtgcagcac gaacaccgcg ctcttgaact
      541 gcacggtgta gacggctcga tacgtgctcc gatcccaatc ttcaacgacc tccaggacac
      601 cggcagagcc gaagccccta agaggcttag cctgctcgtg ctttttcccc gcctgggcaa
      661 ggtaaagggc gtatccaaag aggtccatga ctggactagg caacgactgt agatcccgtt
      721 tcgaactccc cacccagtgc agtggtttca gggatggaga tttatccatc tctgttcatt
      781 atacccaaat tggtataagc gccggggtgt gaatgtgatt ctaacccctt ttcccaaata
      841 cctcctccac cagcgcaccg gcatagagca gggcagcttc cgcgccgcgg cgcgcaatca
      901 gttgtaggcc caagggcagc ccgctgccgt ggggtatggg aatcgataac gctggcgtgc
      961 ccaggccggt ccacaccgcg ttcattatcg gatcgcctgt cgattcgagt ccggcgggtg
     1021 caggtccggg tgcggcggga gtgagcaaca ccatatcgtc tccaagcatg gcgtctgcct
     1081 tcgaggccgc atcccgcaac aggagcaact gccggcgata ctcctcttcg caaattgcga
     1141 tcccgcgagc caccagtccg gccagcttct ccccaatgcg tgttccgaat tctttccaca
     1201 gcccatagtg tgaccgggcg ccttcatatt cgttcacctg gcggactgcc gataacaggt
     1261 cctcataagg aacgggaagc cgcacgcatt ccagagagtg cccgttgttc gcgagcaatt
     1321 ccgccgcata gtcgaacgca agctgcatat ccggcagcac agccggcaaa ccttcgggaa
     1381 cggcgaaacg aacacgggga ggcgcggcaa tcggaaaccc caaggcatgc cagacctgcc
     1441 tacagaccgc cgtgctctcc gccagaaacc ccaccgtatc gagcgaaggc gcaaacggca
     1501 gcatgccatc caccggcagc acgccatgtg taggtttgaa gccggcgatc ccgcagaacg
     1561 acgccggacg aataatggag cccatcatct gcgagcccag tgcaaacggg accacacccg
     1621 ccgcaaccgc cgccgccgat ccgctcgaac ttccgcccgg cgtgtgggcg gggtttcgcg
     1681 gatttcttgt ggctgccggg tcgaagtaag cgaatgccgt ggtctcggtc ttcccgaaaa
     1741 gcacagcgcc ctgctcgcac aaccgggtga cgatggcggc gtctcgtgtg cccatgcggt
     1801 cccggtagat ttccgacccg tacccggtgc gcaggccgcg agtttcgaaa atgtccttta
     1861 cgccgaacgg aacgccagcg agcgggcctt cgtggaaagg ctctcgcggg gacacctcca
     1921 cccaggcatg aatctctgct tcggtgagtt gaaagcgctt cagcgccgcg gcaacacggc
     1981 tggaatccat caggcgcctg ggcctcgccg cgctggggtg cggaggtata ctggtcggcg
     2041 gttcagccaa gtcatgaaac gctctctgat aaccaccatc gtttttcttt tcgccattat
     2101 cctatgcgcc tcctctgccg cgcccgcgga tcgctcacgt ctgcggttca tcgccgccgg
     2161 cgccggtgaa ttcaccttcg ataccggttt actctccgga aaattgcgag gagaaggtaa
     2221 atccgtcggc ctgttgccgg tggttcacat cccttcgggc gctactctta cccggagcat
     2281 gggattcgcg ggacattatc gcgttttttc caccaacaat cgatatggac cgggcgcgtg
     2341 gtattggccc agtgaaacga aactcgccgg ggacggttcc gtagagacgc actggtccgc
     2401 ggacgccgaa cggcctttcg atatgtgggc ggtctacagg tgggcggcgc caggaaccct
     2461 cgacgttgaa acgcgcgtgc tcccgtccac ggacattccg gatttcgagg tgttcgaggc
     2521 ccaatacttc tcggaaggat tttcggaatc gctggtgtat gcgcgaggcg gtccgggcgg
     2581 caagcccgcg ttcgttcccg cggagcaatc cgagggcgta tggcaaatgt tcccgcgcga
     2641 cggtgaggct gtaaagatga tccaggacgg acggtggaag atcccgccaa gccccgtcga
     2701 ttgggtgatc cggccggcgc ttgaacaacc tgtcggtatt cgccgccacg gtgcctcagg
     2761 attggcgatc gtcatgatgg cgcctccgga ggactgtttt gccgtgtcga cgccccacca
     2821 ggcagaaggc cactactcga tgtacttttc gcttttcggc cggacgctga aggccggaga
     2881 acctgcacgc gcgcgcgtcc ggattatgct catgacagct cccacggaca acgaagttct
     2941 tgacgcctac acggcgtacc ttcgcgaatt gcgcggccgc cgttagagct tcagcgcaac
     3001 ggcatgggtt taccgccgga ttgcttactg cgcagcgccg cgtccatgaa cgcgaaaatc
     3061 tccaggctct ccgccggcgg caccggaaca acgcccgttt cgaagaactt cacgatctcc
     3121 acaaccatcg ggcgatagcc gatggcggct ttcggatcgc tctgtagtgt ttctttgcct
     3181 ttgaacacca cggctccgta agagccgtat ggacgtaatg cccgcacgct ccccacgcgg
     3241 ccgtctttcc agcggcccac aatctcgtcc gcgccctcgg tatggattcg cgtcacctcc
     3301 acacaaccgg gccccatcaa ggtgtagagc atctcaatag gatggatggc ataccaggag
     3361 agatcgagat ggtggtgctc ctcgaacggg ccgggtcccc acgtcatcgc tccggtaatc
     3421 cccggtccct tcaacgccgt gatcatttcg gaaaaacgca ggctggaact ggtccaccac
     3481 ggcactccgg cttctttcgc cagccgcgca atttcacgtg cgtcgtcgag cgtggaggcc
     3541 agcggcttat cgatccaaac aggcttccgg gcggcaaaaa tctggcgcgc ttctttgagg
     3601 tgcggccgtc cgtccacgct cgtcagcaga accccatcca ctttggagca aagcgccggg
     3661 atgtccggta caatctcgac gccccatttc gtgcgcaact cctccgtgta ttgatcgatg
     3721 tatttcgcgc tggtggcatt gtccggcatt ccgcctttat aggcagccac gatacgcgcg
     3781 ccgggaacat gaccgggcgc cgaagcatcg ttcagcattc tcgcgaaggc gatgacatgc
     3841 gacgtatccg ttcccaccag gcccagacgg agttccgccg cgcccgccac ggcggcgaaa
     3901 aacaggacga acagaaccag cgcacttttc atcatgacag tgggagccgg accggcttcc
     3961 cctcccgccg agacagctcg cttccaatac aaatctcgtg tgtcttcaca gcatcggcca
     4021 cattgcagtg cgattccttg cccgtaagaa tacagtccac aaaatgatcg atctcgccga
     4081 tgaacggatg gtgcgtgacg gcgccactgt caggaagaat ggtgggaata gccgcccaac
     4141 cggtctggcc gggccagtgt ttcgtgaaca ggcgattgtc gcgcatgacc ccgccatcgc
     4201 ccatcaatac gatgctgaac gtgtacggcg ccatgtattc aatggagcaa cccaccttgc
     4261 ccgtggtccc atcggcgaac ctcaccagcg tgacgctgtt aggctcgtac tcataaccga
     4321 tcgggttgct cttgctgaag ttcgcgtagc cgaagacttc caccgcttct ttgtttatga
     4381 accagcggag gccgtccacg gcatgacagc cgccggtgag cagcgccgtg ccgccgtact
     4441 cgcgcttgct gatccattca tagccgctgt agcttttcgg aattccgtgc aggtagtcga
     4501 cttcggcatg atacaactcg ccgatagttc catccgccaa aatcgttttg atagtttcat
     4561 acagaggatt ccagcgcagg acaaaactga cgacacttct cacgttgttc tcgcgcacgg
     4621 cggtttcaag tttgcgaaga ccgtctaaat ccagcgcaat gggcttctcc accaccacat
     4681 gcttgcccgc ctgtgcgcac gccacgccct gttccacgtg caggtggtgc ggcgtacaga
     4741 tggacaccat gtgaatatcc gaggatttca gcaactcctc gacatcggtg taggcgcggc
     4801 aatttacgag tccgtactcg cgcgccttgg cttccgcttt cgctttgtcg cggctcaaaa
     4861 tggcgcgcac ctcggtatga ggattctgtt gataggcttt gatgtgctca cccgatacgt
     4921 cgcccgttcc aagaacggcc gctccgaatt gtttcatccc tccaccttcc agctttgaaa
     4981 acggcggttc agaaggtccg gcttcggttc cccgctgaac gccagaacgc cccagcgaaa
     5041 ccggatgggg cggttcaccg caagatcgaa cggcgcgcga aaagtaggcg cggcgctcat
     5101 atacccgaac gccttattca tgacgaaaaa ggcgttcgga tgccggggat tttgaggatg
     5161 atcgaacatg gcgacccctg ctgtccccgt ggcatcgagt ttgccgtaat aggcacacca
     5221 ttgcgcggcg tccccgttgg ccttctcaat cgtattcgtt ccattggaat tgagcacccc
     5281 tccgccgtcc atgctgtgca caaaccggat ccccagcccg ttatacacat gttgcccggc
     5341 ggcaagtttc accggttccg aaggtgcttt caattccgtc gtccaatcca cccacacgcc
     5401 ctgttcccac ggaggaggca ctttcagcgt gcgcttttcg atcagaagca gcttgccctc
     5461 cgccacccag tgattgatgg aaacgatttc ggcggcggcg ccgacttgct tgcgctcaaa
     5521 gcgctgatgc acgatttgtc cgtgccttgc ggggttggtc tcaccccaga agtcgatgcc
     5581 gttgacctct gaccatgcca ccatcaatcc gcggtggtgt atatggtcct ccggggcatc
     5641 caaagtcagc ggctcgcctc cggcgaggca taggggatgc acgtaaggtt tcggacggcc
     5701 gctgctatag cgatattcga gcagcgtttt gcccccatac gataccgcca gcttctcacc
     5761 cttcgtatcg gagagacgca gcgggccgcc cgccagggca aaccctgctg cgcataatat
     5821 ttcccgtcgc gtcgtcatat caggccgcct cttccatacg gtttaaaacg atgctatcag
     5881 catagtgcaa tcggatctga tagaccataa taaagcagtc ggatactatg cctaagccgg
     5941 ccggaaagta aagatgaacg gataccagag aatcgccgct gttctcggag gaaaacctcc
     6001 tgaccgtgtg ccggtgatgc tgcacaattt cctgatggcg gcgcgcgaag ccggtgtctg
     6061 catgcgtgag taccggcgca gcccgcgggc gatcgcgcgt tgctttatcg agtcggtgga
     6121 gcgctacggc tatgacggcg ttgttgtcga cgtggacacc gccaccctcg cgggtgctgt
     6181 gggcgttccg gtcgactacc ccgacgatgc gcctgcccgc tgcagcgggg aagggaaaat
     6241 aggcagcctc gaagaagtcg cggaccttac tccccccaac ttgcaaaagg atgccggtat
     6301 cgaaacgtgg ctcgaagcaa cggctattct caaacgtcac ttcggcgacg aagtgtatat
     6361 ccgtggcaac tgcgaccagg ctccgtattc gctggctgct tccatgcggt cgccggagga
     6421 gtggatgatg gacctgatgg acgaagcgaa ccatgagcgc gcacataagc tgctcgcctg
     6481 gtgcaccggg gcggtttcgg aattcatccg gctgatggct gccaccggcg cccacatggt
     6541 ttccaatggc gatagctggg ggagcccgga cctggtgtcg ccccgcctct accgcgaatt
     6601 tgcgctgccg catgaaaaga gtgtcgtcag cgcggcgcat gtggcgggca aaccttactt
     6661 cctgcacatt tgtggaaaaa cagaccgtat ccttccggac atggtttcaa ccggcgcgga
     6721 cgggctcgag atcgactaca agacggatgt acgcctggcg catgatactc tgcgcagcac
     6781 aaccgtcttc atcggtaatc tcgaccccag tggcgtgctc gccaacggca cagccgcgct
     6841 ggtggaagaa aagacccggg agttgttgcg agtgttcagc gatacgccgc gcttcatact
     6901 caatgccggt tgcgccatcc ctgccgatac gccaccggat aatataaagg cgatgattca
     6961 gacagccagg gaacaccggc caatgtagct ataaggactc accatgaaaa ccacatccgc
     7021 gctaaatgct ttatctcgcc gcgacgtgct tcaggcggcg gtctccgccg cgctggcggc
     7081 gcctgccgtg gtgcccgcgt ccgcgctcgg cctcggcggc cagacgccac ccagcgaccg
     7141 tatcagcctg ggaattatcg gcgtcaatgg catgggccgg tcaaatctgg ccaactgtgc
     7201 caagcacccg gacgtcgttg ttaccggtat ctgcgatgtc tccacggacc gccgcgaaaa
     7261 ggctctcgaa ctgcatcgcg ccacagccaa aggctacaac gattaccgcg agctgctggc
     7321 gcgaaaagac attgatgcgg tgattattgc ctcttccccc cactggcacg cattgatggc
     7381 aattgatgca gtgaaggcgg gcaaggacat ctatctgcaa aagcccatga cgctctatcc
     7441 ggacgaaacg ctggccgtgc ggaacgccgt caaccggcac aagcgcatca gccagatcgg
     7501 tacgcagatt catgcgggct cgaattaccg gcgtgtggtc gaatgggtcc ggtcgggaaa
     7561 actgggtccc atcagtgtcg tgcgcacttt caacgtgatg aaccagggcg tcaatggaat
     7621 cggtcatgcc cccaaagagg acgcgccgaa agggctggat tgggggatgt ggctgggccc
     7681 cgccgccgag cgtcctttca atgcgctgct ctacgccgac agctacaatc actgctcttt
     7741 ctgggactac tcgttcggct ggacgcccgg catggcgccg cacattatcg atctccccat
     7801 ctgggcgttg aatctcggcg tgcccgaagt caccagttgc acgggtgggc gctacgtgat
     7861 ccgcgacgac ggcgacgcac ccgacacaca ggaagcgacg tggcgctatc cgaattttac
     7921 gatgacctgg atgatgaact gcgccaacag cttcgctttc gatttcggtc gcggcaagcc
     7981 cgcgcggcgc ctgggcatct atttccacgg tgtgaacgca accctgttca cggattacgg
     8041 aaaacacgag atcgttccgg aaggcgactt cctgaaagac tctacgccgc caccggaatc
     8101 gattgccgca tcacccggcc atgagcgcga gtggctggat tcggttaagt cccgcaagga
     8161 gccgagttgc agcgtcaatt atcattacaa agtcgatctg gcgctgacgc tcgcgaacct
     8221 ctcttatcga ttggggcgtt ccgttcgctt tgatcccaag atggaaaaga ttgttggcga
     8281 taaggaagcg gtgaagctgg cgaggcccac ttaccggcat ccgtggaagt tcccggcgga
     8341 atatttataa gccggcaagc gggccaaggg ttcagttccc ggcgatctga attttgccga
     8401 gtggatacca gccctctgta tccgctcctg ctatcgcgag ctttactgcc ggcacgcgtg
     8461 tttgcggatc cagaatcccg atggcgagtt catattcgcc tcccggcatt cccgcgggaa
     8521 cgaagacggc gttgtcatac aaattgtcgc ccggaagcca cgtgcggata tcggcgtctg
     8581 ttagcagcac ctcggttttt tgcgttccct tcaagcgaag agccagcggg aattccttgt
     8641 aaattggcgc gacgcctttg ttctcccacc acgtcgtaaa cgcgatcttc ccctgtgagc
     8701 tcacggcagc cggataggtg aattttcgca gcacgaaccg gtaacccatc ttcttgagcc
     8761 agcggttcac ctgcggccac cactcatccg gcacggcgga agattttccg ttgaatgagg
     8821 agatgtgcca cttcagcgac tggtcgatga tgtaatccac gtcccatccc ttgttcttcc
     8881 agtgctgcat cacccagcag acttccagcg acaccggcgc tttcttccat gcatcctcca
     8941 tcccgaaatt gatgatggcc tgcggataat agtcctgcat gtgcgaccag ttggggttat
     9001 tgaagccgcc catgtcgcca agacaatcca cgcgccagcc gacgggcttc ttcgagatcc
     9061 cgtatttatt cgtcttctca tcggtcagca gcatcaccag cggtgttttc gaaaaagatt
     9121 ccacgtagct gtccaccagg gcctgccgtg taggctcagt caagtccacg gcgccggcgc
     9181 cttcgcccca
//
LOCUS       DASTMK010000571         4600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_285493, whole genome shotgun sequence.
ACCESSION   DASTMK010000571 DASTMK010000000
VERSION     DASTMK010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285493"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFQ79_23415"
     CDS             complement(<1..746)
                     /locus_tag="VFQ79_23415"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0142692.1"
                     /translation="MLNSIIDFHLRHRFFVLAGLLGLVGYGVYTMLQIPIDAFPDLTN
                     NQVVVTTEAPAMAPVEVEQLVTFPIETALMGLPKTEGIRSISKLGLSMVTVVFDDSVN
                     TYFARQLVNERIQEVRARLPEGLEPTLGPVATAFGEVYQYTLEGNGMSAMELKTLHEW
                     QIKNQLRTVVGVNEVNTWGGESKQYQIEVDPVRLQSYGLTLHDVFLRVQENNANFGGG
                     FIEHYAEQYTVRGLGRTQSIGDVSNIVVLAR"
     gene            complement(749..1951)
                     /locus_tag="VFQ79_23420"
     CDS             complement(749..1951)
                     /locus_tag="VFQ79_23420"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0142693.1"
                     /translation="MKYAFVLLLALTVGCSQKEADTVQARAPERRAAPPRHVGMDAAT
                     QERAGITIEVVRPLPAPQVVRATGRITTNENRTWRVGAITEGRIIRVYANPGDRVEQG
                     QVLARMHSHDIHESRALYRKALTDSVMLKSAESLAKRARDRYRRLYELKAASLEQLEK
                     SEGELKSAQTAVANAEIEVNRTRLHLMEFLGVSLDASHDHQAGDEHDEDLIPIRSPAD
                     GTVLVRSVTPGTVVGISGETFVVSDLTKLWTIAAVNEENLSRLRTGARARVSVQAYPD
                     RTFPGVVTKIDEELDATTRTIRARIELANSSGRLKPEMYANAEIDAGSSEPALFVPQS
                     ALQEVSGEQVVFVRTGPEEFELRPVVTGRILDARLEVTGGLEPGDEVVSRGSFLLKSQ
                     LLKSSLEE"
     gene            complement(1948..3171)
                     /locus_tag="VFQ79_23425"
     CDS             complement(1948..3171)
                     /locus_tag="VFQ79_23425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0142694.1"
                     /translation="MMRWFSILAVVCTAAVACLAEPLSIRQAVDKALSSHPLLELESQ
                     RVLEAEGYHLQAGLRPNPLFVFQVEGLRAHGNPGFRFGHDADTFAYLQQPIETGGKRG
                     RRVDLAEQRRRLAELGKELATRRIVNRVRLAYWAASGAQRIRDLLIQDSNNFEKIVEY
                     HRNRVQEGAMAEVDLIRIQIEAGRLELSANSAALEADRARIRLFESMGQSSFPPVQLS
                     EPLDSDYKTPAYDLPEAVDRRVEVRIAQRAVEQARTNERLQGAMSAPDVQALLGYKRG
                     TGFNTVITGVQVDLPFWSRNQGNIAAARAGVRGAESTLASTRAQVQAEIVAAQADYDI
                     RRRQISQYLKPLRERARESSRIALEAYREGGADLLRLLDAERARIDAELLYFQTLTEF
                     QRSAASLEDAIGVLP"
     gene            complement(3200..4483)
                     /gene="dinB"
                     /locus_tag="VFQ79_23430"
     CDS             complement(3200..4483)
                     /gene="dinB"
                     /locus_tag="VFQ79_23430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU0142695.1"
                     /translation="MKSIFHLDMDAFFVSVEELFDPSLKGKPVVVGGQRDQRGVVSAA
                     SYAARRFGVHSAMPLRTAAKLCPQAIFVDGHPHRYREYSHKVYEVLQSFTPRVEMASI
                     DEAYLDLTGAGRLHGPPLRAAHELHNRIHRATNLNCSIGAATSRLVAKVASDQAKPNG
                     VLWILPGQEARFLAPLEIRKIPGVGKVTEKNLHAIGVTKVGDLAGLDEAFLERRFGKL
                     GLALAGKAHGLDAGGWFDGEIGDATNPKSISHEHTFNEDTADAIALEAMLARLSEMVG
                     RRLREHALHARTVQLKLRYSDFSTFTRAHTLDHATKLDNEILDHVRRLFRKAWTGAAI
                     RLIGVHVSSFEHSEGQLSLLDGEKNARWSKALSAADRIRDRFGESSISLAGGLKSAFR
                     ERVHENPASLPGKTPKTGKDDTTVPPAERNESSGK"
BASE COUNT          848 a         1446 c         1339 g          967 t
ORIGIN      
        1 cgcgccagga cgacgatgtt ggacacgtcc ccaatactct gtgtgcggcc aagccccctg
       61 acggtgtact gctccgcgta atgctcgatg aagccgccgc caaagttcgc attgttttcc
      121 tggacccgga ggaatacgtc atgaagagtc agcccgtagc tttgaagccg tacgggatcg
      181 acttcgatct gatattgctt gctctcgccg ccccacgtgt tcacctcatt aacacccaca
      241 acggtccgca actgattctt gatctgccat tcatggaggg tcttcaattc catggcggac
      301 atgccgttgc cttcgagcgt gtactggtaa acctcgccga aggctgtcgc gacaggtccc
      361 agcgtgggct ccagtccttc gggcagacgg gcccgtactt cctgaatccg ttcgttcacc
      421 agttgtcttg cgaaataggt gtttaccgag tcgtcgaaga caaccgtcac catggacaaa
      481 cccagcttcg agatggaacg tatgccttcc gtcttgggca agcccatgag ggccgtctcg
      541 atcgggaatg tgaccagttg ctccacttcc acgggcgcca tcgccggagc ttcagtggtc
      601 acgaccacct ggttgtttgt cagatcgggg aaagcatcga tcggaatttg cagcatcgtg
      661 tagacgccgt aaccaaccag gccgagcagg ccggccaata caaagaaacg gtgccgcaga
      721 tgaaaatcga tgatggagtt gagcaatcct attcctccag gctggacttc agcaattgcg
      781 atttcaggag gaaactgccg cggctgacta cttcatcgcc gggttcgaga ccgccggtga
      841 cctccagacg ggcgtctaag atgcggccgg tgacgaccgg tctaagctcg aattcctcgg
      901 gaccggtgcg cacgaaaacc acctgctccc cgcttacttc ctgcaacgcc gattggggca
      961 cgaatagcgc cggttccgaa ctgccggcat cgatttcggc gttcgcatac atttccggct
     1021 tcagcctgcc gctcgagttg gccaactcga tgcgcgcacg gatggtgcgc gtggtagcgt
     1081 ccaattcttc gtcgatcttg gtcaccactc ccggaaacgt gcggtcgggg taggcctgca
     1141 cggagacgcg ggcacgtgcg ccagtgcgca ggcgcgacag gttttcttca ttgacggcgg
     1201 caattgtcca cagttttgtg agatcgctga cgacaaacgt ctcgccgctg atgccgacaa
     1261 ccgtgcccgg agtcacactt cgaacaagaa cggttccatc cgccggcgaa cggatgggaa
     1321 tcaaatcttc gtcgtgctca tcgccggcct ggtgatcgtg gctggcatcc aatgacaccc
     1381 cgaggaactc catcaggtgc aatctggtcc ggttgacctc gatttcggcg ttggcgacag
     1441 ccgtctgggc gctcttcagt tctccttcgg atttctccag ttgttcgagc gaggcggcct
     1501 tgagctcata caagcgtctg tagcggtccc ttgcgcgctt tgcgagcgat tccgcggatt
     1561 tcagcatcac tgaatccgtc agcgcctttc tgtaaagggc gcgagattcg tgaatatcgt
     1621 ggctgtgcat gcgtgcaagc acctgtccct gctcgacgcg atcaccgggg ttggcatata
     1681 ccctgatgat gcggccctcg gtgatggctc ctacgcgcca ggtgcgattc tcgttggtgg
     1741 tgatgcggcc ggttgcgcgt acaacctggg gcgccggcag cggccgtact acttctatcg
     1801 ttattcccgc gcgctcttgg gtagcggcgt ccatccccac gtgcctcggc ggcgccgccc
     1861 gccgctccgg cgcacgcgcc tgtacagtgt ccgcctcttt ttgcgaacag cccacggtga
     1921 gagccagcaa taaaacgaat gcgtacttca tggcaatact ccgatcgcat cctccagcga
     1981 agcggcgctc cgctgaaact ccgtcagtgt ctggaaatag agcagttccg cgtcgatccg
     2041 cgctctttcg gcgtccagca gtctcagcag gtccgcgcct ccttcccgat acgcctccaa
     2101 ggcgatccgg gacgattcgc gggctcgttc cctcagtggt ttcaggtatt gcgagatctg
     2161 ccgccgccgg atatcgtagt ccgcttgggc ggccacgatt tccgcttgca cctgcgccct
     2221 ggtcgaagcc agagtggatt ccgccccgcg cacccccgcg cgtgcggcgg cgatgttgcc
     2281 ctgattgcgg ctccaaaagg gcaagtccac ttgcacgccg gtgatgaccg tgttgaaacc
     2341 cgtaccgcgt ttgtagccca acagcgcctg cacgtccggc gcggacatgg ccccctgtag
     2401 gcgctcgttt gtgcgcgcct gttcgaccgc ccgttgcgcg atgcggacct cgactcgccg
     2461 gtccacggct tccggcaaat cgtaagcggg tgtcttgtag tccgaatcca ggggctcgga
     2521 aagttgaacc ggcggaaacg aagactgccc catggattcg aacagcctga tacgcgcgcg
     2581 gtcggcttcg agtgcggcgg aattcgctga aagttccaga cgccccgctt caatctggat
     2641 tcgaatgagg tccacttccg ccatggcgcc ttcctggacg cgattgcggt ggtactcgac
     2701 gatcttctcg aaattattgc tgtcctgtat cagaaggtct cgaatccgct gcgctcccga
     2761 agccgcccag taagccagcc ggacgcggtt gacgatccgc ctggttgcca actccttgcc
     2821 gagttcggcc aatcgccgtc tctgttccgc cagatccaca cgcctgccgc gcttgccgcc
     2881 cgtttcgatc ggctgctgaa gataggcgaa ggtatcggca tcgtggccaa agcgaaaccc
     2941 ggggttgcca tgcgcccgca ggccctccac ttgaaacaca aaaagcggat ttggacgcag
     3001 gcctgcctgt agatggtacc cctcggcctc gagaacgcgc tgcgattcca attccagcag
     3061 aggatgcgag gagagcgctt tatcgacggc ttgtcgaatg cttagcggct ccgcgaggca
     3121 cgcaaccgcg gcagtgcaaa caaccgcaag aatggagaac catcgcatca taaattccga
     3181 aatcccaatt ttcaatctat cacttgccac ttgactcatt tctctccgcc ggtggtacgg
     3241 tcgtgtcgtc ttttccggtt tttggcgtct tgcccggcag gctggccgga ttctcgtgca
     3301 cccgttcgcg gaaagcgctt ttcaggccgc cggcgagcga aatgctcgat tccccgaaac
     3361 ggtcgcggat ccggtcggct gccgaaagag ctttgctcca gcgcgcgttc ttctctccgt
     3421 cgagaagact gagctgccct tccgaatgct caaacgaaga aacatgcacg ccgatcagcc
     3481 ggatcgccgc cccggtccag gctttccgga acagccgccg cacgtggtcc agaatttcgt
     3541 tgtcgagctt ggtggcgtga tccagcgtat gcgcgcgcgt gaatgtgctg aaatcggagt
     3601 agcgcaattt caactgcacc gtccgggcgt gcagtgcgtg ctcgcgcaga cgccggccca
     3661 ccatttccga cagccgtgcc agcatcgcct ccagtgcgat cgcgtccgcc gtatcctcgt
     3721 tgaaggtgtg ctcatggctg atggatttcg gattggtcgc atcgccgatt tcgccgtcga
     3781 accagccgcc cgcgtcgaga ccgtgcgcct tgcccgccag cgcgagcccc aatttgccga
     3841 agcgccgctc gaggaaagcc tcatccaggc cggcaagatc gccgaccttt gtcacgccga
     3901 tggcgtgcag gtttttctcc gtgacctttc ccacgccggg aattttgcgg atctcgagcg
     3961 gcgcgagaaa ccgcgcttcc tgtcctggta agatccaaag cactccgttc ggcttcgcct
     4021 ggtcggaggc gaccttcgca accaggcgcg atgtggccgc gccaatggag caattgagat
     4081 tcgtggcgcg gtggatgcgg ttgtgcaact cgtgcgccgc acgaagcggc ggaccatgca
     4141 gacgcccggc gccggtgagg tccagatagg cctcgtcgat agaagccatc tcgacgcgcg
     4201 gcgtgaacga ttgcagtact tcatagacct tgtgcgaata ctcgcggtaa cggtgcgggt
     4261 gtccgtccac gaaaatcgcc tgcgggcaga gtttggcggc ggtacgcagc ggcatcgccg
     4321 aatgcacacc aaagcggcgc gcggcataag aggcagccga tacgacgccg cgctgatccc
     4381 gttgcccgcc gactacgacc ggtttgcctt tcaaggatgg gtcgaacagt tcttccaccg
     4441 agacgaagaa tgcatccatg tccaggtgga agatcgattt cacggaaccg tctccaggcg
     4501 ccgcaatact tcgagaacga cttttcctac aatctcgaag ctgttggcgc tcagcttgtc
     4561 catcgtgtca cgttcggtgt gccagtagct ttccccaccc
//
LOCUS       DASTMK010000572         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_253144, whole genome shotgun sequence.
ACCESSION   DASTMK010000572 DASTMK010000000
VERSION     DASTMK010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253144"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFQ79_23435"
     CDS             <1..305
                     /locus_tag="VFQ79_23435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0142696.1"
                     /translation="RDRFIQAVRAEGVPVSAGYGPLNKDPFLENTFASRTWRAVYTKK
                     EIDAWRERNQCPANDRLTREGLWFSQNVLLADKAAMEDIAMAFGKVHRHAEAIRKG"
     gene            379..1926
                     /locus_tag="VFQ79_23440"
     CDS             379..1926
                     /locus_tag="VFQ79_23440"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0142697.1"
                     /translation="MKHESALPQRYTEAAFALLRIVIGWHFLYEGWTKLAFPGWTSAG
                     YLKSSTGPLAPFFGWLAGDGIRTAIIDQMNIWALILIGLGLMLGLLVRPAALGGIALL
                     SLYYLAYPPFFGMSPSGASEGAYLIVNKNLVELCALLVVVACPAYSFGLDRILRRRSG
                     TAPEVPAQPAPVFSLDRLVPAYLGPVSRRQYVATLAGVPFMGAFVLAVLKKHGWKSFE
                     EVSLGGRAQRKDTFVASATMKSFQFSAISDLKGRLPMGQIGNLTLSRMILGGNLMGGW
                     AHARDLIYVSKLVKAYHHRDKIFETFAIAERCGVNTILTNPLLCDVINDYWRNGGRIQ
                     FISDCGGKDVLQMIQKSIDRGAAACYIQGGVADRLVEEKKFDVIAAGLDLIRRNGLPA
                     GIGGHKLKTVQACVEMGLRPDFWMKTLHHTNYWSAKTLPENDNIWCTEPADTVSYMAQ
                     LREPWIAFKVLAAGSIEPAVGFRYAFESGADFICVGMYDFQIVDDVNIALNVLNRSLV
                     RTRQWCA"
     gene            1927..>2643
                     /locus_tag="VFQ79_23445"
     CDS             1927..>2643
                     /locus_tag="VFQ79_23445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0142698.1"
                     /translation="MRPGSLRPRERVLLALAHRETDRVPVDFLATAETWMNVKTFLGT
                     ADEERVLQLLGVDLRHPRQPYIGPPLETYDDGTWRDAWGVTRHKVANKFGVYDEIARH
                     PLACISDARELDEYEWPQPDWWDAEGLANEIRRLDGIAEYAIALEEFGDPGGMFEIAW
                     YLRGMEQFLADLIENPDLAWELMRRVTDFYIAMLERVMHAAGERIDLIWTSDDIAHQH
                     GILMSPRMWRKLVAPHHERLN"
BASE COUNT          514 a          776 c          814 g          539 t
ORIGIN      
        1 cgcgggaccg gttcatccag gcagttcggg cggaaggagt tcccgtatcg gcgggttacg
       61 gtccgctcaa caaagatccc tttctcgaaa atacgttcgc ctcacgcacc tggcgggcgg
      121 tgtacacaaa gaaggagatt gacgcctggc gcgagcggaa ccagtgtccc gccaacgacc
      181 gtttgacccg ggagggcctc tggttcagcc aaaacgtgtt actggcggac aaagcggcga
      241 tggaggacat tgctatggca ttcggcaaag tccaccgcca cgccgaagcg atccgaaaag
      301 ggtagtacgg ccgtccggcg gtactctttt gtataatttg gtcctaaatt cgtgttagtc
      361 tggggctgag gaacccgcat gaagcacgaa tccgccctgc cccagcggta cacagaagca
      421 gctttcgcgc tgctgcgcat tgtgattgga tggcacttcc tgtatgaagg ctggacgaaa
      481 ctcgcctttc cgggctggac ttccgcaggt tatttgaaat cgagtacggg tcccctggcg
      541 ccgtttttcg gctggctggc gggagacgga atccgtacgg caatcatcga tcagatgaac
      601 atctgggccc tcatccttat cgggctcggc ctgatgctcg gtttactcgt gcgccccgcc
      661 gcgctcggcg gcatcgcgct gctcagcctc tactatcttg cgtatccgcc atttttcggg
      721 atgagcccca gcggtgccag cgaaggcgcg tatctcatcg ttaacaagaa cctggtggag
      781 ctttgtgctt tgcttgtggt tgtggcttgc ccggcctatt ccttcggcct ggatcgtatt
      841 ctgcggcggc gtagcggaac ggcgccggaa gtgccagccc agcccgcacc tgtcttcagt
      901 cttgaccggc tggtgcccgc atacctgggc ccggtttcac ggcggcaata cgtcgcgact
      961 ctcgccggtg taccgttcat gggcgctttc gtcctcgcgg tactcaagaa gcatggatgg
     1021 aaaagcttcg aagaggtatc gctcggaggg agagcacagc gcaaggatac cttcgtcgcc
     1081 agcgcgacca tgaagagctt tcagttctcc gcgatcagcg acttgaaggg gcggcttccc
     1141 atggggcaaa tcggcaacct cactttgagc cgcatgatcc ttggtggcaa cctgatgggc
     1201 ggctgggcgc atgcccgcga cctgatctac gtgtcgaagc ttgtcaaggc ctaccaccat
     1261 cgcgacaaaa tcttcgagac tttcgccatc gccgagcggt gcggcgtcaa cacgattctg
     1321 acgaaccccc tgctgtgcga cgtgatcaac gactattgga gaaacggcgg ccggattcag
     1381 ttcatatccg attgcggcgg aaaagacgtg ctgcaaatga ttcagaagtc catcgaccgt
     1441 ggcgcggcgg cttgctatat ccagggcggg gttgccgacc gcctggtcga ggagaagaag
     1501 ttcgatgtca tcgccgcggg tctggacctg atccggcgca acggtctgcc ggcgggcatt
     1561 ggcggccaca agctcaagac cgtgcaggct tgcgttgaga tgggtctgcg gccggatttc
     1621 tggatgaaaa cgctgcacca caccaattac tggtcggcga agaccctgcc cgaaaacgac
     1681 aacatctggt gcacggaacc ggccgatacc gtttcgtata tggcgcaatt gcgtgagccg
     1741 tggatcgcct tcaaagtgct ggcggccggt tccatcgaac cggcggtggg cttccgctat
     1801 gccttcgaga gcggcgccga tttcatctgc gtgggcatgt acgacttcca gattgtcgac
     1861 gacgtcaaca tcgcgctcaa cgtgctgaac cgctcgctgg tgcgtacgcg gcaatggtgc
     1921 gcgtaaatgc ggccgggcag cctgcgcccg cgcgagcgtg tgctcctcgc tctcgcgcac
     1981 cgcgagacag atagagtccc ggtcgatttt ctcgctacag ccgagacctg gatgaatgtg
     2041 aaaacgtttc tcggcacggc ggacgaggaa cgcgttctcc aacttctcgg cgttgacttg
     2101 cgtcatcccc ggcagcctta catcggccct cccctcgaaa cgtacgacga cggaacgtgg
     2161 cgcgatgcgt ggggtgtgac gcgccacaag gtggcgaaca agttcggagt gtacgacgag
     2221 atcgcccgcc accccctggc ctgtatcagc gatgcgaggg aactcgacga atatgagtgg
     2281 ccacaacccg attggtggga tgccgaaggg cttgccaatg agataaggcg gcttgatggg
     2341 atcgccgaat atgcgatcgc actcgaagag ttcggcgatc cgggcggcat gttcgagatt
     2401 gcctggtact tgcgcggcat ggagcaattc ctggcagatc tcatcgaaaa cccggacctt
     2461 gcctgggagc ttatgcgccg cgtcacggac ttctacatcg ccatgctcga acgcgtgatg
     2521 catgcggccg gagaacgcat cgatctgatc tggaccagcg acgacatcgc ccaccagcat
     2581 ggaattctga tgtcgccgcg catgtggcgc aagctcgtcg cgccgcatca tgagcgtctc
     2641 aac
//
LOCUS       DASTMK010000573        12365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_348819, whole genome shotgun sequence.
ACCESSION   DASTMK010000573 DASTMK010000000
VERSION     DASTMK010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348819"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VFQ79_23450"
     CDS             complement(<1..1592)
                     /locus_tag="VFQ79_23450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142699.1"
                     /translation="MKHGGIWAAIVLVPCLWPHEANLSGRVGDSAGLVIPEATVRCLN
                     TRAGINRITRTDEHGIYRLPSLPPGIYDIDVTAHGFRKVRRSDLPLHEGQKVILDLTL
                     EVGSVQESVTVDSGRQLVNLSTAEISSVVEGEQVERLPLNGRNVLQLTLLAGGAVDTG
                     GGLANQGFIHPSSRVYPSSSGGRADSMTFNFDGGINNDRYTNVALPVPNPDAIQEFTF
                     LVNNFSAEYGAASGGVISIVSRSGTSAYRGRVYNYLRNASLNATNFFTPGVNDRLRRN
                     QAGFTLGGPVRIPRVFDGRQHTFFFVAYQLTMLRRSPLTTNVRTLTADERNGNFSVLA
                     AQLGRPVLDPLTSQQFPDNRVPVTRLDPVMLKLASLLPVGDPTTGLLTGRVARVVVNE
                     PQWTGRVDHNRPSGDRLTLRYFRDHYAPALTADPANPYLTGNGSIRFRVQNASTTYTQ
                     VYSPRLLGVFGFTVNRSHNTEVYDYPGPANNNPAALGIQGFPSGSFFLRSPFVAVGYQ
                     GPYTILASNNFQGQGSLTYVTG"
     gene            complement(1685..3397)
                     /locus_tag="VFQ79_23455"
     CDS             complement(1685..3397)
                     /locus_tag="VFQ79_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142700.1"
                     /translation="MADASWEVKNGHPPTGVLLLYLEGELEGRLAAAVEHHTRACWTC
                     RAQCERMERGIRSFVDFHETAEIPPPPAGRQAFLSRLREQAPVATSGAAGRWFRWRRG
                     LLAAGGLSAAAMLALLVVSVLVPTHSVSAAQFLDRATRAAQATHASGSKQRIVERIRI
                     RRGSQVLHSTVHYGVGVRRTTSAPPPAEMQRALELARIDWRDPLNVSDFAAWRESLRS
                     RKDHINQEGGELVLITEAKGASPVSRASLTVDQLTWRPVARHVDFPDQPPVGVVMESR
                     EVQDDVEPAAGALPADPAPQPPEQASAPASESAAPDARQLERAELELRELFHLLAADV
                     RDAPEIWQSDGCVYFRLFAESPARREEILRATQRISLACELPTGPGGTYIPPEKTPDP
                     GSSTTWTPTKPPFEQALREHFGGQDQANRYLDQVNDLYVRLLAEVTALERLGERYPDL
                     EIRQMPPDEQARIERLARDHVVEIAVSSRDYSALASPILDSIANRDTRSTKLTPTCVP
                     WQTAAPLIAADLRRFQRSFSRLFVKDETPTPGEQSAANLLSDAAEAQELWQRHIEALC
                     ASSR"
     gene            complement(3390..3956)
                     /locus_tag="VFQ79_23460"
     CDS             complement(3390..3956)
                     /locus_tag="VFQ79_23460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0142701.1"
                     /translation="MISKSVVVPFSLESDSAGSQRSSLRIVEELYAEHYDTLYRYLIL
                     SGCQPADADDLIQSGFVRLLRFLQQGNRLEKPKPWLIRVVHNLRIDDARRERRESTVD
                     DGQLEARMTEVFGTMPDVETELLQTERRRALQKAVGSLTVRQYEFLLLRAAGLKLREI
                     AEIHQVTIQTVAEICARATERLGRIIHG"
     gene            4369..4590
                     /locus_tag="VFQ79_23465"
     CDS             4369..4590
                     /locus_tag="VFQ79_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142702.1"
                     /translation="MNASMARRIRNAETVLISAIDRQKDDRRKSTSLSETGRFPQNSP
                     GFSVLIRDEHGVAHWNRPLAGLRRPSCLG"
     gene            4607..5824
                     /locus_tag="VFQ79_23470"
     CDS             4607..5824
                     /locus_tag="VFQ79_23470"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HEU0142703.1"
                     /translation="MEPNVTAAGNGGATVSATQHGAGGGREYTEEEVRQLVAALDEPF
                     DPRDIKWRVTNMTSDRRRGQMMAYADPRAYTDRLNELFTVRGWTREYSVEAITNVERK
                     FSNGSQIAGKVVVTCRLTIHGLGAHSGIGEEWADNENAGTAAEAQAFKRACSCFGLGR
                     YLYDLEGGWVDLDDRKQPMRAPKLPDWAIPKRKQADGTASPTMGQHNGRTHDGATQPA
                     EAAILRTNIKSLSGQVGYSLSRSVLIGVAKAEDAEKIASTLLPAVASKLEDTLRGVER
                     LRRATAIVGQQRYSELCRELNFASDALDDIPDRSALRRLIEALEKEVGATASRQGIKG
                     SAAPPTAKAVSEARSQLLTEAQRVARLKGMKVAEVVDRAAKGAFAYSRLHRVTAGDLP
                     AIQAATAVLRRVL"
     gene            6006..6344
                     /locus_tag="VFQ79_23475"
     CDS             6006..6344
                     /locus_tag="VFQ79_23475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142704.1"
                     /translation="MHEVKIDSVLHLRDFGLEHLDEDTAIIGFAGKAEARLSRWLDFA
                     RGALLFLTVPDDPESGWFYIYDRARSAFYSLGLPVEGHWGGFREDEFDRLTQAFDLVS
                     LARNPRLLGS"
     gene            6405..7388
                     /locus_tag="VFQ79_23480"
     CDS             6405..7388
                     /locus_tag="VFQ79_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142705.1"
                     /translation="MDHRLHATAAMAENFSHLFVNRRAYTLQSTRPHPESGRHYYYRP
                     KARDGGPPPALSLETVRRHLAGEITLGIYAINPATQRSKWVAIDADYKRAIDDLLKLQ
                     YELRQDGVEPALEKSNRGAHLWIFFEQPALARDCRIYIYHTALRLGLPIKGAGLPEGI
                     EVFPRQDEVGPGEFGNAIRAPLGVHRGARESRGKRFWFYGADYTLEDQLAYLCRIRKV
                     TEAELQRFVEGKTIPWEFTQHAKRPDAPKRYFTSPANEFRILDYVKVHRQCGRNWIAR
                     CPSCAQANRDTSGDNLAISIEEPRKYICWAGCSREMIREALGRPIPQKRYA"
     gene            7385..8167
                     /locus_tag="VFQ79_23485"
     CDS             7385..8167
                     /locus_tag="VFQ79_23485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142706.1"
                     /translation="MSEKRNSNPAPRLTLPSAAVKRLAEAGIHCRPEIGLEYQRAASR
                     YVLRGRESGGATSELGRYVSFSADDGQRLPWLIRPDSLARNGDHAIVVAPLLVSIEMF
                     RFEHTYALLILRHTILETEKGKRPAVSSQVVFRGWQGQLPLDLAGKDKSQAGKIAPEF
                     FDRSGEPKEPPPVYVEAVQAVTKAVNCINCSHPHFLVAKPSSLSSSSWTAAPSSAPVL
                     DRREDPAAMRDGFPEIGRNAAIFAAAVEAAEESAEPAATAVA"
     gene            8288..8437
                     /locus_tag="VFQ79_23490"
     CDS             8288..8437
                     /locus_tag="VFQ79_23490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142707.1"
                     /translation="MTVEAATALGWLIIRGAFRAQLGLLGFAMEVGEAPWICRLQAPM
                     GSRKE"
     gene            8648..8986
                     /locus_tag="VFQ79_23495"
     CDS             8648..8986
                     /locus_tag="VFQ79_23495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142708.1"
                     /translation="MLAALMEKECMNGVQEQDKAEQSATVFPALIQVQGIPGVLLSAR
                     PGNRRIRLLLDHPIARNLGAFSWLLLAVGLSAGHPRMAVIVGGTVFIGLLIGLVNGAR
                     WLFGKSARHP"
     gene            complement(9576..10115)
                     /locus_tag="VFQ79_23500"
     CDS             complement(9576..10115)
                     /locus_tag="VFQ79_23500"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0142709.1"
                     /translation="MTDEIEQLLKNLKLGRMRSIYDEQLRAAGKQQVSYSEFVAALLR
                     SQWHHRQESALEWRIRRANLPERWSLETFPYSRQPGVKRKQIQAFAELDFVAKHENLV
                     FTGPTGVGKTGLACGILLKALQNGLRCQFVRAQDLFDEMYASLADRSTRKLIHRLARL
                     DVLLIPYTTEQTQHADRRG"
     gene            complement(10108..11502)
                     /gene="istA"
                     /locus_tag="VFQ79_23505"
     CDS             complement(10108..11502)
                     /gene="istA"
                     /locus_tag="VFQ79_23505"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEU0142710.1"
                     /translation="MLSQAQRATILELRGQGVGKREIARLLKVSRQSVRQVLQANSSA
                     VPQIERAEKATPFRQQILELLPQCKGNLVRVHEELAATGATFSYQALTAFCRREGIGQ
                     TPVVPAGRYEFAPGEETQHDTSPHEVIVGGRKFKAQTASATLCYSRMLFFELLPAFQR
                     FDCKVFLTDAVRYMNGATERVMIDNTHVVVLRGSGRAMEPVPEMAAFAERLGFRFVAH
                     EIGDANRSARVERPFHFIENNFLAGRTFADWQDLNHQARQWCDRVNATYKKHIRAVPR
                     ELYAVERPLLKPLPAWLPEVYRLHHRLVDVEGYVSLHSNRYSVPPSFIGRQVEVRETR
                     DHVEIELDARHIVRHRRIATPDQVRVMLPEHRPPRGQGPCKRDPHPEETALLAEAPEI
                     AVYIENLKCRGRKVTALLLRQLLRMLRDYPREAFLGAIEEAGRYGLYDLDRVERMILR
                     RVTREYFRLEDTDD"
     gene            complement(11927..12325)
                     /locus_tag="VFQ79_23510"
                     /pseudo
     CDS             complement(11927..12325)
                     /locus_tag="VFQ79_23510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
BASE COUNT         2346 a         3779 c         3962 g         2278 t
ORIGIN      
        1 ctgcccgtga cgtaggtcag gctgccttgg ccttggaagt tgttcgatgc caggatcgtg
       61 tagggtccct ggtagccaac tgcgacgaag ggcgagcgca gaaaaaagga tccgctggga
      121 aagccctgga tgccgagggc ggctggattg ttgttggccg gacccggata atcatagacc
      181 tcggtgttat ggctgcggtt gacggtaaag ccgaagacgc ctagcagacg cggcgagtat
      241 acctgcgtgt atgtcgtgct cgcgttctgc acgcgaaagc ggatggagcc attgccggtg
      301 agatacggat tcgccggatc cgcggtcagc gccggcgcgt agtggtcgcg gaagtagcgc
      361 agggtcaggc gatcgccgga tgggcggttg tgatcgaccc gtcccgtcca ctgcggctca
      421 ttcaccacca ctctggctac tctgccggtc agcagtcccg tcgtcggatc tcccacgggc
      481 aaaaggctcg ccagcttcaa catgaccgga tccagccgcg tgacgggcac acgattgtcc
      541 gggaactgct gtgatgtgag tgggtccagc accggccggc ccaactgggc agcgagcacg
      601 ctgaagtttc cgttacgctc gtctgccgtc agcgtgcgga cgttggtggt cagcggcgat
      661 cgccgcagca tcgtgagctg gtaagcaacg aagaagaacg tgtgctggcg tccgtcgaac
      721 acacgcggaa tgcgcaccgg cccgcccaag gtgaagccgg cctggttgcg ccgcagccgg
      781 tcgttgacgc cgggtgtgaa gaagttggtc gcattcaggc tggcgttgcg gagatagttg
      841 tacacccgcc cacggtaggc gctggttccc gagcggctga cgatgctgat cacacctccg
      901 gaggcggcac cgtactcggc gctgaagttg ttcaccagga acgtgaactc ctgaatggcg
      961 tcgggattgg gaacgggaag cgccacgttg gtgtagcgat cgttgttgat gccgccgtcg
     1021 aagttgaagg tcatggaatc ggcgcggccg ccgcttgacg agggatagac gcgggagctt
     1081 ggatgaatga agccctggtt ggccagtcct ccgcccgtat ccacggcacc gccggccagt
     1141 agcgtcagtt gcagaacgtt gcgcccgttc agcgggagcc gttccacctg ctctccttcg
     1201 actaccgaac tgatctccgc cgtggagaga ttgaccaact gcctcccgct atccacggta
     1261 acggactcct gcacgctgcc cacctcgagc gtcagatcca atatcacctt ctggccctca
     1321 tgcagaggca gatcgctgcg gcgcactttt cggaagccgt gggcggtcac gtcgatgtcg
     1381 tagattccgg gaggcaggct cggcaggcgg tagatgccgt gttcgtcggt gcgtgtgatg
     1441 cgattgatcc ctgcacgcgt attcaggcac cgtacggtcg cttccgggat gactaaaccg
     1501 gcgctgtctc ccaccctgcc gctcaagttc gcttcatgcg gccagaggca tggcaccagc
     1561 acgatggccg cccagatgcc gccatgcttc attcgcacga gattctcctg cgggatctgc
     1621 gccggacgcc gtccccgcgc tgcaattgta acctgttccg gaagccgtcg ccacggcctc
     1681 cgacttatcg actagaggcg cacagggcct cgatgtgccg ctgccagagc tcctgagcct
     1741 cggcggcatc cgacagcagg ttggcggcag attgctcacc cggcgtaggc gtctcatcct
     1801 taacgaacag ccgggagaaa ctccgctgga agcggcggag atccgcagcg atcagcggcg
     1861 cggctgtctg ccaggggaca caggtaggag tgagcttcgt gctgcgtgta tccctgttcg
     1921 ctatcgagtc taggataggc gatgcaagag cggaataatc ccgggaagag acagcgatct
     1981 ctaccacgtg gtcccgcgcc agccgctcga tcctggcctg ctcatcgggt ggcatttgcc
     2041 ggatctcaag gtcaggatag cgctcgccga gccgctccag cgcggtgact tcggccagca
     2101 ggcgtacgta cagatcgttt acctggtcca gatatcgatt cgcctggtct tgaccgccga
     2161 agtgctcacg cagcgcctgt tcgaagggcg gcttggtggg ggtccaggtc gtactcgaac
     2221 ctggatccgg cgttttctcc ggcgggatat aggttcctcc tggtcctgtt gggagttcgc
     2281 acgccaagga gatgcgttgc gtcgcgcgca gaatctcctc gcgacgcgca ggggattcgg
     2341 cgaacaggcg gaaatagacg caaccgtcgc tctgccagat ctcgggcgcg tcccgcacgt
     2401 cggccgcgag caagtggaac agttcgcgca gttccaactc cgctcgctcc aactgtcggg
     2461 catcgggagc agcagactcg ctagctggcg cactcgcctg ctcaggcggc tgcggcgcag
     2521 ggtcggcagg aagagcgcca gcggcaggct ccacgtcatc ctgaacctca cgggactcca
     2581 tcaccacacc tacgggtggc tgatcaggaa aatcaacgtg gcgcgcaacg ggccgccagg
     2641 ttaactgatc gacggtgagg gacgcccggg acaccggaga ggcacctttc gcttcggtga
     2701 taagcacaag ctcgccgcct tcctggttga tgtgatcttt tcgggagcgc agagattccc
     2761 tccaggcagc gaaatcgctg acgttgagcg gatcgcgcca gtcgatgcga gccagttcca
     2821 aagccctctg catctccgca ggaggtggtg cggaggtagt ccgccggaca cccacgccat
     2881 agtgcacggt gctgtgtagc acctggctgc cccggcgaat gcgtattctc tcaacgattc
     2941 tctgtttcga cccggaggcg tgggtcgcct gcgcggctcg cgtggcccga tccaggaact
     3001 gcgccgccga gacgctgtgg gtcggaacca agacggaaac caccaggagg gccagcatcg
     3061 cggcggcaga cagacccccg gcagccagaa ggccgcgccg ccaacggaac cagcggccgg
     3121 ctgcgccgga ggtggcgacc ggcgcttgct cccgcaggcg cgagaggaag gcttgccttc
     3181 cggcaggcgg cgggggaatc tcagcggttt catggaagtc cacaaaggag cgaatgccgc
     3241 gctccatgcg ctcgcattgt gcgcggcacg tccagcaagc gcgggtgtgg tgctccacgg
     3301 cagcggccag ccggccctcc aactcccctt ccagatagag caggagcacg ccggtaggcg
     3361 ggtgcccgtt ctttacttcc cacgatgcgt cagccatgaa tgatccgtcc caaccgctcc
     3421 gttgcgcggg cgcatatctc ggctacggtt tgaatcgtca cctgatgtat ctccgcgatt
     3481 tcgcgcaact tcaatcccgc cgcgcgcaga agcaggaact cgtattgccg gaccgtcaga
     3541 gacccgacgg ccttctgcag cgcccgccgc cgttcggtct gcagcaactc cgtctccacg
     3601 tccggcatgg tgccgaagac ctccgtcatg cgtgcctcca attgtccgtc gtctacggtc
     3661 gattcacgcc gctcgcgccg cgcgtcatcg atccggagat tgtgcacgac gcggatgagc
     3721 cagggcttcg gcttctccag ccggttgccc tgctgcagga accggagcag gcgcacgaaa
     3781 ccgctttgga tcagatcgtc tgcgtcggca ggctggcagc cgctcaggat gaggtatcgg
     3841 tacagagtat cgtaatgctc cgcgtacaac tcctcgacga tccggaggga actccgttga
     3901 ctgccggcac tgtcactctc caatgaaaag ggcacaacta cgcttttgga gatcaaatcg
     3961 atactccttc acgtctccgt gggggtacac gaacagacca cggacagact accaccttct
     4021 atagagacga aaccgggcgc cggaattcag gtggtgggct cagccttttt tccagataat
     4081 cgctggcggt gttgtagtcc acgttcgggc tcgccactgg gagctccttc ctgggatcca
     4141 caccagatag ccgggattaa acgagcacca cggtgcagcg ccctgtgcca aagttctgca
     4201 agttctcggc attaagccgg cagaaatcgg cagttcgtac tggaccacat acccccacct
     4261 cccagaccgg tggtcgtctc aggcaaaccc gagaatattt gcctcccaga gccgccgatt
     4321 acggccatat tcatttcaat ttcaattcgc acaacaagga gaactgacat gaacgcatcg
     4381 atggcgagac gcattcgtaa cgccgaaacc gtcctgattt ccgcgatcga ccgccaaaag
     4441 gacgatcggc gcaaatcgac cagcctatcc gaaacgggtc gattccctca gaacagcccc
     4501 ggtttctcgg tcctgattcg ggacgagcac ggggttgcgc attggaatcg gcccctggcc
     4561 ggcctgcgca gacccagttg cctgggttga ggcaaggagg agaagaatgg aacccaacgt
     4621 aacagctgcg gggaacggag gcgccaccgt ttccgccaca cagcatggcg ccggcggtgg
     4681 aagggagtat accgaagaag aagtgcgcca actggtggcg gcactcgatg aaccgttcga
     4741 tccgcgcgac atcaagtggc gcgtcacgaa catgaccagc gaccggcgcc ggggccaaat
     4801 gatggcgtac gccgaccctc gcgcatacac tgaccggctg aacgagctgt tcaccgtgcg
     4861 cggctggaca cgggagtatt ccgtcgaggc gataaccaat gtcgaacgga aattcagcaa
     4921 cggttcgcaa atagccggca aggtcgtggt gacatgccgg ctgacgatcc acggtctggg
     4981 cgctcactca ggcatcgggg aggaatgggc cgataacgag aatgccggaa ctgccgcgga
     5041 agcacaggcg ttcaagaggg cctgctcgtg tttcggcctg gggcggtatc tatacgacct
     5101 ggagggcggt tgggttgacc tggacgacag aaagcaaccg atgcgcgctc ctaagctgcc
     5161 agattgggca attccgaagc ggaagcaagc cgatggcact gcatcgccta cgatgggcca
     5221 gcataatggc cggacccatg atggcgccac tcagcctgcc gaggccgcga tactccggac
     5281 gaatatcaaa tcgctcagcg ggcaggtcgg ctacagcctg tcgcgttccg ttctgatagg
     5341 agtcgcgaaa gcggaagacg cggagaagat cgcatcgacg ctgctcccgg cagtggcatc
     5401 gaagctggaa gacacccttc gcggcgtgga acgtttgcgg agggcaacgg ccattgtggg
     5461 gcagcagcgg tactcggagc tgtgccgcga actcaatttt gcaagcgacg cgctggacga
     5521 cattccagac cgcagcgcgc tacggcggct gatcgaggcg ctggagaagg aagtcggggc
     5581 cactgcgagc cggcaaggca tcaagggctc cgcggcaccg ccaacggcaa aggcggtaag
     5641 cgaagctcgc agccaactgc tcacggaggc acagcgcgtt gcccggctga aaggcatgaa
     5701 ggttgccgaa gtggttgacc gggcagcgaa gggcgccttt gcgtattcga gacttcatcg
     5761 ggtgacagcc ggggatctac cggcgatcca ggcagccact gccgtgcttc gcagggtgtt
     5821 gtaatctcga ctcccgcagc ttgagtcctt ttataaagga tgcaacgtcg ccgccgactt
     5881 cctcgccaga ctcttctcct ccatcaattc tcgttgttgt tttcccacaa accgttctgc
     5941 gggctcgtat tcttgagcgc cctcagagcc aaactgttga cagcgtaagc aaaggagctt
     6001 ctgcaatgca tgaggtgaaa attgacagcg tcctgcacct gcgggatttc ggtttggagc
     6061 acctcgatga ggacacggcc atcattggct tcgccgggaa agccgaggcg aggttgtcca
     6121 gatggctcga cttcgcgagg ggtgcgctcc tgttcctgac ggttccggac gatccggaat
     6181 cgggctggtt ctacatctat gatcgggcgc ggtcggcctt ctactcgctc ggcctgccag
     6241 tggaaggtca ctggggcggc tttcgcgaag acgaatttga ccgcctgacc caagccttcg
     6301 atttggtgtc acttgcgcgg aacccccggc tcttggggag ttgaggccgg gtggcttttg
     6361 gagcaatcgg agagatgctc tatacctctc gaaggagcgc agtgatggat cacaggcttc
     6421 acgccacggc cgcgatggcc gaaaacttca gtcatttgtt cgtgaatcgc cgcgcttaca
     6481 cgctgcaatc caccaggccg catccggaat caggcaggca ttactactac cggccgaagg
     6541 cgcgggacgg agggcctccg cccgcgctca gcctggaaac cgtgcgccgg cacctggccg
     6601 gcgaaatcac gctcggcatc tacgcgatca atccggcgac acagcggtcg aaatgggtgg
     6661 ctatcgatgc cgactacaag agagcgatcg acgacctgct caagctgcaa tacgaactcc
     6721 ggcaggatgg cgtcgaaccc gcgctcgaga aatcgaatcg cggcgctcac ctgtggatct
     6781 tcttcgagca gccggcactg gcacgggatt gccgcatcta catctaccac accgctctcc
     6841 ggctcggcct tccgatcaag ggcgccggcc tgccggaagg catcgaagta tttccgcgtc
     6901 aggatgaagt aggccctggc gaattcggaa atgcgatccg ggcgccgctc ggcgtgcatc
     6961 gtggggccag ggagagccgc ggcaaacgat tctggttcta cggcgcggac tacacgcttg
     7021 aagaccagct cgcctatctg tgccgcatcc gcaaggtgac cgaagccgaa ctgcaacggt
     7081 tcgtcgaggg caagacgatt ccttgggagt ttacacagca cgccaaacgc cctgacgccc
     7141 cgaagcggta tttcacatcg cccgccaacg agtttcggat tctcgactac gtcaaggtcc
     7201 atcggcagtg tggccggaac tggattgcgc gatgcccttc gtgcgcgcag gcgaacaggg
     7261 acacgagcgg cgacaacctc gccatctcga tcgaggaacc gaggaagtac atctgctggg
     7321 ccgggtgctc cagggagatg atccgcgaag cgctcggccg gccaattcca cagaagaggt
     7381 acgcatgagc gagaagagaa acagtaaccc cgcgccgcgg ctgacgctgc cgtcagccgc
     7441 ggtgaaacgg ctcgcggaag cgggcattca ttgcagaccg gaaatcggcc tcgagtatca
     7501 gcgggccgcc agccggtacg tgcttcgagg ccgggagtcc ggcggcgcca ccagtgaact
     7561 ggggcggtat gtgagctttt ccgcggatga tggccagcgc ttgccgtggc tgatccggcc
     7621 ggactcgctg gcgagaaacg gcgatcacgc gattgttgtc gcgccgctac tggtcagtat
     7681 cgagatgttc cgcttcgagc acacctacgc gctcctgatc ctgcgacaca cgattctcga
     7741 aactgagaag ggaaagcggc ctgctgtatc ttcgcaagtc gtctttcgtg gatggcaggg
     7801 ccagctcccc ctggatcttg ccggcaagga taagtcccag gcgggaaaga tcgcgcccga
     7861 gtttttcgac cgctccggcg agccgaagga gcctcctccc gtttacgtcg aagcagtgca
     7921 agcggtcacc aaagcggtga actgtatcaa ttgttcgcac ccgcatttcc tggttgcaaa
     7981 accatcgtcg ttgtcatcgt cgtcgtggac ggctgcgcct tcatccgcac ctgtgcttga
     8041 ccggagggag gatcctgccg cgatgcgaga cggattcccg gagatcggcc gcaatgcggc
     8101 gatcttcgcg gcagcggtag aggcagcgga agagagtgcg gaaccagcgg ccaccgccgt
     8161 ggcataggca gggtatggag cgccggtatg gaaatagacc aagtcgaact ccggagcgct
     8221 tcgctcaaag ccagcgcggc catcgcaaaa ggcctcagcg taacaacgct tctattacga
     8281 gactcctttg accgttgaag ccgccaccgc actcggatgg ctgattatcc gcggggcatt
     8341 ccgcgcgcaa ctcgggctgc tcggctttgc gatggaggtc ggcgaagcgc cttggatctg
     8401 ccgcttacag gcgccaatgg gcagcagaaa ggaataggag gagatgtcgg gcaagaatcg
     8461 gacacctgaa gatcatcgct tcgccgtaga ggacttgctt gacctctaca cgagcgggca
     8521 caactcaaac ggtgcaggcg tgaagcgcga aagcaccgga tgccaagagg ccgaaactga
     8581 acagccggcg ggtgcgtcag cttcgccgga acagaccaaa caatcagccg caactggatc
     8641 cggatagatg ctggcggcgc tcatggagaa agaatgcatg aatggcgttc aggaacagga
     8701 caaagcggaa caatcggcga ccgtgtttcc cgcgttgatc caggtgcagg gaatacccgg
     8761 cgtcctccta tcggcaaggc caggaaaccg tcgcatccgg ttactcctcg accatcccat
     8821 cgccagaaat ctgggagcgt tctcatggct gctgctggcg gtcggcctct cggccggaca
     8881 cccgaggatg gccgtgatcg tgggcggcac cgttttcatt gggcttctga tagggcttgt
     8941 caacggagca cggtggctgt tcggaaagag cgcacggcat ccgtagcaag ttctgcgaag
     9001 gcagtttgcg gtctttcaag gggaacgcga gagcaggacg gcataaatac agcggacggg
     9061 caatgcgaac gagatctgcg gacttctacg ggagcggttt gggcctctgg cccgcttcgg
     9121 gggccgctgt tcgccggctt ttgcagacgc caggcaatcg gcggacgatt ctatgtcgtc
     9181 caatgtcgtc cggtggaagc gctggccatg gatgccgcat tcggcggcag atttggccct
     9241 gaagacgtat tcctttccta taaagccgtc gcaagggcag actgacttgc gaggggcggg
     9301 ggtgacctgc tcaatcgctt ctgaatcagc gggccgtttc gaactcaaaa cagaagacca
     9361 gcaatttctc tcgctgaagt tggtctcaat cgcttctgaa tcagcgggcc gtttcgaacg
     9421 cggcgtcttt tgcctcggtc atcaatctca catctgtctc aatcgcttct gaatcagcgg
     9481 gccttcggcg ctcatgagaa ctgacccacc catgctccga tagagtgagc cagggggagt
     9541 gcaagttccg caacggagaa cggggctgga caacatcatc cacgccggtc ggcgtgctgg
     9601 gtttgttctg tcgtgtaggg gatcagcagc acgtcgaggc gcgccagccg gtggatcaac
     9661 ttgcgcgtgg agcgatcggc gagtgaagcg tacatctcgt cgaacagatc ctgcgcgcga
     9721 acaaactggc agcgcagccc gttctgcaaa gccttgagca ggattccgca ggcgagtccg
     9781 gttttcccca caccggtggg gccggtgaaa accagattct catgtttggc cacgaagtcg
     9841 agttcggcga aggcttggat ctgcttgcgc ttcacgccgg gttgccggct gtacgggaac
     9901 gtctccagcg accaacgctc ggggagattg gcgcggcgaa tgcgccactc cagcgcgctc
     9961 tcctggcggt gatgccactg ggagcggagc agcgcggcca cgaactccga gtaggagacc
    10021 tgctgtttcc cggcggcgcg cagttgctcg tcgtagatcg agcgcatgcg gccgagtttc
    10081 aggttcttca gcagttgttc gatctcgtca gtcatcggta tcctccagcc ggaagtattc
    10141 gcgcgtgacg cggcgcagga tcatgcgttc gacgcggtcc agatcgtaca agccatagcg
    10201 gccggcttct tcgatggcgc ccagaaaggc ttcgcgcgga tagtcgcgca gcatgcgcag
    10261 tagttgccgt aacagcagcg cggtcacctt gcggccgcgg catttcaggt tctcgatgta
    10321 aacagcgatc tcgggcgctt cggccagcag cgcggtttcc tccgggtgcg gatcgcgctt
    10381 acaaggcccc tggccccgcg gcggccggtg ctcgggcaac atcacgcgca cctgatcggg
    10441 cgtggcgata cgccggtgcc ggacgatgtg gcgggcgtcg agttcgatct cgacgtggtc
    10501 gcgcgtttcc ctcacttcca cctggcggcc gatgaacgat ggcggcacgg agtagcggtt
    10561 gctgtgcagc gacacatagc cttccacgtc caccagccgg tgatgcagcc ggtagacctc
    10621 cggcagccag gccggcagcg gtttcagcaa cgggcgctcg acggcgtaga gttcgcgcgg
    10681 cacggcacgg atatgtttct tgtaggtggc gttgacgcgg tcgcaccact ggcgcgcctg
    10741 gtggttcaga tcctgccaat cagcgaacgt gcgtccggcc agaaagttgt tctcgatgaa
    10801 gtggaacggc cgctcgacac gggccgaacg gttggcgtcg ccgatctcgt gcgccacgaa
    10861 acgaaagccg agacgctcgg cgaaggcagc catctccggc acgggttcca tcgcgcggcc
    10921 gctgccgcgc aacacgacca catgcgtgtt gtcgatcatg acgcgttcgg tcgcgccgtt
    10981 catataccgg accgcgtcgg tgagaaagac cttgcagtcg aaacgctgga acgcgggtag
    11041 cagttcgaag aacagcatgc gcgagtaaca tagcgttgcc gaggcggtct gcgctttgaa
    11101 cttgcgcccg cccacgatca cttcgtgggg cgatgtgtcg tgctgcgttt cctcgccggg
    11161 ggcgaactcg tagcggccgg ccggcacgac cggcgtctgc ccaattcctt cgcggcggca
    11221 gaaggcggtg agcgcctggt aggagaacgt cgcgccggtt gccgccaact cctcatggac
    11281 tcgcacgaga ttgcctttgc actgcggtag taactcgaga atctgctgac ggaatggcgt
    11341 cgctttctcg gcccgctcga tctggggcac ggcgctcgag ttcgcctgca acacctggcg
    11401 gacgctttgg cgcgagactt tcagtagccg ggcaatctcg cgtttcccca ctccctgccc
    11461 gcggagttcc agaatcgtgg ctcgttgtgc ttggctcagc atgattggct tcgggctcct
    11521 cgatgcgttc ctggagttcg gtcagcagtt cgatggcgcc ttggatcttc cgccgcacgg
    11581 cggcgcggtt cggcggcggt ggttccagat gctcccgcgc ccgttgcgcg atggccacga
    11641 tccgggtcgg ttcccggtcg agcgtctcgg cttgcgcttg cgtcttggcg aataatttcg
    11701 gcgccgccag gatgcgttcg cggacagcgc cgccggcatc tcgccaggcg cgatagaacg
    11761 cgcccgcctg ccgcgccgtc cacctctgtt cggcgaaagc ggcggccatg cgcaggcaat
    11821 gctcccggct gatgcgcgcc accggcgcca gataccgcgc ggcgagcgaa gcggcgacct
    11881 tgccttcccg taccagttgc tgcaccgatt cctgcagcgc ctccaccagc gccaggcgac
    11941 tggccaccca cgtccggccg cggtcgaagc gccgcgcgag ttcttccagc gagcagccca
    12001 agcggctttc catctccgcc agcagccagc cttgttcgat cgcgctctcc ggctcgctca
    12061 tccgcagcga ccggtccagc gccagcgcgt cggcttcgct catatcccag acgacggcat
    12121 cgaccgtgtc ccggcccaat tgctggagcg cggcgatgcg cttgtgaccg tcgatgacca
    12181 gatagcggcc gtcggtttgg atgacgatga tgggcgtttg ctggccggcc tcagccagtg
    12241 aagcgaccag ccggcgctgg cgtgccggat ggcgtacccg gaggcactcc aggcggcggt
    12301 ccagttggtg aaactccagt cgcatcggct tgggcttcag tatgcgccgg cgcggatctg
    12361 gtctt
//
LOCUS       DASTMK010000574         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_413099, whole genome shotgun sequence.
ACCESSION   DASTMK010000574 DASTMK010000000
VERSION     DASTMK010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413099"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFQ79_23515"
     CDS             <1..250
                     /locus_tag="VFQ79_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HEU0142711.1"
                     /translation="LAMQLPVIVERNAFTLPQERYNADWILEQDAGLVLRNFREIGGA
                     VRHMLQPEVLARMRANAKAIKNRAVFEIPGILKEILGR"
     gene            254..2227
                     /locus_tag="VFQ79_23520"
     CDS             254..2227
                     /locus_tag="VFQ79_23520"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0142712.1"
                     /translation="MRLELALLACLSAAFLHAQNPESAMLRWMDSEAQKLLAARDAEI
                     NAIRTVEEAERRKAAVRTKILDLIGGLPDYSGPLNARITGSIDHGQYRIEKVIFESLP
                     RYYVTGNLYVPNAPGKYPGVLMPMGHWDEGKTAGQRICANLALKGFVALAFDPVGQGE
                     RFQSYDPRLGASLAGGTTDQHFMNGPQSILAGENFARYRIWDAKRALDYLLSRPEVAA
                     DRIGCTGCSGGGTLTTYISALDDRIKVAAPSCYMNSFKLLFSGSIGDSEQSFPNFLSS
                     GLDQTDYVELFSPKPWLISSTEKDFFTPAGAKIVYEEAKRWYRLYNAEDRIKWVVGPG
                     GHGTPLEVREAVYDWFIRWLNDGKGDPREQQVTMHPNYELLATGAGQVSAEGSRELNE
                     IILERLEARRKTGSVEEMKAYLRKLAPASSGQPAVKSLGTRKESGLPVERISIQVEPG
                     LLIEGTLYTPASSGPKPGVLLVGNDPALATKIAAAGAVVLALNPRGTPKPDNSNRRLL
                     GDWLANTRAWLIGRNLPALRAADILKGVDILAARKDVREVRAAAREVSGVWLLMAAVL
                     DGRISRIWLDRTPADLISAVQAPIHRNLHDVAMPGFLLHWDVPDMVKAIGPDKILWTD
                     PANWMGGVIPLKGDYKYRYFEQPDEDFIQALLQ"
     gene            2262..3194
                     /locus_tag="VFQ79_23525"
     CDS             2262..3194
                     /locus_tag="VFQ79_23525"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU0142713.1"
                     /translation="MKLSLVYFVLAAATLAADFEIKDQAEFNRIFDATAKVERLATGM
                     KFLEGPAWFRSGGTEFLIFSDIPANELKKWTPAKGVETFRSPSNNANGNTVDREGRLL
                     TAEHGARRISRTEKNGTVVTLVDSFEGKALNSPNDVVVTSDGVIWFTDPDYGLAGRTK
                     EQPGNYVYRFDERTKSIRAVIKDFDKPNGLCFSPDETKLYVADSGKPRHIRVFNVKPD
                     GSVDTGSVFATIDKGGPDGIRCDKDGRIWSSSGDGAQIFAADGRLIARILLPEAAANL
                     AFGGPDGKTLYLTARTSLYAVGTRTAGAPRQRSR"
     gene            3252..3593
                     /locus_tag="VFQ79_23530"
     CDS             3252..3593
                     /locus_tag="VFQ79_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0142714.1"
                     /translation="MNTDKNRAGIPPGTLEMLILKTLKRFGPSHGYAIAKSIQQTSED
                     VLQVEEGSLYPALQRILMKGWVTAEWIPAGPKRRSRIYRLTPEGRKQLSAEISEYRRV
                     SQAIERVIEPT"
     gene            3606..>4207
                     /locus_tag="VFQ79_23535"
     CDS             3606..>4207
                     /locus_tag="VFQ79_23535"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEU0142715.1"
                     /translation="MKALSRIHRRLAVLFSRNRFDRDLEEEIQNHIEMQAEENQELGM
                     PTGEAYAAARRRLGNITLWKEISREVWGWAAIERLGQDLVYALRLLRRNPSFSTAAIL
                     TLALAIGLNTAIFSILNTVVLRPLPFQQPDRLVLIQEHFPKMDANPFGVSAPDCVKFQ
                     TQNRVFEDTAAFKDGEVDLSGTGEAERIRRGRVSAGLFRLL"
BASE COUNT          907 a         1216 c         1288 g          796 t
ORIGIN      
        1 gctcgccatg caacttcccg tgattgtgga gcggaatgct ttcacattgc cgcaggagcg
       61 atacaatgcc gattggattc tggaacagga tgccggcctg gttctgcgga attttcgcga
      121 aatcggcgga gcggtgcggc acatgctgca accggaggtc ctggccagga tgagggcaaa
      181 cgccaaagca atcaagaatc gggcggtatt tgagataccg gggatactta aagaaatact
      241 tggaaggtaa tcgatgagac ttgaacttgc cttactagcc tgcctgtccg cagcattcct
      301 tcatgcccag aatccggaat cggcaatgct ccggtggatg gattccgagg cacagaagtt
      361 gctggctgca cgcgatgctg aaattaacgc catccggacc gttgaagagg cggagcggcg
      421 aaaggccgct gtacggacca aaattctgga cctcattggg ggactccccg attactccgg
      481 gcctttgaac gcgcgcatca cggggagcat cgaccacggg cagtatcgca tcgagaaggt
      541 gatctttgaa agcctgccgc gatactatgt caccggaaat ctgtacgtgc ccaatgctcc
      601 cggcaagtat cccggtgtgc tgatgcccat ggggcattgg gacgaaggaa aaaccgcggg
      661 ccaacggatc tgcgcaaacc tcgcgttgaa aggctttgtc gcgctggcgt tcgatccggt
      721 cggacaggga gaacggtttc aatcctatga tccccgcctg ggagcatcgc tggcgggcgg
      781 aaccacggac cagcacttca tgaacggccc acagtcgatt ctggccggag agaacttcgc
      841 caggtaccgg atttgggacg ccaagcgcgc actggattat ttgctgtccc ggcctgaggt
      901 ggctgccgac cggatcgggt gtaccgggtg ctccggcggc ggcacgctga ccacgtacat
      961 ttccgcgctc gacgatcgaa tcaaagtcgc ggccccgtcc tgttacatga attccttcaa
     1021 gctgctgttt tccggttcca taggcgattc ggagcagagc ttcccgaatt ttctctcttc
     1081 ggggctcgac cagaccgatt acgtcgagct attttctccc aaaccctggc tcatcagctc
     1141 aaccgagaag gatttcttca cgcccgccgg cgcaaaaatc gtgtacgagg aggcgaaaag
     1201 atggtaccgc ctctacaacg ccgaggaccg catcaagtgg gtggtcggtc cgggagggca
     1261 cggcacgccg ctcgaagtac gggaggccgt ttacgattgg ttcatacgct ggttgaacga
     1321 cggcaaaggc gatccccgcg aacagcaggt cacgatgcat ccaaactacg aactgctggc
     1381 gacaggcgcg ggtcaggtgt cggcggaagg cagccgtgaa ctgaacgaga tcattctcga
     1441 acggctcgag gccaggcgga agaccggcag cgtggaggag atgaaggcct atctgcgtaa
     1501 gctggcgcct gcgtcttccg gacagccggc cgtgaaaagc ctcggcacgc gaaaggaaag
     1561 cggcctgccg gtggagcgga tctcgattca ggtggaaccc ggattgttga tcgaaggcac
     1621 actgtacaca cccgcgagca gcggaccgaa acccggtgtg ctgctggtgg ggaacgaccc
     1681 ggccctggca acgaaaatag ccgcggccgg cgcagtggta ctggcgttga acccgagggg
     1741 cacgccaaag cccgacaact ccaaccggcg gttgctcggc gactggctgg caaacacgag
     1801 ggcatggctg atcggccgca atctgccggc tttgcgcgcc gcggacatcc tgaaaggcgt
     1861 agacatcctg gccgcgcgca aggacgtccg tgaagtgcgg gcagccgccc gggaggttag
     1921 cggcgtctgg ctgctgatgg ccgctgtgct tgatggccgc atcagccgca tctggctcga
     1981 ccgcacaccg gctgacctta tcagcgccgt tcaggctccc attcatcgca acctgcacga
     2041 cgtggcgatg cccggcttcc tgctgcactg ggacgttcct gacatggtga aagcgatcgg
     2101 cccggacaaa atcctgtgga ccgaccccgc gaattggatg ggaggcgtga tccctctgaa
     2161 gggcgattac aagtatcgat acttcgagca gcctgacgag gatttcatcc aggcgttatt
     2221 acagtagcct gccgggcgtt gctgtgatac agttgcgctc gatgaaactg tctcttgtgt
     2281 atttcgtatt ggccgccgcc actctggcgg cggatttcga aatcaaggac caggccgaat
     2341 tcaacaggat cttcgatgca acggccaagg tggaacgcct tgcgaccggc atgaagttcc
     2401 tcgaaggtcc ggcctggttc cggagcggcg gcacggaatt cctgatcttc agcgatatcc
     2461 ccgcgaatga actgaaaaaa tggacgcccg cgaaaggcgt cgaaaccttc cgatctccca
     2521 gcaacaatgc caacggcaac accgtggacc gcgagggccg tctgcttacc gccgagcacg
     2581 gcgcgcggcg catatcgcgc accgagaaga acgggacggt tgtaaccctc gtcgattcct
     2641 tcgagggcaa agccctgaat tcgcccaatg atgtcgttgt cacatccgat ggcgtcatct
     2701 ggttcaccga cccggactac ggtctcgccg ggcgcacgaa agaacaaccg ggcaactacg
     2761 tttaccgctt cgatgagagg acgaagtcga tccgtgcggt catcaaagat ttcgacaagc
     2821 cgaacggact ttgtttctca cccgatgaaa cgaagctgta tgtcgccgat tccggcaagc
     2881 cgcggcatat ccgtgtgttc aacgtgaagc cggacgggtc cgtggacaca ggctctgtct
     2941 tcgcgaccat cgacaaaggc ggtccggacg gcatccggtg cgacaaggac ggccgcatct
     3001 ggagcagttc cggagatggc gcacagatct tcgccgcgga cgggcgcctg atcgcgcgca
     3061 tcctgctgcc ggaagcggct gccaacctcg cgttcggagg tccggacgga aagactttgt
     3121 acctgaccgc gcgaacgtcg ctatacgccg tcggcaccag gacggccggc gctccacggc
     3181 agcgttcccg ctgaaaatct gcatccttga tctacagtgg tgttctatgg aagtctatac
     3241 atagattatc tatgaacacc gacaaaaaca gagccgggat tccacccgga actcttgaaa
     3301 tgctgattct caaaacgctt aagcgattcg gcccgagcca cggctacgcg atcgcaaaat
     3361 ccattcagca gacctccgag gacgttctcc aggtggaaga aggatcgctt taccccgcgc
     3421 tacagcggat tctgatgaaa gggtgggtga cggcggagtg gataccggcc ggtcccaagc
     3481 gccggagccg catttatcgt ttgacaccgg aagggcggaa gcagctttcc gccgagatct
     3541 ccgaataccg gcgggtttca caggcaattg agcgggtcat cgagccgacc tgagagggca
     3601 aatccatgaa agctttgagc cggattcacc gcaggcttgc ggttcttttc agccgcaacc
     3661 gttttgatcg cgatctcgaa gaggagatcc aaaaccacat cgaaatgcag gccgaggaga
     3721 atcaggagct cggaatgccc accggcgagg cctatgcagc ggcccggcgg cggctgggga
     3781 acatcactct gtggaaagag atcagccgcg aggtctgggg ttgggcggcg attgaaagac
     3841 tggggcagga ccttgtttac gcgctgcgcc tccttcgcag aaatcccagc ttttcgacgg
     3901 cggcgattct gaccctggct ctcgccatcg gcttaaacac ggcgatcttc agcatcctga
     3961 acacagtggt tcttcgtccc ctgccgtttc agcagccgga ccgcctggtc cttattcagg
     4021 agcatttccc gaaaatggac gccaacccgt ttggtgtgag cgcgccggac tgcgtgaagt
     4081 ttcagacgca aaatcgcgtg ttcgaagaca ccgctgcctt taaggacggc gaagtcgatc
     4141 tttcgggaac cggtgaagcg gaacggataa ggcgcgggcg tgtctccgcc ggtctgtttc
     4201 gcctgct
//
LOCUS       DASTMK010000575         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_220878, whole genome shotgun sequence.
ACCESSION   DASTMK010000575 DASTMK010000000
VERSION     DASTMK010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220878"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..497
                     /locus_tag="VFQ79_23540"
     CDS             189..497
                     /locus_tag="VFQ79_23540"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEU0142716.1"
                     /translation="MADNENARVKELDLRPIPHGRRHGLVFETFDNLAVGESFIIVND
                     HDPSPLRMQMEFMRHGELSWDYLERGPEDFRVEIKRAALPASERPKASGSDPSVLTRF
                     "
     gene            complement(523..>4314)
                     /locus_tag="VFQ79_23545"
     CDS             complement(523..>4314)
                     /locus_tag="VFQ79_23545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEU0142717.1"
                     /translation="PLVTYEKTKQQFEVVCKIMNSPRVSRVVCATDAGREGELIFRYI
                     YEAAHCEKPFSRLWISSLTPDAIRKGFETLRPGQEYDALADAARGRSRADWLVGMNLS
                     RAYSLAYGEDLSVGRVQTPTLAMLVERELSIRSFVPEDYLEVVATFHPVGSPAENQYQ
                     GTWFRTKPDGEKDKEQQKATRLPATGEEAKQITERARSGQAAIESIESETQRMAPPLL
                     YDLTELQRHANRLFGFSAQHTLDLAQALYERHKLISYPRTDSRHLTQDVAATLTRVVG
                     VIETPYRESLAPGTGERPLGRRFVDDSKVGDHHAIIPTAFPPPSALPLEERKIYDLIC
                     RRLLSAWHEDHIWSVTTVITAIRNGDLCDRYHTSGTAVQQVGWKVLDLAPARKAEKGK
                     TKGEEEQAQQSLPPGLEKGQSQDVVDVETIKKKTRPPKRFTEATLLTAMETAGKTLDE
                     KELSDAMKETGLGTPATRAAIIEVLLKRGYIVRKGKSLEATDKGIRLVEVVHPEVRTP
                     AMTGQWEAFLARIQRGKAQLEPFLQGIEDYVRDVVGKTGTVQAPAKAAATAAAIGAPV
                     RDSVPEPADLPEIDTSGPLQSVLQRTFGFPHFRPNQEAVCQAAIEGKDVLLVMPTGSG
                     KSLCYQLPGIARGGTTLVISPLIALMEDQAAKLKQQGFAVERIHSGRERAVARQVCFD
                     YLNGKLHFLFIAPERLRVTGFPEMLAKRKPTLIAIDEAHCISQWGHDFRPDYRMLGQH
                     LPLLRPAPVIALTATATPLVQDDICKQLGLPRPAKFIHGFRRNNISIEAVEAAPSQRT
                     ALALQLLLESERRPGIVYAPTRKQVESVAAELGAHFPTAAYHAGLDAEHRQRVQQEFL
                     SGEVEVMVATIAFGMGIDKADIRTVIHTALPGSLEAYYQEIGRAGRDGNPSRAVLMHS
                     YADRYTHDFFFERDYPDVAVLDAMFARLRSEPIEKAVLQKQMRIDADLFDKALEKLWI
                     HGGAVMDYAENISAGEGHWRESYIAHGEQKRAQIDLMIRYAETNQCRMSALVRHFGDL
                     ADRQASCGICDFCAPENCAAQRFRAATEAERQTCFRVLAALHSGEQKSTGRLHAELFP
                     GAEMSRDTFEDVLGAMARAGLARLSDAVFEKDGKQIPYRTVRLTETGRAADETTQIDL
                     MMKSAAMPAEPRRRRKKGAAGAREPRTAAKKKPAARKRERPPAGEHSPIEEALRAWRL
                     GEARRRGVPAFRIFTDQTLRAIAQARPASAAELLAIPGIGINTVEKYGRQIYRILEES
                     R"
BASE COUNT          737 a         1384 c         1280 g          913 t
ORIGIN      
        1 aaatcgcgcc caaaaaggct ctgcgatcca tttggttctc gattgtatcg catgaacccg
       61 gtaaggcgtg tgtgacctcg gtcactggct taaacgctgg cagagtgcat gcttgaaagg
      121 agagatggcg aaacctggga acatcgtccc gccatcccgt tgaataacga actcagagga
      181 gaaatagcgt ggcagacaac gagaacgcgc gagtcaaaga gctggacttg cgcccgatac
      241 cgcacggaag aaggcatggc ctggtatttg agacttttga caatctggct gtcggcgagt
      301 cctttatcat cgtcaacgac cacgaccctt ctccgctgcg catgcagatg gaatttatgc
      361 gccatggaga attgagctgg gattacctgg agcgtggccc cgaggatttc cgtgtggaga
      421 tcaagcgggc tgcgcttcct gcatccgagc ggcctaaggc aagcggctcg gacccgtcag
      481 ttttgacgcg gttctgacaa ccggcggggc gccggcttcg ctttaccggg actcctccaa
      541 tatccggtaa atctgccgtc catatttttc gacggtgttg atccctattc ccgggatggc
      601 aaggagttcg gcggcactgg cggggcgcgc ttgggcaata gcgcgcaacg tctgatcggt
      661 gaagatccgg aacgccggca cgccgcgtct gcgcgcctcg cccagccgcc atgcacgcaa
      721 ggcctcctcg ataggggaat gctctcccgc gggagggcgc tcccttttcc gcgctgccgg
      781 cttcttcttt gccgcggtcc gcggctccct ggcgccggca gcgccttttt ttctccgcct
      841 tcgcggctct gccggcatcg cggcggattt catcatgagg tcgatctgtg tcgtttcgtc
      901 cgctgcccgt cccgtctccg tgagcctcac cgtgcggtat ggaatctgct tgccgtcttt
      961 ctcgaacacg gcatcggaaa gccgtgccaa gcccgcgcgc gccatcgctc ccaggacgtc
     1021 ttcgaaggtg tcacgactca tctccgcgcc cggaaaaagc tctgcgtgca gccgccccgt
     1081 cgatttctgc tcgccggagt gcagagcggc cagcacccgg aaacacgtct gccgctccgc
     1141 ctctgtcgct gcgcgaaagc gttgggccgc acaattttcc ggtgcacaga aatcacagat
     1201 cccacaggac gcttgccggt cggccaagtc gccaaagtga cggaccagcg ccgacatgcg
     1261 gcattgattg gtctcggcat accggatcat cagatcgatc tgagcccgct tttgctctcc
     1321 gtgcgcgatg taagattcgc gccagtggcc ctcgccggcg cttatgttct ccgcgtagtc
     1381 catcactgcg ccgccatgga tccacagttt ctccagcgct ttgtcgaaca gatccgcatc
     1441 gatccgcatc tgcttttgca gcacggcctt ttcgatgggc tcgctgcgca gccgtgcgaa
     1501 catcgcgtcc agcaccgcta catcggggta gtcgcgctcg aagaaaaagt catgcgtgta
     1561 gcggtctgcg taggagtgca tgaggacggc gcggctcgga ttgccgtcgc gtccggctcg
     1621 cccgatctcc tggtagtatg cttccagact gcccggcagg gcagtgtgga tcaccgtgcg
     1681 tatatcggct ttgtcgattc ccatcccgaa cgcaatggtc gccaccatca cttcaacctc
     1741 tccggaaaga aactcctgct gcacccgctg gcgatgctcg gcgtccagtc ctgcgtgata
     1801 ggccgccgtg ggaaaatgag cgcccaattc ggccgccacg ctttccacct gtttccgggt
     1861 aggagcatac acgatacccg gcctgcgttc gctctctaac aatagttgga gcgctaaggc
     1921 ggttctctgt gacggcgccg cttccaccgc ttcgatcgaa atgttatttc gccggaaacc
     1981 gtggatgaac ttcgccggcc gcggcaggcc caattgtttg caaatgtcgt cctgcaccag
     2041 cggcgttgcc gtagccgtaa gcgcgatgac gggcgccgga cgcaataggg gcaggtgctg
     2101 ccccagcatt cgataatcgg gccggaagtc gtgcccccac tgggatatgc aatgtgcttc
     2161 gtcgatggcg atgagggtgg gctttcgctt cgccagcatc tccgggaacc ctgtcaccct
     2221 cagccgctcc ggcgcaatga agagaaaatg caacttcccg ttcaaataat cgaagcacac
     2281 ctggcgcgcc accgcccgtt cgcggccgga atgaatacgc tccacagcga agccctgttg
     2341 tttgagttta gcggcctggt cctccatcaa tgcgatgagg gggctgatga ccagcgttgt
     2401 cccgccccgc gcgatccccg gcaactggta gcatagcgac ttgcccgaac cggtcggcat
     2461 caccagtagc acgtctttgc cttcgatagc cgcttggcac accgcttcct gattcggacg
     2521 gaaatgggga aacccaaaag tgcgttgcag gacactctgg agcgggccgc tggtgtcgat
     2581 ctcgggcaag tccgcgggct caggcacgct atcgcgaacc ggcgcgccaa tcgccgcggc
     2641 ggttgcggcc gcttttgcgg gcgcttgtac cgtaccggtc tttccgacca cgtcccgcac
     2701 gtaatcctct atgccttgaa ggaacggctc gagctgtgct ttcccgcgct ggatgcgtgc
     2761 cagaaaagcc tcccactgcc ccgtcatggc cggggtccta acttccgggt gcaccacctc
     2821 caccaggcgg atccccttgt cggttgcctc cagactcttg cctttgcgca cgatatagcc
     2881 gcgcttcaga agcacctcga tgatcgcggc gcgggtggcg ggcgtcccca ggccggtctc
     2941 cttcatcgcg tccgaaagtt ctttctcgtc gagggtcttt cccgctgttt ccatcgcggt
     3001 cagcagcgtg gcctcggtga aacgcttcgg cggccgggtc ttcttcttga tcgtctcgac
     3061 atccacgaca tcctgcgatt ggcccttctc cagacccggc ggcaaactct gctgggcctg
     3121 ttcttcctcg ccctttgtct tgcccttctc cgctttcctg gccggcgcca gatccaggac
     3181 cttccagccc acctgctgca ccgccgtgcc cgatgtgtga taccggtcgc agaggtctcc
     3241 attgcgaatc gccgtgatga ccgtggtgac ggaccagatg tggtcttcgt gccaggcgct
     3301 gagcagccgc cggcaaatca ggtcgtagat cttccgttcc tcaagcggca gcgcggacgg
     3361 gggcgggaaa gccgtgggga tgatcgcgtg atgatcgccg accttggagt catcgacgaa
     3421 ccggcgtccc agtggacgct cgccggtgcc cggagcaaga gattcgcgat acggcgtttc
     3481 gattacgcca actacgcgcg tcaacgtcgc cgctacatcc tgcgtcaagt gccggctgtc
     3541 ggtgcgcggg tagctgatca gtttatgccg ttcgtagaga gcctgtgcca ggtcgagcgt
     3601 gtgttgtgcg ctgaagccga acaggcggtt cgcatgccgt tgcagctcgg tgaggtcgta
     3661 tagcagcggg ggagccatgc gctgcgtttc cgattcgata gactcgatgg ccgcctgccc
     3721 gctccgggca cgttcggtaa tctgcttggc ctcttctccg gtcgcaggca ggcgcgttgc
     3781 cttctgctgt tctttgtctt tttcgccatc gggctttgtt cggaaccagg tgccctggta
     3841 ttggttttcc gccggcgacc ccaccgggtg aaacgtcgct accacttcca ggtagtcttc
     3901 cggaacaaag ctgcgaatcg acagctcgcg ctccaccagc atcgccagtg tcggcgtctg
     3961 tacgcgcccg accgacagat cctccccgta ggcaagcgag tacgcgcgcg acaggttcat
     4021 gcccaccagc cagtcggccc ggctgcggcc gcgtgctgcg tcggccagtg catcgtactc
     4081 ctgacccggc cgcagagtct cgaagccttt gcggatcgcg tccggggtca gcgacgagat
     4141 ccacaaccgg ctgaacggtt tctcgcaatg cgcggcttca tagatgtagc gaaagatcag
     4201 ctcgccttcg cggcccgcat cggtggcgca caccacgcgc gagacgcgag gcgaattcat
     4261 gatcttgcac accacctcga attgttgttt ggttttctcg taagtaacca gcgg
//
LOCUS       DASTMK010000576         9034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_572524, whole genome shotgun sequence.
ACCESSION   DASTMK010000576 DASTMK010000000
VERSION     DASTMK010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572524"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFQ79_23550"
     CDS             <1..447
                     /locus_tag="VFQ79_23550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142718.1"
                     /translation="DGMRAAAAEGLGRLKNSADLPAIEKAFNEERKNNARLSLAFAMV
                     MLGKAETTEFSPLQYLINTLNSTAYRGVARPFLVELSRDTGIRQALYPAARTGTKDEK
                     IEIAQILARSGQKDSLPVLENLSRDTDPEVAQEGLRALRSLKARLP"
     gene            complement(450..659)
                     /locus_tag="VFQ79_23555"
     CDS             complement(450..659)
                     /locus_tag="VFQ79_23555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142719.1"
                     /translation="MAIPNVKRFRPFCPTCNDTFTLMAVLPKDASVEAYLADAIERHD
                     HKAFQESKNQHFKIRVGQQKQKRSK"
     gene            complement(691..1308)
                     /locus_tag="VFQ79_23560"
     CDS             complement(691..1308)
                     /locus_tag="VFQ79_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142720.1"
                     /translation="MKKVFLSILVAATAAFAQYTSEAAGAPPAEASALAGVLQSEGVK
                     ISGSNGPAMEFWFVKEMPGGATAPEENATLTEVPHGAFLGVVSFPAAGQDRRGQQIKP
                     GVYTIRYSLFPIDGAHQGVEPQRDFFVLSPAATDQDPAAKPDYETLMKMSLKASGTPH
                     PLVFSFWKEDTGAKPGLTQEGETWVLRTNIGSTLVGIIVVGVHAG"
     gene            1451..2125
                     /locus_tag="VFQ79_23565"
     CDS             1451..2125
                     /locus_tag="VFQ79_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0142721.1"
                     /translation="MQTILVIDDDESLRDTIGVMLEQEGFRVLLAGDGRSGLEKALTI
                     KPDLAIVDLRLPGLNGTEICKQLRAAQNKTPIIVLSAIGDEVDKVLLLEIGADDYVVK
                     PFGRRELLARIRAILRRVSPGTHTLLHVGETEVDLQRRSVIRNGEEVRMTPAEYNLLI
                     YFLQNPDRPLTRDMILNSVWGYDSFPNTRTVDAHVVKLRQKLEPDPNVPRHFITVHGV
                     GYRFLP"
     gene            2201..2596
                     /locus_tag="VFQ79_23570"
     CDS             2201..2596
                     /locus_tag="VFQ79_23570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0142722.1"
                     /translation="MPRLILLVEDSEQCASNLEIALSTLPGVEVIWAPSGREALHVLD
                     TTVERRISAVVTDLDMPDVDGFELVKAVRTHSRYAGLPIIVVSGNTDPELGRRLQALG
                     ADAFLSKPYSPGEVRNKLEQYLYGRLKRD"
     gene            2658..4022
                     /locus_tag="VFQ79_23575"
     CDS             2658..4022
                     /locus_tag="VFQ79_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142723.1"
                     /translation="MREWLSGYPVFLSVLIGTAMGFIPCVAQSSPDLANILRRIENLE
                     RENQALRKEVGELRAIVGAGATASAPETGIGRIEERVQIQERRTEELAQTKVETSQKL
                     PIRLTGMALFNLYHSGPRTGASENPVFAAQTEGRRVGGGTFRQSVIGLEYRGASTVWG
                     GRVHGSLFLDFYNGGERDFFTYARIRTASIELEWKTRSILAGLEKPIFAPREPNSLSQ
                     VGISPLTGAGNLWRWQPQVRFAQRVPLSGATELRAQFGVLETSESYPGIQLPAGAQVE
                     RRRPGAEGRFELAHALDDTRRIEFAPGFHFSTTHVGGASVPSSLFSLDWFANPWSRLE
                     FSGAFFSGKNVAHFGATRQGFTFLSDGRVIPVHSHGGWGQFTFIATPRLSFNVYGGQH
                     DDRDADLSPGGISRNMAGAANLMYRFAPNVIVSFEAAQLRTTYLGSGIRLNNRYDLAL
                     AYLF"
     gene            3994..4707
                     /locus_tag="VFQ79_23580"
     CDS             3994..4707
                     /locus_tag="VFQ79_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142724.1"
                     /translation="MIWLWLICFSLLKAGESAAATVTGHVTLADSREPSVRERKDYSG
                     VVVWLEAVGSGERLPAPGKFVMEQKGKRFLPHVLVVPVGATVDFPNFDPIFHNAFSNF
                     SGQPFDTGLYAPGTSQSVHFRRPGIVRVFCNIHSTMSAVIVVLPTHLYAVTSSDGTFR
                     LTDVPPGTYQLRFWHERSPEETLKKMERTLRVESANEKLPLVTISESGYLQVPHKNKY
                     GKEYAPPPREHSVYPGARK"
     gene            4704..6506
                     /locus_tag="VFQ79_23585"
     CDS             4704..6506
                     /locus_tag="VFQ79_23585"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0142725.1"
                     /translation="MRLPRMSLLVKIWVWTSVVLTAVSGIAGWLLQRQALETTFRSVE
                     EEVKASFQAYESLWRMRAESLRSVSAIISSMPHVRAAFGTRDHATIRDTTSEIWTRVS
                     DDLQETSFFLVTDPEGNTIASLDVQTTQAPRLYWPVVRSVRSRFPDQVSGFSIRDGEL
                     FQIVLTPVYVDSTSGPALINVLLTGYSVNHLLAQRFKEATGGSDFVFLSDGKIFASTL
                     NDRATGVLAARITAGESAVTLSDGVSQYVPLVRNLIDLEGNPVGKLAIFRSFDSAREQ
                     VAHLRRTLVLLWLAALAAGLGLMYLVARRIVQPVKELDRAAAEVARQNYEYRVQPSSQ
                     DELGRLASTFNTMCGSLQSARAELIRQERISTIGRMASSIVHDLRNPLAAIYGGAEMM
                     VDTDLTRDQMSRVAGNIYRASRRIQELLQDLVNVSHGRGGEKEVCRLAEIVEAAIETS
                     RAPAESRSVRLLANVAEEIEVNAARARLERVFLNLIDNAIEAMPSGGEVHIRAHTEGG
                     YVLVEVEDTGPGLSEEIRSQLFQPFTTRGKKNGLGLGLALSRQTVLDHGGDIWAASEQ
                     GRGAKFCLRFPLAESNDTPPGGLHAATFKTPPIT"
     gene            complement(6630..7586)
                     /locus_tag="VFQ79_23590"
     CDS             complement(6630..7586)
                     /locus_tag="VFQ79_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142726.1"
                     /translation="MCKLTSILLFFTMSTFAAVKIGKTSYAGWPNCYRISNGEVELIV
                     TSDIGPRIMRYGFAGGQNMFKEFADMLGKSGESSWQARGGHRLWVGPESMPMTYALDN
                     GPVKIDVRGDVLEAIQPVEKETGLQKSMVIKLAGSGTRVEVLHRIRNTTLFPVEFAPW
                     ALTMMAPGGLGITGFPPRGKHPEMLAPTNPLVMWAYTDLSDKRWRFSSKYMGLQQDPN
                     NASPQKLGHFNANTWGAYLLGSDLYIKRSKADESRTYPDFGCSFETFTNNEFLELETL
                     GPMTKVEPNGTVEHTEHWSLHRNVSIPHWDDATLDAVLLPLL"
     gene            complement(7596..8510)
                     /gene="thiL"
                     /locus_tag="VFQ79_23595"
     CDS             complement(7596..8510)
                     /gene="thiL"
                     /locus_tag="VFQ79_23595"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU0142727.1"
                     /translation="MTEIHIIERLRRKLPVFPRSGIVLSVGDDCAIFRPKGSPEDLLF
                     TTDLLIEDIHFHRTTHTPEDVGHKAVARGLSDIAAMGGEPRFCLLSVALSTGLEGAWM
                     DRFYRGALRLAGIHKLTIAGGDLAHAARFACDVVVCGAVPRGEALTRSGARPGDSIYV
                     SGVLGGSALGLESRSAAARKRHLRPEPRVALGCLLRRKVRTSAAMDLSDGISLDLHRF
                     SVASGVSAVIDHPLPSFPGASVEQVLHGGEDYELLFTVAPGTRVPRHHDGVPLTRIGS
                     IVERRRMRVAFFGRELEPLGYDHFRLAT"
     gene            complement(8531..>9034)
                     /locus_tag="VFQ79_23600"
     CDS             complement(8531..>9034)
                     /locus_tag="VFQ79_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142728.1"
                     /translation="WVSPSGTSLIAVLDMSGRVTPLVQSSESALFSPSISHDGKWMTL
                     IVRKPPDDHRVAIIPLRDGSAASQTEWVFVTEPGAWVNKPRWSPEADALYYVSDRDGF
                     VCVWMCRLDLKTKKPVEQPRVIAHFHDARASLGGVFGLELAVAKNKLVFNLGESSGNI
                     WLAPSGN"
BASE COUNT         1807 a         2724 c         2688 g         1815 t
ORIGIN      
        1 gatggaatgc gcgccgcggc cgccgaaggc ctggggcggc taaagaactc cgctgatttg
       61 ccggcaattg agaaggcgtt caacgaggag cgcaagaata acgcgcgtct ttcgctcgcg
      121 ttcgcaatgg tgatgttggg aaaggccgag acgacggagt tcagcccgct gcaatacctg
      181 atcaacacct tgaactcgac agcctacaga ggcgtggctc gtcccttcct ggtggagtta
      241 tcacgcgaca caggtatccg gcaggctctc tatccggcgg cccgcactgg aacgaaagat
      301 gaaaaaatcg aaatcgcaca gatcctggcg cggagcggac aaaaggactc gcttcccgtg
      361 ctggagaatc tgtcgaggga taccgacccg gaggtcgctc aggaaggctt gcgggcgctt
      421 cggagtttga aggcccgcct gccgtaatct tatttcgatc tcttctgctt ctgctgaccg
      481 acgcggattt tgaagtgctg gttcttggat tcctggaacg ccttatggtc atgccgttca
      541 atggcatcgg ccaggtaggc ctccacgctg gcatcctttg gcagcaccgc catcagcgtg
      601 aaggtatcgt tgcaggtcgg gcagaagggc cgaaaacgct tcacatttgg aattgccata
      661 cttccttcca gtttaacacg cagccggcga ttaaccggcg tggacaccca ccacaataat
      721 ccccacgaga gtgctcccga tattcgttcg cagcacccag gtctcgccct cctgcgttaa
      781 ccccggcttt gcccccgtgt cctctttcca gaaactgaac accagcggat gaggagtgcc
      841 cgaagccttc aggctcatct tcatcagcgt ttcgtaatcc ggtttcgcgg cagggtcctg
      901 gtctgtcgcg gcaggggaaa ggacaaaaaa gtcccgctgc ggctcaactc cctgatgtgc
      961 tccatcaatc gggaacaggc tgtagcgaat agtgtagaca cccggcttga tctgctggcc
     1021 gcgccggtcc tgtcccgccg ccggaaaact tacaacacca aggaaagctc cgtgcggaac
     1081 ttccgtcaag gtcgcgttct cttcgggtgc agtggcgccg cccggcatct cttttacaaa
     1141 ccagaactcc atcgccggac cgttggaacc tgaaattttc acgccctcgc tctgaagcac
     1201 ccctgcgaga gcagaggcct ctgccggagg cgcaccggca gcctcactcg tgtattgcgc
     1261 aaaagctgcc gtcgctgcca ccagaatgga aaggaagact ttcttcatca atatcgcgct
     1321 ccttattctc atcgtacaga ctattggatg tgcggtggcg gtcacggttt ccgccgatcc
     1381 tgtacaatca cttaacaaag acaagcataa aaggattaca cgagctgagg gagcggcctc
     1441 gggcattctt atgcaaacga tactcgtgat cgatgatgac gagagcctcc gggataccat
     1501 cggcgtcatg ctggagcagg agggcttccg ggtccttctg gctggcgacg gccgctcggg
     1561 actcgagaag gcccttacga tcaaacccga tctggccatt gtcgacctcc ggttgcccgg
     1621 gttgaacggg acggagattt gcaagcagtt gcgggccgcc cagaacaaga ccccgatcat
     1681 cgtactcagc gccatcggtg acgaagtcga taaggtgctg ctgctcgaga ttggcgccga
     1741 cgattatgtc gtaaagccgt tcgggcgccg tgagctgctt gcccggatcc gcgcgattct
     1801 ccgccgtgtt tctccgggta cccacacgct gctgcatgtt ggcgaaacgg aagtggatct
     1861 tcagcgccgc tccgttatcc ggaacggcga ggaagtgcgg atgacgcccg ccgagtacaa
     1921 cctgctgatc tatttcctcc aaaaccccga ccggcctctc acccgcgata tgattctaaa
     1981 ttccgtctgg ggctacgatt cctttccaaa tacgaggacg gtggatgcgc atgtggtcaa
     2041 actccgccag aaacttgaac ccgatccgaa tgtgccgcgc cattttatta cggtccatgg
     2101 agtgggatat cgatttctgc catgaagggg tttaacaacg agttaacaag tcggatggcc
     2161 cgccgctcga aactgtcggg gagaatcggg gaagatgcag atgccgcggc tgattttgct
     2221 ggtggaagat tccgagcagt gcgcttcaaa cctcgagatc gcacttagta ctctgccggg
     2281 tgttgaagtc atctgggctc cgagcgggcg cgaagccctc catgtgctgg atacaaccgt
     2341 cgagcggcgg atttccgccg tcgtcaccga tctcgatatg ccggatgtag atggtttcga
     2401 actcgtcaaa gccgtacgca cacactcgcg atatgccggt ctcccaatca tcgtcgtaag
     2461 cggaaacacg gatccggaat tgggcaggcg gctacaggct ctgggcgctg acgcttttct
     2521 ctcgaaacct tactcgccgg gcgaggtccg aaacaagttg gaacagtacc tttatgggag
     2581 gctgaaacgt gattaaacgt ggcgtcacat cccctccctc gcgagtgtgg ctcagcattc
     2641 gtttccgagc cacgaccgtg agggagtggt tatcgggata ccccgtcttc ctctccgttc
     2701 tgatcgggac tgccatgggg tttatcccgt gcgtggctca atcgtctccg gacctggcga
     2761 acatcctgcg gcgaatcgag aacctggaac gggagaacca ggccttgcgc aaagaggtcg
     2821 gcgagcttcg cgcaatcgtt ggtgcaggcg ccacggcgtc cgcccccgaa accggcatcg
     2881 gccgcatcga ggaacgcgtt cagattcagg agcgccgcac agaagagttg gcccaaacca
     2941 aagtggagac ttcacagaag ctgccgatac gcctcaccgg tatggcgctg ttcaacctct
     3001 atcacagcgg gcctcgcacc ggtgcttcgg aaaatcctgt ctttgctgca cagaccgagg
     3061 gccggcgagt gggaggcggc acgttccgcc agtccgtcat cgggttggag taccggggcg
     3121 cttccaccgt ttggggcggc agggtccatg gctcgctctt tctcgatttc tataacggcg
     3181 gcgagcggga cttctttacc tatgcccgca tccggactgc ttccatcgaa ctggagtgga
     3241 agacacgcag cattctggcc ggtctcgaga aacccatctt cgccccccgc gagccaaact
     3301 cgctatcgca ggtcggcatc tctccgctca cgggcgccgg caacctgtgg agatggcagc
     3361 cgcaagtccg cttcgcacaa cgcgtgccgc tgtccggagc gacggaactg cgcgcgcaat
     3421 tcggcgtgct cgagacttcg gaaagttatc cgggaatcca gttgcctgcc ggtgcgcagg
     3481 ttgagaggcg ccgcccgggg gcggagggcc gcttcgaact ggcacatgcg ctcgacgata
     3541 cacgtcgaat cgaattcgct cccggatttc acttcagcac gacgcacgta ggcggggcat
     3601 cggtgccctc cagcctgttc tcgctcgact ggtttgcaaa cccatggtcc cgcctcgaat
     3661 tttcaggcgc gtttttctcg ggcaagaacg ttgcgcattt cggggcgacc cggcaaggat
     3721 tcacctttct gtccgacggg cgggtgattc ccgtgcacag ccatggaggg tggggccagt
     3781 tcaccttcat cgcgactcca cgcctgtcct tcaacgtcta tggagggcag catgacgacc
     3841 gcgatgccga tctttcaccg ggaggcatct ccaggaacat ggcgggcgcg gcgaacctca
     3901 tgtatcgttt cgcgccgaac gtgatcgtca gcttcgaagc ggcacagttg cgcacgacgt
     3961 atctcggcag tggaatccgt ttgaacaacc ggtatgatct ggctctggct tatctgtttt
     4021 agcctgctca aagcgggcga atcggcagcc gcgacagtca ccggccatgt gactctggcc
     4081 gattcacgcg agccgagcgt tcgcgagcgg aaagactact caggcgtcgt cgtgtggctt
     4141 gaagctgttg ggagcgggga gcggttgccg gctccgggaa aattcgtgat ggagcaaaag
     4201 gggaagagat tcctgccgca cgtgctcgta gtgccggttg gcgcaaccgt cgattttccc
     4261 aacttcgatc cgatctttca caatgcgttc tccaacttta gcggccagcc gttcgatacc
     4321 ggcctctatg ctcccggcac cagccagagc gttcatttca ggcgcccggg catcgtaagg
     4381 gtgttctgca acatacactc cacgatgagc gcagtcattg tggtgttgcc gacgcacttg
     4441 tacgccgtca cctcttccga cggtactttt cgtttgaccg atgtgcctcc cggcacttac
     4501 cagcttcgtt tctggcatga gcgttcgccc gaggagaccc ttaagaagat ggagcgcacg
     4561 ctgcgcgtcg aatcggcgaa cgagaaacta ccgctcgtga cgatatcgga gtccggctat
     4621 ctccaggtgc cgcacaaaaa caaatatggc aaggagtatg ctccaccgcc gcgcgagcat
     4681 tccgtttacc cgggagcgcg caaatgagac tgccgcggat gtcgctgctc gtgaaaatct
     4741 gggtctggac gtcggttgtg ctcaccgcgg tgtccggcat tgcaggctgg ctgttgcagc
     4801 gccaggcgct ggaaaccacc ttccgcagcg tcgaggaaga agtgaaggca agctttcagg
     4861 catacgagtc tttgtggcga atgcgcgccg aaagcctgcg ttcggtgtcg gcaatcatca
     4921 gcagcatgcc gcacgtacgg gcggcgttcg gcacgcgcga ccacgccacc atccgcgata
     4981 ccaccagcga gatatggacg cgagtatcgg acgacctgca ggagacttcg tttttcctcg
     5041 tgaccgatcc ggaaggcaac accattgctt cgctcgatgt gcaaaccaca caggctcccc
     5101 ggctttattg gcccgtggtg cgctccgtgc gcagccgctt ccccgaccag gtttcgggat
     5161 tctctattcg cgacggcgag ctctttcaga tcgtgctcac accggtgtac gtggactcca
     5221 cctcgggacc tgccctcatc aatgttctgc tcaccggcta cagcgtgaat cacctgcttg
     5281 cacagcgctt taaggaggcc accggcggca gcgatttcgt atttctgtcg gacggtaaga
     5341 tcttcgcatc cactctcaat gaccgtgcaa cgggagtgct ggcggcgcgc atcaccgccg
     5401 gtgaatccgc agtcacgctc agtgacggag tatcgcaata cgtcccactg gtccgcaatc
     5461 tgatcgacct cgaagggaat ccggtaggca agctggccat ctttcgttcc ttcgacagcg
     5521 cccgcgaaca agtggcccat ctcaggcgca cgctggtgct gctatggctt gcggccctgg
     5581 cggcgggcct gggactgatg tatctggtgg cgcggcgcat tgtgcagccc gtgaaggaac
     5641 tggatcgcgc cgccgccgag gtggcccggc agaattacga gtaccgcgta caaccctcca
     5701 gccaggacga gctcgggcgt ctcgcctcga cgttcaacac gatgtgcggt tcgcttcaat
     5761 cggctcgcgc cgaattgatc cggcaggagc gcatctccac gatcggaagg atggccagct
     5821 ccattgttca cgacttgcgg aatcccctgg ctgccattta cggcggagcc gaaatgatgg
     5881 tggataccga tctcacacgc gatcagatga gtcgtgtcgc gggcaatatc tatcgggcgt
     5941 cgaggcggat acaggagtta ctccaggacc tggtgaacgt gtcgcatggc cgcggcggcg
     6001 agaaggaggt ctgccggctg gccgaaattg tggaagcggc gatcgaaacc agccgcgctc
     6061 cggcggagtc gagatccgtg cgattgttgg cgaatgtggc cgaagagatc gaggtcaacg
     6121 ccgcccgcgc gcgattggag cgtgtgttcc tgaacctgat cgacaacgcc atcgaggcga
     6181 tgccgtccgg aggcgaggtt cacattcgcg cccacactga gggtggatac gttctggtgg
     6241 aagtggagga caccggcccc ggactatcgg aagagatccg cagccagctt ttccaaccct
     6301 tcacgacacg cggcaagaaa aatggtttgg ggctcggcct tgcgctctcc cggcagacgg
     6361 ttctcgacca cggaggcgac atctgggccg cttcagaaca aggccgcggc gcaaaattct
     6421 gtctgcgatt cccgcttgcc gagtcgaacg atacgccgcc gggtggatta cacgccgcca
     6481 ccttcaagac gccgccgatt acttaataca cggtttcgga agttcacttt tgcgacgggg
     6541 ccgctccctc acggtcacgg ctcagaaacg gagccgcgcg cgtgagcaag cggtatcgga
     6601 taggtcaccg taattccgaa tagaagtact taaagaagag gcagcaggac ggcatccagc
     6661 gtcgcgtcgt cccagtgcgg aatcgagaca ttccggtgca gagaccaatg ctccgtatgt
     6721 tccacggttc cgttcggctc taccttggtc atgggcccga gtgtttccag ttccaggaat
     6781 tcgttattcg tgaaggtctc gaaggagcag ccgaagtccg ggtaggtcct gctttcatcc
     6841 gccttcgatc gcttgatgta caggtctgag cctagcaggt atgctcccca cgtgttcgcg
     6901 ttgaaatgcc cgagcttctg cggcgacgca ttgttcgggt cctgttgcag gcccatgtac
     6961 ttgctgctga atcgccatcg tttatcggac aggtccgtat acgcccacat caccagcggg
     7021 ttcgtgggag cgagcatttc cgggtgtttg ccgcggggcg gaaagcccgt aatgcccagg
     7081 ccgcccggcg ccatcatcgt cagtgcccag ggagcgaact cgacaggaaa cagcgtggtg
     7141 ttccggatcc ggtggagaac ttcaacccgg gttccggaac cggcgagttt aatcaccatg
     7201 cttttctgaa ggcccgtctc tttttccacc ggctgaatcg cctccagaac atcgccgcgc
     7261 acgtcaatct tcaccggtcc attgtcgagg gcatacgtca tcggcatcga ttcggggccc
     7321 acccacaatc ggtggccgcc acgcgcctgc cagctcgatt cgccgctctt gccgagcatg
     7381 tccgcaaatt ccttgaacat gttttgcccg ccggcgaatc cgtagcgcat gatgcgcggg
     7441 ccgatgtcgc tggttacgat cagctcaacc tctccattgg agatccggta gcagttgggc
     7501 catccggcat agctggtttt cccgatcttt acagcggcga aggtactcat ggtgaaaaag
     7561 agaagaatgc tggttagctt gcacatcgtt gcttattatg tcgcaagccg gaagtgatcg
     7621 tagccgagcg gctccaactc ccgcccgaag aatgcgaccc gcatccgtcg gcgttcgaca
     7681 atggacccga tccgcgtgag gggaaccccg tcgtggtggc gcggtactcg cgttcccggt
     7741 gcaacggtga agagcagttc gtagtcctcg cctccgtgta gcacttgctc aacgcttgcg
     7801 cccggaaacg atggcagcgg gtgatcgatt accgccgata caccggaggc aaccgagaac
     7861 cggtgcaggt cgagcgatat cccatcgcta agatccatcg cggcgctggt gcgaacctta
     7921 cgccgtagaa gacacccgag tgcaacacgc ggttcggggc ggaggtgcct cttgcgcgcc
     7981 gcagcggaac gcgattcgag acccagggcc gagccgccca aaacgccgga cacataaatc
     8041 gaatcgcccg gccgcgcgcc ggatctcgtc agagcttctc cgcgtggaac cgcgccgcac
     8101 acgaccacgt cgcaggcgaa ccgggcggca tgcgccaggt ctccgccggc gatcgtaagc
     8161 ttatgaatgc cggccaaccg cagcgcacct cgatagaacc ggtccatcca ggcgccctcc
     8221 agtcccgtag aaagcgcgac cgaaagcaga cagaagcgcg gctccccacc catggccgcg
     8281 atgtcgctca agcctctcgc aacggctttg tgtcctacat cctcgggcgt atgcgtcgtg
     8341 cggtgaaagt ggatgtcctc aatgagcagg tctgtggtga acagcaggtc ttcgggtgaa
     8401 cccttcgggc ggaaaatggc gcaatcgtct cccacactca gcacgatgcc ggaacgcggg
     8461 aagaccggca atttccgccg cagccgttcg ataatatgga tttccgtcat ttccaattgg
     8521 gccgggccgc tcagttgccc gagggggcga gccagatgtt tcccgaactt tctccaagat
     8581 tgaagacgag cttgtttttc gccaccgcca attcgaggcc gaacacgcct ccaagagaag
     8641 cgcgcgcgtc gtgaaagtgc gcgattaccc tgggttgctc gaccggcttc ttcgtcttca
     8701 aatcgagtcg gcacatccag acacaaacga acccgtcccg gtccgagaca tagtagaggg
     8761 catcggcttc cggcgaccag cgtggcttat tgacccaggc gccaggttcg gtgacgaaga
     8821 cccactcagt ctgcgaagcg gcagatccat cccgaagcgg aataatggcg acgcggtggt
     8881 cgtcgggagg cttccggaca atcaaggtca tccatttacc atcgtgcgag atggacggtg
     8941 aaaacagcgc gctctcggag gattgcacca gcggcgtgac gcggcccgac atgtccagaa
     9001 cggcaatgag cgaggttccg gatggggaga ccca
//
LOCUS       DASTMK010000577         7203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_62605, whole genome shotgun sequence.
ACCESSION   DASTMK010000577 DASTMK010000000
VERSION     DASTMK010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62605"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFQ79_23605"
     CDS             <1..883
                     /locus_tag="VFQ79_23605"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0142729.1"
                     /translation="GTYDPQLGLLYWPTGNPCPDFNGDERKGDNLYSDSVVALSVKTG
                     QLKWYYQFTPHDLWDWDAQQPPVLVDVEWKGRPRKLLLHANRNGFLYVLDRISGELLL
                     GEPFVKKLTWASGIGKDGRPMKIPGQSPSPEGTKVCPAVEGATNWFSTSFHPDTGLYY
                     VQTLEKCNIFVKEVGKWEPGKSYYDGSTRQVPGEPGQKILRAIDIRTGKIAWELPQAG
                     DANTWGGTLATVSGLVFFGDDSNNFSAADATTGRLLWSFPANHLWKASPMTYIFDHKQ
                     YVAVASGPNVIAFALVE"
     gene            1022..2068
                     /locus_tag="VFQ79_23610"
     CDS             1022..2068
                     /locus_tag="VFQ79_23610"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142730.1"
                     /translation="MLGTKLFRACEAALLIIGLLALSAPATLEAQTTGTISGFVTDPS
                     GAAIPEAKITATLVEQDVSRSTKSNEEGYYIFNAMPPGTYRLEAEQSGFQRLVHSEIQ
                     LTVSQNLRVDLGMRLGRVSETVEVTASALLVDTLSPALSSLVDDRRVVDLPLNGRNVI
                     SLASTLPGVLGVLAPQQLTDARSGPLMNVNGGIATQNLFTFNGGIFVNPSRNTGLIYP
                     PPDALQELSIQTQNFTAEYGRNAGSQVNVVSKSGTNEIHGALWEFLRNDNLNARNFFA
                     SRVPGKIQNQFGFAAGGPIRRNKLFIFGSYQALRYRAEALGVSLNVPSDAVRAGDFRE
                     LSTTLKNPVDTLTG"
     gene            2099..4351
                     /locus_tag="VFQ79_23615"
     CDS             2099..4351
                     /locus_tag="VFQ79_23615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142731.1"
                     /translation="MQGNIVSPGCITDMAKNLLQMIPSSPTGRVNTLDPAPQNGGLTL
                     IRGDWHQSDRHIVSAHVFLDRNSRTRPQFAGGNVPGYLGSFLNQGTTMATLNDTFTFS
                     PTVLTQSTASFLRSASLSNVTQTVTHDKIGVNLPFYPENGRLGVTIGDINFGTASRVL
                     FVSNTWQFRNMTTVVRGRHSFKFGGEWLHPTFLQIFLGGTGMNFNGSRTGDAMADFLV
                     GAFRQVSGGFGVRTNDNIQDAPALFFQDDFKLHPRLTLNYGVRWEPYFPWVDKYDRLT
                     SLAKIGTDTRSAKFPDAPPGLLFAGEPGVPRGISGPDKNNFAPRFGLAWDVFGNGRTS
                     VRTSYGVFYDAIKGTSVAQEAAPWAGNFQIFDGRAADPFGSLGLTPPPVTPAGEGFGC
                     VNTTTFPGVRCDRFPLPIAGLFMGRNIRTPYIQSWSFAIQQQVASDIMVQATYLGKIG
                     TKLEGFSSVNPARYVNDPITGEPPSLQNVNNRVITAPGILAPNVLELQSKFRSWYHAF
                     QAQAVKRFSRGMSFSGSYSLAKSIDITNLNVGGWSLSNPFDVRANRGRSNFDRRHAFV
                     TSWLWSPTWKGRNAWQRFLFEQWTLTGIHTLQSGAPFTIFMGDDVAQDGVSGSQQHGM
                     LKPGAVVAREHANRGDMIAKFFNTDAFVPTNEVPRGVYGDSGRNILSGPGASNTDFSA
                     IKDFSVGERFRVQFRSEFFNIFNQVRLGCIDTTWGCSEPSSTVNSTSFGQIRSAGAAR
                     EIQFALKLLW"
     gene            complement(4875..5084)
                     /locus_tag="VFQ79_23620"
     CDS             complement(4875..5084)
                     /locus_tag="VFQ79_23620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142732.1"
                     /translation="MSGLEAMTDEQFERHALEVLQRELGVDGLARFLRLDRSGSRDYT
                     KDRMQWQKDLTVEQIIDSIKRRRRS"
     gene            complement(5081..5530)
                     /locus_tag="VFQ79_23625"
     CDS             complement(5081..5530)
                     /locus_tag="VFQ79_23625"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0142733.1"
                     /translation="MSMKVYLDACCLNRLTDDQTQLRIRQEAEAVERIFKRMRHGTVQ
                     WISSEALLDEIDKNPDVERRLENAALLALASENVEIDDSIASRAVDLQMAGYGAYDAV
                     HLACAEAAQVDVLLTTDDGFIRRASRQDGKPLVAVRNPLFWSKENLI"
     gene            complement(5616..6101)
                     /gene="csb2"
                     /locus_tag="VFQ79_23630"
     CDS             complement(5616..6101)
                     /gene="csb2"
                     /locus_tag="VFQ79_23630"
                     /inference="COORDINATES: protein motif:HMM:NF021149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HEU0142734.1"
                     /translation="MVIAPFGHETALNHLADQLDGVQLHPEDGAVGPVLERLRGDAVT
                     ALYLRPSHVWATVTPAILPGHDDHKPAKTIKLIERSLRQSGIDHTCRFSWSALPNFTN
                     CLTAHKYDRQKRRVGYYRPQYLEKFTAVHLRIAFERPVAGPLCVGAGRHCGFGVLASL
                     K"
     gene            6504..6989
                     /locus_tag="VFQ79_23635"
     CDS             6504..6989
                     /locus_tag="VFQ79_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142735.1"
                     /translation="MAYRANPFLDRMSERTTSDQEFVRLFSPKILERLAEDAFEGALH
                     IFRSPPGGGKTTLLRAFTPTALRAFWNARRVQEMSESYQRLSARQILDEHSGPQMLGV
                     LLSCASGYADLPPGATVAQEGLFRALLDCRVVLRSLRSLATLLGFSSPKRIRSTKRRA
                     I"
     gene            6998..>7203
                     /locus_tag="VFQ79_23640"
     CDS             6998..>7203
                     /locus_tag="VFQ79_23640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142736.1"
                     /translation="MLVNVPSARMRSTLVAAMSEVYVGHFALAAEKQGNPARAFEILE
                     RARGRAAADALRALPLQQPRRDGE"
BASE COUNT         1484 a         2239 c         2033 g         1447 t
ORIGIN      
        1 cggcacctac gatccgcaac tgggactcct ctattggccg accggtaatc cctgcccgga
       61 tttcaacggc gatgagcgaa agggcgacaa tctttattcg gattccgtgg tggctctctc
      121 cgtcaagacg ggtcagttga aatggtatta ccagttcacg ccgcacgatc tgtgggattg
      181 ggatgcgcaa caacctccgg tgctggtgga tgtggaatgg aaagggcgtc cgcgtaaact
      241 tcttcttcat gcaaatcgaa acggcttcct ttacgtgctc gataggatca gcggagaatt
      301 gctgttgggc gaaccgttcg taaagaagct cacctgggcc tccggcatcg gcaaggatgg
      361 acggcccatg aagattccag gacaatcgcc ttcacctgaa ggcacgaagg tctgccccgc
      421 cgtggaagga gcgacgaact ggttttcgac cagctttcat ccggacactg gactgtatta
      481 cgtccaaacg ctggagaaat gcaacatctt tgtgaaagaa gttggcaagt gggagccggg
      541 caagtcgtac tacgacggtt ccacccgtca ggtgccgggc gagccgggcc agaagatttt
      601 gcgggccatc gatattcgca ccggcaaaat cgcctgggaa ctgccgcagg ccggcgatgc
      661 gaacacttgg ggaggcaccc tggcgaccgt ttccggactg gtattttttg gagacgattc
      721 caacaacttc agtgcggccg atgcaacgac cggaaggctg ctatggagtt tccccgcgaa
      781 tcatctctgg aaagcttcgc ccatgacgta catcttcgac cacaagcagt acgttgccgt
      841 tgcttcaggg cctaatgtga tcgcattcgc gttagtggaa tgaagacctc tggctttccg
      901 attgattttt aatcgaaatc atttaagagt gtaagacctt gaattaacga tagggtgtat
      961 actgctttcc ggaggaaaga gatccatgag cggttctgtt catggaccaa ggagtttccc
     1021 aatgcttgga acgaagctgt ttcgcgcttg tgaagccgca ctgcttatca tcggactgct
     1081 cgctttatcc gcccccgcca ccctggaggc acaaacaacc ggcaccatca gcgggttcgt
     1141 aacggacccg agtggcgcgg caatacccga ggccaaaatc accgccacac tcgtggagca
     1201 ggacgtctcg cgctccacaa aatcgaatga agaaggctat tacatcttca acgccatgcc
     1261 tccgggcacc taccggctgg aagcggaaca gagcggattc cagcgactgg tccatagcga
     1321 gatccaactc accgtgagcc agaacctccg ggtcgacctc gggatgcggc ttggccgagt
     1381 ctccgaaacc gtagaggtga ccgcctccgc gctgctcgtc gatacgctct cgcccgccct
     1441 gtcgagcctt gtggacgacc gccgcgtggt agacctaccc cttaacggcc gcaacgtcat
     1501 ctccctcgcc tccaccctgc ctggcgtgct gggcgttctc gcaccccagc aactcaccga
     1561 tgcgcgcagc ggcccgctta tgaacgtgaa cggcggaatc gccacccaga acctgttcac
     1621 gttcaacggc ggtatattcg tgaatccctc gcgcaacacc ggccttatat atccgccgcc
     1681 cgatgccctt caggaactca gcattcagac ccagaatttc accgctgagt atgggcgcaa
     1741 tgccggctcc caagtgaacg ttgtttcgaa gtccggcacc aacgagatcc acggcgccct
     1801 ctgggagttc ctacgcaacg acaatctgaa cgcgcgcaac tttttcgcct cccgcgtgcc
     1861 cggcaagatc cagaaccagt tcggctttgc cgccggcggc cctattcgcc gcaacaagct
     1921 cttcatcttc ggctcctacc aggcgctgcg ctaccgcgca gaagcgctcg gcgtctcgct
     1981 taacgtgccc tccgacgccg tccgcgcggg cgatttccgc gagttgagca caaccctgaa
     2041 gaacccggtg gacaccctga caggctagcc gttcaccgac tctaccggca ggccctgtgt
     2101 gcagggcaac atcgtcagcc cgggctgcat caccgacatg gccaagaacc tgctgcagat
     2161 gatcccgtca tctcctaccg gccgcgtcaa cacgctcgac ccggcgcccc aaaacggcgg
     2221 tctcaccctc atccgcggcg actggcatca gagcgacagg cacatcgtgt ccgcccacgt
     2281 gttcctcgac cgcaatagcc gcacccggcc ccagttcgcc ggcggaaacg tgcccggata
     2341 tctggggagc ttcctgaacc aggggaccac catggccacc ctgaacgaca ccttcacgtt
     2401 ctctccgacg gtattgacgc aatccaccgc cagctttctg aggtcagcgt cgctctccaa
     2461 cgtcacccag accgtcacgc atgacaagat cggcgtcaat ctgcccttct atccggaaaa
     2521 cggacgcctc ggcgtgacca tcggcgacat caacttcggc accgccagca gggtgttgtt
     2581 cgtcagcaac acgtggcagt tccgcaacat gaccaccgtg gttcgcggca ggcacagctt
     2641 caagttcggg ggcgagtggc tgcacccgac gttcctccag atcttcctcg gcggcaccgg
     2701 aatgaacttc aatggaagcc gcaccggcga tgccatggcc gacttcctgg tgggcgcgtt
     2761 ccggcaggtg agcggcggct tcggtgtccg aacgaacgac aacatccagg acgctccggc
     2821 cctcttcttc caggacgatt tcaagttgca tccgcggctc accctgaact acggcgtcag
     2881 gtgggagccc tacttcccct gggtggacaa gtacgaccgg ctgacgagcc tggccaagat
     2941 tggcaccgac actcgctccg cgaagttccc ggatgcgccg ccgggacttc tcttcgccgg
     3001 cgaaccgggc gtccccaggg gcatcagcgg gccggacaag aacaatttcg ctccccgctt
     3061 cggcctggcg tgggatgtct tcggcaatgg caggaccagc gtacgcactt cctacggggt
     3121 tttctacgat gccatcaagg gaacttccgt cgcccaggag gccgcgccgt gggccgggaa
     3181 cttccagatt ttcgacggcc gtgcggccga tcctttcggg tcgctcggcc tcactccccc
     3241 tcccgtaacg cccgccggcg agggcttcgg ctgcgtcaac accaccactt tcccgggcgt
     3301 tcgctgcgac cgcttccccc ttcccatcgc gggcctcttc atgggccgca acatcagaac
     3361 gccctacatt caaagctgga gttttgccat ccagcagcag gtggcttccg acatcatggt
     3421 tcaggctacc tacctgggaa agatcgggac caagctggag gggttctcga gcgtcaaccc
     3481 ggcccgttac gtaaacgacc cgattaccgg cgagccgccg tcgctccaga acgtcaacaa
     3541 ccgcgtgatc acggcccctg gcatactggc gccgaatgta ctcgaactgc aaagcaagtt
     3601 ccgcagttgg tatcacgcct ttcaggcgca ggctgtcaag cgcttttccc ggggaatgtc
     3661 gttctcgggc tcctattcgc tggcaaagtc cattgacatc accaacctca acgtcggagg
     3721 ctggagcctc tccaacccgt tcgatgtccg cgccaaccgg ggacggtcca actttgaccg
     3781 gcgccacgcc ttcgtcacct cctggctctg gtcccccaca tggaaaggca ggaacgcgtg
     3841 gcagagattc ctattcgagc aatggacgct caccggaatt cacaccctgc agagtggcgc
     3901 gccgttcact attttcatgg gcgacgacgt cgctcaggac ggcgtcagcg ggagccagca
     3961 gcacggcatg ctcaagccgg gtgcggttgt cgcccgggag cacgcgaacc gcggcgatat
     4021 gatcgccaag tttttcaaca ccgacgcctt cgtccctaca aacgaagttc cgcgcggtgt
     4081 ctacggcgat tcggggcgca acatcctgag cggacccgga gcgagcaaca ccgacttttc
     4141 cgccatcaaa gacttttccg tcggcgagcg ctttcgcgtt cagttccgtt ccgagttctt
     4201 caacatattc aaccaggtgc gcctgggctg catcgatacg acctgggggt gttcggaacc
     4261 ctccagcacg gtgaactcga ccagcttcgg ccagatccgg agcgcgggtg ccgcgcgcga
     4321 gattcagttt gccctgaagc tgctgtggtg aagatgagac ggtagcaata aagcatcgga
     4381 gcactgatgc cggtaccgga tgccgcatag ctgaattccg cgtcttacga ggccgcgaag
     4441 gcgagttggc ttttcttgtc ccatggctga ggcaagccgc tgccccactg acgttcgggc
     4501 gattgtctcc agacgcggtg aacgcaactc cctccgtcac gctgcgacat tatcaaaagt
     4561 cgattccagc aagcatccaa gctgcagcgg tagcgttgga gtcagagttg atcggcaaaa
     4621 gcagccaaat tgattcgaac aggttttgaa caggtcagaa gccgaaggga tgagtcaaaa
     4681 tgactaaaaa ctggagccac ccgccgggat cgaaccggcg acctgctgat tacgacaaat
     4741 atggccgacc tctaatctct ccattcactc cataccgaag tgcctgatat caacaacttg
     4801 gggaagctgc tctcgctcaa ccagcaactc caggcaccgc aaatggatag ggttttggca
     4861 tagtttggca cagatcaact gcgccggcgg cgcttgatgg aatcaatgat ttgctcgacg
     4921 gtcagatcct tctgccattg catccgatct tttgtgtagt cgcgcgaccc cgaacggtcc
     4981 aatcgcagga aacgcgccaa tccgtcgacg cccagttcac gttggaggac ttccaaagcg
     5041 tgccgttcaa attgttcgtc ggtcatcgcc tcaaggccac tcatatcaaa ttctctttac
     5101 tccagaatag cggattccgg acggcaacaa gcggtttgcc atcctggcgt gaggccctgc
     5161 gaatgaatcc gtcgtccgtc gtgagaagaa cgtccacttg cgctgcttct gcgcacgcca
     5221 agtgcactgc gtcgtaagct ccgtagccag ccatctgcag atctacggcg cggctggcga
     5281 tggaatcgtc tatttcgacg ttctcggacg cgagggccag cagagctgca ttctccagtc
     5341 gtctttcgac gtcgggattc ttgtcgatct catcaagcag cgcctcactg gagatccatt
     5401 gaaccgtgcc gtgccgcatc cgcttgaaga tgcgctcaac agcctcggct tcctgtcgga
     5461 ttcgaagctg agtctgatca tcggtgaggc gattcagaca acaggcgtcg agatagacct
     5521 tcatgctcat cgtcgctgcg cgaggaatgg cgctttgtcg cagccaatcc gcgtgtcagg
     5581 ggccagtatc tgagaaagaa gcaagagaag ccaagtcact tcaatgatgc caaaacgccg
     5641 aagccacaat gccgtccagc cccaacgcac agcggtccgg caacgggacg ttcaaaggcg
     5701 atgcgcagat gaactgccgt aaatttttcg agatactgag gacgataata cccgacccgc
     5761 cgcttctggc ggtcatactt gtgtgcagtc agacagttgg taaaattcgg caaagcactc
     5821 caactgaacc tgcatgtatg atcgattcca ctttgccgga gtgagcgctc gataagtttg
     5881 atggtcttgg caggtttgtg gtcgtcatga ccgggaagaa ttgccggcgt cacagtggcc
     5941 catacatggg acgggcgcag gtatagggcc gtcacggcat cgccccgcaa tcgttcgagc
     6001 accggtccaa cggcgccatc ctcaggatga agttgaacac cgtcgagttg gtcagccaga
     6061 tggttaagag cggtctcatg gccgaacggc gcaatgacca tcaggcgagc cgcgtccgac
     6121 tagtgagttt ctcagccacg tcacgcgcat aacccgccgc ctagaagcat tgggtgcacc
     6181 cattgaaacc attcgaacga cgcaccctta tcaggtcagt gttcgcttcc gggccggggt
     6241 cgataccggg agcaattggt ttgtcattgc ggatgctctc aggcaagcgg ggcttgacat
     6301 ctcgagggtg ggccgaatcg ttggtagtgg tcatacttga aatcagcaag caactggatg
     6361 gatggaacgt acctgctcac ctccgcttcg tcgtgccagc ccataacttc ggcgagcagt
     6421 tgttgcgcgt tctggtctct catcgtgtcg ccgccccgag ttcttcagag agcctaaggg
     6481 taagccgaca tcgggagttt taaatggctt atcgtgcgaa cccatttctg gaccggatgt
     6541 ccgagcggac gacctcggat caagagtttg tgcgcctctt ctcgccgaag atactggaac
     6601 gattggccga ggatgcgttc gagggcgcgc ttcacatctt ccgaagcccg ccgggcggag
     6661 gcaaaaccac cttgctgcgc gccttcacgc cgacagcgtt gcgggccttc tggaacgccc
     6721 gccgcgttca ggagatgagc gagtcctacc agcgcctgag tgcccgacag attctggatg
     6781 agcattcggg cccgcagatg ctgggcgtgc tcttgtcctg cgcgtcagga tatgccgatc
     6841 ttccgccggg cgcgaccgtt gcccaggagg gccttttccg tgccctcttg gactgccgcg
     6901 tcgtgcttcg atcactcaga agcttggcga cgttgcttgg cttctcctca ccgaagcgga
     6961 ttcgctctac gaagaggcgg gcgatctgat cgaggctatg ctcgtcaatg ttccgagcgc
     7021 gagaatgcgc agcacgctgg ttgccgcgat gagcgaggtc tatgttggcc acttcgcact
     7081 cgcggctgag aagcagggca atccagcaag ggcctttgaa atcctggagc gtgcgcgcgg
     7141 acgagccgcc gcggacgcgc tccgcgccct gcctttgcaa caaccgcgcc gagatggaga
     7201 aaa
//
LOCUS       DASTMK010000578         8926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_605744, whole genome shotgun sequence.
ACCESSION   DASTMK010000578 DASTMK010000000
VERSION     DASTMK010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605744"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2985
                     /locus_tag="VFQ79_23645"
     CDS             <1..2985
                     /locus_tag="VFQ79_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142737.1"
                     /translation="EAKGFRQVATSVFPVAAYRTVRQDLNFALATAATEVTVVETVSP
                     VTQLESPSINATLTTKQILELPTTLRSVYNAAGDSSLIFTIMPLTIPGVNQVSNGAKW
                     LVPGGGSVGMKLKVDGIETNFGLFGSPDPVSQPSMESVQEFTANILTNRAEFGGLGTI
                     TTVTRSGANDLHGDVFWHLRNSALDARNAFATSKPFQNIHNYGVSAGGPLIKDKTFWY
                     GTWDETRGSRAYLFTANVPTVAERAGDFSGAAALRNPYTGMNPIGDGNRILPQYISPQ
                     AQRAQEILFPLPNFGPPDLRAGNYRASFNGPELHQLFEIRLDHNFASGHSIFARYQHK
                     DTSYDIPGARTQLPPSSVGTSNNTRVVNFWTLGDVYSIRPNIVNEFRAGLVILVSESS
                     ADVTGQPLLDRIGIRGLPDRTGVEGVPNMSITGYTSVGQLLLNPVNDGHGQFSNNLTW
                     IKGRHSLKFGVEMVRWFVNRHLPAENALFGNFNFTNRFTGNPYADFLLGLPNAVTRLD
                     PYPSQYFRFTDWNFYGQDDFKVTPKLTLSYGLRYEYNGPASTRDGNIYSFDPASGSIV
                     IPSEDSRRLFSPYWPADVPVITADRLGAGKSLREADKNNFAPRFGFSYQIGNDARTVL
                     RGGWGVYYSHLSANVAAGLAAGPYAISTTSNNNFVDGQPLFTFENPFAAPGAAGSLNV
                     NAVDTRLLNSYAMQYSLSLERQVTQDIGVRISYIGSKGTQVLYRRNINQPEPSTAPFS
                     ASRRPYPRFNTIAWGENGANDLYNGLQTQVQKRFSKSLLFSSTWTWAKSVSEVDDTGD
                     FELNTSIENAYDRARDRSDVYSVPRHQWMNQVLYELPGRGALLGGWQVNVLLNLSTGN
                     FLNPVFSGSDPSNTNTIGGRPDVVKSSADGPRTQAEWFDRTAFAVPPANSARFGNAGR
                     NLIQGPGYVIFNAGIAKSSSLERLGRFQVGVSFQNILNHVNLGQPNMTVNNPVGGTIT
                     STHIFPPAGSPRNGLLSLRWSF"
     gene            complement(3006..3413)
                     /gene="mscL"
                     /locus_tag="VFQ79_23650"
     CDS             complement(3006..3413)
                     /gene="mscL"
                     /locus_tag="VFQ79_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU0142738.1"
                     /translation="MLQGFKQFLMRGNVIDLAVAVVMGAAFGAVVTSLVESLVTPLIA
                     AIVGKPDFSAIVFTINGSRFMIGQFINALVSFLLIATAVYFFIVVPVNALMARMRRGE
                     ATPDPTTKACPECLSDVPIAARRCAFCTSVLTK"
     gene            complement(3462..4523)
                     /locus_tag="VFQ79_23655"
     CDS             complement(3462..4523)
                     /locus_tag="VFQ79_23655"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEU0142739.1"
                     /translation="MKNCFFRGALSLIVLLAGTLRADQVTLKNGDRITGAIVKKDGKE
                     LTIKSDLFGVVTVPWEQVESVKSETPLNVVLSSGQTVQTPIATSGGKVELTATDKTVE
                     PADVLAIRDTAEQAAYDRLQNPGWVDLWAGTATLGFAGTQGNARTKTLTTALNAARLT
                     RNDKTTIYFSAIKASALVEGVSAGTAQAVRGGLGYDHDVSPKLFVNVFNDYEYDRFQD
                     LDLRFVLGGGLGYHAWKTERGRLDLAAGMAYNHSRFGPPAPAAAFSRNAAEAYWGNDF
                     SYALTSATSLVQSYRMFNNLSDTGEYRINFDLGAVTKITSWMTWNIGLSNRFLSNPVA
                     GRQRNDFLYTTGLGVTFAR"
     gene            complement(4664..5119)
                     /locus_tag="VFQ79_23660"
     CDS             complement(4664..5119)
                     /locus_tag="VFQ79_23660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0142740.1"
                     /translation="MQKKSTVRTTPQSAEVLVVDDDEQVLNMILFMLRRHKLEAKGAR
                     NSAAALQLVKDHPHAIRAAIINLFLPEISGAELAVRITADYPEIGIVIISGVPLEIWP
                     AREREMLSRLPRGFVFARKPVLVDTLLARVRFVQGDVTFLRGYCPGDSA"
     gene            5274..5933
                     /locus_tag="VFQ79_23665"
     CDS             5274..5933
                     /locus_tag="VFQ79_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142741.1"
                     /translation="MKNRKAPAVAKRRSILNPMVNKHDDRPTAEHVSAETAKGRTLAA
                     DEPAAGNVDKIRDILFGSQMRDYDSRFARLEERVERELSDMRDSSKKRFDGLEAFIKR
                     EVESLQSRLKTEREERSESVRLLSRDLKDLSDLLSKKISEMDDHVTESQGQIRADILQ
                     QSKDLSDEIERKREEISAALDRRFQQLRTEKTDRAMLAELLTEVALRLKNEFQVPTAA
                     D"
     gene            5953..7515
                     /locus_tag="VFQ79_23670"
     CDS             5953..7515
                     /locus_tag="VFQ79_23670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0142742.1"
                     /translation="MSRETAANGRPLTSELAELRELLVGPEQAAIEALRERFDNPKLF
                     AEAVGAVLAEALRRRASGDPRFRQSLERIVEDVIRNSVRRNPAILTDALLPVISGVVR
                     KSVAAALRGMMESIDQLVEQGFSRRAFEWRIEAFRTGRPFGEIVLLQSTLYRVEQVFL
                     IHRKTGLLLSQRAAGGAVHRDADLIAAMMTAIQDFVTDSFGREEGENLESFRVGECAV
                     WIESSPNAALAGVVRGVAPARLRRVFQGALERICSEQAREIEEFDGDTACFQDSERHL
                     DSCLLGRLSAAESQRRALWPWVLAAGVFVLAVILWSVPVLRERSRWSQYLNRLKEEPG
                     IVLTEAGRAGGKYYVSGLRDPLAADPDALLRESGINGGQVRFQWEPYVSLQPGLAAAR
                     RMETAKAAIEKQTVRFPVNSARIAAAQAETITGLVSHIRDLLESARVIARPVLVEVIG
                     HADFSGDENHNNRLAGERADAVLAVLVNEGLPAPQIQPRGAGSSEPLRPGDSELDKEF
                     NRSVSFRVLFPK"
     gene            7512..8063
                     /locus_tag="VFQ79_23675"
     CDS             7512..8063
                     /locus_tag="VFQ79_23675"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HEU0142743.1"
                     /translation="MNPADHPSNAIQKKVCMVGAFAVGKTSLVKRFVESIFDERYHTT
                     IGVKIDRKHLTVKGREVTLILWDLAGDDELAQLRMSHLRGASGYILVADGSRPATLDK
                     AIELQSRVESTLGSVPFVLALNKADLVHEWRIDAGAVAALDQSGWTIFNTSAKVGTGV
                     ERLFETLAAKMLEATGKQVSDAT"
     gene            8053..>8926
                     /locus_tag="VFQ79_23680"
     CDS             8053..>8926
                     /locus_tag="VFQ79_23680"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0142744.1"
                     /translation="MQPECFSGFDLFSALDMLALERGQSGCFVPLGSLNATFQSLGIS
                     ESWNPGETFPYLNDFLAGADRVWAEGGRIDSDVWVQSDAQGREHALEASAIGAENRSF
                     LVLRVLGSEYEERRNAIQKARESRLLYERLQEIARELAAARDELGRRNREMERLDRLK
                     SEFVASMSHELRTPLNAILGFSSLLAEDSQGTLIPEQREFVQHVQRGAQHLLKLINDI
                     LDLSKIEAGYLKLDYETFPLADSLTEVLSTVRPLAGKKRIRLGVEQDSSCVVHADRRR
                     FVQILLNLLSNAIAF"
BASE COUNT         1845 a         2543 c         2725 g         1813 t
ORIGIN      
        1 gaggcgaaag gcttccggca ggtcgcaacg agtgtctttc ccgtcgcggc ataccggaca
       61 gtgcggcagg atctcaattt tgcgctcgcc acggcggcca cggaagtcac ggttgtagaa
      121 actgtctcac ctgtgactca actggaatcg ccgtccatta acgcgacgct tacgacgaaa
      181 cagatcctcg agttgcccac tactctgcga agcgtctaca acgccgccgg ggattcgtcg
      241 ctgatcttca cgattatgcc gctcacgatc ccgggagtga accaggtttc aaacggggca
      301 aaatggctgg tgccaggcgg cgggtcggtg ggaatgaagt tgaaagtgga cggcatcgag
      361 acgaatttcg gtttattcgg cagcccggac ccggtatcac agccatcgat ggaatccgtt
      421 caggaattca ccgcgaacat tcttaccaac cgcgccgaat tcggcgggct ggggaccatc
      481 accacggtga cacgttcggg cgccaacgac ctgcacggcg atgtcttctg gcatttgcgc
      541 aacagcgccc tcgacgcccg gaatgccttc gccaccagca agccctttca gaatatccat
      601 aactatggcg tttcggccgg tggtccgctc atcaaggaca agactttctg gtacggcacg
      661 tgggatgaga ctcgcggctc gcgggcgtat ctgtttaccg cgaacgttcc gacggtcgcg
      721 gagcgcgccg gcgatttttc gggcgccgcg gcattgcgga atccatatac ggggatgaac
      781 cccattggcg acggcaaccg tattttgccg cagtacatca gcccccaggc gcagcgggcg
      841 caggagattc ttttcccgct gccgaacttc ggcccgccgg atttgagggc ggggaactac
      901 agggcatcgt tcaacggacc ggagctgcac cagctttttg agattcggct ggaccataat
      961 ttcgccagcg ggcattccat cttcgcccgc tatcagcaca aagatacgag ctacgatatt
     1021 ccgggcgcgc gcacgcagct tcctccgtcg tccgtcggca cctccaacaa cacgcgcgtc
     1081 gtaaatttct ggaccctcgg cgacgtgtac tcgattcgcc cgaacatcgt gaacgaattt
     1141 cgcgccggat tggtgattct ggtttccgag tcgagcgccg atgtcaccgg gcaaccgcta
     1201 ctcgatagga tcggcatccg cggtctgcca gaccgcaccg gagtggaagg cgttccgaac
     1261 atgtcgatca ccgggtacac cagcgtcgga caacttctgt tgaacccggt gaatgacgga
     1321 catggacagt tcagcaacaa tctgacctgg atcaaagggc gtcattcgtt gaagttcggc
     1381 gtcgagatgg tgcgatggtt tgtcaaccgc catctgcccg cggagaacgc gctgtttgga
     1441 aatttcaact tcacgaaccg gttcacgggc aatccttacg cggatttcct gctcggattg
     1501 ccaaacgcgg tgacacgcct cgacccttac ccttcacaat atttccggtt caccgattgg
     1561 aacttctacg gtcaggacga cttcaaggtc actcccaagc tgacgctgag ctatgggctc
     1621 cggtatgagt acaacggtcc ggcgagcacg cgcgacggca acatctactc tttcgatccc
     1681 gcatcgggtt cgatcgtaat tcccagcgag gattcgcggc ggctgttcag cccctattgg
     1741 cctgccgacg tgccggtcat tacggcggat cgactgggtg cgggcaagag tctgcgcgaa
     1801 gcggacaaga acaactttgc gccacggttc gggttttcct atcagatcgg caacgatgcc
     1861 cgcacggtac tgcggggtgg atggggtgtg tactattcgc acctgtcggc gaatgtcgcg
     1921 gccggtctcg ccgccggacc gtatgcgata tcgaccacca gcaacaacaa cttcgtggac
     1981 ggccagccgc tcttcacgtt cgagaacccg ttcgcggcac ccggcgcggc cggatcgttg
     2041 aacgtgaatg cggtggatac gcgcctgctg aacagctacg ccatgcagta cagcctttcg
     2101 ctggaacggc aggtcacgca ggatatcgga gttcgcatca gctatatcgg ctcgaagggc
     2161 acacaagtac tctacaggcg aaacatcaac cagccggagc cctctacggc gccgttctcc
     2221 gcatcgcgcc ggccctaccc gcgcttcaat accatcgcct ggggtgagaa cggcgcgaat
     2281 gacctgtaca acggcctgca aacacaagtg cagaagcgct tttcaaagag cctgctgttc
     2341 agctcgacct ggacctgggc caagtccgtc agcgaagtgg acgatacggg cgattttgag
     2401 ctgaacacgt ctatcgagaa cgcgtacgac cgggcgcgcg accgctccga tgtctattcg
     2461 gtgccgcgcc accagtggat gaaccaggtt ctctatgaat tgcccggtcg cggtgcgctc
     2521 ctgggcgggt ggcaagtcaa cgtgctgttg aatcttagca ccgggaattt tctgaatccg
     2581 gtgttctccg gttccgaccc ctcaaacacg aacacgattg gcggaaggcc cgatgtggtg
     2641 aaaagctccg ctgacggacc acgaacgcag gcggaatggt ttgatcgtac ggcttttgcc
     2701 gtgccgccgg cgaactccgc acggttcgga aacgccggcc gcaacctgat tcaggggccc
     2761 ggttacgtca tattcaatgc gggcattgca aagagctcca gtctggagcg gctcggaaga
     2821 tttcaggttg gagtctcgtt ccagaacatt ttgaatcacg tgaacctggg ccagccgaac
     2881 atgaccgtaa acaatccggt cggcggcact atcaccagca cgcatatttt cccgcccgcc
     2941 ggaagcccca gaaatggctt gttgagcctt cgctggagct tttagcaccg gcgagcgcac
     3001 acttcctatt tcgtcagaac cgatgtgcag aaggcgcaac ggcgcgctgc aatcggaacg
     3061 tcgctcaggc attcgggaca ggccttggtg gttgggtcgg gagtcgcttc tccacggcgc
     3121 atacgcgcca tgagagcgtt cactggaacc acgataaaga aatacaccgc cgtcgctatc
     3181 agcaggaacg acaccagcgc gttgatgaac tgcccgatca tgaaccggct gccgtttatg
     3241 gtgaacacga tcgcggaaaa atcgggcttg cccacgatgg ccgcaatcag cggtgtgacc
     3301 agcgactcga ccaatgatgt cacgaccgcc ccaaacgccg cgcccatgac cacggcgaca
     3361 gcgaggtcga taacgtttcc acgcatcaga aactgcttga aaccttgtaa cattttgttt
     3421 ctccttatag aatctgaacg gctgaatttc cctaagtgca gctacctcgc aaatgtgacg
     3481 cccaagccgg tcgtgtagag aaagtcgttc cgctggcgtc ccgccaccgg attgctcagg
     3541 aaccggttgc tgaggccaat gttccaggtc atccagctcg tgattttggt gaccgctccc
     3601 aggtcgaagt ttatgcggta ctcgccggta tcggagaggt tgttgaacat ccggtacgac
     3661 tgcaccaggg atgtggcgct ggtgagcgcg taactgaaat cgttgcccca gtaagcttca
     3721 gcggcattcc gggaaaaagc cgcggcggga gcggggggtc caaagcgcga gtgattgtag
     3781 gccataccgg cggctagatc gaggcggcct cgttcggtct tccaggcatg ataacctaaa
     3841 ccacctccca ggacgaatcg gaggtccaga tcctgaaacc ggtcatactc gtaatcgttg
     3901 aatacgttga caaaaagctt cggcgaaacg tcatggtcat accccaagcc gccgcgcacg
     3961 gcctgcgccg tgcccgcgct gactccttca actagcgccg aagccttaat ggcgctgaag
     4021 tagatcgtgg tcttgtcgtt acgtgtcagg cgggcggcat tcaacgcggt ggtgagcgtc
     4081 ttcgttcgcg cgttcccctg cgtgccggca aaaccgagcg ttgccgtacc cgcccaaagg
     4141 tctacccatc cggggttctg gagcctgtca taggcagcct gttcggccgt gtcacgaatt
     4201 gctaaaacat cggccggctc cacagtcttg tcggtggcgg tcagctccac tttgccgccg
     4261 ctggtcgcga tgggtgtttg aaccgtttgt ccggacgaga gaacgacgtt gagcggggtt
     4321 tccgatttga ctgactccac ctgttcccac ggcacagtaa ccacgccaaa taagtcgctc
     4381 ttgatcgtca gttctttgcc gtccttcttc acgatcgcac ctgtgatgcg atccccattt
     4441 ttaagggtga cctggtctgc gcgcaaggtg ccggccagca gcactattag cgagagtgct
     4501 cctctgaaaa agcaattttt catagatgtt caagctccaa tccggattga tcttaatgtt
     4561 cctcttccac catttccagg agttttccaa agtgatacgc caaaacgcga atatctcagg
     4621 cttgagacga ttttcggccg gaattcggcg ggtgtacctt gtgtcacgca gagtcgccgg
     4681 gacaatagcc acgcagaaat gtgacatccc cttgcacgaa ccggacccgt gcaaggagcg
     4741 tatccaccag gacaggtttc ctcgcgaaga cgaatcctcg cggcagccgt gatagcattt
     4801 ctctttccct cgccggccaa atctccagcg gtactccaga gataataaca atcccgatct
     4861 ccggatagtc cgcggtaatg cgaaccgcca actccgcgcc cgaaatttcc ggcaggaaaa
     4921 ggttgatgat cgcggcccgg atggcgtgcg gatgatcctt taccagttgg agagcggccg
     4981 ctgagttccg ggcgcctttg gcttcgagtt tgtgccgccg gagcatgaag agaatcatat
     5041 tgagcacctg ctcatcgtca tcgactacca gtacttcggc ggattgcggt gttgttcgaa
     5101 cggtagactt cttttgcatc ggcgccttct tccttgcacg ccagatgcct gaacgcaatg
     5161 tgacgccgct tgctgacgcg cgcggctcgg taatgagcgg ttgcgttgaa actggagcta
     5221 tgatgtttca caggcgaacc taccggttcc gctggtaggg agggaggggt acggtgaaga
     5281 atcggaaggc gccggcggtc gcgaagcgaa ggagtatatt gaacccgatg gtaaacaagc
     5341 atgacgaccg cccaacggcg gaacacgtga gcgcggagac cgcgaaagga cgcacccttg
     5401 cggctgacga gccggctgcg ggaaacgtcg acaagatccg ggatatcctc ttcggatcgc
     5461 agatgcggga ttacgacagc aggtttgcgc gtctcgaaga gcgcgtcgag agagagctgt
     5521 cggatatgcg cgactcttcc aagaagcggt tcgatggctt ggaggcgttc atcaagcgcg
     5581 aggtggaatc tctccagtcc cgtctgaaga ccgaacggga ggagcggtcg gagagcgtaa
     5641 gacttctgtc ccgcgacctg aaggatcttt cagatctgtt gagtaagaag atttcggaaa
     5701 tggacgatca tgtcacggaa tcacagggcc agatccgggc ggatatcctc cagcagtcga
     5761 aagatctcag cgacgagatc gagcggaagc gggaagagat ttcggcggct ctggaccgcc
     5821 gttttcagca gctccgcacg gagaagacag atcgcgcgat gcttgccgag ttgctgacgg
     5881 aagtggccct gaggctgaaa aacgaatttc aagtcccaac cgcggcggac taggctgccg
     5941 ggcagcaccg taatgtctcg agaaactgcg gcgaacggac ggccgttgac cagtgagctt
     6001 gcggaattgc gcgaactgct ggtcggtccc gaacaggcgg ccattgaagc cctgcgggag
     6061 cgcttcgaca atcctaagct ctttgccgag gcggtcggtg cggtgctggc cgaggccctt
     6121 cgtcggcggg ccagtggaga tccgcgattc aggcaatcat tggaacgcat cgtcgaagat
     6181 gtgatccgga attccgtgcg gcgcaacccg gcgattctca ccgatgcgct gcttccggtc
     6241 atctctggag tggtgcggaa gtcggttgcg gcggcgctgc ggggaatgat ggaatccatc
     6301 gaccagttgg tcgagcaggg tttttcgcgg cgcgcgttcg agtggcgaat tgaagcgttc
     6361 cgcacgggca ggcccttcgg agaaatcgtt cttctgcaaa gcacgctata ccgggtagag
     6421 caggttttcc tcattcatcg taagacgggg cttctactgt cgcagcgggc ggcgggcggt
     6481 gcagtccatc gggatgcgga tctgatcgcg gccatgatga ccgccataca ggacttcgtt
     6541 accgactcgt ttggaaggga agaaggcgaa aacctggaat cctttcgagt cggtgaatgc
     6601 gccgtatgga tcgagtcgag ccctaacgca gctctcgccg gtgtggtccg cggtgtggct
     6661 ccggcccggc ttaggagggt atttcaaggc gctcttgaac ggatctgttc cgaacaggcc
     6721 cgggagattg aggagttcga cggagatacg gcttgctttc aggatagcga gcggcatctg
     6781 gatagctgtc tgctgggccg cttatccgct gcggagagcc aacggcgtgc tctctggcca
     6841 tgggttctgg cggccggcgt atttgtgttg gcggtgatac tctggagcgt cccggttctg
     6901 cgtgaaagga gccgctggag ccagtatctg aatcgcctga aggaggaacc cggtattgtt
     6961 ttgacggaag ccggcagagc aggcggcaaa tactatgtct ccggtttgcg ggatccgctg
     7021 gcagccgatc cggacgcgct gttgcgcgag tcgggaataa acggcgggca ggtccggttt
     7081 caatgggagc cgtatgtttc cctccagccc gggctggccg cggcccggcg gatggagacc
     7141 gctaaagccg ccatcgagaa acagactgtg cggtttccgg tcaactcggc gcggatcgcg
     7201 gccgcgcaag ctgagacgat taccggtctt gtgtcgcaca tacgcgatct gttggaatcc
     7261 gcgcgagtga ttgcccggcc cgtgctggtg gaggtaatcg gccatgccga cttctccgga
     7321 gatgagaacc acaacaaccg gttggccggc gagagagccg atgccgtgct tgctgttctt
     7381 gtaaacgagg gtcttccggc gccacaaatc cagccgcgcg gagccggatc gtccgagccc
     7441 ttgcgacctg gtgattcgga actcgacaaa gagttcaacc ggagcgtctc gtttcgcgta
     7501 ctgttcccaa aatgaatcct gcggaccacc catcgaacgc cattcaaaag aaggtctgca
     7561 tggttggagc cttcgccgtg ggcaagacca gcctggtgaa gcgctttgtc gagagcattt
     7621 tcgatgagcg ctatcacacg acgatcggtg tgaagatcga ccggaaacac ctgaccgtga
     7681 agggccggga agtaaccctg atcctctggg acctggcggg agacgacgag ctggcgcaat
     7741 tgcggatgtc gcacctgcgc ggagcttccg gatacatcct ggtggcggat ggctcgcggc
     7801 cggcgacgtt ggacaaggcc atcgaactcc aatcgcgggt cgaatccact ctcggcagcg
     7861 ttccttttgt gctggcgctg aataaggccg accttgtcca cgagtggagg atcgacgcgg
     7921 gcgccgtcgc cgccctcgat caatcgggct ggacgatttt caacaccagc gccaaggttg
     7981 gaaccggcgt cgagcgcctc ttcgagactt tggccgcgaa gatgcttgag gccacaggga
     8041 aacaggtatc cgatgcaacc tgagtgcttc tccggcttcg accttttttc cgccttggat
     8101 atgctggcat tggagcgggg gcaatcgggc tgctttgtgc cgttgggttc gctgaacgca
     8161 accttccaaa gtctcggtat aagcgaatca tggaatccgg gtgagacctt tccctatctc
     8221 aacgatttcc tggccggcgc cgaccgggtc tgggcagagg gcggcaggat cgattccgat
     8281 gtctgggtgc aatccgatgc tcaggggagg gaacacgccc tggaggcatc ggcaattggc
     8341 gcggaaaatc gaagcttcct tgtcctgagg gtgctggggt cggagtacga ggaacgcagg
     8401 aatgcgatcc agaaggcaag ggaatcgaga ctcctgtacg aacggctgca ggagatcgct
     8461 cgcgagctgg ccgccgcgcg ggacgagctc ggcaggcgca accgcgagat ggaacgcctg
     8521 gaccggctga agagcgagtt cgtcgccagc atgagccacg agctccgcac gccgttgaac
     8581 gccatcctcg gcttctcgag cctgctggcg gaagacagcc agggaacgct cattccggag
     8641 cagcgtgaat tcgtccagca cgttcaacgg ggagcgcagc acctgctgaa actgataaac
     8701 gacattctcg atttatcgaa aatcgaggcc gggtatctga agcttgacta tgagacgttc
     8761 cctctggcgg attccctgac cgaagtgctt tccaccgtaa ggcccctggc cgggaaaaag
     8821 cgaatccggc tgggggttga gcaggattcc agttgtgtgg tgcatgcgga ccgaaggcgc
     8881 ttcgtgcaga ttctgctaaa cctcctgagc aatgcgattg cgttta
//
LOCUS       DASTMK010000579         3580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_605837, whole genome shotgun sequence.
ACCESSION   DASTMK010000579 DASTMK010000000
VERSION     DASTMK010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605837"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2165
                     /locus_tag="VFQ79_23685"
     CDS             <1..2165
                     /locus_tag="VFQ79_23685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0142745.1"
                     /translation="MYRNALKKDGLNGEAYYRLGLTGLRMSRPGEASRALRRAVELQP
                     ANVDAAARLADIYLAAYLAQPQHPRELLNEAQQIADRLLKRDPKLFEGLRLAGYLGLA
                     SQDLPKALEYFRQAHEVKPYQPDVSLAMMQTLSAGNQFDEAEKLAKTAIEKNKEYSQL
                     YDGLYLEYLRRNRQAEAEEILKLKIRNNPTSTESRVQLAGFYAATNRRQQMSETLDSV
                     IENRKTLPNALSIVGDFYYRIGEFDQALKLYQHGSESEPAQKLTFQKRMIEVALRQGK
                     REEASRIIDEILAERPNEPDTVAIRAAMTLETGNQDQIREAIKDMESVVKSIPRNAIL
                     RFNLGRAYLATGQTDRARQQLEDAVKMAPAFLPGKLILAELYVRVRNYATARQLADEV
                     LAVNANSTPARLIRSATLMASGDRERARTELLDTLKRQPQSVEAQFQLGVLNYQEGRL
                     KDAETSFTHVRQQAPDNPRATLALAETYLSQGRPQQATELLTAEVSKHPEARDLRIAR
                     ANIAAVRGEYDTAIAEMGELVKQYPESADLYSRLGEYYRRKGDTASALQNLRRARELM
                     PNNASTNLQLALMLEAAGRQSEAMPLYEQVIRIESDNPIALNNLAYIIAESGGDLDRA
                     LTYAQRARQRLPQNADVADTLALIYIKKNLNDNAIQILSGLVAKNPQRADYRYHYATA
                     LAQKGDKLSAKRELEAALRSSPNQQQESKIKELIAKLG"
     gene            2175..2906
                     /locus_tag="VFQ79_23690"
     CDS             2175..2906
                     /locus_tag="VFQ79_23690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HEU0142746.1"
                     /translation="MIEPHRGLQIPERPDSPRKEKPSASRHPSLPYVLPFAAFLVLLV
                     GHQYLELGSWQYPARVAIIALLLIVFSRHVLDFRLSQPVASLGIGVAVFVIWILPDLL
                     WSGYRDHWLFQNGITGTITSSIADHHRMDGWVIFFRSVLAVIIVPVVEELFWRAWLMR
                     WLIKPQFETLKLGAYSAMAFWVTALLFASEHGPYWDVGLLAGIIYNWWMVRTRSLGDC
                     ILAHACTNACLSFYVIFAGKWEYWL"
     gene            complement(2908..>3580)
                     /locus_tag="VFQ79_23695"
     CDS             complement(2908..>3580)
                     /locus_tag="VFQ79_23695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142747.1"
                     /translation="TQKVGTGLLDLSAAIRATVTASPVSISFGASGGTEPLRRNISFT
                     NVGAAADVFSISVESAGAAPTAQVSENSFPLEPGATRTVAVELTGAALEPGEYEGFLL
                     VRGSGVEIRVPYWYAVPSSAPVYIRVLNSTSSGRAGDILRNAITFQVMDKSGIGMAAA
                     DPKVIAESGGGELSNLISADDEIPGAYSADVKLGAGSNVFLIMAGEVSERVVINGRGG
                     VARIP"
BASE COUNT          741 a         1081 c         1071 g          687 t
ORIGIN      
        1 tgatgtatcg caatgccctg aagaaggacg gtctgaacgg tgaggcatac tatcgcctgg
       61 ggttgaccgg cctgcggatg agccggcccg gcgaagcttc acgagccctg cgccgcgctg
      121 tggaattgca gccggccaac gtggatgcgg cggcgaggct ggcggacatc tacttagcgg
      181 cgtacctcgc tcaaccgcag caccccaggg aattgctgaa cgaagcgcag cagattgccg
      241 atcgcctgct gaagcgggat cccaaattgt ttgagggttt gcggctggcc ggctacctgg
      301 ggctcgccag tcaggacctg ccgaaggctc tcgagtattt ccggcaagcg cacgaggtca
      361 agccgtacca acctgacgtc tccctcgcca tgatgcagac gctgagcgct ggcaaccagt
      421 ttgacgaagc cgagaagctg gccaaaacgg cgattgaaaa gaacaaggaa tattctcagc
      481 tctacgacgg cctctatctg gaatacctgc gccggaaccg gcaggcggag gccgaggaaa
      541 tcctgaagct caagattcgg aacaatccca cgtcgacgga atcccgtgtg cagctagccg
      601 gcttttacgc ggctacgaac cggcggcagc agatgtcgga aacgctggat tcggtgatcg
      661 agaaccgcaa gacccttccg aacgcgcttt ccatcgtagg cgatttttat taccggatcg
      721 gcgaattcga tcaggccttg aagctctatc agcatggttc cgaatcggaa cccgcccaga
      781 aactgacttt ccagaagcgc atgatcgagg tggcgctccg gcaaggcaaa cgcgaggagg
      841 cgtccaggat catcgatgaa attctcgcgg agcgtccgaa cgagccggac acggtcgcca
      901 tacgcgcggc gatgacgctc gagaccggta accaggacca gatccgcgaa gccatcaagg
      961 acatggaatc cgtggtgaaa tcgattcccc ggaacgccat tctgcggttc aatcttgggc
     1021 gcgcctatct tgcaacggga caaacggacc gggcacgcca gcaactcgaa gatgcggtca
     1081 agatggcgcc cgcttttctg ccgggcaaac tcattcttgc ggagctctat gttcgtgtcc
     1141 gcaactatgc gacagcgcgc cagctcgccg acgaggtgct cgccgtaaac gcgaacagca
     1201 cgccggcgcg gctcatccgt tccgccactc tgatggcctc gggcgaccgc gagcgggcgc
     1261 ggacggagct tctggacacg ctgaaacggc agcctcaatc ggtggaagct cagttccagc
     1321 tcggcgtgct caactaccag gaaggccgtc tcaaagacgc cgagacaagt ttcacacacg
     1381 tcaggcagca ggcgcccgat aacccccgcg cgacgctggc actcgcggaa acctatctct
     1441 cgcaggggcg tccacagcaa gccacggagc tgttgaccgc cgaagtgtcc aagcatcccg
     1501 aggcgcgcga cttgcgcatt gcacgcgcga atattgcggc cgtccggggc gagtacgaca
     1561 cggctattgc cgaaatgggc gagctcgtga agcagtatcc ggagtccgcc gatctgtatt
     1621 cccggctcgg cgaatattac cggcgaaagg gcgataccgc atccgctctc caaaacctgc
     1681 gccgcgcgcg cgagttgatg cccaacaacg catccaccaa tctgcagctt gccctgatgc
     1741 tcgaagcggc cggccggcag agtgaggcga tgccgctcta cgagcaggtg attcgtatcg
     1801 aatcggataa tcccattgcg ctgaataatc tcgcgtatat catcgccgag tccggaggtg
     1861 acctcgacag ggccctgacc tatgcgcagc gcgcgcggca gcggctgccg cagaatgccg
     1921 acgtggccga tacgctggcg ctgatctata tcaagaagaa tctcaatgat aatgccattc
     1981 agattcttag cggtcttgtc gccaagaacc cgcagcgcgc cgactaccgg tatcattacg
     2041 cgacggcgct ggcgcagaag ggcgacaagt taagtgcaaa gcgcgagttg gaagctgcgc
     2101 tccgcagcag tccaaaccag cagcaggaaa gcaagatcaa ggaactgatt gccaagttgg
     2161 gatgattccg gccagtgatc gaaccgcacc gaggtctcca aatacctgaa cggccggaca
     2221 gcccccgcaa ggaaaagcca tcggcatcgc ggcatccttc tttgccgtac gtattgccgt
     2281 tcgccgcctt tctggtgctg ctggttggac accagtatct ggaactggga agctggcagt
     2341 acccggcaag ggtcgccatc atagccctgt tactgattgt cttttcgcgc cacgtgcttg
     2401 atttccgcct gtcgcagccg gtggcgagcc tggggatcgg cgtggccgtt ttcgtaatct
     2461 ggattttgcc tgatttattg tggtccggct accgtgatca ctggcttttt cagaacggga
     2521 taaccggcac aatcaccagc tccatcgcgg accaccatcg catggatgga tgggtgatct
     2581 tcttccgcag tgtcctcgct gtcatcattg ttccggtggt tgaggagcta ttctggcgcg
     2641 cgtggctgat gcgatggctc atcaagccgc agtttgaaac cttgaagctg ggtgcctact
     2701 cggccatggc gttctgggta acggcgctgc tgtttgcttc cgagcacggg ccgtactggg
     2761 acgtgggact gctggcgggc attatttata actggtggat ggtccgcacg cggagcctcg
     2821 gagattgcat tctggcccat gcgtgcacga atgcctgctt gtccttttat gtgatcttcg
     2881 ccggtaaatg ggaatactgg ctctgagtta cggtattcgg gcgacacctc cgcggccgtt
     2941 gatcacgacc cgctcggaga cttcgcccgc catgatgaga aacacgttcg agccggcgcc
     3001 cagtttcacg tccgccgaat aagctcctgg tatttcgtca tcggcggaga tgaggttgct
     3061 gagttcgccg ccgccactct cggcaatcac tttcggatcc gccgccgcca tcccgatgcc
     3121 cgatttgtcc attacctgaa aggtgattgc attccgcaga atgtcacccg cccgcccgct
     3181 tgatgtcgaa ttcagaacgc ggatgtaaac gggtgcactg gacggcaccg cataccagta
     3241 ggggacgcgg atctccacgc cgctgccgcg cacgagcagg aacccttcgt actcgcccgg
     3301 ctccaacgcc gctccggtca actcgaccgc aacggtgcgt gtggctcccg gttcgagagg
     3361 aaaggaattc tccgaaacct gtgccgttgg agcagccccc gcggactcca ccgagatcga
     3421 aaaaacgtcg gctgcggcac cgacgttcgt gaaagaaata ttgcggcgca gtggttccgt
     3481 tccgccgctg gcgccgaagc tgatcgagac cggtgaagcg gtgaccgtcg cccgtatggc
     3541 ggcgctcagg tccagcaaac cggttcccac cttttgcgta
//
LOCUS       DASTMK010000580         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_125787, whole genome shotgun sequence.
ACCESSION   DASTMK010000580 DASTMK010000000
VERSION     DASTMK010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125787"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="VFQ79_23700"
     CDS             complement(<1..1476)
                     /locus_tag="VFQ79_23700"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0142748.1"
                     /translation="MKSNGNGSLVWIKDETRPELRGWEQFYRNRWQHDKIIRSTHGVN
                     CTGGCTWQIYVKDGIVTWEMQGLDYPALQDGIPPYEPRGCQRGISYSWYLYSPLRVKY
                     PYARGALMDLWRSARAEYDDPVEAWKSLVENPEARARWQRARGKGGFRRVDWDAAVEL
                     IAAANIYTIQKYGPDRIAGFSPIPAMSMISYASGARMLQLMGGVSLSFYDWYCDLPPA
                     SPEVWGEQTDVQESADWYNAKMLAVMGSNLNMTRTPDCHFAAEARHNGSKMWVFAPDF
                     NQVAKYADEWVAINAGQDGAWWMAVNHVLLKEFHHEQATPYFLEYTSRYTDAPFLVEL
                     KEDAGTFRAGQLLRANRLDGYADVENGDWKFLMWDANDARPKMPLGSVGHRWGQAQGK
                     WNLLLRDGVDDSEISPVLTFLNSGDGVEQVEFDDFGEARSIRRGVPVRRIRTASGEVV
                     TVATVYDLMMAQFGVPRGLEGAYPESYDDEQAPYTPAWSEKY"
     gene            complement(1473..2333)
                     /locus_tag="VFQ79_23705"
     CDS             complement(1473..2333)
                     /locus_tag="VFQ79_23705"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0142749.1"
                     /translation="MKGLSLVHFKRIRPYGILLLLLPAIAAAQDTADFFRQNCVSCHT
                     IGGGRLTGPDLKDVLTRKDRAWLAQFMLNPQAMIDRGDAYALQLQQEARGVVMPVIAG
                     LDKARAESLLTLIEVESKLEKSQFAGLQMSDRPFTAADVELGRKLFRGDQRLTNGGPA
                     CLSCHTMRSVGSLGGGRLGPDLTRVYGRLEGRKNLGAWLFAPATPTMQPIFRKHALTT
                     EEILPLVAYFESAAKAGGEDDSASGLTFLLFGLGGTAAGLVLFDAAWKKRFRAVRRPL
                     VSNNSAGEQQ"
     gene            complement(2347..2763)
                     /locus_tag="VFQ79_23710"
     CDS             complement(2347..2763)
                     /locus_tag="VFQ79_23710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU0142750.1"
                     /translation="MLSVTARYALRALTHLAAIPEGESLLGKDLAVRAEIPANYLSKI
                     LWTLGSARIIDATRGSGGGYRLHKRPEEIHLVEVVDLFDKPRVRQGCFLGQDRECSDS
                     NPCSAHEQWREVGEAYARFLESTTLATIAHFSTPVK"
     gene            2938..3438
                     /locus_tag="VFQ79_23715"
     CDS             2938..3438
                     /locus_tag="VFQ79_23715"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0142751.1"
                     /translation="MNLAEAVTRCYQCGKCTAGCPQAERMDLTPNQILRLVQLGLSER
                     AARADAIWKCVSCLTCSTRCPQEVDCAGVMDQLRQMAAAGDHRTLLFQRAFLRNVRRH
                     GRLNELELTALFKTRAMLAGAPLSFLFQEAPLAPRMYAKGKLRWKGAAVDDTTVVERI
                     FARCGE"
     gene            3441..4274
                     /locus_tag="VFQ79_23720"
     CDS             3441..4274
                     /locus_tag="VFQ79_23720"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEU0142752.1"
                     /translation="MALAYYPGCALHGTSNDYEQSVRACLRALGTPLEELRDWICCGA
                     TAAHSLNPKLATALPARNLGIAERDGYEELLAPCPMCSMELMKAAHEIGASEAVRTQI
                     SDIVELPVSGRTRVLNLIQVFQGFEIHPVRKLEGIVVACYYGCLLLRPPGIVKFDDPE
                     QPSSMEKIVNALGAKTVEWGAKTECCGAGLTLADEDSVVELAHKILSNAATHGANCVV
                     VACPMCHVNLDMKQEAIERKKGMRHNLPVYYLSDLVGLALGLDESELGIDRHFTGRVH
                     V"
     gene            4267..6219
                     /locus_tag="VFQ79_23725"
     CDS             4267..6219
                     /locus_tag="VFQ79_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HEU0142753.1"
                     /translation="MSRIGVFTCWCGENIARTVDVAAVAKAIAQMPGVRCSIDYKYMC
                     SDPGQAQIREKIESEKLTGVVVAACSPHMHLKTFRRAASTAGLNPYLVEMANIREHCS
                     WVHHDRAEATRKAIDIIRMSVEKVRHNRPLEPIRVPVHRRALVIGGGVAGIQAALDIA
                     DAGYEVVMVEREPSIGGKMAGLSETFPTLDCSQCILTPRMVEVGQHPRIRLYTYSEVE
                     TVDGFIGNFRVGIRKKARYVDIDKCNGCGDCWSVCPLKRIPSEFDYGMGFRRAIYVPF
                     PQAVPARPVIDRNTCAKLIRNGCGLCAKKCQRGAIRFDDQDEIVEEQVGAVVMATGYS
                     LWKNGYGEYGYGKFKDVIDGLQFERLVSASGPTNGELKRPSDGREPKTVVFLSCVGSR
                     DAAKGFSYCSKICCMYNAKHAMLYRHKVHDGEAHVFYMDIRAGGKGYDEFVRRAIEQD
                     GAHYHRGRVSKITEENGKLIVRGADTLAGVPLTVEADMVVLASAMRPAPGVEALAQKL
                     NAGCDEHGFLLESHPKLRPVETNAAGVFICGAAHGPKDIPESVAQASAAAAKVSVLFS
                     REELNREPEIARIDERTCAACFACLEACPYHAIEKVQLRHKMTARVNSGLCMGCGTCV
                     AVCRSKSADIDGYSEEQVYSMVEALA"
     gene            6216..6602
                     /locus_tag="VFQ79_23730"
                     /pseudo
     CDS             6216..6602
                     /locus_tag="VFQ79_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
BASE COUNT         1307 a         2063 c         2171 g         1284 t
ORIGIN      
        1 atacttttcc gaccacgccg gcgtgtaggg agcctgttcg tcgtcgtagc tttccgggta
       61 agcgccttcg aggccgcgtg ggactccgaa ctgcgccatc atcaggtcgt agaccgtggc
      121 gacggtaacc acctcgcccg aggcggttcg gatgcggcgc acggggacgc cgcgccggat
      181 ggaacgggct tcgccgaaat cgtcaaactc cacctgctcg acaccgtcgc cgctgttcag
      241 gaacgtgagt accgggctga tctcgctgtc gtcgacgcca tcccgcagca ggaggttcca
      301 cttcccctgc gcctggcccc agcggtggcc gacgctgccg aggggcattt tgggccgggc
      361 gtcgttcgcg tcccacatca gaaatttcca gtcgccgttc tctacatcgg cgtacccgtc
      421 aaggcggttt gcgcgaagca actggccggc gcggaacgta ccggcgtctt ccttcagctc
      481 caccaggaag ggcgcgtcgg tgtagcgtga ggtgtattcg aggaaatagg gcgttgcctg
      541 ctcgtgatgg aattccttca gcagcacatg gttcaccgcc atccaccagg cgccgtcctg
      601 ccccgcgttg atagccaccc actcgtcggc gtactttgcc acctggttga agtcgggcgc
      661 gaacacccac atcttggaac cgttgtgacg cgcttccgcg gcaaagtggc aatccggcgt
      721 gcgcgtcatg ttcaggttcg aacccatcac ggcgagcatc ttggcgttgt accagtcggc
      781 ggattcctgc acgtcggtct gctcgcccca gacttccggg gaggccggcg gcaggtcgca
      841 ataccagtcg tagaaggaaa gggaaacgcc gcccattaac tggagcatgc gggcgccgga
      901 agcgtagctg atcatcgaca tggccgggat gggcgaaaac cccgcgatgc ggtccgggcc
      961 gtatttttgg atggtgtaga tgttggcggc ggcgatgagt tcgacggcgg cgtcccaatc
     1021 cacgcggcgg aatccgcctt taccgcgggc gcgctgccag cgtgcgcgag cctcgggatt
     1081 ctctaccaga gatttccagg cctctacggg atcgtcgtat tcggcgcgcg cgctgcgcca
     1141 cagatccatt agagcgccgc gagcataagg atacttcacc ctcagcgggc tgtacagata
     1201 ccacgagtac gagatgccgc gctggcatcc acgcggctcg taaggcggaa tgccgtcctg
     1261 aagcgcgggg taatcgaggc cctgcatctc ccaggtaacg atgccgtcct ttacataaat
     1321 ctgccacgtg cagccgcccg tgcagttcac tccgtgagtg ctgcgtatga tcttgtcgtg
     1381 ctgccagcgg ttgcggtaaa actgctccca cccgcgcagt tcggggcgtg tctcgtcctt
     1441 gatccacacc agactgccat tgccgttcga cttcattgct gctctcctgc cgagttgttg
     1501 gaaaccaggg gacgccgcac cgcgcggaaa cgctttttcc atgccgcgtc gaacaaaacc
     1561 aggcccgcgg cggttccgcc caggccgaat aacaggaacg tgagaccgga tgcggaatcg
     1621 tcttccccgc ccgccttcgc ggcactttcg aaataagcca ccagcgggag aatctcttcg
     1681 gtcgttaacg catgcttccg gaagatgggc tgcatggtcg gcgtcgctgg cgcgaacagc
     1741 cacgcgccca ggtttttgcg cccttcgaga cgaccataga cgcgggtgag gtcgggcccg
     1801 agccggcccc cgccgagcga acccacgctc cgcatggtgt ggcaggacag gcaggcgggt
     1861 ccgccgtttg taagccgctg atctccccgg aagagcttgc ggcccagctc gacgtccgcc
     1921 gcggtgaacg gacggtcgct catctggagg cccgcgaact gggacttttc cagcttcgat
     1981 tcgacctcga tgagcgtcaa cagcgattcg gcgcgggctt tatcgagccc cgcaatcacg
     2041 ggcatcacca cgcctcgcgc ctcctgctga agctgaagcg cgtaggcatc gccgcggtcg
     2101 atcatggcct gcggattcag catgaactgc gccagccagg cacggtcctt gcgggtgagc
     2161 acgtccttca ggtccggccc ggtgagccgg ccgcccccga tggtgtggca gcttacgcaa
     2221 ttctgccgga agaaatccgc cgtatcctgc gcggccgcta tcgcaggcag caggagcagc
     2281 aggataccat acggccttat cctcttaaaa tgaaccaaag aaagcccttt catttggaac
     2341 tcccagttat ttaaccggcg tgctgaaatg agcgatggtg gccagcgttg tcgattccag
     2401 gaatcgagcg tacgcttcgc cgacttcccg ccactgttca tgcgcggagc agggattgga
     2461 atccgagcat tcccggtcct ggcctaaaaa gcacccctgc cgcacgcgag gcttgtcaaa
     2521 aagatcgacg acctcaacca ggtgaatctc ttcgggacgc ttatgcaggc gatagcctcc
     2581 gccgctcccc ctggtcgcgt cgataattcg ggcgctgccc aacgtccaaa gaattttgga
     2641 gaggtaattc gccggaatct ccgctcgcac tgccagatct ttgcccaaca gcgactctcc
     2701 ctcgggaatg gcagcgaggt gcgtcaaagc tctcagagcg tatcttgcag taacggacag
     2761 catcagttgt tcacttcttc atgaagaagt atacaactca ggaaacggtt gtcaagtagg
     2821 aattgtaaac aataggaaaa aagctatcct gctcaataca cgaccattta agtccaaatt
     2881 gaagttatgg ggctcaaagt atcaaatatt aaggtctgaa tatcgatata ataaaaagtg
     2941 aatctcgcag aagcagttac gcgctgttat cagtgcggca agtgcacggc gggttgcccg
     3001 caggcggaac ggatggatct gacacccaac cagatccttc gcctggtcca gcttggtctt
     3061 tccgaacggg cggcgcgcgc ggacgcgatc tggaaatgcg tctcctgtct cacctgctcc
     3121 acgcgctgtc cgcaggaagt cgattgtgcg ggcgtcatgg atcaactccg gcagatggct
     3181 gcggccgggg accatcgcac cctcctcttc cagcgcgctt ttttgcgcaa tgtccgccgc
     3241 catgggcggc ttaacgagct ggagctgacg gcactcttta aaacccgcgc gatgctggcg
     3301 ggtgctccgc tcagctttct gtttcaggag gcgcctctgg cgccgcggat gtacgccaag
     3361 ggcaagctgc gctggaaagg cgccgcggtg gacgatacca cggtagtgga gcgcatcttt
     3421 gcaaggtgcg gagagtagcc atggccctgg catactatcc cggctgcgcg ctgcacggaa
     3481 cttcgaacga ttacgaacag agtgtgcggg cgtgcctgcg cgcgctgggc acgccgctcg
     3541 aagaactgcg cgactggatc tgctgcggcg ccacggcggc gcattcgctc aatcccaagc
     3601 tcgctaccgc gctgcccgcg cgcaacctgg gcatcgccga gcgcgacggg tacgaagaac
     3661 tgctggcgcc gtgccccatg tgttcgatgg aactcatgaa ggcggcgcac gagatcggcg
     3721 cgagcgaggc ggtgcgaacg cagatctccg atatcgtcga actacccgtc tcgggtcgca
     3781 cccgcgtgct gaacctgatc caggtttttc agggattcga gatccatccg gtccgcaagc
     3841 tcgaaggcat cgtagtggcg tgctattacg ggtgcctgct cctgcgtcct cccggcatcg
     3901 tgaagttcga cgatccggaa cagccttcca gtatggagaa gatcgtcaac gcgctcggcg
     3961 cgaagacggt cgagtgggga gcaaagaccg aatgctgcgg cgcgggcctt acgctcgccg
     4021 atgaagacag cgtggtggaa ctggcgcaca agattctgtc gaatgcggcg acgcacggcg
     4081 cgaactgcgt cgtcgtggcc tgccccatgt gccacgtgaa cctcgacatg aagcaggagg
     4141 ccatcgagcg gaagaaaggc atgcgccaca acctgccggt ctactatctc tccgacctgg
     4201 tgggactggc cctggggttg gacgaaagcg aactcggcat cgaccgccac ttcaccggga
     4261 gagttcatgt ctagaatcgg cgtgttcacc tgctggtgcg gagagaacat cgcccgcacg
     4321 gtggatgtag cggcggtcgc gaaggccatc gcccaaatgc ccggcgtgcg ctgctccatc
     4381 gactacaagt acatgtgctc ggacccgggg caggcgcaga tccgtgagaa gatcgagtcc
     4441 gaaaaactga cgggcgttgt cgtcgcggcc tgttcgccgc acatgcacct gaagaccttc
     4501 cggcgggccg cgtccaccgc cgggctgaat ccttacctgg tggagatggc gaatatccgc
     4561 gagcattgct cgtgggtaca ccatgaccgc gcggaagcca cacgcaaggc gatcgatatt
     4621 atccgcatga gcgtggagaa ggtccggcac aaccgcccgc tcgagccgat acgcgttccg
     4681 gtgcatcggc gcgcgctggt gatcggcggc ggcgtggccg ggattcaggc cgcgctcgat
     4741 atcgccgacg cgggctacga agtggtgatg gtggagaggg agccttcgat cggcggcaag
     4801 atggcggggc tgtcggagac gttccccacg ctcgattgct cgcaatgcat tctgacgccg
     4861 cgcatggtgg aggtggggca gcatccgcgc atccggctct acacctactc ggaagtggag
     4921 acggtggacg gtttcatcgg caacttccgc gtggggatcc gcaagaaggc gcgctatgtc
     4981 gacatcgaca agtgcaacgg ttgcggagat tgctggagcg tctgtccgct caagcgaatc
     5041 cccagcgaat tcgactatgg catgggtttc cggcgcgcga tttacgtgcc gtttccgcaa
     5101 gcggtgccgg cgcgcccggt gatcgaccgc aacacctgcg ccaagctgat ccgcaacggc
     5161 tgcgggctgt gcgcgaagaa gtgccagcgc ggcgcaatcc ggttcgacga tcaggatgaa
     5221 attgtcgaag aacaggtggg cgcggtggtg atggccaccg gatactcgct ttggaaaaac
     5281 ggctatggcg agtacgggta cggcaaattc aaagacgtaa tcgacgggct gcaattcgag
     5341 cgcctggtct ccgcttccgg cccgaccaac ggcgagttga aacgtcctag cgacgggcgc
     5401 gaacccaaaa ccgttgtgtt cctcagttgc gtgggctcgc gcgatgcggc caaaggcttc
     5461 agctactgct cgaagatctg ctgcatgtac aacgccaagc atgccatgct gtaccgccac
     5521 aaggtgcacg atggggaagc gcacgtgttc tacatggaca tacgcgccgg cggaaaaggg
     5581 tacgacgagt tcgtgcggcg cgccattgaa caggatggcg cgcattacca ccgcgggcgt
     5641 gtttcgaaaa tcacagagga gaacggcaag ctgatcgtcc gcggcgccga cacgctggcc
     5701 ggcgttccgc tcacggtgga ggccgacatg gtggtgctgg cttccgcgat gcggcccgct
     5761 cccggtgtgg aagcactggc gcaaaagctg aacgccggat gcgacgagca cggctttctg
     5821 ctcgaatcgc atcccaagtt gcggccggtg gaaacgaatg ccgcgggtgt ttttatttgt
     5881 ggcgcggcgc acggccccaa agacattccg gaatccgtgg cgcaggcgtc ggcggcggca
     5941 gcgaaagttt ccgttctgtt ttcgagagag gaactgaacc gagagccgga gattgctcgc
     6001 atcgacgaac ggacctgtgc cgcctgtttc gcgtgtcttg aagcctgccc gtatcacgcc
     6061 atcgagaaag tacagttgcg gcacaagatg acggcgcgcg tcaattccgg gctgtgcatg
     6121 ggatgcggca cgtgtgtggc ggtgtgccgg tccaagagtg cggatatcga cgggtactcc
     6181 gaagagcagg tctattcgat ggtggaggcg ctggcatgag tgaaccaaaa atcgtcgcct
     6241 atgtctgcaa ctggtgcact tatctcggtg ccgatctggc gggcacaaac cgcctggaat
     6301 acccgcccaa tgtacgcctg gtccgtctgc cctgcaccgg acggatcgac ttcaacctga
     6361 tcgtgaaggc gttcgagatt ggcgccgatg cgattctggt ttccggatgt catcccggcg
     6421 attgccacta caccgcgggc aactaccacg cccggcggcg ctggatgctg ttccggccgc
     6481 tgctcgatac gctcggcttc gatctccgcc gcatccactt cacttggatt tcggccgcag
     6541 aaggcaggaa gttccaacag gtgatctcgg agataacgga atccgcgcgg gcgctgggtc
     6601 cggccgatta tctgaaacgg cgcgaagcag ctccggttgt gccgcgcacc gtgcctgcgc
     6661 cggttccgcc ggttgtggat gaagccgggt tgcgggcaaa cgccgccggc ctgctgcggt
     6721 cgggggaagc acaagtggtg atcggctatg acgcggcggg aactccgatg ttcgtgcggc
     6781 gcgaggagga ttgttcgcgg ctggcggtca acgggaatgc gcgcg
//
LOCUS       DASTMK010000581         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_125792, whole genome shotgun sequence.
ACCESSION   DASTMK010000581 DASTMK010000000
VERSION     DASTMK010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125792"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFQ79_23735"
     CDS             complement(<1..744)
                     /locus_tag="VFQ79_23735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0142754.1"
                     /translation="MDASRWERIQSLFHKVSDLQDPREQAAILRAECGDDGSLIDEVL
                     AMLAADRKASGLLDASVGHLAEQVLGDPVIPPAAYKEIGPYRIQRVLGEGGMGTVFLA
                     GRADLGTLAAIKILRDAWISPARRERFAVEQRTLAHLDHPNIARLLDANTLADGTPFF
                     IMEYVDGVPIDKYCDTHGGPIIERIRLFRSVCEAVQFAHRHAVIHRDLKPSNILVKSD
                     GTVKLLDFGISKHLDNLEIPLDATRTGLRL"
     gene            complement(734..1336)
                     /locus_tag="VFQ79_23740"
     CDS             complement(734..1336)
                     /locus_tag="VFQ79_23740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU0142755.1"
                     /translation="MPVDFFLTGDRGGSAGSKPWPKNREALDHLFSVTYEELRRLASA
                     VRRRDPAATLSPTTLVNEAWLKLAKTPAVAQSSHLHFKRIAARAMRQVLIAAVRRRTA
                     YKRDAGPGMLVAFDDAMGPVTSCDEELIALDAALDELARMNPRHAAMVESRFFGGLEV
                     NEIAALLGVSEATILRDWRAARAWLARQLGSASMERTDGR"
     gene            1519..1836
                     /locus_tag="VFQ79_23745"
     CDS             1519..1836
                     /locus_tag="VFQ79_23745"
                     /inference="COORDINATES: protein motif:HMM:NF036614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HEU0142756.1"
                     /translation="MPVAKIEIQQVLGALKATGSSDPDVLFARKEELLAESRKMKLLG
                     VFPIVIGVVMSLTVIGAVVGIPAIFFGLFVRKRIRTNVQNAESAYTEYLASIGVQAKA
                     ATA"
     gene            1849..>3374
                     /locus_tag="VFQ79_23750"
     CDS             1849..>3374
                     /locus_tag="VFQ79_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142757.1"
                     /translation="MRRTQSFFAALFALVFPAAAAPAQKPAPAQQSEWLAVCSRCPSP
                     AVVSKSGIGTANARAEARMNREELKGWCENESPGSVDACLREQLAENGNKVYRASADC
                     TAGRITTIEEKTYTLAGLWDNSDIGGGRTKWRDAGGQIVGRDNASNGLAISQQWEVLC
                     PGPPPVGRAAAASAQAPRAATPARQAAQPPPAPVCGGQPMCTEVNSFAATITDFRTSN
                     VSGAKVVTASVRFQNKLARPIILGYVAGSGVTTDDQGNRYTVYAADSVRGIGAIASGR
                     VDPKFILRAGEAGDARFEMVWRWSGREVFGLAFEMELTVRELLPLPSGQFRMGPEHPL
                     RFSGLGNAAVSTAPAVPSTPATSAAPVAAVAATPAAGDAPAVSAAPAAATAEADPCAG
                     AARCFSAGPFVAEVQQISSSLSGGRHHVLRFNMRFRNLTAQPLILAATYNSSIAIDND
                     GNRYGVTPSQAVSGMWVTTGRNADPQFVLAPGEFRNAAFQVYRRNSGNPTGLSYTFDV
                     "
BASE COUNT          643 a         1076 c         1020 g          635 t
ORIGIN      
        1 cagccggagt cccgtccttg tggcgtccaa cgggatttcc aggttgtcca ggtgcttgga
       61 aataccaaag tcgaggagct tcactgtgcc gtcgctcttc acaagaatgt tggaaggctt
      121 caggtcccgg tgaatcaccg cgtgccggtg cgcgaactgt accgcttcgc acaccgaccg
      181 gaataaccgg atccgctcga taatcggacc tccgtgcgta tcgcagtact tgtcaatcgg
      241 tacgccgtcg acgtactcca tgatgaaaaa aggcgttccg tcggcgaggg tattcgcatc
      301 gagcaatcgt gcgatgttcg gatgatcgag atgggcgagc gtacgctgct ccacggcgaa
      361 gcgctcccgg cgcgcggggg agatccatgc gtcgcgaagg atcttgatgg ccgcgagagt
      421 tccgaggtcg gcccgcccgg caaggaagac cgtccccatg ccgccttctc ctaacacgcg
      481 ttggatccgg tacggaccta tttctttata ggcggcggga ggaatgaccg ggtctcccag
      541 tacctgctcc gcaagatgac ccacgctggc atcgagcagc ccggacgctt tccggtccgc
      601 tgccagcatc gccagcactt cgtctataag ggacccatca tcgccgcatt cggctctcag
      661 aatggccgcc tgctcgcgcg ggtcctgaag atccgagacc ttatggaaca gggattggat
      721 tcgctcccac cggctagcgt ccatcggtcc tttccatgga tgcggaaccg agctgccgtg
      781 caagccaggc tcttgccgcg cgccagtcgc ggaggatcgt cgcttctgaa acaccaagaa
      841 gagcggcgat ctcgtttacc tcgaggcctc cgaaaaagcg gctctccacc atcgccgcgt
      901 gacgcggatt catgcgggcc aactcgtcga gagcggcgtc gagcgcaatc agttcttcgt
      961 cgcacgatgt gaccggtccc atggcatcgt caaatgccac gagcataccg ggaccggcgt
     1021 cgcgcttata ggcggtcctc cgccggacgg cggcgatcag gacctggcgc atcgcacggg
     1081 cggcaatccg cttgaaatgg agatgggacg actgcgcgac cgccggcgtc ttcgccagct
     1141 tcaaccatgc ttcgttaacc agggtggtcg ggctcagcgt cgcggcaggg tcccgccgcc
     1201 ggacggccga agcgagccgg cgcaattctt cataggtgac gctgaaaaga tgatcgagcg
     1261 cttccctgtt ttttggccat ggcttagagc ccgctgaacc accccggtct cccgttagaa
     1321 aaaagtcaac cggcatgaca gttctcgccc cccacttgcg taatgtctga gtgcaaccgc
     1381 taccctcggc gcgatcaggg attgttatcg cgaagatagc atccaatgcc ggagccgcac
     1441 aactgcgaat cggaataagt acgccggcaa agtactgggc cgcccgggta cggaatactt
     1501 cagttatagg agcgaaacat gccagtcgca aagattgaga tccaacaggt cctgggcgcc
     1561 ttgaaggcga ccggctcgtc cgatccggac gtactcttcg cacgcaaaga ggagttactt
     1621 gccgaatcca ggaagatgaa gctgcttggt gtttttccca ttgtcatagg tgtggtgatg
     1681 tctctcaccg taatcggggc tgtggtcggc attccggcca tcttcttcgg gctctttgtg
     1741 aggaagcgca tccgcacaaa cgttcagaac gccgagtcgg cctataccga gtacctggct
     1801 tccatcgggg ttcaggccaa ggccgccacg gcttaaagga aagacgccat gcgacgaacc
     1861 cagtccttct ttgccgctct gtttgcgctg gtctttccgg ctgccgccgc acccgcgcag
     1921 aaacccgctc ccgcccaaca gtccgaatgg ctcgcagtgt gttcccgatg cccgagtccg
     1981 gctgtggtat cgaaatcggg gatcggcacc gcgaacgcca gggccgaggc acgcatgaac
     2041 cgtgaagagc tcaagggctg gtgtgaaaat gagagtcctg gaagtgtcga tgcatgcttg
     2101 cgggagcaac ttgccgagaa cggtaataag gtttaccgcg cttctgccga ctgtaccgcc
     2161 ggccggatca cgacgatcga agaaaagacc tacactctgg ccggactgtg ggataacagc
     2221 gacatcggag ggggcaggac aaagtggcgt gatgccggcg gacagatcgt cggacgcgac
     2281 aacgccagta acggcctggc catctcgcaa caatgggaag tgctatgtcc cggccctcca
     2341 cctgtcggga gagccgcagc cgcttccgcc caggcgcctc gggctgccac acccgcccgg
     2401 caagcggccc agccgccgcc tgcaccggtg tgcggcggac aaccgatgtg caccgaggtg
     2461 aattcgtttg cggcgacgat taccgatttc cgcaccagca acgtctccgg cgccaaggtg
     2521 gttacggcct ccgtgcgttt ccagaacaag ctcgcgcgcc ccatcatcct gggctacgtg
     2581 gccggctccg gcgtcacgac cgacgatcag ggtaaccgct acaccgtcta cgcggcggac
     2641 agcgtgcgcg gcatcggagc tatcgccagt ggcagggtgg atcctaaatt tatcctccgt
     2701 gccggtgaag ccggagacgc ccgctttgaa atggtctggc gctggtccgg gagggaggtc
     2761 ttcggcctgg catttgaaat ggagttgacc gttcgtgagc tcctccctct gccaagcggc
     2821 cagttccgga tgggcccgga gcatccgctg cgttttagcg ggctgggcaa cgccgccgta
     2881 tctaccgcgc cggctgttcc ctccacgcca gctacatccg ctgcaccggt tgccgctgtt
     2941 gccgccactc cagccgctgg tgacgctccc gccgtcagtg ccgcgccggc ggccgccact
     3001 gccgaagccg acccgtgcgc cggcgccgcc cgctgcttct ctgccggtcc gttcgtcgcg
     3061 gaagtgcagc aaatatcgag cagcctctcg ggtggcaggc atcacgtcct gcgcttcaat
     3121 atgcgtttcc gcaacctgac cgctcagccg ctgattctgg cggccaccta taattcctcc
     3181 atcgccatcg acaacgacgg caaccggtac ggtgtcacac ctagccaggc tgtcagtggc
     3241 atgtgggtga ccaccggcag aaacgccgac ccgcaattcg tcctcgctcc cggagaattc
     3301 cggaatgcgg cgtttcaggt gtaccgccgg aacagcggca atccaaccgg actgtcgtat
     3361 acgttcgacg tcag
//
LOCUS       DASTMK010000582         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_476993, whole genome shotgun sequence.
ACCESSION   DASTMK010000582 DASTMK010000000
VERSION     DASTMK010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476993"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1164)
                     /locus_tag="VFQ79_23755"
     CDS             complement(61..1164)
                     /locus_tag="VFQ79_23755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0142758.1"
                     /translation="MRKSLVLAAAAVALLQAEPLTVKHVVVYREAGRFAGWPANHGAW
                     SWGDEILVGFEAGQFRKTDQGHAIDYSKPAEHVLARSLDGGESWSVERPEGLRPPPGE
                     KIAGVPTPEGGRPLTDCPGGVDFSHPDFALTARMTSIHDGQSRFYYSMDRGRNWQGPF
                     RLPNFGQPGTAARTDYLVNGKHDLTMFLTAAKSNGREGRVICVRTRDGGKTWNFESFV
                     TQEPEGNEYAIMPSSIRLGRDTILTAVRYRKFIDAYRSSDNGKTWAYAGRPAPETGGN
                     PPSMISLRDGRLALTYGYRLKPYGVRARLSADNGKTWGEELVLRQDGGSWDLGYPRTV
                     QRRDGKLVTVYYFNTGADSERFIGATIWDPGSR"
     gene            1221..2411
                     /locus_tag="VFQ79_23760"
     CDS             1221..2411
                     /locus_tag="VFQ79_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEU0142759.1"
                     /translation="MRVLLFSGKGGVGKTSLAAATGLRLGELGYRTLIMSVDPAHSLG
                     DSFDLKGSLFHSETADPLAIHERLFIQEVNIQREIRRHWREISAYMISVLRTTGLTDL
                     EAEELAILPGMEELSAMMYVNQYRRENRYDVIVLDAAPTAESMRFVSMPTTLDWYMKH
                     VFPVHRNVLKAVRPIANRVSPIELPGDSYFANMRELFQKLDGIADVLEDPNLTSVRLV
                     TNPEKMVLRETQRAFVYFSLHGLTVDGIIINRVLPESVNDTFFAEWRRAQQQVLHEIE
                     EYFAPVPTRRVPLFTNEVVGISRLQELARTLYADGEDPAAVVQTEKPYSFCKQANHYE
                     VRLNVPFATKGEIGLFKKGDELVVEIGTLRRHIGLPTSMAGLQPARARLENRVLIVEM
                     KEVA"
     gene            2408..2659
                     /locus_tag="VFQ79_23765"
     CDS             2408..2659
                     /locus_tag="VFQ79_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142760.1"
                     /translation="MNDAQGSPGETAYDARKHRENCVFCAAGEAISGMLKGMGPSENV
                     RVHFRNARVEILKGFRAMLDQRIERVARRGEKGTPVVVE"
     gene            2672..3136
                     /gene="bcp"
                     /locus_tag="VFQ79_23770"
     CDS             2672..3136
                     /gene="bcp"
                     /locus_tag="VFQ79_23770"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEU0142761.1"
                     /translation="METENLKEGVEAPEIELQTDTGEPFRLSALRGRKVVLYFYPKAD
                     TPGCTTESCEFRDSMPAIAEKDAVVIGVSPDKPQAQAKFKNKYKLPFTLLADVDHRAA
                     DAYGVWKEKSMYGKKYMGIERTTFLIGEDGKIRKIFSKVKPSGHAGEVLGAL"
     gene            3230..>3597
                     /locus_tag="VFQ79_23775"
     CDS             3230..>3597
                     /locus_tag="VFQ79_23775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142762.1"
                     /translation="MSASVHSPVVQPPEVRPAVPEPERKGPARWKLLAIAVVVVVLGW
                     LAYRQWVKQTAAPSAAVPAFRTSIAVQGVLQRTVRVSGQTSARNYASVTIPLLRGPDS
                     RDNLTLLTLAKSGSLVKKGDL"
BASE COUNT          749 a         1025 c         1108 g          715 t
ORIGIN      
        1 gcacagaagc agcagatcac cagccctcgg tccggacaca tgacactctg gtgagcccag
       61 tcaacgggaa ccggggtccc agatggtggc tccgatgaaa cgctcggagt ccgcgccggt
      121 attgaagtag tagaccgtta ccaacttgcc gtcgcgccgc tggacagttc gggggtagcc
      181 gagatcccag gacccgccgt cctgtctcaa gaccaactct tcaccccagg tcttgccgtt
      241 gtcggcgctg aggcgggcgc gaacaccata aggctttaac cggtatccgt atgtcagcgc
      301 caggcgtcca tcccggagtg aaatcatcga cggcgggttg cctccggttt ccggcgccgg
      361 acgtcccgcg taagcccagg tctttccgtt atcgctgctt cgatacgcat cgatgaactt
      421 tcgataccgc acggccgtca gaatggtatc ccggcccaag cggatcgacg aaggcatgat
      481 cgcatactcg ttgccctcgg gttcctgcgt aacaaacgat tcgaaattcc acgtcttgcc
      541 gccgtcgcgc gtcctgacgc agatcacccg gccttcccgg ccattggatt tcgcggcggt
      601 caggaacatc gtgagatcgt gctttccgtt cacaaggtaa tccgtccggg ccgctgtgcc
      661 gggctgtccg aagttcggca ggcggaatgg accttgccag ttcctgcccc ggtccatgga
      721 atagtagaag cgcgattggc catcgtgaat gctggtcatc cgcgcggtca gagcgaagtc
      781 aggatgggag aaatcgacac cgcccgggca atcagtgagc gggcggcccc cttcgggcgt
      841 aggcacgccg gcgattttct cacctggggg cgggcgcagt ccctccggcc tttccacgct
      901 ccaggactct cccccatcga gcgaacgcgc caaaacgtgt tcggcaggtt tggaatagtc
      961 aatcgcatgt ccctggtccg tcttacggaa ctgcccggct tcgaagccca ccagaatttc
     1021 gtcgccccag gaccaggcgc cgtggttcgc aggccagccg gcaaaccggc ccgcttcgcg
     1081 ataaacgaca acgtgcttca cggtaagggg ttcggcctgt agcaacgcaa ccgcggcggc
     1141 cgccaggaca aggcttttcc tcatactcgg attgaatcac tcattctacc cgtttagcac
     1201 atacgccgta gaatagaagt atgagggtcc tcctgttcag cggcaaagga ggtgtgggga
     1261 agacgagtct ggcggcggcg acgggtttgc gcctcggcga actcggctac cggacgctga
     1321 ttatgagcgt cgatccggct catagcctgg gagactcctt cgatttgaaa ggcagcctct
     1381 ttcattccga gacggcggat ccgctggcta tccacgagcg gctgttcatt caggaagtaa
     1441 acattcagcg cgagatccgc aggcactggc gcgagatctc cgcttacatg atttcggtat
     1501 tgcggactac cggcctgacc gacctcgaag cggaggaact ggcgattctg cccggcatgg
     1561 aagagttgag cgcgatgatg tatgtcaacc agtaccggcg cgagaaccgg tacgacgtga
     1621 tcgtgctcga tgccgctcca accgccgaat cgatgcggtt tgtgagtatg ccgacgacgc
     1681 tcgactggta catgaagcac gtatttccgg tacaccgcaa cgttctcaaa gctgtccgcc
     1741 ccatcgccaa ccgggtgtct cccatcgagt tgccgggcga tagctacttc gcgaacatgc
     1801 gggagttgtt ccagaaactg gacggcattg cggacgtgct ggaagatccg aacctgacca
     1861 gcgttcgact ggtcacgaat ccggagaaga tggttttgcg ggaaacgcag cgggcctttg
     1921 tttacttctc cctgcacggg ttgacggtgg acggcatcat catcaacagg gtattgccgg
     1981 agtcggtgaa tgacaccttc ttcgcagagt ggcgccgcgc ccagcagcag gtgctgcacg
     2041 agatcgaaga gtacttcgcg ccggtcccta cgcggcgcgt acctttattc acaaacgagg
     2101 tggtgggtat ttcccgcctg caggaactgg cgcgaaccct ctacgcggac ggggaggatc
     2161 cggccgccgt ggtccagacg gaaaaaccct acagtttctg caagcaggca aaccattacg
     2221 aggttcgtct gaacgtgccg tttgccacca agggggagat tgggttattc aagaagggcg
     2281 acgaattggt ggtcgagatc ggcacgttgc ggcggcacat cggtttaccc acgtccatgg
     2341 ccgggctaca gccggcccgg gcgcggctgg aaaacagagt tttgatcgtg gagatgaagg
     2401 aggtcgcatg aacgacgcac agggaagtcc cggcgaaacg gcttatgatg cccgaaaaca
     2461 ccgggaaaac tgcgtgtttt gcgcagccgg tgaggcgatt tcggggatgc tcaaaggcat
     2521 ggggccgtcc gagaacgtac gggtccactt tcgcaatgcc cgggtggaaa tcctcaaagg
     2581 cttccgggcc atgttggatc aacgaattga gcgcgtagcg cggcgcggcg aaaagggaac
     2641 tccggtggtg gtggaatagc cgaatcgaaa aatggaaact gaaaacctga aagagggcgt
     2701 ggaggctccg gagattgagc tccagacgga cacgggcgaa ccttttcgcc tatcagcttt
     2761 gagagggcgc aaggtggttc tttattttta cccgaaagcc gatacgcccg gttgtaccac
     2821 ggaatcctgc gaattccgcg attccatgcc cgcaattgca gaaaaggacg ccgtggtgat
     2881 aggtgtttct cccgacaagc cgcaggcgca ggccaaattc aagaacaaat ataagcttcc
     2941 gtttaccctg ctggccgatg tcgaccaccg ggccgccgac gcttacggcg tgtggaaaga
     3001 gaagagcatg tacggcaaaa agtacatggg aatcgaacgg acgacgtttc tcatcggcga
     3061 agatggcaag atccggaaaa tcttttcgaa ggtcaagcca agcggccacg ccggggaagt
     3121 tttgggcgct ctttgaagat tttggggccg gcggggcagg gggagtaacc tcgcctccgg
     3181 tttactcgcc tttcgttgag gtgattggtc tagtatcctt gaggtaaaga tgtccgcttc
     3241 cgttcactca cccgtggtgc aaccgcctga agtgcgcccg gccgttcccg agcccgaacg
     3301 caaaggtccg gcgcgatgga aactgttggc catagccgtg gttgtggtgg tgctgggatg
     3361 gctcgcgtac cggcaatggg tgaagcagac ggccgctcca agcgccgccg ttccagcgtt
     3421 tcgcacgtca atagccgttc agggagtgtt gcaaaggacg gttcgcgtga gtggtcaaac
     3481 ctcggcccgt aattacgcga gtgtcaccat tcctctgctg cggggtcccg attcccggga
     3541 taatcttact cttctgacac tggcgaagtc cggctctctg gtcaaaaagg gcgatct
//
LOCUS       DASTMK010000583        46465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_62863, whole genome shotgun sequence.
ACCESSION   DASTMK010000583 DASTMK010000000
VERSION     DASTMK010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 46465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46465
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62863"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VFQ79_23780"
     CDS             complement(<1..1302)
                     /locus_tag="VFQ79_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0142763.1"
                     /translation="MKSTLQKLTGRRSFLTAAGGALGTAFWADDTLDAATQHVNTRSK
                     PSELKITDLRVAVVARAPMTCPVIRIDTNQGISGLGEVRDGASKTYALMLKSRLLGEN
                     PCNVDKVFRKIKQFGGHARQGGGVCGVEMALWDLAGKAYNVPVYQMLGGKFRDRIRCY
                     ADTTSSHDPKVYGERMKKRMEQGFTFLKMDLGANLVEKVPGALTRPVGSTLREMNNTQ
                     HMFTGIELTDKGIGMMADYAGGIRDVIGMEVPLSADHFGHIGVNSCIRLGKALEKYNL
                     AWLEDMIPWQHTELMKQITDAVDIPILTGEDIYLKEPFFELCRKHAVDMIHPDLATSG
                     GILETKKIGDGAQEYGVAMAMHFAGTPVSFMANLHCAAATENFVALEHHSVDVPWWED
                     LVQGIEKPIVNRGFAKVPEGPGLGITLNDEVMKQHLLEPGYF"
     gene            1412..2386
                     /locus_tag="VFQ79_23785"
     CDS             1412..2386
                     /locus_tag="VFQ79_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0142764.1"
                     /translation="MRVGVFTALLSQFSLDQVIEKIKNLGIDTVELGTGNYPGDPHCK
                     LSMLDNDVELKTFKQKLSDNGISISALSCHGEPLHPNAEIRNKNRETQRKTILLAEKL
                     GVPVVIDFSGCPGDSDDSKYPNWVTSPWPPHYQELLQWQWEKQAAPFWTERTKFAADH
                     GVKIAIEMHPGFLVYSPETMLKLRAIAGPNIGCNYDPSHMFWQNIDPIAAIRVLGDSI
                     FHVHAKDTQIYHRNLPVSGVLDMKLYTDERNRAWIFRTCGYGHGAEWWREFVSTLRMF
                     GYDYALSIEHEDSLMSPNEGLTKAAAFLNDIVIREDTTAPWWVSNVSA"
     gene            2623..2979
                     /locus_tag="VFQ79_23790"
     CDS             2623..2979
                     /locus_tag="VFQ79_23790"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0142765.1"
                     /translation="MASDQRTANNKLAPQLNLALLREKKKISLRDIEDETKISIRFLK
                     AIEDEEFNQLPGGIFSTSYIRQYAAIVGHDETELIEHYRTKMGLNEPEESQGPGRKGK
                     RQQEKVSRAFAFPPFS"
     gene            2957..4459
                     /locus_tag="VFQ79_23795"
     CDS             2957..4459
                     /locus_tag="VFQ79_23795"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142766.1"
                     /translation="MRFLRFPDRAALFVLACLVGACCASGASIEGTVVENSAGKPIAR
                     TRVTLEGTGSTSGGATVLSSSSGQFAFRSLSAGTYLLRAERAGFAVTRYGQRRWNAPG
                     TPIVLDAETQHIVQIRLRKLGAVTGEIGDENQLGLPGVTVHAYRAASKLTLAGSGVTD
                     DRGIYRISNLEPGKYLIRSAARLLEDQQGLLPTFFGQTASAGEARPVIVQLEQDTTGV
                     NIQPLPGRLASLQGTVASPAAVFISLYGDTGVLRKQVPPGGEFHFDQLAPGPYSLLAQ
                     AGDRSAPLSSFQKIWIGGESQRVTVELAPSPRLILRCENRNGNPLQHKAVSIFLRRRD
                     LKDSTTDRAVCGDVLPLTPGTWEVAASAPPDIYVSGLRGARISPFAHEFDMVSGRNTE
                     VVILFSSDPGSLRGTVSTRDGAPAIGAPVYFNPVDSDLRSRLGGLRTTRTGASGKFWL
                     NGFPPGRYEVLSTFSPVDGESEDWKPGRGKLVTLGEGGDMTVEIVLEDLE"
     gene            4556..4855
                     /locus_tag="VFQ79_23800"
     CDS             4556..4855
                     /locus_tag="VFQ79_23800"
                     /inference="COORDINATES: protein motif:HMM:NF016223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEU0142767.1"
                     /translation="MKIHDPNGYGTGIHGPGGPSGADAVGRGTSARGSRQTGLRNDQV
                     EISNLAGHLSALTSDSPERLSRIEKLSADFQAGRYQVDAAVISRAMVEDGLGARS"
     gene            4855..5214
                     /locus_tag="VFQ79_23805"
     CDS             4855..5214
                     /locus_tag="VFQ79_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142768.1"
                     /translation="MSGTAALERVRRARDLVATLQSELSSGGVQALMMSPDRLERALA
                     SLEPVSSAPAVETGEQDVLLADLNAFRQDLARVRSLLVQAAGFYIGCEPSAEQEAGYG
                     PQAGLLSPDASRVMVTG"
     gene            5217..6599
                     /gene="flgK"
                     /locus_tag="VFQ79_23810"
     CDS             5217..6599
                     /gene="flgK"
                     /locus_tag="VFQ79_23810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEU0142769.1"
                     /translation="MGNFFSSLSSVAAGMRVYERALNTIQNNVSNASTPGYVRQRLTM
                     VALPFSQEGAQTGGLAAGPILSARDEYAERSVRNQQEGYGYHSQLATDLARIEPVFTV
                     AENAGIPGALSKFFQSFSVLSVSPNDPANRQVVIDRAQDVATAFNQTAIALVNAGQNA
                     NRQIDSVIDRINSIAGTIQEINLERRQNFRDRGDAGLDARLHVALEELSELVNYSALP
                     QEDGSITVLIGGQTPLVIGDRLYELHADVSGTSEAQILNPDGKNITDQLTSGRLGALL
                     EFRNTVIPSLVDDVNRFAASVAGNVNTVLSQGVDASGQPPALDLFSLDAAAGAALTLR
                     VDPAYSGDDIAASLPPPAPPGGNGNLLELAALPNSKQFAELDNFTFAEFYGKLAGHVG
                     RSLSNARENQNTQSLLLTQARALRSEVSSVSLDEEAVTLIQLQRGYQASAQMVRTLDE
                     LTETVIGMMR"
     gene            6620..7522
                     /locus_tag="VFQ79_23815"
     CDS             6620..7522
                     /locus_tag="VFQ79_23815"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEU0142770.1"
                     /translation="MIRSLSPADDNFLRALSLIQARAERAQRQLATGLKITQASDDPD
                     QLSNLMQARFEIEQLGQIGMNLARVKVEVDTGEQALQSAVKLIDRAIVLGLQGAGGTA
                     TDQTRSTLAIEVEGILEQITNLANSKVEGRYIFSGDSDSTAAFSYDPSDPDNPVGSYQ
                     GTEATRRLLHPAGTTFSVSQTAGEIFNPSGNSVLNALNELRIGLRDNDIARIDAGAAE
                     LRGASTHVNRSLAFYGSVQNRVSDGIEYVNKMELRLQAEVAGIQEADITEAILEFQQA
                     AFHQQAALSSKGSLPKTSLFDYLG"
     gene            complement(7544..8392)
                     /locus_tag="VFQ79_23820"
     CDS             complement(7544..8392)
                     /locus_tag="VFQ79_23820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142771.1"
                     /translation="MCPKWRLAFYLPVFLLAIFFHHSCSSGPGAPEKGTPAFYWQAAQ
                     ERFAAGDYRKTNDHLDQLLKTDNEYTSRAQAWRLVLLTGMTASHMELADSFENGARAN
                     KANPVPFRKYTSDFRNEADRLALQKAETFLDFQKKNKDTEIWLDYGFPTGSAAPVMQL
                     TKASTGILMATDEVETAQRRAVERGVLMATCRTVGAPGDSAKALEVFKSGQVKVPRAQ
                     FMLAIADSLYREAELYTPRKRDQPDRVNTFCNLAMDALKEVPESKESKELKSKIGDTL
                     KKSRKT"
     gene            8781..9266
                     /locus_tag="VFQ79_23825"
     CDS             8781..9266
                     /locus_tag="VFQ79_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0142772.1"
                     /translation="MSTMPTRGFEPEAPRTGSAVIGKSVVIKGQIYSREDLVVDGELE
                     GTIEAHEHRITVGPNGKIQANLKGREVVLLGQVHGNVEATDKIEIRKDARLVGDIKTA
                     RIVIEDGAYFKGSIDISKAEPKAAPQPQRPAGPVTESAPVAAPPPPAANPASMSAERK
                     R"
     gene            9338..9973
                     /locus_tag="VFQ79_23830"
     CDS             9338..9973
                     /locus_tag="VFQ79_23830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0142773.1"
                     /translation="MDSTAHESVRDRYGAAQKPAIPRHSNGLEQFFFAIRDTAGLNIL
                     DLSGASQENINFITNLGHRIYTENFLHIVDSTYQAEDSLSERALQDLGDRVLQQCLDF
                     PDHYFDGALVWDSLQHLKPPVLQIVVGGLFRVLRPNAYLFALFSAQERVQEMDACSYR
                     IADSQTLLLASTGSRPVQFFSTRMLEKIFQQYQTVKFFLTRDHLREVLVKR"
     gene            complement(9974..10930)
                     /locus_tag="VFQ79_23835"
     CDS             complement(9974..10930)
                     /locus_tag="VFQ79_23835"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0142774.1"
                     /translation="MRVLVTGAQGCIGAWSVRKLLDRGVEVVTYDVNAERPRLSLIAS
                     EEELRRVEARVGSIEDTARVKALVRDGGITHIVHLAAVLMPFCQQHPVQGAMVNVIGT
                     LNVFEAARDAGRPVRVVYASSSAVWGPGDAYESRKLNEDDPLKPATHYGVFKQSNEGN
                     ARVFFDSNGISSVGLRPWSVYGVGRDSGLTADPTLAMRALACGEPFEIRLSGHMDLQF
                     VEDVAETFLACLFSPLEGAHVFNLAGDIVEMNELIGLLESMRPGAARLITAKGPQVPV
                     AYRMDDSQLRAKIANIPRTPLKQGVEKTLAHFERLKAEGRLA"
     gene            11035..12159
                     /locus_tag="VFQ79_23840"
     CDS             11035..12159
                     /locus_tag="VFQ79_23840"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0142775.1"
                     /translation="MISRRSFLAAGAAGALPLRPQSTPAARIESISVISQEPGYYCGW
                     ATLAARKNGDLLVAYSGGREAHVCPFGRVELIRSFDGGASWSWPEVLMDTAIDDRDAG
                     VLETPAGTLLVTTFTSLAYEGVQARSKGWEPQRVARWEAVQRRTTPEQRKALLGTWML
                     RSTDGGMAWTAPYRVPVNSPHGPIALKDGRLLYPGKKLWDPGKEVGVAESSDDGKTWR
                     WLSAIPTRPGDKAADYHELHGIQADDGTVIVQIRNHNPENARETLQCESRDGGKTWTT
                     PHAIGVWGLPSHLLRLSNGHLLMSYGYRRAPFGNQARVSTDHGRTWSGPITISDDGIG
                     GDLGYPATVELPGGKLITVWYELMKGSSRAVLRQAVWALV"
     gene            12171..13553
                     /locus_tag="VFQ79_23845"
     CDS             12171..13553
                     /locus_tag="VFQ79_23845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142776.1"
                     /translation="MNYTRRAILSLLGAAAARAQSGLPNVGFRANTEGPRSECGVGAL
                     MPWADSLWAVTYNSHMRTTGTGLGLFRIDETLKAEMVHEHNGTHANRFVHRESNSMFI
                     GPYRIDAKGNMTFMPEFAGHRLTATIRHLSDPANRVYALTMEGLFFEFDVKDLKPRLL
                     FDLVKEMKLAKRPHFKGGCSAQGRVTVANNGFYEYGEDQAGLFEWDGKTWKRLSGKPH
                     MDCAARGNMGQVLFCTGWDEASVLLWALVKGNWQRYRLPKASHAFQHAWQTEWMRIRE
                     VETEHYLMDIHGMFYELQPIPFENAIWGVKPVCQHLRIIPDYCAFRGLLALGGNQTTP
                     NGDNNPVVGQPQSGIWFGKTDDLWSWGKPQGWGGPWRKASVEKDVPSDPFLMTGFQSK
                     MIHVATDRPAEFNIEVDFLGDGSWKPYETVRSGSIGYAHHIFPQGFSAHWVRLVPRQA
                     CTASAEFFYT"
     gene            13586..14095
                     /locus_tag="VFQ79_23850"
     CDS             13586..14095
                     /locus_tag="VFQ79_23850"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0142777.1"
                     /translation="MSQALPEPWLRGPIEGVHPAVGAVLQSFQQAREDLEKWTGDLSK
                     EQLWMKPADLTPVGGQIRHIAGSIDRLSTYLEGRQLSDAQLQELKGEMEPGASLSELS
                     AHLESVLRRVEQLCRGLDPASFPEARAVGRKQLPTTVMGLLIHMAEHTQRHVGQAIVT
                     AKLVRGGTA"
     gene            14124..14624
                     /locus_tag="VFQ79_23855"
     CDS             14124..14624
                     /locus_tag="VFQ79_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142778.1"
                     /translation="MLLLMCGCPNQPNTYASHLRREPIEIPERELRPYVQMGAFEAST
                     HILHGISYDIREQSWRWAADDAALRLRMPAASGVRFVMHFTIPEATFQRTGPVTSSVF
                     VEGREIGSMRCDSPGSYYFDKPVPDGMVPAGTRAEVSIRSDKAWVDPVNNARLSLQMA
                     EAGFVK"
     gene            14621..16333
                     /locus_tag="VFQ79_23860"
     CDS             14621..16333
                     /locus_tag="VFQ79_23860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142779.1"
                     /translation="MTKLQGLGLFVLMTLLFLVANRGAYQGYFHGDDLDNVSWTPGLE
                     NSEYASALVSPKLSPWNFRPVGHFYFAALGRTAGLDYRWYVAVLHGLHLLNVWLIWLL
                     LRRLNLGLVAASAGALVFAFHMGVFEALWKPMYVFDVLCGTFCLLCLLLYVRGNLILS
                     VLSFWLAYKSKEVAIMLPVVLAAWEFWLAEKRWKRLLPFFSVSLLFGLQAIFRNSAKN
                     DDYTFHFTLDAWRITVPFYTSKIFLLPFSGLFTLVFAAVFRDRRVWLGVAALWILLLP
                     LLFLPGRIFQAYLYVPLIGLAIAMAAISARQKTAVVAVFFLLFWIPWNYSQMRIERRK
                     MLTQSDENRAYITQMAQLLRKKPAPKVVVYDTAPEGLQLWGVAASVHIPINDLSTKIY
                     RLEDKEAAEALRNPGVMVVSWDHGARKLWAASRTGNERRTAYIEMKPDTPIWLLTEGW
                     FPREHFYRWTRPRARAGLYRPVDAREFQLIANVPDDQIRATGPFEVSITADGNLIGRH
                     TFKESGLQTVNWRVAAAQPGDVEIELAIDPGFDPSGGNPASLGIAVAGIGFVPPGPSG
                     RVPE"
     gene            16330..17280
                     /locus_tag="VFQ79_23865"
     CDS             16330..17280
                     /locus_tag="VFQ79_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEU0142780.1"
                     /translation="MKLFLGVDGGQSSTTALIGDETGRVLGRGQGGPCNHVGAAEGRK
                     KFTGAIEGCVSAACEQAGLDPRQLHFESACLGFSGGPADKEPILREILSTSSLTVTTD
                     ALIALAGATAGQPGVITISGTGSISFGRGRSGKTARAGGWGYIFGDEGGGFDLTRQAL
                     RAALRFEEGWGPPTSLHGALLAATRAEDANDLLHRFYTADYPRPVIAGFSKLVDEAAC
                     SGDQVAREILMGAAQQLASITAAVRGQLFQPGEAARAAYIGGVFKSRMLLERFRSLVE
                     LEDGNLFGPPEYGPAAGALIEAYRTACIEVVLSNLPDREK"
     gene            17311..18642
                     /locus_tag="VFQ79_23870"
     CDS             17311..18642
                     /locus_tag="VFQ79_23870"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HEU0142781.1"
                     /translation="MIRPNAHSIESLETLIQRNRRGEGGGITSVCSANPQVLEAAARE
                     AAGAGTILCVESTSNQVNQFGGYTGLTPAEFAELVGSITERAGLAREQLLLGGDHLGP
                     YPWRKEPAASAMEKACELVRLCVAAGYSKIHADASMNCADDPGGALDEKVIAERSALL
                     AKAAEEAHSRVPGGPAPRYIVGTEVPVPGGEQSAGEPPPVTRPQDVARTIELARSAFA
                     GQGLQDAWSRVIGLVVQPGVEFGDAVVFDYDPGKTRELVAALPPDPQLSYEAHSTDYQ
                     TGGALRRMVQDHFAILKVGPWLTFAFREAVFALSAVEREWLGGRRSTAVSQVREALEQ
                     AMLQNPEYWRPYYTGSDEDLRFARAFSYSDRCRYYWPQPAVQAELEKLWNNLTASTPP
                     LTLLSQYLPAEYEAVRSGAIANEPKALVQERVRRVLRTYAAACEPVGQSSD"
     gene            complement(18634..19713)
                     /locus_tag="VFQ79_23875"
     CDS             complement(18634..19713)
                     /locus_tag="VFQ79_23875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142782.1"
                     /translation="MPMQRRTFAQALAASALPAQDKRVAVALLTHEAAPHLGAYLEGL
                     AKTEEVSSVYLADAGGKVEPSARKALGAKLAGAYRSPKELFAIHKPQMALVTMEAALA
                     PPAITAALGAGCDVLAEKPACTNLADFEVLVEKARQARRSLVLALANRVDPLLLEAKR
                     VVESGAIGKPYGLDLQMVADQTRLTRPAYHKTWVAQKKRAGGGHLIWLGIHWLDLAMY
                     LTNSRIREVAGFTANVGGQPLDTEDAAVVALRFDNGALGTLTSGYYLDRSYHSLVKIW
                     GSEGWLAVQKHTHKPPLEWYSRGEMHKFEGPAVPAGYTEFVRAIARASAGLDPWPLTV
                     DDSLYVLKTVFAAYRAAESGRTQSV"
     gene            19754..20782
                     /locus_tag="VFQ79_23880"
     CDS             19754..20782
                     /locus_tag="VFQ79_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142783.1"
                     /translation="MREKVRWGVLGVAKIAAVKVIPAMQKGEWSEVTAIASRDIAKAR
                     AAAERLGIGKAYGSYEELLADPDVEAVYNPLPNHLHVPWSTLAARAGKHVLCEKPVAM
                     SVAECRGLMQVRDETGVKIGEAFMVRTHPQWLRARELAHSGGIGELRAIVAAFSYFNR
                     DPGNIRNVPEYGGGGLMDIGCYPIHISRFLFGEEPATVSAAMDRDPEMGIDRLASAIL
                     EFPSGHCTFTCSTQLVPYQRVQILGTKGRVEVEIPFNAPPDRSCRLFVDNGADVFGSG
                     VRTEEFAICDQYTIQGDAFSRAIREDGAVPTPLEDSLSNMAVLEAAFESARTGRTVRV
                     PGLPATER"
     gene            complement(20839..22548)
                     /locus_tag="VFQ79_23885"
     CDS             complement(20839..22548)
                     /locus_tag="VFQ79_23885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142784.1"
                     /translation="MRFLLVLLLAASINLSAQFHELAATDDGRHLYFSSTLIFPGMPV
                     GYFPESRIYRVTPEGIEVFAERGALAPTSQASSGDGARMPQVSSDGQIVGFTLQGICE
                     QQNPCVYVQARAEVRGRRNAVLGPGSLQMSRNGRWALLTSAPGPPDATAETTLIDLET
                     GVRTNVPSPPLQTFAIASDGTVVGTRPLAGTPGPTALGLWRDGQFTPVSLSGPVSIWS
                     ISDNAKFLLYTQLLLTPEGPQPRLLARNISTGGDVVLFEPQRSGQLVYPMGLSQDGQW
                     VLFRVAEGRLEGSAFIASTATGEVWPLPLSKGEMVSEGTLSGWGNAAFLVTTTGRIAR
                     IDLAAGKPLGTTTLVPATPYVSGLIRGAPGSLVRLEGTLPQFAAHLEKSVLLNEQPLP
                     IVFADSNTVGVQVPWEQPVGSAWLRLEVPSESPFQQVDRIMVSEVAPQLEPLAAGETS
                     LFSFKMVRGDFGGLLTTTPKPGEVIHIYATGVGPVASPPRTGEPAPADVLFPTLTDTV
                     CRFEPFTTDAETLFSGLAPGFIGVYQITLRMPEEPVSGPLTGGRCRFGTGTTISFHRL
                     GSP"
     gene            complement(22589..25159)
                     /locus_tag="VFQ79_23890"
     CDS             complement(22589..25159)
                     /locus_tag="VFQ79_23890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0142785.1"
                     /translation="MNRFPWRTAFRIGWREARAAKGKFVFVILAVAAGVGAITGVRGF
                     SEAFRGMLLREARTLMAADLSVRMFEMPDTAQSAAMTALERKGIVRTWITETVSMVST
                     PNLPDPLLVSVKAVEPVLYPFYGQIRLSTGGTLREKLTPDTVVVSADLLLRLDASIGD
                     TIRLGESDYRIAAIVTMEPDRMTGSLNVGPRVMLSREGLDRSKIMVPGSRASQRFLFK
                     LPSPGPGVEAVRAELQRVFPSTLIADFRQTHPLITRGLNRATTFLSLVSLISLIVGAL
                     GVATAVHSHLRQRMDSIAVMKCLGAASPQIIRIYLLQTLSLGLIGSLAGVAVGFLVQA
                     AFPALLSRYFPIGPGIRIDFWTLLQGMSAGILTTLLFTWPPLAGIRRIRPGLILRRDM
                     PEARLSWTERRRNLRSAAVGGAVILAGLGVLAATLAGGSLSYSARIGGWFAGGLFVSL
                     VLMAGFGKLLLAGLKAVVEHGPITLPATVRHGLANLYRPGTQTPFILAALGVGVMFTL
                     TVFLVQRSMLLQMIRSAPPEMPNVFLINVTERERAGLLDLLSKQPGVEKPPEVVAAVS
                     AKLESIDDVPVEKASNDARRRRFYRTRLVTHAEEKPSDTEIVSGQWWDKRNPGPPQVS
                     VSEDAAEVLHIQPGARLEFSASGRTFSAQVSSIHRTEAVRPGSSVEFVFTPGALEGLP
                     AIYYGGMRVRAKDVAALQRAAYRRYPTVTIVNAADVLEIVQEVVDQIAIVVRFVSSFA
                     ILAGAIVLASSVAGTRFRRVREIAIFKALGATRRRVSGIFSIEFIVLGAVAGLMGSIL
                     ATAFSALLLQRFFDAGFRFDPVPNLLAIAFTALLATSAGWLAGYRILGQKPLEVLRNE
                     "
     gene            complement(25156..25845)
                     /locus_tag="VFQ79_23895"
     CDS             complement(25156..25845)
                     /locus_tag="VFQ79_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0142786.1"
                     /translation="MSKHSPMIHASGLTKTIDSGAHTVEILRGIDLEIPRGQFVAIMG
                     PSGSGKSTLLGLLAGLDTATSGRVVIDGNDITGLEEDELARIRGRKIGFVFQSYQLIP
                     TLTAEENVLLPLELAGENHTGVKRARELLGQVGLLDRRDHYPVQLSGGEQQRVALARA
                     FVLRPPIIMADEPTGNLDTANGRMVLDLLIGLNRREETTLVLVTHDQEISARADRRVL
                     LRDGVIAADEL"
     gene            25878..26573
                     /locus_tag="VFQ79_23900"
     CDS             25878..26573
                     /locus_tag="VFQ79_23900"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEU0142787.1"
                     /translation="MRVLIFLAAGLLLAGCRSDREAARQPPRDAADSDMTSPADKRPV
                     IVAFGDSLTAGLGVDAGKGYPDFLQAMLDARGYAYRVVNQGISGDTTSGGVERLDAVL
                     AMKPEIVILELGANDGLRGLPLASTRANLDEMIEKLKQGGARVILAGMTLPRNYGPGY
                     IRDFERIFTDLAKKHSAPLIPFFLAGVAESGEHMQADRLHPTAEGNRVVAANVMKTLE
                     PSLKKGEAGQAPH"
     gene            26639..28186
                     /locus_tag="VFQ79_23905"
     CDS             26639..28186
                     /locus_tag="VFQ79_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142788.1"
                     /translation="MSRDLSSPGTEAYLRWESTSGSVSVELSRELIQMLRGEVYRGVQ
                     ALPRRGAEVGGLLLGRFSSSNETSVRVESIEPVSCEHRFGPSFILSDNDRTGLEQMLA
                     AYESDEEVAVVGYYRSCTGRDIELDEADEELARMYFTDPRQVFLLIKPLSLRECIAAY
                     FYKERGRFLTEGKPSFSLNAEYTSTPTVPEEPAVQPSAPIPPAETRHFEPPIVEPPMV
                     VPDTVRHRRRARREEGREEEEKQPGRLRRLLLTFVAFVAMVGLAFFLGWLNPAREPRF
                     SDLGLNVHQTSNGLEVVWDGAKAASQDARRGVLSITDGASENAVELNRAALERGKFVY
                     EPASNDVLVRLSIYGAAGQPAGESFRVVVVPQKTPPPAKAAPAIEPPPQIAPTPPSVN
                     AARHVKAEAPRIPPPPAAKPARQQTPVASAIAVPAKAIHEVRPGISEGIRSRIRGTVV
                     IAVEVRIDATGKVTSAVPQGRGDVVYTYLASRAEEAAKLWRFEPARTKDGTRIASTKT
                     VYFVFKG"
     gene            complement(28192..30099)
                     /locus_tag="VFQ79_23910"
     CDS             complement(28192..30099)
                     /locus_tag="VFQ79_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0142789.1"
                     /translation="MKRFVELLAAVAVAACLQAPLYSQAPSSPERKAAEERRDYFLAH
                     YTKYEHLIPMRDGVRLFTAVYVPKDTSVPYPILLQRTPYTISPYGSDNYRGIGEDLDK
                     FMREGFIFAYQDVRGRTRSEGEFVNMRPYLTVKRGPKDIDETTDAYDTIDWLVKNVPN
                     NNGKAGVWGISYPGFYAAMSAIDAHPALKAVSPQAPIADWFIGDDWRHNGALFLPHAF
                     DFFIFFGQPWIEPGTSVPPRRFDLGTPDGYEYFLRLGPLRNIRTTLFGREEASWKQLM
                     EHDRYDSFWQARNIRPHLKNIKPAMMTVGGWFDAEDVFGPLAVYQAAEKQSPGANNML
                     VIGPWSHGGWMGRGDRLGNARFASETGRFFREQMMFPFFLRHLKDKEDPKLPEAYVFE
                     TGRNQWRKHDSWPPGQAAEQTFYFDAGGKLSLKAPGNTAQNAFDEYVSDPSKPVPFTD
                     EIAPGMASAYMTSDQRFAARRTDVLVYQTGELEEDFTVAGPIECNLLVSTTGTDSDWV
                     VKLVDIYPGTFPDPEPNPTRVKMGGYQQLVRGEPFRGRFRNSFEKPEAFEPGRVTKVA
                     FTMPDVYHTFRRGHRIAVHIQSSWFPLVDRNPQKFVPIHEATEADFMKATQRVYRSGQ
                     SLSSIKVRTLK"
     gene            complement(30116..31033)
                     /locus_tag="VFQ79_23915"
     CDS             complement(30116..31033)
                     /locus_tag="VFQ79_23915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0142790.1"
                     /translation="MRMSRRDVLIPAAAAPLFGASGQSPAQSPGDRIRLAVSTYSYWH
                     FRTEKYPIEQVIDDAARLGFDGVEILHRQMRDETTSYLNDLKKRAFRNGLALVMLSIH
                     QDFVWPDAAERQKHIAHTKRCIDIAAQLGIPAIRLNSGRWKTIKSFDDLMKVKGQESP
                     LDGYTNEDAVRWCIDSIQQCLPHAEKAGVLLALENHWGLTTEPETLLHIYRAINSPWL
                     GINLDTGNYAGDPYAGIAQLAPEASIVQAKTYYGGGEWYTLDLDYKRIAGILRKAGYR
                     GWVSLEMEGKEPASTAVPKSLKVLREAFA"
     gene            31179..33920
                     /locus_tag="VFQ79_23920"
     CDS             31179..33920
                     /locus_tag="VFQ79_23920"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0142791.1"
                     /translation="MSAQQAVTSATLGGRVEDASAGVLPSVLVTVDDTLRGRNFAART
                     DSGGRYQFLRLAPADYIIRVEDPRFEKYERRLPLLAGQAVDLPIVLRVAGVGQSLAVI
                     AEAPVLESVRTQIGETIGAHDIGGLPLNGRNFLDLALLAPGVSRTNTGTPQQFAETSA
                     VPGTGISIAGQRNLNNAFIVDGLSANDDAAGLAGSFYSQEVIQQFQVVTSGGNAEFGR
                     ASSGVVNIITKSGTNQWHGSAYGFLRNQRFDARNPLATRKDPLTQTQYGASAGGPLIR
                     GRTFLFSNFEQAHRNAAGFITIAPSNVQAVNALLERVAYRGPTLRTGEYATGWDSVNY
                     FARVDHQLTSSNQLTVRYSLYDIASPNARSVGGLNAESRGTRLDNRDQTVALSDMAAL
                     SSRDLNELRVQFTRSRLSAPGNDFIGPAVNIAGIANFGASTTSPTARDNDLYEVSDSI
                     SIQRGAHLFKAGADFLYNRLAIDFPGSLVAAVYSFPSLAAFDAGRYSTFQQAFGEPRQ
                     RQRNPNMGWFAQEEWKPAANWAINAGVRYDLQFLPSPIQLDRNNIAPRIGIAWSPPDR
                     KTVVRASYGLYYDRVPLRATSNALQRDGSRYRVALLAFGQDGAPLFPAQLNAFPAGQF
                     INITTIDPKIQNSYSHQAGLQVERELAHATVLSVGYQWVRALHLILSRNANVPTLSAA
                     DAAALGVPNLGRPDSRYGNIGRYEGAGDSYFNGLFLSLRSRPWKGSELRVSYTLSKTI
                     DDVGNFFFSTPQNNFDLRDDRGLSDNDQRHRLSVAAVLDSPVRSGPGPGRYLLSGWRL
                     SPLFLYTSALPFNAQVNFDRNQDTNLNDRPFGMGRNTGRGFDYASLSLRLARSFSITE
                     RVRGEALAEGFNVLNRVNRALPNNIIGSGTGPPLPSFGRATAVLDPRQIQLGMRFDF"
     gene            33929..35002
                     /locus_tag="VFQ79_23925"
     CDS             33929..35002
                     /locus_tag="VFQ79_23925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0142792.1"
                     /translation="MLTVLAFFTLYLNPASAEVPLRQPQLAADGHTAVLAYGAGKSIF
                     VAVSSDQGATFSKPVKVAEAEVLPLGRHRGPRVAVSGSTIVVTAVVGKRRAEGPHAHG
                     LPGDGDLMAWYSSDRGATWSAGRPVNDVPSAAREGLHTLAGDGRGRVFAAWLDLRRQG
                     TRLYGAFSKDGGATWSRNVLVYESPGGTICECCHPSASFSPSGSLWVMWRNSLEGSRD
                     LYATHSNDFTHFSPAQKLGNGTWLLKACPMDGGGIAAGGNQVVTAWRRESDIYRVEPG
                     KPETMLGAGKDPAIAQSGDQTFIVWNEGGGIVLWSNGGSIRKLSQKGAFPSITGLPGA
                     SAVTAWEQDGGIAVEVVHSGGRH"
     gene            complement(35019..36008)
                     /locus_tag="VFQ79_23930"
     CDS             complement(35019..36008)
                     /locus_tag="VFQ79_23930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142793.1"
                     /translation="MPKHFRLLLLCICFLATALAGGAQTAEVDIGGIVLSVSDSWTAQ
                     VFHIVDQLSEWDDACHRQYGRWAAKALTLDGEDRELLRKHGVLRRARGWNKGFEESFY
                     VDDPIEVAADKAVEKGLLSRDEAAAEKLILLHFAPKLSVLRDAPQIARFKDRLTAEAK
                     RLAPTVQKLVRFSEIEDTVKLPLFLIINPEEGNAGGGFNGGRLVLEIQDRPDPVPTLF
                     HECFHALLWRHKETIEAAAQSAGIRFGALNEGIAYAFGPGLTDNILESDTIAQSLARA
                     LRNREKAWPDAYVQAWAVALVIRPVLRAALDRDETLTSFLPKALAEWRTAVGP"
     gene            complement(36011..36985)
                     /gene="yedA"
                     /locus_tag="VFQ79_23935"
     CDS             complement(36011..36985)
                     /gene="yedA"
                     /locus_tag="VFQ79_23935"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HEU0142794.1"
                     /translation="MPEAKHAQWKLLAAFAAVYIVWGSTYLAIRYAIETIPPFLMAGS
                     RFLLSGSVLLAWSIWRGAERPKWIHWRTALIMGAFMLLVGNGAVTWAEQRIVSGVAAL
                     LIASEPLWLVLLAWAWPGGIAPSKVDLAAIGLGFGGVALLFAPAWSVDNAGGFHLFSA
                     ALILVSAISWAAGSLYGTTAPTSRFSGLANAMTMLCGGALLSITGVLTGEVSHLRPDT
                     ISMASLAAFFYLAIFGSLIGFTSYMYLLAHTTPAKASTYAFVNPVVAVLLGWALAGEP
                     LTRSSVLAMCTIVAAVVLITMGQSRKRKQVRVSDPLEGRKPVAQCAGD"
     gene            complement(36978..37454)
                     /locus_tag="VFQ79_23940"
     CDS             complement(36978..37454)
                     /locus_tag="VFQ79_23940"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0142795.1"
                     /translation="MNNVDLTILKILQEDARISNAELARQLGMAPSAVLERVRKLEER
                     RLIQGYQARLNAMALGWGLVAFVFVRAEEPLGSRETEEKLIAIPEIQEVHHIAGEDCF
                     LVKVRVRDTEALGVLLRDRLGAIPTIRSTRTSIVLTTAKETLAVPVPADREVRAHA"
     gene            complement(37517..37885)
                     /locus_tag="VFQ79_23945"
     CDS             complement(37517..37885)
                     /locus_tag="VFQ79_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU0142796.1"
                     /translation="MAGKNPKELSRRLLRLRASEDMTRVIRRIIRQIPRGKVATYAQV
                     AAAAGYPLHHRQVVQILRKSSGSLPWQRVLGAGGQIRLKGEAASDQRIRLEMEGVRFR
                     GSRVDMREHQHIFRPWDDLE"
     gene            complement(37915..38235)
                     /gene="sugE"
                     /locus_tag="VFQ79_23950"
     CDS             complement(37915..38235)
                     /gene="sugE"
                     /locus_tag="VFQ79_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEU0142797.1"
                     /translation="MAWTILVIAGILEVGWAVGLKYTEGFSRLGPTILTVLAMIASMG
                     LLGIAVRTLPIGTAYAVWTGIGTVGAALFGILVLGESANPLRLICLMLIIGGIAGLKV
                     LSNG"
     gene            38335..39750
                     /gene="rimO"
                     /locus_tag="VFQ79_23955"
     CDS             38335..39750
                     /gene="rimO"
                     /locus_tag="VFQ79_23955"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU0142798.1"
                     /translation="MKIGFVSLGCPKNLVDTEVMMGQLAARGHELSSDPAEAEAIVVN
                     TCSFIDPAKQESVETILEMAGHKKTGRLRRLIVAGCLVERYRDDIRKDIPEVDAVIGT
                     NELEGIVAACEAGESGHNAGASYLYHDLTPRILATPRHYAYIKIAEGCDHPCSFCVIP
                     QFRGRFRSRRFESVVAEATRLFARGVREINLIGQDTTCYGEDLGIKDGLAVLLERLAG
                     IETSGRKWIRFLYCYPNRISQKLLDTIAAHSALVKYIDVPLQHASANVLKRMKRGGSA
                     EIFLRLIEKIRRTIPAVAIRTSMIAGFPGETQSDFEELCNFVQAAQFDRLGVFSYSDE
                     ETSASYRLYGKVDSRTIYQRKRRLMSLQKKISRRRNGALLGEAVPVLVEGASSESEFL
                     WQARLATQAPEIDGVCYITDLGDAPPVPGEIRQMRITRAHDYDLEGVLIDSANEALPV
                     PRAELFPILAAPRARIVPQSL"
     gene            39795..39908
                     /gene="yacG"
                     /locus_tag="VFQ79_23960"
     CDS             39795..39908
                     /gene="yacG"
                     /locus_tag="VFQ79_23960"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEU0142799.1"
                     /translation="MPFCSERCKLIDLGNWASEKYVISSPVVEPPPKSGDE"
     gene            39901..40377
                     /locus_tag="VFQ79_23965"
     CDS             39901..40377
                     /locus_tag="VFQ79_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEU0142800.1"
                     /translation="MSKRAGPVAVALATWFGCGFAPVAPGTAGSLGAIAVAISLDRWL
                     HPAAYWWAIAAVLLSIPAIWAADRTAAGVEKKDPGLVVVDEVVGQWITLAALPSLHWA
                     GVTAAFLLFRLLDIVKPWPARRLESLPGGFGIVADDAMAGLYGALVLLAAGCFNLY"
     gene            40389..41411
                     /locus_tag="VFQ79_23970"
     CDS             40389..41411
                     /locus_tag="VFQ79_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU0142801.1"
                     /translation="MRKDSDGKPQISAVHPAAAIPGGELQIRGKGFAKSTRPQVTIGE
                     LEAPILVGSDSYVVAKVPEGTSEGELVVMNGEKSSVAWACDIGIQIADSVHPVANPVV
                     DPAGNIYTTLSGPRGQKTPVAVYRIDTSYNIKPFITDLMNATGLALDNEGTLYISSRH
                     DGIVYQSSPSGSLSVYVEGMGVATGLAFDADGNLYVGDRSGTVFKISRNRQIYVFATV
                     EASISAYHLTFGPDGYLYLAGPTTSSSDAVHRISSAGEVEVFYKGLGRPQGLAFDADG
                     RLYVAASLGGRRGIVRIDAERNAELFLSGPGIVGLAFAPSRALVVATNNALFRVETGI
                     KGRPLP"
     gene            41426..42673
                     /locus_tag="VFQ79_23975"
     CDS             41426..42673
                     /locus_tag="VFQ79_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU0142802.1"
                     /translation="MQAEIIAVGSELLTPQKIDTDSLYLTGELNALGIEVVAKTVVGD
                     DRERLSTMIRDSLRRSPIVFLTGGLGPTEDDVTRDAVAGALGRELQFNPLVCEGIEAR
                     FRLMNRRMAEVNRRQAYIIAGAEILSNENGTAPGQWVDDNGRIVILLPGPPNELKPMF
                     ARCCLPKLQAAIPRAVIRTRFYRVAGMPESELDQLISPVYTRYSNPVTTILAAPGDIQ
                     IHLRARTETEPQAEALLNELGAQIEELLGRRIYSCNGEPLEAVVGVALRRRGETLSVA
                     ESCTGGLLAGRITSVAGASDYFAGGFTTYTEAMKSSALSIDPELLRVHTAVSQPVAEA
                     MALACRERTGSTYALSVTGVAGPGGGTEQNPVGTVYIGLAGDEGCDVRRMRFLGDRTR
                     VRILAVQSALNLLRLHLDGESGR"
     gene            42677..43216
                     /locus_tag="VFQ79_23980"
     CDS             42677..43216
                     /locus_tag="VFQ79_23980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142803.1"
                     /translation="MRAGIASLLLVGCVLAIACAGRREQRAPSLPDIQRQSVTGAVIP
                     AGTIIVLRIVDPIATAKADDSLGYAAVTARDIPEQSGKVLILAGSPVTLGVAQNSSGL
                     KLVLHSVMINGNNYPVAGKGAPLGAVVQALAPGVESAPPDPVADGDIAVEGASMNVPR
                     GSLLTFQLEQPFTIGEGSG"
     gene            complement(43228..43800)
                     /locus_tag="VFQ79_23985"
     CDS             complement(43228..43800)
                     /locus_tag="VFQ79_23985"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0142804.1"
                     /translation="MLFLPATQRYSSMLYLVIRTVPAPEDLMGPVRRVVESVDDTISL
                     RRMRTLEMQVSDVLWPVRTGARVVAWACGVAVVLAVLGLYGVIAYSISRRQREMAIRT
                     ALGARPVDVLRLVLIQGLSMTLWGIVLGLPLGAVANVALARMLYGLRAVEPAVLMAGI
                     AIWFAISCLACIPPAWRAVRNSPAAIREPG"
     gene            complement(43825..44064)
                     /locus_tag="VFQ79_23990"
     CDS             complement(43825..44064)
                     /locus_tag="VFQ79_23990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142805.1"
                     /translation="MDLIDSEFLTTMKVRFVGGRNFTSHDREGSQPVALADERLVRRL
                     WPDAAAPLEALGRTLRVGKEKSPVQIVGIVASDFR"
     gene            complement(44055..45848)
                     /locus_tag="VFQ79_23995"
     CDS             complement(44055..45848)
                     /locus_tag="VFQ79_23995"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0142806.1"
                     /translation="MMLSRFSAWLHKRLLLFYPAEFRAEFGDEMEQDFSARMAHRTRR
                     EQAAAWAGTFLDWLVSMPREQLEVTRRDLRASARALKRSPLFTLALVLSLGGGIAGAS
                     LVFTLANSLLVQTPLKDPSTFVRVLRGDGTSEPSSWPDYIDYRDRNRTFTHFTAWNIM
                     PVFLGRGDQSQSVMAETVAENYFEMLQIRPFLGRLFHSGDCPSTCAQQVILSHRFWRR
                     VYGGDPSVIGHQVALSGVPATVTGIAPEGFDGTLAPVMTDIWIHVENRRLTNPELFSD
                     RNARWLAIAGRLKPGVSEAQAIADLNAIDRELQQEHRYTERQERRLWPSVTRGIGIPL
                     IRRRVELVVGLLGAVAALVLLISCANVANMLVARATARKQETAMRRALGAGDYRLIRL
                     SLTESLMLAVLGGTVGLLLSIWITGLLPELQPPANDLYTYRVAIHQDFRVWIFTAAIS
                     LFCGLLFGILPALQAARADCLPAIRNQDAQGRPRWFARKVLVSCQVASSVVLLVTAGL
                     FYRSFASQQETGPGFAITRGLIVPLNLNLASYAGNQEKSRRFFDSVKERVSSLAGVES
                     AGLASYVPLNAQASQIEVWRDAGAPVRAGWT"
     gene            complement(45845..46213)
                     /locus_tag="VFQ79_24000"
     CDS             complement(45845..46213)
                     /locus_tag="VFQ79_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0142807.1"
                     /translation="MAFHREDPTDYLPLTPVVLHILLALADEERHGYGIMLEIANRTG
                     GSFRPGSGTLYGTLKRLLSSGLAEESGERPAPDLDDSRRRYYRLTKLGRRVLQAELKR
                     LEQVVGVGRMKKLLPRSAST"
BASE COUNT         9324 a        13904 c        14092 g         9145 t
ORIGIN      
        1 gaagtatccg ggttccaaaa gatgctgttt catcacctca tcgttcaagg taatgcccag
       61 ccccggtcct tcgggtactt ttgcaaatcc gcggttcaca atcggttttt caatgccttg
      121 caccaggtct tcccaccagg gaacatctac cgagtgatgc tccagagcga caaagttctc
      181 ggttgccgcg gcgcagtgca ggttcgccat gaaagaaacg ggagtccccg caaaatgcat
      241 tgccatcgcg acgccgtatt cctgtgcgcc gtctccaatc tttttggttt cgagaatgcc
      301 gccggatgtc gccaggtccg gatggatcat gtccacggcg tgcttcctgc ataattcgaa
      361 gaacggctcc ttcaggtaga tgtcctctcc tgtcagaatc gggatgtcca ccgcatccgt
      421 gatctgcttc atcagttccg tgtgttgcca cgggatcatg tcctcaagcc aggccaggtt
      481 gtacttttcg agcgccttgc ccaagcggat acacgaattc accccgatat gcccgaaatg
      541 gtcggcggac aaaggaactt ccatcccaat aacatcgcgt attccgccgg cataatccgc
      601 catcattccg attcctttgt cggtcagctc gattccggtg aacatgtgct gggtattatt
      661 catttcgcgc agggtcgatc cgacggggcg tgtcagcgcg ccgggaacct tctccaccag
      721 gttggcgccc agatccatct tcaggaaagt gaaaccctgc tccatccgct ttttcatgcg
      781 ctccccgtac actttgggat catgggacga agtggtgtcg gcgtagcacc ggatgcggtc
      841 gcggaacttg ccgccgagca tctgatatac ggggacgtta taggccttac cggccaggtc
      901 ccacagagcc atctccacgc cgcagactcc gcctccctgg cgggcatgtc cgccgaattg
      961 cttgatcttg cggaagacct tatccacgtt gcaggggttt tctccaagca gccggctctt
     1021 caacatcagc gcgtacgttt tgcttgcccc gtcccgcacc tcgcccaatc ccgatatgcc
     1081 ctgattcgta tcgatgcgaa tgacgggaca ggtcatcgga gcgcgcgcca ccacggcaac
     1141 gcgcaggtcg gtgatcttca actcggacgg tttggaacgc gtgttcacgt gctgtgtggc
     1201 ggcgtccagg gtatcgtcgg cccaaaaggc ggttccaagt gctccgccgg ccgcggtcag
     1261 aaacgaacgg cgcccggtga gtttttggag tgttgatttc atctcacgct cccgaagcgg
     1321 ctattctata caactttcca ggctcgcgcc cggtacgcgt gatattctga caggtgcatt
     1381 ccgcactact tacaacaagg agaaacagat cgtgagagtt ggagtgttca ctgcgctgct
     1441 gtcgcagttc tcgctcgatc aggtgatcga gaagatcaag aacctcggga tcgacaccgt
     1501 cgaactcggc accggcaact acccgggcga tccccattgc aagctgtcca tgttggacaa
     1561 cgacgtcgaa ctcaaaactt tcaaacagaa gttgagcgac aacggcatct ccatcagcgc
     1621 cctgagctgt catggcgagc cgttgcaccc gaatgccgaa attcgaaata agaaccggga
     1681 aacacagcgg aaaactatcc ttttagcgga aaaactcggt gtgccggttg ttatcgattt
     1741 ttccggctgc ccgggagata gcgacgactc gaaatatccg aattgggtga catcgccctg
     1801 gcctccgcat tatcaggaac tactccaatg gcagtgggaa aaacaggcgg ctccattctg
     1861 gacggaacga acgaaattcg ccgccgatca cggcgtgaaa atcgccattg agatgcaccc
     1921 cggattcctc gtctacagcc ctgagacgat gttgaagcta cgggccatcg ccggtccgaa
     1981 catcggctgc aactacgatc ccagccacat gttctggcag aacatcgacc cgattgccgc
     2041 catccgcgtt ctgggcgatt ctatcttcca cgtccacgcc aaggacacgc aaatctatca
     2101 tcgcaatctg cccgtcagcg gcgtactcga tatgaaactc tacaccgacg aacgcaaccg
     2161 ggcgtggatc tttcgcacct gcggctacgg ccacggagcc gaatggtggc gcgaattcgt
     2221 ctccacgttg cggatgttcg gctacgatta cgcgctttcg atcgagcacg aggatagcct
     2281 gatgtcgccc aatgaggggc tgaccaaagc cgcggctttt ctcaacgaca tcgtgattcg
     2341 cgaagacacc acggcgccct ggtgggtgtc gaacgtgagc gcctgacgtc acaccgggag
     2401 gtcgccacgc tgtatacggc tggcctcctt ccgagcatcc cccgtttatg gtagcagcgc
     2461 ttaccctcaa aagtggggac gcttcaattt tcagtggttt tgtttgataa tttaagtcaa
     2521 gttccttgct tgtacccgca tcttatgggt taatatggag cttacttgtt ctgcgccgcg
     2581 tatcggttat ggatacgcgc agttcgcctt attggagttc gaatggcatc tgatcagcgc
     2641 accgccaata ataaacttgc accccaactg aatctcgctt tacttcgcga gaagaagaaa
     2701 atctctttac gcgacattga ggatgagacg aaaatcagta tacgtttcct caaagcgatt
     2761 gaagacgaag aattcaacca gttgccgggt gggatcttct caacaagcta cattcgccag
     2821 tacgccgcca tcgtggggca tgacgagacc gagctgatcg agcactatcg gacaaagatg
     2881 ggtcttaatg agccggagga gtctcaaggc ccggggcgca agggaaaacg gcaacaggag
     2941 aaagtctctc gggcttttgc gtttcctccg ttttcctgat cgcgcggcgc ttttcgttct
     3001 ggcgtgtctt gtgggggcgt gttgtgcctc tggggcgagc atcgaaggca ccgttgttga
     3061 aaacagcgcc ggcaagccga tcgcccgcac acgtgtcacg ctggagggca ctgggtcgac
     3121 gagcggaggc gcaaccgtat tatcgagttc ttccggccag ttcgcattcc gttccctttc
     3181 cgctggtacc tacctgctgc gcgccgaaag ggccggattt gccgtcaccc gctacggaca
     3241 gcggcgctgg aatgcgccgg gaacccccat tgtgctggat gccgaaacac agcacatcgt
     3301 tcagatacgt ctgcgcaaac tcggcgccgt aaccggcgag atcggcgacg agaaccagtt
     3361 agggcttccc ggtgtgacag tccatgccta ccgggctgcc tccaagctca ccctcgctgg
     3421 tagcggcgtc accgatgacc gcggcattta tcgcatttcg aacctggagc cgggaaagta
     3481 cctgatccgc tcggccgccc gcctgctcga agatcagcag ggcttgcttc ccaccttttt
     3541 cgggcagacg gcgagtgccg gcgaagcgcg tcccgtgatc gtacagctcg agcaggacac
     3601 taccggagtg aacatccagc ccctgccggg gcggcttgca tcgcttcagg gaaccgtggc
     3661 cagcccggcg gccgttttca tctctttgta cggcgatacc ggggtcttgc ggaagcaggt
     3721 gcctcccggc ggagagttcc atttcgatca gctcgctccc ggaccttaca gtctgctcgc
     3781 ccaggcggga gaccgctccg caccgctgtc gtccttccaa aagatttgga tcggtgggga
     3841 atcccagcgg gtaaccgtcg agttggcgcc gtctccccga ttaatcctgc gctgcgagaa
     3901 caggaacggc aacccgctcc agcacaaggc cgtgtcgatc tttctgcggc gccgggacct
     3961 gaaggactcc accacggacc gggccgtatg cggcgacgta ctgcctctta ctcccggcac
     4021 ttgggaggtg gcggcatcgg cgccgccgga catttatgtc tccgggctgc gaggagcccg
     4081 catctccccg ttcgcgcacg aattcgacat ggtgtccggc cggaatacgg aggtggtgat
     4141 cctcttttct tcggacccgg gctctctgcg aggtacggtg tcgacccggg acggcgctcc
     4201 cgcgattggc gcacctgtct atttcaaccc cgtggacagc gatctgcgca gccggctcgg
     4261 cggcctgcgc accacccgaa ccggcgcaag cgggaagttt tggctcaacg gctttccacc
     4321 cggccggtat gaggtcctca gcacgttctc gccggtcgac ggcgaatcgg aagactggaa
     4381 accgggacgc ggcaagcttg tcacactggg agagggcggc gatatgaccg tcgagatcgt
     4441 tctcgaagac ctggagtgaa aataattgag cgcgggagct aatggatacc tgccctcatg
     4501 ccgatgagat agcgagagag gaacaaaccc gctgaaggcg ggcctcgaac aacgcatgaa
     4561 aatacatgat ccaaacggat acggaaccgg cattcatggc ccgggtgggc cgtcaggggc
     4621 cgatgctgtt ggcaggggca ccagcgcacg aggctccaga caaaccggct tacggaatga
     4681 ccaggtagag atctccaatc tggctggaca tctcagcgca ctgacctccg actcgccgga
     4741 acggctcagc cggatagaga aactgtccgc cgatttccag gcgggccggt atcaggtgga
     4801 tgcagcggtt atcagccgcg ccatggttga ggacggcttg ggagcgcgtt cctgatgtcc
     4861 gggactgccg cgctggagcg tgttcgacgg gccagagatc tcgttgccac gctacagtct
     4921 gagctttcga gtggcggcgt tcaagctttg atgatgagcc cggaccggct ggagcgggcg
     4981 ctggcttctc tggagccggt gagctcggcc ccggcggtgg agactggcga acaggacgtg
     5041 cttctggccg atctcaatgc tttccggcag gacctggcgc gggtccgttc tctgttggta
     5101 caggccgccg gtttctatat aggctgcgag ccgtccgcgg agcaggaggc gggctacggg
     5161 ccgcaggcag gactcctctc tccggatgcg tcgcgggtga tggtaacggg ctgacaatgg
     5221 gcaatttctt ttcttctctg tcttcggtcg cggcaggcat gcgcgtgtat gaacgtgcgc
     5281 tcaacacgat tcagaacaac gtttcgaacg cttccacccc gggatacgtc cggcagagat
     5341 tgacgatggt cgcccttccg ttctcgcaag agggcgcaca gacgggtggt cttgccgccg
     5401 gtccgatcct gagtgcccgt gacgagtatg ccgagcggag cgtaagaaat cagcaggaag
     5461 gctacgggta tcattcgcag ctagcgaccg acctggcgcg catcgagccc gttttcacgg
     5521 tggcggaaaa cgccggtatc cccggcgcgc tcagcaaatt tttccagagc ttttcggttt
     5581 tgagtgtctc gcccaacgat cctgctaacc ggcaggtggt gatcgatcgt gcgcaggacg
     5641 tggcaaccgc cttcaatcag acggcaatcg cactcgtgaa tgccggtcaa aacgcaaacc
     5701 ggcagatcga ttccgtcatc gacaggatca actcgatcgc cggcacgatc caggagatca
     5761 acctggagcg ccgccagaac tttcgggacc gcggcgatgc cggcctggat gcgcgcctgc
     5821 acgttgcgct ggaagagttg tccgagctgg tgaactattc ggccctgccg caagaggacg
     5881 gctccatcac ggtgctgatc ggagggcaga ccccgctggt catcggtgac cggctgtatg
     5941 aacttcatgc cgatgtgtca ggcaccagtg aggcacaaat tctcaatccg gacggcaaga
     6001 acatcaccga ccaactcacc agcgggcggc ttggcgctct gctcgaattc cggaataccg
     6061 tgatcccatc gctggtggac gatgtgaacc gttttgccgc gtcggtagcc ggcaatgtca
     6121 acaccgttct ttcacagggt gtggatgcct ccgggcagcc tccggcattg gaccttttct
     6181 cgctggatgc cgcggccggg gcggccttga cgctgcgtgt ggatcccgca tatagcggcg
     6241 atgacattgc cgcgtcgctc ccgccgcccg cccctccagg agggaatggc aacctgctgg
     6301 agctggcggc cttgcctaat tcgaagcaat tcgccgaact cgacaatttt actttcgccg
     6361 aattctacgg caagctggcg gggcatgtgg gccgcagttt gtcgaacgcg cgggaaaacc
     6421 agaacacaca gtcgttgctt ctcacgcagg cacgggcgtt gagaagcgaa gtctcgtcgg
     6481 tttctctcga cgaagaagcg gtcacgctga tccagcttca gcgcggctac caggcctctg
     6541 cgcaaatggt aaggacgctg gacgagctga ccgaaaccgt tatcggaatg atgcgatgag
     6601 cttcggtagg agactataga tgatccgttc cctcagtccc gcggatgata atttcctgcg
     6661 ggctctttcc ctcattcagg cgcgcgcaga gcgtgcacaa cggcagctcg caaccggcct
     6721 caagatcacg caagcttccg atgaccctga ccagctatcc aacctgatgc aagcccgctt
     6781 cgaaatcgag cagcttggtc aaatcgggat gaatctggcc cgggtgaaag ttgaagtgga
     6841 caccggggaa caggcgctac agtccgctgt caaactcatt gatcgggcga tcgtgctggg
     6901 attgcagggc gcggggggaa ccgccacgga tcagactcgc agcaccctcg ctattgaagt
     6961 cgaaggaatt ctggagcaaa tcacgaacct ggcgaactcc aaagtggagg gccgctacat
     7021 tttcagtggc gatagcgata gcactgccgc attttcgtac gatccctccg accccgataa
     7081 cccggtcggc agctaccagg gaacggaagc cacccgccgg ttgctgcatc cggctggaac
     7141 aacgttttcg gtttcccaaa ctgccgggga gatatttaat ccgtccggta acagcgttct
     7201 aaatgcgctc aacgagttga ggatcggcct ccgcgacaac gatatcgccc gcattgacgc
     7261 gggagccgcc gaattgcgtg gcgcctcgac tcacgtaaac cgcagcctgg ccttctacgg
     7321 ttcggtgcaa aaccgggttt cggatggaat cgagtacgtc aacaaaatgg aactgcggct
     7381 acaagccgag gtcgcaggga tacaggaagc cgatatcacc gaggcgatcc tggaattcca
     7441 gcaggcggca ttccatcaac aggcggcgct cagctcaaag ggcagtcttc cgaagacaag
     7501 cctgttcgat tatctcggtt aaatcgcagc gcaagccgga gactcaggtc ttccgcgatt
     7561 tcttgagagt atcgccgatc ttagacttca gctctttgct ctccttcgac tccgggactt
     7621 ccttcaaagc gtccattgcc aggttgcaaa acgtattgac gcggtcgggc tgatcccgct
     7681 tccgcggggt gtacaattcg gcctcccggt atagtgaatc cgcgattgcc agcataaact
     7741 gcgcccgcgg cactttcacc tgaccgcttt tgaacacttc cagcgccttg gctgagtccc
     7801 cgggagcgcc gacagttcgg caggtggcca tcaggacgcc gcgttccacc gcgcggcgct
     7861 gtgcagtctc cacctcatcg gtcgccatca gaattccggt gctcgctttg gtcaattgca
     7921 tgacaggcgc cgcgctgcca gtcgggaaac cataatccag ccaaatctcg gtatccttgt
     7981 tcttcttttg gaagtccaga aaggtctccg ccttttgaag agccaggcgg tccgcttcgt
     8041 tgcggaagtc ggaagtgtac ttccggaagg gaaccgggtt agctttgttg gcccgtgcgc
     8101 cgttctcaaa gctgtccgcc agttccatgt ggcttgctgt cattccggtc agaagcacaa
     8161 gccgccacgc ctgggcgcgg ctcgtatact cgttatccgt tttgagcaac tggtcgagat
     8221 ggtcgttcgt cttccggtaa tcaccggccg cgaaccgctc ctgagctgcc tgccagtaaa
     8281 aggcgggtgt gcccttctcc ggggcaccgg gcccggacga gcaggaatgg tgaaaaaaaa
     8341 tcgccagaag gaacactggc aggtaaaaag cgagtcgcca tttgggacac atttgtctgg
     8401 tctcctaagc ggaattggat atcacaaagc tgccttcggc gcaagtgcca ctgtctccgc
     8461 tcgtccgccg tcccttcctg cttcaggaac cgggggatgg gcttctccgg gtttcagaac
     8521 aaaatcatgg tgcggatggc ttgttttatg ttagacctga tagtactgga gcattgcctt
     8581 ttcctttagg cttatctagt atgaaagatc gaagcaaaca ctggtgaagt tgccaactta
     8641 ctaccgggga aggagttctt ccagagatgt ggaatagaag gaaagatgag gacgttcgaa
     8701 aaccgccgcc ggcggcacct tttcgtccgg tttcgaaccc gcagtcaagt tccaccgagt
     8761 ttccaaagga ggggatgcct atgtcaacca tgcctacacg aggtttcgag cctgaggcac
     8821 cgcgaaccgg atctgccgta atcggcaagt ccgtcgtcat aaaaggtcaa atttatagtc
     8881 gcgaagatct ggtcgtcgac ggtgagcttg aaggcacaat cgaggcgcac gagcaccgca
     8941 tcaccgtcgg gccgaacggc aagattcaag ccaacctgaa aggccgcgaa gtagtcctgc
     9001 tcggccaggt acatggcaat gtcgaggcca ccgacaagat cgagatccgc aaggatgccc
     9061 ggctggtcgg cgatatcaaa acggcgcgta ttgtcattga agatggcgcc tacttcaagg
     9121 gaagtatcga tatttcgaag gccgaaccga aagctgcacc gcaaccacag cgtccggcgg
     9181 gtccggtgac tgaatcagcg ccagtagcgg cgccgcctcc tccagcggcg aatccggcgt
     9241 ccatgtccgc cgaacggaaa cggtaagcta aggaggctgg gacatacaat tgttgcggga
     9301 gcggttgaag agcctcatcc atgccgggcg gaatggaatg gattccaccg ctcacgaatc
     9361 cgtgcgcgac cgctacggag cagcgcagaa accggccatt ccgcgccata gtaatggcct
     9421 cgagcagttt tttttcgcga tccgggatac ggccggcctg aatatcctgg atctgtcggg
     9481 cgccagccag gaaaatatca atttcatcac gaatttgggc caccgtatct atacggagaa
     9541 cttccttcac atcgtcgact ccacctacca ggctgaggat tctctttccg aacgtgcgct
     9601 tcaggacctg ggagaccgcg tattacagca atgtcttgat tttcctgatc actacttcga
     9661 cggagcgctg gtgtgggata gcctccagca tttgaagcct cccgtgcttc agattgtcgt
     9721 gggtggcctt ttccgggtgc tgcggcccaa tgcttacctt ttcgcgcttt tcagcgcaca
     9781 ggaacgggtg caggagatgg acgcctgctc ctatcgcatc gcggactccc aaacgctgct
     9841 gctagcctcc acgggttcga gaccagtgca gtttttcagc acgcggatgc ttgaaaagat
     9901 cttccaacag tatcagacgg tgaagttctt tctcacgcga gaccacttga gggaagtact
     9961 cgtaaaacga taatcaggcc agacgcccct ctgccttcag gcgctcgaag tgtgcgagcg
    10021 ttttttccac gccctgcttc agcggagtgc ggggaatatt tgcgatcttc gcgcgcaatt
    10081 gcgaatcgtc catcctgtag gccaccggca cttgcgggcc ttttgcggtg atcagccgtg
    10141 ccgcccccgg acgcatcgat tcgagcagtc caatcaactc gttcatttcg acgatatcgc
    10201 cggcgagatt gaacacgtgt gcgccttcga gcggggagaa caggcacgcg agaaatgttt
    10261 cggcaacgtc ttcgacgaat tgcaggtcca tgtgtcccga caagcggatt tcaaatggct
    10321 cgccgcaagc caaagcgcgc atggcgagcg ttggatccgc ggtgagtccg ctatcgcgcc
    10381 ccacgccata gacgctccag ggcctcaggc ccacactcga gatgccgttc gagtcgaaga
    10441 atacccgtgc attcccttcg ttggactgtt tgaaaacgcc atagtgtgtc gcgggtttga
    10501 gcggatcgtc ttcattcagc ttacggctct cgtaggcgtc tccggggccc catacggccg
    10561 aggaactcgc atagacgacg cggacaggcc tgccggcgtc gcgtgccgct tcaaatacgt
    10621 tgagagtgcc gatgacgttc accatcgcac cctgtaccgg gtgctgctgg cagaagggca
    10681 tcagcacggc ggcgagatga acgatgtgag taatgccacc gtcacgaacc agagctttca
    10741 cgcgggccgt atcttcgatc gaacccaccc gggcctccac ccggcggagt tcctcttctg
    10801 acgcgatgag cgaaagcctg ggccgctcgg cattcacatc gtaagtcacc acctcaacgc
    10861 cgcgatccag cagctttctt acggaccagg cgccaatgca tccctgcgcc cctgttacca
    10921 gtacacgcat cccatctcca gaaataaatc gatcggtacg acctccgaat gttagcacgg
    10981 gcccggcgca tgccggagca ggccgtaaat tgatattgtg atcgggggac aagcatgatc
    11041 tctcgcagat cttttctggc cgccggggcc gccggggcct tgccgctgcg gccgcagagc
    11101 acgccggcgg cgcgcatcga atccatttcg gtcatcagcc aggagcccgg ctattattgc
    11161 ggatgggcca cgctcgccgc gcgtaaaaac ggcgatctgc tggtggcgta ctccggcgga
    11221 cgggaagcgc atgtctgccc gttcggccgc gttgagctga tccgctcttt tgatggaggc
    11281 gccagttgga gctggcctga agtgttgatg gacaccgcca tcgacgatcg tgacgccggc
    11341 gtactcgaaa cacccgcggg tacactgctg gtcacaacgt tcacatcgct cgcttacgaa
    11401 ggtgttcaag cccgctccaa agggtgggag ccgcagcgcg tagcccgatg ggaagccgtc
    11461 cagcggcgga ccactccgga acagcgcaaa gccctgcttg gaacctggat gctgcgatcc
    11521 accgacggcg gcatggcctg gaccgcgccg taccgcgttc cggtgaacag cccgcacggg
    11581 cccatcgcgc tgaaagacgg ccgcctgctc tatcccggta agaagctttg ggaccctgga
    11641 aaagaagttg gcgtggcgga gtcttcggac gacggcaaaa cctggcgctg gctttcggcc
    11701 attccaacgc ggcccggcga caaggcagcc gattaccatg agttgcacgg cattcaggcg
    11761 gacgatggaa ccgtcatcgt tcagattcgc aatcacaatc ccgagaacgc gcgcgagacg
    11821 ctccagtgcg aatcgagaga cggcggcaag acctggacca cgccccacgc catcggtgtg
    11881 tggggcctgc cttcgcattt gctgcgttta agcaacggac atctgctgat gagctacggt
    11941 tatcgccgcg cccccttcgg caaccaggcg cgtgtaagta cggatcatgg acgcacatgg
    12001 tccggcccaa ttacgatctc tgacgatggc atcggcggcg acctcggata tccggctacg
    12061 gtcgagttgc ccggagggaa gcttatcacc gtgtggtatg agctgatgaa gggatcttcg
    12121 cgcgctgtgc tgcggcaggc ggtgtgggcg ctggtgtagc tgtccggatt atgaattata
    12181 caagacgtgc aattctttca ctactcggtg cggcggctgc tcgggcgcaa tccggtcttc
    12241 ccaacgttgg ctttcgcgcc aacacggaag gccctcgctc ggaatgcggt gtcggcgctc
    12301 tcatgccgtg ggcggactcc ctttgggccg tcacctacaa ctcccatatg cgtacaaccg
    12361 gaaccggact cggcctcttc cgtatcgacg aaaccctgaa agccgaaatg gtgcatgagc
    12421 ataacggcac gcatgccaac cggttcgttc atcgcgaatc gaacagtatg ttcatcggcc
    12481 cataccggat cgacgccaaa ggcaatatga ccttcatgcc ggagtttgcc gggcatcgtc
    12541 tgacggccac cattcgccat ttaagcgacc cggcgaaccg cgtatacgca ctgacgatgg
    12601 aaggattgtt cttcgaattc gacgtgaagg acctgaagcc gcggctgttg ttcgacctgg
    12661 tgaaggaaat gaaactcgcc aaacgtccgc acttcaaggg cggctgttcg gcacagggcc
    12721 gtgtgacggt tgcaaacaac ggattctacg agtacggcga agatcaggcc ggcctgtttg
    12781 agtgggatgg caaaacgtgg aagcggctca gcggcaagcc ccacatggat tgcgcggcgc
    12841 gcggaaacat gggacaggtc ctgttctgca ccggatggga tgaggcatcg gtgctgttgt
    12901 gggcgttagt aaaaggaaac tggcagcgct atcgtctccc caaagccagc cacgcgttcc
    12961 agcatgcctg gcaaaccgaa tggatgcgta ttcgggaagt ggagaccgag cattacctga
    13021 tggacatcca cggcatgttc tatgaattgc aacccattcc tttcgagaac gccatctggg
    13081 gcgtgaagcc ggtatgccag catctgcgca tcatcccgga ctattgcgcg ttccgcgggc
    13141 tgctcgcgct cgggggcaac cagacaactc cgaatggaga caacaatcct gtcgtcggcc
    13201 agccgcaatc gggcatctgg ttcggcaaga ccgacgatct ctggtcatgg ggcaagcccc
    13261 aaggatgggg cggcccctgg cgcaaggcat cggtggagaa agacgtcccc tccgatcctt
    13321 ttctgatgac cggtttccaa tcgaagatga tccacgtcgc cacggatcgc cccgccgagt
    13381 tcaacatcga ggtcgatttc ctgggcgatg ggagctggaa gccttatgag acagtcaggt
    13441 ccggcagcat cggctacgcc caccacattt tcccgcaggg tttctccgca cactgggttc
    13501 gtcttgtgcc gcggcaagcc tgtacggcgt cggcggagtt tttctatacg tagctcgatc
    13561 gcattaagct gctaccatcg tttcaatgag ccaagcatta cccgaaccat ggcttcgcgg
    13621 gccgatagaa ggggtgcatc ccgccgttgg cgctgttctt caatcgtttc agcaggcgcg
    13681 ggaagatctc gaaaaatgga ccggggacct ctccaaggaa cagttatgga tgaagccggc
    13741 ggatctcacg cccgtgggcg gccagatacg ccatatcgcc ggaagtatcg acaggttgtc
    13801 tacgtatctg gaaggccgtc agctcagtga cgcacagctt caggagctga aaggggagat
    13861 ggagccaggc gcgagtttga gcgaactttc cgcgcattta gagtccgtcc tgcgccgcgt
    13921 ggagcagctt tgccgcggac tggatcccgc gagttttcca gaggcgcgcg ccgttggacg
    13981 caagcaattg ccgaccacgg tcatgggact actcattcat atggcggagc acacgcagcg
    14041 gcatgttgga caggcgattg taaccgccaa gctggtgcgt ggtgggaccg cttagccgga
    14101 ctgccggcac tttctggata cctgtgttac tgctgatgtg cggttgtccg aatcagccga
    14161 atacctatgc gtcgcacttg cgccgcgaac cgatcgagat cccggagcgc gaactcagac
    14221 cgtacgtcca gatgggagca tttgaggcgt caacgcatat cctccacgga atttcctacg
    14281 acatccgcga gcagtcgtgg cggtgggctg ctgatgacgc cgcgctgcgc ttgcgaatgc
    14341 cggcggcatc cggcgtgcgg ttcgtgatgc atttcacaat tcccgaagca acgtttcagc
    14401 gcacaggccc cgtgacatcg tccgtcttcg tcgagggccg cgagatcgga tcgatgcgtt
    14461 gcgattcgcc cggcagctac tatttcgata agccggtgcc ggacggaatg gttcccgccg
    14521 gtacacgcgc cgaggttagc atccgctcgg acaaagcctg ggtggacccg gtcaacaacg
    14581 cgcggctgag tcttcaaatg gccgaagcgg gattcgtgaa atgacaaaac tccagggact
    14641 agggttgttc gtgttgatga cgctgctgtt cctggtggca aaccgcggcg cctaccaagg
    14701 ctactttcat ggcgacgatc tcgacaatgt cagttggact cccggcctcg agaactcgga
    14761 atacgcctcc gccctggtgt cacccaagct ctcgccgtgg aacttccggc ccgtcgggca
    14821 tttctatttc gcggcgctgg gtcgcacggc gggcctggac tatcgatggt acgttgccgt
    14881 gctgcacggt ttgcatctgt tgaatgtctg gctgatatgg ctgttgctgc ggcggctgaa
    14941 ccttgggctg gtcgccgctt cggcgggggc gctcgtcttc gcattccaca tgggcgtatt
    15001 cgaggcgtta tggaaaccca tgtatgtttt cgatgtgctc tgcggcacgt tctgtctgct
    15061 gtgcctgttg ctgtatgtgc gcggaaatct gattctgagc gtgcttagtt tctggctggc
    15121 atacaaatcg aaagaggtag cgatcatgct gccggttgtg ctggcggcgt gggaattctg
    15181 gctggcagag aagcggtgga aacgtctcct tccgttcttt tcggtctcgc ttctcttcgg
    15241 gctacaggca atcttccgga acagcgccaa gaatgacgac tacacgttcc acttcacgtt
    15301 ggatgcgtgg cggatcactg ttccttttta tacgtcgaag atattccttc tgccgttttc
    15361 cggactgttc acgctggtgt ttgccgctgt ttttcgcgac cgccgggtat ggctgggcgt
    15421 ggcggcactc tggatacttc tgctgccgct gctgttcctt cccggcagaa tctttcaggc
    15481 ctacttgtac gtgccgctca tcggcctggc catcgcgatg gccgctatca gcgcacggca
    15541 gaaaactgcc gtggttgcgg tgttctttct gttgttctgg attccgtgga actacagcca
    15601 aatgcgaatt gagcgccgca agatgttgac gcagtccgat gagaatcgcg cgtatatcac
    15661 gcaaatggcg cagttgctgc gaaaaaagcc cgctccgaag gtagtggtgt atgacacggc
    15721 gcccgaaggc ctccaactgt ggggcgtggc cgcttcggtt cacatcccga tcaatgacct
    15781 gagcaccaaa atctaccgcc tcgaggataa agaagcggca gaggcgctcc ggaaccccgg
    15841 cgtgatggtt gtaagctggg atcacggcgc aaggaagtta tgggctgcat cgcgcacggg
    15901 caacgagcgc cgcacggctt acattgagat gaagcccgac acgcccatct ggctgctgac
    15961 ggaaggctgg tttccgcgtg agcacttcta ccgctggaca cggccccgcg cgcgagccgg
    16021 cttataccgg ccggttgatg cccgcgagtt tcaactgatc gcgaacgttc cggacgacca
    16081 gatccgcgcc accggaccgt tcgaggtctc gatcacagcg gatggcaatc tgattggccg
    16141 ccatacattc aaagaatccg ggctgcaaac ggtgaactgg cgggttgccg ccgcgcagcc
    16201 tggcgatgtt gaaatagagc tggcgataga tcccggattt gacccttccg gcggcaatcc
    16261 ggcgtcgctc ggtatcgccg tggccggaat tggattcgtg ccgccagggc catccgggag
    16321 ggtgcccgag tgaagttgtt tctcggagtg gacggaggac agagcagcac cacggctctg
    16381 atcggcgatg aaacaggacg cgtgctaggc aggggtcagg gcggaccctg caaccacgtc
    16441 ggcgcggcgg aagggcgcaa aaagtttacc ggagcaattg aaggctgcgt gtcggcggcc
    16501 tgcgagcaag ccgggctcga tccgcggcag cttcatttcg aatccgcgtg cctgggattc
    16561 agcggagggc cggcggacaa ggaaccgatt ctgcgggaga ttctgagcac ctcttcgctt
    16621 acggtgacca ctgatgcact gatcgcgctg gcgggcgcaa ccgccggcca accgggcgtg
    16681 atcacgatct cgggtacagg ctcgatttca ttcgggcgcg gccggtcggg taagaccgca
    16741 agggccggag gctggggcta cattttcggc gacgaaggcg ggggattcga tctcacacgg
    16801 caagccctgc gcgccgcgct gcgcttcgaa gagggttggg ggccgcccac cagcctgcat
    16861 ggcgcactcc tcgcggcaac gcgcgcggag gatgccaacg atctgctgca ccgcttctac
    16921 acggcggatt atccgagacc ggtgatcgcg ggcttctcca agttagtgga tgaagccgcc
    16981 tgttcgggag accaggttgc gcgggagatt ctcatgggtg cggcgcagca gttggcaagt
    17041 atcacggccg ccgttcgcgg ccagttgttt caaccgggcg aagcggcgcg cgccgcttat
    17101 atcgggggcg tgttcaagag ccggatgctg ctcgaacgct ttcgcagtct cgtcgagctc
    17161 gaagacggca atcttttcgg cccgccggaa tatggccccg ccgcgggcgc gcttatcgaa
    17221 gcctatcgca ccgcgtgcat cgaagtcgta ttgagcaacc tgccggatcg tgagaaataa
    17281 cagcgcggtt tcctgcatac tagtgggttc atgatccgtc cgaatgcgca ctcaatcgaa
    17341 tcccttgaaa cgttaatcca acgaaaccgc cgcggtgaag gcggcggcat tacttctgtc
    17401 tgctccgcca accctcaggt gttggaagcg gcggcccgcg aagccgccgg ggccggaacc
    17461 attctctgtg tcgaatccac atcgaaccag gtgaaccagt ttggcggcta cacgggcctg
    17521 acgcccgccg aattcgcgga gttggtcggc agcatcacgg agcgtgccgg gctcgcgcgt
    17581 gaacaattgt tgctcggtgg ggatcacctg ggaccctatc cctggcggaa ggaaccggcc
    17641 gcatcagcaa tggagaaggc ttgcgaactg gtccgccttt gcgtggcagc ggggtattca
    17701 aagattcacg ccgatgccag catgaattgc gccgacgacc ccggcggcgc actcgatgaa
    17761 aaggtgatcg cggaacgcag cgcgctgctc gccaaggccg cggaagaggc gcacagccgt
    17821 gttcccggcg gccccgcgcc gcgttacatc gtagggacgg aggtgcccgt gcccggaggc
    17881 gaacagtccg cgggggaacc gccacccgtc acccggcccc aggacgtcgc ccgcaccatc
    17941 gaactcgccc ggagcgcttt cgccggacaa gggcttcaag atgcatggag ccgcgtcatc
    18001 ggcctggtgg tgcagccggg cgtcgaattc ggcgatgcgg tggtgttcga ttacgatccg
    18061 ggcaagaccc gcgaactggt ggccgcgcta ccgccggatc cgcaattgag ctacgaagcc
    18121 cactcgacgg actatcagac cggcggcgcg ctccgccgca tggtacagga tcacttcgcg
    18181 atattgaaag tcggaccgtg gctgacgttc gcattccgcg aagcggtgtt tgccttatcg
    18241 gcagtcgagc gggagtggct cggcgggcgg cgaagcaccg ccgtgtctca agtgcgcgaa
    18301 gccctggagc aggcgatgct gcaaaaccct gaatactggc ggccgtatta caccggtagc
    18361 gatgaggacc tgcgttttgc aagagccttc agttacagtg accgttgccg ctactactgg
    18421 ccgcagcccg ccgtgcaagc ggagctggaa aagctgtgga acaacctgac cgcctcgacg
    18481 ccaccattga ctctgctgag ccagtacctt cccgcggagt atgaagcggt gcgatccggc
    18541 gcaatcgcaa acgaaccgaa agcgctggtt caggaacgcg tccggcgcgt gttgcgaacc
    18601 tacgccgcgg cttgcgaacc ggtggggcaa agctcagact gattgcgttc tgcccgattc
    18661 cgcggcgcgg taggccgcaa acaccgtttt caaaacatag aggctgtcgt caacagtcaa
    18721 aggccagggg tcgagacccg ctgaggcacg ggcgatggcg cgaacgaatt ccgtgtagcc
    18781 ggcaggtacg gccgggcctt cgaacttgtg catttcgccc cggctgtacc attcaagcgg
    18841 cggcttatgg gtatgcttct gtaccgccaa ccagccttcc gagccccaga ttttgacgag
    18901 cgaatgatag ctgcggtcca gataatagcc tgatgtcagg gtgcccaacg cgccgttgtc
    18961 aaaacgcagg gcgaccacag ccgcgtcttc ggtgtcgagc ggctgtccgc ccacgttcgc
    19021 tgtgaagccc gctacctcgc gaatccgtga gttcgtcaga tacatggcaa gatcgagcca
    19081 gtgaatgccg agccagatca ggtgcccgcc accggcacgc tttttctgcg cgacccacgt
    19141 cttgtggtaa gcgggacgtg tgaggcgcgt ctggtccgcc accatttgca gatcgagacc
    19201 atagggcttg ccaatggctc cggattcgac gacgcgcttc gcttcgagca ggaggggatc
    19261 gacgcgattg gcaagcgcga gaaccagcga acgccgcgcc tgccgggctt tttcgaccag
    19321 cacttcaaaa tccgcaaggt tcgtgcaggc gggcttctcg gccagcacgt cgcagcccgc
    19381 gccaagtgcc gcggtaatgg ccggaggtgc cagagcggcc tccatcgtga cgagagccat
    19441 ttggggcttg tggatggcga agagctcctt cggcgaacgg tatgcgcctg ccagctttgc
    19501 gccgagcgcc tttcgcgcgg acggctcgac tttaccgccg gcatccgcga gatagaccga
    19561 agagacctct tcggttttgg ccaacccttc caggtacgct ccaagatgcg gcgcggcttc
    19621 atgcgtcagc agggcgaccg cgacacgctt gtcttgcgct gggagagcgg aggccgcaag
    19681 ggcctgtgcg aaggtgcgtc gttgcatcgg cattgaagtt atcacaggcc ggcagccgtg
    19741 ggaaactcgc tatatgcgcg aaaaggtacg ttggggtgtt ctcggtgtcg caaagattgc
    19801 ggccgtgaag gtgattccgg caatgcaaaa aggggaatgg agcgaggtca ccgcgattgc
    19861 ctcgcgcgat atcgccaaag ccagagccgc cgcggagcgg ctgggcatcg ggaaggcgta
    19921 cgggtcctat gaagagctgc tcgcggaccc ggatgtggag gcggtctata atccgttgcc
    19981 gaatcacctg catgtgccgt ggtccacgct tgcggcccgc gcggggaagc acgtgttatg
    20041 cgaaaagccg gttgcgatga gcgtggcgga atgccgcggg ctgatgcaag tgcgcgacga
    20101 aacgggtgta aagatcggtg aagcgttcat ggtccgcacg catccgcaat ggctgcgtgc
    20161 gagggagttg gcgcattcgg gcgggatcgg cgaactgcgc gccatcgtgg ctgcattcag
    20221 ctacttcaat cgcgatccgg gtaacatccg gaacgtgccc gaatatggcg gaggcggact
    20281 gatggacatc ggctgttatc ccatccacat ttcgcgcttt ctgtttggtg aggaaccggc
    20341 gaccgtcagc gccgcgatgg accgcgaccc ggaaatgggc atcgaccgtc tggcctctgc
    20401 gattctggaa tttccgtccg gccattgcac cttcacctgt agcacgcaac tggtccccta
    20461 tcaacgagtc cagatccttg gcaccaaggg ccgcgtggag gtggagatcc cattcaacgc
    20521 gccgccggac cgctcctgcc gcttgtttgt ggacaacggg gccgacgtgt tcggaagtgg
    20581 agtccggacg gaagaatttg cgatttgcga ccagtacacc attcagggag acgctttttc
    20641 tcgcgctatt cgagaagacg gggcagtgcc cacgcctctc gaggattccc tctcgaacat
    20701 ggccgtcctg gaggcagcgt tcgaatcggc gcggaccggc cggacagtgc gggttcccgg
    20761 tcttccggca acggagcggt agacccggcg gcctcgccaa ccgcttgctt gcgcgcgcgg
    20821 ctcagctaca agtcgtggct atggagaccc gagccggtga aaggaaatag tggttcctgt
    20881 tccgaagcgg caacgcccgc ccgttagtgg tcccgaaacg ggttcctccg gcatgcgaag
    20941 cgtgatctgg tacacgccga taaaaccggg cgctaatcct gaaaacagtg tctctgcatc
    21001 cgtggtgaaa ggctcgaacc ggcagactgt gtctgtcaat gtgggaaaca gaacgtcggc
    21061 aggcgcgggc tcccctgtcc ggggtgggct cgcgacgggc ccgactcccg tggcgtagat
    21121 atggatcacc tctccgggtt tcggggttgt tgtgagcagg ccgccaaaat cgccgcgcac
    21181 catcttgaaa ctgaacaggc tggtttcacc ggcggcgagc ggttcaagct ggggcgcgac
    21241 ctctgaaacc attatccggt ccacctgctg gaagggagat tcggatggta cttccagacg
    21301 gagccaagcg gatccaaccg gctgttccca aggaacctga acgcccacgg tattcgaatc
    21361 agcaaagact attggcaacg gctgttcatt gagtagaacg cttttctcca aatgcgcggc
    21421 aaattgcggc agcgttcctt cgaggcgtac gagcgatccg ggggcacccc ggatcaagcc
    21481 actcacgtag ggagtggcgg ggacgagcgt ggtagtgccg agaggcttgc cggcggcaag
    21541 atcgatgcgt gcgatacggc ccgtcgttgt gaccaggaag gccgcattgc cccatccact
    21601 caaggtgccc tcgctaacca tctctccttt agagagcggc agaggccaca cttcgcccgt
    21661 ggccgtcgaa gcgataaaag cagatccttc cagacggccc tcggccacac gaaacagcac
    21721 ccactgcccg tcctggctga gccccatcgg ataaaccagt tggccggagc gctggggctc
    21781 gaagagcaca acgtctccgc cggttgaaat attgcgcgcc agaagccggg gctgcggccc
    21841 ttccggtgta agcaagagct gcgtgtacaa caggaatttc gcgttgtcgc tgatggacca
    21901 gatggagacg gggcccgaga gcgagacagg agtgaactgt ccgtcgcgcc agaggccgag
    21961 cgctgtggga ccaggtgttc cggccaaggg ccgcgtacct actacggtgc catccgatgc
    22021 aatggcgaac gtttgcaaag gaggcgatgg aacattggtt cgcacccctg tttcaagatc
    22081 gatcagggtt gtctcggccg tcgcatccgg ggggcccggc gctgacgtca gcagagccca
    22141 acggccgttg cggctcatct gcaaggaacc gggcccgagc acggcgttcc gccggccgcg
    22201 cacttccgcc cgcgcctgta cgtatacgca gggattctgc tgttcgcata tgccttgcag
    22261 ggtgaagcca acgatttgcc cgtcgcttga aacctgtggc atgcgcgcgc catcgccgct
    22321 gctggcctga ctggtgggag caagcgctcc acgctcggcg aagacctcga tgccctccgg
    22381 tgtcacacgg tagatgcgcg actcaggaaa gtacccgacc ggcatgcccg gaaagatcaa
    22441 agtcgaggag aagtaaagat ggcggccgtc atcggtagcg gccagttcgt gaaactgcgc
    22501 ggataaattt atcgaagcgg ctaacaaaag cacaagcaaa aagcgcatag tgtaatttta
    22561 gaccggaaga tctgttgtta tcggggccct actcattccg caatacttcg agcggtttct
    22621 ggccgagaat acggtacccg gcgagccacc cggcgcttgt ggcaagtaag gccgtgaatg
    22681 caatagcaag aagattcggc accggatcaa agcggaagcc ggcatcgaaa aagcgctgta
    22741 ggagaagtgc cgagaaagcg gtggccagta tacttcccat caaccccgcg acggcaccga
    22801 gcacgatgaa ctcgatggaa aagatgccgg agacgcgccg ccgcgtagcg ccaagtgcct
    22861 tgaagatggc gatctcccgg acgcggcgaa accgcgtgcc tgccacactg gaagcgagca
    22921 caatggcgcc cgcgaggatc gcaaacgaag agacgaagcg caccacgatg gcgatctggt
    22981 cgacaacctc ctgaacaatt tcgagcacat ccgctgcatt tacgatggtg accgtcgggt
    23041 agcggcggta tgcagcccgc tggagcgctg cgacatcttt cgcacgcacc ctcatgccgc
    23101 cgtagtagat cgccggcaat ccttcgagcg cgccgggagt aaaaacaaat tcgacgctcg
    23161 atcctgggcg gaccgcctcc gtgcggtgga tactcgagac ctgtgcggaa aacgtccggc
    23221 cggacgcgct gaattcaaga cgcgccccgg gctggatgtg aagaacttca gccgcatctt
    23281 cggacaccga aacttgcggc gggcccggat ttcgcttatc ccaccattgc ccgctcacaa
    23341 tttcagtgtc ggaaggcttt tcttccgcgt gtgtgaccag gcgtgtccgg taaaaccggc
    23401 gtctccgggc gtcattcgaa gccttttcta ccggtacgtc gtcgatggat tccagcttgg
    23461 ccgaaacggc ggcaaccact tcgggaggct tctcaacacc cggctgtttg gaaagcagat
    23521 cgagcaatcc cgcacgctca cgctcggtga cgttgatcag aaaaacattg ggcatttccg
    23581 gaggcgcgct gcgaatcatc tgcaacagca tggagcgctg caccagaaaa acggtgagag
    23641 tgaacatgac ccccacccct agcgccgcca ggatgaaggg tgtttgtgta cccgggcggt
    23701 ataggttcgc cagtccatgg cgaaccgtcg ccggcagcgt gatgggaccg tgttccacta
    23761 cagctttcag gccggcgagc agaagcttcc cgaagccggc catcagcacg agactcacga
    23821 acagaccgcc ggcgaaccag ccgccgatac gagcggagta cgagagcgaa ccaccggcga
    23881 gcgtagctgc gagcacaccc aatccggcga gaatgacggc cccgcctacc gccgcgctac
    23941 gaagattccg gcggcgttcg gtccacgaaa gccgcgcttc gggcatatca cgccggagga
    24001 taagacccgg ccggatccgt ctgatgccgg ccaggggagg ccatgtgaaa agcagtgtgg
    24061 tcaggatacc cgcgctcatg ccttgcaaca acgtccagaa atcgattcgg atgccggggc
    24121 caatcgggaa gtaacgggag agcaaggcgg ggaaggcagc ctgcacaaga aaccccaccg
    24181 cgacaccggc gagcgagccg atcaggccca gcgaaagggt ttgaagcagg taaatccgga
    24241 tgatctgtgg cgatgccgca cccaggcatt tcatcacggc aatcgaatcc attcgctgcc
    24301 gcaaatggga atgtaccgcg gtggcgacac cgagagcgcc cacaatcaac gagatcaggc
    24361 ttaccaggct caggaacgtt gtagcgcgat tcagcccacg ggtaatcagc ggatgcgtct
    24421 ggcggaagtc ggcaataagc gtcgaaggaa agacacgttg caactccgcc cgtacggctt
    24481 ccacgcccgg ccccggcgat ggaagtttga acagaaagcg ctgcgaagcg cggctgcccg
    24541 gcaccatgat tttcgaacgg tccagtcctt cgcggctcag cattacacgc ggccccacgt
    24601 tcaggcttcc ggtcatgcga tccggctcca tcgtgacgat tgccgcgata cggtaatcgc
    24661 tttcaccaag acgaatcgtg tcgccgatcg atgcatccag ccgcagcaga agatccgccg
    24721 agacgacaac ggtgtccggc gtcagcttct cgcgcagcgt gccgccggtg ctcagccgga
    24781 tctgcccgta gaaggggtac aagacgggct cgaccgcttt caccgaaacc agcagaggat
    24841 ccggaagatt gggagtggac accatggaga ccgtttcggt gatccatgtc cggacgatcc
    24901 ctttccgttc gagcgccgtc atggcggccg attgcgccgt gtcgggcatc tcgaacatac
    24961 gaaccgacag gtccgccgcc ataagggttc tggcctcgcg gagaagcatt ccgcgaaatg
    25021 cttcgctgaa gccgcgcacg ccggtgatgg cgcccactcc cgcggcaacc gccagaatca
    25081 caaagacgaa tttgcctttg gcggcgcgcg cctcccgcca accgatacgg aaagcggtgc
    25141 gccaggggaa gcggttcata gctcgtccgc cgcgatcact ccgtcgcgca gcaggacgcg
    25201 ccggtcggct cgtgcgctga tctcctgatc gtgcgttaca aggacgagag tcgtctcttc
    25261 acgccggttc aggccgatca ggagatcgag aaccatgcgg ccattcgccg tgtcgagatt
    25321 gcccgtcggt tcgtccgcca tgatgatagg cggacgcaaa acgaacgcgc gagccagggc
    25381 gacacgctgc tgttcgccgc ctgaaagttg aaccggataa tgatcgcgcc ggtcgagcaa
    25441 gcccacttgt cccagcagtt cacgggcacg tttcacgccg gtatgatttt ccccggccag
    25501 ctccagcggc agcagcacgt tttcctcggc cgtcagcgtt gggatcaatt ggtaagactg
    25561 gaatacaaac ccgattttgc ggccgcggat ccgcgccagc tcatcttctt ccagcccggt
    25621 gatatcgttt ccgtcgatga cgacgcggcc ggaggttgcc gtgtcgagcc ccgcaagcaa
    25681 tcccaggagt gtgcttttgc cgcttccgga tggccccatg atggccacga actgcccgcg
    25741 tggaatctcc agatcgatac cccgaaggat ctccaccgtg tgcgcccccg aatcgatggt
    25801 cttggtgagt cctgatgcgt gaatcatcgg actatgtttc gacatgcagt cccattcacc
    25861 attatgatga gtcttgagtg agggttctga tttttctcgc cgccggtctg cttctggcgg
    25921 gttgccgcag cgatcgtgaa gccgcgcggc agccgccgcg ggatgccgct gattctgata
    25981 tgacttcacc cgccgataag cgcccggtga tcgttgcgtt tggagacagc ctgacggcgg
    26041 ggttgggcgt ggacgcagga aaaggctatc cggatttttt gcaggcgatg ctggacgcac
    26101 gcggatacgc ctaccgtgtt gtgaaccagg gcatcagcgg ggacaccacc agtggcggcg
    26161 tggagcggct ggatgcggtg ctggccatga agccggaaat cgtgatcctc gaattgggcg
    26221 caaacgacgg attgcgcggg cttccgctgg cgtctacacg tgcgaatctg gatgaaatga
    26281 tcgaaaagct aaaacagggc ggcgcccggg tgatcctggc gggaatgacg ctaccgcgca
    26341 actatggccc cggttatatt cgcgatttcg agcgcatctt caccgatctg gcaaagaagc
    26401 attcggcgcc gctgatcccg ttttttctgg ccggagtggc tgaatccggc gagcatatgc
    26461 aagccgaccg gcttcatccc acggcggaag gtaaccgggt agtggcggcg aatgtcatga
    26521 aaacactgga accctcgttg aaaaaaggtg aagccgggca agcgccgcat taaaatgacg
    26581 gaagaagggc tgcttctacg cgtagctgtt acgagcggcg gagccattgt acatatctgt
    26641 gagtagagat ctatccagtc ccggcaccga agcttatttg cggtgggaaa gtacgagcgg
    26701 atccgtatcg gtggagttga gccgggaact gatccagatg ctgcgaggtg aagtatatcg
    26761 cggcgtgcag gctcttccgc ggcgcggcgc cgaagtcggc ggccttctgc ttggacggtt
    26821 ctcgagctcc aatgagacct ccgtgcgcgt ggaatcgatc gaaccggttt cgtgcgagca
    26881 ccgcttcggc ccttcgttca tcctctccga taacgatcgc accggtctgg agcagatgct
    26941 cgccgcgtac gaatcagacg aagaagtggc agtggtgggc tactaccgaa gttgtacggg
    27001 acgagacatc gagctggatg aggccgacga agaactggct cggatgtatt tcaccgatcc
    27061 ccgccaggtt tttcttctta tcaaacctct atcgctgcgc gagtgcattg cagcgtattt
    27121 ttacaaggag cgtggacggt tcctaaccga gggcaagcca tcgttttcgc tcaatgccga
    27181 atacacgagc acacctacgg tacctgaaga accggcagtg cagccaagcg ctccgatacc
    27241 ccctgcggaa acgcgccact tcgagccgcc tatagtcgag ccgcctatgg tggtacccga
    27301 tacggtgcgc caccgtcgtc gcgcgcgccg cgaggaagga cgtgaggaag aagaaaagca
    27361 acccggccgc ctccggcgcc tgctgttgac ctttgtggct tttgtcgcga tggttggtct
    27421 ggcgttcttc ctgggttggt tgaatcctgc acgtgagccg cgcttcagcg acctcggcct
    27481 gaacgtacac cagacctcga acggcctgga agtggtgtgg gatggcgcaa aagccgcgtc
    27541 gcaggatgcc cggcgcggtg ttttatccat caccgatggg gcgtccgaaa atgcagtcga
    27601 gctgaaccgg gcggcattgg aacgcgggaa gttcgtttac gaacccgcca gcaacgacgt
    27661 gttggtgcgg ctctcaattt acggagcggc cggccagcct gcgggtgagt cttttcgtgt
    27721 cgtagtggtg ccgcagaaga cgccgcctcc ggccaaagca gcgccggcca tcgagccgcc
    27781 gccacagatt gcgcccacgc ctccgtctgt gaacgcggcc aggcacgtca aagcagaagc
    27841 gcctcgcata cccccgccgc cggctgcaaa acccgcgcgc cagcagacgc ctgtcgcatc
    27901 tgctatcgcc gttccggcca aggcgattca tgaagtccgg ccggggattt cagaaggtat
    27961 ccgctcgcga atccggggca cggtggtcat cgcggtggaa gtccgcattg acgctaccgg
    28021 taaagtcacc agcgccgtac cgcagggaag gggcgatgtg gtttacacct atcttgcttc
    28081 ccgagccgaa gaagcggcca agctatggcg tttcgagccg gcccgcacaa aagacggcac
    28141 ccgcatagcc agcacgaaaa ccgtctattt tgtttttaag ggctgagcgg ttcacttcag
    28201 cgtgcgcacc ttgatagacg agagcgattg gcccgaacga tagacgcgtt gcgttgcctt
    28261 catgaaatcc gcctctgttg cttcgtgaat cgggacgaat ttctgtgggt tgcggtccac
    28321 cagcggaaac caggagcttt gaatgtgaac cgcgattcga tggcctcgcc ggaaggtgtg
    28381 atagacgtcg ggcatcgtga acgcaacctt ggtcacacgg ccgggctcga acgcttccgg
    28441 tttttcgaag ctgttgcgga acctgccccg gaagggctcc ccgcgcacca gttgctgata
    28501 gccgcccatc ttcacacgtg tgggattcgg ctccgggtcg ggaaaagtgc cagggtaaat
    28561 atccaccagc ttcaccaccc agtctgaatc cgtgccggtg gtggagacca gcaaattgca
    28621 ttcaatgggt ccggcaacgg tgaaatcctc ctccagttcg cccgtctgat acaccagcac
    28681 gtcggtccgc cgcgccgcaa atctctggtc ggaggtcatg taggcggagg ccatacccgg
    28741 ggcgatctcg tccgtgaaag gcaccggttt cgaagggtcg ctcacgtatt catcgaacgc
    28801 gttttgagcg gtattcccgg gcgctttgag ggaaagcttt ccgccggcgt cgaaatagaa
    28861 cgtttgctcc gccgcctggc ccggaggcca gctatcgtgc ttccgccact gattccgccc
    28921 ggtttcgaaa acgtaggctt ccggcaactt cggatcttcc ttgtccttca ggtggcgcaa
    28981 gaaaaaggga aacatcatct gttcgcgaaa gaatctgccg gtctccgaag caaatctggc
    29041 attgcccaga cggtctcccc tacccatcca gccgccgtgc gaccacggcc cgataaccag
    29101 catgttgttc gcgccgggac tctgcttttc agcggcctgg tagacggcga gcggcccgaa
    29161 cacgtcctcc gcatcgaacc agccgcccac tgtcatcatc gccggcttga tgttcttgag
    29221 atgcgggcgg atgttgcggg cttgccagaa agaatcgtag cgatcgtgct ccatgagttg
    29281 tttccaggag gcctcttcgc ggccgaagag cgttgtgcgg atgtttcgaa gcgggccgag
    29341 gcgcaggaag tactcgtagc cgtcgggcgt tcccaggtcg aaccggcgcg ggggcacgct
    29401 ggtgcccggc tctatccaag gctggccgaa gaagatgaaa aaatcgaacg cgtgcggaag
    29461 gaagagcgca ccgttgtgcc gccagtcgtc cccgatgaac cagtccgcga tgggtgcttg
    29521 cggggacacg gcctttagtg ccggatgcgc gtcaatagcc gacatcgcgg cgtagaagcc
    29581 ggggtaggaa attccccaga caccagcctt gccgttattg ttgggaacgt tcttgacgag
    29641 ccagtcgatg gtgtcataag cgtcggtggt ttcatcgatg tccttaggcc cgcgcttcac
    29701 cgtgagatag ggccgcatat tcacgaactc gccttccgac ctcgtgcgcc cgcgcacgtc
    29761 ctgataagcg aagatgaagc cttcgcgcat gaatttatcc aggtcttcgc caatacctct
    29821 gtagttgtcc gagccgtagg gagagatcgt atacggcgtc cgctggagaa ggatcggata
    29881 gggaacggaa gtatccttcg gaacataaac tgccgtgaac agccgtaccc catcgcgcat
    29941 ggggatgagg tgctcgtact tggtgtagtg ggccaggaag taatcgcggc gctcctctgc
    30001 tgctttgcgc tcaggcgaag acggagcctg cgagtacaac ggagcctgga gacaggctgc
    30061 gacggcgacg gcggcgagta gttcgacaaa acgtttcatg gggacatgaa atatattacg
    30121 caaaagcttc gcgcaaaact ttgaggctct tgggtacggc cgtcgaggca ggttcctttc
    30181 cctccatttc gagcgatacc caaccgcgat atcccgcctt gcgcagaatg ccggcgatgc
    30241 gcttgtaatc gagatcgagc gtgtaccatt caccgccgcc atagtacgtc tttgcctgca
    30301 cgatggaggc ctctggagcg agttgcgcga ttccggcgta agggtcgccg gcgtagtttc
    30361 ctgtgtccag attgatgccc agccagggcg aattaatcgc gcggtagatg tgcagcaggg
    30421 tctcgggctc tgttgtcaga ccccaatgat tttccagcgc gagcaataca cccgccttct
    30481 ccgcgtgagg caggcattgc tggatggaat cgatgcacca ccgcacggca tcttcgttcg
    30541 tgtagccgtc aagcggcgac tcctggcctt tcactttcat aaggtcatcg aaagacttga
    30601 tggttttcca gcgtccggag ttcaggcgga tcgccgggat tcccagttgc gccgcaatgt
    30661 cgatgcagcg tttggtatgc gcaatgtgct tctgccgctc tgccgcatcc ggccagacga
    30721 agtcctggtg tatggatagc atcacaagcg ccagcccgtt tcgaaaagcc cgcttcttca
    30781 ggtcgttcag ataggacgtg gtttcatcgc gcatctggcg atgcagaatc tcgaccccgt
    30841 cgaacccgag ccgcgcggca tcgtcgatca cctgctcgat gggatacttc tcggttcgga
    30901 agtgccaata agagtaggtg gagactgcca gccgtatacg gtcacccgga gattgggcgg
    30961 gactctgccc ggacgctccg aatagcgggg ccgcagcggc cggaattaac acgtctcttc
    31021 gtgacattcg catgaatggc ctctcgcctg tctcatcatc tcaggaaact atgggaacat
    31081 ggtcccaaga ggcggcattg caggttctcc gaatccgtgc gcgtacggtg ctaacgcttg
    31141 ttctttgcct tgccggtgga tggctgtccg gttcttcgat gagtgcgcag caggcggtca
    31201 cttccgctac gctgggcggc cgggttgagg atgcgagcgc cggtgttctc ccgtccgttc
    31261 tggtcacggt agacgacacc cttcgtggcc ggaatttcgc cgccaggacg gactccggcg
    31321 ggcggtacca gtttctgcgt cttgccccgg cggactacat tatccgcgtc gaggacccgc
    31381 gctttgaaaa atacgagcgc cgcttgccgc tgttggcggg acaagccgtc gacctcccga
    31441 ttgtgcttcg tgtcgcgggc gttggccagt cgcttgccgt tatcgcggag gctcctgtgc
    31501 tcgaatcggt tcggacccaa atcggtgaga ccatcggtgc tcacgatatc ggcgggctgc
    31561 cgctaaacgg gcgcaacttt ctcgacctgg ccttgctggc gcccggggtt tcccgcacga
    31621 acaccggtac gccgcagcag ttcgccgaaa cgtccgctgt gccgggaacg ggaatctcca
    31681 tcgccggaca gagaaatctg aataacgcat tcattgtgga cggtctctcg gccaatgacg
    31741 acgccgccgg tctcgccggc agcttttaca gccaggaggt cattcagcag ttccaggttg
    31801 tcacttcggg cggtaatgcc gagtttggac gcgcctccag cggagtggtc aacatcatca
    31861 cgaaatcggg taccaaccag tggcacggaa gcgcatacgg gtttctccgg aaccagcggt
    31921 tcgatgcgcg aaatcctctt gcaacacgga aggaccctct aacccaaacg cagtacggcg
    31981 ccagcgccgg cggaccgctc atacgcgggc gcactttcct gttttcaaac ttcgagcagg
    32041 cgcatcggaa tgcggcgggg ttcatcacca tcgcaccgtc caatgttcaa gctgtcaatg
    32101 ccttgctcga acgtgtggca taccgcggcc ccacgcttcg aaccggggag tatgcaaccg
    32161 gctgggattc ggtgaactat ttcgcgcgcg tcgaccatca actcacctcg tcgaaccagt
    32221 tgacggtgcg atacagcctc tatgacatcg ccagtccgaa tgcgcgttcg gtagggggtc
    32281 tgaatgcgga gagccgcgga acccggctcg acaaccgcga ccagacggta gccctgagcg
    32341 atatggctgc gctgtcttcg cgggacctga acgaactgcg cgtgcaattc acccgaagcc
    32401 ggctctcggc gcccggtaat gatttcatcg gaccggcggt gaatatcgcg ggcatcgcta
    32461 atttcggcgc gtccaccacg tctcccaccg cgcgggacaa cgacctgtat gaagtgagcg
    32521 attccatctc catacagcgc ggcgcacatt tgttcaaggc cggcgccgat tttctttaca
    32581 accgtctggc gatcgatttt cccggaagcc ttgtggcggc cgtatactcg tttccatcgc
    32641 tggccgcctt cgatgcgggg cgatattcga cgtttcaaca agcctttgga gagccccgcc
    32701 agcggcagcg caatccgaat atgggatggt ttgcgcagga ggaatggaaa cccgccgcaa
    32761 actgggccat caatgccggt gtgcgctacg atttgcaatt cctgccttcc ccgattcaac
    32821 tggatcgcaa taatatcgct ccgcgaatcg gcatcgcctg gtcgccgccg gaccgcaaga
    32881 ccgttgtgcg ggcaagctac ggcctctact acgaccgcgt accgttgcgg gccacttcga
    32941 atgccttgca gcgcgacgga agccgttacc gtgtggcact tctggccttc ggccaggacg
    33001 gcgctccctt attcccggcc cagttgaatg cttttcccgc cgggcagttt atcaacatca
    33061 caaccatcga tcccaaaatc cagaacagtt atagccatca ggcaggcttg caggtggaaa
    33121 gagagctggc gcatgcgacc gttttgtctg tgggctatca gtgggtacgc gccctgcacc
    33181 tgatcctgtc gcggaatgcg aacgtgccca ccttgtccgc ggcggacgca gccgcgttgg
    33241 gagtcccgaa tcttggccgc ccggattcga ggtatgggaa tatcggccgc tatgagggtg
    33301 ccggcgattc gtatttcaac ggcctgttcc tgagcctccg ctcgcgcccg tggaaaggga
    33361 gcgaactgcg tgtatcgtac acgctgtcca aaaccatcga tgatgtaggc aatttctttt
    33421 tcagcacgcc gcagaacaat ttcgatctgc gggacgatcg cgggctctcg gacaacgacc
    33481 agcggcaccg gctttccgtc gcggcggtgc tcgattcgcc cgtgcggtcc ggaccagggc
    33541 ccggccggta cctgctttcc ggctggcggt tgtcgccatt gttcctgtac acatcggccc
    33601 tcccgtttaa cgcgcaggtc aatttcgatc gcaaccagga cacgaacctg aatgacaggc
    33661 cgttcgggat ggggcggaat acggggcggg gattcgacta tgcatccctc agcttgcggc
    33721 tcgcgcgatc gttttctatc accgagcgcg tgcgcggcga agctctcgcg gaaggtttta
    33781 acgtgctcaa ccgggtgaat cgcgcgctgc ccaacaacat catcggttcc ggtacggggc
    33841 cgccgttgcc ttcgttcgga agagcgactg ctgtacttga tccaaggcag atccagcttg
    33901 gaatgcggtt tgatttttag gtttgagaat gcttaccgtt cttgcgtttt tcacgttgta
    33961 cctgaatccc gcgtcagcgg aggttcccct ccgtcagccg caactcgccg ccgatggaca
    34021 cacggcggtg ctcgcctatg gcgccggtaa atcgatcttt gtagcagtat cttccgatca
    34081 gggcgctact ttttcgaaac ccgtcaaagt ggcggaagca gaggttcttc cactcggcag
    34141 gcatcggggg ccacgcgtcg ccgtcagcgg ctctacgatc gttgtcacgg cggtagtggg
    34201 gaaacgtcgg gcggaggggc ctcacgcgca cggcttgccc ggcgatggag atttgatggc
    34261 atggtactcc tccgaccgtg gtgcaacgtg gtccgccggc aggccggtga acgatgtccc
    34321 gtcagcggca cgcgaaggat tacacactct ggcgggcgat ggacgcgggc gcgtgtttgc
    34381 ggcgtggctg gatctgcgta ggcagggaac ccgcttgtat ggggcgtttt cgaaggatgg
    34441 aggcgctacg tggagccgca atgtgctggt ctacgagtct cccggtggaa ccatctgcga
    34501 atgctgccac ccgagcgcga gtttttctcc ttcgggttcg ctttgggtca tgtggcgtaa
    34561 ttcgctcgaa ggctcgcggg acttatacgc gactcactct aatgacttca cccacttcag
    34621 ccctgcgcag aagctgggaa acgggacatg gcttttgaaa gcctgtccga tggatggcgg
    34681 cggaatagcg gccggcggga accaggttgt gacggcgtgg cgccgcgaat cggacatcta
    34741 tcgggtggaa ccaggaaaac cagagacgat gttgggcgcg ggcaaagatc ccgctatcgc
    34801 gcaaagtgga gaccagacct tcatcgtatg gaacgaaggc ggtggcatcg tactctggag
    34861 caacggcggc agtattcgca agttatcgca gaagggcgct tttccttcga tcaccggctt
    34921 accgggcgca agcgccgtta cagcctggga gcaagacggc ggcatcgcgg tggaggttgt
    34981 acactccggc ggccgccatt gaccggcgaa tgagcttgtc agggccccac cgcggtccgc
    35041 cactcggcaa gggctttggg aaggaagctg gtgagtgttt catcgcgatc aagagcggca
    35101 cgcaaaacgg gccggatcac aagggcaacc gcccacgcct ggacgtaggc gtccggccag
    35161 gctttttccc tatttcgtag agctcgcgcc aacgattgag cgattgtatc ggattccagg
    35221 atattgtccg taaggcccgg accgaatgca tatgcgatgc cttcgttcag agctccgaat
    35281 cggattccag cagattgcgc cgcggcctcg atcgtttctt tgtgccgcca aaggagagca
    35341 tgaaagcact cgtgaaaaag cgtggggacc ggatcgggac ggtcctgtat ttcgagcacc
    35401 agccgcccac cgttgaaccc gcccccggca ttcccttcct cggggttaat gattaagaaa
    35461 agcggcagtt ttacggtatc ttcgatttct gaaaaccgga cgagcttttg cactgtaggc
    35521 gcaagcctct tcgcttcggc tgtcaaacga tccttgaaac gagctatttg aggggcgtca
    35581 cgcaaaacgg acagcttcgg agcgaagtgc aacaagatca acttctccgc ggctgcctca
    35641 tcccgcgaga gaagtccctt ttccacggct ttgtccgcag ccacttcaat cgggtcatcc
    35701 acgtagaagg actcttcgaa ccccttgttc cagccgcgtg cacggcgaag cacgccatgc
    35761 ttccggagca actcgcggtc ttcgccgtcc agggtaagcg ctttagccgc ccaccgccca
    35821 tactgccgat ggcaggcgtc atcccactcc gagagctgat ccacgatgtg gaacacctgc
    35881 gccgtccagg aatccgatac cgaaaggaca atacccccga tatccacctc ggcagtttgt
    35941 gcgccgcctg caagggcggt ggccagaaaa cagatacaga gcagcaaaag cctgaaatgt
    36001 ttcggcatcg ttaatccccc gcgcattgcg ctacgggttt ccggccttcg agaggatcgc
    36061 ttacgcgcac ctgctttcgc ttgcgggatt gccccatcgt gatcaagacc accgcggcaa
    36121 cgatggtgca cattgccagt accgacgagc gcgttagcgg ttctccggca agagcccatc
    36181 cgagcagcac cgcgaccacg ggattcacga atgcatacgt gctcgctttg gccggcgtgg
    36241 tgtgagcgag gaggtacatg taggaagtaa aaccgatcag cgatccaaag atggccagat
    36301 aaaagaatgc ggcgagcgac gccatcgata tcgtgtccgg gcgcaagtgg cttacttcgc
    36361 cggttagaac tccggttata gacagcagcg cgcctccgca gagcatcgtc atggcgttcg
    36421 caagaccgga gaaacgtgac gtcggggcag tggtcccata cagcgacccg gcggcccagg
    36481 aaatcgcact caccagaatg agcgctgccg aaaacaggtg aaacccgccc gcattatcca
    36541 cactccacgc tggagcaaac agcagggcta caccgccgaa accgaggccg atagcagcca
    36601 ggtccacctt gcttggggcg atgcctcccg gccacgccca tgcgagcaga accagccaca
    36661 gaggctcgct ggcaatcaac aaggccgcca caccggacac gatgcgctgc tcggcccagg
    36721 tgacggcgcc gtttcccacc aggagcatga atgcgcccat tatgagggcc gttcgccagt
    36781 gaatccactt tggccgctcc gctccccgcc aaatgctcca ggccagcagc acggaaccgg
    36841 agagcaaaaa cctgctgcct gccatcagga atggcgggat ggtttcgatc gcgtagcgga
    36901 tcgcaagata cgtggagccc caaacaatgt acaccgccgc gaacgccgcc agcagtttcc
    36961 attgcgcatg tttcgcttca ggcatgggca cgcacctcgc ggtccgccgg gacgggaacc
    37021 gcaagggtct ccttggccgt ggtaagaaca atgctggtac gggtggagcg tatagtcgga
    37081 atagcgccca ggcgatcccg caacagcacg ccaagcgctt ccgtatcgcg cacccgcacc
    37141 ttcaccagaa aacagtcctc cccggcaata tgatgcactt cctggatctc cggaatcgca
    37201 ataagcttct cttccgtttc gcgcgaaccc agcggctctt ccgcccgtac aaacacgaaa
    37261 gccaccaacc cccagccgag cgccatggcg ttcaaccgtg cctggtagcc ctgaatcaag
    37321 cgccgttcct ccagcttacg gacgcgctca aggaccgctg agggagccat acccaactgg
    37381 cgagccaact ccgcgttgga tatccgggca tcctcctgga gaatcttcaa aatcgtcaga
    37441 tcaacgttgt tcattcagtc aaaaaataaa ttatacgacg tttattctgg caatgtaaat
    37501 aaaaaaagaa taatcttcac tccagatcgt cccacgggcg aaagatgtgc tgatgctccc
    37561 gcatgtcgac ccgagacccg cgaaaccgga cgccctccat ttccaaccgt atacgctggt
    37621 cagaggccgc ctcccctttg agccggattt gcccgccggc gcccaggacg cgttgccacg
    37681 gcagcgaccc gctcgatttc cgtaaaatct ggaccacctg ccgatgatgc agaggatagc
    37741 ctgcggccgc ggccacttgc gcatacgtcg cgaccttgcc gcgaggaatc tgccggatga
    37801 tacggcgaat gaccctggtc atatcctcgg aagcgcgaag ccgcagcagt cttcggctaa
    37861 gttcttttgg attcttccca gccattccgt aattatgctg gaacgcgcgg cacttcagcc
    37921 gttcgaaaga actttgagtc cggcgatgcc gccgatgatt aacatcaggc agatcaaacg
    37981 caacggattc gcggattcgc ccaggaccag tattccgaat agcgcggcgc cgacggtgcc
    38041 gatgcccgtc cacacagcat aagcggttcc gataggcagc gtacgaaccg cgatgcccag
    38101 caggcccatg ctcgcaatca tcgccagaac ggttagaatc gtgggcccca agcgcgaaaa
    38161 cccttcggtg tacttcagcc ccacggccca gcccacttcc aggattccgg caatcacgag
    38221 aatcgtccac gccattttct gatagttctc ctcttctcaa gttgaatggt cctgcaaaga
    38281 cctcatgtta ttctgatagc tgaacctaca ttccctcaag ttggaccaac acttttgaag
    38341 atcggttttg taagtctggg ttgtccgaag aacctcgtcg acacggaagt catgatgggg
    38401 cagcttgccg cacggggaca cgaactcagc agcgacccgg cggaagctga ggccattgtc
    38461 gtgaatacgt gcagcttcat cgaccccgcc aagcaggagt ccgtggagac gattctcgag
    38521 atggccgggc acaagaagac ggggcgcctg cggcggttaa ttgtggccgg ctgtttggtg
    38581 gagcgctatc gcgacgacat ccgcaaagac atcccggaag tagacgccgt catcggaacg
    38641 aacgagttgg agggcattgt cgctgcttgc gaagcgggtg agtccggaca taacgccgga
    38701 gcttcgtacc tgtaccacga tctgacgccc agaatcctgg cgactccgcg gcactacgcg
    38761 tatatcaaaa tcgccgaggg atgcgaccac ccctgttcgt tctgcgtgat tccgcagttc
    38821 cgcggccggt tccgcagccg ccggttcgaa tcggtggtgg ctgaggccac gcggctgttc
    38881 gcgcggggtg tgcgggagat caatctgatc ggacaggata caacctgcta cggcgaggat
    38941 ctcggcatca aagacggcct ggccgttctg ctggagaggc tggccgggat tgagaccagc
    39001 ggccggaaat ggatccgttt tctctactgc tatcccaacc gcatttcaca aaaattgctg
    39061 gacaccatcg ccgcgcattc cgcgctggtg aagtacatcg atgtcccgct gcagcatgcc
    39121 agcgcgaatg tgctgaagcg catgaagcgg ggcggctccg cggagatttt tctgcggctg
    39181 atcgagaaga tccgccggac gataccggca gtggccatcc gcaccagtat gatcgccgga
    39241 tttcccggcg agacgcagtc cgatttcgag gagctttgca atttcgtgca ggccgcccaa
    39301 ttcgacaggc tgggcgtttt ctcgtactcg gatgaagaga ccagcgcgag ctatcgtctc
    39361 tatggcaaag tcgacagccg cacaatctac cagcgcaagc ggcgcctgat gagtttgcag
    39421 aagaagatct cgcgccggcg caacggcgct cttctcggcg aggcagtgcc tgttctggtg
    39481 gagggcgcct cttcggaatc cgagtttttg tggcaggcgc ggcttgccac gcaagctccg
    39541 gaaatcgacg gtgtctgcta cataacggat ttgggcgatg ccccgccggt tcccggtgaa
    39601 atacggcaaa tgcggattac ccgcgcgcac gactacgatc tggaaggggt gcttatcgat
    39661 agcgccaatg aagccttgcc tgtgccgcgg gccgagttgt ttccgattct ggccgcgccg
    39721 cgcgcgcgta tcgttcccca aagcctatga agtgcccgat ctgcaaaaag ccggttgcga
    39781 tagacgatcc ttacatgcca ttttgcagcg aacgatgtaa attgatcgac cttggcaact
    39841 gggcgtccga aaagtatgta atttcctcac ctgttgttga gccgcctccc aagtccggcg
    39901 atgagtaagc gcgcgggccc ggtggccgtt gcactggcaa catggtttgg atgcgggttt
    39961 gctcccgtgg cgcccggcac cgcgggatcc ctgggcgcca ttgctgttgc gatatcgctg
    40021 gaccgctggc ttcacccggc ggcatactgg tgggccatcg ccgcggttct gctttcgata
    40081 ccggcgattt gggcggcgga ccgcactgcc gcgggagtgg aaaagaagga ccccgggctg
    40141 gttgtggtgg atgaggttgt cggccaatgg attactctcg ccgcgctgcc gtcgctccat
    40201 tgggctggcg tgaccgccgc attcctcctg ttccggttgc tggatattgt gaaaccgtgg
    40261 cccgcgcggc gccttgaatc gctgccggga gggttcggta ttgtcgccga tgatgcaatg
    40321 gcgggcctgt atggcgcgct tgtgttattg gctgcgggat gtttcaatct ttattaacaa
    40381 ctatggcgat gcgcaaagat tccgacggaa aaccgcagat ttccgcggtc catccggcgg
    40441 cagcgattcc tgggggtgaa ctacagatac ggggcaaggg gtttgccaag tcaacccggc
    40501 cgcaggtcac gatcggagag ctagaagccc ctatcctggt cggttctgat tcctatgttg
    40561 tcgccaaggt tccggaaggc acttccgaag gtgaattggt agtgatgaac ggagagaagt
    40621 cgagtgttgc ctgggcgtgc gatatcggga tccagatcgc cgacagcgtg cacccggtcg
    40681 ccaacccggt tgtcgatccg gccggtaaca tttatacgac tctcagcgga cctcgcggac
    40741 agaagacgcc tgtcgcggtc tatcgtatcg acaccagtta caacatcaaa ccctttatta
    40801 cggatctgat gaacgccacc ggcctggcgt tggacaatga aggtacgttg tacatctcca
    40861 gccgtcatga cggcatcgtg tatcaatcct cgccgtcggg cagcctgtcc gtttacgtgg
    40921 aaggaatggg ggtggcaacc ggtctggctt tcgatgccga cgggaacctc tatgtaggcg
    40981 accgaagcgg gactgtattt aagatcagcc gcaaccggca aatctacgtc tttgccacgg
    41041 tcgaggcttc catctccgcc tatcacctca cattcggacc cgacggatac ttgtatctcg
    41101 ccggacctac cacatcgagc tccgacgcgg ttcaccggat ctcgtcggcc ggggaagtgg
    41161 aagtgttcta caagggactg gggcgtccgc aaggcttggc gttcgatgcc gacgggcgcc
    41221 tatacgtcgc cgcatccctg ggcggccggc gcggcatcgt tcgtatcgat gccgagcgca
    41281 acgcggaact ctttctgtct ggtccgggta tcgtcgggtt ggcattcgcc ccttccaggg
    41341 cactggttgt cgcaacgaac aacgctctgt tccgtgtgga gaccggtatt aagggtcgac
    41401 cgttgccttg atgcccctcc agccgatgca agccgagatc atcgccgttg gttccgaact
    41461 attgaccccg cagaaaatcg atacggattc gctttatctc acgggcgagc tgaacgcctt
    41521 gggaattgaa gtggtcgcca aaaccgttgt gggagacgac cgtgaacggc tgtccacaat
    41581 gatccgcgat tccctccggc gttcgccgat cgtgttcctt accggtggat tggggcccac
    41641 cgaggatgat gtgacgcgcg atgcagtggc cggtgccctc gggcgggaac tgcaattcaa
    41701 tcctctcgtc tgcgagggaa ttgaggcgcg ctttcgcctc atgaaccgcc gcatggcgga
    41761 agtaaatagg cggcaggcgt atatcatcgc cggcgccgaa atcctgagca acgagaacgg
    41821 taccgcgccc gggcaatggg tcgatgataa cggacggatc gtgattctgt tgcccggtcc
    41881 gccgaacgaa ttgaaaccga tgtttgcccg ctgctgtttg ccgaaacttc aggcggcgat
    41941 accgagggcc gtgatccgga cgcggttcta cagggtggcg gggatgcctg agtccgagct
    42001 ggatcagttg atctcgcccg tctatacgcg ctacagcaat ccggtcacta ccatactcgc
    42061 cgctcctggc gatattcaga tccacctgcg ggcgcgaacg gaaacggagc cgcaggccga
    42121 agctctcctg aacgaactgg gcgcacagat tgaggaactg ctgggcaggc ggatttactc
    42181 gtgcaatggc gagcctctgg aggcggtagt gggtgtggcg ttacggcgcc ggggggaaac
    42241 gttgagtgtg gcggaaagct gcacgggagg ccttctggcg gggcgcatca ccagcgtcgc
    42301 gggggcgtcc gattacttcg ccggcggctt caccacctac acagaggcga tgaaatcatc
    42361 ggccctctcg attgatcccg aactgctgcg cgtccacacc gcggtgagcc aacctgtggc
    42421 cgaagccatg gctttggcct gccgcgaacg aacaggctct acgtatgcac tttccgtcac
    42481 cggcgttgcg ggtccgggtg gcggaacaga acagaacccc gttggcaccg tgtacatcgg
    42541 actcgccggc gatgaaggat gcgatgtcag gagaatgcgc ttcctcggcg accgcacgcg
    42601 cgtccgcata ctcgccgtgc aaagcgcgtt gaacctgtta cgtctgcatc tcgatggcga
    42661 aagtggaagg tagccggtgc gtgccggaat cgcgtctcta ttgctcgtcg gctgtgtatt
    42721 agcgatagca tgtgccggcc gcagggagca gcgtgcccct tccctgcccg atatccaacg
    42781 ccaatcggtc acgggtgctg tgattccagc gggaacgatc atcgttctgc gaatcgtaga
    42841 tccgattgca accgccaagg cggacgactc ccttggctat gcggcggtca ccgcccggga
    42901 cattcccgag caatccggaa aagtgctgat cctcgcaggc tctccggtta ccctcggagt
    42961 ggcacaaaac agcagcggcc tcaaacttgt tttgcactcc gtgatgatca acgggaacaa
    43021 ctatcccgta gccggcaaag gtgcgcctct cggcgcggtg gtgcaagcac tggcacccgg
    43081 cgtggaaagt gcgcccccgg atcctgttgc agatggcgat atcgccgtcg aaggcgcaag
    43141 catgaacgta ccccggggat cactgctgac cttccaactc gagcagccct tcacgattgg
    43201 cgagggttcc ggctaaccag tgagctccta tccgggttcg cggattgcag cgggcgaatt
    43261 gcgcaccgca cgccaggctg gcgggataca ggcgagacac gaaatcgcaa accagattgc
    43321 gattcccgcc atcaacacgg ccggttccac cgcccgcaac ccgtagagca tccgcgccag
    43381 cgcaacgttc gccacagctc cgagcggcag tcccaacacg ataccccaaa gcgtcatgct
    43441 gagaccttgt atgaggacca gtctcagcac atccacgggg cgggcgccga gggccgtgcg
    43501 tatcgccatt tcacgttggc ggcgcgatat cgagtaggcg atcaccccgt acagtcccag
    43561 aaccgccagc accaccgcca ccccgcaagc ccaggcgacg acccgagcgc cggtgcggac
    43621 cggccaaagc acatcgctca cctgcatctc cagagtgcgc atccggcgta gcgagatagt
    43681 atcgtccacc gattccacca cccggcgtac tggccccatc aaatcctcgg gcgccggaac
    43741 cgtccgtatc accaggtaca gcatcgacga gtaccgttgc gtggccggaa gaaacagcat
    43801 cggctgcggc gcttcggtaa gcgatcaccg gaagtcgctc gccacgatgc cgacgatctg
    43861 caccgggctc ttctccttgc ccactcggag cgtccggccc agtgcttcga gcggcgctgc
    43921 ggcatcgggc cacaaccgcc ggacgaggcg ctcatccgcc agcgcgaccg gctgtgagcc
    43981 ctctcgatcg tggctcgtga agttgcgccc gccgacaaac cgcaccttca tggtcgtcag
    44041 aaactctgag tcgatcaggt ccaacccgcc cggaccggcg cgcctgcatc gcgccagact
    44101 tcgatctgcg atgcctgcgc gttcagaggc acatagctgg cgaggcccgc cgattccaca
    44161 cctgcgagcg agcttactct ttcctttacc gaatcaaaga accgccggct cttctcctga
    44221 ttcccggcat aggaggccag gttcagattg agcggcacga tgaggcccct cgtgatagca
    44281 aaacccgggc cggtctcctg ttggctcgca aaactgcggt agaacaggcc ggctgtcacg
    44341 agcaggacca ccgaggaggc tacctggcat gaaaccaaaa ctttgcgggc gaaccagcgt
    44401 ggacggccct gcgcgtcctg gttgcggatt gccggcaggc aatctgcccg cgccgcctgc
    44461 aatgccggaa ggatgccaaa tagcagcccg cagaacagac tgatcgccgc cgtgaatatc
    44521 cacacgcgaa aatcctggtg gatggctacc cggtatgtgt agaggtcgtt ggccggcggt
    44581 tgcagttccg gcagcagccc ggtaatccag atcgagagga gcaatcccac ggtccctccc
    44641 aacaccgcca gcatcaggct ctcggtcagc gacaaccgga tgaggcggta gtcgcccgcg
    44701 ccgagtgcgc ggcgcatcgc cgtttcctgc ttccgcgctg tcgcccgcgc gacgagcatg
    44761 ttcgccacgt tggcgcagga gatcagtagc accagggccg ccaccgctcc gagcaggccc
    44821 acgaccaact ccacgcgcct gcgaatcaac gggattccga ttccgcgcgt caccgacggc
    44881 cacagccggc gttcttgtct ctccgtgtag cggtgctcct gctgaagttc acggtcgatc
    44941 gcgttcaggt ccgcaatcgc ctgggcctcg gaaacgcccg gcttcagccg ccccgcaata
    45001 gccagccagc gagcgttccg gtccgagaaa agttccggat tcgtcagccg ccggttctcg
    45061 acgtgaatcc agatatctgt catcaccggc gccagtgttc cgtcgaagcc ctcgggagct
    45121 atgccggtga cggttgcggg gacaccactc aaggccacct ggtgtccgat gacgctggga
    45181 tccccgccgt acacccgccg ccagaaacgg tggctgagaa tcacctgctg ggcacaggtc
    45241 gacgggcagt cgccggaatg aaacaaccgg cccaggaatg gcctgatttg cagcatttca
    45301 aagtagttct ccgccaccgt ttcagccatc acgctctggc tttgatcgcc gcgccccagg
    45361 aacaccggca tgatgttcca ggcggtgaaa tgcgtgaagg tccggttccg gtcgcggtag
    45421 tcgatgtagt cgggccagga ggatggctcg ctggtcccgt ccccgcgcag gacgcggacg
    45481 aacgtcgaag ggtccttgag cggcgtttgt acgagcagcg aattggcgag cgtgaatacc
    45541 agcgacgcgc cggcgatccc gccccccagc gatagcacta gggccagcgt gaacagaggc
    45601 gatcgcttca acgcgcgtgc gctggcgcgg aggtcccgcc gagttacttc cagttgttcc
    45661 cggggcatgc tgaccagcca atccagaaac gtcccggccc acgcggccgc ctgttcccgg
    45721 cgtgtgcgat gggccatccg ggcgctgaaa tcctgctcca tttcgtctcc gaactccgcc
    45781 cggaactcgg ccggatagaa cagcagcaga cgcttgtgga gccaggcgga aaaccggctg
    45841 agcatcatgt ggaggcgctc ctcggcagca gttttttcat cctgcccacg cccaccacct
    45901 gctccaatcg cttcagttcg gcttgcagaa cacgccggcc caattttgtc agcctgtaat
    45961 agcggcgccg cgaatcatca agatcgggag cggggcgctc gcccgactcc tccgccagtc
    46021 cgctggacag caggcgcttc agggtgccgt acagagtccc tgagcccgga cggaaactac
    46081 caccggtgcg gttcgcgatt tcgagcatga tcccatatcc atgccgctct tcgtccgcga
    46141 gagccagcag gatgtgcagg accacagggg tgaggggcag ataatcggtc gggtcctctc
    46201 gatgaaatgc cataatatgt ccgttacaaa tatgtccgtc agcgacatat tagtcgacac
    46261 cgatgcccgc gtcaagggat tttgcgaatg cgcgctgttg cccggccttt aagagattcc
    46321 cgggaaccct gggagttcga aagcactttc gggcggggtg acaagaccgc ccatgcggat
    46381 cgtgtccgta tgggagatta tccacctgga gctactcgga ttaggaatgc ctcgccggaa
    46441 gattcgggcc gatgggttac actag
//
LOCUS       DASTMK010000584        23517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_349364, whole genome shotgun sequence.
ACCESSION   DASTMK010000584 DASTMK010000000
VERSION     DASTMK010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 23517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23517
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349364"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..265
                     /locus_tag="VFQ79_24005"
     tRNA            190..265
                     /locus_tag="VFQ79_24005"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:223..225,aa:Lys,seq:ttt)
     gene            complement(413..763)
                     /gene="ssrA"
                     /locus_tag="VFQ79_24010"
     tmRNA           complement(413..763)
                     /gene="ssrA"
                     /locus_tag="VFQ79_24010"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(790..2934)
                     /locus_tag="VFQ79_24015"
     CDS             complement(790..2934)
                     /locus_tag="VFQ79_24015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142808.1"
                     /translation="MPSFKVIDQLGVDIDVEPNPDSGLAMYARELTRFRLPEVNWERT
                     ANLPLEDAPVNTGGAGLSFDQPVPVGSAGELTIGAGVSGRLRLFRSVDGELFASGLSG
                     DPIPIEPEEAYLAISITASVSGGASNAAGRLGFGVDAGREVTITNYRPFATGTPFADA
                     FRESVAGFVFPATVEDLKRIPGRGIVTVEGQGQLRFSGDADLLTLSNPLATLSLPAPA
                     GDVRVVAGGSVTIGASYTLSSEHQVRLYKTAAGSAQIGYYRCGSGEFSIRASARAGAG
                     VLVRNYNPLAMVLRAISADAAADEDALEHAGASGSDISSIRDAVEAAIDRKLELAASF
                     ELGALSQRDAAFLYEVDPGTLDSAGFEMVERALQGDLPPLTEAGELLPPGIRMVRSVL
                     GTLRRHQQTLKVNLLGIYNFVSISQLALSGSVLYEPATGDLVITDTATAQRVRASAVN
                     FGADTQKLRNVLAESFLITAVYRSGKIARPLPELRVRQTYFELHNATNHQTMKDNLDI
                     AWALGLISTSQQQNALEGTEDFGRSTVYAEASYGESETAALFMKQDGEARKVQEYEAA
                     GRRAIAMLIQPGDPNEYRRIPATDDEIWKRMKSAGQPGFRQIFPSCSQVQVETLIADY
                     SLIVWWSRSMADLAAALHQIRSKGLDVETARKTLTRRLKSVAAHTQPQFGDPWGLIAM
                     DQAAGQRAEVACQITSSRLAWRGERKEKAVTA"
     gene            3047..4252
                     /locus_tag="VFQ79_24020"
     CDS             3047..4252
                     /locus_tag="VFQ79_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142809.1"
                     /translation="MFACAARAEPAPGNQAEKARFFGEIAAIMAELEEISGLDARARV
                     RYDTITRPELRRYLERKIQEEVKPEEIRADELALKKFGLVPPDYDLKESTVDLLTEQA
                     AAFYDFRARKLFVLESASGALDQIALVHELAHALADQNFRLAKFIKRGESSDDGSLAH
                     VAVMEGQASWLTGEYLARKSGQSLKTSPALLERMSRSADDAASQFPVLKSAPLYIRET
                     LLFPYTAGMRFQNAVLEKFDLEGFRRVFKNPPVTTQQILHPQKYFDAVLPTKPRLPEL
                     PEARSYRSLTGGSVGELDHEILLRQYIGEDAAGLAERWRGGSFELFEHRKTKEAVLAY
                     AVEWNETSDARRYFELYRRILEAKWKTFQLTEESEAAVAGEGDGGHFRVELIGKIVTS
                     LEGLPSAIK"
     gene            4275..4832
                     /locus_tag="VFQ79_24025"
     CDS             4275..4832
                     /locus_tag="VFQ79_24025"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0142810.1"
                     /translation="MLKPFTVLGVIGSLLFPVLAADLPRPAPDFKFKMPNGQDASLSQ
                     YKGKVVALEFLLTTCPHCQQTSMLMERLQKEYGPRGFQALGIAINDPSGALVRQYIQQ
                     YRLTYPVGFSTNREAVVGFLQHPVMMSLMMPQLVFIDRNGNIQAQFAGTDDFFKNEEK
                     NVRQQITNLLNSAGKSTRSQRKKGS"
     gene            4837..5391
                     /locus_tag="VFQ79_24030"
     CDS             4837..5391
                     /locus_tag="VFQ79_24030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0142811.1"
                     /translation="MRVFLLLALAVVLVSGTALGADVPRKSPEFAILTPDGKQFLLSQ
                     YRGDVVALEFAFTTCPHCQHSARLLSQFKKEYGAKGFQPLIVAFNPMAGMLVPEFVSV
                     TGADFPVGASPRETVLQYLGVPDNLRLVVPQMVLIDRNGNIRYQSPADGSDKFFDERV
                     LRSRVEELLKERTGAARPASSKRK"
     gene            5468..6181
                     /locus_tag="VFQ79_24035"
     CDS             5468..6181
                     /locus_tag="VFQ79_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0142812.1"
                     /translation="MAEPRRVFSATGITKVYHMGDVEVHALRGVDLELYAAEFTVLLG
                     PSGSGKSTLLNILGGLDRVTSGEVTYEGRDLSRASERDLTNYRRHYVGFVFQFYNLIP
                     SLTARENVSVVTEIARDPMRPEDALELVGLGDRMDHFPSQLSGGQQQRVAIARAIAKR
                     PAVLLCDEPTGALDSETGIVVLEALMRVNREMGTTTAIITHNADIALIADRVIYLSDG
                     HIVRAEQNREKKEPHELHW"
     gene            6185..8545
                     /locus_tag="VFQ79_24040"
     CDS             6185..8545
                     /locus_tag="VFQ79_24040"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142813.1"
                     /translation="MRAINRKILRDLVGMRGQALAIALVVASGAATYIMSVSALDTLE
                     STLAAYYREYRFADGFVSLKRAPKSVAARLREIPGIRMLETRVLASANLTIENFDEPV
                     SAQIVSVPENGPLLNAIYLRSGRMPDPSRDYEVLVSDSFASAHKFRAGSHFYATINGR
                     RRRLEITGIALSPEFIYQVQPGALIPDFKTFGILWMSEDALEAAYDMEGAFNDVTFSI
                     ERSANIKEIIDRVDEILARYGGLGAYGREDQLSNKYLSSEFDQLRQMAVIFPAIFYGV
                     AAFLLNIVITRLIATQREQMGVLKAFGYTTADLVLHYLALVAMIIAVGIAGGIALGAW
                     LGQALGSLYMEYYRFPFLLFHLRPGMIFMAALISCVAAGLGTFHAVSRAASLPPAVAM
                     QPPAPPRYRRSFLESLPLARSVSQPTRMIVRNLERRPLKSVLSILGVAAACAILVVGT
                     LFSDSVDYIVRIQFQFAQHDDLNVTFNEPASAKAISSLKAMPGVRYAEPFRSVPVRLR
                     FEQRTYRTSIQGLPHGAVLRRVLDSELKPAPVHEHGLLLTDYLAGILHVRPGDTLTVE
                     ALEGERRVLRLPLVAVVKEFVGVGAYMELGALNRAMGEAPSISGAYMETDSAHDKKIY
                     AELREMPRVAGAGVRANALRNFYETMAKQALTFAFFNTLLASSIAFGVVYNSARIGFS
                     ERSRELASLRVLGFTRFEVAYILLGELAVLTVAAIPIGFFMGRGLGAFMISNIQTDLF
                     RVPVIVDPSTYAFAAIVVLASALISGAGILRKIYKLDLVEVLKVRE"
     gene            8549..9739
                     /locus_tag="VFQ79_24045"
     CDS             8549..9739
                     /locus_tag="VFQ79_24045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0142814.1"
                     /translation="MHWRRWTVIILIAAGIVAAIAYSLWPQPVPIEVAEVARGPLSVT
                     VEEEGKTRVKERYVIYAPVTGYNRRLDFDAGDPVKQGQVVALIEPSRPVPLDVRSLAE
                     GRAAVAAAEAQVQAAEQRIRAATANAEYWSAELKRVNDLVRTGDIARSRLEQTVSEQQ
                     RAAAALAEAQHAMEAAQSELRRARAAITEFTGTPQATDIVEVRAPVGGRVLKLLRESA
                     GAISVGEPLLEIGNARAIEVEIEVLSPDAVRIKPGTSVVFTRWGGDGPLEGIVQRIEP
                     SGFTKISALGVEEQRVPVIAILTSPETAWSGLGAGYRVEASFILWQGNGVLQVPTSSL
                     FRYNDGWAVFVVDGGIARRRQVELGHRTGLRTEITNGLQPGEQVIAYPDETIEDGTPV
                     EPRA"
     gene            complement(9747..10688)
                     /locus_tag="VFQ79_24050"
     CDS             complement(9747..10688)
                     /locus_tag="VFQ79_24050"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0142815.1"
                     /translation="MERNLSRRTLLASAAGVAASALPATAATGRGQLGCMTMLYRAHP
                     LDTALQGIRKAGFQYVVPARAHAGKDVFAPAMPSAARAELKAKLKDHGLTANMSLGGF
                     GPELGRPGGLEKYIAELDLCADFGIPVIVGGGPWYFQKWPNLPKSDRDWQKEVAAFYN
                     DLEKAVRHAGSVKVIITLKPHTGITARAKECMEVVKRLPSEWLKICWDAGNVSFYEGI
                     YPDPDLPDLAPHVKAVCIKDHLGGRAEGNFPLPGAGQIDHDLMFRTLFGAGFNGPMGV
                     EKVEGRDNVMKMPAAQIDERVAQAYNFLTAAIEKASS"
     gene            10863..14147
                     /locus_tag="VFQ79_24055"
     CDS             10863..14147
                     /locus_tag="VFQ79_24055"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HEU0142816.1"
                     /translation="MSARHLCVFTSCLLLSVFTALAAQSSSAVKTYEATLEIPTYEHT
                     ARELEPPLFGNSSVTGLYPFTTYRMPFKPGGPKPRKYRSIHLENEYLKLTYLPELGGR
                     IFSVYDKVRGREMFYRNDVIKPAGYNPRNSWHQSGIELTGPYDAHMLTLYGEPFWSNK
                     VIPNADGSMTLMLGELDPVYRMRVDLSATLYPGIAAVKMGVFCYNTRDGRMPQMFWLS
                     AALPGTEKTQFIYPMTRTIGHTTSEIADWPVHNGVDYSWDRNNQNMLGVFGIDIYDDF
                     QGAYHHDLDYGVFRYADRRVVQGMKMWTFGYGPNAKSYERGYTDAAGPYVEVQSGRHV
                     WDGHYEWVYPHKAESWSEWWVPVSGIGGLTTLSRDVALNLKVDAGAPARLALAATRPL
                     PGARIIVKSDAGVILDTAADLAPGKPYVKDLGPLQSGATGVVVMVTEAGGREVLHYAR
                     PDSNPGRKEYTPFTRSLEKPQKTLEQMTVEELVLAARFKLKELNSAGGVDLLNKALER
                     DPGFSRAHLEYGIHHFNHYRYREAVKHLEQVIERDPYVDEAYYYLSISQLRLGETAKA
                     ERNFYYIWPGSPFYSDREYNLARLALGKSDFDEAVTHLRKAIEVNGRHQGARALLTIV
                     SRERENRAGALEQIEAIERMDPGNRIAQAERWLLTGDERARAELARLMGGQSQEAIGM
                     SSFYRDAGRWNAAAKILKLVETNNADRWGTSPEFYYILAYCLHQSGSQQEASRYLVKA
                     RSAAGNVDRFPYRENSKPAFEWAIAQDPQDALALYLQGALDYYLERFGEGIRSWEQAV
                     HAKPDDFRSRRALGLAYAEQGKPVETAAGQLEKAVALNPDHVATLNDLSALYAKAGRF
                     DEQLAVLQRALKRSPGDDNLAEGVLTANLIKGHYDAAEQLVASHRFAPRHRSYGLRDK
                     YRLLRYGLGAAAFRQNRYPEAVKQFEAALQPPVSLGVDDFASQTSPEKEYYVGRALEA
                     QGDKDAARRAYERGIAGIEHLSGDSSNWSSENFHMVLTLDRLGRNEEATKLAEQFENY
                     ARTQLDSRRPARRVESRYLLGLAAERKGHSQEARKMFEEAVTLQPDFLPARMHLRGDL
                     INSFSK"
     gene            14233..17499
                     /locus_tag="VFQ79_24060"
     CDS             14233..17499
                     /locus_tag="VFQ79_24060"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0142817.1"
                     /translation="MNRSMAFLRLVALVLCCEALLVAQTTFGVIRGRVLDATGAAVTG
                     AAVVVTNTATGIARTFTTNESGAYEAGYLQPGTYSVAAEASGFKKFVADGIILNATAI
                     VLVDAKLEVGDITTSVTVEAGAPLISTESAVISDVKTAQQYLQAPMNVRGNWDSFLYN
                     FMSLVPGAQPTVNGFSISFAGTRYTMNNFTVDGITTNSTLYGNQVGPANPSMDFIQEV
                     KVDMSGNSAEFSAPGYVSVITKGGANEFHGSAYWYYNTAGLNARNFFQSRVGFAVLND
                     YGFTFSGPVFKNKTFFSGGLEAFNQHTGAALNLNLPSNRLRNGDFSMLRDSQGNPINI
                     TDPLTEQPFPGNVIPSNRLNQTALKIQERFFPLANFGDPESVVGNYREQFKQRQRKEQ
                     VDVRIDHQFSSSNAFFGRFSAMRAPNNALEGSLPTIGLRKQRRQTRNLVLSDTHTFRP
                     TVINEFRFGLIRGYNPYSGPVLGREIASELGLTGLPSDLPDVEALPIINISGFQGISQ
                     LDYQRGAEMIFQWQDNLSWIVGRHTIKMGGEVWHNYGENYGVSPSRAYGSISFTGNYS
                     GHAYADLLLGIPRSASRSSAGFVLLKSTNWDKFLFIQDDFKATPRLTLNFGLRYELNP
                     PYVETEDRLASFNPFTGQMVVPTEASKGALFPAFVNSNLVPIVTAEQAGLPTRTLAYT
                     DKNNFAPRVGFAYKVTSDNKTVLRGGYGIFYDTFTAALWRSLVGGPYNGSESAPPNAI
                     INGAPLWQLPAMFPAQVDQSGTASLGGIDPHMKNPYVQQWNLTIEREIFDMGLRVSYV
                     GTKTHQLVLSRNMNQVMPSTTPFSTSRRPFPQLSGATWRDNSGNAYYNGLTLAAERRL
                     KKGLQYQLSYTWAKNLTDNHDEWEGGGGLQNAYDRHAEWANHQYTRRHRFIASAIWEL
                     PFGKTDGWKKHLIEGWSWSTFAVLQTGPYFTPTFAGFDPSNTGSSSGRPDRIASGEID
                     NPAINRWFDVSAFRVPGDTDGDGRPDVAVGRFGNSGLNILRAPGTKSMNMGFFKKFHI
                     TETMGLQMEATFTNIFNHPNFAAPDSLVTSGSAGAVLAVQSLEGAGARTTRLGLRLDF
                     "
     gene            complement(17576..18721)
                     /locus_tag="VFQ79_24065"
     CDS             complement(17576..18721)
                     /locus_tag="VFQ79_24065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142818.1"
                     /translation="MRFFLWTLLLVFPLCGQRTLEYDRSVVSQVRIDLRELGYPPEDV
                     IPSGECAIRSLAVAPNGAIYGATSGKRSHLFALFPQHGYVQPLGFLKDVSLVHRALAV
                     SKSGDVYIGAGGSNGHLFKYVPKRDDARAIRIDAPLEVTDLGAPVAGEGIHSLAIDRE
                     RDVLYGLTWPSGQVFSYRIGSVEFVAHGKVAERNIPGENFEKHKNIGRAIAIHSSGFA
                     LMSGENGRIFRVKALGTGVLEPLDLHAPTVRGREPYNGVDSWAESESGILYGGTSDGY
                     LFRLDPKSWKIENLGKPLNQYRIRGLAFAKNGKLYGVGGDDDEMARLFSYDPASGVYE
                     MLGMIDVNRRPYYSWQAYVIDAVAAGLDGTLYFGQSERKSKLYLYHP"
     gene            complement(18731..19972)
                     /locus_tag="VFQ79_24070"
     CDS             complement(18731..19972)
                     /locus_tag="VFQ79_24070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142819.1"
                     /translation="MLLLAPSGAQTPQKTIRIDHRVFTGLEEMDGNWAALLAASDGKV
                     YAGLAYHGGDGHLVYYDSKTDRMHDVGDLTRLSGESNLKRGPQSKIHAKFGEGKDGRI
                     YYGTHGGIWWNYSRFATKEGYPGAHWAAYDPKTNRVEDFGLGVPNEGVNTGAYDPLYN
                     RIYGLTHPRAHFVYYDVKTGKAVDLGRINNWESICRTLGVDDQGNVYGSFGMGQIFKY
                     DPKTDEIEELPVRLPIREKGISLGRDYHKSETAWRTVVWDTKTRQFYGVEESATILFS
                     FNPRDNTIQRLGQLAIPKFADRRDVPYATLSLTLGRDRKLYYGASGREFDYGGSAGLA
                     TAHLITYDLASGKTEDLGEMLLEDGRRVLGTNAADTGPDGTIYMVGAIEVRPDANKKV
                     EAAGKIGDAYYRLALLIYRPR"
     gene            complement(19989..22100)
                     /locus_tag="VFQ79_24075"
     CDS             complement(19989..22100)
                     /locus_tag="VFQ79_24075"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEU0142820.1"
                     /translation="MIFLRLLLAGVLLSVPILAQIGNATWATPVKKGFAESAWWDQGV
                     VFVGNWEPLVFRLRHGGNLPENVEELYRKEHTEETVLKLKQAGVNMILTHFYKTGLDS
                     EREDVELAKRLGVLCHKHGMKLGTYIGGTIFAETLLRDLPEAKEWVRYDEYGNAIRYG
                     EQTYRYRPDFNHPGYVEYMKRIARIAIEEVKTDFIHFDNHALIAPPYTGNTPEINRRF
                     REFLRKKYTPELLKQRFGFADISAITVPAWHNISRPATISPITDPVMQEWVDFRCADL
                     AAYYGEVADYIRKLNPNVLVELNPHGIYGSNRAFLNGIDHARLVPHGSVFWSEEPNEA
                     QVDANGILVSKIRSMKLARSLDQTLFSYTGPQRADAELRSYRLLMAESLAFNRNSLGD
                     LGSPLSAYEFPGDLLRYVRFYLDSNRYYSGTRTVADVAILRSFPSMAYNSAGPHLHTT
                     LLEQLLIQYKVPFDIIFDQNLADLSKYRTLVLGDQESLPDRALAQIRDFVKAGGGLVA
                     TGRTSLYNDWRRIRNDYGLADVLGIHSVRGRAMPEEQRGTFGKGRAVYVRDVVAADPI
                     PGIEADAPNSPTGASGFGRAYWKLPKNSEQLVAAIRYANGAPLAVEFEHAPLTTVMEI
                     TEKKDGSGRMLHWVNYRLGTPVPSTTVSVSVPPDKTVRSVEVISPDGNASGKVKFTME
                     KNRVRFQLPALEVYNVALINY"
     gene            complement(22108..>23517)
                     /locus_tag="VFQ79_24080"
     CDS             complement(22108..>23517)
                     /locus_tag="VFQ79_24080"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEU0142821.1"
                     /translation="LRTKYTAAERQARFGFANIDPVNPPGWNRQTPPERMEIIFDPAI
                     QEWIDFRCQVMSDALGQMAKLAKSVNPEVVIEVNPHGIQGGNRAWEAGLDHSRFLKHT
                     QVFWTEEHNAPEYLDDGRLISTIRSYKLGRRYNNIVFTYIAGDPAAMAESLAFNQTIG
                     YAGNDPLPKNMLKYIAFYKQHRDYFTGAVDAGNVALLRSYPSITYHNARAQLSAILME
                     QALIQSRIPFDLIFDEHVADLSKYRVLILPDSQCLSDVQLASIRTFVQNGGGLVATGL
                     SGMYDQWRRLRMKPGLLGLVDSQRPARGYEENVQRSEEAGRHVTKEFGKGRVVHFPSI
                     PFDGDLPPMTNYFKIDRRFWKAPPNWAETAKLVRWAAREEIPVTISGPPYLVANLVAQ
                     PENRRSMIHLINYNHRNTPEISPVEITCSIPKGEAVKQVRILSPDDAPRTIEARPAPG
                     GAAFTVPLLKTYSIAVVNW"
BASE COUNT         4845 a         6808 c         6992 g         4872 t
ORIGIN      
        1 ggtgcggctg tcggtaggtc tcgaggattt ggacgatatt ctctgggatc ttgaccaggc
       61 cctcgcggca tcgcaggcgt agaattcagc aggcgtgtta gactgcaatt gatacgcggg
      121 gtgtgcccac gcccgtttcg gcgggggcgt catcgtgaca gtcgtccttc caggtcatcc
      181 gacaaaaacg ggccggtagc tcagtcggta gagcatatgc cttttaagca tagggtcgcg
      241 ggttcgagtc ccgcccggct caccaagttt cacagattca ataacttagg accaaagggc
      301 cgatggcata gaaaggtaaa tgacactatg cctcctgctt tcccacaggg cagtctcagg
      361 gcgctattca ccgccaagcg ggagtcatct ggtatgaaac cacgggaaga aatggtggag
      421 gcggcgggag tcgaacccgc gtccgagaaa gcttgccgtg aaaagcctac gtgtgtatcc
      481 gattctgagt ctcaattgcc gcctgagaac cggcaagata gcggcaatcc agttcgattg
      541 gatctcggct tacggctccg aacagaagcc ttgggcccat ctcgccatat gacgctcact
      601 caccggacat gcgagcctgc cggctggagc ggctacctaa ttacttaggc agcgtaagca
      661 aactgctgat tggcagttat ggttttccga tcgtttaacg ggtgctcgga acccgacacg
      721 ccttcgcacg ccaattcaat cccgtcgaat ccgttacgcc ccctttcaac tactattgta
      781 gcacttgccc tacgcggtca ctgctttttc tttgcgttct ccgcgccatg ccagtctcga
      841 actggtgatc tggcaagcca cttccgctct ttgcccggcc gcctggtcca tcgcgatcag
      901 cccccacggg tcgccgaatt gcggttgcgt atgggcggcg acggacttca gccgccttgt
      961 cagagtcttt cgcgctgtct ctacatccag tcccttgctg cggatctggt gcaaagcggc
     1021 cgccagatcc gccatcgagc gggaccacca tacaatcaac gaataatcag cgatcagcgt
     1081 ctccacctgc acctggctgc agctggggaa gatctgccgg aaccccggtt gtcccgccga
     1141 cttcatccgt ttccaaattt cgtcgtccgt cgcagggatg cggcggtatt cattcgggtc
     1201 tcccggctga atcagcatgg cgatagcacg gcggccggcg gcttcgtact cctgcacctt
     1261 ccgggcttca ccatcctgct tcatgaataa cgcggcggtc tccgattctc cgtaggatgc
     1321 ctccgcgtaa acagtggagc ggccaaagtc ttcggtcccc tccagtgcat tctgttgttg
     1381 ggatgtcgaa attagcccaa gcgcccaggc gatatcgagg ttatctttca tggtctggtg
     1441 gttcgtagcg ttgtgtaatt cgaaataggt ctgtctcact ctcagttccg gcaagggccg
     1501 ggcgatcttg ccggaccggt agacggccgt gatcagaaag ctttccgcca aaacattccg
     1561 tagcttctgt gtatcggcgc cgaagttcac cgcggatgcc cgtacgcgct gcgctgtggc
     1621 tgtgtcggta atcacgaggt ctccggtagc cggctcatac aaaacacttc cgctaagagc
     1681 aagctggctg atggagacga agttgtagat gccaagcaga ttcaccttca atgtctgctg
     1741 gtggcggcgc agggttccaa gaacgcttcg gaccatccgg atccctggag gcaatagctc
     1801 tccggcttcg gtgagcggcg gcaggtcgcc ctgcaacgcc cgctccacca tttcgaagcc
     1861 cgcgctatca agcgtgccgg ggtccacttc ataaaggaaa gccgcatcgc gctggctcaa
     1921 agcgcctagt tcgaaactgg ccgcgagttc gagcttgcga tcgatcgccg cttccacggc
     1981 gtcgcgaatc gagctgatgt cgctaccgga cgcccctgcg tgttccaacg cgtcttcatc
     2041 tgcggctgcg tccgcgctga ttgcgcgcag caccatcgca agaggattgt agttgcgaac
     2101 cagtaccccg gctccagctc tcgctgaagc gcgaatggaa aactcgccgc tgccacaacg
     2161 gtaatagccg atttgggccg aaccggccgc tgtcttatat aagcgcacct ggtgctcaga
     2221 ggagagtgta taggaggcgc cgattgttac cgatcctcct gcgaccacac gcacgtctcc
     2281 ggcgggagca ggcagggaga gcgtcgccag cggattcgac agcgtgagca aatcggcatc
     2341 gcccgaaaac cgaagctgcc cttgtccttc cactgtgacg atgccgcggc cgggaatccg
     2401 tttcaggtct tctacagtag ccggaaagac gaagcccgct acgctttctc gaaaggcatc
     2461 ggcaaaaggc gttccggttg cgaaggggcg gtaattggta atcgtgactt cccgtccggc
     2521 atccacgccg aaaccgaggc ggccggccgc attcgatgct cctccggaga ccgaagctgt
     2581 aatcgaaatc gccaggtagg cttcctccgg ctcgatcggg atcgggtcgc cggagagtcc
     2641 ggaggcaaac aactcgccat ccacggatcg aaagagacgc agacggccgc tgacccccgc
     2701 gccaatggtc aactcaccgg cactcccgac gggaacgggt tggtcgaagg agaggcccgc
     2761 gccgccggtg ttcacggggg catcctccag aggcaggttg gcggtccgct cccagttcac
     2821 ctcgggcaac cggaatcgcg tgagctctct cgcatacatt gcgagcccgg aatcgggatt
     2881 cggctcgacg tcgatatcga cgcccagttg atctatcact ttgaacgaag gcatacgtcc
     2941 tttcgtgtgc gcgcggtttg ccgcacccgg gtagttcacc accccggttt atactctaaa
     3001 gcaagtgggt ctaaaccgaa aacaaatctg tactatcgcg gtactggtgt tcgcatgcgc
     3061 cgcaagagct gagccggctc cgggaaacca ggccgagaag gcgcgtttct ttggcgagat
     3121 cgcggccatc atggcggagt tggaagagat cagcggtctc gatgcgcgtg ccagggtgcg
     3181 ctatgacacc atcacccggc ctgaactgag gcgctatctc gaaagaaaaa tacaggagga
     3241 ggtgaagccg gaggagattc gcgccgatga gttggcgctg aaaaaattcg gcctcgttcc
     3301 gccggattac gatctcaagg agagcacggt cgatctgttg accgaacagg cggcggcttt
     3361 ctacgatttt cgcgcgcgga agttattcgt gctcgaatcc gcatcgggtg cgctcgacca
     3421 gatcgcgctt gtacacgagt tggcccacgc cctagccgac cagaatttcc gtctcgcgaa
     3481 gttcattaaa cggggggaat cgagcgacga cggctctctt gcgcatgtgg ccgttatgga
     3541 aggacaggcc tcgtggctca ccggcgagta cctggcgcgc aagtcggggc agtcgctcaa
     3601 gacatcgccc gcgctgttgg aaaggatgag ccgttccgcg gatgatgcgg ccagccagtt
     3661 tcctgtcctg aagagcgctc cgctttacat tcgcgaaacg ctcttgttcc cctacacggc
     3721 cggcatgcgg ttccagaatg ccgtgctgga aaaattcgac ctcgaaggct ttcgccgcgt
     3781 attcaagaac cctccggtga ccacgcagca gattcttcac ccgcagaagt acttcgatgc
     3841 cgtcctgccc acgaagcccc ggctgccgga attgccggag gcgcgatcgt accgcagctt
     3901 aacgggaggt tccgtcggcg aactcgatca tgaaatcctg ctgcggcagt acatcggcga
     3961 agatgccgcc gggctggcgg agcggtggcg tggaggaagc ttcgaattat tcgagcacag
     4021 gaagacgaag gaagccgtac tcgcgtatgc cgtggaatgg aacgagacga gtgatgcccg
     4081 ccgctacttc gagctctatc gccggattct cgaggcgaag tggaagactt ttcaactcac
     4141 ggaagaatcg gaagccgcgg tggccggcga gggtgacggc ggccacttcc gtgtagagct
     4201 gattgggaaa atagtaacca gcctggaagg cctaccgtct gcgataaagt aagaacatag
     4261 aggagatttg cttagtgctc aagcctttca cagttctggg cgtcatcggc tcgctcctgt
     4321 ttcctgtcct tgccgcggat ctgccacgcc cggctcctga cttcaaattc aagatgccta
     4381 atggccagga tgcgtccttg agccagtata aagggaaggt ggtggccctc gagtttttgt
     4441 tgaccacgtg cccgcactgc cagcagacat cgatgctgat ggaaagactc cagaaggagt
     4501 atgggccgcg aggctttcag gcgctgggta ttgctatcaa cgacccttcc ggcgcgctcg
     4561 tccgccaata tatccagcag taccggctga cgtatccggt gggtttcagc acgaaccggg
     4621 aagcggttgt cggcttctta caacacccgg taatgatgag tctcatgatg ccccagctcg
     4681 ttttcatcga ccggaacggc aacatccagg cgcagttcgc cggcacggac gatttcttca
     4741 agaacgaaga aaagaacgtt cgccaacaga tcacgaatct tctgaatagc gcaggaaaga
     4801 gcacgcgatc tcaacgaaag aagggctcct gaggcgatgc gggtatttct gttgttggca
     4861 ttagcggtgg tattagtgag cggaacagcc cttggtgcgg atgttcccag gaaatctccg
     4921 gaattcgcaa tactgacacc cgacgggaaa caatttttgc tgagccagta ccgtggagat
     4981 gtggtcgccc tcgagtttgc gttcaccacc tgtccccatt gccagcattc ggctcgtttg
     5041 ctgtctcagt tcaagaaaga gtatggagcg aaggggtttc aaccactgat cgtggctttc
     5101 aatccgatgg caggcatgct ggtacccgaa tttgtgagtg tgaccggagc ggattttccg
     5161 gtgggggcca gtccgcgcga gactgtattg caatacctgg gggttcccga taacctgcgc
     5221 ctggtggtgc cgcaaatggt tcttattgac cggaacggca acatccgcta tcaatcaccg
     5281 gccgatggca gcgataaatt cttcgacgag cgggtattgc gaagccgcgt cgaggagttg
     5341 ttgaaggaac gcacgggcgc cgcacgtccg gccagcagca agcgaaaata gacacgcggg
     5401 ttctgctgca ggccagtaat caaagatcaa cttttatgcg gtaagcgagg catgtccggg
     5461 tccactcatg gcagaaccgc gccgcgtttt ttcggcaaca ggtattacga aggtctacca
     5521 catgggcgat gtggaggtgc acgcgttgcg cggggtggat ctggaattat atgccgccga
     5581 gtttaccgtg ctcctcgggc cgtcgggcag cggcaagtcc acgctgctga atatactggg
     5641 cggtctcgat cgcgttacat ccggtgaggt tacttacgag ggacgcgacc tgtcacgggc
     5701 atccgagcgt gacctgacga actaccggcg ccattacgtc ggttttgtct tccagtttta
     5761 taacctgatt cccagtctta ctgcgcggga aaatgtttcg gttgtgaccg agattgcgcg
     5821 cgatcccatg cggcccgagg atgctctcga actggtgggg ttgggggatc gcatggacca
     5881 ttttccgtcg cagttgtccg gcgggcagca gcagcgcgtc gccatcgcca gggcgatcgc
     5941 gaaacgtccc gcggtgttgt tgtgcgacga gcccaccggc gcgctcgatt ccgaaaccgg
     6001 aattgttgtg ttggaggctc tgatgcgcgt gaatcgcgaa atgggcacca ccaccgccat
     6061 catcacgcac aatgccgaca ttgccttaat tgccgaccgg gtgatttatc tgagcgacgg
     6121 tcacattgtg cgcgcagaac aaaaccgtga gaagaaagag ccgcacgagc tgcactggtg
     6181 acgcatgagg gccattaacc ggaagatcct ccgcgatctc gtgggaatgc gcggccaggc
     6241 gctcgccatc gcgctggtgg tggcaagcgg cgccgcgacc tacatcatgt cggtgagcgc
     6301 gctcgacacg ctcgaatcca ctctcgccgc ctactatcgt gagtaccgct tcgcggatgg
     6361 attcgtgtcg ctaaaacggg cgcccaagag cgtggcggcc cgccttcggg aaattcccgg
     6421 catccggatg ctggagaccc gggtgctcgc atcggctaat ctcacgatcg aaaatttcga
     6481 cgaacctgtc agtgcgcaaa tcgtctccgt gccggaaaac ggcccgttgt tgaatgccat
     6541 ctatcttcga agcggcagaa tgccggatcc gtcccgcgac tacgaagtgc tggtgagcga
     6601 cagcttcgca agcgcgcata aatttcgtgc gggcagtcat ttctacgcca ccatcaacgg
     6661 gcggcggcgc agattggaga ttaccggcat cgcgctctcc ccggagttca tataccaggt
     6721 gcagccgggc gctctgattc cggacttcaa aaccttcggc atcctgtgga tgtccgagga
     6781 tgccctcgaa gccgcctacg atatggaagg cgctttcaac gacgtcacat tctctatcga
     6841 gcgaagcgca aacatcaaag agatcatcga ccgcgtggac gagatcctcg cccgctacgg
     6901 cggccttggc gcatatggac gcgaagacca gttgtcgaac aaatatctat ccagtgagtt
     6961 cgaccagctc cggcagatgg ccgtcatttt cccggcaatc ttttacggcg tcgcggcgtt
     7021 cctgttgaat atcgtcatca cgcggctgat cgccacccaa cgcgaacaga tgggcgtgtt
     7081 gaaggctttc ggatacacga cagccgatct cgtactgcat tacctggcgc tggtggcaat
     7141 gattattgcg gtgggcatcg ccggaggaat cgccttggga gcgtggctgg ggcaagctct
     7201 gggcagcctg tatatggaat attaccgctt tccatttcta ctgttccatt tgcgcccggg
     7261 catgattttc atggcggccc tgatcagttg cgtggccgct ggattgggaa cattccatgc
     7321 ggtctccagg gcggcgtcgc tgccgccggc ggtagcaatg cagcctccgg ctccgccgcg
     7381 ctaccggcgc agtttccttg agtcgctacc gctggcgcgc tcggtctcac agccaacacg
     7441 tatgatcgtg cgaaacctgg agcgccggcc tctcaagtcc gtgctctcca tcctcggtgt
     7501 ggcggcagct tgcgccatcc tggtcgtcgg cacgctgttc agcgattccg tagattatat
     7561 cgtccgcatt cagtttcagt ttgcgcagca tgacgatctc aacgttactt tcaatgagcc
     7621 ggcgtccgcc aaggcaattt cttccctcaa agcgatgccc ggcgtccgct acgccgagcc
     7681 attccgttct gtcccggtgc gcctgcggtt tgaacagcga acctatcgca cgtcgattca
     7741 gggattgcca cacggtgcgg ttttgcgcag ggtgctggac agcgagttga agccggctcc
     7801 tgtacatgaa cacggcctgc tcctcacgga ctatctcgcc ggcattctgc atgtgaggcc
     7861 cggcgacacg ctgacggtgg aagctctcga aggcgaacgc cgggtgctcc ggcttcctct
     7921 tgtggccgtg gtgaaggaat tcgtgggcgt cggagcgtac atggagctgg gcgcattgaa
     7981 ccgcgcgatg ggtgaggcgc cttccatctc gggagcgtac atggaaaccg attcggcgca
     8041 cgacaagaag atctacgccg aattgcgcga gatgccacgc gtggccggcg cgggcgtgcg
     8101 ggcgaacgcg ctgcggaact tctatgaaac catggcaaag caggcgctca cgtttgcgtt
     8161 cttcaatacg ctgctggcga gcagcatcgc gtttggcgtc gtatataaca gcgcgcgcat
     8221 cgggttttcg gagcgcagcc gcgaattggc aagcctgcgc gtgcttggtt tcacgcgttt
     8281 cgaggtagcc tacatcctgc taggtgagct tgcggtattg acggttgccg cgattccaat
     8341 cggatttttt atggggagag gtcttggagc attcatgatc agcaatatac agaccgatct
     8401 gttccgcgtt ccggtgatcg tcgatcccag cacgtatgcg ttcgccgcca tcgtggtgtt
     8461 ggcatcggcg ttgatttcgg gcgcgggcat cttgcggaag atctataagc tcgacctggt
     8521 ggaagtgctg aaggtgcggg agtagcgcgt gcattggcga cgatggaccg tcatcatatt
     8581 gatcgccgcc ggcatcgttg cggcgattgc atattcgctg tggccacagc cggttcctat
     8641 tgaagtggcg gaagtggctc gcggtccgct cagcgtgacc gtggaagagg aaggcaaaac
     8701 gcgagtcaag gagcgttacg ttatctacgc gcccgtgacc ggatacaaca ggcggcttga
     8761 tttcgacgcc ggcgatcctg tgaagcaggg gcaggtggtc gcactcattg aaccttcgcg
     8821 gccggttccg ctcgatgtgc ggagcctggc ggaagggcgg gcggccgttg ccgctgccga
     8881 ggcccaggtg caggcggccg agcaacggat ccgcgcggcc acggcgaatg cagagtattg
     8941 gtccgcggaa ctaaagcggg tgaatgacct ggttcggaca ggcgatatcg cccgttcgcg
     9001 gctggaacag acggtttccg aacagcagcg cgcagcggcg gcgttggccg aagcacagca
     9061 tgcgatggag gctgcacaat cggagcttcg gcgagcccgc gcggcgatca cggagttcac
     9121 cggcacaccc caagccaccg acatcgtgga agtgcgcgca cccgtcggcg ggcgtgtgct
     9181 gaagttgctt cgtgagagcg cgggagcgat ctcggtggga gaaccgctac tggaaattgg
     9241 caacgccaga gcgatcgagg tggaaatcga ggtgctttcc cccgatgcgg tccggatcaa
     9301 gccgggaacg tccgtcgtat tcacgcgatg gggcggggat ggcccgctcg agggtattgt
     9361 gcagcggatc gaaccttcag gcttcacaaa gatatccgca cttggagtag aggaacaacg
     9421 tgttccggtc atcgccatcc tcacctctcc ggagacggcc tggagtggac tgggcgcggg
     9481 ctaccgtgtg gaggcgagct tcattctctg gcagggcaac ggcgttttgc aagtgccaac
     9541 cagttcgctg ttccggtaca acgacgggtg ggcggtgttt gttgtcgatg gaggcatcgc
     9601 gcgccggcgg caggtggaac tcggtcaccg taccgggctg agaaccgaaa tcaccaatgg
     9661 gctccagccc ggagaacagg tgatcgccta cccggatgag acgatcgaag acggaacccc
     9721 ggtggagccg cgagcatagc gctgcatcag gacgacgctt tttcgatggc ggcggtcagg
     9781 aagttatacg cctgcgccac gcgctcgtcg atctgggcgg cgggcatctt catgacgttg
     9841 tcgcgccctt ccactttctc gacgcccata gggccgttga agcccgcacc gaacaacgta
     9901 cggaacatca ggtcgtgatc gatctgtccc gcgccgggta gggggaaatt cccttcagcc
     9961 cggccgccca gatggtcttt gatgcaaacc gccttgacgt gcggtgcaag atccggaaga
    10021 tccggatcgg ggtagatgcc ttcgtagaac gatacattcc ccgcatccca acagatcttg
    10081 agccattcgg aaggcagtct tttgacaacc tccatgcatt ccttcgcgcg cgcggtaata
    10141 ccggtgtggg gcttcagcgt aatgattact ttcaccgaac cggcgtggcg caccgctttt
    10201 tccagatcgt tgtagaaagc ggcgacctct ttctgccagt cccggtcgct cttcgggagg
    10261 ttcggccatt tttggaagta ccaaggtccg ccgccgacga tcaccggtat gccgaaatcg
    10321 gcgcagaggt cgagctccgc gatatatttc tcgagcccgc cgggccggcc cagttcgggc
    10381 ccgaatccgc ccaggctcat gttcgctgtc aggccgtgat ctttgagctt ggctttcagc
    10441 tccgcacgcg cggcggaagg catcgcaggt gcaaacacat ccttgccggc atgcgcccgt
    10501 gccgggacca cgtattggaa accggccttc cggattccct ggagcgccgt atccaacgga
    10561 tgggcgcggt agagcatcgt catgcagccg agttgtcccc gcccggtggc ggcagttgcc
    10621 ggcagtgcgc tcgccgcaac acccgcggcg ctggccagca gtgttcttct cgataagttc
    10681 cgttccattg ccattcagcc cggattataa cgtgagcaca gtgcggcacg aaaatgctgt
    10741 ttttgccggt caaccactcc ctggcggtcg tggctcagaa accgagccgc gcgcgcaagc
    10801 aagcggtgcg gcgcgtgtaa gccggagaac gatacactta cctgtagatt caaaaaatca
    10861 ttatgtccgc acgtcatctt tgtgttttca cctcatgtct gctcctttcg gtttttacgg
    10921 cattggcggc ccaatcgtcc tctgctgtaa agacctacga agccacgctg gagatcccaa
    10981 cctatgaaca cactgccagg gagctcgagc cgcccctctt cgggaattcc tccgtaacgg
    11041 gcctttatcc tttcacgacg tacagaatgc cgttcaaacc aggcggcccc aaaccaagaa
    11101 aataccggtc gattcatctc gaaaacgagt acttgaagct cacgtatctg ccggaactcg
    11161 gcggtcgcat cttctcggtc tatgacaaag ttcgagggcg ggagatgttc taccgcaatg
    11221 acgtcatcaa gcccgcaggt tacaacccgc ggaatagctg gcatcagtcc ggtatcgagc
    11281 tcacgggtcc ttatgacgcg cacatgctca cactgtatgg cgagccgttt tggtcgaaca
    11341 aagtgatacc gaatgccgac ggcagcatga cattgatgct gggcgagttg gatcccgtct
    11401 atcgcatgag agttgatctc tcggcgacgc tctatccggg aatcgccgcg gtgaagatgg
    11461 gcgtcttctg ctacaacaca cgggacgggc gcatgccgca gatgttctgg ttgagcgccg
    11521 ctctgcccgg tacagagaag acgcagttta tttatccgat gacacgcacc atcggccaca
    11581 cgacgtccga aatcgcggac tggcccgtgc acaacggcgt cgattacagt tgggaccgca
    11641 acaatcagaa catgctcggc gtgttcggca tcgacatcta cgacgatttc cagggcgcct
    11701 accatcatga cctcgactac ggcgtgtttc gctacgcaga ccggcgggtt gtgcagggca
    11761 tgaagatgtg gacgttcgga tatggaccga atgcaaagtc ctacgagagg ggctacacag
    11821 atgccgccgg gccgtacgtt gaggtgcaaa gtggacggca tgtgtgggac ggacattatg
    11881 aatgggtcta tccccacaag gcggaaagct ggagcgaatg gtgggttccc gtttccggta
    11941 tcggaggatt gaccaccctc tcccgcgacg tcgctctgaa cctgaaagtg gatgccgggg
    12001 cgcctgcacg attggcgctg gccgctactc gccctcttcc gggcgcgcgc atcatcgtga
    12061 agtctgacgc gggagtgatt cttgacaccg cggcggatct cgcaccaggc aagccctatg
    12121 taaaggacct gggaccgcta caatccggtg caaccggcgt ggtcgttatg gtgacggaag
    12181 ccggcggccg cgaagtactg cactacgccc gtcccgattc gaatcccgga cgcaaggagt
    12241 acacaccatt cacccgctcg ctcgaaaagc cgcaaaaaac gctggagcag atgacggtgg
    12301 aggaactggt gctggctgcg cgattcaagt tgaaggaatt gaattccgcg gggggcgtcg
    12361 atctgttgaa caaggcgctc gaacgggatc cggggttttc ccgcgcgcac ctggaatatg
    12421 ggattcacca cttcaaccac taccgttacc gcgaggcggt gaagcatctt gaacaggtga
    12481 ttgagcgcga cccatacgtg gacgaagcct actattacct ctccatcagc cagcttcgtc
    12541 tgggtgagac agccaaggcg gagcggaact tctactacat ctggccgggc agcccctttt
    12601 attcggatcg ggaatacaac ctcgcgcggc tcgcgctcgg taagagtgat ttcgacgaag
    12661 ccgttacgca tttgcggaag gcgatagagg tgaacggccg ccatcagggc gcgcgggcac
    12721 tgctgactat cgtaagccgc gagcgggaaa accgggcggg cgcactggaa cagatcgaag
    12781 ccattgagcg catggatccc ggcaaccgca tcgcgcaggc cgagcgatgg ctgctaactg
    12841 gtgacgagcg tgcgcgcgcg gaactggcgc gcctgatggg cggacaaagc caggaagcga
    12901 tcggaatgtc ctccttctat cgcgatgcag gccgctggaa cgctgccgcg aagattttga
    12961 agctggtgga aaccaacaac gccgaccgtt ggggtacatc gcccgagttc tactacatcc
    13021 tcgcctattg tctccaccag agcgggagcc aacaggaggc atcgcgctac cttgtcaagg
    13081 cccgttcggc tgccgggaac gtagaccgtt tcccctatcg cgaaaacagc aaaccagctt
    13141 ttgaatgggc cattgcgcag gacccgcagg atgcgctggc gctctacctt cagggcgcgc
    13201 tcgactatta tctcgagcgt tttggcgaag ggattcggag ttgggagcag gcggtccatg
    13261 cgaagccgga cgatttccgt tcgcggcgcg cgctgggcct ggcctatgcg gagcagggca
    13321 agccggtgga aacagcggcc ggacaattgg aaaaggcggt ggccctcaac cccgatcatg
    13381 ttgcgacgtt gaacgatttg agtgcactgt atgcgaaagc cgggcgtttc gacgagcaac
    13441 tcgcggttct acagcgggca ctcaaacgct cgccgggcga cgacaatctg gcggagggtg
    13501 tactcacggc gaacctcatc aaaggacact atgacgcggc ggaacaactg gttgcgtcgc
    13561 accgtttcgc tccgcgtcac cgcagctacg gattacgcga caagtaccgt ttgttgcggt
    13621 acggactcgg cgccgcggcg ttcaggcaaa atcgatatcc agaggctgtg aagcaattcg
    13681 aagccgcgct ccaaccgcca gtgtcactgg gagtggacga cttcgcatcg cagacatcgc
    13741 ccgaaaaaga gtattacgtg ggacgagccc tggaggcgca aggtgataag gatgcggcgc
    13801 gccgtgctta cgagcgcggc atcgcgggaa tcgagcacct ctcgggggat tccagcaatt
    13861 ggagcagcga gaacttccac atggtgctta cgctggatcg gctcgggcgc aatgaggaag
    13921 ctacaaagct cgctgagcag ttcgagaact atgcgcgcac tcagttggat tcacgccgtc
    13981 cggccaggcg cgtcgagtcc cgatacttgc tgggacttgc cgctgaacgc aaaggccatt
    14041 cacaggaagc ccggaaaatg ttcgaagaag ccgttacact tcagcccgac ttcctgccgg
    14101 cgcgaatgca cctgcgggga gatctaataa actccttctc aaaataagaa tgagtgggca
    14161 gtcagaatta agtgttattt agtcttcttg accactgctg ccgcaacaca taaactgggc
    14221 gacggagggt caatgaacag gtctatggcg tttctacgtc tagtcgcgct ggtattgtgc
    14281 tgtgaggctt tactggttgc gcaaaccact tttggcgtaa ttcgcggccg tgtgctcgat
    14341 gccactggcg ctgcggttac cggagcagcc gtcgtggtca caaacacggc aaccggaatc
    14401 gcaagaacgt ttacaaccaa tgaaagcgga gcctacgaag cgggttacct gcaaccaggt
    14461 acctactcgg ttgccgcgga agcaagcggt tttaaaaaat tcgtggcgga cgggattatt
    14521 ttgaacgcca cggccattgt gctggtggat gccaaactcg aagttggcga catcacaacc
    14581 tcggtcacgg tggaagccgg agcgccgctg atttcaacgg aatcggcggt catttcagac
    14641 gtaaaaacag cgcagcaata tttgcaggcg ccaatgaatg tccgcggaaa ttgggacagc
    14701 ttcctttata atttcatgtc gcttgtgcct ggcgcgcagc ctacggtcaa cggattctcc
    14761 atttcctttg cggggacgcg ctatacgatg aacaacttca cggtcgatgg catcacgacg
    14821 aactcgacac tgtatgggaa tcaggttggg ccggccaacc cgtcgatgga cttcatccag
    14881 gaagtgaagg ttgacatgtc cggcaattcg gccgagttct ccgcgcccgg atacgtcagc
    14941 gtaatcacga agggcggtgc gaatgagttt cacggcagcg cctactggta ctacaacacc
    15001 gccggattga acgcgcggaa ctttttccaa agccgtgtag gcttcgccgt gttgaatgat
    15061 tacgggttca ccttctccgg cccggtgttc aagaacaaga ccttcttttc gggaggattg
    15121 gaggcattca atcagcacac cggtgccgcg ctgaacttga atctgccatc gaaccgcttg
    15181 cgcaacggtg acttttcgat gcttcgcgac tcccagggaa atccgatcaa catcacggat
    15241 cccctcacgg aacagccatt ccccggcaac gtcattccat cgaatcgcct gaaccagacc
    15301 gcgttaaaga ttcaagagcg ctttttcccg ctcgccaatt tcggcgatcc ggagtccgtt
    15361 gtgggcaact atcgcgagca attcaaacag aggcagagga aggaacaggt agatgtccgc
    15421 atcgatcatc agttctcgtc aagcaacgcg tttttcggcc gcttcagcgc gatgcgcgct
    15481 ccgaacaatg cgctcgaagg cagtctgccg accatcggct tgcgcaagca gcggcgccag
    15541 acccgcaatc tggtgttgtc ggacacgcac accttccgtc ccacggtgat caacgagttt
    15601 cgctttggcc tgatccgcgg atacaaccct tatagcgggc cggtgctcgg gcgcgaaatc
    15661 gcctccgagc ttggactaac cgggcttccg tccgatttac cggatgtgga ggctttgccg
    15721 atcatcaaca tttcaggatt tcagggcatc agccaattgg attaccaacg cggcgcggag
    15781 atgatattcc agtggcagga taacctgtcc tggattgtgg gccgccacac cattaagatg
    15841 ggcggcgagg tttggcacaa ctacggcgag aactacgggg tttctccgtc gcgcgcgtac
    15901 ggaagcatta gtttcaccgg caactacagc gggcacgcgt acgccgatct tctgctcgga
    15961 attccaagat ccgcttcccg gtcatccgcc ggttttgtat tgctgaagag cacaaactgg
    16021 gacaagtttc tcttcattca ggatgatttc aaggcgactc cgcggctgac gctgaatttc
    16081 ggactgcggt atgaactgaa cccgccctac gttgaaaccg aagaccggct ggcgagcttc
    16141 aatccgttta ccggccagat ggtcgttcca acggaagcgt cgaagggcgc actgttcccg
    16201 gccttcgtga acagcaacct ggttcctatc gtgacagcgg aacaggccgg gctgccaacc
    16261 cgcacgctgg cttacacgga taagaacaac tttgcgccgc gcgtgggatt cgcgtacaaa
    16321 gtcacatcgg acaataagac ggttctgcgc ggcgggtacg gcatctttta cgacactttc
    16381 actgccgcgc tgtggcggtc gctggtgggt ggtccgtata acggttcgga gagcgcaccg
    16441 ccgaacgcca tcattaatgg cgctccgcta tggcagctcc cggcaatgtt cccggcacaa
    16501 gtggaccagt cgggaacagc gagccttggc gggatcgacc cgcatatgaa gaacccttac
    16561 gtccagcagt ggaacctgac cattgaaagg gagatcttcg atatgggact gcgggtgtcc
    16621 tatgtcggca ccaagacgca ccaactggtg ttgtcgcgca acatgaacca ggtcatgccg
    16681 agcacgactc cgttcagcac gtcgcgccgg ccctttccgc aactcagcgg cgcgacctgg
    16741 cgcgacaact ccgggaacgc ttattacaat ggcttgacgc tggccgccga gaggcgcctc
    16801 aagaagggtt tgcaatacca gctcagctat acctgggcga agaacctcac cgacaaccat
    16861 gacgaatggg aaggcggcgg aggattgcag aacgcttacg accggcacgc ggaatgggct
    16921 aatcaccagt acacccgccg gcatcggttt attgcatccg ctatctggga gttgcccttc
    16981 ggtaagaccg acggatggaa aaagcatctg atagaaggct ggtcgtggtc gacgtttgca
    17041 gtactacaaa ccgggccgta tttcacaccc acgtttgccg gcttcgatcc gtccaacacc
    17101 ggctcgagca gcgggcgccc ggaccgcatc gcgagcgggg aaatcgacaa tccggcgatc
    17161 aaccggtggt tcgatgtctc ggcttttcgc gtgccaggtg atacggatgg agacggccgt
    17221 cctgacgtcg cggtcggacg gtttgggaac tccggcttga atatcttaag ggcgccgggt
    17281 acgaaatcca tgaatatggg cttttttaag aagttccata tcacggagac catgggcctg
    17341 caaatggaag cgacgttcac aaacatcttc aaccatccga acttcgccgc gccggactcg
    17401 ttggttacca gcgggtcggc gggcgccgtt ctggcggtac aaagcctgga aggtgcgggc
    17461 gcccgcacaa ctcgcctggg cttgaggctg gacttttagg ccagcggcaa agaccgcgcg
    17521 gctccgaaac gcaatgattt tacggagccg cgcgcgtcag caagcggcgg tgccgttacg
    17581 gatgatacaa atagagcttc gatttccgct cgctttgtcc gaagtagaga gtgccatcga
    17641 gaccggcggc gacggcgtcg atcacgtagg cttgccatga ataatacggc ctccggttga
    17701 cgtcaatcat gccgagcatt tcgtagacgc cgctcgcggg gtcataagaa aacaggcgtg
    17761 ccatctcgtc gtcgtcgccg cccacgccat acagctttcc gttcttcgcg aacgcgaggc
    17821 cgcgaatccg gtactggttg agcggcttgc cgagattttc aatcttccat gatttcggat
    17881 cgagacggaa gagatagccg tccgacgtgc cgccatagag gattccggat tccgactcgg
    17941 cccaggagtc gaccccgttg tacggttcgc ggccgcggac ggtgggcgcg tggaggtcca
    18001 gcggttcgag cacgcccgta cccaacgcct tcacacgaaa aattctgccg ttttcacccg
    18061 acatcagggc aaagccgctc gaatgaatcg ctatcgccct accgatgttc ttgtgctttt
    18121 cgaaattctc gcccgggatg ttccgctcgg ctaccttgcc gtgcgccaca aattccacgc
    18181 tgcctatcct gtagctgaag acctggccac tgggccatgt cagcccatag aggacatccc
    18241 gctcgcgatc gatcgcgaga ctgtggattc cttcgccggc aacgggtgcg cccaaatcgg
    18301 tgacttcgag cggcgcgtca atccggatcg ccctcgcgtc gtctcttttc ggaacgtact
    18361 tgaacaggtg gccgttcgac ccgcccgcac ctatgtaaac gtcgcccgat ttcgacacgg
    18421 ccagggcgcg gtgcacaagc gaaacatctt tcaggaatcc cagaggctgc acgtaaccgt
    18481 gctgggggaa cagcgcgaac aagtggctgc gctttccgct cgtggcgccg tagatcgcgc
    18541 cattcggcgc cacggcgagt gaacggatgg cgcattcgcc ggatggaatc acgtcttcag
    18601 gaggatagcc gagttcacgc agatcgattc gaacctggct gacaacggag cggtcgtact
    18661 cgagtgtgcg ttgcccgcac agcggaaaaa ccagcagaag agtccataaa aagaatcgca
    18721 ttccgatcac ctaacgaggc cggtagatca atagagccaa ccggtagtag gcatctccga
    18781 tctttcccgc cgcttccact tttttatttg catcggggcg aacctcaatc gctcccacca
    18841 tgtagatggt cccgtcggga cccgtatccg cggcattggt gccaagcacc cgccgcccgt
    18901 cttcgagcag catttcgccc aaatcctccg tctttcccga ggcgagatcg taggtgatga
    18961 ggtgggcggt agcaaggccg gcgctgccgc cgtaatcgaa ttcacggccg gacgcgccat
    19021 agtagagctt tcgatcgcgg ccgagggtga gggagagcgt cgcatacggt acgtcgcggc
    19081 gatccgcaaa cttcggaata gcaagctgtc ccaaccgctg aatggtattg tcccgggggt
    19141 tgaaagagaa aagaatggtc gcactctcct ccaccccata gaactggcgc gtctttgtat
    19201 cccaaacaac cgtacgccag gcggtttcgg acttgtggta gtcgcgcccc agggagattc
    19261 ctttttcgcg gatcggcaga cggaccggaa gctcctcgat ttcatcggtc ttgggatcgt
    19321 acttgaaaat ctggcccata ccgaagctgc cgtacacgtt cccctgatcg tcaacgccga
    19381 gcgtgcggca aatggattcc cagttgttga tgcggcccag gtcgacggcc ttgccggtct
    19441 tcacgtcgta ataaacgaag tgtgcgcggg ggtgtgtcaa tccgtagatg cggttgtata
    19501 gcggatcgta tgcgccggtg ttcacgcctt cgttcggcac gccaagtccg aaatcctcca
    19561 cgcggttcgt cttcgggtcg tatgccgccc agtgcgcacc ggggtagcct tcctttgttg
    19621 cgaagcgcga atagttccac cagattcccc cgtgcgtacc gtagtagata cggccgtctt
    19681 taccttcccc gaacttcgcg tgaatcttcg attgcggccc gcgcttcaga ttcgattctc
    19741 cactcaaacg cgtaagatcg cctacgtcgt gcatgcggtc tgtcttcgaa tcgtagtaga
    19801 caaggtggcc atcgccgcca tggtaggcca atccggcata cactttgccg tcgctcgccg
    19861 ccagcagagc cgcccagttt ccatccatct cttccagtcc ggtgaacacg cggtgatcga
    19921 tacgaatggt tttctgtggt gtttgcgcgc ctgacggtgc gagtaaaagc accggcagga
    19981 tagccaacct aatagttgat aagagcgaca ttgtatacct cgagggcagg gagctgaaaa
    20041 cgcaccctgt ttttctccat cgtgaatttc actttcccgg acgcgttgcc gtcgggcgag
    20101 atcacctcta cagaccggac ggttttatca gggggtacgc tcacgctgac ggttgtggaa
    20161 ggaaccggag ttccgaggcg gtagtttacc caatgaagca tgcgtccgct gccgtctttt
    20221 ttttccgtaa tctccatgac tgtcgtgagc ggagcatgtt cgaattccac cgcaagtggc
    20281 gctccgttgg cgtaccggat tgccgccacc aactgctccg aattcttcgg caacttccaa
    20341 tatgcgcggc cgaaaccgga agctccggtg ggcgagttcg gcgcatctgc ttcgatgccc
    20401 gggatgggat cggccgccac aacatcgcgc acgtagacgg cgcgcccttt tccgaacgtg
    20461 ccgcgctgtt cttccggcat cgcacggccg cgaacggaat gaatcccgag cacatccgcc
    20521 aggccgtaat cgtttcgaat gcgccgccag tcgttataca aagacgtgcg cccggtggcg
    20581 acaagaccgc cccccgcctt aacgaaatcg cggatctgag cgagagcgcg gtccgggagc
    20641 gactcctggt cccccagcac cagggtccgg tatttggata aatccgcaag gttttgatcg
    20701 aagatgatgt cgaacggcac cttgtactga atcagcaact gttcaagcaa tgtcgtgtgg
    20761 agatgcggcc cggcgctgtt ataagccatc gaaggaaacg agcgcagaat cgccacgtcc
    20821 gcgacggtcc tggtgccgct gtaatagcgg ttgctatcca gatagaagcg gacataacgg
    20881 agcagatcgc cgggaaactc gtaagcgctc aacggagagc cgagatcgcc gagcgagttg
    20941 cgattgaacg ccaacgactc ggccatcaac aggcgatagg accggagttc ggcatcggcg
    21001 cgttgcggcc ccgtgtagct gaaaagcgtc tggtcaaggc tgcgcgccag tttcatcgac
    21061 cggatcttgg aaaccagaat gccattggca tccacctggg cttcgttcgg ctcttcgctc
    21121 caaaaaacgg agccgtgcgg aaccagtcgc gcatgatcga tcccgttcaa aaacgcgcga
    21181 ttgctgccgt agatcccgtg aggattcaat tcgacgagca cgttggggtt cagtttccgg
    21241 atgtagtccg ccacttcgcc gtaatatgcc gccaggtccg cgcaccggaa gtccacccat
    21301 tcctgcatca ccggatcggt gatgggcgaa atcgtggccg ggcgcgaaat attatgccac
    21361 gccggaaccg taatagcgct gatatcggcg aatccaaagc gctgtttcaa cagttcggga
    21421 gtgtatttct tgcgcagaaa ctcacgaaag cggcgattga tctccggagt attgccggtg
    21481 tacggcggag cgatcagcgc gtgattgtcg aagtgaatga agtctgtctt tacttcttct
    21541 atggcgatgc gggcgatgcg cttcatgtat tcgacatagc cggggtgatt gaagtcaggc
    21601 cggtagcgat aagtctgctc cccgtagcgg atcgcgtttc cgtactcatc gtagcggacc
    21661 cactctttcg cttcgggcag gtcgcgcaag agtgtttcgg cgaaaatagt tccgccgata
    21721 taagtgccca gcttcatgcc gtgcttatgg cagagcacgc caagccgctt tgccagttcc
    21781 acatcttcac gctcggaatc gagaccggtc ttatagaagt gggtgagaat catgttcaca
    21841 ccggcttgct tgagcttaag cacggtctct tcggtgtgct cttttctgta gagctcctcc
    21901 acgttttcgg gcaggttgcc gccgtgacgc agccggaaga cgagaggctc ccagtttccg
    21961 acgaacacga ctccctggtc ccaccaggcc gattcggcaa aacctttttt gaccggagtg
    22021 gcccacgtcg catttccgat ctgcgcgaga atcggaacgc tcagcaaaac gcccgccaac
    22081 agcaacctga gaaagatcat gggagtttta ccagttcact actgcaatgg agtacgtctt
    22141 cagcagcggc accgtgaatg ctgcgccgcc cggagccggg cgtgcctcga tggtccgcgg
    22201 cgcatcgtcc ggcgatagaa ttcgaacctg tttcaccgct tcccccttgg gaatgctgca
    22261 agtgatttcg acaggcgaaa tctctggtgt gtttctgtgg ttgtagttga tcaaatggat
    22321 catcgagcgt ctgttttccg gctgcgccac aaggttggcc accagatagg gcggcccgct
    22381 aattgttacc gggatctctt cacgcgcggc ccatcgaacg agctttgccg tctccgccca
    22441 attgggggga gctttccaga atcgcctgtc aatcttgaaa taattcgtca tcggcggaag
    22501 gtcaccgtcg aagggaatag aaggaaagtg gaccacacgt cctttaccga actcctttgt
    22561 cacgtgccgg cccgcttcct cgctccgctg cacattttct tcgtagcccc gcgcgggtct
    22621 ttgcgagtcc acgagcccga gaaggcccgg cttcatgcgc aggcgccgcc actggtcgta
    22681 catccctgac agacccgtgg caacgagccc gccgccattt tgtacgaagg ttcggatgga
    22741 ggcaagctgc acgtcggaca gacattggga atccggcaga atcagcacgc gatatttcga
    22801 gagatcggcg acgtgttcgt cgaagatcag atcaaacggt atacgcgact ggatcagcgc
    22861 ctgctccatc aaaatagcgc tcaattgcgc gcgggcattg tggtaggtga tggagggata
    22921 ggaccgcaac aacgccacgt ttccagcatc caccgccccg gtgaaataat cgcgatgctg
    22981 tttatagaac gcgatgtact tcagcatgtt tttcggaagg gggtcattgc ccgcgtagcc
    23041 aatcgtctgg ttgaaagcaa gagactcggc catggccgcg ggatcgccgg cgatataggt
    23101 gaagacgatg ttgttgtaac gccgcccgag tttgtagctt ctgatcgtcg agatcagcct
    23161 gccatcatcc agatattccg gggcgttgtg ttcttccgtc caaaacacct gcgtgtgttt
    23221 caggaagcgc gaatgatcca ggcccgcttc ccacgcgcgg ttgccgccct ggatgccgtg
    23281 cggattcacc tcgatcacaa cttccggatt caccgatttc gccagcttcg ccatctggcc
    23341 cagggcatcc gacatcacct ggcaacgaaa atcgatccac tcctgaatcg ccggatcgaa
    23401 tatgatctcc atcctctccg gcggcgtctg gcggttccaa ccgggaggat ttacggggtc
    23461 gatatttgcg aagccgaacc gtgcctggcg ttcggcagcc gtatacttcg tccgaag
//
LOCUS       DASTMK010000585         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_573067, whole genome shotgun sequence.
ACCESSION   DASTMK010000585 DASTMK010000000
VERSION     DASTMK010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573067"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFQ79_24085"
     CDS             complement(<1..899)
                     /locus_tag="VFQ79_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEU0142822.1"
                     /translation="MKRLSLTAWIFIGLAAGVILGVLAPGIAAKLSPVANVFLRLIKS
                     IIAPLIFATLVYGIAGAGNIRAMGRIGLKSIVYFEVVTTVALFLGLAAVNIVKPGEGM
                     RLTRTAEEAAMPQTTTSLSAILEHTFPASIIDAMARGEVLQIVVFALLFGAACASIGV
                     KARPVVEFCESLAEVMFRYTKYVMYLAPFGVGAAMAVTVGTKGFGVLVGLGKLILTMY
                     IAQFIFVVGVLGAVIAIARIPLRRFYQAAREPFLIAFSTASSEAALPRALQNMERFGV
                     PKHIVAFVLPTGYSFNLDGSTLYL"
     gene            complement(896..1759)
                     /locus_tag="VFQ79_24090"
     CDS             complement(896..1759)
                     /locus_tag="VFQ79_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU0142823.1"
                     /translation="MHGIETVGIISKPNVEQAHAVVPGLIAWLKNRKIAVRYDHQTAV
                     YARTNNGISREQVPQGTQLVIVLGGDGTLLSAARAVRGGDIPLFPINLGGLGFLTAIT
                     IDEIYPELERALRGEHRIGRRRMLACQIVRGEEVVGRYEALNDAVITKGGPARIIDLD
                     TYVDSHFVCAYKSDGLIVSTPTGSTAYSLSAGGPIIFPSVGALCITPICPHMLTNRPV
                     IVSDTSVIRVVCLAKDGDAFLTIDGQVGEALKQNDAIICRQAPHTISLIRPPKMLFFD
                     VLREKLKWGTR"
     gene            complement(1746..2483)
                     /locus_tag="VFQ79_24095"
     CDS             complement(1746..2483)
                     /locus_tag="VFQ79_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU0142824.1"
                     /translation="MSAPVPKQRIDQLLVERGFAESREKAKALVLAGQVFVGGARVDK
                     AGQSVPLDSEILIPARLRYVGRGGLKLERALEHFHIGVEARVCIDIGASTGGFTDALL
                     QRGAARVYAIDVGSGQLDWKLRNDSRVVVREKLNARYLVFEDVGEFVDFIACDVSFIS
                     ATLVMPAFVPLLKPDGEFAVLVKPQFEVGRGEVGKGGIVRDPSLHQAACRKVEAEAMR
                     LGFRTDVIESPILGAEGNKEFLLYARH"
     gene            complement(2480..3364)
                     /locus_tag="VFQ79_24100"
     CDS             complement(2480..3364)
                     /locus_tag="VFQ79_24100"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEU0142825.1"
                     /translation="MNIRDYLHEQQKLVDAALDRWVPPVSTPPESIHSAMRYSLFAGG
                     KRIRPILFMEAARTIRGEMAGIEDAACPLELIHTYSLIHDDLPALDNDDLRRGRPTCH
                     KQYGEAMAILAGDALLTLAFQVLVQLPDVDAARKARLVEELAVASGTVGGMIGGQVND
                     LEGEGREPTPTLLDSIHRAKTGALLRASLRMGAIYAQADEDQLAALTCFGEHIGLAFQ
                     IVDDLLDVEESSATLGKTAGKDAQQHKITFPAVYGVARSHEMAEGQRSRALETLERFG
                     PSAGRLRQLADLIVHRRA"
     gene            complement(3361..3612)
                     /gene="xseB"
                     /locus_tag="VFQ79_24105"
     CDS             complement(3361..3612)
                     /gene="xseB"
                     /locus_tag="VFQ79_24105"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU0142826.1"
                     /translation="MPDNSEPQEAVLSFEAGLEQLEAVVKELEGGDLPLDRALELFEQ
                     GMRLSETCRKQLEEAETRVEVLTRKGDRIQAEPFRPEKT"
     gene            complement(3653..>3932)
                     /locus_tag="VFQ79_24110"
     CDS             complement(3653..>3932)
                     /locus_tag="VFQ79_24110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="selenocysteine synthase"
                     /protein_id="HEU0142827.1"
                     /translation="IVDSIASIPTITTEVVVPPIANQVPHLRIQWDPSRVKMSYADVA
                     KELREGDPSIEVTPGTRDALTVTVWMMQPGDAQVVAKRVREVLKKASA"
BASE COUNT          819 a         1211 c         1126 g          776 t
ORIGIN      
        1 agatacagcg tggacccgtc caggttgaag ctgtaccccg tgggcagcac aaaagctacg
       61 atatgtttcg ggacgccgaa ccgttccatg ttctgaaggg cccggggaag agcggcttca
      121 ctcgatgctg tcgagaaggc aatgagaaac ggttctcgcg ccgcctggta gaaacgccgc
      181 agtggaatac gtgcgatagc gatgaccgca ccaagaacgc ctaccacaaa gatgaattgc
      241 gcaatgtaca tggtgaggat gagtttgccg agccccacca gtacgccgaa tcccttggtg
      301 ccaaccgtca ccgccatcgc tgcacctact ccgaaaggag cgagatacat cacgtatttc
      361 gtgtaccgga acatgacttc ggccagcgat tcgcagaact cgacgacggg acgggcctta
      421 acgccgatgg aagcgcacgc cgccccgaac aggagggcga acacgacgat ctgaagcact
      481 tccccgcgcg ccatggcatc aatgatgctc gccggaaacg tgtgttccag aatcgcggac
      541 aagctcgtgg tggtttgcgg catggcggct tcctccgctg tccgcgtcaa ccgcatcccc
      601 tcaccgggct tgacaatgtt gaccgcggcc aatccaagaa acagggctac cgtggtaacg
      661 acttcgaagt aaacgatcga cttcaggccg atgcggccca tcgcacgaat gttccctgcc
      721 ccggcaatgc cataaacgag agtggcgaaa atcaggggtg caataatcga cttgatcaat
      781 cgaaggaaaa cattcgcaac cggcgacaac ttcgcggcga tgccgggtgc aagaaccccc
      841 agaatgacgc ccgcggcaag cccgatgaaa atccaggcgg tgagagagag ccgcttcatc
      901 gagttcccca tttgagtttc tcgcggagaa catcgaagaa aagcatcttg ggcggacgga
      961 tcaggctgat cgtgtgcgga gcttgccggc agataatggc atcgttctgc ttgagagcct
     1021 cacccacctg cccatcgatc gtcaggaatg catctccatc tttggcgagg cagacgacgc
     1081 ggattacgct ggtgtcggag acgatgacag gacgattcgt cagcatgtga gggcaaatcg
     1141 gtgtgatgca aagcgctcca accgagggga agatgatagg tccgccggcc gagagcgaat
     1201 acgctgttga gcctgtcggc gtggagacga tcaggccgtc cgatttatat gcgcaaacaa
     1261 agtggctgtc gacgtaagta tcgaggtcga tgatgcgcgc cggcccgccc ttcgtaatga
     1321 cagcatcatt caacgcctca tagcggccca cgacttcctc cccccggacg atctggcacg
     1381 ccagcatgcg ccggcgtccg atcctatgct caccgcggag ggctctttcc agctcgggat
     1441 agatctcgtc aatcgtaatt gcggtgagga accccagccc accaaggttt atggggaaga
     1501 gcgggatgtc gccgccccgt acggctcttg ccgccgacaa cagcgttccg tcaccgccca
     1561 ggacgataac aagctgcgta ccttgcggaa cctgctcgcg cgatatgccg ttgtttgtgc
     1621 gggcatagac ggccgtttgg tgatcgtagc gcacggcgat tttccggttc ttcagccacg
     1681 caatcaagcc ggggacaacg gcatgggcct gctcgacgtt cggtttggaa ataatgccga
     1741 cggtttcaat gccgtgcata gagcagaaac tccttgtttc cttcggcgcc aagaatcgga
     1801 ctttcgatga cgtccgttcg aaagccgagt cgcattgctt ccgcctcaac cttgcggcag
     1861 gccgcctggt gcaatgacgg atcccgtacg atgcctccct tcccgacttc gccgcgtcct
     1921 acttcgaact gcggcttcac gagcacggcg aactctccgt ccggtttgag cagaggaacg
     1981 aatgcgggca taacgagagt ggctgaaatg aagcttacat cacaagcaat gaagtcgacg
     2041 aactcaccca cgtcctcgaa tacgaggtag cgcgcgttga gcttttcccg tacgacaact
     2101 ctggaatcgt ttctcaattt ccagtccagt tgaccggagc caacgtcaat ggcgtaaacg
     2161 cgcgccgcac cccgctgcag gagggcatcg gtaaagcccc cggtggatgc tccaatatca
     2221 atacacacgc gcgcctcaac gccgatgtga aaatgctcga gcgcgcgttc cagtttgagc
     2281 ccgccgcgcc cgacgtatcg aagcctggcc gggatgagaa tttccgaatc gagcggtaca
     2341 ctctgcccgg ccttatctac ccttgcaccg cccacaaaaa cctgaccggc gaggacgagc
     2401 gctttcgctt tctcccgtga ctccgcgaag ccgcgctcca ccagcaactg gtcgattcgc
     2461 tgtttcggaa ccggggcgct catgccctgc gatggacaat caagtctgcc aattggcgca
     2521 gccggcccgc tgatggtccg aagcgttcga gcgtctccag agcgcggctt ctctgtccct
     2581 ccgccatttc gtgcgagcgt gcaacaccat acacggctgg gaaagtgatc ttatgctgct
     2641 gggcgtcttt gcccgccgtc ttccccaatg tcgctgagga ttcttccacg tcgagaaggt
     2701 cgtcgacaat ctggaacgcc aggccgatat gttcgccaaa gcaggtcagc gcggcgagtt
     2761 gatcttcgtc cgcctgcgcg tagatggcac ccatgcgcaa gctggcccgc agcaacgcgc
     2821 ccgtcttcgc gcggtgaatc gagtcgagca gagtgggtgt gggttcgcgc ccttcgccct
     2881 cgaggtcgtt tacctggcca ccgatcatcc cgcccacggt gccggaggct accgccaact
     2941 cctccaccaa tcgcgccttg cgcgcggcat cgacatccgg caattgcacc agcacctgaa
     3001 acgcgagcgt taacaacgca tcgccggcga ggatcgccat ggcctctccg tattgtttat
     3061 ggcaggtcgg tctgccacgg cgcaggtcgt cattatccag cgcgggaagg tcgtcgtgaa
     3121 ttaacgagta cgtatgaatc agctcgaggg ggcacgcggc gtcttcgatt cccgccatct
     3181 ccccgcggat ggtccgggcg gcctccatga acagaatcgg tcgaatccgt tttccgccgg
     3241 cgaacaagct gtagcgcatc gcgctatgta tgctctcggg gggcgtagaa accggaggca
     3301 cccaccggtc gagagctgca tcgacaagct tctgttgttc gtgcaggtaa tcacgaatgt
     3361 tcatgtcttt tcgggccgga aaggttcggc ctgaatcctg tcgcctttac gagtgagaac
     3421 ttcgacgcgg gtttcggctt cttccaattg cttccggcag gtctcgctga ggcgcatgcc
     3481 ctgctcgaac aactcgagcg cccgatcgag cggcagatct ccgccctcca gttccttgac
     3541 gacggcctcg agttgctcaa gcccggcttc aaagcttagg accgcttcct gcggttcgga
     3601 attgtcgggc atcgcttcaa ttttggcaca cgcgaagccg cggcatcgcc gctcaggccg
     3661 acgccttctt cagaacctcg cgaacccgct ttgccactac ttgcgcgtca cccggctgca
     3721 tcatccagac cgtcaccgtg agcgcgtccc gtgttcccgg ggttacttcg atagaaggat
     3781 cgccctcgcg caattctttc gcgacatcgg cgtaggacat cttcacgcgc gacggatccc
     3841 attggatgcg cagatgcgga acctggttgg cgataggggg aacaaccact tccgtcgtaa
     3901 tcgttggaat tgacgcgatg ctgtccacaa tc
//
LOCUS       DASTMK010000586        15022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_382022, whole genome shotgun sequence.
ACCESSION   DASTMK010000586 DASTMK010000000
VERSION     DASTMK010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15022
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382022"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VFQ79_24115"
     CDS             complement(<1..1353)
                     /locus_tag="VFQ79_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU0142828.1"
                     /translation="MPSDSSFGDILSQFEQEQKTLSGPAQAVEGTVVAIDAESVFVDV
                     GRKIEGVVPVAQFRDKSGELTVKPGDRLTVSISGYDQGYYRLSTVTVERPKDWSSLQT
                     AFSESRTIVGTVTEVVKGGLRVDVGVPAFLPASRSGARDAAEQEALVGQEIQCRIIKL
                     EIAEEDVVVDRRVILEEQEKQARAHAFAAIQEGAVVTGTVRSITDFGAFVDLGGVDGL
                     LHVTDMSWHRVAKPSDVVNVGDSVEVKILKVNPNTRRISVGMKQLVPEPWALVGDKYK
                     TGDRVRGTVSRLTDFGAFVELEPGIDGLIHISELSWSKKVRKPGDVVKVGESVEVAVL
                     GVNPAERRISLGLKQALGDPWEGVPSRYPPGAVVEGTVTSLQKFGAFVDIGDGIEGMI
                     HIGDISREKRLNHPNEMLKMGQSIRAQVLEVDRERRRIRLGMKQLEPTNLDEYIAEHK
                     S"
     gene            1452..2681
                     /locus_tag="VFQ79_24120"
     CDS             1452..2681
                     /locus_tag="VFQ79_24120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0142829.1"
                     /translation="MATNAGTGRGWSRYIRPEDFIWLFLFSAMVVFSPDQTPAEYIFL
                     PCLAIVQIVEPKVPFFGTRRGIHLSIGIKLILSYLLIGFTGAVNSSYYLVLLLPVVTA
                     ATTFGPGGTIAFILLAAASYLSFLLFVDWSRYTIDPYNVRMLCLRLTFISVVGYLTHR
                     LAQMSRIEEGKYQVAAEQLQAAEAAVRRSERLAALGQLSAGLAHELRNPLGTMKASAE
                     LLLKQVPDDNEVAREMAAFIFSEVDRTNGLITRFLEFARPLKLRMKVTDLAEVIDRAV
                     QQVERLRPIAGLTIYKNYSPDVRPLLLDAELFERVVYNLLLNAVQATTPGGAVTVKTR
                     PVDGEVELVVIDRGAGIDAKHLENIFNPFFTTKPQGVGLGLAIVSNIVDEHGGRIAVE
                     SEVGKGSVFIVRLPRRE"
     gene            2727..4088
                     /locus_tag="VFQ79_24125"
     CDS             2727..4088
                     /locus_tag="VFQ79_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0142830.1"
                     /translation="MKPRILIVEDEEKLRRVIELHLLASGFEVDQAGSAEEGLLLADR
                     VQLVITDLRLPGMDGLDLLAQIHRQNAHVPVIVMTAFGTVENAVEAMKSGAADFLLKP
                     FSLNQLTAVVNKALEVRALRDENRQLKEELGHRYQFSNIVGRSSSMQEIFSSITRVAP
                     TRATVLLAGESGVGKDLIARAIHFHSPRAERPFVKINCTAIPENLMESELFGYEKGAF
                     TGAATSKPGKFEQADTGTVFLDEIGDVPASVQVKLLRVLQEREFERLGSNKTRHIDVR
                     VIAATNVDLRAALEQGTFRQDLYYRLNVVPIDLPPLRERKEDIPFLADHFMKKHSREL
                     RGKEARLTDAAVEKLMSYHWPGNVRELENVIERSLVLGSGEFLDAAEIKLDTNPQPRA
                     ASSGAPVLPEGMTLDQLEQEIIRDALERSHGNKSQAARLLGITRNALRYRLSQMGLEN
                     PPE"
     gene            4180..6024
                     /locus_tag="VFQ79_24130"
     CDS             4180..6024
                     /locus_tag="VFQ79_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU0142831.1"
                     /translation="MSTTEITSVVSRSNETAGDVVNDFSIQVATVNGSGSQTANSVLM
                     RSIFQMGIPVSGKNLFPSNIAGLPTWFTIRASKHGWIGRKKEIDFLVAMNPETAHDDV
                     MRLDAGAAVVYDEPLKLNTLRDDLHYYPVPFDKLVAPVCPEAKLRKLVRNMIYDGVVG
                     HLIGVEMEEIHKALFKQFGPKKVKAAELNWNAVQAGRDYAAANLPKTDPCRVARMNKT
                     AGKIIIDGNAAAALGCMFAGVTVVTWYPITPSTSLVESLIGYMRRFRIDKETGKATFA
                     IVQAEDELAAVGMVIGASWAGARAMTATSGPGISLMSEFVGLGYYAEIPAVIFNVQRV
                     GPSTGLPTRTMQGDLLSTALLSHGDTRHPLLFPSSPEECFTMAGTAFDLAERFQTPVF
                     VMLDLDLGMNNWMADAFPYPEQPIDRGKVLTAEDLARLGGFARYKDVDGDGVGYRTLP
                     GTDHPAAGYFTRGSGHNERAGYSERPEDYINNVDRLDKKFETLKEYAPAPEIVIDEQA
                     SIGLICCGTSRYAAEESRDQLAREYGVGSSYLRLKAYPFTDELKDFIARHSRVYVIDQ
                     NRDRQLLGLMRLDLDTQYVERLRSVRYYGGLPLDARTVTDDIVSQEGL"
     gene            6021..7064
                     /locus_tag="VFQ79_24135"
     CDS             6021..7064
                     /locus_tag="VFQ79_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU0142832.1"
                     /translation="MSTTATPPPKKVNRIGLEVLQYRGAKTTLCAGCGHNAITERIIE
                     AFYEMGVEPYKVAKFSGIGCSSKSPAYFLNSAHGFNSVHGRAPAVSTGAMLANHNLIG
                     IVVSGDGDTASIGIGQFMHMLRRNVPLIYIIENNGVYGLTKGQFSATADLGARLKTGI
                     VNDLPAIDTCLAGIELGATLVARSFSGDKRQLLAILKAAIAHKGLSVIDVISPCTTFN
                     DHEGSTKSYSYMKDHDEPLHELDFVPFYQDISVEIAEGEVREVELHDGSKLRLKKLER
                     DYDPTDRIRALDFIHESASSGVVLTGILYVDNKRKTFLDMLNLGDAPLGTLPEAKTRP
                     DQRALDQIMEELR"
     gene            7076..7810
                     /locus_tag="VFQ79_24140"
     CDS             7076..7810
                     /locus_tag="VFQ79_24140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HEU0142833.1"
                     /translation="MPAGPSLENLCVVLVAPRNPLNIGAVARAMSNFGCFALRLVNPY
                     EVAFREARSAVGARSVLERATEHKTLSEAIAGCTLVVGTTALGHRELQHPLRRLEYGG
                     RLIKRHLETGPVALLFGSEKFGLSNEDMSYCHWLMRIPARREHESMNLGQAVAVCLYE
                     LVRDSRAVAKAPKPLKPASAGEMDRFTDELMKLLQESGYVQERTASSSTQKIRRLIRR
                     MGLSARDAEVWLGMIRQIAWKLHSRE"
     gene            complement(7821..8783)
                     /locus_tag="VFQ79_24145"
     CDS             complement(7821..8783)
                     /locus_tag="VFQ79_24145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142834.1"
                     /translation="MASPAPARATSSASITLNLFDGTRRPLRTKAQVLVRIRDGNQND
                     IHSGFHKGSSLVFENLPFYNNFGDNYTVLVSAKGYFQAGFTPVQVAPNIPRVLDLMMI
                     PKNGVFNFRNSTWQRLKSSHPVLSALLAAGAGTDTAARERYEDALENKAAPLACFFNV
                     VTAVRDIRLPSGTPLDYFKQIVWDDPKRAFAQDRFYAWADESMVDQVVRAATQGIFQP
                     EPGAGLFHPGATRSYKQVQFGEANVQLTFHENDRLRVEDTDCVLLEADIDYYKDPLAH
                     ALIEVLKNRTTKSKTDPRSVYVLRWMAGRHAGVPDFDPPYTIEA"
     gene            complement(8956..9831)
                     /locus_tag="VFQ79_24150"
     CDS             complement(8956..9831)
                     /locus_tag="VFQ79_24150"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEU0142835.1"
                     /translation="MPAFVFFLLLLMAPAQREPAASYEQEITRWRARREAALKADGGW
                     LTVTGLFWLKEGANTAGSGPKNTIALPPGSAPGRTGTFLLRKGEVRFEAAPGAGVTLN
                     GKPVSSTTLKADVPGPADVLTIGPLSLILLNRGGKYAIRLKDNNSSFRKEFTGLHWYP
                     VKREYRIVATFVPYAQPKTISIPTLLGTSEEQHSPGYAEFALDGTTHRLEPVTEGDEL
                     FFIFRDLTSRKQTYPAGRFLYSAQPENGRVVLDFNKAYNPPCAFTPYATCPLPPPQNR
                     LRVKIEAGELNYGHH"
     gene            9897..11078
                     /locus_tag="VFQ79_24155"
     CDS             9897..11078
                     /locus_tag="VFQ79_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142836.1"
                     /translation="MSENNRRNFLGSSATAAGVLILKPETVFGTQANSALEVGIIGCG
                     GRGNFIGGFFIEYTGARISALADPFQDRMESTREKLRASGARLFPGLNGYKELIASNV
                     DAVAVMSPPYFHPEQVAAAVAAGKHVFLAKPVAVDVPGCRSILASGEKAKGKLSFVVD
                     FQTRVQPVFKEAAGRVHRGDIGTPVLGHVYYHAGRLRPKTEPGMSAAQARLSNWVFDK
                     VLSGDIIVEQNVHVLDAGNWYLKLHPVKASGTGGRKARVDVGDCWDHFLVNFWYPNDV
                     KVDFSSAQFTKGYNDLCIRIYGSGGTADTHYNGTVRITGDKPWEGGEKDDTFRQGAIT
                     NVRNFAASVRSGDYLNNAQESVESNLTAILGRMAAYRESAVTWDEMMRSNEKLEANLK
                     L"
     gene            complement(11084..12253)
                     /locus_tag="VFQ79_24160"
     CDS             complement(11084..12253)
                     /locus_tag="VFQ79_24160"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142837.1"
                     /translation="MTRRDAARAAGLTAVSYSRVRGANDRIGLGVIGTGGRGTYVMTL
                     FQKNPDVEVRAICDVYGKRIDQALGRAPGAKTFIDHRELLRLKEVDAVLIGSPDHWHK
                     DHSIDAMTAGKDVYVEKPMCRLREEAPLMVRAARANNRICQIGLQQRSGPIYIEAREK
                     FVRSGALGRITQVDAIWHGGVPRPMLKEPAEKPSNLDWIRFLGPVRYRDWVPGQYFNF
                     RAYLDFNGGKITDFGHHWLDTVHMYMGERAPRSAVFAGGIYYDFNDGRTAPDTCNALF
                     EYDGFSVLFQSNVYRGEYGITFHGDQGRLFVNRNRYEFTPAGKGAQPVVREIPGDITA
                     DHVRNFLDCCKSRKLPAADAAVAAISIIPPLLAVESYVTKRKLVFDPERLEVLPD"
     gene            12479..13696
                     /locus_tag="VFQ79_24165"
     CDS             12479..13696
                     /locus_tag="VFQ79_24165"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0142838.1"
                     /translation="MSISQLARSISESPTLKLNETAALLRDKGEPVIHLGGGEPKSKT
                     PIDAIISCSSLLNTGEVKYTPADGIPALKKAVIHYTEEHYDKRVAPENVIVSSGAKQA
                     IMVALYAILDPKDEVVFPAPYWVSYPEMVKLAGGVPVPVCAKDGTFFPSIGDITEVLS
                     PYTKAIILNSPNNPSGVMYSKEFIAEVVALCESRNIYLIMDDTYNRLIFDGRTPVNCY
                     EFTEEDVETSKLIVINCVSKMYAMTGFRIGWAVAPRELIRAMTNIQSQQTSGPAAPSQ
                     WAAVGALNGVQASIEALRLTLENNRNVMVDRLSAIDGVSVVKPDGTFYCFPDFSAYEK
                     NSQKLSQFLLEKVRVVTVPGKEFGMEGHLRLSFCGTIKEIREGIERIQWALDSNSANE
                     LYLGNRKLVRDWV"
     gene            13693..>15022
                     /locus_tag="VFQ79_24170"
     CDS             13693..>15022
                     /locus_tag="VFQ79_24170"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU0142839.1"
                     /translation="MSWYMNVPTPAAKEAGLAASTYGLENHGLTHLHNTYWNLPTASL
                     YEEIAFRGEGRVAHLGPMVVNTGKHTARAAADKFIVREQTTEEKVWWGEYNRPFSPQN
                     FNALQVRLQGYLQGRDVFVQDCFAGADPEYRIPIRIITELAWHSLFVRNMFIKSRSLD
                     AAKKHVPEFTVICVPSFRASPLVDGTRTDTFIIGNFAQRTAIIGGSRYGGEIKKTIFT
                     VLNYLLPLQGVLSMHCSANVGPDGDSALFFGLSGTGKTTLSADPDRNLIGDDEHGWSD
                     NGIFNFEDGCYAKVIRLSRHAEPQIYECTRRFGTLLENVPMDPRTRRLDLNDDKITEN
                     TRAAYPLDYIGNSLPEKMAGHPKNIILLTCDASGVMPPIARLTPEQAIYHFISGYTSK
                     IAGTEIGLGTEPEITFSTCFGAPFMVHRPEVYAQLLHARILKHNVSCWLVN"
BASE COUNT         3108 a         4383 c         4425 g         3106 t
ORIGIN      
        1 ggacttatgt tccgctatgt actcgtcgag gttcgtgggt tccagttgtt tcatgccgag
       61 gcggatgcgc cgtctctcgc ggtcgacctc cagcacctgc gcgcggatgc tctggcccat
      121 cttcagcatt tcgttcggat gattcagccg tttctcccgg ctgatgtcac cgatgtgtat
      181 catcccttcg atgccatcgc cgatatccac gaacgcgccg aacttctgaa ggctggtgac
      241 ggtgccctcc acaaccgctc cgggcggata ccggcttgga acgccttccc agggatcgcc
      301 aagcgcctgt ttcaacccca gcgaaatacg ccgttccgcc ggattgactc ccagaaccgc
      361 aacttcgacc gactcgccta cctttactac atcaccgggc ttgcgtacct ttttcgacca
      421 ggacaactcc gagatatgaa tcaggccatc gatgcccggc tcgagttcga cgaacgctcc
      481 gaagtccgtc agccgcgata cggtgccgcg cacgcgatcg cccgttttgt acttgtcgcc
      541 caccagcgcc cagggttcgg gcacaagttg cttcatcccg acagaaatcc tgcgcgtatt
      601 gggattcacc ttgaggattt tcacctcgac tgaatcgccc acatttacga cgtcggacgg
      661 cttcgcgacg cgatgccacg acatgtcggt gacatgcagc agtccatcga caccacccaa
      721 atcgacgaat gctccgaaat cggtgatact gcgcacggtg ccggtgacaa ccgccccttc
      781 ctgaatcgcc gcaaaggcat gggcgcgcgc ttgcttttcc tgttcttcga ggatgacgcg
      841 gcggtccacc accacgtctt cctcggcgat ttccagcttg ataatccggc actggatttc
      901 ctgcccgacc aacgcctctt gttccgcggc gtccctggcc ccgctacgcg atgccggaag
      961 aaatgcgggc acacctacat ccacccgcag tccacccttc acaacctccg tcacggttcc
     1021 cacgattgtc cggctttcgc tgaaagcggt ctgaagggac gaccagtcct tcgggcgctc
     1081 aacggtgacc gtcgagagcc tgtagtatcc ctgatcgtat ccgctgatac tgaccgtcaa
     1141 ccggtccccc ggctttaccg tcagctcgcc ggacttgtca cggaattgtg caaccggcac
     1201 aactccctcg attttgcggc cgacatcgac gaaaacagat tcggcatcga tggcaacgac
     1261 agtgccttca accgcttgag ccggaccgga gagggttttc tgttcctgct caaattgaga
     1321 caggatgtcg ccaaaagagc tgtcagaagg cacagattgt tcctctggcg tggcagatac
     1381 ggggtaggga tgtttcattg ctgctggggc ttgaaaccct attttcacat attcggggct
     1441 aagatggcag cgtggccacg aatgcgggga ccgggcgcgg ctggtcccgt tatatcagac
     1501 ccgaggattt catctggctg ttcctgtttt ccgcgatggt ggtcttcagc ccggatcaaa
     1561 cccccgccga atacatcttt ctcccatgtc tggcgattgt gcagattgta gagccgaaag
     1621 tgccgttttt cgggacacgc cgcggcattc acctgtccat cggcataaag ctgatcctga
     1681 gttacctgct catcgggttt accggcgctg tgaacagcag ctattatctg gtgctgctgc
     1741 tgccggtggt gaccgccgca accacattcg gtccaggcgg taccatcgct ttcatcctgc
     1801 tggcggcagc gtcgtatctc tcctttctgc tgttcgtgga ctggagccgt tacacaattg
     1861 atccctataa cgtgcggatg ctctgtctgc ggctcacgtt catcagcgtc gtcggctatc
     1921 ttacccatag acttgcccag atgagccgca tcgaagaggg caaataccag gtggcggcag
     1981 agcagttgca ggcggctgaa gcggcggtcc gccgctccga gcgtctggcc gctctggggc
     2041 aattgtccgc cggacttgca cacgagctgc ggaacccgct tggcacgatg aaggcatccg
     2101 ccgaactgtt gctcaagcag gtacccgacg ataacgaggt ggcgcgagaa atggcagcct
     2161 ttattttttc cgaagtcgac cgtaccaacg gcctcatcac acgctttctc gagttcgccc
     2221 gtcccctgaa attgcggatg aaagtaacag accttgccga ggtgattgac cgcgccgtcc
     2281 aacaggtcga acgcctgcgt ccaatcgccg gcctgacaat ctataaaaac tattcgcccg
     2341 atgtccgccc gctgctgctc gacgccgagc ttttcgaacg ggttgtgtac aacctgctgt
     2401 tgaatgccgt gcaggcgacc acgcctgggg gagccgtcac ggtgaaaacg cggccggtag
     2461 acggtgaggt ggaacttgtg gtgatcgacc gcggcgccgg catcgacgcc aaacacctgg
     2521 agaacatttt caaccccttc tttacgacaa agccgcaagg cgtgggcctg gggctggcta
     2581 tcgtttccaa catcgtcgac gaacatggag gccgaatcgc ggtggagagc gaagtgggca
     2641 aggggagcgt ttttatcgtg cgcctgccgc gccgcgaata ggacgcgttt gggcctcaac
     2701 tgcctgtctc agtattctaa ggatcagtga agccccgcat actgattgtt gaagacgaag
     2761 agaagctgcg gcgtgtcatc gagttgcacc tgcttgcgtc cggtttcgaa gtggaccagg
     2821 ccggaagcgc tgaggaaggg ctgctgctgg ccgaccgggt ccagcttgta attaccgatt
     2881 tgcgtctgcc cgggatggac gggctcgatc tcctggcgca gatccacagg caaaatgcgc
     2941 acgtgccggt gattgtaatg accgccttcg gcactgtcga aaacgcggtg gaagcgatga
     3001 agtccggcgc tgcggatttt cttctgaagc ctttttcact caaccagctt acggctgtcg
     3061 ttaacaaggc tctcgaggtc cgcgcgctca gagatgagaa ccggcagttg aaggaggaac
     3121 tcgggcatcg ataccagttc agcaacatcg ttggccgtag ttcctccatg caggagatct
     3181 tcagctcgat cacgcgcgtg gctcccactc gcgccacggt gctgctggca ggtgaaagcg
     3241 gcgtcgggaa ggatctgatc gcgcgcgcga ttcacttcca ttccccacgg gccgaacgtc
     3301 cgtttgtgaa gatcaactgt actgccatac cggagaatct tatggaaagt gagctcttcg
     3361 gttatgagaa aggggctttc acgggggccg cgacttccaa gcccggcaaa tttgaacagg
     3421 cggacacggg aaccgtattt ctggacgaga ttggggatgt gcctgcctcg gtgcaggtaa
     3481 agctgcttcg agtgcttcag gagcgcgagt tcgaaagact gggcagtaac aagacgcgcc
     3541 acatcgacgt gcgtgtgatc gcagcgacga acgtcgatct gcgggccgcg ctggagcagg
     3601 gcacatttcg ccaggatctc tactaccgct tgaacgtggt gcccatcgat cttccaccat
     3661 tgagggagcg taaagaagac atcccgtttc tcgccgatca tttcatgaag aagcattccc
     3721 gcgaattgcg cggtaaggag gcgcgtctta ccgatgccgc ggtcgaaaag ctgatgtcct
     3781 atcactggcc gggcaacgtc cgcgagttgg agaatgttat cgaaagaagt cttgtattgg
     3841 gcagcggaga gtttctcgac gcggcggaaa tcaaactcga cacgaacccg cagccgcgag
     3901 cggcttcttc cggagcgccg gtcctgcccg aaggcatgac cctcgaccaa ctcgaacagg
     3961 aaatcattcg cgatgcactc gaacgttcac acggtaacaa gagccaggcg gcgcggttgc
     4021 tcgggatcac caggaatgcg ctgcgttacc ggctttcgca gatggggctg gagaatccgc
     4081 ccgagtaaac ccctgcctgc gtatcggaca gaaacatcct aaaatcgaag cttttcggta
     4141 tcttctatat aatgcgggta gtgaaggtat agattccaaa tgtcgacgac cgagattact
     4201 tctgttgtct cgcgttccaa cgagacggcg ggagatgtag ttaacgattt cagcattcag
     4261 gttgcgacgg taaacggctc ggggagccag accgccaaca gtgtgctgat gcgctccatc
     4321 tttcagatgg gaattccggt ttccggaaag aacctgttcc cctccaatat cgccgggttg
     4381 cccacctggt tcacgattcg cgccagcaag cacggctgga ttggacgcaa gaaggagatc
     4441 gatttcctgg ttgcgatgaa tccggaaacg gctcatgacg atgttatgcg gctggacgcc
     4501 ggcgcggccg tagtgtatga cgaaccgttg aagctgaata cactccggga cgacctgcat
     4561 tattatccgg ttcccttcga taaacttgtc gctcctgttt gccccgaggc gaagctgcgc
     4621 aaactggtgc gaaacatgat ctacgacggc gttgtcgggc acctgatcgg tgtagagatg
     4681 gaggagatcc acaaggcgct gttcaagcag ttcgggccga agaaggtcaa ggcggccgaa
     4741 ctcaactgga atgcggttca ggccggtcgc gactacgccg ccgccaacct ccccaagacg
     4801 gatccgtgcc gtgttgcgcg gatgaacaag accgcgggca agatcatcat cgacggcaat
     4861 gcggcggcgg cgctgggttg catgttcgcc ggagttacgg ttgtgacgtg gtatcccatc
     4921 actccttcca cgtcgttggt cgaatcgctc atcggctaca tgcgccgctt ccgtatcgac
     4981 aaggagaccg gaaaagccac tttcgcgatc gttcaagccg aagatgagct ggcagcggtt
     5041 ggaatggtga tcggcgcctc gtgggccggc gcccgcgcga tgacggcgac ctccggtccg
     5101 ggcatttccc tgatgtccga gttcgttggt ctcggctatt atgccgaaat cccagccgtt
     5161 atttttaacg tgcagcgcgt ggggccgtcc acaggcctgc cgacgcgaac gatgcagggg
     5221 gacctgctct ccaccgcatt gctgtctcac ggggacacac ggcacccgct gttgtttccg
     5281 tcgtcgccgg aagagtgctt caccatggcg gggacggcgt tcgatttagc cgagcgattc
     5341 cagacacctg ttttcgtgat gctggatctc gatctcggca tgaacaactg gatggcggat
     5401 gcattcccgt atcccgagca gcccatcgac cgcggcaaag tgctcacggc cgaggacctt
     5461 gcgcggctgg gaggttttgc ccgttataag gacgtggatg gcgacggggt gggctaccgc
     5521 acgctgcccg gcaccgatca tccggcggcg ggctatttca cccgcggcag cggccataac
     5581 gagcgcgccg gctatagcga gcggcccgaa gactacatca acaacgtgga ccggctcgac
     5641 aagaagttcg aaactctgaa agagtacgcc ccggctcccg aaatcgtcat tgacgaacaa
     5701 gcgtctattg gcctgatttg ctgcggcacc tcccgctacg ccgcggaaga gagccgcgat
     5761 caactggcgc gggaatatgg tgtcgggagt tcctatttgc gcctgaaggc atatccgttt
     5821 acggacgaat tgaaggactt catcgcgcgg cattcccgtg tttacgtgat cgatcagaat
     5881 cgcgaccgcc agttgttggg attgatgcgg ttagacctgg acacgcagta cgtagagcgg
     5941 ctgcgaagtg tccgctatta cggtggcctg ccgctcgacg cgcgcacggt aaccgacgac
     6001 atcgttagcc aggagggctt atgagcacca cagcgacgcc tcctccgaag aaggtcaacc
     6061 ggatcggact cgaggttctt cagtacaggg gcgcgaagac gacactgtgc gccggctgcg
     6121 gccacaacgc gatcacggag cgcatcatcg aggcgttcta cgagatgggt gtagagccgt
     6181 ataaggttgc aaaattttcg ggcatcggct gctcgtcgaa atcgccggcc tatttcctga
     6241 attccgcgca cggcttcaac tccgtccatg gacgcgcgcc ggcggtgagc acgggagcga
     6301 tgttggcgaa tcacaacctg atcggcatcg tggtttccgg cgacggcgat accgcctcga
     6361 tcggtatcgg ccagttcatg cacatgctgc gccggaacgt ccccctgatc tatatcatcg
     6421 agaacaatgg cgtctacgga ttgaccaagg ggcagttctc ggcgacggcc gatctcggcg
     6481 ccaggctcaa gacaggtatc gtcaacgatc ttccggccat cgacacctgc ctcgccggca
     6541 tcgaattggg tgcaactctt gttgcgaggt ccttctccgg cgacaaacgg cagttgctcg
     6601 cgatcctgaa ggccgcaatc gcccacaaag gattgtcggt catcgatgtg atttcgcctt
     6661 gcacgacatt caacgatcac gaggggtcca cgaagagcta ttcctatatg aaagatcacg
     6721 acgagcctct gcatgaactg gacttcgtgc cgttctacca ggacatttcg gtcgagatcg
     6781 ccgaaggaga ggtgcgcgag gtcgaattac acgacggctc caagttgcgt ttgaagaagc
     6841 tggaacggga ctacgatccc accgacagga ttcgcgcgct cgactttatc catgagagcg
     6901 cttcgtccgg tgtcgtactg accggaattc tctacgtaga caacaagcgg aaaacctttc
     6961 tggacatgct caatctgggg gatgcccccc tgggtacgct acccgaagcg aagacccggc
     7021 cggatcaaag agctctcgac cagatcatgg aagaactcag ataggaaccg cccacgtgcc
     7081 cgcggggccg tcgctcgaaa acctgtgcgt ggtgctggtt gcaccgcgta atccattgaa
     7141 tatcggggcg gtggcgcgcg caatgagtaa tttcgggtgc ttcgctctgc ggctggtcaa
     7201 cccctacgag gtcgcgtttc gcgaagcccg gtcggcggtg ggagcgcgca gtgtactgga
     7261 acgggcgacg gagcacaaga cgctctccga ggcaatcgcc ggctgcacgt tggttgtggg
     7321 caccacagcc ctcgggcacc gcgagcttca gcatccgttg aggcgcctgg agtacggcgg
     7381 aaggctcatc aagcggcacc tggagaccgg ccccgtcgct ctactcttcg gctcggaaaa
     7441 attcgggttg tccaatgagg acatgagcta ctgccactgg cttatgcgca taccggcacg
     7501 gcgggagcat gaatcgatga acctcggtca ggccgttgcc gtctgcctct acgagctggt
     7561 tcgagattcg cgtgccgtag ccaaagcgcc gaagccgttg aagcccgcga gtgccggtga
     7621 aatggaccgc tttaccgatg agctgatgaa gttgcttcag gagtccggat acgttcagga
     7681 gcgcacggcg agctccagca cacagaagat ccgcagatta atacgaagaa tgggactttc
     7741 ggcccgtgat gccgaagttt ggctcggcat gattcggcag atcgcctgga agctgcactc
     7801 ccgggaatag caggcttcta tcaggcctcg atggtgtatg gcgggtcgaa atcgggaaca
     7861 ccggcgtgac ggccggccat ccagcgcagc acgtacaccg atctcggatc tgttttgctt
     7921 tttgtcgtgc ggtttttcaa tacctcgatg agggcgtgcg caaggggatc tttgtagtag
     7981 tcgatatcgg cttccagcag aacgcagtcc gtatcttcga ccctcagccg gtcgttctca
     8041 tggaacgtga gctggacgtt cgcttctcca aactgcacct gtttatagct gcgcgtcgct
     8101 cccggatgaa atagtcccgc accgggttcg ggctggaaga tcccttgcgt cgcggcgcgc
     8161 actacctgat ctaccatgga ttcgtccgcc catgcgtaga accggtcttg agcgaacgcc
     8221 cgcttgggat cgtcccaaac gatctgcttg aaataatcca gaggggttcc cgaaggcagc
     8281 cggatatccc ggacggcggt gacaacgtta aaaaagcagg cgagcggtgc ggccttgttc
     8341 tccagtgcgt cttcgtatct ctcccgcgcc gccgtatcgg ttcccgcacc ggcggcgagc
     8401 agtgccgaaa gaaccgggtg actcgacttc agccgttgcc aggtagagtt ccggaagttg
     8461 aagacaccat ttttaggaat catcatcaag tcgaggactc gcggaatatt cggtgccacc
     8521 tgtaccggag tgaagcccgc ctggaaatac cccttggccg agaccagaac cgtgtagtta
     8581 tcgccaaagt tgttatagaa ggggagattc tcgaacacca gtgacgatcc cttgtggaat
     8641 ccgctatgga tgtcattctg gttgccgtcg cgtatccgga ccagcacctg ggctttggtc
     8701 cggagcggcc gccgcgtgcc gtcaaaaagg ttcagtgtga tggaagcgct gctcgtggcc
     8761 cgggcgggcg cgggactcgc catgttcttc tcctctttcg ataactggac tgtcgagtga
     8821 taccatagct cacccgcctt gtcaaaaatt caccgggccg ttaccggatt tccaatcggt
     8881 tgttatgaac tgctctttaa aatacaattt atacgcaaat cgtcgctgtt cggtgcagcg
     8941 attgagcccc ccgctttaat ggtggccgta gttcaattca ccagcctcga tcttcacccg
     9001 cagccggttt tgcggcggcg gcagcgggca agtcgcgtaa ggagtaaatg cgcacggcgg
     9061 gttgtacgct ttattgaagt cgagcaccac gcggccgttt tccggctgtg ccgaatagag
     9121 gaaacggcct gccggatagg tctgtttgcg gctggtcagg tcccgaaaga tgaagaacag
     9181 ctcgtcccct tcggttacgg gctccagccg gtgcgtcgta ccgtcgaggg caaactccgc
     9241 atagccaggc gaatgctgtt cttcgctggt cccgagtagg gttggaattg aaatcgtctt
     9301 cggttgcgca tagggcacaa acgtggcaac aatccggtac tcacgcttca cgggatacca
     9361 atggaggccg gtgaattctt ttcggaacga gctgttattg tccttgagcc ggatggcata
     9421 cttaccgccc ctgttcagca ggatgagcga cagaggccct atcgtgagaa cgtctgccgg
     9481 tccaggcacg tctgccttca gcgtggtact actgaccggc ttaccgttca gggtcacacc
     9541 cgctccggga gcggcctcga accgcacttc ccctttgcgc agcaggaatg tgccggtgcg
     9601 gccgggcgcg gaaccgggtg gaagggcgat cgtgttcttc gggccgctgc cggccgtgtt
     9661 tgcaccctct ttcagccaga acagtccggt cacggtcagc caaccgccat cggctttgag
     9721 cgcggcttcc cgccgcgcgc gccaacgggt gatctcctgc tcgtaactgg cggcaggttc
     9781 acgttgcgcg ggcgccatca acaaaagcag gaagaagacg aaggccggca ctgcctctat
     9841 tatgaggccc ccaacgggcg tgcgcatttg ctgtaaaatc gcagccggag atgtaaatgt
     9901 ccgaaaacaa ccgacgcaat tttctgggat cttccgccac cgccgcgggt gtcctgattc
     9961 tgaagcctga aaccgtattt ggaacgcagg cgaattccgc gctcgaagtt ggcatcatcg
    10021 ggtgcggggg ccgcggcaat tttatcggcg ggttctttat cgagtacacg ggcgcccgta
    10081 tttcggccct tgccgatccc ttccaggatc gaatggagtc tacacgggag aaattgcggg
    10141 caagcggcgc ccgcctgttt cccggcttga acgggtacaa agagttgatc gcttccaatg
    10201 tggatgcggt cgcggtgatg agccctccat acttccatcc ggagcaggtg gcggcggcgg
    10261 tcgccgcagg caagcacgtg tttctggcga agcctgtggc tgtcgatgtg cccggctgcc
    10321 gcagtattct cgcgagtggt gaaaaagcca aaggcaagct gagcttcgtc gtcgatttcc
    10381 aaactcgcgt ccagccggtg ttcaaggaag cggccggccg cgtgcatcgc ggggacatcg
    10441 gaacgccggt actgggccat gtctactatc acgcggggcg gctcaggccg aagaccgaac
    10501 ccggtatgtc cgccgcgcag gcgcgactga gcaactgggt gttcgacaag gttctttccg
    10561 gcgacattat cgtggagcag aacgtacatg tcttggatgc cggaaactgg tatctcaagt
    10621 tgcatccggt gaaagcgtcc ggaactggtg ggcgcaaagc gagggtggat gtcggcgact
    10681 gctgggacca cttcctggtg aacttctggt acccgaacga cgtcaaggtc gacttcagct
    10741 ctgcccagtt caccaaaggg tacaacgatc tctgtattcg catctacggt tcgggcggca
    10801 cggccgatac ccactataac ggcacggtcc gcatcaccgg agacaagccc tgggaaggcg
    10861 gggagaagga cgatactttc cggcagggcg cgatcacgaa cgtacgtaat ttcgccgcca
    10921 gcgtacgcag cggcgactac ctgaacaacg cccaggagag cgtggagagt aatttgacag
    10981 cgatcctcgg gcgcatggcc gcctaccgcg aatccgccgt aacgtgggac gagatgatgc
    11041 ggtcgaacga gaagctggaa gcgaacctga agttgtgagg cactcagtcc ggaagaactt
    11101 cgagtctctc cggatcgaac acgagtttcc gcttcgtcac atacgactcc acggccagca
    11161 gcggcgggat gatagagatc gccgcgaccg ccgcatcggc cgcaggcagt ttgcgggact
    11221 tgcagcaatc gaggaagttg cggacatggt ccgccgtgat gtcgccgggg atttcccgca
    11281 cgaccggctg cgcccccttt cccgcgggcg tgaactcata ccggttgcgg ttcacgaaga
    11341 gcctcccctg gtcgccatgg aaggtgatcc cgtattcgcc ccggtaaaca ttcgattgaa
    11401 agagcacgct gaaaccgtcg tactcaaata gcgcgttgca ggtgtccgga gccgtgcggc
    11461 catcgttgaa gtcgtagtag atacccccgg cgaagacggc tgaacgtggg gcgcgctcgc
    11521 ccatgtacat gtgcacggtg tccagccaat gatggccgaa atcggttatc tttccgccgt
    11581 tgaaatcgag ataggcgcgg aaattgaaat actggcccgg cacccagtcc cggtagcgga
    11641 cgggacccag gaaccggatc caatcgagat tcgaaggctt ttccgcgggt tccttgagca
    11701 tcgggcgcgg cacgcctcca tgccatatgg cgtcgacttg ggtgatccgg cctagcgctc
    11761 cggaacggac gaacttctct cgtgcctcga tgtaaatagg cccggagcgc tgttgcagcc
    11821 cgatctggca gatacggttg ttcgcccggg ccgcgcgcac catcagcggg gcctcttccc
    11881 gcaagcggca catcggcttc tccacgtaca cgtccttgcc tgcggtcatg gcatcaatcg
    11941 aatggtcttt atgccagtga tccggcgacc cgatgagcac cgcatccact tccttgaggc
    12001 gtagaagctc gcgatgatcg atgaaggttt tcgcgcccgg agccctgccg agtgcctggt
    12061 cgatgcgctt gccgtatacg tcgcagatgg cgcgcacctc cacgtccgga ttcttctgaa
    12121 acagcgtcat gacatacgtg ccgcggccgc cggtcccgat cacgccgagt ccgatccggt
    12181 cgttggctcc gcgaacccgc gaataggaga ctgccgtcag tccggccgcc cgggccgcat
    12241 cgcgccgtgt catgatactc atcttctcac catggaggag cgttgttcac aaagtcttgc
    12301 cttaaatcct tcttgagtcc gtgttcccga gtgtaatatc attccagaaa ggtaatcttt
    12361 cagatctgta agtctgcttt gcaagcagag actaccttcc gttgtaggcg gcgccagcgc
    12421 cttgtactgg cgatagttgt atccgaactt ggagacgtag gaaggaagta tcaaccgaat
    12481 gagcatcagc cagctagccc ggtcgatcag cgaatcgccc accctgaagc tgaacgaaac
    12541 cgcagcgctt ctcagggaca aaggcgagcc agtcatacat ctcggcggcg gagagccgaa
    12601 gagtaaaact cctatcgacg caatcataag ctgttcctct ctgctgaaca cgggagaagt
    12661 caaatacaca ccggcggacg gtatacccgc tctcaaaaaa gccgtcatcc attacacgga
    12721 agaacactac gataagcgtg tcgcaccgga aaacgtaatt gtttccagcg gggccaagca
    12781 ggccatcatg gtggcgctct atgccattct cgatccgaag gacgaggtcg tgttccccgc
    12841 gccctattgg gtgagctacc ccgaaatggt gaaactggcc ggtggcgtgc cggtccccgt
    12901 atgcgccaag gacggcactt ttttcccgag catcggggac attacggaag ttctcagccc
    12961 ctacaccaag gccatcatcc tgaacagccc gaacaacccc agcggcgtca tgtactcgaa
    13021 ggagttcatt gccgaagttg tcgccctgtg cgaatcgcgc aacatctacc tgatcatgga
    13081 tgatacctac aaccggctga ttttcgatgg caggacgccc gtaaactgct acgagtttac
    13141 ggaggaggat gtcgagacgt cgaagctgat cgtcatcaat tgcgtttcca agatgtacgc
    13201 catgaccggg ttccgcattg gatgggccgt cgctccgcgg gagttgatcc gggcgatgac
    13261 caatattcaa tctcagcaga cctccggacc cgccgcccct tctcaatggg ctgccgtcgg
    13321 agcgctgaac ggagtacagg cgtccattga agcattgcgg ctcaccctcg aaaataatcg
    13381 caatgtcatg gtcgaccggc tgagcgccat cgacggcgtc agcgttgtca aaccggatgg
    13441 caccttctac tgcttccccg atttcagcgc gtacgaaaag aattcgcaaa agctgtcgca
    13501 gtttttgctc gaaaaggttc gcgttgtaac cgtgccggga aaagagttcg ggatggaggg
    13561 tcacctccgc ttgagcttct gcggaacgat caaagaaatt cgcgaaggca tcgagcgtat
    13621 tcaatgggcg ctcgactcga actcggctaa cgaactgtac ctgggcaacc gcaagcttgt
    13681 gagggattgg gtatgagctg gtatatgaac gttccgacac ccgccgccaa ggaagccgga
    13741 ctcgcggcca gtacttacgg actcgagaac cacggcctta cgcacctgca taacacctac
    13801 tggaatctgc cgacggcgtc tctttacgaa gagatcgcct ttcgtggcga gggacgggtt
    13861 gcccaccttg gaccgatggt tgtgaacacc ggaaagcaca cggcgcgggc agccgccgac
    13921 aagttcatcg tgcgagagca gacgacggaa gaaaaggtgt ggtggggaga atacaatcgc
    13981 ccgttcagcc cgcagaactt taacgcgctc caggtgcgtc tgcagggcta cctacagggc
    14041 cgcgacgtat tcgtgcagga ttgtttcgcc ggagcagacc ctgaatatcg catacccatc
    14101 cgcattatca cggagcttgc ctggcacagc ctgtttgtgc gaaacatgtt tatcaagtcg
    14161 cgcagcctgg acgcggcgaa gaagcatgtc cccgagttca cggtgatatg cgtgccttcc
    14221 ttccgcgctt cgccgctcgt ggacggcaca cgcaccgata catttatcat cggcaacttt
    14281 gcccagcgga ccgccattat cgggggctcc cgttacggcg gcgagatcaa gaagaccatc
    14341 tttacggtgc tgaattacct tctgcctttg cagggggtgc tttccatgca ctgctccgcg
    14401 aacgttgggc cggacggcga ttcggcgctg ttcttcgggt tgtcgggaac gggaaagact
    14461 acattatccg ccgacccgga ccgaaatctg atcggcgacg acgagcacgg ctggagcgat
    14521 aacgggattt tcaatttcga agacggctgt tacgcaaaag tgatccggct ctcgcgccac
    14581 gcggagccgc aaatctacga atgcacgcgc aggttcggca ctcttcttga aaacgttccc
    14641 atggatccgc ggacccgccg gcttgacctc aacgacgaca agatcaccga aaacactcgc
    14701 gccgcatacc cgctggatta catcgggaac tcgctgccgg agaagatggc cggtcacccg
    14761 aagaacatca tccttctgac gtgcgacgcc tccggggtga tgccgcccat cgccaggctc
    14821 actcccgaac aggccatcta tcatttcata tcggggtata caagtaagat cgcggggacc
    14881 gagataggat tgggcactga acccgagatt acgttcagca cttgcttcgg agcgccgttt
    14941 atggttcacc gcccggaagt ctacgcacaa ctgctgcatg cgagaatcct gaagcacaat
    15001 gtcagttgct ggctggtcaa ta
//
LOCUS       DASTMK010000587         5949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_157255, whole genome shotgun sequence.
ACCESSION   DASTMK010000587 DASTMK010000000
VERSION     DASTMK010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157255"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2876
                     /locus_tag="VFQ79_24175"
     CDS             <1..2876
                     /locus_tag="VFQ79_24175"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0142840.1"
                     /translation="IQFVLEKDPEVAAQEVRDKVNTILGDLPRDADPPIIEKVATDAA
                     PVISIVVASGRDLRETTKVVDDRIKKNIEGLSGVGQVRFVGERQRQIQVWLDGEKLYA
                     YNLNIDQIRAALAAQNVEIPGGRVDQGNRELSLRTLGRVEHPKDFERIIVGNIGGSPV
                     RISDIGYVVDGVEEPRSLARLNGTSAVVLEVRKQAGTNTVEVINNIKERIAELKSTLP
                     PDFEITYTRDTSTFIEGSFHAVQEHLLLGGFLAAIIVLVFIRSWRSTLIAAVAIPTSI
                     ISTYSLMSWMGFSLNQITMLALTLMVGIVIDDAIVVLENIFRFMEEKGLPPVEAAIQG
                     TRDIGMAVMATTLSLVIIFLPVALMGGIVGRFMSSFGYTAAFAIMVSLLVSFTLTPML
                     CARFLKPPEKKEGEGEVPGSSTKDTWVFRYMASGYWKLLHWSMNHRKIVAFVSLLVVV
                     STVPLFMAVGKDFLPTDDQSEFEVTVRMPAGSSLAGTEALMRELEAELKTLPGVQDLL
                     TTIGADTRRQVDRGSILVELLPMDERTHSQQELMSMAREKLRKFRELVIAVQLPSLIQ
                     GAGPNQDFQFFIQGPDLEQIDKYAQQLKQRLAEVPGVADLDSSYEGGKPELRVNINRD
                     KAADLDVSVSSVATALRTLVGGDEQVTTYREGDDRYDVQLRVREEFRNSPRALERLYV
                     PSGTLGNVPISNVASLKESIGPTQIERYNRQRQIMVSANVVEGQSLSNVLQIVDRTVA
                     DMNLPAEYRTGLLGQSKEFGRAFTNYVIAFLLSILLMYMVLAAQFESFIDPVTILISL
                     PLSVPFALLSLFLARENFSIIYSSVGILVLFGIVKKNSILQIDHIKKLRAEGLPRLEA
                     IVRGCEDRLRPIIMTTASLVAGMLPLALGTGPGSGSRRTVAIVVIGGQSLALLLTLIV
                     TPVAYSVFDDVARSSVWARIATFLDRGRLSLRRAWAGVTGVVR"
     gene            3045..4625
                     /locus_tag="VFQ79_24180"
     CDS             3045..4625
                     /locus_tag="VFQ79_24180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0142841.1"
                     /translation="MPPRVGVGVTKTRLTLAEATEMALRNNLEIEIEKTNTANAMQAI
                     KTARGAFDPVFRFEPGYENRNTPASSVLQGVGGNLSESFATQNFYFRQKTPWYGSSFH
                     IDFENSRQATSNTFATLNPLRTSRFLAGVSFPLLRNRDTDAERAEIRIRSRQADISDV
                     DFELRVIDVVARVEQAYWDLVAARQDVQVKADGVEWAREQLARNKRMIDAGTLAPVEL
                     AASEAELQRRIDTWLTSVGVVTEVENHLKSLLAPGRDSPLWGDQIVPSDVNLMAPPTD
                     ELQEAVARALKQRPELRQVGLRLDANEVQKELNANQAKPQVNLVANYWSSGLGGSIRP
                     GENPFSASNTVLYQRLNQLSSIAGLAPIDAPAFDDLPQNLVGGYGTAFDNLFAGRYQS
                     VQVGIAFDLNLRNRTAEANLAQSTIAERRLKLERSRMEQLIQAQVRNSLQALETARQR
                     ITAAEASERAAREKLESETRLFQSGESTNFLVLTRQNEYLDSRGRAVVARLEFNRAVS
                     RLEQATGSTLQTHKISVP"
     gene            4690..5472
                     /locus_tag="VFQ79_24185"
     CDS             4690..5472
                     /locus_tag="VFQ79_24185"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU0142842.1"
                     /translation="MKLNAAIAFLSVALLLPTCRLYAQDKSLSRAEIEQIVREYIMRH
                     PEVLIDSVRAHQERERTAAQQKSREAVIAKRRELFEDDTSPVAGKPGAGVAIVQFFDY
                     NCGYCRRVAPTLTKLLEQKQEVHLIFKELPILGPESHIAARAALAAGKQGSYVPFHQA
                     VMGLKSPATMAAIEETGRKLGLDMDRLKADMNSAEVQAILVQNQRLASAIGVESTPSF
                     VIGNEVVPGAMDLPAFQALIAKAAKEKTATPAAPLNPKPANP"
     gene            complement(5481..>5949)
                     /locus_tag="VFQ79_24190"
     CDS             complement(5481..>5949)
                     /locus_tag="VFQ79_24190"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU0142843.1"
                     /translation="ELRRIAAWQMRRERADHTWQPTVLVNELYLELIRIRALKPGQDK
                     AGEKEDFLRLAAHLMKRLLIHHSRPLYRQVEKVPLEGIAESDRTGSEALAEIDAALSR
                     LAAVSPRLRAVVELKVFEGLTGDEIAQRLGCGPATVARDWNFARHWLEEELQP"
BASE COUNT         1193 a         1715 c         1821 g         1220 t
ORIGIN      
        1 tcattcagtt cgttctggaa aaagaccccg aggtggccgc tcaggaagta cgggataagg
       61 tcaacacgat ccttggcgat ttgccgcgtg atgccgaccc cccgattatc gaaaaggtcg
      121 ccaccgatgc cgcgcctgtc atcagcatcg tggtggcgtc cggccgcgat ctgcgcgaga
      181 ccactaaagt ggtggacgac cgcatcaaga agaacatcga aggtttgagc ggtgtcgggc
      241 aggtgcggtt cgtcggagaa cgccagcggc agattcaggt ctggctggat ggcgagaagc
      301 tctatgccta caacctgaat atcgatcaga ttcgtgccgc actggccgct cagaatgtcg
      361 agatccccgg cggcagggtg gaccagggca accgggagtt atcgctccgg actctcggcc
      421 gtgtcgaaca tccgaaggat ttcgaacgga tcatcgtcgg aaacatcggt ggatcgccgg
      481 tccgcatttc ggatatcggc tacgtggtgg atggagtgga agaaccccgc tcccttgcgc
      541 gcctgaacgg gacgtcggcg gtggtgttgg aggttcgcaa gcaggccggg acgaatacgg
      601 ttgaggttat caataacata aaggagcgca tcgccgagct taagagcact ctgccgcccg
      661 atttcgagat cacgtacacg cgtgacactt cgacatttat cgaaggatcg ttccacgccg
      721 tgcaggaaca cctgctgctc ggcggttttc ttgccgcgat catcgtgctg gtgtttatcc
      781 ggagctggcg gtcgacgctg atcgccgcgg tggcgattcc cacctccatc atctcgacct
      841 acagcctgat gagctggatg ggtttttcgc tgaaccagat caccatgctt gcgctgacgc
      901 tgatggtcgg catcgtcatc gacgacgcca tcgtcgtgct cgagaatatc ttccggttca
      961 tggaggagaa gggtctgcct ccggtggaag cagccatcca gggaacccga gacatcggaa
     1021 tggccgtaat ggccaccacc ctcagcctgg tgatcatctt cctgcccgtg gcgctgatgg
     1081 gcggcatcgt cggacgcttc atgtccagct tcggctacac ggcggcgttc gccatcatgg
     1141 tttcgctgct ggtcagcttc acgctgactc ccatgctttg tgcgcgcttc ctgaagccgc
     1201 cggagaaaaa ggagggcgag ggagaggtgc cgggcagctc aacgaaagac acgtgggtct
     1261 tccgttacat ggcgtcggga tactggaagc tgctgcactg gtcgatgaac caccgcaaga
     1321 ttgtcgcttt cgtgtcgttg ctggtggttg tctccaccgt gcctttgttc atggcggtcg
     1381 gcaaggactt tctccctacc gatgaccaga gcgagttcga ggtcacggtg cgcatgcccg
     1441 cggggtcgtc tttggccggg acggaagcgc tgatgcggga actggaagcg gagctcaaga
     1501 cattgccggg cgttcaggat ctgctcacca ccatcggcgc ggacacacgc cggcaggtgg
     1561 atcgcgggtc gatcctggtg gaactcctgc cgatggatga gcgcacccac agccagcagg
     1621 aactgatgtc gatggcgcgg gaaaaattgc ggaagttccg cgagctggta atcgccgtcc
     1681 aattgccgag cctgatccaa ggcgccggtc ccaaccagga tttccagttc ttcattcaag
     1741 ggccggattt ggagcagatc gacaaatacg cccaacaact gaagcagcgg ttggcggagg
     1801 tacctggtgt ggctgacctt gacagctcgt atgaaggcgg caagcctgag ctgcgcgtga
     1861 atatcaatcg cgacaaagcg gcggacctcg acgtcagtgt ctcttccgtc gccaccgcct
     1921 tgcgcactct ggtggggggc gacgagcagg tgaccaccta tcgcgagggc gacgaccgct
     1981 acgacgttca actgcgcgtc cgcgaggagt tccggaattc gccgcgcgcg ctggagcgcc
     2041 tctatgtgcc gtccgggacg ttaggcaatg tccccatctc caatgtggcc agcctgaaag
     2101 aatcgatcgg cccgacgcag atcgagcgtt ataaccggca gcgccagatc atggtttccg
     2161 ccaacgtcgt ggaaggccag tcgctcagca acgtgctcca gattgtagac cggaccgtcg
     2221 cagacatgaa tctgccggcc gagtatcgca ccggtttgct gggtcagagc aaggagttcg
     2281 gacgcgcttt caccaactac gtcatcgcgt ttctgctatc gattctgctg atgtacatgg
     2341 tgttggccgc ccagttcgaa agcttcatcg acccggttac gattctgatc agcctgccgt
     2401 tgtcggtgcc tttcgcgctg ctgtcgctgt ttctcgcgcg ggagaacttc tccatcatct
     2461 acagctcggt tggcatcctg gtgctttttg gaatcgtgaa gaagaactcg attctacaga
     2521 tcgatcacat taaaaagctg cgcgctgagg ggttgccgcg gctggaagcg atcgttcggg
     2581 ggtgcgaaga ccggctgcgg cccatcatca tgaccacggc ttcgctggtg gccggcatgt
     2641 tgccgcttgc gcttggcacg ggaccaggtt ccggatcccg aaggacagtg gccatcgtcg
     2701 taataggcgg ccagagcctt gccttgctat tgacattgat cgttacgccg gttgcgtatt
     2761 cggtctttga cgatgtggcg cggtcgtccg tttgggcgag aatcgcgaca tttctggacc
     2821 gcggccggtt gagtcttcgc cgtgcgtggg ccggcgtcac tggagtggtg cgttaactta
     2881 tgcgtttttt ggttctctcg atatgctttt cctggccgct gttggcgcag acggctgccg
     2941 gaacgtttct tccctcgacg gctgcggcgc cggcctcccc ggccggatcg tccgcggccg
     3001 tccctctttt ttcacaggct ccagctccat cggatgatct ggcaatgccg cccagagtgg
     3061 gggtcggcgt gaccaaaaca cgtctcacgc tcgccgaagc caccgagatg gcactgcgga
     3121 acaacctcga gatcgaaatc gaaaaaacga atacggcaaa cgcgatgcag gccataaaga
     3181 ctgcgcgggg agcattcgat cccgtatttc gtttcgagcc gggctatgag aaccgtaaca
     3241 ctccggcctc ctccgtcttg cagggagttg ggggaaacct ctccgaaagc ttcgccacgc
     3301 agaatttcta tttccggcag aagacgccgt ggtacggttc ttcgttccat atcgactttg
     3361 aaaactcgcg ccaagccacc agcaatacgt tcgctactct gaatccgctg cgtacatcgc
     3421 gcttcctggc aggcgtcagt ttcccgcttt tgaggaacag ggacacggac gcggaacggg
     3481 ccgagatacg cattcggagc cggcaggcgg atatttcgga cgtggatttc gaacttcggg
     3541 tgattgatgt ggttgcccgt gtggagcagg cctactggga cctggtggcg gcccggcagg
     3601 acgttcaggt gaaggccgat ggtgtggagt gggctcgcga gcagttggcg cgcaacaaac
     3661 gcatgatcga cgccggtacc ctcgcgccgg tggaactcgc cgcatccgaa gcggagctac
     3721 agcgccgaat cgatacgtgg ctcacttccg tcggtgtggt gacggaagtc gagaatcatt
     3781 tgaaatcgct gcttgcgcct ggccgcgact caccgctctg gggtgatcag atcgtgccct
     3841 ccgacgtcaa cctgatggca cctccaaccg atgagttgca ggaggcggta gcccgcgctc
     3901 tcaagcaaag gccggagttg cgccaggtag gccttagact cgatgcgaac gaggtacaga
     3961 aagagctgaa tgccaaccag gccaagccgc aagtgaatct ggtagccaat tattggagtt
     4021 ccggcctggg aggctcgatc cgcccgggag agaatccgtt cagcgcatcg aatacagttc
     4081 tgtatcagcg attgaatcag ctttcgagca tcgccggcct tgcgccgatt gatgcccccg
     4141 cattcgacga ccttccgcag aatctcgtcg gcggatatgg aacggccttc gacaacctgt
     4201 tcgccggcag gtatcaatcg gtgcaagtgg gtatagcgtt cgacctaaac ctgcgcaacc
     4261 ggacagccga ggccaatctg gcgcagagta cgattgccga acggcggttg aagctggagc
     4321 gatcccgcat ggaacaactc atccaggcgc aggtgaggaa ttcgctgcag gctttggaaa
     4381 cggccaggca gcgtatcacg gccgcggagg cgagcgaacg tgccgctcgt gaaaagctgg
     4441 agagcgaaac ccggctgttt cagagtggag aatcgacaaa cttcctggtt ctgacacgcc
     4501 agaacgaata tcttgattca cgtggccgcg cggttgttgc gcggcttgaa ttcaaccgcg
     4561 ctgtatcgcg gctggaacag gctacaggga gcacgctcca aacgcataaa ataagcgttc
     4621 cgtagctacc gacggtgccc gtgatagaaa tcattggtgt tttcgccctt gataatgaga
     4681 ggatctgaca tgaaactcaa tgcggcaatc gctttcctga gcgttgcgct actgctgccg
     4741 acttgccggt tgtacgccca ggataaatct ttatcgagag ccgagatcga acagatcgtt
     4801 cgcgaataca tcatgcggca tccggaggtg ctcatcgatt ccgtgcgcgc ccatcaggag
     4861 cgcgaacgga ccgccgcaca gcagaagtcg cgcgaagcgg taatcgcgaa gcgccgcgag
     4921 ttattcgaag atgacacgtc gccggtggcc gggaagcccg gcgctggagt tgccattgtg
     4981 cagttcttcg actataactg cggctactgc cgccgggttg caccgaccct gacgaaactg
     5041 ctcgagcaaa agcaggaagt gcaccttatc ttcaaggaac ttccgattct cgggccggag
     5101 tcgcacatcg cggcgcgggc ggcgctggcg gccggcaagc aaggctccta cgttccgttt
     5161 caccaggccg tgatgggtct caaatcaccg gccacaatgg cggctatcga agaaaccggc
     5221 cgtaagctgg gcctggatat ggaccgtctg aaagccgaca tgaactccgc ggaggtccag
     5281 gcgattctgg tgcaaaacca gcggcttgcc agcgcgatcg gcgtcgagtc gaccccgagc
     5341 tttgtaatcg gaaacgaagt ggtgcccggt gcaatggatc tgcctgcttt tcaggcgctg
     5401 atcgccaaag ccgcaaaaga gaaaaccgct actccagcgg cgcctttgaa ccctaagccc
     5461 gcgaatccgt aggcgcggaa ttatggttgc aactcttctt ccagccagtg ccgggcaaag
     5521 ttccaatccc gcgccacggt cgccggacca catccgagcc tttgcgcaat ctcgtcgccg
     5581 gtgaggcctt cgaaaacttt cagttcgact actgctctga gccgtgggct aaccgctgcc
     5641 aatcgagaga gcgccgcgtc gatctccgcc agtgcttcgc tacccgttct gtccgattcc
     5701 gcaatacctt cgagaggcac tttctcaacc tgccggtaaa gggggcggga gtgatggatc
     5761 agtaagcgct tcatgagatg agccgccagc cggagaaagt cttccttctc cccagctttg
     5821 tcttgcccgg gcttcagagc ccggatcctg atgagttcca gataaagctc gttgacgagc
     5881 acagtgggct gccaggtatg atcggcgcgc tcgcgccgca tctgccacgc ggcgatcctt
     5941 cgaagctcc
//
LOCUS       DASTMK010000588         7413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_318421, whole genome shotgun sequence.
ACCESSION   DASTMK010000588 DASTMK010000000
VERSION     DASTMK010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318421"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFQ79_24195"
     CDS             <1..217
                     /locus_tag="VFQ79_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HEU0142844.1"
                     /translation="RRDYIMKLLSKVSEEDRTLILLKELEGHSVEELAQMMGMNENTI
                     KVKLFRARQKLVKAAQRLSRTPGTALA"
     gene            complement(236..406)
                     /locus_tag="VFQ79_24200"
     CDS             complement(236..406)
                     /locus_tag="VFQ79_24200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142845.1"
                     /translation="MPFELPPQPRIDGPFEVSLETFFSRPMHILPSKILHTTLSVTIA
                     GLTPGYLVEQCS"
     gene            795..1190
                     /locus_tag="VFQ79_24205"
     CDS             795..1190
                     /locus_tag="VFQ79_24205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142846.1"
                     /translation="MFDDTRASWQNPRILSTLLLVFLCGAAVGVLAMRSGAHDTLHRS
                     ADPGEAHESSGRDAMLERFKKELNLRPDQAEQMELVLDDFWKYYQTLQAQMDEVRATG
                     KDRILRILDEDQRKRFDQMVSDLQTRQIR"
     gene            complement(1253..2251)
                     /locus_tag="VFQ79_24210"
     CDS             complement(1253..2251)
                     /locus_tag="VFQ79_24210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142847.1"
                     /translation="MTRRVPAIFAALACTAAIGYAQSVISAHSGLVHYIEGEVFLAEK
                     PVEPKFGKFPEIEEGAVLRTGEGRAEVLLTPGVFLRLGEDSAIKMLSSRLIDTRIELV
                     QGSILIECVELLEGNNIAVAASDRTIEIAKRGLFRLDAQPATLKVYDGEARVGRSGEI
                     DTVKVKDGRELALSDSAAEPAKFDDEDGDALHRWAQRRAGYLAMANVSAANSVRRSSY
                     DWRSSGWLWNPYFGMLTYVPYRSSFYSPFGYQFWSPRQVYQVYEPRVWRAPQQGGGGT
                     YEPRYNSNVGYRTAPRISGSYPSAPSGGSAPAAAPAPSVRTSESAAPRQSSGGRGR"
     gene            complement(2341..4731)
                     /locus_tag="VFQ79_24215"
     CDS             complement(2341..4731)
                     /locus_tag="VFQ79_24215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142848.1"
                     /translation="MRARSRFLPFLFVLCVPLVLPEGLEAQEAITSFRVYTEPPNAPF
                     RVDGRDYVGGATFLWPKGSKHIIEAVETRSPFAGTRYVLKEWRDASNNLVPYSAPVQT
                     VTADPAVPSLQVVYAVHHLIRLRFFDGPVSPQPQCGAPGDAAPGVLRTGVVWIGDACF
                     WTNADIWGEEGDIPINAFPYPGFVFRGWYGASGAYLSSFPLKGQVTLIPRFEPAKRVA
                     FRTSPPGLDVIIDRTQTSTPASEPCTVTQALPPAAPLSVRSLCVGDYDFADGTQHVLS
                     APPFQSHKETGDYWVFEEWDIGGGQNTVYTVKDVNLPVTIRAKFVPGVRIALLTSPSG
                     LKLNIDGRDMAASNFIWGVGSTHRFTAPPEQTDSSGRKYVFKRWSNGGPASQELKVEG
                     AALEMGVRLTAEYELLGRTVLTSSPAGVRLRIDGQECGTPCVLDRPNGTAVSVSAPQS
                     KLLNSDERLEFSGWSDGGGQERTITVENAARTITAHYQSHFRLQILVDPENGATIALS
                     PASADLFYPSGAEVTATVQPNKGYRFRRWEGDAAGTYPIAQIRLNGPVQIAARLEAIY
                     QGPVAEIFNAAGGSPGAAVASGSLISIFGSDLARSSVASEAPPLPQLLGGVIVRIAHR
                     MFPLLFVSPEQINAQLPSDLEPGEYEVVIEREGQEAVKATVLVARNAPGLFSTGTEDR
                     PLAVAYREDGSSVDAAQPARPAELVTILGTGFGPGDRPILDGFAAPEHPPVELVDRVD
                     ILFGDQVIPAEWAGVAPGQVGISMVKFRMPETPEADLPVSVRVNGATSNTVLIPLR"
     gene            complement(4871..7234)
                     /locus_tag="VFQ79_24220"
     CDS             complement(4871..7234)
                     /locus_tag="VFQ79_24220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142849.1"
                     /translation="MRKHALCFLLLGALSAIAAAPAAAGQAGGTEPELRLYPDRPRLV
                     FRPVRDRGFGRSFEEVRKLYRSDRTFRSIFDKALAQELPKQQPAMLAASWVVTGEDRY
                     AEAAVHALLENPISRSGGGGYSNIWSFGLAYDWLYHHPALTPEKREKAAARIIERLET
                     ELAGLDQASMALWHGRNQAANNAMVGALAVGDLPGQALNLRRALKHYAIALHAIDFSE
                     GWPEGASYWIYNRAGPYAVTADCVLTALGLERVDGISIREAMRKIGLWSVYQYAPNGM
                     FEPYGDSAGSLRLGETGWWEVTVDYFAKLSRDPLVMAGGDYFRNQSPSPYGRRPLYWY
                     VALSYDPSARPRSGYDPAKPELWMRRHMPRAKLFGRESMGVAFFRGEWGNPDELYATF
                     KAGDLLAHHDHYDTGHFSIQRGGLLAPQTGLYGPGGGYYGRHRLGYAIQTVSANSLLV
                     LAPGETAAALQNQKDPVWTSLSGGQRVIRPTGFDCVSLDHFKRMLSSGPHLERADITA
                     FESVPGQFDYIAADITAAYNSTRWAEPGNTAKVSLVTRQFLYLRPEEAFVVYDRVETT
                     RPEYLPKFLLHHLSKPRTESERLLSGNNVEDGILETADRELLSSHQRGILSHRVLLPA
                     RARTLKVGGPNYNCYVEEDGDPSDGFDGVNLEGGDPLQPRPTAQLGLWRTEVEPSTPG
                     TSHRFLNVLLPRIESYGDALPAVEVVDTGAGAYAARVGSTVVVFAGEAKPLEEIHLKV
                     KTPGRLILLDAAPDRQYMVRNRRARASREGVLSTGLPAGAVPVVQVR"
BASE COUNT         1518 a         2269 c         2186 g         1440 t
ORIGIN      
        1 acggcgggat tacatcatga agttgttgtc gaaggtgtcg gaagaagata gaacgctgat
       61 tttgttgaag gaattggaag gacattcggt agaagagttg gcccagatga tgggaatgaa
      121 cgaaaatacg atcaaggtca agctcttccg ggcgaggcag aagctggtga aggcggcgca
      181 gcggttatcg cgtacgcctg gcacggcttt ggcctgataa cgggatctaa cgtctttaag
      241 aacactgttc caccagatag ccgggcgtca gacccgcgat ggtgacggac agggtggtgt
      301 gaagaatctt tgagggaaga atatgcatag gcctgctgaa gaaggtctcg agggatacct
      361 cgaagggacc atcgatacga ggctgaggcg ggagttcgaa cggcatcttg acgcttgcga
      421 tcaatgtcgg gctacagtgc gaagcatgag ggatcagcga catctgattt ctcaattgcg
      481 cgcagaggag ccaattgaac ccgctccggg tttctacgca cgtgtaatag agcgtattga
      541 gtcacaacgc tacggatccg tgtggtcgtt atttctggac ccggcgtttg gccgccgcct
      601 cttttatgcg tccgtcacac ttcttgtgtt gctcgggact tacctggttt cgaccgagcc
      661 cgtagggccc gtcggcccaa ggccggagtc catgtttgtc gccgtcgaac gccagtcccc
      721 gccgagtacg gaaatacagc gcgagcggga tacaatcttg gtaaatctgg ccacgtaccc
      781 ggattagtgt atccatgttc gatgacactc gagcttcctg gcaaaatccc cgtatcctgt
      841 ccactctcct cctggtattt ctctgcggcg ctgcggttgg cgtgctggcc atgagatccg
      901 gggcacacga cacgctgcat cggtccgccg atccagggga agcgcacgag tcgtccggac
      961 gggacgccat gctggagcgc ttcaagaaag aattaaatct ccgccctgac caggcggaac
     1021 agatggagct ggtgctggac gatttctgga agtattacca gacattgcaa gctcagatgg
     1081 atgaagtgcg agcgactgga aaagaccgga ttctacgcat tctggatgaa gatcagcgga
     1141 aacggttcga tcagatggtc agcgatctcc agacccgtca gatccgttga acattgcagt
     1201 gaccatccgg aagcccggag gttatccctc cgggcttcct ctataagacc ggttagcggc
     1261 cgcggccgcc ggaagattgc cggggagccg cactctcaga ggtccttacg ctcggcgcag
     1321 gagcggctgc cggagcagag ccgccgctgg gagccgacgg atagctaccc gaaatgcgag
     1381 gagccgtcct gtagccgaca ttggagttgt accgcggctc gtacgtccct ccaccccctt
     1441 gctgaggcgc gcgccatacc cgaggttcgt aaacctgata aacctggcgc gggctccaga
     1501 actgatatcc gaaggggctg tagaaggagc tgcggtaagg cacataagtc agcatgccaa
     1561 agtacggatt ccacagccat ccgctggaac gccagtcata ggacgaccgg cggacggagt
     1621 tggccgcgga aacatttgcc atcgcaaggt atccggcgcg cctttgagcc cacctgtgga
     1681 gagcgtcgcc gtcttcgtcg tcaaacttgg caggttccgc cgcggaatcc gacagagcaa
     1741 gctcgcgtcc atcttttact ttgacggtgt cgatctcgcc ggatctgccg acccgtgcct
     1801 cgccgtcata caccttcagc gtggcaggct gtgcatccaa ccggaacaat ccgcgtttgg
     1861 cgatctcgat ggtgcgatcg ctcgcagcca ccgcgatatt attgccttcc agcaattcca
     1921 cgcattcgat gagaatggaa ccctgaacca gctcaatcct ggtatcgatt aagcggctgg
     1981 aaagcatctt gattgcgctg tcttcgccga ggcggagaaa aactcccgga gtgagaagca
     2041 cttcggctct accctcgccg gtgcgcaata cagcgccttc ttcgatctcc ggaaactttc
     2101 caaacttcgg ctccacgggt ttttccgcca ggaacacttc cccttcaatg tagtggacca
     2161 acccggaatg agccgagatt acggattggg cgtaaccgat cgctgccgta caggccagtg
     2221 ctgcaaagat tgctggcact ctgcgggtca tcacgcctcc ttgatagcta tcctgtccct
     2281 attagggacg cctgtcaacc gcgtttcgtt gcaaaccaga caccaccagt gtaaaacaag
     2341 ctatctcagg gggatcagga ccgtattgct cgtcgcgccg ttgacccgga cggaaaccgg
     2401 caggtcggcc tccggcgtct ccggcatccg gaatttgacc atactaatcc caacctgtcc
     2461 cggggcaact cccgcccact cagccggaat aacctgatct ccgaagagaa tatccactcg
     2521 atccacgagt tcgaccggcg ggtgctctgg tgcggcaaaa ccatcgagaa tcgggcggtc
     2581 tcccggtccg aatccggtac cgaggattgt caccaactca gcgggacggg ccggttgggc
     2641 ggcatcgacg gaggagccgt cttcccggta ggctaccgcc agcggccggt cctcggtgcc
     2701 tgtcgagaac aagcctggag cgttccgggc gaccaatacc gtagccttca cagcttcctg
     2761 tccctcgcgc tcgatcacaa cctcgtactc gccgggttcg agatcggatg gaagctgtgc
     2821 gttgatctgt tcaggcgaca cgaacaggag tggaaacatc cggtgagcga tccgcacgat
     2881 caccccaccc aatagttgcg gcaaaggagg cgcttcgctc gctacggaag aacgcgccag
     2941 atctgagccg aagatcgaga tgagagatcc ggacgctacg gcggcgccgg ggcttcctcc
     3001 ggcagcgttg aaaatctccg caacaggccc ctggtagatg gcttcaagac gcgccgcgat
     3061 ctggacgggg ccgttcaacc tgatctgtgc aatcgggtaa gtgccagccg catcgccttc
     3121 ccaacgccgg aaccggtatc ctttattcgg ctgaaccgtt gcggtgactt cggcccccga
     3181 tgggtagaac agatctgccg aagcggggct gagggcaatt gtcgcgccgt tttcgggatc
     3241 taccaatatc tgcagccgga aatggctttg gtaatgagcc gtgatggtgc gggcggcgtt
     3301 ctcgacggtg atggtccgtt cctgccctcc gccgtccgac cagccggaaa actccagacg
     3361 ctcgtccgaa ttcaggagtt tagattgtgg cgccgaaact gaaaccgctg taccgtttgg
     3421 ccggtcgagc acacaggggg tgccgcactc ctgcccgtcg atgcgtagcc gtactccggc
     3481 tggagagctc gtcaacacag tcctgccaag cagttcgtat tccgccgtca ggcgtacacc
     3541 catttcgagc gccgcccctt ccactttcaa ctcctgcgac gccggaccac cgttcgacca
     3601 ccgtttaaaa acgtatttcc gtcccgagga atccgtctgc tccgggggtg ccgtaaacct
     3661 gtgggtggaa cccacacccc agatgaagtt cgatgcagcc atgtcgcgcc cgtcgatgtt
     3721 cagtttcagg cccgaagggc tggtgagcag cgcaatacgg acaccgggaa cgaattttgc
     3781 ccggatggtg actggcaggt tgacgtcttt caccgtgtag acggtattct gtccaccgcc
     3841 gatgtcccat tcttcgaaca cccagtaatc accggtctcc ttgtgactct ggaagggcgg
     3901 cgccgaaaga acatgctgtg tcccgtccgc aaaatcgtag tcgccgacac acagcgaccg
     3961 gacactcaac ggagctgccg gaggcagcgc ctgcgtgacg gtgcaaggct cggaggcggg
     4021 cgtactggtt tgcgtccggt cgatgatgac atcgagaccg ggaggcgaag tgcgaaacgc
     4081 cacgcgttta gccggttcaa aacggggaat taaggtaact tgtcccttca gcgggaagct
     4141 gctcaggtag gcgccgctgg cgccgtacca gccccgaaag acaaagcccg ggtagggaaa
     4201 ggcgtttatg ggaatgtccc cttcctcacc ccagatgtcg gcattggtcc agaaacaggc
     4261 gtcgccaatc cacacgacac ccgttctcaa tacacccggc gccgcgtcgc cgggagctcc
     4321 gcattggggt tggggggaaa ccggtccgtc gaagaaacgg agacggatca ggtggtgaac
     4381 ggcatacact acctggaggg aaggaacggc cgggtcggct gtcaccgtct gcactggagc
     4441 gctatatggc accaggttgt tcgaggcgtc cctccattct ttaagaacat accgggttcc
     4501 ggcgaaaggg cttctggtct cgaccgcttc tattatgtgc ttgctgccct tgggccagag
     4561 aaacgtcgct ccgcccacat aatcccgccc gtccacacgg aaaggcgcgt taggcggctc
     4621 cgtatacacc cggaagctgg taatcgcctc ctgcgcctcc aggccctcgg ggaggaccag
     4681 cggcacacat agaacaaaca ggaacggcag aaaccggctt cgtgccctca ttcgctctcc
     4741 ttcttgagac aagggccaag acgaggcccg cacgactaac taatcggcgg tcccgttcat
     4801 cagctttagg cggcgttgcg aatggcggct ttggcgttgg aatgccggta attcagtttg
     4861 ggttgaagct ctatctcact tgaacaaccg gtacggctcc agccggcaga ccggtcgaga
     4921 gaaccccttc ccggctggcg cgcgcccgtc gatttctcac catgtactgg cggtccggcg
     4981 cagcatccag aaggatcaga cggcccggcg tctttacttt caagtggatt tcttcgagtg
     5041 gtttcgcttc gcctgcgaat accaccacgg tacttcccac ccgcgcggcg taagcgcccg
     5101 cgccggtatc cacaacttct accgccggta aagcgtcgcc gtaggactcg atccgtggca
     5161 gcaggacgtt caggaaacga tggctggttc ccggcgtcga aggttccact tccgtccgcc
     5221 agagtcccag ttgtgcggtg ggccgcggtt ggagagggtc gcccccttcc aggttcacgc
     5281 catcgaaccc atcggacgga tcgccgtctt cttcgacgta gcagttgtag ttcggcccgc
     5341 cgaccttcag cgtacgcgct cgagcgggca acaacacgcg gtgactcaaa atgccgcgct
     5401 gatgcgaaga cagcagttcg cggtcggccg tttccaatat gccgtcttcg acgttgtttc
     5461 ccgacagcag cctttccgat tccgtccgcg gtttggagag atggtgcaac agaaacttcg
     5521 gcagatactc gggcctcgtc gtctccacgc gatcgtagac cacgaaagcc tcctccggac
     5581 gaagataaag gaactgccgc gttaccaggg aaaccttggc cgtgttgccc ggctccgccc
     5641 atcgggtcga gttgtatgcc gccgtgatat cggcggcaat gtagtcgaac tgtcctggta
     5701 cactctcaaa cgcggtgata tccgcacgct cgagatgcgg ccccgaactc aacattcgct
     5761 tgaaatggtc cagactgaca cagtcgaatc ccgtcggccg gatcacccgc tggccgccgg
     5821 agagcgatgt ccacacgggg tctttctggt tttggagtgc cgcggcggtc tcgccgggag
     5881 cgaggaccag caggctattg gctgaaacgg tctggatcgc gtagcccagc cggtgccgcc
     5941 cgtaataccc gccacccgga ccatacaggc cggtttgcgg cgcgagcagg ccgccgcgct
     6001 gaatgctgaa gtgcccggta tcgtagtgat cgtggtgtgc gagcaggtcg cctgccttga
     6061 acgtggcata gagttcatcc ggattacccc actctccgcg aaagaacgcc acacccatcg
     6121 actcgcggcc gaacaacttc gcccgcggca tgtgcctgcg catccacagc tcgggctttg
     6181 ccggatcata gccgctcctg ggccgcgccg aagggtcata agacaacgcg acgtaccaat
     6241 acagcggcct ccttccatac ggcgacggag actggttgcg gaaataatcg ccgccggcca
     6301 tcacaagagg atcgcgcgat agtttggcaa agtagtccac cgtcacttcc caccagccgg
     6361 tttcgcccag gcgcaatgag cccgcggaat cgccgtacgg ctcaaacatc ccgttcggag
     6421 catactggta caccgaccac agcccgatct tccgcattgc ctcgcgaatc gaaatcccat
     6481 cgacccgctc caggccgagc gccgttaaaa cgcaatcggc ggtcaccgca tagggcccgg
     6541 cgcgattgta aatccagtag ctcgctcctt ccggccagcc ctccgaaaag tcgatggcgt
     6601 gcagagcgat tgcatagtgt ttcagcgcgc gccggaggtt caaagcctgc ccgggaagat
     6661 cgccgaccgc gagcgctccg accatggcgt tattggccgc ctggttgcgg ccgtgccaaa
     6721 gcgccatcga ggcttgatcg agtccggcca gttccgtctc gagccgttcg atgatgcgcg
     6781 cggccgcctt ttcgcgcttc tccggagtga gtgcggggtg atggtacaac cagtcatagg
     6841 caagcccgaa agaccagatg ttggaataac cacctccgcc gctgcggctg atcgggttct
     6901 cgagcaacgc gtgaacggct gcctcggcat accggtcttc gcctgtcacc acccacgatg
     6961 cagccagcat cgccggctgt tgtttcggca actcctgcgc cagcgcctta tcgaaaatcg
     7021 aacggaaggt gcgatccgag cgatacaatt tccgcacttc ttcgaaactg cggccgaaac
     7081 cccggtcgcg aacggggcga aacaccagcc gtggacgatc cggatagagc cgcaactcag
     7141 gctctgtgcc gccagcctgt cccgcagccg ccggcgcggc ggcgattgcg gacagcgcgc
     7201 caagcagcaa aaagcacagg gcatgtttac gcatgtggtc ctatggggga atcaggacaa
     7261 gcggccgccg tcacgccaca tcctctacgc cgactacaat ccgctttccg aggacctcga
     7321 gcgcagcctg caatttcttg gttttcgtgt cgtggtccgg gtctaacatg cgccgcacaa
     7381 ccgtttcacc cacgcccagg cgccgggcca act
//
LOCUS       DASTMK010000589        13272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_638508, whole genome shotgun sequence.
ACCESSION   DASTMK010000589 DASTMK010000000
VERSION     DASTMK010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638508"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFQ79_24225"
     CDS             <1..384
                     /locus_tag="VFQ79_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0142850.1"
                     /translation="GYFFRAVAHIHPRHRTPSVSILLLSVWASVLVLSGRYEQLFTYV
                     IFASWILYAMAAAAVIVLRKKRPELERPYRTIGYPVVPVLFVTGAAFVLISTLRNSPR
                     ESLMGLALIAAGIPFYFVWKRKHLV"
     gene            587..808
                     /locus_tag="VFQ79_24230"
     CDS             587..808
                     /locus_tag="VFQ79_24230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142851.1"
                     /translation="MDVTKILAELRQEREQLEEAILSLERLARGRGKRRGRPPAWMST
                     VKRRGRPPGSKNKREPNSKNTTPTKAAVA"
     gene            complement(822..3203)
                     /locus_tag="VFQ79_24235"
     CDS             complement(822..3203)
                     /locus_tag="VFQ79_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEU0142852.1"
                     /translation="MKRSRSHSTAARFTGLPLHLLAIVLSTASLAHAAQGTAFESGLA
                     LDAVIEQAIAEDQIPGAVVWIGHKGKVIYRKAYGSRALKPRRERMTLDTIFDAASLTK
                     VVATTASVMKLFEDGKIRMNDPVTKYLPEFQGGNSSITVRHLLTHFSGLRPDVDLEPR
                     WAGYETGVKLAMKDIPVAPPGTRFIYSDINFILLGEIVHRVSGQTLPEFARRHIFLPL
                     GMKTTMFQPPARLRPRIAPTELVDRAILRGVVHDPTTRYMGGVAGHAGLFTTAEDLSR
                     FAEMILGGGERNNVRIFNPLVVRKFSTPQSPADQPILRGFGFDIDSPFSGNRGELFPI
                     GSYGHTGFTGTSLWMDPVSDTYVILLANSVHPRRRPAITSLRARVATVAAAGLGIDLP
                     DVALTGFNETIVGAGMRRTFARNGKTETGLDVLIQEKFALLAGARVGLITNHTGLSLA
                     GQRNIDAMVAAGVNVVAIFSPEHGIAGKEDHENVKNSTDSATGIPIWSLYSGPNRRPS
                     EKMLANVDVLVFDIQDIGARFYTYICTMVNAMEEAAKHGLPFYVLDRPNPITGVHVEG
                     PMLDPDLKSFVGCIVLPLRHGMTAGEIANMVNTEQKLGVKLHVVTMKDWQRGDWFDAT
                     GLTWVDPSPNMRSLNAALLYPGVAMLESLRNYSVGRGTDAPFEQIGAEWIRGRELAQY
                     LNSRYIPGVRVYPARFQPSASNYAGQNIEGVRFVITDRTAFDSYRLGLEIAAALEKLF
                     PGNIAWERTERLIGNRATISALRSGADPRSIIESEGEALRSFVERREKFLIYR"
     gene            complement(3200..3499)
                     /locus_tag="VFQ79_24240"
     CDS             complement(3200..3499)
                     /locus_tag="VFQ79_24240"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU0142853.1"
                     /translation="MPRLPKLNDVQLQSALAGLSGWTIRDGKLHREYLFPDFVHAFGF
                     MATAAIAIEAMNHHPEWCNVYNRVTVELITHDSGGITENDLTLARKLDDLARKLQ"
     gene            complement(3536..4228)
                     /locus_tag="VFQ79_24245"
     CDS             complement(3536..4228)
                     /locus_tag="VFQ79_24245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0142854.1"
                     /translation="MPEFENHVVVISGATGGAGQPITARFLEEGATVFAIGRSRNNKI
                     APARLVRVEADLFTRQGARSVVETAISRAGRIDVLVHLVGGFAGGQSVAEAGAATWEH
                     MMSINFHAALFLFEAVIPEMMKRRHGRIIAMGSRAGVDPVATLGPYSVSKAALHALVR
                     AAAVEVKNSGITSNAVLPGVIDTAANRKANPAADYTAWVKPESIAELILWLASDRAGD
                     VNGALIPIYGRS"
     gene            4290..5552
                     /gene="amrB"
                     /locus_tag="VFQ79_24250"
     CDS             4290..5552
                     /gene="amrB"
                     /locus_tag="VFQ79_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEU0142855.1"
                     /translation="MPTLPALRMDLDFMPSPLADRPGLLIRDPFRYTDTTLIVPPVLV
                     RCLHLYDGQHSDLDVREELVRLTGELAVSEVVSQLTEALSGAGFLHDERGEQLRELRH
                     REFVEAEFRQATHAGSAYPDEHEALRKTMHRYMGAEPESASTDSLVAIAAPHVSPEGG
                     WRSYQAAYRALAPAYRDRTFVILGTSHYGEPDRFGLTRKSFSTPMGTAITDTRLVDRL
                     AASAPNAVIMEDYCHSFEHSIEFQIIFLQHLFGADIRVVPILCGSFGKSIGARQLPEK
                     DDGVRRFLDALGEMGSVEDGRLLWVLGVDMAHIGPRYGDEFSATTGEGKMLEVEQRDA
                     QRIQQVNTGNAEGFWELVCENEDDLKWCGSAPIYTFLRAVPDARGELLHYDQWNIDEQ
                     SVVTFAGMSFRRAEPLPHGHGSETEPRA"
     gene            complement(5567..6571)
                     /locus_tag="VFQ79_24255"
     CDS             complement(5567..6571)
                     /locus_tag="VFQ79_24255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0142856.1"
                     /translation="MPERTFTATVNVVVVPVTITDKQGNYVAGLKPEDFRLYDNNKLQ
                     DIRVEEAVQPISLVVAVQANPDMEGILPMVKQIGPVISQLIIGQNGEAAILAYDGKIR
                     TMQDFTADSDQISSALQKINPGSSMNRVKDAAREAVRMLRDRPPGNRRVLLIIGETRD
                     KSSEGNLREIMAEVQLHNVSVYTANVNRLVSTLTGRPETPRPSPFPATAYKIPGGGPQ
                     TPGTVASLGHGGTLGNWVPVFVEIFRDVKSIFVSNPAEVFTKYSGGKEFSFMKQQDLE
                     RALSEIGSELHSQYMISYMPDNKSEGGFHELRVNVSRPQAADWDIRYRPGYWMAAVPQ
                     "
     gene            complement(6666..7160)
                     /locus_tag="VFQ79_24260"
     CDS             complement(6666..7160)
                     /locus_tag="VFQ79_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU0142857.1"
                     /translation="MIQAAVLTISDSVHRGSRLDLSGPAVRERLEQLGWSIAATEVLP
                     DEADAIAGRLATLADGNQLSAIFTTGGTGIAPRDVTPEATRRIADREIPGLGELMRFE
                     GRKSTPLAALSRSFAGTRGHVLIVNLPGSPQGAVESLNAIVDLVPHILELLRGHTDHS
                     GIRN"
     gene            complement(7192..8046)
                     /locus_tag="VFQ79_24265"
     CDS             complement(7192..8046)
                     /locus_tag="VFQ79_24265"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0142858.1"
                     /translation="MSLCCAVLLGLAFIVAGTWKITDPLSAAARMREALLPAWFSLPA
                     ALSLGISETFAGVLLLVPRYRRWGACLSGLLLIAFLGYFAINYNALRGEECNCFPWIK
                     RTVGPAFIIGDLVMLAMAWFAGVWARPSESKRGAAIILTAVCVFAGVSYGMTVARESG
                     LRAPSTIQVAGQPVALDDGRFFLYFFDPECSHCVFAAKEMSGYRWRDTRIIAIATAMP
                     QFADQFLASTGLKADLSTDIEKLRETFRFASTPYGVALEDGRQKAAFPHFEGAEPERS
                     LRELGFIE"
     gene            8114..8680
                     /locus_tag="VFQ79_24270"
     CDS             8114..8680
                     /locus_tag="VFQ79_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142859.1"
                     /translation="MLLILALLTALVRPGGRLEAELPRVTIIDDTVEIAASKVIGFPL
                     LLRQSAAVVECSYAVDEGGSGVRVLLLRAADAEELYSGHAHRPLISTPYQRSGGFRYR
                     VPLAGDYVLVLDNRMEGRGVSKVRLHLSAVFGQAARETVRYLPRSRRIAVVVISLLVF
                     ASIVTYSGRKLAAAMAARRSGGPPALYG"
     gene            complement(8634..9926)
                     /gene="hemL"
                     /locus_tag="VFQ79_24275"
     CDS             complement(8634..9926)
                     /gene="hemL"
                     /locus_tag="VFQ79_24275"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU0142860.1"
                     /translation="MPHSRNTTPLRLDRSDVLLERARNVIPGGVNSPVRAYKSVGGTP
                     PFILRGEGAKVFDVDGNEYVDYVGSWGPLILGHRHPAIIAGVEKALEHGTSFGAPTEL
                     EVELAEEICAAVPSIEMVRLVNSGTEATMSALRLARGFTGRDVTVKFEGCYHGHVDSL
                     LVKAGSGVATFGIPDTAGVPKAFSDTTIAVPYNSIAAVESVFSQRGGEIAAVIVEPVA
                     GNMGCVPPVPGFLESLRDLTSRHGALLIFDEVMTGFRVAFGGAQQLYGIRPDLTTLGK
                     IIGGGLPIGAYGGRKDIMSQVSPLGPVYQAGTLSGNPLGVAAGLAMLRHLRENPWIYE
                     QLEARTAELVEGAPAGVTVNRIGSMFTFFFTDRPVTDYDSAKRSDTAQFARFFHWMLE
                     NGIYLAPSQFEAGFVSAAHSADDIIRTKQAARRFFEQP"
     gene            complement(9933..11114)
                     /locus_tag="VFQ79_24280"
     CDS             complement(9933..11114)
                     /locus_tag="VFQ79_24280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0142861.1"
                     /translation="MKRIAVLGGGPAGAFAAERLAASGLETVLLDEKLAWEKPCGGGL
                     TYKAYREYPFLIDNDKPKKIVWDTYLSEPKAGSVKMKLTHPLVIYSRFDLNRMLLERA
                     ERAGAQLEKTRVLEMARGASRWRIRTSGGVIDADFCIVATGARNSLRQVGTQWTRNDT
                     MFALGYYVPSMQEHIDIQFLPKLEGYIWVFPRCGHLSVGICGKGESAQSLRKRLEDYM
                     RLRCISWQNAAFYSHVLPSLATPAWKTNHIAGDGWLAVGDAAGLVDPITGEGLYYAMR
                     SGDLASRILVEERHPGSAMTAAYRTVLHRDFAADLAFSATFARRVFVGRFLFGTVPAR
                     MIQFMRRSPRFHDLMQDLFAGTQPYSDLKGRLLRNLNGTLHEVVMNFFFGRLVTGENK
                     I"
     gene            complement(11111..11542)
                     /locus_tag="VFQ79_24285"
     CDS             complement(11111..11542)
                     /locus_tag="VFQ79_24285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142862.1"
                     /translation="MKVYLVVLAAAFLLLGGCRRDIQNTEAIRRSVIDYLSSRSGLDV
                     SSMQVEVTSVTFRENEADATVSFRAKGSNDPAAGMQMAYTLERQGDKWVVKGRRGAGS
                     GSDPHGGIGSDMPPAGGSGDLPAGHPPVPPQSPNEQAQPKK"
     gene            complement(11584..12261)
                     /locus_tag="VFQ79_24290"
     CDS             complement(11584..12261)
                     /locus_tag="VFQ79_24290"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU0142863.1"
                     /translation="MAHPTTEARFAATPEKGEVSSLLIRPENATHLLVLGHGASTNMR
                     HRILQSIADSLAGENIATFRYNFPYMENGRGRDSQDTCTATVRSAVAAAHRAAPGLPL
                     LAGGHSFGGRMTSTAASGQPLDGVRGIVFFAFPLHQPGKPDTKRADHLGAVQIPMLFL
                     TGTRDALAEPDLLRTVCNKIGDRATLHPLDTADHGFRPLKRARESKEDVFLEMARTVR
                     EWVSTLK"
     gene            complement(12289..12975)
                     /locus_tag="VFQ79_24295"
     CDS             complement(12289..12975)
                     /locus_tag="VFQ79_24295"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEU0142864.1"
                     /translation="MKVLTGTALAALLISTGVSGASVPSTQIQGEYIEARTADVYTGP
                     CFANGEVGLVGDLAVMGWRIEKGSWNGVNLDGLSVVGVLKASHTLGDGFQSAYPVKSV
                     LIVDERAGAAQRNALKEFARGMSGDLLQEIVRIEYQPISFAVKDGNVHTATATLKAGN
                     LASIETRALKNGDQICHNEEVWYTPLTKVDHAMPAFTLAHSFNGEGLGTTWSNPGKRS
                     AFVADFHFEK"
BASE COUNT         2755 a         3924 c         3860 g         2733 t
ORIGIN      
        1 ggatacttct tccgcgcggt ggcccacatc cacccgcggc accgcactcc ctccgtgtcg
       61 attcttcttc tgagcgtttg ggcgtcggtt ctcgtcctta gcgggcggta cgagcagtta
      121 ttcacatacg taatattcgc aagttggatt ctgtatgcga tggctgccgc cgcggtaatt
      181 gtactgcgaa aaaaacgccc ggaactcgag cggccctacc ggacgatcgg ataccccgtt
      241 gtaccggtac tcttcgttac gggtgcggca ttcgttttga tctctacgct gcgcaattca
      301 ccgcgtgaat cgctgatggg actcgcgcta attgcggcag gaatcccttt ctatttcgtt
      361 tggaagcgaa agcatttggt ataatcggca cagaatttta tacctccccc gtgacgacct
      421 cctagtactt cggcgatgtg ccttgttctg aaaacagaac aggggtattg aattcacgtg
      481 aaaaataggg taaaacagca tttggcattg tcgcatgcca actcaccttt tgttccggat
      541 tcggaacgga caggcggcgg gtgaaagatt tcctggaggc acatctatgg acgtcacgaa
      601 aatcctggct gagttgcgtc aggaaaggga gcaattagag gaagcgatac tcagcctgga
      661 acggctggcg cgcgggcggg gtaagcggcg gggaaggcct cccgcctgga tgagcaccgt
      721 gaaacggcgc ggacgtccac cgggaagcaa gaataagcgg gagccgaact caaaaaacac
      781 aactcccacc aaggctgccg tcgcctgata tatcttttaa ttcagcgata gatcaggaac
      841 ttctccctcc gctcgacaaa agaacggagg gcctctccct cgctttcgat gatgctgcgg
      901 ggatcggcgc cggaacggag agccgaaatc gtggcccggt tccctatcag acgttctgtc
      961 cgttcccacg caatgttccc cggaaagagc ttttccaaag ccgccgcgat ctccaatccc
     1021 aatcgatagc tatcaaaggc ggtgcggtcc gtgataacga accgcacacc ttcgatattc
     1081 tgtcccgcgt agttcgaggc tgacggttgg aatcgcgccg gatatacgcg aactccaggg
     1141 atgtatcgcg aattcaggta ctgagccagt tcgcggcccc gaatccattc cgcaccaatc
     1201 tgctcgaatg gagcgtccgt gccgcgcccg accgaatagt ttctcaggct ctccagcatg
     1261 gcgactccgg gataaagcag tgccgcattc aggctgcgca tgttcggtga aggatccacc
     1321 caggtcagac cggtggcatc gaaccagtca cctctttgcc agtctttcat ggtcacaaca
     1381 tgcaatttga ccccgagttt ctgttccgta ttcaccatgt ttgcgatttc accggccgtc
     1441 ataccgtgcc gcaacggtaa aacgatgcag ccgacgaacg atttaaggtc gggatcgagc
     1501 atcggccctt ccacgtgcac cccggtgatc gggttggggc gatcgagcac ataaaacgga
     1561 agcccgtgct tcgcggcttc ctccatagca ttcaccattg tgcaaatgta ggtgtagaat
     1621 cgcgcgccga tatcctgtat atcgaacacc aatacatcga cgtttgccag cattttctcc
     1681 gacgggcgcc ggttcggacc ggaatacaag ctccagatcg gaatgccggt ggcgctatcg
     1741 gtggagtttt tgacattttc gtggtcctcc ttaccggcga taccatgctc cggtgaaaag
     1801 attgccacga cgtttacacc ggcggccacc attgcgtcga tattgcgctg tccggcgagg
     1861 ctcaagcccg tgtggttggt aatcaagccg acgcgtgccc cggcgagcag cgcaaacttt
     1921 tcctgaatca gcacgtcgag tccggtctcc gttttcccat tgcgcgcaaa cgtgcggcgc
     1981 atgccggccc cgacaatcgt ttcattgaaa cccgtcagcg ccacgtccgg gagatcgatg
     2041 cctaacccgg ccgcggccac cgtggcgacg cgtgcgcgta aagacgtaat ggcggggcgc
     2101 cgccggggat gcacgctgtt ggcgagcaga attacgtagg tatcgctcac gggatccatc
     2161 cagagggagg ttcccgtaaa cccggtgtgg ccgtaggagc cgatgggaaa caattccccg
     2221 cggtttccag aaaaaggtga atcgatatcg aacccaaatc ctcgcaggat cggctggtcc
     2281 gcgggcgatt gcggtgtcga aaattttctg acgaccagcg gattgaagat tcgcacgttg
     2341 ttgcgctcgc cgccgccgag aatcatctcc gcgaatctcg acaggtcttc ggcggtggta
     2401 aacaggcccg catggcccgc cacgccgccc atatatcttg tggtcgggtc atgaaccacg
     2461 cctcttaaaa tcgctctgtc gaccaattcg gtaggggcta ttctcggccg cagtcttgcc
     2521 ggaggctgga acattgtggt cttcatcccc agcggcagaa agatgtgccg gcgcgcaaac
     2581 tccggcaacg tttgtccgct gacccgatgc acgatttctc ccagcaggat gaaattgatg
     2641 tcgctataga tgaagcgcgt ccccggaggc gcgacaggaa tgtccttcat ggcgagcttt
     2701 acacccgtct cgtaaccggc ccagcgcggc tcgagatcca catccggccg gagcccggaa
     2761 aaatgggtta gcaggtgccg gacggtaatg gaactattac cgccctggaa ctcgggcaaa
     2821 tatttcgtca ccggatcgtt catccggatc ttgccgtcct cgaacaactt catcacggat
     2881 gccgtggtcg cgacgacctt cgtcagggaa gcggcatcga aaattgtatc cagggtcatc
     2941 cgctcgcggc gtggcttcaa cgccctggat ccgtacgcct tccgatagat gacctttccc
     3001 ttatggccga tccaaaccac cgcgccgggg atctggtctt cggcaatggc ctgttcgatt
     3061 acggcgtcca gggccagacc gctctcaaaa gctgtacctt gcgcggcgtg cgccaggctg
     3121 gcggtggaaa gaacaattgc gagtaaatgc agcggcagac cggtaaaccg cgccgcggtt
     3181 gaatgcgaac gggatctctt cattgcagtt tgcgggctaa atcgtcgagc tttcgagcca
     3241 gtgtcagatc gttttccgtt atgccgccgg aatcgtgcgt aatcaactcc acggtgacgc
     3301 gattatagac gttacaccac tcggggtgat ggttcatcgc ttcgatcgcg atggccgcgg
     3361 tagccataaa accaaaggcg tggacgaaat cggggaaaag atactcgcgg tgcaacttgc
     3421 catcccgaat ggtccacccg gaaagtccgg ctagcgcgga ttgaagttgt acgtcattga
     3481 gcttcggcag cctgggcata tttttatcct actaatcgtg tttcgattcg tcttgtcagg
     3541 aacggccgta gattggaatc aacgcgccat tgacatcacc tgcccggtcc gaggcgagcc
     3601 acagaatcaa ttccgcaatc gattccggtt ttacccacgc ggtatagtcc gcagccggat
     3661 tcgccttccg gtttgccgcc gtatcgatga cccccggcag cacggcgttc gaagtgatcc
     3721 cggagttctt gacttcgacg gcggcggcgc ggacgagagc atgcagggca gccttcgaca
     3781 ccgagtaagg tccgagggtg gcaaccggat cgacgccggc gcgggagccc atcgcaatga
     3841 tccgaccgtg acggcgtttc atcatctccg gaatcacggc ctcaaacaaa aacagggccg
     3901 cgtggaagtt gatgctcatc atgtgctccc aggttgcagc tccggcctcc gcaacggatt
     3961 gcccaccagc gaagccgccc accagatgta ccaggacgtc tatccttccc gctctcgata
     4021 tcgctgtttc cacgaccgac cgggcgcctt ggcgggtgaa cagatcggct tcgacgcgga
     4081 cgaggcgtgc aggcgcgatc ttgttatttc gtgagcgccc tatagcgaaa acggtagcgc
     4141 cttcctcaag gaatcgagcc gttatcggct ggcccgcgcc tccggtcgct ccggagatga
     4201 cgacaacatg attctcgaat tcaggcatag tgccagtgta agccgcaacc ggcgccgatg
     4261 gctggcacac tgtttcgtat catgaatgga tgcccacact ccccgcgttg agaatggacc
     4321 tggacttcat gccgtcaccg ctggcggacc ggccgggatt gctgatacgc gatccgttcc
     4381 gttacacgga tacgacgctc atcgttcctc cggtgctggt gagatgttta cacctctacg
     4441 atgggcagca tagcgatctc gatgtaagag aagaactggt acggctcacg ggcgaacttg
     4501 cagtgagcga agtggtaagt cagttgaccg aggccttgag cggtgcgggt tttctacatg
     4561 acgagcgggg cgagcagttg agagagttac gccaccgcga gttcgtcgaa gcggagttcc
     4621 ggcaggcaac acacgcaggt tcggcatacc ccgatgagca tgaagctctc cgcaagacca
     4681 tgcaccgcta catgggggcg gaaccagaga gtgcgtccac ggattcgctg gttgcgatcg
     4741 cggcgccaca cgtcagtccg gagggaggat ggcgatctta ccaggctgcc tatcgcgcgc
     4801 ttgctccggc ctatcgcgac cgcacctttg tcattcttgg cacatcgcat tacggcgagc
     4861 cggaccggtt cgggttgacg cgcaagtcct tttccacacc gatgggaact gccatcacgg
     4921 acacgcgtct ggtggaccgt ctggcggcaa gcgcgcccaa cgccgtcatc atggaggatt
     4981 actgccactc gttcgaacac tccatcgaat ttcagataat ctttctccag caccttttcg
     5041 gtgcggatat cagggtagtc ccgatccttt gcggatcctt cggaaagagc ataggcgccc
     5101 ggcaactgcc cgaaaaagac gacggcgtga ggcgcttttt ggacgcgctc ggcgagatgg
     5161 gaagtgtcga agacggccgg ctgctctggg tattgggtgt cgatatggcg catatcggtc
     5221 cgagatacgg agacgaattt tccgctacca ccggggaggg aaagatgctc gaggtggagc
     5281 agcgggatgc tcaacgcatc cagcaagtga acacaggcaa cgcagagggt ttttgggaac
     5341 tagtttgtga aaacgaagac gatctcaagt ggtgtggatc ggctccaatc tacacgtttc
     5401 tccgcgctgt accggatgcg cgaggcgagc tccttcacta cgatcagtgg aacatcgacg
     5461 agcaaagcgt agtgacgttt gccggaatgt cgttccggcg cgcggaaccg ctccctcacg
     5521 gtcacggctc ggaaacggag ccgcgcgcgt aagcgagcgg gagcggctat tgtggtacgg
     5581 ctgccatcca gtagccgggc cggtagcgga tgtcccagtc ggcggcctgc gggcggctca
     5641 cgtttaccct gagctcgtgg aagccgcctt ccgatttgtt atcgggcatg tagctgatca
     5701 tgtactggct gtgtagttca ctgccgattt cagagagcgc ccgttccagg tcctgctgct
     5761 tcatgaaaga gaactctttg ccgccgctgt atttcgtgaa tacttcggcc gggtttgaaa
     5821 cgaagatcga cttcacgtcc cggaagattt caacgaacac cggaacccaa tttcccagcg
     5881 tgccgccatg gcccagcgac gcaacggtcc ccggcgtctg tggaccgccg cctggaattt
     5941 tgtaagcggt agccggaaag ggactcggcc gcggcgtttc gggcctgccg gtaagagtgc
     6001 tcaccaggcg attcacattg gccgtgtaaa cgctgacgtt gtgtagctgc acctcagcca
     6061 tgatttcgcg gaggttgcct tcactcgatt tgtcccgcgt ctcgccaata atcagcaaca
     6121 cccggcggtt gccggggggc cggtcgcgaa gcatgcgaac ggcttcgcgg gccgcatcct
     6181 tgacgcgatt catgctgctg ccggggttga ttttttgtaa ggcggaagag atctggtccg
     6241 aatcggcggt aaaatcctgc atggtccgga ttttgccgtc gtaagccaga attgccgctt
     6301 cgccattctg cccgatgatg agctgactga tgaccggccc gatctgctta accatcggca
     6361 ggatgccttc catgtccgga ttcgcttgca cggcgacaac caacgaaatc ggttggaccg
     6421 cctcttccac ccgaatgtcc tgcaacttgt tgttatcgta taagcggaaa tcttccggct
     6481 tcagcccggc aacatagttt ccctgcttat cggtaatggt gaccgggacc accaccacgt
     6541 ttacagtggc agtgaaagtt ctctcgggca actcttcttg ggcaaaaaga gcgaagcaca
     6601 ggagaatagc tagaattgct cggaaaacat tcatagttca accgttggac gtcctttcgc
     6661 cggcttcagt ttcgaatacc ggagtggtcc gtgtggccac gcagaagttc gagaatgtgc
     6721 ggcaccaggt cgacgatggc gttcagagac tcgactgcac cctgtggcga acccggtaaa
     6781 ttcacaatca gcacgtgacc gcgcgtgccg gcaaaactgc gcgacaaagc agccagagga
     6841 gtggacttgc gtccttcaaa gcgcattagc tcgccgagtc cgggaatttc ccgatcggcg
     6901 atccggcgtg tggcctcggg agtaacatct cgaggagcaa tgcccgtacc acccgtagta
     6961 aagatagcgg atagctgatt tccgtccgcc agcgtagcca gccgcccggc gatggcgtcg
     7021 gcctcgtccg gcaacacttc ggttgccgca atggaccagc ctagttgctc caggcgttct
     7081 ctaacggcag ggcccgatag atccaacctc gaaccgcgat gtaccgaatc gctgattgtg
     7141 agcacggctg cttgtatcat cgttcgattc tcccggatcg cgcggacatg attattcgat
     7201 gaatccaagc tcacgcaggg atctttcggg ctccgcccct tcgaaatgcg ggaaggcggc
     7261 tttttgcctt ccgtcttcca aagccacgcc ataaggagtg ctggcgaacc ggaacgtctc
     7321 acgcagcttc tcaatgtccg tcgatagatc ggccttgagt ccggtggaag ctaagaactg
     7381 atcggcgaat tggggcattg cagtggctat tgcaatgata cgcgtgtcac gccagcggta
     7441 gccggacatc tccttcgccg cgaaaacaca gtgtgagcat tccggatcga aaaaatagag
     7501 aaaaaatcgc ccatcgtcca gtgcaacagg ctgaccggcc acctggatgg tggagggagc
     7561 gcgaagtcca ctctctctcg cgaccgtcat tccgtaagag actccagcga acacgcagac
     7621 ggccgtcaga ataatggcag ctccgcgttt tgattccgat ggacgcgccc atactccagc
     7681 gaaccaggcc atcgcgagca tcaccagatc tccgatgatg aacgccggac caacagttcg
     7741 tttgatccac ggaaaacaat tgcactcttc gccacgcagg gcgttgtagt tgatggcgaa
     7801 ataaccgagg aacgcaatca aaagcagccc gctaaggcag gcaccccagc gccggtaacg
     7861 tggcaccagc agcagcacac cggcgaacgt ctccgagatg ccaagcgaaa gggcggcggg
     7921 cagactgaac cacgcgggca gcagagcttc cctcatgcgt gcagcggcgc tgagcgggtc
     7981 cgtgattttc caggtgcccg ccacaatgaa cgccagcccc aacagcactg cacagcagag
     8041 gctcaaaatg gttttccacg ccggcacctc ccagggagcg gttcggctcc ggggatgtgc
     8101 catactgtgt tccatgcttc tcattctagc cctgctaacg gccctggtgc ggcctggtgg
     8161 gcgcctggaa gccgagttgc cgcgggttac catcatcgac gacacggtgg agatcgccgc
     8221 ttcaaaagtg attggatttc cgcttctgtt acgtcaaagc gcggctgtcg tcgagtgctc
     8281 ctatgccgtg gacgaggggg gatccggcgt gcgcgttctg ttgcttcgcg cggccgacgc
     8341 cgaggaactg tattcgggcc acgcacaccg cccgctcatt tccacgccct atcagcgcag
     8401 cggaggattc cggtatcgcg tgccgttggc cggagattac gtgctggttc tggataaccg
     8461 gatggaaggg cgcggcgtct ccaaagttcg tctacatcta tccgccgtgt ttggccaggc
     8521 ggcgcgggaa acagtccgct atcttccgag aagccggcgc attgccgtag tcgtcatcag
     8581 cctgctggtc ttcgcgtcta ttgtgactta ctcaggacgg aaactcgctg ccgctatggc
     8641 tgctcgaaga agcggcgggc cgcctgcttt gtacggataa tgtcatcggc ggaatgtgcc
     8701 gctgaaacaa atcccgcctc gaactgggaa ggcgccagat agatgccgtt ctcaagcatc
     8761 cagtggaaga agcgtgcgaa ttgggccgta tccgatcgct tggcggagtc ataatcagta
     8821 accggacggt ccgtgaagaa aaacgtgaac atcgaaccga tgcgattcac tgtcacgccc
     8881 gcaggcgcgc cctcgacgag ttccgccgtt ctcgcttcga gttgctcata aatccaggga
     8941 ttctcgcgaa gatgccggag catggcgagg cccgcggcga cgccgagcgg attgccggac
     9001 agagtgcccg cctgatagac gggacctaaa ggcgaaactt gcgacatgat gtccttgcgt
     9061 ccgccatagg caccgatcgg cagtcctccg ccgatgatct tacccagcgt cgtgagatcg
     9121 ggacggattc cgtagagttg ctgtgctcct ccgaacgcca cgcgaaagcc cgtcatgact
     9181 tcatcgaaga tcaacagcgc gccatgccgg ctggtgagat cccgaaggct ctccaaaaat
     9241 ccgggcaccg gaggtacaca tcccatattc ccggctacgg gttctacgat cacggcggca
     9301 atctcgccgc cgcgctggga aaacacgctc tccacggccg caatcgagtt gtagggcaca
     9361 gcgatcgtgg tatcggagaa agctttggga acaccggctg tgtctggaat cccaaatgtc
     9421 gcgacaccgg atcccgcctt caccagcagc gaatcgacat gcccgtgata gcaaccttcg
     9481 aatttgacgg tgacatcccg gccggtgaag ccacgcgcga ggcgcagtgc gctcatggtg
     9541 gcttcggtac cggaattcac caggcgcacc atttcaatgg acggcaccgc cgcgcagatc
     9601 tcctcggcca gctccacttc aagctccgtc ggagccccaa aactggtgcc gtgttccagc
     9661 gccttttcaa cgccggcgat aatagccggg tgacggtgcc cgaggatcag cggaccccac
     9721 gagcccacat aatccacgta ttcattgccg tccacatcaa agacttttgc cccttcaccg
     9781 cgaagaataa agggcggtgt tccgccgacg ctcttgtagg cgcgaacggg cgagttcacg
     9841 ccaccgggaa ttacattgcg cgcgcgttcc aacagaacat cggaacggtc cagacgcagc
     9901 ggggttgtgt tgcgggagtg aggcattctc ttttatattt tgttctcgcc tgtgaccagg
     9961 cggccgaaga agaagttcat tactacttcg tgcaaggtcc cgttcagatt gcgcaacagc
    10021 cgccctttca gatcgctgta tggctgggtg ccggcgaaaa ggtcctgcat cagatcgtgg
    10081 aatcgcggac tccgcctcat gaactggatc atgcgcgccg gtacggtgcc gaagaggaac
    10141 cggcctacga aaacacggcg ggcgaaggtt gcgctgaagg cgaggtccgc cgcaaaatcg
    10201 cgatggagaa ccgtgcgata ggcggcggtc atcgcgctgc ccggatgccg ttcctcgacg
    10261 aggatccggc tggcaagatc tccggagcgc atcgcataat acaggccttc acccgtgatc
    10321 gggtcgacca gtcccgcggc atcgcccacg gcaagccagc catctccggc gatgtggttc
    10381 gttttccacg ccggtgtggc aagcgacggg aggacatggc tatagaaagc tgcgttctgc
    10441 caggaaatac aacgcagcct catgtagtct tccagccgct tccgcaacga ttgggcggat
    10501 tcgcctttgc cgcagatacc gaccgacagg tgcccacagc ggggaaagac ccagatgtag
    10561 ccctccagct tgggaagaaa ctgaatgtcg atgtgttcct gcatggatgg aacatagtag
    10621 ccaagcgcga acatcgtgtc gttgcgagtc cattgcgtac ccacctgccg caacgaattt
    10681 cttgcaccgg tggccacaat gcagaagtcg gcatcgatga cgccgcccga cgtcctgata
    10741 cgccagcgcg aagcgccacg ggccatttcg agcacacgtg ttttttccag ttgcgcgccg
    10801 gcgcgctcgg cccgttcgag cagcatccgg ttcagatcga agcgggagta gatcaccagc
    10861 ggatgggtga gcttcatctt cacggacccg gcttttggtt ccgaaagata ggtgtcccag
    10921 acgatcttct tgggcttatc gttgtcaatc aggaaagggt attcgcgata agccttgtag
    10981 gtaagaccgc caccgcaagg tttctcccat gccagcttct cgtcgagcag taccgtctcc
    11041 agaccggaag cagccagcct ctcggccgcg aatgccccgg cgggaccacc tccgagtacg
    11101 gcaatacgtt tcatttcttc ggctgtgctt gctcgttcgg agattgcggc ggaaccggag
    11161 ggtgtcctgc aggcagatcg ccggatcccc cggcgggagg catgtccgat ccaataccgc
    11221 catgcgggtc gcttcccgaa cccgcgccgc gccggccctt cactacccac ttgtcgcctt
    11281 gccgttccag cgtgtacgcc atttgcatac ccgccgcggg atcgttggat cctttcgcgc
    11341 ggaaggaaac cgtcgcatca gcctcgttct cacggaaggt gacggacgtg acctctacct
    11401 gcatcgagct cacatccagc cccgaccggc tggagagata atcgataaca cttcggcgta
    11461 tggcttccgt gttctgtatg tcgcgccggc agccacccag caacagaaaa gccgccgcga
    11521 ggacgacgag ataaactttc acgttccgat tatagcctag aggttttgcg attccggcac
    11581 ggctcacttc aacgtggata cccactcccg aacggtccgg gccatttcaa ggaacacatc
    11641 ttctttgctc tctcgcgccc gcttcagcgg gcgaaagccg tggtccgcgg tgtccagcgg
    11701 gtggagcgta gcgcgatcgc cgattttgtt gcagacggtc cgcagcaggt ccggttcggc
    11761 cagcgcatcg cgcgttccag tgagaaacag catcgggatc tggactgcgc cgaggtggtc
    11821 cgcgcgcttc gtatccggtt tgccgggctg atgcagtgga aatgcgaaaa agacgatacc
    11881 acgcacgcca tcgagcggct gcccggaggc cgccgtcgaa gtcatgcgcc ctccgaaaga
    11941 gtggcctccg gcgagcaggg ggagaccggg cgccgctctg tgcgccgccg ccaccgcgga
    12001 gcgcaccgtc gctgtgcagg tatcctggga gtcacgcccc cggccatttt ccatgtaagg
    12061 aaagttgtag cgaaaggtgg ctatattctc accggccagg ctgtccgcga tggactggag
    12121 gatacggtgc cgcatgttcg tgctggctcc gtggccgagg acgagaaggt gagtcgcatt
    12181 ctccggacgg atcagcagcg aagagacttc ccccttctcg ggcgtagcag caaaccgcgc
    12241 ctctgttgtt gggtgagcca ttcaccttct caggcggatc ccgtaggttt acttttcgaa
    12301 gtggaaatcc gctacgaagg cactgcgctt tccaggattg ctccaggttg ttcccaatcc
    12361 ttcgccgtta aagctgtggg ccagggtaaa cgcaggcatc gcgtgatcca ctttcgtgag
    12421 cggggtgtac cagacttctt cgttatggca aatctgatcg ccgttcttca aggcgcgcgt
    12481 ttcgattgac gcgagattgc cggctttcag tgtcgctgta gccgtatgga cattaccgtc
    12541 cttcacggca aaagaaatcg gctggtactc gatgcgaaca atctcctgca gaagatcgcc
    12601 ggacattccg cgtgcaaact ccttcaatgc gttccgttgt gccgcacccg cccgttcatc
    12661 cacgatcagg acggatttaa cgggatatgc ggattggaaa ccgtcaccca gcgtgtggct
    12721 tgcttttaac acaccgacta cggagagccc gtcgagattg acaccgttcc aactgccctt
    12781 ctcgatacgc cagcccatta cggccagatc gcccaccaat cccacttcgc cattggcgaa
    12841 acacggtccg gtatatacgt cggccgtgcg ggcctcgatg tattcgcctt gaatttgtgt
    12901 ggacggaacc gaagcgccag acactcccgt ggaaatcagc aatgcggcaa gagctgtccc
    12961 agtaagaact ttcatcccag atcctccaag taattctttc ttcagtctat cactagtgtg
    13021 ctgtcacgga agttcgcttc aaaaagtcac gaccgcttgc ttgcgcgcgc ggctccgtca
    13081 cgcctttgtt tctgagccgc gacggttacg gagtggttgt gcgcgtcgaa caccagatcg
    13141 gcgcggccga taaacaaagg accgccccat cctaacagga ggcggggcgg tgattccagg
    13201 agatccaaca gatcttacag cgggtggtag cagccgcggg tctcgttgca ctcgtaatcg
    13261 tccacgagtt ta
//
LOCUS       DASTMK010000590         9256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_573218, whole genome shotgun sequence.
ACCESSION   DASTMK010000590 DASTMK010000000
VERSION     DASTMK010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573218"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1495
                     /locus_tag="VFQ79_24300"
     CDS             215..1495
                     /locus_tag="VFQ79_24300"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0142865.1"
                     /translation="MEVVYPRCCGMDVHKATVCACINIKSEGGGTEKEKRRFETTTEE
                     LQALAAWLRQGQVATVAMEATGVYWKPVWNALEQEFELLLVNPQHLKAIPGKKTDFRD
                     GERIADLLQHGLLQGSFVPPRPIRELRDLTRTRASFAQERSRVINRIEKTLEDPHIKL
                     GVVASDVVGVWGRAMLEAMTEGESDPDKLAEWAKGTLRKKIPQLRLALQVNFTDHHRF
                     LLRQWLDTLDFVDRKLALLDQQIAEKVRPLESTIVQWTRVPGLRGTERKRGERKLINA
                     YSLLAEIGDGLTRFRSAGHLASWACLCPGNRESAGKQASGRTRTGNRWLRRTLCEAAW
                     AASRTKRSYFGALYRRLVRGRGKNRALIAVAHSLLVTIYTITVSGTPYRDLGDDYYER
                     LNRKHLERSLLKRLEKLGNEVTMKPKAQPLSAPA"
     gene            1619..2575
                     /locus_tag="VFQ79_24305"
     CDS             1619..2575
                     /locus_tag="VFQ79_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0142866.1"
                     /translation="MMAVHLENGRITLQRRPKPRRRDGYALIRLLSAGICNTDLELQR
                     GYYGFSGIPGHEFVGEVVESDNPQLAGRRVVGEINLACGKCEWCARGLGRHCPRRAVL
                     GIVKHPGAFRELIDLPEANLHLVPENVPTEQAVFIEPLAAACEILDQVKIPRGSRVGV
                     LGDGKLGLLVGQVLLAHGAEVHQYGRHKEKLKIAETAGAETHMANGKLPRAAYDFVVE
                     ATGSQEGLKQAVAMTRPRGTLVMKSTVHGTVAIDTAPIVVNEITMVGSRCGRFEPALR
                     LLRERKVRVEDLIAEVLPLAEAPRAFQHAATRGVLKVLLATA"
     gene            complement(2572..3030)
                     /locus_tag="VFQ79_24310"
     CDS             complement(2572..3030)
                     /locus_tag="VFQ79_24310"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0142867.1"
                     /translation="MAAKCKDLMTKDPVCCLSSDSAEKAAQTMKREDIGSVPVVEDSQ
                     SKKLVGIVTDRDLALKIVAEGRDPKSARVSDVMSRELVTCSADDDAQNAIDAMEDHQI
                     RRLPVVDTTGRIVGIISQADIAQRLGKDEETGKMVGEISKPKRMGGGGAR"
     gene            complement(3123..3854)
                     /gene="lptB"
                     /locus_tag="VFQ79_24315"
     CDS             complement(3123..3854)
                     /gene="lptB"
                     /locus_tag="VFQ79_24315"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEU0142868.1"
                     /translation="MPVLQTRQISKAYRGRKVVDNVSVEVAKGEVVGLLGPNGAGKTT
                     SFYIIVGLVSPDSGQILLDDTDITNLPMYQRARLGISYLPQEPSVFRKLTVEDNLMAI
                     LETLRIPARERRERMMRLVAQLGLEKVRRNKGYMLSGGERRRVEIARSLVIEPSFLLL
                     DEPFSGIDPIQVLELQNIIFELKRSGIGILVTDHNVRETLAVTDRAYIINNGAIFRSG
                     TPETLGSDSDVKRVYLGETFTFETR"
     gene            complement(3859..6126)
                     /gene="lptC"
                     /locus_tag="VFQ79_24320"
     CDS             complement(3859..6126)
                     /gene="lptC"
                     /locus_tag="VFQ79_24320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEU0142869.1"
                     /translation="MWRARQLILLAILVIVLAVAAVYYLQKAVLARRAPQRPTPIASN
                     LNASAEDWVWSKTEDGRPVVEVRARTFEQVRDPAVFNLEGVELRLYEKDGKTYDLVKS
                     ARAQFDQSQNVLFSEGEVEITMGIPVEDQTNGRLLVIRSSGVRFDTKTGKAETERPAA
                     FVFDRGEGQSVGASYDPGSRELWMRGHVQLFWHSDGPGNKEMKVEAGELVYKEAESKV
                     YLSPWSRLTRGEMLLEAANSMVTLENGAIRMVDAQDARGHDKYPSRQIEYAASQLHMD
                     FADDMRIAKISGSGFTRLVSRTSGAATTVTSERLDLLFDPAGEESILTAAHANGKAVA
                     TSQPVPRTGVLTPDTRILRAEAIELTMRPGGQEMEKLATHTPGTIEFIPNREGQPRRN
                     MDADRMTILYGPDNQVQSFRAVNVATRTERPQRKPAPKQSAVSPALTWSKDLAAEFDP
                     ATSQIKKLEQWGDFRYQEGAQQATADHAALAYPDNIIVLSGAARVWDPSGSTSGAKIL
                     LNQQTNDFAAEGNVSSTRLPEKKRAESAVLAANDTMQAKAERMSSAKNNQTIVYEGKA
                     VLWQGANRLEADRIEIDRESDRLYARGNVVSRFVDQSGDKPGKDKRTAPPVPVFTVVQ
                     ASELAYSDKEKLAHYTGGVTLRRPDMEVKAGEIRAFLKEEKGGGSSLDYALADTNVRI
                     FQQAPDRTRTGTGEHAEYYLDDGKVILEGGTPLLVDSLRGSTTGEKLIYFSGDDRLLV
                     NGAERQPVKSILRRK"
     gene            6385..6696
                     /gene="groES"
                     /locus_tag="VFQ79_24325"
     CDS             6385..6696
                     /gene="groES"
                     /locus_tag="VFQ79_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU0142870.1"
                     /translation="MNIRPLYDRIVVKRIEETETVQGGIIIPDTAKEKPQEGEVVAVG
                     KGKRLEDGKVVPLDVQAGDRILFGKYSGSEIKIDGNEYLIMREDEVLGVIESAGKTAK
                     A"
     gene            6715..8346
                     /gene="groL"
                     /locus_tag="VFQ79_24330"
     CDS             6715..8346
                     /gene="groL"
                     /locus_tag="VFQ79_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU0142871.1"
                     /translation="MAAKQIVYSENSRQAILRGVNQLADAVKVTLGPKGRNVVLEKKF
                     GGPTITKDGVTVAKEVELKDPLENMGAQLVREVASKTSDIAGDGTTTATILAQSLFRE
                     GVKAVAAGANPMALKRGIERAVELVVEEIQKFSKPVSGEMIAQVGTISANGDSTIGNI
                     IAEAMQKVGKDGVITVEESKTMSTELQTVDGMQFDRGYLSPYFITDPDRMEAVVEDPY
                     ILIHEKKISNMKDLLPLLEQIARSGKPLLVIAEEVEGEALATLVVNKLRGTLNCCAVK
                     APGFGDRRKAMLEDIAILTGGKAIMEETGIKLEGVRLEDLGRAKRVTVDKDNTTIVDG
                     GGQQKTIEGRIKQLRAQIEETTSDYDREKLQERLAKLAGGVAVIKVGAATETEMKEKK
                     ARVEDALHATRAAVEEGIVPGGGVALLRAAKKLEGLKLDPDEQIGVDMVRRAAEEPLR
                     QIVGNAGFEGAIVAEKVRLNEDANYGFNAATSQYTDLVKDGVVDPTKVARTALQNAAS
                     ISALMLTTEAMIAEIPEKKQPPMGGPGGHGPDMDY"
     gene            8470..>9256
                     /locus_tag="VFQ79_24335"
     CDS             8470..>9256
                     /locus_tag="VFQ79_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0142872.1"
                     /translation="MRIVDIRPTTVTVPLEAPLRHANGCHWGRFVRTIVEVEADNGLV
                     GLGEMGGGGESAEAVFRAMKAYLTGHDPVRLEEMRFLIANPTASLYNNRTQILAALEF
                     ACLDLLGQAWGVPVCDLLGGRLRDRVPFASYLFFRYPNPSDGSGEVRTVDQVIANARS
                     LKERFGFTTHKVKSGVFPPDYDLECYRAVAETFPADRVRFDCNAVWSTEQAIRFGQAI
                     EGLNNDYLEDPVYGMNGMRRVREKVRVPLATNTVVVGFEQLAAN"
BASE COUNT         1906 a         2602 c         2923 g         1825 t
ORIGIN      
        1 ggctcacaac ctgagatgaa agtgggtgta ggttgtgatg agacgcggct gcggatcgag
       61 gccgtaaacc gcaacagggc agtgaagcct gccagcaagt ttggtctggg caccgcttgg
      121 aacaacggaa tgtgaccgta gaaatacgga gcacgaaggg gagattcaac tttacctggt
      181 gcctttcgcg gcgcgcgtga gaaaggagaa ggacatggaa gtggtgtatc cgcgctgctg
      241 tggaatggac gtgcacaagg cgaccgtgtg cgcttgtatc aacatcaagt ccgaaggagg
      301 tgggacggag aaggaaaaac ggcgtttcga aaccaccacg gaggagctac aggcactggc
      361 ggcgtggttg agacagggac aggtcgcgac ggtagcgatg gaggcgaccg gagtctattg
      421 gaagccggtg tggaatgcgc tggaacagga gttcgagttg ctgttggtga atccgcagca
      481 cctgaaggcg atccccggca aaaagacgga cttccgggat ggggagcgta tcgcggacct
      541 gctgcaacac ggattgttgc aaggaagctt tgtgccgccg cgtccgattc gagaactgcg
      601 ggatctgacc cggaccaggg ccagctttgc gcaggagcgg agccgggtga tcaaccgtat
      661 cgagaagacc ttggaagatc cccatatcaa actgggggtg gtggcttccg acgtagtggg
      721 agtctgggga cgggcgatgc tggaagccat gaccgaggga gaaagcgacc cggacaagct
      781 ggccgaatgg gcgaaaggga cgctgcggaa aaagatcccg caactgcggc tggcgctaca
      841 agtcaacttt acggatcatc accgattcct gctgcgtcag tggctggata cactggactt
      901 cgtggatcgc aagctggccc tgttggacca gcagattgcg gagaaagtcc gccctttgga
      961 atcgaccatc gtccaatgga cgagggtgcc gggtttacgc ggtaccgagc gcaagcgcgg
     1021 agaacgcaaa ctgatcaacg cttacagtct gttggccgaa atcggtgacg gcttgacccg
     1081 ttttcgctct gctgggcatt tggccagttg ggcctgcctc tgtccaggaa atcgggagag
     1141 cgccggcaag caggccagcg gacgcacccg aacgggcaat cgctggttac gccgcacgct
     1201 gtgtgaggcg gcctgggcgg cctcccgcac caaacgatcc tacttcggtg cgctgtaccg
     1261 ccgattggtg cgcgggcgcg gcaaaaaccg agctctcatc gccgtcgcgc atagcttgtt
     1321 ggtgaccatc tatacgatca ccgtttccgg tacgccttac cgggacctcg gcgacgacta
     1381 ctacgaaagg ctcaacagga agcacctgga aaggtcgctc ctaaagcggc tcgagaaact
     1441 aggcaacgaa gtcacaatga aaccgaaagc ccaacccctg tctgcacctg cctgatactt
     1501 tcaggggagc ggttttcgcc cttttcagcg gccagctagg gcatggcacc gcccgcgtgg
     1561 cgtatcctgg gggcatgtcg cgacagatcg ggtccgggag tagtggagtg tgtacgcgat
     1621 gatggccgtt catctcgaga acggccgcat cacccttcag cgccgcccga aaccgcggcg
     1681 gcgtgacggc tatgcgctca tccgcctgtt gtcggcgggc atctgcaata cagacctgga
     1741 actccagcgc ggctattacg gcttcagcgg catcccaggg cacgaattcg tcggcgaagt
     1801 cgtggagtcg gataaccccc aactggccgg ccgccgtgtg gtgggtgaga tcaatctcgc
     1861 gtgcgggaag tgcgaatggt gtgcgcgcgg gttgggacgc cattgcccgc ggcgcgctgt
     1921 gctgggcatc gtcaagcacc ccggcgcatt tcgtgagctg atcgacctgc ccgaagcgaa
     1981 cttgcatctt gtacccgaaa acgtgcccac agagcaggcg gtgttcatcg aaccgctggc
     2041 ggcggcctgc gagattctgg accaggtcaa gatcccgcga ggctcacgcg taggggtgct
     2101 gggagacggc aaactcggct tgctggtggg gcaggttctg ctggcgcatg gcgcggaagt
     2161 acaccagtac ggcaggcaca aagagaagtt gaagattgcc gaaaccgccg gcgccgagac
     2221 gcatatggcg aatgggaaac tgccccgcgc ggcctacgat ttcgtggtgg aggccaccgg
     2281 ttcgcaggaa ggactgaaac aggcggtggc tatgacgcgg cctcgcggca cgctggtgat
     2341 gaagtccacc gtgcatggca cagtcgccat cgatacagcg cctattgtcg taaacgaaat
     2401 cacaatggtt ggttcacgat gtggccgttt cgagccagcg ctgcgacttc tcagagaacg
     2461 gaaggtgcgt gtagaggact tgattgcaga agtgcttccc cttgctgagg cgccgcgcgc
     2521 gttccagcac gcggcaaccc gcggcgtatt aaaagtactg ctcgcgaccg cttagcgcgc
     2581 gccgccgccg cccatgcgct tgggcttcga gatctcccct accattttgc cggtttcctc
     2641 gtctttcccc aaacgctgcg caatgtcagc ctgtgagatg atacctacaa tccgcccggt
     2701 ggtatccacc acgggcagtc tccggatctg gtgatcttcc atggcatcga tggcattctg
     2761 ggcgtcgtca tcggccgagc aggtcacgag ttcccgcgac attacgtccg aaacacgcgc
     2821 cgatttcggg tcgcgccctt cggcgacaat cttcagagca agatcccggt ccgtaacgat
     2881 tccaaccaac ttcttgctct gactgtcttc gacaacgggg acggatccga tgtcttccct
     2941 tttcatggtc tgtgcggctt tctcggccga gtcgctgctg aggcagcaga cggggtcttt
     3001 cgtcatgagg tctttgcatt ttgcagccat tttgttccct cctcgtgtga agccaaccgg
     3061 catccaaaag tgagactcgg agatagctgg agtgtttcgg gatttgggtg agggcgggaa
     3121 ctttatcgcg tttcgaaagt gaaggtctcg cccaggtaaa cgcgtttcac gtccgaatcg
     3181 ctgcccaaag tttcgggggt tccggagcgg aagatcgcgc cgttgttgat gatataagcg
     3241 cgatcggtaa cggcgagcgt ctcgcggaca ttgtgatcgg tcaccaggat gccgataccc
     3301 gagcgcttga gctcgaaaat gatgttttgc agttcgagca cctgaatcgg atcgataccg
     3361 gagaacggtt cgtccaggag cagaaagctc ggctcgatca ccagcgagcg ggcgatttcg
     3421 acgcgccgtc tttccccgcc ggaaagcatg tagcccttgt tgcgacgcac cttttcgagg
     3481 ccgagctgcg cgacgagccg catcattctc tcgcggcgtt cgcgcgccgg aatgcgcagc
     3541 gtttcgagaa tggccatcag gttgtcctct accgtcagct tgcggaacac cgatggctcc
     3601 tgtggcaaat agctgatgcc gagccgcgcg cgctggtaca tcggcaagtt ggtaatgtcg
     3661 gtgtcgtcca gcaggatctg cccggaatcc ggcgaaacca ggccgacaat gatgtaaaac
     3721 gaagtcgtct tgccggcgcc gttcggcccg agcagaccga ccacctcgcc ctttgcgacc
     3781 tcgacagaca cattgtcgac tactttgcgc ccgcggtagg cctttgagat ctggcgggtt
     3841 tgtaatactg gcatgcggtt acttgcgccg gagaatactt ttgacgggct ggcgctcagc
     3901 gccgttaacc agcagcctat catcccctga gaaatagatc aatttctcgc cggtggtgga
     3961 ccctcgaagg ctatcgacca gaagcggcgt tcccccttct agaatcactt tgccgtcgtc
     4021 cagatagtat tcggcgtgct ctccggtgcc ggtgcgcgtg cggtccggcg cctgctgaaa
     4081 aatccggaca ttcgtatcgg cgagcgcata atcgagactc gagcctccgc ctttttcttc
     4141 ctttagaaac gcccgaatct caccggcttt cacttccatg tccggacgcc gcagcgtgac
     4201 tccacccgtg tagtgcgcca gcttctcctt atccgaatag gccagttcgg aagcctggac
     4261 cactgtaaat acgggtacgg gcggcgcggt gcgtttgtct ttacccggtt tatcgccgga
     4321 ctggtccacg aaacggctga cgacattccc gcgagcgtac agccggtcgg actcgcgatc
     4381 gatctcgatg cggtcggctt ccaggcggtt cgcaccctgc cagaggaccg ccttgccctc
     4441 gtagacgatg gtctgattgt ttttggcgga ggacatgcgc tccgctttcg cctgcatcgt
     4501 gtcgtttgcc gcgagcaccg ccgattcggc acgctttttt tccgggaggc gtgtggagga
     4561 gacattgcct tcggcggcaa aatcgttcgt ttgctgattc aggaggatct tggctccgct
     4621 ggtggagccg gaaggatccc aaacccgtgc ggcgccggaa aggacgatga tgttgtcggg
     4681 ataagccagc gccgcgtgat ccgcggtagc ctgttgcgct ccctcctggt agcggaaatc
     4741 gccccattgc tccagctttt tgatctggct ggtggcgggg tcgaattcgg ccgcgagatc
     4801 tttgctccac gtcaaggcgg gagacacggc agattgcttc ggtgccggct ttcgttgcgg
     4861 acgctcagtg cgagtcgcca cgttcacagc ccggaaggac tgaacctggt tgtccgggcc
     4921 gtaaaggata gtcatgcggt cggcatccat gttgcggcgc ggctgccctt cacggttcgg
     4981 aatgaattcg attgtgccgg gcgtgtgcgt cgcgagtttt tccatctcct gcccgccggg
     5041 acgcatggtc agttcgatcg cttcggcgcg caggatccgc gtgtcggggg tgagcacgcc
     5101 ggtgcgcgga accggttggg acgtggcgac agccttgcca ttggcgtgag cggctgtaag
     5161 aatactctcc tcgccggcag gatcgaacag caggtccagg cgttccgaag tgaccgttgt
     5221 cgccgccccg gacgtacgtg aaaccagccg ggtgaatccg ctccccgata tcttggcgat
     5281 gcgcatatcg tcggcgaaat ccatatgcag ttgcgaagcg gcatattcga tctgccggga
     5341 cgggtatttg tcgtggccgc gcgcgtcctg cgcatccacc attcggatgg caccgttttc
     5401 gagggtcacc atcgagttgg ctgcttcgag cagcatttct cccctggtca gccgcgacca
     5461 gggggaaaga tacactttcg attccgcttc cttgtaaacc agttcgccgg cttcgacctt
     5521 catttctttg ttgccaggtc cgtcgctatg ccaaaacaat tgcacatggc cgcgcatcca
     5581 cagttcgcgg cttcccggat cgtaggaagc gcccacggac tggccctcgc cacggtcgaa
     5641 gacgaaagct gccggacgct cggtttccgc cttgcccgtt tttgtatcga accgcacgcc
     5701 ggacgaacgg atcaccagca gccgcccatt cgtctgatct tcaacgggaa tgcccatcgt
     5761 gatttcgact tcgccctccg agaaaaggac gttctgtgac tggtcaaact gggcgcgggc
     5821 gctctttacc aggtcgtagg tctttccatc tttctcatac agccgcagtt cgaccccttc
     5881 cagattgaaa acagcggggt ctctcacctg ctcaaaggtc cgggcgcgaa cttcaacaac
     5941 cgggcgccca tcctccgttt tggaccagac ccagtcctcc gcgcttgcgt tcaggttcga
     6001 ggcaatcggg gtaggacgct gcggggctcg ccgggcaagg acggctttct gaaggtagta
     6061 gacggcggcg acggcaagga caatgaccaa aatagccagc aggatgagct ggcgcgcgcg
     6121 ccacatgtgc ttccaggata gaacagatcc cgtgccggat tcagtagacg ggcgggaggt
     6181 ggaaacgcgg gggccttcaa tattcggcgg gacagtttgt caagaaataa aaacccttcc
     6241 gtaacagcaa tttatgcaag tttattgtca ctcctcgggt gagtgtgcta ataatagcta
     6301 tcgggttcca gaaatacaaa actgaaaccc aaagcctgaa agaacaacca gacatacatc
     6361 ttactggagg tccagagtca gagtatgaat attcggccgc tgtatgaccg cattgtggtc
     6421 aagcgcattg aagaaacaga gaccgttcag ggaggcatca tcattcctga cacggcgaaa
     6481 gagaagcccc aagaggggga agtggttgct gtcggaaaag gcaagcgcct ggaagacgga
     6541 aaagtcgttc cgctggacgt gcaagcgggc gatcggattt tgttcggtaa gtactccggg
     6601 agcgagatca agatcgacgg aaacgaatat ctgatcatgc gtgaggacga ggtccttgga
     6661 gttattgaga gtgccggcaa gacggctaaa gcgtaacgat caggagagga atatatggca
     6721 gcaaagcaga ttgtttacag tgagaattcc cggcaggcca ttctgcgggg ggttaaccag
     6781 ctcgccgatg ccgtgaaagt caccctgggg ccgaaaggcc gcaacgtcgt gctcgagaag
     6841 aagttcggcg gcccgacgat cacaaaagac ggcgtcaccg tggcgaaaga agtcgagctg
     6901 aaggatccgc tcgagaatat gggagcccag ttggtgcgcg aggttgcatc gaagacgtcc
     6961 gatatcgcgg gcgacggcac gacgacggcc accattctgg cccagtcgct gttccgcgaa
     7021 ggcgtgaagg cggttgcagc cggtgccaac ccgatggccc tgaagcgagg tatcgagcgg
     7081 gccgtcgaac tggtggttga agagatccag aagttcagca agccggtgtc gggcgagatg
     7141 atcgcccagg tgggcacaat ctccgccaac ggcgattcca ccatcggcaa catcatcgcg
     7201 gaagcgatgc agaaggtggg taaggacggc gttattacgg tggaggaatc gaagacgatg
     7261 tccaccgagc ttcagaccgt ggacggcatg cagttcgacc gcggctacct gtccccgtac
     7321 ttcatcaccg atcccgaccg catggaagca gtcgtcgaag atccttacat cctgatccac
     7381 gaaaagaaga tcagcaacat gaaggatctg ctgccgctgc ttgagcagat cgcccgctcc
     7441 ggcaagccgc tgctggtgat cgccgaagaa gtagaaggcg aggccctggc aacgctggtg
     7501 gtgaacaagc ttcgcggaac cctgaactgc tgtgccgtga aagcgcccgg tttcggcgat
     7561 cgccgcaagg ccatgctgga ggatattgcg atcctgaccg gcggcaaggc gatcatggaa
     7621 gaaaccggca tcaagctgga aggcgtccgt ttggaagacc tcggccgtgc aaagcgcgtg
     7681 acggtggaca aggacaacac caccatcgtc gacggcggtg gacagcagaa gacgatcgaa
     7741 gggcggatca aacagcttcg cgcccagatc gaagagacca cgtcggacta cgatcgcgag
     7801 aagctgcagg agcggctggc gaagcttgcc ggcggtgtcg cggtgatcaa ggtcggcgct
     7861 gctaccgaga ccgagatgaa ggaaaagaag gctcgcgtag aagacgcgct gcatgctacg
     7921 cgtgcggcgg tggaagaagg tattgtgccg ggcggcggcg tcgcgctgct tcgcgcggcc
     7981 aagaagctcg aagggttgaa gctggatccc gatgagcaga tcggcgtcga tatggttcgc
     8041 cgcgcggcgg aagagcctct gcggcagatc gtcggcaacg ccggtttcga gggcgccatc
     8101 gtggcggaaa aagtccgctt gaacgaagat gccaactacg gcttcaacgc ggctacttcc
     8161 cagtacaccg acctggtgaa ggatggcgtg gtcgacccga ccaaagtggc ccgtaccgca
     8221 cttcagaacg ccgcttccat cagtgcattg atgttgacga cggaagcgat gattgccgaa
     8281 attcccgaga agaagcagcc accgatgggc ggtcccggcg gccacggccc cgacatggac
     8341 tactaagccg ctgcgctgaa tctcaaatca ttggccgtcc cgctccaata gggtgggacg
     8401 gcttttttat gtgccgggct gcccgtaaca ccacgctgcg ctccgctgct atgatgcaag
     8461 ctttagagaa tgcgcatcgt cgatatacgt cccaccactg tcacggtccc gctggaagct
     8521 cccctacgcc acgcaaacgg ctgccactgg ggacgctttg tgcgcaccat tgtcgaagtg
     8581 gaagcggaca acggactggt ggggttggga gaaatgggcg gcggaggcga atcggccgag
     8641 gccgtttttc gcgcgatgaa agcgtatctg acggggcacg acccggtgcg cctcgaagag
     8701 atgcggtttc tgatcgcgaa ccccacggca tccctgtaca ataaccgcac gcagatcctg
     8761 gccgccctcg aatttgcctg tctcgatctt ctggggcagg cgtggggtgt gccggtttgc
     8821 gacctgctcg gcggacgttt gcgggatcgt gtcccctttg cttcttatct cttcttccgc
     8881 taccccaatc cttctgatgg ctccggtgag gttcgcacgg tcgaccaggt gattgcgaac
     8941 gcgcgctcgc tgaaggagcg attcgggttt acgacacaca aggtaaagtc gggtgtgttc
     9001 cctccggatt acgacctgga gtgctaccgg gccgttgctg aaacatttcc ggcagatcgc
     9061 gtgcggttcg actgcaatgc ggtgtggtcc acggagcagg caatacggtt cggccaggcc
     9121 atcgagggat tgaataacga ttacctggaa gaccctgtgt acggcatgaa cggtatgcgc
     9181 cgggtccgcg agaaagtacg cgtaccgttg gccaccaaca ccgtggtagt gggtttcgag
     9241 caactggcgg ccaata
//
LOCUS       DASTMK010000591         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_318615, whole genome shotgun sequence.
ACCESSION   DASTMK010000591 DASTMK010000000
VERSION     DASTMK010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318615"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..380)
                     /locus_tag="VFQ79_24340"
     CDS             complement(147..380)
                     /locus_tag="VFQ79_24340"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEU0142873.1"
                     /translation="MEQYQEEIKAHLMATDEEFRRLAIKHSEYKKRVEELESRPHLSD
                     EDHMEEVRLKKIKLHLKDQMLEMISRHRTQQTV"
     gene            complement(510..2627)
                     /gene="rimI"
                     /locus_tag="VFQ79_24345"
     CDS             complement(510..2627)
                     /gene="rimI"
                     /locus_tag="VFQ79_24345"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU0142874.1"
                     /translation="MKNRLKKLWFRLIRKDPEAVVVSFLTGEEPAARAMLQEIRALVP
                     DRRHYAAGFRKPEFLSAEVEFLQLERAPAIRLFFELRRRLRAKRIGIAPLLLDGTAEF
                     TGLRRAAFLYAPLKILAYNKRLERHHLKLSTLIASILFLRGVPLDRIFLRPSWVTPWK
                     RGMPEDDGTHQAYEGRPVSPARRRVAVLTPYFPYPLSHGGAVRIFNLVREIAREFDVY
                     LFSFIERGKPVEMQPMLELCAKMALVPVPRYREPRWSTLHPPEVGEFRARAMKQALRD
                     LFRDDRIELLQTEYTALATYGGDVLVEHDVTFDLYQQILARKRSWTARWDLWRWKRFE
                     TRAFGTYKRIVVMSEKDAKLVDLPAAVRILPNGVDLERFQPTPEAPGERLLFIGSFRH
                     FPNMAGYRFFVEEVWPLLQKRHPAMRLTVVAGPDAALHWRAFTGMAELVPENGIDLRE
                     FERDVVPLYAASNVVIVPTVVSAGTNLKVLEAMAMERAVVSTPSGCAGLGLRHGHSVW
                     VADGAGEFAEGVSRLIQDENLRKQIARNARRFAEENFEWKRIGELQRALFRELLAARD
                     LPVLLREGGPPDLAGIAAIQSACPEASQWEPASYLAASLVVAESGGELCGFIAVRRVA
                     EEEYEVLNLAVAPRFRRAGVGTKLLAHVCERWGSDIFLEVRESNTAARALYGKLGFVE
                     AGIRKKYYDNPSESAVVMRFHSC"
     gene            complement(2624..>3726)
                     /locus_tag="VFQ79_24350"
     CDS             complement(2624..>3726)
                     /locus_tag="VFQ79_24350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0142875.1"
                     /translation="QLAPVLKEEFQLSNQDFGFVLSAFSILYAACAPFAGLFVDRAGL
                     NRAASIAVATWSLAGIGTGLVSGFAGLLGARALLGLAESAGVPASGKAVRLYLKPEER
                     ALGGAVSQLGLSVGSFLAPPIATFLALWYGWRSAFILTGLLGFLWIPLWLATAKRYPP
                     RAEAPSGTTRGAARILRDSRLWAFATANILGMAVYTLWSNWITIFLMQGYGLKLVETP
                     WLAGIPPLVAPLGGFFGGWISLRLVRRGMEAPKSRRMACLVSAIALLGTAAVPWMPSA
                     RLATAAIAISFFWASSYSVNLYALPLDVYGAEHAAFGTSILTCAYGLLQALFSPVIGG
                     MVDRYGFEPVCLLVAILPLVGTGVLKLAEKQQ"
BASE COUNT          783 a         1184 c         1076 g          683 t
ORIGIN      
        1 gatcccggat ttcagttcag caggctaagc aaaatcctgg gcggtcaaaa gatcgacgga
       61 tctcgttaag atagcgaaaa aatgaaaagc ccggcccgga gtatccggac cgggccattc
      121 ggttgttctt tagcgcatcc gatatttcag actgtctgtt gtgtacggtg ccggcttatc
      181 atttccaaca tctgatcttt aaggtgaagc ttaattttct tgaggcgcac ttcctccatg
      241 tgatcctcat cgctcaggtg tggccgagac tccagctctt ccacacgttt cttatactcg
      301 gaatgcttta tagccaggcg acggaattct tcatccgttg ccatcagatg cgctttgatt
      361 tcttcttggt attgctccat ttcagggggt tccctccttg ggtgaggttc gccacgcccg
      421 ggggttgctg acgctaccgg cccggtgggt gagtgtgaac tgcatatgag gttgcggaac
      481 ggtcaccgtg ccgtcccgtc gattcgacat taacatgaat gaaatctcat gacaaccgcc
      541 gattcgctag ggttgtcgta gtactttttc ctgattcccg cctccacaaa accgagcttg
      601 ccgtaaaggg ctcgcgcggc ggtgttcgac tcacgcactt ccagaaaaat gtcgctaccc
      661 caccgctcgc agacatgcgc cagcagcttc gttccaacgc ctgcgcgccg gaatcggggc
      721 gccacggcca ggttcaaaac ttcgtactct tcttcggcta ctcttcgaac cgcgatgaat
      781 ccgcaaagct cgccgccgga ctccgccacc acgagggacg ccgccaaata ggacgcgggt
      841 tcccattgcg atgcttcggg gcatgccgac tgaatcgccg caattccggc aaggtccggc
      901 ggccctccct cacgcaggag aaccggaaga tctcttgcgg ccagcagttc acggaacagc
      961 gcccgttgca gttcgccgat tcttttccac tcaaaattct cttcggcaaa acgccgcgcg
     1021 tttcgcgcga tttgtttccg gagattctca tcctgaatga gccgggacac gccctcggcg
     1081 aactcaccgg cgccgtcggc gacccagacg ctgtggccat ggcgcaggcc gagtccggcg
     1141 caccccgagg gagtggagac caccgcgcgt tccatcgcca tggcttccaa caccttcaga
     1201 ttggtaccgg ccgagaccac cgtcggaacg atcaccacgt tcgaggctgc atagagcgga
     1261 accacgtcgc gttcaaactc ccgcaggtcg attccgtttt ccggcacaag ctcagccatt
     1321 ccggtgaacg cgcgccaatg cagcgccgcg tcgggtcccg ctacgacggt cagccgcatt
     1381 gccggatggc gtttttgcag cagaggccag acttcctcga caaaaaaccg gtacccggcc
     1441 atgttcggaa agtggcgaaa tgagccgatg aacagcaacc gctcgccggg tgcctcgggc
     1501 gttggctgaa accgctccag gtccactccg ttgggaagga ttctcaccgc cgcgggcaaa
     1561 tcgacgagtt tcgcgtcttt ctccgacatc acgacgatgc gcttgtaggt tccaaacgcc
     1621 cgggtttcga aacgcttcca ccgccacaag tcccaccgtg ccgtccacga tcgctttcgc
     1681 gccagaatct gctggtacaa gtcgaacgtg acgtcatgtt ccacgagcac gtccccgccg
     1741 tacgtcgcga gggccgtgta ttccgtctgc aaaagctcga tacgatcgtc gcgaaagaga
     1801 tcgcgcaaag cctgcttcat cgcccgcgcc cggaattcgc ccacttccgg cgggtgaagg
     1861 gtggaccatc gcggctccct gtaccgggga accggaacca gcgccatctt tgcgcacaac
     1921 tcgagcatcg gctgcatctc gactggtttt ccccgttcga taaacgagaa gaggtatacg
     1981 tcaaactcac gggcgatttc ccggaccagg ttgaaaattc gaaccgcgcc accgtgggag
     2041 agcggatagg ggaaataggg cgtcagtacc gctacgcgcc gccgcgccgg cgaaaccggc
     2101 cggccctcgt acgcctggtg cgttccgtca tcctccggca taccccgttt ccacggcgtg
     2161 acccaggaag gccgcagaaa gatgcggtcc agcggaactc cccgcaggaa gaggatcgag
     2221 gcgataaggg tcgagagctt caggtgatga cgttccaggc gcttgttgta cgccagaatt
     2281 ttcaatggcg cgtaaagaaa ggcggcgcgg cggagcccgg tgaattcagc agtaccgtcc
     2341 aacagaagcg gtgcgattcc gattcgcttc gcgcgcaatc tccggcgaag ctcaaaaaaa
     2401 agacggatcg ccggcgcccg ttcgagctgg aggaactcta cctcggcgga gaggaattcc
     2461 ggctttcgga agcccgccgc gtaatgacgc cggtccggca ccagcgcccg gatctcctgg
     2521 agcatggccc tcgcagccgg ttcttccccc gtaagaaaac tcaccaccac cgcttccgga
     2581 tctttgcgaa tcaaccggaa ccacaacttc ttcagtctgt tcttcactgt tgtttttccg
     2641 ccagcttcag cacaccggta cccacgagcg gcagtatcgc aaccagcagg cagacgggtt
     2701 caaaaccgta tcgatcgacc atgccgccga tgacggggga aaagagcgcc tgcagaagcc
     2761 cataggcgca cgtcaaaatc gacgtgccga atgcggcgtg ttcggctccg tacacatcga
     2821 gcggcagcgc ataaaggttc acgctgtacg atgacgccca gaagaagctg atggcgatgg
     2881 ccgccgtggc aagcctcgca ctgggcatcc atggaacggc cgcggtgccg agcagcgcga
     2941 tcgcactgac cagacaagcc atccggcggg acttcggggc ctccattccg cgcctcacca
     3001 ggcgtaagga gatccaacct ccgaaaaagc ctcccagcgg cgcaaccagc ggcggaatgc
     3061 cggcaagcca gggtgtctcg accagtttca ggccgtagcc ctgcatcagg aaaatcgtga
     3121 tccagttgct ccagagcgta tacaccgcca tgccgagtat attggcggtg gcgaaggccc
     3181 acaaccggga atcgcgcaga atacgggccg cgccgcgggt ggtgccggaa ggcgcttccg
     3241 ctcgcggcgg atagcgcttc gcggtcgcca gccatagggg gatccagagg aagcccagca
     3301 atcccgtcag gatgaaggca gaacgccatc cgtaccacag agccagaaat gtggcgatcg
     3361 ggggcgcgag aaaggagccc acgctcaacc cgagttggct tactgcccct ccgagcgcgc
     3421 gctcttccgg cttcaagtac aggcggaccg ctttgccgga ggcgggcacg ccggcggatt
     3481 ccgctaaccc cagcaaggcg cgtgctccca gcaagcccgc gaatcccgag accaggcccg
     3541 ttccgattcc ggccagcgac cacgtggcga cggcaatact ggccgcgcgg ttcaaaccag
     3601 cccggtcgac gaagaggccc gcaaacggcg cgcaggccgc atagaggatg gagaaggcgg
     3661 acagaacaaa accaaagtcc tggttgctga gttggaattc ctctttcagc accggagcaa
     3721 gctgcg
//
LOCUS       DASTMK010000592         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_189871, whole genome shotgun sequence.
ACCESSION   DASTMK010000592 DASTMK010000000
VERSION     DASTMK010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189871"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VFQ79_24355"
     CDS             <1..1309
                     /locus_tag="VFQ79_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEU0142876.1"
                     /translation="IWGDWLDAPVQLVEQGPIDYLILDYLAEVTMSILEKQKQQDPLL
                     GYARDFPPLMHRIARALTERNIKVVANAGGVNPVACAREVKRLAPELTVAVVLGDDIL
                     DRLDELIANGHELKNMDTGEPVTAIRDRVLSANAYIGAFPLAKALATGADAVIAGRST
                     DTALAVAPMIDRFGWVRQDYNRLASGTVAGHIVECGAQATGGNCQVDWQTLPDLANIG
                     YPIVEAEPDGDFTVTKHHGTGGHIDVDVIKEQLLYELGDPKRYITPDCIADFTTIRLR
                     DDGPARVRVGGVRGAPPTETLKVSISYHYGWKATGTLVYTWPDALRKARMADAIVRER
                     LRRLDLKFEEIYTEFLGVDACHGATAPPNDDPPEVQLRIGVRERDKAAVERFTRELIP
                     LVLSGPPGATGFGDGRPQVREVVAYWPALIPRREIATRVEVVE"
     gene            1306..1638
                     /locus_tag="VFQ79_24360"
     CDS             1306..1638
                     /locus_tag="VFQ79_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142877.1"
                     /translation="MKVPLGRIAHTRSGDKGDTANIGVIALKEWHYPTLAREVTAERV
                     KAFFGPLVKGEVERFELPNLGALNFLLHGALGGGGTLSLRVDAQGKTLGAALLRMEIE
                     LDEHEFGL"
     gene            1616..2401
                     /locus_tag="VFQ79_24365"
     CDS             1616..2401
                     /locus_tag="VFQ79_24365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0142878.1"
                     /translation="MNTSLDCSREGHLLRMALNRPDKRNALTSALCRQLLDAIHEADL
                     DDGIGAILLEGRGSVFCAGMDLEEAAQPDAAEQTAIHEELFSIGSRVGKPVIAAVQGA
                     ALGGGMGLLTNAHVVIAAQGTSFGLTEIRIGMWPFVVYRSAVLALGERRALELSLTGR
                     VFGTTEAMQYGLVHQVVPAFELDDRATDVARALAHSSREAMSSGLAYVQQSRNLGWDE
                     AGKLARDLRARLFASADFAEGVRAFKEKRRPQWPSLEGQNAAT"
     gene            complement(2394..>3264)
                     /locus_tag="VFQ79_24370"
     CDS             complement(2394..>3264)
                     /locus_tag="VFQ79_24370"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEU0142879.1"
                     /translation="TDPVTGVTDGILRKAQEQGAVPKIFYTNGSYEYWGRAASLIHTN
                     LEGTQDAKLAPDTRIYLLAGTQHGPGRFPPTKTKAQNTANPADYRPLMRALLVSFQEW
                     LKDGVEPPASEYPLIAKGQLVAVDKLRFPRLAGVEVPKHIQQAYRADYGEEFRSAGIV
                     TKEPPEINGKYMVLVPQVDEGGNEISGIRLPEITVPVASYTGWNLRDPKIGAEDKIYS
                     MVGSFLPFARTKAEREQTNDPRPSIAERYNGRDQYLLRVKAAAGDLVKKRFLLERDVP
                     MLMERAQSLFEFK"
BASE COUNT          605 a          967 c         1043 g          649 t
ORIGIN      
        1 catttggggc gactggctgg acgcaccggt acaactggtg gagcagggcc cgatagacta
       61 tctcatcctc gactacctcg ccgaagtcac gatgtccatt ctcgagaagc agaagcaaca
      121 ggatcccctg ctcggctacg cccgcgactt tccgccgctc atgcatcgca ttgcgagggc
      181 tttgacggaa cgcaatatca aagtcgtcgc gaatgccggc ggcgtcaacc cggttgcctg
      241 tgcccgggaa gtgaagcggc tggcgccgga gctgaccgtc gccgtggttc tgggagacga
      301 tattctcgac cgtctggacg aactcatcgc aaacggtcac gagctgaaga acatggacac
      361 gggcgagccc gtcaccgcga ttcgcgaccg cgtcttgagc gccaatgcct acatcggagc
      421 ttttccgctg gcgaaggctc tggccaccgg cgccgatgcc gtcatcgcgg ggcgctccac
      481 cgatacggct cttgccgtgg caccgatgat tgaccgtttc ggatgggtga ggcaggacta
      541 caatcgtctc gcctcgggta cggtggcggg ccacattgtg gagtgcggcg cgcaagcgac
      601 cggcgggaat tgccaggtgg attggcagac gctgccggac ctggcgaaca tcggctatcc
      661 catcgtggag gcggagcccg atggcgattt caccgttacg aaacaccacg gtacgggcgg
      721 ccacatcgac gtggatgtca tcaaggaaca gcttctctac gagcttggtg acccaaagcg
      781 gtatatcacg cccgattgca ttgcagactt caccacaatc cggctgcgtg acgatggacc
      841 cgcgcgcgtg cgcgtgggcg gcgtccgcgg cgcaccccca actgagacgc taaaagtatc
      901 catcagctat cactatggat ggaaagctac cggcacgctg gtttacacct ggcctgatgc
      961 gttgagaaaa gcccggatgg cggatgcgat cgtaagagaa cgcctccggc gattggattt
     1021 gaaattcgag gagatctata ccgagttcct cggagtggat gcctgccacg gcgcgacggc
     1081 tccgcccaac gacgatcctc ccgaggtgca gcttcgaatc ggcgtgcgcg agcgcgacaa
     1141 agcggctgta gagcgcttta ctcgcgaact catccccctg gtgctgagcg gtcctcccgg
     1201 cgcaacagga ttcggcgacg ggcggccgca ggttcgcgag gtggtggcct actggcccgc
     1261 gctgattccc cggcgcgaaa tcgccacgcg cgtggaggta gtggaatgaa agtgccgctg
     1321 ggacgcatag cgcacacccg ctccggagac aagggcgata ccgccaacat cggcgtcatt
     1381 gcgctcaagg aatggcacta tccaaccctt gcgcgcgaag tgaccgctga gcgcgtgaaa
     1441 gcttttttcg gaccgctggt aaaaggggag gtggagcgct tcgagttgcc gaatttgggg
     1501 gcgctcaact tcctcctgca tggcgcgctg ggcggcggag gcactctctc cctacgcgtc
     1561 gatgcgcagg gaaaaaccct gggggcggcg ctgctgcgaa tggagatcga gttggatgaa
     1621 cacgagtttg gactgtagtc gcgaaggcca cctgctgcgc atggcgctga accggcccga
     1681 taagcggaat gctctgacga gtgcgttatg ccggcagttg ctggacgcga tccacgaggc
     1741 tgacctggat gacggtatcg gcgccattct cctcgaaggg cgcggcagcg tcttttgcgc
     1801 tggaatggac ctcgaggaag ccgcgcaacc cgatgcggcg gagcagacgg cgattcacga
     1861 agagctgttt tccatcggct cgcgggttgg taagcctgtt atcgccgcag ttcaaggcgc
     1921 ggcgctcggc ggggggatgg gcctgttgac gaatgcgcac gtggtgattg ccgcgcaggg
     1981 aaccagtttt ggcctgacgg agattcgtat tggcatgtgg ccttttgtgg tgtaccgcag
     2041 cgcggttctt gcactgggag aacgccgcgc tctcgagctg agtctcaccg ggcgggtttt
     2101 cggaaccact gaagccatgc aatacgggct ggtgcaccag gttgtgccgg cgttcgagtt
     2161 ggacgacagg gctacggatg tggcgcgtgc gctcgcgcat tccagccgcg aagccatgtc
     2221 gagcggcctg gcttatgttc aacaaagccg gaatttgggt tgggacgaag cggggaaact
     2281 cgcccgggac ctgcgggcgc ggcttttcgc cagcgccgac ttcgccgaag gtgtccgcgc
     2341 tttcaaagag aagcggcggc ctcaatggcc gtcgctcgaa gggcaaaacg ccgctactta
     2401 aactcgaata aagattgcgc gcgctccatc agcatcggga catcgcgctc cagcaggaac
     2461 cgtttcttta ccagatcgcc ggcggcggct ttcacgcgaa gcaggtattg atcccgcccg
     2521 ttatagcgct ccgcgatgga cggccgcggg tcgttcgtct gctcccgctc tgcttttgtc
     2581 cttgcgaagg gcagaaagga tcctaccatg ctgtagatct tatcctccgc accgatcttc
     2641 ggatctcgca ggttccaacc ggtgtagctg gcaactggca ctgtgatttc cggcaggcgg
     2701 atgccggaga tttcatttcc gccttcatcc acctgcggca ccagcaccat gtatttgccg
     2761 ttgatctccg gcggttcttt cgtgacgatt ccggcggaac ggaactcctc gccataatcg
     2821 gcgcgataag cctgttgtat gtgcttcgga acttctacgc ccgcgagtct cggaaaccgg
     2881 agcttgtcca cggccacaag ctggcctttg gcgatgagcg ggtactcgga cgcgggtggc
     2941 tctaccccgt ccttcaacca ttcctgaaaa cttaccagca gggcgcgcat caacggcctg
     3001 taatcggccg gattcgcggt gttttgtgct tttgttttgg tgggcggaaa gcggcctggg
     3061 ccgtgctggg tgcccgccaa caggtaaatg cgcgtgtcgg gcgcgagctt cgcgtcctgt
     3121 gtcccctcca gattcgtatg aatcaatgac gccgcccgcc cccagtattc gtatgagcca
     3181 ttcgtataga agatttttgg aacggcgccc tgctcctgcg ccttccggag aattccgtcg
     3241 gttacccctg tcaccggatc ggtc
//
LOCUS       DASTMK010000593        11987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_286565, whole genome shotgun sequence.
ACCESSION   DASTMK010000593 DASTMK010000000
VERSION     DASTMK010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286565"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFQ79_24375"
     CDS             <1..209
                     /locus_tag="VFQ79_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142880.1"
                     /translation="SPHDQYFFKRSGRMVSGEVTPPRLELANEDLVRAHLHAIWLAEA
                     GLTGRTSPRVDGSRCWSDIRPCVA"
     gene            complement(719..1300)
                     /locus_tag="VFQ79_24380"
     CDS             complement(719..1300)
                     /locus_tag="VFQ79_24380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142881.1"
                     /translation="METRSYRLDFSNGLSATAVWLKALTVTQDAPVVVVLHDQGRAAA
                     SEAVSNELNHGRLVFAVNLLFTGDATPKSPRPYDKYCQVVATLGDRPLGLQAAQLLAI
                     LEWIREEFRVGRLEVRSTGIRNQTRALVAAALRPELFSAVHIHEGVPSLRYLLETPVA
                     YLDAPELFCLDLYRHFDLDRLAALASPSNVVWH"
     gene            complement(1266..2642)
                     /locus_tag="VFQ79_24385"
     CDS             complement(1266..2642)
                     /locus_tag="VFQ79_24385"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0142882.1"
                     /translation="MTGKRIVGLLIAAAALAVAQTTAERVRAILQPPVHSAEVAAFQL
                     HQYLLAKIPLLPAPEDAKDWTAQARLLRKRLLRDIVFHGWPVEWVNSPPHFIETGVIE
                     TGKGYRIRKLRYEVVPGFSSTALLYEPESVSGKLPAILNLNGHVGRAGKTVEYKQKRS
                     INQALRGALALNLEWLGCGELDGPEQQHVFAGHLDLVGANGLGLFYLAMRRGLDYLAR
                     HAAVDMRRIGVTGLSGGGWQSILLGALDERVSVAVPVAGFMAMRSRLARPAEIGDNEQ
                     NATDLLTIADYSHLTALRAPRPTLLIYNAEDDCCFRAPLVKPHIFDPAIRFFRLYGQP
                     DSLAWHENTDPSDHNYQLDNRQQAYRFFARYLGLGGPESEFPVDGEVRSFDELAVGLP
                     PDNLSILGVARKLVERIERPLHRSQARKGIARADRALPAGSRNSGVRCRQHEEPRIGD
                     SLLSPGLQ"
     gene            complement(2639..2923)
                     /locus_tag="VFQ79_24390"
     CDS             complement(2639..2923)
                     /locus_tag="VFQ79_24390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142883.1"
                     /translation="MATGFSFEEAHATRFEVPAQGRHREGYREGSGPQPRHRVAHPGV
                     VDWFIPAGVQAGNLIGMFSETDLPFAVLENSVLEPGDAGADDDQIGALGR"
     gene            2987..6379
                     /locus_tag="VFQ79_24395"
     CDS             2987..6379
                     /locus_tag="VFQ79_24395"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0142884.1"
                     /translation="MPEFFDLIPSYGKETIMREYRYLPALFIFAALWLAFAGLKLNAA
                     ETTGTIAGVIVDSSGAVVPDVKIRITNESTGRQRETVSDGVGSFAFPLVDIGPYRATA
                     EKSGFRNFVQSGLVVRVNDRITLNIRMEVGSMEQQVTVTGQGSLVNTQTAEIRSLVDR
                     VRIEKLPLNGRNVLQLTLLTGGVLDSGGGTANQGFIHANAKIFPSASGGRADAMNFIF
                     DGGTNNDRYTNVAMPLPNPDAVQEFSFLTNSFSAEYGAASGGVVNVVSKTGTNELHGT
                     AYNYLRNSKLNATNFFTPGVSDRLKRNQAGFSVGGPVWLPRIYDGRNKTFFFLSFQET
                     FLRQSPVSANQRTLTADELKGDFSLQASLLRRPVLDPATGTDRSNRTPFPNNQVPIDR
                     LDPIMLKISTLLPVGDPATGFITSATARTMNNQPEWLARGDHNVSSANRLSFRYFRSL
                     FSPILSVEETNPYLTGNSGIRQFSQSFTLGDSHIFTPRLLANVNVTVSRIHNAETYMY
                     PGLANNNPAALGIMGFPNGSFFLRSPFVAVGYAGPYTTLASTNFQYQGSMTYIVGRHE
                     IKFGAQYMRSQLNQQRLALGGNWFFGTDYTDIPQSDFLMGLPNSFSAFGTFGEALRQN
                     RFDTYVQDNIRLSRRLSVSVGIRFDPFFPWAETNYDKVSLFRPGLKSQRFPNLPVGAV
                     VAGDPGVPEHGYPGDHNNFAPRVGFAFDPRGNGRTAIRGAAGFFFGQPGSASINQRSQ
                     VNFPFVTRADITSPGSLGPGAVANVFRTGTKDEIPNPFLTTIGVPPKEFPIPTPLLYT
                     STADNYGLPYTAQWNFSVEQQFGPDWTTTATYMGSKSTHLLMAYERNPSIYIPGNGPD
                     GRPLSTSANIDQRRPYGPAFTSVQELASDGNANYNALVLRVRKAVNGVHWWSRSTFEV
                     NHTWSHTLDVLSDVVSPGGSSPRNPFNARLDYGNSNFDHRHRFVASYVWALPPLTTMP
                     TAVRAVLGNWNTSSVMTFQDGNVFSLTGGLSQPFTTGDPATQAGADATLPSGRSKQER
                     ILRWFNTDAFRPTPVGAYGNTGRNILRGPGFANVDFSVYKNLPLPGDVEGRYIEFRSE
                     FFNLFNRVNLGMPNSNASNAFFGRILSAGSPRIVQFAVKLYF"
     gene            6405..7235
                     /locus_tag="VFQ79_24400"
     CDS             6405..7235
                     /locus_tag="VFQ79_24400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142885.1"
                     /translation="MISGLFICLASTILIEGQPGKANLVLRDSRAPGAVWNLRIPETV
                     HEDAGFVALSTPPSGVEWRRFGPQVGYELDVPRATPDLSYMARQKPPRTPALPGSYRI
                     AVRCKAEAESVRFAVRLTNTSGRDWRSAFMHLCLSHRDAPGFARSGAVWLKTALGLSA
                     GTEWKRSPGQYAAFALLLFGNSETRDPFYTDYIRAEGNARALGRYVFGCDNARPMCAG
                     IAAARPLFFMNNQANPCTDVAVAFGDLADGETGEGSGRVYIVPGGRTEFVRHVERDYP
                     "
     gene            7245..8630
                     /locus_tag="VFQ79_24405"
     CDS             7245..8630
                     /locus_tag="VFQ79_24405"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0142886.1"
                     /translation="MQNVNRRSFFVTLAAASTGRAASERYPLKWKAVTGIENNGVVAG
                     KIDGKVRLFVTGRMHYRNPPPLAGYLVSLDAAGHILYKDRAHTGGICVFPSFLEPKGV
                     GPSVFYACGPYQPDSVGEARLVNAHDGRTRWTQPTSNQFFGNGSCVLADVDGDREEEL
                     VYGDQAAVRCFRARDGKLKWFYNDRVVICHGRLAYGEINRDSRPELVFGTEYSNADKS
                     SSMVAINGRGKVLWRKDGIAGDMGSTPAVLADVDGDGRLEILKVELDLLGREKMPYAG
                     LFCYRGDGTLKYRADFGVSGMAVGDLDRDGSLEAVGISDGRDGGVNGRNEIRCLDLKT
                     GKMKWTTPVPRAWLGSQDPVMADLTGDGRLNVLVSSSNPSGYGRRKGEEPYGDIYIVD
                     GRGKIVWNTTLPDFVHQPFVSDIDGDGRNEIVLACHDGAIYCYGTPGKAGRLWSVTGG
                     SFERTYKAEPL"
     gene            8646..10760
                     /locus_tag="VFQ79_24410"
     CDS             8646..10760
                     /locus_tag="VFQ79_24410"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU0142887.1"
                     /translation="MQPWIVLAVLAASGTTGALHQKTLERLEETPALGGRTILIDRFH
                     VAVDWRPFGSVEEAAGAESDVDWFDTTRQGVARQAACTLSFAALELRRYLGRMGRRQW
                     ARIRVPGGEGDGLAITTLGRLTPEQLARMPAGIVEMLRGGPERFAVVPFEDGRRMTVM
                     LVGSDRVGALYAVYDFLERQGVRFIGLGESEEVVPQRADLLIPAAYSGKPGFGLRSLY
                     GYRKNRGTEPFFSWMAKNKLNAWGADDLLPGMRKRGFKLYGGNHWVQPEAGIRGNVCL
                     SDEPQTETFVRNVVNLLADGIYKDLDILEYLPADWGVRCEEDNKFGSPTDRDVLLVHR
                     LRKAIRAAYAAGRLRRDVQVFFYAYFDTVSPPTRPLPLDFDYQRTSVGFYAMRCFNHA
                     MNDPSCNEAFVAYIKRAGGEFAVSRPVNAQLNAYLRLWTRGETPWKGRVGYGDYYSMA
                     RLFQIPNPLMNIIAADIPYAHELGVWDARYMHPTTSDWGTKTLTNYQFAKMLWDPNLD
                     VPALLAEYFQLRYGAAAQPMCDFYFRLEGAMANIMWLKGDFVDRFNRIHKMKDTLFIW
                     DHLHLEPYQPAINDGVDLEESVTEMQRCRELLKRALALTRDEAAQRRIREDDAWFQYS
                     SNTIHFYYHMAKTYTRWVAGDRPGAVRSFAEAEKYATLLRTQMLPILVQGVHQPNQAD
                     GLEAARLMPAYEHFKKEFGGTQ"
     gene            10757..>11987
                     /locus_tag="VFQ79_24415"
     CDS             10757..>11987
                     /locus_tag="VFQ79_24415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142888.1"
                     /translation="MRTASWAMALLAAAFASGAETARPIRVVVPKGAAPRVEFGAARL
                     MDALRSVGYDPVRAAARGSGPAIVIGNPRKDPALAKLVRTASPALLNPEGFMLAGLGG
                     GVTAVGATGDTGLLYGCLELEARVLAARSVPPRLAVSDAPRFSLRGPAIGMQRPEITY
                     DGVQYDYLYTSENFPFFYDRAHWTRYLDFLLENRMNTLYLWTGHPFTSLLELPRYPEA
                     RELTPEQLRKNIETFTWLTSEADKRGIWVIQKFYNIHISHALAKARNVPIVYSRPTEL
                     SSAYIRYAVAEFIRSYKHVGFMLCLGEALQDDYDAEWLTGVILTGVNDGMRALGITEE
                     PPVIVRAHSTPIEQVMAAATPVYSNLLTMHKYNGEGLTTPEPRGEVRRLHESLAKLGS
                     AHIVNVHLLSNLEPFRWG"
BASE COUNT         2310 a         3745 c         3687 g         2245 t
ORIGIN      
        1 gaagtcctca tgatcagtat ttcttcaagc ggtccggacg aatggtgtcc ggtgaggtaa
       61 cgccaccgcg actggaactg gcaaacgagg atctcgttcg cgcgcacctg cacgcgatct
      121 ggctggctga agctggcctc accggacgca catcgccccg agtggatggt tcgcgatgct
      181 ggtctgatat acgtccctgt gtagcatgac ggtaatgttc acgagagcac tgaggagcaa
      241 gagcagatcg ccacgctggt caacgaggcg ctcggcttgc gcctgcgttg tgcgaatcgg
      301 agtgcccgcc gcttgccagg gacgacatcc tcgtggtggc accgtacaat ctccaggtcc
      361 gtagactcga tcgtcggctc aagagcgagc acctgtcgtc aacttctcga tgtgttcatc
      421 aagtgggagc gcgccgccga acggcatcgt tcctcttcag ccgactcgcc tgaatgtcgc
      481 tatctccttg cagagaccat ggccaggctg agtgacaatg tgccatggcg cccaccacct
      541 ggcgtttctt tggatgtctc tgcttgtcgt gtggtgactt ccggaattgg gcgagatgtc
      601 ggctgacgct gcactctgtt catcgggttc acgaaccgtt tttcccttac gtgaggactc
      661 ctgacacgaa ttcctgcgtt actcagcagc gaatcatccg gcttcataat gaacaaccct
      721 aatgccagac gacgttgctg ggcgatgcta gcgcggctag gcggtccagg tcgaagtgcc
      781 tgtagaggtc caggcagaac aactcgggcg catcgaggta tgcgacgggg gtttcgagca
      841 ggtaccgcaa ggacggcacg ccctcgtgta tgtgcaccgc ggaaaacagt tccggccgga
      901 gggcggcggc gaccaaagcg cgcgtctgat tgcggattcc ggtacttcgc acttcaagtc
      961 tgccgacacg gaattcctcg cgaatccact ctaggatcgc caggagctga gcggcctgaa
     1021 gccccagcgg acggtcgccg agcgtggcga ctacctgaca gtatttgtcg tagggccggg
     1081 ggcttttcgg agtggcatcc ccggtgaaga gaagattgac cgcaaaaacc agccgaccat
     1141 ggttcagttc attcgagacg gcctcggaag cggccgcgcg tccctgatcg tgcagcacga
     1201 ccacgacggg cgcatcttgc gtcacagtga gggctttcag ccacacggcg gtagcactga
     1261 ggccgttact gaagtccagg cgataggagc gagtctccaa tcctcggctc ttcgtgctgg
     1321 cgacatcgaa cgcctgagtt acgcgaaccg gctggtagcg cacggtctgc gcgagcaatt
     1381 ccctttcgcg cttgggacct gtgcagcggc cgctcaatcc gctcgaccag cttcctcgcg
     1441 acccccagga tcgagaggtt gtccggaggc agcccgacag ccagttcgtc gaagctgcgc
     1501 acttcaccgt ccaccgggaa ctccgactcg ggccctccga ggcccaagta gcgtgcgaag
     1561 aaccggtagg cctgctggcg gttatcgagc tggtagttgt ggtcggaagg atccgtattc
     1621 tcgtgccatg cgagggagtc gggctgaccg taaagacgga agaaccgtat tgcggggtcg
     1681 aagatgtgcg gcttcacgag cggcgcgcgg aagcagcagt catcttcagc attgtatatg
     1741 agcagcgtgg ggcgcggcgc ccgcaatgcc gtgaggtgag aataatcggc gatcgtgaga
     1801 aggtcggtcg cgttttgttc gttgtccccg atctctgcgg gccgtgccag ccgggaacgc
     1861 atggccatga aaccagccac tggcacagcc acactcacgc gctcatccag tgcaccgagc
     1921 aggatactct gccagccgcc gcccgaaagc ccggtgacgc cgatacgccg catgtcaact
     1981 gccgcgtgcc gtgcgaggta atcaaggccc cggcgcatgg caagatagaa caaaccgagg
     2041 ccgttagcgc ctaccaggtc caggtgacca gcgaagacgt gctgctgttc cggcccatcc
     2101 agttcgccgc agcccagcca ctcgaggttc agcgccaaag cgccgcgcaa cgcctggttg
     2161 atcgagcgct tctgtttgta ctccacggtt tttccagccc tgcccacgtg gccgttcagg
     2221 ttcaggatgg ccggcaactt gcccgatacg ctctcgggtt catagagaag cgcggtggaa
     2281 gagaagcccg ggacgacctc atagcgcagc ttgcgaattc ggtatccctt gcccgtctca
     2341 atgacacccg tttctatgaa atgcggcggc gaattcaccc actccaccgg ccagccgtgg
     2401 aaaacaatgt cgcgcagcag gcgcttccgc agaagccgcg cctgcgcggt ccaatcctta
     2461 gcgtcctcag gcgccggtaa caagggtatc ttggcgagta agtattggtg cagttgaaag
     2521 gccgcgacct cggcggaatg cacgggcggc tggagaatag cgcgcactct ctccgcagtc
     2581 gtctgcgcga ccgccaaagc cgccgcggcg atgaggaggc cgacgatgcg ctttcccgtc
     2641 atctgccaag ggccccgatc tggtcgtcgt cggcccccgc gtcgccgggc tcgagaacgg
     2701 aattctccag cacggcaaag ggcagatccg tttccgagaa catgccgatc aggttcccag
     2761 cctgaacgcc ggccggaatg aaccagtcca ccactcccgg atgcgcgaca cggtggcgag
     2821 gctgaggtcc gcttccttcg cgatatccct cacggtgacg cccttgcgcg ggcacttcga
     2881 atctggtcgc atgcgcctct tcaaaactaa atcctgttgc cattcgagtc aagtattccg
     2941 gggatttcac gccgatacga agaatcttcg tcactcccgg acagcgatgc ctgaattttt
     3001 cgatctcatt ccgtcctatg gcaaggagac cattatgcgc gaatatcgct acctgcccgc
     3061 actgtttatc tttgcagccc tgtggctcgc ctttgcgggg ttgaaactca atgcagcgga
     3121 aaccaccggg accatcgcgg gggtgatcgt cgactcctct ggcgccgtgg tccccgacgt
     3181 gaagatccgc atcaccaacg agagcacggg ccggcagcgg gagacggtga gcgatggcgt
     3241 gggcagcttc gcctttcctc tagtcgatat cggcccctat agagccaccg cggagaagtc
     3301 cggcttccgc aactttgtgc aaagcgggct tgtagtccgg gtgaacgacc gcatcacgct
     3361 gaacatccgg atggaagtcg gctcgatgga acagcaggta acggtgacgg gacaaggctc
     3421 actcgtcaat acccagacgg ctgaaatccg ttcgttggtg gaccgcgtgc gtatcgaaaa
     3481 gctgcccctg aacggccgta acgttttgca gttgacgctg ctgacgggcg gtgtgctcga
     3541 ctcgggtggc ggcacggcga accagggctt tatccacgcc aatgccaaaa ttttcccctc
     3601 ggccagcggc ggccgggccg acgccatgaa cttcatcttt gacgggggca cgaacaacga
     3661 ccgctacacc aatgtggcga tgcccttgcc gaaccccgac gcggtgcagg aattcagctt
     3721 tctcacgaat agcttcagcg cggagtacgg agcggcttcg gggggcgtgg tgaacgttgt
     3781 gagcaagacg ggcaccaacg aattgcacgg cacggcgtac aattacctgc ggaactccaa
     3841 gctgaacgca accaacttct tcacgcccgg agtctccgac cggcttaagc gcaaccaggc
     3901 cggcttcagc gtgggcggtc cagtgtggct gccacggatt tacgacggcc ggaacaagac
     3961 gttttttttc ctctcgttcc aggagacgtt cttgcgtcag tcgccggtaa gcgctaatca
     4021 gcgcactctc acggccgatg aactgaaagg cgatttctct ctgcaggcgt cgcttctcag
     4081 gcgaccggtg ctggaccccg ccactggaac cgacagatcc aatcgcactc ccttccccaa
     4141 caatcaagtg ccgatagacc ggctcgaccc gatcatgctg aagatcagca cgctactccc
     4201 tgtcggcgat ccggctacag gattcattac ctccgcgacg gcacgcacca tgaacaacca
     4261 gccggagtgg ctggcgcgcg gggaccacaa cgtctcttcg gccaaccggc tctcgttccg
     4321 gtacttccgc agcctgttca gccctatcct gtccgtcgag gaaaccaatc cttacctcac
     4381 gggtaatagc gggatcaggc agttctccca aagtttcact ctcggcgaca gccacatttt
     4441 cacaccgcgc ctgctggcga atgtaaacgt gaccgtgagt cgtatccaca acgccgaaac
     4501 ctacatgtac cccggcctgg cgaacaataa tcctgcggcg ctcggcatca tgggattccc
     4561 gaacggctct tttttcctgc gttcgccgtt tgtggcggtg ggctacgccg gaccgtacac
     4621 cacgctggcc tcgaccaact tccaatacca gggcagcatg acctacattg tggggagaca
     4681 cgagatcaag tttggcgcac agtatatgcg cagccagttg aaccagcaac ggcttgcgct
     4741 cggcggcaac tggttcttcg ggaccgacta caccgacatt ccacaatccg acttcctgat
     4801 ggggctgcct aattcgtttt cggcgttcgg cactttcggc gaggcgctcc gccagaaccg
     4861 ctttgacacg tacgtacagg acaatatcag gctctcccgc cggctcagcg tcagcgtggg
     4921 aatccgcttc gacccgttct tcccttgggc cgagacgaat tacgacaagg tatcgctgtt
     4981 ccgcccggga ctgaagtcgc agcgcttccc gaacctgcca gtgggcgccg tggtcgcggg
     5041 tgaccccggg gttccggagc acggctaccc gggagaccat aacaacttcg ccccgcgcgt
     5101 cggtttcgct ttcgacccgc gcggcaacgg aaggactgca atccgcgggg ccgccggctt
     5161 cttcttcggg cagcccggat cggcctccat caatcagcga tcgcaggtga acttcccatt
     5221 cgtcacacgg gccgacatca cctctcccgg ttctctcggt cccggagctg tggcgaatgt
     5281 cttccgtacc ggaacgaagg acgagattcc gaacccattc ctcacgacca ttggggtgcc
     5341 gcccaaggag ttcccgattc cgacacccct tctgtatacc tccaccgcgg ataactacgg
     5401 gctgccgtac acggcgcagt ggaacttcag cgtggaacag cagttcggcc ccgactggac
     5461 caccacagcc acctacatgg ggtcgaaatc gacacacctg ctgatggcgt acgaacgaaa
     5521 cccgtccatc tacattcccg gaaacggacc ggacggcaga ccgctttcga cctcggccaa
     5581 tatcgatcag aggcgcccct acggcccggc gtttacgtcg gtgcaagagt tggccagcga
     5641 tggaaacgcg aactacaacg cgttggtgct gcgggttcgc aaggccgtga acggggtgca
     5701 ctggtggtca cgctcgacct tcgaggtgaa tcacacctgg tcgcatacgc tggacgtcct
     5761 ctccgacgtg gtcagtccag gcggctcgag tccccggaac ccattcaacg cacggctgga
     5821 ttatggaaac tcgaacttcg atcaccgcca tcgcttcgtg gcctcctacg tctgggccct
     5881 gccgccgctg actaccatgc cgactgcagt ccgggctgtg ctggggaact ggaatacgtc
     5941 ttcggtgatg actttccagg acggcaacgt ttttagcctc acgggcggcc tctcgcaacc
     6001 attcacgacg ggcgaccccg ccactcaggc gggcgctgat gcgactctcc cgtcgggccg
     6061 ttcgaagcag gagcgaattc tccggtggtt caacaccgac gcattccggc ccacgcccgt
     6121 cggcgcgtac gggaatacgg ggaggaacat cctgcgcggt cccggcttcg cgaacgtaga
     6181 cttttcggtg tacaagaacc tcccgctacc gggagacgtc gaaggacgct acattgagtt
     6241 ccgttccgag ttcttcaacc tgttcaaccg cgtcaatctc gggatgccga attcgaacgc
     6301 gagcaacgcc ttcttcggcc ggatcctgtc ggccgggtcg ccgaggatcg tgcagttcgc
     6361 ggtgaaattg tatttttgag gacgagcgac ctcctagttg agccatgata tcgggtctgt
     6421 ttatctgtct tgcctcgaca atcttgatcg agggccagcc ggggaaggcg aacctggtgt
     6481 tgcgggattc gcgtgcgcct ggggcggtct ggaatttgcg gatcccggag acagtacatg
     6541 aggatgcagg cttcgtggcc ttgagcacgc cgccttcggg agtcgaatgg cgtcggttcg
     6601 gaccgcaagt cggctacgaa ctggatgtgc cgcgagcgac acccgacctg tcctacatgg
     6661 cgcggcagaa gccgccccga actccggcgc tgccagggtc gtaccgcatc gcggtgcgat
     6721 gcaaggcgga ggccgaaagc gtgcgcttcg ccgtccggtt gaccaacacg tccggccgcg
     6781 actggcgcag cgccttcatg catctctgtc tctcgcaccg cgatgccccc ggcttcgcgc
     6841 gaagcggtgc ggtttggctc aagaccgcgc tggggctttc cgccgggacg gaatggaaac
     6901 gttcgcccgg acagtacgcc gccttcgcgt tgctgctgtt tgggaattcg gagacccgtg
     6961 atccctttta caccgattac atccgcgcgg aaggtaacgc ccgcgcactc ggacggtacg
     7021 tattcgggtg cgacaacgcc cgcccaatgt gcgccggcat cgccgccgcg cgcccgctct
     7081 tcttcatgaa caaccaggcc aacccgtgca ccgacgtcgc cgtcgcgttc ggcgatctcg
     7141 cggacggtga aaccggcgaa ggctccggcc gggtatacat cgtgccgggc ggtcgcacgg
     7201 agttcgtccg gcacgtggaa cgggactacc cttaggacaa atccatgcaa aacgtgaatc
     7261 ggagatcttt ctttgtcacg cttgccgctg cttctacggg gcgggcagcg tcggagcggt
     7321 atcccttgaa gtggaaggcg gttaccggaa tcgagaacaa tggtgtggtc gcgggcaaga
     7381 tagatggcaa ggtgaggctg ttcgtcaccg gccgcatgca ctaccgcaat ccaccgcccc
     7441 tggccggtta cctggtttcc ctggacgcag ccggccatat cttgtacaag gaccgcgcac
     7501 acaccggtgg catctgcgtt tttccgagct tcctggaacc gaagggagtg ggcccgtccg
     7561 tcttctacgc gtgcgggccc taccaaccgg attctgtggg ggaggcccgg ctggtaaacg
     7621 cgcacgacgg ccggacccga tggacgcagc ccaccagcaa ccagttcttc ggcaacggct
     7681 cgtgcgtact ggcggacgtg gacggcgacc gggaggaaga actggtctac ggcgaccagg
     7741 cggcggtgcg ctgcttccgt gcccgagacg gcaagttaaa gtggttctac aacgaccgcg
     7801 tggtgatctg ccacgggcgt ctcgcctacg gcgaaatcaa ccgggactcc cgcccggaac
     7861 tggtcttcgg caccgaatat tcgaatgccg acaagtcgtc gagcatggtc gccattaacg
     7921 gccggggcaa ggtgctctgg cggaaggacg gcatcgcggg cgacatgggt tccacgcccg
     7981 ccgtgctggc cgacgtggac ggcgacgggc gtctcgagat cttgaaagtc gaactcgatt
     8041 tattggggcg cgagaagatg ccctatgcgg gactattctg ctaccgcggc gacgggacat
     8101 tgaagtaccg cgcggatttc ggtgtcagcg gaatggctgt gggcgacctg gacagggacg
     8161 gctccctgga agctgtcggc atttccgatg gccgcgatgg cggcgtcaac ggcaggaatg
     8221 agattcgctg cctcgatctc aagactggaa agatgaagtg gacaacgccc gtcccacgcg
     8281 cttggctagg cagccaggat cccgtcatgg ccgatctcac cggcgacggg cgcctcaatg
     8341 tactcgtgag ttccagcaat cccagcggat acggacggcg caagggcgag gagccgtatg
     8401 gcgacatcta catcgtcgac ggccgcggca agatcgtttg gaacacgacc ctgccagatt
     8461 ttgtgcacca accgttcgtg agcgacatag acggcgacgg ccggaacgaa atcgtactgg
     8521 cgtgccatga cggcgcgatc tactgttacg gaacgccggg caaggcaggc aggctctggt
     8581 cggtaacggg tggtagtttc gagcgtacct acaaggcaga gccgctctga gctcggggag
     8641 gaacgatgca gccatggatc gtgctcgccg tgctggccgc gagtggtacc accggggcac
     8701 tgcaccagaa aacgttggag aggctggagg aaacgcccgc gctgggcggc aggacgatcc
     8761 tcatcgaccg cttccatgtt gccgtggact ggcggccgtt cgggtcggtt gaggaagcag
     8821 ccggggcgga atctgatgtc gactggttcg acacgacgcg tcagggggtg gcacgccagg
     8881 cagcctgcac cctcagcttt gccgcgctgg aattgagacg gtacctcggc cgtatgggtc
     8941 ggaggcagtg ggcccggatc cgcgtgccgg gcggtgaggg cgacggactc gcgatcacca
     9001 ccctcgggag gctaacgcca gagcagctcg cccgcatgcc ggctggtatc gtagaaatgc
     9061 tgcgtggagg ccccgagcgc ttcgctgtgg taccgttcga agacggccgt cgcatgaccg
     9121 tgatgctggt gggctcggac cgcgtcggcg cactgtacgc cgtctacgac tttctggaac
     9181 gccagggagt gcggttcatc ggcctcggtg aaagcgagga ggtggtgcca caaagggcgg
     9241 acctgctcat ccccgcggcg tactcaggca aacctggatt tggtctgcga tcgctttacg
     9301 gttatcggaa gaaccgcggc accgagccct ttttcagttg gatggcgaag aacaagctga
     9361 atgcctgggg cgcggacgat ctcctgccgg gaatgcgcaa gcggggtttc aagttatacg
     9421 gcggcaacca ctgggtgcaa cccgaagccg gcattcgagg caacgtctgc ctgagtgacg
     9481 agccgcagac cgaaacgttc gtacggaatg tagtcaacct cctggcggac ggaatctaca
     9541 aagacctcga tatcctggaa taccttcctg ctgactgggg cgtgcgttgc gaggaagaca
     9601 acaagttcgg ctcgcccacg gaccgcgacg tccttctcgt gcaccggctg cggaaggcca
     9661 tccgcgcagc ctacgcagcg ggcaggctga ggcgcgacgt gcaggttttc ttctacgcgt
     9721 acttcgacac ggtcagcccg cctaccaggc ccctaccgct ggacttcgat taccagcgta
     9781 ctagcgtggg cttctacgcg atgcgctgct tcaaccatgc catgaacgac ccatcctgca
     9841 acgaggcctt cgtcgcgtac atcaagcgcg cgggcgggga atttgccgtt tcgcggcctg
     9901 tcaacgccca gctcaatgct tacctgagac tttggacgcg gggggagacg ccgtggaaag
     9961 gccgtgtcgg gtacggtgat tactacagca tggcacggct cttccagatc cccaacccac
    10021 tcatgaatat catcgccgcg gacattcctt atgcccacga gctgggcgtc tgggacgctc
    10081 gatacatgca cccgaccacg agcgactggg gcaccaagac gctcaccaat taccagttcg
    10141 ccaagatgct atgggacccg aacctggacg tgcctgcctt gctcgcggag tacttccagc
    10201 tgcgctacgg tgccgccgca cagcccatgt gcgacttcta cttccggctg gaaggggcca
    10261 tggcgaatat catgtggctc aagggcgact ttgtggatcg tttcaaccgc atccataaga
    10321 tgaaagacac gttattcatc tgggatcacc tgcatctcga gccctaccaa ccggccatca
    10381 acgacggcgt cgatctggag gaaagcgtga cggagatgca gcgctgccgc gagctcctga
    10441 aacgggcgct ggcgttgaca cgggacgagg cagcgcagcg ccgcatccgc gaagacgatg
    10501 cctggttcca atactcgtcc aacaccatcc acttctacta ccacatggcg aaaacctaca
    10561 ctcgctgggt ggccggcgac cgccccggcg ctgtgcggag tttcgcggaa gccgaaaaat
    10621 acgccacgct actgcgcaca caaatgctgc cgatcctcgt ccagggcgtg caccagccga
    10681 accaggcgga cggtcttgag gcggcgcgtc tgatgccggc ttacgagcat ttcaagaaag
    10741 agtttggagg cacgcaatga gaacggccag ttgggcgatg gccctgctgg ccgcagcgtt
    10801 tgcgtccggt gcggagacgg cgcgcccaat acgcgtggta gttccaaaag gcgccgctcc
    10861 acgcgtcgaa ttcggcgcgg ccagattgat ggacgctctt cggtcggtag gctacgatcc
    10921 ggtgcgcgcc gccgcgaggg gcagcggccc cgctatcgtg attggcaacc cgaggaaaga
    10981 ccctgcgctg gcgaagttgg tgcggaccgc gtcgccggcg cttctaaatc ccgagggttt
    11041 catgttggca ggcctcggag gcggcgtcac cgcggtaggc gcaaccggcg atacgggcct
    11101 cctctacggc tgcctggaac ttgaggctcg ggtgctggcc gcccgctccg tgcccccgag
    11161 gctggctgtc tccgatgcgc cgcgcttttc cctgcgcggt ccggccattg ggatgcagcg
    11221 acccgaaatt acctacgatg gcgtccagta cgattatctc tacacctctg agaacttccc
    11281 tttcttctac gaccgcgcgc actggacgcg ctacctggat ttcctgcttg agaaccggat
    11341 gaacacgctg tacctgtgga cgggccaccc gttcacgtcg ctgctagagc tgccacgtta
    11401 cccggaggcg cgcgaactca cccccgaaca attgcgtaaa aacatcgaga ccttcacgtg
    11461 gctcacctcg gaggcggaca agcgcggcat ctgggtcatt cagaagttct ataacatcca
    11521 catctcgcat gccctggcca aggcgaggaa cgtgcccatc gtgtacagcc gccctaccga
    11581 actgtcgtcc gcctacatac gctacgctgt cgcagagttc atccgcagct acaagcatgt
    11641 agggttcatg ctctgcctcg gcgaagccct ccaggacgat tacgacgccg agtggctgac
    11701 cggcgtgatc ctcaccgggg tgaacgacgg catgcgggcg ctcggcatca cggaagaacc
    11761 gcccgtcatc gtgcgggcgc acagcacgcc catcgagcag gtgatggcgg cggcgacgcc
    11821 ggtctattcg aacctcctga cgatgcacaa gtacaatggc gaaggcctca ctacgccgga
    11881 acctcgcggc gaggtccggc gcctccacga atcgctggcg aaattggggt cggcgcacat
    11941 cgtcaacgtg cacctgctgt ccaacctgga gccgttccgc tggggtg
//
LOCUS       DASTMK010000594        15698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_93296, whole genome shotgun sequence.
ACCESSION   DASTMK010000594 DASTMK010000000
VERSION     DASTMK010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15698
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93296"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VFQ79_24420"
     CDS             complement(<1..1492)
                     /locus_tag="VFQ79_24420"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0142889.1"
                     /translation="MPQPARLCAIVLLAAIVVSGQTAGGSLSGTVFDQSAAAVPGATV
                     KLAQQPVGIERTVSTNANGYYTFQALPRGEYTIEIEQSGFATIRRSIALQIQENATLD
                     IELAVGDISTHISVSAEAPVLQTNDAIVGQVINGQQTVELPLNGRQFSQLILLSPGAA
                     PRGGGQQAGFTVQLGGGGISPAINGQSGNQNNFTLDGLDNNSRFANIWAISPPPDAIQ
                     EFKVESGQRAGANVNIATRAGTNEFHGSVWEFLRNDKLDARNTFDTRKPPYRQNQYGV
                     YAGGPLFLPKIIDGRKTNSWISGYWEGFRSRRGQTFFATVPTAEMISGDLSRFLGSQT
                     GNVRAGQIYDPFSTTPDPLNPGTYLRQPYPGNIVPSSRISSVATDYLKYFYPTPNQPG
                     FPNNFVSVQSREINSDQGGIRFDQRVGRENSLFVRANVFNGQSVSPSSVPLNAQYLEN
                     RTRSIGGSYTHVFNPTLIGTLGVAYTRDFTPNHTPGVPKDLFDRLGL"
     gene            complement(1606..2460)
                     /locus_tag="VFQ79_24425"
     CDS             complement(1606..2460)
                     /locus_tag="VFQ79_24425"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0142890.1"
                     /translation="MDTLPVSRRTLSRTRTVRVGVCQILVIDGDREGNFRRIEYALKS
                     ACTEGANIVVFPESSILGWENPDAHRLATPLPGIDSERISDLAEKYDVMIGIGLDEKD
                     NGCLYDSAILVSRKGRLLWKHRKLNVLPELMEPPYSKGSMDGIGAVDTEFGRIGMLVC
                     ADTFRDEYAEVMSRARPDLVLVPYGWAARTDKWPGHAQELEQLVRRRAMGWKCPVAAA
                     DCVGVMMHGPWKGWTFGGASLVVDASGSVLALLRDRDVDVRVISVEPGNGNAGGCRDG
                     ATVASDNC"
     gene            2667..3809
                     /locus_tag="VFQ79_24430"
     CDS             2667..3809
                     /locus_tag="VFQ79_24430"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HEU0142891.1"
                     /translation="MYDARLAYDVKVMIGIGAWIHEHGNWSVYIKEDALKDQHLPDLA
                     SWDGDGIIANFDDRAIFSAVTNSRLPSVAFGGGGSCDQSRCKVPYFFGDDEAIARLAA
                     DHLLSRGLRSFAYCGYPRDAINGWSERRAHAFCARVAQRGFKCEVYRSSQRTEQEWVA
                     LRRSISSWLTTLPRPLGLMAANDRRGRQVLEACRALGLRVPDEVAVIGVDNDELFCQM
                     SSPPLSSVEHGAKELGYRAAALLEEMMTGRKAERRRYVIPPVGVVTRQSTNVLAVQEP
                     KVREAMSFIADTACHGIKVRNVAEIVGLSRSALEKRFKATLGYTVPQAIRRVQLERAR
                     LLIVETKLPLKQVADNTGFRSVQHLTTVFGNTFGQPPARYRKSGHV"
     gene            complement(3886..3962)
                     /locus_tag="VFQ79_24435"
     tRNA            complement(3886..3962)
                     /locus_tag="VFQ79_24435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3926..3928),aa:Arg,seq:tcg)
     gene            4183..4419
                     /locus_tag="VFQ79_24440"
     CDS             4183..4419
                     /locus_tag="VFQ79_24440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142892.1"
                     /translation="MTQSFRLYASALHPRQWIAWSDATGWVQFPNEDNGWELRKSARG
                     LDPVHLRATPLREAANTGIPTGPVSFGSQPRRAA"
     gene            complement(4556..5401)
                     /locus_tag="VFQ79_24445"
     CDS             complement(4556..5401)
                     /locus_tag="VFQ79_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0142893.1"
                     /translation="MDRRVFLQTVGAGSGAALAGKAAQMKPVERKGRIKQSACRWCYS
                     KIPAEDFVREGARLGLKSIDLAPAAEWPVLKKYGLPPTMIGGGTTIPDGFNRKENHAG
                     IEERFRESVRNAVTVGAPSIIVFSGNRRGMSDEEGLENCVTGLKKIMPIAEDKGILVC
                     MELLNSKVDHADYMCDHTKWGVALAKRVASPYFKLLYDIYHMQIMEGDVIRTLRENAE
                     YIGHLHTGGNPGRNEIDGTQELNYRAIANAVVEMGFSGYMAHEFIPKRNPLESLAEAV
                     ELCDV"
     gene            5509..7191
                     /locus_tag="VFQ79_24450"
     CDS             5509..7191
                     /locus_tag="VFQ79_24450"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0142894.1"
                     /translation="MKSKFYSSLFLLTGLSLTPPLGAQSADLILYNGKIFTSDPSRPF
                     TQSVAVRQNRFLAVGTDQDVERYANANTRRIDLKQKLVIPGINDSHTHFAPMPPLVNL
                     GLWPDGAPPPDPTFDQVKAAIVAALSAQNPPAGTWLYADVGGRVFADPNATRLALDQV
                     APNHPIELRGWAGHGSIINSAAIRALRLSERESDPAGGWYERDAGGNLTGVLHGMASF
                     NLRSCLAAAVDENVGAAQIRAMAQQAAALGITSIQNMSGLPPNKMVRILNRAGLPIRW
                     REIPVPGTMQKCPALDVPPLPIVIPQGNVTLSGVKYILDGTPVERMAFMYERYLDVPG
                     SFFGKLYFTPGDIDRRLALNALSFPQPLFHVVGEATADLLLDLAERNPLSVLLRLVRP
                     RIEHGDFLGTDDYQALRRLGAIVVQNPTHFNIVDVFMQRYGPVKTAHAQPLKSLLRER
                     IPIALGSDGFLHPYINLLLATVHPINPAEAITREEALRAYTSGSAYAEFAEREKGTIA
                     AGKLADLAVLSDDVLTLPLPPPPPAPPLQWFGVHSVLTMVGGTIVHQTPGLN"
     gene            7250..9856
                     /locus_tag="VFQ79_24455"
     CDS             7250..9856
                     /locus_tag="VFQ79_24455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142895.1"
                     /translation="MIKRRDFLYLAPALCGMQAGTDAAQMEAGAEPISEPHFPSRLHQ
                     FVWRNWELANIERMASVVRGTPAGLLQIGASMGLPEKRKLTEDQLARIYITVIRQNWH
                     LLPEDQIVGLLGWDRQKYEFTLKEDDFLDVKLGRKKPKCEPLVYAEPSAQEKARAAEI
                     RNTVRRTFGKALDEPGEDLFAFIADLSDTGFRTLRAPGTKPAADEIDLTNGWSIAAGG
                     AFSRQAARFREYLRSSMNAVVAENAQKKIVLEVRPPEAAGRESFQIEVGDNQLVLTGT
                     SHAAVVEGLYLLQDRMESREGPFLKKGSIHRNVEWNPRYLYSYFALYGDPLMEPERDP
                     FPDGYLEKLARAGINGVWMQAVLNTLAPSKRFPEFGEGWQTRLKNLNALVQRARQFGV
                     GIFLYLNEPRAMPPAFFEKYPDVRGSSHQGLNAMCTSVPAVRDWLRESLAHVFANVPD
                     LGGIFTITMSENHTNCFSHGGAWGTGTPNAGDCPRCSKRTGHDTIAELLETFREGVRS
                     HNARADVIAYDWGWGYALAEKLIPLLSKEVKVLSISEWEAPVDRGGVQTRVGEYSISV
                     AGPGPRATRYWNIAKQNGNPTMAKLQFNNTWEISAVPYIPVTHLILEHCENLTRAGIN
                     GIMPSWTCGGYASPNLAAAKSYYYSPRPEQPEILRAVAVQRFGSSAADAIVKAWHQFS
                     EAFREFPYGVHVYIIPTQHGPANPLRLTPTGYRPGMILFPYDDYKAWSAKYPPEVVQR
                     QFAKMASLWNAGLRLFEKAIPEVSPAKKRNAELDLAIARTCAHHFESTANQVEFYMLR
                     DKSDPAALRRMRQLAEQEIDLARRQYPIARKHSVIAYEASNHYYYTPLDLVEKVLNCR
                     HVISEIDRRNSA"
     gene            9875..10873
                     /locus_tag="VFQ79_24460"
     CDS             9875..10873
                     /locus_tag="VFQ79_24460"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142896.1"
                     /translation="MRIGLVGCGLFGESHLMGFRAVPGAEVAAVYDVDSARAREMARV
                     FSIPRVCDSLDELCSLPELEAVDVVTPEELHLEPVLKAIARGKHVFVEKPLATDLEHC
                     SQMIRAANDAGRILMVGQLVRFETKAAMLKDEVASGKLGNVISMHARRNRPKALLERY
                     GRTHPVVENSIHDIDLMLWYTGRPVRRVRGYDRKAIATRHADTFWGIIEFEGGPIGVV
                     ETIWLLPKPAGVLLDDAFQLIGDQGIANMALFPGSLSFWRDTGFEIPDIGYDPRVHGA
                     ALGALRDELAYFCDCVRGGRMPEIITAVEAKNAVRVAMALMESAHLDQDVEIKSWN"
     gene            10897..12039
                     /locus_tag="VFQ79_24465"
     CDS             10897..12039
                     /locus_tag="VFQ79_24465"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0142897.1"
                     /translation="MKSQFKALFPRAVETVYLDTAAEGLPAPGLAESFAEYCRDKSLG
                     TPGRRRFHRVEAETTSLCARLLDTSPENVTLLSNASEAFNVLAGSLEWKAGDRVIISD
                     LEFPSNVYPWLRLEQVGVEVVVVSGDGGALRFEDIERHINGSTRLISLSLVSYRTGAF
                     LDCVPRLSAEAHRVGAFLSIDATQALGRLPLNVEGIDYLMSSSFKWLLGPHGLGVVYV
                     SPELRERLNPRAVGWYSVRNCFTPDRFERYELKSGAACLSSGMPNFPSLYALRNSLQF
                     VLRTGIDSIRDRLLEPVALLRRRLAAAGLSLLTPEDPRLASGIVAFEHPHAAQIGSAL
                     EEEGVIVWAGDGRVRASLHLYNDEEDVDRYVSILDGVLKKEKVAHG"
     gene            12032..13042
                     /locus_tag="VFQ79_24470"
     CDS             12032..13042
                     /locus_tag="VFQ79_24470"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEU0142898.1"
                     /translation="MGEMAWGGLAAVVFGGVLQGAFMLPMKWTRRWAWENTWLIFAST
                     AYLLCPWLLVLTTIPQAFDIYRESSAAALAIVLLFGLGWGAGAVTFGLGVDAIGLALG
                     FAIILGIAAAVGALVPLWMLPVSPSAAKLAVTLAALAIMLVGVAVCSLAGKWKDKPAE
                     GNRRSSYQKGVLICLVSGLLSACGNLGFAFGQPIIDRAQASGVPPYLASNIVWALLTI
                     ALFLCNAGYALLLLRRNGTAKQFRLPGTGKYFAFGCLMGVMWMGGYMFYGAGARRLGE
                     LGPSLGWVILMSGMVVTANVLGVLTGEWAGAPRWATRKLVWGIALLLAAIVVLGYANS
                     LA"
     gene            13142..13333
                     /locus_tag="VFQ79_24475"
     CDS             13142..13333
                     /locus_tag="VFQ79_24475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142899.1"
                     /translation="METQTLEFTPEQLALIVELLENERRTLSLQTRHSFSHTYRATLQ
                     TKLTMVDELLNQIRQHQPA"
     gene            13424..>15698
                     /locus_tag="VFQ79_24480"
     CDS             13424..>15698
                     /locus_tag="VFQ79_24480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142900.1"
                     /translation="MFYSGEHWRSVPNSEKPRLLKRITNGHRYPALKDPFVWVEGEAA
                     QPGELPLNQQLHVRDILRREPPLSFYPGWSLYALDLDIAQDLGQQAQERYEWLRVFFL
                     LRDEDGAGGEASAVLLDGRGPAIHEFNKRIGLELTPDTALEYLDFFTASVYGDLGPFL
                     ILSAGEDLIDVSARNPPAEAFLQRFGAKSSEVFAKAEPQRGEGSGTTIAAARGTIRPP
                     YQVSLQQEDQPEGDRQTFAVAGVSMQYGGGLFDSALEVARDRSYLGGIVMLGDNLVAN
                     DIPIVSYEVVRQPLLSIPCLARRRRNFGYKTMLPQAFASAGREAFARSHVFVPGDLDL
                     RGRTFKNAVKLDNWMIEGSLLLDRATFEDDLILQHCSILGGFSARRMVCQGRFRVLST
                     DVLGNSSRRDAPKGMVLRAAVLSRPARLKRVRVAGELVLREAECKSKLEIFGLTTRPS
                     TPYPNEKTVPGDVDAIGCKVQRSFLMKPAKQQSLAGETEAPNDLQGRFNADHCEVGDE
                     LELAGCEIAGGLTFTDAQVSGQVTLAGVVSKGGEDGRAIDLSGARLAGGVWIGSHRAE
                     GKNTRTISGTVTLRGSQIGWLACTDVEVRGSLDLTLMTCPQPVEIDVCSISGSLQLSG
                     ISAHSEVRILGVHIGGRIEIFSGEIGSLQIRPGVSYTMEASRWKLTGFHDLYAQDLEV
                     RGDFSCWGTECTDDFVLDSCTVSGRCSFWRDPWPRILSWNHLPVARWKEPKPNALRVC
                     TSIAGKLDLQTLSATSID"
BASE COUNT         3049 a         4623 c         4689 g         3337 t
ORIGIN      
        1 agaggcctaa ccggtcgaac agatccttcg gcacaccggg ggtatggttc ggtgtgaagt
       61 ctcgcgtgta cgccacgcct agagtgccga tgagggtcgg attgaagaca tgcgtgtacg
      121 agcctccgat cgagcgcgtc cggttttcaa gatattgcgc gttcaatggc acagacgagg
      181 gcgatacgga ttggccgttg aatacgttag ctcgtacgaa tagagagttc tccctgccca
      241 cccgctgatc gaagcgaatt cctccctggt ctgaattaat ttcccgcgat tgaacggata
      301 cgaaattgtt tgggaatccc ggctggttcg gagtgggata aaagtatttc aagtaatcgg
      361 tcgccacgct ggaaatacgg ctggacggaa ctatgttgcc gggatagggc tgacgcaagt
      421 aagtcccggg gttgagcggg tcgggcgttg tggagaacgg gtcatagatc tgccctgccc
      481 gcacgtttcc ggtttgcgag cccagaaaac gggagagatc gcccgagatc atctctgctg
      541 tcggaaccgt ggcaaagaac gtctgtccgc gcctggaacg aaaaccctcc caatagccgg
      601 aaatccagga gttcgtcttt cggccgtcga ttatcttggg aagaaagagc gggccgcccg
      661 cataaacccc gtactggttt tgccggtaag gcggcttcct ggtatcgaag gtgtttcttg
      721 cgtcgagttt gtcgttccgg agaaactccc aaactgagcc gtggaattca tttgtgccgg
      781 ctcgtgtcgc gatattgacg ttagctccag cccgctggcc gctttccact ttgaactctt
      841 gaatggcatc aggaggcggg ctgattgccc aaatgttagc gaatcgggag ttgttatcca
      901 gtccgtcgag ggtgaaattg ttctggttcc ccgattgccc gttaatcgcc gggctgatcc
      961 ctccaccgcc aagttgcact gtgaagccgg cctgttgtcc accgccgcga ggtgccgcgc
     1021 cgggcgagag aagaatcagt tggctgaatt ggcggccgtt tagtggtagt tctaccgttt
     1081 gctggccgtt tatgacctgg ccgacaatgg cgtcgttggt ctgaagaacc ggtgcttccg
     1141 cactcaccga tatgtgggtg gaaatatcgc cgacggcaag ctctatatcg agcgtagcgt
     1201 tttcctggat ctgcagggcg atactacggc ggatcgttgc gaaaccggac tgctcgatct
     1261 caatcgtgta ctcgccgcga gggagcgcct gaaacgtgta atagccgttc gcgttggtgc
     1321 taaccgtacg ctcgatgccg acgggttgct gtgccagttt gactgtcgct ccgggaacgg
     1381 cagcggcgga ctgatcgaat acggttccgc tcaacgaccc gcctgcggtc tggccggaaa
     1441 cgacgatggc ggctaagagg acaatggcgc acaaacgcgc cggttgcggc aagttactgc
     1501 accgaagaat tgttgactgg aaagcatccg aactgaacat ggctttctca actcctttcc
     1561 tggagatact ccgcagcatt ccaaccggct gtaacgccgg atgtgttaac agttgtccga
     1621 ggcaaccgtt gccccatccc ggcacccgcc ggcattcccg ttcccaggtt cgacactgat
     1681 gacgcgaacg tccacgtcgc gatcgcgtag cagggctaat acgcttcccg aggcgtcaac
     1741 aaccagactc gcgcccccga aagtccatcc cttccagggg ccatgcatca tgacgccgac
     1801 acaatcggcc gcagcgacgg gacacttcca ccccatcgcc ctgcggcgta cgagttgctc
     1861 tagttcttgg gcatgccctg gccacttgtc cgttctcgcg gcccatccat acggaaccag
     1921 tacgaggtcg ggtcttgccc ggctcatcac ttccgcgtac tcgtcgcgga aggtatccgc
     1981 gcagacaagc atgccgatcc ggccaaactc cgtatcgaca gcgccgatgc cgtccatcga
     2041 acccttcgag tagggaggct ccatcaattc cggcaggacg ttcagtttac ggtgtttcca
     2101 gaggagcctg ccttttcgac tgacaagaat ggctgaatca taaagacacc cgttgtcctt
     2161 ctcgtccagg ccgattccga tcatcacatc gtatttctcg gccagatcag aaatccgttc
     2221 gctgtctata ccaggtaatg gagttgcgag ccggtgcgcg tccggattct cccagcccaa
     2281 aatggaggat tccgggaaca caacaatgtt cgcgccctcg gtacacgcag acttcagtgc
     2341 atattcgatc cgccggaagt ttccctcgcg atcgccatcg ataacgagta tctggcagac
     2401 gccgacgcgg acggtccgag tcctcgataa ggtcctgcga gaaacaggaa gagtatccat
     2461 tgccgcaact atctgctctc tctggcctgc gggtcgctac agcgtgtaac ccgctttatc
     2521 cctcagaaga gtatggaatc accctgctgg ttccatcaag cgtaaccgtt tacaaggatt
     2581 tgtaaaatat ttacaaaaac tcgcggacag cgattttaaa atgaggtgcg attgcgatgt
     2641 ggcatagata ccggcacgta gctttggtgt acgatgcgag gctcgcttac gacgtaaagg
     2701 tgatgatcgg catcggcgcc tggatccacg aacacggcaa ttggagtgtg tacatcaagg
     2761 aagatgcgtt gaaagatcag catttgcccg acctcgcctc gtgggacggc gatggcatca
     2821 ttgcaaactt cgacgaccgg gcgatttttt ctgccgttac caactcgcgg ttgccgtcgg
     2881 tcgccttcgg agggggaggt tcgtgcgatc aaagccgctg caaagttccg tatttttttg
     2941 gagacgatga agcgattgca aggcttgcgg cggatcattt gttgagccgt ggattgcgat
     3001 ctttcgcata ctgcgggtat cctcgcgatg cgatcaatgg atggtcggag aggcgcgcac
     3061 atgctttttg cgcacgcgtc gcacaacggg ggttcaaatg cgaggtatat cgcagctcgc
     3121 aaagaacgga acaggagtgg gttgcgctgc ggcgttccat ctcttcctgg cttaccaccc
     3181 tgccaagacc gctgggattg atggctgcga acgatcgtcg tgggcgtcag gttctggagg
     3241 cgtgccgcgc tctcggtctt cgcgtgcccg acgaggtggc cgttatcggt gttgataacg
     3301 acgaactgtt ttgccaaatg agtagtccac ccttgagcag cgtcgaacac ggcgcgaaag
     3361 aactggggta cagggcggcc gcgttactcg aagagatgat gaccggcaga aaggccgagc
     3421 gaaggcgtta tgtgattcct ccggtcggag tggtcactcg tcaatcgacg aacgtactgg
     3481 cggttcagga gccgaaggta cgggaggcga tgagtttcat tgcggatacg gcttgtcatg
     3541 gcataaaagt gcgcaacgtg gccgagatcg tgggactatc gagatcggca ctggaaaagc
     3601 gctttaaggc gaccctggga tacaccgttc cccaggcaat tcgccgagta caactcgagc
     3661 gggcaaggtt attgatcgtg gagacaaaac ttcccctcaa gcaggttgca gacaatacag
     3721 gtttccgatc ggttcagcat cttacaaccg tgtttggcaa taccttcggc cagcctcccg
     3781 cccggtatcg caaatcgggt cacgtttgac ccggctggag tttgtctgcg ccaatccata
     3841 cccagatcgt caggggcgac attcgattgt agtttgagaa ttttctggcg cgcccggagg
     3901 gactcgaacc cccggcctac tggttcgaag ccagtcgctc tatccggctg agctacgggc
     3961 gcgcagttgt gaacctgtcg ggttgctcct cgaacccggg agcacaccgt ccattatgcg
     4021 ttgtccggct gctttcgaac aagccacccc tcgtctgccg ctcccctggt actagcgata
     4081 tccctgccag aatactccgc tttcccctca ttttgtctgt tgtacccctt caggatttcg
     4141 gagtagctta aggatgaaat tcgatacgaa ggtatagatt ctatgacgca atcatttcgt
     4201 ttgtatgcca gcgctttgca tccccgccaa tggatcgcgt ggtcggacgc gaccggatgg
     4261 gtgcagtttc cgaacgagga caatggttgg gagctcagga agtccgcccg cggtctcgac
     4321 cctgttcact tgcgcgcgac gccgctgagg gaagctgcga ataccggtat ccccaccggg
     4381 ccggtttctt tcggttcaca gcccaggaga gcggcgtgaa acccaggcac gcacctctcc
     4441 ccaattccgg aaaagttgtc aggaaaagaa agctcgtttg cctcacctgc cgcaacgtaa
     4501 agtgcatctc gcgatgcaaa ttcgtccccg cggccaaacc atagactggt tgcgactaga
     4561 cgtcgcacag ctccacggct tcggcaagcg attcgagcgg gtttcgcttg gggatgaact
     4621 cgtgcgccat atacccgctg aagcccattt ccactacagc gttcgcaatc gcccggtaat
     4681 tcagttcctg tgtgccgtcg atttcgttgc ggcccggatt gccgccagtg tgcaagtgtc
     4741 caatatattc ggcattctct cgcagcgtcc ggatgacatc gccttccatg atctgcatat
     4801 ggtagatgtc atacagcagc ttgaaataag gggacgcgac gcgtttcgcc agcgccacac
     4861 cccacttcgt gtgatcgcac atatagtcgg catgatccac cttgctgttg agcaactcca
     4921 tgcaaacaag aatgcctttg tcttccgcta taggcatgat tttcttgagg ccggtaacgc
     4981 agttctccag cccctcctcg tcgctcatgc cgcggcgatt ccctgaaaat acgataatgc
     5041 tcggcgcgcc aacggttacg gcgttccgca cgctctcccg gaaccgctcc tcaattccgg
     5101 catgattctc cttccggttg aacccgtcgg ggatggtcgt gccccctcca atcatcgtcg
     5161 gcggcaggcc gtatttcttc agcaccggcc actcggccgc cggcgccagg tcgatcgatt
     5221 tcagccctag ccgtgcacct tcccgcacaa aatcctcggc gggaatcttg ctgtaacacc
     5281 accggcaagc ggactgtttg atccgcccct tgcgctccac cggcttcatc tgtgccgcct
     5341 ttccggcgag tgcggctccg gagccggctc ccaccgtttg gaggaatacc cttcgatcca
     5401 tgccgttatc ttatgacagc tcggggttgg gtgggccttt ttccaggtcc gcgcatggta
     5461 gaatcaccct tttgcgacaa gtcatccccg catttgaggt tatctcgcat gaaatcgaag
     5521 ttctattcgt ctctttttct gcttacagga ctctctctaa cgccgccatt aggggcacaa
     5581 tcggccgatt taatcctgta caacgggaaa attttcactt ccgacccgtc acgtccattc
     5641 acccagtctg ttgctgtccg ccagaatcgc tttctcgcgg ttggtaccga tcaggacgtc
     5701 gaaaggtacg caaacgcaaa tacacggcga atcgatctta aacaaaaact ggtgattccc
     5761 ggaatcaatg attcacacac ccacttcgcc ccgatgccgc ctctcgtgaa cctggggctg
     5821 tggcccgacg gggcacctcc gccggatcct acgtttgatc aggtaaaagc tgccatcgtc
     5881 gcagccctga gcgcgcagaa ccccccggcg ggaacctggc tgtatgctga cgttggcggc
     5941 agggtctttg ccgacccgaa cgctaccagg ctcgcgctgg atcaggttgc gccgaatcat
     6001 cccatcgagt tgcgcggatg ggccggccat ggctcgatca ttaattctgc cgctatcagg
     6061 gcgctccggc tctccgaacg cgaatccgat ccggcaggcg gatggtatga gcgggacgcc
     6121 ggcggcaacc ttaccggagt gctccacggg atggccagct tcaacctacg gtcctgtctg
     6181 gcggccgccg tcgatgagaa cgtgggtgcc gcgcaaattc gtgcgatggc gcagcaggcc
     6241 gccgccttgg gcatcacttc catccagaac atgtccggcc tgccgccgaa caaaatggta
     6301 cgcatcctga accgcgccgg gcttcccatc cggtggcgcg aaatcccggt acccggcacc
     6361 atgcagaaat gccccgcgct ggatgtgccg ccccttccca ttgtgattcc ccagggaaac
     6421 gtgacgcttt ccggagtgaa gtacatcctg gacggtaccc cggtggaacg catggccttc
     6481 atgtatgaga gataccttga cgttcccggg agttttttcg gaaagctcta tttcacgccg
     6541 ggagatatcg accggcgcct ggctctgaac gcgctttctt ttccccagcc gctatttcac
     6601 gtcgtcggag aggcaacggc cgatctgctt ctggatctcg cggaacgcaa tccgttgagc
     6661 gtattgctga ggttagtgcg gccgcgcatc gaacacgggg attttctggg aaccgacgac
     6721 tatcaggcct tgcggcgtct gggcgccatc gtcgtgcaga accccacgca cttcaacatc
     6781 gtggatgtat ttatgcagcg ctacggcccg gtcaaaaccg cccacgcaca gccgttgaaa
     6841 tctcttctgc gggagcgaat tccgatcgcg ctgggttcgg acggattcct ccacccttat
     6901 atcaacctgc tgctcgcaac cgtgcatccc atcaatccgg ccgaagcaat cacccgcgag
     6961 gaggccttgc gggcatacac ctcgggctcc gcttacgcgg agtttgcgga gcgcgagaaa
     7021 ggcacgatcg ccgccggtaa actggccgac ctggccgtat tatccgacga tgttctcact
     7081 ttgccgctgc cccctccgcc tcccgctccg cccctccagt ggtttggagt tcacagcgtg
     7141 ctgaccatgg taggcggtac gatcgttcac cagacgcccg ggttgaactg agcggtttcg
     7201 caatcggagt tgctgggata tcgtagaaga cggtctgctg acttcggcaa tgatcaagag
     7261 aagagatttc ctttatctgg cgcccgcgct atgtgggatg caggccggga ccgatgccgc
     7321 gcagatggaa gccggcgccg aaccgatatc cgaaccccac ttccccagcc gtctccacca
     7381 attcgtctgg cggaattggg aacttgccaa catcgagcgc atggcgagcg tagtgcgcgg
     7441 aacgccggcc ggtcttctgc agatcggggc ttccatggga ttgccggaaa agcggaagct
     7501 caccgaagat cagcttgccc gcatctacat caccgtgatc cggcagaact ggcacctgct
     7561 gccggaggac cagatcgtcg gcctgctggg atgggatcgc cagaagtatg agtttacgct
     7621 gaaggaagac gatttcctgg acgtcaaact cggccggaaa aaaccaaagt gcgaaccact
     7681 ggtgtacgcc gagccatcgg ctcaggaaaa agcccgcgcc gcggaaattc gaaacaccgt
     7741 gcgccggact tttggaaagg ccctcgacga acccggtgaa gatctgttcg ctttcatcgc
     7801 ggacctcagc gatacgggtt ttcgaaccct gcgcgctccg ggaacgaaac ccgccgcgga
     7861 cgagatcgat ctgacgaacg gatggagcat cgcggcgggc ggcgcgttct cgcgccaggc
     7921 cgcgcgcttc cgggagtacc tgcgttcctc catgaatgcc gttgtcgcgg aaaacgccca
     7981 aaagaaaatc gtgctggaag tccgcccgcc ggaagcggcc ggccgcgaaa gcttccagat
     8041 tgaagttggc gacaaccaac tggtgctcac cggcaccagc cacgcggccg tggtcgaagg
     8101 tctgtacctg ttgcaggatc ggatggaatc gcgcgaaggg ccgttcctga agaagggctc
     8161 cattcatcgc aatgttgaat ggaatccacg ctatctctat tcgtactttg ctctgtacgg
     8221 cgatccgttg atggagccgg agcgcgatcc tttccccgac ggctatctcg aaaaactcgc
     8281 gcgcgccggc atcaacggtg tgtggatgca ggccgttctc aacacgctcg ctccctcaaa
     8341 gcggtttccc gagttcggcg aaggatggca gacacgcctg aagaacctga acgccctggt
     8401 gcaacgcgcg cgccagttcg gcgtcggcat ctttctgtac ctgaacgaac cccgcgcgat
     8461 gccgccggct tttttcgaga agtacccgga cgttcgcggc tcctcgcacc agggattgaa
     8521 cgcgatgtgt accagcgtgc cggcggtgcg cgactggctc cgggaaagcc tggctcatgt
     8581 cttcgccaac gtgccggacc tgggtgggat ctttaccatc acgatgtcgg agaaccacac
     8641 caattgtttt tcgcacggcg gtgcatgggg tacggggaca cccaatgccg gcgattgccc
     8701 gcgatgctca aaacgcaccg gccatgacac catcgccgag ctgctcgaaa catttcgcga
     8761 gggagtgcgc agccacaatg cccgcgcgga cgtgatcgcc tacgattggg gctggggata
     8821 cgcgctggcg gagaaactga tcccgctgct gtcgaaagaa gtaaaggtgc tgagcatcag
     8881 cgaatgggaa gcgccggtag atcgcggcgg tgtgcaaaca agagtcggcg agtattcgat
     8941 ttccgttgcc ggcccgggcc cccgcgccac ccgttactgg aacattgcga agcagaacgg
     9001 gaacccaacg atggcgaagt tgcagttcaa caatacctgg gagatctccg ccgttcctta
     9061 tattcccgtt acgcatctga tcctggaaca ttgcgagaac ctgacgcgtg ccggtatcaa
     9121 cggcatcatg ccgtcctgga catgcggcgg ctacgcctct ccgaatctcg ccgcggccaa
     9181 gtcgtactac tattcgcccc ggccggaaca accggagatt ctgcgggcgg tagccgtgca
     9241 gagattcgga agcagcgcgg cggatgcaat cgtcaaagcg tggcatcagt tcagtgaagc
     9301 gttccgggag ttcccgtacg gtgttcacgt ctacatcatt cccacacagc atggacccgc
     9361 taatccttta cgtctgacac ccaccggata ccggcccggg atgatcctgt ttccgtacga
     9421 cgattacaaa gcctggtcgg cgaagtaccc tccggaggtg gtgcagcggc aatttgccaa
     9481 aatggcttcc ctgtggaacg cggggctccg gctttttgaa aaagcgatac cggaagtctc
     9541 gccggccaag aaaaggaatg ccgaactcga tctcgccatc gcgcggacct gcgcccatca
     9601 cttcgagagc acggcgaacc aggtggagtt ttatatgctg cgtgacaagt ccgatcccgc
     9661 ggcgctgcgg cgcatgcggc agctcgccga acaggaaatc gatcttgcgc ggcggcagta
     9721 tcccatcgcg cgcaagcact ccgtgattgc atacgaagcg tcgaaccact actattacac
     9781 tccgttggac cttgtagaga aagtgctcaa ctgccggcac gtgatttcgg aaatcgaccg
     9841 gcggaacagc gcctgaggag gcaaattgaa tcaagtgcga attggactgg tgggctgcgg
     9901 actgttcggc gagagtcatc tgatgggctt ccgcgctgtg cccggtgcgg aggtcgcggc
     9961 ggtttacgat gtggattccg cgagagcgcg ggaaatggcg cgtgtttttt ccatcccccg
    10021 cgtgtgcgat tctctcgacg agttgtgttc gctcccggaa ctggaggcgg tcgatgtcgt
    10081 cactccggaa gaactgcacc tggaacccgt cctgaaggcc atcgcgcgcg gcaagcacgt
    10141 gtttgtcgaa aagccgctgg cgacggatct ggaacattgc agccagatga tccgcgcggc
    10201 aaacgacgcg ggcagaatcc tgatggtggg acagcttgtc cgcttcgaga cgaaggcggc
    10261 catgttgaag gatgaagttg catcgggcaa gctgggcaac gtgatctcca tgcatgcgcg
    10321 gcgcaatcgc ccgaaagcgc tgctggagcg gtacggccgc acgcacccgg tcgtcgaaaa
    10381 ctcgattcac gatatcgacc tgatgctctg gtacacgggc cggcctgtgc ggcgcgtgcg
    10441 cggctatgac cgtaaagcta ttgccacccg gcatgccgat accttttggg gaatcatcga
    10501 attcgaaggc ggccccatcg gcgttgtcga aacaatatgg ctgcttccga agcccgccgg
    10561 tgttctgctc gacgacgcat tccagttgat tggagaccag ggcattgcga acatggccct
    10621 gtttcccggc agcctgtctt tctggcggga caccggtttc gaaattccgg atatcggata
    10681 cgacccgcgc gtgcacggcg ccgcgctcgg agcgctccgc gacgagctgg cctacttctg
    10741 cgattgtgtt cgcggcgggc gcatgccgga aatcattacg gcggtcgaag ccaagaacgc
    10801 cgtgcgcgtg gccatggcct tgatggagtc cgcgcacctg gatcaggatg tcgaaatcaa
    10861 gagctggaat taacccatgc tcacgagccg tttcgaatga aatcacagtt caaagcactc
    10921 tttccgagag cggtcgaaac cgtttatctc gacaccgccg ccgagggcct gcccgccccc
    10981 ggcctggcgg aaagcttcgc ggaatactgt cgggataagt cgctcggcac tcccggccgc
    11041 aggcggtttc atcgcgtgga ggcggaaacc acctcgctct gcgcgcggct gctcgatacc
    11101 tcccccgaaa atgtcacgct gttgtcgaat gcgtcggaag cgttcaacgt cctggcgggt
    11161 tcgctcgaat ggaaggcggg cgaccgcgtc atcatcagcg acctggagtt tccctcgaat
    11221 gtctatccgt ggctgcggct cgaacaggtg ggtgtagagg tcgtggtggt ctcgggcgat
    11281 ggaggcgcgc tgcggttcga ggatatcgag cggcatataa acggctccac gcggctcatc
    11341 tcgctcagcc tggtcagcta ccgcacaggc gcgtttctcg attgcgtgcc gcgcctgtcg
    11401 gcggaggcac accgggtagg tgcgtttctc tccatcgacg ctacgcaggc gctcggccgc
    11461 ttgccgctca acgtggaggg catcgattac ctgatgtcga gcagtttcaa gtggctgctt
    11521 ggtccgcacg gattgggcgt ggtgtacgta tcgccggaat tgcgggagcg gctgaatccg
    11581 cgggccgtgg gctggtattc cgttcgcaat tgctttacgc ccgaccgttt cgaacgctat
    11641 gagctcaaga gcggcgcggc gtgcctgtcg agcggaatgc ccaactttcc ttccctctac
    11701 gcgctccgca acagccttca attcgtgttg cgcaccggca tcgattctat ccgcgatcgt
    11761 ttgctcgaac ccgtcgcgct cctgcgccgg cgccttgccg ccgcgggact ctcactgctc
    11821 acgccggaag atccccgcct ggcctccggc attgtcgctt tcgaacatcc gcatgcggcg
    11881 cagatcggca gcgcgctcga agaagaaggc gtcattgtct gggcgggcga tgggcgtgtc
    11941 cgggcttccc tgcatctgta caacgacgag gaggacgtgg atcgctacgt ctccattctc
    12001 gacggcgttc tcaaaaagga aaaggtcgcg catgggtgaa atggcgtggg gcggactggc
    12061 ggcggtggtt tttggaggcg tgctgcaagg cgccttcatg ctcccaatga aatggacccg
    12121 gcgctgggcg tgggagaaca cctggctgat cttcgcctcc accgcctacc tgctctgccc
    12181 gtggctgctg gtgctgacga ccattcccca ggcgttcgat atctatcggg aaagcagcgc
    12241 cgccgccctc gcgatcgtcc tgcttttcgg ccttggctgg ggtgccggag ccgtcacgtt
    12301 cggcctgggc gtggatgcca tcgggttggc gctgggattc gccatcatcc tcggaatcgc
    12361 cgcggccgtg ggcgcccttg tgccgctgtg gatgcttccg gtttcgccgt cggccgccaa
    12421 actggccgtg acgctcgccg cccttgccat catgctggtt ggagtcgccg tctgctcgct
    12481 ggccggcaaa tggaaggaca agccggcgga agggaaccgc cggtcttcct accagaaagg
    12541 tgtgctgatc tgcctggttt ccggtttgct gtctgcctgt ggcaacctcg gattcgcctt
    12601 cgggcagccg attatcgatc gcgcacaggc ttcgggtgtt ccaccgtatc tcgcctccaa
    12661 catcgtgtgg gctttgctca ccatcgctct gtttctgtgc aatgccggat atgccctgct
    12721 gctgctccgg cgaaacggaa ccgccaagca atttcgcctg ccgggcacgg gcaagtactt
    12781 cgctttcggc tgcctgatgg gcgtcatgtg gatgggcggc tacatgtttt acggggcggg
    12841 ggcgcggcgg ctgggcgagt tgggtccgtc tctcggctgg gtcattctga tgagcggcat
    12901 ggtggtgacg gccaatgtgc tcggcgttct caccggagaa tgggcgggcg cgccgcgatg
    12961 ggccacccgc aaactggtat ggggaatcgc actgctgctt gccgccatcg tcgttttagg
    13021 ctacgcaaac tccctcgcat gacatttttc gagttcggat ggccctggcg tcgcgaggcg
    13081 ttcgggccta aaatgtagat gccacgggcg gcgtaacgga ccgtggcgga ggtggcatcc
    13141 gatggagacg cagactcttg aattcacgcc ggagcaactc gcgctgatcg tggagctgct
    13201 cgaaaacgaa cggcgcaccc tttctctcca gacgcggcat tcgttcagcc atacctaccg
    13261 cgcaacgctg caaaccaagc tgacaatggt ggatgagctg ctgaaccaga ttcgccagca
    13321 tcagccggcg tgaccctggc cggaaaaaac tgacgcgctg gtattgtgcg ccggaaatcc
    13381 ttcggagtag gatagtccga ttcccctgcg aaggaacgtg gagatgttct attccggcga
    13441 acactggaga tctgttccca atagcgaaaa gcctcgcctt ctgaaaagaa tcaccaacgg
    13501 ccaccggtat cccgcgctga aggatccttt cgtttgggtg gaaggcgagg cggcgcagcc
    13561 gggcgagctc cccttaaacc aacaactgca cgtccgcgat attctccgcc gtgagccgcc
    13621 gttgtccttc tatccgggct ggagtttgta tgccctcgat ttggatatcg ctcaggatct
    13681 tggccaacag gcgcaggaac gctacgagtg gctgcgcgtt ttctttctgc ttcgggacga
    13741 agacggtgcg ggcggcgagg catccgccgt cctgctcgac ggccgcggtc cggcgattca
    13801 cgaattcaac aagcggatcg gtttggaact gactcccgat acggctctcg agtacctcga
    13861 tttctttacg gcttccgttt atggcgatct cgggccgttt ctgatcctga gcgccgggga
    13921 ggatctgatc gatgtttccg cccggaatcc cccggcggaa gcattcctcc agcgattcgg
    13981 agctaagtcg agcgaagttt tcgcgaaggc ggagccgcag cgcggcgagg gtagcggaac
    14041 gacaattgcc gcagcgcgcg ggaccatacg cccgccgtac caggtctctt tgcagcagga
    14101 agaccaaccg gaaggagaca ggcagacctt tgctgttgcc ggcgtctcca tgcaatatgg
    14161 cggaggtctg ttcgacagcg cgctggaggt tgcccgggat cgatcctatc tcggcggaat
    14221 cgtaatgctg ggggataacc tcgtcgccaa cgacatccct atcgtgtcgt acgaggttgt
    14281 tcgccagcct ttgttgagca tcccctgcct ggcccggcgg cgccgcaatt tcgggtataa
    14341 gaccatgctg ccccaagcgt ttgcatcggc cggccgggaa gccttcgccc gttcgcacgt
    14401 ttttgttccc ggtgatctgg atcttcgcgg acgtaccttc aagaatgcgg tcaaactgga
    14461 caactggatg attgaaggtt cgctgctgtt ggaccgcgcg acgttcgaag acgatctgat
    14521 tctccagcat tgttcgatcc tcggaggctt ttccgccagg cgcatggttt gccagggccg
    14581 gttccgagtt ctctccaccg atgttctcgg gaattcttcc agaagggatg cgccgaaagg
    14641 catggttctg cgcgccgccg ttctcagccg tcctgcgagg ttgaaacgtg tccgtgttgc
    14701 cggagaactc gttctgcgtg aggccgagtg caaaagcaag ctggaaatct tcggactgac
    14761 gacgcgcccc agcacaccgt accccaacga aaagacagtt cccggcgacg ttgatgccat
    14821 tgggtgtaaa gtacagcgct cgttcctgat gaagcccgcc aagcaacaga gtctggccgg
    14881 agagacagaa gcgcctaacg acttgcaggg ccggtttaac gccgatcatt gcgaggttgg
    14941 ggatgaactg gaactcgcgg gctgcgagat cgccgggggg ctcaccttca ccgatgcgca
    15001 ggtgtcaggg caggtcactc ttgccggcgt tgtctcgaaa ggtggagagg atggaagggc
    15061 catcgatttg tcgggtgcgc ggttggcggg gggcgtatgg atcggcagtc accgggcgga
    15121 gggtaaaaat accagaacca taagcgggac ggttacgctg cgcggttctc aaatcggctg
    15181 gctggcatgc acggatgtgg aggtgcgggg ctccctggat ctcactttga tgacctgtcc
    15241 gcagccggtt gaaattgatg tctgctccat ctccgggtct ctccaactct ccggcatttc
    15301 ggcccattcc gaagtccgga ttcttggtgt gcatatcggc ggcaggatag agatcttttc
    15361 cggtgagatc ggcagtctgc aaatcaggcc cggtgtctcc tatacgatgg aggcttcccg
    15421 atggaagctg accggatttc acgatctgta cgcacaggac ctggaggtcc gcggcgactt
    15481 ctcgtgctgg ggtacagagt gtacggacga cttcgttctg gattcgtgta ccgtcagtgg
    15541 ccggtgcagc ttctggcgcg atccctggcc cagaattctg agttggaacc atttgcccgt
    15601 tgcacgatgg aaggaaccca aaccaaacgc gctgcgtgtt tgcacttcta tcgcgggcaa
    15661 gctggatttg caaaccttgt ccgcaacttc catcgatc
//
LOCUS       DASTMK010000595         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_189921, whole genome shotgun sequence.
ACCESSION   DASTMK010000595 DASTMK010000000
VERSION     DASTMK010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189921"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFQ79_24485"
     CDS             <1..274
                     /locus_tag="VFQ79_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142901.1"
                     /translation="TDYTELLASAGKHSPDILITCINGGFNNLSHFEAAQLASFIKPQ
                     VAIPCHYDMFPDNAVAPGQFRASLALKAPEVRYKQLEHGVPFIYSR"
     gene            284..1333
                     /locus_tag="VFQ79_24490"
     CDS             284..1333
                     /locus_tag="VFQ79_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142902.1"
                     /translation="MNRRTALSMILPAWVAAQPPRILPVRGMYSSPKAFWDKGARLDQ
                     YGVNALFVHGGSITPALMERAAAEGARVYAEFPTLNGKGYVEKHPEAWPVDERGEKSP
                     PATWFLGACPTEPAFRAWRMQQLNDLLDRFELAGVWMDYFHWHAQFEDPKPVLPETCF
                     SPTCLEGFEKAAGIRLPSGSTAARARWILDRHEDRWREWRVQQLMDWAREVKDILRRR
                     RPDALLGVYHCPWTDEEFEGARRRVLGLDLDALSKVVDVFSPMVYHGRMHRRPEWVGE
                     YVEWLSGKLPRSAKIWPIVQAHNDPQVITAGEFEKVLRLAAGGTSSGVMMFTAHSVAS
                     DEGKMEAMRRFYTGQ"
     gene            complement(1340..>2462)
                     /locus_tag="VFQ79_24495"
     CDS             complement(1340..>2462)
                     /locus_tag="VFQ79_24495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0142903.1"
                     /translation="GYDFDTWREGTGEKMDASRFPERAPEDFAPASRAEWESQKQKIR
                     EAVKAVLGPEAPHVPFEAKKCLGGMIWAGEGWLSTLFGRPTDERHWQQRLAASGMGVA
                     TVPFGDGLAGRLFYPADADGKPKAGPWPVAVWLHPYAYSTGWSARSPWNPNATDYLAD
                     QRPSFASLVRRGVAVFAFDQIGFGSRLHQAQKFYERYPQWSLMGKMVKDTRDAIEALA
                     AMEEIDASRISLFGYSLGAKVGLLTATFDERVRAVAAVCGVEALRLNSPGTEGLRHYS
                     HIHGLLPRLGFFIAREARAPFDYDEIIALAAPRPVLVIAPTRDRYAPVEDVRREVEAS
                     RRVYRLMGRDEALEFETPTDINRFSRATQERVFDWLAAR"
BASE COUNT          452 a          814 c          738 g          458 t
ORIGIN      
        1 taccgactac accgaactgc tcgcttccgc cggcaagcat agcccggaca tcctgatcac
       61 ctgcatcaac ggggggttca acaacctttc ccacttcgag gcggcgcaac tggcatcctt
      121 catcaagccg caggtcgcta taccctgcca ttacgacatg tttccggata acgccgtggc
      181 tccgggacaa ttccgcgcgt cgctggctct taaggcgcca gaggtacgat acaagcagct
      241 cgagcacggc gtgcccttca tctattcgcg gtaatctaca ctcatgaacc gccggacagc
      301 gctttccatg attctgcccg cgtgggtggc cgctcaaccg ccgcgtattc ttccggttcg
      361 cggcatgtac tcgagtccga aagcgttctg ggataaaggc gcccggctcg atcaatacgg
      421 cgtaaatgcg ctgttcgtac atggcggctc gatcacgccc gccctgatgg agcgggccgc
      481 cgcggaaggc gcccgcgtgt acgccgaatt cccgacgctg aacggcaagg gatacgtgga
      541 aaagcatccc gaagcatggc cagtcgacga gcgtggggag aagtcccctc ccgccacgtg
      601 gtttctggga gcttgcccga ctgaacctgc gttccgcgcc tggcgcatgc agcagctcaa
      661 tgacctgctc gaccgatttg aactcgccgg cgtgtggatg gactatttcc actggcacgc
      721 acagttcgaa gatccgaagc cggtgctccc ggagacctgt ttctcgccga cctgcctgga
      781 gggtttcgaa aaggcggcgg gaattcgcct gccgtctggc agcacagccg ctcgcgcccg
      841 ttggatcctc gaccggcacg aagatcgctg gcgcgagtgg cgcgttcaac aattgatgga
      901 ttgggcgcgc gaggtgaaag acatcctgcg gcgcaggcgt ccggacgcgt tgctgggcgt
      961 ctaccattgc ccgtggacgg acgaggaatt cgaaggtgcg cgccggcgcg tcctcgggct
     1021 cgatctggat gcgctctcca aggtggtgga tgttttttcg cccatggttt accacggccg
     1081 catgcaccgg cgcccggagt gggtcggcga atacgtcgaa tggctttccg gcaagcttcc
     1141 gcgcagcgcg aagatctggc ccattgtgca ggcccacaac gacccacaag tcatcacggc
     1201 gggcgaattc gaaaaggtgc tgcggttggc ggcaggcggg acatcgtcgg gcgtcatgat
     1261 gttcactgcc cattccgttg cttccgatga aggcaagatg gaagccatgc ggcgcttcta
     1321 taccggacag tgattccggc taccgcgccg ccagccagtc gaatacgcgc tcctgcgtag
     1381 cgcgcgagaa acgattgatg tcggttggcg tctcgaattc caaagcctcg tcccgcccca
     1441 tcaaccggta gacgcgccgc gatgcctcta cttcgcgtct tacgtcttcg acaggcgcgt
     1501 agcggtcgcg ggtcggggcg ataaccagca ccgggcgcgg cgccgccagc gcaatgatct
     1561 catcgtaatc gaacggcgcg cgcgcctcgc gcgcaatgaa gaagcccagc cgcggcaata
     1621 acccgtggat atgcgagtag tggcgaagcc cctcggtccc ggggctattc aggcgcagcg
     1681 cttccactcc gcacaccgcc gccaccgccc gcaccctttc gtcgaaggtg gccgtaagca
     1741 gtccgacctt tgcgcccagc gaatagccga acaggctgat gcgagatgca tcgatctctt
     1801 ccatggccgc gagtgcctcg atggcatcgc gcgtgtcttt caccatcttg cccatcaacg
     1861 accattgtgg ataccgctcg tagaacttct gcgcctggtg cagcctcgat ccgaagccga
     1921 tctgatcgaa tgcgaagacg gcgacaccgc gccgcaccag agaggcgaac gacggacgct
     1981 ggtccgccag gtagtccgtt gcattcgggt tccacggcga gcgtgcggac cagcctgtcg
     2041 agtaagcata cgggtgcagc cacaccgcta ccggccacgg accggctttg ggtttcccgt
     2101 ccgcgtcagc gggatagaac aatcttcctg ccagcccatc gccgaatggg actgtagcga
     2161 cgcccatacc cgaagccgct agccgctgct gccagtgccg ctcatccgtt ggccgcccga
     2221 acagcgtaga cagccatcct tccccggccc agatcatgcc gcccaggcac ttcttcgcct
     2281 caaagggcac gtgcggcgcc tccggcccca gcactgcttt aacagcctca cggatcttct
     2341 gcttttgcga ttcccattcg gcgcgtgagg ctggcgcgaa atcttccgga gcccgctccg
     2401 ggaatcggga agcatccatc ttctctccgg tgccctcgcg ccaggtgtcg aaatcgtagc
     2461 cg
//
LOCUS       DASTMK010000596        16086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_446217, whole genome shotgun sequence.
ACCESSION   DASTMK010000596 DASTMK010000000
VERSION     DASTMK010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16086
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446217"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFQ79_24500"
     CDS             <1..466
                     /locus_tag="VFQ79_24500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142904.1"
                     /translation="PAGAGNVYAEPAAVNFQSNDRYPQMVFAFLDTSTRDGLGGRFAV
                     PKNYAGSPKIVVDWSTTATAGNAVWDFDYTAVGGDAAESYDPSADQQSVTVTDAASAT
                     ARRRQSCEMSLTASNLAPDDTVQFTLFRDGAGGDTIAATLYLFGLYFEFSDV"
     gene            466..2031
                     /locus_tag="VFQ79_24505"
     CDS             466..2031
                     /locus_tag="VFQ79_24505"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEU0142905.1"
                     /translation="MALQFQSTAAKVNHGSGASIDGATAWTVCMWVRPAAVSGNGQFM
                     HKQQDGLGHSLYRDGAQIGGYTSCAGYSYDERISEDVLTAGQWSFVAWTYSFADKHRI
                     YHGTLGSAPAETPYSITDNSGSGAPADDSAYPLEVGNYQDSYPFGGSIAGFQYFRRVL
                     TPAEIRLVWMRPANAMLFQPALWSEYHGTGAQADWSGNGNAGTVSGGATVANHVPLGP
                     PFGWDDATTWAVVSTGTTHALAGYVAGWSSSTATLKARLHLAGRAAGQSSQVGVFRAR
                     KVARATAAGNSSSSAVIRAIRGARATAAGASVGRGLVRLVRAARGVASGASSAAAVWR
                     IRSALRGAASGVSWAVAIVHGAAGAIISLFGRTQGASSSVGTLRVARRTRATAAGAGS
                     ADAMLRIARKLAGKTVGSAFGQGVLRRMRAVGGIAQGSSTAHGKLALIRRLIGAAAGS
                     GETAGRMAGRRVLRAVAAGKAGLRGVLTLIKANFRVSAERTIVVPYEDRIIACEAEQR
                     LMTPPAENRIIQV"
     gene            2036..2308
                     /locus_tag="VFQ79_24510"
     CDS             2036..2308
                     /locus_tag="VFQ79_24510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142906.1"
                     /translation="MKTFIKHANAVKDYGFDVTKWLAGDTIESRTWSVPAGLTKGQEI
                     ADGPVSVVFLGGGEVDKTDLSTYLVSLTITTAGGRTEVFPFRLRII"
     gene            2305..2526
                     /locus_tag="VFQ79_24515"
     CDS             2305..2526
                     /locus_tag="VFQ79_24515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142907.1"
                     /translation="MTDNLRTIDRLLYWLGVYVVMLFVLAWAAGKPGEAIPVLTNLIS
                     GFAGAAFAIMRLGKEQRFERFSKVDKDQK"
     gene            2711..3376
                     /locus_tag="VFQ79_24520"
     CDS             2711..3376
                     /locus_tag="VFQ79_24520"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0142908.1"
                     /translation="MSIDLSSLGPADLEALHGVLAKLKGGADNSDVKKRRKRPKKQAI
                     QYLEEDEIERLFRAVKDPRDKAILRVAYHRGLRASEIGRLDLSDYRVKDDRLQVNRLK
                     GSASGEYHLCANEVRALKAWLKIRGPAPGPLFPARTGKGISRQMLDVLIRRYGAAAGL
                     PCEKCHMHALKHSCGTHLLNRGESIEDVQDHLGHRNIQNTLVYAKLTNRRREQRDKRL
                     RDW"
     gene            complement(3417..3557)
                     /locus_tag="VFQ79_24525"
                     /pseudo
     CDS             complement(3417..3557)
                     /locus_tag="VFQ79_24525"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3587..3928)
                     /locus_tag="VFQ79_24530"
     CDS             complement(3587..3928)
                     /locus_tag="VFQ79_24530"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU0142909.1"
                     /translation="MSGLESRIDEYPFIVRPMTADEGSGYLIEYPDIPGCISDGASPE
                     EAIRNGKDALRSVLLTKQEFGDPIPLPGERSVMPVPAKLRTRLDERAREEHTDSSALA
                     AKLIAEGLDAA"
     gene            complement(3925..4164)
                     /locus_tag="VFQ79_24535"
     CDS             complement(3925..4164)
                     /locus_tag="VFQ79_24535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142910.1"
                     /translation="MPSAMKTLQKMRNNPRDWRIEDLKAIADHLGIAYRQKGTSHVTF
                     RAPNGEVLPVPAARPIQPVYVRLFLKLVDSMEKAE"
     gene            4292..5941
                     /locus_tag="VFQ79_24540"
     CDS             4292..5941
                     /locus_tag="VFQ79_24540"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEU0142911.1"
                     /translation="MSREPLAATWKNKLFFGDNLVILHEQIPDESVDLVYLDPPFNSN
                     ATYNVLFAERSGAKSSAQIRAFDDTWQWSMESELAYHETVTRGGKLADLLQALRAFLG
                     QSDMMAYLVMMAPRLAELHRVLKRTGSLYLHCDPTASHYLKLLMDAVFGPENFRNEIV
                     WKRTSSHGNVSANYGDVIDTLLYYSREPKPIWNQLYVPYSEDHIRNKFTYRDEHGRAF
                     TTSDLRNPSLRPNLIYEYKGYKPHPNGWSISRQKMEQYDREGRLWFPSDPDGRIRLKR
                     YLDEQPGQKVQNLWDDVKPINSQAQERLGYPTQKPEALLERILRASSREGDVFLDPFC
                     GCGTAVHVAEKLRRRWIGIDITHLAITLIRHRLHNAFGRDLEPYEVIGDPKDVESART
                     LAVLDRYQFEWWALGLVDGRPAHDKKKGADQGIDGLIYFFDDGTNVAKKAVVQVKSGH
                     VTRNQIGDLNHARQREKAEVGVFITLEDATEPMRREACSAGFYQPDYFPGLKVPRIQI
                     LTIAELLAGKKVELPMAAVPATFRKAARAHKNAEPQQTSIF"
     gene            complement(5975..6145)
                     /locus_tag="VFQ79_24545"
     CDS             complement(5975..6145)
                     /locus_tag="VFQ79_24545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142912.1"
                     /translation="MREKGINNSELARRLGVRETVVRRMLDPEHETKTEKLQAALEVL
                     GKRIVVGVEDAA"
     gene            6198..6605
                     /locus_tag="VFQ79_24550"
     CDS             6198..6605
                     /locus_tag="VFQ79_24550"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0142913.1"
                     /translation="MAAVAAFDSSAYRKLLARTLPKAIKTLEENERMTAELERLDNLD
                     RPLTAEEKTFAELVTVLIQQFETSRYPLGHAEPVEALRVMMEQHNLRQRDLVPVFGSS
                     SVVSDVLNGKRGISKSHAKKLAEFFHVPADLFI"
     gene            6711..7268
                     /locus_tag="VFQ79_24555"
     CDS             6711..7268
                     /locus_tag="VFQ79_24555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0142914.1"
                     /translation="MPDSKPKSETPSEVWLPRDKALANLARSIDVLQRHVKAGHIRKR
                     GLGATAVYNAQDILAVRTGAHKMHIESGGQAAAAVPLQAQADAWSHLAGFLANLTKTV
                     AEINQQAALPAPEPAAEDVPPFGYLSVAEAARLVRMPRSWILEQIESGRLAYAVTSRG
                     GYRVRRADLEAMPAPERVRKHAAGR"
     gene            7309..7641
                     /locus_tag="VFQ79_24560"
     CDS             7309..7641
                     /locus_tag="VFQ79_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142915.1"
                     /translation="MGNQTSARKRISYANLLRQACAFRRNLASAHPAIALPAKLKSIQ
                     TQVQAYTDWLATLQTKGLFAVTPPKRPAAKPKAMAAGVGAPTAVAGSTGSRRRGAGRA
                     SKARRAQA"
     gene            complement(7700..8350)
                     /locus_tag="VFQ79_24565"
     CDS             complement(7700..8350)
                     /locus_tag="VFQ79_24565"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU0142916.1"
                     /translation="MPQVSPAVKIFISWSGEQSRTFAEELKPWLEQVLPGTDVWLSSE
                     DIDKGTIWFTEIIGELERCNCGVICITRENHLAPWIHFEAGGMVKGLGKSRVATILLD
                     IDYPELQQPLNQFNGLRINSQSAFHLVKSFNKLSDRPIKDRVLERTFEKFWPDLDNAY
                     KLLFPDSHENARNTDQPVHIVPKPTDTILAPPVRRKRRKSEKSQDSQPGLFNGMLE"
     gene            complement(8398..8871)
                     /locus_tag="VFQ79_24570"
     CDS             complement(8398..8871)
                     /locus_tag="VFQ79_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142917.1"
                     /translation="MIHYYTLIGQTPVPCDDLLEWARWFEENDRTVMRTRILGLCEVS
                     TVFLGLDHGLPFRLDGPPVLFETAVFWLGEDQWEMARCSTWSEAEEQHRRLCMEVARP
                     RRVLVFIRRTFRDAIKAAKDDWRSAWRELRGIEPSEIEVLVQRLRNQVHRYGGMW"
     gene            complement(8868..9272)
                     /locus_tag="VFQ79_24575"
     CDS             complement(8868..9272)
                     /locus_tag="VFQ79_24575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142918.1"
                     /translation="MRTITDHVVSGDQAVQLKIAVTDEPGQGGANHRYEITGMDATTN
                     PSRSQTEPDVSFCRQVVLFQNGPIKEFGVNGITQEALLAIVIDRLRSFQAGPFSCRDN
                     AIALTHCKEALMWLQRRTRERIKRGVEGTNQK"
     gene            complement(9335..9574)
                     /locus_tag="VFQ79_24580"
     CDS             complement(9335..9574)
                     /locus_tag="VFQ79_24580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142919.1"
                     /translation="MNPNKEPDNYDILWHQCGVSPRGEPFVQLLRGDQIIGQMSPEQA
                     RDFARAITEAAEAAEPPPVYGGGPPASRGRSSANG"
     gene            complement(9571..9861)
                     /locus_tag="VFQ79_24585"
     CDS             complement(9571..9861)
                     /locus_tag="VFQ79_24585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142920.1"
                     /translation="MSQTWDEHAPQHKATRRHFDELDFSLDPRTRLLAKFGEALTVIK
                     TQPEKCHGGPEYVRDLRNMLEWAIGVIECDVHNQQVDEICQALIGKRLKDLK"
     gene            complement(9858..10118)
                     /locus_tag="VFQ79_24590"
     CDS             complement(9858..10118)
                     /locus_tag="VFQ79_24590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142921.1"
                     /translation="MPDRPDLFDVAREDLKRQSGRITVALTYTEAWSVLGQLQLAARH
                     PGNTGPGARIARQFAELLQQEVSTTPMLAAIADLGWNPEHDT"
     gene            complement(10111..10335)
                     /locus_tag="VFQ79_24595"
     CDS             complement(10111..10335)
                     /locus_tag="VFQ79_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142922.1"
                     /translation="MTYTEQGDRVTLEMTRENFCQLLFLIVYATGAATREGDKTLFCH
                     WIKFTNDLNATNPRWTPIEIPEEFRTMKDA"
     gene            complement(10332..10541)
                     /locus_tag="VFQ79_24600"
     CDS             complement(10332..10541)
                     /locus_tag="VFQ79_24600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142923.1"
                     /translation="MILLRMDTWERIRADFTAAEKLELNLAVAGEAVCPSGAFIHEEL
                     LPAELREKLRRALQPQRAAVGGPIQ"
     gene            complement(10538..10738)
                     /locus_tag="VFQ79_24605"
     CDS             complement(10538..10738)
                     /locus_tag="VFQ79_24605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142924.1"
                     /translation="MMIVEDPHTHERRECLYFPKWQATKPWLCGACNRGWMYGLLDGE
                     QCPHCGARTVRDAGVHVRGGAA"
     gene            complement(10735..10881)
                     /locus_tag="VFQ79_24610"
     CDS             complement(10735..10881)
                     /locus_tag="VFQ79_24610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142925.1"
                     /translation="MKRPEIPCALCGRRFFSTLDYVRLPGGKRRCRDERLCAERQKLA
                     KKEQ"
     gene            complement(10868..11113)
                     /locus_tag="VFQ79_24615"
     CDS             complement(10868..11113)
                     /locus_tag="VFQ79_24615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142926.1"
                     /translation="MTSRWRSYAAPIIRRVLADTAGRPEAEIRAALRDAYPFGARRNH
                     PYQIWLDEIAVQTGRKRRHPPDPRQQTLYAEESDETP"
     gene            complement(11110..11421)
                     /locus_tag="VFQ79_24620"
     CDS             complement(11110..11421)
                     /locus_tag="VFQ79_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142927.1"
                     /translation="MTIAEAQQVLRPPLRFGDPEQIQAHELWVGALLALEHFLGCEEC
                     DCSPPVCPVRLATPRAVWVAAMSIVEAMDDITLIAEFRKRGYDVADEGTRTGTAGGGS
                     S"
     gene            complement(11421..11621)
                     /locus_tag="VFQ79_24625"
     CDS             complement(11421..11621)
                     /locus_tag="VFQ79_24625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142928.1"
                     /translation="MIPTPSEVLAAITAVANAAAAAFTWMQTAEGRKVVNRALEDRAA
                     WDAFWRRAGDAFIRFMKGEYFR"
     gene            complement(11618..12085)
                     /locus_tag="VFQ79_24630"
     CDS             complement(11618..12085)
                     /locus_tag="VFQ79_24630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142929.1"
                     /translation="MSVSQSADRPTDTLPPEPELEAIRQAIPEDLIEDLGEVPSRRLL
                     QRIHAKLRGAPVEWLTQRIRLRRRIITSLGVIEHFAGDIGNTYQEIQRRRQRSSAAPL
                     PPAPSSPEDIAALIAEARDILSDPQAGSGLHQWARDTLAAHANKTKTAGGGSS"
     gene            complement(12094..12738)
                     /locus_tag="VFQ79_24635"
     CDS             complement(12094..12738)
                     /locus_tag="VFQ79_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142930.1"
                     /translation="MSTGTNNKVPGWLAQLGVREGEYFVDSKPRARRAMKSQAFSPAA
                     RIHICLGLHTMAFRQELAVKVEGGKSVPLTPQDICAETGIRREHFRRHMTELEGYGLA
                     DCKGSTKGRVEIYAWAVPRNVDVKKIVTARGDNFEWCPADLRPLLKHYRIRFSEDFVI
                     ARGDILELERLARVTMEAELSLRAYAKGLRAQAPPNKDERKGTGLRSRRRRLRP"
     gene            complement(12735..13043)
                     /locus_tag="VFQ79_24640"
     CDS             complement(12735..13043)
                     /locus_tag="VFQ79_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142931.1"
                     /translation="MPSADRLLAWLQRIAPTLKEGEVRLLLYFIGESPDSRNTLMRRS
                     FEALSQHTGLSPRRLEDAVDSLTERGFIEVHAGSGQTPNGYVLIGRKVRTKSRRGGSE
                     "
     gene            13250..13399
                     /locus_tag="VFQ79_24645"
     CDS             13250..13399
                     /locus_tag="VFQ79_24645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142932.1"
                     /translation="MLANCEHSRTEVIARRDGVDYVRCLGCGQVFEADDLDPVPASEE
                     DDQAL"
     gene            13602..14693
                     /gene="yvcK"
                     /locus_tag="VFQ79_24650"
     CDS             13602..14693
                     /gene="yvcK"
                     /locus_tag="VFQ79_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEU0142933.1"
                     /translation="MTGSSVNSSVQRTRNALKIVAIGGGTGLSALLHGLKPHVFESPN
                     GSPYVRRPRLEITAIVTVTDDGGSSGRLRRQLDVLPPGDIRNCMVALSEDEALMSRLF
                     QYRFSSGHGLRGHSFGNLFLTALTHLTGDFPLAIKLSSDILASVGRIYPSTAENVVLR
                     ATLEDGSTVLGETQISRSTSRIERISLEPRRCKPLKEALRAIEEADLITLGPGSLFTS
                     VVPNLLVQGIPSAIQHSPAIKAHIANLMWQPGETMGFTVADHVDVIHRHARRKILDCV
                     VVNTHPIAEQQRRRYARARVKSVEVEPDRLNAMGVSVIGRDLLAPVSGKAAIVRHDPA
                     ATAAVAIELAAAGRRRRNRLAPQQKHLVG"
     gene            14703..>16086
                     /gene="glmU"
                     /locus_tag="VFQ79_24655"
     CDS             14703..>16086
                     /gene="glmU"
                     /locus_tag="VFQ79_24655"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU0142934.1"
                     /translation="MLTDLRVVVLAAGLGTRMKSKRAKVLHRAGGRTLIDHVVGTALE
                     LTTPEQVAVVVGHQREYVEELMAPRRVRFVHQQEQKGTGHAVMVCRDTLESHTGLLLV
                     LYGDCPLLSVETLRALIERQRQSSAAATVITTTLEDPTGYGRVLLDAGGSVQAIVEQK
                     AATPEELQVRVINSGIYCFRSDLLWESISSIQPDNPAREYYLTDIVKIFHASGHKVNA
                     MHLDDASEVLGINTRIELAEVDRIFRERKVRQLMLDGVTIEKPETVTVDLDATVGPDT
                     VIEAFARILGGTQIGEDCTVGAYSIVCDSHIADGVTIHPFSVVELSTISGGAHIGPYS
                     RLRMGSHVEAGAHIGNFVELKKTRIGPGSKANHLAYLGDSEIGPGVNIGAGTITCNYD
                     GVHKHPTKIGAGAFVGSNSTLVAPVEIGANSYVGAGSVITEKVPEDSLALGRGRQIVK
                     ADWVSTRRKKR"
BASE COUNT         3047 a         4832 c         5033 g         3174 t
ORIGIN      
        1 gcctgcgggc gcaggcaacg tctacgccga gccggcggca gtgaatttcc agtccaatga
       61 tcgctatccg caaatggtgt tcgctttcct cgataccagc acccgggatg gattgggtgg
      121 ccgattcgcg gtgccgaaaa attacgccgg cagccccaag atcgttgtag actggtccac
      181 cacagcaaca gcgggaaatg ctgtgtggga tttcgactac acggcggtgg gtggcgatgc
      241 ggcagagagt tatgacccct cggctgatca gcaatcggtg acggtgaccg atgccgcttc
      301 cgccacggcc aggcgccggc agagctgcga gatgtctctt accgcgagca atcttgcgcc
      361 tgatgacacc gtgcagttta ccctcttccg cgacggggca ggtggtgaca ccatcgcggc
      421 cacgctctat ctattcggct tgtacttcga attctctgac gtctgatggc cctccagttc
      481 cagtcgacgg cggccaaggt caatcacggc agtggtgcct ccatcgatgg cgccacagcc
      541 tggacggtgt gcatgtgggt acggccagcc gctgtatcgg gcaatggcca gttcatgcac
      601 aagcagcagg acgggctcgg ccactcgctg tatcgcgatg gtgcccagat cggcggctat
      661 accagttgtg ccggctatag ctatgatgag cggatcagtg aagacgtgct cacggcgggg
      721 caatggagtt tcgttgcgtg gacgtattct ttcgctgaca agcacaggat ctatcacggc
      781 acattgggat ccgcgccggc ggagacgcca tactcgatca ctgacaactc cgggagcgga
      841 gctccggctg atgattcggc gtacccgctg gaggtgggca actatcagga ctcgtacccg
      901 tttggtggat cgatcgccgg cttccagtac ttccgccgcg tcctgactcc cgccgaaatc
      961 aggctggtgt ggatgcggcc ggctaacgcc atgctatttc agcccgcgct gtggtccgag
     1021 tatcacggca ccggtgcaca ggctgactgg tctggcaatg gcaatgccgg cacggtcagc
     1081 ggtggagcga cggtagcaaa tcatgtgccg ctgggccctc cattcgggtg ggatgacgcc
     1141 acgacctggg ccgtcgtctc aaccggtaca acacatgccc tcgcagggta tgttgccggt
     1201 tggtccagtt cgactgcgac gctgaaagcg agactgcatc tcgccggcag agctgcgggc
     1261 caatcctctc aggtcggggt ctttcgggca cgaaaggtgg cacgtgccac ggcagcggga
     1321 aattcctcga gctctgctgt cattcgtgcg attcgtggtg cgcgggcgac agcagccggg
     1381 gcatcggtcg ggcgcgggtt ggttcgcctg gtgcgcgcag ctcgcggtgt cgcatccggt
     1441 gcttcgtcgg cggctgcggt atggcgtatt cgcagtgcgt tgcgaggcgc cgcctccgga
     1501 gtatcctggg ccgtagccat cgtgcatggc gcggccgggg ccatcatctc gctcttcggc
     1561 cggacacagg gcgcatcgtc ctctgtgggc acgctgcgcg ttgccaggcg caccagagca
     1621 accgccgccg gcgcggggtc tgccgatgca atgctcagga tcgccaggaa gctggccggg
     1681 aagacggtcg gcagtgcttt cgggcaaggg gtcttacgcc gcatgcgggc cgtagggggc
     1741 attgcgcaag gatcctcgac ggctcatggc aaactcgctc tgatccggcg gctgattggc
     1801 gcggccgcgg gatctggcga gacagcaggg cgcatggccg gccgccgcgt gcttcgcgcc
     1861 gtggcagcag gaaaggcggg gctcaggggt gtactcacac tcatcaaggc caatttccgc
     1921 gtcagtgccg agcgcacgat cgttgtcccc tacgaggacc ggattatcgc ctgcgaggcc
     1981 gagcagcgtc tgatgacgcc accggccgag aaccggatca ttcaggtttg aggaaatgaa
     2041 aacgttcatc aagcacgcca atgccgtcaa ggactacggc ttcgacgtca ctaaatggct
     2101 ggctggagac acgattgaaa gccgcacctg gtcggtgccg gcaggcctga cgaaagggca
     2161 ggagatcgcg gacggccccg tgtccgtcgt ctttctgggc ggcggcgaag tggacaagac
     2221 cgatctgtcg acttaccttg tgtcgctgac tatcaccact gctggcgggc gcaccgaagt
     2281 gttcccgttc cggctacgga tcatatgacc gacaacctga gaacgatcga ccggctgctg
     2341 tactggctgg gcgtatacgt cgtgatgctg ttcgtgctgg cgtgggccgc cggcaaaccg
     2401 ggcgaggcaa ttcctgtact gacgaatctg atttcgggtt tcgccggagc agcattcgcg
     2461 atcatgcgtc tcggcaaaga acagcggttc gagcggttca gcaaggtcga caaagaccag
     2521 aaataaccct ggatccggct ccagccgcgt tgctttccac cggggatatt ccgcacttgc
     2581 ggcaattggt caatacagcg agttgtcacg tcgtcatctt ggctgtaagt cattgatttc
     2641 ccgttcgaca ggcaggctga attggtcaga atggctaaac tgaccaattc gctaccgccg
     2701 cattttccca atgtccatag atctctcttc cctcggcccc gccgatctcg aggcgcttca
     2761 cggggtgttg gcaaaactga aaggcggcgc cgataattcg gacgtgaaga agcgtcgcaa
     2821 gcgccccaag aagcaagcca tacagtacct ggaggaagac gaaatcgaac ggctcttccg
     2881 ggccgtgaag gacccgcgcg acaaggccat cctgcgggtg gcctaccacc gcggactacg
     2941 ggccagcgag atcggccgcc tcgatctgtc cgactaccgt gtgaaggacg accggctcca
     3001 ggtgaaccgg ctgaagggct ccgcctcggg cgaataccac ctctgtgcga acgaggtgcg
     3061 tgcgctcaag gcctggctca agatccgggg cccggcgcca ggtcccctat tccccgcgcg
     3121 tacgggcaaa ggcatcagcc ggcaaatgct cgacgtgctg atccggcgat acggcgccgc
     3181 ggccgggctg ccgtgcgaga agtgtcatat gcacgccctc aagcactcct gtggtaccca
     3241 cctcctgaac cgtggcgagt cgatcgagga tgtgcaggat cacctcggac atcgcaacat
     3301 ccagaacacg ctggtgtacg ccaagctcac gaatcgccgg cgcgagcagc gggataagcg
     3361 cctgcgggac tggtaacacc caaggccttc atcacggact cgggctcccg gtcaatgacc
     3421 gtgagataag cccgcaccgc gctgtccggc ttcgcacggc cctgctccca ctcctggagc
     3481 gtgcggaggc taaacccgaa ccgctccgcg aattcggcct gggacagacc cgtcctggcg
     3541 cggacctccc gcacatctct ctcccgtttc ggtcctctgc cctttgtcat gccgcatcca
     3601 gcccttccgc gatcagctta gccgcgagcg cgctcgaatc cgtgtgttcc tccctggcgc
     3661 gctcgtccag gcgggtgcgc aatttggccg gtacgggcat cacggagcgt tcccccggca
     3721 gcggaattgg atcgccgaat tcctgcttcg tcagcaacac tgaccggagt gcgtccttcc
     3781 cgttccgaat tgcctcttcg ggacttgcgc catcggaaat gcagcccggg atgtccgggt
     3841 actcaatcag gtagccgctg ccttcgtccg ccgtcatcgg gcggacgatg aatggatact
     3901 catcaattct ggattctaga ccgctcattc tgctttctcc atgctgtcga ccaacttcag
     3961 gaacagccgt acgtacacag gctggatagg ccgcgccgcc ggaaccggga ggacctcgcc
     4021 gttgggagcc cggaacgtga cgtgactggt tcccttctgc cgatatgcga tgccgaggtg
     4081 atcggcaatg gctttcaggt cctcgatcct ccagtccctc ggattgttcc gcatcttctg
     4141 gagggttttc atcgcgctcg gcatgattta actatacgtg attcacgtag tattgtcaat
     4201 ggttatccaa gggcaaacag gtcggagttg tactagaacc ttccgttgcc gcagttaaag
     4261 gcctgcggcc gatccgtcga tggcttattg gatgtcaagg gaaccgctgg cggcaacctg
     4321 gaagaacaag ctcttcttcg gtgacaacct cgtcatcctt cacgagcaga tcccggatga
     4381 atccgtcgac ctggtctacc tggacccgcc gttcaattcc aacgccacct ataacgtgct
     4441 gtttgccgaa cgcagcggcg ccaagtcgag cgcgcagatc cgcgcgttcg acgatacctg
     4501 gcagtggtcg atggagtccg agttggccta tcacgagacg gtaactcgag gcggcaagct
     4561 ggcggacctg ttgcaggcat tgcgcgcgtt ccttggccag agcgacatga tggcgtacct
     4621 ggtcatgatg gccccacggc tggcagagtt gcaccgggtg ctgaaacgga caggcagcct
     4681 gtatctgcac tgcgatccga cggcgagcca ttacctgaag ctgctgatgg atgcggtctt
     4741 cgggccggag aacttccgga acgagattgt gtggaagcga accagctctc acgggaacgt
     4801 ttcggctaat tatggcgacg tgatcgacac gctcctctat tacagccgtg aaccgaagcc
     4861 catctggaat cagctttacg tcccatactc ggaagaccac attcgaaata agttcaccta
     4921 ccgagacgaa cacggaaggg ccttcaccac gagtgatctg cgaaacccga gtctccggcc
     4981 aaatctgatt tacgaataca agggctacaa acctcacccg aatggttggt ctataagcag
     5041 gcaaaagatg gagcaatacg atcgcgaagg aaggctgtgg tttccctccg atccagatgg
     5101 ccgtatccgt ctgaagcgct accttgatga acagccaggg cagaaagtgc agaatctgtg
     5161 ggacgacgtc aagcccatca actctcaggc acaggagcgg ctcggctacc cgacgcaaaa
     5221 gccggaagct ctgctcgaac gcatcctccg cgccagctcc agggagggcg atgtcttcct
     5281 ggatccgttt tgcggttgcg gaaccgcagt gcatgtggcc gaaaagctca ggcgccggtg
     5341 gatcggaatc gacatcacgc acctggccat caccctgatc cggcatcgcc tgcacaatgc
     5401 cttcggcagg gacctggagc cgtatgaagt gatcggagat ccaaaggacg tcgagagtgc
     5461 gcgcactctg gccgtgctcg accgctatca gttcgaatgg tgggcgctgg gcctggtgga
     5521 cggcaggcca gcgcacgaca agaaaaaggg ggccgaccaa ggcattgacg gactgatcta
     5581 tttcttcgac gacgggacca acgtcgcgaa gaaagccgtg gtccaggtga agagcgggca
     5641 cgtgacgagg aaccagatcg gggacctcaa tcacgcgagg caaagggaga aggcggaagt
     5701 cggcgtgttt attacgcttg aggacgccac cgagcccatg cggagagagg cctgctcggc
     5761 cggcttctat cagccggact actttcccgg cctgaaggtg ccgcggatcc agattctgac
     5821 gatcgcggaa ctgctggcgg gcaagaaggt cgagttgccc atggcggccg tgccggcgac
     5881 gttcaggaaa gcggcccggg cgcacaagaa cgcggagccg caacaaacca gcattttttg
     5941 aagcggtatt cctcgggccg ccgccgtcgg cttgctaggc ggcatcttcg acaccgacga
     6001 caatccgctt gccgaggacc tcaagtgcgg cctgcaactt ttcggttttg gtctcgtgct
     6061 cagggtccag catgcgccgc acaaccgttt cgcgcacgcc caggcgccgg gccaactccg
     6121 aattgtttat gcccttctcc cgcactgcaa gatagaggcc taacttgccg gcgatccaga
     6181 ggggaaaggt ggaagaaatg gctgctgtag ccgctttcga cagttcggct tatcgtaagc
     6241 tgctggcccg tacgcttccg aaggccatca aaactctgga agagaacgag cgcatgaccg
     6301 cggaactgga acggctcgac aatctggacc gtccgctgac ggccgaggag aagacgttcg
     6361 ctgagcttgt gaccgtcctg atccagcagt tcgagacttc gcgctatccc ctgggccacg
     6421 ccgagccggt ggaagcgctg cgggtcatga tggaacagca taatctgcgc cagcgcgacc
     6481 tggtgccggt attcggatcg agcagcgtcg tctcggacgt gctgaacggc aagcgcggca
     6541 tcagcaagtc gcacgcgaaa aagctggccg agttctttca cgttcctgcc gacctgttca
     6601 tctgagcgcc ttagtacttg cctccattga cgcgcggata cgcgtctttt aggcttgagt
     6661 ttgtccgcag ttttgcggcg cggcggcgcc gcggtcttgc ggcgaaccct atgcctgact
     6721 ccaaacctaa atctgaaaca cccagcgaag tgtggctacc gcgcgacaag gcactggcca
     6781 acctggccag gtcgatcgac gtactgcaac ggcatgtgaa ggcgggccac atccgcaagc
     6841 gcgggctggg cgccacagcg gtctacaacg cgcaggacat cctggcggtg aggaccggtg
     6901 cgcacaagat gcacatcgag agcggcgggc aggccgcagc ggcggtgccc ttgcaggcgc
     6961 aggccgatgc ctggagccat ctcgccgggt tcctcgccaa cctgaccaag acggtcgcag
     7021 agatcaacca gcaggcggcg ttgcctgctc cggagcccgc ggccgaggat gtgccgcctt
     7081 tcggttacct ctctgtggct gaagccgcgc ggctggtgcg gatgccgcgg tcctggatcc
     7141 tggagcagat cgagagcggg cggttagcct acgcggtgac ctcgcgcggc ggctaccgcg
     7201 tgcggcgggc ggatctcgaa gcgatgccgg cgccggagcg cgtccgaaaa cacgccgcag
     7261 ggcgctgagc gcttgggcca cggcccattt acccgtatca tcagcgatat gggaaatcag
     7321 acatcggcgc gcaagcgcat cagctatgcc aacctcctgc ggcaggcctg cgccttcaga
     7381 cgcaacctcg cctcagctca tcccgccatt gccctgccgg cgaagctcaa gagcatccag
     7441 acacaagtgc aggcctatac ggactggctc gcgactttgc agaccaaggg attgttcgcc
     7501 gtgacgcccc cgaaacggcc cgcagcgaag ccgaaggcga tggcggcagg ggtaggggct
     7561 ccgacggcgg tcgcgggttc tacggggtcc cggcggcgcg gagcggggcg cgccagcaag
     7621 gcgcgacggg cacaagcctg agttcgagga atggaagcac gcaggcgcat gaaggcgatt
     7681 gcgcggcggg gaccgcaggt cactccagca tgccgttgaa aagtcccggc tgggaatcct
     7741 gacttttttc cgatttccgc cgtttccgcc ggacaggcgg cgccaggatg gtgtccgttg
     7801 gtttcggcac gatgtgtact ggctggtcgg tgttgcgggc gttctcgtgc gaatccggaa
     7861 acagaagctt gtaggcgtta tccagatcgg gccagaactt ctcgaatgtt ctctcgagca
     7921 cgcgatcctt tatcggccga tcggagagtt tgttgaagga tttgacgaga tggaacgcgc
     7981 tctggctatt gatgcggagg ccgttgaact gattcagggg ttgttgcagt tcgggatagt
     8041 cgatatcgag caggattgtg gccactcggc tctttcccag gcccttgacc atacccccgg
     8101 cttcgaaatg aatccaaggg gcgaggtggt tctcacgcgt gatgcaaatg acgccgcagt
     8161 tgcaccgttc cagttcacca ataatttcgg tgaaccagat ggtgcctttg tcaatgtcct
     8221 cgctagatag ccagacgtcg gtaccgggca gcacctgttc aagccacggt ttgagttctt
     8281 cggcgaaagt gcggctctgt tcaccggacc aactgatgaa gattttcacg gccgggctta
     8341 cctgcggcac atgctatcag agtgcgctca ggggttgcta ctcgtgaggg cgcggggcta
     8401 ccacatcccg ccgtagcggt gcacctgatt tcgcagccgc tgcactaaca cctcgatctc
     8461 agacggctcg atcccgcgca gctcgcgcca ggcgcttcgc caatcgtctt tcgccgcctt
     8521 aatggcgtcg cgaaaagtac gccggataaa tacgagcacg cgacgcgggc gggccacctc
     8581 catacacagg cggcggtgct gctcctcggc ctcagaccag gtggagcatc gcgccatctc
     8641 ccactggtct tctccaagcc aaaatacagc cgtttcgaac aggaccggcg ggccatccag
     8701 ccggaacggt agtccgtgat ccagaccgag gaacacggtg ctcacctcgc agagcccgag
     8761 gatccgtgtg cgcatcacgg ttcggtcgtt ctcctcgaac catcgcgccc attcgagcag
     8821 gtcgtcgcag ggcactgggg tttggccgat gagggtgtag tagtggatca cttctgattc
     8881 gtgccctcga cgccccgttt gattcgctcc cgagtgcggc gttgcagcca cattagagcc
     8941 tctttgcagt gcgtcaatgc gatggcatta tcccggcatg agaatggtcc agcttgaaac
     9001 gaacgcaacc ggtctatcac gatggcgagc aaggcttcct gtgtgatgcc attgacgcca
     9061 aactctttaa tcgggccgtt ttggaacagc acgacttgac ggcagaagct cacgtcgggt
     9121 tccgtttgag agcgggatgg attggtcgtg gcatccatac ccgtgatttc gtatcggtga
     9181 ttcgctcctc cttgtcccgg ctcgtccgtg acggcaatct tcaactgcac ggcttgatcg
     9241 ccgctgacga catgatccgt aatcgttctc atctgttcct ttccttgaaa catcgtagtt
     9301 gtcgggcttt tggctatcgt tcatacgagg tccttcagcc gtttgccgat gagcgcccac
     9361 gcgacgcggg cgggccacct ccatacacag gcggcggctc agccgcctcg gccgcctcgg
     9421 tgatggcacg ggcgaaatcg cgcgcctgtt cgggcgacat ctggccgatg atctgatcgc
     9481 cgcgaagcag ttgcacgaac ggctcgccgc gcggactgac gccgcactgg tgccagagga
     9541 tatcgtagtt gtcgggttct ttgttagggt tcatttgagg tccttcagcc gtttgccgat
     9601 gagcgcctgg cagatttcgt ccacctgctg attgtgaaca tcgcactcga tcacgccgat
     9661 cgcccactcc aacatgttgc gcaagtcgcg cacatactcc ggcccgccgt ggcacttttc
     9721 gggctgggtt tttatcaccg tcagcgcctc gccgaatttc gcaagaagcc gcgtgcgggg
     9781 atccagactg aaatccaact cgtcgaaatg ccggcgggtc gccttgtgct gcggagcgtg
     9841 ctcgtcccag gtctggctca tgtgtcgtgc tccggattcc atccaagatc ggctatcgcc
     9901 gcgagcatcg gcgtggtgga tacctcctgc tggagcagtt cggcgaactg gcgcgcgatg
     9961 cgggcgccgg ggccggtgtt gccgggatgc cgcgcggcaa gctggagctg gccgagcacg
    10021 ctccacgcct cagtgtaggt cagcgccacg gtgatgcggc cactctggcg cttcaggtcc
    10081 tcgcgcgcca cgtcgaagag gtcaggccgg tcaggcatct ttcatcgtcc ggaactcctc
    10141 ggggatctcg atgggtgtcc agcgtgggtt cgtagcgttc aaatcattgg tgaatttgat
    10201 ccaatggcag aacaacgtct tgtctccttc ccgtgttgcg gcgccggtgg catagacgat
    10261 caggaacagg agctggcaga aattttcgcg cgtcatctcc aacgtgacgc ggtcgccctg
    10321 ctcggtgtag gtcactggat tggtcctcct accgccgctc gctgcggctg tagcgcgcgc
    10381 ctgagtttct cgcgcaactc cgcgggcagc agttcttcgt gaataaaggc accggacgga
    10441 cagacggctt cgccggcgac ggcgagattc agttccagct tttccgcggc ggtgaagtcc
    10501 gcgcggatcc gctcccaggt gtccatgcgt agcaggatca tgccgctcct cctctcacgt
    10561 gcacgcccgc gtccctgacg gtgcgcgctc cacagtgcgg acactgctcg ccgtcgagca
    10621 ggccgtacat ccagccgcga ttgcaggcgc cgcacaacca cggctttgtc gcctgccact
    10681 tggggaagta caggcattcg cggcgctcgt gcgtatgggg atcttcgacg atcatcattg
    10741 ttcctttttc gccaattttt gccgctccgc acacaggcgc tcatcgcggc agcggcgctt
    10801 gccgccgggc agccgcacat agtcgagcgt gctgaagaag cggcggccgc agagggcgca
    10861 ggggatctca gggcgtttca tcggattcct ccgcgtagag cgtctgctgg cgcgggtccg
    10921 gagggtgccg ccgcttacgg ccggtctgga cggcgatttc gtccagccag atctgatacg
    10981 ggtgattccg gcgggcgccg aagggatagg catcccgcag cgccgcgcgg atctcggctt
    11041 ccggccgccc ggcggtgtcg gcgagcacac ggcggatgat gggggccgcg tagctgcgcc
    11101 agcgggaggt catgacgaac ctccgccggc ggtcccggtc cgtgtgccct cgtccgcgac
    11161 gtcatagccg cgctttcgaa actccgctat gagtgtgata tcgtccatag cctccacgat
    11221 gctcatggcc gccacccaga ccgctcttgg ggtggcaagc cggacgggac acaccggcgg
    11281 cgagcagtcg cactcctcac aacccaggaa gtgctccaga gccagcagcg cgcccaccca
    11341 caactcatgc gcctgtatct gctccgggtc gccgaaccgg agcggcggcc gcaggacctg
    11401 ttgtgcctcg gcaatagtca tcaccggaaa tactctcctt tcatgaaccg gatgaacgcg
    11461 tcgccggcgc gtcgccaaaa tgcatcccag gcggcgcggt cctcgagcgc gcggttcacc
    11521 accttgcggc cctcggcggt ttgcatccag gtgaaggcgg ccgcagcggc gttggcgacg
    11581 gccgtgatcg ccgccaggac ctcggagggt gtcgggatca tgatgagcct ccgccggcgg
    11641 tcttggtttt gtttgcgtgg gccgctagcg tgtcgcgtgc ccactggtgt agtccgctgc
    11701 cagcttgggg atctgaaagg atatctctgg cttcggcgat gagtgcggcg atgtcttccg
    11761 ggctggatgg cgcaggtggt aatggcgcgg cgctggagcg ctggcggcgc cgctggatct
    11821 cctggtatgt attgccaatg tccccagcaa agtgctcgat cacgccgagc gatgtgataa
    11881 tccgccggcg gagccgtatc cgctgagtga gccactccac cggcgcgccg cgaagcttgg
    11941 cgtggatccg ctgaagcagc cgccgggagg gaacctcgcc caggtcttct atcagatcct
    12001 cggggatggc ttgccggatg gcttcgagtt cgggttcggg cggcagggtg tcagtcggtc
    12061 ggtctgctga ctgactgact gacactgccg tcgtcacggt cggagacgac gacgacggct
    12121 cctcaagccc gtcccttttc tttcatcctt attcgggggc gcctgcgcgc ggagaccttt
    12181 cgcgtaggcg cgcagcgaca attcggcctc cattgtcacg cgcgcaaggc gttccagctc
    12241 cagaatgtca ccgcgcgcaa tgacaaaatc ttccgaaaac cgtatccggt agtgcttcag
    12301 gaggggtctg aggtcggcgg ggcaccactc aaaattgtca ccgcgcgcgg tgacaatttt
    12361 tttcacgtcc acattacgag ggacggccca agcatatatc tccacacgcc cttttgtcga
    12421 gcctttgcag tcggcgaggc cgtagccttc caactctgtc atgtggcgcc ggaagtgttc
    12481 cctccggatt ccggtctccg cgcagatgtc ctgcggcgtc aatggtacac ttttgccgcc
    12541 ctccaccttt accgcgagct cctgccggaa ggccatggta tgcaggccga gacagatgtg
    12601 gatacgcgca gccggcgaga atgcctgact cttcatggcg cgtctggcgc gaggtttcga
    12661 gtctacgaag tactcgccct ctcggacacc caattgtgcg agccagcccg gcaccttgtt
    12721 gttggtacca gtactcattc ggagcctccg cggcgggatt tagttcggac tttacgtcca
    12781 atcaaaacat agccattcgg ggtctgaccg gagccggcat ggacctcgat gaagccacgt
    12841 tcggtgaggc tgtccactgc atcctccaac cggcggggag acaggccagt gtgttgcgag
    12901 agcgcctcga aactgcggcg catcaatgtg ttgcgggagt cgggcgattc gccgatgaaa
    12961 tacagcagca gtcgaacttc accctcctta agggttggcg cgatgcgctg gagccaggcc
    13021 aaaagacgat cagcggaggg catccatcac ccccacacat ggctcaatgg tgcttgcgtc
    13081 cacgcctgtg caaggtaaca ctggagatgc agaacggcac tccggtgtcg cctctgtatc
    13141 ctctggcgtg gctgcctgga tttcgcggcg ttcaatcttg gcggatttgg ccgcggggtt
    13201 caaaaacttc gtcagaggcg gactcttaag gtgcgtataa ttttgagtta tgctggccaa
    13261 ttgcgaacac agccgaacgg aggtaattgc caggcgggac ggcgtcgatt acgtacgatg
    13321 cctggggtgc ggacaggtgt ttgaggccga tgacctcgac ccggttccgg cgagcgaaga
    13381 ggacgatcaa gcgctctgaa atgtaaactt ctgcgcgggt cagtttaaac ggggtactgg
    13441 agcggattcg gtcagtgtcc tgagaccggg agatattctc agcccggcat ccggacgttt
    13501 acctcgccca gatgtgcgaa catagacttg tactggcggc tcgcagcata gcgcgtgctg
    13561 aatggagcta gttaagagcg cacaaataag gcatctgacg attgaccggc tcctctgtta
    13621 attcgtctgt ccagcgtacc cgcaacgccc tgaagatcgt tgccatcggt ggcggcacgg
    13681 gtttatccgc actcctgcac ggtttgaaac cgcatgtgtt tgaatcccct aacggttcac
    13741 cctacgttcg gaggccccgg ctcgaaatta cggcgattgt aacagtgacc gacgacggag
    13801 gcagctccgg ccggctccgg cgccaactgg atgtgctgcc gcctggggat atccgcaatt
    13861 gtatggtggc gctttcagag gatgaggcac tgatgtcccg gctgttccag taccgctttt
    13921 cgagcggaca cggcctgcgc gggcacagct tcggaaacct ttttctcacg gcgcttaccc
    13981 atttgaccgg cgattttccg ttggccatca aactttcctc cgacattctt gcgagcgtcg
    14041 gccgtattta tccctcgacg gccgagaatg tagtccttcg ggccacgctc gaggacgggt
    14101 ctaccgtgct gggtgagaca cagatcagcc gcagtacctc acggattgag cggatttcgc
    14161 tcgagccacg caggtgtaaa ccactcaagg aggcccttcg cgccatcgag gaagcggacc
    14221 ttatcacgct gggtccggga tctttgttca ccagcgttgt cccgaatctg ctggtgcagg
    14281 gaatccccag tgccatccag cactccccgg cgatcaaggc gcacatcgcg aacctgatgt
    14341 ggcagccggg cgagaccatg gggttcaccg tggccgacca tgtggacgtc atccatcgcc
    14401 atgcgcggcg taaaatactg gattgcgtag tggtcaacac ccatcctatt gccgaacagc
    14461 agaggaggcg ttatgcgaga gcccgggtga aatccgtgga agtcgaaccg gatcgcttga
    14521 atgcgatggg cgtctccgta atcggaaggg acttgctcgc tcccgtgtcc ggtaaagctg
    14581 ccattgtgcg ccacgaccct gccgcgaccg ccgctgttgc catcgagctt gcggccgccg
    14641 ggcggcgcag gcggaatagg ctggcaccgc aacaaaaaca cctggttggc tgaatatacg
    14701 acatgttgac ggatttgaga gtggttgtac tcgccgccgg actcggcacg cgaatgaaat
    14761 cgaagagggc caaggtcctg catcgggccg gaggccgcac gttgatcgat catgtcgtgg
    14821 gaacggcact cgaactgacc acccccgaac aggtggccgt ggtggtcggc catcagcgcg
    14881 aatatgtaga agaactgatg gcaccccggc gggttcgctt cgtacaccag caggagcaga
    14941 aaggaaccgg gcacgccgtc atggtgtgcc gcgatacgct agaaagccac accggtctgc
    15001 tgctggttct ttacggtgat tgcccgctct tgtcggtgga aacattgcgt gcgctgattg
    15061 aacggcaacg ccagtcgtcc gccgcggcga ctgtcatcac caccactctg gaagacccga
    15121 cgggttacgg gcgggtgctt cttgacgccg ggggcagtgt acaggccatc gtcgaacaga
    15181 aagcggcaac gccggaggag cttcaggtgc gtgtaatcaa ctccggtatc tactgttttc
    15241 gctccgatct gttgtgggaa tcgatctctt ccatccagcc ggacaacccg gcgcgcgagt
    15301 attatctcac cgacattgtg aagatcttcc acgcctcggg ccacaaggtt aacgccatgc
    15361 atctcgacga tgcgagcgag gtgcttggca tcaacacgag aatcgaactc gcagaggttg
    15421 acagaatttt tcgggagcgg aaggtcaggc agttgatgct ggatggcgtc actatcgaaa
    15481 aaccggagac cgtcactgtc gatttggatg ccacagtagg accggacacc gttatcgaag
    15541 ccttcgcgcg tattctgggc ggtactcaaa tcggagaaga ttgcacggtc ggagcatact
    15601 ccatcgtgtg cgattcccac atagcggacg gcgtgacaat tcatccattt tcggtagtgg
    15661 agttgtccac catcagcggc ggcgctcaca tcgggcccta ctcgcggtta cgcatgggta
    15721 gccatgtgga ggctggagcc cacatcggaa atttcgtgga gctgaaaaag acccgcatcg
    15781 ggccgggcag taaggcgaac catctcgctt accttggaga ttccgagatc ggccctggcg
    15841 tgaatatcgg cgctggtacg atcacatgca actacgacgg ggttcataag cacccgacca
    15901 aaatcggtgc aggcgcattc gtgggcagca actccacact ggttgcaccg gtagagattg
    15961 gggccaacag ttatgtcggc gcaggttctg tgatcacaga gaaggttccc gaagattcgc
    16021 tggcattggg acgtggccgt cagatcgtga aagcggactg ggtgagtacg cggcggaaga
    16081 agcggg
//
LOCUS       DASTMK010000597         9895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_286752, whole genome shotgun sequence.
ACCESSION   DASTMK010000597 DASTMK010000000
VERSION     DASTMK010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286752"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFQ79_24660"
     CDS             <1..231
                     /locus_tag="VFQ79_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HEU0142935.1"
                     /translation="ARVLVLAGALMGGEIEKAVREVRDRGLLVISLNMAGSVPEAADL
                     VVTDPVQAGVMAVMATADTAKFNIERLKRRVF"
     gene            complement(329..967)
                     /locus_tag="VFQ79_24665"
     CDS             complement(329..967)
                     /locus_tag="VFQ79_24665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142936.1"
                     /translation="MMMVDDMTGSHASDELLEQYAMSKLLEPEAADLEEHLLICSECR
                     DRLEETETFLIAFRQAVQDPAPARRSLLNRIFANLSLPQVPKLAWVPVAAAIALLAIW
                     IPTQQESGTSVKSVELMAYRSGDGNGPAAIRSGERVELRADLTGVPAAEAYRVEIVDR
                     EGDVVYRSEAKPDANKLVVSIKDGLEAGRYWVRIYQPDQTEAPLREFGIQVE"
     gene            complement(1006..1701)
                     /locus_tag="VFQ79_24670"
     CDS             complement(1006..1701)
                     /locus_tag="VFQ79_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0142937.1"
                     /translation="MAPIACQPDLLVDPKESDVQENDLPPSPAGPTPEAAQDWTCLVE
                     RIQAGEEDAMEELYQLFSKGIRFFLCRSLGSQELDDKVHDAFLIVVKAIRRGELRDPQ
                     RLMGFVRTIVRRQVAGHIDRAVHARREQVDIESGVRLVDPSLNPEQRIVAKEKADLMK
                     TVLSELATRDREILTRFYLLEQSQEQICADMELTETQFRLLKSRAKGRFGDLGKKKQV
                     RRQLSVFLRASSR"
     gene            complement(1707..3968)
                     /locus_tag="VFQ79_24675"
     CDS             complement(1707..3968)
                     /locus_tag="VFQ79_24675"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEU0142938.1"
                     /translation="MALEKGDTASAIRVLNNLGAAHLYLLQYRRAMQVYIRAKDLASR
                     AGDAESGPAISGNIASIYLQMGDEKGAWEEVQQALDGLAPETSNAVRARLFILAGGIQ
                     SYLGDLAAATRYFGAAIATADAEADPAMEALAWDHLGEAWQRAGDLDRAEIALTNAFR
                     LRKLNGGKDLFLSYPKLGLLRLAQGDLHSAEVLMNRAIETSRQASAQMPLWTPYHRRG
                     LVKKAGGKLREALGDFQTAIDMVRVARLEVIPVASLKNSSNVELHDVYSSFIETACAL
                     YLETRVRRYAEMGLQAAQEIRALALRDRVGDLESRLPAEYWERLAQLRAAQAAAFRNR
                     TAAVDDRIDRLNVELNEMEARAGLEAHVVHPSAPGAVHDFGLGRDELLLSFHITPNVS
                     VVWALTDRDLELRPLPPEKVIARQVAGFQHAVQTGSTTAATQGVELYRTLFDDLPEQA
                     LARKRWKILLDGPLYKLPFAALVDSFEDEKPRYLVENHVLLVMPAILKTPGTDVRDSG
                     LFVAAGDPIYNQADSRWQGPRTPRSARKTGSLFSLFGRAVDAEGSRSFELARLPGTGR
                     EIRACANVWAAKSGVKPELLAGAEANWDSIRATFARKPAVVHIASHVVSSPVNPEEAM
                     IALSLGPDGSGDFVGPLQISALRFQTGIVVMSGCNSGTGEALPGAGLMGLTRAWLHGG
                     AGAVAATHWPVTDDEGKLLVRFYEHLHQLKGGSAGARAAEALHLAQRDFLKEETGQTH
                     ARDWPAYFVVGKE"
     gene            complement(4555..5649)
                     /locus_tag="VFQ79_24680"
     CDS             complement(4555..5649)
                     /locus_tag="VFQ79_24680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0142939.1"
                     /translation="MNRRTALGPVSRRAVLSAAAAAIASAQQPRSEVRLPRKVRIAII
                     GFDGHPGEITGVLSRLPDVEVAAISDKPAAMARAARNPRLAGAKQFTDYERMLDGEKV
                     DVVAVCNNNGERAGAVIASAKRGLNVIAEKPLAIVTSDLERVKQAVSASGIQLGMLLP
                     MRYSPSYLALKQIVDSGELGEIALISAQKSYKAGSRPEWMLRSETYGGTIPWIGIHMV
                     DLMRWTSGREFTATASFQGIVGSPGNKAMENTTASIFQLDNGGTATLRMDYFRPESAP
                     THGDDRLRLAGTKGVAEYMAATGVTVMTDKAKPRVISDLPPGGSVFIDYLLSVYAGKE
                     PTLTLKDIYRTNEIVLAARDAADQRTILKL"
     gene            complement(5691..5987)
                     /locus_tag="VFQ79_24685"
     CDS             complement(5691..5987)
                     /locus_tag="VFQ79_24685"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEU0142940.1"
                     /translation="MLIARVIGEVVATRKHASHEGRKILMVQPLNLDGTDRGDAVVAL
                     DSVDAGVGDRVLLVTEGFSAMTSVGRPQSPIDMAVIGVIDCVDLLPEAAQAKTP"
     gene            complement(5992..6276)
                     /locus_tag="VFQ79_24690"
     CDS             complement(5992..6276)
                     /locus_tag="VFQ79_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEU0142941.1"
                     /translation="MYLGKVVGKVWATVKNANLDGHRLLIVQPLTPELKPTGRRIVVA
                     DCTGAGAGELVYWCRGRESTFPFLPDEVPADSTIVGIVDELTIKRTKEAG"
     gene            complement(6286..6552)
                     /locus_tag="VFQ79_24695"
     CDS             complement(6286..6552)
                     /locus_tag="VFQ79_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEU0142942.1"
                     /translation="MILARVVGTVVATRKDPRLEGKKLLVLKPISPEGRDETGYVVAV
                     DTVSAGFRETVIVVSGSSARMAEGCKDKPVDTAIVGIVDTISLD"
     gene            complement(6613..6900)
                     /gene="eutM"
                     /locus_tag="VFQ79_24700"
     CDS             complement(6613..6900)
                     /gene="eutM"
                     /locus_tag="VFQ79_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HEU0142943.1"
                     /translation="MGEALGMIETRGLVAMIEAADAMVKAAKVTLVGWEKIGSGYVTA
                     LVRGDVAAVRAATDAGAAAARRVGELISVHVIPRPHPSLEDVLPIGKSNSK"
     gene            complement(6937..7710)
                     /locus_tag="VFQ79_24705"
     CDS             complement(6937..7710)
                     /locus_tag="VFQ79_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0142944.1"
                     /translation="MPKTEREYRQDIVDVGRLVYQKGWVAANDGNISIRLDQERILCT
                     PTGVSKGMMHPDDLVICDMSGNKLEGRKERTSEMAMHLTIYGMRPDIKAVVHAHPPVA
                     TGFATAGKPLNLALLPEVIIGLGCVPLADYGLPGTPALTDPMLPYIPKYDAILMANHG
                     AVCYGEDVYKAFFKMETTEHYARISLVAELLGGATILPKQEVEKLFDSRTRYGVKARA
                     GVEPGCPVAAEDADAGKFVVTRDELVALVDEALRMRGVA"
     gene            7876..8382
                     /locus_tag="VFQ79_24710"
     CDS             7876..8382
                     /locus_tag="VFQ79_24710"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEU0142945.1"
                     /translation="MSIGKKWMTFATAGIVSAGLLLAQGPSTSRRGGPGGDPGARKER
                     MKGFMAEYLNLTESQKQQAEQIFQAARDSAKPLREQMKTGHTQMTDAIKTGKSDQELQ
                     AIADAQGALMGQVSGIHAKATAKFYAILDPAQKEKAEKLHNRFMGGMFGAGPGAARGG
                     PGMGRSRQ"
     gene            8530..9456
                     /locus_tag="VFQ79_24715"
     CDS             8530..9456
                     /locus_tag="VFQ79_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0142946.1"
                     /translation="MDLDQLHTFLEIVRLKSFSKAAQTCYRTQPAISAQVRQLEQELN
                     ASLFERLGTRIALTAAGKIFAEYAAQILDLRRQAQDAINELERVPRGELVIAANEATC
                     IYVLPVVFSEYKKQFPNVQLHVDRSYGSRVVSAVLENQADFGITQLPVQEKKLQVVKI
                     HSDEIRVLVPPGHPLARRTSVVAQDVIGCPLLLPKSGNTRARLNAWLELVEDHIQISM
                     ELDSTEMIKRFVMAGLGISFVAASNCREETASGRLATVALAPEPMMRRLGLIYRKDKA
                     LSKAALGFIQATLEYSASGELAVPNLKLASTL"
     gene            9453..9800
                     /locus_tag="VFQ79_24720"
     CDS             9453..9800
                     /locus_tag="VFQ79_24720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142947.1"
                     /translation="MSGSTFKHSTILIAAGKRTEVYRSLDQVPPALLRKLKECTGGSN
                     AGTILIADERGREEILRSLRDGAGAGTPVRDRQRVPHFDSLSSGRASWIRVLEVFIPT
                     ALAVLALLILMAE"
BASE COUNT         2017 a         2953 c         2869 g         2056 t
ORIGIN      
        1 gcgcgggtgc tggttctggc gggagcgttg atgggcggcg agatcgaaaa agcggtcagg
       61 gaagtacgcg accgggggtt gctggtgatc agcctgaaca tggcgggaag cgttccggag
      121 gcggcagacc tggtggtaac cgatccggtt caggcaggag tgatggcggt gatggctacg
      181 gccgacaccg ccaagttcaa catcgagcgg ctcaaaagaa gagtcttcta aagcttgttg
      241 aaaacgtaga aatccgcttg ctaacgcgcg cggctcagtc ccagagccgt gaccgtgagg
      301 gagcggtggc tcccagactt cctgaaaact actccacctg gattccgaat tcccgaagag
      361 gcgcttcggt ctgatccggt tgatagattc gtacccaata ccggcctgct tccagaccat
      421 ccttgataga gaccacgagc ttgttggcgt ccggtttcgc ttcggaccgg tagaccacat
      481 ctccttcccg gtcgacgatt tccacacggt aagcttcagc agccggtacg cctgtcaggt
      541 ctgcccggag ttctacgcgc tcgccggagc gtatcgcggc aggtccgttg ccatcaccgc
      601 tacgataggc catcagctca acacttttta cgctggtgcc gctttcctgt tgcgtgggaa
      661 tccatatcgc gagcagcgcg atggccgcgg ccactggcac ccaggccagt ttcggtacct
      721 ggggcaagga gaggttagcg aagatacggt tcagaaggct gcgccgggcg ggcgcgggat
      781 cctgtacagc ctgccggaat gcgatgagga aggtctcagt ttcttcgagc ctgtcccggc
      841 attccgaaca gatcagcaga tgctcttcga gatcggctgc ttcgggctcg agcaatttgc
      901 tcatcgcata ctgttcgagc aattcatcgc tcgcgtgaga acctgtcata tcgtccacca
      961 tcatcataaa tgaatgatgt cctccatctg ttgtatggag tgcggttaac gtgagctagc
     1021 tctcagaaaa accgaaagct ggcgccggac ctgtttcttt ttgccgaggt cgccaaatcg
     1081 tcccttcgcc cgtgatttca acaacctgaa ctgtgtttcc gtaagctcca tatccgcgca
     1141 gatttgttcc tggctttgct ccagaagata aaagcgggta agaatttcgc gatctcgtgt
     1201 cgccagttcg ctcaggacgg ttttcatgag atcggccttt tcctttgcca cgatgcgctg
     1261 ctccggattt aaggacggat ccacaagccg gacgccactt tcgatgtcca cttgttcccg
     1321 ccgggcgtgc accgcccggt cgatgtggcc ggccacctgg cgccgcacga tggtgcgcac
     1381 gaaacccatc aagcgttggg ggtccctcaa ctctccgcga cggatggctt ttacgacgat
     1441 taaaaacgcg tcgtggactt tatcgtccag ctcctgcgaa cctaacgaac ggcaaaggaa
     1501 aaagcggatg cctttggaga acaactgata aagctcttcc attgcatctt cttcccccgc
     1561 ctggatgcgt tcgacaagac atgtccagtc ctgcgccgct tccggggttg ggcctgcggg
     1621 ggagggcggc aaatcgtttt cttgcacgtc actctcctta ggatcaacga gaagatccgg
     1681 ctggcatgcg atgggcgcca taaattctat tcctttccaa ctacaaaata cgccggccaa
     1741 tctcgtgcgt gcgtctggcc agtctcttct ttcaaaaaat ctctctgtgc cagatgcagg
     1801 gcttcggcgg cacgagcgcc cgccgaccct cccttcaact ggtgtaagtg ctcataaaac
     1861 cgcaccagta gcttgccttc gtcgtccgta accggccagt gggtcgccgc gaccgctccg
     1921 gcgccaccat gcagccatgc cctggtcaga cccatcaggc ccgcgccggg caaagcctca
     1981 cccgttccgg aattgcagcc gctcatcacg acaattccgg tttggaaacg aagcgcggaa
     2041 atctggagcg gtcctacgaa atcacctgag ccatcgggcc cgagcgacaa tgcaatcatc
     2101 gcctcttcgg ggttcaccgg agagcttact acgtgggagg cgatgtggac tacagccggt
     2161 ttccgggcga aggtggcacg aatggaatcc caatttgcct ctgcgcccgc cagcagttcc
     2221 ggcttgacgc cgctttttgc ggcccagaca ttggcgcagg cccggatctc gcggccggtt
     2281 cccggtagac gagccagctc aaaagaccgg ctcccctccg catcgacagc ccggccgaaa
     2341 agagagaaca gggatccggt tttgcgggcg gagcgcggtg tccgcggccc ctgccagcgc
     2401 gagtcggcct ggttgtagat tggatcgccg gcggcgacaa agagtccact gtcacgcacg
     2461 tccgtgccgg gtgttttcag gatcgccggc atgacaagca gtacatgatt ttcgaccagg
     2521 tacctaggct tttcgtcctc gaaagaatcc acgagcgcgg caaatggcaa tttgtaaagc
     2581 ggcccatcca gcaaaatctt ccaacgtttc cgcgcgagtg cctgttccgg caagtcgtcg
     2641 aagagcgtcc ggtagagttc aactccctgt gtagcggcgg tggtggaacc cgtctggacg
     2701 gcgtgttgga agccggccac ctggcgggca atgacctttt ccggaggcaa cggccgcaac
     2761 tccaaatcac gatccgtcag agcccacacg acggacacgt ttggtgtgat gtggaagctc
     2821 aacagtaatt cgtcccggcc aagcccgaaa tcgtgcaccg cccccggagc ggagggatgt
     2881 actacgtgcg cttccaaacc cgcgcgcgct tccatttcat tgagctcgac gtttagccgg
     2941 tcgattctgt catccaccgc ggcggtcctg ttacggaacg ctgcggcctg tgcggcgcga
     3001 agctgggcga gccgttccca atattcggcg ggcaagcggg attcgaggtc cccaacacga
     3061 tcgcgaagcg ccagcgcgcg aatctcctgg gcagcttgga gacccatttc cgcgtatcgc
     3121 ctgacgcggg tttccagata cagcgcacag gcggtctcga taaaagacga gtagacatcg
     3181 tgtagttcga cgttgctgct gttcttcaaa gacgcaaccg gaatcacttc caggcgcgcc
     3241 acacggacca tatcgatagc cgtttgaaaa tcgccgagcg cttcgcgaag cttccccccg
     3301 gcttttttca ccaacccgcg cctgtggtac ggcgtccaga gcggcatttg agcgctcgcc
     3361 tgccgcgacg tttcaatggc gcgattcatg agaacttcgg ctgagtgcag gtcgccctgt
     3421 gcgagacgca gtaatcccaa tttgggatag gaaagaaaca ggtccttgcc gccattcaac
     3481 ttccgtagcc ggaaagcgtt cgtcagcgcg atttcggcgc ggtccaggtc tcccgcccgt
     3541 tgccatgcct cgccgagatg gtcccaggcc agcgcctcca ttgccggatc ggcttcggca
     3601 tcggccgtcg cgatcgccgc tccaaaatac cgggtagcgg cggcgagatc gcccaggtag
     3661 ctctggattc caccagccaa aataaacagt cgagccctga ccgcattcga tgtctccggg
     3721 gccaggccgt cgagcgcttg ctggacctct tcccaagcac ctttttcatc tcccatttgg
     3781 agatatatcg acgcgatgtt tcccgaaatg gccgggccac tctccgcatc gcctgctctg
     3841 gatgcaaggt cctttgcccg gatatacacc tgcattgcac gccgatattg cagcaaatac
     3901 agatgtgcgg cacccaaatt gttgagaaca cgaatggctg atgcagtatc gcctttttca
     3961 agagccatgt taaaaccctg ctcgtaaagc gctgccgcct ggagaaaacg gccttttccg
     4021 aggctgtcaa acgctttctg gcggagttca cgaaaggcgg catcattcca gagaaatgct
     4081 tgggaggagt tggaagagag tgaacggacc ggtcccggcc ggctgctcac atttggtagg
     4141 agcagcagca ggaaacccgt cgctgcggcg gtaaatcgca cgagatgaac aagagagaga
     4201 aaaggaacgg ggctggctcc taaggagtac tcttatcaga tgtactcctc agaagtatac
     4261 cagagctaat agtccgaaga acggatttcc gtgaaggata gttaagttaa atcatgcgcc
     4321 tgtgggagcg catcgaattg caaccttgca aattagagcc acagaccggg aatgacgatg
     4381 atgatcggat cggatcccat gtttctgttc tcggaagcgc acgatgcgtt gatcgcttgc
     4441 tacgacttca gaatatccgg ccaaaaaaac aaaaagaggc gctatggttt ggaaatgctt
     4501 gataaatgga gacattcctc cggaattcag tcggtgatcg ctctcgccgg gagattataa
     4561 ttttagaatt gtgcgctgat ccgccgcgtc gcgcgcagcc agcacgattt cgttggtgcg
     4621 gtatatgtct ttgagcgtca gggtaggttc cttcccggcg tataccgaga gcagatagtc
     4681 gatgaatacg gaacccccgg gcggaagatc ggagatcacc ctgggttttg ctttgtccgt
     4741 catgaccgtt acgccggtgg cggccatgta ttccgccacg cccttcgttc ctgcaaggcg
     4801 cagccggtca tctccgtgtg tcggtgccga ttcggggcgg aagtaatcca tccgaagcgt
     4861 tgcggtgccg ccgttgtcga gttggaagat gctggcggtg gtgttttcca tggccttatt
     4921 gccgggcgaa ccgacgatgc cctgaaacga cgccgtagcc gtgaactccc gcccgcttgt
     4981 ccagcgcatc aggtccacca tatggatgcc gatccagggg atggtgccgc cataggtctc
     5041 ggagcgcagc atccactcgg gtctgcttcc ggccttgtag gatttctgag cggaaatcag
     5101 ggcgatttca ccgagctcac cggaatccac aatctgcttc agcgccagat aagaagggga
     5161 gtagcgcatc ggcagcagca ttccaagttg gatgccggag gcggagacag cctgctttac
     5221 gcgctcgaga tcggaagtca caatcgccag cggcttctcg gcgattacgt tgagcccgcg
     5281 tttcgcgctc gcgatgacgg ctccggcacg ctctccgttg ttattgcaga cggcaacaac
     5341 gtcgactttc tccccgtcca gcatgcgttc gtaatcggtg aactgtttgg cgccggcgag
     5401 gcgcggattt cgcgcagccc ttgccatcgc cgcgggcttg tccgatatgg cggctacctc
     5461 gacgtcgggc aaacgggaaa ggacgccggt gatttcaccg gggtgcccat caaaaccgat
     5521 aatggcgatc cggaccttgc gcggcaaacg cacttcgctt cgaggttgct gggcagacgc
     5581 gatggccgcg gcagccgcgg agaggacggc tcgccggctg accggtccga gggctgttct
     5641 tctgttcatg gtgcgattct cccacaccgc acgcgggtgg gggcgcttta ttatggtgtt
     5701 ttggcttgcg cggcttccgg caacagatcg acgcagtcga ttactccgat gaccgccatg
     5761 tcaatcggtg actggggccg ccccacgctg gtcatggcac tgaagccttc ggtaaccagc
     5821 aggacgcgat cgccaacgcc ggcatcgacg gaatcgagcg ctaccacggc atcgccgcga
     5881 tcggtgccgt ccaggttcag cggctgaacc atcagaatct ttcgtccttc atggctcgca
     5941 tgtttgcgcg tcgcaaccac ctcaccgatg accctcgcga tcagcaatgc ttcagcccgc
     6001 ttccttcgtc cgcttgatcg taagctcatc cacaatgccg acgatcgttg aatccgccgg
     6061 aacttcgtcc ggcaggaaag gaaaagtcga ctcgcggccg cggcaccagt aaaccagttc
     6121 gccggcgccg gcgcccgtgc aatccgctac gacgattctc ctgccggtgg gtttgagctc
     6181 gggagtcaat ggctggacga tgagcagcct gtgcccgtcg aggttcgcgt tctttaccgt
     6241 agcccagact ttacctacca ctttgccgag atacaaatca gactctcaat ccaggctgat
     6301 ggtgtccacg ataccgacaa tagcggtatc cacgggctta tccttgcagc cttcggccat
     6361 tcgcgcggag ctgccggaaa cgacgatcac cgtttcgcgg aaaccggcgc tcactgtgtc
     6421 cacagccact acataacccg tctcatcgcg gccctccggt gagatcggtt tcagaaccag
     6481 caacttcttg ccttcgagcc ggggatcttt tcgggtggcc accacagtgc ctacaacacg
     6541 cgccagtatc ataaatgtga gccaacggcg aaccgttccg gcggaagctg gaccgtgcgg
     6601 ttcgccggaa cgttacttcg agttcgattt tccgatcggc aggacgtctt ccaggctggg
     6661 atgaggacgc gggatgacgt gaaccgaaat cagttcgccg acgcggcgag ctgccgccgc
     6721 acccgcatcc gtagcggcac gcacggccgc gacatcgccc cgcactaggg cggtgacgta
     6781 tccggatccg atcttttccc atcccaccaa cgttactttg gcggccttca ccatcgcgtc
     6841 ggcagcctcg atcattgcga ccaggccccg cgtttcgatc attcctaaag cttcacccat
     6901 ttaacctccg cggcgcgccg taagcccgcc tgaatttcaa gccacgcccc gcattctcag
     6961 cgcctcgtcc accagcgcca ccagttcatc gcgggtcaca acaaatttgc cggcatccgc
     7021 atcctcggcc gccaccggac aaccgggctc cactccggcg cgggccttta ccccataccg
     7081 ggtacgggag tcgaacagtt tctcgacctc ctgtttcggc aagatcgtcg cgccgccgag
     7141 cagttccgcc accagagaaa tgcgggcgta gtgctccgta gtctccattt tgaagaaggc
     7201 tttatagacg tcctcgccgt agcacaccgc gccgtgattc gccatcagaa tcgcatcgta
     7261 cttgggaatg tagggcagca tcgggtccgt gagcgcgggc gtgcctggta aaccataatc
     7321 cgccagtggg acgcagccca gcccgatgat cacctccggc agcagcgcca ggttcaaagg
     7381 cttccccgcg gtggcaaatc cggtggcaac cggcgggtgc gcgtgcacga cggctttgat
     7441 atccgggcgc atgccataga tggtcaggtg catggccatc tcggaggtgc gttctttgcg
     7501 gccctccagc ttgttacctg acatgtcgca gatcaccagg tcgtcgggat gcatcatgcc
     7561 cttgcttaca cccgtgggcg tgcaaaggat cctctcctga tcgaggcgga tgctgatatt
     7621 tccgtcgtta gctgcaaccc accctttttg gtagacaagg cgacctacgt cgacgatatc
     7681 ctgtcgatat tcccgttccg tcttgggcat ccgaaccccc gattgtacca aagtgcatgc
     7741 agcgcacgtc cagtttgaaa gataccgggc aggaaagcgg atgaagcgtg agcagcttga
     7801 atttacatac atttacacct cgccaatatt cgctttacgt tgccccggta acgtgaaata
     7861 caggaggaca tgtaaatgtc gatcggtaag aaatggatga cgttcgcgac cgcgggaatt
     7921 gtctcagcgg gtcttctatt ggcccagggt ccgagcacca gccgccgtgg cggtccagga
     7981 ggcgaccctg gcgcgcggaa ggagcgaatg aagggattca tggcggagta tctcaatctg
     8041 acggaatccc agaagcagca ggctgagcag attttccagg ctgccaggga ttccgcgaag
     8101 ccgttacggg agcagatgaa aacgggtcac acacaaatga ccgatgcgat taagaccggc
     8161 aaatcggatc aggaattgca ggccattgcg gatgcacagg gtgcgctgat gggccaggtt
     8221 tcgggcatcc acgccaaagc taccgctaag ttttacgcca ttctcgatcc ggcgcaaaaa
     8281 gaaaaagccg agaagcttca caatcgcttc atgggcggta tgtttggcgc cggtcccggt
     8341 gctgcgcggg gcggtcccgg catggggcgg tctcgccagt aaagtcagta gacagagggg
     8401 cgggagcccg gaggatggcc ccgctccaat ccccttcttt ctcctcgggg agttccacgc
     8461 cgccgccggt gttataatcg aagcgattcg ctttgtcccg gcaagggagc ggtgtatctt
     8521 gccaccacga tggatctgga tcagctccat acctttctag aaatcgtcag gcttaaaagc
     8581 ttttccaaag ctgcgcaaac ctgctaccgg acccaacccg ccattagtgc gcaagtccgc
     8641 cagctcgaac aggaactgaa tgccagtctt ttcgaacgcc tgggtactcg aatcgctcta
     8701 acggccgccg gaaaaatctt cgccgaatac gccgcgcaga ttctcgatct caggcggcaa
     8761 gcccaagacg ctatcaatga gcttgaacgg gtgcctcggg gagaactggt tatcgcggcc
     8821 aacgaagcca cctgcattta cgtgctgccc gtcgtatttt ccgagtacaa gaagcaattc
     8881 ccgaacgttc aactccacgt ggaccgctcg tacgggtcgc gcgtggtgag cgcggttctc
     8941 gagaaccagg ctgatttcgg catcacccaa ttaccggtgc aagagaaaaa gcttcaggtg
     9001 gtgaagatcc attccgatga gataagagtg ttggtacctc caggacatcc gctggccaga
     9061 cgcacctccg ttgtggcgca ggacgttatc ggctgcccgt tgctgctgcc gaaatccggc
     9121 aacacccgcg cgcgcctgaa cgcctggctg gagctggtgg aggaccatat tcagatctcc
     9181 atggagttgg attcgaccga gatgatcaag cgcttcgtca tggcggggct ggggatctcg
     9241 ttcgtggccg cctcaaactg ccgtgaagag acggcttcgg gcaggctcgc caccgttgcg
     9301 ctcgcgccgg aacccatgat gcgccggctt gggctaatct acagaaagga caaagcgctc
     9361 tccaaggcgg cgctgggttt catccaggca accctggaat actcggcgtc gggggagctc
     9421 gcggtcccaa atttgaaact ggcttccacg ttatgagcgg ctcaacgttc aagcattcga
     9481 caattctcat cgcagcgggc aagcggacgg aagtgtaccg ttccctcgac caggttcccc
     9541 ccgcgctcct ccgaaagttg aaagagtgta ccggcggatc gaatgccggc actatcttaa
     9601 ttgccgacga gaggggacgc gaggagattc tacgcagctt gcgcgacggt gcaggtgcag
     9661 gcaccccggt gcgggaccgg cagcgggtgc cgcacttcga cagtctgagt tcgggacggg
     9721 catcgtggat ccgtgtcctg gaggttttta tccccactgc gctggctgtg cttgcactgt
     9781 tgattctgat ggcggaatga gttctccggt ccgccgcgtg cctttccgca accctctgcg
     9841 ataatacgta attacgtgtc aggcttggtg tgaacgttct tcgagaacct gctcc
//
LOCUS       DASTMK010000598         6404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_446259, whole genome shotgun sequence.
ACCESSION   DASTMK010000598 DASTMK010000000
VERSION     DASTMK010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446259"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFQ79_24725"
     CDS             <1..202
                     /locus_tag="VFQ79_24725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142948.1"
                     /translation="KKIWYGEMRIPIDKIDTRPPRAGNEMRINFYRIQGPPEQHRGIA
                     WQPTHSRTYHVPEAFGRLILSK"
     gene            203..1570
                     /locus_tag="VFQ79_24730"
     CDS             203..1570
                     /locus_tag="VFQ79_24730"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142949.1"
                     /translation="MPAPAALRISHVGLRGVVGRGMTASHVLDFASAFGTFLERGKPV
                     ILGRDPRASGVMIREGVVSALLACGHNVIDLGVVSTPVIQNAIRRCDAAGGISIGASH
                     NSSEWNALKFFGARGTYLSTAEAGELLDIYHLKNFSFVGWDAIGELKEQRDALDWYLD
                     ELAGAYDLAALARFSLLVDCCNGTSSLILRRLNERFGMNFTLINADIQGVSFAHEPSI
                     NRRTVALQLAPLIKPVRADAGFLFDVDSDRVAIATNEGTAISEELVLPMIADYLLPRG
                     PGKLVITNLSTTALLDEVASWHDGRVVRVPVGRQAAIDALATYRPDQIALAGEGTGAV
                     MMPRFRFVYDGIASMLGILSMMLERGKTLAEIVAGYPRYSILKGEVPLVSKQIPALLM
                     DLQRRYEGGTVNIVDGIRVDWPGRWFHVRVSQTEPVVRVICEQRGDPPVALYEELMDL
                     ARSAA"
     gene            1572..2933
                     /gene="glmM"
                     /locus_tag="VFQ79_24735"
     CDS             1572..2933
                     /gene="glmM"
                     /locus_tag="VFQ79_24735"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU0142950.1"
                     /translation="MPPREHLLKIGVSGIRGVVGEFLTPALACAFAQAFGTYVGPGRV
                     VIGRDTRASGPMLQHAVTCGLLATGCDVLDVGVLPTPTIQIFTGATRARGGIAVTASH
                     NPPEYNALKLFNAQGLFFNHYERNELVDLYHQSDFRQAVNQEIRCIFHDYDTPVTMHF
                     RRVLEHVDADRIRRKRFRVALDSVNGAGSLVSCRFLREALGCDLHAISVDPTKPFPRE
                     AEPRPDTLGELADLVRREKCEAGFGQDPDGDRLAVADENGAVLDNDDVLALAVDAALR
                     RVSGDVVVNLTTSMVVDDVASRHGRRVFRAPVGEANVVATMQSVQAAIGGEGSNGGII
                     FPAAHLCRDSYTGMAFMLDRMAETGLPMSELAGRLPCYHRKFGKVGFEHGKLGPLMQA
                     LEESFPEAQADRSDGVKLMWPDAWIHVRASNTEPLLRLAAEAKSQDRMASLYAKVVEL
                     LEN"
     gene            complement(2939..3247)
                     /locus_tag="VFQ79_24740"
     CDS             complement(2939..3247)
                     /locus_tag="VFQ79_24740"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU0142951.1"
                     /translation="MLAIVLAGVYAFVALRSPSGIPALLEKRQQVRQLQEQNATLIKE
                     IQMKRDQIKRLRDSRDEQELEIRRRLKLLRPGETSFIIPEAKPAGTPVSGVPETTKSE
                     "
     gene            3354..3680
                     /gene="cutA"
                     /locus_tag="VFQ79_24745"
     CDS             3354..3680
                     /gene="cutA"
                     /locus_tag="VFQ79_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEU0142952.1"
                     /translation="MTDKIVVLSTCGDADEAGRIARRLIEKRVAACVNIVAGVRSIYR
                     WQGSIEDSSEHLLIIKSSRPALEALQAELRAAHSYAVPEIVALPIVDGSEDYLAWLGV
                     ELGETS"
     gene            3737..5035
                     /locus_tag="VFQ79_24750"
     CDS             3737..5035
                     /locus_tag="VFQ79_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0142953.1"
                     /translation="MAAPSQPTTPAGTAVPPTRTRYWVIVFAVTLAVITYIDRVCISQ
                     AAPAISRDLGLTPVEMGYAFSAFALAYALFEIPGGWLGDYMGPRKVLMRIVIWWSFFT
                     AATGWAWNLMSLLVTRFLFGAGEAGCFPNLTKSFTTWLPQRERVRAQGIMWMSARWGG
                     AFTPYLVVLVFQYMSWRRAFELFGAIGIVWAIFFYRWYRDNPRDNKNVNAAELAMLEG
                     AESRASGHGDVPWGRFLRSRTVWLLWAQYFCLSWGWYFYITWLPTYLQQGRGIEIQES
                     AILAGLPLFFGGLGSLFCGYISTHVTRWTGSERTSRKLLSCTGFAGASALLLVSVNLA
                     DPLYAMLAMGMASFSNDLAMPPAWGACMDVGGKYAGTLSGSMNMMGNLAGAVAPVSVG
                     YILHWTHSWDLTFYISAGIYFAGTFCWMFLDPVTPLDEPA"
     gene            complement(5032..>6404)
                     /locus_tag="VFQ79_24755"
     CDS             complement(5032..>6404)
                     /locus_tag="VFQ79_24755"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0142954.1"
                     /translation="LAAAAPSCYITSWPALWTKPGPQDAEQNFPDFIRAGLDFGDFLI
                     AFFPKPVMMLTAIRDFFPIAGARNTYAEVKGVFERGDADGRAGYFEYDDEHGWSKPRR
                     EATYRWMAKWLQGRDDDGVEPEIQPEPEEALNATPTGQLATSLGGKTVRVLNRELAER
                     LFPKRKAASLRDAGDLRQLVRNRLSIVRGSGVPRVTSHGRIERQGYTIERITLETEPG
                     IDVPALVFVPSGVQGRMPAVIYANSDGKAAGAGSGGQIENIVRSGRLVLAPDARGWGE
                     SAAPKRRGGYSAEWQTANRAMLIGKTMAGSQTTDLLRSFDYLASRPDVDVAKIAVFGK
                     NKGGVIALYAAAMEPRIARVAVEGAVPSYMSVVRSETHKDLIDVVVPGVLHDFDLPDL
                     AAKAIAPRPLLVSGVRDAMGNPMGAGQAEAEFAPVKTAYSRAGHAAAFRLADGESAVD
                     LAGWLD"
BASE COUNT         1156 a         1957 c         1995 g         1296 t
ORIGIN      
        1 aaagaagatc tggtacggag agatgcgcat ccctatcgac aagatcgaca cgcggccgcc
       61 gcgcgccgga aacgaaatgc gtatcaactt ctatcgcatt caagggcctc cggaacagca
      121 ccggggcatc gcctggcaac ctacgcactc gagaacctac cacgtgccgg aggcctttgg
      181 ccggctgatc ttaagcaagt agatgccagc accggcggca cttcgcatct cgcacgtagg
      241 gctgcgtggc gtggtgggcc gcggcatgac ggcctcacac gtactcgatt ttgcctccgc
      301 gtttggtact tttctcgagc gcggcaaacc cgtgatatta ggccgcgatc cgcgcgcaag
      361 cggcgtcatg atacgggaag gcgtggtttc ggcattgctg gcgtgcgggc acaacgtgat
      421 cgacctggga gtggtctcga cacccgttat ccagaacgcc atccgccgct gtgatgccgc
      481 gggaggcatt tcgatcggcg ccagccacaa cagctccgaa tggaacgcac tcaaattttt
      541 tggggcgcgc ggaacttacc tctctaccgc cgaagccggt gagctgctcg acatttatca
      601 cctgaagaat ttcagcttcg tgggctggga cgccattgga gagctgaagg agcaacgcga
      661 cgcgctggac tggtatctgg acgaactggc cggagcgtac gacctggccg cgctggcgcg
      721 attttcgctg ctggtggatt gctgcaatgg aacctcatcg ctcattctgc ggcgtctgaa
      781 cgagcgcttc ggaatgaact tcacgttgat caacgcggac atccagggag tcagtttcgc
      841 acacgagccc agcattaacc gccgcaccgt ggccctgcaa ctggcgcctt tgattaagcc
      901 ggtacgcgcg gacgccggct tcctgttcga tgtggattcc gatcgggtgg ctatcgcgac
      961 gaacgaggga acggccatct ccgaagagct cgtactcccc atgattgccg actacctgct
     1021 gccgcgaggg cctggcaaac tggtcattac caatctctcc accaccgcgc tgctggacga
     1081 agtagcaagc tggcacgatg gccgtgtggt gcgcgtgccc gtgggccggc aggctgccat
     1141 cgatgcgctc gccacttacc ggccggacca gatcgcgctg gccggtgagg gcaccggagc
     1201 tgtcatgatg ccgcgtttcc ggttcgttta cgatggcatc gcgtccatgc tcggaatcct
     1261 ttcgatgatg ctggaacgtg gcaaaacgct ggcggagatt gtggcaggct atccgcgata
     1321 ctcgattctc aaaggggaag tcccgctggt atccaagcag attccggcgc ttctgatgga
     1381 ccttcaacgc cggtacgaag gaggcaccgt gaatatagtg gacgggattc gcgtcgattg
     1441 gcccgggcga tggttccatg tcagggtcag ccagaccgag ccggtcgttc gcgtaatctg
     1501 cgagcagcga ggcgacccac cggtcgccct gtatgaagaa ttgatggacc tggcgcgcag
     1561 cgccgcctga tatgccgcca cgcgagcatc tgctgaaaat cggggtttcc ggtatacgcg
     1621 gggtggtcgg cgagttcctg actcccgcac tggcgtgcgc tttcgcacag gcgttcggca
     1681 cctacgtagg acccggccgg gttgtcatcg ggcgcgacac acgcgccagc ggtccgatgc
     1741 ttcagcacgc ggtcacgtgc gggctgctcg ccaccggttg cgatgtgctg gatgttggcg
     1801 tgctgccgac tccaaccatt caaatcttta caggcgccac acgcgcgcgc ggcggcatcg
     1861 cggtgaccgc atcgcacaat ccacccgaat acaacgccct gaagcttttc aacgcgcagg
     1921 gccttttttt caaccattac gagcggaatg aactggtcga cctgtatcat cagagcgact
     1981 tccggcaggc cgtcaaccag gagattcgct gcattttcca cgattacgat acgcccgtta
     2041 cgatgcattt ccggcgcgtg ctggaacacg tggacgccga ccgcattcgc cggaaacgat
     2101 ttcgcgtggc gctggactcc gtaaacggcg ccggatcgct ggtgagctgc cggtttttgc
     2161 gggaggcgct cggctgcgat ttgcatgcga tttccgtaga tcccacgaaa ccgtttcccc
     2221 gcgaggccga gccgcgcccg gacacgctcg gcgaacttgc ggacctggtg cgccgtgaaa
     2281 aatgtgaggc cggtttcggg caggatccgg acggcgaccg gctggccgtc gccgatgaga
     2341 atggagccgt gctggacaac gacgacgtgt tggcgcttgc cgtggacgcg gcgctgcgcc
     2401 gggtttccgg ggacgtggta gtaaacctca cgacatcaat ggtggtggat gatgtggcct
     2461 cccggcacgg aagacgcgtc ttccgcgcgc cggtgggcga ggcgaacgtg gttgcaacga
     2521 tgcaaagcgt gcaggcggcg atcggcggag aaggctcaaa cggcggcatc atttttcccg
     2581 cggcccatct ttgccgcgac agttataccg ggatggcctt catgctggac cgcatggcgg
     2641 agaccggcct tcccatgtcg gaactggcgg gccgtttgcc gtgctaccac cgcaagtttg
     2701 gcaaggtcgg tttcgagcat ggaaaactgg ggccgttgat gcaagctctc gaagagagct
     2761 tcccggaggc ccaggcggac cggtcggacg gcgtgaagct gatgtggccc gacgcctgga
     2821 ttcatgtccg tgcatccaac accgagccgc tgctgcggct cgccgccgaa gcaaaaagtc
     2881 aggacaggat ggcgagcctc tacgcgaagg tggtggagtt gctcgaaaac tgacgggcct
     2941 actcggactt ggtggtttcg ggaaccccgg ataccggcgt gccggcaggc tttgcttccg
     3001 ggatgatgaa cgacgtctcg cctggccgca gcagtttgag gcggcggcgg atttcgagct
     3061 cctgttcgtc gcggctatcg cgcagacgct tgatctggtc gcgcttcatc tgtatttcct
     3121 tgatcagcgt cgcgttctgc tcttgcaatt gccgcacctg ttggcgcttt tccaacagcg
     3181 ccggaatgcc gctggggcta cgcaatgcca cgaacgcgta cacaccggcc agcacgatcg
     3241 cgagcaaata acccgctttc cggatcgcac ctgtgaatga accacgttcc aagagaagac
     3301 tcctacactt gtttataatc tgtttgtggt gattccgcca cataattcgc ttcatgacag
     3361 acaaaatcgt tgtgctcagc acctgcggtg atgccgatga agccggacgc atcgcgcgcc
     3421 ggctcatcga gaagcgcgtg gccgcgtgcg taaacattgt cgcgggcgtt cgcagcattt
     3481 accggtggca gggctccata gaggattctt cggaacatct gttaatcatc aaatccagcc
     3541 gtccggctct cgaggcgttg caggccgaac tccgcgccgc ccattcctac gcggtgccgg
     3601 agattgtcgc gctccctatt gtcgacggtt ccgaagatta tctggcatgg ctgggagtgg
     3661 aactgggcga gacgagctga tcgtcgcggc cctacttgca tcttttgcgg ctgccgtgat
     3721 gtgatggagc cttccaatgg cagcaccttc ccaacctact accccagccg gcacggccgt
     3781 gccccccaca cgcacccgtt attgggtgat tgtgttcgca gtcacactcg cggtgattac
     3841 ctacatcgac cgtgtgtgca tctcacaagc ggcgccggcc atcagccgcg atctcggatt
     3901 gacgccggtc gaaatgggat acgcgttctc ggcgttcgcg ctggcatacg cgttattcga
     3961 gatcccggga ggatggctgg gcgactacat gggtccgcgg aaagtcctca tgcggatcgt
     4021 catatggtgg tcgttcttca cggctgcgac tggatgggcg tggaacctga tgtcgctgct
     4081 ggtcacgcgt tttcttttcg gcgctggaga agccggctgc ttcccgaacc tcacgaagtc
     4141 tttcaccaca tggttgcccc agcgggagcg agtgcgggcg caggggatca tgtggatgag
     4201 cgccaggtgg ggcggcgcgt tcactccata cctggttgtt ctggttttcc aatatatgtc
     4261 ctggcgcagg gcctttgagc tgttcggcgc catcgggatc gtctgggcga tctttttcta
     4321 ccgctggtat cgcgataatc cgcgcgacaa caagaacgtg aatgcggcgg aactggctat
     4381 gcttgaaggg gctgaatcca gggcatccgg gcatggcgac gtgccctggg gccggttcct
     4441 cagatcgagg accgtctggc tgctttgggc gcagtatttc tgcctgtcct ggggctggta
     4501 tttctatatc acgtggctgc cgacctatct acagcaaggg cgcgggattg agattcagga
     4561 gagtgccatc ctcgcggggc tgccgctatt ttttggcggc ctgggatcgc ttttctgcgg
     4621 ctacatttcc actcacgtca cgcgatggac cggcagcgag aggacatcga gaaagctttt
     4681 gtcgtgcacc ggattcgccg gcgcttcagc tctcttgctg gtttccgtga accttgcgga
     4741 cccgctgtac gcgatgctcg cgatgggcat ggcgagtttc tcgaacgatc tcgctatgcc
     4801 gcccgcctgg ggagcctgca tggatgtcgg cggcaagtac gcgggcacgc tctccggctc
     4861 gatgaacatg atggggaatc tcgccggagc tgtggctccg gtttccgtgg gctacatcct
     4921 gcactggacc catagctggg acctgacgtt ctacatctcg gcgggaatct actttgccgg
     4981 gacgttttgc tggatgttcc tggatccggt gacgccgctc gacgagcctg cttaatcgag
     5041 ccaacccgcc agatcgaccg cgctttcacc atccgccaac cggaacgccg ccgcgtgccc
     5101 ggcgcgtgaa tacgccgtct ttaccggcgc gaactccgct tccgcctgtc cggcgcccat
     5161 cggattcccc attgcatcgc gaacgccgct gaccagaagc ggccgcggcg caatcgcctt
     5221 tgcggcaaga tcgggcaggt cgaaatcatg gagcacgccg gggacgacaa cgtcaatcag
     5281 gtctttgtgt gtttccgaac gcacgacgga catataggac ggcaccgcgc cctccaccgc
     5341 cacgcgtgcg atccggggct ccatcgccgc ggcgtacagc gcgatgaccc ctcccttgtt
     5401 ctttccaaac acggcgatct tcgccacatc cacatccgga cgggaagcaa gatagtcaaa
     5461 ggagcggaga aggtcggtgg tttgcgagcc ggccatcgtc ttgccgatca gcatggcccg
     5521 gttcgccgtt tgccattcgg cggaataacc tccgcggcgc ttgggcgccg cgctttcgcc
     5581 ccagccgcgg gcatcgggcg ccagcacgag gcggcctgag cgaacgatgt tttcgatctg
     5641 cccgccactg ccggcgccgg cggccttccc gtccgaattg gcatagatca cggcgggcat
     5701 gcggccctgg acaccggacg gaacgaacac gagcgcggga acatcgatgc ctggttctgt
     5761 ttcgagagta atccgctcga tggtgtaccc ttgcctctcg atgcggccgt gactggttac
     5821 ccgcggcacg ccggagccgc gaacaatgct caaacggttc cgcacgagct gccggagatc
     5881 cccggcatcg cgcaaagatg cggccttgcg tttcggaaat aaacgttccg ccagctcccg
     5941 gttcagcacc cgcacggttt tgccgcccag cgacgttgca agctgcccgg ttggtgtggc
     6001 gttcagagcc tcctccggtt cgggctggat ttccggctcc acaccatcgt cgtcgcgccc
     6061 ttgcagccat ttcgccatcc agcgataagt ggcttcgcgg cgcggtttcg accagccatg
     6121 ctcgtcgtcg tattcaaaat accccgccct cccatcggca tcgccacgct cgaatacgcc
     6181 tttcacctcg gcataagtat tacgggcgcc cgcaatgggg aaaaagtcgc ggatcgcagt
     6241 cagcatcatc accggtttcg gaaaaaacgc gatcaggaag tcgccgaaat cgaggccggc
     6301 gcgaatgaag tccggaaagt tctgttccgc atcctgcggc cccggcttcg tccagagagc
     6361 gggccacgag gtgatatagc aggaaggcgc cgccgcggcg agcc
//
LOCUS       DASTMK010000599         4043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_157725, whole genome shotgun sequence.
ACCESSION   DASTMK010000599 DASTMK010000000
VERSION     DASTMK010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157725"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1642)
                     /locus_tag="VFQ79_24760"
     CDS             complement(134..1642)
                     /locus_tag="VFQ79_24760"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEU0142955.1"
                     /translation="MKNARVSRRNVIQAGLATPMAAFQGQKADSGLIPRENKKPGALD
                     WQLTKVRLDKRDGFRAPDIEGYCSHQSIEAGETLRVMVSLREAGRFSLEIFRMGYYGG
                     RGARQMTTLGPIAGKPQPVPEVGPERVRECRWEPNAELKIPADWPSGVYLGRLTRIPE
                     SRNVHGWQTFIVFIVKDRRPADILFQCSDNTWQAYNRWPDDYSLYTDPRHAWAPDVSV
                     SFDRPYGKIAQIYENPQTIGSGEFLAWEYPMAYWLEMHGYDVTYCSNADMLDAKQPLR
                     AKVFLSIGHDEYWDLRQYDAAMASVKAGVTHLYLSGNSVFGVTPYSPSYDGRPNRIIS
                     RKGRYGGPAKAKRDEFFKYPFPMTGPDEALLMGARTPYPFNGGGDWICTRPDHWVFTG
                     TGMKSGDLVAGLVGWEFHGDPADIPGLEVVAEGIALSGGTRPVRWTATIYPGPKENFV
                     FNASTIFWAQGLSSPPGHMLPWSHWTRPHGPDARVQRVTQNLLRKALGKNLT"
     gene            complement(1683..3653)
                     /locus_tag="VFQ79_24765"
     CDS             complement(1683..3653)
                     /locus_tag="VFQ79_24765"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEU0142956.1"
                     /translation="MNRKLLFAGVFALSFSSLANEHPSYILNLEKTPHAKLRNVPVSA
                     VRITDGFWSARCATNAARSMPTLLALLEAHGVVDNFRRLSGKKKVARRGPLYTDSDLY
                     KWMEAAAFVLQTDRNPQLEATFDALTGDIVAAQEPSGYLNTYYHDERKAQRFEEMDRG
                     HELYCLGHLLQAAIAYHRATGKRKLLDAGIRFADYLVRDFGPDKHPLLAGHPEIELAL
                     IELYRHTDEKKYLELAGYILRGDGERLKLTRNQIQYLFSGKPFTERTIMEGHAVRAGY
                     ACAGAADYYLETGDAAYKTTLERLWNDMVSGKMYITGGIGSRAAGEAFGDPYELPNAK
                     AYTESCAAIANMIWNWRMLAASGDAKYADVLELALYNSVNSGMSLEGTLYCYRNSLEL
                     TAEAARSIRNPWYSTTCCPPNLHRTLASLPGYFYSTSADGVYVHLYDNNQLDWKLEDG
                     TPLGIAQATRYPWDGVVDIRVDPEQAKEFTIFVRIPGWSRSTQVLTGGKAVASTVKPG
                     EYLPVRRKWSAGDTLRLVFDMTPQAIVSHPRVMDNQGRAAVKRGPLVYALEQTDQPAG
                     VRVLEAGLRLSAEPSKDFTPEFRKDLLGGVVVLRHRGVTRATSFADEPLYRGIESRDG
                     KPGKPADLTLIPYYTFLNRGPAAMVVWTTWYQ"
     gene            3854..>4043
                     /locus_tag="VFQ79_24770"
     CDS             3854..>4043
                     /locus_tag="VFQ79_24770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142957.1"
                     /translation="MNTIQAISSTRTPVDLDPDQLDSLRRHLRGKLILQEDSAYEAAR
                     SVYNAMIDRHPAVIAHCVD"
BASE COUNT          788 a         1226 c         1202 g          827 t
ORIGIN      
        1 tcaggtcgtc gcctgagaaa ggcgcccaca atccatttcc ggcgaagtac aggaaccacg
       61 ccgcaaggaa aaggaccgcg gcgcagcgag tgtgcagacc gtgtgaaagc cagtcctgcg
      121 ctttgagcgg catctatgtc aggtttttgc caagagcctt acgaagtaga ttttgggtga
      181 cgcgttgcac ccgcgcgtcg ggaccgtggg gacgcgtcca gtgcgaccag ggaagcatgt
      241 gcccaggcgg cgacgacagt ccttgcgccc agaaaatggt ggaggcgttg aacacgaaat
      301 tttcctttgg tcccggataa atcgtcgcgg tccagcgcac cgggcgtgtg ccgccgctca
      361 aggcgatgcc ttcggccacc acttccagtc cgggaatatc ggcggggtcg ccgtgaaatt
      421 cccacccgac caggccggct accaggtctc cgcttttcat gcctgtacct gtgaacaccc
      481 agtgatcggg cctggtgcag atccaatctc cgccgccatt aaacggataa ggagtgcgag
      541 cgcccatcag cagcgcttca tcgggtccgg tcatcgggaa agggtatttg aagaactcgt
      601 cgcgctttgc tttggcgggg ccgccatacc ggcccttgcg gctaatgata cgattgggcc
      661 ggccgtcgta ggaaggcgag tagggcgtaa cgccgaacac cgagttgccg gataaataaa
      721 gatgggtgac gcccgccttc accgatgcca tggccgcgtc gtattgccgc agatcccagt
      781 attcgtcgtg tccgatactc aagaacacct ttgcacgcag cggctgcttc gcatccagca
      841 tgtcggcgtt ggagcagtac gttacatcgt agccgtgcat ctccagccaa taagccatcg
      901 gatactccca ggccaggaac tcgcccgagc cgatggtttg cgggttctcg taaatttgcg
      961 cgatcttgcc gtacggccgg tcgaagctta ccgaaacatc gggcgcccag gcgtgccgcg
     1021 ggtccgtata gagggagtag tcgtccggcc agcggttata ggcctgccac gtgttgtcgc
     1081 tgcactggaa cagaatatcg gcgggccttc gatctttcac gatgaatacg atgaaggtct
     1141 gccatccgtg gacgttgcgg ctttcgggaa tgcgtgtgag ccggcccagg tagacgccgc
     1201 ttggccaatc ggccgggatt ttcaattcgg cgttcggttc ccagcggcat tcgcgtaccc
     1261 gctccggacc cacttcaggt accggctgcg gtttgcccgc gataggacca agggtggtca
     1321 tctgccgcgc accgcgcccg ccatagtagc ccatgcggaa aatttcgagc gagaaccgcc
     1381 cggcttcgcg caggctcacc atgacgcgca gggtctctcc ggcttcgatg ctttgatgag
     1441 agcagtagcc ctcgatgtcc ggcgcccgga aaccgtcgcg tttgtccagc cttacctttg
     1501 tgagctgcca gtccagcgct cccggcttct tgttctcccg cggtatcaaa cccgaatcgg
     1561 ccttctgccc ctggaaagcc gccatcggtg tagcaagccc cgcctgaatc acattccggc
     1621 ggctgacacg tgcgtttttc aaaattgccc tccgtgaggt gcaatatatc aataacggcc
     1681 gctcactggt accaggtcgt ccacaccacc atcgccgcgg ggccacggtt caggaacgtg
     1741 taatagggga tcagtgtgag atcggcgggt ttgcccggct tgccgtcccg gctctcgatg
     1801 ccgcggtaca gcggctcgtc tgcaaaagag gtggcgcgag tgacaccgcg gtgccggagc
     1861 acgactacac cgcccagcag gtccttgcga aactcgggcg tgaagtcttt cgacggttcc
     1921 gccgaaagac gcagtcccgc ttcgagcact cggacacctg cgggctgatc cgtctgttcg
     1981 agcgcataca ccagcggccc gcgtttcacc gccgcgcgtc cctggttgtc catgacccgc
     2041 gggtgcgaca cgattgcctg cggcgtcata tcgaagacca ggcgcaatgt atctcccgcc
     2101 gaccatttac ggcgtaccgg aaggtattcc cccggcttca ccgtcgaagc aacggctttt
     2161 ccacctgtca gcacctgcgt ggaccggctc catccgggaa tgcgcacaaa gatcgtgaac
     2221 tccttggcct gttcgggatc cactctgata tcgacaacac catcccacgg ataccgggtc
     2281 gcctgcgcga tccccagagg cgttccgtcc tcaagtttcc aatccagttg gttgttgtcg
     2341 tacaggtgca cgtagacacc atcggcgctg gtgctgtaga agtatccagg cagcgaagcc
     2401 agggttcgat gcagattcgg cgggcaacag gttgtgctgt accagggatt ccggatgctc
     2461 cgggccgctt cggccgtcag ctcgagcgag ttgcggtaac agtacagcgt gccttcgagc
     2521 gacatgcccg agttcacgga attgtacaac gccagttcca gcacgtctgc atatttcgcg
     2581 tcgccgctcg ccgccagcat gcgccagttc catatcatgt ttgcaatggc ggcacagctt
     2641 tccgtatacg ccttggcgtt cggcagctcg taggggtcgc caaaagcttc ccctgcggca
     2701 cgcgagccga tgcctcccgt gatgtacatc ttcccggaga ccatgtcgtt ccaaagccgt
     2761 tcgagcgtgg ttttgtaggc cgcatcgcca gtctccaggt agtagtcggc cgctcccgcg
     2821 catgcgtagc ccgcgcgcac ggcatgccct tccatgatcg tgcgttcggt aaacggcttc
     2881 ccgctgaaca ggtactgtat ctggttgcgg gtcagcttca accgctcgcc atcgccgcgc
     2941 aaaatatagc ccgcgagttc gagatacttt ttctcatccg tgtggcggta taactcgata
     3001 agcgccagct cgatctcggg atgcccggca agcagcgggt gtttgtccgg accgaaatcg
     3061 cggaccaggt agtcggcgaa ccggataccg gcatcgagca gtttgcgctt ccccgtggcc
     3121 cggtgatacg cgatagcggc ctggagcaga tggcccaggc agtacagttc gtgcccgcgg
     3181 tccatctcct cgaagcgctg tgctttccgc tcgtcgtggt aataggtgtt caggtagccg
     3241 gacggctcct gtgccgcgac aatgtcgccg gtaagggcgt cgaatgtggc ctctagctgc
     3301 gggttcctgt ccgtctggag aacgaacgcc gccgcttcca tccatttgta aaggtcggag
     3361 tccgtataga gcgggccgcg acgggcaacc ttcttcttcc cgctgaggcg gcggaaatta
     3421 tccaccacac catgcgcttc gagaagcgcg agcaaggtcg gcatgcttct ggcggcattg
     3481 gtcgcacagc gggcggacca gaaaccgtcc gtaatcctga ccgcggaaac agggacgttc
     3541 cgcagtttcg cgtgcggggt cttctccagg ttcagaatgt aggagggatg ctcattggcg
     3601 agggaggaaa acgatagcgc gaaaacgccg gcaaacagaa gtttgcgatt catagaacct
     3661 ctggcataag acagccggaa acgctccaca gaataacacg ctggagatac cgtattggct
     3721 tcattctctc tctgatagat tgaaattagg ggcaactacg gtggcccact ggcactgcac
     3781 cactttgccg acgtgggagt cgactgctga gcatccgctt caagggatct ttcgaagttg
     3841 aaaggaagcg gaaatgaata cgattcaggc gataagttca accaggacac ctgttgatct
     3901 cgatccggat caactcgact cactccgaag gcacctccgg ggaaaactga ttcttcagga
     3961 agatagcgcc tacgaggccg cgcgttcggt ctataacgca atgatcgacc ggcatcctgc
     4021 ggtcatcgcc cattgtgtgg acg
//
LOCUS       DASTMK010000600        13739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_543554, whole genome shotgun sequence.
ACCESSION   DASTMK010000600 DASTMK010000000
VERSION     DASTMK010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543554"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFQ79_24775"
     CDS             complement(<1..401)
                     /locus_tag="VFQ79_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142958.1"
                     /translation="MLTRRTFLFTAAVPISAASSMTSRERVDRVLEGKEPDRRPFSYW
                     YHFGLEKYPGERHAKATLDFHRRLRTDVVKVMSDYPYPKSGGKWYELREQQNPFPEQI
                     RALELIRDGLAGRAHFVETIFNPWNQATKISS"
     gene            complement(410..1849)
                     /locus_tag="VFQ79_24780"
     CDS             complement(410..1849)
                     /locus_tag="VFQ79_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0142959.1"
                     /translation="MPMIASELKQRLIDIAGGGGVLDRPEDLLLYEYDGGVDKGRPDL
                     VVFPRTTAEVAAILEAAKESHVPVVGRGAGTGLSGGAIPRAGGVMVAFARMNRMLEID
                     LSSERAVVQPGVVNLDITLAVQPQKYFFAPDPSSQKACTIGGNVAENAGGPHTLAYGV
                     TTNHVLGLECVTPDGTVFNTSGKELDHPGYDLTGLLTGSEGTMALVTKVVVRLMRQPE
                     MVKTILAIYDSNEDAGDTVAEITARGITPVAVEMLDGVMLRMVEEATHAGYPMDAAAV
                     LLIELEGLKEAVEEQVEQVREACSASRAREFRVARSTEERDLLWKGRKNAFGAVGRVS
                     PFYYVQDGVVPRMQIARTLHYINGVSKRYGLTISNIFHAGDGNMHPIILFDARKPGEL
                     KQAQRAGEDILEYCVSVGGSITGEHGVGMEKMELMSKQFSDETLDMMRRLKALFDPEG
                     RLNPGKLLPTGKGCIEIRQPAITGAGTVY"
     gene            complement(1878..2399)
                     /locus_tag="VFQ79_24785"
     CDS             complement(1878..2399)
                     /locus_tag="VFQ79_24785"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0142960.1"
                     /translation="MRSLTADRVLQVLVGVLLIALVYVVADGIREPIVVVGDTAPKFS
                     VVTDSGLKVSRSQFGGKLLVLNFWATWCPPCIEELPSLNELHRQFSPSGLVVLGVSVD
                     RNESVYRNFLKRANIEFLTARDPGADISADYGTFRYPETYVIDRNGKVVLKIISSTNW
                     TDEKMVSYIKSLL"
     gene            2461..2877
                     /locus_tag="VFQ79_24790"
     CDS             2461..2877
                     /locus_tag="VFQ79_24790"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU0142961.1"
                     /translation="MFITRRAEFSASHVCRRADLSDAENHAMYGAAANPHGHGHNYVL
                     EVTLEGQPDPVTGMVFDLKQLKEIIHRHVVEPMDHRFLNHEVPPFDRIIPTTENVAIE
                     IWRRLAPGLNVNGVRLHSVRLYETEDLYVDYSGDER"
     gene            2874..3293
                     /locus_tag="VFQ79_24795"
     CDS             2874..3293
                     /locus_tag="VFQ79_24795"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU0142962.1"
                     /translation="MIRLTRRYRFSASHRLHAAGLSEEENRELYGKCNNPFGHGHNYV
                     LDVSVAGPVEERSGTVMRLEDLDGIVQEKIIRVLDHRNLNVEVPEFESMTPTTESLAF
                     VIGRRLRENWPAGWPRLDAVRLEETKNNRIELRAANP"
     gene            3302..3910
                     /gene="folE"
                     /locus_tag="VFQ79_24800"
     CDS             3302..3910
                     /gene="folE"
                     /locus_tag="VFQ79_24800"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU0142963.1"
                     /translation="MSERKAVVELPKPEEESIADLMKQVLARLGEDPTREGLVQTPAR
                     ADKAYRFLTSGYSMDVKRIVNGALFSVTYDEMVIVKNIEFFSLCEHHLLPFFGKVHVA
                     YLPKDKVIGLSKIPRIVDMFARRLQVQERLTREIAETIQNVVDPKGVGVICEARHFCM
                     MMRGVEKQHSGTVTSAMLGGFRDRKETRDEFLSLVSQDSLGR"
     gene            3953..4339
                     /locus_tag="VFQ79_24805"
     CDS             3953..4339
                     /locus_tag="VFQ79_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142964.1"
                     /translation="MIENFRRFEAGPDPFGRTWLVEFRWLQNAISIRHSDSIDVKFHI
                     TSGDESYVRVISLMHTDLLALSKKTDRPITDPWVTRLAALHLKKILKTGEDLEKTLIT
                     PSLADLEEHNARLVAPTPAVGDAAGR"
     gene            4388..5713
                     /locus_tag="VFQ79_24810"
     CDS             4388..5713
                     /locus_tag="VFQ79_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0142965.1"
                     /translation="MLAPNSLSGKLKELGVVGAGGAGFPTYVKANSQVEFVLANGAEC
                     EPLLHKDFELMKQFPEETVSGMRLMMGSTGARQGKFGIKEKNAEAIRAIEPHAKAGGI
                     EMVLLGDFYPSGDEYELVYTATGRLIPAAGIPLQIGCVVNNVETLYNVHFAEQGVPVT
                     QKFLSVAGAVRHPKSFWVPVGTPFRDVVRLAGGAIDSDFGIFVSGIMMGTLCFDLDEV
                     VTKTTGGLIVLPKNHYLITRKNQPQENMNRIGKSACDQCSYCTEFCPRYLLGYEVQPH
                     KVMRSLGFTSTGAEHWNQWSELCCACGLCTLYACPEDLYPKEACDQGKHDMRSAGIKF
                     VQQKPVRVHPMKEYRRVPLSMLRKRLKVEEYEAETPFENIGFTPERVRLKLSQHAGKP
                     AAPVVRQGDRVSSNDTVARVGKDELGVNIHASISGEVTSVTDRYIEIRG"
     gene            5719..6270
                     /locus_tag="VFQ79_24815"
     CDS             5719..6270
                     /locus_tag="VFQ79_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEU0142966.1"
                     /translation="MAKTSIGLIELGSIAAGFQVADAMLKAADVELVLARTICSGKYM
                     VMVRGDVAAIQAAVDAGTQAGDFSVIDTFVIPNVHEAIFPAISGITKVERLEALGIIE
                     SFSVASLIEGADAAVKAANVELLEVRLAMALGGKAFATLTGTVAAVNAAVEAGARAIA
                     RKGLLVNKVVIASPRQELLTEMI"
     gene            complement(6282..7037)
                     /locus_tag="VFQ79_24820"
     CDS             complement(6282..7037)
                     /locus_tag="VFQ79_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142967.1"
                     /translation="MVLFFVAFAIGEGFPEFTRLTAREKWMFAGLGLMLGGLILAWFR
                     EGWGGVVSIAGWSFMVIVERGMLWAWPFSIPAAIGLLHVLCWWRLRGPAPPSKPLHPR
                     TLAFLTLLGAALVAFVLLCANEMFNQPPLMTPAFRPSPEIVGSWRATLAGDVGVVFEV
                     SPDGSVSGAVGDGEVIGGKITLNRSWFGRLMNWRTDYIIRGSLSRAVEASGGTAGNRF
                     AAPLFVHGSELEGSLFLFHPGPPKPLKLKLQKH"
     gene            complement(7200..8048)
                     /locus_tag="VFQ79_24825"
     CDS             complement(7200..8048)
                     /locus_tag="VFQ79_24825"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0142968.1"
                     /translation="MTYEFGPFRYDAEQRLLFRAGEIVPLVPKAVETLHVLLERRGRV
                     VEKAELMKLVWPGTNVEDVGLARNISLLRKALEDESETNPYIETIPRRGYRFAAPVSI
                     PGPPGAPSPAPRRRVHRRWLVALLCAVALGGFVYYQFYVPSRYLPGGSRSASLAVAPF
                     ECLCPGMDGDGFSQGFNEVLAASLSKLNGVQVVSPGTVRRYQRAGISMALMGRLLGLE
                     VLVEGTIQKLGDRLRTSVRLVDVRSGKLIWAETYDEPAADPGMAQQNVARAIAREAGA
                     RLSAPR"
     gene            8315..10435
                     /locus_tag="VFQ79_24830"
     CDS             8315..10435
                     /locus_tag="VFQ79_24830"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0142969.1"
                     /translation="MNDSLKQRYILEVEPERYELFAAPAYEFAVTRRDLWRLLGAGLL
                     LTVTEPPAAGFAPVTTRIHVAENGRITLLTGKVDMGQGLRTLLTQVAAEELRTAPAAI
                     QLVTGDTGLAPDDGGTWGSLTTPETVPLVRSAAVRTRQKLIEIAAAKWSVASSELVAE
                     DGRVRDAAGRTFSYGELAGADVDDPKTTHEAPPTPPAGWRICGTDHPAVLGREIVTGA
                     LRYSSDQRVPGMLHGVLVRPSGHRATLLSYTAHAGRTLENIRVVRDGDLLGVVAADEE
                     STRRAASFLRAEWKTEPLVPFPALAAHFKKTAKPPQLQPGSRYPAIVEKGNVSSALER
                     SHTRYSSSYSLAYIAHAPLETRSAIAEWTKDAAGEKLTVRYAGQAPFLVRKELAEAFR
                     LPEDRVRVISGEIGGAYGGKQRGEVALEAARLAKAAGAPVRVHWTREDEFRSSYFRPA
                     GLVKVTSGCDARGKIVAWNFHNYNSGASSLAPPYAIADYYCGYHRSDSPLRQGSYRSL
                     AAVANTFAREMHVAELAHRHKADPLEFRLRNLDNSRMRAVLERAAERFGWGRRKSGNG
                     TGYGLACNLEKEGHLAVFVELETDGQRARVRKMTAAADFGAILNPGNLRNQIEGALIQ
                     GIGGALFERIDYDEKNVRNTSFAEYRVPRFSDTPEIEVILIDRRDIQSAGAGEAPITL
                     AAPAIGSALHAATGRWFRSMPMFG"
     gene            10457..12172
                     /locus_tag="VFQ79_24835"
     CDS             10457..12172
                     /locus_tag="VFQ79_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0142970.1"
                     /translation="MPQSQRDAEVFDVVVVGSGAGGGTVAHVLTRLGIRVALLEAGPL
                     LHPAQDFLEHKWPYEYGHRGAGEGGAAYFGRGKPFGFFTTTSGGWELEGEPYTVGEGT
                     KFRWFRSRIIGGRTNHYGRMSFRFSDYDFQPHSRDGLGTDWPITYEDIAPYYDKAEAF
                     IGIAGSREGIRSAPDGVFLPPPPPKAHEVLIKKASEKLGIPCIPNRRAVITKNLNGRP
                     ACHYCGQCGRGCITASNYSSSQVQIFPALKTGKLKIFELAMAREVLLDETGKASAVSY
                     IDKRTRRENRIRGRVIVLAASACESARLLLNSCSSRFPNGLANSSGLVGRYLMDTVGL
                     GLGGYVPALEGMPRYDTDGYGGAHLYMPWFGLGKEKELGFPRGYHIEIGGGYGMPSVG
                     LFYGMIRRHGYGRKLKESIRREYGAFVAFAGRGEMIPNHLSYCEIDPNVVDKWGIPVL
                     RFHFRWSDYELKQARHMQQTFAAIVETMGGRVTGTSARPDDNVPISVPGEIIHEVGTA
                     RMGTSAKNSVLNGYCQAHDVKNLFVVDGASFVSNPDKNPTLTINALAWRASDYMAEEM
                     RKGNL"
     gene            12172..12780
                     /locus_tag="VFQ79_24840"
     CDS             12172..12780
                     /locus_tag="VFQ79_24840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEU0142971.1"
                     /translation="MTRRHLLETIATGLTGVGAVPMSLEAAQHVHNAAEQEKRASGRY
                     TPKFLNEHEYRTVQRLAELIVPADDVSGSAVDAGAPEFIDLLCSGNAELGDIYSGGLL
                     WLDSLMRRRYTSTFVDATPQQQTGIMDELVAAARVEDASGELAPGLRFFNWVSKMTVD
                     AFYTSPLGIKDIGFQGNGVKSEYVVPAEAIEYALKRSPLGKS"
     gene            complement(12799..13245)
                     /locus_tag="VFQ79_24845"
     CDS             complement(12799..13245)
                     /locus_tag="VFQ79_24845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142972.1"
                     /translation="MTAKNILIAIGFGALLMQSPGWAQGRYRDDSRYSRNRGGYYGAD
                     VVSRVKRDVQAMARAASPRVDGEKRDLFRDTIYNLERFEYNGRRGKFDKDRLDGAIDE
                     MKRLVGSNQISRQMKSALARNIDALRNLRDGRGGSYGYYGNRDRRW"
     gene            13439..>13739
                     /locus_tag="VFQ79_24850"
     CDS             13439..>13739
                     /locus_tag="VFQ79_24850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142973.1"
                     /translation="MPERIYKLQPDRTLALRGFDDLGAAAALHSATSNSFKVSGIFRD
                     AADFAVLILYDADNFYEHPRIKYLPDFEFDGLTLEFDVRYTGLTPPDSTKYPTLDW"
BASE COUNT         2897 a         4114 c         4122 g         2606 t
ORIGIN      
        1 gaggagatct tcgtagcctg gttccacgga ttgaagatgg tttcgacgaa gtgcgcccgc
       61 cccgccagcc catcccgtat gagttcaagc gcgcggatct gttcgggaaa cgggttctgc
      121 tgctcccgca actcgtacca tttgccgccg gacttcgggt acggataatc gctcatcact
      181 ttcacgacgt cggttcgcag ccgccggtga aagtcgagag tcgctttggc gtgacgttcc
      241 ccaggatatt tctcgagccc gaagtggtac cagtaggaga aaggccgccg gtccggctct
      301 tttccctcca gcacacggtc cacccgctcg cgcgatgtca tggaagaggc ggcagaaatt
      361 ggcaccgccg ccgtgaacag gaaagttctg cgcgtcagca tagcttcctt caatagaccg
      421 tgccggcgcc ggtaatggca ggctgccgga tctcgatgca tcccttaccg gtgggaagga
      481 gcttgccggg attcagacgg ccttcgggat cgaacagggc cttcaggcgg cgcatcatgt
      541 cgagagtttc atcggagaac tgtttgctca tcagctccat tttttccatc cccacaccat
      601 gctcgccggt aatggagccg cccacggaaa cgcagtattc caggatgtct tcccccgcgc
      661 gctgcgcctg cttcaactcg ccgggttttc gggcgtcaaa cagaatgatg gggtgcatat
      721 tcccgtcacc cgcatggaag atgttgctga tcgtcaaccc ataacgcttc gaaacaccat
      781 tgatgtagtg aagcgtccgg gcgatttgca ttcgcggcac cacaccgtcc tgcacgtagt
      841 agaaaggact gacgcggccc acggcgccga aggcgttctt gcggcccttc caaagaagat
      901 cccgttcctc ggtggaacgt gcgacccgga actcgcgcgc cctcgaagcc gagcaagcct
      961 cgcggacctg ttccacctgc tcttctacag cctccttcag cccctcgagt tcgatcagga
     1021 gcacagcggc cgcatccatg ggataccctg catgggtggc ttcctctacc atccggagca
     1081 tcacgccatc cagcatttcg acggcaaccg gagtgatgcc acgagcggtg atctcggcga
     1141 cggtgtcccc cgcatcctcg ttcgaatcat agatggcgag aatcgttttt accatctcgg
     1201 gctgccgcat caggcggacc accaccttgg taaccagcgc catggtgcct tcggagcctg
     1261 ttaacaatcc ggtgagatcg tatcccggat gatccagttc ctttccgctg gtgttgaaga
     1321 cggtcccgtc cggcgtcacg cactcgagcc cgagaacgtg attagtcgtg acgccgtagg
     1381 ccagtgtatg cggtcccccg gcgttctccg cgacgttgcc gccgatggtg cacgcctttt
     1441 ggctggaggg atcaggtgca aagaaatact tctgcggctg caccgcaagc gtgatatcca
     1501 gattcaccac accgggctgc accacggcgc gctcgctcga caaatcgatt tcgagcatgc
     1561 ggttcatgcg cgcgaaagcg accatcaccc cgcccgcgcg tggaatggct ccaccgctca
     1621 gccccgtacc cgcgccgcgg cccacgaccg gaacatggga ctctttcgcg gcttcgagga
     1681 ttgccgcaac ttctgccgta gttcgtggaa agaccaccaa atcggggcgg cctttgtcaa
     1741 cgccgccgtc gtattcatag agcagaagat cttcgggccg atccagaaca ccaccgccgc
     1801 ccgcgatgtc aatcagccgc tgcttgagtt ctgaggctat catcggcata ccgtgtgggg
     1861 cgaggcatgc ccgcccttca cagcaaagac ttgatgtagc ttaccatctt ctcatcggtc
     1921 cagttggtgg aactgatgat cttcaacacc actttgccgt tccggtcaat tacataggtt
     1981 tccggatacc ggaaagtgcc gtagtcggcg ctgatatcgg cacccgggtc gcgggccgtc
     2041 aggaattcga tgttggcgcg tttcagaaaa ttgcgataga cgctctcatt acggtctaca
     2101 ctgacaccaa gaaccaccag cccggaaggc gagaactgtc tgtgcagctc gttgagagat
     2161 ggcaactctt caatgcaggg agggcaccag gtcgcccaga aattcagcac caggagcttt
     2221 ccaccaaatt gggagcggct cactttcaaa ccggagtcgg ttacaacgct gaatttcggc
     2281 gccgtgtcac cgaccacgac aatcggctcg cggattccat ccgcgaccac atacactagt
     2341 gcaataagaa ggaccccgac caggacttgc agcacgcggt ccgcagtaag actacgcatc
     2401 gtatatattt tcattttaga gtatcgccgg gcctccctga ggcatccaac cctattggag
     2461 atgtttatca cgcgacgagc ggaattctcc gcttcccatg tctgccgccg tgcggatctt
     2521 tcagatgcgg aaaaccatgc gatgtacgga gcggcagcca acccgcatgg gcacggacac
     2581 aattacgttc tggaagtgac gctcgaaggc cagcctgatc cggtcacggg aatggttttc
     2641 gatctgaaac agctcaagga gatcattcac cggcacgtgg tcgagccaat ggatcaccgg
     2701 tttctgaatc atgaggttcc tcccttcgac cgcatcatcc ccaccacgga aaatgttgcg
     2761 attgagatct ggcgccggct cgcgccgggt ttgaacgtga atggcgtgcg tctccattcc
     2821 gtccggctct atgaaaccga agatctgtat gtagattatt cgggagacga aagatgatcc
     2881 gcctgacacg ccggtaccgg ttttccgcct cgcatcggct acatgcggcg ggtctctcgg
     2941 aggaggagaa ccgcgaactc tatggcaagt gcaacaaccc cttcggccat ggtcacaact
     3001 atgtactgga tgtgagtgta gccgggccgg ttgaagaacg ctccggaacc gtgatgcgcc
     3061 tcgaggatct ggatggaatt gtgcaggaga agatcattcg ggtgctggac caccgtaacc
     3121 tgaacgtcga agtgccggag ttcgaaagca tgacgccgac cacggaatct cttgcttttg
     3181 tgatcggacg cagactgcgg gaaaactggc ccgcaggctg gcctaggctt gacgccgtcc
     3241 gcctggaaga aacgaaaaac aacagaattg aactgagagc ggcgaatccg taacgatgga
     3301 tatgagcgaa agaaaagcag ttgtggaact acccaagccg gaagaagaaa gcatagccga
     3361 cctgatgaag caggtgctgg cgcgccttgg cgaagacccc acgcgcgagg gtctggtcca
     3421 gacaccggcc cgcgccgata aagcttatcg ttttctgacg agcggctaca gcatggatgt
     3481 aaaaaggatc gtcaatggcg cgctgttctc ggtgacgtac gacgagatgg tgatcgtcaa
     3541 gaacatcgag ttcttcagcc tgtgcgagca ccatctgctg ccgtttttcg ggaaggtcca
     3601 tgtagcttat ctgccaaagg acaaggtcat cgggctgagc aaaattccgc gtatcgtgga
     3661 catgttcgcc cgccgccttc aggtccagga acgtctcacc cgcgaaatcg ccgagacgat
     3721 acagaacgtc gtcgacccca agggcgtggg agtcatttgc gaagcgcggc atttctgcat
     3781 gatgatgcgc ggcgttgaaa aacaacattc cggcaccgtg accagcgcca tgctgggcgg
     3841 ctttcgcgac cgcaaggaga cacgcgacga gttcctttca ctcgtcagcc aggactcgct
     3901 gggcaggtaa accgctgctc gcagcgccgc ctgtcggtta tcatcaaatc gaatgattga
     3961 gaacttccgc cggtttgaag ccgggccaga cccatttgga cgcacgtggc tcgtggaatt
     4021 cagatggctt cagaacgcca tctccatacg gcacagcgat tccatagacg tgaagtttca
     4081 tatcaccagc ggagacgaaa gctacgttcg cgtcatttct ctgatgcaca ccgatctgct
     4141 cgctttgtcg aagaagaccg accgtccaat cacggatcca tgggtcacga gactggccgc
     4201 gctgcatctg aaaaagatcc tgaaaaccgg cgaagacctc gaaaaaacac tgatcacgcc
     4261 ttccttggcc gacctggagg aacacaacgc acgccttgtg gcgccgacgc ccgcagtggg
     4321 ggacgcggcc ggccgctgag cctgaaccgc gcgagagccg ccccgcaccc gacgctgtta
     4381 agctggattg ttggctccaa acagtctaag cgggaagctg aaagaactcg gcgtggtcgg
     4441 tgccggtggc gccggctttc ccacctacgt gaaggcgaat tcgcaggtgg aattcgtgct
     4501 tgcgaatggc gcggagtgcg agcctttact tcacaaagac ttcgagctga tgaaacagtt
     4561 tccggaagag acggtttccg ggatgcgcct gatgatgggg tctaccggcg cgcggcaggg
     4621 caagttcggc attaaagaga aaaatgcgga ggcgattcgc gcaattgaac cccatgccaa
     4681 agccggcggc attgagatgg tgctgcttgg agatttctat ccgtccggag acgaatacga
     4741 actcgtctat accgcaacgg gacggctgat tccggcagcg ggcatcccgc tgcaaatcgg
     4801 ctgcgtggtc aacaacgtcg agaccctgta caacgtacat ttcgccgagc agggcgttcc
     4861 cgtcactcaa aaatttcttt cggtcgccgg cgccgtacgt catcccaaat cgttctgggt
     4921 ccctgtcgga acgccgttcc gtgatgtggt gcgattggcc ggcggcgcca ttgattccga
     4981 ttttggcatc tttgtcagtg gcattatgat gggcacgttg tgcttcgatc tggatgaggt
     5041 ggtgacgaaa accaccggcg gtctcatcgt gctgccgaag aaccactatc tgattacccg
     5101 caagaaccag ccccaggaaa acatgaaccg catcgggaaa tcggcgtgcg accagtgcag
     5161 ctattgcacc gagttttgcc cgcgctacct gcttggctac gaagtgcagc cgcacaaagt
     5221 gatgcgcagt ctcggcttca ccagcaccgg cgcggaacac tggaatcaat ggagcgagct
     5281 gtgctgtgcc tgtgggcttt gcactctcta tgcctgtccc gaagacctgt atccgaagga
     5341 agcctgcgac cagggaaaac acgacatgcg atcggccggt atcaaattcg tgcagcagaa
     5401 accggtgcgc gttcatccca tgaaagaata ccggcgggtg cctttatcca tgctgcgaaa
     5461 acggcttaag gtggaggaat atgaggcgga gactccattc gaaaacatcg gcttcacgcc
     5521 ggaacgggtc cgcttgaaat tgtcgcaaca cgccggcaaa ccggcggcac ccgtagtgcg
     5581 gcaaggggat cgcgtttcca gcaatgacac agtggctcgg gtaggcaaag acgaattagg
     5641 ggtgaacatc catgccagca tctccggcga ggttacctcg gtgacagaca ggtatattga
     5701 gatccgcggc tgacacaaat ggcaaagact tccatcggat tgattgagct cggcagcatc
     5761 gccgcaggct ttcaggtggc ggatgcgatg ctgaaagccg ccgacgtgga actggtgctg
     5821 gcgcgcacga tttgttcggg caaatacatg gtgatggttc gtggcgatgt cgcggcgatt
     5881 caggctgccg tggacgcggg cacgcaagca ggcgatttct ccgtcatcga cacatttgtg
     5941 atcccgaacg tgcacgaagc gatttttccg gccattagcg gcatcaccaa agtggagcgg
     6001 ctcgaagcgc tggggattat cgaatccttc tcggtcgctt cgctgattga gggcgcggac
     6061 gccgccgtga aggccgccaa cgtggaactg ctcgaagtac gcctggcgat ggctctgggc
     6121 ggaaaggcgt tcgccacgct gacaggaacc gtggccgcgg tgaacgctgc ggtggaagct
     6181 ggagcccgtg cgattgcccg caagggtctg ctggtgaaca aagtcgtgat cgcgtccccg
     6241 cgccaggaat tactgacgga gatgatctga agccagcccc gtcagtgttt ctggagcttc
     6301 aatttaagcg gtttcggcgg cccgggatgg aaaagaaaca gcgagccttc aagctctgaa
     6361 ccgtgaacga acaggggcgc tgcaaaccgg ttgcccgccg ttccaccaga ggcctcgacg
     6421 gcgcgtgaca agctgccgcg aatgatgtaa tcggtccgcc agttcatcag ccgtccgaac
     6481 cagcttcggt tcaatgtgat cttcccgccg attacctcgc cgtctcctac ggcgcccgac
     6541 accgaaccat cggggcttac ttcaaagaca accccgacgt ccccggccag cgtagcgcgc
     6601 cacgatccaa cgatttccgg tgacggacga aaggccggtg tcatcaaggg tggctgattg
     6661 aacatctcat tcgcgcaaag cagaacaaaa gccaccagcg ccgctcccag cagtgtgaga
     6721 aacgccaggg ttcgcggatg cagtggcttg gacggcggcg ccggaccccg aagcctccac
     6781 cagcacagca catgtagcag cccgatcgcc gcgggaatcg agaacggcca ggcccaaagc
     6841 attccccgtt ccacaatcac cataaaactc cagcccgcga tggaaacaac gccgccccat
     6901 ccttcccgga accatgcaag aatcagaccg ccaagcatca ggcccaagcc tgcgaacatc
     6961 cacttttccc gtgccgttag ccgggtgaat tccggaaaac cctcgccgat agcaaatgcg
     7021 acaaagaaga ggaccatcag cgtccccgtt attcgagcga tctaacggcc gatcaccgcg
     7081 gcccgccatg taacttccgt tgtagaaatc atcgcgaccg tagtattgcc ggaatccgcc
     7141 gcgaaagtag cttcggaatg tcttcggaaa accttcgcga atcttgaagt cccgttgaat
     7201 caacgcggcg cggacaggcg cgcgccggcc tcccgggcga tggcgcgtgc cacattctgc
     7261 tgggccatgc cgggatcagc ggcgggttcg tcgtaggtct cggcccagat cagcttaccg
     7321 ctgcgcacgt ccaccagccg caccgaggtc ctgagacgat cgcccaactt ctggatggtc
     7381 ccttctacca ggacctccaa tcccagaagg cgtcccatga gcgccatgga aatcccagcg
     7441 cgctgatacc ggcgaacggt accgggtgag acgacctgta cgccgttcag cttcgacagg
     7501 ctggctgcca gcacttcgtt gaatccctgg gaaaacccgt cgccgtccat gccgggacag
     7561 aggcactcga acggcgccac cgccaggctg gcggacctgc tgccgccggg cagatagcgg
     7621 gagggcacgt agaactggta gtaaacaaac ccgccgagcg caacggcgca cagaagcgcc
     7681 acgagccagc gccggtgaac gcgacggcgt ggtgctggcg agggtgcccc cggcggccct
     7741 ggtatggaaa ccggcgccgc gaaccgatat ccccggcgcg ggatggtttc gatatacgga
     7801 tttgtttcgg attcgtcttc gagggctttc cgcaacaacg agatgttccg ggccaacccc
     7861 acatcttcga cgttggtgcc gggccatacc aacttcatca gctcggcctt ttctacgacg
     7921 cgcccccgcc gctccaacag aacatgaagg gtctcgacgg ccttcgggac cagcggcacg
     7981 atttcgcccg cgcggaacag caaccgctgt tccgcgtcgt agcggaaagg cccaaactca
     8041 tatgtcatcc gacagaggta attgtagcgg agcgtctcga gggaagggtg gcgggttact
     8101 cgatgtcttt gggcgggggc gaggcgatca gggataaaaa catccccgca atgctgatca
     8161 gcgccagaac cccgaacaga tggatgacga ccatgagtcc ctcctctact cactaacgcg
     8221 taatctgttc gcgattcagg tcaaagcttt tggacgctgt gctgtctctt gcggaatggg
     8281 atacaatccg gatcagccgc cgggccgggc acccatgaac gattctttga aacagcgcta
     8341 tatcctcgag gtggaacccg aacgctacga attgttcgcc gcgcccgcct acgaattcgc
     8401 cgtcacgcgc cgggatctgt ggagattgct gggggcgggg cttctgctga ccgtgacgga
     8461 gccgccggcg gcaggtttcg cgccggtcac cacccgaatc cacgtcgccg aaaatggccg
     8521 gatcacgctg ctcacgggca aagtggacat gggccagggc ctgcgtacct tgctcacaca
     8581 ggttgccgcc gaggaattgc gcaccgcacc cgcggccatc cagttggtaa cgggcgacac
     8641 cggactggcg ccggacgatg gcggcacgtg gggcagcctg accacgcccg agaccgttcc
     8701 gctggtccgc tccgccgccg tgcgtacccg gcagaagctg atcgagattg ccgcggcaaa
     8761 atggagcgtc gcctctagtg agcttgtggc cgaagacggc cgggtccgcg atgccgcggg
     8821 ccgcaccttc agctatggcg aattagcggg cgccgacgtc gacgatccaa agaccacgca
     8881 cgaggcgccg ccaacgccgc cggccgggtg gcgtatttgc ggaacggatc atcccgcggt
     8941 gctggggcgc gagatcgtga caggcgcgct tcgctattca agcgatcagc gtgttcccgg
     9001 aatgctgcac ggcgtactgg tccggccgtc cggtcaccgc gctacgctgt tgtcctacac
     9061 ggcgcatgca ggccgcaccc tcgaaaacat ccgcgtcgtg cgcgatggcg atttgctggg
     9121 ggtagtggca gcggacgagg aatccacccg ccgcgccgca tccttccttc gggccgagtg
     9181 gaagacggag ccgctggtgc ccttccctgc cctggcggcc catttcaaga agacggctaa
     9241 gccgccccag ttgcagcccg ggtcgcgcta tccggccatc gtcgagaaag gaaacgtctc
     9301 aagcgccctg gagcgttcac acacccgtta cagttccagc tattcgctcg cctacatcgc
     9361 tcacgcgccg cttgaaacaa ggtcggccat tgccgaatgg acaaaggatg cggcgggaga
     9421 gaagcttacc gtgcgctatg ccggccaggc gccatttctg gtgagaaagg aactggccga
     9481 agcttttcgc cttccggaag accgggtgcg tgtgatatcc ggcgaaatcg gcggtgcata
     9541 cggcggcaaa cagcgcggcg aagtcgctct tgaagcggcg cggctggcca aggcggccgg
     9601 agcgcccgtc cgggtccact ggacaagaga agacgaattc cgttcgagct atttccgtcc
     9661 cgccgggctg gtgaaagtga caagcggatg cgatgcgcgc gggaagatcg tcgcgtggaa
     9721 tttccacaat tacaactccg gtgccagttc gctggcaccg ccctacgcca ttgcagacta
     9781 ctattgcggt tatcatcgca gcgattcgcc gctgcggcaa ggctcttacc ggtcactggc
     9841 agcggtcgcc aacacattcg cgcgggaaat gcatgttgcc gagctcgcgc atcggcacaa
     9901 ggccgatccg ttggagttcc gccttcgcaa cctggataac agccgtatgc gcgcggtgct
     9961 ggagcgcgcg gcggaacgtt tcggctgggg ccgtcgcaag agcggaaacg gtaccgggta
    10021 tggccttgcg tgcaacctgg agaaggaagg tcatctcgcg gtgtttgtcg aactcgaaac
    10081 tgacggccaa cgcgcccggg tgcggaagat gaccgccgcc gcggatttcg gagcaattct
    10141 gaatcccggc aaccttcgca atcagatcga gggagcgttg attcagggca tcggcggcgc
    10201 cctgttcgag cgcatcgatt atgacgaaaa gaacgtccgt aatacgtcct tcgccgaata
    10261 ccgcgttccg cgtttctcgg acacgcccga aattgaagtg atcctcatcg atcgccgcga
    10321 catccaatcc gccggcgcgg gcgaagcgcc gatcacgctg gcagcacccg ctatcggttc
    10381 ggcgttgcat gcggcgacgg ggcgatggtt ccgctcgatg ccgatgtttg ggtgaggaat
    10441 gtgatggaat ggcggtatgc cgcaatctca gagggatgct gaggtttttg acgttgtcgt
    10501 agtcggatcg ggagcaggcg gcggaaccgt ggcgcatgtg ctgacgcgct tggggatccg
    10561 cgttgcgctg ctggaagccg gtccgctgct ccatcccgcc caggatttcc tcgaacacaa
    10621 gtggccctat gagtacggcc atcgcggcgc gggagaaggc ggcgccgcct atttcggccg
    10681 cggcaagccg ttcggatttt tcaccaccac cagcggcgga tgggagttgg agggagagcc
    10741 ctacacggtt ggcgaaggaa caaagttccg gtggttccgt tcgcgaatca tcggcgggcg
    10801 gacgaaccat tacggccgta tgtcgttccg cttctcggat tatgatttcc aaccgcactc
    10861 gcgcgacggg ttgggtacgg attggcccat cacatacgaa gacattgcgc cttactacga
    10921 caaagccgag gcgttcatcg gcatcgccgg gagccgcgaa ggtattcgca gcgcacccga
    10981 cggcgtattt cttccgccac ccccgccgaa agcgcacgaa gttcttatca agaaggcgag
    11041 tgaaaagctc ggtatccctt gcatccccaa ccgccgcgcc gtcatcacga agaacctgaa
    11101 cggccggccg gcgtgccact attgtggcca gtgcgggcgc ggatgcatca ccgcgtccaa
    11161 ctattcgtcg agccaggtgc agatttttcc ggctctgaag actggcaagc tgaaaatttt
    11221 cgagcttgcc atggcacggg aggtgctact cgatgaaacg ggaaaagcct ccgccgtatc
    11281 ctacatcgac aaacgaaccc gccgcgagaa ccgcattcga ggccgcgtca ttgttctggc
    11341 ggcgagtgcg tgcgagtcgg cacggctgct tctaaactcc tgttccagcc ggttcccgaa
    11401 cggtctggca aacagctccg ggctggtggg ccgctatttg atggatacgg ttgggctcgg
    11461 gctgggcgga tatgtcccgg cgctcgaagg catgccgcgc tacgacacgg acggctatgg
    11521 cggggcacac ctctacatgc cctggttcgg gcttggcaaa gaaaaggagc tggggtttcc
    11581 ccgcgggtat cacatcgaaa tcggcggcgg atatggaatg ccctccgtcg gcttgtttta
    11641 tggaatgatt cgccggcatg ggtacggcag gaagcttaag gaaagcattc gccgcgaata
    11701 cggcgctttc gtagcctttg ccggccgcgg agaaatgatc ccgaaccatc tcagctattg
    11761 cgagatcgac cccaatgtgg tggacaaatg gggaattccc gtgctgcgat tccatttccg
    11821 ctggagcgat tatgagttga agcaggcgcg gcatatgcaa cagacctttg ccgcgatcgt
    11881 tgaaaccatg ggagggcgcg tgacgggaac atccgcccgg ccggacgaca acgtaccgat
    11941 atcggtgccc ggagagatta ttcacgaagt aggaaccgcg cgcatgggaa cgagcgcgaa
    12001 gaactccgtg ctgaacgggt attgccaggc acacgatgtg aaaaacctgt tcgtggtgga
    12061 tggcgcatcg ttcgtgagca atcccgacaa aaacccaaca ctgaccatca acgccctcgc
    12121 ctggcgcgct tcggactaca tggcggaaga gatgagaaag gggaatctct gatgacgcgg
    12181 cgccatctgc tggaaaccat cgccacaggg ctgaccgggg tgggcgcggt tccgatgagc
    12241 ctggaagcgg ctcagcacgt gcacaatgcc gcggaacagg aaaagcgagc ctctggccgt
    12301 tacacgccca agtttttgaa cgagcacgaa tacaggaccg tgcagcggct cgcggaactg
    12361 atcgtgccgg ccgacgatgt ctcgggcagc gcggtggatg ccggcgcgcc ggaatttatc
    12421 gatttgctat gcagcgggaa cgcggaactc ggagacatct attcaggcgg tttgttgtgg
    12481 ctggacagtc tcatgcgccg gcgttatacc agcacattcg tggatgccac gccgcagcag
    12541 cagaccggga tcatggatga actggtcgcc gccgcacgcg tggaagacgc atccggtgaa
    12601 ctggctcccg gtttgcgctt cttcaactgg gtatcaaaga tgaccgtcga cgccttctac
    12661 acgagtcccc tcgggatcaa agacatcggc tttcaaggaa acggggtgaa atcggagtac
    12721 gtagtgcccg ccgaagcgat cgaatacgca ctgaagcgaa gtccgttggg gaagagctga
    12781 gcctgagcgc gcggtcactc accagcgccg gtcgcggttc ccatagtagc cgtacgagcc
    12841 gccccggccg tcacgcagat tgcgcagcgc gtcgatattg cgcgccagag ccgatttcat
    12901 ctggcggctg atctgattcg aacccaccag ccgcttcatc tcgtcgatcg caccatcgag
    12961 gcggtcctta tcgaacttgc cgcgccggcc gttatattcg aagcgctcca ggttgtagat
    13021 ggtgtcgcgg aacaggtcgc gcttctcacc atcgacacgg ggggaggcgg cgcgcgccat
    13081 tgcctgcacg tcccgcttca cgcggcttac gacatccgct ccgtagtatc cgccgcgatt
    13141 ccggctgtaa cgggaatcgt cgcggtatct gccctgagcc cagcccggcg attgcatcag
    13201 gagggcgcca aacccaattg cgatcaggat gttttttgca gtcatggcgg ctcaccaatc
    13261 aaactgacgt gcggcgcgcg atccggttac cactcaaaaa acacgttagt ccggagcaca
    13321 cgttcacaag tccgcgggat ggagctaaca ggctgaaatg ctttcagtta gcaaatggat
    13381 aagggaccgg aacacccgaa aatgccgccc gcatcgtgta acatcgagac ggccagttat
    13441 gcctgagcgg atctacaaat tgcagcccga ccgaacgctg gcactacgcg ggttcgacga
    13501 ccttggcgcg gccgccgcgc tccattcggc gacctcgaac agcttcaagg ttagcggcat
    13561 ctttcgcgac gcggcggatt ttgccgtgct gattctgtac gatgccgaca acttctacga
    13621 acacccgcgt atcaaatatc tgcccgactt cgagttcgac gggctcaccc tcgaattcga
    13681 tgtgcgctat acgggactga cgccgcccga ctccacgaaa tacccgaccc tcgattggg
//
LOCUS       DASTMK010000601         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_606944, whole genome shotgun sequence.
ACCESSION   DASTMK010000601 DASTMK010000000
VERSION     DASTMK010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606944"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VFQ79_24855"
     CDS             <1..1091
                     /locus_tag="VFQ79_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0142974.1"
                     /translation="KTFNPRKFNPADWAVLAKLAGIRYVVFTAKHHNGFAMFHTKTTG
                     FGIGATPFRRDVTREILDAFRNQGIAPGLYYSPDDFFWLYRHGKIIDRNRPELAPSSD
                     PEFLKYDQAQVRELLTNYGDIDVIFFDGSPEGLRELAWKLQPDIVVTRGAMETPEQFI
                     PGVPLDGPWEACLTMGTQWQYKPTNEKYKSGGELIELLIETRAKGGNLLLNVGPKPDG
                     ELPIEQEERLREIALWMFVNGESIHGVRPWVVTNEGDIWFTKKRGEDTVYAIVKQKKP
                     WKFGEWRDIALASVRATDKTQVTVLGQNDKVLEYKSDVVPKTAWKQEPDRLTIRAMRA
                     QRLYNDYRWPNPVVLKLTNVLVSRAAGK"
     gene            1140..2105
                     /locus_tag="VFQ79_24860"
     CDS             1140..2105
                     /locus_tag="VFQ79_24860"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0142975.1"
                     /translation="MSISRREFLETTALSGIAMTAAGAEVDKKTGMPMRVLGKTGARV
                     SILAFGSGSRWLAYKEEDKALEAMNKALDLGVNYIDTAYGYGNGLSEERVGKLLKARG
                     RKGLWIATKVDARPGDDAKRIIEGSLKRLQLDQVDLLHVHSLTGEEDLKAVEAKGNVL
                     DVVRQMRDQKVCRFIGVTSHTNPTVLATALERHDFDCTQMALNAAQAAQVSARQAQSI
                     KGHFETTALPIAKRKNMGVTAMKIFAQERLSGKAPVEQLIRYSMSLPVAAAVIGMPKL
                     EHIEQNIQVAKSFKPMSKEEMQDLSGRLSQYQLALDRFFADHVDA"
     gene            2167..3060
                     /locus_tag="VFQ79_24865"
     CDS             2167..3060
                     /locus_tag="VFQ79_24865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU0142976.1"
                     /translation="MKKLAALLLLLVVGGLTIPLVWPKRADLLVQRIKGNIQIAEGCS
                     VLTPGIFGDEERFERTGKEVREIQRDGKLALYDTAIGSLWYRADSWTLPALVQENEID
                     PYRFKEIVKPGDVVLDVGANVGTDAKSALAVGASLVVAIEPEPLTLECLRRNLEAEIR
                     DSRVVVVPKGAWNREDTLLLHVDEANAGGSSFLWQKGGPSIAVPLATIDSMVAELQLP
                     KVDVIKMDIEGSEKNALLGAAETIRRHHPRLAIALEHNVNDVTALPAVARELWPDYQL
                     ELTPCTKTLGLVHPRTALLTP"
     gene            complement(3123..>3435)
                     /locus_tag="VFQ79_24870"
     CDS             complement(3123..>3435)
                     /locus_tag="VFQ79_24870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU0142977.1"
                     /translation="LSFGVVMNEAMQDSVKITVIATGFEREGLPTIARRAKELAASDP
                     FGTAAAAPAPEPMFVESVSEEATVAAQSEEQQEFFTPEPEPADELDVPAFLRKERRMV
                     Q"
BASE COUNT          766 a          980 c         1056 g          633 t
ORIGIN      
        1 cgaaaacttt caacccgcgc aaattcaacc ccgcggactg ggccgtgctg gcgaaactgg
       61 cgggaatccg atacgttgtc ttcaccgcaa aacaccataa cgggttcgca atgtttcata
      121 cgaagaccac cggattcggc atcggcgcta cccccttccg gcgggatgtc acccgggaga
      181 ttcttgatgc ctttcgcaac cagggcatcg cgccggggct ctactattcg ccggacgact
      241 tcttctggct ctaccggcat ggaaagatca tcgaccgcaa ccggccggag ctggcgcctt
      301 cctccgatcc ggagtttctg aaatacgatc aggcccaggt ccgcgagctg cttacgaatt
      361 atggcgatat tgacgtgatt tttttcgatg ggtcgcctga gggtttgcgg gagcttgcct
      421 ggaaactgca gccggacatc gtcgtcaccc gcggagcgat ggagaccccg gaacaattca
      481 ttccaggggt tccgctcgat ggcccgtggg aggcatgcct gaccatgggc acgcagtggc
      541 agtacaaacc caccaacgag aagtacaaat ccggcggaga actgatcgaa ttactcatcg
      601 agacacgcgc caagggtggc aatctgcttc ttaacgtggg accgaagccc gatggcgaat
      661 tgcccatcga acaggaagag cgtttgcggg aaatcgcgct ctggatgttc gttaacgggg
      721 aatccattca tggcgtgcgt ccgtgggtgg ttacaaacga gggcgatatc tggttcacga
      781 agaagagagg cgaagacacc gtctacgcga tcgttaaaca gaaaaagccc tggaagtttg
      841 gtgagtggag agacattgcg ctggcctccg tacgcgctac ggacaaaacg caggtaaccg
      901 ttctcgggca aaacgacaag gtgctcgaat ataaatccga tgtcgtaccc aaaacggcgt
      961 ggaaacagga gccggaccgg ttaaccatcc gcgccatgcg cgctcaacgc ctgtacaacg
     1021 actaccggtg gccgaatccg gtggtgttga agctgacaaa cgtactggtt tcgagggccg
     1081 caggcaaata gcccgcgccg ctgcggtatc atgtggtcaa gatttcgaaa ggacggctta
     1141 tgagcatctc cagacgggaa ttcctcgaga cgacagctct ctccggcatc gcgatgaccg
     1201 cggcgggagc cgaggtggat aagaagaccg gcatgcccat gcgcgtgctc ggcaagaccg
     1261 gcgcccgggt ttctattctt gcgttcggct ccgggagccg ctggctggcc tacaaggaag
     1321 aggacaaggc gctcgaagca atgaacaagg cccttgacct cggcgtcaac tacatcgaca
     1381 ccgcttacgg atacgggaat ggcttgagtg aagagcgtgt cggcaagctg ctcaaggcgc
     1441 gtgggcgtaa aggattgtgg attgcgacca aggtggacgc ccggcctgga gatgacgcca
     1501 agaggatcat cgaaggcagc ctcaaacggc tgcaactcga ccaggtcgac ctgcttcatg
     1561 tgcacagcct gacgggcgaa gaggacctga aagcggtgga agcaaagggc aacgttttgg
     1621 acgtggtgcg ccagatgcgc gaccagaaag tctgccgctt cattggcgtc accagtcaca
     1681 ccaaccccac tgttctggcg actgcgttgg aacggcacga tttcgattgc acgcagatgg
     1741 ccctgaatgc ggcgcaggcc gcccaggtct ccgcacgcca ggctcaaagc atcaaggggc
     1801 acttcgaaac cacggcgctc cccatcgcca agcgcaagaa catgggagtg accgcgatga
     1861 agatcttcgc ccaggagagg ctgagcggca aggctccggt ggagcaactc atccggtatt
     1921 cgatgtcact gcctgtcgcg gcggccgtca tcggaatgcc gaagctggag cacatcgagc
     1981 agaatattca ggtggcgaaa tcgttcaagc cgatgtccaa ggaagagatg caggaccttt
     2041 ccggacgttt gagccagtat caactggcgc tcgaccggtt cttcgccgat cacgtggacg
     2101 cttgacaccg cgcgccatcc ccggcgcatc accaaagggg aagaagctct accagaggag
     2161 tatgatgtga aaaaactggc agcgctcctg ttgttattgg ttgtcggagg attgacgata
     2221 cctcttgtct ggccgaagcg ggcagacctg cttgtacaaa ggatcaaagg gaacattcag
     2281 atcgcggagg gttgcagcgt tctcaccccg ggcatcttcg gcgacgagga aaggttcgag
     2341 cgaacaggca aagaagtccg cgagatccag cgcgacggca aattggcact gtacgatacg
     2401 gcgatcggtt ccctatggta tcgcgccgat agctggacat tgcccgcgct cgtgcaagaa
     2461 aacgaaatag atccttaccg ttttaaggaa atcgtgaaac ccggcgatgt cgtactggac
     2521 gttggcgcca atgtcgggac cgatgccaag tccgccctcg ctgtgggggc gtctctggta
     2581 gtggccatcg aacccgagcc cctcactctg gaatgtctgc ggcgaaatct ggaagcggag
     2641 atccgcgata gccgggttgt cgttgtgccg aagggcgcat ggaaccggga ggacactctg
     2701 ctgctgcacg tggacgaggc gaacgcaggc gggtccagct tcctctggca gaaaggcggc
     2761 ccgtccatcg ccgtacccct ggcgaccatc gatagcatgg tggccgaact ccaactcccg
     2821 aaggtcgacg tgatcaagat ggatatcgag ggctcggaaa aaaatgcgct actcggggcg
     2881 gcggagacca tccggcgtca tcatccgcgg ctcgcgattg ccctcgaaca caatgtgaac
     2941 gatgtaacgg cgctgcccgc ggtagcacgc gagttatggc cggattacca actggagttg
     3001 acgccgtgca ccaagacact gggcctggta catccacgca cggcgttgct gacgccgtaa
     3061 gcggctcggg aaccgagccg cgcgcgcggc tccgttccga gtaagccgca tcaatccgcg
     3121 gcctactgca ccatccgccg ttctttgcgc aggaacgccg ggacatccaa ctcatccgcc
     3181 ggttccggtt cgggtgtgaa gaattcctgc tgctcttcgc tctgtgccgc aaccgtcgcc
     3241 tcttcggaga cagattcgac aaacatcggc tcgggggcgg gcgccgcggc cgctgtgccg
     3301 aacgggtcgg aggcggccag ttctttcgcc cggcgggcta tcgtaggtaa gccttctctt
     3361 tcgaaaccag tggcgatgac ggtgatcttg acggaatcct gcattgcctc attcatgacg
     3421 acaccgaagc tgagg
//
LOCUS       DASTMK010000602         4995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_477887, whole genome shotgun sequence.
ACCESSION   DASTMK010000602 DASTMK010000000
VERSION     DASTMK010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477887"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1290
                     /locus_tag="VFQ79_24875"
     CDS             55..1290
                     /locus_tag="VFQ79_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142978.1"
                     /translation="MMERLLQFSLNYTEPQNFGQCCAFLLVTGPEWNRTYTSAYDSVV
                     SFPLIARIFPVLLFTALTVSGAAAQTAGRTRNVIFVMTDGLRWQEVFQGADSALMNKE
                     SGVPDVEALKREYWRDTPAARRETLLPFLWSVVARNGQIYGNRDAGSDAYLTNGLNFS
                     YPGYSETLCGFADPRVDSNDPTPNPNVTVLEWLHNKPAYRGKVAAFGAWNVISAIVNA
                     GRAGFPVNSGYDPYTVPPSSPRMELLNRLKEETRVWNVEPFDAFTFHTALEHLKQHKP
                     RVLFLSLGETDEWSHDGRYALYLKAAARVDGYLKELWETVQSMPEYRGTTSLVFAVDH
                     GRGRAPVEWRSHGQKIPESKYVWMAYMGPGIPALGERKNAPAVTQSQIAATVAALLGE
                     DYSGDVQKAGKPIADVLPR"
     gene            complement(1307..2926)
                     /locus_tag="VFQ79_24880"
     CDS             complement(1307..2926)
                     /locus_tag="VFQ79_24880"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0142979.1"
                     /translation="MLRRELLCGLTAGVAGRLHGAGERLIECRIVDKESGRPTPARIR
                     LMDSRGRQVVPHGHPPSLTEEAHEGDVRFQSRRFSYVDGSFQVNPSDLPLKYQVIKGY
                     EYEMAEGEIEAGPSATIPLARWSRISRGGWFSGDIHIHHISPKTCRLEMDAEDLHVAN
                     ILTSDFTADQDQFEGQVNANSGPERLIYVSQEFRQHQLGHLCVLNLKRLIEPVMVARP
                     EHYPLLATACDEARAQGGYVSWAHFPSWPGVEGPLDVALEKLDGLEILSVLEPRDFPV
                     FMRQVVPEVEANDGLRLWYRYLNCGYRLTATAGTDKMTNYVTVGANRVFAHADHGEFT
                     YQSWIDALKRGRTFITNSPILSFTVNGREPGETIKSARPQVLRIEAKAESQLPYHRLE
                     IVANGEVIADATPSGARHSAAIRVEHTPSSSCWLAARAVEGIRPYRQRGVPFPAIHVK
                     EGTLHGNYFGTRRPETVFAHSSPVYVVVGGAPVRSYDDAGYYVRYMEKCIDWLDKEGK
                     FLRPSDKRATLDVFREGRARWLKRQEEAQHR"
     gene            3034..4170
                     /locus_tag="VFQ79_24885"
     CDS             3034..4170
                     /locus_tag="VFQ79_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142980.1"
                     /translation="MMEPAYPAARAAAASVHAHFARHAAAPAAGEGKPASLPDRDAIE
                     AIIDTAFWASLRHEEGFSPRISLAFVTPAEVERALHFERALPLAARPLTRLAPAVEGP
                     GIHLGVWREREDLRVWGTTRRLPPFCFVLEVVAPGLLVVKQSRGEETGKFVNVAVLEG
                     EQIKIIDQHAATLPDCPDLLTSLLGLEARFAPDGASNVLIQIAVSMRAHGRGGLLLVV
                     PAGSDAWRKSIIHPVTYSIVPPFSVLADLMQEDPGERPKRRWQDALRRAVDGIGGVTA
                     VDGATVITDQYELLAFGAKIVRRPGSNQVDRVLMTEPIIGAAPEILEPTQLGGTRHIS
                     AAQFEHDQRDAVALVASQDGRFTVFAWSPCENMVHAHRIESLLL"
     gene            4350..4826
                     /locus_tag="VFQ79_24890"
     CDS             4350..4826
                     /locus_tag="VFQ79_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0142981.1"
                     /translation="MKPKNTICLWFDKDAQEAARFYAATFPDSEVTAVFKAPSDYPGG
                     KKGDVLTVEFTVLGIPCLGLNGGPAFRHSEAFSFQIATDNQKETDRYWNAIVGNGGQE
                     SQCGWCKDRWGLSWQITPRTLTEALAAGGGEAKGAFEAMLTMKKIDVAAIEAARRG"
BASE COUNT          978 a         1552 c         1514 g          951 t
ORIGIN      
        1 gctctgcacg agttacgagg gtggtgatgg cgcgccaggg gacttgcgga ccacatgatg
       61 gagcggctgc tccagttctc cctcaactac acggaaccgc agaactttgg acagtgctgc
      121 gcgtttctcc tcgtcacagg cccggagtgg aaccgcacct acacatcggc ttatgattct
      181 gtagtgtcct ttcctttgat cgctcgcatt ttcccggtac tactctttac ggccctgacc
      241 gtttccggcg ccgccgcgca aaccgccggc aggactcgaa acgtcatctt cgtcatgacc
      301 gacgggcttc gctggcagga agtgttccag ggagccgatt ccgcactcat gaacaaagag
      361 agcggcgtcc cggatgtgga agcgctcaag cgtgagtact ggcgcgatac acctgccgca
      421 cggcgggaaa cgcttctgcc gttcctatgg agcgttgttg cgcgcaacgg gcagatttac
      481 ggcaatcggg acgccggctc cgatgcgtat ttgaccaatg gcctgaactt ttcgtatccg
      541 ggctatagcg agacgctctg cggtttcgcc gaccctcgcg tcgacagcaa cgatcctact
      601 ccgaatccca acgtgaccgt actcgaatgg cttcacaaca agcctgccta ccgcggcaaa
      661 gtggccgcat tcggagcctg gaatgtcatc tccgccatcg tcaacgccgg gcgcgccggc
      721 ttccccgtca attcgggcta cgacccgtat accgttccac cctcctcgcc acgcatggag
      781 ttgctgaacc gcttaaaaga agaaacccgt gtttggaacg tggagccgtt cgacgccttt
      841 acgtttcaca cagcgctcga gcatctgaaa caacacaagc cgcgcgttct gtttctgtca
      901 ttgggcgaaa cagacgagtg gtcccatgac ggccgatacg ccctctacct gaaggccgcc
      961 gcgcgagtgg acgggtacct gaaggagttg tgggagaccg tgcaatcgat gccggagtac
     1021 cgcggcacaa ccagcctggt tttcgcggtg gaccacgggc gtgggcgggc gccggtcgag
     1081 tggaggagcc acgggcagaa gatccccgaa tccaagtatg tctggatggc gtacatgggg
     1141 cccggtatcc ccgcgctggg cgagcggaaa aatgctcctg ccgtcacaca aagccagatc
     1201 gcggcaaccg tcgcggccct gttgggggaa gactatagcg gagatgtaca gaaggccggc
     1261 aaacctatag cagacgtact gccgcgatga ggcctgcccc accgcctcag cggtgttgcg
     1321 cttcctcctg ccgcttcagc catcgcgccc ggccttcccg gaacacatcg agcgtggctc
     1381 gcttgtccga aggcctcagg aactttccct ctttatccag ccagtcgata catttttcca
     1441 tgtaccggac gtaatatccc gcatcgtcgt agctgcggac cggtgctccg cccaccacta
     1501 cgtacactgg cgaggaatgc gcgaacaccg tctccggacg gcgcgttcca aagtagttgc
     1561 catgcagcgt cccttccttc acatgaatgg cgggaaaagg aacaccgcgc tggcgatagg
     1621 gcctgatgcc ttccactgcg cgcgccgcca gccagcaact tgaactgggg gtgtgttcca
     1681 cccggatcgc cgccgagtgc cgggcgccgg aaggggtggc gtcggcgatc acctcgccgt
     1741 tggcgacaat ctccaaccgg tggtacggca actgtgactc ggctttggcc tcaatccgga
     1801 gaacctgggg ccgggcggac ttgatcgtct cacccggttc gcgcccgttc accgtaaacg
     1861 acagaatcgg actgttcgtg atgaacgtgc ggcctcgctt cagcgcgtca atccaactct
     1921 ggtaagtgaa ctcgccgtgg tccgcgtgcg caaacacgcg gttcgctccc acggtgacgt
     1981 agttggtcat cttgtccgtg ccggcggttg ccgtcaggcg atagccacaa ttcagatagc
     2041 ggtaccagag ccgtagtccg tcgttagcct ccacttcagg caccacctgc cgcatgaaga
     2101 ccggaaaatc gcgcggctcg agaacggaca ggatttcgag accatccaat ttttccagtg
     2161 cgacatcgag cggtccctcc acgccgggcc aactcgggaa gtgcgcccag gaaacatagc
     2221 cgccttgcgc gcgcgcttca tcgcaagccg tggcgagcag cggataatgc tcggggcgtg
     2281 ccaccatcac gggctcgatc aggcgtttga ggttcaacac acaaagatgt cccagttgat
     2341 gctgccggaa ctcctgcgag acataaatca gccgctccgg ccccgagttc gcgtttacct
     2401 gtccttcgaa ctggtcctgg tccgccgtaa aatccgacgt gagaatattc gctacatgca
     2461 ggtcttccgc gtccatctcc aggcggcacg ttttggggga aatgtggtgg atgtggatgt
     2521 cgccgctgaa ccatccgccg cgggagatgc gggaccatcg cgccagtgga atggtggcgg
     2581 aagggccggc ttcgatctct ccttccgcca tctcgtactc ataccctttg atcacctgat
     2641 atttcagcgg aagatcggac gggttcacct ggaaagaacc gtcgacataa gagaagcgcc
     2701 gcgattgaaa tcgtacatcg ccttcgtgcg cctcttccgt aagactcggc ggatggccgt
     2761 gaggaactac ctgccggccg cgtgaatcca tcagccggat gcgggcagga gtaggccgtc
     2821 ccgactcctt atccacgatg cggcattcta tcagccgctc cccggcgccg tggagacgcc
     2881 cagccactcc cgcggtcaaa ccgcacaaca gttcccgtcg cagcatggtg aacccagtct
     2941 acgtcattgt gcggcggcac ctgtccatcg caaactgaaa taaatgctgg aacccaaccg
     3001 ctacaggcaa ggaaccataa tgacggtggc gcaatgatgg aacccgccta ccctgcggca
     3061 cgagcggcgg cggccagtgt gcatgcccac ttcgcccgcc atgcggctgc acccgcggcc
     3121 ggggaaggga aaccggcatc gcttcccgac agggacgcga ttgaagcgat catcgataca
     3181 gccttctggg ccagcctgcg ccacgaagaa ggcttctccc cgcggatctc gcttgccttc
     3241 gttacgcccg cggaagtaga gcgcgcgttg cattttgaac gtgcgctacc gctcgccgcc
     3301 cggcctttga caaggctggc gcccgccgtg gaaggcccgg gtatccatct gggcgtgtgg
     3361 cgggagcggg aagacctgcg tgtctggggt accactcgca ggcttccgcc gttctgcttc
     3421 gtgctcgagg ttgtcgcgcc cggcctgctg gttgtcaagc aaagccgcgg tgaggagacc
     3481 ggcaagtttg tcaacgtggc ggtgcttgaa ggcgagcaga tcaaaattat cgaccagcat
     3541 gccgcaacgc ttccggactg tccggatctg ctcacatctc tgctcggatt ggaagcgcgg
     3601 ttcgctcctg acggagccag caacgtgctg atccagatcg ctgtctcgat gcgggcccac
     3661 gggcgcggcg gtcttctgct cgtcgtaccc gcgggcagcg acgcctggcg gaaatcgatc
     3721 attcatcccg tgacgtactc gattgtgcct cctttttcgg tgcttgccga cctgatgcag
     3781 gaagacccgg gcgagcggcc gaaacggcgg tggcaggacg cgctacgccg ggccgtggac
     3841 ggaattggag gagtgacggc cgtcgatggg gcgaccgtga taaccgatca gtacgaactt
     3901 ctggctttcg gcgcgaagat tgtccggcgt cccggctcaa accaggtgga ccgcgtcctc
     3961 atgaccgagc cgattatagg tgcagccccc gaaatcctgg agccgacgca actcggtggc
     4021 acccggcata tctccgccgc gcaatttgaa catgaccagc gggatgccgt ggcgctggtc
     4081 gcatcccagg acggccggtt cacggtcttc gcatggtcgc cgtgcgagaa catggttcac
     4141 gcgcaccgca tagagagcct gctgctgtga acgcctccgg ccctgacgcg gctggctaca
     4201 agcgctacga tatgattcag aaaggacgag tggcgaccac tttgaaacgc tggctgcaac
     4261 cggtcagcaa atgaactggt gggtcaccga tagggaccga tgctataccg tcggcgttcg
     4321 ccctgatcga gcacaaggag tacacgacca tgaagccaaa gaatacgatc tgcctctggt
     4381 tcgacaagga cgcgcaggag gcggcgcgct tctatgccgc caccttcccg gatagtgagg
     4441 taaccgccgt tttcaaggcg cccagtgact atccgggcgg caagaagggc gatgtgctga
     4501 cggtggagtt caccgttctg ggcattccct gcctcggtct caacggcggt ccggcgttca
     4561 ggcacagcga ggcgttttcc ttccagattg cgacggacaa tcagaaagag acggaccgct
     4621 actggaacgc gatcgtcggg aatgggggcc aggaaagcca gtgcggttgg tgcaaggatc
     4681 gctggggcct ctcctggcag atcaccccac gcacgctgac ggaggcgctt gcggctggcg
     4741 gcggtgaggc aaagggtgcc ttcgaggcga tgctgacgat gaagaagatc gacgtcgccg
     4801 ccatcgaggc ggcgcggcga ggctgacgcg aacacgaccg cactttctca ctaacccaag
     4861 ttcgtcacgg gaccgtcgat tttaacgatt ttgaggcttt ggacgggagc aagtcctgcg
     4921 tttgcaatca ccacttctga aaatcaagat gtagtgtaga cctaccctct tgtgtcgtcc
     4981 gcaaccccat gacac
//
LOCUS       DASTMK010000603         8743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_93807, whole genome shotgun sequence.
ACCESSION   DASTMK010000603 DASTMK010000000
VERSION     DASTMK010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93807"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2352
                     /locus_tag="VFQ79_24895"
     CDS             <1..2352
                     /locus_tag="VFQ79_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEU0142982.1"
                     /translation="GVGTTGYAKDILKDVIGADAAVVETVAHTQAGLHFYDNVDVICD
                     VGGQDIKIIILKNGRVKDFKLNTQCSAGNGYFLQSTSQGFGIPVEQFADTAFGANGFP
                     MFGYGCAVFMQSDIVDFQRQGWKPEEIMAGLCNVLPKNIWLYVSQIPNLSAIGTKFLL
                     QGGTQYNLAAVKAQVDFIESRFKGKDHPAEVIVHEHCGEAGAIGAALEAGRLWDNGRK
                     STFIGLDAVGQITFTTARSEATRCYFCKNKCLRTFIDVKTSVLSEDAYRPAKSKIPIA
                     DGAQRLIIATCEKGTVEDINEMRSIKGNLDSLKKANPNFVEIAAKAVFRVQDVPLVAD
                     PLPKLQFTASQKKRAELIKRRSEIRIGMPRVLNMYSQAPVFTGYFASLGVKAENMIFS
                     DYTSETLYKEGAKRGSIDPCFPSKLGIPHVHNLLQVHHAKKPLDLIFFPMIDCLTSDL
                     SHVQATRSCPTVAATPAAVKAAFTKEGDLFKEKNVLFLDTFVNISQPDIFERQMYEAF
                     HDVLGVSPEENARAVREGYKALDHFNNVTLRGGAREVLERLEAEDGLGIVLLGRPYHN
                     DPGVNHEILEEFQKIGYPVFPQDALPIDDDIVWRLFGDEVLSGEIPDPMAITDVWKNS
                     YSENTSRKIWAAKYVARHPNLIALELSSFKCGHDAPIYTVVEEIVEHSGTPYFCFKDI
                     DENKPTGSIKIRVETIGYFLKRYREDMIASRKKRNSIEAQLAEFEARLRRQMLREKLE
                     NLHSEEKIRESIEQGHMPTIHVSVGALAASAAQQRPAQHVSGD"
     gene            2455..3726
                     /locus_tag="VFQ79_24900"
     CDS             2455..3726
                     /locus_tag="VFQ79_24900"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEU0142983.1"
                     /translation="MEAQSNRTPCGLEERTRVVVRRVDECRSLEEVFLYSLPAFGGAF
                     LRRIYSILDRAIGMGCPLTLSFSGPVTVSGQHLAWLIPLIETGWIAYVSTTDAVCYHD
                     GHRSLDEFAEGPIHEVPIFGDDAKLLEEGVIRVTDMAFAEDVLLHQDRFLTACLMRPE
                     FQKKMTGTEMRYLLGALYAAQEQRNGVPSGLLATCHRMAVPIFVGAPADGSIFLNSMK
                     LWAMEKAGIGAEHRFEIDLHAEVFEACAYHRWGLFDNPVHALGTLILGGGVPKNYNLQ
                     PEPALGQVLGVPEVRGYMFDVQIVSAPVTDGSLSSCPPSEAVTWGKVDKEHYTKTTES
                     MQADYSTVMPFLVKALLDKRDRYARLAEEMGEERLFEKHPEARGYLRPAEGYRLFLER
                     DELTARLLKDVRENAAWLKDTLTYPLAVETP"
     gene            3763..6090
                     /locus_tag="VFQ79_24905"
     CDS             3763..6090
                     /locus_tag="VFQ79_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEU0142984.1"
                     /translation="MRTVRRVAALGAGTMGSRIAAHFANAGVEALLLDMVLPGQANRN
                     LAAMRGIETALKQKPGGFFRDTAARLVTPGNFEDDFSRLGDCDWVIEAVTENLDIKRA
                     IWSRVEQARRPDAILSTNTSGLPLAQISEGFPDSFRRNFLGTHFFNPPRYLHLVEVIP
                     GANTDPEIVAYVSGFCDKRLGKGVVLCKDTPNFIANRIGSFFGATVYKTMIEGGYTVE
                     EVDALTGPLIGLPNSASLRLVDIIGLDIWVFVARNLYDAVPEDRWRERFLPPDFVARM
                     MERGWLGDKTGQGFYKRVGKERDIHALDWRTLEYHPAGKPGFETVEKARRIASLPERL
                     RMLVAQDDRAGSFLWRLFSDVLLYSAERIPEIADRTVEIDRAMRWGYAHKLGPFELWD
                     ALGFEETARRIEREGRSLPETVTRMLSSGASSFYRHADADRRPRTEYFDFGAGRYVEL
                     EERPGILVLSDLKRARGVIKSNAAASLVDLDDGVLCLEFHSKMNTLGEETLAMLLEGL
                     EETERGVEALVIANEGQIFSAGADLNVMLAAAEAGKWDDIDRAIQRFQQAFMSLKYAA
                     KPVIAAPFGRALGGGCEVVLHAARVQASAELYMGLVEVGVGLIPAAGGTKEMLLRLND
                     AKAAFELIGFAKVSGSAADARRLGFLRDGDAITMNPERLLADAKTAALALAQSYSPPL
                     PAMEIVISGEAGYAAMKLGAWTAHEGGFLSDHDLLIADKLAHVLSGGRLSGQPKVTEQ
                     YLLDLEREAFLSLCGHPKTQERIRYMLKTGKPLRN"
     gene            6277..7452
                     /locus_tag="VFQ79_24910"
     CDS             6277..7452
                     /locus_tag="VFQ79_24910"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU0142985.1"
                     /translation="MEEVFIIDCLRTPVARAGRGALKTTRPDDTAAYVIKALLEKYPQ
                     IPREEVDDVILGCAMPEAESGMNMARIALLRAGLPDIVPGVTINRFCSSGLQSIGMAA
                     DRIRSRSASIILAGGSESMSMLPMTGNKLAPNPWVVDHRPEIYMGMGLTGEQLQRRYG
                     ITREEQDLFALRSHQHALKAQAEGHFDDEIVPVEVEETLAENGQRHARKFTFSKDEGP
                     RADTSLEALAKLKPVFHAKGTITAGNASQTSDGAAMAIVMSGTRARALGLKSKARFVS
                     FATAGVPPEIMGIGPVVAIPKALALGGLKLADIGVIELNEAFAVQALAVIRQAGLDPD
                     KVNLNGGAIALGHPLGCTGAKLTATILREMDRGHSRYGMVTMCVGGGQGAAGIFERS"
     gene            7456..>8743
                     /locus_tag="VFQ79_24915"
     CDS             7456..>8743
                     /locus_tag="VFQ79_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0142986.1"
                     /translation="MPRGGGFLLEKTAPGQVFTPEDFTEEHRAIARAADEFWRNEVAP
                     HLEAIQRQETGVAVSVLRKSAKLGLTAVLVPERYGGMELDLASAMIVAEKVARDGSYA
                     AWHGAHSGIGTIPILLFGTEEQKQAYLPRLASGELVGAYCLSESHAGSDALAARTRAT
                     LNAEGTHYVLNGQKMWITNGGAAGLFTVFAKIGGEQFTAFLVERGYPGLSTGAEEKKM
                     GIKGSSTTAVYFDNVPVPVSNVLGEIGRGHVVAFNILNLGRLKLGPFAVGGAKQTLAD
                     SLRYAKERWAFGKTIGEFGLIQHKLAEMAIRLFAAETISYRVTGDIQLRLAGFSWDKP
                     DAAATMMKTFEEFAAECSFVKVFASEMLDYVVDEGVQIHGGYGYHQDYAVERAYRDSR
                     INRIFEGTNEINRLLAAGILLKRARRGQLELVEAVKK"
BASE COUNT         1816 a         2443 c         2779 g         1705 t
ORIGIN      
        1 ggcgtcggca ccaccgggta cgcgaaagac attctgaaag atgttattgg cgcggatgcc
       61 gccgtggtgg agacggtagc tcacacgcag gcaggccttc acttctacga caacgtagac
      121 gtaatttgcg acgtcggcgg ccaggacatc aagatcatca tcctgaaaaa cggccgggtg
      181 aaagatttta agctgaacac gcagtgctcg gccgggaacg gatacttcct gcaatccacc
      241 tcgcagggct tcggcatccc ggtcgaacag tttgcggaca ccgctttcgg ggcgaacggg
      301 ttccccatgt tcggctatgg ctgcgcggtg ttcatgcagt ccgacatcgt ggattttcag
      361 cgccagggat ggaagccgga agagatcatg gccgggctct gcaacgtgct gcccaagaac
      421 atctggctat acgtttcgca aattccgaat ttgtccgcca tcggtaccaa gttcctgttg
      481 cagggcggca cgcaatacaa cctcgccgcg gtgaaggcgc aggttgattt catcgaatcc
      541 cggttcaagg gcaaggatca tcccgccgaa gtcatcgtgc atgagcactg cggagaagcc
      601 ggcgccatcg gagcggcgct cgaagccggc cgtctgtggg acaacggccg caagagcacc
      661 tttatcggtc tggatgccgt cgggcaaatt accttcacca cggccagatc cgaagccacc
      721 cgctgttact tctgcaagaa caagtgtctg cgtacgttca tcgacgtcaa aaccagcgtg
      781 ctcagcgaag acgcttaccg tcccgcaaaa tcgaagattc caatcgcgga cggggcgcag
      841 cggcttatca tcgcgacctg cgaaaagggc accgttgaag acattaacga gatgcgctcc
      901 atcaagggca atctcgacag tctcaagaaa gcaaacccga attttgtgga aatcgccgcc
      961 aaggcggtgt tccgtgtgca ggacgttccg ctggtagcgg accccctgcc gaaactccag
     1021 ttcacggcat cgcagaagaa gcgtgccgag ttgataaagc ggcgttcgga gatccgcatc
     1081 ggcatgccgc gggtgctgaa catgtactcg caggcgccgg tgttcacggg ctatttcgct
     1141 tcgcttggcg ttaaagccga gaacatgatc ttctccgact acacaagcga aacactctat
     1201 aaggaaggcg ccaagcgagg ttctatcgat ccgtgtttcc cgtcgaagct gggcattccg
     1261 cacgtgcata acctgctgca ggtgcaccac gcgaagaaac cgctcgacct gatcttcttc
     1321 ccgatgatcg attgcctcac cagcgacctg tcgcacgtgc aggcaacacg ctcgtgcccc
     1381 accgtagcgg cgactccggc agcggtgaag gccgcgttta ccaaggaagg cgatctattc
     1441 aaggaaaaga acgtcctgtt cctggatacc tttgtgaaca tctcgcagcc cgatattttc
     1501 gagcggcaga tgtacgaagc attccacgac gtgttgggcg tttcaccgga agagaatgct
     1561 cgcgcggtgc gggagggcta caaggccctg gatcacttca acaacgttac cttgcgcggc
     1621 ggcgcccgtg aggtgctcga gcggctggag gcggaagacg gcctcgggat tgtgctgctg
     1681 gggagaccct atcacaacga cccgggcgtt aaccacgaga tcctggagga attccagaag
     1741 atcggctatc ccgtattccc gcaggatgcg ctgccgatcg acgacgatat cgtctggcgg
     1801 ctgttcggcg acgaagttct ttccggcgag attcccgatc cgatggcgat caccgatgtc
     1861 tggaagaact cctacagcga gaacacaagc cgtaagatct gggctgcgaa gtacgtggcg
     1921 aggcatccga acctgatcgc attggagctt tccagcttca aatgcggcca cgatgctccg
     1981 atctatacgg tggtcgaaga gattgtcgaa cattccggta cgccgtattt ctgcttcaaa
     2041 gacatcgatg aaaacaagcc gacaggatcc atcaagatcc gcgtcgagac catcggctac
     2101 ttcctcaagc gctaccgcga agacatgatc gcgtccagga agaagcggaa cagcatcgag
     2161 gcccaactgg cggagttcga ggcacggctg cggcggcaga tgctgagaga gaaactcgaa
     2221 aatctccatt cggaagagaa gattcgagag tctatcgaac agggccatat gcccacgatc
     2281 cacgtcagtg tgggcgcgct ggcggcttcc gcggcccaac agcggcccgc gcaacacgtt
     2341 tcgggagact gaagcgggat cttaaacacg acgtcctgaa tgttcgcgcg acgatttgat
     2401 tggacacgga tgcacacaga ttctgctgct ggcatcgctt gctagaatgg cctcgtggaa
     2461 gcacaatcca accgcacgcc gtgcggtctc gaggaacgta caagagttgt agtccggcgt
     2521 gtggatgagt gccggagcct ggaggaagta tttctctatt cgctgccggc tttcggcggg
     2581 gcgttcctgc gccgcattta ttccatcctt gatcgcgcga tcggcatggg gtgtcccctt
     2641 acgttgtctt tttcaggacc ggtgaccgta tcgggccagc atctggcatg gctgattccg
     2701 ctgatcgaga ccggttggat cgcctacgtc agcaccaccg atgcggtctg ctatcacgac
     2761 gggcatcgaa gcctggacga attcgccgaa ggccccattc acgaagtccc gattttcggc
     2821 gacgatgcga agctgctcga agaaggcgtg atccgcgtta cggatatggc gttcgccgaa
     2881 gacgtccttc tccaccagga ccgctttctg accgcctgcc tgatgcggcc tgagtttcag
     2941 aagaaaatga ccggcaccga gatgcgctat cttctcggcg cgctttatgc ggcacaggaa
     3001 cagcgcaacg gagtgcccag cggcctgctc gctacctgtc atcgcatggc ggtcccgatc
     3061 ttcgtcgggg ccccggcgga tggcagcatt ttcctgaact cgatgaagct ctgggccatg
     3121 gagaaagccg gaatcggcgc agagcacaga ttcgaaatcg accttcacgc cgaagtgttc
     3181 gaggcgtgcg cgtatcatcg ctgggggctg tttgacaacc cggtgcacgc cctcggtacg
     3241 ttgattctcg gcggcggcgt gcccaagaat tacaaccttc agccggaacc tgcgctcggg
     3301 caggtgctcg gcgtccccga agttcgcggc tatatgttcg atgtgcagat cgtctccgcg
     3361 ccagtcacgg acggttcgct ctcctcctgt ccgccctcgg aagctgtcac ctggggcaaa
     3421 gtggacaagg agcactacac gaagacgacc gagagtatgc aggcggatta ttccaccgtc
     3481 atgccgttcc tcgtaaaagc gctgctcgac aagcgcgacc gttacgcgcg tctcgcggaa
     3541 gagatggggg aggagcgcct cttcgaaaag catcccgaag ccaggggcta tctgcgtccg
     3601 gccgaaggct accggctgtt tctcgagcgc gacgagctga ctgcgcggct cttgaaggac
     3661 gttcgtgaga acgccgcctg gctcaaagat acgctcacct atccgctggc agtcgaaacg
     3721 ccgtagctgc taaactcccc ccgaggggaa gcgcacaact tcatgagaac cgtcaggcgt
     3781 gtcgctgcat tgggagcagg gaccatgggc tcccgaattg cagcgcattt cgcgaacgcc
     3841 ggtgtagagg cactgctgct ggacatggtc cttcccggac aggcgaaccg caatctcgcg
     3901 gcgatgagag ggattgaaac cgccctgaag caaaagccgg gcggattctt tagggacacc
     3961 gcggcgcggc tggtcacgcc gggcaatttc gaagatgatt tttcgcggct cggcgattgt
     4021 gattgggtga ttgaggccgt cacggaaaat ctcgatatca agcgcgcgat ctggtcccgg
     4081 gtggaacagg cgcggcgtcc cgatgcgatt ctgtcgacga acaccagcgg gttgccgttg
     4141 gcgcagattt cggaaggctt tccggattcg ttccggagaa attttctggg gacgcacttc
     4201 ttcaacccgc cgcgctacct gcacctggtg gaagtgattc ccggcgcgaa cacggacccg
     4261 gagatcgttg cctatgtctc cggcttctgc gataagcggc tgggcaaagg cgtcgtgctt
     4321 tgtaaggaca cgccgaactt catcgccaac cggattggaa gctttttcgg agcgacggtc
     4381 tacaagacga tgatcgaagg cggctatacg gttgaggaag tcgatgcgct cacggggccg
     4441 ctgatcggac ttccgaacag cgccagtctg aggcttgtcg atatcatcgg gctcgacatc
     4501 tgggtttttg tcgcgcgcaa tttatacgac gcggttcccg aagatcgttg gagagagcgg
     4561 ttcctgccgc cggatttcgt ggcgcggatg atggagcgcg gctggctcgg agataaaaca
     4621 ggacagggtt tctacaagcg agtgggcaag gagcgcgaca tccatgcgct cgactggcga
     4681 acgcttgaat accatccggc tggtaaaccg ggttttgaaa ccgtggagaa agcgcgccgc
     4741 atcgcgagcc tgccggaacg gttgcgcatg ttggtggcgc aggacgatcg cgcgggctcg
     4801 tttctatgga gactcttcag cgacgtcctg ctctattcgg cggagcgaat cccggaaatc
     4861 gcggaccgca ccgtcgagat cgaccgtgcg atgcgatggg gttatgcgca caagctggga
     4921 cccttcgaat tgtgggatgc gctcggtttt gaagagactg cccggcgcat agagcgcgag
     4981 ggccgctcgc tgccggaaac cgtaacgcgg atgctctcct ccggcgcgtc gtcgttttac
     5041 aggcacgccg atgcggatcg caggccacgc acggagtatt tcgattttgg cgccggccgc
     5101 tatgtagagc tcgaagagag gcccggtatt ctggtgctct ccgatctgaa gcgcgcgcgg
     5161 ggagtcataa agagcaacgc agcggcatcg ctggtggacc tggacgacgg tgtgctgtgt
     5221 ctcgaatttc acagcaagat gaacacgctt ggcgaagaga ccctcgccat gctgctcgaa
     5281 ggactggagg agacggagcg tggcgttgaa gcgctggtga ttgcgaacga gggccaaatc
     5341 ttcagtgcgg gtgcggatct gaatgtgatg cttgctgccg ccgaagcagg caagtgggat
     5401 gacatcgacc gcgccatcca gcgctttcag caggcgttca tgtccttgaa gtacgcggca
     5461 aagcccgtca tcgcggcgcc gttcgggcgc gccctgggcg gcggatgtga agtggttctt
     5521 cacgccgcgc gcgtacaggc atcggctgag ctgtacatgg gactggtaga agtgggggta
     5581 ggtttgattc cagcggccgg tggaacgaag gagatgctgc tgcggctcaa tgacgcgaaa
     5641 gcggcattcg agctcattgg ctttgcgaag gtatcgggca gcgccgccga tgccaggcgg
     5701 cttggtttcc tgcgcgacgg tgacgcgatc acgatgaacc ccgaaaggtt gttggcggat
     5761 gccaaaacgg cggctctcgc gctagcccaa agttattcac cacctttacc cgctatggag
     5821 atcgtcatct ccggagaagc cggatatgcg gcgatgaaac tgggagcatg gacggcgcac
     5881 gaaggcggct tcctcagcga tcatgacctg ttgattgcgg acaaactggc gcatgtgctc
     5941 agcggcggcc ggctcagcgg acagccaaaa gtaacggagc agtatctgct cgatctcgaa
     6001 agggaagctt ttctgagcct ctgcggccat ccgaagacgc aggaacgaat ccggtacatg
     6061 ctgaagacgg gaaagcccct gcgaaattaa acacgacgtt ctaaatgttt gtgcgacgat
     6121 ttgattggcc acggatggac acagatgaac acagataaga acaatgcaag gcttgatccg
     6181 tgtgaatccg tgttcatccg tggccgaatt tgcttttctg gattgctgag atgcgcaatt
     6241 atttatgacg ttctgtttag aaagaggcgt tgagacatgg aagaggtatt tatcatcgat
     6301 tgtcttcgga cgcccgtcgc gagagcgggc cggggtgcgc tgaaaaccac ccgtccggac
     6361 gacacggcgg cgtacgtcat caaggcgctg ctcgaaaagt atccgcagat cccgcgcgaa
     6421 gaagtggatg acgtcattct cggctgcgcg atgcccgaag ccgaatccgg catgaacatg
     6481 gcgcgtatcg ctctgctgcg cgccggactg ccggacatcg ttcccggtgt gacgatcaac
     6541 cgtttctgtt cttccgggct gcaatccatc gggatggcgg cggaccgcat tcgctcccgt
     6601 tcggcgagca tcatccttgc gggaggaagc gaatcgatga gcatgctgcc catgacggga
     6661 aacaagctgg ctccgaatcc ctgggttgtg gatcatcgtc cggagattta catgggcatg
     6721 gggctcaccg gcgagcagtt gcagcgccgc tacggcatca cgcgcgagga gcaggatttg
     6781 ttcgccctgc gcagccatca gcatgctttg aaggcgcagg ccgaaggcca cttcgatgac
     6841 gagattgtgc ctgtcgaagt ggaggagacg ctcgccgaaa acgggcagcg gcacgcgcgc
     6901 aaattcacgt tctccaagga tgaaggtccc cgggcggata cgtcgctcga agcgttggcg
     6961 aagttgaagc cggtgttcca cgcgaaaggc accattacgg ccgggaacgc ctcgcagacc
     7021 agcgatggcg cggccatggc gatcgtcatg tcgggaaccc gcgcccgtgc cttggggctg
     7081 aagtccaagg cacgatttgt gagctttgcg acggcggggg ttcctccgga gattatgggc
     7141 atcgggccgg ttgtagccat accgaaagcg ttggcgctcg gcggattgaa gctcgcggac
     7201 atcggcgtta tcgaattgaa cgaagcgttc gcggtacagg cgttggccgt gattcgacag
     7261 gccggtctcg atccggacaa ggtgaatttg aacggcggtg cgattgcgct gggtcatccg
     7321 ttagggtgta ccggcgcgaa gctgacggcg accattctgc gggagatgga ccgcgggcac
     7381 tcgcgctatg gcatggtgac gatgtgcgtg ggcggtggac agggcgccgc cggtatcttc
     7441 gagaggagct agcggatgcc gcgcggcgga gggttcctgc tggagaagac ggcgcccggc
     7501 caggtgttca cgccggaaga cttcaccgag gagcatcgcg ccatcgcccg cgccgcggat
     7561 gagttctggc gcaacgaagt ggcgccgcat cttgaagcga ttcagcgaca ggagacgggt
     7621 gtcgcggtat cggtactgcg taagtcggcg aagcttggcc tgaccgccgt gctggtcccg
     7681 gagcgctacg gcggcatgga gcttgacctg gcttcggcga tgattgttgc cgaaaaggta
     7741 gcgcgagacg gctcctatgc ggcctggcac ggggcgcaca gcggcattgg aacgatccct
     7801 attctgctgt ttggcaccga ggagcagaag caggcgtacc tgcccaggct cgcgagcggc
     7861 gaactggtag gcgcctactg cctgagcgaa tcccatgcag gctccgacgc cctggccgcg
     7921 cgaacacgag ccacgctgaa tgccgaaggt acgcactatg tgctgaacgg gcagaagatg
     7981 tggatcacca acggtggagc tgccggcctg ttcacggttt tcgcgaagat cggcggcgag
     8041 cagtttacgg cgttcctggt agagcgcggc tatccgggcc tcagcacggg cgccgaagaa
     8101 aagaagatgg gcatcaaggg cagctccacc accgctgtct acttcgacaa tgttccggtc
     8161 cctgtttcca acgtgcttgg agaaatcggg cgcgggcacg ttgtcgcatt caatattctg
     8221 aacctgggac gtctgaaact cggaccgttt gccgtaggcg gcgccaagca gacgctcgct
     8281 gattcgttgc ggtacgcgaa agagcggtgg gcttttggaa aaacaatcgg cgagttcggg
     8341 ctcatccagc acaagctggc tgaaatggcc attcgcctct ttgccgccga gaccatcagt
     8401 taccgcgtca cgggcgacat tcagttgcgg ctggccgggt tctcctggga taagccggac
     8461 gccgccgcca cgatgatgaa aactttcgag gagttcgccg cggagtgttc gttcgtgaag
     8521 gtgttcgcgt ccgagatgct cgactatgtt gtggatgaag gtgtccaaat ccacggaggc
     8581 tacggctatc accaggacta cgcggtggag cgcgcctacc gcgattccag aattaatcga
     8641 atcttcgagg gcacgaacga gatcaaccgg cttctcgccg ccggcattct gctgaagcgt
     8701 gcccggcgcg ggcaactcga gctggtggaa gcagtgaaga agt
//
LOCUS       DASTMK010000604        14446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_446615, whole genome shotgun sequence.
ACCESSION   DASTMK010000604 DASTMK010000000
VERSION     DASTMK010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446615"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFQ79_24920"
     CDS             complement(<1..279)
                     /locus_tag="VFQ79_24920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142987.1"
                     /translation="MRIAAAFSRHARVADAVDRMIAMQIGGLHDRGNVFKSCDHRGGI
                     GVITGPEKASNVRLLHVRGKRVLAIAGVPVSLETPLAPLLDRAIELPFD"
     gene            complement(307..2220)
                     /gene="glgB"
                     /locus_tag="VFQ79_24925"
     CDS             complement(307..2220)
                     /gene="glgB"
                     /locus_tag="VFQ79_24925"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU0142988.1"
                     /translation="MNSLLTADDLHLFNEGTHLRMYNKLGCHLTSMDGAEGAYFAVWA
                     PNAAEVSVIGDFNGWEKARDRLSLRADSGIWEGFLPGVTQGACYKFHLVSRHHGYRVD
                     KADPYAKYFQTPPERAAIAWNLDYQWGDGEWMAARHSRNSLTSPISIYEVHTGSWRRV
                     PEEGNRPLTYREMAAPLAEHVLHTGFTHVEFLPVMEHPFYGSWGYQTTGYFAPASRYG
                     TPQDFMWLIDYLHQRGIGVILDWVPSHFPNDEHGLGYFDGTHLYEHSDPRQGVHRGWD
                     TFVFNYARNEVRTFLESSAMFWLDKYHIDGLRVDAVASMLYLDYGRRDGEWIPNPYGG
                     RENLDAIAFLRTLNEKIYQEYPGVQTIAEESTAWPMVSRPTYAGGLGFGMKWDLGWMH
                     DTLEYMSKVPLFRKHHHDRLTFRGMYAFSENFVLPLSHDEVVYGKGSLLSKMPGDDWQ
                     KFANLRLLLGYMYAIPGKKLLFMGGEFGQWNEWYHEASLDWHLLEDERHPKLQNWVRE
                     LNLFYRGEPTMHLFDLEPQSFEWVEVHDTENSVIAFLRRGRSAEENILVACNFTPVTR
                     YNYRIGVPEKGFWSERLNSDAVEHGGSGQGNLGGVNTVPVPVHGHPQSLTLTLPPLAA
                     LFLHYSLFNTNET"
     gene            complement(2217..5555)
                     /gene="treS"
                     /locus_tag="VFQ79_24930"
     CDS             complement(2217..5555)
                     /gene="treS"
                     /locus_tag="VFQ79_24930"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEU0142989.1"
                     /translation="MTKRTQQLEEGEALWYKDAIIYELHVRAFKDYSGDGAGDFRGLT
                     EKLDYLQDLGVTAIWLLPFCPSPWRDDGYDISDYTDVHPAYGTLRDFQHFLDAAHARG
                     LRVITELVVNHTSDQHAWFQRARKSPPGSKWRDFYVWSDTPEKYKEARIIFKDFETSN
                     WTWDPVAQAYFWHRFYSHQPDLNYDSPHVRKAVIEAMEFWFDMGVDGLRLDAVPYLYE
                     REGTNCENLPETHQFLKELRAHIDSKYSDKLLLAEANQWPEDSVAYFGEGDECHMAFH
                     FPLMPRMFMAQRMEESFPIIDILDLTPPIPDNAQWALFLRNHDELTLEMVTDEERDYM
                     YRVYATDPQARINLGIRRRLAPLLENSRRRIELMKGLLFSLPGTPVIYYGDEIGMGDN
                     VYQGDRNGVRTPMQWNPDRNAGFSTANPQQLYLPLVIDPEYHYESVNVETQQNNPYSL
                     LWWVKSMIAQRKRWKALSRGAIEFLNPDNRRVLAFVRCYQEEHILVVANLSRFPQPVE
                     LDLSKFEGRVPVEVFGMVEFPQIRDSSYLVTLAPQAFYWFHLEPARPDRESLESVSDP
                     SQLPLMTVKSFHHALDSSGRQVLRRLLPSFLPTRRWFRSKARKIRSLDIVDTIPFDNT
                     GATFVLIRVEYNSGDPELYVVPLSVATGEDRDDILKNNPAIVVARLRDSSGEEGVLYG
                     AFRSQAFCEGLLQALQRKRRFKGEAGDLVTTPTRLFRDLMGDDACLAPSVQRSEQSNT
                     SVVYGDRLILKLFRRIEEGVNPDLEIGRYLTEKTKFQNIAPVAGWIEYQRNGTKMTAA
                     ILQAYVPNQGDAWSYSIDALSRYFPRALARGTQGTAPPDLPSNPLDIEEIPESSMELL
                     ESYAEDARLLGQRTAEMHLALTQPTGDAALSPEPFTDFYQHGLYHGMIALARRSLQLL
                     RQQLRQLPEDVRVPARQVLDMEKQVVQRFRPLRDQRVAALRCRHHGDYHLGQLLYTGR
                     DFVIIDFEGEPLRSISERRIKRSPLRDVAGMLRSFHYVSSAALFGQVPGIVVEERLHD
                     ELRRWARFWYLASSHNFLKGYLETAGSALFLPKQKDHQHILLDSYTLEKAMYELGYEL
                     NNRPDWVRIPLQGILDLLGTGETK"
     gene            complement(5564..7540)
                     /locus_tag="VFQ79_24935"
     CDS             complement(5564..7540)
                     /locus_tag="VFQ79_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEU0142990.1"
                     /translation="MPADDGRKRVVIEGVSPEIDAGRFPVKRTVGESVTIEADIFTDG
                     HDALAAVLLYRKEEDETWTEVPMSFLLNDRWSAQFQVDKIGKYHYTLQAWVDTFRTWR
                     RDMVKRIRAATDAEVDYLVGVQLVEDAAGRATGPDRNRLQRWAEALRTGGRAAALDEE
                     MSLLMARYPDRRFQTIYPKELAVTVDRERARYSAWYEFFPRSVSPEPGRHGTLKDATA
                     HLSYVREMGFDVVYFPPIHPIGRTYRKGRNNSVTPKPDDVGSPWAIGAEEGGHKSIHP
                     ELGTIEDFRKLMAEAKRLGLEVALDIAFQCSPDHPYVKEHPSWFRQRPDGTVQYAENP
                     PKKYQDIYPFDFETPDWCELWNELKSVLFFWIGEGVRIFRVDNPHTKPYAFWEWVVGE
                     IKNAHPDVIFLSEAFTRPKVMYALAKLGFTQSYTYFAWRNTKWELERYFTELTKTQVR
                     EFLRPNLWPNTPDILTEYLQFGGRPAAVIRLVLAATLGASYGIYGPAFELFENRPREP
                     GSEEYLDSEKYQLRHWDLNRPDSLRDFIARVNSIRRANPALHRDWSLRFHRTDNENIV
                     CYSKRTEDSSNIVLVAVNLDPHHTQSGWIELDPEEIPSETHRPYQVHDLLSEARYLWQ
                     GQRNYVELDPKIVPAHIFTIRRRIRTERDFDYYM"
     gene            7837..8253
                     /locus_tag="VFQ79_24940"
     CDS             7837..8253
                     /locus_tag="VFQ79_24940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142991.1"
                     /translation="MQARMEVALAQERAASADLRASVTQHERIAEERVKAAEEAAQRR
                     ALDQLLEEFRVEERHYVRESKSLFLNRKSMVVQERLFFRSIPLSNWIEHEMVVEEGGD
                     LQQLAQSCSVFVPQAAREVAAAGPRLIRSAGAKLGA"
     gene            8292..9215
                     /gene="trxB"
                     /locus_tag="VFQ79_24945"
     CDS             8292..9215
                     /gene="trxB"
                     /locus_tag="VFQ79_24945"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU0142992.1"
                     /translation="MRNVVIVGSGCAGNTAAIYTARANLKPLVIAGHEPGGQLSLTTL
                     VENFPGFPEGINGPDLVENMKKQAQNFGAEYIHGSVIEADLSKRPFRLNVEGEWLETR
                     TLIIASGASARWLGLESEQKLIGHGVSSCATCDGFFYRDKQIIVVGGGDSAMEEATFL
                     TRFGREVALVHRRDEFRASKIMLDRARANPKIRFLLNTVVEDVYDIEKGYVTAVKLRN
                     VQTGELSEREVDGLFVAIGHIPNTAAFKGQIELDEDGYIKSRGNARTSVTGVFHAGDV
                     EDRVYRQAITASGAGCMAAMEAERFLESEGH"
     gene            9276..9605
                     /locus_tag="VFQ79_24950"
     CDS             9276..9605
                     /locus_tag="VFQ79_24950"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU0142993.1"
                     /translation="MFCTHCGTELDGICRFCHQCGTATTKQVPRPPAASPGLSIPLEG
                     RKIGGVCAGFARYIGVDVTFLRIFWLTLAIFTGVGFVAYLIAWLLMPKERYLLPAPST
                     ETAGQTV"
     gene            9674..10828
                     /locus_tag="VFQ79_24955"
     CDS             9674..10828
                     /locus_tag="VFQ79_24955"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEU0142994.1"
                     /translation="MPARTESPKARLAVIEFRAKLIGDPVKRLRFLRRVVNTSDFGDS
                     PASNWRLRALLVAIIVIPLLIDTSPAVISAELRTPAISPLVGSYDPLPVWLVERGAEH
                     ETYSNGLRIENAFRVATHKRSYQIFDREYPNRIAARTKTDPAGIVFHTSESTQLEFEE
                     ERNGALQRIGESLLGYVRARRSYNFLIDRFGRTFRIVPESDVANHAGHSIWADAKYIY
                     LNLNSSFIGVSFEAETQVGEQPSVNPAQIHAGRMLAAMLRSQYGIAVENCVTHAQVSV
                     NPANMRIGYHTDWAANFPFAAMGLPDNYALALPSIAAFGFTYDATFLRSTGHSLWRGL
                     AAGEELLRQSAASRGVPAAEYRKALQRQYQERISALRRLGVQEETSNEAN"
     gene            10815..11819
                     /locus_tag="VFQ79_24960"
     CDS             10815..11819
                     /locus_tag="VFQ79_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0142995.1"
                     /translation="MKQTEGKAIPIGLDIGTSRIVLARHVDKEFHFASQLNAFVRIPF
                     SKITEDVLRKEQIPYAADGSVLFVHGDASQRFADLLKSEISRPMDRGFLNPSEPDGLR
                     LIREIVSSMTGKSDTRRKLYFTVPAAPAGAEENFTYHEAALRQELEDLGFEARSLNEG
                     LAVVYSELEASNFTGIGVSCGGGLCNVCLSYLSVPITSFSLPKAGDFIDTSAASVTGE
                     RANRIRILKEESLSLNNGHMKDKVQRVLSVYYDDMIRSLASALKETFANVRGLPEFGK
                     PIPLVLAGGTAMPKGFRDRFEAALRETGFPIPLSEIRLASDPLSATAKGALVAALSEM
                     "
     gene            complement(11823..12692)
                     /locus_tag="VFQ79_24965"
     CDS             complement(11823..12692)
                     /locus_tag="VFQ79_24965"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0142996.1"
                     /translation="MADRRHSRRLTVGLVVVVIAAIGTAVWLATSRLNQLGRQLEEAE
                     TRAGNVEKQLARYSDEVTDAIQRAEAAKKREAEAESAASAAEQDRTAAQLQAEQAQQA
                     AQEAQQQATTAHQRAEAAAAAEQQTRSELETIRKRREDELNRMQEALNRIAPTRRTPA
                     GMVMELADDSFHFDFDKASLRPENREVLSRIAGVLLASEGYRLFVYGHTDDVGTREYN
                     QNLSERRAQTIRDYLVQAGIPSEIITTQGFGKSSPAVKATNSNARAKNRRVELGIVDT
                     IINYQGQVPPPKG"
     gene            complement(12741..13721)
                     /locus_tag="VFQ79_24970"
     CDS             complement(12741..13721)
                     /locus_tag="VFQ79_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU0142997.1"
                     /translation="MKGLTDIPGIRVGHSSDYDALTGCTVILCEDGAVAGYDIRGGAT
                     GTEEFEVMSPLHVTDHIHALVFAGGSAFGLEAASGVRRYLEQKGIGYTTRAAKVPLVP
                     CAILYDLTIGKAGVRPTREMGEAAAAAAHTGVVEEGAVGAGTGATTGKIFGIARAMKS
                     GLGTFTVALGGSHTDVLVSSLTVVNAYGDVIDPQTGKLLAGARASEESLELVGTAAAM
                     KAVRQAPARHENTTLAVVATNARLSKVQATRLAQLASVGIARTISPVWTSYDGDLVVA
                     LSTGTRDADVNALGVAAVEAVAESVQRAVRLSPGLGGLPGLASARPGGLR"
     gene            13766..>14446
                     /locus_tag="VFQ79_24975"
     CDS             13766..>14446
                     /locus_tag="VFQ79_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0142998.1"
                     /translation="MLDILKPPVLALQDFYSLAGRAIRNIVRRPHYYDDIVLQMDVIG
                     VGSLPIVLLTGFFSGAVMALQMSRALSQYGATGQVGMIVSITLVRELGPVLTAVVVAG
                     RNASGMASELGSMKVTEQIDAMRALGTDPVQKLVTPRLIATAVVLPLLTIVADFVGLV
                     GGYVVAVPLLSLTTAGEYWNTAWRAIEYNDIAQGLLKPLVFAIIIALVGCFYGMRTTG
                     GTQGVGRAT"
BASE COUNT         2832 a         4264 c         4262 g         3088 t
ORIGIN      
        1 atcgaacgga agctctattg cccgatccag cagcggcgct aacggcgttt cgaggctcac
       61 cgggacgccg gctatggcca ggacacgctt tccacgtaca tgcaggagac ggacgttgga
      121 agccttctcg gggccggtga ttacgccaat gcctccgcga tggtcacaag atttgaatac
      181 gttgccgcgg tcgtgcagcc ccccgatctg catggcgatc atgcggtcta ctgcgtcggc
      241 tactcgcgcg tgacggctga aggccgctgc gattcgcatg cttacgacaa tattaatcta
      301 tttcaattaa gtttcgtttg tattaaataa cgaataatgc aggaacaggg ccgccagcgg
      361 cggcaatgtc agggtgagcg attgaggatg cccatggacg gggaccggaa ccgtattcac
      421 tccacccaaa tttccctggc cgctgccgcc gtgttccacc gcgtcgctgt tcaggcgttc
      481 gctccagaaa cccttctccg gcactccaat ccgatagttg tagcgggtta cgggagtaaa
      541 gttgcacgcc accagaatgt tttcctccgc ggacctaccc cgccgcagga aagcgatgac
      601 gctattttcg gtatcgtgaa cctccaccca ctcaaagctc tgcggttcga ggtcgaacag
      661 gtgcatcgtc ggctccccgc gatagaacag gttcagttca cggacccagt tctgtagctt
      721 cggatgccgc tcgtcctcaa gaaggtgcca gtcaaggctt gcctcgtggt accactcatt
      781 ccactgtccg aactcccctc ccatgaacag cagtttcttt cccggtatcg cgtacatata
      841 gcccaacagg aggcgtagat tggcgaactt ctgccagtcg tcgccgggca tcttgctgag
      901 cagcgaccct tttccgtaaa ccacctcatc atgcgacaag ggcagtacaa agttctccga
      961 gaaagcgtac atgccgcgaa aggtgagccg gtcatggtgg tgtttacgga agagcggaac
     1021 cttggacatg tactccagcg tgtcgtgcat ccatcccagg tcccatttca ttccgaaccc
     1081 gagcccgccg gcatacgtcg ggcgtgaaac catcggccat gcggtggatt cttcggcgat
     1141 cgtctgcacg ccgggatatt cctggtatat cttctcgttg agggttcgga gaaaggcgat
     1201 cgcgtccagg ttctccctac ccccatatgg attgggaatc cattccccat cccggcggcc
     1261 gtaatccaga tacagcatgg acgcgacggc gtccacccgc aagccgtcga tgtggtactt
     1321 gtccaaccag aacatcgcac tgctttcgag aaaggtgcgc acttcattgc gggcgtagtt
     1381 gaagacgaat gtgtcccagc cgcgatggac gccttgtctt gggtccgaat gctcgtacag
     1441 gtgagtgccg tcgaagtacc cgaggccatg ttcatcattg gggaagtgcg aggggaccca
     1501 atccaggatt accccgatgc ctcgctggtg caggtaatcg atcagccaca tgaaatcctg
     1561 cggcgttccg taacggctgg ccggtgcgaa atagccggtc gtctgatagc cccaggaacc
     1621 gtaaaaaggg tgctccataa ccggcagaaa ctcgacgtga gtgaagcccg tgtgtaatac
     1681 gtgctccgcg agcggcgcgg ccatttcgcg ataggtcagc gggcggtttc cctcttcggg
     1741 gacgcggcgc caggaaccgg tatgaacctc gtagatagag atggggctcg tcagggaatt
     1801 ccggctgtgc cgcgcggcca tccactcgcc atcgccccat tgataatcga ggttccaggc
     1861 gatggcggca cgttcgggcg gtgtctggaa atacttggcg taagggtccg ccttgtccac
     1921 acgataaccg tggtgtctgg agacgaggtg gaatttgtag caggcgcctt gcgtcacccc
     1981 gggaaggaag ccttcccata tgccggaatc cgcccgcagg ctcaaccggt cgcgggcctt
     2041 ctcccaacca ttgaagtcgc caatgacgga gacttccgcc gcgttgggcg cccaaaccgc
     2101 aaaatacgcc ccctcggcgc cgtccatcga cgtaaggtgg cacccgagtt tgttgtacat
     2161 ccggagatga gtgccttcgt tgaagaggtg cagatcgtct gccgtcagaa ggctgttcat
     2221 ttcgtttccc cggtcccgag cagatcgaga atgccctgaa gggggatgcg gacccaatcc
     2281 ggacgattgt tcaactcgta ccccagctcg tacattgctt tttcgagtgt gtaggaatcg
     2341 agcaggatgt gttgatggtc tttttgtttc ggcaggaata atgcgctgcc ggcggtttcc
     2401 aggtaaccct tcaggaaatt gtgggaagaa gccaggtacc agaagcgcgc ccagcggcgc
     2461 agttcgtcgt gcaagcgctc ctcaaccaca ataccgggga cctgcccgaa cagcgccgcc
     2521 gaagagacgt agtggaaaga ccgcagcata cccgccacgt cgcgcaaggg cgagcgcttg
     2581 atgcggcgct cactgatgga acgcagcggt tcgccttcaa aatcgatgat gacgaaatca
     2641 cggccggtat agagtaactg gcccaaatga tagtcgccgt ggtggcggca gcgcagggct
     2701 gctacccgct ggtcgcgcag gggacggaag cgctgcacca cctgtttttc catgtccagg
     2761 acctgcctgg cagggacccg cacatcttcg ggcaattgcc tcagttgctg gcgtaacaac
     2821 tgcaaggacc ggcgcgccag cgcgatcatg ccgtgataca ggccgtgttg atagaagtcg
     2881 gtgaacggct ccggagagag agccgcgtcg ccggtcggtt gggtcagggc aaggtgcatc
     2941 tccgccgttc tctgtccaag cagccgggca tcttcggcat acgattccag cagttccatc
     3001 gaggattcgg ggatctcctc gatatccagc gggtttgatg gcagatcggg gggcgccgtt
     3061 ccctgtgttc cgcgcgccag cgcacgtgga aaatagcggc tcaaggcgtc aatggaatac
     3121 gaccatgcgt ctccctgatt cgggacatag gcctgaagaa tggcagccgt catcttcgtc
     3181 ccgttgcgtt gatactcgat ccaacccgca acgggagcga tattttggaa tttcgtcttc
     3241 tctgtcagat agcgcccgat ttcgagatcg gggttcaccc cttcttcaat acgccggaac
     3301 agcttcagga tgagacgatc tccgtaaacc accgaagtgt tgctctgttc cgaccgctgg
     3361 acggagggcg ccaggcaggc atcgtctccc atcagatccc ggaacagccg ggtgggtgtt
     3421 gtgaccagat cgccggcctc tcccttaaac cgcctcttgc gttgaagagc ctgaagcagt
     3481 ccctcacaga atgcctggct gcggaatgcg ccatacagaa cgccctcctc acctgagctg
     3541 tcccggagcc tggccacgac aatggcggga ttgttcttca ggatgtcgtc gcgatcttcc
     3601 ccggtggcga cggaaagtgg cacgacatac aactccgggt cgccggaatt gtactcgacc
     3661 cggatgagta cgaatgtggc gccggtgttg tcgaacggaa tggtatcgac gatgtcgagc
     3721 gaacggattt ttcgcgcttt gctccggaac cagcgcctgg tagggagaaa tgagggaagc
     3781 aatcgtctca gaacctgccg tccggagcta tccagcgcgt gatggaagga tttcaccgtc
     3841 atcaacggca actgggaagg gtcgctgacc gattccaacg attcgcgatc gggcctggcg
     3901 ggttcgagat ggaaccagta gaaagcctgg ggagcaagcg tgacgagata gctggaatcc
     3961 cggatctggg ggaattccac cattccgaac acctcgaccg gcacccggcc ctcaaacttc
     4021 gacaggtcca gctctaccgg ctgcgggaaa cgcgacaggt tcgccaccac cagaatatgt
     4081 tcctcctgat agcagcgaac gaatgccagg accctgcgat tgtccgggtt caggaactca
     4141 atggcgcctc gcgataacgc tttccagcgc ttccgctgcg caatcatact tttcacccac
     4201 cagagcaggg aatacgggtt attctgctgc gtctccacat tcacggattc atagtgatac
     4261 tcggggtcga tgacgagcgg aagataaagc tgctgcggat tggccgtcga gaagcccgcg
     4321 ttgcgatccg gattccactg catgggtgtg cgaacgccgt tccggtcgcc ctggtagacg
     4381 ttgtcgccca ttccgatctc atcgccgtaa tagataacgg gagtgcccgg cagagagaac
     4441 agcaggccct tcatgagctc gatgcgccgg cggctgtttt cgagcagcgg cgccaggcgg
     4501 cgccggattc caaggttgat ccgggcctgc gggtcggtcg catagacgcg gtacatgtag
     4561 tcgcgttctt cgtccgtcac catctccagt gtcagttcgt cgtgattgcg cagaaacaag
     4621 gcccactgcg cgttgtccgg aataggcggc gtgagatcca ggatgtcaat gatcgggaag
     4681 ctttcttcca tccgctgcgc catgaacatg cggggcatca gcgggaaatg aaacgccatg
     4741 tggcattcgt cgccctcgcc gaaataggcc accgagtctt caggccactg gtttgcttcg
     4801 gcaagcagca gtttgtccga atacttcgag tcgatgtgcg cccgaagctc cttcagaaac
     4861 tggtgcgtct ccggaaggtt ctcgcagttt gttccttccc gttcgtacaa gtagggcacc
     4921 gcatccagcc gcagcccgtc cactcccatg tcgaaccaga actccatcgc ctcgatgacg
     4981 gctttgcgga cgtgaggact gtcatagttc aggtcgggct gatgcgaata gaaccggtgc
     5041 cagaaatagg cttgcgccac cgggtcccag gtccagttcg aagtctcgaa atctttgaag
     5101 atgatgcggg cttctttata cttttccggc gtatcgctcc aaacgtagaa gtcgcgccac
     5161 ttgctgcccg gcggcgactt tctcgcccgt tgaaaccacg cgtgctggtc ggaagtatgg
     5221 tttacgacca actccgtgat cacccggagc ccgcgcgcat gcgccgcgtc cagaaagtgc
     5281 tggaagtccc gcagcgtccc gtaagcggga tgtacgtccg tgtaatcgga aatatcgtaa
     5341 ccatcgtccc gccagggcga cgggcagaaa ggcagcagcc atatagccgt cactcccagg
     5401 tcctgaagat agtcgagctt ctcggtcagg ccgcgaaagt ctcccgcccc gtcgccgctg
     5461 taatccttga aggcccgtac gtgtaactcg taaatgatgg cgtctttgta ccaaagcgcc
     5521 tcgccttcct ctagttgttg ggttctcttc gtcatagcgc tgcttacatg taatagtcga
     5581 aatcccgctc cgtgcgaatt cgccgccgaa tggtgaaaat gtgcgcaggc acgatctttg
     5641 gatccagttc aacgtagttc cgctggccct gccacagata acgcgcctcg ctcagcaggt
     5701 cgtgaacctg gtatggcctg tgggtttcgg agggaatctc ctccgggtcg agttcaatcc
     5761 agccggactg tgtgtgatgc gggtccaggt tcacggccac cagaacaatg ttgctggagt
     5821 cctctgtccg tttgctgtag cagacgatat tttcgttgtc cgtccggtgg aagcggaggc
     5881 tccagtcgcg gtgcagggca ggattggccc gccggatcga gttcacacgc gcaatgaagt
     5941 cgcgcaggct gtccgggcga ttcaagtccc agtgccggag ttgatacttt tccgaatcga
     6001 ggtactcttc gcttccaggc tcgcggggcc ggttttcgaa caactcgaag gcaggcccgt
     6061 agattccata gctcgcgccc agcgtggcgg caagcaccag cctgatgacg gcggccggcc
     6121 gccccccaaa ctgaagatat tcggtgagaa tatcgggcgt gttaggccat agattcggcc
     6181 ggagaaactc gcgtacctgc gtcttcgtca gctctgtgaa atagcgctcc aactcccatt
     6241 tcgtattccg ccacgcgaaa taggtgtagg actgtgtgaa gccgagcttc gccagggcat
     6301 acatcacctt gggccgggtg aaagcctccg aaagaaagat cacatcgggg tgcgcgttct
     6361 tgatttcgcc aaccacccac tcccagaaag cgtacggctt ggtgtgcgga ttgtcgacgc
     6421 ggaaaatgcg gacgccctca ccgatccaaa agaagagcac gcttttcaac tcgttccaaa
     6481 gttcgcacca atccggcgtt tcgaaatcga aaggatagat atcctggtat ttcttgggtg
     6541 gattttcggc gtactgcacg gtgccgtccg gccgctggcg aaaccagctc gggtgttcct
     6601 tgacgtaggg atgatccggc gagcattgaa aagcgatgtc gagcgcaacc tcgaggccca
     6661 gccgcttcgc ttccgccatc agcttgcgaa aatcctcgat ggttccgagc tccggatgga
     6721 tggatttatg accgccttct tccgctccga tggcccatgg gctccccacg tcgtccggct
     6781 taggggtaac agagttgttc ctgccttttc ggtacgtccg cccgatagga tgaattggag
     6841 gaaaatagac tacatcaaag cccatttcgc ggacgtagga caagtgggcc gtggcgtcct
     6901 tcagcgtgcc atgacggccc ggctccggcg aaaccgatcg agggaagaat tcgtaccatg
     6961 cgctgtagcg cgcccgttcg cgatccactg ttaccgcaag ttccttggga tagatggtct
     7021 gaaaccggcg gtccggatag cgggccatta acagggacat ctcctcgtcg agcgcggcgg
     7081 cgcggccgcc ggtgcgcagg gcttcagccc atctttgaag gcggttccgg tccggtccgg
     7141 tagccctgcc ggcggcatct tccaccaact gtacacccac caggtaatcc acctcggcgt
     7201 cagttgcggc acgaatgcgt ttgaccatat cgcgccgcca ggttcgaaac gtatccaccc
     7261 aggcctgaag ggtatagtgg tacttcccaa tcttatccac ctggaactgc gcgctccatc
     7321 tgtcatttaa caggaaggac atcgggactt cggtccaggt ttcgtcttct tcttttctgt
     7381 agaggagaac cgcagcgagg gcatcatggc cgtcggtgaa gatgtcggcc tcaatcgtta
     7441 cactctctcc cacggttctt ttgacaggaa agcgcccggc gtcgatttcc gggctaactc
     7501 cttcgattac tacgcgttta cgtccgtcat ctgccggcat ttattctctt tcttcgatcc
     7561 agcttaggag ctttttgaac gcgttgcagc cacgctccct gacggtcgcg gctcagaaac
     7621 ggagccgcgc gcgtcagcaa gcggtttttc cacgtttccc cacactctta gacgcccaaa
     7681 atcccgagac ttagcacgaa gccatgaaaa cgttccaggg cagctaatgt tcccgccgga
     7741 accggccgat agaccatcgg gactatggca agcttcctgt ggattttcct cccgatattt
     7801 gtagccaccg gttctgcgct gctcgcgttc tgtcttatgc aagcacgcat ggaggtggct
     7861 ttggctcagg agcgtgcggc atcggccgat ctgcgcgctt ccgtcacgca gcatgagcgg
     7921 attgcggaag agcgggtcaa ggctgccgaa gaggctgctc aacggcgcgc gctcgatcag
     7981 ttactcgagg agtttcgtgt cgaggagagg cactatgtcc gggagagcaa gtctcttttc
     8041 ctgaaccgga agtcgatggt ggtgcaggag cggttgttct tccggagcat cccgctatcg
     8101 aattggatcg aacatgaaat ggtggtggaa gaaggagggg acctgcaaca actggcacaa
     8161 tcgtgctcgg tgttcgtgcc gcaagccgcg cgcgaagtgg ccgcagccgg tcccagactg
     8221 attcgcagcg cgggtgcgaa attgggggcg tgatagcatg agggtttggc cggaacattc
     8281 aggctctcac tatgcgaaac gtcgttatcg tcggatccgg ctgcgccgga aatactgctg
     8341 ctatttatac tgcccgcgcc aatctgaagc cgctggtcat tgcgggccat gagcctggcg
     8401 ggcaactctc gctcaccacg ctcgtcgaga attttccggg ttttcccgag ggaatcaacg
     8461 ggcccgatct tgtcgagaac atgaaaaaac aggcgcagaa tttcggcgct gagtacattc
     8521 acggctccgt gatcgaggca gatctttcaa agcggccctt ccggttgaac gtggagggtg
     8581 agtggctcga aactcgtacg ctgatcatcg cctcaggcgc ttcggctcgc tggctggggc
     8641 tcgaatccga gcagaagctg atcggccacg gcgtcagttc gtgcgctacc tgtgatgggt
     8701 tcttctatcg cgacaagcag attatagttg tgggcggcgg cgactcggcc atggaggagg
     8761 ctacgtttct cacgcgcttt ggacgtgagg tagcgctggt ccaccggcgt gacgaattcc
     8821 gtgcgtccaa aattatgctg gaccgcgcac gcgcgaatcc aaagatccgc ttcctcctta
     8881 acacggttgt agaagacgtc tatgacattg aaaaggggta cgttaccgcc gtaaaactcc
     8941 ggaatgtcca gaccggcgaa ctgtcggaac gggaagtgga tggtctgttt gtggccattg
     9001 gccatattcc gaacaccgct gcttttaaag gtcagatcga gctggacgag gacggctaca
     9061 ttaaatctcg cgggaatgcc cgcaccagtg ttacgggcgt ttttcatgcc ggagatgtag
     9121 aagaccgggt gtaccggcag gccattacgg cctccggggc cgggtgcatg gccgccatgg
     9181 aagcggaacg gtttttggag tctgaaggac actaaaattc cggttcctgg aacatttctc
     9241 ccgtcgtgcc gtattccttc atagggagca cgagcatgtt ctgcacacac tgcggtaccg
     9301 aacttgacgg aatctgccgg ttttgtcatc agtgcggcac cgctactacg aaacaggttc
     9361 cacgtccgcc ggccgcctca ccgggattaa gcattccgct ggaaggcagg aagatcggag
     9421 gcgtttgcgc aggctttgcc cgctacatcg gcgtggatgt tacgttcctt cgcatttttt
     9481 ggttgacgct cgccattttt actggagtgg gtttcgtcgc ctacctgatt gcctggctgc
     9541 tgatgcctaa ggagcgttac cttctacccg cgccatccac ggaaacggcc gggcagacgg
     9601 tgtagtcgcc ccccacttcc tcactatcct gagagaaata tccctccagg ttcactagag
     9661 cctatatgga gtgatgcctg cgcgtacaga gtctccgaag gctcgattag ccgttatcga
     9721 attccgggcg aagctgatcg gcgatcccgt caagcgtctg cgctttcttc gccgtgtggt
     9781 gaacacaagc gattttggcg atagtcccgc ttcgaactgg aggttgcgcg cgctcctcgt
     9841 ggcgattatc gtgattccgc tcctcatcga tacgagtccg gctgttatct ccgccgagtt
     9901 gcggacacca gccatatccc cactggtggg cagctacgat ccgttacccg tttggctggt
     9961 cgagcgcggc gcggagcatg aaacgtacag caacgggctt cgtatcgaaa atgctttccg
    10021 cgtcgccacg cacaaacgct cctaccagat tttcgaccgc gagtatccca accgcatcgc
    10081 cgcccgcaca aagacagacc cggcgggcat tgtcttccac accagcgaga gcacgcaatt
    10141 ggagttcgaa gaggagcgga acggtgcgtt acagcgcatt ggcgaatccc tgctcgggta
    10201 tgtccgcgcc aggcgctcct acaactttct gatcgaccgg tttgggcgca cattccgaat
    10261 cgttcccgaa agcgacgtcg ccaaccatgc gggacattcc atatgggcgg acgcgaagta
    10321 catttatctg aacttgaact ccagctttat aggagtttct ttcgaagcgg agacccaggt
    10381 gggagagcag ccgtctgtaa acccggcgca gattcacgcc gggcgcatgc tggccgctat
    10441 gctgcgttca caatatggga ttgcggttga aaactgtgtg acgcacgcgc aggtttccgt
    10501 gaaccccgcc aatatgcgaa tcggctatca caccgattgg gctgcgaatt ttcctttcgc
    10561 cgccatgggc ctgcccgaca attatgcgct cgcgcttccc agcatcgccg cattcgggtt
    10621 cacgtacgac gcaacattcc ttcgctccac cggccattcc ctgtggcgcg ggcttgccgc
    10681 cggtgaggaa ctgctgcgcc aatccgccgc tagcagggga gtgccggccg cggaatatcg
    10741 aaaagcactc caacgacagt atcaggaaag gatctcggcg ctccggagac tgggcgtcca
    10801 ggaggaaacc agcaatgaag caaactgaag gtaaggccat cccgatcggc ctcgatatcg
    10861 gcacaagccg catcgtattg gcccggcacg tggacaagga gtttcacttt gcgtcgcaac
    10921 tgaacgcatt cgtgcgaatt ccgttttcga aaatcaccga agacgttctt cgtaaggagc
    10981 agatccctta cgcagcagac ggttccgtgc tgtttgtgca cggcgatgca agccagcggt
    11041 ttgcggacct gctgaaatcc gagatcagcc gtccgatgga tcgcggattt ctgaatccca
    11101 gcgagccgga tggtttgcgg ctcatccgtg agatcgtcag ttcgatgacc ggcaaatccg
    11161 atacacgccg gaagttatac ttcaccgtcc ccgccgcgcc ggccggcgcc gaagagaatt
    11221 tcacgtacca tgaagctgcc ctccggcagg aactcgagga tctgggattc gaggcccgga
    11281 gcctgaacga gggccttgct gtcgtttatt cggaactgga agcgtcgaac ttcaccggaa
    11341 tcggagttag ttgcggaggt ggattgtgca atgtctgcct gtcgtatctg tcggtgccca
    11401 tcaccagttt cagccttccc aaagccggcg atttcattga tacgagtgcg gcctcggtga
    11461 cgggggaacg tgcgaaccgg attcggattc tcaaagagga atctttgtcc ctcaacaacg
    11521 gacatatgaa ggacaaggtg cagcgagtgc tcagtgtgta ctacgacgac atgatccggt
    11581 cgctcgcctc cgctctgaaa gagacgtttg caaatgtgcg tgggctcccg gaatttggca
    11641 agcccattcc actggtgctg gccggcggta cggcgatgcc caaaggcttc cgtgaccggt
    11701 ttgaagcggc cctccgcgaa accggcttcc ccattccgct ttccgagatc cggcttgcct
    11761 ccgatcctct gagcgcgacg gcaaagggag cactcgtagc ggcgctatcg gaaatgtgag
    11821 gttcagcctt tgggaggggg cacttgtccc tggtagttga tgatcgtgtc gacgatgcct
    11881 aattcgacgc ggcggttctt ggcccgggcg ttcgagttgg tggctttcac ggctggactg
    11941 gattttccaa atccctgtgt ggtgataatc tcggacggaa ttccagcctg caccagatag
    12001 tcgcggatgg tctgcgctct gcgctcggac aaattctgat tgtattcccg cgtcccgaca
    12061 tcgtctgtgt gtccgtacac gaacaggcgg tacccttcgg acgccagcag cacacccgcg
    12121 atgcggctga ggacctcgcg gttttccggc cgcagactgg ctttatcgaa atcgaagtgg
    12181 aatgagtcgt cggcaagctc catcaccatg ccggccggag tgcggcgcgt gggagcaatg
    12241 cggttgagcg cctcctgcat gcggttcaat tcgtcttccc gccgcttgcg aatggtctcc
    12301 agctcggagc gcgtttgctg ctccgccgcc gcggcagcct cggcgcgctg gtgtgcagtc
    12361 gttgcctgtt gctgcgcttc ctgcgccgcc tgctgtgcct gctcagcctg caattgagca
    12421 gccgtcctgt cctgttcggc cgcactggcc gcagactctg cctcagcctc acgctttttg
    12481 gccgcctcgg ctcgctgtat ggcgtctgtc acttcgtccg agtaacgggc caactgcttt
    12541 tccacattgc cggctcgcgt ttcggcctct tcgagttgcc ggccgagttg gttgaggcgg
    12601 ctggtggcga gccaaacggc ggtcccaatg gctgcaatca ccaccaccac aaggccgacg
    12661 gtcaaacggc gtgaatggcg acggtctgcc atgctactac gcccattgtc gcatggcccg
    12721 gcgccgctca tgaaacggcg ttaacgaagt ccgccggggc gtgcggacgc gagtccaggc
    12781 agtccaccca gaccgggcga taatcgcacg gcgcgttgta cagactcggc gacagcttcc
    12841 acggcagcca ctcccagcgc gttcacatcc gcatcacgcg tgccggtgga aagggcgaca
    12901 acgaggtcac cgtcgtagga ggtccaaacc ggggaaatgg tccgtgctat ccccacgctt
    12961 gccaactgtg ccaggcgagt ggcctgcacc ttgctcagcc gcgcgttcgt cgcaaccacg
    13021 gccagggtcg tgttttcgtg gcgcgcagga gcctgccgca ctgccttcat cgccgcggcg
    13081 gttcccacca gttccaggct ctcttcggat gctctggctc ccgccagcag tttgcctgtc
    13141 tgcgggtcga tgacatcgcc ataagcgttc actaccgtca gagaggagac gagaacgtcc
    13201 gtgtggctgc ctcccaatgc gacagtgaag gtcccgagcc cggatttcat ggcgcgcgcg
    13261 attccgaaaa tcttgcccgt ggtggctccc gtgccggcgc cgacggcgcc ctcctcaacc
    13321 actcccgtgt gcgccgcggc tgccgccgct tcccccatct cgcgagtcgg ccgcacgccg
    13381 gcttttccaa ttgtcagatc gtagaggatg gcgcagggga ctagaggtac cttcgcggcg
    13441 cgtgtcgtgt acccgatgcc cttctgctcc aggtagcggc gcaccccgga ggccgcttcc
    13501 agcccgaaag cgctgccgcc ggcaaacacc agcgcatgta tgtgatcggt gacgtgcagc
    13561 gggctcatca cttcgaactc ttcggttccg gtcgctccac cccggatgtc gtatccggcg
    13621 acggcgccgt cttcacacag aatgacggta cacccggtga gcgcatcgta atcggagctg
    13681 tggcctaccc gaatccccgg aatatcggtt aagcctttca tttcagttcg tatggtagca
    13741 tcgaagttac gagggtcatt tcggtttgct cgacatcctg aaaccgcctg tccttgcgct
    13801 ccaggatttc tattcgctcg cgggcagggc aatccggaac atcgttcgcc gcccacatta
    13861 ttacgacgac atcgttctcc agatggacgt catcggagta ggcagcctgc cgatcgtgct
    13921 gctgaccggc ttcttcagcg gggccgtgat ggccctccag atgtcacgcg cgctttcgca
    13981 gtacggcgcc acgggacagg taggcatgat cgtatccatt actcttgtgc gcgaactggg
    14041 tccggtgctt accgcggtgg tggtcgccgg gcgcaacgcc tcgggaatgg cgagcgagct
    14101 cggatccatg aaagtcaccg agcagatcga tgccatgcgc gcgctgggta cggatccggt
    14161 ccagaagctc gttacgccga ggctgattgc gacggccgtg gtcctgcccc tgctgacgat
    14221 cgtcgcggat tttgtcggcc tggttggagg ctatgtcgta gccgttccat tgctgagcct
    14281 gaccacagca ggtgaatact ggaacacggc ctggcgcgcc atcgaataca acgacattgc
    14341 acaagggttg ttgaagcccc tggtgttcgc catcattatc gcccttgtcg gttgttttta
    14401 cggcatgcgg accactggag gaacgcaagg cgtcggacgg gcgacc
//
LOCUS       DASTMK010000605         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_414812, whole genome shotgun sequence.
ACCESSION   DASTMK010000605 DASTMK010000000
VERSION     DASTMK010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414812"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFQ79_24980"
     CDS             complement(<1..756)
                     /locus_tag="VFQ79_24980"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0142999.1"
                     /translation="MKTQAQSLSVKRAEVEFHSFASLGEPERAIAAYAEENVRRSGII
                     RNHLSMIGSMSPFLEIGSNVGHTSYMLCNEFGAGGFALDISADALRYGSVLQERWNLS
                     RVPIRIAGDAVDLPFRDGSIRFVMGFQVLSQFLDIESVFLEVKRVLAPGGVFFFSEEP
                     LKRLLSLRLYRCPYYETMKPWERKLSDWGLLGYLVRDVIGAHQEESFGIRQNHTMRLQ
                     DWHALITRHFVEKEYELFVPERGWGERVVKRAAI"
     gene            complement(753..1505)
                     /locus_tag="VFQ79_24985"
     CDS             complement(753..1505)
                     /locus_tag="VFQ79_24985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0143000.1"
                     /translation="MKPALLTISCLLVLACGTPEKTSTIAIVGAVLIDGTGAAPVDDS
                     VVLIAGSRIRAVGTRTATPISAAYQKVDARGLYLLPALYRVPAEQLPYVSTEKELLQV
                     VDAGARAVAGMVTDKDVTGTELADHLRRLDVVMVPALSRIQGSLAALNRAKHNTAALA
                     RSGVRMGLAAGGAEQLELELLVESGMPSSEIIRAATSNGALAAGRATEAGTIEAGKYA
                     DLVLLSANPLDDARNLRKVEKKMVRGEWTLAK"
     gene            complement(1502..2206)
                     /locus_tag="VFQ79_24990"
     CDS             complement(1502..2206)
                     /locus_tag="VFQ79_24990"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0143001.1"
                     /translation="MASDSRQAAPAVVLFDIDGTLIRRAGPHHRQALVEAVRTVTGLD
                     TSTDHIPVQGMLDRDILTRMMTDAGATRRMITASMPAVVREAQRIYVRNCPSLETKVC
                     PGSRELLQQLLDRGIPVGLVTGNLTRIAWKKMQRARLRRFFRFGAFAELGRSRGELVR
                     IAVRYARRHGWISPQTLISLVGDHENDIRAAQANGIRSIAVATGLSPMAGLATASPDV
                     LVPDLRMLPLASLLEK"
     gene            2284..2886
                     /gene="cyaB"
                     /locus_tag="VFQ79_24995"
     CDS             2284..2886
                     /gene="cyaB"
                     /locus_tag="VFQ79_24995"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEU0143002.1"
                     /translation="MALSANHEIEIKLRIPSAEEGRNLLPRAGFQVVADRVFETNMVF
                     DFPDQRFRRERKLLRLRCAGGRSVLTYKGTPEEGPFKSREEIELEASDPRQAELLFEQ
                     LGLQQVFVYEKYRTEYRRAGDGAIVMLDETPIGDFLEIEGTPEEITSAAVQLGFGPDQ
                     YINSSYGTLYRDWCRELNLEPSNMTFKTRPDAPPEKNTQA"
BASE COUNT          649 a          952 c          963 g          644 t
ORIGIN      
        1 tatggccgcg cgtttcacca cgcgttcacc ccagccccgt tcgggcacaa acaactcgta
       61 ctctttttcg acgaaatgcc tggttatcag ggcgtgccag tcctgcaaac gcatggtgtg
      121 gttctggcgg ataccgaagc tctcctcctg gtgggcgccg attacgtcgc gaaccaggta
      181 acccagcagt ccccaatcgg acagcttcct ttcccagggc ttcatcgtct cgtagtaggg
      241 gcatcggtag agccgcagag acagcagccg tttcagcggt tcttcggaaa aaaagaaaac
      301 accgcccggc gcaagaaccc gtttcacttc taggaatacg ctctcgatat cgagaaactg
      361 gctcagcacc tggaacccca tgacaaaccg gatagagccg tcccggaagg gtaggtcgac
      421 ggcgtcgccg gcgatgcgta tgggcactcg tgagagattc caccgctcct gcaggacgga
      481 accatatcgc agcgcgtcgg cagagatatc gagcgcaaaa ccgccggcgc cgaactcgtt
      541 gcagagcatg tagctggtgt gaccgacgtt cgatccgatc tcgaggaacg gcgacatgct
      601 tccgatcatg gaaagatgat tgcgaatgat accggagcgc cggacattct cctcggcgta
      661 agcggcgatg gcccgttccg gctcgccgag cgacgcgaag ctatggaatt ccacttccgc
      721 gcgctttacg ctgagggact gcgcctgcgt tttcatttag ccagggtcca ttccccgcga
      781 accattttct tttctacttt gcggagatta cgcgcatcgt ccagcgggtt ggcggaaaga
      841 agaaccaggt cggcgtactt accggcctcg atggttccgg cttcggtggc acggccggcc
      901 gccagcgcgc cattgctggt ggcagcccga atgatttccg aagacggcat tcccgattct
      961 accagtaact ccagttcgag ttgttcggcg ccgccggccg ccagacccat ccggacgccg
     1021 ctcctggcaa gcgcggcggt attgtgcttg gccctgttga gggctgcgag cgacccctga
     1081 attcgcgaga gtgccggcac cataacgaca tccaggcggc gcaggtggtc tgccagttcc
     1141 gtgccggtga cgtccttgtc ggtaaccatg cccgctacgg ctcgcgcgcc cgcatccacc
     1201 acctgtagaa gttctttttc cgttgaaacg tatggtagtt gctcggcggg taccctatac
     1261 agggccggca gcagatagag gccgcgcgcg tccacctttt gataggcggc cgagatgggc
     1321 gtcgcggttc gcgtgcccac cgctcgaatg cgggatcccg cgatgagaac gacggaatcg
     1381 tccaccggag cggcaccggt tccgtcgatc aacacggcgc cgacaatcgc aatggtgctg
     1441 gttttttccg gggtgccgca cgcgaggacg agcaggcagg agatcgtcag aagtgcaggt
     1501 ttcatttctc gagcagactg gccaggggaa gcatacggag atcgggcacc agaacatccg
     1561 gggatgccgt ggcgaggccg gccatagggc tcaatccggt cgcgacggcg atcgatcgga
     1621 ttccgttcgc ctgcgccgcc cggatgtcgt tctcatggtc tcccaccaga gaaatcaggg
     1681 tttgtggcga gatccaaccg tgcctgcgtg cataccggac ggcgatgcgc acgagttctc
     1741 cgcggctgcg gccaagctcg gcgaaagcgc cgaagcgaaa aaatctccgc aggcgggccc
     1801 gctgcatctt cttccaggca attcgtgtca ggtttccggt gaccaggccc accggaatgc
     1861 cacggtccag caactgctgc aacagctctc tcgatcctgg gcagaccttc gtttcgaggc
     1921 tgggacaatt gcgaacgtat attcgctgcg cctcccgcac gacagccggc atgcttgcgg
     1981 tgatcatccg ccgggtggcg ccggcgtccg tcatcatgcg cgtcagaatg tcccggtcga
     2041 gcataccctg cacgggaata tgatctgtgc tggtatccaa accggtgaca gtccgcacgg
     2101 cttccacgag cgcctggcga tgatgcggac ccgcacgccg gatcagtgtt ccatcgatat
     2161 cgaacaacac aacggcggga gccgcctgcc tggaatcgga cgccatttac atccaatata
     2221 gccgctatcg ccggagagcc tcgtccgcgg gctgtggcag gttgctacca tcggcactga
     2281 agcgtggctc tttcagcgaa tcacgaaatc gagatcaaat tgcggattcc gagtgcggag
     2341 gagggtcgaa atctgctccc gagggccggg ttccaggtcg tagctgaccg ggttttcgag
     2401 accaatatgg ttttcgactt ccccgaccaa cgcttccgca gggaacgcaa actgctccgg
     2461 ttgcgctgcg ccggtggccg ctctgttctc acctacaagg gaacgccgga agagggcccg
     2521 ttcaaatcgc gggaagagat cgagctggag gcaagcgatc cccggcaggc cgagctcctc
     2581 ttcgaacagc tggggcttca gcaggtgttt gtatacgaaa aataccggac cgaatatcga
     2641 cgtgcgggcg acggcgccat tgtgatgctg gatgagactc cgattggcga ctttcttgaa
     2701 atcgagggaa cgccggagga gattacatcc gccgccgtac aattgggctt cggtccggat
     2761 caatacatca actccagcta cggtacgctg taccgggatt ggtgcagaga gctgaatttg
     2821 gagccctcga atatgacctt caagactcgc ccggatgctc caccggagaa gaatactcag
     2881 gcatagtact gagcttttca accaacttgc cttagacgct cgaaaagcct tgttcgtggg
     2941 aaggcccaat ggtattctca agctgtcctg gctcagtacc atgagtaccg gggcaaggtc
     3001 tcgcgtatga tcacgagcat tctgataagc gtattgtcaa tattgctgct tgcgtattgg
     3061 tttcgataca cgtgtcaatt gatcctgggg gcgcggacga gcaaagatta cgcccaacag
     3121 gtcgccatcg cgaatcagct caattttgtc gatgtccaag cccgattgcg ggaggatggc
     3181 tcggcccggc ttgatcctct gtggcagt
//
LOCUS       DASTMK010000606         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_511714, whole genome shotgun sequence.
ACCESSION   DASTMK010000606 DASTMK010000000
VERSION     DASTMK010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511714"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFQ79_25000"
     CDS             complement(<1..461)
                     /locus_tag="VFQ79_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0143003.1"
                     /translation="MNRRGFHFAAAVVLLSLWIWSPPPDGVEVAYVGPGAGFAFLGSL
                     LALVAGFLLSLASLLLWPFRVLRASMRRRRAFRHARVKKLVFLGLDGLDYRLTERYLK
                     EGKLPNLARLREQGSFSALRTTFPSLSPVAWSTFATGVSPAKHNIFDFLNR"
     gene            complement(458..1324)
                     /locus_tag="VFQ79_25005"
     CDS             complement(458..1324)
                     /locus_tag="VFQ79_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0143004.1"
                     /translation="MVSIPNPPPRGNNYILVVLDSCRYDSFIAARPRTMRKLGKLERR
                     WSYASWTAPSHYNLLMGLMPHSSPKHVFASEYYKRDFVRFQDRLGQMNFEFKSLIPAL
                     YLPTFLRNSLGYQTNAFVSLPVLNPKTPLNSGFDRYRLMEKHNDMRAMLREMRFSEER
                     PSFYLLNVGETHYPYALPDEPPDEWPRISGVHGVFKHLDEHVTGGKLPKRRSPFFDQA
                     KLDTLRARQIAAVKYLDTVFEELFDLVPRNTYITITSDHGELFGEDGYFGHGPIQHEK
                     VLEVFLLEGKLR"
     gene            complement(1349..>2494)
                     /locus_tag="VFQ79_25010"
     CDS             complement(1349..>2494)
                     /locus_tag="VFQ79_25010"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0143005.1"
                     /translation="EAEGRRFLLREGEWSEWVRVSLPLLPPFQNAHTMFCVYAKQLRP
                     ALKIYVSPLNIDPFSPDLPISTPPSYSAELAAAVGPYYTQGIAEDTAAVREGVLTLDE
                     YGQQAGLVEREQFALLHHVLRTTAHGVVFFHFLGIDQDSHILWGRHEAALLETYKKTD
                     VEIGRVARTYPNDTLIVMSDHGFSEFRRAVHLNRWLMLEGFLTLDLPFNTGDEEGLVH
                     VDWAATQAYSIGLNGIYLNLAGRERNGIVKPSERDALVDRIVHRLKALRDPETGETVV
                     AEAYRARDVYRGEASASAPDIVVGWNTGYRSSWQTALGAVPSVIFEDNKDEWRGDHCI
                     AAHLVPGVFLSNRKTRRRELRLEDITVTLLNEYGVKPGAGMTGRPVF"
BASE COUNT          521 a          765 c          755 g          453 t
ORIGIN      
        1 ctccggttga ggaaatcgaa aatgttgtgc ttagccgggc tgactcctgt agcgaacgtc
       61 gaccaggcca cgggagagag cgagggaaag gttgtacgta gagcggagaa cgacccctgc
      121 tcccgcagcc gggccaggtt cggcagtttc ccttctttga ggtaacgctc cgtgagacgg
      181 tagtcgaggc catccaaccc gaggaagacc agcttcttca cgcgggcatg acgaaacgcg
      241 cgccgccttc gcatggacgc gcgcaacacc cggaacggcc acagcagcag cgaggccagg
      301 ctcagcagga agccggcaac cagggcaaga agcgagccga gaaaagcgaa tccggcgccc
      361 ggccccacgt acgcgacctc caccccgtcc ggcggcggtg accagatcca cagcgacaga
      421 aggactaccg ccgccgcgaa atggaaaccg cggcggttca tcggagcttg ccttccagca
      481 gaaacacctc gaggaccttt tcatgttgta tcgggccatg gccgaaatag ccatcctccc
      541 cgaacaactc gccgtggtcg gaagtgatgg tgatgtaggt gttccggggc accaggtcga
      601 acagctcctc gaacacggta tcgagatatt tcaccgccgc gatctggcgt gcccgcagcg
      661 tatccagctt cgcctgatcg aagaacggag atctgcgctt cggcagctta cctcccgtga
      721 catgctcatc aaggtgcttg aacacgccgt gcacgccgct gattcggggc cactcatcgg
      781 gcggttcgtc gggcagcgcg taggggtagt gcgtctcgcc cacattgagc aggtagaaag
      841 acgggcgctc ttcggaaaag cgcatctccc gcaacatggc acgcatgtcg ttgtgcttct
      901 ccatcagccg gtagcggtcg aagccgctat tgagcggagt cttgggattc agcaccggca
      961 gcgacacgaa ggcatttgtc tggtaaccca gcgaatttct caaaaacgtt ggcagataca
     1021 acgccgggat cagcgatttg aactcgaaat tcatctgccc caggcgatcc tggaatcgca
     1081 cgaaatcgcg tttgtaatac tcggatgcga acacgtgctt cgggctcgaa tgcggcatca
     1141 gccccatcaa caggttgtag tgcgacggcg ccgtccaact cgcgtaagac catcgccgtt
     1201 ccagcttgcc gagtttgcgc atggtccgcg ggcgcgcggc gataaaggaa tcgtagcggc
     1261 agctatcgag gaccaccagg atgtagttgt tcccccgcgg cggaggattg ggaatagaga
     1321 ccacgttgtc ccgattatac ctacgcgttc agaacaccgg gcgtcccgtc atcccggcgc
     1381 cgggtttcac gccatactcg ttcagcagcg tgacggtgat atcctccaac cggagttccc
     1441 ggcgccttgt cttgcggttc gacagaaaga cacccgggac cagatgggcc gcgatgcagt
     1501 ggtcgcctcg ccactcgtcc ttgttgtctt caaaaatcac ggaaggaacc gcacccaaag
     1561 ccgtctgcca ggacgaacgg tatccggtgt tccagccgac tacgatgtcc ggagcggaag
     1621 cgctcgcttc gccgcgataa acatcgcgcg cccggtacgc ctccgcaacc acggtctcgc
     1681 ccgtctcggg atctcgcagc gctttcagcc gatgaacgat ccggtcgacg agggcgtcac
     1741 gttcggaagg tttaacgatt ccgttgcgct cccgccctgc cagattgagg taaatcccgt
     1801 tcaggccgat ggaataggct tgcgtggccg cccagtccac atggaccagc ccctcttcgt
     1861 cgccggtgtt aaacggcaga tccaatgtca gaaaaccttc gagcatcagc caccggttca
     1921 gatggacggc gcggcggaac tcggaaaatc catggtcgga catcacgata agagtgtcgt
     1981 tagggtaggt ccgcgccacg cgcccgatct cgacgtcggt cttcttatac gtttcgagca
     2041 gcgcggcctc atgccggccc cagaggatgt gggaatcctg gtcgatgccc agaaaatgga
     2101 agaagaccac tccatgcgcg gtggtgcgca gcacatggtg tagcagggcg aactgttccc
     2161 gctccaccag gcccgcttgt tggccgtact cgtcgagcgt caacacgcct tcccgtaccg
     2221 cggcggtgtc ttccgcgatg ccctgcgtgt agtagggtcc caccgcggcg gcaagctccg
     2281 cgctgtagga aggcggtgtc gagatcggaa ggtccggcga gaacgggtcg atattcagcg
     2341 gtgaaacgta gatcttcagc gccggccgga gctgttttgc gtacacgcag aacatggtat
     2401 gtgcgttctg aaaaggaggc agcaacggaa gtgagacgcg aacccactcc gaccactcgc
     2461 cttcgcgcag gaggaagcgc cggccttctg cttc
//
LOCUS       DASTMK010000607         7659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_350656, whole genome shotgun sequence.
ACCESSION   DASTMK010000607 DASTMK010000000
VERSION     DASTMK010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350656"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..2751
                     /gene="pta"
                     /locus_tag="VFQ79_25015"
     CDS             445..2751
                     /gene="pta"
                     /locus_tag="VFQ79_25015"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU0143006.1"
                     /translation="MSIRNEDALEYHSSAPAGKIAVHPTKPCRTQRDLSLAYTPGVAV
                     PCLEIQRDPSLAYKYTAKGNLVAVVTNGTAVLGLGNIGPAAGKPVMEGKGVLFKRFAD
                     IDVFDIELNTSDPKEIIRVCQLLEPTFGGINLEDIKAPECFEIEEELRRTMQIPVFHD
                     DQHGTAIISGAALLNALELAGKRIDEVKVVFNGAGASGLACAEHYMRLGISRENIVMC
                     DTNGVVYKGRTKGMNPYKERFARETTLRTLAEAMEGADVFFGLSAANCVTPEMLLSMA
                     PDPIVFALANPDPEIAYDIAIATRKDLIMATGRSDFPNQVNNVLGFPFIFRGALDVRA
                     TAINDEMKLAATRALASLAREDVPDAVRRAYGVESMHFGRNYIIPTPFDSRVLIWVAS
                     AVAQAAMDSGVAQSPIDIGEYKDQLERRLGKAVEVMRVMIHKAQRHPQRVVFPEGQEA
                     KILRASQILLDEKIATPVLIGREDKIRQQIESLRLHLQDVAIVDPRTFPRMSVYSEEL
                     YRLRQRKGVTRNEAAVLIQNRTAFGAMMLHMGDADALIAGLTQHYPDTIRPALQVIPP
                     REGLKRVAGVYMMITPQGKIYFFADATVNIEPSSEDLAEIALEAAYMAKSFDVEPRVA
                     LLSFSNFGSTRHPLSERVRRAVDIVKQRAPEIMVDGEMQADTAVVPEIIDQTYPFSTL
                     KGGANVLIFPSLEAGNIAYKLLMRIGGAEAIGPILMGLSKPVHVLQRGAEVNDIVNMA
                     AIAVVDAQAAAGLGGSEIDPRASASGIG"
     gene            complement(2753..4408)
                     /locus_tag="VFQ79_25020"
     CDS             complement(2753..4408)
                     /locus_tag="VFQ79_25020"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0143007.1"
                     /translation="MRGTFIIVLLSMLGTVPGQVASQEQKCSVEGRVTAAATGEPLRR
                     ATVILRTTEGRENPYSASTDPSGRYVIPGVEPGRYRLYAQRDGFVRQEYGQKTTERSG
                     VPVVLSAGQDLKNADFQLIRSGVISGRVMNEDGEPAPLVRIQGYRRAYVQGRRQLMPV
                     GMALTNDLGEYRLFGLQPGSYYVSAVSSRNTAGMVFGRGAAARGRMAAPAESGYPPLF
                     YPNALDARQAAQIQVTPGGEARGTDFRLLPTSTVRLRGRIDGLPAEMRRQAMITLVPR
                     EELVVNFANRTNVRADPQGNFEIRGVVPGDYTLAAEVFQQGKRQSARRVIQVGTADLD
                     DIVLTLASPVSLSGYIRTEGEGTVDLSQIRVYLQPLEPSRLGGAGTGEVRADGLFVVE
                     NVVPGRYQVYVIHRSDEFYLKSVRMGNQEIAGGELDLSSGAAPAPLELVLRAGAGQVS
                     GMVMGPENSPALGVTVVAVPDESRRNESRLYKSVASDQSGQFTLKGLVPGEYKLFAWE
                     EIEPGAYQDPEFLKPFESRGKAITVAENSRESVQLQMIPAGTR"
     gene            4466..5638
                     /locus_tag="VFQ79_25025"
     CDS             4466..5638
                     /locus_tag="VFQ79_25025"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0143008.1"
                     /translation="MLAEVSDLHEFIRDRLAPHFAELARCDSTKELVAHPLYRKLLPS
                     IETVLSTLDTGDFRKHTAEAQSSYRFVAWNLERGTEVEGQIEALRCDSYLSGADVLLL
                     TELDIGMARSANRDIPRLLARELGMQYAFAPCYLNLTKGAGAECEAQGENQLGLHGNA
                     ILSRYPIENARAVLFPNGRDKMRGREKRLGSQTAVLAEIWFPGSSVTAAAVHLDAQST
                     QRHRYEQMRLLLDAVPPEGPAIIGGDWNTSTYNSSRALHAIIGFAVRVLMGVGNVIRN
                     HYLHPYHRFERDLFQLLENRGFDYRSSNLLGVRTAGFCIADERANKNLGEWVPAWCFH
                     FIRWALRNHGGKCPLKLDWFAVRGLRCKNPTVLHAFREERATPLSDHDPIGIDVVV"
     gene            complement(5641..6621)
                     /gene="galE"
                     /locus_tag="VFQ79_25030"
     CDS             complement(5641..6621)
                     /gene="galE"
                     /locus_tag="VFQ79_25030"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU0143009.1"
                     /translation="MSRILVTGGAGYIGSHTAHVLAEHGFDVVIADDLSRGHRHNVAP
                     ERLHVVRMQDTDALTALMIAGRVEAVIHFAAYIAVGESTREPELYFNNNLSGSLSLLT
                     AMQRAGVKRLVFSSTAAVYGNPTSVPISEDAPYAPVSPYGESKVMVEKVLRWLSELRG
                     LKSVCLRYFNACGADPAAGLGEEHDPETHLIPLLLRAIGTGTPITIFGDDYDTPDGTC
                     IRDYIHVRDLAEAHVLALESLLSGGESDVFNVGTGSGYSVMEVLRTVEKIVGKRVPYT
                     VGPRREGDPPVLVANPDRLQRSLGWSPRSSSLETVVRSAWEFEQNRAVAG"
     gene            6668..>7659
                     /gene="purM"
                     /locus_tag="VFQ79_25035"
     CDS             6668..>7659
                     /gene="purM"
                     /locus_tag="VFQ79_25035"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU0143010.1"
                     /translation="MPKKQVRYRDAGVNIDEADRAVSRIKKLARATFTKGVLTDIGSF
                     GAGFQLDGWKKPVLVSSADGVGTKLKVAFLTGQHDTVGEDLVNHCVNDILVQGARPLF
                     FLDYFAIGKLDAEVAEQVVTGISRGCRLNGCALIGGETAEMPGLYAPGEYDLAGFIVG
                     AVERRALITGGNIKPGDVLIGLPSNGLHTNGYSLARKLLFDVAGYGPKTFLPELQSSV
                     GEELLKVHRSYRKPMLALLEAGLLKGAAHVTGGGISDNLPRVLPKGLAARINAASWPV
                     LPIFEVLKRVGNIPEEDYRRTFNLGLGMILAIGSRKLRAAVRLLDALKEPYHQIG"
BASE COUNT         1538 a         2310 c         2305 g         1506 t
ORIGIN      
        1 gccggcggtg gtcttcacca atgggtaaat ggtcatttgc ttgatttccc tttgttgctc
       61 cgatatgccc gggatttccc gggcaggtaa ctctgcccac actccgccca tacgaaacaa
      121 acggagtttg tcagacaggc gggcggttgc tcaggcttgt tcagccgcac cggatggttc
      181 cggcgccatc gcgcttctga tgatacccgc acgacacgcc gggtcaggcc aaatgcagag
      241 ttgtcaaggg atgttttgac ttcggcaata aggaatataa gtaattgaaa ctaagccgct
      301 tactctaaca ccgattggca gggatgggtc tgttcggaag tctcgtgaac ggcatagagc
      361 gaaaggcggt aatcccatcc ggtagggcgg atgcgtcccc ttcgcaaaaa gagtgagaat
      421 caagtggata gaaaggattg tttaatgagc attcgtaacg aggatgcact cgaataccat
      481 tcctccgccc cggccgggaa gatcgccgtt catcctacta aaccctgccg cactcagcgc
      541 gatttgagtc ttgcctacac accgggcgtt gccgttccct gcctcgagat ccagcgggat
      601 ccgtcccttg cctataaata caccgcgaag ggcaatctgg tggctgtggt caccaacggc
      661 accgctgtcc tcgggctggg aaacatcggt ccggccgccg gcaagcctgt gatggaaggc
      721 aagggcgttc tcttcaagcg cttcgcggat atcgatgtct tcgatatcga gctgaatacg
      781 agcgatccga aagagatcat ccgcgtctgc cagcttctgg agcccacttt cggcggcatc
      841 aatctggaag acatcaaagc gccggagtgt ttcgaaatcg aagaagagct acggcgcacg
      901 atgcagatac cggtcttcca cgacgatcag cacggcactg ccattatttc gggcgccgcc
      961 ctgctgaacg cgctggaact cgcgggcaaa cgcatcgacg aggtaaaggt tgtattcaat
     1021 ggcgccggtg caagcggatt ggcctgtgcg gaacattaca tgcggctcgg aatcagccgc
     1081 gaaaatatcg tcatgtgcga tacgaacggg gtggtttata aaggccgtac caagggcatg
     1141 aacccgtaca aggaacggtt cgcccgggaa accacgctgc gaactctcgc ggaggccatg
     1201 gaaggcgcgg atgtgttttt cggcctctcc gcggcgaact gcgtcacacc tgagatgctg
     1261 ctctccatgg cgccggaccc gatcgttttc gccctggcga atcccgatcc ggagatcgcc
     1321 tacgatatcg ccatcgccac ccggaaggac ttgattatgg cgacggggcg gtcggatttt
     1381 ccgaaccagg taaacaacgt cctcggcttt ccgttcatct tccgcggggc cttggacgtg
     1441 cgtgccacgg cgatcaatga cgaaatgaaa ctcgcggcca cacgagcgct ggcctcgctt
     1501 gcaagggaag acgttcccga cgccgtacgc cgcgcctacg gcgtggaaag catgcacttc
     1561 ggacgtaatt acattattcc gacgccattt gattcccggg tgctcatctg ggtggcctcc
     1621 gccgtcgcgc aggccgccat ggatagcggg gtagcgcagt cgcctatcga tatcggggaa
     1681 tacaaggatc aactggaacg gcggctcgga aaagcggtgg aggtgatgcg cgtgatgatt
     1741 cacaaagcgc agcggcatcc ccagcgcgta gtgtttccgg aggggcagga ggcaaaaatc
     1801 ctgcgcgctt cccagatcct tctggatgag aaaatcgcta caccggttct gatcgggcgc
     1861 gaggataaga tccgccagca aatcgaatcg ctgcgacttc acctccagga cgtcgccatt
     1921 gtcgatccgc gaacattccc gcgcatgagc gtctacagtg aagagcttta ccggctgcgc
     1981 cagcgcaaag gagtgacgag aaacgaagcc gctgtcctga ttcagaacag gacggcgttc
     2041 ggggccatga tgctgcacat gggcgatgcg gacgcgctga tcgcggggct gacgcagcac
     2101 tacccggata ccatccggcc cgcgttgcag gtgattcccc cgcgcgaagg gctgaaacgt
     2161 gttgccggtg tgtacatgat gattaccccg cagggcaaaa tctacttctt cgccgatgcc
     2221 acggtgaaca tagagccgtc ttcggaagac ctcgcggaga ttgctctcga agccgcttac
     2281 atggcgaaaa gtttcgatgt cgagcctcgt gtggcgctac tttcgttttc caatttcggc
     2341 agcacgcgcc atccgttgtc cgagagggtc cggcgggccg tcgacatcgt aaaacaacgc
     2401 gctccggaga tcatggttga cggcgagatg caggccgata ccgccgtggt cccggaaatc
     2461 atcgaccaga cttatccgtt cagtactctc aagggcggcg caaacgtttt gatctttccc
     2521 agtctggaag cgggaaacat cgcctataag ctgttgatgc ggattggcgg cgccgaggcg
     2581 atcgggccga ttctgatggg cctctccaaa ccggtacacg tgcttcagcg aggcgccgag
     2641 gtgaacgaca ttgtcaatat ggcagccatc gcggttgtgg acgcgcaagc cgccgcgggg
     2701 ctgggcggct cagaaatcga tccgcgagcg tcagcaagcg ggatcggata ggtcaccggg
     2761 tgccggccgg tatcatctgc aattgaacgg attcccggct gttctccgct acggtaatgg
     2821 cttttccgcg ggactcgaac ggtttcagaa attccggatc ctgatacgcg ccgggttcaa
     2881 tctcctccca ggcgaataac ttgtattcgc ctggaacgag tcccttgagg gtgaactggc
     2941 cgctttgatc cgaggcaacc gacttgtaca ggcgcgattc gttgcggcgg gattcatccg
     3001 gaaccgcgac cacggttacg cccagggcag ggctgttttc cggacccatt accatgccgg
     3061 acacctggcc cgcgccggcg cgcagcacca gctccagcgg ggcgggagcg gcacccgagc
     3121 taagatcgag ttctccgccc gctatttcct ggttccccat ccgcaccgac ttcagataga
     3181 actcgtccga acggtggatg acatacacct gataccggcc gggaacgacg ttctccacga
     3241 caaacaagcc atcggctctt acttcgcccg tgcctgcacc tcccaggcga gagggctcga
     3301 gaggttggag atacacgcga atctggctca ggtcgacggt gccttccccc tccgtgcgga
     3361 tgtacccgct aagggacacc ggcgaggcca gcgtcaggac aatatcatcc aggtcggcgg
     3421 taccgacttg gatgacacgc ctcgcgctct gccgtttgcc ctgctggaag acttccgccg
     3481 ccaacgtata atcgcccggg actacgccgc gaatctcgaa attgccttga ggatcggccc
     3541 tgacgttcgt ccggtttgcg aagtttacga caagctcctc gcgcggcacc agggtgatca
     3601 tcgcctgtct cctcatctcg gcgggaagcc cgtcgatccg cccgcgcagc cttaccgtgc
     3661 tggttggaag taaacgaaaa tcggtgccgc gtgcttcacc gcccggggtt acctggattt
     3721 gggctgcctg gcgggcgtcg agagcattgg gataaaacag cggagggtag ccgctttccg
     3781 ccggggcagc catacggccg cgcgccgccg cgccccgtcc gaaaaccatg cctgccgtat
     3841 tccgggagct gacggcgctc acgtagtagc ttccgggctg gaggccgaaa agccggtatt
     3901 ctcctaaatc gttggtgagc gccattccca caggcatgag ctgccgcctg ccctgtacat
     3961 atgcacggcg atagccctga atgcggacga ggggtgcggg ttcgccgtct tcgttcatca
     4021 cgcgtccgct gataacaccc gaacggatca gttggaaatc ggcgttcttc aaatcctggc
     4081 ccgcgctcaa cacgaccggg acgccggacc gttccgtggt cttctggccg tactcctgcc
     4141 gcacgaatcc atcgcgttgc gcatacaacc ggtatcgccc gggctcgacg cccggaatga
     4201 cgtaccgccc ggatgggtcg gtgctggcgg agtaagggtt ctcccggcct tccgttgtcc
     4261 gcagaatcac tgtggcccgt cgcaggggct cgcctgtggc ggcagccgtc acccgtccct
     4321 ctacggagca cttctgctcc tggctggcga cttggcccgg caccgtcccg agcatagaca
     4381 acaatacgat aatgaaagtt ccccgcattt ggctcccgaa atagattaga cgccgcttga
     4441 gcccgcgggg ttatgacaca ctgcgatgct tgccgaagta agcgaccttc atgaattcat
     4501 ccgggaccgg cttgcgcctc atttcgcgga actggccagg tgtgattcca cgaaggaact
     4561 cgttgcccat ccgctctacc gcaagctgct gccatccatc gaaaccgtac tttccactct
     4621 cgacaccggt gatttccgga agcatacggc agaggcacaa tcttcctacc ggtttgtcgc
     4681 ctggaacctg gagcggggaa cggaagtgga agggcagatc gaagcgctcc ggtgcgactc
     4741 ctacttgagc ggagcggatg tactgctgct gacggagctc gatatcggta tggcacgctc
     4801 cgcgaatcgc gacatccctc gcctgctggc acgggagttg ggtatgcaat acgcgttcgc
     4861 accctgttac ctgaatctga cgaagggcgc cggtgccgaa tgcgaggcgc aaggagaaaa
     4921 ccagcttggc ctgcacggga acgccattct gagccgttat ccgatcgaaa acgcgcgggc
     4981 cgttctattc ccgaatgggc gggacaaaat gcgcggccgc gaaaaacggc tcggatctca
     5041 aacggccgtg ctggcggaaa tctggttccc gggttccagc gtcacggccg ccgcggttca
     5101 tctggacgcg cagtccacgc agcggcaccg gtacgaacag atgcggttgc tgctggatgc
     5161 cgtcccgccg gaaggccccg ccatcatcgg aggagactgg aatacgagca cctacaactc
     5221 ctcacgggcg cttcacgcga ttatcggttt cgcggtccgg gtgttgatgg gcgtcggcaa
     5281 cgtgatccgg aaccactacc tgcatccgta tcaccggttc gagcgggacc tgttccagct
     5341 tctggagaat cgcggattcg attaccgctc ctcaaacctg ctgggtgttc gcaccgccgg
     5401 attctgcatt gccgatgagc gggcgaacaa gaacctgggc gagtgggttc cggcgtggtg
     5461 cttccacttt attcgatggg ctcttcgaaa ccacggcggc aagtgccctc taaagcttga
     5521 ctggtttgcg gtgcgcggac tccggtgtaa aaaccccact gtcctgcacg ccttccggga
     5581 ggagcgcgcg acgcccctct ccgatcacga ccccatcggc attgacgtgg tggtgtgata
     5641 tcagccggca acggcgcgat tttgttcgaa ctcccacgcg ctgcgcacga cggtttccag
     5701 actgctcgag cgtggcgacc agccaaggct tcgctgtagc cggtccgggt tggctaccag
     5761 cactggagga tctccttcgc gccgcggccc cacggtatag gggactctct tgcccacgat
     5821 cttttccacc gtccggagaa cttccatcac cgaatagccg gagccggtgc ccacattgaa
     5881 cacatcggat tccccgccgg acaggagaga ttcaagagcc agcacatgtg cctccgccag
     5941 gtcgcgaacg tggatgtaat cgcggatgca cgtgccatcg ggcgtatcgt agtcgtcgcc
     6001 aaagatcgta atcggcgtcc cggtcccgat ggctcggagc agcagaggga tcaaatgcgt
     6061 ctccgggtca tgctcctcgc ccagtccggc tgccgggtcc gctccacacg cgttgaagta
     6121 tcgcaggcag acgcttttca ggccacgcaa ttcgctcagc caccgcaata ccttctccac
     6181 catcactttg gattctccgt acgggctgac cggcgcatac ggtgcgtcct cgctgatcgg
     6241 taccgacgtg ggattgccgt acactgccgc agtggaggaa aacaccagcc gtttcacgcc
     6301 ggcgcgttgc atggcggtga gcagcgacag agatccggaa aggttgttgt tgaaatacaa
     6361 ctccggctcc ctggtggatt caccaacggc gatgtaagcg gcgaagtgga tcacggcttc
     6421 cacgcgcccg gcaatcatca gcgccgtcaa ggcgtccgtg tcctgcatcc ttacaacgtg
     6481 caggcgttcg ggcgcgacat tgtgcctgtg cccgcgggag agatcgtccg cgataaccac
     6541 gtcaaagccg tgctccgcca gcacatgcgc ggtgtgcgat ccgatatagc ccgcgccgcc
     6601 ggtaaccaga attcgggaca taggagaaaa gtgtcggaag agactgaata tactaggata
     6661 tcagtccatg cccaagaaac aggtccgcta ccgggacgcc ggcgtcaata tcgacgaagc
     6721 cgatcgtgcc gtttcccgca tcaaaaagct cgcacgcgcg acgttcacga aaggcgttct
     6781 aaccgacatc ggctctttcg gagccggatt tcagctcgat ggttggaaga agcccgtgct
     6841 ggtcagctcg gcggatggag ttggaacaaa actgaaggtg gcgtttctca ccggacagca
     6901 cgacaccgtc ggcgaagatc ttgtcaatca ctgcgtgaac gacatcctgg tgcagggcgc
     6961 gcggcccctg ttcttcctcg actatttcgc catcgggaag ctggatgccg aggtggccga
     7021 gcaggtggta acgggcattt cccgtggctg ccggctgaac ggttgcgccc tgatcggcgg
     7081 cgaaaccgcc gagatgccgg gattgtacgc accaggcgaa tacgatctcg ccgggttcat
     7141 tgtcggcgcc gtggagcgcc gcgcgctgat caccggcggc aacatcaaac cgggggatgt
     7201 tctgatcggg ctgccttcga atggcctgca cacaaacggg tactccctcg ccagaaagct
     7261 cctgttcgac gtcgccggtt acgggcctaa gacttttcta ccggagctgc aatcgagcgt
     7321 cggggaagag cttctcaaag tgcaccggag ctaccggaaa ccgatgctcg cattgctgga
     7381 agcgggattg ttgaagggtg cggcacacgt caccggaggc ggcatcagcg acaacctgcc
     7441 ccgcgtgctg ccgaagggat tggctgcgcg tatcaatgcg gcttcgtggc cggtcctgcc
     7501 catcttcgag gtgttgaaac gcgtcggcaa tataccggaa gaggactatc gccggacgtt
     7561 caatctcggc ttgggcatga ttctggcgat cggctcgcgt aaactccgtg ccgccgtacg
     7621 gcttctcgat gcattgaagg agccttatca ccagattgg
//
LOCUS       DASTMK010000608        14306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_190731, whole genome shotgun sequence.
ACCESSION   DASTMK010000608 DASTMK010000000
VERSION     DASTMK010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190731"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1467
                     /locus_tag="VFQ79_25040"
     CDS             313..1467
                     /locus_tag="VFQ79_25040"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0143011.1"
                     /translation="MTAKTAIRKQFILNPAALEQLFSVLESQEFQIVGPTIRNGTIRY
                     DYIKHCEELPSGWTSEHERASCRLKRREDDAVFGYVPGADSLKRFLHPPELRLFRAER
                     ENGVFRILDNPAPAPKLAFIGVRSCELRALSILDRVLAEDRYPDAVYVASRSRVFIVA
                     VNCNEPAPTCFCASMGGGPEPAGGFDLLLTEIASDNQHVFVAEAGTSEGSGVLAALNP
                     GEVSKEKKAALRETMDRSKAAFKRKVETEGLRDFLYESFDHPQWDRVAAQCFLCGNCT
                     MVCPTCFCVTVEDSTDVPVRRAERWRKWDSCFTQNFSYIHGGSVRLSPKSRYRQWLTH
                     KMAAWVDQFGTFGCVGCGRCITWCPAGIDVTEQIAVLRGTAAPAASGDAG"
     gene            1485..1961
                     /locus_tag="VFQ79_25045"
     CDS             1485..1961
                     /locus_tag="VFQ79_25045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0143012.1"
                     /translation="METQTLAGTLAKHPFLVGMLPHHVDRLASMAFEVKFKQDEFIFR
                     EGDESGFFYLLLSGKVALEVQAPARTFRIQVVGEGEELGWSSLLSPARKCFQARCLEP
                     VIALAFDGVRLRGACDADSEFGYCLMKNVLGVVAERLQATRLQLLDIFKPVGSKMI"
     gene            1958..2791
                     /locus_tag="VFQ79_25050"
     CDS             1958..2791
                     /locus_tag="VFQ79_25050"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEU0143013.1"
                     /translation="MTVASVMTPLAARVARVDKETHDTFTLAMESGNGVDLPRFRPGQ
                     FSMLYLFGAGELPISISGDPERPGELVYTVRSVGQVTYALVTRKPGDSVGVRGPFGTQ
                     WPLASARGRDILIVAGGIGLAPLRPAVYHILRQRGDYGRLVLLYGARSPRDLLYKKEL
                     SAWSYLPDTQVLTTVDYGGVSWRGHVGVVTTLFRFIRLQPNQTTAFICGPELMMRYVV
                     RELETRGMPAGHIHLSMERNMKCAVGFCGHCQMGPRFICKDGPIFPYPEMRPWLDRYE
                     L"
     gene            2781..3569
                     /locus_tag="VFQ79_25055"
     CDS             2781..3569
                     /locus_tag="VFQ79_25055"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143014.1"
                     /translation="MNSDTDRKPKIAIFKFASCDGCQLSLLELEDSLLDLVNAVEIAY
                     FPEASRRMLKGPYDIGLVEGSITTHHDAERIQRVRRESKTLMTIGACATAGGIQALRN
                     WKDVGEFVRFVYAKPDFISTLKMSTPIAEHVPVDFELRGCPINKMHLVELIGAMLAGR
                     KPNIPTYSVCMECKRRGNVCLAVSSSLPCLGPVTQAGCGAICPAYHRACYGCFGPMEN
                     PNPTALAARFEAQGHADRDIMRAFRMFNAWSWQFRQVSEERERR"
     gene            3566..4897
                     /locus_tag="VFQ79_25060"
     CDS             3566..4897
                     /locus_tag="VFQ79_25060"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEU0143015.1"
                     /translation="MSTQTETGRTTVIKVDCLARVEGEGALYIKLKGDRIQDVKFKIH
                     EPPRLFEAFLRGRHIREAPDITARICGICPIAYQMSAVHAIEKALKVSPEPAIRRLRR
                     LFYCGEWIESHALHVFLLHAPDFLGYDDVIAMAREHRETVEKALRLKKIGNGIVATLG
                     GREIHPVSAAVGGFHAVPGKSELNALLPDLEWALETSLETVKLVSGLPFPEFDQDYEF
                     VSLSHPGEYPLNEGRIVSNKGLDIDVSEYEDHFLEQHVKHSNALHSLLRERGSYMVGP
                     LARLNLNFEKLPELARAVAGECGFTLPCRNPFKSIIARALEMVFACHEAIRIIGEYEV
                     PGTPCMELPVHAGTGQAATEAPRGLLYHRYVFDDAGQILAARIIPPTSQNQKRIEDDL
                     WQYIPQLLGKPEEEMTLRCEQAIRNYDPCISCATHFLRVRIDQQGEPCPRS"
     gene            4882..5337
                     /locus_tag="VFQ79_25065"
     CDS             4882..5337
                     /locus_tag="VFQ79_25065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEU0143016.1"
                     /translation="MSTLVIGCGNSGRSDDAAGLLVVRKLRAMGVHAIEQECEAMPLL
                     DKWNGYTRVILVDAVVTGKQPGELTAWDASKERVPRECFHCSTHVFGVADAIELGRIL
                     DRLPPQLSIYGIEAARLDAGSEVTVPVADAIDRVARQIRDEVVACKDPH"
     gene            5368..7512
                     /gene="hypF"
                     /locus_tag="VFQ79_25070"
     CDS             5368..7512
                     /gene="hypF"
                     /locus_tag="VFQ79_25070"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEU0143017.1"
                     /translation="MIERVRVVLTGAVQGVGFRPFVYRLAGQLQLSGWVLNSAAGLVV
                     EVEGAGSDIAVFLERLETERPPAALILTREVSALAPSGASGFRILSSDGSLDRTAGVL
                     PDLATCPACLAEISAPGDRRYGYAFTNCTLCGPRYTIVRDIPYDRPNTTMRDFALCDD
                     CRREYLDPADRRFHAQPVACPRCGPHLNGTIASAAGAILAGEIVALKGIGGFQLFADA
                     RNEETVRRLRARKGREEKPFAMLMPDLETARRYCRISPAEERLLTSAASPIVLLDPGS
                     ANDLAPGVSGNSPYLGVMLPYSPLHHLLMRECPFPVIATSGNRSDEPIAIDNGEARER
                     LAGIADFFLMHNRPVARPCDDSVVRLSRGREMVLRRARGYAPLPLLVPKPLPPVLAVG
                     AHLKNTIAIALGRQVVVSQHIGDLDTLEARLLFERTIDDLCRLYRFKPEAVACDLHPD
                     YASTAWAKASGLPVIGIQHHHAHAASCAAENALAEPYLAVSWDGTGYGLDGTIWGGEI
                     FLVEQGRFERISHLRPFRLPGGEAAVREGWRGAASVLAGIGVRSGPDAIHQMLSRDLN
                     SPWTTSVGRLFDAVASITGVAQESKFEGQAAMLLEWAIRGLDTAEAYPLPDGDWRTLV
                     EAVLAETRGGTPPQIISAKFHNSLVQWMIETAERASVPRIALSGGVFQNRYLTERAVH
                     VLESGGFRVYTHQRVPANDGGIALGQAVLAGN"
     gene            7514..7771
                     /locus_tag="VFQ79_25075"
     CDS             7514..7771
                     /locus_tag="VFQ79_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEU0143018.1"
                     /translation="MCLAVPGKIVDIGQEIDPTLRNAKVDFGGIRKEVSLAFTPEAQV
                     GDYVLVHVGFALSVVDEQEATRIFEMLKQMNELAELEPDQP"
     gene            7768..8901
                     /gene="hypD"
                     /locus_tag="VFQ79_25080"
     CDS             7768..8901
                     /gene="hypD"
                     /locus_tag="VFQ79_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEU0143019.1"
                     /translation="MKFIDEYRDRGAVRGIARNIRGLVTKPWTLMEVCGGQTHTILKS
                     GLQDLLPPEITLVHGPGCPVCVTPVELIDRAVEIASRPEVTFCSFGDMLRVPGSSKSL
                     FDAKAAGGDVRIVYSPLDALKLARADSSREVVFFAVGFETTAPANAMAAWQAAREGLK
                     NFSMLVAHVLVPPAMEAILTSANCRVQAFLAPGHVCAVVGFKEYENIAERFQVPIVVT
                     GFEPLDLLDGVRMAVAQLESGRAEVENQYSRAVRRDGNRAALERVAEVFEVSHRKWRG
                     IGEIPWSGLKLRERYAAFDAGQKFGLAESVVEEPSECISGVVLQGLRKPYECPAFGTH
                     CTPETPLGAPMVSSEGACAAYYLYGRHREERLQPELPGATSGE"
     gene            8840..9886
                     /gene="hypE"
                     /locus_tag="VFQ79_25085"
     CDS             8840..9886
                     /gene="hypE"
                     /locus_tag="VFQ79_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEU0143020.1"
                     /translation="MADTVKKDFNLSCPAPRLANDRIVLAHGGGGKLTQQLIERVFLP
                     AFADPALEARHDGASLEVAGLKLAFTTDSYVVRPLIFPGGNIGDLAVNGTVNDLAMCG
                     ARPLWLSVGFILEEGLDMEVLETVVASMAKAAETACVRLVTGDTKVVDKGKADGIFIN
                     TAGIGAIETPLPVGPARVQPGDAILLNGDIGAHGTAILSVREGLEFESPILSDTAPLW
                     GAVEKLIDAGIEIRCLRDLTRGGLSSAVNEIAQARGVGIRLNEAAIPVTEPVRGACEI
                     LGLDPLYVANEGRFIAIVPAAQAESASKLMGPDAVHIGGVVEDHPRTVVMRSKIGGNR
                     VVDMLSGEQLPRIC"
     gene            complement(9887..10228)
                     /locus_tag="VFQ79_25090"
     CDS             complement(9887..10228)
                     /locus_tag="VFQ79_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEU0143021.1"
                     /translation="MSAPTILPEKHTKGEERLEPLYNVVLLDDDEHTYDYVIEMLQTL
                     FFCSAADAFRHAVEVDTTGRTIVMTCERPEAEFARDQIHAFGRDWRMPISKGSMSAVV
                     EPAGPAGGGCN"
     gene            10761..11822
                     /gene="waaF"
                     /locus_tag="VFQ79_25095"
     CDS             10761..11822
                     /gene="waaF"
                     /locus_tag="VFQ79_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEU0143022.1"
                     /translation="MHSELAPQRILVRATNWVGDAVMSLPAVRALRKRYPASHIAVLA
                     KPWVADLYTRESSIDELISYPAARGVKDAAGKWRVARRIARERFDCAVLLPNAFEAAL
                     VAWLARIPRRIGYSRDARGFLLTDAVAVPKQGEIPPHQHYYYLELLRRAGVLASLPAV
                     PSIELEGSAQAREAGASRFREFEMPERVIGISPGAAYGSAKRWLPERFAEAAARLASG
                     GHSGVAVFGSAGERALCETVAAAVRSHGAQALNLAGKTSLGQFIELAAACSLYLTNDS
                     GAMHIASALGVPTVAIFGPTDPIATGPTGPATVVVRESVSCSPCLLRECPIDHRCMTQ
                     VTVDRVVQAALELDAHVFK"
     gene            11829..12359
                     /locus_tag="VFQ79_25100"
     CDS             11829..12359
                     /locus_tag="VFQ79_25100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143023.1"
                     /translation="MRDFSQTRVTVIGDFRTDDSEVRTIVALGGRVSAVGLATAAQKS
                     EWKRLGLDVSGIVRSRKAVAEAAVAGIAGADAVLFADWDWDDVYQAVIDAEIESALSD
                     GRFTIPIVSSANERYATLVFPDQRAAFTLARGAGASFEEAEKFSRRERGHQKQNTIRR
                     AGDRSGRKTTARRRTR"
     gene            12322..12669
                     /locus_tag="VFQ79_25105"
     CDS             12322..12669
                     /locus_tag="VFQ79_25105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143024.1"
                     /translation="MGAKLQREGERVEAVVGSFDPLVAEQARYLHQLAASGARLFAVI
                     SHPERPLLSASARAELVAALKDIDFVVLGEGDRPEEFLKCLPDGCIHRREHSDAQLTE
                     QFIRHVQGRQRTS"
     gene            12671..13654
                     /locus_tag="VFQ79_25110"
     CDS             12671..13654
                     /locus_tag="VFQ79_25110"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU0143025.1"
                     /translation="MSGPHILVVRIGSMGDVIHALPAVATLKHSFPHCRLSWVISPRW
                     APLLDGNPFVDQVIPFDRTSISGIAAAWRILRSKRCDFSVDFQGLIQSALIATIARPD
                     RIFGFHQSQIREKLAALFYSHKTQATSAHVVERNLELAVEAGGSNVLHTFPLPEGRLE
                     GDLPDGEFILASPVAGWAAKQWPAENYAKLAQLIRQELGLPLVLNGSPASGAALSAVP
                     FTHTSLTGLTGLIYATRRATAVVGVDSGPMHLAAALGKPGVAVMGPTDPSRNGPYGDS
                     FTVLRSPHAQTSYKRHAESAASMRDISPEQVLGTLKARLACRAHPTGLSQS"
     gene            13654..14211
                     /locus_tag="VFQ79_25115"
     CDS             13654..14211
                     /locus_tag="VFQ79_25115"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0143026.1"
                     /translation="MRQYWFPKPYADFVARLRVPIGFLLVAAFAWLSRPSPLSLCIGA
                     SISFAGIALRAWAAGHLSKNTELATSGPYAYVRNPLYLGTLIAALGFVIASRESSLGI
                     LFAAVFVFVYLPVVELEEQHLRKLFPEYGAYASRVKMLIPSFGYRGAGRKFQWRLYMR
                     NQEFQALLGFLAGIAFLAWKAWAAG"
BASE COUNT         2679 a         4153 c         4558 g         2916 t
ORIGIN      
        1 cagtcccgaa tacctgatgg aacagttccg cgcggcggcg gccggaacaa ttgtcggaaa
       61 ggcagaactt cgaattgaag tatcaaccga tgagcattct ccccgcgcac acgaattgtt
      121 gctgatcacg gtggagttag agcgttgagc cattgcgcgc ggtacgaagg gagcgggtgc
      181 gtcaattggc gataaaaaga tctgaaaatc gtgcaatttc acccactacg ttctgtgggt
      241 gagggaactt cagtgttctt agttacttaa ctgtggtacg ccaagtgctg actctgtggc
      301 acggcggtta tcatgacggc caaaactgca atacggaagc agtttattct gaatccggcg
      361 gccctcgaac agctcttctc cgttcttgaa tcccaggaat ttcaaatcgt cggtcctacc
      421 atcaggaacg gaaccattcg gtacgactac ataaaacatt gcgaagagct gccctccgga
      481 tggaccagcg aacacgaacg ggcatcctgc cgtctgaaaa ggcgcgagga tgatgcggta
      541 ttcgggtatg tgcctggtgc ggacagcttg aagcgctttc tgcacccgcc ggagttgcgt
      601 ctgttccgag ccgagcggga gaacggcgta ttccggattc tcgacaatcc ggcgcctgcg
      661 cccaagctcg cttttattgg tgtgcgaagc tgtgagctcc gcgcgctcag catactcgac
      721 cgggtcctgg cggaagaccg gtatcccgac gcggtatacg tcgcgtcgcg cagccgggtt
      781 ttcatcgtgg cggtgaattg caacgagcct gcgcccacct gcttttgtgc gtcgatgggc
      841 ggcggtccgg aaccggccgg tgggttcgac ctgttgctga ccgagattgc ctcggacaac
      901 cagcacgttt tcgtggccga agcgggcact tccgaaggtt ccggcgtgct tgcggccctg
      961 aatcccgggg aggtttccaa ggaaaaaaaa gcggcactgc gtgaaacgat ggaccgctcc
     1021 aaagcggcct tcaaaagaaa ggtggagacg gagggtcttc gggatttcct ctacgagagt
     1081 ttcgaccatc cacagtggga ccgcgtggcc gcacagtgct tcctatgtgg aaactgcacc
     1141 atggtgtgcc cgacctgttt ctgcgtcacc gtcgaagaca gcacggacgt accggtccgg
     1201 cgggccgaac ggtggcggaa gtgggattcc tgttttaccc agaatttctc ttatatccat
     1261 ggcgggagcg tgcgcctctc gccgaagtcc cgctacaggc agtggctgac tcataagatg
     1321 gctgcctggg tcgaccagtt tggaactttt ggatgtgtcg gatgcggccg ctgtatcacc
     1381 tggtgtcccg ccggcattga cgttaccgaa cagatcgccg tcttgcgcgg gacagcggct
     1441 cccgcggcat ccggcgacgc tggttgagga ggaggtattg ggaaatggaa acgcagaccc
     1501 tcgcgggtac actggcaaaa caccccttcc ttgtggggat gttgcctcac cacgtggacc
     1561 ggctggcgtc catggcgttc gaggtgaaat tcaaacagga cgaattcatc tttcgtgaag
     1621 gggacgagtc cggctttttc tatctgctgc tgtccggaaa ggtcgctctg gaagtgcagg
     1681 cgccggcgcg aacgtttcga atccaagttg taggagaagg cgaggagttg ggctggtcat
     1741 cgctgctttc cccggcgcga aagtgttttc aggcacgatg cctcgaaccg gtgatcgcac
     1801 tcgcttttga cggagtgcgt ttgcgcggcg cctgcgacgc cgatagcgaa tttggctact
     1861 gcctgatgaa gaacgtactg ggtgtcgtgg ctgagcgcct gcaagcgaca cgcctgcaac
     1921 tgctcgatat ttttaagccg gtcgggagca aaatgatatg acggtagcga gcgtaatgac
     1981 accgctcgct gcacgagtcg cgcgagtcga taaagagacg cacgatacct tcacgctggc
     2041 gatggaatcg ggcaacggag ttgatttacc gcgctttcgt cctggccagt tcagcatgtt
     2101 gtatctgttt ggggccggcg agctgccgat ttcgatcagc ggcgatccgg aaaggcccgg
     2161 cgaactggtc tatacggtgc gttcggtcgg gcaggttact tacgccctgg ttactcgcaa
     2221 gcccggagat tcggtgggag tgcgcggtcc tttcggcacc caatggccgc tggcgtccgc
     2281 gcgtggccgc gacatattga ttgtcgccgg gggcattggc ctcgcgccgc tgcggcccgc
     2341 ggtctatcac atcctgaggc agcgaggcga ttacggccgg ctcgttcttc tctacggcgc
     2401 gcgcagtccc agggatcttc tgtataaaaa agaactctcc gcctggtcgt atctgccgga
     2461 tacgcaggtg ttgaccacgg tcgactatgg cggcgtgagc tggcgcggtc atgtgggcgt
     2521 ggtaacgaca ctgttccgct tcatccgcct tcagccgaac cagacgacgg cattcatttg
     2581 cgggccggag ctgatgatgc gctacgtggt gcgggaactg gaaacgcgcg gcatgccggc
     2641 cgggcacatt cacctgtcga tggagcgcaa catgaagtgc gcggtcggtt tctgcggcca
     2701 ctgccagatg gggccgcgct tcatctgcaa agacgggccg atcttccctt atccggagat
     2761 gcggccatgg ctggaccgtt atgaactctg atacggacag gaagccgaag atcgccattt
     2821 tcaaattcgc atcctgtgac ggctgccagt tgagcctgct ggagctggaa gactcgctgc
     2881 tggacctggt gaacgccgtc gaaatcgcct acttcccgga ggcgagccgc aggatgctga
     2941 aggggcctta cgatatcggc ctggtggaag gctccatcac cacgcaccac gacgcggaac
     3001 gcatccagcg ggtccgccgc gaatcgaaga cccttatgac tatcggggcg tgcgccaccg
     3061 ccggaggcat acaggcgctg cggaactgga aagacgtcgg tgaattcgtg cggttcgtct
     3121 acgcgaagcc cgattttatc agcacgctga agatgtccac tccgatcgcg gaacatgttc
     3181 ccgtagactt tgagctgcgg ggatgcccca tcaacaagat gcacctggtg gaactgatcg
     3241 gagccatgct cgccggcagg aagcccaaca ttcccaccta cagcgtgtgc atggaatgca
     3301 aacggcgggg caacgtctgc ctcgccgtca gctccagcct gccttgcctg ggacctgtga
     3361 cgcaggcggg ttgcggcgca atctgcccgg cgtaccatcg cgcctgttac gggtgcttcg
     3421 gaccgatgga aaatcccaat cccacggctc tggccgcgcg cttcgaggcg cagggccacg
     3481 ccgaccgcga catcatgcgc gcgttccgca tgttcaatgc ctggtcctgg caattccgcc
     3541 aggtgagcga agaaagggag cggagatgag cacgcagaca gagaccggcc ggacgacggt
     3601 catcaaggtg gattgtctgg ccagggtgga aggcgaaggg gccctttata tcaaactcaa
     3661 gggcgaccgc attcaggacg tcaagttcaa aatccacgag ccaccccgtc tgtttgaagc
     3721 ctttctgcgg gggcggcaca tccgggaggc ccccgacatt acggcgcgca tctgcggcat
     3781 ctgccccatc gcgtatcaga tgagtgccgt tcatgcgatc gaaaaggcgc tgaaagtatc
     3841 gccggagcct gccattcgaa ggctgcggcg gctgttttac tgcggagaat ggatcgagag
     3901 ccacgcgctg cacgttttcc tgctgcatgc gccggatttt ctgggctacg acgacgtgat
     3961 cgcgatggcg cgcgagcacc gcgaaacggt cgaaaaagcg ttgcgcctga aaaaaatcgg
     4021 aaatgggatt gttgcaaccc tgggcggccg ggaaatccat ccggtttccg cggcagtggg
     4081 aggctttcat gcggtccccg gcaagtccga actgaacgcg ctgctgccgg atctcgaatg
     4141 ggcgctcgaa acgtcgctcg aaaccgtgaa gctcgtttcc ggactcccct ttcccgagtt
     4201 cgatcaggac tacgaatttg tctctctttc gcaccccggt gaatatccgc tgaacgaagg
     4261 caggatcgtc tccaacaagg ggcttgatat cgatgtctcc gagtacgaag accatttcct
     4321 ggaacagcac gtcaaacact ccaacgcctt gcattcgctg cttcgcgagc gggggtctta
     4381 catggtgggt ccgctggccc gcctcaacct gaatttcgaa aagctccctg agttggcgcg
     4441 agcggttgcc ggcgagtgcg ggttcacgct gccgtgcagg aatcctttca agagcatcat
     4501 cgcgcgtgct ctcgagatgg tattcgcctg ccacgaagcc attcgaatta tcggtgaata
     4561 cgaggttccc gggaccccgt gcatggaact gccggttcac gccggaaccg gacaggcagc
     4621 cactgaagcg ccccggggcc tgctgtatca ccgctacgtg ttcgacgacg ccgggcagat
     4681 cctggcagcg agaattattc cgccgacttc ccaaaatcag aagcgcatcg aagacgatct
     4741 gtggcagtac atcccacaat tgctcggaaa gcccgaggaa gagatgacac tccggtgcga
     4801 gcaggccata cgcaactacg acccgtgcat cagttgcgcc acgcactttc tccgcgtgcg
     4861 gatcgaccag caaggagagc catgtccacg ctcgtgatcg gctgtgggaa ctccggcaga
     4921 agcgacgatg ccgccgggtt gctggtggtg cggaaactgc gcgccatggg tgtccacgcg
     4981 atcgagcagg aatgcgaggc gatgccgctg ctcgataagt ggaacgggta cacgcgtgtg
     5041 atactggtgg atgctgtcgt gacgggtaaa cagcccggtg aactgacggc atgggacgct
     5101 tcgaaggaga gggtgccccg cgagtgcttc cattgttcca cacacgtttt tggcgttgcc
     5161 gatgcgatcg aactcggtcg cattctggac cggttgcccc cgcaactttc gatttacggc
     5221 atcgaggcgg cgcggctcga tgctggatcg gaggtgacgg tgccggtcgc ggatgctatc
     5281 gaccgggtag cccgccagat tcgagacgag gtcgtcgctt gcaaggaccc tcactgaccc
     5341 gccgtttccg tcaaaccaga gcgttgagtg attgagaggg tccgagtcgt actgacgggc
     5401 gcggtgcagg gcgtgggctt caggcccttc gtttacaggc tggccgggca gttgcagctc
     5461 tccggttggg ttctgaattc agcggcgggg ctggtggtgg aggtagaagg cgccggctcc
     5521 gatattgccg tgttcctgga gcgtctggaa acggagcgcc ctcccgcggc tttgattttg
     5581 acccgggaag tgagcgcatt agcgccttcc ggcgcgagcg gttttcgcat tctctcgagc
     5641 gacggttcac tcgaccgtac ggccggagtg ttgccggatc tcgcaacctg tccggcgtgc
     5701 ctggccgaaa tttccgcacc cggcgaccgc cgatatggct acgccttcac aaactgcacg
     5761 ctctgcggcc cgcgctacac aatcgtgcgc gatatcccct acgaccggcc gaacaccacg
     5821 atgcgcgatt ttgcgctctg tgacgattgc cgccgcgaat atctcgatcc cgcggaccgg
     5881 cgtttccatg cgcagcctgt cgcctgcccg cgctgcggcc cgcatttgaa cggaaccatc
     5941 gcttcggcgg ccggggcgat cctcgccggg gagattgtag cattgaaggg catcggaggc
     6001 ttccagttgt tcgccgatgc acgcaacgaa gaaaccgtcc gccgccttcg ggcgcgaaag
     6061 gggcgtgaag agaagccgtt cgcgatgctg atgccggacc tggaaaccgc ccgccggtat
     6121 tgccgcatca gtcccgcgga ggagcggctg ctcacgtcgg cggcttctcc catcgtgttg
     6181 ctcgatccag gcagcgctaa cgaccttgcg ccgggagtat cgggaaactc accgtatctg
     6241 ggtgtgatgc tgccttactc cccgctccat cacctgctga tgcgggagtg cccgttcccc
     6301 gtcatcgcca ccagcggcaa tcgcagcgat gagcccatcg ccattgataa cggcgaggcc
     6361 cgggagcgac tggcgggcat cgccgatttt tttctgatgc acaaccggcc cgtcgcgagg
     6421 ccctgtgacg attcggtggt ccgtttgtcg cgcggccgcg agatggtgct gcggcgcgcg
     6481 cgcggatatg cgccgctgcc gttgcttgtc ccgaagccgc tgcctccggt tttggcggtg
     6541 ggcgcccatt tgaagaacac cattgctatc gcactgggcc gtcaggtggt ggtgagccag
     6601 catatcggcg atctcgatac gctcgaagcg cggctgctgt ttgaacgcac catcgacgat
     6661 ctttgccgcc tgtaccggtt taagcccgaa gcggtggcat gcgacctgca tcccgattac
     6721 gcctccaccg cgtgggcgaa ggcttcgggc ctgcctgtca tcggcattca acaccatcac
     6781 gctcacgcgg cgtcgtgtgc cgccgagaac gcgctcgcag agccgtatct tgccgtgtct
     6841 tgggatggca cgggttacgg tctggacgga acgatatggg gcggcgagat cttcctcgtc
     6901 gaacagggac gcttcgagag gatctcgcat ttgcggcctt tccggcttcc gggcggggaa
     6961 gcggctgtcc gggaaggctg gcgcggcgcc gcaagcgtgc tggccggcat cggcgtgcgt
     7021 tcgggtccgg acgccattca ccaaatgctg tcgcgtgacc tgaactcgcc ctggaccacc
     7081 agcgtcggcc gtttgttcga cgcggtggcg tctattaccg gcgtggctca ggaaagcaaa
     7141 ttcgagggac aggcggccat gctgctcgaa tgggccatcc ggggcctgga cacggcggaa
     7201 gcataccccc tgccggatgg tgactggcgg acacttgtag aggcggtatt ggctgaaacc
     7261 cgcgggggaa ccccgccgca gatcatctcg gcgaagttcc acaatagcct cgtacaatgg
     7321 atgatagaga cagccgaaag agcctcggta ccccggattg ctctcagcgg gggcgtgttc
     7381 cagaatcgat atctcacgga acgtgccgtc catgtgctgg aatccggcgg gtttcgagtg
     7441 tatacgcatc agcgggtccc tgccaacgat ggcggtattg cgctgggcca ggccgtcctg
     7501 gcgggtaatt gagatgtgtc ttgccgttcc cggaaaaatc gtcgacatcg ggcaggagat
     7561 cgatccgaca ctgcgcaacg cgaaagtcga tttcggtggc attcgcaagg aggtgagcct
     7621 tgcgttcacg ccggaggcac aggtaggcga ctatgtcctg gttcacgtcg ggttcgcctt
     7681 gagcgtagtg gacgagcagg aagcgacgcg gatcttcgag atgctgaagc agatgaatga
     7741 gctcgccgag ttggagccgg atcagccgtg aagtttatcg acgagtatcg ggaccgaggc
     7801 gcagtccggg gaattgcgcg caatatccgc ggtttggtta caaagccgtg gacgctgatg
     7861 gaggtttgcg gcgggcagac tcatacgata ttgaagagtg gccttcagga tctgctcccg
     7921 ccggagatca ccctcgtgca cggcccgggg tgtccggtct gcgtgacgcc ggtcgagctg
     7981 atcgaccgcg ctgtcgaaat tgcttcgcgt ccggaagtga cattctgttc ttttggcgac
     8041 atgctgcgcg tgcccggttc atcgaagagc ctcttcgatg cgaaggccgc cggtggggac
     8101 gtgcggattg tctattcgcc gctcgatgcc ctgaaactgg ctcgcgcgga ttcttcccgc
     8161 gaagtcgtgt tttttgccgt ggggtttgag acgaccgcgc ccgcgaacgc catggcggcc
     8221 tggcaggcgg cgcgcgaagg gctgaagaat ttttccatgc tggtggcgca cgttttggtt
     8281 ccgcccgcga tggaagcgat tctgacttcc gcgaattgcc gcgtgcaagc gtttctggcg
     8341 cctgggcacg tttgcgcggt ggtgggattc aaggaatacg agaacattgc cgaacgcttc
     8401 caggttccga tcgtggtcac gggattcgag ccgctggacc tgttggatgg ggttcgcatg
     8461 gcggtggccc agctcgaatc gggacgcgcc gaggtcgaga accagtacag ccgggcggtg
     8521 cgccgcgatg gaaatcgagc ggccctggaa cgcgttgccg aagtgttcga agtgagccac
     8581 cggaagtggc gcggcatcgg cgaaatccct tggagcggcc tgaagctgcg agagcggtac
     8641 gccgcattcg acgccgggca gaagttcggt cttgcggaga gcgtagtgga agaaccctcc
     8701 gaatgcatca gcggggttgt gctgcaaggc cttcggaaac cctacgagtg ccccgcgttc
     8761 ggaacgcatt gcacgcccga gactccgctg ggagctccca tggtctcctc cgaaggcgcc
     8821 tgcgccgctt attatctcta tggccgacac cgtgaagaaa gacttcaacc tgagctgccc
     8881 ggcgccacgt ctggcgaatg accgcattgt gctggcgcac ggcggcgggg ggaagctgac
     8941 gcagcagctc attgaacgcg tcttcctgcc cgcgttcgcc gacccggcgt tggaggcgcg
     9001 gcacgatggc gcatccctcg aagtggccgg gctgaagctc gccttcacga ccgattctta
     9061 cgttgtccgg ccgctgatct ttcccggggg caacatcggc gacctggccg tgaatggcac
     9121 cgtgaacgac cttgccatgt gcggtgcgcg gccactttgg ctgagcgtgg ggttcattct
     9181 ggaagaaggt ctcgacatgg aagtgcttga gacggttgtc gcatccatgg cgaaggctgc
     9241 ggaaacggcc tgcgtccggc tggtgaccgg cgatacgaaa gtggtagata aaggcaaagc
     9301 cgatggcatc ttcatcaata ccgccggcat aggagccatt gagacgccgc tacccgttgg
     9361 accagcccgg gttcaaccag gcgatgcgat tctcctcaac ggcgatatcg gagcgcatgg
     9421 gaccgccatt ctgtccgttc gtgaaggtct ggaattcgaa tcgcccattc ttagcgacac
     9481 ggcgccacta tggggcgcgg tggagaagtt gatcgacgcc ggtatcgaga tccgttgcct
     9541 ccgcgatctc actcgcggcg gattgtcgtc ggccgtgaac gagattgcgc aagcgcgcgg
     9601 cgtgggcatc cggttgaacg aagccgctat tcccgtcacc gagcccgtgc gcggcgcttg
     9661 cgaaattctc gggcttgacc cgctctatgt cgcgaacgaa gggcgcttca tcgccatcgt
     9721 accggcagcg caagcggaga gcgcctcgaa gctgatgggc ccggatgcgg tgcacatcgg
     9781 cggcgtggtg gaagatcacc cgcgaacggt tgtgatgcgg agcaaaatcg gcggaaaccg
     9841 cgtggtggac atgctcagtg gagagcagct tccccgaatc tgttgatcag ttgcagccgc
     9901 cgcctgccgg accggccggt tccaccaccg cgctcatgga gcctttcgag atcggcatcc
     9961 gccagtcgcg gccgaaggca tggatctggt cgcgtgcgaa ttccgcttcg ggccgctcgc
    10021 aggtcatcac gattgtccgg ccggtggtgt ccacttccac cgcgtggcgg aacgcatccg
    10081 ccgcggagca gaagaaaagc gtctgtagca tttcgataac gtagtcgtac gtgtgttcgt
    10141 cgtcatcgag aagtacgacg ttgtatagcg gctcgagcct ttcttctccc tttgtgtgct
    10201 tttccggcag aatcgtcggg gctgacatcg tgacttgctt ctacccagtc tattgaaaaa
    10261 aatagctcat gtgggaagca cagaggcagc agacggacgc ggtttccgtc ttcacattaa
    10321 gatctgatca tgcttacgct gctcagcact acggggccta taataaaaac gtggccagag
    10381 gttgggaaag caagtcagtc gagtcgcaaa tcgagtcggc tgaaagcagt agggccccac
    10441 tagcaaacga aaagctcacc tcagaacgat ctgaacttct ccgaaaaaaa gagaccctgc
    10501 tactttcacg cactcgagtg ctgaacgata tgcactcatg cacgcatgag cgctaccggg
    10561 tgattcttga gaacgcattg gcacatttgg aaaaccaact cgcagaactc gaggaaaacc
    10621 gggtagccac cgcgccaaaa accgctaccg ccgtagggta agccttccgg cgtttccgcg
    10681 gcctggatcc ggcaatgtgc gtggcgtcag gaaccgtggc ggtacgcaca tcggctaacg
    10741 tgtagttgtt agcattgaat ttgcacagcg aattggcacc gcagagaatc ctcgtccggg
    10801 ctaccaactg ggtgggcgat gccgtgatgt cgctccccgc agttcgcgcc cttcggaagc
    10861 gataccctgc atcgcacatt gctgtactgg caaagccgtg ggtggcggat ctctacactc
    10921 gcgaatcgtc catcgatgag ttgatctctt atcctgctgc ccgcggtgtg aaggatgccg
    10981 cggggaaatg gcgggtcgcg cgccgaatcg ctcgcgaacg gttcgattgc gccgttctac
    11041 tgccgaatgc cttcgaagcc gctctggtcg cctggctggc ccgcatcccg cgccgcatcg
    11101 gatacagtcg agatgcgcga gggtttctgc tgacggatgc ggtggctgtt ccgaagcagg
    11161 gcgaaatccc tccgcaccag cactattatt acctggagtt gttgcggcgc gccggcgttc
    11221 ttgcttcgct cccggctgtg ccgtcgattg agctcgaagg ctcggcacag gcacgcgagg
    11281 caggtgcgtc gaggtttagg gaattcgaga tgcccgagcg cgtgattggc atcagccccg
    11341 gagccgctta tggctcggca aagcgctggc tgcccgaacg gttcgcagaa gcggccgcgc
    11401 gcctcgcctc cggtggccac tccggcgtgg cggtttttgg ttctgccggg gagcgcgcgc
    11461 tttgtgaaac cgtagccgcc gcggtgcgta gccatggagc ccaagccctg aacctggcag
    11521 gtaagacaag cctcggccag ttcatcgagc ttgccgccgc ctgtagcctg tatctgacaa
    11581 acgactccgg agccatgcac atcgcctctg cgctgggagt tcccactgtc gcgatattcg
    11641 gtccgaccga tcccatcgca acaggtccca cgggcccggc aacggtcgtc gttcgggaaa
    11701 gcgtatcttg cagcccctgc ctgttgcggg aatgcccgat cgaccatcga tgcatgactc
    11761 aggttacggt ggatcgtgtc gtgcaagccg cgttggaact ggatgcccat gtgttcaaat
    11821 aggtaacagt gcgcgacttc tcccagacac gggtgacggt tatcggcgac tttcgaacgg
    11881 acgattcgga agtgcgaacc atagttgcgc tgggcggcag agtgtcagcc gtgggccttg
    11941 ctaccgcggc ccagaaaagc gagtggaagc ggttgggcct ggatgtgagc ggcattgtgc
    12001 ggtcgcgtaa agcggtcgcc gaagcggctg ttgcgggcat agccggagcg gacgcggtgt
    12061 tgtttgccga ctgggattgg gatgatgtct accaggctgt gatcgatgcc gagatcgaat
    12121 cggcattatc ggatggacgg tttacgattc ccatcgtttc ttcggccaat gagcggtacg
    12181 ccacgctggt cttcccagat cagagggctg cgtttacgct cgcgcgcggc gcgggtgcat
    12241 cttttgagga ggcagagaag ttttcgcggc gagagcgtgg acaccagaaa caaaatacta
    12301 tccgtcgagc aggcgatcgc agtgggcgca aaactacagc gagaaggcga acgcgttgag
    12361 gcggttgttg gctcgttcga tccgctggtg gcggagcagg cgcgctattt acaccagctt
    12421 gccgcctccg gcgcgcgtct gttcgcagtg atctcccatc ccgagcggcc cctgttgtcc
    12481 gcgagcgcac gcgcggaact ggtggcggct ttgaaggata tagactttgt tgtgttgggc
    12541 gagggggatc gtccggagga gtttctgaag tgcctgccgg atggctgcat ccaccgccgc
    12601 gagcattccg atgctcaact gaccgagcag ttcatccgcc acgtacaggg gcggcagagg
    12661 acaagctgac gtgtctggac cacacatcct ggtggttcga atcggctcga tgggcgacgt
    12721 catccacgcg ttgcccgcgg tcgcgacgct gaagcatagt tttccgcact gccgtctgag
    12781 ctgggtgatt tcaccgcggt gggcacccct gctggacggt aatccatttg tcgaccaggt
    12841 cattcccttt gaccgcactt cgatttccgg gatcgcggcg gcctggcgga ttttgcgaag
    12901 caaacgttgc gacttctcgg tggacttcca gggcttgatt cagtctgcac tgatcgccac
    12961 catcgcgcgg ccggatcgaa ttttcggttt tcatcaatct caaatcaggg aaaagttggc
    13021 agcattgttc tattcgcaca agacgcaggc tacatcggcg catgtcgtag aacgtaatct
    13081 ggagctcgcc gtggaggcgg gaggctcgaa tgtactgcac acgtttcctt tacccgaagg
    13141 ccgcctcgag ggagatttgc cggacggcga gttcatactg gcgagtccgg ttgccggatg
    13201 ggccgccaag caatggccag ccgagaatta cgcgaagctc gcacaactga tccgccagga
    13261 actgggattg cccctggtgt tgaacggatc gcctgcttcg ggcgcggcgc tgagtgcggt
    13321 tccgttcacg cacacatcgt tgacgggtct caccgggctg atttatgcca cgcgccgagc
    13381 gacggcggtt gtgggcgtcg atagcgggcc gatgcacctg gccgcagcat tgggaaaacc
    13441 gggagtggcc gtgatgggcc ctaccgatcc ctctcgcaat gggccgtatg gcgacagctt
    13501 caccgtgctc agaagcccac acgcgcaaac ctcctacaag cgccacgcag agagcgcggc
    13561 cagcatgcgc gatatctcgc ccgaacaggt tctgggtacg ctgaaggcac ggctggcgtg
    13621 ccgcgcgcat cctacgggtc tttctcagag ttgatgcgcc agtactggtt ccctaaaccc
    13681 tacgcggatt tcgtcgcgcg gctgcgcgtg ccgatcggct tcctgctggt cgcggcgttc
    13741 gcctggcttt cccggccaag cccactctca ttgtgcatag gcgcctccat ctcttttgcc
    13801 ggaattgcgc tgcgtgcctg ggccgccgga catctttcca agaacacaga gcttgccacc
    13861 agcggcccct acgcgtatgt tcgaaatccg ctgtacctcg gaacgcttat cgccgcgctt
    13921 ggtttcgtga tcgcatcgcg cgaaagcagt ctcggcatcc tctttgccgc tgttttcgta
    13981 ttcgtctacc tgcctgtggt ggaactggaa gaacaacatc tccggaagct gtttccggaa
    14041 tacggcgcct atgcatcgcg cgtgaaaatg ctgatcccat cattcggcta tagaggtgcc
    14101 ggacgaaaat tccagtggcg gctctatatg cggaatcagg aatttcaagc gctactcggt
    14161 tttctcgcgg gcatcgcatt tctggcctgg aaagcctggg cagcaggata accggccgcc
    14221 tactgcttca gcttttcaag cagcttttga acgtgcggcg tcctctccga gtcgggatgc
    14281 ccttcgatga agtccttcag ttccgc
//
LOCUS       DASTMK010000609         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_383284, whole genome shotgun sequence.
ACCESSION   DASTMK010000609 DASTMK010000000
VERSION     DASTMK010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383284"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFQ79_25120"
     CDS             complement(<1..310)
                     /locus_tag="VFQ79_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143027.1"
                     /translation="MDPEKRAGDARAGVLDELQRINDQIDRAVELAGLKPLFYRLETI
                     AQEYHGDAEVEAMAVLARQRLIVKGSRMRDQPPGDPGAAESSPIPTPFPTAAPPVVSD
                     V"
     gene            438..1802
                     /locus_tag="VFQ79_25125"
     CDS             438..1802
                     /locus_tag="VFQ79_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU0143028.1"
                     /translation="MIIDTNLTAPALESGMERLFEAAAAKIRSIEASWNPSSGSPVFT
                     SGGQYTSRGWTEWTQGFQFGCAILEFDATGEQIFLDIGRQRTVDIMAPHITHTGVHDH
                     GFNNVSTYGNLLRLMQEGRIESNPWERRFYELALKCSGAVQAARWTNIKDGGGYIYSF
                     NGPHSLFVDTMRTIRALAVSHHLGHALLAENDQHISLLDRLIQHAHATAQYSVYYGEG
                     RDAYDIRGRVAHESVFNTNDGNYRCPNSQQGYSPFSTWTRGLAWAMCGFAEQLEFLAT
                     VGDDELTPHGGRSTVEAFMRKAAEAVCDFYIENATASDGIPYWDTGAPGLANLKDWRS
                     RPSDPFNDHEPVDSSAAVIAAQGLLRLGRYLKNDRYWNAGLTVCRTLFAQPYLSTDPN
                     HQGLILHSIYHRPNGWDKIPDGRKIPSGEACMWGDYHAMELALYVQRILTNKPYYKFW
                     SETQ"
     gene            1799..2248
                     /locus_tag="VFQ79_25130"
     CDS             1799..2248
                     /locus_tag="VFQ79_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0143029.1"
                     /translation="MIIADLSQIAGRTYPARRRTQNLVGGASPIQATNFSIGHVTLEP
                     DGGQVPWHNQEQEEIYFIIEGTAEMCLGEERQTLSTGQAVYIPSKVFHQLTNTGSTPC
                     RMLYCYGPAGDVAHWRQELEGTLPKAGVDMPPLPAGARPQCTDRPKD"
     gene            2255..3403
                     /locus_tag="VFQ79_25135"
     CDS             2255..3403
                     /locus_tag="VFQ79_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0143030.1"
                     /translation="MTTHKIGIIMNGVTGRMGLNQHLRRSIYDIMQQGGVRTRDGVIM
                     PAPLLVGRNAAKLQAISAECGGLPWTTDLDSALADPQYTVYFDAQTTNRRAPDVSKAI
                     KAGKHIYCEKPTSDSLEVALDLYRQAKAAGVKNGVVQDKLWLPGLLKVKTLDQLGFFG
                     DILSVRGEFGYWVFEGDKVPAQRPSWNYRKEEGGGIIIDMLCHWRYVLDNLFGEVKSV
                     SCLGATHIPTRWDEDDKPYACTAEDSVYATFQLASGVIAHFNSSWVVRVRRDDLLTLQ
                     IDGTKGTAVAGLRDAWIQPYAATPRPVWNPDIPQPLSFFDGWQKVPEQTGYSNAFKVQ
                     WELFLKHVVTGEPFPWDLLAGAKGVQLAEKGIESWHKRCWVDLDPLEA"
     gene            3434..3844
                     /locus_tag="VFQ79_25140"
     CDS             3434..3844
                     /locus_tag="VFQ79_25140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143031.1"
                     /translation="MEQPQEADQPQPAAVAAREDSTIRLLQARAIRVAYVLVFLISFG
                     AGLIGWYLIGGPGHFEIMGWHVKLVLSVAFAAAVTKLARASTLEDRAWNRRSLRWLGY
                     VITLAVLMATATYYYHLQEPIEEEMVPEESESPL"
     gene            4325..>4645
                     /locus_tag="VFQ79_25145"
     CDS             4325..>4645
                     /locus_tag="VFQ79_25145"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0143032.1"
                     /translation="MRPVIFHPKARDEIRRFPKEPRARLGRGLYRLQMGESLSMPNAR
                     PMATIASGVWELRVRGEDGIFRVFYYTTSGAGVLVFHAFAKKTQRTPASEIELARKRL
                     KELLD"
BASE COUNT          999 a         1366 c         1341 g          939 t
ORIGIN      
        1 taacgtcaga gacgacagga ggcgccgcag ttggaaaagg cgtcgggatt ggagaagact
       61 cagcggcacc tggatcgccc ggaggctgat cccgcatccg gcttcctttc acaatcaggc
      121 gctggcgtgc cagtaccgcc atggcctcta cttcagcgtc cccgtgatac tcctgcgcta
      181 tggtttccag cctgtagaaa aggggtttga gcccggccag ctcaaccgcg cgatcgatct
      241 gatcgttaat acgctgcaat tcatcaagca caccggcccg ggcgtcgccc gccctcttct
      301 ctggatccac tctcactctc ctcgaatacg atggctcacc attaatttac tagggcttgc
      361 gaggtagttt ccactggaca ggcagcgctt ggctgaagat cgccgtcata gaagcttaag
      421 ataagtactt acacttcatg atcattgata ccaacctcac agctcccgcg ctcgagtccg
      481 gtatggaacg cctgtttgaa gcagccgcgg caaagatccg gtccatcgaa gcgtcgtgga
      541 acccatccag cggctctccc gtttttacct cgggcggcca atacaccagc cgcggctgga
      601 cggaatggac tcagggtttt cagttcggct gcgcaattct cgaattcgac gcaactggtg
      661 agcagatctt tctggatatc ggccgccagc ggacggtaga catcatggcg ccgcatatta
      721 ctcataccgg cgttcacgat catggcttca ataacgtcag cacttatggc aatctgcttc
      781 gcctgatgca ggagggccgg atcgaatcga acccatggga acggcgcttt tatgagctgg
      841 cgctgaaatg ctccggcgcc gtgcaggctg cccgctggac gaacatcaag gacggaggcg
      901 gctatatcta ctccttcaac gggccgcatt cgctgtttgt cgacaccatg cgcactatcc
      961 gcgccctggc cgtcagccac cacctgggac acgcactact cgccgagaac gaccagcaca
     1021 tttccctcct cgaccggctc attcaacacg cgcacgcgac agcacagtat tcggtttact
     1081 atggcgaggg ccgcgacgcg tacgacattc gcggccgtgt tgcgcacgag agtgttttca
     1141 atacgaacga cggcaattac cgctgtccga actcgcagca gggatattcg cccttcagca
     1201 cgtggacccg cggactcgcc tgggccatgt gcggtttcgc ggaacagctc gagtttcttg
     1261 cgacggttgg cgacgacgaa ctgacaccgc acggcggacg cagcaccgtt gaggccttca
     1321 tgcggaaggc ggcggaggcg gtttgcgatt tttacataga gaatgccacg gcctccgatg
     1381 gcatacctta ctgggacacg ggagccccag ggctggcgaa cctgaaagac tggcgcagcc
     1441 gcccctccga cccattcaac gatcatgagc cggtcgatag ttccgccgcc gtcatcgcgg
     1501 cgcaggggct gttacggctg gggcggtatc tgaaaaatga ccggtattgg aacgctgggc
     1561 tcactgtgtg ccggacgttg ttcgcccaac cctacctcag caccgacccg aaccatcagg
     1621 gactcattct gcactctatt taccaccggc caaacggatg ggacaagatt ccggatggcc
     1681 gcaaaatccc gtcgggggaa gcgtgcatgt ggggcgacta ccacgctatg gaacttgccc
     1741 tctacgtgca gcgcatcctg accaacaagc cttactacaa attctggagc gaaactcagt
     1801 gatcattgcc gatctttccc aaattgccgg aagaacctat ccggcgcgcc gccgcacaca
     1861 gaacctggtc ggaggcgcga gccctattca agccaccaac ttctccattg ggcatgtgac
     1921 tctcgagccg gacggcggac aggtgccctg gcacaatcag gaacaggaag agatttattt
     1981 catcatcgaa ggcacggccg aaatgtgcct cggcgaagag cgccaaactc tttcgaccgg
     2041 acaggccgtc tatattccat ccaaagtttt ccatcagctc acgaataccg gcagcacgcc
     2101 ttgccggatg ctctactgct acggtccggc cggcgatgtc gcccactggc gccaggagct
     2161 cgaaggaaca cttcccaaag ccggcgtcga catgcccccg cttccagcgg gtgccaggcc
     2221 gcaatgcacc gaccggccga aagactaaac gaaaatgacc acacacaaaa tcggcattat
     2281 tatgaacggt gtcaccggac gcatggggct gaaccagcac ctgcgccggt ccatttacga
     2341 catcatgcag cagggcggcg tcaggacgcg ggacggcgtg atcatgccgg ctccgctact
     2401 ggtgggccgc aacgccgcaa agctccaagc catttcggcg gagtgcggcg gcctgccctg
     2461 gaccaccgac ctcgacagcg cgctcgccga cccgcagtac acggtttact tcgatgcaca
     2521 gactacgaac cggcgcgcgc cggacgtatc aaaggcgatc aaggccggaa aacatattta
     2581 ttgcgagaag ccgacctccg acagcctcga ggttgctctc gacctgtacc gccaggccaa
     2641 ggccgccggg gtgaagaacg gcgtggtgca ggacaagctg tggctgccgg gcctgctgaa
     2701 ggtcaagact ctggaccagc ttggcttttt cggcgacatt ctttcggtgc gcggcgaatt
     2761 cggctattgg gtctttgaag gagacaaggt gccggcgcag cggccgtcct ggaactatcg
     2821 caaggaagag ggtggcggaa tcatcatcga catgctttgt cactggcggt atgttctcga
     2881 caacctgttc ggagaagtga aatccgtttc ctgcctcggc gcaacccaca tcccaacgcg
     2941 atgggacgag gatgacaagc cttacgcctg tacggccgag gactccgtgt atgcgacgtt
     3001 tcagcttgcc agcggcgtga tcgcgcactt caattcctcc tgggtggtgc gtgtacgccg
     3061 cgatgacctc ctcacacttc agattgacgg tacgaaaggc acagccgttg ccggcctgcg
     3121 ggacgcctgg attcagcctt atgccgccac gccgcgtccg gtctggaatc cggatatccc
     3181 gcagccgctg agcttcttcg atggctggca gaaggttccg gagcagaccg gctatagcaa
     3241 cgcattcaaa gttcaatggg agctgttcct caagcacgtg gtcaccggcg agccgtttcc
     3301 atgggatctg cttgcgggag ccaaaggcgt gcagcttgcc gagaaaggca ttgaatcctg
     3361 gcataaacgc tgctgggtcg atttggatcc cctggaagcc tgagccgggt gctaatctga
     3421 tatagcattg cccatggagc aaccacagga ggccgatcaa ccccagcccg ctgcggttgc
     3481 cgcacgcgaa gattccacca tccggcttct acaggcgcgc gcaatacgcg tcgcctacgt
     3541 actggttttt ctgatcagtt tcggcgccgg gctcatcggg tggtacctga ttggtgggcc
     3601 cgggcatttt gaaataatgg gctggcacgt caagctggtg ctcagcgtgg cgtttgccgc
     3661 cgccgtcacg aaactggcac gcgcatccac actggaagac cgcgcatgga accggcggtc
     3721 tttgcgatgg ctgggatacg tcatcactct ggctgttttg atggcgacag cgacatatta
     3781 ctaccatctt caggagccga ttgaagaaga aatggttccg gaggagtcgg agtcgccgct
     3841 gtaagagcct cctcgtcgtg agttgaggct caagaaaaca ggttggcggg gtttgaaggc
     3901 aggtccctag aggttcttac ttagagttaa tactgaggtg tcggagagga taaccttatc
     3961 ttttcttgag ccttcctcaa cagcgtactc acgcccgcct agcatgcacg tctccaacca
     4021 attcagacaa tcaataacag caataactta catgaaactc tagggccgcc gggggtggca
     4081 ataattgcag attcggtact accgtaccaa aatgggacaa ccaccaacgt tatttggcca
     4141 aacggccctt ggtaaacgct tcgccatcac ggaggatgta tgcgacttta cggccagtaa
     4201 atgtcagcaa cgggcgttat acggccaagt ggtttttagt tggtacaagc cgacttctgg
     4261 ggagtaatct ggcgtccgga gtgccgggct acaccgtatg tctcaaatct gatacattta
     4321 gagggtgcgg ccggttatct tccatccgaa ggcgcgggat gagatccgcc ggtttcccaa
     4381 agaaccgcga gcccgtctcg ggcggggcct gtatcggttg cagatggggg aatcattgag
     4441 catgccgaac gcgcggccga tggcaactat agcttcgggc gtttgggaat tgcgcgtgag
     4501 aggtgaggat ggaattttcc gggtgttcta ttacacgaca tccggggccg gcgtcctggt
     4561 tttccatgcg ttcgcaaaga agacccagcg gactccggca tcggagatcg aactggcgag
     4621 aaaacgtttg aaggagcttc tcgat
//
LOCUS       DASTMK010000610        14761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_319790, whole genome shotgun sequence.
ACCESSION   DASTMK010000610 DASTMK010000000
VERSION     DASTMK010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14761
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319790"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3881)
                     /locus_tag="VFQ79_25150"
     CDS             complement(<1..3881)
                     /locus_tag="VFQ79_25150"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU0143033.1"
                     /translation="MQSSSPTRAEKSGLSPEKRALLELRLRGAAKGVVQDHSISRKPV
                     HDSAPLSFAQQRLWFLDQLAPGSSFYNVPAAVPLRMPLNASLLERSLNEIIRRHEVLR
                     TRFVTVDGQPVQVIAEALAVEVPLVDLRHLPEGERQAEATRLANQEARQPFDLTRGPL
                     LRARLLRLADSEYVFCLTVHHVVCDGWSMGLLFQELNQLYTAYALGRPSPLEELAVQY
                     ADYAMWQREALQGEVLEEQLEYWRKQLAGIVALELPSDHARPATPSYRGGQVEVRLPR
                     GLTGRLKALSQREGVTLFMTLLAAFQILLERYTGQEEVVVGSPIAGRNRAEIEPLIGF
                     FVNTLVMRTRLQGNPGFREALHRVQEVALGAYAHQDLPFEMLVEKLQPERDLSRNPLF
                     QVTFQLLNAPTVGSGVTAVRNEGGVEVTRGTSIFDLACNLVETGEEVWGGIEYSTDLF
                     ERWRIERMAEHYVALLEGIVEDPERRVRELPLLRERERRQMLVEWNATKAEVAGGSVI
                     EQFEAQVERTPEAVAVCCGGEQVGYGELNRRANQLGHRLRELGVGPEEVVGVCAERSV
                     EMVVGLLAVWKAGGAYVPLDPAYPEARLRYMREDAGIRILLTERSLAGRFAGERLELV
                     WLEDEGGTLPNLPHRNLPHNLAYVIYTSGSTGMPKGIMIPHRGLMNYLSWCTQAYDVE
                     AGSGAPVHSSISFDLTITGLFSPLLAGRNVELLPEGRVESLGTLLKSRSNFSLVKITP
                     AHLAMLSQELQPEEAVGKTRAFIIGGSNLLAEHIKFWQESAPETVLINEYGPTETVVG
                     CCVYRVPREKHSSGAIPIGRPIANTRLYVLDAHMQPVPVGVSGELYIGGDGVARGYLK
                     RPDLTAEKFVPDPFSDEPGHRLYRSGDLARYLPDGNLEYLGRMDQQVKIRGFRIEPSE
                     VETALKRHPGVLEACVVARDHELSEKRLMAYFVPAGETAPSVTELRTSLQETLPEYMI
                     PSVFLAVPELPLTANGKVDTRALPEPDAARPRIHTKYAPPRNEVEEKLVAIWKQVIGL
                     ERIGIHDNFFELGGDSILSIQVIARANQAGLHLTPQQLFLHQTISELAAVAGTSSAVE
                     AEQGDVTGPVPLTPIQKWFFEQELPEPHHYNQSVLIEVGPALNLKLLEMIAERLMAHH
                     DALRLRFKREGSEWRQAGTPSGGPAPVSRIDLSGVAAGEQMAALAAKAAGLQASLNLA
                     QGPIARFALFEFGGSRPNQLLILIHHLAVDGVSWRILLEDFEIAYMQLSRGESIALGR
                     KTTSIRQWAERLAERGRSSTLRDELEFWLHA"
     gene            complement(3883..8319)
                     /locus_tag="VFQ79_25155"
     CDS             complement(3883..8319)
                     /locus_tag="VFQ79_25155"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU0143034.1"
                     /translation="MAEGFVAPLSFAQQRLWFLDQLAPGSSFYNVPAAVPLRMPLNAT
                     LLERSLNEIIRRHEVLRTRFVTVDGQPVQVIAETLAVEVPLVDLRHLPEGERQAEATR
                     LANVEARQPFDLTRGPLLRARLLRLGDSEYVFCLTVHHVVCDGWSMGLLFQELNQLYT
                     AYALGRPSPLEELPVQYADYAMWQREALQGEVLEEQLEYWRKQLAGIVALELPADHAR
                     PATPSYRGGQVEVRLPRGLTGRLKSLSQREGVTLFMTLLAAFQILLERYTGQEEVVVG
                     SPIAGRNRAEIEPLIGFFVNTLVMRTRLEGNPGFREALHRVQEVALGAYAHQDLPFEM
                     LVEKLQPERDLSRNPLFQVTFQLLNAPTVGSGVTAVRNEGGVEVTRGTSIFDLACNLV
                     ETGEEVWGGIEYSTDLFERWRIERMAEHYVALLEGIVEDPERRVWELPLLREWERRQM
                     LVEWNATKAEVAASSAIEQFEAQVERTPEAVAVCCGGERVGYGELNRRANQLGHRLRE
                     LGVGPEEVVGVCAERSVEMVVGLLAVWKAGGAYVPLDPGYPEARLRHMREDAGIRILL
                     TQPSLAERFTEEGLELVLLEDDGGTLPNLPHQNSPHNLAYVIYTSGSTGTPKGVEIEH
                     AGLSNLICWHQRTYGVSQQDRATQIATPAFDAAVWEMWPYLTTGASIHLPDDSVRSSA
                     ELLAQWLVRERITICFIPTALAEAVVEEPWTEDCALRYLLTGGDRLRSSPRRALPFEI
                     VNHYGPTENTVVTTCAPVVRETENGSPPPIGRPIANSWIYVLDQHLNPVPAGIPGELY
                     IGGQGLARAYRGRPELTAAAFLPNPFTADPGARMYRTGDLVRYRPDGNVEFLGRLDHQ
                     VKVRGFRIEPGEIEAALKQHSAVENVAVVAAQDPSGGARLIAFVVPGRTDKTTDPQFE
                     HILRWQRIYDEIYSQPPPEADSTLNINGWISSYTGLPIPAGEMREWVESTTREILSWT
                     PNRVLDIGCGSGLLLFRIAPHCQRYIATDFSSAAIGYVQAQLDSQGLRNVELRHQFAD
                     DFQGMEQGAFDAVILNSVTQYFPSAGYLLQVLEGAVKAATPGGIVFVGDVRSHPLLEA
                     LHVSVETERSRGTLRLSELHHRVRQRIAQERELVLSPAFFTALGHRLPCISRVQIQPR
                     RGHSRNEMNCFRYNVVLHVDSAMQDPEPGVILDWRQDGFSREALDRLFAERHAQALIT
                     NVPNQRVEPAFSLLDLLELMKDSDGTATVAELRDAAREVSSTGIDPEEIYAAGERAGY
                     QVALRISESAGCFDALLTQRSEAGRTPLLFARQQYSAPAGEDWSHYANNPVEASVERD
                     LPAVLRAHSKEHLPEYMIPSAFVPLDALPLTSNGKVDRQALLALDVRQEALQDCAPPR
                     TEVEAALAGLWAEVLGVDRVGIQHNFFTELGGHSLLATQLLSRIRSAFQVELPLRRLF
                     EAPTVATLALVLEEALVAQVEQMSDEEARRLLQASEPQPVRS"
     gene            complement(8347..9789)
                     /locus_tag="VFQ79_25160"
     CDS             complement(8347..9789)
                     /locus_tag="VFQ79_25160"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU0143035.1"
                     /translation="MDGRPAARLFLNSDIGDAPISFENYIEYLAGEIVPYSINLSSPR
                     CLGHMTGVVPGFIRPLADLVTRLNQNMVKREASGIFSALERQTIAMMHRTVFGLPESF
                     YNDHIQNQLSTLGMMTSGGTLANLTGLWIARNTSFAPSGPFTGIENEGLPAALRHYGS
                     RDAVIVGSNLVHYSIDKAAGILGLGVKNLIKVPIDRSNGIDLDALRRCVRNCADEGRK
                     IIAIIGIAGTTDCGSIDPLDEMASIAREAGVHFHVDGAWCLPLLFSRRHCSKLAGVEK
                     ADTVTFDGHKQLCLPSGSGMLLIRDPHAARVIEKQTRYILQEFSGDLGKHSIEGSRPG
                     TALFLHAALRLIGRNGYEALIDESICRAQFMADLIRHRPDFQLLTEPETNIVLYRYIP
                     ELFRSTCPRELTPADNQSIDTYNERIQQAQFRSGRSFVSRTQWDYVSEEEPVTVVALR
                     AVVANPFTSQEDIRFVLEDQARIAQELAAG"
     gene            10145..10621
                     /locus_tag="VFQ79_25165"
     CDS             10145..10621
                     /locus_tag="VFQ79_25165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143036.1"
                     /translation="MEKAKSAESSTKGVDLQSRSTAIYMDLVRALQDAGMTSQKYVDD
                     SARTYLAKLREVSDDVQRRYREAYLTYALATQQALAGVSQQPQALDAQRDFVAAAQNI
                     ESDLQKRLEQANREWLDAVQSGQTDVKNRLREAYRNYLRGQQELWASLDINALVGA"
     gene            complement(10631..11539)
                     /locus_tag="VFQ79_25170"
     CDS             complement(10631..11539)
                     /locus_tag="VFQ79_25170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143037.1"
                     /translation="MNILSAGAEATPKPRVLAQAPEELTNGRLQRLGEGIGKVVYASE
                     HWVVKRKRSPSEIVALIALWKLLRKFERWLPFGLGVKWLERPSRQIRFLRIMLQGIVV
                     IIPRSVWFTSHIGEVWRVYRKRDLRGEKLARIHLAGTPLAPVRITFPPARIKIGAWPG
                     WLMASEAIERVDETLYHRLRTLAGAGRFAELERWLHRFLDTRQAGWEHGIFSLDAHLK
                     NFGVCGDRVVLLDTGGLTDDWSEIEQHLALEDVVTEPHIQLGLGPLLGSRPDIADRFN
                     KRWKAAVNPSAVRERWPTGPDNGRGL"
     gene            11685..12800
                     /locus_tag="VFQ79_25175"
     CDS             11685..12800
                     /locus_tag="VFQ79_25175"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEU0143038.1"
                     /translation="MKQDFSRRRMMQAVSAAALPGVTMSAAAVARSWPVTEGPDTPKI
                     CVGLGDGRSGSAQAGEASLRRVKQLGVNYVLGGGPRIPWQESELRERMEQLKAGGIAL
                     YNFMISGFPNTLYGRPGRDEEIEKVQQSIRAAGRVGLPVVEYNFYAHRLVEGYYEETG
                     RAGAGLTAFDYDRVKNLPPLAEIGAWDLEQMWSNITYFLKAIEPVAEEAGVLLALHPN
                     DPPAPLSRGSGQIMGTLAGWKRLIDIVPSNANGITYDCGVTRELGEDPVEVCRYFGSR
                     KRINHVHFRNVRVRKPYESYTEVFPDEGQVDMFGVMKELVRQKYTGVIYPEHPRALDA
                     DRERPNFKPYYPGGGGYTGIAYNVAYTRAMLQAALSS"
     gene            complement(12959..13354)
                     /locus_tag="VFQ79_25180"
     CDS             complement(12959..13354)
                     /locus_tag="VFQ79_25180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143039.1"
                     /translation="MTLHPDILTDLIVLYHAGEASQASRALLEEEAARNPQIAAALAA
                     GPRPVPTLPSGPAPDERKVLRKVRLRYQAISFGIIWMLALLAVVLLPRFVNTRTGIEI
                     AISLMPFAVLILFFAGAVAALYFFIRAVR"
     gene            complement(13351..13797)
                     /locus_tag="VFQ79_25185"
     CDS             complement(13351..13797)
                     /locus_tag="VFQ79_25185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0143040.1"
                     /translation="MSEIYTEHAAAVYRCLLGWTRSPALAEELTAETFYRAIVADQQV
                     RAVTARGYLIAIARNEWRKLCAKRNREQPLVADATAPGQSGEALVDFQLMLAALSDLP
                     DELREPLVLYAQGGLSYEEIAVQLNITLATVKIRIYRARQKLEKYR"
     gene            complement(13913..>14761)
                     /gene="moaA"
                     /locus_tag="VFQ79_25190"
     CDS             complement(13913..>14761)
                     /gene="moaA"
                     /locus_tag="VFQ79_25190"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU0143041.1"
                     /translation="EIERFVRIAARLGITKLRVTGGEPLVRKDLPVLIRMLRSIEGIC
                     DIALTTNGVLLASQAEELYEAGLRRINVHLDTLDRQRFIEIARRDELDKVLEGIEVCR
                     RMGYGPIKINAVAVKNLVEPDIVPLARFGREHDIEVRYIEFMPLDARGLWRRDKVLLA
                     DDIIRLLTEGIGPLVEVPSRDPSAPALEYRFADGIGRIGFIASVSRPFCLNCNRIRLT
                     ADGKLRYCLFAIDETDIKTLLRSGVPDEEIARSIRSTVADKWEGHEINSSRFVAPPRP
                     MYSIGG"
BASE COUNT         2913 a         4841 c         4214 g         2793 t
ORIGIN      
        1 tgtgcgtgga gccagaactc cagctcatcg cgcagcgtgg acgagcggcc gcgttccgcc
       61 agtctctccg cccactgccg gatagatgtc gtcttgcggc cgagcgcgat gctttctcca
      121 cggctgagct gcatgtacgc gatctcaaaa tcctcaagca ggatacgcca ggagactcca
      181 tccacggcca ggtgatggat gaggatcagt agctgattcg gcctcgaacc gccgaactcg
      241 aacagcgcga acctcgcgat cggtccctgt gccagattca gactcgcttg caaccctgcg
      301 gccttcgctg ccagcgccgc catctgctcg ccggcggcga ctcccgacag atcgatccgg
      361 ctgacgggtg ccggtccacc cgacggcgtg ccggcctggc gccactcgga accttctctc
      421 ttgaagcgca ggcgcagagc atcgtggtgg gccatcaacc gctccgcaat catttccagc
      481 agcttgagat tcaatgcggg cccgacctcg atcaacacgg actggttgta gtggtggggc
      541 tccggcagct cctgctcgaa gaaccatttc tgaatcggcg tcaaaggcac gggaccggtg
      601 acatcgccct gctcggcttc caccgcggag gaagtgccgg caacggccgc cagttcggat
      661 atcgtctggt gcaggaacaa ctgctgtggt gtaaggtgca gacccgcctg gttcgcacgg
      721 gctatgactt gaatactcag aatcgaatcc ccgcccagct cgaagaagtt gtcgtgaatg
      781 ccgatgcgtt cgaggccgat cacctgcttc cagatcgcaa cgagcttttc ttccacttcg
      841 ttccgcggcg gtgcatactt cgtgtgaatc cgcggccgcg ccgcatccgg ctccggcagc
      901 gcccgcgtgt ccacctttcc gtttgcggtg agcggaagtt cggggacggc gaggaaaaca
      961 gacggaatca tgtactccgg cagcgtttcc tgcagtgagg ttcgcagttc ggtcaccgaa
     1021 ggagcggtct cgccggcggg cacgaagtag gccatcaggc gcttctccga gagttcgtga
     1081 tcgcgcgcga cgacacaggc ctccagaaca ccggggtgcc gctttaacgc tgtttccacc
     1141 tcgctgggct caatacggaa gccgcggatc ttcacctgct ggtccatccg cccgagatat
     1201 tccaggttgc cgtctgggag gtatcgcgca aggtctccac tccggtagag gcggtgtccg
     1261 ggctcatcgc tgaaaggatc gggcacgaat ttctccgcgg tcaaatcagg ccgcttcaag
     1321 tatcctcgcg ccacgccgtc gccgccgatg tacaactctc ccgaaacgcc caccggcacc
     1381 ggctgcatgt gcgcgtccaa cacgtaaagc cgcgtgttcg cgatgggccg gccgattgga
     1441 atcgctccgg acgagtgttt ttcccggggc acgcggtaga cgcagcagcc cactaccgtt
     1501 tccgtgggac cgtattcgtt gatcagaacc gtttccggag ccgattcctg ccagaacttt
     1561 atatgctccg ccagcagatt cgatccgcca ataatgaatg cccgggtctt gcccacggct
     1621 tcctccggct gcagttcctg gctgagcatt gccagatgcg ccggtgtgat cttgaccagg
     1681 ctgaaattcg aacggctttt cagcaatgtg ccgagacttt cgacgcggcc ttcggggagc
     1741 aactcgacgt tccggcccgc cagcaacggg gaaaacagtc cggtgatcgt caggtcgaaa
     1801 gaaatcgacg aatgcacggg tgcgccgctg cccgcttcca cgtcgtacgc ttgtgtgcac
     1861 caggacaaat aattcatcaa gccccggtgc gggatcataa tccccttggg cataccggta
     1921 gaaccggagg tgtagatgac gtacgccagg ttgtgaggca agttccggtg gggaagattc
     1981 ggaagcgtcc cgccttcgtc ctccagccat accagttcga gccgttcccc ggcaaaacgc
     2041 cccgccaggc tccgctcggt gagcagaatc cggatcccgg catcttccct catgtaccgc
     2101 agccgcgctt ccggataggc cgggtccagc ggtacatacg ctcctcccgc tttccacacc
     2161 gccagcagcc ccaccaccat ctccaccgaa cgttccgcgc agacccccac cacttcctcc
     2221 ggccccaccc ccagctcccg cagccggtgc cccaactggt tcgcccgccg gttcaactcc
     2281 ccgtagccaa cctgctcccc gccgcagcac accgccaccg cctccggcgt ccgttccacc
     2341 tgcgcttcga actgctcaat aacactccct cccgctacct ccgccttcgt cgcgttccac
     2401 tccaccaaca tctgccgccg ttcccgctcc cgcagcagcg gtaactcccg cacccgtctc
     2461 tccggatcct ctacgatccc ttccagcagc gccacatagt gctccgccat ccgttcgatc
     2521 cgccatcgct caaacaggtc cgtgctgtac tcgattcctc cccacacctc ttctcccgtc
     2581 tccaccaggt tgcacgccag atcgaagatc gacgtccccc gcgtcacctc cacgccccct
     2641 tcgttccgca ccgccgtcac cccgctgccc accgtcggcg cgttcagcaa ctggaatgtc
     2701 acctgaaata acgggttccg gctcaggtcc cgctccggct gcaacttctc caccagcatc
     2761 tcgaacggca gatcctggtg cgcatacgcc cccagcgcca cctcctgcac ccggtgcagc
     2821 gcttcccgga aaccaggatt cccttgcagc cgtgtccgca tcaccagcgt gttgacgaaa
     2881 aacccgatca gcggctcgat ctccgcccgg ttgcgcccgg cgatcggcga gcccaccacc
     2941 acttcctcct gtcccgtgta gcgttcgagc agaatctgaa aggcggccag cagcgtcatg
     3001 aacagcgtca ccccttcccg ctggctcagc gccttcaacc tgcccgtcag cccccgcggc
     3061 agccgcacct ccacctgccc gccccggtag cttggtgtag ccggacgcgc gtggtccgac
     3121 ggcagctcca gtgccacgat ccccgccaac tgcttccgcc agtactccaa ctgctcttcc
     3181 agcacctccc cttggagcgc ttcccgctgc cacatcgcgt agtcggcata ctgcaccgcc
     3241 agctcttcga gcggtgaggg ccgacccaac gcataggccg tgtacaactg gttcagctcc
     3301 tggaacagca gccccatcga ccagccgtca cacaccacgt ggtgcaccgt caggcagaac
     3361 acgtactcgc tgtcggccag acgcagcaga cgcgcccgca gcaggggacc tcgcgtcagg
     3421 tcgaacggct gccgcgcctc ctggttggcc agcctagtag cttccgcctg ccgctccccc
     3481 tccggaagat gccgcagatc caccagcggc acttccaccg ccagcgcttc ggcgatcacc
     3541 tgtacgggtt gcccgtccac cgtcacgaaa cgggttctca atacctcgtg gcggcggatg
     3601 atctcgttca ggctgcgctc cagcaggctg gcattcagcg gcatgcgcag cggtactgct
     3661 gccggtacgt tataaaaaga actccccgga gccaactggt ccagaaacca cagccgctgc
     3721 tgcgcgaacg ataggggagc ggaatcgtga accggtttcc gcgaaatgct gtgatcctgc
     3781 acaacccctt tcgcggctcc acgcagtctc aactcgagaa gcgcgcgctt ttccggcgat
     3841 agcccggact tttctgcacg ggttggagag ctggattgca tttcagctcc tcaccggctg
     3901 cggctcgctg gcctgcaaca gccgacgcgc ctcctcgtcg ctcatttgtt ccacttgcgc
     3961 tacaagcgcc tcttccagca ccaaggctaa cgtggccacg gtgggagctt caaacagccg
     4021 gcgcagaggc aactccacct ggaatgcact gcgaatgcgg gagagcagtt gcgttgcaag
     4081 aagcgagtgc ccacctagtt ctgtaaaaaa gttgtgttga attcctactc tgtccacgcc
     4141 caggacctcc gcccagagac ccgctagcgc ggcttcgacc tcggtgcgcg gcggcgcgca
     4201 atcctgcaat gcctcctgcc ggacatccag agcgagcaac gcctgccggt ccactttccc
     4261 gtttgacgtc agcggcagcg cgtcgagcgg cacaaacgcc gatggaatca tgtattccgg
     4321 caggtgctcc ttcgagtgag cacgcaacac ggctggcagg tcgcgctcta ccgaggcctc
     4381 tacgggattg ttggcgtagt gggaccaatc ctcgccggcg ggagcgctat actgctgccg
     4441 tgcgaaaagc agtggtgtcc gtccggcttc gctgcgctgc gtaagaagag catcgaagca
     4501 gcccgcgctt tccgaaatac gaagcgcaac ctgatagccg gcccgttcac cggccgcata
     4561 gatttcttcg ggatcgatgc ctgtgctgct cacctcgcgg gcggcatcac gcaactccgc
     4621 gaccgtcgcc gttccgtccg agtctttcat caattccagc aggtccaaca gactgaatgc
     4681 aggttccacg cgctggttgg gcacattcgt aatcaaagcc tgcgcgtgcc tttccgcgaa
     4741 cagccggtcg agcgcttccc tggagaagcc atcttgccgc caatccagaa tcacgccggg
     4801 ttcgggatct tgcatcgccg aatcgacgtg aagaaccacg ttgtagcgga aacagttcat
     4861 ctcgttgcga gagtggccgc gccggggctg aatctggacg cggctgatac acggcagccg
     4921 gtgcccgaga gccgtgaaaa acgctggaga cagaaccaac tcccgctcct gcgcgatgcg
     4981 ctgccggaca cgatgatgga gttcgctcaa ccggagagtg ccacgagagc gctccgtttc
     5041 tacggaaaca tgaagcgcct cgagaagagg atggctccgc acgtctccca cgaagacaat
     5101 tccgccgggc gtagccgcct ttacggcgcc ttccagcacc tgaaggagat acccggcgga
     5161 cgggaaatat tgtgtgaccg agttgaggat gacggcgtca aaggcgcctt gttccatccc
     5221 ttggaagtcg tccgcgaact ggtgacgcaa ttcgacattg cgcaggccct ggctgtccaa
     5281 ctgcgcttgc acatacccga tcgccgcact cgaaaaatcc gtagcgatgt agcgctggca
     5341 gtgcggcgcg atccggaaaa gtagcagccc gctgccgcag ccgatatcca acacccggtt
     5401 gggagtccat gacaagatct cgcgggttgt actctcgacc cattcccgca tttctccggc
     5461 ggggatcggc agccccgtgt aactgctgat ccagccattg atgtttaacg tggagtccgc
     5521 ttccggtggc ggctgtgagt agatctcgtc gtaaatccgc tgccatcgca agatgtgttc
     5581 gaactgcgga tcggtggtct tatctgtccg gcccggcaca acaaaggcaa tcaaccgggc
     5641 gccgcccgat ggatcttgcg ccgctacgac ggcaacgttc tctacggcgg agtgctgctt
     5701 taacgccgcc tcgatctcgc caggctcgat gcggaatccc cgcactttaa cctgatgatc
     5761 cagacgcccc agaaactcca catttccatc aggccggtat cggaccaggt cccctgtccg
     5821 gtacatgcgg gccccgggat ccgccgtgaa cggattcgga agaaatgcgg cggccgtcaa
     5881 ctcagggcgg ccacggtatg cccgcgccag cccctgcccg ccgatgtaaa gctcgcccgg
     5941 gatgccggcg ggaacgggat tgagatgctg atccagtacg tagatccagg agtttgcgat
     6001 aggacggccg atgggcgggg gcgaaccgtt ttccgtctct cgaacgaccg gtgcacacgt
     6061 tgtcaccacg gtgttctcgg tgggtccgta gtggttgacg atttcaaacg gaagcgcgcg
     6121 cctgggagaa ctcctcagcc tgtcgcctcc ggtcaacaga tagcgcagag cgcaatcttc
     6181 cgtccacggt tcctcgacaa cggcttcggc aagcgcggtg gggatgaagc agatcgtgat
     6241 ccgctcgcgg acgagccact gcgccagcaa ctcggccgag cttcttaccg aatcgtcggg
     6301 gagatggatc gaggcgccgg tggtgagata cggccacatc tcccagacgg ccgcatcgaa
     6361 ggctggtgtc gcgatctggg tcgcccggtc ctgctggctt accccgtacg tacgttggtg
     6421 ccagcaaatc aggttcgata atccggcatg ttcaatttcg acgcccttgg gagttccggt
     6481 agagccggaa gtgtagatga cgtacgccag gttgtgaggc gagttctggt ggggaagatt
     6541 cggaagcgtc cccccgtcgt cctccagcaa aaccagttcg agcccctcct cagtgaaacg
     6601 ttccgccaag ctcggctgcg tgagcagaat ccggatcccg gcatcttccc tcatgtgccg
     6661 cagccgcgct tccggatagc ccgggtccag cggcacatac gcccctcccg ctttccacac
     6721 cgccagcagc cccaccacca tctccaccga acgttccgcg cagactccaa ccacttcctc
     6781 cggccccacc cccaactccc gcagccggtg ccccaactgg ttcgcccgcc ggttcaactc
     6841 cccgtagcca acccgctccc cgccgcagca caccgccacc gcctccggcg tccgttccac
     6901 ctgcgcttcg aactgctcta tggcactgct tgccgctacc tccgccttcg tcgcgttcca
     6961 ctccaccagc atctgccgcc gttcccactc ccgcagcagc ggcaactccc acacccgtct
     7021 ctccggatcc tccacgatcc cttccagcag cgccacatag tgctccgcca tccgctcgat
     7081 ccgccagcgc tcaaacaggt ccgtgctgta ctcgattcct ccccagacct cctcacccgt
     7141 ctccaccagg ttgcacgcca gatcgaagat cgacgtcccc cgcgtcacct ccacgccccc
     7201 ttcgttccgc accgccgtca ccccactgcc caccgtcggc gcgttcagca actggaatgt
     7261 cacctgaaat agcggattcc ggctcagatc ccgctccggc tgcaacttct ccaccagcat
     7321 ctcgaacggc aggtcctggt gtgcatacgc ccccagtgcc acctcctgca cccggtgcag
     7381 cgcttcccgg aaaccaggat tcccctccag ccgcgtccgc atcaccagcg tgttgacgaa
     7441 aaacccgatc agcggttcga tctccgcccg gttgcgtccg gcaatcggcg agcccaccac
     7501 cacttcctcc tgtcccgtgt agcgttcgag cagaatctga aaggcggcca gcagcgtcat
     7561 gaacagcgtc accccttccc gctggctcag cgacttcaac ctgcccgtca gcccccgcgg
     7621 cagccgcacc tccacctgcc cgccccggta gcttggcgta gccggacgcg cgtggtccgc
     7681 tggtaactcc agtgccacga tccccgccaa ctgcttccgc cagtactcca actgctcttc
     7741 cagcacctcc ccttgcagcg cttcccgctg ccacatcgcg tagtcggcat actgcaccgg
     7801 cagctcttcg agcggcgagg gccgacccaa cgcatacgcc gtgtacaact ggttcagctc
     7861 ctgaaacagc agccccatcg accagccgtc gcacaccacg tggtgcaccg tcaggcagaa
     7921 cacgtactcg ctgtcgccca gccgcagcag acgcgcccgc agcagcggac ctcgcgtcag
     7981 gtcgaacggc tgccgcgctt ccacgttggc cagcctggta gcttccgcct gccgctcccc
     8041 ttccggaaga tgccgcagat ccaccagcgg cacttccacc gccagcgtct cggcgatcac
     8101 ctgtacgggt tgcccgtcca ccgtcacgaa acgggttctc aatacctcgt ggcggcggat
     8161 gatctcgttc aggctgcgct ccagcagggt ggcattcagc ggcatccgca gcggcactgc
     8221 cgccggcacg ttataaaaag aactccccgg cgccaactgg tccagaaacc acagccgttg
     8281 ctgcgcgaac gataggggag ccacaaaccc ttctgccaat acgcacctcc ttaattgatt
     8341 ccggctctaa ccggcagcca gttcctgtgc gatccgcgcc tggtcctcca gaacaaaccg
     8401 gatatcctcc tggctggtga aagggtttgc aacgacggct cgcagcgcga ctaccgtcac
     8461 aggctcctct tcggaaacgt agtcccactg ggttcgggac acgaaagagc ggcccgaccg
     8521 gaactgggcc tgttgtatcc gctcgttgta cgtgtcgatc gactggttat ccgcgggcgt
     8581 caactcccgc gggcaggtgg agcggaacag ttccggaatg taccggtaga ggacgatgtt
     8641 tgtttcaggc tcggtcagca actggaagtc cggacggtgc cggatcagat ccgccatgaa
     8701 ctgcgcccgg cagatacttt cgtcaatcag agcctcatag ccattacgcc cgatgaggcg
     8761 aagcgcagcg tgcaggaaca acgccgttcc cggacgcgat ccttcgatgg aatgcttgcc
     8821 aaggtctccg gagaactcct gcaggatata gcgtgtctgt ttctcgataa cccgcgcggc
     8881 gtgcggatcg cggatcaata gcatcccgct gccgctggga aggcacaact gtttgtggcc
     8941 gtcaaaagtg actgtgtccg ccttctcgac ccccgccagt tttgagcaat ggcggcgtga
     9001 aaacaaaagc ggtaagcacc aggcgccgtc cacgtgaaag tggacgccgg cttcccgcgc
     9061 gatcgacgcc atttcgtcca gggggtcgat gctgccgcag tcggtggtgc ccgcgatgcc
     9121 tataatggca ataattttgc gcccctcgtc cgcgcaattc cggacacatc ggcgcagcgc
     9181 atcgaggtca atgccgttgg agcggtctat cggcactttg attaaattct taacgcccaa
     9241 acccagaatt ccggccgctt tatcgatgga gtaatgaaca agattggatc caacgatgac
     9301 cgcgtcgcgc gacccatagt ggcgcagcgc ggccggcagg ccctcattct cgatgccggt
     9361 aaaaggaccg gaaggagcga aggaagtatt tcgcgcaatc cacagaccgg tgagattggc
     9421 cagcgtgcct cccgaagtca tcattcccag ggtgcttaac tggttctgaa tgtgatcgtt
     9481 atagaagctt tccggaagtc cgaacacggt tctgtgcatc atcgcgatgg tctggcgttc
     9541 caacgcgctg aatattccgg aggcttcgcg cttcaccatg ttctggttca accgtgtgac
     9601 caggtcggcg agcggacgaa taaagcccgg gacaacgccg gtcatgtggc ccagacatcg
     9661 cggagaggaa agattgatgg aatacggaac gatttcgccg gccaggtatt cgatgtagtt
     9721 ttcaaacgaa atcggggcgt cgccgatgtc gctgtttaga aagaggcgcg cggccggccg
     9781 gccgtccatt tctccgtgaa cacggccctc attgcagata tccaaaaaag aatgaagcaa
     9841 acgcgcggat agtgtttccc ggtggtgatc cttaccatcg gaggaaaaca acgcagaaaa
     9901 accatcgcgg accgcatgat tgacagtcat cggagtctca ctgagacaat accggggaat
     9961 gcttaatgcg cctgtctggt gccggcgcta ttagcgttaa tgttccaatg catgcgctgc
    10021 cggcccttca aaattcaact tagtaatgca gttgataaaa cttaataaca gttacccagc
    10081 tttccgttga gctttcttga gaaagatgat aaaactgaat ccacccgttg gcaaggagga
    10141 aatcatggaa aaggcgaaat ccgccgagag cagtactaaa ggcgtggatc tgcaaagtcg
    10201 ttcgacggcg atatacatgg atctggttcg cgccctccag gatgccggaa tgacgagcca
    10261 aaaatacgtg gacgactccg cacgcacata cctcgcaaaa ctgcgggaag tatccgacga
    10321 tgtgcaacgg cgctaccgcg aagcttacct gacctatgcg ctcgctactc aacaggcttt
    10381 ggccggcgta tcacagcaac cgcaggcgct cgacgcccag cgcgatttcg tagccgccgc
    10441 gcagaacatc gagagcgatc ttcagaaacg cctcgaacag gcgaatcgcg aatggttgga
    10501 tgcggtgcag agcggacaga cggacgtgaa gaaccgcctc cgggaggcgt atcgtaatta
    10561 cctgcgcggt cagcaggaac tctgggcgtc cctggacatt aacgcactgg taggcgcata
    10621 gagccggaac ttacaacccg cggccgttgt ccggaccggt gggccagcgt tcgcggacag
    10681 ccgagggatt aaccgctgct ttccagcgtt tgttgaatcg atcggcgatg tccggccggg
    10741 agcccagcag cgggcccaga ccgagttgga tatgcggctc tgtaacgaca tcctccagtg
    10801 ccaggtgctg ttcgatttca ctccaatcgt cggttaagcc gccggtatca agcaggacca
    10861 cgcggtcacc gcatacgccg aaattcttga gatgcgcgtc aagggaaaaa atgccgtgct
    10921 cccatcctgc ctgccgcgta tccagaaacc gatggagcca ccgctcgagc tcggcgaacc
    10981 gccccgcccc ggcgagggtg cgaagccggt ggtagagtgt ctcatcgaca cgttcgatcg
    11041 cctcggatgc catcaaccaa ccgggccatg ccccgatctt gatacgtgcg ggcgggaacg
    11101 ttatccgcac cggcgccaga ggggttccgg caagatggat acgcgccagt ttctctcctc
    11161 gcaggtcgcg tttgcggtag actctccaca cttcaccgat atggctggtg aaccagacgc
    11221 tgcgcggaat gataacgacg atcccctgga gcatgatccg aagaaagcgt atttgtctgg
    11281 agggcctttc aagccatttg acgccgagcc cgaaaggcag ccagcgctcg aatttccgga
    11341 gcagcttcca tagcgcgatg agcgccacga tttccgacgg cgagcgcttt cgtttcacta
    11401 cccaatgctc ggaagcgtag acgactttgc cgattccttc gcccaggcgc tgtaaccgcc
    11461 cgtttgtaag ctcttccgga gcctgcgcga gcacacgcgg tttcggcgtt gcttcagcac
    11521 cggccgacag gatgttcata cagccactta tccacattat gattatgtcc ggctggacaa
    11581 cgcgtgaaca gtggcttgcg gttttgaatc gaccggtgct ataaccgcga gtgcctggag
    11641 gcggccggca gacgcgagcg gccactcggg caggagacaa gcccatgaag caggattttt
    11701 ccagacgacg aatgatgcag gcggtgagcg cggccgcgct tccgggcgtg acgatgtccg
    11761 cggcagccgt agcgcgatcg tggccggtca ccgaaggccc cgatacaccg aagatctgtg
    11821 tggggctggg cgacgggcgg tccggctccg ctcaggccgg ggaagcctcc ctgcgccggg
    11881 tgaagcagct cggcgtgaac tatgtgctcg ggggaggacc tcgcatcccg tggcaggaaa
    11941 gcgaattgcg cgagcgcatg gagcagttga aggccggcgg aatcgcactt tacaatttca
    12001 tgatttcggg ctttccgaac acgctatatg gcaggccggg ccgggatgag gagattgaga
    12061 aagtgcagca atccatccgc gcggcggggc gtgtggggct gcctgtcgta gagtacaact
    12121 tctacgcgca ccggcttgtc gaaggctatt acgaagagac aggaagggcg ggcgcgggtt
    12181 tgaccgcgtt cgactatgac cgcgtcaaaa acctgccgcc gctggccgaa attggcgcct
    12241 gggacctgga gcagatgtgg agcaacatca cctattttct gaaggcgatt gagccggtcg
    12301 ccgaggaggc gggcgtgctc ctggcgctgc atcccaacga tccgcccgcg ccgctcagcc
    12361 gcggttcggg ccagatcatg ggcacgctgg ccgggtggaa acggctcatc gatattgtcc
    12421 cgagcaacgc gaatggaatc acctacgact gtggagttac cagggagcta ggtgaagatc
    12481 ccgtggaagt gtgccgctat ttcggcagcc ggaagcgcat caatcacgtg cactttcgca
    12541 acgtgcgcgt gcgcaaaccg tacgaaagct acaccgaggt ttttcccgac gaagggcagg
    12601 tcgatatgtt cggcgtgatg aaggaactgg tgcggcaaaa gtatacgggt gtgatctatc
    12661 cggagcatcc gcgcgcgctc gatgccgatc gcgagcgacc caatttcaag ccgtactatc
    12721 cgggtggggg cggatatacc ggaatcgcgt acaacgtggc atacacgcgg gccatgttgc
    12781 aggcagcact cagctcttaa gccactccgc gcgggaaaag ggatgtgcgc cagatgctca
    12841 tgtgcgctgg acccgcttac gttgcttgcg gcccgcggga ttccggccat cgcgtcgacc
    12901 cgatgacagc gttgcgggcc gagtagtccc tttggcatcg gtgagtagaa ccggatgatt
    12961 agcggacggc gcgaatgaag aagtacagcg cagctacggc accggcaaaa aaaaggatca
    13021 gaaccgcaaa aggcatcagc gaaatcgcta tttctatgcc cgttctggtg tttacaaagc
    13081 ggggcagcaa cacgacagca agtaacgcca gcatccagat gatgccaaag gaaatcgcct
    13141 gatagcgtaa ccgaaccttg cgcagtacct tgcgttcgtc tggtgctgga ccggacggca
    13201 gcgtaggtac cggtcgtggc ccggcggcga gcgccgccgc gatttgcgga ttccgtgcgg
    13261 cttcttcctc cagcagagcg cgtgatgctt gggaggcttc gccagcgtga tagagaacga
    13321 tcagatcggt caggatgtca ggatgcagtg tcatctatac ttctccaatt tctgccgcgc
    13381 ccggtagatc cgaatcttca ccgtcgcgag tgtgatgttc agttgcaccg cgatctcttc
    13441 atagctgaga ccaccctgtg cgtatagtac gagcggttcc cgcaactcat cgggaagatc
    13501 tgagagtgcc gcgagcataa gctgaaagtc cacaagcgcc tcgccggatt ggccaggtgc
    13561 ggtggcgtcg gcaaccaggg gttgttcgcg attgcgcttc gcgcataatt ttcgccactc
    13621 gttgcgtgca atagcgatca ggtaaccccg agccgttact gctcggacct gctggtcagc
    13681 aacgatggcg cggtagaagg tctcggccgt caattcttcg gctaaggccg ggcttcgagt
    13741 ccatccgagc aggcagcgat acaccgcagc agcgtgctcg gtatagatct cgctcatcgt
    13801 catcgcgtat cttaatatcc gccagtttgc gatttgttac gaaacgaggg aacaaaatta
    13861 ccaaacggaa tgttgttcgt caggcttgag gagtggcggc tttcacttcg tttcagccgc
    13921 caatggagta catcgggcgg ggcggagcca caaagcggct actgttgatc tcgtgccctt
    13981 cccacttgtc tgccacggtc gaacggatgg agcgggcaat ctcttcgtcc ggcacgccgc
    14041 tgcgcaacag cgtcttgatg tccgtctcgt cgatagcgaa caggcagtag cgcagcttac
    14101 cgtccgcggt aagccggatg cggttgcaat tgaggcagaa aggccggctc accgaggcta
    14161 tgaagccgat cctcccgata ccgtccgcaa accggtattc gagcgcgggc gcactcgggt
    14221 cgcggctggg cacctcgacc aacggtccga taccctccgt aagcaatcgg ataatatcgt
    14281 cagccagaag caccttgtcc cgccgccaca gcccccgcgc atccagcggc atgaattcga
    14341 tgtaccggac ttcgatatcg tgctcccggc cgaaccgggc gagcggcacg atgtccggct
    14401 ctaccagatt cttcacggct actgcattga tcttgatcgg accgtagccc atccggcggc
    14461 aaacctcgat gccctcgaga actttgtcca gctcatcgcg gcgcgcgatc tcgataaagc
    14521 gctgcctgtc caacgtgtcg agatgaacgt taatgcggcg taaccccgcc tcatacaact
    14581 cctccgcctg cgaggcgagc agtacgccat tcgtcgtaag agcgatatcg caaatcccct
    14641 cgatggagcg gagcatgcgg atgagcacgg gcaggtcttt gcgcaccagc ggttcgccgc
    14701 ctgtcacccg gagtttcgtt attccgagcc tcgcggcaat tcgcacaaac cgctcaatct
    14761 c
//
LOCUS       DASTMK010000611         5205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_255290, whole genome shotgun sequence.
ACCESSION   DASTMK010000611 DASTMK010000000
VERSION     DASTMK010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255290"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFQ79_25195"
     CDS             <1..181
                     /locus_tag="VFQ79_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU0143042.1"
                     /translation="ATVELTVVETEPALKGATVSNVTKPAKLETGLVVQVPPFITEGE
                     KIRVSTAEGTYQERA"
     gene            complement(166..1491)
                     /gene="selA"
                     /locus_tag="VFQ79_25200"
     CDS             complement(166..1491)
                     /gene="selA"
                     /locus_tag="VFQ79_25200"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU0143043.1"
                     /translation="MTGRDELRNLPSVDELVSGVAADFENIPHKVLVDEARRLLASLR
                     TAMKTGEAAGVGSIGTQVQRAVKAILQPSLRLVINATGVILHTNLGRAPVPLDNSLQG
                     YSNLEYDLETGRRGKRDVHVTALLERLLGHGGIVVNNNAAAVYLVLNELAAGSEVIVS
                     RGELIEIGDGFRIPDIMARSGAILREVGTTNRTRLEDYRAAINERTRLLMRVHRSNFR
                     IEGFTASPSLNELARLAKERGLPVYEDLGSGCLVDLSPYGIDEPLARASLDAGASLVS
                     FSGDKLLGGPQAGIIAGRAELVQRIRRNPMFRALRVDKLTYAALEHTLRLVLLERFEE
                     IPTLRMIRLPASAVRLRAERLMAAVPGLVAELKPGTSVIGGGSTPQQSIPTYLIALSG
                     QKAADLERALRRSSPPVISRIEDDRVVLDLRTVEPAEEPELERALQALS"
     gene            1552..2640
                     /locus_tag="VFQ79_25205"
     CDS             1552..2640
                     /locus_tag="VFQ79_25205"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0143044.1"
                     /translation="MRRFRFMAALTMVAFVLGIPAEGQRNRKEEEPRTQVLELPKDLP
                     NLITAETDRLVFATSPLVTSGLLSRQVREALKSLNRQLRGATVVALRAFVAGTGDMRR
                     VQSIVSETFTERRQPLPVLTVVHSGGLPATGAQVVLEAVAVARKAVNPNGLVFISGQR
                     ATSIDPLDPMGPLSEKSLAVLSDTLKSTGVEAQNVQQVTCFVSLIEGSEPLRQKLAAA
                     YPKAALNIVQLRREPTGSVVECEAVAHLGKASPERIQRSSNVVLLNAPRVAITGAQLA
                     FRSQESDIKLAFERLNKTLEEAGADTGDVSIRRIYALSRQVAGRVLQIEGEYFNKQHP
                     PGGSVLLFEALPSLDASFAIDAVAELNK"
     gene            complement(2652..3080)
                     /locus_tag="VFQ79_25210"
     CDS             complement(2652..3080)
                     /locus_tag="VFQ79_25210"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0143045.1"
                     /translation="MNRSGLAGWGIMLLRVTVAVIFIVHGGQKLFVYGLGGVGQSFGQ
                     MGIPAPAIIAALVAIIEFFGGLALLLGFFTRYAALLIAAVMAGAVLLVHSSKGFFVSS
                     GGFEFALLLLVASLTLAMAGPGALSIDGSRRGASGFGRGR"
     gene            complement(3300..4058)
                     /locus_tag="VFQ79_25215"
     CDS             complement(3300..4058)
                     /locus_tag="VFQ79_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0143046.1"
                     /translation="MSEACPRCGGSGWIILEKEGFSGAERCDCVRTGRAQRLEDKAGI
                     PPLYRNASLDNFLLPRDNPNSRESLTHIVRTVRSYTKEFPCEDRPGLLLLGDPGTGKT
                     HLAVAALRMLMAKGFEGLFFDYQHLLDRIRSGYDQASGSSDKEAYRSVLEAEVLLLDD
                     LGAHRVTDWVEDTITSIVTYRCNNRKPLIATTNLPDPDAGSTVVERVPGSTSYDVRVT
                     LAERIGPRARSRLFEMCRVVRMPLMEDYRVRHAR"
     gene            complement(4072..4569)
                     /locus_tag="VFQ79_25220"
     CDS             complement(4072..4569)
                     /locus_tag="VFQ79_25220"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU0143047.1"
                     /translation="MKWHVTVGGRASEFSLERSGHECRFQFSGDSNRTASVVEVEPGI
                     YSVLLDGRSYEAKIVPGQDGWYVDIGGCHLAVGVRDPRDAGPDAEAVEAHGVQSIAAP
                     MPGRVVRVLVAEGDPVAAGQGIVVVEAMKMQNEMQAPRTGRIASLRVREGATVSAGEV
                     LAVLE"
     gene            complement(4566..>5205)
                     /locus_tag="VFQ79_25225"
     CDS             complement(4566..>5205)
                     /locus_tag="VFQ79_25225"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683622.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU0143048.1"
                     /translation="FLEMNTRLQVEHPVTELTTGHDLVQTQFRIAAGEPLGLRQEDVE
                     WRGSAIECRIYAEDPDAGFLPSPGTVTHLGRPFGPGVRLDSGVYAGWTVPMEYDPLLA
                     KLVVWARSRQDAIARMLRALDEYYVGGIKTNLAFFRQILDDGEFRTGRLHIGFIEEFF
                     RRKAASGCPPGDVEAVAALVAVIHAGHSQSAAPVSSPSVSRWRNQGRERLLR"
BASE COUNT         1072 a         1684 c         1508 g          941 t
ORIGIN      
        1 ggccacggtg gaattgacgg tggtggagac ggaacccgcc ctgaaagggg ctacggtctc
       61 caacgtcacc aagcccgcaa agctcgaaac aggactggtg gtccaggtcc cgcccttcat
      121 caccgaaggc gaaaaaatcc gcgtgagtac ggccgaaggc acgtatcagg aaagagcctg
      181 aagggctctt tcaagctccg gctcctccgc cggttccacg gtgcgtagat caaggacaac
      241 cctgtcgtct tcgattcggg agatcacggg cggtgagctg cgcctcagcg cgcgctccag
      301 gtccgctgct ttctgccccg agagtgcaat caggtatgtg ggtatagact gctgtggagt
      361 cgatccacca ccgatgacgg aggttccggg tttcagttcc gccacaaggc cgggaacagc
      421 ggccatcagg cgctcggccc gcaagcgtac ggccgatgcc ggcagccgga tcatgcgcag
      481 cgtcgggatt tcctcgaaac gttcaagcag caccaggcgc aatgtgtgct ccagcgcggc
      541 atacgtgagc ttgtcgaccc gtagcgcacg gaacatcggg tttctccgga ttcgttgaac
      601 caactcggcg cggcccgcga taataccggc ctgtggtccg ccgagcagct tatctccgct
      661 gaaagagacg agagaagcgc ccgcatccag cgaagcgcgt gccagcggct catcgatccc
      721 gtaaggactc aaatcgacga ggcatccgct gccgagatct tcataaacgg gaagcccgcg
      781 ttccttcgcc aggcgcgcca gttcgttcaa cgacgggctg gctgtaaaac cctcgatgcg
      841 gaagttgctc cggtggactc gcatcagcag gcgcgtgcgt tcgttgatgg cggcccgata
      901 gtcctctaag cgcgtacgat tcgtcgttcc gacctcgcgc aaaatcgcac ccgagcgcgc
      961 catgatgtcg ggaatccgga agccatcgcc gatttcgata agttctccac gcgagacgat
     1021 cacttcggag cccgcggcga gttcgttcag caccagatac accgccgccg cattattgtt
     1081 aactacaatg ccgccgtgtc cgagcaaccg ttccagcagt gccgtaacgt gcacgtcgcg
     1141 cttgccgcgc ctgccggtct ccaggtcgta ttccaggttg gagtaacctt ggagcgaatt
     1201 gtccaagggc acgggagccc ggcccagatt cgtgtgcaat atcaccccgg tcgcattgat
     1261 caccagccgc agtgaaggtt gcaggatggc cttgacggcc ctttgaacct gagtgccgat
     1321 ggaaccaacg cccgcggcct ctcctgtttt cattgccgtc ctcagcgatg ccaacagccg
     1381 gcgcgcttca tctaccagta ccttgtgcgg gatgttttca aaatcggccg cgacgccgga
     1441 gacaagctca tcgacagaag gcaggttgcg cagttcatca cgcccggtca cgattcgatc
     1501 gtactcactt cgaacacggc ctgtcgatgc ctgctaaaat ttgcggtagc aatgcgccgt
     1561 ttccgattca tggctgcgct cacgatggtg gctttcgttc tgggaattcc cgcagagggc
     1621 caaaggaaca ggaaggaaga ggagccgcgt acacaggtcc tcgaattgcc gaaggacctg
     1681 ccgaacctga tcaccgccga aaccgaccgg ctcgtattcg ccacatcgcc tttggtgacg
     1741 tcggggctgc tatccaggca agtgcgcgaa gccctgaaat ccctgaaccg gcaactgagg
     1801 ggcgccaccg ttgtcgcctt gcgagcgttc gtggccggaa ccggcgatat gcgccgcgtt
     1861 caatcgatcg tcagcgagac ttttacggaa cgccgccagc cgttgcccgt ccttaccgtg
     1921 gtgcattcgg gcggcctccc ggcgactggc gcacaagttg tgctggaggc ggtagcggtg
     1981 gccaggaaag cggttaaccc aaacggactg gttttcatct ccggccagcg agccaccagc
     2041 atagatccgc tggacccgat gggaccgctg tccgagaagt ccctcgccgt gctcagcgac
     2101 accttgaaaa gcacaggcgt ggaggcgcag aacgtgcagc aggtcacttg tttcgtgagc
     2161 cttatcgagg gcagtgagcc gcttcgccag aagctcgccg ccgcgtatcc caaagcagcg
     2221 ctcaacatcg ttcaactgcg gcgcgagcct acgggcagtg ttgttgagtg cgaagccgta
     2281 gcgcacctgg ggaaagcatc cccggagcgc attcaacggt cttccaatgt agtgttgttg
     2341 aatgcgccgc gcgtggcgat tacaggagcg caactcgcgt tccggtctca ggaatccgat
     2401 atcaagttag cgtttgagcg cctcaataag actctggagg aagccggtgc cgacaccggc
     2461 gacgtgtcga tccggcgtat ctacgcactc tcgcgtcagg tcgccggccg ggtgctgcaa
     2521 atcgagggtg agtacttcaa caaacagcat ccgcctggag gtagcgtgct gctatttgaa
     2581 gcgctgcctt cgctcgacgc atcgtttgcc atcgacgccg tagccgaact caacaagtag
     2641 ccgcgtcgaa cttagcggcc gcgaccgaat cccgaggcgc cgcggcgtga gccatcgatg
     2701 gacagggcgc ccggacctgc catcgccagt gtcaggctcg ccaccagcag cagcagcgca
     2761 aattcaaagc cacccgacga aacaaagaag cctttgctcg aatgcaccag cagaaccgcc
     2821 cccgccatga cggctgcgat caggagagcc gcgtatcgtg taaagaagcc caacagcagc
     2881 gcgagaccgc cgaagaattc gatgattgcc accagtgcgg ctatgatcgc cggggcagga
     2941 atgcccattt ggccaaaact ctgccccacg ccgccaaggc cgtatacaaa cagcttctgc
     3001 ccaccatgaa cgatgaagat caccgccacc gtcacacgca gcaacataat tccccaccca
     3061 gccagtccag atcgattcat cacagattct cctcgcatct ccgatgcgtt aactctgccc
     3121 gaagtctcgg gatatgtgta gtctacaagg cacggcaaca aacgtccagg accggcgacg
     3181 ccgctgccgc acgcgccggg ataatatgct cccatgagcg atccagaaaa gccgttacct
     3241 catcccgaac ccgtaccgga cccggaaccg gtgccccacc ccgaaccggc gccggaactt
     3301 cagcgcgcgt gccgtacccg gtaatcttcc atcagcggca tacggacgac acggcacatt
     3361 tcaaacaggc gcgatcgtgc gcgcgggccg atgcgttcgg ccagtgtgac acgaacgtca
     3421 taggacgttg agccgggaac ccgttccaca accgtactgc cggcgtccgg atcgggcagg
     3481 ttcgtggtgg cgatcaaagg cttccggttg ttgcagcggt aggtgacgat ggatgtaatc
     3541 gtgtcctcca cccaatccgt gacgcgatgc gcaccgagat cgtccagcag cagaacctca
     3601 gcctccaaca cgctccgata cgcctcctta tcgctggaac cggaagcctg gtcgtacccg
     3661 gagcgaatcc ggtccagcag gtgctgataa tcgaagaaca gtccttcaaa ccctttcgcc
     3721 attaacatgc ggagcgccgc caccgccaaa tgggtctttc ccgttcccgg atctccgagg
     3781 agcagcaggc ccggacggtc ctcgcagggg aactcctttg tgtaggagcg gacggtccgg
     3841 acaatatggg tcagcgattc acgcgagttg ggattgtcgc ggggcaacag gaaattgtcc
     3901 agcgatgcgt tccggtacaa cggtggaatg cccgctttgt cttccagccg ctgtgctctt
     3961 ccggtccgga cacaatcgca acgctcggcg ccggagaacc cttccttctc gaggatgatc
     4021 caaccggatc caccacacct cgggcatgct tctgacatgg aaactactat gctactccag
     4081 tacggccaac acttcaccgg cggacacagt ggcgccctcc cgaacgcgta aggacgcgat
     4141 gcgtcccgtc ctcggcgcct gcatttcgtt ttgcatcttc attgcttcca ccaccacaat
     4201 cccctgcccg gcagcgaccg gatccccttc tgcaacaagg acacgcacca ctcttcccgg
     4261 catcggcgcg gcgatggact gaacaccatg cgcttctacc gcttccgcat ccggccctgc
     4321 gtctcgcgga tcgcgcacgc caacggcgag gtggcacccg ccgatatcga cgtaccagcc
     4381 atcctgtccc ggaacgatct tcgcttcata gctgcggccg tcgagcagta ccgagtagat
     4441 tcctggctcc acttccacga ccgacgccgt gcggttggaa tcgcccgaga actggaagcg
     4501 gcattcgtgg ccgctacgct ccagggagaa ctcggaggct cgcccgccga ctgtcacgtg
     4561 ccatttcatc gaagcagccg ctccctgccc tggttgcgcc agcggctgac ggacggcgat
     4621 gaaacaggcg cggcggactg gctgtgtccg gcatggatga cagctaccag cgccgccacc
     4681 gcctccacat cgcccggtgg acagcccgat gccgctttgc gccggaaaaa ttcctctata
     4741 aagccaatgt gcaggcggcc cgtgcggaac tcgccgtcat ccaggatctg gcggaagaag
     4801 gcgagattgg ttttgattcc gccaacgtag tattcatcca gcgctcgcaa catcctggcg
     4861 atagcatcct gccgcgaacg tgcccacacc acgagcttgg cgagaagcgg atcgtattcc
     4921 atggggacgg tccatccggc gtagacaccc gaatcgagcc ggacgcccgg tccgaacggc
     4981 cgtccaagat gcgtcacggt tccaggcgag ggaagaaagc cggcatcggg atcttcggca
     5041 taaatacggc attcgatcgc cgagccgcgc cattcgacat cctcctgccg caagccgagc
     5101 ggctctcccg cggcgatgcg aaactgcgtc tgcaccaggt cgtggccagt cgtcaattcc
     5161 gtcaccgggt gttccacctg caatcgcgtg ttcatctcga ggaag
//
LOCUS       DASTMK010000612         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_191008, whole genome shotgun sequence.
ACCESSION   DASTMK010000612 DASTMK010000000
VERSION     DASTMK010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191008"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFQ79_25230"
     CDS             complement(<1..480)
                     /locus_tag="VFQ79_25230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143049.1"
                     /translation="MFVLLCGTACTPFWRKKASAAPPAPPPQVTVPEAPSGKPTPPLI
                     PPPPETPPAKLESAIEVPPPGPLPAPPNNSQRKPHRPESTPSAAGPATETSPDPPLPQ
                     LGQILTPEQRAEYNRNIDQSLHRAEKTLRVASGRTLTREQAASVAMIASFIRQAESLR
                     "
     gene            complement(530..2416)
                     /locus_tag="VFQ79_25235"
     CDS             complement(530..2416)
                     /locus_tag="VFQ79_25235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0143050.1"
                     /translation="MSLKARIRMSIVVLVAGIVIILSLVHLNQIAQSLFTDVLSRSEM
                     ITQQVRGFLTQRVNERTAALSHPPRSQEEVKKLWTKLVADDPALADLLQQMMAKTISI
                     VEVSVHDSTGKVIASSDPDRLGQTAWDLPSLEEWNRKTAWSKFTDLFTSQQNYRVSVP
                     LGFPNTQETVFTIDAIVSPVLLRFAVVPQVRDVAVLSLVAIVLSMALSVAFSNVMLRP
                     LARISAAIDRIASGESVVEVEQPSRESREFAEVQSKLDLLGQQFRGARQDALGLRTNM
                     QQLFERLEEAVLLFDGDLRLIMAGRPAERFLGKSRWEMAGRSIEEIFPASTPMGSTIQ
                     NAIQFNRPLKEHLFSTNSNDTSVGQTLVSVEILESFPSRKVAGALVTLADAETRSEVQ
                     SQLDVSSRLAAISRLTGGAAHEIKNPLNAITLHLEVLKTRIANEGTDAPELEVIEREI
                     RRLDRVVKTFLDFTKPVQLNLQEVDLCQMIGEIGSLVQPEAGQKGMRVDLEPGQGNCI
                     ISGDYDLLKQAILNIVMNGLQAMEAGGVLRVGLEEGEWGYSLRITDQGAGMTPEVKEK
                     IFNLYFSTKGGGSGIGLAMTFRVVQLHGGTIDVNSEPGKGSTFILRLPRRRRRAFASR
                     GIEA"
     gene            complement(2603..4024)
                     /locus_tag="VFQ79_25240"
     CDS             complement(2603..4024)
                     /locus_tag="VFQ79_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0143051.1"
                     /translation="MGNANDGPSTRVLIVDDEESQRAGLSAMISAWGFEVETAADGQE
                     ALDKLSSSSVHVLVTDLMMPRMDGFELLRRLGTQGAMIPAIVLTAFGNIETAIATIHD
                     LGAFWFLEKPIAPSALRVLLDRAAMHGKLAEETERLQRQLSYQGVLGEMVGTSAPMQQ
                     VFSFVRQVAPSKAAVFITGESGTGKELVARAIHTLSPRRNGPFVAINCAAMPETLMES
                     ELFGHEKGAFTGAVERRAGCFELAQRGTLLLDEIGDMPVGTQAKLLRVLEDSRVRRLG
                     GKNEIVVDVRVIASTNKILEESLRKGELREDLYYRLNVFQVQLPPLRQRLDDIPILVE
                     SLLAALNKKHSCRIVDIHPDVLERLRRHNWPGNVRELRNILERAVVLAGEGTIMPAHL
                     PSVLGATGTSKPMTDSQDPDFVKLPVGITVSDAEKALIQLTLQHTKNNKTRAAEILGI
                     SLKTLFNKLKEYGNAAESQQSGV"
BASE COUNT          830 a         1259 c         1158 g          877 t
ORIGIN      
        1 tcgtaagctc tcggcctggc gtatgaagct ggcaatcatg gccaccgagg cggcctgttc
       61 cctggtgagc gtccgcccgc tcgcaacccg aagcgtcttc tcggcccggt gaagagattg
      121 atcgatgttg cggttatact cggcgcgctg ctccggggta agaatctgtc ccagttgtgg
      181 caaaggcgga tcaggcgatg tttccgtcgc cggaccggcg gccgaaggtg tggactccgg
      241 acggtgtggc tttcgctggc tgttatttgg cggtgcgggc aacgggcccg gcggaggcac
      301 ttcaatcgcg ctttccaatt tcgccggtgg agtttcgggt gggggcggaa ttaggggtgg
      361 ggtgggtttc ccggacggcg cctccggtac cgtaacctgc ggcggaggcg cgggcggcgc
      421 cgcggacgcc ttcttccgcc agaacggagt acacgcggtg ccgcacagca gaacgaacaa
      481 gatgaataag acgattaacc tcgcaccttt aagatgcatg ggattccaat tatgcctcga
      541 taccgcggga tgcaaacgcc cggcggcgcc ggcgtggcag gcgcaaaatg aaggtactgc
      601 ccttcccagg ctcgctgttt acgtcgattg taccaccgtg aagttgaacc acccggaaag
      661 tcatcgccaa cccgattccc gatccaccgc ccttggtgct gaagtaaaga ttgaagatct
      721 tctccttcac ctcgggtgtc atgccggcgc cctggtcggt gatccgcagc gaatagcccc
      781 attctccttc ttccaggccg acacgcagca cgccaccggc ctccatagcc tgcaagccat
      841 tcatgacgat attcagaatt gcctgtttca ggagatcgta atcgcctgaa ataatgcagt
      901 ttccctggcc gggctccagg tccactctca ttcccttctg cccggcctcc ggttgaacaa
      961 gggacccgat ctcgccgatc atctggcaga gatccacttc ctggagattc agttgtaccg
     1021 gcttggtgaa atcgagaaac gttttcacta cccggtcgag ccgccgtatt tcccgctcaa
     1081 tcacttcgag ctccggtgca tccgttcctt cgttggcgat tctggtcttg agaacttcca
     1141 gatgcagcgt aatcgcgttc agcggattct tgatttcgtg cgcggcgccc ccggttaacc
     1201 ggctgatcgc cgcgagacgg cttgaaacgt caagctggct ttgcacttcg gaccgggtct
     1261 ccgcatcggc caaggtcaca agggctcccg cgaccttccg gctggggaag ctttcaagga
     1321 tttccacgct caccagcgtc tgtccgacgg aggtgtcatt cgagttggtt gaaaacaggt
     1381 gctctttcaa gggccggttg aactgaatcg cgttctgaat ggtgctgccc atcggtgtcg
     1441 atgcgggaaa gatctcctcg atgctcctgc ccgccatctc ccaccggctt ttgccgagaa
     1501 accgttccgc cgggcggccg gccatgatca ggcgcaagtc tccgtcgaac aggaggaccg
     1561 cttcttccag ccgctcgaac aactgctgca tgttggtgcg caatcccagc gcatcctggc
     1621 gcgccccccg gaactgctgc cccaataagt cgagcttgga ttggacttct gcgaattcgc
     1681 ggctctcccg gcttggctgc tcgacttcca caacggattc gccggaagca atccggtcga
     1741 tggcggcgct aatccgtgcc agcgggcgca gcatcacatt ggaaaaagcg accgacaacg
     1801 ccatcgataa aacgatcgcc accagggaga ggaccgcaac atcccgcacc tggggcacaa
     1861 cggcaaagcg cagcagcacc ggcgacacaa tcgcgtcgat ggtgaaaacc gtctcctgcg
     1921 tattcggaaa acctaacgga acgctgacgc gatagttctg ctgggaggtg aacagatccg
     1981 taaatttgga ccaggccgtt ttccggttcc attcctcgag gctgggaaga tcccaggccg
     2041 tctggcccag ccggtccggg tccgacgacg caatcacctt tcccgtactg tcgtgcaccg
     2101 agacttcgac aatggaaatt gttttcgcca tcatctgctg gagcaggtcg gcaagagcgg
     2161 gatcgtcggc cacgagtttc gtccagagct tctttacttc ctcctggcta cgcggcggat
     2221 gcgacagcgc ggctgtacgc tcgtttacac gctgtgtcag aaatcccctg acttgctgcg
     2281 tgatcatttc agagcgtgag agtacatcgg tgaaaagcga ttgggcgatc tggttcagat
     2341 gaacgagaga gagaatgatt acgatgcccg ccaccaaaac gacgatcgac attcgtatcc
     2401 gtgctttcag tgacatgctc gatccgtcaa cgttggttac gcggggcgct cggccccccc
     2461 aggcggctat ccggccaagc tcctgtctcc aagtccgcac caatcgctcc gtagccgtcg
     2521 cggctcagaa acaaaggact tactgagccg cgcgcgtgag caagcggtcg tgacttttga
     2581 agcgaacttc cgtgacagca cactagactc ccgattgctg ggactcggcg gcgtttccat
     2641 actcttttag cttgttgaac aacgtcttca gactgattcc aagaatttct gccgcgcgcg
     2701 tcttgttatt cttcgtgtgt tgcagtgtga gttgaatcaa ggctttttcg gcgtcgctta
     2761 cggtgattcc aacgggcagc ttgacgaagt ccggatcctg gctatccgtc atcggcttcg
     2821 aggtgccggt agcgcccagc acggagggaa gatgagcagg cataatcgtg ccctctccgg
     2881 ctaaaaccac cgcacgctcc agaatgttgc gcaattcgcg gacgttcccc ggccagttat
     2941 ggcggcgcag cctttcgagc acgtccgggt gaatatccac tatgcggcaa ctgtgctttt
     3001 tgttcaatgc cgcaaggagc gactctacca ggatcggaat gtcatccaac ctctgccgca
     3061 acggcggtaa ttggacctga aagacgttaa gccggtagta caggtcttcc cgcagttcgc
     3121 ccttgcgcaa cgactcttcg agaatcttgt tcgtggaagc gattactcgc acgtccacca
     3181 caatttcatt cttcccgcca aggcgccgca cccgggagtc ttccagcaca cgcagcaatt
     3241 tcgcctgcgt gccgacgggc atgtcgccga tttcatccaa cagcagagtc ccgcgctgcg
     3301 ccagctcaaa acatcctgca cgccgctcga cggcgcccgt aaaggcgccc ttctcgtgtc
     3361 caaacagttc gctctccatc agcgtttcgg gcattgcggc gcaattgatg gctacgaaag
     3421 gaccgttccg ccgcggactg agcgtatgga tcgcgcgggc aaccaactct ttccccgtac
     3481 cgctttcgcc agtgataaac acggccgcct tgctgggcgc gacctgccgg acgaaggaga
     3541 acacctgctg catcggagcc gacgtgccca ccatttcccc aagtacgccc tgatagctca
     3601 gttggcgttg cagccgctcc gtttcctctg ccagtttgcc gtgcattgcg gcacgatcga
     3661 gcaatacccg gagagcgctc ggagcaatgg gcttttccag aaaccagaac gccccgagat
     3721 cgtgtatggt cgcgatggcc gtctcgatat tgccgaaagc ggtcaacaca atagccggaa
     3781 tcatggcgcc ctgcgtaccc agacggcgga gcaactcgaa gccgtccatc cggggcatca
     3841 tcaggtccgt aaccagaaca tgaaccgatg aagacgacaa cttatcaagg gcctcctgac
     3901 catccgccgc cgtttccact tcaaaccccc aggcggatat cattgcggac aaaccagcgc
     3961 gctggctctc ttcatcgtcg acgatcagga cccttgtgga agggccgtca tttgcattgc
     4021 ccatgcttta gcttcaattg tatcgtgcgt aacaagaaca aggcggtaaa gattaccatg
     4081 caggatcaaa ctggtacaat tcagactttg gggatcgggg gctt
//
LOCUS       DASTMK010000613        10216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_127675, whole genome shotgun sequence.
ACCESSION   DASTMK010000613 DASTMK010000000
VERSION     DASTMK010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127675"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1057)
                     /gene="lpxD"
                     /locus_tag="VFQ79_25245"
     CDS             complement(17..1057)
                     /gene="lpxD"
                     /locus_tag="VFQ79_25245"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU0143052.1"
                     /translation="MLVKDLAVSLGCEVHGDGNIEITGVAGMEYAGPGQLTFLANPKY
                     AHKVRNTRASAILVTEAVKDLPIVSLISANPYLDFARALEHFYQAPRPAAGIHPLASV
                     AASAAIGAGASIGPFAVVGERVRIGRNAVLHPHVVIYEGAEIGDDFLAHSHAVVREYC
                     RIGHRVILQNGAVIGGDGFGFAKRADGTHYKIVQSGATVIEDDVEIQCLTAVDRATVG
                     ETRVRRGAKIDNLVQIGHASTVGEDNIICAQTGLAGSSVLERNVLLAGQVGVSGHLTI
                     HDNAVVYAQSGIGGNVEPNACVSGSPAFESRQWLRAVTSFPKLPDLVKTVRELERQVS
                     QLQSTRGSTSDE"
     gene            1106..3907
                     /locus_tag="VFQ79_25250"
     CDS             1106..3907
                     /locus_tag="VFQ79_25250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143053.1"
                     /translation="MKFGVSRLVPVIISPVLLLADAQQPSPAMGKAVEEFKVQSRALG
                     LREDAAGRKRGNGTTKPAWHGRIFENLRNDLFDAVPHEITQGGGSKSLLRRNQFGFNV
                     TGPLYIPRIYDGSRRTFFSVSYEGVRERISRSYLRDIPLTEERSGDFSSVVDQAGNVL
                     PVFDPASTRLNPAYDASQPISLDNLQYLRNQFPGNVIPASRLDPVAVEAVKHYPQPNT
                     SIGPFFQNNYFVVSPEENTANGMIFKLDHTISDRNRISIGTSFSNGFAGAAWFFPTPA
                     NPGAADRSFSSRRASFEHVVTFSPRTLNTFGFEAASDRSETVPEEGEADALKFGGFPG
                     DSFPVFRLGSYLSMGRSTPVSSNARNNYTASDSFSTRRGKHNVLFSGRVSRQQVNIFA
                     SQYPAGALRFGEGLTSLPGIINTGHAFASFMLGMAEFAETTYITSPSYFRRSSARVGF
                     RERYEAMPGLNFTASLYLDVNTPRVEKYNRQSTVDFSAINPANGRPGALVAAGRDGEG
                     SAFQPLTARLEPSASMSWNLGGDSRRVFRVSFARSYAAIPVYSVQFGTQGFNGAPAYL
                     SPNVQLDPALRLSDGLPPPPPLPDLRPDAANDTVADLTDRSDRLPTYQSGTVSFEREL
                     PASFIVTLGISYSGGKNLLVGSSAADPNAIPLEALRYRDLLNDEDFNRSLRPFPQYKG
                     FEVYYAWPIGRYEREEGFLTIEKRSSMGLSLRANYGFAKQMDNYSGPYGIQDYYNRDN
                     EWSMTAGSNPHRFSLTYMYELPMGPNRPLLNYSDWRRFFTEGWAISGMTSVSSGEPLA
                     LRPQFNNTGGVVRSLRVNVVPGVDPHISNPTAELWFNPAAFSHPEDFSTGNASRTHPT
                     LRGPLSQNHDLSLTKRFALATDRTLELSAVGFNFLNHANWTDPDTQIGPESAPNVNAG
                     KIIGSRGGRVIQLGLRLSF"
     gene            3904..4839
                     /locus_tag="VFQ79_25255"
     CDS             3904..4839
                     /locus_tag="VFQ79_25255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0143054.1"
                     /translation="MIRLSAPVTAVAAGAVLLAALGAQPDKARAIRVALTTDAAPPLS
                     KGEVLANVDGRKSPILAIHGPKDDLMLILVLDLTEDISNIEPAKTALISAINQLPANV
                     YVGVMRAQDGLQVLVDPTGDREAVQKAILSLPVSGRAGLLESIEPVAQLGDVVLTKAP
                     IRLAALYVTDSDIYNYREDFTNPVVNTSDYRDLSRRFPEGLIREKVAKLETSLASYQT
                     PIYIVHLVYRTDRLNEAYQSGLLQLAGATGGSAIFCRSRPEIAPAIASAVASIGSQYT
                     IDLQLPERVSKNIQIEFQSEGRVLNYRTRFIVRER"
     gene            4842..5672
                     /locus_tag="VFQ79_25260"
     CDS             4842..5672
                     /locus_tag="VFQ79_25260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143055.1"
                     /translation="MLVPQRVPFWTLAAVTAAALVLAALLVWRVTVTSRGPLIGGADA
                     TLENRVAELAAENQELRQRLDAAEHELKAAGQASGRKERPAVPEPSRDAEMVVQLRGE
                     ISSANQTIADLQNRVAELEQSVKNAAQENAQLAVRQQEIRDNLEASKRLVEALKTEVA
                     GKAERITDLERNYGKVRNDYRTAEDRVNRLLRLSRDLEDLNRRREVYVGNMLRRYRDV
                     TDQYRALADRLDNSGTGTSGASGELSRIQNSIQMAEEDLRQLSALNLEAAKLGKLLAR
                     "
     gene            5779..6075
                     /locus_tag="VFQ79_25265"
     CDS             5779..6075
                     /locus_tag="VFQ79_25265"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEU0143056.1"
                     /translation="MATTKVTFTLDPATIQRLQEAAERLAVPKSEIVREAIMEFHDRL
                     GRLSERERVSMLRTFDELVPRIPARSTKAVNRELAELREARRSGGRRTAMRKRA"
     gene            6072..6458
                     /locus_tag="VFQ79_25270"
     CDS             6072..6458
                     /locus_tag="VFQ79_25270"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0143057.1"
                     /translation="MIVLDTSALIASLSGAKPSAGILRSMIERGERILLPSLVLYEWL
                     RGPRVAQEISAQDALFPTESVLVFGPREAKLSAQLYKSLKRPRGREIDLAIAAYAIVR
                     EADLWTLNLADFKDIPHLRLVIPEDR"
     gene            complement(6459..8294)
                     /locus_tag="VFQ79_25275"
     CDS             complement(6459..8294)
                     /locus_tag="VFQ79_25275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0143058.1"
                     /translation="MKVFSACGLFLLAAAVTAAQQAPLYTVEVVRDVMVPMRDGVRLA
                     TDLYLPAQGGTRAAGKFPAMLCRTPYNKSGQGNTAEWYAQRGYAVVTQDVRGRFRSEG
                     QFYPFIQEGPDGYDAIEWVARQEWSNGKVGTYGASYLAWDQYRAAMERPPHLVAMFAN
                     VGGANFYQEYAYPGGALNLGWALWILRSAETSPAAQENAEAKSRLTQILEQPEKWLRL
                     PPRERAAVFEPFPVHLRIYEDFLAHSRHDAYWHDPGFHTPAGWERIKDVPILFISGWY
                     DYFGDGVLSNFSALSKTQKMPKKLVMGPWWHAVGSSSCGDGFFGDAAAVAVREMTLAW
                     FDRWLKGRAAGEMPDQPVRYFRMGGGPGGRDAEGRFQHGGTWLTATTWPLESLERTKL
                     YLAEGGKLNRTASAGPALTFVFDPAGPVPTIGGRYGVRSLTPDCFQDQVCRPGILGCN
                     DSSPLAERKDVLMFSTDALTEDLEVTGPVRARLWVSTDAADTDFTAKLIDVYPGGYAA
                     ILADGQVRLRYRNGFGKESLVTPGEVAEIEIELGSTSNLFAKGHRIRVDISSSNFPRF
                     ESNPNTGEPVNAGTEKKKATNTVYLDAKRPSHVELPVAPLRARSR"
     gene            complement(8331..9437)
                     /locus_tag="VFQ79_25280"
     CDS             complement(8331..9437)
                     /locus_tag="VFQ79_25280"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEU0143059.1"
                     /translation="MITRRQFGARLSAALACAPVRAASYAKPPYPESPVIGSVEFDWS
                     THKRDADGSDNWQFTWADDDHQYGAWGDGGGFGGTNSNGRVSLGVARIEGPWQNYTGV
                     NVWGGKNALNPATVDGKSWGMISVRGVLYMWVVPGSPLNAMQREARLYRSRDHAATWQ
                     PADWAMVRHEDLSIPTICQFGRDYSGARDRFVYHYFIHPREEKSDTIQRPGTVYLARC
                     PDESLMDRSTYEFFAGIAGGRPTWTKNIAQKRPVFEDRENGTGWVLSVTYNQGLRRYL
                     LMTDHITSNRGNLGIFDAPEPWGPWTTVLYLDEARGTNFGAGRIEPNTFFWNMPTKWQ
                     SRDGLDFTMVFTGSGRGKNNDSFNLVRGRFEIRK"
     gene            9531..10025
                     /locus_tag="VFQ79_25285"
     CDS             9531..10025
                     /locus_tag="VFQ79_25285"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0143060.1"
                     /translation="MPMQSDAFIVDAGETRTGGPLFLLGETISIKITGRDTGGKYTIF
                     ETTTPVNGGPPLHMHHREDEWFYVLEGEFLFQVGDRRIRAATGAALLAPHDVPHTFLN
                     VGTTPGRTLVVAEPAGLEMFFAQMAAACVDGLPDPAAILPVFEKYGLELLGPPLTAHA
                     ESAG"
BASE COUNT         2009 a         3135 c         2998 g         2074 t
ORIGIN      
        1 gagagaggtg atttcatcat tcgtcggatg tgctcccgcg ggtggattga agttgactga
       61 cctggcgttc gagttcccgg actgttttca ccaggtcggg cagcttcggg aaactggtta
      121 ccgcgcgcag ccactgccgg ctctcgaaag cgggggagcc ggagacgcaa gcgttaggtt
      181 caacattgcc gccaatgccg ctttgcgcgt agaccacggc attatcgtga atggtgaggt
      241 ggccggaaac gccaacctgc ccggccaaca gtacgttcct ctcgagcacc gagctgccgg
      301 ccaatccggt ttgagcgcag ataatattgt cttcccccac cgtcgatgca tgaccgatct
      361 gaaccaggtt gtcgatcttg gcgccgcgcc gcactcgcgt ttcgcccacc gtcgcgcggt
      421 ccacggccgt caggcattgg atttcgacat cgtcctcgat taccgtcgcg cccgattgaa
      481 cgattttgta gtgcgtgccg tcggcacgtt tcgcaaaacc gaagccgtcg ccgccgatga
      541 cggcgccgtt ctgtagaatc acgcgatggc cgatgcggca gtattcccgg accacggcgt
      601 gcgaatgggc gagaaaatcg tccccgattt cagcgccttc gtagatgaca acgtgcgggt
      661 gcagcacggc attccggccg attcgaacgc gttcccccac gacggcgaac ggaccgatgg
      721 acgctcccgc cccgatggcc gcggatgccg cgaccgatgc cagcggatga atgccggccg
      781 ccgggcgcgg cgcctgatag aaatgctcca acgccctcgc aaaatccaga taggggttgg
      841 ctgaaatcag cgagacgatg ggaaggtctt tcaccgcctc cgtcaccaga atggcgctcg
      901 cgcgggtgtt ccgtaccttg tgggcgtact tcggattggc cagaaacgta agctggcccg
      961 gtccggcgta ctccattccc gctacaccgg taatttcaat gttgccgtcg ccgtgcacct
     1021 cgcagcccag cgatacggcc aaatccttga ctaacatggc ttcttttcag ggtaacgcga
     1081 cctgcacggg ttaaactaga agtgtatgaa gttcggggtg agccggctcg tgccggtcat
     1141 catcagccca gtcctgctat tggccgatgc acagcaaccg tccccagcca tggggaaggc
     1201 tgtcgaggaa ttcaaggtac agagccgcgc gttaggcttg cgtgaagacg cggccggaag
     1261 gaagcgcgga aacgggacca ccaagcccgc ctggcacggc cggattttcg agaatctccg
     1321 caacgatctg ttcgacgccg tgccgcacga aatcacgcag ggcggcggct ccaaatcgct
     1381 tctgcggcgc aatcagttcg ggttcaatgt aacggggccg ctttacattc cgcgcatcta
     1441 cgatggaagc cgccggacct ttttctcggt ttcctacgaa ggcgttcgtg aacggatttc
     1501 acgatcctac cttcgggaca tccccttgac ggaggaacgg agcggcgatt tttcttcggt
     1561 cgtggatcag gccggcaacg tgcttccggt tttcgatccg gccagcacgc gcctcaatcc
     1621 ggcttacgat gcttcgcagc caatatctct cgacaatctg caatacctgc gcaatcaatt
     1681 tcctggtaat gtgatcccgg ccagccgcct ggatccggtg gcggtagagg cggtgaaaca
     1741 ttacccgcaa cccaacactt ccatcgggcc gttctttcag aacaactact tcgtggtgtc
     1801 accggaagaa aatacggcta acggcatgat cttcaagttg gatcacacca tctccgaccg
     1861 gaaccgcatt tcgatcggca cgagtttttc gaacggtttc gcgggggcag cgtggttctt
     1921 cccgacgccc gccaatcccg gcgccgccga ccgttcgttc tcgagccgcc gtgcttcctt
     1981 tgagcacgtc gtcacctttt cgccccgaac cctgaatacc ttcggttttg aggctgccag
     2041 cgaccggtcg gaaaccgttc ccgaggaagg ggaagcagac gctctcaaat tcggcggatt
     2101 ccccggcgac tcgttccccg tgttccgtct cggcagctat ctttcgatgg ggcgctccac
     2161 gccggtttcg agcaatgccc gcaacaacta tacggcttcc gattcgttct cgacacgccg
     2221 cggcaagcac aacgtgcttt tctcgggcag ggtatcgcgc cagcaagtga atatattcgc
     2281 ctcccagtat cccgccggcg cgctgcgatt cggcgaaggg ctcaccagcc tgcccggcat
     2341 catcaatacg gggcacgcgt ttgcgagctt catgctcggt atggcggaat tcgccgaaac
     2401 gacctacatc acgtcgccat cgtactttcg gcgcagtagc gccagagtag gcttccgaga
     2461 gcgctatgag gcgatgcccg gactgaattt taccgcctcg ttgtatctgg acgtcaacac
     2521 tccccgagtg gagaaataca accgccaatc gacggtcgat ttcagcgcga tcaatcccgc
     2581 caatggccgg cccggagctc ttgtggccgc cggtcgcgac ggtgagggga gcgctttcca
     2641 gccgctgacg gcgcgattgg agccgagcgc gagcatgtcc tggaaccttg gcggcgactc
     2701 ccgaagagta ttccgggtca gttttgcacg cagctacgcc gcgattcccg tctattcggt
     2761 gcagttcggc acgcagggtt tcaacggcgc gccggcttat ctttcgccaa acgtgcaact
     2821 ggatccggcg cttcgtctca gcgacggctt accgcccccg ccgccgctgc ccgatcttcg
     2881 gcccgatgcg gccaacgaca cggtagccga tctcaccgac cggtccgacc ggttgccgac
     2941 gtatcagtcc ggcacggtga gtttcgagcg tgagttgccc gcttccttca tcgtgacgtt
     3001 gggtatctcc tattcgggtg gaaagaacct cctggttgga agttccgccg ccgacccgaa
     3061 cgcgatcccg ctggaggcgc tccgttatcg cgatctgctg aacgacgaag acttcaaccg
     3121 ttcgctgcgt ccctttcctc agtacaaagg cttcgaagtt tactacgcgt ggccgatcgg
     3181 aagatatgag cgggaggaag gtttcctgac catcgaaaag cgaagttcca tgggcctgtc
     3241 gttacgcgcg aactacgggt tcgccaagca gatggacaac tattccgggc cctatggaat
     3301 tcaggactat tacaaccgcg acaacgaatg gtcgatgacc gccggcagca acccgcaccg
     3361 cttctcgctc acttacatgt acgaacttcc aatgggaccg aaccggccgc tgttgaacta
     3421 ctcggattgg cggcgttttt ttaccgaagg gtgggcgatt agcggcatga cgtccgtttc
     3481 gagcggcgag ccgcttgcgc tccgtccgca gttcaacaac accggaggcg tggtccggtc
     3541 tctgcgtgtg aatgttgtcc cgggcgtcga cccgcacatt tcgaatccca cggccgagct
     3601 ttggttcaat ccggcggcat tctcgcatcc ggaagacttt tcgaccggca acgcgtcgcg
     3661 aacgcatccc acgctgcggg gaccgctgag ccagaatcac gacctctcgc tgaccaaacg
     3721 ctttgcgctg gcaaccgacc gcacgctgga gttgagcgcg gtcgggttca actttctgaa
     3781 ccatgcgaat tggactgacc cggatactca gatcggtccg gagagcgcgc cgaacgtgaa
     3841 tgccggaaag atcatcggtt cccgcggagg ccgtgtgatt cagttgggtt tgaggttgag
     3901 cttttgatac gtctttccgc cccggttacg gcagtggccg ctggagccgt gctgctggcg
     3961 gcactcggcg cccagcctga caaggccaga gcaatccggg tagccctcac cacggacgcg
     4021 gcgccgccgc tgtccaaggg ggaagtgctg gcgaacgtcg acgggcgcaa gtcgcctatt
     4081 ctcgcgatac acggaccgaa agacgatttg atgttgattc tcgtcctcga cctgacggag
     4141 gacatatcga atatcgagcc ggccaagacg gcgctcatct ccgccatcaa ccaactcccg
     4201 gccaatgtat acgtgggagt gatgcgggcg caggacgggc ttcaggtcct ggtggatccc
     4261 accggcgatc gggaggctgt tcagaaggca atcctgagtt tgcccgtgag tggccgcgcg
     4321 gggttgcttg aaagcatcga acccgtggct caactcgggg atgttgtgct gacgaaagcg
     4381 cccatccggc tggccgccct ctatgttacc gatagcgata tctacaacta ccgggaagac
     4441 ttcaccaacc cggtggtgaa tacgagcgac taccgcgact taagcagaag atttcccgaa
     4501 ggtctcatcc gtgagaaagt tgctaagctg gaaaccagcc tcgcttctta ccagacaccc
     4561 atttacattg tgcatctggt ttaccggacg gaccgcttga acgaagccta tcaatcgggt
     4621 ctcctgcaac ttgccggcgc caccggcgga tcggcgatat tttgccgttc gcgcccggaa
     4681 attgcgcccg cgatcgcttc ggccgtggcc tccatcggca gccagtatac gatcgatctt
     4741 cagcttcccg agagagtttc aaagaacatc caaatcgagt ttcagagtga aggccgcgtg
     4801 ctgaattacc gcacaaggtt tatcgtgaga gagaggtgaa tatgctggta ccacagcgag
     4861 ttcccttttg gacgctggcc gctgtgaccg ccgccgcgct ggttctggcg gctcttctcg
     4921 tttggcgcgt caccgtgacc tcgcgaggtc cgctgatagg cggtgcggac gccactctgg
     4981 agaatcgcgt cgcggaattg gccgcggaaa accaggaact gaggcagcgt ctggatgccg
     5041 ccgagcacga gttgaaggcc gccgggcagg catccggcag gaaagagaga cctgcggttc
     5101 cggaaccctc acgcgacgcc gagatggtag tgcaactccg cggcgagatc tcgtcggcta
     5161 accagactat tgccgatctg caaaaccgcg tcgcggaact cgaacaatcc gtgaagaacg
     5221 ccgctcagga aaacgcacaa ctggccgtga gacaacagga gatcagggat aacctggagg
     5281 cgagcaaacg gctggtggaa gccctcaaaa ccgaagtagc cgggaaagcc gagcggatta
     5341 cggatctcga gcggaactac ggaaaagtgc gcaacgacta tcgcactgca gaggaccggg
     5401 tcaaccgttt actgcggctc agccgcgatc tggaagatct gaaccgcagg cgggaggtat
     5461 atgttggcaa catgctgcgg cgctatcgcg atgtcaccga ccagtatcgc gcgctggccg
     5521 accgcctgga caactccgga acgggaacgt ccggcgcgtc cggtgaactg tccaggatac
     5581 aaaacagcat ccagatggcc gaggaggatc tccggcagtt gagcgccctg aatctcgagg
     5641 cggcaaaact ggggaaactg ctcgcacgtt gagctaaata catgtttcac cggccgcttc
     5701 acggcaagcg gcatccgata ggtcaccgta atgcgggccg gcgctacttc ccttatatgg
     5761 tgatagtatg gtgacggtat ggcaaccacg aaggtgacgt ttactctgga cccggccaca
     5821 attcaaaggc tgcaggaggc ggccgagcgg cttgctgtgc cgaagagcga gattgtcaga
     5881 gaagccatca tggaatttca tgaccgctta gggcgcctga gcgagaggga gcgtgtgtcc
     5941 atgcttcgca cctttgacga attggttccg aggatacctg cacggagcac aaaggccgtc
     6001 aaccgtgagt tggcggaact acgcgaggca cggagatcgg gcgggcggcg gacggcgatg
     6061 cggaagcggg catgattgtt ctggatactt ccgcgctgat cgcaagtctg tctggcgcca
     6121 aaccttcggc gggcatcctt cgatccatga tagagcgcgg cgaacggatt ttgctgccat
     6181 cccttgtgct atacgaatgg ctccgagggc cgcgcgtagc acaggaaatc tcggctcagg
     6241 atgccttgtt ccccaccgag tcagttcttg tgttcgggcc tcgtgaggcg aagttgagcg
     6301 cgcaactgta caaatcgctg aagcgccctc gtgggcggga gatcgacttg gctattgcgg
     6361 cctatgcaat tgttcgtgag gcagatctat ggacactgaa cctcgcggat ttcaaagata
     6421 tcccgcacct tcggcttgtc attccagagg accgctgact atctcgatct cgctcgcaac
     6481 ggcgccacgg gcagctcaac atgagacggg cgcttggcgt cgagatagac ggtgttcgtc
     6541 gctttctttt tctctgtgcc ggcatttacg ggctcgcccg tattcggatt cgattcgaat
     6601 cgcggaaagt tgctgctgga aatatccacg cgaatgcgat ggccctttgc gaatagatta
     6661 ctcgttgacc ccagttcgat ttcgatttcc gctacttcac cgggagtgac caggctctcc
     6721 tttccgaatc cattgcggta gcggagccgt acctgtccat ccgccagaat cgcggcgtat
     6781 ccgcccggat agacgtcgat cagtttcgcc gtgaagtccg tgtccgcggc atccgtcgag
     6841 acccacagcc tcgcccgcac aggcccggtg acctcgagat cttcggtgag ggcgtccgtc
     6901 gaaaacatca gaacatcttt gcgttccgca agcgggctcg agtcattgca tcccagaatg
     6961 cccggccggc agacctggtc ttgaaaacag tccggcgtga gggagcgaac cccgtaccgc
     7021 ccgccgatgg ttggaacggg accggcgggg tcgaatacga acgtgagcgc cggccctgcg
     7081 gaggctgtcc ggttcagctt tcctccttcg gcgagataca gcttcgttcg ttcgagagat
     7141 tcgagaggcc atgtcgtcgc ggtcagccag gttccgccat gttgaaagcg gccctcggca
     7201 tcccgcccgc ccggtccgcc tcccatgcgg aaatagcgga caggttgatc cggcatctcc
     7261 ccggccgccc tacctttcag ccagcgatcg aaccatgcaa gggtcatttc ccgaaccgca
     7321 acggccgccg catctccgaa aaatccgtca ccgcacgaag aggagcccac agcgtgccac
     7381 cacggcccca tcaccagctt tttcggcatc ttctgcgttt tgctgagcgc gctgaagttg
     7441 gacaacacgc catcgccaaa atagtcgtac cagccgctga taaagaggat gggcacatcc
     7501 ttgatacgct cccaccccgc cggtgtatga aaaccggggt cgtgccagta tgcgtcatgg
     7561 cgcgaatgtg cgagaaagtc ttcgtagatg cgaaggtgca ccggaaacgg ctcgaaaaca
     7621 gccgcacgct cgcgcggagg cagcctgagc catttttccg gctgctcgag aatctgtgtc
     7681 agtctgcttt tcgcttcggc gttttcctgc gcggccggac tggtttccgc cgaccttaga
     7741 atccaaagcg cccatccgag attcagagcc ccgccgggat acgcgtactc ctggtagaaa
     7801 ttggcgcccc cgacgttggc aaacatcgca accagatgtg gtggacgttc cattgcggcc
     7861 cgatattgat cccaggccaa ataggatgcg ccgtaagtcc ccaccttacc gttcgaccat
     7921 tcctggcgcg ctacccactc aatcgcgtcg tagccgtccg gcccctcctg tataaatgga
     7981 tagaattggc cttcggaacg gaaccggccg cgcacatcct gcgtaaccac cgcgtatccg
     8041 cgttgcgcgt accactcggc agtattcccc tgacccgatt tattgtatgg tgtgcggcac
     8101 agcattgccg gaaacttacc agccgcgcga gttccccctt gtgcgggcag atacaagtcg
     8161 gtcgcgagcc ggacgccgtc ccgcatgggg accatgacgt cgcggaccac ctccacggtg
     8221 tagagcggcg cctgttgtgc cgcggtgacg gcggccgcca gcaggaaaag cccgcaggca
     8281 gagaaaactt tcatagatgc atcatctccc gttccgggat ttcatgcacg ctattttcgt
     8341 atttcgaacc tgccgcgaac gaggttaaag ctgtcgttgt tctttccgcg gccggaaccg
     8401 gtaaacacca tggtgaaatc gaggccgtcc cggctctgcc atttggtggg catgttccaa
     8461 aagaacgtgt tcggctcgat ccggcccgcg ccgaaatttg ttccccgggc ttcgtcgagg
     8521 tatagaaccg tcgtccaggg accccacggc tccggtgcgt cgaagatgcc caggttgccg
     8581 cggttcgaag tgatatggtc cgtcatcagc aggtaacgcc tgagcccttg attgtaagtg
     8641 acactcagga cccagccggt tccgttctcg cgatcttcga acacgggccg cttctgtgca
     8701 atattcttcg tccaggttgg ccggccgcct gctatgccgg caaaaaattc gtacgtgctc
     8761 ctgtccatca agctttcatc cgggcagcgc gccagataga ccgttccggg ccgttggatg
     8821 gtgtcgctct tctcctcgcg cggatggatg aaatagtgat agacaaagcg gtcgcgtgcg
     8881 ccgctgtaat cgcggccgaa ctggcagatg gttggaatcg acagatcctc gtggcgcacc
     8941 atcgcccagt ctgccggctg ccaggttgcc gcgtggtcgc gcgaccggta taagcgcgcc
     9001 tcgcgttgca tcgcgttcaa aggcgagcct ggaaccaccc acatgtaaag aacgccgcgc
     9061 accgagatca tgccccagct cttaccatcg acagtggcag ggtttagggc gttcttgcct
     9121 ccccacacat tgacgccggt gtaattttgc cacggccctt caatgcgtgc gacccccagg
     9181 ctcacacgtc cattggagtt ggttccgccg aatccgccgc cgtcgcccca ggctccgtac
     9241 tgatgatcgt cgtccgccca cgtgaactgc cagttatcgc tgccgtccgc gtcgcgcttg
     9301 tgcgtgctcc aatcgaactc gaccgacccg atgaccggac tctccgggta tggcggcttt
     9361 gcgtaacttg cggcgcggac aggagcgcac gccagtgccg ctgacagcct ggcgccaaat
     9421 tgccgtcttg tgatcatctg gttgccaatg cctcctattt tctacggcag caccgatgca
     9481 tgaaccgtgc tattgtatgt cagttcaaaa ttttcagagg agcgtgcctg atgccgatgc
     9541 agtccgatgc tttcatagta gatgccggtg agacccgtac gggaggaccg ctgtttctac
     9601 tgggcgaaac aatctcgatc aagatcaccg ggagagacac cggcggcaag tacaccatct
     9661 ttgagactac gacaccagta aacggaggcc ctcccctaca catgcatcat cgtgaggacg
     9721 aatggttcta cgtgctagag ggcgagttcc tgttccaggt tggcgatcgg cggatccggg
     9781 ctgcaaccgg cgctgctctt ctcgctccgc acgacgtacc gcacactttt ctcaacgtgg
     9841 gtacaacacc gggccgcacc ctggttgtgg ccgagcccgc cggacttgag atgttcttcg
     9901 cccagatggc tgcggcgtgc gtggatggcc tgcccgatcc cgccgcgatc ctgcccgtct
     9961 ttgagaagta tgggcttgag ttgctgggcc ctccgttgac ggcgcacgcc gaatcggctg
    10021 gctgacaacc tcagacggta gggctacttc aggaacttca cccggcaccc ttcagagcaa
    10081 aagtagacgg tttcacccgc cggcgtccgc ccgctgagag cggaattcac gggcacgtag
    10141 gtaccgcaaa ccggatcttt cttgagttca ccaccggcga ctttgccgcc acgggacccc
    10201 tttgccgtgc ctccgg
//
LOCUS       DASTMK010000614        17853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_255428, whole genome shotgun sequence.
ACCESSION   DASTMK010000614 DASTMK010000000
VERSION     DASTMK010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17853
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255428"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFQ79_25290"
     CDS             <1..278
                     /locus_tag="VFQ79_25290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143061.1"
                     /translation="AVVFAGNHPDAAERLLAAVRYQANLTWNEAPAAQPENIGTLILH
                     IMLLIGLLLAVSVVLGFAFGGLRVFRHRWLGSKNADEAMITLHLGDR"
     gene            627..2297
                     /locus_tag="VFQ79_25295"
     CDS             627..2297
                     /locus_tag="VFQ79_25295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU0143062.1"
                     /translation="MADSLLQVIPLGGLGEFGMNCMALRYGDDIIVIDAGMMFPDAEL
                     LGVDIVTPDFTYLVENREHVRAVILTHGHEDHIGGVPFLLAELNVPIYGTAFTLALVA
                     RRLEEHEVQDTVRLNRVKPGERVTIGAFEIEFINVTHSIVSALALAIRTPLGVVIHTG
                     DFKVDPTPTDNLLFDLHTFAHYGKQGVLLLLSDSTNVDRPGYTESELAVRPRLEDILT
                     RAEKRVVIACFSSSIHRLQQILDLSAQAGRKVAFLGRSMLTTTEIAHHLGLLSIPDGI
                     LLRPQDINQTDRSKVTVVISGTQGEPMSSLSRVAVDSHKHLSIQPQDTVVLSSRIIPG
                     NEKAIYRMINHLARRGADLVYGSMNPPIHVSGHASAEELKLILNLVRPRYFVPIHGEY
                     RQLAKHASLARHLLHQGMERSFILETGETLEIDRRGARKGEKVTVGRVCIDSGSVDEV
                     VEDIVIRDRRHLSEDGIVLPIIAIDKNTGKPEGLPEIVSRGFVARENGSEILQDARQV
                     VARTLEVSSPEERADWGVMQEKIRADLKRFLNKQTSRRPLIMPVILEV"
     gene            2373..2705
                     /locus_tag="VFQ79_25300"
     CDS             2373..2705
                     /locus_tag="VFQ79_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0143063.1"
                     /translation="MAKNSELLQGTLDLLILKALALGSNHGLGVSRRIAQITQGTFDV
                     KPGSLFPALHRLEEKGWLVAEWGQSENNRRAKYYRLTQAGRRRLDTEVNAWRRTSGAI
                     TRAIEATS"
     gene            2717..5449
                     /locus_tag="VFQ79_25305"
     CDS             2717..5449
                     /locus_tag="VFQ79_25305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0143064.1"
                     /translation="MQMFEQLKALVRNVTGKARVEQDLDEEVRAYLDMTIDEKVSRGV
                     SRDKALREAWIELGGAEQLKEEVRAAKLGASLEKTWRDFRFALRLFARNPGFTAVIVL
                     SLGLCIGANTAIFSLVNALLLKTLPVKEPERLVLYSDGRARGFISGQSGRWNIFSYPL
                     YRRFREPGTSFEDVAAFRTQLDRLSLRAHGTGGGEGAQLTWGRLVSGNYFSVLGVKAA
                     LGRGLTADDERLAAVPVAVLAFDYWKRQYESSPSVVGQVIDINGVPVTIVGVAPAEFF
                     GESVESQLADVWLPLTLQPRIMQRQSALEDAEANWLNLIGRLRPGSTMVQAQAEVSAR
                     FQQFLIELAGANLTGEQQQQLQRNHVVLTPGANGISALRVRYSRALRLLMGVVGFVLL
                     IACANVANLLLSRAAAREKEMSMRLALGAGRGRLLRQLLTESVLLTCMGGALGLLLSG
                     WMARALVAGVSTGDRTVPLNLSPDASVLGFTAGVCILVVILFGLAPALRTSRVDLMTA
                     LKASGAAGSGRSRRGLARGFVIAQVALSLPLLAGAALLVQTLRELRTQDFGFSHENVL
                     EVGIDPGIAGYKPDQVGTLYRAVLDRVHSVPSVRIASLSQYSPMSGDNWSGQVQVEGY
                     TPSRGENAYCQWVWVGPRYAETLGMTLVLGRDLDKRDTENSPKVAVVNESFARRYLAD
                     QNPIGRHFSLKDTIEIVGVVKDFKFNDPRQEIWPVAFMPLVQSSFTPARFARYLEVRA
                     GGDPLSIAANIREAVKEVAPGLPVTGMKTLSQQIDTALGREDLIAGVSSSFAVLALLL
                     ACLGLYGVVSYSVAGRTKEIGVRMALGARRPNILTMVLREALLLALIGAGIGLATFLA
                     VAEAVSSQLYGLKPTDPYIMAGASLLLIAAAGLAGFIPAHRASRVDPMTTLRAE"
     gene            5551..8745
                     /locus_tag="VFQ79_25310"
     CDS             5551..8745
                     /locus_tag="VFQ79_25310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0143065.1"
                     /translation="MTLLRSVVLFVVVSTALYAQQTTGAIRGAVTDPSGASVADVTIT
                     ATNNDTGATQTTKTGAGGNYVLPLLPPGRYSVSAESQGFTKALRSNVLVRITETEVVE
                     FSLQLGSVAESVTVDDTVSLVQSETATEGRVIERQTISALPLATRNFTQLLGLTAGVV
                     TDPPNAENVGFGTQNPSVNGSRRGSNNYLLDGNVNNNPMNNDVLGVGIPSVDFIQEFK
                     VLTSMAGAEYGRNAGSVVNVVTRSGTNQFHGNLFEFLRNDKFNARPFFAARRGQNVQN
                     QFGATIGGPVWIPGVYQGRDRTFFFFGWESIRQRNTNSTAAIVIGRVPTPAERRGQFD
                     TPVRDPLTNNPFPGGVIPEARINPTSAQLLERFVPLPNTTNPTSNFLQQFGTPFDANQ
                     YTIRVDHEIGPRDRFMARYFDSNSYSFTASGRLPGFGRVSDPINRQVAASETHIFRPN
                     LLNEIRFGFYKFPSPGTDVQRIDPRSVGIEPMNDVPGLPGMLVTGFLGYGYTGNDFKD
                     EQNNFQFANVLSWISGNHSMRIGGEYRYNMMLSSGVPFQGRFAFNGQYTTNAFADFLL
                     GAPNQVIVANGPAVIDMRASNYAFFFQDDWKATRRLTLNVGLRYEYDTPPTDVALGRL
                     LNFYPERYRGPGVESGLVIGGESPGVPPSTVFPDRTNFGPRIGFAYALGSNSHTVLRG
                     GYGIFYDSRSGQVTQQKLFEPPYSANQTVVMRPADAPRGFVFPAPIDLSRPTETVPGG
                     TLSIRPIEPNTRMDYVQQWNLGIQHQFENGILVAASYVGTRGLQLFRATNINYPRLVG
                     DTFVRPYDGFTTIVQSQAGATSDYHSGQLTVQKRFSRGVSVLGAYTFGKALDDSTSGV
                     RYFTAATGDPGDLAASRGPADFDRTHRLVVSYNLDLPNPFGRGVQGVKRALLSGWELS
                     GVTTIQSGTPFSVTNAASNLDRDGQAGAPGTGGRADRVGDISQTTEGDVRSRLDHYLN
                     PTAFAPAPRSRYGNLGRNTIRGPGAHLWDARMTKLTTIREGLNLRFLAEFFNIWNHAA
                     FGNPGSVLGTPAFGTIRSTLSNARIIQFGLKLEF"
     gene            8735..10876
                     /locus_tag="VFQ79_25315"
     CDS             8735..10876
                     /locus_tag="VFQ79_25315"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0143066.1"
                     /translation="MNSRNHARLTRCALAGGAAGLLLFAAHFTQREAAGQAAVPLSKP
                     GQEDLSFWVNHNGLLQFEAEGEVGFGDIARILLRRRTNGEAIRELRDRFTFRVQDAAA
                     LTTPDVFRAEYSSGNLMWRAAPQSRFVAGSSRTVNIPVVVVNRDSQPITIDAMYAGAS
                     MESAARNIAIAPGGNTAILLRTVETSIGPSAGKLTLRHGTGEITTAIHFDVRPLVPLR
                     VRLLDERGQPAIARVYITGSDGLSYVPTGSSARITAMSAEYFFHADSDFEIQLPAGET
                     LLEATRGPEYRLTAERVHLQPGTPAEATLQLARWAHPAQKGWWSGDVHIHANYTSPHH
                     QVIEPRDVRLQVAGEDLNYANLMVANSSGAFIHDRQYFEAKPHRLSTPEHFLYWNEEN
                     RSSAYGHMCFLGLKHLVEPFYNGFRNTPHWEDYPANYTLSQQVFDQGGAVSYAHPGMA
                     PNFESASIKELPVDLALGQPTAMDVLSNNDEAATTEMWYRLLNCGFRVAISAGTDAFT
                     NVVDHYIGGGNRVYVQSGPRFDYEAWLEGFRRGRSFASNGPITTLVVNDRSPGDEILL
                     DREEEVVVRGTVSSQVPLERIDVIVNGAPVHTISPAGKETVDFTHRIVIKESSWIALR
                     ALGPRHRLILNDTQAYAHTSPVYVTVAGRPVHVADDVRFYREWVERLIARTETRGRFA
                     TPERKAEVLALFRKGLDWYRRAEAGKRERSY"
     gene            10945..11343
                     /locus_tag="VFQ79_25320"
     CDS             10945..11343
                     /locus_tag="VFQ79_25320"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0143067.1"
                     /translation="MAKPKPAIRIDLNSPVPNYRQIVDSLRTHLVDGALAPGDVLPSV
                     RRLAMELGITFNTVAQAYRQLAEEGWLELKHGRRAIVIDRHLPSSASREKVTGFRLRI
                     RQMVAQMRAEGLSPRDIARELHLLAEKLEA"
     gene            11346..12356
                     /locus_tag="VFQ79_25325"
     CDS             11346..12356
                     /locus_tag="VFQ79_25325"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HEU0143068.1"
                     /translation="MIRLYLLLPLAATLATLHVLPFLPHRDRLFGVAVPPEVRYGSED
                     RQALRRYELHLLPWTATAFFVSLWLPLSWAAVWMSAASLLPLIAAGRIFWRTRGEIQH
                     LALPASSTRQAQLTDTDSHLQRQTLLFLIPMAILAGTALYLHLHWDQIPPRFPVHWGT
                     DGTPNGWSTRSFAGVYGPLLIGAIIVLFILGISAVISWGSRRSTRPSAAQPILVVVAC
                     IIAAGCSLAGLLPLHVTPVWGLVVFNAVSFGLIAVAIWFSVRRRGETAPESGEVTPEN
                     CWHCWGQFYHNPQDPALFVEKRIGLGWTFNFGNRLSWVVLALMNLIPAGLIFLILQFT
                     KS"
     gene            12393..13568
                     /locus_tag="VFQ79_25330"
     CDS             12393..13568
                     /locus_tag="VFQ79_25330"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEU0143069.1"
                     /translation="MPVLLCSACFAANIPAGAELHVRLQNKVASNTSKPGDAVSSIVI
                     APVVVDGAVVLHSGVRLHGEVKSAKTSKGPEDRAMLEIVFNRMEDSTGQRVEIQSRVS
                     EVDNARESVDEQGRINGIVASQTISARMDQGIGKVAQRSGGLADFLGLVKGAVLKEAD
                     AEIVYEPGVEMNVKLTAPIEWKDTGADPNLVPVADPDALNRLVNSQPYQTRAEKPPKP
                     SDITNLMLIGTEEEIREAFKAAGWFTAAVLNSLSALETFRAIAESRGYSEAPMSILLL
                     DDQKAAMDFQKQNNTFAMRHHLRVWKRPDTFQGKPVWVVAATHDIGIELSPEQRTFIH
                     KIDSNIDRERAKVVSDLLFTGKVSSLALVQRPDVPRETKNATGDTVVTDGAMAVLIF"
     gene            complement(13571..14137)
                     /locus_tag="VFQ79_25335"
     CDS             complement(13571..14137)
                     /locus_tag="VFQ79_25335"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0143070.1"
                     /translation="MIRYYITDRKQLGGVEALLANIGSALASGIDMIQIREKDLTARA
                     LADLVRAALAFPNPSGTRILVNSRTDVALACGAHGVHLMSDPIPPARLRQIAPKPFLI
                     GVSCHSSEEIQRAEGADFVVFSPVFFTPSKAGYGAPQGLDKLREAVQASSLPVLALGG
                     VNELNTPLCIQAGAAGVAGISMFQRVDG"
     gene            complement(14139..14720)
                     /locus_tag="VFQ79_25340"
     CDS             complement(14139..14720)
                     /locus_tag="VFQ79_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HEU0143071.1"
                     /translation="MKAFDEYVELARLAHGHICAGQILGLRMAVYGVNLLGLEDPAGK
                     DRKRLITFVEIDRCATDAIGVVTGCRLGKRALKFRDFGKVAATFCDLQAGKAVRVVAK
                     ESSKERAKQLYPDIADKNQQQMRAYREMPDEDLFDKQWVRVTLGPEEMPGYKAPRVVC
                     EECGEGINFKREVVRNGKTLCKSCAGERYYEPA"
     gene            complement(14751..15821)
                     /locus_tag="VFQ79_25345"
     CDS             complement(14751..15821)
                     /locus_tag="VFQ79_25345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0143072.1"
                     /translation="MIRARIRKNFAAGPESSAFSLDVQLELGAGITVLFGPSGAGKTL
                     ILDCIAGFVQPDEGRILLDDAILFDGAARVNIPPRARRCGYVFQNYALFPHMSLRGNV
                     DFAAKNIPRLERHRSVTEMLERFHIVEVAGRRPHEVSGGQKQRCSIARAMIGSPKLLL
                     LDEPARGLDAPLRVELYTVLRQIRAEFGIPILVVTHDLEECFALGEEMLVLRDGRLVQ
                     TGPPRKVVEQPAGIEVARLFGIYNLLPVEIRALDPGRNTSRLRYEEFEITGPYFPGRF
                     KGDRVWLCIHPEQLTVSPADGKPGPNQVRANLLRAVEKPQAVRLEFSGDLAAEVPRSQ
                     FEGHKHNKDWVIEFPVEGLRVL"
     gene            complement(15818..16501)
                     /gene="modB"
                     /locus_tag="VFQ79_25350"
     CDS             complement(15818..16501)
                     /gene="modB"
                     /locus_tag="VFQ79_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEU0143073.1"
                     /translation="MQIDWFPLWLSLRVAALSTVIAFFGGLALAYVLANRQFRWKEVI
                     DAAVTLPLVLPPTVLGYYLLVVLGRQSPFGKLFEGIFGSPLVFTWKAAVVAALFHAGP
                     LLIKSSRAALESVDRSYEKAARNLGATEWRVFWRVSLPLAWRSILAAVVLAFARALGD
                     FGITIMIAGNIPGRTQTVAVAIYDAVESGNGMLARTLVLVISAVALLVLSIANRLGAK
                     RFGSTYTPV"
     gene            16582..>17853
                     /locus_tag="VFQ79_25355"
     CDS             16582..>17853
                     /locus_tag="VFQ79_25355"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0143074.1"
                     /translation="MPNGLNRRRFVNAAACASIVPRLFSASRKRPNILFAIADDQSWL
                     HAGAYGDPVVKTPAFDRIAERGVLFTNAFSGSPGCAPSRAAILTGRSPWQLEEAGTHA
                     SLFPKKFQVYPDILAAAGYHTGFTGKGAGPCNWKDAGWPHNPAGPEYNRRKAVQTSNA
                     ISAIDYAANFEEFLQKKPAESPFCFWYGAQEPHRPYRTGSGLESGKRLQDVVVPPFLP
                     DVSEVRGDMLDYYLEIEHFDRQLGKILGILEKSGELENTFLVATADNGMSFPRAKATM
                     YEYGIHLPLAVSWPARFAGGRKIDDLISFMDFAPTFLEAAGVGPNAVMTGRSILNVLD
                     SKQSGRVDASRDRIFSGRERHSHSRFDNLGYPARAIRTHEYLYIRNFKPDRWPAGDPE
                     GYFDIDACPSKDYLLKHRESEGVKRLFELTCG"
BASE COUNT         3535 a         5501 c         5166 g         3651 t
ORIGIN      
        1 tggcggtcgt ttttgccgga aatcatcctg acgccgccga gcgcctgctc gccgcggtcc
       61 gttatcaggc caacctgacc tggaacgaag ccccggcggc ccagccggaa aacatcggaa
      121 ccctcatcct ccacatcatg cttttgatcg gcctgctcct ggcggtctca gtcgtgttgg
      181 gttttgcgtt cggcgggctt cgcgtgttcc ggcatcgatg gcttggcagc aaaaatgctg
      241 atgaagcgat gatcacgctt cacctgggag accgttaagt tctatatttt tgttaacaaa
      301 actcagtgga atcgaaggct tcaggtgaga tatagagttc ggccggactg cctgtgaaac
      361 tgttagctat ctaatctaaa ttgcagataa caaataagtt gataatgaaa atcgctgaag
      421 gtgtttttta cttgacttcc cgccccgtag gtcataatat ccaatcaaac cagattttga
      481 tggttgcgtt ccgtcgattt ctgattctcc cattgaacat cagccctgtt acggcagggt
      541 cgtgactgat gctcacccct accgggtgag catcgtcgca tttgccctca actaaaacct
      601 ccgttggcta tactcggaat atatttatgg cagacagcct cttacaggta atcccactcg
      661 gcggcctcgg cgagttcggg atgaattgca tggccttgcg ctacggcgac gacattatcg
      721 tgatcgacgc cggcatgatg ttcccggacg cagaactcct cggggtggac atcgttacac
      781 ccgatttcac ttatctggtc gaaaaccggg agcatgttcg tgcggttatc ctcacccacg
      841 gccatgaaga tcacatcggc ggagttccct tccttctcgc cgaactgaat gtccccatct
      901 atgggaccgc gttcaccctg gctctcgtcg caaggcgcct cgaggaacat gaagttcagg
      961 ataccgtgcg gctcaatcgc gtaaagccgg gcgaacgcgt cacaatcggt gcgttcgaga
     1021 ttgaattcat caacgtaacg cattccatcg taagcgccct tgctctcgcg attcgcaccc
     1081 ccctgggagt ggtgattcat accggggact tcaaagttga cccaaccccg accgataatc
     1141 tgctgttcga tctgcacacg tttgcccatt acggaaagca gggcgtcctg ctgctgctgt
     1201 cggattcaac aaacgtcgac aggcccggct acacggaaag tgagctggcg gttcgtcccc
     1261 gcctggaaga catcttgacc cgggcggaga aacgggtggt gatcgcctgc ttcagctcgt
     1321 cgattcaccg gctccagcag attctggacc tttccgccca ggccggccgg aaagtggcct
     1381 tccttgggcg cagcatgctg accacgacgg agatcgctca tcatctcggg ttgctgagca
     1441 taccggatgg gattctcctg cgtccgcagg acattaacca gacagaccgc agcaaggtaa
     1501 ccgttgtcat ctccggcacg cagggcgagc cgatgtcgtc gctttcccgg gtggcggtgg
     1561 atagtcacaa gcacttgtcg atccagcctc aggatacggt tgtgctgagc tcgcgcatca
     1621 ttcccggtaa tgaaaaggcg atttaccgga tgatcaacca tctggcccgg cggggggcgg
     1681 acctggtgta tggctcgatg aacccgccga tccatgtgtc cggccatgcc agcgcggagg
     1741 agttgaaact gatactgaac ctggtccggc cgcgctattt tgtgcccatt catggagagt
     1801 accgccagct tgcaaagcat gcatcgctcg cccggcacct gctgcaccag gggatggagc
     1861 gttccttcat tctcgaaacg ggcgaaacgc tcgaaattga ccgccgcggt gcgcgcaagg
     1921 gtgaaaaagt tacggtaggc cgcgtttgca tcgattccgg ctcggtcgat gaggtggttg
     1981 aggatatcgt gatccgcgac cgccgtcacc tttccgagga cgggatcgtc ctgccgatca
     2041 tcgccattga caaaaacacc ggcaagcccg aggggctgcc tgaaatcgtc agccgcggat
     2101 tcgtagcgcg ggagaatggc tcggaaatat tgcaggacgc ccgccaggtt gtcgcgcgca
     2161 cgctcgaggt ctcgagcccc gaagagcgcg ccgactgggg cgtaatgcag gagaagatcc
     2221 gggccgacct gaagcgtttc ctgaacaaac agacctcccg ccgtccgctg atcatgcctg
     2281 tgatcctgga agtgtagccg gccgtagacc ttcctattga cgctcgatat gaagcgcagt
     2341 atacttctat cgattttctg taggagagta ctttggccaa aaattcagaa ctacttcagg
     2401 gcacattgga tttgttgatc ctaaaagctc ttgctttggg ctccaaccat ggtctcggcg
     2461 tctcccggcg aatcgcccag atcacacagg gaaccttcga cgtcaagccc ggctctcttt
     2521 tccctgcctt gcatcgcctc gaggaaaaag gttggctggt ggccgaatgg ggacagtcgg
     2581 agaacaaccg gcgagcaaaa tactaccgct tgacacaggc gggccgccgc cggctggaca
     2641 ccgaagtgaa cgcgtggcgg cgcacctcgg gcgccattac gcgggccatt gaagcgacgt
     2701 cttgaggccg tgcgccatgc agatgtttga gcaactcaag gccctggtac gaaatgttac
     2761 cgggaaggcg agagtggaac aggatctcga cgaggaggtc cgcgcgtatc tcgacatgac
     2821 catcgacgag aaggtttcgc gtggcgtgag ccgggacaaa gctttgcggg aagcctggat
     2881 cgagctgggc ggggccgagc aactgaaaga ggaggtacgt gccgcgaaac tcggggcttc
     2941 cctcgagaaa acatggcggg actttcgctt tgctctccgg ctgtttgccc ggaaccccgg
     3001 cttcacagcc gtcatagtac tgtcgctggg gctttgcatt ggcgccaaca ccgcgatttt
     3061 ctccctcgtc aatgcactct tgctgaagac gctgccggtg aaagagccgg agcggctcgt
     3121 cctctatagc gacggacggg cccggggatt catctcggga caaagcggcc gctggaacat
     3181 cttctcctat cccctctacc ggcgctttcg ggaacccgga acgtctttcg aagatgtggc
     3241 tgcgttccgg acgcaattgg accggttgag ccttcgcgct cacggaacgg gcggcggtga
     3301 aggcgcgcag ttgacttggg gccgtttggt ttccggcaac tacttttcgg tgcttggagt
     3361 aaaggcggcc ctcgggcgtg gtctcactgc ggacgacgag cgcctggctg ccgtgcctgt
     3421 ggccgtcctg gccttcgact attggaaacg gcagtatgaa tcctccccct ccgtggtagg
     3481 ccaggtcatc gacataaacg gcgtgccggt gacgattgtg ggtgtggcgc cggccgagtt
     3541 ttttggggaa agcgtggaaa gccaactggc ggatgtctgg ctgcccctga cccttcagcc
     3601 gcgcatcatg cagcggcagt ccgctttaga agatgcggaa gcaaactggc tcaacctgat
     3661 cggacggttg agaccgggta gcaccatggt gcaggcacag gcggaggtca gtgcccgatt
     3721 tcagcagttc ctcatcgaac tggccggtgc gaacctcaca ggagaacaac agcagcagct
     3781 ccagcgtaac cacgttgttc tgactccggg cgccaatggc atatccgcgt tgcgggtccg
     3841 gtactcacgt gctttgcggc ttctcatggg agtagtcgga tttgtattgt tgattgcctg
     3901 tgcgaatgtg gcgaacctgc tgctttcccg cgctgcggcg cgtgagaaag aaatgtccat
     3961 gcgtctggcg ctcggcgccg gacgtggccg cctgctccgg cagcttttga ctgagagcgt
     4021 cctgctaact tgcatgggcg gagcgttggg tctgttgctg tcgggctgga tggcccgtgc
     4081 gttggtcgcg ggcgtgtcca ctggagatcg aacggttcct ctgaatctct ctccagacgc
     4141 ctccgtgctc ggcttcacgg cgggcgtttg catactggtc gtgatcctgt tcggcttggc
     4201 gccggccctg cgaacatcac gagtcgacct gatgactgcc ctgaaagctt ccggagccgc
     4261 tggaagtggg aggtcgcgcc gtgggctcgc ccgaggattc gtgatcgcgc aggtcgcact
     4321 ctcgctgccg cttctcgccg gcgcggcgct tcttgtacag actttgcgag agcttcgcac
     4381 gcaggacttc ggtttctcgc atgaaaacgt gctcgaagtc gggattgatc cgggtatcgc
     4441 cgggtataag cccgatcagg taggaacgct ataccgggct gtgctggatc gtgtgcactc
     4501 tgttcccagt gtgcgcatag cgagcctctc ccagtacagt ccaatgagcg gggacaactg
     4561 gtccgggcag gtccaggttg aaggctacac cccatctcgc ggggaaaacg cctactgcca
     4621 gtgggtgtgg gtaggacccc ggtatgcgga aaccttgggg atgacgctgg tgctcggccg
     4681 tgacctggac aagcgcgata ccgaaaattc gcccaaggtg gcggtggtca acgagagttt
     4741 cgcccgccgg tacctggctg accagaatcc gatcggccgt cacttctctc tcaaagacac
     4801 catcgagatc gtgggtgtcg tgaaggactt caaattcaac gacccccggc aggagatctg
     4861 gccggtagcc ttcatgcctc tggtccaatc ctcatttacc ccggcacgct tcgcccggta
     4921 tctcgaagtt cgagcaggcg gtgaccctct aagcatagcc gccaatattc gggaagccgt
     4981 gaaagaggtt gcaccgggtc tgccagtcac gggcatgaaa acactgagcc aacaaatcga
     5041 tacagcgctt ggccgggaag atctgatcgc aggtgtctca agctctttcg cggtgctggc
     5101 cctactgctg gcatgccttg gattgtatgg ggtagtgagc tattctgtcg ccggacgaac
     5161 caaggaaatc ggcgtgcgta tggctctggg agcgcgccgg ccgaacattc tgacaatggt
     5221 cttgcgcgaa gcgttgctgt tggcgctgat cggcgccggg atcggcctgg cgacgttcct
     5281 tgccgttgca gaagccgttt ccagccaact ctacgggttg aagcccaccg atccttacat
     5341 tatggccggc gcgtcgctgc tgttaatcgc ggcggcgggc ttggccggat ttataccggc
     5401 tcacagagct tcacgcgttg accccatgac aacgttacga gcggaatgaa gagccgacat
     5461 tccgtgacaa ttcgttgaca ctccccgcga aagtgtaata cgctaccttg cactcggtag
     5521 ggaatcacca tttcgatctg ggacttcaca atgacacttc ttcgttccgt tgttctcttc
     5581 gtcgtagttt cgacggccct gtacgcacag caaaccacgg gcgctattcg tggcgccgtt
     5641 acggacccga gcggcgcatc ggtggcggat gtgaccatca ctgccacaaa taatgatacg
     5701 ggcgcgacac aaacaacgaa aaccggtgcg ggcgggaact acgttcttcc actcctgccg
     5761 ccggggaggt actctgtttc cgcggagagc caagggttca ccaaagcgct ccgcagcaat
     5821 gttctggttc gcatcacgga gaccgaggtg gtggaatttt ccctccaact cggctcggtg
     5881 gctgaatcgg ttacggtaga cgatactgtc tcgctggtgc aaagcgagac ggcgacggag
     5941 ggccgcgtta ttgagcggca gacgatcagc gctctacctc tggcgacacg gaacttcacc
     6001 caattgctcg gcttgacggc tggtgtggtc acagatcccc cgaacgcgga aaacgtcggc
     6061 ttcgggacgc aaaacccgag cgtgaacgga tcgcggcgcg gcagcaataa ctacctgctc
     6121 gacggcaacg tcaacaacaa tccgatgaac aacgacgtgc tcggggtggg aattccaagc
     6181 gtcgatttca ttcaggagtt caaggttctc accagcatgg caggcgccga atacgggcgg
     6241 aacgcaggct ctgtcgtaaa cgtcgtcacg cgatccggca ccaaccagtt tcacggcaac
     6301 ctgttcgagt ttttgcgtaa cgacaagttc aatgcgcggc cgtttttcgc ggctcgccgc
     6361 ggtcagaatg tccagaacca gtttggcgcg accatcgggg gcccggtctg gattcctggc
     6421 gtttaccagg gccgcgaccg caccttcttc ttcttcggct gggaatcgat ccggcagcgc
     6481 aacacgaatt cgaccgccgc cattgtgatc ggccgcgttc cgactccagc ggagcggcgc
     6541 ggccagttcg acacgccggt tcgcgatcca ttgacgaaca atccgttccc tggcggtgtg
     6601 atccccgagg ctcgcatcaa cccaacctcg gcccaactcc tcgagcgttt cgtcccactg
     6661 ccgaatacga cgaacccaac cagtaatttc cttcagcagt tcggcacgcc cttcgatgcc
     6721 aaccagtaca cgatccgcgt cgaccacgaa atcggcccgc gcgaccggtt catggcgcgc
     6781 tacttcgatt cgaactcgta ctccttcacc gcctccggcc ggctgcccgg cttcggccgc
     6841 gtctccgatc cgattaaccg ccaggtcgca gcgtcggaga cacatatctt ccggccgaat
     6901 ctcttgaacg agatccgctt cggcttctac aagttccctt cgcccgggac cgatgtgcag
     6961 cggatcgatc cacgaagcgt cggcatcgag ccgatgaacg acgtccccgg gctgccggga
     7021 atgctggtga ccggcttcct cgggtacggc tacaccggca acgacttcaa ggacgagcag
     7081 aacaattttc agttcgcgaa tgtgctgtcc tggatttccg gcaaccactc catgcggatt
     7141 ggcggtgagt accggtacaa catgatgcta tcgtccggtg tgccgttcca gggccgcttc
     7201 gcttttaacg gccagtacac gacaaacgca ttcgcggact tcctgctggg ggcgccgaac
     7261 caggtgattg tggccaatgg accggcggtg atcgacatgc gcgcctccaa ctacgcattc
     7321 ttcttccagg atgactggaa ggccacccgg cggctcacct tgaacgtggg gctgcgctac
     7381 gaatacgaca cacccccgac cgacgtggcg ctgggccgtc tgttgaactt ctatccggag
     7441 cgctatcgtg ggcccggcgt tgagtccggc ctggtgatcg gcggtgaaag tccgggggtc
     7501 ccgccgtcca cggtctttcc ggaccgcacg aatttcggtc cgcggatcgg ttttgcctac
     7561 gcgctgggtt cgaactccca cactgtgctt cgcggcggct acggcatctt ctacgacagc
     7621 cgctccggac aggtcactca gcaaaagctc ttcgagccgc cctattcggc gaaccagact
     7681 gtcgtcatgc gtccggccga cgcgccgcgt ggcttcgtct tccccgcccc gatcgacctc
     7741 tcgcgcccaa ccgagacagt ccctggcggc acgctgtcga tccggcccat cgagcccaac
     7801 acgcgtatgg actatgtcca gcagtggaac ctcggaatcc agcaccagtt cgaaaacgga
     7861 attctcgtcg ccgcttccta tgttggaacc cgcggcctgc agttgttccg cgccacgaat
     7921 atcaactatc cgcggctggt gggcgacacg ttcgtccgcc cctatgatgg ctttacgacc
     7981 atcgtgcagt cgcaagccgg ggccacgtcc gattatcact cgggccagct aaccgtccag
     8041 aagcgtttct cccgcggggt atccgtcctg ggggcataca ccttcggaaa ggcgctggac
     8101 gatagtacct ccggcgtccg ctacttcact gcggccactg gcgaccccgg agatctcgct
     8161 gcaagccgtg gcccggctga ttttgaccgc acacatcgcc tggtcgtcag ctacaacctc
     8221 gaccttccca atccatttgg acgtggtgtg cagggtgtga agcgagcgct gctttcagga
     8281 tgggaactct ccggtgtcac aaccatacag tcaggcacac cgttctcggt taccaatgcg
     8341 gcgagcaatc ttgaccgtga cgggcaggcc ggcgcgcctg gcactggcgg acgggccgac
     8401 cgggtcggcg acatttcaca aacgaccgag ggcgacgtcc gctctcggct cgatcactat
     8461 ctcaacccca ccgcatttgc gcctgcgccc cgctcacgct acggcaacct tggccgcaat
     8521 acgattcgag gtccgggcgc tcatctctgg gacgcacgaa tgaccaagct gacaacgatt
     8581 cgtgaaggac tcaacctgcg gttccttgca gagttcttca atatctggaa ccatgcggct
     8641 ttcggaaacc cgggaagcgt gctgggcact cctgctttcg gcaccattcg ctccactttg
     8701 tcgaacgccc gtattataca gtttggactg aagcttgaat tctagaaacc atgctcgact
     8761 aacccgctgc gcgctggcag gcggcgctgc cggcctgctg ctattcgcgg cgcacttcac
     8821 gcaaagggag gccgcgggcc aggcggccgt tcctctctcc aagccaggcc aggaggatct
     8881 ctctttctgg gtgaaccaca acggactttt gcagttcgaa gccgaaggcg aggtcgggtt
     8941 cggcgacatc gcccgcattc tgctccgccg ccgcaccaac ggcgaagcaa tccgcgaatt
     9001 gcgtgaccgc ttcaccttcc gggtgcagga tgcggcggcg ctgaccacgc cggatgtttt
     9061 tcgcgccgag tatagcagcg gtaacctgat gtggcgcgcc gctccgcagt cccgtttcgt
     9121 ggcagggtct tcacgaaccg tcaacattcc ggtcgtggtc gtcaatcgcg actcacagcc
     9181 catcacgatt gacgcgatgt atgcaggcgc atcgatggaa agcgctgccc ggaatatcgc
     9241 gattgctccg ggcggcaaca ccgctatcct gctacgcaca gtggagacca gcatcggtcc
     9301 gtcggccggc aagctgactc tccgtcacgg caccggagaa atcaccactg ctatccattt
     9361 cgatgttcgc ccactggtgc ctctgcgcgt ccgcctgctc gatgaacgag gccagcccgc
     9421 catcgcgcgt gtttatatca ccggctctga cgggctttct tatgtgccta cgggatcctc
     9481 cgctcgcatt acggcaatga gcgccgaata cttctttcac gcggacagcg atttcgagat
     9541 ccaactcccc gccggcgaaa cgctgctgga ggccactcgc ggccccgaat atcgcctcac
     9601 cgccgaacgc gttcatcttc agccgggaac tcccgccgaa gccactttgc aattggcccg
     9661 gtgggcgcac cccgctcaga aaggctggtg gtccggcgat gtgcacatcc acgccaacta
     9721 cacctcaccc catcaccagg tgattgaacc gcgcgatgtc cggctacagg ttgcgggtga
     9781 ggatttgaat tacgcgaacc tgatggtggc caactccagc ggcgcgttca ttcatgaccg
     9841 ccagtatttc gaagcgaaac cccaccgcct ctccacgccc gaacacttcc tctactggaa
     9901 cgaagagaac cgttccagcg cgtacgggca catgtgcttt ctcggcttga agcacctggt
     9961 ggagcctttc tataacggct ttcgcaacac gccccattgg gaggattatc cggccaatta
    10021 cacgctttct caacaggtct tcgaccaggg tggcgcggtg agctatgccc accccggcat
    10081 ggcgccgaac ttcgagtcag cgagcataaa ggaacttcca gtggacctcg cactaggcca
    10141 gccgaccgcc atggatgtgc tcagcaacaa cgacgaagcc gcgacgacgg agatgtggta
    10201 ccgtctgctg aactgcggct tccgggtagc gatctcggcc gggacggatg cgttcacgaa
    10261 cgtcgtcgat cactatattg ggggcgggaa ccgcgtctat gtgcaatcgg gcccgcgatt
    10321 tgactatgaa gcgtggctcg aaggcttccg ccgcgggcgg tccttcgctt caaacgggcc
    10381 gataaccact cttgtggtga acgaccggtc cccgggcgac gaaattcttc tcgaccgcga
    10441 agaagaagtt gtcgtgcgtg gtaccgtctc ctctcaggtc cctctggagc ggatcgatgt
    10501 gatcgtgaac ggcgcacccg tccacacgat ttcaccggcc ggcaaagaga ccgtggactt
    10561 cacgcatcgc atcgtcatta aagagagttc gtggatcgcg ctccgcgcgc tcggtccgcg
    10621 tcaccggctg atcctgaacg atacccaggc ctacgcgcac acgagccccg tttacgtgac
    10681 ggtcgccggc cgtcctgttc acgtcgccga cgacgtgcgc ttctatcggg aatgggttga
    10741 acgcctcatc gcccgcacgg agacgcgtgg ccgtttcgcc actcccgagc ggaaagctga
    10801 agtcctcgca ctgtttagaa aagggctgga ttggtaccgc cgcgccgaag cgggaaaacg
    10861 cgaacgctct tactgagccg cgcgagcaag cggtcttgac aggtcaattc gctaaactgt
    10921 acactgtatt cgcacagtga acagatggct aaaccaaagc ctgcgatacg aatcgatctg
    10981 aattcgcctg tcccgaacta ccggcagatt gtggattccc ttcgtactca cctggttgac
    11041 ggcgcgcttg caccggggga tgtgcttcca tcggtccgtc gtctggcgat ggaacttggc
    11101 atcacattca ataccgtcgc ccaggcctac cgccagcttg ccgaagaagg ctggctggag
    11161 ttgaaacacg gacggcgcgc catagtgatt gatcgccatc ttccttccag cgccagccgc
    11221 gaaaaggtca ccggattccg gttgcgtatc cgccagatgg ttgcgcagat gcgcgccgaa
    11281 ggcttatctc cccgggacat tgccagagaa ctgcacctcc ttgccgaaaa gctggaagca
    11341 tgacgatgat acgactttat ttgttgttgc cccttgccgc taccctcgcg accctgcatg
    11401 tacttccctt cctgccgcat cgcgatcgtc tcttcggcgt cgccgttcca ccagaagttc
    11461 gatacggcag cgaagaccgg caagctttgc ggcgctatga acttcacttg ctgccctgga
    11521 ccgctacggc cttctttgtg tcattatggc tgccgttatc ctgggcggcc gtctggatgt
    11581 ccgccgcctc ccttctgccg ttaattgccg ccggccggat cttctggcga acgcgcggcg
    11641 agattcagca tctcgcctta cccgcgtctt ccacacgcca ggcgcagttg acggatacgg
    11701 atagccacct ccagcggcag acgctgctgt ttcttatccc gatggccatc ctggccggga
    11761 cagcgcttta ccttcatctt cactgggatc aaattccgcc gcgcttccct gtacattggg
    11821 gaacggatgg gactcctaac ggctggtcca ctcggagttt cgccggtgtc tacgggccgc
    11881 tgcttatcgg tgccatcatc gttctgttta ttctgggcat ctccgcggtg atctcatggg
    11941 gttctcgccg aagcacccgg ccatccgccg ctcaaccgat tttagttgtc gtcgcctgca
    12001 ttatcgcggc aggttgttca ctggccggac ttttgccgct tcacgtcaca cccgtatggg
    12061 gactggtggt ttttaacgcc gtttccttcg ggctcatcgc cgtggcgatc tggttttccg
    12121 tccggcgccg cggggaaacc gctccagagt ctggagaagt gacaccggag aactgctggc
    12181 attgttgggg acaattctat cacaaccccc aggaccccgc gctgtttgtt gaaaagcgca
    12241 tcggcttggg atggaccttc aatttcggta accgtttgtc atgggtcgtt cttgccctga
    12301 tgaacctgat tccagccgga ctgatattcc tcatattaca gttcacgaaa agctgaagta
    12361 cgattgacat tgacatgata atccgggtga tattgccggt actcctgtgc tccgcttgct
    12421 ttgcggcgaa cattcccgct ggcgccgagc ttcacgttcg cctccagaac aaggtcgcca
    12481 gcaacacctc caaacccggt gatgccgttt cctccattgt catcgctcca gtcgtggtag
    12541 atggcgccgt cgtgctgcac tccggagtcc ggctgcacgg ggaggtgaaa tccgccaaga
    12601 cttccaaagg accggaagat cgcgccatgc tggagatcgt attcaaccgg atggaggatt
    12661 cgacggggca gcgcgtggag attcaatccc gcgtttccga ggtggacaac gcgcgggaga
    12721 gcgtggacga acaggggcgc attaacggca ttgttgcctc tcagaccatt tcggcgcgta
    12781 tggatcaggg gatcggcaaa gtcgctcaaa ggagcggcgg cctcgcggat ttcctgggac
    12841 tcgtcaaggg agcggtactg aaagaagccg acgcggaaat cgtctatgaa cctggcgtgg
    12901 aaatgaatgt gaagctaacg gcaccgatcg aatggaagga taccggcgcg gaccccaatc
    12961 tcgtgccggt cgccgacccg gacgcgttaa accgcttagt gaactctcaa ccctatcaga
    13021 ccagggcgga aaaacctccc aagccgtccg atatcacgaa tctgatgctc atcggaacgg
    13081 aggaggagat ccgggaagca ttcaaagcag caggatggtt caccgcggcc gttctcaaca
    13141 gtctctccgc gttggagacg tttcgcgcca ttgccgaatc tcgcggctac agtgaagcgc
    13201 cgatgtccat tctgctgctg gacgaccaga aggccgccat ggattttcag aagcagaaca
    13261 acacttttgc catgcggcac catcttcgcg tgtggaagcg tcccgacacg tttcagggca
    13321 agccggtatg ggttgtggca gcgacgcacg acatcggaat tgagctgtcg ccggagcagc
    13381 gcaccttcat acacaaaatc gattcgaata tcgaccgcga gcgtgcaaag gtggtcagcg
    13441 atcttctctt tacaggaaag gtcagctcgc tcgcgctggt ccagcgaccg gatgttccgc
    13501 gcgagacgaa aaacgcaacc ggggacacgg tggttacgga cggcgcgatg gcggtcctga
    13561 tcttctgaag ttaaccgtcc acgcgctgga acatactgat acccgccact cccgccgcgc
    13621 ccgcctggat gcacagcggc gtgttcaact cgttcactcc accgagagcg agtaccggaa
    13681 gagagctcgc ctggacggct tcgcggagct tgtcaaggcc ctgcggcgca ccgtagccgg
    13741 ctttggacgg cgtaaaaaaa accggactga aaacaacaaa atcggcccct tccgcacgct
    13801 ggatctcttc gctgctgtga caggacacgc caatgagaaa cggctttgga gcaatctgcc
    13861 gcagcctcgc cggagggatt ggatcggaca tcaggtgcac gccgtgagct ccgcaggcaa
    13921 gggcgacatc cgttctcgaa ttcaccagga tcctggtgcc acttggattc ggaaacgcaa
    13981 gcgccgcccg caccaggtcg gcgagtgccc gtgccgtaag gtctttctcg cgaatctgga
    14041 tcatgtcgat tccagaagct aaagcggaac cgatgttggc gagcagagcc tccacgccac
    14101 ccaattgctt gcggtccgta atgtaatacc ggatcacttc aggccggctc gtaatagcgc
    14161 tcgccggcgc aggacttgca tagcgttttc ccgttacgca ccacttcgcg cttgaaattg
    14221 atcccttcgc cgcattcctc acacaccact cgcggcgctt tgtatcccgg catttcttcc
    14281 gggccgagcg tcacgcgcac ccactgcttg tcaaagagat cctcatcggg catttcgcga
    14341 taggcgcgca tctgctgctg gtttttatcc gcaatatcgg gatacagttg ctttgcgcgt
    14401 tccttcgacg attctttggc gaccacccgc accgccttcc cggcctgaag atcgcaaaag
    14461 gtagccgcca cttttccgaa gtcgcggaat ttcagggccc gcttgcccag gcggcacccc
    14521 gttacgactc caatggcatc ggtggcgcac cggtcgattt cgacaaacgt gatcagacgc
    14581 ttgcgatctt tgccggccgg gtcctccaaa ccaagcaggt tcacgccata gacggccatg
    14641 cgcaacccga gaatctggcc ggcgcaaata tggccgtggg ccagtctggc aagttcaacg
    14701 tattcgtcaa aagctttcat tcgtctcctg ccggggcgcg gtatgacggt tcaaagcacg
    14761 cgtagtcctt caaccggaaa ctcgatcacc caatctttat tatgcttgtg tccttcgaat
    14821 tgggatcggg gaacctccgc cgcgagatcg cccgagaact cgagccggac cgcctgaggc
    14881 ttctccacgg cacgcaagag attcgcacgc acctggttcg gaccaggctt tccgtcagcg
    14941 ggagataccg taagctgctc ggggtggatg cacagccaga cgcgatcgcc tttaaacctg
    15001 ccggggaagt agggtcccgt aatctcgaac tcctcgtatc gcaggcggct ggtgtttcta
    15061 cccggatcga gtgcccggat ttccaccggg agaagattgt agatgccgaa cagccgcgct
    15121 acttcgatgc cggcgggctg ctcgacgacc ttccgtggag gtccggtctg gactaaccgg
    15181 ccatcgcgca gcaccagcat ttcctcgccg agcgcaaagc attcctccag gtcgtgcgtc
    15241 accaccagta ttgggattcc gaactcggcg cgtatttgcc tcaacaccgt gtatagctcg
    15301 actcgcagcg gggcatccag ccctcgcgcg ggctcatcga gcagcaacag cttcggactg
    15361 ccgatcatcg cccgcgcaat ggagcagcgc tgtttttgcc cgcccgagac ctcatgcggt
    15421 ctgcgtcccg caacctccac gatatgaaag cgctccagca tttcggtgac gcttcgatgc
    15481 cgttccagcc tcggaatatt tttggcggca aaatcgacgt tgccccgcag cgacatgtgc
    15541 ggaaatagag catagttctg aaacacgtat ccacaacgcc gcgcccgcgg cgggatgttg
    15601 acgcgcgccg cgccgtcaaa cagaatggca tcgtcgagca ggatgcggcc ttcatcaggt
    15661 tgtacgaatc ccgctataca gtcgagaatg agcgtctttc ccgcgccact cgggccgaag
    15721 agcaccgtga tgcccgcgcc taattccaac tggacatcga gggagaacgc gctcgactcc
    15781 ggtcccgccg cgaaattttt gcgaatacgg gcccggatca tacaggcgtg taggtggagc
    15841 cgaagcgctt cgctccgagc cggtttgcta tggaaagcac aaggagtgca accgccgaga
    15901 tcacaagcac cagggtccgc gccagcatcc cattgccgga ttccacggca tcgtagatgg
    15961 caaccgcaac ggtctgcgta cgcccgggaa tgttgcccgc gatcataatg gtgattccga
    16021 aatcgccgag cgcccgtgcg aacgccagca ccaccgcggc cagaatcgag cgccaggcga
    16081 gcggcaggct cacccgccag aacacgcgcc attccgtcgc gcccaggttg cgcgccgcct
    16141 tctcgtagct gcggtcgacg ctttcgagcg ccgcgcgcga cgatttaatc agcaatggtc
    16201 cggcatgaaa cagcgccgcc accaccgctg ccttccacgt gaagaccaat ggggaaccga
    16261 agatgccttc gaacaacttg ccaaaaggac tttgccttcc cagaaccacg agcaggtaat
    16321 agccgagcac ggtgggcggc agcaccaacg gcagcgtcac cgccgcgtcg atcacttcct
    16381 tccagcggaa ttggcggttt gcgagcacgt aagccagggc aagcccgccg aagaacgcaa
    16441 tgacagtcga aagcgccgcg acacgaaggc tcaaccacag cggaaaccag tctatctgca
    16501 tccggaaatt cgattataac gatgagcagc gaagtcgccc acccgaagaa gaatagcacc
    16561 gggtgtaaca taggtcgagt catgccaaac ggcctcaacc ggcgccggtt cgtcaacgcc
    16621 gctgcgtgcg catcgatcgt cccacgcctt ttttccgctt cccgaaaacg cccgaacatc
    16681 ctgttcgcca ttgccgacga ccagtcgtgg ttgcacgccg gcgcttacgg cgacccggtc
    16741 gtgaagacac cggctttcga ccgcatcgcg gagcggggcg tgttgttcac gaatgcgttc
    16801 agcggctctc cgggctgcgc tccgtcgcgc gccgccatcc tgacgggccg cagcccctgg
    16861 caactggaag aagcgggcac gcatgccagc ctgttcccca aaaagtttca ggtttatccc
    16921 gatatcctgg cggccgccgg ataccacacc gggttcaccg gcaaaggagc cggcccctgc
    16981 aactggaagg atgcggggtg gccgcacaac cccgccgggc cggagtacaa tcgccggaaa
    17041 gcggttcaaa cttcgaatgc gatcagtgcc atcgactatg ccgccaactt cgaagagttt
    17101 ctccagaaga aaccggcgga aagccccttc tgcttttggt acggcgcgca ggagccgcac
    17161 cgcccctacc ggactgggtc cggtttggaa tccggcaaac ggctccagga tgttgtagtg
    17221 ccgcccttcc tgcccgacgt gagcgaagtc cgcggcgaca tgctcgatta ctatctcgaa
    17281 atcgaacact tcgaccggca actcggaaaa atactcggca ttctcgagaa atccggcgag
    17341 ttggagaata cgtttctggt cgccaccgcc gataacggca tgtcgtttcc gcgggcgaag
    17401 gccacgatgt atgagtacgg catacacctg ccgctggcgg tttcgtggcc cgcgcgtttt
    17461 gcaggcggcc gtaaaattga tgaccttatc agcttcatgg atttcgcgcc gacattcctc
    17521 gaagccgccg gcgtcgggcc aaacgcggta atgacgggcc gcagcatcct gaacgtactc
    17581 gattcgaagc aatcgggccg ggtggatgcc tcgagagacc gtattttttc gggccgggaa
    17641 cgccactcgc attcgcggtt cgataatctc ggttatccgg cgcgcgccat tcgcacacac
    17701 gaatatctgt acatccgcaa cttcaagccg gaccgctggc ctgccggcga tccggaaggc
    17761 tacttcgaca ttgatgcctg tccgtcaaaa gactaccttc tgaagcaccg cgaatcggaa
    17821 ggggtgaagc ggctgttcga actgacgtgc ggc
//
LOCUS       DASTMK010000615        28077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_255492, whole genome shotgun sequence.
ACCESSION   DASTMK010000615 DASTMK010000000
VERSION     DASTMK010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 28077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28077
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255492"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1853
                     /locus_tag="VFQ79_25360"
     CDS             159..1853
                     /locus_tag="VFQ79_25360"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEU0143075.1"
                     /translation="MRFLALLVLPALLGAQVPSKEDVLRAMRKAADFYRHKVSTHGGY
                     HFYYTADLSYGRSESAEGPTQVEVQREATPIVALAYLDAYEVTGDRTYLDYARDAAHA
                     LVKGQLCSGGWDYLIEFDPEKRKRYQYREDGDCGGAGRNVTTLDDNVTQAAIRVLMRV
                     DKALDFSDAAIHESARLALDRLIAAQYPVGAWPQRFSAPPDFSRFPVKRASYPETWSR
                     KWPGPEFQQHYTFNDNSIADAIDTMLEAARIYNEPRYRASAEKGGQFILLAQMPDPQP
                     AWAQQYDADMHPAWARVFEPPSVTGGESQGILRVLMTLYRETGDRKYLEPIPRALEYL
                     KRSFVPRADAEAFRRLPSGPVLARFYELKTNRPLYITKGTRLNVAGMGSRLVDGYEIS
                     YTPESVITHYGVLVSGNQLNVIEEDYRKLGAAAPKGVRRPDLLRGLSPWSERPFRMTE
                     APPPPNEVKELIASMDGRGAWTKMGTIGRADRLVFAFAAKDLVLRIGRGRSDGSAGDG
                     LNSQADIIPIQENMTVELFAGPQPPPVSIISSADFAGNLARLGRYLTASRQTRAGR"
     gene            1872..3221
                     /locus_tag="VFQ79_25365"
     CDS             1872..3221
                     /locus_tag="VFQ79_25365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143076.1"
                     /translation="MRRRDFFKSAAVLAAPRAEQFQLATFQSDITPSIGGPLFNNVTA
                     RKIVDPLQAHGVVLLGGGRPIVVAVLDWCEIRNDAYNLWRDVMAEAARTSRERVLLTC
                     VHQHDAPYVDTEAQRLFEAAKAPGRICDPGFNEHCARKVGAALQASLNSARRITHIGT
                     GQAKVERVASNRRYVRPDGGVSFGRTSATKDPALRALPEGTIDPWLKTISFWEEDRPV
                     AALSCYSTHPMSYYGQGDVSADFVGMARARRQKDTPGTAQIYLTGCSGDTIAGKYNDG
                     SPANRPVLAARMYQGMVGAWKATKRRPLKNVRFRSVPMHLAPRATPGFSPAEMRKTLA
                     DESKPYLARAEAALGLSWRKRVDAGEAVDVPAVDFGGAHLVLLPAEAFVQYQLWAQEL
                     RPDSFVMTAGFSESAPGYIPTAQAAAEGYDDHYSWVAFPECENTMRNALRDALRRYD"
     gene            3222..4757
                     /locus_tag="VFQ79_25370"
     CDS             3222..4757
                     /locus_tag="VFQ79_25370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEU0143077.1"
                     /translation="MNRRRFVTTLGGIPAAAGAFEERRPNILLIFSDDHAYQAISAYD
                     GRLNHTPNIDRLAREGVRFDNALVTNSICAPSRAVVLTGKYSHLNGVLDNRQTFDGAQ
                     QTFPKILRQAGYQTALFGKWHLKSNPTGFDVWEVLPGQGSYYNPDFLTPDGRKRREGY
                     VTELITGLTLDWLENGRDKTKPFMLMCQHKAPHRNWMPAPSKLYLYDGVTFPEPPNLF
                     DNYEHRATPARKQAMEIDRHMTLMADLKILPAGGTPPQDADYRRYLGEYGRMNEAQKR
                     MWDAAYRPRNEAFARANPQGRDLVRYKYQRYLQDYLRCIASVDDSVGRILNWLDATGE
                     AENTLVVYASDQGFYLGEHGWFDKRWIYEESIRTPMLARFPGKARAGYVCESMVANLD
                     LPETFLDAAGLRVPADMQGHSFLRLLRGETPSGWRQSFYYHYYERGEHDVAPHYGVRT
                     DRYTLAHYYDTGEWELFDRMKDPHQMRSVYAEASYADVVKKLKAELTRLQRELRVPDS
                     KPI"
     gene            4815..5240
                     /locus_tag="VFQ79_25375"
     CDS             4815..5240
                     /locus_tag="VFQ79_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU0143078.1"
                     /translation="MNISVKGEYALLALFDLASQRPGEPVKIADIARRQKIPQKFLEL
                     ILATLKQGGFVESRRGAEGGYLLARPADSITIGELLRFIEAPRSNRSKGKRKSDSPFA
                     SMWRQVDEAISSVVDQTTFGELTRAWKENQMNYVPNWEI"
     gene            5243..6217
                     /gene="cysK"
                     /locus_tag="VFQ79_25380"
     CDS             5243..6217
                     /gene="cysK"
                     /locus_tag="VFQ79_25380"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU0143079.1"
                     /translation="MQPTTGKSFFEDNSFSIGRTPLVKLNRITDGAPVTMLAKIEGRN
                     PAYSVKCRIGAAMIWDAEKRGILTPGKELIEPTSGNTGIALAFVAAARGYPITLTMPE
                     TMSIERRKVLKAFGANLVLTEGSTGMKGAIAKAEEFVVSNPNRYVLLQQFKNPANPDI
                     HFQTTGPEIWEDTGGQIDVLVSGVGTGGTITGVSRYIKHKLGKPLVSVAVEPSNSPVI
                     TQAKKGEPLKPGPHKIQGLGAGFIPDTLDLSVVDLVEQVTNEESIEMARRLAREEGIL
                     SGISCGAAVVAAVRLARQPEFHNKTMVVILPDSGERYLSSVLFDGLVD"
     gene            6408..7316
                     /locus_tag="VFQ79_25385"
     CDS             6408..7316
                     /locus_tag="VFQ79_25385"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU0143080.1"
                     /translation="MPQSPNIYIGPAGWSYPHWNGAVYPEARPRGFHALHFLSEFTDL
                     VEINTSFYQPLRPEITSVWLKKVDHNPKFLFTAMLNRRFTHERSLEPAEVVANKRGLW
                     PLLKEGKLGCLLMQFPWAFRFTEENRAFLIKLRRTFHEFPLAAEMRHSSWMADEALGV
                     FVDYRIGFCNIDIPQHVKTMPPTAFLTSGIAYVRLHGRNAGLGIQNFDAAPQNSQDCD
                     YLYSAEELSEWKPRIERIARLAERTFVVTNNHGGGRAIVNALMLEELFGKTPAAAPAG
                     LRRLYTSQLKALPLETPLQNTLFEAA"
     gene            7377..8861
                     /locus_tag="VFQ79_25390"
     CDS             7377..8861
                     /locus_tag="VFQ79_25390"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0143081.1"
                     /translation="MRKRAAPLDMSPEEFRRLGYTFIDKVADLLAEIRSRPVTPGESP
                     TSVRNALGNAPLPQEGFAAEDALARAWELLSTHSLLNGHPRFSGYVTSPAAPVGMLGD
                     LMAAAVNPNAGAWILSPMATEIESQTVRWIAEFLGYPADCGGLLVSGGNMANFIGFLA
                     ARRAKSARDLRAEGVEPGKLAAYVSTETHTWIHKAADLFGLGTNSIRWIPIDDEFRMR
                     TGDLVDSIENDRAAGFTPFFLSGSAGTVSTGAVDPLEELAEIARRYDLWFHVDGAYGA
                     PAAALPGASAHLKALSQADSLAVDPHKWLYAPLEAGCALVRDRRRLFDTFDYSPPYYR
                     FDANEGEKPLNYYSYGPQNSRGFRALKVWLGLMTAGREGLIQMIGDDMELARTLFREI
                     RNHPELEAFTQSLSITTFRYVPLGMNRDDPDNTEYLNRLNQVLLDRLQAGGEAFLSNA
                     VIKGRFALRSCIVNFRTTLFDVEQIPQIVVALGRQADAELRGRL"
     gene            complement(8873..9970)
                     /locus_tag="VFQ79_25395"
     CDS             complement(8873..9970)
                     /locus_tag="VFQ79_25395"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU0143082.1"
                     /translation="MIQRGIGRWKSVLVLLVFSGSGAGYSGQAIIDSMLAPAAKENRR
                     NTEGDIVVRKDGTFLAAWSDFYGGSRDDSAGRISAATSSDGGKTWSQRFTLQPNIGKQ
                     NVMSASFLRLRSGDILLFFAVKNSPEDLYMMVRRSSDDGKTWSEPVRVVREPGYYGMN
                     NDRAVQLASGRIVCPVYFTEKVWTKTERFRTVSYYSDDDGRTWKRGRTVLECPQRGAM
                     EPGLLELEDGRLLQIIRTQMGMIWHALSSDGGDTWTSPEPWTMAAPEAPSTLVRLPKT
                     GELLLIYNPTVKLGTGHSGPRTPLVAAISRDEGRTWSRPKTIEGDPAATYAYTSVTFH
                     QERALLTYYYAKDGLLSLKFKSIPVDWFRED"
     gene            complement(10003..10203)
                     /locus_tag="VFQ79_25400"
     CDS             complement(10003..10203)
                     /locus_tag="VFQ79_25400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143083.1"
                     /translation="MGLILDSTAAVAAEREGKTARQLLESVALQSGDEEIALSVVTVL
                     ELAHCAPGGTDRPAAVKGRPAV"
     gene            complement(10194..10478)
                     /locus_tag="VFQ79_25405"
     CDS             complement(10194..10478)
                     /locus_tag="VFQ79_25405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143084.1"
                     /translation="MIRCMEPLRISEGELAKDVRSILKRVETGSEVIIERDAQPVAII
                     RPVEPVRRRISECIALMPADSTATIDAGFAEDVEAAIAAHRESLEPPAWD"
     gene            10623..11759
                     /gene="carA"
                     /locus_tag="VFQ79_25410"
     CDS             10623..11759
                     /gene="carA"
                     /locus_tag="VFQ79_25410"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU0143085.1"
                     /translation="MDTATLALEDGMVFEGRSFGAPVEKRGEVVFNTAITGYQEIFTD
                     PSYAGQIVVLTNPQIGNYGANDEDSEASRAFLEGLAVREFSPLASNWRSEEEANYFLK
                     KYEIPIITGIDTRKLVRHLRTRGVMRGVLSAAGGNAAELVEKARSTPSMTGLDLATRV
                     STQKSYEWDKGIKPCSPSDLVRQAPDARFHVAAYDFGIKWNILRCLHQTGCRVTVVPA
                     GTAAEDVLALNPDGVFLSNGPGDPEALAFQSAQVQKVIGKKPIFGICLGQQVLARALG
                     GKTYKLKFGHRGANHPVLNKLTNRVEITSHNHGFAVDPDSLNANEVELTHINLNDQTL
                     EGFRHRNEPVFCVQYHPEAAPGPRDSLYLFDDFVKLMSEFRKAK"
     gene            11765..15010
                     /gene="carB"
                     /locus_tag="VFQ79_25415"
     CDS             11765..15010
                     /gene="carB"
                     /locus_tag="VFQ79_25415"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU0143086.1"
                     /translation="MPRRDDLHRILIIGSGPIIIGQACEFDYSGTQACRALKADGYEV
                     VLVNSNPATIMTDPDLANRTYVEPLTTEFVEEVIRREKPDAILSTVGGQTGLNLSVAL
                     AEAGILDKYGVELIGAKIDSIKKAEDRLLFKDAMRKIGLETPQSQLVNKLADGFDFAE
                     RTGFPLILRPSFTLGGSGGGIAYNGEEFEEILSRGLDLSPVHEVLIEESVLGWKEFEL
                     EVMRDLADNVIIICSIENFDAMGVHTGDSITVAPAQTLTDREYQMMRDASIACLREIG
                     VDTGGSNVQFAIDPKTGRMVIVEMNPRVSRSSALASKATGFPIAKIAAKLAVGYRLDE
                     IRNDITLKTPACFEPTLDYVVVKIPRWQFEKFPNTEPVLGTQMKSVGEVMAIGRTFKQ
                     ALGKGIRSLETGKPTASVEYDPALIPPRLITPNPDRLNYIMFAFERGHTVEQVHEMTA
                     IDPWFLRQVKEVVELREELAQETLETVSRDALLKAKRDGLSDATLGRAWNADRMAVRE
                     RRKELGIRAVFNRVDTCAAEFESFTPYLYSSYESECEADPSDRKKIMILGSGPNRIGQ
                     GLEFDYCCCHASFALQEFGIESIMVNCNPETVSTDYDTSDRLYFEPLTLEEVLNVYDT
                     EKPDGIIVQFGGQTPLNLALPLKAAGLPIIGTQPENIDLAEDRKLFGKLLEELAIPSP
                     ENGTATTVEEACEIARRIGFPVLVRPSYVLGGRAMVVTYDEDTVRRYMREAVVFSHER
                     PVLIDRFLEDATEVDVDAISDSEDVLIAGIMEHIEEAGVHSGDSSCVLPATSIGRAQL
                     DTIIDYTTRLAKALNVIGLMNVQYAIKNGTVYVLEVNPRASRTVPYVSKATGVPLPKV
                     AVGLMLGKKLRDLTALTGGVTSGLLQVSQYFVKSPVFPFNKFPGADPVLGPEMRSTGE
                     VMGVGENFGEAFAKAQLSAGTPLPDQGAVFISVNDRHKTEAARIAKQFREAGLRVVAT
                     RGTAAALRAQGVDCKTILKVNEGRPNAVDLMKAGSIQLVIYTATGAHSFTDEKAIRRT
                     AVAYRIPCITTISGARAAAEALESRRRDPIRVWNLQELHSRGMEPASASHS"
     gene            complement(15123..15410)
                     /locus_tag="VFQ79_25420"
     CDS             complement(15123..15410)
                     /locus_tag="VFQ79_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143087.1"
                     /translation="MSIYEDRRDDLDQYEFMMGTARGRLAVTLDVLTDALVLVGQHGV
                     YCTSTRNPAKPAMDVQLIDRLLNDAKQLVSDVMSELAASRREAQKPDNPPA"
     gene            complement(15421..16797)
                     /locus_tag="VFQ79_25425"
     CDS             complement(15421..16797)
                     /locus_tag="VFQ79_25425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0143088.1"
                     /translation="MSLTRRSFFLIGAAPLIAQQRPAPRPNVLLILADDLAAWMLGCY
                     GNKEIRTPNIDLLARAGTRFANSFVCTPICSASRATLFTGRTPMQHGIHDFLTAKPIE
                     SPPQGQAAPPPSFAKEVMISDILSAQGYNCGYVGKWHMGEDQSPGHGFDYTYTMLGGS
                     RSYQDPAMSLNGKMIEEKGYLTELMTNRAGEFLDKQNPDKPFFLTVAYLNPHTPYTGH
                     PQKYYDMYANSDFKQSGWLPAAPNALREKEMLQDTVGNIRRAAASVTALDDQIPALLA
                     RLKQRGLQDNTLVVFTGDNGYLLGRHGLWSKGLASDPINMYEEVMQVPMIWHWPGRVP
                     VEAARPELVSFYDFLPSICEVTGTAPPSGRNLCGRSYASLAQGRPLPRRQPWNEVVFG
                     YFRNTAMVRDPRFKLVIRNEGKGPNELYALRVDPEEERNVYSNPEYITVRDRLRSQLD
                     AWLKKHSS"
     gene            complement(16811..17623)
                     /locus_tag="VFQ79_25430"
     CDS             complement(16811..17623)
                     /locus_tag="VFQ79_25430"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0143089.1"
                     /translation="MSTAIPGKHFLSNLIERRTLLFQLVRRDFKQRFVGSAAGWLWGL
                     IHPLVLLLSWTFVFKICLRAPIPPGEATDNYTMFLFCGFLPWMLFQETVQRSSTSLLE
                     HSNLITKTVFPAEIVPVSIFLSALLSHLMALGLLIAAAIVWLGYVSPMFVLLPVYMAL
                     IGLFAVGVGWIVSSLQVYIRDTAQVVMVVLTLWFWMTPIFITIEQVPPQFRFLMRVNP
                     MAYMVRAYRDRLLSYRLPSIDELAIMGVCAAAAFVIGGLFFRHLKRGFADVL"
     gene            complement(17662..18003)
                     /locus_tag="VFQ79_25435"
     CDS             complement(17662..18003)
                     /locus_tag="VFQ79_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143090.1"
                     /translation="MPIRPEYRKYYDARWRRLRLTLLEAAGNVCQNCGSPHRLLNVAH
                     LSHDPADRTSLVVLCPRCHSLHDTPQRLAVTRRTRARKRGQLWLSQELEIAPLPVRMW
                     PAKLRQLRLFG"
     gene            complement(18026..19126)
                     /locus_tag="VFQ79_25440"
     CDS             complement(18026..19126)
                     /locus_tag="VFQ79_25440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0143091.1"
                     /translation="MKFCFLTSTPQNVTAGSGTFRGIHVLASALRGLGHPVEVVTPEK
                     HSRIFTLERLRFNESLRRKDLSSFDLVVGFDLDGYRIAGRGPIPHIAAIKGVIADELR
                     FERGITRTMLSIQAACERIHVQRAAGVITTSKYAAEQLKRLYRFPQPVHVVPEMLDLA
                     QWRELLDGSGVAPDPNRFSILTVCRFYPRKRLDLLLRAAALLRERIGNLQFRIVGGGP
                     ESKRLRELSRALRLEGTVEWLGDVTQARLAAEYTSCDVFCLPSLQEGFGIVLLEAMAA
                     GKPIIAARAAAIPEVVPEACLVEPGDARALASAIGQLYRSPDMRRVLAERGLARVQCF
                     DAPKVAVQFVDAVNDIMSSARQAVFTSAGILL"
     gene            19348..20550
                     /locus_tag="VFQ79_25445"
     CDS             19348..20550
                     /locus_tag="VFQ79_25445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0143092.1"
                     /translation="MASDSTALEIAASRKAGPAARAGVTALVLLSFGHFFIDLYSSAL
                     GALQPFLVDKLGLTLTQAGLLGGAMVFTSSVMQPVYGFLSDRFHTRLFAALAPAVAGI
                     GISALGLAPGFGWLLLMIVIGGAGIASFHPQASALATTGVASGRGRSMAIFISAGTLG
                     MALGPTYFSWVAITLGLANTYWAAIPGILMTILLVSMLPPLQHGAASRQGGFDLAPFK
                     AVWKPLTILYFLVFIRSIVQITFAQLLPLYLHRERGFSIAGASYALSLYLTAGAIGGF
                     LGGHLSDRWGGRKVILISMISCVPFLALFFLTHGWISIAGLAMGGLTLLFTIPVNVVL
                     AQQLAPTQTGTVSALMMGFAWGVAGIIFIPLTGLASDHFGMHPALMSLTVFPLIGFFL
                     TLKLPKGM"
     gene            20552..21247
                     /gene="queC"
                     /locus_tag="VFQ79_25450"
     CDS             20552..21247
                     /gene="queC"
                     /locus_tag="VFQ79_25450"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEU0143093.1"
                     /translation="MDQAPKAVCLLSGGLDSSTCLAVARADRFETYALSFDYGQRHRV
                     ELDAARRVAASLSAREHLIVPVGLGLFGGSALTGDMAVPKSRPAAEIASGIPITYVPA
                     RNTIFLSLALAWAEVLGSTDIFIGVNAIDYSGYPDCRPEFLDAFERVANLGTKAGVEG
                     SGKIRIHAPLKELSKAAIIRLAIGLGVDLSLTHSCYDPDREGRPCGACDSCLLRRKGF
                     AEAGTADPLEYRE"
     gene            21244..21927
                     /locus_tag="VFQ79_25455"
     CDS             21244..21927
                     /locus_tag="VFQ79_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEU0143094.1"
                     /translation="MKISEIFYSIQGEGMLVGTPSVFVRTSGCNLRCHWCDTPYASWK
                     PEGEDLYLGAILAEVRRYWADYVVITGGEPMIVKEMVRFTQQLKTLGFHITVETAGTV
                     FEPVDCDLMSISPKLSNSTPTTIEGGRWAAQHERLRIQPEVLKKLIGAYPYQLKFVVE
                     KPDDIAEIRSLVNDLDADRRRVVLMAEGTDPETLHERSLWLADICREEKYRFTPRLHI
                     DLYGNRRGV"
     gene            complement(21936..22562)
                     /locus_tag="VFQ79_25460"
     CDS             complement(21936..22562)
                     /locus_tag="VFQ79_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEU0143095.1"
                     /translation="MILYLHGFASGPASRKARYFQQRLAARGIDLLVPAMDEGDFRGL
                     TITGQLQLLERLAGREPVTLIGSSMGGYIAALYASRHPETEKLVLLAPAFGFTHRWPE
                     SLGEATLRQWQTSGALLVHHYATGEMREVGYQLIEDGKRYEDYPDFHQPTLILHGSQD
                     DVVPLAYSEQFAATHPNVELKVLDSNHELTNVLGEAWREAERFLFARR"
     gene            complement(22559..23446)
                     /locus_tag="VFQ79_25465"
     CDS             complement(22559..23446)
                     /locus_tag="VFQ79_25465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143096.1"
                     /translation="MVLLPAWLLAATLAFYFGAGGSAAAPETPGAAVQAALNRLSEEA
                     EIFIKNAPSLVARETLRQRAVKTWLPSSRSRPRPPVYQQRVVVSEYGYASLAESPGVL
                     HELRKVISVDGRRILRAREARTALTMGVTTPDDRLKQKLLRELESYGLSGAVTDFGPM
                     ISLFSRAALPNYSFRLVRREFIGAEQAIVLSYRQKDGGGLTIFENREALKLILQGEIW
                     LREPDFVPLKITTLNERKHGGEKLRDEGAVEYTRTSHGVLVPVSVVHRQFRGSTLVAE
                     NLFLYSEFGRLSAEPPFEK"
     gene            23536..23775
                     /gene="acpP"
                     /locus_tag="VFQ79_25470"
     CDS             23536..23775
                     /gene="acpP"
                     /locus_tag="VFQ79_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0143097.1"
                     /translation="MSVEQKVKQIIVEQLGVDEGQVDSTASFVDDLGADSLDIVELVM
                     AFEEAFDLDIPDEDAEKIATVKDAIDYIEGKAAKK"
     gene            23825..25066
                     /gene="fabF"
                     /locus_tag="VFQ79_25475"
     CDS             23825..25066
                     /gene="fabF"
                     /locus_tag="VFQ79_25475"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU0143098.1"
                     /translation="MSRRVAVTGVGLLTPLGLGTEETWEAVRSGKSGITRISAFDPSA
                     YNCQIAGEVKDFDPTRYIDRKEIKKMGRFIQFAIAATEFALSASGLKIDESSAERSGV
                     YIGSGIGGFEVIEREHKILLERGPGRISPFFIPATIINLAAGYVSIRTGAKGPNSATA
                     TACTSSAHAVGDSFKIIQRGDADVMICGGAEAPITEMGIGGFAAMRALSQRNDEPGRA
                     SRPWDRDRDGFVVGEGAGILILEELEFARKRGANILAEIVGYGMTADAFHMTAPSEDG
                     DGAFRVMRLALKDAGLQPEQIGYINAHGTSTPLGDKLETLAIKRAFGEYARKLAVSST
                     KSMMGRLLGGAGGVEAGITILAMRDGMLPPTINYECPDPECDLDYIPNEARPAQVDYA
                     LSNSFGFGGTNGCIIFKRYRG"
     gene            25073..25843
                     /locus_tag="VFQ79_25480"
     CDS             25073..25843
                     /locus_tag="VFQ79_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEU0143099.1"
                     /translation="MKIIVAIKQVPARDSVLQVDSSGKWIRESDLSFEVNEPDAYALE
                     EALQLKERYGGEVIALCAGPARVSQAVREALAKGADRAIQIESDDLGAADSLGIARLL
                     ASAIKAEQPDLVLTGLQSDDLGYGQTGVIMAELLGFPHSTIIMQVEAAGSSVRVKREL
                     ENGWFQWMEIPLPAVLTIQSGISKLRYATLMGIKKARTKEVKRLTAAELGGLAAPVLA
                     LEKVCLPQKTKETQMLDGTPAEAAAALVEKLRFEARVL"
     gene            25840..26814
                     /locus_tag="VFQ79_25485"
     CDS             25840..26814
                     /locus_tag="VFQ79_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU0143100.1"
                     /translation="MNVLVVLEQHGGEWNRMSFEALSAGQQIAADMGGRASAAVLGNG
                     LSKLADELRGTDLETVYAVEHPLLENYTTDGYTATLEALLKTRKPSLVLFPHTYQVRD
                     FAPKLATRFNQVLVSDMIGHRVESGSLILVRQYFQGKVNGDLRALGDGPHFGSIQAGA
                     YRSDRVAGGSATVEAFTPDLNATSIRNKPEPPFREAERAVDLTTADIIVSVGRGIKEK
                     DNLPLVEELAKTLNAELAASRPICDNGWLPMERQVGSSGQTVSPKLYIAVGISGAIQH
                     LVGMKGARTIVAINKDENAPIFEVADYGIQGDLFEVVPALVKELKKIR"
     gene            26935..>28077
                     /locus_tag="VFQ79_25490"
     CDS             26935..>28077
                     /locus_tag="VFQ79_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0143101.1"
                     /translation="MSRLNAFTLLLAGLSAFAITSTANAQVTGTISGFVTDPTGAAIP
                     GARITATLIEQNVSRTAESNDLGYYVFNALPPGLYALAGEKEGFQRLVRTEISLAVNQ
                     NLRVDLGLKLGQLAESVEVTATAPLVDTRSPALSGLVDDRRVVDLPLNGRNVISLAAT
                     LPGILSVVAPQQLSDSRSGPLMNVNGSVTTQNMFTFNGGIFVHPSRNTGLNYPPPDAL
                     REFSIQTQSFSAEYGRNAGSQVNVVSKSGTNEFHGSAWEFLRNNHLNARNFFASRVPT
                     KIQNQFGLAAGGPIRKDSIFIFGSYQGLRDRAEAVGVSADVPSPAQRTGDFTGLGRTL
                     TNPVDTLTGQPFTDPSGRPCVSGNVISPGCISPVAQNLLPLIPLSPT"
BASE COUNT         5863 a         8292 c         8454 g         5468 t
ORIGIN      
        1 cgatgggacc cggaaaaaga acattttctt tccgacgccg aagccaatct gctggccgac
       61 cgcccccgcc gtaaaggttt cgaattaccg aagctgtagt gctgcttatc gagctccgga
      121 gcgcagatgc ggagatgtag tatgctgtct ccacccacat gagattcctg gcattacttg
      181 ttttgcccgc gttgctgggc gcgcaggttc cttcgaagga agacgtgctg cgcgcgatgc
      241 gcaaagcagc tgatttctac cggcacaagg tgtctaccca tggcgggtat catttttact
      301 acactgctga tctgagttac ggcaggtccg aatccgccga aggacccacg caggtggaag
      361 tacagcgcga agcaacccca atcgtggcgc tggcgtacct ggatgcctat gaggtgacgg
      421 gcgaccgcac ttatctcgac tacgcgcgcg acgcggcaca cgctctggtg aaaggccagc
      481 tttgcagcgg cggctgggat tacctgatcg aattcgatcc ggaaaagcgc aaacgctatc
      541 agtaccggga agacggcgat tgcggcggag cgggacgcaa cgtcactact ctcgatgaca
      601 acgtcacgca ggcggccatc cgggttttga tgcgcgtaga caaggcactc gatttttccg
      661 acgccgccat tcacgaatcg gcccggcttg cgctcgaccg tctcatagcg gcgcaatacc
      721 cagtgggagc gtggccgcag cggttcagtg cgccgcccga tttttccagg tttccggtga
      781 aacgcgccag ctatccggag acgtggtcga gaaaatggcc cggaccggaa tttcagcagc
      841 attacacgtt caacgacaac tccatcgccg acgcgatcga caccatgctg gaggcggcgc
      901 gaatctacaa cgagccgcgc taccgcgcgt cggcggaaaa gggcggccag ttcattctgc
      961 tggcgcaaat gcccgatcct caaccggcgt gggcgcagca atacgatgcg gacatgcacc
     1021 ccgcgtgggc gcgtgtcttc gagccccctt ccgtcaccgg cggagaatcg caggggattc
     1081 tgcgcgtact gatgacgctc taccgcgaaa cgggcgatcg gaaatacctg gagcccatac
     1141 cgcgggcgct tgaatatctg aagcgctcgt tcgttccgcg agcggatgcg gaagccttcc
     1201 ggcgtcttcc atccggtccg gtgctcgcca ggttctatga gctcaaaacg aaccggccgt
     1261 tgtatatcac caagggaaca cgcctgaatg tcgccggtat gggcagccgc ctcgtggatg
     1321 gatacgaaat cagctacact cccgaatcgg tgatcacgca ttacggcgtt ctggtaagcg
     1381 gaaaccaact taacgtcatc gaagaagact accggaaact gggggcagcg gctccgaagg
     1441 gtgtccgcag gccggacctg ttgcgcggac tgtcgccctg gagcgaacgc ccgtttcgga
     1501 tgaccgaagc cccgccgcct ccgaacgaag tgaaagaact gatcgcttcc atggatgggc
     1561 gcggcgcctg gacaaagatg gggaccatcg gccgtgccga ccggcttgtc ttcgccttcg
     1621 ccgccaaaga cctggttctc cgcatcggac gcgggcgctc ggatggatcc gcaggcgatg
     1681 gtttgaattc ccaggccgac atcattccga ttcaggaaaa catgacggtg gaacttttcg
     1741 ccggaccgca gcctccgccc gtcagcatca tcagttcggc ggatttcgcc ggcaacctgg
     1801 cgcggctcgg ccgctatctc accgcgtcgc gacaaacccg cgcggggcgt taaggaataa
     1861 cataagaggt catgcggcgg cgggatttct tcaagtcagc ggcggtattg gccgcacctc
     1921 gcgcggaaca attccaattg gctacgttcc aatcggacat taccccttcg atcggcgggc
     1981 ctttatttaa taatgtgacg gcgcggaaga ttgtcgatcc gctgcaggcg cacggcgtag
     2041 tcctgcttgg cggcggccgg cccattgtcg tcgcggtgct cgactggtgc gaaatccgca
     2101 atgacgctta caacctgtgg cgcgacgtga tggcggaagc cgcacgcaca tcgcgcgaac
     2161 gcgtgctgtt gacttgcgtc caccagcatg acgcgcccta cgtggatacg gaagcgcaac
     2221 ggctgttcga agccgccaaa gcaccgggaa gaatctgcga tccgggcttc aacgaacatt
     2281 gcgcgcgcaa agttggcgcc gcgcttcagg caagcctcaa ctccgcgcgc cgcatcacgc
     2341 atattggtac gggacaagcc aaggtggaac gtgttgcttc caaccgccgc tacgtcaggc
     2401 cggatggcgg ggtgagtttc gggcgtacga gcgcgacaaa ggacccagcc ctgcgcgcat
     2461 tgcccgaagg caccatcgac ccttggctca agaccatcag cttttgggag gaggaccggc
     2521 cggttgcagc gctcagttgc tattccacgc atccgatgag ctattacggg caaggcgacg
     2581 tctccgcgga tttcgtcgga atggcccgcg cgcgccggca gaaggacacg cccggcaccg
     2641 cgcagatcta tctcaccggg tgcagcggcg acaccatcgc gggtaaatat aacgatggca
     2701 gcccggcgaa tcgtcccgtc ctggccgccc ggatgtatca gggcatggtg ggcgcctgga
     2761 aagcgaccaa acgccggccg ctcaaaaacg tgcgattccg gtccgttccc atgcacctgg
     2821 cgccgcgagc aaccccgggc ttctcgcccg cggagatgcg caaaaccctt gccgatgaat
     2881 ccaagccata tctggcgcgg gccgaagcgg cgttgggact cagttggaga aagcgtgtgg
     2941 atgcgggcga ggcggtcgat gtacctgctg tcgatttcgg cggagcccac ctcgtgctgc
     3001 tgccggccga ggcgttcgtg caatatcagt tgtgggcgca ggagctgaga ccggattcgt
     3061 ttgtcatgac ggcagggttc agcgaatcgg cccccggcta catccctacg gcgcaggccg
     3121 cggccgaagg gtatgacgat cactatagct gggtcgcatt ccccgagtgt gaaaacacca
     3181 tgcggaacgc cttgcgcgat gccctgcgga ggtatgacta gttgaaccgg cggcgattcg
     3241 tcacaacact cggcggcatt ccagccgcgg ccggtgcttt cgaagaacgc cggcccaaca
     3301 tcctgctcat cttctccgac gaccacgcct accaggccat ctccgcctac gatggccgcc
     3361 tgaaccatac gcctaatatc gaccgcctgg cgcgcgaggg cgttcgattc gacaacgcgc
     3421 tggtgaccaa ctccatctgc gcgccttcac gagcagtggt gctgacgggc aagtactcgc
     3481 acctgaatgg tgttctcgat aaccgtcaga ccttcgacgg tgctcaacag acctttccca
     3541 aaatcctgcg gcaagccggt tatcagaccg cactctttgg gaagtggcac ttgaaatcga
     3601 acccgacggg attcgatgtg tgggaagtat tgcccggcca gggcagctac tataatccgg
     3661 atttccttac gcctgacggc aggaagcgcc gcgaaggcta tgtcaccgag ttaatcaccg
     3721 gccttacgct ggactggctg gaaaacgggc gcgacaagac caagcctttt atgctgatgt
     3781 gccagcacaa ggcaccgcac cggaactgga tgcccgcgcc gtcgaagctg tacctgtacg
     3841 atggcgtcac ctttccggag cctccgaatc tctttgataa ctacgagcac cgcgccacac
     3901 ccgcgcgcaa acaagccatg gagatcgacc gccacatgac gctgatggcc gatctcaaaa
     3961 ttctgcccgc cggcggcacc ccgccgcagg acgccgacta ccggcgctac ctgggcgagt
     4021 acgggcgcat gaacgaggcg cagaagcgaa tgtgggacgc cgcttaccgc cctcgcaacg
     4081 aagccttcgc ccgtgccaat ccgcaaggca gggacctggt ccgctacaaa taccagcgct
     4141 acctgcaaga ttacttgcgc tgcatcgcgt ccgtggacga cagcgtcgga agaattctga
     4201 attggctgga cgccacgggc gaggctgaga acacgctggt ggtctacgct tcagaccagg
     4261 gtttctatct gggggaacat ggctggttcg ataagcgctg gatctacgaa gagtccattc
     4321 gcacgcccat gctggcccga ttccccggca aggcacgcgc tggatatgta tgcgaaagca
     4381 tggtggcgaa tctcgatctg cccgaaacgt tcctggatgc cgccggactc cgcgtgccgg
     4441 cggatatgca gggccacagc ttcctgcggc tcctgcgcgg tgaaaccccc tccggctggc
     4501 gccagtcgtt ctactatcac tactacgaac ggggtgagca cgacgtcgct ccacattacg
     4561 gagttcgcac ggatcgctat acgctcgcgc actattacga taccggggag tgggagctct
     4621 tcgaccgcat gaaagacccc caccagatgc gcagcgttta cgcggaggca tcgtacgcgg
     4681 acgtcgtgaa aaaactcaag gcggaactga cgaggcttca gcgcgagctt cgcgtgcccg
     4741 atagcaagcc gatttgacgc ttccgaaggc aatccgttaa tctctagttg gattgaggtg
     4801 attgccgatt gccgatgaat atttccgtaa aaggcgaata tgcactgctc gcgctatttg
     4861 accttgccag ccagcggccc ggtgaacccg tgaagatcgc ggatatcgca cggcgccaga
     4921 agattcccca gaagtttctc gagttgattt tggctaccct caagcaaggc gggtttgtgg
     4981 aatcgcgccg cggcgcggag ggcggctatc tgctggcgcg tccggcggat tcgatcacca
     5041 tcggcgagct tttgcgattc atcgaggccc cgcgttcaaa ccgttcgaaa ggaaaacgca
     5101 aatcggattc gccattcgcc agtatgtggc gccaggtgga tgaggcgatt tcgagtgtgg
     5161 tggaccagac gacctttggc gaactgactc gcgcctggaa agaaaaccaa atgaactatg
     5221 taccgaattg ggagatctga aagtgcagcc gacaacaggt aagtcctttt ttgaagataa
     5281 ttcgttcagc atcgggcgga cgccgctggt aaagctcaat cgcatcacgg acggtgcgcc
     5341 ggttacgatg ctcgccaaga tcgaaggccg gaatccagcc tactcggtaa aatgccggat
     5401 cggcgcggcg atgatttggg acgcggagaa gcgcggcatt ctaacgcccg gcaaggaact
     5461 gattgagccg acgagcggta acaccggaat tgcactggcg ttcgtcgcgg ccgcgcgagg
     5521 ttatcccatc acgctcacca tgccggagac gatgtcgatc gaacggcgca aagtcttaaa
     5581 agccttcggc gcgaacctgg ttctgacgga aggctcgacg ggcatgaagg gcgcgattgc
     5641 gaaggccgaa gaatttgtgg tttccaatcc gaaccgctac gtcctcctgc agcagttcaa
     5701 gaatccggcg aatcccgata tccatttcca gacgaccggt cccgagatct gggaggatac
     5761 cggcgggcaa atcgacgtgc tggtgtccgg cgtcggcacc ggtggcacca tcaccggtgt
     5821 ttcccggtat atcaagcaca agttgggcaa gccacttgtg tcagtggccg ttgaaccgtc
     5881 caacagcccg gtgattacac aggcaaagaa aggcgagccg cttaagccgg gaccccacaa
     5941 aatccaggga ctcggagccg gattcatacc ggatacgctc gatctgtccg tcgtggatct
     6001 tgtcgagcag gtgacaaacg aggagtcgat cgaaatggca cgacggctcg cgcgcgagga
     6061 aggcatcctt tccggaatct cctgcggagc ggccgtagtc gcggccgttc gcctggcacg
     6121 gcagcccgag tttcacaaca aaaccatggt cgtgattctg ccggactccg gagagagata
     6181 cctgtcgtct gtgctgttcg atggattggt agattgaatg cagtaaagcg ggcgcaagtt
     6241 tgggtgaaag gggtgagtgc gcccgctgtc gtaacgggga tttgatatat aaagcgtaaa
     6301 cgggcgttgt aaagttacac aaaaaaacca aaaatatttc tcttgaccga gcggcggaag
     6361 gtggcgtaca ctggtggcga acaaaaggcg aatggaggac gccgtgtatg ccgcagtccc
     6421 caaatatcta tatcggaccc gccggttggt cgtatcccca ctggaatgga gccgtctatc
     6481 ctgaggcgcg ccccagggga tttcatgcgc tccattttct gtccgaattc acggacttag
     6541 tggaaatcaa tacttcgttc taccagccgc tgcggcctga aatcaccagc gtctggttaa
     6601 agaaagttga ccacaatccc aagtttctgt ttaccgccat gctgaatcgg cggttcacgc
     6661 atgaacgaag ccttgaacct gcggaggtgg tggcgaacaa gcgaggactg tggcctctgc
     6721 tcaaagaagg aaaactcgga tgcctgctga tgcagttccc ctgggcattc cgttttacgg
     6781 aggagaatcg cgccttcttg atcaaattgc gccggacctt ccatgaattc ccgctcgcgg
     6841 ctgagatgag acacagcagt tggatggccg atgaagcgct gggtgtgttc gtggactatc
     6901 gcatcggatt ctgcaacatc gacattccac agcatgtcaa gaccatgccg ccaacggcgt
     6961 tcctgacctc cggaatcgcg tacgtacgtc tgcacgggcg aaacgccggg ttgggcattc
     7021 agaacttcga cgctgcacca caaaacagtc aggattgcga ctatctgtat tcggcggaag
     7081 aactgtccga atggaaaccg cgcatcgagc ggatcgcccg gctggcggaa cggacgttcg
     7141 tggtaacgaa caaccacggc ggcggcagag cgatagtaaa cgcgctgatg ctcgaggaac
     7201 tgttcggaaa aactccggca gccgcaccgg cgggcttgcg ccgcctctat acctcacagt
     7261 tgaaggcact cccgctggaa actccgctac agaacacact attcgaggcc gcctgagaag
     7321 acgggcggat atactctccg ataggagcaa cagtgtatcc ggacgaagat ggagatttga
     7381 gaaagcgggc ggcgccgctc gatatgagcc cggaggaatt ccggcgtctg ggttacacct
     7441 ttatcgataa agtagcagac ctgctggcgg agatccgaag ccgccctgta acaccgggcg
     7501 aaagccccac cagcgtgcgg aatgcccttg gcaacgcgcc gttgccgcag gaggggtttg
     7561 ccgcggagga tgcgttggca agggcctggg aattgctctc cacccactcg ctgctaaatg
     7621 gccatccgcg cttttcgggg tatgtaacgt ctccggcggc gcctgtgggt atgctgggcg
     7681 acctcatggc cgcggcggtt aatccgaacg ccggcgcctg gattctttcg ccgatggcga
     7741 ccgaaataga atcgcaaaca gtacgctgga tcgccgaatt tctgggatat ccggcggatt
     7801 gtggcgggct cctggtaagc ggcggcaata tggccaattt cattggattc ctggcagccc
     7861 gccgcgccaa atcggctcgg gatcttcggg ccgaaggcgt cgaacccgga aaactcgcgg
     7921 cctacgtctc cacggagacc catacgtgga ttcacaaagc cgccgacctg ttcgggctgg
     7981 gaactaacag cattcgatgg attccgatcg atgacgagtt ccggatgcgg accggcgatc
     8041 tcgttgacag catcgagaac gacagggctg ccggattcac accgtttttt ctctccggct
     8101 cggcaggcac cgtcagtaca ggcgcggtgg acccactcga agagttggcg gagatcgcgc
     8161 ggcggtacga tctgtggttc cacgtcgatg gagcatacgg tgcgcccgcg gcggcgctgc
     8221 ctggcgccag tgcgcatttg aaagcattga gccaggcgga ttctctggcg gtggacccgc
     8281 acaagtggct gtatgcgcct ctggaagccg ggtgcgctct cgtacgcgac cgccggcgcc
     8341 tgttcgatac cttcgattat tcgcccccat actatcgctt cgatgccaat gagggcgaaa
     8401 agcctctcaa ctactattca tatggtccgc agaactcacg cggattccgc gcgctgaaag
     8461 tctggctggg cctgatgacg gccgggcgtg aagggttgat ccagatgatc ggtgacgata
     8521 tggaactggc ccgcacgtta ttccgggaaa tccggaatca cccggaattg gaagccttca
     8581 cacaaagtct cagcatcact accttccgct atgttccctt gggtatgaac cgggacgatc
     8641 ccgacaatac cgaatacttg aaccgcctga atcaggtgtt gttggatcgc ctgcaggccg
     8701 gcggagaggc gtttctgtcc aacgcggtca tcaaggggcg gtttgcgttg cggtcctgca
     8761 tagtgaactt ccgaaccacc ctgttcgacg tagaacagat cccccaaatt gtcgtggcgt
     8821 tgggcaggca ggccgatgcg gagttgcggg gccggttata gccgccactc cttcagtctt
     8881 cgcggaacca atcgacggga atgctcttaa acttcagcga gagcaggccg tcttttgcgt
     8941 agtaatacgt cagcagtgcg cgctcctggt gaaacgtcac gctcgtgtag gcataggtgg
     9001 cggccgggtc gccttcgatc gttttcggcc gcgaccaggt gcggccctca tctcgcgaga
     9061 tcgcagccac caggggcgtt cggggtccgg agtggcccgt cccgagtttt accgtagggt
     9121 tatagatcag cagcaactcg cccgttttgg gcaggcggac caatgtgctg ggcgcttccg
     9181 gcgccgccat tgtccacggt tccggcgagg tccacgtatc gcctccgtcg ctcgacaagg
     9241 cgtgccagat catgcccatt tgcgtacgaa tgatctgcaa cagccgtccg tcctccaatt
     9301 cgaggagccc gggttccatg gcgccgcgct gcgggcattc caaaaccgtg cgcccgcgtt
     9361 tccacgtccg cccatcgtcg tccgagtagt aggagaccgt gcggaaccgt tccgtttttg
     9421 tccacacttt ttctgtgaag tagacagggc agacaatacg tccggacgcc aactgcaccg
     9481 cccggtcgtt gttcattccg tagtagccgg gttcgcgaac gacacggacc ggttcgctcc
     9541 acgtctttcc gtcatcggac gatcgccgga ccatcatata caggtcttcg ggcgaattct
     9601 tgacggcgaa aaagagcagg atatctcccg atcgcaagcg cagaaagctg gccgacatca
     9661 cgttctgctt gccgatattc ggctgaaggg tgaatctttg agaccacgtc tttcccccgt
     9721 cggacgacgt ggcggcggag atacgtccgg cggaatcatc gcggctgccg ccatagaagt
     9781 cggaccacgc cgccaggaac gtaccatcct tccgcacgac aatatcgccc tccgtatttc
     9841 gacgattctc ctttgccgcc ggggcaagca tggaatcgat gattgcctgt ccggaatagc
     9901 ccgcgccgga gccggagaaa accagaagga caaggaccga tttccatcgc ccgattccgc
     9961 gttggatcat actagaggcg taccagcgcg ggggcgggcc tgtcaaaccg ccgggcggcc
    10021 cttgactgct gccggtcgat ctgtcccgcc cggagcgcaa tgcgcgagtt cgaggacggt
    10081 cacaacagaa agcgcgatct cctcatcgcc actctggagc gcgacggatt cgagtagttg
    10141 ccgggcagtc tttccctcac gttcggcggc aacagccgcg gtcgagtcga gaatcagtcc
    10201 caagccggtg gctccagcga ctcccgatga gcggctatgg ccgcttcgac gtcctcagca
    10261 aagccggcat cgatagtagc cgtggagtcg gcgggcatca gagcgatgca ctcagagatc
    10321 ctgcggcgca cgggttcaac tggcctgatt atggccaccg gctgcgcgtc acgctcgatg
    10381 atgacctcgg atccagtctc gacccgcttg aggatggacc gcacatcctt tgcgagttca
    10441 ccttcactga tacgcagcgg ctccatacat cggatcatag cgtttcctcg tcctccttgc
    10501 ctcgtcggat tcggcggcct gacagagaat cgggtcaaac tgcccgcccg ccgcgacccg
    10561 cttgatggag cgggctgagg catgttccaa taaaagttat accttcctgg aggaccagcg
    10621 gattggacac agctacgctc gccctggaag acggcatggt cttcgagggc aggagctttg
    10681 gtgcgcccgt agaaaagcgc ggtgaagtcg tttttaacac tgccatcacg ggatatcagg
    10741 agatcttcac cgatccttct tatgcgggtc agatcgtggt tcttacgaac ccgcaaattg
    10801 gcaattacgg cgccaacgac gaggatagcg aggcatcgcg ggctttcctc gaagggctcg
    10861 cggttcggga attctcacct ttagccagca actggcgatc cgaggaagaa gcaaactatt
    10921 tcctcaagaa gtatgagatt ccaatcatca ccggaatcga tacccgcaag ctggtgcggc
    10981 atctgcggac tcgcggcgtg atgcgcggtg tgctatccgc ggccggcggc aatgcagcgg
    11041 agttggtgga aaaggcccgg agtacgccat cgatgaccgg actcgacctg gctacccggg
    11101 tgtcgacgca gaaatcttac gagtgggaca aagggattaa accctgctcg ccttcggatc
    11161 tggtccggca ggcgccggat gcccgttttc atgtggcagc gtacgacttc ggcatcaagt
    11221 ggaatatctt gagatgcctt caccagacag gttgccgggt gacggtagtg cccgcgggca
    11281 ccgcggcgga ggacgtcctg gctctcaacc cggacggcgt gtttctttcc aacgggccgg
    11341 gcgatccgga agcgctggcg tttcagtcgg cgcaggtgca gaaggtcatc gggaagaaac
    11401 ccatcttcgg tatttgcctg ggacagcagg tgctggcacg ggcgctcggg ggcaaaacgt
    11461 acaagctgaa gttcggacac cgcggagcga atcatcccgt gctgaacaag ctgacgaatc
    11521 gcgtagagat cacatcccac aaccatggtt tcgcggtgga cccggattcc ttgaatgcca
    11581 atgaggtgga actgacacac atcaacctga acgatcagac cctcgagggg ttccgccacc
    11641 ggaacgaacc cgtcttctgc gtgcagtacc accccgaagc ggcgccagga ccacgcgatt
    11701 cgctatatct gttcgatgat ttcgtgaaac tcatgagcga gttccggaaa gctaaataac
    11761 gaacatgccc agacgtgacg atctccaccg gattctgatt atcggctcgg gtcccatcat
    11821 tatcggccag gcctgcgagt tcgattattc cggaacacag gcttgccgcg cactcaaggc
    11881 ggatggatac gaagtggtcc tggtgaattc caacccggcc accattatga cggaccccga
    11941 ccttgcgaac agaacttacg tggagccgtt gacaacggag tttgtggaag aagtgatccg
    12001 ccgcgaaaaa cccgatgcga ttctgtccac ggtcggtggg cagacggggt tgaacctgtc
    12061 ggtggcgctg gccgaagcgg gcatcctgga caaatacggc gtcgagttga tcggcgcgaa
    12121 gatcgactcc atcaagaagg ccgaagaccg gctgttattc aaagatgcga tgcgcaagat
    12181 cggactcgag acgccgcaat cgcaactggt caacaagctc gccgacggtt tcgactttgc
    12241 cgaacgcacc ggttttccgc tgattctgcg gcccagcttc acgctcggcg gcagcggcgg
    12301 cggcatcgct tataacggcg aagagttcga ggagatcctg tcgcggggtc tcgatctgtc
    12361 ccccgtgcac gaagttctga tcgaggaaag cgtcctcggc tggaaggagt tcgaactgga
    12421 ggtgatgcgc gacctcgcgg acaacgtcat catcatctgc tcgatcgaaa acttcgacgc
    12481 gatgggagtt cacactggcg attccatcac ggtcgctccc gcgcagacgc tgacggaccg
    12541 cgagtatcag atgatgagag acgcctccat cgcatgcctg cgcgagatcg gtgtggatac
    12601 cggcggctcg aacgtgcagt tcgccatcga tccgaaaacg ggccgcatgg tgatcgtgga
    12661 gatgaacccc cgcgtttcgc gaagctccgc gctggcttcg aaagcaaccg ggttccccat
    12721 tgcgaagatc gcggcgaaac tggctgtagg ataccggttg gacgagatac gcaacgatat
    12781 tacgttgaaa acgccggcgt gtttcgaacc gacgctcgac tacgtggtgg tgaaaatccc
    12841 gcgctggcaa ttcgaaaagt tccccaacac ggaaccggtg ctgggaacac agatgaagtc
    12901 tgtcggagaa gtgatggcca ttggccggac cttcaaacag gcgctcggga aaggcatccg
    12961 cagcctggag acgggcaaac cgacggcgtc ggttgaatac gatccggcgc tgattccacc
    13021 ccggctgatc acacccaatc cggaccggct gaactatatt atgttcgcct tcgagcgtgg
    13081 acacacggtc gagcaggtgc acgagatgac ggcgatcgat ccctggttcc tgcgccaggt
    13141 gaaagaggtc gtcgagctcc gcgaggaact ggcgcaggaa acattggaga ccgtttcacg
    13201 cgatgcgctg ttgaaagcca agcgtgatgg cctctctgac gcgacactgg ggcgagcctg
    13261 gaacgccgat cgcatggccg ttcgcgagcg ccgcaaggaa ctcggcatac gagcggtgtt
    13321 caatcgcgtg gacacgtgcg ccgccgagtt cgaaagcttc actccgtacc tgtactcgag
    13381 ctacgaatcg gaatgcgagg cggacccgtc cgatcgcaaa aagatcatga ttctcggatc
    13441 gggcccgaac cggattggcc agggtttgga attcgattac tgctgctgcc acgcttcttt
    13501 cgcgctgcag gagttcggaa tcgagtcgat catggtcaac tgcaatccgg agacggtatc
    13561 gacggactac gacaccagcg accggctgta cttcgaaccc ctgacgctcg aagaggtcct
    13621 gaacgtctat gacaccgaaa agccggatgg catcattgtg cagttcggcg ggcagacgcc
    13681 gctgaacctt gcgctgcctt taaaagcggc aggactcccg atcatcggca cacagcctga
    13741 aaacatcgac ctggccgaag atcgcaagtt gttcggaaag ctgctggagg aactggcgat
    13801 tccgtcacct gaaaacggca cggcgacgac tgtcgaagag gcatgcgaaa tcgcgcggcg
    13861 aatcggcttc ccggtgctgg tgcgtcccag ctacgtctta ggagggcgcg ccatggtggt
    13921 cacctacgat gaggataccg tccgccgcta catgcgggaa gcggtagtgt tttcccatga
    13981 gcgaccggtt ctgatcgacc ggttcctgga agacgcaacc gaagtggacg tggatgccat
    14041 ttccgattca gaagatgtcc tgattgcggg tatcatggaa cacatcgagg aagcaggcgt
    14101 gcactcgggc gacagctctt gcgtgcttcc cgccacctcc atcggcaggg ctcagctaga
    14161 caccatcatc gattacacaa cacggcttgc caaggcgctg aatgtcattg gtttgatgaa
    14221 cgtgcagtat gcgatcaaga acgggaccgt gtacgttctg gaagtaaatc cccgcgcctc
    14281 gcgtacggtg ccgtacgtga gcaaagccac cggtgtgccg ctgccgaagg ttgccgttgg
    14341 cctgatgtta ggcaagaagc tgcgtgatct gacggcgctc accggaggcg tgacttcggg
    14401 ccttctgcag gtgagtcagt acttcgtgaa atcgccggtg ttcccgttca acaaatttcc
    14461 gggggccgat cctgtgctcg gaccggagat gcggtccacc ggcgaggtca tgggagtagg
    14521 cgaaaacttc ggcgaggctt tcgcgaaggc gcagttgagc gccggtactc ccctgccgga
    14581 tcaaggcgct gtttttatca gcgtgaacga ccgccacaag acggaagctg cccgaatcgc
    14641 caagcaattc cgcgaagcgg gcctacgtgt ggtggcgacc cgtggcacgg ccgcggcgct
    14701 gcgtgcccag ggcgtagact gcaaaacgat tttgaaggtg aacgaaggcc gtccgaatgc
    14761 cgtcgacctg atgaaggcag gctccattca gttggtgatc tacacagcga cgggcgccca
    14821 ctcgttcacc gatgaaaaag cgatccggcg cacggctgtc gcgtaccgca taccgtgcat
    14881 cacgactatc agcggagccc gcgcggcagc cgaagccctg gagtcgcgcc gccgcgatcc
    14941 aatccgtgta tggaacctgc aggaactgca ctcgcggggc atggagccgg catcggctag
    15001 ccattcctaa gtattctgat tctgattcgg aattgcggcg acctatccga taccgcttgc
    15061 tggcgcgcgc ggctcagttc cgagccgtga ccgcaaggga gcggtcctcg ggaaaccgag
    15121 cactaagcag ggggattatc gggcttttgg gcctcgcgcc tgctcgccgc cagttcgctc
    15181 atcacgtcgg agacaagctg tttcgcgtcg ttcagcagac ggtcgatgag ttgaacgtcc
    15241 atggcgggtt tggccggatt gcgcgtgctt gtgcaataca cgccgtgctg gccaaccagc
    15301 accagcgcgt cggtgagcac atccagcgtg acggcaagcc gtccacgggc ggttcccatc
    15361 atgaactcgt actggtccag atcgtcgcgc cggtcttcgt aaatagacat agcaagaacg
    15421 ctaagacgaa tgcttcttca gccacgcatc caactggctg cgcaaccggt cccgaacggt
    15481 aatgtattcg ggattcgaat agacattccg ctcctcctcg ggatccacgc ggagggcgta
    15541 tagctcgttc gggcccttcc cctcgttgcg gatcacgagc ttgaagcggg gatcgcgcac
    15601 catggcagtg ttgcggaaat acccgaagac cacctcgttc cagggttgtc tgcgcggcag
    15661 cggcctgcct tgtgcaaggg aggcataact gcggccacag agattccgcc cggaaggcgg
    15721 tgccgttccc gtgacttcgc agatcgaagg aagaaaatcg tagaagctga cgagttccgg
    15781 acgcgcggct tctacgggaa cccggccagg ccaatgccag atcatgggca cctgcatcac
    15841 ttcctcatac atgttgatgg ggtccgaggc gagccccttg ctccacaaac cgtgccgtcc
    15901 caacaggtag ccgttgtcgc cggtaaaaac gaccagagtg ttatcctgca agccgcgttg
    15961 cttcaatcgt gcgagcagcg ccggtatctg gtcatcgagg gccgtcaccg aggcggcggc
    16021 ccggcggatg ttgcccacgg tgtcctggag catttccttt tcgcgaagcg cgttcggcgc
    16081 agcgggcagc catccgctct gcttgaaatc cgagttggca tacatgtcgt aatacttctg
    16141 cggatgtccc gtgtaagggg tgtgcggatt caggtaggca accgtcagaa aaaacggttt
    16201 gtccggattt tgtttgtcga gaaactcccc tgcccggttc gtcatcaact ccgtgaggta
    16261 gcccttttcc tcgatcatct tcccgttcag gctcatggcc ggatcctgat agctgcgtga
    16321 gcctcccagc atcgtatagg tatagtcgaa cccatgcccc ggcgactgat cctcgcccat
    16381 gtgccacttg ccgacgtagc cacagttgta tccctgcgcc gaaaggatgt ctgaaatcat
    16441 cacctctttc gcaaaagatg gaggaggcgc ggcctgaccc tgcggaggac tctcgatggg
    16501 ctttgccgtg aggaaatcat gaatgccatg ctgcatgggc gttcgcccgg tgaacagcgt
    16561 ggcacggctg gccgaacaaa tcggagtgca gacgaagctg ttcgcaaagc gtgtgccggc
    16621 gcgtgccagc aggtctatgt tcggcgtccg gatctccttg tttccgtaac aacccagcat
    16681 ccacgcagca agatcgtcgg ccagaatcag cagaacattc gggcgcggcg ccggccgctg
    16741 ttgcgcaatc agcggcgccg ctccgatcag aaaaaaacta cgacgggtta atgacacaaa
    16801 ctcatcactc ctacaggacg tccgcaaaac ctcgcttcaa atgccggaag aacagaccgc
    16861 cgatgacgaa tgccgccgcc gcgcagacgc ccatgatcgc caactcatcg atggacggca
    16921 agcggtagga aagcagccgg tcccggtagg cccggaccat gtacgccatt ggattcacac
    16981 gcatcagaaa ccggaactgt ggtggaacct gttcgatggt gataaaaatc ggcgtcatcc
    17041 agaaccatag agtcaacacg accatcacca cctgcgccgt gtctcgaata tatacctgaa
    17101 ggctcgaaac aatccagccc acaccgacgg caaacaaccc gatcaatgcc atgtacaccg
    17161 gcagtaatac gaacatcggg ctgacgtagc ccagccacac aatcgctgcc gcaatcaaaa
    17221 gcccgagagc catcagatga ctgagaagcg cagaaagaaa aatggacacg ggaacaatct
    17281 cagcgggaaa taccgtcttc gtgatcaggt tggaatgttc gagcagcgat gtggacgagc
    17341 gctgtacggt ctcctgaaac agcatccacg gcaaaaaccc gcagaacaga aacatcgtgt
    17401 aattgtcggt ggcttctccg ggaggaatcg gagcccgaag gcaaattttg aaaacaaagg
    17461 tccaactcaa cagcagcacc agagggtgaa tcaggcccca cagccacccc gccgcggaac
    17521 cgacaaaccg ctgtttgaaa tcgcggcgca caagctgaaa aagaagtgtc cgccgttcta
    17581 tcaggttgga tagaaaatgc tttcccggaa ttgccgtgct caaaaagtca ctgtagcaca
    17641 gagcaccggc aacaccggat gtcatccgaa caggcgcaac tgcctcaact ttgccggcca
    17701 cattctcaca gggagaggag cgatttcaag ttcctgcgat agccaaagct ggccgcgctt
    17761 gcgcgcgcgc gtccggcgcg taacagccag cctctgcggc gtgtcatgca aagaatggca
    17821 tcgcggacaa aggaccacga gcgaggttcg gtccgcgggg tcgtgcgaaa gatgcgccac
    17881 attcagcagc ctgtgcgggg agccgcagtt ctggcagacg ttgccggcgg cttcgagcag
    17941 cgtgagacgc aggcgccgcc accgggcatc gtagtatttc cgatactccg gtcgaattgg
    18001 catatgcggc cggtgttatc ccaatttaca gcagtatacc cgcactcgta aagaccgcct
    18061 gccgcgcgga actcatgatg tcgttcaccg catccacgaa ctgcacggct acttttggcg
    18121 cgtcaaagca ctgaacccgt gccaaccctc gctccgccag aacgcgccgc atatccggac
    18181 tgcggtagag ttgcccgatg gcggaggcaa gcgcccgtgc gtcgcccggt tccaccaggc
    18241 acgcttccgg caccacttcc ggaatcgcgg cagcacgcgc cgcgataatg ggcttacccg
    18301 cggccatggc ttcgagcagg acaatgccga acccttcctg gaggctcgga agacaaaaaa
    18361 cgtcgcagct tgtatattcg gctgccagcc gcgcctgcgt cacgtcgccc agccactcga
    18421 cggtgccttc caggcggagg gcacgcgaca gctcccgcag ccgtttcgac tccggccctc
    18481 cgcccacgat ccggaactgc aagttcccga tgcgctcccg taacagggct gcggcgcgaa
    18541 gtaagaggtc cagccgcttg cgcggataga accggcagac tgtgagaata gagaacctgt
    18601 tcgggtccgg cgcaactccg ctcccgtcga gcaactcacg ccattgggca aggtccagca
    18661 tctccggaac gacatgaacc ggctgcggga accggtacag ccgcttaagc tgctccgcgg
    18721 catatttgct cgtcgttatc actccggcgg cccgctggac atggattcgt tcgcaagccg
    18781 cctggatcga caacatcgtc cgcgtgatac cccgctcaaa gcgtagctcg tccgcaatca
    18841 cgcccttgat ggccgcaatg tgagggatag gaccacgtcc ggcgatgcga tacccgtcga
    18901 gatcgaagcc tactaccagg tcgaaggacg acaggtcttt gcgccggagg ctttcgttga
    18961 accggagcct ttcgagcgtg aaaatcctgg agtgcttctc cggagtgaca acctccaccg
    19021 gatgacccaa cccgcgcaac gcggatgcga gcacgtgaat cccgcggaac gtgccgctgc
    19081 cggcagtgac attctgtggc gtcgaggtga ggaaacagaa tttcaaagta acgttatttc
    19141 ttactctaca ctccgcccgc cggcggtgta gctgagccgc gcgcgtaagc gagcggtggg
    19201 ccggggcctc aaggcaacgt ttgcagcaca accgctccgt taccgtcgcg gctcagctac
    19261 cgtagcggcc atgttgcgcg cctgcctggc gcgcggcaag gatgccgtgt gccggcccgc
    19321 tataatacga gaagctgctt ccttccgatg gcttctgatt caaccgctct ggagattgcc
    19381 gcaagccgta aagctggtcc ggccgcccgg gctggcgtga ccgcgctggt gctgctctct
    19441 ttcggacatt ttttcatcga tctgtactcg agtgcgttag gtgcgttgca gcctttcctg
    19501 gtggataaac tcggcctgac gttgacgcaa gcggggctcc tcggcggagc gatggtattc
    19561 acctcctcgg tgatgcagcc tgtttacgga ttcctgtcgg accgctttca cacccgcctc
    19621 tttgcggcgc tggcccccgc tgtggccggc atcggaattt cggcgctcgg tttagcaccg
    19681 ggctttggat ggctgctgct gatgatcgtt attgggggtg ccggaattgc ttcctttcat
    19741 cctcaggcat cggcgcttgc aaccaccggc gtggcctctg gcaggggacg gtcgatggca
    19801 atcttcatca gcgctggaac gctggggatg gctttgggac cgacctattt ttcctgggtg
    19861 gccatcacgc tgggactggc gaacacctac tgggccgcca tacccggcat actgatgacg
    19921 attctgctgg tttcgatgct gcctcccctg caacatgggg cggccagcag gcaaggcggc
    19981 ttcgatctcg caccatttaa agcggtgtgg aagccgctga ccatcctcta tttcctggtc
    20041 ttcatccgct ccattgtgca gatcactttt gcacaattgt tgcccctgta cctgcaccgg
    20101 gagcgtggat tttcgatcgc cggcgcaagt tatgccctat cgctatatct cacggccggc
    20161 gctatcggcg gttttctcgg tgggcacctt tcagaccgtt ggggcgggcg caaggtgatc
    20221 ctgatttcca tgatttcgtg tgtccctttt ctcgcgctct tctttcttac gcatggatgg
    20281 atttcgatcg ccggccttgc aatgggcggc ctcacacttc tattcaccat tccggtgaat
    20341 gtcgtgctgg cgcagcaact cgctcccacg cagacaggca ccgtgtcggc gctgatgatg
    20401 ggcttcgcct ggggagtggc gggaattatc ttcatcccgc tcacgggcct ggcatccgac
    20461 catttcggga tgcaccccgc cctgatgagc ctgacagtgt ttccgctgat cggtttcttc
    20521 ctgacactga aactgccgaa agggatgtga gttggatcag gcgccgaagg ccgtatgtct
    20581 gctaagcggc gggctggatt cctccacctg cctcgccgtg gcgcgcgccg atcgtttcga
    20641 aacctacgct ctttccttcg actacggcca gcgccatcga gtggaactgg acgcagcgcg
    20701 gcgagtagcc gcaagtctca gcgcccgcga gcatctgatc gtccccgttg gtttgggatt
    20761 gtttggcggc tcggccctga ccggcgatat ggcggtgccg aaatcccgcc cggccgcgga
    20821 gatcgccagc ggcattccca tcacctacgt gcccgcccgc aacaccatct tcttatcgct
    20881 ggcattggcc tgggcggagg tgctcgggtc aacggacatc ttcatcgggg tgaacgccat
    20941 cgattactcc ggatatcccg attgccggcc ggagttcctc gatgccttcg agcgcgtggc
    21001 aaacctcgga accaaagcgg gagtggaggg cagcggaaaa atccggattc acgccccgtt
    21061 gaaggagcta tccaaggccg cgatcatccg tttagctatc ggtctcgggg tggatctgtc
    21121 gctgacgcat agctgctatg atcccgatag agagggcagg ccgtgcggag cctgcgattc
    21181 ctgcctcctg cggcgcaagg gcttcgcaga ggccggaacc gcggaccccc tggagtaccg
    21241 cgaatgaaaa tctcggagat cttttattcc atccaaggcg agggcatgct ggtgggaaca
    21301 ccttcggtgt ttgtccggac cagcggctgc aaccttcgct gccactggtg cgacacgccg
    21361 tacgcctcct ggaagccgga aggcgaggat ctgtacctgg gcgccatcct cgccgaagtg
    21421 cgccggtatt gggccgacta tgtcgtgatt accggcggag agccgatgat cgtcaaagag
    21481 atggtccggt tcacacaaca gttgaagacc ctgggattcc acatcacggt ggagacggcg
    21541 ggcacggtgt ttgagcccgt cgactgcgac ctcatgagca tcagtccgaa gctgtcgaat
    21601 tccacgccta cgacgataga gggcgggcgc tgggcggcgc aacacgagcg gctccgcatt
    21661 cagccggagg tactcaaaaa actcatcggc gcttatccgt accagctcaa attcgtggtc
    21721 gagaaaccgg acgacattgc ggaaatcaga tccctggtga atgacctcga cgcggaccgc
    21781 cggcgcgtcg tgctgatggc cgaaggaacg gaccccgaga ccctgcatga gcgtagcctg
    21841 tggctggcag atatctgccg tgaggagaag taccgcttca cgccgcggct ccacatcgac
    21901 ctctatggga atcgccgggg cgtgtaggcg cggcctcagc ggcgcgcgaa taagaaccgc
    21961 tctgcttccc tccaagcctc tccgagcaca ttcgtgagct cgtggttcga gtccagaacc
    22021 ttcagctcta cgttcggatg cgtggccgca aattgctcgg aataggccag cggcacaaca
    22081 tcatcctgag agccgtgcag gatcagcgtg ggctgatgga aatccggata gtcctcgtaa
    22141 cgcttaccgt cttcgatgag ctgataaccg acctcccgca tctcgccggt ggcatagtgg
    22201 tggaccagca acgcgcccga ggtttgccat tgccggaggg tggcctcgcc cagcgattca
    22261 ggccatcgat gagtgaaccc gaaggcgggc gccagcagaa ccagcttttc cgtttccgga
    22321 tgccgtgagg cgtagagggc ggcgatatag cctcccatgc tcgagccgat cagcgttacc
    22381 ggctcccgtc cggcgagccg ttccaacaat tgtagctggc cggtgatggt cagcccgcga
    22441 aagtcgcctt catccatcgc gggaaccagc aggtcgatgc cgcgcgcggc aagccgttgc
    22501 tggaagtatc gtgccttgcg cgaagcaggg ccggaagcaa aaccgtgaag gtataggatc
    22561 atttctcgaa ggggggttcc gccgaaaggc gcccgaactc ggagtacaga aacaggttct
    22621 cggccacgag cgtggaccct ctgaactggc ggtggacgac ggagacgggc accagcaccc
    22681 catggctggt tcttgtgtac tcgaccgcgc cctcatcgcg cagtttctcg ccgccgtgct
    22741 ttctttcgtt caacgtcgtg attttcaggg gaacgaaatc cggctcgcga agccagattt
    22801 cgccttgcag aatgagtttc aacgcctcgc ggttctcaaa aatcgtgagg ccgccgccat
    22861 ccttctgccg gtaactgagg acaatagcct gctccgcccc aataaactcg cggcgcacca
    22921 gccgaaaact gtagttcggg agggcggcgc gcgaaaagag cgaaatcatg ggaccgaagt
    22981 ccgtcacggc gccgctcagg ccatagcttt ccaattcgcg cagcagtttt tgtttcaggc
    23041 ggtcgtcggg agtggtgacg cccatggtca gtgccgtccg ggcctcccgg gctcggagta
    23101 tacgtctgcc atccaccgaa ataaccttgc ggagctcgtg caacacaccc ggggattcgg
    23161 cgagggaggc gtaaccgtat tccgacacga caacccgctg ctgatagacc ggaggccgcg
    23221 gccgggacct gcttgaagga agccatgtct ttaccgcgcg ctgcctcagc gtctcgcggg
    23281 caaccaggct gggagcgttc tttatgaaga tctccgcttc ctctgacagc cggttgaggg
    23341 ctgcctgaac ggcagcgccg ggagtttcgg gtgcggcggc agatcccccg gccccgaagt
    23401 agaacgcgag ggtggcggca agcagccatg cgggtaagag gaccatacgt gcctgaaacg
    23461 cttttaatat tttatccttg tgtgagttgc gtatagtgga tcgtcacggc tagaatcaat
    23521 caatttggag gattcatgtc ggttgagcag aaggtgaagc agatcattgt cgagcagttg
    23581 ggcgtggatg aaggacaggt ggacagtacc gcctcattcg tggacgacct cggcgctgac
    23641 tcgcttgaca tcgtggaact ggtgatggct tttgaagagg cattcgatct ggacatcccg
    23701 gacgaagatg ccgagaagat tgcgaccgtg aaggacgcca tcgactacat cgaagggaaa
    23761 gcggccaaga aatagcagtc ccgctttttt tctctagaag ttttttcccg tacggagcaa
    23821 attattgtca cgcagggtgg cagttactgg tgtcggactc ctaactccat taggcttagg
    23881 aacggaggag acgtgggagg cggtccggag cggcaaatcc ggtatcaccc ggatctccgc
    23941 attcgatccg tccgcctaca attgccagat tgcaggcgaa gtaaaggatt tcgacccgac
    24001 ccggtatatc gatcgtaaag aaatcaagaa gatggggcgg ttcatccagt tcgcgatcgc
    24061 cgcgacggaa ttcgccctgt ctgcctcggg cctcaagatc gacgagagta gcgcggagcg
    24121 cagtggtgtc tacatcggaa gcggcatcgg cgggttcgaa gtcatcgagc gcgagcataa
    24181 gatcttactg gaacgcggac ccggcagaat atcgccattc tttattcccg ctaccatcat
    24241 caatctggca gccggctacg tttcgattcg cacgggcgct aaggggccca attccgcgac
    24301 ggccaccgct tgtacttcca gcgctcatgc cgtgggcgat tctttcaaaa tcatccaaag
    24361 gggcgacgcg gacgtcatga tctgcggggg tgccgaggcg cccatcaccg agatgggcat
    24421 tggcggtttc gctgccatgc gcgccctatc ccaacgcaac gacgagcccg gccgcgcttc
    24481 gcggccctgg gatcgcgatc gggacggctt cgtagtaggt gaaggcgcgg gcattctgat
    24541 cctcgaagag ctcgaattcg cgcgcaagcg cggcgcgaat attctggcgg agatcgtcgg
    24601 ctacggcatg accgcggacg cctttcacat gaccgcaccg tcggaagacg gagacggcgc
    24661 attccgggtc atgcggctcg cgctaaaaga tgcgggactt cagcccgaac agatcggcta
    24721 catcaatgcg cacggcacgt ccacacccct cggggacaag ctggaaacgc ttgccattaa
    24781 gcgcgccttc ggagagtatg cccggaaatt agcggtcagt tccaccaagt cgatgatggg
    24841 acgcctgctc ggcggcgcgg gaggagtgga ggcgggaatt acgattcttg ccatgcgcga
    24901 tgggatgttg ccgcctacca tcaactacga atgccccgac ccggaatgcg acctggacta
    24961 tattcccaat gaagcgcgcc cggctcaggt cgactatgcg ctctcgaatt cgtttggatt
    25021 tggcggcacc aacggttgta tcatcttcaa gcgctaccgc gggtaagcgc ccatgaaaat
    25081 catcgttgcc atcaagcagg tcccggccag ggattccgtg ctccaggtgg actcttcggg
    25141 caagtggatt cgtgagtcgg atctgagctt cgaggtcaac gagccggatg cttacgccct
    25201 cgaagaagcg ctccaattga aagagcgata tgggggagag gtgatcgcgc tatgcgccgg
    25261 tcccgcgcga gtgtcgcaag ccgttcggga agcgctggca aaaggcgccg accgcgccat
    25321 tcagatcgag tcagacgacc tcggtgcggc cgattccctg ggcatcgccc ggctgctggc
    25381 ttccgccatc aaggccgaac agccggacct ggtgttgacg ggcctgcaat cggatgacct
    25441 cggatacgga cagacgggtg tcatcatggc cgagctgttg ggatttccgc actcgacgat
    25501 catcatgcag gtcgaggcgg ccggtagttc cgttcgcgta aagcgggagc tcgaaaatgg
    25561 ctggttccag tggatggaaa taccgctgcc ggccgtattg acgattcaat cgggcatcag
    25621 caagctgcgg tacgccacgc tgatggggat caagaaagcc agaaccaaag aggtgaagcg
    25681 tttgaccgcg gccgagttgg gcgggctcgc ggcgccggtg ctggcgttag agaaggtttg
    25741 tcttcctcaa aagacgaaag agacgcagat gctcgacgga accccggcgg aggctgccgc
    25801 ggcgctggtg gagaagctgc gctttgaggc acgtgtccta tgaacgtgct ggtggttttg
    25861 gagcagcacg gcggcgagtg gaaccgcatg tctttcgaag ctctgtccgc ggggcagcag
    25921 atcgcggcag acatgggtgg ccgtgcttcc gccgcagtac tcgggaacgg gctgtcgaag
    25981 ctggccgacg aattgcgtgg caccgatctc gaaacagtct acgcagtgga gcatccgctg
    26041 cttgagaact acactaccga tgggtacacc gccactctgg aagctcttct gaaaacgagg
    26101 aagccgtccc tggtgttatt cccgcacacc taccaggtac gcgacttcgc tcccaaactc
    26161 gctacgcgat tcaaccaggt gctggtaagc gacatgatcg ggcaccgtgt cgaatccgga
    26221 tctttaattc tggtgcggca gtattttcag ggcaaggtga atggcgatct gcgggcattg
    26281 ggagacgggc cgcacttcgg ctcgatacag gccggagcgt accgctcaga tcgcgtggcg
    26341 ggcggcagtg caactgtcga ggcgttcact cccgatttga acgccacttc cattcgcaac
    26401 aaacccgagc cgcccttccg cgaggcagag cgcgcggtag atctcactac cgccgatatc
    26461 attgtgtcgg taggacgcgg catcaaagaa aaagataacc ttcctttggt ggaagagctc
    26521 gcaaaaacct taaacgcgga attggccgct tcccggccca tctgcgataa cggatggctt
    26581 cccatggagc ggcaggtggg cagttccgga cagaccgttt cccccaagct ttatatcgcc
    26641 gtcggtatct ctggcgccat tcagcacctg gtgggcatga agggcgccag aacgattgtc
    26701 gccatcaaca aggatgaaaa tgctcccatc ttcgaagtgg cggattacgg tattcaggga
    26761 gacctcttcg aagtagttcc cgcgctggta aaagaactca aaaagattcg ttagtattaa
    26821 cacgcgattt tccgattaac atcccttcag cgttgacatt caccggaaat tgcggttcac
    26881 ttgacctcat aaaccagtcg cggctcagag gcgcggcccg gcattgaggt tcctatgtcc
    26941 agactgaatg ctttcacact attgctcgca ggactttcgg ccttcgccat cacttcaact
    27001 gcaaatgcac aggtgactgg caccatcagt ggtttcgtga cagatccgac aggtgcggcc
    27061 attccgggcg ccaggatcac cgcaacactg attgaacaga atgtttcccg cacggcggaa
    27121 tcgaatgatt tgggctacta cgtattcaat gcgctgcctc ccggtcttta cgcgctggcg
    27181 ggcgaaaaag aaggatttca acggctggtt cgaacggaga tttcgctggc cgtaaaccag
    27241 aatctacgtg tcgatcttgg cctcaaactg gggcagctcg cagaatcggt ggaagtaacg
    27301 gctacggctc cgctcgtcga cacacgctcg ccggccctgt ccggtctggt ggatgaccgg
    27361 cgcgtggtgg acctcccact gaacggacgc aacgtcatct cactggcggc gaccctgccg
    27421 ggaattctga gtgttgtcgc accgcaacaa ctgtccgatt cgcgcagcgg accgctgatg
    27481 aatgtcaacg gaagcgtcac aacgcagaac atgttcacct tcaatggggg catcttcgtt
    27541 catccttcac ggaacaccgg attgaactat ccgcctccgg acgctttgcg cgaattcagc
    27601 attcagacgc agagtttctc ggcggagtac ggacgcaacg ccggctcgca ggtgaacgtt
    27661 gtgtcgaagt ccggcaccaa cgaatttcac ggctccgcct gggagttctt gagaaacaac
    27721 cacctcaacg cccgcaactt cttcgcttcg cgggttccca ccaagatcca gaatcagttt
    27781 ggtcttgccg ccggaggccc tatcaggaaa gattcgatct tcattttcgg ttcttatcaa
    27841 gggctgcgag atcgcgccga agccgtcgga gtgtcggcgg atgtaccttc gcccgcgcaa
    27901 cgcaccggag atttcaccgg cttggggcgg actttgacga atccggtgga tacactcacc
    27961 gggcagcctt ttacggaccc atccgggaga ccctgcgttt ccggcaacgt catcagcccg
    28021 ggatgcatca gccccgtggc acaaaacctc ttgccgctga taccgctctc gcctacg
//
LOCUS       DASTMK010000616        12302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_287541, whole genome shotgun sequence.
ACCESSION   DASTMK010000616 DASTMK010000000
VERSION     DASTMK010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287541"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..137
                     /gene="rrf"
                     /locus_tag="VFQ79_25495"
     rRNA            21..137
                     /gene="rrf"
                     /locus_tag="VFQ79_25495"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            293..1483
                     /gene="fdhA"
                     /locus_tag="VFQ79_25500"
     CDS             293..1483
                     /gene="fdhA"
                     /locus_tag="VFQ79_25500"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HEU0143102.1"
                     /translation="MASNRGVAYMSPGKVEVQSIDYPKLERGNRKCEHGVIIKIVATN
                     ICGSDQHMVRGRTTAPAGLILGHEITGEVVEAGRDVEFIKVGDLVSVPFNIACGRCRN
                     CKEGRTGICLNVNPARPGAAYGYVDMGGWVGGQAEYIMSPYADFNLLKFPDKDQAMAK
                     IRDLTLLSDIFPTGYHGAVTAGVGPGSIVYVAGAGPVGLACAEACHLLGAAVVIVGDL
                     IPERLAQARSFGCETADLKKDATLSEQIEQIVGVPEVDSAVDCVGFEARGHGQDAAVE
                     KPATVLNSLMQVTRAGGAIGIPGLYVTDDPGGVDESAKTGNLSIRIGLGWAKSHSFAT
                     GQCPVMRYHRQLMQAILHDRVQIAKAVNVTVISLDEAPQGYKDFDKGAAKKFVIDPHR
                     LVAA"
     gene            complement(1505..1804)
                     /locus_tag="VFQ79_25505"
     CDS             complement(1505..1804)
                     /locus_tag="VFQ79_25505"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0143103.1"
                     /translation="MSEKRRGPRQVSSHDLEVAWGDAGGVLHRIRAICVDVSEGGMRL
                     KSTVKLPLHENVHFKFKKIRFSGTARIRQVTWRGTYYIFGAEFTGGLKWRDAARD"
     gene            complement(1865..2917)
                     /locus_tag="VFQ79_25510"
     CDS             complement(1865..2917)
                     /locus_tag="VFQ79_25510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0143104.1"
                     /translation="MKNQKIAFAAAGLVAAGIVSAVVSRRRRSGPRLAGQVALITGSS
                     RGLGFQLAREFAREGCRVVICARDGPELERAWQDLAGRGAEVLALLCDVSNRADVEEL
                     FERVRSEWGGVDILVNNAGIIQVGPIESMRVEDFEQTMAIMFWGTLYPTLAVLPEMLE
                     RKSGRIVNITSIGAKVSVPHLVPYNCAKSAALALSEGLRAEVAGKGVHVISIVPGLMR
                     TGSYQQALFKGKQQDEYEWFSLGATLPGISMSAERAARQIVEATIRGHTERILSTPAN
                     LLARFKGLFPSLTTKILALVNAMVLPEGEGTEQGDPSRGKEVHTGIRSQVLDLIHTLG
                     RLAGERLNQEPVTSGR"
     gene            3050..4078
                     /locus_tag="VFQ79_25515"
     CDS             3050..4078
                     /locus_tag="VFQ79_25515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0143105.1"
                     /translation="MLIAFMEDKTGNMNIRAISRAIVREFRRMADMNPKALISLLWTT
                     VSEWMNDNAGRMAAALSYYSLLSTAPLLVVVIAVAGLAYGEGAVRGQIFWQLQDLIGA
                     EGAKAVEMMLKGAQKPATGIFASILGLITLFFGASWVVAELKSALNTIWKVAPPQGES
                     TLGSVFTMLRNRVFSFALVMGIGFLLIVSLVVSAWLAAIGEFFQQWLPVPAALLTFAN
                     FLISFVVITFLFTLIYRILPDTRIAWGDVVVGATVTSLLFSFGKYLIGLYLGRSSFTS
                     TYGAVGSLVIFMVWVYYSAQIFFLGAEFTKVYAAKHGSKQVRKTREKLVPTESQGPPA
                     AGELSPNR"
     gene            complement(4086..5831)
                     /locus_tag="VFQ79_25520"
     CDS             complement(4086..5831)
                     /locus_tag="VFQ79_25520"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0143106.1"
                     /translation="MGALAALLVFIGAACLAGQVPPPIPTDRPGPRSAGKGATLEGRT
                     ENDNGATLPHVMLTLRGGGAGQMFKYTARSDAQGRFVFEQVEPGQYTLTAEKPGFVRQ
                     SAGGRRGGGSTVSLTASEDTPIKDIVLRLVPQGVISGRVTDENGEPVERVSVLALQWR
                     YLDGKRLLRPATGAITDDLGAYRIRGLAPGSYYIAARSDQLLRAGVFVQYGDAPAASV
                     PEGREEAHQTTYYPGSIDPAGASPLLVAPGADLGSIDIRFLKAPVFRVRGEVIDDVTG
                     RPVADFWVSLTPVSPVNVISMSSVGGPRISGGKFEVLAVSPGAYYLIAYVTGPGETRY
                     ARQPVFVTDQNVTDLRIHIPRVVEVSGSVRLEPSGGQASEQSPSSQQKPTSPDAVRIY
                     LQPVEGFGVGETPVASAAAGGRFVMRGVVPDKYRVAVTGLPAGSYLKSILVAGQERPD
                     GSADIAGSAPIEIVLASGAAQLTGSVTDSEGKPASDVELTLFPESPERQRRRDLFRVT
                     TSDQDGQFRFTDVAPGDYKVLAWQNLEAGAIQDPEFRRQFEAKAKSVSLGVNGQESVE
                     LKEIPAEEIARVLGR"
     gene            complement(5887..6873)
                     /locus_tag="VFQ79_25525"
     CDS             complement(5887..6873)
                     /locus_tag="VFQ79_25525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0143107.1"
                     /translation="MHRFVPLIRHPDLLTVVAHFWPRPLDERRFPVQKNLYRTDEETQ
                     VLVEEQSAGVAPSGHLVLIHGLESSSRAGYMKSLASAALHAGFSTHRMNLRSCGGTES
                     LCRTAYHSGLTADLEGIVRELATSTGQPVFLAGFSLGGNVALKLAGELGERARGLIAG
                     VCGVSTPIDLAVCVRRMEEPRNRLYEVRFLRSLRRRMRVKHQCDPARFPLDGIDRVRS
                     VREFDDRFTAPAFDFRDAAEYYETQSSIRFLDRVRVPVLLIQAKDDPVVPFEPFTRSA
                     VTANPNIHLLATEYGGHVGFLSRRQPRFWSDAVILDWMQALVRGTSRPQFQY"
     gene            6972..8537
                     /locus_tag="VFQ79_25530"
     CDS             6972..8537
                     /locus_tag="VFQ79_25530"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0143108.1"
                     /translation="MPLLPSQDAGKENASIRGTVKEFPGGAPAPEVSIRLHGSTMSRE
                     VSTDSSGRYELTDIHPGNYSLSTHGAHYGRTPLGVLAPIKRVRLVPGQDMTVDFEVKK
                     EAGIAGRVLDVDKNPVPNARIQVWARIFIGGSPSFGQCGSARTNDLGEFRVSGLADGR
                     YYISAGIRLLPFRKRPAGAKQDTKPKLADVGVFYPSGLSVVSAAPIDLGAGEQREGID
                     LALPRMETYCLSGSVAAGAASSGNLGLIAFDGGWRGYIAQGAFRGNEEFEVCGIPPGF
                     YHLEASTSIDGTHLSFGRAEVMIGRRHESVGPVHLVSGVKVPGKVIIVGANPPEPLPK
                     GIRVSLTPIQGRLIRKGEEISAQVDTAGDFTIPNVFPDERWLEVRQLPPRYYVKEARM
                     GAQDARYEPIRPGSSELTIVLAPDGASVSGTVAEQDGVVPPEVIVVLAPSPLPQTGAP
                     GLVRTQWADQAGGFAFDTVVPGEYFLLAFMGLLPGEGENLDFLRAHLSKAEELTLKPR
                     DRKSANLKVLNAH"
     gene            complement(8531..10039)
                     /locus_tag="VFQ79_25535"
     CDS             complement(8531..10039)
                     /locus_tag="VFQ79_25535"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0143109.1"
                     /translation="MRRPKAEGRKQPVMALCLLLAAMPKAAPAEELTCRVIQDNSGTP
                     VASAEVRIERSPGGTAVVDTVTDGEGRFRAPGLIAGEYLITVEKPGYMKTRIAARIGR
                     EQTAITIRLVRHGAITGRVMDQASQPIRGARVTAMSRNADGTLQAVEGVGTAASVDSN
                     GLYRLYGLPPGEYAVAVSGRSVGFLTYPGNAQPAFFPVAGGEEHRGVDFNITQRSLFS
                     VVGKVDLPAPGEVFSVALAPVEQPGIAIGSAQTSGDGSFRIDGVPQGSYHLFAAGPVK
                     GTAAKGAILGSEPMFGRMRIDLAGDHVTDIRLPVGASLRGALQLQYEHGGADACPKTS
                     TITATSLDAWPAMLLRSVVAAFDHEVEIPYLALGRHQITAVELGATCHQAAGAVLDVT
                     AGVPAKAVIRLAAAGSIRGVLTGSAEGCTVVLLAVKPVQDRDAIQIAIPDASGRFAFG
                     GLAPGDYRIGVRRGTEPHQARWVLKNEWLIELQVPGGAPTDVELPVPPGGPQ"
     gene            complement(10049..11182)
                     /locus_tag="VFQ79_25540"
     CDS             complement(10049..11182)
                     /locus_tag="VFQ79_25540"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU0143110.1"
                     /translation="MTMSVTRRAALASLAALSKAGASSARKLPPDTPAAVAAILDRSL
                     EQDPSVWNTDWFGTLLMKGMLEWGGSHVPEVRQYARRWLDHHLSLTSVSKYSGAKSRT
                     VIAGGIPITTYAGHYGLALPCYEIYRQLQDERARRIAVDVASIILHRSARNHLGLVSH
                     DDYSEFAIPDTCYFVASALMIASRLDPRNETAFREQAVFQLRAYTDTFLVKETGLAKT
                     ILFNKTGLGNTYWTRASGWLLWAMAGVLRHLPASDTAYRPFLEDMRRLAEGIVRVQHP
                     EGGFRVLLDEPDTPLETTGSAMFASGLHEAVRSGWLPGSFKEPATRAWQYVQKNLTPD
                     GDIRQAYTGWAVPAEKREMSMDKHVMGWIPGFILSTAYEMSLG"
     gene            complement(11179..>12302)
                     /gene="alr"
                     /locus_tag="VFQ79_25545"
     CDS             complement(11179..>12302)
                     /gene="alr"
                     /locus_tag="VFQ79_25545"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0143111.1"
                     /translation="FALRSWTEVSRRQITENFRAVRNVVGASVEVMPVVKADAYHHGA
                     VEVSRLLVSEGAQWLAVSSVEEGASLREAGIRTRILVMADFLPAERAGLIEYELTPVI
                     HSLPDLREWDQLAVRSGTPLHYHLKIDTGMGRLGARAGAAEILDAVNGARHCRLEGLM
                     THFASAGDYSTSQTEEQTATFLDIVSDLRRAGVKPPYLHLASTIPVAYGRSRAWGQLV
                     RPGHAIYGYIPPPRGDVPERILDVKPALTWKASILTVKDLPEGSLVGYGGTYRTSRPT
                     RIAVVAAGYADGVPHQLSNKGKLIASGKLVPILGAVSMDVTTIDVTDCSPLAPGDSVT
                     ILGREGDASLDAQDIANVAGTISYSVLCNISARVRTIYV"
BASE COUNT         2418 a         3719 c         3734 g         2431 t
ORIGIN      
        1 tattctgttt catccccctt ttgggtgact ctagcgagga ggtcacaccc gttcccatcc
       61 cgaacacggc agttaagctc ctcagccccg atggtactgc atgcgcaagt gtgtgggaga
      121 gtaggacgtt gcccgagtaa aaccaaaaaa gccccgtgta ctcttcatcg agtacacggg
      181 gcttttggtg tttatgggag aacccccgcc gggccccagc catttgacac catggctcgc
      241 ttttgatgag atgaagtccc atcagttcca tcaaatagcg gaggacaaga atatggcatc
      301 caacagaggg gtcgcctata tgagcccggg caaagtggaa gtacaatcca tcgattatcc
      361 gaagctcgag cgaggcaacc gtaaatgcga gcacggagtg atcatcaaga tcgttgccac
      421 aaatatctgt ggaagcgatc agcacatggt acgcggccgg accaccgctc cggcagggct
      481 gattctgggc catgaaatca ccggcgaagt ggtagaggcg ggccgcgacg tggagttcat
      541 caaagtggga gatctggtct cggtaccgtt caatatcgct tgcgggcgat gccggaactg
      601 caaggagggc agaacaggca tctgcctgaa cgtcaatccg gcgcgtccgg gcgccgctta
      661 cggctacgtc gatatgggcg gctgggtcgg tggtcaagcc gagtacatca tgtcgcccta
      721 cgccgatttc aacctgctga aatttccgga caaggaccag gccatggcta agatccggga
      781 tttgacgctg ctctcagaca tttttcccac cggctaccac ggggctgtca ccgcaggagt
      841 cggtccgggt tccatcgtgt acgtcgccgg cgcgggacct gtcgggttgg cgtgcgccga
      901 ggcctgccat ctgctgggtg cagcggtagt gattgtcggg gatctgattc cggaacggct
      961 ggcacaggcg cgcagtttcg gttgcgaaac cgcagatctg aagaaggacg ccacgctctc
     1021 cgaacagatc gaacagattg ttggcgttcc cgaggtcgac tcggcggtcg attgtgtagg
     1081 gtttgaagcg cgcgggcacg gtcaggatgc ggccgtcgag aaaccagcca cggtgctgaa
     1141 ctcgctgatg caggtcactc gtgccggcgg ggcaatcggc attccgggac tttacgtcac
     1201 cgacgacccg ggtggagtgg atgagagcgc caagaccggc aacctgagca ttcggattgg
     1261 actcggctgg gcaaaatccc actcgttcgc caccgggcag tgccccgtca tgcgttatca
     1321 ccggcaactc atgcaggcga tactccatga ccgcgttcag atcgcgaagg cggtgaacgt
     1381 aacggtgatc tcactcgacg aagcgccgca aggctacaag gatttcgaca agggcgcagc
     1441 caagaagttt gtcatcgacc cgcatcgcct cgtggcggcg tagtcggttt gaatccggcc
     1501 gtactcaatc gcgagcggca tctctccatt tgagaccgcc ggtgaactcc gcgccgaaga
     1561 tataataagt tccccgccat gtgacctgcc ggatgcgggc ggtgccggag aaccttatct
     1621 ttttgaattt aaaatggaca ttttcgtgta ggggaagttt caccgtcgat ttgagccgca
     1681 tgcctccttc cgagacgtcg acgcaaatgg ctcgtatgcg atggagaacg ccaccggcgt
     1741 ccccccaggc gacttcgaga tcgtgggacg aaacttgtct gggaccacga cgtttctccg
     1801 acatggatga aaaacaacct caccaaaaca tatcggatcg ccgcggcgat acttgaccgc
     1861 ggtgttacct gccggatgta accggttcct ggttgaggcg ctcgcctgct aacctgccga
     1921 gggtatggat caggtccaga acctgcgacc ggatgccggt gtgcacctct ttgccgcgcg
     1981 acgggtcacc ctgctctgtt ccctcgcctt ccggcaggac catggcattg accagcgcca
     2041 gaattttggt tgtcaaggat gggaacaggc ctttgaaccg cgccagcagg ttcgcgggtg
     2101 tggagaggat tcgttccgtg tggccgcgga ttgttgcctc cacgatttgg cgcgccgctc
     2161 gttcggcgct catcgagata cccgggaggg tagcgccaag gctgaaccac tcgtattcat
     2221 cctgttgttt gcctttaaaa agcgcctgct ggtaggagcc ggttcgcatc agccccggca
     2281 caatgctgat gacgtggacg cctttgcccg cgacttccgc cctcaagcct tcggatagcg
     2341 ccagggcggc ggactttgcg cagttgtacg gtacaaggtg aggaacgctg acctttgctc
     2401 ctatcgaagt gatgttgacg atgcgcccgg acttgcgctc gagcatctcg ggaagcaccg
     2461 cgagggtagg atacagggtc ccccagaaca taatggccat ggtctgttcg aaatcctcga
     2521 cccgcatgga ttcgatgggg cctacctgaa ttatgccggc gttgttcaca aggatgtcga
     2581 cgccgcccca ctcggatcgt actcgttcga acagttcctc gacgtccgcc cggttcgaaa
     2641 catcgcagag gagggcgagc acctcggcgc cgcgtccggc cagatcctgc cacgcgcgtt
     2701 ctagttccgg tccgtcgcgc gcgcagatca ccacccggca accctcgcgt gcaaattccc
     2761 tggccagttg aaagccgaga ccacgggagc tgcccgtgat cagggctacc tggccggcca
     2821 gccttggacc ggagcggcgg cggcgcgaca ccacagcgct cacaattccg gcggcaacca
     2881 gaccggcggc ggcaaaagcg atcttctgat ttttcatgga cgtggcgaac ttactcacga
     2941 gtgagacgat gctggtgcac tattgtttcg gtcatcccgg ggcgtgaact actcccgaaa
     3001 cagttgtttg cccgccacgt ctcagctaac gaggggctat gagccaatcg tgctgatagc
     3061 gtttatggaa gataagacgg gcaacatgaa catacgcgcc atctccagag caattgtccg
     3121 ggagtttcga cgtatggcgg acatgaaccc gaaggcgctt atttcactgt tgtggacaac
     3181 ggtctccgaa tggatgaatg acaacgccgg cagaatggcc gccgcgttgt cctactacag
     3241 ccttctgtcc actgcgccgc tcctggttgt ggtcatcgcg gtggccgggc tcgcgtacgg
     3301 tgagggcgcg gttcggggac agattttctg gcagcttcag gacctgattg gcgcggaagg
     3361 cgcgaaagcc gtcgagatga tgctgaaggg tgcgcagaag cccgctaccg gaatcttcgc
     3421 ttccatactc ggattgatca cactattttt cggcgcctcc tgggtggtgg cggagctgaa
     3481 aagcgcatta aacactatct ggaaagtagc gcctccgcag ggggaatcga cactgggcag
     3541 cgtgttcacc atgctgcgca acagagtatt ctctttcgct ctggtgatgg ggatcgggtt
     3601 tctgctgatc gtctcgctgg tggtcagcgc ctggctggcc gccatcgggg aatttttcca
     3661 acagtggctg ccggttccgg cggcgttgct gacattcgcg aacttcctga tttccttcgt
     3721 ggtgattacg ttcctcttca cgttgattta ccggattctg ccggacacgc ggatcgcctg
     3781 gggcgatgtc gtagtaggcg caacggtgac gtcgctgctg ttcagcttcg gcaaatacct
     3841 catcggcctt tacctgggac ggagcagttt cacctcaacc tacggagccg tcggctcgct
     3901 ggtgattttc atggtgtggg tttattactc ggctcagatc tttttcctgg gcgccgagtt
     3961 tacgaaagtc tacgcggcca agcacggatc caagcaggtg cggaagaccc gtgagaaatt
     4021 agtaccgacg gaatcccagg ggccgcccgc ggccggagag ctgagcccga accggtaacg
     4081 gaatgtcagc gcccaagcac cctggcgatc tcctccgccg ggatttcttt tagctcgacg
     4141 ctctcctgac cgttgacgcc caaagagaca cttttcgctt tcgcctcgaa ttgccgccgg
     4201 aactcggggt cctgtatggc gccagcttca aggttctgcc aagccagcac tttgtagtct
     4261 cccggagcaa cgtccgtgaa ccggaactga ccgtcctggt ccgacgtggt gacacgaaac
     4321 aggtctctcc tgcgctgtct ttcggggctt tcagggaaca gcgtaagctc cacgtccgaa
     4381 gcgggcttgc cttccgagtc cgttacggac ccggtcagtt gggcggctcc tgaggcgagc
     4441 acaatttcga tgggtgcgct gccggcgatg tccgcactcc catccggccg ctcctgacct
     4501 gccaccagga tggacttcag gtaagatccg gcaggcaggc cggtgacggc tacgcgatac
     4561 ttgtcgggca caacccctcg catgacaaac cggccgccgg cggcggcgct ggcaacaggc
     4621 gtttcaccca cgccaaatcc ctcgacgggt tgcaagtaga tccgtacagc atccggagat
     4681 gtcggcttct gctgtgacga aggagactgt tcggatgcct ggccgccgga cggctcgagc
     4741 cggacgctcc cggatacctc cactactcgc gggatatgga tgcgcaaatc ggtaacattc
     4801 tgatccgtta cgaacaccgg ctgccgcgcg tatcgcgtct ctccggggcc agtgacatac
     4861 gctatcaaat agtaggcgcc cggcgataca gccagtacct caaacttccc tccggaaata
     4921 cgcgggccgc caacactgct catgctgatc acattgactg gcgaaacggg tgtcagggat
     4981 acccagaaat cggccacagg cctgcccgtg acgtcgtcaa tcacttcgcc gcgcacccgg
     5041 aagacaggtg ctttcagaaa ccggatatcg atactcccca gatccgctcc gggagcgacg
     5101 agaagaggag aggcgccagc cgggtcgatc gaccccggat aatacgtagt ctggtgagct
     5161 tcctcgcgcc cttcagggac cgacgctgct ggggcgtccc cgtattgaac gaacacgcca
     5221 gcgcgtagca gttggtcgga gcgggccgcg atgtagtagc tgccaggcgc cagtcctcga
     5281 atccggtacg cacccaagtc gtcggtgatc gccccggtcg ccggccggag caggcgcttg
     5341 ccgtcgagat atcgccattg caacgcaagc acagacaccc tctcgaccgg ctcgccattt
     5401 tcatccgtca cccgcccgga gattacgccc tgcggcacaa gccttagcac gatatccttg
     5461 atgggcgtgt cttcggaggc ggtcaaggaa acagtgctgc cgcctccgcg gcgccctcca
     5521 gcgctttgtc tcacgaatcc gggcttttcg gccgtaagtg tgtactgccc cggctcgacc
     5581 tgctcgaaga caaagcgccc ctgtgcatcg ctcctcgcgg tgtacttaaa catttgaccc
     5641 gcacccccgc cgcgcaacgt caacatcaca tgggggagag tggcaccgtt gtcgttctcg
     5701 gtccgccctt caagcgtcgc tcctttgccg gcgctacgcg gccccggcct gtctgtggga
     5761 atgggaggag gaacctgtcc cgccagacag gctgctccta taaatacaag caatgccgct
     5821 agtgcgccca tagcaccgct ccttactaat acaacaggca tagtttatgg ccaggttggg
     5881 ttcaaatcaa tattggaatt gcggccggga tgttcctcgc accagcgctt gcatccaatc
     5941 gaggattacc gcgtcgctcc agaagcgcgg ctgccggcgg gagaggaatc ccacgtgccc
     6001 tccgtattcg gtcgccagca agtggatatt cggattggcc gtaaccgccg aacgggtgaa
     6061 cggttcgaaa ggcacgaccg ggtcatcttt ggcctgaatc aacagaacag ggacccgtac
     6121 ccggtcgaga aagcggatgg acgattgcgt ttcgtagtat tcggcggcat cgcgaaagtc
     6181 gaaggccggc gcggtgaacc ggtcgtcgaa ctcgcgtacg gagcggaccc gatcgattcc
     6241 atccaacggg aagcgggctg gatcgcactg gtgcttcact cgcatccggc gcctcaggct
     6301 gcgaaggaag cgcacttcat agaggcggtt gcgaggttct tccattcttc gaacacagac
     6361 cgcaaggtcg atgggtgtgg aaacgccgca gaccccggcg atcagaccgc gagcccgttc
     6421 gcccagctcc cccgccagtt ttagcgcgac gtttccgccg agcgagaacc cggcaaggaa
     6481 aaccggctgc ccagtagagg ttgccaattc gcggacaatt ccttcgaggt cggcagtgag
     6541 cccggagtga taagcggtgc ggcaaagcga ctccgttccc ccgcagctcc gcaagttcat
     6601 gcgatgcgtc gaaaagccgg catggagtgc cgccgaagcc aggctcttca tataccccgc
     6661 gcggctggaa ctttcgaggc cgtggatcag aacaagatgg cccgaaggag caactcccgc
     6721 ggactgttcc tcaacgagaa cctgcgtctc ttcatccgtt ctataaaggt ttttctggac
     6781 gggaaagcgc cgctcatcaa gcggcctggg ccagaaatgc gccacaaccg tcagcagatc
     6841 ggggtggcgt atcagtggaa cgaagcggtg caatccttat agtttagagt aagttcatcg
     6901 gcggtacagt gatagcatct atggtgcgtc gcaaaatacc ggttcggccc gccgtgctaa
     6961 tcctggctgt attgccactt ctgccgtcac aggatgccgg caaggagaac gcctcgatcc
     7021 gcggcaccgt taaggagttt ccaggcggcg caccggcgcc cgaagtcagc atccgactgc
     7081 acgggagcac aatgtccagg gaggtaagca cggattcgag cggccggtac gaattgaccg
     7141 atatccaccc gggcaattac tctctttcta ctcacggcgc gcactatggc agaacacccc
     7201 tgggtgttct cgccccgatc aagcgggtgc gcctcgtccc gggtcaggat atgaccgtcg
     7261 atttcgaggt gaagaaggaa gccggaatcg ccgggcgggt cctcgatgtt gacaaaaacc
     7321 cggtgcccaa cgcacggatt caggtctggg cgcgtatctt catcggtggc tccccatctt
     7381 ttggacaatg cggatcggcg cgcaccaacg atctgggaga gtttcgcgtt tccggcctgg
     7441 cggatggccg atactacatc agtgcgggga tcaggttgct tccattccgg aaacggcccg
     7501 ccggcgcaaa gcaggacacc aagccgaagc ttgcagacgt aggggttttc tatccatccg
     7561 gtctgtcggt tgtttccgca gcacccatcg atctgggtgc aggcgagcag cgggaaggca
     7621 ttgatctggc gctccccaga atggaaacct attgcctcag cggctcggtg gcggcgggtg
     7681 cggcaagcag cggcaatttg gggctgattg cattcgatgg cgggtggcgt ggctatatcg
     7741 cacagggtgc gttccggggg aacgaagaat tcgaagtttg cggcatcccg ccgggtttct
     7801 accatctgga agcttccact agcatcgacg gcacccacct ctctttcgga agagccgagg
     7861 tcatgatcgg gagacgccac gagtcagttg gcccggtcca cctcgtcagc ggcgtgaaag
     7921 tcccaggaaa ggtgatcatc gtgggcgcga atcctcccga accgctcccg aaagggattc
     7981 gtgtctctct gactccaata caaggacgcc taatccggaa aggggaggag atcagcgcgc
     8041 aggttgacac cgctggcgat tttacgattc caaacgtctt tccggacgag cgctggttgg
     8101 aagttcggca gctcccgccg cgctattacg tgaaggaagc cagaatgggc gcgcaggacg
     8161 ccagatacga gcccatacgg cctggttcga gcgagttgac gatcgtgctt gcgccggatg
     8221 gtgcatccgt atccgggacg gtggccgaac aggacggcgt cgtaccgccc gaggtcattg
     8281 tggttctcgc accgtcccct ctaccgcaaa ccggggcgcc gggccttgtc cggacgcagt
     8341 gggctgacca ggccggcggc ttcgcgttcg acaccgttgt gccaggcgaa tacttccttc
     8401 tggcctttat gggtctgttg cccggagagg gcgagaatct cgactttctt agggcgcatc
     8461 tgtctaaagc ggaagaactc actttgaagc cgcgcgacag gaagtcagcg aacctcaagg
     8521 tactgaacgc tcattgaggg ccgcccggcg gaaccggcag ttccacgtcg gtaggtgctc
     8581 ccccaggtac ttgcagttcg atcagccact catttttcaa aacccaacgc gcctggtgcg
     8641 gctccgttcc ccgccggacc cctatgcgat aatcgccagg cgccagtcct ccgaatgcaa
     8701 agcggccact cgcgtccggg atggcaatct gaatcgcatc ccggtcctgt accggtttca
     8761 cagccagcaa aaccaccgtg catccctccg cgcttcctgt aaggacaccg cgaatggagc
     8821 cagcggccgc caggcggatc accgcctttg ccggaacgcc ggcggtaacg tcgagcaccg
     8881 caccggctgc ctggtggcac gtcgctccca gttcgacggc ggtgatctgg tgccttccga
     8941 gtgcgaggta cggaatttca acctcatggt cgaatgccgc tacgacgctc cgcaggagca
     9001 tggccggcca tgcgtcaagg ctcgtcgccg ttatcgtgct cgtctttggg caagcgtccg
     9061 ctccgccgtg ttcatactgc aactggagcg cacctcgcag gcttgcccct acaggcagtc
     9121 tgatatccgt cacgtggtcg cccgctaaat cgatccgcat gcgtccgaac atcggttccg
     9181 atcccagaat tgcgcctttc gctgcggttc cctttaccgg ccccgccgcg aacagatggt
     9241 aagaaccctg cggtactccg tcgatccgga aactgccatc tccggaggtc tgggcggatc
     9301 cgattgcgat tcccggctgc tctaccggcg cgagagccac agagaacacc tcgcctggcg
     9361 caggcagatc caccttcccc acgacgctga acaacgaacg ctgcgtgatg ttgaaatcta
     9421 caccgcgatg ttcctcacca ccggcgacgg ggaagaatgc aggttgcgcg ttgcccgggt
     9481 aagtaaggaa gcccacgctg cggcctgaca cagctactgc atattctccg ggtgggaggc
     9541 cgtagagccg gtagaggcca ttgctgtcca cggaggcagc ggtgccgacg ccctcgacgg
     9601 cctgcaaggt accatccgcg ttccggctca tggccgtgac ccgggcgccg cgtatgggct
     9661 gactcgcctg gtccataacg cgcccggtga tcgctccgtg ccgcaccagc cgtatggtga
     9721 ttgccgtttg ctcacgaccg atccgcgccg caattcgcgt tttcatgtat cccggcttct
     9781 cgacggttat caggtactct ccggcgatca accccggcgc tctgaagcgc ccttcgccat
     9841 cggtcacggt gtcgacgact gccgtcccac cgggagacct ctcaatgcgc acctccgcgg
     9901 atgctacggg tgttccggag ttgtcctgaa tgacccggca cgtaagctcc tctgccggtg
     9961 ccgctttcgg catggccgca agcagaagac acaatgccat caccggctgc ttcctgcctt
    10021 cagctttcgg ccgtctcatg tcgattgctc agccgagcga catctcgtac gccgtactga
    10081 ggatgaagcc tggaatccag cccatgacat gcttgtccat gctcatttca cgcttctcgg
    10141 ctgggacggc ccagccggtg taggcctgac ggatgtctcc atcgggagtg aggttcttct
    10201 gaacatattg ccaggcgcga gtcgcgggct ctttgaatga gccgggcagc cagccggatc
    10261 gtactgcctc gtgcaaccct gatgcgaaca tcgcgcttcc ggtcgtttcg agcggtgtgt
    10321 cgggttcatc caacagcacg cggaaaccgc cttcaggatg ctgtacccgg acaatacctt
    10381 cggccagccg gcgcatatct tcgaggaagg ggcgatacgc ggtgtcgctg gcgggaaggt
    10441 ggcgcagcac tccggccatc gcccacaaga gccacccgct tgcgcgcgtc cagtatgtat
    10501 ttcctaaacc ggtcttgttg aagaggatgg ttttggcgag accggtctct ttcaccagga
    10561 acgtatcagt gtaggcgcgg agctgaaaaa cggcttgttc gcgaaacgcg gtttcgttgc
    10621 gcgggtcgag ccgcgaggcg atcatcaacg cgctcgcgac aaagtaacag gtgtcgggaa
    10681 tcgcaaactc ggagtaatcg tcgtgactga caagcccgag atgattgcgg gcggaacggt
    10741 gaaggataat ggacgcaaca tcgacagcaa tgcggcgcgc ccgctcatct tgaagctgac
    10801 gatagatctc ataacaaggc agcgccagcc cgtagtgccc ggcataggtc gtgatgggta
    10861 tgcctccagc gatgaccgtt cgcgacttcg cgccgctata cttgctcacc gacgttaagc
    10921 tgagatgatg atcgagccag cggcgggcat actggcgcac ttccggaaca tgacttccgc
    10981 cccattcgag catgcctttc atcagtaacg taccaaacca atccgtgttc caaacggagg
    11041 gatcctgttc gagagatcga tcgagaatcg ctgcaacggc ggcaggtgtg tcaggtggca
    11101 gcttgcgcgc ggagctggcg ccggcctttg agagagctgc cagggaggcg agagctgccc
    11161 ggcgagttac ggacatggtc atacgtagat tgtcctaacc cgagcactga tgttgcaaag
    11221 aacgctgtag gaaatggttc ccgcaacgtt ggcaatatcc tgggcatcga gactggcgtc
    11281 cccctcgcgg ccaaggatcg tcacgctgtc cccgggcgcc agcggagagc agtctgtcac
    11341 atcgatggtt gtaacatcca ttgagaccgc gcccaggatg gggacgagct tgccggacgc
    11401 gatcagcttg cccttgttcg aaagctgatg cggaacgcca tccgcgtatc cggcggcgac
    11461 caccgcaatc cgggtcggac ggctcgtccg gtacgtgccg ccgtatccga ccagcgaacc
    11521 ttcgggcaga tccttcaccg taagtatgga ggctttccag gtgagtgcgg gtttcacgtc
    11581 gagtatccgc tccggaacgt ctccgcgagg cggtggaata tagccataga tggcatgccc
    11641 ggggcgcacc agttggcccc acgcgcggct gcggccatac gcgacgggaa tggtgctggc
    11701 cagatgcaga tagggcggct taacgcccgc gcggcgcaga tcggagacga tgtcgagaaa
    11761 agttgcggtc tgctcttccg tttgtgatgt gctgtagtcg ccggctgagg caaagtgggt
    11821 catcaggcct tcgagccggc aatgccgtgc gccgttcacc gcatccagga tctccgccgc
    11881 tcccgcccgt gcgcccagac ggcccatccc ggtgtcgatc ttcaggtgat aatgcagggg
    11941 cgttcccgat cggacggcga gctggtccca ctcgcgcagg tccggaaggg aatgaatgac
    12001 tggtgtaagt tcgtattcga tgagaccggc gcgctcggcg ggcaggaagt ccgccatgac
    12061 aagaatacgg gtacggatgc cggcttcccg taaggaagcg ccttcttcca cgctggacac
    12121 cgccagccac tgtgctcctt cgctcaccag aaggcgtgaa acctcaacgg cgccgtggtg
    12181 gtaggcgtcg gctttcacaa cgggcatgac ctcgacggag gctccgacca cattccggac
    12241 ggcccgaaag ttttcggtga tctgacgcct ggatacctcg gtccaactac gaagcgcaaa
    12301 cg
//
LOCUS       DASTMK010000617         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_287682, whole genome shotgun sequence.
ACCESSION   DASTMK010000617 DASTMK010000000
VERSION     DASTMK010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287682"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFQ79_25550"
     CDS             complement(<1..668)
                     /locus_tag="VFQ79_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0143112.1"
                     /translation="MNDYDAVVVGAGPTGLACGIELKQRGVRAVLIDKGCVVNSIYHY
                     PTNMSFFTTPELLEIGGLPMTSIGEKPTRTEALKYYRRVADYYGLDIRQYEMVLKITG
                     GDGNFSVHTEDRNGCVRRYAAKKIILATGYYDRPNLLDVPGEDLPKVIHYYKEAHPYY
                     NHDVAVVGAKNSAAIAALELHWTGARVTLIHRGGGISGSVKYWIKPNIENRIKSGEIK
                     AYFHS"
     gene            complement(670..>2942)
                     /gene="alaS"
                     /locus_tag="VFQ79_25555"
     CDS             complement(670..>2942)
                     /gene="alaS"
                     /locus_tag="VFQ79_25555"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU0143113.1"
                     /translation="YVTVFREDDDAERLWQEVAGVPKDRIFRLDEKDNFWQMGETGPC
                     GPCSEIHYDLGPEAAEPGRADEPFPSDAGGRYVEIWNLVFMQYDRATDGTLTPLPRPS
                     IDTGMGLERIAAVLQGKLSNYDTDLIHPIVLRAAELFHVTFGEDARTDTSLRIAADHG
                     RATTFLIHDGVLPSNDGRGYVLRKIMRRAMRHARMAGVHHPFLYELTGFVAELMKPAY
                     PELMESVQRVARVVKEEEHRYATTFHVAEKIFHDEVKSAAGGVLPGAAAFRLYDTYGL
                     PVDEQEEMAREYGLSIDREGFEREMEQQRERARASWRGAEKAQTAPVYHRLLEHGRTR
                     FIGYDSLESSEAHIIGLVRNREPVETAGVGDEVEVVLDQTPFYAEAGGQVGDQGSLYS
                     AQGDERIAEVKSARAALPGLSVHEVVALQPLRVGQTVRAAVGRELRDATRRNHTATHL
                     LHAALRTVLGNHVKQAGSVVEPGRLRFDFTHYTAMDPEEIREVERLVNQRILENTPVE
                     TNVMELEQALSTGAMALFGEKYGERVRVVSVPGFSRELCGGTHVNRTGDIGLFKIVYE
                     GSISAGVRRIEAITGEGALRRFQYATDSLHRVANLIRVAEPELVEQVERMALHQKALE
                     KQLDQLKSKLAQSQAGGLEAKSREIKGVRVLADRVDGLDRQQMRALADSLRNKWKTAV
                     IVLATAEDSTVAIVSAVTKDLTGKVHAGKLAGAVAQAVGGRGGGRPDMAEAGGKDVAA
                     LPAALDSVYSSVEGML"
BASE COUNT          510 a          961 c          859 g          612 t
ORIGIN      
        1 gaatgaaagt aggccttgat ctcaccgctc ttgatgcggt tctcgatgtt gggtttgatc
       61 cagtacttta cggaacccga gatgccgcct ccccggtgaa tgagtgtgac gcgcgcgccc
      121 gtccagtgca attccagggc ggcgatggcg gccgaatttt tcgctcccac taccgccacg
      181 tcgtggttat agtacggatg agcttctttg tagtaatgga tgactttggg caggtcttcg
      241 ccgggaacgt ccagcagatt cggccggtca taatacccgg tggcgaggat gattttcttt
      301 gccgcatagc gcctcacgca gccattacgg tcttccgtgt gcacggagaa attgccgtcg
      361 ccgccggtga tcttcagcac catttcatac tggcggatat ccaggccgta ataatccgcc
      421 acgcggcgat agtatttcaa cgcctcggta cgcgtaggct tttccccgat ggatgtcatc
      481 ggcaggccgc cgatttcgag cagttccggc gtcgtgaaaa agctcatgtt ggtcggatag
      541 tggtaaatgg aattcaccac gcaccctttg tcgatcagga ccgcgcggac gccccgctgc
      601 ttcaattcga tcccgcaggc aagaccggtg ggtccggccc ccactacaac agcgtcgtag
      661 tcgttcacct cacagcattc cttccaccga cgaataaacg ctgtcgagcg cggccggaag
      721 cgccgctaca tcttttccgc ctgcttcggc catatcgggc cggcctcccc cacgcccccc
      781 tacggcttgc gccacggctc cagccagctt gccggcgtgc accttgccgg tgaggtcctt
      841 cgttacggcc gacacaatgg caacggtcga atcttccgca gttgccagta cgatgaccgc
      901 cgttttccac ttgttgcgaa gcgagtccgc cagcgcgcgc atctgctggc ggtccaggcc
      961 gtccactcta tcggcgagca ccctgacgcc tttgatctcc cgcgacttcg cctccaggcc
     1021 gcccgcctgc gattgggcca gtttgctctt gagctgatcg agctgttttt cgagtgcttt
     1081 ctggtgaagc gccatgcgtt ccacttgctc caccagttcc ggctcggcga cgcgaatcag
     1141 gttggcgacc cgatgcagag agtccgtggc atactggaac cgccgcagcg caccttcgcc
     1201 ggtaatggct tcaatcctcc gcacacccgc ggagatgctg ccttcataga cgattttgaa
     1261 caacccgatg tcgcccgtcc ggtttacgtg cgtgccgccg cacaactcgc ggctgaagcc
     1321 cggcacagag accacgcgaa cccgctcgcc gtatttttct ccgaacagcg ccatagcacc
     1381 cgtggacaaa gcctgctcga gttccataac gtttgtctcg acgggagtgt tttccagaat
     1441 gcgctggttg acgagccgtt ctacttcgcg aatttcttcc ggatccatcg cggtgtagtg
     1501 ggtgaagtcg aaacggagcc gcccgggctc caccacactg cccgcctgct tcacgtggtt
     1561 gcccagcacg gtccggagag cggcgtgcag aagatgtgtt gctgtgtgat tgcgccgtgt
     1621 ggcatcgcgc aattcccgcc caacggcagc gcgtaccgtc tgccccaccc gcagcggttg
     1681 gagtgcgacg acctcgtgaa ccgaaaggcc cggcagcgcc gcgcgtgcgc tcttcacttc
     1741 cgcgatgcgc tcatccccct gcgcactgta gaggctgccc tggtcgccaa cctggccgcc
     1801 cgcttcggcg taaaacggcg tctggtccag caccacttcc acttcatcgc ccacgccggc
     1861 ggtctccacg ggttcgcgat tccggacgag tccgatgatg tgcgcctcgc tcgactccag
     1921 gctgtcgtat ccgataaacc gggtgcggcc gtgttcgagc aaccggtgat aaaccggagc
     1981 ggtctgcgct ttctccgcgc cccgccagct tgcgcgcgcc cgttcgcgct gctgctccat
     2041 ttcgcgttcg aagccctcgc ggtcgatgct cagcccatac tcgcgggcca tctcttcctg
     2101 ctcgtccacg ggcaggccgt aagtgtcata gagccggaag gcggcggcgc ccggcaatac
     2161 accgcccgcg gccgatttca cttcatcgtg gaagatcttt tccgccacgt ggaaggtagt
     2221 cgcgtaacga tgctcctctt ctttcacgac gcgagccacg cgctggacac tttccatcaa
     2281 ctcggggtag gcgggcttca tcagctccgc gacaaacccg gtgagctcat acaggaaggg
     2341 atggtgcacc ccggccatac gtgcgtgacg catggcccgc cgcatgattt tccgcagcac
     2401 ataaccgcgg ccgtcgttcg atggcagaac gccgtcgtgg atgaggaacg tggtggcgcg
     2461 gccgtgatcg gcggcgatgc gcagggacgt atcggtacgc gcgtcctcgc cgaaggtgac
     2521 atggaacaac tccgccgcgc gcagcacgat gggatgaatc aggtccgtat cgtagttcga
     2581 taatttgcct tgcagaacgg ccgcgatgcg ttccaatccc atgccggtat cgatggaagg
     2641 acgcggcagc ggcgtgagcg taccgtccgt ggcgcggtca tactgcatga acacgagatt
     2701 ccagatctcg acgtagcggc cgcccgcatc cgatggaaag ggttcgtccg cccgtcccgg
     2761 ttcggcggct tcgggcccca ggtcgtaatg gatttccgaa caggggccgc agggaccggt
     2821 ctctcccatc tgccagaaat tgtctttttc gtcgaggcgg aagatgcggt ctttcggaac
     2881 gccggcaacc tcctgccaga gtcgctctgc gtcgtcgtcc tcgcgaaaaa cggtgacgta
     2941 ca
//
LOCUS       DASTMK010000618        10859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_575098, whole genome shotgun sequence.
ACCESSION   DASTMK010000618 DASTMK010000000
VERSION     DASTMK010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575098"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..833)
                     /locus_tag="VFQ79_25560"
     CDS             complement(111..833)
                     /locus_tag="VFQ79_25560"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143114.1"
                     /translation="MDYLLRDAHHAGVAYGKFDQLRLIGTLRILPREDQETNEPDMGI
                     TGGGLQAAESLLWGRYFMYTQLYFHPVRRIYDMHLTEFLTQALPGGKYEIDLGQHLAT
                     TDNEILSEISRASVDPTARAHVTAARIAERRHFRLLYESTPLDLQRNLKSPELIYTAA
                     RKEFCDDLVRFNPYKSNADSEDFPVFQRDGSITSSVLLSTTFAQPPRFAVAYVFVAPE
                     IKEKAKAWLRSAKDKILSGDGR"
     gene            complement(960..1121)
                     /locus_tag="VFQ79_25565"
                     /pseudo
     CDS             complement(960..1121)
                     /locus_tag="VFQ79_25565"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(1508..2788)
                     /locus_tag="VFQ79_25570"
     CDS             complement(1508..2788)
                     /locus_tag="VFQ79_25570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143115.1"
                     /translation="MTLSRREFLGAAGAAAIPSLDGASVRPASARVPMPFFGLHRFIE
                     ENPKAVFIRRTHVPHKMDAPAKLREGLNLAREIFVPMDRAGVPVTHSVVLKPNFTSVR
                     GRRRTPEENWGTGTDPQFYEGMVMGLKEIGLKKFHFIEANNYPMWNHRGFLDINERHG
                     IAMNETDRRPRHFRDGDGITWSKVPDSVIYSRVPHYAPVNEPGTWLLNISKWKAHGMC
                     LTQAVKNEQGLVVSPFVRFCSGWQGVTGVPEVMKPEINPRAETLIKNFFERHKKMGYA
                     RYDSAARLGPIHQEIWAHKTCDNQSVLRTGLSMIEGIYSRDGDGFGQGDDYLTNLVLF
                     GIDKFRLDVIGLYLGGHEPGNVALYRIAKERGLSDTFNPWEIPVFEWVDGAAVPRKLT
                     DFPRTPLKTYYLQKENEPIFHLVDEPFDYDRNKI"
     gene            complement(2817..4106)
                     /locus_tag="VFQ79_25575"
     CDS             complement(2817..4106)
                     /locus_tag="VFQ79_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143116.1"
                     /translation="MAIDLARDIVLRWEDQDPALAPMLKDGGTDVVLVDKPVDAFLQA
                     CQKAGLRVASSDEIVLSGLQEFRNAGGRPAALTEGLWPGISRTPNTTGAEDVASASHQ
                     PWVDANGFRIAWLKALCPERQPAVAYKPDEHSGVSPDRVLPYDSLELALVEAWVSGAN
                     YVLAVEPRYRKALVDGDAKAIEAWRRMGRSARWLKEHKPLFGHPFFPAITMLVDEDEL
                     SAELANLAFRQNACPALEPAARPPAPDPDKRPVVVAASLTAPEPGVRSRILAHAEAGS
                     TLIVDREDEKPWWHVPALKLEREEPDRETYRLGRGKVIAYRELISDPSDFALDMIDAA
                     THQRRAVRIWNALSVIATATSGPASGPFRARAIVQAINYGRSLRYEILMRVSGEYSSA
                     VLLRPEGERVKLTTARRGTGTEFTIPHLEQVGIVLFS"
     gene            4311..5102
                     /locus_tag="VFQ79_25580"
     CDS             4311..5102
                     /locus_tag="VFQ79_25580"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU0143117.1"
                     /translation="MTAQRFGSLRVYVHPDRVSMGGAAAAVAASGIKRLIDENGVANV
                     LFSSAVSQLDCLDALASDNSIDWNRVVAFHVDEYAGMPITHPASFRRFLLDKFLFKVA
                     PREFHGIAGESPDPAAECARYASLLNEHPPGLTILGIGENGHLAFNDPPVDFKDPFDV
                     KPVTLDEKCRRQQVNDGAFASIEEVPPVALTVTISRLMRVPRIVCVVPGVRKSEAVKR
                     TLLEAVSPDCPSSILRTHPNAELHLDAEAASLLDVEHLMETGARR"
     gene            5172..6542
                     /locus_tag="VFQ79_25585"
     CDS             5172..6542
                     /locus_tag="VFQ79_25585"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEU0143118.1"
                     /translation="MLSRRDLFQFAAPALAGSAAGFQGPSAKDRVVLVISVDGFPGWE
                     WQNPRLPVPNLWKLAQSGVRAETVKITNPTVTWPTHTSMITGVRSARHGVLANGLVQR
                     NSDSKPVTVKPWHDREDLVQATTIYDLAYDAGLTTAEVDWVAIYNAKRITWRFPEVPD
                     PAGKIEREMIEAGLIFELDIKEFFKSSPVWRDQQWTQAGVHIITKHAPNLLLFHLLNT
                     DAANHAYGPRTPASQTAYAFADECVAKLLNALKTANLWERATVFIVTDHGFKPITSAV
                     KPNAILRQMGLVRGSGSAIECDAWVLPIAGIAGVYFVNPEKKAGLAARLPEVFAKAEG
                     VLRVLAPDEFAAFDLPHPDRNSRAPDLVLVAKDGYTFNAAHEGDATGPAPYRGTHGYP
                     ASDPDMNGTFIASGYGIRKGARLDQMSHLDVGPTIGALLGIEMKNVEGRVLTEILDAS
                     ALSRRM"
     gene            complement(6548..9784)
                     /locus_tag="VFQ79_25590"
     CDS             complement(6548..9784)
                     /locus_tag="VFQ79_25590"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0143119.1"
                     /translation="MGKRLGFLLLLCAAIVTAQTQGTISGYVRDASGAIVPGASITIT
                     HEGTGASRNTLSDAAGFYQVLGLVSGTYTIEAEVSGFKKYRNAGVVLRVDENVRADIS
                     LDVGQVSESVEVSAQAALVDTRSSQTSATIDDRRIVDLPLRGRNVFALAATLPGVLNV
                     RAPDNSDLGDTRAGPTMNVNGGRANMNYNRFNGTYFNNPSRNTGLNVPPPDAVQEFKI
                     QTSNFAADSGRNPGANITVVSRQGTNEFHGAAWEFHRNDNLNARSFFQTAKPQLIQNQ
                     FGAAAGGPIMRNRMFVFGTYEGLRDRRQAATTTALPPTRAEASGDFSHLNGVKQLVNP
                     ADGTPFPGNRIPASLLDPAALRVLDFVPVVDSSGIIQAVGPNPRNSDLAMLRWDLILT
                     QKQSLFAHYYFNQTRQENPELAYGSNIARWTGQKQGPRFQNAGINHTWAVSPSLLNQL
                     TLGFTRSFSLNTPTVTRTPADLGIAGMPMYTDGGSPQFNVSGRFSLNSGGPVKFVSNV
                     YQLQENVSLVRNRHTFRFGFEHMRLSFFQSFLGPPQFTFNGQRTGGGTATLGDPLADF
                     LLGTYQELPVTNGVRVNDGSNTFTAAYIQDDFKVSSRLTLNLGLRYELPTPWADKYDR
                     INTVIPDANIRSGKFPDAPPGMLFPGDLPRGLYDTDKNNFAPRFGFAWDIFGDGRTAV
                     RGAYGIFYDTFNTDTVAQENPPFVGGRRTFRGGYVANPFGSVGESAPPAFIDPAAFTF
                     VFPINGFWSGTGKDSLRTTYVQEWNLTIARDLGRDYAVSAAYIGKTGRKLIAYRPFNA
                     APYIPGVDSQGRPLSTEANAESRAPFLPGIYGTEGIYLDNSFTSSFHSMQLEVNKRFA
                     HGLQFSTSYTLGKSIDSSSTTTLGGCLSNPFDVRSDRGRSDWDRRHGFVFSGVWSPAV
                     MTDQRGFLGRIFGGWTASGFQTVQSGSPVTPVSGQNTALDGNICGGSSIHPDLVGQIE
                     RNHSSRADMILNFFNRDAFALPAIGRYGSAGRGIFSGPAMVSTDLAILKDFPVTEQHR
                     LQFRAEFFNLFNQVNFNNPVANLSSATYGRITGSQPGRAIQFGLKYLW"
     gene            9898..>10859
                     /locus_tag="VFQ79_25595"
     CDS             9898..>10859
                     /locus_tag="VFQ79_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU0143120.1"
                     /translation="MLLSRVVETSRKVAETSKRLEKLDLLATLLRQLTPDEAEIVIPF
                     LSGATRQGRIGIGHATLRDAASPPVENGSLEIFEVARVFEEITRVGGRGAERQKQQLL
                     HSLLSRSTQAEQQFLTALLMGELRQGALQALMVEAVAKAAGLAPERVRRAVMVAGDVV
                     PVAQAVLSSGEAGLARFSIELFRPVQPMLAQTAEDMDQAIACLGEAALEFKLDGARIQ
                     VHKSGSDVVVFSRRLNDVTVAVPEVVEAVRALPAQDVILDGEVLSLQPGGRPQPFQIT
                     MRRFGRKLDVERMRAELPLQPFWFDLLHINGADVTEEPQTRRFAA"
BASE COUNT         2146 a         3243 c         3247 g         2223 t
ORIGIN      
        1 aacgtatgtc gccttctgga gatgggtctc accagcccaa ctaccatggt cctttagttt
       61 ttccgtgagg gagagcaaga tgcttgccga ctctaaccta ttcattttaa ctaccttcca
      121 tctccggaca gaatcttgtc cttagctgat ctcagccacg ccttcgcctt ttctttgatc
      181 tccggggcaa cgaatacata ggctaccgcg aaccgagggg gctgcgcaaa agtcgtcgaa
      241 agcagaacgg aagatgtgat gctcccgtcc cgctggaaca ccgggaagtc ctcggaatcc
      301 gcgttcgact tgtacgggtt gaaccgaacc agatcgtcac agaactcctt cctggccgct
      361 gtgtagatga gttctgggct cttcaggttt ctttgcaagt ctagtggcgt actttcgtag
      421 agtaaccgga agtgccgacg ttcggcgatc ctggcagccg taacatgtgc acgcgccgtt
      481 ggatcgaccg aagccctgga gatctcagat aagatctcgt tgtcggtcgt cgcaagatgc
      541 tgtcccaagt caatctcgta tttgcctcct ggaagtgcct gagtcaggaa ctccgtaaga
      601 tgcatatcgt agatgcggcg tacagggtga aagtacaact gtgtgtacat gaagtaccgc
      661 ccccaaagga gcgattcggc tgcctgtaat cctccgccag ttatgcccat atcgggttca
      721 ttggtttcct gatcctcgcg aggcaaaatg cgtaaggtcc cgatcaagcg gagttgatcg
      781 aacttcccgt acgccacacc tgcgtgatgg gcgtcacgca gcaagtagtc cattcgatcc
      841 gcaccaaaaa cgtcgcctcc gatgatctcg tacagaatcg actcccaagt cgtaaactga
      901 taggtttcct tacctcgggt ccgcgcgaag tacttgggac caacagcgat tttggcgacg
      961 tcctcagcgc tgatcttcag atctcgaaac gctgcagcca gaaactcgga gaagatcaaa
     1021 tcgcgactca gatcttcgtg gcgaatcccg tccggcaaca ggtccttctc cgcggcatgt
     1081 gagaaaggaa ggtggcccaa gtcgtggcac agggcggccg cccgaacgac gcggcgccag
     1141 taggtctatt cgtgctctcc gaggcatgga acaagatcgc gaacgttatc atgcttgatg
     1201 ttttccggat ctgttaccac gtcatagatc cgccccgcca aatccatcac accaagtgag
     1261 tgttcaaacc ttctatgcgt ggccgcggga tacacaaggt aggtcagcgc aagctgatgg
     1321 atgttccgca gacgctggaa cggtctcgag ttgacgagct ttcgttcctc cgaatccagc
     1381 tttatgaatc cgtggattgg gtcccggatc tcgtggattc aattggacat gccatagtga
     1441 ttgtacttcc ctggtcaaaa catttccaga ggggattgtt caatacgttc aatgcacggc
     1501 tttccgctca gatcttgttc cggtcgtaat cgaacggctc gtccaccaga tggaagatgg
     1561 gttcgttttc tttctggaga tagtaggtct tcaatggtgt tcgcgggaag tccgtcagtt
     1621 ttcgcgggac cgccgcgcca tcgacccact caaatactgg aatctcccat gggttgaacg
     1681 tgtccgacag gccgcgttcc ttggcaatac ggtatagtgc gacgttgccg ggctcgtgcc
     1741 ctccgagata gagcccgatc acatcgagcc ggaacttgtc tattccaaaa agcaccaggt
     1801 tcgtcagata atcgtcgccc tggccgaagc cgtctccgtc ccgcgagtag atcccttcaa
     1861 tcatcgacag gccggtgcgt aaaacactct ggttatcgca tgttttatgc gcccagatct
     1921 cctggtgaat ggggccgagc cgcgcggcgc tatcgtagcg cgcgtagccc atcttcttgt
     1981 gccgttcgaa gaagttctta ataagcgtct cggcccgcgg attgatttcc ggcttcatca
     2041 cctccggaac gccggtgacc ccctgccacc cggagcagaa tctcacaaac ggagaaacga
     2101 ccaggccctg ttcgttcttg accgcttgcg tgaggcacat tccgtgggct ttccacttgg
     2161 atatattcaa cagccacgtg ccgggttcgt ttacgggggc gtagtgcggt acacggctgt
     2221 aaatcacgga atccggcacc ttgctccaag tgatgccgtc gccgtcccgg aagtgacgtg
     2281 gacgccggtc cgtttcgttc atggcgatgc cgtgccgctc gttgatatcc aagaaaccgc
     2341 gatggttcca catcgggtag ttgttcgcct cgatgaagtg gaactttttg agcccgattt
     2401 ccttcaagcc catcaccatt ccttcataga actgcggatc cgttcccgtg ccccagttct
     2461 cttcaggcgt ccgccgccgg ccgcggacgc tggtgaaatt cggcttcaaa acaacgctgt
     2521 gggtgacggg cacgcccgca cggtccatcg gaacgaagat ctcgcgcgcg agattcaagc
     2581 cctcgcggag tttggcgggg gcgtccatct tatgcggaac atgagtccgc cggatgaaaa
     2641 cagctttcgg gttctcttca ataaagcggt gcaacccaaa aaatggcatg ggcacgcgcg
     2701 cacttgcagg ccgcacggac gcgccatcca aggaagggat tgcggcagcg cctgccgccc
     2761 ccaggaattc acgtcgcgag agagtcatcg taaatagctc cctgtcaaaa acagctctaa
     2821 ctgaacagta cgattcccac ctgttccaga tgaggaatag tgaattcggt tccggtgccg
     2881 cgccgggcgg tcgtcagttt tacgcgctct ccctcaggcc gcaggagaac ggcagaggag
     2941 tactctccgg aaactcgcat cagaatctcg taccgcaagc tccgcccata gttgatggcc
     3001 tgcacaatgg cgcgcgcgcg gaacggtccc gacgccgggc ctgatgtcgc ggtggcgatc
     3061 acgctcaacg cgttccatat tctgacggcc cgccgctgat gcgtagcggc gtcgatcatg
     3121 tcgagggcaa aatcactcgg gtcggagatg agctcccggt aggcgatcac cttcccgcgg
     3181 cccagccggt acgtttcgcg gtccggctcc tcacgctcca gtttcaaagc tggaacgtgc
     3241 caccagggct tttcgtcctc ccggtcgacg atcagcgtgg aacccgcttc cgcgtgagcg
     3301 agaatccggc tgcgtacgcc gggctccggc gcagtcaggc tggcggcaac cactacgggc
     3361 cgcttgtctg gatcgggcgc cggtgggcgg gcagcgggtt cgagcgccgg acaggcattc
     3421 tggcggaagg caaggttcgc gagctcggcg ctgagttcgt cttcatccac cagcatggtg
     3481 atagcgggaa agaacggatg cccgaaaaga ggcttatgtt ctttcagcca gcgcgcggag
     3541 cggcccatgc ggcgccatgc ctcaatcgcc tttgcgtcgc catccacaag cgccttgcgg
     3601 taccgcggct ccaccgccag gacatagttg gctccgctca cccacgcctc aaccaatgcg
     3661 agttcgagcg agtcgtaggg gagaacccgg tcgggagaga ctccggagtg ctcgtccggc
     3721 ttgtaggcta ctgccggctg ccgttccggg caaagcgcct tcagccatgc gatacgaaat
     3781 ccattcgcat ccacccacgg ctgatggctt gcactcgcga catcttcggc accggttgta
     3841 ttcggcgtgc ggcttattcc aggccatagg ccttccgtca gagccgccgg acgtccgccc
     3901 gcatttcgaa actcctgcaa gccggacaga acaatttcat cggacgaggc tacccgcagc
     3961 cccgcctttt ggcacgcttg gagaaacgca tcgacgggtt tgtcgaccag taccacatcc
     4021 gtccccccat cctttaacat cggcgccagg gcggggtcct gatcttccca tcgcaggaca
     4081 atgtcgcgcg cgagatcgat ggccataaat ctcctgctcg aagtctacga cattgacgat
     4141 ttcaatacaa gatggtctga aaggcgaaaa ttgtcaagtt gcggccacgc agacccaggt
     4201 gcaaggaatt gcgtccataa atatggatat ggacgggagt ggagttggaa aaatacggct
     4261 taaattgaga caattaagta tggaaatgaa tattagggga ttcacataac atgactgctc
     4321 aacgatttgg ctcgcttcgc gtctacgttc acccggaccg ggtatcgatg ggtggcgccg
     4381 ctgcggcggt ggcagcctcc gggatcaaaa ggctcatcga tgaaaacggc gtcgcgaatg
     4441 tacttttctc ctctgctgtc tcccagctcg actgcctcga cgcactcgcc tccgacaatt
     4501 ccatcgactg gaatcgtgtc gtcgcttttc acgtcgacga gtatgcgggg atgcccatca
     4561 cacatcctgc gtccttccgc cgcttcctgt tggataaatt ccttttcaag gtggccccgc
     4621 gcgaattcca cggcattgcc ggcgaaagcc cggatcccgc cgccgagtgc gcgcgctatg
     4681 cttcgttgct gaacgagcat ccccctggtc ttacgattct cggcatcgga gagaacgggc
     4741 atctcgcctt caacgatccg ccggtggatt tcaaagatcc gttcgatgtg aagccggtga
     4801 cgctcgatga gaaatgccgc cggcagcagg tcaacgatgg ggcgtttgcc tcgatcgaag
     4861 aagttccacc cgtggcgctg acggtgacca tctcgcggct catgcgcgtt ccgcgcatcg
     4921 tgtgtgtggt gcccggcgtt cgcaagagtg aggcggtgaa acgcactctc ctggaagccg
     4981 tgtcgccgga ctgcccctct tccatcctgc gcacgcaccc caacgcggag ttgcatctgg
     5041 atgccgaagc cgccagcctg ctggatgttg aacacctgat ggaaaccggc gcccggcgct
     5101 gatccgcttt tcgcctgtag ccgcagcgcc ggcagaccgg tccggttcat cgtaaactgg
     5161 atgcgatcat tatgttgtcc cgccgcgatt tattccaatt cgcagcgccg gcgctggcag
     5221 gctccgccgc tggttttcaa gggccttccg ccaaagaccg cgtggtgctg gtgatcagcg
     5281 tcgacggctt tccgggatgg gagtggcaga acccgcgcct tcccgttccg aatctctgga
     5341 agcttgcgca gtcgggtgtg cgcgcggaga ccgtgaagat cacgaacccg acggtcacct
     5401 ggcccaccca cacatccatg attaccggcg tgcgctccgc acggcacggt gttttggcga
     5461 atggactggt ccaaagaaac agcgacagca agcccgttac cgtgaagccg tggcacgaca
     5521 gggaggacct tgtacaggcg actacgatat acgatctcgc ctacgacgcg ggacttacta
     5581 ccgccgaggt ggactgggtc gccatctaca acgcgaaacg gattacgtgg cgttttcccg
     5641 aggtgccgga tcccgccggg aagatcgagc gcgagatgat cgaggccggg ctcatcttcg
     5701 agttggatat caaggagttc ttcaagagca gtcccgtctg gcgcgatcag cagtggacgc
     5761 aggccggagt gcacatcatt accaagcacg ctccgaacct gctgctgttt catcttttga
     5821 atacggatgc ggccaatcat gcctacggcc cgcgcactcc cgcatcgcaa accgcttacg
     5881 catttgccga tgagtgcgtg gcaaagctat tgaacgcact caagaccgcg aacctttggg
     5941 agcgggcgac ggtcttcatc gtcaccgatc acgggttcaa gcccatcacg agcgcggtga
     6001 agccgaacgc aatcttacgc cagatgggcc tggtccgcgg ttccggctcc gcgatcgaat
     6061 gcgatgcctg ggtgcttccc atcgccggca tcgccggcgt gtatttcgta aatccggaga
     6121 agaaggccgg tctggccgca cgcctgcccg aggttttcgc gaaagcggaa ggtgtcctgc
     6181 gcgtgctggc gcccgatgaa tttgcggctt tcgacctgcc gcatccggac cggaacagtc
     6241 gcgcccccga cctcgttctg gttgcgaaag acggatacac gttcaacgcg gcgcacgagg
     6301 gcgatgcaac gggaccggcg ccttatcgcg gcactcacgg ctatccggcg tccgatcccg
     6361 atatgaacgg tacattcata gcatccggct acggcattcg aaaaggcgcc aggctcgacc
     6421 agatgtccca cctcgatgtc ggccccacca tcggtgcgct actgggcatc gaaatgaaaa
     6481 acgtcgaagg ccgcgtgctg acagaaatcc tggacgcctc cgcgctgtcg cgccggatgt
     6541 gagtcagtta ccagagatac ttcaacccga actgaatggc tcgtcccggc tgcgagcccg
     6601 tgatccggcc ataggtcgcc gaggatagat tcgccaccgg attgttgaaa ttcacctggt
     6661 tgaacaggtt gaaaaactcc gcacgaaatt gcagccggtg ctgctcggtc acggggaagt
     6721 ccttgagaat cgccaggtcg gtcgagacca ttgcgggacc tgagaaaatg cccctgcccg
     6781 ccgagccata gcggccgatg gccggcaacg cgaacgcgtc gcggttgaaa aagttcagga
     6841 tcatatcggc cctggaactg tggttgcgct cgatttgccc caccaggtcc gggtggatgg
     6901 aactgccgcc gcagatgttg ccatcgagag ctgtattctg gcccgaaacc ggtgtcaccg
     6961 gactgccgct ctggactgtc tggaatcccg aggctgtcca tccgccgaat atccgcccca
     7021 gaaaacctct ttggtccgtc atcaccgccg gcgaccagac gccggagaag acaaacccgt
     7081 gccgccggtc ccaatcggac cgcccgcggt cggagcgcac gtcgaacgga ttggaaaggc
     7141 aaccgccgag tgttgtcgtc gaactgctgt cgatcgattt gccgagcgtg tacgatgtcg
     7201 aaaactgcaa cccatgcgcg aagcgtttat tcacttccaa ttgcatgctg tgaaatgaac
     7261 tggtgaagct gttgtcgagg tagatcccct cggtgccata gattccgggc aggaatggcg
     7321 cgcggctttc ggcgttcgct tccgtggaga ggggccgccc ctgggagtcc acgccgggta
     7381 tatagggcgc ggcgttgaac ggacgatagg cgatcagctt gcgccccgtc ttgccaatat
     7441 aggcggccga gaccgcatag tcccggccga ggtcgcgcgc gatggtcagg ttccactcct
     7501 gcacgtatgt ggtgcgcagg ctgtccttac cggtgccgct ccagaaaccg ttgatcggga
     7561 atacgaacgt gaatgccgcg ggatcgatga acgcaggagg agcactctcg ccgaccgacc
     7621 cgaacgggtt ggctacatac ccgccccgga acgttcggcg gccgcccacg aacggcggat
     7681 tctcctgcgc gacggtatcc gtgttgaagg tgtcgtagaa gataccataa gcaccgcgta
     7741 cggcggtgcg gccgtcgccg aaaatgtccc aggcgaagcc gaagcgcggc gcaaaattgt
     7801 tcttgtccgt atcgtacaag ccgcgcggca ggtcgcccgg gaacagcatg cctggaggcg
     7861 cgtccgggaa cttgcctgag cgaatgttcg cgtccgggat cacagtatta atgcgatcgt
     7921 acttatccgc ccagggcgtg ggtaattcgt accggagccc gaggttcaat gtcagccggc
     7981 tggagacttt gaaatcgtcc tgaatgtagg ccgccgtaaa ggtattgctg ccgtcgttga
     8041 ctcgcactcc attggtaacc ggcagttcct ggtaggtgcc gaggaggaag tccgccaggg
     8101 gatcgccgag agtggcggtg ccaccgccgg ttcgctgccc attgaaggta aactgaggcg
     8161 gcccgaggaa cgattggaag aagctgagcc gcatgtgttc gaagccgaac cggaaggtgt
     8221 ggcggttccg taccaggctg acattttcct ggagctggta cacgttggag acgaatttca
     8281 ccggcccgcc ggagttcagg ctgaaccggc cgcttacgtt gaactgcggc gaaccgccgt
     8341 ccgtgtacat cggcattccc gcgattccca agtctgcggg agtgcgggtg acggtgggag
     8401 tgttcaacga aaaggaccgc gtgaagccca aggtcagttg attcagcaat gacggtgaaa
     8461 cggcccacgt atggttgata cccgcgttct gaaacctggg tccttgcttc tgtccggtcc
     8521 aacgggcgat gttgctgcca tatgcgagct ccgggttctc ctgccgggtc tggttgaagt
     8581 agtagtgcgc aaacagagac tgcttttgcg tcaggatcag atcccaacgc agcatggcaa
     8641 gatcggaatt gcggggattg ggtccaactg cttgaatgat gccgctcgag tccacaacgg
     8701 gaacgaaatc gagcaccctg agcgcggcgg gatcaagcag cgaagccgga atacgattgc
     8761 ccggaaaagg cgtcccatcc gccggattca ccaactgctt cacgccattg agatgcgaga
     8821 aatcgccgga cgcttcggca cgggtgggcg gcagcgcggt tgtggtcgcc gcctgcctgc
     8881 gatcgcgcaa tccctcgtac gtcccgaaga cgaacatccg gttgcgcatg atggggcctc
     8941 cggccgccgc accgaactgg ttctgaatca gttgcggttt ggcggtctgg aagaagctgc
     9001 gggcgttcag gttgtcgttg cggtggaatt cccaagccgc gccgtggaat tcgttggtgc
     9061 cctggcggct taccactgta atgttcgcgc cggggttgcg gccgctgtcc gccgcgaaat
     9121 tgctggtctg gatcttgaac tcctgcacgg catccggcgg gggcacgttc aggcccgtgt
     9181 tccgcgacgg attattgaag taagtgccgt tgaaacggtt gtagttcatg ttcgcgcgcc
     9241 cgccgttgac gttcatcgtc ggacccgcgc gggtatcgcc caggtcgcta ttgtccggtg
     9301 cgcgcacgtt caggacgccc ggcagcgtgg cggccagtgc gaacacattg cgcccgcgca
     9361 gcggcagatc tacaatccgc cggtcgtcga tggtggcgga ggtctgcgaa gacctcgtgt
     9421 ctaccagtgc cgcctgcgcc gacacctcga cgctctccga aacctgtccg acgtccagcg
     9481 agatatcggc ccggacgttt tcgtccacgc ggagcaccac ccctgcattg cgatatttct
     9541 taaatccgga gacttcagcc tcgatcgtat aagtgccgga aacgagtcca agtacctgat
     9601 agaatcccgc cgcgtcgctc aacgtattgc gcgatgcacc ggtgccttcg tgtgtaatgg
     9661 tgatggatgc accgggcacg atcgcgccgc tcgcatcccg cacatatcca ctgatcgttc
     9721 cctgcgtctg cgctgtcacg atggccgcgc aaagcagtag caggaacccc agtcgtttgc
     9781 ccatgaattt tccccctgcg aagcatcata tcaattccga tgcttccgct tgttggcgcg
     9841 cgcagctcgg tttcggagcc gtgagcgaac ttctgaagcg tgtactaagc ttgcatcatg
     9901 ctgctgtcca gggtggtgga gacctcgcga aaagtggcgg agacgtcgaa gcgcctggag
     9961 aaactcgacc tgttggcaac gctcctccgg caattgactc cggacgaagc cgaaatcgtc
    10021 attccatttc tttcgggcgc tacccggcag ggacgcatcg gcatcggaca cgcaacgctc
    10081 cgcgatgcgg ccagcccgcc ggtggaaaac ggctctttgg agatcttcga ggtggcccgg
    10141 gtcttcgaag agattacgcg tgtcgggggc cgcggcgccg agcggcaaaa gcagcagctt
    10201 ctccattccc ttctctcccg ctccacgcag gcggagcagc agtttctgac cgcgttgctg
    10261 atgggcgagc tgcggcaggg ggcgcttcag gcgctaatgg tggaagccgt cgcaaaagcc
    10321 gccgggctcg cgccggagcg tgtgcggcgc gcagtcatgg tggccggcga cgttgtgccg
    10381 gtagcacaag cggtgttgtc ctcgggcgaa gccgggctcg cccgcttctc catcgaactg
    10441 ttccggccgg tgcaaccgat gctggcgcag accgccgaag acatggatca ggcgatcgcc
    10501 tgtttgggag aagccgctct cgagttcaaa ctcgatggcg cgcgcattca ggtccacaag
    10561 tcgggaagcg acgtggtggt gttctcccgg cgcctcaacg atgtgacggt ggcggttccg
    10621 gaagtggtgg aagcggtgcg ggcgctgcct gcccaggacg tcatcctgga cggcgaagtg
    10681 ctcagccttc aacccggcgg acgtccacag ccttttcaaa tcaccatgcg gcggttcggg
    10741 cgcaaactgg atgttgagag aatgcgcgcg gagcttccgc tccagccgtt ctggttcgac
    10801 ctactgcata tcaatggagc cgacgtaacg gaagagccgc aaacgcgccg gtttgcggc
//
LOCUS       DASTMK010000619         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_575208, whole genome shotgun sequence.
ACCESSION   DASTMK010000619 DASTMK010000000
VERSION     DASTMK010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575208"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1545)
                     /locus_tag="VFQ79_25600"
     CDS             complement(310..1545)
                     /locus_tag="VFQ79_25600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143121.1"
                     /translation="MTGFRPSRRSMLASLLGAAPFASGTERLPPVRAITRGPRHHWFG
                     YYDKLEFDPGSRYVLGMQVDFEHRSPRPDDVIGIGMVDIQSGDRWIELGRSRAWGWQQ
                     GCMLQWIPGSDSEIIWNDRQGDRFVAYILNVKTRKRRTLPAPVYALSPNGKWAISTDF
                     SRLNDCRPGYGYAGIADPGKDTAAPVDNGIWCIDLASGQRDLLISYAAAARIPYEKGG
                     LERAKHWFNHLLVSPDGGRFIFLHRWRGPEHGRSWETRMFTAKADGTDLYVLDPYGKT
                     SHFIWRDPRHVLAWAWHPSHGDKFYLYRDRSRQVEAVAPDVMTGNGHCTYLPGNQWIL
                     NDTYPDKNRNQNPYLYKVSTGRRYPLGHFHSPPEYQGEWRCDTHPRFSPDGKQVVIDS
                     PHGGNGRQLYLIDISGITG"
     gene            1591..2718
                     /locus_tag="VFQ79_25605"
     CDS             1591..2718
                     /locus_tag="VFQ79_25605"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0143122.1"
                     /translation="MHRETGWFSRERVLVLTLLAATVLALYLCYLIAVPFLPSLVWGL
                     AFAVVADPLRCRIERRIQNRNVAAALTVVLVAVVIMGPVALIGQQVFQQVASGVQSLD
                     SGLPSGGWRETVLRNPALGPVFQWVESYVDIEARLKDAGAAVAERMSSWVTGSVWAMA
                     QFFITFFLLFYFLRDRRAVLNALRSLVPLSRAETDEVFQGVEDSIHATIYGTLTVALM
                     QGALGGLMFWWLGLPAPLVWGVVMAGLAIIPILGAFIVWVPAALFLLAGGYWVKALIL
                     SLWGAVAIGLIDNILYPVLVGSRLRLHTVPVFIAVVGGVIVFGTSGIIVGPIVLAVTM
                     ALVDIWKRRTAAYSTAEEAIGKNLATGLTEPEAKPANPVTL"
     gene            2727..>3642
                     /locus_tag="VFQ79_25610"
     CDS             2727..>3642
                     /locus_tag="VFQ79_25610"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU0143123.1"
                     /translation="MRPNPPESPFQKRRTSVAAAFSARGLDGLLVTALPNIRYLTNFT
                     GSNGALLLFREHAILYTDPRYELQASEEADCPVKVARGPLWAAVRKDLSRKRLKRTGF
                     EKGRITYEAYEALRGSDAGDSGLVPLGGIVEYFRMVKSEDEVEAIRRSVATASKAFHE
                     AVKRIRPEMTEKELAAELDYRMRRLGAEKPAFETIVASGMRTAFPHANPASGTIGRNR
                     LVLIDMGASQNGYASDMTRMLFSGRPGAKLQRLYNSVLEAQLAAIAAVHEGVTAGQVD
                     GKARQVLGRYGLRDAFVHSTGHGLGLEIH"
BASE COUNT          683 a         1093 c         1081 g          785 t
ORIGIN      
        1 cctcctttgt aaatgggtgc ggaacacttc cccagcaatg cgtacagata gcgccgatca
       61 tctcgtggat tgccggggtg tgtcaataga gcatctgtaa atttattgcc atgaatgtga
      121 cgtctcgtgc ggttgattgc cccattgtct caggctgaga aatctcctcc atcagatcca
      181 cctccgtttg gattcacatc aagaaagcga attcccagaa tgttcctgag acattctagg
      241 agagaaagcg aacaatgcgc aagagttcac gcgtgcaaag cagacgaatt tacaacctgg
      301 cggaggcggt catcccgtaa tgccgctgat gtcgatgaga tacaactggc ggccatttcc
      361 gccgtgaggc gagtcgatca ccacttgctt cccgtccggg ctgaatcgcg ggtgcgtgtc
      421 acagcgccac tcgccctggt attccggcgg cgaatgaaaa tgccccagcg gatagcgcct
      481 gccggtggac accttataaa gatacggatt ctgattccgg tttttgtccg ggtaggtatc
      541 gttgagaatc cactggttcc caggcaggta ggtgcagtgg ccattcccgg tcatcacatc
      601 cggcgcgacc gcttccacct gccggctcct gtcccgatac aggtagaact tgtccccgtg
      661 agatggatgc caggcccatg cgagcacatg ccgcgggtcg cgccagataa aatgcgacgt
      721 ctttccatag ggatccagga cgtaaagatc cgttccgtcg gccttggcgg tgaacatgcg
      781 ggtctcccag cttcggccgt gctcgggtcc tcgccagcgg tgcaggaaaa tgaaccggcc
      841 cccatcggga gagaccaaaa ggtggttgaa ccaatgcttg gcgcgttcga gcccgccctt
      901 ctcgtaagga atccgtgccg cggccgcata ggagatcaac aggtcgcgtt ggcccgaggc
      961 gagatcgatg caccagatgc cattgtcgac cggcgcggcg gtatcctttc caggatccgc
     1021 tatgcccgca taaccgtagc cggggcggca atcgttcaga cggctgaaat cggtcgagat
     1081 cgcccacttg ccattcgggc tcagggcgta gaccggcgcc ggcagcgtcc ttcgtttccg
     1141 cgtctttaca tttaatatgt acgcgacgaa gcggtctccc tgccggtcgt tccaaatgat
     1201 ttcggaatcc gaaccgggaa tccattgcag catgcagccc tgctgccacc cccacgcgcg
     1261 gctccttcct aattcgatcc agcgatcgcc ggactgaatg tccaccatgc cgatccctat
     1321 aacgtcgtcc ggccgcggcg aacgatgttc gaaatccacc tgcattccga gcacatagcg
     1381 gctacccgga tcgaactcca gcttgtcgta ataaccgaac caatggtgtc gcgggccgcg
     1441 agtgatggcc cggacgggcg ggaggcgctc cgtgccggaa gcgaaaggtg cggcgccgag
     1501 cagcgatgcc agcatgctgc gccgcgatgg tctgaatccg gtcaacgtac ccaccttcga
     1561 atggtaactt gaaaggagga aggggttcgg gtgcatcgcg aaaccggttg gttttcacgg
     1621 gaacgtgtgc tggtccttac cttgctggcc gcaactgtgc tggcgctcta cctctgctat
     1681 ctgatagccg ttccatttct gccatcgctg gtttggggcc tcgccttcgc cgtggtcgcg
     1741 gacccgctcc gttgccggat cgaacgccgc attcagaacc ggaacgtggc ggcggcgctc
     1801 accgttgttc tggtagccgt tgtcatcatg ggccccgtcg ctctcatcgg gcagcaggtc
     1861 ttccagcagg tggccagcgg agtccaatca ctcgattccg gcctcccctc cggcgggtgg
     1921 cgcgaaaccg tccttcgcaa cccggcactc ggcccggtct ttcaatgggt ggaatcgtat
     1981 gtggatatcg aggcgcgcct caaagatgcc ggggccgccg ttgccgaacg gatgtcatcc
     2041 tgggtcaccg gttctgtctg ggcaatggcg cagttcttta tcacattctt tctattgttt
     2101 tacttccttc gcgatcgtcg tgccgtactg aacgcccttc gttcgctggt tcccctctcc
     2161 cgcgccgaaa ccgacgaggt gttccaaggc gtggaggact ccatccacgc caccatctac
     2221 ggcaccctca ctgtcgccct catgcagggt gcgctcgggg gattgatgtt ctggtggctc
     2281 ggactccccg cacctttggt ctggggtgtg gtgatggccg gactcgccat catccccatt
     2341 cttggtgcgt ttattgtctg ggttccggcg gcgctgttcc tgctggctgg cggatactgg
     2401 gtcaaagctt tgattctgag cctttggggc gccgttgcta ttggattgat agataacatc
     2461 ctgtatccgg tcctggtagg ctcgcgcctg cggctccata cggtgccggt cttcatcgcg
     2521 gtggttggcg gggtcatcgt cttcggtact tcggggatta tagtcgggcc gattgtcctc
     2581 gcggttacaa tggcgttggt cgatatctgg aaaagacgca ctgccgcata ttccacggcg
     2641 gaagaagcca tcgggaagaa tcttgcgacg ggtcttaccg aaccggaagc caagcctgcc
     2701 aaccccgtta cgctataatc ggtcatttgc gaccaaaccc tcctgaatct ccgtttcaaa
     2761 agcgaagaac aagtgtcgcc gctgccttct ccgcccgcgg cctcgatggc ctgctcgtaa
     2821 cggccctgcc caacatccgg tatctgacga acttcaccgg ttcgaacggc gctctgctcc
     2881 tctttcggga gcacgccatc ctctataccg atccgcgcta tgaattgcaa gcttccgagg
     2941 aggccgattg cccggtgaaa gtcgcccgcg gtccgttatg ggccgccgtg aggaaggatc
     3001 tcagccgcaa gcgtttgaag cgcacggggt ttgaaaaagg ccgcattacg tatgaagcgt
     3061 acgaggccct gcgaggatcg gacgccggcg actccggcct ggttccactg ggcggcatcg
     3121 tggagtattt ccgcatggtc aagtccgaag acgaagtgga agccatccgc cgctcggtcg
     3181 caaccgcctc gaaggctttt cacgaggcgg tgaagcggat ccggcccgaa atgacggaga
     3241 aggaactcgc cgccgaactg gattaccgga tgcggcgcct cggcgcagag aagcccgctt
     3301 ttgagaccat tgtcgcctcg ggtatgcgaa cggctttccc ccacgccaac ccggcatccg
     3361 gtacgatcgg ccgtaatcgt ttagtattaa tagacatggg cgcgagccag aacggctacg
     3421 cgagcgatat gacccgtatg cttttcagcg ggcgtccagg tgcaaagctc caacggcttt
     3481 acaattcggt gctggaggcg cagcttgccg ccatcgccgc ggttcacgag ggggtaacgg
     3541 ctggacaggt ggacgggaaa gcgcggcagg tattaggacg ctacggcttg cgagatgcct
     3601 tcgtacattc caccgggcat ggcctcgggc tcgaaattca cg
//
LOCUS       DASTMK010000620        15893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_447953, whole genome shotgun sequence.
ACCESSION   DASTMK010000620 DASTMK010000000
VERSION     DASTMK010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15893
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447953"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..886)
                     /locus_tag="VFQ79_25615"
     CDS             complement(56..886)
                     /locus_tag="VFQ79_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0143124.1"
                     /translation="MPFTLKGRSAMVTGAGRGIGRAIASSLAAAGASVMINDADETMA
                     REACGALSGSGAKVEYLAGDITVPEFPQQLVDATIGRLGSLDIIVNNAGYTWDNVIQK
                     TTDEQFQAMLDVHVAAPFRVLRAASAWIRETAKKESAAGTRVMRKVVNITSISGTDGN
                     SGQSGYSSGKAAVIGLTKTLAKEWGRYNVTVNAVGFGLIETRLIQPRTSEAPSIEVQG
                     RNIPVGVRPEVLEAARSMCPLGRLGTPEEAAGAVLFFCSPLSDYVTGEVLICGGGMHF
                     "
     gene            complement(1041..2672)
                     /locus_tag="VFQ79_25620"
     CDS             complement(1041..2672)
                     /locus_tag="VFQ79_25620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143125.1"
                     /translation="MNAIREAIARLLRSAWVSGRLRSFGIDPRRYWLLVNLFGELGDR
                     GEMADQLGWNGAALTVVAKLYAVLFGILSLIVAAGGPPLSMYAFGSLAVSTSLLLVVL
                     LSEAGNSLVNPAESLVLAHQPVNGATYIAAKLTHLVRVVLYMATAMNAGPAFAGLLLK
                     ESGWWYPPVHLLAAILSGFVAALLCCALYGWLIRFLPAARLRAAGQFAAAIPFLLIVF
                     SGYVIELVARLGIPRWLPARPSTRWAVAAACGAFVIGIVILGIRSLSADYLVRVSSLM
                     HAGSAAGAKVRRSRIGEAVAWFFGGPAARAGFSFVSRLMLRDWQFRRQFLFAAIGIVL
                     LGVQIIATGWRTDPFSRQFSAAHLLPHLLGALLFMICILLPYTSDHKGVWIFLLAPPL
                     VFGRFARGVHALLWIEIIVIPHIGLLLLFSWVWGIGKAALFIAYSVAATSVYLAIELR
                     VLNGLPFSKPVNPSIGAAMLPMMIMGGVAMGIVVALQYFFLFRSSAIAALAAAVTGAT
                     AWFLTRRNLVAFEDSIHHELGLISAEVPSMFKEVG"
     gene            complement(2669..3445)
                     /locus_tag="VFQ79_25625"
     CDS             complement(2669..3445)
                     /locus_tag="VFQ79_25625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0143126.1"
                     /translation="MPEPVVEVENAVFCYDRTEVLHNISFTLNKGDVAGLLGPNGAGK
                     STTLKLLTGILAPASGRIAVMGLPMPERTVDVKQRIGYVPEAAVLYDSLTGQEFLELS
                     GRLHDVEEETLQNRIRTILETFDLTSDRVARLDTYSKGMRQKILLGAALLHNPEVLLL
                     DEPLSGLDVNTTILVKDLIDALASDGRAILYSSHVLDVVEKVCDRVLIIHKGKLIADG
                     TVDSLKAFGEESSLEDVFRRLTHSEDVNPGVSRILAALRS"
     gene            complement(3463..4890)
                     /locus_tag="VFQ79_25630"
     CDS             complement(3463..4890)
                     /locus_tag="VFQ79_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0143127.1"
                     /translation="MPEFTEDIFFATIGELGAKLRAREFSCVELTRAFCDRLEKLGPR
                     YNALALPLREEAMRQARNVDDDLNRKRFRGPLQGIPYGAKDLLAVAGHITTWGARPFA
                     AQVFDYNATVVNRFQRAHAILIGKLSMVELAGGGGYRYASASLQGSGLNPWDRTRWSG
                     GSSSGSGSAVAAALVPFAIGSETWGSIVTPCAYCGVTGLRPTYGYVSRYGAMALSWTM
                     DKLGPMCRSAEDCGLVLQTIAGGDSNDPGSAGKSFYYAPQYVKKLSDIRVGYAPEDFE
                     ELAYPAARPAFEKALEVVRGLGAQLKEVRLPELPYSMVAGAVVNTEGSAIFEPLIRDG
                     RVDQLEDSKQIAGLKAGLEVKASSYLKAMRLRRKMQDEARRLFGEVDAMLAPSLFGIA
                     NKITQALDDPSQPRPKPKPRGNRDLNTMGNLAGLPELILPCGFAEGMPVAVSLVTRPF
                     NENLILQLGNQFQNATGFHRQRPKV"
     gene            complement(4897..5091)
                     /locus_tag="VFQ79_25635"
     CDS             complement(4897..5091)
                     /locus_tag="VFQ79_25635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143128.1"
                     /translation="MKKLTRRELAAGLAAVSAAQAQQKAKSEPADDLERALKGVRDTG
                     ERLSDFDIPISTEPAFQFKA"
     gene            complement(5123..6631)
                     /gene="aroE"
                     /locus_tag="VFQ79_25640"
     CDS             complement(5123..6631)
                     /gene="aroE"
                     /locus_tag="VFQ79_25640"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU0143129.1"
                     /translation="MSHRTPLPKVCIALGLPTSTELCEHARRELEGGERFFEFRLDYL
                     ERPETGVEAISRFLSEYPDTTILATCRRHQNQGRFNGSIEQQLKILEAAAAAGAKGVD
                     VEIESAESSPSILDPLRGQTFLLISYHNFTGTPPLETLLRRMTRVPADAYKIVTTPRK
                     PSDNYRVLSLPKAHPRVSMVVLAMGEMGFPTRVLSPAFGGLYTYAAPHAAEGTAAGQV
                     SARQLRHCYRVEKFSRTARIYGVIADPVRHSISPAVHNRAFQARRIDAVYLPFLVHPN
                     QLKDFFLLAEKLPVTGFSVTIPHKQRIIRYLDIVDPLARRIGAVNTVWRKGGKWRGAN
                     TDVPGVTVPLSKHLRLPKSSVLLVGNGGAARSAAFALTDAGAKVSIVGRNVDRVRALA
                     KNCGAEPLLLKQAETRNFDAIVHATSVGMYPHSNECFFNGRIPGDVVFDMVYNPLETT
                     LIQRASAEGKTVIPGLQMFLEQAAHQFELWMGESAPRPVMERAALEALAPRT"
     gene            complement(6700..7443)
                     /gene="deoC"
                     /locus_tag="VFQ79_25645"
     CDS             complement(6700..7443)
                     /gene="deoC"
                     /locus_tag="VFQ79_25645"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU0143130.1"
                     /translation="MDEQRQPSATYEELAKMIDHSLLRPELSEQDVAEGCRIAREYGV
                     AAVMLRPSDVDLASRWLAGSGVRLGSVAGFPHGSGTTASKLYEAQDLLRRGVKEVDMV
                     INIGKMISRQFQYVESELLQMANLCHQGGAILKVIFENAYLSEDLKIIACKICKRTDV
                     DFAKTSTGFAPTGATMEDLRLMVRVCGERVKVKAAGGVRTLEKALEVHQVGCARFGAT
                     ATAAILDDWKAHLAAQTAPPAADGLAPVS"
     gene            complement(7558..8367)
                     /locus_tag="VFQ79_25650"
     CDS             complement(7558..8367)
                     /locus_tag="VFQ79_25650"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0143131.1"
                     /translation="MVQLQRDVSLLQDDVRSLQRTVDEKLATLNTLIQQTLDNVNKAN
                     TNVAVLDSNLRDKLRDQEKMVAAPVAGLNSRLEQMASEFQALKETIADMNGRMGKLQA
                     QVTDLGSTIKVLSSPPPPPGSGASSEGPPPGTSAEALYSNALRDQSGGRFDLALQQFT
                     DYVAWFGDTDLAPNAQFYIGSIQYNRGEFDSALKSFDMVLEKYPQNNKTLDARYMKGM
                     SLVKMGQRNAAVQEFRALIKTAPSSEQATKSKTQLNSLGVPYAPAATKKRR"
     gene            complement(8565..8915)
                     /gene="pal"
                     /locus_tag="VFQ79_25655"
     CDS             complement(8565..8915)
                     /gene="pal"
                     /locus_tag="VFQ79_25655"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEU0143132.1"
                     /translation="MSSEVQDVYFDFDESAIREDARATLTRNAEALRSIFGEFADSAV
                     TLEGHCDERGSAEYNLALGDRRASAAKEFLQQLGLSGDRLRTVSYGEERPQCTESSES
                     CWQMNRRVHFSAGQ"
     gene            complement(9454..10833)
                     /locus_tag="VFQ79_25660"
     CDS             complement(9454..10833)
                     /locus_tag="VFQ79_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143133.1"
                     /translation="MTKKRLAALSGTAAFLGLSAALGLVAQQSDILIKITKGERPVIA
                     VPDFRASGEAQQYMGAFNQTLWDDLEKSGVFRMASKSMYPLRVPQQPKDFLSPLPSGT
                     SESSQRGLRMQDWAGPPVNANYMAFGYAAAQGEQLALFGWLYNVNQTTAQAAQVLGKI
                     YLGGLDEAGARKVAHEFAADILGQFGIKALLGSKIYFVSDRTGHKEIWSMDHDGGNQS
                     QLTFFKSISTMPGASPDGSKIAFTTFARGTPQITIFSTASRNRLPFYNQRASLNAFPS
                     FTPDGNQILYSSTATGISQIFIANTDGSGFRRISSVRAIEVEPKVNPKNASEIVFVSG
                     RSGVPQIYRMNIDGADVTRLTTGEGHAVNPAWSPDGQHIAFSWTRGYEPGNFNVFVMD
                     VATRQFVQLTHGVGRNENPTWAPDGRHIVFSSNRSGSAQIWTMLADGTQLQRLTSQGR
                     NEKPVWSAQ"
     gene            complement(10830..11621)
                     /locus_tag="VFQ79_25665"
     CDS             complement(10830..11621)
                     /locus_tag="VFQ79_25665"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0143134.1"
                     /translation="MTGHADILDERESLRRPLAGSVLLHVAVFSSLVAYTWVDSRGRE
                     QFGSPNAGGGSVIVTPVSQINVPSRGGVVNPVANDTESLVPAPPPKPTAQQRQPPPPE
                     PDAIPIKTKRLPRKQSEIAASRQQYRSKQEEQKGQLYSQSGQAASSPMFGSVGGGGSV
                     GGPGGSLGTRFGWYDQLLREKIARTWRTADVDARLQTAPPVIVTFDILRDGSVRNVRL
                     LQRSGNYQLDMSAQRAILEAAPFQPLPPGFERNVAAVEIWFQLKR"
     gene            complement(11632..12081)
                     /locus_tag="VFQ79_25670"
     CDS             complement(11632..12081)
                     /locus_tag="VFQ79_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU0143135.1"
                     /translation="MAISVNGGGWPSRRQRFRTQGTLSEMNVVPLVDVVLVLLIIFML
                     TAHVMEFGLEVDVPKVRQTRDSAEELPVVTVTQNGNLYLNDKLMNINDLGGAIRQQFP
                     GMNAVYVRADRGAVWDPIAQVISELGLAKLEVRVLTQSDEESGKRRK"
     gene            complement(12086..12736)
                     /locus_tag="VFQ79_25675"
     CDS             complement(12086..12736)
                     /locus_tag="VFQ79_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU0143136.1"
                     /translation="MSIWDLVKTTQPIPIAVLSILLIVSVLSWAIIFSKWNMFRQART
                     ANVRFLRAFRKSNGLDQVALASEQFPVSPLVAVFDFGYEEYERQVKSRGALRNLAAVE
                     RSLQLGISEELTKLERNMSWLATAATVSPFIGLFGTVWGIIDAFQALGLAGSASLRAV
                     APGIAHALVATAMGLAAAIPAAVFYNHFGHVIREMANRMEDFSLEFVNFAERVSGE"
     gene            12897..14576
                     /gene="recN"
                     /locus_tag="VFQ79_25680"
     CDS             12897..14576
                     /gene="recN"
                     /locus_tag="VFQ79_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU0143137.1"
                     /translation="MLVELLVENYAVIERARIRFHRGLNLLTGETGSGKSIVVDALGL
                     LFGGRASAESVRSGADRARISGLFEIPEEPGIRRLLEEAGLEPEDGELLLEREILSSG
                     KSRAFAASKPVTVALLRDLAVFLGDIHGQHDQQLLFSPEAQLNIVDQAADNAGLVART
                     AAVYREWRTCARELAELERSGQERLRLTDLWNFQRREIEAAAVRPGEDAELEQERRVL
                     QNVGRLQENVTIAYSLLYDSPDSAAAQLKQAARRLEELHRIDQTFGELLEALKPAAIA
                     LEETSHTLRGYLSHLEADPARLEQVESRLALLEGLKRKYGSTLEEVLAFLETVRGHLD
                     AVEHSGERAEAIRVKQQERACAYEQLARQLSDSRKRAARTLERQVEAELKSLAMERTV
                     FRVQFEQTSWAETGTDRAGFLVSPNVGEEPKPLDKIASGGEISRIALALKTCIAPSVH
                     ANGPQRTLVFDEVDAGVGGRAAETVGRRLKRIAAQNQVLCVTHLPQIASFADHHYVVE
                     KREVKGRTVAEIEEITADARTREIGRMLSGERLTPEALKHAEQLIRLGAEK"
     gene            complement(14587..>15893)
                     /locus_tag="VFQ79_25685"
     CDS             complement(14587..>15893)
                     /locus_tag="VFQ79_25685"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU0143138.1"
                     /translation="GENHRFVAPDYDTVVDSPIVAGNPTIHKFEVGGKPHYLFNFGGD
                     RFWDGGAARDVRRVVESSFRLWGSLPYDKYVFLNVIAEASGGLEHKNSTLLLTSRWAY
                     RVRDAAETGRPNYINWLDLVAHEFFHVWNVKRLRPVELGPFDYENEVYTKSLWVAEGL
                     TSYYAPLIVKRAGLTGTTQFLRLLSRRIETLETTPGRLVQPVETSSYDAWIHLYRPNE
                     NTPNSGISYYTKGAVIGLLLDAKIRSATGGRKSLDDAMRLAYERYSGRSGFTPEEFRR
                     VLAEAAGTDLEGWLKETLETATELDYREMLEWFGLRWQTGTPGESKNQKPTTGLVTRA
                     DGSRLIVNQVRRDTPAYASGISAGDEILAVDGYRVLAEQWPSRMDQYRPGDQIELLIS
                     RRGELKKVSLSLVAESPKKWRLEVKPDATDEQKDRLEKWLSN"
BASE COUNT         3234 a         4838 c         4750 g         3071 t
ORIGIN      
        1 agatggatca catcacttcg tatctgatga tgctgaagtg agagcggtcg cgtgtttaga
       61 aatgcatgcc gccgccgcag atcaatactt ccccggtgac gtaatccgag aggggagaac
      121 agaagaacag cacggcgccg gcggcttctt cgggcgttcc aagccggccg agcggacaca
      181 tggaccgggc tgcttcgaga acctcgggcc tgacgccgac gggtatgttc cgtccctgta
      241 cttcgatgct tggcgcctcg ctggtgcgcg gctgaatgag ccgggtctcg atcaggccga
      301 agccgactgc attcacggtg acgttgtagc ggccccactc cttcgccagc gttttcgtta
      361 atccaatgac cgccgctttg ccggaagagt atccggactg gcccgaattg ccgtccgtcc
      421 ccgaaataga ggtgatgttg acgaccttcc gcatcacacg cgtgcccgcg gccgattcct
      481 tctttgccgt ttcccgaatc cacgcggagg cggcgcgcag tacgcggaag ggagccgcca
      541 cgtggacgtc gagcatggcc tggaactgct catcggtggt cttctgaatg acgttgtccc
      601 acgtataccc ggcgttgttg acgatgatat cgagggaacc caaccggccg atcgtggcat
      661 cgacgagttg ttgcggaaac tccgggacgg tgatgtcgcc cgcgaggtat tcaaccttcg
      721 ccccgcttcc cgaaagcgcg ccgcaagcct cgcgcgccat cgtttcatcg gcatcgttga
      781 tcatcaccga agccccggcg gcggcaaggc tggacgcgat cgcccgtccg attcctctac
      841 cggccccggt aaccatggcg cttctaccct tcagcgtaaa cggcatagcg atgtccagtc
      901 gacctcctct ccggctaagt actcctaatc ggaattacgg taaccgctac ccgcttgctg
      961 gcgcgcgcgg ctccgtttcc gagccacgac cgcaagggag cggtttcatt gaaacgtgag
     1021 tgaacttgtg aaaccatgta ctaacctacc tccttgaaca tcgaaggcac ctctgccgaa
     1081 atcagcccga gctcatggtg aatcgaatct tcaaaagcga cgagattgcg gcgcgtcagg
     1141 aaccaggccg tagcgccggt cacggcggct gccagcgccg caatcgcgga cgaacgaaac
     1201 aggaagaaat actgtagcgc cacgacaatt cccatcgcga ctccacccat gatcatcatc
     1261 ggcaacattg cggcgccgat ggatggattc accggtttgc tgaacgggag gccgttcagc
     1321 actcttagct caattgcgag atagacagac gtcgcggcca cgctgtaagc aatgaacagc
     1381 gcggccttcc cgattcccca cacccatgaa aaaagcagga gcagaccgat gtgcgggatg
     1441 acgatgattt cgatccacag cagcgcgtgc acgccgcggg caaagcggcc aaataccagc
     1501 ggcggcgcca gcagaaaaat ccagactcct ttgtgatcgc tggtataggg cagcaggatg
     1561 cagatcatga agagcagagc tcccaacagg tgtggcagca aatgggcggc cgagaactgg
     1621 cgtgagaacg ggtccgttcg ccaaccagtc gcgatgatct gcacgcccag taatacgatt
     1681 ccgatcgccg cgaagaggaa ttgccggcgg aactgccagt cgcgcagcat caggcgcgag
     1741 acgaagctaa accccgcccg ggctgccggc ccgccgaaga accaggcgac agcttcgccg
     1801 atccgcgatc gccttacttt ggcgccggcg gcggaacccg cgtgcataag actggacacg
     1861 cgcacgagat aatcggccga gagagaacgg atgccgagaa tcacaatgcc aatcacgaag
     1921 gcgccgcatg cggccgcgac cgcccaacgc gtggaagggc gggccggcag ccagcgggga
     1981 atgccgagcc gggcaaccaa ctcgatcaca tacccggaga agacaatcaa caggaacggt
     2041 attgccgcgg cgaactgtcc cgcggccctt aaacgcgccg cgggcagaaa ccggatcagc
     2101 cagccgtaca aggcgcagca aagtagtgcc gctacgaagc ccgacagaat cgcggcgagc
     2161 aggtgaaccg gtggatacca ccatcccgac tccttcaaca gaagaccggc gaaggccggg
     2221 ccggcattca tcgctgtcgc catgtacaga acaacgcgaa ccaggtgagt cagcttggcc
     2281 gcaatataag tagctccatt aacgggctgg tgcgccaata ccaggctctc cgccggattc
     2341 acgaggctgt ttccggcttc tgagagcaga acaacgagca gaagagaggt gctgacggca
     2401 agagagccga aggcatacat cgaaagaggc ggaccacccg cggccacgat cagactcagg
     2461 atgccgaaga ggacagcata gagcttggcc acgaccgtga gagccgctcc gttccagccc
     2521 agttgatccg ccatttcacc gcgatcgcca agctcgccga acaggttcac caggagccag
     2581 taacggcgcg gatcgatgcc gaagctcctc aagcgccctg acacccaggc gctgcggagc
     2641 agacgtgcta tcgcttcccg gatcgcattc atgaccgcag agccgccagg attcgagaga
     2701 caccggggtt cacatcttcc gaatgtgtaa gcctgcggaa gacatcttcg agcgacgatt
     2761 cctccccgaa agctttgagg ctatccacgg taccgtccgc gatcagcttg cctttgtgaa
     2821 tgatcagcac acggtcacag accttttcca cgacgtccag cacatgggag ctgtacagga
     2881 ttgctctccc gtcggatgcc agggcgtcga tcaggtcttt gaccagaatg gtcgtgttga
     2941 catcgagtcc cgagagcggc tcatccagca aaaggacttc cggattgtgc agcaaagccg
     3001 cccctaacag gatcttctgc cgcatgcctt tcgagtaggt gtccaggcgg gcgacgcgat
     3061 cggatgttag atcgaacgtt tcgaggatcg tgcggatcct gttctgaagc gtctcttcct
     3121 ccacgtcgtg caggcgaccc gatagctcca gaaactcctg cccggtaagg ctgtcgtaaa
     3181 ggacggcggc ttcgggaacg tagccgatgc gctgttttac atcgacggtc cgttccggca
     3241 tcgggagccc catgacggcg attcttccgg acgccggagc cagaatgccg gtgagcagct
     3301 tgagtgtcgt gctctttccg gcgccattgg gtccgagcaa accggcgacg tcccctttgt
     3361 tcagcgtaaa ggagatgtta tgcagcactt ccgtccggtc gtagcagaaa acggcgttct
     3421 caacttcaac gacaggctcc ggcataaacg gaccatttaa tcctacacct tggggcgctg
     3481 ccggtggaag ccggtggcgt tttgaaactg gtttccgagt tggagaatca ggttctcatt
     3541 gaacgggcgg gtgacaagcg acacggcaac gggcatgccc tccgcgaagc cgcacggcag
     3601 gatgagttcg ggtaagcccg cgagattccc catggtattc agatcgcgat tgcccctcgg
     3661 tttcggcttg ggccggggtt gggatgggtc gtcgagagct tgcgtgatct tgttcgcgat
     3721 tccgaacagg ctgggcgcga gcatggcgtc cacctcgcca aagaggcgcc gtgcttcatc
     3781 ctgcattttc ctgcgcaggc gcatggcttt gagatagctg gaagccttca cctccagccc
     3841 ggctttaagc ccggcgatct gcttactgtc ttcgagctgg tcaacacggc cgtcgcggat
     3901 cagcggttca aaaatagcgg agccttcggt gttgacaacc gcaccggcca ccatgctgta
     3961 aggcagttcg ggcagcctca cctctttcag ttgggcacct agcccgcgca ccacttcgag
     4021 agctttttca aacgcggggc gtgccgccgg atacgccagc tcctcaaaat cctccggcgc
     4081 gtatccgaca cgtatgtcgc tgagcttttt cacgtactgc ggcgcgtaat aaaaactctt
     4141 gccggccgaa ccggggtcgt tggaatcgcc gccggcgatt gtttgcagca ccaggccgca
     4201 atcttccgcg gaccggcaca tgggcccgag cttatccatc gtccaggaaa gcgccatcgc
     4261 accgtagcgg ctcacgtatc cgtaagtagg ccgcaggccg gtgacgccgc aataggcgca
     4321 aggcgtgacg atggaccccc acgtctccga cccgatggca aacgggacca gagccgcggc
     4381 gacagcgctg cccgatccgc tggacgagcc tcccgaccag cgggtccggt cccaagggtt
     4441 gagcccggac ccctgaagag aagccgatgc atagcggtag cctccgcctc cagccaattc
     4501 caccatggac aattttccga tgaggatggc atgcgcgcgc tggaagcgat ttacgaccgt
     4561 agcgttgtag tcgaacacct gcgcggcgaa gggccgtgca ccccacgtgg tgatgtggcc
     4621 ggcgacagca agcaggtctt tcgctccgta aggtatgccc tgaagagggc cccggaagcg
     4681 cttccggttg aggtcgtcat ccacgttccg ggcctggcgc atcgcctctt cccgcagtgg
     4741 caaagcgagg gcgttgtagc gcgggcccag cttctccagg cgatcgcaaa aagcgcgcgt
     4801 caattccacg caggagaact cacgtgcgcg cagctttgca ccgagttcac cgatggtggc
     4861 gaagaagatg tcttccgtga attcgggcat cccgtctcaa gccttgaact gaaacgccgg
     4921 ctccgtggaa atgggaatat cgaaatccga caacctctcg ccggtatccc ggacgccttt
     4981 gagcgcgcgc tccagatcgt ccgcgggttc agacttcgcc ttctgctgtg cctgcgccgc
     5041 agatacggca gcgagaccag ccgccagttc acggcgtgtg agctttttca ttcagtagct
     5101 attatgcaca gatggaacgg gatcaggtgc gaggcgccag agcctccagc gcagctcttt
     5161 ccataacggg ccggggcgcg gattctccca tccagagttc gaattgatgg gccgcttgtt
     5221 cgaggaacat ttgaaggccc ggaatgaccg tcttgccctc ggcggaagcg cgttgaatca
     5281 gcgtggtttc aagcgggttg taaaccatat cgaacaccac gtcgcccgga atgcgcccgt
     5341 taaagaaaca ctcgttcgag tgggggtaca tgccgaccga agtcgcgtgc acgatggcgt
     5401 cgaagttccg cgtttcggcc tgtttcaaaa gcagcggctc ggccccgcag ttctttgcca
     5461 gggcacgcac acggtcgacg tttcttccca cgatggacac cttggctccg gcgtcggtaa
     5521 gggcgaatgc ggcactgcgg gccgcgcctc cattccccac cagcagaacg gaagactttg
     5581 gcagacggag atgtttcgag aggggcaccg tcacaccggg aacgtccgtg ttcgcaccac
     5641 gccactttcc ccccttccgc cagacggtat tgacggcacc gatgcgcctg gcgagcgggt
     5701 ccacgatatc gagatagcgg atgatcctct gcttgtgcgg aatggtcacg ctgaaaccgg
     5761 tgacgggcag tttctccgcc agcaggaaaa agtctttgag ctggttagga tgaaccagga
     5821 acggcaggta gacggcatcg atccggcgcg cctggaatgc gcgattatgc acggccgggg
     5881 aaatcgagtg ccgcaccggg tcggcaatga cgccgtagat gcgggcggtg cgggagaact
     5941 tctcgacgcg atagcagtgg cgcaattggc gcgcgctgac ctgaccggct gcggtacctt
     6001 cggcggcatg aggggcggca taggtgtata gtccaccgaa ggcgggagaa agaacgcggg
     6061 tcgggaaccc catttcaccc attgcaagca ctaccatcga tacccgggga tgagccttgg
     6121 gcagcgaaag tacgcggtag ttatcggacg gtttccgggg agtcgtaacg atcttatagg
     6181 catccgccgg aactcgcgtc atcctgcgca gcaaagtctc cagcggtgga gtaccggtga
     6241 aattatgata cgagatcaac agaaacgtct ggcctcgtaa gggatcgagg atcgagggag
     6301 aactctcagc cgactcgatt tcgacatcca ctccctttgc gccggcagcg gcagcggctt
     6361 cgagaatttt gagctgctgc tcaatgctgc cgttgaatct accctgattc tgatggcgcc
     6421 ggcaggtggc gagaatcgta gtatccggat actcgcttaa aaaccggctg atggcctcca
     6481 cgccggtttc aggccgttcg aggtagtcga gccggaattc aaagaaccgc tctccgcctt
     6541 cgagctcccg ccgtgcgtgt tcacacagct ccgtgctggt gggaagtcct agggctatac
     6601 agaccttcgg cagcggggtg cgatgggaca tttcctgcga ggtctttaga atagcacggc
     6661 cgccagcggc cgacgttgag gccggaagat gctaggatgc taggacaccg gtgccaggcc
     6721 atccgcggcc ggtggcgcgg tttgagccgc aaggtgagct ttccagtcgt cgagaatagc
     6781 agccgtggcg gtggcgccga aacgcgcgca gcccacttga tgaacttcca acgctttttc
     6841 caacgtgcgc acaccgccgg cggcctttac cttcacccgc tcgccgcaga cgcgaaccat
     6901 caggcgcagg tcttccatcg tcgctcccgt tggcgcaaac cccgtggacg tctttgcaaa
     6961 gtccacatcc gtccgcttgc agattttgca ggcgatgatc ttgaggtctt ctgaaagata
     7021 ggcgttttca aaaatgactt tcagtatcgc gccgccctgg tggcaaagat tggccatctg
     7081 gagaagctcg gattcgacat actggaactg gcgcgagatc atcttgccga tgttgatgac
     7141 catgtccact tccttgacgc cgcgccggag cagatcctgc gcttcatata gcttggaggc
     7201 ggtagtgcca gagccatgcg ggaagccggc aacgcttccc agccggacgc cgcttccggc
     7261 taaccaacgc gaggcaagat ccacatcgga aggtctcaac atgaccgccg ctactccgta
     7321 ctcgcgggct atgcggcaac cctcggccac gtcctgctcg gaaagctcgg gccgcaggag
     7381 ggagtggtcg atcatttttg cgagttcttc gtaggtggcc gacggctgcc gttgctcatc
     7441 catgaaacct cctcccggac cgcttcaaaa agtcacgacc gcttgcttac gcgcgcggct
     7501 ccgtttctga gccgcgaccg cgagggagcg gggctgccat gcgttttcaa caagctctta
     7561 gcgccgcttc ttggtagcag cgggtgcata tggcactccc aggctgttca actgcgtctt
     7621 cgacttggtc gcctgctcgc tcgaaggcgc cgtcttgatg agggcgcgaa actcctggac
     7681 cgcggcattc cgttgtccca ttttcaccag ggacattcct ttcatgtacc gagcatccag
     7741 agtcttgttg ttctgcggat acttctccag aaccatatcg aacgacttca gcgcggagtc
     7801 gaactcgccg cgattgtact gaatactgcc gatatagaac tgcgcgttcg gcgccagatc
     7861 cgtgtcgccg aaccacgcga cataatcggt gaactgctga agtgccagat cgaaccgccc
     7921 accggactgg tcccgcagcg cattgctgta tagcgcctcg gcggacgtgc ccggtggagg
     7981 tccctccgag gacgcgccgg accccggggg aggaggagga ctggaaagca cctttatcgt
     8041 acttcccaaa tccgtaacct gcgcctgcag cttacccatc cgcccgttca tgtccgcgat
     8101 cgtttccttc agcgcctgaa attccgaagc catctgttcc agcctcgagt tgagtccggc
     8161 aacaggcgcg gcaaccattt tttcctgatc gcgcaacttg tcgcgaagat tactgtcaag
     8221 cacagccacg ttggtgttgg ctttgttcac gttatcgagc gtttgctgga tcagcgtgtt
     8281 taacgtggca agcttttcgt cgacagttcg ctggagtgaa cggacgtcgt cctgcaggag
     8341 cgaaacgtct ctctgcaatt gaaccatttc cttgctcgcg ccgaagacag gagcagagac
     8401 cgcgaacaat gcaagtacaa acgggcaaaa aactctcatc gcattcccca ataaacaacg
     8461 tcacaaccgg cgcgccacgg aaacagccgt agcgcgccgc tgtgctttcc gacaaacggc
     8521 ggggacggct cgccgtcgga gcaagccgcc cctgatcgat accgctattg gccggcgcta
     8581 aaatgcacgc ggcggttcat ctgccagcag gattcgctcg actccgtgca ctgcggccgc
     8641 tcctctccgt agcttaccgt tctcagacgg tcaccggaaa ggccgagttg ctgcagaaat
     8701 tccttggcgg cactggcacg gcggtcgccg agcgcaaggt tatactccgc tgagccacgt
     8761 tcatcgcagt gcccctccaa ggttacggcg gaatccgcaa actcaccgaa gatagatcgc
     8821 agagcttcgg cattcctcgt cagcgtcgcg cgggcatctt cacggatggc actctcgtcg
     8881 aagtcgaagt acacatcctg aacttcagaa gacaaccgct cgctcaatgt ccgtttcggt
     8941 gccgccggtg gaggaggagg cggttgcgga acagagacgt ttaccgtggc actcgcggtg
     9001 gcggtacctc ccggaccggt ggcactcaat gtgtaggttg tagtattggt cgggaatacc
     9061 tgacggctac ccgtggtttg aacggctccg acaccgccgc tgatggaaac atccgtggca
     9121 tccgtgactt cccaacggag cgtcgcggat tggccccgct cgatagcaga gggttcagcg
     9181 gtaaagtttt tgatcgtcgg ccttgccggc ggtttaggtt gttcttgcgg gggtgggggt
     9241 ggcggcggcg gtggtggggg tggtgccttt ttcttacatc cagccgcaaa aagcaacaac
     9301 gacaacgcca aaagtaagta tccaatgttt cgcttgctca tcgtgaacgt tagcctcctg
     9361 aaaactcgtg atcgcatgtt ttattccggc tgcatcgcgg cccccgtcgc agggggcgcc
     9421 gattgcagga aaactgatta tattatcttt tctttattgt gcactccaca cgggcttctc
     9481 gttccgcccc tgggaagtga gccgttgcaa ctgcgtgcca tcggccagca tggtccagat
     9541 ctgcgccgaa ccactgcggt tggacgaaaa gacgatgtgc ctgccgtcag gcgcccaggt
     9601 ggggttttca ttgcgtccca ctccgtgcgt caactgcaca aactgccggg tagccacatc
     9661 catcacaaac acgttgaagt tgccgggctc gtaacctctt gtccaagaga aggcgatgtg
     9721 ctggccgtcg gggctccagg ccggattcac cgcatggccc tctcccgtcg tcaatcgcgt
     9781 cacatcggct ccatctatat tcattctata tatttgtggc acgccggacc ggccggagac
     9841 aaaaacaatt tccgaggcgt ttttgggatt taccttaggt tctacctcaa ttgcccgtac
     9901 agaggagatc cggcggaaac cgctgccgtc ggtgttcgcg atgaaaatct gcgagatgcc
     9961 tgtggccgtc gaggagtaca ggatctgatt tccatccggc gtaaagctcg gaaaggcatt
    10021 cagcgacgcg cgctggttgt aaaacggcag cctgttgcgg ctggcggtgg agaatattgt
    10081 aatctgcggt gtgccgcgcg caaatgtagt gaaggcgatc ttcgagccgt cgggcgacgc
    10141 acccggcatt gtgctgatgg acttgaagaa cgtaagctgc gactggtttc caccgtcgtg
    10201 gtccatcgac cagatctctt tatgcccggt gcgatcggaa acgaaataaa tcttagaccc
    10261 gagcagcgct tttatcccga attgtccaag aatatccgct gcaaattcgt gagctacttt
    10321 tcgtgcgccg gcctcatcca acccgccgag atagattttg cccagcacct gcgcggcctg
    10381 cgccgtggtt tggttgacgt tgtacagcca gccgaagagc gcaagttgct ctccctgcgc
    10441 cgcggcatag ccgaatgcca tgtaattcgc attcacggga gggcccgccc agtcctgcat
    10501 tctgaggccc cgctgcgacg actcggaggt gccggaaggg agcggcgaca aaaaatcttt
    10561 cggctgctgc ggcactcgaa gcgggtacat gctcttcgag gccatgcgaa agacgcctga
    10621 tttttcgaga tcgtcccata gcgtctggtt gaaggcgccc atatattgct gcgcctcgcc
    10681 agaggcacgg aaatcgggca cggcgatcac cggacgctcg cccttcgtaa tcttgatcag
    10741 aatgtcgctt tgctgagcga caaggcccag cgcagcggac aagcccagga acgcggccgt
    10801 gccggacaac gctgcgagtc ttttctttgt catcgcttta actgaaacca gatctccact
    10861 gccgccacgt ttcgctcgaa gccgggcggc agcggttgaa acggagcggc ttccaggatg
    10921 gcccgctgag ccgacatgtc cagttgataa ttaccgctgc gctgcagcag cctgacattt
    10981 cggaccgatc cgtcgcgcag aatatcgaag gtgacgatca ccggcggagc ggtctgcaac
    11041 cgggcgtcga catcggccgt ccgccaagtg cgcgctattt tttcgcggag caactgatcg
    11101 taccagccaa accgcgtgcc aagcgaaccg ccggggccgc cgacactgcc cccgccgccc
    11161 accgaaccga acatggggga agaggccgcc tgcccggact ggctgtataa ctggcccttc
    11221 tgttcctcct gcttgctccg gtattgctgg cggctggccg cgatttcgga ttgctttcgc
    11281 ggcaggcgct tggtcttgat gggaatggcg tccggctcgg gaggcggcgg ctgccgttgc
    11341 tgcgctgtgg gttttggcgg aggagccggc accagcgatt ccgtgtcgtt tgctacagga
    11401 ttcacgaccc caccgcgcga gggcacgtta atctggctga ccggcgtgac gataacggac
    11461 ccgccgcccg cgttgggcga accgaactgt tcgcggccgc ggctatcgac ccacgtataa
    11521 gccaccagcg aggaaaagac ggcaacatgc agcagcacag aacccgccag cggtctccgc
    11581 aaagactcgc gttcgtccag gatgtctgca tgtcccgtca tttccttccc gctacttccg
    11641 gcgcttcccg ctctcttcgt ccgactgggt caacacccgc acttcaagtt ttgccaggcc
    11701 cagttcactg atcacctgcg caatagggtc ccaaacggcg ccccggtccg cgcgcacgta
    11761 tacggcattc atgccgggaa actgctggcg tatcgcgccg ccgaggtcgt tgatattcat
    11821 cagcttgtca ttgaggtaga ggttgccgtt ctgagtaacc gtgacaaccg gcagctcctc
    11881 cgcgctgtcc ctggtctgac gcaccttcgg tacatccacc tccaggccaa actccatgac
    11941 gtgcgctgta agcatgaaaa tgatgagcag cacgagaacg acgtccacca gcgggacgac
    12001 gttcatttcc gaaagcgttc cctgcgtccg gaacctttgg cgccgcgaag gccagccgcc
    12061 gccgttcacc gaaatcgcca tggagctact cccccgacac ccgctccgcg aagttgacga
    12121 attcgagcga aaagtcttcc atgcggttcg ccatctcccg gattacatgc ccgaaatggt
    12181 tgtagaacac cgcggcggga atagcagccg ccaggcccat cgcggtagcc accagcgcgt
    12241 gtgcaatgcc gggcgcgacc gcgcgcaaac tggcgcttcc cgccaacccg agcgcctgga
    12301 aagcgtcgat gattccccag accgtgccga acagtccgat gaacggagac acggtagccg
    12361 cggtcgccag ccaactcatg ttccgctcca acttcgtcag ttcctcactg atgccaagct
    12421 ggaggctacg ctccaccgcg gccagattcc gcagcgcacc gcgcgatttc acctggcgct
    12481 cgtactcctc gtagccgaag tcgaatacgg ctaccagggg cgaaacgggg aactgctcgc
    12541 ttgccagggc gacctgatcg aggccattcg acttacgaaa cgcgcggaga aaacgcacat
    12601 tggccgtcct ggcctgccgg aacatattcc atttggaaaa tatgatggcc caggacaaaa
    12661 cggaaacaat cagcagtatg gagagcacgg cgatggggat cggttgcgtg gttttgacca
    12721 gatcccagat gctcacctgg agatacagtg cgagagccgg gggcagtgtt ggactccttt
    12781 caccctaagt atttatcata tccgacacgc cttccgccgc actaaaaaac agattgagca
    12841 ttttttgttt ttttgtgccg ggtgcggggg gccgcgccgg tctataatga ccacgaatgc
    12901 tggttgagct gctggtggaa aactacgccg ttatcgaacg cgcccgcatt cgcttccacc
    12961 gcggcctgaa tctgctgacg ggggagaccg ggagcgggaa gtccattgtt gtggacgcgc
    13021 tgggcttgtt atttggcggg cgcgcttccg ccgagtccgt gcgttcggga gcggatcgcg
    13081 cccggatttc gggactgttc gaaatcccgg aggagcctgg catccgccgc ctgctcgaag
    13141 aggcggggct tgagcccgaa gatggagaac tgctgctcga gcgggagatt ctgagcagcg
    13201 gcaaatcgcg ggcgttcgcc gccagcaagc cggtaacggt ggccctgctt cgcgatctgg
    13261 ccgttttcct cggcgacatc cacggccagc acgaccagca gttattgttc tcgcccgaag
    13321 cccaactgaa catcgtcgat caggcagcgg acaacgccgg actcgtcgcg cgcacggccg
    13381 ccgtctatcg ggaatggcgc acttgcgcgc gtgaactggc ggaactggaa cgttccgggc
    13441 aggagcggct gcggctcacc gacctgtgga actttcagcg gcgcgagatt gaagcggcgg
    13501 cggtccggcc cggtgaagat gccgaactcg aacaggaacg ccgcgtcctg caaaacgttg
    13561 gaaggctaca ggagaacgtc accatcgctt attcgctgct ctacgattct ccggattccg
    13621 cggcggcgca actgaagcag gccgcgcggc ggctggagga attgcaccgc atcgaccaga
    13681 ccttcggcga actgctggaa gccctgaaac cggcggcgat cgcgctcgaa gagacgtcgc
    13741 acaccctgcg cggctatctc tcgcacctcg aagcggatcc cgcgcgtctg gagcaggtgg
    13801 agtcgcggct tgccctgctc gaaggattaa agaggaagta tggctcgact ctggaggagg
    13861 ttctcgcgtt cctcgaaacc gttcgcggcc atttggacgc cgttgaacac tccggcgaac
    13921 gcgccgaagc gatccgggtc aaacaacagg agcgggcatg cgcttacgag cagctagcgc
    13981 gccagctttc agacagccgg aaacgcgcgg cacgcacgct ggagcggcaa gtggaggccg
    14041 agctgaaatc ccttgccatg gagcgcaccg ttttccgcgt tcagtttgag cagacatcct
    14101 gggcggaaac aggcacggac cgtgccggct tccttgtttc cccaaacgtg ggggaggaac
    14161 cgaaaccgct cgacaaaatc gcttcgggcg gtgaaatttc gagaatagcc ctggcgctga
    14221 agacctgcat cgcgccctct gtccatgcga acggccctca gcggacgctg gttttcgatg
    14281 aagtggacgc gggggtcggc gggcgggccg ctgaaacggt gggacggcgg ctgaagcgca
    14341 ttgccgcgca aaatcaggta ctgtgcgtaa cacacctgcc gcaaatcgcg agcttcgcgg
    14401 atcaccacta tgttgtggag aagcgcgagg tcaaaggaag aaccgtggct gaaatcgaag
    14461 agatcaccgc ggacgcgcga acccgggaga tcggccgcat gctgtcgggc gagcggctga
    14521 cgccggaggc gctgaaacac gcggaacaac tcatccggct gggtgcagag aagtaaacac
    14581 tgctcctcag ttcgacaacc atttttcgag ccggtccttc tgctcatccg tcgcatccgg
    14641 cttcacctcg aggcgccact tcttcggaga ttcggccaca agagaaaggc tgaccttctt
    14701 gagttcgccg cggcgcgaga tcagaagctc aatctggtcc ccgggcctgt actggtccat
    14761 gcgcgacggc cactgttccg ccagaacccg gtaaccgtcc accgcaagaa tctcatctcc
    14821 agcggagata ccggaagcat acgcaggagt gtctctcctc acctggttca ctatcaatcg
    14881 cgagccatcg gcgcgggtga cgagtcccgt cgtgggcttt tgattcttac tttcgccggg
    14941 cgttccggtt tgccaacgca acccgaacca ttcgagcatc tcccggtaat cgagctcggt
    15001 ggccgtctcc agtgtttcct tcagccagcc ttccaggtct gtgcctgccg cctcggccaa
    15061 cacccgccgg aactcttccg gagtgaagcc gctccggccg gaatagcgtt cgtaggccag
    15121 ccgcatggca tcatcgagac tcttgcgtcc gcccgtcgcg ctgcggatct tcgcatccag
    15181 caatagtccg atcaccgccc ctttcgtgta atagctgata ccgctgttcg gcgtattttc
    15241 gttggggcgg taaagatgaa tccaggcgtc ataggaagac gtctcgacgg gctggaccag
    15301 cctgccgggc gtcgtttcca gcgtttcaat acggcgggac agcaggcgca ggaattgcgt
    15361 tgtgccggtc aggccggcgc gcttgacgat cagaggcgcg tagtaactgg taagaccctc
    15421 ggctacccaa agcgatttcg tatagacctc gttttcgtaa tcgaatggtc cgagctcaac
    15481 cgggcgcagc cgtttcacat tccagacatg gaaaaattcg tgggcaacca ggtccagcca
    15541 gttgatgtag ttcggccggc cggtttcggc ggcatcccgg acgcggtagg cccaacggct
    15601 ggtcaacaac agagttgagt tcttatgttc gagaccgccg gacgcttccg cgatcacatt
    15661 gaggaagacg tacttgtcgt agggtaaaga gccccacaac cggaaactgc tttccacgac
    15721 gcggcggacg tcccgcgcgg ctccaccgtc ccagaaacgg tctcccccga aattgaacag
    15781 gtagtgcggc ttgcctccaa cttcgaattt gtggatggtg ggattgcccg cgacgatggg
    15841 cgaatcgaca accgtatcgt agtccggcgc gacgaagcga tgattctccc cgc
//
LOCUS       DASTMK010000621         4563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U3627
            k141_95961, whole genome shotgun sequence.
ACCESSION   DASTMK010000621 DASTMK010000000
VERSION     DASTMK010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970058
            Sequence Read Archive: SRR11285378
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:53:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,110
            Genes (coding)                    :: 5,090
            CDSs (with protein)               :: 5,090
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95961"
                     /isolate="SMAG_U3627"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.385248 N 111.02402 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1424)
                     /locus_tag="VFQ79_25690"
     CDS             complement(168..1424)
                     /locus_tag="VFQ79_25690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143139.1"
                     /translation="MRKLRDFGTSFVQLLCRTRLHSSEVLLHRRALKHTWTFPTISVG
                     TANSVTMTLTNNLGTPGNEGDWEMIDSVSPVMTEGARMWIRDLAREFQQADVNASFAF
                     SMECYNPPMPMRARYLSLVDGVVQPGVDVYLEIPSHQMHFGSRVRNYLRQVYKECADE
                     IAAAGLPVILQFGETQYWYFDNRAQDPNGGMGMQDQEAIDAFAARYGHQIWPFRANTD
                     DPAGDPLYPFETANFLRDRIWEYCQDVIGYVRQYHPAALFECLWPLDANQGKPAPHAQ
                     HRRLLMHVNLPEQWKNSSYGVKYFRCEGFDYDVWQKNAVRMRETLAFPREVLGRPVEE
                     CLYLAGIYGPPDPPIAQAFGMWRAAKLASMCFWAVDQFCLNGRPVPFPVWTQSKATAV
                     AYHKPRAARAAGVLNRCKSNQRRLNE"
     gene            1362..1556
                     /locus_tag="VFQ79_25695"
     CDS             1362..1556
                     /locus_tag="VFQ79_25695"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0143140.1"
                     /translation="MQTRAAQQLYKRRSEIAEFPHMWAKAVKKWTRFSVRGLAKAGME
                     ALWLAFAYNISQWSTLARAA"
     gene            1794..2585
                     /locus_tag="VFQ79_25700"
     CDS             1794..2585
                     /locus_tag="VFQ79_25700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143141.1"
                     /translation="MAIYTSFEMVEDCKAGLRRGWVYFVSEFEPVIAALAAHYCAERY
                     ASKPSTDFLRDLHTGEFFQSVRPATQREFAIELRQAVLALLEAACPTPAPDIELSLED
                     VTTALAPLTPVEKQMAWLETMKYEPAHAALTMNAGTDTVERLREKVEELLRQSLDRWK
                     RGLLRENGLQLRAEAAARSSQDCVPVKLFLDILDGRVTWSNRQNIDRHLASCWHCIDR
                     FCRTREIDRLVKDTPPLTAEGTDAHLEKLGFPKEKAPFWKRILAR"
     gene            complement(2619..4082)
                     /locus_tag="VFQ79_25705"
     CDS             complement(2619..4082)
                     /locus_tag="VFQ79_25705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0143142.1"
                     /translation="MQDDWKVSEKLTVNAGLRYDIMTPARDADDRLANFDPSTNSLKM
                     ASGDDRSLQRQPYNNFAPRLGAAYRLNANTVIRGGYALGWNFWNRMASAEYLNTNAPY
                     VTRFSTVNSAANLGNICTGNSYSGCFRPREAGYPGDLPSNVILYMDPDTPWGYVQNWH
                     FTIRRTLFKDTLLDVAYVGNRGSRLPVLGDLNQARPITQQKLSAGLTTLVTLLARRPY
                     QGWNNITAVVPDGFSRYHGLQMKFEHRGRDLSVISSFTYSKAIDTVGQVLETTNGGSP
                     NPQDIHNIRNDKGASSFDQRFNSTTSLVYNLPFGKGRRFATSMPAVVEGIVGGWQVSG
                     IVTLLSGQPLNIRYPDSSGILSDGQADFLGNVALRPNYLGGKIKNDDSTMERHLAYFN
                     RSALAIPPVTSPFGNLGRNVVYGFPLYQTNMVFAKNFILPFINEGARLQFRGEFYNLF
                     NKTNFTAPTVDLSSANFGRVSSTLDPRFVQLALKLLF"
     gene            complement(4016..>4563)
                     /locus_tag="VFQ79_25710"
     CDS             complement(4016..>4563)
                     /locus_tag="VFQ79_25710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0143143.1"
                     /translation="VEYRLGITRLGMDRTPAAVGGPSMEELFGITGLPTGDKVRGGIT
                     PQDINGFPRYGRQSTNPQAQFPTSINSRLNYSRTAGRHNLKTGYEWLGLYITVDDTNP
                     LYVIDSFGGAYSRPSTGVPSGVNNSWYHLADFFFGARSQYQLATQVQARVRQHATSFM
                     CRTTGKLVRSLLSTPGCGTTS"
BASE COUNT          930 a         1324 c         1430 g          879 t
ORIGIN      
        1 tggacagaat gccgtagctg gcggcgaagc cgttcgtgga accaacattg atggtcgagt
       61 caccgacaag gaccgtctgc ggggtcgtcg tctccagtgg aaccgtcgaa aacgcgtcca
      121 caggcgagta cagcgtcgcc gcgttgtcga tgttgttggg atagctgcta ttcattcagc
      181 ctcctctgat tcgacttgca ccgattgagc accccggcgg cgcgcgccgc gcggggcttg
      241 tggtacgcca ccgctgtcgc cttcgactgc gtccacaccg gaaacggcac cgggcggccg
      301 ttcaggcaga actggtcgac ggcccagaag cacatcgagg cgagcttcgc cgcccgccac
      361 ataccgaacg cctgcgcgat cggagggtcc ggaggcccgt agatgcccgc caggtacaga
      421 cactcctcga ccggacggcc gagcacttcg cgcgggaacg cgagcgtctc gcgcatccgg
      481 actgcgttct tctgccacac gtcgtagtcg aagccttcgc aacggaagta cttcacgccg
      541 taggacgagt tcttccactg ctcgggcagg ttcacatgca tcagcaatcg gcggtgctgc
      601 gcatgcggag ccggcttgcc ttgattcgcg tcgagcggcc acaggcactc gaacaacgct
      661 gccggatgat actggcgtac gtaaccgatc acgtcctgac agtattccca gatgcggtcg
      721 cgcaggaagt tggccgtctc gaacgggtag agcgggtcgc cggccggatc gtcggtgttc
      781 gcgcggaacg gccagatctg atggccgtac cgggcggcga aggcgtcgat ggcctcctgg
      841 tcctgcatgc ccatgccgcc gttcgggtcc tgcgcccggt tgtcgaaata ccagtactgc
      901 gtctcgccaa actggaggat caccggcagg cccgcggccg cgatctcgtc ggcgcactcc
      961 ttgtacacct gccgcaggta gttgcggacg cgtgagccga agtgcatctg gtgtgacggg
     1021 atttcgaggt acacgtcgac gccaggctgg accacgccgt ccaccagcga gaggtacctg
     1081 gcccgcatgg gcatgggcgg gttgtagcat tccatcgaga aagcaaagga tgcgttaacg
     1141 tcagcctgct ggaactcgcg cgcgagatcg cgaatccaca tccgggcgcc ttctgtcatg
     1201 acgggcgaga cggaatcgat catctcccaa tcgccctcgt tgcccggagt gccgaggttg
     1261 ttcgtaagcg tcatcgtcac ggagttggcc gtgccgacgc tgatggtcgg gaaggtccac
     1321 gtgtgcttga gagcccggcg atgcagcagt acctcagaag aatgcagacg cgtgcggcac
     1381 agcagttgta caaaagacgt tccgaaatcg cggagtttcc gcatatgtgg gccaaagcag
     1441 tgaagaagtg gacgcggttc tcggttcgtg gcctcgccaa ggcgggaatg gaggcgctct
     1501 ggctcgcctt cgcctacaac atcagccagt ggagtaccct ggcgagagcc gcctaaccaa
     1561 gcctccgccg aaggtaagcc gctgtaattg gccacaacgc ctcgccggct gcccctaaac
     1621 tgcgctacgg tcgccaacta cccaaattcc caccaaacca tgcagcccca gcgccctggt
     1681 cgtggccaga gcagcaaccg acgcgcctga agtcctgcgg ctggtatgaa tatttcacgg
     1741 tcccgcaagt gagcggacag cagaaattct acaattgaat aaaaggggcg actgtggcga
     1801 tctatacgtc gtttgagatg gtcgaggact gtaaagcggg cttgcggcgc ggctgggtgt
     1861 attttgtttc ggagttcgaa cctgtaatag ccgcgctggc tgcgcactat tgtgcggaac
     1921 gctatgcctc gaagccttct acggactttc tccgcgacct gcacaccggc gaattctttc
     1981 aatctgtgcg ccccgccaca cagcgtgaat ttgccatcga gttgcggcag gcggtgcttg
     2041 cgctgttgga agcggcctgc cccactcccg cgccggacat cgagttatcg ctcgaagatg
     2101 tcaccacggc gctcgcaccg ctgacccctg tcgaaaaaca gatggcatgg ctcgagacca
     2161 tgaaatacga gccggcccat gccgctctca cgatgaacgc cgggacggat acagtggagc
     2221 gcctgcgtga gaaggttgag gaactgctgc gccagagcct ggaccgctgg aaacgcggtc
     2281 tgcttcgtga aaacggcctg caactgcggg ccgaggcagc cgcccggagc agccaggact
     2341 gcgtacctgt aaagctgttt ctcgatatcc tcgacggacg cgttacatgg tcaaatcgtc
     2401 agaacatcga ccggcacctc gcttcgtgct ggcactgcat tgaccgcttt tgccgcactc
     2461 gcgagatcga ccgccttgtg aaagatacgc cgccgctgac tgccgaagga accgacgccc
     2521 acctcgaaaa gcttggtttt cccaaggaga aagcaccctt ctggaaacgc attctggcgc
     2581 ggtaaagcga ttgcgctaac tttagaggcc gctcccggtc aaaacagcag cttcagcgcg
     2641 agttggacaa aacgcgggtc gagcgtcgag gatacgcggc caaagtttgc gctgctcaag
     2701 tctacagtcg gcgctgtaaa gtttgtcttg ttgaagaggt tgtagaattc gccgcggaat
     2761 tgaagtctcg ctccttcgtt gatgaatgga aggatgaagt tcttagcgaa taccatgttt
     2821 gtctgataga gcggaaaccc ataaaccacg ttgcggccca ggttgccgaa aggagacgtc
     2881 accggcggga tcgccagcgc ggagcggttg aagtaagcga gatgccgctc catggtgctg
     2941 tcgtcatttt tgatcttgcc gccgagataa ttcggacgaa gagccacgtt gccgaggaag
     3001 tccgcctgac cgtcagagag aatgcccgat gaatcgggat agcggatgtt cagcggctga
     3061 ccgctcagca gtgtgacgat accggacact tgccatccac caacgattcc ttcgacaaca
     3121 gcaggcatcg aagtggcgaa ccgccggcct tttccgaaag gcagattgta caccagtgag
     3181 gtggttgagt tgaagcgctg atcgaaactc gaggcgccct tgtcattacg gatgttgtga
     3241 atgtcctgcg ggttcggcga accgccatta gtggtctcga ggacctgtcc aacggtgtcg
     3301 atcgccttcg agtaagtgaa ggagctgatg accgacaggt cgcgtccgcg gtgctcaaac
     3361 ttcatctgca aaccgtggta gcgcgagaag ccgtcaggca cgaccgcggt gatgttattc
     3421 cacccctgat acggccgccg ggcgagcagg gtgacgagcg tcgtcaggcc cgccgagagt
     3481 ttctgctgag tgatgggccg cgcctgattc aggtcgccga gcacgggaag ccgcgagcct
     3541 ctgtttccga cataggcgac gtcgagcagc gtgtctttga acaacgtccg ccggatggtg
     3601 aagtgccagt tctgaacata gccccagggt gtatcgggat ccatgtaaag aatcacattg
     3661 gacggcaggt cgcccgggta tccggcctcg cgtggccgga agcagcctga gtaactgttg
     3721 ccggtacaga tattgccgag gttggctgcc gaattcaccg tagagaagcg cgtcacatat
     3781 ggagcattcg tgttgagata ctcggccgac gccatgcgat tccagaagtt ccaaccgagc
     3841 gcgtagccgc cgcgaatcac cgtgttcgca ttcaaacgat acgcggcccc gagccttggg
     3901 gcgaagttat tgtacggctg ccgctgtaag gagcggtcgt cgccggatgc catcttgagg
     3961 ctgttggtgg acggatcgaa gttggcgagc ctgtcgtcgg cgtcgcgcgc cggagtcatg
     4021 atgtcgtacc gcagcccggc gttgacagta agcttctcac taactttcca gtcgtcctgc
     4081 acataaaaga agttgcatgc tggcgcaccc gggcctgcac ctgcgtggcg agctgatact
     4141 ggctgcgcgc gccgaagaag aagtcggcaa ggtggtacca ggaattgtta acgcctgacg
     4201 gaactccggt tgaagggcgc gagtatgcac cgccgaatga atcgataacg tacaaaggat
     4261 tggtatcgtc cacggtgatg tagaggccga gccactcgta gccggtcttg agattgtggc
     4321 ggccagcggt ccgggagtag ttaagacgcg aattgatcga cgtcgggaac tgcgcttgcg
     4381 ggttcgtcga ttggcggcca tagcggggga atccattgat gtcctgggga gtaatcccac
     4441 cgcgaacctt gtcgccggtg ggcaggccgg tgatcccgaa aagctcttcc atcgacggac
     4501 caccgacggc agccggcgtc cggtccatgc cgaggcgtgt gatgccaagc cggtactcga
     4561 cta
//
